bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy and safety of very early rehabilitation for acute ischemic stroke: a systematic review and meta-analysis
Authors: Lou Ying , Liu Zhongshuo , Ji Yingxiao , Cheng Jinming , Zhao Congying , Li Litao
Published: October 2024

Background

Early rehabilitation after acute ischemic stroke (AIS) contributes to functional recovery. However, the optimal time for starting rehabilitation remains a topic of ongoing investigation. This article aims to shed light on the safety and efficacy of very early rehabilitation (VER) initiated within 48 h of stroke onset.

Methods

A systematic search in PubMed, Embase, Cochrane Library, and Web of Science databases was conducted from inception to January 20, 2024. Relevant literature on VER in patients with AIS was reviewed and the data related to favorable and adverse clinical outcomes were collected for meta-analysis. Subgroup analysis was conducted at different time points, namely at discharge and at three and 12 months. Statistical analyses were performed with the help of the Meta Package in STATA Version 15.0.

Results

A total of 14 randomized controlled trial (RCT) studies and 3,039 participants were included in the analysis. VER demonstrated a significant association with mortality [risk ratio (RR) = 1.27, 95% confidence interval (CI) (1.00, 1.61)], ability of daily living [weighted mean difference (WMD) = 6.90, 95% CI (0.22, 13.57)], and limb motor function [WMD = 5.02, 95% CI (1.63, 8.40)]. However, no significant difference was observed between the VER group and the control group in adverse events [RR = 0.89, 95% CI (0.79, 1.01)], severity of stroke [WMD = 0.52, 95% CI (−0.04, 1.08)], degree of disability [RR = 1.06, 95% CI (0.93, 1.20)], or recovery of walking [RR = 0.98, 95% CI (0.94, 1.03)] after stroke. Subgroup analysis revealed that VER reduced the risk of adverse events in the late stage (at three and 12 months) [RR = 0.86, 95% CI (0.74, 0.99)] and degree of disability at 12 months [RR = 1.28, 95% CI (1.03, 1.60)], and improved daily living ability at 3 months [WMD = 4.26, 95% CI (0.17, 8.35)], while increasing severity of stroke during hospitalization [WMD = 0.81, 95% CI (0.01, 1.61)].

Conclusion

VER improves activities of daily living (ADLs) and lowers the incidence of long-term complications in stroke survivors. However, premature or overly intense rehabilitation may increase mortality in patients with AIS during the acute phase. PROSPERO registration number: CRD42024508180.

Systematic review registration

This systematic review was registered with PROSPERO (https://www.crd.york.ac.uk/PROSPERO/). PROSPERO registration number: CRD42024508180.
The benefits of photobiomodulation in animal models of multiple sclerosis: a systematic review and meta-analysis
Authors: Ahmed Zubair
Published: October 2024

Background

Photobiomodulation (PBM), using red- or near-infrared light, has been used to treat tendinopathies, nerve injuries, osteoarthritis and wounds and evaluated in experimental allergic encephalomyelitis (EAE). To date, only a few studies have been performed in EAE but surprisingly, a few clinical studies in humans have already been performed, despite the paucity of preclinical evidence.

Objective

Therefore, this study systematically reviewed the usefulness of PBM in ameliorating the clinical signs of EAE, a commonly used animal model of multiple sclerosis, and determine if there is enough evidence to warrant human studies.

Methods

PubMed, EMBASE and Web of Science were searched in July 2024 for studies relating to PBM and EAE without any language restrictions. Since only three studies have been published, all studies were included in the systematic review and data related to clinical signs of EAE was pooled together to conduct a meta-analysis. Non-homogenous data was also reported and thematically synthesized.

Results

A meta-analysis of the pooled data from the three included studies demonstrated a significant reduction of the clinical severity of EAE, with a mean reduction of 1.44, 95% CI (−2.45, −0.42), p = 0.006. PBM also significantly reduced other parameters such as infiltration of mononuclear cells, CNS demyelination, apoptosis markers and pro-inflammatory cytokines. However, there was an overall high risk of bias in all of the studies.

Conclusion

The meta-analysis supports the use of PBM to ameliorate the symptoms of EAE, but the paucity of studies and the high risk of bias in the included studies warrants further preclinical investigation before conducting human studies.
Effect of fatigue on intermuscular EMG-EMG coupling during bench press exercise at 60% 1RM workload in males
Authors: Wang Lejun , Tao Haifeng , Chen Qing , Qiao Minjie , Song Xiaoqian , Niu Wenxin
Published: October 2024

Objective

To explore the neuromuscular control mechanism and quantifying the fatigue response during bench press exercise is important references to prescribe an appropriate exercise program. However, current literature struggles to provide a concrete conclusion on the changes of intermuscular EMG-EMG coupling between synergistic and antagonist muscles during the exercise. Thus, the current study was designed to reveal fatigue-related changes of intermuscular EMG-EMG coupling during bench press exercise.

Methods

Thirty-one healthy male participants performed a bench press exercise on the Smith machine at 60% One Repetition Maximum (1RM) workload to exhaustion, while surface electromyographic signals (sEMG) were collected from triceps brachii (TB), biceps brachii (BB), anterior deltoid (AD), posterior deltoid (PD), and pectoralis major (PM). Surface EMG signals were divided into the first half and second half of the bench press exercise. Phase synchronization index (PSI) was calculated between sEMG of synergistic muscle pairs AD-TB, AD-PM and antagonist muscle pairs BB-TB, AD-PD.

