bluuebunny/crossref_metadata_2025 huggingface dataset:
Virucidal effects of eucalyptus essential oil on porcine reproductive and respiratory syndrome virus
Authors: Chen Tianbao , Liu Baoling , Liu Dingyu , Luo Qin , Wang Yani , Wang Xiaohu , He Dongsheng , Cai Rujian
Published: August 2024

Currently, the efficacy of vaccination for preventing and controlling PRRSV is insufficient. Therefore, there is an urgent need for novel effective preventive strategies. This study aimed to investigate the antiviral effect of Eucalyptus essential oil (EEO) against PRRSV in vitro. Marc-145 cells were infected with PRRSV (rJXA1-R), and the toxicity of EEO in the cells was measured using the Cell Counting Kit-8 method. Additionally, the antiviral effect of EEO on PRRSV-infected cells was assessed using three treatment methods: drug administration post-PRRSV inoculation (post-treatment), drug administration before PRRSV inoculation (pre-treatment), and simultaneous drug administration and PRRSV inoculation (co-treatment). The EEO could not inhibit virus adsorption and/or replication since post-treatment and pre-treatment did not prevent viral infectivity. However, EEO exerted a significant virucidal effect on PRRSV. When PRRSV-infected cells were treated with 0.0156, 0.0312, and 0.0625% EEO, the cell survival rates were 55.37, 118.96, and 121.67%, respectively, and the titer of progeny virions decreased from 5.77 Log10TCID50 to 5.21 Log10TCID50, 0.55 Log10TCID50, and less than 0.167 Log10TCID50, respectively (where TCID50 is the 50% tissue culture infected dose). The fluorescence intensity of the PRRSV N protein significantly decreased in the indirect immunofluorescence assay. When cells were co-treated with EEO (0.0625%) and PRRSV (1000 TCID50) for 15 min, the viral particles were inactivated, and PRRSV (1000 TCID50) particles loss infectivity when the co-treatment time reached 60 min. In a word, EEO has no obvious therapeutic effect on PRRSV infection, but it can effectively inactivate virus particles and make them lose the ability to infect cells. These findings provide insights for the development and use of EEO to treat PRRS.
Soil microbial community composition and nitrogen enrichment responses to the operation of electric power substation
Authors: Xu Zhi-Xin , Zeng Bo , Chen Sheng , Xiao Sa , Jiang Lin-Gao , Li Xiang , Wu Yun-Fang , You Le-Xing
Published: August 2024

The surge in global energy demand mandates a significant expansion of electric power substations. Nevertheless, the ecological consequences of electric power substation operation, particularly concerning the electromagnetic field, on soil microbial communities and nitrogen enrichment remain unexplored. In this study, we collected soil samples from six distinct sites at varying distances from an electric power substation in Xintang village, southeastern China, and investigated the impacts of electromagnetic field on the microbial diversity and community structures employing metagenomic sequencing technique. Our results showed discernible dissimilarities in the fungal community across the six distinct sites, each characterized by unique magnetic and electric intensities, whereas comparable variations were not evident within bacterial communities. Correlation analysis revealed a diminished nitrogen fixation capacity at the site nearest to the substation, characterized by low moisture content, elevated pH, and robust magnetic induction intensity and electric field intensity. Conversely, heightened nitrification processes were observed at this location compared to others. These findings were substantiated by the relative abundance of key genes associated with ammonium nitrogen and nitrate nitrogen production. This study provides insights into the relationships between soil microbial communities and the enduring operation of electric power substations, thereby contributing fundamental information essential for the rigorous environmental impact assessments of these facilities.
Oleuropein mediated autophagy begets antimalarial drug resistance
Authors: Sharma Prakriti , Chaudhary Neil Roy , Devi Sonia , Negi Sushmita , Tandel Nikunj , Tyagi Rajeev K.
Published: August 2024

Drug resistance in Plasmodium falciparum presents a formidable challenge to the humanity. And, unavailability of an effective vaccine worsens the situation further. Autophagy is one of the mechanisms employed by parasite to evade drug pressure to survive. Autophagy induced by the P. falciparum in response to the oleuropein pressure may answer many questions related to the parasite survival as well as evolving drug tolerance. The survival/autophagy axis could be an important avenue to explore in order to address certain questions related to the evolution of drug resistance. In addition, humanized mouse model of P. falciparum infection could serve as an important preclinical tool to investigate the oleuropein-induced autophagy, potentially helping to dissect the mechanisms underlying the development of antimalarial drug resistance.
Molecular identification of rhamnolipids produced by Pseudomonas oryzihabitans during biodegradation of crude oil
Authors: Hosseini Saman , Sharifi Rouhallah , Habibi Alireza , Ali Qurban
Published: August 2024

Introduction

The ability to produce biosurfactants plays a meaningful role in the bioavailability of crude oil hydrocarbons and the bioremediation efficiency of crude oil-degrading bacteria. This study aimed to characterize the produced biosurfactants by Pseudomonas oryzihabitans during the biodegradation of crude oil hydrocarbons.

Methods

The biosurfactants were isolated and then characterized by Fourier transform infrared (FTIR), liquid chromatography-mass-spectrometry (LC–MS), and nuclear magnetic resonance spectroscopy (NMR) analyses.

