bluuebunny/crossref_metadata_2025 huggingface dataset:
Perceptions of and challenges faced by primary healthcare workers about preconception services in rural India: A qualitative study using focus group discussion
Authors: Chutke Amruta Paresh , Doke Prakash Prabhakarrao , Gothankar Jayashree Sachin , Pore Prasad Dnyandeo , Palkar Sonali Hemant , Patil Archana Vasantrao , Deshpande Aniruddha Vinayakrao , Bhuyan Khanindra Kumar , Karnataki Madhusudan Vaman , Shrotri Aparna Nishikant
Published: August 2022

Background

Providing preconception care through healthcare workers at the primary health care level is a crucial intervention to reduce adverse pregnancy outcomes, consequently reducing neonatal mortality. Despite the availability of evidence, this window of opportunity remains unaddressed in many countries, including India. The public health care system is primarily accessed by rural and tribal Indian population. It is essential to know the frontline healthcare workers perception about preconception care. The study aimed to identify barriers and suggestions for framing appropriate strategies for implementing preconception care through primary health centers.

Methods

The authors conducted a qualitative study using focus group discussions (FGDs) with 45 healthcare workers in four FGDs (8–14 participants in each), in four blocks of Nashik district. The transcribed discussions were analyzed in MAXQDA software using the Socio-Ecological Model as an initial coding guide, including four levels of factors (individual, interpersonal, community, and institutional) that influenced an individual's behavior to use preconception care services.

Results

Healthcare workers had some knowledge about preconception care, limited to adolescent health and family planning services. The interpersonal factors included heavy workload, stress, lack of support and co-operation, and paucity of appreciation, and motivation. The perceived community factors included poverty, migration, poor knowledge of preconception care, lack of felt need for preconception services, the influence of older women in the household decision, low male involvement, myths and misconceptions regarding preconception services. The identified institutional factors were lack of human resources, specialized services, logistics, and challenges in delivering adolescent health and family planning programs. Healthcare workers suggested the need for program-specific guidelines, training and capacity building of human resources, an un-interrupted supply of logistics, and a unique community awareness drive supporting preconception care services.

Conclusion

Multi-level factors of the Socio-Ecological Model influencing the preconception care services should be considered for framing strategies in the implementation of comprehensive preconception care as a part of a continuum of care for life cycle phases of women.
Digital Economy and Health: A Case Study of a Leading Enterprise's Value Mining Mode in the Global Big Health Market
Authors: Guan Jialin , Xu Huijuan , Wang Yunfeng , Ma Yaoguang , Wang Yu , Gao Rui , Yu Kexuan
Published: August 2022

Coronavirus disease 2019 (COVID-19) swept across the world and posed a serious threat to human health. Health and elderly care enterprises are committed to continuously improving people's health. With the rapid development of the digital economy, many enterprises have established digital product-service ecosystems after combining “Internet +,” big data, cloud computing, and the big health industry. This paper uses the case study method to analyze the overseas market value mining mode of health and elderly care enterprises through in-depth research on leading health and elderly care enterprises. This study explores the value mining mode of the leading enterprise's global big health market using a cluster analysis and Bayesian model with the support of data on geographical characteristics, users' sleep habits, and national big health. This paper theoretically summarizes the successful cases of health and elderly care enterprises through digital transformation, which provides a useful reference for the intelligent transformation of the health and elderly care industry.
Association of body composition with pubertal timing in children and adolescents from Guangzhou, China
Authors: Zheng Yijin , Liang Jianping , Zeng Ding , Tan Weiqing , Yang Lun , Lu Shuang , Yao Wanwen , Yang Yi , Liu Li
Published: August 2022

Objective

An ongoing debate surrounds the relationship between body composition and pubertal timing, in particular for boys. This cross-sectional study aimed to investigate the association of body composition with pubertal timing among children and adolescents.

Methods

A total of 1,493 boys and 1,261 girls who entered puberty were enrolled in Guangzhou, China. Tanner stages were evaluated by examination of breast development for girls and testicular volume for boys. Fat mass (FM) and fat-free mass (FFM) were determined by bioelectrical impedance analysis. Parameters for body composition were transformed into age-and gender-specific Z-scores. The association of body composition with pubertal timing was examined using multinomial logistic regression with inverse probability weighting (IPW) based on the propensity score.

Results

For boys, IPW analysis showed Z-scores of body fat percentage (BF%) and FM index (FMI) were negatively associated with early puberty (OR = 0.75, 95% CI = 0.64–0.87; OR = 0.74, 95% CI = 0.63–0.88). As for girls, in contrast to boys, positive associations were seen between BF% and FMI with early puberty (OR = 1.39, 95% CI = 1.19–1.64; OR = 1.59, 95% CI = 1.33–1.90). With respect to appendicular skeletal muscle mass index (ASMI), there was a positive association with early puberty and a negative one with late puberty in boys (OR = 1.26, 95% CI = 1.07–1.49; OR = 0.82, 95% CI = 0.69–0.99).