Results

EMG power of TB, AD, PD, PM muscles in alpha (8–12 Hz) frequency band and EMG power of each muscle in beta (15–35 Hz), and gamma (35–60 Hz) frequency bands were all increased during the second half of contraction compared with the first half of contraction. PSI of gamma frequency band was significantly decreased in BB-TB muscle pair while EMG-EMG coupling of AD-TB in gamma frequency band was significantly increased during the second half of contraction compared to the first half of contraction.

Conclusions

The results indicated a decrease of interconnection between synchronized cortical neurons and the motoneuron pool of BB and TB, and an increase of interconnection between AD-TB muscles during fatiguing bench press exercise at 60% 1RM workload. The changes of intermuscular coupling may be related to the supraspinal modulations to compensate for the decrease of muscle force as well as a result of unbalanced changes of agonist and antagonist muscle contractility.
Modulation of gut microbiome in response to the combination of Escherichia coli Nissle 1917 and sugars: a pilot study using host-free system reflecting impact on interpersonal microbiome
Authors: Heer Kiran , Kaur Manpreet , Sidhu Dwinder , Dey Priyankar , Raychaudhuri Saumya
Published: October 2024

Introduction

The differential effects of probiotic, prebiotic, and synbiotic formulations on human health are dictated by the inter-individual gut microbial profile. The effects of probiotics such as Escherichia coli Nissle 1917 (ECN) on gut microbiota may vary according to the microbiome profiles of individuals and may be influenced by the presence of certain carbohydrates, which can impact microbial community structure and treatment results.

Method

Processed fecal samples from donors having contrasting lifestyles, dietary patterns, and disease histories were mixed with 5 × 106 CFU/mL ECN with or without 1% (w/v) sugars (glucose, galactose, or rice starch) in a host-free system. Post-incubation, 16 s rRNA sequencing was performed. Microbial diversity and taxonomic abundance were computed in relation to the probiotic, prebiotic, and synbiotic treatment effects and interpersonal microbiome variance.

Result

Baseline gut microbial profiles showed significant inter-individual variations. ECN treatment alone had a limited impact on the inter-personal gut microbial diversity and abundance. Prebiotics caused a substantial enrichment in Actinobacteria, but there were differences in the responses at the order and genus levels, with enrichment shown in Bifidobacterium, Collinsella, and Megasphaera. Subject B exhibited enrichment in Proteobacteria and Cyanobacteria, but subject A showed more diversified taxonomic alterations as a consequence of the synbiotic treatments. Despite negligible difference in the α-diversity, probiotic, prebiotic, and synbiotic treatments independently resulted in distinct segregation in microbial communities at the β-diversity level. The core microbiota was altered only under prebiotic and synbiotic treatment. Significant correlations primarily for minor phyla were identified under prebiotic and synbiotic treatment.

Conclusion

The interindividual microbiome composition strongly influences the effectiveness of personalized diet and treatment plans. The responsiveness to dietary strategies varies according to individual microbiome profiles influenced by health, diet, and lifestyle. Therefore, tailored approaches that consider individual microbiome compositions are crucial for maximizing gut health and treatment results.
The pros and cons of probiotic use in pediatric oncology patients following treatment for acute lymphoblastic leukemia
Authors: Šimiaková Miroslava , Bielik Viktor
Published: October 2024

Acute lymphoblastic leukemia (ALL) treatment, involving chemotherapy, radiotherapy, and pharmacotherapy (antibiotics, antineoplastics) perturbs the gut microbiota in pediatric patients, with enduring effects post-treatment. ALL treatments diminish microbial richness and diversity, favoring pathogenic bacteria. Probiotics may offer promise in mitigating these disruptions and associated side effects. This mini-review explores the impact of ALL treatment on the gut microbiota and the potential benefits of probiotics in pediatric oncology. Probiotics have shown promise in restoring gut microbial balance, reducing treatment-associated side effects, and potentially improving quality of life. However, potential adverse effects, particularly in immunocompromised patients, warrant caution. Notably, there's emerging interest in probiotics’ role in bone health and mineral bioaccessibility. Further research is needed to elucidate probiotics’ mechanisms and their broader impact on pediatric health. Integration of probiotics into ALL treatment and post-treatment regimens offers significant potential for improving patient outcomes and reducing treatment-related complications and long-lasting disruptions, although careful monitoring is essential.
Performance of game sessions in VR vs standard 2D monitor environment. an EEG study
Authors: Malinowska Urszula , Wojciechowski Jakub , Waligóra Marek , Rogala Jacek
Published: October 2024

