bluuebunny/crossref_metadata_2025 huggingface dataset:
Managing overactive bladder in primary care
Authors: Robinson Dudley , Belal Mohammed , Granitsiotis Paraskeve , Hamid Rizwan , Toozs‐Hobson Philip , Sahai Arun
Published: July 2017

As the understanding of the mechanisms that underlie the overactive bladder have increased, so its diagnosis and management have become more complex. This article, based on a consensus group meeting, provides guidance on the current management of overactive bladder in primary care.
Pheochromocytoma with Synchronous Ipsilateral Adrenal Cortical Adenoma
Authors: Hasassri M. Earth , Pandian T. K. , Bobr Aleh A. , Bancos Irina , Young William F. , Richards Melanie L. , Farley David R. , Thompson Geoffrey B. , McKenzie Travis J.
Published: July 2017

Background

Pheochromocytoma with synchronous ipsilateral adrenal cortical adenoma (PSCA) may present with mixed clinical, biochemical, and radiological features characteristic to each neoplasm subtype.

Methods

All patients with a pathological diagnosis of pheochromocytoma were evaluated for an ipsilateral cortical adenoma from 1994 through 2015. Retrospectively extracted data included indications for adrenalectomy, diagnostic workup (biochemical and radiographic), operative characteristics, pathological findings, and postoperative complications.

Results

Sixteen of 413 patients (4%) undergoing adrenalectomy for pheochromocytoma had a PSCA. Median patient age was 57.7 years (IQR 50.1, 63.1); 50% were male. On imaging, 75% of the adrenal neoplasms were found incidentally and only 50% were reported to have a synchronous ipsilateral neoplasm based on imaging findings. Clinically important cortical hormone secretion was diagnosed in 38% of these patients; 25% had glucocorticoid secretory autonomy; and 13% had primary aldosteronism.

Conclusion

Physicians should be aware that adrenal neoplasms with mixed diagnostic findings may represent PSCA. Evaluation should be performed on this co‐occurrence to prevent perioperative complications from resection of an unexpected secretory cortical neoplasm.
The Role of Computed Tomography in the Diagnosis of Necrotizing Soft Tissue Infections
Authors: Martinez Myriam , Peponis Thomas , Hage Aglaia , Yeh Daniel D. , Kaafarani Haytham M. A. , Fagenholz Peter J. , King David R. , de Moya Marc A. , Velmahos George C.
Published: July 2017

Background

The exact role of IV contrast‐enhanced computed tomography (CT) in the diagnosis of necrotizing soft tissue infections (NSTIs) has not yet been established. We aimed to explore the role of CT in patients with clinical suspicion of NSTI and assess its sensitivity and specificity for NSTI.

Methods

The medical records of patients admitted between 2009 and 2016, who received IV contrast‐enhanced CT to rule out NSTI, were reviewed. CT was considered positive in case of: (a) gas in soft tissues, (b) multiple fluid collections, (c) absence or heterogeneity of tissue enhancement by the IV contrast, and (d) significant inflammatory changes under the fascia. NSTI was confirmed only by the presence of necrotic tissue during surgical exploration. NSTI was considered absent if surgical exploration failed to identify necrosis, or if the patient was successfully treated non‐operatively.

Results

Of the 184 patients, 17 had a positive CT and hence underwent surgical exploration with NSTI being confirmed in 13 of them (76%). Of the 167 patients that had a negative CT, 38 (23%) underwent surgical exploration due to the high clinical suspicion for NSTI and were all found to have non‐necrotizing infections; the remaining 129 (77%) were managed non‐operatively with successful resolution of symptoms. The sensitivity of CT in identifying NSTI was 100%, the specificity 98%, the positive predictive value 76%, and the negative predictive value 100%.

Conclusions

A negative IV contrast‐enhanced CT scan can reliably rule out the need for surgical intervention in patients with initial suspicion of NSTI.
The Incidence of Perioperative Cardiac Events after Orthopedic Surgery: A Single Institutional Experience of Cases Performed over One Year
Authors: Urban Michael K. , Wolfe Steffan W. , Sanghavi Neil M. , Fields Kara , Magid Steven K.
Published: July 2017

Background

Orthopedic patients with ischemic heart disease are at risk for postoperative cardiac complications.

Questions/Purposes

Using information from two medical information retrieval systems which insured the capture of all events for the period of study, our goals were to determine the incidence of myocardial injury in at-risk patients after orthopedic surgery and to delineate the type and incidence of cardiac complications in this population.

Methods

For one year, at an orthopedic hospital, we identified all postoperative patients with a measured cTnI level using an electronic ordering system. Preoperative cardiac risk factors and postoperative cardiac complications were identified in patients undergoing a total hip arthroplasty (THA), total knee arthroplasty (TKA), and posterior spinal fusion (PSF). A postoperative myocardial infarction was defined by a cTnI > 0.1 ng/mL, ECG changes, new echocardiographic regional wall motion abnormalities, and evaluation by a cardiologist. Categorical variables were compared among groups with a Fisher's exact or Chi-square test. Continuous variables were compared among groups with ANOVA or the Kruskal-Wallis test. The associations of cardiac risk factors with myocardial injury are expressed as odds ratios from logistic regression models.

