Chemical identification of fossil filament entrapped in Messinian gypsum using space Laser Mass Spectrometry, application for Mars astrobiology.
Authors: Sellam Youcef , Gruchola Salome , Reghizzi Matteo , Lugli Stefano , Riedo Andreas , Wurz Peter 
Published: February 2023

The search for evidence of extant and extinct life on Mars is of big interest for future robotic and human exploration missions. The properties and characteristics of evaporites such as gypsum make them an easily accessible target in the search for biosignatures of extraterrestrial life. On Earth, a diverse microbial community inhabits gypsum in modern brines. These microbes are easily entombed within gypsum due to its fast precipitation. Studies of primary lower gypsum deposits, accumulated in the Mediterranean Basin during the Messinian salinity crisis (5.97 &#8211; 5.33 Ma), revealed a well-preserved and prominent archive of diverse microbial life. Similar archives might exist on Mars considering the desiccation of large bodies of water there, representing a promising target for exploring the possibility of life on Mars. The recent imaging mapping and analysis done by orbiters and rovers on the surface of Mars led to the discovery of abundant gypsum and evaporite minerals suggesting the evaporation of large lakes and lacustrine systems and as the surface of Mars dried out, hypersaline lakes would have filled the ancient lake system. A similar scenario happened on Earth when thick layers of gypsum were deposited during the Messinian salinity crisis when the Mediterranean was turned into the youngest salt giant in Earth&#8217;s history.Previous chemical and molecular laser mass spectrometry analyses done on microfossils preserved in different rock records showed these instruments&#8217; ability to detect biosignatures related to ancient life. In this study, we successfully investigated the chemical composition of fossil filaments interpreted to be benthic microbial assemblages dominated by chemotrophic sulfide-oxidizing bacteria, sulfate-reducing bacteria, and planktonic cyanobacteria. This study demonstrated the efficiency of a miniaturized next-generation Laser based Mass Spectrometer (LMS) designed for future space explorations in the detection of microbial chemical biosignatures trapped in Messinian gypsum, shading light to the potential application to the search for life on Mars.&#160;&#160;&#160;&#160;Literature&#160;Natalicchio, M., Birgel, D., Dela Pierre, F., Ziegenbalg, S., Hoffmann-Sell, L., Gier, S., & Peckmann, J. (2022). Messinian bottom-grown selenitic gypsum: An archive of microbial life. Geobiology, 20, 3&#8211; 21. https://doi.org/10.1111/gbi.12464.Bayles, M., Belasco, B.C., Breda, A.J., Cahill, C.B., Da Silva, A.Z., Regan, M.J., Jr., Schlamp, N.K., Slagle, M.P. and Baxter, B.K. (2020). The Haloarchaea of Great Salt Lake as Models for Potential Extant Life on Mars. In Extremophiles as Astrobiological Models (eds J. Seckbach and H. Stan-Lotter). https://doi.org/10.1002/9781119593096.ch4.Lukmanov, RA., Tulej, M., Ligterink, NFW., et al. ( 2021). Chemical identification of microfossils from the 1.88-Ga Gunflint chert: Towards empirical biosignatures using laser ablation ionization mass spectrometer. Journal of Chemometrics, 35 ( 10):e3370. doi:10.1002/cem.3370.