bluuebunny/crossref_metadata_2025 huggingface dataset:
Racialized Pandemic: The Effect of Racial Attitudes on COVID-19 Conspiracy Theory Beliefs
Authors: Farhart Christina E. , Chen Philip Gordon
Published: March 2022

As national and international health agencies rushed to respond to the global spread of a novel coronavirus (SARS-CoV-2, commonly known as COVID-19), one challenge these organizations faced was the spread of misinformation and conspiracy theories about the virus. Troublingly, much of the misinformation was couched in racialized language, particularly regarding the source of the virus and responsibility for its spread, fostering the development of related conspiracy theories. Media coverage of these conspiracy theories, particularly early on in the pandemic, had negative impacts on individuals' engagement in protective behaviors and concern with the spread of COVID-19. From extant work, racial resentment and white identity have been shown to be deeply woven into the fabric of contemporary American politics, affecting perceptions of public opinion even after accounting for social and political identities. While racial attitudes have been less studied in relation to conspiracy theory belief, we expect racial resentment and white identity to affect compliance with public health behaviors and COVID-19 conspiracy theory belief. Using observational and experimental survey data (N= 1,045), quota-sampled through Lucid Theorem (LT) in the spring of 2020, we demonstrate that framing the virus in racialized language alters endorsement of COVID-19 conspiracy theories, contingent upon levels of racial resentment and white identity and find that higher levels of conspiracy theory belief decreased compliance with preventative measures.
Musculoskeletal Disorders and Quality of Life in Chilean Teachers: A Cross-Sectional Study
Authors: Vega-Fernández Gustavo , Olave Enrique , Lizana Pablo A.
Published: March 2022

Introduction

Teaching professionals have reported multiple conditions associated with low quality of life (QoL) perception. Various studies have also reported high prevalence of musculoskeletal disorders (MSD). In this context, there are few studies regarding the association between low QoL perception and MSD suffering in teachers.

Objective

Therefore, in this study the aim was evaluate MSD prevalence and its association with teachers' QoL perception.

Participants and Methods

A total sample of 544 Chilean teachers were included in a cross-sectional study. MSD prevalence was evaluated via the Standardized Nordic Questionnaire and QoL perception via the Short-Form 36 Health Survey Instrument. Multinomial logistic regression was applied to evaluate the association between MSD and QoL perception adjusted for gender and age.

Results

A total of 91% of teachers have had some MSD in the last 12 months, and 28.86% have had 6 or more painful regions. Females showed greater MSD prevalence than males. Teachers who reported no MSD had higher QoL scores compared to teachers with MSD. The group of teachers with the most MSD (≥p75) saw significant increases in the risk of having low scores in the physical (OR: 2.82) and mental components (OR: 2.65) of QoL. By contrast, teachers without MSD have a buffer effect for their QoL (physical OR: 0.2; mental OR: 0.44).

Conclusions

High MSD prevalence suggest that preventive and informative actions must be taken regarding these disorders to protect teachers' mental and physical health, considering the multiple risk factors to which teachers are exposed given their work conditions in Chile and worldwide.
Guidelines for Robot-to-Human Handshake From the Movement Nuances in Human-to-Human Handshake
Authors: Cabibihan John-John , El-Noamany Ahmed , Ahmed Abdelrahman Mohamed Ragab M. , Ang Marcelo H.
Published: March 2022

The handshake is the most acceptable gesture of greeting in many cultures throughout many centuries. To date, robotic arms are not capable of fully replicating this typical human gesture. Using multiple sensors that detect contact forces and displacements, we characterized the movements that occured during handshakes. A typical human-to-human handshake took around 3.63 s (SD = 0.45 s) to perform. It can be divided into three phases: reaching (M = 0.92 s, SD = 0.45 s), contact (M = 1.96 s, SD = 0.46 s), and return (M = 0.75 s, SD = 0.12 s). The handshake was further investigated to understand its subtle movements. Using a multiphase jerk minimization model, a smooth human-to-human handshake can be modelled with fifth or fourth degree polynomials at the reaching and return phases, and a sinusoidal function with exponential decay at the contact phase. We show that the contact phase (1.96 s) can be further divided according to the following subphases: preshake (0.06 s), main shake (1.31 s), postshake (0.06 s), and a period of no movement (0.52 s) just before both hands are retracted. We compared these to the existing handshake models that were proposed for physical human-robot interaction (pHRI). From our findings in human-to-human handshakes, we proposed guidelines for a more natural handshake movement between humanoid robots and their human partners.
Life Course Tobacco Smoking and Risk of HPV-Negative Squamous Cell Carcinomas of Oral Cavity in Two Countries
Authors: Madathil Sreenath , Rousseau Marie-Claude , Durán Doris , Alli Babatunde Y. , Joseph Lawrence , Nicolau Belinda
Published: March 2022

Background

Tobacco smoking remains one of the major risk factors for oral cavity cancers (OCC), a subgroup of head and neck cancer (HNC) less attributed to human papillomavirus (HPV) infection. Although a strong dose-dependent association between tobacco smoking and OCC exists, several important questions on the age-dependent effects of this habit remain unanswered. We investigated which life course hypothesis best describes the association between tobacco smoking and HPV-negative (HPV−ve) OCC in Canada and India.

