bluuebunny/crossref_metadata_2025 huggingface dataset:
Social Cohesion and Community Resilience during the COVID-19 Pandemic in Northern Romania
Authors: Saghin Despina , Lupchian Maria-Magdalena , Lucheș Daniel
Published: April 2022

The COVID-19 pandemic and the lock-down have highlighted the growing awareness of the need to involve the population in solving problems that directly affect the existence and trajectory of the life of the individual and civil society in the local, national, and regional context. The article aims both to analyze the reaction of formal and informal civil society in a context of major crisis and to analyze how the population perceives the involvement of civil society at the level of a county in Romania and its county seat city. The present sociological diagnosis used data that were collected through an online survey at the beginning of May 2020 among the population of Suceava County. It was sought to identify how the reaction of civil society was perceived and how it was mobilized, as well as how the mass media contributed to reducing the effects of the pandemic. After the elimination phase of non-compliant responses, the volume of the sample included a total of 1231 people. The results of the study indicate that this pandemic context, which manifested as a major crisis, also had positive effects in the sense of mobilizing latent but extensive energies at the local level, whose manifestation contributed to diminishing and limiting the effects of the sanitary crisis the county faced. The media, as a component of civil society, has managed to mobilize important segments of the population, both in quarantined localities and in other localities in Suceava County and Moldova. The COVID-19 crisis tested the social cohesion and resilience of communities and offered perhaps one of the most remarkable lessons of solidarity in the post-December period, both locally and nationally. Although the perception of Romanians on the role of civil society would rather be part of a culture of individualism, in extreme situations it was found that its activity has never been more important.
Characteristics of Pacing Strategies among Elite Cross-Country Skiers According to Final Rank
Authors: Vikestad Vidar , Dalen Terje
Published: April 2022

The purpose of this study is to explore differences in pacing strategies between successful and less successful male elite cross-country skiers during a 15 km interval-start race involving different techniques. The final rank, split times and final times were extracted individually for the top 100 finishers in the 15 km individual time trial races from the Norwegian national season opener races over two years. The same course was used in all the competitions. The athletes were divided into four groups according to final rank: Q1: 1st–25th; Q2: 26th–50th; Q3: 51st–75th; Q4: 76th–100th. The relative change in speed was used for the time spent on lap 1, to an average for laps 2 and 3. Significant correlation between placement and speed reduction after the first lap was found in three out of four races. In Race 2 (skating), both Q1 and Q2 had lower speed decreases between laps than Q4 did. In year 2, both races (classical and skating) had lower speed reduction between laps for the first quartile compared to that of the last. Overall, this study shows that lower-level cross-country skiers started out relatively faster in the first lap and achieved a greater reduction in speed in the subsequent laps when compared to their faster opponents.
Segregating Suspected Child Maltreatment from Non-Child Maltreatment Injuries: A Population-Based Case-Control Study in Taiwan
Authors: Jin Yo-Ting , Chen Chin-Mi , Huang Yao-Ching , Chung Chi-Hsiang , Sun Chien-An , Huang Shi-Hao , Chien Wu-Chien , Wu Gwo-Jang
Published: April 2022

Objective: To identify the differential patient characteristics, injury types, and treatment outcomes between hospitalized child abuse and non-child abuse injuries in Taiwan. Methods: Using the data from the National Health Insurance Research Database, we selected a total of 1525 patients under the age of 18 that were diagnosed with child abuse, as well as an additional 6100 patients as a comparison group. Chi-square test, Fisher exact test, and independent samples t-test were used to compare the differences between the abused children and the non-abuse-related injured children. The multivariate conditional logistic regression was performed to measure the risk factor of child maltreatment in injured children. Results: Intracranial injury was more frequent in the child abuse group than it was in the non-child abuse group (35.0% vs. 8.2%; p < 0.001). Children in the child abuse group tended to stay longer in the hospital and incur higher medical expenses (8.91 days vs. 4.41 days and USD 2564 vs. USD 880, respectively). In multivariate analysis, the Adjusted Odds Ratio (Adjusted OR) of abuse resulting in an injury for children in low-income families is 1.965 times higher than those in non-low-income families (p < 0.001). Children living in high urbanization areas had a significantly higher probability of being abused than those living in low urbanization areas (p < 0.001). Conclusion: Children under the age of 1 who were hospitalized with severe intracranial injuries are highly at risk for child maltreatment. Moreover, numerous high-risk environmental factors were observed in child abuse cases, including living in urban areas, families with low income, and seasonality, as child maltreatment cases occur more frequently in autumn.
Activity Level and Nature of Practice and Play in Children’s Football
Authors: Nybakken Tone , Falco Coral
Published: April 2022

This study analyzes the activity level and nature of organized football training (deliberate practice, DPR), compared with when children play football on their own (deliberate play, DPL), in a sample of selected (YT) and non-selected (BT) talents. A total of 29 observations were analyzed over 2650 min, focusing on the kind of activity, variability, and intensity of the training. In DPL, there are more finishing on goal, involvement, and challenges in 1:1 situation, and more ball touches and ball transport in games, compared with DPR. Additionally, DPL has more activity time (68% vs. 56%) and fewer breaks overall (32% vs. 44%). In DPL, children spend more time playing against each other (92% vs. 36%), and most of the time there are games or finishing on goal. In DPR, children spend more time playing together with someone (2% vs. 44%) and in passing and receiving the ball. DPR training contains more standardized exercises and protected situations. DPR-YT training differs from DPR-BT training with less activity time, ball touches, attempts on goal, and 1:1 situations. In conclusion, the results support DPL providing more football-specific activity. More DPR training at the expense of DPL might reduce practice time for skill development.
How Will Video Conference Fatigue Affect Participants of MICE in the With-COVID-19 Era? Focusing on Video Conference Quality, Social Presence Theory, and Flow
Authors: Jang Mi-Hwa , Choi Eui-Yul
Published: April 2022

