bluuebunny/crossref_metadata_2025 huggingface dataset:
A flyway perspective of foraging activity in Eurasian Green-winged Teal,Anas crecca crecca
Authors: Arzel C. , Elmberg J. , Guillemain M.
Published: May 2007

Time–activity budgets in the family Anatidae are available for the wintering and breeding periods. We present the first flyway-level study of foraging time in a long-distance migrant, the Eurasian Green-winged Teal, Anas crecca crecca L., 1758 (“Teal”). Behavioral data from early and late spring staging, breeding, and molting sites were collected with standardized protocols to explore differences between the sexes, seasons, and diel patterns. Teal foraging activity was compared with that of the Mallard, Anas platyrhynchos L., 1758 and Northern Shoveler, Anas clypeata L., 1758, and the potential effects of duck density and predator-caused disturbance were explored. In early spring, foraging time was moderate (50.5%) and mostly nocturnal (45%). It increased dramatically in all three species at migration stopovers and during molt, mostly because of increased diurnal foraging, while nocturnal foraging remained fairly constant along the flyway. These patterns adhere to the “income breeding” strategy expected for this species. No differences between the sexes were recorded in either species studied. Teal foraging time was positively correlated with density of Teal and all ducks present, but negatively correlated with predator disturbance. Our study suggests that Teal, in addition to being income breeders, may also be considered as income migrants; they find the energy necessary to migrate at staging sites along the flyway.
Isotopic (δ15N and δ13C) evidence for intersexual foraging differences and temporal variation in habitat use in waved albatrosses
Authors: Awkerman Jill A. , Hobson Keith A. , Anderson David J.
Published: May 2007

Waved albatrosses ( Phoebastria irrorata Salvin, 1883) forage close to their breeding grounds on Isla Española, Galápagos, during the short chick-brooding stage and make long trips to the Peruvian upwelling during incubation and chick rearing. Previous studies have suggested foraging segregation by sex: females spend more time searching than males do while foraging in the Galápagos Marine Reserve (GMR), and band recoveries suggest higher bycatch vulnerability of males in the Peruvian upwelling. We used stable isotope analysis (δ15N and δ13C) of whole blood of adult male and female albatrosses to test for intraspecific foraging segregation in this sexually dimorphic species. Analysis of serial blood samples revealed higher δ15N values in males, suggesting consumption of prey items of higher trophic level. We also detected seasonal variation in foraging ecology, with higher δ15N values at the beginning of the breeding season. Sex and regional differences in δ13C values were not significant, reflecting primarily pelagic foraging sites of both sexes, both in the GMR and the Peruvian upwelling. Our results provide evidence of trophic segregation, suggestive of competitive exclusion, and novel information on marine isoscape values in the Eastern Equatorial Pacific.
Assessing western gull predation on purple sea urchins in the rocky intertidal using optimal foraging theory
Authors: Snellen C.L. , Hodum P.J. , Fernández-Juricic E.
Published: May 2007

Purple sea urchins ( Strongylocentrotus purpuratus (Stimpson, 1857)) are abundant grazing invertebrates that can have a major impact on the rocky intertidal community. Predators can control the urchin population and indirectly reduce grazing activity. We determined the effects of western gull ( Larus occidentalis Audubon, 1839) predation on purple sea urchins in the rocky intertidal using the framework of optimal foraging theory and taking into account different prey-handling techniques. We recorded the foraging behavior of gulls, measured urchin availability, and estimated prey caloric content with bomb calorimetry. Western gulls selected purple sea urchins significantly more than other prey items (snails (genus Tegula Lesson, 1835), limpets (genus Collisella Dall, 1871), sea stars ( Pisaster giganteus (Stimpson, 1857) and Pisaster ochraceus (Brandt, 1835))). Larger urchins contained relatively more calories. Gulls foraged optimally when pecking by frequently selecting the most profitable size class. However, gulls chose smaller urchins than expected when air-dropping, which could have been influenced by group size and age. Gulls selected smaller purple sea urchins when foraging in larger groups likely owing to the risk of kleptoparasitism. Adults chose larger, and juveniles smaller, urchins when air-dropping, suggesting that juveniles are less experienced in foraging techniques. We estimated that gull predation could affect up to one third of the sea urchin populations locally, which could increase species diversity in the rocky intertidal community.
Dispersal, survival, and reproduction of wild-born, yearling swift foxes in a reintroduced population
Authors: Ausband D.E. , Foresman K.R.
Published: May 2007

From 1998 to 2002, the Blackfeet Tribe and Defenders of Wildlife reintroduced 123 captive-reared swift foxes ( Vulpes velox (Say, 1823)) to the Blackfeet Reservation in northern Montana. Because yearling swift foxes are colonizers of vacant habitats and are at the periphery of range expansion, we estimated 1st-year survival and explored aspects of dispersal to ascertain the contribution of wild-born juveniles to the reintroduction effort. First-year survival (post den emergence) of swift foxes averaged 0.38 and 0.36 for 2003–2004 and 2004–2005, respectively. Half of the females that survived to 1 June of their 1st year reproduced. Most dispersal (77%) and mortality (85%) occurred in autumn, with 80% of mortalities attributable to predation. Dispersal distance was not different among sexes and averaged 10.4 km. Understanding the contribution of yearling swift foxes — the colonizers — to reestablishing populations is important because several entities are currently reintroducing swift foxes and some states have expanding populations.
Ecological and genetic evidence that low-order streams inhibit dispersal by red-backed salamanders (Plethodon cinereus)
Authors: Marsh D.M. , Page R.B. , Hanlon T.J. , Bareke H. , Corritone R. , Jetter N. , Beckman N.G. , Gardner K. , Seifert D.E. , Cabe P.R.
Published: May 2007

