bluuebunny/crossref_metadata_2025 huggingface dataset:
Pengaruh Pursed Lip Breathing Terhadap Peningkatan Toleransi Aktivitas Pada Pasien Penyakit Paru Obstruktif Kronik (PPOK)
Authors: Andasari Ririn , Salihun Saleha , Prabaningtyas Ayu Rizki
Published: February 2025

Penyakit Paru Obstruksi Kronis (PPOK) adalah gangguan saluran pernafasan kronis yang tidak reversibel penuh yang disebabkan oleh obstruksi saluran nafas. Keluhan utama pasien PPOK adalah sesak nafas yang bersifat progresif, irreversible dan intoleransi aktivitas. Pasien PPOK aktivitas fisik bisa menjadi tantangan, terutama karena saluran napas yang menyempit. Namun, teknik Pursed lips breathing dapat membantu dalam meningkatkan toleransi aktivitas.  Pursed lips breathing adalah teknik pernapasan yang digunakan untuk membantu meningkatkan kapasitas paru dan mempermudah pernapasan pada orang yang mengalami kesulitan bernapas, seperti pada penyakit paru obstruktif kronis (PPOK).  Penelitian ini termasuk Pra Eksperimentaldenganpre test-post test one-group design yang bertujuan untuk mengetahui pengaruh Pursed Lip Breathing terhadap peningkatan toleransi aktivitas pada pasien PPOK di BBKPM Makassar.Sampel penelitian adalah pasien PPOKyang dipilih sesuai dengan kriteria inklusi, dan diperoleh sampel sebanyak 11 orang yangdiberikan latihanPursed Lip Breathing. Alat ukur yang digunakan untuk pengumpulan data adalah Modified Borg dengan mengukur derajat sesak. Dilihat dari nilai rerata menunjukkan adanya perubahan untuk tingkat toleransi aktivitas yaitu dari 4,00 sebelum diberikanPursed Lip Breathing menjadi 2,09 setelah diberikan Pursed Lip Breathing, yang berarti bahwa terjadi penurunan tingkat derajat sesak sehingga terjadi peningkatan toleransi aktivitas. Berdasarkan analisis uji Wilcoxon, diperoleh hasil p= 0,003 (p<0,05) yang berarti bahwa intervensi Pursed Lip Breathing dapat meningkatkan toleransi aktivitas pada penderita PPOK.. Dapat disimpulkan bahwa Pemberian Pursed Lip Breathing dapat memberikan pengaruh yang signifikan terhadappeningkatan toleransi aktivitas pada pasien PPOK
Book Review: Right Where We Belong: How Refugee Teachers and Students Are Changing the Future of Education by Sarah Dryden-Peterson
Authors: Darwish Farzanah
Published: February 2025

In Right Where We Belong: How Refugee Teachers and Students Are Changing the Future of Education, Sarah Dryden-Peterson draws from ethnographic case study research and in-depth interviews to offer a comprehensive analysis of the education challenges refugee youth face globally, as well as solutions for policymakers and practitioners. In her review of Dryden-Peterson's book, Farzanah Darwish highlights the power and potential of refugee-led education programs, which offer critical opportunities for both teachers and students in conflict-affected countries to drive local solutions. Darwish suggests that readers in the field of education in emergencies will be interested in the book's exploration of how innovative and flexible forms of education that are grounded in mental health support, community involvement, and culturally relevant curricula can support the unique needs of refugee communities.
Schools as Sites of Social Reproduction: Student Interactions in Diverse Secondary Schools in Nigeria
Authors: Salmon-Letelier Marlana , Russell S. Garnett
Published: February 2025

In this study, we explore intergroup relations and student interactions in eight diverse secondary schools in Nigeria over one academic year. We use mixed methods and a social network analysis of these interactions and relationships to highlight the perspectives of students within a divided society. We analyze data from student interviews we conducted and social network data from our student surveys to explore the ways students exhibit ethnic and religious relations in a school setting. This study finds that Hausa Muslims are the most segregated group within Federal Unity Colleges in Nigeria, driven by the intersection of religion, ethnicity, and language. Religion emerges as a stronger social boundary than ethnicity. Our findings also point to the importance of both academic and nonacademic spaces (such as dormitories, where students can separate into groups) in mediating student interactions. Our work contributes to the discourse in the fields of education, conflict, and peacebuilding and, more broadly, to discussions in comparative education about the role schools play in mitigating or exacerbating intergroup conflict.
Municipality Synthetic Gini Index for Colombia: A Machine Learning Approach
Authors: Riveros-Gavilanes John Michael
Published: February 2025

This paper presents two synthetic estimations of the Gini coefficient at a municipality level for Colombia in the years 2000-2020. The methodology relies on several machine learning models to select the best model for imputation of the data. This derives in two Random Forest models were the first is characterized by containing Dominant Fixed Effects, while the second contains a set of Dominant Varying Factors. Upon these estimations, the Synthetic Gini Coefficients for both models are inspected, and public links are generated to access them. The Dominant Fixed Effects models is rather ”stiff” in contrast to the Varying Factor model. Hence, for researchers it is recommended to use the Synthetic Gini Coefficient with Varying Factors because it contains greater variability across time than the Dominant Fixed Effects models.
Is There a Third Text in Zeina Hashem Beck’s Duets? Translingual Poetics in O
Authors: Hambuch Doris
Published: February 2025