Nowadays studies using Virtual Reality (VR) are gaining high popularity due to VR being a better approximation of the ecological environment for visual experiments than standard 2D display settings. VR technology has been already applied in medicine in the therapy of mental disorders, neurorehabilitation, and neurofeedback. However, its effectiveness compared to the standard 2D procedure is still not fully documented and limited information about the neurophysiological underpinnings of VR is provided. In this study, we tested participants’ performance during several sessions of the computer game in two different environments, VR vs. 2D monitor display. Participants performed three 25 min gaming sessions of adapted Delay Match-To-Sample task during EEG recording. The results showed that the VR group outperformed the 2D display group in the first session and then maintained its performance level throughout the remaining two sessions while the 2D group increased performance in each session eventually leveling up in the last one. Also group differences in the EEG activity were most profound only in the first session. In this session, the VR group was characterized by stronger and more synchronized neuronal activity, especially in delta, theta, and gamma bands. The VR group was less impacted by visual arousals as indicated by the theta/beta2 ratio in parietal electrodes.
LT-DeepLab: an improved DeepLabV3+ cross-scale segmentation algorithm for Zanthoxylum bungeanum Maxim leaf-trunk diseases in real-world environments
Authors: Yang Tao , Wei Jingjing , Xiao Yongjun , Wang Shuyang , Tan Jingxuan , Niu Yupeng , Duan Xuliang , Pan Fei , Pu Haibo
Published: October 2024

Introduction

Zanthoxylum bungeanum Maxim is an economically significant crop in Asia, but large-scale cultivation is often threatened by frequent diseases, leading to significant yield declines. Deep learning-based methods for crop disease recognition have emerged as a vital research area in agriculture.

Methods

This paper presents a novel model, LT-DeepLab, for the semantic segmentation of leaf spot (folium macula), rust, frost damage (gelu damnum), and diseased leaves and trunks in complex field environments. The proposed model enhances DeepLabV3+ with an innovative Fission Depth Separable with CRCC Atrous Spatial Pyramid Pooling module, which reduces the structural parameters of Atrous Spatial Pyramid Pooling module and improves cross-scale extraction capability. Incorporating Criss-Cross Attention with the Convolutional Block Attention Module provides a complementary boost to channel feature extraction. Additionally, deformable convolution enhances low-dimensional features, and a Fully Convolutional Network auxiliary header is integrated to optimize the network and enhance model accuracy without increasing parameter count.

Results

LT-DeepLab improves the mean Intersection over Union (mIoU) by 3.59%, the mean Pixel Accuracy (mPA) by 2.16%, and the Overall Accuracy (OA) by 0.94% compared to the baseline DeepLabV3+. It also reduces computational demands by 11.11% and decreases the parameter count by 16.82%.

Discussion

These results indicate that LT-DeepLab demonstrates excellent disease segmentation capabilities in complex field environments while maintaining high computational efficiency, offering a promising solution for improving crop disease management efficiency.
Partial substitution of phosphorus fertilizer with iron-modified biochar improves root morphology and yield of peanut under film mulching
Authors: Luo Xiulan , Wang Dewei , Liu Yuting , Qiu Yuanze , Zheng Junlin , Xia Guimin , Elbeltagi Ahmed , Chi Daocai
Published: October 2024

Introduction

Peanut production is being increasingly threatened by water stress with the context of global climate change. Film mulching have been reported to alleviate the adverse impact of drought on peanut. Lower phosphorus use efficiency is another key factor limiting peanut yield. Application of iron-modified and phosphorus-loaded biochar (BIP) has been validated to enhance phosphorus utilization efficiency in crops. However, whether combined effect of film mulching and BIP could increase water use efficiency and enhance peanut production through regulating soil properties and root morphologies needs further investigation.

Methods

A two-year (2021-2022) pot experiment using a split-plot design was conducted to investigate the effects of phosphorus fertilizer substitution using BIP on soil properties, root morphology, pod yield, and water use of peanut under film mulching. The main plots were two mulching methods, including no mulching (M0) and film mulching (M1). The subplots were four combined applications of phosphorus fertilizer with BIP, including conventional phosphorus fertilizer rates (PCR) without BIP, P1C0; 3/4 PCR with 7.5 t ha-1 BIP, P2C1; 3/4 PCR with 15 t ha-1 BIP, P2C2; 2/3 PCR with 7.5 t ha-1 BIP, P3C1; 2/3 PCR with 15 t ha-1 BIP, P3C2.

Results and discussion

The results indicated that regardless of biochar amendments, compared with M0, M1 increased soil organic matter and root morphology of peanut at different growth stages in both years. In addition, M1 increased peanut yield and water use efficiency (WUE) by 18.8% and 51.6%, respectively, but decreased water consumption by 25.0%, compared to M0 (two-year average). Irrespective of film mulching, P2C1 increased length, surface area, and volume of peanut root at seedling by 16.7%, 17.7%, and 18.6%, at flowering by 6.6%, 19.9%, and 29.5%, at pod setting by 22.9%, 33.8%, and 37.3%, and at pod filling by 48.3%, 9.5%, and 38.2%, respectively (two-year average), increased soil pH and organic matter content during peanut growing season, and increased soil CEC at harvest. In general, the M1P2C1 treatment obtained the optimal root morphology, soil chemical properties, WUE, and peanut yield, which increased peanut yield by 33.2% compared to M0P1C0. In conclusion, the combination of film mulching with 7.5 t ha-1 BIP (M1P2C1) effectively improved soil chemical properties, enhanced root morphology of peanut, and ultimately increased peanut yield and WUE.
Environmental impacts and nitrogen-carbon-energy nexus of vegetable production in subtropical plateau lake basins
Authors: He Yousheng , Su Ruifeng , Wang Yuan , Li Shunjin , Huang Qi , Chen Xinping , Zhang Wei , Yao Zhi
Published: October 2024

