bluuebunny/crossref_metadata_2025 huggingface dataset:
Situational Analysis and Tactical Decision-Making in Elite Handball Players
Authors: Nicolosi Simona , Quinto Antonella , Lipoma Mario , Sgrò Francesco
Published: August 2023

Situational analysis and decision-making represent key elements of elite sports performances, but few studies have investigated which player’s skills related to these aspects are relevant in elite handballers. The aim of this study was to address differences among handballers belonging to two tiers in processing situational probabilities information related to offensive and defensive situations. A total of 38 handballers (male = 22, female = 16, age: 25.6 ± 6.5 years, first-tier = 11, second-tier = 27) saw videos about different offensive and defensive actions. According to the temporal occlusion paradigm, each handballer provided a response about the best action a selected player had to perform according to the game’s context. The time, accuracy, and technical correctness of each player’s response were assessed. MANOVA revealed moderate-to-high skills differences between first- and second-tier players. First-tier players provided higher scores in response time and accuracy; they also obtained higher technical correctness scores in the most complex situation. The members of the first tier seemed to mainly depend on the accuracy of responses, even if the technical correctness also resulted in a predictor in the most complex situation. Playing in the best tier seems to require the development of very good skills related to processing situational probability information; therefore, training these elements seems to be necessary for determining the differences among elite handballers.
Five Decades of HBV Infection in Italy: A Continuous Challenge
Authors: Stroffolini Tommaso , Stroffolini Giacomo
Published: August 2023

In Italy, Hepatitis B virus (HBV) infection has been characterized by several changes over the last five decades. In 2019, the incidence of acute HBV among subjects targeted by the vaccination campaign was 0 cases in the age group 0–14 years and 0.1/100,000 in the age group 15–24. Nowadays, the burden of different stages of HBV-related chronic liver diseases is minimal. Intravenous drug use is no longer a risk factor (O.R. 0.7; 95% C.I. 0.5–1.02) for acquiring acute HBV; the proportion of cases reporting this exposure fell from 29.8% to 3.3% over the last two decades. The key public health intervention has been the compulsory vaccination campaign started in 1991 for infants 3 months old and 1–2 years old (the latter group for the first 12 years of the campaign). Moreover, non-immunogenic factors and the availability of effective oral antiviral drugs have played and continue to play a prominent role. The potential availability of new oral antiviral drugs with the inherent ability to eliminate the genomic HBV reservoirs may represent a further crucial step in the elimination of the virus in people that are already infected.
A Comparison of Cell-Cell Interaction Prediction Tools Based on scRNA-seq Data
Authors: Xie Zihong , Li Xuri , Mora Antonio
Published: August 2023

Computational prediction of cell-cell interactions (CCIs) is becoming increasingly important for understanding disease development and progression. We present a benchmark study of available CCI prediction tools based on single-cell RNA sequencing (scRNA-seq) data. By comparing prediction outputs with a manually curated gold standard for idiopathic pulmonary fibrosis (IPF), we evaluated prediction performance and processing time of several CCI prediction tools, including CCInx, CellChat, CellPhoneDB, iTALK, NATMI, scMLnet, SingleCellSignalR, and an ensemble of tools. According to our results, CellPhoneDB and NATMI are the best performer CCI prediction tools, among the ones analyzed, when we define a CCI as a source-target-ligand-receptor tetrad. In addition, we recommend specific tools according to different types of research projects and discuss the possible future paths in the field.
The Impact of Renal Function on Hepatic Encephalopathy Following TIPS Placement for Refractory Ascites
Authors: Zhao Matthew , Saab Sammy , Craw Chloe , Lee Edward Wolfgang
Published: August 2023

Background: The impact of renal function on hepatic encephalopathy (HE) following transjugular intrahepatic portosystemic shunt (TIPS) placement for refractory ascites is poorly understood. We investigated the role of renal function on HE following TIPS placement. Methods: A retrospective study was performed for patients undergoing TIPS for refractory ascites from 2007–2019. Patients were stratified by GFR at time of TIPS placement and by whether they were on hemodialysis (HD). Chronic kidney disease (CKD) stage 3 or higher was defined as pre-TIPS GFR < 60 for at least 3 months. Logistic regression analyses were used to identify the role of GFR and CKD at time of TIPS placement on HE within 60 days post TIPS placement. Results: Among 201 TIPS patients for refractory ascites (61% male; mean age 59.1), 78 (39%) patients were in CKD, and 16 (21%) were on HD. Mean GFR at time of TIPS placement was 62.7 ± 28.2 for all non-HD patients (n = 185). Compared with the GFR ≥ 90 group, GFR < 30 or HD (OR, 3.56; 95%CI, 1.19–10.7; p = 0.023) and CKD (OR, 2.52; 95%CI, 1.40–4.53; p = 0.002) at time of TIPS placement were significant predictors of post-TIPS placement HE within 60 days. GFRs between 30–60 and 60–90 were not significant predictors. Conclusions: In TIPS patients for recurrent ascites, patients with acutely impaired renal function or chronic renal dysfunction were at an increased risk for HE after TIPS.
Bioactive Compounds and In Vitro Antioxidant and Anticoccidial Activities of Opuntia ficus-indica Flower Extracts
Authors: Amrane-Abider Meriem , Imre Mirela , Herman Viorel , Debbou-Iouknane Nedjima , Zemouri-Alioui Salima , Khaled Souad , Bouiche Cilia , Nerín Cristina , Acaroz Ulaș , Ayad Abdelhanine
Published: August 2023