This article traces Zeina Hashem Beck’s development of a new poetic form called the “duet.” Duets include equal parts written in two different languages, English and Arabic for the Lebanese poet whose recent collection, O, includes six such duets. The ideal bilingual reader brings sufficient context in both languages to the understanding of the dialogue between the two duet halves. This study argues, however, that access to the third text hidden in this dialogue is not exclusive to those equally familiar with the different represented languages. Close readings of “daily كل يوم” and “blue أزرق” identify the characteristics and variations of the new poetic form. Analysis of “prophecy نبوة” and “Ode to Babel نشيد الانتظار” centers around translingual poetics. Scrutiny of “Dear white critic, رفيقي في الرحيل,” and “Ode to Leaving غربة” highlights the impact of projected audiences on the duet development. Relying on a comparative analysis of these six examples, the article discusses the benefits of ever improving machine translation, the availability of native speaker consultations, as well as a growing polyglot imaginary as extensions of traditional tools conducive to poetry exegesis.
Pengaruh Terapi Spiritual Emotional Freedom Technique (Seft) Terhadap Pengurangan Nyeri Dismenore Pada Remaja
Authors: Rahmana Mochammad Faizal Rahmana , Fatmawati Ariani , Imansari Bhekti
Published: February 2025

Dismenore merupakan nyeri perut saat menstruasi yang dapat menghambat aktivitas, seperti sulit konsentrasi dalam belajar dan tidak masuk sekolah. Angka kejadian dismenore pada remaja cukup tinggi. Salah satu metode non-farmakologis untuk mengatasi masalah ini adalah Terapi Spiritual Emotional Freedom Technique (SEFT). Penelitian ini bertujuan untuk menilai efektivitas terapi SEFT dalam mengurangi nyeri dismenore pada remaja. Pendekatan yang digunakan adalah quasi-eksperimen dengan desain pre-test post-test dengan kelompok kontrol. Jumlah sampel sebanyak 54 responden dibagi rata ke dalam kelompok intervensi dan kelompok kontrol. Kriteria inklusi meliputi siswi yang sudah menstruasi, mengalami dismenore dengan intensitas nyeri ringan sampai sedang, tidak mengonsumsi analgesik, dan tidak menjalani terapi non-farmakologis. Kriteria eksklusi mencakup memiliki cedera abdomen, perdarahan hebat saat menstruasi, dan riwayat gangguan kandungan seperti endometriosis, radang panggul, kista, atau tumor. Intensitas nyeri diukur menggunakan Numeric Rating Scale (NRS). Analisis data dilakukan secara univariat untuk mengidentifikasi distribusi frekuensi, dan analisis bivariat menggunakan uji Mann-Whitney. Hasil penelitian menunjukkan adanya penurunan signifikan pada intensitas nyeri di kelompok intervensi setelah mendapatkan terapi SEFT dibandingkan kelompok kontrol. Penelitian ini mengindikasikan bahwa terapi SEFT efektif dalam mengurangi rasa nyeri akibat dismenore pada remaja.
Penerapan Buerger Allen Exercise Untuk Meningkatkan Perfusi Ke Ekstremitas Bawah Pada Neuropathy Perifer Penderita Diabetes Melitus Tipe 2
Authors: Alfin Riandi , Rayasari Fitrian , Anggraeni Dewi , Atun Sri
Published: February 2025

Diabetes Melitus (DM) tipe 2 adalah gangguan metabolik yang membutuhkan manajemendan perawatan secara mandiri guna untuk mencegah terjadinya komplikasi mikrovaskulerdan makrovaskuler. Gangguan perfusi pada kaki kerap muncul sebagai tanda gejala awalterjadinya luka pada ekstremitas bawah. Buerger Allen Exercise disebut sebagai sistemlatihan untuk insufisiensi aliran darah arteri pada ekstremitas bawah dengan mengandalkanmuscle pump dan gaya gravitasi untuk meningkatkan perfusi perifer. Tujuan penelitianini adalah mengetahui pengaruh Buerger Allen Exercise terhadap peningkatan perfusiekstremitas bawah pada neuropathy perifer diabetes melitus tipe 2. Metode penelitianyang dilakukan yakni pre eksperimen dengan dengan one group pretest dan posttest.Teknik pemilihan sampel yang digunakan yakni purposive sampling dengan jumlah 15responden. Uji statistik menggunakan uji Wilcoxon dan Paired T Test. Instrumen yangdigunakan yakni lembar observasi, sphygmomanometer dan doppler vaskuler. Hasil ujiunivariat didapatkan nilai rata-rata ABI sebelum intervensi pada kaki kiri 0,96 dan kakikanan 1,00, sementara itu setelah diberikan intervensi didapatkan pada kaki kiri 0,96dan kaki kanan 1,11. Hasil uji bivariat didapatkan terdapat nilai ABI kaki kiri adalahp=0,000< 0,05 dan Kaki kanan adalah p=0,003 < 0,05, hal ini menunjukan adanyaperbedaan nilai ABI pada ekstremitas bawah sebelum dan sesudah intervensi (p-value<0.05). Pelaksanaan Buerger Allen Exercise dapat dilakukan di rumah sakit denganinterval waktu yang sudah dijadwalkan oleh perawat sebagai pencegahan komplikasiakibat penurunan perfusi pada ekstremitas bawah.
Hubungan Status Gizi Dengan Menarche Pada Siswi Sekolah Dasar Dan Madrasah Ibtidaiyah
Authors: Pipih Pipih Napisah , Agustin Mesya Dwi , Herawati Yanti
Published: February 2025

