bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigating the Effects of Sex Hormones on Macrophage Polarization
Authors: Enright Sophie , Werstuck Geoff H.
Published: January 2024

Sex differences in the development and progression of cardiovascular disease are well established, but the effects of sex hormones on macrophage polarization and pro-atherogenic functions are not well described. We hypothesize that sex hormones directly modulate macrophage polarization, and thereby regulate the progression of atherosclerosis. Bone marrow-derived monocytes from adult male and female C57BL/6 mice were differentiated into macrophages using macrophage colony-stimulating factor (20 ng/mL) and pre-treated with either 17β-estradiol (100 nM), testosterone (100 nM), or a vehicle control for 24 h. Macrophages were polarized into pro- or anti-inflammatory phenotypes and the effects of sex hormone supplementation on the gene expression of macrophage phenotypic markers were assessed using RT-qPCR. Inflammatory markers, including IL-1β, were quantified using an addressable laser bead immunoassay. A transwell migration assay was used to determine changes in macrophage migration. Sex differences were observed in macrophage polarization, inflammatory responses, and migration. Pre-treatment with 17β-estradiol significantly impaired the gene expression of inflammatory markers and the production of IL-1β in inflammatory macrophages. In anti-inflammatory macrophages, 17β-estradiol significantly upregulated the expression of anti-inflammatory markers and enhanced migration. Pre-treatment with testosterone enhanced anti-inflammatory mRNA expression and impaired the production of IL-1β. Our observations suggest a protective role of 17β-estradiol in atherogenesis that may contribute to the sexual dimorphisms in cardiovascular disease observed in human patients.
Clofarabine Preconditioning followed by Allogeneic Transplant Using TBI and Post-Transplant Cyclophosphamide for Relapsed Refractory Leukemia
Authors: Naik Seema , Rakszawski Kevin , Zheng Hong , Claxton David , Minagawa Kentaro , Mineishi Shin
Published: January 2024

Acute myeloid leukemia patients with induction failure or relapsed refractory disease have minimal chance of achieving remission with subsequent treatments. Several trials have shown the feasibility of clofarabine-based conditioning in allogeneic stem cell transplants (allo-HSCT) for non-remission AML patients. Pre-transplant conditioning with clofarabine followed by reduced-intensity allo-HSCT has also demonstrated a potential benefit in those patients with human leukocyte antigen (HLA)-identical donors, but it is not commonly used in haploidentical and mismatched transplants. In this case report, we describe our experience of seven cases of non-remission AML who received clofarabine preconditioning followed by an allo-HSCT with PTCy. The 2-year overall survival and disease-free survival was 83.3% (95% confidence interval (CI): 27.3–97.9%) and 85.7% (95% CI: 33.4–97.9%). Median days of neutrophil and platelet recovery were 16 (range of 13–23) and 28 (range of 17–75), respectively. The cumulative incidence of grade II-IV acute graft-versus-host disease (GVHD) at day 100 and chronic GVHD at 1-year showed 28.6% (95% CI: 8–74.2%) and 28.6% (95% CI: 3–63.9%), respectively. The two-year relapse rate was 14.3% (95% CI: 2.14–66.6%). One-year GVHD-free relapse-free survival (GFRS) at 1-year was 71.4% (95% CI: 25.8–92%). Our patients showed successful outcomes with clofarabine preconditioning to reduce the leukemic burden at the pre-transplant period followed by PTCy to reduce GVHD resulting in lower relapsed rate and better GFRS in these patients.
Body Composition in Chronic Liver Disease
Authors: Nishikawa Hiroki , Kim Soo Ki , Asai Akira
Published: January 2024

Body composition has recently been attracting people’s attention, not only from a cosmetic standpoint but also from the perspective of health and longevity. The body is classified into three components: fat, bone, and lean soft tissue, and it is common to see an increase in body fat and a decrease in total body muscle mass with aging. Aging-related loss of muscle mass and muscle function is referred to as primary sarcopenia, while sarcopenia caused by disease-specific conditions is referred to as secondary sarcopenia. On the other hand, the liver-muscle axis has been attracting attention in recent years, and it has become clear that the liver and the skeletal muscles interact with each other. In particular, patients with cirrhosis are prone to secondary sarcopenia due to protein-energy malnutrition, which is a characteristic pathophysiology of the disease, suggesting the importance of the organ–organ network. In this review, we would like to outline the latest findings in this field, with a focus on body composition in liver diseases such as liver cirrhosis, fatty liver disease, alcoholic liver disease, and hepatocellular carcinoma.
Perinatal Tissue-Derived Stem Cells: An Emerging Therapeutic Strategy for Challenging Neurodegenerative Diseases
Authors: Bruno Annalisa , Milillo Cristina , Anaclerio Federico , Buccolini Carlotta , Dell’Elice Anastasia , Angilletta Ilaria , Gatta Marco , Ballerini Patrizia , Antonucci Ivana
Published: January 2024

