bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic Contributions of Genes on Sex Chromosomes and Mitochondrial DNA in a Pedigreed Population
Authors: Ogawa Shinichiro , Satoh Masahiro
Published: February 2022

The genetic contribution with respect to autosomal genes has been widely used to evaluate the genetic diversity of a target population. Here, we developed a method to calculate the genetic contribution with respect to genes on sex chromosomes and mitochondrial DNA through pedigree analysis. To demonstrate the performance, we applied the methods for calculating genetic contributions to example pedigree data. To verify the results of genetic contribution calculations, we performed gene-dropping simulations mimicking flows of genes on autosomes, X and Y chromosomes, and mitochondrial DNA, and then compared the results from the simulation with the corresponding genetic contributions. To investigate the effect of pedigree error, we compared the results of genetic contribution calculations using pedigree data with and without errors. The results of gene-dropping simulation showed good agreement with the results of the genetic contribution calculation. The effect of pedigree errors on the calculation of genetic contribution depended on the error rate. Since the patterns of the genetic contributions of such genes might be different from those on autosomes, the novel approach could provide new information on the genetic composition of populations. The results are expected to contribute to the development of methods for sustainable breeding and population management.
2-[18F]FDG PET in the Management of Radioiodine Refractory Differentiated Thyroid Cancer in the Era of Thyrosin-Kinases Inhibitors: A Real-Life Retrospective Study
Authors: Gay Stefano , Raffa Stefano , De’Luca di Pietralata Anna , Bauckneht Matteo , Vera Lara , Miceli Alberto , Albertelli Manuela , Morbelli Silvia , Giusti Massimo , Ferone Diego
Published: February 2022

Purpose To evaluate the role of 2-[18F]FDGPET/CT in the follow-up of radioiodine refractory thyroid cancer (RR-TC). Methods Forty-six 2-[18F]FDGPET/CT scans from 14 RR-TC patients were considered. Thyroid function tests: thyroglobulin (Tg), levothyroxine (LT4), and tyrosine-kinases inhibitors (TKIs) assumptions were recorded. Metabolic tumour volume (MTV) and total lesion glycolysis (TLG) were calculated from each scan and correlated with clinical parameters and the overall survival (OS). Results Baseline TLG and MTV predicted OS (p = 0.027 and p = 0.035), and negative correlation with OS was also confirmed when the same parameters were measured in follow-up scans (p = 0.015 and p = 0.021). Tg also correlated with the OS; (p = 0.014; p = 0.019 and p = 0.009). However, TLG and MTV were not significantly correlated with Tg levels. MTV and TLG variation in time were reduced during TKI therapy (p = 0.045 and p = 0.013). Conclusions 2-[18F]FDGPET/CT confirmed its prognostic role at the first assessment and during the follow-up of RR-TC patients. 2-[18F]FDGPET/CT parameters seem at least partially independent from Tg. TKI therapy resulted in a measurable effect on the variation of 2-[18F]FDGPET/CT parameters over time.
Rapid Identification of Patients Eligible for Direct Emergent Computed Tomography Angiography during Acute Ischemic Stroke: The DARE-PACE Assessment
Authors: Yiang Giou-Teng , Chen Yun-Hao , Chen Pei-Ya , Hsiao Cheng-Lun , Lin Shinn-Kuang
Published: February 2022

Background: We investigated the clinical signs to establish a method for rapid identification of patients with the National Institute of Health Stroke Scale (NIHSS) score ≥ 8 eligible for direct brain CTA study; Methods: We retrospectively enrolled 2895 in patients with acute ischemic stroke (AIS). Four items in the NIHSS were selected as the main clinical signs of stroke; Results: A total of 922 (31.8%) patients had an initial NIHSS score of ≥8. The average door-to-CT time and door-to-CTA time were 13.4 ± 1.8 and 75.5 ± 44.5 min, respectively. Among 658 patients who had the priority signs, namely dense hemiplegia (D), aphasia with right arm drop (AR), and eyeball forced deviation (E), 634 patients (96.4%) with an NIHSS score ≥ 8 were identified. By using a classification and regression tree analysis, 153 patients with an NIHSS ≥ 8 were identified among 175 patients (87.4%) who had the secondary signs, namely hemiparesis with limb falls (P), aphasia (A), drowsy or worse consciousness (C), and eyeball limitation (E). The sensitivity, specificity, and accuracy were 85.4%, 97.7%, and 95.3%, respectively. Conclusions: The DARE-PACE assessment involving a checkbox list provides excellent accuracy for rapid identification of AIS patients with an NIHSS score ≥ 8 for direct CTA study to reduce the time delay for endovascular thrombectomy.
Role of EBUS-TBNA in Non-Neoplastic Mediastinal Lymphadenopathy: Review of Literature
Authors: Scano Valentina , Fois Alessandro Giuseppe , Manca Andrea , Balata Francesca , Zinellu Angelo , Chessa Carla , Pirina Pietro , Paliogiannis Panos
Published: February 2022

