bluuebunny/crossref_metadata_2025 huggingface dataset:
Postmortem Documentation of SARS-CoV-2 in Utero and Postpartum Transmission, through Amniotic Fluid, Placental, and Pulmonary Tissue RT-PCR
Authors: Enache Alexandra , Ciocan Veronica , Muresan Camelia Oana , Cut Talida Georgiana , Novacescu Dorin , Paul Corina , Andreescu Nicoleta , Mihailescu Alexandra , Raica Marius , Dumache Raluca
Published: October 2021

The physiopathology of SARS-CoV-2 infection, during pregnancy and in early childhood, is poorly understood. Unfavorable maternal outcomes, the risk of vertical/postpartum transmission, and severe, multisystem involvement in infants and children highlight the importance of developing a cohesive treatment and nuanced prophylaxis strategy. In this study, we evaluate autopsy reports, pathological findings, and SARS-CoV-2 genome expression in three distinct clinical scenarios: maternal death due to severe COVID-19 with in utero fetal demise (27 weeks); mother with moderate COVID-19 and in utero fetal demise (29 weeks); and 2-month-old infant death with confirmed COVID-19 caregivers. We report the presence of the SARS-CoV-2 genome in amniotic fluid and placental tissue in the context of in utero transmission of SARS-CoV-2, but also in postmortem infant pulmonary tissue samples in a case of late postpartum SARS-CoV-2 transmission with asymptomatic, rapidly progressive disease, resulting in infant death. Key pathological findings offer a descriptive portrayal of maternal, in utero, and infantile COVID-19 pathogenesis. Further investigations are necessary to fully comprehend the clinical implications of SARS-CoV-2 infection during pregnancy, a prerequisite for adequate therapeutic management and harm reduction.
Laser R-Test for Angular Positioning Calibration and Compensation of the Five-Axis Machine Tools
Authors: Tran Cao-Sang , Hsieh Tung-Hsien , Jywe Wen-Yuh
Published: October 2021

The angular positioning error of the rotary stage causes low quality in milling various angles of a workpiece. This study proposes a solution that could improve these issues by using our Laser R-test for angular positioning calibration and compensation of the five-axis machine tools in compliance with the simultaneous measurement path of ISO regulations: ISO 10791-6 and ISO 230-2. System uncertainty analysis and calibration were implemented for system prediction. The measurement method proposed in this paper could solve concentricity problems between measurement devices and the rotary table by applying the Cosine theorem with a Cartesian coordinate system. Further, we used the commercial instrument XR20-W (Renishaw, UK) rotary axis calibrator to verify and compare the measured results on a CNC machine tool. The applied system achieves an angular error of 0.0121 degrees for actual workpieces and is smaller than the referring commercial system, which achieves an error of about 0.0022 degrees. The system in this research is useful for five-axis machine tool full calibrations.
Down the Rabbit Hole: Fostering Active Learning through Guided Exploration of a SCADA Cyber Range
Authors: Cruz Tiago , Simões Paulo
Published: October 2021

Prior experience from the authors has shown that a heavily theoretical approach for cybersecurity training has multiple shortcomings, mostly due to the demanding and diversified nature of the prerequisites, often involving concepts about operating system design, networking and computer architecture, among others. In such circumstances, the quest for trainee engagement often turns into a delicate balancing act between managing their expectations and providing an adequate progression path. In this perspective, hands-on exercises and contact with high-fidelity environments play a vital part in fostering interest and promoting a rewarding learning experience. Making this possible requires having the ability to design and deploy different use case training scenarios in a flexible way, tailored to the specific needs of classroom-based, blended or e-learning teaching models. This paper presents a flexible framework for the creation of laboratory and cyber range environments for training purposes, detailing the development, implementation and exploration of a cyber range scenario, within the scope of a course on cyber-physical systems security. Moreover, the course structure, curricular aspects and teaching methods are also detailed, as well as the feedback obtained from the students.
Wire Arc Additive and High-Temperature Subtractive Manufacturing of Ti-6Al-4V
Authors: Miyake Ryotaro , Sasahara Hiroyuki , Suzuki Atsushi , Ouchi Seigo
Published: October 2021

We investigated the fabrication and finishing of wall-profile machining by wire and arc additive manufacturing (WAAM) employing plasma welding with Ti-6Al-4V wire. We fabricated and integrated a local shield and a cover for the area below the local shield to achieve higher shielding ability. The tensile strength of the fabricated object met the forging standard for Ti-6Al-4V, but elongation was about 7%. We also focused on the possibility of reducing the cutting force and increasing the efficiency of the finishing process by cutting workpieces softened by high temperature immediately after the deposition process. We investigated the cutting force and tool wear of the fabricated objects heated to 300 °C using ceramics tools. Results showed that although the cutting force was reduced at high temperature, the wear rate of the tools was high, and the increase in cutting force due to wear was significant.
Characterization of PM10 Emission Rates from Roadways in a Metropolitan Area Using the SCAMPER Mobile Monitoring Approach
Authors: Fitz Dennis R. , Bumiller Kurt
Published: October 2021

