bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring the Influence of Biochar-Supported Nano-Iron Oxide on Phosphorus Speciation Transformation and Bacterial Community Structure in Aerobic Pig Manure Composting Processes
Authors: Yuan Ning , Wang Kang , Liang Mengyue , Zhou Jia , Yu Rui
Published: December 2024

Existing studies have demonstrated the positive effects of nano-sized iron oxide on compost maturity, yet the impact of nano-sized iron oxide on phosphorus speciation and bacterial communities during the composting process remains unclear. In this study, pig manure and straw were used as raw materials, with biochar-supported nano-sized iron oxide (BC-Fe3O4NPs) as an additive and calcium peroxide (CaO2) as a co-agent, to conduct an aerobic composting experiment with pig manure. Four treatments were tested: CK (control), F1 (1% BC-Fe3O4NPs), F2 (5% BC-Fe3O4NPs), and F3 (5% BC-Fe3O4NPs + 5% CaO2). Key findings include the following. (1) BC-Fe3O4NPs increased compost temperatures, with F3 reaching 61℃; F1 showed optimal maturity (C/N ratio: 12.90). (2) BC-Fe3O4NPs promoted stable phosphorus forms; Residual-P proportions were higher in F1, F2, and F3 (25.81%, 51.16%, 51.68%) than CK (19.32%). (3) Bacterial phyla Firmicutes, Actinobacteria, and Proteobacteria dominated. BC-Fe3O4NPs altered community composition, especially on day 7. Firmicutes dominated CK, F1, and F3; Proteobacteria dominated F2. At the genus level, day 7 showed Corynebacterium (CK), Clostridum (F1, F3), and Caldibacillus (F2) as predominant. (4) Pearson correlation analysis revealed shifted correlations between phosphorus forms and bacterial phyla after BC-Fe3O4NPs addition. Firmicutes positively correlated with NaOH-OP in F1 during the thermophilic phase, facilitating phosphate release and adsorption by BC-Fe3O4NPs. The significance of correlations diminished with increasing additive concentration; in F3, all phyla positively correlated with various phosphorus forms.
The Controlling Effect of CaCO3 Supersaturation over Zn Carbonate Assemblages: Co-Crystallization in Silica Hydrogel
Authors: Jorge Pinto André , Sánchez-Pastor Nuria , Fernández-González Angeles
Published: December 2024

Weathering products of sphalerite-bearing ores play an important role in controlling the fate of Zn in the environment. In this framework, the relative stability of Zn carbonates is of special relevance for the common case of ore weathering by carbonated groundwater in the presence of calcium carbonates. We investigated the experimental (co)nucleation and growth of Zn and Ca carbonates at 25 °C in finite double diffusion silica hydrogel media with the purpose of deciphering the system’s reactive pathway and unraveling the major governing factors behind the obtained mineral assemblages. The crystallized solids were carefully extracted two months post-nucleation and studied with micro-Raman spectroscopy, micro X-ray diffraction (XRD), scanning electron microscopy, and electron microprobe (EMP) methods. The obtained results indicate that the grown Zn-bearing phases corresponded to smithsonite and/or Zn hydroxyl carbonate, while CaCO3 polymorphs aragonite and calcite were also crystallized. Moreover, the observed mineral textural relationships reflected the interplay between supersaturation with respect to CaCO3/pCO2 and the grown Zn-bearing carbonate. Experiments conducted in more supersaturated conditions with respect to CaCO3 polymorphs (higher pCO2) favored the precipitation of smithsonite, while the opposite was true for the obtained Zn hydroxyl carbonate phase. The gathered Raman, XRD, and EMP data indicate that the latter phase corresponded to a non-stoichiometric, poorly crystalline solid.
Sedimentological and Geochemical Evaluation of the Lower Cretaceous Yamama Formation, Riyadh, Saudi Arabia: An Integrated Tool for Paleoenvironmental Interpretation
Authors: Khalil Rayan
Published: December 2024

Geochemical proxies are a reliable tool in deciphering the paleoenvironment and diagenetic alteration in carbonate rock units. The Lower Cretaceous Yamama Formation (LCYF) is an important carbonate unit of the Saudi Arabia region which has been studied in detail to evaluate the paleoenvironment and diagenetic alteration through geochemical studies. This study presents new data on petrography, stable isotopes, and trace and rare-earth elements to enhance our understanding on paleoenvironments, redox conditions, and paleosalinity during the deposition of these carbonate units. Field studies show that the formation is composed of thick-to-thin-bedded limestone. Petrographic studies show that the formation is mostly composed of mudstone, wackestone, packstone, and grainstone facies. The stable isotopic values of carbon (δ13C V-PDB = +0.58‰ to +2.23‰) and oxygen (δ18O V-PDB = −6.38‰ to −4.48‰) are directly within the range of marine signatures. CaCO3’s dominance over SiO2 and Al2O3 indicates minimal detrital contribution during the LCYF precipitation. The REE pattern suggests coeval marine signatures which include (i) a slight LREE depletion compared to HREEs (av. Nd/YbN = 0.70), (ii) negative Ce anomalies (av. Ce/Ce* = 0.5), and (iii) a positive La anomaly (av. La/La* = 1.70). Micritic limestone has low Hf (bdl to 0.4 µg/g), Sc (bdl to 2.5 µg/g), and Th (bdl to 0.8 µg/g) content, which suggests negligible detrital influence. The Ce content of different facies (Ce = 1u.80 to 12.85 µg/g) suggests that their deposition took place under oxic to dysoxic conditions. However, there is moderate variation during the deposition of MF-I, with higher Ce values as compared to MF-II, MF-III, and MF-IV, which suggests that the deposition of MF-I mostly took place in anoxic to dysoxic conditions.
The Effect of Pre–Triassic Unconformity on a Hydrocarbon Reservoir: A Case Study from the Eastern Mahu Area, Northwestern Junggar Basin, China
Authors: Tang Yong , Wei Xiaosong , Yan Detian , Zheng Menglin , Zhang Lei , Yu Zhichao
Published: December 2024

