bluuebunny/crossref_metadata_2025 huggingface dataset:
CT Findings and Histological Evaluation of Red Foxes (Vulpes vulpes) with Chronic Head Trauma Injury: A Retrospective Study
Authors: Lacitignola Luca , Samarelli Rossella , Zizzo Nicola , Circella Elena , Acquafredda Claudia , Stabile Marzia , Lombardi Roberto , Staffieri Francesco , Camarda Antonio
Published: April 2021

Large numbers of wild animals are injured every year in road traffic accidents. Scant data are available for rescued wild carnivores, in particular for red foxes. Cases of foxes with head trauma were retrospectively considered for inclusion in this study. Clinical examination, modified Glasgow coma scale (MGCS), computed tomography (CT) examination, therapy, outcome, and post mortem findings of the brain were investigated. In all foxes, cranial vaults lesion occurred in single (67%) or multiple sites (33%). Midline shift and hydrocephalus were observed in this population. The mean survival was 290 (±176) days. In our study, we performed CT scans on average 260 days after fox rescue, and we speculate that persisting clinical signs could be attributed to TBI. In our study, only two foxes were alive at the time of writing. Other foxes were euthanized due to the severity of the clinical signs. CT scans help diagnose chronic lesions and their effect on prognostic judgment for animals released to wildlife environments.
Can Vitamin B12 Assist the Internalization of Antisense LNA Oligonucleotides into Bacteria?
Authors: Pereira Sara , Yao Ruwei , Gomes Mariana , Jørgensen Per Trolle , Wengel Jesper , Azevedo Nuno Filipe , Sobral Santos Rita
Published: April 2021

The emergence of bacterial resistance to traditional small-molecule antibiotics is fueling the search for innovative strategies to treat infections. Inhibiting the expression of essential bacterial genes using antisense oligonucleotides (ASOs), particularly composed of nucleic acid mimics (NAMs), has emerged as a promising strategy. However, their efficiency depends on their association with vectors that can translocate the bacterial envelope. Vitamin B12 is among the largest molecules known to be taken up by bacteria and has very recently started to gain interest as a trojan-horse vector. Gapmers and steric blockers were evaluated as ASOs against Escherichia coli (E. coli). Both ASOs were successfully conjugated to B12 by copper-free azide-alkyne click-chemistry. The biological effect of the two conjugates was evaluated together with their intracellular localization in E. coli. Although not only B12 but also both B12-ASO conjugates interacted strongly with E. coli, they were mostly colocalized with the outer membrane. Only 6–9% were detected in the cytosol, which showed to be insufficient for bacterial growth inhibition. These results suggest that the internalization of B12-ASO conjugates is strongly affected by the low uptake rate of the B12 in E. coli and that further studies are needed before considering this strategy against biofilms in vivo.
Antibiotic Resistance and Genotypes of Nosocomial Strains of Acinetobacter baumannii in Kazakhstan
Authors: Lavrinenko Alyona , Sheck Eugene , Kolesnichenko Svetlana , Azizov Ilya , Turmukhambetova Anar
Published: April 2021

The aim of this study was to determine the prevalence of A. baumannii antibiotic-resistant strains in Kazakhstan and to characterize genotypes related to epidemic “high-risk” clones. Two hundred and twenty four A. baumannii isolates from four cities of Kazakhstan in 2011–2019 were studied. Antibiotic susceptibility testing was performed by using broth microdilutions method according to EUCAST (v 11.0) recommendations. The presence of blaOXA-23-like, blaOXA-24/40-like,blaOXA-58-like,blaVIM,blaIMP, and blaNDM genes was determined by PCR. Genotyping was performed using high-throughput real-time PCR detection of 21 SNPs at 10 chromosomal loci used in existing MLST schemes. Resistance rates to imipenem, meropenem, amikacin, gentamicin, and ciprofloxacin were 81.3%, 78.6%, 79.9%, 65.2%, and 89.3%, respectively. No colistin resistant isolates were detected. The values of the MIC 50% and the MIC 90% of tigecycline were 0.125 mg/L, only four isolates (1.8%) had the ECOFF value >0.5 mg/L. The presence of acquired carbapenemase genes was found in 82.2% strains, including blaOXA-23-like (78.6%) or blaOXA-58-like (3.6%) genes. The spreading of carbapenem resistant A. baumannii strains in Kazakhstan was associated with epidemic “high-risk” clonal groups, predominantly, CG208(92)OXF/CG2PAS (80.8%) and less often CG231(109)OXF/CG1PAS (1.8%).
Finding What Is Inaccessible: Antimicrobial Resistance Language Use among the One Health Domains
Authors: Wind Lauren L. , Briganti Jonathan S. , Brown Anne M. , Neher Timothy P. , Davis Meghan F. , Durso Lisa M. , Spicer Tanner , Lansing Stephanie
Published: April 2021