Over the past 20 years, stem cell therapy has been considered a promising option for treating numerous disorders, in particular, neurodegenerative disorders. Stem cells exert neuroprotective and neurodegenerative benefits through different mechanisms, such as the secretion of neurotrophic factors, cell replacement, the activation of endogenous stem cells, and decreased neuroinflammation. Several sources of stem cells have been proposed for transplantation and the restoration of damaged tissue. Over recent decades, intensive research has focused on gestational stem cells considered a novel resource for cell transplantation therapy. The present review provides an update on the recent preclinical/clinical applications of gestational stem cells for the treatment of protein-misfolding diseases including Alzheimer’s disease (AD), Parkinson’s disease (PD), Huntington’s disease (HD) and amyotrophic lateral sclerosis (ALS). However, further studies should be encouraged to translate this promising therapeutic approach into the clinical setting.
Caveolin-3 and Caveolin-1 Interaction Decreases Channel Dysfunction Due to Caveolin-3 Mutations
Authors: Benzoni Patrizia , Gazzerro Elisabetta , Fiorillo Chiara , Baratto Serena , Bartolucci Chiara , Severi Stefano , Milanesi Raffaella , Lippi Melania , Langione Marianna , Murano Carmen , Meoni Clarissa , Popolizio Vera , Cospito Alessandro , Baruscotti Mirko , Bucchi Annalisa , Barbuti Andrea
Published: January 2024

Caveolae constitute membrane microdomains where receptors and ion channels functionally interact. Caveolin-3 (cav-3) is the key structural component of muscular caveolae. Mutations in CAV3 lead to caveolinopathies, which result in both muscular dystrophies and cardiac diseases. In cardiomyocytes, cav-1 participates with cav-3 to form caveolae; skeletal myotubes and adult skeletal fibers do not express cav-1. In the heart, the absence of cardiac alterations in the majority of cases may depend on a conserved organization of caveolae thanks to the expression of cav-1. We decided to focus on three specific cav-3 mutations (Δ62-64YTT; T78K and W101C) found in heterozygosis in patients suffering from skeletal muscle disorders. We overexpressed both the WT and mutated cav-3 together with ion channels interacting with and modulated by cav-3. Patch-clamp analysis conducted in caveolin-free cells (MEF-KO), revealed that the T78K mutant is dominant negative, causing its intracellular retention together with cav-3 WT, and inducing a significant reduction in current densities of all three ion channels tested. The other cav-3 mutations did not cause significant alterations. Mathematical modelling of the effects of cav-3 T78K would impair repolarization to levels incompatible with life. For this reason, we decided to compare the effects of this mutation in other cell lines that endogenously express cav-1 (MEF-STO and CHO cells) and to modulate cav-1 expression with an shRNA approach. In these systems, the membrane localization of cav-3 T78K was rescued in the presence of cav-1, and the current densities of hHCN4, hKv1.5 and hKir2.1 were also rescued. These results constitute the first evidence of a compensatory role of cav-1 in the heart, justifying the reduced susceptibility of this organ to caveolinopathies.
The Use of an Antioxidant Enables Accurate Evaluation of the Interaction of Curcumin on Organic Anion-Transporting Polypeptides 4C1 by Preventing Auto-Oxidation
Authors: Sato Toshihiro , Yagi Ayaka , Yamauchi Minami , Kumondai Masaki , Sato Yu , Kikuchi Masafumi , Maekawa Masamitsu , Yamaguchi Hiroaki , Abe Takaaki , Mano Nariyasu
Published: January 2024

Flavonoids have garnered attention because of their beneficial bioactivities. However, some flavonoids reportedly interact with drugs via transporters and may induce adverse drug reactions. This study investigated the effects of food ingredients on organic anion-transporting polypeptide (OATP) 4C1, which handles uremic toxins and some drugs, to understand the safety profile of food ingredients in renal drug excretion. Twenty-eight food ingredients, including flavonoids, were screened. We used ascorbic acid (AA) to prevent curcumin oxidative degradation in our method. Twelve compounds, including apigenin, daidzein, fisetin, genistein, isorhamnetin, kaempferol, luteolin, morin, quercetin, curcumin, resveratrol, and ellagic acid, altered OATP4C1-mediated transport. Kaempferol and curcumin strongly inhibited OATP4C1, and the Ki values of kaempferol (AA(−)), curcumin (AA(−)), and curcumin (AA(+)) were 25.1, 52.2, and 23.5 µM, respectively. The kinetic analysis revealed that these compounds affected OATP4C1 transport in a competitive manner. Antioxidant supplementation was determined to benefit transporter interaction studies investigating the effects of curcumin because the concentration-dependent curve evidently shifted in the presence of AA. In this study, we elucidated the food–drug interaction via OATP4C1 and indicated the utility of antioxidant usage. Our findings will provide essential information regarding food–drug interactions for both clinical practice and the commercial development of supplements.
Influence of Season, Storage Temperature and Time of Sample Collection in Pancreatitis-Associated Protein-Based Algorithms for Newborn Screening for Cystic Fibrosis
Authors: Maier Pia , Jeyaweerasinkam Sumathy , Eberhard Janina , Soueidan Lina , Hämmerling Susanne , Kohlmüller Dirk , Feyh Patrik , Gramer Gwendolyn , Garbade Sven F. , Hoffmann Georg F. , Okun Jürgen G. , Sommerburg Olaf
Published: January 2024

