bluuebunny/crossref_metadata_2025 huggingface dataset:
A Study on Affective Dimensions to Engine Acceleration Sound Quality Using Acoustic Parameters
Authors: Moon Soyoun , Park Sunghwan , Park Donggun , Kim Wonjoon , Yun Myung Hwan , Park Dongchul
Published: February 2019

The technical performance of recent automobiles is highly progressed and standardized across different manufacturers. This study seeks to derive a semantic space of engine acceleration sound quality for end users and identify the relation with sound characteristics. For this study, two affective attributes: ‘refined’ and ‘powerful’, and eight acoustic parameters considering revolutions per minute were used to determine the correlation coefficient for those affective attributes. In the experiment, a total of 35 automobiles were selected. Each of the 3rd gear wide open throttle sounds was recorded and evaluated by 42 adult subjects with normal hearing ability and driving license. Their subjective evaluations were analyzed using factor analysis, independent t-test, correlation analysis, and regression analysis. The prediction models for the affective dimensions show distinct differences for the revolutions per minute. From the experiment, it was confirmed that the customers’ affective response can be predicted through the acoustic parameters. In addition, it was found that the initial revolutions per minute in the accelerated condition had the greatest influence on the affective response. This study can be a useful guideline to design engine acceleration sounds that satisfy customers’ affective experience.
Effective Crack Damage Detection Using Multilayer Sparse Feature Representation and Incremental Extreme Learning Machine
Authors: Wang Baoxian , Li Yiqiang , Zhao Weigang , Zhang Zhaoxi , Zhang Yufeng , Wang Zhe
Published: February 2019

Detecting cracks within reinforced concrete is still a challenging problem, owing to the complex disturbances from the background noise. In this work, we advocate a new concrete crack damage detection model, based upon multilayer sparse feature representation and an incremental extreme learning machine (ELM), which has both favorable feature learning and classification capabilities. Specifically, by cropping and using a sliding window operation and image rotation, a large number of crack and non-crack patches are obtained from the collected concrete images. With the existing image patches, the defect region features can be quickly calculated by the multilayer sparse ELM autoencoder networks. Then, the online incremental ELM classified network is used to recognize the crack defect features. Unlike the commonly-used deep learning-based methods, the presented ELM-based crack detection model can be trained efficiently without tediously fine-tuning the entire-network parameters. Moreover, according to the ELM theory, the proposed crack detector works universally for defect feature extraction and detection. In the experiments, when compared with other recently developed crack detectors, the proposed concrete crack detection model can offer outstanding training efficiency and favorable crack detecting accuracy.
Testing Iron Stable Isotope Ratios as a Signature of Biomass Burning
Authors: Kurisu Minako , Takahashi Yoshio
Published: February 2019

Biomass burning is an important source of soluble Fe transported to the open ocean; however, its exact contribution remains unclear. Iron isotope ratios can be used as a tracer because Fe emitted by combustion can yield very low Fe isotope ratios due to isotope fractionation during evaporation processes. However, data on Fe isotope ratios of aerosol particles emitted during biomass burning are lacking. We collected size-fractionated aerosol samples before, during, and after a biomass burning event and compared their Fe isotope ratios. On the basis of the concentrations of several elements and Fe species, Fe emitted during the event mainly comprised suspended soil particles in all the size fractions. Iron isotope ratios of fine particles before and after the event were low due to the influence of other anthropogenic combustion sources, but they were closer to the crustal value during the event because of the influence of Fe from suspended soil. Although Fe isotope ratios of soluble Fe were also measured to reduce Fe from soil components, we did not find low isotope signals. Results suggested that Fe isotope ratios could not identify Fe emitted by biomass burning, and low Fe isotope ratios are found only when the combustion temperature is high enough for a sufficient amount of Fe to evaporate.
Spectral Modeling of Hydrogen Radiation Emission in Magnetic Fusion Plasmas
Authors: Koubiti Mohammed , Sheeba Roshin Raj
Published: February 2019

Modeling of the spectral line and continuum radiation emitted by hydrogen isotopes in peripheral regions of magnetic fusion is presented through profiles of the Zeeman-Doppler broadened Hα/Dα line and those of the Stark broadened high-n Balmer lines extending beyond the series limit for recombining plasmas. The Hα/Dα line profiles should be modelled while accounting for several populations of neutrals to mimic real situations and analyze experimental data for isotopic ratio determination. On the other side, high-n Balmer lines of hydrogen are used for plasma electron density and temperature diagnostics. Moreover, modelling whole spectra including the continuum radiation contributes to the development of synthetic diagnostics for future magnetic fusion devices for which they can give predictive results through coupling to numerical simulation tools.
The Recent Advances in Raman Microscopy and Imaging Techniques for Biosensors
Authors: Rzhevskii Alexander
Published: February 2019

