bluuebunny/crossref_metadata_2025 huggingface dataset:
Association Analysis of METTL23 Gene Polymorphisms with Reproductive Traits in Kele Pigs
Authors: Sun Jie , Wang Chunyuan , Wu Yan , Xiang Jin , Zhang Yiyu
Published: August 2024

Methyltransferase-like 23 (METTL23) is a kind of RNA methyltransferase that catalyzes the methylation transfer to the N6-adenosine of RNA, serving as one of the key mediators in this process. However, the METTL23 gene has been poorly researched in pigs. In this study, we investigated the genetic effects of METTL23 single-nucleotide polymorphism(SNPs) on reproductive traits in Kele pigs. The DNA was extracted from 228 healthy multiparous Kele sows, and Sanger sequencing revealed three SNPs, g.4804958 G > T (intron 2), g.4805082 C > T (exon 2), and g.4806821 A > G (exon 3). The polymorphism information content (PIC) for each SNP was 0.264, 0.25, and 0.354, indicating moderate polymorphism (0.25 < PIC < 0.5) and providing genetic information. Linkage disequilibrium analysis showed no strong linkage disequilibrium between the three SNPs. The association analysis revealed that in the SNP g.4804958 G > T individuals with the GG genotype had a significantly higher number of piglets born alive, litter birth weight, number of weaned piglets, and weaning litter weight compared to those with the TT genotype (p < 0.05). Individuals with the GG genotype in the SNP g.4806821 A > G group had significantly higher litter birth weight and average birth weight than those with the AA genotype (p < 0.05). The H4H4 diplotype showed significant effects on the number of piglets born alive, litter birth weight, number of weaned piglets, weaning litter weight, and weaning weight (p < 0.05). Together, the METTL23 gene could be used as a candidate gene for the selection of reproductive traits in Kele pigs.
Genome-Wide Identification and Expression Analysis of Growth-Regulating Factor Family in Sweet Potato and Its Two Relatives
Authors: Huang Wenhui , Lin Xiongjian , Li Zhenqin , Mai Jinglin , Hu Mengqin , Zhu Hongbo
Published: August 2024

Growth-regulating factor (GRF) is a multi-gene family that plays an important role in plant growth and development and is widely present in plants. Currently, GRF gene members have been reported in many plants, but the GRF gene family has not been found in sweet potato. In this study, ten GRF genes were identified in sweet potato (Ipomoea batatas), twelve and twelve were identified in its two diploid relatives (Ipomoea trifida) and (Ipomoea triloba), which were unevenly distributed on nine different chromosomes. Subcellular localization analysis showed that GRF genes of sweet potato, I. trifida, and I. triloba were all located in the nucleus. The expression analysis showed that the expression of IbGRFs was diverse in different sweet potato parts, and most of the genes were upregulated and even had the highest expression in the vigorous growth buds. These findings provide molecular characterization of sweet potato and its two diploid relatives, the GRF families, further supporting functional characterization.
Mineralogical and Engineering Properties of Soils Derived from In Situ Weathering of Tuff in Central Java, Indonesia
Authors: Indrawan I Gde Budi , Tamado Daniel , Abrar Mifthahul , Warmada I Wayan
Published: August 2024

This paper presents the results of borehole investigations and laboratory tests carried out to characterize the soils derived from in situ weathering of tuff in Central Java, Indonesia. The 70 m thick weathering profile of the Quaternary tuff consisted of residual soil and completely to highly decomposed rocks. The relatively low dry unit weight and cohesion but high water content, porosity, plastic and liquid limits, and angle of internal friction of the soils in the present study were related to the dominance of halloysite clay minerals. The established relationships to predict soil shear strength parameters from the soil plasticity index and standard penetration test (SPT) N-values were examined, and linear and non-linear relationships for soils derived from in situ weathering of tuff were proposed.
Comfortable Seatbelts for Pregnant Women with Twins in Japan: A Cross-Sectional Observational Study on Seatbelt Usage
Authors: Tsuchikawa Sachi , Miyajima Yui , Tateoka Yumiko , Hitosugi Masahito
Published: August 2024

Optimal seatbelt practices for pregnant women with twins at different gestational ages remain uncertain. To offer recommendations for a comfortable seatbelt system, this cross-sectional observational study explored seatbelt usage and driving habits among women with twins across various pregnancy stages through an online survey that explored driving conditions and comfortable seatbelts at different stages of pregnancy. Women who drove daily before their pregnancy with twins decreased their driving frequency as the pregnancy progressed. Correct seatbelt usage was lower and no seatbelt usage was higher among pregnant women with twins than those with singleton pregnancies. They adapted their seatbelt-wearing techniques to minimise pressure on the chest in the first and third trimesters and the abdomen from the second trimester onwards. The comfortable seatbelts were those that could alleviate belt pressure, featuring waist belts to reduce pressure, wider belts to avoid localised pressure, and shoulder belts resembling a backpack type. When wearing a seatbelt, avoiding pressure on the thorax and abdomen is key for pregnant women with twins. This study suggests that the suitability of driving for pregnant women with twins in their last trimester and the reliability of seatbelts designed for such women should be further examined and validated.
Knowledge, Attitude, and Perception towards Autism Spectrum Disorders among Parents in Sakaka, Al-Jouf Region, Saudi Arabia: A Cross-Sectional Study
Authors: ALRuwaili Bashayer Farhan , Alrashdi Bader Abdullah T. , Mallick Ayesha , Alruwaili Thamer Alshami Marghel , Alanazi Muhannad Faleh , Alruwaili Hanan Farhan S. , Alanazi Wael Faleh , Alanazi Waad Mudhhi , Altaymani Abdullah Fehaid Mukhlef
Published: August 2024