Newborn screening (NBS) for cystic fibrosis (CF) based on pancreatitis-associated protein (PAP) has been performed for several years. While some influencing factors are known, there is currently a lack of information on the influence of seasonal temperature on PAP determination or on the course of PAP blood concentration in infants during the first year of life. Using data from two PAP studies at the Heidelberg NBS centre and storage experiments, we compared PAP determinations in summer and winter and determined the direct influence of temperature. In addition, PAP concentrations measured in CF-NBS, between days 21–35 and 36–365, were compared. Over a 7-year period, we found no significant differences between PAP concentrations determined in summer or winter. We also found no differences in PAP determination after 8 days of storage at 4 °C, room temperature or 37 °C. When stored for up to 3 months, PAP samples remained stable at 4 °C, but not at room temperature (p = 0.007). After birth, PAP in neonatal blood showed a significant increasing trend up to the 96th hour of life (p < 0.0001). During the first year of life, blood PAP concentrations continued to increase in both CF- (36–72 h vs. 36–365 d p < 0.0001) and non-CF infants (36–72 h vs. 36–365 d p < 0.0001). Seasonal effects in central Europe appear to have a limited impact on PAP determination. The impact of the increase in blood PAP during the critical period for CF-NBS and beyond on the applicability and performance of PAP-based CF-NBS algorithms needs to be re-discussed.
Survey of Main Group Metals and Metalloids in Cancer Treatment
Authors: Kostova Irena
Published: January 2024

Cancer is one of the leading causes of human death among all major diseases. Metal-based complexes are considered as the most promising vital part in the existing arsenal of cytotoxic candidates used in cancer therapy and diagnostics. The efforts of many scientific groups resulted in the development of numerous metal-based compounds featuring different biologically active organic ligands in order to modulate their bioactivity. Along with the main representatives as potential therapeutic agents, such as the complexes Pt(II)/Pt(IV), Pd(II), Ru(II)/Ru(III), Ag(I), Au(I)/Au(III), Ti(IV), V(IV) and Ga(III), many other transition metal and lanthanide complexes possessing antiproliferative activity are widely discussed in the literature. However, such drugs remain outside the scope of this review. The main purpose of the current study is to review the potential activity of main group metal- and metalloid-based complexes against the most common cancer cell types, such as carcinomas (lung, liver, breast, kidney, gastric, colorectal, bladder, ovarian, cervical, prostate, etc.); sarcomas; blastomas; lymphomas; multiple myeloma; and melanoma. Overcoming the long disregard of organometallic compounds of metals and metalloids from the main groups, a growing number of emerging anticancer agents remarkably prove this field offers an extensive variety of new options for the design of innovative unexplored chemopharmaceutics. Moreover, some of the metal complexes and organometallic compounds from these elements can exhibit entirely different, specific modes of action and biological targets. Obviously, exploitation of their distinct properties deserves more attention.
Mass-Rearing Conditions Do Not Always Reduce Genetic Diversity: The Case of the Mexican Fruit Fly, Anastrepha ludens (Diptera: Tephritidae)
Authors: Ruiz-Montoya Lorena , Sánchez-Rosario Mayren , López-Gómez Emiliano , Garcia-Bautista Maricela , Canedo-Texón Anahí , Haymer David , Liedo Pablo
Published: January 2024

The application of the sterile insect technique (SIT) requires the adaptation of insects to mass-rearing conditions. It is generally accepted that this adaptation may include a reduction in genetic diversity and an associated loss of desirable characteristics for the effective performance of sterile insects in the field. Here, we compare the genetic diversity of two mass-reared strains of the Mexican fruit fly, Anastrepha ludens, and a wild (WIL) population collected near Tapachula, Mexico, using seven DNA microsatellites as molecular genetic markers. The mass-reared strains were a bisexual laboratory strain (LAB) with approximately 130 generations under mass-rearing and a genetic sexing strain, Tapachula-7 (TA7), also under mass-rearing for 100 generations. Our results revealed an overall low level of genetic differentiation (approximately 15%) among the three strains, with the LAB and WIL populations being genetically most similar and TA7 most genetically differentiated. Although there were some differences in allele frequencies between strains, our results show that overall, the adaptation to mass-rearing conditions did not reduce genetic variability compared to the wild sample in terms of heterozygosity or allelic richness, nor did it appear to alter the level of inbreeding with respect to the wild populations. These results are contrary to the general idea that mass-rearing always results in a reduction in genetic diversity. Overall, our findings can contribute to a better understanding of the impact that adaptation to mass-rearing conditions may have on the genetic make-up of strains.
In Search of Risk Factors: The Origin and Early Stages of Cardiovascular Epidemiology
Authors: Menotti Alessandro , Puddu Paolo Emilio
Published: January 2024

Based mainly on their personal experience, the authors try to describe the origin of cardiovascular disease (CVD) epidemiology and the problems and difficulties practitioners attempted to tackle and solve during the first few decades of this discipline, which started around the middle of the last century. Beyond identifying the characteristics of those who became CVD epidemiologists, a description is given of the initial structures of the involved studies, participation rates, risk factors measurements and standardization, clinical measurements and diagnostic criteria, mortality data collection and coding, data loading and analysis, plus a number of problems still unsolved at the beginning of the 2000s. Despite many obstacles, and the initial hostility of the medical–scientific establishment, CVD epidemiology represented a revolution in researching in the bio-medical field. In the end, it also affected clinical research introducing the use of the quantitative approach bound to mathematical–statistical procedures. After decades of hard work and the development of a number of innovative tools, CVD epidemiology received its deserved recognition, eventually being accepted as a reputable and independent scientific discipline. Yet, in several countries, especially those from Southern Europe, an academic recognition of CVD epidemiology is still lacking.
Encephalitis in the Course of HHV-7 Infection in an Infant
Authors: Moppert Justyna , Łężyk-Ciemniak Eliza , Pawłowska Małgorzata
Published: January 2024