Is our mental health at risk due to spending a significant amount of time online due to the COVID-19 pandemic? In the new era that we are living in, where we live a life that coexists with the virus, we are participating in video conferences held online rather than on-site in order to slow the spread of the virus. Video conferencing has become our necessity since March 2020, and is becoming a new standard, especially in the MICE industry. Recently, however, people who have excessively used video conference platforms are complaining of video conference fatigue, which is a new negative emotion such as stress, anxiety, and worry as well as general work fatigue. Therefore, this study focused on the mechanism of video conferencing in MICE, which is rapidly digitally converted by the virus, and the digital psychological factors of the participants. This study derived the quality attributes of video conferencing in MICE and empirically analyzed the relationship with digital psychological factors of the video conference participants, such as video conference fatigue, social presence, and flow. One hundred and thirty-eight valid questionnaires collected from participants of several international academic conferences held in EXCO, Daegu, Korea, from 23 to 28 May 2021, were analyzed. The main results are as follows. First, unlike general video conference fatigue, MICE video conference fatigue was not found to be related to the preceding and following variables. This is due to the characteristics of the MICE video conference and the expertise of the participants. Second, social presence was identified as an important variable in MICE video conferencing. Although media-mediated, the feeling of being present with the presenter and participants was found to affect the participants’ flow in the video conference. Third, in this study, the fun factor was identified as the most important video conference quality that can enhance the social presence of the video conference participants of MICEs.
Using the TUG Test for the Functional Assessment of Patients with Selected Disorders
Authors: Graff Krzysztof , Szczerbik Ewa , Kalinowska Małgorzata , Kaczmarczyk Katarzyna , Stępień Agnieszka , Syczewska Małgorzata
Published: April 2022

One of the tests used for quantitative diagnostics is Timed Up-and-Go (TUG), however, no reports were found regarding the percentage share of individual test components, which seems to have a greater diagnostic value in differentiating the functional status of the patients. The aim of the study was to analyze the percentage of the individual components of the TUG test in functional assessment in a population of healthy children and in clinical trials patients with various diseases. Material and Methodology. The material consisted of patients with orthopedic (n = 165), metabolic (n = 116) and neurological dysfunctions (n = 96). Results. The components of the TUG test that differentiated the studied groups of patients to the greatest extent were in the order: relapse tug3%, initial transition tug2%, sitting tug5% and standing up tug1%, while during the final transition tug4% statistically significant differences were found only between healthy children and the studied groups of patients. Conclusions. The TUG test turned out to be a good diagnostic tool, differentiating the studied groups of patients. The analysis of the percentage of the components of the TUG test can help in assessing the mobility of children and adolescents, monitor the effects of physiotherapy or the effects of surgical procedures.
Nutritional and Exercise Interventions in Cancer-Related Cachexia: An Extensive Narrative Review
Authors: Clemente-Suárez Vicente Javier , Redondo-Flórez Laura , Rubio-Zarapuz Alejandro , Martínez-Guardado Ismael , Navarro-Jiménez Eduardo , Tornero-Aguilera José Francisco
Published: April 2022

One of the common traits found in cancer patients is malnutrition and cachexia, which affects between 25% to 60% of the patients, depending on the type of cancer, diagnosis, and treatment. Given the lack of current effective pharmacological solutions for low muscle mass and sarcopenia, holistic interventions are essential to patient care, as well as exercise and nutrition. Thus, the present narrative review aimed to analyze the nutritional, pharmacological, ergonutritional, and physical exercise strategies in cancer-related cachexia. The integration of multidisciplinary interventions could help to improve the final intervention in patients, improving their prognosis, quality of life, and life expectancy. To reach these aims, an extensive narrative review was conducted. The databases used were MedLine (PubMed), Cochrane (Wiley), Embase, PsychINFO, and CinAhl. Cancer-related cachexia is a complex multifactorial phenomenon in which systemic inflammation plays a key role in the development and maintenance of the symptomatology. Pharmacological interventions seem to produce a positive effect on inflammatory state and cachexia. Nutritional interventions are focused on a high-energy diet with high-density foods and the supplementation with antioxidants, while physical activity is focused on strength-based training. The implementation of multidisciplinary non-pharmacological interventions in cancer-related cachexia could be an important tool to improve traditional treatments and improve patients’ quality of life.
A Randomized Controlled Trial of a Parent Management Training Program for Incarcerated Parents: Post-Release Outcomes
Authors: Eddy J. Mark , Martinez Charles R. , Burraston Bert O. , Herrera Danita , Newton Rex M.
Published: April 2022

