bluuebunny/crossref_metadata_2025 huggingface dataset:
ALCAT1-Mediated Pathological Cardiolipin Remodeling and PLSCR3-Mediated Cardiolipin Transferring Contribute to LPS-Induced Myocardial Injury
Authors: Han Dong , Wang Chenyang , Feng Xiaojing , Hu Li , Wang Beibei , Hu Xinyue , Wu Jing
Published: September 2024

Cardiolipin (CL), a critical phospholipid situated within the mitochondrial membrane, plays a significant role in modulating intramitochondrial processes, especially in the context of certain cardiac pathologies; however, the exact effects of alterations in cardiolipin on septic cardiomyopathy (SCM) are still debated and the underlying mechanisms remain incompletely understood. This study highlights a notable increase in the expressions of ALCAT1 and PLSCR3 during the advanced stage of lipopolysaccharide (LPS)-induced SCM. This up-regulation potential contribution to mitochondrial dysfunction and cellular apoptosis—as indicated by the augmented oxidative stress and cytochrome c (Cytc) release—coupled with reduced mitophagy, decreased levels of the antiapoptotic protein B-cell lymphoma-2 (Bcl-2) and lowered cell viability. Additionally, the timing of LPS-induced apoptosis coincides with the decline in both autophagy and mitophagy at the late stages, implying that these processes may serve as protective factors against LPS-induced SCM in HL-1 cells. Together, these findings reveal the mechanism of LPS-induced CL changes in the center of SCM, with a particular emphasis on the importance of pathological remodeling and translocation of CL to mitochondrial function and apoptosis. Additionally, it highlights the protective effect of mitophagy in the early stage of SCM. This study complements previous research on the mechanism of CL changes in mediating SCM. These findings enhance our understanding of the role of CL in cardiac pathology and provide a new direction for future research.
Transcranial Magnetic Stimulation Facilitates Neural Speech Decoding
Authors: Comstock Lindy , Carvalho Vinícius Rezende , Lainscsek Claudia , Fallah Aria , Sejnowski Terrence J.
Published: September 2024

Transcranial magnetic stimulation (TMS) has been widely used to study the mechanisms that underlie motor output. Yet, the extent to which TMS acts upon the cortical neurons implicated in volitional motor commands and the focal limitations of TMS remain subject to debate. Previous research links TMS to improved subject performance in behavioral tasks, including a bias in phoneme discrimination. Our study replicates this result, which implies a causal relationship between electro-magnetic stimulation and psychomotor activity, and tests whether TMS-facilitated psychomotor activity recorded via electroencephalography (EEG) may thus serve as a superior input for neural decoding. First, we illustrate that site-specific TMS elicits a double dissociation in discrimination ability for two phoneme categories. Next, we perform a classification analysis on the EEG signals recorded during TMS and find a dissociation between the stimulation site and decoding accuracy that parallels the behavioral results. We observe weak to moderate evidence for the alternative hypothesis in a Bayesian analysis of group means, with more robust results upon stimulation to a brain region governing multiple phoneme features. Overall, task accuracy was a significant predictor of decoding accuracy for phoneme categories (F(1,135) = 11.51, p < 0.0009) and individual phonemes (F(1,119) = 13.56, p < 0.0003), providing new evidence for a causal link between TMS, neural function, and behavior.
Early Auditory Temporal Processing Deficit in Children with Autism Spectrum Disorder: The Research Domain Criteria Framework
Authors: Sanglakh Ghoochan Atigh Atoosa , Joghataei Mohammad Taghi , Moradkhani Shadi , Alizadeh Zarei Mehdi , Nazari Mohammad Ali
Published: September 2024

Altered sensory processing especially in the auditory system is considered a typical observation in children with autism spectrum disorder (ASD). Auditory temporal processing is known to be impaired in ASD children. Although research suggests that auditory temporal processing abnormalities could be responsible for the core aspects of ASD, few studies have examined early time processing and their results have been conflicting. The present event-related potential (ERP) study investigated the early neural responses to duration and inter-stimulus interval (ISI) deviants in nonspeech contexts in children with ASD and a control group of typically developing (TD) children matched in terms of age and IQ. A passive auditory oddball paradigm was employed to elicit the mismatch negativity (MMN) for change detection considering both the duration and ISI-based stimulus. The MMN results showed that the ASD group had a relatively diminished amplitude and significant delayed latency in response to duration deviants. The findings are finally discussed in terms of hyper-hyposensitivity of auditory processing and the fact that the observed patterns may potentially act as risk factors for ASD development within the research domain criteria (RDoC) framework.
A National Profile of Latino Serial Entrepreneurs in the United States of America
Authors: Pisani Michael J.
Published: September 2024

This study examines the scope and determinants of serial entrepreneurship among employer firms of Latino-owned businesses (LOBs) in the USA. The primary data are derived from the 2023 Stanford Latino Entrepreneurship Initiative nationally representative survey of 5102 LOBs and Latino entrepreneurs. The scope of Latino serial entrepreneurship—entrepreneurs who have started more than one business—reveals approximately 30% of Latino entrepreneurs have started two or more enterprises. The determinants of serial entrepreneurship are derived from entrepreneur demographics, utilizing binomial logistic regression to predict the odds of Latino serial entrepreneurship. Findings suggest Latino serial entrepreneurs are more likely to be male, native US-born, identify less as Latino, reside in the Midwest and South, possess high levels of education, and are of Mexican descent.
LMNA-Related Dilated Cardiomyopathy: Single-Cell Transcriptomics during Patient-Derived iPSC Differentiation Support Cell Type and Lineage-Specific Dysregulation of Gene Expression and Development for Cardiomyocytes and Epicardium-Derived Cells with Lamin A/C Haploinsufficiency
Authors: Zaragoza Michael V. , Bui Thuy-Anh , Widyastuti Halida P. , Mehrabi Mehrsa , Cang Zixuan , Sha Yutong , Grosberg Anna , Nie Qing
Published: September 2024