Methods

We used data from the HeNCe Life study, a hospital-based case-control study conducted in Canada and India, using similar protocols. Cases were newly diagnosed subjects with primary squamous cell carcinomas of the head and neck region. Control subjects were patients with non-cancer selected from various outpatient clinics in a hospital located in the same catchment area as the cases and frequency-matched to cases according to age and sex. We collected information on an array of life course exposures using a structured questionnaire with the help of a life grid. Tobacco exposure (pack-years) during three life periods (≤ 30, 31−50, and >50 years of age) was calculated from the entire life course history of smoking. We used CDx brushes to collect oral exfoliated cells. Alpha HPV DNA detection and genotyping were performed for 36 HPV genotypes using the linear array. Participants who tested positive for HPV were excluded from the analysis. We used the Bayesian relevant life course exposure model (BRLM) to identify the life course hypothesis that best described the relationship between tobacco smoking and HPV−ve OCC.

Results

We show evidence for a late-life sensitive period (>50 years of age) for tobacco smoking in relation to the risk of HPV−ve OCC in both Canada and India. An increase of 1 pack-year of tobacco smoking increased the risk of OCC by ~3% in both countries.

Conclusion

Our findings from the Canadian and Indian data suggest that smoking tobacco after 50 years of age may carry a higher risk of developing oral cancer than earlier in life. Further studies are warranted to confirm the results.
Pattern-Based Augmented Reality Authoring Using Different Degrees of Immersion: A Learning Nugget Approach
Authors: Rau Linda , Döring Dagny C. , Horst Robin , Dörner Ralf
Published: March 2022

Creating Augmented Reality (AR) applications can be an arduous process. With most current authoring tools, authors must complete multiple authoring steps in a time-consuming process before they can try their AR application and get a first impression of it. Especially for laypersons, complex workflows set a high barrier to getting started with creating AR applications. This work presents a novel authoring approach for creating mobile AR applications. Our idea is to provide authors with small, ready-to-use AR applications that can be executed and tested directly as a starting point. Authors can then focus on customizing these AR applications to their needs without programming knowledge. We propose to use patterns from application domains to further facilitate the authoring process. Our idea is based on the learning nugget approach from the educational sciences, where a nugget is a small and self-contained learning unit. We transfer this approach to the field of AR authoring and introduce an AR nugget authoring tool. The authoring tool provides pattern-based self-contained AR applications, called AR nuggets. AR nuggets use simple geometric objects to give authors an impression of the AR application. By replacing these objects and further adaptions, authors can realize their AR applications. Our authoring tool draws from non-immersive desktop computers and AR devices. It synchronizes all changes to an AR nugget both to an AR device and a non-immersive device. This enables authors to use both devices, e.g., a desktop computer to type text and an AR device to place virtual objects in the 3D environment. We evaluate our proposed authoring approach and tool in a user study with 48 participants. Our users installed the AR nugget authoring tool on their own devices, worked with it for 3 weeks, and filled out a questionnaire. They were able to create AR applications and found the AR nugget approach supportive. The users mainly used the desktop computer for the authoring tasks but found the synchronization to the AR device helpful to experience the AR nuggets at any time. However, the users had difficulties with some interactions and rated the AR nugget authoring tool in a neutral field.
Characterization of the Colloidal Properties of Dissolved Organic Matter From Forest Soils
Authors: Meklesh Viktoriia , Gentile Luigi , Andersson Erika , Bhattacharya Abhishek , de Farias Marcelo A. , Cardoso Mateus B. , Stålbrand Henrik , Loh Watson , Škerlep Martin , Kritzberg Emma , Tunlid Anders , Olsson Ulf , Persson Per
Published: March 2022

Components of dissolved organic matter (DOM) span from sub-nm molecules to colloidal aggregates of several hundred nm. The colloidal fraction is important for the transport of organic matter and associated elements in the environment, and for the stability of DOM constituents with respect to microbial decomposition. This study focuses on the colloidal properties of DOM extracted from spruce forest soils of a chronosequence. The DOM samples were obtained by common water extraction procedures at 21 and 100°C, respectively. We applied an experimental approach combining chemical analysis with light and X-ray scattering techniques that informed on the colloidal size, charge, and structure of DOM. Results showed that two main types of colloids were present: semi-flexible cylinders and fractal aggregates. The cylinders consisted of carbohydrates, presumably hemicelluloses, while the aggregates were a composite material containing a large fraction of carbohydrates together with aliphatics and clay particles. These fractal aggregates dominated the cold-water extracts whereas the strong increase in total organic carbon by hot-water extraction caused a concomitantly strong increase of semi-flexible cylinders, which became the predominant species. Comparison between the chronosequence soils showed that with increasing forest age, the amount of carbon extracted per gram of soil declined and the concentration of the semi-flexible cylinders decreased. Thus, the distribution between the fractal aggregates and cylinders in the forest soil DOM samples depends on the composition of the soil organic matter and the leaching temperature. Changes in this distribution may have important implications for the reactivity and stability of DOM colloids.
Selection of Amino Acid Chirality Induced by Cyclic Dipeptide Synthesis in Plausible Prebiotic Conditions
Authors: Guo Yeting , Zhang Yumeng , Ying Jianxi , Liu Yan , Zhang Gaiyun , Zhao Yufen
Published: March 2022