The SCAMPER mobile system for measuring PM10 emission rates from paved roads was used to characterize emission rates from a wide variety of roads in the Phoenix, AZ metropolitan area. Week-long sampling episodes were conducted in March, June, September, and December. A 180 km-long route was utilized and traveled a total of 18 times. PM10 emission rate measurements were made at 5-s resolution for over 3200 km of roads with a precision of approximately 25%. The PM10 emission rates varied by over two orders of magnitude and were generally low unless the road was impacted with dust deposited by activities such as construction, sand and gravel operations, agriculture, and vehicles traveling on or near unpaved shoulders and roads. The data were tabulated into averages for each of 67 segments that the route was divided into. The segment-averaged PM10 emission rates ranged from zero to 2 mg m−1, with an average of 0.079 mg m−1. There was no significant difference in emission rates between seasons. There was a major drop in emission rates over a weekend, when dust generation activities such as construction are expected to be much reduced. By Monday, the PM10 emission rates had risen to the levels of the previous Friday. This indicates that roads quickly reach an equilibrium PM10 generating potential.
Audiological Performance of ADHEAR Systems in Simulated Conductive Hearing Loss: A Case Series with a Review of the Existing Literature
Authors: Muzzi Enrico , Gambacorta Valeria , Lapenna Ruggero , Pizzamiglio Giulia , Ghiselli Sara , Caregnato Igor , Marchi Raffaella , Ricci Giampietro , Orzan Eva
Published: October 2021

A new non-invasive adhesive bone conduction hearing device (ABCD) has been proposed as an alternative solution for reversible bilateral conductive hearing loss in recurrent or long-lasting forms of otitis media with effusion (OME) in children that cannot undergo surgical treatment. Our aim was to assess the effectiveness of ABCD in children with OME. Twelve normal-hearing Italian-speaking volunteers, in whom a conductive hearing loss was simulated, participated in the study. The free-field average hearing threshold was determined and, to evaluate binaural hearing skills, loudness summation and the squelch effect were assessed. Five conditions were tested: (1) unaided without earplugs, (2) unaided with bilateral earplugs, (3) aided right ear with bilateral earplugs, (4) aided left ear with bilateral earplugs, and (5) bilateral aid with bilateral earplugs. Post-hoc analysis showed a significant statistical difference between plugged, unplugged, and each aided condition. The main results were a better loudness summation and a substantial improvement of the squelch effect in the bilaterally aided. Our results suggest that ABCD is a valid treatment for patients with conductive hearing loss that cannot undergo bone conduction implant surgery. It is also important to consider bilateral aids in order to deal with situations in which binaural hearing is fundamental.
Artificial Intelligence Identifies an Urgent Need for Peripheral Vascular Intervention by Multiplexing Standard Clinical Parameters
Authors: Sonnenschein Kristina , Stojanović Stevan D. , Dickel Nicholas , Fiedler Jan , Bauersachs Johann , Thum Thomas , Kunz Meik , Tongers Jörn
Published: October 2021

Background: Peripheral artery disease (PAD) is a significant burden, particularly among patients with severe disease requiring invasive treatment. We applied a general Machine Learning (ML) workflow and investigated if a multi-dimensional marker set of standard clinical parameters can identify patients in need of vascular intervention without specialized intra–hospital diagnostics. Methods: This is a retrospective study involving patients with stable PAD (sPAD, Fontaine Class I and II, n = 38) and unstable PAD (unPAD, Fontaine Class III and IV, n = 18) in need of invasive therapeutic measures. ML algorithms such as Random Forest were utilized to evaluate a matrix consisting of multiple routinely clinically available parameters (age, complete blood count, inflammation, lipid, iron metabolism). Results: ML has enabled a generation of an Artificial Intelligence (AI) PAD score (AI-PAD) that successfully divided sPAD from unPAD patients (high AI-PAD in sPAD, low AI-PAD in unPAD, cutoff at 50 AI-PAD units). Furthermore, the probability score positively coincided with gold-standard intra-hospital mean ankle-brachial index (ABI). Conclusion: AI-based tools may be promising to enable the correct identification of patients with unstable PAD by using existing clinical information, thus supplementing clinical decision making. Additional studies in larger prospective cohorts are necessary to determine the usefulness of this approach in comparison to standard diagnostic measures.
β-RA Targets Mitochondrial Metabolism and Adipogenesis, Leading to Therapeutic Benefits against CoQ Deficiency and Age-Related Overweight
Authors: Hidalgo-Gutiérrez Agustín , Barriocanal-Casado Eliana , Díaz-Casado María Elena , González-García Pilar , Zenezini Chiozzi Riccardo , Acuña-Castroviejo Darío , López Luis Carlos
Published: October 2021

Primary mitochondrial diseases are caused by mutations in mitochondrial or nuclear genes, leading to the abnormal function of specific mitochondrial pathways. Mitochondrial dysfunction is also a secondary event in more common pathophysiological conditions, such as obesity and metabolic syndrome. In both cases, the improvement and management of mitochondrial homeostasis remain challenging. Here, we show that beta-resorcylic acid (β-RA), which is a natural phenolic compound, competed in vivo with 4-hydroxybenzoic acid, which is the natural precursor of coenzyme Q biosynthesis. This led to a decrease in demethoxyubiquinone, which is an intermediate metabolite of CoQ biosynthesis that is abnormally accumulated in Coq9R239X mice. As a consequence, β-RA rescued the phenotype of Coq9R239X mice, which is a model of primary mitochondrial encephalopathy. Moreover, we observed that long-term treatment with β-RA also reduced the size and content of the white adipose tissue (WAT) that is normally accumulated during aging in wild-type mice, leading to the prevention of hepatic steatosis and an increase in survival at the elderly stage of life. The reduction in WAT content was due to a decrease in adipogenesis, an adaptation of the mitochondrial proteome in the kidneys, and stimulation of glycolysis and acetyl-CoA metabolism. Therefore, our results demonstrate that β-RA acted through different cellular mechanisms, with effects on mitochondrial metabolism; as such, it may be used for the treatment of primary coenzyme Q deficiency, overweight, and hepatic steatosis.
A Gadolinium DO3A Amide m-Phenyl Boronic Acid MRI Probe for Targeted Imaging of Sialated Solid Tumors
Authors: Rajan Christu , Seema Jaya , Chen Yu-Wen , Chen Tsai-Chen , Lin Ming-Huang , Lin Chia-Huei , Hwang Dennis Wen-Han
Published: October 2021

