bluuebunny/crossref_metadata_2025 huggingface dataset:
Dalit and the Historiography of Temple Entry Movements in India: Mapping Social Exclusion and Cultural Subjugation
Authors: Malik Suratha Kumar
Published: February 2022

The period between colonialism and the twenty-first century gives horrible glimpses of temple entry and the violence attached with that. Keeping temple entry as an important issue in mind, here, the article reveals the social exclusion and the cultural subjugation of the Dalits since the colonial period to the present day. Dalits in the colonial period and also in present day are denied their social and religious rights in Hindu religion. The right to enter the temple is a fundamental right of a citizen in a religion like Hinduism. Among the various issues that Dalits have voiced since the colonial period, the issue of temple entry along with untouchability is one of the most important. It is not only a matter of excuse that Dalits till the present day (after seven decades of India’s independence) are not allowed to enter inside the temple in some rural areas of the country. The temple entry bill and the legislations have also been adopted by the princely states and the Parliament of India in different times, but still, Dalits are not allowed to enter the Hindu temples even in various parts of India, for instance, in the Kendrapara district of Odisha. As temple entry is an important issue for Dalits as well as for upper caste Hindus in social and religious life, it is pertinent to revisit the historiography of temple entry movements including the contemporary movements which remain important in religious, social and academic spheres. With the aforementioned backdrop, the article first provides a synoptic view on the historiography of Dalit movements in India and on ‘the Gandhi–Ambedkar debate on caste, untouchability and the issue of temple entry’ as a background for the study, and the latter sections thoroughly explores the historicity of temple entry movements and the social exclusion and cultural subjugation inherited with it since the colonial period to the present day. The article also provides a particular section on the temple entry movement in Odisha (2005–2006) which is based on the empirical works of the author and examines the issue in a critical lens with observations and findings.
Open Latissimus Dorsi Transfer for Irreparable Subscapularis Tendon Tear
Authors: Le Hanneur Malo , Housset Victor , Martinel Vincent , Nourissat Geoffroy
Published: February 2022

Background:

In cases of irreparable subscapularis (SSC) tendon tear in young and active patients, tendon transfers stand as the only surgical option. With a posterior-inferior-medial line of action and a synergistic action with the SSC muscle, the latissimus dorsi (LD) muscle appears to be the most adequate option.

Indications:

Indications include symptomatic and irreparable Lafosse type-IV or type-V SSC lesions in young and active patients with preserved glenohumeral joint.

Technique Description:

With the patient in the supine position under general anesthesia, a deltopectoral approach is used and extended distally so that the pectoralis major (PM) and long head of the biceps (LHB) tendons can be fully exposed and mobilized. The LD tendon is then exposed, freed from its humeral insertion to be prepared in a Krackow fashion, and extensively released from surrounding fasciomuscular adhesions to be freely mobilized proximally. The lesser tuberosity is prepared with a rongeur so that the LD tendon can be secured to cancellous bone on the former SSC insertion site with two rows of anchors. The rotator interval is then closed to prevent any extension of the existing tear to the superior aspect of the rotator cuff. Finally, the LHB is fixed to conjoint tendon in physiologic tension, and the surgical wound is closed.

Results:

With failure and revision rates ranging from 0% to 20%, this transfer seems as safe and reliable as other transfers. Unspecific complications were observed by different teams, but no cases of nerve compressions were reported. Conversely, significantly better functional outcomes are reported when using the LD muscle then when using the PM muscle as donor.

Discussion/Conclusion:

With a rather straightforward surgical technique, the open LD transfer seems to provide satisfactory short- to mid-term outcomes in cases of irreparable and symptomatic SSC tendon tear. Long-term results have yet to be determined, particularly regarding the prevention of glenohumeral osteoarthritis.
NK cell frequencies, function and correlates to vaccine outcome in BNT162b2 mRNA anti-SARS-CoV-2 vaccinated healthy and immunocompromised individuals
Authors: Cuapio Angelica , Boulouis Caroline , Filipovic Iva , Wullimann David , Kammann Tobias , Parrot Tiphaine , Chen Puran , Akber Mira , Gao Yu , Hammer Quirin , Strunz Benedikt , Pérez Potti André , Rivera Ballesteros Olga , Lange Joshua , Muvva Jagadeeswara Rao , Bergman Peter , Blennow Ola , Hansson Lotta , Mielke Stephan , Nowak Piotr , Söderdahl Gunnar , Österborg Anders , Smith C. I. Edvard , Bogdanovic Gordana , Muschiol Sandra , Hellgren Fredrika , Loré Karin , Sobkowiak Michal J. , Gabarrini Giorgio , Healy Katie , Sällberg Chen Margaret , Alici Evren , Björkström Niklas K. , Buggert Marcus , Ljungman Per , Sandberg Johan K. , Aleman Soo , Ljunggren Hans-Gustaf
Published: February 2022

Adaptive immune responses have been studied extensively in the course of mRNA vaccination against COVID-19. Considerably fewer studies have assessed the effects on innate immune cells. Here, we characterized NK cells in healthy individuals and immunocompromised patients in the course of an anti-SARS-CoV-2 BNT162b2 mRNA prospective, open-label clinical vaccine trial. See trial registration description in notes. Results revealed preserved NK cell numbers, frequencies, subsets, phenotypes, and function as assessed through consecutive peripheral blood samplings at 0, 10, 21, and 35 days following vaccination. A positive correlation was observed between the frequency of NKG2C+ NK cells at baseline (Day 0) and anti-SARS-CoV-2 Ab titers following BNT162b2 mRNA vaccination at Day 35. The present results provide basic insights in regards to NK cells in the context of mRNA vaccination, and have relevance for future mRNA-based vaccinations against COVID-19, other viral infections, and cancer.