Raman microspectroscopy is now well established as one of the most powerful analytical techniques for a diverse range of applications in physical (material) and biological sciences. Consequently, the technique provides exceptional analytical opportunities to the science and technology of biosensing due to its capability to analyze both parts of a biosensor system—biologically sensitive components, and a variety of materials and systems used in physicochemical transducers. Recent technological developments in Raman spectral imaging have brought additional possibilities in two- and three-dimensional (2D and 3D) characterization of the biosensor’s constituents and their changes on a submicrometer scale in a label-free, real-time nondestructive method of detection. In this report, the essential components and features of a modern confocal Raman microscope are reviewed using the instance of Thermo Scientific DXRxi Raman imaging microscope, and examples of the potential applications of Raman microscopy and imaging for constituents of biosensors are presented.
Nucleotide Weight Matrices Reveal Ubiquitous Mutational Footprints of AID/APOBEC Deaminases in Human Cancer Genomes
Authors: Rogozin Igor B. , Roche-Lima Abiel , Lada Artem G. , Belinky Frida , Sidorenko Ivan A. , Glazko Galina V. , Babenko Vladimir N. , Cooper David N. , Pavlov Youri I.
Published: February 2019

Cancer genomes accumulate nucleotide sequence variations that number in the tens of thousands per genome. A prominent fraction of these mutations is thought to arise as a consequence of the off-target activity of DNA/RNA editing cytosine deaminases. These enzymes, collectively called activation induced deaminase (AID)/APOBECs, deaminate cytosines located within defined DNA sequence contexts. The resulting changes of the original C:G pair in these contexts (mutational signatures) provide indirect evidence for the participation of specific cytosine deaminases in a given cancer type. The conventional method used for the analysis of mutable motifs is the consensus approach. Here, for the first time, we have adopted the frequently used weight matrix (sequence profile) approach for the analysis of mutagenesis and provide evidence for this method being a more precise descriptor of mutations than the sequence consensus approach. We confirm that while mutational footprints of APOBEC1, APOBEC3A, APOBEC3B, and APOBEC3G are prominent in many cancers, mutable motifs characteristic of the action of the humoral immune response somatic hypermutation enzyme, AID, are the most widespread feature of somatic mutation spectra attributable to deaminases in cancer genomes. Overall, the weight matrix approach reveals that somatic mutations are significantly associated with at least one AID/APOBEC mutable motif in all studied cancers.
Lung Cancer Screening, towards a Multidimensional Approach: Why and How?
Authors: Benzaquen Jonathan , Boutros Jacques , Marquette Charles , Delingette Hervé , Hofman Paul
Published: February 2019

Early-stage treatment improves prognosis of lung cancer and two large randomized controlled trials have shown that early detection with low-dose computed tomography (LDCT) reduces mortality. Despite this, lung cancer screening (LCS) remains challenging. In the context of a global shortage of radiologists, the high rate of false-positive LDCT results in overloading of existing lung cancer clinics and multidisciplinary teams. Thus, to provide patients with earlier access to life-saving surgical interventions, there is an urgent need to improve LDCT-based LCS and especially to reduce the false-positive rate that plagues the current detection technology. In this context, LCS can be improved in three ways: (1) by refining selection criteria (risk factor assessment), (2) by using Computer Aided Diagnosis (CAD) to make it easier to interpret chest CTs, and (3) by using biological blood signatures for early cancer detection, to both spot the optimal target population and help classify lung nodules. These three main ways of improving LCS are discussed in this review.
Role of Exosomes in the Regulation of T-Cell Mediated Immune Responses and in Autoimmune Disease
Authors: Anel Alberto , Gallego-Lleyda Ana , de Miguel Diego , Naval Javier , Martínez-Lostao Luis
Published: February 2019

T-cell mediated immune responses should be regulated to avoid the development of autoimmune or chronic inflammatory diseases. Several mechanisms have been described to regulate this process, namely death of overactivated T cells by cytokine deprivation, suppression by T regulatory cells (Treg), induction of expression of immune checkpoint molecules such as CTLA-4 and PD-1, or activation-induced cell death (AICD). In addition, activated T cells release membrane microvesicles called exosomes during these regulatory processes. In this review, we revise the role of exosome secretion in the different pathways of immune regulation described to date and its importance in the prevention or development of autoimmune disease. The expression of membrane-bound death ligands on the surface of exosomes during AICD or the more recently described transfer of miRNA or even DNA inside T-cell exosomes is a molecular mechanism that will be analyzed.
A Comparison of Three Child OHRQoL Measures
Authors: Foster Page Lyndie , Gilchrist Fiona , Broder Hillary L. , Clark Ellen , Thomson W. Murray
Published: February 2019

Comparing oral health-related quality of life (OHRQoL) measures can facilitate selecting the most appropriate one for a particular research question/setting. Three child OHRQoL measures Child Perceptions Questionnaire (CPQ11–14), the Child Oral Health Impact Profile (COHIP) and the Caries Impacts and Experiences Questionnaire for Children (CARIES-QC) were used with 335 10- to 13-year-old participants in a supervised tooth-brushing programme in New Zealand. The use of global questions enabled their validity to be examined. Assessments were conducted at baseline and after 12 months. All three measures had acceptable internal consistency reliability. There were moderate, positive correlations among their scores, and all showed differences in the impact of dental caries on OHRQoL, with children with the highest caries experience having the highest scale scores. Effect sizes were used to assess meaningful change. The CPQ11–14 and the CARIES-QC showed meaningful change. The COHIP-SF score showed no meaningful change. Among children reporting improved OHRQoL, baseline and follow-up scores differed significantly for the CPQ11–14 and CARIES-QC measures, although not for the COHIP-SF. The three scales were broadly similar in their conceptual basis, reliability and validity, but responsiveness of the COHIP-SF was questionable, and the need to compute two different scores for the CARIES-QC meant that its administrative burden was considerably greater than for the other two measures. Replication and use of alternative approaches to measuring meaningful change are suggested.
Use of Fire-Extinguishing Balls for a Conceptual System of Drone-Assisted Wildfire Fighting
Authors: Aydin Burchan , Selvi Emre , Tao Jian , Starek Michael
Published: February 2019

