bluuebunny/crossref_metadata_2025 huggingface dataset:
Overdose prevention for injection drug users: Lessons learned from naloxone training and distribution programs in New York City
Authors: Piper Tinka Markham , Rudenstine Sasha , Stancliff Sharon , Sherman Susan , Nandi Vijay , Clear Allan , Galea Sandro
Published: January 2007

Background

Fatal heroin overdose is a significant cause of mortality for injection drug users (IDUs). Many of these deaths are preventable because opiate overdoses can be quickly and safely reversed through the injection of Naloxone [brand name Narcan], a prescription drug used to revive persons who have overdosed on heroin or other opioids. Currently, in several cities in the United States, drug users are being trained in naloxone administration and given naloxone for immediate and successful reversals of opiate overdoses. There has been very little formal description of the challenges faced in the development and implementation of large-scale IDU naloxone administration training and distribution programs and the lessons learned during this process.

Methods

During a one year period, over 1,000 participants were trained in SKOOP (Skills and Knowledge on Opiate Prevention) and received a prescription for naloxone by a medical doctor on site at a syringe exchange program (SEP) in New York City. Participants in SKOOP were over the age of 18, current participants of SEPs, and current or former drug users. We present details about program design and lessons learned during the development and implementation of SKOOP. Lessons learned described in the manuscript are collectively articulated by the evaluators and implementers of the project.

Results

There were six primary challenges and lessons learned in developing, implementing, and evaluating SKOOP. These include a) political climate surrounding naloxone distribution; b) extant prescription drug laws; c) initial low levels of recruitment into the program; d) development of participant appropriate training methodology; e) challenges in the design of a suitable formal evaluation; and f) evolution of program response to naloxone.

Conclusion

Other naloxone distribution programs may anticipate similar challenges to SKOOP and we identify mechanisms to address them. Strategies include being flexible in program planning and implementation, developing evaluation instruments for feasibility and simplicity, and responding to and incorporating feedback from participants.
Resistance of the target islet tissue to autoimmune destruction contributes to genetic susceptibility in Type 1 diabetes
Authors: Hill Natasha J , Stotland Aleksandr , Solomon Michelle , Secrest Patrick , Getzoff Elizabeth , Sarvetnick Nora
Published: January 2007

Type 1 diabetes occurs when self-reactive T lymphocytes destroy the insulin-producing islet β cells of the pancreas. The defects causing this disease have often been assumed to occur exclusively in the immune system. We present evidence that genetic variation at theIdd9diabetes susceptibility locus determines the resilience of the targets of autoimmunity, the islets, to destruction. Susceptible islets exhibit hyper-responsiveness to inflammatory cytokines resulting in enhanced cell death and increased expression of the death receptor Fas. Fas upregulation in β cells is mediated by TNFR2, and colocalization of TNFR2 with the adaptor TRAF2 in NOD β cells is altered.TNFR2lies within the candidateIdd9interval and the diabetes-associated variant contains a mutation adjacent to the TRAF2 binding site. A component of diabetes susceptibility may therefore be determined by the target of the autoimmune response, and protective TNFR2 signaling in islets inhibit early cytokine-induced damage required for the development of destructive autoimmunity.

Reviewers

This article was reviewed by Matthiasvon Herrath, HaraldVon Boehmer, and Ciriaco Piccirillo (nominated by Ethan Shevach).
Gene therapy with an improved doxycycline-regulated plasmid encoding a tumour necrosis factor-alpha inhibitor in experimental arthritis
Authors: Gould David , Yousaf Nasim , Fatah Rewas , Subang Maria Cristina , Chernajovsky Yuti
Published: January 2007

Inhibition of tumour necrosis factor (TNF)-alpha with biological molecules has proven an effective treatment for rheumatoid arthritis, achieving a 20% improvement in American College of Rheumatology score in up to 65% of patients. The main drawback to these and many other biological treatments has been their expense, which has precluded their widespread application. Biological molecules could alternatively be delivered by gene therapy as the encoding DNA. We have developed novel plasmid vectors termed pGTLMIK and pGTTMIK, from which luciferase and a dimeric TNF receptor II (dTNFR) are respectively expressed in a doxycycline (Dox)-regulated manner. Regulated expression of luciferase from the self-contained plasmid pGTLMIK was examined in vitro in a variety of cell lines and in vivo following intramuscular delivery with electroporation in DBA/1 mice. Dox-regulated expression of luciferase from pGTLMIK of approximately 1,000-fold was demonstrated in vitro, and efficient regulation was observed in vivo. The vector pGTTMIK encoding dTNFR was delivered by the same route with and without administration of Dox to mice with collagen-induced arthritis. When pGTTMIK was delivered after the onset of arthritis, progression of the disease in terms of both paw thickness and clinical score was inhibited when Dox was also administered. Vectors with similar regulation characteristics may be suitable for clinical application.
Diet and body constitution in relation to subgroups of breast cancer defined by tumour grade, proliferation and key cell cycle regulators
Authors: Borgquist Signe , Wirfält Elisabet , Jirström Karin , Anagnostaki Lola , Gullberg Bo , Berglund Göran , Manjer Jonas , Landberg Göran
Published: January 2007

Background

The general lack of clear associations between diet and breast cancer in epidemiological studies may partly be explained by the fact that breast cancer is a heterogeneous disease that may have disparate genetic associations and different aetiological bases.

