bluuebunny/crossref_metadata_2025 huggingface dataset:
“Augmenting the referral pathway for retinal services among diabetic patients at Reiyukai Eiko Masunaga Eye Hospital, Nepal: a non-randomized, pre-post intervention study”
Authors: Shrestha Ruchi , Singh Prerana , Dhakwa Parami , Tetali Shailaja , Batchu Tripura , Thapa Pragati Shrestha , Agiwal Varun , Pant Hira
Published: February 2023

Background

Diabetic Retinopathy (DR) is an important public health issue in Nepal. Despite the availability of retinal services, people may not access them because of the lack of knowledge about DR and poor referral systems. DR screening uptake was low at Reiyukai Eiko Masunaga Eye Hospital(REMEH) since retina services were started. Scheer Memorial Hospital is a multispeciality hospital near to REMEH. It has no eye department but has been running a regular diabetic clinic. This was a site for referring diabetic patients for DR screening. Improving DR awareness among general physicians has the potential to address these challenges.

Methods

The aim of our study was to investigate the effectiveness of providing health education to selected health personnel and establish a referral pathway on the attendance of diabetic patients for retinal screening at REMEH. This was a non-randomized, pre-post intervention study design. Total of three health education sessions were provided to the health care professionals of Scheer on diabetic retinopathy using Power Point presentations, posters, pamphlets and videos. The study period was 16 months (2020 June –2021 September) and divided into 8 months pre-intervention(baseline data collection) and 8 months post intervention period. The proportional increase in number of diabetes attendance pre and post intervention was calculated by Z test. The change in knowledge of health care personnels pre and post intervention was scored and evaluated through a questionnaire and calculated by paired- t test. Data was analyzed using Excel and Epi Info 7.The Protocol was published on August 21, 2021, in JMIR Publications.

Results

The proportional increase in number of referrals of diabetes attendance post intervention increased from 50 to 95% and was statistically significant (p < 0.001, 95% CI: 0.214–0.688). The mean score of knowledge gained by physicians on DR awareness was more at post intervention (8.8 ± 1.32) than pre intervention (6.4 ± 1.51). It was statistically significant (p < 0.001).

Conclusion

This study shows that a well-planned health education intervention changes the knowledge in physicians about DR. There is an increase in the number of referrals and attendance of patients for DR screening with the change in knowledge and referral mechanism.

Trial Registration

Clinical Trials.gov NCT04829084; https://clinicaltrials.gov/ct2/show/NCT04829084:02/04/2021.
Telemedicine in Swedish primary health care - a web-based survey exploring patient satisfaction
Authors: Rockler Meurling Carl , Adell Elisabet , Wolff Moa , Calling Susanna , Milos Nymberg Veronica , Borgström Bolmsjö Beata
Published: February 2023

Background

Direct-to-consumer telemedicine (TM), with patients having access to a physician via video or text chat, has gradually been introduced into Swedish primary care during the last two decades. Earlier studies have concluded that patients were generally satisfied with TM and the satisfaction rate was consistently above 80% and comparable with in-person visits. Despite the number of studies looking at user experience, studies assessing what factors influence patient satisfaction are lacking. To further develop digital care, it is important to explore the patients’ opinions of this relatively new phenomenon. The primary aim of this study was to explore patient opinions regarding satisfaction with TM-provided care, based on different age groups, sex, symptoms, and which type of health care professional they were assessed by.

Methods

The study was a quantitative web survey performed in 2020–2021 in which 688 patients using a TM provider in Southern Sweden responded. The results were analysed using Chi-2 test with the main outputs; satisfaction level and percentage that would use TM for similar symptoms in the future.

Results

The results from the survey population show that patients that were assessed by a doctor were more likely to intend to use TM in the future and were more satisfied with the visit than patients assessed by a nurse. Our results also show that patients older than 70 years of age were less likely to use TM again compared to the total population.

Conclusion

This study shows that patient satisfaction with TM varied depending on the patient’s age. The older patients were less satisfied than their younger equivalents. For patient satisfaction to be high, it was also crucial which health care provider the patient had been assessed by. The patients were more satisfied when assessed by a doctor than by a nurse. In addition, the study shows that patient satisfaction with TM was generally comparable to traditional care.
A modified renal risk score for Chinese patients with antineutrophil cytoplasmic antibody-associated vasculitis
Authors: Wang Rui-Xue , Wang Jin-Wei , Li Zhi-Ying , Chen Su-Fang , Yu Xiao-Juan , Wang Su-Xia , Zhang Fan , Xiong Zu-Ying , Bi Shu-Hong , Wang Yue , Zhao Ming-Hui , Chen Min
Published: February 2023

Background

The renal risk score (RRS) is a useful tool to predict end-stage renal disease (ESRD) in patients with antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). The current study aimed to validate the predictive performance of RRS and to further modify this model in Chinese AAV patients.

Methods

Two hundred and seventy-two patients diagnosed with AAV confirmed by renal biopsies were retrospectively enrolled from a single center. The RRS was calculated based on 3 categorical variables, i.e., the proportion of normal glomeruli, the proportion of interstitial fibrosis and tubular atrophy (IF/TA), and eGFR at biopsy, classifying these patients into low-, medium-, and high-risk groups. In addition, a modified model was developed based on the RRS and was further validated in another independent cohort of 117 AAV patients. The predictive performance of each model was evaluated according to discrimination and calibration.

