bluuebunny/crossref_metadata_2025 huggingface dataset:
Combined Flexural and Shear Strengthening of RC T-Beams with FRP and TRM: Experimental Study and Parametric Finite Element Analyses
Authors: Pohoryles Daniel A. , Melo Jose , Rossetto Tiziana
Published: November 2021

Due to inadequacies of reinforcement design in older structures and changes in building codes, but also the change of building use in existing structures, reinforced concrete (RC) beams often require upgrading during building renovation. The combined shear and flexural strengthening with composite materials, fibre-reinforced polymer sheets (FRP) and textile reinforced mortars (TRM), is assessed in this study. An experimental campaign on twelve half-scale retrofitted RC beams is presented, looking at various parameters of interest, including the effect of the steel reinforcement ratio on the retrofit effectiveness, the amount of composite material used for strengthening and the effect of the shear span, as well as the difference in effectiveness of FRP and TRM in strengthening RC beams. Significant effects on the shear capacity of composite retrofitted beams are observed for all studied parameters. The experimental study is used as a basis for developing a detailed finite element (FE) model for RC beams strengthened with FRP. The results of the FE model are compared to the experimental results and used to design a parametric study to further study the effect of the investigated parameters on the retrofit effectiveness.
Numerical Investigation of Bioaerosol Transport in a Compact Lavatory
Authors: Wan Jingyuan , Wei Jianjian , Lin Yingtien , Zhang Tengfei (Tim)
Published: November 2021

The lavatory is a fertile area for the transmission of infectious disease through bioaerosols between its users. In this study, we built a generic compact lavatory model with a vacuum toilet, and computational fluid dynamics (CFD) is used to evaluate the effects of ventilation and user behaviors on the airflow patterns, and the resulting fates of bioaerosols. Fecal aerosols are readily released into the lavatory during toilet flush. Their concentration rapidly decays in the first 20 s after flushing by deposition or dilution. It takes about 315 s to 348 s for fine bioaerosols (<10 µm in diameter) to decrease to 5% of the initial concentration, while it takes 50 and 100 µm bioaerosols approximately 11 and <1 s, respectively, to completely deposit. The most contaminated surfaces by aerosol deposition include the toilet seat, the bowl, and the nearby walls. The 10 µm aerosols tend to deposit on horizontal surfaces, while the 50 and 100 µm bioaerosols almost always deposit on the bowl. In the presence of a standing thermal manikin, the rising thermal plume alters the flow field and more bioaerosols are carried out from the toilet; a large fraction of aerosols deposit on the manikin’s legs. The respiratory droplets generated by a seated coughing manikin tend to deposit on the floor, legs, and feet of the manikin. In summary, this study reveals the bioaerosol dilution time and the easily contaminated surfaces in a compact lavatory, which will aid the development of control measures against infectious diseases.
Investigation of Filtration Phenomena of Air Pollutants on Cathode Air Filters for PEM Fuel Cells
Authors: Özyalcin Can , Mauermann Peter , Dirkes Steffen , Thiele Paul , Sterlepper Stefan , Pischinger Stefan
Published: November 2021

Filtration of cathode air is one of the challenges in operating proton-exchange membrane (PEM) fuel cells. The poisoning with air contaminants can lead to rapid performance degradation and initiate an aging process of the fuel cell. Various commercially available cathode filters are being tested in a laboratory gas test bench within the research project X-EMU (03B10502B and 03B10502B2). A literature review of harmful gas contaminants in the air used for the oxygen reduction reaction (ORR) on the cathode side was conducted. Experimental investigations took place at 40 °C with synthetic humid air containing low concentration contaminants such as ammonia, nitrogen dioxide, carbon monoxide, sulfur dioxide, hydrogen sulfide, and toluene. Test durations varied from 3 to 24 h depending on the filtration efficiency. Each gas contaminant showed different reactions with the investigated filters. The filters did not let sulfur-containing components pass. However, carbon monoxide could not be filtrated by any of the tested filters. The filtration of nitrogen oxides was not efficient for all tested filters, while additional filter materials were essential for a successful filtration of ammonia. Comparative results lead to a discussion of possible effects on a fuel cell with an outlook on optimization of the filtration behavior.
An Experimental Study on the Biological Fixation and Effective Use of Carbon Using Biogas and Bacterial Community Dominated by Methanotrophs, Methanol-Oxidizing Bacteria, and Ammonia-Oxidizing Bacteria
Authors: Kim I-Tae , Ahn Kwang-Ho , Lee Ye-Eun , Jeong Yoonah , Park Jae-Roh , Shin Dong-Chul , Jung Jinhong
Published: November 2021

This study used biogas from a wastewater treatment plant and bacterial community where methanotrophs, Methylophilus, and ammonia-oxidizing bacteria clusters coexisted to propose an effective method for biological carbon fixation and nitrogen removal in wastewater treatment for carbon capture, utilization, and storage (CCUS). Biogas from wastewater treatment plant was provided, instead of purified CH4, to provide operation and maintenance conditions of bio-catalyst reaction for efficient biological carbon fixation by bacterial community using methane and CO2. This study assessed the conditions to induce type X methanotrophs that can use CO2 as a carbon source, as dominant species in the bacterial community and continuously and effectively supply reducing equivalents required for the conversion of CO2 to methanol within the system. Herein, the results of inducing efficient co-existence of methanotrophs, Methylophilus, and ammonia-oxidizing bacteria cluster in the bacterial community were shown.
Novel Galectin-3 Roles in Neurogenesis, Inflammation and Neurological Diseases
Authors: Soares Luana C. , Al-Dalahmah Osama , Hillis James , Young Christopher C. , Asbed Isaiah , Sakaguchi Masanori , O’Neill Eric , Szele Francis G.
Published: November 2021

