bluuebunny/crossref_metadata_2025 huggingface dataset:
Tempo and Metrical Analysis by Tracking Multiple Metrical Levels Using Autocorrelation
Authors: Lartillot Olivier , Grandjean Didier
Published: November 2019

We present a method for tempo estimation from audio recordings based on signal processing and peak tracking, and not depending on training on ground-truth data. First, an accentuation curve, emphasizing the temporal location and accentuation of notes, is based on a detection of bursts of energy localized in time and frequency. This enables the detection of notes in dense polyphonic texture, while ignoring spectral fluctuation produced by vibrato and tremolo. Periodicities in the accentuation curve are detected using an improved version of autocorrelation function. Hierarchical metrical structures, composed of a large set of periodicities in pairwise harmonic relationships, are tracked over time. In this way, the metrical structure can be tracked even if the rhythmical emphasis switches from one metrical level to another. This approach, compared to all the other participants to the Music Information Retrieval Evaluation eXchange (MIREX) Audio Tempo Extraction competition from 2006 to 2018, is the third best one among those that can track tempo variations. While the two best methods are based on machine learning, our method suggests a way to track tempo founded on signal processing and heuristics-based peak tracking. Moreover, the approach offers for the first time a detailed representation of the dynamic evolution of the metrical structure. The method is integrated into MIRtoolbox, a Matlab toolbox freely available.
Modelling of Acoustic Emission Signals Due to Fiber Break in a Model Composite Carbon/Epoxy: Experimental Validation and Parametric Study
Authors: Hamam Zeina , Godin Nathalie , Fusco Claudio , Monnier Thomas
Published: November 2019

The present paper focuses on experiments and numerical simulation of the acoustic emission (AE) signals due to fiber break in a model composite. AE signals are related to wave effects due to the source, the propagation medium and the sensor. For quantitative AE analysis, it is very important to understand the effect of the piezoelectric sensors and propagation on the “primitive” AE signals. In this study, we investigate the influence of sensors, thickness, and position of the fiber by finite element simulations. This parametric study can allow an enlargement of the library for supervised classification of AE signals.
Seasonal Analysis of Cloud-To-Ground Lightning Flash Activity in the Western Antarctica
Authors: Yusop Norbayah , Ahmad Mohd Riduan , Abdullah Mardina , Mohd Esa Mona Riza , Mohammad Sulaiman Ali , Suparta Wayan , Gulisano Adriana Maria , Cooray Vernon
Published: November 2019

This paper presents a seasonal analysis of cloud-to-ground (CG) lightning flash activity in the Western Antarctica using a lightning detector sensor installed at the Carlini Base station. Data obtained from the detection system between February and December 2017 were analyzed. Three common locations and areas of composite active thunderstorms (labelled storm regions A, B, and C) were detected by the sensor within a 1000 km radius from the station. Storm region A was located to the northwest (N/W) of the station and covered the Amundsen/Bellingshausen Sea (ABS), whereas storm region C was located on the southeastern (S/E) side of the station over the Weddell Sea (WS), with distances ranging from 500 to 800 km and bearings of 270° to 360° and 90° to 180, respectively. Storm region B was located around 100 km from the station with the bearings of stroke taken from 0° to 360°. A total of 2,019,923 flashes were detected, of which 43.01% were positive CG and 56.99% were negative CG flashes. The analysis revealed that more than 96% of the CG flashes (both positive CG and negative CG) were produced during the summer and fall seasons as compared with less than 4% during the winter and spring seasons. Most detected lightning strokes (>85%) were located in the central area around the station produced by storm region B and less than 15% were produced by storm region A and storm region C, located in the ocean areas over the Amundsen/Bellingshausen Sea and the Weddell Sea.
Plastrum Testudinis Extract Mitigates Thiram Toxicity in Broilers via Regulating PI3K/AKT Signaling
Authors: Qamar Hammad , Waqas Muhammad , Li Aoyun , Iqbal Mudassar , Mehmood Khalid , Li Jiakui
Published: November 2019

Tibial dyschondroplasia (TD) negatively affects broilers all over the world, in which the accretion of the growth plate (GP) develops into tibial proximal metaphysis. Plastrum testudinis extract (PTE) is renowned as a powerful antioxidant, anti-inflammatory, and bone healing agent. The current study was conducted to evaluate the efficacy of PTE for the treatment of thiram-induced TD chickens. Broilers (day old; n = 300) were raised for 3 days with normal feed. On the 4th day, three groups (n = 100 each) were sorted, namely, the control (normal diet), TD, and PTE groups (normal diet+ thiram 50 mg/kg). On the 7th day, thiram was stopped in the TD and PTE group, and the PTE group received a normal diet and PTE (30 mg/kg/day). Plastrum testudinis extract significantly restored (p < 0.05) the liver antioxidant enzymes, inflammatory cytokines, serum biochemicals, GP width, and tibia weight as compared to the TD group. The PTE administration significantly increased (p < 0.05) growth performance, vascularization, AKT (serine/threonine-protein kinase), and PI3K expressions and the number of hepatocytes and chondrocytes with intact nuclei were enhanced. In conclusion, PTE has the potential to heal TD lesions and act as an antioxidant and anti-inflammatory drug in chickens exposed to thiram via the upregulation of AKT and PI3K expressions.
Formula for Determining the Construction Workers Productivity Including Environmental Factors
Authors: Malara Jarosław , Plebankiewicz Edyta , Juszczyk Michał
Published: November 2019