The success of a One Health approach to combating antimicrobial resistance (AMR) requires effective data sharing across the three One Health domains (human, animal, and environment). To investigate if there are differences in language use across the One Health domains, we examined the peer-reviewed literature using a combination of text data mining and natural language processing techniques on 20,000 open-access articles related to AMR and One Health. Evaluating AMR key term frequency from the European PubMed Collection published between 1990 and 2019 showed distinct AMR language usage within each domain and incongruent language usage across domains, with significant differences in key term usage frequencies when articles were grouped by the One Health sub-specialties (2-way ANOVA; p < 0.001). Over the 29-year period, “antibiotic resistance” and “AR” were used 18 times more than “antimicrobial resistance” and “AMR”. The discord of language use across One Health potentially weakens the effectiveness of interdisciplinary research by creating accessibility issues for researchers using search engines. This research was the first to quantify this disparate language use within One Health, which inhibits collaboration and crosstalk between domains. We suggest the following for authors publishing AMR-related research within the One Health context: (1) increase title/abstract searchability by including both antimicrobial and antibiotic resistance related search terms; (2) include “One Health” in the title/abstract; and (3) prioritize open-access publication.
Potential Roles for Probiotics in the Treatment of COVID-19 Patients and Prevention of Complications Associated with Increased Antibiotic Use
Authors: Kullar Ravina , Johnson Stuart , McFarland Lynne V. , Goldstein Ellie J. C.
Published: April 2021

Medical care for patients hospitalized with COVID-19 is an evolving process. Most COVID-19 inpatients (58–95%) received empiric antibiotics to prevent the increased mortality due to ventilator-associated pneumonia and other secondary infections observed in COVID-19 patients. The expected consequences of increased antibiotic use include antibiotic-associated diarrhea (AAD) and Clostridioides difficile infections (CDI). We reviewed the literature (January 2020–March 2021) to explore strategies to reduce these consequences. Antimicrobial stewardship programs were effective in controlling antibiotic use during past influenza epidemics and have also been shown to reduce healthcare-associated rates of CDI. Another potential strategy is the use of specific strains of probiotics shown to be effective for the prevention of AAD and CDI prior to the pandemic. During 2020, there was a paucity of published trials using these two strategies in COVID-19 patients, but trials are currently ongoing. A multi-strain probiotic mixture was found to be effective in reducing COVID-19-associated diarrhea in one trial. These strategies are promising but need further evidence from trials in COVID-19 patients.
Sub-Inhibitory Concentrations of Ciprofloxacin Alone and Combinations with Plant-Derived Compounds against P. aeruginosa Biofilms and Their Effects on the Metabolomic Profile of P. aeruginosa Biofilms
Authors: Kart Didem , Reçber Tuba , Nemutlu Emirhan , Sagiroglu Meral
Published: April 2021

