bluuebunny/crossref_metadata_2025 huggingface dataset:
Rates and Determinants of Retention on ART Among Orphans and Vulnerable Children Living With HIV in Tanzania
Authors: Charles John , Exavery Amon , Ally Amal , Mseya Remmy , Mbwambo Tumainiel , Barankena Asheri , Kyaruzi Christina , Kikoyo Levina
Published: July 2022

Despite the global progress in response to HIV and AIDS, notable challenges remain for children, especially identification, linkage, and retention in HIV care and treatment services. To succeed in pediatric HIV programming requires the linkage and retention of the children in those services over time. This study assessed the level of retention to antiretroviral therapy (ART) and its associated factors among orphans and vulnerable children living with HIV (OVCLHIV) in Tanzania. Data were obtained from the USAID Kizazi Kipya project that collected pediatric ART data from October 2017 to October 2019 in 81 district councils of Tanzania. Community-based volunteers supported the linkage and retention of the OVCLHIV on ART. Analysis of on-ART status was conducted in a cohort of OVCLHIV aged 0–20 years enrolled in the project and monitored for 24 months. OVCLHIV who remained on ART until the end of the follow-up period were referred to as “retained,” otherwise, “not retained”. Multivariable analysis was conducted using logistic regression, adjusting for baseline characteristics. Of the 5,304 OVCLHIV analyzed, the mean age was 13.1 years, 51.5% were female, and 72.2% were living with female caregivers. Their overall rate of retention on ART over the 24 months was 86.7%. Multivariable analysis showed that as the higher frequency of home visit by the project staff increased, the likelihood of retention increased by 8% [adjusted odds ratio (aOR) = 1.08, 95% CI 1.06–1.11, p < 0.001]. Membership in people living with HIV (PLHIV) support groups was associated with a higher likelihood of retention compared to nonmembership (aOR = 3.31, 95% CI 2.60–4.21, p < 0.001). Children in larger family size were 22% less likely to sustain ART (aOR = 0.78, 95% CI 0.72–0.84, p < 0.001). Urban OVCLHIV were 18% less likely to remain on ART than their rural counterparts (aOR = 0.82, 95% CI 0.69–0.98, p = 0.030). Remaining on ART was 49% more likely for OVC in economically better-off households than those in destitute households (aOR = 1.49, 95% CI 1.22–1.81, p < 0.001). Male OVC were 17% less likely to be retained on ART than their female counterparts (aOR = 0.83, 95% CI 0.71–0.99, p = 0.033). Community-based OVC support resulted in a high pediatric retention rate over the 24 months of follow-up. While key enablers of retention were higher frequency of home visits by the project volunteer, participation in PLHIV support groups, and better economic status, large family sizes, urban place of residence, and male gender of the OVC were barriers. This study brings useful evidence to inform strategies for advancing retention of OVCLHIV on ART for their better health outcomes and overall wellbeing.
Knowledge, attitude, and practice towards face mask use among residents of Greater Chennai Corporation, India, March 2021
Authors: Nagarajan Ramya , Rubeshkumar Polani , Jagadeesan Murugesan , Raju Mohankumar , Sakthivel Manikandanesan , Murali Sharan , Sendhilkumar Muthappan , Ilangovan Kumaravel , Harikrishnan Dineshkumar , Venkatasamy Vettrichelvan , Ganeshkumar Parasuraman , Kaur Prabhdeep
Published: July 2022

Background

Wearing a mask is one of the simplest ways to reduce the spread of COVID-19. Studies reported poor mask compliance in Greater Chennai Corporation, India. Hence, we described the knowledge, attitude, and practice regarding mask use among adults (≥18 years) in Greater Chennai Corporation, Tamil Nadu, India.

Methods

We conducted a cross-sectional survey among residents of Greater Chennai Corporation in March 2021. We estimated the sample size to be 203 per strata (slum and non-slum). We used a simple random sampling technique to select 20 locations using a digital map in the slum and non-slum areas. After reaching the location chosen, we selected 10 consecutive households and one adult (≥18 years of age) from each household. We used a validated, semi-structured questionnaire for collecting data regarding knowledge, attitudes, and practices for mask use. We estimated proportions and 95% CI for key variables and compared the variables between slums and non-slums.

Results

Of 430 participants included in the study, 51.4% were males. The mean (S.D.) age of the participants is 41.1 (14.6) years. The majority (86.7%) of the participants felt that wearing a mask helped in reducing the spread of coronavirus and the knowledge differed (p-value < 0.05) between the slum (81.4%) and non-slum (92.3%). Nearly half (46.5%) of the participants did not like being forced to wear the mask. About 63.9% of the participants reported the practice of mask use while going out which was similar across slums and non-slums.

