bluuebunny/crossref_metadata_2025 huggingface dataset:
Bovine Lactoferrin Pre-Treatment Induces Intracellular Killing of AIEC LF82 and Reduces Bacteria-Induced DNA Damage in Differentiated Human Enterocytes
Authors: Lepanto Maria Stefania , Rosa Luigi , Cutone Antimo , Scotti Mellani Jinnett , Conte Antonietta Lucia , Marazzato Massimiliano , Zagaglia Carlo , Longhi Catia , Berlutti Francesca , Musci Giovanni , Valenti Piera , Conte Maria Pia
Published: November 2019

LF82, a prototype of adherent-invasive E. coli (AIEC), is able to adhere to, invade, survive and replicate into intestinal epithelial cells. LF82 is able to enhance either its adhesion and invasion by up-regulating carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM-6), the main cell surface molecule for bacterial adhesion, and its intracellular survival by inducing host DNA damage, thus blocking the cellular cycle. Lactoferrin (Lf) is a multifunctional cationic glycoprotein of natural immunity, exerting an anti-invasive activity against LF82 when added to Caco-2 cells at the moment of infection. Here, the infection of 12 h Lf pre-treated Caco-2 cells was carried out at a time of 0 or 3 or 10 h after Lf removal from culture medium. The effect of Lf pre-treatment on LF82 invasiveness, survival, cell DNA damage, CEACAM-6 expression, apoptosis induction, as well as on Lf subcellular localization, has been evaluated. Lf, even if removed from culture medium, reduced LF82 invasion and survival as well as bacteria-induced DNA damage in Caco-2 cells independently from induction of apoptosis, modulation of CEACAM-6 expression and Lf sub-cellular localization. At our knowledge, this is the first study showing that the sole Lf pre-treatment can activate protective intracellular pathways, reducing LF82 invasiveness, intracellular survival and cell–DNA damages.
Perinatal Use of Melatonin for Offspring Health: Focus on Cardiovascular and Neurological Diseases
Authors: Hsu Chien-Ning , Huang Li-Tung , Tain You-Lin
Published: November 2019

Cardiovascular and neurological diseases can originate in early life. Melatonin, a biologically active substance, acts as a pleiotropic hormone essential for pregnancy and fetal development. Maternal melatonin can easily pass the placenta and provide photoperiodic signals to the fetus. Though melatonin uses in pregnant or lactating women have not yet been recommended, there is a growing body of evidence from animal studies in support of melatonin as a reprogramming strategy to prevent the developmental programming of cardiovascular and neurological diseases. Here, we review several key themes in melatonin use in pregnancy and lactation within offspring health and disease. We have particularly focused on the following areas: the pathophysiological roles of melatonin in pregnancy, lactation, and fetal development; clinical uses of melatonin in fetal and neonatal diseases; experimental evidence supporting melatonin as a reprogramming therapy to prevent cardiovascular and neurological diseases; and reprogramming mechanisms of melatonin within developmental programming. The targeting of melatonin uses in pregnancy and lactation will be valuable in the prevention of various adult chronic diseases in later life, and especially cardiovascular and neurological diseases.
Recovery in the Myogenic Program of Congenital Myotonic Dystrophy Myoblasts after Excision of the Expanded (CTG)n Repeat
Authors: André Laurène M. , van Cruchten Remco T.P. , Willemse Marieke , Bezstarosti Karel , Demmers Jeroen A.A. , van Agtmaal Ellen L. , Wansink Derick G. , Wieringa Bé
Published: November 2019

The congenital form of myotonic dystrophy type 1 (cDM) is caused by the large-scale expansion of a (CTG•CAG)n repeat in DMPK and DM1-AS. The production of toxic transcripts with long trinucleotide tracts from these genes results in impairment of the myogenic differentiation capacity as cDM’s most prominent morpho-phenotypic hallmark. In the current in vitro study, we compared the early differentiation programs of isogenic cDM myoblasts with and without a (CTG)2600 repeat obtained by gene editing. We found that excision of the repeat restored the ability of cDM myoblasts to engage in myogenic fusion, preventing the ensuing myotubes from remaining immature. Although the cDM-typical epigenetic status of the DM1 locus and the expression of genes therein were not altered upon removal of the repeat, analyses at the transcriptome and proteome level revealed that early abnormalities in the temporal expression of differentiation regulators, myogenic progression markers, and alternative splicing patterns before and immediately after the onset of differentiation became normalized. Our observation that molecular and cellular features of cDM are reversible in vitro and can be corrected by repeat-directed genome editing in muscle progenitors, when already committed and poised for myogenic differentiation, is important information for the future development of gene therapy for different forms of myotonic dystrophy type 1 (DM1).
3-(3,4-Dimethoxyphenyl)-5-(2-fluorophenyl)-1-phenyl-4,5-dihydro-1H-pyrazole
Authors: Zamri Adel , Teruna Hilwan Y. , Wulansari Sri , Herfindo Noval , Frimayanti Neni , Ikhtiarudin Ihsan
Published: November 2019

A new analogue of fluorinated pyrazoline (compound 1) has been synthesized via one-pot three-component reaction in a sealed-vessel reactor, Monowave 50. The structure of compound 1 has been established by spectroscopy analysis, including UV, FT-IR, HRMS, 1H and 13C NMR spectroscopy. Based on the in silico studies, this compound showed a good potential as an inhibitor for dengue virus type 2 (DEN2) NS2B/NS3 serine protease and can be used as a reference in the next design of an antidengue virus.
The Performance of Carbonation-Cured Concrete
Authors: Li Zhen , He Zhen , Chen Xiaorun
Published: November 2019