Parents are an essential element of family intervention for all children, including those with autism spectrum disorders (ASDs). We can better understand and address parents’ knowledge gaps about ASD through in-depth research and inquiry into parents’ current level of understanding, attitude, and perception. We aimed to assess the knowledge, attitude, and perception of ASD and influencing factors towards ASD among a group of parents with and without a child diagnosed with ASD in Sakaka, Al-Jouf Region, Saudi Arabia. Using the cross-sectional study design, information from the parents was gathered using a pretested questionnaire that included validated scales for measuring knowledge, attitudes, and perceptions related to ASD. The required number of participants was selected using the convenience sampling method. We used Spearman’s correlation test to determine the strength and direction of correlation between each domain. As a last step, we analyzed the influencing factors using binomial logistic regression. Among the 400 participants, 41.2% had high knowledge, 69.1% had a positive attitude, and 60.3% had a high perception of ASD. We found that knowledge was significantly higher among the parents with autistic individuals in the family (p = 038). The high and positive attitude was significantly greater among females (p = 0.010) and parents with high income (p = 0.007), and the perception was significantly associated with females (p = 0.037) and highly educated participants (p = 0.046). Furthermore, we found a positive correlation between knowledge, attitude, and perception. Overall, only less than half of the participants had a high knowledge of ASD. Hence, we recommend awareness-raising programs for the parents in this region. Furthermore, a prospective study involving parents from all provinces of Saudi Arabia is recommended.
Effects of Concurrent Training on Resuscitation and Cognitive Performance in Paramedics—A Pilot Study
Authors: Brandt Tom , Schittenhelm Andrea , Kuhn Botelho Daniel , Müller Tim , Schmidt Annette
Published: August 2024

Paramedics work under physically and cognitively demanding conditions to provide emergency care. As physical fitness could positively impact the quality of patient care, we investigated within a pilot study whether concurrent training (CT) affects work-related performance parameters in paramedics. At baseline (T1), 16 paramedics performed cardiopulmonary resuscitation whereby resuscitation performance (RP; total resuscitation quality, compressions with correct frequency, and correct ventilation), cognitive performance (CP; reaction time, divided attention, and working memory), and heart rate variability (HRV) were assessed (pre-exertion). Then, participants climbed seven floors carrying 20 kg of gear before completing the same assessments again (post-exertion). The baseline testing was followed by a CT intervention (12 weeks, three sessions/week). After the intervention (T2), the two-stage testing was repeated. We analyzed whether the pre-exertion and post-exertion values, as well as the difference between the pre-exertion and post-exertion values, changed from T1 to T2. Nine paramedics (male: N = 7; age = 26.3 (SD = 8.17) years) took part in the study. The comparison of the pre-exertion values showed significantly better reaction times (p = 0.001) and divided attention (p = 0.02) and a trend toward greater working memory and RP parameters at T2. Regarding the post-exertion values, significant improvements in working memory (p = 0.03) and a trend toward improved reaction time, divided attention, and RP occurred at T2. The difference between the pre- and post-exertion values did not change for any parameter from T1 to T2. HRV decreased significantly from pre- to post-exertion (T1: p = 0.01, T2: p = 0.01). These results indicate that CT is a promising training concept to improve RP and CP in paramedics and should therefore be investigated further to increase patient care quality.
A Survey of Utilization and Satisfaction of Korean Subfertility Treatment among Korean Women
Authors: Park Minjung , Shin Seungwon , Kim Jihye , Kim Jong-hyun , Kim Dong-Il , Sung Soo-Hyun , Park Jang-Kyung
Published: August 2024

Low fertility is a critical social problem worldwide, and infertility has a prevalence of 15%. This cross-sectional study aimed to understand the factors affecting the usage and satisfaction of Korean medicine (KM) in subfertile women. An online survey was conducted from 3 November to 8 November 2021. The survey collected basic information, KM treatment experience, and satisfaction from women who experienced poor pregnancy. The t-test and chi-square test (χ2-test) were used to determine the overall characteristics of the subjects and factors affecting the utilization and satisfaction of KM treatment. Of the total of 29,465 people, 4922 read the survey email, and 601 responded. After excluding 51 respondents with questionable response patterns, 550 respondents were included in the final analysis. Of these, 43.1% (n = 237) had experience with conventional treatment, and 16.5% (n = 91) had received KM treatment. The group that received both KM treatment and CM treatment (n = 59, 24.9%) was significantly more prevalent than the group that received KM treatment alone (n = 32, 10.2%) (p = 0.00). Women who had given birth more than once or held a master’s degree were significantly more willing to participate in the ‘KM Support Project for Subfertility’ program. Our findings suggest that subfertile patients prefer integrated treatment that combines KM and CM treatments. Further studies are needed to assess the status of integrative medicine treatment, satisfaction with each KM intervention, factors for low satisfaction, and patient requirements.
Influence of Health Insurance Coverage on the Survival Rate for Primary Total Knee Arthroplasty: Minimum 5-Year Follow-Up Analysis
Authors: Seo Jae-Sung , Bae Jung-Kwon , Shin Seong-Kee , Ryu Hyung-Gon , Kim Kyu Jin , Cho Seung Yeon
Published: August 2024