Conclusion

Although the knowledge regarding mask use was good among the public, the attitude was unfavorable. We suggest continuous reinforcement by spreading awareness and educating the community on the appropriate use of the mask.
Assessing Canadian women's preferences for cervical cancer screening: A brief report
Authors: Zhu Patricia , Tatar Ovidiu , Haward Ben , Griffin-Mathieu Gabrielle , Perez Samara , Smith Laurie , Brotherton Julia , Ogilvie Gina , Rosberger Zeev
Published: July 2022

Human papillomavirus (HPV) testing is recommended for primary screening for cervical cancer by several health authorities. Several countries that have implemented HPV testing programs have encountered resistance against extended screening intervals and older age of initiation. As Canada prepares to implement HPV testing programs, it is important to understand women's preferences toward cervical cancer screening to ensure a smooth transition. The objective of this study was to assess Canadian women's current preferences toward cervical cancer screening. Using a web-based survey, we recruited underscreened ( > 3 years since last Pap test) and adequately screened (< 3 years since last Pap test) Canadian women aged 21–70 who were biologically female and had a cervix. We used Best-Worst Scaling (BWS) methodology to collect data on women's preferences for different screening methods, screening intervals, and ages of initiation. We used conditional logistic regression to estimate preferences in both subgroups. In both subgroups, women preferred screening every three years compared to every five or ten years, and initiating screening at age 21 compared to age 25 or 30. Adequately screened women (n = 503) most preferred co-testing, while underscreened women (n = 524) preferred both co-testing and HPV self-sampling over Pap testing. Regardless of screening status, women preferred shorter screening intervals, an earlier age of initiation, and co-testing. Adequate communication from public health authorities is needed to explain the extended screening intervals and age of initiation to prevent resistance against these changes to cervical cancer screening.
Osteoradionecrosis of the jaw: A mini review
Authors: Singh Annu , Huryn Joseph M. , Kronstadt Kenneth L. , Yom SaeHee K. , Randazzo Joseph R. , Estilo Cherry L.
Published: July 2022

Osteoradionecrosis (ORN) of the jaw is one of the most dreaded complications of head and neck radiation therapy. Despite the evolution of radiation treatment modalities, ORN continues to remain a therapeutic challenge and its etiopathogenesis still remains unclear. It is clinically characterized by exposed necrotic bone within the head and neck radiation field. Over the past years, several studies have reported on the definition, staging, incidence, etiology, and management of this oral complication. In this review, we summarize the literature on ORN and discuss our institutional experience and management strategies that aim to predict and mitigate risk for ORN.
Microbiological and behavioral determinants of genital HPV infections among adolescent girls and young women warrant the need for targeted policy interventions to reduce HPV risk
Authors: Onywera Harris , Mabunda Sikhumbuzo A. , Williamson Anna-Lise , Mbulawa Zizipho Z. A.
Published: July 2022

Background

Genital human papillomavirus (HPV) is the most common sexually transmitted virus in most populations globally. Adolescent girls and young women (AGYW) remain a key population group at risk for HPV infection. However, the risk factors of HPV infection among AGYW, especially in sub-Saharan Africa, are a subject of little investigation in published literature. Here, we investigated the factors associated with HPV infection among unvaccinated South African AGYW with a high HPV burden (prevalence: 76.1%).

Methods

We retrospectively recruited 213 AGYW learners (aged 15–25 years) from a previous cross-sectional study, the HPV Education Intervention Study, conducted in the Eastern Cape, South Africa. Sexually transmitted infections (STIs), bacterial pathobionts, genital ulcers (due to infectious causes), candidiasis, and bacterial vaginosis (BV) in the self-collected vaginal specimens were determined using the Allplex™ Panel Assays. Statistical analyses were performed using STATA v16.1. Continuous and categorical variables were computed by t-test /Wilcoxon rank-sum test and Chi-square/Fisher's exact tests, respectively. Logistic regression was used to determine the univariable predictors of HPV infection.

Results

The overall detection rate of any viral STI, bacterial STI, pathobiont, genital ulcer, candidiasis, and BV among the AGYW was 75.0, 34.4, 90.7, 14.4, 26.9, and 43.6%, respectively. The main factors associated with HPV infection were alcohol consumption (p = 0.005), infection with any and multiple Candida species (p = 0.011 and 0.006, respectively), Candida albicans infection (p = 0.010), Ureaplasma urealyticum pathobiont infection (p = 0.044), BV-associated bacteria (specifically Atopobium vaginae: p = 0.039, BV-associated bacteria 2: p = 0.021, Gardnerella vaginalis: p = 0.021, Megasphaera type 1: p = 0.037), and BV (p = 0.011).

Conclusions

Our study, albeit not necessarily generalizable, found social behavior as well as specific vaginal microbes as correlates of HPV infection among AGYW in South Africa. There is a need to investigate HPV epidemiology in other AGYW populations. The factors associated with genital HPV infection among AGYW burdened with HPV infection necessitate the need to formulate and implement population-specific public health strategies for creating HPV awareness and reducing its risk.
Life-cycle assessment of hydrogen utilization in power generation: A systematic review of technological and methodological choices
Authors: Rinawati Dyah Ika , Keeley Alexander Ryota , Takeda Shutaro , Managi Shunsuke
Published: July 2022

Interest in reducing the greenhouse gas emissions from conventional power generation has increased the focus on the potential use of hydrogen to produce electricity. Numerous life-cycle assessment (LCA) studies of hydrogen-based power generation have been published. This study reviews the technological and methodological choices made in hydrogen-based power generation LCAs. A systematic review was chosen as the research method to achieve a comprehensive and minimally biased overview of hydrogen-based power generation LCAs. Relevant articles published between 2004 and 2021 were identified by searching the Scopus and Web of Science databases. Electrolysis from renewable energy resources was the most widely considered type of hydrogen production in the LCAs analyzed. Fuel cell technology was the most common conversion equipment used in hydrogen-based electricity LCAs. A significant number of scenarios examine the use of hydrogen for energy storage and co-generation purposes. Based on qualitative analysis, the methodological choices of LCAs vary between studies in terms of the functional units, allocations, system boundaries, and life-cycle impact assessment methods chosen. These discrepancies were likely to influence the value of the environmental impact results. The findings of the reviewed LCAs could provide an environmental profile of hydrogen-based electricity systems, identify hotspots, drive future research, define performance goals, and establish a baseline for their large-scale deployment.
What’s the Buzz About Native Bees?
Authors: Rykken Jessica
Published: July 2022