This paper examines the potential use of fire extinguishing balls as part of a proposed system, where drone and remote-sensing technologies are utilized cooperatively as a supplement to traditional firefighting methods. The proposed system consists of (1) scouting unmanned aircraft system (UAS) to detect spot fires and monitor the risk of wildfire approaching a building, fence, and/or firefighting crew via remote sensing, (2) communication UAS to establish and extend the communication channel between scouting UAS and fire-fighting UAS, and (3) a fire-fighting UAS autonomously traveling to the waypoints to drop fire extinguishing balls (environmental friendly, heat activated suppressants). This concept is under development through a transdisciplinary multi-institutional project. The scope of this paper encloses general illustration of this design, and the experiments conducted so far to evaluate fire extinguishing balls. The results of the experiments show that smaller size fire extinguishing balls available in the global marketplace attached to drones might not be effective in aiding in building fires (unless there are open windows in the buildings already). On the contrary, results show that even the smaller size fire extinguishing balls might be effective in extinguishing short grass fires (around 0.5 kg size ball extinguished a circle of 1-meter of short grass). This finding guided the authors towards wildfire fighting rather than building fires. The paper also demonstrates building of heavy payload drones (around 15 kg payload), and the progress of development of an apparatus carrying fire-extinguishing balls attachable to drones.
The Optimized Multi-Scale Permutation Entropy and Its Application in Compound Fault Diagnosis of Rotating Machinery
Authors: Wang Xianzhi , Si Shubin , Wei Yu , Li Yongbo
Published: February 2019

Multi-scale permutation entropy (MPE) is a statistic indicator to detect nonlinear dynamic changes in time series, which has merits of high calculation efficiency, good robust ability, and independence from prior knowledge, etc. However, the performance of MPE is dependent on the parameter selection of embedding dimension and time delay. To complete the automatic parameter selection of MPE, a novel parameter optimization strategy of MPE is proposed, namely optimized multi-scale permutation entropy (OMPE). In the OMPE method, an improved Cao method is proposed to adaptively select the embedding dimension. Meanwhile, the time delay is determined based on mutual information. To verify the effectiveness of OMPE method, a simulated signal and two experimental signals are used for validation. Results demonstrate that the proposed OMPE method has a better feature extraction ability comparing with existing MPE methods.
Experimental Study on the Physical Performance and Flow Behavior of Decorated Polyacrylamide for Enhanced Oil Recovery
Authors: Liang Shuang , Liu Yikun , Hu Shaoquan , Shen Anqi , Yu Qiannan , Yan Hua , Bai Mingxing
Published: February 2019

With the rapid growth of energy consumption, enhanced oil recovery (EOR) methods are continually emerging, the most effective and widely used was polymer flooding. However, the shortcomings were gradually exposed. A novel decorated polyacrylamide might be a better alternative than polymer. In this work, the molecular structure and the properties reflecting the viscosity of decorated polyacrylamide, interfacial tension, and emulsification were examined. In order to better understand the interactions between decorated polyacrylamide and oil as well as the displacement mechanism, the displacement experiment were conducted in the etched-glass microscale model. Moreover, the coreflooding comparison experiments between decorated polyacrylamide and polymer were performed to investigate the displacement effect. The statistical analysis showed that the decorated polyacrylamide has excellent characteristics of salt tolerance, viscosity stability, and viscosification like polymer. Besides, the ability to reduce the interfacial tension in order 10−1 and emulsification, which were more similar to surfactant. Therefore, the decorated polyacrylamide was a multifunctional polymer. The displacement process captured by camera illustrated that the decorated polyacrylamide flooded oil mainly by means of ‘pull and drag’, ‘entrainment’, and ‘bridging’, based on the mechanism of viscosifying, emulsifying, and viscoelasticity. The results of the coreflooding experiment indicated that the recovery of decorated polyacrylamide can be improved by approximately 11–16% after water flooding when the concentration was more than 800 mg/L, which was higher than that of conventional polymer flooding. It should be mentioned that a new injection mode of ‘concentration reduction multi-slug’ was first proposed, and it obtained an exciting result of increasing oil production and decreasing water-cut, the effect of conformance control was more significant.
Modeling a High Concentrator Photovoltaic Module Using Fuzzy Rule-Based Systems
Authors: Gadeo-Martos Manuel Angel , Yuste-Delgado Antonio Jesús , Almonacid Cruz Florencia , Fernandez-Prieto Jose-Angel , Canada-Bago Joaquin
Published: February 2019