Mediastinal lymphadenopathy is a condition in which one or more mediastinal lymph nodes are enlarged for malignant or benign causes, generally more than 10 mm. For a long time, the only way to approach the mediastinum was surgery, while in last decades endoscopic techniques gained their role in neoplastic diseases. At the present time, EBUS is the technique of choice for studying the mediastinum in the suspicion of cancer, while there are not strong indications in guidelines for the study of benign mediastinal lymphadenopathy. We reviewed the literature, looking for evidence of the role of EBUS in the diagnostics of non-neoplastic mediastinal lymphadenopathy, with special regard for granulomatous disease, both infectious and non-infectious. EBUS is a reliable alternative to surgery in non-neoplastic mediastinal lymphadenopathy, even if more evidence is needed for granulomatous diseases other than tuberculosis and sarcoidosis.
Novel Methods for Predicting Fluid Responsiveness in Critically Ill Patients—A Narrative Review
Authors: Horejsek Jan , Kunstyr Jan , Michalek Pavel , Porizka Michal
Published: February 2022

In patients with acute circulatory failure, fluid administration represents a first-line therapeutic intervention for improving cardiac output. However, only approximately 50% of patients respond to fluid infusion with a significant increase in cardiac output, defined as fluid responsiveness. Additionally, excessive volume expansion and associated hyperhydration have been shown to increase morbidity and mortality in critically ill patients. Thus, except for cases of obvious hypovolaemia, fluid responsiveness should be routinely tested prior to fluid administration. Static markers of cardiac preload, such as central venous pressure or pulmonary artery wedge pressure, have been shown to be poor predictors of fluid responsiveness despite their widespread use to guide fluid therapy. Dynamic tests including parameters of aortic blood flow or respiratory variability of inferior vena cava diameter provide much higher diagnostic accuracy. Nevertheless, they are also burdened with several significant limitations, reducing the reliability, or even precluding their use in many clinical scenarios. This non-systematic narrative review aims to provide an update on the novel, less employed dynamic tests of fluid responsiveness evaluation in critically ill patients.
Tooth-Derived Granules in Combination with Platelet-Rich Fibrin (“Sticky Tooth”) in Socket Preservation: A Histological Evaluation
Authors: van Orten Andreas , Goetz Werner , Bilhan Hakan
Published: February 2022

Background: The maintenance of ridge volume following tooth extraction has gained more importance in the last few years. This clinical study aimed to assess the impact of autologous dentin particles mixed with injectable platelet-rich fibrin (i-PRF) on a sticky tooth mixture for socket preservation in terms of consecutive need for horizontal guided bone regeneration and histological findings. Methods: Eight extraction sockets in seven patients were included in this study. Autologous dentin particles were mixed with PRF, filled in the sockets, and covered with a cross-linked collagen membrane exposed to the oral cavity and fixated by crisscross sutures. An orthopantomogram was taken before the first surgical procedure and a CBCT prior to static computer-aided implant surgery. At the time of implant placement, cores were harvested with the aid of a trephine for histological examinations for every preserved socket. Results: No further horizontal GBR intervention was required in any cases, and the histological findings were unremarkable. The new bone was mostly cancellous and in direct contact with the remaining dentin granules. Conclusions: Within the limits of this clinical study, it may be concluded that this method is valuable for socket preservation and obtaining vital and good quality bone structure. The sticky tooth technique seems to be very efficient despite the more complex equipment.
‘Old’ Territorial Disparities and ‘New’ Spatial Patterns: Unraveling the Latent Nexus between Sustainable Development and Desertification Risk in Italy
Authors: Salvia Rosanna , Colantoni Andrea , Bianchini Leonardo , Egidi Gianluca , Polinesi Gloria , Salvati Luca , Quaranta Giovanni
Published: February 2022

Although sustainable development and desertification risk are hegemonic concepts in environmental economics, their intimate relationship was occasionally studied and made spatially explicit. The present study contributes to fill this knowledge gap by delineating a statistical procedure that investigates, at the municipal scale in Italy, the association between two composite indexes of sustainable development (SDI) and desertification risk (ESAI). Based on a refined knowledge of the local context, results of a geographically weighted regression delineate two distinctive territorial models reflecting the mutual interplay of sustainable development and desertification risk in Italy. The level of sustainable development was negatively associated with desertification risk in Southern Italy, a region classified as ‘affected’ based on the Italian National Action Plan (NAP) to combat desertification. These findings document a traditional ‘downward spiral’ between local development and early desertification processes, suggesting that a high desertification risk is associated with local contexts having structural conditions that lead to unsustainable development, e.g., population growth, industrial development, tourism pressure, crop intensification, agricultural mechanization, and land abandonment. In non-affected regions such as Northern and Central Italy, the level of sustainable development was positively associated with desertification risk, indicating that sustainability conditions can be unable, at least in some local contexts, to assure a significant containment of environmental degradation. Policy strategies reconnecting local development with more specific environmental conservation targets in development countries are increasingly required to adapt to (and differentiate on the base of) heterogeneous local contexts.
A Multisensor Fusion-Based Cooperative Localization Scheme in Vehicle Networks
Authors: Yin Ting , Zou Decai , Lu Xiaochun , Bi Cheng
Published: February 2022