The biological system uses L-amino acids (L-AAs) to construct peptides. The selection of AA chirality in the prebiotic Earth still remains a mystery. Here we report the intrinsic mechanism of AA chiral selection in the formation of prebiotic proline-containing cyclic dipeptide (DKPs). The DKPs were synthesized by equal amounts of L-AA and D-AA with L-Pro that exhibited a clear preference for D-AA as opposed to L-AA. And surprisingly, the L-AA was preferred over D-AA in the mirror-image system with D-Pro. These results indicate that prebiotic DKPs synthesis has a certain selectivity for AA chirality preceded the biological enzymatic catalysis.
Open Repair of Large Hepatic Artery Pseudoaneurysm Without Collateral Circulation: A Case Report
Authors: Wen Xin , Chen Xiyang , Zhao Jichun , Luo Xin , Guo Qiang , Du Xiaojiong , Yuan Ding , Huang Bin
Published: March 2022

Hepatic artery pseudoaneurysm is a rare arterial disease. This case report describes a patient with hepatic artery pseudoaneurysm who presented with recurrent epigastric pain over a 4-month period. Computed tomography angiography (CTA) showed aneurysmal enlargement of the hepatic artery measuring 55 mm × 46 mm. The angiographic information is as follows: (1) the common hepatic artery originated from the superior mesenteric artery; (2) the proper hepatic artery originated from the common hepatic artery; (3) the proper hepatic aneurysmal disease had no collateral circulation. After careful consideration, the patient underwent an open surgical repair (OSR). The patient recovered well without any associated complications. The 1-year follow-up of patients did not reveal any relevant complications. The treatment choice, puzzles, and reflections of this case are all discussed in this article.
Phytochemical Analysis and Characterization of Corn Silk (Zea mays, G5417)
Authors: Singh Jyoti , Inbaraj Baskaran Stephen , Kaur Sawinder , Rasane Prasad , Nanda Vikas
Published: March 2022

Corn silk has long been thought of as a waste product; however, due to its numerous therapeutic attributes, it has remarkably gained popularity in Asian and African countries. Therefore, this study aimed to assess the bioactivity of dried corn silk powder (Zea mays, G5417) in terms of its physicochemical and bio-functional characteristics. The protein (15.29 ± 1.23) and ash (5.29 ± 0.29) contents in the corn silk powder were found to be high. The high phenolic content (94.10 ± 0.26 mg GAE/g) and flavonoid content (163.93 ± 0.83 mg QE/100 g) are responsible for its high antioxidant activity. The corn silk powder showed 45.40 ± 0.92% FRSA, 75.25 ± 0.59 TEAC mg/gdw of ABTS, and 86.77 ± 0.88% of FRAP. FT-IR spectroscopy revealed stretching, bending, and vibrations of abundantly present polysaccharides and protein functional groups. Moreover, the DSC thermograph revealed the exothermic reactions at on-set temperature (Tonset) = 21.9 °C and end temperature (Tendset) = 102.80 °C, and exothermic reactions at on-set temperature (Tonset) = 252.02 °C, end temperature (Tendset) = 296.80 °C, and denaturation peak temperature (Tpeak) = 277.48 °C, whereas XRD (2θ = 21.5°) confirmed the amorphous nature of the corn silk powder. Therefore, due to the potential bioactivity and thermal stability, dry corn silk powder can be scaled up at an industrial level.
Understanding Internal and External Drivers Influencing the Prescribing Behaviour of Informal Healthcare Providers with Emphasis on Antibiotics in Rural India: A Qualitative Study
Authors: Khare Shweta , Pathak Ashish , Stålsby Lundborg Cecilia , Diwan Vishal , Atkins Salla
Published: March 2022

Globally, Antibiotic resistance is a major public health concern, with antibiotic use contributing significantly. Targeting informal healthcare providers (IHCPs) is important to achieve universal health coverage and effective antibiotic stewardship in resource-constrained settings. We, therefore, aimed to analyse the internal and external drivers that influence IHCPs’ prescribing behaviour for common illnesses in children under five, with an emphasis on antibiotic use in rural areas of India. A total of 48 IHCPs participated in focus group discussions. Thematic framework analysis with an inductive approach was used, and findings were collated in the theoretical framework based on knowledge, attitude, and practice model which depicted that the decisions made by IHCPs while prescribing antibiotics are complex and influenced by a variety of external and internal drivers. IHCPs’ internal drivers included the misconception that it is impossible to treat a patient without antibiotics and that antibiotics increase the effectiveness of other drugs and cure patients faster in order to retain them. Formal healthcare providers were the IHCPs’ sources of information, which influences their antibiotic prescribing. We found when it comes to seeking healthcare in rural areas, the factors that influence their choice include ‘rapid cure’, ‘cost of treatment’, ‘distance’ and ‘24 h availability’, instead of qualification, which may create pressure for IHCPs to provide a quick fix. Targeted and coordinated efforts at all levels will be needed to change the antibiotic prescribing practices of IHCPs with a focus on behaviour change and to help resolve misconceptions about antibiotics.
The Impact of Fermentation on Bee Pollen Polyphenolic Compounds Composition
Authors: Adaškevičiūtė Vaida , Kaškonienė Vilma , Barčauskaitė Karolina , Kaškonas Paulius , Maruška Audrius
Published: March 2022

