bluuebunny/crossref_metadata_2025 huggingface dataset:
Hepatoprotective and Fat-Accumulation-Reductive Effects of Curcumin on Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD)
Authors: Sabini Jasmine Harumi , Timotius Kris Herawan
Published: February 2025

Fat accumulation is the hallmark of metabolic dysfunction-associated steatotic liver disease (MASLD). Given the intimidating nature of its treatment, curcumin (CUR) emerges as a potential therapeutic agent due to its proven effectiveness in managing MASLD. This review aimed to evaluate previous reports on the hepatoprotective and fat-accumulation-reductive effects of CUR administration in preventing or treating MASLD. CUR administration can modulate serum liver enzymes and lipid profiles. The fat accumulation of MASLD is the primary cause of oxidative stress and inflammation. By reducing fat accumulation, CUR may attenuate the inflammation and oxidative stress in MASLD. In addition, CUR has been proven to restore the dysfunctional cellular energy metabolism capacity and attenuate fibrogenesis (antifibrotic agent). Their hepatoprotective effects are associated with fat accumulation in MASLD. Lipid metabolism (lipogenesis, lipolysis, and lipophagy) is correlated with their hepatoprotective effects. CUR has prophylactic and therapeutic effects, particularly in early-stage MASLD, primarily when it is used as a fat reducer. It can be considered an excellent natural therapeutic drug for MASLD because it protects the liver and attenuates fat accumulation, especially in the early stage of MASLD development.
Editorial for Special Issue: “Molecular Mechanisms Underlying Fatty Liver Disease: From Pathogenesis to Treatment”
Authors: Dong Haibo
Published: February 2025

Metabolic-dysfunction-associated fatty liver disease (MAFLD) and alcohol-associated liver disease (ALD) represent two of the most prevalent chronic liver diseases globally, collectively affecting hundreds of millions of individuals [...]
Lycobetaine Has Therapeutic Efficacy in Lung Squamous Cell Carcinoma by Targeting USP32 to Trigger Ferroptosis
Authors: Xing Shangping , Chai Hua , Chen Zhenlong , Deng Shuye , Nong Feifei
Published: February 2025

Ubiquitin-specific protease 32 (USP32), a deubiquitylating enzyme that controls the ubiquitin process, is overexpressed in multiple cancers and serves as a promising therapeutic target for cancer therapy. Drugs targeting ferroptosis have exhibited promising anticancer activity. Lycobetaine (LBT), a natural alkaloid, holds promise against various cancers, yet its specific targets and anticancer mechanisms remain unclear. In this study, we show that LBT induced ferroptosis in lung squamous cell carcinoma (LUSC) cells, accompanied by glutathione depletion and the accumulation of lipid peroxidation, malondialdehyde, and ferrous iron. Mechanistically, drug affinity responsive target stability-based mass spectrometry analysis, molecular dynamics simulations, and a cellular thermal shift assay confirmed that USP32 is a potential target of LBT in LUSC cells. Moreover, a strong interaction between USP32 and nuclear factor erythroid 2-related factor 2 (NRF2) was found via immunoprecipitation–mass spectrometry and co-immunoprecipitation. In addition, the ubiquitination assay results demonstrated that LBT treatment significantly increased NRF2 ubiquitination and degradation by targeting USP32. Importantly, USP32 overexpression effectively attenuated the effects of LBT on proliferation and ferroptosis in LUSC cells. In orthotopic LUSC xenografts, the administration of LBT significantly inhibited tumor growth and metastasis and induced ferroptosis by targeting the USP32–NRF2 signaling axis. Taken together, these data suggest that LBT exerts its anticancer effects by inhibiting USP32-mediated NRF2 deubiquitination to induce ferroptosis and that LBT may serve as a prospective USP32-targeting agent for LUSC treatment.
The Proliferation of Chang Liver Cells After Simulated Microgravity Induction
Authors: Hoang Huy Nghia Quang , Ho Chi Nguyen Quynh , Dang Loan Thi Tung , Phan Nhan Lu Chinh , Doan Chung Chinh , Nguyen Han Thai Minh , Le Cuong Phan Minh , Hoang Son Nghia , Le Long Thanh
Published: February 2025

This study aimed to assess the recovery capability of Chang liver cells (CCL-13) following simulated microgravity (SMG) induction. CCL-13 cells were cultured under SMG conditions for 72 h, and control group cells were cultured under 1G conditions for an identical duration. Cells from the SMG and control groups were further cultured under 1G conditions and assessed after 24 h and 72 h intervals in the gravity recovery experiment. The WST1 results indicated that CCL-13 proliferation was more evident in the control group than in the SMG group after both the 24 h and 72 h intervals. The control group had a lower percentage of CCL-13 cells in the G0/G1 phase compared with the SMG group at both time points, and it exhibited a higher total percentage of cells in the S and G2/M phases. The control group exhibited elevated levels of cell-cycle-related proteins, including cyclin A, cyclin D, and cdk6, compared with the SMG group. The flow cytometry results revealed that the apoptotic rate in the control group was significantly lower than that in the SMG group at both the 24 h and 72 h time points. However, the apoptotic percentage in the SMG group at the 72-h mark was significantly lower than that at the 24-h mark. SMG reduces the viability and proliferation ability of CCL-13 cells. After a period of recovery and adaptation to normal gravity conditions (1G), the CCL-13 cells in the SMG group showed better signs of recovery after 72 h than after 24 h.
Influence of La/Al Doping via Magnetron Sputtering on the Mechanical and Tribological Properties of TiN Coatings
Authors: Chang Weihang , Zhang Hongfeng , Tian Changling , Xue Yujun , Liu Gang
Published: February 2025