The objective of the present study is to identify the biochemical compounds extracted from OFI flowers using ultra-high-performance liquid chromatography–electrospray ionization quadrupole time-of-flight mass spectrometry and to evaluate their in vitro antioxidant activities and anticoccidial effects on the destruction of Eimeria oocysts isolated from naturally infected chickens. A domestic microwave was used with a refrigerant to condense the vapors generated during the extraction. The flavonoid and phenolic compound contents of the OFI flowers were determined according to standard methods. DPPH radical and H2O2 scavenging capacities were used to assess the antioxidant activity. Regarding the anticoccidial activity, the Eimeria spp. oocysts used were isolated from the fresh feces of infected broilers and were determined in triplicate by incubation at an ambient temperature for 24 h. The results highlighted the considerable influence of the optimized acetone concentration, ratio, irradiation time, and microwave power parameters on the phenolic content and antioxidant activities. Our results revealed significant matches between the predicted and experimental values of the models. Molecular analysis revealed the presence of several biophenol classes such as quercetin, isorhamnetin 3-O-rutinoside, and quercetin-3-O-rutinoside. OFI flower extracts inhibited sporulation and damaged the morphology of Eimeria oocysts compared with normal sporulated Eimeria oocysts containing sporocysts. In conclusion, the optimized conditions were validated and found to fit very well with the experimental values. These findings suggest that the flowers of OFI should be considered sources of antioxidants. The results of the present study revealed that OFI flower extracts have anticoccidial activities against Eimeria-spp.-induced infection in broiler chickens.
The Association between Serum Adiponectin Levels and Endothelial Function in Non-Dialysis-Dependent Chronic Kidney Disease Patients
Authors: Chen Ming-Chun , Lee Chung-Jen , Lin Yu-Li , Wang Chih-Hsien , Hsu Bang-Gee
Published: August 2023

Adiponectin is the richest human circulating adipokine with anti-inflammatory, antioxidant, and insulin-sensitizing effects. We evaluated the association between serum adiponectin levels and endothelial function in chronic kidney disease (CKD) patients, obtaining fasting blood samples from 130 non-dialysis CKD subjects. We measured the endothelial function—represented by the vascular reactivity index (VRI)—via non-invasive digital thermal monitoring, and serum adiponectin concentrations by enzyme immunoassay kits. A total of 22 (16.9%), 39 (30.0%), and 69 (53.1%) patients had poor (VRI < 1.0), intermediate (1.0 ≤ VRI < 2.0), and good (VRI ≥ 2.0) vascular reactivity. Elevated serum blood urea nitrogen (BUN) level was negatively correlated with VRI values, but serum adiponectin and estimated glomerular filtration rate were positively associated with VRI values by univariate linear regression analysis. After applying multivariate stepwise linear regression analysis adjustment, the significantly positive association of adiponectin (p < 0.001), and the significantly negative association of log-BUN (p = 0.021) with VRI values in CKD subjects remained. In an animal study using in vitro blood-vessel myography, treatment with adiponectin enhancing acetylcholine-mediated vasorelaxation in 5/6 nephrectomy CKD mice. Our study results indicated that adiponectin concentration was positively associated with VRI values and modulated endothelial function in non-dialysis CKD patients.
Body Mass Index and Its Influence on Chronic Low Back Pain in the Spanish Population: A Secondary Analysis from the European Health Survey (2020)
Authors: Lucha-López María Orosia , Hidalgo-García César , Monti-Ballano Sofía , Márquez-Gonzalvo Sergio , Ferrández-Laliena Loreto , Müller-Thyssen-Uriarte Julián , Lucha-López Ana Carmen
Published: August 2023

Risk factors such as obesity and a sedentary lifestyle contribute to the development of chronic low back pain. Purpose: To describe how body mass index (BMI) influences the prevalence of chronic low back pain in the general Spanish population and assess this influence given other factors as sex, age, physical occupational demands, and recreational physical activity. Methods: An analytical cross-sectional design was performed based on secondary data from the European Health Survey in Spain (2020). Data on age, gender, physical occupational demands, physical activity, BMI, and presence of chronic low back pain were extracted. Results: A total of 19,716 (52.2% women) subjects with a median age of 53 years old were analyzed. Of these, 18.3% had chronic low back pain, 39% were overweight, and 16.1% were obese. The adjusted generalized linear model showed that being obese increased the odds of chronic low back pain by 1.719 times (p < 0.001). Being a woman increased the odds by 1.683 times (p < 0.001). Performing occupational tasks requiring high physical demands increased the odds by 1.403 times (p < 0.001). Performing physical activity in leisure time several times a week decreased the odds by 0.598 times (p < 0.001). For every additional year of age, the odds of chronic low back pain increased by 3.3% (p < 0.001). Conclusions: Obesity is related with the presence of chronic low back pain in the general Spanish population. This association persists, being the more relevant factor, after adjusting the association of BMI and chronic low back pain with other factors such as sex, age, physical occupational demands, and recreational physical activity.
Immunoinformatics for Novel Multi-Epitope Vaccine Development in Canine Parvovirus Infections
Authors: Paul Bashudeb , Alam Jahangir , Hossain Mridha Md. Kamal , Hoque Syeda Farjana , Bappy Md. Nazmul Islam , Akter Hafsa , Ahmed Nadim , Akter Margia , Ali Zinnah Mohammad , Das Shobhan , Mia Md. Mukthar , Parvej Md. Shafiullah , Sarkar Sonjoy , Ghosh Hiren , Hasan Mahmudul , Ashour Hossam M. , Rahman Md. Masudur
Published: August 2023