Results

Patients were classified by the RRS into low- (26.5%), medium- (46.7%), and high-risk (26.8%) groups, with 120-month renal survival rates of 93.3%, 57.2%, and 18.4%, respectively (P < 0.001). The RRS showed good discrimination but less satisfactory calibration. Therefore, a modified model with improved discrimination and calibration was developed in Chinese AAV patients, with eGFR, proportion of normal glomeruli (both as continuous variables), and IF/TA (< 25%, 25–50%, > 50%) included. Internal and external validation of the modified model were performed. Finally, an online risk prediction tool was developed based on the modified model.

Conclusions

The RRS was an independent predictor of ESRD of AAV patients. The modified model could predict the probability of ESRD for AAV patients with improved performance in Chinese AAV patients.
Multiscale reconstruction of bronchus and cancer cells in human lung adenocarcinoma
Authors: Li Xin , Zhang Bowen , Liang Yanmei , Li Ting
Published: February 2023

Background

While previous studies primarily focused on the structure of the normal whole mouse lung, the whole bronchus and cytoarchitectural details of the mouse intact lung lobe have been discovered at single-cell resolution. Revealing the sophisticated lung adenocarcinoma structure at three-dimensional (3D) and single-cell level remains a fundamental and critical challenge for the pathological mechanism research of lung adenocarcinoma (LA).

Methods

Fluorescence micro-optical Sectioning Tomography (fMOST) combined with PI staining were used to obtain the 3D imaging of the human LA tissue at single-cell resolution.

Results

With a spatial resolution of 0.32 × 0.32 × 1.0 μm3, the dataset of human LA with single-cell precision consists of two channels, each of which contains information about the bronchi and the cytoarchitecture. The bronchial wall is thicker and the lumen is smaller in the cancer tissue, in which its original normal structure is vanished. More solid components, more clustered cancer cells with larger nucleoli, and more significant atypia are found in cancer tissue. In paracancerous tissue, the bronchial wall cells have a monolayer or bilayer structure, cluster along the wall, and are relatively dispersed. Few fibrous structures and occasional dissemination of spread through air spaces (STAS) are observed.

Conclusions

Based on the human LA tissue dataset obtained by fMOST and PI staining, the bronchi and cells were reconstructed and visualized. This work provides a technical roadmap for studying the bronchus and cytoarchitectural structure and their spatial relationship in LA tissue, which may help with the understanding of the main histological structure of LA among pathologists.
Transcriptome analysis of a newly established mouse model of Toxoplasma gondii pneumonia
Authors: Cheng Long , Rahman Sajid Ur , Gong Hai-Yan , Mi Rong-Sheng , Huang Yan , Zhang Yan , Qin Ju-Liang , Yin Cheng-Cong , Qian Min , Chen Zhao-Guo
Published: February 2023

Background

Toxoplasmosis is a zoonotic parasitic disease caused by Toxoplasma gondii. Toxoplasma gondii infection of the lungs can lead to severe pneumonia. However, few studies have reported Toxoplasma pneumonia. Most reports were clinical cases due to the lack of a good disease model. Therefore, the molecular mechanisms, development, and pathological damage of Toxoplasma pneumonia remain unclear.

Methods

A mouse model of Toxoplasma pneumonia was established by nasal infection with T. gondii. The model was evaluated using survival statistics, lung morphological observation, and lung pathology examination by hematoxylin and eosin (H&E) and Evans blue staining at 5 days post-infection (dpi). Total RNA was extracted from the lung tissues of C57BL/6 mice infected with T. gondii RH and TGME49 strains at 5 dpi. Total RNA was subjected to transcriptome analysis by RNA sequencing (RNA-seq) followed by quantitative real-time polymerase chain reaction (qRT–PCR) validation. Transcript enrichment analysis was performed using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases to assess the biological relevance of differentially expressed transcripts (DETs).

Results

C57BL/6 mice infected with T. gondii via nasal delivery exhibited weight loss, ruffled fur, and respiratory crackles at 5 dpi. The clinical manifestations and lethality of RH strains were more evident than those of TGME49. H&E staining of lung tissue sections from mice infected with T. gondii at 5 dpi showed severe lymphocytic infiltration, pulmonary edema, and typical symptoms of pneumonia. We identified 3167 DETs and 1880 DETs in mice infected with the T. gondii RH and TGME49 strains, respectively, compared with the phosphate-buffered saline (PBS) control group at 5 dpi. GO and KEGG enrichment analyses of DETs showed that they were associated with the immune system and microbial infections. The innate immune, inflammatory signaling, cytokine-mediated signaling, and chemokine signaling pathways displayed high gene enrichment.

Conclusion

In this study, we developed a new mouse model for Toxoplasma pneumonia. Transcriptome analysis helped to better understand the molecular mechanisms of the disease. These results provided DETs during acute T. gondii lung infection, which expanded our knowledge of host immune defenses and the pathogenesis of Toxoplasma pneumonia.

Graphical Abstract
Efficacy of fluralaner chewable tablets (Bravecto®) against Asian longhorned tick (Haemaphysalis longicornis) infestations of dogs
Authors: Petersen Melissa , Maree Riaan , Viljoen Alta , Liebenberg Julian E. , Guerino Frank
Published: February 2023

Background

The parthenogenic reproductive ability of Haemaphysalislongicornis, facilitating quick life cycle completion and rapid geographic spread and its pathogen vector potential make infestations a risk to human and canine health. Two 90-day studies were initiated to evaluate the efficacy of a single fluralaner administration for the treatment and prevention of H.longicornis infestations on dogs.