Method

A total of 346 incident breast cancers in a prospective cohort of 17,035 women enrolled in the Malmö Diet and Cancer study (Sweden) were subcategorized according to conventional pathology parameters, proliferation and expression of key cell cycle regulators. Subcategories were compared with prediagnostic diet and body measurements using analysis of variance.

Results

A large hip circumference and high body mass index were associated with high grade tumours (P = 0.03 and 0.009, respectively), whereas low energy and unadjusted fat intakes were associated with high proliferation (P = 0.03 and 0.004, respectively). Low intakes of saturated, monounsaturated and polyunsaturated fatty acids were also associated with high proliferation (P = 0.02, 0.004 and 0.003, respectively). Low energy and unadjusted fat intakes were associated with cyclin D1 overexpression (P = 0.02 and 0.007, respectively), whereas cyclin E overexpression was positively correlated with fat intake. Oestrogen receptor status and expression of the tumour suppressor gene p27 were not associated with either diet or body constitution.

Conclusion

Low energy and low total fat (polyunsaturated fatty acids in particular) intakes, and high body mass index were associated with relatively more malignant breast tumours. Dietary behaviours and body constitution may be associated with specific types of breast cancer defined by conventional pathology parameters and cyclin D1 and cyclin E expression. Further studies including healthy control individuals are needed to confirm our results.
The Cdc20 (Fzy)/Cdh1-related protein, Cort, cooperates with Fzy in cyclin destruction and anaphase progression in meiosis I and II inDrosophila
Authors: Swan Andrew , Schüpbach Trudi
Published: January 2007

Meiosis is a highly specialized cell division that requires significant reorganization of the canonical cell-cycle machinery and the use of meiosis-specific cell-cycle regulators. The anaphase-promoting complex (APC)and a conserved APC adaptor, Cdc20 (also known as Fzy), are required for anaphase progression in mitotic cells. The APC has also been implicated in meiosis, although it is not yet understood how it mediates these non-canonical divisions. Cortex (Cort) is a diverged Fzy homologue that is expressed in the female germline of Drosophila, where it functions with the Cdk1-interacting protein Cks30A to drive anaphase in meiosis II. Here, we show that Cort functions together with the canonical mitotic APC adaptor Fzy to target the three mitotic cyclins (A, B and B3) for destruction in the egg and drive anaphase progression in both meiotic divisions. In addition to controlling cyclin destruction globally in the egg, Cort and Fzy appear to both be required for the local destruction of cyclin B on spindles. We find that cyclin B associates with spindle microtubules throughout meiosis I and meiosis II, and dissociates from the meiotic spindle in anaphase II. Fzy and Cort are required for this loss of cyclin B from the meiotic spindle. Our results lead to a model in which the germline-specific APCCortcooperates with the more general APCFzy, both locally on the meiotic spindle and globally in the egg cytoplasm, to target cyclins for destruction and drive progression through the two meiotic divisions.
Combinatorial signaling in the specification of primary pigment cells in theDrosophilaeye
Authors: Nagaraj Raghavendra , Banerjee Utpal
Published: January 2007

In the developing eye of Drosophila, the EGFR and Notch pathways integrate in a sequential, followed by a combinatorial, manner in the specification of cone-cell fate. Here, we demonstrate that the specification of primary pigment cells requires the reiterative use of the sequential integration between the EGFR and Notch pathways to regulate the spatiotemporal expression of Delta in pupal cone cells. The Notch signal from the cone cells then functions in the direct specification of primary pigment-cell fate. EGFR requirement in this process occurs indirectly through the regulation of Delta expression. Combined with previous work, these data show that unique combinations of only two pathways - Notch and EGFR - can specify at least five different cell types within the Drosophila eye.
Neuropilin asymmetry mediates a left-right difference in habenular connectivity
Authors: Kuan Yung-Shu , Yu Hung-Hsiang , Moens Cecilia B. , Halpern Marnie E.
Published: January 2007

The medial habenular nuclei of the zebrafish diencephalon, which lie bilateral to the pineal complex, exhibit left-right differences in their neuroanatomy, gene expression profiles and axonal projections to the unpaired midbrain target - the interpeduncular nucleus (IPN). Efferents from the left habenula terminate along the entire dorsoventral extent of the IPN, whereas axons from the right habenula project only to the ventral IPN. How this left-right difference in connectivity is established and the factors involved in differential target recognition are unknown. Prior to IPN innervation, we find that only the left habenula expresses the zebrafish homologue of Neuropilin1a (Nrp1a), a receptor for class III Semaphorins (Sema3s). Directional asymmetry of nrp1a expression relies on Nodal signaling and the presence of the left-sided parapineal organ. Loss of Nrp1a, through parapineal ablation or depletion by antisense morpholinos, prevents left habenular neurons from projecting to the dorsal IPN. Selective depletion of Sema3D, but not of other Sema family members, similarly disrupts innervation of the dorsal IPN. Conversely, Sema3D overexpression results in left habenular projections that extend to the dorsal IPN, as well as beyond the target. The results indicate that Sema3D acts in concert with Nrp1a to guide neurons on the left side of the brain to innervate the target nucleus differently than those on the right side.
Morphogenesis defects are associated with abnormal nervous system regeneration followingroboARNAi in planarians
Authors: Cebrià Francesc , Newmark Phillip A.
Published: January 2007