Vegetables are important economic crops globally, and their production has approximately doubled over the past 20 years. Globally, vegetables account for 13% of the harvested area but consume 25% of the fertilizer, leading to serious environmental impacts. However, the quantitative evaluation of vegetable production systems in subtropical plateau lake basins and the establishment of optimal management practices to further reduce environmental risks are still lacking. Using the life cycle assessment method, this study quantified the global warming, eutrophication, acidification, and energy depletion potential of vegetable production in a subtropical plateau lake basin in China based on data from 183 farmer surveys. Our results indicated that vegetable production in the study area, the Erhai Lake Basin, was high but came at a high environmental cost, mainly due to low fertilizer efficiency and high nutrient loss. Root vegetables have relatively high environmental costs due to the significant environmental impacts of fertilizer production, transportation, and application. A comprehensive analysis showed that the vegetable production in this region exhibited low economic and net ecosystem economic benefits, with ranges of 7.88–8.91 × 103 and 7.35–8.69 × 103 $ ha−1, respectively. Scenario analysis showed that adopting strategies that comprehensively consider soil, crop, and nutrient conditions for vegetable production can reduce environmental costs (with reductions in global warming potential (GWP), eutrophication potential (EP), acidification potential (AP), and energy depletion potential (EDP) by 10.6–28.2%, 65.1–73.5%, 64.5–71.9%, 47.8–70.4%, respectively) compared with the current practices of farmers. This study highlighted the importance of optimizing nutrient management in vegetable production based on farmers’ practices, which can achieve more yield with less environmental impacts and thereby avoid the “trade-off” effect between productivity and environmental sustainability.
Gender differences in operational and cognitive abilities
Authors: Lager Emil , Sorjonen Kimmo , Melin Marika
Published: October 2024

Introduction

Gender differences in cognitive and operational abilities have been identified. Yet, their interrelationship remains underexplored. This prevents tailored evidence-based selection, allowing discrimination to persist.

Methods

Data from a test battery of operational and cognitive tests was analyzed. In total 2,743 aviation pilot candidates’ test scores were analyzed.

Results

Males had a significantly higher score on mental spatial ability, memory retention, abstract problem solving, multitasking ability (MU), and manual spatial ability (MSA); and females on perceptual speed. Correlations between MU and MSA [difference = 0.269 (95% CI: 0.114; 0.405)] and between MSA and perceptual speed [difference = 0.186 (95% CI: 0.027; 0.332)] were significantly stronger among female applicants. A high MSA score was more predictive of a high score on MU, Perceptual speed, and Memory for female compared with male applicants (p < 0.002 for the MSA score × sex interaction effect in all three cases).

Discussion

Interpretation of test scores in between genders potentially may need to look different for final selection decisions for operational professions, as female test profiles were shown to exhibit greater homogeneity.
Development of gross motor skills in children under the age of 3 years: a decision tree approach
Authors: Xiong Yuxiang , Hu Xuhuai , Cao Jindan , Shang Li , Yao Yibei , Niu Ben
Published: October 2024

Background

The early years of life are critical for gross motor development (GMD). This study utilized decision tree modeling to examine the influences on gross motor development in children under the age of 3 years and to rank the key factors affecting their development.

Methods

Based on randomized stratified sampling, 9,507 children aged 0–3 years in Shenzhen were included in this study. The Ages and Stages Questionnaires (ASQ) were utilized for the assessment of gross motor development. The chi-square test was used to compare groups, and variables were screened using univariable and multivariable regression analyses. Decision tree modeling was employed to rank the importance of statistically significant variables.

Results

The research found a prevalence of gross motor developmental delay of 1.41% among the respondents. The accuracy of the decision tree model is 70.96%. The results demonstrated a strong correlation between seven variables affecting the gross motor development of children, which were ranked based on importance: age, whether to provide supplementary food, average time spent interacting with children, family type, feeding method, mode of delivery, and birth order.

Conclusion

The risk of gross motor developmental delay increases with age. Furthermore, supplementary food and interacting with other children are critical factors in improving children’s GMD delay. It is therefore imperative to enhance the monitoring of children’s gross motor skills through regular developmental assessments that detect potential GMD delays. Moreover, family type, feeding method, mode of delivery, and birth order were also predictive factors of GMD delay.
Food consumption markers and sociodemographic factors associated with obesity among adult women registered in the Food and Nutrition Monitoring System (SISVAN) in Brazil: a cohort study, 2015–2019
Authors: Barros Anael Queirós Silva , Aguiar Italo Wesley Oliveira , Adriano Lia Silveira , Carioca Antônio Augusto Ferreira , Marchioni Dirce Maria Lobo , Sampaio Helena Alves de Carvalho
Published: October 2024

Introduction

Carrying out studies using data from Food and Nutritional Monitoring is crucial given the current epidemiological scenario. This scenario reveals a coexistence of malnutrition and nutritional deficiencies alongside overweight and obesity, particularly among adult women in Brazil. Analyzing the microdata generated by the system is essential for accurately representing food and nutrition indicators in the country.

Objective

To analyze the association of food consumption markers and sociodemographic factors with changes in the Body Mass Index (BMI) among adult women.