The research shows that carbonation-cured concrete has several mechanical and durability properties that are better than those of moisture-cured concrete. However, many properties of carbonation-cured concrete have not yet been studied. In this research, carbonation-cured concrete was prepared by pre-curing, carbonation curing, and then moisture curing. The compressive strength, CO2 uptake, pH value, chloride ion permeability and abrasion resistance of the carbonation-cured concrete were investigated. Results showed that the compressive strength of carbonation-cured concrete was more than 10% higher than that of moisture-cured concrete at the same age; a steel bar is stable in carbonation-cured concrete; and carbonation-cured concrete exhibited better abrasion resistance and chloride ion permeability than that of moisture-cured concrete. The optimization of pore structure and improvement in the micro-hardness are the reasons for the improved chloride ion permeability and abrasion resistance of carbonation-cured concrete.
Reddish-Orange Luminescence from BaF2:Eu3+ Fluoride Nanocrystals Dispersed in Sol-Gel Materials
Authors: Pawlik Natalia , Szpikowska-Sroka Barbara , Pisarska Joanna , Goryczka Tomasz , Pisarski Wojciech A.
Published: November 2019

Nanocrystalline transparent BaF2:Eu3+ glass-ceramic materials emitting reddish-orange light were fabricated using a low-temperature sol-gel method. Several experimental techniques were used to verify structural transformation from precursor xerogels to sol-gel glass-ceramic materials containing fluoride nanocrystals. Thermal degradation of xerogels was analyzed by thermogravimetric analysis (TG) and differential scanning calorimetry method (DSC). The presence of BaF2 nanocrystals dispersed in sol-gel materials was confirmed by the X-ray diffraction (XRD) analysis and transmission electron microscopy (TEM). In order to detect structural changes in silica network during annealing process, the infrared spectroscopy (IR-ATR) was carried out. In particular, luminescence spectra of Eu3+ and their decays were examined in detail. Some spectroscopic parameters of Eu3+ ions in glass-ceramics containing BaF2 nanocrystals were determined and compared to the values obtained for precursor xerogels. It was observed, that the intensities of two main red and orange emission bands corresponding to the 5D0→7F2 electric-dipole transition (ED) and the 5D0→7F1 magnetic-dipole (MD) transition are changed significantly during transformation from xerogels to nanocrystalline BaF2:Eu3+ glass-ceramic materials. The luminescence decay analysis clearly indicates that the measured lifetime 5D0 (Eu3+) considerably enhanced in nanocrystalline BaF2:Eu3+ glass-ceramic materials compared to precursor xerogels. The evident changes in luminescence spectra and their decays suggest the successful migration of Eu3+ ions from amorphous silica network to low-phonon BaF2 nanocrystals.
Ag Nanoparticle-Decorated Oxide Coatings Formed via Plasma Electrolytic Oxidation on ZrNb Alloy
Authors: Oleshko Oleksandr , Deineka V Volodymyr , Husak Yevgeniia , Korniienko Viktoriia , Mishchenko Oleg , Holubnycha Viktoriia , Pisarek Marcin , Michalska Joanna , Kazek-Kęsik Alicja , Jakóbik-Kolon Agata , Simka Wojciech , Pogorielov Maksym
Published: November 2019

Plasma electrolytic oxidation (PEO) can provide an ideal surface for osteogenic cell attachment and proliferation with further successful osteointegration. However, the same surface is attractive for bacteria due to similar mechanisms of adhesion in prokaryotic and eukaryotic cells. This issue requires the application of additional surface treatments for effective prevention of postoperative infectious complications. In the present work, ZrNb alloy was treated in a Ca-P solution with Ag nanoparticles (AgNPs) for the development of a new oxide layer that hosted osteogenic cells and prevented bacterial adhesion. For the PEO, 0.5 M Ca(H2PO2)2 solution with 264 mg L−1 of round-shaped AgNPs was used. Scanning electron microscopy with energy-dispersive x-ray and x-ray photoelectron spectroscopy were used for morphology and chemical analysis of the obtained samples; the SBF immersion test, bacteria adhesion test, and osteoblast cell culture were used for biological investigation. PEO in a Ca-P bath with AgNPs provides the formation of a mesoporous oxide layer that supports osteoblast cell adhesion and proliferation. Additionally, the obtained surface with incorporated Ag prevents bacterial adhesion in the first 6 h after immersion in a pathogen suspension, which can be an effective approach to prevent infectious complications after implantation.
Mycosporine-Like Amino Acids: Making the Foundation for Organic Personalised Sunscreens
Authors: Rosic Nedeljka
Published: November 2019

