bluuebunny/crossref_metadata_2025 huggingface dataset:
Emerging Roles of TRIM8 in Health and Disease
Authors: Marzano Flaviana , Guerrini Luisa , Pesole Graziano , Sbisà Elisabetta , Tullo Apollonia
Published: March 2021

The superfamily of TRIM (TRIpartite Motif-containing) proteins is one of the largest groups of E3 ubiquitin ligases. Among them, interest in TRIM8 has greatly increased in recent years. In this review, we analyze the regulation of TRIM8 gene expression and how it is involved in many cell reactions in response to different stimuli such as genotoxic stress and attacks by viruses or bacteria, playing a central role in the immune response and orchestrating various fundamental biological processes such as cell survival, carcinogenesis, autophagy, apoptosis, differentiation and inflammation. Moreover, we show how TRIM8 functions are not limited to ubiquitination, and contrasting data highlight its role either as an oncogene or as a tumor suppressor gene, acting as a “double-edged weapon”. This is linked to its involvement in the selective regulation of three pivotal cellular signaling pathways: the p53 tumor suppressor, NF-κB and JAK-STAT pathways. Lastly, we describe how TRIM8 dysfunctions are linked to inflammatory processes, autoimmune disorders, rare developmental and cardiovascular diseases, ischemia, intellectual disability and cancer.
Neutrophil Adhesion and the Release of the Free Amino Acid Hydroxylysine
Authors: Galkina Svetlana , Fedorova Natalia , Ksenofontov Alexander , Serebryakova Marina , Golenkina Ekaterina , Stadnichuk Vladimir , Baratova Ludmila , Sud’ina Galina
Published: March 2021

During infection or certain metabolic disorders, neutrophils can escape from blood vessels, invade and attach to other tissues. The invasion and adhesion of neutrophils is accompanied and maintained by their own secretion. We have previously found that adhesion of neutrophils to fibronectin dramatically and selectively stimulates the release of the free amino acid hydroxylysine. The role of hydroxylysine and lysyl hydroxylase in neutrophil adhesion has not been studied, nor have the processes that control them. Using amino acid analysis, mass spectrometry and electron microscopy, we found that the lysyl hydroxylase inhibitor minoxidil, the matrix metalloproteinase inhibitor doxycycline, the PI3K/Akt pathway inhibitors wortmannin and the Akt1/2 inhibitor and drugs that affect the actin cytoskeleton significantly and selectively block the release of hydroxylysine and partially or completely suppress spreading of neutrophils. The actin cytoskeleton effectors and the Akt 1/2 inhibitor also increase the phenylalanine release. We hypothesize that hydroxylysine release upon adhesion is the result of the activation of lysyl hydroxylase in interaction with matrix metalloproteinase, the PI3K/Akt pathway and intact actin cytoskeleton, which play important roles in the recruitment of neutrophils into tissue through extracellular matrix remodeling.
Mitochondrial Dysfunction and Permeability Transition in Neonatal Brain and Lung Injuries
Authors: S. Ten Vadim , Stepanova Anna A. , Ratner Veniamin , Neginskaya Maria , Niatsetskaya Zoya , Sosunov Sergey , Starkov Anatoly
Published: March 2021

This review discusses the potential mechanistic role of abnormally elevated mitochondrial proton leak and mitochondrial bioenergetic dysfunction in the pathogenesis of neonatal brain and lung injuries associated with premature birth. Providing supporting evidence, we hypothesized that mitochondrial dysfunction contributes to postnatal alveolar developmental arrest in bronchopulmonary dysplasia (BPD) and cerebral myelination failure in diffuse white matter injury (WMI). This review also analyzes data on mitochondrial dysfunction triggered by activation of mitochondrial permeability transition pore(s) (mPTP) during the evolution of perinatal hypoxic-ischemic encephalopathy. While the still cryptic molecular identity of mPTP continues to be a subject for extensive basic science research efforts, the translational significance of mitochondrial proton leak received less scientific attention, especially in diseases of the developing organs. This review is focused on the potential mechanistic relevance of mPTP and mitochondrial dysfunction to neonatal diseases driven by developmental failure of organ maturation or by acute ischemia-reperfusion insult during development.
The Legacy of Parker, Baker and Smith 1972: Gamete Competition, the Evolution of Anisogamy, and Model Robustness
Authors: Lehtonen Jussi
Published: March 2021

The evolution of anisogamy or gamete size dimorphism is a fundamental transition in evolutionary history, and it is the origin of the female and male sexes. Although mathematical models attempting to explain this transition have been published as early as 1932, the 1972 model of Parker, Baker, and Smith is considered to be the first explanation for the evolution of anisogamy that is consistent with modern evolutionary theory. The central idea of the model is ingenious in its simplicity: selection simultaneously favours large gametes for zygote provisioning, and small gametes for numerical competition, and under certain conditions the outcome is anisogamy. In this article, I derive novel analytical solutions to a 2002 game theoretical update of the 1972 anisogamy model, and use these solutions to examine its robustness to variation in its central assumptions. Combining new results with those from earlier papers, I find that the model is quite robust to variation in its central components. This kind of robustness is crucially important in a model for an early evolutionary transition where we may only have an approximate understanding of constraints that the different parts of the model must obey.
High Density Lipoprotein Cholesterol Efflux Capacity and Atherosclerosis in Cardiovascular Disease: Pathophysiological Aspects and Pharmacological Perspectives
Authors: Adorni Maria Pia , Ronda Nicoletta , Bernini Franco , Zimetti Francesca
Published: March 2021

