bluuebunny/crossref_metadata_2025 huggingface dataset:
Hyaluronan Sperm Selection during ICSI (HA-ICSI) Increases Embryo Utilization in Couples with Male Factor Infertility
Authors: Williams Ashleigh , Popkiss Sophie , Horta Fabrizzio , McLachlan Robert , Zander-Fox Deirdre , Green Mark P.
Published: February 2024

Background: Hyaluronan (HA) sperm selection, utilizing the affinity of mature sperm for HA, has emerged as a technique to enhance the selection of sperm for intracytoplasmic sperm injection (ICSI). However, conflicting evidence exists regarding the impact of HA-ICSI on clinical outcomes including fertilization, embryo utilization, and pregnancy rates. The study aimed to compare clinical outcomes including fertilization, utilization, and pregnancy rates between paired ICSI and HA-ICSI cycles within patients, particularly for male factor and low utilization subgroups.

Methods: A retrospective study from 3,988 ICSI treatment cycles was conducted between January 2018 and March 2022. A HA-ICSI cycle was paired with the preceding ICSI cycle from the same patient. Fertilization, utilization, and clinical pregnancy rates were compared based on infertility diagnosis and utilization rate (UR) from the initial ICSI cycle.

Results: There were no differences in fertilization, utilization, embryo quality, euploidy, or pregnancy rates between ICSI and HA-ICSI in all paired cycles. However, for patients with male factor infertility UR increased from 25.0% to 38.5% in their HA-ICSI cycle ([Formula: see text]). There was no difference observed for female factor infertility patients. HA-ICSI use was of particular benefit for male factor infertility patients with low ([Formula: see text]) embryo utilization in their initial ICSI cycle, evident by an increased utilization from 14.3% to 25.0% in their HA cycle ([Formula: see text]).

Conclusions: This is the first study to focus on the impact of HA-ICSI on embryo utilization, particularly in patients with male factor infertility. The efficacy of HA-ICSI as a sperm selection tool appears to be limited to improving UR in patients with male factor infertility, particularly those with low utilization in a previous ICSI cycle, with no significant effect on fertilization or pregnancy rates.

Conclusion: HA-ICSI may be useful for sperm selection at the time of ICSI for a select subgroup of patients. Male factor infertility patients, who have previously undertaken an ICSI cycle with less than 40% utilization, should be considered as candidates for treatment.
A model of redistribution of dopant in a multilayer structure with changing overgrowth regime
Authors: Pankratov E. L.
Published: February 2024

In this paper, we analyzed the redistribution of dopants in a multilayer structure during the overgrowing of a doped area. The effect of changing of regimes of overgrowing of the doped area on the redistribution of dopant was analyzed. An analytical approach for analysis of the redistribution of dopant was introduced. The approach makes it possible to take into account the changing parameters of the considered process in space and time together, as well as the nonlinearity of the mass transport.
Understanding the Contribution of Conventional Media in Earthquake Risk Communication
Authors: Tekeli-Yesil Sidika , Tanner Marcel
Published: February 2024

To mitigate the damage from and prepare for disasters, individuals and communities should be involved in the disaster risk reduction efforts. Communicating the risk effectively is the essential step of this involvement. Risk communication is a multidisciplinary field. Disaster managers have to work closely with the media, among other partners, but there is a gap in their understanding of each other’s roles. This study aims to explore the process of publishing and broadcasting earthquake information through the experiences of media professionals, to understand how journalists and news organizations function before and after disasters, and to provide evidence to help bridge the gap between disaster managers and/or public health authorities and disaster journalists and/or news organizations., especially in earthquake preparedness. Thirteen in-depth interviews were conducted with editors/journalists from national newspapers and TV channels. A good context for investigating these issues is afforded by the megacity Istanbul and the Turkish media. Turkey is a land of earthquakes. A further strong earthquake is expected to hit in the near future. The findings, which gave valuable hints for better collaboration, are presented according to the focus of the question guide: time of and norms for publishing or broadcasting news or information about earthquakes; content of the news or information; disaster journalism and guidelines.
Increasing maternal glucose concentrations is insufficient to restore placental glucose transfer in chorionic somatomammotropin RNA interference pregnancies
Authors: Tanner Amelia R. , Kennedy Victoria C. , Lynch Cameron S. , Winger Quinton A. , Anthony Russell V. , Rozance Paul J.
Published: February 2024

The current study demonstrated a novel, critical autocrine role for chorionic somatomammotropin in augmenting placental glucose transfer and maintaining placental oxidative metabolism. In pregnancies with CSH deficiency, excess glucose in maternal circulation is insufficient to overcome fetal hypoglycemia due to impaired placental glucose transfer and elevated placental metabolic demands. This suggests that perturbations in glucose transfer in CSH RNAi pregnancies are due to compromised metabolic efficiency along with reduced placental mass.
Seroprevalence of IgM/IgG and Neutralizing Antibodies against SARS-CoV-2 in Unvaccinated Young Adults from Mexico Who Reported Not Having Had a Previous COVID-19 Infection
Authors: Padilla-Bórquez Diana Lourdes , Matuz-Flores Mónica Guadalupe , Hernández-Bello Jorge , Sánchez-Zuno Gabriela Athziri , García-Arellano Samuel , Oregon-Romero Edith , Herrera-Godina Melva Guadalupe , González-Estevez Guillermo , Adan-Bante Norma Patricia , Rosas-Rodríguez Jesús Alfredo , Muñoz-Valle José Francisco
Published: February 2024

