bluuebunny/crossref_metadata_2025 huggingface dataset:
Robotic Surgery: Is There a Possibility of Increasing Its Application in Pediatric Settings? A Single-Center Experience
Authors: Bindi Edoardo , Todesco Camilla , Nino Fabiano , Torino Giovanni , Gentilucci Gianluca , Cobellis Giovanni
Published: July 2022

Introduction: Robotic surgery has shown explicit benefits and advantages in adults, but it is not yet strongly established in the pediatric population, even though its popularity is increasing, especially in the urologic field. Materials and methods: In this article we present our experience with the Da Vinci System (SI first and XI nowadays) at our pediatric institution in order to analyze our progress over the years. We considered all patients from the start of the robotic surgery program in 2016 until the end of 2021, dividing them into general abdominal surgery and genitourinary surgery. Analyzed data were the patient’s demographic, details of surgery, and intra and post-operative complications. Results: The total number of patients (pts) included in this study was 76, of whom 40 (52%) were male and 36 (48%) were female. The mean age at surgery was 90.9 months (range 10–207 months), and the mean weight at surgery was 29.3 kg (range 9.5–68 kg). There were 18 general abdominal robotic surgeries and 58 genitourinary robotic surgeries performed. The most performed surgeries in these two categories were fundoplication for gastro-oesophageal reflux disease (11%) and Anderson–Hynes pyeloureteroplasty. The mean operative time was 224.2 min (range 72–530 min): the mean times in the two groups (general abdominal surgery and genitourinary surgery) were 165 min (range 84–204 min) and 194 min (range 95–360 min), respectively. A total of four (5%) minor complications were reported. The total conversions were two (2.6%) and the mortality rate was 0%. Conclusions: Pediatric robotic surgery is a field of considerable interest and it is rapidly expanding. In our experience, it is evident how the learning curve has increased gradually, but steadily, allowing us to advance from standardized surgery, such as fundoplication and pieloplasty, towards a more technically complex one, achieving the same good results. We believe that robotic surgery is very respectful of tissues and feasible, especially for reconstructive surgery. For these reasons, it could become of common use also in the pediatric population, overcoming impediments such as excessive cost and the lack of pediatric instruments, in order to be able to treat children with a progressively lower age and weight.
Associations between Self-Objectification and Lifestyle Habits in a Large Sample of Adolescents
Authors: Baceviciene Migle , Jankauskiene Rasa , Trinkuniene Laima
Published: July 2022

The aim of the present study was to test associations between self-objectification and health-related lifestyle habits in a large sample of adolescents. In 2019–2020, a large sample of adolescents (n = 1402, 40.4% boys) participated in the survey and, as a part of a larger study, filled in questionnaires on self-objectification and lifestyle. Self-objectification was assessed using the Self-Objectification Questionnaire (SOQ). The lifestyle questionnaire had three batteries: eating attitudes and behaviors, dietary habits, and questions on harmful behaviors together with time spending patterns: sedentary lifestyle and physical activity. The ages ranged from 15–19 years with a mean age of 16.97 ± 0.46 years. Self-objectification (SO) was associated with a less healthy lifestyle: more frequent dieting and unhealthy eating habits in girls, skipping breakfast, and a lower number of meals per day. Adolescent boys and girls with higher SO demonstrated more frequent smoking and alcohol consumption until they felt dizzy and more frequent consumption of soft drinks, energy drinks, and fast foods. Girls with higher SO demonstrated lower perceived physical activity and longer duration of internet browsing for non-educational purposes. The results of the present study inform healthy lifestyle promotion programs for adolescents, suggesting that including psychoeducation about self-objectification, body functionality, and positive embodiment might be an effective strategy.
Probiotic Potential of Clostridium spp.—Advantages and Doubts
Authors: Grenda Tomasz , Grenda Anna , Domaradzki Piotr , Krawczyk Paweł , Kwiatek Krzysztof
Published: July 2022

Clostridium spp. is a large genus of obligate anaerobes and is an extremely heterogeneous group of bacteria that can be classified into 19 clusters. Genetic analyses based on the next-generation sequencing of 16S rRNA genes and metagenome analyses conducted on human feces, mucosal biopsies, and luminal content have shown that the three main groups of strict extremophile anaerobes present in the intestines are Clostridium cluster IV (also known as the Clostridium leptum group), Clostridium cluster XIVa (also known as the Clostridium coccoides group) and Bacteroides. In addition to the mentioned clusters, some C. butyricum strains are also considered beneficial for human health. Moreover, this bacterium has been widely used as a probiotic in Asia (particularly in Japan, Korea, and China). The mentioned commensal Clostridia are involved in the regulation and maintenance of all intestinal functions. In the literature, the development processes of new therapies are described based on commensal Clostridia activity. In addition, some Clostridia are associated with pathogenic processes. Some C. butyricum strains detected in stool samples are involved in botulism cases and have also been implicated in severe diseases such as infant botulism and necrotizing enterocolitis in preterm neonates. The aim of this study is to review reports on the possibility of using Clostridium strains as probiotics, consider their positive impact on human health, and identify the risks associated with the expression of their pathogenic properties.
All-Dielectric Refractive Index Sensor Based on Multiple Fano Resonance with High Sensitivity in the Long-Wave Infrared Region
Authors: Wang Kai , Wang Yansong , Wang Xiaokun , Wang Xiaoyi , Gao Jinsong , Yang Haigui
Published: July 2022