Bee-collected pollen is one of the most valuable natural products. However, the pollen cell walls limit the digestibility and release of nutrients to the human body. Solid-state lactic acid fermentation can be used to ease the release of bioactive compounds from the pollen cell. The aim of this research was to determine the impact of a solid-state lactic acid fermentation process on biologically active compound composition and antioxidant activity of bee-collected pollen from various European regions (Italy, Netherlands, Lithuania, Poland, Sweden, Denmark, Malta, Slovakia, and Spain). Spontaneous fermentation and fermentation using an L. rhamnosus culture were performed. The total content of phenolic compounds, total content of flavonoids, and radical (DPPH) scavenging activity were measured by spectrophotometric tests, while UPLC was employed for quantification of phenolic compounds. The determined fermentation positive effects included an increase of total phenolic content by 1.4–2.3 times, total flavonoid content by 1.1–1.6 times, and radical scavenging activity by 1.4–2.3 times. Naringenin (21.09–135.03 µg/g), quercetin (6.62–78.86 µg/g), luteolin (29.41–88.90 µg/g), and rutin (21.40–89.93 µg/g) were the most abundant flavonoids in all samples; however, their variation level was both geographical in origin and fermentation-type dependent. Fermentation increased the content of phenolic acids with high antioxidant potentials such as ellagic, ferulic and caffeic, while reduction of chlorogenic acid was determined.
Resveratrol Stabilization and Loss by Sodium Caseinate, Whey and Soy Protein Isolates: Loading, Antioxidant Activity, Oxidability
Authors: Yin Xin , Cheng Hao , Wusigale  , Dong Huanhuan , Huang Weining , Liang Li
Published: March 2022

The interaction of protein carrier and polyphenol is variable due to their environmental sensitivity. In this study, the interaction between resveratrol and whey protein isolate (WPI), sodium caseinate (SC) and soy protein isolate (SPI) during storage were systematically investigated from the aspects of polyphenol loading, antioxidant activity and oxidability. It was revealed that resveratrol loaded more in the SPI core and existed both in the core of SC micelles and on the particle surface, while WPI and resveratrol mainly formed in complexes. The loading capacity of the three proteins ranked in order SC > SPI > WPI. ABTS assay showed that the antioxidant activity of the protein carriers in the initial state was SC > SPI > WPI. The results of sulfhydryl, carbonyl and amino acid analysis showed that protein oxidability was SPI > SC > WPI. WPI, with the least oxidation, improved the storage stability of resveratrol, and the impact of SC on resveratrol stability changed from a protective to a pro-degradation effect. Co-oxidation occurred between SPI and resveratrol during storage, which refers to covalent interactions. The data gathered here suggested that the transition between the antioxidant and pro-oxidative properties of the carrier is the primary factor to investigate its protective effect on the delivered polyphenol.
Exploring the Diversity of the Thioredoxin Systems in Cyanobacteria
Authors: Mallén-Ponce Manuel J. , Huertas María José , Florencio Francisco J.
Published: March 2022

Cyanobacteria evolved the ability to perform oxygenic photosynthesis using light energy to reduce CO2 from electrons extracted from water and form nutrients. These organisms also developed light-dependent redox regulation through the Trx system, formed by thioredoxins (Trxs) and thioredoxin reductases (TRs). Trxs are thiol-disulfide oxidoreductases that serve as reducing substrates for target enzymes involved in numerous processes such as photosynthetic CO2 fixation and stress responses. We focus on the evolutionary diversity of Trx systems in cyanobacteria and discuss their phylogenetic relationships. The study shows that most cyanobacteria contain at least one copy of each identified Trx, and TrxA is the only one present in all genomes analyzed. Ferredoxin thioredoxin reductase (FTR) is present in all groups except Gloeobacter and Prochlorococcus, where there is a ferredoxin flavin-thioredoxin reductase (FFTR). Our data suggest that both TRs may have coexisted in ancestral cyanobacteria together with other evolutionarily related proteins such as NTRC or DDOR, probably used against oxidative stress. Phylogenetic studies indicate that they have different evolutionary histories. As cyanobacteria diversified to occupy new habitats, some of these proteins were gradually lost in some groups. Finally, we also review the physiological relevance of redox regulation in cyanobacteria through the study of target enzymes.
Rapid Weight Loss Coupled with Sport-Specific Training Impairs Heart Rate Recovery in Greco-Roman Wrestlers
Authors: Roklicer Roberto , Rossi Carlo , Bianco Antonino , Štajer Valdemar , Maksimovic Nemanja , Manojlovic Marko , Gilic Barbara , Trivic Tatjana , Drid Patrik
Published: March 2022

Wrestling is a sport that can be classified with the use of alternating aerobic–anaerobic metabolism with moderate but high-impact energy expenditure. Heart rate recovery (HRR) is the difference between heart rate during exercise and a certain amount of time after the start of recovery. The goal of this study was to determine the difference in HRR between two phases: high-intensity sport-specific training (HISST) combined with rapid weight loss (RWL)—phase 1 (P1) and HISST only—phase 2 (P2). Ten national-level wrestlers were included in this study. All participants underwent HISST along with RWL procedures for P1. Seven days later, during P2, an identical training session was performed with no RWL included. We found a statistically significant difference in the values obtained after the first and second minutes of recovery in the second set for both cases (p = 0.034 and p = 0.037, respectively), with higher HR values recorded in P1. It can be concluded that there is undoubtedly a difference in HRR during training and RWL compared to HISST alone. Additionally, HISST along with RWL could compromise the aerobic component of recovery.
Simulation of the Wheel-Surface Interaction Dynamics for All-Terrain Vehicles
Authors: Czapla Tomasz , Pawlak Mariusz
Published: March 2022

