bluuebunny/crossref_metadata_2025 huggingface dataset:
Understanding the Value of Energy Storage for Power System Reliability and Resilience Applications
Authors: Balducci Patrick , Mongird Kendall , Weimar Mark
Published: June 2021

Purpose of Review

The need for energy storage in the electrical grid has grown in recent years in response to a reduced reliance on fossil fuel baseload power, added intermittent renewable investment, and expanded adoption of distributed energy resources. While the methods and models for valuing storage use cases have advanced significantly in recent years, the value of enhanced resilience remains an open research question.

Recent Findings

The findings of the recent research indicate that energy storage provides significant value to the grid, with median benefit values for specific use cases ranging from under $10/kW-year for voltage support to roughly $100/kW-year for capacity and frequency regulation services. While the value of lost load is used widely to estimate the benefits of mitigating short-duration outages, reaching as high as $719/kilowatt-year, there is no consensus when it comes to monetizing the value of improving grid resilience.

This paper presents a use case taxonomy for energy storage and uses the taxonomy to conduct a meta-analysis of an extensive set of energy storage valuation studies. It reviews several approaches for monetizing reliability and resiliency services and presents a proposed approach for valuing resiliency for energy storage investments.
Acute kidney injury (AKI) in patients with Covid-19 infection is associated with ventilatory management with elevated positive end-expiratory pressure (PEEP)
Authors: Ottolina Davide , Zazzeron Luca , Trevisi Letizia , Agarossi Andrea , Colombo Riccardo , Fossali Tommaso , Passeri Mattia , Borghi Beatrice , Ballone Elisabetta , Rech Roberto , Castelli Antonio , Catena Emanuele , Nebuloni Manuela , Gallieni Maurizio
Published: June 2021

Background

Acute kidney injury (AKI) in Covid-19 patients admitted to the intensive care unit (ICU) is common, and its severity may be associated with unfavorable outcomes. Severe Covid-19 fulfills the diagnostic criteria for acute respiratory distress syndrome (ARDS); however, it is unclear whether there is any relationship between ventilatory management and AKI development in Covid-19 ICU patients.

Purpose

To describe the clinical course and outcomes of Covid-19 ICU patients, focusing on ventilatory management and factors associated with AKI development.

Methods

Single-center, retrospective observational study, which assessed AKI incidence in Covid-19 ICU patients divided by positive end expiratory pressure (PEEP) tertiles, with median levels of 9.6 (low), 12.0 (medium), and 14.7 cmH2O (high-PEEP).

Results

Overall mortality was 51.5%. AKI (KDIGO stage 2 or 3) occurred in 38% of 101 patients. Among the AKI patients, 19 (53%) required continuous renal replacement therapy (CRRT). In AKI patients, mortality was significantly higher versus non-AKI (81% vs. 33%, p < 0.0001). The incidence of AKI in low-, medium-, or high-PEEP patients were 16%, 38%, and 59%, respectively (p = 0.002). In a multivariate analysis, high-PEEP patients showed a higher risk of developing AKI than low-PEEP patients (OR = 4.96 [1.1–21.9] 95% CI p < 0.05). ICU mortality rate was higher in high-PEEP patients, compared to medium-PEEP or low-PEEP patients (69% vs. 44% and 42%, respectively; p = 0.057).

Conclusion

The use of high PEEP in Covid-19 ICU patients is associated with a fivefold higher risk of AKI, leading to higher mortality. The cause and effect relationship needs further analysis.

Graphic abstract
Student perceptions of support for civic identity development and identity exploration in a discussion-based U.S. government course
Authors: Denney Sarah M.
Published: June 2021

Undergirded by theories of civic identity development, transformative pedagogies, and the promotion of identity exploration in the academic curriculum, this qualitative study examines five high school students’ perceptions of support for their civic identity development and identity exploration within a twelfth-grade Advanced Placement (AP) U.S. Government course. Classroom observations, teacher and student interviews, and artifact collection over the course of a three-month research period informed individual and cross-case analysis. The findings of this study indicate that the frequent use of discussion-based practices is supportive of identity exploration for students in different statuses of civic identity. All participants perceived support from their AP U.S. Government teacher and all perceived unique value in various class activities. The role of the instructor as an intentional agent for civic identity development and adolescent identity exploration is considered.
Beings of Thought and Action
Authors: Mueller Andy
Published: June 2021

In this book, Andy Mueller examines the ways in which epistemic and practical rationality are intertwined. In the first part, he presents an overview of the contemporary debates about epistemic norms for practical reasoning, and defends the thesis that epistemic rationality can make one practically irrational. Mueller proposes a contextualist account of epistemic norms for practical reasoning and introduces novel epistemic norms pertaining to ends and hope. In the second part Mueller considers current approaches to pragmatic encroachment in epistemology, ultimately arguing in favor of a new principle-based argument for pragmatic encroachment. While the book defends tenets of the knowledge-first programme, one of its main conclusions is thoroughly pragmatist: in an important sense, the practical has primacy over the epistemic.
Chinese Bureaucracy Through Three Lenses: Weberian, Confucian, and Marchian
Authors: Zhou Xueguang
Published: June 2021