In this paper, we propose an all-dielectric metamaterials structure which contains four asymmetric square holes in the unit cell to design a high-sensitivity refractive index sensor in the long-wave infrared region. Theoretical analysis of the electromagnetic field distributions shows that the four transmission dips originate from magnetic dipole, electric quadrupole and Toroidal dipole. And its position can be tuned by adjusting different geometric parameters, which can optimize the structure to obtain a narrower linewidth to improve the performance of the sensor. Finally, we evaluate the performance of the structure as refractive index sensor by changing the refractive index of the tested substrate. The results show the refractive index sensor has high sensitivity in the long-wave infrared region: the highest sensitivity is 2803 nm/RIU and the figure of merit will reach up to 350.
Analyses of Tool Wear and Chip Type for Different Coated Carbide Inserts in Turning Hardened 1.6582 Steel
Authors: Šramhauser Karel , Náprstková Nataša , Sviantek Jan , Stančeková Dana , Van Tuong Nguyen , Novotný Jan
Published: July 2022

This article deals with the comparative wear of CNMG-coated sintered carbide indexable cutting inserts by several manufacturers when turning 1.6582 steel hardened to 40–44 HRC. The main goal will be to demonstrate the different course of wear by testing seemingly identical inserts for engineering companies, showing the connection between the course of wear and the production of waste in the form of chips. The monitored type of wear was the flank wear VBmax of a cutting tool depending on the length of the machining time. Additionally, the effect of cutting tool wear on chip production, i.e., their quantity, shape and size, was monitored. For the purpose of mutual comparison, uniform cutting conditions were chosen on the basis of previous experiments, ensuring the stability of the given cutting process. For flank wear analysis, optical and electron microscopes were used. Meanwhile, a portable roughness tester was used to analyze the roughness of the machined surface. It was found that the quality of the interconnected coating layers has a perceptible influence on wear rate of the evaluated cutting inserts. The relation between the degree and characteristics of wear on the one hand, and the properties of the produced chips that identify the effect of the difference in the deposition layers using EDX/SEM analysis on the other, foreshadows the importance of choose between products with similarly declared properties was found. The current assessment of cutting tool wear and the evaluation of the chip produced has significant economic potential for manufacturing companies seeking to minimize costs.
Chebfun Solutions to a Class of 1D Singular and Nonlinear Boundary Value Problems
Authors: Gheorghiu Călin-Ioan
Published: July 2022

The Chebyshev collocation method implemented in Chebfun is used in order to solve a class of second order one-dimensional singular and genuinely nonlinear boundary value problems. Efforts to solve these problems with conventional ChC have generally failed, and the outcomes obtained by finite differences or finite elements are seldom satisfactory. We try to fix this situation using the new Chebfun programming environment. However, for tough problems, we have to loosen the default Chebfun tolerance in Newton’s solver as the ChC runs into trouble with ill-conditioning of the spectral differentiation matrices. Although in such cases the convergence is not quadratic, the Newton updates decrease monotonically. This fact, along with the decreasing behaviour of Chebyshev coefficients of solutions, suggests that the outcomes are trustworthy, i.e., the collocation method has exponential (geometric) rate of convergence or at least an algebraic rate. We consider first a set of problems that have exact solutions or prime integrals and then another set of benchmark problems that do not possess these properties. Actually, for each test problem carried out we have determined how the Chebfun solution converges, its length, the accuracy of the Newton method and especially how well the numerical results overlap with the analytical ones (existence and uniqueness).
In Vitro and Electrochemical Characterization of Laser-Cladded Ti-Nb-Ta Alloy for Biomedical Applications
Authors: Soni Raj , Pande Sarang , Salunkhe Sachin , Natu Harshad , Abouel Nasr Emad , Shanmugam Ragavanantham , Hussein Hussein Mohammed Abdel Moneam
Published: July 2022

Titanium (Ti) and its alloys are predominant choices for use as biomaterials in human implants. Research has shown the adverse effects of using commercial Ti alloy Ti-6Al-4V in the human body, and this presents a need for viable alternatives. In this study, Ti alloy Ti-17Nb-6Ta was manufactured by laser cladding—a prominent additive manufacturing (AM) technology. Laser cladded specimens were evaluated for their in vitro and electrochemical behavior. A human osteosarcoma cell line (MG-63 cells) was used for in vitro investigations. Cell proliferation was good in the physiological medium, and cells were alive when in contact with the laser cladded alloy, even after two to three weeks, indicating good cell viability and compatibility with this alloy. Electrochemical characterization was carried out in Ringer’s solution, and noticeably lower corrosion current density and corrosion rate values were observed. The lower amounts of these parameters indicated the passivation behavior due to multi-layer Ti, Nb, and Ta alloy oxide films. These oxide films also enhanced osseointegration. Thus, the Ti-17Nb-6Ta alloy can be an ideal biocompatible alternative to Ti-6Al-4V.
Which Are the Best Site and Stand Conditions for Silver Fir (Abies alba Mill.) Located in the Carpathian Mountains?
Authors: Dinca Lucian , Marin Mirabela , Radu Vlad , Murariu Gabriel , Drasovean Romana , Cretu Romica , Georgescu Lucian , Timiș-Gânsac Voichița
Published: July 2022

