bluuebunny/crossref_metadata_2025 huggingface dataset:
TIMolol Nasal Spray as a Treatment for Epistaxis in Hereditary Hemorrhagic Telangiectasia (TIM-HHT)—A Prospective, Randomized, Double-Blind, Controlled, Cross-Over Trial
Authors: Andorfer Kornelia E. C. , Zeman Florian , Koller Michael , Zeller Judith , Fischer René , Seebauer Caroline T. , Vielsmeier Veronika , Bohr Christopher , Kühnel Thomas S.
Published: October 2022

To date, there is no approved local therapeutic agent for the treatment of epistaxis due to hereditary hemorrhagic telangiectasia (HHT). Several case reports suggest the topical use of timolol. This monocentric, prospective, randomized, placebo-controlled, double-blinded, cross-over study investigated whether the effectiveness of the standard treatment with a pulsed diode laser can be increased by also using timolol nasal spray. The primary outcome was severity of epistaxis after three months, while the main secondary outcome was severity of epistaxis and subjective satisfaction after one month. Twenty patients were allocated and treated, of which 18 patients completed both 3-month treatment sequences. Timolol was well tolerated by all patients. Epistaxis Severity Score after three months, the primary outcome measure, showed a beneficial, but statistically nonsignificant (p = 0.084), effect of additional timolol application. Epistaxis Severity Score (p = 0.010) and patients’ satisfaction with their nosebleeds after one month (p = 0.050) showed statistically significant benefits. This placebo-controlled, randomized trial provides some evidence that timolol nasal spray positively impacts epistaxis severity and subjective satisfaction in HHT patients when additively applied to standard laser therapy after one month. However, the effect of timolol was observed to diminish over time. Trials with larger sample sizes are warranted to confirm these findings.
Current Applications of Plant-Based Drug Delivery Nano Systems for Leishmaniasis Treatment
Authors: dos Santos Darline B. , Lemos Janaina A. , Miranda Sued E. M. , Di Filippo Leonardo D. , Duarte Jonatas L. , Ferreira Lucas A. M. , Barros Andre L. B. , Oliveira Anna E. M. F. M.
Published: October 2022

Leishmania is a trypanosomatid that causes leishmaniasis. It is transmitted to vertebrate hosts during the blood meal of phlebotomine sandflies. The clinical manifestations of the disease are associated with several factors, such as the Leishmania species, virulence and pathogenicity, the host–parasite relationship, and the host’s immune system. Although its causative agents have been known and studied for decades, there have been few advances in the chemotherapy of leishmaniasis. The urgency of more selective and less toxic alternatives for the treatment of leishmaniasis leads to research focused on the study of new pharmaceuticals, improvement of existing drugs, and new routes of drug administration. Natural resources of plant origin are promising sources of bioactive substances, and the use of ethnopharmacology and folk medicine leads to interest in studying new medications from phytocomplexes. However, the intrinsic low water solubility of plant derivatives is an obstacle to developing a therapeutic product. Nanotechnology could help overcome these obstacles by improving the availability of common substances in water. To contribute to this scenario, this article provides a review of nanocarriers developed for delivering plant-extracted compounds to treat clinical forms of leishmaniasis and critically analyzing them and pointing out the future perspectives for their application.
Fluorescein-Labeled Thiacalix[4]arenes as Potential Theranostic Molecules: Synthesis, Self-Association, and Antitumor Activity
Authors: Akhmedov Alan , Terenteva Olga , Subakaeva Evgenia , Zelenikhin Pavel , Shurpik Ramilia , Shurpik Dmitriy , Padnya Pavel , Stoikov Ivan
Published: October 2022

In this paper, a series of thiacalix[4]arenes were synthesized as potential theranostic molecules for antitumor therapy. We propose an original strategy for the regioselective functionalization of thiacalix[4]arene with a fluorescent label to obtain antiangiogenic agent mimetics. The aggregation properties of the synthesized compounds were determined using the dynamic light scattering. The average hydrodynamic diameter of self-associates formed by the macrocycles in 1,3-alternate conformation is larger (277–323 nm) than that of the similar macrocycle in cone conformation (185–262 nm). The cytotoxic action mechanism of the obtained compounds and their ability to penetrate into of human lung adenocarcinoma and human duodenal adenocarcinoma cells were established using the MTT-test and flow cytometry. thiacalix[4]arenes in 1,3-alternate conformation did not have a strong toxic effect. The toxicity of macrocycles in cone conformations on HuTu-80 and A549 cells (IC50 = 21.83–49.11 µg/mL) is shown. The resulting macrocycles are potential theranostic molecules that combine both the pharmacophore fragment for neoplasmas treatment and the fluorescent fragment for monitoring the delivery and biodistribution of nanomedicines.
A Macrophage Membrane–Polymer Hybrid Biomimetic Nanoplatform for Therapeutic Delivery of Somatostatin Peptide to Chronic Pancreatitis
Authors: Wang Fang , Deng Yu , Yu Luying , Zhou Ao , Wang Jieting , Jia Jingyan , Li Ning , Ding Fadian , Lian Wei , Liu Qicai , Yang Yu , Lin Xinhua
Published: October 2022