We developed a new probe, Gd-DO3A-Am-PBA, for imaging tumors. Our results showed active targeting of Gd-DO3A-Am-PBA to sialic acid (SA) moieties, with increased cellular labeling in vitro and enhanced tumor accumulation and retention in vivo, compared to the commercial Gadovist. The effectiveness of our newly synthesized probe lies in its adequate retention phase, which is expected to provide a suitable time window for tumor diagnosis and a faster renal clearance, which will reduce toxicity risks when translated to clinics. Hence, this study can be extended to other tumor types that express SA on their surface. Targeting and MR imaging of any type of tumors can also be achieved by conjugating the newly synthesized contrast agent with specific antibodies. This study thus opens new avenues for drug delivery and tumor diagnosis via imaging.
Reflexive Gaze Shifts and Fear Recognition Deficits in Children with Callous-Unemotional Traits and Impulsivity/Conduct Problems
Authors: Muñoz Centifanti Luna C. , Stickle Timothy R. , Thomas Jamila , Falcón Amanda , Thomson Nicholas D. , Gamer Matthias
Published: October 2021

The ability to efficiently recognize the emotions on others’ faces is something that most of us take for granted. Children with callous-unemotional (CU) traits and impulsivity/conduct problems (ICP), such as attention-deficit hyperactivity disorder, have been previously described as being “fear blind”. This is also associated with looking less at the eye regions of fearful faces, which are highly diagnostic. Previous attempts to intervene into emotion recognition strategies have not had lasting effects on participants’ fear recognition abilities. Here we present both (a) additional evidence that there is a two-part causal chain, from personality traits to face recognition strategies using the eyes, then from strategies to rates of recognizing fear in others; and (b) a pilot intervention that had persistent effects for weeks after the end of instruction. Further, the intervention led to more change in those with the highest CU traits. This both clarifies the specific mechanisms linking personality to emotion recognition and shows that the process is fundamentally malleable. It is possible that such training could promote empathy and reduce the rates of antisocial behavior in specific populations in the future.
Development and Validation of Targeted Gene Sequencing Panel Based Companion Diagnostic for Korean Patients with Solid Tumors
Authors: Min Byung-Joo , Lee Woo Seung , Seo Myung-Eui , Lee Kye-Hwa , Jeong Seung-Yong , Ku Ja-Lok , Kim Yeul Hong , Shin Sang-Won , Kim Ju Han
Published: October 2021

Recently, several panels using two representative targeting methods have been developed but they do not reflect racial specificity, especially for Asians. We have developed and analytically validated the Korean Pan-cancer Companion Diagnostic (CDX) Panel to apply targeted anticancer drugs to Korean patients based on the molecular characteristics of tumors using tumor samples without matched patient normal samples. The panel included 31 genes with reported single nucleotide variants, 9 genes with reported copy number variations, and 15 genes with predictive responses to targeted drugs under clinical testing, enabling the panel to be analyzed for the targets of 30 targeted anticancer drugs. It is cost-effective and optimized for cancer type-specific therapy in Korean cancer patients across solid cancer types while minimizing the limitations of existing approaches. In addition, the optimized filtering protocol for somatic variants from tumor-only samples enables researchers to use this panel without matched normal samples. To verify the panel, 241 frozen tumor tissues and 71 formalin-fixed paraffin-embedded (FFPE) samples from several institutes were registered. This gene screening method is expected to reduce test turnaround time and cost, making it a balanced approach to investigate solid cancer-related gene regions.
Which Tyrosine Kinase Inhibitors Should Be Selected as the First-Line Treatment for Chronic Myelogenous Leukemia in Chronic Phase?
Authors: Ono Takaaki
Published: October 2021

With the use of tyrosine kinase inhibitors (TKIs), chronic myelogenous leukemia in chronic phase (CML-CP) has been transformed into a non-fatal chronic disease. Hence, “treatment-free remission (TFR)” has become a possible treatment goal of patients with CML-CP. Currently, four types of TKIs (imatinib, nilotinib, dasatinib, and bosutinib) are used as the first-line treatment for newly diagnosed CML-CP. However, the second-generation TKI (2GTKI), the treatment response of which is faster and deeper than that of imatinib, is not always recommended as the first-line treatment for CML-CP. Factors involved in TKI selection in the first-line treatment of CML-CP include not only patients’ medical background, but also patients’ choice regarding the desired treatment goal (survival or TFR?). Therefore, it is important that clinicians select an appropriate TKI to successfully achieve the desired treatment goal for each patient, while minimizing the development of adverse events. This review compares the pros and cons of using imatinib and 2GTKI for TKI selection as the first-line treatment for CML-CP, mainly considering treatment outcomes, medical history (i.e., desire for pregnancy, aging factor, and comorbidity), and cost. The optimal use of 2GTKIs is also discussed.
Crosstalk between KRAS, SRC and YAP Signaling in Pancreatic Cancer: Interactions Leading to Aggressive Disease and Drug Resistance
Authors: Rozengurt Enrique , Eibl Guido
Published: October 2021