The process by which the proper pattern is restored to newly formed tissues during metazoan regeneration remains an open question. Here, we provide evidence that the nervous system plays a role in regulating morphogenesis during anterior regeneration in the planarian Schmidtea mediterranea. RNA interference (RNAi) knockdown of a planarian ortholog of the axon-guidance receptor roundabout (robo) leads to unexpected phenotypes during anterior regeneration, including the development of a supernumerary pharynx (the feeding organ of the animal) and the production of ectopic,dorsal outgrowths with cephalic identity. We show that Smed-roboARNAi knockdown disrupts nervous system structure during cephalic regeneration:the newly regenerated brain and ventral nerve cords do not re-establish proper connections. These neural defects precede, and are correlated with, the development of ectopic structures. We propose that, in the absence of proper connectivity between the cephalic ganglia and the ventral nerve cords,neurally derived signals promote the differentiation of pharyngeal and cephalic structures. Together with previous studies on regeneration in annelids and amphibians, these results suggest a conserved role of the nervous system in pattern formation during blastema-based regeneration.
A Survey of World Wide Web Use in Middle-Aged and Older Adults
Authors: Morrell Roger W. , Mayhorn Christopher B. , Bennett Joan
Published: January 2007

We conducted a survey to document World Wide Web use patterns in middle-aged (ages 40-59), young-old (ages 60-74), and old-old adults (ages 75-92). We conducted this survey of 550 adults 40 years old and over in southeastern Michigan, and the overall response rate was approximately 71%. The results suggested that (a) there are distinct age and demographic differences in individuals who use the Web; (b) middle-aged and older Web users are similar in their use patterns; (c) the two primary predictors for not using the Web are lack of access to a computer and lack of knowledge about the Web; (d) old-old adults have the least interest in using the Web compared with middle-aged and young-old adults; and (e) the primary content areas in learning how to use the Web are learning how to use electronic mail and accessing health information and information about traveling for pleasure. This research may serve as a preliminary attempt to ascertain the issues that must be faced in order to increase use of the World Wide Web in middle-aged and older adults.
An Empirical Comparison of Alternative Methodologies for the Evaluation of Configural Displays
Authors: Bennett Kevin B. , Payne Michael , Calcaterra Jeffery , Nittoli Bob
Published: January 2007

Two different methodologies (visual, memory) were used to evaluate alternative versions of the same configural display. One version (composite display) had several graphical design techniques applied, whereas the other version (baseline display) did not. Two types of information probes (high-level property, low-level data) were administered. When the displays were visible during completion of the probes (visual methodology), the display manipulation had the largest impact on performance (composite display associated with better performance); when the displays were not visible (memory methodology) the probe manipulation had the largest impact on performance (high-level probes associated with better performance). These results are interpreted in light of the mutually interacting constraints introduced by factors in display design, task requirements, and the participants' cognitive and perceptual capabilities/limitations. Implications for both the design and the evaluation of displays and interfaces in general are discussed. Actual or potential applications of this research include design techniques for improving the quality of graphic displays and methodological insights for interpreting previous research and guiding future experimentation.
Putting It All Together: Improving Display Integration in Ecological Displays
Authors: Burns Catherine M.
Published: January 2007

Computer displays are being designed for increasingly larger industrial systems. As the application domain scales up, maintaining integration across different kinds of views becomes more challenging. This paper presents the results of a study of three different approaches to integration based on the spatial and temporal proximity of related information objects. The domain used for evaluation was a simulation of an industry-scale conventional power plant. All three displays were ecological displays developed using an abstraction hierarchy analysis. Views were integrated in a high-space/low-time, low-space/high-time, and high-space/high-time integration of means-end related objects. During a fault detection and diagnosis task, it as found that a low level of integration, high-space/low-time, provided the fastest fault detection time. However, the most integrated condition, high-space/high-time, resulted in the fastest and most accurate fault diagnosis performance. Actual or potential applications of this research include computer displays for large-scale systems such as network management or process control, for which problem solving is critical and integration must be maintained.
Assessment of the Relationship between Box Weight and Trunk Kinematics: Does a Reduction in Box Weight Necessarily Correspond to a Decrease in Spinal Loading?
Authors: Davis Kermit G. , Marras William S.
Published: January 2007