Galectin-3 (Gal-3) is an evolutionarily conserved and multifunctional protein that drives inflammation in disease. Gal-3’s role in the central nervous system has been less studied than in the immune system. However, recent studies show it exacerbates Alzheimer’s disease and is upregulated in a large variety of brain injuries, while loss of Gal-3 function can diminish symptoms of neurodegenerative diseases such as Alzheimer’s. Several novel molecular pathways for Gal-3 were recently uncovered. It is a natural ligand for TREM2 (triggering receptor expressed on myeloid cells), TLR4 (Toll-like receptor 4), and IR (insulin receptor). Gal-3 regulates a number of pathways including stimulation of bone morphogenetic protein (BMP) signaling and modulating Wnt signalling in a context-dependent manner. Gal-3 typically acts in pathology but is now known to affect subventricular zone (SVZ) neurogenesis and gliogenesis in the healthy brain. Despite its myriad interactors, Gal-3 has surprisingly specific and important functions in regulating SVZ neurogenesis in disease. Gal-1, a similar lectin often co-expressed with Gal-3, also has profound effects on brain pathology and adult neurogenesis. Remarkably, Gal-3’s carbohydrate recognition domain bears structural similarity to the SARS-CoV-2 virus spike protein necessary for cell entry. Gal-3 can be targeted pharmacologically and is a valid target for several diseases involving brain inflammation. The wealth of molecular pathways now known further suggest its modulation could be therapeutically useful.
A Cell-Based Platform for the Investigation of Immunoproteasome Subunit β5i Expression and Biology of β5i-Containing Proteasomes
Authors: Burov Alexander , Funikov Sergei , Vagapova Elmira , Dalina Alexandra , Rezvykh Alexander , Shyrokova Elena , Lebedev Timofey , Grigorieva Ekaterina , Popenko Vladimir , Leonova Olga , Spasskaya Daria , Spirin Pavel , Prassolov Vladimir , Karpov Vadim , Morozov Alexey
Published: November 2021

The degradation of most intracellular proteins is a dynamic and tightly regulated process performed by proteasomes. To date, different forms of proteasomes have been identified. Currently the role of non-constitutive proteasomes (immunoproteasomes (iPs) and intermediate proteasomes (intPs)) has attracted special attention. Here, using a CRISPR-Cas9 nickase technology, four cell lines: histiocytic lymphoma, colorectal adenocarcinoma, cervix adenocarcinoma, and hepatocarcinoma were modified to express proteasomes with mCherry-tagged β5i subunit, which is a catalytic subunit of iPs and intPs. Importantly, the expression of the chimeric gene in modified cells is under the control of endogenous regulatory mechanisms and is increased following IFN-γ and/or TNF-α stimulation. Fluorescent proteasomes retain catalytic activity and are distributed within the nucleus and cytoplasm. RNAseq reveals marginal differences in gene expression profiles between the modified and wild-type cell lines. Predominant metabolic pathways and patterns of expressed receptors were identified for each cell line. Using established cell lines, we demonstrated that anti-cancer drugs Ruxolitinib, Vincristine and Gefitinib stimulated the expression of β5i-containing proteasomes, which might affect disease prognosis. Taken together, obtained cell lines can be used as a platform for real-time studies of immunoproteasome gene expression, localization of iPs and intPs, interaction of non-constitutive proteasomes with other proteins, proteasome trafficking and many other aspects of proteasome biology in living cells. Moreover, the established platform might be especially useful for fast and large-scale experiments intended to evaluate the effects of different conditions including treatment with various drugs and compounds on the proteasome pool.
Cell-to-Cell Interactions Mediating Functional Recovery after Stroke
Authors: Alia Claudia , Cangi Daniele , Massa Verediana , Salluzzo Marco , Vignozzi Livia , Caleo Matteo , Spalletti Cristina
Published: November 2021

Ischemic damage in brain tissue triggers a cascade of molecular and structural plastic changes, thus influencing a wide range of cell-to-cell interactions. Understanding and manipulating this scenario of intercellular connections is the Holy Grail for post-stroke neurorehabilitation. Here, we discuss the main findings in the literature related to post-stroke alterations in cell-to-cell interactions, which may be either detrimental or supportive for functional recovery. We consider both neural and non-neural cells, starting from astrocytes and reactive astrogliosis and moving to the roles of the oligodendrocytes in the support of vulnerable neurons and sprouting inhibition. We discuss the controversial role of microglia in neural inflammation after injury and we conclude with the description of post-stroke alterations in pyramidal and GABAergic cells interactions. For all of these sections, we review not only the spontaneous evolution in cellular interactions after ischemic injury, but also the experimental strategies which have targeted these interactions and that are inspiring novel therapeutic strategies for clinical application.
TRPV4 Mechanotransduction in Fibrosis
Authors: Adapala Ravi K. , Katari Venkatesh , Teegala Lakshminarayan Reddy , Thodeti Sathwika , Paruchuri Sailaja , Thodeti Charles K.
Published: November 2021