Adding alloying elements to binary nitrides enables the design of hard and tough coatings. To improve the mechanical and tribological performances of TiN-based coatings, La atoms were added to TiAlN coatings to form TiAlLaN coatings. Magnetron sputtering was conducted to prepare the TiAlLaN coatings. Thereafter, scanning electron microscopy (SEM), x-ray diffraction (XRD), nano-indentation, and a tribometer were utilized to test their microstructure, phases, and mechanical and tribological performances. Next, this study analyzed how lanthanum affected the microstructure and tribological performances of the TiAlLaN coatings. Incorporating La atoms in TiAlN coatings reduced the crystallite size and enhanced the coating toughness and hardness. The hardness H and elastic modulus E of the TiAlLaN coatings first increased and then decreased with the increase in La. Meanwhile, the coatings had improved wear and friction properties. The increased H/E and H3/E2 levels, which have been considered to reflect the hard coating’s toughness, were acquired based on the TiAlLaN coating, possessing enhanced hardness (19.8 GPa). The coefficient of friction and the wear rates of the coatings reduced and then increased with the increase in La. The TiAlLaN coating with 1.4 % of lanthanum had the lowest friction coefficient and wear rate of around 0.383 and 1.59 × 10−8 mm3/N·m, respectively, corresponding to a higher H/E (~0.086) and H3/E2 (~0.147 GPa). Adding an appropriate amount of La can substantially enhance the TiAlN coating’s tribological and mechanical properties. The TiAlLaN coating with remarkable characteristics may be applied to a steel substrate.
Nanosized Being of Ionic Surfactant Micelles: An Advanced View on Micellization Process
Authors: Zueva Olga S. , Kazantseva Mariia A. , Zuev Yuriy F.
Published: February 2025

An advanced model of ionic surfactant micellization has been developed. The structural and kinetic properties of micelles were analyzed in parallel from a universally accepted point of view and taking into account the principles of quantum mechanics, the phenomenon of ion pairing in the Stern layer, the symmetry considerations, and the chaos theory. It was shown that a micelle can be considered as a layered fullerene-like structure with a cavity in its center, possessing the solid-like properties of micelles in radial directions and the liquid-like properties in the perpendicular ones, allowing for water penetration between the surfactant head group and nearby methylene groups. The dimensions of the minimal fullerene-like structure formed by the terminal hydrogen atoms of surfactant methyl groups around the central cavity, unable to be occupied by surfactant tail fragments, were estimated. It was indicated that permanently occurring surfactant self-organization/disintegration needs a probabilistic description and revision of processes occurring in micellar systems built by ionic surfactants. It was noted that the probabilistic approach alters the mechanism of colloidal dissolution of hydrocarbon compounds and their solubilization by micelles. The advanced model proposes the same macroscopic properties of micelles as the classical one but modifies the structural characteristics of micelles on the nanoscale.
Breast Cancer and Tumor Microenvironment: The Crucial Role of Immune Cells
Authors: Moura Tânia , Laranjeira Paula , Caramelo Olga , Gil Ana M. , Paiva Artur
Published: February 2025

Breast cancer is the most common type of cancer in women and the second leading cause of death by cancer. Despite recent advances, the mortality rate remains high, underlining the need to develop new therapeutic approaches. The complex interaction between cancer cells and the tumor microenvironment (TME) is crucial in determining tumor progression, therapy response, and patient prognosis. Understanding the role of immune cells in carcinogenesis and tumor progression can help improve targeted therapeutic options, increasing the likelihood of a favorable prognosis. Therefore, this review aims to critically analyze the complex interaction between tumor cells and immune cells, emphasizing the clinical and therapeutic implications. Additionally, we explore advances in immunotherapies, with a focus on immune checkpoint inhibitors.
Development and Dynamic Numerical Evaluation of a Lightweight Sports Helmet Using Topology Optimization and Advanced Architected Materials
Authors: Kladovasilakis Nikolaos , Tsongas Konstantinos , Pechlivani Eleftheria Maria , Tzetzis Dimitrios
Published: February 2025