While many studies have examined the barrier effects of large rivers on animal dispersal and gene flow, few studies have considered the barrier effects of small streams. We used displacement experiments and analyses of genetic population structure to examine the effects of first-order and second-order streams on the dispersal of terrestrial red-backed salamanders, Plethodon cinereus (Green, 1818). We marked red-backed salamanders from near the edges of one first-order stream and one second-order stream, and experimentally displaced them either across the stream or an equal distance farther into the forest. A comparison of return rates indicated that both streams were partial barriers to salamander movement, reducing return rates by approximately 50%. Analysis of six microsatellite loci from paired plots on the same side and on opposite sides of the second-order stream suggested that the stream did contribute to genetic differentiation of salamander populations. Collectively, our results imply that low-order streams do influence patterns of movement and gene flow in red-backed salamanders. We suggest that given the high density of first-order and second-order streams in most landscapes, these features may have important effects on species that, like red-backed salamanders, have limited dispersal and large geographic ranges.
Do parasites affect burrowing activity and emergence of sand hoppers, Talorchestia quoyana (Amphipoda: Talitridae)?
Authors: Currey Rohan J.C. , Poulin Robert
Published: May 2007

Sand hoppers (Amphipoda: Talitridae) are semiterrestrial crustaceans that feed upon stranded kelp. Their burrowing behaviour plays an important role in reintroducing nutrients into the sediment. The most abundant sand hopper on New Zealand’s beaches is Talorchestia quoyana Milne-Edwards, 1840. It is host to a parasitic mermithid nematode, Thaumamermis zealandica Poinar, Latham and Poulin, 2002, which invariably kills its sand hopper host by emerging to complete its maturation and reproduction in a moist sand environment. The aim of the present study was to assess if the burrowing behaviour of Ta. quoyana showed pathologic consequences of infection by Th. zealandica. Two experiments were conducted to assess temporal variation in sand hopper burrowing in vitro. Parameters measured included the hour after sunrise and sunset that sand hoppers first emerged and the number of surface visits in the first hour after emergence. Across experiments, sand hopper burrowing behaviour showed considerable heterogeneity related to sand hopper length, sand hopper distribution, experimental series, and experimental moisture conditions. The presence of parasites was not a significant factor in determining sand hopper burrowing behaviour. The lack of pathological effect is surprising given the relative size of Th. zealandica.
RENORMALIZATION GROUP AND GRAND UNIFICATION WITH 331 MODELS
Authors: DIAZ RODOLFO A. , GALLEGO D. , MARTINEZ R.
Published: May 2007

By making a renormalization group analysis we explore the possibility of having a 331 model as the only intermediate gauge group between the standard model and the scale of unification of the three coupling constants. We shall assume that necessarily there is no group of grand unification at the scale of convergence of the couplings. With this scenario, different 331 models and their corresponding supersymmetric versions are considered, and we find the versions that allow the symmetry breaking described above. Besides, the allowed interval for the 331 symmetry breaking scale, and the behavior of the running coupling constants are obtained. It is worth saying that some of the supersymmetric scenarios could be natural frameworks for split supersymmetry. Finally, we look for possible 331 models with a simple group at the grand unification scale that could fit the symmetry breaking scheme described above.
SCHRÖDINGER SECANT LOWER BOUNDS TO SEMIRELATIVISTIC EIGENVALUES
Authors: HALL RICHARD L. , LUCHA WOLFGANG
Published: May 2007

It is shown that the ground-state eigenvalue of a semirelativistic Hamiltonian of the form [Formula: see text] is bounded below by the Schrödinger operator m + β p2 + V, for suitable β>0. An example is discussed.
WHY THERE IS SOMETHING SO CLOSE TO NOTHING: TOWARDS A FUNDAMENTAL THEORY OF THE COSMOLOGICAL CONSTANT
Authors: JEJJALA VISHNU , MINIC DJORDJE
Published: May 2007

The cosmological constant problem is turned around to argue for a new foundational physics postulate underlying a consistent quantum theory of gravity and matter, such as string theory. This postulate is a quantum equivalence principle which demands a consistent gauging of the geometric structure of canonical quantum theory. We argue that string theory can be formulated to accommodate such a principle, and that in such a theory the observed cosmological constant is a fluctuation about a zero value. This fluctuation arises from an uncertainty relation involving the cosmological constant and the effective volume of space–time. The measured, small vacuum energy is dynamically tied to the large "size" of the universe, thus violating naive decoupling between small and large scales. The numerical value is related to the scale of cosmological supersymmetry breaking, supersymmetry being needed for a nonperturbative stability of local Minkowski space–time regions in the classical regime.
DEVELOPMENT OF MULTIMODAL PRESENTATION MARKUP LANGUAGE MPML-HR FOR HUMANOID ROBOTS AND ITS PSYCHOLOGICAL EVALUATION
Authors: NISHIMURA YOSHITAKA , KUSHIDA KAZUTAKA , DOHI HIROSHI , ISHIZUKA MITSURU , TAKEUCHI JOHANE , NAKANO MIKIO , TSUJINO HIROSHI
Published: May 2007