Currently, there is growing interest in the modeling of high concentrator photovoltaic modules, due to the importance of achieving an accurate model, to improve the knowledge and understanding of this technology and to promote its expansion. In recent years, some techniques of artificial intelligence, such as the Artificial Neural Network, have been used with the goal of obtaining an electrical model of these modules. However, little attention has been paid to applying Fuzzy Rule-Based Systems for this purpose. This work presents two new models of high concentrator photovoltaics that use two types of Fuzzy Systems: the Takagi-Sugeno-Kang, characterized by the achievement of high accuracy in the model, and the Mamdani, characterized by high accuracy and the ease of interpreting the linguistic rules that control the behavior of the fuzzy system. To obtain a good knowledge base, two learning methods have been proposed: the “Adaptive neuro-fuzzy inference system” and the “Ad Hoc data-driven generation”. These combinations of fuzzy systems and learning methods have allowed us to obtain two models of high concentrator photovoltaic modules, which include two improvements over previous models: an increase in the model accuracy and the possibility of deducing the relationship between the main meteorological parameters and the maximum power output of a module.
Influence of Disc Tip Geometry on the Aerodynamic Performance and Flow Characteristics of Multichannel Tesla Turbines
Authors: Qi Wenjiao , Deng Qinghua , Chi Zhinan , Hu Lehao , Yuan Qi , Feng Zhenping
Published: February 2019

As a competitive small-scale turbomachinery option, Tesla turbines have wide potential in various fields, such as renewable energy generation systems and small power equipment. This paper investigates the influence of disc tip geometry, including its profile and relative height, on the aerodynamic performance and flow characteristics of one-to-one and one-to-many multichannel Tesla turbines. The results indicate that compared to the turbine with blunt tips, the isentropic efficiency of the one-to-one turbine with sharp tips has a little decrease, which is because the relative tangential velocity gradient near the rotational disc walls decreases a little and additional vortices are generated at the rotor inlet, while that of the one-to-many turbine with sharp tips increases significantly, resulting from an increase in the relative tangential velocity in the disc channels and a decrease in the low Mach number and vortex area; for instance the turbine efficiency for the former relatively decreases by 3.6% and that for the latter increases by 13.5% at 30,000 r/min. In addition, the isentropic efficiency of the one-to-many turbine with sharp tips goes up with increasing relative height due to increasing improvement of flow status, and its increment rate slows down. A circular or elliptic tip performs better with lower relative height and a triangular tip behaves better with higher relative height. To sum up, a blunt disc tip is recommended for the one-to-one turbine, and a sharp disc tip is for the one-to-many turbine. The relative height and tip profile of the one-to-many turbine should be determined according to their effects on turbine performance, manufacturing difficulty and mechanical deformation.
Methodology for the Energy Need Assessment to Effectively Design and Deploy Mini-Grids for Rural Electrification
Authors: Gambino Valeria , Del Citto Riccardo , Cherubini Paolo , Tacconelli Carlo , Micangeli Andrea , Giglioli Romano
Published: February 2019

In order to successfully deploy a large number of decentralized energy systems in developing countries, it is necessary to standardize effective methodologies and procedures to develop off-grid/mini-grid systems. Considering that the energy need assessment provides inputs and assumptions used in business modelling and mini-grid design, the accuracy of its results directly affects the technical and financial feasibility studies. Thus, the approach for applying a proven methodology for the energy need assessment of rural communities is aimed at obtaining reliable input data for the mini-grid development. This helps in reducing both the financial challenges by mitigating the uncertainties in electricity demand and the technical challenges by contributing to adequately size off-grid power generation systems, with a view to boost toward a common overall objective of mini-grid’s optimization methods and tools. Hence, taking into consideration that target communities differ in terms of needs and context conditions, the proposed paper describes an inclusive methodology that can be adapted case-by-case. It provides an effective applied solution the lack of proven guidelines from project developers or literature, giving priority to data collection methods able to achieve a large sample representative of the market, with high accuracy in estimating the energy consumptions from electricity substitutes.
Effects of Experimental Nitrogen Addition on Nutrients and Nonstructural Carbohydrates of Dominant Understory Plants in a Chinese Fir Plantation
Authors: Wang Fangchao , Chen Fusheng , Wang G. Geoff , Mao Rong , Fang Xiangmin , Wang Huimin , Bu Wensheng
Published: February 2019

Research Highlights: This study identifies the nitrogen (N) deposition effect on understory plants by altering directly soil nutrients or indirectly altering environmental factors in subtropical plantation. Background and Objectives: N deposition is a major environmental issue and has altered forest ecosystem components and their functions. The response of understory vegetation to N deposition is often neglected due to a small proportion of stand productivity. However, compared to overstory trees, understory species usually have a higher nutrient cycle rate and are more sensitive to environmental change, so should be of greater concern. Materials and Methods: The changes in plant biomass, N, phosphorus (P), and nonstructural carbohydrates (NSCs) of three dominant understory species, namely Dicranopteris dichotoma, Lophatherum gracile, and Melastoma dodecandrum, were determined following four years of experimental N addition (100 kg hm−2 year−1 of N) in a Chinese fir plantation. Results: N addition increased the tissue N concentrations of all the understory plants by increasing soil mineral N, while N addition decreased the aboveground biomass of D. dichotoma and L. gracile significantly—by 82.1% and 67.2%, respectively. The biomass of M. dodecandrum did not respond to N addition. In contrast, N addition significantly increased the average girth growth rates and litterfall productivity of overstory trees—by 18.28% and 36.71%, respectively. NSCs, especially soluble sugar, representing immediate products of photosynthesis and main energy sources for plant growth, decreased after N addition in two of the three species. The plant NSC/N and NSC/P ratios showed decreasing tendencies, but the N/P ratio in aboveground tissue did not change with N addition. Conclusions: N addition might inhibit the growth of understory plants by decreasing the nonstructural carbohydrates and light availability indirectly rather than by changing nutrients and N/P stoichiometry directly, although species-specific responses to N deposition occurred in the Chinese fir plantation.
Initial Location Preference Together with Aggregation Pheromones Regulate the Attack Pattern of Tomicus brevipilosus (Coleoptera: Curculionidae) on Pinus kesiya
Authors: Liu Fu , Wu Chengxu , Zhang Sufang , Kong Xiangbo , Zhang Zhen , Wang Pingyan
Published: February 2019