Sports activities often carry a high risk of injury, varying in severity, making the use of protective equipment, such as helmets and kneecaps, essential in many cases. Among all potential injuries, head injuries are the most crucial due to their severity. Hence, in the last decades, the scientific interest has been focused on establishing head injury criteria and improving the helmet design with the ultimate goal of the reduction in injury probability and increasing the athlete’s performance. In this context, the current study aims to develop a lightweight sports helmet with increased safety performance, utilizing topology optimization processes and advanced architected materials. In detail, the design of a conventional helmet was developed and modified applying in specific regions advanced architected materials, such as triply periodic minimal surfaces (TPMS) and hybrid structures, with functionally graded configurations to produce sandwich-like structures capable of absorbing mechanical energy from impacts. The developed helmet’s designs were numerically evaluated through dynamic finite element analyses (FEA), simulating the helmet’s impact on a wall with a specific velocity. Through these analyses, the plastic deformation of the designed helmets was observed, coupled with the stress concentration contours. Furthermore, the results of FEAs were utilized in order to calculate the values of the head injury criterion (HIC). Finally, the developed topologically optimized helmet design incorporating the hybrid lattice structure revealed increased energy absorption, reaching a HIC of 1618, improved by around 14% compared to the conventional design configuration.
Advances in Novel Diagnostic Techniques for Alveolar Echinococcosis
Authors: Liu Huanhuan , Xie Yijia , An Xiaoyu , Xu Dazhuang , Cai Shundong , Chu Chengchao , Liu Gang
Published: February 2025

Alveolar echinococcosis (AE), caused by the larval stage of the tapeworm Echinococcus multilocularis, is a serious parasitic disease that presents significant health risks and challenges for both patients and healthcare systems. Accurate and timely diagnosis is essential for effective management and improved patient outcomes. This review summarizes the latest diagnostic methods for AE, focusing on serological tests and imaging techniques such as ultrasonography (US), computed tomography (CT), magnetic resonance imaging (MRI), and positron emission tomography/computed tomography (PET/CT). Each imaging modality has its strengths and limitations in detecting and characterizing AE lesions, such as their location, size, and invasiveness. US is often the first-line method due to its non-invasiveness and cost-effectiveness, but it may have limitations in assessing complex lesions. CT provides detailed anatomical information and is particularly useful for assessing bone involvement and calcification. MRI, with its excellent soft tissue contrast, is superior for delineating the extent of AE lesions and their relationship to adjacent structures. PET/CT combines functional and morphological imaging to provide insights into the metabolic activity of lesions, which is valuable for monitoring treatment response and detecting recurrence. Overall, this review emphasizes the importance of a multifaceted diagnostic approach that combines serological and imaging techniques for accurate and early AE diagnosis, which is crucial for effective management and improved patient outcomes.
Mixture of Expert-Based SoftMax-Weighted Box Fusion for Robust Lesion Detection in Ultrasound Imaging
Authors: Rhyou Se-Yeol , Yu Minyung , Yoo Jae-Chern
Published: February 2025

Background/Objectives: Ultrasound (US) imaging plays a crucial role in the early detection and treatment of hepatocellular carcinoma (HCC). However, challenges such as speckle noise, low contrast, and diverse lesion morphology hinder its diagnostic accuracy. Methods: To address these issues, we propose CSM-FusionNet, a novel framework that integrates clustering, SoftMax-weighted Box Fusion (SM-WBF), and padding. Using raw US images from a leading hospital, Samsung Medical Center (SMC), we applied intensity adjustment, adaptive histogram equalization, low-pass, and high-pass filters to reduce noise and enhance resolution. Data augmentation generated ten images per one raw US image, allowing the training of 10 YOLOv8 networks. The mAP@0.5 of each network was used as SoftMax-derived weights in SM-WBF. Threshold-lowered bounding boxes were clustered using Density-Based Spatial Clustering of Applications with Noise (DBSCAN), and outliers were managed within clusters. SM-WBF reduced redundant boxes, and padding enriched features, improving classification accuracy. Results: The accuracy improved from 82.48% to 97.58% with sensitivity reaching 100%. The framework increased lesion detection accuracy from 56.11% to 95.56% after clustering and SM-WBF. Conclusions: CSM-FusionNet demonstrates the potential to significantly improve diagnostic reliability in US-based lesion detection, aiding precise clinical decision-making.
The Impact of Clinical Sample Transportation by Unmanned Aerial Systems on the Results of Laboratory Tests
Authors: Shapira Maanit , Cohen Ben , Friemann Sarit , Tal Yana , Teper Zila , Dudkiewicz Mickey , Portuguese Shirley , Na’amnih Wasef , Shriki Dikla Dahan
Published: February 2025

Transport by unmanned aerial systems (UASs) (e.g., drones) could save time and personnel. Our study aimed to assess the effect of drone transportation on the clinical laboratory results of biological samples by examining its impact on pre-analytical and analytical processes. We performed a cross-sectional study of healthy volunteers from Sha’ar Menashe Mental Health Center between July and November 2022. Blood and urine samples were transferred to the central laboratory at Hillel Yaffe Medical Center. Overall, 40 healthcare workers aged 21–67 years (57.5% females) with a mean age of 45.8 (SD = 11.3) years from Sha’ar Menashe Mental Health Center were recruited in the study. There were no significant differences between transportation modes in the complete blood count levels. We found a significant difference between the transportation modes for GGT (p = 0.01) and PT (p = 0.04), despite the very similar mean results of these tests. In Bland–Altman plots, GGT and PT samples fell within the 95% limits of agreement and were indicated as not clinically relevant; however, glucose and LDH did not meet the 95% acceptance criterion and showed a potential clinical effect. There was full agreement between the two types of transportation for urine glucose, nitrites, and urine cultures. UAS transport is an appropriate method for maintaining the quality of most routine clinical laboratory specimens, similar to the routine procedure of using a vehicle. For the 34 biochemistry, hematology, and coagulation assay parameters, only glucose and LDH did not meet the 95% acceptance criterion and showed a potential clinical effect.
Adapting Psychiatric Approaches to the Needs of Vulnerable Populations: A Qualitative Analysis
Authors: Besson Pascale , Gagné Lison , Bertulies-Esposito Bastian , Hudon Alexandre
Published: February 2025