In this paper, a new methodology for the numerical simulation of the wheel–surface interaction has been presented. The finite-element method was combined with the discrete-element method, rigid body dynamics, and the advanced wheel–surface friction model. Compared to the current state-of-the-art, this novel approach can more realistically model the application of the traction force on the contact surface between the wheel and the soil. The rotation of a non-driven wheel is caused by the movement of the axis and the contact forces. The method that has been developed is able to assess both the longitudinal and lateral forces for a wide range of attack angles of the wheel; this is essential for calculating the traction effort of skid-steered vehicles.
Quality Control Platform for the Standardization of a Regenerative Medicine Product
Authors: Zia Silvia , Roda Barbara , Zannini Chiara , Alviano Francesco , Bonsi Laura , Govoni Marco , Vivarelli Leonardo , Fazio Nicola , Dallari Dante , Reschiglian Pierluigi , Zattoni Andrea
Published: March 2022

Adipose tissue is an attractive source of stem cells due to its wide availability. They contribute to the stromal vascular fraction (SVF), which is composed of pre-adipocytes, tissue-progenitors, and pericytes, among others. Because its direct use in medical applications is increasing worldwide, new quality control systems are required. We investigated the ability of the Non-Equilibrium Earth Gravity Assisted Dynamic Fractionation (NEEGA-DF) method to analyze and separate cells based solely on their physical characteristics, resulting in a fingerprint of the biological sample. Adipose tissue was enzymatically digested, and the SVF was analyzed by NEEGA-DF. Based on the fractogram (the UV signal of eluting cells versus time of analysis) the collection time was set to sort alive cells. The collected cells (F-SVF) were analyzed for their phenotype, immunomodulation ability, and differentiation potential. The SVF profile showed reproducibility, and the alive cells were collected. The F-SVF showed intact adhesion phenotype, proliferation, and differentiation potential. The methodology allowed enrichment of the mesenchymal component with a higher expression of mesenchymal markers and depletion of debris, RBCs, and an extracellular matrix still present in the digestive product. Moreover, cells eluting in the last minutes showed higher circularity and lower area, proving the principles of enrichment of a more homogenous cell population with better characteristics. We proved the NEEGA-DF method is a “gentle” cell sorter that purifies primary cells obtained by enzymatic digestion and does not alter any stem cell function.
Review of Immune-Related Adverse Events (irAEs) in Non-Small-Cell Lung Cancer (NSCLC)—Their Incidence, Management, Multiorgan irAEs, and Rechallenge
Authors: Vaddepally Raju , Doddamani Rajiv , Sodavarapu Soujanya , Madam Narasa Raju , Katkar Rujuta , Kutadi Anupama P. , Mathew Nibu , Garje Rohan , Chandra Abhinav B.
Published: March 2022

Immune checkpoint inhibitors (ICIs) have revolutionized the treatment of advanced malignancies, including non-small cell lung cancer (NSCLC). These agents have improved clinical outcomes and have become quite an attractive alternative alone or combined with other treatments. Although ICIs are tolerated better, they also lead to unique toxicities, termed immune-related adverse events (irAEs). A reconstituted immune system may lead to dysregulation in normal immune self-tolerance and cause inflammatory side effects (irAEs). Although any organ system can be affected, immune-related adverse events most commonly involve the gastrointestinal tract, endocrine glands, skin, and liver. They can occur anytime during the treatment course and rarely even after completion. Owen and colleagues showed that approximately 30% of patients with NSCLC treated with ICIs develop irAEs. Kichenadasse et al. conducted a thorough evaluation of multiorgan irAEs, which is of particular interest because information regarding these types of irAEs is currently sparse. It is important to delineate between infectious etiologies and symptom progression during the management of irAEs. Close consultation with disease-specific subspecialties is encouraged. Corticosteroids are the mainstay of treatment of most irAEs. Early intervention with corticosteroids is crucial in the general management of immune-mediated toxicity. Grade 1–2 irAEs can be closely monitored; hypothyroidism and other endocrine irAEs may be treated with hormone supplementation without the need for corticosteroid therapy. Moderate- to high-dose steroids and other additional immunosuppressants such as tocilizumab and cyclophosphamide might be required in severe, grade 3–4 cases. Recently, increasing research on irAEs after immunotherapy rechallenge has garnered much attention. Dolladille and colleagues assessed the safety in patients with cancer who resumed therapy with the same ICIs and found that rechallenge was associated with about 25–30% of the same irAEs experienced previously (4). However, such data should be carefully considered. Further pooled analyses may be required before we conclude about ICIs’ safety in rechallenge.
Clinical Application of Droplet Digital PCR for Hepatitis Delta Virus Quantification
Authors: Olivero Antonella , Rosso Chiara , Ciancio Alessia , Abate Maria Lorena , Nicolosi Aurora , Troshina Giulia , Armandi Angelo , Ribaldone Davide Giuseppe , Saracco Giorgio Maria , Bugianesi Elisabetta , Rizzetto Mario , Caviglia Gian Paolo
Published: March 2022