LMNA-related dilated cardiomyopathy (DCM) is an autosomal-dominant genetic condition with cardiomyocyte and conduction system dysfunction often resulting in heart failure or sudden death. The condition is caused by mutation in the Lamin A/C (LMNA) gene encoding Type-A nuclear lamin proteins involved in nuclear integrity, epigenetic regulation of gene expression, and differentiation. The molecular mechanisms of the disease are not completely understood, and there are no definitive treatments to reverse progression or prevent mortality. We investigated possible mechanisms of LMNA-related DCM using induced pluripotent stem cells derived from a family with a heterozygous LMNA c.357-2A>G splice-site mutation. We differentiated one LMNA-mutant iPSC line derived from an affected female (Patient) and two non-mutant iPSC lines derived from her unaffected sister (Control) and conducted single-cell RNA sequencing for 12 samples (four from Patients and eight from Controls) across seven time points: Day 0, 2, 4, 9, 16, 19, and 30. Our bioinformatics workflow identified 125,554 cells in raw data and 110,521 (88%) high-quality cells in sequentially processed data. Unsupervised clustering, cell annotation, and trajectory inference found complex heterogeneity: ten main cell types; many possible subtypes; and lineage bifurcation for cardiac progenitors to cardiomyocytes (CMs) and epicardium-derived cells (EPDCs). Data integration and comparative analyses of Patient and Control cells found cell type and lineage-specific differentially expressed genes (DEGs) with enrichment, supporting pathway dysregulation. Top DEGs and enriched pathways included 10 ZNF genes and RNA polymerase II transcription in pluripotent cells (PP); BMP4 and TGF Beta/BMP signaling, sarcomere gene subsets and cardiogenesis, CDH2 and EMT in CMs; LMNA and epigenetic regulation, as well as DDIT4 and mTORC1 signaling in EPDCs. Top DEGs also included XIST and other X-linked genes, six imprinted genes (SNRPN, PWAR6, NDN, PEG10, MEG3, MEG8), and enriched gene sets related to metabolism, proliferation, and homeostasis. We confirmed Lamin A/C haploinsufficiency by allelic expression and Western blot. Our complex Patient-derived iPSC model for Lamin A/C haploinsufficiency in PP, CM, and EPDC provided support for dysregulation of genes and pathways, many previously associated with Lamin A/C defects, such as epigenetic gene expression, signaling, and differentiation. Our findings support disruption of epigenomic developmental programs, as proposed in other LMNA disease models. We recognized other factors influencing epigenetics and differentiation; thus, our approach needs improvement to further investigate this mechanism in an iPSC-derived model.
Sleep Quality and Medication Adherence in Older Adults: A Systematic Review
Authors: Amato Leandro , Giannetta Noemi , Taborri Sofia , Dionisi Sara , Panattoni Nicolò , Di Simone Emanuele , De Leo Aurora , Liquori Gloria , Orsi Giovanni Battista , Fabbian Fabio , Di Muzio Marco
Published: September 2024

Sleep quality significantly impacts individuals’ overall health, especially in older adults. Older adults often face comorbid conditions, polypharmacy (the intake of five or more medications per day), and medication non-adherence, which are common among those with sleep disorders. The purpose of this systematic review is to examine the relationship between sleep quality and medication adherence in older adults and to describe the measuring instruments used. A systematic review was performed following the PRISMA guidelines. The PubMed, Scopus, Cochrane Library, and CINAHL databases were screened from January 2024 to March 2024. Nine articles were included in the final synthesis based on the inclusion and exclusion criteria. The review found that poor sleep quality is significantly associated with reduced medication adherence in older adults. The key sleep determinants linked to medication non-adherence include sleep disorders, sleep efficiency, sleep duration, and daytime dysfunctions. Inappropriate medication prescriptions have been associated with reduced sleep efficiency. The tools for assessing sleep quality and medication adherence are predominantly subjective and varied. To address these challenges, a comprehensive geriatric assessment should include investigations into sleep disorders and comorbidity factors. Additionally, nursing educational interventions could be pivotal in improving medication adherence among older adults.
An Assessment of Tropical Cyclone Frequency in the Bay of Bengal and Its Impact on Coastal Bangladesh
Authors: Wahiduzzaman Md , Yeasmin Alea
Published: September 2024

This study examines the frequency of tropical cyclones in the Bay of Bengal and their impact on Bangladesh. The extent of environmental harm led to the selection of two specific areas: the Panpatty Union and Galachipa Upzilla in the Patuakhali district, and the Sariakat Union and Swandip Upzilla in the Chittagong district. The results indicate that cyclonic storms are more common in May and November. The results also demonstrate that the studied regions are vulnerable to the effects of tropical cyclones and suffer significant consequences. The differences in influence between the two locations are statistically significant with a confidence level of 90%. The findings have significant ramifications for policymaking decisions.
Effect of High Temperature on Micro-Structure and Mechanical Properties of Fiber-Reinforced Cement-Based Composites
Authors: Ge Chen , Chen Xiaoliang , Gong Yongfan , Kong Xijun , Chen Fei
Published: September 2024