Canine parvovirus (CPV-2) is one of the most important pathogens of dogs of all ages, causing pandemic infections that are characterized by fatal hemorrhagic enteritis. The CPV-2 vaccine is recommended as a core vaccine for pet animals. Despite the intensive practice of active immunization, CPV-2 remains a global threat. In this study, a multi-epitope vaccine against CPV-2 was designed, targeting the highly conserved capsid protein (VP2) via in silico approaches. Several immunoinformatics methods, such as epitope screening, molecular docking, and simulation were used to design a potential vaccine construct. The partial protein sequences of the VP2 gene of CPV-2 and protein sequences retrieved from the NCBI were screened to predict highly antigenic proteins through antigenicity, trans-membrane-topology screening, an allergenicity assessment, and a toxicity analysis. Homologous VP2 protein sequences typically linked to the disease were identified using NCBI BLAST, in which four conserved regions were preferred. Overall, 10 epitopes, DPIGGKTGI, KEFDTDLKP, GTDPDDVQ, GGTNFGYIG, GTFYFDCKP, NRALGLPP, SGTPTN, LGLPPFLNSL, IGGKTG, and VPPVYPN, were selected from the conserved regions to design the vaccine construct. The molecular docking demonstrated the higher binding affinity of these epitopes with dog leukocyte antigen (DLA) molecules. The selected epitopes were linked with Salmonella enterica flagellin FliC adjuvants, along with the PADRE sequence, by GGS linkers to construct a vaccine candidate with 272 nucleotides. The codon adaptation and in silico cloning showed that the generated vaccine can be expressed by the E. coli strain, K12, and the sequence of the vaccine construct showed no similarities with dog protein. Our results suggest that the vaccine construct might be useful in preventing canine parvoviral enteritis (CPE) in dogs. Further in vitro and in vivo experiments are needed for the validation of the vaccine candidate.
Effective Feature Engineering and Classification of Breast Cancer Diagnosis: A Comparative Study
Authors: Strelcenia Emilija , Prakoonwit Simant
Published: August 2023

Breast cancer is among the most common cancers found in women, causing cancer-related deaths and making it a severe public health issue. Early prediction of breast cancer can increase the chances of survival and promote early medical treatment. Moreover, the accurate classification of benign cases can prevent cancer patients from undergoing unnecessary treatments. Therefore, the accurate and early diagnosis of breast cancer and the classification into benign or malignant classes are much-needed research topics. This paper presents an effective feature engineering method to extract and modify features from data and the effects on different classifiers using the Wisconsin Breast Cancer Diagnosis Dataset. We then use the feature to compare six popular machine-learning models for classification. The models compared were Logistic Regression, Random Forest, Decision Tree, K-Neighbors, Multi-Layer Perception (MLP), and XGBoost. The results showed that the Decision Tree model, when applied to the proposed feature engineering, was the best performing, achieving an average accuracy of 98.64%.
Methylene-Blue-Encapsulated Metal-Organic-Framework-Based Electrochemical POCT Platform for Multiple Detection of Heavy Metal Ions in Milk
Authors: Xiong Huiwen , Li Pintao , Cun Fei , Chen Hui , Kong Jilie
Published: August 2023

Considering the high risk of heavy metal ions (HMIs) transferring through the food chain and accumulating in milk, a flexible and facile point-of-care testing (POCT) platform is urgently needed for the accurate, sensitive, and highly selective on-site quantification of multiple HMIs in milk. In this work, a cost-effective disk with six screen-printed electrodes (SPEs) was designed for hand-held electrochemical detection. Metal organic frameworks (MOFs) were adopted to amplify and enhance the electrochemical signals of methylene blue (MB). Using differential pulse voltammetry (DPV) methods, low limits of detection for four HMIs (Cd2+, 0.039 ppb; Hg2+, 0.039 ppb; Pb2+, 0.073 ppb; and As3+, 0.022 ppb) were achieved within four minutes. Moreover, the quantitative POCT system was applied to milk samples. The advantages of low cost, ease of on-site implementation, fast response, and accuracy allow for the POCT platform to be used in practical monitoring applications for the quantitation of multiple HMIs in milk samples.
From Building Information Model to Digital Twin: A Framework for Building Thermal Comfort Monitoring, Visualizing, and Assessment
Authors: Desogus Giuseppe , Frau Caterina , Quaquero Emanuela , Rubiu Giulia
Published: August 2023

The existing building stock is globally responsible for 17.5% of greenhouse gas emissions due to their operation to achieve occupant satisfaction, thus requiring a vast intervention. However, reducing building stock emissions and optimizing building energy performance cannot be considered independently by the users’ well-being. The thermal comfort conditions and their monitoring represent a central issue that could optimize building energy usage while achieving good indoor environmental conditions. This document describes the first findings of ongoing research focused on the development of a building monitoring system, based on the integration of Building Information Modeling tools and sensor technology through Dynamo Visual Programming. Starting from the development of an Asset Information Model, which represents the virtual replica of a building that currently hosts the administrative offices of the municipality of Cagliari, the first step presented in this contribution shows a thermal comfort monitoring system, scalable and modular, that allows effective gathering and elaboration of data about comfort levels in each of the building’s rooms. The system proves to be a helpful support for facility managers to control building thermal comfort conditions and HVAC systems to assure their best operative status or plan suitable interventions to achieve it.
A Review on Damage Monitoring and Identification Methods for Arch Bridges
Authors: Yang Jiafeng , Huang Lei , Tong Kai , Tang Qizhi , Li Houxuan , Cai Haonan , Xin Jingzhou
Published: August 2023