Results

The FTIR results revealed the existence of hydroxyl, carboxyl, and methoxyl groups in the isolated biosurfactants. Also, the LC–MS analysis demonstrated a main di-rhamnolipid (l-rhamnopyranosyll-rhamnopyranosyl-3-hydroxydecanoyl-3-hydroxydecanoate, Rha-Rha-C10-C10) along with a mono-rhamnolipid (l-rhamnopyranosyl-b-hydroxydecanoylb-hydroxydecanoate, Rha-C10-C10). In agreement with these findings, the NMR analysis confirmed the aromatic, carboxylic, methyl, sulfate moieties, and hexose sugar in the biosurfactants. The emulsion capacity of the biosurfactants decreased the surface tension of the aqueous system from 73.4 mN m−1 to around 33 mN m−1 at 200 mg L−1 as the critical micelle concentration. The emulsification capacity of the biosurfactants in the formation of a stable microemulsion for the diesel-water system at a wide range of pH (2–12), temperature (0–80°C), and salinity (2–20 g L−1 of NaCl) showed their potential use in oil recovery and bioremediation through the use of microbial enhancement.

Discussion

This work showed the ability of Pseudomonas oryzihabitans NC392 cells to produce rhamnolipid molecules during the biodegradation process of crude oil hydrocarbons. These biosurfactants have potential in bioremediation studies as eco-friendly and biodegradable products, and their stability makes them optimal for areas with extreme conditions.
PBMC-engrafted humanized mice models for evaluating immune-related and anticancer drug delivery systems
Authors: Kametani Yoshie , Ito Ryoji , Manabe Yoshiyuki , Kulski Jerzy K. , Seki Toshiro , Ishimoto Hitoshi , Shiina Takashi
Published: August 2024

Immune-related drug delivery systems (DDSs) in humanized mouse models are at the forefront of cancer research and serve as bridges between preclinical studies and clinical applications. These systems offer unique platforms for exploring new therapies and understanding their interactions with human cells and the immune system. Here, we focus on a DDS and a peripheral blood mononuclear cell (PBMC)-engrafted humanized mouse model that we recently developed, and consider some of the key components, challenges, and applications to advance these systems towards better cancer treatment on the basis of a better understanding of the immune response. Our DDS is unique and has a dual function, an anticancer effect and a capacity to fine-tune the immune reaction. The PBL-NOG-hIL-4-Tg mouse system is superior to other available humanized mouse systems for the development of such multifunctional DDSs because it supports the rapid reconstruction of an individual donor’s immunity and avoids the onset of graft-versus-host disease.
Classification of Parkinson’s disease by deep learning on midbrain MRI
Authors: Welton Thomas , Hartono Septian , Lee Weiling , Teh Peik Yen , Hou Wenlu , Chen Robert Chun , Chen Celeste , Lim Ee Wei , Prakash Kumar M. , Tan Louis C. S. , Tan Eng King , Chan Ling Ling
Published: August 2024

Purpose

Susceptibility map weighted imaging (SMWI), based on quantitative susceptibility mapping (QSM), allows accurate nigrosome-1 (N1) evaluation and has been used to develop Parkinson’s disease (PD) deep learning (DL) classification algorithms. Neuromelanin-sensitive (NMS) MRI could improve automated quantitative N1 analysis by revealing neuromelanin content. This study aimed to compare classification performance of four approaches to PD diagnosis: (1) N1 quantitative “QSM-NMS” composite marker, (2) DL model for N1 morphological abnormality using SMWI (“Heuron IPD”), (3) DL model for N1 volume using SMWI (“Heuron NI”), and (4) N1 SMWI neuroradiological evaluation.

Method

PD patients (n = 82; aged 65 ± 9 years; 68% male) and healthy-controls (n = 107; 66 ± 7 years; 48% male) underwent 3 T midbrain MRI with T2*-SWI multi-echo-GRE (for QSM and SMWI), and NMS-MRI. AUC was used to compare diagnostic performance. We tested for correlation of each imaging measure with clinical parameters (severity, duration and levodopa dosing) by Spearman-Rho or Kendall-Tao-Beta correlation.

Results

Classification performance was excellent for the QSM-NMS composite marker (AUC = 0.94), N1 SMWI abnormality (AUC = 0.92), N1 SMWI volume (AUC = 0.90), and neuroradiologist (AUC = 0.98). Reasons for misclassification were right–left asymmetry, through-plane re-slicing, pulsation artefacts, and thin N1. In the two DL models, all 18/189 (9.5%) cases misclassified by Heuron IPD were controls with normal N1 volumes. We found significant correlation of the SN QSM-NMS composite measure with levodopa dosing (rho = −0.303, p = 0.006).

Conclusion

Our data demonstrate excellent performance of a quantitative QSM-NMS marker and automated DL PD classification algorithms based on midbrain MRI, while suggesting potential further improvements. Clinical utility is supported but requires validation in earlier stage PD cohorts.
A single intravenous reelin injection restores corticosterone-induced neurochemical and behavioral alterations in dams during the post-partum period
Authors: Sánchez-Lafuente Carla L. , Johnston Jenessa N. , Reive Brady S. , Scheil Kaylene K. A. , Halvorson Ciara S. , Jimenez Mariana , Colpitts Darian , Kalynchuk Lisa E. , Caruncho Hector J.
Published: August 2024

Introduction

Treatment with the synaptic plasticity protein reelin has rapid antidepressant-like effects in adult corticosterone (CORT)-induced depressed rats, whether administered repeatedly or acutely. However, these effects remain unexplored in the context of post-partum depression (PPD).

