bluuebunny/crossref_metadata_2025 huggingface dataset:
Rainfall Modifies the Disturbance Effects on Regulating Ecosystem Services in Tropical Forests of Bangladesh
Authors: Sultana Fahmida , Arfin-Khan Mohammed A. S. , Karim Md Rezaul , Mukul Sharif A.
Published: February 2023

Tropical forests are critical for ecosystem functioning and are the major provider of global ecosystem services. The combined effects of climate change and anthropogenic disturbances in the tropics, however, have caused a mass decline in major ecosystem services in recent decades. Nevertheless, most previous studies focused entirely on these disturbances’ effects on forest ecosystem services without taking into consideration the combined effects of climate change and disturbances on key ecosystem services’ indicators. This is more pronounced in Bangladesh, which is one of the countries most adversely affected by global climate change. We investigated anthropogenic disturbances’ effects on regulating ecosystem services under different rainfall regimes in Khadimnagar National Park (KNP) and Satchari National Park (SNP), two major forested, protected areas in Northeast Bangladesh. We considered twelve indicators for five aboveground ecosystem services (i.e., primary production, carbon storage, biodiversity, wildlife habitat, and timber production) and four indicators for four belowground ecosystem services (i.e., organic carbon storage, soil aggregate stability, soil physical support, and soil water regulation) in our study. The effects of disturbances on the indicators of four aboveground ecosystem services (i.e., primary production, tree carbon storage, biodiversity conservation, and timber production) and one belowground ecosystem service (soil physical support) differed in terms of rainfall regimes. Disturbances negatively influenced indicators of primary production (p = 0.03, r = −0.28), tree carbon storage (p = 0.02, r = −0.29), and timber production (p = 0.002, r = −0.39), and positively impacted soil organic carbon storage (p = 0.04, r = 0.27) only under a medium-rainfall regime. Interestingly, a significant positive impact of disturbances on soil physical support (p = 0.001, r = 0.39) was observed under heavy rainfall in our study. In addition, disturbances were significantly associated with biodiversity conservation (p < 0.05) and wildlife habitat quality (p < 0.05) under both rainfall classes. Our findings provide critical insights into the synergistic role of anthropogenic perturbations in regulating ecosystem services with rainfall pattern as a key climatic parameter, which can be useful in restoring large tracts of degraded tropical forests in the United Nations (UN) Decade on Ecosystem Restoration.
The Effect of the Tree Dieback Process on the Mechanical Properties of Pine (Pinus sylvestris L.) Wood
Authors: Malinowski Zbigniew , Kawalerczyk Jakub , Walkiewicz Joanna , Dziurka Dorota , Mirski Radosław
Published: February 2023

As a result of the progressing climate changes, there is an increase in the volume of pine deadwood harvested each year from Polish forests. Its presence is an important part of the forest ecosystem; however, there are some indications that the material obtained from dying trees can be characterized by lower quality and properties. Taking into account the growing issue of tree dieback, the volume of pine wood annually harvested in Poland, and the importance of wooden products from an economic standpoint, preliminary research aimed at recognizing the process and its effect on the mechanical properties was conducted. Model trees in Brzeg Forest District were selected based on the crown defoliation. The properties of wood obtained from trees representing three different categories of soundness were determined according to the relevant standards. Based on the results of density, modulus of elasticity, bending strength, and compressive strength, it was found that there were statistically significant differences in wood quality depending on the condition of the tree. The results were particularly interesting in the case of compressing strength, where a healthy tree of lower density showed a similar strength to a dying tree with a much higher density.
How to Improve the Supply of Quasi-Public Forest Infrastructure When Government Is the Leader: Evidence from Experimental Economics
Authors: Zhang Liying , Wu Chengliang , Hao Yan
Published: February 2023

Forest infrastructure is an important material basis for healthy forests. According to public goods theory, most forest infrastructures are quasi-public goods, with demand exceeding supply, more than one supplier, unclear responsibilities between suppliers, and a resultant free-rider problem. This study explored ways to improve the supply of goods for forest infrastructure when the government—as leader—cooperates with foresters—as followers. Experimental economics were used to explain the factors that influence the behaviour of forest infrastructure quasi-public goods suppliers; to design twelve policy scenarios by communication, information feedback, rewards and punishments, and leadership styles; and to simulate the changes in foresters’ supply behaviour in different scenarios to analyse which policies were conducive to improving the supply of quasi-public forest infrastructures. The results were as follows: communication, rewards and punishments, information feedback, and leadership style reduce foresters’ free-riding behaviour; communication, rewards, and punishment increase supply, and, furthermore, the supply of the scenario with rewards and punishment is 1.792–4.616 times that of the situation without rewards and punishment; without the constraints of rewards and punishment, only feedback information reduces the supply; and the p values of the Mann–Whitney U test for the influence of leadership style on the supply level of forest infrastructure were all greater than 0.05, which indicates that no significant difference exists between leading by words and leading by example in supply improvement. When foresters are able to communicate with each other, reward and punishment exist, and information feedback is provided; hence, the supply of foresters is higher regardless of whether the government leads by words or by example. This study provided policy suggestions on how to improve the supply of quasi-public goods for forestry infrastructure, namely, that the organizer should organize foresters to fully negotiate before raising funds for infrastructure construction; publish, when appropriate, the supply and income of foresters; and formulate appropriate reward and punishment measures.
Genetic Diversity of Five Broadleaved Tree Species and Its Spatial Distribution in Self-Regenerating Stands
Authors: Verbylaitė Rita , Pliūra Alfas , Lygis Vaidotas , Suchockas Vytautas , Jankauskienė Jurga , Labokas Juozas
Published: February 2023