Research Highlights: We found that the initial attack location together with the aggregation pheromones played an important role in mediating the aggressive behavior of T. brevipilosus on P. kesiya. Background and Objectives: T. brevipilosus was identified as an aggressive species, which possesses the ability to kill live, healthy P. kesiya. In this scenario, we study the top-down attack pattern of T. brevipilosus on P. kesiya during the entirety of the reproductive period. Materials and Methods: We investigated the phenology of trunk attack on P. kesiya over a period of three years in Pu’er City, China. The hindguts extracts of the females and males T. brevipilosus were analyzed by coupled gas chromatography-mass spectrometry (GC-MS). The candidate aggregation pheromone compounds of T. brevipilosus were determined through electrophysiology experiments (electroantennographic detection, EAD and electroantennography, EAG), laboratory olfactometer bioassays, and field trapping. Results: we found that the pioneer beetles preferentially infested the crown of P. kesiya at the early stage of attack following spring flight with the later arriving beetles selectively attacking the lower area of the trunk to avoid intraspecific competition and better utilize limited resources, which exhibits a top-down attack pattern. During gallery initiation, the beetles release aggregation pheromones to attract conspecifics to conduct a mass attack. The chemical analyses indicated that the hindgut extracts of gallery-initiating beetles contained a larger amount of myrtenol, cis-verbenol, trans-verbenol, and verbenone. Myrtenol and trans-verbenol were identified as candidate aggregation pheromone compounds. In addition, a blend of these two components with S-(−)-α-pinene and S-(−)-β-pinene attracted more T. brevipilosus individuals in a field bioassay. Conclusions: We concluded that the preference for the initial attack location together with the aggregation pheromones played an important role in mediating the top-down attack pattern of T. brevipilosus on P. kesiya.
Sensory Profile and Acceptability of HydroSOStainable Almonds
Authors: Lipan Leontina , Cano-Lamadrid Marina , Corell Mireia , Sendra Esther , Hernández Francisca , Stan Laura , Vodnar Dan Cristian , Vázquez-Araújo Laura , Carbonell-Barrachina Ángel A.
Published: February 2019

Fresh water availability is considered highly risky because it is a finite resource, and a deficiency in water leads to numerous economic and environmental issues. Agriculture is one of the main consumers of fresh water in practices such as irrigation and fertilization. In this context, the main objectives of this study were (i) to determine the descriptive sensory profiles of four almond types grown using different irrigation strategies and (ii) to study their acceptance in a cross-cultural study (Romania and Spain). Consumers’ willingness to pay for hydroSOS almonds was also evaluated. The four irrigation strategies evaluated were a control sample, two samples grown under regulated deficit irrigation strategies (RDI), and a sample grown under a sustained deficit irrigation strategy (SDI). The main conclusion was that neither descriptive nor affective sensory results showed significant differences among treatments. These findings should encourage farmers to reduce their water usage by demonstrating that sensory quality was not significantly affected by any of the studied treatments, compared to the control. Regarding willingness to pay, both Spanish and Romanian consumers were willing to pay a higher price for the hydroSOS almonds.
Influence of Carob Flour and Carob Bean Gum on Rheological Properties of Cocoa and Carob Pastry Fillings
Authors: Benković Maja , Bosiljkov Tomislav , Semić Amela , Ježek Damir , Srečec Siniša
Published: February 2019

The aim of this study was to develop a new cocoa and carob based pastry filling and explore the influences of carob flour and carob gum on the rheological and textural properties, specifically (i) the effect of increasing the amount of carob flour and (ii) the effect of carob bean gum naturally present in the carob flour with seeds versus the commercially available carob bean gum. All samples analyzed in this study exhibited shear thinning behavior. The texture analysis revealed a significant (p < 0.01) increase in consistency and firmness in samples with higher amounts of carob flour added, while higher temperatures significantly (p < 0.01) decreased adhesiveness. When comparing naturally occurring and commercially available LBG (locust bean gum), it was concluded that lower concentrations (up to 0.45% w/w) of naturally occurring LBG work just as well at the same concentrations of commercially available LBG, but this effect cannot be confirmed for higher LBG concentrations, nor for rheological properties determined at higher temperatures (80 °C).
Lysozyme Aptamer-Functionalized Magnetic Nanoparticles for the Purification of Lysozyme from Chicken Egg White
Authors: Luo Ruiping , Zhou Xinrui , Chen Yan , Tuo Sicheng , Jiang Fulin , Niu Xiaodi , Pan Fengguang , Wang Hongsu
Published: February 2019