Methods

This study investigated the antidepressant-like effect of a single injection of reelin in a CORT-induced model of PPD. Long-Evans female dams received either daily subcutaneous CORT (40 mg/kg) or saline injections (controls) from the post-partum day (PD) 2 to 22, and on PD22 were treated with a single intravenous reelin (3 μg) or vehicle injection.

Results

Reelin treatment fully normalized to control levels the CORT-induced increase in Forced Swim Test (FST) immobility and the decrease in reelin-positive cells in the subgranular zone of the intermediate hippocampus. It also increased the number of oxytocin-positive cells in the paraventricular nucleus (PVN), the number of reelin-positive cells in the dorsal and ventral hippocampus, and the dendritic complexity of newborn neurons in the intermediate hippocampus, causing a partial recovery compared to controls. None of these changes were associated with fluctuations in estrogen levels measured peripherally.

Discussion

This study brings new insights into the putative antidepressant-like effect of peripherally administered reelin in an animal model of PPD. Future studies should be conducted to investigate these effects on a dose–response paradigm and to further elucidate the mechanisms underlying the antidepressant-like effects of reelin.
Association between dietary vitamin E intake and constipation: NHANES 2005–2010
Authors: Cai Junfeng , Li Danqing , Xie Ruijun , Yu Xiaoling , Wu Yuning , Sun Feng , Zhang Chenxiong
Published: August 2024

Background

This investigation aimed to analyze the association between dietary vitamin E intake and constipation prevalence among United States adults.

Methods

Utilizing data from the National Health and Nutrition Examination Survey (NHANES), this cross-sectional study assessed vitamin E intake through 24-h dietary recall and defined constipation based on the Bristol Stool Form Scale (BSFS). Logistic regression models were employed to evaluate the relationship between vitamin E intake and constipation, with results presented as odds ratios (ORs) and 95% confidence intervals (CIs). Stratified analyses were conducted based on covariates such as age, and restricted cubic spline (RCS) models were generated to explore the potential linear or non-linear association.

Results

Individuals experiencing constipation exhibited lower vitamin E intake compared to those without constipation. Weighted multivariate logistic regression models demonstrated a negative correlation between vitamin E intake and constipation risk, even after adjusting for potential confounding variables. Further RCS analysis revealed a statistically significant non-linear inverse relationship between vitamin E intake and constipation risk (p-value for non-linearity = 0.0473).

Conclusion

Our findings suggest an independent inverse association between vitamin E intake and constipation prevalence in United States adults. Prospective research is needed to validate these observations.
Case Report: p40phox deficiency underlying pediatric-onset systemic lupus erythematosus
Authors: Nieto-Patlán Alejandro , Fernández Dávila Natalia S. , Wang Yuqing , Zelnick Michelle , Muscal Eyal , Curry Martha , Lupski James R. , Holland Steven M. , Yuan Bo , Kuhns Douglas B. , Vogel Tiphanie P. , Chinn Ivan K.
Published: August 2024

Introduction

Systemic lupus erythematosus is a multi-faceted autoimmune disorder of complex etiology. Pre-pubertal onset of pediatric systemic lupus erythematosus (pSLE) is uncommon and should raise suspicion for a genetic driver of disease. Autosomal recessive p40phox deficiency is a rare immunologic disorder characterized by defective but not abolished NADPH oxidase activity with residual production of reactive oxygen species (ROS) by phagocytic cells.

Case presentation

We report the case of a now 18-year-old female with pSLE onset at 7 years of age. She presented with recurrent fever and malar rash. Aspects of her immune dysregulation over time have included typical pSLE features including production of autoantibodies, hematologic manifestations, and hypocomplementemia, as well as chronic suppurative skin lesions and recurrent infections. Genetic analysis revealed biallelic pathogenic variants in NCF4 resulting in p40phox deficiency. Comprehensive NADPH oxidase activity studies confirmed significantly decreased production of reactive oxygen species, confirming the cellular phenotype seen in p40phox deficient patients.

Conclusions

Here, we present a patient with pSLE harboring biallelic variants in NCF4. Our patient represents a unique clinical presentation of severe onset autoimmunity in the setting of a rare inborn error of immunity affecting NADPH oxidase activity. This case underscores the need to consider genetic causes of pSLE in cases of pre-pubertal onset and atypical disease.
Effects of attentional focus strategies in drop landing biomechanics of individuals with unilateral functional ankle instability
Authors: Wang Zilong , Meng Lingyue , Lu Mengya , Kong Lingyu , Xue Jingxian , Zhang Zhiqi , Meng Xin , Zhang Qiuxia
Published: August 2024

Background

Functional Ankle Instability (FAI) is a pervasive condition that can emerge following inadequate management of lateral ankle sprains. It is hallmarked by chronic joint instability and a subsequent deterioration in physical performance. The modulation of motor patterns through attentional focus is a well-established concept in the realm of motor learning and performance optimization. However, the precise manner in which attentional focus can rehabilitate or refine movement patterns in individuals with FAI remains to be fully elucidated.

Objective

The primary aim of this study was to evaluate the impact of attentional focus strategies on the biomechanics of single-leg drop landing movements among individuals with FAI.