Silver fir (Abies alba Mill.) is one of the most valuable and productive tree species across European mountains, that accomplish multiple economic, protective and ecologic functions. Alongside spruce (Picea abies (L.) Karst) and beech (Fagus sylvatica L.), silver fir is a characteristic species for the Romanian Carpathians. Although silver fir tree is recommended for the diversification of forests in order to increase the resistance to climate change, it is very sensitive to climatic excesses, especially those that proceed rapidly. Therefore, the aim of this study is to investigate both the environmental conditions and stand characteristics of fir from five mountain ranges of the Romanian Carpathians. The study is based on data recorded over a period of 10 years (1990–2000). As such, a total of 77,251 stands that occupy 211,954 hectares have been investigated in regard to silver fir behaviour. MATLAB scripts were used for analysing consistent data volumes as well as the impact of eight factors on the silver fir productivity (altitude, field aspect, field slope, soil type, participation percentage, road distance, structure and consistency). Our analysis has revealed that higher silver fir productivity is found at altitudes of up to 1200 m, on mid and upper slopes, on NW field aspects, on eutric cambisols and dystric cambisols, with a 10–20% participation in stand composition and in relatively-even aged stands with a full consistency. This study offers valuable insights for forest managers that require comprehensive information in adopting effective strategies to enhance forest resilience under climate change.
eDNA Reveals the Associated Metazoan Diversity of Mediterranean Seagrass Sediments
Authors: Wesselmann Marlene , Geraldi Nathan R. , Marbà Núria , Hendriks Iris E. , Díaz-Rúa Rubén , Duarte Carlos M.
Published: July 2022

Anthropogenic impacts on marine ecosystems have led to a decline of biodiversity across the oceans, threatening invaluable ecosystem services on which we depend. Ecological temporal data to track changes in diversity are relatively rare, and the few long-term datasets that exist often only date back a few decades or less. Here, we use eDNA taken from dated sediment cores to investigate changes over approximately the last 100 years of metazoan communities in native (Cymodocea nodosa and Posidonia oceanica) and exotic (Halophila stipulacea) seagrass meadows within the eastern Mediterranean Sea, at two locations in Greece and two in Cyprus. Overall, metazoan communities showed a high turnover of taxa during the past century, where losses of individual taxa in a seagrass meadow were compensated by the arrival of new taxa, probably due to the arrival of exotic species introduced in the Mediterranean Sea from the Suez Canal or the Gibraltar Strait. Specifically, bony fishes (Class Actinopteri) and soft corals (Class Anthozoa) presented significantly higher richness in the past (before the 1980s) than in the most recent time periods (from 1980–2017) and some Cnidarian orders were solely found in the past, whereas sponges and Calanoids (Class Hexanauplia), an order of copepods, showed an increase in richness since the 1980s. Moreover, the Phyla Porifera, Nematoda and the Classes Staurozoa, Hydrozoa and Ophiuroidea were detected in P. oceanica meadows but not in C. nodosa and H. stipulacea, which led to P. oceanica meadows having twice the richness of other seagrasses. The greater richness resulted from the more complex habitat provided by P. oceanica. The combination of eDNA and sediment cores allowed us to reconstruct temporal patterns of metazoan community diversity and provides a novel approach to follow natural communities back in time in the absence of time series and baseline data. The ongoing loss of P. oceanica meadows, likely to be compounded with future warming, might lead to a major loss of biodiversity and the replacement by other seagrass species, whether native or exotic, does not compensate for the loss.
Advertisement Call Variation of Two Frog Species along an Urban–Rural Gradient in Shanghai, China
Authors: Liu Ningning , Zhong Shurong , Wang Tianhou , Li Xiuzhen , Wei Li , Zou Chunjing , Zhao Shanshan , Li Ben
Published: July 2022

Urbanization has a significant influence on amphibian communities, populations, and individuals because of habitat loss, degradation of habitat quality, and habitat fragmentation. Advertisement calls of anurans are used for communication and play an important role in reproduction; however, how anthropogenic noise and habitat change caused by urbanization affect anuran advertisement calls is less well known. In this study, we examined changes in the advertisement calls of two frog species, the ornamented pygmy frog (Microhyla fissipes) and rice frog (Fejervarya multistriata), during the breeding season along an urban–rural gradient in Shanghai, China. We used the percentage of impervious area (including buildings and roads) in each 2 km-radius landscape to represent the urbanization index of 34 study sites. We then measured five advertisement call parameters (call duration, call interval, call rate, pulse rate, and dominant frequency) of these two species (100 males of F. multistriata and 89 males of M. fissipes) in each study site from May to July in 2020 and 2021. We explored how the urbanization index and other variables (air temperature, snout–vent length and calling situation (one frog species calling, or two frog species calling together)) in each study site affected these call parameters. The results showed that the dominant frequency of M. fissipes was significantly negatively related to urbanization index, and call duration and call interval were significantly negatively related to air temperature; furthermore, call rate and pulse rate were significantly positively related to air temperature, and call duration was also significantly positively related to snout–vent length. For F. multistriata, pulse rate was significantly negatively related to urbanization index, snout–vent length, and situation (two frog species calling together), but significantly positively related to air temperature; by contrast, call interval was significantly positively related to snout–vent length. We suggested that these two frog species had different responses to urbanization, which could help us to better understand the change of anuran breeding behaviors and reproductive life-history strategies in the face of rapid urbanization.
Effectiveness of Doctoral Defense Preparation Methods
Authors: Lantsoght Eva O. L.
Published: July 2022