Menarche merupakan kondisi seorang remaja putri yang mengalami menstruasi pertama.Saat ini di Indonesia yang mengalami percepatan menarche sebesar 0,22% per tahundengan rentang usia ≤11 tahun. Tujuan penelitian, yaitu mengetahui hubungan statusgizi dengan menarche pada siswi SDN dan MI. Jenis penelitian, yaitu korelasional.Populasi, yaitu semua siswi kelas IV dan V SD Negri dan MI. Jumlah sampel sebanyak142 siswi. Teknik sampling menggunakan total sampling. Instrument menggunakanlembar observasi, timbangan berat badan, dan meteran tinggi badan. Analisis bivariatmenggunakan uji rank spearman. Hasil penelitian menunjukan bahwa karakteristikresponden, yaitu kelas 5 (52,1%), umur ≤11 tahun (83,8%), belum menarche (74,6%),dan status gizi baik (65,5%). Hasil uji rank spearman menunjukan bahwa bahwa terdapathubungan antara status gizi dan menarche secara signifikan (p-value 0,000). Terbuktidengan dari sebanyak 142 siswi, sebagian besar responden mengalami status gizi baikdan belum menarche (59,1%). Sementara itu, hasil uji status gizi dan usia menarchemenunjukan bahwa terdapat hubungan secara signifikan (p-value 0,002). Terbukti dengandari sebanyak 36 siswi yang sudah menarche, terdapat status gizi berlebih dan mengalamimenarche ≤11 tahun (33,3%) dan obesitas (41,7%). Kesimpulannya, terdapat hubunganyang signifikan antara status gizi dengan menarche pada siswi SD Negeri dan MI. Olehkarena itu, pihak sekolah diharapkan dapat memberikan informasi dan pemahaman terkaitpentingnya gizi seimbang, serta melibatkan orang tua dalam pengaturan pola makan siswi,khususnya saat pembagian rapor.
STUDI WAKTU DAN OPTIMISASI DALAM PENGOLAHAN MINUMAN KOPI DENGAN KERANGKA KERJA MOST
Authors: Sabilah Ade Irpan
Published: February 2025

Pengolahan minuman kopi merupakan proses penting dalam industri kopi yang tengah berkembang pesat. Mulai dari pemilihan biji kopi hingga penyajian kepada konsumen, setiap tahapan pengolahan memegang peranan penting dalam menentukan kualitas akhir minuman kopi. Penelitian ini memberikan pemahaman yang mendalam tentang aspek waktu dalam proses produksi dan menjadi landasan untuk meningkatkan efisiensi proses produksi secara keseluruhan melalui analisis kerangka kerja MOST (Maynard Operation Sequence Technique). Metode penelitian yang dilakukan adalah dengan mengumpulkan data tentang proses produksi kopi mulai dari pemilihan biji kopi hingga penyajian kepada konsumen. Dari sisi pembeli, waktu idle mencapai 260 detik, sedangkan waktu kerja hanya 105 detik, sehingga total waktu yang dibutuhkan adalah 365 detik. Sementara itu, dari sisi operator, waktu idle tercatat selama 60 detik, dengan waktu kerja mencapai 80 detik, sehingga total waktu yang dibutuhkan adalah 100 detik. Dari sisi mesin, waktu idle tercatat selama 60 detik, dengan waktu kerja mencapai 220 detik, sehingga total waktu yang dibutuhkan adalah 280 detik. Temuan penelitian menunjukkan bahwa dengan menerapkan kerangka kerja MOST, waktu produksi minuman kopi dapat dikurangi secara signifikan dengan melakukan penyesuaian pada beberapa tahap produksi, seperti penggilingan biji kopi, proses pembuatan kopi, dan penyajian kepada pelanggan. Temuan ini memberikan wawasan berharga bagi industri kopi untuk meningkatkan efisiensi proses produksi dan mengoptimalkan pengalaman konsumen.
MENINGKATKAN PRODUKTIVITAS PRODUKSI PT.TAHU SUMEDANG DENGAN METODE APC
Authors: Zaharuddin Zaharuddin , Fauzi Muhammad , Utama Denny Wallady
Published: February 2025

Pesatnya perkembangan industri dan semakin ketatnya persaingan antar perusahaan mendorong perusahaan untuk mempertahankan eksistensinya dengan merencanakan dan mengembangkan strategi bersaing. Peningkatan efisiensi menjadi kunci untuk mencapai manfaat yang lebih besar, seperti peningkatan efektivitas aset dalam pengembangan produk dan pencapaian target produksi. Mengukur produktivitas merupakan indikator penting dalam pengelolaan sumber daya perusahaan. Pabrik Tahu ABC Sumedang sebagai industri rumah tangga mengalami penurunan produktivitas akibat penggunaan sumber daya yang kurang optimal, seperti tenaga kerja, bahan baku, energi dan modal. Metode American Productivity Center (APC) digunakan untuk mengukur produktivitas dan mengidentifikasi faktor-faktor penyebab penurunan produktivitas. Hasil pengukuran menunjukkan adanya penurunan produktivitas pada periode 2 dan 3 dibandingkan periode dasar. Berdasarkan analisis indeks produktivitas, indeks profitabilitas, dan indeks perbaikan harga, perbaikan yang diperlukan antara lain penghematan dan pemantauan penggunaan bahan baku dan energi, serta pengurangan modal yang kurang optimal. Perusahaan dapat menerapkan solusi perbaikan untuk meningkatkan produktivitas produksi dan memperkuat daya saing di pasar.
STRATEGI PENGEMBANGAN PRODUK UMKM RAMEN DI KOTA BATAM: PENDEKATAN HOUSE OF QUALITY UNTUK KEUNGGULAN KOMPETITIF
Authors: Putri Angela , Lawi Ansarullah , Bora M. Ansyar
Published: February 2025