Utilizing the measured distance and information exchanged between two different nodes to cooperatively locate in a mobile network has become a solution to replace global navigation satellite system (GNSS) positioning. However, the localization accuracy of the belief propagation-based cooperative localization scheme is substantially influenced by the number of neighbors. In this paper, we propose a cooperative localization scheme combined with a trajectory tracking algorithm. With an insufficient number of neighbors, the trajectory tracking algorithm is utilized to participate in the positioning process of agents. Concretely, we carry out sensor information fusion and utilize quantum-behaved, particle-swarm-optimized, bidirectional long short-term memory (QPSO–BiLSTM) as a trajectory tracking strategy, to precisely predict the positions of agents. It is evident from simulations and results that the proposed cooperative localization scheme performs better than the belief propagation (BP)-based cooperative localization scheme in position error.
The Macroeconomic Results of Diligent Resource Revenues Management: The Norwegian Case
Authors: Perifanis Theodosios Anastasios
Published: February 2022

Many commodity-exporting countries saw their revenues plummet and experienced fiscal deficits during the pandemic. The economic rebound will restore resource exports/revenues and a new round of debate will be initiated on revenues utilization. Countries will decide either to internalize revenues or capitalize them with investments abroad. Our autoregressive distributed lag (ARDL) models provide evidence of the benefits Norway enjoys since it has not internalized revenues. The currency rate, long-term bond yields, and GDP growth are insulated from prices volatility. Furthermore, the country can absorb currency appreciations/devaluations and long-term credit rate hikes through government expenditure. However, monetary steering is favored in the long term (absorbs yield increases), while in the short run it can allow for speculative activities by credit investors. Countries should not internalize resource revenues to avoid experiencing decreased competitiveness and economic growth and increased credit rates. However, the temptation will be high enough since deficits and support packages cost a lot. This study also includes years of low prices. Thus, our research reveals the extent and limitations of diligent revenue management from a country considered as a role model.
Distributed Reinforcement Learning for the Management of a Smart Grid Interconnecting Independent Prosumers
Authors: Barth Dominique , Cohen-Boulakia Benjamin , Ehounou Wilfried
Published: February 2022

In the context of an eco-responsible production and distribution of electrical energy at the local scale of an urban territory, we consider a smart grid as a system interconnecting different prosumers, which all retain their decision-making autonomy and defend their own interests in a comprehensive system where the rules, accepted by all, encourage virtuous behavior. In this paper, we present and analyze a model and a management method for smart grids that is shared between different kinds of independent actors, who respect their own interests, and that encourages each actor to behavior that allows, as much as possible, an energy independence of the smart grid from external energy suppliers. We consider here a game theory model, in which each actor of the smart grid is a player, and we investigate distributed machine-learning algorithms to allow decision-making, thus, leading the game to converge to stable situations, in particular to a Nash equilibrium. We propose a Linear Reward Inaction algorithm that achieves Nash equilibria most of the time, both for a single time slot and across time, allowing the smart grid to maximize its energy independence from external energy suppliers.
An Overview of Promising Alternative Fuels for Road, Rail, Air, and Inland Waterway Transport in Germany
Authors: Breuer Janos , Scholten Juri , Koj Jan , Schorn Felix , Fiebrandt Marc , Samsun Remzi , Albus Rolf , Görner Klaus , Stolten Detlef , Peters Ralf
Published: February 2022

To solve the challenge of decarbonizing the transport sector, a broad variety of alternative fuels based on different concepts, including Power-to-Gas and Power-to-Liquid, and propulsion systems, have been developed. The current research landscape is investigating either a selection of fuel options or a selection of criteria, a comprehensive overview is missing so far. This study aims to close this gap by providing a holistic analysis of existing fuel and drivetrain options, spanning production to utilization. For this purpose, a case study for Germany is performed considering different vehicle classes in road, rail, inland waterway, and air transport. The evaluated criteria on the production side include technical maturity, costs, as well as environmental impacts, whereas, on the utilization side, possible blending with existing fossil fuels and the satisfaction of the required mission ranges are evaluated. Overall, the fuels and propulsion systems, Methanol-to-Gasoline, Fischer–Tropsch diesel and kerosene, hydrogen, battery-electric propulsion, HVO, DME, and natural gas are identified as promising future options. All of these promising fuels could reach near-zero greenhouse gas emissions bounded to some mandatory preconditions. However, the current research landscape is characterized by high insecurity with regard to fuel costs, depending on the predicted range and length of value chains.
Application of Traction Supply System for Charging Electric Cars
Authors: Bartłomiejczyk Mikołaj , Jarzebowicz Leszek , Hrbáč Roman
Published: February 2022

The development of electromobility involves the development of electric cars charging infrastructure. The increase of the number of chargers poses new demands for the AC power grid, especially in regard to its capacity of delivering high peak power. As an alternative for the public AC power grid, urban electrified transportation systems (trams, trolleybuses, and metro) can be used for supplying electric cars chargers. The article discusses four options of integrating electric cars chargers with a traction power supply system. The option of connecting the charger to the traction overhead supply line has been selected due to the spatial availability of the power source and possibility to use regenerative braking energy for charging. A set of criteria has been developed for analysing the capability of the traction supply system to feed electric cars chargers. An exemplary feasibility analysis was carried out for trolleybus traction supply system in Gdynia, Poland. The impact of installing the charging station on specific traction supply parameters has been predicted using present-state recordings of electrical parameters and assumed charging station power. The study shows that every supply section of the considered trolleybus traction system has the capability of installing a fast-charging station, which provides opportunities of expanding the charging stations network in Gdynia.
Evaluation of the Transient Overvoltages of HVDC Transmission Lines Caused by Lightning Strikes
Authors: Zalhaf Amr S. , Zhao Ensheng , Han Yang , Yang Ping , Almaliki Abdulrazak H. , Aly Reda M. H.
Published: February 2022