The doctoral defense is an important step towards obtaining the doctoral degree, and preparation is necessary. In this work, I explore the relation between the way in which a doctoral candidate prepares for the defense and two important aspects of the defense: the outcome of the defense, and the student perception during and after the defense. I carried out an international survey with an 11-point Likert scale, multiple choice, and open-ended questions on the doctoral defense and analyzed the data of the 204 completed surveys using quantitative and qualitative methods. The methods I used included the statistical tests of the correlation between, on the one hand, the preparation and, on the other hand, the defense outcome and student perception. I used an inductive thematic analysis of the open-ended survey questions to gain a deeper insight into the way candidates prepared for their defense. I found that candidates most often prepare by making their presentation, reading their thesis, and practicing for the defense. The most effective measure is the mock defense, followed by a preparatory course. The conclusion of this work is that doctoral candidates need to understand the format of their defense in order to be able to prepare properly, and that universities should explore either individual pathways to the defense or pilots using a mock defense and/or preparatory course to prepare their doctoral candidates for the defense.
Design and Analysis of the Self-Biased PLL with Adaptive Calibration for Minimum of the Charge Pump Current Mismatch
Authors: Wei Xueming , Yin Renchuan , Hou Lingli , Xu Weilin , Wei Baolin
Published: July 2022

A digital adaptive mismatch calibration (DAMC) circuit is proposed to decrease the output jitter of phase-locked loop (PLL). After amplifying the phase error with a linear time amplifier (TA), the DAMC adopts a successive approximation pulse width calibration method to reduce the mismatch current of the charge pump. The PLL prototype is fabricated in a 40nm process, the static phase error of the proposed PLL can be reduced from 358 ps to 10 ps at a 50 MHz reference clock approximately, and the RMS jitter of the PLL output is reduced from 4.91 ps to 3.59 ps, and the extended DAMC area only occupies 1.3% of the whole PLL area.
Semantic Depth Data Transmission Reduction Techniques Based on Interpolated 3D Plane Reconstruction for Light-Weighted LiDAR Signal Processing Platform
Authors: Chong Taewon , Lee Dongkyu , Park Daejin
Published: July 2022

In vehicles for autonomous driving, light detection and ranging (LiDAR) is one of the most used sensors, along with cameras. LiDAR sensors that produce a large amount of data in one scan make it difficult to transmit and calculate data in real-time in the vehicle’s embedded system. In this paper, we propose a platform based on semantic depth data-based data reduction and reconstruction algorithms that reduce the amount of data transmission and minimize the errors between original and restored data in a vehicle system using four LiDAR sensors. The proposed platform consists of four LiDAR sensors, an integrated processing unit (IPU) that reduces the data of the LiDAR sensors, and the main processor that reconstructs the reduced data and processes the image. In the proposed platform, the 58,000 bytes of data constituting one frame detected by the VL-AS16 LiDAR sensor were reduced by an average of 87.4% to 7295 bytes by the data reduction algorithm. In the IPU placed near the LiDAR sensor, the memory usage increased by the data reduction algorithm, but the data transmission time decreased by an average of 40.3%. The transmission time where the vehicle’s processor received one frame of data decreased from an average of 1.79 to 0.28 ms. Compared with the original LiDAR sensor data, the reconstructed data showed an average error of 4.39% in the region of interest (ROI). The proposed platform increased the time required for image processing in the vehicle’s main processor by an average of 6.73% but reduced the amount of data by 87.4% with a decrease in data accuracy of 4.39%.
Light-Weight Classification of Human Actions in Video with Skeleton-Based Features
Authors: Kasprzak Włodzimierz , Jankowski Bartłomiej
Published: July 2022

An approach to human action classification in videos is presented, based on knowledge-aware initial features extracted from human skeleton data and on further processing by convolutional networks. The proposed smart tracking of skeleton joints, approximation of missing joints and normalization of skeleton data are important steps of feature extraction. Three neural network models—based on LSTM, Transformer and CNN—are developed and experimentally verified. The models are trained and tested on the well-known NTU-RGB+D (Shahroudy et al., 2016) dataset in the cross-view mode. The obtained results show a competitive performance with other SOTA methods and verify the efficiency of proposed feature engineering. The network has a five times lower number of trainable parameters than other proposed methods to reach nearly similar performance and twenty times lower number than the currently best performing solutions. Thanks to the lightness of the classifier, the solution only requires relatively small computational resources.
Energy-Efficient AnMBRs Technology for Treatment of Wastewaters: A Review
Authors: Tomczak Wirginia , Gryta Marek
Published: July 2022