The majority of incarcerated adults are parents. While in prison, most parents maintain at least some contact with their families. A positive connection with family during imprisonment is hypothesized to improve long-term success after release. One way in which departments of corrections attempt to facilitate positive connections with family is through prison-based parenting programs. One such program, developed in collaboration with the Oregon Department of Corrections, is the cognitive-behavioral parent management training program Parenting Inside Out (PIO). Outcomes due to PIO were examined within the context of a randomized controlled trial. Incarcerated parents from all correctional facilities in the state of Oregon were recruited to participate, and eligible parents who consented (N = 359) were transferred to participating releasing institutions. After initial assessment, parents were randomized to condition (i.e., PIO “intervention” condition or services-as-usual “control” condition) and then followed through the remainder of their prison sentences and to one year after release. Intervention condition participants were offered PIO prior to their release. Outcomes favoring participants in the intervention condition were found in areas of importance to parents and their children and families and to public health and safety at large, including a decreased likelihood of problems related to substance use and of engaging in criminal behavior during the first six months following release as well as a decreased likelihood of being arrested by police during the first year following release. The implications of the findings are discussed, including the critical need for scientifically rigorous research on multi-component parenting programs delivered during the reentry period.
Exploring an Efficient Evolutionary Game Model for the Government–Enterprise–Public during the Double Carbon Policy in China
Authors: Zou Bilin , Ju Chunhua , Bao Fuguang , Lai Ye , Xu Chonghuan , Zhu Yiwen
Published: April 2022

The realization of China’s “double carbon” goal is of great significance to the world environment and China’s economy and society. Through the establishment of the “government–enterprise–public” evolutionary game model, this paper explores the interaction between government policy guidance, low-carbon technology R&D behavior of enterprises, and public purchase of carbon label products, as well as the micro-driving path, aiming to provide suggestions for the implementation of the “double carbon” policy and carbon label system in China. The results show that the choice of government, enterprises, and public strategies is closely related to their own costs and benefits. Public sentiment can effectively urge the government to actively fulfill its responsibilities. Effective government policy guidance plays a key role in low-carbon technology R&D behavior of enterprises. There is an interaction between low-carbon technology R&D behavior of enterprises and public purchase of carbon label products.
Reply to Moretti et al. Would Moving Forward Mean Going Back? Comment on “Maselli et al. Direct Access to Physical Therapy: Should Italy Move Forward? Int. J. Environ. Res. Public Health 2022, 19, 555”
Authors: Maselli Filippo , Piano Leonardo , Cecchetto Simone , Storari Lorenzo , Rossettini Giacomo , Mourad Firas
Published: April 2022

We want to thank you for the opportunity to respond to the issues raised in the letter to the Editor of Dr. Moretti et al. [...]
Comovement across BRICS and the US Stock Markets: A Multitime Scale Wavelet Analysis
Authors: Batondo Musumba , Uwilingiye Josine
Published: April 2022

During the past two decades, financial markets across the globe have experienced sporadic waves of crashes. Such waves raise concerns about the vulnerability of global financial markets and the transmission mechanisms of shocks beyond borders. The current study examines the co-movement of stock markets in BRICS (Brazil, Russia, India, China and South Africa) countries and the United States of America (US). It unfolds their exposure to contagion effects during the major financial crises, which have flared up since 2000. Daily close price indices of selected stock markets were used in this endeavour. These data spanned from 5 January 2000 to 10 March 2021. A wavelet decomposition on stock return series was performed on these data to determine the multihorizon nature of comovement (pure contagion or interdependence) and the dynamics of market integration. It emerges that before the 2006-US-housing-bubble and after the 2011/13-EU-sovereign-debt crises, some shocks caused pure contagion. Such transmission generated short-term shocks. Most of the earlier shocks, particularly the US subprime and the EU Sovereign Debt crises, were spread via interdependence. Trade linkages and economic integration improvements enhanced such interdependence. In addition, when analysing the episodes of market integration, it arises that, in general, the short- and long-term integration strengthened and deepened comovement among equity markets. From the portfolio diversification and risk management perspectives, these results indicate that the market in China provided lucrative grounds for short-run investors from the other countries covered in the current study. These results can be helpful for investors interested in portfolio diversification in the BRICS region. They might also help policymakers in the region mitigate the exposure to external shocks of markets.
Tadalafil and Steroid Hormones Interactions in Adipose, Bone and Prostate Tissues: Focus on Translational Perspectives
Authors: Greco Emanuela Alessandra , Antinozzi Cristina , Di Luigi Luigi , Aversa Antonio , Sgrò Paolo
Published: April 2022

Tadalafil is a selective phosphodiesterase type-5 (PDE5) inhibitor that is approved for the treatment of men with erectile dysfunction (ED) and/or benign prostate hyperplasia (BPH) -associated symptoms. Besides its classical actions on PDE5 within the genitourinary tract, where the specific enzyme expression is maximal, it may exert different systemic effects. This is mainly due to the pleiotropic distribution of PDE5 enzyme throughout the human (and animal) body, where it can exert protective effects in different clinical conditions. Recently, it has been demonstrated that tadalafil may display novel actions on androgen receptor (AR) expression and activity and cytochrome P19a1 (Cyp19a1) and estrogen receptor β (ERβ) expression in different in vitro systems, such as adipose, bone and prostate cancer cells, where it can act as a selective modulator of steroid hormone production. This may determine novel potential mechanism(s) of control in pathophysiologic pathways. In this review, we summarize basic research and translational results applicable to the use of tadalafil in the treatment of obesity, bone loss and prostate cancer.
Charged Residue Implantation Improves the Affinity of a Cross-Reactive Dengue Virus Antibody
Authors: Wei Huiling , Tan Jie , Zhou Bingjie , Guan Xiaotong , Zhong Qiaoxian , Wang Jiaqi
Published: April 2022