Droplet digital PCR (ddPCR) is a novel developed PCR technology providing the absolute quantification of target nucleic acid molecules without the need for a standard curve and regardless PCR amplification efficiency. Our aim was to develop a ddPCR assay for Hepatitis Delta virus (HDV)-RNA viremia quantification and then evaluate its performance in relation to real-time PCR methods. Primers and probe were designed from conserved regions of HDV genome to detect all the 8 HDV genotypes; the World Health Organization (WHO)-HDV international standard was used to calculate the conversion factor transforming results from copies/mL to IU/mL. To evaluate the clinical performance of ddPCR assay, plasma specimens of HDV-infected patients were tested and results were compared with data obtained with two real-time quantitative PCR (RT-qPCR) assays (i.e., in-house assay and commercial RoboGene assay). Analyzing by linear regression a series of 10-fold dilutions of the WHO-HDV International Standard, ddPCR assay showed good linearity with a slope coefficient of 0.966 and R2 value of 0.980. The conversion factor from copies to international units was 0.97 and the quantitative linear dynamic range was from 10 to 1 × 106 IU/mL. Probit analysis estimated at 95% an LOD of 9.2 IU/mL. Data from the evaluation of HDV-RNA in routine clinical specimen of HDV patients exhibited strong agreement with results obtained by RT-qPCR showing a concordance correlation coefficient of 0.95. Overall ddPCR and RT-qPCR showed highly comparable technical performance. Moreover, ddPCR providing an absolute quantification method may allow the standardization of HDV-RNA measurement thus improving the clinical and diagnostic management of delta hepatitis.
Clinical Data on Canabinoids: Translational Research in the Treatment of Autism Spectrum Disorders
Authors: Carreira Laura D. , Matias Francisca C. , Campos Maria G.
Published: March 2022

Translational research made with Cannabis sativa L. and its biocompounds provides data for some targeted diseases, as also symptoms associated with Autism Spectrum Disorders (ASDs). The main compounds ∆9-tetrahydrocannabinol (THC) and cannabidiol (CBD), are capable of modulating the endocannabinoid system since its dysregulation interferes with the pathophysiology of ASDs there are clinical evidence for its potential use in the treatment of the disease. Conventional therapy still has limitations, as it does not always treat the central symptoms, and there are many patients who do not respond to treatment, which demands more research on new therapies. Through the analysis of published literature on this topic, it is verified that cannabinoids, in particular CBD, improves symptoms associated with common comorbidities in ASDs. Some studies also demonstrate the therapeutic potential of these compounds in the treatment of central symptoms of autism. In addition, cannabinoid therapy to ASDs is associated with low adverse effects and a reduction in concomitant medication. Although it appears to be promising, it is essential to do the translation of this data into clinical research and some of its potential and critical gaps are discussed in this review pointing to large-scale and long-term clinical trials that should include more patients and homogeneous samples.
Clinical Heterogeneity Associated with MYO7A Variants Relies on Affected Domains
Authors: Joo Sun Young , Na Gina , Kim Jung Ah , Yoo Jee Eun , Kim Da Hye , Kim Se Jin , Jang Seung Hyun , Yu Seyoung , Kim Hye-Youn , Choi Jae Young , Gee Heon Yung , Jung Jinsei
Published: March 2022

Autosomal dominant hearing loss (ADHL) manifests as an adult-onset disease or a progressive disease. MYO7A variants are associated with DFNA11, a subtype of ADHL. Here, we examined the role and genotype–phenotype correlation of MYO7A in ADHL. Enrolled families suspected of having post-lingual sensorineural hearing loss were selected for exome sequencing. Mutational alleles in MYO7A were identified according to ACMG guidelines. Segregation analysis was performed to examine whether pathogenic variants segregated with affected status of families. All identified pathogenic variants were evaluated for a phenotype–genotype correlation. MYO7A variants were detected in 4.7% of post-lingual families, and 12 of 14 families were multiplex. Five potentially pathogenic missense variants were identified. Fourteen variants causing autosomal dominant deafness were clustered in motor and MyTH4 domains of MYO7A protein. Missense variants in the motor domain caused late onset of hearing loss with ascending tendency. A severe audiological phenotype was apparent in individuals carrying tail domain variants. We report two new pathogenic variants responsible for DFNA11 in the Korean ADHL population. Dominant pathogenic variants of MYO7A occur frequently in motor and MyTH4 domains. Audiological differences among individuals correspond to specific domains which contain the variants. Therefore, appropriate rehabilitation is needed, particularly for patients with late-onset familial hearing loss.
Diagnostic and Clinical Value of Specific Autoantibodies against Kelch-like 12 Peptide and Nuclear Envelope Proteins in Patients with Primary Biliary Cholangitis
Authors: Bauer Alicja , Habior Andrzej , Gawel Damian
Published: March 2022