Most plants depend on insects for pollination. Honey bees pollinate many of the foods people eat, but did you know that wild plants, and animals like birds and bears, also depend on pollinators? Native bees are the most diverse and efficient pollinators. Thousands of bee species transport pollen between plants in deserts, forests, mountains, meadows, and many other habitats. This service helps plants reproduce successfully, and the plants provide food and shelter for other animals. Bees are important for keeping our wild landscapes healthy. Scientists are discovering that climate change and other human-caused threats are changing bee populations. Therefore, it is important that we learn more about pollinators in wild places like national parks and that we support bees in our own backyards.
Assessing the prevalence, sources and selective antecedents of organizational stressors among elite football players and coaches in the Ghana premier league: Empirical evidence for applied practice
Authors: Srem-Sai Medina , Hagan John Elvis , Ogum Prosper Narteh , Schack Thomas
Published: July 2022

Globally, job-related stress has been classified as a health epidemic which is common among many individuals across diverse populations. Despite this established knowledge, research has primarily focused on the general population and among health workers. Therefore, understanding stress related experiences in the context of professional sport would help design appropriate stress management interventions for effective coping. The overarching aim of this research was to assess occupational stress related experiences among players and coaches in the Ghana premier league. The study sought to assess: (1) the prevalence and sources of stressors among players and coaches, and (2) how age and years of experience influenced the stressors they experienced. Using a census survey, 44 premier league coaches and 424 players who were officially registered by 17 premier league clubs completed the intensity dimension of the Organizational Stressor Indicator for Sport Performers which has 5 subscales namely: Goals and Development, Logistics and Operations, Team and Culture, Coaching and Selection. Descriptive statistics (means and standard deviation) and multivariate analysis of variance were used to analyze the data. The results revealed that stressors were prevalent among football players and coaches, with these being significantly more dominant among coaches, p < 0.001. Selection was identified as the most predominant stressor for coaches (i.e., selecting players to play) and players (i.e., being selected). Age and experience were found not to be significant predictors of stressors for players and coaches in Ghana. Findings suggest that generally, stressors are common among football players and coaches, especially on issues related to selection. Sport psychologists and team managers in the various premier league clubs should incorporate appropriate interventions (e.g., stress inoculation training) aimed at providing adequate psychological support to promote players' and coaches' wellbeing.
Clinical effects of unilateral biportal endoscopic decompression for lumbar posterior apophyseal ring separation
Authors: Liu Jianjun , Zhu Bin , Chen Lei , Jing Juehua , Tian Dasheng
Published: July 2022

Objective

The purpose of the study was to investigate the feasibility and effects of unilateral biportal endoscopic decompression for lumbar posterior apophyseal ring separation (PARS).

Methods

Patients with lumbar PARS who received unilateral biportal endoscopic decompression from June 2020 to September 2021 were analyzed, including 11 females and 15 males. The clinical symptoms were consistent with the imaging findings. Operation time, length of postoperative hospital stay and complications were recorded, and the clinical efficacy was evaluated by Visual Analogue Scale (VAS), Oswestry Disability Index (ODI) and modified Macnab scale at preoperative, postoperative 1, 3, 6 months and the last follow-up.

Results

Preoperative VAS scores of low back pain were (5.04 ± 1.37) and respectively decreased to (2.81 ± 0.75), (2.35 ± 0.98), (1.65 ± 0.69) and (1.15 ± 0.68) at postoperative 1, 3, 6 months and at the last follow-up, and the difference was statistically significant (F = 127.317, P = 0.000). Preoperative VAS scores of lower limb pain were (6.92 ± 1.38) and respectively decreased to (2.88 ± 1.07), (2.54 ± 1.03), (1.81 ± 0.80) and (1.00 ± 0.69) at postoperative 1, 3, 6 months and at the last follow-up, and the difference was statistically significant (F = 285.289, P = 0.000). Preoperative ODI scores were (60.47 ± 8.89) and respectively decreased to (34.72 ± 4.13), (25.80 ± 3.65), (17.71 ± 3.41) and (5.65 ± 2.22) at postoperative 1, 3, 6 months and at the last follow-up, and the difference was statistically significant (F = 725.255, P = 0.000). According to the modified Macnab criteria, the final outcome was excellent in 22 cases, good in 3 cases, fair in 1 cases. 26 patients could return to work or normal activities within 3 weeks.

Conclusions

Unilateral biportal endoscopic decompression has the advantages of clear and wide field of vision, large operating space, relatively simple need of surgical instrument and convenient and flexible operation procedure. It can achieve excellent clinical results with favorable efficacy and safety and may become a new minimally invasive endoscopic treatment for lumbar PARS.
Identifying octogenarians with non-small cell lung cancer who could benefit from surgery: A population-based predictive model
Authors: Chao Ce , Di Dongmei , Wang Min , Liu Yang , Wang Bin , Qian Yongxiang
Published: July 2022

Background

As the population ages, there will be an increasing number of octogenarian patients with non-small cell lung cancer (NSCLC). In carefully selected elderly patients, surgery can improve long-term survival. To identify candidates who would benefit from surgery, we performed this study and built a predictive model.