This study investigated whether differences in survival rates and clinical outcomes exist in patients undergoing TKA by insurance type: National Health Insurance (NHI) vs. Medical Aid Program (MAP). This study conducted a retrospective analysis of 762 TKAs (NHI, n = 505; MAP, n = 257) with a mean follow-up of 8.4 ± 1.8 years. Patient-reported outcomes (PROMs) were evaluated using the American Knee Society’s (AKS) score at the final follow-up. The survival rate of each group was analyzed using Kaplan–Meier survival analysis. Any postoperative complications and readmissions within 90 days of discharge were recorded and compared between the groups. There were no between-group differences in pre- to postoperative improvement in AKS scores. The estimated 10-year survival rates were 98.5% in the NHI group and 96.9% in the MAP group, respectively, with no significant differences (p = 0.48). However, the length of hospital stay (LOS) was significantly longer in the MAP group than in the NHI group (13.4 days vs. 13.1 days, p = 0.03), and the transfer rate to other departments was significantly higher in the MAP group than in the NHI group (3.9% vs. 1.4%, p = 0.04). Readmission rates for orthopedic complications for 90 days were 3.0% in the NHI group and 3.5% in the MAP group, respectively (p = 0.67). Patients’ insurance type showed similar survival rates and clinical outcomes to those of primary TKA at a mean follow-up of 8.4 years, but the LOS and rate of transfer to other departments during hospitalization were influenced by insurance type.
Stable Isotopic Evidence of Paleorecharge in the Northern Gulf Coastal Plain (USA)
Authors: Haile Estifanos , Currens Benjamin J. , Fryar Alan E.
Published: August 2024

Stable isotope abundances (δ18O and δ2H) in regional aquifers can provide important paleoclimate information. However, identifying paleoclimate signals can be complicated by cross-formational mixing and, potentially, by isotopic diffusion between aquifers and confining units. We examine controls on δ18O and δ2H distributions in the Wilcox aquifer of the northern Gulf Coastal Plain (USA). We sampled groundwater for δ18O, δ2H, Cl−, and 36Cl along a ~300 km downgradient transect. We developed a simplified, 1D numerical model of groundwater flow and 18O transport to assess the possible importance of isotopic diffusion between the aquifer and its confining units. Along the inferred flowpath, δ18O and δ2H values were depleted by as much as 1.3 and 8.2‰, respectively, as the Wilcox aquifer transitioned from unconfined to confined. However, they then gradually rose farther downgradient by up to 1.1 and 8.6‰. Chlorine-36 analyses and 14C analyses (from other studies) indicate that groundwater ages range from ~103 yr to ~8 × 105 yr. Modeling results indicate that the effect of diffusion on isotopic abundances is limited, whereas Cl− data indicate that cross-formational flow is limited. Therefore, we posit that confined groundwater in our study reflects a Pleistocene paleorecharge signal.
2D and 3D Modeling of Resistivity and Chargeability to Identify the Type of Saturated Groundwater for Complex Sedimentary Facies
Authors: Masria Ali , Alshammari Talal Obaid , Ghareeb Mohamed , Seif Ahmed Khaled , Abd-Elmaboud Mahmoud E. , Ammar A. I.
Published: August 2024

Determining the type and properties of saturated groundwater for complex sedimentary facies, as well as the various properties of these sedimentary facies, requires extensive geological, hydrogeological, and geophysical studies. Therefore, identifying the different types of subsurface deposits and their physical properties as well as their geological, hydrogeological, and structural settings are the interesting features in this study which play an important role in achieving its objectives. To achieve these objectives, the Direct Current (DC) resistivity method and the Direct Current Time-Domain Induced Polarization (DC-TDIP) method were used. These two methods were applied because they are complementary methods, one of which is more accurate in sediments saturated with fresh water (the DC resistivity method) and the other in sediments saturated with salt water (DC-TDIP method). Also, the DC-TDIP method was applied to avoid ambiguity in the resistivity results, as well as their results were compared with the available geological and hydrogeological field data. Accordingly, 2D and 3D resistivity values were designed to describe the hydro-lithological environment of the recorded sediments, and their hydrogeoelectric properties and groundwater zones were also identified and divided. Also, 2D and 3D chargeability values were designed to distinguish between sediments, their depositional facies, and their saturated water properties. These values also succeeded in separating clay from non-clay layers, and clay layers from layers containing salt water. Therefore, it was found that the integration between the two methods helped in identifying and visualizing the characteristics of the sediments and determining their facies and their water content, which helped in understanding the complex sedimentary facies recorded in the study area as well as identifying the types and characteristics of groundwater contained in these facies. Therefore, it can be recommended to apply the previous methodology and include the two geophysical methods and their results to study complex facies deposits and determine their water content and type, especially in similar depositional environments that are located next to a source of salt water mixed with other types of water.
Strategies for Coping with Minority Stress among Queer Young Adults: Usage Frequency, Associations with Demographics, and Mental Health
Authors: Xu Yinuo , Hall William J. , Scott McRae , Gao Yutong , Chiang Pin-Chen , Williams Denise Yookong , Srivastava Ankur , Ramon Magdelene E. , Englert Adam R.
Published: August 2024