Methods

Dogs were randomly assigned (10 dogs/group) to either an untreated control group or a group treated once (Day 0) with 13.64% w/w fluralaner chewable tablets (Bravecto®) at the minimum label dose rate of 25 mg/kg. Each dog was infested with approximately 50 H.longicornis ticks on Days -9 or -6 and on Days -2, 28, 58 and 88. A different US tick isolate was used in each study. Tick counts were completed on Days -7 or -4, 2, 30, 60 and 90. The primary efficacy criterion was a 90% reduction in arithmetic mean tick counts between the treated and control groups. For between-group comparisons at any assessment, at least six control dogs were required to retain at least 25% of the infestation dose (13 live ticks).

Results

Pre-study infestations demonstrated susceptibility of all study dogs to challenge with H.longicornis. At each subsequent assessment in both studies, at least seven untreated control dogs retained ≥ 25% of the challenge, demonstrating adequate infestations for each efficacy calculation. On Days 2, 30, 60 and 90 the mean live tick infestation rate (number of ticks recovered from each dog/infesting challenge of each dog) of untreated control dogs ranged from 27.8 to 60.8%. No live ticks, free or attached, were found on any fluralaner-treated dog in either study. Between-group differences were statistically significant (P ≤ 0.0002) at each assessment.

Conclusion

At the minimum recommended label dose rate of 25 mg/kg, fluralaner chewable tablets were 100% effective in eliminating H.longicornis ticks from dogs infested at the time of treatment. Complete efficacy against both US isolates of this tick was maintained through 90 days following a single treatment. Therefore, fluralaner is a treatment of choice for protecting dogs against this invasive tick species.

Graphical abstract
The effects of exercise therapy feedback on subjective treatment outcome and patient satisfaction: study protocol for a mono-centric, randomized, controlled trial in orthopedic rehabilitation (FeedYou)
Authors: Schuber André Arik , Schmidt Sebastian , Hombach Sarah , Schaller Andrea
Published: February 2023

Background

The disease burden of musculoskeletal disorders necessitates multidisciplinary and patient-centered models of care. Exercise therapy represents a first-line treatment strategy and a central component of medical rehabilitation. In order to realize the goals of long-term physical activity and participation as proposed by the ICF, exercise therapy can be supplemented by interventional techniques from the field of psychotherapy. Although psychotherapist feedback has been shown to improve therapeutic outcome and patient satisfaction, feedback use in exercise therapy is mostly limited to motor learning and exercise instruction. The present paper therefore describes the use of multidimensional exercise therapy feedback in medical rehabilitation. The aims of the trial presented in this study protocol are to evaluate the effects of this novel feedback approach on rehabilitation outcomes in comparison to usual care.

Methods

The study is designed as a prospective, mono-centric, randomized controlled, superiority trial (RCT) with two parallel groups and three measuring points: T0 = start of three-week inpatient rehabilitation, T1 = end of three-week inpatient rehabilitation, T2 = 12-week follow-up. In total, 132 patients suffering from chronic neck, shoulder and/or lumbar spine disorders will be recruited. The intervention involves multidimensional exercise therapy feedbacks during the initial and final physical therapist examination, as well as short exercise therapy feedbacks during the course units of the mandatory group-based exercise therapy program. Primary outcomes are the subjective treatment outcome, assessed by BPI and indication-specific questionnaires, as well as patient satisfaction, assessed by ZUF-8 and an intervention-specific questionnaire. The final data collection is expected by May 2023.

Discussion

This study may provide a valuable insight into the effectiveness of multidimensional exercise therapy feedback to improve treatment outcomes and patient satisfaction in medical rehabilitation. This could contribute to rehabilitation quality assurance and the long-term physical activity behavior of rehabilitation patients.

Trial registration The trial has been registered with the German Clinical Trial Register (DRKS) under the Registration Number DRKS00027263.
Concurrence of immune thrombocytopenic purpura and thrombotic thrombocytopenic purpura: a case report and review of the literature
Authors: Lin Hung-Chen , Huang Jinxiang , Huang Jing , Zhang Li-Jun , Yin Xiao-Wu , Yang Jian-Cheng , Huang Xiao-Yan
Published: February 2023

Background

Immune thrombocytopenic purpura and thrombotic thrombocytopenic purpura are both causes of thrombocytopenia. Recognizing thrombotic thrombocytopenic purpura is crucial for subsequent treatment and prognosis. In clinical practice, corticosteroids and rituximab can be used to treat both immune thrombocytopenic purpura and thrombotic thrombocytopenic purpura; plasma exchange therapy is the first-line treatment in thrombotic thrombocytopenic purpura, while corticosteroids are strongly recommended as first-line treatment in immune thrombocytopenic purpura. The differential diagnosis of immune thrombocytopenic purpura and thrombotic thrombocytopenic purpura is essential in clinical practice. However, case reports have suggested that immune thrombocytopenic purpura and thrombotic thrombocytopenic purpura can occur concurrently.

Case presentation

We report the case of a 32-year-old Asian female without previous disease who presented with pancytopenia, concurrent with immune thrombocytopenic purpura and thrombotic thrombocytopenic purpura. The morphology of the megakaryocytes in the bone marrow indicated immune-mediated thrombocytopenia. The patient received glucocorticoid treatment, and her platelet count increased; however, schistocytes remained high during the course of the therapy. Further investigations revealed ADAMTS13 activity deficiency and positive ADAMTS13 antibodies. The high titer of antinuclear antibody and positive anti-U1-ribonucleoprotein/Smith antibody indicated a potential autoimmune disease. However, the patient did not fulfill the current criteria for systemic lupus erythematosus or mixed connective tissue disease. The patient responded well to plasma exchange therapy, and her platelet count remained normal on further follow-up.