Pancreatic ductal adenocarcinoma (PDAC), the predominant form of pancreatic cancer, remains a devastating disease. The purpose of this review is to highlight recent literature on mechanistic and translational developments that advance our understanding of a complex crosstalk between KRAS, YAP and Src tyrosine kinase family (SFK) in PDAC development and maintenance. We discuss recent studies indicating the importance of RAS dimerization in signal transduction and new findings showing that the potent pro-oncogenic members of the SFK phosphorylate and inhibit RAS function. These surprising findings imply that RAS may not play a crucial role in maintaining certain subtypes of PDAC. In support of this interpretation, current evidence indicates that the survival of the basal-like subtype of PDAC is less dependent on RAS but relies, at least in part, on the activity of YAP/TAZ. Based on current evidence, we propose that SFK propels PDAC cells to a state of high metastasis, epithelial-mesenchymal transition (EMT) and reduced dependence on KRAS signaling, salient features of the aggressive basal-like/squamous subtype of PDAC. Strategies for PDAC treatment should consider the opposite effects of tyrosine phosphorylation on KRAS and SFK/YAP in the design of drug combinations that target these novel crosstalk mechanisms and overcome drug resistance.
Treatment of Patients with Monoclonal Gammopathy of Clinical Significance
Authors: Moreno David F. , Rosiñol Laura , Cibeira María Teresa , Bladé Joan , Fernández de Larrea Carlos
Published: October 2021

Monoclonal gammopathy of undetermined significance (MGUS) is defined as the presence of a monoclonal protein (M-protein) produced by a small amount of plasma cells. The majority of patients remain asymptomatic; however, a fraction of them develop clinical manifestations related to the monoclonal gammopathy despite not fulfilling criteria of multiple myeloma or other lymphoproliferative disorder. These patients constitute an emerging clinical issue coined as monoclonal gammopathy of clinical significance (MGCS). The mechanisms involved are poorly understood, and literature is scarce regarding management. The clinical spectrum involves symptoms related to renal, neurologic, skin, ocular, or bleeding manifestations, requiring a multidisciplinary approach. Treatment strategies rely on the basis of symptomatic disease and the M-protein isotype. In this review, we focus on MGCS other than renal, as the latter was earliest recognized and better known. We review the literature and discuss management from diagnosis to treatment based on illustrative cases from daily practice.
Influence of Bio-Based Surfactants on TiO2 Thin Films as Photoanodes for Electro-Photocatalysis
Authors: Duquet Fanny , Nada Amr Ahmed , Rivallin Matthieu , Rouessac Florence , Villeneuve-Faure Christina , Roualdes Stéphanie
Published: October 2021

Photocatalytic water splitting into hydrogen is considered as one of the key solutions to the current demand for eco-responsible energy. To improve the efficiency and sustainability of this process, the development of a TiO2-based photoanode by adding bio-sourced surfactants to the sol–gel preparation method has been considered. Three different polymeric biosurfactants (GB, GC, and BIO) have been tested, giving rise to three different materials being structurally and morphologically characterized by XRD, Rietveld refinement, BET, SEM, AFM, and XPS, which was completed by light absorption, photocatalytic (Pilkington test), electronic (EIS and C-AFM), and photoelectrochemical (cyclic voltammetry) measurements. Correlations between the structure/morphology of materials and their functional properties have been established. One specific surfactant has been proven as the most suitable to lead to materials with optimized photoelectrochemical performance in direct relation with their photocatalytic properties essentially controlled by their specific surface area.
Activation of mTORC1 by Free Fatty Acids Suppresses LAMP2 and Autophagy Function via ER Stress in Alcohol-Related Liver Disease
Authors: Guo Wei , Zhong Wei , Hao Liuyi , Sun Xinguo , Zhou Zhanxiang
Published: October 2021

Alcohol-related liver disease (ALD) is characterized by accumulation of hepatic free fatty acids (FFAs) and liver injury. The present study aimed to investigate if mechanistic target of rapamycin complex 1 (mTORC1) plays a role in FFA-induced organelle dysfunction, thereby contributing to the development of ALD. Cell studies were conducted to define the causal role and underlying mechanism of FFA-activated mTORC1 signaling in hepatocellular cell injury. C57BL/6J wild-type mice were subjected to chronic alcohol feeding with or without rapamycin to inhibit mTORC1 activation. We revealed that palmitic acid (PA)-induced ER stress and suppression of LAMP2 and autophagy flux were mTORC1-dependent as rapamycin reversed such deleterious effects. C/EBP homologous protein (CHOP) was downstream of ATF4 which partially modulated LAMP2. Supplementation with rapamycin to alcohol-fed mice attenuated mTORC1 activation and ER stress, restored LAMP2 protein, and improved autophagy, leading to amelioration of alcohol-induced liver injury. Induction of mTORC1 signaling and CHOP were also detected in the liver of patients with severe alcoholic hepatitis. This study demonstrates that hepatic FFAs play a crucial role in the pathogenesis of ALD by activating mTORC1 signaling, thereby inducing ER stress and suppressing LAMP2-autophagy flux pathway, which represents an important mechanism of FFA-induced hepatocellular injury.
Dysplasia Epiphysealis Hemimelica (Trevor’s Disease) in Children, Two New Cases: Diagnosis, Treatment, and Literature Review
Authors: Ionescu Adelina , Popescu Bogdan , Neagu Oana , Carp Madalina , Tevanov Iulia , Balanescu Laura , Balanescu Radu Ninel
Published: October 2021