Most cases of acute infections caused by human herpesvirus 7 (HHV-7) are asymptomatic or very mild. Clinical symptoms disappear spontaneously; however, the infection becomes latent and persists for life with periodic asymptomatic reactivation. Little is known about the virus’s ability to cross the blood–brain barrier. Our case of an immunocompetent infant indicates that HHV-7 infection should be considered a cause of neuroinfection, not only in immunocompromised patients but also in the youngest immunocompetent patients.
The Association between Self-Care Activities and Depression in Adult Patients with Type 2 Diabetes in Saudi Arabia: A Cross-Sectional Web-Based Survey Study
Authors: Kurdi Sawsan M. , Alamer Ahmad , Albaggal Aya , Alsuwaiket Marwa , Alotaibi Fawaz M. , Asiri Ibrahim M. , Alshayban Dhfer M. , Alsultan Mohammed M. , Alshehail Bashayer , Almalki Bassem A. , Hussein Dania , Alotaibi Mansour M. , Alfayez Osamah M.
Published: January 2024

This study examined the level of adherence to self-care behaviors among individuals with type 2 diabetes in Saudi Arabia and its connection with depression and demographic factors. A cross-sectional survey was conducted among diabetes patients using the Patient Health Questionnaire (PHQ-9) to measure depression and the Summary of Diabetes Self-Care Activities (SDSCA) to evaluate diabetes self-care activities. Among the 252 participants who completed the survey, 43.2% were older than 55 and 59% were men. The ordinal regression model showed an association between the PHQ-9 and SDSCA scores with an OR of 0.83 (95% CI: 0.71 to 0.96, p = 0.013). The PHQ-9 score was significantly associated with blood sugar monitoring (OR: 0.90 [95% CI: 0.82 to 0.99, p = 0.003]), exercise (OR: 0.88 [95% CI: 0.79 to 0.98, p = 0.002]), and diet (OR: 0.94 [95% CI: 0.85 to 1.03, p = 0.045]). Of all the diabetes-related factors, only a history of hospitalization and receiving diabetes education were found to be associated with improved self-care behaviors. In conclusion, a negative association was found between PHQ-9 scores and the SDSCA mean score and most daily diabetic self-care behavior components.
Overview of COVID-19 Infection, Treatment, and Prevention in Children
Authors: Kao Carol M.
Published: January 2024

Coronavirus disease 2019 (COVID-19), caused by the novel respiratory virus—severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)—was declared a global pandemic by the World Health Organization on 11 March 2020. Since then, substantial gains have been made in our understanding of COVID-19 epidemiology, disease presentation, and management. While children tend to have less severe disease courses compared to adults, children can still develop severe COVID-19 infections, particularly in those with underlying medical conditions such as obesity, chronic lung disease, or prematurity. In addition, children are at risk of severe complications of COVID-19 infection, such as multisystem inflammatory syndrome in children (MIS-C) or long COVID. The case definitions of MIS-C and long COVID have continued to evolve with the increased understanding of these new entities; however, improved methods of diagnosis and determination of the optimal management are still needed. Furthermore, with the continued circulation of SARS-CoV-2 variants, there remains a need for clinicians to remain up-to-date on the latest treatment and prevention options. The purpose of this review is to provide an evidence-based review of what we have learned about COVID-19 in children since the start of the pandemic and how best to counsel children and their families on the best methods of prevention.
Muscle Strength and Male Sexual Function
Authors: Viken Anders Flataker , Siiak Silver Peeter , Schlünssen Vivi , Thorarinsdottir Elin Helga , Skulstad Svein Magne , Gyawali Sanjay , Bertelsen Randi Jacobsen , Real Francisco Gómez
Published: January 2024

Sexual dysfunction, in particular erectile dysfunction, is a common complaint among aging men. Obesity, diabetes, hypertension, and smoking are shown to be independent risk factors for erectile dysfunction, while cardiorespiratory fitness is shown to be protective. Less is known about the role of muscle strength in male sexual function. Our objective was to study the association between male sexual function and typical cardiovascular risk factors, together with exercise and muscle strength. We included data from the fourth wave of the RHINE study. Data on anthropometrics, exercise habits, diseases, muscle strength, and sexual function were collected using questionnaires, including the Aging Males’ Symptoms (AMS) scale. We used multivariable logistic regression analysis to measure the association between sexual function and body mass index (BMI), age, smoking, diabetes, hypertension, exercise and muscle strength status. We included 2116 men aged 48–75 from four Nordic-Baltic countries. BMI, age, smoking, diabetes, and hypertension were found to be associated with higher odds of reporting decreased sexual function, while reporting intact muscle strength was associated with lower odds. In a large Nordic-Baltic male study population, we show that known cardiovascular risk factors are associated with decreased sexual function, while reporting intact muscle strength is associated with lower odds of reporting decreased sexual function.
Diagnostic Infrared Thermography of the Tongue and Taste Perception in Patients with Oral Lichen Planus: Case-Control Study
Authors: Nicolas-Rodriguez Elena , Pons-Fuster Eduardo , López-Jornet Pia
Published: January 2024

