bluuebunny/crossref_metadata_2025 huggingface dataset:
Treatment and Outcomes of Metastatic Non-Small-Cell Lung Cancer Harboring Uncommon EGFR Mutations: Are They Different from Those with Common EGFR Mutations?
Authors: Jung Hyun Ae , Park Sehhoon , Sun Jong-Mu , Lee Se-Hoon , Ahn Jin Seok , Ahn Myung-Ju , Park Keunchil
Published: October 2020

Approximately 10% of the epidermal growth factor receptor (EGFR) mutations in non-small-cell lung cancer (NSCLC) are uncommon EGFR mutations. Although the efficacy of second (2G) or third generation (3G) EGFR tyrosine kinase inhibitors (EGFR-TKIs) in the patients with uncommon EGFR mutation has been proven, further studies are warranted to define the optimal treatment approach for uncommon EGFR mutation-positive NSCLC. This study retrospectively investigated the treatment patterns and outcomes of patients with uncommon EGFR mutation-positive NSCLC from January 2011 to December 2019 at the Samsung Medical Center, Seoul, Korea. During the study, 2121 patients with EGFR mutation-positive NSCLC received first-generation (1G, gefitinib or erlotinib) or 2G EGFR-TKI (afatinib) as the first-line (1L) systemic therapy. Of this, 135 (6.4%) patients harbored uncommon EGFR mutations. Of 135, 54 (40%, 54/135) patients had overlapping mutations with major EGFR mutations. The objective response rate (ORR) for the 1L EGFR-TKI was 63.3%. The median progression-free survivals (PFSs) were 8.6 months (95% CI: 3.8–13.5), 11.7 months (95% CI: 6.6–16.7), 7.7 months (95% CI: 4.9–17.4), and 5.0 months (95% CI: 3.7–6.1) for major uncommon EGFR mutation (G719X, L861Q), compound mutation with major EGFR mutation (Del 19 or EGFR exon 21 p.L858R), other compound mutation, and other uncommon mutations, respectively. The median overall survivals (OSs) were 25.6 months (16.9–34.2), 28.8 (95% CI: 24.4–33.4), 13.5 months (95% CI: 7.4–27.8), and 9.4 months (95% CI: 3.4–10.5) for major uncommon EGFR mutation (G719X), compound mutation with major EGFR mutation (Del 19 or EGFR exon 21 p.L858R), other compound mutation, and other uncommon mutations, respectively. The response rate, median PFS, and OS were 63.3%, 16.3 months (95% CI: 15.6–16.9), and 37.5 months (95% CI: 35.4–39.6) for common EGFR mutation-positive NSCLC. After failing 1L EGFR-TKI, repeated tissue or liquid biopsy were carried out on 44.9% (35/78) of patients with T790M detected in 10/35 (28.6%) patients. With subsequent 3G EGFR-TKI after failing the first-line EGFR-TKI, the ORR and PFS for 3G EGFR-TKI were 80% and 8.9 months (95% CI: 8.0–9.8). These patients showed a median OS of 34.6 months (95% CI: 29.8–39.4). The ORR, PFS and OS were poorer in patients with uncommon (especially other compound and other uncommon mutation) than those with common EGFR mutations. T790M was detected in 28.6% of the uncommon EGFR mutation-positive patients for whom prior 1G/2G EGFR-TKIs failed and underwent repeat biopsy at the time of progression.
Detection and Characterization of Antibacterial Siderophores Secreted by Endophytic Fungi from Cymbidium aloifolium
Authors: Chowdappa Srinivas , Jagannath Shubha , Konappa Narasimhamurthy , Udayashankar Arakere C. , Jogaiah Sudisha
Published: October 2020

Endophytic fungi from orchid plants are reported to secrete secondary metabolites which include bioactive antimicrobial siderophores. In this study endophytic fungi capable of secreting siderophores were isolated from Cymbidium aloifolium, a medicinal orchid plant. The isolated extracellular siderophores from orchidaceous fungi act as chelating agents forming soluble complexes with Fe3+. The 60% endophytic fungi of Cymbidium aloifolium produced hydroxamate siderophore on CAS agar. The highest siderophore percentage was 57% in Penicillium chrysogenum (CAL1), 49% in Aspergillus sydowii (CAR12), 46% in Aspergillus terreus (CAR14) by CAS liquid assay. The optimum culture parameters for siderophore production were 30 °C, pH 6.5, maltose and ammonium nitrate and the highest resulting siderophore content was 73% in P. chrysogenum. The total protein content of solvent-purified siderophore increased four-fold compared with crude filtrate. The percent Fe3+ scavenged was detected by atomic absorption spectra analysis and the highest scavenging value was 83% by P. chrysogenum. Thin layer chromatography of purified P. chrysogenum siderophore showed a wine-colored spot with Rf value of 0.54. HPLC peaks with Rts of 10.5 and 12.5 min were obtained for iron-free and iron-bound P. chrysogenum siderophore, respectively. The iron-free P. chrysogenum siderophore revealed an exact mass-to-charge ratio (m/z) of 400.46 and iron-bound P. chrysogenum siderophore revealed a m/z of 453.35. The solvent-extracted siderophores inhibited the virulent plant pathogens Ralstonia solanacearum, that causes bacterial wilt in groundnut and Xanthomonas oryzae pv. oryzae which causes bacterial blight disease in rice. Thus, bioactive siderophore-producing endophytic P. chrysogenum can be exploited in the form of formulations for development of resistance against other phytopathogens in crop plants.
Evolutionary Study of Disorder in Protein Sequences
Authors: Kastano Kristina , Erdős Gábor , Mier Pablo , Alanis-Lobato Gregorio , Promponas Vasilis J. , Dosztányi Zsuzsanna , Andrade-Navarro Miguel A.
Published: October 2020