Dysplasia epiphysealis hemimelica (DEH), also known as Trevor’s disease, is a rare nonhereditary skeletal disorder affecting one side of the epiphyses or the epiphyses-equivalents. It is often misdiagnosed for traumatic injuries, infections, or other tumors because of the nonspecific clinical features. The diagnosis is mostly based on radiographic involvement of one half of the epiphysis displaying an overgrowth; it is hard to distinguish between DEH and osteochondroma on the gross hystopathological exam. There are few immunohistochemical markers, as well as genetic tests, for EXT1 and EXT2 gene expression that can reveal a more accurate diagnosis. No evidence of malignant changes has been reported and no hereditary transmission or environmental factor has been incriminated as an etiological factor. The natural history of the disease is continuous growth of the lesions until skeletal maturity. Without treatment, the joint might suffer degenerative modification, and the patient can develop early onset osteoarthritis. In the present paper, we report two new cases of DEH of the ankle. The aim of this paper is to consider Trevor’s disease when encountering tumoral masses in the epiphyses of pediatric patients and to present our treatment approach and results.
Immunotherapy in Extensive-Stage Small Cell Lung Cancer
Authors: El Sayed Rola , Blais Normand
Published: October 2021

Small cell lung cancer (SCLC) remains a poorly understood disease with aggressive features, high relapse rates, and significant morbidity as well as mortality, yet persistently limited treatment options. For three decades, the treatment algorithm of SCLC has been stagnant despite multiple attempts to find alternative therapeutic options that could improve responses and increase survival rates. On the other hand, immunotherapy has been a thriving concept that revolutionized treatment options in multiple malignancies, rendering previously untreatable diseases potentially curable. In extensive stage SCLC, immunotherapy significantly altered the course of disease and is now part of the treatment algorithm in the first-line setting. Nevertheless, the important questions that arise are how best to implement immunotherapy, who would benefit the most, and finally, how to enhance responses.
Impact of Split Dosing the First Rituximab Infusion in Patients with High Lymphocyte Count
Authors: Plante Maude , Garneau Laurence , Laprise-Lachance Magali , Lemieux Pierre , Dorval Michel
Published: October 2021

The most common adverse reactions to rituximab are infusion-related reactions (IRR). We evaluated the efficacy of split dosing the first rituximab infusion over two days to reduce IRR incidence in patients with hematological cancer and a high lymphocyte count. This is a retrospective observational study conducted in two healthcare centers in Quebec, Canada. The study enrolled patients with white blood cell counts ≥25.0 × 109/L who received their first rituximab dose for hematological cancer between December 2007 and May 2020. One healthcare center used asymmetrical split dosing, while the other used symmetrical split dosing. A total of 183 treatment episodes were collected from 143 patients. Among patients who received a fractionated dosing schedule, 42% developed an IRR from the first rituximab infusion compared with 50% for the standard protocol (adjusted relative risk, 0.89; p = 0.540). No significant difference was observed in IRR severity between either groups. However, 24% of patients who received the asymmetrical protocol developed an IRR compared to 68% for the symmetrical protocol (adjusted relative risk, 0.32; p = 0.003). These results suggest that an asymmetrical split dosing could be effective in reducing the incidence of IRR and is preferable to a symmetrical one.
Usefulness of a Fork-Tip Needle in Endoscopic Ultrasound-Guided Fine-Needle Biopsy for Gastric Subepithelial Lesions
Authors: Takasumi Mika , Hikichi Takuto , Hashimoto Minami , Nakamura Jun , Kato Tsunetaka , Kobashi Ryoichiro , Yanagita Takumi , Suzuki Rei , Sugimoto Mitsuru , Sato Yuki , Irie Hiroki , Takagi Tadayuki , Kobayakawa Masao , Hashimoto Yuko , Ohira Hiromasa
Published: October 2021

The sample adequacy and diagnostic accuracy of an endoscopic ultrasound (EUS)-guided fine-needle aspiration (EUS-FNA) for gastric subepithelial lesions (SELs) have been reported to be imperfect. To resolve these issues, a fork-tip needle as an EUS-guided fine-needle biopsy (FNB) needle has been developed. This study was conducted to evaluate the usefulness of a fork-tip needle in an EUS-FNB for gastric SELs. Seventy-nine patients who received an EUS-FNA or FNB using a fork-tip needle for gastric SELs were included in the study. The sample adequacy and diagnostic accuracy were compared between the EUS-FNB with the fork-tip needle group (fork-tip group, n = 13) and the EUS-FNA with FNA needle group (FNA group, n = 66). In addition, a multivariate analysis of the factors influencing diagnostic accuracy was conducted. Regarding sample adequacy, there was no significant difference between the groups (100% vs. 90.9%, respectively; p = 0.582). The diagnostic accuracy of the fork-tip group was numerically higher than that of the FNA group (92.3% vs. 81.8%, respectively; p = 0.682). In a multivariate analysis, the diagnostic accuracy was related to the tumor size and location of the SEL but not to the needle type. In conclusion, this study does not show statistical superiority, but suggests the useful potential of a fork-tip needle.
Soap Film Visualization of a 10 cm-Span Flapping Wing
Authors: Yang Lung-Jieh , Tasupalli Chandrashekhar , Waikhom Reshmi , Panchal Nikhil
Published: October 2021