Over the years, the relationship between high-density lipoprotein (HDL) and atherosclerosis, initially highlighted by the Framingham study, has been revealed to be extremely complex, due to the multiple HDL functions involved in atheroprotection. Among them, HDL cholesterol efflux capacity (CEC), the ability of HDL to promote cell cholesterol efflux from cells, has emerged as a better predictor of cardiovascular (CV) risk compared to merely plasma HDL-cholesterol (HDL-C) levels. HDL CEC is impaired in many genetic and pathological conditions associated to high CV risk such as dyslipidemia, chronic kidney disease, diabetes, inflammatory and autoimmune diseases, endocrine disorders, etc. The present review describes the current knowledge on HDL CEC modifications in these conditions, focusing on the most recent human studies and on genetic and pathophysiologic aspects. In addition, the most relevant strategies possibly modulating HDL CEC, including lifestyle modifications, as well as nutraceutical and pharmacological interventions, will be discussed. The objective of this review is to help understanding whether, from the current evidence, HDL CEC may be considered as a valid biomarker of CV risk and a potential pharmacological target for novel therapeutic approaches.
Correlation of Intravascular Ultrasound with Histology in Pediatric Pulmonary Vein Stenosis
Authors: Callahan Ryan , Gauthier Zachary , Toba Shuhei , Sanders Stephen P. , Porras Diego , Vargas Sara O.
Published: March 2021

Preliminary intravascular ultrasound (IVUS) images of suspected pediatric intraluminal pulmonary vein stenosis (PVS) demonstrate wall thickening. It is unclear how the IVUS-delineated constituents of wall thickening correlate with the histology. We analyzed six postmortem formalin-fixed heart/lung specimens and four live patients with PVS as well as control pulmonary veins using IVUS and light microscopic examination. In PVS veins, IVUS demonstrated wall thickening with up to two layers of variable echogenicity, often with indistinct borders. Histologically, the veins showed fibroblastic proliferation with areas rich in myxoid matrix as well as areas with abundant collagen and elastic fibers. Discrete vein layers were obscured by scarring and elastic degeneration. A lower reflective periluminal layer by IVUS corresponded with hyperplasia of myofibroblast-like cells in abundant myxoid matrix. The hyper-reflective layer by IVUS extended to the outer edge of the vessel and corresponded to a less myxoid area with more collagen, smooth muscle and elastic fibers. The outer less reflective edge of the IVUS image correlated with a gradual transition into adventitia. Normal veins had a thin wall, correlating with histologically normal cellular and extracellular components, without intimal proliferation. IVUS may provide further understanding of the anatomy and mechanisms of pediatric pulmonary vein obstruction.
Developments in Chemical Treatments, Manufacturing Techniques and Potential Applications of Natural-Fibers-Based Biodegradable Composites
Authors: Khalid Muhammad Yasir , Imran Ramsha , Arif Zia Ullah , Akram Naveed , Arshad Hassan , Al Rashid Ans , García Márquez Fausto Pedro
Published: March 2021

The utilization of synthetic materials stimulates environmental concerns, and researchers worldwide are effectively reacting to environmental concerns by transitioning towards biodegradable and sustainable materials. Natural fibers like jute and sisal have been being utilized for ages in several applications, such as ropes, building materials, particle boards, etc. The absence of essential information in preparing the natural-fiber-reinforced materials is still a challenge for future applications. Chemical treatments and surface modifications can improve the quality of the natural fibers. Natural-fiber-based composites are a potential candidate for many lightweight engineering applications with significant mechanical properties. In the view of the progressive literature reported in the field, this work aims to present the significance of natural fibers, their composites, and the main factors influencing these materials for various applications (automotive industry, for instance). Secondly, we aim to address different surface modifications and chemical treatments on natural fibers and finally provide an overview of natural fiber reinforced polymer composites’ potential applications.
Laser Coatings via State-of-the-Art Additive Manufacturing: A Review
Authors: Mahmood Muhammad , Bănică Alexandra , Ristoscu Carmen , Becherescu Nicu , Mihăilescu Ion
Published: March 2021

Ceramics and ceramic-reinforced metal matrix composites (CMMCs) demonstrate high wear resistance, excellent chemical inertness, and exceptional properties at elevated temperatures. These characteristics are suitable for their utilization in biomedical, aerospace, electronics, and other high-end engineering industries. The aforementioned performances make them difficult to fabricate via conventional manufacturing methods, requiring high costs and energy consumption. To overcome these issues, laser additive manufacturing (LAM) techniques, with high-power laser beams, were developed and extensively employed for processing ceramics and ceramic-reinforced CMMCs-based coatings. In respect to other LAM processes, laser melting deposition (LMD) excels in several aspects, such as high coating efficiency and lower labor cost. Nevertheless, difficulties such as poor bonding between coating and substrate, cracking, and reduced toughness are still encountered in some LMD coatings. In this article, we review recent developments in the LMD of ceramics and CMMCs-based coatings. Issues and solutions, along with development trends, are discussed and summarized in support of implementing this technology for current industrial use.
Software (GUI/APP) for Developing AI-Based Models Capable of Predicting Load-Displacement Curve and AFM Image during Nanoindentation
Authors: Jha Rajesh , Agarwal Arvind
Published: March 2021