The damage monitoring and identification of arch bridges provide an important means to ensure the safe operation of arch bridges. At present, many methods have been developed, and the applicability and effectiveness of these methods depend on the damage type, structural configuration and available data. To guide the practical application of these methods, a systematic review is implemented in this paper. Specifically, the damage monitoring and identification methods of arch bridges are divided into the damage monitoring of local diseases and damage identification of overall performance. Firstly, the research on the damage monitoring of the local diseases of arch bridges is reviewed. According to the disease type, it is divided into four categories, including suspender inspection, void monitoring, stress detection and corrosion detection. For each disease, this paper analyzes the principles, advantages and shortcomings of various methods. Then, the damage identification methods of the overall performance of arch bridges are reviewed, including masonry arch bridges, steel arch bridges, reinforced concrete arch bridges and concrete-filled steel tubular arch bridges. And the commonly used damage indexes of damage identification methods are summarized. This review aims to help researchers and practitioners in implementing existing damage detection methods effectively and developing more reliable and practical methods for arch bridges in the future.
Human-Induced Vibration Serviceability: From Dynamic Load Measurement towards the Performance-Based Structural Design
Authors: Wang Haoqi , Ge Qian , Zeng Dongjun , Zhang Zhuoran , Chen Jun
Published: August 2023

Since the well-known Millennium bridge accident happened at the beginning of this century, both researchers and engineers realized that the human-induced vibration may lead to unaffordable consequences. Although such vibrations hardly threaten the safety of the structure, the large vibration may affect the functionalities of the structure, causing the serviceability problem. The first study on the human-induced vibration serviceability problem started from the measurement of human-induced load, with many mathematical models proposed. The strong randomness of the measured data led to the investigation on the randomness feature of the load. With the research going deeper, the phenomenon of human–structure interaction was found, which attracted the researchers to study the randomness of the human body dynamic properties that may affect the structural response. Once the interaction mechanism and the system parameters became available, random vibration analysis methods could be proposed to calculate human-induced random vibration, providing the foundation of the reliability analysis from the perspective of vibration serviceability. Such reliability is highly related to subjective feelings of the human body, which has also been deeply studied in the literature. Furthermore, the purpose of studying the dynamic reliability is to conduct the reliability-based structural design. This paper provides a review of the research on human-induced vibration serviceability following the above logic, from the first attempt on load measurement towards the modern techniques for performance-based vibration serviceability design.
Flexural Experiment and Design Method of Steel-Wire-Enhanced Insulation Panels
Authors: Jiang Jinliang , Xu Linyi , Zhang Enyuan , Hou Jian , Tong Jingzhong
Published: August 2023

A new type of non-dismantling composite insulation panel, namely a steel-wire-enhanced insulation panel, was proposed. Compared to traditional organic insulation panels, the construction procedure is reduced, and the fire resistance is improved. The flexural performance was explored experimentally and numerically to evaluate its ability to withstand lateral pressure when it was used as the formwork of a cast-in-place concrete wall. First, 6 groups of 12 specimens of steel-wire-enhanced insulation panels were conducted under 2 loading modes: 3-point bending loading and 4-point bending loading. The failure modes of these specimens included a straight crack at the bottom of the panel and the yielding of steel wire. The test results showed that the maximum bending moment of the specimens with an 80 mm thickness could reach 2.415 kN·m. Second, finite element (FE) models were developed for the steel-wire-enhanced insulation panels by ABAQUS, which were validated by the experimental results. Third, a parametric study with parameters, including the thermal insulation cover, the square gird spacing of the steel wire mesh, and the diameter of the steel wire, was performed. It was observed that the insulation cover had a significant effect on the flexural capacity in the simulated range. Finally, theoretical formulas for panel stiffness and flexural capacity were presented, which can predict the bending performance more conservatively compared to the experimental results. The research and analysis of this study could offer a valuable reference for designing this panel in practical applications.
Dedifferentiated Chondrosarcoma from Molecular Pathology to Current Treatment and Clinical Trials
Authors: Zając Weronika , Dróżdż Julia , Kisielewska Weronika , Karwowska Weronika , Dudzisz-Śledź Monika , Zając Agnieszka E. , Borkowska Aneta , Szumera-Ciećkiewicz Anna , Szostakowski Bartłomiej , Rutkowski Piotr , Czarnecka Anna M.
Published: August 2023

Dedifferentiated chondrosarcoma (DDCS) is a rare subtype of chondrosarcoma, a primary cartilaginous malignant neoplasm. It accounts for up to 1–2% of all chondrosarcomas and is generally associated with one of the poorest prognoses among all chondrosarcomas with the highest risk of metastasis. The 5-year survival rates range from 7% to 24%. DDCS may develop at any age, but the average presentation age is over 50. The most common locations are the femur, pelvis humerus, scapula, rib, and tibia. The standard treatment for localised disease is surgical resection. Most patients are diagnosed in unresectable and advanced stages, and chemotherapy for localised and metastatic dedifferentiated DDCS follows protocols used for osteosarcoma.
Skin Involvement by Hematological Neoplasms with Blastic Morphology: Lymphoblastic Lymphoma, Blastoid Variant of Mantle Cell Lymphoma and Differential Diagnoses
Authors: Zanelli Magda , Sanguedolce Francesca , Zizzo Maurizio , Fragliasso Valentina , Broggi Giuseppe , Palicelli Andrea , Loscocco Giuseppe Gaetano , Cresta Camilla , Caprera Cecilia , Corsi Matteo , Martino Giovanni , Bisagni Alessandra , Marchetti Marialisa , Koufopoulos Nektarios , Parente Paola , Caltabiano Rosario , Ascani Stefano
Published: August 2023