Marginalized populations face significant barriers to mental health care, such as stigma, poverty, and limited access to adapted services, with conventional psychiatric approaches often falling short. This study aimed to explore how psychiatric care can be adapted to better meet the needs of vulnerable populations. Data were collected from psychiatry residents, psychiatrists, and community organization staff during a course on vulnerable populations, using semi-structured discussions and analyzed through grounded theory with iterative coding. Seven main themes emerged: (1) barriers and needs of vulnerable populations, highlighting challenges like homelessness and stigma; (2) psychiatric interventions and flexible approaches, emphasizing tailored care; (3) collaboration with community organizations, focusing on partnerships to improve care access; (4) ethical approach and respect for rights, ensuring dignity in treatment; (5) specific populations and associated challenges, addressing the needs of groups like LGBTQ+ youth and migrants; (6) intervention and support models, such as proximity-based care and post-hospitalization follow-up; (7) innovation and evolution of practices, focusing on research and institutional adaptations. This study emphasizes the need for personalized, intersectoral care, recommending improved collaboration, flexible models, and greater clinical exposure, with future research exploring how psychiatric education can better prepare clinicians to work with marginalized groups.
Chaos in Fractional-Order Glucose–Insulin Models with Variable Derivatives: Insights from the Laplace–Adomian Decomposition Method and Generalized Euler Techniques
Authors: Saber Sayed , Solouma Emad , Alharb Rasmiyah A. , Alalyani Ahmad
Published: February 2025

This study investigates the complex dynamics and control mechanisms of fractional-order glucose–insulin regulatory systems, incorporating memory-dependent properties through fractional derivatives. Employing the Laplace–Adomian Decomposition Method (LADM) and the Generalized Euler Method (GEM), the research models glucose–insulin interactions with time-varying fractional orders to simulate long-term physiological processes. Key aspects include the derivation of Lyapunov exponents, bifurcation diagrams, and phase diagrams to explore system stability and chaotic behavior. A novel control strategy using simple linear controllers is introduced to stabilize chaotic oscillations. The effectiveness of this approach is validated through numerical simulations, where Lyapunov exponents are reduced from positive values (λ1=0.123) in the uncontrolled system to negative values (λ1=−0.045) post-control application, indicating successful stabilization. Additionally, bifurcation analysis demonstrates a shift from chaotic to periodic behavior when control is applied, and time-series plots confirm a significant reduction in glucose–insulin fluctuations. These findings underscore the importance of fractional calculus in accurately modeling nonlinear and memory-dependent glucose–insulin dynamics, paving the way for improved predictive models and therapeutic strategies. The proposed framework provides a foundation for personalized diabetes management, real-time glucose monitoring, and intelligent insulin delivery systems.
Cyclodextrin–Hydrogel Hybrids in Advanced Drug Delivery
Authors: Omidian Hossein , Akhzarmehr Arnavaz , Gill Erma J.
Published: February 2025

Cyclodextrin (CD)–hydrogel hybrids have emerged as versatile and multifunctional drug delivery systems, offering enhanced solubility, controlled drug release, and improved bioavailability. By combining the inclusion complexation properties of CDs with the swelling and retention capabilities of hydrogels, these hybrid systems overcome key challenges in conventional drug formulations. This review explores CD composition, hydrogel polymer selection, fabrication techniques, key drug release factors, and real-world therapeutic applications. Additionally, the latest advancements in stimuli-responsive hydrogels, nanogels, and microneedle-based drug delivery are discussed. While CD–hydrogel systems demonstrate significant potential, scalability, regulatory hurdles, and clinical translation remain key challenges. Future research should focus on smart hydrogels, improved drug loading strategies, and enhanced clinical validation to bridge the gap between laboratory innovations and commercial applications.
Targeting Optimal Bone Regions: Correlations Between Bone Density and DNA Quality in Small Skeletal Elements
Authors: Geršak Živa Miriam , Salapura Vladka , Podovšovnik Eva , Zupanič-Pajnič Irena
Published: February 2025