Methods

Eighteen males with unilateral FAI were recruited. Kinematic and kinetic data were collected using an infrared three-dimensional motion capture system and force plates. Participants performed single-leg drop landing tasks under no focus (baseline), internal focus (IF), and external focus (EF) conditions. Biomechanical characteristics, including joint angles, ground reaction forces, and leg stiffness, were assessed. A 2 × 3 [side (unstable and stable) × focus (baseline, IF, and EF)] Repeated Measures Analysis of Variance (RM-ANOVA) analyzed the effects of attentional focus on biomechanical variables in individuals with FAI.

Results

No significant interaction effects were observed in this study. At peak vertical ground reaction force (vGRF), the knee flexion angle was significantly influenced by attentional focus, with a markedly greater angle under EF compared to IF (p < 0.001). Additionally, at peak vGRF, the ankle joint plantarflexion angle was significantly smaller with EF than with IF (p < 0.001). Significant main effects of focus were found for peak vGRF and the time to reach peak vGRF, with higher peak vGRF values observed under baseline and IF conditions compared to EF (p < 0.001). Participants reached peak vGRF more quickly under IF (p < 0.001). Leg Stiffness (kleg) was significantly higher under IF compared to EF (p = 0.001).

Conclusion

IF enhances joint stability in FAI, whereas EF promotes a conservative landing strategy with increased knee flexion, dispersing impact and minimizing joint stress. Integrating these strategies into FAI rehabilitation programs can optimize lower limb biomechanics and reduce the risk of reinjury.
Effects of Streptomyces melanosporofaciens X216 on microbial diversity in oilseed rape soil
Authors: Liang Hai-di , Zhou Hu , Zhao Hui , Ding Lin , Zhou Jia , Zhang Ying-jun , Gao Yang , Ren Zuo-hua
Published: August 2024

Introduction

Clubroot disease is a devastating soil borne disease caused by infection with Plasmodiophora brassicae, which primarily affects cruciferous plants. The microbial diversity of the soil is an essential indicator of its quality.

Methods

This study measured the physicochemical properties of the soil to study the effect of its microbial diversity on the infection of oilseed rape with P. brassicae. High-throughput sequences of the soil bacteria and fungi in the inter-root soils of P. brassicae were analyzed under different treatment conditions.

Results

In the study, it was found that the efficiency of strain X216 in preventing and controlling the root disease of rapeseed was positively correlated with the amount of solution used to irrigate the root system. The results of the greenhouse and field trials showed that the efficiency of strain X216 against the root disease of rapeseed was 43.16% in the field and 62.14% in the greenhouse. Proteobacteria, Chloroflexi, Rozellomycota, and Basidiomycota are critical phylum in the development of clubroot disease. The application of biocontrol increased the relative abundance of Actinobacteria, Bacillus, Mesorhizobium, Mycobacterium, Streptomyces and Filobasidium, which affected the structure and abundance of microbial communities. A principal coordinate analysis showed that the microbial structure in the soil varied substantially in the bacterial community, and there was no significant difference in soil structure in the fungal community.

Discussion

The occurrence of clubroot disease affected the structure of inter-root microbial community composition in the soil, which resulted in a decrease in its community diversity. The application of the biocontrol bacterium X216 increased the soil microbial diversity. It effectively reduced the occurrence of P. brassicae, and this study provides a basis to study the microbial diversity in cruciferous crops.
Comprehensive analysis of the Lycopodium japonicum mitogenome reveals abundant tRNA genes and cis-spliced introns in Lycopodiaceae species
Authors: Sun Ning , Han Fuchuan , Wang Suyan , Shen Fei , Liu Wei , Fan Weishu , Bi Changwei
Published: August 2024

Lycophytes and ferns represent one of the earliest-diverging lineages of vascular plants, with the Lycopodiaceae family constituting the basal clade among lycophytes. In this research, we successfully assembled and annotated the complete Lycopodium japonicum Thunb. (L. japonicum) mitochondrial genome (mitogenome) utilizing PacBio HiFi sequencing data, resulting in a single circular molecule with a size of 454,458 bp. 64 unique genes were annotated altogether, including 34 protein-coding genes, 27 tRNAs and 3 rRNAs. It also contains 32 group II introns, all of which undergo cis-splicing. We identified 195 simple sequence repeats, 1,948 dispersed repeats, and 92 tandem repeats in the L. japonicum mitogenome. Collinear analysis indicated that the mitogenomes of Lycopodiaceae are remarkably conserved compared to those of other vascular plants. We totally identified 326 RNA editing sites in 31 unique protein-coding genes with 299 sites converting cytosine to uracil and 27 sites the reverse. Notably, the L. japonicum mitogenome has small amounts foreign DNA from plastid or nuclear origin, accounting for only 2.81% of the mitogenome. The maximum likelihood phylogenetic analysis based on 23 diverse land plant mitogenomes and plastid genomes supports the basal position of lycophytes within vascular plants and they form a sister clade to all other vascular lineages, which is consistent with the PPG I classification system. As the first reported mitogenome of Lycopodioideae subfamily, this study enriches our understanding of Lycopodium mitogenomes, and sets the stage for future research on mitochondrial diversity and evolution within the lycophytes and ferns.
Association between cognitive insight and anxiety among community-dwelling schizophrenia patients: the chain mediating effects of family cohesion and chronotype
Authors: Dong Meijun , Wu Dongmei , Mao Guoju , Yan Zhangrong
Published: August 2024

Objective

To investigate the effect of cognitive insight on anxiety in community-dwelling schizophrenia patients and to construct a chain mediating model to determine the underlying mechanisms of the relationship between cognitive insight and anxiety through family cohesion and chronotype.