Results

During a one-year period, from 10, 627 inpatient orthopedic procedures, 805 patients were identified as at risk for postoperative myocardial ischemia. A total of 20.6% (166/805) of these patients had elevated serum cTnI levels (cTnI > 0.02 ng/mL), and there were ten documented postoperative MIs (10/805; 1.2%). For the at-risk TKA, THA, or PSF patients, 19% (102/532) had elevated cTnI levels and 31% (32/102) had postoperative cardiac complications, including arrhythmias (56%), congestive heart failure (2%), and MI (1%). Adjusting for sex, age, BMI, cardiac risk factors, and medications (statins and β-blockers), PSF patients had 3.9 times the risk of myocardial injury ( p = 0.003) compared to TKA patients and 4.2 times that of THA patients.

Conclusions

The incidence of postoperative myocardial ischemia after major orthopedic surgery in patients with cardiac risk factors is high (8.7%), but the incidence of documented myocardial infarctions and serious cardiac complications remains low (1.2–2%). Patients with higher postoperative cTnI releases were more likely to have cardiac complications, and some procedures (spinal fusions) placed the patients at a higher risk.
Synthesis and Surface Activity of Cationic Amino Acid‐Based Surfactants in Aqueous Solution
Authors: Greber Katarzyna E.
Published: July 2017

I studied the possibility of using amino acid‐based surfactants as emulsifiers at the same time as preservatives. Fourteen lipopeptides were synthesized employing a solid phase peptide synthesis procedure. All compounds were designed to be positively charged from +1 to +4 and acylated with fatty acid chain—palmitic and miristic. The surface activity of the obtained lipopeptides was tested using a semi‐automatic tensiometer to calculate parameters describing the behavior of lipopeptides in the air/water interface. Such parameters as CMC, surface tension at the CMC point (σCMC), effectiveness (πCMC), and efficiency (pC20) were measured. Emulsifying properties of all lipopeptides were also examined. The studies reveal that the surface active properties of synthesized compounds strongly depend on the length of alkyl chains as well as on the composition of amino acid polar heads. The critical micelle concentration decreases with increasing alkyl chain length of lipopeptides with the same polar head. The effectiveness and efficiency decrease when the number of amino acids in the polar head increases. All lipopeptides established a very weak emulsification power and created unstable water/Miglyol 812 and water/paraffin oil emulsions. Results suggest that lipopeptides cannot be used as emulsifiers; nonetheless, it is possible to use them as auxiliary surfactants with disinfectant properties in combination with more potent emulsifiers.
The Astonishing Population Averted by China’s Birth Restrictions: Estimates, Nightmares, and Reprogrammed Ambitions
Authors: Goodkind Daniel
Published: July 2017

China launched an unprecedented program to control its population in 1971. Experts have dismissed the official estimate of 400 million births averted by this program as greatly exaggerated yet neglect to provide their own estimates. Counterfactual projections based on fertility declines in other countries suggest that China’s program-averted population numbered 360–520 million as of 2015. The low end of this range is based on Vietnam—China’s best national comparator, with a two-child program of its own—and the high end is based on a 16-country comparator selected, ironically, by critics of the official estimate. The latter comparator further implies that China’s one-child program itself averted a population of 400 million by 2015, three-quarters of the total averted population. All such estimates are projected to double by 2060, due mostly to counterfactual population momentum. These and other findings presented herein affirm the astonishing impact of China’s draconian policy choices and challenge the current consensus that rapid socioeconomic progress drove China’s fertility well below two children per family. International comparisons of fertility and income suggest instead that China’s very low fertility arrived two or three decades too soon. If China had not harshly enforced a norm of 1.5-children during the last quarter century, most mothers would have had two children, one-half birth higher than observed.
Willingness to Pay for Retail Location and Product Origin of Christmas Trees
Authors: Zaffou Madiha , Campbell Benjamin
Published: July 2017

Christmas tree sales are considerable throughout the United States. Understanding the drivers of purchase for Christmas trees is critical for producers and stakeholders within states with tree production. Using data from a choice experiment in combination with latent class modeling, we find that tree height is important, but tree species is less important. Further, we show that local labeling does not influence all consumers. With respect to retail location, we show that nursery/greenhouse and choose-and-cut retail outlets are preferred by a majority of consumers but not by all consumers. Recommendations for the varying retail outlets are provided.
Quality improvement primer part 1: Preparing for a quality improvement project in the emergency department
Authors: Chartier Lucas B. , Cheng Amy H.Y. , Stang Antonia S. , Vaillancourt Samuel
Published: July 2017

Emergency medicine (EM) providers work in a fast-paced and often hectic environment that has a high risk for patient safety incidents and gaps in the quality of care. These challenges have resulted in opportunities for frontline EM providers to play a role in quality improvement (QI) projects. QI has developed into a mature field with methodologies that can dramatically improve the odds of having a successful project with a sustainable impact. However, this expertise is not yet commonly taught during professional training. In this first of three articles meant as a QI primer for EM clinicians, we will introduce QI methodology and strategic planning using a fictional case study as an example. We will review how to identify a QI problem, define components of an effective problem statement, and identify stakeholders and core change team members. We will also describe three techniques used to perform root cause analyses—Ishikawa diagrams, Pareto charts and process mapping—and how they relate to preparing for a QI project. The next two papers in this series will focus on the execution of the QI project itself using rapid-cycle testing and on the evaluation and sustainability of QI projects.
We’re All Cultural Historians Now: Revolutions In Understanding Archaeological Theory And Scientific Dating
Authors: Griffiths Seren
Published: July 2017