Conclusion

There is a positive association of FM with early puberty for girls while negative for boys. FFM yields a positive association with early puberty and a negative one with late puberty in boys, but not in girls. Our findings highlight the gender differences in the connection between body composition and pubertal onset.
Sacubitril-valsartan for the treatment of hypertension in China: A cost-utility analysis based on meta-analysis of randomized controlled trials
Authors: Lou Yake , Yu Ying , Liu Jinxing , Huang Jing
Published: August 2022

Background

Sacubitril-valsartan was recommended for heart failure (HF) and proven cost-effective in HF. Recently, sacubitril-valsartan has been recommended to treat hypertension by the Chinese expert consensus. The cost utility of sacubitril-valsartan for hypertension remains uninvestigated.

Methods

A meta-analysis of randomized controlled trials (RCTs) was performed to investigate the real efficacy of sacubitril-valsartan on blood pressure, compared with angiotensin receptor blockers or placebo. A lifetime Markov model was developed to compare the cost utility of sacubitril-valsartan vs. valsartan. The primary outcome was the incremental cost-utility ratio (ICUR), representing the ratio of incremental costs to the incremental utility. The willingness-to-pay (WTP) threshold was three times of per capita gross domestic product (GDP) in China in 2021. Sacubitril-valsartan was considered cost-effective if the ICUR obtained was lower than the WTP threshold, otherwise, sacubitril-valsartanis was not cost-effective.

Results

A total of 10 RCTs of 5,781 patients were included in the meta-analysis. For comparison of sacubitril-valsartan 400 mg/day vs. valsartan 320 mg/day, a reduction in blood pressure (BP) of −5.97 (−6.38, −5.56) (p < 0.01) was observed. Cost-utility analysis showed that for a 60-year-old patient with hypertension, if sacubitril-valsartan was prescribed as the antihypertensive agent, he had a life expectancy of 11.91 quality-adjusted life-years (QALYs) with costs of 65,066 CNY, and if valsartan was prescribed as the antihypertensive agent, the life expectancy would be 11.82 QALY with costs of 54,769 CNY; thus, an ICUR of 108,622 CNY/QALY was obtained, lower than the WTP threshold.

Conclusion

Compared with valsartan, sacubitril-valsartan is more effective in reducing blood pressure and may result in more quality-adjusted life-year, although with higher costs. Sacubitril-valsartan is cost-effective for hypertension in the current China setting under the willingness-to-pay threshold of 3 times of per capita GDP.
Laboratory and clinical teaching experience of nursing professors in the COVID-19 pandemic era: Now and the future
Authors: Choi Seung-Yi , Jin Songxian , Kim Jung-Hee
Published: August 2022

Nursing professors must constantly interact with students, maintain a high level of professional performance, and meet targets and deadlines, even during a pandemic. Considering the changing educational environment, it is essential to identify contemporary limitations and problems to provide feedback for improvement. This study aimed to explore the laboratory and clinical teaching experiences of nursing professors during the COVID-19 pandemic. Focus group interviews were conducted with professors from the nursing departments of universities in Korea. In total, 19 professors who had laboratory and clinical experience participated in this study. The collected data were analyzed using thematic analysis. The analysis identified four themes. The themes included feeling helpless in the infection management system, uncertainty about the effectiveness of alternative practice training, acceptance of changes, and preparation for future practice training. As the necessity and possibility of non-face-to-face education have been confirmed by the pandemic, it is expected that classes using technology will be actively developed in nursing practice education. The roles and attitudes of teachers and educational institutions also need to change. Nursing professors should reflect upon and evaluate challenges to prepare for post-pandemic practical education.
Association between trace metals exposure and hearing loss
Authors: Zou Peixi , Li Menghuan , Chen Wei , Ji Junfeng , Xue Fei , Wang Zhiyi , Xu Li , Cheng You
Published: August 2022

Background

Trace metals have side-effect on human health. The association between trace metals exposure and hearing loss remains unclear.

Methods

A total of 8,128 participants were exacted for analysis of association between trace metals and hearing loss from the database of the National Health and Nutrition Examination Survey (NHANES) (2013–2018). Multivariable logistic regression and restricted cubic spline models were used to examine the association between trace metals and hearing loss.

Results

Participants with hearing loss had a higher level of lead, cadmium, molybdenum, tin, thallium, and tungsten (all p < 0.05). After adjusting for confounders, compared with the reference of the lowest quartile, the ORs with 95%CIs for hearing loss across quartiles were 1.14 (0.86, 1.51), 1.49 (1.12, 1.98), 1.32 (0.97, 1.80) for cobalt, and 1.35 (0.98, 1.87), 1.58 (1.15, 2.16), 1.75 (1.28, 2.40) for tin. Individuals with the level of cobalt at third quartile had 49% higher risks of hearing loss than those at lowest quartile. And participants with highest quartile of tin had 1.75-folds risks of hearing loss than those with lowest quartile of tin. There were increasing trends in risks of hearing loss with a raised level of thallium (p for trend <0.05). Restricted cubic spline regression analysis indicated that there was a nonlinear association between hearing loss and the levels of tin (p for nonlinearity = 0.021). Subgroup analysis showed that individuals of female, without hypertension and diabetes, and with a higher level of low-density lipoprotein cholesterol had modified effects on the associations between hearing loss and exposure to tin.