Synthetic fibers can effectively inhibit the formation and propagation of micro-cracks in concrete, significantly reducing the number and scale of cracks within the concrete matrix, thereby enhancing the concrete’s crack resistance and seepage prevention capabilities. In this study, two types of synthetic fibers, polyvinyl alcohol (PVA) and polypropylene (PP), were incorporated into cement mortar to investigate their microstructural evolution at elevated temperatures and their influence on the mechanical properties of the mortar. Both fibers were added at a volume content of 0.5%. The mortar samples were subjected to the following temperature conditions: 20 °C (ambient), 200 °C, 400 °C, and 500 °C. The results indicate that the synthetic fibers employed in this study improved the tensile properties of the mortar at room temperature (20 °C). This enhancement persisted up to 400 °C, beyond which, at 500 °C, the mechanical properties of the fiber-reinforced mortar deteriorated significantly. At 400 °C, the tensile strength of the PVA group increased by approximately 16% compared to the unblended fiber group (JZ) and by about 45% compared to the PP group. After treatment at 500 °C, the tensile strength of mortar specimens in the PVA group and the PP group decreased by 36.47% and 24.14%, respectively, compared with that at 20 °C. The porous structure formed due to the high-temperature ablation of the synthetic fibers contributed to relieving the internal pressure within the mortar.
Going Inside a Historical Brazilian Diamond from the Spada Collection (19th Century)
Authors: Agrosì Giovanna , Mele Daniela , Tempesta Gioacchino
Published: September 2024

The characterization of objects of historical and cultural interest represents a crucial topic, specifically when it regards gemstones. Actually, the advanced investigation of precious minerals of gemological interest requires exclusively non-destructive analyses which are also suitable for determining their provenance when it is unknown. In this study, a non-destructive analytical protocol, previously tested on diamonds for petrogenetic studies, has been applied to a natural diamond of very high historical and gemological value, donated in 1852 by Monsignor Lavinio de ‘Medici Spada to the Museum of Earth Sciences of Sapienza University (Rome). The analytical protocol used includes X-ray diffraction topography, micro-computed X-ray tomography, single-crystal micro-X-ray diffraction and Fourier-transform infrared spectroscopy. The results show the presence of dislocations originating from inclusions and a very low degree of plastic deformation. The aggregation states of its N impurities show that this diamond is type IaAB, while the inclusions consist of olivine (Fo92-93), suggesting a lithospheric origin. The historical references found in the catalogs of the Museum indicate only a Brazilian origin, without any reference to the mining district. The information acquired in this study, enhanced by document research on mining in Brazil since 1700, suggests that the diamond likely comes from the district of Diamantina, Mina Gerais, Brazil.
Environmental and Energy Applications of Graphene-Based Nanocomposites: A Brief Review
Authors: Prasad N. V. Krishna , Naidu K. Chandra Babu , Baba Basha D.
Published: September 2024

Chemically stable two-dimensional nanostructured graphene with huge surface area, high electrical conductivity and mechanical excellence has gained significant research attention in the past two decades. Its excellent characteristics make graphene one of the important materials in various applications such as environmental and energy storage devices. Graphene no doubt has been a top priority among the carbon nanomaterials owing to its structure and properties. However, the functionalization of graphene leads to various nanocomposites where its properties are tailored to be suited for various applications with more performance, environmental friendliness, efficiency, durability and cost effectiveness. Graphene nanocomposites are said to exhibit more surface area, conductivity, power conversion efficiency and other characteristics in energy devices like supercapacitors. This review was aimed to present some of the applications of graphene-based nanocomposites in energy conversion devices like supercapacitors and Li-ion batteries and some of the environmental applications. It was observed that the performance of supercapacitors was obstructed due to restacking and agglomeration of graphene layers. This was addressed by combining MO (metal oxide) or CP (conducting polymer) with graphene as material for electrodes. Electrodes with CP or MO/graphene composites are summarized. Heterogeneous catalysts were of environmental concern in recent years. In this context, graphene-based nanocomposites gained significance due to expansion in structural diversity. A minimum overview is presented in this paper in terms of structural aspects and properties of GO/rGO-based materials used in supercapacitors and environmental applications like dye removal. Continuous efforts towards synthesis of productive graphene-based nanocomposites might lead to significant output in applications related to environment and energy sectors.
Hydrometallurgical Technology for Producing Rhenium(VII) and Cobalt(II) from Waste
Authors: Leszczyńska-Sejda Katarzyna , Malarz Joanna , Ciszewski Mateusz , Kopyto Dorota , Goc Karolina , Grzybek Alicja , Kowalik Patrycja , Orda Szymon , Pianowska Karolina , Turczyńska Aleksandra , Benke Grzegorz
Published: September 2024