Background. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus that causes coronavirus disease 2019 (COVID-19). It is estimated that more than half of new infections are transmitted by asymptomatic people; therefore, the isolation of symptomatic people is not enough to control the spread of the disease. Methods. A total of 171 unvaccinated young adults (18–35 years) from Sonora, Mexico, who underwent a structured survey to identify prior COVID-19 infections, were included in this study. A qualitative determination of anti-SARS-CoV-2 antibodies in serum was performed by lateral flow immunoassay (Certum IgG/IgM Rapid Test™ cassette kit) and neutralizing antibodies were also determined (GenScript cPass assay). Results. A total of 36 people reported a history of COVID-19 infection, and 135 reported no history of COVID-19. In contrast, 49.6% (67/135) of individuals who had not reported a previous SARS-CoV-2 infection were seropositive to the rapid anti-SARS-CoV-2 antibody test, and 48.1% (65/135) of them had neutralizing antibodies. Conclusions. These results suggest that in young adults, SARS-CoV-2 infections could be asymptomatic in a high percentage of individuals, which could contribute in part to the slow control of the current pandemic due to the large number of asymptomatic cases that are contagious and that could be a silent spread of the virus.
Quercetin Promotes Apoptosis of Gastric Cancer Cells through the EGFR-ERK Signaling Pathway
Authors: Liu Yali , Li Yan , Jiang Yanjun , Zheng Xin , Wang Tianming , Li Jing , Zhang Biyun , Zhu Jiarui , Wei Xintong , Huang Ruihua , Zhang Yong , Jin Qiaoying
Published: February 2024

Previous studies have shown that various active components of licorice have anticancer effects. However, few studies have investigated the mechanism of action of licorice in gastric cancer. The effect of active compounds in licorice on the biological activity of gastric cancer cells was investigated in vitro (MKN-45 cells). Network pharmacology and molecular docking were used to predict the potential targets of licorice against gastric cancer and verify the binding stability of target proteins to compounds. In addition, the anticancer effect of licorice was assessed using a mouse model of gastric cancer. The licorice-active component (quercetin) effectively inhibited proliferation, caused cell cycle arrest, and promoted apoptosis in MKN-45 cells, accompanied by increased Cyt-C, decreased BCL-2, and decreased mitochondrial membrane potential and mitochondrial damage. Further research showed that quercetin targeted EGFR, blocked the ERK signaling pathway, and downregulated PTGS2. In the in vivo experiment, quercetin treatment resulted in reduced tumor volume, decreased Ki67 and BCL-2 expression in tumor tissue, increased caspase 3 and BAX levels, and induced mitochondrial damage.
Supplementary figure S1 from Natural History of Germline BRCA1 Mutated and BRCA Wild-type Triple-negative Breast Cancer
Authors: Gardi Nilesh , Chaubal Rohan , Parab Pallavi , Pachakar Sunil , Kulkarni Suyash , Shet Tanuja , Joshi Shalaka , Kembhavi Yogesh , Chandrani Pratik , Quist Jelmar , Kowtal Pradnya , Grigoriadis Anita , Sarin Rajiv , Govindarajan Raman , Gupta Sudeep
Published: February 2024

This figure shows peaks from Sanger sequencing of germline variants identified in two patient samples.
Supplementary Table S1 from Natural History of Germline BRCA1 Mutated and BRCA Wild-type Triple-negative Breast Cancer
Authors: Gardi Nilesh , Chaubal Rohan , Parab Pallavi , Pachakar Sunil , Kulkarni Suyash , Shet Tanuja , Joshi Shalaka , Kembhavi Yogesh , Chandrani Pratik , Quist Jelmar , Kowtal Pradnya , Grigoriadis Anita , Sarin Rajiv , Govindarajan Raman , Gupta Sudeep
Published: February 2024

This table shows the relevant clinical and assay information for patients on this study.
Shared and Distinct Renal Transcriptome Signatures in 3 Standard Mouse Models of Chronic Kidney Disease
Authors: Marstrand-Jørgensen Adam B. , Sembach Frederikke Emilie , Bak Stine Thorhauge , Ougaard Maria , Christensen-Dalsgaard Mikkel , Rønn Madsen Martin , Jensen Ditte Marie , Secher Thomas , Heimbürger Sebastian Møller Nguyen , Fink Lisbeth N. , Hansen Ditte , Hansen Henrik H. , Østergaard Mette Viberg , Christensen Michael , Dalbøge Louise S.
Published: February 2024

Introduction: Several mouse models with diverse disease etiologies are used in preclinical research for chronic kidney disease (CKD). Here, we performed a head-to-head comparison of renal transcriptome signatures in standard mouse models of CKD to assess shared and distinct molecular changes in three mouse models commonly employed in preclinical CKD research and drug discovery. Methods: All experiments were conducted on male C57BL/6J mice. Mice underwent sham, unilateral ureter obstruction (UUO), or unilateral ischemic-reperfusion injury (uIRI) surgery and were terminated two- and 6-weeks post-surgery, respectively. The adenine-supplemented diet-induced (ADI) model of CKD was established by feeding with adenine diet for 6 weeks and compared to control diet feeding. For all models, endpoints included plasma biochemistry, kidney histology, and RNA sequencing. Results: All models displayed increased macrophage infiltration (F4/80 IHC) and fibrosis (collagen 1a1 IHC). Compared to corresponding controls, all models were characterized by an extensive number of renal differentially expressed genes (≥11,000), with a notable overlap in transcriptomic signatures across models. Gene expression markers of fibrosis, inflammation, and kidney injury supported histological findings. Interestingly, model-specific transcriptome signatures included several genes representing current drug targets for CKD, emphasizing advantages and limitations of the three CKD models in preclinical target and drug discovery. Conclusion: The UUO, uIRI, and ADI mouse models of CKD have significant commonalities in their renal global transcriptome profile. Model-specific renal transcriptional signatures should be considered when selecting the specific model in preclinical target and drug discovery.
Role of
TBX20
Truncating Variants in Dilated Cardiomyopathy and Left Ventricular Noncompaction
Authors: Amor-Salamanca Almudena , Santana Rodríguez Alfredo , Rasoul Hazhee , Rodríguez-Palomares José F. , Moldovan Oana , Hey Thomas Morris , Delgado María Gallego , Cuenca David López , de Castro Campos Daniel , Basurte-Elorz María Teresa , Macías-Ruiz Rosa , Fuentes Cañamero María Eugenia , Galvin Joseph , Bilbao Quesada Raquel , de la Higuera Romero Luis , Trujillo-Quintero Juan Pablo , García-Cruz Loida María , Cárdenas-Reyes Ivonne , Jiménez-Jáimez Juan , García-Hernández Soledad , Valverde-Gómez María , Gómez-Díaz Iria , Limeres Freire Javier , García-Pinilla José M. , Gimeno-Blanes Juan R. , Savattis Konstantinos , García-Pavía Pablo , Ochoa Juan Pablo
Published: February 2024