During nanoindentation tests, the load-displacement curve is used for estimating mechanical properties, while an indent image obtained through atomic force microscopy (AFM) is used for studying deformation of a material. We present a computational platform for developing artificial intelligence-based models for predicting indentation depth (load-displacement curve) and AFM image as a function of test parameters like maximum applied load, loading rate, and holding time. A user can directly use machine generated data in text (.txt) and hierarchical data format (HDF, hdf) format for developing the AI-based models for indentation depth and AFM image, respectively. The software was tested on three different coatings/materials for indentation depth: heat-treated (HT) sample of cold sprayed aluminum-based bulk metallic glass (Al-BMG) coating, carbon nanotube reinforced aluminum composite (Al-5CNT) coating, and spark-plasma-sintered hydroxyapatite (SPS HA) sample. For AFM imaging, a heat-treated (HT) sample of cold sprayed aluminum-based bulk metallic glass (Al-BMG) coating was considered. Correlation or R-values are close to 1 for all the models developed in this work. Predicted load-displacement curve and AFM image are in good agreement with the experimental findings. Our approach will be helpful in virtual simulation of load-displacement curves and AFM indent images for a large number of new test parameters, thus significantly reducing the number of indents needed for characterizing/analyzing a material.
A Computer Vision Encyclopedia-Based Framework with Illustrative UAV Applications
Authors: Morley Terence , Morris Tim , Turner Martin
Published: March 2021

This paper presents the structure of an encyclopedia-based framework (EbF) in which to develop computer vision systems that incorporate the principles of agile development with focussed knowledge-enhancing information. The novelty of the EbF is that it specifies both the use of drop-in modules, to enable the speedy implementation and modification of systems by the operator, and it incorporates knowledge of the input image-capture devices and presentation preferences. This means that the system includes automated parameter selection and operator advice and guidance. Central to this knowledge-enhanced framework is an encyclopedia that is used to store all information pertaining to the current system operation and can be used by all of the imaging modules and computational runtime components. This ensures that they can adapt to changes within the system or its environment. We demonstrate the implementation of this system over three use cases in computer vision for unmanned aerial vehicles (UAV) showing how it is easy to control and set up by novice operators utilising simple computational wrapper scripts.
The Cryptographic Complexity of Anonymous Coins: A Systematic Exploration
Authors: Amarasinghe Niluka , Boyen Xavier , McKague Matthew
Published: March 2021

The modern financial world has seen a significant rise in the use of cryptocurrencies in recent years, partly due to the convincing lure of anonymity promised by these schemes. Bitcoin, despite being considered as the most widespread among all, is claimed to have significant lapses in relation to its anonymity. Unfortunately, studies have shown that many cryptocurrency transactions can be traced back to their corresponding participants through the analysis of publicly available data, to which the cryptographic community has responded by proposing new constructions with improved anonymity claims. Nevertheless, the absence of a common metric for evaluating the level of anonymity achieved by these schemes has led to numerous disparate ad hoc anonymity definitions, making comparisons difficult. The multitude of these notions also hints at the surprising complexity of the overall anonymity landscape. In this study, we introduce such a common framework to evaluate the nature and extent of anonymity in (crypto) currencies and distributed transaction systems, thereby enabling one to make meaningful comparisons irrespective of their implementation. Accordingly, our work lays the foundation for formalizing security models and terminology across a wide range of anonymity notions referenced in the literature, while showing how “anonymity” itself is a surprisingly nuanced concept, as opposed to existing claims that are drawn upon at a higher level, thus missing out on the elemental factors underpinning anonymity.
Boundary Conditions for Simulations of Fluid Flow and Temperature Field during Ammonothermal Crystal Growth—A Machine-Learning Assisted Study of Autoclave Wall Temperature Distribution
Authors: Schimmel Saskia , Tomida Daisuke , Saito Makoto , Bao Quanxi , Ishiguro Toru , Honda Yoshio , Chichibu Shigefusa , Amano Hiroshi
Published: March 2021

Thermal boundary conditions for numerical simulations of ammonothermal GaN crystal growth are investigated. A global heat transfer model that includes the furnace and its surroundings is presented, in which fluid flow and thermal field are treated as conjugate in order to fully account for convective heat transfer. The effects of laminar and turbulent flow are analyzed, as well as those of typically simultaneously present solids inside the autoclave (nutrient, baffle, and multiple seeds). This model uses heater powers as a boundary condition. Machine learning is applied to efficiently determine the power boundary conditions needed to obtain set temperatures at specified locations. Typical thermal losses are analyzed regarding their effects on the temperature distribution inside the autoclave and within the autoclave walls. This is of relevance because autoclave wall temperatures are a convenient choice for setting boundary conditions for simulations of reduced domain size. Based on the determined outer wall temperature distribution, a simplified model containing only the autoclave is also presented. The results are compared to those observed using heater-long fixed temperatures as boundary condition. Significant deviations are found especially in the upper zone of the autoclave due to the important role of heat losses through the autoclave head.
Fresco Paintings: Development of an Aging Model from 1064 nm Excited Raman Spectra
Authors: Pisu Francesca Assunta , Carbonaro Carlo Maria , Corpino Riccardo , Ricci Pier Carlo , Chiriu Daniele
Published: March 2021