High-voltage direct-current (HVDC) transmission systems are considered an outstanding solution due to high electrical losses emerging from long-distance transmission. However, HVDC transmission lines (TLs) are vulnerable to lightning strikes. In this work, the Yunnan-Guizhou 500 kV HVDC transmission system is used as a case study to evaluate the impact of lightning strikes on DC-TL overvoltages, as no research studies have been conducted to assess the lightning transient behavior of DC-TLs. A comprehensive investigation of the 500 kV DC-TL transient performance during lightning strikes is performed, taking into account different technical aspects that have not been studied in detail by previous researchers. Additionally, analysis of the back-flashover phenomenon has not been conducted well in previous work, and results on the effect of changing the lightning strike current peak and tower grounding resistance on shielding-failure flashover are quite limited. The distributed-parameter model is used to represent the DC-TL using the electromagnetic transients program (EMTP), considering real parameters of shielding wires and DC towers to study the lightning impact in the case of back-flashover and shielding-failure phenomena. Lightning strike is applied to the shielding wire, and the impact of increasing the peak value of lightning current is investigated on the back-flashover occurrence. Moreover, the influence of tower grounding resistance variation on the transient overvoltages across the tower body and back-flashover phenomenon is evaluated. From the simulation results, increasing the lightning current peak and grounding resistance results in higher overvoltages across the tower body, which increases the probability of back-flashover. Additionally, the shielding failure of the TL is assumed, and the variation impact of the lightning current peak and grounding resistance on shielding-failure flashover is investigated. The results show that the impact of the lightning current peak has a more significant impact than the grounding resistance in the case of shielding-failure flashover.
Energy Saving and Carbon Neutrality in the Funeral Industry
Authors: Lee Kuo-Hsien , Huang Chung-Chen , Chuang Sophia , Huang Cheng-Tsu , Tsai Wen-Hsien , Hsieh Chu-Lun
Published: February 2022

As a result of the global population growth since World War II, and the major impact of the COVID-19 pandemic on the increase in the number of deaths, carbon emissions resulting from cremations in the funeral industry have increased by more than initially expected. In order to achieve the goal of the Kyoto Protocol and the Paris Agreement, to reach net-zero carbon neutrality by 2050, in this study, we comprehensively examined the literature on the differences in burial methods in terms of carbon emissions, and undertook stepwise analysis of the solution’s sequence from 1990 to 2050 using the recurrence relations in the trend changes using 5-year intervals. By collecting the annual number of global deaths and calculating the average carbon emissions per death to be 245 kg, we analyzed and compared these data with the annual carbon dioxide amount and global population until 2050. In addition, the results for cremation and Cryomation were analyzed and compared to construct a model of comparative advantage. The results of this study show that Cryomation is more energy efficient and has a greater impact on carbon emission reduction than cremation because it does not require carbon emission elements such as embalming or coffins. Thus, Cryomation can effectively reduce damage to the environment. Taking appropriate strategies for the funeral industry to promote Cryomation can achieve the goals of environmental protection and sustainable development.
Design and Analysis of Dual-Rotor Modular-Stator Hybrid-Excited Axial-Flux Permanent Magnet Vernier Machine
Authors: Jia Lun , Lin Mingyao , Lin Keman , Le Wei , Yang Anchen
Published: February 2022

This paper proposes a new structure of the dual-rotor hybrid-excited axial-flux permanent magnet vernier machine (DR-HEAFPMVM) with the modular stator and the consequent-pole PM (CPM) rotor for low-speed, high torque density applications such as in-wheel electric vehicles. The tooth-wound non-overlapping armature windings and direct current (DC) excitation windings are, respectively, arranged in stator main-teeth and split-teeth to obtain the modulated and adjustable air-gap flux densities, resulting in high torque density and outstanding flux-weakening capability. First, the design considerations, operation principles, and air-gap flux density distributions of the proposed machine are elaborated based on the air-gap permeance function. Then, the influence of the pole ratios (PRs) and the DC excitation currents on the main electromagnetic performances of the DR-HEAFPMVM, such as the flux-weakening capability and back-electromotive force (back-EMF), on-load electromagnetic torque, loss distribution, and efficiencies, is investigated using the 3-D finite-element method (FEM). Results verify the feasibility of the flux adjustment of the DC excitation windings equipped in the split-tooth, and the design with a pole ratio of 8/1 tends to have higher torque density, higher machine efficiency, and considerable flux-weakening capability compared with the other two PRs.
Dietary Brewer Grain Meal with Multienzymes Supplementation Affects Growth Performance, Gut Health, and Antioxidative Status of Weaning Pigs
Authors: Boontiam Waewaree , Hong Jinsu , Kim Yoo-Yong
Published: February 2022