Intrinsically disordered proteins (IDPs) contain regions lacking intrinsic globular structure (intrinsically disordered regions, IDRs). IDPs are present across the tree of life, with great variability of IDR type and frequency even between closely related taxa. To investigate the function of IDRs, we evaluated and compared the distribution of disorder content in 10,695 reference proteomes, confirming its high variability and finding certain correlation along the Euteleostomi (bony vertebrates) lineage to number of cell types. We used the comparison of orthologs to study the function of disorder related to increase in cell types, observing that multiple interacting subunits of protein complexes might gain IDRs in evolution, thus stressing the function of IDRs in modulating protein-protein interactions, particularly in the cell nucleus. Interestingly, the conservation of local compositional biases of IDPs follows residue-type specific patterns, with E- and K-rich regions being evolutionarily stable and Q- and A-rich regions being more dynamic. We provide a framework for targeted evolutionary studies of the emergence of IDRs. We believe that, given the large variability of IDR distributions in different species, studies using this evolutionary perspective are required.
Screening for Fabry Disease in Kidney Transplant Recipients: Experience of a Multidisciplinary Team
Authors: Veroux Massimiliano , Monte Ines , Rodolico Margherita , Corona Daniela , Bella Rita , Basile Antonio , Palmucci Stefano , Pistorio Maria , Lanza Giuseppe , De Pasquale Concetta , Veroux Pierfrancesco ,   on behalf of “Multidisciplinary Research Center for the diagnosis and treatment of Fabry Disease and for Organ Transplantation
Published: October 2020

Fabry disease (FD) is a rare cause of end-stage renal disease requiring kidney transplantation. Data on the incidence of unrecognized FD in kidney transplant recipients are scarce and probably underestimated. This study evaluated the incidence of FD in a population of kidney recipients, with a particular focus of the multidisciplinary approach for an early clinical assessment and therapeutic approach. Two hundred sixty-five kidney transplant recipients were screened with a genetic analysis for α-galactosidase A (GLA) mutation, with measurement of α-Gal A enzyme activity and Lyso Gb3 levels. Screening was also extended to relatives of affected patients. Seven patients (2.6%) had a GLA mutation. Two patients had a classic form of FD with Fabry nephropathy. Among the relatives, 15 subjects had a GLA mutation, and two had a Fabry nephropathy. The clinical and diagnostic assessment was completed after a median of 3.2 months, and mean time from diagnosis to treatment was 4.6 months. This study reported a high incidence of unrecognized GLA mutations in kidney transplant recipients. Evaluation and management by a multidisciplinary team allowed for an early diagnosis and treatment, and this would result in a delay in the progression of the disease and, finally, in better long-term outcomes.
Ethanol Dehydrogenation: A Reaction Path Study by Means of Temporal Analysis of Products
Authors: Pasel Joachim , Häusler Johannes , Schmitt Dirk , Valencia Helen , Meledina Maria , Mayer Joachim , Peters Ralf
Published: October 2020

Conventional fossil fuels such as gasoline or diesel should be substituted in the future by environmentally-friendly alternatives in order to reduce emissions in the transport sector and thus mitigate global warming. In this regard, iso-butanol is very promising as its chemical and physical properties are very similar to those of gasoline. Therefore, ongoing research deals with the development of catalytically-supported synthesis routes to iso-butanol, starting from renewably-generated methanol. This research has already revealed that the dehydrogenation of ethanol plays an important role in the reaction sequence from methanol to iso-butanol. To improve the fundamental understanding of the ethanol dehydrogenation step, the Temporal Analysis of Products (TAP) methodology was applied to illuminate that the catalysts used, Pt/C, Ir/C and Cu/C, are very active in ethanol adsorption. H2 and acetaldehyde are formed on the catalyst surfaces, with the latter quickly decomposing into CO and CH4 under the given reaction conditions. Based on the TAP results, this paper proposes a reaction scheme for ethanol dehydrogenation and acetaldehyde decomposition on the respective catalysts. The samples are characterized by means of N2 sorption and Scanning Transmission Electron Microscopy (STEM).
Convection Parametrization and Multi-Nesting Dependence of a Heavy Rainfall Event over Namibia with Weather Research and Forecasting (WRF) Model
Authors: Somses Sieglinde , Bopape Mary-Jane M. , Ndarana Thando , Fridlind Ann , Matsui Toshihisa , Phaduli Elelwani , Limbo Anton , Maikhudumu Shaka , Maisha Robert , Rakate Edward
Published: October 2020