This paper presents a method for obtaining cobalt(II) perrhenate from waste derived from two types of materials, i.e., Li-ion battery scrap, or more precisely, battery mass, and superalloy scrap. Both of the above-mentioned materials are a source of Co. However, a source of rhenium is perrhenic acid produced from ammonium perrhenate (recycled) by the ion exchange method using resins. Co(OH)2 can be precipitated from solutions resulting from the leaching of Li-ion battery mass, sludge from the Zn-Pb industry and superalloy scrap. The compound, after proper purification, can be used in a reaction with perrhenic acid to form Co(ReO4)2. The reaction should be conducted under the following conditions: time 1 h, room temperature, 30% excess of cobalt(II) hydroxide, and rhenium concentration in HReO4 from about 20 g/dm3 to 300 g/dm3. This work shows that with the use of Co(OH)2, obtained from waste, an anhydrous form of cobalt(II) perrhenate can be obtained, containing < 1000 ppm of the cumulative metal impurities.
Proteinuric and Non-Proteinuric Diabetic Kidney Disease: Different Presentations of the Same Disease?
Authors: Fabre Larissa , Pedregosa-Miguel Juliana Figueredo , Rangel Érika Bevilaqua
Published: September 2024

Background: Diabetic kidney disease (DKD) is a leading cause of end-stage kidney disease (ESKD) worldwide. This review examines the potential differences in clinical presentation, outcomes, and management between individuals with proteinuric DKD (P-DKD) and non-proteinuric DKD (NP-DKD). Methods: We analyzed articles published globally from 2000 and 2024. Results: Individuals with NP-DKD generally have lower blood pressure levels and a more favorable lipid profile. In contrast, histological studies show that P-DKD is associated with more severe glomerulosclerosis, mesangial expansion, arteriolar hyalinosis, interstitial-fibrosis/tubular atrophy, and immune complex deposits. Additionally, those with P-DKD are more likely to develop diabetic retinopathy and have a higher risk of all-cause mortality and progression to ESKD. Strategies to slow DKD progression, applicable to both NP-DKD and P-DKD, include non-pharmacologic and pharmacologic interventions such as renin–angiotensin system blockers, sodium-glucose co-transporter-2 inhibitors, finerenone, and glucagon-like protein receptor agonists. Conclusions: NP-DKD and P-DKD represent different presentations of the same underlying disease.
Passive Islanding Detection of Inverter-Based Resources in a Noisy Environment
Authors: Amini Hossein , Mehrizi-Sani Ali , Noroozian Reza
Published: September 2024

Islanding occurs when a load is energized solely by local generators and can result in frequency and voltage instability, changes in current, and poor power quality. Poor power quality can interrupt industrial operations, damage sensitive electrical equipment, and induce outages upon the resynchronization of the island with the grid. This study proposes an islanding detection method employing a Duffing oscillator to analyze voltage fluctuations at the point of common coupling (PCC) under a high-noise environment. Unlike existing methods, which overlook the noise effect, this paper mitigates noise impact on islanding detection. Power system noise in PCC measurements arises from switching transients, harmonics, grounding issues, voltage sags and swells, electromagnetic interference, and power quality issues that affect islanding detection. Transient events like lightning-induced traveling waves to the PCC can also introduce noise levels exceeding the voltage amplitude by more than seven times, thus disturbing conventional detection techniques. The noise interferes with measurements and increases the nondetection zone (NDZ), causing failed or delayed islanding detection. The Duffing oscillator nonlinear dynamics enable detection capabilities at a high noise level. The proposed method is designed to detect the PCC voltage fluctuations based on the IEEE standard 1547 through the Duffing oscillator. For the voltages beyond the threshold, the Duffing oscillator phase trajectory changes from periodic to chaotic mode and sends an islanded operation command to the inverter. The proposed islanding detection method distinguishes switching transients and faults from an islanded operation. Experimental validation of the method is conducted using a 3.6 kW PV setup.
Recent Development of Thermal Insulating Materials for Li-Ion Batteries
Authors: Quan Ting , Xia Qi , Wei Xiaoyu , Zhu Yanli
Published: September 2024

As one of the core components of electric vehicles, Li-ion batteries (LIBs) have attracted intensive attention due to their high energy density and good long-term cycling stability. However, some abuse conditions inevitably occur during battery operation, resulting in safety accidents such as the thermal runaway (TR) of LIBs. Therefore, the efficient and appropriate thermal insulation material design is crucial for LIB packs to effectively reduce or even inhibit the spread of TR. Based on it, in this review, we present the principle and influences of TR to provide the necessity of battery thermal management and thermal insulating materials. Then, we deeply discuss and compare the two kinds of representative thermal insulating materials: phase change thermal insulating materials and barrier-type thermal insulating materials. Their properties, synthesis methods, and modification means are investigated to provide some guidance for the future application of high-performance thermal insulating materials in the field of LIBs.
Process Optimization and Analysis of Product Quality of Blueberry and Corn Peptide Fermented by Mixed Lactic Acid Bacteria
Authors: Cong Shanzi , Zhang Xinxin , Zhao Hongji , Sun Meng , Hu Nan
Published: September 2024