Oral lichen planus (OLP) is a chronic inflammatory disease of autoimmune origin that affects the skin and mucous membranes. The aim of this study was to assess the effectiveness of infrared thermography (IRT) as a diagnostic tool in patients with oral lichen planus (OLP), as well as disturbances in taste perception, in cases with and without tongue involvement. A case-control study was carried out in a sample of 87 patients divided into three groups: healthy individuals (controls; n = 43), OLP patients without involvement of the tongue (n = 24), and OLP patients with involvement of the tongue (n = 20). The patient symptoms and the clinical characteristics of the lesions were assessed. Four thermal images of the tongue were obtained using IRT: dorsum, right and left lateral surface, and tip of the tongue. General taste perception capacity and subjective sweet, acid, salty, and bitter sensation were evaluated in all three groups. There were no statistically significant differences in the IRT values among the three groups (p ≥ 0.05). In the OLP patients with involvement of the tongue, significantly higher values were observed in the mixed forms of the disease (p = 0.032). The OLP patients with and without involvement of the tongue showed no significant alterations in taste sensation (p = 0.69). IRT may serve as a complementary tool for assessing the activity of OLP with involvement of the tongue. However, more research is needed in this field.
Prediction of the Bond Strength of Externally Bonded FRP Sheets Applied to Concrete via Grooves Technique Using Artificial Neural Networks
Authors: Salmi Abdelatif
Published: January 2024

The present study aims to fill a gap in the literature on the estimation of the bond strength of fiber reinforced polymer sheets bonded to concrete, via the externally bonded reinforcement on grooves (EBROG) technique, employing the curve-fitting on existing datasets in the literature and the methodology of Artificial Neural Networks (ANNs). Therefore, a dataset of 39 experimental results derived from EBROG technique is collected from the literature. A mathematical equation for the bond strength of FRP sheets applied on concrete via the EBROG technique was suggested using curve-fitting and general regression. The proposed mathematical equation is compared and validated with experimental results. The developed ANN model was constructed after testing diverse hidden layers and neurons to find the optimal predictions. The validation of the model is carried out using the experimental results and a statistical analysis is applied to assess the proposed mathematical equation and the proposed ANN model. Furthermore, a parametric study using the ANN model was also performed to investigate the influence of various factors on the bond strength of FRP sheets bonded to concrete. The parametric study proves that the bond strength increases with increasing the tensile stiffness per width, the FRP sheet width, and the concrete compressive strength; however, the effect of the Groove’s width and depth is found to be not monotonous.
Deep Learning Applications in Vessel Dead Reckoning to Deal with Missing Automatic Identification System Data
Authors: Sedaghat Atefe , Arbabkhah Homayoon , Jafari Kang Masood , Hamidi Maryam
Published: January 2024

This research introduces an online system for monitoring maritime traffic, aimed at tracking vessels in water routes and predicting their subsequent locations in real time. The proposed framework utilizes an Extract, Transform, and Load (ETL) pipeline to dynamically process AIS data by cleaning, compressing, and enhancing it with additional attributes such as online traffic volume, origin/destination, vessel trips, trip direction, and vessel routing. This processed data, enriched with valuable details, serves as an alternative to raw AIS data stored in a centralized database. For user interactions, a user interface is designed to query the database and provide real-time information on a map-based interface. To deal with false or missing AIS records, two methods, dead reckoning and machine learning techniques, are employed to anticipate the trajectory of the vessel in the next time steps. To evaluate each method, several metrics are used, including R squared, mean absolute error, mean offset, and mean offset from the centerline. The functionality of the proposed system is showcased through a case study conducted in the Gulf Intracoastal Waterway (GIWW). Three years of AIS data are collected and processed as a simulated API to transmit AIS records every five minutes. According to our results, the Seq2Seq model exhibits strong performance (0.99 R squared and an average offset of ~1400 ft). However, the second scenario, dead reckoning, proves comparable to the Seq2Seq model as it involves recalculating vessel headings by comparing each data point with the previous one.
Evaluation of Cytotoxic and Genotoxic Effects in Buccal Mucosal Cells in Non-Smokers and Users of Traditional Combustible Tobacco Products and Non-Combustible Alternatives
Authors: Tadin Antonija , Stazic Vinka , Galic Nada , Zeljezic Davor
Published: January 2024