Chinese bureaucracy, with its long history and distinctive characteristics, has provided the organizational basis of governance and played a pivotal role in the economic takeoff in recent decades. Chinese bureaucracy also shows intriguing dualism between entrepreneurial activism and bureaucratic inertia, between formal rules and informal institutions, and between high responsiveness and noticeable loose coupling. In this study, I explore these distinctive features of Chinese bureaucracy through three lenses: Weber's comparative-historical approach helps locate Chinese bureaucracy in a distinct mode of domination; the Confucian lens identifies the prevalence of informal institutions that underlie bureaucratic behaviors; and the Marchian lens sheds light on the organized anarchy and set of mechanisms that shape the key characteristics of Chinese bureaucracy.
A 25,000 year record of climate and vegetation change from the southwestern Cape coast, South Africa
Authors: Quick Lynne J. , Chase Brian M. , Carr Andrew S. , Chevalier Manuel , Grobler B. Adriaan , Meadows Michael E.
Published: June 2021

The southwestern Cape of South Africa is a particularly dynamic region in terms of long-term climate change. We analysed fossil pollen from a 25,000 year sediment core taken from a near-coastal wetland at Pearly Beach that revealed that distinct changes in vegetation composition occurred along the southwestern Cape coast. From these changes, considerable variability in temperature and moisture availability are inferred. Consistent with indications from elsewhere in southwestern Africa, variability in Atlantic Meridional Overturning Circulation (AMOC) was identified as a strong determinant of regional climate change. At Pearly Beach, this resulted in phases of relatively drier conditions (~24–22.5 cal ka BP and ~22–18 cal ka BP) demarcated by brief phases of increased humidity from ~24.5–24 cal ka BP and 22.5–22 cal ka BP. During glacial Termination I (~19–11.7 ka), a marked increase in coastal thicket pollen from ~18.5 to 15.0 cal ka BP indicates a substantial increase in moisture availability, coincident, and likely associated with, a slowing AMOC and a buildup of heat in the southern Atlantic. With clear links to glacial and deglacial Earth system dynamics and perturbations, the Pearly Beach record represents an important new contribution to a growing body of data, providing insights into the patterns and mechanisms of southwestern African climate change.
Nutrition within the household: 18th through early 20thcentury female and male statures
Authors: Carson Scott Alan
Published: June 2021

When other measures for material conditions are scarce or unreliable, the use of height is now common to evaluate economic conditions during economic development. However, throughout US economic development, height data by gender have been slow to emerge. Throughout the late 19thand early 20thcenturies, female and male statures remained constant. Agricultural workers had taller statures than workers in other occupations, and the female agricultural height premium was over twice that of males. For both females and males, individuals with fairer complexions were taller than their darker complexioned counterparts. Gender collectively had the greatest explanatory effect associated with stature, followed by age and nativity. Socioeconomic status and birth period had the smallest collective effects with stature.
Omega-3 fatty acid intake during pregnancy and risk of infant maltreatment: a nationwide birth cohort – the Japan Environment and Children's Study
Authors: Matsumura Kenta , Hamazaki Kei , Tsuchida Akiko , Inadera Hidekuni ,   the Japan Environment and Children's Study (JECS) Group
Published: June 2021

Background

Intake of omega-3 polyunsaturated fatty acids (PUFAs) has favorable effects, including reducing violent and aggressive behaviors, but its association with infant maltreatment is unknown. We therefore tested the hypothesis that maternal intake of omega-3 PUFAs is associated with a lower risk of infant maltreatment.

Methods

Participants were 92 191 mothers involved in the ongoing Japan Environment and Children's Study. Omega-3 PUFA intake during pregnancy was measured using a food frequency questionnaire. Infant maltreatment was assessed using a self-reported questionnaire administered at 1 and 6 months postpartum.

Results

Analysis using the lowest quintile of intake as a reference revealed that the adjusted odds ratios (ORs) with 95% confidence intervals (CIs) for cases of ‘hitting’ decreased as quintiles increased, with values for the second to fifth quintiles of 0.93 (95% CI 0.77–1.13), 0.79 (95% CI 0.64–0.97), 0.78 (95% CI 0.64–0.96), and 0.72 (95% CI 0.59–0.89), respectively. Adjusted ORs (95% CIs) for ‘shaking very hard’ at 6 months were 0.87 (0.73–1.04), 0.81 (0.67–0.97), 0.73 (0.61–0.89), and 0.78 (0.65–0.94), respectively. Adjusted ORs for ‘leaving alone at home’ for the second to fifth quintiles were 0.92 (0.87–0.98), 0.91 (0.86–0.97), 0.94 (0.88–0.99), and 0.85 (0.80–0.90), respectively.

Conclusions

Higher maternal intake of omega-3 PUFAs during pregnancy was associated with fewer cases of hitting and violent shaking and leaving the child alone at home, implying a lower risk of infant maltreatment. Our results indicate the potential applicability of omega-3 PUFAs in reducing infant maltreatment.
The influence of prominence cues in 7- to 10-year-olds’ pronoun resolution: Disentangling order of mention, grammatical role, and semantic role
Authors: BLYTHING Liam P. , IRAOLA AZPIROZ Maialen , ALLEN Shanley , HERT Regina , JÄRVIKIVI Juhani
Published: June 2021