Penelitian ini bertujuan untuk menganalisis dan memprioritaskan kebutuhan pelanggan menggunakan pendekatan House of Quality (HoQ) pada UMKM ramen di Kota Batam, serta menghubungkan kebutuhan pelanggan dengan fitur teknis yang relevan. Data dikumpulkan melalui survei terhadap 38 konsumen UMKM “Uru Ramen” di Batam, diikuti dengan analisis validitas dan reliabilitas instrumen survei menggunakan metode statistik. Hasil menunjukkan bahwa semua item survei valid dan reliabel. Analisis HoQ mengungkapkan bahwa kualitas bahan baku dan kebersihan merupakan prioritas utama bagi pelanggan. Pemilihan dan pengawasan pemasok serta jadwal pembersihan rutin menjadi faktor teknis yang paling berpengaruh dalam memenuhi kebutuhan tersebut. Studi ini juga menunjukkan bahwa UMKM “Uru Ramen” memiliki kekuatan dalam kualitas rasa dengan rata-rata nilai pengguna sebesar 41.289, namun kelemahan dalam porsi harga dan kemudahan akses. Rekomendasi diberikan untuk meningkatkan aspek-aspek yang kurang kompetitif dan mempertahankan kualitas bahan baku serta kebersihan guna meningkatkan kepuasan pelanggan dan daya saing UMKM ramen di Kota Batam.
Education and Training Assessment and Artificial Intelligence. A Pragmatic Guide for Educators
Authors: Newton Philip M. , Jones Sue
Published: February 2025

The emergence of ChatGPT and similar new Generative AI tools has created concern about the validity of many current assessment methods in higher education, since learners might use these tools to complete those assessments. Here we review the current evidence on this issue and show that for assessments like essays and multiple-choice exams, these concerns are legitimate: ChatGPT can complete them to a very high standard, quickly and cheaply. We consider how to assess learning in alternative ways, and the importance of retaining assessments of foundational core knowledge. This evidence is considered from the perspective of current professional regulations covering the professional registration of Biomedical Scientists and their Health and Care Professions Council (HCPC) approved education providers, although it should be broadly relevant across higher education.
Farmers’ adaptation strategies to climate change on agricultural production in Arsi zone, Oromia National Regional State of Ethiopia
Authors: Dawid Ibsa , Boka Ephrem
Published: February 2025

Climate change has a greater influence on agricultural production due to the effects of floods, droughts, and crop pests. The objective of the study was to explore farmers’ perceptions of climate change, adaptation strategies, constraints to adaptation strategies, and identify factors affecting the choice of climate change that affect adaptation strategies for agricultural production. Data was collected from 303 households selected using a multistage sampling technique to collect data using structured questionnaires. Descriptive statistics, Mann–Kendall statistical test, the weighted average index, and a multivariate probit model were used. The result shows that farmers used multiple adaptation strategies, mostly improved varieties, improved livestock, and mixed farming. Farmers’ choices of adaptation strategies were affected by education, household size, cooperative membership, extension services, climate information, perception of climate change, and farm income. Thus, efforts to create awareness about climate change through mass media, extension services, and enabling cooperative unions are essential.
Cluster analysis of digital competencies among professors in higher education
Authors: Chahuán-Jiménez Karime , Lara-Yergues Eduardo , Garrido-Araya Dominique , Salum-Alvarado Elena , Hurtado-Arenas Paulina , Rubilar-Torrealba Rolando
Published: February 2025

Purpose

This research focuses on the diagnosis and clustering of professor higher education in relation to digital competencies, based on different levels of digital competency development.

Methods

The methodology employed in this study involved an Ordinary Least Squares (OLS) regression analysis and cluster analysis using K-means clustering algorithm, considering the Silhouette score, based on the responses obtained through a questionnaire DigComEdu framework, and adjusted according to the experts who conducted a validity analysis.

Results

The findings indicate that, for the sample professor who answers the questionnaire voluntarily and confidentially, considering margin of error of 5%, a confidence level of 95%, and a response distribution of 50%, corresponding to 314 professors, with a Crombach’s alpha of 0.56, there is no relation between the variables of investigation, age, gender, academic degree, academic hierarchy, and years in academy and the level of digital competencies among professors. Regarding the clustering analysis, specifically using the K-means clustering algorithm, four distinct clusters are identified based on the questionnaire scores, aligning with findings from Silhouette score and Quadratic error by number of clusters.

Discussion

This research reveals that professors in higher education span all four levels of competency as defined by the DigComEdu model, primarily falling within the intermediate levels of digital competencies. Clustering analysis further provides insights for the implementation of enhancement and development policies, with the aim of guiding professors toward more complex digital activities, ultimately achieving the highest level of digital competencies. This, in turn, fosters improved teaching practices and, consequently, enhances the teaching experience.
A novel LC-MS/MS analysis of vitamin D metabolites in mice serum and hair: impact of diet and light exposure
Authors: Hakeem Muhammad K. , Al-Menhali Asma , Elangovan Sampath K. , Shah Iltaf
Published: February 2025

Introduction

Numerous physiological systems, such as the functioning of the immune system, bone health, and the regulation of expression of genes, depend critically on vitamin D. Considering the significance of vitamin D for health, it is critical to understand how it is metabolized and the factors that affect its levels.

Methods

The objective of this study was to develop and validate an LC-MS/MS method to examine the effects of light exposure and dietary vitamin D consumption on the levels of vitamin D and its metabolites in a mouse model under consistent growth conditions throughout the year. Serum and hair samples from mice were analyzed under various experimental conditions for vitamin D and its metabolites using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The experimental conditions included a vitamin D-deficient diet, a vitamin D-standard diet, and changes in ambient light exposure ranging from complete darkness to a regular light-dark cycle.

Results

Mice fed a standard vitamin D diet and exposed to a regular light-dark cycle exhibited significantly higher levels of 25OHD3 in both serum and hair, indicating the synergistic effect of dietary vitamin D intake and light exposure. Mice fed a standard vitamin D diet but kept in continuous darkness showed moderately elevated 25OHD3 levels, demonstrating the efficacy of dietary vitamin D in maintaining adequate levels despite the absence of light. Conversely, mice fed a vitamin D-deficient diet and housed in darkness displayed 25OHD3 levels below the limit of quantification, highlighting the combined detrimental effects of dietary deficiency and lack of light exposure.