Background: Identifying the optimal bone regions for DNA analysis is critical, as DNA preservation and quality vary significantly across bone types and structures and is defined as intra-bone variability. This study aimed to evaluate the correlation between computed tomography (CT)-measured bone density and DNA preservation in small skeletal elements to identify optimal regions for DNA analysis. Methods: 137 bones from six skeletal elements excavated from a single burial site were analysed using Dual-Source CT (DSCT) to map compact and cancellous regions. DNA was extracted using a demineralisation method and quantified via real-time PCR to assess DNA quantity and degradation. Results: Among 461 bone regions analysed (137 bones; patellae, calcaneus, talus, the navicular bones, the cuboid bone, and the medial cuneiform bone), a significant difference in DNA quantity was observed only in the calcaneus, where the sulcus contained more DNA than the body. No significant differences in the degradation index were detected among bone segments or skeletal elements. Correlations between CT-measured bone density and DNA quantity or degradation index were region-specific. Higher bone density correlated positively with DNA quantity in compact regions of the calcaneus and talus. Regarding degradation, a positive correlation (higher bone density → higher degradation) was observed in the patella’s anterior surface, while a negative correlation (higher bone density → lower degradation) was found in the talus’s sulcus, the opposite side of the talar sulcus, the posterior calcaneal articular facet, and the cuboid’s tuberosity. No significant correlations were found in other bone segments. Conclusions: Our study identified small skeletal elements, particularly the patella and the navicular bone, as promising sources for DNA analysis. While bone density correlated with DNA preservation in some cases, the relationship was inconsistent. Our findings support the use of small bones in forensic and archaeological research and warrant further investigation.
miR395e from Manihot esculenta Decreases Expression of PD-L1 in Renal Cancer: A Preliminary Study
Authors: Bogusławska Joanna , Rakhmetullina Aizhan , Grzanka Małgorzata , Białas Alex , Rybicka Beata , Życka-Krzesińska Joanna , Molcan Tomasz , Zielenkiewicz Piotr , Pączek Leszek , Piekiełko-Witkowska Agnieszka
Published: February 2025

Background/Objectives: microRNAs are small non-coding RNAs that regulate gene expression by inducing mRNA degradation or inhibiting translation. A growing body of evidence suggests that miRNAs may be utilized as anti-cancer therapeutics by targeting expression of key genes involved in cancerous transformation and progression. Renal cell cancer (RCC) is the most common kidney malignancy. The most efficient RCC treatments involve blockers of immune checkpoints, including antibodies targeting PD-L1 (Programmed Death Ligand 1). Interestingly, recent studies revealed the cross-kingdom horizontal transfer of plant miRNAs into mammalian cells, contributing to the modulation of gene expression by food ingestion. Here, we hypothesized that PD-L1 expression may be modulated by miRNAs originating from edible plants. Methods: To verify this hypothesis, we performed bioinformatic analysis to identify mes-miR395e from Manihot esculenta (cassava) as a promising candidate miRNA that could target PD-L1. To verify PD-L1 regulation mediated by the predicted plant miRNA, synthetic mes-miR395 mimics were transfected into cell lines derived from RCC tumors, followed by evaluation of PD-L1 expression using qPCR and Western blot. Results: Transfection of mes-miR395e mimics into RCC-derived cell lines confirmed that this miRNA decreases expression of PD-L1 in RCC cells at both mRNA and protein levels. Conclusions: This preliminary study shows the promise of plant miRNA as potential adjuvants supporting RCC treatment.
Carrier-Based Application of RsPod1EGY Phage to Effective Control Potato Bacterial Wilt
Authors: Elhalag Kamel M. , Mazrou Yasser S. A. , Elzaawely Abdelnaser A. , Makhlouf Abeer H. , Elsaed Sherine A. , Maswada Hanafey F. , Xuan Tran Dang , El-Nagar Asmaa
Published: February 2025

Bacterial wilt, caused by Ralstonia solanacearum, poses a significant threat to Solanaceae crops, including potatoes. Traditionally, chemical bactericides have been the primary method of disease management. However, the excessive and repeated use of these chemicals has led to the emergence of resistant R. solanacearum strains and raised environmental and safety concerns. Therefore, the current study aimed to explore bacteriophages as a sustainable and eco-friendly alternative to chemical control. Specifically, we evaluated the efficacy of the bacteriophage RsPod1EGY as a biocontrol agent under greenhouse conditions to reduce disease severity and maintain potato productivity. To improve phage survival and activity, we tested six carrier types; compost, peat moss, clay soil, sandy soil, talc powder, and wheat bran. Our findings indicated that compost, talc powder, and wheat bran significantly prolonged the phage’s viability and activity, reducing disease severity by 90%, compared to 65% and 85% for peat moss and phage-alone treatments respectively. In contrast, pathogen control treatments resulted in complete plant mortality. Phage survival was enhanced in pathogen-infected soils, supporting its role in reducing R. solanacearum populations. Potato yield was preserved in compost and talc powder treatments comparable to pathogen-free controls. These results underscore the potential of integrating bacteriophage RsPod1EGY with suitable carriers for sustainable bacterial wilt management, highlighting the importance of delivery methods in agricultural applications.
Comprehensive Analysis of Influenza Viruses’ Trends in Italy: Insights from a Nationwide and Regional Perspective
Authors: Branda  Francesco , Petrosillo  Nicola , Yon  Dong Keon , Ciccozzi  Massimo , Scarpa  Fabio
Published: February 2025