In two visual world experiments we disentangled the influence of order of mention (first vs. second mention), grammatical role (subject vs object), and semantic role (proto-agent vs proto-patient) on 7- to 10-year-olds’ real-time interpretation of German pronouns. Children listened to SVO or OVS sentences containing active accusative verbs (küssen “to kiss”) in Experiment 1 (N = 72), or dative object-experiencer verbs (gefallen “to like”) in Experiment 2 (N = 64). This was followed by the personal pronoun er or the demonstrative pronoun der. Interpretive preferences for er were most robust when high prominence cues (first mention, subject, proto-agent) were aligned onto the same entity; and the same applied to der for low prominence cues (second mention, object, proto-patient). These preferences were reduced in conditions where cues were misaligned, and there was evidence that each cue independently influenced performance. Crucially, individual variation in age predicted adult-like weighting preferences for semantic cues (Schumacher, Roberts & Järvikivi, 2017).
Lithostratigraphy, age and distribution of Eocene volcanic sequences on eastern King George Island, South Shetland Islands, Antarctica
Authors: Smellie John L. , Hunt Richard J. , Mcintosh William C. , Esser Richard P.
Published: June 2021

New mapping and dating of volcanic outcrops on the east coast of Admiralty Bay, King George Island, has demonstrated that Eocene volcanic sequences are dominant and also crop out extensively elsewhere, particularly on the eastern part of the island. The sequences can be divided into at least three formations (Hennequin, Cape Vauréal and Carruthers Cliff) together with Eocene strata at Warkocz and near Lions Rump that are currently unassigned stratigraphically. New and recently published40Ar/39Ar ages indicate that all of the formations are Early Eocene in age, mainly Ypresian, extending to Lutetian and possibly even Priabonian time in more easterly outcrops. Compositional contrasts exist between the groups (calc-alkalinevstholeiitic). The formations are mainly composed of lavas, and many show evidence for contemporary inundation by water. They are interbedded with sedimentary rocks deposited mainly during flooding events as debris flows, debris avalanches, hyperconcentrated flows, from traction currents and in lakes. The common presence of juvenile volcanic detritus suggests that the sediments were probably linked to explosive hydrovolcanic eruptions, some of which were possibly rooted in summit ice caps. Other evidence is also permissive, but the presence of Eocene ice on King George Island is not well established at present.
Fast Grain Mapping with Sub-Nanometer Resolution Using 4D-STEM with Grain Classification by Principal Component Analysis and Non-Negative Matrix Factorization
Authors: Allen Frances I. , Pekin Thomas C. , Persaud Arun , Rozeveld Steven J. , Meyers Gregory F. , Ciston Jim , Ophus Colin , Minor Andrew M.
Published: June 2021

High-throughput grain mapping with sub-nanometer spatial resolution is demonstrated using scanning nanobeam electron diffraction (also known as 4D scanning transmission electron microscopy, or 4D-STEM) combined with high-speed direct-electron detection. An electron probe size down to 0.5 nm in diameter is used and the sample investigated is a gold–palladium nanoparticle catalyst. Computational analysis of the 4D-STEM data sets is performed using a disk registration algorithm to identify the diffraction peaks followed by feature learning to map the individual grains. Two unsupervised feature learning techniques are compared: principal component analysis (PCA) and non-negative matrix factorization (NNMF). The characteristics of the PCA versus NNMF output are compared and the potential of the 4D-STEM approach for statistical analysis of grain orientations at high spatial resolution is discussed.
Online teaching: a reflection
Authors: Baddeley Sam
Published: June 2021

This article, written at the start of April 2021, is a personal reflection on what has and hasn't worked in remote/online education. I have drawn on my own experience of teaching over the course of the past year, observations of classroom practice I have undertaken as a mentor and middle leader with responsibility for teaching and learning in my school, and conversations I have had with colleagues in my school and elsewhere; it is, therefore, highly anecdotal, and the reader is asked to bear in mind the fact that, like many others, my journey into online teaching was enforced by the closure of schools during the first nationwide lockdown in March 2020. My core aim during both lockdowns was to provide for my students the best experience possible until such a time as we could all return to the physical classroom. As it became clear towards the end of 2020 and the start of 2021 that we were going to need to return to remote education, I began to think more deeply about the strategies I was employing in my online teaching, how effective they were for my students, and what I might do to maximise their learning experience and outcomes.
Rapid Response to the 2019 Ridgecrest Earthquake With Distributed Acoustic Sensing
Authors: Li Zefeng , Shen Zhichao , Yang Yan , Williams Ethan , Wang Xin , Zhan Zhongwen
Published: June 2021

Rapid seismic deployments after major earthquakes often produce critical data for characterizing postseismic processes. Taking advantage of pre‐existing optical fibers, the recently emerging distributed acoustic sensing (DAS) technology can quickly establish ultra‐dense seismic arrays after the mainshocks. Here we present the first example of such a rapid‐response experiment using four telecommunication fiber optic cables near the 2019 M 7.1 Ridgecrest earthquake in California. By applying template matching to the Ridgecrest DAS array, we detected 6 times more aftershocks than the standard catalog within the three‐month period. The enhanced catalog reveals abundant aftershocks on multiple crosscutting faults near the epicenters of the mainshock and the M 6.4 foreshock. Given the widespread fiber optic networks around the world, DAS has the potential to deliver fast and high‐resolution aftershock monitoring and promote better understanding of earthquake physics.
Multi‐Decadal Carbon Cycle Measurements Indicate Resistance to External Drivers of Change at the Howland Forest AmeriFlux Site
Authors: Hollinger D. Y. , Davidson E. A. , Fraver S. , Hughes H. , Lee J. T. , Richardson A. D. , Savage K. , Sihi D. , Teets A.
Published: June 2021