Conclusions

Our study indicated that exposure to cobalt and tin were significantly associated with hearing loss.
Strategies for the implementation of a nasal decolonization intervention to prevent surgical site infections within the Veterans Health Administration
Authors: Hockett Sherlock Stacey , Goedken Cassie Cunningham , Balkenende Erin C. , Dukes Kimberly C. , Perencevich Eli N. , Reisinger Heather Schacht , Forrest Graeme N. , Pfeiffer Christopher D. , West Katelyn A. , Schweizer Marin
Published: August 2022

As part of a multicenter evidence-based intervention for surgical site infection prevention, a qualitative study was conducted with infection control teams and surgical staff members at three Department of Veterans Affairs Healthcare Systems in the USA. This study aimed to identify strategies used by nurses and other facility champions for the implementation of a nasal decolonization intervention. Site visit observations and field notes provided contextual information. Interview data were analyzed with inductive and deductive content analysis. Interview data was mapped to the Expert Recommendations for Implementing Change (ERIC) compilation of implementation strategies. These strategies were then considered in the context of power and relationships as factors that influence implementation. We found that implementation of this evidence-based surgical site infection prevention intervention was successful when nurse champions drove the day-to-day implementation. Nurse champions sustained implementation strategies through all phases of implementation. Findings also suggest that nurse champions leveraged the influence of their role as champion along with their understanding of social networks and relationships to help achieve implementation success. Nurse champions consciously used multiple overlapping and iterative implementation strategies, adapting and tailoring strategies to stakeholders and settings. Commonly used implementation categories included: “train and educate stakeholders,” “use evaluative and iterative strategies,” “adapt and tailor to context,” and “develop stakeholder interrelationships.” Future research should examine the social networks for evidence-based interventions by asking specifically about relationships and power dynamics within healthcare organizations. Implementation of evidence-based interventions should consider if the tasks expected of a nurse champion fit the level of influence or power held by the champion.

Trial registration

ClinicalTrials.gov, identifier: NCT02216227.
Investigating routine care non-pharmacological treatment for adolescents with ADHD
Authors: Sibley Margaret H. , Reyes Francisco Juan Carlos , Rios-Davis Alexandria , Graziano Paulo A.
Published: August 2022

Objective

To characterize routine non-pharmacological care for youth with ADHD.

Methods

76 audio-recorded work-samples were collected from community mental health therapists in a large metropolitan area in the United States and were analyzed for operationally defined practice elements commonly included in evidence-based non-pharmacological treatment for ADHD. Analyses characterized community provider practices and examined predictors of using evidence-based (vs.low-value) practices.

Results

Individually delivered social skills training was the most commonly detected practice element (31.6% of practice samples). Parent involvement in routine care was uncommon (53.9% of sessions had no parental presence). Core elements of evidence-based practices were rarely delivered (e.g., organization skills training: 18.4% of tapes; operant reinforcement: 13.2%); when evidence-based content was introduced, it was typically implemented at a very low intensity. Patient and provider characteristics did not predict use of evidence-based practices.

Conclusions

Routine non-pharmacological care for adolescent ADHD primarily consisted of low value practices such as youth-directed treatment and social skills training with low parent involvement and only occasional therapy homework. To improve quality of care, efforts to de-implement low value practices should be coupled with efforts to implement evidence-based practices (i.e., parent involvement, measurement-based care, organization skills training, use of operant reinforcement).
Nuts and bolts of tropical tuna purse seine nets recycling: A circular business model
Authors: Andrés Marga , Zudaire Iker , Larreta Joana , Asueta Asier , González Nekane , Molist Marta , Uribesalgo Edu , Basurko Oihane C.
Published: August 2022

Tropical tuna purse seiners are one of the most important contributors to end-of-life (EoL) fishing gears in the world, and these fishing nets can become a promising secondary raw material. Thus, tuna companies are looking for possibilities to valorize them by applying circular economy (CE). This contribution aims at assessing the viability of creating a circular business model out of recycled tropical tuna purse seine EoL nets. The yearly contribution of the Spanish tuna freezer purse seine companies to EoL fishing nets was estimated at 900 tons. Three pilot projects were implemented (involving 80 tons of EoL tuna nets) to learn about the monetary and material flows, supply chain, stakeholders' perception, and the environmental impacts of upcycling polyamide nets into four marketable products (i.e., conditioned fishing nets, backpacks, fishers' dungarees, and sunglasses). The results indicate that recycled regrinds/flakes and pellets were 37 and 50%, respectively, more expensive than virgin counterparts, but the yarn may achieve competitive production costs in the textile industry, with an additional environmental benefit close to 69% per kg of virgin–origin yarn. The challenges faced when recycling EoL polyamide fishing nets were discussed. Innovation and logistics appear to play a fundamental role in making the business sustainable. Besides, the circular business model methodology to assess the value proposition was also discussed in its empirical application.
Postoperative antibiotics and infection rates after implant-based breast reconstruction: A systematic review and meta-analysis
Authors: Hu Yang , Zhou Xuan , Tong Xiaofei , Chen Xiangyu , Wang Mingzhu , Wu Xianrui , Li Peiting , Tang Fengjie , Zhou Jianda , Li Ping
Published: August 2022

Purpose

Infection is the most common complication following breast implant surgery. Nevertheless, the systematic administration of antibiotics after breast implant surgery has been subjected to controversial debate. In this study, we sought to elucidate the association between infection and the use of antibiotics as an aftermath of breast implantation surgical procedures.

Methods

Relevant studies were identified from PubMed, Web of Science, and EMBASE search mining. The extracted data included study type, basic characteristics, administrated antibiotic information, and clinical outcomes. Random-effects models were utilized to estimate outcomes, while study quality, statistical bias, and heterogeneity were also analyzed.