Queer young adults report significantly higher levels of anxiety and depression than their heterosexual counterparts, which is linked to sexual minority stress. Therefore, it is important to understand the coping strategies employed by this population to navigate minority stress and how coping strategies may impact mental health outcomes. Drawing from a U.S. national diverse sample of 387 queer young adults (ages 18–39 years), we analyzed descriptive results of 11 behavioral strategies to cope with minority stress and used ordered logistic and linear regression to examine the following objectives: the frequency of the use of each coping strategy, and the associations between each strategy and demographic characteristics as well as depression and anxiety. Results revealed that avoidance and talking with friends were the most frequently utilized coping strategies, while prayer/religious activities and counseling/psychotherapy/support groups were infrequently used. We examined utilization preferences of coping strategies across demographic factors (e.g., assigned sex at birth and sexual orientation). The use of counseling/psychotherapy/support group was positively associated with mental health symptoms, while exercise and mindfulness/mediation were associated with lower mental health symptoms. Our findings provide insights for mental health researchers and professionals in selecting appropriate coping strategies for queer young adults in prevention and intervention efforts.
Suicide Risk Factors in High School Students
Authors: Gómez Delgado Guillermo , Ponce Rojo Antonio , Ramírez Mireles Jaime Eduardo , Carmona-Moreno Felipe de Jesús , Flores Salcedo Claudia Cecilia , Hernández Romero Aurea Mercedes
Published: August 2024

In Mexico, suicide has become an important public health problem, representing the third leading cause of death in the adolescent population. Suicidal behavior in adolescents is associated with the interaction of complex relationships between personal, interpersonal, and sociocultural factors. Through a quantitative, descriptive, and correlational cross-sectional study, the present study aimed to analyze the prevalence and risk factors associated with suicidal ideation among adolescents from different high schools of the high school system (SEMS) of the University of Guadalajara, in response to the psychosocial impact of the COVID-19 pandemic. A descriptive statistical analysis was carried out on the data obtained from the 3583 students surveyed, followed by a principal component analysis (PCA) to identify closely related social, emotional, and behavioral variables. The PCA yielded eight principal components, which together represent 75.42% of the variance across psychometric tests. A multiple linear regression analysis was used, with a regression value (R2) of 0.4811, indicating that the explanatory model can predict 48.1% of the variability in suicidal ideation, with a statistical significance level of 0.05. According to the studies conducted, 19% (688 students) showed indicators of high suicide risk and 26.8% (960 students) showed moderate risk. Depression, mental health, health-related quality of life, physical and psychological well-being, and mood and emotions are the most influential factors in suicidal ideation.
Efficient and Verifiable Range Query Scheme for Encrypted Geographical Information in Untrusted Cloud Environments
Authors: Mei Zhuolin , Zeng Jing , Zhang Caicai , Yao Shimao , Zhang Shunli , Wang Haibin , Li Hongbo , Shi Jiaoli
Published: August 2024

With the rapid development of geo-positioning technologies, location-based services have become increasingly widespread. In the field of location-based services, range queries on geographical data have emerged as an important research topic, attracting significant attention from academia and industry. In many applications, data owners choose to outsource their geographical data and range query tasks to cloud servers to alleviate the burden of local data storage and computation. However, this outsourcing presents many security challenges. These challenges include adversaries analyzing outsourced geographical data and query requests to obtain privacy information, untrusted cloud servers selectively querying a portion of the outsourced data to conserve computational resources, returning incorrect search results to data users, and even illegally modifying the outsourced geographical data, etc. To address these security concerns and provide reliable services to data owners and data users, this paper proposes an efficient and verifiable range query scheme (EVRQ) for encrypted geographical information in untrusted cloud environments. EVRQ is constructed based on a map region tree, 0–1 encoding, hash function, Bloom filter, and cryptographic multiset accumulator. Extensive experimental evaluations demonstrate the efficiency of EVRQ, and a comprehensive analysis confirms the security of EVRQ.
Systematic Literature Review of Blockchain Technology’s Technical Challenges: A Tertiary Study
Authors: Chandan Anulipt , Potdar Vidyasagar , John Michele
Published: August 2024

Research into blockchain technology has expanded significantly due to its potential to enhance transparency and trust in business operations, yet adoption is limited by technical challenges like scalability, consensus mechanisms, and security, as well as non-technical barriers such as governance and standardization. This study synthesizes findings from 21 systematic literature reviews (SLR) published between January 2009 and January 2024, focusing on the technical performance of blockchain and its integration with technologies like cloud computing and machine learning. The analysis highlights advancements in scalability, security, privacy, and smart contracts alongside areas needing more research, such as interoperability and integration with legacy systems. This comprehensive review aims to map current technological developments, identify ongoing limitations, and identify specific technical challenges that impede broader adoption. The findings direct future research towards overcoming these barriers, facilitating the smoother implementation of blockchain across various business sectors.
Fabric Defect Detection in Real World Manufacturing Using Deep Learning
Authors: Nasim Mariam , Mumtaz Rafia , Ahmad Muneer , Ali Arshad
Published: August 2024