We conducted a 28-day feeding study on 80 weaning pigs [(Landrace × Large White) × Duroc] to determine the effects of dietary inclusion with brewer’s grain meal (BGM) and multienzymes on their growth, intestinal health, and antioxidative status. Piglets were grouped by sex and initial BW and assigned to 20 pens with four pigs each. Treatments were a corn–soybean meal-based diet with either 0.1% multienzyme addition (PC) or without (NC), and two BGM compositions fortified with 0.1% multienzyme: 10% (BGM10) and 20% (BGM20). The overall body weight, average daily weight gain, and weight gain:feed ratio were significantly greater in pigs fed BGM20 than those fed the NC diet (p < 0.05). Moreover, the BGM diets significantly increased the digestibility of total ash and ether extract, glucose, total protein, immunoglobulin A, total antioxidant capacity, superoxide dismutase, heart and small intestine weights, villus height: crypt depth ratio (VH/CD), and Lactobacillus spp. count compared with the NC diet (p < 0.05). The diarrheal rate, blood urea nitrogen, malondialdehyde, duodenal crypt depth, and Salmonella spp. count were reduced in pigs fed the BGM-supplemented diet than those fed the NC diet (p < 0.05). The diarrheal rate (p = 0.010), ether extract digestibility (p = 0.044), total protein (p = 0.044), and duodenal villus height and VH/CD (p = 0.003 and p = 0.002, respectively) decreased quadratically with the increase in BGM supplementation. Overall, diets containing up to 20% BGM with multienzyme addition improved the nutrient utilization and intestinal health in weaning pigs by suppressing pathogenic bacterial growth without compromising the overall growth of the pigs.
Using Age-0 Stocking to Assess Growth and Recruitment of Endangered Pallid Sturgeon Scaphirhynchus albus
Authors: Gosch Nathan J. C. , Gemeinhardt Todd R. , Hall Jerrod R. , Whiteman Kasey W. , Welker Tim L. , Bonneau Joseph L.
Published: February 2022

(1) Assessing growth and recruitment can be difficult during early life history, especially for rare species such as the pallid sturgeon Scaphirhynchus albus (federally endangered in the United States). One potential tool to address this knowledge gap is the stocking of age-0 individuals. (2) During July 2018, two stocking events of marked age-0 pallid sturgeon (53–56 or 41–42 days old) occurred in the lower Missouri River resulting in the release of 8495 individuals. Over the following three years, pallid sturgeon were sampled with baited trotlines and benthic trawls during routine monitoring. (3) During this period, we captured 77 individuals that recruited to age 1 or older. Only 9% of captured individuals yielded length-at-age values falling below the 95% prediction interval for pallid sturgeon raised entirely in an energetically favorable hatchery environment with abundant food resources. (4) These results suggest that the lower Missouri River is capable of supporting hatchery-level growth for young pallid sturgeon. This study also provided a unique opportunity to document recruitment to age-1 of age-0 pallid sturgeon stocked at relatively small sizes, which highlights the potential benefits of utilizing stocking to address endangered species knowledge gaps.
Phase Diagram of Dairy Protein Mixes Obtained by Single Droplet Drying Experiments
Authors: Yu Ming , Le Floch-Fouéré Cécile , Lee Jeehyun , Boissel Françoise , Jeantet Romain , Lanotte Luca
Published: February 2022

Dairy powders are mainly produced by droplet spray drying, an articulated process that enables the manufacture of high added-value goods with a long shelf life and well-preserved functional properties. Despite the recent advances, a full understanding of the mechanisms occurring at the droplet scale in drying towers and, consequently, of the impact of process parameters and processed fluid characteristics on the powder properties is far from being achieved. In the wake of previous studies based on a laboratory scale approach, in this work, we provided a global picture of the drying in droplets of dairy protein mixes, i.e., whey proteins and casein micelles, which represent crucial dairy powder ingredients. Using profile visualization and optical microscopy, we explored the shape evolution in droplets with a range of protein contents and compositions typical of commercial powder production. The observation favored the evaluation of the specific role of each protein on the evaporation dynamics, and led to the construction of a phase diagram predictive of the dry droplet shape starting from the characteristics of the initial protein dispersions. Our outcomes represent a further step shedding light on the paradigm linking the physics of drying at the microscale and the nutritional properties of complex dairy powders.
The Need to Consider Geochemistry When Interpreting Sr-Isotopes. Comment on Gregorčič et al. The Provenance of Slovenian Milk Using 87Sr/86Sr Isotope Ratios. Foods 2021, 10, 1729
Authors: Horacek Micha
Published: February 2022

I was very interested in the investigation of the 87Sr/86Sr ratio of Slovenian milk by Gregorcic et al. [...]
The Algal Polysaccharide Ulvan and Carotenoid Astaxanthin Both Positively Modulate Gut Microbiota in Mice
Authors: Pratap Kunal , Majzoub Marwan E. , Taki Aya C. , Hernandez Socorro Miranda , Magnusson Marie , Glasson Christopher R. K. , de Nys Rocky , Thomas Torsten , Lopata Andreas L. , Kamath Sandip D.
Published: February 2022