Fibrosis is an irreversible, debilitating condition marked by the excessive production of extracellular matrix and tissue scarring that eventually results in organ failure and disease. Differentiation of fibroblasts to hypersecretory myofibroblasts is the key event in fibrosis. Although both soluble and mechanical factors are implicated in fibroblast differentiation, much of the focus is on TGF-β signaling, but to date, there are no specific drugs available for the treatment of fibrosis. In this review, we describe the role for TRPV4 mechanotransduction in cardiac and lung fibrosis, and we propose TRPV4 as an alternative therapeutic target for fibrosis.
Vascular Dysfunction in Preeclampsia
Authors: Opichka Megan A. , Rappelt Matthew W. , Gutterman David D. , Grobe Justin L. , McIntosh Jennifer J.
Published: November 2021

Preeclampsia is a life-threatening pregnancy-associated cardiovascular disorder characterized by hypertension and proteinuria at 20 weeks of gestation. Though its exact underlying cause is not precisely defined and likely heterogenous, a plethora of research indicates that in some women with preeclampsia, both maternal and placental vascular dysfunction plays a role in the pathogenesis and can persist into the postpartum period. Potential abnormalities include impaired placentation, incomplete spiral artery remodeling, and endothelial damage, which are further propagated by immune factors, mitochondrial stress, and an imbalance of pro- and antiangiogenic substances. While the field has progressed, current gaps in knowledge include detailed initial molecular mechanisms and effective treatment options. Newfound evidence indicates that vasopressin is an early mediator and biomarker of the disorder, and promising future therapeutic avenues include mitigating mitochondrial dysfunction, excess oxidative stress, and the resulting inflammatory state. In this review, we provide a detailed overview of vascular defects present during preeclampsia and connect well-established notions to newer discoveries at the molecular, cellular, and whole-organism levels.
STAT6 Deficiency Attenuates Myeloid Fibroblast Activation and Macrophage Polarization in Experimental Folic Acid Nephropathy
Authors: Jiao Baihai , An Changlong , Du Hao , Tran Melanie , Wang Penghua , Zhou Dong , Wang Yanlin
Published: November 2021

Renal fibrosis is a pathologic feature of chronic kidney disease, which can lead to end-stage kidney disease. Myeloid fibroblasts play a central role in the pathogenesis of renal fibrosis. However, the molecular mechanisms pertaining to myeloid fibroblast activation remain to be elucidated. In the present study, we examine the role of signal transducer and activator of transcription 6 (STAT6) in myeloid fibroblast activation, macrophage polarization, and renal fibrosis development in a mouse model of folic acid nephropathy. STAT6 is activated in the kidney with folic acid nephropathy. Compared with folic-acid-treated wild-type mice, STAT6 knockout mice had markedly reduced myeloid fibroblasts and myofibroblasts in the kidney with folic acid nephropathy. Furthermore, STAT6 knockout mice exhibited significantly less CD206 and PDGFR-β dual-positive fibroblast accumulation and M2 macrophage polarization in the kidney with folic acid nephropathy. Consistent with these findings, STAT6 knockout mice produced less extracellular matrix protein, exhibited less severe interstitial fibrosis, and preserved kidney function in folic acid nephropathy. Taken together, these results have shown that STAT6 plays a critical role in myeloid fibroblasts activation, M2 macrophage polarization, extracellular matrix protein production, and renal fibrosis development in folic acid nephropathy. Therefore, targeting STAT6 may provide a novel therapeutic strategy for fibrotic kidney disease.
The Motility and Mesenchymal Features of Breast Cancer Cells Correlate with the Levels and Intracellular Localization of Transglutaminase Type 2
Authors: Bianchi Nicoletta , Brugnoli Federica , Grassilli Silvia , Bourgeois Karine , Keillor Jeffrey W. , Bergamini Carlo M. , Aguiari Gianluca , Volinia Stefano , Bertagnolo Valeria
Published: November 2021

We have investigated motility in breast cancer cell lines in association with the expression of Transglutaminase type 2 (TG2) as well as upon the administration of Doxorubicin (Dox), an active cytotoxic agent that is employed in chemotherapy. The exposure of MCF-7 cells to the drug increased TG2 levels, triggering epithelial–mesenchymal transition (EMT), thereby supporting cell motility. The effects of Dox on the movement of MCF-7 cells were counteracted by treatment with NC9, a TG2 inhibitor, which induced morphological changes and also reduced the migration of MDA-MB-231 cells exhibiting high levels of TG2. The physical association of TG2 with the cytoskeletal component vimentin appeared pivotal both in drug-treated MCF-7 and in MDA-MB-231 cells and seemed to be independent of the catalytic activity of TG2. NC9 altered the subcellular distribution of TG2 and, consequently, the co-localization of TG2 with vimentin. Furthermore, NC9 induced a nuclear accumulation of TG2 as a prelude to TG2-dependent gene expression modifications. Since enzyme activity can affect both motility and nuclear functions, targeting of this protein could represent a method to improve therapeutic interventions in breast tumors, particularly those to control progression and to limit drug resistance.
NSC828779 Alleviates Renal Tubulointerstitial Lesions Involving Interleukin-36 Signaling in Mice
Authors: Yang Shin-Ruen , Hung Szu-Chun , Chu Lichieh Julie , Hua Kuo-Feng , Wei Chyou-Wei , Tsai I-Lin , Kao Chih-Chin , Sung Chih-Chien , Chu Pauling , Wu Chung-Yao , Chen Ann , Wu Alexander T. H. , Liu Feng-Cheng , Huang Hsu-Shan , Ka Shuk-Man
Published: November 2021