Hematological neoplasms sharing a blastic morphology may involve the skin. The skin may be either the primary site of occurrence of hematological malignancies with blastic features or cutaneous lesions are the first manifestation of an underlying systemic malignancy. The assessment of skin biopsies of hematological neoplasms with blastic features poses diagnostic problems and requires expert hematopathologists considering a wide range of differential diagnoses. The precise diagnosis of diseases sharing blastic features but with different outcomes and requiring distinct therapies is essential for patient management. The present paper mainly focuses on cutaneous involvement of the blastoid variant of mantle cell lymphoma and lymphoblastic lymphoma of B-cell or T-cell origin. The relevant literature has been reviewed and the clinical aspects, pathological features, prognosis, and therapy of both blastoid mantle cell lymphoma and lymphoblastic lymphoma involving the skin are discussed. A focus on other hematological entities with blastic features, which may involve the skin, to be taken into consideration in differential diagnosis is also given.
Pituitary Stalk Morphology as a Predictor of New-Onset Adrenocortical Insufficiency and Arginine Vasopressin Deficiency after Transsphenoidal Resections of Pituitary Macroadenomas: A Retrospective Single-Center Study with a Focus on iMRI
Authors: Becker Ralf , Hlavac Michal , Etzrodt-Walter Gwendolin , Sommer Fabian , Wirtz Christian Rainer , Schmitz Bernd , Pala Andrej
Published: August 2023

Background: A new-onset adrenocortical insufficiency (NAI) is the most critical postoperative endocrinological complication after transsphenoidal surgery for macroadenomas. Because of increased mortality risk, arginine vasopressin deficiency (AVP-D) is also a relevant postoperative complication. This study aimed to identify easy-to-acquire magnet resonance imaging (MRI) aspects of the pituitary stalk to predict these insufficiencies after transsphenoidal surgery. Methods: Pituitary stalk morphology was reviewed intraoperatively and three months postoperatively in the MRIs of 48 transsphenoidal surgeries for macroadenomas. NAI was validated in endocrinological follow-up controls 10–14 months post-surgery. Results: Intraoperative pituitary stalk diameters were 0.5 mm larger in patients who developed NAI and AVP-D. The odds ratio was 29 for NAI and 6 for AVP-D in binary regression analysis. A value of 2.9 mm was identified as the optimal cut-off for the minimal pituitary stalk diameter regarding NAI, with a high specificity of 89%. There was no difference in pituitary stalk diameter regarding these insufficiencies three months post-surgery. Conclusions: We identified an increased pituitary stalk diameter in intraoperative MRIs as a predictive factor of NAI and AVP-D after transsphenoidal surgery. These findings might improve the early detection of NAI and, thus, optimal management. However, validating these retrospective findings in prospective studies is obligatory.
Cordycepin (3′dA) Induces Cell Death of AC133+ Leukemia Cells via Re-Expression of WIF1 and Down-Modulation of MYC
Authors: Abazari Nazanin , Stefanucci Marta Rachele , Bossi Luca Emanuele , Trojani Alessandra , Cairoli Roberto , Beghini Alessandro
Published: August 2023

Wnt/β-catenin signaling is critically required for the development and maintenance of leukemia stem cells (LSCs) in acute myeloid leukemia (AML) by constitutive activation of myeloid regeneration-related pathways. Cell-intrinsic activation of canonical Wnt signaling propagates in the nucleus by β-catenin translocation, where it induces expression of target oncogenes such as JUN, MYC and CCND1. As the Wnt/β−catenin pathway is now well established to be a key oncogenic signaling pathway promoting leukemic myelopoiesis, targeting it would be an effective strategy to impair LSC functionality. Although the effects of the adenosine analogue cordycepin in repressing β-catenins and destabilizing the LSC niche have been highlighted, the cellular and molecular effects on AML–LSC have not been fully clarified. In the present study, we evaluated the potency and efficacy of cordycepin, a selective repressor of Wnt/β-catenin signaling with anti-leukemia properties, on the AC133+ LSC fraction. Cordycepin effectively reduces cell viability of the AC133+ LSCs in the MUTZ−2 cell model and patient-derived cells through the induction of apoptosis. By Wnt-targeted RNA sequencing panel, we highlighted the re-expression of WIF1 and DKK1 among others, and the consequent downregulation of MYC and PROM1 (CD133) following MUTZ−2 cell exposure to increasing doses of cordycepin. Our results provide new insights into the molecular circuits involved in pharmacological inhibition mediated by cordycepin reinforcing the potential of targeting the Wnt/β-catenin and co-regulatory complexes in AML.
Breast Sarcomas, Phyllodes Tumors, and Desmoid Tumors: Turning the Magnifying Glass on Rare and Aggressive Entities
Authors: Esperança-Martins Miguel , Melo-Alvim Cecília , Dâmaso Sara , Lopes-Brás Raquel , Peniche Tânia , Nogueira-Costa Gonçalo , Abreu Catarina , Luna Pais Helena , de Sousa Rita Teixeira , Torres Sofia , Gallego-Paez Lina Marcela , Martins Marta , Ribeiro Leonor , Costa Luís
Published: August 2023