Dengue virus (DENV) has four serotypes that complicate vaccine development. Envelope protein domain III (EDIII) of DENV is a promising target for therapeutic antibody development. One EDIII-specific antibody, dubbed 1A1D-2, cross-reacts with DENV 1, 2, and 3 but not 4. To improve the affinity of 1A1D-2, in this study, we analyzed the previously solved structure of 1A1D-2-DENV2 EDIII complex. Mutations were designed, including A54E and Y105R in the heavy chain, with charges complementary to the epitope. Molecular dynamics simulation was then used to validate the formation of predicted salt bridges. Interestingly, a surface plasmon resonance experiment showed that both mutations increased affinities of 1A1D-2 toward EDIII of DENV1, 2, and 3 regardless of their sequence variation. Results also revealed that A54E improved affinities through both a faster association and slower dissociation, whereas Y105R improved affinities through a slower dissociation. Further simulation suggested that the same mutants interacted with different residues in different serotypes. Remarkably, combination of the two mutations additively improved 1A1D-2 affinity by 8, 36, and 13-fold toward DENV1, 2, and 3, respectively. In summary, this study demonstrated the utility of tweaking antibody-antigen charge complementarity for affinity maturation and emphasized the complexity of improving antibody affinity toward multiple antigens.
Fluorine-Containing Drug Administration in Rats Results in Fluorination of Selected Proteins in Liver and Brain Tissue
Authors: Gawor Andrzej , Gajewski Zdzislaw , Paczek Leszek , Czarkowska-Paczek Bozena , Konopka Anna , Wryk Grzegorz , Bulska Ewa
Published: April 2022

In many pharmaceuticals, a hydrogen atom or hydroxyl group is replaced by a fluorine to increase bioavailability and biostability. The fate of fluorine released from fluorine-containing drugs is not well investigated. The aim of this study was to examine possible fluorination of proteins in rat liver and brain after administration of the fluorinated drug cinacalcet. We assigned 18 Wistar rats to a control group (n = 6) and a group treated with cinacalcet (2 mg kg−1/body weight, 5 days/week), divided into 7 day (n = 6) and 21 day (n = 6) treatment subgroups. Fluorinated proteins were identified using a free proteomics approach; chromatographic separation and analysis by high-resolution mass spectrometry; peptide/protein identification using the Mascot search algorithm; manual verification of an experimentally generated MS/MS spectrum with the theoretical MS/MS spectrum of identified fluorinated peptides. Three fluorinated proteins (spectrin beta chain; carbamoyl-phosphate synthase [ammonia], mitochondrial; 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1) were identified in the liver and four (spectrin beta chain, dihydropyrimidinase-related protein 4, prominin-2, dihydropyrimidinase-related protein 4) in the brain tissue after 21 days of cinacalcet treatment, but not in the control group. Introduction of fluorine into an organism by administration of fluorinated drugs results in tissue-specific fluorination of proteins.
Molecular Imaging in Nanomedical Research
Authors: Malatesta Manuela
Published: April 2022

For years, nanomedical research has represented a challenge and an opportunity in terms of imaging techniques [...]
Differential Nanoscale Topography Dedicates Osteocyte-Manipulated Osteogenesis via Regulation of the TGF-β Signaling Pathway
Authors: Cui Jingyuan , Yang Yaru , Chen Peiru , Hang Ruiqiang , Xiao Yin , Liu Xueting , Zhang Lixin , Sun Hui , Bai Long
Published: April 2022

Osteocytes function as the master orchestrator of bone remodeling activity in the telophase of osseointegration. However, most contemporary studies focus on the manipulation of osteoblast and/or osteoclast functionality via implant surface engineering, which neglects the pivotal role of osteocytes in de novo bone formation. It is confirmative that osteocyte processes extend directly to the implant surface, but whether the surface physicochemical properties can affect the functionality of osteocytes and determine the fate of the osseointegration in the final remodeling stage remains to be determined. Titania nanotube arrays (NTAs) with distinct diameters were fabricated to investigate the relationship between the nanoscale topography and the functionality of osteocytes. In vitro results pinpointed that NTA with a diameter of 15 nm (NTA-15) significantly promote osteogenesis of osteocytes via the enhancement of spreading, proliferation, and mineralization. The osteocyte transcriptome of each group further revealed that the TGF-β signaling pathway plays a pivotal role in osteocyte-mediated osteogenesis. The in vivo study definitely mirrored the aforementioned results, that NTA-15 significantly promotes bone formation around the implant. Consequently, nanoscaled topography-induced osteocyte functionality is important in late osseointegration. This suggests that surface designs targeting osteocytes may, therefore, be a potential approach to solving the aseptic loosening of the implant, and thus strengthen osseointegration.
The Role of Propranolol as a Repurposed Drug in Rare Vascular Diseases
Authors: Cuesta Angel M. , Gallardo-Vara Eunate , Casado-Vela Juan , Recio-Poveda Lucía , Botella Luisa-María , Albiñana Virginia
Published: April 2022