Materials and methods

Data from NSCLC patients over 80 years old were obtained from the Surveillance, Epidemiology and End Results database. A 1:1 propensity score matching was performed to balance the clinicopathological features between the surgery and non-surgery groups. Kaplan-Meier analyses and log-rank tests were used to assess the significance of surgery to outcome, and Cox proportional-hazards regression and competing risk model were conducted to determine the independent prognostic factors for these patients. A nomogram was built using multivariable logistic analyses to predict candidates for surgery based on preoperative factors.

Results

The final study population of 31,462 patients were divided into surgery and non-surgery groups. The median cancer-specific survival time respectively was 53 vs. 13 months. The patients’ age, sex, race, Tumor, Node, Metastasis score, stage, chemotherapy use, tumor histology and nuclear grade were independent prognostic factors. Apart from race and chemotherapy, other variates were included in the predictive model to distinguish the optimal surgical octogenarian candidates with NSCLC. Internal and external validation confirmed the efficacy of this model.

Conclusion

Surgery improved the survival time of octogenarian NSCLC patients. A novel nomogram was built to help clinicians make the decision to perform surgery on elderly patients with NSCLC.
Targeted sampling reduces the uncertainty in force of infection estimates from serological surveillance
Authors: Kim Kiyeon , Ito Kimihito
Published: July 2022

Age bins are frequently used in serological studies of infectious diseases in wildlife to deal with uncertainty in the age of sampled animals. This study analyzed how age binning and targeted sampling in serological surveillance affect the width of the 95% confidence interval (CI) of the estimated force of infection (FOI) of infectious diseases. We indicate that the optimal target population with the narrowest 95% CI differs depending on the expected FOI using computer simulations and mathematical models. In addition, our findings show that we can substantially reduce the number of animals required to infer transmission risk by tailoring targeted, age-based sampling to specific epidemiological situations.
Early feeding strategies in lambs affect rumen development and growth performance, with advantages persisting for two weeks after the transition to fattening diets
Authors: Liu Ting , Li Fadi , Wang Weimin , Wang Xiaojuan , Ma Zhiyuan , Li Chong , Weng Xiuxiu , Zheng Chen
Published: July 2022

This study aimed to explore the effects of early feeding strategies on the growth and rumen development of lambs from pre-weaning to the transition to fattening diets. Ninety-six newborn, male lambs with similar body weights were randomly assigned to three treatments: fed starter at 42 days old + weaned at 56 days old (Ctrl, n = 36), fed starter at 7 days old + weaned at 56 days old (ES, n = 36), and fed starter at 7 days old + weaned at 28 days old (ES + EW, n = 24). The fattening diets of all lambs were gradually replaced from 60 to 70 days of age. Six randomly selected lambs from each treatment were slaughtered at 14, 28, 42, 56, 70, and 84 days of age. The results showed that the richness and diversity of rumen microbiota of lambs in the Ctrl group were distinct from those of lambs in the other groups at 42 days of age. Moreover, transcriptome analysis revealed 407, 219, and 1,211 unique differentially expressed genes (DEGs) in the rumen tissue of ES vs. Ctrl, ES vs. ES + EW, and ES + EW vs. Ctrl groups, respectively, at 42 days of age. Different early feeding strategies resulted in differences in ruminal anatomy, morphology, and fermentation in lambs from 42 to 84 days of age (P < 0.05). Lambs in the ES + EW group had a higher average starter diet intake than those in the other groups (P < 0.05) from 28 to 56 days of age, which affected their growth performance. After 42 days of age, the body and carcass weights of lambs in the ES and ES + EW groups were higher than those in the Ctrl group (P < 0.05). These findings demonstrate that feeding lambs with a starter diet at 7 days of age and weaning them at 28 days of age can promote rumen development and improve growth performance, and this advantage persists for up to 2 weeks after transition to the fattening diet.
A polymorphism in porcine miR-22 is associated with pork color
Authors: Wang Han , Shen Zhonghao , Huang Ruihua , Zhao Ayong , Jiang Jiani , Li Pinghua , Zhou Xiaolong , Yang Songbai , Hou Liming
Published: July 2022

MicroRNAs (miRNAs) are posttranscriptional regulators that play key roles in meat color regulation. Changes in miRNA expression affect their target mRNAs, leading to multifunctional effects on biological processes and phenotypes. In this study, a G > A mutation site located upstream of the precursor miR-22 sequence in Suhuai pigs was significantly correlated with the meat color parameter a*(redness) of the porcine longissimus dorsi (LD) muscle. AA genotype individuals had the highest average meat color a* value and the lowest miR-22 level. When G > A mutation was performed in the miR-22 overexpression vector, miR-22 expression significantly decreased. Considering that Ca2+ homeostasis is closely related to pig meat color, our results further demonstrated that ELOVL6 is a direct target of miR-22 in pigs. The effects of miR-22 on skeletal muscle intracellular Ca2+ were partially caused by the suppression of ELOVL6 expression.
Effect of Eucommia ulmoides Leaf Extract on Growth Performance, Carcass Traits, Parameters of Oxidative Stress, and Lipid Metabolism in Broiler Chickens
Authors: Yan Jiahao , Hu Ruizhi , Li Baizhen , Tan Jijun , Wang Ying , Tang Zhiyi , Liu Ming , Fu Chenxing , He Jianhua , Wu Xiaosong
Published: July 2022