Introduction: Alternative anti-biofilm agents are needed to combat Pseudomonas aeruginosa infections. The mechanisms behind these new agents also need to be revealed at a molecular level. Materials and methods: The anti-biofilm effects of 10 plant-derived compounds on P. aeruginosa biofilms were investigated using minimum biofilm eradication concentration (MBEC) and virulence assays. The effects of ciprofloxacin and compound combinations on P. aeruginosa in mono and triple biofilms were compared. A metabolomic approach and qRT-PCR were applied to the biofilms treated with ciprofloxacin in combination with baicalein, esculin hydrate, curcumin, and cinnamaldehyde at sub-minimal biofilm inhibitory concentration (MBIC) concentrations to highlight the specific metabolic shifts between the biofilms and to determine the quorum sensing gene expressions, respectively. Results: The combinations of ciprofloxacin with curcumin, baicalein, esculetin, and cinnamaldehyde showed more reduced MBICs than ciprofloxacin alone. The quorum sensing genes were downregulated in the presence of curcumin and cinnamaldehyde, while upregulated in the presence of baicalein and esculin hydrate rather than for ciprofloxacin alone. The combinations exhibited different killing effects on P. aeruginosa in mono and triple biofilms without affecting its virulence. The findings of the decreased metabolite levels related to pyrimidine and lipopolysaccharide synthesis and to down-regulated alginate and lasI expressions strongly indicate the role of multifactorial mechanisms for curcumin-mediated P. aeruginosa growth inhibition. Conclusions: The use of curcumin, baicalein, esculetin, and cinnamaldehyde with ciprofloxacin will help fight against P. aeruginosa biofilms. To the best of our knowledge, this is the first study of its kind to define the effect of plant-based compounds as possible anti-biofilm agents with low MBICs for the treatment of P. aeruginosa biofilms through metabolomic pathways.
Determination of Suitable Macroporous Resins and Desorbents for Carnosol and Carnosic Acid from Deep Eutectic Solvent Sage (Salvia officinalis) Extract with Assessment of Antiradical and Antibacterial Activity
Authors: Jakovljević Kovač Martina , Pavić Valentina , Huđ Anastazija , Cindrić Ines , Molnar Maja
Published: April 2021

In this study, for the first time, the adsorption/desorption characteristics of carnosic acid and carnosol from deep eutectic solvent extract of Salvia officinalis on five macroporous resins (HP20, XAD7HP, XAD16N, HP21, HP2MG) were evaluated. The high adsorption and medium desorption capacities of carnosic acid and carnosol as well as antibacterial and antiradical activity from the extract obtained with choline chloride:lactic acid (1:2) on XAD7HP resin indicated that resin was appropriate. To get the optimal separation process, the influence of factors such as adsorption/desorption time and volume of desorbent was further investigated. The results showed that the extract with high antiradical and antibacterial activity was obtained via adsorption and desorption on XAD7HP resin. The extraction efficiencies of the deep eutectic solvents (DESs) recycled once, twice, and thrice were 97.64% (±0.03%), 93.10% (±0.66%), and 88.94% (±1.15%), respectively, for carnosic acid, and 96.63% (±0.04%), 94.38% (±0.27%), and 91.19% (±0.36%), respectively, for carnosol, relative to the initial solvent efficiency. Based on that, this method is a promising basis for the large-scale preparation of extracts from Salvia officinalis with further application in the pharmaceutical or food industry, especially for maintaining the “green” character of the whole process to obtain the appropriate extract.
Cisd2 Protects the Liver from Oxidative Stress and Ameliorates Western Diet-Induced Nonalcoholic Fatty Liver Disease
Authors: Huang Yi-Long , Shen Zhao-Qing , Huang Chen-Hua , Teng Yuan-Chi , Lin Chao-Hsiung , Tsai Ting-Fen
Published: April 2021

Nonalcoholic fatty liver disease (NAFLD) and its more severe form, nonalcoholic steatohepatitis (NASH), are the most common chronic liver diseases worldwide. However, drugs to treat NAFLD and NASH are an unmet clinical need. This study sought to provide evidence that Cisd2 is a molecular target for the development of treatments targeting NAFLD and NASH. Several discoveries are pinpointed. The first is that Cisd2 dosage modulates the severity of Western diet-induced (WD-induced) NAFLD. Specifically, Cisd2 haploinsufficiency accelerates NAFLD development and exacerbates progression toward NASH. Conversely, an enhanced Cisd2 copy number attenuates liver pathogenesis. Secondly, when a WD is fed to mice, transcriptomic analysis reveals that the major alterations affecting biological processes are related to inflammation, lipid metabolism, and DNA replication/repair. Thirdly, among these differentially expressed genes, the most significant changes involve Nrf2-mediated oxidative stress, cholesterol biosynthesis, and fatty acid metabolism. Finally, increased Cisd2 expression protects the liver from oxidative stress and reduces the occurrence of mitochondrial DNA deletions. Taken together, our mouse model reveals that Cisd2 plays a crucial role in protecting the liver from WD-induced damages. The development of therapeutic agents that effectively enhance Cisd2 expression is one potential approach to the treatment of WD-induced fatty liver diseases.
Portable near Infrared Spectroscopy as a Tool for Fresh Tomato Quality Control Analysis in the Field
Authors: Borba Karla R. , Aykas Didem P. , Milani Maria I. , Colnago Luiz A. , Ferreira Marcos D. , Rodriguez-Saona Luis E.
Published: April 2021