In recent years, anaerobic membrane bioreactors (AnMBRs) technology, a combination of a biological reactor and a selective membrane process, has received increasing attention from both industrialists and researchers. Undoubtedly, this is due to the fact that AnMBRs demonstrate several unique advantages. Firstly, this paper addresses fundamentals of the AnMBRs technology and subsequently provides an overview of the current state-of-the art in the municipal and domestic wastewaters treatment by AnMBRs. Since the operating conditions play a key role in further AnMBRs development, the impact of temperature and hydraulic retention time (HRT) on the AnMBRs performance in terms of organic matters removal is presented in detail. Although membrane technologies for wastewaters treatment are known as costly in operation, it was clearly demonstrated that the energy demand of AnMBRs may be lower than that of typical wastewater treatment plants (WWTPs). Moreover, it was indicated that AnMBRs have the potential to be a net energy producer. Consequently, this work builds on a growing body of evidence linking wastewaters treatment with the energy-efficient AnMBRs technology. Finally, the challenges and perspectives related to the full-scale implementation of AnMBRs are highlighted.
Guidelines on Composite Flexible Risers: Monitoring Techniques and Design Approaches
Authors: Amaechi Chiemela , Reda Ahmed , Ja’e Idris , Wang Chunguang , An Chen
Published: July 2022

The increasing energy demand has led to more explorations in the oil and gas industry. To achieve this, marine risers and pipelines are used to convey fluid and other resources to meet the increasing demand. In recent years, hybrid flexible composite risers have become more adaptable. Flexible risers have already proven to be a popular riser solution for various floating production systems in shallow to deepwater in many parts of the world due to their good dynamic behaviour and dependability. The hybrid flexible composite riser is made up of numerous layers of plastics, flexible pipes, composites, and steel. Some innovative monitoring methods, such as Fiber Optics Bragg Gratings (FBG), are based on a clamped composite structure with embedded optical fibre. This study presents characteristics of the monitoring techniques of composite flexible riser technology. The advantages of the monitoring techniques include aiding composite riser measurements, recording data from riser deformation, improving integrity assurance, and dependability of design from stable readings. It also proposes some design approaches as guidelines that are advised, with some policy implications.
Photovoltaic/Thermal Module Integrated with Nano-Enhanced Phase Change Material: A Numerical Analysis
Authors: Cui Yuanlong , Zhu Jie , Zoras Stamatis , Hassan Khalid , Tong Hui
Published: July 2022

Solar photovoltaic-thermal (PV/T) technology is the main strategy for harvesting solar energy due to its non-polluting, stability, good visibility and security features. The aim of the project is to develop a mathematical model of a PV/T module integrated with optical filtration and MXene-enhanced PCM. In this system, a single MXene-enhanced PCM layer is attached between the PV panel and absorber pipe with solid MXene-PCM for storage and cooling purposes. Additionally, the thermal fluid is utilized in the copper absorber pipe and connected to the heat pump system for enhancing system thermal and electrical efficiency. Furthermore, the influences of the optical filtration channel height, concentration of the nanoparticles on PV surface temperature and overall system efficiency are also discussed. This study demonstrates that the annual thermal and electrical energy output can reach 5370 kWh per annum with 74.92% of thermal efficiency and 5620 kWh with 14.65% of electrical efficiency, respectively, compared to the traditional PV/T module. Meanwhile, when the optical filtration channel height and volume concentration are enhanced, they exert a negative influence on the PV surface temperature, but the overall thermal efficiency is enhanced due to low thermal resistance to heat losses and low radiation-shielding layers.
Power System Planning and Quality Control
Authors: Senjyu Tomonobu , Khosravy Mahdi
Published: July 2022

The optimum planning of the electrical power expansion and, accordingly, controlling the power quality are recent critical issues in power management [...]
Full-Order Terminal Sliding-Mode Control for Soft Open Point
Authors: Zhou Minghao , Su Hongyu , Zhou Haoyu , Wang Likun , Liu Yi , Yu Haofan
Published: July 2022

In this paper, a full-order terminal sliding-mode control method is proposed for the rectifier side and the inverter side of the soft open point (SOP). The rectifier-side DC voltage control system consists of the voltage- and current-loops with controllers which are designed using full-order sliding-mode (FOSM) to enhance the dynamic performances and anti-disturbance. The integral type virtual control signal without chattering is designed to compensate for the unmatched uncertainties including external disturbances and some parameter perturbations. The full-order terminal sliding-mode (FOTSM) controller for the current-loop can force the current response to track its reference in finite time. The inverter side power control system is designed to regulate the power. The FOTSM controller for the power-loop ensures the power-tracking accuracy under a disturbed condition. Finally, the simulations demonstrate the effectiveness of the proposed controllers for the rectifier and inverter sides in the soft open point (SOP).
Synergy Degree Evaluation of Stakeholder Engagement in Integrated Municipal Solid Waste Management: A Case Study in Harbin, China
Authors: Wang Wenna , Chu Zhujie , Zhang Tianyue
Published: July 2022