Namibia is considered to be one of the countries that are most vulnerable to climate change due to its generally dry climate and the percentage of its population that rely on subsistence agriculture for their livelihoods. Early-warning systems are an important aspect of adapting to climate change. Weather forecasting relies on the use of numerical weather prediction models and these need to be configured properly. In this study, we investigate the effects of using multi-nests and a convection scheme on the simulation of a heavy rainfall event over the north-western region of Kunene, Namibia. The event, which was associated with a cut-off low system, was short-lived and resulted in over 45 mm of rainfall in one hour. For the multi-nest, a 9 km grid-length parent domain is nested within the Global Forecast System (GFS) simulations, which in turn forces a 3 km grid spacing child domain. A different set of simulations are produced using a single nest of 3 km grid spacing, nested directly inside the GFS data. The simulations are produced with the convection scheme switched on and off. The impact of a single versus multi-nest is found to be small in general, with slight differences in the location of high rainfall intensity. Switching off the convection schemes results in high rainfall intensity and increased detail in the simulations, including when a grid spacing of 9 km is used. Using a grid spacing of 3 km with the convection scheme on, results in a loss of detail in the simulations as well as lower rainfall amounts. The study shows a need for different configurations to be tested before an optimum configuration can be selected for operational forecasting. We recommend further tests with different synoptic forcing and convection schemes to be conducted to identify a suitable configuration for Namibia.
Correction: Vo, T.B.T., et al. Methane Emission Factors from Vietnamese Rice Production: Pooling Data of 36 Field Sites for Meta-analysis. Climate 2020, 8, 74
Authors: Vo Thi Bach Thuong , Wassmann Reiner , Mai Van Trinh , Vu Duong Quynh , Bui Thi Phuong Loan , Vu Thi Hang , Dinh Quang Hieu , Yen Bui Tan , Asch Folkard , Sander Bjoern Ole
Published: October 2020

The authors wish to make the following corrections to this paper [...]
Optimization of Point-Shear Wave Elastography by Skin-to-Liver Distance to Assess Liver Fibrosis in Patients Undergoing Bariatric Surgery
Authors: Giuffrè Mauro , Giuricin Michela , Bonazza Deborah , Rosso Natalia , Giraudi Pablo José , Masutti Flora , Palmucci Stefano , Basile Antonio , Zanconati Fabrizio , de Manzini Nicolò , Tiribelli Claudio , Palmisano Silvia , Crocè Lory Saveria
Published: October 2020

Background: Obesity is a primary limiting factor in liver stiffness measurement (LSM). The impact of obesity has always been evaluated in terms of body mass index (BMI), without studying the effects of skin-to-liver distance (SLD) on LSM. We studied the impact of SLD on LSM in a cohort of obese patients undergoing bariatric surgery and intra-operatory liver biopsy. Materials and Methods: 299 patients underwent LSM by point-shear wave elastography (ElastPQ protocol), with two different ultrasound machines. SLD was measured as the distance between the skin and the liver capsule, perpendicular to where the region of interest (ROI) was positioned. We used the following arbitrary cut-offs: <5.7 kPa, F0–1; 5.7–7.99 kPa, F2; ≥8 kPa, F3–4. Results: We developed two logistic regression models using elastography–histology agreement (EHA) as the dependent variable and SLD as the independent variable. The model based on the second machine showed strongly more performant discriminative and calibration metrics (AIC 38.5, BIC 44.2, Nagelkerke Pseudo-R2 0.894, AUROC 0.90). The SLD cut-off value of 34.5 mm allowed a correct EHA with a sensitivity of 100%, a specificity of 93%, negative predictive value of 100%, positive predictive value of 87%, an accuracy of 96%, and positive likelihood ratio of 3.56. Conclusion: The impact of SLD is machine-dependent and should be taken into consideration when interpreting LSM. We believe that our findings may serve as a reference point for appropriate fibrosis stratification by liver elastography in obese patients.
Intrinsic and Extrinsic Thermodynamics for Stochastic Population Processes with Multi-Level Large-Deviation Structure
Authors: Smith Eric
Published: October 2020

A set of core features is set forth as the essence of a thermodynamic description, which derive from large-deviation properties in systems with hierarchies of timescales, but which are not dependent upon conservation laws or microscopic reversibility in the substrate hosting the process. The most fundamental elements are the concept of a macrostate in relation to the large-deviation entropy, and the decomposition of contributions to irreversibility among interacting subsystems, which is the origin of the dependence on a concept of heat in both classical and stochastic thermodynamics. A natural decomposition that is known to exist, into a relative entropy and a housekeeping entropy rate, is taken here to define respectively the intensive thermodynamics of a system and an extensive thermodynamic vector embedding the system in its context. Both intensive and extensive components are functions of Hartley information of the momentary system stationary state, which is information about the joint effect of system processes on its contribution to irreversibility. Results are derived for stochastic chemical reaction networks, including a Legendre duality for the housekeeping entropy rate to thermodynamically characterize fully-irreversible processes on an equal footing with those at the opposite limit of detailed-balance. The work is meant to encourage development of inherent thermodynamic descriptions for rule-based systems and the living state, which are not conceived as reductive explanations to heat flows.
Very Short-Term Load Forecaster Based on a Neural Network Technique for Smart Grid Control
Authors: Rodríguez Fermín , Martín Fernando , Fontán Luis , Galarza Ainhoa
Published: October 2020

Electrical load forecasting plays a crucial role in the proper scheduling and operation of power systems. To ensure the stability of the electrical network, it is necessary to balance energy generation and demand. Hence, different very short-term load forecast technologies are being designed to improve the efficiency of current control strategies. This paper proposes a new forecaster based on artificial intelligence, specifically on a recurrent neural network topology, trained with a Levenberg–Marquardt learning algorithm. Moreover, a sensitivity analysis was performed for determining the optimal input vector, structure and the optimal database length. In this case, the developed tool provides information about the energy demand for the next 15 min. The accuracy of the forecaster was validated by analysing the typical error metrics of sample days from the training and validation databases. The deviation between actual and predicted demand was lower than 0.5% in 97% of the days analysed during the validation phase. Moreover, while the root mean square error was 0.07 MW, the mean absolute error was 0.05 MW. The results suggest that the forecaster’s accuracy is considered sufficient for installation in smart grids or other power systems and for predicting future energy demand at the chosen sites.
Blockchain-Enabled Demand Response Scheme with Individualized Incentive Pricing Mode
Authors: Guo Zishan , Ji Zhenya , Wang Qi
Published: October 2020