Animated agents that act and speak as attendants to guests on shopping web sites are becoming increasingly popular. Inspired by this development, we propose a new method of presentation using a humanoid robot. Humanoid presentations are effective in a real environment because they can move and look around at the audience similar to a human presenter. We developed a simple script language for multimodal presentations by a humanoid robot called MPML-HR, which is a descendant of the Multimodal Presentation Markup Language (MPML) originally developed for animated agents. MPML-HR allows many non-specialists to easily write multimodal presentations for a humanoid robot. We further evaluated humanoid robots' presentation ability using MPML-HR to find the difference in audience impressions between the humanoid robot and the animated agent. Psychological evaluation was conducted to compare the impressions of a humanoid robot's presentation with an animated agent's presentation. Using the Semantic Differential (SD) method and direct questioning, we measured the difference in audience impressions between an animated agent and a humanoid robot.
CONTRIBUTION TO THE INTEGRATED CONTROL OF BIPED LOCOMOTION MECHANISMS
Authors: VUKOBRATOVIĆ MIOMIR K. , RODIĆ ALEKSANDAR D.
Published: May 2007

This work is concerned with the integrated dynamic control of humanoid locomotion mechanisms based on the spatial dynamic model of the humanoid mechanism, a servo system model, and an environment model. The control scheme was synthesized using the centralized model of the system and the hierarchical principle, with tactical and executive control levels. The proposed structure of the dynamic controller involves four feedback loops: position-velocity feedback at the robotic mechanism joints, dynamic reaction feedback at Zero-Moment Point, impact force feedback at the instant when the foot strikes the ground, and the load feedback of the mechanism joints. Simulation experiments are carried out for a number of characteristic examples. The numerical results obtained, along with theoretical study, serve as the basis for a critical evaluation of the performance of the devised controller.
DESIGN AND DEVELOPMENT OF FIVE-FINGERED HANDS FOR A HUMANOID EMOTION EXPRESSION ROBOT
Authors: ROCCELLA STEFANO , CARROZZA MARIA CHIARA , CAPPIELLO GIOVANNI , CABIBIHAN JOHN-JOHN , LASCHI CECILIA , DARIO PAOLO , TAKANOBU HIDEAKI , MATSUMOTO MUNEMICHI , MIWA HIROYASU , ITOH KAZUKO , TAKANISHI ATSUO
Published: May 2007

Among social infrastructure technologies, Robot Technology (RT) is expected to play an important role in solving the problems of the aging society. New generations of personal robots are expected to be capable of assisting humans in a variety of contexts and thus of interacting and communicating with them effectively and even in a friendly and natural way. Expressing human-like emotions is an important capability to this aim. The objectives of this work are the design and development of two five-fingered robotic hands for a humanoid upper body able to generate and express emotions. The specific design goals have been grasping and expression of emotions through hand gestures, as a complement to facial expression of emotions. The paper presents the design process of the robotic hands, named RCH-1 (Robocasa Hand No. 1), starting from the requirements deriving from their use in grasping and gestures. The resulting robotic hands are described in detail, together with the hand sensory systems. Experimental trials are then presented, aimed at assessing the hand performance and at validating their effectiveness in grasping and emotion expression, when mounted on the emotion expression humanoid robot WE-4R (Waseda Eye No. 4 Refined).
Paradoxical Role of Angiotensin II Type 2 Receptors in Resistance Arteries of Old Rats
Authors: Pinaud Frederic , Bocquet Arnaud , Dumont Odile , Retailleau Kevin , Baufreton Christophe , Andriantsitohaina Ramaroson , Loufrani Laurent , Henrion Daniel
Published: May 2007

The role of angiotensin II type 2 receptors (AT2Rs) remains a matter of controversy. Its vasodilatory and antitrophic properties are well accepted. Nevertheless, in hypertensive rats, AT2R stimulation induces a vasoconstriction counteracting flow-mediated dilation (FMD). This contraction is reversed by hydralazine. Because FMD is also decreased in aging, another risk factor for cardiovascular diseases, we hypothesized that AT2R function might be altered in old-rat resistance arteries. Mesenteric resistance arteries (250 μm in diameter) were isolated from old (24 months) and control (4 months) rats receiving hydralazine (16 mg/kg per day; 2 weeks) or water. FMD, NO-mediated dilation, and endothelial NO synthase expression were lower in old versus control rats. AT2R blockade improved FMD in old rats, suggesting that AT2R stimulation produced vasoconstriction. AT2R expression was higher in old rats and mainly located in the smooth muscle layer. In old rats, AT2R stimulation induced endothelium-independent contraction, which was suppressed by the antioxidant Tempol. Reactive oxygen species level was higher in old-rat arteries than in controls. Hydralazine improved FMD and NO-dependent dilation in old rats without change in AT2R expression and location. In old rats treated with hydralazine, reactive oxygen species level was reduced in endothelial and smooth muscle cells, and AT2R-dependent contraction was abolished. Thus, AT2R stimulation induced vasoconstriction through activation of reactive oxygen species production, contributing to decrease FMD in old-rat resistance arteries. Hydralazine suppressed AT2R-dependent reactive oxygen species production and AT2R-dependent contraction, improving FMD. Importantly, endothelial alterations in aging were reversible. These findings are important to consider in the choice of vasoactive drugs in aging.
Force-Induced Polarized Mitosis of Endothelial and Smooth Muscle Cells in Arterial Remodeling
Authors: Dajnowiec Dorota , Sabatini Peter J.B. , Van Rossum Thea C. , Lam Jacky T.K. , Zhang Ming , Kapus Andras , Langille B. Lowell
Published: May 2007