Results

A total of 7 studies involving a total of 9,147 subjects were included. The results demonstrated that the use of antibiotics after breast implantation reduced the incidence of infection (risk ratio [RR]: 0.65, 95% CI, 0.46–0.90). Nevertheless, smoking, obesity and diabetes type II are risk factors for postoperative infections. Sensitivity analysis verified the robustness of the results.

Conclusions

Our study identified the administration of antibiotics after breast implantation as an intervention that decreased the incidence of infection. Smoking, obesity, and diabetes type II are risk factors for postoperative infections. These findings strongly suggest that timely and effective antibiotic interventions will be crucial in future clinical practice, which may reduce the risk of postoperative infection following breast implantation.
Aggressive angiomyxoma: The first case report in skull
Authors: Cao Zexin , Miao Lifeng , Liu Min , Liu Wenyu , Zhang Hengrui , Liu Xuchen , Wang Jiwei , Wang Xinyu
Published: August 2022

Aggressive angiomyxoma (AAM) is a rare mesenchymal tumor primarily growing in the soft tissue of the pelvis and perineum in women of reproductive age. It is a benign tumor that still has a probability of being accompanied by localized invasion. Although negative margins of resection are difficult to achieve due to the invasive nature of the tumor and the lack of a well-defined capsule, the first line of treatment for AAM is surgery. The diagnosis of AAM is difficult to make due to a lack of specific manifestations and specific tumor markers. In this study, we reported a case of aggressive angiomyxoma in a 2-year-old girl that rarely develops in the skull with craniocerebral compression. The patient initially had a mass on her head that attracted the attention of her family, and then she began to have episodic headaches. Surgery was performed after hospitalization, and the tumor recurred 1 year after the operation, around the originally affected skull.
TIMM13 as a prognostic biomarker and associated with immune infiltration in skin cutaneous melanoma (SKCM)
Authors: Zhou Sitong , Han Yuanyuan , Yang Ronghua , Pi Xiaobing , Li Jiehua
Published: August 2022

Objective

Providing protection against aggregation and guiding hydrophobic precursors through the mitochondria’s intermembrane space, this protein functions as a chaperone-like protein. SLC25A12 is imported by TIMM8 as a result of its interaction with TIMM13. In spite of this, it is still unknown how TIMM13 interacts with skin cutaneous melanoma (SKCM) and tumor-infiltrating lymphocytes (TILs).

Methods

Aberrant expression of TIMM13 in SKCM and its clinical outcome was evaluated with the help of multiple databases, including the Xiantao tool (https://www.xiantao.love/), HPA, and UALCAN. TISIDB and Tumor Immune Estimation Resources (TIMER) databases were applied to explore the association between TIMM13 and tumor infiltration immune cells. OS nomogram was constructed, and model performance was examined. Finally, TIMM13 protein expression was validated by immunohistochemistry (IHC).

Results

TIMM13 expression was higher in SKCM samples than in peritumor samples. TIMM13 was strongly associated with sample type, subgroup, cancer stage, lymph node stage, and worse survival. Further, upregulation of TIMM13 was significantly associated with immunoregulators, and chemokines, as well as T cells, B cells, monocytes, neutrophils, macrophages, and T-cell regulators. An analysis of bioinformatic data uncovered that TIMM13 expression was strongly associated with PD1 (T-cell exhaustion marker). The nomogram showed good predictive performance based on calibration plot. TIMM13 was highly expressed in melanoma tissue samples than in normal samples.

Conclusion

In brief, TIMM13 may be a prognostic biomarker for SKCM. It might modulate the tumor immune microenvironment and lead to a poorer prognosis. In addition, it is necessary to study the targeted therapy of TIMM13.
Mid-Infrared Imaging Characterization to Differentiate Lung Cancer Subtypes
Authors: Kontsek E. , Pesti A. , Slezsák J. , Gordon P. , Tornóczki T. , Smuk G. , Gergely S. , Kiss A.
Published: August 2022

Introduction: Lung cancer is the most common malignancy worldwide. Squamous cell carcinoma (SQ) and adenocarcinoma (LUAD) are the two most frequent histological subtypes. Small cell carcinoma (SCLC) subtype has the worst prognosis. Differential diagnosis is essential for proper oncological treatment. Life science associated mid- and near-infrared based microscopic techniques have been developed exponentially, especially in the past decade. Vibrational spectroscopy is a potential non-destructive approach to investigate malignancies.

Aims: Our goal was to differentiate lung cancer subtypes by their label-free mid-infrared spectra using supervised multivariate analyses.

Material and Methods: Formalin-fixed paraffin-embedded (FFPE) samples were selected from the archives. Three subtypes were selected for each group: 10-10 cases SQ, LUAD and SCLC. 2 μm thick sections were cut and laid on aluminium coated glass slides. Transflection optical setup was applied on Perkin-Elmer infrared microscope. 250 × 600 μm areas were imaged and the so-called mid-infrared fingerprint region (1800-648cm−1) was further analysed with linear discriminant analysis (LDA) and support vector machine (SVM) methods.