Demand response (DR) can offer a wide range of advantages for electrical systems by facilitating the interaction and balance between supply and demand. However, DR always requires a central agent, giving rise to issues of security and trust. Besides this, differences in user response cost characteristics are not taken into consideration during incentive pricing, which would affect the equal participation of users in DR and increase the costs borne by the electricity retail company. In this paper, a blockchain-enabled DR scheme with an individualized incentive pricing mode is proposed. First, a blockchain-enabled DR framework is proposed to promote the secure implementation of DR. Next, a dual-incentive mechanism is designed to successfully implement the blockchain to DR, which consists of a profit-based and a contribution-based model. An individualized incentive pricing mode is adopted in the profit-based model to decrease the imbalance in response frequency of users and reduce the costs borne by the electricity retail company. Then, the Stackelberg game model is constructed and Differential Evolution (DE) is used to produce equilibrium optimal individualized incentive prices. Finally, case studies are conducted. The results demonstrate that the proposed scheme can reduce the cost borne by the electricity retail company and decrease the imbalance among users in response frequency.
Specific Energy Consumption of a Belt Conveyor System in a Continuous Surface Mine
Authors: Kawalec Witold , Suchorab Natalia , Konieczna-Fuławka Martyna , Król Robert
Published: October 2020

Belt conveyor (BC) transportation systems are considered to be the most effective for handling large volumes of bulk material. With regards to the rules of sustainable development, the improvement of belt conveyor technology is, in many cases, focused on environmental issues, which include the idea of energy usage optimization. The key issue in an energy-efficient transportation system is reducing the value of specific energy consumption (SEC) by increasing conveyor capacity whilst decreasing belt conveyor motion resistance. The main idea of this paper is to conduct an analysis of the modernization of existing belt conveyor transportation systems operated in open-pit lignite mines, in order to achieve relatively small electric energy consumption for a considered transportation task. The first part of the paper investigates the relationship between a conveyor’s SEC and material flow rate for various conveyor design parameters. Then, based on multi-parameter simulations, an analysis of electric energy consumption for a belt conveyor transportation system is carried out. Finally, an energy-saving, environmentally friendly solution is presented.
Cooperative Control for Multi-Module Charging Systems of Ultracapacitors
Authors: Zhang Xiaoyong , Lei Jiaxuan , Li Heng , Liao Hongtao , Peng Jun
Published: October 2020

Ultracapacitors have recently received great attention for energy storage due to their small pollution, high power density, and long lifetime. In many applications, ultracapacitors need to be charged with a high current, where a multi-module charging system is typically adopted. Although the classical decentralized control method can control the charging process of ultracapacitors, there exists a problem that the charging current may be imbalanced among charging modules. In this paper, a cooperative cascade charging method is proposed for the multi-module charging system to reduce the current imbalance among charging modules. First, the state-space averaging method and graph theory are used to model the multiple-module charging system. Second, an effective cooperative cascade control is proposed, where the outer voltage loop stabilizes the output voltage to the desired voltage and the inner current loop guarantees the current of each charger to follow the target current. The block diagram is used to establish the closed-loop model of the charging system. In order to evaluate the proposed charging method, a laboratory prototype was established. Compared with the classical decentralized method, this method can effectively suppress the current imbalance, which is proved by simulation and experimental results.
Heat Transfer Enhancement of Plate-Fin Heat Sinks with Different Types of Winglet Vortex Generators
Authors: Shyu Jin-Cherng , Jheng Jhao-Siang
Published: October 2020

Because the delta winglet in common-flow-down configuration has been recognized as an excellent type of vortex generators (VGs), this study aims to experimentally and numerically investigate the thermo-hydraulic performance of four different forms of winglet VGs featuring sweptback delta winglets in the channel flow in the range 200 < Re < 1000. Both Nusselt number and friction factor of plate-fin heat sinks having different forms of winglets, including delta winglet pair (DWP), rectangular winglet pair (RWP), swept delta winglet pair (SDWP), and swept trapezoid winglet pair (STWP), were measured in a standard wind tunnel without bypass in this study. Four rows of winglets with in-line arrangement were punched on each 10-mm-long, 0.2-mm-thick copper plate, and a total of 16 pieces of copper plates with spacing of 2 mm were fastened together to achieve the heat sink. The projected area, longitudinal and winglet tip spacing, height and angle of attack of those winglets were fixed. Besides that, three-dimensional numerical simulation was also performed in order to investigate the temperature and fluid flow over the plate-fin. The results showed that the longitudinal, common-flow-down vortices generated by the VGs augmented the heat transfer and pressure drop of the heat sink. At airflow velocity of 5 m/s, the heat transfer coefficient and pressure drop of plain plate-fin heat sink were 50.8 W/m2·K and 18 Pa, respectively, while the heat transfer coefficient and the pressure drop of heat sink having SDWP were 70.4 W/m2·K and 36 Pa, respectively. It was found that SDWP produced the highest thermal enhancement factor (TEF) of 1.28 at Re = 1000, followed by both RWP and STWP of similar TEF in the range 200 < Re < 1000. The TEF of DWP was the lowest and it was rapidly increased with the increase of airflow velocity.
Heat-Dissipation Performance of Nanocomposite Phase-Change Materials in a Twin-Heat-Source System
Authors: Li Yanxin , Wang Jin , Yang Li , Sundén Bengt
Published: October 2020