European forest ecosystems are currently subject to various disturbances and shifts in land-use legacies. To be able to forecast the trends and consequences of the changes in genetic diversity following these disturbances, it is of crucial importance to understand the genetic dynamics of natural tree populations. The present study aimed at determining the extent and spatial distribution of genetic diversity in five common broadleaved tree species in Lithuania in both mature (putatively maternal) stands and in natural regeneration (juveniles) of the respective species. The genetic diversity of Quercus robur, Betula pendula, Populus tremula, Alnus glutinosa, and Fraxinus excelsior was assessed using eight nuclear microsatellite loci for each species; 417 samples of regenerating juveniles and 141 samples of putatively maternal trees were analyzed in total. The investigated populations of self-regenerating Q. robur, B. pendula, A. glutinosa, and F. excelsior juveniles showed spatially random genetic structures, while P. tremula regenerated mostly via root suckers and formed clonal groups. The genetic diversity in regenerating juveniles of all species was as high as in putatively maternal stands. The detection of adequate (substantial) genetic diversity in the studied regenerating populations of these five broadleaved tree species suggests that in Lithuania these species have a good potential to adapt to changing environmental conditions.
Carbon Fiber-Reinforced Geopolymer Composites: A Review
Authors: Růžek Vojtěch , Dostayeva Ardak Mukhamedievna , Walter Janusz , Grab Thomas , Korniejenko Kinga
Published: February 2023

The article summarizes the state of the art in carbon-reinforced geopolymers. It takes into consideration various types of matrices and types of carbon fibers (CFs). The article shows the growing importance of this composite in the investigation conducted in recent years. Today, it is one of the most promising modern research areas, taking into account the decrease in the prices of CFs and their appearance on the market waste-based CFs, as well as research on new methods of producing CFs from sustainable precursors. The research methods applied in the article are critical analyses of the literature. The results of the literature analysis are discussed in a comparative context, including production methods and the influence of CFs on geopolymer properties. The potential applications for carbon fiber-reinforced geopolymer composites are shown. Additionally, the current research challenges for geopolymer composites reinforced by CFs are presented.
β-Lactamase Producing Escherichia coli Encoding blaCTX-M and blaCMY Genes in Chicken Carcasses from Egypt
Authors: Abo-Almagd Elham Elsayed , Sabala Rana Fahmi , Abd-Elghany Samir Mohammed , Jackson Charlene R. , Ramadan Hazem , Imre Kálmán , Morar Adriana , Herman Viorel , Sallam Khalid Ibrahim
Published: February 2023

Escherichia coli with multidrug resistance and β-lactamase genes may constitute a great public health hazard due to the potential for their transmission to humans through the food chain. This study determined the prevalence, antibiotic resistance profiles, phylogroups, and β-lactamase genes of E. coli isolates from chicken carcasses marketed in Mansoura, Egypt. Interestingly, E. coli was detected in 98% (98/100) of the chicken carcasses examined, which seemed among the highest contamination rates by E. coli worldwide. From the 425 genetically verified uidA gene-positive E. coli, 85 isolates were further studied for antimicrobial resistance profiles, phylogroups, and β-lactamase genes. Interestingly, 89.41% of E. coli (76/85) strains tested against 24 different antibiotics were multidrug-resistant. Of the examined 85 E. coli isolates, 22 (25.88%) isolates harbored blaCTX-M and were resistant to ampicillin, cefazoline, and ceftriaxone, while three of them were resistant to ceftazidime besides. Nine (10.59%) E. coli strains harbored AmpC- β-lactamase blaCMY and were resistant to ampicillin. One isolate co-carried blaCMY and blaCTX-M genes, though it was negative for the blaTEM gene. Of the 35 isolates that harbored either extended-spectrum β-lactamase (ESBL) and/or AmpC β-lactamase genes, six strains (17.14%) were assigned to pathogenic phylogroup F and one to phylogroup E, whereas 28 (80%) isolates belonged to commensal phylogenetic groups.
Characterization of a Galactose Oxidase from Fusarium odoratissimum and Its Application in the Modification of Agarose
Authors: Cao Na , Xia Guangli , Sun Huihui , Zhao Ling , Cao Rong , Jiang Hong , Mao Xiangzhao , Liu Qi
Published: February 2023

A galactose oxidase gene, gao-5f, was cloned from Fusarium odoratissimum and successfully expressed in E. coli. The galactose oxidase GAO-5F belongs to the AA5 family and consists of 681 amino acids, with an estimated molecular weight of 72 kDa. GAO-5F exhibited maximum activity at 40 °C and pH 7.0 and showed no change in activity after 24 h incubation at 30 °C. Moreover, GAO-5F exhibited 40% of its maximum activity after 24 h incubation at 50 °C and 60% after 40 h incubation at pH 7.0. The measured thermostability of GAO-5F is superior to galactose oxidase’s reported thermostability. The enzyme exhibited strict substrate specificity toward D-galactose and oligosaccharides/polysaccharides containing D-galactose. Further analysis demonstrated that GAO-5F specifically oxidized agarose to a polyaldehyde-based polymer, which could be used as a polyaldehyde to crosslink with gelatin to form edible packaging films. To our knowledge, this is the first report about the modification of agarose by galactose oxidase, and this result has laid a foundation for the further development of edible membranes using agarose.
Effects of W/O Nanoemulsion on Improving the Color Tone of Beijing Roast Duck
Authors: Teng Wendi , Yao Xinshuo , Li Jingyi , Wang Jinpeng , Cao Jinxuan
Published: February 2023