Radiocarbon dating has had profound implications for archaeological understanding. These have been identified as various “revolutions,” with the latest—Bayesian chronological statistical analyses of large datasets—hailed as a “revolution in understanding.” This paper argues that the full implications of radiocarbon (14C) data and interpretation on archaeological theory have yet to be recognized, and it suggests that responses in Britain to earlier revolutions in archaeological understanding offer salutary lessons for contemporary archaeological practice. This paper draws on the work of David Clarke and Colin Renfrew to emphasize the importance of critical considerations of the relationships between archaeological theory and scientific method, and to emphasize that seemingly neutral aspects of archaeological thought are highly laden interpretatively, and have significant implications for the kinds of archaeology that we write.
MECHANISED MINIMUM SOIL DISTURBANCE ESTABLISHMENT AND YIELD OF DIVERSE CROPS IN PADDY FIELDS USING A TWO-WHEEL TRACTOR-MOUNTED PLANTER SUITABLE FOR SMALLHOLDER CROPPING
Authors: BELL R. W. , HAQUE M. E. , JOHANSEN C. , VANCE W. , KABIR M. E. , MUSA M. A. , MIA M. N. N. , NEOGI M. G. , ISLAM M. A.
Published: July 2017

Shortages of hired labour are leading to greater interest in mechanisation for crop establishment in smallholder agriculture. Due to small field sizes, mechanised planters mounted on four-wheel tractors are not a suitable technology. The Versatile Multi-crop Planter (VMP) was developed for zero tillage (ZT), strip planting (SP) or single pass shallow tillage (SPST) on flat land and for forming and planting on tops of beds, each in a single pass operation, when mounted on a two-wheel tractor (2WT). The aim of the present study was to evaluate the field performance of the VMP in comparison to conventional broadcast seeding and full rotary tillage (2 to 4 passes; called CT) for establishing chickpea (Cicer arietinum L.), jute (Corchorus olitorius L.), lentil (Lens culinaris Medikus), maize (Zea mays L.), mung bean (Vigna radiata L. R. Wilczek), rice (Oryza sativa L.) and wheat (Triticum aestivum L.) in 15 locations of Bangladesh. Plant populations emerging from all single pass operations viz. SP, ZT, and bed planting (BP) were generally satisfactory and in 12 out of 15 experiments plant populations after SP were similar to or greater than after CT. In addition, SP gave comparable or greater plant populations than SPST and BP planting methods. Overall, the SP planting achieved comparable yields and lower costs of establishment than CT. We conclude that effective and reliable planters are now available for sowing a range of crop species on small fields with minimum soil disturbance. This opens up realistic options for the development of mechanised conservation agriculture suited to small field sizes.
The Political Economy of Ending Headhunting in Central Borneo: Inter-colonial and Kenyah perspectives on the 1924 Kapit Peacemaking Agreement and its aftermath
Authors: LUMENTA DAVE
Published: July 2017

This article, largely based on archival research, highlights two contradictory outcomes of colonial state formation in central Borneo. The first is characterized by territorial consolidation and efforts to neatly sedentarize peoples within each colonial territory, while the second is characterized by pacification that unwittingly liberalized the flows and movements of people and commodities transgressing colonial state boundaries. The 1924 Kapit Peacemaking Agreement in colonial Sarawak is often noted for its significance in bringing a final end to the practice of inter-ethnic headhunting, principally between the Iban of Sarawak and the Kenyah from Dutch Borneo. While it marked the successful outcome of a long phase of colonial pacification and territorial consolidation for both colonial states in Borneo, the agreement's outcome simultaneously highlights the contradictory inter-colonial motives and expectations regarding the resulting increase of cross-border flows of people and commodities. The presented case highlights challenges facing Dutch colonial state formation when attempts to subjugate and sedentarize riverine peoples, who were geographically tied to fluid commodity chains and flows, directly undermined the former's own efforts to establish authority in its borderland frontier.
How older people as pedestrians perceive the outdoor environment – methodological issues derived from studies in two European countries
Authors: WENNBERG HANNA , PHILLIPS JUDITH , STÅHL AGNETA
Published: July 2017

This paper has re-analysed and compared data between three studies conducted in the United Kingdom and in Sweden (the OPUS ‘Older People's Use of Unfamiliar Space’ study in the United Kingdom and the Swedish studies ‘Let's Go for a Walk’ and ‘Walking in Old Age’) to provide a comprehensive account of the issues facing older people in the outdoor environment. All three studies draw on the ‘fit’ between the person and their environment as a guiding conceptual base – capturing the dynamics of the relationship between older people's personal needs and their wider environmental context. This common conceptual base allowed us to test theory against practice, and to explore the utility of this concept across different geographical contexts. Participatory research was also applied, highlighting the importance of the voice of older people and involving older people in research. The studies also used a mixed-method approach involving both quantitative and qualitative methods. The paper highlights that although not generalisable, you can compare cross-locales and cross-nationally using different methodology; it investigates the challenges of cross-national comparative analysis and draws on findings from the three studies to illustrate the different challenges and solutions and finally looks at lessons that are transferable.
IMPACT OF HEALTH TECHNOLOGY ASSESSMENT IN LITIGATION CONCERNING ACCESS TO HIGH-COST DRUGS
Authors: Aleman Alicia , Perez Galan Ana
Published: July 2017

Objectives:The impact of health technology assessment (HTA) in the judicialization of the right of health has not been deeply studied in Latin American countries. The purpose of this study is to review the process of judicialization of the access to high cost drugs in Uruguay and assess the impact HTAs have had on this process.