Rare Diseases (RD) are defined by their prevalence in less than 5 in 10,000 of the general population. Considered individually, each RD may seem insignificant, but together they add up to more than 7000 different diseases. Research in RD is not attractive for pharmaceutical companies since it is unlikely to recover development costs for medicines aimed to small numbers of patients. Since most of these diseases are life threatening, this fact underscores the urgent need for treatments. Drug repurposing consists of identifying new uses for approved drugs outside the scope of the original medical indication. It is an alternative option in drug development and represents a viable and risk-managed strategy to develop for RDs. In 2008, the “off label” therapeutic benefits of propranolol were described in the benign tumor Infantile Hemangioma. Propranolol, initially prescribed for high blood pressure, irregular heart rate, essential tremor, and anxiety, has, in the last decade, shown increasing evidence of its antiangiogenic, pro-apoptotic, vasoconstrictor and anti-inflammatory properties in different RDs, including vascular or oncological pathologies. This review highlights the finished and ongoing trials in which propranolol has arisen as a good repurposing drug for improving the health condition in RDs.
TaKLU Plays as a Time Regulator of Leaf Growth via Auxin Signaling
Authors: Zhou Mengdie , Peng Haixia , Wu Linnan , Li Mengyao , Guo Lijian , Chen Haichao , Wu Baowei , Liu Xiangli , Zhao Huixian , Li Wenqiang , Ma Meng
Published: April 2022

The growth of leaves is subject to strict time regulation. Several genes influencing leaf growth have been identified, but little is known about how genes regulate the orderly initiation and growth of leaves. Here, we demonstrate that TaKLU/TaCYP78A5 contributes to a time regulation mechanism in leaves from initiation to expansion. TaKLU encodes the cytochrome P450 CYP78A5, and its homolog AtKLU has been described whose deletion is detrimental to organ growth. Our results show that TaKLU overexpression increases leaf size and biomass by altering the time of leaf initiation and expansion. TaKLU-overexpressing plants have larger leaves with more cells. Further dynamic observations indicate that enlarged wheat leaves have experienced a longer expansion time. Different from AtKLU inactivation increases leaf number and initiation rates, TaKLU overexpression only smooths the fluctuations of leaf initiation rates by adjusting the initiation time of local leaves, without affecting the overall leaf number and initiation rates. In addition, complementary analyses suggest TaKLU is functionally conserved with AtKLU in controlling the leaf initiation and size and may involve auxin accumulation. Our results provide a new insight into the time regulation mechanisms of leaf growth in wheat.
DspA/E-Triggered Non-Host Resistance against E. amylovora Depends on the Arabidopsis GLYCOLATE OXIDASE 2 Gene
Authors: Launay Alban , Jolivet Sylvie , Clément Gilles , Zarattini Marco , Dellero Younes , Le Hir Rozenn , Jossier Mathieu , Hodges Michael , Expert Dominique , Fagard Mathilde
Published: April 2022

DspA/E is a type three effector injected by the pathogenic bacterium Erwinia amylovora inside plant cells. In non-host Arabidopsis thaliana, DspA/E inhibits seed germination, root growth, de novo protein synthesis and triggers localized cell death. To better understand the mechanisms involved, we performed EMS mutagenesis on a transgenic line, 13-1-2, containing an inducible dspA/E gene. We identified three suppressor mutants, two of which belonged to the same complementation group. Both were resistant to the toxic effects of DspA/E. Metabolome analysis showed that the 13-1-2 line was depleted in metabolites of the TCA cycle and accumulated metabolites associated with cell death and defense. TCA cycle and cell-death associated metabolite levels were respectively increased and reduced in both suppressor mutants compared to the 13-1-2 line. Whole genome sequencing indicated that both suppressor mutants displayed missense mutations in conserved residues of Glycolate oxidase 2 (GOX2), a photorespiratory enzyme that we confirmed to be localized in the peroxisome. Leaf GOX activity increased in leaves infected with E. amylovora in a DspA/E-dependent manner. Moreover, the gox2-2 KO mutant was more sensitive to E. amylovora infection and displayed reduced JA-signaling. Our results point to a role for glycolate oxidase in type II non-host resistance and to the importance of central metabolic functions in controlling growth/defense balance.
Identification of CDPK Gene Family in Solanum habrochaites and Its Function Analysis under Stress
Authors: Li Yingying , Zhang Haixin , Liang Sibo , Chen Xiuling , Liu Jiayin , Zhang Yao , Wang Aoxue
Published: April 2022