A long‐standing goal of ecology has been to understand the cycling of carbon in forests. This has taken on new urgency with the need to address a rapidly changing climate. Forests serve as long‐term stores for atmospheric CO2, but their continued ability to take up new carbon is dependent on future changes in climate and other factors such as age. We have been measuring many aspects of carbon cycling at an unmanaged evergreen forest in central Maine, USA, for over 25 years. Here we use these data to address questions about the magnitude and control of carbon fluxes and quantify flows and uncertainties between the different pools. A key issue was to assess whether recent climate change and an aging tree population were reducing annual C storage. Total ecosystem C stocks determined from inventory and quantitative soil pits were about 23,300 g C m−2with 46% in live trees, and 48% in the soil. Annual biomass increment in trees at Howland Forest averaged 161 ± 23 g C m−2 yr−1, not significantly different from annual net ecosystem production (NEP = −NEE) of 211 ± 40 g C m−2 y−1measured by eddy covariance. Unexpectedly, there was a small but significant trend of increasing C uptake through time in the eddy flux data. This was despite the period of record including some of the most climate‐extreme years in the last 125. We find a surprising lack of influence of climate variability on annual carbon storage in this mature forest.
A distribution-centered approach for analyzing human adipocyte size estimates and their association with obesity-related traits and mitochondrial function
Authors: Honecker Julius , Weidlich Dominik , Heisz Simone , Lindgren Cecilia M. , Karampinos Dimitrios C. , Claussnitzer Melina , Hauner Hans
Published: June 2021

Objective

Cell diameter, area, and volume are established quantitative measures of adipocyte size. However, these different adipocyte sizing parameters have not yet been directly compared regarding their distributions. Therefore, the study aimed to investigate how these adipocyte size measures differ in their distribution and assessed their correlation with anthropometry and laboratory chemistry. In addition, we were interested to investigate the relationship between fat cell size and adipocyte mitochondrial respiratory chain capacity.

Methods

Subcutaneous and visceral histology-based adipocyte size estimates from 188 individuals were analyzed by applying a panel of parameters to describe the underlying cell population. Histology-based adipocyte diameter distributions were compared with adipocyte diameter distributions from collagenase digestion. Associations of mean adipocyte size with body mass index (BMI), glucose, HbA1C, blood lipids as well as mature adipocyte mitochondrial respiration were investigated.

Results

All adipocyte area estimates derived from adipose tissue histology were not normally distributed, but rather characterized by positive skewness. The shape of the size distribution depends on the adipocyte sizing parameter and on the method used to determine adipocyte size. Despite different distribution shapes histology-derived adipocyte area, diameter, volume, and surface area consistently showed positive correlations with BMI. Furthermore, associations between adipocyte sizing parameters and glucose, HbA1C, or HDL specifically in the visceral adipose depot were revealed. Increasing subcutaneous adipocyte diameter was negatively correlated with adipocyte mitochondrial respiration.

Conclusions

Despite different underlying size distributions, the correlation with obesity-related traits was consistent across adipocyte sizing parameters. Decreased mitochondrial respiratory capacity with increasing subcutaneous adipocyte diameter could display a novel link between adipocyte hypertrophy and adipose tissue function.
Context-dependent modulation of aggressiveness of pediatric tumors by individual oncogenic RAS isoforms
Authors: Bauer Julia , Cuvelier Nicole , Ragab Nada , Simon-Keller Katja , Nitzki Frauke , Geyer Natalie , Botermann Dominik S. , Elmer Dominik P. , Rosenberger Albert , Rando Thomas A. , Biressi Stefano , Fagin James A. , Saur Dieter , Dullin Christian , Schildhaus Hans-Ulrich , Schulz-Schaeffer Walter , Aberger Fritz , Uhmann Anja , Hahn Heidi
Published: June 2021

A prototypic pediatric cancer that frequently shows activation of RAS signaling is embryonal rhabdomyosarcoma (ERMS). ERMS also show aberrant Hedgehog (HH)/GLI signaling activity and can be driven by germline mutations in this pathway. We show, that in ERMS cell lines derived from sporadic tumors i.e. from tumors not caused by an inherited genetic variant, HH/GLI signaling plays a subordinate role, because oncogenic mutations in HRAS, KRAS, or NRAS (collectively named oncRAS) inhibit the main HH target GLI1 via the MEK/ERK-axis, but simultaneously increase proliferation and tumorigenicity. oncRAS also modulate expression of stem cell markers in an isoform- and context-dependent manner. In Hh-driven murine ERMS that are caused by a Patched mutation, oncHRAS and mainly oncKRAS accelerate tumor development, whereas oncNRAS induces a more differentiated phenotype. These features occur when the oncRAS mutations are induced at the ERMS precursor stage, but not when induced in already established tumors. Moreover, in contrast to what is seen in human cell lines, oncRAS mutations do not alter Hh signaling activity and marginally affect expression of stem cell markers. Together, all three oncRAS mutations seem to be advantageous for ERMS cell lines despite inhibition of HH signaling and isoform-specific modulation of stem cell markers. In contrast, oncRAS mutations do not inhibit Hh-signaling in Hh-driven ERMS. In this model, oncRAS mutations seem to be advantageous for specific ERMS populations that occur within a specific time window during ERMS development. In addition, this window may be different for individual oncRAS isoforms, at least in the mouse.
A preclinical pipeline to evaluate migrastatics as therapeutic agents in metastatic melanoma
Authors: Maiques Oscar , Fanshawe Bruce , Crosas-Molist Eva , Rodriguez-Hernandez Irene , Volpe Alessia , Cantelli Gaia , Boehme Lena , Orgaz Jose L. , Mardakheh Faraz K. , Sanz-Moreno Victoria , Fruhwirth Gilbert O.
Published: June 2021

Background

Metastasis is a hallmark of cancer and responsible for most cancer deaths. Migrastatics were defined as drugs interfering with all modes of cancer cell invasion and thus cancers’ ability to metastasise. First anti-metastatic treatments have recently been approved.