Methodology

A cohort study with a final sample of 30,354 women evaluated through individual records between the years 2015 and 2019. Nutritional status was assessed using body mass index (BMI). Food consumption markers used were the ones available in the system, which referred to foods consumed the previous day. Linear regression was used to evaluate factors associated with BMI and multiple Poisson regression was used to estimate the relative risk of obesity incidence.

Results

There was an increase in BMI between 2015 and 2019. The average increase in BMI was lower in women who consumed, in the previous day, beans (β = −0,25; CI95%: −0.36; −0.13) and vegetables and/or legumes (β = −0.12; CI95%: −0.21; −0.04) and the increase was greater when they consumed hamburgers and/or sausages (β = 0.1; CI95%: 0.02; 0.19) adjusted by age, region and skin color. There was a 14% lower incidence of obesity in the group that consumed beans (RR = 0.86; 95% CI: 0.79; 0.93), and a 9% lower incidence when they consumed filled cookies, candies or treats (RR = 0.91; 95% CI 0.86; 0.96). The incidence of obesity was 11% higher in the group that consumed sweetened drinks (RR = 1.11; 95% CI: 1.05; 1.18) and 9% higher in the group that consumed instant noodles, packaged snacks or savory biscuits on the day before the 2015 registration (RR = 1.09; 95% CI: 1.03; 1.15).

Conclusion

There was an increase in BMI in the population of adult women, rising nationally between 2015 and 2019, with the notable influence of diet on this change.
Exploring knowledge, perception, and use of surface electromyography in physiotherapy post graduate trainees in Italy: a single center preliminary survey
Authors: Bertoni Gianluca , Leuzzi Gaia , Job Mirko , De Simone Marica , Testa Marco
Published: October 2024

Introduction

Surface electromyography (sEMG) is a non-invasive technique that records muscle electrical activity using skin-surface electrodes, aiding physiotherapists in assessing and treating muscular and neuromuscular conditions. Despite its potential, sEMG remains underutilized in Italy. This study aims to evaluate Italian physiotherapists’ knowledge and use of sEMG, specifically among those who completed the Master's Degree in Rehabilitation of Musculoskeletal and Rheumatological Disorders at the University of Genoa.

Methods

This cross-sectional study, approved by the University of Genoa's Ethical Committee, utilized an anonymous web survey to gather data from physiotherapy students in the master's program. The survey, developed based on the International Handbook of Survey Methodology, consisted of 12 questions covering demographics, previous sEMG experience, the importance of sEMG in practice and research, and educational satisfaction. Data collection spanned from January to May 2024, with a response rate of 72.7% (93 participants). Descriptive analysis was used to summarize the data.

Results

The average age of respondents was 26.5 years, with 55.9% being male. Only 3.2% reported using sEMG in their practice. While 46.2% considered sEMG moderately important for practice, 40.9% deemed it extremely important for research. Most participants felt their undergraduate education inadequately prepared them for using sEMG, with 81.7% rating their preparation as insufficient. Although the master's program improved sEMG knowledge, 66.7% indicated no significant proficiency gain.

Conclusion

Italian physiotherapists view sEMG mainly as a research tool rather than a clinical one. The findings highlight the need for curriculum reforms to enhance both theoretical and practical sEMG education. Simplifying and standardizing sEMG protocols and integrating sEMG training into physiotherapy curricula are essential steps to better prepare clinicians for its clinical application.
The potential footprint of alternative meat adoption on corn and soybean producers
Authors: Blaustein-Rejto Dan , Merener Nicolas , Smith Alex
Published: October 2024

Alternative meat (AltMeat), which includes plant-based and cultured meats, has the potential to improve the global food supply chain on numerous environmental and societal dimensions. Some of these gains, derived from lower animal meat consumption, could disrupt the supply chain of crops that are used as animal feed. This study evaluates the potential impact of animal meat displacement on major corn and soybean-producing regions, globally or in developed regions. We combine trading patterns and regional cost structures with potential crop demand reductions caused by animal meat displacement, to occur by 2032 on top of an Organisation for Economic Co-operation and Development and the Food and Agriculture Organization of the United Nations (OECD-FAO) baseline projection without AltMeat. We find that potential animal meat displacement in the US and the European Union (EU) would have a minor effect on crop markets. Worldwide displacement, however, would lead to significantly lower corn and soybean prices relative to the baseline OECD-FAO projection without AltMeat. We explore quantitatively the heterogeneous impact of such developments in crop-producing regions. Our findings shed light on a trade-off associated with the significant benefits of more sustainable meat production.
Integrating 3D technology with the Sampaio classification for enhanced percutaneous nephrolithotomy in complex renal calculi treatment
Authors: Zhang Jiamo , Qing Jing , Hu Ke , Cheng Honglin
Published: October 2024

Background

To investigate the safety and efficacy of percutaneous nephrolithotomy (PCNL) in the treatment of complicated renal calculi by integrating three-dimensional (3D) computed tomography (CT) reconstruction with the Sampaio classification of the renal collecting system.

Methods

Sixty-four consecutive patients with complex kidney calculi who underwent PCNL between January 2019 and October 2023 were retrospectively analyzed and divided into experimental group (3D printing) and control group (CT imaging) according to their willingness to pay for 3D imaging. Both groups underwent preoperative CT urography. The Digital Imaging and Communications (DICOM) in Medicine data of the experimental group from CT imaging were used for 3D reconstruction and model printing. Then, the Sampaio classification system was used to design the puncture channel and develop a surgical strategy.