Tomato is an important vegetable crop. In the process of tomato production, it will encounter abiotic stress, such as low temperature, drought, and high salt, and biotic stress, such as pathogen infection, which will seriously affect the yield of tomato. Calcium-dependent protein kinase (CDPK) is a class of major calcium signal receptor which has an important regulatory effect on the perception and decoding of calcium signals. CDPK plays a key role in many aspects of plant growth, such as the elongation of pollen tubes, plant growth, and response to biotic and abiotic stress. While some studies have concentrated on Arabidopsis and pepper, Solanum habrochaites is a wild species relative of cultivated tomato and there is no report on CDPK in Solanum habrochaites to date. Using tomato genomic data, this study identified 33 members of the CDPK gene family. Evolutionary analysis divides family members into four Asian groups, of which the CDPK family members have 11 gene replication pairs. Subcellular location analysis showed that most proteins were predicted to be located in the cytoplasm, and less protein existed on the cell membrane. Not all CDPK family members have a transmembrane domain. Cis regulatory elements relating to light, hormones, and drought stress are overrepresented in the promoter region of the CDPK genes in Solanum habrochaites. The expression levels of each gene under biotic stress and abiotic stress were quantified by qRT-PCR. The results showed that members of the CDPK family in Solanum habrochaites respond to different biotic and abiotic stresses. Among them, the expression of ShCDPK6 and ShCDPK26 genes change significantly. ShCDPK6 and ShCDPK26 genes were silenced using VIGS (virus-induced gene silencing), and the silenced plants illustrated reduced stress resistance to Botrytis cinerea, cold, and drought stress. The results of this study will provide a basis for the in-depth study of the CDPK gene family in Solanum habrochaites, laying the foundation for further analysis of the function of the gene family.
Combination of Sanguisorbigenin and Conventional Antibiotic Therapy for Methicillin-Resistant Staphylococcus aureus: Inhibition of Biofilm Formation and Alteration of Cell Membrane Permeability
Authors: Wang Shu , Liu Xiang-Qian , Kang Ok-Hwa , Kwon Dong-Yeul
Published: April 2022

Methicillin-resistant Staphylococcus aureus (MRSA) infection is challenging to eradicate because of antibiotic resistance and biofilm formation. Novel antimicrobial agents and alternative therapies are urgently needed. This study aimed to evaluate the synergy of sanguisorbigenin (SGB) isolated from Sanguisorba officinalis L. with six conventional antibiotics to achieve broad-spectrum antibacterial action and prevent the development of resistance. A checkerboard dilution test and time-to-kill curve assay were used to determine the synergistic effect of SGB combined with antibiotics against MRSA. SGB showed significant synergy with antibiotics and reduced the minimum inhibitory concentration of antibiotics by 2–16-fold. Biofilm inhibition assay, quantitative RT-PCR, crystal violet absorption, and transmission electron microscopy were performed to evaluate the synergy mechanism. The results indicated that SGB could inhibit biofilm formation and alter cell membrane permeability in MRSA. In addition, SGB was found to exhibit quite low cytotoxicity and hemolysis. The discovery of the superiority of SGB suggests that SGB may be an antibiotic adjuvant for use in combination therapy and as a plant-derived antibacterial agent targeting biofilms.
Mannose Receptor-Mediated Carbon Nanotubes as an Antigen Delivery System to Enhance Immune Response Both In Vitro and In Vivo
Authors: Feng Haibo , Feng Yangyang , Lin Lang , Wu Daiyan , Liu Qianqian , Li Hangyu , Zhang Xinnan , Li Sheng , Tang Feng , Liu Ziwei , Zhang Linzi
Published: April 2022

Carbon nanotubes (CNTs) are carbon allotropes consisting of one, two, or more concentric rolled graphene layers. These can intrinsically regulate immunity by activating the innate immune system. Mannose receptors (MR), a subgroup of the C-type lectin superfamily, are abundantly expressed on macrophages and dendritic cells. These play a crucial role in identifying pathogens, presenting antigens, and maintaining internal environmental stability. Utilizing the specific recognition between mannose and antigen-presenting cells (APC) surface mannose receptors, the antigen-carrying capacity of mannose-modified CNTs can be improved. Accordingly, here, we synthesized the mannose-modified carbon nanotubes (M-MWCNT) and evaluated them as an antigen delivery system through a series of in vitro and in vivo experiments. In vitro, M-MWCNT carrying large amounts of OVA were rapidly phagocytized by macrophages and promoted macrophage proliferation to facilitate cytokines (IL-1β, IL-6) secretion. In vivo, in mice, M-MWCNT induced the maturation of dendritic cells and increased the levels of antigen-specific antibodies (IgG, IgG1, IgG2a, IgG2b), and cytokines (IFN-γ, IL-6). Taken together, M-MWCNT could induce both humoral and cellular immune responses and thereby can be utilized as an efficient antigen-targeted delivery system.
Synthesis, Characterization and Nanoformulation of Novel Sulfonamide-1,2,3-triazole Molecular Conjugates as Potent Antiparasitic Agents
Authors: Aljohani Faizah S. , Rezki Nadjet , Aouad Mohamed R. , Elwakil Bassma H. , Hagar Mohamed , Sheta Eman , Hussein Mogahed Nermine Mogahed Fawzy , Bardaweel Sanaa K. , Hagras Nancy Abd-elkader
Published: April 2022