The clinical translation of therapeutic peptides is generally challenged by multiple issues involving absorption, distribution, metabolism and excretion. In this study, a macrophage membrane-coated poly(lactic-co-glycolic acid) (PLGA) nanodelivery system was developed to enhance the bioavailability of the somatostatin (SST) peptide, which faces the hurdles of short half-life and potential side effects in the treatment of chronic pancreatitis. Using a facile nanoprecipitation strategy, SST was loaded in the nanoparticles with an encapsulation efficiency (EE) and a loading efficiency (LE) of 73.68 ± 3.56% and 1.47 ± 0.07%, respectively. The final formulation of SST-loaded nanoparticles with the camouflage of macrophage membrane (MP-SST) showed a mean diameter of 151 ± 4 nm and an average zeta potential of −29.6 ± 0.3 mV, which were stable long term during storage. With an above 90% cell viability, a hemolysis level of about 2% (<5%) and a preference for being ingested by activated endothelial cells compared to macrophages, the membrane–polymer hybrid nanoparticle showed biocompatibility and targeting capability in vitro. After being intravenously administered to mice with chronic pancreatitis, the MP-SST increased the content of SST in the serum (123.6 ± 13.6 pg/mL) and pancreas (1144.9 ± 206.2 pg/g) compared to the treatment of (Dulbecco’s phosphate-buffered saline) DPBS (61.7 ± 6.0 pg/mL in serum and 740.2 ± 172.4 pg/g in the pancreas). The recovery of SST by MP-SST downregulated the expressions of chronic pancreatitis-related factors and alleviated the histologic severity of the pancreas to the greatest extent compared to other treatment groups. This augmentation of SST therapeutic effects demonstrated the superiority of integrating the synthetic polymer with biological membranes in the design of nanoplatforms for advanced and smart peptide delivery. Other peptides like SST can also be delivered via the membrane–polymer hybrid nanosystem for the treatment of diseases, broadening and promoting the potential clinical applications of peptides as therapeutics.
Photo-Stimuli-Responsive CuS Nanomaterials as Cutting-Edge Platform Materials for Antibacterial Applications
Authors: Naskar Atanu , Kim Kwang-sun
Published: October 2022

Photo-stimuli-responsive therapeutic nanomaterials have gained widespread attention as frontline materials for biomedical applications. The photoactivation strategies are classified as single-modality (based on either reactive oxygen species (ROS)-based photodynamic therapy (PDT), hyperthermia-based photothermal therapy (PTT)), or dual-modality (which combines PDT and PTT). Due to its minimal invasiveness, phototherapy has been extensively applied as an efficient therapeutic platform for many diseases, including skin cancers. However, extensive implementation of phototherapy to address the emergence of multidrug-resistant (MDR) bacterial infections remains challenging. This review focuses on copper sulfide (CuS) nanomaterials as efficient and cost-effective PDT and PTT therapeutic nanomaterials with antibacterial activity. The features and merits of CuS nanomaterials as therapeutics are compared to those of other nanomaterials. Control of the dimensions and morphological complexity of CuS nanomaterials through judicious synthesis is then introduced. Both the in vitro antibacterial activity and the in vivo therapeutic effect of CuS nanomaterials and derivative nanocomposites composed of 2D nanomaterials, polymers, metals, metal oxides, and proteins are described in detail. Finally, the perspective of photo-stimuli-responsive CuS nanomaterials for future clinical antibacterial applications is highlighted. This review illustrates that CuS nanomaterials are highly effective, low-toxic, and environmentally friendly antibacterial agents or platform nanomaterials for combatting MDR bacterial infections.
Isolation and Molecular Characterization of Two Novel Lytic Bacteriophages for the Biocontrol of Escherichia coli in Uterine Infections: In Vitro and Ex Vivo Preliminary Studies in Veterinary Medicine
Authors: Balcão Victor M. , Belline Bianca G. , Silva Erica C. , Almeida Pablo F. F. B. , Baldo Denicezar Â. , Amorim Lara R. P. , Oliveira Júnior José M. , Vila Marta M. D. C. , Del Fiol Fernando S.
Published: October 2022

E. coli is one of the etiological agents responsible for pyometra in female dogs, with conventional treatment involving ovariohysterectomy. Here, we report the isolation and full characterization of two novel lytic phages, viz. vB_EcoM_Uniso11 (ph0011) and vB_EcoM_Uniso21 (ph0021). Both phages belong to the order Caudovirales and present myovirus-like morphotypes, with phage ph0011 being classified as Myoviridae genus Asteriusvirus and phage ph0021 being classified as Myoviridae genus Tequatrovirus, based on their complete genome sequences. The 348,288 bp phage ph0011 and 165,222 bp phage ph0021 genomes do not encode toxins, integrases or antimicrobial resistance genes neither depolymerases related sequences. Both phages were shown to be effective against at least twelve E. coli clinical isolates in in vitro antibacterial activity assays. Based on their features, both phages have potential for controlling pyometra infections caused by E. coli. Phage ph0011 (reduction of 4.24 log CFU/mL) was more effective than phage ph0021 (reduction of 1.90 log CFU/mL) after 12 h of incubation at MOI 1000. As a cocktail, the two phages were highly effective in reducing the bacterial load (reduction of 5.57 log CFU/mL) at MOI 100, after 12 h of treatment. Both phages were structurally and functionally stabilized in vaginal egg formulations.
Birth Control Use and Access Including Pharmacist-Prescribed Contraception Services during COVID-19
Authors: Pelaccio Karli , Bright David , Dillaway Heather , O’Connell Mary Beth
Published: October 2022