Flapping wing micro-air-vehicles (FWMAVs) animate the small-space dexterous flight, hovering, and energy-saving characteristics of birds and insects, and are believed to have enlightenment for the development of bionic flight in the future. When designing FWMAVs, detailed unsteady aerodynamic information is required. Besides the computational fluid mechanics (CFD) technology study, the flow visualization is also needed to assist this research. This article innovatively used soap film visualization with high-speed photography to record two kinds of the 2D flow fields laterally and longitudinally, respectively, generated by a flapping wing of 10 cm span. Different from the qualitative comparison of soap film imaging with the conventional smoke tracing method, the subsequent processing of the soap film images was demonstrated. This work explains how to quantify the soap film imaging into lift and thrust forces, and the corresponding results are compared with the wind tunnel force measurement data preliminarily.
Scaling, Complexity, and Design Aspects in Computational Fluid Dynamics
Authors: Wang Sheldon
Published: October 2021

With the availability of more and more efficient and sophisticated Computational Fluid Dynamics (CFD) tools, engineering designs are also becoming more and more software driven. Yet, the insights in temporal and spatial scaling issues are still with us and very often imbedded in complexity and many design aspects. In this paper, with a revisit to a so-called leakage issue in sucker rod pumps prevalent in petroleum industries, the author would like to demonstrate the need to use perturbation approaches to circumvent the multi-scale challenges in CFD with extreme spatial aspect ratios and temporal scales. In this study, the gap size between the outer surface of the plunger and the inner surface of the barrel is measured with a mill (one thousandth of an inch) whereas the plunger axial length is measured with inches or even feet. The temporal scales, namely relaxation times, are estimated with both expansions in Bessel functions for the annulus flow region and expansions in Fourier series when such a narrow circular flow region is approximated with a rectangular one. These engineering insights derived from the perturbation approaches have been confirmed with the use of full-fledged CFD analyses with sophisticated computational tools as well as experimental measurements. With these confirmations, new perturbation studies on the sucker rod leakage issue with eccentricities have been presented. The volume flow rate or rather leakage due to the pressure difference is calculated as a quadratic function with respect to the eccentricity, which matches with the early prediction and publication with comprehensive CFD studies. In short, a healthy combination of ever more powerful modeling tools along with the physics, mathematics, and engineering insights with dimensionless numbers and classical perturbation approaches may provide a balanced and more flexible and efficient strategy in complex engineering designs with the consideration of parametric and phase spaces.
Metabolomics for Crop Breeding: General Considerations
Authors: Litvinov Dmitry Y. , Karlov Gennady I. , Divashuk Mikhail G.
Published: October 2021

The development of new, more productive varieties of agricultural crops is becoming an increasingly difficult task. Modern approaches for the identification of beneficial alleles and their use in elite cultivars, such as quantitative trait loci (QTL) mapping and marker-assisted selection (MAS), are effective but insufficient for keeping pace with the improvement of wheat or other crops. Metabolomics is a powerful but underutilized approach that can assist crop breeding. In this review, basic methodological information is summarized, and the current strategies of applications of metabolomics related to crop breeding are explored using recent examples. We briefly describe classes of plant metabolites, cellular localization of metabolic pathways, and the strengths and weaknesses of the main metabolomics technique. Among the commercialized genetically modified crops, about 50 with altered metabolic enzyme activities have been identified in the International Service for the Acquisition of Agri-biotech Applications (ISAAA) database. These plants are reviewed as encouraging examples of the application of knowledge of biochemical pathways. Based on the recent examples of metabolomic studies, we discuss the performance of metabolic markers, the integration of metabolic and genomic data in metabolic QTLs (mQTLs) and metabolic genome-wide association studies (mGWAS). The elucidation of metabolic pathways and involved genes will help in crop breeding and the introgression of alleles of wild relatives in a more targeted manner.
Stress Levels and Mental Well-Being among Slovak Students during e-Learning in the COVID-19 Pandemic
Authors: Rutkowska Anna , Liska David , Cieślik Błażej , Wrzeciono Adam , Broďáni Jaroslav , Barcalová Miroslava , Gurín Daniel , Rutkowski Sebastian
Published: October 2021

The SARS-CoV-2 virus pandemic has forced far-reaching changes in higher education. Isolation from peers and distance learning have significantly limited interpersonal contacts, which might have affected the mental well-being of students. Therefore, the aim of the study was to investigate the prevalence of depressive symptoms and the level of perceived stress during e-learning among Slovak students and to identify the variables that have the most significant impact on mental health among students. The study included 3051 participants, 1773 women (58%) and 1278 (42%) with a mean age of 22.37 years. The Perceived Stress Scale (PSS-10) and Beck Depression Inventory (BDI-II) were used to measure the severity of stress and depression level. In addition, an author’s survey was used assessing the areas of social life, education skills, economic field, nutrition habits, and drugs. Almost all study participants were characterized by increased stress level and 47% of them were depressed. Moreover, isolation affected women more, especially in terms of social life and economics. It seems necessary to implement appropriate support programs for students, which could have the potential to improve their psychological condition.
Crude Extracts and Alkaloids Derived from Ipomoea-Periglandula Symbiotic Association Cause Mortality of Asian Citrus Psyllid Diaphorina citri Kuwayama (Hemiptera: Psyllidae)
Authors: Chen Xue-Dong , Kaur Navneet , Horton David R. , Cooper W. Rodney , Qureshi Jawwad A. , Stelinski Lukasz L.
Published: October 2021

Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is an important economic pest of citrus crops because it vectors the causal pathogen of huanglongbing (HLB; aka citrus greening). Population suppression of D. citri with insecticides has been disproportionally relied on for HLB management and a greater diversity of more sustainable tools is needed. Periglandula spp. is a fungal endosymbiont (family Clavicipitaceae) that forms a mutualistic relationship with members of plants in family Convolvulaceae. This association results in the production of ergot alkaloids that were previously documented as having psyllicidal properties. We investigated the mortality and behavior of D. citri exposed to crude extracts from morning glories in the plant family Convolvulaceae, as well as synthetic ergot alkaloids. Nymphs and adults were exposed to the crude plant extracts from Periglandula positive species of Convolvulaceae, as well as five synthetic ergot alkaloids. Treatments were prepared by exposing clippings of citrus to 100 ng/µL of crude extract from Periglandula-positive species of Ipomoea (I. imperati, I. leptophylla, I. pandurata and I. tricolor), and Turbina corymbosa, and from one Periglandula-negative species (I. alba) (100 ng/µL). Mortality of adult and nymphal D. citri was significantly higher than the control after exposure to extracts from I. tricolor and I. imperati. The synthetic ergot alkaloids, lysergol (10–100 ng/µL), ergonovine maleate (100 ng/µL), agroclavine (10–100 ng/µL), and ergosine (10–100 ng/µL) increased mortality of D. citri nymphs, while ergosine (100 ng/µL) and agroclavine (100 ng/µL) increased mortality of adults compared to water controls. Fewer D. citri adults settled on plants treated with crude extracts or synthetic ergot alkaloids than on water controls at 48 h after release. D. citri that fed on citrus leaves treated with 10 ng/μL solution of crude extract from the Periglandula-positive species Ipomoea (I. imperati, I. leptophylla, I. pandurata, I. tricolor), and Turbina corymbosa excreted significantly less honeydew compared with a negative water control and extract from Periglandula-negative species (I. alba). Our results indicate that crude extracts and ergot alkaloids exhibit toxic and sub-lethal effects on D. citri that could be useful for management of this pest.
Framework on Performance Management in Automotive Industry: A Case Study
Authors: Lascu Elena , Severin Irina , Lascu Florina Daniela , Gudana Razvan Adrian , Nalbitoru Gabriela , Ignat Nicoleta Daniela
Published: October 2021

The purpose of this research is to identify the risks and deficiencies that affect the performance of companies that provide vehicle after-sales services. Thus, this paper highlights the results of a comparative study based on a questionnaire conducted at the level of six brands in the automotive industry. A model was developed to investigate the factors that affect the global performance of the after-sales sector and the authenticity of the information related to the issue studied. Moreover, based on the collected data, this study evaluates the strategies related to performance management used by the organizations studied. In the end, even if the results showed a score of 81% on the questionnaire, we found that companies that provide vehicle after-sales services have not implemented and do not maintain totally the strategies related to performance management. Consequently, the need for change can be emphasized. Based on the analyzed data in the second part of the paper, we identified deficiencies and risks in terms of the organization, operation and management of the service units. These results confirm that the vehicle repair service has a significant influence on employee and customer satisfaction, on the quality of the vehicles repaired and the repair completion time.
Multiscale Enhanced Sampling Using Machine Learning
Authors: Moritsugu Kei
Published: October 2021

Multiscale enhanced sampling (MSES) allows for an enhanced sampling of all-atom protein structures by coupling with the accelerated dynamics of the associated coarse-grained (CG) model. In this paper, we propose an MSES extension to replace the CG model with the dynamics on the reduced subspace generated by a machine learning approach, the variational autoencoder (VAE). The molecular dynamic (MD) trajectories of the ribose-binding protein (RBP) in both the closed and open forms were used as the input by extracting the inter-residue distances as the structural features in order to train the VAE model, allowing the encoded latent layer to characterize the difference in the structural dynamics of the closed and open forms. The interpolated data characterizing the RBP structural change in between the closed and open forms were thus efficiently generated in the low-dimensional latent space of the VAE, which was then decoded into the time-series data of the inter-residue distances and was useful for driving the structural sampling at an atomistic resolution via the MSES scheme. The free energy surfaces on the latent space demonstrated the refinement of the generated data that had a single basin into the simulated data containing two closed and open basins, thus illustrating the usefulness of the MD simulation together with the molecular mechanics force field in recovering the correct structural ensemble.
A GEMMA-GRAFCET Generator for the Automation Software of Smart Manufacturing Systems
Authors: Castillo Juan Manuel , Barbieri Giacomo , Mejia Alejandro , Hernandez José Daniel , Garces Kelly
Published: October 2021

Within the Industry 4.0 revolution, manufacturing enterprises are transforming to intelligent enterprises constituted by Smart Manufacturing Systems (SMSs). A key capability of SMSs is the ability to connect and communicate with each other through Industrial Internet of Things technologies, and protocols with standard syntax and semantics. In this context, the GEMMA-GRAFCET Methodology (GG-Methodology) provides a standard approach and vocabulary for the management of the Operational Modes (OMs) of SMSs through the automation software, bringing a common understanding of the exchanged data. Considering the lack of tools to implement the methodology, this work introduces an online tool based on Model-Driven Engineering–GEMMA-GRAFCET Generator (GG-Generator)–to specify and generate PLCopen XML code compliant with the GG-Methodology. The proposed GG-Generator is applied to a case study and validated using Virtual Commissioning and Dynamic Software Testing. Due to the consistency obtained between the GG-Methodology and the generated PLC code, the GG-Generator is expected to support the adoption of the methodology, thus contributing to the interoperability of SMSs through the standardization of the automation software for the management of their OMs.
Nonlinear Position Control with Augmented Observer in Brushless DC Motor
Authors: Lee Youngwoo , Kim Wonhee
Published: October 2021