Breast sarcomas (BSs), phyllodes tumors (PTs), and desmoid tumors (DTs) are rare entities that arise from connective tissue. BSs can be classified as either primary or secondary, whether they develop de novo or after radiation exposure or lymphedema. PIK3CA seems to play an important common role in different BS. Malignant PTs show similar behavior to BSs, while DTs are locally aggressive but rarely metastasize. BSs usually present as unilateral, painless, rapidly growing masses with rare nodal involvement. The diagnosis should be based on magnetic resonance imaging and a core needle biopsy. Staging should comprise a chest computed tomography (CT) scan (except for benign PT and DT), while abdominal and pelvic CT scans and bone scans should be added in certain subtypes. The mainstay of treatment for localized BS is surgery, with margin goals that vary according to subtype. Radiotherapy and chemotherapy can be used as neoadjuvant or adjuvant approaches, but their use in these settings is not standard. Advanced BS should be treated with systemic therapy, consistent with recommendations for advanced soft tissue sarcomas of other topographies. Given the rarity and heterogeneity of these entities, multidisciplinary and multi-institutional collaboration and treatment at reference centers are critical.
Incidence of ‘Low-Risk but Not No-Risk’ Features of Cancer Prior to High-Risk Feature Occurrence: An Observational Cohort Study in Primary Care
Authors: Moore Sarah F. , Price Sarah J. , Bostock Jennifer , Neal Richard D. , Hamilton Willie
Published: August 2023

Diagnosing cancer may be expedited by decreasing referral risk threshold. Clinical Practice Research Datalink participants (≥40 years) had a positive predictive value (PPV) ≥3% feature for breast, lung, colorectal, oesophagogastric, pancreatic, renal, bladder, prostatic, ovarian, endometrial or laryngeal cancer in 2016. The numbers of participants with features representing a 1–1.99% or 2–2.99% PPV for same cancer in the previous year were reported, alongside the time difference between meeting the ≥3% criteria and the lower threshold criteria. A total of 8616 participants had a PPV ≥3% feature, of whom 365 (4.2%) and 1147 (13.3%), respectively, met 2–2.99% and 1–1.99% criteria in the preceding year. The median time difference was 131 days (Interquartile Range (IQR) 27 to 256) for the 2–2.99% band and 179 days (IQR 58 to 289) for the 1–1.99% band. Results were heterogeneous across cancer sites. For some cancers, participants may progress from presenting lower- to higher-risk features before meeting urgent referral criteria; however, this was not usually the case. The details of specific features across multiple cancer sites will allow for a tailored approach to future reductions in referral thresholds, potentially improving the efficiency of urgent cancer referrals for the benefit both of individuals and the National Health Service (NHS).
Consolidative Radiotherapy after Complete Remission following R-CHOP Immunochemotherapy in Stage III–IV Diffuse Large B-Cell Lymphoma Patients: A Systematic Review and Meta-Analysis
Authors: Choi Kyu-Hye , Lee Seung-Jae , Mun So-Hwa , Song Jin-Ho , Choi Byung-Ock
Published: August 2023

Patients with diffuse large B-cell lymphoma (DLBCL) are treated with rituximab in combination with cyclophosphamide, doxorubicin, vincristine, and prednisone (R-CHOP). The role of consolidative radiation therapy (RT) remains unclear among patients with advanced DLBCL who achieved complete remission (CR) after R-CHOP immunochemotherapy. The current systematic review and meta-analysis aimed to clarify the role of consolidative RT among these patients. The MEDLINE, Embase, and Cochrane Library databases were searched for studies comparing RT to no RT following CR after R-CHOP immunochemotherapy in Ann Arbor stage III–IV DLBCL patients. Overall survival (OS) was the primary endpoint, and disease-free survival (DFS) was the secondary endpoint. Hazard ratios (HRs) and 95% confidence intervals (CIs) were calculated to assess the primary and secondary outcomes. Review Manager (version 5.4) was used to analyze the data. Six retrospective studies involving 813 patients who received R-CHOP ± consolidative RT were identified. OS was higher in the consolidative RT group, with an HR of 2.01 and a 95% CI of 1.30 to 3.12 (p = 0.002). DFS was also higher in the RT group, with an HR of 2.18 and a 95% CI of 1.47 to 3.24 (p < 0.0001). The results suggested that consolidative RT improved OS and DFS compared to no RT among advanced-stage DLBCL patients. Further research is needed to determine the optimal radiation fields and the appropriate indications for consolidative RT for advanced-stage DLBCL patients in the rituximab era.
Synthesis of Tungsten-Modified Sn3O4 through the Cetyltrimethylammonium Bromide-Assisted Solvothermal Method for Dye Decolorization under Visible Light Irradiation
Authors: Furukawa Mai , Iwamoto Daichi , Inamori Koki , Tateishi Ikki , Katsumata Hideyuki , Kaneco Satoshi
Published: August 2023