In this paper, pure paraffin was mixed with CuO (high thermal conductivity) and Span-80 (as a dispersant). The CuO/paraffin nanocomposite phase-change materials (PCMs) were synthesized with mass fractions of 0.3%, 0.6%, and 1.2%, by a two-step method. Heat-transfer characteristics of the heat-pipe–PCMs module and effects of fan power and heating power on the performance of the cooling module in a twin-heat-source system were studied. For two heat sources under 10 W–10 W (heat source 1 with a power of 10 W and heat source 2 with a power of 10 W), the paraffin wax decreases the evaporator temperature by 14.4%, compared with cases without PCMs.
A Built Heritage Information System Based on Point Cloud Data: HIS-PC
Authors: Poux Florent , Billen Roland , Kasprzyk Jean-Paul , Lefebvre Pierre-Henri , Hallot Pierre
Published: October 2020

The digital management of an archaeological site requires to store, organise, access and represent all the information that is collected on the field. Heritage building information modelling, archaeological or heritage information systems now tend to propose a common framework where all the materials are managed from a central database and visualised through a 3D representation. In this research, we offer the development of a built heritage information system prototype based on a high-resolution 3D point cloud data set. The particularity of the approach is to consider a user-centred development methodology while avoiding meshing/down-sampling operations. The proposed system is initiated by a close collaboration between multi-modal users (managers, visitors, curators) and a development team (designers, developers, architects). The developed heritage information system permits the management of spatial and temporal information, including a wide range of semantics using relational along with NoSQL databases. The semantics used to describe the artifacts are subject to conceptual modelling. Finally, the system proposes a bi-directional communication with a 3D interface able to stream massive point clouds, which is a big step forward to provide a comprehensive site representation for stakeholders while minimising modelling costs.
Evaluation of the Space Syntax Measures Affecting Pedestrian Density through Ordinal Logistic Regression Analysis
Authors: Hacar Özge , Gülgen Fatih , Bilgi Serdar
Published: October 2020

This paper examines the relationship between pedestrian density and space syntax measures in a university campus using ordinal logistic regression analysis. The pedestrian density assumed as the dependent variable of regression analysis was categorised in low, medium, and high classes by using Jenks natural break classification. The data elements of groups were derived from pedestrian counts performed in 22 gates 132 times. The counting period grouped in nominal categories was assumed as an independent variable. Another independent was one of the 15 derived measures of axial analysis and visual graphic analysis. The statistically significant model results indicated that the integration of axial analysis was the most reasonable measure that explained the pedestrian density. Then, the changes in integration values of current and master plan datasets were analysed using paired sample t-test. The calculated p-value of t-test proved that the master plan would change the campus morphology for pedestrians.
Role of the Triceps Surae Muscles in Patients Undergoing Anterior Cruciate Ligament Reconstruction: A Matched Case-Control Study
Authors: Rhim Hye Chang , Lee Jin Hyuck , Han Seung-Beom , Shin Kyun-Ho , Suh Dong Won , Jang Ki-Mo
Published: October 2020

A limited number of studies has investigated the gastrocnemius and soleus in patients undergoing anterior cruciate ligament reconstruction (ACLR). This study investigated the muscle strength (Nm kg−1 × 100) and reaction time (acceleration time (AT), milliseconds) of thigh and calf muscles in patients undergoing ACLR. Thirty-two patients with ACLR and 32 normal control subjects were included. One year postoperatively, the strength of thigh muscles was significantly reduced after ACLR compared with that of controls (hamstring: 80 ± 31.3 vs. 142 ± 26.4, p < 0.001, quadriceps: 159 ± 63.7 vs. 238 ± 35.3, p < 0.001). However, the strength of calf muscles was not significantly different compared with that of controls (gastrocnemius: 77 ± 22.9 vs. 81 ± 22.5, p = 0.425, soleus: 54 ± 15.9 vs. 47 ± 16.1, p = 0.109). The AT of calf muscles was significantly faster after ACLR than that of controls (gastrocnemius: 26 ± 9.8 vs. 31 ± 9, p = 0.030, soleus: 18 ± 6.7 vs. 22 ± 8.5, p = 0.026). The AT of thigh muscles was significantly elongated after ACLR than that of controls (hamstring: 72 ± 18 vs. 55 ± 12.4, p < 0.001, quadriceps: 63 ± 17.6 vs. 47 ± 17, p < 0.000). The strength of thigh muscles was reduced, and the ATs of thigh muscles were slower one year after ACLR. However, the AT of the triceps surae was faster than that of controls. This may implicate a compensatory mechanism of the triceps surae for the weakness and delayed activation in hamstring and quadriceps muscles.
Biopsy-Controlled Non-Invasive Quantification of Collagen Type VI in Kidney Transplant Recipients: A Post-Hoc Analysis of the MECANO Trial
Authors: Yepes-Calderón Manuela , Sotomayor Camilo G. , Rasmussen Daniel Guldager Kring , Hijmans Ryanne S. , te Velde-Keyzer Charlotte A. , van Londen Marco , van Dijk Marja , Diepstra Arjan , Berger Stefan P. , Karsdal Morten Asser , Bemelman Frederike J. , de Fijter Johan W. , Kers Jesper , Florquin Sandrine , Genovese Federica , Bakker Stephan J. L. , Sanders Jan-Stephan , Van Den Born Jacob
Published: October 2020