Municipal solid waste (MSW) has caused the increasing concern for environmental issues in recent years, and the wide engagement from all stakeholders of society has been involved in promoting integrated MSW management. Therefore, this study aims to identify the problems of dis-synergy among multi-stakeholders engaged in the integrated MSW management evolution, then contribute strategies to coordinated development of integrated MSW management system by bettering the engagement and interaction of different stakeholders combined with the region characteristics. From the perspective of the stakeholder theory and synergy theory, we constructed an integrated MSW management system with four stakeholder subsystems: governments, enterprises, residents, and NGO subsystems. We used integrated MSW management in Harbin as a case study and used the synergy degree model to estimate the system synergy degree from 2010 to 2019. Then, the synergetic development trend of integrated MSW management was studied, providing a feasible approach to boost the coordinated development of integrated MSW management in Harbin. The results were in concordance with the factual situation and pointed to integrated MSW management in Harbin and, although there is movement towards a more harmonious and orderly state over time, the government subsystem needs to be further reinforced.
Unearthing the Dynamics of Indonesia’s Geothermal Energy Development
Authors: Yudha Satya , Tjahjono Benny , Longhurst Philip
Published: July 2022

Indonesia has one of the world’s biggest geothermal energy reserves, accounting for 28.61 Gigawatts of electric energy (GWe). However, as of 2022, the installed geothermal capacity in Indonesia was only around 2.175 GWe, just 7.6% of its estimated potential. Geothermal energy development is required for Indonesia to empower sustainable energy systems and achieve its target of reaching 7.2 GW of geothermal energy by 2025. The geothermal energy sector is viewed as a complex dynamic system, with complicated challenges, including technical, financial, infrastructure, and many other issues. The purpose of this paper is to understand the complex nature of geothermal systems in Indonesia. To that end, this paper examines the geothermal development from a systematic and holistic standpoint, employing the interview technique to enable the conceptualization of the geothermal systems using the system dynamics (SD) approach. The SD model exhibits several underlying and important factors influencing the development of geothermal energy in Indonesia, such as capital investment, the collection of upstream data to reduce risk, infrastructure construction, pricing, incentives, permit procedures, environmental concerns, and public acceptance.
Nursery Roosts Used by Barbastelle Bats, Barbastella barbastellus (Schreber, 1774) (Chiroptera: Vespertilionidae) in European Lowland Mixed Forest Transformed by Spruce Bark Beetle, Ips typographus (Linnaeus, 1758) (Coleoptera: Curculionidae)
Authors: Rachwald Alek , Apoznański Grzegorz , Thor Katarzyna , Więcek Mirosław , Zapart Aneta
Published: July 2022

Białowieża Primeval Forest (BPF, approx. 1700 km2) is an important forest area in Europe from the point of view of the protection of natural diversity. BPF is covered with old mixed tree stands of mostly natural origin. Norway spruce is a tree species in BPF and covers approx. 27% of its area. Between 2012 and 2017 a large outbreak of the bark beetle Ips typographus (Linnaeus, 1758) took place in the forest, which transformed the stands and left many dead standing trees. At that time salvage logging had begun but was stopped due to protests by scientists and activists and for legal reasons. As a result of research conducted using a radiotelemetry method in 2020, we found that the Western barbastelle bat Barbastella barbastellus (Schreber, 1774) chooses nursery roosts in dead Norway spruce trees, showing ecological plasticity by colonizing a newly available resource. Based on this, we found that the Western barbastelle has a preference for a type of roost rather than a tree species. Insect outbreaks in forests of primary, natural, or semi-natural origin are one of the natural factors that shape the habitat. Removal of dead standing trees disrupts these processes, and in this particular case results in the disappearance of a newly appeared ecological niche.
Future Projection of CO2 Absorption and N2O Emissions of the South Korean Forests under Climate Change Scenarios: Toward Net-Zero CO2 Emissions by 2050 and Beyond
Authors: Kim Hyung-Sub , Noulèkoun Florent , Noh Nam-Jin , Son Yo-Whan
Published: July 2022

Forests mitigate climate change by absorbing CO2. However, N2O emissions in forests, which has 298 times larger global warming potential than CO2, can diminish the climate mitigation role of forests. Thus, it is crucial to project not only CO2 absorption but also N2O emissions in forests to provide a scientific basis for the 1.5 °C Paris Agreement goal. This study used a biogeochemical model, called FBD-CAN, to project CO2 absorption and N2O emissions of South Korean forests from 2021 to 2080 under three climate scenarios, including the current climate, Representative Concentration Pathway (RCP) 4.5, and RCP 8.5. From 2021 to 2080, CO2 absorption decreased from 5.0 to 1.4 Mg CO2 ha—1 year—1 under the current climate with the aging of forests, while N2O emissions increased from 0.25 to 0.33 Mg CO2 eq. ha—1 year—1. Climate change accelerated the decreasing trend in CO2 absorption and the increasing trend in N2O emissions. The subalpine region had a faster decreasing trend in CO2 absorption than the central and southern regions due to its older stand age. These findings provide scientific references for future greenhouse gas reduction plans and broaden our knowledge of the impacts of climate change on the climate mitigation role of forests.
Quality Preservation of Ready-to-Eat Prickly Pears by Peels Recycling
Authors: Panza Olimpia , Lacivita Valentina , Conte Amalia , Del Nobile Matteo Alessandro
Published: July 2022