Arteries display highly directional growth and remodeling that are specific to increases in the mechanical loads imposed on them by blood pressure, blood flow, and lengthwise tensile forces that are transmitted from the tissues to which they are attached. This study examined the effect of mechanical forces on the direction in which mitosis delivers daughter cells, as a mechanism for directional growth. Lateral forces were imposed on surface integrins of cultured endothelial cells by seeding the cells with arginine-glycine-aspartate peptide–coated magnetic microspheres and applying a magnetic field. Video images revealed that the mitotic axis of dividing cells became highly biased in the direction of applied force. Distribution of cortactin, which participates in polarized mitoses driven by other stimuli, was highly sensitive to mechanical loading and interfering with cortactin function arrested cell growth. Smooth muscle cell mitoses also proved to be sensitive to mechanical force: when lengthwise force imposed on rabbit carotid arteries was altered by excision of a vessel segment and reanastomosis of the cut ends, direction of mitosis was dramatically altered. These findings indicate that influences of mechanical force can modulate the manner in which mitosis of vascular cells contributes to reorganization of arterial wall tissue.
Changes in Sleep Architecture following Motor Learning Depend on Initial Skill Level
Authors: Peters Kevin R. , Smith Valerie , Smith Carlyle T.
Published: May 2007

Previous research has linked both rapid eyemovement (REM) sleep and Stage 2 sleep to procedural memory consolidation. The present study sought to clarify the relationship between sleep stages and procedural memory consolidation by examining the effect of initial skill level in this relationship in young adults. In-home sleep recordings were performed on participants before and after learning the pursuit rotor task. We divided the participants into low- and high-skill groups based on their initial performance of the pursuit rotor task. In high-skill participants, there was a significant increase in Stage 2 spindle density after learning, and there was a significant correlation between the spindle density that occurred after learning and pursuit rotor performance at retest 1 week later. In contrast, there was a significant correlation between changes in REM density and performance on the pursuit rotor task during retest 1 week later in low-skill participants, although the actual increase in REM density failed to reach significance in this group. The results of the present study suggest the presence of a double dissociation in the sleep-related processes that are involved in procedural memory consolidation in low- and high-skill individuals. These results indicate that the changes in sleep microarchitecture that take place after learning depend on the initial skill level of the individual and therefore provide validation for the model proposed by Smith et al. [Smith, C. T., Aubrey, J. B., & Peters, K. R. Different roles for REM and Stage 2 sleep in motor learning. Psychologica Belgica, 44, 79–102, 2004]. Accordingly, skill level is an important variable that needs to be considered in future research on sleep and memory consolidation.
Mismatch Responses to Pitch Changes in Early Infancy
Authors: He Chao , Hotson Lisa , Trainor Laurel J.
Published: May 2007

We investigated the emergence of discriminative responses to pitch by recording 2-, 3-, and 4-month-old infants' electro-encephalogram responses to infrequent pitch changes in piano tones. In all age groups, infants' responses to deviant tones were significantly different from responses to standard tones. However, two types of mismatch responses were observed simultaneously in the difference waves. An increase in the left-lateralized positive slow wave was prominent in 2-month-olds, present in 3-month-olds, but insignificant in 4-month-olds. A faster adultlike mismatch negativity (MMN), lateralized to the right hemisphere, emerged at 2 months of age and became earlier and stronger as age increased. The coexistence and dissociation of two types of mismatch responses suggests different underlying neuromechanisms for the two responses. Furthermore, the earlier emergence of the MMN-like component to changes in pitch compared to other sound features implies that neural circuits involved in generating MMN-like responses have different maturational timetables for different sound features.
Modularity and the Social Mind
Authors: Kurzban Robert , Athena Aktipis C.
Published: May 2007

A modular view of the mind implies that there is no unitary “self” and that the mind consists of a set of informationally encapsulated systems, many of which have functions associated with navigating an inherently ambiguous and competitive social world. It is proposed that there are a set of cognitive mechanisms—a social cognitive interface (SCI)—designed for strategic manipulation of others' representations of one's traits, abilities, and prospects. Although constrained by plausibility, these mechanisms are not necessarily designed to maximize accuracy or to maintain consistency with other encapsulated representational systems. The modular view provides a useful framework for talking about multiple phenomena previously discussed under the rubric of the self.
Empirical, Theoretical, and Practical Advantages of the HEXACO Model of Personality Structure
Authors: Ashton Michael C. , Lee Kibeom
Published: May 2007