Eucommia ulmoides bark has been traditionally used as a Chinese medicine to attenuate stress, but the leaf, which is rich in polyphenols and polysaccharides, has been rarely used. This study aimed to investigate the effect of Eucommia ulmoides leaf extracts (EULEs) on oxidative stress and meat quality of broilers. A total of 252 broilers were randomly divided into 3 treatments and fed with a control basal diet (CON), or a diet containing 250 mg/kg or 1,000 mg/kg of EULE for 51 days. Results showed that dietary supplementation of 250 mg/kg EULE increased significantly the average daily gain of broilers in the early stage (1–21 days), while 250 mg/kg or 1,000 mg/kg of EULE decreased the feed conversion ratio in the whole period (P < 0.05). Supplementation of 250 mg/kg EULE reduced the level of MDA in the liver (P < 0.05), while 1,000 mg/kg EULE decreased the serum level of MDA (P < 0.05), and the HDL level in serum was increased by 250 mg/kg or 1,000 mg/kg EULE (P < 0.05). Additionally, 250 mg/kg EULE decreased abdominal fat ratio and serum triglyceride (TC) level in broilers, while 250 or 1,000 mg/kg of EULE reduced drip loss in breast muscle (P < 0.05), and 1,000 mg/kg EULE reduced the cooking loss in thigh muscle (P < 0.05). In conclusion, dietary supplementation of 250 mg/kg of EULE could attenuate oxidative stress and improve the growth performance and meat quality in broilers.
Archaea: An under-estimated kingdom in livestock animals
Authors: Peng Yunjuan , Xie Ting , Wu Zhuosui , Zheng Wenxiao , Zhang Tao , Howe Samantha , Chai Jianmin , Deng Feilong , Li Ying , Zhao Jiangchao
Published: July 2022

Archaea are considered an essential group of gut microorganisms in both humans and animals. However, they have been neglected in previous studies, especially those involving non-ruminants. In this study, we re-analyzed published metagenomic and metatranscriptomic data sequenced from matched samples to explore the composition and the expression activity of gut archaea in ruminants (cattle and sheep) and monogastric animals (pig and chicken). Our results showed that the alpha and beta diversity of each host species, especially cattle and chickens, calculated from metagenomic and metatranscriptomic data were significantly different, suggesting that metatranscriptomic data better represent the functional status of archaea. We detected that the relative abundance of 17 (cattle), 7 (sheep), 20 (pig), and 2 (chicken) archaeal species were identified in the top 100 archaeal taxa when analyzing the metagenomic datasets, and these species were classified as the “active archaeal species” for each host species by comparison with corresponding metatranscriptomic data. For example, The expressive abundance in metatranscriptomic dataset of Methanosphaera cuniculi and Methanosphaera stadtmanae were 30- and 27-fold higher than that in metagenomic abundance, indicating their potentially important function in the pig gut. Here we aim to show the potential importance of archaea in the livestock digestive tract and encourage future research in this area, especially on the gut archaea of monogastric animals.
Optimization of Compensation Network for a Wireless Power Transfer System in Dynamic Conditions: A Circuit Analysis Approach
Authors: Bertoluzzo Manuele , Di Barba Paolo , Forzan Michele , Mognaschi Maria Evelina , Sieni Elisabetta
Published: July 2022

The paper is focused on the optimization of the compensation network of a wireless power transfer system (WPTS) intended to operate in dynamic conditions. A laboratory prototype of a WPTS has been taken as a reference in this work, allowing for the experimental data and all the numerical models here presented to reproduce the configuration of the existing device. The numerical model has been used to perform FEM analysis with variable relative positions of the emitting and receiving coil to simulate the movement in a ‘recharge while driving’ condition. Inductive lumped parameters, i.e., self and mutual inductances computed from FEM results, have been used for the optimal design of the compensation network necessary for the WPTS operation. The optimal design of the resonance circuits has been developed by defining objective functions, aiming to achieve these goals: transmitted power must be as constant as possible when the vehicle is in movement and the electrical efficiency must be satisfactory high in most of the coupling conditions. The performances of the optimized network are finally compared and discussed.
Fortification of Pectin/Blackberry Hydrogels with Apple Fibers: Effect on Phenolics, Antioxidant Activity and Inhibition of α-Glucosidase
Authors: Kopjar Mirela , Ćorković Ina , Buljeta Ivana , Šimunović Josip , Pichler Anita
Published: July 2022

The objective of this study was to prepare hydrogels based on pectin and blackberry juice and additionally to fortify those hydrogels with apple fiber. For that purpose, two types of pectin (low methoxylated and high methoxylated) were used, and fortification was conducted with the addition of 10% of apple fiber. The hydrogels were evaluated for phenolic compounds, antioxidant activity and inhibition of α-glucosidase. In addition, the stability of these parameters after 8 months of storage was evaluated. Pectin type and addition of apple fiber had an impact on investigated parameters. Low methoxylated pectin hydrogels had a higher concentration of anthocyanins than high methoxylated pectin hydrogels, while the addition of apple fibers caused a decrease in anthocyanin content. However, fortified hydrogels had higher antioxidant activity due to the presence of phenolics from apple fibers. The results showed that anthocyanins were more favorable in inhibiting α-glucosidase because samples with higher anthocyanins concentration had lower IC50 values. Obtained hydrogels can be used as intermediate products or ingredients (like fruit fillings or spreads) for the improvement or development of novel food products to increase their fiber content and antioxidant potential.
Unraveling the Effects of Carotenoids Accumulation in Human Papillary Thyroid Carcinoma
Authors: di Masi Alessandra , Sessa Rosario Luigi , Cerrato Ylenia , Pastore Gianni , Guantario Barbara , Ambra Roberto , Di Gioacchino Michael , Sodo Armida , Verri Martina , Crucitti Pierfilippo , Longo Filippo , Naciu Anda Mihaela , Palermo Andrea , Taffon Chiara , Acconcia Filippo , Bianchi Fabrizio , Ascenzi Paolo , Ricci Maria Antonietta , Crescenzi Anna
Published: July 2022