Methods

The Beck Cognitive Insight Scale, the Chinese version of the Family Adaptability and Cohesion Scale, the Morningness-Eveningness Scale, and the Generalized Anxiety Disorder Scale were used to assess cognitive insight, family cohesion, chronotype, and anxiety in a sample of 785 individuals with schizophrenia living in the community. Spearman correlation analysis, multiple linear regression and Bootstrap methods were employed to analyze the four variables.

Results

Residential region, current employment status, and family economic status impacted anxiety among community-dwelling schizophrenia patients. All variables were associated with each other, but self-certainty was not significantly associated with anxiety. Cognitive insight and self-reflection had direct effects on anxiety, as well as indirect effects on anxiety through the partial mediating effect of chronotype, the suppressing effect of family cohesion, and the chain mediating effect of family cohesion and chronotype.

Conclusions

Family cohesion and chronotype can mediate the relationship between cognitive insight and anxiety. Improving family functioning, promoting emotional relationships within families, and correcting non-circadian sleep patterns can reduce anxiety in community-dwelling schizophrenia patients, with important implications for improving adverse mental health outcomes.
Co-occurring autism, ADHD, and gender dysphoria in children, adolescents, and young adults with eating disorders: an examination of pre- vs. post-COVID pandemic outbreak trends with real-time electronic health record data
Authors: Brown Tashalee R. , Jansen Madeline O. , Zhou A. Ning , Moog Dominic , Xie Hui , Liebesny Katherine V. , Xu Kevin Y. , Lin Binx Y. , Deng Wisteria Y.
Published: August 2024

Background

Incidence rates of autism, attention-deficit/hyperactivity disorder (ADHD), and gender dysphoria (GD) are rising not only in the general population, but particularly among children, adolescents, and young adults with eating disorders (EDs). While ED rates have risen during the COVID pandemic, trends in co-occurring autism, ADHD, and GD have yet to be investigated in detail or at scale by way of large electronic medical record data.

Objectives

To investigate trends in rates of co-occurring autism, ADHD, and GD among children, adolescents, and young adults with EDs in years prior to and during the COVID-19 pandemic.

Methods

We utilized a de-identified multinational electronic health records database (TriNetX) with 48,558 individuals aged 5-26 diagnosed with eating disorders (EDs) at least twice between 2017 and 2022. The primary predictor variable differentiated between the years of each person’s index (first) ED diagnosis (2017-2019 vs. 2020-2022). The primary outcome variable was the rate of new co-occurring psychiatric diagnoses of autism, ADHD, and GD in the year following each patient’s first ED diagnosis. We applied propensity score-matched multivariable logistic regressions to compare primary outcomes between 2017-2019 and 2020-2022.

Results

Our analysis included 17,445 individuals diagnosed with EDs in 2017-2019 (8% autism, 13.5% ADHD, 1.9% GD) and 31,113 diagnosed with EDs in 2020-2022 (8% autism, 14.6% ADHD, 3.2% GD). After 1:1 propensity score matching, 17,202 individuals from the 2017-2019 cohort were matched to peers mirroring the 2020-2022 cohort. Those diagnosed in 2020-2022 showed a 19% (aOR[95%CI]=1.19[1.07-1.33]), 25% (aOR=1.25[1.04-1.49]), and 36% (aOR=1.36[1.07-1.74]) increase in odds for autism, ADHD, and GD diagnoses, respectively, within the 365 days after the index EDs diagnosis, compared to the 2017-2019 cohort.

Discussion

Rates of autism, ADHD, and GD are significantly higher in individuals with ED in the post-pandemic 2020-2022 cohort in comparison to the pre-pandemic 2017-2019 cohort, even after controlling for baseline levels of co-occurring psychiatric diagnoses. Such findings reveal a critical gap in our current understanding of the totality of ways in which COVID-19 may have impacted the onset and clinical course of EDs, autism, ADHD, and GD among children, adolescents, and young adults.
Hardness changing tactile displays for simulating the feel of organic tissues
Authors: Brown Joshua , Bello Fernando
Published: August 2024

Physical interaction with patients, for example conducted as part of a diagnostic examination or surgical procedure, provides clinicians with a wealth of information about their condition. Simulating this interaction is of great interest to researchers in both haptics and medical education, and the development of softness changing tactile interfaces is important in recreating the feel of different soft tissues. This paper presents designs for a variety of novel electromechanical and electromagnetic mechanisms for controlling particle jamming-based, hardness changing tactile displays, intended to allow medical trainees to experience these physical interactions in a range of simulation settings such as clinical skills teaching laboratories. Each design is then subjected to a battery of mechanical tests to evaluate its effectiveness compared to the state of the art, as well as their suitability for simulating the physical hardness of different types of soft tissues, previously characterised in established literature. These results demonstrate that all of the technologies presented are able to exhibit a measurable hardness change, with Shore hardness values between 3A and 57A achieved by the most effective constriction-based device. The electromechanical devices based on constriction and compression, and the state-of-the-art pneumatic device, were able to achieve hardness changes within a range that is useful for replicating the softness of organic tissue. The electromechanical and electromagnetic devices were also found to effect their full range of hardness change in less than a second, compared to several seconds for the state-of-the-art. These results show that the performance of softness changing tactile displays can be improved with the electromechanical actuation techniques proposed in this paper, and that such displays are able to replicate the physical characteristics of soft tissues and may therefore be of benefit in medical training and simulation scenarios.
Unlocking the nutritional potential: amino acid profile of eight Indian food fishes and their role in meeting recommended dietary allowances
Authors: Das Basanta Kumar , Kumari Kajal , Ganguly Satabdi , Talukder Anjon Kumar , Ray Archisman , Dutta Subhamoy , Baisakhi Barsha
Published: August 2024