Discussion

This study provides valuable insights into the complex interplay between dietary vitamin D intake, light exposure, and the regulation of vitamin D metabolism in mice. Moreover, our results underscore the potential implications for human health, suggesting the importance of adequate vitamin D intake and sunlight exposure in maintaining optimal vitamin D levels. Further research in this area has the potential to unveil additional factors influencing vitamin D metabolism, offering valuable insights into strategies for optimizing vitamin D levels in both animal models and human subjects.
Mechanisms of oxidative stress-induced sperm dysfunction
Authors: Wang Yutao , Fu Xun , Li Hongjun
Published: February 2025

Oxidative stress plays a pivotal role in male infertility by impairing sperm function through various molecular mechanisms. This review explores the impact of excessive reactive oxygen species (ROS) on spermatozoa, particularly focusing on lipid peroxidation, DNA fragmentation, and protein oxidation. Lipid peroxidation damages sperm membranes, reducing fluidity and motility. ROS-induced DNA fragmentation compromises genetic integrity, potentially leading to infertility and adverse offspring outcomes. Protein oxidation alters key structural proteins, impairing sperm motility and the ability to fertilize an egg. The primary sources of oxidative stress in sperm include leukocyte activity, mitochondrial dysfunction, and environmental factors such as smoking and pollution. Despite the presence of natural antioxidant defenses, spermatozoa are particularly vulnerable due to limited repair mechanisms. The review highlights the importance of early intervention through antioxidant therapies and lifestyle changes to mitigate the detrimental effects of oxidative stress on male fertility. Further research is essential to enhance therapeutic approaches and improve reproductive outcomes.
Single-cell sequencing elucidates the mechanism of NUSAP1 in glioma and its diagnostic and prognostic significance
Authors: Zhao Meng-Yu , Shen Zhao-Lei , Dai Hongzhen , Xu Wan-Yan , Wang Li-Na , Gu Yu- , Zhao Jie-Hui , Yu Tian-Hang , Wang Cun-Zhi , Xu Jia-feng , Chen Guan-Jun , Chen Dong-Hui , Hong Wen-Ming , Zhang Fang
Published: February 2025

Background

Personalized precision medicine (PPPM) in cancer immunology and oncology is a rapidly advancing field with significant potential. Gliomas, known for their poor prognosis, rank among the most lethal brain tumors. Despite advancements, there remains a critical need for precise, individualized treatment strategies.

Methods

We conducted a comprehensive analysis of RNA-seq and microarray data from the TCGA and GEO databases, supplemented by single-cell RNA sequencing (scRNA-seq) data from glioma patients. By integrating single-cell sequencing analysis with foundational experiments, we investigated the molecular variations and cellular interactions within neural glioma cell subpopulations during tumor progression.

Results

Our single-cell sequencing analysis revealed distinct gene expression patterns across glioma cell subpopulations. Notably, differentiation trajectory analysis identified NUSAP1 as a key marker for the terminal subpopulation. We found that elevated NUSAP1 expression correlated with poor prognosis, prompting further investigation of its functional role through both cellular and animal studies.

Conclusions

NUSAP1-based risk models hold potential as predictive and therapeutic tools for personalized glioma treatment. In-depth exploration of NUSAP1’s mechanisms in glioblastoma could enhance our understanding of its response to immunotherapy, suggesting that targeting NUSAP1 may offer therapeutic benefits for glioma patients.
Bronchus-associated lymphoid tissue in lung transplantation: a facilitator of rejection or regulator of tolerance?
Authors: Wein Alexander N. , Liu Charles R. , Kreisel Daniel
Published: February 2025

The role of bronchus-associated lymphoid tissue (BALT) in the regulation of immune responses to transplanted lungs remains an area of interest and controversy. Early studies in a rat pulmonary transplant model suggested BALT may accelerate rejection of grafts by inducing a local and systemic inflammatory response. Such observations were corroborated in intrapulmonary tracheal transplant models in the rat. While some human studies have described the presence of BALT in grafts that have been chronically rejected, others did not observe an association between induction of BALT and adverse outcomes. More recent investigations have found that BALT, enriched in immunoregulatory cell populations, is induced in tolerant mouse lung allografts, suggesting that such structures may be protective against rejection. Thus, the role of BALT in lung transplantation biology is complex. Insights gained from studies that focus on the role of BALT in lung transplantation may be harnessed to develop new therapies.
Sharks in Macaronesia and Cabo Verde: species richness, conservation status and anthropogenic pressures
Authors: Varela Jaquelino , Santos Catarina Pereira , Nunes Emanuel , Pissarra Vasco , Pires Stiven , Ribeiro Bárbara P. , Vieira Eduarda , Repolho Tiago , Queiroz Nuno , Freitas Rui , Rosa Rui
Published: February 2025

The northeast Atlantic Ocean contains multiple habitats considered critical for shark conservation, including nursery areas, migratory corridors and aggregation sites. In this context, updating knowledge on shark diversity and the threats affecting them in this region is essential to defining priorities and implementing the right management and conservation measures. Here, we show that Macaronesian and Cabo Verde marine ecoregions are home to 78 shark species (comprising 26 families), and 56% are threatened with extinction. The Canary Islands revealed the greatest richness (with 56 species), followed by Cabo Verde (53), Madeira (52), and the Azores (45). Cabo Verde presents fewer similarities with the rest of the islands. We also found that: i) Azores share more species with the Canary Islands than Madeira (despite the greater geographical proximity with the latter), and ii) there are no oviparous species in the Cabo Verde archipelago, contrary to the Canary Islands (5), the Azores (4), and Madeira (3). Fishing and habitat degradation are the most relevant anthropogenic pressures for the region, with Cabo Verde having the highest number of endangered species (66%) and a greater magnitude and diversity of threats. As such, this archipelago presents the highest priority area for shark conservation due to the intense industrial fishing in its waters, poor management measures in combination with its greater vulnerability to climate change.
Risk assessment to Barents Sea ecosystem services: an expert-based approach
Authors: Pham Thuy Thi Thanh , Armstrong Claire W. , Solvang Hiroko Kato , Skern-Mauritzen Mette
Published: February 2025