Typically, the simplest and most cost-efficient ergonomic solution to offset the rising costs of low back injuries is to reduce the box weight that is lifted. However, there is limited research on how a worker interacts with the box. In the present study, we quantify the utility of reducing the weight that is lifted---specifically, how changes in the box weight affect trunk kinematics, trunk moments, and ultimately, spinal loads. In the experiment, 15 participants lifted a variety of box weights (from 9.1 to 41.7 kg) from knee height, carried it a distance of 5 feet (1.5 m), and placed it on a shelf at elbow height. For the lower weights, small increases in box weight (3-9 kg) were offset by the trunk dynamics (sagittal velocity), resulting in no difference in spinal loads. At the same time, spinal loads were found to be significantly higher for weights above 25 kg. Thus, when making ergonomic changes (reduction of box weight), it is important to consider how workers will interact with the box. These results indicate that purely weight-based ergonomic controls might not sufficiently reduce the risk of low back disorders. Furthermore, this study provides additional evidence of the utility of using more complex spinal load models (dynamic, multiple muscle models) when evaluating highly dynamic and complex tasks.
Continuous Assessment of Back Stress (CABS): A New Method to Quantify Low-Back Stress in Jobs with Variable Biomechanical Demands
Authors: Mirka Gary A. , Kelaher Daniel P. , Nay D. Todd , Lawrence Brad M.
Published: January 2007

Jobs with a high degree of variability in manual materials handling requirements expose limitations in current low-back injury risk assessment tools and emphasize the need for a probabilistic representation of the biomechanical stress in order to quantify both acute and cumulative trauma risk. We developed a hybrid assessment methodology that employs established assessment tools and then represents their evaluations in a way that emphasizes the distributions of biomechanical stress. Construction work activities in the home building industry were evaluated because of the high degree of variability in the manual material handling requirements. Each task was evaluated using the Revised NIOSH Lifting Equation, The University of Michigan Three- Dimensional Static Strength Prediction Program™, and the Ohio State University Lumbar Motion Monitor Model. The output from each model was presented as time-weighted histograms of low-back stress, and the assessments were compared. The results showed considerable differences in what were considered high-risk activities, indicating that these 3 assessment tools consider the risk of low-back injury from different perspectives. The time-weighted distribution aspect of this methodology also contributed vital information toward the identification of high-risk activities. These results illustrate the necessity for more advanced low-back injury risk assessment techniques for jobs with highly variable manual materials handling requirements.
Team Mental Models: Techniques, Methods, and Analytic Approaches
Authors: Langan-Fox Janice , Code Sharon , Langfield-Smith Kim
Published: January 2007

Effective team functioning requires the existence of a shared or team mental model among members of a team. However, the best method for measuring team mental models is unclear. Methods reported vary in terms of how mental model content is elicited and analyzed or represented. We review the strengths and weaknesses of various methods that have been used to elicit, represent, and analyze individual and team mental models and provide recommendations for method selection and development. We describe the nature of mental models and review techniques that have been used to elicit and represent them. We focus on a case study on selecting a method to examine team mental models in industry. The processes involved in the selection and development of an appropriate method for eliciting, representing, and analyzing team mental models are described. The criteria for method selection were (a) applicability to the problem under investigation; (b) practical considerations---suitability for collecting data from the targeted research sample; and (c) theoretical rationale---the assumption that associative networks in memory are a basis for the development of mental models. We provide an evaluation of the method matched to the research problem and make recommendations for future research. The practical applications of this research include the provision of a technique for analyzing team mental models in organizations, the development of methods and processes for eliciting a mental model from research participants in their normal work environment, and a survey of available methodologies for mental model research.
Isoperformance and Personnel Decisions
Authors: Jones Marshall B.
Published: January 2007

Isoperformance curves are contour lines or surfaces that trace all combinations of two or more determinants that suffice to produce a specified level of performance? hence the term isoperformance. When the determinants are aptitude and training time or time on the job, an isoperformance curve allows one to identify individuals who have sufficient aptitude for the work that they can be expected to reach a specified standard of performance in a specified length of time. This paper discusses isoperformance in the personnel selection and classification literature and then develops and illustrates the uses of isoperformance curves in personnel decisions. The idea of aptitude sensitivity is introduced, and the relationship between this idea and predictive validity is explained. The paper also indicates how isoperformance can be used to detail trade-offs among training time, the minimum qualifying score, attrition rate, quotas, and the specified level of criterion performance. Isoperformance finds direct application in the multiple-job assignment problem, exemplified by the choice of which recruit to send to which training school in the military services.
Effect of Aftermarket Automobile Window Tinting Films on Driver Vision
Authors: LaMotte James , Ridder William , Yeung Karen , De Land Paul
Published: January 2007

This study was conducted to determine the level of automobile window tint that causes a significant reduction of vision for automobile drivers. Contrast sensitivity was measured on 20 participants, of whom 10 were age 20 to 29 years and 10 were age 60 to 69 years, through a stock automobile window (control) and two windows darkened with plastic film. For the younger drivers, a car window with 37% transmittance did not significantly reduce contrast sensitivity, but a darker tint of 18% transmittance reduced contrast sensitivity at higher spatial frequencies. For the older drivers, a tint of 37% transmittance significantly reduced mid-to- high spatial frequency contrast sensitivity. The typical state standard (no tint with less than 35% transmittance) would thus seem to be appropriate for younger drivers; however, further examination of the standard may be necessary in regard to older drivers. Actual or potential applications of this research include guidelines and regulations regarding tinting of automobile windows.
Visual Display Height
Authors: Burgess-Limerick Robin , Mon-Williams Mark , Coppard Vanessa L.
Published: January 2007