Aims/Objectives: The aim of this cross-sectional observational study was to investigate cytogenetic damage to the buccal mucosa in non-smokers and consumers of traditional combustible tobacco products and non-combustible alternatives. Methods: A total of 160 participants were divided into four groups according to the type of product used, including non-smokers, users of conventional combustible tobacco (cigarettes), heated tobacco, and electronic, tobacco-free vapor products (e-cigarettes). Buccal mucosa samples were analyzed using the micronucleus cytome assay to assess cytotoxic and genotoxic damage. Results: E-cigarette users showed significantly higher values for all tested parameters in the micronucleus test compared to non-smokers (p < 0.05). Similarly, users of tobacco heating products showed an increase in all parameters (p < 0.05), with the exception of the number of cells with micronuclei. Conventional cigarette smokers showed a notable increase in the number of binucleated cells and cells with karyorrhexis and karyolysis (p ≤ 0.05). When assessing the differences between users of traditional combustible tobacco products and non-combustible alternatives, these did not appear to be significant, except for e-cigarette users, who had significantly more cells with condensed chromatin (p ≤ 0.001), while users of tobacco heating products had more pyknotic cells (p ≤ 0.001). Conclusion: The results of this study underscore the heightened occurrence of cytotoxic and genotoxic damage in users of both conventional combustible tobacco products and non-combustible alternatives compared to non-smokers, emphasizing the detrimental impact of these products on the oral mucosa.
Immunotherapy Combined with Radiation in Malignant Melanoma without BRAF Mutations Brain Metastases—Favorable Response after Immunotherapy Continued beyond Progression
Authors: Rahnea-Nita Roxana-Andreea , Rebegea Laura-Florentina , Toma Radu-Valeriu , Mocanu Horia , Soare Ioana , Mihailov Raul , Nechifor Alexandru , Guliciuc Mădălin , Constantin Georgiana Bianca , Rahnea-Nita Gabriela
Published: January 2024

We present the case of a patient who was diagnosed in 2018 with nodular Malignant Melanoma (MM) without BRAF V 600 mutations stage 3 C (pT4b pN1a M0), and who underwent adjuvant citokines treatment with Interferon alpha 2b-48 weeks. Immunotherapy was initiated in January 2021 for lung and lymph node metastases. In June 2021, there was a partial response of the lung and lymph node metastases, but there was also progression to brain metastases. Immunotherapy was continued and Whole Brain Radiotherapy (WBRT) was performed. In September 2023, the imaging investigations revealed a favorable response, with no lesions suggestive of secondary determinations. The combination of Radiotherapy (RT) and Immunotherapy (IT) with Immune Checkpoint Inhibitors (ICI) has an abscopal effect. There is a coordinated action in the combination of RT and IT in order to obtain a common result, with the antitumor effect being greater than if RT or IT acted separately.
Histopathological Clues of Enhanced Inflammation in the Placental Tissue of Women with Chronic Venous Disease in Lower Limbs during Pregnancy
Authors: Sánchez-Gil María Asunción , Fraile-Martinez Oscar , García-Montero Cielo , Toledo María Del Val , Guijarro Luis G. , De León-Luis Juan A. , Bravo Coral , Díaz-Pedrero Raúl , López-Gonzalez Laura , Saez Miguel A. , Álvarez-Mon Melchor , García-Honduvilla Natalio , Ortega Miguel A.
Published: January 2024

It is estimated that approximately one in three women develop chronic venous disease (CVD) during pregnancy, a broad spectrum of morphofunctional disorders affecting the venous system in different regions of the body, including the lower limbs. A growing body of evidence supports the diverse maternofetal consequences derived from this condition, with the placenta being an organ particularly affected. Among other consequences, having CVD during pregnancy has been associated with systemic inflammation and altered cytokines and chemokine profiles in the maternal and fetal serum related to this condition. In the present work, we aimed to analyze if these inflammatory changes also occurred in the placental tissue of women with CVD, exploring by immunohistochemistry and real-time PCR (RT-qPCR) gene and protein expression of critical inflammatory markers like allograft inflammatory factor 1 (AIF-1), interleukin 10 (IL-10), IL-12A, and IL-18. Our results demonstrate an enhanced tissue expression of AIF-1, IL-12A, and IL-18, accompanied by a decrease in IL-10 in the placentas of women who had undergone CVD during pregnancy. Overall, our results suggest a possible pathophysiological role of inflammation in the placental tissue of women with CVD during pregnancy, although the precise consequences of this feature remain to be deeply analyzed.
Experiences Using Ethereum and Quorum Blockchain Smart Contracts in Dairy Production
Authors: Melissari Filisia , Papadakis Andreas , Chatzitheodorou Dimitris , Tran Duc , Schouteten Joachim , Athanasiou Georgia , Zahariadis Theodore
Published: January 2024

feta cheese is a Greek protected designation of origin (PDO) product that is produced in three main phases: milk collection, cheese preparation and maturation, and product packaging. Each phase must be aligned with quantitative rules, stemming from the legislation framework and best practices. The production complexity, the increased production cost, centralised and monolithic traceability systems, and the lack of a systematic monitoring framework have made dairy products a commodity with increased frequency of food fraud. Given the context of the dairy section in Greece, this study aims to examine (a) whether it is possible to model the end-to-end process of PDO feta cheese considering production rules to develop a trustworthy blockchain-based traceability system (b) how to associate the (‘easy-to-retrieve’, operational) traceability data with the (difficult-to-assess) product characteristics meaningful to the consumer, (c) how to design a technical solution ensuring that information is accessible by the stakeholders and the consumer, while minimising blockchain-related delay, and (d) how to design a graphical user interface and offer tools to consumers so that traceability information is communicated effectively and they can verify it through access to the blockchain. In terms of methods, we analyse and model the process steps, identify measurable, operational parameters and translate the legislative framework into rules. These rules are designed and codified as blockchain smart contracts that ensure the food authenticity and compliance with legislation. The blockchain infrastructure consists of the private Quorum blockchain that is anchored to the public infrastructure of Ethereum. Mechanisms to address scalability in terms of dynamic data volumes, effective data coding, and data verification at the edge as well as relevant limitations are discussed. Consumers are informed about traceability information by using QR codes on food packaging and can verify the data using the blockchain tools and services.
On the Acquisition of Differential Object Marking in Child Heritage Spanish: Bilingual Education, Exposure, and Age Effects (In Memory of Phoebe Search)
Authors: Thane Patrick D.
Published: January 2024