Background. Influenza remains a significant public health issue, with seasonal trends varying across regions. This study provides a comprehensive analysis of influenza virus trends in Italy, leveraging epidemiological and virological data from the Istituto Superiore di Sanità (ISS). The primary objective is to assess influenza activity at both national and regional levels, highlighting variations in incidence rates and viral subtype circulation during the 2023/2024 season. Methods. We conducted a systematic approach to data collection, processing, and visualization, utilizing influenza surveillance data from ISS. Incidence rates, subtype distribution, and co-circulating respiratory viruses were analyzed to identify key trends. Results. Our findings reveal a significant increase in influenza cases during the 2023/2024 season, with incidence rates surpassing pre-pandemic levels. Notably, changes in the circulation of influenza A(H3N2) and influenza B were observed, alongside the presence of other respiratory viruses such as RSV and rhinovirus. Conclusions. This study underscores the importance of real-time surveillance, transparent data sharing, and advanced visualization tools in guiding public health responses. By integrating lessons from COVID-19, we highlight the necessity of standardized surveillance frameworks to enhance preparedness for future seasonal outbreaks and potential pandemics.
The Therapeutic Potential of Myo-Inositol in Managing Patients with Respiratory Diseases
Authors: Quecchia Cristina , Vianello Andrea
Published: February 2025

Respiratory diseases are major health concerns worldwide. Chronic respiratory diseases (CRDs) are the third leading cause of death worldwide and some of the most common are chronic obstructive pulmonary disease (COPD), asthma, occupational lung diseases, and pulmonary hypertension. Despite having different etiology and characteristics, these diseases share several features, such as a persistent inflammatory state, chronic oxidative stress, impaired mucociliary clearance, and increased alveolar surface tension. CRDs are not curable; however, various forms of treatment, that help restore airway patency and reduce shortness of breath, can improve daily life for people living with these conditions. In this regard myo-inositol may represent a valid therapeutic adjuvant approach due to its properties. Being a redox balancer, an inflammation modulator, and, most importantly, a component of pulmonary surfactant, it may improve lung function and counteract symptoms associated with respiratory diseases, as recently evidenced in patients with COPD, COVID-19, asthma, and bronchiectasis. The aim of this review is to evaluate the potential therapeutic role of myo-inositol supplementation in the management of patients with respiratory diseases.
Proactive Data Categorization for Privacy in DevPrivOps
Authors: Silva Catarina , Barraca João P. , Salvador Paulo
Published: February 2025

Assessing privacy within data-driven software is challenging due to its subjective nature and the diverse array of privacy-enhancing technologies. A simplistic personal/non-personal data classification fails to capture the nuances of data specifications and potential privacy vulnerabilities. Robust, privacy-focused data categorization is vital for a deeper understanding of data characteristics and the evaluation of potential privacy risks. We introduce a framework for Privacy-sensitive Data Categorization (PsDC), which accounts for data inference from multiple sources and behavioral analysis. Our approach uses a hierarchical, multi-tiered tree structure, encompassing direct data categorization, dynamic tags, and structural attributes. PsDC is a data-categorization model designed for integration with the DevPrivOps methodology and for use in privacy-quantification models. Our analysis demonstrates its applicability in network-management infrastructure, service and application deployment, and user-centered design interfaces. We illustrate how PsDC can be implemented in these scenarios to mitigate privacy risks. We also highlight the importance of proactively reducing privacy risks by ensuring that developers and users understand the privacy “value” of data.
Association of Ankle–Brachial Index with Quality of Life and Survival Outcomes in Hemodialysis Patients
Authors: Yoshida Norihito , Tanaka Tatsuki , Suzuki Yusuke , Takahashi Sadamu , Hitaka Mai , Ishii Shingo , Yamazaki Keisuke , Ohashi Yasushi
Published: February 2025

Background/Objectives: Ankle–brachial index (ABI) is frequently measured in hemodialysis patients due to their high cardiovascular risk, while its potential role as a screening tool for assessing overall physical function and health-related quality of life (QOL) remains unclear. This study aimed to evaluate the association of the ABI with QOL and survival in hemodialysis patients. Methods: This study included 346 hemodialysis patients, categorized into two groups based on their ABI (≤0.9 vs. >0.9). Clinical parameters, QOL (measured using SF-36 and KDQOL questionnaires), and survival outcomes were analyzed. Results: There were 66 (19.1%) patients with an ABI ≤ 0.9 in this study population. Patients with an ABI ≤ 0.9 exhibited significantly older ages, longer dialysis durations, higher prevalence of diabetes mellites and cardiovascular disease, elevated N-terminal pro-brain natriuretic peptide levels, and higher calcitriol use but lower phase angle, skeletal muscle mass index values, health-related QOL domains, and several kidney disease-specific QOL domains compared to those with an ABI > 0.9. Kaplan–Meier analysis revealed significantly higher cumulative mortality in the ABI ≤ 0.9 group (6.6 vs. 2.5 per 100 patient-years, p < 0.001). Conclusions: A low ABI is significantly associated with decreased QOL and higher mortality risk in hemodialysis patients. While traditionally used for PAD screening, the ABI may serve as a practical tool for predicting QOL decline and survival outcomes. Interestingly, the ABI was also linked to muscle attenuation and volume overload. ABI assessment could aid in early risk stratification and guide multidisciplinary interventions, including exercise programs, nutritional support, and cardiovascular risk management, to improve patient care and outcomes.
Potential Glycobiomarkers in Maternal Obesity and Gestational Diabetes During Human Pregnancy
Authors: Farkas Anna , Suranyi Andrea , Kolcsar Balint , Gyurkovits Zita , Kozinszky Zoltan , Vari Sandor G. , Guttman Andras
Published: February 2025