In this study, we proposed a preliminary kinetic model applied to the carbonation process of fresh lime with the intention to realize a diagnostic tool for aged fresco paintings. The model can be useful, in particular, in the fields of conservation and restoration of ancient lime wall paintings. The dating procedure was achieved through the analysis of 1064 nm excited Raman spectra collected on artificially aged lime samples in addition to ancient samples taken from literature and covering a period of two thousand years. The kinetic model was developed monitoring the concentration of emitting defective centers related to the intensity of 780 cm−1 calcium hydroxide band as a function of the time and depth. This preliminary model shows how Raman spectroscopy, especially NIR micro-Raman, is advantageous for diagnostics and conservation in the cultural heritage field.
Prevalence and Diversity of Avian Haemosporidians May Vary with Anthropogenic Disturbance in Tropical Habitats in Myanmar
Authors: Muriel Jaime , Marzal Alfonso , Magallanes Sergio , García-Longoria Luz , Suarez-Rubio Marcela , Bates Paul J. J. , Lin Htet Htet , Soe Aye Nyein , Oo Khin Swe , Aye Aung Aung , Wilbur Naw Dolly , Win Ni Ni , Soe Yupa Tin , Linn Khaing Khin , Renner Swen C.
Published: March 2021

Avian malaria and related haemosporidians (genera Haemoproteus, Plasmodium and Leucocytozoon) infect most clades of bird. Although these parasites are present in almost all continents, they have been irregularly studied across different geographical regions. Despite the high bird diversity in Asia, the diversity of avian haemosporidians in this region is largely unknown. Moreover, anthropogenic changes to habitats in tropical regions may have a profound impact on the overall composition of haemosporidian communities. Here we analyzed the diversity and host association of bird haemosporidians from areas with different degrees of anthropogenic disturbance in Myanmar, revealing an unexplored diversity of these parasites (27% of newly-discovered haemosporidian lineages, and 64% of new records of host–parasite assemblages) in these tropical environments. This newly discovered diversity will be valuable for detecting host range and transmission areas of haemosporidian parasites. We also found slightly higher haemosporidian prevalence and diversity in birds from paddy fields than in individuals from urban areas and hills, thus implying that human alteration of natural environments may affect the dynamics of vector-borne diseases. These outcomes provide valuable insights for biodiversity conservation management in threatened tropical ecosystems.
A Pictorial Review of the Role of Imaging in the Detection, Management, Histopathological Correlations, and Complications of COVID-19 Pneumonia
Authors: Brogna Barbara , Bignardi Elio , Brogna Claudia , Volpe Mena , Lombardi Giulio , Rosa Alessandro , Gagliardi Giuliano , Capasso Pietro Fabio Maurizio , Gravino Enzo , Maio Francesca , Pane Francesco , Picariello Valentina , Buono Marcella , Colucci Lorenzo , Musto Lanfranco Aquilino
Published: March 2021

Imaging plays an important role in the detection of coronavirus (COVID-19) pneumonia in both managing the disease and evaluating the complications. Imaging with chest computed tomography (CT) can also have a potential predictive and prognostic role in COVID-19 patient outcomes. The aim of this pictorial review is to describe the role of imaging with chest X-ray (CXR), lung ultrasound (LUS), and CT in the diagnosis and management of COVID-19 pneumonia, the current indications, the scores proposed for each modality, the advantages/limitations of each modality and their role in detecting complications, and the histopathological correlations.
The Role of Transthoracic Ultrasound in the Study of Interstitial Lung Diseases: High-Resolution Computed Tomography Versus Ultrasound Patterns: Our Preliminary Experience
Authors: Lacedonia Donato , Scioscia Giulia , Giardinelli Angelamaria , Quarato Carla Maria Irene , Sassani Ennio Vincenzo , Foschino Barbaro Maria Pia , Maci Federica , Sperandeo Marco
Published: March 2021

Transthoracic ultrasound (TUS) is a readily available imaging tool that can provide a quick real-time evaluation. The aim of this preliminary study was to establish a complementary role for this imaging method in the approach of interstitial lung diseases (ILDs). TUS examination was performed in 43 consecutive patients with pulmonary fibrosis and TUS findings were compared with the corresponding high-resolution computed tomography (HRCT) scans. All patients showed a thickened hyperechoic pleural line, despite no difference between dominant HRCT patterns (ground glass, honeycombing, mixed pattern) being recorded (p > 0.05). However, pleural lines’ thickening showed a significant difference between different HRCT degree of fibrosis (p < 0.001) and a negative correlation with functional parameters. The presence of >3 B-lines and subpleural nodules was also assessed in a large number of patients, although they did not demonstrate any particular association with a specific HRCT finding or fibrotic degree. Results allow us to suggest a complementary role for TUS in facilitating an early diagnosis of ILD or helping to detect a possible disease progression or eventual complications during routine clinical practice (with pleural line measurements and subpleural nodules), although HRCT remains the gold standard in the definition of ILD pattern, disease extent and follow-up.
Ethical Implications of Alzheimer’s Disease Prediction in Asymptomatic Individuals through Artificial Intelligence
Authors: Ursin Frank , Timmermann Cristian , Steger Florian
Published: March 2021