The aim of this study is to build a mathematical model of the productivity of construction workers. It does so by selecting 17 factors that influence the productivity of construction workers and categorising them into five groups. For the mathematical description of the factors, fuzzy logic was used. A formula for calculating the work productivity of construction workers is proposed. The novelty of the approach proposed by the authors is rooted in the consideration of various factors that have the potential to influence the productivity of construction workers. To present the way the formula operates, a single assessment of ceiling formwork was undertaken. The verification of a model confirmed its capability of analyzing, evaluating, and predicting the productivity of construction workers with satisfying accuracy.
Deregulated NKL Homeobox Genes in B-Cell Lymphoma
Authors: Nagel Stefan , Drexler Hans G.
Published: November 2019

Recently, we have described physiological expression patterns of NKL homeobox genes in early hematopoiesis and in subsequent lymphopoiesis. We identified nine genes which constitute the so-called NKL-code. Aberrant overexpression of code-members or ectopically activated non-code NKL homeobox genes are described in T-cell leukemia and in T- and B-cell lymphoma, highlighting their oncogenic role in lymphoid malignancies. Here, we introduce the NKL-code in normal hematopoiesis and focus on deregulated NKL homeobox genes in B-cell lymphoma, including HLX, MSX1 and NKX2-2 in Hodgkin lymphoma; HLX, NKX2-1 and NKX6-3 in diffuse large B-cell lymphoma; and NKX2-3 in splenic marginal zone lymphoma. Thus, the roles of various members of the NKL homeobox gene subclass are considered in normal and pathological hematopoiesis in detail.
Hypoxic Environment and Paired Hierarchical 3D and 2D Models of Pediatric H3.3-Mutated Gliomas Recreate the Patient Tumor Complexity
Authors: Blandin Anne-Florence , Durand Aurélie , Litzler Marie , Tripp Aurélien , Guérin Éric , Ruhland Elisa , Obrecht Adeline , Keime Céline , Fuchs Quentin , Reita Damien , Lhermitte Benoit , Coca Andres , Jones Chris , Rebel Isabelle Lelong , Villa Pascal , Namer Izzie Jacques , Dontenwill Monique , Guenot Dominique , Entz-Werle Natacha
Published: November 2019

Background: Pediatric high-grade gliomas (pHGGs) are facing a very dismal prognosis and representative pre-clinical models are needed for new treatment strategies. Here, we examined the relevance of collecting functional, genomic, and metabolomics data to validate patient-derived models in a hypoxic microenvironment. Methods: From our biobank of pediatric brain tumor-derived models, we selected 11 pHGGs driven by the histone H3.3K28M mutation. We compared the features of four patient tumors to their paired cell lines and mouse xenografts using NGS (next generation sequencing), aCGH (array comparative genomic hybridization), RNA sequencing, WES (whole exome sequencing), immunocytochemistry, and HRMAS (high resolution magic angle spinning) spectroscopy. We developed a multicellular in vitro model of cell migration to mimic the brain hypoxic microenvironment. The live cell technology Incucyte© was used to assess drug responsiveness in variable oxygen conditions. Results: The concurrent 2D and 3D cultures generated from the same tumor sample exhibited divergent but complementary features, recreating the patient intra-tumor complexity. Genomic and metabolomic data described the metabolic changes during pHGG progression and supported hypoxia as an important key to preserve the tumor metabolism in vitro and cell dissemination present in patients. The neurosphere features preserved tumor development and sensitivity to treatment. Conclusion: We proposed a novel multistep work for the development and validation of patient-derived models, considering the immature and differentiated content and the tumor microenvironment of pHGGs.
Expression of Human PTEN-L in a Yeast Heterologous Model Unveils Specific N-Terminal Motifs Controlling PTEN-L Subcellular Localization and Function
Authors: Fernández-Acero Teresa , Bertalmio Eleonora , Luna Sandra , Mingo Janire , Bravo-Plaza Ignacio , Rodríguez-Escudero Isabel , Molina María , Pulido Rafael , Cid Víctor J.
Published: November 2019

The tumour suppressor PTEN is frequently downregulated, mutated or lost in several types of tumours and congenital disorders including PHTS (PTEN Hamartoma Tumour Syndrome) and ASD (Autism Spectrum Disorder). PTEN is a lipid phosphatase whose activity over the lipid messenger PIP3 counteracts the stimulation of the oncogenic phosphatidylinositol 3-kinase (PI3K) pathway. Recently, several extended versions of PTEN produced in the cell by alternative translation initiation have been described, among which, PTEN-L and PTEN-M represent the longest isoforms. We previously developed a humanized yeast model in which the expression of PI3K in Saccharomyces cerevisiae led to growth inhibition that could be suppressed by co-expression of PTEN. Here, we show that the expression of PTEN-L and PTEN-M in yeast results in robust counteracting of PI3K-dependent growth inhibition. N-terminally tagged GFP-PTEN-L was sharply localized at the yeast plasma membrane. Point mutations of a putative membrane-binding helix located at the PTEN-L extension or its deletion shifted localization to nuclear. Also, a shift from plasma membrane to nucleus was observed in mutants at basic amino acid clusters at the PIP2-binding motif, and at the Cα2 and CBR3 loops at the C2 domain. In contrast, C-terminally tagged PTEN-L-GFP displayed mitochondrial localization in yeast, which was shifted to plasma membrane by removing the first 22 PTEN-L residues. Our results suggest an important role of the N-terminal extension of alternative PTEN isoforms on their spatial and functional regulation.
Endoplasmic Reticulum Protein Disulfide Isomerase Shapes T Cell Efficacy for Adoptive Cellular Therapy of Tumors
Authors: Hurst Katie E. , Lawrence Kiley A. , Reyes Angeles Lety , Ye Zhiwei , Zhang Jie , Townsend Danyelle M. , Dolloff Nathan , Thaxton Jessica E.
Published: November 2019