Novel tungsten-modified mixed-valence tin oxides (Sn3O4) with two oxidation numbers, such as Sn2+ and Sn4+, were successfully prepared by the cetyltrimethylammonium bromide (CTAB)-assisted solvothermal method in one-step using tin (II) chloride dihydrate and sodium tungstate (IV) dihydrate as the precursors for dye degradation of methyl orange (MO) under visible light irradiation. The synthesized materials were characterized by various techniques to investigate the surface/structural morphology and the optical property. The presence of tungsten and the optimized amount of CTAB in the preparation method were favorable for the photocatalytic dye degradation reaction. In particular, when 0.03 of CTAB was added to W-modified Sn3O4 (W-Sn3O4@CTAB) and its concentration was 0.6 mg/mL, 10 mg/L of MO could be decolorized almost completely in 40 min, with the apparent reaction rate constant of 0.0496 min−1. The improvement of photocatalytic activity for this proposed W-Sn3O4 results from increased reduction power, enhanced separation of electron–hole pairs, extended visible light absorption range, and optimized band structure by CTAB additive. The radical trapping experiments showed that the main reactive species during the photocatalytic reaction are superoxide ions. The developed photocatalysts may contribute to the development of environmental improvement technology.
Development of a New Hydrogen Sulfide Fluorescent Probe Based on Coumarin–Chalcone Fluorescence Platform and Its Imaging Application
Authors: Chi Hanwen , Gu Lei , Zhang Qian , Tang Yonghe , Guo Rui , Lin Weiying
Published: August 2023

Hydrogen sulfide (H2S), as one of the critical gaseous signaling molecules, has important physiological functions in the human body, and abnormal levels of hydrogen sulfide are closely related to tumors, Parkinson’s disease, Alzheimer’s disease, and other diseases. In order to enable the detection of H2S in the physiological environment, herein, a new H2S fluorescence probe, named C-HS, based on a coumarin–chalcone fluorescence platform was developed. The fluorescence probe provides specific recognition of H2S within a wide pH detection range (5.5–8.5), a rapid recognition response (within 10 min) for H2S molecules, and a high selectivity for competing species. The probe C-HS possesses low cytotoxicity and is used to achieve the detection of exogenous/ endogenous H2S in living cells, indicating that the constructed probe C-HS has the ability to track changes in intracellular H2S levels. Therefore, probe C-HS could be a potential tool for the early diagnosis of H2S-related diseases.
A Pilot Study of Family-Integrated Care (FICare) in Critically Ill Preterm and Term Infants in the NICU: FICare Plus
Authors: Ansari Najmus Sehr , Franck Linda S. , Tomlinson Christopher , Colucci Anna , O’Brien Karel
Published: August 2023

Family-integrated care (FICare) is associated with improved developmental outcomes and decreased parental mental health risks in stable preterm infants. However, less is known about its application in critically ill infants who are at greater risk for adverse outcomes. The objective of this study was to assess the safety and feasibility of implementation of an augmented FICare program, FICare Plus, in critically ill infants in the first few weeks of life. Resources were specifically developed for staff and parents to support earlier parental engagement in infant care. Infant health outcomes and standardized measures of parental stress, anxiety and parenting self-efficacy were also collected using standardized questionnaires: State -Trait Anxiety Inventory (STAI), Parental Stressor Scale: NICU (PSS: NICU), Perceived Parenting Self-Efficacy Tool and Family Centered Care Survey. The t-test or Wilcoxon rank-sum test were used to compare continuous variables, while the Chi-square or Fisher exact test were used for categorical variables, respectively. In this prospective cohort study, 41 critically ill infants were enrolled: 17 in standard care (SC) and 24 in the FICare Plus group. The tools and procedures developed for FICare Plus successfully supported greater engagement in the care of their infants with no increase in adverse events and no increase in parental stress. Parents in the FICare Plus cohort felt confident to participate in their infant’s care. The staff also found this model of care acceptable and well adopted. Preliminary measures of infant efficacy were similar in both groups. Total anxiety scores were high among all parents at enrollment (87 (67–94) vs. 70.5 (66–86); p-value 0.22). However, the scores prior to discharge were lower in FICare Plus group (78 (71–90) vs. 63 (52–74.5); p-value 0.02). This pilot study showed that it is feasible and safe to implement family-integrated care in critically ill infants.
Effects of Er,Cr: YSGG Laser Application in De-Bonding of Different Ceramic Veneer Materials (In Vitro Study)
Authors: Amin Bassam Karem
Published: August 2023