Conclusions

Concurrence of immune thrombocytopenic purpura and thrombotic thrombocytopenic purpura is rare, but clinicians should be aware of this entity to ensure prompt medical intervention. Most of the reported cases involve young women. Human immunodeficiency virus infection, pregnancy, and autoimmune disease are the most common underlying conditions.
Existence and stability results for nonlinear fractional integrodifferential coupled systems
Authors: Zhou Jue-liang , He Yu-bo , Zhang Shu-qin , Deng Hai-yun , Lin Xiao-yan
Published: February 2023

In this paper, a class of nonlinear ψ-Hilfer fractional integrodifferential coupled systems on a bounded domain is investigated. The existence and uniqueness results for the coupled systems are proved based on the contraction mapping principle. Moreover, the Ulam–Hyers–Rassias, Ulam–Hyers, and semi-Ulam–Hyers–Rassias stabilities to the initial value problem are obtained.
Tamoxifen induces radioresistance through NRF2-mediated metabolic reprogramming in breast cancer
Authors: Reinema F. V. , Sweep F. C. G. J. , Adema G. J. , Peeters W. J. M. , Martens J. W. M. , Bussink J. , Span P. N.
Published: February 2023

Background

Recently, we reported that tamoxifen-resistant (TAM-R) breast cancer cells are cross-resistant to irradiation. Here, we investigated the mechanisms associated with tamoxifen-induced radioresistance, aiming to prevent or reverse resistance and improve breast cancer treatment.

Methods

Wild-type ERα-positive MCF7 and ERα-negative MDA-MB-231 breast cancer cells and their TAM-R counterparts were analyzed for cellular metabolism using the Seahorse metabolic analyzer. Real-time ROS production, toxicity, and antioxidant capacity in response to H2O2, tamoxifen, and irradiation were determined. Tumor material from 28 breast cancer patients before and after short-term presurgical tamoxifen (ClinicalTrials.gov Identifier: NCT00738777, August 19, 2008) and cellular material was analyzed for NRF2 gene expression and immunohistochemistry. Re-sensitization of TAM-R cells to irradiation was established using pharmacological inhibition.

Results

TAM-R cells exhibited decreased oxygen consumption and increased glycolysis, suggesting mitochondrial dysfunction. However, this did not explain radioresistance, as cells without mitochondria (Rho-0) were actually more radiosensitive. Real-time measurement of ROS after tamoxifen and H2O2 exposure indicated lower ROS levels and toxicity in TAM-R cells. Consistently, higher antioxidant levels were found in TAM-R cells, providing protection from irradiation-induced ROS. NRF2, a main activator of the antioxidant response, was increased in TAM-R cells and in tumor tissue of patients treated with short-term presurgical tamoxifen. NRF2 inhibition re-sensitized TAM-R cells to irradiation.

Conclusion

Mechanisms underlying tamoxifen-induced radioresistance are linked to cellular adaptations to persistently increased ROS levels, leading to cells with chronically upregulated antioxidant capacity and glycolysis. Pharmacological inhibition of antioxidant responses re-sensitizes breast cancer cells to irradiation.
Publication rates of research presented at the Canadian Society of Otolaryngology-Head and Neck Surgery Annual Meetings from 2008 to 2018: An 11-year review
Authors: Feschuk Aileen M. , Hathi Kalpesh , Taylor S. Mark
Published: February 2023

Background

Knowledge dissemination is paramount so physicians may practice the most up-to-date, evidence-based medicine to best serve their patients. Medical conferences are a commonly employed method of facilitating this. By determining the publication rate of research presented at a conference, the quality of the conference is indirectly assessed. Therefore, this study aimed to determine the publication rate, along with other conference metrics, of abstracts presented at the Canadian Society of Otolaryngology-Head and Neck Surgery (CSOHNS) meetings from 2008 to 2018.

Methods

All abstracts presented at the CSOHNS Annual Meetings from 2008 to 2018 were reviewed from publicly available records. Presentation year, presentation type (i.e. oral or poster), whether each abstract was presented in the Poliquin Resident Research Competition, and the country in which the lead author's institution was located, were collected. Publication status of each abstract was then determined using a six-tiered search strategy in PubMed and Google Scholar. All data were then analyzed using SPSS Version 27.0.

Results

From 2008 to 2018, 1947 abstracts were analyzed, yielding an overall publication rate of 58.7%. There was a significantly increasing trend in publication rate over the 11 years (p = 0.015). The rate of publication differed based on type of presentation (oral 65.1%, poster 50.2%; p = 0.001). Most presentations were presented by a first author associated with a Canadian institution (94.4%). The top journal in which research was published was Journal of Otolaryngology- Head and Neck Surgery (37.3%). The mean impact factor of the journals in which presentations were published was 2.92. Finally, the median time to publication was 14 months (IQR: 9.0–25.0).

Conclusions

Research presented at 2008–2018 CSOHNS annual meetings was published in academic journals at higher rates than research at comparable conferences. Oral presentations have a significantly greater publication rate, compared to poster presentations. Additionally, the upward trend in publication rate over the 11 meetings suggests a steady increase in the quality of research being presented.

Graphical Abstract
Accuracy of Doppler ultrasound in the diagnosis of giant cell arteritis: a systematic review and meta-analysis
Authors: Nakajima Eliza , Moon Francisca Hatta , Junior Nelson Carvas , Macedo Cristiane Rufino , de Souza Alexandre Wagner Silva , Iared Wagner
Published: February 2023

Background

Giant cell arteritis (GCA) is the most common primary systemic vasculitis in people 50 years of age and over, and it is considered a medical emergency due to the potential risk of permanent visual loss. Color Doppler ultrasound (CDU) of the temporal arteries is a rapid, noninvasive method to diagnose GCA. This study aims to determine the diagnostic accuracy of the halo sign in temporal arteries by CDU in people with suspected GCA.