Results

The 3D-printed models of the experimental group successfully displayed the Sampaio classification system. There was no significant difference in the baseline parameters between the groups. Compared with the control group, the experimental group exhibited significant improvements in the puncture time, number of puncture needles, number of puncture channels, target calyx consistency, number of first puncture channels, and stone clearance. There were no significant differences in the total operative time, decrease in the hemoglobin level, length of hospital stay, and postoperative complications between the groups.

Conclusions

Integration of 3D technology with the Sampaio classification of the renal collecting system can enhance the preoperative evaluation and planning of percutaneous renal access. This approach allows a more precise method of PCNL for treating complex renal calculi.
Assessment of equine intestinal epithelial junctional complexes and barrier permeability using a monolayer culture system
Authors: Stewart Amy Stieler , Kopper Jamie J. , McKinney-Aguirre Caroline , Veerasamy Brittany , Sahoo Dipak Kumar , Freund John M. , Gonzalez Liara M.
Published: October 2024

Gastrointestinal disease is a leading cause of death in mature horses. A lack of in vitro modeling has impeded the development of novel therapeutics. The objectives of this study were to develop and further characterize a small intestinal monolayer cell culture derived from equine jejunum including establishing normal measurements of intestinal permeability and restitution. Three-dimensional enteroids, derived from postmortem sampling of equine jejunum, were utilized to develop confluent epithelial monolayers. The presence of differentiated intestinal epithelial cell types and tight junctions were confirmed using histology, reverse transcription PCR (RT-PCR), RNAscope, protein immunofluorescence and transmission electron microscopy. Transepithelial resistance (TER) and macromolecule flux were assessed as measurements of paracellular and transcellular permeability. Scratch assays were utilized to model and assess intestinal restitution. Monolayer cell cultures reached 100% confluency by ~5–7 days. Equine jejunum monolayers were confirmed as epithelial in origin, with identification of differentiated intestinal epithelial cell types and evidence of tight junction proteins. Function of the intestinal barrier was supported by acquisition of physiologically normal TER values (179.9 ± 33.7 ohms*cm2) and limited macromolecule flux (22 ± 8.8% at 60 min). Additionally, following a scratch wound, epithelial cell monolayers migrated to close gap defects within 24 h. In conclusion, this study describes the development of a novel intestinal epithelial monolayer cell culture for equine jejunum, and provides evidence of intestinal epithelial cell differentiation, formation of physiologically relevant barrier function and use as a model of intestinal restitution to test potential therapeutics for equine colic.
Autonomic regulation in athletic horses repetitively participating in two novice jumping classes on consecutive days
Authors: Wonghanchao Thita , Huangsaksri Onjira , Sanigavatee Kanokpan , Poochipakorn Chanoknun , Chanprame Sarisa , Wongkosoljit Sirapatch , Chotiyothin Wanlapa , Rattanayanon Nontaruj , Kiawwan Ratsamin , Chanda Metha
Published: October 2024

Introduction

Animal welfare is of great concern in equestrian sports and has been evaluated in athletic horses competing at different levels. However, the impact of consecutive days of jumping competition and the extent of resultant stress responses remains unclear. To address this point, the present study compared the changes in stress response via heart rate variability (HRV) in horses participating in two national jumping events on consecutive days.

Methods

The study involved six experienced horses equipped with heart rate monitoring devices. HRV variables were measured before, during, and after jumping at 10-min intervals for 60 min on each competition day.

Results

Multiple HRV variables decreased to varying degrees on both days from warm-up until 30 min post-jumping. Meanwhile, the mean heart rate increased during jumping and returned to normal levels at 50 min post-jumping on the first day (for all intervals, p < 0.05–0.001), while it remained elevated beyond 60 min post-jumping on the second day (for all intervals, p < 0.01–0.001). Additionally, maximum heart rate and respiratory rate were higher on the second day than in the first round during the warm-up phase (p < 0.05 for both variables). The proportion of the HRV low-frequency band was higher during riding on the second day (p < 0.05), while the proportion of the high-frequency band was reduced during warm-up on the first day (p < 0.05) and during course riding on the second (p < 0.01). Meanwhile, the sympathetic nervous system index took longer to return to baseline on the second day than on the first.

Discussion

These results suggest that autonomic regulation differed in horses between jumping rounds on two consecutive days, with horses experiencing higher sympathetic activity and potentially increased stress in the second round. This information is important for riders, highlighting the need to be mindful of potential stress that could, at least in part, impact the welfare of horses participating in the same jumping competition on consecutive days.
Deep Learning Approach for Arm Fracture Detection Based on an Improved YOLOv8 Algorithm
Authors: Meza Gerardo , Ganta Deepak , Gonzalez Torres Sergio
Published: October 2024