Portable spectrometers are promising tools that can be an alternative way, for various purposes, of analyzing food quality, such as monitoring in a few seconds the internal quality during fruit ripening in the field. A portable/handheld (palm-sized) near-infrared (NIR) spectrometer (Neospectra, Si-ware) with spectral range of 1295–2611 nm, equipped with a micro-electro-mechanical system (MEMs), was used to develop prediction models to evaluate tomato quality attributes non-destructively. Soluble solid content (SSC), fructose, glucose, titratable acidity (TA), ascorbic, and citric acid contents of different types of fresh tomatoes were analyzed with standard methods, and those values were correlated to spectral data by partial least squares regression (PLSR). Fresh tomato samples were obtained in 2018 and 2019 crops in commercial production, and four fruit types were evaluated: Roma, round, grape, and cherry tomatoes. The large variation in tomato types and having the fruits from distinct years resulted in a wide range in quality parameters enabling robust PLSR models. Results showed accurate prediction and good correlation (Rpred) for SSC = 0.87, glucose = 0.83, fructose = 0.87, ascorbic acid = 0.81, and citric acid = 0.86. Our results support the assertion that a handheld NIR spectrometer has a high potential to simultaneously determine several quality attributes of different types of tomatoes in a practical and fast way.
Enzymatic Synthesis and Characterization of Different Families of Chitooligosaccharides and Their Bioactive Properties
Authors: Miguez Noa , Kidibule Peter , Santos-Moriano Paloma , Ballesteros Antonio O. , Fernandez-Lobato Maria , Plou Francisco J.
Published: April 2021

Chitooligosaccharides (COS) are homo- or hetero-oligomers of D-glucosamine (GlcN) and N-acetyl-D-glucosamine (GlcNAc) that can be obtained by chitosan or chitin hydrolysis. Their enzymatic production is preferred over other methodologies (physical, chemical, etc.) due to the mild conditions required, the fewer amounts of waste and its efficiency to control product composition. By properly selecting the enzyme (chitinase, chitosanase or nonspecific enzymes) and the substrate properties (degree of deacetylation, molecular weight, etc.), it is possible to direct the synthesis towards any of the three COS types: fully acetylated (faCOS), partially acetylated (paCOS) and fully deacetylated (fdCOS). In this article, we review the main strategies to steer the COS production towards a specific group. The chemical characterization of COS by advanced techniques, e.g., high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and MALDI-TOF mass spectrometry, is critical for structure–function studies. The scaling of processes to synthesize specific COS mixtures is difficult due to the low solubility of chitin/chitosan, the heterogeneity of the reaction mixtures, and high amounts of salts. Enzyme immobilization can help to minimize such hurdles. The main bioactive properties of COS are herein reviewed. Finally, the anti-inflammatory activity of three COS mixtures was assayed in murine macrophages after stimulation with lipopolysaccharides.
Parametric Analysis of a Polygeneration System with CO2 Working Fluid
Authors: Bellos Evangelos , Tzivanidis Christos
Published: April 2021