The COVID-19 pandemic influenced health care with effects on contraception access emerging. The study objectives were to analyze pandemic impacts on birth control (BC) use and access; and evaluate perceptions of pharmacist-prescribed BC. A 50-item survey was distributed by 31 Michigan community pharmacies to women aged 18–45 over a three-month period. The survey link was also posted on two websites. 147 surveys were analyzed. Respondents were 29 ± 7.9 years old, primarily white (77%) and straight (81%). Fifty-eight percent of respondents used prescription BC, mostly to prevent pregnancy (84%) with oral pills (76%) being the most common formulation. Some BC users (25%) were worried about BC access and 27% had difficulty taking BC regularly. Half of the respondents (50%) would likely use pharmacist-prescribed BC if available, with advantages being more convenient than visiting a doctor’s office (71%) and easier access (69%). The major concern about pharmacist-prescribed BC was women not receiving PAP smears and screenings (61%). Respondents reported high confidence (72%) in pharmacist-prescribed BC and believe it would help prevent unintended pregnancies (69%). Some respondents experienced altered BC use and access. Half of the respondents supported pharmacist-prescribed BC. Pharmacist-prescribed BC could help solve pandemic-related access problems.
Description of a Pharmacy COVID Champion Service in South East London to Reduce Vaccine Hesitancy
Authors: Micallef Ricarda , Matharu Raj , Barry Abigail , Burgess Vanessa
Published: October 2022

In the United Kingdom, COVID vaccinations were rolled out from December 2020. In July 2021 in South East London there were areas of high COVID prevalence and low vaccination uptake. Therefore, a COVID champion service was launched in community pharmacies enabling pharmacy teams to have conversations with patients regarding the vaccination programme and their concerns and signposting as needed. The aim of the project was to evaluate the impact of the service on COVID vaccination uptake and perceptions of pharmacy teams. Quantitative data was received from pharmacy interaction records plus a pharmacy survey. In addition, qualitative data was received through interviews with 12 pharmacists involved in the service, which was analysed using content analysis, along with interviews with commissioner representatives. Between July and October 2021, 8539 conversations took place. From these 6094 patients agreed to vaccination, with 2019 initially hesitant patients converted. Lack of understanding, risk of blood clots and cultural concerns were the largest areas of hesitance. Pharmacy teams were happy to support conversation and local working with knowledge and confidence. Engagement within the team was the biggest enabler, with pressure to deliver other services being the biggest barrier. These results show the value of community pharmacy teams, having conversations with the public, addressing concerns where applicable, and signposting to the appropriate sites so patients are supported to achieve maximum health outcomes.
Doxycycline Induced Pancreatitis: An Uncommon Complication of a Common Drug
Authors: Reiche William , Abodunrin Faith , Destache Chris , Rangray Rajani , Velagapudi Manasa
Published: October 2022

We describe the risk factors for the development, timing, and severity of doxycycline induced acute pancreatitis (DIAP) in two case reports and a review of prior published cases, to better understand DIAP. Clinicians must maintain a high level of suspicion for DIAP in patients presenting with acute pancreatitis, while on doxycycline therapy. The latency and severity of DIAP are variable, making diagnosis challenging. Treatment includes bowel rest, hydration, and discontinuation of doxycycline.
Artificial Intelligence to Aid Glaucoma Diagnosis and Monitoring: State of the Art and New Directions
Authors: Nunez Roberto , Harris Alon , Ibrahim Omar , Keller James , Wikle Christopher K. , Robinson Erin , Zukerman Ryan , Siesky Brent , Verticchio Alice , Rowe Lucas , Guidoboni Giovanna
Published: October 2022

Recent developments in the use of artificial intelligence in the diagnosis and monitoring of glaucoma are discussed. To set the context and fix terminology, a brief historic overview of artificial intelligence is provided, along with some fundamentals of statistical modeling. Next, recent applications of artificial intelligence techniques in glaucoma diagnosis and the monitoring of glaucoma progression are reviewed, including the classification of visual field images and the detection of glaucomatous change in retinal nerve fiber layer thickness. Current challenges in the direct application of artificial intelligence to further our understating of this disease are also outlined. The article also discusses how the combined use of mathematical modeling and artificial intelligence may help to address these challenges, along with stronger communication between data scientists and clinicians.
Research on High Power Laser Damage Resistant Optically Addressable Spatial Light Modulator
Authors: Du Tongyao , Huang Dajie , Cheng He , Fan Wei , Xing Zhibo , Zhu Jianqiang , Liu Wen
Published: October 2022

Liquid crystal spatial light modulators (LC-SLMs) are devices that can accurately adjust the parameters of beam amplitude, phase, wavefront and polarization. However, due to the limitation of laser damage resistance of component materials, LC-SLMs still have difficulty meeting the application and development needs of a high-average power laser system. Here, we proposed an optically addressable spatial light modulator (OASLM) based on a sapphire substrate. Due to the good thermal conductivity of sapphire, the laser damage resistance of the device was greatly improved. The thermal distribution of OASLM based on the sapphire substrate and the K9 substrate is analyzed by a laser-induced temperature rise model. The experimental results also show the excellent performance of sapphire OASLM under high-power CW laser irradiation, its laser power density is increased from 10 W/cm2 to 75 W/cm2, and the working time is more than 30 min. By bonding sapphire to the other side, the laser power density can be increased to 100 W/cm2, and these are completed without active heat dissipation. This method provides a feasible path for high-average-power SLMs.
Demonstration of Two-Dimensional Beam Steering through Wavelength Tuning with One-Dimensional Silicon Optical Phased Array
Authors: Yoon Hyeonho , Rhee Hyun-Woo , Kwon Nam-Hyun , Kim Jae-Yong , Kim Junhyeong , Yoon Jinhyeong , Park Hyo-Hoon
Published: October 2022