Methods:The methodology used for this study included a comprehensive literature search in electronic databases, local journals, internal documents developed in the Ministry of Health, as well as conducting interviews with key informants.

Results:Judicialization of the access of high cost drugs has been increasing since 2010. The strategy of the Ministry of Health of Uruguay to decrease this problem included the organization of roundtables with judges and other stakeholders on the basis of HTA, the training of defense lawyers in the use and interpretation of HTA, and the participation of a professional who develops HTA in the preparation of the defense arguments. A year after the implementation of this strategy, 25 percent of writs of protection were won by the Ministry of Health.

Conclusions:Even though the strategy implemented was effective in reducing the loss of litigations, it was not effective in reducing the growing number of writs of protection. It is essential to address this problem in a broad debate and to promote understanding between the parties.
CURRENT ENVIRONMENT FOR INTRODUCING HEALTH TECHNOLOGY ASSESSMENT IN GREECE
Authors: Kani Chara , Kourafalos Vasilios , Litsa Panagiota
Published: July 2017

Objectives: The aim of this study was to describe the current regulatory environment in Greece to evaluate the potential introduction of health technology assessment (HTA) for medicinal products for human use.

Methods: Data sources consist of national legislation on pricing and reimbursement of health technologies to identify the potential need of establishing HTA and its relevant structure.

Results: The pricing procedure regarding medicinal products for human use is based on an external reference pricing mechanism which considers the average of the three lowest Euorpean Union prices. Currently, a formal HTA procedure has not been applied in Greece, and the only prerequisite used for the reimbursement of medicinal products for human use is their inclusion in the Positive Reimbursement List. To restrict pharmaceutical expenditure, a variety of measures—such as clawback mechanisms, rebates, monthly budget caps per physician, generics penetration targeting—have been imposed, aiming mainly to regulate the price level rather than control the introduction of medicinal products for human use in the Greek pharmaceutical market.

Conclusions: Greece has the opportunity to rapidly build capacity, implement, and take advantage of the application of HTA mechanisms by clearly defining the goals, scope, systems, context, stakeholders, and methods that will be involved in the local HTA processes, taking into account the country's established e-prescription system and the recently adapted legislative framework.
SMARTPHONE REMINDER FOR PHYSICAL ACTIVITY IN PEOPLE WITH INTELLECTUAL DISABILITIES
Authors: Pérez-Cruzado David , Cuesta-Vargas Antonio Ignacio
Published: July 2017

Several studies have been carried out regarding different interventions in an effort to improve levels of physical activity in people with an intellectual disability (ID). Studies have been carried out regarding physical activity interventions (1), educational interventions (2), and multimodal interventions (3). New technologies are currently being used to carry out changes in daily habits and to promote health in different pathologies but not in people with an ID (4;5). Due to the poor maintenance of physical activity that we have found in people with ID, the objective of the present study is to determine the maintenance of levels of physical activity, quality of life, self-efficacy, and social support in this population through an intervention with an application in their smartphones.
THE STOMATAL COMPLEX OF PODOCARPUS OBSERVED IN CROSS-SECTION USING CRYOFRACTURE: A PRELIMINARY STUDY
Authors: Whiting M. , Mill R. R. , Jeffree C. E.
Published: July 2017

Cryofracture of living material and fracture at room temperature of herbarium material were used to obtain cross-sections of the stomatal complexes of four species of Podocarpus (Podocarpaceae) for scanning electron microscopy. Cross-sections of the stomata of one species in Podocarpus subgenus Foliolatus section Foliolatus (Podocarpus rubens), one in Podocarpus section Globulus (Podocarpus beecherae), one in Podocarpus subgenus Foliolatus section Longifoliolatus (Podocarpus insularis) and one in subgenus Podocarpus section Australis (Podocarpus nivalis) were studied. The architecture of the stomatal complex, including the wax plug, is described. It was found that the wax plug sits high in the stomatal antechamber in Podocarpus rubens, P. beecherae and Podocarpus decipiens and about halfway up the chamber in P. nivalis. A ridge, which appears to correspond to the crease where the guard cells meet, exists on the underside of the wax plug in Podocarpus beecherae, P. decipiens and P. rubens; its presence in P. nivalis requires confirmation. In addition, ridges within the stomatal antechamber were observed when viewing the cross-sections of Podocarpus decipiens and P. rubens, the internal surface of the cuticle of P. decipiens, Podocarpus teysmannii, P. insularis and Podocarpus milanjianus, and the external surface of the cuticle of Podocarpus chinensis, Podocarpus macrophyllus and Podocarpus pilgeri. These ridges may consist of wax and be a result of epitaxis.
The role of encyclopedic world knowledge in semantic transparency intuitions of idioms
Authors: RAMONDA KRIS
Published: July 2017