Methods

The systematic literature review included the search for publications in the following electronic databases: PubMed, Embase, CENTRAL, LILACS, WHO ICTRP, ClinicalTrials.gov, gray literature up to December 2022, and no date or language restrictions were applied. We analyzed studies including patients over 50 years of age with suspected GCA evaluating CDU of temporal arteries as a diagnostic tool against clinical diagnosis as a standard reference. Paper titles and abstracts were selected by two investigators independently for all available records. The quality of the studies was assessed using the Quality of Diagnostic Accuracy Studies tool (QUADAS-2) and the R software (version 4.2.1) was used for data analysis. The protocol of this review is registered with PROSPERO (CRD42016033079).

Results

Twenty-two studies including 2893 participants with suspected GCA who underwent temporal artery CDU were evaluated. The primary analysis results showed a sensitivity of 0.76 [95% confidence interval (95 CI) 0.69–0.81] and specificity of 0.93 (95 CI 0.89–0.95) when the halo sign was compared to clinical diagnosis. The sensitivity value of 0.84 (95 CI 0.72–0.92) and specificity of 0.95 (95 CI 0.88–0.98) were found in five studies involving 1037 participants that analyzed the halo sign and temporal artery compression sign. A sensitivity of 0.86 (95 CI 0.78–0.91) and specificity of 0.95 (95 CI 0.89–0.98) were found in four studies with 603 participants where the halo sign was evaluated CDU on temporal and axillary arteries.

Conclusion

The detection of the halo sign by CDU of temporal arteries has good accuracy for the diagnosis of cranial GCA. The compression sign in temporal arteries and the addition of axillary arteries assessment improves the diagnostic performance of CDU for GCA.

Trial registration

PROSPERO CRD42016046860.
NtMYB12 requires for competition between flavonol and (pro)anthocyanin biosynthesis in Narcissus tazetta tepals
Authors: Yang Jingwen , Wu Xi , Aucapiña Cristina belen , Zhang Deyu , Huang Jiazhi , Hao Ziyuan , Zhang Yu , Ren Yujun , Miao Ying
Published: February 2023

The color of flowers is one of the main characteristics adopted for plants to attract pollinators to ensure the reproductive success of the plant, they are also important in their ornamental appeal in Narcissus plant. In this study, we identified a NtMYB12 locus encoding an R2R3-MYB transcription factor. Comparative transcriptome analysis of loss- and gain- of NtMYB12 tissue relative to wild-type narcissus showed NtMYB12 was mainly involved in flavonol and phenylpropanoid metabolic pathways. Biochemical evidences of dual-luciferase activity and chromatin immunoprecipitation assay supported that MYB12 directly bound to promoters of NtFLS, NtLAR, and NtDFR that were cloned by genome walking assay, and activated NtFLS and NtLAR expression but repressed NtDFR expression. More interestingly, NtMYB12 can interact with NtbHLH1 and NtWD40-1 proteins via R3 domain that were selected by transcriptome-based WGCNA and confirmed by yeast two hybrid, bimolecular fluorescence complementation and coimmunoprecipitation assay. Interaction of NtMYB12 with NtbHLH1 and NtWD40-1 forming MYB-bHLH-WD40 triplex specially activated NtDFR and NtANS expression and promoted (pro)anthocyanin accumulation, while NtMYB12 alone activated NtFLS and NtLAR expression and accumulated flavonols, but repressed NtDFR expression. These results indicated that NtMYB12 alone or NtMYB12-bHLH1-WD40-1 triplex requires for competition of metabolism fluxes between flavonol and (pro)anthocyanin biosynthesis.

Graphical Abstract

NtMYB12 dually functions on
flavonol and proanthocyanin biogenesis via
physically binding to NtFLS and NtLAR promoter activating their expression and on (pro)anthocyanin biosynthesis via NtMYB12-NtWD40-NtbHLH (MBW) triplex activating NtDFR and NtANS expression. Requirement of NtMYB12 alone or MBW complex for the competition between flavonol and anthocyanin biosynthesis results in narcissus colorized petal traits.
Maximum correntropy self-organizing map for waveform classification
Authors: Shen Shi’an , Wei Xinjian , Wang Xiaokai , Chen Wenchao
Published: February 2023

The self-organizing map (SOM) is a well-known unsupervised classification method for seismic facies analysis. The conventional SOM networks and their mutation methods are mostly based on the Euclidean distance measurement, which estimates the global similarity between the input data and weights of neuron. Such measurement is suitable for signals with a Gaussian distribution but may not work well for seismic data for which the dominant structural features (e.g., stratigraphic layers, faults, channels, and salt bodies) typically appear some specially distributed patterns. Therefore, we propose to modify the SOM by introducing a new distance measurement criterion based on the maximum correntropy. The correntropy is mainly used to measure the local similarity between variables, especially for non-Gaussian signals, and therefore is more suitable than the Euclidean distance to adaptively characterize the seismic structural similarities for seismic classification tasks. Validations on two synthetic data sets indicate that the proposed method yields more accurate classification results and is more robust to noise than the traditional SOM algorithm. Comparison experiments on a field data example also demonstrate that the proposed method can delineate the distribution of seismic facies more effectively and stably than the traditional one.
The determinants of the individual nestedness contribution in financial systems
Authors: Alexandre M. , Xavier F. , Silva T. , Rodrigues F.
Published: February 2023