Unconformities are of significant interest to petroleum geologists because of their crucial roles in influencing reservoir quality and controlling oil and gas migration. This study investigates the impact of unconformities on a reservoir within a prolific oil–gas-bearing zone between the Middle Permian and Lower Triassic strata in the northwestern Junggar Basin, utilizing thin sections, well logging data, seismic profiles, and geochemical analyses. The results reveal a well-developed three-layer unconformity structure characterized by a thick weathered clay layer, which acts as an effective caprock for hydrocarbons. The diagenetic evolution of the Lower Wuerhe Formation in the northwestern Junggar Basin consists of an initial stage of compaction followed by a subsequent stage of dissolution and cementation. Four key factors, including low argillaceous content in sandstone and conglomerate, diagenetic compaction, zeolite dissolution and cementation, and clay mineral infill, have played a crucial role in influencing the reservoir characteristics of the Lower Wuerhe Formation. In addition, the development of unconformities promotes atmospheric freshwater leaching, which enhances the dissolution of the underlying reservoir while developing an extensive network of strike-slip faults that improve connectivity within hydrocarbon reservoirs. This process facilitates both vertical and lateral migration of hydrocarbons along hard rock layers, which allows the unconformity to breach into the overlying conglomerate reservoirs. The results of this study suggest that the reservoir in proximity to the unconformity surface often exhibits high porosity and rich hydrocarbon content, offering valuable insights for future oil and gas exploration and development.
Heteroleptic Coumarin-Based Silver(I) Complexes: Possible New Antimicrobial Agents
Authors: Mooney Erika , Twamley Brendan , Cooke Gordon , Caraher Emma , Tacke Matthias , Kelleher Fintan , Creaven Bernadette S.
Published: December 2024

Heteroleptic coumarin-based silver(I) complexes with improved solubility profiles were synthesised using either triphenylphosphine or an N-heterocyclic carbene as adduct ligands, and were fully characterised using IR and NMR spectroscopy, elemental analysis, and, where possible, X-ray crystallography. The triphenylphosphine adducts formed well-resolved structures, where the oxyacetate ligands asymmetrically chelated the silver(I) ion in a bidentate chelating mode, and the silver(I) ion was also bound to two triphenylphosphine ligands. The solubility profile and photostability of the adducts were considerably improved compared to those of previously isolated simple coumarin silver(I) complexes. Analysis of the coumarin N-heterocyclic carbene(NHC) silver(I) adduct indicated that it likely formed as a complex aggregate species with an overall stoichiometry of 1:1:1 coumarin:Ag(I):NHC. The Kirby Bauer assay and broth microdilution assays were used to assess the silver(I) complexes’ and adducts’ antimicrobial activity against pathogenic strains of Pseudomonas aeruginosa, Escherichia coli, and MRSA. Interestingly, the formation of more soluble complexes did not increase the activity of the silver(I) complexes and, in effect, made them less effective antimicrobial agents, particularly against Escherichia coli and Pseudomonas aeruginosa, although they retained their activity against MRSA.
Review on Gallium in Coal and Coal Waste Materials: Exploring Strategies for Hydrometallurgical Metal Recovery
Authors: Rudnik Ewa
Published: December 2024

Gallium, a critical and strategic material for advanced technologies, is anomalously enriched in certain coal deposits and coal by-products. Recovering gallium from solid residues generated during coal production and utilization can yield economic benefits and positive environmental gains through more efficient waste processing. This systematic literature review focuses on gallium concentrations in coal and its combustion or gasification by-products, modes of occurrence, gallium-hosting phases, and hydrometallurgical recovery methods, including pretreatment procedures that facilitate metal release from inert aluminosilicate minerals. Coal gangue, and especially fly ashes from coal combustion and gasification, are particularly promising due to their higher gallium content and recovery rates, which can exceed 90% under optimal conditions. However, the low concentrations of gallium and the high levels of impurities in the leachates require innovative and selective separation techniques, primarily involving ion exchange and adsorption. The scientific literature review revealed that coal, bottom ash, and coarse slag have not yet been evaluated for gallium recovery, even though the wastes can contain higher gallium levels than the original material.
[2+2]-Photocycloadditions of 1,4-Naphthoquinone Under Batch and Continuous-Flow Conditions
Authors: Yaseen Madyan A. , Guo Zhifang , Junk Peter C. , Oelgemöller Michael
Published: December 2024

A series of [2+2]-photocycloadditions of 1,4-naphthoquinone with various alkenes and diphenylacetylene were investigated under batch and continuous-flow conditions. Acetone-sensitization furnished the corresponding photoadducts in good to excellent yields and purities. Compared to batch operations that demanded exhaustive irradiation times of 10–13 h, the flow process generally gave superior conversions and subsequently yields with a residence time of just 60 min. The structures of several photoaddition products were further determined by crystal structure analysis.
Porous Carbon Fabricated by Microbial Pretreatment of Brewer’s Grain for the Improvement of Toluene Adsorption Performance
Authors: Wang Jingxin , Wang Xiaohong , Lin Xiaoping , Yu Ziyi , Vione Davide , Huang Haomin , Zhang Xiaohong , Zhang Yanhong , He Jiaqi , Xia Yun , Fang Hansun
Published: December 2024