Among the thyroid cancers, papillary thyroid cancer (PTC) accounts for 90% of the cases. In addition to the necessity to identify new targets for PTC treatment, early diagnosis and management are highly demanded. Previous data indicated that the multivariate statistical analysis of the Raman spectra allows the discrimination of healthy tissues from PTC ones; this is characterized by bands typical of carotenoids. Here, we dissected the molecular effects of carotenoid accumulation in PTC patients by analyzing whether they were required to provide increased retinoic acid (RA) synthesis and signaling and/or to sustain antioxidant functions. HPLC analysis revealed the lack of a significant difference in the overall content of carotenoids. For this reason, we wondered whether the carotenoid accumulation in PTC patients could be related to vitamin A derivative retinoic acid (RA) biosynthesis and, consequently, the RA-related pathway activation. The transcriptomic analysis performed using a dedicated PCR array revealed a significant downregulation of RA-related pathways in PTCs, suggesting that the carotenoid accumulation in PTC could be related to a lower metabolic conversion into RA compared to that of healthy tissues. In addition, the gene expression profile of 474 PTC cases previously published in the framework of the Cancer Genome Atlas (TGCA) project was examined by hierarchical clustering and heatmap analyses. This metanalysis study indicated that the RA-related pathways resulted in being significantly downregulated in PTCs and being associated with the follicular variant of PTC (FV-PTC). To assess whether the possible fate of the carotenoids accumulated in PTCs is associated with the oxidative stress response, the expression of enzymes involved in ROS scavenging was checked. An increased oxidative stress status and a reduced antioxidant defense response were observed in PTCs compared to matched healthy thyroids; this was possibly associated with the prooxidant effects of high levels of carotenoids. Finally, the DepMap datasets were used to profile the levels of 225 metabolites in 12 thyroid cancer cell lines. The results obtained suggested that the high carotenoid content in PTCs correlates with tryptophan metabolism. This pilot provided novel possible markers and possible therapeutic targets for PTC diagnosis and therapy. For the future, a larger study including a higher number of PTC patients will be necessary to further validate the molecular data reported here.
High Exogenous Antioxidant, Restorative Treatment (Heart) for Prevention of the Six Stages of Heart Failure: The Heart Diet
Authors: Singh Ram B. , Fedacko Jan , Pella Dominik , Fatima Ghizal , Elkilany Galal , Moshiri Mahmood , Hristova Krasimira , Jakabcin Patrik , Vaňova Natalia
Published: July 2022

The exact pathophysiology of heart failure (HF) is not yet known. Western diet, characterized by highly sweetened foods, as well as being rich in fat, fried foods, red meat and processed meat, eggs, and sweet beverages, may cause inflammation, leading to oxidative dysfunction in the cardiac ultra-structure. Oxidative function of the myocardium and how oxidative dysfunction causes physio-pathological remodeling, leading to HF, is not well known. Antioxidants, such as polyphenolics and flavonoids, omega-3 fatty acids, and other micronutrients that are rich in Indo-Mediterranean-type diets, could be protective in sustaining the oxidative functions of the heart. The cardiomyocytes use glucose and fatty acids for the physiological functions depending upon the metabolic requirements of the heart. Apart from toxicity due to glucose, lipotoxicity also adversely affects the cardiomyocytes, which worsen in the presence of deficiency of endogenous antioxidants and deficiency of exogenous antioxidant nutrients in the diet. The high-sugar-and-high-fat-induced production of ceramide, advanced glycation end products (AGE) and triamino-methyl-N-oxide (TMAO) can predispose individuals to oxidative dysfunction and Ca-overloading. The alteration in the biology may start with normal cardiac cell remodeling to biological remodeling due to inflammation. An increase in the fat content of a diet in combination with inducible nitric oxide synthase (NOSi) via N-arginine methyl ester has been found to preserve the ejection fraction in HF. It is proposed that a greater intake of high exogenous antioxidant restorative treatment (HEART) diet, polyphenolics and flavonoids, as well as cessation of red meat intake and egg, can cause improvement in the oxidative function of the heart, by inhibiting oxidative damage to lipids, proteins and DNA in the cell, resulting in beneficial effects in the early stage of the Six Stages of HF. There is an unmet need to conduct cohort studies and randomized, controlled studies to demonstrate the role of the HEART diet in the treatment of HF.
Hydroxytyrosol Recovers SARS-CoV-2-PLpro-Dependent Impairment of Interferon Related Genes in Polarized Human Airway, Intestinal and Liver Epithelial Cells
Authors: Crudele Annalisa , Smeriglio Antonella , Ingegneri Mariarosaria , Panera Nadia , Bianchi Marzia , Braghini Maria Rita , Pastore Anna , Tocco Valeria , Carsetti Rita , Zaffina Salvatore , Alisi Anna , Trombetta Domenico
Published: July 2022