In the current study, prickly pear peel was advantageously recycled to preserve fruit quality. Specifically, the investigated by-products were transformed into powder and then loaded into an alginate-based solution to be applied as coating to peeled prickly pears, to give an example of sustainable minimally processed fruit. For comparison, uncoated fruit, and coated prickly pears without any powder were also prepared. During storage at refrigerated temperature, coated and uncoated samples were tested for weight loss, microbial and fungal proliferation, as well as for sensory quality acceptance. The results were interesting because great differences were found between coated and uncoated fruit, in that coating the fruit delayed weight loss and spoilage, compared to uncoated fruit. Between the simple coating and the coating with peel powder, slight differences were recorded in favor of the peel-enriched coating. In fact, it allowed the promotion of better fruit preservation, and sensory quality. Therefore, prickly pear peels, that represent abundant by-products during prickly pear processing, could be advantageously recycled to preserve fruit quality.
Application of Pulsed Electric Fields to Pilot and Industrial Scale Virgin Olive Oil Extraction: Impact on Organoleptic and Functional Quality
Authors: Navarro Alberto , Ruiz-Méndez María-Victoria , Sanz Carlos , Martínez Melchor , Rego Duarte , Pérez Ana G.
Published: July 2022

The quality of virgin olive oil (VOO) is largely determined by the technology used in the industrial process of extracting the oil. Technological innovations within this field aim to strike a proper balance between oil yield and the optimal chemical composition of VOO. The application of pulsed electric fields (PEF) that cause the electroporation of the plant cell membranes favors a more efficient breakage of the olive fruit tissue, which in turn could facilitate the extraction of the oil and some of its key minor components. Pilot-scale and industrial extraction tests have been conducted to assess the effect of PEF technology on the oil extraction yield and on the organoleptic and functional quality of VOO. The best results were obtained by combining the PEF treatment (2 kV/cm) with short malaxation times and a low processing temperature. Under these conditions, PEF technology could decisively improve the oil yield by up to 25% under optimal conditions and enhance the incorporation of phenolic and volatile compounds into the oils. The PEF treatment neither affected the physicochemical parameters used to determine the commercial categories of olive oils, nor the tocopherol content. Similarly, the sensory evaluation of the PEF-extracted oils by means of a panel test did not detect the appearance of any defect or off-flavor. In addition, the intensity of positive attributes (fruity, bitter and pungent) was generally higher in PEF oils than in control oils.
Inhibition of Colon Cancer Recurrence via Exogenous TRAIL Delivery Using Gel-like Coacervate Microdroplets
Authors: Kim Sungjun , Jwa Yerim , Hong Jiyeon , Kim Kyobum
Published: July 2022

Colon cancer (CC) belongs to the three major malignancies with a high recurrence rate. Therefore, a novel drug delivery system that can prevent CC recurrence while minimizing side effects is needed. Tumor-necrosis-factor-related apoptosis-inducing ligand (TRAIL) has recently been spotlighted as a protein drug that can induce apoptosis of cancer cells specifically. However, its short in vivo half-life is still a challenge to overcome. Hence, in this study, a gel-like mPEGylated coacervate (mPEG-Coa) delivery platform was developed through electrostatic interaction of mPEG-poly(ethylene arginylaspartate diglyceride) (mPEG-PEAD) and heparin for effective protection of cargo TRAIL, subsequently preserving its bioactivity. mPEG-Coa could protect cargo TRAIL against protease. Sustained release was observed for a long-term (14 days). In addition, recurrence of HCT-116 cells was suppressed when cells were treated with TRAIL-loaded mPEG-Coa for 7 days through long-term continuous supply of active TRAIL, whereas re-proliferation occurred in the bolus TRAIL-treated group. Taken together, these results suggest that our gel-like mPEG-Coa could be utilized as a functional delivery platform to suppress CC recurrence by exogenously supplying TRAIL for a long time with a single administration.
Different Response Behavior to Therapeutic Approaches in Homozygotic Wilson’s Disease Twins with Clinical Phenotypic Variability: Case Report and Literature Review
Authors: Samadzadeh Sara , Kruschel Theodor , Novak Max , Kallenbach Michael , Hefter Harald
Published: July 2022

Background: Wilson’s disease (WD) is an autosomal-recessive disorder of copper deposition caused by pathogenic variants in the copper-transporting ATP7B gene. There is not a clear correlation between genotype and phenotype in WD regarding symptom manifestations. This is supported by the presentation of genetically identical WD twins with phenotypic discordance and different response behavior to WD-specific therapy. Case Presentation: One of the female homozygous twins (age: 26 yrs) developed writing, speaking, swallowing and walking deficits which led to in-patient examination without conclusive results but recommended genetic testing. Both sisters were tested and were heterozygous for the C.2304dupC;p(Met769Hisf*26) and the C.3207C>A;p(His1069Gln) mutation. Self-medication of the affected sibling with 450 mg D-penicillamine (DPA) did not prevent further deterioration. She developed a juvenile parkinsonian syndrome and became wheelchair-bound and anarthric. A percutaneous endoscopic gastrostomy was applied. Her asymptomatic sister helped her with her daily life. Despite the immediate increase of the DPA dose (up to 1800 mg within 3 weeks) in the severely affected patient and the initiation of DPA therapy (up to 600 mg within 2 weeks) in the asymptomatic patient after the first visit in our institution, liver function tests further deteriorated in both patients. After 2 months, the parkinsonian patient started to improve and walk again, but experienced several falls, broke her right shoulder and underwent two necessary surgical interventions. With further consequent copper elimination therapy, liver dysfunction improved in both patients, without need for orthotopic liver transplantation (LTX) in the severely affected patient. Her excellent recovery of liver and brain dysfunction was only transiently interrupted by the development of a nephrotic syndrome which disappeared after switching to Cuprior®. Unfortunately, she died from fulminant pneumonia. Conclusion: Despite identical genetic disposition, WD symptom presentations may develop differently in monozygotic twins, and they may need to be placed on a very different therapeutical regimen. The underlying gene-environment interaction is unclear so far.
Ophthalmic and Genetic Features of Bardet Biedl Syndrome in a German Cohort
Authors: Nasser Fadi , Kohl Susanne , Kurtenbach Anne , Kempf Melanie , Biskup Saskia , Zuleger Theresia , Haack Tobias B. , Weisschuh Nicole , Stingl Katarina , Zrenner Eberhart
Published: July 2022