Traditional Beijing roast duck is often brushed with a high concentration of maltose solution (15% w/v) and shows ununiform color after roasting. A novel W/O nanoemulsion was applied to improve the color tone of Beijing roast ducks and, meanwhile, reduced the amount of sugar. For the W/O emulsion, 3% (w/v) xylose solution as the aqueous phase, soybean oil as the oil phase, and polyglycerol polyricinoleate (PGPR) and whey protein isolate (WPI) as co-emulsifiers were fabricated by high-pressure homogenization. Particle size measurement by Zetasizer and stability analysis by Turbiscan stability analyzer showed that WPI as co-emulsifier and internal aqueous phase at pH 9 decreased the droplet size and improved the emulsion stability. In addition, by color difference evaluation, the W/O nanoemulsion improved the Maillard reaction degree and color tone of Beijing roast duck. The molecular structure and key composition of pigments on the surface of Beijing roast duck skins were also identified and characterized by UV–vis spectroscopy and UHPLC-MS. This study creatively offers theoretical guidance for increasing applications of W/O-nanoemulsion-based Maillard reaction in the roast food industry, especially for the development of reduced-sugar Beijing roast duck with uniform and desired color satisfying consumers’ acceptance and marketability.
An Accurate, Rapid and Cost-Effective Method for T-nos Detection Based on CRISPR/Cas12a
Authors: Wang Yuling , Peng Cheng , Ding Lin , Su Zhixun , Chen Xiaoyun , Wang Xiaofu , Sun Meihao , Xu Junfeng
Published: February 2023

CRISPR/Cas12a technology is used for nucleic acid detection due to its specific recognition function and non-specific single-stranded DNA cleavage activity. Here, we developed a fluorescence visualisation detection method based on PCR and CRISPR/Cas12a approaches. The method was used to detect the nopaline synthase terminator (T-nos) of genetically modified (GM) crops, circumventing the need for expensive instruments and technicians. For enhanced sensitivity and stability of PCR-CRISPR/Cas12a detection, we separately optimised the reaction systems for PCR amplification and CRISPR/Cas12a detection. Eleven samples of soybean samples were assessed to determine the applicability of the PCR-CRISPR/Cas12a method. The method could specifically detect target gene levels as low as 60 copies in the reaction within 50 min. In addition, accurate detection of all 11 samples confirmed the applicability. The method is not limited by large-scale instruments, making it suitable for mass detection of transgenic components in plants in the field. In conclusion, we developed a new, accurate, rapid, and cost-effective method for GM detection.
New Synthetic Operon Vectors for Expressing Multiple Proteins in the Chlamydomonas reinhardtii Chloroplast
Authors: Yeon Jihye , Miller Stephen M. , Dejtisakdi Wipawee
Published: February 2023

Microalgae are a promising platform for generating valuable commercial products, including proteins that may not express well in more traditional cell culture systems. In the model green alga Chlamydomonas reinhardtii, transgenic proteins can be expressed from either the nuclear or chloroplast genome. Expression in the chloroplast has several advantages, but technology is not yet well developed for expressing multiple transgenic proteins simultaneously. Here, we developed new synthetic operon vectors to express multiple proteins from a single chloroplast transcription unit. We modified an existing chloroplast expression vector to contain intercistronic elements derived from cyanobacterial and tobacco operons and tested the ability of the resulting operon vectors to express two or three different proteins at a time. All operons containing two of the coding sequences (for C. reinhardtii FBP1 and atpB) expressed the products of those genes, but operons containing the other two coding sequences (C. reinhardtii FBA1 and the synthetic camelid antibody gene VHH) did not. These results expand the repertoire of intercistronic spacers that can function in the C. reinhardtii chloroplast, but they also suggest that some coding sequences do not function well in the context of synthetic operons in this alga.
Identification and Molecular Characterization of RWP-RK Transcription Factors in Soybean
Authors: Amin Nooral , Ahmad Naveed , Khalifa Mohamed A. S. , Du Yeyao , Mandozai Ajmal , Khattak Aimal Nawaz , Piwu Wang
Published: February 2023