Renal tubulointerstitial lesions (TILs), a common pathologic hallmark of chronic kidney disease that evolves to end-stage renal disease, is characterized by progressive inflammation and pronounced fibrosis of the kidney. However, current therapeutic approaches to treat these lesions remain largely ineffectual. Previously, we demonstrated that elevated IL-36α levels in human renal tissue and urine are implicated in impaired renal function, and IL-36 signaling enhances activation of NLRP3 inflammasome in a mouse model of TILs. Recently, we synthesized NSC828779, a salicylanilide derivative (protected by U.S. patents with US 8975255 B2 and US 9162993 B2), which inhibits activation of NF-κB signaling with high immunomodulatory potency and low IC50, and we hypothesized that it would be a potential drug candidate for renal TILs. The current study validated the therapeutic effects of NSC828779 on TILs using a mouse model of unilateral ureteral obstruction (UUO) and relevant cell models, including renal tubular epithelial cells under mechanically induced constant pressure. Treatment with NSC828779 improved renal lesions, as demonstrated by dramatically reduced severity of renal inflammation and fibrosis and decreased urinary cytokine levels in UUO mice. This small molecule specifically inhibits the IL-36α/NLRP3 inflammasome pathway. Based on these results, the beneficial outcome represents synergistic suppression of both the IL-36α-activated MAPK/NLRP3 inflammasome and STAT3- and Smad2/3-dependent fibrogenic signaling. NSC828779 appears justified as a new drug candidate to treat renal progressive inflammation and fibrosis.
Structural Insights into Ca2+ Permeation through Orai Channels
Authors: Li Yang , Yang Xue , Shen Yuequan
Published: November 2021

Orai channels belong to the calcium release-activated calcium (CRAC) channel family. Orai channels are responsible for the influx of extracellular Ca2+ that is triggered by Ca2+ depletion from the endoplasmic reticulum (ER); this function is essential for many types of non-excitable cells. Extensive structural and functional studies have advanced the knowledge of the molecular mechanism by which Orai channels are activated. However, the gating mechanism that allows Ca2+ permeation through Orai channels is less well explained. Here, we reviewed and summarized the existing structural studies of Orai channels. We detailed the structural features of Orai channels, described structural comparisons of their closed and open states, and finally proposed a “push–pull” model of Ca2+ permeation.
DNA Methylation in Genetic and Sporadic Forms of Neurodegeneration: Lessons from Alzheimer’s, Related Tauopathies and Genetic Tauopathies
Authors: Zimmer-Bensch Geraldine , Zempel Hans
Published: November 2021

Genetic and sporadic forms of tauopathies, the most prevalent of which is Alzheimer’s Disease, are a scourge of the aging society, and in the case of genetic forms, can also affect children and young adults. All tauopathies share ectopic expression, mislocalization, or aggregation of the microtubule associated protein TAU, encoded by the MAPT gene. As TAU is a neuronal protein widely expressed in the CNS, the overwhelming majority of tauopathies are neurological disorders. They are characterized by cognitive dysfunction often leading to dementia, and are frequently accompanied by movement abnormalities such as parkinsonism. Tauopathies can lead to severe neurological deficits and premature death. For some tauopathies there is a clear genetic cause and/or an epigenetic contribution. However, for several others the disease etiology is unclear, with few tauopathies being environmentally triggered. Here, we review current knowledge of tauopathies listing known genetic and important sporadic forms of these disease. Further, we discuss how DNA methylation as a major epigenetic mechanism emerges to be involved in the disease pathophysiology of Alzheimer’s, and related genetic and non-genetic tauopathies. Finally, we debate the application of epigenetic signatures in peripheral blood samples as diagnostic tools and usages of epigenetic therapy strategies for these diseases.
Deletion of TSPO Causes Dysregulation of Cholesterol Metabolism in Mouse Retina
Authors: Farhan Fahad , Almarhoun Mohammad , Wong Aileen , Findlay Amy S. , Bartholomew Chris , Williams Mark T. S. , Hurd Toby W. , Shu Xinhua
Published: November 2021

Cholesterol dysregulation has been implicated in age-related macular degeneration (AMD), the most common cause of visual impairment in the elderly. The 18 KDa translocator protein (TSPO) is a mitochondrial outer membrane protein responsible for transporting cholesterol from the mitochondrial outer membrane to the inner membrane. TSPO is highly expressed in retinal pigment epithelial (RPE) cells, and TSPO ligands have shown therapeutic potential for the treatment of AMD. Here, we characterized retinal pathology of Tspo knockout (KO) mice using histological, immunohistochemical, biochemical and molecular biological approaches. We found that Tspo KO mice had normal retinal morphology (by light microscopy) but showed elevated levels of cholesterol, triglycerides and phospholipids with perturbed cholesterol efflux in the RPE cells of Tspo KO mice. Expression of cholesterol-associated genes (Nr1h3, Abca1, Abcg1, Cyp27a1 and Cyp46a1) was significantly downregulated, and production of pro-inflammatory cytokines was markedly increased in Tspo KO retinas. Furthermore, microglial activation was also observed in Tspo KO mouse retinas. These findings provide new insights into the function of TSPO in the retina and may aid in the design of new therapeutic strategies for the treatment of AMD.
Endothelial Dysfunction through Oxidatively Generated Epigenetic Mark in Respiratory Viral Infections
Authors: Vlahopoulos Spiros , Wang Ke , Xue Yaoyao , Zheng Xu , Boldogh Istvan , Pan Lang
Published: November 2021