BACKGROUND:

Less than 40% of patients with dilated cardiomyopathy (DCM) have a pathogenic/likely pathogenic genetic variant identified.
TBX20
has been linked to congenital heart defects; although an association with left ventricular noncompaction (LVNC) and DCM has been proposed, it is still considered a gene with limited evidence for these phenotypes. This study sought to investigate the association between the
TBX20
truncating variant (
TBX20tv
) and DCM/LVNC.

METHODS:

TBX20
was sequenced by next-generation sequencing in 7463 unrelated probands with a diagnosis of DCM or LVNC, 22 773 probands of an internal comparison group (hypertrophic cardiomyopathy, channelopathies, or aortic diseases), and 124 098 external controls (individuals from the gnomAD database). Enrichment of
TBX20tv
in DCM/LVNC was calculated, cosegregation was determined in selected families, and clinical characteristics and outcomes were analyzed in carriers.

RESULTS:

TBX20tv
was enriched in DCM/LVNC (24/7463; 0.32%) compared with internal (1/22 773; 0.004%) and external comparison groups (4/124 098; 0.003%), with odds ratios of 73.23 (95% CI, 9.90–541.45;
P
<0.0001) and 99.76 (95% CI, 34.60–287.62;
P
<0.0001), respectively.
TBX20tv
was cosegregated with DCM/LVNC phenotype in 21 families for a combined logarythm of the odds score of 4.53 (strong linkage). Among 57 individuals with
TBX20tv
(49.1% men; mean age, 35.9±20.8 years), 41 (71.9%) exhibited DCM/LVNC, of whom 14 (34.1%) had also congenital heart defects. After a median follow-up of 6.9 (95% CI, 25–75:3.6–14.5) years, 9.7% of patients with DCM/LVNC had end-stage heart failure events and 4.8% experienced malignant ventricular arrhythmias.

CONCLUSIONS:

TBX20tv
is associated with DCM/LVNC; congenital heart defect is also present in around one-third of cases.
TBX20tv
-associated DCM/LVNC is characterized by a nonaggressive phenotype, with a low incidence of major cardiovascular events.
TBX20
should be considered a definitive gene for DCM and LVNC and routinely included in genetic testing panels for these phenotypes.
The ABC‐Stroke Risk Score and Effects of Atrial Fibrillation Screening on Stroke Prevention: Results From the Randomized LOOP Study
Authors: Xing Lucas Yixi , Diederichsen Søren Zöga , Højberg Søren , Krieger Derk W. , Graff Claus , Frikke‐Schmidt Ruth , Platonov Pyotr G. , Olesen Morten S. , Brandes Axel , Køber Lars , Haugan Ketil Jørgen , Svendsen Jesper Hastrup
Published: February 2024

Background

The ABC‐stroke score is a risk scheme for prediction of stroke or systemic embolism (SE) in atrial fibrillation (AF). This study sought to examine whether the score could be useful in predicting stroke in AF‐naïve individuals and risk stratifying for AF screening.

Methods and Results

The LOOP (Atrial Fibrillation Detected by Continuous ECG Monitoring Using Implantable Loop Recorder to Prevent Stroke in High‐Risk Individuals) study randomized 6004 AF‐naïve individuals aged 70 to 90 years with stroke risk factors to either screening with an implantable loop recorder and anticoagulation upon detection of new‐onset AF episodes ≥6 minutes, or usual care. A total of 5781 participants had available ABC‐stroke score at baseline and were included in this secondary analysis: 4170 (72.1%) with an estimated stroke/SE risk ≤1%/year versus 1611 (27.9%) with an estimated stroke/SE risk >1%/year. Having an annual ABC‐stroke risk >1% was associated with stroke/SE, stroke/SE/cardiovascular death, and all‐cause death (hazard ratio, 1.82 [95% CI, 1.44–2.21], 2.17 [95% CI, 1.80–2.62], and 2.19 [95% CI, 1.87–2.56], respectively). For screening with implantable loop recorder versus usual care, no significant reduction in these study outcomes was obtained in any ABC‐stroke risk groups (
P
>0.0500 for all), with no signal toward interaction (
P
interaction
>0.2500 for all). Similar findings were yielded when assessing the ABC‐stroke score as a continuous variable.

Conclusions

In an elderly, AF‐naïve population with additional stroke risk factors, a higher ABC‐stroke score could identify individuals with increased stroke risk. However, this risk score may not be useful in pinpointing those more likely to benefit from AF screening and subsequent preventive treatment. These findings should be considered as hypothesis generating and warrant further study.