Defect detection is very important for guaranteeing the quality and pricing of fabric. A considerable amount of fabric is discarded as waste because of defects, leading to substantial annual losses. While manual inspection has traditionally been the norm for detection, adopting an automatic defect detection scheme based on a deep learning model offers a timely and efficient solution for assessing fabric quality. In real-time manufacturing scenarios, datasets lack high-quality, precisely positioned images. Moreover, both plain and printed fabrics are being manufactured in industries simultaneously; therefore, a single model should be capable of detecting defects in all kinds of fabric. So training a robust deep learning model that detects defects in fabric datasets generated during production with high accuracy and lower computational costs is required. This study uses an indigenous dataset directly sourced from Chenab Textiles, providing authentic and diverse images representative of actual manufacturing conditions. The dataset is used to train a computationally faster but lighter state-of-the-art network, i.e., YOLOv8. For comparison, YOLOv5 and MobileNetV2-SSD FPN-Lite models are also trained on the same dataset. YOLOv8n achieved the highest performance, with a mAP of 84.8%, precision of 0.818, and recall of 0.839 across seven different defect classes.
Investigating Credit Card Payment Fraud with Detection Methods Using Advanced Machine Learning
Authors: Chang Victor , Ali Basit , Golightly Lewis , Ganatra Meghana Ashok , Mohamed Muhidin
Published: August 2024

In the cybersecurity industry, where legitimate transactions far outnumber fraudulent ones, detecting fraud is of paramount significance. In order to evaluate the accuracy of detecting fraudulent transactions in imbalanced real datasets, this study compares the efficacy of two approaches, random under-sampling and oversampling, using the synthetic minority over-sampling technique (SMOTE). Random under-sampling aims for fairness by excluding examples from the majority class, but this compromises precision in favor of recall. To strike a balance and ensure statistical significance, SMOTE was used instead to produce artificial examples of the minority class. Based on the data obtained, it is clear that random under-sampling achieves high recall (92.86%) at the expense of low precision, whereas SMOTE achieves a higher accuracy (86.75%) and a more even F1 score (73.47%) at the expense of a slightly lower recall. As true fraudulent transactions require at least two methods for verification, we investigated different machine learning methods and made suitable balances between accuracy, F1 score, and recall. Our comparison sheds light on the subtleties and ramifications of each approach, allowing professionals in the field of cybersecurity to better choose the approach that best meets the needs of their own firm. This research highlights the need to resolve class imbalances for effective fraud detection in cybersecurity, as well as the need for constant monitoring and the investigation of new approaches to increase applicability.
Ethical Challenges and Solutions of Generative AI: An Interdisciplinary Perspective
Authors: Al-kfairy Mousa , Mustafa Dheya , Kshetri Nir , Insiew Mazen , Alfandi Omar
Published: August 2024

This paper conducts a systematic review and interdisciplinary analysis of the ethical challenges of generative AI technologies (N = 37), highlighting significant concerns such as privacy, data protection, copyright infringement, misinformation, biases, and societal inequalities. The ability of generative AI to produce convincing deepfakes and synthetic media, which threaten the foundations of truth, trust, and democratic values, exacerbates these problems. The paper combines perspectives from various disciplines, including education, media, and healthcare, underscoring the need for AI systems that promote equity and do not perpetuate social inequalities. It advocates for a proactive approach to the ethical development of AI, emphasizing the necessity of establishing policies, guidelines, and frameworks that prioritize human rights, fairness, and transparency. The paper calls for a multidisciplinary dialogue among policymakers, technologists, and researchers to ensure responsible AI development that conforms to societal values and ethical standards. It stresses the urgency of addressing these ethical concerns and advocates for the development of generative AI in a socially beneficial and ethically sound manner, contributing significantly to the discourse on managing AI’s ethical implications in the modern digital era. The study highlights the theoretical and practical implications of these challenges and suggests a number of future research directions.
Direct Observation of Feeding Behavior of Adult Tabanidae (Diptera) on Beef Cattle from Khon Kaen Province in Thailand
Authors: Thinnabut Kanchana , Maleewong Wanchai , Tangkawanit Ubon
Published: August 2024

Tabanidae (horse flies and deer flies) are hematophagous insects that cause direct and indirect damage to animal production. The aims of this study were to determine the preferred site, time of day, and duration of tabanid feeding on beef cattle and identify factors related to infestation by tabanids. The population of tabanids was surveyed on certain body parts of the beef cattle (fore udder, tail, navel, leg, dewlap, body, and under) during the morning hours (9.00–10.30 a.m.), midday (12.00–13.30 a.m.), and afternoon (15.30–17.00 p.m.) every day for 10 days. The findings showed that two genera, Tabanus Linnaeus, 1758, and Chrysops Meigen, 1803, landed on the cows. The leg was statistically significantly the most frequent landing site for tabanids (15.067 ± 7.54) compared with other parts. The average feeding duration for each insect was 2.76 ± 1.77 min. The results showed that a significant number of tabanids were present during midday, as compared with the morning and afternoon. Temperature was found to be positively associated with fly abundance. A regression model was derived in this study (y = 4.23x − 116.09). This information is important for tabanid control and prevention in beef cattle.
Linear Morphometry of Male Genitalia Distinguishes the Ant Genera Monomorium and Syllophopsis (Hymenoptera: Formicidae) in Madagascar
Authors: Rasoarimalala Nomena F. , Ramiadantsoa Tanjona , Rakotonirina Jean Claude , Fisher Brian L.
Published: August 2024