The bronchial vascular endothelial network plays important roles in pulmonary pathology during respiratory viral infections, including respiratory syncytial virus (RSV), influenza A(H1N1) and importantly SARS-Cov-2. All of these infections can be severe and even lethal in patients with underlying risk factors.A major obstacle in disease prevention is the lack of appropriate efficacious vaccine(s) due to continuous changes in the encoding capacity of the viral genome, exuberant responsiveness of the host immune system and lack of effective antiviral drugs. Current management of these severe respiratory viral infections is limited to supportive clinical care. The primary cause of morbidity and mortality is respiratory failure, partially due to endothelial pulmonary complications, including edema. The latter is induced by the loss of alveolar epithelium integrity and by pathological changes in the endothelial vascular network that regulates blood flow, blood fluidity, exchange of fluids, electrolytes, various macromolecules and responses to signals triggered by oxygenation, and controls trafficking of leukocyte immune cells. This overview outlines the latest understanding of the implications of pulmonary vascular endothelium involvement in respiratory distress syndrome secondary to viral infections. In addition, the roles of infection-induced cytokines, growth factors, and epigenetic reprogramming in endothelial permeability, as well as emerging treatment options to decrease disease burden, are discussed.
Heterogeneity of Cancer Stem Cells in Tumorigenesis, Metastasis, and Resistance to Antineoplastic Treatment of Head and Neck Tumours
Authors: Cirillo Nicola , Wu Carmen , Prime Stephen S.
Published: November 2021

The discovery of a small subset of cancer cells with self-renewal properties that can give rise to phenotypically diverse tumour populations has shifted our understanding of cancer biology. Targeting cancer stem cells (CSCs) is becoming a promising therapeutic strategy in various malignancies, including head and neck squamous cell carcinoma (HNSCC). Diverse sub-populations of head and neck cancer stem cells (HNCSCs) have been identified previously using CSC specific markers, the most common being CD44, Aldehyde Dehydrogenase 1 (ALDH1), and CD133, or by side population assays. Interestingly, distinct HNCSC subsets play different roles in the generation and progression of tumours. This article aims to review the evidence for a role of specific CSCs in HNSCC tumorigenesis, invasion, and metastasis, together with resistance to treatment.
Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response
Authors: Biagi Carlos Alberto Oliveira , Cury Sarah Santiloni , Alves Cleidson Pádua , Rabhi Nabil , Silva Wilson Araujo , Farmer Stephen R. , Carvalho Robson Francisco , Batista Miguel Luiz
Published: November 2021

Adipose tissue has been classified based on its morphology and function as white, brown, or beige/brite. It plays an essential role as a regulator of systemic metabolism through paracrine and endocrine signals. Recently, multiple adipocyte subtypes have been revealed using RNA sequencing technology, going beyond simply defined morphology but also by their cellular origin, adaptation to metabolic stress, and plasticity. Here, we performed an in-depth analysis of publicly available single-nuclei RNAseq from adipose tissue and utilized a workflow template to characterize adipocyte plasticity, heterogeneity, and secretome profiles. The reanalyzed dataset led to the identification of different subtypes of adipocytes including three subpopulations of thermogenic adipocytes, and provided a characterization of distinct transcriptional profiles along the adipocyte trajectory under thermogenic challenges. This study provides a useful resource for further investigations regarding mechanisms related to adipocyte plasticity and trans-differentiation.
Implantation of Covered Stent for Coarctation of the Aorta and Secondary Hypertension in Adolescents—Case Report
Authors: Ghiragosian-Rusu Simina-Elena , Blesneac Cristina , Sglimbea Anca , Ghiragosian Claudiu , Hadadi Laszlo , Făgărășan Amalia , Togănel Rodica
Published: November 2021

Introduction: Coarctation of the aorta represents a narrowing of the thoracic aorta. Hypertensive patients with blood pressure differences ≥20 millimetres of mercury have an indication for surgical or interventional treatment. Implantation of a covered stent became the preferred therapy for the management of this pathology in adolescents/adults. Case report: We report the case of a 14-year-old male sportsman, who presented in the emergency room with headache, dizziness, and tinnitus. The clinical exam revealed blood pressure differences between the upper and lower limbs of up to 50 mmHg. Based on the clinical and paraclinical data, we established the diagnosis of coarctation of the aorta and severe secondary arterial hypertension. The case was discussed by a multidisciplinary team and accepted for covered stent implantation. The 24 h blood pressure Holter monitoring after the procedure indicated the persistence of stage I arterial hypertension. Conclusions: Coarctation of the aorta is a congenital cardiovascular anomaly with high morbidity and mortality rates. Arterial hypertension, heart failure, and aortic dissection are complications of this pathology, some of them being sometimes direct consequences of secondary hypertension. Periodic cardiology follow up after the procedure is mandatory to assess the hemodynamic response, to identify potential complications, and to stratify the cardiovascular risk.
Improving the Quality of Life of Cancer Survivors in School: Consensus Recommendations Using a Delphi Study
Authors: Galán Santiago , Tomé-Pires Catarina , Roy Rubén , Castarlenas Elena , Racine Mélanie , Jensen Mark P. , Miró Jordi
Published: November 2021