In this paper, position control using both a nonlinear position controller and a current controller with an augmented observer is proposed for a Brushless DC motor. The nonlinear position controller is designed to improve the position tracking performance based on the tracking error dynamics. The current controller is developed to track the desired currents generated from the desired torque, which is calculated based on the nonlinear position controller. The augmented observer is designed to obtain the knowledge of both state variables and disturbance. Closed-loop stability is proven through the Lyapunov theorem. Simulations were performed to evaluate the effectiveness of the proposed method.
Interactions Obtained from Basic Mechanistic Principles: Prey Herds and Predators
Authors: Berardo Cecilia , Bulai Iulia Martina , Venturino Ezio
Published: October 2021

We investigate four predator–prey Rosenzweig–MacArthur models in which the prey exhibit herd behaviour and only the individuals on the edge of the herd are subjected to the predators’ attacks. The key concept is the herding index, i.e., the parameter defining the characteristic shape of the herd. We derive the population equations from the individual state transitions using the mechanistic approach and time scale separation method. We consider one predator and one prey species, linear and hyperbolic responses and the occurrence of predators’ intraspecific competition. For all models, we study the equilibria and their stability and we give the bifurcation analysis. We use standard numerical methods and the software Xppaut to obtain the one-parameter and two-parameter bifurcation diagrams.
Hermite–Hadamard–Mercer-Type Inequalities for Harmonically Convex Mappings
Authors: You Xuexiao , Ali Muhammad Aamir , Budak Hüseyin , Reunsumrit Jiraporn , Sitthiwirattham Thanin
Published: October 2021

In this paper, we prove Hermite–Hadamard–Mercer inequalities, which is a new version of the Hermite–Hadamard inequalities for harmonically convex functions. We also prove Hermite–Hadamard–Mercer-type inequalities for functions whose first derivatives in absolute value are harmonically convex. Finally, we discuss how special means can be used to address newly discovered inequalities.
Vitamin-D Binding Protein Gene Polymorphisms and Serum 25-Hydroxyvitamin-D in a Turkish Population
Authors: Karcıoğlu Batur Lutfiye , Özaydın Ahmet , Maviş Murat Emrah , Gürsu Gökçe Göksu , Harbige Laurence , Hekim Nezih
Published: October 2021

The rs7041 and rs4588 polymorphisms found in the GC gene, encoding vitamin D-binding protein (DBP), have distinct biochemical phenotypes. The aim of this study was to investigate vitamin D parameters with these polymorphisms, in individuals with possible vitamin D deficiency. The most common (49% of the cohort) genotype in rs7041 was GT, especially among individuals with high levels of free 25(OH)D calculated but with low levels of bioavailable 25(OH)D, and in rs4588 it was AC in particular among the individuals with low levels of bioavailable 25(OH)D. The most common phenotypes were Gc1s/2 (35.3%) and Gc1s/1s (31.4%), and Gc1f/1f was rare (5.9%). The variations in free and bioavailable 25(OH)D levels among healthy Turkish individuals may be attributed to the variations in total 25(OH)D as well as GC gene polymorphisms. The Turkish population shares a similarity for allele frequencies of rs7041 with the European population and similarity for allele frequencies of rs4588 with Gujarati Indians, and this may also be important in relation to certain ethnic populations showing associations between vitamin D and COVID-19.
Effect of Nano-Additives on the Strength and Durability Characteristics of Marl
Authors: Mirzababaei Mehdi , Karimiazar Jafar , Sharifi Teshnizi Ebrahim , Arjmandzadeh Reza , Bahmani Sayed Hessam
Published: October 2021

Low bearing capacity soils may pose serious construction concerns such as reduced bearing capacity and excessive hydro-associated volume changes. Proper soil remediation techniques must be planned and implemented before commencing any construction on low bearing capacity soils. Environmentally friendly soil stabilizers are gradually replacing traditional soil stabilizers with high carbon dioxide emissions such as lime and cement. This study investigated the use of an alternative pozzolanic mix of nano-additives (i.e., nano-silica and nano-alumina) and cement to reduce the usage of cement for achieving competent soil stabilization outcomes. A series of unconfined compressive strength (UCS), direct shear, and durability tests were conducted on marl specimens cured for 1, 7, and 28 days stabilized with nano-additives (0.1~1.5%), 3% cement, and combined 3% cement and nano-additives. The UCS and shear strength of stabilized marl increased with nano-additives up to a threshold nano-additive content of 1% which was further intensified with curing time. Nano-additive treated cemented marl specimens showed long durability under the water, while the cemented marl decomposed early. The microfabric inspection of stabilized marl specimens showed significant growth of calcium silicate hydrate (CSH) products within the micro fabric of nano-silica treated marl with reduced pore-spaces within aggregated particles. The results confirmed that nano-additives can replace cement partially to achieve multi-fold improvement in the strength characteristics of the marl.