Effective cancer therapies simultaneously restrict tumor cell growth and improve anti-tumor immune responses. Targeting redox-dependent protein folding enzymes within the endoplasmic reticulum (ER) is an alternative approach to activation of the unfolded protein response (UPR) and a novel therapeutic platform to induce malignant cell death. E64FC26 is a recently identified protein disulfide isomerase (PDI) inhibitor that activates the UPR, oxidative stress, and apoptosis in tumor cells, but not normal cell types. Given that targeting cellular redox homeostasis is a strategy to augment T cell tumor control, we tested the effect of E64FC26 on healthy and oncogenic T cells. In stark contrast to the pro-UPR and pro-death effects we observed in malignant T cells, we found that E64FC26 improved viability and limited the UPR in healthy T cells. E64FC26 treatment also diminished oxidative stress and decreased global PDI expression in normal T cells. Oxidative stress and cell death are limited in memory T cells and we found that PDI inhibition promoted memory traits and reshaped T cell metabolism. Using adoptive transfer of tumor antigen-specific CD8 T cells, we demonstrate that T cells activated and expanded in the presence of E64FC26 control tumor growth better than vehicle-matched controls. Our data indicate that PDI inhibitors are a new class of drug that may dually inhibit tumor cell growth and improve T cell tumor control.
Histamine-Releasing Factor, a New Therapeutic Target in Allergic Diseases
Authors: Kawakami Yu , Kasakura Kazumi , Kawakami Toshiaki
Published: November 2019

Histamine-releasing activities on human basophils have been studied as potential allergy-causing agents for four decades. An IgE-dependent histamine-releasing factor (HRF) was recently shown to interact with a subset of immunoglobulins. Peptides or recombinant proteins that block the interactions between HRF and IgE have emerged as promising anti-allergic therapeutics, as administration of them prevented or ameliorated type 2 inflammation in animal models of allergic diseases such as asthma and food allergy. Basic and clinical studies support the notion that HRF amplifies IgE-mediated activation of mast cells and basophils. We discuss how secreted HRF promotes allergic inflammation in vitro and in vivo complex disease settings.
Role of Fibronectin in Primary Open Angle Glaucoma
Authors: Faralli Jennifer A. , Filla Mark S. , Peters Donna M.
Published: November 2019

Primary open angle glaucoma (POAG) is the most common form of glaucoma and the 2nd most common cause of irreversible vision loss in the United States. Nearly 67 million people have the disease worldwide including >3 million in the United States. A major risk factor for POAG is an elevation in intraocular pressure (IOP). The increase in IOP is believed to be caused by an increase in the deposition of extracellular matrix proteins, in particular fibronectin, in a region of the eye known as the trabecular meshwork (TM). How fibronectin contributes to the increase in IOP is not well understood. The increased density of fibronectin fibrils is thought to increase IOP by altering the compliance of the trabecular meshwork. Recent studies, however, also suggest that the composition and organization of fibronectin fibrils would affect IOP by changing the cell-matrix signaling events that control the functional properties of the cells in the trabecular meshwork. In this article, we will discuss how changes in the properties of fibronectin and fibronectin fibrils could contribute to the regulation of IOP.
Identification and Characterization of Aspergillus nidulans Mutants Impaired in Asexual Development under Phosphate Stress
Authors: Otamendi Ainara , Espeso Eduardo A. , Etxebeste Oier
Published: November 2019

The transcription factor BrlA plays a central role in the production of asexual spores (conidia) in the fungus Aspergillus nidulans. BrlA levels are controlled by signal transducers known collectively as UDAs. Furthermore, it governs the expression of CDP regulators, which control most of the morphological transitions leading to the production of conidia. In response to the emergence of fungal cells in the air, the main stimulus triggering conidiation, UDA mutants such as the flbB deletant fail to induce brlA expression. Nevertheless, ΔflbB colonies conidiate profusely when they are cultured on a medium containing high H2PO4− concentrations, suggesting that the need for FlbB activity is bypassed. We used this phenotypic trait and an UV-mutagenesis procedure to isolate ΔflbB mutants unable to conidiate under these stress conditions. Transformation of mutant FLIP166 with a wild-type genomic library led to the identification of the putative transcription factor SocA as a multicopy suppressor of the FLIP (Fluffy, aconidial, In Phosphate) phenotype. Deregulation of socA altered both growth and developmental patterns. Sequencing of the FLIP166 genome enabled the identification and characterization of PmtCP282L as the recessive mutant form responsible for the FLIP phenotype. Overall, results validate this strategy for identifying genes/mutations related to the control of conidiation.
Impact of Aggressive Media on the Properties of Polymeric Coatings with Solidification Products as Fillers
Authors: Hodul Jakub , Mészárosová Lenka , Žlebek Tomáš , Drochytka Rostislav , Dufek Zdeněk
Published: November 2019