Artificial intelligence (AI)-assisted computer vision is an evolving field in medical imaging. However, accuracy and precision suffer when using the existing AI models for small, easy-to-miss objects such as bone fractures, which affects the models’ applicability and effectiveness in a clinical setting. The proposed integration of the Hybrid-Attention (HA) mechanism into the YOLOv8 architecture offers a robust solution to improve accuracy, reliability, and speed in medical imaging applications. Experimental results demonstrate that our HA-modified YOLOv8 models achieve a 20% higher Mean Average Precision (mAP 50) and improved processing speed in arm fracture detection.
Shifting Mindsets: Changes in Entrepreneurial Intention Among University Students
Authors: Xanthopoulou Panagiota , Sahinidis Alexandros , Kavoura Androniki , Antoniadis Ioannis
Published: October 2024

Despite the growing interest in entrepreneurship education and its impact on entrepreneurial intention, there is limited research on why students’ entrepreneurial intentions change over time. It has been observed that university students’ intentions to pursue entrepreneurship are higher when they enroll in entrepreneurship courses; however, these intentions usually shift both before and after the completion of the entrepreneurship course. This study investigates the shift in entrepreneurial intention among university students. Utilizing a mixed-method approach, this study combines the qualitative research tools of interviews and diaries to explore the evolution of students’ entrepreneurial intentions. The research sample consisted of undergraduate university students of a Greek university who had attended an entrepreneurship and innovation course as part of their first-year curriculum in the academic year 2020–2021. The study utilized 163 structured interviews and complementary diary research on 96 students from the same cohort in the academic year 2023–2024. NVivo12 software (32 bits version) was used to analyze the primary data. The findings highlight notable changes in students’ entrepreneurial intentions as a result of a variety of factors, including the allure of the entrepreneurial idea, cohesiveness of the teams, teaching strategies employed, support provided by the university and their families, and certain personality traits. These findings provide valuable insights for educators and policymakers to better support and sustain entrepreneurial intentions among students, while also further expanding the understanding of the strategy development needs of entrepreneurship counseling and education, as very limited research has been conducted on this issue.
Assessing the Effectiveness of Digital Marketing in Enhancing Tourist Experiences and Satisfaction: A Study of Thailand’s Tourism Services
Authors: Sharafuddin Mohammed Ali , Madhavan Meena , Wangtueai Sutee
Published: October 2024

Inbound digital marketing strategies play a vital role in the tourism industry, significantly influencing visitors’ decision-making processes, enhancing tourist experiences, and ensuring post-consumption satisfaction. This study explores the effectiveness of digital marketing strategies in shaping tourists’ perceptions of service quality and sustainability and their subsequent impact on overall satisfaction and behavioural intentions. This paper developed a conceptual framework and hypotheses through a comprehensive literature review to assess the causal relationships between digital marketing effectiveness, perceived destination service quality, perceived destination sustainability quality, tourist satisfaction, and behavioural intention. The empirical analysis was conducted using Partial Least Squares Structural Equation Modelling (PLS-SEM) on data collected from 195 international tourists in Thailand’s coastal regions. The results reveal that while digital marketing effectiveness directly influences tourists’ perceptions of service and sustainability quality, it indirectly influences tourist satisfaction and behavioural intentions through these perceptions. The findings highlight the critical role of aligning digital marketing strategies with service quality and sustainability initiatives. Moreover, the developed construct serves as a valuable tool for assessing and maintaining the destination image, helping tourism providers to improve their offerings and continuously foster long-term customer relationships.
Technology for Distribution and Control of Agrobacterium tumefaciens in Cherry Tree Soil
Authors: Liu Shenyan , Zheng Yiwen , Yu Kunpeng , Gao Shimeng , Zhao Xiaojuan , Cao Aocheng , Han Qingli
Published: October 2024

Sakura crown gall, caused by the invasion of Agrobacterium tumefaciens through plant wounds, poses a significant threat to cherry trees. In this study, the distribution of A. tumefaciens was preliminarily determined by stratified sampling and qPCR detection. Vertically, the pathogen is mainly distributed in the soil layer below 20 cm, and the amount of bacteria increases at greater depths. Horizontally, they are found within a 150 cm radius from the trunk. Zhongshengmycin and Oligosaccharide–Ethylicin were applied 100 cm from the trunk at a depth of below 20 cm. In the 20~40 cm soil layer, a 600-fold diluted solution of 3% Zhongshengmycin had a relative control efficacy of 94% to 100% against Agrobacterium tumefaciens, while a 1000-fold diluted solution of 25% Oligosaccharide–Ethylicin showed a control effect ranging from 54% to 100%. Before transplantation, the soil was disinfected with dazomet and abamectin. Application rates were 35 g/m2 for dazomet (98% granules) and 1 mL/m2 for abamectin (1.8% emulsifiable concentrate). The disinfection effectiveness was 77~100% in the 0~60 cm soil layer.
Wood-Based Biochar Ratio Used for Partial Peat Replacement in Growing Media for Antirrhinum majus Pot Production
Authors: Chrysargyris Antonios , Prasad Munoo , Tzortzakis Nikolaos
Published: October 2024