We demonstrate two-dimensional beam steering through wavelength control using a one-dimensional optical phased array (OPA) in which a path difference is built up in each channel to allocate a phase delay sequentially. Prior to the beam steering through wavelength tuning, phase initialization was performed to form a single beam using electro-optic p-i-n phase shifters to compensate for the phase error due to fabrication imperfections. With a 79.6 μm path difference in the phase-feeding lines and a 2 μm pitch in the grating radiators, we achieved a continuous transversal steering of about 46° through a wavelength tuning of about 7 nm. By extending the wavelength tuning range to 90 nm, longitudinal steering was attained near 13° with a discrete interval of about 1°. The beam was maintained during full two-dimensional steering and experienced only a small degree of degradation in the beam divergences and in the side lobe level. We analyzed the parameters to be able to induce the degradation of beam quality considering the fabrication errors of the geometric parameters of the OPA. The results indicated that the scanning scheme employing wavelength tuning after initialization with phase shifters can greatly reduce the realignment process of the beam pattern, even in the presence of some effective index perturbation during the fabrication.
Theoretical Analysis of Optical Properties for Amorphous Silicon Solar Cells with Adding Anti-Reflective Coating Photonic Crystals
Authors: Sayed Hassan , Al-Dossari Mawaheb , Ismail Mohamed A. , Abd El-Gawaad Nashaat S. , Aly Arafa H.
Published: October 2022

In the current study, we aim to limit the power dissipation in amorphous silicon solar cells by enhancing the cell absorbance at different incident angles. The current improvement is justified by adding the single-period of ternary 1D photonic crystal with texturing on the top surface, which acts as an anti-reflecting coating. The texturing shape gives the photons at least two chances to localize inside the active area of the cell. Therefore, it increases the absorbance of the cell. Moreover, we add binary one-dimensional photonic crystals with the features of a photonic band gap, which acts as a back mirror to return the photons that were transmitted inside the cell’s active region. The considered structure is demonstrated by the well-defined finite element method (FEM) by using COMSOL multiphysics.
Surface Depth-Mapping of Material via the Transport-of-Intensity Equation
Authors: Stsepuro Nikita , Kovalev Michael , Krasin George , Podlesnykh Ivan , Gulina Yulia , Kudryashov Sergey
Published: October 2022

We present a new approach for a surface characterization based on the TIE method combined with the SEM. Experimental verification is carried out on the example of characterization of a crater on the surface of monocrystalline silicon (111). The approach is universal and can be used for any opaque object. It improves the robustness and stability of the quantitative phase retrieval process and has two important features. Firstly, it allows one to quantitatively retrieve the phase in a region of arbitrarily chosen dimensions. Secondly, phase retrieval process does not require the choice of boundary conditions.
Supermode Characteristics of Nested Multiple Hollow-Core Anti-Resonant Fibers
Authors: Li Zequan , Liu Jiantao , Xia Changming , Hou Zhiyun , Zhou Guiyao
Published: October 2022

Mode-division multiplexing (MDM) can achieve ultra-high data capacity in optical fiber communication. Several impressive works on multicore fiber (MCF), multi-mode fiber, and few-mode multicore fiber have made significant achievements in MDM. However, none of the previous works can simultaneously maintain the transmission loss, chromatic dispersion (CD), and differential group delay (DGD) at a relatively low level. A nested multiple hollow-core anti-resonant fiber (NMH-ARF) has significant potential for applications in MDM. This study proposes a novel NMH-ARF with its structural design based on the traditional single-core nested anti-resonant fiber. We increased the number of nodes between capillaries. By changing the position of the nested tubes, several interconnected areas form when a single core is separated. We investigated the mode-coupling theory and transmission characteristics of this fiber. This fiber structure showed a low sensitivity to bending and achieved a super-low DGD and a super-low confinement loss (CL) at a wavelength of 1.55 µm while keeping CD relatively low.
Manipulating Transverse Spin Angular Momentum with Symmetrically Modulated Hybridly Polarized Vector Optical Field
Authors: Sun Xue-Feng , Pan Yue , Zhang Guang-Bo , Man Zhong-Xiao , Gao Xu-Zhen
Published: October 2022

Transverse spin angular momentum (SAM) of photon, also known as ‘photonic wheel’, has attracted much attention, owing to its extraordinary properties and broad application prospect. Thus, it is essential to flexibly manipulate the transverse SAM in order to satisfy different needs. In this article, we design and generate a new kind of symmetrically modulated hybridly polarized vector optical field (SM-HP-VOF), and pure transverse SAM can be achieved based on the tightly focused SM-HP-VOF. Through adjusting the parameters of the SM-HP-VOF, the shape, intensity, and symmetry of the transverse SAM can be modulated. Moreover, by adding a segmented vortex phase distribution to the SM-HP-VOF, the transverse SAM becomes more concentrated and stronger, indicating that the orbital angular momentum can be a catalyst in modulating transverse SAM. Such results can provide a new avenue in studying and modulating transverse SAM, which would have potential applications in various areas including chip optical circuitry, optical quantum computing, and optical trapping and manipulation.
Mesoscopic States of Light for the Detection of Weakly Absorbing Objects
Authors: Allevi Alessia
Published: October 2022