Biomarker-based predictive tests for subjectively asymptomatic Alzheimer’s disease (AD) are utilized in research today. Novel applications of artificial intelligence (AI) promise to predict the onset of AD several years in advance without determining biomarker thresholds. Until now, little attention has been paid to the new ethical challenges that AI brings to the early diagnosis in asymptomatic individuals, beyond contributing to research purposes, when we still lack adequate treatment. The aim of this paper is to explore the ethical arguments put forward for AI aided AD prediction in subjectively asymptomatic individuals and their ethical implications. The ethical assessment is based on a systematic literature search. Thematic analysis was conducted inductively of 18 included publications. The ethical framework includes the principles of autonomy, beneficence, non-maleficence, and justice. Reasons for offering predictive tests to asymptomatic individuals are the right to know, a positive balance of the risk-benefit assessment, and the opportunity for future planning. Reasons against are the lack of disease modifying treatment, the accuracy and explicability of AI aided prediction, the right not to know, and threats to social rights. We conclude that there are serious ethical concerns in offering early diagnosis to asymptomatic individuals and the issues raised by the application of AI add to the already known issues. Nevertheless, pre-symptomatic testing should only be offered on request to avoid inflicted harm. We recommend developing training for physicians in communicating AI aided prediction.
Twin-to-Twin Transfusion Syndrome: Diagnostic Imaging and Its Role in Staving Off Malpractice Charges and Litigation
Authors: Zaami Simona , Masselli Gabriele , Brunelli Roberto , Taschini Giulia , Caprasecca Stefano , Marinelli Enrico
Published: March 2021

The study aims to expound upon the imaging-based diagnostic methodologies aimed at identifying twin-to-twin transfusion syndrome (TTTS), a serious, somewhat rare prenatal condition that takes place in pregnancies where identical twins, or other multiples, share a placenta (monochorionic placenta), highlighting how medico-legal outcomes can be affected by provable compliance with consolidated diagnostic guidelines or best practices. It is of utmost importance to produce a prompt identification of TTTS instances; an early diagnosis is in fact critical in order to effectively treat and manage TTTS. By virtue of TTTS being a highly progressive condition, a delay in diagnosis can result in disastrous outcomes; just a few weeks delay in the diagnosis of TTTS can turn out fatal for one or both twins. Hence, most TTTS malpractice claims involve allegations of medical negligence, namely the failure to recognize the condition in a timely fashion, or to proceed with adequate diagnostic and therapeutic pathways. In that regard, case law databases have been pored over (Justia, Lexis, Leagle), and five significant court cases have been examined and discussed in an attempt to identify objective medico-legal standards and bring to the forefront relevant forensic dynamics. In fact, when health professionals are capable of proving adherence to guidelines or best practices, this can shield them from malpractice allegations and ensuing litigation.
Rare Causes of Arterial Hypertension and Thoracic Aortic Aneurysms—A Case-Based Review
Authors: Encica Svetlana , Molnar Adrian , Manole Simona , Filan Teodora , Oprița Simona , Bursașiu Eugen , Vulturar Romana , Damian Laura
Published: March 2021

Thoracic aortic aneurysms may result in dissection with fatal consequences if undetected. A young male patient with no relevant familial history, after having been investigated for hypertension, was diagnosed with an ascending aortic aneurysm involving the aortic root and the proximal tubular segment, associated with a septal atrial defect. The patient underwent a Bentall surgery protocol without complications. Clinical examination revealed dorso–lumbar scoliosis and no other signs of underlying connective tissue disease. Microscopic examination revealed strikingly severe medial degeneration of the aorta, with areas of deep disorganization of the medial musculo–elastic structural units and mucoid material deposition. Genetic testing found a variant of unknown significance the PRKG1 gene encoding the protein kinase cGMP-dependent 1, which is important in blood pressure regulation. There may be genetic links between high blood pressure and thoracic aortic aneurysm determinants. Hypertension was found in FBN1 gene mutations encoding fibrillin and in PRKG1 mutations. Possible mechanisms involving the renin–angiotensin system, the role of oxidative stress, osteopontin, epigenetic modifications and other genes are reviewed. Close follow-up and strict hypertension control are required to reduce the risk of dissection. Hypertension, scoliosis and other extra-aortic signs suggesting a connective tissue disease are possible clues for diagnosis.
Occult Disco-Ligamentous Lesions of the Subaxial c-Spine—A Comparison of Preoperative Imaging Findings and Intraoperative Site Inspection
Authors: Janssen Insa , Sollmann Nico , Barz Melanie , Baum Thomas , Schaller Karl , Zimmer Claus , Ryang Yu-Mi , Kirschke Jan S. , Meyer Bernhard
Published: March 2021