The surface of the Earth is exposed to harmful ultraviolet radiation (UVR: 280–400 nm). Prolonged skin exposure to UVR results in DNA damage through oxidative stress due to the production of reactive oxygen species (ROS). Mycosporine-like amino acids (MAAs) are UV-absorbing compounds, found in many marine and freshwater organisms that have been of interest in use for skin protection. MAAs are involved in photoprotection from damaging UVR thanks to their ability to absorb light in both the UV-A (315–400 nm) and UV-B (280–315 nm) range without producing free radicals. In addition, by scavenging ROS, MAAs play an antioxidant role and suppress singlet oxygen-induced damage. Currently, there are over 30 different MAAs found in nature and they are characterised by different antioxidative and UV-absorbing capacities. Depending on the environmental conditions and UV level, up- or downregulation of genes from the MAA biosynthetic pathway results in seasonal fluctuation of the MAA content in aquatic species. This review will provide a summary of the MAA antioxidative and UV-absorbing features, including the genes involved in the MAA biosynthesis. Specifically, regulatory mechanisms involved in MAAs pathways will be evaluated for controlled MAA synthesis, advancing the potential use of MAAs in human skin protection.
Effects of a Novel Adsorbent on Membrane Fouling by Natural Organic Matter in Drinking Water Treatment
Authors: Manamperuma Lelum , Vik Eilen , Benjamin Mark , Cai Zhenxiao , Skjefstad Jostein
Published: November 2019

Irreversible fouling of water filtration membranes reduces filter longevity and results in higher costs associated with membrane maintenance and premature replacement. The search for effective pretreatment methods to remove foulants that tend to irreversibly foul membranes is ongoing. In this study, a novel adsorbent (Heated Aluminum Oxide Particles (HAOPs)) was deployed in a fully automated pilot system to remove natural organic matter (NOM) from the surface water source used at the UniVann water treatment plant (WTP) in Ullensaker County, Norway. The pilot plant treatment process consists of passing the water through a thin layer of HAOPs that has been deposited on a mesh support. The HAOPs layer acts as an active packed bed which removes NOM from the water. Fluxes around 120 L/m2/h (LMH) at transmembrane pressure (TMP) below 10.7 psi (0.7 bar) were achieved over production cycles excessing 12 h. Treatment achieved always >85% colour removal and effluent colour <5 mg Pt/L (the target of treatment), and always <0.01 NTU turbidity and non-detectable suspended solids in the permeate. The HAOPs mixture after saturated with NOM is easy to remove by disruption of the HAOPs by rinsing the mesh surface, and the sludge is easily dewatered to higher of dry solids content.
Historical Biobanks in Breast Cancer Metabolomics— Challenges and Opportunities
Authors: Madssen Torfinn S. , Cao Maria D. , Pladsen Arne V. , Ottestad Lars , Sahlberg Kristine K. , Bathen Tone F. , Giskeødegård Guro F.
Published: November 2019

Background: Metabolomic characterization of tumours can potentially improve prediction of cancer prognosis and treatment response. Here, we describe efforts to validate previous metabolomic findings using a historical cohort of breast cancer patients and discuss challenges with using older biobanks collected with non-standardized sampling procedures. Methods: In total, 100 primary breast cancer samples were analysed by high-resolution magic angle spinning magnetic resonance spectroscopy (HR MAS MRS) and subsequently examined by histology. Metabolomic profiles were related to the presence of cancer tissue, hormone receptor status, T-stage, N-stage, and survival. RNA integrity number (RIN) and metabolomic profiles were compared with an ongoing breast cancer biobank. Results: The 100 samples had a median RIN of 4.3, while the ongoing biobank had a significantly higher median RIN of 6.3 (p = 5.86 × 10−7). A low RIN was associated with changes in choline-containing metabolites and creatine, and the samples in the older biobank showed metabolic differences previously associated with tissue degradation. The association between metabolomic profile and oestrogen receptor status was in accordance with previous findings, however, with a lower classification accuracy. Conclusions: Our findings highlight the importance of standardized biobanking procedures in breast cancer metabolomics studies.
Investigation of Molecular Details of Keap1-Nrf2 Inhibitors Using Molecular Dynamics and Umbrella Sampling Techniques
Authors: Londhe Ashwini Machhindra , Gadhe Changdev Gorakshnath , Lim Sang Min , Pae Ae Nim
Published: November 2019

In this study, we investigate the atomistic details of Keap1-Nrf2 inhibitors by in-depth modeling techniques, including molecular dynamics (MD) simulations, and the path-based free energy method of umbrella sampling (US). The protein–protein interaction (PPI) of Keap1-Nrf2 is implicated in several neurodegenerative diseases like cancer, diabetes, and cardiomyopathy. A better understanding of the five sub-pocket binding sites for Nrf2 (ETGE and DLG motifs) inside the Kelch domain would expedite the inhibitor design process. We selected four protein–ligand complexes with distinct co-crystal ligands and binding occupancies inside the Nrf2 binding site. We performed 100 ns of MD simulation for each complex and analyzed the trajectories. From the results, it is evident that one ligand (1VV) has flipped inside the binding pocket, whereas the remaining three were stable. We found that Coulombic (Arg483, Arg415, Ser363, Ser508, and Ser602) and Lennard–Jones (Tyr525, Tyr334, and Tyr572) interactions played a significant role in complex stability. The obtained binding free energy values from US simulations were consistent with the potencies of simulated ligands. US simulation highlight the importance of basic and aromatic residues in the binding pocket. A detailed description of the dissociation process brings valuable insight into the interaction of the four selected protein–ligand complexes, which could help in the future to design more potent PPI inhibitors.
Anti-Inflammatory Activities of Isogosferol, a Furanocoumarin Isolated from Citrus junos Seed Shells through Bioactivity-Guided Fractionation
Authors: Song Hwa Young , Jo Ara , Shin Jihun , Lim Eui Hyeon , Lee Ye Eun , Jeong Da Eun , Lee Mina
Published: November 2019