Background: Ceramic restorations are widely used nowadays as the esthetic demand has increased in the worldwide population, and lithium disilicate and feldspathic porcelain materials are the most widely used veneer materials. The traditional removal procedure for veneers was recently replaced with the use of laser technology to debond the veneers so that the de-bonded veneers can be preserved and re-used. Aim: Up to now, there have been few studies regarding using lasers to remove ceramic laminate; thus, it is clear why this research topic is important for examining the efficiency of lasers in the use of de-bonding for different ceramic laminates with varying compositions and materials. Materials and Methods: This study employed forty-five normal human maxillary first premolars with comparable proportions. The forty-five teeth were initially split into three groups of fifteen teeth, and the teeth were chosen at random. Each group fused pairs of various ceramic materials. A total of fifteen teeth in the first group underwent feldspathic porcelain restorations. The second group contained fifteen restorations made of lithium disilicate glass-ceramic CAD-CAM. In the third group, fifteen teeth were restored using glass-ceramic that had a lithium disilicate glass-ceramic ingot. The RelyX Veneer A1 shade, available from 3M EPSE in the United States, was used to bond all the samples. The specimens were then submerged for approximately 24 h in distilled water at 37 degrees Celsius in order to simulate the conditions in an oral cavity. An Er,Cr: YSGG laser (Waterlase, iPlus, Biolase, from USA) was used with a turbo headpiece and an MX7 sapphire tip for irradiation. Results: The time needed for the ceramic disc to debond was calculated using a digital stopwatch. The average removal times for the feldspathic porcelain, lithium disilicate glass-ceramic ingot, and lithium disilicate glass-ceramic CAD-CAM were 10.067 ± 1.668 s, 5.200 ± 1.146 s, and 5.133 ± 1.125 s, while the removal times ranged from 8–12 s, 4–7 s, and 4–7 s, respectively. Compared to the other ceramic materials, de-bonding the feldspathic porcelain took longer. Conclusions: According to this study, the Er,Cr: YSGG laser application using the same study parameters made it simpler to de-bond lithium disilicate and feldspathic porcelain. Lithium disilicate, as opposed to feldspathic porcelain, transmits laser light more effectively; hence, the results varied depending on the materials, and the debonding occurred primarily at the veneer–cement interface.
Transforming Chimney Soot via Stochastic Polymerization for Active Electrode Coating
Authors: Petrov Miroslav , Slavov Lyubomir , Stankulov Toma , Karamanova Boryana , Milenov Teodor , Dimov Dimitar , Avramova Ivalina
Published: August 2023

A polymerization procedure is presented to increase the molecular weight of hydrocarbons in household chimney soot without thermal treatment at high temperatures. Pristine soot was subject to chlorination, with half of it treated with magnesium (Mg-plates) to create random-type Grignard reagents (R-Mg-Cl) in diethyl ether media. Mixing the Grignard reagent and the rest of the halogenated soot material created new C-C bonds, thus increasing the molecular weight of the final product. The obtained stochastically polymerized soot (SPS) was investigated using Raman spectroscopy, FTIR spectroscopy and XPS and was subjected to electrochemical testing as an assembled supercapacitor with a KOH electrolyte. Results show significant carbon structure differences due to the chemical procedures and newly created functional groups in the soot. Such functional groups could increase the capacity of supercapacitors, creating pseudo-capacitance by participating in redox reactions. The results also unveiled removing any random contaminations in the pristine soot and obtaining a more uniform final product containing hydrocarbons with longer chains, thus increasing the molecular weight.
A Comprehensive Approach to Image Protection in Digital Environments
Authors: Villegas-Ch William , García-Ortiz Joselin , Govea Jaime
Published: August 2023

Protecting the integrity of images has become a growing concern due to the ease of manipulation and unauthorized dissemination of visual content. This article presents a comprehensive approach to safeguarding images’ authenticity and reliability through watermarking techniques. The main goal is to develop effective strategies that preserve the visual quality of images and are resistant to various attacks. The work focuses on developing a watermarking algorithm in Python, implemented with embedding in the spatial domain, transformation in the frequency domain, and pixel modification techniques. A thorough evaluation of efficiency, accuracy, and robustness is performed using numerical metrics and visual assessment to validate the embedded watermarks. The results demonstrate the algorithm’s effectiveness in protecting the integrity of the images, although some attacks may cause visible degradation. Likewise, a comparison with related works is made to highlight the relevance and effectiveness of the proposed techniques. It is concluded that watermarks are presented as an additional layer of protection in applications where the authenticity and integrity of the image are essential. In addition, the importance of future research that addresses perspectives for improvement and new applications to strengthen the protection of the goodness of pictures and other digital media is highlighted.
Is the Privacy Paradox a Domain-Specific Phenomenon
Authors: Hirschprung Ron S.
Published: August 2023

The digital era introduces significant challenges for privacy protection, which grow constantly as technology advances. Privacy is a personal trait, and individuals may desire a different level of privacy, which is known as their “privacy concern”. To achieve privacy, the individual has to act in the digital world, taking steps that define their “privacy behavior”. It has been found that there is a gap between people’s privacy concern and their privacy behavior, a phenomenon that is called the “privacy paradox”. In this research, we investigated if the privacy paradox is domain-specific; in other words, does it vary for an individual when that person moves between different domains, for example, when using e-Health services vs. online social networks? A unique metric was developed to estimate the paradox in a way that enables comparisons, and an empirical study in which (n=437) validated participants acted in eight domains. It was found that the domain does indeed affect the magnitude of the privacy paradox. This finding has a profound significance both for understanding the privacy paradox phenomenon and for the process of developing effective means to protect privacy.
Room-Temperature Synthesis of Tubular Hexagonal Boron Nitride under Pressure
Authors: Li Junkai , Jia Donghan , Niu Guoliang , Mu Peiyang , Gou Huiyang
Published: August 2023

Hexagonal boron nitride (h-BN) exhibits interesting optical and mechanical properties, including chemical and thermal stability. Extensive techniques have been applied for the realization of h-BN at high temperatures. Here, we propose a room-temperature preparation of h-BN at high pressure through the compression of ammonium azide and boron powder. The structure and morphology of the obtained h-BN are found to possess tubular-like features, and the selected-area electron diffraction and electron energy-loss spectroscopy support the formation of h-BN. Remarkably, h-BN grows gradually from the surface of boron particles to form a core–shell structure. This tubular morphology of h-BN with a size of 70 nanometers in length and 27 nanometers in width differs from the conventional lamellar h-BN generated with temperature assistance. Our results demonstrate a method for the room-temperature synthesis of tubular h-BN, which shows great promise for the preparation of other nitrides at high pressure and room temperature.