The PRO-C6 assay, a reflection of collagen type VI synthesis, has been proposed as a non-invasive early biomarker of kidney fibrosis. We aimed to investigate cross-sectional and longitudinal associations between plasma and urine PRO-C6 and proven histological changes after kidney transplantation. The current study is a post-hoc analysis of 94 participants of the MECANO trial, a 24-month prospective, multicenter, open-label, randomized, controlled trial aimed at comparing everolimus-based vs. cyclosporine-based immunosuppression. PRO-C6 was measured in plasma and urine samples collected 6 and 24 months post-transplantation. Fibrosis was evaluated in biopsies collected at the same time points by Banff interstitial fibrosis/tubular atrophy (IF/TA) scoring and collagen staining (Picro Sirius Red; PSR); inflammation was evaluated by the tubulo-interstitial inflammation score (ti-score). Linear regression analyses were performed. Six-month plasma PRO-C6 was cross-sectionally associated with IF/TA score (Std. β = 0.34), and prospectively with 24-month IF/TA score and ti-score (Std. β = 0.24 and 0.23, respectively) (p < 0.05 for all). No significant associations were found between urine PRO-C6 and any of the biopsy findings. Fibrotic changes and urine PRO-C6 behaved differentially over time according to immunosuppressive therapy. These results are a first step towards non-invasive fibrosis detection after kidney transplantation by means of collagen VI synthesis measurement, and further research is required.
COVID-19 Triage and Test Center: Safety, Feasibility, and Outcomes of Low-Threshold Testing
Authors: Mansella Gregory , Rueegg Marco , Widmer Andreas F. , Tschudin-Sutter Sarah , Battegay Manuel , Hoff Julia , Søgaard Kirstine K. , Egli Adrian , Stieltjes Bram , Leuzinger Karoline , Hirsch Hans H. , Meienberg Andrea , Burkard Thilo , Mayr Michael , Bingisser Roland , Nickel Christian H.
Published: October 2020

This prospective observational study evaluated the safety and feasibility of a low threshold testing process in a Triage and Test Center (TTC) during the early course of the coronavirus disease 19 (COVID-19) pandemic. In addition, we aimed to identify clinical predictors for a positive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) swab result. Patients underwent informal triage, standardized history taking, and physician evaluation, only where indicated. Patients were observed for 30 days. Safety was the primary outcome and was defined as a COVID-19-related 30 day re-presentation rate <5% and mortality rate <1% in patients presenting to the TTC. Feasibility was defined as an overruling of informal triage <5%. Among 4815 presentations, 572 (11.9%) were tested positive for SARS-CoV-2, and 4774 were discharged. Mortality at 30-days was 0.04% (2 patients, one of which related to COVID-19). Fever (OR 2.03 [95% CI 1.70;2.42]), myalgia (OR 1.94 [1.63;2.31]), chills (OR 1.77 [1.44;2.16]), headache (OR 1.61 [1.34;1.94]), cough (OR 1.50 [1.24;1.83]), weakness (OR 1.46 [1.21;1.76]), and confusion (OR 1.39 [1.06;1.80]) were associated with test positivity. Re-presentation rate was 8% overall and 1.4% in COVID-19 related re-presentation (69 of 4774). The overruling rate of informal triage was 1.5%. According to our study, a low-threshold testing process in a TTC appeared to be safe (low re-presentation and low mortality) and is feasible (low overruling of informal triage). A COVID-19 diagnosis based on clinical parameters only does not appear possible.
Marine Snow Aggregates are Enriched in Polycyclic Aromatic Hydrocarbons (PAHs) in Oil Contaminated Waters: Insights from a Mesocosm Study
Authors: Bacosa Hernando P. , Kamalanathan Manoj , Cullen Joshua , Shi Dawei , Xu Chen , Schwehr Kathleen A. , Hala David , Wade Terry L. , Knap Anthony H. , Santschi Peter H. , Quigg Antonietta
Published: October 2020

Marine snow was implicated in the transport of oil to the seafloor during the Deepwater Horizon oil spill, but the exact processes remain controversial. In this study, we investigated the concentrations and distributions of the 16 USEPA priority polycyclic aromatic hydrocarbons (PAHs) in marine snow aggregates collected during a mesocosm experiment. Seawater only, oil in a water accommodated fraction (WAF), and Corexit-enhanced WAF (DCEWAF) were incubated for 16 d. Both WAF and DCEWAF aggregates were enriched in heavy molecular weight PAHs but depleted in naphthalene. DCEWAF aggregates had 2.6 times more total 16 PAHs than the WAF (20.5 vs. 7.8 µg/g). Aggregates in the WAF and DCEWAF incorporated 4.4% and 19.3%, respectively of the total PAHs in the mesocosm tanks. Our results revealed that marine snow sorbed and scavenged heavy molecular weight PAHs in the water column and the application of Corexit enhanced the incorporation of PAHs into the sinking aggregates.
Strategy for Application of Support Object for Fall Prevention in the Elderly Based on Balance Recovery Characteristics
Authors: Matsuda Soichiro , Takeda Yukio
Published: October 2020

This research proposes a strategy for applying support objects—equipment, tools, and even furniture/environment from which humans can receive reaction force through their hands—for fall prevention in the elderly. This paper presents an assessment example of support objects based on balance recovery characteristics and a discussion regarding their application according to the assessment results. The balance recovery characteristics depend on the direction in which reaction force can be obtained based on the shape of the support object and direction in which the force is easily exerted on the hand. Evaluation indices for assessing the height and shape of nonportable support objects and determining a position of the tip on the ground of a cane, a typical portable support object, in the anterior direction are briefly introduced based on the authors’ previous works. The strategy for the application of support objects utilizing the evaluation indices is proposed; better use of support objects, their locations, new-shaped ones, and support devices with a new design concept are discussed and introduced based on the values of the calculated indices according to the type/usage of the support objects.
Repdigits as Product of Fibonacci and Tribonacci Numbers
Authors: Bednařík Dušan , Trojovská Eva
Published: October 2020