The objective of the present work is the investigation of a novel polygeneration system for power, refrigeration and heating production at two temperature levels. The present system uses CO2 as the working fluid, which is an environmentally friendly fluid. The total configuration is a combination of a transcritical refrigeration cycle coupled to a Brayton cycle with recompression, which is fed by a biomass boiler. The examined system, at nominal operating conditions, produces refrigeration at 5 °C, and heating at 45 °C and 80 °C. Additionally, the system can be converted into a trigeneration system where the two heating outputs are produced at the same temperature level. The system was studied parametrically by changing the following seven critical parameters: turbine inlet temperature, high pressure, medium pressure, heat exchanger effectiveness, refrigeration temperature, heat rejection temperature and high heating temperature. In nominal operating conditions, the system energy and exergy efficiencies were 78.07% and 26.29%, respectively. For a heat input of 100 kW, the net power production was 24.50 kW, the refrigeration production was 30.73 kW, while the low and high heating production was 9.24 kW and 13.60 kW, respectively. The analysis was conducted with a developed model in Engineering Equation Solver.
Fast Calculation of Transient Thermal Stress in Surge Lines with Thermal Stratification Region
Authors: Gao Bingjun , Li Mingze , Yu Chulin , Li Yan
Published: April 2021

Nuclear power plants applying for license renewal are required to undertake a fatigue assessment of various components, which demands the fast calculation of transient thermal stress of the fatigue-sensitive components such as surge lines. In this study, the strategy of Green’s function database construction for the thermal stratification region was investigated, and the thermal stress calculation was shown to be more accurate by dividing the flow cases and thermal zones. Furthermore, a method to determine the fluid temperature for the thermal zones of the thermal stratification region was studied. In addition to the temperature of the hot leg fluid and the outlet fluid from the pressurizer, seven thermocouples installed on the outside wall in the thermal stratification region were used to determine the fluid temperature of thermal zones with stratification, and the calculation result of the fluid temperature was verified with the temperature Green’s functions.
Seismic Response of Skewed Integral Abutment Bridges under Near-Fault Ground Motions, Including Soil–Structure Interaction
Authors: Zhao Qiuhong , Dong Shuo , Wang Qingwei
Published: April 2021

Studies on the seismic response of skewed integral abutment bridges have mainly focused on response under far-field non-pulse-type ground motions, yet the large amplitude and long-period velocity pulses in near-fault ground motions might have significant impacts on bridge seismic response. In this study, the nonlinear dynamic response of an skewed integral abutment bridge (SIAB) under near-fault pulse and far-fault non-pulse type ground motions are analyzed considering the soil–structure interaction, along with parametric studies on bridge skew angle and compactness of abutment backfill. For the analyses, three sets of near-fault pulse ground motion records are selected based on the bridge site conditions, and three corresponding far-field non-pulse artificial records are fitted by their acceleration response spectra. The results show that the near-fault pulse type ground motions are generally more destructive than the non-pulse motions on the nonlinear dynamic response of SIABs, but the presence of abutment backfill will mitigate the pulse effects to some extent. Coupling of the longitudinal and transverse displacements as well as rotation of the bridge deck would increase with the skew angle, and so do the internal forces of steel H piles. The influence of the skew angle would be most obvious when the abutment backfill is densely compacted.
Human Dental Pulp Stem Cell Osteogenic Differentiation Seeded on Equine Bone Block with Graphene and Melatonin
Authors: Mancinelli Rosa , Di Filippo Ester Sara , Tumedei Margherita , Marrone Mariangela , Fontana Antonella , Ettorre Valeria , Giordani Silvia , Baldrighi Michele , Iezzi Giovanna , Piattelli Adriano , Fulle Stefania
Published: April 2021

Equine bone blocks have osteogenic effects promoting bone regeneration with biocompatibility and osteoconductivity capacity. Human dental pulp stem cells (hDPSCs) can differentiate into osteoblasts enhancing biomineralization with such scaffolds. Melatonin is able to improve bone health and mediate bone formation. Collagenated equine bone blocks were coated with ammonia-functionalized graphene-oxide (G-N) at two different concentrations (2 μg/mL, G-N2; and 10 μg/mL, G-N10). The homogeneity of G-N coating was checked by Raman spectroscopy, whereas thermogravimetric analysis (TGA) allowed us to quantify the amount of G-N deposited on the blocks. The aim of this study was to investigate in vitro the effect of G-N-coated collagenated equine bone blocks on the proliferation and differentiation of hDPSCs with the addition of a melatonin. This evaluation was determined after 7, 14, and 21 days of culture by the expression of specific microRNAs, RUNX2 and SMAD5 gene expression, osteocalcin levels, and histological analysis. The results showed that equine blocks G-N2 and G-N10 and melatonin gave an optimal cell adhesion as shown by histological analysis, and an increase in the hDPSCs osteogenic potential as confirmed by microRNA and gene expression with an increase in osteocalcin levels. This study suggests that equine bone blocks coated with G-N2 and G-N10 and melatonin promote the osteogenic process.
Evaluation of a Wet Acid Scrubber and Dry Filter Abatement Technologies in Pig Barns by Dynamic Olfactometry
Authors: Conti Cecilia , Tullo Emanuela , Bacenetti Jacopo , Guarino Marcella
Published: April 2021