Methods

We used bioinformatic analyses of publicly available melanoma databases. Experimentally, we performed in vitro target validation (including 2.5D cell morphology analysis and mass spectrometric analysis of RhoA binding partners), developed a new traceable spontaneously metastasising murine melanoma model for in vivo validation, and employed histology (haematoxylin/eosin and phospho-myosin II staining) to confirm drug action in harvested tumour tissues.

Results

Unbiased and targeted bioinformatic analyses identified the Rho kinase (ROCK)-myosin II pathway and its various components as potentially relevant targets in melanoma. In vitro validation demonstrated redundancy of several RhoGEFs upstream of RhoA and confirmed ROCK as a druggable target downstream of RhoA. The anti-metastatic effects of two ROCK inhibitors were demonstrated through in vivo melanoma metastasis tracking and inhibitor effects also confirmed ex vivo by digital pathology.

Conclusions

We proposed a migrastatic drug development pipeline. As part of the pipeline, we provide a new traceable spontaneous melanoma metastasis model for in vivo quantification of metastasis and anti-metastatic effects by non-invasive imaging.
Divide-and-conquer: machine-learning integrates mammalian and viral traits with network features to predict virus-mammal associations
Authors: Wardeh Maya , Blagrove Marcus S. C. , Sharkey Kieran J. , Baylis Matthew
Published: June 2021

Our knowledge of viral host ranges remains limited. Completing this picture by identifying unknown hosts of known viruses is an important research aim that can help identify and mitigate zoonotic and animal-disease risks, such as spill-over from animal reservoirs into human populations. To address this knowledge-gap we apply a divide-and-conquer approach which separates viral, mammalian and network features into three unique perspectives, each predicting associations independently to enhance predictive power. Our approach predicts over 20,000 unknown associations between known viruses and susceptible mammalian species, suggesting that current knowledge underestimates the number of associations in wild and semi-domesticated mammals by a factor of 4.3, and the average potential mammalian host-range of viruses by a factor of 3.2. In particular, our results highlight a significant knowledge gap in the wild reservoirs of important zoonotic and domesticated mammals’ viruses: specifically, lyssaviruses, bornaviruses and rotaviruses.
Impact of an accelerated melting of Greenland on malaria distribution over Africa
Authors: Chemison Alizée , Ramstein Gilles , Tompkins Adrian M. , Defrance Dimitri , Camus Guigone , Charra Margaux , Caminade Cyril
Published: June 2021

Studies about the impact of future climate change on diseases have mostly focused on standard Representative Concentration Pathway climate change scenarios. These scenarios do not account for the non-linear dynamics of the climate system. A rapid ice-sheet melting could occur, impacting climate and consequently societies. Here, we investigate the additional impact of a rapid ice-sheet melting of Greenland on climate and malaria transmission in Africa using several malaria models driven by Institute Pierre Simon Laplace climate simulations. Results reveal that our melting scenario could moderate the simulated increase in malaria risk over East Africa, due to cooling and drying effects, cause a largest decrease in malaria transmission risk over West Africa and drive malaria emergence in southern Africa associated with a significant southward shift of the African rain-belt. We argue that the effect of such ice-sheet melting should be investigated further in future public health and agriculture climate change risk assessments.
Barrel cortex plasticity after photothrombotic stroke involves potentiating responses of pre-existing circuits but not functional remapping to new circuits
Authors: Zeiger William A. , Marosi Máté , Saggi Satvir , Noble Natalie , Samad Isa , Portera-Cailliau Carlos
Published: June 2021

Recovery after stroke is thought to be mediated by adaptive circuit plasticity, whereby surviving neurons assume the roles of those that died. However, definitive longitudinal evidence of neurons changing their response selectivity after stroke is lacking. We sought to directly test whether such functional “remapping” occurs within mouse primary somatosensory cortex after a stroke that destroys the C1 barrel. Using in vivo calcium imaging to longitudinally record sensory-evoked activity under light anesthesia, we did not find any increase in the number of C1 whisker-responsive neurons in the adjacent, spared D3 barrel after stroke. To promote plasticity after stroke, we also plucked all whiskers except C1 (forced use therapy). This led to an increase in the reliability of sensory-evoked responses in C1 whisker-responsive neurons but did not increase the number of C1 whisker-responsive neurons in spared surround barrels over baseline levels. Our results argue against remapping of functionality after barrel cortex stroke, but support a circuit-based mechanism for how rehabilitation may improve recovery.
Scalable, highly stable Si-based metal-insulator-semiconductor photoanodes for water oxidation fabricated using thin-film reactions and electrodeposition
Authors: Lee Soonil , Ji Li , De Palma Alex C. , Yu Edward T.
Published: June 2021