Citrus junos Tanaka is a traditional medicine for treating coughs, dyspepsia, diabetes, asthma, neuralgia, and inflammatory disorders, and is distributed in Asia, especially in Korea, Japan, and China. This study aimed to use bioactivity-guided fractionation to find therapeutic phytochemicals from C. junos seeds, which can attenuate inflammatory responses. Nine coumarins (1–9) were isolated from the methanolic extract of C. junos seed shells and the inhibitory effects against inflammatory mediators were investigated using murine macrophages. Among the coumarins, compound 3, isogosferol (ISO), more potently attenuated the production of nitric oxide (NO) in lipopolysaccharide (LPS)-induced RAW 264.7 cells. ISO also inhibited the expression of inducible nitric oxide (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated macrophages. Additionally, the phosphorylation of extracellular-regulated kinases (pERK)1/2 was reduced by ISO. We confirmed that ISO attenuated the release of interleukin-1 beta (IL-1β), which is a central mediator of the inflammatory response. These results demonstrate that ISO from C. junos seed shells may be a potent therapeutic candidate for the treatment of inflammatory diseases.
Raman Spectroscopy and Chemometric Modeling to Predict Physical-Chemical Honey Properties from Campeche, Mexico
Authors: Anguebes-Franseschi F. , Abatal M. , Pat Lucio , Flores A. , Córdova Quiroz A. V. , Ramírez-Elias M. A. , San Pedro L. , May Tzuc O. , Bassam A.
Published: November 2019

In this work, 10 chemometric models based on Raman spectroscopy were constructed to predict the physicochemical properties of honey produced in the state of Campeche, Mexico. The properties of honey studied were pH, moisture, total soluble solids (TSS), free acidity, lactonic acidity, total acidity, electrical conductivity, Redox potential, hydroxymethylfurfural (HMF), and ash content. These proprieties were obtained according to the methods described by the Association of Official Analytical Chemists, Codex Alimentarius, and the International Honey Commission. For the construction of the chemometric models, 189 honey samples were collected and analyzed in triplicate using Raman spectroscopy to generate the matrix data [X], which were correlated with each of the physicochemical properties [Y]. The predictive capacity of each model was determined by cross validation and external validation, using the statistical parameters: standard error of calibration (SEC), standard error of prediction (SEP), coefficient of determination of cross-validation (R2cal), coefficient of determination for external validation (R2val), and Student’s t-test. The statistical results indicated that the chemometric models satisfactorily predict the humidity, TSS, free acidity, lactonic acidity, total acidity, and Redox potential. However, the models for electric conductivity and pH presented an acceptable prediction capacity but not adequate to supply the conventional processes, while the models for predicting ash content and HMF were not satisfactory. The developed models represent a low-cost tool to analyze the quality of honey, and contribute significantly to increasing the honey distribution and subsequently the economy of the region.
Structural Analysis, Molecular Modelling and Preliminary Competition Binding Studies of AM-DAN as a NMDA Receptor PCP-Site Fluorescent Ligand
Authors: Ndzibongwana Sethu , Ngobese Samukelo , Sayed Ahmad , Shongwe Ciniso , White-Phillips Simon , Joubert Jacques
Published: November 2019

Excitotoxicity related to the dysfunction of the N-methyl-d-aspartate receptor (NMDAR) has been indicated to play an integral role in the pathophysiology of multiple disease states, including neurodegenerative disorders such as Parkinson’s disease. There is a notable gap in the market for novel NMDAR antagonists, however current methods to analyse potential antagonists rely on indirect measurements of calcium flux and hazardous radioligand binding assays. Recently, a fluorescent NMDAR ligand, N-adamantan-1-yl-dimethylamino-1-naphthalenesulfonic acid, known as AM-DAN was developed by our group. Additional studies on this ligand is necessary to evaluate its potential as a biological tool in NMDAR research. Therefore, this study was aimed at conducting structural analyses, fluorescence experiments, high-accuracy NMDAR molecular modelling and NMDAR phencyclidine (PCP) site competition binding studies using AM-DAN. Results revealed that AM-DAN has appropriate structural properties, significant fluorescent ability in various solvents and is able to bind selectively and compete for the PCP-binding site of the NMDAR. Therefore, AM-DAN holds promise as a novel fluorescent ligand to measure the affinity of prospective drugs binding at the NMDAR PCP-site and may circumvent the use of radioligands.
The Development of Novel Compounds Against Malaria: Quinolines, Triazolpyridines, Pyrazolopyridines and Pyrazolopyrimidines
Authors: C. S. Pinheiro Luiz , M. Feitosa Lívia , O. Gandi Marilia , F. Silveira Flávia , Boechat Nubia
Published: November 2019

Based on medicinal chemistry tools, new compounds for malaria treatment were designed. The scaffolds of the drugs used to treat malaria, such as chloroquine, primaquine, amodiaquine, mefloquine and sulfadoxine, were used as inspiration. We demonstrated the importance of quinoline and non-quinoline derivatives in vitro with activity against the W2 chloroquine-resistant (CQR) Plasmodium falciparum clone strain and in vivo against Plasmodium berghei-infected mouse model. Among the quinoline derivatives, new hybrids between chloroquine and sulfadoxine were designed, which gave rise to an important prototype that was more active than both chloroquine and sulfadoxine. Hybrids between chloroquine–atorvastatin and primaquine–atorvastatin were also synthesized and shown to be more potent than the parent drugs alone. Additionally, among the quinoline derivatives, new mefloquine derivatives were synthesized. Among the non-quinoline derivatives, we obtained excellent results with the triazolopyrimidine nucleus, which gave us prototype I that inspired the synthesis of new heterocycles. The pyrazolopyrimidine derivatives stood out as non-quinoline derivatives that are potent inhibitors of the P. falciparum dihydroorotate dehydrogenase (PfDHODH) enzyme. We also examined the pyrazolopyridine and pyrazolopyrimidine nuclei.
Grafting versus Crosslinking of Silk Fibroin-g-PNIPAM via Tyrosine-NIPAM Bridges
Authors: Radu Ionut-Cristian , Biru Iuliana-Elena , Damian Celina-Maria , Ion Andreea-Cristina , Iovu Horia , Tanasa Eugenia , Zaharia Catalin , Galateanu Bianca
Published: November 2019