To study the mixed fermentation technology of blueberry and corn peptide by Lacticaseibacillus rhamnosus ZYN-0417 and Lactiplantibacillus plantarum ZYN-0221 and its effect on fermentation quality, fermentation conditions were optimized using a single-factor test and a Plackett–Burman design combined with a Box–Behnken response surface methodology, with blueberry juice and corn peptide as raw materials and L. rhamnosus ZYN-0417 and Lp. plantarum ZYN-0221 as the starter. The results showed that the optimum fermentation conditions were as follows: the volume ratio of ZYN-0417 and ZYN-0221 was 1:1, the amount of blueberry juice was 20%, the amount of corn peptide was 22%, the amount of glucose was 2%, the amount of inoculation was 5%, the fermentation time was 40 h, and the fermentation temperature was 37 °C. Under optimum conditions, the viable count of lactic acid bacteria in fermented blueberry and corn peptide was 16.28 log CFU/mL, the pH value was 4.86, the total acid content was 14.00 g/L, the total sugar content was 39.98 g/100 g, the protein content was 48.34 g/100 g, the scavenging ability of the DPPH, ABTS+ and ·OH− radicals increased by 10.14%, 10.98% and 25.02%, respectively, the chelating ability of Fe2+ increased by 14.92%, the content of total phenol increased by 1.43 mg/L, the content of total flavonoids increased by 0.38 mg/L, the activity of superoxide dismutase increased by 122.10 U/mL, and the activation rate of alcohol dehydrogenase increased by 5.74%. The results showed that mixed lactic acid bacteria could be used as a starter of blueberry and corn peptide and effectively improve the nutritional value of the product.
Enhancement of Active Substances in Astragali Radix Broth with Lactic Acid Bacteria Fermentation and the Promotion Role of Chlorella Growth Factor
Authors: Li Xiaomeng , Liu Wei , Ge Qingyan , Xu Tongtong , Wu Xiang , Zhong Ruohui
Published: September 2024

Astragali Radix, a traditional Chinese herbal medicine widely used for its medicinal properties, is known to be rich in active components that possess various pharmacological effects. However, the effectiveness of microbial fermentation in enhancing the content of these active substances remains unclear. In this study, a microflora of lactic acid bacteria was used to ferment Astragali Radix, and the promoting effect of Chlorella Growth Factor (CGF) on the fermentation process was investigated so as to clarify the changes in major active compound content in the fermented Astragali Radix broth. Non-targeted metabolomic analysis based on ultra-high-performance liquid chromatography–mass spectrometry was conducted to analyze the differences in metabolites before and after fermentation. The results showed that the total polysaccharide, total flavonoid, and total saponin content in the fermented Astragali Radix broth increased by up to 51.42%, 97.76%, and 72.81% under the optimized conditions, respectively. Streptococcus lutetiensis was the dominant bacterial species during the fermentation process. There were significant differences in metabolites in the fermentation broth before and after fermentation, among which amino acids (such as L-Aspartyl-L-Phenylalanine, etc.) and saponin compounds (such as Cloversaponin I, Goyasaponin I, etc.) were the main upregulated metabolites, which can enhance the physiological functions of Astragali Radix fermentation broth. The CGF exhibited the ability to promote the increase of active substance content in the fermented Astragali Radix broth.
Workflow Trace Profiling and Execution Time Analysis in Quantitative Verification
Authors: Su Guoxin , Liu Li
Published: September 2024

Workflows orchestrate a collection of computing tasks to form a complex workflow logic. Different from the traditional monolithic workflow management systems, modern workflow systems often manifest high throughput, concurrency and scalability. As service-based systems, execution time monitoring is an important part of maintaining the performance for those systems. We developed a trace profiling approach that leverages quantitative verification (also known as probabilistic model checking) to analyse complex time metrics for workflow traces. The strength of probabilistic model checking lies in the ability of expressing various temporal properties for a stochastic system model and performing automated quantitative verification. We employ semi-Makrov chains (SMCs) as the formal model and consider the first passage times (FPT) measures in the SMCs. Our approach maintains simple mergeable data summaries of the workflow executions and computes the moment parameters for FPT efficiently. We describe an application of our approach to AWS Step Functions, a notable workflow web service. An empirical evaluation shows that our approach is efficient for computer high-order FPT moments for sizeable workflows in practice. It can compute up to the fourth moment for a large workflow model with 10,000 states within 70 s.
Proximate Analysis and Techno-Functional Properties of Berberis aristata Root Powder: Implications for Food Industry Applications
Authors: Awari Ankita , Kumar Mukul , Kaushik Deepika , Amarowicz Ryszard , Proestos Charalampos , Wahab Rizwan , Khan Mohammad Rizwan , Tomasevic Igor , Oz Emel , Oz Fatih
Published: September 2024

Berberis aristata, commonly known as Indian barberry, has been traditionally used for its medicinal properties. Despite its recognized pharmacological benefits, its potential application in the food industry remains underexplored. This study aims to investigate the proximate analysis and techno-functional properties of Berberis aristata root powder to evaluate its feasibility as a functional food ingredient. The root powder of Berberis aristata was subjected to proximate analysis to determine its moisture, ash, protein, fat, fiber, and carbohydrate content. Techno-functional properties, including water and oil absorption capacity, emulsifying and foaming properties, and bulk density, were evaluated using standardized analytical techniques. The proximate analysis revealed a high fiber content and a significant number of bioactive compounds. The root powder exhibited favorable water and oil absorption capacities, making it suitable for use as a thickening and stabilizing agent. Emulsifying and foaming properties were comparable to conventional food additives, indicating their potential in various food formulations. The findings suggest that Berberis aristata root powder possesses desirable techno-functional properties that could be leveraged in the food industry. Its high fiber content and bioactive compounds offer additional health benefits, making it a promising candidate for functional food applications. Further research on its incorporation into different food matrices and its sensory attributes is recommended to fully establish its utility.
Rice (Oryza sativa) Stem Cells as a Novel Promising Active Ingredient with Anti-Proliferative Effects for Potential Skin Cancer Prevention and Skin Whitening Activity
Authors: Asadollahi Leila , Abbaspour-Ravasjani Soheil , Kim Kyung Ah , Maghsoodi Maryam , Hamishehkar Hamed , Kosari-Nasab Morteza , Kim Ki Hyun
Published: September 2024