Livestock activities, in particular swine farms, are sources of odorant compounds that cause conflicts with the neighboring population. Beside the effects on the neighborhood, excessive odor emission can cause discomfort to farm workers. In this context the APPROAch project, aims to test the application of two different air cleaning technologies (a wet acid scrubber and a dry filter) to reduce dust, ammonia and odors, in naturally ventilated pig facilities. The aim of the present study is to evaluate, in a pig farm, the odor removal efficiency of the two tested abatement technologies, based on air samples analyzed by dynamic olfactometry. Odor sampling was carried out at a pig facility involved in the project and brought to the lab within 30 h from sampling, as established by the European Standard EN 13725:2004. Odor concentration was evaluated by dynamic olfactometry using an Olfaktomat-n 6 (PRA-Odournet B.V.—Amsterdam, The Netherlands). The results show that the wet acid scrubber prototype presents an average odor removal efficiency of 16%, whereas dry filter has from limited to no effect. This efficiency could be considered as a good result for a prototype even if further analysis, with longer sampling periods are needed.
Total Potential Optimization Using Metaheuristic Algorithms for Solving Nonlinear Plane Strain Systems
Authors: Toklu Yusuf Cengiz , Bekdaş Gebrail , Yücel Melda , Nigdeli Sinan Melih , Kayabekir Aylin Ece , Kim Sanghun , Geem Zong Woo
Published: April 2021

Total Potential Optimization using Metaheuristic Algorithms (TPO/MA) is an alternative tool for the analysis of structures. It is shown that this emerging method is advantageous in solving nonlinear problems like trusses, tensegrity structures, cable networks, and plane stress systems. In the present study, TPO/MA, which does not need any specific implementation for nonlinearity, is demonstrated to be successfully applied to the analysis of plane strain structures. A numerical investigation is performed using nine different metaheuristic algorithms and an adaptive harmony search in linear analysis of a structural mechanics problem having 8 free nodes defined as design variables in the minimization problem of total potential energy. For nonlinear stress-strain relation cases, two structural mechanics problems, one being a thick-walled pipe and the other being a cantilever retaining wall, are analyzed by employing adaptive harmony search, which was found to be the best one in linear analyses. The nonlinear stress-strain relations considered in these analyses are hypothetical ones due to the lack of any such relationship in the literature. The results have shown that TPO/MA can solve nonlinear plane strain problems that can be encountered as engineering problems in structural mechanics.
Review of Contactless Energy Transfer Concept Applied to Inductive Power Transfer Systems in Electric Vehicles
Authors: Razek Adel
Published: April 2021

Nowadays the groundbreaking tools of contactless energy transfer reveals new opportunities to supply portable devices with electrical energy by eliminating cables and connectors. One of the important applications of such technology is the energy providing to electric and hybrid vehicles, (EV) and (HEV). These contribute to the use of cleaner energy to protect our environment. In the present paper, after exposing the contactless energy transfer (CET) available systems, we examine the appropriateness of these systems for EV. After such exploration, it is shown that the most suitable solution is the inductive power transfer (IPT) issue. We analyze such procedure in general and indicate its main usages. Next, we consider the practice of IPT in EV and the different option in the energy managing in EV and HEV concerning battery charging. Following, we review the modes of using the IPT in immobile case and in on-road running. Following, the modeling issues for the IPT system escorting the vehicle structure are then exposed. Lastly, the electromagnetic compatibility (EMC) and human exposure analyses are assessed involving typical appliance.
A Two-Stage Process for Conversion of Brewer’s Spent Grain into Volatile Fatty Acids through Acidogenic Fermentation
Authors: Guarda Eliana C. , Oliveira Ana Catarina , Antunes Sílvia , Freitas Filomena , Castro Paula M. L. , Duque Anouk F. , Reis Maria A. M.
Published: April 2021