The aim of this study was to characterize the ophthalmic and genetic features of Bardet Biedl (BBS) syndrome in a cohort of patients from a German specialized ophthalmic care center. Sixty-one patients, aged 5–56 years, underwent a detailed ophthalmic examination including visual acuity and color vision testing, electroretinography (ERG), visually evoked potential recording (VEP), fundus examination, and spectral domain optical coherence tomography (SD-OCT). Adaptive optics flood illumination ophthalmoscopy was performed in five patients. All patients had received diagnostic genetic testing and were selected upon the presence of apparent biallelic variants in known BBS-associated genes. All patients had retinal dystrophy with morphologic changes of the retina. Visual acuity decreased from ~0.2 (decimal) at age 5 to blindness 0 at 50 years. Visual field examination could be performed in only half of the patients and showed a concentric constriction with remaining islands of function in the periphery. ERG recordings were mostly extinguished whereas VEP recordings were reduced in about half of the patients. The cohort of patients showed 51 different likely biallelic mutations—of which 11 are novel—in 12 different BBS-associated genes. The most common associated genes were BBS10 (32.8%) and BBS1 (24.6%), and by far the most commonly observed variants were BBS10 c.271dup;p.C91Lfs*5 (21 alleles) and BBS1 c.1169T>G;p.M390R (18 alleles). The phenotype associated with the different BBS-associated genes and genotypes in our cohort is heterogeneous, with diverse features without genotype–phenotype correlation. The results confirm and expand our knowledge of this rare disease.
Peripheral Myelin Protein 22 Gene Mutations in Charcot-Marie-Tooth Disease Type 1E Patients
Authors: Jung Na Young , Kwon Hye Mi , Nam Da Eun , Tamanna Nasrin , Lee Ah Jin , Kim Sang Beom , Choi Byung-Ok , Chung Ki Wha
Published: July 2022

Duplication and deletion of the peripheral myelin protein 22 (PMP22) gene cause Charcot-Marie-Tooth disease type 1A (CMT1A) and hereditary neuropathy with liability to pressure palsies (HNPP), respectively, while point mutations or small insertions and deletions (indels) usually cause CMT type 1E (CMT1E) or HNPP. This study was performed to identify PMP22 mutations and to analyze the genotype–phenotype correlation in Korean CMT families. By the application of whole-exome sequencing (WES) and targeted gene panel sequencing (TS), we identified 14 pathogenic or likely pathogenic PMP22 mutations in 21 families out of 850 CMT families who were negative for 17p12 (PMP22) duplication. Most mutations were located in the well-conserved transmembrane domains. Of these, eight mutations were not reported in other populations. High frequencies of de novo mutations were observed, and the mutation sites of c.68C>G and c.215C>T were suggested as the mutational hotspots. Affected individuals showed an early onset-severe phenotype and late onset-mild phenotype, and more than 40% of the CMT1E patients showed hearing loss. Physical and electrophysiological symptoms of the CMT1E patients were more severely damaged than those of CMT1A while similar to CMT1B caused by MPZ mutations. Our results will be useful for the reference data of Korean CMT1E and the molecular diagnosis of CMT1 with or without hearing loss.
Multi-Cell-Type Openness-Weighted Association Studies for Trait-Associated Genomic Segments Prioritization
Authors: Song Shuang , Sun Hongyi , Liu Jun S. , Hou Lin
Published: July 2022

Openness-weighted association study (OWAS) is a method that leverages the in silico prediction of chromatin accessibility to prioritize genome-wide association studies (GWAS) signals, and can provide novel insights into the roles of non-coding variants in complex diseases. A prerequisite to apply OWAS is to choose a trait-related cell type beforehand. However, for most complex traits, the trait-relevant cell types remain elusive. In addition, many complex traits involve multiple related cell types. To address these issues, we develop OWAS-joint, an efficient framework that aggregates predicted chromatin accessibility across multiple cell types, to prioritize disease-associated genomic segments. In simulation studies, we demonstrate that OWAS-joint achieves a greater statistical power compared to OWAS. Moreover, the heritability explained by OWAS-joint segments is higher than or comparable to OWAS segments. OWAS-joint segments also have high replication rates in independent replication cohorts. Applying the method to six complex human traits, we demonstrate the advantages of OWAS-joint over a single-cell-type OWAS approach. We highlight that OWAS-joint enhances the biological interpretation of disease mechanisms, especially for non-coding regions.