This paper reports the synthesis and complex characterization of novel polymeric networks based on the crosslinking of Bombyx mori silk fibroin via poly(N-isopropylacrylamide) bridges generated by an ammonium cerium nitrate redox system. The research study gives an understanding of the polymerization mechanism in terms of the generation of radical sites, radical growth and termination reaction, as well as the involvement of modifications on silk fibroin structure and properties. The physico-chemical characterization was carried out by FTIR-ATR, X-ray photoelectron spectroscopy and RAMAN spectroscopy with unravelling the chemical modification. The structural characterization and spatial arrangement by secondary structure were carried out by X-ray diffraction and circular dichroism. The thermal behavior and thermal stability were evaluated by differential scanning calorimetry and thermogravimetric analysis. The novel complex polymer network is intended to be used in the field of smart drug delivery systems.
The Importance of Lactose in the Human Diet: Outcomes of a Mexican Consensus Meeting
Authors: Romero-Velarde Enrique , Delgado-Franco Dagoberto , García-Gutiérrez Mariana , Gurrola-Díaz Carmen , Larrosa-Haro Alfredo , Montijo-Barrios Ericka , Muskiet Frits A. J. , Vargas-Guerrero Belinda , Geurts Jan
Published: November 2019

Lactose is a unique component of breast milk, many infant formulas and dairy products, and is widely used in pharmaceutical products. In spite of that, its role in human nutrition or lactose intolerance is generally not well-understood. For that reason, a 2-day-long lactose consensus meeting with health care professionals was organized in Mexico to come to a set of statements for which consensus could be gathered. Topics ranging from lactase expression to potential health benefits of lactose were introduced by experts, and that was followed by a discussion on concept statements. Interestingly, lactose does not seem to induce a neurological reward response when consumed. Although lactose digestion is optimal, it supplies galactose for liver glycogen synthesis. In infants, it cannot be ignored that lactose-derived galactose is needed for the synthesis of glycosylated macromolecules. At least beyond infancy, the low glycemic index of lactose might be metabolically beneficial. When lactase expression decreases, lactose maldigestion may lead to lactose intolerance symptoms. In infancy, the temporary replacing of lactose by other carbohydrates is only justified in case of severe intolerance symptoms. In those who show an (epi)genetic decrease or absence of lactase expression, a certain amount (for adults mostly up to 12 g per portion) of lactose can still be consumed. In these cases, lactose shows beneficial intestinal-microbiota-shaping effects. Avoiding lactose-containing products may imply a lower intake of other important nutrients, such as calcium and vitamin B12 from dairy products, as well as an increased intake of less beneficial carbohydrates.
Proposal for an Empirical Japanese Diet Score and the Japanese Diet Pyramid
Authors: Kanauchi Masao , Kanauchi Kimiko
Published: November 2019

A traditional Japanese diet (JD) has been widely regarded as healthy, contributing to longevity. The modern Japanese lifestyle has become markedly westernized, and it is speculated that the number of people who eat JD is decreasing. A simple evaluation of people with low adherence to JD will help improve dietary life. We developed a simple assessment tool that can capture JD, and examined factors associated with low adherence to JD. A total of 1458 subjects aged 18 to 84 years completed a brief self-administered diet history questionnaire. We constructed an empirical Japanese diet score (eJDS) consisting of 12 items from the common characteristics of a JD. In our participants, 47.7% of subjects reported low adherence to JD and only 11.1% demonstrated high adherence. In multivariate logistic regression analysis, younger age persons, physically inactive persons, and heavy drinkers were associated with low adherence to JD. Based on the cutoff values of eJDS, we proposed to create a Japanese diet pyramid that is easy to use visually. In conclusion, the eJDS and the Japanese diet pyramid will be useful tools for nutrition education and dietary guidance.
Spanish Consumer Purchase Behaviour and Stated Preferences for Yoghurts with Nutritional and Health Claims
Authors: Ballco Petjon , De Magistris Tiziana
Published: November 2019

Nutritional and health claims are a useful tool for promoting healthier food choices and prevent non-communicable disease[s] (NCDs). Exhaustive literature that has investigated consumer evaluation of the presence of nutritional and/or health claim(s) during the decision-making process suggests that consumers’ sensitivity towards nutritional claims (NCs) and health claims (HCs) are still fragmented and should be further investigated. Our objective is to study the relationship between choice behaviour, attitudes and socio-demographic characteristics in order to evaluate the effectiveness of consumer characteristics in predicting Spanish consumers’ choice of products with NCs and HCs. A discrete choice experiment for yoghurt was conducted on a sample of 218 Spanish consumers, stratified by age, gender, education level, and income. Applying a latent class approach has enabled us to identify a niche of individuals, sensitive of NCs and HCs and to characterize them with respect to the rest of population. Results suggest that consumers positively valued most claims, however, the valuation was heterogeneous, and three consumer segments were identified: ‘health-claims oriented’, ‘nutritional- and health-claim oriented’ and ‘indifferent’. The results supply insights for the development of more targeted promotion campaigns, as well as for further actions in food marketing.
Bone Mineral Metabolism Status, Quality of Life, and Muscle Strength in Older People
Authors: Verde Zoraida , Giaquinta Andrea , Moreno Sainz Carmelo , Díaz Ondina Marta , Fernández Araque Ana
Published: November 2019