The authors argue that a new six-dimensional framework for personality structure—the HEXACO model— constitutes a viable alternative to the well-known Big Five or five-factor model. The new model is consistent with the cross-culturally replicated finding of a common six-dimensional structure containing the factors Honesty-Humility (H), Emotionality (E), eExtraversion (X), Agreeableness (A), Conscientiousness (C), and Openness to Experience (O). Also, the HEXACO model predicts several personality phenomena that are not explained within the B5/FFM, including the relations of personality factors with theoretical biologists' constructs of reciprocal and kin altruism and the patterns of sex differences in personality traits. In addition, the HEXACO model accommodates several personality variables that are poorly assimilated within the B5/FFM.
Themed Review: Anxiety/Depression
Authors: Martinsen Egil W. , Raglin John S.
Published: May 2007

Anxiety and depressive disorders are major public health problems, and desirable changes in lifestyle, such as exercise and proper nutrition, can be of great potential for prevention and treatment. There is growing evidence that physically active people are at a reduced risk of developing depression and that exercise interventions are associated with significant benefits for patients with mild to moderate forms of depression as well as in reducing anxiety. These findings have led to the proposal that exercise may serve as an alternative or a supplement to traditional forms of therapy. The prevalence of depression is lower in countries where the consumption of seafood is high, and omega-3 fatty acids seem to be beneficial in the management of mood disorders. Stimulants are often used as forms of self-medication in patients with anxiety or depressive disorders. Although providing short-term relief, they may have long-term adverse effects. This article presents a broad overview of research involving the efficacy of exercise as a means to treat depression and anxiety, as well as related issues regarding dosage and compliance. Research on the influence of nutrition and selected dietary supplements on depression and anxiety also are presented.
Behavioral Medecine Review: The Lifestyle Factor
Authors: Terre Lisa
Published: May 2007

Martinsen and Raglin provide a persuasive, evidence-based rationale for lifestyle approaches to the management of anxiety, depressive disorders, and their comorbidities. Yet, their review prompts discussion of the complexities navigating this terrain, including barriers to the identification and management of these common psychological problems in medical settings, the potential of these disorders to complicate health care, and challenges to the implementation of lifestyle interventions for depression and anxiety-related concerns.
Quantitative analysis of minimal residual disease predicts relapse in children with B-lineage acute lymphoblastic leukemia in DFCI ALL Consortium Protocol 95-01
Authors: Zhou Jianbiao , Goldwasser Meredith A , Li Aihong , Dahlberg Suzanne E. , Neuberg Donna , Wang Hongjun , Dalton Virginia , McBride Kathryn D , Sallan Stephen E. , Silverman Lewis B , Gribben John G.
Published: May 2007

In a prospective trial in 284 children with B-lineage acute lymphoblastic leukemia (ALL), we assessed the clinical utility of real-time quantitative polymerase chain reaction analysis of antigen receptor gene rearrangements for detection of minimal residual disease (MRD) to identify children at high risk of relapse. At the end of induction therapy, the 5-year risk of relapse was 5% in 176 children with no detectable MRD and 44% in 108 children with detectable MRD (P < .001), with a linear association of the level of MRD and subsequent relapse. Recursive partitioning and clinical characteristics identified that the optimal cutoff level of MRD to predict outcome was 10−3. The 5-year risk of relapse was 12% for children with MRD less than one leukemia cell per 103 normal cells (low MRD) but 72% for children with MRD levels greater than this level (high MRD) (P < .001) and children with high MRD had a 10.5-fold greater risk of relapse. Based upon these results we have altered our treatment regimen for children with B-lineage ALL and children with MRD levels greater than or equal to 10−3 at the end of 4 weeks of multiagent induction chemotherapy now receive intensified treatment to attempt to decrease their risk of subsequent relapse.
Alterations in cytoskeletal and immune function-related proteome profiles in whole rat lung following intratracheal instillation of heparin
Authors: Gabr Amir A , Reed Mathew , Newman Donna R , Pohl Jan , Khosla Jody , Sannes Philip L
Published: May 2007

Background

Heparin has been shown to modify fundamental biologic processes ranging from blood coagulation and cell proliferation to fibrogenesis and asthma. The goal of this study was to identify specific or broad biologic responses of the rat lung to intratracheal instillation of heparin by targeted proteomic analysis.

Methods

Rats were given either aerosolized 500 μg heparin in 250 μl saline or saline alone. Lungs were harvested at 0, 24, or 96 hours post-treatment and isolated proteins analyzed by two-dimensional gel electrophoresis. Proteins which increased and decreased significantly in treated groups above controls were then selected for identification by mass spectrometry.

Results

Although heparin treatments resulted in a general reduction in cytosolic protein expression, there were significant increases within members of discrete groups of proteins. At 24 hours, proteins which function in cytoskeletal organization and in calcium signaling were up-regulated between 2- and 27-fold above baseline and untreated controls. Increased proteins include annexins V and VI, septin 2, capping G protein, actin-related protein 3, moesin, RhoGDP dissociation inhibitor, and calcyclin. A group of proteins relating to immune response and tumor suppressor function were either up-regulated (tumor suppressor p30/hyaluronic acid binding protein-1, Parkinson disease protein 7, proteosome 28 subunit/interferon-γ inducible protein, and proteosome subunit macropain α-1) or strongly down-regulated (transgelin). At 96 hours, most proteins that had increased at 24 hours remained elevated but to a much lesser degree.