Porous activated carbons (AC-AN and AC-AO) for toluene adsorption were prepared starting from brewer’s grain biomass pretreated with microorganisms (Aspergillus niger van Tieghem for AC-AN and Aspergillus oryzae RIB40 for AC-AO). The structures and chemical properties of the three activated carbon materials (AC-AN, AC-AO, and AC that was not pretreated with microorganisms) were characterized by N2 adsorption–desorption isotherms, scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), Raman spectroscopy, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The adsorption behavior of the three activated carbons for toluene was studied and correlated with the physical and chemical properties of these materials. The results suggested that the activated carbons prepared by microbial pretreatment had a rougher morphology, higher specific surface area, richer pore structure, fewer oxygen-containing functional groups on the surface, and better adsorption performance for toluene (increased by 31.5% and 18.3% with AC-AN and AC-AO, respectively) compared with the untreated activated carbon (AC). The Thomas model was used to fit the toluene adsorption data, indicating that the rich pore structure accelerated the kinetic process of toluene adsorption. Therefore, appropriate microbial pretreatment of the feedstock that is used to prepare activated carbon can effectively improve its adsorption capacity towards toluene.
Kanamycin and G-Quadruplexes: An Exploration of Binding Interactions
Authors: Gualtieri Gianmarco , Citriniti Emanuele Liborio , Rocca Roberta , Arciuolo Valentina , Amato Jussara , Randazzo Antonio , Alcaro Stefano
Published: December 2024

G-quadruplexes (G4s) are distinctive four-stranded nucleic acid structures formed by guanine-rich sequences, making them attractive targets for drug repurposing efforts. Modulating their stability and function holds promise for treating diseases like cancer. To identify potential drug candidates capable of interacting with these complex DNA formations, docking studies and molecular dynamics (MDs) simulations were conducted. Our analysis revealed kanamycin’s ability to bind to various G4 structures, offering valuable insights into its potential as a modulator of G4 activity. Kanamycin exhibited favorable interactions with both parallel and hybrid G4 topologies in human structures, suggesting a broader mechanism of action for aminoglycosides. These findings may also shed light on aminoglycoside-associated toxicities, indicating that their effects might extend to binding non-ribosomal RNA structures. In summary, this research highlights kanamycin’s potential as a promising tool for influencing G4 dynamics, paving the way for innovative therapeutic strategies targeting G4-related pathways.
Thermal, Molecular Dynamics, and Mechanical Properties of Poly(Ethylene Furanoate)/Poly(ε-Caprolactone) Block Copolymers
Authors: Stanley Johan , Klonos Panagiotis A. , Teknetzi Aikaterini , Rekounas Nikolaos , Kyritsis Apostolos , Fras Zemljič Lidija , Lambropoulou Dimitra A. , Bikiaris Dimitrios N.
Published: December 2024

This study presents the synthesis and characterization of a series of multiblock copolymers, poly(ethylene 2,5-furandicarboxylate)-poly(ε-caprolactone) (PEF-PCL), created through a combination of the two-step melt polycondensation method and ring opening polymerization, as sustainable alternatives to fossil-based plastics. The structural confirmation of these block copolymers was achieved through Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), ensuring the successful integration of PEF and PCL segments. X-ray Photoelectron Spectroscopy (XPS) was employed for chemical bonding and quantitative analysis, providing insights into the distribution and compatibility of the copolymer components. Differential Scanning Calorimetry (DSC) analysis revealed a single glass transition temperature (Tg), indicating the effective plasticizing effect of PCL on PEF, which enhances the flexibility of the copolymers. X-ray Diffraction (XRD) studies highlight the complex relationship between PCL content and crystallization in PEF-PCL block copolymers, emphasizing the need to balance crystallinity and mechanical properties for optimal material performance. Broadband Dielectric Spectroscopy (BDS) confirmed excellent distribution of PEF-PCL without phase separation, which is vital for maintaining consistent material properties. Mechanical properties were evaluated using Nanoindentation testing, demonstrating the potential of these copolymers as flexible packaging materials due to their enhanced mechanical strength and flexibility. The study concludes that PEF-PCL block copolymers are promising candidates for sustainable packaging solutions, combining environmental benefits with desirable material properties.
Enhance the Surface Insulation Properties of EP Materials via Plasma and Fluorine-Containing Coupling Agent Co-Fluorinated Graphene
Authors: Dong Manling , Yang Zhifei , Xia Guowei , Zhang Jiatao , Zhan Zhenyu , Xin Weifeng , Wang Qilin , Xu Bobin , Zhang Yujin , Xie Jun
Published: December 2024

Epoxy resin (EP) is an outstanding polymer material known for its low cost, ease of preparation, excellent electrical insulation properties, mechanical strength, and chemical stability. It is widely used in high- and ultra-high-voltage power transmission and transformation equipment. However, as voltage levels continue to increase, EP materials are gradually failing to meet the performance demands of operational environments. Thus, the development of high-performance epoxy resin materials has become crucial. In this study, a combined treatment using plasma and a fluorine-containing coupling agent was employed to fluorinate graphene nanosheets (GNSs), resulting in DFGNSs. Different concentrations of GNSs/DFGNS-modified EP composites were prepared, and their effects on enhancing the surface insulation properties were studied. Tests on surface flashover voltage, surface charge dissipation, trap distribution, and surface resistivity demonstrated that both GNSs and DFGNSs significantly improve the insulation properties of EP materials. Optimal improvement was achieved with a DFGNS content of 0.2 wt%, where the flashover voltage increased by 16.23%.
A Numerical Simulation Study of the Impact of Kesterites Hole Transport Materials in Quantum Dot-Sensitized Solar Cells Using SCAPS-1D
Authors: Jakalase Sindisiwe , Nqombolo Azile , Meyer Edson L. , Agoro Mojeed A. , Rono Nicholas
Published: December 2024