Introduction

Proteins and amino acids, as vital biomolecules, are not only part of key metabolic pathways in the body but are also essential for tissue repair, enzyme function, and hormone regulation. They also serve as building blocks for the formation of signaling molecules, such as neurotransmitters and catecholamines. Additionally, amino acids are the primary building components of proteins. Fish serves as a vital source of high-quality animal protein and amino acids, playing a crucial role in supporting human nutrition.

Methods

High-performance liquid chromatography (HPLC) using fluorescence detector was employed to investigated the amino acid content of eight food fishes from diverse aquatic habitats.

Results

The study revealed that the small indigenous fish (SIF) Systomus sarana and the marine fish Sardinella melanura were rich sources of all essential amino acids. Furthermore, estuarine fish like Pisodonophis boro can be recommended for specific amino acids like arginine, histidine, leucine, and valine, while Setipinna phasa is suggested for cysteine.

Discussion

These recommendations rely on the possible contribution of these fishes to the Recommended Dietary Allowance (RDA) regarding each nutrient. The insights gained from this study could be utilized as recommendations to meet amino acid requirement using fish as a natural supplement.
Case Report: A report on the countermeasures after PICC line breakage in 3 postoperative breast cancer patients
Authors: Jing Yuqin , Qu Jing , Jiang Shuo , Wang Xiaomin
Published: August 2024

Peripherally inserted central catheter (PICC) is a widely used technique in oncology chemotherapy, characterized by safety, reliability, and long dwell time. However, a catheter can break due to various causes. When an acute rupture occurs, it always lead to sever complications which may threaten patients’ safety and potentially result in medical disputes. In this study, we collected and analyzed 3 cases of PICC line breakage causing drug leakage in our hospital from 2018 to 2023. All these 3 cases were postoperative breast cancer patients accepting chemotherapy, with 2 cases involving external partial breakage and 1 case involving internal partial breakage. Due to timely and appropriate management, no acute rupture occurred. We propose some ideas such as selecting high-quality catheter materials and avoiding over extension or repeated bending are crucial in preventing PICC line breakage. In addition, we also recommend establishing a standardized and scientific management pattern of PICC to ensure the safety and effectiveness of its clinical application, including comprehensive assessment, “four-element” intervention, and continuous evaluation.
A Data-Driven Comprehensive Evaluation Method for Electromagnetic Suspension Maglev Control System
Authors: Zhou Xingyu , Liang Shi , Li Xiaolong , Long Zhiqiang , Wang Zhiqiang
Published: August 2024

As new advanced vehicles, the safety and stability of electromagnetic suspension maglev trains have always been a subject of concern. This study introduces the improved R index and τ-distance index into the performance evaluation of the suspension control system, respectively assessing the stability of the suspension gap and the smoothness of train operation, combining them with grey relational analysis to achieve data-driven comprehensive evaluation. Furthermore, feasibility tests on the Fenghuang Maglev Express validate the effectiveness and superiority of the comprehensive evaluation method based on measured data. Experimental results demonstrate that the data-driven comprehensive evaluation method, through designing specialized evaluation metrics and increasing assessment dimensions, effectively evaluates the performance of the suspension system control loop. Compared to a traditional error integral comprehensive performance index, it offers greater comprehensiveness and accuracy, along with real-time state-monitoring capabilities.
Design of a Suspension Controller with an Adaptive Feedforward Algorithm for Ride Comfort Enhancement and Motion Sickness Mitigation
Authors: Kim Jinwoo , Yim Seongjin
Published: August 2024

This paper presents a design method of a suspension controller with an adaptive feedforward algorithm for ride comfort enhancement and motion sickness mitigation. Recently, it was shown that motion sickness is caused by combined heave and pitch motions of a sprung mass within the range of 0.8 and 8 Hz. For this reason, it is necessary to design a suspension controller for the purpose of reducing the heave and pitch vibration of a sprung mass within this range. To represent the heave acceleration and the pitch rate of a sprung mass, a 4-DOF half-car model is adopted as a vehicle model. For easy implementation in a real vehicle, a static output feedback control is adopted instead of a full-state one. To reduce the heave acceleration of a sprung mass for ride comfort enhancement, a linear quadratic SOF controller is designed. To reduce the pitch rate of a sprung mass for motion sickness mitigation, a filtered-X LMS algorithm is applied. To validate the method, simulation on vehicle simulation software is conducted. From the simulation results, it is shown that the proposed method is effective for ride comfort enhancement and motion sickness mitigation.
Design and Thermal-Optical Environment Simulation of Double-Slope Greenhouse Roof Structure Based on Ecotect
Authors: Yang Xuanhe , Ma Yunfei
Published: August 2024