We examined the influence of backrest inclination and vergence demand on the posture and gaze angle that workers adopt to view visual targets placed in different vertical locations. In the study, 12 participants viewed a small video monitor placed in 7 locations around a 0.65-m radius arc (from 65° below to 30° above horizontal eye height). Trunk posture was manipulated by changing the backrest inclination of an adjustable chair. Vergence demand was manipulated by using ophthalmic lenses and prisms to mimic the visual consequences of varying target distance. Changes in vertical target location caused large changes in atlanto-occipital posture and gaze angle. Cervical posture was altered to a lesser extent by changes in vertical target location. Participants compensated for changes in backrest inclination by changing cervical posture, though they did not significantly alter atlanto-occipital posture and gaze angle. The posture adopted to view any target represents a compromise between visual and musculoskeletal demands. These results provide support for the argument that the optimal location of visual targets is at least 15° below horizontal eye level. Actual or potential applications of this work include the layout of computer workstations and the viewing of displays from a seated posture.
The Utility of Event-Based Knowledge Elicitation
Authors: Fowlkes Jennifer E. , Salas Eduardo , Baker David P. , Cannon-Bowers Janis A. , Stout Renée J.
Published: January 2007

The purpose of this investigation was to describe and evaluate an event-based knowledge elicitation technique. With this approach experts are provided with deliberate and controlled job situations, allowing investigation of specific task aspects and the comparison of expert responses. For this effort a videotape was developed showing an instructor pilot and student conducting a training mission. Various job situations were depicted in the video to gather information pertinent to understanding team situational awareness. The videotape was shown to 10 instructors and 10 student aviators in the community, and responses to the videotape were collected using a questionnaire at predetermined stop points. Consistent with expectations, the results showed that more experienced respondents (i.e., instructors) identified a richer database of cues and were more likely than students to identify strategies for responding to the situations depicted, providing some empirical evidence for the validity of the event-based technique. This method may serve as a useful knowledge elicitation technique, especially in the later stages of a job analysis when focused information is sought.
A Framework for Epistemological Analysis in Empirical (Laboratory and Field) Studies
Authors: Xiao Yan , Vicente Kim J.
Published: January 2007

In their search for generalizable behavioral patterns and design principles, cognitive field researchers should reflect on the epistemological limitations of empirical studies. In this paper we describe a framework for epistemological analysis that can help serve this purpose and discuss its application to two prototypical cases of cognitive engineering research: laboratory experiments and field studies. The framework examines two, often implicit, processes in empirical research: the abstraction from empirical data and the substantiation of theoretical constructs and principles. By explicitly considering these two processes in several systematic steps, we can gain appreciation for the epistemological contribution of empirical studies to cognitive engineering research. The framework and its application also provide guidance to such important issues as generalizability of results and external validity. Possible applications of this research include providing guidance to researchers and practitioners in evaluating design principles or conducting field studies.
A Comparison of Seven Visual Fatigue Assessment Techniques In Three Data-Acquisition VDT Tasks
Authors: Chi Chia-Fen , Lin Fang-Tsan
Published: January 2007

We compared 7 methods of measuring visual fatigue - accommodation power, visual acuity, pupil diameter, critical fusion frequency (CFF), eye movement velocity, subjective rating of visual fatigue, and task performance - for their sensitivity to visual load. In the experiment, 10 participants performed a monitoring task at 2 viewing distances, read articles under 2 levels of screen contrast, and tracked visual targets at 2 different speeds. The same measurement techniques, excluding pupil diameter and eye movement velocity, were compared by extending the task time from 20 to 60 min with the same VDT tasks to test for possible improvement in sensitivity. The results indicated that sensitivities of accommodation power, visual acuity, and CFF were greatly improved by a longer task period, but these 3 measurement techniques did not distinguish among tasks. Pupil diameter, eye movement velocity, and subjective rating of visual fatigue were sensitive in differentiating tracking from reading and monitoring tasks. Eye movement velocity and subjective rating were sensitive to the changes in target velocity of the tracking task. Although task performance was not directly comparable to other measurement techniques, it helped to ensure that participants maintained the same performance level by devoting more resources to the highload conditions. Actual or potential applications of this research include using some of these assessment techniques for the design of adaptive displays.
Context Effects in Subjective Mental Workload Ratings
Authors: Colle Herbert A. , Reid Gary B.
Published: January 2007