Successful school re-entry is important for children following cancer treatment. However, this process is a challenge for teachers. Objectives: To identify (1) the difficulties and needs that teachers have in helping youth cancer survivors be successful in school, (2) the most effective resources that teachers are currently using for helping them, and (3) the ideal contents for a program that could help teachers in this area. Methods: Twenty-eight teachers participated in a Delphi study. Results: A lack of knowledge regarding how to best help and having to deal with the student’s problems were identified as difficulties. Specific training, psychological support, and advice from health professionals were the most commonly reported needs. Maintaining contact with the family and the students and providing personalized attention were viewed as the most useful resources. Finally, knowledge about the disease itself and how to facilitate successful school re-entry were identified as important program components. Conclusion: The findings provide important new information regarding the lack of both resources and support for teachers who seek to help youth cancer survivors. The findings can be used to inform the development of an intervention to help teachers become more successful in facilitating successful school re-entry.
Associations between Physical Activity and Mental Health in Iranian Adolescents during the COVID-19 Pandemic: An Accelerometer-Based Study
Authors: Ghorbani Saeed , Afshari Mostafa , Eckelt Melanie , Dana Amir , Bund Andreas
Published: November 2021

Using self-reported questionnaires, several studies found that social isolation during the COVID-19 pandemic has significantly changed the level of physical activity (PA) in children and adolescents. Since the objectivity of self-reported PA is limited in several ways, we used modern accelerometers in this study to assess the PA levels of male and female adolescents during the pandemic-related lockdown. Moreover, the association of PA with mental health of the adolescents were analyzed. A total of 136 students (76 girls, mean age of 16.28 ± 0.97 years) from various schools in Iran wore the accelerometer (ActiGraph GT3X-BT) for seven consecutive days. Mental health was measured through the Depression, Anxiety, Stress Scale-21 of Lovibond and Lovibond (1995). Descriptive statistics and independent t-tests were used to analyze the PA levels of male and female students, and linear regressions were computed to examine whether PA predicts mental health status. On average, the daily time spent in moderate-to-vigorous PA (MVPA) was 18.47 min, which is clearly below the WHO guideline of at least 60 min of MVPA per day. Only 4% of adolescents showed MVPA for more than 30 min per day. Male students were significantly more active than their female peers (p = 0.015). As expected, MVPA was negatively associated with mental health issues such as depression, anxiety, and stress. However, the majority of adolescents reported symptoms of mild-to-moderate mental health disorders. These results emphasize the need for targeted strategies and offerings geared to young people’s needs and preferences to promote an adequate level of PA and good mental health during and after the ongoing pandemic.
Associations between Child and Family Level Correlates and Behavioural Patterns in School-Aged Children
Authors: D’Souza Ninoshka J. , Zheng Miaobing , Abbott Gavin , Lioret Sandrine , Hesketh Kylie D.
Published: November 2021

Identifying correlates of behavioural patterns are important to target population sub-groups at increased health risk. The aim was to investigate correlates of behavioural patterns comprising four behavioural domains in children. Data were from the HAPPY study when children were 6–8 years (n = 335) and 9–11 years (n = 339). Parents reported correlate and behavioural data (dietary intake, physical activity, sedentary behaviour, and sleep). Behavioural data were additionally captured using accelerometers. Latent profile analysis was used to derive patterns. Patterns were identified as healthy, unhealthy, and mixed at both time points. Multinomial logistic regression tested for associations. Girls were more likely to display healthy patterns at 6–8 years and display unhealthy and mixed patterns at 9–11 years than boys, compared to other patterns at the corresponding ages. Increased risk of displaying the unhealthy pattern with higher age was observed at both timepoints. At 9–11 years, higher parental working hours were associated with lower risk of displaying mixed patterns compared to the healthy pattern. Associations observed revealed girls and older children to be at risk for unhealthy patterns, warranting customisation of health efforts to these groups. The number of behaviours included when deriving patterns and the individual behaviours that dominate each pattern appear to be drivers of the associations for child level, but not for family level, correlates.
The Role of Occupational Therapy in Managing Food Selectivity of Children with Autism Spectrum Disorder: A Scoping Review
Authors: Reche-Olmedo Laura , Torres-Collado Laura , Compañ-Gabucio Laura María , Garcia-de-la-Hera Manuela
Published: November 2021

Food selectivity is common in children with autism spectrum disorder (ASD). It can be defined as the unwillingness to eat common or new foods, resulting in a lack of variety in the diet or limited food consumption for multiple reasons, such as inflexibility or sensory alterations. We conducted a peer scoping review to describe the interventions that are carried out from occupational therapy (OT) in children with ASD with food selectivity. Two authors independently searched the databases PubMed, Scopus, Web of Science, and EMBASE, as well as the OT journals indexed in Journal Citation Reports. Articles exploring OT interventions in children (≤12 years) with ASD and food selectivity, published in Spanish or English, with experimental design, and with full text available were included. Of the 1445 articles identified, 8 articles met the inclusion criteria. Three main intervention categories were identified: sensory–behavioral, family focused, and other interventions. Most of the interventions from OT were aimed at treating sensory–behavioral aspects. Only three articles described interventions led exclusively by occupational therapists, and the rest were led by a multidisciplinary team. Finally, although these interventions are not exclusive to OT, occupational therapists can participate together with other professionals as an essential component in the treatment of food selectivity in children with ASD.
Vertigo and Dizziness in Children: An Update
Authors: Fancello Virginia , Palma Silvia , Monzani Daniele , Pelucchi Stefano , Genovese Elisabetta , Ciorba Andrea
Published: November 2021