Dealing with waste materials, particularly hazardous waste, is a serious problem. Disposal areas keep growing, and the costs incurred are high. Disposing of such waste reduces negative environmental impacts and offers considerable financial savings. This paper focuses on the possibilities of incorporating pollutants found in hazardous wastes as fillers in coatings based on polymers (epoxide and polyurethane). These coatings are intended mainly for concrete and metal bases and offer secondary protection against adverse weather conditions. Important physical and mechanical properties of the newly developed materials were determined; they include surface hardness, impact resistance, tensile properties, and chemical resistance. These properties were also compared to those of the reference filler. At the same time, the influence of aggressive media on the properties of these materials was observed, in particular on flexural characteristics. The microstructures of the developed coatings were tested using a high-resolution optical microscope, before and after exposure to the chemicals. The positive effect of using progressive fillers, such as solidified hazardous waste (a solidification product (SF)), was witnessed by their constructive contribution to the materials’ physical and mechanical properties. The use of solidification products is unambiguously advantageous from technical, ecological, and economical stand points (utilization of hazardous waste as a progressive filler instead of landfilling, improvement of tensile properties, reduction in the price of coating system, and incorporation of the pollutants into the polymer matrix).
Iterative Learning Control for V-Shaped Electrothermal Microactuator
Authors: Dzung Nguyen Tien , Phuc Pham Hong , Dich Nguyen Quang , Phuoc Nguyen Doan
Published: November 2019

The paper introduces a modified version of a Proportional Integral Derivative (PID)-type iterative learning algorithm, which is very simple to implement on a digital control device for tracking control of a continuous-time system. The simulative application of it is for controlling a V-shaped electrothermal microactuator (VEM) and is carried out by using a Simscape model of VEM for the purpose that the asymptotic tracking behavior of system output to desired trajectory will be verified in a virtually real environment. Obtained simulation results confirm that the introduced iterative learning algorithm has not only provided a good output tracking behavior, as expected, but also is robust in the sense of reducing external disturbance effects.
Transcriptome and DNA Methylation Analyses of the Molecular Mechanisms Underlying with Longissimus dorsi Muscles at Different Stages of Development in the Polled Yak
Authors: Ma Xiaoming , Jia Congjun , Chu Min , Fu Donghai , Lei Qinhui , Ding Xuezhi , Wu Xiaoyun , Guo Xian , Pei Jie , Bao Pengjia , Yan Ping , Liang Chunnian
Published: November 2019

DNA methylation modifications are implicated in many biological processes. As the most common epigenetic mechanism DNA methylation also affects muscle growth and development. The majority of previous studies have focused on different varieties of yak, but little is known about the epigenetic regulation mechanisms in different age groups of animals. The development of muscles in the different stages of yak growth remains unclear. In this study, we selected the longissimus dorsi muscle tissue at three different growth stages of the yak, namely, 90-day-old fetuses (group E), six months old (group M), and three years old (group A). Using RNA-Seq transcriptome sequencing and methyl-RAD whole-genome methylation sequencing technology, changes in gene expression levels and DNA methylation status throughout the genome were investigated during the stages of yak development. Each group was represented by three biological replicates. The intersections of expression patterns of 7694 differentially expressed genes (DEGs) were identified (padj < 0.01, |log2FC| > 1.2) at each of the three developmental periods. Time-series expression profile clustering analysis indicated that the DEGs were significantly arranged into eight clusters which could be divided into two classes (padj < 0.05), class I profiles that were downregulated and class II profiles that were upregulated. Based on this cluster analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that DEGs from class I profiles were significantly (padj < 0.05) enriched in 21 pathways, the most enriched pathway being the Axon guidance signaling pathway. DEGs from the class II profile were significantly enriched in 58 pathways, the pathway most strongly enriched being Metabolic pathway. After establishing the methylation profiles of the whole genomes, and using two groups of comparisons, the three combinations of groups (M-vs.-E, M-vs.-A, A-vs.-E) were found to have 1344, 822, and 420 genes, respectively, that were differentially methylated at CCGG sites and 2282, 3056, and 537 genes, respectively, at CCWGG sites. The two sets of data were integrated and the negative correlations between DEGs and differentially methylated promoters (DMPs) analyzed, which confirmed that TMEM8C, IGF2, CACNA1S and MUSTN1 were methylated in the promoter region and that expression of the modified genes was negatively correlated. Interestingly, these four genes, from what was mentioned above, perform vital roles in yak muscle growth and represent a reference for future genomic and epigenomic studies in muscle development, in addition to enabling marker-assisted selection of growth traits.
Discrete Fracture Network Modelling in Triassic–Jurassic Carbonates of NW Lurestan, Zagros Fold-and-Thrust Belt, Iran
Authors: Massaro Luigi , Corradetti Amerigo , Tramparulo Francesco d’Assisi , Vitale Stefano , Prinzi Ernesto Paolo , Iannace Alessandro , Parente Mariano , Invernizzi Chiara , Morsalnejad Davoud , Mazzoli Stefano
Published: November 2019

In this study, discrete fracture network (DFN) modelling was performed for Triassic–Jurassic analogue reservoir units of the NW Lurestan region, Iran. The modelling was elaborated following a multi-scale statistical sampling of the fracture systems characterising the analysed succession. The multi-scale approach was performed at two different observation scales. At the macro-scale, a digital outcrop analysis was carried out by means of a digital line-drawing based on camera-acquired images, focussing on the distribution of major throughgoing fractures; at the meso-scale, the scan line method was applied to investigate the background fractures of the examined formations. The gathered data were statistically analysed in order to estimate the laws governing the statistical distribution of some key fracture set attributes, namely, spacing, aperture, and height. The collected dataset was used for the DFN modelling, allowing the evaluation of the relative connectivity of the fracture systems and, therefore, defining the architecture and the geometries within the fracture network. The performed fracture modelling, confirmed, once again, the crucial impact that large-scale throughgoing fractures have on the decompartmentalization of a reservoir and on the related fluid flow migration processes. The derived petrophysical properties distribution showed in the models, defined the Kurra Chine Fm. and, especially, the Sehkaniyan Fm. as good-quality reservoir units, whereas the Sarki Fm was considered a poor-quality reservoir unit.
Investigation of Landslides that Occurred in August on the Chengdu–Kunming Railway, Sichuan, China
Authors: Zheng Qian , Shen Shui-Long , Zhou An-Nan , Cai Hao
Published: November 2019