Energy generation and storage are critical challenges for developing economies due to rising populations and limited access to clean energy resources. Fossil fuels, commonly used for energy production, are costly and contribute to environmental pollution through greenhouse gas emissions. Quantum dot-sensitized solar cells (QDSSCs) offer a promising alternative due to their stability, low cost, and high-power conversion efficiency (PCE) compared to other third-generation solar cells. Kesterite materials, known for their excellent optoelectronic properties and chemical stability, have gained attention for their potential as hole transport layer (HTL) materials in solar cells. In this study, the SCAPS-1D numerical simulator was used to analyze a solar cell with the configuration FTO/TiO2/MoS2/HTL/Ag. The electron transport layer (ETL) used was titanium dioxide (TiO2), while Cu2FeSnS4 (CFTS), Cu2ZnSnS4 (CZTSe), Cu2NiSnS4 (CNTS), and Cu2ZnSnSe4 (CZTSSe) kesterite materials were evaluated as HTLs. MoS2 quantum dot served as the absorber, with FTO as the anode and silver as the back metal contact. The CFTS material outperformed the others, yielding a PCE of 25.86%, a fill factor (FF) of 38.79%, a short-circuit current density (JSC) of 34.52 mA cm−2, and an open-circuit voltage (VOC) of 1.93 V. This study contributes to the advancement of high-performance QDSSCs.
Controllable Growth of Monolayer and Bilayer WSe2 by Liquid-Phase Precursor via Chemical Vapor Deposition for Photodetection
Authors: Wang Siyuan , Wang Pinyi , Tang Hailun , Yu Shilong , Ye Huihui , Fang Xinyu , Ding Jing , Yang Yang , Li Hai
Published: December 2024

Two-dimensional WSe2 nanosheets have received increasing attention due to their excellent optoelectronic properties. Solid precursors, such as WO3 and Se powders, have been extensively employed to grow WSe2 nanosheets by the chemical vapor deposition (CVD) method. However, the high melting point of WO3 results in heterogeneous nucleation sites and nonuniform growth of the WSe2 nanosheet. By dissolving WO3 powder in a NaOH solution, we report a facile and uniform growth of monolayer and bilayer WSe2 nanosheets on a SiO2/Si substrate at a large scale using liquid precursor by the CVD method. The size and thickness of the WSe2 nanosheets were controlled by modulating the precursor concentration and growth temperature. The as-prepared monolayer and bilayer WSe2 nanosheets were well characterized by optical microscopy, atomic force microscopy, and Raman and photoluminescence spectroscopy. With the increase in precursor concentration, the size of the monolayer WSe2 increased up to 120 μm. Bilayer WSe2 nanosheets were grown at higher temperatures. The photosensitivity of the bilayer WSe2 was one order of magnitude higher than that of the monolayer WSe2. The carrier mobility, specific detectivity, photoresponsivity, and external quantum efficiency of the bilayer WSe2 were about two orders of magnitude higher than those of the monolayer WSe2. Our method opens up a new avenue to grow monolayer and bilayer WSe2 for optoelectronic applications.
Recent Developments in Photofunctional Nanomaterials and Nanostructures for Emitting, Manipulating, and Harvesting Light
Authors: Gan Zhixing
Published: December 2024

Photofunctional nanomaterials and nanostructures that can emit, manipulate, convert, and utilize photons in diverse forms have profound meanings, from fundamental understandings to applications [...]
Bimetallic Mesoporous MCM-41 Nanoparticles with Ta/(Ti, V, Co, Nb) with Catalytic and Photocatalytic Properties
Authors: Parvulescu Viorica , Petcu Gabriela , Apostol Nicoleta G. , Atkinson Irina , Petrescu Simona , Baran Adriana , Culita Daniela C. , Ene Ramona , Trica Bogdan , Anghel Elena M.
Published: December 2024

Bimetallic (Ta/Ti, V, Co, Nb) mesoporous MCM-41 nanoparticles were obtained by direct synthesis and hydrothermal treatment. The obtained mesoporous materials were characterized by XRD, XRF, N2 adsorption/desorption, SEM, TEM, XPS, Raman, UV-Vis, and PL spectroscopy. A more significant effect was observed on the mesoporous structure, typically for MCM-41, and on optic properties if the second metal (Ti, Co) did not belong to the same Vb group with Ta as V and Nb. The XPS showed for the TaTi-MCM-41 sample that framework titanium is the major component. The new nanoparticles obtained were used as catalysts for oxidation with hydrogen peroxide of olefinic compounds (1,4 cyclohexadiene, cyclohexene, styrene) and photodegradation of organic pollutants (phenol, methyl orange) from water. The results showed improvementsin activity and selectivity in oxidation reactions by the addition of the second metal to the Ta-MCM-41 catalyst. The slow addition of H2O2 was also beneficial for the selectivity of epoxide products and the stability of the catalysts. The band gap energy values decreased in the presence of the second metal, and the band edge diagram evidenced positive potential for all the conduction bands of the bimetallic samples. The highestlevels of photocatalytic degradation were obtained for the samples with TaTi and TaV.
Tualang Honey Has a Protective Effect Against Photodamage and Skin Cancer: An In Vivo Study
Authors: Sherwani Mohammed Asif , Burns Erin M. , Ahmad Israr , Jasser Ahmed Omar , Chandra Ariq , Yusuf Nabiha
Published: December 2024