In this paper, we study the problem of the explicit intersection of two sequences. More specifically, we find all repdigits (i.e., numbers with only one repeated digit in its decimal expansion) which can be written as the product of a Fibonacci by a Tribonacci number (both with the same indexes). To work on this problem, our approach is to combine lower bounds from the Baker’s theory with reduction methods (based on the theory of continued fractions) due to Dujella and Pethö.
An Integrated SWARA-WASPAS Group Decision Making Framework to Evaluate Smart Card Systems for Public Transportation
Authors: Baç Uğur
Published: October 2020

Recent technological developments affect daily life as much as they affect the industries. As part of these developments, automation and smart systems are important part of everyday life. Smart card systems are one of the well-known types of smart automation technology being used by the majority of the population in public transportation in most developed countries. Even though automated fare payment systems have been widely integrated into public transportation in developed countries, integration of smart card systems is still under consideration in most developing countries. The aim of this study is to propose a framework to evaluate different smart card systems to determine the best one and additionally validate their benefits when compared with the traditional fare payment system. For this purpose, an integrated multi-criteria decision making (MCDM) framework is used that combines two recent and popular methodologies together. The proposed methodology employs Stepwise Weight Assessment Ratio Analysis (SWARA) method for determination of criteria weights in the decision model and the Weighted Additive Sum Product Assessment (WASPAS) method for comparison of alternatives. Research results revealed that all smart card systems show improvements under performance, reliability, and user satisfaction related criteria. However, traditional fare payment systems are found to be safer under consideration of personal data protection. Findings of this study can be used to select the best smart card system and as a guide for deciding on areas of improvement during the implementation phase to ensure higher user satisfaction.
State of the Art in Exocrine Pancreatic Insufficiency
Authors: Diéguez-Castillo Carmelo , Jiménez-Luna Cristina , Prados Jose , Martín-Ruiz José Luis , Caba Octavio
Published: October 2020

Exocrine pancreatic insufficiency (EPI) is defined as the maldigestion of foods due to inadequate pancreatic secretion, which can be caused by alterations in its stimulation, production, transport, or interaction with nutrients at duodenal level. The most frequent causes are chronic pancreatitis in adults and cystic fibrosis in children. The prevalence of EPI is high, varying according to its etiology, but it is considered to be underdiagnosed and undertreated. Its importance lies in the quality of life impairment that results from the malabsorption and malnutrition and in the increased morbidity and mortality, being associated with osteoporosis and cardiovascular events. The diagnosis is based on a set of symptoms, indicators of malnutrition, and an indirect non-invasive test in at-risk patients. The treatment of choice combines non-restrictive dietary measures with pancreatic enzyme replacement therapy to correct the associated symptoms and improve the nutritional status of patients. Non-responders require the adjustment of pancreatic enzyme therapy, the association of proton pump inhibitors, and/or the evaluation of alternative diagnoses such as bacterial overgrowth. This review offers an in-depth overview of EPI in order to support the proper management of this entity based on updated and integrated knowledge of its etiopathogenesis, prevalence, diagnosis, and treatment.
Left Ventricular Diastolic and Systolic Functions in Patients with Hypothyroidism
Authors: Tafarshiku Rina , Henein Michael Y. , Berisha-Muharremi Venera , Bytyçi Ibadete , Ibrahimi Pranvera , Poniku Afrim , Elezi Shpend , Bajraktari Gani
Published: October 2020

Background and objectives: Long standing hypothyroidism may impair myocardial relaxation, but its effect on systolic myocardial function is still controversial. The aim of this study was to investigate left ventricular (LV) systolic and diastolic function in patients with hypothyroidism. Materials and Methods: This study included 81 (age 42 ± 13 years, 92% female) patients with hypothyroidism, and 22 age and gender matched controls. All subjects underwent a detailed clinical examination followed by a complete biochemical blood analysis including thyroid function assessment and anthropometric parameters measurements. LV function was assessed by 2-dimensional, M-mode and Tissue-Doppler Doppler echocardiographic examination performed in the same day. Results: Patients had lower waist/hip ratio (p< 0.001), higher urea level (p = 0.002), and lower white blood cells (p = 0.011), compared with controls. All other clinical, biochemical, and anthropometric data did not differ between the two groups. Patients had impaired LV diastolic function (lower E wave [p< 0.001], higher A wave [p = 0.028], lower E/A ratio [p< 0.001], longer E wave deceleration time [p = 0.01], and higher E/e’ ratio [p< 0.001]), compared with controls. Although LV global systolic function did not differ between groups, LV longitudinal systolic function was compromised in patients (lateral mitral annular plane systolic excursion—MAPSE [p = 0.005], as were lateral and septal s’ [p< 0.001 for both]). Conclusions: In patients with hypothyroidism, in addition to compromised LV diastolic function, LV longitudinal systolic function is also impaired compared to healthy subjects of the same age and gender. These findings suggest significant subendocardial function impairment, reflecting potentially micro-circulation disease that requires optimum management.
Effects of Storage Temperature and pH on the Antifungal Effects of Commercial Oral Moisturizers against Candida albicans and Candida glabrata
Authors: Murakami Mamoru , Harada Kae , Nishi Yasuhiro , Shimizu Takaharu , Motoyama Sara , Nishimura Masahiro
Published: October 2020