Conclusion

These cumulative observations demonstrate that whole lung heparin treatment results in significant up-regulation of selected groups of proteins, primarily those related to cytoskeletal reorganization and immune function, which may prove to be relevant biomarkers useful in analysis of lung exposures/treatments as well as in system biology studies.
Trial on Refinement of Early stage non-small cell lung cancer. Adjuvant chemotherapy with pemetrexed and cisplatin versus vinorelbine and cisplatin: The TREAT protocol
Authors: Kreuter Michael , Vansteenkiste Johan , Griesinger Frank , Hoffmann Hans , Dienemann Hendrik , De Leyn Paul , Thomas Michael
Published: May 2007

Background

Adjuvant chemotherapy has been proven to be beneficial for patients with early stage non-small cell lung cancer. However, toxicity and insufficient dose delivery have been critical issues with the chemotherapy used. Doublet regimens with pemetrexed, a multi-target folate inhibitor, and platin show clear activity in non-small cell lung cancer and are well tolerated with low toxicity rates and excellent delivery.

Methods/Design

In this prospective, multi-center, open label randomized phase II study, patients with pathologically confirmed non-small cell lung cancer, stage IB, IIA, IIB, T3N1 will be randomized after complete tumor resection either to 4 cycles of the standard adjuvant vinorelbine and cisplatin regimen from the published phase III data, or to 4 cycles of pemetrexed 500 mg/m2 d1 and cisplatin 75 mg/m2 d1, q 3 weeks. Primary objective is to compare the clinical feasibility of these cisplatin doublets defined as non-occurrence of grade 4 neutropenia and/or thrombocytopenia > 7 days or bleeding, grade 3/4 febrile neutropenia and/or infection, grade 3/4 non-hematological toxicity, non-acceptance leading to premature withdrawal and no cancer or therapy related death. Secondary parameters are efficacy (time to relapse, overall survival) and drug delivery. Parameters of safety are hematologic and non-hematologic toxicity of both arms.

Discussion

The TREAT trial was designed to evaluate the clinical feasibility, i.e. rate of patients without dose limiting toxicities or premature treatment withdrawal or death of the combination of cisplatin and pemetrexed as well as the published phase III regimen of cisplatin and vinorelbine. Hypothesis of the study is that reduced toxicities might improve the feasibility of drug delivery, compliance and the convenience of treatment for the patient and perhaps survival.

Trial Registration

Clinicaltrials.gov NCT00349089
Improving organisational systems for diabetes care in Australian Indigenous communities
Authors: Bailie Ross , Si Damin , Dowden Michelle , O'Donoghue Lynette , Connors Christine , Robinson Gary , Cunningham Joan , Weeramanthri Tarun
Published: May 2007

Background

Indigenous Australians experience disproportionately high prevalence of, and morbidity and mortality from diabetes. There is an urgent need to understand how Indigenous primary care systems are organised to deliver diabetes services to those most in need, to monitor the quality of diabetes care received by Indigenous people, and to improve systems for better diabetes care.

Methods

The intervention featured two annual cycles of assessment, feedback workshops, action planning, and implementation of system changes in 12 Indigenous community health centres. Assessment included a structured review of health service systems and audit of clinical records. Main process of care measures included adherence to guideline-scheduled services and medication adjustment. Main patient outcome measures were HbA1c, blood pressure and total cholesterol levels.

Results

There was good engagement of health centre staff, with significant improvements in system development over the study period. Adherence to guideline-scheduled processes improved, including increases in 6 monthly testing of HbA1c from 41% to 74% (Risk ratio 1.93, 95% CI 1.71–2.10), 3 monthly checking of blood pressure from 63% to 76% (1.27, 1.13–1.37), annual testing of total cholesterol from 56% to 74% (1.36, 1.20–1.49), biennial eye checking by a ophthalmologist from 34% to 54% (1.68, 1.39–1.95), and 3 monthly feet checking from 20% to 58% (3.01, 2.52–3.47). Medication adjustment rates following identification of elevated HbA1c and blood pressure were low, increasing from 10% to 24%, and from 13% to 21% respectively at year 1 audit. However, improvements in medication adjustment were not maintained at the year 2 follow-up. Mean HbA1c value improved from 9.3 to 8.9% (mean difference -0.4%, 95% CI -0.7;-0.1), but there was no improvement in blood pressure or cholesterol control.

Conclusion

This quality improvement (QI) intervention has proved to be highly acceptable in the Indigenous Australian primary care setting and has been associated with significant improvements in systems and processes of care and some intermediate outcomes. However, improvements appear to be limited by inadequate attention to abnormal clinical findings and medication management. Greater improvement in intermediate outcomes may be achieved by specifically addressing system barriers to therapy intensification through more effective engagement of medical staff in QI activities and/or greater use of nurse-practitioners.
Understanding the effects of a decentralized budget on physicians' compliance with guidelines for statin prescription – a multilevel methodological approach
Authors: Ohlsson Henrik , Merlo Juan
Published: May 2007

Background

Official guidelines that promote evidence-based and cost-effective prescribing are of main relevance for obvious reasons. However, to what extent these guidelines are followed and their conditioning factors at different levels of the health care system are still insufficiently known.