Background/Objective: Ultraviolet (UV) B radiation leads to DNA damage by generating cyclobutane pyrimidine dimers (CPDs). UVB-induced CPDs can also result in immune suppression, which is a major risk factor for non-melanoma skin cancer (NMSC). UVB-induced CPDs are repaired by nucleotide repair mechanisms (NER) mediated by xeroderma pigmentosum complementation group A (XPA). The purpose of this study was to investigate the use of TH as a chemopreventive agent against the development of skin cancer. Method: SKH-1 hairless mice were exposed were fed with TH (0.1% v/v) for two weeks and exposed to a single dose of UVB (180 mJ/cm2). Dorsal skin was harvested 24 h post-UVB exposure for evaluation of DNA damage and repair. Lymph nodes were also harvested to prepare single cell suspension for flow cytometric evaluation. For carcinogenesis experiments, SKH-1 hairless mice were given TH (0.1% v/v) ad libitum and exposed to UVB (180 mJ/cm2) thrice a week for 30 weeks. Results: Feeding SKH-1 hairless mice with TH (0.1% v/v) for two weeks prior to a single dose of UVB (180 mJ/cm2) led to a significant increase in XPA in skin and DNA repair cytokines IL-12 and IL-23 in draining lymph nodes. Furthermore, when subjected to the photocarcinogenesis protocol; mice fed with TH developed significantly fewer tumors in comparison to mice fed on drinking water. Conclusions: Our data demonstrate that TH has a protective effect against UVB-induced DNA damage, immune suppression, and skin cancer. Future studies will further investigate the potential of TH as a preventive treatment for NMSC.
Erythrocyte Membrane Fluidity and Omega-3 Fatty Acid Intake: Current Outlook and Perspectives for a Novel, Nutritionally Modifiable Cardiovascular Risk Factor
Authors: Capece Umberto , Gugliandolo Shawn , Morciano Cassandra , Avolio Adriana , Splendore Amelia , Di Giuseppe Gianfranco , Ciccarelli Gea , Soldovieri Laura , Brunetti Michela , Mezza Teresa , Pontecorvi Alfredo , Giaccari Andrea , Cinti Francesca
Published: December 2024

Omega-3 fatty acids reduce triglycerides and have several positive effects on different organs and systems. They are also found in the plasma membrane in variable amounts in relation to genetics and diet. However, it is still unclear whether omega-3 supplementation can reduce the occurrence of major cardiovascular events (MACEs). Two trials, REDUCE-IT (Reduction of Cardiovascular Events with Icosapent Ethyl-Intervention Trial), with highly purified EPA, and STRENGTH (Effect of High-Dose Omega-3 Fatty Acids vs. Corn Oil on Major Adverse Cardiovascular Events in Patients at High Cardiovascular Risk), with a combination of EPA and DHA, have produced different outcomes, triggering a scientific debate on possible explanations for the discrepancies. Furthermore, doubts have arisen as to the anti-inflammatory and anti-aggregating activity of these compounds. Recent studies have, however, highlighted interesting effects of EPA and DHA on erythrocyte membrane fluidity (EMF). EMF is governed by a complex and dynamic biochemical framework, with fatty acids playing a central role. Furthermore, it can be easily measured in erythrocytes from a blood sample using fluorescent probes. Recent research has also shown that EMF could act as a possible cardiovascular risk factor biomarker. This review aims to synthetize the latest evidence on erythrocyte membrane fluidity, exploring its potential role as a biomarker of residual cardiovascular risk and discussing its clinical relevance. Further, we aim to dissect the possible biological mechanisms that link omega-3 modifiable membrane fluidity to cardiovascular health.
Anti-Inflammatory and Anticancer Effects of Kaurenoic Acid in Overcoming Radioresistance in Breast Cancer Radiotherapy
Authors: Kim Tae Woo , Ko Seong-Gyu
Published: December 2024

Background/Objectives: Peroxisome proliferator–activated receptor γ (PPARγ) plays a key role in mediating anti-inflammatory and anticancer effects in the tumor microenvironment. Kaurenoic acid (KA), a diterpene compound isolated from Sphagneticola trilobata (L.) Pruski, has been demonstrated to exert anti-inflammatory, anticancer, and antihuman immunodeficiency virus effects. Methods: In this study, we identified KA as a novel activator of PPARγ with potent anti-inflammatory and antitumor effects both in vitro and in vivo. Given the potential of PPARγ regulators in overcoming radioresistance and chemoresistance in cancer therapies, we hypothesized that KA may enhance the efficacy of breast cancer radiotherapy. Results: In a lipopolysaccharide (LPS)-induced mouse inflammation model, KA treatment reduced the levels of pro-inflammatory cytokines, including COX-2, IL-6, IL-1β, and TNFα. In a xenograft mouse mode of breast cancer, KA treatment inhibited tumor growth. Specifically, KA treatment enhanced caspase-3 activity and cytotoxicity against MDA-MB-231 and MCF-7 breast cancer cells. When KA was co-treated with a caspase inhibitor, Z-VAD-FMK, caspase-dependent apoptosis was suppressed in these cells. KA was found to induce the generation of cytosolic calcium ions (Ca2+) and reactive oxygen species (ROS), triggering endoplasmic reticulum (ER) stress via the PERK-ATF4-CHOP axis. Hence, the ER stressor thapsigargin (TG) synergized with KA treatment to enhance apoptosis in these cells, while the loss of the PERK or CHOP function inhibited this phenomenon. KA treatment was shown to induce oxidative stress via the NADPH oxidase 4 (NOX4) and stimulate ROS production. Specifically, NOX4 knockdown (KD) and antioxidant treatment (N-acetyl cysteine or diphenyleneiodonium) suppressed such ER stress–mediated apoptosis by inhibiting KA-enhanced caspase-3 activity, cytotoxicity, and intracellular ROS production in the treated cells. In radioresistant MDA-MB-231R and MCF-7R cells, KA combined with 2 Gy radiation overcame radioresistance by upregulating PPARγ and modulating epithelial–mesenchymal transition (EMT) markers, such as E-cadherin, N-cadherin, and vimentin. In PPARγ KD MDA-MB-231R and MCF-7R cells, this phenomenon was inhibited due to reduced PPARγ and NOX4 expression. Conclusions: In conclusion, these findings demonstrated KA as a novel PPARγ regulator with promising potential to enhance the efficacy of breast cancer radiotherapy.
Potential Effects of Bioactive Compounds of Plant-Based Foods and Medicinal Plants in Chronic Kidney Disease and Dialysis: A Systematic Review
Authors: Josa Esmeralda , Barril Guillermina , Ruperto Mar
Published: December 2024