This study aims to investigate the risks posed by climate change and anthropogenic activities on ecosystem services in the Barents Sea, Norway. Using an expert assessment approach, we identify which ecosystem services are at high risk and the human activities and pressures contributing to these risks. The findings indicate that risks vary across ecosystem services, activities, and pressures; however, most are categorized as medium or low. Biodiversity, as a cultural service, and fish/shellfish, as a provisioning service, are identified as the two most threatened ecosystem services. In contrast, educational services are perceived as the least impacted. Temperature change is found to have the greatest impact on the services. Experts are generally uncertain about the risk levels; however, fish/shellfish and biodiversity are the two services associated with the least uncertainty. The results highlight the limited knowledge regarding risks to ecosystem services in the Barents Sea. The study emphasizes the need for future research to address these knowledge gaps and discusses where management efforts should be focused.
Comprehensive analysis of ionomic profiling in Chlorella exposed to chlorpyrifos
Authors: Yu Qing-Qing , Zhang Yulong , Zhao Shiyuan , Pang Min , Jiang Pei , Qu Pei
Published: February 2025

Introduction

Chlorpyrifos (CPF), a widely used organophosphorus insecticide, is highly toxic to non-target aquatic organisms and has relatively high persistence in water, posing a serious threat to marine ecosystems. However, little is known about the toxicological mechanism of CPF on marine microalgae, which is the main primary producer in the marine ecosystem.

Methods

This study explored the ion changes of microalgae Chlorella vulgaris under the stress of CPF through Inductively Coupled Plasma Mass Spectrometry (ICP-MS).

Results

Significant disparities in ionomics among control and treatment group were observed through pattern recognition analysis (principal component analysis, PCA; orthogonal partial least squares discriminant analysis, OPLS-DA), indicating that CPF may impede their growth by disrupting the homeostasis of crucial elements within algal cells.

Discussion

This study elucidated the inhibitory impact of CPF on green algae growth and its potential mechanism of toxicity through ICP-MS, providing crucial insights for a comprehensive understanding of the influence of organophosphorus pesticides on aquatic ecosystems.
The endocannabinoid system and ophthalmic pathologies: a review of molecular mechanisms and its implications for clinical practice
Authors: Charytoniuk Tomasz , Półjanowski Stanisław , Michalak Mateusz , Kaźmierczak Karolina , Kałużny Bartłomiej
Published: February 2025

Within the last decade the role of the endocannabinoid system (ECS) has been a significant part of ophthalmic research, including both ocular physiology and the development of eye pathologies. It is known that this widespread cell-signaling system is involved in retinal neurobiological processes, including visual signal processing, as well as neurotransmission. Furthermore, various research indicated the involvement of ECS in the molecular basis of various pathologies, mostly glaucoma, diabetic retinopathy, and age-related macular degeneration (AMD). Therefore, the researchers believe that this biological system, its receptors, pathways, and ligands might be considered as an auxiliary compound to reduce the number of patients suffering from ophthalmic diseases. Despite presented in the literature effects of the endocannabinoid system in the eye, none of the current ECS reviews presented a comprehensive description of the endocannabinoid system, its compounds, and, subsequently ophthalmic disorders. Thus, the aim of this review was to summarize all the major data, including the most up-to-date research, concerning a correlation between the endocannabinoid system and the major ophthalmic pathologies.
In vivo confocal microscopic study of cornea verticillata and limbus deposits in patients with Fabry disease
Authors: Zhou Xuecong , Zhao Yawen , Li Yingsi , Yuan Yujing , Yan Xiaoming , Zhang Wei , Wu Yuan
Published: February 2025

Purpose

This study was aimed to investigate the microstructure characteristics of cornea verticillata and limbus deposits in patients with Fabry disease (FD) using in vivo confocal microscopy (IVCM).

Methods

A total of 60 eyes from 30 patients diagnosed with FD were examined and compared with 36 eyes from 18 healthy controls in this prospective, cross-sectional, controlled, single-center study. The initial assessment of cornea verticillata (CV) was conducted using slit-lamp microscopy. Subsequently, IVCM was performed to assess deposits in the corneal and limbal epithelium. We compared the differences between the sexes (heterozygous and hemizygous) and phenotypes (classical and non-classical).

Results

The epithelial deposit detection rate with IVCM was statistically higher (52/60, 86.67%) compared to the biomicroscopic evaluation of CV using a silt lamp (46/60, 76.67%) (p = 0.031). A higher prevalence of corneal epithelial deposits was observed in the classical phenotype as compared to the non-classical phenotype (p = 0.023). Surprisingly, cardiac variants previously lacking cornea verticillata show a high prevalence (85.71%) of corneal epithelial deposits under IVCM. The prevalence and severity of deposits, especially in limbal epithelial rete pegs, were higher in FD than in controls (p < 0.001).