Rice is one of the plants proven to possess antioxidant, anti-inflammatory, anti-proliferative, and whitening properties, making it one of the most beneficial plants in this regard. This study aimed to introduce a novel natural cosmetic and pharmaceutical product based on rice callus as a source of active ingredients that can inhibit skin melanoma cell (B16F10) proliferation and brighten the skin. The 2,4-D hormone at concentrations of 1 µg/mL and 1.5 µg/mL was used to induce rice callus formation. Rice callus extracts were then prepared using aqueous and ethanolic solvents, with a concentration of 1 mg/mL used for characterization tests. To determine the optimal hormone concentration, the phenols/flavonoids, antioxidant activity, proteins, and carbohydrates in the extracts were measured. The optimal concentration of the hormone was found to be 1 µg/mL. Finally, the anti-melanocyte and skin-whitening activity of the extracts was assessed through measurements of their cytotoxicity and inhibition of melanin synthesis-related enzymes. Cellular cytotoxicity measurements revealed that the ethanolic extract induced more cytotoxicity than the aqueous extract, with IC50 values of 566.3 µg/mL for the ethanolic extract and 1327 µg/mL for the aqueous extract. Skin-whitening-related tests demonstrated that the extracts were 1.7 times more effective than arbutin in inhibiting factors that cause hyperpigmentation. The aqueous extract achieved 85% inhibition of melanin biosynthesis at a concentration of 3200 µg/mL, compared to 68% for the ethanolic extract and 50% for arbutin. Based on these findings, rice callus extract can be introduced as a new, effective substance for skin-lightening and anti-melanocyte products in cosmeceutical and pharmaceutical formulations.
Cell-Free DNA Hydroxymethylation in Cancer: Current and Emerging Detection Methods and Clinical Applications
Authors: Li Janice J. N. , Liu Geoffrey , Lok Benjamin H.
Published: September 2024

In the era of precision oncology, identifying abnormal genetic and epigenetic alterations has transformed the way cancer is diagnosed, managed, and treated. 5-hydroxymethylcytosine (5hmC) is an emerging epigenetic modification formed through the oxidation of 5-methylcytosine (5mC) by ten-eleven translocase (TET) enzymes. DNA hydroxymethylation exhibits tissue- and cancer-specific patterns and is essential in DNA demethylation and gene regulation. Recent advancements in 5hmC detection methods and the discovery of 5hmC in cell-free DNA (cfDNA) have highlighted the potential for cell-free 5hmC as a cancer biomarker. This review explores the current and emerging techniques and applications of DNA hydroxymethylation in cancer, particularly in the context of cfDNA.
Simultaneous Visualization of R-Loops/RNA:DNA Hybrids and Replication Forks in a DNA Combing Assay
Authors: Ivanov Miroslav Penchev , Zecchini Heather , Hamerlik Petra
Published: September 2024

R-loops, structures that play a crucial role in various biological processes, are integral to gene expression, the maintenance of genome stability, and the formation of epigenomic signatures. When these R-loops are deregulated, they can contribute to the development of serious health conditions, including cancer and neurodegenerative diseases. The detection of R-loops is a complex process that involves several approaches. These include S9.6 antibody- or RNAse H-based immunoprecipitation, non-denaturing bisulfite footprinting, gel electrophoresis, and electron microscopy. Each of these methods offers unique insights into the nature and behavior of R-loops. In our study, we introduce a novel protocol that has been developed based on a single-molecule DNA combing assay. This innovative approach allows for the direct and simultaneous visualization of RNA:DNA hybrids and replication forks, providing a more comprehensive understanding of these structures. Our findings confirm the transcriptional origin of the hybrids, adding to the body of knowledge about their formation. Furthermore, we demonstrate that these hybrids have an inhibitory effect on the progression of replication forks, highlighting their potential impact on DNA replication and cellular function.
Assessing Community Resilience to Flood Disasters: A Case Study of Temerloh, Pahang
Authors: Rameli Nor Lita Fadilah , Majid Nuriah Abd , Mohamed Ahmad Fariz
Published: September 2024

Rapid urbanization and development have led to a significant number of Malaysians living in flood-prone areas, exacerbating the impact of natural disasters such as floods. This study aims to identify the factors influencing community resilience both before and after flood events in Temerloh, Pahang. Data were collected via questionnaires distributed to 350 flood victims. Key findings reveal that long-term residents exhibit higher resilience due to local knowledge and preparedness strategies. The study highlights the importance of integrating local experiences into flood management policies to enhance community resilience. The results reveal that large flood-prone areas experience frequent flooding, contributing to considerable community distress. This distress is somewhat alleviated by the support provided by government agencies and NGOs, which includes relief efforts, emergency services, and flood defense construction. Additionally, community involvement in flood management was found to have a statistically significant impact (p < 0.05). The study highlights the need for enhanced flood management strategies that address psychological aspects to reduce negative impacts on the community.
Anxiety and Depression after Spinal Cord Injury: A Cross-Sectional Study
Authors: Molina-Gallego Brigida , Ugarte-Gurrutxaga María Idoia , Molina-Gallego Laura , Plaza del Pino Fernando Jesús , Carmona-Torres Juan Manuel , Santacruz-Salas Esmeralda
Published: September 2024