This paper reports on a large-scale landslide with a movement of 48 thousand m3 of soil and rock that occurred in Sichuan, China. This catastrophic landslide occurred in Aidai village, Ganluo County, at 12:44 on 14 August 2019, blocking a section of the railway between Lianghong station and Aidai station. This landslide resulted in 12 deaths and five people missing. This report describes the preliminary investigation, the rescue activity, topographic survey and analysis as well as the main predisposing and triggering factors. The combined effects of steep topography, continuous rainstorms, floods eroding the foothills of the mountain and human activity were the main influencing factors that triggered this landslide. To reduce the possibility of casualties resulting from large geological disasters, such as landslides and mudslides, in this region in the future, some recommendations are proposed to systematically reduce potential human casualties and economic losses.
The Relationship between Emotional Regulation and School Burnout: Structural Equation Model According to Dedication to Tutoring
Authors: Chacón-Cuberos Ramón , Martínez-Martínez Asunción , García-Garnica Marina , Pistón-Rodríguez María Dolores , Expósito-López Jorge
Published: November 2019

School burnout constitutes a current phenomenon which generates diverse negative consequences in the personal and academic lives of students. Given this situation, it is necessary to develop actions that permit us to regulate this harmful mental state and that are administered from within the school context. A descriptive and cross-sectional study is presented that pursues the objective of examining a structural equation model which brings together burnout and emotional regulation. The model assumes that students receive tutoring at school in order to tackle these types of problems. For this, the sample constituted a total of 569 students from the province of Granada (men = 52.3% (n = 298); women = 47.7% (n = 271)). Mean age was reported as 10.39 ± 0.95 years and the School Burnout Inventory (BMI) and the Emotional Regulation Scale were utilized as the principal instruments. As main findings it was observed that students who received one hour of weekly tutoring showed a positive relationship between expressive suppression as a strategy of emotional regulation, cynicism, and exhaustion as consequences of school burnout. In the same way, a direct association existed between burnout-related exhaustion and cognitive repair. Given that significant relationships could not be observed between these variables in students who do not receive tutoring, higher use of emotional regulation was confirmed amongst tutored students when faced with this negative mental state.
Dysregulated Choline, Methionine, and Aromatic Amino Acid Metabolism in Patients with Wilson Disease: Exploratory Metabolomic Profiling and Implications for Hepatic and Neurologic Phenotypes
Authors: Mazi Tagreed A. , Sarode Gaurav V. , Czlonkowska Anna , Litwin Tomasz , Kim Kyoungmi , Shibata Noreene M. , Medici Valentina
Published: November 2019

Wilson disease (WD) is a genetic copper overload condition characterized by hepatic and neuropsychiatric symptoms with a not well-understood pathogenesis. Dysregulated methionine cycle is reported in animal models of WD, though not verified in humans. Choline is essential for lipid and methionine metabolism. Defects in neurotransmitters as acetylcholine, and biogenic amines are reported in WD; however, less is known about their circulating precursors. We aimed to study choline, methionine, aromatic amino acids, and phospholipids in serum of WD subjects. Hydrophilic interaction chromatography-quadrupole time-of-flight mass spectrometry was employed to profile serum of WD subjects categorized as hepatic, neurologic, and pre-clinical. Hepatic transcript levels of genes related to choline and methionine metabolism were verified in the Jackson Laboratory toxic milk mouse model of WD (tx-j). Compared to healthy subjects, choline, methionine, ornithine, proline, phenylalanine, tyrosine, and histidine were significantly elevated in WD, with marked alterations in phosphatidylcholines and reductions in sphingosine-1-phosphate, sphingomyelins, and acylcarnitines. In tx-j mice, choline, methionine, and phosphatidylcholine were similarly dysregulated. Elevated choline is a hallmark dysregulation in WD interconnected with alterations in methionine and phospholipid metabolism, which are relevant to hepatic steatosis. The elevated phenylalanine, tyrosine, and histidine carry implications for neurologic manifestations and are worth further investigation.
Rapid Molecular Identification of Scolytinae (Coleoptera: Curculionidae)
Authors: Albo Jonathan Eric , Marelli Jean-Philippe , Puig Alina Sandra
Published: November 2019