Biochar has been promoted mostly as a soil supplement that improves plant growth/yield and to a lesser extent as a growing medium component. The alarmed situation for peat substitution in growing medium renders biochar as a promising substitute for current research. In this study, biochar derived by wood-based materials was evaluated at different ratios (0, 5, 10, 15, and 20% v/v) for peat partial substitution for Antirrhinum majus pot production. Biochar had increased potassium content and pH, which affected the growing media properties (total pores space and water filled capacity) and decreased nitrogen and phosphorus content in the media. Adding ≥15% biochar increased plant height and decreased flowering, but no effect was observed on plant biomass produced. The presence of biochar increased the total phenols and flavanols content and antioxidant capacity, with greater effects at the higher biochar rates used. This resulted in lipid peroxidation and an increase in hydrogen peroxide content, causing oxidative stress. Potassium and magnesium accumulated more but nitrogen and phosphorus were accumulated less in snapdragon leaves. Biochar at 10% can be considered as a successful candidate to partially substitute peat, and efforts to improve growing media characteristics are required for A. majus pot production.
Effects of Different Fertilization Measures on Bacterial Community Structure in Seed Production Corn Fields
Authors: Yang Yirong , Zhao Zhenhua , Dong Bo , Zhang Rui , Jiang Jing , Ma Fengjie , Zhang Yingying , Zhao Jianhua , Du Dandan , Qiu Jindong , Li Chong
Published: October 2024

Different fertilization measures affect the soil’s physical and chemical properties and bacterial community structure, which in turn affects the growth environment and yield of maize seed production. Therefore, rational fertilization measures are important in maintaining and improving soil fertility and promoting maize crop growth. Research on fertilization practices in maize fields for seed production can help to increase agricultural productivity while protecting and enhancing soil health and achieving sustainable agricultural development. To clarify the effects of different fertilization measures on soil bacterial communities in seed corn fields, 16S rRNA high-throughput sequencing technology and PICRUSt method were used to explore the soil under different fertilization measures (CK as control, effects of single application of liquid organic fertilizer (M), single application of bacterial agents (BF), and combined application of liquid organic fertilizer and bacterial agents (M + BF)) on soil bacterial community structure characteristics and ecological functions. Proteobacteria (20.14–25.30%), Actinobacteriota (18.21–20.47%), Actinobacteriota (13.55–22.00%), and Chloroflexi (14.24–17.59%) were the dominant phyla. Bacillus, RB41, Arthrobacter, and Sphingomonas were the dominant genera. M + BF treatment significantly increased the relative abundance of Planctomycetota. The relative abundance of Bacillus and PaeniBacillus in M treatment decreased considerably, while the relative abundance of Turicibacter increased significantly. The relative abundance of Sphingomonas was reduced considerably in M and M + BF treatments. The relative abundance of Subgroup 10 decreased significantly after BF and M + BF treatments. BF treatment significantly increased the relative abundance of Skermanella. Redundancy analysis showed that alkali-hydrolyzed nitrogen (p = 0.044) was the main environmental factor affecting soil bacterial communities under different fertilizer treatments. PICRUSt function prediction results showed that metabolism was the main functional component of bacteria, accounting for 78.45–78.94%. The abundance of functional genes for terpenoid and polyketone metabolism, the endocrine system, the excretory system, and the immune system of the soil bacterial community was significantly increased under M treatment, while the abundance of functional genes for the digestive system was decreased considerably. Different fertilizer cultivation measures changed soil bacterial community composition and ecological function in maize fields. These results can provide a theoretical reference for the study of bacterial community succession characteristics in maize fields and the determination of appropriate fertilizer cultivation measures.
Enhanced Plant Growth Through Composite Inoculation of Phosphate-Solubilizing Bacteria: Insights from Plate and Soil Experiments
Authors: Li Mengsha , Li Xinjing , Xue Daosheng , Bao Chengjiang , Zhang Keying , Chen Lili , Li Qiuping , Guo Rui
Published: October 2024

Excessive application of phosphorus (P) fertilizers does not alleviate P deficiency in soils and may cause water eutrophication. The available P in acidic soils is bound to minerals, such as iron and aluminum, in forms that are difficult to utilize by plants. The low availability of P is detrimental to soil health and crop growth. To address the P imbalance in the soil, different bioremediation techniques, such as phosphate-solubilizing bacteria (PSB) application, have been employed. However, the systematic analysis of the effects of composite inoculation of PSB on crops remains elusive. In this study, the effects of composite-inoculated PSB on plant growth were systematically evaluated by two scales: plate experiment and soil test. This study employed six different strains of PSB including Lelliottia amnigena 1-1 (A), Kluyvera intermedia 1-2 (B), Pseudomonas tolaasii 1-6 (C), Burkholderia cepacia 2-5 (D), Pseudomonas frederiksbergensis 2-11 (E), and Pseudomonas rhodesiae 2-47 (F). Among the 57 different combinations of these strains, four combinations (AE, AF, ADF, and AEF) indicated higher phosphate-solubilizing abilities than the single strains. These combinations were used for subsequent experiments. The plate experiment revealed that composite strains were more effective than single strains in promoting the growth and development of seedlings and roots of oilseed rape. Furthermore, AE, AF, and AEF combinations indicated excellent growth-promoting effects. Moreover, the soil test revealed that the composite inoculation of AE and AEF significantly enhanced biomass accumulation and root development in oilseed rape. The increased growth-promoting effects of the composite strains were observed to be associated with to their phosphate-solubilizing capacities. Both scales confirmed that compared to single inoculation, composite inoculation of PSB is more beneficial for plant growth. This study provides composite inoculation materials and foundational data to support the bioremediation of P imbalance in soil.