The Effect of Immersing Explants in Active Carbon Solution to Reduce Browning in Merbau (<i>Instia Bijuga </i>O.Ktze) Tissue Culture
Authors: Susanti Pinondanng M.H. , Widodo - , Kadam Nathan K. 
Published: April 2018

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classid="clsid:38481807-CA0E-42D2-BF39-B33AF135CC4D" id=ieooui></object> <mce:style><! st1\:*{behavior:url(#ieooui) } --> <!--[endif]--> <!--[if gte mso 10]> <mce:style><! /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} --> <!--[endif]--> <p class="MsoBodyText" style="margin-bottom: 0.0001pt; text-align: justify;"><span style="font-size: 10pt;" lang="EN-US">The research was aimed to observe the effect of active carbon solution at various levels of concentration and time before cultivated in Anderson media in order to reduce browning at merbau explants. The experiment was designed in Factorized Complete Random Design with concentration of active carbon solution as factor A (5 %, 10 %, 15 %) and immersing time as factor B (5 minutes, 10 minutes, 15 minutes), and was conducted in Kebun Bibit dan Laboratorium Kultur Jaringan &ndash; Waena, from 16 May 2005 to 8 August 2005.<span>&nbsp; </span>The result indicates that immersing explants in 15 % active carbon solution at 15 minutes immersing time before cultured in Anderson Media is effective to reduce browning in merbau tissue culture. In this treatment, 100% of explants and media are free from browning. Callus was formed in four weeks after cultured. However, during eight weeks observation time, all of the free-browning callus have not induced root and shoot growth yet.</span></p>