Routine identification of bark and ambrosia beetles is done using morphology. For people lacking the necessary taxonomic knowledge, proper identification of a novel specimen can be challenging and time consuming. This study compares the usefulness of four genetic markers (28S, EF-1a, ITS2, and COI) and five primer pairs (D2F1/D3R2, eflafor1/eflarev1, ets149/efa754, ITS2F/ITS2R, and LCO1490/HCO2198) to identify Scolytinae beetles, and outlines a molecular identification strategy, with results possible in two days. Markers COI and EF-1a were selected based on the ability of the respective primers to amplify DNA from multiple genera (Coptoborus, Xyleborus, Hypothenemus, Theoborus, and Araptus) and the ability of the resulting sequences to provide accurate and unambiguous matches in GenBank. BLASTn analysis of EF-1a sequences (both primer pairs) correctly identified four out of the five genera and COI sequences identified at least one sample of every genus tested and was the only primer pair to correctly identify Araptus specimens. Further, 28S sequences successfully identified Coptoborus, Xyleborus, and Theoborus but not Hypothenemus or Araptus. The low number of EF-1a (1), 28S (7), and ITS2 (0) sequences from Araptus individuals present in GenBank compared with COI (137) is likely the reason that only the latter marker was capable of identifying members of this genus. ITS2 sequences were insufficient to identify any of the samples tested. This study also determined the minimum quantity of DNA that could be used for molecular identification. Primers D2F1 and D3R2, which had the highest rate of amplification in all genera tested, could yield an informative sequence with as little as 0.00048 ng of DNA, however, at least 0.0024 ng was needed for reliable amplification.
Genome-Wide Analysis of the FABP Gene Family in Liver of Chicken (Gallus gallus): Identification, Dynamic Expression Profile, and Regulatory Mechanism
Authors: Wang Zhang , Yue Ya-Xin , Liu Zi-Ming , Yang Li-Yu , Li Hong , Li Zhuan-Jian , Li Guo-Xi , Wang Yan-Bin , Tian Ya-Dong , Kang Xiang-Tao , Liu Xiao-Jun
Published: November 2019

The fatty acid-binding protein (FABP) gene family, which encodes a group of fatty acid-trafficking molecules that affect cellular functions, has been studied extensively in mammals. However, little is known about the gene structure, expression profile, and regulatory mechanism of the gene family in chickens. In the present study, bioinformatics-based methods were used to identify the family members and investigate their evolutionary history and features of gene structure. Real-time PCR combined with in vivo and in vitro experiments were used to examine the spatiotemporal expression pattern, and explore the regulatory mechanism of FABP genes. The results show that nine members of the FABP gene family, which branched into two clusters and shared a conserved FATTYACIDBP domain, exist in the genome of chickens. Of these, seven FABP genes, including FABP1, FABP3-7, and FABP10 were abundantly expressed in the liver of hens. The expression levels of FABP1, FABP3, and FABP10 were significantly increased, FABP5 and FABP7 were significantly decreased, and FABP4 and FABP6 remained unchanged in hens at the peak laying stage in comparison to those at the pre-laying stage. Transcription of FABP1 and FABP3 were activated by estrogen via estrogen receptor (ER) α, whilst FABP10 was activated by estrogen via ERβ. Meanwhile, the expression of FABP1 was regulated by peroxisome proliferator activated receptor (PPAR) isoforms, of which tested PPARα and PPARβ agonists significantly inhibited the expression of FABP1, while tested PPARγ agonists significantly increased the expression of FABP1, but downregulated it when the concentration of the PPARγ agonist reached 100 nM. The expression of FABP3 was upregulated via tested PPARβ and PPARγ agonists, and the expression of FABP7 was selectively promoted via PPARγ. The expression of FABP10 was activated by all of the three tested PPAR agonists, but the expression of FABP4-6 was not affected by any of the PPAR agonists. In conclusion, members of the FABP gene family in chickens shared similar functional domains, gene structures, and evolutionary histories with mammalian species, but exhibited varying expression profiles and regulatory mechanisms. The results provide a valuable resource for better understanding the biological functions of individual FABP genes in chickens.
Lipidomic Profiling Reveals Significant Perturbations of Intracellular Lipid Homeostasis in Enterovirus-Infected Cells
Authors: Yan Bingpeng , Zou Zijiao , Chu Hin , Chan Gabriella , Tsang Jessica Oi-Ling , Lai Pok-Man , Yuan Shuofeng , Yip Cyril Chik-Yan , Yin Feifei , Kao Richard Yi-Tsun , Sze Kong-Hung , Lau Susanna Kar-Pui , Chan Jasper Fuk-Woo , Yuen Kwok-Yung
Published: November 2019

Enterovirus A71 (EV-A71) and coxsackievirus A16 (CV-A16) are the most common causes of hand, foot, and mouth disease. Severe EV-A71 and CV-A16 infections may be associated with life-threatening complications. However, the pathogenic mechanisms underlying these severe clinical and pathological features remain incompletely understood. Lipids are known to play critical roles in multiple stages of the virus replication cycle. The specific lipid profile induced upon virus infection is required for optimal virus replication. The perturbations in the host cell lipidomic profiles upon enterovirus infection have not been fully characterized. To this end, we performed ultra-high performance liquid chromatography–electrospray ionization–quadrupole–time of flight-mass spectrometry (UPLC-ESI-Q-TOF-MS)-based lipidomics to characterize the change in host lipidome upon EV-A71 and CV-A16 infections. Our results revealed that 47 lipids within 11 lipid classes were significantly perturbed after EV-A71 and CV-A16 infection. Four polyunsaturated fatty acids (PUFAs), namely, arachidonic acid (AA), docosahexaenoic acid (DHA), docosapentaenoic acid (DPA), and eicosapentaenoic acid (EPA), were consistently upregulated upon EV-A71 and CV-A16 infection. Importantly, exogenously supplying three of these four PUFAs, including AA, DHA, and EPA, in cell cultures significantly reduced EV-A71 and CV-A16 replication. Taken together, our results suggested that enteroviruses might specifically modulate the host lipid pathways for optimal virus replication. Excessive exogenous addition of lipids that disrupted this delicate homeostatic state could prevent efficient viral replication. Precise manipulation of the host lipid profile might be a potential host-targeting antiviral strategy for enterovirus infection.
Erosion–Corrosion of 30°, 60°, and 90° Carbon Steel Elbows in a Multiphase Flow Containing Sand Particles
Authors: Khan Rehan , Ya Hamdan H. , Pao William , Khan Armaghan
Published: November 2019