Trial registration: The current study is based on clinical material from the COVAXID open-label, non-randomized prospective clinical trial registered at EudraCT and clinicaltrials.gov (no. 2021–000175-37). Description: https://clinicaltrials.gov/ct2/show/NCT04780659?term=2021-000175-37&draw=2&rank=1.
Impact of pathobiological diversity of Mycobacterium tuberculosis on clinical features and lethal outcome of tuberculosis
Authors: Mokrousov Igor , Pasechnik Oksana , Vyazovaya Anna , Yarusova Irina , Gerasimova Alena , Blokh Aleksey , Zhuravlev Viacheslav
Published: February 2022

Background

Mycobacterium tuberculosis population in Russia is dominated by the notorious Beijing genotype whose major variants are characterized by contrasting resistance and virulence properties. Here we studied how these strain features could impact the progression of pulmonary tuberculosis (TB) concerning clinical manifestation and lethal outcome.

Results

The study sample included 548 M. tuberculosis isolates from 548 patients with newly diagnosed pulmonary TB in Omsk, West Siberia, Russia. Strains were subjected to drug susceptibility testing and genotyping to detect lineages, sublineages, and subtypes (within Beijing genotype). The Beijing genotype was detected in 370 (67.5%) of the studied strains. The strongest association with multidrug resistance (MDR) was found for epidemic cluster Beijing B0/W148 (modern sublineage) and two recently discovered MDR clusters 1071–32 and 14717–15 of the ancient Beijing sublineage. The group of patients infected with hypervirulent and highly lethal (in a mouse model) Beijing 14717–15 showed the highest rate of lethal outcome (58.3%) compared to Beijing B0/W148 (31.4%; P = 0.06), Beijing Central Asian/Russian (29.7%, P = 0.037), and non-Beijing (15.2%, P = 0.001). The 14717–15 cluster mostly included isolates from patients with infiltrative but not with fibrous-cavernous and disseminated TB. In contrast, a group infected with low virulent 1071–32-cluster had the highest rate of fibrous-cavernous TB, possibly reflecting the capacity of these strains for prolonged survival and chronicity of the TB process.

Conclusions

The group of patients infected with hypervirulent and highly lethal in murine model 14717–15 cluster had the highest proportion of the lethal outcome (58.3%) compared to the groups infected with Beijing B0/W148 (31.4%) and non-Beijing (15.2%) isolates. This study carried out in the TB high-burden area highlights that not only drug resistance but also strain virulence should be considered in the implementation of personalized TB treatment.
Clinical and laboratory characteristics of patients hospitalised with COVID-19: clinical outcomes in Abu Dhabi, United Arab Emirates
Authors: Al Harbi Mariam , Al Kaabi Nawal , Al Nuaimi Asma , Abdalla Jehad , Khan Tehmina , Gasmelseed Huda , Khan Asad , Hamdoun Osama , Weber Stefan
Published: February 2022

Background

Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) was first reported in December 2019. The severity of coronavirus disease 2019 (COVID-19) ranges from asymptomatic to severe and potentially fatal. We aimed to describe the clinical and laboratory features and outcomes of hospitalised patients with COVID-19 within the Abu Dhabi Healthcare Services Facilities (SEHA).

Methods

Our retrospective analysis of patient data collected from electronic health records (EHRs) available from the SEHA health information system included all patients admitted from 1 March to 31 May 2020 with a laboratory-confirmed PCR diagnosis of SARS-CoV-2 infection. Data of clinical features, co-morbidities, laboratory markers, length of hospital stay, treatment received and mortality were analysed according to severe versus non-severe disease.

Results

The study included 9390 patients. Patients were divided into severe and non-severe groups. Seven hundred twenty-one (7.68%) patients required intensive care, whereas the remaining patients (92.32%) had mild or moderate disease. The mean patient age of our cohort (41.8 years) was lower than the global average. Our population had male predominance, and it included various nationalities. The major co-morbidities were hypertension, diabetes mellitus and chronic kidney disease. Laboratory tests revealed significant differences in lactate dehydrogenase, ferritin, C-reactive protein, interleukin-6 and creatinine levels and the neutrophil count between the severe and non-severe groups. The most common anti-viral therapy was the combination of Hydroxychloroquine and Favipiravir. The overall in-hospital mortality rate was 1.63%, although the rate was 19.56% in the severe group. The mortality rate was higher in adults younger than 30 years than in those older than 60 years (2.3% vs. 0.95%).