Despite the general acceptance of magnetic resonance imaging (MRI) as the gold standard for diagnostics of traumatic disco-ligamentous injuries in the subaxial cervical spine, clinical experience shows cases where no lesion is detected in MRI exams but obtained during surgery. The aim of this study was to compare intraoperative site inspection to preoperative imaging findings and to identify radiological features of patients having a risk for under- or over-estimating disco-ligamentous lesions. We performed a retrospective analysis of our clinical database, considering all patients who underwent surgical treatment of the cervical spine via an anterior approach after trauma between June 2008 and April 2018. Only patients with availability of immediate preoperative computed tomography (CT), 3-Tesla MRI scans, and information about intraoperative findings were considered. Results of preoperative imaging were set in context to intraoperative findings, and receiver operator characteristics (ROC) were calculated. Out of 144 patients receiving anterior cervical surgery after trauma, 83 patients (mean age: 59.4 ± 20.5 years, age range: 12–94 years, 63.9% males) were included in this study. Included patients underwent surgical treatment via anterior cervical discectomy and fusion (ACDF; 79 patients) or anterior cervical corpectomy and fusion (4 patients) with ventral plating. Comparing preoperative imaging findings to intraoperative site inspection, a discrepancy between imaging and surgical findings was revealed in 14 patients, leading to an overall specificity/sensitivity of preoperative imaging to identify disco-ligamentous lesions of the cervical spine of 100%/77.4%. Yet, adding the existence of prevertebral hematoma and/or vertebral fractures according to preoperative imaging improved the sensitivity to 95.2%. Lack of sensitivity was most likely related to severe cervical spondylosis, rendering correct radiological reporting difficult. Thus, the risk of missing a traumatic disco-ligamentous injury of the cervical spine in imaging seems to be a particular threat in patients with preexisting degenerative cervical spondylosis. In conclusion, incorporating the existence of prevertebral hematoma and/or vertebral fractures can significantly improve diagnostic yield.
Latest Insights into Mechanisms behind Atrial Cardiomyopathy: It Is Not always about Ventricular Function
Authors: Dmour Bianca-Ana , Miftode Radu-Stefan , Iliescu Halitchi Dan , Anton-Paduraru Dana Teodora , Iliescu Halitchi Codruta-Olimpiada , Miftode Ionela-Larisa , Mitu Ovidiu , Costache Alexandru-Dan , Stafie Celina-Silvia , Costache Irina Iuliana
Published: March 2021

Atrial cardiomyopathy (ACM) represents a constantly evolving concept, with increasing importance in contemporary research and clinical practice. A better understanding of the mechanisms involved in atrial remodeling and its clinical correlations especially with atrial fibrillation (AF) and other cardiometabolic comorbidities may induce a significant impact on the diagnosis, prognosis, and therapeutic approach of ACM-related comorbidities. Although initially described several decades ago, investigators have only recently highlighted that several renal, metabolic, and cardiovascular diseases are determining factors for atrial remodeling and subsequent ACM. Based on data from multiple recent studies, our research emphasizes the correlations between ACM and other coexisting pathologies including cardiovascular, respiratory, or metabolic diseases, with fibrosis being the most incriminated pathophysiological mechanism. In addition to the usual tests, the paraclinical assessment of ACM is increasingly based on the use of various cardiac biomarkers, while the cardiac magnetic resonance (CMR) has become an increasingly tempting diagnostic too for describing morphofunctional aspects of the heart chambers, with the gadolinium contrast enhanced CMR (LGE-CMR) emerging as a commonly used technique aiming to identify and quantify the precise extent of atrial fibrosis. Further research should be conducted in order to clarify our knowledge regarding atrial remodeling and, therefore, to develop new and improved therapeutic approaches in these patients.
Do the Peculiar Economics of Professional Team Sports Apply to Esports? Sequential Snowballing Literature Reviews and Implications
Authors: Scelles Nicolas , Peng Qi , Valenti Maurizio
Published: March 2021

The present research aims to identify the main peculiar economics of professional team sports, reflect on whether they apply to esports, and derive some implications. To achieve this aim, two sequential snowballing literature reviews were conducted. First, the literature on the peculiar economics of professional team sports was reviewed and assessed by the authors, based on their degree of distinctiveness and how core they are for the sector. Second, based on the main peculiar economics identified, a similar process considering economic aspects in the esports literature was conducted. The first review enabled the identification of 50 peculiar economics of professional team sports, of which 12 were assessed as the most distinctive and core to the sector. These 12 main peculiar economics were then considered in relation to the esports literature. This second review enabled the identification of some economic similarities and differences between sports and esports, before deriving some implications.
Evaluating the Quality of Machine Learning Explanations: A Survey on Methods and Metrics
Authors: Zhou Jianlong , Gandomi Amir H. , Chen Fang , Holzinger Andreas
Published: March 2021