Erosion–corrosion in flow changing devices as a result of sand transportation is a serious concern in the hydrocarbon and mineral processing industry. In this work, the flow accelerated erosion–corrosion mechanism of 90°, 60°, and 30° long radius horizontal–horizontal (H–H) carbon steel elbows with an inner diameter of 50.8 mm were investigated in an experimental closed-flow loop. For these geometrical configurations, erosion–corrosion was elucidated for erosive slug flow regimes and the extent of material degradation is reported in detail. Qualitative techniques such as multilayer paint modeling and microscopic surface imaging were used to scrutinize the flow accelerated erosion–corrosion mechanism. The 3D roughness characterization of the surface indicates that maximum roughness appears in downstream adjacent to the outlet of the 90° elbow. Microscopic surface imaging of eroded elbow surfaces disseminates the presence of corrosion pits on the exit regions of the 90° and 60° elbows, but erosion scars were formed on the entry regions of the 30° elbow. Surface characterization and mass loss results indicated that changing the elbow geometrical configuration from a small angle to wide angle significantly changed the mechanical wear mechanism of the tested elbows. Moreover, the maximum erosive location was identified at the top of the horizontally-oriented elbow for slug flow.
Effect of Compound Fields of Ultrasonic Vibration and Applied Pressure on the 3D Microstructure and Tensile Properties of Recycled Al-Cu-Mn-Fe-Si Alloys
Authors: Zhao Yuliang , Lin Bo , Song Dongfu , Zheng Donghai , Sun Zhenzhong , Xie Chunxiao , Zhang Weiwen
Published: November 2019

The effect of compound fields of ultrasonic vibration and applied pressure (UV+AP) on three-dimensional (3D) microstructure and tensile properties of recycled Al-Cu-Mn-Fe-Si alloys was systematically studied using conventional two-dimensional (2D) microscopy, synchrotron X-ray tomography, and tensile test. The properties of UV+AP treated alloys with the pouring temperature of 740, 710 and 680 °C were compared when those alloys achieved after gravity casting. After UV+AP treatment, the alloy with pouring temperature of 710 °C show the smallest grain size. Also, the sizes of Fe-rich phases and Al2Cu are greatly reduced and their 3D morphologies are compacted. The mechanical properties of UV+AP treated alloys are relatively higher than those measured for gravity cast equivalents. This improvement can be explained by the synergistic effect of acoustic cavitation, acoustic streaming, and force-feeding, which resulted in the dendrite fragmentation, uniform solute distribution, and microstructural refinement. The Orowan strengthening and solution strengthening were identified as the main strengthening mechanisms.
Substructure Development and Damage Initiation in a Carbide-Free Bainitic Steel upon Tensile Test
Authors: Taboada Mari Carmen , Iza-Mendia Amaia , Gutiérrez Isabel , Jorge-Badiola Denis
Published: November 2019

Carbide-free bainitic (CFB) steels belong to the family of advanced high strength steels (AHSS) that are struggling to become part of the third-generation steels to be marketed for the automotive industry. The combined effects of the bainitic matrix and the retained austenite confers a significant strength with a remarkable ductility to these steels. However, CFB steels usually show much more complex microstructures that also contain MA (Martensite–Austenite) phase and auto-tempered martensite (ATM). These phases may compromise the ductility of CFB steels. The present work analyzes the substructure evolution during tensile tests in the necking zone, and deepens into the void and crack formation mechanisms and their relationship with the local microstructure. The combination of FEG-SEM imaging, EBSD, and X-ray diffraction has been necessary to characterize the substructure development and damage initiation. The bainite matrix has shown great ductility through the generation of high angle grain boundaries and/or large orientation gradients around voids, which are usually found close to the bainite and MA/auto-tempered martensite interfaces or fragmenting the MA phase. Special attention has been paid to the stability of the retained austenite (RA) during the test, which may eventually be transformed into martensite (Transformation Induced Plasticity, or TRIP effect).
Metabolic Fingerprint of Chronic Obstructive Lung Diseases: A New Diagnostic Perspective
Authors: Tsoukalas Dimitris , Sarandi Evangelia , Thanasoula Maria , Docea Anca Oana , Tsilimidos Gerasimos , Calina Daniela , Tsatsakis Aristides
Published: November 2019

Chronic obstructive lung disease (COLD) is a group of airway diseases, previously known as emphysema and chronic bronchitis. The heterogeneity of COLD does not allow early diagnosis and leads to increased morbidity and mortality. The increasing number of COLD incidences stresses the need for precision medicine approaches that are specific to the patient. Metabolomics is an emerging technology that allows for the discrimination of metabolic changes in the cell as a result of environmental factors and specific genetic background. Thus, quantification of metabolites in human biofluids can provide insights into the metabolic state of the individual in real time and unravel the presence of, or predisposition to, a disease. In this article, the advantages of and potential barriers to putting metabolomics into clinical practice for COLD are discussed. Today, metabolomics is mostly lab-based, and research studies with novel COLD-specific biomarkers are continuously being published. Several obstacles in the research and the market field hamper the translation of these data into clinical practice. However, technological and computational advances will facilitate the clinical interpretation of data and provide healthcare professionals with the tools to prevent, diagnose, and treat COLD with precision in the coming decades.
Biomimetic Anti-Adhesive Surface Microstructures on Electrosurgical Blade Fabricated by Long-Pulse Laser Inspired by Pangolin Scales
Authors: Li Chen , Yang Yong , Yang Lijun , Shi Zhen
Published: November 2019