As the relationship between vitamin D and various diseases or health conditions has become known, interest in the contribution of vitamin D to overall health-related quality of life (QoL) has increased. We examined the relationship between vitamin D status and QoL in 273 participants aged 65 years and older. Serum levels of total calcium, phosphorus, intact parathyroid hormone, albumin, and 25-hydroxyvitaminD3 were analyzed. We also recruited data for QoL, physical activity, nutritional impairment, and muscular strength. Ninety percent of the subjects were classified as vitamin D deficient or insufficient. Participants with higher serum 25(OH)D3, calcium, phosphorous, and Alb levels were significantly less likely to self-report depression or anxiety after adjustment (p = 0.009, p = 0.005, p = 0.003, and p = 0.005, respectively). Additionally, we found an association between lower levels of albumin and self-reported problems with mobility or usual activities (p = 0.01). We also found associations between better muscle strength and higher levels of vitamin D, calcium, phosphorous, and albumin (p = 0.006, p = 0.003, p = 0.004 and p = 0.002, respectively). Overall, our data provide evidence that serum vitamin D and Alb levels are negatively related to self-reported anxiety or depression, usual activities, mobility, and three dimensions of QoL in older adults. Furthermore, vitamin D levels are positively related to hand grip strength in adults over 65 years old.
Associations of Less Healthy Snack Food Consumption with Infant Weight-for-Length Z-Score Trajectories: Findings from the Nurture Cohort Study
Authors: Moore Amy M. , Vadiveloo Maya , Tovar Alison , McCurdy Karen , Østbye Truls , Benjamin-Neelon Sara E.
Published: November 2019

Little is known about the impact of less healthy snack foods on weight trajectories during infancy. This secondary analysis of data from the Nurture cohort explored prospective associations of less healthy snack foods with infant weight trajectories. Pregnant women were recruited and, upon delivery of a single live infant, 666 mothers agreed to participate. Mothers completed sociodemographic and infant feeding questionnaires, and infant anthropometrics were collected during home visits at 3, 6, 9, and 12 months. Less healthy snack food consumption was assessed by asking how frequently baby snacks and sweets were consumed each day during the previous three months. Multilevel growth curve models explored associations of baby snacks and sweets with infant weight-for-length (WFL) z-scores. On average, mothers were 27 years old, 71.5% were non-Hispanic Black, and 55.4% had household incomes of ≤$20,000/year. Consumption of less healthy snack foods increased during infancy with a median intake of 3.0 baby snacks/day and 0.7 sweets/day between 10 and 12 months. Growth curve models showed that infants who consumed sweets >2 times/day had significantly higher WFL z-scores during the second half of infancy compared to infants who never consumed sweets. Less healthy snacks may contribute to the risk of obesity during infancy and promoting healthy snack food choices during this critical time is important.
New Zealand Bitter Hops Extract Reduces Hunger During a 24 h Water Only Fast
Authors: Walker Edward , Lo Kim , Tham Sze , Pahl Malcolm , Lomiwes Dominic , Cooney Janine , Wohlers Mark , Gopal Pramod
Published: November 2019

Intermittent fasting improves metabolic and cardiac health. However, increased hunger towards the end of the fasting period may affect compliance and limit its application. Our aim was to determine the effect of anorexigenic agent co-therapy on subjective ratings of appetite during the 16–24 h period of a day-long water-only intermittent fast. Thirty adult men were recruited and required to fast for 24 h from 18:00 h to 18:00 h on the same day of the week for three subsequent weeks. Treatments of either a placebo or one of two doses (high dose; HD: 250 mg or low dose; LD: 100 mg) of a bitter hops-based appetite suppressant (Amarasate®) were given twice per day at 16 and 20 h into the fast. From 18–24 h of the 24 h fast, both the HD and LD treatment groups exhibited a statistically significant (p < 0.05) > 10% reduction in hunger. Additionally, the expected lunchtime increase in hunger that was present in the placebo group (12:00 h) was absent in both the HD and LD groups. These data suggest that appetite suppressant co-therapy may be useful in reducing hunger during intermittent fasting, and show that bitter compounds may regulate appetite independently of meal timing.
A Combination of Aqueous Extraction and Polymeric Membranes as a Sustainable Process for the Recovery of Polyphenols from Olive Mill Solid Wastes
Authors: Conidi Carmela , Egea-Corbacho Agata , Cassano Alfredo
Published: November 2019