This article reports on the role pragmatic inferencing plays in accounting for the ways in which native speakers perceive and interpret the semantic transparency of idioms. Although previous studies have suggested that semantic transparency intuitions of idioms are partly motivated by the conceptual metaphors that underlie them (Gibbs 1992; Gibbset al.1997), findings from other studies (Keysar & Bly 1995, 1999) have raised questions concerning the arbitrariness of such intuitions. This study seeks to further address the discussion on the nature of semantic transparency by examining the role of pragmatic inferencing and encyclopedic world knowledge for understanding how native speakers interpret the relationship between the literal parts and figurative meanings of metaphorical idioms. To this end, semantic transparency ratings were elicited among fifteen native speakers of English for 222 metaphorical English idioms. Furthermore, raters provided qualitative support by justifying their ratings for a smaller subset of 30 idioms. These initial results were then triangulated by a follow-up exploratory study surveying etymological notes from a number of idiom dictionaries. The findings suggest that pragmatic inferencing via encyclopedic world knowledge plays an important role for the non-arbitrary way in which native speakers perceive the semantic transparency of idioms.
Factors associated with continued participation in a matched monetary incentive programme at local farmers’ markets in low-income neighbourhoods in San Diego, California
Authors: Ratigan Amanda R , Lindsay Suzanne , Lemus Hector , Chambers Christina D , Anderson Cheryl AM , Cronan Terry A , Browner Deirdre K , Wooten Wilma J
Published: July 2017

Objective

The Farmers’ Market Fresh Fund Incentive Program is a policy, systems and environmental intervention to improve access to fresh produce for participants on governmental assistance in the USA. The current study examined factors associated with ongoing participation in this matched monetary incentive programme.

Design

Relationship of baseline factors with number of Fresh Fund visits was assessed using Poisson regression. Mixed-effects modelling was used to explore changes in consumption of fruits and vegetables and diet quality.

Setting

San Diego, California.

Subjects

Recipients of Supplemental Nutrition Assistance Program (SNAP), Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) and Supplemental Security Income (SSI) who attended participating farmers’ markets from 2010 to 2012 (n 7298).

Results

Among those with participation for ≤6 months, factors associated with increased visits included reporting more daily servings of fruits and vegetables (F&V) at baseline, being Vietnamese or Asian/Pacific Islander, and eligibility because of SNAP/CalFresh or SSI (v. WIC). Among those who came for 6–12 months, being Asian/Pacific Islander, eligibility because of SNAP/CalFresh and enrolling in the autumn, winter or spring were associated with a greater number of Fresh Fund visits. Among those who came for >12 months, being male and eligibility because of SSI were associated with a greater number of visits. Overall, the odds of increasing number of servings of F&V consumed increased by 2 % per month, and the odds of improved perception of diet quality increased by 10 % per month.

Conclusions

Sustaining and increasing Fresh Fund-type programme operations should be a top priority for future policy decisions concerning farmers’ market use in low-income neighbourhoods.
MeCP2, a target of miR-638, facilitates gastric cancer cell proliferation through activation of the MEK1/2–ERK1/2 signaling pathway by upregulating GIT1
Authors: Zhao L Y , Tong D D , Xue M , Ma H L , Liu S Y , Yang J , Liu Y X , Guo B , Ni L , Liu L Y , Qin Y N , Wang L M , Zhao X G , Huang C
Published: July 2017

Methyl-CpG binding protein 2 (MeCP2) is involved in the carcinogenesis and progression of multiple types of cancer. However, its precise role in gastric cancer (GC) and the relevant molecular mechanism remain unknown. In the present study, we found that miR-638 levels were lower in GC tissues and GC cell lines than in adjacent normal tissues and normal gastric epithelial cell lines, respectively. Low miR-638 levels were associated with poor tumor differentiation, tumor size and lymph node metastasis. MeCP2 expression levels were higher in GC tissues than in adjacent normal tissues. It was found that miR-638 inhibited GC cell proliferation, colony formation, G1–S transition and tumor growth, and induced cell apoptosis by directly targeting MeCP2. MeCP2 promoted GC cell proliferation, colony formation and G1–S cell-cycle transition, and suppressed apoptosis. Molecular mechanistic investigations were performed using an integrated approach with a combination of microarray analysis, chromatin immunoprecipitation sequencing and a reporter gene assay. The results showed that MeCP2 bound to the methylated CpG islands of G-protein-coupled receptor kinase-interacting protein 1 (GIT1) promoter and upregulated its expression, thereby activating the MEK1/2–ERK1/2 signaling pathway and promoting GC cell proliferation. Taken together, our study demonstrates that MeCP2, a target of miR-638, facilitates GC cell proliferation and induces cell-cycle progression through activation of the MEK1/2–ERK1/2 signaling pathway by upregulating GIT1. The findings suggest that MeCP2 plays a significant role in GC progression, and may serve as a potential target for GC therapy.
MALT1 promotes melanoma progression through JNK/c-Jun signaling
Authors: Wang Y , Zhang G , Jin J , Degan S , Tameze Y , Zhang J Y
Published: July 2017