Metal-insulator-semiconductor (MIS) structures are widely used in Si-based solar water-splitting photoelectrodes to protect the Si layer from corrosion. Typically, there is a tradeoff between efficiency and stability when optimizing insulator thickness. Moreover, lithographic patterning is often required for fabricating MIS photoelectrodes. In this study, we demonstrate improved Si-based MIS photoanodes with thick insulating layers fabricated using thin-film reactions to create localized conduction paths through the insulator and electrodeposition to form metal catalyst islands. These fabrication approaches are low-cost and highly scalable, and yield MIS photoanodes with low onset potential, high saturation current density, and excellent stability. By combining this approach with a p+n-Si buried junction, further improved oxygen evolution reaction (OER) performance is achieved with an onset potential of 0.7 V versus reversible hydrogen electrode (RHE) and saturation current density of 32 mA/cm2 under simulated AM1.5G illumination. Moreover, in stability testing in 1 M KOH aqueous solution, a constant photocurrent density of ~22 mA/cm2 is maintained at 1.3 V versus RHE for 7 days.
LEAFY COTYLEDON1 expression in the endosperm enables embryo maturation in Arabidopsis
Authors: Song Jingpu , Xie Xin , Chen Chen , Shu Jie , Thapa Raj K. , Nguyen Vi , Bian Shaomin , Kohalmi Susanne E. , Marsolais Frédéric , Zou Jitao , Cui Yuhai
Published: June 2021

The endosperm provides nutrients and growth regulators to the embryo during seed development. LEAFY COTYLEDON1 (LEC1) has long been known to be essential for embryo maturation. LEC1 is expressed in both the embryo and the endosperm; however, the functional relevance of the endosperm-expressed LEC1 for seed development is unclear. Here, we provide genetic and transgenic evidence demonstrating that endosperm-expressed LEC1 is necessary and sufficient for embryo maturation. We show that endosperm-synthesized LEC1 is capable of orchestrating full seed maturation in the absence of embryo-expressed LEC1. Inversely, without LEC1 expression in the endosperm, embryo development arrests even in the presence of functional LEC1 alleles in the embryo. We further reveal that LEC1 expression in the endosperm begins at the zygote stage and the LEC1 protein is then trafficked to the embryo to activate processes of seed maturation. Our findings thus establish a key role for endosperm in regulating embryo development.
Catalytic asymmetric nucleophilic fluorination using BF3·Et2O as fluorine source and activating reagent
Authors: Zhu Weiwei , Zhen Xiang , Wu Jingyuan , Cheng Yaping , An Junkai , Ma Xingyu , Liu Jikun , Qin Yuji , Zhu Hao , Xue Jijun , Jiang Xianxing
Published: June 2021

Fluorination using chiral catalytic methods could result in a direct access to asymmetric fluorine chemistry. However, challenges in catalytic asymmetric fluorinations, especially the longstanding stereochemical challenges existed in BF3·Et2O-based fluorinations, have not yet been addressed. Here we report the catalytic asymmetric nucleophilic fluorination using BF3·Et2O as the fluorine reagent in the presence of chiral iodine catalyst. Various chiral fluorinated oxazine products were obtained with good to excellent enantioselectivities (up to >99% ee) and diastereoselectivities (up to >20:1 dr). Control experiments (the desired fluoro-oxazines could not be obtained when Py·HF or Et3N·3HF were employed as the fluorine source) indicated that BF3·Et2O acted not only as a fluorine reagent but also as the activating reagent for activation of iodosylbenzene.
Population genomics of apricots unravels domestication history and adaptive events
Authors: Groppi Alexis , Liu Shuo , Cornille Amandine , Decroocq Stéphane , Bui Quynh Trang , Tricon David , Cruaud Corinne , Arribat Sandrine , Belser Caroline , Marande William , Salse Jérôme , Huneau Cécile , Rodde Nathalie , Rhalloussi Wassim , Cauet Stéphane , Istace Benjamin , Denis Erwan , Carrère Sébastien , Audergon Jean-Marc , Roch Guillaume , Lambert Patrick , Zhebentyayeva Tetyana , Liu Wei-Sheng , Bouchez Olivier , Lopez-Roques Céline , Serre Rémy-Félix , Debuchy Robert , Tran Joseph , Wincker Patrick , Chen Xilong , Pétriacq Pierre , Barre Aurélien , Nikolski Macha , Aury Jean-Marc , Abbott Albert Glenn , Giraud Tatiana , Decroocq Véronique
Published: June 2021

Among crop fruit trees, the apricot (Prunus armeniaca) provides an excellent model to study divergence and adaptation processes. Here, we obtain nearly 600 Armeniaca apricot genomes and four high-quality assemblies anchored on genetic maps. Chinese and European apricots form two differentiated gene pools with high genetic diversity, resulting from independent domestication events from distinct wild Central Asian populations, and with subsequent gene flow. A relatively low proportion of the genome is affected by selection. Different genomic regions show footprints of selection in European and Chinese cultivated apricots, despite convergent phenotypic traits, with predicted functions in both groups involved in the perennial life cycle, fruit quality and disease resistance. Selection footprints appear more abundant in European apricots, with a hotspot on chromosome 4, while admixture is more pervasive in Chinese cultivated apricots. Our study provides clues to the biology of selected traits and targets for fruit tree research and breeding.
GFAP and desmin expression in lymphatic tissues leads to difficulties in distinguishing between glial and stromal cells
Authors: Günther Hauke Simon , Henne Stephan , Oehlmann Jasmin , Urban Julia , Pleizier Desiree , Renevier Niclas , Lohr Christian , Wülfing Clemens
Published: June 2021