The RWP-RK is a small family of plant-specific transcription factors that are mainly involved in nitrate starvation responses, gametogenesis, and root nodulation. To date, the molecular mechanisms underpinning nitrate-regulated gene expression in many plant species have been extensively studied. However, the regulation of nodulation-specific NIN proteins during nodulation and rhizobial infection under nitrogen starvation in soybean still remain unclear. Here, we investigated the genome-wide identification of RWP-RK transcription factors and their essential role in nitrate-inducible and stress-responsive gene expression in soybean. In total, 28 RWP-RK genes were identified from the soybean genome, which were unevenly distributed on 20 chromosomes from 5 distinct groups during phylogeny classification. The conserved topology of RWP-RK protein motifs, cis-acting elements, and functional annotation has led to their potential as key regulators during plant growth, development, and diverse stress responses. The RNA-seq data revealed that the up-regulation of GmRWP-RK genes in the nodules indicated that these genes might play crucial roles during root nodulation in soybean. Furthermore, qRT-PCR analysis revealed that most GmRWP-RK genes under Phytophthora sojae infection and diverse environmental conditions (such as heat, nitrogen, and salt) were significantly induced, thus opening a new window of possibilities into their regulatory roles in adaptation mechanisms that allow soybean to tolerate biotic and abiotic stress. In addition, the dual luciferase assay indicated that GmRWP-RK1 and GmRWP-RK2 efficiently bind to the promoters of GmYUC2, GmSPL9, and GmNIN, highlighting their possible involvement in nodule formation. Together, our findings provide novel insights into the functional role of the RWP-RK family during defense responses and root nodulation in soybean.
Craniofacial Development Is Fine-Tuned by Sox2
Authors: Mandalos Nikolaos Panagiotis , Dimou Aikaterini , Gavala Maria Angeliki , Lambraki Efstathia , Remboutsika Eumorphia
Published: February 2023

The precise control of neural crest stem cell delamination, migration and differentiation ensures proper craniofacial and head development. Sox2 shapes the ontogeny of the cranial neural crest to ensure precision of the cell flow in the developing head. Here, we review how Sox2 orchestrates signals that control these complex developmental processes.
The Lower Cretaceous “Vigla” Shales Potentiality to Be Source Rocks in the Ionian Basin, Greece
Authors: Bourli Nicolina , Pasadakis Nikolaos , Chamilaki Eleni , Sianni Maria , Zelilidis Avraam
Published: February 2023

As Lower Cretaceous “Vigla” shales have been suggested as one of the main source rocks for the Ionian Basin in Greece, a geochemical analysis was performed for “Vigla” shales in Kastos Island and the Araxos peninsula, far from the already studied areas. Results, based on Rock-Eval VI analysis, sample fractionation, and biomarkers analysis, showed that the studied rocks could be of low production capacity, are type II/III of kerogen, and can produce liquid and gas hydrocarbons for Kastos Island. Organic matter (total organic carbon-TOC 0.02–3.45%) of the studied samples is thermally immature, in the early stages of diagenesis, and was accumulated in an anoxic environment. Additionally, the geochemical analyses confirmed the combination of marine and terrestrial origin of the organic matter. On the other hand, TOC (0.01–0.72%) from the Araxos peninsula shows fair oil potential and type IV kerogen. The results based on the Odd–Even Predominance, OEP (27–31), OEP (2), and OEP (1), valued for samples AG1, AG2, AG5, and AG6, indicated an anoxic deposition environment. As the Ionian Basin was sub-divided into three sub-basins (internal, middle, and external) during its syn-rift evolution, different depositional conditions were developed from one sub-basin to the other, with different sedimentary thicknesses within the same sub-basin or among different sub-basins and with different amounts of TOC. The fact that there is a great difference in geochemical indices between the two studied areas during the same period suggests that probable different depositional conditions could exist. It seems that the richness in Kastos Island could be related to the neighboring Apulian Platform, whereas the poorness in the Araxos peninsula could be related to the Gavrovo platform, or the differences could be related to restrictions produced regions. The comparison with previous studies indicates that different quality and quantity of organic matter could be accumulated either within the same sub-basin or from one sub-basin to the other.
Defining Regional and Local Sediment Sources in the Ancestral Colorado River System: A Heavy Mineral Study of a Mixed Provenance Unit in the Fish Creek-Vallecito Basin, Southern California
Authors: McGill Paula , Nicholson Uisdean , Frei Dirk , Macdonald David
Published: February 2023

The Colorado River has flowed across the dextral strike-slip plate boundary between the North American and Pacific plates since the latest Miocene or earliest Pliocene. The Fish Creek-Vallecito Basin (FCVB) lies on the Pacific Plate in southern California, dextrally offset from the point where the modern Colorado river enters the Salton Trough; it contains a record of ancestral Colorado River sedimentation from 5.3–2.5 Ma. The basin stratigraphy exhibits a changing balance between locally derived (L-Suite) and Colorado River (C-Suite) sediments. This paper focuses on the Palm Springs Group (PSG), a thick fluvial and alluvial sequence deposited on the upper delta plain (between 4.2–2.5 Ma) when the Colorado was active in the area, allowing the detailed examination of the processes of sediment mixing from two distinct provenance areas. The PSG consists of three coeval formations: 1) Canebrake Conglomerate, a basin margin that has coarse alluvial fan deposits derived from surrounding igneous basement; 2) Olla Formation, fan-fringe sandstones containing L-Suite, C-Suite, and mixed units; and 3) Arroyo Diablo Formation, mineralogically mature C-Suite sandstones. Stratigraphic analysis demonstrates that the river flowed through a landscape with relief up to 2000 m. Satellite mapping and detailed logging reveal a variable balance between the two suites in the Olla Formation with an apparent upward increase in L-Suite units before abrupt cessation of Colorado sedimentation in the basin. Stable heavy mineral indices differentiate L-Suite (high rutile:zircon index: RZi 40–95) from C-Suite (RZi: 0–20). Both suites have garnet:zircon index (GZi) and apatite:tourmaline index (ATi) mostly above 50, although many L-suite and mixed Olla samples have much lower ATi (20–50), suggesting that the distal floodplain was wet and the local sediment had a longer residence time there, or went through several cycles of erosion and redeposition. Heavy mineral analysis, garnet geochemical analysis, and detrital zircon U-Pb age spectra allow us to quantify the amount of mixing from different sediment sources. These data show that about 30% of the mixed units are derived from the Colorado River and that up to 20% of the L-Suite is also derived from the Colorado River, suggesting that there was mutual cannibalisation of older deposits by fluvial channels in a transitional area at the basin margin. Although this study is local in scope, it provides an insight into the extent and nature of sediment mixing in a two-source system. We conclude that most ‘mixing’ is actually interbedding from separate sources; true mixing is facilitated by low subsidence rates and the rapid migration of fluvial channels.
Assessment of the Response of Trenched–Backfilled Pipelines to Strike-Slip Faults: An Analytical Approach
Authors: Asgarihajifirouz Mozhgan , Dong Xiaoyu , Shiri Hodjat
Published: February 2023

