Nanopriming of Soybean Seeds Using a Manganese-Containing Nanocomposite in a Matrix of Sulfated Arabinogalactan
Authors:   Siberian Institute of Plant Physiology and Biochemistry SB RAS, Perfileva A. I. , Zabanova N. S. ,   Siberian Institute of Plant Physiology and Biochemistry SB RAS,   Irkutsk State University
Published: October 2024

The results of studies on the stimulation of soybean seed germination under the influence of nanopriming with a chemically synthesized nanocomposite (NC) based on manganese-containing nanoparticles and a water-soluble polysaccharide arabinogalactan are presented. The study was carried out on the phytopathosystem: soybean seeds Glycine max (L) – phytopathogenic bacterium Pectobacterium carotovorum. Phytopathogenic bacterium P. carotovorum causes the infectious disease soft rot in various plant species from vegetables to trees and shrubs. After nanopriming (treatment Mn(OH)2/AGs NC), seed germination, biometric characteristics (mass and length of the root and hypocotyl) and biochemical parameters of soybean seedlings (content of reactive oxygen species (ROS), activity of antioxidant enzymes, amount of lipid peroxidation products) were analyzed. It was shown that the infection of seeds with P. carotovorum led to a decrease in their germination, 50 % of the seeds did not germinate. In addition, seedlings obtained from infected seeds had reduced all biometric characteristics compared to the control. Infection of seeds with P. carotovorum showed an increase in the amount of ROS in root tissues and caused a significant increase in peroxidase activity in the roots of soybean seedlings. A significant increase in diene conjugates (DC) was noted in the tissues of the roots of the hypocotel in this variant. The nanocomposite of manganese hydroxide and sulfated arabinogalactan (Mn(OH)2/AGs NC, 4,8 % Mn) removed the negative effect of the phytopathogen P. carotovorum. Morphometric characteristics of seed sprouts after nanopriming were characterized by an increase in biomass compared to the control. Thus, after nanopriming with Mn(OH)2/AGs NC, the length and weight of the root in uninfected seeds increased. The most pronounced stimulating effect of nanopriming was expressed in sprouts obtained from infected seeds. The length weight of the hypocotyl and the length of the root in infected seeds significantly increased after nanopriming of infected seeds. The precursor NC – MnSO4×5H2O did not affect the germination and sprouting of seeds both infected with P. carotovorum and free from infection. Nanopriming of infected seeds reduced the formation of ROS in seedling tissues. It was found that after nanopriming, peroxidase activity increased significantly in soybean roots compared to the control. This effect can be explained by the antioxidant effect of Mn NPs. Nanopriming of infected seeds reduced the amount of DC in root tissues to the control level. Thus, the mechanisms of interaction of NC with the studied phytopathosystem are represented by an increase in seed germination under the influence of NC, stimulation of morphometric parameters of seedlings, as well as an effect on the components of the antioxidant system. The obtained results and our previous studies on the antibacterial effect of Mn(OH)2/AGs against phytopathogens Clavibacter sepedonicus demonstrate the great potential of using Mn(OH)2/AGs in practical agriculture, in particular for application in the field of stimulating plant growth and increasing their resistance to phytopathogens.