Lysozyme is in high demand due to its many favorable characteristics such as being naturally occurring, non-toxic, and easy to digest and absorb. Recently, superparamagnetic nanoparticles with strong magnetic responsiveness have attracted significant interest for enzyme purification. The aptamer of the enzyme can be chemically synthesized rapidly at a large scale using simple and low-cost preparation methods. Therefore, Fe3O4 nanoparticles (Fe3O4 NPs) were prepared by chemical co-precipitation and were then functionalized with amino groups to produce NH2-Fe3O4 NPs. The specific reaction of aldehyde and amino groups was used to attach lysozyme aptamers with specific sequences to NH2-Fe3O4 NPs to produce Apt-NH2-Fe3O4 NPs. The synthesized materials were characterized using transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), hysteresis loop analysis, and thermogravimetric analysis (TGA). The optimal experimental conditions for adsorption of lysozyme were investigated. The effects of initial lysozyme concentration, adsorption time, pH, reaction temperature, and ionic strength were determined. The maximum adsorption capacity and relevant activity of Apt-NH2-Fe3O4 NPs was 460 mg·g−1 and 16,412 ± 55 U·mg−1 in an aqueous lysozyme solution. In addition, as demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis analysis, lysozyme could be separated from crude fresh egg white using Apt-NH2-Fe3O4 NPs with an amount up to 113 ± 4.2 mg·g−1 and an activity up to 16,370 ± 46 U·mg−1.
KDM4B: A Nail for Every Hammer?
Authors: Wilson Cailin , Krieg Adam J.
Published: February 2019

Epigenetic changes are well-established contributors to cancer progression and normal developmental processes. The reversible modification of histones plays a central role in regulating the nuclear processes of gene transcription, DNA replication, and DNA repair. The KDM4 family of Jumonj domain histone demethylases specifically target di- and tri-methylated lysine 9 on histone H3 (H3K9me3), removing a modification central to defining heterochromatin and gene repression. KDM4 enzymes are generally over-expressed in cancers, making them compelling targets for study and therapeutic inhibition. One of these family members, KDM4B, is especially interesting due to its regulation by multiple cellular stimuli, including DNA damage, steroid hormones, and hypoxia. In this review, we discuss what is known about the regulation of KDM4B in response to the cellular environment, and how this context-dependent expression may be translated into specific biological consequences in cancer and reproductive biology.
Special Issue: Fungal Pathogenesis in Humans: The Growing Threat
Authors: Leal Fernando
Published: February 2019

Approximately 150 fungal species are considered as primary pathogens of humans and animals [...]
Parental Psychological Control and Emotional and Behavioral Disorders among Spanish Adolescents
Authors: León-del-Barco Benito , Mendo-Lázaro Santiago , Polo-del-Río María I. , López-Ramos Víctor M.
Published: February 2019

There is no denying the fundamental role played by parents in the psychosocial development of their children—either as a liability or as protection against mental health disorders. This study seeks to ascertain, by means of odds ratio statistics (OR), the correlation between parental psychological control and emotional and behavioral disorders. A total of 762 students took part in this study, with an average age of 12.23 years—53.8% of whom were girls and 46.2% were boys. Children and adolescents reported their parental psychological control and their emotional and behavioral disorders (i.e., emotional and behavioral problems, internalizing and externalizing problems). Minors who perceive their psychological control as high are 6 times more likely to suffer from internalizing disorders and 4.8 times more likely to develop externalizing disorders. Furthermore, the probability of suffering externalizing disorders is higher among males who perceive a high degree of psychological control. This study breaks new ground on the importance of perceived psychological control—considered as a negative form of control by parents—in the emotional and behavioral disorders among children and adolescents.
Promoting Healthcare Sustainability in Developing Countries: Analysis of Knowledge Management Drivers in Public and Private Hospitals of Pakistan
Authors: Karamat Jawad , Shurong Tong , Ahmad Naveed , Afridi Sana , Khan Shahbaz , Mahmood Kashif
Published: February 2019

Investing in a sustainable future has no alternative; the healthcare sector in developing countries has failed to achieve sustainability objectives. Knowledge management (KM) is a concrete application of sustainability in healthcare, as organizations (hospitals) that manage their knowledge assets will gain sustainable competitive advantage. Several organizations in developed countries are moving towards the adoption of knowledge management so that they can manage their knowledge well and improve their performance. Due to the effective implementation of KM in developed countries, developing countries are also considering adopting KM in their healthcare. In this study, an attempt has been made to identify the drivers of KM adoption in public and private hospitals of Pakistan. With the help of an extensive literature review and expert opinion, the drivers were identified and a hierarchical structure was developed. Nineteen drivers were identified and screened out by experts. The experts identified the contextual relationships between the drivers during a brainstorming session. The hierarchical model of the drivers for KM in the healthcare of Pakistan was eventually developed using interpretive structural modeling (ISM). The structure has 10 levels, in which “developed competitive advantage” formed the foundation of the structure and “job creation” and “improvement in the reputation of healthcare” formed the topmost level. The “Matrices d’Impacts Croises Multiplication Appliqué a un Classement” (MICMAC) analysis classified the drivers by categorizing them according to their driving and dependence powers. One driver is identified as autonomous, six drivers as dependent, seven drivers as linkage, and five drivers as independent. The analysis of KM drivers will provide a good understanding of the interdependence and interactions between them and support the effect adoption of KM in developing countries especially in Pakistan.
Quantitative Evaluation of the Eco-Environment in a Coalfield Based on Multi-Temporal Remote Sensing Imagery: A Case Study of Yuxian, China
Authors: Wang Xue , Tan Kun , Xu Kailei , Chen Yu , Ding Jianwei
Published: February 2019