Background: Vertigo and dizziness are relatively infrequent in paediatric patients, but specific data on the prevalence of these disorders are limited and influenced by various factors, including the age of the examined population. These conditions often have a significant impact on patients’ and parents’ quality of life. The aim of this paper is to investigate the prevalence of different aetiologies of vertigo in the paediatric population through a systematic review. Methods: According to PRISMA guidelines, a systematic review of the literature was performed. Medline and Embase were searched from January 2011 through to 10 September 2021. The search yielded 1094 manuscripts, which were reduced to 7 upon the application of inclusion criteria. Results: A total of 2470 paediatric patients were evaluated by the selected papers. Vestibular Migraine was the most frequently diagnosed condition, occurring alone or in association with other diseases. Overall, audio-vestibular disorders represented the second cause of vertigo, and the prevalence appears to increase according to age growth. Over the years, even though we assisted in the amelioration of diagnostic rates, partially related to an improvement in diagnostic tools, the aetiology of vertigo remains still unclear in a variable percentage of patients. Conclusion: Vertigo in children, despite being an uncommon symptom, requires a multidisciplinary approach, often involving Paediatricians, Neurologists and Otorhinolaryngologists. A comprehensive evaluation of children suffering from vertigo is crucial for establishing a successful therapy and reducing parental worries.
Strategy to Develop and Evaluate a Multiplex RT-ddPCR in Response to SARS-CoV-2 Genomic Evolution
Authors: Van Poelvoorde Laura A. E. , Gand Mathieu , Fraiture Marie-Alice , De Keersmaecker Sigrid C. J. , Verhaegen Bavo , Van Hoorde Koenraad , Cay Ann Brigitte , Balmelle Nadège , Herman Philippe , Roosens Nancy
Published: November 2021

The worldwide emergence and spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) since 2019 has highlighted the importance of rapid and reliable diagnostic testing to prevent and control the viral transmission. However, inaccurate results may occur due to false negatives (FN) caused by polymorphisms or point mutations related to the virus evolution and compromise the accuracy of the diagnostic tests. Therefore, PCR-based SARS-CoV-2 diagnostics should be evaluated and evolve together with the rapidly increasing number of new variants appearing around the world. However, even by using a large collection of samples, laboratories are not able to test a representative collection of samples that deals with the same level of diversity that is continuously evolving worldwide. In the present study, we proposed a methodology based on an in silico and in vitro analysis. First, we used all information offered by available whole-genome sequencing data for SARS-CoV-2 for the selection of the two PCR assays targeting two different regions in the genome, and to monitor the possible impact of virus evolution on the specificity of the primers and probes of the PCR assays during and after the development of the assays. Besides this first essential in silico evaluation, a minimal set of testing was proposed to generate experimental evidence on the method performance, such as specificity, sensitivity and applicability. Therefore, a duplex reverse-transcription droplet digital PCR (RT-ddPCR) method was evaluated in silico by using 154 489 whole-genome sequences of SARS-CoV-2 strains that were representative for the circulating strains around the world. The RT-ddPCR platform was selected as it presented several advantages to detect and quantify SARS-CoV-2 RNA in clinical samples and wastewater. Next, the assays were successfully experimentally evaluated for their sensitivity and specificity. A preliminary evaluation of the applicability of the developed method was performed using both clinical and wastewater samples.
Evaluation of Climate Change on Streamflow, Sediment, and Nutrient Load at Watershed Scale
Authors: Parajuli Prem B. , Risal Avay
Published: November 2021

This study evaluated changes in climatic variable impacts on hydrology and water quality in Big Sunflower River Watershed (BSRW), Mississippi. Site-specific future time-series precipitation, temperature, and solar radiation data were generated using a stochastic weather generator LARS-WG model. For the generation of climate scenarios, Representative Concentration Pathways (RCPs), 4.5 and 8.5 of Global Circulation Models (GCMs): Hadley Center Global Environmental Model (HadGEM) and EC-EARTH, for three (2021–2040, 2041–2060 and 2061–2080) future climate periods. Analysis of future climate data based on six ground weather stations located within BSRW showed that the minimum temperature ranged from 11.9 °C to 15.9 °C and the maximum temperature ranged from 23.2 °C to 28.3 °C. Similarly, the average daily rainfall ranged from 3.6 mm to 4.3 mm. Analysis of changes in monthly average maximum/minimum temperature showed that January had the maximum increment and July/August had a minimum increment in monthly average temperature. Similarly, maximum increase in monthly average rainfall was observed during May and maximum decrease was observed during September. The average monthly streamflow, sediment, TN, and TP loads under different climate scenarios varied significantly. The change in average TN and TP loads due to climate change were observed to be very high compared to the change in streamflow and sediment load. The monthly average nutrient load under two different RCP scenarios varied greatly from as low as 63% to as high as 184%, compared to the current monthly nutrient load. The change in hydrology and water quality was mainly attributed to changes in surface temperature, precipitation, and stream flow. This study can be useful in the development and implementation of climate change smart management of agricultural watersheds.
Chitosan-Based Antimicrobial Coating for Improving Postharvest Shelf Life of Pineapple
Authors: Basumatary Indra Bhusan , Mukherjee Avik , Katiyar Vimal , Kumar Santosh , Dutta Joydeep
Published: November 2021