Spinal cord injury (SCI) is a life-changing event that often results in chronic physical damage and challenges in maintaining a good quality of life as it affects every aspect of life. These situations require adjustment, increasing vulnerability to psychological disorders. The objective of this study was to evaluate the impact of SCI on psychological morbidity in individuals with subacute and chronic SCI. The present investigation was designed to determine the presence and extent of psychological complications following SCI. We used two reliable questionnaires and validated psychological assessments to study depression (BDI) and anxiety (STAI), a broad range of factors derived from SCI that may be predictors of certain psychological problems. The psychological assessment revealed alterations in depression and anxiety, although the data do not exceed those of previous investigations. No clear predisposing factors leading to certain psychological pathologies were found. In addition, individuals in the subacute and chronic stages differed in their scores. In individuals with SCI, identifying predictors of psychological problems is difficult, but premature assessment of mental state is essential. This early diagnosis of possible problems or changes at the mental level is fundamental and necessary to avoid possible alterations at the cognitive level and, of course, more serious mental complications.
Syndromic Surveillance in Public Health Emergencies: A Systematic Analysis of Cases Related to Exposure to 2023 Floodwaters in Romagna, Italy
Authors: Montalti Marco , Fabbri Marco , Angelini Raffaella , Bakken Elizabeth , Morri Michela , Tamarri Federica , Reali Chiara , Soldà Giorgia , Silvestrini Giulia , Lenzi Jacopo
Published: September 2024

Background: In May 2023, Romagna, Italy, faced a devastating flood resulting in 16 fatalities, forced displacement of 26,000 citizens, and significant economic losses. Due to potential water contamination, implementing public health strategies became imperative for the Local Health Authority to mitigate the health consequences, analyze the flood’s impact on the local population’s health, and detect early anomalies requiring timely public health interventions. Methods: Between June and July 2023, general practitioners who were part of the RespiVirNet surveillance network completed weekly structured forms. These forms collected data on individuals exposed or not to floodwaters and clinical syndromes. Rates per 1000 resident population aged > 14 were stratified by district, week of observation, and symptomatology. Missing data were addressed by imputation using second-order autoregressive modeling. Results: An incidence of 3.52 syndromes potentially related to flood water exposure per 1000 individuals (95% CI 2.82–4.35) was estimated. Ravenna, the city most affected by the flood, recorded the highest rate (6.05 per 1000, 95% CI 4.59–7.82). Incidence decreased in the weeks post-event. Anxiety, or trauma and stress symptoms, exhibited higher rates among the exposed, diminishing over weeks. The incidence for the non-exposed (12.76 per 1000, 95% CI 10.55–15.29) showed no significant territorial differences compared to the exposed ones. Conclusions: Syndromic surveillance provided timely information on the flood’s health impact, revealing a higher incidence of individual syndromes among the non-exposed. This study contributes to guiding the implementation of future public health preparedness and response strategies for populations facing similar natural disasters.
Navigating Maritime Heritage: An Immersive Virtual Tour of the USS Drum Submarine Museum
Authors: Liu Junshan , Willkens Danielle S. , Kim Jeffery Scott
Published: September 2024

Virtual Reality (VR) technology has revolutionized the preservation and interpretation of heritage sites. This study focuses on developing an immersive 360-degree virtual tour (VT) for the USS Drum Submarine Museum in Mobile, Alabama, USA, incorporating oral histories to enhance the accessibility and visitor experiences. The project addresses the need for innovative methods to present maritime history effectively. Using Matterport technology, detailed 3D imagery of the USS Drum was captured and processed, integrating multimedia elements and oral histories from a veteran USS Drum crew member to provide a richer historical narrative. A user experience study gathered feedback from virtual visitors, who offered quantitative and qualitative responses. The research findings indicate that the VT significantly enhances visitor engagement and historical understanding, with high satisfaction rates for visual quality and oral histories, though some users experienced technical challenges and difficulties. This study demonstrates the potential of combining immersive VTs with oral histories to create engaging educational experiences, preserving the USS Drum’s legacy and making it accessible to a broader audience, including those unable to visit in person. Furthermore, this project sets a precedent for museums to leverage digital tools in preserving and promoting maritime heritage and oral histories.
Genome-Wide Identification of DREB Transcription Factor Family and Functional Analysis of PaDREB1D Associated with Low-Temperature Stress in Phalaenopsis aphrodite
Authors: Hu Ziang , Wang Shuang , Wang Yaoling , Li Jiaming , Luo Ping , Xin Jingjing , Cui Yongyi
Published: September 2024