In January 2004, a decentralized drug budget was implemented in the county of Scania, Sweden. Focusing on lipid-lowering drugs (i.e., statins), we evaluated the effect of this intervention across a 25-month period. We expected that increased local economic responsibility would promote prescribing of recommended statins.

Methods

We performed two separate multilevel regression analyses; on 110 827 individual prescriptions issued at 136 publicly-administered health care centres (HCCs) nested within 14 administrative areas (HCAs), and on 72 012 individual prescriptions issued by 115 privately-administered HCCs. Temporal trends in the prevalence of prescription of recommended statins were investigated by random slope analysis. Differences (i.e., variance) between HCCs and between HCAs were expressed by median odds ratio (MOR).

Results

After the implementation of the decentralized drug budget, adherence to guidelines increased continuously. At the end of the observation period, however, practice variation remained high. Prescription of recommended statins presented a high degree of clustering within both publicly (i.e., MORHCC = 2.18 and MORHCA = 1.31 respectively) and privately administered facilities (MORHCC = 3.47).

Conclusion

A decentralized drug budget seems to promote adherence to guidelines for statin prescription. However, the high practice differences at the end of the observation period may reflect inefficient therapeutic traditions, and indicates that rational statin prescription could be further improved.
Effects of crp deletion in Salmonella enterica serotype Gallinarum
Authors: Rosu Valentina , Chadfield Mark S , Santona Antonella , Christensen Jens P , Thomsen Line E , Rubino Salvatore , Olsen John E
Published: May 2007

Background

Salmonella enterica serotype Gallinarum (S. Gallinarum) remains an important pathogen of poultry, especially in developing countries. There is a need to develop effective and safe vaccines. In the current study, the effect of crp deletion was investigated with respect to virulence and biochemical properties and the possible use of a deletion mutant as vaccine candidate was preliminarily tested.

Methods

Mutants were constructed in S. Gallinarum by P22 transduction from Salmonella Typhimurium (S. Typhimurium) with deletion of the crp gene. The effect was characterized by measuring biochemical properties and by testing of invasion in a chicken loop model and by challenge of six-day-old chickens. Further, birds were immunized with the deleted strain and challenged with the wild type isolate.

Results

The crp deletions caused complete attenuation of S. Gallinarum. This was shown by ileal loop experiments not to be due to significantly reduced invasion. Strains with such deletions may have vaccine potential, since oral inoculatoin with S. Gallinarum Δcrp completely protected against challenge with the same dose of wild type S. Gallinarum ten days post immunization. Interestingly, the mutations did not cause the same biochemical and growth changes to the two biotypes of S. Gallinarum. All biochemical effects but not virulence could be complemented by providing an intact crp- gene from S. Typhimurium on the plasmid pSD110.

Conclusion

Transduction of a Tn10 disrupted crp gene from S. Typhimurium caused attenuation in S. Gallinarum and mutated strains are possible candidates for live vaccines against fowl typhoid.
Threshold-free high-power methods for the ontological analysis of genome-wide gene-expression studies
Authors: Nilsson Björn , Håkansson Petra , Johansson Mikael , Nelander Sven , Fioretos Thoas
Published: May 2007

Ontological analysis facilitates the interpretation of microarray data. Here we describe new ontological analysis methods which, unlike existing approaches, are threshold-free and statistically powerful. We perform extensive evaluations and introduce a new concept, detection spectra, to characterize methods. We show that different ontological analysis methods exhibit distinct detection spectra, and that it is critical to account for this diversity. Our results argue strongly against the continued use of existing methods, and provide directions towards an enhanced approach.
Comprehensive Geriatric Assessment for Older Patients With Cancer
Authors: Extermann Martine , Hurria Arti
Published: May 2007

Purpose

During the last decade, oncologists and geriatricians have begun to work together to integrate the principles of geriatrics into oncology care. The increasing use of a comprehensive geriatric assessment (CGA) is one example of this effort. A CGA includes an evaluation of an older individual's functional status, comorbid medical conditions, cognition, nutritional status, psychological state, and social support; and a review of the patient's medications. This article discusses recent advances on the use of a CGA in older patients with cancer.

Methods

In this article, we provide an update on the studies that address the domains of a geriatric assessment applied to the oncology patient, review the results of the first studies evaluating the use of a CGA in developing interventions to improve the care of older adults with cancer, and discuss future research directions.

Results

The evidence from recent studies demonstrates that a CGA can predict morbidity and mortality in older patients with cancer. Accumulating data show the benefits of incorporating a CGA in the evaluation of older patients with cancer. Prospective trials evaluating the utility of a CGA to guide interventions to improve the quality of cancer care in older adults are justified.