The SARS-CoV-2 pandemic has caused approximately 6.3 million deaths, mainly due to the acute respiratory distress syndrome or multi-organ failure that characterizes COVID-19 acute disease. Post-acute COVID-19 syndrome, also known as long-COVID, is a condition characterized by a complex of symptoms that affects 10–20% of the individuals who have recovered from the infection. Scientific and clinical evidence demonstrates that long-COVID can develop in both adults and children. It has been hypothesized that multi-organ effects of long-COVID could be associated with the persistence of virus RNA/proteins in host cells, but the real mechanism remains to be elucidated. Therefore, we sought to determine the effects of the exogenous expression of the papain-like protease (PLpro) domain of the non-structural protein (NSP3) of SARS-CoV-2 in polarized human airway (Calu-3), intestinal (Caco-2), and liver (HepG2) epithelial cells, and to evaluate the ability of the natural antioxidant hydroxytyrosol (HXT) in neutralizing these effects. Our results demonstrated that PLpro was able to induce a cascade of inflammatory genes and proteins (mainly associated with the interferon pathway) and increase the apoptotic rate and expression of several oxidative stress markers in all evaluated epithelial cells. Noteably, the treatment with 10 μM HXT reverted PL-pro-dependent effects almost completely. This study provides the first evidence that SARS-CoV-2 PLpro remaining in host cells after viral clearance may contribute to the pathogenetic mechanisms of long-COVID. These effects may be counteracted by natural antioxidants. Further clinical and experimental studies are necessary to confirm this hypothesis.
BAG9 Confers Thermotolerance by Regulating Cellular Redox Homeostasis and the Stability of Heat Shock Proteins in Solanum lycopersicum
Authors: Huang Huamin , Liu Chenxu , Yang Chen , Kanwar Mukesh Kumar , Shao Shujun , Qi Zhenyu , Zhou Jie
Published: July 2022

The Bcl-2-associated athanogene (BAG) family, a group of co-chaperones that share conservative domains in flora and fauna, is involved in plant growth, development, and stress tolerance. However, the function of tomato BAG genes on thermotolerance remains largely unknown. Herein, we found that the expression of BAG9 was induced during heat stress in tomato plants. Knockout of the BAG9 gene by CRISPR/Cas9 reduced, while its overexpression increased thermotolerance in tomato plants as reflected by the phenotype, photosynthesis rate, and membrane peroxidation. Heat-induced reactive oxygen species and oxidative/oxidized proteins were further increased in bag9 mutants and were normalized in BAG9 overexpressing plants. Furthermore, the activities of antioxidant enzymes, ascorbic acid (AsA)/dehydroascorbic acid (DHA), and reduced glutathione (GSH)/oxidized glutathione (GSSG) were reduced in bag9 mutants and were increased in BAG9 overexpressing plants under heat stress. Additionally, BAG9 interacted with Hsp20 proteins in vitro and in vivo. Accumulation of Hsp proteins induced by heat showed a reduction in bag9 mutants; meanwhile, it was increased in BAG9 overexpressing plants. Thus, BAG9 played a crucial role in response to heat stress by regulating cellular redox homeostasis and the stability of heat shock proteins.
Overexpression of Heat Shock Protein 70 Ameliorates Meat Quality of Broilers Subjected to Pre-Slaughter Transport at High Ambient Temperatures by Improving Energy Status of Pectoralis Major Muscle and Antioxidant Capacity
Authors: Xing Tong , Xu Xinglian , Zhang Lin , Gao Feng
Published: July 2022

The induction of heat shock protein 70 (HSP70) potentially mediates meat-quality development under stress conditions. To investigate the effects and mechanism of HSP70 on the meat quality of the pectoralis major (PM) muscles of broilers exposed to pre-slaughter transport, a total of 168 broilers were intraperitoneally injected with L-glutamine (Gln) or saline. Twenty-four hours later, broilers were subjected to transport or held under normal living conditions. The results indicated that acute Gln supplementation significantly increased HSP70 expression in the PM of transported broilers (p < 0.05). The overexpression of HSP70 significantly alleviated the decreases in muscle pH and water-holding capacity and improved the shrinking of muscle fibers induced by transport (p < 0.05). HSP70 induction increased ATP content, decreased the activities of glycolytic enzymes, and lowered the phosphorylation level of AMP-activated protein kinase in transported broilers (p < 0.05). In addition, the overexpression of HSP70 greatly increased total superoxide dismutase and the total antioxidant capability and decreased the levels of reactive oxygen species, malonaldehyde, and carbonyls in the PM of transported broilers (p < 0.05). Overall, this work indicated that HSP70 could effectively improve the meat quality of transported broilers by improving the energy status, inhibiting glycolytic influx, and restoring redox homeostasis.
Mutant Huntingtin Derails Cysteine Metabolism in Huntington’s Disease at Both Transcriptional and Post-Translational Levels
Authors: Paul Bindu D. , Sbodio Juan I. , Snyder Solomon H.
Published: July 2022

Cysteine is a semi-essential amino acid that not only plays an essential role as a component of protein synthesis, but also in the generation of numerous sulfur-containing molecules such as the antioxidant glutathione and coenzyme A. We previously showed that the metabolism of cysteine is dysregulated in Huntington’s disease (HD), a neurodegenerative disorder triggered by the expansion of polyglutamine repeats in the protein huntingtin. In this study, we showed that cysteine metabolism is compromised at multiple levels in HD, both transcriptional and post-translational. Accordingly, restoring cysteine homeostasis may be beneficial in HD.
Influence of Neonatal Sex on Breast Milk Protein and Antioxidant Content in Spanish Women in the First Month of Lactation
Authors: Ramiro-Cortijo David , Gila-Diaz Andrea , Herranz Carrillo Gloria , Cañas Silvia , Gil-Ramírez Alicia , Ruvira Santiago , Martin-Cabrejas María A. , Arribas Silvia M.
Published: July 2022