Results: Both “patient-based” and “pixel-based” approaches were examined. Patient-based analysis by using 3 LDA models and 2 SVM models resulted in different separations. The higher the cut-off value the lower is the accuracy. The linear C-support vector classification (C-SVC) SVM resulted in the best (100%) accuracy for the three subtypes using a 50% cut-off value. The pixel-based analysis gave, similarly, the linear C-SVC SVM model to be the most efficient in the statistical indicators (SQ sensitivity 81.65%, LUAD sensitivity 82.89% and SCLC sensitivity 88.89%). The spectra cut-off, the kernel function and the algorithm function influence the accuracy.

Conclusion: Mid-Infrared imaging could be used to differentiate FFPE lung cancer subtypes. Supervised multivariate tools are promising to accurately separate lung tumor subtypes. The long-term perspective is to develop a spectroscopy-based diagnostic tool, revolutionizing medical differential diagnostics, especially cancer identification.
Discrete-Time Observations of Brownian Motion on Lie Groups and Homogeneous Spaces: Sampling and Metric Estimation
Authors: Jensen Mathias Højgaard , Joshi Sarang , Sommer Stefan
Published: August 2022

We present schemes for simulating Brownian bridges on complete and connected Lie groups and homogeneous spaces. We use this to construct an estimation scheme for recovering an unknown left- or right-invariant Riemannian metric on the Lie group from samples. We subsequently show how pushing forward the distributions generated by Brownian motions on the group results in distributions on homogeneous spaces that exhibit a non-trivial covariance structure. The pushforward measure gives rise to new non-parametric families of distributions on commonly occurring spaces such as spheres and symmetric positive tensors. We extend the estimation scheme to fit these distributions to homogeneous space-valued data. We demonstrate both the simulation schemes and estimation procedures on Lie groups and homogenous spaces, including SPD(3)=GL+(3)/SO(3) and S2=SO(3)/SO(2).
Numerical Simulation and Torsional Vibration Mitigation of Spatial Eccentric Structures with Multiple Magnetorheological Dampers
Authors: Yang Yang , Guo Ying-Qing
Published: August 2022

Eccentric structures will have torsional vibrations subjected to earthquakes, which can accelerate the damage of structures, and even become the main cause of building collapse. Semi-active control systems equipped with multiple magnetorheological (MR) dampers have been widely applied in structural vibration control. In this study, numerical models of spatial eccentric structures with multiple MR dampers were established, and time history analysis was conducted to mitigate torsional vibrations of eccentric structures. Firstly, a full-scale spatial eccentric structure model with both plan asymmetry and vertical irregularity was established in OpenSEES, and the accuracy of the structure model was verified by comparisons with model results from SAP2000. Then, the mathematical model of MR dampers was introduced to the structure model using the ‘Truss’ element and self-defined material in OpenSEES, and damping forces obtained from the MR damper model were compared with experimental data. Finally, modal analysis and nonlinear time history analysis of the eccentric structure model equipped with multiple MR dampers subjected to different seismic excitations were performed. Comparisons between the seismic responses of the uncontrolled structure and the structure with multiple MR dampers were carried out to demonstrate the effectiveness of the MR control system. Numerical results show that the control system with multiple MR dampers can significantly attenuate the torsional vibrations of eccentric structures, and thus possess significant engineering application prospects.
Semantic Segmentation Algorithm of Rice Small Target Based on Deep Learning
Authors: Li Shuofeng , Li Bing , Li Jin , Liu Bin , Li Xin
Published: August 2022

At present, rice is generally in a state of dense adhesion and small granular volume during processing, resulting in no effective semantic segmentation method for rice to extract complete rice. Aiming at the above problems, this paper designs a small object semantic segmentation network model based on multi-view feature fusion. The overall structure of the network is divided into a multi-view feature extraction module, a super-resolution feature building module and a semantic segmentation module. The extraction ability of small target features is improved by super-resolution construction of small target detail features, and the learning ability of the network for small target features is enhanced and expanded through multi-view. At the same time, a dataset of quality inspection during rice processing was constructed. We train and test the model on this dataset. The results show that the average segmentation accuracy of the semantic segmentation model in this paper reaches 87.89%. Compared with the semantic segmentation models such as SegNet, CBAM, RefineNet, DeepLabv3+ and G-FRNet, it has obvious advantages in various indicators, which can provide rice quality detection and an efficient method of rice grain extraction.
The Effect of Appearance of Virtual Agents in Human-Agent Negotiation
Authors: Türkgeldi Berkay , Özden Cana Su , Aydoğan Reyhan
Published: August 2022

Artificial Intelligence (AI) changed our world in various ways. People start to interact with a variety of intelligent systems frequently. As the interaction between human and AI systems increases day by day, the factors influencing their communication have become more and more important, especially in the field of human-agent negotiation. In this study, our aim is to investigate the effect of knowing your negotiation partner (i.e., opponent) with limited knowledge, particularly the effect of familiarity with the opponent during human-agent negotiation so that we can design more effective negotiation systems. As far as we are aware, this is the first study investigating this research question in human-agent negotiation settings. Accordingly, we present a human-agent negotiation framework and conduct a user experiment in which participants negotiate with an avatar whose appearance and voice are a replica of a celebrity of their choice and with an avatar whose appearance and voice are not familiar. The results of the within-subject design experiment show that human participants tend to be more collaborative when their opponent is a celebrity avatar towards whom they have a positive feeling rather than a non-celebrity avatar.
Quantitation and Identification of Therapeutic Anti-CD22 Monoclonal Antibodies in a Cell-Based ELISA Method
Authors: Fu Shengyu , Zhao Qi
Published: August 2022