Toxoplasma gondii (T. gondii) is a highly prevalent parasite that has no gold standard treatment due to the poor action or the numerous side effects. Focused sulfonamide-1,2,3-triazole hybrids 3a–c were wisely designed and synthesized via copper catalyzed 1,3-dipolar cycloaddition approach between prop-2-yn-1-alcohol 1 and sulfa drug azides 2a–c. The newly synthesized click products were fully characterized using different spectroscopic experiments and were loaded onto chitosan nanoparticles to form novel nanoformulations for further anti-Toxoplasma investigation. The current study proved the anti-Toxoplasma effectiveness of all examined compounds in experimentally infected mice. Relative to sulfadiazine, the synthesized sulfonamide-1,2,3-triazole (3c) nanoformulae demonstrated the most promising result for toxoplasmosis treatment as it resulted in 100% survival, 100% parasite reduction along with the remarkable histopathological improvement in all the studied organs.
Quadruped Gait and Regulation of Apoptotic Factors in Tibiofemoral Joints following Intra-Articular rhPRG4 Injection in Prg4 Null Mice
Authors: Yang Daniel S. , Dickerson Edward E. , Zhang Ling X. , Richendrfer Holly , Karamchedu Padmini N. , Badger Gary J. , Schmidt Tannin A. , Fredericks Alger M. , Elsaid Khaled A. , Jay Gregory D.
Published: April 2022

Camptodactyly-arthropathy-coxa vara-pericarditis (CACP) syndrome leads to diarthrodial joint arthropathy and is caused by the absence of lubricin (proteoglycan 4—PRG4), a surface-active mucinous glycoprotein responsible for lubricating articular cartilage. In this study, mice lacking the orthologous gene Prg4 served as a model that recapitulates the destructive arthrosis that involves biofouling of cartilage by serum proteins in lieu of Prg4. This study hypothesized that Prg4-deficient mice would demonstrate a quadruped gait change and decreased markers of mitochondrial dyscrasia, following intra-articular injection of both hindlimbs with recombinant human PRG4 (rhPRG4). Prg4−/− (N = 44) mice of both sexes were injected with rhPRG4 and gait alterations were studied at post-injection day 3 and 6, before joints were harvested for immunohistochemistry for caspase-3 activation. Increased stance and propulsion was shown at 3 days post-injection in male mice. There were significantly fewer caspase-3-positive chondrocytes in tibiofemoral cartilage from rhPRG4-injected mice. The mitochondrial gene Mt-tn, and myosin heavy (Myh7) and light chains (Myl2 and Myl3), known to play a cytoskeletal stabilizing role, were significantly upregulated in both sexes (RNA-Seq) following IA rhPRG4. Chondrocyte mitochondrial dyscrasias attributable to the arthrosis in CACP may be mitigated by IA rhPRG4. In a supporting in vitro crystal microbalance experiment, molecular fouling by albumin did not block the surface activity of rhPRG4.
Macrophages Cytokine Spp1 Increases Growth of Prostate Intraepithelial Neoplasia to Promote Prostate Tumor Progression
Authors: Messex Justin K. , Byrd Crystal J. , Thomas Mikalah U. , Liou Geou-Yarh
Published: April 2022

Prostate cancer development and progression are associated with increased infiltrating macrophages. Prostate cancer is derived from prostatic intraepithelial neoplasia (PIN) lesions. However, the effects macrophages have on PIN progression remain unclear. Here, we showed that the recruited macrophages adjacent to PIN expressed M2 macrophage markers. In addition, high levels of Spp1 transcripts, also known as osteopontin, were identified in these macrophages. Extraneously added Spp1 accelerated PIN cell proliferation through activation of Akt and JNK in a 3D culture setting. We also showed that PIN cells expressed CD44, integrin αv, integrin β1, and integrin β3, all of which have been previously reported as receptors for Spp1. Finally, blockade of Akt and JNK activation through their specific inhibitor completely abolished macrophage Spp1-induced cell proliferation of PIN. Hence, our data revealed Spp1 as another macrophage cytokine/growth factor and its mediated mechanism to upregulate PIN cell growth, thus promoting prostate cancer development.
Coded Caching for Combination Networks with Multiaccess
Authors: Huang Leitang , Wang Jinyu , Cheng Minquan , Deng Qingyong , Zhong Bineng
Published: April 2022

In a traditional (H,r) combination network, each user connects to a unique set of r relays. However, few research efforts have considered the (H,r,u) multiaccess combination network problem wherein each unique set of r relays is connected by u users. In this paper, we focus on designing coded caching schemes for a (H,r,u) multiaccess combination network. By directly applying the well-known coding method (proposed by Zewail and Yener) for a (H,r) combination network, a coded caching scheme (called ZY scheme) for (H,r,u) multiaccess combination network is obtained. However, its subpacketization has an exponential order with the number of users which leads to high implementation complexity. In order to reduce subpacketization, a direct construction of a coded caching scheme (called the direct scheme) for (H,r,u) multiaccess combination network is proposed by means of combinational design theory, where the parameter u must be a combinatorial number. For the arbitrary parameter u, the hybrid construction of a coded caching scheme (called the hybrid scheme) for the (H,r,u) multiaccess combination network is proposed based on the direct scheme. Theoretical and numerical analysis shows that the direct scheme and the hybrid scheme have a smaller transmission load for each relay compared with the naive scheme (which is obtained by repeatedly applying the coded caching scheme for a traditional (H,r) combination network by u times) and have much lower subpacketization compared with the ZY scheme.
Coupling Coordination of “Urban Rail Transit—Social Economy” Composite System
Authors: Wang Zhiheng , Li Xiaozhao , Bobylev Nikolai , Li Shuchen , Liu Jiangfeng
Published: April 2022