Registration

URL:
https://www.clinicaltrials.gov
; unique identifier: NCT02036450.
Role of Cognitive Frailty in Older Adults With Cardiovascular Disease
Authors: Ijaz Naila , Jamil Yasser , Brown Charles H. , Krishnaswami Ashok , Orkaby Ariela , Stimmel Marnina B. , Gerstenblith Gary , Nanna Michael G. , Damluji Abdulla A.
Published: February 2024

As the older adult population expands, an increasing number of patients affected by geriatric syndromes are seen by cardiovascular clinicians. One such syndrome that has been associated with poor outcomes is cognitive frailty: the simultaneous presence of cognitive impairment, without evidence of dementia, and physical frailty, which results in decreased cognitive reserve. Driven by common pathophysiologic underpinnings (eg, inflammation and neurohormonal dysregulation), cardiovascular disease, cognitive impairment, and frailty also share the following risk factors: hypertension, diabetes, obesity, sedentary behavior, and tobacco use. Cardiovascular disease has been associated with the onset and progression of cognitive frailty, which may be reversible in early stages, making it essential for clinicians to diagnose the condition in a timely manner and prescribe appropriate interventions. Additional research is required to elucidate the mechanisms underlying the development of cognitive frailty, establish preventive and therapeutic strategies to address the needs of older patients with cardiovascular disease at risk for cognitive frailty, and ultimately facilitate targeted intervention studies.
LncRNA AC003090.1 Mediates miR-203a-3p/FOXP1 Axis to Promote the Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells
Authors: Zhuang Huafeng , Yao Xuedong , Wang Aifei , Li Junjie , Zheng Miao , Dong Yu , Xu Youjia , Li Yizhong , Lin Yongjun
Published: February 2024

We investigated the regulatory mechanism of lncRNA AC003090.1 in human bone marrow stem cells (hBMSCs). Tissues from patients with osteoporosis (OP) were collected for the detection and analysis of AC003090.1, miR-203a-3p, and FOXP1 expression by QPCR. The expression and activity of
alkaline phosphatase (ALP), an osteogenic marker, were detected using a modified Gomori calcium-cobalt assay and PNP colorimetric assay. Calcium deposition on the extracellular surface was demonstrated using alizarin red staining. Using the Oil Red O staining assay to detecte the Intracellular
lipid content. Dual luciferase reporting system verified the targeting relationship between AC003090.1 or FOXP1 and miR-203a-3p. qRT-PCR and Western blot were used to measure the expression level of β-catenin in hBMSCs after different intervention treatments. The expression levels
of AC003090.1 and FOXP1 were downregulated in Osteoporosis (OP), whereas those of miR-203a-3p were upregulated. An increase in AC003090.1 expression could enhance hBMSC osteogenic differentiation (OD) and reduce the adipogenic ability of hBMSCs. Furthermore, miR-203a-3p mimics or FOXP1 knock-down
reversed the effect of increased AC003090.1 expression on OD and adipogenic differentiation of hBMSCs. Dual luciferase reporter assays showed that AC003090.1 can sponge miR-203a-3p, which targets FOXP1 in hBMSCs. LncRNA AC003090.1 promotes OD of hBMSCs by regulating the miR-203a-3p/FOXP1 axis.
Follistatin-Like Protein 1 Alleviates Renal Ischemia-Reperfusion Injury by Regulating MicroRNA-21
Authors: Lin Guoxiong , Chai Shiquan , Mei Kaibo , Xiong Guixiang , Liu Fanglan , Mao Haifei
Published: February 2024

A mouse renal ischemia-reperfusion injury (RIRI) model was used to investigate how follistatin-Like Protein 1 (FSTL1) provides renal protection post-RIRI by targeting inflammation, apoptosis, and microRNA (miRNA). RIRI was induced in 8-week-old male C57BL/6 mice, followed by FSTL1 recombinant
protein treatment. Inflammation and apoptosis in kidney tissues were assessed using ELISA and flow cytometry. A cellular RIRI model was created using hypoxia/reoxygenation (H/R) in HK-2 cells to validate FSTL1’s effects. miRNA-mediated mechanisms were explored using cell transfection
and dual-luciferase assays. RIRI mice exhibited elevated inflammation and apoptosis, while FSTL1 treatment mitigated these effects. Similarly, FSTL1 attenuated H/R-induced HK-2 cell damage. miR-21 expression decreased in H/R-treated HK-2 cells, which FSTL1 reversed. miR-21 mimic reduced H/R-induced
HK-2 cell damage, while its inhibition decreased FSTL1’s protection. Notably, miR-21 targeted caspase-7 and suppressed its activity. FSTL1 alleviated mouse RIRI by upregulating miR-21, thereby reducing inflammation and apoptosis in kidney tissues post-RIRI. This study highlights FSTL1’s
therapeutic potential through the miR-21-mediated regulation of inflammation and apoptosis in RIRI.
Blocking Runx2 Inhibits the Caspase-1 Dependent Pyroptosis in Lipopolysaccharide-Treated Chondrocyte
Authors: Yuan Libo , Yao Ling , Ren Xianzhen , Chen Xusheng , Kang Kaiqiang , Xu Yongqing , Jin Tao
Published: February 2024

Pyroptosis is a new type of cell death in the development of osteoarthritis (OA), but the underlying mechanism is not fully understood. This study aimed to investigate the role of Runx2 in the pyroptosis of chondrocyte (CH) and explore its effect on Caspase-1 expression. Human knee
tissues from the fracture patients without OA history were collected. Human CHs isolated from the tissue were treated by lipopolysaccharide (LPS) to establish the model of OA. siRNA and CADD522 were used to block the function of Runx2. The cell viability was tested by MTT. The expression levels
of Runx2, Caspase1/4/5/11, GSDMD, collagen-II, aggrecan, IL-1β, IL-8, and MMP3/9 were detected by RT-PCR, immunofluorescence, or western blot. Besides, the transcriptional association between Runx2 and Caspase-1 was confirmed by DNA immunoprecipitation and luciferase reporter assay.
Runx2 and Caspase-1 expression were increased in LPS-treated CHs. Runx2 bound to the promoter of Caspase-1 and activated its expression. Moreover, silencing Runx2 or disrupting the DNA-binding ability of Runx2 attenuated the LPS-induced pyroptotic phenotype, containing Caspase-1 activation,
collagen-II and aggrecan degradation, viability suppression, IL-1β and IL-8 upregulation. Blocking the expression or function of Runx2 alleviated the LPS-caused pyroptosis in CHs in the Caspase-1 manner, indicating a novel understanding of the pathology of OA.
Study on the Mechanism and Protection of Salt-Sensitive Hypertensive Rats’ Myocardial Fibrosis by Regulating Striatin with Sacubatrovalsartan
Authors: Tu Qingxian , Xia Qianhang , Chen Meihong , Zhou Haiyan , Jiang Qianfeng , Li Wei
Published: February 2024