Over the past twenty years, different imaging techniques have been proposed and implemented in order to reconstruct the images of different kinds of objects, including faint ones. In this work, we exploit the mesoscopic intensity domain to prove that the determination of the transmittance efficiency of an object can be obtained by considering the calculation of the noise reduction factor in the case of a multi-mode pseudothermal state divided at a balanced beam splitter and detected by photon-number-resolving detectors. The good quality of the experimental results suggests that this strategy can be extended to the determination of a matrix of different transmittance values by means of arrays of photon-number-resolving detectors.
Opportunities and Challenges of Using Feynman Diagrams with Upper Secondary Students
Authors: Dahlkemper Merten Nikolay , Klein Pascal , Müller Andreas , Schmeling Sascha Marc , Wiener Jeff
Published: October 2022

Particle physics is an exciting subject for high school students, and there have been various approaches on how to introduce the topic in the classroom. Feynman diagrams (FDs) are an often-used form of representation in particle physics and could play an important role in such an introduction. However, their potential educational value has not yet been investigated. To this end, we interviewed four experts in the field of particle physics education on the opportunities and challenges Feynman diagrams could pose for high school students. We analyzed their answers using a thematic analysis framework, categorizing them into five themes. The results of these interviews show that there are two challenges (FDs elicit and perpetuate inadequate conceptions about particle physics, and FDs can only be treated superficially in school) and three opportunities (FDs can link particle physics and other physics topics in high school education, FDs offer an opportunity for different particle physics topics to be taught, and FDs offer a connection to current research). The results of this expert interview study lead to several suggestions on how to design learning environments that incorporate Feynman diagrams.
Pre-Sowing Treatment of Tilia cordata Mill. by Seed Material Fermentation
Authors: Bednařík František , Houšková Kateřina , Bezděčková Lena , Mauer Oldřich
Published: October 2022

The aim of the work was to verify whether the fermentation of seed material is a suitable pre-sowing treatment of small-leaved lime. Seed material of two origins was treated for a period of 7, 14 and 21 days using anaerobic fermentation and for 10, 20 and 30 days using aerobic fermentation, the control treatment being the seed after warm–cold stratification. The water content, germination capacity and abscisic acid level of the seed were evaluated, together with the emergence and morphological parameters of annual lime seedlings that had emerged from the tested seeds. It was found that fermented seed had a low abscisic acid level (up to 700 pmol/g), a higher germination capacity (approx. 90%) than mature seed without fermentation and the morphological parameters of the seedlings that had emerged from seed after fermentation reached higher values, compared to those of the seedlings that had emerged from seed after warm–cold stratification. The seed material had to be collected green (e.g., immature seeds) and moistened. A suitable anaerobic fermentation time is 7 days; the aerobic phase can last 10, 20 or 30 days. The fermentation of seed material can shorten the preparation of seed for sowing by 1 year.
Volatile Compounds Governed by Single Recessive Gene Impart Aroma in Sponge Gourd (Luffa cylindrica L. Roem)
Authors: Chaubey Tribhuvan , Sagar Vidya , Singh Ramesh Kumar , Chanotiya Chandan Singh , Pandey Sudhakar , Singh Prabhakar M. , Karmakar Pradip , Singh Jagdish , Singh Bijendra , Singh Dhananjay Pratap , Pandey Koshlendra Kumar , Behera Tusar Kanti
Published: October 2022

As a vegetable crop, sponge gourd is widely consumed worldwide due to its health promoting and nutraceutical value. This study describes genetics of an aromatic genotype VRSG-7-17 and deciphers the genetic control and volatile compound composition of sponge gourd. To study the inheritance of this trait, a cross was made between aromatic light-green-fruited VRSG-7-17 and non-aromatic dark-green-fruited VRSG-194 genotypes. The F1s were found to be non-aromatic and have a green fruit colour. Chi-square (χ2) analysis of backcross and F2 population segregating for aroma suggested that the inheritance of aroma in VRSG-7-17 is governed by a single recessive gene in a simple Mendelian fashion. The SPME–GC/MS analysis of the volatile compounds suggested that the compounds responsible for Basmati rice-like aroma were mainly hexanal, 1-octen-3-ol, 3-octanone and limonene. The aroma persists in the cooked VRSG-7-17 fruits, that did not lose fragrance traits at high temperatures. The inheritance of fruit colour was found to be controlled by a single gene with incomplete dominance. The segregation analysis showed that the aroma and fruit colour were not linked, and they segregated independently. The findings will lead to understanding the inheritance of the aromatic compounds in the sponge gourd and may be utilised in the breeding programmes for developing improved aromatic varieties.
Silicon Supplementation Alleviates Adverse Effects of Ammonium on Ssamchoo Grown in Home Cultivation System
Authors: Noh Kyungdeok , Jeong Byoung Ryong
Published: October 2022