Mucosa-associated lymphoma antigen 1 (MALT1) is a lymphoma oncogene that regulates signal transduction as a paracaspase and an adaptor protein. Yet, the role of MALT1 in other solid cancers such as melanoma is not well-understood. Here, we demonstrate that MALT1 is overexpressed in malignant melanoma cells, and predicts a poor disease-free survival. MALT1 inhibition via shRNA-mediated gene silencing or pharmacologically with MI-2 compound markedly reduced cell growth and migration of A2058 and A375 melanoma cell lines in vitro. Subcutaneous tumor growth analysis revealed that MALT1 gene silencing significantly reduced tumor growth and metastasis to the lung. Consistently, the subcutaneous tumors with MALT1 loss had increased cell apoptosis and decreased proliferation. In addition, these tumors showed signs of mesenchymal–epithelial transition as indicated by the upregulation of E-cadherin and downregulation of N-cadherin and β1-intergrin. Further molecular analysis revealed that MALT1 is required for c-Jun and nuclear factor-κB (NF-κB) activation by tumor necrosis factor-α. Forced expression of the c-Jun upstream activator MKK7 reversed the cell growth and migration defects caused by MALT1 loss. In contrast, NF-κB activation via expression of p65ER, a fusion protein containing NF-κB p65 and the tamoxifen-responsive mutant estrogen receptor, induced minimal effects on cell proliferation, but diminished cell death induced by MALT1 loss and TRAIL treatment. Together, these findings demonstrate that MALT1 promotes melanoma cell proliferation and motility through JNK/c-Jun, and enhances melanoma cell survival through NF-κB, underscoring MALT1 as a potential therapeutic target and biomarker for malignant melanoma.
In situ atomic-scale observation of oxygen-driven core-shell formation in Pt3Co nanoparticles
Authors: Dai Sheng , You Yuan , Zhang Shuyi , Cai Wei , Xu Mingjie , Xie Lin , Wu Ruqian , Graham George W. , Pan Xiaoqing
Published: July 2017

The catalytic performance of core-shell platinum alloy nanoparticles is typically superior to that of pure platinum nanoparticles for the oxygen reduction reaction in fuel cell cathodes. Thorough understanding of core-shell formation is critical for atomic-scale design and control of the platinum shell, which is known to be the structural feature responsible for the enhancement. Here we reveal details of a counter-intuitive core-shell formation process in platinum-cobalt nanoparticles at elevated temperature under oxygen at atmospheric pressure, by using advanced in situ electron microscopy. Initial segregation of a thin platinum, rather than cobalt oxide, surface layer occurs concurrently with ordering of the intermetallic core, followed by the layer-by-layer growth of a platinum shell via Ostwald ripening during the oxygen annealing treatment. Calculations based on density functional theory demonstrate that this process follows an energetically favourable path. These findings are expected to be useful for the future design of structured platinum alloy nanocatalysts.
The Influence of Porosity on Fatigue Crack Initiation in Additively Manufactured Titanium Components
Authors: Tammas-Williams S. , Withers P. J. , Todd I. , Prangnell P. B.
Published: July 2017

Without post-manufacture HIPing the fatigue life of electron beam melting (EBM) additively manufactured parts is currently dominated by the presence of porosity, exhibiting large amounts of scatter. Here we have shown that the size and location of these defects is crucial in determining the fatigue life of EBM Ti-6Al-4V samples. X-ray computed tomography has been used to characterise all the pores in fatigue samples prior to testing and to follow the initiation and growth of fatigue cracks. This shows that the initiation stage comprises a large fraction of life (>70%). In these samples the initiating defect was often some way from being the largest (merely within the top 35% of large defects). Using various ranking strategies including a range of parameters, we found that when the proximity to the surface and the pore aspect ratio were included the actual initiating defect was within the top 3% of defects ranked most harmful. This lays the basis for considering how the deposition parameters can be optimised to ensure that the distribution of pores is tailored to the distribution of applied stresses in additively manufactured parts to maximise the fatigue life for a given loading cycle.
Glargine and degludec: Solution behaviour of higher dose synthetic insulins
Authors: Adams Gary G. , Alzahrani Qushmua , Jiwani Shahwar I. , Meal Andrew , Morgan Paul S. , Coffey Frank , Kok Samil , Rowe Arthur J. , Harding Stephen E. , Chayen Naomi , Gillis Richard B.
Published: July 2017

Single, double and triple doses of the synthetic insulins glargine and degludec currently used in patient therapy are characterised using macromolecular hydrodynamic techniques (dynamic light scattering and analytical ultracentrifugation) in an attempt to provide the basis for improved personalised insulin profiling in patients with diabetes. Using dynamic light scattering and sedimentation velocity in the analytical ultracentrifuge glargine was shown to be primarily dimeric under solvent conditions used in current formulations whereas degludec behaved as a dihexamer with evidence of further association of the hexamers (“multi-hexamerisation”). Further analysis by sedimentation equilibrium showed that degludec exhibited reversible interaction between mono- and-di-hexamer forms. Unlike glargine, degludec showed strong thermodynamic non-ideality, but this was suppressed by the addition of salt. With such large injectable doses of synthetic insulins remaining in the physiological system for extended periods of time, in some case 24–40 hours, double and triple dose insulins may impact adversely on personalised insulin profiling in patients with diabetes.
Multiscale correlative tomography: an investigation of creep cavitation in 316 stainless steel
Authors: Slater T. J. A. , Bradley R. S. , Bertali G. , Geurts R. , Northover S. M. , Burke M. G. , Haigh S. J. , Burnett T. L. , Withers P. J.
Published: July 2017