Conclusions

Our analysis suggested that Abu Dhabi had lower COVID-19 morbidity and mortalities rates were less than the reported rates then in China, Italy and the US. The affected population was relatively young, and it had an international representation. Globally, Abu Dhabi had one of the highest testing rates in relation to the population volume. We believe the early identification of patients and their younger age resulted in more favourable outcomes.
Primary emotions as predictors for fear of COVID-19 in former inpatients with Major Depressive Disorder and healthy control participants
Authors: Sanwald Simon , Widenhorn-Müller Katharina ,   GenEmo Research Group, Gahr Maximilian , Kammer Thomas , Schönfeldt-Lecuona Carlos , Montag Christian , Kiefer Markus
Published: February 2022

Introduction

There are reports of an increase in depressive symptoms and fear during the COVID-19 pandemic, in particular in patients with depression. This study investigates factors related to fear of COVID-19 in former inpatients suffering from depression and healthy controls by assessing variables typically associated with depression and anxiety disorders, i.e. stressful life events (SLEs), the primary emotions SADNESS, PLAY and SEEKING as well as dysfunctional emotion regulation strategies with respect to suppression and reappraisal.

Methods

Data of n = 44 former inpatients suffering from depression and n = 49 healthy controls were collected. The study had a longitudinal design with two measurement points. Before the pandemic, SLEs, primary emotions, emotion regulation and depression severity were assessed. During the pandemic, COVID-19 associated stressors and life events, emotion regulation, depression severity and fear of COVID-19 were assessed.

Results

Fear of COVID-19 and depression severity during the pandemic were significantly higher in former inpatients than in healthy controls. Depression diagnosis, SLEs and depression severity before the pandemic were significant positive predictors of fear of COVID-19. The primary emotion PLAY was a significant negative predictor of fear of COVID-19. Depression severity did not change significantly in healthy controls.

Conclusion

The results show that risk factors for depression might be risk factors for high fear of COVID-19. In addition, a playful personality could help preventing mental stress in pandemic situations. Thus, positivity based interventions could counteract elevated fear scores during a pandemic.
Exploring housing policies in five Swedish municipalities: alternatives and priorities
Authors: Heller Christina , Ekstam Lisa , Haak Maria , Schmidt Steven M. , Slaug Björn
Published: February 2022

Introduction

Housing shortage due to population growth within metropolitan areas, combined with an ageing population, has put pressure on current housing provision in Sweden. Thus, there is an urgent need to develop sustainable housing policies to accommodate the growing number of seniors in accessible home environments. This study aimed to gain an in-depth understanding of how municipalities currently address housing accessibility issues and to explore what types of policy solutions they consider for the future.

Material and methods

Five Swedish municipalities were selected to represent a diversity of the population, housing provision approaches, and geographical areas. To understand current housing policies, two key actors (e.g. public officials, housing adaptation grant managers, city architects, etc.) from each municipality participated in semi-structured interviews (N = 10). Subsequently, those key actors, two senior citizens, and three researchers participated in a research circle to explore future policy solutions. Data were analyzed using content analysis.

Results

The interviews revealed common approaches to deal with housing accessibility issues such as regular renovations and maintenance, individual adaptations based on specific needs, and seeking collaboration with private housing actors on housing provision matters. Possible measures suggested for the future included increasing the national coordination of housing accessibility policies, amending legislation to only allow the construction of housing according to strengthened accessibility standards, and introducing economic incentives for seniors to move from housing with poor accessibility to more accessible accommodations.

Conclusions

Municipalities struggle with the lack of accessible and affordable housing for their ageing population, despite a large variety of policies from economic incentives to research and development policies. The results suggest that collaboration needs to be improved between all actors involved in housing policies. Preventive measures within the current laws may be needed to strengthen the construction of more accessible and affordable housing for populations ageing in place.
Socioeconomic differences in metabolic syndrome development: examining the mediating role of chronic stress using the Lifelines Cohort Study
Authors: Hoveling Liza A. , Liefbroer Aart C. , Bültmann Ute , Smidt Nynke
Published: February 2022

Background

Metabolic syndrome (MetS) development strongly varies based on individuals’ socioeconomic position (SEP), but to date, no studies have assessed the mediating role of perceived stress from long-term difficulties (chronic stress) in this association. The aim of this study is to examine the mediating role of chronic stress in the associations of the SEP measures education, occupational prestige and income, with MetS development, and whether associations between chronic stress and MetS are moderated by sex.

Methods

We used an adult subsample (n = 53,216) from the Lifelines Cohort Study without MetS at baseline. MetS development was measured 3.9 years after baseline (follow-up), and defined according to National Cholesterol Education Program’s Adult Treatment Panel III (NCEP-ATPIII) criteria. Direct associations between SEP, chronic stress and MetS development were estimated using multivariable logistic and linear regression analyses, and were adjusted for age, sex, the other SEP measures, and time between baseline and follow-up. The mediating percentages of chronic stress explaining the associations between SEP and MetS development were estimated using the Karlson-Holm-Breen method.

Results

Upon follow-up, 7.4% of the participants had developed MetS. Years of education and occupational prestige were inversely associated with MetS development. Chronic stress suppressed the association between education and MetS development (5.6%), as well as the association between occupational prestige and MetS development (6.2%). No effect modification of sex on the chronic stress-MetS pathway was observed.