This study aims to explore the relationship between STRN, TGF-β1, Caspase-3, PD-1 expression, and myocardial fibrosis in salt-sensitive hypertensive rats. It investigates the correlation between STRN expression and myocardial fibrosis, along with the protective effects of
Sacubitril/Valsartan (ARNI). Fifteen 18-week-old rats were divided into three groups: Control, high salt (SSH), and ARNI+SSH. Blood pressure was monitored weekly for 8 weeks. Echocardiography evaluated cardiac parameters, while H&E and Masson staining visualized myocardial morphology and
fibrosis. Immunohistochemistry measured protein expression of collagen-1, collagen-3, TGF-β1, PD-1, Caspase-3, and STRN. Western blot assessed STRN protein levels. High-salt diet increased fibrosis, collagen expression, TGF-β1, PD-1, Caspase-3, and reduced STRN expression
compared to Control (P < 0.05). ARNI treatment decreased fibrosis, collagen expression, TGF-β1, PD-1, Caspase-3 (P <0.05), and increased STRN expression compared to SSH (P <0.05). STRN expression correlated positively with myocardial fibrosis. ARNI
demonstrated potential in attenuating fibrosis by modulating STRN expression and suppressing apoptosis and inflammation in salt-sensitive hypertensive rats.
Hyper-Methylation of CpG Island in 5′ UTR of the HLA-G Gene Reduces Its Expression in Individuals with Immune Thrombocytopenia
Authors: Ma Ji , Ning Yunna , Lu Ke , Wang Hui , Li Ping , Feng Lili , Zhang Jianing , Xie Linna , He Qiang
Published: February 2024

This study investigated the impact of DNA methylation in the 5′ untranslated region-CpG island (5′ UTR) of the HLA-G gene on soluble HLA-G (sHLA-G) levels in immune thrombocytopenia (ITP) patients, shedding light on sHLA-G’s regulatory mechanisms in ITP. Using a cohort
of 53 participants, including ITP patients, DNA methylation profiles in the HLA-G gene’s 5′ UTR were analyzed with Sequenom MassARRAY Methylation Analysis. sHLA-G levels were measured by enzyme-linked immunosorbent assay, and platelet antibodies were assessed using modified MAIPA.
Results showed increased DNA methylation at specific CpG sites (CpG3, CpG18, CpG19, and CpG20.21) in ITP patients. A negative correlation between DNA methylation and sHLA-G expression, particularly at CpG18, was found. Patients with Anti-GPIb/IX antibodies had higher CpG18 methylation. Age
and gender didn’t correlate significantly with methylation. This underscores 5′ UTR hypermethylation’s role in influencing circulating HLA-G levels, revealing insights into ITP development and potential therapeutic targets. By linking DNA methylation to sHLA-G expression,
this advances ITP understanding, suggesting new therapeutic strategies.
The Impact of LncRNA Nuclear-Enriched Abundant Transcript 1-Mediated Regulation of ASK1 Expression via miR-20a on High Glucose-Induced Retinal Vascular Endothelial Cell Injury
Authors: Zhao Ling , Cai Chunhua , Yi Congjian , Liu Chaobin
Published: February 2024

This study investigates how long non-coding RNA (LncRNA) NEAT1 influences high glucose-induced damage in human retinal vascular endothelial cells (hRECs). Different experimental groups were established, including normal, high glucose, LncRNA NEAT1 knockdown, and miR-20a inhibition.
Assessments were conducted for molecular and functional changes. In high glucose conditions, NEAT1 expression increased while miR-20a expression decreased in hRECs. Silencing NEAT1 reduced its levels and increased miR-20a expression. Consequently, reactive oxygen species (ROS), MDA, 4-HNE,
IL-1β, TNF-α, ICAM-1, ASK1, VEGF, and p-p38 MAPK/p38 MAPK ratio decreased. This led to diminished cell proliferation, migration, and tube formation in hRECs. The impact of NEAT1 silencing was partially reversed by miR-20a inhibition, suggesting NEAT1′s regulatory
role via miR-20a. NEAT1 suppressed miR-20a and ASK1 protein levels. Additionally, LncRNA NEAT1 sequestered miR-20a, contributing to ASK1 downregulation. This process also suppressed p38 MAPK activation, further inhibiting hREC functions. In summary, NEAT1 modulated high glucose-induced hREC
injury by downregulating miR-20a and subsequently impacting ASK1 and p38 MAPK pathways, thereby impairing cell functions. This study provides insights into potential therapeutic targets for diabetic retinopathy.
The Value of Near-Infrared Multifunctional Nanoprobe Combined with Artificial Intelligence Microsensor Technology in Molecular Diagnosis for Gastric Cancer
Authors: Sun Qiang , Yao Jun , Wei Shuxun , Li Xinxing , Wang Weijun
Published: February 2024