Low temperatures are the most significant abiotic stressor for the conservation and production of Phalaenopsis in non-tropical areas. CBF/DREB1 transcription factors play an important role in the plant abiotic stress response. In this study, 31 DREB family members were identified in the Phalaenopsis genome. Expression pattern analysis showed that the expression of different PaDREB members varied among tissue sites. PaDREB1D was isolated from Phalaenopsis aphrodite, and multiple sequence alignment showed that PaDREB1D belonged to the A1 subgroup of the DREB family and was localized in the nucleus. PaDREB1D overexpression in protocorm-like bodies of Phalaenopsis reduced cell damage during low-temperature stress, increased antioxidant enzyme activity, and enhanced the low-temperature tolerance of protocorm-like bodies. The results of this study provide a theoretical basis for breeding for cold resistance and investigating the molecular mechanisms related to low-temperature responses in Phalaenopsis.
Biogeography, Conservation Status, and Traditional Uses of Zingiberaceae in Saraburi Province, Thailand, with Kaempferia chaveerachiae sp. nov.
Authors: Boonma Thawatphong , Saensouk Surapon , Saensouk Piyaporn
Published: September 2024

This research investigates the Zingiberaceae family in Saraburi Province, Thailand, aiming to understand its biodiversity, distribution, ecology, conservation status, and traditional uses. Through systematic exploration, 103 species are identified, including a novel species, Kaempferia chaveerachiae Saensouk, P.Saensouk, and Boonma. Additionally, 23 species are newly reported for distribution in Saraburi Province. Methodologically, a district-wise analysis and the unweighted pair group method with arithmetic mean (UPGMA) cluster analysis were employed to discern species prevalence and geographical patterns. Findings reveal varied species distribution aligned with diverse ecological conditions, informing targeted conservation strategies. Fourteen endemic species and the District Endemism Index emphasize localized conservation significance. Assessment of conservation status highlights critical and vulnerable species, prompting focused conservation initiatives. Phenological analysis underscores adaptability through flowering peaks tied to environmental cues. Ethnobotanical insights highlight the multifunctionality of Zingiberaceae, emphasizing its cultural significance and traditional applications. This study offers valuable insights into Zingiberaceae diversity, advocating for tailored conservation strategies and sustainable practices in Saraburi Province to safeguard its unique flora, habitats, and indigenous knowledge.
Serendipita indica: A Promising Biostimulant for Improving Growth, Nutrient Uptake, and Sugar Accumulation in Camellia oleifera
Authors: Fu Wan-Lin , Wu Wei-Jia , Xiao Zhi-Yan , Wang Fang-Ling , Cheng Jun-Yong , Zou Ying-Ning , Hashem Abeer , Abd_Allah Elsayed Fathi , Wu Qiang-Sheng
Published: September 2024

Serendipita indica is a very promising root-associated endophytic fungus that is widely used on various plants; however, whether it affects the growth and physiological activity of an oilseed crop (Camellia oleifera) under field conditions remains unclear. In this study, we analyzed the effects of S. indica inoculation on root colonization rate, growth rate, photosynthetic parameters, mineral element concentrations and related gene expression, and sugar concentrations and expression of their transporter genes in four-year-old C. oleifera trees in the field. The results showed that the root colonization rate of C. oleifera increased from 3.37% to 9.42% following being inoculated with S. indica. Inoculation with S. indica significantly increased the plant height (46.81%), net photosynthetic rate (69.16%), nitrogen balance index (14.44%), chlorophyll index (21.08%), leaf K (7.4%), leaf Ca (13.52%), root P (17.75%), root K (12.80%), soil NH4+-N (17.78%), available K (26.66%), Olsen-P (184.30%), easily extractable glomalin-related soil protein (39.26%), and soil organic carbon (16.25%) concentrations compared to the uninoculated treatment. Inoculation with S. indica also significantly up-regulated the expression of CoHKT1;1 and CoCAX1;2 in the leaves and roots and CoPht1;1, CoPht1;2, and CoPht1;3 in the leaves. Plants inoculated with S. indica also presented significantly higher leaf glucose, fructose, and sucrose concentrations, accompanied by up-regulated expression of CoSWEET2a, CoSWEET7, CoSWEET9b, CoSWEET17a, and CoSWEET17b. These results suggest that S. indica has significant potential as a biostimulant for enhancing the growth and nutritional profile of C. oleifera, thereby contributing to sustainable oilseed production.
The Effects of Planting Density, Training System and Cultivar on Vegetative Growth and Fruit Production in Young Mango (Mangifera indica L.) Trees
Authors: Ibell Paula T. , Normand Frédéric , Wright Carole L. , Mahmud Kare , Bally Ian S. E.
Published: September 2024

Increasing the planting density of mango orchards appears promising for obtaining higher yields, particularly during the first productive years. However, the challenge is to maintain a good balance between vegetative growth and fruit production in the longer term. The objective of this study was to decipher the effects of planting density, training system and cultivar on young mango trees’ growth and production. The experiment, conducted in North Queensland, consisted of five combinations of planting density and training system applied to the cultivars Keitt, Calypso and NMBP-1243. The planting densities were low (208 tree ha−1), medium (416 tree ha−1) and high (1250 tree ha−1). The closed vase conventional training system was applied at each density. Single leader and espalier on trellis training systems were applied at medium and high densities, respectively. The tree canopy dimensions were measured every 6 months from planting, and tree production was recorded from the third to the fifth years after planting. Vegetative growth and fruit production were the results of complex interactions between planting density, training system, cultivar and/or time. The expected increase in orchard yield with higher planting density was observed from the first productive year, despite lower individual tree production at high planting density. Lower vegetative growth and fruit production at high planting density were probably caused by competition between trees. NMBP-1243 and Keitt showed more rapid vegetative growth. Keitt was the most productive cultivar during the first three productive years. The detailed results of this study provide avenues to further explore the behaviour of mango trees at high planting densities.