Conclusions

Chronic stress does not explain educational and occupational differences in developing MetS. In fact, individuals with more years of education or higher occupational prestige perceive more chronic stress than their lower SEP counterparts. Further, no difference between males and females was observed regarding the relationship between chronic stress and MetS development.
Reduced dispensing of prescribed antibiotics during the Covid-19 pandemic has not increased severe complications from common infections
Authors: Norman Christer , Svensson Mikaela , Schmidt Ingrid , Bergfeldt Vendela S. , Obeid Ragda , Ternhag Anders , Struwe Johan L.
Published: February 2022

Background

Sweden has seen an accelerated decline in the number of dispensed antibiotic prescriptions from an already low level during the Covid-19 pandemic. This prompted us to explore whether the decrease in antibiotic prescriptions has reached a critically low level and resulted in an increase in treatment of severe complications from common infections. The aim was to study if the accelerated decrease in antibiotic sales has led to an increase in complications in outpatients with common infections.

Method

A population-based nationwide registry study based on the Swedish Prescribed Drug Register and the National Patient Register.

Results

The total number of dispensed antibiotic prescriptions decreased by 17% during 2020 compared to 2019. The decrease was most pronounced in younger age groups and for antibiotics targeting respiratory tract infections. The number of hospital admissions and visits to open specialist care due to pneumonia or complications related to otitis, tonsillitis, or sinusitis decreased by 4–44%. Prescriptions and numbers of visits or admissions due to urinary tract infections and skin infections remained largely unchanged compared to previous years.

Conclusion

No increase in complications due to common bacterial infections could be detected despite an unprecedented decline in dispensed antibiotic prescriptions in outpatient care in 2020. The decrease in dispensed antibiotic prescriptions from pharmacies was probably primarily related to a general decrease in the incidence of respiratory infections due to the recommendations and restrictions implemented to mitigate the Covid-19 pandemic in Sweden. This in return led to fewer doctors’ visits and consequently to fewer occasions to prescribe antibiotics, be they warranted or not.
Endoscopy-assisted anterior cervical debridement combined with posterior fixation and fusion for the treatment of upper cervical spine tuberculosis: a retrospective feasibility study
Authors: Liu Zheng , Xu Zhenchao , Zhang Yilu , Wang Xiyang , Zhang Zhen , Jiang Dingyu , Jia Runze
Published: February 2022

Background

This retrospective study aimed to determine the feasibility and efficacy of endoscopy-assisted anterior cervical debridement combined with posterior fixation and fusion in patients with upper cervical spine tuberculosis.

Methods

Between June 2008 and January 2016, 17 patients (10 men and 7 women) with upper cervical spine tuberculosis underwent endoscopy-assisted anterior cervical debridement combined with posterior fixation and fusion. Anti-tuberculosis treatment was administered for 2–4 weeks preoperatively and 12–18 months postoperatively. The clinical and radiographic data of the patients were analyzed.

Results

The operation was successfully completed in all patients. Neck pain and stiffness were relieved after the surgery in all patients. The mean operation time was 210.0 ± 21.2 min, and the mean intraoperative blood loss was 364.7 ± 49.6 mL. The mean follow-up duration was 68.1 ± 6.7 months. The erythrocyte sedimentation rate returned to normal by 3 months postoperatively. Visual analog scale scores for neck pain were significantly lower postoperatively than preoperatively. All patients had significant postoperative neurological improvement. Patient-reported outcomes, as measured using the Kirkaldy-Willis criteria, were as follows: excellent, 12 patients; good, 4 patients; fair, 1 patient; and poor, 0 patients. Bone fusion was achieved at 10.9 ± 1.9 months after the surgery; no cases of instrument loosening or fracture occurred.

Conclusion

Endoscopy-assisted anterior cervical debridement combined with posterior fixation and fusion is a feasible and effective surgical method for the treatment of upper cervical spine tuberculosis. It can be used to restore upper cervical spine stability and facilitate spinal healing.
Multimorbidity and co-occurring musculoskeletal pain do not modify the effect of the selfBACK app on low back pain-related disability
Authors: Øverås Cecilie K. , Nilsen Tom I. L. , Nicholl Barbara I. , Rughani Guy , Wood Karen , Søgaard Karen , Mair Frances S. , Hartvigsen Jan
Published: February 2022

Background

selfBACK, an artificial intelligence (AI)-based app delivering evidence-based tailored self-management support to people with low back pain (LBP), has been shown to reduce LBP-related disability when added to usual care. LBP commonly co-occurs with multimorbidity (≥ 2 long-term conditions) or pain at other musculoskeletal sites, so this study explores if these factors modify the effect of the selfBACK app or influence outcome trajectories over time.

Methods

Secondary analysis of a randomized controlled trial with 9-month follow-up. Primary outcome is as follows: LBP-related disability (Roland Morris Disability Questionnaire, RMDQ). Secondary outcomes are as follows: stress/depression/illness perception/self-efficacy/general health/quality of life/physical activity/global perceived effect. We used linear mixed models for continuous outcomes and logistic generalized estimating equation for binary outcomes. Analyses were stratified to assess effect modification, whereas control (n = 229) and intervention (n = 232) groups were pooled in analyses of outcome trajectories.