Morphometric analyses of male genitalia are routinely used to distinguish genera and species in beetles, butterflies, and flies, but are rarely used in ants, where most morphometric analyses focus on the external morphology of the worker caste. In this work, we performed linear morphometric analysis of the male genitalia to distinguish Monomorium and Syllophopsis in Madagascar. For 80 specimens, we measured 10 morphometric characters, especially on the paramere, volsella, and penisvalvae. Three datasets were made from linear measurements: mean (raw data), the ratios of characters (ratio data), and the Removal of Allometric Variance (RAV data). The following quantitative methods were applied to these datasets: hierarchical clustering (Ward’s method), unconstrained ordination methods including Principal Component Analysis (PCA), Non-Metric Multidimensional Scaling analyses (NMDS), Linear Discriminant Analysis (LDA), and Conditional Inference Trees (CITs). The results from statistical analysis show that the ratios proved to be the most effective approach for genus-level differentiation. However, the RAV method exhibited overlap between the genera. Meanwhile, the raw data facilitated more nuanced distinctions at the species level compared with the ratios and RAV approaches. The CITs revealed that the ratios of denticle length of the valviceps (SeL) to the paramere height (PaH) effectively distinguished between genera and identified key variables for species-level differentiation. Overall, this study shows that linear morphometric analysis of male genitalia is a useful data source for taxonomic delimitation.
Short- and Long-Range Dispersal by Members of the Simulium damnosum Complex (Diptera: Simuliidae), Vectors of Onchocerciasis: A Review
Authors: Cheke Robert A. , Hawkes Frances M. , Carnaghi Manuela
Published: August 2024

Blackfly members of the Simulium damnosum complex are major vectors of the parasite that causes onchocerciasis in Africa and Yemen, with other vector species involved in a few localized areas of Africa and in the Neotropics. Although the life cycle of these blackflies is linked to fast-flowing rivers, they can travel long distances (up to at least 500 km), calling into question how transmission zones are defined. Knowledge of the short- and long-range dispersal of these vectors could inform where control interventions and monitoring are necessary if targets for onchocerciasis elimination are to be met. Yet, research on blackfly dispersal has been limited and fragmented over the last 70 years. Here, we review the literature on the dispersal of onchocerciasis vectors, and we show the need for further research to establish how far larvae can travel downstream; the extent to which adults invade transmission zones; and whether adults migrate in a series of successive short movements or in single long-distance shifts, or use both methods.
Origins of Susceptibility to Insect Herbivores in High-Yielding Hybrid and Inbred Rice Genotypes
Authors: Horgan Finbarr G. , Almazan Maria Liberty P. , Bernal Carmencita C. , Dilla-Ermita Christine Jade , Ardestani Goli , Mundaca Enrique A. , Crisol-Martínez Eduardo
Published: August 2024

Several studies have reported higher damage from insect herbivores to hybrid compared to inbred (pure line) rice. We used a collection of 20 hybrid and 12 inbred genotypes from diverse origins to test the hypotheses that hybrid rice susceptibility is due to (a) the hybrid plant type and/or (b) rice phylogeny. We challenged the genotypes with Nilaparvata lugans (BPH), Sogatella furcifera (WBPH) and Scirpophaga incertulas (YSB) in greenhouse and screenhouse bioassays and monitored herbivores in field plots. We used single nucleotide polymorphic (SNP) markers to assess genetic similarities between the genotypes and found that the hybrids and inbreds formed two distinct clusters regardless of origin. In the screenhouse, hybrids were more susceptible than inbreds to YSB; however, resistant hybrids and susceptible inbreds were also apparent from both the screenhouse and field plots. Plant biomass was the best predictor of susceptibility to YSB. Plant origin had a greater effect than plant type on susceptibility to BPH and WBPH. WBPH was the most abundant planthopper in the field plots where numbers were highly correlated with planthopper fitness in the greenhouse bioassays. Our results provide evidence that high-yielding hybrids that are relatively resistant to herbivores can be achieved through careful breeding. The avoidance of susceptible genotypes during breeding should remain a key element of integrated rice pest management.
Invasive Mechanical Ventilation Is Associated with Worse Right Ventricular Strain in Acute Respiratory Failure Patients
Authors: Wang Shuyuan , Bashir Zubair , Chen Edward W. , Kadiyala Vishnu , Sherrod Charles F. , Has Phinnara , Song Christopher , Ventetuolo Corey E. , Simmons James , Haines Philip
Published: August 2024