The most successful Machine Learning (ML) systems remain complex black boxes to end-users, and even experts are often unable to understand the rationale behind their decisions. The lack of transparency of such systems can have severe consequences or poor uses of limited valuable resources in medical diagnosis, financial decision-making, and in other high-stake domains. Therefore, the issue of ML explanation has experienced a surge in interest from the research community to application domains. While numerous explanation methods have been explored, there is a need for evaluations to quantify the quality of explanation methods to determine whether and to what extent the offered explainability achieves the defined objective, and compare available explanation methods and suggest the best explanation from the comparison for a specific task. This survey paper presents a comprehensive overview of methods proposed in the current literature for the evaluation of ML explanations. We identify properties of explainability from the review of definitions of explainability. The identified properties of explainability are used as objectives that evaluation metrics should achieve. The survey found that the quantitative metrics for both model-based and example-based explanations are primarily used to evaluate the parsimony/simplicity of interpretability, while the quantitative metrics for attribution-based explanations are primarily used to evaluate the soundness of fidelity of explainability. The survey also demonstrated that subjective measures, such as trust and confidence, have been embraced as the focal point for the human-centered evaluation of explainable systems. The paper concludes that the evaluation of ML explanations is a multidisciplinary research topic. It is also not possible to define an implementation of evaluation metrics, which can be applied to all explanation methods.
ColorWatch: Color Perceptual Spatial Tactile Interface for People with Visual Impairments
Authors: Jabbar Muhammad Shahid , Lee Chung-Heon , Cho Jun Dong
Published: March 2021

Tactile perception enables people with visual impairments (PVI) to engage with artworks and real-life objects at a deeper abstraction level. The development of tactile and multi-sensory assistive technologies has expanded their opportunities to appreciate visual arts. We have developed a tactile interface based on the proposed concept design under considerations of PVI tactile actuation, color perception, and learnability. The proposed interface automatically translates reference colors into spatial tactile patterns. A range of achromatic colors and six prominent basic colors with three levels of chroma and values are considered for the cross-modular association. In addition, an analog tactile color watch design has been proposed. This scheme enables PVI to explore artwork or real-life object color by identifying the reference colors through a color sensor and translating them to the tactile interface. The color identification tests using this scheme on the developed prototype exhibit good recognition accuracy. The workload assessment and usability evaluation for PVI demonstrate promising results. This suggest that the proposed scheme is appropriate for tactile color exploration.
Monitoring Forest Phenology in a Changing World
Authors: Gray Ross E. J. , Ewers Robert M.
Published: March 2021

Plant phenology is strongly interlinked with ecosystem processes and biodiversity. Like many other aspects of ecosystem functioning, it is affected by habitat and climate change, with both global change drivers altering the timings and frequency of phenological events. As such, there has been an increased focus in recent years to monitor phenology in different biomes. A range of approaches for monitoring phenology have been developed to increase our understanding on its role in ecosystems, ranging from the use of satellites and drones to collection traps, each with their own merits and limitations. Here, we outline the trade-offs between methods (spatial resolution, temporal resolution, cost, data processing), and discuss how their use can be optimised in different environments and for different goals. We also emphasise emerging technologies that will be the focus of monitoring in the years to follow and the challenges of monitoring phenology that still need to be addressed. We conclude that there is a need to integrate studies that incorporate multiple monitoring methods, allowing the strengths of one to compensate for the weaknesses of another, with a view to developing robust methods for upscaling phenological observations from point locations to biome and global scales and reconciling data from varied sources and environments. Such developments are needed if we are to accurately quantify the impacts of a changing world on plant phenology.
Growth of Listeria monocytogenes in Partially Cooked Battered Chicken Nuggets as a Function of Storage Temperature
Authors: Lianou Alexandra , Raftopoulou Ourania , Spyrelli Evgenia , Nychas George-John E.
Published: March 2021

Battered poultry products may be wrongly regarded and treated by consumers as ready-to-eat and, as such, be implicated in foodborne disease outbreaks. This study aimed at the quantitative description of the growth behavior of Listeria monocytogenes in fresh, partially cooked (non-ready-to-eat) battered chicken nuggets as function of temperature. Commercially prepared chicken breast nuggets were inoculated with L. monocytogenes and stored at different isothermal conditions (4, 8, 12, and 16 °C). The pathogen’s growth behavior was characterized via a two-step predictive modelling approach: estimation of growth kinetic parameters using a primary model, and description of the effect of temperature on the estimated maximum specific growth rate (μmax) using a secondary model. Model evaluation was undertaken using independent growth data under both constant and dynamic temperature conditions. According to the findings of this study, L. monocytogenes may proliferate in battered chicken nuggets in the course of their shelf life to levels potentially hazardous for susceptible population groups, even under well-controlled refrigerated storage conditions. Model evaluation demonstrated a satisfactory performance, where the estimated bias factor (Bf) was 0.92 and 1.08 under constant and dynamic temperature conditions, respectively, while the accuracy factor (Af) value was 1.08, in both cases. The collected data should be useful in model development and quantitative microbiological risk assessment in battered poultry products.
Replacement of Sodium Tripolyphosphate with Iota Carrageenan in the Formulation of Restructured Ostrich Ham
Authors: Schutte Sumari , Marais Jeannine , Muller Magdalena , Hoffman Louwrens C.
Published: March 2021