The electrosurgical blade is the most common invasive surgical instrument in a cutting and hemostasis process; however, the blade easily leads to the adhesion of overheated soft tissues on the blades and induces a potential danger for the patients. To minimize the adhesive tissues, we proposed the one-step surface texturing method to fabricate anti-adhesive biomimetic scales on stainless steel 316L rapidly based on the self-organized surface microstructures induced by the long-pulse fiber laser, which was inspired by the excellent performances of anti-adhesion and anti-friction in the pangolin scales. The optimal formation parameters, chemical components, and crystal structures of the laser-induced self-organized surface microstructures were investigated in the experiments. Moreover, the underlying formation mechanism was revealed. The electrosurgical blades with biomimetic scales have hydrophobicity and a smaller frictional coefficient, which effectively reduced the adhesion of soft tissue.
Effect of ZnO Nanoparticles Coating Layers on Top of ZnO Nanowires for Morphological, Optical, and Photovoltaic Properties of Dye-Sensitized Solar Cells
Authors: Saleem Muhammad , Farooq W. A. , Khan M. I. , Akhtar Majid. Niaz. , Rehman Saif Ur , Ahmad Naseeb , Khalid Muhammad , Atif M. , AlMutairi Mona A. , Irfan Muhammad
Published: November 2019

This paper reports on the synthesis of ZnO nanowires (NWs), as well asthe compound nanostructures of nanoparticles (NPs) and nanowires (NWs+NPs) with different coating layers of NPs on the top of NWs and their integration in dye-sensitized solar cells (DSSCs). In compound nanostructures, NWs offer direct electrical pathways for fast electron transfer, and the NPs of ZnOdispread and fill the interstices between the NWs of ZnO, offering a huge surface area for enough dye anchoring and promoting light harvesting. A significant photocurrent density of 2.64 mA/cm2 and energy conversion efficiency of 1.43% was obtained with NWs-based DSSCs. The total solar-to-electric energy conversion efficiency of the NWs+a single layer of NPs was found to be 2.28%, with a short-circuit photocurrent density (JSC) of 3.02 mA/cm2, open-circuit voltage (VOC) of 0.74 V, and a fill factor (FF) of 0.76, which is 60% higher than that of NWs cells and over 165% higher than NWs+a triple layer of NPs-based DSSCs. The improved performance was obtained due to the increased specific surface area for higher dye anchoring and light harvesting of compound nanostructures with NWs+a single layer of NPs.
Sintering Behavior and Technological Properties of Low-Temperature Porcelain Tiles Prepared Using a Lithium Ore and Silica Crucible Waste
Authors: Peng Lihua , Qin Shan
Published: November 2019

Porcelain tiles are a building material that has been widely used in recent years and that consumes substantial resources during the sintering process. This study reports on the production of low-temperature porcelain tiles by using low-grade lithium ore (LO) and silica crucible waste (SCW) in a new SiO2–Al2O3–Na2O–K2O–Li2O system. The firing temperature of the porcelain tiles was reduced from 1260 °C to 1070 °C by adding 30% LO instead of feldspar in a modified triaxial ceramic body, and SCW was recycled and used as a raw material. These actions help to reduce the carbon emissions produced during sintering and achieve sustainable development. The effect of phase transitions on the sintering and technological properties of the porcelain tiles was studied by quantitative phase analysis, using X-ray diffraction (XRD). Secondary mullite (0–19%) can be formed at 1040–1100 °C, where more quartz and cristobalite will be retained, which increases the rupture modulus of the porcelain tiles. While the vitreous phase increases rapidly at 1100–1160 °C, the closed pores (0.1–33.1%) will simultaneously expand, causing a decrease in compactness. The results show that low-grade LO (with a cost similar to that of feldspar) allows for the production of porcelain tiles with better process performance at lower temperatures (≤1100 °C).
Dietary Patterns and Components in Nonalcoholic Fatty Liver Disease (NAFLD): What Key Messages Can Health Care Providers Offer?
Authors: Riazi Kiarash , Raman Maitreyi , Taylor Lorian , Swain Mark G. , Shaheen Abdel Aziz
Published: November 2019

Nonalcoholic fatty liver disease (NAFLD) is a rising epidemic worldwide and will be the leading cause of cirrhosis, hepatocellular carcinoma, and liver transplant within the next decade. NAFLD is considered as the hepatic manifestation of metabolic syndrome. Behaviors, such as a sedentary lifestyle and consuming a Western diet, have led to substantial challenges in managing NAFLD patients. With no curative pharmaceutical therapies, lifestyle modifications, including dietary changes and exercise, that ultimately lead to weight loss remain the only effective therapy for NAFLD. Multiple diets, including low-carbohydrate, low-fat, Dietary Approaches to Stop Hypertension (DASH), and Mediterranean (MD) diets, have been evaluated. NAFLD patients have shown better outcomes with a modified diet, such as the MD diet, where patients are encouraged to increase the consumption of fruits and vegetables, whole grains, and olive oil. It is increasingly clear that a personalized approach to managing NAFLD patients, based on their preferences and needs, should be implemented. In our review, we cover NAFLD management, with a specific focus on dietary patterns and their components. We emphasize the successful approaches highlighted in recent studies to provide recommendations that health care providers could apply in managing their NAFLD patients.