Introduction: Obesity is a rapidly growing common health problem worldwide that can lead to the development of gestational diabetes mellitus (GDM). However, GDM not only affects women with obesity but can also develop at any time, even after the OGTT test; therefore, an increasing number of complications related to GDM can be seen in both mothers and their children. It is necessary to discover biomarkers capable of indicating the development of GDM or complications during/after pregnancy. Since the N-glycosylation motif of human IgG has been described to change under many physiological and pathological conditions, it is a promising target for biomarker research. In our study, the effects of obesity and GDM were investigated on human serum IgG N-linked glycosylation patterns during human pregnancy. Materials and Methods: The study participants were categorized into four groups according to their body mass index (BMI) and GDM status: normal weight as control, obese (BMI > 30 kg/m2), normal weight with GDM, and obese with GDM. The released N-glycan components of IgG were separated with capillary electrophoresis and detected using a laser-induced fluorescence detector. Results: The result revealed several differences between the N-glycosylation patterns of the four study groups. Of this, 17 of the 20 identified structures differed significantly between the groups. The ratios of sialylated to non-sialylated structures were not changed significantly, but the core fucosylation level showed a significant decrease in the GDM and obese GDM groups compared to the control subjects. The lowest degree of core fucosylation was observed in the GDM group. Conclusions: The findings indicate that obesity in isolation does not have a significant impact on the IgG N-glycosylation pattern in pregnancy. Conversely, alterations in the N-glycan profile of antibodies may serve as biomarkers for the diagnosis of GDM in mothers with a normal BMI, although more evidence is needed. By incorporating glycan-based biomarkers into clinical practice, healthcare providers can improve early detection, personalize management strategies, and potentially mitigate adverse pregnancy outcomes associated with obesity and GDM.
Groove Pancreatitis in Focus: Tumor-Mimicking Phenotype, Diagnosis, and Management Insights
Authors: Balaban Marina , Balaban Daniel Vasile , Mănucu George , Bucurică Săndica Nicoleta , Costache Raluca Simona , Ioniță-Radu Florentina , Jinga Mariana , Gheorghe Cristian
Published: February 2025

Background/Objectives: Groove pancreatitis (GP) is an uncommon pancreatic condition implying a challenging differential diagnosis. This study aims to comprehensively evaluate the main risk factors, clinical presentation, imaging and endoscopic characteristics of patients with GP, providing insights into an effective diagnostic approach and therapeutic strategies. Methods: A retrospective analysis was conducted on patients diagnosed with GP, with demographic and clinical data collected. The diagnostic route was followed by an upper endoscopy and was finally confirmed by cross-sectional imaging. In patients with high malignancy suspicion or with an uncertain diagnosis, a pancreatic endoscopic ultrasound (EUS) was further performed. According to imaging features, we divided patients into two categories: with and without tumor-like appearance. Results: Altogether, 23 patients were included, 11 in the tumor-like category, and 12 in the non-tumor-like group; 95.6% were men, 78.2% alcohol consumers, and 73.9% smokers. In both groups, the main symptom was abdominal pain, followed by nausea and vomiting. The most frequent finding at upper endoscopy was edematous duodenal mucosa (16 patients, 80%), followed by mucosal hyperemia (8 patients, 40%). The main finding at cross-sectional imaging was duodenal wall thickening (14 patients, 60.9%), followed by pancreatic head enlargement and duodenal wall cysts (both seen in 12 patients, 52.2%). The EUS predominantly showed duodenal wall thickening (13 patients, 68.4%), and intramural and paraduodenal cysts (10 patients, 52.6%). Conclusions: GP predominantly affects men with a history of chronic alcohol and tobacco use. Its primary diagnostic challenge lies in distinguishing it from pancreatic carcinoma, with an accurate diagnostic workup being crucial in clinical practice.
Alexithymia Increases the Headache Pain Index in Women with Migraine: Preliminary Results
Authors: Rota Eugenia , Cavagnetto Elisa , Immovilli Paolo , Morelli Nicola , Salari Pavel , Battaggia Alessandro
Published: February 2025

Background: Alexithymia is characterized by a deficit in identifying and communicating feelings. Emerging evidence suggests that it is highly prevalent in migraine, where it could affect the pain expression. This pilot study on female migraineurs aimed at assessing any relationship between alexithymia and headache attacks in terms of frequency and pain intensity. Methods: All the patients (42) who fulfilled the diagnostic criteria for migraine were enrolled in this pilot, observational, cross-sectional study after having obtained written informed consent. A psychological assessment was made of each patient to identify any alexithymia using the TAS-20 scale, for anxiety/mood comorbidity (the STAI-Y1, STAI-Y2, and BDI-II) and for migraine-related disability (the HIT-6). An HPI index (attack frequency x pain intensity) was also calculated for each patient, based on their headache diaries. A multivariate analysis was performed to investigate any association among the TAS-20 score, HPI score, and the following covariates: BDI-II, STAI-Y1, STAI-Y2, HIT-6 scores, age, education, and disease duration. Results: Overall, 35.6% of the sample were given a diagnosis of alexithymia. After removing a subgroup of 7 subjects with HPI > 100, with more severe psychiatric comorbidity and a longer disease duration from the whole sample, a multivariate analysis detected a statistically significant (p = 0.010) association between the HPI and TAS-20 scores. Conclusions: This pilot study suggests that alexithymia may play a role in increasing the frequency and pain intensity of migraine attacks, consequently worsening disability in female migraineurs. Further studies are required to confirm this finding.
Arrhythmia-Induced Cardiomyopathy: Predictors of Improvement in Left Ventricular Systolic Function After Catheter Ablation
Authors: Varrenti Marisa , Bonvicini Eleonora , Baroni Matteo , Gigli Lorenzo , Carbonaro Marco , Garofani Ilaria , Colombo Giulia , Vargiu Sara , De Filippo Valentina , Giordano Federica , Falco Raffaele , Frontera Antonio , Menè Roberto , Preda Alberto , Mazzone Patrizio , Guarracini Fabrizio
Published: February 2025