With the exploitation of coalfields, the eco-environment around the coalfields can become badly damaged. To address this issue, “mine greening” has been proposed by the Ministry of Land and Resources of China. The sustainable development of mine environments has now become one of the most prominent issues in China. In this study, we aimed to make use of Landsat 7 ETM+ and Landsat 8 OLI images obtained between 2005 and 2016 to analyze the eco-environment in a coalfield. Land cover was implemented as the basic evaluation factor to establish the evaluation model for the eco-environment. Analysis and investigation of the eco-environment in the Yuxian coalfield was conducted using a novel evaluation model, based on the biological abundance index, vegetation coverage index, water density index, and natural geographical factors. The weight of each indicator was determined by an analytic hierarchy process. Meanwhile, we also used the classic ecological footprint to calculate the ecological carrying capacity in order to verify the effectiveness of the evaluation model. Results showed that the eco-environment index illustrated a slowly increasing tendency over the study period, and the ecological quality could be considered as “good”. The results of the evaluation model showed a strong correlation with the ecological carrying capacity with a correlation coefficient of 0.9734. In conclusion, the evaluation method is a supplement to the time-series quantitative evaluation of the eco-environment, and also helps us to explore the eco-environment in the mining area.
Development and Validation of the Vending Evaluation for Nutrient-Density (VEND)ing Audit
Authors: Horacek Tanya M. , Yildirim Elif Dede , Matthews Schreiber Melissa , Byrd-Bredbenner Carol , Colby Sarah , White Adrienne A. , Shelnutt Karla P. , Olfert Melissa D. , Mathews Anne E. , Riggsbee Kristin , Franzen-Castle Lisa , Morrell Jesse Stabile , Kattelmann Kendra
Published: February 2019

Background: This paper describes the development and validation of the Vending Evaluation for Nutrient-Density (VEND)ing audit to comprehensively evaluate vended products based upon healthfulness, price and promotion, and machine accessibility. Methods: A novel vending nutrient-density score was created to determine the healthfulness of vended snack/beverage products. Field tested in United States colleges, VENDing audit (∑nutrient-density + 10 × % healthy products) and Support sub-scores (price + promotion + accessibility) were calculated for snack/beverage machines. Higher scores indicate more healthful vending options and supports for choosing healthfully. Nutrition Environment Measures Survey-Vending (NEMS-V) was used to validate the nutrient-density score for a sub-sample of machines. Sensitivity and specificity were computed by comparing the number of healthy snacks/beverages determined by NEMS-V and the VENDing nutrient-density scores. Results: Researchers conducted the VENDing audit on 228 snack/beverage vending machines at 9 universities within the United States and used both VENDing and NEMS-V on 33 snack and 52 beverage vending machines. Mean VENDing audit scores were 4.5 ± 2.0 (2.6, 3.4) and 2.6 ± 2.0 (0, 12) for snack/beverage machines, respectively. The number of products considered healthy assessed with both the VENDing nutrient-density scores and the NEMS-V were positively correlated for beverages (r = 0.687, p < 0.001) and snacks (r = 0.366, p < 0.05). The sensitivity was excellent for beverages (0.83) and moderate for snacks (0.69); while the specificity was moderate for both beverages (0.66) and snacks (0.50). Conclusions: The VENDing audit uses unique, valid, and reliable nutrient-density scoring to evaluate snacks/beverages along a continuum of healthful criteria and comprehensively evaluates the full vending environment.
MIST1, an Inductive Signal for Salivary Amylase in Mesenchymal Stem Cells
Authors: Mona Mahmoud , Miller Rehae , Li Hui , Park Yun-Jong , Zaman Raafi , Yang Li-Jun , Cha Seunghee
Published: February 2019