Creep cavitation in an ex-service nuclear steam header Type 316 stainless steel sample is investigated through a multiscale tomography workflow spanning eight orders of magnitude, combining X-ray computed tomography (CT), plasma focused ion beam (FIB) scanning electron microscope (SEM) imaging and scanning transmission electron microscope (STEM) tomography. Guided by microscale X-ray CT, nanoscale X-ray CT is used to investigate the size and morphology of cavities at a triple point of grain boundaries. In order to understand the factors affecting the extent of cavitation, the orientation and crystallographic misorientation of each boundary is characterised using electron backscatter diffraction (EBSD). Additionally, in order to better understand boundary phase growth, the chemistry of a single boundary and its associated secondary phase precipitates is probed through STEM energy dispersive X-ray (EDX) tomography. The difference in cavitation of the three grain boundaries investigated suggests that the orientation of grain boundaries with respect to the direction of principal stress is important in the promotion of cavity formation.
Mutations Driving Airborne Transmission of A/H5N1 Virus in Mammals Cause Substantial Attenuation in Chickens only when combined
Authors: Richard Mathilde , Herfst Sander , van den Brand Judith M. A. , de Meulder Dennis , Lexmond Pascal , Bestebroer Theo M. , Fouchier Ron A. M.
Published: July 2017

A/H5N1 influenza viruses pose a threat to human and animal health. A fully avian A/H5N1 influenza virus was previously shown to acquire airborne transmissibility between ferrets upon accumulation of five or six substitutions that affected three traits: polymerase activity, hemagglutinin stability and receptor binding. Here, the impact of these traits on A/H5N1 virus replication, tissue tropism, pathogenesis and transmission was investigated in chickens. The virus containing all substitutions associated with transmission in mammals was highly attenuated in chickens. However, single substitutions that affect polymerase activity, hemagglutinin stability and receptor binding generally had a small or negligible impact on virus replication, morbidity and mortality. A virus carrying two substitutions in the receptor-binding site was attenuated, although its tissue tropism in chickens was not affected. This data indicate that an A/H5N1 virus that is airborne-transmissible between mammals is unlikely to emerge in chickens, although individual mammalian adaptive substitutions have limited impact on viral fitness in chickens.
Conditional knock out of N-WASP in keratinocytes causes skin barrier defects and atopic dermatitis-like inflammation
Authors: Kalailingam Pazhanichamy , Tan Hui Bing , Jain Neeraj , Sng Ming Keat , Chan Jeremy Soon Kiat , Tan Nguan Soon , Thanabalu Thirumaran
Published: July 2017

Neural-Wiskott Aldrich Syndrome Protein (N-WASP) is expressed ubiquitously and regulates actin cytoskeleton remodeling. In order to characterize the role of N-WASP in epidermal homeostasis and cutaneous biology, we generated conditional N-WASP knockout mouse using CK14-cre (cytokeratin 14) to ablate expression of N-WASP in keratinocytes. N-WASPK14KO (N-WASP
fl/fl
; CK14-Cre) mice were born following Mendelian genetics suggesting that N-WASP expression in keratinocytes is not essential during embryogenesis. N-WASPK14KO mice exhibited stunted growth, alopecia, dry and wrinkled skin. The dry skin in N-WASPK14KO mice is probably due to increased transepidermal water loss (TEWL) caused by barrier function defects as revealed by dye penetration assay. N-WASPK14KO mice developed spontaneous inflammation in the neck and face 10 weeks after birth. Histological staining revealed thickening of the epidermis, abnormal cornified layer and extensive infiltration of immune cells (mast cells, eosinophils and T-lymphocytes) in N-WASPK14KO mice skin compared to control mice. N-WASPK14KO mice had higher serum levels of IL-1α, TNF-α, IL-6 and IL-17 compared to control mice. Thus our results suggest that conditional N-WASP knockout in keratinocytes leads to compromised skin barrier, higher infiltration of immune cells and hyperproliferation of keratinocytes due to increased production of cytokines highlighting the importance of N-WASP in maintaining the skin homeostasis.
A novel secretion and online-cleavage strategy for production of cecropin A in Escherichia coli
Authors: Wang Meng , Huang Minhua , Zhang Junjie , Ma Yi , Li Shan , Wang Jufang
Published: July 2017

Antimicrobial peptides, promising antibiotic candidates, are attracting increasing research attention. Current methods for production of antimicrobial peptides are chemical synthesis, intracellular fusion expression, or direct separation and purification from natural sources. However, all these methods are costly, operation-complicated and low efficiency. Here, we report a new strategy for extracellular secretion and online-cleavage of antimicrobial peptides on the surface of Escherichia coli, which is cost-effective, simple and does not require complex procedures like cell disruption and protein purification. Analysis by transmission electron microscopy and semi-denaturing detergent agarose gel electrophoresis indicated that fusion proteins contain cecropin A peptides can successfully be secreted and form extracellular amyloid aggregates at the surface of Escherichia coli on the basis of E. coli curli secretion system and amyloid characteristics of sup35NM. These amyloid aggregates can be easily collected by simple centrifugation and high-purity cecropin A peptide with the same antimicrobial activity as commercial peptide by chemical synthesis was released by efficient self-cleavage of Mxe GyrA intein. Here, we established a novel expression strategy for the production of antimicrobial peptides, which dramatically reduces the cost and simplifies purification procedures and gives new insights into producing antimicrobial and other commercially-viable peptides.
Supplemental thymol and carvacrol increases ileum Lactobacillus population and reduces effect of necrotic enteritis caused by Clostridium perfringes in chickens
Authors: Yin Dafei , Du Encun , Yuan Jianmin , Gao Jinxin , Wang YouLi , Aggrey Samuel E. , Guo Yuming
Published: July 2017