The impact of performance context on subjective mental workload ratings was assessed with the Subjective Workload Assessment Technique (SWAT) and the NASA Task Load Index (TLX). In Experiment 1, a strong context effect was demonstrated. A low range of task difficulty produced considerably higher ratings on a common set of difficulty levels than did a high range of task difficulty. In Experiment 2, increasing the participants′ range of experiences during practice eliminated the context effect. We recommend that methods for standardizing context, such as providing experience with the complete difficulty range, be developed for subjective mental workload evaluations. Actual or potential applications of this research include providing methodologies for controlling context effects in practical assessments of mental workload to increase the validity of subjective measures.
Individual Differences in Accident Liability: A Review and Integrative Approach
Authors: Lawton Rebecca , Parker Dianne
Published: January 2007

This paper reviews research since 1970 on the relationship between accident liability and individual differences, focusing specifically on accidents at work. The history of research into accident liability and the methodological problems associated with the research are considered. The review goes on to examine work on the impact of personality factors, cognitive factors, and social factors on the likelihood of accident involvement at work. We suggest that research into individual differences in accident liability should consider two possible routes to accident involvement via errors and/or violations. Although errors are predominantly associated with cognitive factors, violations have their origins in social psychological factors. We also consider the role of stress in mediating the personality-accident association. It is contended that individuals differ in their reactions to stress, so that although some respond by an increase in risk-taking behavior, the effect on others is to increase the likelihood of suboptimal performance in terms of information processing. Actual or potential applications of this research include the development of a more sophisticated model of individual differences in accident liability, which should be useful to organizations attempting to promote safety.
Evaluation of Supermarket Bagging Using a Wrist Motion Monitor
Authors: Estill Cheryl Fairfield , Kroemer K. H. E.
Published: January 2007

The supermarket industry has one of the highest numbers of repeated trauma illnesses. Checkout departments have a rate of musculoskeletal injuries 2 to 3 times higher than that of other supermarket departments. The primary objective of this study was to quantify the wrist motions required to bag groceries using a wrist motion monitor. The wrist motions included deviations, velocities, and accelerations for flexion-extension, radial-ulnar, and pronation-supination directions. The independent variables were handle type and object location. Objects with finger-thumb couplings required more extreme pronations, greater wrist velocities for pronation-supination deviations, and greater wrist accelerations for pronation-supination deviations than did other objects. Objects with 10-cm hand couplings required more extreme flexion, larger ranges of movement for radial-ulnar deviations and pronation-supination deviations, and greater wrist velocities in the radial-ulnar and pronation-supination directions than did 5-cm objects. The right and front locations required more extreme deviations than did the left and back locations. Because finger-thumb and 10-cm hand couplings require larger wrist deviations and greater velocities, these objects may pose a greater risk of developing cumulative trauma disorders to the bagger. Potential applications of this research include engineering design of grocery packaging and supermarket bagging workstations.
Observation versus Hands-on Practice of Complex Skills in Dyadic, Triadic, and Tetradic Training-Teams
Authors: Shebilske Wayne L. , Jordan Jeffrey A. , Goettl Barry P. , Paulus Leigh E.
Published: January 2007

We saved trainer time and resources by reducing hands-on practice in training-team protocols without reducing computer-based learning. During 1-h sessions, young adults learned Space Fortress, a video task that has been employed during pilot training. Observers (Experiment 1) learned more in 3 sessions than did test-only trainees. Individuals, dyads, triads, and tetrads (Experiment 2) learned in 3 sessions with no differences in learning or interaction between learning and protocol. Individuals, dyads, and tetrads (Experiment 3) learned in 10 sessions with no differences in learning or interaction between learning and protocol. As predicted by social learning theory, observational learning seems to compensate for hands-on practice efficiently and effectively. Applications of this research have been developed for computer-based group training of airline and air force pilots.
How Automatic Is Manual Gear Shifting?
Authors: Shinar David , Meir Meir , Ben-Shoham Israel
Published: January 2007

Manual gear shifting is often used as an example of an automated (vs. controlled) process in driving. The present study provided an empirical evaluation of this assumption by evaluating sign detection and recall performance of novice and experienced drivers driving manual shift and automatic transmission cars in a downtown area requiring frequent gear shifting. The results showed that manual gear shifting significantly impaired sign detection performance of novice drivers using manual gears compared with novice drivers using an automatic transmission, whereas no such differences existed between the two transmission types for experienced drivers. The results clearly demonstrate that manual gear shifting is a complex psychomotor skill that is not easily (or quickly) automated and that until it becomes automated, it is an attention-demanding task that may impair other monitoring aspects of driving performance. Actual or potential applications of this research include a reevaluation of the learning process in driving and the need for phased instruction in driving from automatic gears to manual gears.
Information Structure and the Relative Efficacy of Tables and Graphs
Authors: Meyer Joachim , Shamo Marcia Kuskin , Gopher Daniel
Published: January 2007