Right ventricular (RV) dysfunction is associated with poor prognosis in acute respiratory failure (ARF). Our study evaluates the efficacy of RV strain in detecting RV dysfunction in ARF patients requiring invasive mechanical ventilation (IMV) compared to tricuspid annular plane systolic excursion (TAPSE). In this retrospective study involving 376 patients diagnosed with ARF and requiring IMV, we extracted clinical and outcome data from patient records. RV global longitudinal strain (RVGLS), free wall longitudinal strain (FWLS), and TAPSE were measured retrospectively using speckle tracking echocardiography (STE) and traditional echocardiography, respectively. We divided the cohort into three groups: TTE during IMV (TTE-IMV, 223 patients), before IMV (TTE-bIMV, 68 patients), and after IMV (TTE-aIMV, 85 patients). Multivariable regression analysis, adjusted for covariates, revealed significantly higher RVGLS and FWLS in the groups not on IMV at the time of TTE compared to the TTE-IMV group. Specifically, the TTE-bIMV group showed higher RVGLS (β = 7.28, 95% CI 5.07, 9.48) and FWLS (β = 5.83, 95% CI 3.36, 8.31), while the TTE-aIMV group exhibited higher RVGLS (β = 9.39, 95% CI 6.10, 12.69) and FWLS (β = 7.54, 95% CI 4.83, 10.24). TAPSE did not reveal any significant differences across the groups. Our study suggests an association between IMV and lower RVGLS and FWLS in ARF patients, indicating that IMV itself may contribute to RV dysfunction. RVGLS and FWLS appear to be more sensitive than TAPSE in detecting changes in RV function that were previously subclinical in patients on IMV. Prospective studies with TTE before, during, and after IMV are necessary to assess the primary driver of RV dysfunction and to prognosticate STE-detected RV dysfunction in this population.
Causal Relationship between Chronic Hepatitis B and Stroke in East Asians: A Mendelian Randomization Study
Authors: Zhang Qi , Shen Cancong , Zhang Lei , Wang Maiqiu
Published: August 2024

Both chronic hepatitis B (CHB) and stroke contribute to a high burden of disease in the majority of low- and middle-income countries. Epidemiological studies yield conflicting results on the association between CHB and stroke, and the causal relationship remains inconclusive. This study aimed to assess the causal effects of CHB on stroke and its subtypes in East Asians by Mendelian randomization (MR) analysis. Variants associated with CHB were obtained from a genome-wide association study (GWAS) of Chinese samples as instrumental variables. The summary statistics for stroke in East Asians were derived from the largest published GWAS to date. Two-sample MR analyses were implemented to evaluate the causal effects of CHB on stroke and its subtypes by using the canonical inverse variance weighting method and other supplementary approaches. We observed an association between genetic predisposition to CHB and a decreased risk of large-artery atherosclerotic stroke (odds ratio = 0.872, 95% confidence interval = 0.786–0.967, p = 0.010). The causal effects of CHB on other stroke outcomes were not statistically significant. Evidence for heterogeneity and horizontal pleiotropy were not found in our analyses. This study provides genetic evidence for a negative association between CHB and stroke in East Asians, which helps improve our understanding of the etiology of stroke.
Outcomes of Colonic and Gastric Tube Transplants after Caustic Esophageal Burn in Children: A 33-Year Review
Authors: de Sousa Amaral Michaël , Vasseur Maurer Sabine , Reinberg Olivier , Divjak Natalie , de Buys Roessingh Anthony
Published: August 2024

Introduction: Accidental caustic burns of the esophagus in children represent a significant global health challenge, often necessitating esophageal reconstruction. The aim of this study is to compare the efficacy and morbidity related to esophagus replacement with colonic and gastric tube transplants in a pediatric population followed for caustic stenosis. Methods: This retrospective study was conducted at a tertiary pediatric surgery unit for children treated from January 1989 to December 2022. We compared colonic and gastric tube esophageal replacement. Short term (within 30 days) and mid-term outcomes and complications were reviewed. Statistical evaluation was considered using a Chi-square test for categorical data analysis. Results: A total of 124 children with caustic esophageal burns were included. Among them, 23 (18.5%) had a gastric tube transplant for esophagus replacement and 101 (81.5%) a colonic transplant. During surgical intervention, we found a significantly higher risk of complications when using a colonic transplant (34%, p < 0.001). There was no significant statistical difference in postoperative short term and mid-term complications between the two techniques. Twenty-six (26%) of the children required a reoperation, with a higher risk in the gastric tube transplant group (p < 0.001). Endoscopic dilatation after surgery was also performed on a higher number of children who had received a gastric tube transplant (p = 0.005). Overall, 97.6% recovered full normal oral feeding. Conclusions: We found that colonic and gastric tube replacement are both good options for pediatric esophageal replacement after a caustic injury and show effectiveness over time. Gastric tube transplants carried a slightly higher risk of reoperations and a higher number of dilatations post-surgery. However, our groups are not really comparable, due to the much higher number of colonic transplants. Both surgical options have to be considered during surgery, and the choice depends on the anatomy of the patient. Our future research will focus on assessing long term quality of life and the potential risk of neoplastic complications.
The Long-Term Impact of COVID-19 on Disability after Post-Acute Rehabilitation: A Pilot Study
Authors: Potcovaru Claudia-Gabriela , Salmen Teodor , Potcovaru Ana Mădălina , Săndulescu Ioana-Miruna , Chiriac Ovidiu , Balasa Ana-Cristinel , Diaconu Laura Sorina , Poenaru Daniela , Pantea Stoian Anca , Cinteza Delia , Berteanu Mihai
Published: August 2024