Rapid postharvest losses and quality deteriorations in pineapple are major challenges to growers and handlers. Chitosan-based coatings on fruit surfaces have gained importance in recent years to enhance postharvest shelf life of the fruits. In this study, aloe vera gel was added as a natural antioxidant in chitosan-based composite coating containing ZnO nanoparticles. The developed formulation was applied on the surface of freshly harvested pineapple fruits. ZnO nanoparticles were used as an antimicrobial agent. Coated pineapple fruits were evaluated for weight loss, total soluble solids, titratable acidity, decay index, maturity index, and sensory attributes, including visual appearance, periodically at 5 day interval during storage. The results showed that the coating of the fruit reduced weight loss by about 5%, and also delayed ripening and oxidative decay compared to the uncoated fruit. Thus, the developed coating formulation is a promising sustainable solution to reduce postharvest losses and to extend shelf life of pineapples.
Electrodeposition, Characterization, and Corrosion Behavior of CoCrFeMnNi High-Entropy Alloy Thin Films
Authors: Popescu Ana-Maria Julieta , Branzoi Florina , Constantin Ionut , Anastasescu Mihai , Burada Marian , Mitrică Dumitru , Anasiei Ioana , Olaru Mihai-Tudor , Constantin Virgil
Published: November 2021

Potentiostatic electrodeposition was used to obtain CoCrFeMnNi high-entropy alloy (HEA) thin films on copper substrate. An electrolyte based on a DMSO (dimethyl sulfoxide)-CH3CN (acetonitrile) organic compound was used for the HEA deposition. The microstructure of the high-entropy deposits before and after corrosion in artificial seawater was investigated by scanning electron microscopy (SEM) and energy dispersive spectrometry (EDS) investigation. SEM analysis revealed that compact and uniform film consists of compact and uniform 50 nm–5 μm particles that form the HEA films. The successful co-deposition of all five elements was highlighted by the energy dispersive spectrometry investigation (EDS). Electrochemical measurements carried out in an aerated artificial seawater solution under ambient conditions demonstrated the promising potential for application in the field of anti-corrosion protection, due to the protective behavior of the HEA thin films.
In-Depth Analysis of Ransom Note Files
Authors: Lemmou Yassine , Lanet Jean-Louis , Souidi El Mamoun
Published: November 2021

During recent years, many papers have been published on ransomware, but to the best of our knowledge, no previous academic studies have been conducted on ransom note files. In this paper, we present the results of a depth study on filenames and the content of ransom files. We propose a prototype to identify the ransom files. Then we explore how the filenames and the content of these files can minimize the risk of ransomware encryption of some specified ransomware or increase the effectiveness of some ransomware detection tools. To achieve these objectives, two approaches are discussed in this paper. The first uses Latent Semantic Analysis (LSA) to check similarities between the contents of files. The second uses some Machine Learning models to classify the filenames into two classes—ransom filenames and benign filenames.
Strategy for the Development of a New Lipstick Formula
Authors: Rigano Luigi , Montoli Marta
Published: November 2021

The strategy to adopt for the development of a new lipstick formula requires, as a first step, the definition of the most important characteristics of a modern product. Successively, the identification of the key properties of any innovative ingredient is necessary. Then, a comprehension of the key parameters in the establishment of a stable equilibrium among the different formula components in the solid state is important. Moreover, it is necessary to study the interactions among the new ingredients and the other structural components in the formula. Finally, an evaluation of the sensory properties of the different final formulae for fine-tuning of practical performances needs to be carefully carried out. In this study, a systematic formulation approach tried to obtain a new lipstick formula using a new vegetal-derived emollient *(INCLUDING COCO-CAPRYLATE/CAPRATE, HYDROGENATED OLIVE OIL UNSAPONIFIABLES) with sensorial properties similar to some types of silicones. Some application trials of the new raw material were carried out. The following aspects of this ingredient were investigated: (1) Compatibility and thickening with waxes, (2) dispersion power of pigments, and (3) influence on sensory characteristics of the formulated lipstick. This new emollient has been shown to improve some aspects of a lipstick formula, in particular shine, homogeneity, and covering effect. The optimization of the formula, in order to increase the sensation of softness on the lips, is described. *Plantasens Olive LD SP ECO, supplier Clariant Gmbh.
Rolling Texture of Cu–30%Zn Alloy Using Taylor Model Based on Twinning and Coplanar Slip
Authors: Hsiao Shih-Chieh , Lin Sin-Ying , Chen Huang-Jun , Hsieh Ping-Yin , Kuo Jui-Chao
Published: November 2021

A modified Taylor model, hereafter referred to as the MTCS (Mechanical-Twinning-with-Coplanar-Slip)-model, is proposed in the present work to predict weak texture components in the shear bands of brass-type fcc metals with a twin–matrix lamellar (TML) structure. The MTCS-model considers two boundary conditions (i.e., twinning does not occur in previously twinned areas and coplanar slip occurs in the TML region) to simulate the rolling texture of Cu–30%Zn. In the first approximation, texture simulation using the MTCS-model revealed brass-type textures, including Y{1 1 1} <1 1 2> and Z{1 1 1} <1 1 0> components, which correspond to the observed experimental textures. Single orientations of C(1 1 2)[1¯ 1¯ 1] and S’(1 2 3)[4¯ 1¯ 2] were applied to the MTCS-model to understand the evolution of Y and Z components. For the Y orientation, the C orientation rotates toward T(5 5 2)[1 1 5] by twinning after 30% reduction and then toward Y(1 1 1)[1 1 2] by coplanar slip after over 30% reduction. For the Z orientation, the S’ orientation rotates toward T’(3 2 1)[2 1¯ 4¯] by twinning after 30% reduction and then toward Z(1 1 1)[1 0 1¯] by coplanar slip after over 30% reduction.