Since the symptoms of early gastric cancer patients are not obvious, the majority of new gastric cancer cases are progressive gastric cancer every year. In this paper, we applied nanomedicine technology to design and prepare multifunctional nanoparticles for the diagnosis and treatment
of gastric cancer. Through targeted imaging of gastric cancer, combined with phototherapy and the prepared nanoprobes are applied to the ectopic transplantation tumor model of gastric cancer. Meanwhile, a fluorescent microsensor based on graphene oxide and deoxyribonuclease is constructed
in order to realize the rapid detection of gastric cancer exosomes. The near-infrared multifunctional nanoprobe is combined with artificial intelligence microsensor technology and applied to the molecular diagnosis of gastric cancer. The results shows that the P-P-I-M+ laser irradiation group
has the highest fluorescence intensity and its average fluorescence intensity is 2.04 times higher than that of the P-P-I+ laser irradiation group. The relative cell viability of P-P-M+ laser irradiation group, P-P-I+ laser irradiation group and P-P-I-M+ laser irradiation group are 62.5%,
41.9% and 19.3%, respectively. Therefore, the method in this paper can reduce the non-specific toxicity to other organs as well as exert the effect of combining the diagnosis and treatment of gastric cancer.
Upregulation of LncRNA-LHX2 Promotes Hepatocellular Carcinoma Proliferation, Migration, Invasion and Metastasis via Targeting VEGFA by Sequestering of MiR-939-5p
Authors: Fang Caiyue , Lin Ruibo , Gan Suqin , Wang Hong , Huang Chenghui
Published: February 2024

Due to the challenges in early diagnosis and lack of specific biomarkers, liver cancer remains one of the most prevalent and lethal tumor types. Numerous studies have shown that long noncoding RNA (lncRNA) plays a crucial role in the regulation of various malignant tumors, including
liver cancer. Here, we discussed the function and effect of LncRNA-LHX2 in the tumorigenesis and progression of liver cancer, which was significantly upregulated in liver cancer tissues, compared to the benign liver tissues. To improve the accuracy and efficiency of tests like qRT-PCR, we
employed nano-magnetic beads for nucleic acid extraction from tissues and cells. In our experiments using HepG2 cells, silencing of LncRNA-LHX2 effectively suppressed cell proliferation, migration, and invasion by interacting with miR-939-5p, which targets VEGFA. Interestingly, overexpression
of miR-939-5p also impaired malignant functions of HepG2 cells. However, simultaneously inhibition of miR-939-5p expression can partially restored the inhibitory effect on HepG2 cells resulting from LncRNA-LHX2 knockdown. Consistently, our in vivo results from tumor mice model also
suggested that knockout of LncRNA-LHX2 inhibited the tumor growth and suppressed epithelial mesenchymal transition (EMT) process, while silencing of miR-939-5p exhibited the opposite effect. However, when both LncRNA-LHX2 and miR-939-5p were simultaneously interfered with, the tumor growth
was partially alleviated. Based on these results, our study highlights the malignant impact of LncRNA-LHX2 in the progression of liver cancer, indicating its potential as a candidate biomarker for liver cancer diagnosis.
Inhibition of HaCaT Proliferation and Imiquimod-Induced Psoriasis by Calcipotriol Through Regulation of the Glutathione/Glutathione Peroxidase 4 Pathway
Authors: Yang Lei , Zhang Yue , Wu Jiansong , Wang Lei , Liu Shan , Zhou Li , Zhang Jigang , Li Chengxin
Published: February 2024

Psoriasis is a chronic and recurrent skin disease characterized by aberrant proliferation and differentiation of keratinocyte cells. Although calcipotriol has been employed in the clinical management of psoriasis, no association between the anti-inflammatory mechanism and iron death
has been reported. Therefore, we assume that calcipotriol may down-regulate cell activity and suppress the expression of tissue inflammatory factors by regulating the glutathione (GSH) and glutathione peroxidase 4 (GPX4) pathway, thereby alleviating tissue inflammation and ameliorating psoriasis
symptoms. The experimental groups consisted of a control group, a model group, a Calcipotriol group, and a Calcipotriol+Ferrostatin-1 group. In vitro experiments, a lipopolysaccharides-induced HaCaT cell model was established. In vivo experiments, an imiquimod-induced psoriasis
mice model was constructed. The results showed that calcipotriol effectively downregulated the expression of GPX4 and GSH, thereby inhibiting HaCaT cell proliferation through modulation of Ki-67 protein expression and DNA breakage. Ferrostatin-1 could partially reverse these effects. Additionally,
calcipotriol downregulated the expression of GPX4 and GSH in skin tissues and upregulated the expression of long-chain acyl-CoA synthetase 4 by suppressing the levels of SLC7A11 and ferritin, leading to promote the accumulation of ROS and ferroptosis. Moreover, calcipotriol demonstrated inhibitory
effects on the inflammatory mediators and attenuated skin inflammation. Therefore, calcipotriol effectively ameliorated psoriatic lesions. In conclusion, this study revealed that calcipotriol exerts its therapeutic potential by promoting cellular clearance and suppressing tissue inflammation
through upregulation of ferroptosis progression. Therefore, this study provides new therapeutic drugs and functions for the treatment of psoriasis.
The Mechanism of miR-223 Inhibiting Treg Differentiation in Autoimmune Encephalomyelitis by Targeting Forkhead box-O3-Forkhead box-P3 Using Nano-Albumin Particles as a Carrier
Authors: Tan Ziming , Wang Jun , Zhang Mei , Zhu Hongtao , Luo Qiong
Published: February 2024

Central nervous system (CNS) inflammatory demyelinating diseases target oligodendrocytes or supporting cells. Millions of patients worldwide suffer from a variety of symptoms including vision. Motor and sensory impairments are increasingly recognized in children, but treatments for
these disorders remain very limited. This study intends to assess the mechanism of miR-223 in inhibiting regulatory T cells differentiation in autoimmune encephalomyelitis. Electron microscopy revealed nanoparticle size. miR-223 expression in tissues was detected by mRNA. MTT method and flow
cytometry were used to detect Treg cell activity, proliferation and differentiation. We further studied the mechanism by which miR-223 inhibits Treg differentiation and experimental autoimmune encephalomyelitis by targeting FOXO3-FOXP3. Animal experiments were conducted on the therapeutic
potential of miR-223 antagonists to confirm whether miR-223 antagonists have an effect on Experimental allergic encephalomyelitis (EAE). In our previous studies, we found that the expression of miR-223 was up-regulated in EAE and children with MOG antibody-related demyelinating diseases. Through
bioinformatics analysis, we found that FOXO3 could be used as a target gene of miR-223. FOXO3 is targeted by miR-223. Using nano-albumin particles as the carrier, miR-223 mimic reduced cell activity while FOXP3 overexpression partially offset the inhibitory effect. Overexpression of FOXP3
restored the Treg induced by using nano-albumin particles as the carrier of miR-223 mimic. Our study shows that nano-albumin particles carrying miR-223 can inhibit Treg cell differentiation by targeting FOXO3. This study provides a theoretical basis for clinical research.
Liposome Nanoparticles Loaded with Paeoniflorin Protect Neuronal Damage in Parkinson’s Disease by Regulating miR-135a
Authors: Wang Pin , Xia Dongxia , Wang Yihe , Qu Yue
Published: February 2024