The intestinal microbial community (microbiota) is dynamic and variable amongst individuals and plays an essential part in gut health and homeostasis. Dietary components can modulate the structure of the gut microbiota. In recent years, substantial efforts have been made to find novel dietary components with positive effects on the gut microbial community structure. Natural algal polysaccharides and carotenoids have been reported to possess various functions of biological relevance and their impact on the gut microbiota is currently a topic of interest. This study, therefore, reports the effect of the sulfated polysaccharide ulvan and the carotenoid astaxanthin extracted and purified from the aquacultured marine green macroalgae Ulva ohnoi and freshwater green microalgae Haematococcus pluvialis, respectively, on the temporal development of the murine gut microbiota. Significant changes with the increase in the bacterial classes Bacteroidia, Bacilli, Clostridia, and Verrucomicrobia were observed after feeding the mice with ulvan and astaxanthin. Duration of the treatments had a more substantial effect on the bacterial community structure than the type of treatment. Our findings highlight the potential of ulvan and astaxanthin to mediate aspects of host-microbe symbiosis in the gut, and if incorporated into the diet, these could assist positively in improving disease conditions associated with gut health.
Specific Avenin Cross-Reactivity with G12 Antibody in a Wide Range of Current Oat Cultivars
Authors: Dvořáček Václav , Kotrbová-Kozak Anna , Kozová-Doležalová Jana , Jágr Michal , Hlásná Čepková Petra , Vítámvás Pavel , Kosová Klára
Published: February 2022

Current clinical studies confirm that the consumption of oats for people suffering from celiac disease is safe. Some studies have confirmed different levels of immunoreactive gluten epitopes of oats in different cultivars, while others explain these differences due to contamination with gluten-rich species or as random cross-reactivity ELISA of homologous oat epitopes with anti-wheat gliadin antibodies. The aim of our two-year study was therefore to map cross-reactive oat epitopes in a set of 132 oat cultivars using a G12-based ELISA kit. The results were focused on the varietal and annual level of cross-reactivity (interference) of avenin epitopes with the G12 antibody on the identification of potential cultivars with significantly different interferences and assessing the degree of risk of possible false-contamination with external gluten. Although repeated evaluations confirmed high year-to-year variability (RSD ≥ 30%) in approximately 2/3 of the cultivars, the content of interfering avenin epitopes with G12 did not exceed the considered safe limit (20 mg·kg−1) for celiacs. At the same time, not only annual but, above all, significant cultivar dependences in the interference of avenins to the G12 antibody were demonstrated. Genetic dependence was further confirmed in connection with the proven avenin polymorphism as well as immunoblotting with the identification of interfering peptides with the G12 antibody in the 25 and 30 kDa regions. It was the occurrence of two bands around 30 kDa that predominantly occurred in oat cultivars with a relatively higher content of cross-reactive avenins (12–16 mg·kg−1). Due to the fact that the contents of interfering avenins ranged in several cultivars even over 16 mg·kg−1, the choice of a suitable oat cultivar may be crucial for gluten-free food producers, as it reduces the risk of a possible false-response of the commercial ELISA kits when checking the real-gluten contamination.
Molybdate Recovery by Adsorption onto Silica Matrix and Iron Oxide Based Composites
Authors: Matusoiu Florin , Negrea Adina , Ciopec Mihaela , Duteanu Narcis , Negrea Petru , Svera Paula , Ianasi Catalin
Published: February 2022

Aggressive industrial development over the last century involved different heavy metals being used, including high quantities of molybdenum, which need to be treated before discharge in industrial waters. Molybdenum’s market price and industrial applicability make its recovery a big challenge. In the present study the possibility to recover molybdenum ions from aqueous solutions by adsorption on a composite material based on silica matrix and iron oxides—SiO2FexOy—was evaluated. Tests were performed in order to determine the influence of adsorbent material dose, initial solution pH, contact time and temperature over adsorption capacity of synthesized adsorbent material. For better understanding of the adsorption process, the obtained experimental data were modelled using Langmuir, Freundlich and Sips adsorption isotherms. Based on the obtained data, it can proved that the Sips isotherm was describing with better orderliness the studied process, obtaining a maximum adsorption capacity of 10.95 mg MoO42− for each gram of material. By modelling the studied adsorption process, it was proven that the pseudo-second order model is accurately describing the adsorption process. By fitting experimental data with Weber-Morris model, it was proven that MoO42− adsorption is a complex process, occurring in two different steps, one controlled by diffusion and the second one controlled by mass transfer. Further, studies were performed in order to determine the optimum pH value needed to obtain maximum adsorption capacity, but also to determine which are the adsorbed species. From pH and desorption studies, it was proven that molybdate adsorption is a physical process. In order to establish the adsorption mechanism, the thermodynamic parameters (ΔG0, ΔH0 and ΔS0) were determined.
Consanguinity and Congenital Heart Disease Susceptibility: Insights into Rare Genetic Variations in Saudi Arabia
Authors: Albesher Nour , Massadeh Salam , Hassan Sabah M. , Alaamery Manal
Published: February 2022