Primary biliary cholangitis (PBC) is a chronic autoimmune liver disease characterized by the presence of antimitochondrial and antinuclear antibodies in patients’ serum. Here, we analyzed the reactivity of autoantibodies against a novel autoantigen, kelch-like 12 (KLHL12) protein, in a cohort of 138 PBC and 90 non-PBC patients. Additionally, we compared the reactivity of KLHL12 with antinuclear envelope antibodies: anti-gp210, anti-p62, and anti-LBR. Commercially available kits and an ‘in-house’ ELISA were used in the studies. Antinuclear envelope antibodies were detected in 65% of PBC patients and the presence of these antibodies was observed more frequently in patients diagnosed with later stages (III/IV) of PBC, according to Ludwig’s classification (p < 0.05) and were found to correlate with a higher concentration of bilirubin. Overall, anti-KLHL12 antibodies were found more frequently in PBC patients than in non-PBC controls (p < 0.001). Anti-KLHL12 antibodies were detected in 36% of the tested PBC cohort, including PBC patients negative for antimitochondrial antibodies. Presence of anti-KLHL12 was also associated with a higher concentration of bilirubin and correlated with fibrosis (p < 0.05). Anti-KLHL12 antibodies were detected in 30% of PBC individuals positive for antinuclear envelope antibodies, while anti-KLHL12 and antinuclear envelope antibodies were found in 17% of all PBC cases. Concluding, our data confirm that antibodies against the KLHL12 protein are highly specific for PBC and when used in combination with other markers, may significantly increase the diagnosis of PBC.
Synthesis, Stereochemical and Photophysical Properties of Functionalized Thiahelicenes
Authors: Pelliccioli Valentina , Cardano Francesca , Renno Giacomo , Vasile Francesca , Graiff Claudia , Mazzeo Giuseppe , Fin Andrea , Longhi Giovanna , Abbate Sergio , Rosetti Alessia , Villani Claudio , Viscardi Guido , Licandro Emanuela , Cauteruccio Silvia
Published: March 2022

We report on the synthesis of a novel class of functionalized thia[6]helicenes and a thia[5]helicene, containing a benzothiophene unit and a second heteroatom embedded in the helix (i.e., nitrogen and oxygen) or a pyrene or a spirobifluorene moiety. These systems are obtained through straightforward and general procedures that involve: (i) palladium-catalyzed annulation of iodo-atropoisomers with internal alkynes and (ii) Suzuki coupling of iodo-atropoisomers with phenyl boronic acid followed by a Mallory-type reaction. Both experimental and theoretical studies on the configurational stability of some selected thia[6]helicenes confirmed their stability toward racemization at room temperature, while the pyrene-based thia[5]helicene was found to be unstable. Moreover, the configuration assignment for one representative thiahelicene was established through the comparison between experimental and theoretical circular dichroism (CD) spectra. A systematic study of the photophysical properties of both thiahelicenes and the corresponding atropoisomers has been carried out to provide a complete overview on the new molecules proposed in this work. The obtained data showed regular trends in all the thiahelicene series with spectroscopic traits in line with those previously observed for similar heterohelicenes.
Design and Characterization of a Microwave Transducer for Gas Sensing Applications
Authors: Gugliandolo Giovanni , Naishadham Krishna , Crupi Giovanni , Donato Nicola
Published: March 2022

Gas sensors have wide applications in several fields, spanning diverse areas such as environmental monitoring, healthcare, defense, and the evaluation of personal and occupational exposure to hazardous chemicals. Different typologies of gas sensors have been proposed over the years, such as optical, electrochemical, and metal oxide gas sensors. In this paper, a relatively new typology of gas sensors is explored: the microwave gas sensor. It consists of a combination of a microwave transducer with a nanostructured sensing material deposited on an interdigitated capacitor (IDC). The device is designed and fabricated on a Rogers substrate (RO4003C) using microstrip technology, and investigated as a microwave transducer over the frequency range from 1 GHz to 6 GHz by measuring the scattering (S) parameters in response to gas adsorption and desorption. The sensing material is based on a nano-powder of barium titanate oxalate with a coating of urea (BaTiO(C2O4)2/CO(NH2)2). It is deposited on the IDC surface by drop coating, thus creating a sensing film. The developed prototype has been tested toward different oxygen (O2) concentrations and exhibits a sensitivity of 28 kHz/%O2. Special attention has been devoted to the measurement process. Besides the canonical short-open-load-thru (SOLT) calibration of the measured S-parameters, a thru-reflect-line (TRL) calibration has been performed in order to get rid of the parasitic electromagnetic (EM) contributions of the board connectors and the feedlines, thus moving the measurement reference planes to the edges of the IDC.
The Role of Surface Enhanced Raman Scattering for Therapeutic Drug Monitoring of Antimicrobial Agents
Authors: Fornasaro Stefano , Cialla-May Dana , Sergo Valter , Bonifacio Alois
Published: March 2022

The rapid quantification of antimicrobial agents is important for therapeutic drug monitoring (TDM), enabling personalized dosing schemes in critically ill patients. Highly sophisticated TDM technology is becoming available, but its implementation in hospitals is still limited. Among the various proposed techniques, surface-enhanced Raman scattering (SERS) stands out as one of the more interesting due to its extremely high sensitivity, rapidity, and fingerprinting capabilities. Here, we present a comprehensive review of various SERS-based novel approaches applied for direct and indirect detection and quantification of antibiotic, antifungal, and antituberculosis drugs in different matrices, particularly focusing on the challenges for successful exploitation of this technique in the development of assays for point-of-care tests.
Advantages of Telescopic Screw in Slipped Capital Femoral Epiphysis Treatment: A Retrospective Study and Review of the Literature
Authors: Ulici Alexandru , Ionescu Adelina , Dogaru Diana , Hotoboc Olivia , Nahoi Catalin , Zamfir Cristian , Carp Madalina
Published: March 2022