At present, most double-slope greenhouse shade systems utilize insulation quilts on a portion of the roof to enhance the light and thermal environment for winter production. However, this method often leads to challenges such as uneven light distribution and significant heat dissipation. To improve the uniformity of light distribution and optimize the light and thermal environment inside a shade room, this study employed Ecotect software 2011 for simulating and analyzing the light and thermal conditions of a double-slope greenhouse shade system. The study aimed to investigate the optimal spacing and width of roof windows. The results demonstrated that a double-slope greenhouse with roof windows of 300 mm width spaced at 3000 mm intervals achieved optimal performance. This configuration yielded a light distribution uniformity of 74.4% and an average temperature of 8.1 °C. Such conditions are conducive to creating an environment suitable for cultivating edible mushrooms, thereby enhancing the quality and consistency of mushroom production. This paper provides an effective method for designing the roof structure of double-slope greenhouses to enhance light capture, thermal regulation, and energy efficiency.
Soil Erosion Characteristics of the Agricultural Terrace Induced by Heavy Rainfalls on Chinese Loess Plateau: A Case Study
Authors: Kang Hongliang , Wang Wenlong , Li Liangna , Han Lei , Wei Sihan
Published: August 2024

Terrace erosion has become increasingly pronounced due to the rising incidence of heavy rainfalls resulting from global climate change; however, the processes and mechanisms governing erosion of loess terraces during such events remain poorly understood. A field investigation was performed following a heavy rainfall event in the Tangjiahe Basin to examine the soil erosion characteristics of loess terraces subjected to heavy rainfall events. The results show that various types of erosion occurred on the terraced fields, including rill, gully, and scour hole in water erosion, and sink hole, collapse, and shallow landslide in gravity erosion. Rill erosion and shallow landslide erosion exhibited the highest frequency of occurrence on the new and old terraces, respectively. The erosion moduli of the gully, scour hole, and sink hole on the new terraces were 171.0%, 119.5%, and 308.7% greater than those on the old terraces, respectively. In contrast, lower moduli of collapse and landslide were observed on the new terraces in comparison to the old terraces, reflecting reductions of 34.2% and 23.4%, respectively. Furthermore, the modulus of water erosion (32,102 t/km2) was 4.5 times that of gravity erosion on the new terraces. Conversely, on the old terrace, the modulus of gravity erosion (8804.1 t/km2) exceeded that of water erosion by 14.5%. Gully erosion and collapse dominated the erosion processes, contributing 67.8% and 9.4% to soil erosion on the new terraces and 38.7% and 34.0%, respectively, on the old terraces. In the study area, the new terraces experienced significantly greater erosion (39,252 t/km2) compared to the old terraces (16,491 t/km2). Plastic film mulching, loose and bare ridges and walls, inclined terrace platforms, and high terrace walls, as well as the developing flow paths, might be the key factors promoting the severe erosion of the terraces during heavy rainfall. Improvements in terrace design, construction technologies, temporary protective measures, agricultural techniques, and management strategies could enhance the prevention of soil erosion on terraces during heavy rainfall events.
African Local Pig Genetic Resources in the Context of Climate Change Adaptation
Authors: Pius Lenox , Huang Shuntao , Wanjala George , Bagi Zoltán , Kusza Szilvia
Published: August 2024

Africa is home to a wide diversity of locally adapted pig breeds whose genetic architecture offers important insights into livestock adaptation to climate change. However, the majority of these inherent traits have not been fully highlighted. This review presents an overview of the current state of African pig genetic resources, providing highlights on their population and production statistics, production system, population diversity indices, and genomic evidence underlying their evolutionary potential. The study results reveal an incomplete characterization of local pig genotypes across the continent. The characterized population, however, demonstrates moderate to high levels of genetic diversity, enough to support breeding and conservation programs. Owing to low genetic differentiation and limited evidence of distinct population structures, it appears that most local pig populations are strains within larger breeds. Genomic evidence has shown a higher number of selection signatures associated with various economically important traits, thus making them potential candidates for climate change adaptation. The reportedly early evidence of hybridization with wild suid groups further suggests untapped insights into disease resistance and resilience traits that need to be illuminated using higher-density markers. Nevertheless, gene introgression from commercial breeds is prevalent across Africa; thus, efforts to realize and utilize these traits must increase before they are permanently depleted.
Book Review: Braastad et al. The Cat: Behaviour and Welfare; CABI: Wallingford, UK, 2022; ISBN: 978-1789242317
Authors: Natoli Eugenia
Published: August 2024

It was challenging to read the entire book The Cat: Behaviour and Welfare by Bjarne O [...]
Effects of a Proprietary Kelp Blend Product on Enteric Methane Production and Tissue Residues in Cattle
Authors: Altman Alexander , Vanzant Eric , Davis Sydney , Harmon David , McLeod Kyle R.
Published: August 2024