Nestedness is one of the most pervasive and studied patterns observed in complex networks and refers to a hierarchical organization of the network. In this paper, we assess the determinants of the individual nestedness contribution (INC) in financial systems. To perform this task, we rely on data from two Brazilian financial networks: the bank-firm credit network and the interbank network. We computed the nestedness of the networks, as well as the INC for each node. The main determinants of the INC in the bank-firm network are the degree and the core number. In the interbank network, the INC of lending banks is mainly driven by their degree, while the INC of borrowing banks has not a clear main determinant.
Thermal performance curve of endurance running at high temperatures in deer mice
Authors: Eizenga Matthew R. , Flewwelling Luke D. , Warrier Tanisha , Scott Graham R.
Published: February 2023

The impacts of warming temperatures associated with climate change on performance are poorly understood in most mammals. Thermal performance curves are a valuable means of examining the effects of temperature on performance traits, but they have rarely been used in endotherms. Here, we examined the thermal performance curve of endurance running capacity at high temperatures in the deer mouse (Peromyscus maniculatus). Endurance capacity was measured using an incremental speed test on a treadmill, and subcutaneous temperature in the abdominal region was measured as a proxy for body temperature (Tb). Endurance time at 20°C was repeatable but varied appreciably across individuals, and was unaffected by sex or body mass. Endurance capacity was maintained across a broad range of ambient temperatures (Ta) but was reduced above 35°C. Tb during running varied with Ta, and reductions in endurance were associated with Tb greater than 40°C when Ta was above 35°C. At the high Ta that limited endurance running capacity (but not at lower Ta), Tb tended to rise throughout running trials with increases in running speed. Metabolic and thermoregulatory measurements at rest showed that Tb, evaporative water loss and breathing frequency increased at Ta of 36°C and above. Therefore, the upper threshold temperatures at which endurance capacity is impaired are similar to those inducing heat responses at rest in this species. These findings help discern the mechanisms by which deer mice are impacted by warming temperatures, and provide a general approach for examining thermal breadth of performance in small mammals.
Accuracy and precision of contrast enhanced mammography versus MRI for predicting breast cancer size: how “good” are they really?
Authors: Taylor Donna Blanche , Burrows Sally , Dessauvagie Benjamin Frederik , Saunders Christobel Mary , Ives Angela
Published: February 2023

Objective:

To evaluate and compare the accuracy and precision of contrast-enhanced mammography (CEM) vs MRI to predict the size of biopsy-proven invasive breast cancer.

Methods:

Prospective study, 59 women with invasive breast cancer on needle biopsy underwent CEM and breast MRI. Two breast radiologists read each patient’s study, with access limited to one modality. CEM lesion size was measured using low-energy and recombined images and on MRI, the first post-contrast series. Extent of abnormality per quadrant was measured for multifocal lesions. Reference standards were size of largest invasive malignant lesion, invasive (PathInvasive) and whole (PathTotal). Pre-defined clinical concordance ±10 mm.

Results:

Mean patient age 56 years, 42 (71%) asymptomatic. Lesions were invasive ductal carcinoma 40 (68%) with ductal carcinoma in situ (31/40) in 78%, multifocal in 12 (20%). Median lesion size was 17 mm (invasive) and 27 mm (total), range (5–125 mm). Lin’s concordance correlation coefficients for PathTotal 0.75 (95% CI 0.6, 0.84) and 0.71 (95% CI 0.56, 0.82) for MRI and contrast-enhanced spectral mammography (CESM) respectively. Mean difference for total size, 3% underestimated and 4% overestimated, and for invasive 41% and 50% overestimate on MRI and CESM respectively. LOAs for PathTotal varied from 60% under to a 2.4 or almost threefold over estimation. MRI was concordant with PathTotal in 36 (64%) cases compared with 32 (57%) for CESM. Both modalities concordant in 26 (46%) cases respectively.

Conclusion

Neither CEM nor MRI have sufficient accuracy to direct changes in planned treatment without needle biopsy confirmation.

Advances in knowledge:

Despite small mean differences in lesion size estimates using CEM or MRI, the 95% limits of agreement do not meet clinically acceptable levels.
What and how do different stakeholders contribute to intervention development? A mixed methods study.
Authors: Racine Emmy , O Mahony Lauren , Riordan Fiona , Flynn Gráinne , Kearney Patricia M. , McHugh Sheena M.
Published: February 2023

Background: UK Medical Research Council guidelines recommend end-user involvement in intervention development. There is limited evidence on the contributions of different end-users to this process. The aim of this Study Within A Trial (SWAT) was to identify and compare contributions from two groups of end-users - people with diabetes’ (PWD) and healthcare professionals’ (HCPs), during consensus meetings to inform an intervention to improve retinopathy screening uptake.

Methods: A mixed method, explanatory sequential design comprising a survey and three semi-structured consensus meetings was used. PWD were randomly assigned to a PWD only or combined meeting. HCPs attended a HCP only or combined meeting, based on availability. In the survey, participants rated intervention proposals on acceptability and feasibility. Survey results informed the meeting topic guide. Transcripts were analysed deductively to compare feedback on intervention proposals, suggestions for new content, and contributions to the final intervention.

Results: Overall, 13 PWD and 17 HCPs completed the survey, and 16 PWD and 15 HCPs attended meetings. For 31 of the 39 intervention proposals in the survey, there were differences (≥10%) between the proportion of HCPs and PWD who rated proposals as acceptable and/or feasible. End-user groups shared and unique concerns about proposals; both were concerned about informing but not scaring people when communicating risk, while concerns about resources were mostly unique to HCPs and concerns about privacy were mostly unique to PWD.  Fewer suggestions for new intervention content from the combined meeting were integrated into the final intervention as they were not feasible for implementation in general practice. Participants contributed four new behaviour change techniques not present in the original proposals: goal setting (outcome), restructuring the physical environment, material incentive (behaviour) and punishment.