Results

Baseline multimorbidity and co-occurring musculoskeletal pain sites did not modify the effect of the selfBACK app. The effect was somewhat stronger in people with multimorbidity than among those with LBP only (difference in RMDQ due to interaction, − 0.9[95 % CI − 2.5 to 0.6]). Participants with a greater number of long-term conditions and more co-occurring musculoskeletal pain had higher levels of baseline disability (RMDQ 11.3 for ≥ 2 long-term conditions vs 9.5 for LBP only; 11.3 for ≥ 4 musculoskeletal pain sites vs 10.2 for ≤ 1 additional musculoskeletal pain site); along with higher baseline scores for stress/depression/illness perception and poorer pain self-efficacy/general health ratings. In the pooled sample, LBP-related disability improved slightly less over time for people with ≥ 2 long-term conditions additional to LBP compared to no multimorbidity and for those with ≥4 co-occurring musculoskeletal pain sites compared to ≤ 1 additional musculoskeletal pain site (difference in mean change at 9 months = 1.5 and 2.2, respectively). All groups reported little improvement in secondary outcomes over time.

Conclusions

Multimorbidity or co-occurring musculoskeletal pain does not modify the effect of the selfBACK app on LBP-related disability or other secondary outcomes. Although people with these health problems have worse scores both at baseline and 9 months, the AI-based selfBACK app appears to be helpful for those with multimorbidity or co-occurring musculoskeletal pain.

Trial registration

NCT03798288. Date of registration: 9 January 2019
An auto-antibody identified from phenotypic directed screening platform shows host immunity against EV-A71 infection
Authors: Cheng Yu-Wei , Chuang Yung-Chun , Huang Sheng-Wen , Liu Ching-Chuan , Wang Jen-Ren
Published: February 2022

Background

Enterovirus A71 (EV-A71) is a neurotropic virus which may cause severe neural complications, especially in infants and children. The clinical manifestations include hand-foot-and-mouth disease, herpangina, brainstem encephalitis, pulmonary edema, and other severe neurological diseases. Although there are some vaccines approved, the post-marketing surveillance is still unavailable. In addition, there is no antiviral drugs against EV-A71 available.

Methods

In this study, we identified a novel antibody that could inhibit viral growth through a human single chain variable fragment (scFv) library expressed in mammalian cells and panned by infection with lethal dose of EV-A71.

Results

We identified that the host protein α-enolase (ENO1) is the target of this scFv, and anti-ENO1 antibody was found to be more in mild cases than severe EV-A71 cases. Furthermore, we examined the antiviral activity in a mouse model. We found that the treatment of the identified 07-human IgG1 antibody increased the survival rate after virus challenge, and significantly decreased the viral RNA and the level of neural pathology in brain tissue.

Conclusions

Collectively, through a promising intracellular scFv library expression and screening system, we found a potential scFv/antibody which targets host protein ENO1 and can interfere with the infection of EV-A71. The results indicate that the usage and application of this antibody may offer a potential treatment against EV-A71 infection.
China’s long march to malaria elimination: a case of adaptive management
Authors: Wang Duo-quan , Liang Xiao-hui , Lu Shen-ning , Ding Wei , Huang Jing , Wen Xin , Lv Shan , Xiao Ning , Husain Lewis , Zhou Xiao-Nong
Published: February 2022

Since the 1950s, China has transitioned from a malaria pandemic country with tens of millions of annual cases, through phases of local control and elimination, to sustained national malaria elimination efforts. This marks the first time a country in the World Health Organization (WHO) Western Pacific region has been certified malaria-free in more than 3 decades. This article provides an innovative approach to understanding China’s malaria elimination journey. A number of articles and commentaries have analysed the effectiveness of specific technical approaches implemented in China. Our argument is that we need to look beyond these, and consider the ways in which policy development and implementation capacities have been fostered to support the dynamic change management. The article makes a number of arguments. First is the pragmatic adaptiveness of policies and strategies—and implementation capacities. Second, China has invested in building systems as well as capacities to support the elimination of parasitic diseases, including malaria. Third, the country has both benefited from, and contributed to, global health collaboration on malaria elimination. The ongoing work by the authors is identifying a number of key factors.
Dynamic alteration of poroelastic attributes as determinant membrane nanorheology for endocytosis of organ specific targeted gold nanoparticles
Authors: Kulkarni Tanmay , Mukhopadhyay Debabrata , Bhattacharya Santanu
Published: February 2022

Background

Efficacy of targeted drug delivery using nanoparticles relies on several factors including the uptake mechanisms such as phagocytosis, macropinocytosis, micropinocytosis and receptor mediated endocytosis. These mechanisms have been studied with respect to the alteration in signaling mechanisms, cellular morphology, and linear nanomechanical properties (NMPs). Commonly employed classical contact mechanics models to address cellular NMPs fail to address mesh like structure consisting of bilayer lipids and proteins of cell membrane. To overcome this technical challenge, we employed poroelastic model which accounts for the biphasic nature of cells including their porous behavior exhibiting both solid like (fluid storage) and liquid like (fluid dissipate) behavior.