Polyamide commercial membranes in flat-sheet configuration and with molecular weight cut-off (MWCO) in the range of ultrafiltration (UF) to nanofiltration (NF) were tested for the recovery of phenolic compounds from clarified olive mill solid waste (OMSW) aqueous extracts. The performance of selected membranes was evaluated in terms of productivity (permeate flux) and selectivity towards biologically active compounds (such as phenolic compounds, flavanols, and hydroxycinnamic acids derivatives) and total antioxidant activity (TAA) as a function of transmembrane pressure (TMP). NF membranes produced higher permeate fluxes and a lower fouling index in comparison with UF membranes. Retention of bioactive compounds was also significantly higher for NF membranes than for UF membranes. In particular, membranes with MWCO in the range 150–500 Da showed rejection towards flavanols and hydroxycinnamic acid derivatives of about 100%. On the other hand, the rejection towards TAA and total polyphenols was of about 90% and 72%, respectively. Therefore, NF retentate fractions appear of practical interest for the production of food additives and food supplements due to their high antioxidant activity.
Sharing Nonfungible Information Requires Shared Nonfungible Information
Authors: Fields Chris , Marcianò Antonino
Published: November 2019

We show that sharing a quantum reference frame requires sharing measurement operators that identify the reference frame in addition to operators that measure its state. Observers restricted to finite resources cannot, in general, operationally determine that they share such operators. Uncertainty about whether system-identification operators are shared induces decoherence.
Comparison of Bi-Hemispherical and Hemispherical-Conical Configurations for In Situ Measurements of Solar-Induced Chlorophyll Fluorescence
Authors: Zhang Qian , Zhang Xiaokang , Li Zhaohui , Wu Yunfei , Zhang Yongguang
Published: November 2019

During recent decades, solar-induced chlorophyll fluorescence (SIF) has shown to be a good proxy for gross primary production (GPP), promoting the development of ground-based SIF observation systems and supporting a greater understanding of the relationship between SIF and GPP. However, it is unclear whether such SIF-oriented observation systems built from different materials and of different configurations are able to acquire consistent SIF signals from the same target. In this study, we used four different observation systems to measure the same targets together in order to investigate whether SIF from different systems is comparable. Integration time (IT), reflectance, and SIF retrieved from different systems with hemispherical-conical (hemi-con) and bi-hemispherical (bi-hemi) configurations were also evaluated. A newly built prism system (SIFprism, using prism to collect both solar and target radiation) has the shortest IT and highest signal to noise ratio (SNR). Reflectance collected from the different systems showed small differences, and the diurnal patterns of both red and far-red SIF derived from different systems showed a marginal difference when measuring the homogeneous vegetation canopy (grassland). However, when the target is heterogeneous, e.g., the Epipremnum aureum canopy, the values and diurnal pattern of far-red SIF derived from systems with a bi-hemi configuration were obviously different with those derived from the system with hemi-con configuration. These results demonstrate that different SIF systems are able to acquire consistent SIF for landscapes with a homogeneous canopy. However, SIF retrieved from bi-hemi and hemi-con configurations may be distinctive when the target is a heterogeneous (or discontinuous) canopy due to the different fields of view and viewing geometries. Our findings suggest that the bi-hemi configuration has an advantage to measure heterogeneous canopies due to the large field of view for upwelling sensors being representative for the footprint of the eddy covariance flux measurements.
RealPoint3D: Generating 3D Point Clouds from a Single Image of Complex Scenarios
Authors: Xia Yan , Wang Cheng , Xu Yusheng , Zang Yu , Liu Weiquan , Li Jonathan , Stilla Uwe
Published: November 2019

Generating 3D point clouds from a single image has attracted full attention from researchers in the field of multimedia, remote sensing and computer vision. With the recent proliferation of deep learning, various deep models have been proposed for the 3D point cloud generation. However, they require objects to be captured with absolutely clean backgrounds and fixed viewpoints, which highly limits their application in the real environment. To guide 3D point cloud generation, we propose a novel network, RealPoint3D, to integrate prior 3D shape knowledge into the network. Taking additional 3D information, RealPoint3D can handle 3D object generation from a single real image captured from any viewpoint and complex background. Specifically, provided a query image, we retrieve the nearest shape model from a pre-prepared 3D model database. Then, the image, together with the retrieved shape model, is fed into RealPoint3D to generate a fine-grained 3D point cloud. We evaluated the proposed RealPoint3D on the ShapeNet dataset and ObjectNet3D dataset for the 3D point cloud generation. Experimental results and comparisons with state-of-the-art methods demonstrate that our framework achieves superior performance. Furthermore, our proposed framework works well for real images in complex backgrounds (the image has the remaining objects in addition to the reconstructed object, and the reconstructed object may be occluded or truncated) with various viewing angles.
Decomposing the Long-term Variation in Population Exposure to Outdoor PM2.5 in the Greater Bay Area of China Using Satellite Observations
Authors: Lin Changqing , Lau Alexis K. H. , Fung Jimmy C. H. , He Qianshan , Ma Jun , Lu Xingcheng , Li Zhiyuan , Li Chengcai , Zuo Renguang , Wong Andromeda H. S.
Published: November 2019