Ssamchoo is recently attracting attention as a household hydroponic vegetable in Korea. It has a refreshing texture and a rich content of vitamins and fiber. Ssamchoo with a wide leaf area is suitable for traditional ssam or vegetable wraps, as well as a vegetable for salads; thus, it can be used in a variety of dishes. However, Ssamchoo plants responds sensitively to the nutrient solution, and it is often difficult to secure sufficient leaf area and robust growth using a commercial nutrient solution for leafy vegetables. This study consisted of three experiments conducted to develop the nutrient solution for Ssamchoo grown in a newly developed home hydroponic cultivation system using light-emitting diodes as the sole source of light. In the first experiment, growth and development of Ssamchoo in a representative commercial nutrient solution, Peters Professional (20-20-20, The Scotts Co., Marysville, OH, USA), was compared with laboratory-prepared nutrient solutions, GNU1 and GNU2. As a result, the Ssamchoo grown in Peters Professional had a high NH4+ content in the tissue, leaf yellowing, darkened root color, and suppressed root hair development. In addition, adverse effects of ammonium such as low fresh weight and shorter shoot length were observed. In the second experiment, Peters Professional was excluded, and the ratio of NO3− to NH4+ in the GNU1 and GNU2 nutrient solutions was set to four levels each (100:0, 83.3:16.7, 66.7:33.3, and 50:50). As a result, the fresh weights of 83.3:16.7 and 66.7:33.3 were the greatest, and the leaf color was a healthy green. However, at 100:0 and 50:50 NO3−/NH4+ ratios, the fresh weight was low, and leaf yellowing, tip burn, and leaf burn appeared. The nutrient solution with a 83.3:16.7 NO3−- to-NH4+ ratio, which gave the greatest fresh weight in the second experiment, was chosen as the control, while the solution with a 50:50 NO3−/NH4+ ratio with a lower nitrate content among the two unfavorable treatments was selected as a treatment group for the next experiment. In the third experiment, NH4+ was partially replaced with urea to make four different ratios of NO3− to NH4+ to urea (83:17:0, 50:50:0, 50:25:25, and 50:0:50) in combination with two levels of Si (0 and 10.7 mmol·L−1 Si). The greatest fresh weight was obtained in the treatment in which the NO3−/NH4+/urea ratio was 50:25:25. In particular, when Si was added to the solution, there was no decrease in the number of leaves, and plants with the greatest fresh weight, chlorophyll content, and leaf area were obtained. The number of leaves and leaf area are important indicators of high productivity since the Ssamchoo is used in ssam dishes. It can be concluded that a solution with a NO3−/NH4+/urea ratio of 50:25:25 and supplemented with 10.7 mmol·L−1 Si is the most suitable nutrient solution for growing Ssamchoo in the home hydroponic system developed.
Plant Age and Soil Texture Rather Than the Presence of Root Hairs Cause Differences in Maize Resource Allocation and Root Gene Expression in the Field
Authors: Ganther Minh , Lippold Eva , Bienert Manuela Désirée , Bouffaud Marie-Lara , Bauer Mario , Baumann Louis , Bienert Gerd Patrick , Vetterlein Doris , Heintz-Buschart Anna , Tarkka Mika Tapio
Published: October 2022

Understanding the biological roles of root hairs is key to projecting their contributions to plant growth and to assess their relevance for plant breeding. The objective of this study was to assess the importance of root hairs for maize nutrition, carbon allocation and root gene expression in a field experiment. Applying wild type and root hairless rth3 maize grown on loam and sand, we examined the period of growth including 4-leaf, 9-leaf and tassel emergence stages, accompanied with a low precipitation rate. rth3 maize had lower shoot growth and lower total amounts of mineral nutrients than wild type, but the concentrations of mineral elements, root gene expression, or carbon allocation were largely unchanged. For these parameters, growth stage accounted for the main differences, followed by substrate. Substrate-related changes were pronounced during tassel emergence, where the concentrations of several elements in leaves as well as cell wall formation-related root gene expression and C allocation decreased. In conclusion, the presence of root hairs stimulated maize shoot growth and total nutrient uptake, but other parameters were more impacted by growth stage and soil texture. Further research should relate root hair functioning to the observed losses in maize productivity and growth efficiency.
Bioassay Guided Fractionation Protocol for Determining Novel Active Compounds in Selected Australian Flora
Authors: Mani Janice , Johnson Joel , Hosking Holly , Hoyos Beatriz , Walsh Kerry , Neilsen Paul , Naiker Mani
Published: October 2022

A large variety of unique and distinct flora of Australia have developed exceptional survival methods and phytochemicals and hence may provide a significant avenue for new drug discovery. This study proposes a bioassay guided fractionation protocol that maybe robust and efficient in screening plants with potential bioactive properties and isolating lead novel compounds. Hence, five native Australian plants were selected for this screening process, namely Pittosporum angustifolium (Gumbi gumbi), Terminalia ferdinandiana (Kakadu plum, seeds (KPS), and flesh (KPF)), Cupaniopsis anacardioides (Tuckeroo, seeds (TKS) and flesh (TKF)), Podocarpus elatus (Illawarra plum, seeds (IPS) and flesh (IPF)) and Pleiogynium timoriense (Burdekin plum, seeds (BPS) and flesh (BPF)). The methanolic extracts of the plants samples were analysed for Total phenolic content (TPC) and antioxidant capacity measure by FRAP. The highest values were found in the KPF which were 12,442 ± 1355 mg GAE/ 100 g TPC and 16,670 ± 2275 mg TXE/100 g antioxidant capacity. Extracts of GGL was deemed to be most potent with complete cell inhibition in HeLa and HT29, and about 95% inhibition in HuH7 cells. Comparative activity was also seen for KPS extract, where more than 80% cell inhibition occurred in all tested cell lines. Dose-dependent studies showed higher SI values (0.72–1.02) in KPS extracts than GGL (0.5–0.73). Microbial assays of the crude extracts were also performed against five bacterial strains commonly associated with causing food poisoning diseases were selected (Gram positive—Staphylococcus aureus and Gram negative—Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa bacteria). KPF extracts were effective in suppressing microbial growth of all tested bacterial strains except for P. aeruginosa, while TKS and TKF were only slightly effective against S. aureus. Due to the potential of the GGL crude extract to completely inhibit the cells compared to KPS, it was further fractionated and tested against the cell lines. HPLC phenolic profiling of the crude extracts were performed, and numerous peak overlaps were evident in the fruit extracts. The KPF extracts demonstrated the strongest peaks which was coherent with the fact that it had the highest TPC and antioxidant capacity values. A high occurrence of t-ferulic acid in the GGL extracts was found which may explain the cytotoxic activity of GGL extracts. Peaks in KPS and KPF extracts were tentatively identified as gallic acid, protocatechuic acid, 4-hydroxybenzoic acid and syringic acid and possibly ellagic acid. HPLC time-based fractionation of the GGL extract (F1–F5) was performed and Dose dependent cytotoxic effects were determined. It was construed that F1, having the highest SI value for HeLa, HT29 and HuH7 (1.60, 1.41 and 1.67, respectively) would be promising for further fractionation and isolation process.
Seed Weight and Trade-Offs: An Experiment in False Rhodes Grasses under Different Aridity Conditions
Authors: Marinoni Lorena , Zabala Juan M. , Quiroga R. Emiliano , Richard Geraldina A. , Pensiero José F.
Published: October 2022