Background: The long-term effect of the 2019 coronavirus (COVID-19) pandemic is not fully known. Severe cases of COVID-19 have resulted in disability that can be assessed in a biopsychosocial manner with the International Classification of Functioning, Disability and Health with the World Health Organization Disability Assessment Schedule 2.0 (WHODAS 2.0) questionnaire. This study aimed to evaluate the long-term effects on disability of COVID-19 three years after post-acute rehabilitation using WHODAS 2.0. Methods: This single-center cohort study included patients with severe COVID-19 who underwent immediate post-discharge post-acute rehabilitation intervention. Three years later, patients were assessed via telephone using the WHODAS 2.0 questionnaire. Results: Of the 69 patients identified in the hospital database, 27 responded. A total of 16 patients refused to respond due to emotional distress. The mean age was 63.4 ± 8.6 years, 81.5% were independent in the community, 55.3% had been previously admitted to the ICU, and the median rehabilitation hospitalization duration was 18 (11.5,24) days. Comorbidities included type 2 diabetes mellitus (DM) (55.5%), grade 2 high blood pressure (62.9%), pressure ulcers (37%), peripheral neurologic deficits (62.9%), and central neurological deficits (14.8%). ICU admission was significantly correlated with advanced rehabilitation needs (measured by the level of the rehabilitation (p < 0.01) and longer hospitalizations (measured by total days in the hospital (p < 0.001). The overall disability score was 35.09%, significantly influenced by DM and central neurological deficits. Conclusions: Central neurological deficits and DM are associated with higher disability scores. Tailored rehabilitation programs, ongoing medical assessment, integrated care models, and patient education are essential for improving long-term outcomes after COVID-19 disease.
Isokinetic Knee Strengthening Impact on Physical and Functional Performance, Pain Tolerance, and Quality of Life in Overweight/Obese Women with Patellofemoral Pain Syndrome
Authors: Hammami Nadhir , Bouzouraa Eya , Ölmez Cengiz , Hattabi Soukaina , Mhimdi Najla , Khezami Mehrzia Amani , Forte Pedro , Sortwell Andrew , Bouassida Anissa , Jemni Monèm
Published: August 2024

Background/Objectives: Patellofemoral syndrome is a common osteoarticular condition that affects many individuals. Various treatment options are available, with a significant emphasis on targeted muscle-strengthening exercises. The purpose of this study was to investigate the effect of isokinetic muscle strengthening on muscle strength, joint range of motion, quality of life, physical performance, and pain tolerance in overweight/obese women with patellofemoral syndrome. Methods: Twenty-four overweight or obese women with patellofemoral syndrome participated in the study during September and October 2023 in a private medical facility for physical medicine and functional rehabilitation. They were randomly assigned to one of two groups for six weeks of isokinetic muscle strengthening. The first group (ISO.G) followed a rehabilitation program combined with isokinetic muscle strengthening. A second group (PCM.G) followed a rehabilitation program that includes an isokinetic protocol in passive compensation movement. The extensors’ peak torque was measured before and after training. Results: The flexors’ peak torque, stair climbing test, 10 m walk, chair lift, monopodal support, goniometric knee flexion test, heel–buttock distance measurement, pain, and quality of life scores improved significantly in both groups. The ISO.G, on the other hand, benefited from a significant increase in quadriceps muscle strength revealed by the extensors’ peak torque. Conclusions: For the treatment of patellofemoral syndrome, isokinetic muscle strengthening in concentric mode appears to have a significant advantage over the classic rehabilitation program with isokinetic passive compensation, particularly in muscle strength gain, in addition to the improvement of joint range of motion, quality of life, physical performance, and pain tolerance. Isokinetic training may be recommended as a beneficial approach for the rehabilitative treatment of patellofemoral pain syndrome in overweight/obese women.
Malnutrition and Allergies: Tipping the Immune Balance towards Health
Authors: Vassilopoulou Emilia , Venter Carina , Roth-Walter Franziska
Published: August 2024

Malnutrition, which includes macro- and micronutrient deficiencies, is common in individuals with allergic dermatitis, food allergies, rhinitis, and asthma. Prolonged deficiencies of proteins, minerals, and vitamins promote Th2 inflammation, setting the stage for allergic sensitization. Consequently, malnutrition, which includes micronutrient deficiencies, fosters the development of allergies, while an adequate supply of micronutrients promotes immune cells with regulatory and tolerogenic phenotypes. As protein and micronutrient deficiencies mimic an infection, the body’s innate response limits access to these nutrients by reducing their dietary absorption. This review highlights our current understanding of the physiological functions of allergenic proteins, iron, and vitamin A, particularly regarding their reduced bioavailability under inflamed conditions, necessitating different dietary approaches to improve their absorption. Additionally, the role of most allergens as nutrient binders and their involvement in nutritional immunity will be briefly summarized. Their ability to bind nutrients and their close association with immune cells can trigger exaggerated immune responses and allergies in individuals with deficiencies. However, in nutrient-rich conditions, these allergens can also provide nutrients to immune cells and promote health.