Congenital heart disease (CHD) encompasses a wide range of structural defects of the heart and, in many cases, the factors that predispose an individual to disease are not well understood, highlighting the remarkable complexity of CHD etiology. Evidence of familial aggregation of CHD has been demonstrated in different communities and for different cardiac lesions. Consanguinity, particularly among first cousins, is an added risk factor for these families, particularly in societies where it is considered a common cultural practice, as confirmed in previous studies conducted in Saudi Arabia and other countries. Through comprehensive genetic testing of affected families, we have been able to better understand the genetic basis of the various cardiac lesions and to delineate the molecular mechanisms involved in cardiac morphogenesis. In this review, we discuss the epidemiology and genetics of CHD in consanguineous populations focusing on Saudi Arabia as an extensive study model to address current advances and challenges in the clinical genetic diagnosis and prevention of CHD.
A Split NanoLuc Reporter Quantitatively Measures Circular RNA IRES Translation
Authors: Sehta Priyanka , Wilhelm Ann-Marie , Lin Shu-Jun , Urman Michelle A. , MacNeil Haley A. , Fuchs Gabriele
Published: February 2022

Internal ribosomal entry sites (IRESs) are RNA secondary structures that mediate translation independent from the m7G RNA cap. The dicistronic luciferase assay is the most frequently used method to measure IRES-mediated translation. While this assay is quantitative, it requires numerous controls and can be time-consuming. Circular RNAs generated by splinted ligation have been shown to also accurately report on IRES-mediated translation, however suffer from low yield and other challenges. More recently, cellular sequences were shown to facilitate RNA circle formation through backsplicing. Here, we used a previously published backsplicing circular RNA split GFP reporter to create a highly sensitive and quantitative split nanoluciferase (NanoLuc) reporter. We show that NanoLuc expression requires backsplicing and correct orientation of a bona fide IRES. In response to cell stress, IRES-directed NanoLuc expression remained stable or increased while a capped control reporter decreased in translation. In addition, we detected NanoLuc expression from putative cellular IRESs and the Zika virus 5′ untranslated region that is proposed to harbor IRES function. These data together show that our IRES reporter construct can be used to verify, identify and quantify the ability of sequences to mediate IRES-translation within a circular RNA.
Analysis of Genomic Alternative Splicing Patterns in Rat under Heat Stress Based on RNA-Seq Data
Authors: Huang Shangzhen , Dou Jinhuan , Li Zhongshu , Hu Lirong , Yu Ying , Wang Yachun
Published: February 2022

Heat stress is one of the most severe challenges faced in livestock production in summer. Alternative splicing as an important post-transcriptional regulation is rarely studied in heat-stressed animals. Here, we performed and analyzed RNA-sequencing assays on the liver of Sprague-Dawley rats in control (22 °C, n = 5) and heat stress (4 °C for 120 min, H120; n = 5) groups, resulting in the identification of 636 differentially expressed genes. Identification analysis of the alternative splicing events revealed that heat stress-induced alternative splicing events increased by 20.18%. Compared with other types of alternative splicing events, the alternative start increased the most (43.40%) after heat stress. Twenty-eight genes were differentially alternatively spliced (DAS) between the control and H120 groups, among which Acly, Hnrnpd and mir3064 were also differentially expressed. For DAS genes, Srebf1, Shc1, Srsf5 and Ensa were associated with insulin, while Cast, Srebf1, Tmem33, Tor1aip2, Slc39a7 and Sqstm1 were enriched in the composition of the endoplasmic reticulum. In summary, our study conducts a comprehensive profile of alternative splicing in heat-stressed rats, indicating that alternative splicing is one of the molecular mechanisms of heat stress response in mammals and providing reference data for research on heat tolerance in mammalian livestock.
Development Status and Perspectives of Crop Protection Machinery and Techniques for Vegetables
Authors: Wang Shilin , Xu Tao , Li Xue
Published: February 2022

Diseases and pests are important factors in vegetable cultivation; they not only affect the growth and appearance of vegetables but also affect the yield and quality. The disease and pest control of vegetables is dominated by chemical sprays, for now. As a result, the excessive use of pesticides has been a crucial factor of pesticides’ non-point source pollution, and it is also the main cause of excessive pesticide residues in vegetables. Therefore, the design of efficient plant protection machinery and technology has become an urgent demand in order to ensure the quality and safety of vegetables. In this review, the machinery and technologies for vegetable protection are introduced from the aspects of chemical control and physical control. In the aspect of chemical control, handheld sprayers, self-propelled or track sprayers, fixed-pipe spray systems, vertical and horizontal boom sprayers, unmanned aerial vehicles (UAVs) and vegetable seed treatment techniques are introduced. In the aspect of physical control, soil physical disinfection, pest trapping technologies and ozone sterilizers are introduced. Finally, the existing problems and perspectives of pesticide application sprayers and physical control equipment for vegetables are summarized. This paper can provide references for vegetable growers and researchers.
Metabolic Profiling of Organic Acids Reveals the Involvement of HuIPMS2 in Citramalic Acid Synthesis in Pitaya
Authors: Chen Jiaxuan , Yuan Yuanju , Xie Fangfang , Zhang Zhike , Chen Jianye , Zhang Rong , Zhao Jietang , Hu Guibing , Qin Yonghua
Published: February 2022