Paeoniflorin (PAE) is an active ingredient extracted from peony. This study aimed to explore the mechanism by which liposome nanoparticles loaded with PAE protect neuronal damage in Parkinson’s disease. Model group, PAE group, PAE-Lips group, PAE-Lips+miR-135a agonist group, PAE-Lips+miR-135a
inhibitor group, PAE-Lips+BAY11-7085 group, PAE-Lips+SC75741 group were designed. PCR, learning and memory ability testing, pole climbing test, etc. were used to determine the mechanism of PAE-Lips on Parkinson’s disease and whether it exerts effects through regulating miR-135a. PAE-Lips
were successfully constructed. PAE-Lips improved Parkinson’s disease in rats and had a certain connection with miR-135a. Up-regulating miR-135a inhibited NF-κB pathway to a certain extent and improved Parkinson’s disease. It helped protect neurons. Further verification
using PAE-Lips+miR-135a agonists, SC75741, BAY11-7085, etc. showed that PAE-Lips upregulated the expression of miR-135 and inhibited NF-κB pathway, which has a good protective effect on neurons in Parkinson’s disease. PAE-Lips can promote miR-135a to inhibit the NF-κB
pathway, thereby protecting neuronal damage in Parkinson’s disease. This study will provide a new idea for the prevention and treatment of Parkinson’s disease, clarify the impact of PAE-Lips, miR-135a, NF-κB, BAY11-7085 and SC75741 on Parkinson’s disease, and
provide a basis for the combined use of these interventions. The possibility of treating Parkinson’s disease more effectively deserves further exploration and research and provides a theoretical basis for the development of related therapeutic drugs.
A Simvastatin-Loaded Nanoliposome Delivery System for Sepsis-Induced Acute Lung Injury
Authors: Yang Jianhai , Yue Yue
Published: February 2024

To enhance the treatment of acute lung injury (ALI) induced by sepsis and optimize the clinical efficacy of simvastatin (SV), we develop SV-loaded nanoliposomes (SV/NLC) as a novel drug delivery system. The NLCs exhibited a particle size of approximately 165 nm, which increased to around
195 nm upon SV loading. NLCs significantly prolonged the half-life of SV by nearly five-fold and improved its penetration into EA.hy926 cells, demonstrating excellent biocompatibility and targeted delivery for ALI therapy. In the rat model of ALI, the SV/NLC effectively reduced the lung wet/dry
ratio and the levels of inflammatory factor and albumin in the alveoli, thus improving the alveolar gas exchange function and blood oxygenation. The SV/NLC group demonstrated superior suppression of oxidative stress within lung tissues compared to other groups. Notably, treatment with SV reduction
in TLR4, MyD88, and NF-κB P65 levels in lung tissues from ALI rat models. This effect was particularly pronounced in the SV/NLC group. Furthermore, SV can effectively mitigate inflammatory responses and oxidative stress in ALI treatment by modulating the TLR4/NF-κB
signaling pathway. In conclusion, our findings suggest that SV can exert therapeutic effects against sepsis-induced ALI through inhibition of the TLR4/NF-κ and mitigate inflammatory response and oxidative stress.
Accuracy of 3D-Printed and Digitally Fabricated Polymeric Models for Dental Applications: A Systematic Review and Meta-Analysis
Authors: Alshahrani Abdullah
Published: February 2024

Objective: To date, no systematic review has reported the accuracy of conventional gypsum casts compared to 3D printed and digital models. The aim of this review is to critically appraise the studies that have focused on comparing the accuracy and fit of casts produced with 3D
printing. Methods: Electronic literature databases (PubMED, ISI Web of Knowledge, EMBASE, Scopus, ClinicalTrials. gov and CENTRAL) were searched by operators specializing in health sciences databases using relevant keywords. The focused question was, “For the treatment of missing
teeth (Participants), do the 3D-printed or digital casts (Intervention) have superior accuracy (Outcomes), compared to conventional gypsum casts (Control)? Meta-analysis was conducted and Risk of bias was assessed using RoB 2.0 and QUIN tools. Results: 1770 items were selected, after
removal of 1520 articles, abstracts and titles of 250 items were read for potential eligibility. Fourteen studies were included in this review. 3-D printed casts resulted in production of prostheses with similar accuracy to gypsum-based products. The accuracy of completely digitally processed
casts was debatable. Sources of bias were found in the risk of bias assessment. Conclusions: It is concluded that the accuracy of 3D-printed casts are comparable to those of conventional gypsum cast. In addition to cast accuracy, low technique sensitivity, operator independence and
cost and time efficiency makes 3D printed casts an effective alternative for conventional and digital cast in dental laboratory procedures.
LINC00460/miR-143-3p/Serpine1 in Promoting Gastric Cancer (GC) Progression
Authors: Li Wulong , Yu Haitao , Wei Dan , Ouyang Gen , Li Ying , Tu Qing
Published: February 2024