Results

In this study, we employed atomic force microscopy to monitor the influence of surface engineering of gold nanoparticles (GNPs) to the alteration of nonlinear NMPs such as drained Poisson’s ratio, effective shear stress, diffusion constant and pore dimensions of cell membranes during their uptake. Herein, we used pancreatic cancer (PDAC) cell lines including Panc1, AsPC-1 and endothelial cell (HUVECs) to understand the receptor-dependent and -independent endocytosis of two different GNPs derived using plectin-1 targeting peptide (PTP-GNP) and corresponding scrambled peptide (sPEP-GNP). Compared to untreated cells, in case of receptor dependent endocytosis of PTP-GNPs diffusion coefficient altered ~ 1264-fold and ~ 1530-fold and pore size altered ~ 320-fold and ~ 260-fold in Panc1 and AsPC-1 cells, respectively. Whereas for receptor independent mechanisms, we observed modest alteration in diffusion coefficient and pore size, in these cells compared to untreated cells. Effective shear stress corresponding to 7.38 ± 0.15 kPa and 20.49 ± 0.39 kPa in PTP-GNP treatment in Panc1 and AsPC-1, respectively was significantly more than that for sPEP-GNP. These results demonstrate that with temporal recruitment of plectin-1 during receptor mediated endocytosis affects the poroelastic attributes of the membrane.

Conclusion

This study confirms that nonlinear NMPs of cell membrane are directly associated with the uptake mechanism of nanoparticles and can provide promising insights of the nature of endocytosis mechanism involved for organ specific drug delivery using nanoparticles. Hence, nanomechanical analysis of cell membrane using this noninvasive, label-free and live-cell analytical tool can therefore be instrumental to evaluate therapeutic benefit of nanoformulations.

Graphical Abstract
Elevated mid-trimester 4-h postprandial triglycerides for predicting late-onset preeclampsia: a prospective screening study
Authors: Liu Qing , Zhu Zhihong , Cai Wen , Yang Liu , Li ShuangDi , Zhang Jiarong
Published: February 2022

Background

Abnormal maternal lipid concentrations are associated with increased risk of preeclampsia. However, previous studies mainly focused on fasting lipid concentrations, scarce data have been published on the relationship between postprandial triglyceride (TG) concentrations in the second trimester and the risk of preeclampsia. Our aim is to evaluate the potential of triglyceride (TG) concentrations at the time of oral lipid tolerance test (OLTT) measurement in the second trimester to predict preeclampsia and to elucidate the lipid metabolic changes related to these diseases.

Methods

This is a prospective cohort study of Pregnant women at 12–24 weeks of gestation undergone an OLTT in a university affiliated hospital between May 2019 and January 2020. Data were stratified into binaries according to the OLTT results. The receiver operating characteristic (ROC) curve analysis was conducted to determine the optimal cut-off points of TG, HDL-C, LDL-C, sd-LDL, FFA, and BG for predicting preeclampsia.

Results

438 pregnant women were recruited to undergo an OLTT at 12–24 weeks of gestation. Among these, 24 women developed preeclampsia and 414 women remained normotensive. Women who subsequently developed preeclampsia had higher concentrations of 4-h postprandial TG than those who remained normotensive. In the linear logistic regression analyses of potential confounding factors, mid-trimester 4-h postprandial TG concentrations at the time of OLTT measurement were significantly higher in preeclamptic cases than in controls.

Conclusions

Dyslipidemia in the second trimester of pregnancy, particularly postprandial hypertriglyceridemia, appears to be associated with an increased risk of preeclampsia. Mid-trimester 4-h postprandial TG concentration at the time of OLTT measurement may be a potential predictive marker of preeclampsia.

Trial registration Data of registration: 2018/10/15. Date of initial participant enrollment: 2019/05/01. Clinical trial identification number: chiCTR1800018884. URL of the registration site: http://www.chictr.org.cn/showproj.aspx?proj=25526. Data sharing information: The data including individual participant data, detailed study protocols, statistical analysis plans will be shared upon request to the corresponding author.
Posttranslational modifications of proteins are key features in the identification of CSF biomarkers of multiple sclerosis
Authors: Salazar Ivan L. , Lourenço Ana S. T. , Manadas Bruno , Baldeiras Inês , Ferreira Cláudia , Teixeira Anabela Claro , Mendes Vera M. , Novo Ana Margarida , Machado Rita , Batista Sónia , Macário Maria do Carmo , Grãos Mário , Sousa Lívia , Saraiva Maria João , Pais Alberto A. C. C. , Duarte Carlos B.
Published: February 2022

Background

Multiple sclerosis is an inflammatory and degenerative disease of the central nervous system (CNS) characterized by demyelination and concomitant axonal loss. The lack of a single specific test, and the similarity to other inflammatory diseases of the central nervous system, makes it difficult to have a clear diagnosis of multiple sclerosis. Therefore, laboratory tests that allows a clear and definite diagnosis, as well as to predict the different clinical courses of the disease are of utmost importance. Herein, we compared the cerebrospinal fluid (CSF) proteome of patients with multiple sclerosis (in the relapse–remitting phase of the disease) and other diseases of the CNS (inflammatory and non-inflammatory) aiming at identifying reliable biomarkers of multiple sclerosis.

Methods

CSF samples from the discovery group were resolved by 2D-gel electrophoresis followed by identification of the protein spots by mass spectrometry. The results were analyzed using univariate (Student’s t test) and multivariate (Hierarchical Cluster Analysis, Principal Component Analysis, Linear Discriminant Analysis) statistical and numerical techniques, to identify a set of protein spots that were differentially expressed in CSF samples from patients with multiple sclerosis when compared with other two groups. Validation of the results was performed in samples from a different set of patients using quantitative (e.g., ELISA) and semi-quantitative (e.g., Western Blot) experimental approaches.