Pitayas are rich in organic acids, especially citramalic acid, which is significantly higher than the plants. However, the mechanism of citramalic acid biosynthesis remains to be fully elucidated. In this study, organic acid compositions and contents, as well as expression patterns of key genes related to organic acid metabolism were analyzed during fruit maturation of four different pitaya cultivars i.e., ‘Guanhuabai’ (GHB), ‘Guanhuahong’ (GHH), ‘Wucihuanglong’ (WCHL), and ‘Youcihuanglong’ (YCHL). The total organic acid contents increased first and then declined during fruit maturation. The main organic acids were citramalic acid during the early stages of GHB, GHH, and WCHL pitayas, and dominated by malic acid as fruit maturation. In comparison, citric acid and malic acid were main organic acid for ‘YCHL’ pitaya. Citramalate synthase (IPMS) was involved in the synthesis of citramalic acid, and three types of HuIPMS i.e., HuIPMS1, HuIPMS2, and HuIPMS3, were obtained in our study. Highest expression levels of HuIPMS1 were detected in sepals, while HuIPMS2 and HuIPMS3 exhibited preferential expression in tender stems and ovaries. The expression levels of HuIPMS2 and HuIPMS3 were positively correlated with the content of citramalic acid in the four pitaya cultivars. HuIPMS2 was a chloroplast-localized protein, while HuIPMS3 presented a cytoplasmic-like and nuclear subcellular localization. These findings provide an important basis for further understanding of the molecular mechanism that leads to citramalic acid metabolism during pitaya fruit maturation.
Measuring the Supply Chain Performance of the Floricultural Sector Using the SCOR Model and a Multicriteria Decision-Making Method
Authors: Rodríguez Mañay Luís Oswaldo , Guaita-Pradas Inmaculada , Marques-Perez Inmaculada
Published: February 2022

This study aims to highlight the usefulness of studying the performance of supply chains (SC) at the sectoral level in greater detail through the combination of a disaggregated supply chain operations reference (SCOR) model, with a multicriteria decision-making approach, specifically using an AHP, to adjust the analysis to the particularities of the sector under study by stakeholders’ judgements. The methodology was applied to the Ecuadorian flower industry, and the data for the analysis was from a survey of a group of companies that represent this sector. In addition, a focus group of SC experts weighted the model constructs as part of the analytic hierarchy process (AHP), and then the performance level for each construct was determined. According to the results methodologies, this model allows the classification of companies by their performance, as well as the performance of the aggregate sector. The processes that Ecuadorian flower companies need to improve on are planning, procurement, and manufacturing. The study’s main contribution is developing a general framework for measuring the overall performance of SCs and how the results are obtained. This tool could help managers, consultants, industries, and governments to assess the performance of SCs, as well as improving SC management in order to increase the sector’s competitiveness in the international market.
Editorial for Special Issue “Advances in the Ecohydrology of Arid Lands”
Authors: Micklin Philip P. , Luo Pingping
Published: February 2022

Ecohydrology is an emerging, cross disciplinary subfield of hydrology devoted to the mutual interactions between water and ecosystems [...]
Association between Pet Ownership and Mental Health and Well-Being of Canadians Assessed in a Cross-Sectional Study during the COVID-19 Pandemic
Authors: Denis-Robichaud José , Aenishaenslin Cécile , Richard Lucie , Desmarchelier Marion , Carabin Hélène
Published: February 2022

The objective of this cross-sectional study was to assess the association between pet ownership and quality of life (QoL), loneliness, anxiety, stress, overall health, and mental health of Canadians during the COVID-19 pandemic using a One Health perspective. An online bilingual survey was completed by 1500 Canadians in April–May 2021. Socio-demographics, health, QoL, stress and anxiety, loneliness, social support, pet ownership, and attitude towards pets data were collected. Crude and adjusted associations between pet ownership and mental health and well-being indicators were estimated. The 1500 participants were from all provinces and territories, half were women; half of the participants were pet owners by design. The crude association estimates showed that pet owners had poorer QoL, overall health, and mental health than non-pet owners, and were lonelier, more stressed, and more anxious than non-pet owners. Adjusted estimates showed that these associations disappeared with the inclusion of the confounders (socio-economic, demographic, health, and pet-related variables). Our results suggest that there was no association between pet ownership and the mental health and well-being indicators measured in the present study.
Influence of “Face-to-Face Contact” and “Non-Face-to-Face Contact” on the Subsequent Decline in Self-Rated Health and Mental Health Status of Young, Middle-Aged, and Older Japanese Adults: A Two-Year Prospective Study
Authors: Fujiwara Yoshinori , Nonaka Kumiko , Kuraoka Masataka , Murayama Yoh , Murayama Sachiko , Nemoto Yuta , Tanaka Motoki , Matsunaga Hiroko , Fujita Koji , Murayama Hiroshi , Kobayashi Erika
Published: February 2022

This study aims to identify the independent influence of face-to-face contact (FFC) and non-face-to-face contact (NFFC) on the subsequent decline in self-rated health and mental health status by age. A total of 12,000 participants were randomly selected among residents in the study area, and 1751 of them responded to both the 2016 and 2018 mail surveys. The participants were subsequently classified into three age groups (25–49: Young adults; 50–64: Mid-aged adults; and 65–84: Older adults). Social contact was assessed by computing the frequencies of FFC and NFFC. Multiple logistic regression analysis showed the risk of social contact on the decline in self-rated health and World Health Organization-Five Well-Being Index. Both FFC and NFFC were significantly associated with maintaining mental health; however, the impacts of FFC on mental health were more significant than that of NFFC among older adults and young adults. Compared with the no contact group, FFC was significantly associated with maintaining self-rated health in mid-aged adults. The influence of FFC and NFFC on health differed by age group.