Studies on school-aged children have been infrequent in research on Spanish as a heritage language. The present study explored how dual-language immersion education, patterns of heritage language use, proficiency, and age shape child Spanish heritage speakers’ production and selection of differential object marking (DOM). A total of 57 English–Spanish bilingual children and 18 Spanish-dominant adults completed sentence completion and morphology selection tasks. Results revealed that the group of heritage speaker children that produced and selected the differential object marker most frequently was the seventh and eighth grade children (ages 12–14, the oldest in the study) who had completed a dual-language immersion program. Different factors accounted for variability in each task: bilingual education and proficiency affected the production of DOM, while age affected selection. Heritage speakers selected DOM more frequently than they produced this structure. These findings have implications for theories of heritage language acquisition that emphasizes that language experience and exposure account for differences between heritage speakers and argue for the dissociation of production from underlying syntactic knowledge. The data also argue that heritage speakers may possess a bilingual alignment for DOM, whereby underlying receptive knowledge is modulated by cumulative exposure, while production depends more on bilingual education and proficiency in Spanish.
Simple and Effective Squash-PCR for Rapid Genotyping of Industrial Microalgae
Authors: Yuan Guoliang , Gao Song , Czajka Jeffrey J. , Dai Ziyu , Pomraning Kyle R. , Duong Rylan D. , Hofstad Beth A. , Deng Shuang
Published: January 2024

Microalgae are recognized for their versatility in providing renewable energy, biopharmaceuticals, and nutraceuticals, attributed to their sustainable, renewable, and cost-effective nature. Genetic engineering has proven highly effective in enhancing microalgae production. PCR-based genotyping is the primary method for screening genetically transformed microalgae cells. Recently, we developed a novel PCR method, namely Squash-PCR, and employed it for the molecular analysis of industrially important fungi and yeasts. In this study, we successfully implemented the Squash-PCR technique in 12 industrially significant algae species. This approach offers a quick and reliable means of obtaining DNA templates directly from squashed algal cells, eliminating the need for time-consuming and labor-intensive cultivation and genomic DNA extraction steps. Our results demonstrate the effectiveness of Squash-PCR in detecting and characterizing target genes of interest in 12 different algae species. Overall, this study establishes the Squash-PCR method as a valuable tool for molecular studies in algae, enabling researchers to rapidly screen and manipulate genetic traits in diverse algal species.
Performance Evaluation of Retail Warehouses: A Combined MCDM Approach Using G-BWM and RATMI
Authors: Barasin Abdullah M. , Alqahtani Ammar Y. , Makki Anas A.
Published: January 2024

Background: The retail sector has experienced significant growth in recent years, necessitating efficient supply chain management and sustainable logistics practices. Evaluating the performance of retail warehouses is crucial for meeting customer expectations and enhancing operational efficiency. Methods: This study employed a combined multi-criteria decision-making (MCDM) approach, using the group best–worst method (G-BWM) for weighting criteria and ranking the alternatives based on the trace-to-median index (RATMI) for warehouse ranking. The performance criteria were cost, quality, time, productivity, and safety. Data were collected from four mega retail warehouses in the western region of Saudi Arabia for evaluation and analysis. Results: The evaluation of retail warehouse performance using the MCDM approach provided valuable insights for decision-makers and warehouse experts. The criteria weights were determined using the G-BWM, and the RATMI enabled the ranking of the warehouses based on their weighted performance scores. The results highlight the strengths and weaknesses of each warehouse, facilitating strategic planning, resource allocation, and operational improvements. Conclusions: This study presents a novel combined MCDM performance evaluation approach for retail warehouses. The study has implications for effective decision-making processes, resource allocation, and operational efficiency. Furthermore, it serves as a foundation for future research, exploring additional dimensions of warehouse performance and enabling sustainable logistics within the broader supply chain context.
Effect of Magnetic Field on Maskless Localized Electrodepositing Three-Dimensional Microstructure of Nano Nickel Crystals
Authors: Wu Menghua , Jiang Bingchun , Xiao Yuqing , Jia Weiping
Published: January 2024