Results

Analysis of the 2D-gels showed 13 protein spots that were differentially expressed in the three groups of patients: Alpha-1-antichymotrypsin, Prostaglandin-H2-isomerase, Retinol binding protein 4, Transthyretin (TTR), Apolipoprotein E, Gelsolin, Angiotensinogen, Agrin, Serum albumin, Myosin-15, Apolipoprotein B-100 and EF-hand calcium-binding domain—containing protein. ELISA experiments allowed validating part of the results obtained in the proteomics analysis and showed that some of the alterations in the CSF proteome are also mirrored in serum samples from multiple sclerosis patients. CSF of multiple sclerosis patients was characterized by TTR oligomerization, thus highlighting the importance of analyzing posttranslational modifications of the proteome in the identification of novel biomarkers of the disease.

Conclusions

The model built based on the results obtained upon analysis of the 2D-gels and in the validation phase attained an accuracy of about 80% in distinguishing multiple sclerosis patients and the other two groups.
Layering contrasting photoselective filters improves the simulation of foliar shade
Authors: Petrella Dominic P. , Breuillin-Sessoms Florence , Watkins Eric
Published: February 2022

Background

Neutral density shade cloth is commonly used for simulating foliar shade, in which it reduces light intensity without altering spectral quality. However, foliar shade also alters spectral quality, reducing the ratio of red to far-red (R:FR) light, altering the ratio of blue to green (B:G) light, and reducing ultraviolet light. Unlike shade cloth, photoselective filters can alter spectral quality, but the filters used in previous literature have not simulated foliar shade well. We examined the spectral quality of sunlight under color temperature blue (CTB), plus green (PG), and neutral density (ND) filters from LEE Filters, Rosco e-colour + and Cinegel brands either alone or layered, hypothesizing that the contrasting filter qualities would improve simulations. As a proof-of-concept, we collected spectral data under foliar shade to compare to data collected under photoselective filters.

Results

Under foliar shade reductions in the R:FR ratio ranged from 0.11 to 0.54 (~ 1.18 in full sun), while reductions in the B:G ratio were as low as 0.53 in deep shade, or were as high as 1.11 in moderate shade (~ 0.87 in full sun). Neutral density filters led to near-neutral reductions in photosynthetically active radiation and reduced the R:FR ratio similar to foliar shade. Color temperature blue filters simulated the increased B:G ratio observed under moderate foliar shade, but did not reduce the R:FR ratio low enough. On their own, PG filters did not simulate any type of foliar shade. Different brands of the same filter type also had disparate effects on spectral quality. Layered CTB and ND filters improved the accuracy of moderate foliar shade simulations, and layering CTB, PG, and ND filters led to accurate simulations of deep foliar shade.

Conclusions

Layering photoselective filters with contrasting effects on the spectral quality of sunlight results in more accurate simulations of foliar shade compared to when these filters are used separately. Layered filters can re-create the spectral motifs of moderate and deep foliar shade; they could be used to simulate shade scenarios found in different cropping systems. Photoselective filters offer numerous advantages over neutral density shade cloth and could be a direct replacement for researchers currently using neutral density shade cloth.
Six practical recommendations for improved implementation outcomes reporting
Authors: Lengnick-Hall Rebecca , Gerke Donald R. , Proctor Enola K. , Bunger Alicia C. , Phillips Rebecca J. , Martin Jared K. , Swanson Julia C.
Published: February 2022

Background

Implementation outcomes research spans an exciting mix of fields, disciplines, and geographical space. Although the number of studies that cite the 2011 taxonomy has expanded considerably, the problem of harmony in describing outcomes persists. This paper revisits that problem by focusing on the clarity of reporting outcomes in studies that examine them. Published recommendations for improved reporting and specification have proven to be an important step in enhancing the rigor of implementation research. We articulate reporting problems in the current implementation outcomes literature and describe six practical recommendations that address them.

Recommendations

Our first recommendation is to clearly state each implementation outcome and provide a definition that the study will consistently use. This includes providing an explanation if using the taxonomy in a new way or merging terms. Our second recommendation is to specify how each implementation outcome will be analyzed relative to other constructs. Our third recommendation is to specify “the thing” that each implementation outcome will be measured in relation to. This is especially important if you are concurrently studying interventions and strategies, or if you are studying interventions and strategies that have multiple components. Our fourth recommendation is to report who will provide data and the level at which data will be collected for each implementation outcome, and to report what kind of data will be collected and used to assess each implementation outcome. Our fifth recommendation is to state the number of time points and frequency at which each outcome will be measured. Our sixth recommendation is to state the unit of observation and the level of analysis for each implementation outcome.

Conclusion

This paper advances implementation outcomes research in two ways. First, we illustrate elements of the 2011 research agenda with concrete examples drawn from a wide swath of current literature. Second, we provide six pragmatic recommendations for improved reporting. These recommendations are accompanied by an audit worksheet and a list of exemplar articles that researchers can use when designing, conducting, and assessing implementation outcomes studies.
Deep learning tools for the cancer clinic: an open-source framework with head and neck contour validation
Authors: Asbach John C. , Singh Anurag K. , Matott L. Shawn , Le Anh H.
Published: February 2022

Background

With the rapid growth of deep learning research for medical applications comes the need for clinical personnel to be comfortable and familiar with these techniques. Taking a proven approach, we developed a straightforward open-source framework for producing automatic contours for head and neck planning computed tomography studies using a convolutional neural network (CNN).