Recently, we found many immune cells including antigen presenting cells neurally hard wired in the T-cell zone of most lymphoid organs like amongst others, lymph nodes in rats, mice and humans. Single immune cells were reached by single neurites and enclosed with a dense neural meshwork. As it is well known that axons are always accompanied by glial cells, we were able to identify Schwann cells in the hilum, medullary and capsule region, like expected. Unexpected was the result, that we found oligodendrocyte-like cells in these regions, myelinating more than one axon. Likewise important was the finding, that one of the standard glial markers used, a polyclonal GFAP antibody equally bound to desmin and therefore marked nearly all stromal cells in cortical, paracortical and medullary cord regions. More detailed analysis showed that these results also appeared in many other non-lymphoid organs. Therefore, polyclonal GFAP antibodies are only conditionally usable for immunohistochemical analysis in peripheral tissues outside the central nervous system. It remains to be elucidated, if the binding of the GFAP antibody to desmin has its reason in a special desmin variant that can give stromal cells glial character.
Simultaneous multi-nuclide imaging via double-photon coincidence method with parallel hole collimators
Authors: Uenomachi Mizuki , Shimazoe Kenji , Ogane Kenichiro , Takahashi Hiroyuki
Published: June 2021

Multi-tracer imaging can provide useful information in the definitive diagnosis and research of medical, biological, and pharmaceutical sciences. Single-photon emission computed tomography (SPECT) is one of the nuclear medicine imaging modalities widely used for diagnosis or medical research and has a multi-tracer imaging capability. One of the drawbacks of multi-tracer imaging is crosstalk from other gamma rays, which affects the reconstructed image. Scattering correction methods, such as the dual- and triple-energy window methods, are used for conventional SPECT imaging to reduce the background caused by the crosstalk. This study proposes another crosstalk reduction method. Some nuclides emit two or more gamma rays through intermediate levels. Thus, detecting these gamma rays with the coincidence method allows us to distinguish a true gamma ray signal and a background signal. The nuclide position can be estimated at the intersection of two gamma rays using collimators. We demonstrate herein simultaneous 111In and 177Lu imaging via the double-photon coincidence method using GAGG detectors and parallel hole collimators. The double-photon coincidence method greatly reduces the background caused by other gamma rays and offers higher-quality images than does conventional imaging.
Determinants of moult haulout phenology and duration in southern elephant seals
Authors: de Kock Leandri , Oosthuizen W. Chris , Beltran Roxanne S. , Bester Marthán N. , de Bruyn P. J. Nico
Published: June 2021

Phenological shifts are among the most obvious biological responses to environmental change, yet documented responses for Southern Ocean marine mammals are extremely rare. Marine mammals can respond to environmental changes through phenological flexibility of their life-history events such as breeding and moulting. Southern elephant seals (Mirounga leonina) undergo an obligatory annual moult which involves the rapid shedding of epidermal skin and hair while seals fast ashore. We quantified the timing (phenology) and duration (the time from arrival ashore to departure) of the moult haulout of 4612 female elephant seals at Marion Island over 32 years. Using linear mixed-effects models, we investigated age, breeding state and environmental drivers of moult timing and haulout duration. We found no clear evidence for a temporal shift in moult phenology or its duration. Annual variation in moult arrival date and haulout duration was small relative to age and breeding effects, which explained more than 90% of the variance in moult arrival date and 25% in moult haulout duration. All environmental covariates we tested explained minimal variation in the data. Female elephant seals moulted progressively later as juveniles, but adults age 4 and older had similar moult start dates that depended on the breeding state of the female. In contrast, moult haulout duration was not constant with age among adults, but instead became shorter with increasing age. Moulting is energetically expensive and differences in the moult haulout duration are possibly due to individual variation in body mass and associated metabolizable energy reserves, although other drivers (e.g. hormones) may also be present. Individual-based data on moult arrival dates and haulout duration can be used as auxiliary data in demographic modelling and may be useful proxies of other important biological parameters such as body condition and breeding history.
Implication of problematic substance use in poststroke depression: an hospital-based study
Authors: Rabat Yolaine , Sibon Igor , Berthoz Sylvie
Published: June 2021

The prevalence of clinically defined problematic substance use among stroke patients is overlooked and its association with post-stroke depression (PSD) is unknown. Our aims were to: (1) estimate the proportion of stroke patients with a problematic substance use as defined by clinical screening scales; (2) determine the proportion of PSD at three months of follow-up; (3) explore if the baseline severity in substance use and its evolution are independent predictors of PSD. A cohort of first-ever non-severe stroke adult patients was screened at baseline and three months post-stroke using recommended cut-off scores of standardized scales for tobacco, alcohol and cannabis abuse. PSD was defined using the Center of Epidemiological Studies Depression scale score. Out of the 244 eligible patients, 74 (30.3%) presented a problematic substance use, including 21 (8.6%) polydrug abusers. Among these patients, the prevalence of PSD was 50.8%, including 29.5% of severe depression. The severity of tobacco dependence at baseline was found to double the risk (OR 1.59, 95% CI 1.05–2.43) of presenting a PSD, independently of previously reported risk factors. We found no significant evidence for an effect of the evolution in substance use at follow-up. Addictive disorders are part of the critical unmet needs that should be addressed in the management of PSD.
Mesna ameliorates acute lung injury induced by intestinal ischemia–reperfusion in rats
Authors: Abd El-Baset Samia Adel , Abd El-haleem Manal R. , Abdul-Maksoud Rehab S. , Kattaia Asmaa A. A.
Published: June 2021