Sjögren’s syndrome (SjS) is an autoimmune disease that destroys the salivary glands and results in severe dry mouth. Mesenchymal stem cell (MSC) transplantation has been recently proposed as a promising therapy for restoring cells in multiple degenerative diseases. We have recently utilized advanced proteomics biochemical assays to identify the key molecules involved in the mesenchymal-epithelial transition (MET) of co-cultured mouse bone-marrow-derived MSCs mMSCs with primary salivary gland cells. Among the multiple transcription factors (TFs) that were differentially expressed, two major TFs were selected: muscle, intestine, and stomach expression-1 (MIST1) and transcription factor E2a (TCF3). These factors were assessed in the current study for their ability to drive the expression of acinar cell marker, alpha-salivary amylase 1 (AMY1), and ductal cell marker, cytokeratin19 (CK19), in vitro. Overexpression of MIST1-induced AMY1 expression while it had little effect on CK19 expression. In contrast, TCF3 induced neither of those cellular markers. Furthermore, we have identified that mMSCs express muscarinic-type 3 receptor (M3R) mainly in the cytoplasm and aquaporin 5 (AQP5) in the nucleus. While MIST1 did not alter M3R levels in mMSCs, a TCF3 overexpression downregulated M3R expressions in mMSCs. The mechanisms for such differential regulation of glandular markers by these TFs warrant further investigation.
The Importance of Conserved Serine for C-Terminally Encoded Peptides Function Exertion in Apple
Authors: Yu Zipeng , Xu Yang , Liu Lin , Guo Yarong , Yuan Xisen , Man Xinyu , Liu Chang , Yang Guodong , Huang Jinguang , Yan Kang , Zheng Chengchao , Wu Changai , Zhang Shizhong
Published: February 2019

Background: The C-terminally encoded peptide (CEP) family has been shown to play vital roles in plant growth. Although a genome-wide analysis of this family has been performed in Arabidopsis, little is known regarding CEPs in apple (Malus domestica). Methods: Here, a comprehensive bioinformatics approach was applied to identify MdCEPs in apple, and 12 MdCEP genes were identified and distributed on 6 chromosomes. Results: MdCEP1 peptide had an inhibitory effect on root growth of apple seedlings, indicating that MdCEP1 played a negative role in root development. In addition, the serine and glycine residues remained conserved within the CEP domains, and MdCEP1 lost its function after mutation of these two key amino acids, suggesting that Ser10 and Gly14 residues are crucial for MdCEPs-mediated root growth of apple. Encouragingly, multiple sequence alignment of 273 CEP domains showed that Ser10 residue was evolutionarily conserved in monocot and eudicot plants. MdCEP derivative (Ser to Cys) lost the ability to inhibit the root growth of Nicotiana benthamiana, Setaria italic, Samolous parviflorus, and Raphanus sativus L. and up-regulate the NO3− importer gene NRT2.1. Conclusion: Taken together, Ser10 residue is crucial for CEP function exertion in higher land plants, at least in apple.
Exogenous Application of Phytohormones Promotes Growth and Regulates Expression of Wood Formation-Related Genes in Populus simonii × P. nigra
Authors: Yuan Hongmei , Zhao Lijuan , Guo Wendong , Yu Ying , Tao Lei , Zhang Liguo , Song Xixia , Huang Wengong , Cheng Lili , Chen Jing , Guan Fengzhi , Wu Guangwen , Li Huiyu
Published: February 2019

Although phytohormones are known to be important signal molecules involved in wood formation, their roles are still largely unclear. Here, Populus simonii × P. nigra seedlings were treated with different concentrations of exogenous phytohormones, indole-3-acetic acid (IAA), gibberellin (GA3), and brassinosteroid (BR), and the effects of phytohormones on growth were investigated. Next, 27 genes with known roles in wood formation were selected for qPCR analysis to determine tissue-specificity and timing of responses to phytohormone treatments. Compared to the control, most IAA, GA3, and BR concentrations significantly increased seedling height. Meanwhile, IAA induced significant seedling stem diameter and cellulose content increases that peaked at 3 and 30 mg·L−1, respectively. Significant increase in cellulose content was also observed in seedlings treated with 100 mg·L−1 GA3. Neither stem diameter nor cellulose content of seedlings were affected by BR treatment significantly, although slight effects were observed. Anatomical measurements demonstrated improved xylem, but not phloem, development in IAA- and BR-treated seedlings. Most gene expression patterns induced by IAA, GA3, and BR differed among tissues. Many IAA response genes were also regulated by GA3, while BR-induced transcription was weaker and slower in Populus than for IAA and GA3. These results reveal the roles played by phytohormones in plant growth and lay the foundation for exploring molecular regulatory mechanisms of wood formation in Populus.
Analyzing Patterns in NewSTEPs Site Review Recommendations: Practical Applications for Newborn Screening Programs
Authors: Kellar-Guenther Yvonne , Sontag Marci K. , Linder Eric , Singh Sikha , Sheller Ruthanne , Ojodu Jelili
Published: February 2019

The Newborn Screening Technical assistance and Evaluation Program (NewSTEPs) conducts non-regulatory site reviews of state newborn screening programs in the US with the goal of providing comprehensive reports and recommendations to support quality improvements within the system. A detailed coding and qualitative analysis of data extracted from reports of seven programs visited between 2012 and 2017, of thirteen pre-site visit surveys completed by state newborn screening programs, and of information from interviews conducted with three site review experts revealed four common themes that exist across states within the national newborn screening system. These themes include opportunities to implement improvements in: (1) communications inside and outside of the state newborn screening program, (2) education, (3) information technology, and (4) operations. The cross-cutting recommendations provided by NewSTEPs within the comprehensive site review reports may prove valuable for all state programs to consider and to incorporate as quality improvement measures in the absence of a full site review. The analysis of the site review process and recommendations identified important opportunities for improvement, many of which were previously unknown to be common across programs, and also provided affirmation of known challenges.