Background and objectives: Oral moisturizers have been used to treat dry mouth. This study aimed to investigate the effects of storage temperature and pH on the antifungal effects of oral moisturizers against Candida albicans and Candida glabrata. Materials and Methods: Thirty-one oral moisturizers and amphotericin B (AMPH-B) were stored at 25 and 37 °C for 1 week. Subsequently, they were added to cylindrical holes in 50% trypticase soy agar plates inoculated with C. albicans and C. glabrata (107 cells/ml). The antifungal effects were evaluated based on the sizes of the growth-inhibitory zones formed. Two-way analysis of variance was used to determine the effects of storage temperature and pH on the growth-inhibitory zones. Results: Significant differences in the effects of storage temperature and pH of the moisturizers were observed against C. albicans and C. glabrata. The growth-inhibitory zones of samples stored at 37 °C and with neutral pH were significantly larger than those stored at 25 °C and with acidic pH, respectively. The sizes of the zones formed by most of the oral moisturizers were larger than those formed by AMPH-B (concentration, 0.63 µg/ml). Conclusion: The antifungal effects of oral moisturizers against C. albicans and C. glabrata were affected by their storage temperature and pH.
Comparison of Nucleic Acid Extraction Methods for a Viral Metagenomics Analysis of Respiratory Viruses
Authors: Sabatier Marina , Bal Antonin , Destras Grégory , Regue Hadrien , Quéromès Grégory , Cheynet Valérie , Lina Bruno , Bardel Claire , Brengel-Pesce Karen , Navratil Vincent , Josset Laurence
Published: October 2020

Viral metagenomics next-generation sequencing (mNGS) is increasingly being used to characterize the human virome. The impact of viral nucleic extraction on virome profiling has been poorly studied. Here, we aimed to compare the sensitivity and sample and reagent contamination of three extraction methods used for viral mNGS: two automated platforms (eMAG; MagNA Pure 24, MP24) and the manual QIAamp Viral RNA Mini Kit (QIAamp). Clinical respiratory samples (positive for Respiratory Syncytial Virus or Herpes Simplex Virus), one mock sample (including five viruses isolated from respiratory samples), and a no-template control (NTC) were extracted and processed through an mNGS workflow. QIAamp yielded a lower proportion of viral reads for both clinical and mock samples. The sample cross-contamination was higher when using MP24, with up to 36.09% of the viral reads mapping to mock viruses in the NTC (vs. 1.53% and 1.45% for eMAG and QIAamp, respectively). The highest number of viral reads mapping to bacteriophages in the NTC was found with QIAamp, suggesting reagent contamination. Our results highlight the importance of the extraction method choice for accurate virome characterization.
Fused Omics Data Models Reveal Gut Microbiome Signatures Specific of Inactive Stage of Juvenile Idiopathic Arthritis in Pediatric Patients
Authors: Vernocchi Pamela , Marini Federico , Capuani Giorgio , Tomassini Alberta , Conta Giorgia , Del Chierico Federica , Malattia Clara , De Benedetti Fabrizio , Martini Alberto , Dallapiccola Bruno , van Dijkhuizen E. H. Pieter , Miccheli Alfredo , Putignani Lorenza
Published: October 2020

Juvenile idiopathic arthritis (JIA) is the most common rheumatic disease in children. Herein, we evaluated the relationship between the gut microbiome (GM) and disease phenotype by an integrated omics fused approach. In a multicenter, observational cohort study, stools from Italian JIA patients were collected at baseline, active, and inactive disease stages, and their GM compared to healthy controls (CTRLs). The microbiota metabolome was analyzed to detect volatile- and non-volatile organic compounds (VOCs); the data were fused with operational taxonomic units (OTUs) from 16S RNA targeted-metagenomics and classified by chemometric models. Non-VOCs did not characterize JIA patients nor JIA activity stages compared to CTRLs. The core of VOCs, (Ethanol, Methyl-isobutyl-ketone, 2,6-Dimethyl-4-heptanone and Phenol) characterized patients at baseline and inactive disease stages, while the OTUs represented by Ruminococcaceae, Lachnospiraceae and Clostridiacea discriminated between JIA inactive stage and CTRLs. No differences were highlighted amongst JIA activity stages. Finally, the fused data discriminated inactive and baseline stages versus CTRLs, based on the contribution of the invariant core of VOCs while Ruminococcaceae concurred for the inactive stage versus CTRLs comparison. In conclusion, the GM signatures enabled to distinguish the inactive disease stage from CTRLs.
Metal Oxide Nanoparticles Against Bacterial Biofilms: Perspectives and Limitations
Authors: Shkodenko Liubov , Kassirov Ilia , Koshel Elena
Published: October 2020

At present, there is an urgent need in medicine and industry to develop new approaches to eliminate bacterial biofilms. Considering the low efficiency of classical approaches to biofilm eradication and the growing problem of antibiotic resistance, the introduction of nanomaterials may be a promising solution. Outstanding antimicrobial properties have been demonstrated by nanoparticles (NPs) of metal oxides and their nanocomposites. The review presents a comparative analysis of antibiofilm properties of various metal oxide NPs (primarily, CuO, Fe3O4, TiO2, ZnO, MgO, and Al2O3 NPs) and nanocomposites, as well as mechanisms of their effect on plankton bacteria cells and biofilms. The potential mutagenicity of metal oxide NPs and safety problems of their wide application are also discussed.