The lung is severely affected by intestinal ischemia–reperfusion (I–R) injury. Mesna, a thiol compound, possess anti-inflammatory and antioxidant properties. We aimed in the present work to explore the potential beneficial effects of Mesna on the acute lung damage mediated by intestinal I–R in a rat model. Forty male adult albino rats were randomly separated into; control, intestinal I–R, Mesna I and Mesna II groups. Mesna was administered by intraperitoneal injection at a dose of 100 mg/kg, 60 min before ischemia (Mesna I) and after reperfusion (Mesna II). Arterial blood gases and total proteins in bronchoalveolar lavage (BAL) were measured. Lung tissue homogenates were utilized for biochemical assays of proinflammatory cytokines and oxidative stress markers. Lung specimens were managed for examination by light and electron microscopy. Our results revealed that Mesna attenuated the histopathological changes and apoptosis of the lung following intestinal I–R. Mesna also recovered systemic oxygenation. Mesna suppressed neutrophil infiltration (as endorsed by the reduction in MPO level), reduced ICAM-1 mRNA expression, inhibited NF-κB pathway and reduced the proinflammatory cytokines (TNF-α, IL-1β and IL-6) in the lung tissues. Mesna maintained the antioxidant profile as evidenced by the elevation of the tissue GPx and SOD and down-regulation of HSP70 immune-expressions. Accordingly, Mesna treatment can be a promising way to counteract remote injury of the lung resulted from intestinal I–R.
The paradox of second-order homophily in networks
Authors: Evtushenko Anna , Kleinberg Jon
Published: June 2021

Homophily—the tendency of nodes to connect to others of the same type—is a central issue in the study of networks. Here we take a local view of homophily, defining notions of first-order homophily of a node (its individual tendency to link to similar others) and second-order homophily of a node (the aggregate first-order homophily of its neighbors). Through this view, we find a surprising result for homophily values that applies with only minimal assumptions on the graph topology. It can be phrased most simply as “in a graph of red and blue nodes, red friends of red nodes are on average more homophilous than red friends of blue nodes”. This gap in averages defies simple intuitive explanations, applies to globally heterophilous and homophilous networks and is reminiscent of but structually distinct from the Friendship Paradox. The existence of this gap suggests intrinsic biases in homophily measurements between groups, and hence is relevant to empirical studies of homophily in networks.
Chances and challenges of a long-term data repository in multiple sclerosis: 20th birthday of the German MS registry
Authors: Ohle Lisa-Marie , Ellenberger David , Flachenecker Peter , Friede Tim , Haas Judith , Hellwig Kerstin , Parciak Tina , Warnke Clemens , Paul Friedemann , Zettl Uwe K. , Stahmann Alexander
Published: June 2021

In 2001, the German Multiple Sclerosis Society, facing lack of data, founded the German MS Registry (GMSR) as a long-term data repository for MS healthcare research. By the establishment of a network of participating neurological centres of different healthcare sectors across Germany, GMSR provides observational real-world data on long-term disease progression, sociodemographic factors, treatment and the healthcare status of people with MS. This paper aims to illustrate the framework of the GMSR. Structure, design and data quality processes as well as collaborations of the GMSR are presented. The registry’s dataset, status and results are discussed. As of 08 January 2021, 187 centres from different healthcare sectors participate in the GMSR. Following its infrastructure and dataset specification upgrades in 2014, more than 196,000 visits have been recorded relating to more than 33,000 persons with MS (PwMS). The GMSR enables monitoring of PwMS in Germany, supports scientific research projects, and collaborates with national and international MS data repositories and initiatives. With its recent pharmacovigilance extension, it aligns with EMA recommendations and helps to ensure early detection of therapy-related safety signals.
Pulmonary adverse drug event data in hypertension with implications on COVID-19 morbidity
Authors: Jaberi-Douraki Majid , Meyer Emma , Riviere Jim , Gedara Nuwan Indika Millagaha , Kawakami Jessica , Wyckoff Gerald J. , Xu Xuan
Published: June 2021

Hypertension is a recognized comorbidity for COVID-19. The association of antihypertensive medications with outcomes in patients with hypertension is not fully described. However, angiotensin-converting enzyme 2 (ACE2), responsible for host entry of the novel coronavirus (SARS-CoV-2) leading to COVID-19, is postulated to be upregulated in patients taking angiotensin-converting enzyme inhibitors (ACEIs) and angiotensin II receptor blockers (ARBs). Here, we evaluated the occurrence of pulmonary adverse drug events (ADEs) in patients with hypertension receiving ACEIs/ARBs to determine if disparities exist between individual drugs within the respective classes using data from the FDA Spontaneous Reporting Systems. For this purpose, we proposed the proportional reporting ratio to provide a statistical summary for the commonality of an ADE for a specific drug as compared to the entire database for drugs in the same or other classes. In addition, a statistical procedure, multiple logistic regression analysis, was employed to correct hidden confounders when causative covariates are underreported or untrusted to correct analyses of drug-ADE combinations. To date, analyses have been focused on drug classes rather than individual drugs which may have different ADE profiles depending on the underlying diseases present. A retrospective analysis of thirteen pulmonary ADEs showed significant differences associated with quinapril and trandolapril, compared to other ACEIs and ARBs. Specifically, quinapril and trandolapril were found to have a statistically significantly higher incidence of pulmonary ADEs compared with other ACEIs as well as ARBs (P < 0.0001) for group comparison (i.e., ACEIs vs. ARBs vs. quinapril vs. trandolapril) and (P ≤ 0.0007) for pairwise comparison (i.e., ACEIs vs. quinapril, ACEIs vs. trandolapril, ARBs vs. quinapril, or ARBs vs. trandolapril). This study suggests that specific members of the ACEI antihypertensive class (quinapril and trandolapril) have a significantly higher cluster of pulmonary ADEs.