Since they lack native soluble membrane antigens, the analysis and selection of antigen-specific antibodies are commonly performed on whole live cells. Here, we have developed a simple and convenient enzyme-linked immunosorbent assay (ELISA) based on cell membrane antigens. Soluble cell membrane proteins isolated from Raji cells were immobilized on the polystyrene microplate, which permitted the assessment of a therapeutic anti-CD22 monoclonal antibody. The experiments showed less variability in the intra-assay. Compared to the living cell ELISAs, the advantage of the assay is avoiding cell losses and high variation of optical density (OD) readings. We provide a quantitative and reproducible ELISA that can be potentially applied to the development of specific antibodies against cell surface antigens.
Different European Perspectives on the Treatment of Clinical Mastitis in Lactation
Authors: Preine Franziska , Herrera Demetrio , Scherpenzeel Christian , Kalmus Piret , McCoy Finola , Smulski Sebastian , Rajala-Schultz Päivi , Schmenger Anne , Moroni Paolo , Krömker Volker
Published: August 2022

As part of the European Network for Optimization of Veterinary Antimicrobial Treatment (ENOVAT), a webinar on the topic “Mastitis Treatment in Lactation” was held, in which eight mastitis experts from different European countries (Spain, The Netherlands, Estonia, Ireland, Poland, Finland, Germany, and Italy) presented their treatment approaches for clinical mastitis in lactation. The aim of this study was to compare the therapeutic approaches to identify commonalities and differences. In all eight participating countries, the decision to start treatment is usually made by the veterinarians, while the farm personnel are responsible for treatment administration. Antibiotic treatment is then typically administered intramammarily. The treatment duration often depends on the label instructions and is frequently extended if Staphylococcus aureus or Streptococcus uberis is involved. Administering supportive therapy, especially non-steroidal anti-inflammatory drugs (NSAIDs) is an established practice in all countries. Penicillin is the first-choice drug for the treatment of mastitis in an increasing number of countries. The use of critically important antimicrobials (CIAs) such as quinolones and third- and fourth-generation cephalosporins is at a low level in Finland and The Netherlands. In Estonia, Germany, Italy, and Spain, the use of CIAs is declining and is only allowed if milk samples are analyzed in advance following the legal framework. Systems for monitoring antibiotic use are being introduced in more and more countries. This exchange of different views will help the European countries to move towards a common high standard of antimicrobial stewardship in veterinary medicine.
Characterization of Carbapenem-Resistant K. Pneumoniae Isolated from Intensive Care Units of Zagazig University Hospitals
Authors: Gandor Nessma Hessin Mohamed , Amr Ghada EL-Sayed , Eldin Algammal Sahar Mohamed Saad , Ahmed Alshymaa Abdullah
Published: August 2022

The advent of carbapenem-resistant Klebsiella pneumoniae (CRKP) poses a significant challenge to public health, as carbapenems are typically employed as a last resort to treat nosocomial infections caused by such organisms, especially in intensive care units (ICUs). This study aims to characterize the CRKP isolated from patients admitted to the Zagazig University Hospitals (ZUHs) ICU in Egypt. About 56.2%, 41.0%, and 32.4% of the isolates indicated the presence of blaNDM, blaOXA-48, and blaKPC, respectively. Carbapenemase-encoding genes were found in many isolates, and blaNDM was the most predominant gene. Nevertheless, this situation has become a heavy burden in developing countries, including Egypt, and is associated with substantial morbidity, mortality, and increased healthcare expenses.
Phage-Related Ribosomal Proteases (Prps): Discovery, Bioinformatics, and Structural Analysis
Authors: Hotinger Julia A. , Gallagher Allison Hannah , May Aaron E.
Published: August 2022

Many new antimicrobials are analogs of existing drugs, sharing the same targets and mechanisms of action. New antibiotic targets are critically needed to combat the growing threat of antimicrobial-resistant bacteria. Phage-related ribosomal proteases (Prps) are a recently structurally characterized antibiotic target found in pathogens such as Staphylococcus aureus, Clostridioides difficile, and Streptococcus pneumoniae. These bacteria encode an N-terminal extension on their ribosomal protein L27 that is not present in other bacteria. The cleavage of this N-terminal extension from L27 by Prp is necessary to create a functional ribosome. Thus, Prp inhibition may serve as an alternative to direct binding and inhibition of the ribosome. This bioinformatic and structural analysis covers the discovery, function, and structural characteristics of known Prps. This information will be helpful in future endeavors to design selective therapeutics targeting the Prps of important pathogens.
Salmonella enterica Serovar Dublin from Cattle in California from 1993–2019: Antimicrobial Resistance Trends of Clinical Relevance
Authors: Fritz Heather M. , Pereira Richard V. , Toohey-Kurth Kathy , Marshall Edie , Tucker Jenna , Clothier Kristin A.
Published: August 2022