Background: Arrhythmia-induced cardiomyopathy (AIC) is a relatively common yet poorly understood cardiac condition that occurs when arrhythmias cause left ventricular systolic dysfunction, which can be reversed with the resolution of the arrhythmia. Catheter ablation serves as a cornerstone treatment for eliminating the arrhythmic trigger. However, the variability in left ventricular recovery following ablation highlights the need to identify reliable predictors of reverse remodeling. Methods: This review explores key studies on AIC patients undergoing catheter ablation, primarily derived from observational cohorts, to identify significant predictors of left ventricular function recovery. Results: While echocardiography and cardiac magnetic resonance imaging remain the primary diagnostic modalities, additional insights have emerged from electrocardiograms and laboratory biomarkers. Despite these advancements, a comprehensive framework for identifying optimal candidates for ablation remains lacking. Conclusions: By synthesizing existing evidence, this review aims to pinpoint the most robust predictors of systolic recovery in AIC patients following ablation.
Evaluation of the Effectiveness of a Mouthwash Containing Spilanthol and Cannabidiol on Improving Oral Health in Patients with Gingivitis—Clinical Trial
Authors: Kiełbratowski Maksymilian , Kuśka-Kiełbratowska Anna , Mertas Anna , Bobela Elżbieta , Wiench Rafał , Kępa Małgorzata , Trzcionka Agata , Korkosz Rafał , Tanasiewicz Marta
Published: February 2025

Background/Objectives: Plaque-associated gingivitis is widely regarded as a local inflammatory condition initiated by the accumulation of a non-specific dental biofilm in the interaction with the host immune system. The initial symptom noticed by the patient is bleeding gums. The use of mouthwash can serve to supplement mechanotherapy. However, there is an increasing interest in mouthwashes comprising natural ingredients, including cannabidiol (CBD) and spilanthol. The objective of this study was to evaluate the effect of an oral rinse containing spilanthol and CBD oil compared to a rinse containing tea tree oil on the oral microbiota and the values of selected oral status indicators in patients with gingivitis. Methods: The study included 40 patients treated with a rinse containing tea tree oil (TTO)/TTO + spilanthol + CBD for a period of 42 days. Patients rinsed their mouth twice daily for 30 s. The patients’ oral microbiome was assessed before and after treatment, and bleeding on probing (BOP) and approximal plaque index (API) were assessed. The study was double-blind. Results: API and BOP were reduced in all groups, both the test and control. The most significant decrease in baseline BOP-1 scores was observed in test groups A and D (p = 0.005062 and p = 0.005062, respectively). A significant difference in API improvement was observed between the initial and final visits in the test (A, D) and control (B, C) groups (p = 0.012516, p = 0.005062, p = 0.004028, p = 0.003172, respectively). Conclusions: Firstly, the use of a mouthwash containing cannabidiol (CBD) and spilanthol was demonstrated to be efficacious in the maintenance of oral microbiota homeostasis. Secondly, the combination of TTO with spilanthol and CBD in the rinse was shown to result in a more significant reduction in selected oral health parameters (BOP and API) and anti-inflammatory effects when compared to a rinse with TTO alone. It should be noted that this is a pilot study and will continue.
Access Options for Transcatheter Aortic Valve Replacement
Authors: Chidester Jeffrey , Donisan Teodora , Desai Parth V. , Banthiya Sukriti , Zaghloul Ahmed , Jessen Michael E. , Park Ki , Tan Weiyi , Tsai Shirling , Huffman Lynn , Bavry Anthony A. , Kumbhani Dharam J. , Goyal Amit
Published: February 2025

Transcatheter aortic valve replacement (TAVR) was introduced in 2002 and has become integral in the management of aortic stenosis. As an alternative to surgical aortic valve replacement, it relies heavily on safe access to the aortic annulus for implantation of a valve prosthesis. Throughout its development and in current practice, the transfemoral (TF) arterial route for retrograde valve delivery has been the primary approach. However, this route is not appropriate for all patients, which has led to the development of multiple alternate access options. This review discusses the development of access for TAVR, followed by a thorough discussion of TF access. The commercially available products, preprocedural planning, closure techniques, and procedural complications are all discussed. We also describe the various alternate access routes with particular emphasis on the most recently developed route, transcaval access (TCv), with focus on procedural indications, technical considerations, and comparative outcomes. As TAVR technology, indications, and availability all expand, the knowledge and implementation of safe access are of utmost importance.