Users and system designers often prefer to display information with graphs rather than with tables. However, empirical studies that compared task performance with the two display types frequently revealed either an advantage of tables over graphs or no differences between the displays. This apparent contradiction may result from previous studies in which the importance of the structure that usually exists in displayed information is overlooked. We predict that graphic displays will have an advantage over tables when the displayed information has structure and when this structure is relevant for the task. These conditions generally exist in the actual use of information displays, but have seldom been assessed in experiments. In the present study participants in an experiment performed an information extraction task and a prediction task with unstructured or structured data and with different levels of prior information about the structure. The results showed that the information structure and prior knowledge about the existence of structure affected the advantage of graphic displays over tables when task performance depended on the use of structure. Existing approaches to the study of displays were analyzed in view of these findings. Actual or potential applications of this research include the development of better displays for process control and decision support and better operator training programs.
Aβ Oligomer-Induced Aberrations in Synapse Composition, Shape, and Density Provide a Molecular Basis for Loss of Connectivity in Alzheimer's Disease
Authors: Lacor Pascale N. , Buniel Maria C. , Furlow Paul W. , Sanz Clemente Antonio , Velasco Pauline T. , Wood Margaret , Viola Kirsten L. , Klein William L.
Published: January 2007

The basis for memory loss in early Alzheimer's disease (AD) seems likely to involve synaptic damage caused by soluble Aβ-derived oligomers (ADDLs). ADDLs have been shown to build up in the brain and CSF of AD patients and are known to interfere with mechanisms of synaptic plasticity, acting as gain-of-function ligands that attach to synapses. Because of the correlation between AD dementia and synaptic degeneration, we investigated here the ability of ADDLs to affect synapse composition, structure, and abundance. Using highly differentiated cultures of hippocampal neurons, a preferred model for studies of synapse cell biology, we found that ADDLs bound to neurons with specificity, attaching to presumed excitatory pyramidal neurons but not GABAergic neurons. Fractionation of ADDLs bound to forebrain synaptosomes showed association with postsynaptic density complexes containing NMDA receptors, consistent with observed attachment of ADDLs to dendritic spines. During binding to hippocampal neurons, ADDLs promoted a rapid decrease in membrane expression of memory-related receptors (NMDA and EphB2). Continued exposure resulted in abnormal spine morphology, with induction of long thin spines reminiscent of the morphology found in mental retardation, deafferentation, and prionoses. Ultimately, ADDLs caused a significant decrease in spine density. Synaptic deterioration, which was accompanied by decreased levels of the spine cytoskeletal protein drebrin, was blocked by the Alzheimer's therapeutic drug Namenda. The observed disruption of dendritic spines links ADDLs to a major facet of AD pathology, providing strong evidence that ADDLs in AD brain cause neuropil damage believed to underlie dementia.
Bim Is Elevated in Alzheimer's Disease Neurons and Is Required for β-Amyloid-Induced Neuronal Apoptosis
Authors: Biswas Subhas C. , Shi Yijie , Vonsattel Jean-Paul G. , Leung Conrad L. , Troy Carol M. , Greene Lloyd A.
Published: January 2007

The molecules that mediate neuron death in Alzheimer's disease (AD) are largely unknown. We report that β-amyloid (Aβ), a death-promoting peptide implicated in the pathophysiology of AD, induces the proapoptotic protein Bcl-2 interacting mediator of cell death (Bim) in cultured hippocampal and cortical neurons. We further find that Bim is an essential mediator of Aβ-induced neurotoxicity. Our examination of postmortem AD human brains additionally reveals upregulation of Bim in vulnerable entorhinal cortical neurons, but not in cerebellum, a region usually unaffected by AD. Accumulating evidence links inappropriate induction/activation of cell cycle-related proteins to AD, but their roles in the disease have been unclear. We find that the cell cycle molecule cyclin-dependent kinase 4 (cdk4) and its downstream effector B-myb, are required for Aβ-dependent Bim induction and death in cultured neurons. Moreover, neurons that overexpress Bim in AD brains also show elevated levels of the cell cycle-related proteins cdk4 and phospho-Rb. Our observations indicate that Bim is a proapoptotic effector of Aβ and of dysregulated cell cycle proteins in AD and identify both Bim and cell cycle elements as potential therapeutic targets.
ERG Conductance Expression Modulates the Excitability of Ventral Horn GABAergic Interneurons That Control Rhythmic Oscillations in the Developing Mouse Spinal Cord
Authors: Furlan Francesco , Taccola Giuliano , Grandolfo Micaela , Guasti Leonardo , Arcangeli Annarosa , Nistri Andrea , Ballerini Laura
Published: January 2007