Salmonella enterica subsp. enterica serovar Dublin (S.Dublin) is a cattle-adapted pathogen that has emerged as one of the most commonly isolated and multidrug resistant (MDR) serovars in cattle. S.Dublin may be shed in feces, milk, and colostrum and persist in asymptomatic cattle, leading to spread and outbreaks in herds. Though infections with S.Dublin in humans are rare, they are frequently severe, with extraintestinal spread that requires hospitalization and antimicrobial therapy. To determine minimum inhibitory concentration (MIC) and antimicrobial resistance (AMR) patterns and trends in cattle in California, broth microdilution testing was performed on 247 clinical S. Dublin isolates recovered from cattle at the California Animal Health and Food Safety Laboratory System (CAHFS) over the last three decades (1993–2019). Mean MICs and classification of resistance to antimicrobial drugs using a clinical livestock panel and the National Antimicrobial Resistance Monitoring System (NARMS) Gram-negative drug panels were utilized to assess prevalence and trends in AMR. Findings indicate an increase in AMR for the years 1993 to 2015. Notably, compared to the baseline year interval (1993–1999), there was an increase in resistance among quinolone and cephalosporin drugs, as well as an increased number of isolates with an MDR profile.
A Caveat When Using Alkyl Halides as Tagging Agents to Detect/Quantify Reactive Sulfur Species
Authors: Wu Xiaohua , Xin Yuping , Wang Qingda , Xia Yongzhen , Xun Luying , Liu Huaiwei
Published: August 2022

Using alkyl halides to tag reactive sulfur species (RSSs) (H2S, per/polysulfide, and protein-SSH) is an extensively applied approach. The underlying supposition is that, as with thiols, RSS reacts with alkyl halides via a nucleophilic substitution reaction. We found that this supposition is facing a challenge. RSS also initiates a reductive dehalogenation reaction, which generates the reduced unloaded tag and oxidized RSS. Therefore, RSS content in bio-samples might be underestimated, and its species might not be precisely determined when using alkyl halide agents for its analysis. To calculate to the extent of this underestimation, further studies are still required.
Pre-Treatment Physical Activity Could Positively Influence Pregnancy Rates in IVF despite the Induced Oxidative Stress: A Cohort Study on Salivary 8-Hydroxy-2′-deoxyguanosine
Authors: Prémusz Viktória , Lendvai-Emmert Dominika , Makai Alexandra , Amrein Krisztina , Chauhan Shalini , Bódis József , Kovács Kálmán András , Várnagy Ákos
Published: August 2022

(1) Background: This study was designed to define whether pretreatment habitual physical activity (PA)-induced oxidative stress (OS) influences outcome measures by using 8-hydroxy-2′-deoxyguanosine (8-OHdG) in saliva samples of patients undergoing in vitro fertilization (IVF). (2) Method: In this cohort study, samples were obtained from 26 patients (age: 34.6 ± 5.5 years, BMI: 25.3 ± 5.1, infertility: 51.0 ± 28.7 months) before the treatment and a follow-up of outcome measures of IVF/ICSI. The 8-OHdG was evaluated by Abcam’s ELISA (ab201734), PA patterns by GPAQ-H and ActiGraph GT3X; (3) Results: The number of matured oocytes was positively influenced by the GPAQ-H recreation MET (R2 = 0.367, F = 10.994, p = 0.004; β = 0.005, p = 0.004, B Constant = 4.604) and a positive significant relationship (R2 = 0.757, F = 17.692, p < 0.001, B Constant = 1.342) was found with GPAQ-H recreational PA MET (β = 0.004, p < 0.001), and Grade 1 embryos and higher very vigorous activity (GT3X) were accompanied (R2 = 0.958, F = 408.479, p < 0.001) by higher ß-hCG levels (β = 63.703, p ≤ 0.001). Unanticipated positive correlation between 8-OHdG and ß-hCG level (R = 0.467, p = 0.028) was noticed, and there were significant differences in 8-OHdG in biochemical pregnancies (pregnant: 54.82 ± 35.56 ng/mL, non-pregnant: 30.06 ± 10.40 ng/mL, p = 0.022) as well. (4) Conclusions: Pretreatment PA could positively influence reproductive performance in IVF/ICSI despite the induced OS. However, a more sensitive biomarker and the recommended amount of activity should be further investigated.
Plasma CoQ10 Status in Patients with Propionic Acidaemia and Possible Benefit of Treatment with Ubiquinol
Authors: Stanescu Sinziana , Belanger-Quintana Amaya , Fernández-Felix Borja Manuel , Ruiz-Sala Pedro , Alcaide Patricia , Arrieta Francisco , Martínez-Pardo Mercedes
Published: August 2022

Propionic acidaemia (PA) is an innate error of metabolism involving a deficiency in the enzyme propionyl-CoA carboxylase. Better control of acute decompensation episodes together with better treatment and monitoring have improved the prognosis of patients with this problem. However, long-term complications can arise in those in whom good metabolic control is achieved, the result of mitochondrial dysfunction caused by deficient anaplerosis, increased oxidative stress, and reduced antioxidative capacity. Coenzyme Q10 (CoQ10) is a nutritional supplement that has a notable antioxidative effect and has been shown to improve mitochondrial function. The present prospective, interventional study examines the plasma concentration of CoQ10 in patients with PA, their tolerance of such supplementation with ubiquinol, and its benefits. Seven patients with PA (aged 2.5 to 20 years, 4 males) received supplements of CoQ10 in the form of ubiquinol (10 mg/kg/day for 6 months). A total of 6/7 patients showed reduced plasma CoQ10 concentrations that normalized after supplementation with ubiquinol (p-value < 0.001), which was well tolerated. Urinary citrate levels markedly increased during the study (p-value: 0.001), together with elevation of citrate/methlycitrate ratio (p-value: 0.03). No other significant changes were seen in plasma or urine biomarkers of PA. PA patients showed a deficiency of plasma CoQ10, which supplementation with ubiquinol corrected. The urinary excretion of Krebs cycle intermediate citrate and the citrate/methylcitrate ratio significantly increased compared to the baseline, suggesting improvement in anaplerosis. This treatment was well tolerated and should be further investigated as a means of preventing the chronic complications associated with likely multifactorial mitochondrial dysfunction in PA.
Meta-Analysis and Systematic Review of the Association between a Hypoactive NCF1 Variant and Various Autoimmune Diseases
Authors: Zhang Liang , Wax Jacqueline , Huang Renliang , Petersen Frank , Yu Xinhua
Published: August 2022