Conclusions: Preferences for intervention content may differ across end-user groups, with feedback varying depending on whether end-users are involved simultaneously or separately.
Digital tutor. The use of e-learning and distance learning technologies in pedagogical practice
Authors: Tkachenko T.Ya. , Neupokoeva L.Yu. , Shestakova A.A. , Koroleva M.E. , Berhold N.B.
Published: February 2023

"Цель ДПП ПК: удовлетворение образовательных и профессиональных потребностей, профессионального развития человека, обеспечении соответствия его квалификации меняющимся условиям профессиональной деятельности и социальной среды; углубление знаний в области использования электронного обучения (ЭО) и дистанционных образовательных технологий (ДОТ) в педагогической практике. Задачи ДПП ПК: Овладение теоретическими основами и практическими навыками современных подходов к использованию электронного обучения (ЭО) и дистанционных образовательных технологий (ДОТ) в педагогической практике. Изучение нормативно-правовой базы, регулирующей применение дистанционных технологий в системе образования. Формирование и совершенствование умений использования инструментария цифровой образовательной среды медицинского университета в условиях трансформации дистанционного обучения. Категория обучающихся: Лица, имеющие среднее специальное или высшее образование по медицинским и/или педагогическим специальностям, а также сотрудники кафедр, аспиранты или ординаторы, имеющие навыки работы с современными информационными технологиями, участвующие в реализации программ медицинского образования всех уровней с применением ЭО и ДОТ."
Chat-bot "DigitalChiefBot"
Authors: Tkachenko T.Ya. , Neupokoeva L.Yu. , Shestakova A.A. , Shestakov I.V.
Published: February 2023

Цифровой бот помогает найти ответы на распространённые вопросы, возникающие у преподавателей, которые применяют в своей деятельности цифровые образовательные технологии. «Цифровой заведующий кафедрой» (DigitalChiefBot)- предоставляет информацию по всем направлениям электронного обучения и дистанционных образовательных технологий, реализуемых в УГМУ, а именно: работа в системе дистанционного обучения MedSpace; создание, заполнение и экспертиза электронных учебных курсов; полезная информация по развитию цифровых компетенций сотрудников; организация и проведение дистанционных лекций и семинарских занятий; взаимодействие с мультимедиацентром УГМУ для записи видеороликов, видеолекций, лабораторных работ и др.
Electronic training course "Fundamentals of medical law"
Authors: Bogdanova E.N.
Published: February 2023

Курс основы медицинского права сочетает в себе нормы различных отраслей права и правовых институтов. Особенности медицинской деятельности, специфика ее правового регулирования обусловливают междисциплинарный характер данного курса. Цель изучения курса заключается в приобретении студентами-медиками теоретических знаний в области медицинского законодательства, в привитии навыков анализа источников и использования принципов и норм права данной отрасли в практической деятельности врача. Задачами курса являются: ознакомление с особенностями медицинских правоотношений; ознакомление с правовым значением врачебной этики, деонтологии и биоэтики; получение студентами базовых знаний по организации здравоохранения и медицинского страхования; изучение порядка и условий оказания платных медицинских услуг; ознакомление с правовым регулированием оказания отдельных видов медицинской помощи; изучение правовой квалификации дефектов оказания медицинской помощи; приобретение навыков применения действующего права к ситуациям, возникающим на практике врача. Использование электронного учебного курса предполагает формирование коммуникативной и учебно-познавательной компетенций студентов, умений извлекать информацию из различных правовых источников, отрабатывать самостоятельные навыки работы в условиях удаленного доступа. Электронный учебный курс состоит из тематических блоков, каждый из которых содержит следующие разделы: теоретический материал, практикум. Теоретический раздел состоит из тематического плана, информационных источников по каждой теме раздела. Раздел «практикум» содержит тестовые задания, ситуационные задачи, задания для самостоятельной работы, темы рефератов. Доступность и простота интерфейса не требует специальной подготовки. Электронный учебный курс открывает новые возможности для модернизации преподавания курса.
Electronic educational resource "Consumer rights Protection"
Authors: Kozlovskih Dmitrij , Kosova Anna , Dikonskaya Olga , Romanov Sergey , Shuleshova Tatyana , Cunina Yuliya , Galnykina Irina , Trofimova Svetlana , Egorova Viktoriya , Suhanov Igor , Sheluncova Nataliya , Zhuravleva Valeriya , Korolenko Irina , Nabeeva Olga , Minikeeva Anastasiya , Myachina Tatyana
Published: February 2023

Электронный образовательный ресурс «Защита прав потребителей» составлен в соответствии с требованиями Федерального государственного образовательного стандарта высшего образования специальность 32.05.01 Медико-профилактическое дело, утвержденным приказом Министерства образования и науки Российской Федерации от 16 января 2017 г. №21 (с изменениями от 13.07.2017 г.), и с учетом требований профессионального стандарта 02.002 «Специалист в области медико-профилактического дела», утвержденного приказом Министерства труда и социальной защиты Российской Федерации от 25 июня 2015 г. №399н. Цель изучения курса - сформировать знания, умения и навыки, необходимые специалисту для работы в области защиты прав потребителей, дать будущим специалистам Роспотребнадзора необходимый и оптимальный объем правовых знаний по вопросам защиты прав потребителей, позволяющий аргументировано принимать решения при организации и осуществлении профессиональной медицинской деятельности, сформировать правосознание и правовую культуру. Задачами дисциплины являются: - обучение основным положениям законов и иных нормативных правовых актов Российской Федерации, регулирующих отношения в области защиты прав потребителей, их толкованию; - обучение теоретическим знаниям об основных принципах, институтах, категориях и современном уровне развития защиты прав потребителей; - обучение определению объема предоставляемой информации об оказываемых услугах, критериях качества и безопасности товаров, работ, услуг, применению механизмов защиты прав потребителей на информацию, а также на качество и безопасность товаров, работ, услуг; - сформировать умение применять нормы гражданского, административного, предпринимательского и иных отраслей права при решении конкретных задач в области защиты прав потребителей; - выработка навыков применения законодательства о защите прав потребителей при осуществлении профессиональной деятельности. Трудоемкость курса составляет 72 часа. Курс состоит из 4 дидактических единиц, в рамках которых рассматривается 18 тем.
Prevention of children’s dental anomalies with anomalies of the oral mucosa. Early orthodontic treatment.
Authors: Bimbas E.S. , Shishmareva A.S.
Published: February 2023