Background: Slipped capital femoral epiphysis is due to proximal femur physis failure in adolescent patients. Early iatrogenic closure of proximal growth cartilage in children with significant residual growth potential causes complications such as coxa breva, coxa vara, and lower limb length inequalities. The Free-Gliding SCFE Screw System is a self-extending cannulated screw used in Slipped Capital Femoral Epiphysis (SCFE) fixation and femoral neck fractures. Materials and Method: We conducted a retrospective study on 16 patients. All patients under 11 years old were treated by telescopic cannulated screws fixation. The youngest patient was 7 years old. Results: Out of the 22 operated hips, 2 screws have failed, thus resulting in a lack of telescoping of the screw. We discovered an average lengthening of approximately 10 mm at 24 months postoperative check-up in 20 hips in which lengthening took place. According to the Notzli method, none of the patients had an alpha angle value greater than 48 degrees. Conclusion: Fixation with telescopic screw for SCFE in patients less than 11 years old, with mild to moderate slippage, allows the continuous growth and remodeling of the proximal femur, thus avoiding deformities such as coxa breva, coxa vara, FAI, AVN, limb length discrepancies and also allows good range of motion.
Allergen Tests of Fruit Sensitization Involving Children with Allergic Diseases
Authors: Chang Ling-Sai , Chang Hsin-Yu , Yang Yao-Hsu , Lee Zon-Min , Guo Mindy Ming-Huey , Huang Ying-Hsien , Kuo Ho-Chang
Published: March 2022

Fruit is a kind of plant food which is rich in nutrients and immune-regulating ingredients. A meta-analysis has demonstrated that fruits have a protective effects against asthma. On the other hand, clinical syndromes of allergic reactions to fruits manifest as an oral allergy syndrome. We aimed to investigate the patterns and associated factors of fruit allergen-specific IgE (sIgE) sensitization among patients with suspected clinical symptoms. Data were extracted from the Chang Gung Research Database. Fruit sensitization in Taiwan was evaluated using the presence of IgE antibodies against specific fruits. The overall prevalence of positive sIgE responses to fruit allergens in Taiwan, in order of decreasing importance, was pineapple, kiwi, banana, and papaya. Children aged 0–18 had a higher positive rate of allergic responses to pineapple, kiwi, banana, and papaya than adults over the age of 18. Positive specific IgE for kiwi, banana, or papaya was more frequent in younger than in older children and children with a higher total IgE of both logarithmic (log) and arithmetic values. The analysis of log IgE for pineapple positive vs. negative children determined an optimal cutoff value, log IgE 2.2, with both sensitivity (0.9) and specificity (0.5). Dermatitis was significantly more prevalent in children with positive IgE for pineapple, kiwi, banana, and papaya than negative specific IgE. The highest positive rate of sIgE against fruits was pineapple among children. Even in older children, the positive rate of pineapple allergens was high. IgE discriminates with and without sIgE for pineapple, with an optimal cutoff of 158.5 U/mL.
Continuous Simulation of the Power Flow in AC–DC Hybrid Microgrids Using Simplified Modelling
Authors: López-Santos Oswaldo , Salas-Castaño María C. , Salazar-Dantonio Diego F.
Published: March 2022

This paper reports the development of a model for continuous simulation of the power flow into AC–DC hybrid microgrids operating for different generation–consumption scenarios. The proposed application was assembled using a multiple-input multiple-output model which was built using blocks containing simplified models of photovoltaic (PV) modules, wind turbines (WT), battery arrays (energy storage units, ESU), and power loads. The average power was used as the input/output variable of the blocks, allowing flexibility for easy reconfiguration of the microgrid and its control. By defining a generation profile, PV and WT were modeled considering environmental conditions and efficiency profiles of the maximum power point tracking (MPPT) algorithms. ESUs were modeled from intrinsic characteristics of the batteries, considering a constant power charge regime and using the State of Energy (SoE) approach to compute autonomy. To define a consumption profile, DC and AC loads were modeled as a constant real power. As an innovative characteristic, unidirectional and bidirectional power conversion stages were modeled using efficiency profiles, which can be obtained from experiments applied to the real converters. The outputs of the models of generation, consumption, and storage units were integrated as inputs of the mathematical expressions computing the power balance of the buses of the microgrid. The proposed model is suitable to analyze efficiency for different configurations of the same microgrid architecture, and can be extended by integrating additional elements. The model was implemented in LabVIEW software and three examples were developed to test its correct operation.
Effects of Continuous Cropping on Bacterial Community and Diversity in Rhizosphere Soil of Industrial Hemp: A Five-Year Experiment
Authors: Guo Li , Chen Xiangwei , Li Zeyu , Wang Mingze , Che Ye , Zhang Ling , Jiang Zeyu , Jie Siyuan
Published: March 2022

Long-term continuous monoculture cultivation harms soil physicochemical and microbial communities in agricultural practices. However, little has been reported on the effect of continuous cropping of industrial hemp on bacterial community and diversity in the rhizosphere soil. Our study investigated the changes in physicochemical properties and bacterial communities of industrial hemp rhizosphere soils in different continuous cropping years. The results showed that continuous cropping would reduce soil pH and available phosphorus (AP), while electrical conductivity (EC), available nitrogen (AN), and available potassium (AK) would increase. Soil bacterial diversity and richness index decreased with continuous cropping years. At the same time, continuous cropping marked Acidobacteria, Bacteroidetes, and Gemmatimonadetes increase, and the Proteobacteria and Actinobacteria decreased. Moreover, we found that pH, AK, and AP were the critical factors associated with the changes in the abundance and structure of the bacterial community. Overall, our study first reported the effect of continuous cropping on the rhizosphere soil microflora of industrial hemp. The results can provide a theoretical basis for revealing the obstacle mechanism of continuous cropping of industrial hemp and contribute to the sustainable cultivation of industrial hemp in the future.