The false Rhodes grasses [Leptochloa crinita (Lag.) P.M. Peterson and N.W. Snow and Leptochloa pluriflora (E. Fourn.) P.M. Peterson and N.W. Snow] are considered valuable native forage resources for arid and semiarid rangelands in Argentina and the United States. Effectively using plant materials as forage under aridity conditions requires understanding their resource allocation under those conditions. In the present study, plant functional traits were evaluated in six populations of each false Rhodes grass species from different geographic origin in a humid and an arid region. The evaluation was focused on seed weight, due to the key role of this trait in plant survival. The implication of seed weight in germination under osmotic stress and trade-off relationships between functional traits were also analysed. A fixed ontogenetic variation was found in both species, since populations maintained a stable seed weight across environments. The tolerance to osmotic stress at germination stage was more related to seed weight than to population origin or maternal environment of seeds; heavier-seeded populations produced heavier seedlings instead of a higher number of germinated seeds or higher germination rates. Some traits varied between environments but other traits exhibited a fixed response. Variation patterns among populations were similar within environments and in some cases even for populations from the same geographic origin, revealing a fixed ontogenetic variation; this phenomenon was clearer in L. crinita than in L. pluriflora. Moreover, several different trade-off strategies were detected in both species. These results reinforce the knowledge about the key role of seed weight in survival and performance of seedlings at initial growth stages under arid conditions; however, at advanced stages, other traits would have an important function in growth and development of false Rhodes grasses.
Expression and Variation of the Genes Involved in Rhizobium Nodulation in Red Clover
Authors: Dinkins Randy D. , Hancock Julie A. , Bickhart Derek M. , Sullivan Michael L. , Zhu Hongyan
Published: October 2022

Red clover (Trifolium pratense L.) is an important forage crop and serves as a major contributor of nitrogen input in pasture settings because of its ability to fix atmospheric nitrogen. During the legume-rhizobial symbiosis, the host plant undergoes a large number of gene expression changes, leading to development of root nodules that house the rhizobium bacteria as they are converted into nitrogen-fixing bacteroids. Many of the genes involved in symbiosis are conserved across legume species, while others are species-specific with little or no homology across species and likely regulate the specific plant genotype/symbiont strain interactions. Red clover has not been widely used for studying symbiotic nitrogen fixation, primarily due to its outcrossing nature, making genetic analysis rather complicated. With the addition of recent annotated genomic resources and use of RNA-seq tools, we annotated and characterized a number of genes that are expressed only in nodule forming roots. These genes include those encoding nodule-specific cysteine rich peptides (NCRs) and nodule-specific Polycystin-1, Lipoxygenase, Alpha toxic (PLAT) domain proteins (NPDs). Our results show that red clover encodes one of the highest number of NCRs and ATS3-like/NPDs, which are postulated to increase nitrogen fixation efficiency, in the Inverted-Repeat Lacking Clade (IRLC) of legumes. Knowledge of the variation and expression of these genes in red clover will provide more insights into the function of these genes in regulating legume-rhizobial symbiosis and aid in breeding of red clover genotypes with increased nitrogen fixation efficiency.
Synergistic Effects of Trichoderma harzianum, 1,3 Dichloropropene and Organic Matter in Controlling the Root-Knot Nematode Meloidogyne incognita on Tomato
Authors: d’Errico Giada , Greco Nicola , Vinale Francesco , Marra Roberta , Stillittano Virgilio , Davino Salvatore Walter , Woo Sheridan Lois , D’Addabbo Trifone
Published: October 2022

Environmental concerns raised by synthetic nematicides are encouraging integrated management strategies based on their combination with non-chemical control tools, such as biocontrol agents and/or organic amendments. In this study, the combination of the fumigant 1,3-dichloropropene (1,3-D) with a commercial formulation of the biocontrol agent Trichoderma harzianum (TH) and an organic fertilizer (OF) was investigated in two consecutive tomato crops for its effect on the root-knot nematode Meloidogyne incognita and plant growth and yield. The application of 1,3-D was only performed on the first crop, while TH and OF were provided to both crops. Almost all treatments significantly reduced nematode infestation in both crops, though the greatest nematicidal effect was caused by a combination of the three products. The treatment with 1,3-D limited its nematicidal efficacy to the first crop only. Fumigant integration with TH and OF also resulted in the greatest increases of plant growth and yield. Therefore, the integrated management of root-knot nematodes with a soil fumigant, a bionematicide as T. harzianum and a source of organic matter demonstrated effective nematode suppression though limiting the number of chemical applications.
Agents Affecting the Plant Functional Traits in National Soil and Water Conservation Demonstration Park (China)
Authors: Duan Gaohui , Wen Zhongming , Xue Wei , Bu Yuankun , Lu Jinxin , Wen Bojin , Wang Boheng , Chen Sihui
Published: October 2022