Trenched pipelines may experience significant lateral displacement due to natural geohazards such as strike slip-fault movements, landslides, etc. Using pre-excavated soil to backfill trenches is a cost-effective option to protect pipelines against large deformations. These backfilling materials are heavily remolded and therefore softer than the native ground. Therefore, the shear strength difference between the backfill and native ground may affect the pipeline–backfill–trench interaction and the failure mechanism of the surrounding soil. By assuming a simplified uniform soil domain, the influence of softer pre-excavated backfilling material on the pipeline–backfill–trench interaction is neglected in the analytical methods that are usually used in the structural health monitoring of buried pipelines. In this study, the effects of trenching and backfilling were incorporated into an analytical solution for a fast assessment of the pipeline response at the early stages of engineering design projects and structural health monitoring. In comparison with other methods, this methodology provides a convenient and efficient method for computing pipeline strain and deflection curves in geohazardous regions.
A Novel Physical Mobility Task to Assess Freezers in Parkinson’s Disease
Authors: Nóbrega Lígia Reis , Rocon Eduardo , Pereira Adriano Alves , Andrade Adriano de Oliveira
Published: February 2023

Freezing of gait (FOG), one of the most disabling features of Parkinson’s disease (PD), is a brief episodic absence or marked reduction in stride progression despite the intention to walk. Progressively more people who experience FOG restrict their walking and reduce their level of physical activity. The purpose of this study is to develop and validate a physical mobility task that induces freezing of gait in a controlled environment, employing known triggers of FOG episodes according to the literature. To validate the physical mobility tasks, we recruited 10 volunteers that suffered PD-associated freezing (60.6 ± 7.29 years-old) with new FOG-Q ranging from 12 to 26. The validation of the proposed method was carried out using inertial sensors and video recordings. All subjects were assessed during the OFF and ON medication states. The total number of FOG occurrences during data collection was 144. The proposed tasks were able to trigger 120 FOG episodes, while the TUG test caused 24. The Inertial Measurement Unit (IMU) with accelerometer and gyroscope could not only detect FOG episodes but also allowed us to visualize the three types of FOG: akinesia, festination and trembling in place.
Validating the German Short Basic Psychological Need Satisfaction and Frustration Scale in Individuals with Depression
Authors: Heissel Andreas , Sanchez Alba , Pietrek Anou , Bergau Theresa , Stielow Christiane , Rapp Michael A. , Van der Kaap-Deeder Jolene
Published: February 2023

Satisfaction and frustration of the needs for autonomy, competence, and relatedness, as assessed with the 24-item Basic Psychological Need Satisfaction and Frustration Scale (BPNSFS), have been found to be crucial indicators of individuals’ psychological health. To increase the usability of this scale within a clinical and health services research context, we aimed to validate a German short version (12 items) of this scale in individuals with depression including the examination of the relations from need frustration and need satisfaction to ill-being and quality of life (QOL). This cross-sectional study involved 344 adults diagnosed with depression (Mage (SD) = 47.5 years (11.1); 71.8% females). Confirmatory factor analyses indicated that the short version of the BPNSFS was not only reliable, but also fitted a six-factor structure (i.e., satisfaction/frustration X type of need). Subsequent structural equation modeling showed that need frustration related positively to indicators of ill-being and negatively to QOL. Surprisingly, need satisfaction did not predict differences in ill-being or QOL. The short form of the BPNSFS represents a practical instrument to measure need satisfaction and frustration in people with depression. Further, the results support recent evidence on the importance of especially need frustration in the prediction of psychopathology.
The Effectiveness of Day Hospitals in the Personal Recovery of Mental Disorder Patients during the COVID-19 Pandemic
Authors: Sánchez-Guarnido Antonio José , Ruiz-Granados María Isabel , Herruzo-Cabrera Javier , Herruzo-Pino Carlos
Published: February 2023