Conclusion

Compared with slit-lamp microscopy, IVCM provides a more effective tool for examining the epithelial deposits in patients with FD. Patients with FD demonstrated a profound bilateral increase in corneal epithelial deposits and limbal hyperreflective cells compared to controls, with more prominent pathological changes observed in classical phenotype individuals. The high prevalence of epithelial deposits observed through IVCM in the cardiac variant highlights the essential ability of IVCM as an effective diagnostic tool.
Identification of early prediction biomarkers of severity in patients with severe fever with thrombocytopenia syndrome based on plasma proteomics
Authors: Zhang Qian , Jiang Zhengyi , Jiang Nan , Shi Luchen , Zhao Jiaying , Zhao Jie , Ouyang Ke , Huang Huaying , Zhang Yaqin , Dai Yan , Hu Nannan , Shi Ping , Han Yaping , Jin Ke , Li Jun
Published: February 2025

Background

Severe fever with thrombocytopenia syndrome (SFTS) is a newly emerging infectious disease. Given its rapid disease progression and high mortality rate, early warning is crucial in improving the outcomes, However, to date, relevant comprehensive predictors or an effective prediction model are still poorly explored.

Methods

A plasma proteomic profile was performed at early stages in patients with SFTS. Functional clustering analysis was used to select the candidate proteins and then validate their expression by ELISA. A cohort consisting of 190 patients with SFTS was used to develop the predictive model for severe illness and subsequently validate it in a new cohort consisting of 93 patients with SFTS.

Results

A significant increase in plasma proteins associated with various functional clusters, such as the proteasomal protein catabolic process, phagocytosis, and humoral immune response, was observed in severe SFTS patients. High levels of four proteins including NID1, HSP90α, PSMA1, and VCAM1 were strongly correlated with multi-organ damage and disease progression. A prediction model was developed at the early stage to accurately predict severe conditions with the area under the curve of 0.931 (95% CI, 0.885, 0.963).

Conclusion

The proteomic signatures identified in this study provide insights into the potential pathogenesis of SFTS. The predictive models have substantial clinical implications for the early identification of SFTS patients who may progress to severe conditions.
Comprehensive analysis of lncRNAs and mRNAs revealed potential participants in the process of avian reovirus infection
Authors: Zhang Shaqiu , Li Jinkang , Wang Mingshu , Jia Renyong , Chen Shun , Liu Mafeng , Zhu Dekang , Zhao Xinxin , Wu Ying , Yang Qiao , Huang Juan , Ou Xumin , Sun Di , Tian Bin , He Yu , Wu Zhen , Cheng Anchun
Published: February 2025

Avian reovirus (ARV), a double-stranded RNA virus, frequently induces immunosuppression in poultry, leading to symptoms such as irregular bleeding and spleen necrosis in infected ducks. Since 2017, the morbidity and mortality rates associated with ARV infection in poultry have been on the rise, progressively emerging as a significant viral disease impacting the duck farming industry in China. In our study, we collected duck embryo fibroblasts 18 h post-infection with ARV and conducted transcriptome sequencing analysis. The analysis revealed that 3,818 mRNA expressions were up-regulated, 4,573 mRNA expressions were down-regulated, 472 long noncoding RNAs (LncRNAs) were up-regulated, and 345 lncRNAs were down-regulated. We employed qRT-PCR to validate the sequencing results, confirming their accuracy. The transcriptome data indicated significant upregulation of the PARP9, TLR7, TRIM33, and ATG5 genes, suggesting their potential involvement in ARV infection. Notably, our study identified a novel functional lncRNA, MSTRG.9284.1 (It was named linc000889 in the present study), which inhibits the replication of ARV at the transcriptional, translational levels and viral titer. Overall, this study has identified numerous ARV-induced differentially expressed mRNAs and lncRNAs, including the functional lncRNA linc000889 that inhibits ARV replication. This discovery provides new insights into the mechanisms of ARV infection and may contribute to the development of new prevention and treatment strategies.
Universal slip detection of robotic hand with tactile sensing
Authors: Zhao Chuangri , Yu Yang , Ye Zeqi , Tian Ziyang , Zhang Yifan , Zeng Ling-Li
Published: February 2025

Slip detection is to recognize whether an object remains stable during grasping, which can significantly enhance manipulation dexterity. In this study, we explore slip detection for five-finger robotic hands being capable of performing various grasp types, and detect slippage across all five fingers as a whole rather than concentrating on individual fingertips. First, we constructed a dataset collected during the grasping of common objects from daily life across six grasp types, comprising more than 200 k data points. Second, according to the principle of deep double descent, we designed a lightweight universal slip detection convolutional network for different grasp types (USDConvNet-DG) to classify grasp states (no-touch, slipping, and stable grasp). By combining frequency with time domain features, the network achieves a computation time of only 1.26 ms and an average accuracy of over 97% on both the validation and test datasets, demonstrating strong generalization capabilities. Furthermore, we validated the proposed USDConvNet-DG in real-time grasp force adjustment in real-world scenarios, showing that it can effectively improve the stability and reliability of robotic manipulation.
Construction of multi-robot platform based on dobot robots
Authors: Han Jinchi , Ma Duojicairang
Published: February 2025

For the researches of cooperative control scheme for multirobot systems, this paper sets up an experimental platform based on dobot robots, which can be used to perform physical experiments to verify related schemes. A distributed scheme is proposed to achieve cooperative control for multirobot systems. Simulation results prove the effectiveness of the distributed scheme. Then, the experimental platform based on dobot robots is built to verify the proposed scheme. Specifically, a computer sends data to the microcontroller inside the host through WiFi communication, then the host distributes data to the slaves. Finally, the physical experiment of related schemes is performed on the experimental platform. Comparing the simulations with the physical experiments, the task is successfully completed on this experimental platform, which proves the effectiveness of the scheme and the feasibility of the platform. The experimental platform developed in this paper possesses the capability to validate various schemes and exhibits strong expandability and practicality.
Secondary endolymphatic hydrops: a clinical and literature overview
Authors: Venkatasamy Aïna , Péporté Anne R. J.
Published: February 2025

Introduction and importance

Secondary endolymphatic hydrops (SEH) is a pathologic condition of the inner ear that usually manifests as episodic vertigo and fluctuating hearing loss, overlapping with other temporal bone pathologies and inner ear diseases.