In the intricate process of maskless localized electrodeposition (MLED) for fabricating three-dimensional microstructures, specifically nickel micro-columns with an aspect ratio of 7:1, magnetic fields of defined strength were employed, oriented both parallel and anti-parallel to the electric field. The aim was to achieve nanocrystalline microstructures and elevated deposition rates. A detailed comparative analysis was conducted to examine the volumetric deposition rate, surface morphology, and grain size of the MLED nickel crystal 3D microstructures, both in the absence and presence of the two magnetic field directions, facilitated by a self-assembled experimental setup. The results indicate that the anti-parallel magnetic field significantly boosts the volumetric deposition rate to a notable 19,050.65 μm3/s and refines the grain size, achieving an average size of 24.82 nm. Conversely, the parallel magnetic field is found to enhance the surface morphology of the MLED nickel crystal 3D microstructure.
Hematite (α-Fe2O3) with Oxygen Defects: The Effect of Heating Rate for Photocatalytic Performance
Authors: Sakamoto Masanori , Fujita Ryoga , Nishikawa Masami , Hirazawa Hideyuki , Ueno Yuichi , Yamamoto Manami , Takaoka Suzu
Published: January 2024

Hematite (α-Fe2O3) emerges as an enticing material for visible-light-driven photocatalysis owing to its remarkable stability, low toxicity, and abundance. However, its inherent shortcomings, such as a short hole diffusion length and high recombination rate, hinder its practical application. Recently, oxygen vacancies (Vo) within hematite have been demonstrated to modulate its photocatalytic attributes. The effects of Vo can be broadly categorized into two opposing aspects: (1) acting as electron donors, enhancing carrier conductivity, and improving photocatalytic performance and (2) acting as surface carrier traps, accelerating excited carrier recombination, and deteriorating performance. Critically, the generation rate, distribution, role, and behavior of Vo significantly differ for synthesis methods due to differences in formation mechanisms and oxygen diffusion. This complexity hampers simplified discussions of Vo, necessitating careful investigation and nuanced discussion tailored to the specific method and conditions employed. Among various approaches, hydrothermal synthesis offers a simple and cost-effective route. Here, we demonstrate a hydrothermal synthesis method for Vo introduction to hematite using a carbon source, where variations in the heating rate have not been previously explored in terms of their influence on Vo generation. The analyses revealed that the concentration of Vo was maximized at a heating rate of 16 °C/min, indicative of a high density of surface defects. With regard to photocatalytic performance, elevated heating rates (16 °C/min) fostered the formation of Vo primarily on the hematite surface. The photocatalytic activity was 7.1 times greater than that of the sample prepared at a low heating rate (2 °C/min). These findings highlight the crucial role of surface defects, as opposed to bulk defects, in promoting hematite photocatalysis. Furthermore, the facile control over Vo concentration achievable via manipulating the heating rate underscores the promising potential of this approach for optimizing hematite photocatalysts.
Analysis of the Control Characteristics of the Electro-Hydraulic Vibration System Based on the Single-Neuron Control Algorithm
Authors: Jia Wenang , Chen Zeji , Chen Tongzhong , Li Sheng
Published: January 2024

This paper proposes an electro-hydraulic vibration control system based on the single-neuron PID algorithm, which improves the operating frequency of the electro-hydraulic fatigue testing machine and the control accuracy of the load force. Through mathematical modeling of the electro-hydraulic vibration system (EVS), a MATLAB/Simulink simulation, and experimental testing, this study systematically analyzes the output waveform of the EVS as well as the closed-loop situation of load force amplitude and offset under the action of the single-neuron PID algorithm. The results show that: the EVS with a 2D vibration valve as the core, which can control the movement of the spool in the two-degrees-of-freedom direction, can realize the output of an approximate sinusoidal load force waveform from 0 to 800 Hz. The system controlled by the single-neuron PID algorithm is less complex to operate than the traditional PID algorithm. It also has a short rise time for the output load force amplitude curve and a maximum control error of only 1.2%. Furthermore, it exhibits a rapid closed-loop response to the load force offset. The range variability of the load force is measured to be 1.43%. A new scheme for the design of EVS is provided in this study, which broadens the application range of electro-hydraulic fatigue testing machines.
Nonlinear Dynamics and Combination Resonance of a Flexible Turbine Blade with Contact and Friction of Shrouds
Authors: Li Hua , Yuan Gaofei , Yu Zifeng , Wang Yuefang , Marmysh Dzianis
Published: January 2024

Flexible shrouded blades are commonly adopted in the last stages of steam turbines where complicated dynamical behavior can be induced by dry friction force generated on contacting interfaces between adjacent shrouds and the geometric nonlinearity due to the structural flexibility of the blades. In this paper, combination resonance caused by contact and friction forces generated on shroud interfaces is investigated, which is a concurrence of 1:3 internal resonance involving the first and second modes in the flapwise direction and the primary resonance of the first flapwise mode. The stiffness and damping at the contact interface are obtained by linearizing the contact and friction forces between shrouds through the harmonic balance method. The vibrating blade is modeled as a beam with a concentrated mass of which the responses under the combination resonance are solved through the multiple-scale method. Sensitivities of response with respect to the angle of shrouds, contact stiffness and rotation speed are illustrated, and the influences of these parameters on the periodicity and amplitudes of steady responses are demonstrated. The parametric regions where the combination resonance occurs are pointed out. Finally, parametric analyses are presented to show how the amplitude–frequency relation of the multiple-scale solutions under the combination resonance vary with detuning and design parameters. The present research provides a designing basis for improving the dynamic performance of flexible shrouded blades and suppressing vibrations of blades by adjusting structural parameters in practical engineering.