Background: In recent years, a new recovery model has gained ground in which recovery is understood as a process of change where individuals are able to improve their health and wellbeing, lead self-sufficient lives and strive to achieve their maximum potential (personal recovery). Despite the existence of data regarding the effectiveness of mental health day hospitals (MHDHs) in reducing relapses in terms of hospital admissions and emergencies, no studies have to date assessed how this change affected the new personal recovery model. Objectives: To verify the effectiveness of MHDHs in improving personal recovery processes among people with mental disorders (MDs). Methods: A prospective cohort study. A group of patients receiving follow-up at MHDHs was compared with another group of patients receiving follow-up in other therapeutic units over a period of three months. Results: Patient recovery at the MHDHs, assessed using the Individual Recovery Outcomes Counter (I.ROC), was found to be significantly better than that of patients attended in other units. Conclusions: MHDHs can contribute to the recovery of people with MDs. This is particularly important at a time when some patients may have experienced impediments to their recovery processes due to the pandemic.
Cytomorphometric and Clinical Changes in Gingival Tissue after Subgingival Tooth Preparation—A Pilot Study
Authors: Jovanovic Marija , Zivkovic Nikola , Gligorijevic Nikola , Igic Marko , Petrovic Milica , Bojovic Marija , Jovanovic Rodoljub , Kostic Milena
Published: February 2023

Tooth preparation for a metal-ceramic crown with a subgingival finish line can lead to inflammatory changes in the gingival tissue, often accompanied by cell damage. This study aimed to evaluate the clinical signs of inflammation and the cytomorphological status of the gingival tissue before and after tooth preparation. The research included a homogeneous group of 19 patients with an indication for upper canine preparation. Before and after treatment, the gingival and the gingival bleeding indexes were determined, gingival swabs were taken, and direct smears prepared on slides for cytomorphometric analysis. The values of the measured gingival indexes were statistically significantly higher (p < 0.001) after tooth preparation. They decreased over time, which indicated the reversibility of the resulting changes. Cytological examination showed no statistically significant difference between the values of nuclear area, perimeter, Feret diameter, Feret angle, integrated optical density, MinFeret, and roundness, before and after the treatment. Significantly higher values of circularity, integrated optical density, MinFeret (p < 0.05), as well as roundness (p < 0.001) were found after 72 h, compared to those taken 15 min after tooth crown preparation. This study is a pioneering attempt to show gingival changes during fixed prosthodontic treatment and may shed new light on pathogenetic events in prosthodontic patients.
Artisanal Gem Mining in Brazil: A Source of Genotoxicity and Exposure to Toxic Elements
Authors: Santos Ana , Silva Lucas , Freire Bruna , da Silva Faria Márcia , Batista Bruno , Rocha Bruno , Barbosa Fernando , Rodrigues Jairo
Published: February 2023

Environmental and occupational exposure to toxic metals has led many people around the world to have serious health problems. Mining activities contribute to an increased risk of exposure to these elements. In this work, a study of environmental biomonitoring and routes of exposure to toxic metals in a region of artisanal mining was performed. This study was carried out in the district of Taquaral de Minas, located in the Jequitinhonha Valley in the state of Minas Gerais. The valley is one of the wealthiest and highest gem-producing areas in Brazil. Five artisanal mines were sampled (Bode, Pirineu, Pinheira, Lajedo, and Marmita). Several potentially toxic metals (Be, Zn, Mn, Ba Cd, Hg, and U) were investigated in the soils and dust over the rocks and the soils. Samples from 22 individuals occupationally exposed and 17 unexposed persons, who formed the reference group, were analyzed for trace elements by an inductively coupled plasma mass spectrometer. The genotoxicity was evaluated by the micronucleus test in buccal mucosa epithelial cells, where the following changes were scored: micronuclei (MN) binucleate (BN) cells and kariolytic (KL) cells. The MN test showed significantly increased frequencies in all alterations of exposed individuals compared to the controls (p < 0.05, Student’s t-test). The urine analysis showed levels of Cr, Ni Ba, Pb, and As in the blood, which were higher than the ATSDR recommended levels. The association between the MN test and the trace element concentrations found in the blood and urine was significant (p < 0.05). The higher the number of years of working, the higher the concentrations in the blood were, due to chronic exposure. The results of the present study indicate environmental contamination and a potential risk to the health of miners, suggesting an intervention.
Cost Sharing and Cost Shifting Mechanisms under a per Diem Payment System in a County of China
Authors: Liu Fengrong , Chen Jiayu , Li Chaozhu , Xu Fenghui
Published: February 2023

Cost sharing and cost shifting mechanisms are of vital importance in a prospective payment system. This paper employed the difference-in-differences method to estimate the impacts of a per diem system with inverted-U-shape rates on medical costs and the length of stay based on data from a health insurance institution. The supply side cost sharing mechanism worked so that the new payment system significantly reduced medical costs by 17.59 percent while the average length of stay varied little. After further analyzing the mechanism, we found that heterogeneous effects emerged mainly due to the special rates design. The reform decreased the cases that incurred relatively high medical costs and lengths of stay. However, cost shifting existed so that physicians could be motivated to provide unnecessary services to the patients who should have been discharged before the average length of stay. Therefore, payment rates in the per diem system require a sophisticated design to constrain its distortion to medical service provision even though medical expenditures were successfully contained.
Preventive Behaviors and Influencing Factors among Thai Residents in Endemic Areas during the Highest Epidemic Peak of the COVID-19 Outbreak
Authors: Ounsaneha Weerawat , Laosee Orapin , Suksaroj Thunwadee Tachapattaworakul , Rattanapan Cheerawit
Published: February 2023