Necrotic enteritis (NE) caused by Clostridium perfringens is one of the most detrimental infectious diseases in poultry. This study examined the effect of blends of essential oils (BEOs) (25% thymol and 25% carvacrol) on NE and bacterial dynamics and functions in chicks challenged with C. perfringens. Chicks were assigned to a Control diet and BEOs diet (Control diet + 120 mg/kg BEOs), were challenged with C. perfringens from days 14 to 20 and were killed on day 21 for assessment. Supplementation with BEOs decreased the mortality, alleviated gut lesions, and decreased the virulence factors of pathogenic bacteria (VF 0073-ClpE, VF0124-LPS, and VF0350-BSH). Lack of supplementation also changed the nutrient and immunological dynamics of host microbiota in responding to C. perfringens infection. Adding BEOs changed the host ileum microbial population by increasing the numbers of Lactobacillus crispatus and Lactobacillus agilis, and decreasing Lactobacillus salivarius and Lactobacillus johnsonii. The functional roles of these changing host bacterial populations coupled with the putative reduced pathogenicity of C. perfringens by BEOs contributed to the reduction in gut lesions and mortality in infected chickens. It suggests that dietary supplementation with BEOs could significantly reduce the impact of NE caused by C. perfringens on broilers.
Boron Triangular Kagome Lattice with Half-Metallic Ferromagnetism
Authors: Kim Sunghyun , Han W. H. , Lee In-Ho , Chang K. J.
Published: July 2017

Based on the first-principles evolutionary materials design, we report a stable boron Kagome lattice composed of triangles in triangles on a two-dimensional sheet. The Kagome lattice can be synthesized on a silver substrate, with selecting Mg atoms as guest atoms. While the isolated Kagome lattice is slightly twisted without strain, it turns into an ideal triangular Kagome lattice under tensile strain. In the triangular Kagome lattice, we find the exotic electronic properties, such as topologically non-trivial flat band near the Fermi energy and half-metallic ferromagnetism, and predict the quantum anomalous Hall effect in the presence of spin-orbit coupling.
Occurrence of temperature spikes at a wetting front during spontaneous imbibition
Authors: Aslannejad Hamed , Terzis Alexandros , Hassanizadeh S. Majid , Weigand Bernhard
Published: July 2017

It is reported that temperature rises at wetting front during water infiltration into soil. The temperature goes back to the background value after passage of water front. Different explanations have been provided for source of energy causing temperature spike. Some have contributed it to heat of condensation released due to condensation of vapor on “dry” solid surface. Some other stated that the heat of wetting or heat of adsorption is responsible for the temperature rise. In this research, we revisited this issue. First, we provide a comprehensive review about occurrence of temperature spike at a wetting front. Then, we report about experiments we performed on the rise of water in dry paper. Using infrared and optical imaging techniques, we could monitor temperature changes in time and space. For all samples maximum temperature rise occurred at the wetting front. The magnitude of temperature spike depended on paper material, thickness, and liquid composition. It was larger for cellulose-fiber-based paper than for plastic-based paper. For a given paper type, thicker samples showed a larger temperature spike. Adding salt to the water caused reduction of temperature spike. It was concluded that replacement of air-solid interface with water-solid interface releases energy, which causes temperature rise.
Broadband terahertz-power extracting by using electron cyclotron maser
Authors: Pan Shi , Du Chao-Hai , Qi Xiang-Bo , Liu Pu-Kun
Published: July 2017

Terahertz applications urgently require high performance and room temperature terahertz sources. The gyrotron based on the principle of electron cyclotron maser is able to generate watt-to-megawatt level terahertz radiation, and becomes an exceptional role in the frontiers of energy, security and biomedicine. However, in normal conditions, a terahertz gyrotron could generate terahertz radiation with high efficiency on a single frequency or with low efficiency in a relatively narrow tuning band. Here a frequency tuning scheme for the terahertz gyrotron utilizing sequentially switching among several whispering-gallery modes is proposed to reach high performance with broadband, coherence and high power simultaneously. Such mode-switching gyrotron has the potential of generating broadband radiation with 100-GHz-level bandwidth. Even wider bandwidth is limited by the frequency-dependent effective electrical length of the cavity. Preliminary investigation applies a pre-bunched circuit to the single-mode wide-band tuning. Then, more broadband sweeping is produced by mode switching in great-range magnetic tuning. The effect of mode competition, as well as critical engineering techniques on frequency tuning is discussed to confirm the feasibility for the case close to reality. This multi-mode-switching scheme could make gyrotron a promising device towards bridging the so-called terahertz gap.