Plant functional traits (PFTs) can reflect the response of plants to environment, objectively expressing the adaptability of plants to the external environment. In previous studies, various relationships between various abiotic factors and PFTs have been reported. However, how these factors work together to influence PFTs is not clear. This study attempted to quantify the effects of topographic conditions, soil factors and vegetation structure on PFTs. Four categories of variables were represented using 29 variables collected from 171 herb plots of 57 sites (from different topographic and various herb types) in Xindian SWDP. The partial least squares structural equation modeling showed that the topographic conditions and soil properties also have a direct effect on plant functional traits. Among the topographic conditions, slope (SLO) has the biggest weight of 0.629, indicating that SLO contributed the most to plant functional traits and vegetation structure. Among soil properties, maximum water capacity (MWC) contributes the most and is followed by soil water content (SWC), weighted at 0.588 and 0.416, respectively. In a word, the research provides new points into the quantification of the correlation between different drivers that may be important for understanding the mechanisms of resource utilization, competition and adaptation to the environment during plant recovery.
Soil Fungal Composition Drives Ecosystem Multifunctionality after Long-Term Field Nitrogen and Phosphorus Addition in Alpine Meadows on the Tibetan Plateau
Authors: Cheng Bingheng , Liu Hongyan , Bai Juan , Li Jinhua
Published: October 2022

An ecosystem can provide multiple functions and services at the same time, i.e., ecosystem multifunctionality (EMF). Above- and belowground biodiversity and abiotic factors have different effects on EMF. Human activities increase atmospheric nitrogen (N) and phosphorus (P) deposition, but the mechanism of how atmospheric N and P deposition affect EMF in alpine meadows on the Tibetan Plateau is still unclear. Here, we measured eleven ecosystem parameters to quantify EMF by averaging method and explored the impact of plant and microbial species diversity and abiotic factors on EMF after long-term field N and P addition in alpine meadows on the Tibetan Plateau. Results showed that N addition reduced EMF by 15%, NP increased EMF by 20%, and there was no change due to P addition. N and P addition reduced pH, relative light conditions (RLC), and plant species richness and modified plant and fungal community composition. Structural equation model (SEM) analysis confirmed that fungal community composition was an important and positive driver on EMF. These results provided an understanding of how N and P addition affect EMF directly and indirectly through biotic and abiotic pathways, which was important for predicting the response of EMF to atmospheric N and P deposition in the future. Furthermore, the findings suggested that soil fungal composition was more important driving factors than abiotic factors in the response of EMF to N and P addition and the importance of the interactions between plant and soil microbial species diversity in supporting greater EMF.
Color Variation and Secondary Metabolites’ Footprint in a Taxonomic Complex of Phyteuma sp. (Campanulaceae)
Authors: Grohar Mariana Cecilia , Medic Aljaz , Ivancic Tea , Veberic Robert , Jogan Jernej
Published: October 2022

In the genus Phyteuma, the taxonomic delimitation of some species is difficult since a high variability of morphological traits, such as flower color, is present, probably due to high levels of hybridization. Historic descriptions and the morphological traits used in the taxonomic keys are sometimes unclear and lead to misinterpretations. Here, a detailed analysis of flower color variability in different populations of sympatric P. spicatum, P. ovatum, and P. persicifolium constitutes a new approach to clarifying the taxonomic statuses. The numeric analysis of color, providing colorimetric variables, together with the detailed description of the metabolic profiles of populations with different flower colors, constitute a unique chemical fingerprint that identifies species and subspecies with clear markers. This study is the most complete metabolic research on genus Phyteuma, since we identified and quantified 44 phenolic compounds using HPLC-MS, comprising 14 phenolic acids, 23 flavonols and flavones, and, for the first time in the genus, 7 anthocyanins involved in flower color variability. This approach contributes to clarifying the differences between species, which is particularly relevant in taxonomic complexes such as the present, where morphology fails to clearly differentiate taxa at specific and intraspecific levels.
Cannabinol: History, Syntheses, and Biological Profile of the Greatest “Minor” Cannabinoid
Authors: Maioli Chiara , Mattoteia Daiana , Amin Hawraz Ibrahim M. , Minassi Alberto , Caprioglio Diego
Published: October 2022

Cannabis (Cannabis sativa L.) is an outstanding source of bioactive natural products, with more than 150 different phytocannabinoids isolated throughout the decades; however, studies of their bioactivity have historically concentrated on the so-called “big four” [∆9-THC (1a), CBD (2a), CBG (3a) and CBC (4a)]. Among the remaining products, which have traditionally been referred to as “minor cannabinoids”, cannabinol (CBN, 5a) stands out for its important repercussions and implications on the global scientific landscape. Throughout this review, we will describe why CBN (5a) deserves a prominent place within the so-called “cannabinome”, providing an overview on its history, the syntheses developed, and its bioactivity, highlighting its promising pharmacological potential and the significant impact that the study of its chemistry had on the development of new synthetic methodologies.