This research aims to investigate COVID-19 preventive behavior and influencing factors among Thai residents during the highest epidemic peak of COVID-19. Nine hundred and forty-six residents in five districts with high COVID-19 infection cases in Thailand were systematically included in this cross-sectional survey. The results showed that 87.2% and 65.2% of the residents had a high level of general knowledge and preventive measures, respectively. As to COVID-19 attitudes, poor levels of attitude among Thai residents were found in risk perception (53.6%) and mistrust issues (70.4%). Moreover, this study presents good preventive behavior (77.0%) among Thai residents. Multiple logistic regression showed that the influence factors of COVID-19 preventive behavior were the young age group (AOR 2.97, 95% CI 1.68–5.25), high income (AOR 1.38, 95% CI 1.03–1.86), and high level of general COVID-19 knowledge (AOR 2.21, 95% CI 1.64–2.96). The conclusion was that providing information on COVID-19 via social media was the key mechanism of policy action for increasing the level of COVID-19 preventive behavior during the highest epidemic peak in Thailand. In addition, the pandemic preparedness and response policy, with resident participation and involvement, could be recommended for the resilience of pandemic preparedness.
Epidemiological Profile among Greek CrossFit Practitioners
Authors: Vassis Konstantinos , Siouras Athanasios , Kourkoulis Nikolaos , Poulis Ioannis A. , Meletiou Georgios , Iliopoulou Anna-Maria , Misiris Ioannis
Published: February 2023

CrossFit (CF) is a popular and rapidly expanding training program in Greece and worldwide. However, there is a lack of scientific evidence on the risk of musculoskeletal injuries related to CF in the Greek population. A self-administered survey of 1224 Greek CF practitioners aged 18 to 59 was conducted and analyzed using the Statistical Package for Social Sciences (SPSS) software. The highest percentage of the participants (34%) practiced 5 days per week for 60 min (42.2%) and had 2 days per week of rest (41.7%). A total of 273 individuals (23%) participated in CF competitions and 948 (77%) did not. The results showed that the most common injuries were muscle injuries (51.3%), followed by tendinopathies (49.6%) and joint injuries (26.6%). The shoulders (56.6%; n = 303), knees (31.8%; n = 170), and lumbar spine (33.1%; n = 177) were the most commonly injured locations. The logistic regression model showed that participation in competitions (p = 0.001), rest per week (p = 0.01), duration of training per session (p = 0.001), and frequency of training per week (p = 0.03) were statistically significant factors for injury. Training level was not a statistically significant factor for injury (p = 0.43). As CF continues to gain popularity on a global scale and the number of athletes gradually increases, it is important to monitor the safety of practitioners. Clinicians should consider participation in competitions, rest, training duration, and frequency in order to make CF safer.
Vital Needs of Dutch Homeless Service Users: Responsiveness of Local Services in the Light of Health Equity
Authors: van Everdingen Coline , Peerenboom Peter Bob , van der Velden Koos , Delespaul Philippe
Published: February 2023

Background: Healthcare and social services aim to ensure health equity for all users. Despite ongoing efforts, marginalized populations remain underserved. The Dutch HOP-TR study intends to expand knowledge on how to enable the recovery of homeless service users. Methods: A naturalistic meta-snowball sampling resulted in a representative sample of homeless services (N = 16) and users (N = 436). Interviews collected health and needs from user and professional perspectives in a comprehensive, rights-based ecosystem strategy. We calculated the responsiveness to needs in four domains (mental health, physical health, paid work, and administration). Results: Most service users were males (81%) with a migration background (52%). In addition to physical (78%) and mental health needs (95%), the low education level (89%) and functional illiteracy (57%) resulted in needs related to paid work and administration support. Most had vital needs in three or four domains (77%). The availability of matching care was extremely low. For users with needs in two domains, met needs ranged from 0.6–13.1%. Combined needs (>2 domains) were hardly met. Conclusions: Previous research demonstrated the interdependent character of health needs. This paper uncovers some causes of health inequity. The systematic failure of local services to meet integrating care needs demonstrates the urgency to expand recovery-oriented implementation strategies with health equity in mind.
Metabolic Syndrome-Related Knowledge, Attitudes, and Behavior among Indigenous Communities in Taiwan: A Cross-Sectional Study
Authors: Lo Shu-Fen , Lu Fang-Tsuang , O. Yang An-Chi , Zeng Jia-Ling , Yang Ya-Yu , Lo Yen-Ting , Chang Yu-Hsuan , Pai Ting-Hsuan
Published: February 2023

Background: Metabolic syndrome is characterized by cardiovascular and chronic disease risk factors that cause health problems. Inequalities in medical resources and information present a challenge in this context. Indigenous communities may be unaware of their risk for metabolic syndrome. Aims: This study explored factors associated with metabolic syndrome-related knowledge, attitudes, and behaviors among Taiwanese indigenous communities. Methods: For this descriptive cross-sectional survey, we collected anthropometric data and used a self-administered questionnaire between 1 July 2016, to 31 July 2017, from a convenience sample of an indigenous tribe in eastern Taiwan. The response rate was 92%. Results: The prevalence of metabolic syndrome was as high as 71%, and the average correct knowledge rate was 39.1%. The participants’ self-management attitudes were mainly negative, and the self-management behaviors were low in this population. Stepwise regression analysis showed that knowledge, attitude, age, perception of physical condition, and body mass index, which accounted for 65% of the total variance, were the most predictive variables for self-management behaviors. Conclusions: This is the first study to report the relationship between metabolic syndrome knowledge, attitudes, and behaviors in an indigenous population. There is an urgent need to develop safety-based MetS health education programs that can provide access to the right information and enhance self-management approaches to lessen the growing burden of MetS in indigenous communities.
Economic Justification Analysis of Minimally Invasive versus Conventional Aortic Valve Replacement
Authors: Jovanovic Marko , Zivkovic Igor , Jovanovic Milos , Bilbija Ilija , Petrovic Masa , Markovic Jovan , Radovic Ivana , Dimitrijevic Ana , Soldatovic Ivan
Published: February 2023