Genetic association studies have discovered the GTF2I-NCF1 intergenic region as a strong susceptibility locus for multiple autoimmune disorders, with the missense mutation NCF1 rs201802880 as the causal polymorphism. In this work, we aimed to perform a comprehensive meta-analysis of the association of the GTF2I-NCF1 locus with various autoimmune diseases and to provide a systemic review on potential mechanisms underlying the effect of the causal NCF1 risk variants. The frequencies of the two most extensively investigated polymorphisms within the locus, GTF2I rs117026326 and NCF1 rs201802880, vary remarkably across the world, with the highest frequencies in East Asian populations. Meta-analysis showed that the GTF2I-NCF1 locus is significantly associated with primary Sjögren’s syndrome, systemic lupus erythematosus, systemic sclerosis, and neuromyelitis optica spectrum disorder. The causal NCF1 rs201802880 polymorphism leads to an amino acid substitution of p.Arg90His in the p47phox subunit of the phagocyte NADPH oxidase. The autoimmune disease risk His90 variant results in a reduced ROS production in phagocytes. Clinical and experimental evidence shows that the hypoactive His90 variant might contribute to the development of autoimmune disorders via multiple mechanisms, including impairing the clearance of apoptotic cells, regulating the mitochondria ROS-associated formation of neutrophil extracellular traps, promoting the activation and differentiation of autoreactive T cells, and enhancing type I IFN responses. In conclusion, the identification of the association of NCF1 with autoimmune disorders demonstrates that ROS is an essential regulator of immune tolerance and autoimmunity mediated disease manifestations.
Near Infrared Spectroscopy as a Traceability Tool to Monitor Black Soldier Fly Larvae (Hermetia illucens) Intended as Animal Feed
Authors: Alagappan Shanmugam , Hoffman Louwrens C. , Mantilla Sandra M. Olarte , Mikkelsen Deirdre , James Peter , Yarger Olympia , Cozzolino Daniel
Published: August 2022

The demand for animal proteins, especially from pork and poultry, is projected to increase significantly due to rapid growth in population and underlying socio-economic conditions. Livestock rearing using conventional feed ingredients is becoming challenging due to climate change and several other factors, thereby suggesting the need for alternative, viable and sustainable animal feed sources. The use of black soldier fly larvae (BSFL) (Hermetia illucens) as a component in animal feed is a promising candidate due to their ability to valorise different organic waste streams. The nutrient composition of BSFL reared on organic waste streams is also comparable to that of several conventional animal feed ingredients and varies depending upon the feed, rearing conditions, and the morphological stage of the larvae. The identification of organic waste is of importance as it can determine not only the composition but also the safety issues of BSFL as an animal feed ingredient. The objective of this study was to determine the ability of near-infrared (NIR) spectroscopy to trace the food waste used to grow BSFL. Samples of BSFL (5th and 6th instar BSFL; n = 50) obtained from a commercial production facility were analysed using NIR spectroscopy. Partial least squares discriminant analysis (PLS-DA) was employed to develop the models. The outcomes of this study revealed that NIR spectroscopy could distinguish different larval instars and suggested the importance of larval instars in developing calibration models for traceability applications. The developed PLS-DA model could predict the feed source used for rearing the 5th instar larvae (R2 value: 0.89) and 6th instar pre-pupae (R2 value: 0.91). This suggests that NIR spectroscopy could be used as a non-invasive traceability tool for BSFL and to assist in selecting the suitable time frame for larvae harvesting in commercial facilities.
Variable Rate Point Cloud Attribute Compression with Non-Local Attention Optimization
Authors: Huo Xiao , Zhang Saiping , Yang Fuzheng
Published: August 2022

Point clouds are widely used as representations of 3D objects and scenes in a number of applications, including virtual and mixed reality, autonomous driving, antiques reconstruction. To reduce the cost for transmitting and storing such data, this paper proposes an end-to-end learning-based point cloud attribute compression (PCAC) approach. The proposed network adopts a sparse convolution-based variational autoencoder (VAE) structure to compress the color attribute of point clouds. Considering the difficulty of stacked convolution operations in capturing long range dependencies, the attention mechanism is incorporated in which a non-local attention module is developed to capture the local and global correlations in both spatial and channel dimensions. Towards the practical application, an additional modulation network is offered to achieve the variable rate compression purpose in a single network, avoiding the memory cost of storing multiple networks for multiple bitrates. Our proposed method achieves state-of-the-art compression performance compared to other existing learning-based methods and further reduces the gap with the latest MPEG G-PCC reference software TMC13 version 14.