Background. The bioactive components of plant foods and medicinal plants have attracted interest due to their potential impact on the progression of chronic kidney disease (CKD) and outcomes. Objective. This study aimed to conduct a critical and quantitative systematic review of randomized clinical trials (RCTs) investigating the potential effects of selected phytochemicals from plant-based foods and medicinal plants in CKD and dialysis patients. Methods. The review included studies that related plant-based bioactive compounds (curcumin, propolis, sulforaphane, betalain, catechins, rhein, emodin, aloe-emodin, flavonoids, and triptolide) and medicinal plants (green tea, rhubarb, Astragalus membranaceus, and Tripterygium wilfordii Hook F) in CKD and dialysis patients. A literature search was conducted in PubMed, LILACS, Embase, Scopus, and WOS between December 2022 and October 2024. This review was performed according to the PRISMA flowchart and was registered in PROSPERO (595162). Results. In the eight RCTs conducted with curcumin, anti-inflammatory, antioxidant, and microbiota-modulating properties were reported. As for propolis, in three RCTs, anti-inflammatory, anti-proteinuric, and renal-protective properties were reported. Sulforaphane in one RCT showed antioxidant and cardiovascular benefits, and in another RCT no effects were observed. In one RCT, genistein was shown to be a potential anti-inflammatory agent and improved nutritional status. Allicin in two RCTs showed cardioprotective, antioxidant, anti-inflammatory, and lipid-lowering effects. Finally, beetroot showed a vasodilator effect in one RCT. As for the medicinal plants, green tea, rhubarb, Astragalus membranaceus, and Tripterygium Wilfordii Hook F, in six RCTs they showed antioxidant, anti-inflammatory, cardioprotective, antiproteinuric, and renoprotective properties. Conclusions. These results suggest that bioactive compounds of plant-based foods and medicinal plants have promising effects in terms of preventing or treating CKD progression and appear to improve inflammation and antioxidant capacity and support cardiovascular benefits and renoprotective effects; however, it is recommended that further studies be carried out.
The Impact and Efficacy of Vitamin D Fortification
Authors: Alnafisah Ruyuf Y. , Alragea Atheer S. , Alzamil Mona K. , Alqahtani Amani S.
Published: December 2024

Vitamin D deficiency is a global health issue linked to various chronic diseases and overall mortality. It primarily arises from insufficient sunlight exposure, compounded by dietary limitations. Vitamin D fortification of commonly consumed foods has emerged as a viable public health intervention to address this deficiency. This review evaluates the impact of vitamin D food fortification on serum levels, intake, and health outcomes and explores the stability, bio-accessibility, bioavailability, and cost-effectiveness of such interventions. A comprehensive literature search was conducted in PubMed and Google Scholar, focusing on studies from 2015 to 2024. The criteria included primary research on healthy adults that addressed the effects of vitamin D fortification on health, intake, and serum levels, as well as the fortification’s stability, bio-accessibility, bioavailability, and cost-effectiveness. Studies were extracted and analyzed according to PRISMA guidelines. The review included 31 studies from diverse geographic locations, revealing that fortifying dairy products, cereals, fats, oils, and other food items effectively increased serum 25-hydroxyvitamin D levels. The fortification methods varied, with vitamin D3 showing superior efficacy over vitamin D2. Encapsulation techniques improved stability and bioavailability. Fortifying staple foods like milk and eggs proved cost-effective compared with pharmaceutical interventions. Vitamin D food fortification significantly enhances serum levels and intake, with dairy and cereals being the most frequently fortified. Standardized fortification guidelines are essential to ensure safety and efficacy. Ongoing evaluation and region-specific policies are crucial for effectively optimizing fortification strategies and addressing vitamin D deficiency.
The Impact of Increased Physical Activity at School on the Nutritional Behavior and BMI of 13-Year-Olds
Authors: Ługowska Katarzyna , Krzęcio-Nieczyporuk Elżbieta , Trafiałek Joanna , Kolanowski Wojciech
Published: December 2024