The harmonious development of urban rail transit, underground space engineering, and social economy is the key to regional sustainable development. Based on synergetic theory, this paper constructs the coupling coordination evaluation system of “rail transport-social economy” composite system at the scale of a city cluster. With this system, the coupling and coordinated development pattern and characteristics of “rail transit-social economy” in the Yangtze River Delta city cluster from 2002 to 2020 were analyzed. The paper makes a horizontal comparison with the Beijing-Tianjin-Hebei city cluster and the Pearl River Delta city cluster, as well as analyzes the differences in development and existing problems, and puts forward policy suggestions for rail and urban underground space development. The results show that: (1) The rail transit of 11 cities in the Yangtze River Delta shows a “step by step” development pattern. That is “national central city, provincial city, second-tier city, third-tier city, etc.”, accompanied by periodic changes of coupling and coordination degree. In addition, there is also the phenomenon of unbalanced development within the region; (2) From 2002 to 2020, the development of rail transit in the three city clusters shows a situation of “the overall supply is lacking and lags behind the social economy for a long time”. Among them, the Pearl River Delta city cluster has the most serious lags. Multi-channel financing, speeding up the construction of the rail transit scale according to local conditions, and improving operating efficiency are considered to be the keys to solve this problem; (3) In general, the coupling between rail transit and the social economy in the Yangtze River Delta city cluster is better than that in the Beijing-Tianjin-Hebei city cluster and the Pearl River Delta city cluster, but the coordination is at a slight disadvantage.
HLHS: Power of the Chick Model
Authors: Sedmera David
Published: April 2022

Background: Hypoplastic left heart syndrome (HLHS) is a rare but deadly form of human congenital heart disease, most likely of diverse etiologies. Hemodynamic alterations such as those resulting from premature foramen ovale closure or aortic stenosis are among the possible pathways. Methods: The information gained from studies performed in the chick model of HLHS is reviewed. Altered hemodynamics leads to a decrease in myocyte proliferation causing hypoplasia of the left heart structures and their functional changes. Conclusions: Although the chick phenocopy of HLHS caused by left atrial ligation is certainly not representative of all the possible etiologies, it provides many useful hints regarding the plasticity of the genetically normal developing myocardium under altered hemodynamic loading leading to the HLHS phenotype, and even suggestions on some potential strategies for prenatal repair.
Length of Hospital Stay after Total Knee Arthroplasty: A Correlation Study on 1200 Patients
Authors: Papalia Rocco , Torre Guglielmo , Alifano Anna Maria , Albo Erika , Papalia Giuseppe Francesco , Bravi Marco , De Vincentis Antonio , Zappalà Emanuele , Zampogna Biagio , Denaro Vincenzo
Published: April 2022

In countries with a high average population age, total knee arthroplasty is still carried out in an inpatient setting. The roadmap to performing major surgery on an outpatient basis passes through the understanding of those perioperative features that correlate with higher lengths of hospital stay (LOS). A retrospective database of 1200 patients was reviewed for retrieving preoperative and perioperative factors including anthropometric and demographic data, comorbidities, preoperative laboratory assessment, and surgical time. Considering the LOS as a discrete series, data were analyzed by means of logistic regression with multiple univariate and multivariate models. The results showed a median length of hospital stay of 3 (IQR 3, 4) days. According to multiple univariate analysis, arterial hypertension (p = 0.008), diabetes mellitus (p = 0.028), CCI score (p < 0.001), ASA score (p = 0.006), surgical time (p < 0.001) and intraoperative blood loss (p < 0.001) were significantly associated with the duration of hospital stay in days. Moreover, preoperative hemoglobin value was inversely correlated to the LOS (p = 0.008). Multivariate analysis showed a significant correlation between LOS and surgical time and intraoperative blood loss. Many factors influence the permanence of the inpatient and acting on those variables, by stabilizing comorbidities and optimizing laboratory values, may reduce the overall healthcare burden.
Neuronal Pre- and Postconditioning via Toll-like Receptor 3 Agonist or Extracorporeal Shock Wave Therapy as New Treatment Strategies for Spinal Cord Ischemia: An In Vitro Study
Authors: Lobenwein Daniela , Huber Rosalie , Kerbler Lars , Gratl Alexandra , Wipper Sabine , Gollmann-Tepeköylü Can , Holfeld Johannes
Published: April 2022

Spinal cord ischemia (SCI) is a devastating and unpredictable complication of thoracoabdominal aortic repair. Postischemic Toll-like receptor 3 (TLR3) activation through either direct agonists or shock wave therapy (SWT) has been previously shown to ameliorate damage in SCI models. Whether the same applies for pre- or postconditioning remains unclear. In a model of cultured SHSY-5Y cells, preconditioning with either poly(I:C), a TLR3 agonist, or SWT was performed before induction of hypoxia, whereas postconditioning treatment was performed after termination of hypoxia. We measured cytokine expression via RT-PCR and utilized Western blot analysis for the analysis of signaling and apoptosis. TLR3 activation via poly(I:C) significantly reduced apoptotic markers in both pre- and postconditioning, the former yielding more favorable results through an additional suppression of TLR4 and its downstream signaling. On the contrary, SWT showed slightly more favorable effects in the setting of postconditioning with significantly reduced markers of apoptosis. Pre- and post-ischemic direct TLR3 activation as well as post-ischemic SWT can decrease apoptosis and proinflammatory cytokine expression significantly in vitro and might therefore pose possible new treatment strategies for ischemic spinal cord injury.