Aim: Our research was to evaluate lncRNA LINC00460 in gastric cancer development and relative mechanisms by vitro study. Materials and methods: Using RT-qPCR assay to measure LINC00460, miR-143-3p, Serpine1, Vimentin, N-cadherin and E-cadherin mRNA, Serpine1, Vimentin,
Ncadherin and E-cadherin expression evaluated using WB assay. Using Edu and CCK-8 assay to evaluate cell proliferation, cell apoptosis rate were evaluated by flow cytometry. Invasion cell number and wound healing rate were measured by transwell and wound healing assay. Analysis miR-143-3p
and Seprine 1 correlation using Dual Luciferase Reporter. Results: With LINC00460 knockdown, gastric cancer cells’ proliferation were significantly depressed with apoptosis significantly increasing (P < 0.001, respectively). Meanwhile, gastric cancer cells invasion and
migration abilities were significantly depressed (P < 0.001, respectively) and miR-143-3p and E-cadherin expression were significantly up-regulation, and Serpine 1, Vimentin and N-cadherin gene and proteins expression were significantly down-regulation (P < 0.001, respectively).
However, miR-143-3p inhibitor supplement, GC cells’ biological activities were significantly enhanced (P < 0.001, respectively) with relative gene and proteins expressions were significantly changed (P < 0.001, respectively). By Dual Luciferase Reporter, miR-143-3p
could target Serpine 1. Conclusion: lncRNA LINC00460 knockdown could depress gastric cancer cell biological activities by regulation miR-143-3p/Serpine1 axis in vivo study.
Enhancement of Antioxidant/Anti-Inflammatory Activity, and Acceleration of Wound Healing Through the Combination of Ginger Extract and NAC: A Promising Approach
Authors: Zhou Conglai , Zhao Kaixu , You XiaoLi , Wang Yuanzheng , Chen Long , Sun Li
Published: February 2024

In recent years, there has been significant development and application of bioactive materials with antioxidant and anti-inflammatory properties in skin regenerative medicine. In this study, we utilized sodium hyaluronate (HA), which has good biological activity and compatibility, as
a carrier for material mixing. Ginger extract (GE), a natural biologically active compound, was combined with N-Acetyl-L-cysteine (NAC) for the treatment of skin wounds. GE was extracted from fresh ginger juice through freeze-drying, which exhibit pharmacological effects such as antioxidant,
anti-inflammatory, and antibacterial properties. Additionally, NAC possesses notable pharmacological activities, particularly as an antioxidant and anti-inflammatory agent. By harnessing the natural advantages of GE in combination with NAC for wound healing, remarkable results were observed
in terms of antioxidant efficacy, anti-inflammatory effects, promotion of granulation tissue and wound repair processes. These outcomes surpassed those achieved by using the individual components alone. These experimental findings underscore the potential of combining plant-based medicines
and chemical drugs. This approach strengthens the synergistic effects of drug efficacy, reduces the use of and side effects associated with chemical drugs, and holds significant research and development potential. Moreover, it promotes the wider adoption and application of traditional Chinese
medicine technology.
Biomaterials for Peripheral Nerve Injury Repair
Authors: Wu Jiarui , Guo Hao , Chen Long , Wang Yuanzheng , Sun Li
Published: February 2024

Peripheral nerve injury (PNI) is a common condition in orthopedic clinical practice, leading to sensory and motor dysfunction in the affected limbs. The quality of life for patients is drastically affected by this, causing a significant burden on their family and society. Currently,
patients with peripheral nerve transection injuries or combined nerve defects requiring autologous nerve transplantation undergo surgical treatment. However, postoperative functional recovery of the affected limbs is often incomplete, and the source of autologous nerve grafts is limited. Therefore,
promoting damaged nerve repair and restoration of limb function remains a challenging issue. In recent years, with the continuous advancement of materials science, tissue engineering, and regenerative medicine, the development of biomaterials has provided a new approach for repairing PNI.
Biomaterials used for PNI repair include polymer materials, natural materials, and composite materials, with many experimental research results indicating their ability to promote the repair of PNI. This article reviews the application of biomaterials in the repair of PNI, discussing their
unique properties and advantages and disadvantages for peripheral nerve restoration. The aim is to provide theoretical support for the further development of novel biomaterials for PNI repair.
Superresolution of GOES-16 ABI Bands to a Common High Resolution with a Convolutional Neural Network
Authors: White Charles H. , Ebert-Uphoff Imme , Haynes John M. , Noh Yoo-Jeong
Published: February 2024

Superresolution is the general task of artificially increasing the spatial resolution of an image. The recent surge in machine learning (ML) research has yielded many promising ML-based approaches for performing single-image superresolution including applications to satellite remote sensing. We develop a convolutional neural network (CNN) to superresolve the 1- and 2-km bands on the GOES-R series Advanced Baseline Imager (ABI) to a common high resolution of 0.5 km. Access to 0.5-km imagery from ABI band 2 enables the CNN to realistically sharpen lower-resolution bands without significant blurring. We first train the CNN on a proxy task, which allows us to only use ABI imagery, namely, degrading the resolution of ABI bands and training the CNN to restore the original imagery. Comparisons at reduced resolution and at full resolution with Landsat-8/Landsat-9 observations illustrate that the CNN produces images with realistic high-frequency detail that is not present in a bicubic interpolation baseline. Estimating all ABI bands at 0.5-km resolution allows for more easily combining information across bands without reconciling differences in spatial resolution. However, more analysis is needed to determine impacts on derived products or multispectral imagery that use superresolved bands. This approach is extensible to other remote sensing instruments that have bands with different spatial resolutions and requires only a small amount of data and knowledge of each channel’s modulation transfer function.

Significance Statement

Satellite remote sensing instruments often have bands with different spatial resolutions. This work shows that we can artificially increase the resolution of some lower-resolution bands by taking advantage of the texture of higher-resolution bands on the GOES-16 ABI instrument using a convolutional neural network. This may help reconcile differences in spatial resolution when combining information across bands, but future analysis is needed to precisely determine impacts on derived products that might use superresolved bands.