Background/Objectives: Diet and physical activity (PA) significantly impact health. Unfortunately, a worrying trend of decreasing PA among children and adolescents, accompanied by unhealthy nutritional behavior, is observed worldwide. The aim of the study was to evaluate the nutritional behavior and body mass index (BMI) of adolescents aged 13 years in groups of extended and standard PA at school. Methods: The study was conducted in six schools among adolescents born in 2007 (n = 213), in two groups of standard and extended PA at school (SPA and EPA), wherein each group received 4 and 10 h of physical education lessons per week, respectively. Their height and body mass were measured. BMI was calculated and compared with percentile charts. A questionnaire was used to assess nutritional behavior and extracurricular physical activity. Results: Approximately 62% of adolescents had a normal body weight, with SPA 58%, EPA 64%, overweight 13.63%, SPA 13.75%, EPA 13.50%, obesity 14.13%, SPA 19.25%, EPA 9%, and underweight 11.17%, SPA 8.85%, and EPA 13.50%. Among the potential health-promoting foods, dishes, and beverages, fruits and vegetables were the most frequently consumed items, whereas fish and legume dishes were the least consumed. Butter, white bread, sweets, cheeses, cold cuts, and sausages were all consumed very often. Fast food and lard were the least frequently consumed items. Conclusions: A significant proportion of 13-year-olds were overweight or obese. Extended PA had a beneficial effect on BMI, but it had little effect on nutritional behavior among the studied adolescents. The adolescents exhibited moderate nutritional behaviors; however, it was more favorable in those exposed to extended PA at school than those exposed to standard PA. It is possible to argue that extending organized PA at school leads to increased PA during leisure time. It is recommended to increase PA for adolescents by doubling the mandatory number of physical education lessons or other sports activities in the school curriculum.
Associations Between Added Sugars Intake from Various Food and Beverage Sources and Diet Quality Among the U.S. Population
Authors: Ricciuto Laurie , DiFrancesco Loretta , Gaine P. Courtney , Scott Maria O. , Fulgoni Victor L.
Published: December 2024

Background: A diet high in added sugars has been linked to poor diet quality; however, little is known about specific sources of added sugars and their association with diet quality. Objective: This study examined associations between added sugars intake from specific food and beverage sources and diet quality, as indicated by the Healthy Eating Index (HEI) 2020 score, among the U.S. population. Methods: Data from eight consecutive 2-year cycles of the National Health and Nutrition Examination Survey (2003–2004 through 2017–2018) were pooled, and regression analysis was conducted to examine associations between total HEI-2020 score or HEI-2020 component scores and added sugars intake (% kcal) from key contributors: soft drinks, fruit drinks and coffee and tea; ready-to-eat cereals; flavored milk; sweet bakery products; and snack/meal bars. Results: A higher added sugars intake from soft drinks, fruit drinks and coffee and tea was associated with lower diet quality (lower total HEI score and lower scores on most of the HEI components) among both children and adults (p < 0.0001). In contrast, higher added sugars intakes from flavored milk (p < 0.0001) and snack/meals bars (p < 0.001) among children, and from sweet bakery products (p < 0.0001) among adults, were associated with higher diet quality. For all these associations, changes in the total HEI score across quintiles of added sugars intake were very small, ranging from 50.2 to 52.8 for children and 55.4 to 57.5 for adults, depending on the added sugars source. Conclusions: The nature of the relationship between added sugars intake and diet quality depends on the source of added sugars. While the small differences in diet quality may be of limited practical significance, our results suggest that the consideration of the different roles of various added sugars sources in the diet is warranted when developing dietary guidance.
The Association of Dietary Polyamines with Mortality and the Risk of Cardiovascular Disease: A Prospective Study in UK Biobank
Authors: Han Su , Qian Mingxia , Zhang Na , Zhang Rui , Liu Min , Wang Jiangbo , Li Furong , Zheng Liqiang , Sun Zhaoqing
Published: December 2024

Background: Polyamines, including spermidine (SPD), spermine (SPM) and putrescine (PUT), are essential for cellular physiology and various cellular processes. This study aimed to examine the associations of dietary polyamines intake and all-cause mortality and incident cardiovascular disease (CVD). Methods: This prospective cohort study included 184,732 participants without CVD at baseline from the UK Biobank who had completed at least one dietary questionnaire. Diet was assessed using Oxford WebQ, a web-based 24 h recall questionnaire, with polyamines intakes estimated from previous studies. Cox proportional models with restricted cubic splines were employed to investigate nonlinear associations. The primary endpoint was all-cause mortality or incident CVD (including CVD death, coronary heart disease and stroke). Results: During a median follow-up period of 11.5 years, 7348 (3.9%) participants died and 12,316 (6.5%) developed incident CVD. Polyamines intake showed nonlinear associations with all-cause mortality and incident CVD (P for nonlinear < 0.01). Compared to the lowest quintile group of dietary polyamines intake (≤17.4 mg/day), the quintile 2 to 5 groups demonstrated a reduced risk of all-cause mortality, with the lowest risk in quintile 2 group (>17.4–22.3 mg/day) (HR:0.82, 95% CI: 0.76–0.88). Similar results were observed for incident CVD, with the lowest risk in the quintile 4 group (>27.1–33.5 mg/day) (HR: 0.86, 95% CI: 0.82–0.92). Conclusions: We found that dietary polyamines intake was associated with a lower risk of all-cause mortality or incident CVD. Furthermore, our study identified an optimal range of dietary polyamines intake.
Nutraceutical Properties of Thai Mulberry (Morus alba L.) and Their Effects on Metabolic and Cardiovascular Risk Factors in Individuals with Obesity: A Randomized, Single-Blind Crossover Trial
Authors: Parklak Wason , Chottidao Monchai , Munkong Narongsuk , Komindr Surat , Monkhai Sudjai , Wanikorn Bandhita , Makaje Niromlee , Kulprachakarn Kanokwan , Chuljerm Hataichanok , Somnuk Surasawadee
Published: December 2024