The influence of iota carrageenan (iota-CGN) as a partial replacement of sodium tripolyphosphate (STPP) was investigated on the physical (pH, yield, instrumental color, texture profile analysis), chemical (moisture, protein, total fat, ash, phosphate) and sensory (descriptive analysis, acceptance testing) quality of restructured ostrich ham (95% lean meat plus fat). Treatments consisted of five decreasing levels of STPP (0.70%, 0.53%, 0.35%, 0.18% and 0%) that were simultaneously substituted with five increasing levels of iota-CGN (0%, 0.1%, 0.2%, 0.3% and 0.4%). Cooked yield, hardness, cohesiveness, and gumminess of restructured ostrich ham increased (p ≤ 0.05) with decreasing levels of STPP (and increased levels of iota-CGN). No significant trend in instrumental color measurements or springiness were observed between treatments. Ostrich ham with 0.35% STPP and lower had increased ostrich meat aroma and flavor, while spicy aroma and flavor, mealiness and consumer acceptance decreased. Iota carrageenan can be substituted for STPP (up to 0.35% STPP and 0.2% iota-CGN) to produce reduced STPP ham.
Resistant Starch Combined with Whey Protein Increases Postprandial Metabolism and Lowers Glucose and Insulin Responses in Healthy Adult Men
Authors: Mohr Alex E. , Minicucci Olivia , Long Dale , Miller Vincent J. , Keller Allison , Sheridan Caitlin , O’brien Gabriel , Ward Emery , Schuler Brad , Connelly Scott , Holst Jens J. , Astrup Arne , He Feng , Gentile Christopher L. , Arciero Paul J.
Published: March 2021

Resistant starch (RS) and/or protein consumption favorably influence energy metabolism, substrate utilization, and weight management. The current study administered four different versions of a pancake breakfast containing waxy maize or RS with and without whey protein (WP) and measured postprandial thermogenesis (TEM), fuel utilization, and circulating satiation and appetite factors for 180 min in a group of healthy, adult men. On four separate visits to the laboratory, eight participants were administered four different pancake breakfast meal challenges using a single-blind, randomized crossover design: (1) waxy maize starch (WMS) control; (2) WMS and WP (WMS + WP); (3) RS; or (4) RS and WP (RS + WP). TEM (kcals/180 min) was significantly greater (p < 0.05) in RS + WP (45.11; confidence interval (CI), 33.81–56.41) compared to WMS (25.61; CI, 14.31–36.91), RS (29.44; CI, 18.14–40.74), and WMS + WP (24.64; CI, 13.34–35.94), respectively. Fat oxidation was enhanced (p < 0.05) after RS + WP compared to RS at 60 min (+23.10%), WMS at 120 min (+27.49%), and WMS and WMS + WP at 180 min (+35.76%; +17.31%, respectively), and RER was decreased with RS + WP versus the other three meals (mean differences: ≥−0.021). Insulin concentrations were decreased (p < 0.05) following RS + WP compared to WMS, whereas both RS (−46.19%) and RS + WP (−53.05%) insulin area under the curve (AUC) were greatly reduced (p < 0.01) compared to WMS. While limited by sample size, meals containing both RS and WP increased postprandial thermogenesis and fat oxidation, and lowered insulin response compared to isocaloric meals without this combination. Therefore, RS + WP may favorably impact energy metabolism and thus weight control and body composition under chronic feeding conditions.
Molecular Profiling and Gene Banking of Rabbit EPCs Derived from Two Biological Sources
Authors: Vašíček Jaromír , Baláži Andrej , Bauer Miroslav , Svoradová Andrea , Tirpáková Mária , Tomka Marián , Chrenek Peter
Published: March 2021

Endothelial progenitor cells (EPCs) have been broadly studied for several years due to their outstanding regenerative potential. Moreover, these cells might be a valuable source of genetic information for the preservation of endangered animal species. However, a controversy regarding their characterization still exists. The aim of this study was to isolate and compare the rabbit peripheral blood- and bone marrow-derived EPCs with human umbilical vein endothelial cells (HUVECs) in terms of their phenotype and morphology that could be affected by the passage number or cryopreservation as well as to assess their possible neuro-differentiation potential. Briefly, cells were isolated and cultured under standard endothelial conditions until passage 3. The morphological changes during the culture were monitored and each passage was analyzed for the typical phenotype using flow cytometry, quantitative real–time polymerase chain reaction (qPCR) and novel digital droplet PCR (ddPCR), and compared to HUVECs. The neurogenic differentiation was induced using a commercial kit. Rabbit cells were also cryopreserved for at least 3 months and then analyzed after thawing. According to the obtained results, both rabbit EPCs exhibit a spindle-shaped morphology and high proliferation rate. The both cell lines possess same stable phenotype: CD14−CD29+CD31−CD34−CD44+CD45−CD49f+CD73+CD90+CD105+CD133−CD146−CD166+VE-cadherin+VEGFR-2+SSEA-4+MSCA-1−vWF+eNOS+AcLDL+ALDH+vimentin+desmin+α-SMA+, slightly different from HUVECs. Moreover, both induced rabbit EPCs exhibit neuron-like morphological changes and expression of neuronal markers ENO2 and MAP2. In addition, cryopreserved rabbit cells maintained high viability (>85%) and endothelial phenotype after thawing. In conclusion, our findings suggest that cells expanded from the rabbit peripheral blood and bone marrow are of the endothelial origin with a stable marker expression and interesting proliferation and differentiation capacity.