Methods

Anonymized studies of 229 patients treated at our clinic for head and neck cancer from 2014 to 2018 were used to train and validate the network. We trained a separate CNN iteration for each of 11 common organs at risk, and then used data from 19 patients previously set aside as test cases for evaluation. We used a commercial atlas-based automatic contouring tool as a comparative benchmark on these test cases to ensure acceptable CNN performance. For the CNN contours and the atlas-based contours, performance was measured using three quantitative metrics and physician reviews using survey and quantifiable correction time for each contour.

Results

The CNN achieved statistically better scores than the atlas-based workflow on the quantitative metrics for 7 of the 11 organs at risk. In the physician review, the CNN contours were more likely to need minor corrections but less likely to need substantial corrections, and the cumulative correction time required was less than for the atlas-based contours for all but two test cases.

Conclusions

With this validation, we packaged the code framework and trained CNN parameters and a no-code, browser-based interface to facilitate reproducibility and expansion of the work. All scripts and files are available in a public GitHub repository and are ready for immediate use under the MIT license. Our work introduces a deep learning tool for automatic contouring that is easy for novice personnel to use.
Distal chevron osteotomy versus different operative procedures for hallux valgus correction: a meta-analysis
Authors: Clemente Pablo , Mariscal Gonzalo , Barrios Carlos
Published: February 2022

Introduction

Distal chevron osteotomy is commonly used for the operative treatment of hallux valgus (HV). However, there are several operative procedures that can be used to treat HV. The aim of this meta-analysis was to compare the efficacy of distal chevron osteotomy with different operative procedures.

Materials and methods

A systematic search was conducted using the MEDLINE and EMBASE databases to identify randomized clinical trials (RCTs). The variables were radiological (hallux metatarsal phalangeal angle [HVA] and intermetatarsal angle [IMA]) and clinical (American Orthopaedic Foot & Ankle Society Score [AOFAS]). Heterogeneity was assessed with chi2 and I2 statistics. A random effects model was used for significant heterogeneity. Publication bias was evaluated with funnel plots.

Results

Ten studies involving 985 patients were evaluated in the meta-analysis. Distal chevron osteotomy was associated with a mean IMA correction 2.18° greater than the scarf procedure (MD − 2.18; 95% CI − 3.67, − 0.69; p = 0.004; I2 = 0%). In addition, the proximal chevron was associated with a mean IMA correction 1.08° greater than the distal chevron (MD − 1.08; 95% CI − 1.86, − 0.29; p = 0.007; I2 = 0%). The AOFAS assessment showed an overall advantage of 3.2 points in favor of the Lingdren group compared with distal chevron osteotomy (MD 3.20; 95% CI 0.37, 6.04; p = 0.03; I2 = 0%).

Conclusions

Our findings indicate that distal chevron osteotomy provides a greater HVA correction than scarf osteotomy, and proximal chevron provides a larger IMA correction than distal chevron osteotomy. Lingdren osteotomy provides a greater AOFAS correction than distal chevron osteotomy.

Level of evidence

Level I, meta-analysis.
A cross-sectional study of gender differences in quality of life domains in patients with neurofibromatosis type 1
Authors: Hamoy-Jimenez G. , Elahmar H. A. , Mendoza M. , Kim R. H. , Bril V. , Barnett C.
Published: February 2022

Background

There is limited data regarding gender differences in quality of life between women and men with Neurofibromatosis type 1. We aimed to study differences in quality of life domains between women and men with Neurofibromatosis type 1 living in Canada.

Methods

This is a cross sectional study of adults with Neurofibromatosis type 1 attending a tertiary NF centre at Toronto General Hospital between January 2016 to December 2017. Demographic and clinical data were collected. We compared scores of generic measures (SF-36, EQ-5D-5L, pain interference) and a disease-specific measure (PedsQL-NF1 module) between women and men. We also assessed the relationship between disease visibility scored by an examiner (Ablon’s visibility index) and self-reported perceived physical appearance, stratified by gender.

Results

One hundred and sixty-two participants were enrolled, 92 females and 70 males. Ablon’s index score 1 was in 43% and score 2 in 44%, while only 13% of patients had a score 3. Women had worse scores on the total PedsQL-NF1 scales, and also in the perceived physical appearance, anxiety and emotional health domains. In women, there was a low but significant correlation between Ablon’s index and perceived physical appearance (r = − 0.27, p = 0.01, ANOVA p < 0.001). In men, there was no difference in self-reported physical appearance by Ablon’s index. There were no differences between men and women in the SF-36 or EQ-5D-5L scores.

Conclusion

Women with NF1 reported worse NF1-related quality of life than men, with worse perceived physical appearance, anxiety, and mental health. Perceived physical appearance does not always correlate to disease visibility; therefore, healthcare providers should inquire about body image, physical appearance concerns, and mental health, especially among women with NF1.