During antenatal development, the operation and maturation of mammalian spinal networks strongly depend on the activity of ventral horn GABAergic interneurons that mediate excitation first and inhibition later. Although the functional consequence of GABA actions may depend on maturational processes in target neurons, it is also likely that evolving changes in GABAergic transmission require fine-tuning in GABA release, probably via certain intrinsic mechanisms regulating GABAergic neuron excitability at different embryonic stages. Nevertheless, it has not been possible, to date, to identify certain ionic conductances upregulated or downregulated before birth in such cells. By using an experimental model with either mouse organotypic spinal cultures or isolated spinal cord preparations, the present study examined the role of the ERG current (IK(ERG)), a potassium conductance expressed by developing, GABA-immunoreactive spinal neurons. In organotypic cultures, only ventral interneurons with fast adaptation and GABA immunoreactivity, and only after 1 week in culture, were transformed into high-frequency bursters by E4031, a selective inhibitor ofIK(ERG)that also prolonged and made more regular spontaneous bursts. In the isolated spinal cord in which GABA immunoreactivity andm-ergmRNA were colocalized in interneurons, ventral root rhythms evoked by NMDA plus 5-hydroxytryptamine were stabilized and synchronized by E4031. All of these effects were lost after 2 weeks in culture or before birth in coincidence with decreasedm-ergexpression. These data suggest that, during an early stage of spinal cord development, the excitability of GABAergic ventral interneurons important for circuit maturation depended, at least in part, on the function ofIK(ERG).
Phasic Dopamine Release Evoked by Abused Substances Requires Cannabinoid Receptor Activation
Authors: Cheer Joseph F. , Wassum Kate M. , Sombers Leslie A. , Heien Michael L. A. V. , Ariansen Jennifer L. , Aragona Brandon J. , Phillips Paul E. M. , Wightman R. Mark
Published: January 2007

Transient surges of dopamine in the nucleus accumbens are associated with drug seeking. Using a voltammetric sensor with high temporal and spatial resolution, we demonstrate differences in the temporal profile of dopamine concentration transients caused by acute doses of nicotine, ethanol, and cocaine in the nucleus accumbens shell of freely moving rats. Despite differential release dynamics, all drug effects are uniformly inhibited by administration of rimonabant, a cannabinoid receptor (CB1) antagonist, suggesting that an increase in endocannabinoid tone facilitates the effects of commonly abused drugs on subsecond dopamine release. These time-resolved chemical measurements provide unique insight into the neurobiological effectiveness of rimonabant in treating addictive disorders.
N-Terminally Deleted Forms of the Prion Protein Activate Both Bax-Dependent and Bax-Independent Neurotoxic Pathways
Authors: Li Aimin , Barmada Sami J. , Roth Kevin A. , Harris David A.
Published: January 2007

Transgenic (Tg) mice expressing prion protein (PrP) with a deletion of the flexible, N-terminal tail encompassing residues 32–134 spontaneously develop ataxia, degeneration of cerebellar granule cells, and vacuolation of white matter in the brain and spinal cord, resulting in death by 3 months of age. These abnormalities are completely abrogated by coexpression of wild-type PrP from a single copy of the endogenousPrn-pgene. A similar but much more severe phenotype is seen in transgenic mice expressing PrP deleted for a conserved block of 21 amino acids (residues 105–125) within the N-terminal tail. The latter animals die within 1 week of birth in the absence of endogenous PrP, and fivefold overexpression of wild-type PrP is required to delay death beyond 1 year. To define the cellular pathways mediating the neurotoxicity of PrPΔ32–134 and PrPΔ105–125, we analyzed the effect of genetically deleting the proapoptotic protein Bax in mice expressing these neurotoxic forms of PrP. We find that Bax deletion in Tg(PrPΔ32–134) mice delays the development of clinical illness and slows apoptosis of cerebellar granule cells but has no effect on white matter degeneration. In contrast, Bax deletion has no effect on the clinical or neuropathological phenotype of Tg(Δ105–125) mice. Our results indicate that Bax-related pathways mediate the initial neurotoxic actions of PrPΔ32–134 but that neurodegeneration induced by this protein as well as by PrPΔ105–125 also involves Bax-independent pathways.
The Induction Levels of Heat Shock Protein 70 Differentiate the Vulnerabilities to Mutant Huntingtin among Neuronal Subtypes
Authors: Tagawa Kazuhiko , Marubuchi Shigeki , Qi Mei-Ling , Enokido Yasushi , Tamura Takuya , Inagaki Reina , Murata Miho , Kanazawa Ichiro , Wanker Erich E. , Okazawa Hitoshi
Published: January 2007

The reason why vulnerabilities to mutant polyglutamine (polyQ) proteins are different among neuronal subtypes is mostly unknown. In this study, we compared the gene expression profiles of three types of primary neurons expressing huntingtin (htt) or ataxin-1. We found that heat shock protein 70 (hsp70), a well known chaperone molecule protecting neurons in the polyQ pathology, was dramatically upregulated only by mutant htt and selectively in the granule cells of the cerebellum. Granule cells, which are insensitive to degeneration in the human Huntington's disease (HD) pathology, lost their resistance by suppressinghsp70with siRNA, whereas cortical neurons, affected in human HD, gained resistance by overexpressinghsp70. This indicates that induction levels ofhsp70are a critical factor for determining vulnerabilities to mutant htt among neuronal subtypes. CAT (chloramphenicol acetyltransferase) assays showed that CBF (CCAAT box binding factor, CCAAT/enhancer binding protein ζ) activated, but p53 repressed transcription of thehsp70gene in granule cells. Basal and mutant htt-induced expression levels of p53 were remarkably lower in granule cells than in cortical neurons, suggesting that different magnitudes of p53 are linked to distinct induction levels ofhsp70. Surprisingly, however, heat shock factor 1 was not activated in granule cells by mutant htt. Collectively, different levels ofhsp70among neuronal subtypes might be involved in selective neuronal death in the HD pathology.