Breast milk (BM) is the best food for newborns. Male sex is associated with a higher risk of fetal programming, prematurity, and adverse postnatal outcome, being that BM is an important health determinant. BM composition is dynamic and modified by several factors, including lactation period, prematurity, maternal nutritional status, and others. This study was designed to evaluate the influence of sex on BM composition during the first month of lactation, focused on macronutrients and antioxidants. Forty-eight breastfeeding women and their fifty-five newborns were recruited at the Hospital Clínico San Carlos (Madrid, Spain). Clinical sociodemographic data and anthropometric parameters were collected. BM samples were obtained at days 7, 14, and 28 of lactation to assess fat (Mojonnier method), protein (Bradford method), and biomarkers of oxidative status: total antioxidant capacity (ABTS and FRAP methods), thiol groups, reduced glutathione, superoxide dismutase and catalase activities, lipid peroxidation, and protein oxidation (spectrophotometric methods). Linear mixed models with random effects adjusted by maternal anthropometry, neonatal Z-scores at birth, and gestational age were used to assess the main effects of sex, lactation period, and their interaction. BM from mothers with male neonates exhibited significantly higher protein, ABTS, FRAP, and GSH levels, while catalase showed the opposite trend. No differences between sexes were observed in SOD, total thiols, and oxidative damage biomarkers. Most changes were observed on day 7 of lactation. Adjusted models demonstrated a significant association between male sex and proteins (β = 2.70 ± 1.20; p-Value = 0.048). In addition, total antioxidant capacity by ABTS (β = 0.11 ± 0.06) and GSH (β = 1.82 ± 0.94) showed a positive trend near significance (p-Value = 0.056; p-Value = 0.064, respectively). In conclusion, transitional milk showed sex differences in composition with higher protein and GSH levels in males. This may represent an advantage in the immediate perinatal period, which may help to counteract the worse adaptation of males to adverse intrauterine environments and prematurity.
Gearbox Fault Diagnosis Based on Multi-Sensor and Multi-Channel Decision-Level Fusion Based on SDP
Authors: Fu Yuan , Chen Xiang , Liu Yu , Son Chan , Yang Yan
Published: July 2022

In order to deal with the shortcomings (such as poor robustness) of the traditional single-channel vibration signal in the comprehensive monitoring of the gearbox fault state, a multi-channel decision-level fusion algorithm was proposed based on symmetrized dot pattern (SDP) analysis, with the visual geometry group 16 network (VGG16) fault diagnosis model. Firstly, the SDP method was used to convert the vibration signal of a single multi-channel sensor into an imaging arm. Secondly, the obtained image arm was input into the VGG16 convolutional neural network in order to train the fault diagnosis model that can be obtained. Then, the SDP images of the signals that were to be measured from multiple multi-channel sensors were input into the fault diagnosis model, and the diagnosis results of multiple multi-channel sensors could then be obtained. Experimentally, it was demonstrated that the diagnostic results of multi-channel sensors one, two, and three were more accurate than those of single-channel sensors one, two, and three, by 3.01%, 16.7%, and 5.17%, respectively. However, the fault generation was not generated in a single direction, but rather multiple directions. In order to improve the comprehensiveness of the raw vibration data, a fusion method using DS (Dempster–Shafer) evidence theory was proposed in order to fuse multiple multi-channel sensors, in which the accuracy achieved 99.93% when sensor one and sensor two were fused, which was an improvement of 8.88% and 1.02% over single sensors one and two, respectively. When sensor one and sensor three were fused, the accuracy reached 99.31%, which was an improvement of 8.31% and 6.17% over single sensors one and three, respectively. When sensor two and sensor three were fused, the accuracy reached 99.91%, which was an improvement of 1.00% and 6.74% over single sensors two and three, respectively. When three sensors were fused simultaneously, the accuracy reached 99.99%, which was 8.93%, 1.08%, and 6.81% better than single sensors one, two, and three, respectively. Therefore, it can be proved that the number of sensor channels has a great influence on the diagnosis results.
Discriminating Pattern Mining for Diagnosing Reading Disorders
Authors: Fassetti Fabio , Fassetti Ilaria
Published: July 2022

Tachistoscopes are devices that display a word for several seconds and ask the user to write down the word. They have been widely employed to increase recognition speed, to increase reading comprehension and, especially, to individuate reading difficulties and disabilities. Once the therapist is provided with the answers of the patients, a challenging problem is the analysis of the strings to individuate common patterns in the erroneous strings that could raise suspicion of related disabilities. In this direction, this work presents a machine learning technique aimed at mining exceptional string patterns and is precisely designed to tackle the above-mentioned problem. The technique is based on non-negative matrix factorization, nnmf, and exploits as features the structure of the words in terms of the letters composing them. To the best of our knowledge, this is the first attempt of mining tachistoscope answers to discover intrinsic peculiarities of the words possibly involved in reading disabilities. From the technical point of view, we present a novel variant of nnmf methods with the adjunctive goal of discriminating between sets. The technique has been experimented in a real case study with the help of an Italian speech therapist center that collaborate with this work.