Background/Objectives: Mulberries exhibit antioxidant properties that may attenuate metabolic abnormalities. Kamphaeng Saen mulberry (KPS-MB-42-1) contains anthocyanins, polyphenols, and nutrients, but few studies have explored its benefits for human health. This study investigated the effects of a concentrated mulberry drink (CMD) from the KPS-MB-42-1 cultivar on metabolic and cardiovascular risk factors in obese individuals. Methods: A single-blind, randomized crossover clinical pilot trial was performed on individuals with obesity. Participants consumed 100 g of CMD daily, alternating with placebo for 6 weeks. Body composition, blood pressure, and blood samples were assessed at baseline and post-intervention. Results: This study was completed with 12 participants (7 men, 5 women, aged 30–55 years, BMI 32.1 ± 5.98 kg/m2) consuming CMD with 1041.90 mg total phenolic compounds and 35.34 mg total anthocyanins. No significant changes in body composition were observed. CMD consumption significantly reduced systolic and diastolic blood pressure, and mean arterial pressure, compared to baseline and placebo periods (p < 0.05). While total cholesterol, LDL-C, and HDL-C remained unchanged, triglycerides were significantly lower during CMD consumption compared to placebo periods (p < 0.05). Fasting plasma glucose (FPG) levels were stable during CMD consumption but increased significantly with the placebo period (p < 0.05). C-reactive protein levels were also significantly lower during CMD consumption compared to placebo periods (p < 0.05). No changes in blood coagulation indicators (prothrombin time, activated partial thromboplastin time, and the international normalized ratio) were found. Conclusions: CMD improved metabolic markers, particularly regarding its antihypertensive effects. These findings highlight CMD’s potential as a health drink for managing metabolic syndrome and preventing chronic diseases.
Curcumin and Its Potential to Target the Glycolytic Behavior of Lactate-Acclimated Prostate Carcinoma Cells with Docetaxel
Authors: Choi Dongsic , Lee Jun Gi , Heo Su-Hak , Cho Moon-Kyen , Nam Hae-Seon , Lee Sang-Han , Lee Yoon-Jin
Published: December 2024

Background: Dysregulated cellular metabolism is known to be associated with drug resistance in cancer treatment. Methods: In this study, we investigated the impact of cellular adaptation to lactic acidosis on intracellular energy metabolism and sensitivity to docetaxel in prostate carcinoma (PC) cells. The effects of curcumin and the role of hexokinase 2 (HK2) in this process were also examined. Results: PC-3AcT and DU145AcT cells that preadapted to lactic acid displayed increased growth behavior, increased dependence on glycolysis, and reduced sensitivity to docetaxel compared to parental PC-3 and DU145 cells. Molecular analyses revealed activation of the c-Raf/MEK/ERK pathway, upregulation of cyclin D1, cyclin B1, and p-cdc2Thr161, and increased levels and activities of key regulatory enzymes in glycolysis, including HK2, in lactate-acclimated cells. HK2 knockdown resulted in decreased cell growth and glycolytic activity, decreased levels of complexes I–V in the mitochondrial electron transport chain, loss of mitochondrial membrane potential, and depletion of intracellular ATP, ultimately leading to cell death. In a xenograft animal model, curcumin combined with docetaxel reduced tumor size and weight, induced downregulation of glycolytic enzymes, and stimulated the upregulation of apoptotic and necroptotic proteins. This was consistent with the in vitro results from 2D monolayer and 3D spheroid cultures, suggesting that the efficacy of curcumin is not affected by docetaxel. Conclusions: Overall, our findings suggest that metabolic plasticity through enhanced glycolysis observed in lactate-acclimated PC cells may be one of the underlying causes of docetaxel resistance, and targeting glycolysis by curcumin may provide potential for drug development that could improve treatment outcomes in PC patients.
Assessing Lifestyle in a Large Cohort of Undergraduate Students: Significance of Stress, Exercise and Nutrition
Authors: Lucini Daniela , Luconi Ester , Giovanelli Luca , Marano Giuseppe , Bernardelli Giuseppina , Guidetti Riccardo , Morello Eugenio , Cribellati Stefano , Brambilla Marina Marzia , Biganzoli Elia Mario
Published: December 2024

Background/Objectives: Lifestyle (in particular, nutrition and exercise) determines present and future youths’ health. The goal of the present study was to identify specific student groups who deserve precise lifestyle improvement interventions, tailored to their characteristics. Methods: An anonymous web-based questionnaire to assess lifestyle was posted on the websites of two main Italian Academic Institutions, and 9423 students voluntarily participated. A personalised immediate report was provided to improve compliance/motivation. We assessed age, sex, affiliation, anthropometrics, lifestyle components (nutrition, exercise, sedentariness, stress perception, smoking, alcohol, sleep), and the desire to be helped with lifestyle improvement. Cluster analysis was performed to identify healthy lifestyle groups among the students. Results: In total, 6976 subjects [age: 21 (20, 23) yrs; 3665 female, 3300 male] completed the questionnaire and were included. Of these students, 73.9% expressed the need for lifestyle improvement help, particularly for becoming physically active (66.7%), managing stress (58.7%), and improving nutrition (52.7%). We unveil three clusters of subjects, each corresponding to a distinct lifestyle pattern. The clusters are differentiated by exercise level and perceptions of stress/fatigue/somatic symptoms (cluster 1: 74.8% meet international exercise guidelines (IEGs), 67.4% have high stress perception, 49.1% drink 1–3 glasses of wine/beer per week, and 63.3% drink 0–1 glass of spirits per week; cluster 2: 75.6% meet IEGs, 75.7% have low/medium levels of stress perception, and 65.8% have low alcohol consumption; cluster 3: 72.5% do not meet IEGs, 77.6% have high stress perception, and 67.5% have low alcohol consumption). More active students present lower stress/somatic symptoms perception. Interestingly, the AHA diet score (nutrition quality) was not in the ideal range in any cluster (nevertheless, obesity was not of concern), being worst in cluster 3, characterized by higher stress perception (59.7% had poor nutrition quality). Those who were physically active but showed a high stress/fatigue perception were used to drinking alcohol. Conclusions: Students desire help to improve their lifestyle, and this approach might help identify specific student groups to whom LIs in Academic Institutions can be tailored to foster well-being and promote health.