The Greater Bay Area (GBA) of China is experiencing a high level of exposure to outdoor PM2.5 pollution. The variations in the exposure level are determined by spatiotemporal variations in the PM2.5 concentration and population. To better guide public policies that aim to reduce the population exposure level, it is essential to explicitly decompose and assess the impacts of different factors. This study took advantage of high-resolution satellite observations to characterize the long-term variations in population exposure to outdoor PM2.5 for cities in the GBA region during the three most-recent Five-Year Plan (FYP) periods (2001–2015). A new decomposition method was then used to assess the impact of PM2.5 variations and demographic changes on the exposure variation. Within the decomposition framework, an index of pollution-population-coincidence–induced PM2.5 exposure (PPCE) was introduced to characterize the interaction of PM2.5 and the population distribution. The results showed that the 15-year average PPCE levels in all cities were positive (e.g., 6 µg/m3 in Guangzhou), suggesting that unfavorable city planning had led to people dwelling in polluted areas. An analyses of the spatial differences in PM2.5 changes showed that urban areas experienced a greater decrease in PM2.5 concentration than did rural areas in most cities during the 11th (2006–2010) and 12th (2011–2015) FYP periods. These spatial differences in PM2.5 changes reduced the PPCE levels of these cities and thus reduced the exposure levels (by as much as -0.58 µg/m3/year). The population migration resulting from rapid urbanization, however, increased the PPCE and exposure levels (by as much as 0.18 µg/m3/year) in most cities during the three FYP periods considered. Dongguan was a special case in that the demographic change reduced the exposure level because of its rapid development of residential areas in cleaner regions adjacent to Shenzhen. The exposure levels in all cities remained high because of the high mean PM2.5 concentrations and their positive PPCE. To better protect public health, control efforts should target densely populated areas and city planning should locate more people in cleaner areas.
Winter Wheat Mapping Based on Sentinel-2 Data in Heterogeneous Planting Conditions
Authors: Zhang Dongyan , Fang Shengmei , She Bao , Zhang Huihui , Jin Ning , Xia Haoming , Yang Yuying , Ding Yang
Published: November 2019

Monitoring and mapping the spatial distribution of winter wheat accurately is important for crop management, damage assessment and yield prediction. In this study, northern and central Anhui province were selected as study areas, and Sentinel-2 imagery was employed to map winter wheat distribution and the results were verified with Planet imagery in the 2017–2018 growing season. The Sentinel-2 imagery at the heading stage was identified as the optimum period for winter wheat area extraction after analyzing the images from different growth stages using the Jeffries–Matusita distance method. Therefore, ten spectral bands, seven vegetation indices (VI), water index and building index generated from the image at the heading stage were used to classify winter wheat areas by a random forest (RF) algorithm. The result showed that the accuracy was from 93% to 97%, with a Kappa above 0.82 and a percentage error lower than 5% in northern Anhui, and an accuracy of about 80% with Kappa ranging from 0.70 to 0.78 and a percentage error of about 20% in central Anhui. Northern Anhui has a large planting scale of winter wheat and flat terrain while central Anhui grows relatively small winter wheat areas and a high degree of surface fragmentation, which makes the extraction effect in central Anhui inferior to that in northern Anhui. Further, an optimum subset data was obtained from VIs, water index, building index and spectral bands using an RF algorithm. The result of using the optimum subset data showed a high accuracy of classification with a great advantage in data volume and processing time. This study provides a perspective for winter wheat mapping under various climatic and complicated land surface conditions and is of great significance for crop monitoring and agricultural decision-making.
Validation of a Single RGB-D Camera for Gait Assessment of Polyneuropathy Patients
Authors: Vilas-Boas Maria do Carmo , Rocha Ana Patrícia , Choupina Hugo Miguel Pereira , Cardoso Márcio Neves , Fernandes José Maria , Coelho Teresa , Cunha João Paulo Silva
Published: November 2019

Motion analysis systems based on a single markerless RGB-D camera are more suitable for clinical practice than multi-camera marker-based reference systems. Nevertheless, the validity of RGB-D cameras for motor function assessment in some diseases affecting gait, such as Transthyretin Familial Amyloid Polyneuropathy (TTR-FAP), is yet to be investigated. In this study, the agreement between the Kinect v2 and a reference system for obtaining spatiotemporal and kinematic gait parameters was evaluated in the context of TTR-FAP. 3-D body joint data provided by both systems were acquired from ten TTR-FAP symptomatic patients, while performing ten gait trials. For each gait cycle, we computed several spatiotemporal and kinematic gait parameters. We then determined, for each parameter, the Bland Altman’s bias and 95% limits of agreement, as well as the Pearson’s and concordance correlation coefficients, between systems. The obtained results show that an affordable, portable and non-invasive system based on an RGB-D camera can accurately obtain most of the studied gait parameters (excellent or good agreement for eleven spatiotemporal and one kinematic). This system can bring more objectivity to motor function assessment of polyneuropathy patients, potentially contributing to an improvement of TTR-FAP treatment and understanding, with great benefits to the patients’ quality of life.
A Convolutional Neural Network for Impact Detection and Characterization of Complex Composite Structures
Authors: Tabian Iuliana , Fu Hailing , Sharif Khodaei Zahra
Published: November 2019

This paper reports on a novel metamodel for impact detection, localization and characterization of complex composite structures based on Convolutional Neural Networks (CNN) and passive sensing. Methods to generate appropriate input datasets and network architectures for impact localization and characterization were proposed, investigated and optimized. The ultrasonic waves generated by external impact events and recorded by piezoelectric sensors are transferred to 2D images which are used for impact detection and characterization. The accuracy of the detection was tested on a composite fuselage panel which was shown to be over 94%. In addition, the scalability of this metamodelling technique has been investigated by training the CNN metamodels with the data from part of the stiffened panel and testing the performance on other sections with similar geometry. Impacts were detected with an accuracy of over 95%. Impact energy levels were also successfully categorized while trained at coupon level and applied to sub-components with greater complexity. These results validated the applicability of the proposed CNN-based metamodel to real-life application such as composite aircraft parts.