Three experiments were performed investigating bovine enteric methane (CH4) production inhibition using a proprietary kelp blend product (PKBP) containing a halogenated methane analog (i.e., bromoform). Calves were fed a corn-silage basal diet top-dressed with the assigned treatment, with rations provided at 1.5 × NEm in Experiments 1 and 2 (n = 12 and 6 steers, respectively) and ad libitum in Experiment 3 (n = 9 steers). In Experiment 1, we evaluated bromoform’s potency in decreasing CH4. Dry matter intake (DMI) was not affected by treatment (p ≥ 0.11; 0 vs. 52.5 ± 10.5 ppm bromoform), whereas bromoform supplementation decreased CH4 (p < 0.01). In Experiments 2 and 3, treatments were 0, 9.5 ± 1.5, or 20 ± 3 ppm bromoform. In Experiment 2, we examined CH4 recovery following bromoform removal from the ration. Bromoform treatments were fed on d1, but not the subsequent 8 d, to investigate residual effects. On d1, CH4 was below limits of detection for 20 ppm bromoform inclusion. Across days, a cubic response (p < 0.01) was observed with 20 ppm bromoform inclusion, but not with 0 and 9.5 ppm inclusion levels. Experiment 3 (30 d finishing trial) tested bromoform effects on feeder calves. DMI (p = 0.53), average daily gain (p = 0.55), and gain:feed (p = 0.82) were not influenced by bromoform inclusion. Bromoform residues were undetectable in liver, kidney, adipose, and muscle samples collected at harvest. These experiments demonstrated that cattle fed PKBP experience short-term reductions in CH4 without tissue accumulation of bromoform and without evidence of effects on animal growth or feed consumption.
Assessment of Captive Environment for Oriental Small-Clawed Otters (Aonyx cinereus) in Otter Cafés in Japan
Authors: Ushine Nana , Kamitaki Ayu , Suzuki Akiyuki , Hayama Shin-Ichi
Published: August 2024

This study investigates the captive environments of oriental small-clawed otter (OSO; Aonyx cinereus) cafés in Japan, aiming to identify discrepancies with established welfare guidelines. Improved management of these commercial facilities could raise awareness about the difficulties of keeping OSOs as pets and enhance their welfare. Utilizing the role of commercial facilities in public outreach, we consider that the rigorous implementation of proper care practices in these establishments could help mitigate the increasing demand for OSOs domestically and contribute to the conservation of the OSO. In this study, we focused on the critical aspects of the captive environment necessary to maintain the psychological well-being of OSOs and evaluated the captive environments of OSOs housed in animal cafés for OSO welfare using non-invasive methods. Based on zoo husbandry standards, it was found that there were deficiencies in aquatic environments and solitary housing conditions; however, the enrichment tools aligned with the guidelines. The results suggest that deviations from the recommended elements in the environment of OSOs in captivity are associated with a tendency of these OSOs to develop various diseases. It is deemed necessary to amend the regulations governing animal-handling businesses to include welfare criteria, and it is considered essential for each establishment to operate only after ensuring sufficient welfare for the OSOs.
Antibiotic Residues and Resistance in Three Wastewater Treatment Plants in Romania
Authors: Polianciuc Svetlana Iuliana , Ciorîță Alexandra , Soran Maria Loredana , Lung Ildiko , Kiss Béla , Ștefan Maria Georgia , Leucuța Daniel Corneliu , Gurzău Anca Elena , Carpa Rahela , Colobațiu Liora Mihaela , Loghin Felicia
Published: August 2024

This study evaluates antibiotic residues and bacterial loads in influent and effluent samples from three wastewater treatment plants (WWTPs) in Romania, across four seasons from 2021 to 2022. Analytical methods included solid-phase extraction and high-performance liquid chromatography (HPLC) to quantify antibiotic concentrations, while microbiological assays estimated bacterial loads and assessed antibiotic resistance patterns. Statistical analyses explored the impact of environmental factors such as temperature and rainfall on antibiotic levels. The results showed significant seasonal variations, with higher antibiotic concentrations in warmer seasons. Antibiotic removal efficiency varied among WWTPs, with some antibiotics being effectively removed and others persisting in the effluent, posing high environmental risks and potential for antibiotic resistance development. Bacterial loads were higher in spring and summer, correlating with increased temperatures. Eight bacterial strains were isolated, with higher resistance during warmer seasons, particularly to amoxicillin and clarithromycin.
Efficacy and Safety of Oral Neomycin for the Decolonization of Carbapenem-Resistant Enterobacterales: An Open-Label Randomized Controlled Trial
Authors: Tancharoen Lalita , Srisomnuek Ananya , Tiengrim Surapee , Thamthaweechok Narisara , Tangkorskul Teerawit , Thamlikitkul Visanu
Published: August 2024

Background: Patients with carbapenem-resistant Enterobacterales (CRE) in the gastrointestinal (GI) tract are at risk for subsequent infections and transmission, necessitating contact precautions. Neomycin has shown in vitro activity against CRE in 66–85% of isolates. This study evaluated the efficacy and safety of neomycin for CRE decolonization. Methods: In this open-label randomized controlled trial, stool/rectal swab samples from high-risk patients were collected and tested for CRE colonization in the GI tract. Patients who had CRE and met eligible criteria were divided into a neomycin group (n = 26; treated with 4.2 g/day neomycin for 5 days) and a control group (n = 26). CRE detection in stool/rectal swabs was performed on days 7 ± 2 and 14 ± 2. Results: The two groups’ baseline characteristics were similar. CRE presence on day 7 ± 2 was significantly lower in the neomycin group (46.2%) than in the control group (80.8%, p = 0.01). Efficacy of neomycin (4.2 g/day for 5 days) for CRE decolonization was 42.8–53.8% by day 7. By day 14 ± 2, the CRE rate in the neomycin group had risen to align with the control group’s rate (73.1% vs. 61.5%, p = 0.56). The neomycin group experienced mild, temporary, gastrointestinal side-effects. Conclusions: Neomycin effectively reduced CRE colonization on day 7 ± 2, but its impact waned by day 14 ± 2. This suggests that neomycin dosage was too low and the duration of treatment was too short for lasting CRE decolonization.