Данный интерактивный обучающий модуль предназначен для врачей детских стоматологов, стоматологов общей практики, ортодонтов, будет полезен студентам стоматологического факультета, ординаторам. Цель данного модуля: повышение квалификации по специальности «Детская стоматология» и «Ортодонтия»; обновление знаний об этиологии и патогенезе зубочелюстных аномалий, связанных аномалиями слизистой оболочки полости рта; особенностях профилактики и раннего ортодонтического лечения детей с аномалиями слизистой оболочки полости рта; формулирование показаний (в том числе возрастных) и алгоритмов комплексного раннего ортодонтического лечения, с определением последовательности действий специалистов. Модуль содержит теоретические, практические и контролирующие материалы по профилактике и раннему ортодонтическому лечению ЗЧА у детей с аномалиями слизистой оболочки полости рта: аномалия прикрепления уздечки языка; уздечки верхней губы; нижней губы и преддверия полости рта; наличием травматического узла. Модуль позволит сформировать у обучающихся профессиональные навыки, научит объединять в единое целое разные методики профессиональной направленности; подготовит слушателей к использованию и применению полученных знаний и умений в профессиональной деятельности.
Electronic resource "Welcome to the UGMU team"
Authors: Neupokoeva Lidiya , Berthold Natalia , Shaforostova Anastasia
Published: February 2023

Разработанный электронный ресурс направлен на формирование у новых сотрудников УГМУ понимания о том, как организовано работа в университете
â€œShadow armyâ€ and military terrorism in 21st century Germany. The 2017 Der Tag X conspiracy
Authors: MuÃ±oz BolaÃ±os Roberto
Published: February 2023

En 2017 se descubriÃ³ en el seno de la Bundeswehr (Fuerzas Armadas) de Alemania una conspiraciÃ³n conocida como Tag X (DÃ­a X). Esta operaciÃ³n se habÃ­a articulado a partir del embriÃ³n de un posible â€œEjÃ©rcito en la sombraâ€, apoyado por organizaciones paramilitares, y su objetivo era tomar el poder mediante el uso de la violencia y el terror Der Tag X (El DÃ­a X). Esta dinÃ¡mica culminarÃ­a con la puesta en marcha de una â€œlimpieza Ã©tnicaâ€, la eliminaciÃ³n de los â€œenemigos internosâ€ y el establecimiento de un Estado autoritario. La tesis que mantenemos es que el origen de esta conspiraciÃ³n militar estÃ¡ Ã­ntimamente asociada a un conjunto de transformaciones polÃ­ticas, sociales, econÃ³micas y culturales que se han producido desde la Ãºltima dÃ©cada del siglo XX y que han creado una situaciÃ³n favorable para el ascenso de la extrema derecha en Alemania y â€œlegitimadoâ€ a militares de esta ideologÃ­a para intervenir en el proceso de toma de decisiones polÃ­ticas. Para desarrollar nuestro trabajo, hemos utilizado fundamentalmente dos tipos de fuentes. Por un lado, las primarias, formadas por los decretos sobre la tradiciÃ³n en la Bundeswehr, y las investigaciones parlamentarias y los informes de los servicios de inteligencia sobre el extremismo de derechas en la Bundeswehr. Por otro, las bibliogrÃ¡ficas y hemerogrÃ¡ficas, donde se recogen los principales acontecimientos vinculados a la conspiraciÃ³n de Der Tag X.
OperaciÃ³n leyenda negra: la negaciÃ³n del pensamiento espaÃ±ol en la raÃ­z de la FilosofÃ­a moderna. Black Legend Operation: the Denial of Spanish Thought at the root of Modern Philosophy
Authors: Holzenthal Nicole
Published: February 2023

El artÃ­culo demuestra que en el centro de Europa hubo un fuerte y profundo impacto de numerosas obras de pensamiento espaÃ±ol. Obras escritas en latÃ­n o espaÃ±ol en los siglos XVI y XVII, por escolÃ¡sticos o humanistas, cuyos planteamientos forman una raÃ­z importante de la filosofÃ­a moderna, ya que fueron recibidas muy abiertamente en muchos otros paÃ­ses europeos â€“ sobre todo, antes de su auge nacionalâ€“, sus ideas se adoptaron, manipularon y hasta plagiaron, y luego las obras se desecharon y su autorÃ­a quedÃ³ olvidada. Se plantea la hipÃ³tesis de que esos casos de absorciÃ³n y olvido de filosofÃ­a espaÃ±ola son sistemÃ¡ticos, y que tienen que ver con mecanismos propios de la leyenda negra, en una especie de operaciÃ³n de borrado o negaciÃ³n, para terminar en la actual ignorancia.