Methods

We searched Pubmed and the Cochrane database for English-language studies published through July 2024.

Results

Fifty-four relevant studies and reviews were included in this review on secondary endolymphatic hydrops. This review presents a range of the underlying pathologies in endolymphatic hydrops, along with their corresponding radiological findings, while discussing the associated pathophysiological mechanisms. Secondary endolymphatic hydrops may result from cerebellopontine angle tumors, longstanding inner ear conditions, inner ear malformations, intracranial hypotension and recent investigations have highlighted the role of trauma and inflammation as key factors in SEH development.

Discussion

Despite the diverse etiologies of SEH, the findings suggest that many of these conditions share a common final pathway in disrupting endolymphatic fluid balance. This review provides a better understanding of the pathophysiology and etiologies of this intricate disease process, thereby facilitating the diagnosis and treatment of the affected patients.
Predictors and outcomes of angioplasty and stenting in acute intracranial atherosclerosis-related vertebrobasilar artery occlusion
Authors: Park Seol Bin , Baek Byung Hyun , Lee Yun Young , Kim Seul Kee , Park Chan , Lee Byung Chan , Kim Hyoung Ook , Yoon Woong
Published: February 2025

Objective

This study aimed to investigate clinical outcomes and predictors of favorable functional outcomes after endovascular treatment, including emergent angioplasty and stenting, in patients with intracranial atherosclerotic stenosis (ICAS)-related occlusions in vertebrobasilar arteries.

Materials and methods

This retrospective case series included 46 patients with acute occlusions of vertebrobasilar arteries, due to underlying ICAS. All patients underwent a thrombectomy, followed by angioplasty and/or stenting. We performed logistic regression analyses to identify independent predictors of favorable outcomes. A favorable outcome was defined as a score of 0–3 on the 90-day modified Rankin Scale.

Results

Overall, successful reperfusion was achieved in 44/46 (95.7%) patients and 90-day favorable outcomes were achieved in 21/46 patients (45.7%). The 90-day mortality rate was 23.9% (11/46). In a multivariate binary logistic analysis, independent predictors of favorable outcome were hyperlipidemia (OR = 7.866, 95% CI: 1.093–56.590, p = 0.040), admission hyperglycemia (OR = 0.144, 95% CI: 0.023–0.914, p = 0.040), basilar artery occlusion (OR = 0.086, 95% CI: 0.008–0.907, p = 0.041), and treatment with angioplasty alone (OR = 9.779, 95% CI: 1.022–93.525, p = 0.048).

Conclusion

Our findings suggested that emergent angioplasty and/or stenting could yield high rates of successful reperfusion and favorable outcomes in patients with ICAS-related occlusions in vertebrobasilar arteries. Our study also demonstrated that hyperlipidemia and treatment with angioplasty alone were associated with favorable outcomes, in contrast, admission hyperglycemia and basilar artery occlusion were associated with unfavorable outcomes in these patients.
Experimental evidence-based construction of electroacupuncture for ischemic stroke: a meta-analysis and systematic review
Authors: Guo Yaoguang , Hu Sihan , Luo Shiman , Tu Lang , Tang Yao , Zeng Fang
Published: February 2025

Objective

Ischemic stroke represents a leading cause of disability and mortality worldwide, necessitating effective and complementary therapeutic strategies. Electroacupuncture (EA), a modern extension of traditional acupuncture, has garnered attention for its potential neuroprotective effects in ischemic stroke rehabilitation. This meta-analysis and systematic review aim to synthesize current experimental evidence on the efficacy of EA in ischemic stroke models, focusing on neurological outcomes, infarct volumes, and underlying molecular mechanisms.

Methods

A comprehensive search was performed across four databases—Cochrane Library, EMBASE, PubMed, and Web of Science—to identify relevant experimental studies that utilized electroacupuncture (EA) as a therapeutic modality for ischemic stroke in animal models. This search encompassed all literature available from the inception of each library through December 2023. Studies were rigorously screened based on predefined inclusion and exclusion criteria. Data on cerebral infarction volume, neurological deficit scores, cellular apoptosis, and molecular pathways were extracted and analyzed.

Results

Eleven eligible studies involving 302 animals (151 in EA treatment groups and 151 in control groups) were included. Meta-analysis revealed that EA significantly reduced cerebral infarction volumes [MD = −15.78, 95%CI (−21.40, −10.16), p < 0.05] and TUNEL-positive cells [MD = −26.46, 95%CI (−40.40, −12.51), p < 0.05], indicating reduced apoptosis. Improvements were also noted in neurological deficit scores [MD = −0.59, 95%CI (−0.92, −0.27), p < 0.05] and modified Neurological Severity Scores (mNSS) [MD = -5.68, 95%CI (−7.41, −3.95), p < 0.05], highlighting functional recovery. While the analysis showed no significant effect on caspase-3 densities [MD = −0.39, 95%CI (−0.79, 0.02), p > 0.05], a notable increase in Bcl-2 densities suggested an anti-apoptotic mechanism [MD = −0.73, 95%CI (−1.68, 0.21), p > 0.05]. The heterogeneity of the included studies points to complex underlying mechanisms, potentially involving modulation of apoptotic pathways and cerebral blood flow.

Conclusion

This meta-analysis substantiates the neuroprotective potential of EA in ischemic stroke models, primarily through apoptosis modulation and possibly through improved cerebral perfusion. These findings advocate for the integration of EA into stroke rehabilitation protocols and underscore the need for clinical trials to validate its efficacy in human subjects. Our study not only reinforces the therapeutic value of EA but also prompts further investigation into its underlying mechanisms, potentially guiding more effective stroke recovery strategies.