There is no definitive consensus about the cost-effectiveness of minimally invasive aortic valve replacement (AVR) (MI-AVR) compared to conventional AVR (C-AVR). The aim of this study was to compare the rate of postoperative complications and total hospital costs of MI-AVR versus C-AVR overall and by the type of aortic prosthesis (biological or mechanical). Our single-center retrospective study included 324 patients over 18 years old who underwent elective isolated primary AVR with standard stented AV prosthesis at the Institute for Cardiovascular Diseases “Dedinje” between January 2019 and December 2019. Reintervention, emergencies, combined surgical interventions, and patients with sutureless valves were excluded. In both MI-AVR and C-AVR, mechanical valve implantation contributed to overall reduction of hospital costs with equal efficacy. The cost-effectiveness ratio indicated that C-AVR is cheaper and yielded a better clinical outcome with mechanical valve implantation (67.17 vs. 69.5). In biological valve implantation, MI-AVR was superior. MI-AVR patients had statistically significantly higher LVEF and a lower Euro SCORE than C-AVR patients (Mann–Whitney U-test, p = 0.002 and p = 0.002, respectively). There is a slight advantage to MI-AVR vs. C-AVR, since it costs EUR 9.44 more to address complications that may arise. Complications (mortality, early reoperation, cerebrovascular insult, pacemaker implantation, atrial fibrillation, AV block, systemic inflammatory response syndrome, wound infection) were less frequent in the MI-AVR, making MI-AVR more economically justified than C-AVR (18% vs. 22.1%).
Making Sense or Non-Sense? Communicating COVID-19 Guidelines to Young Adults at Danish Folk High Schools
Authors: Vildekilde Thilde , Connelly Julie Jakobsen , van Houten Charlotte Sophie , Hansen Jakob Thestrup , Sørensen Jane Brandt , Meyrowitsch Dan Wolf , Konradsen Flemming
Published: February 2023

Little is known about young people’s behaviors and responses under outbreaks of infectious diseases such as the COVID-19 pandemic, especially in institutional settings. This research investigated the reactions of young adults residing at Danish folk high schools (FHSs) towards COVID-19 guidelines and the communicative styles used to enforce COVID-19 guidelines. The qualitative data consists of focus group discussions (FGDs) with students, interviews with staff, and participant observations, as well as survey data from 1800 students. This study showed that young adults reacted negatively when first faced with the new reality of COVID-19 restrictions. They expressed distress over the loss of meaning (non-sense), loss of sense of community, as well as uncertainty. Hygiene guidelines, however, made immediate sense and were socially well accepted. Most FHSs actively involved students in risk communication and creative examples of community-building communication were identified. This study demonstrates that successful risk communication at educational institutions must take into consideration how young adults make sense of and cope with the uncertainties of life during crisis situations including epidemics.
Meals on Wheels: Promoting Food and Nutrition Security among Older Persons in Cape Town, South Africa
Authors: Nkurunziza Magnifique , Mchiza Zandile June-Rose , Zembe Yanga
Published: February 2023

Food insecurity (FI) prevails in Sub-Saharan Africa. Yet, in South Africa, although many people, including the elderly, are vulnerable to FI, little is known about the experiences of older persons (OPs) with FI and the interventions thereof. In South Africa, Meals on Wheels Community Service (MOWCS) provides readymade home meal deliveries for OPs through 209 branches across the country. Therefore, this study investigated MOWCS’ role in the promotion of food security among the OPs at the Brooklyn branch, Cape Town. The study was grounded within the food security framework and focused on the availability, accessibility, utilization, and stability of food at Brooklyn MOWCS. Using qualitative research methods, 10 semi-structured interviews and one focus group discussion (N = 5) were conducted with Brooklyn MOWCS beneficiaries, in addition to three key personnel interviews conducted with staff. Data were analysed using Open Code 4.03. The findings showed Brooklyn MOWCS as a stable source of affordable and nutritious meals to OPs. The portion size satisfied hunger; occasionally, one portion sufficed for two meals. Respondents admitted the meal ingredients represented various food groups and rated them as “healthy”. However, some financial challenges hindered the extension of MOWCS services to the wider community. For instance, they only had three paid employees and were overcrowded within church premises. Findings also showed race and gender disparity among respondents; 90% were White and 10% were of Mixed Ancestry, with no Black or Asian OPs represented, and only 10% were male. These outcomes are typical of the current ethnic profile of the overall Brooklyn MOWCS beneficiaries in SA. This calls, therefore, for such interventions to be extended to all South African demographic groups as an initiative to alleviate food and nutrition insecurity among all OPs.