Conclusion

Growing evidence demonstrates that the variables examined in a CGA can predict morbidity and mortality in older patients with cancer, and uncover problems relevant to cancer care that would otherwise go unrecognized.
Tribute to P. L. Lutz: cardiac performance and cardiovascular regulation during anoxia/hypoxia in freshwater turtles
Authors: Overgaard Johannes , Gesser Hans , Wang Tobias
Published: May 2007

Freshwater turtles overwintering in ice-covered ponds in North America may be exposed to prolonged anoxia, and survive this hostile environment by metabolic depression. Here, we review their cardiovascular function and regulation, with particular emphasis on the factors limiting cardiac performance. The pronounced anoxia tolerance of the turtle heart is based on the ability to match energy consumption with the low anaerobic ATP production during anoxia. Together with a well-developed temporal and spatial energy buffering by creatine kinase, this allows for cellular energy charge to remain high during anoxia. Furthermore, the turtle heart is well adapted to handle the adverse effects of free phosphate arising when phosphocreatine stores are used. Anoxia causes tenfold reductions in heart rate and blood flows that match the metabolic depression, and blood pressure is largely maintained through increased systemic vascular resistance. Depression of the heart rate is not driven by the autonomic nervous system and seems to arise from direct effects of oxygen lack and the associated hyperkalaemia and acidosis on the cardiac pacemaker. These intra- and extracellular changes also affect cardiac contractility, and both acidosis and hyperkalaemia severely depress cardiac contractility. However, increased levels of adrenaline and calcium may, at least partially, salvage cardiac function under prolonged periods of anoxia.
Characterization of circannual patterns of metabolic recovery from activity inRana catesbeianaat 15°C
Authors: Petersen A. M. , Gleeson T. T.
Published: May 2007

We characterized carbohydrate metabolism following activity in the American bullfrog, Rana catesbeiana, and compared whole body metabolic profiles between two seasons. Forty-eight adult male Rana catesbeianawere chronically cannulated and injected with[U-14C]l-lactic acid sodium salt in either summer (June)or winter (January) after acclimation for 2 weeks at 15°C with a 12 h:12 h L:D photoperiod. Following injection with [14C]lactate, frogs were either allowed to rest for 240 min (REST), hopped for 2 min on a treadmill and immediately sacrificed (PE), or hopped for 2 min on a treadmill and allowed to recover for 240 min (REC 4). Exercise caused a significant increase in blood lactate level from 2.7±0.1 mmol l–1 at rest to 17.0±2.1 mmol l–1 immediately following exercise. This increase persisted throughout the recovery period, with average blood lactate level only reduced to 13.7±1.1 mmol l–1 after 240 min of recovery, despite complete recovery of intramuscular lactate levels. Lactate levels were not significantly different between seasons in any treatment (REST, PE, REC4), in either gastrocnemius muscle or blood. The vast majority of [14C]lactate was recovered in the muscle, in both winter (86.3%) and summer (87.5%). Season had no effect on total amount of 14C label recovered. [14C]Lactate was measured in the forms of lactate, glucose and glycogen, in the liver and the muscle sampled. The most robust difference found in seasonal metabolism was that both the liver and the gastrocnemius contained significantly higher levels of intracellular free glucose under all treatments in winter. These data suggest that, overall, bullfrogs accumulate and slowly clear lactate in a manner quite similar to findings in fish, other amphibians and lizards. Additionally, our findings indicate that lactate metabolism is not highly influenced by season alone, but that intracellular glucose levels may be sensitive to annual patterns.
Tribute to P. L. Lutz: putting life on `pause' – molecular regulation of hypometabolism
Authors: Storey Kenneth B. , Storey Janet M.
Published: May 2007

Entry into a hypometabolic state is an important survival strategy for many organisms when challenged by environmental stress, including low oxygen, cold temperatures and lack of food or water. The molecular mechanisms that regulate transitions to and from hypometabolic states, and stabilize long-term viability during dormancy, are proving to be highly conserved across phylogenic lines. A number of these mechanisms were identified and explored using anoxia-tolerant turtles as the model system, particularly from the research contributions made by Dr Peter L. Lutz in his explorations of the mechanisms of neuronal suppression in anoxic brain. Here we review some recent advances in understanding the biochemical mechanisms of metabolic arrest with a focus on ideas such as the strategies used to reorganize metabolic priorities for ATP expenditure, molecular controls that suppress cell functions (e.g. ion pumping, transcription, translation, cell cycle arrest),changes in gene expression that support hypometabolism, and enhancement of defense mechanisms (e.g. antioxidants, chaperone proteins, protease inhibitors) that stabilize macromolecules and promote long-term viability in the hypometabolic state.
Tribute to P. L. Lutz: respiratory ecophysiology of coral-reef teleosts
Authors: Nilsson Göran E. , Hobbs Jean-Paul A. , Östlund-Nilsson Sara
Published: May 2007

One of the most diverse vertebrate communities is found on tropical coral reefs. Coral-reef fishes are not only remarkable in color and shape, but also in several aspects of physiological performance. Early in life, at the end of the pelagic larval stage, coral-reef fishes are the fastest swimmers of all fishes in relation to body size, and show the highest specific rates of maximum oxygen uptake. Upon settling on the reef, coral-reef fishes have to adopt a demersal lifestyle, which involves coping with a habitat that can become severely hypoxic, and some fishes may even have to rely on air breathing when their coral homes become air exposed. Oxygen availability appears to be a major ambient selection pressure, making respiratory function a key factor for survival on coral reefs. Consequently, hypoxia tolerance is widespread among coral-reef fishes. Hypoxia can even be a factor to gamble with for those fishes that are mouthbrooders, or a factor that the coral inhabitants may actively seek to reduce by sleep-swimming at night. Here, we summarize the present knowledge of the respiratory ecophysiology of coral-reef teleosts. From an ecophysiological perspective, the coral reef is an exciting and largely unexplored system for testing existing hypotheses and making new discoveries.