This work is focused on the valorization of brewer’s spent grains (BSG) into volatile fatty acids (VFA) through acidogenic fermentation. VFAs are building blocks for several applications, such as bioplastics’ production. Using acid hydrolysis as pre-treatment, several batch assays were performed and the impact of organic load (OL) and pH on VFA production from BSG hydrolysate was assessed. Regardless of the condition, the produced acids were mainly butyric and acetic acids followed by propionic acid. The OL had a direct impact on the total organic acid concentration with higher concentrations at the highest OL (40 gCOD L−1). pH affected the concentration of individual organic acid, with the highest fermentation products (FP) diversity attained at pH 5.0 and OL of 40 gCOD L−1. To assess the potential application of organic acids for biopolymers (such as polyhydroxyalkanoates) production, the content in hydroxybutyrate (HB) and hydroxyvalerate (HV) monomers was estimated from the respective precursors produced at each pH and OL. The content in HV precursors increased with pH, with a maximum at pH 6.0 (ca. 16% C-mol basis). The acidogenic fermentation of BSG hydrolysate was also assessed in continuous operation, using an expanded granular sludge bed reactor (EGSB). It was shown that the BSG hydrolysate was successfully converted to VFAs without pH control, achieving higher productivities than in the batch operation mode.
Thymoquinone, the Most Prominent Constituent of Nigella Sativa, Attenuates Liver Damage in Streptozotocin-Induced Diabetic Rats via Regulation of Oxidative Stress, Inflammation and Cyclooxygenase-2 Protein Expression
Authors: Almatroodi Saleh A. , Alnuqaydan Abdullah M. , Alsahli Mohammed A. , Khan Amjad Ali , Rahmani Arshad Husain
Published: April 2021

Diabetes mellitus (DM) is a multifaceted metabolic disorder that results in dysfunction and failure of various organs. The present study aimed to evaluate the role of Thymoquinone (TQ), on antidiabetic, oxidative stress, and anti-inflammatory activities in streptozotocin (STZ)-induced (55 mg/kg b.w) diabetic rats. TQ was orally given for 8 consecutive weeks at dose of 150 mg/kg b.w. The blood glucose, insulin, total cholesterol, triglycerides, liver function enzymes, high density lipoprotein (HDL)-cholesterol, and low-density lipoprotein (LDL)-cholesterol levels were measured accordingly in control, diabetes control (DC), and TQ-treatment groups. These experiments confirmed that TQ conserves the insulin level (0.4 ng/mL vs. 0.23 ng/mL), fasting blood glucose (146 ± 7 mg/dL vs. 225 ± 5 mg/dL), and HbA1c (7.5% vs. 10.6%) quite considerably as compared to DC animals. Our results also confirmed that TQ treatment conserves the body weight and lipid profile significantly in STZ-treated animals as compared to the DC group. Moreover, the antioxidant enzymes (GSH, SOD, GST, and CAT) levels decreased, liver function enzymes (ALT, AST, and ALP), lipid peroxidation and inflammatory markers (TNF-α, CRP, IL-1β, IL-6) increased by STZ treatment, that is significantly restored after TQ treatment. As compared to untreated animals, TQ restored the hepatocytes architectural changes and collagen fibers and cox-2 protein expression in liver tissues as evaluated by hematoxylin and eosin, Masson’s trichrome, and immunohistochemistry staining. Taken together, all these findings indicated that TQ ameliorates glucose level and lipid metabolism. It restores liver function, antioxidant enzymes, anti-inflammatory markers, and maintains hepatocytes architecture in STZ-induced diabetes mellitus rats. Here, in this study, we have demonstrated for the first time the role of TQ in the reduction of the expression of cyclooxygenase-2 and fibrosis formation in diabetic rats. Based on the findings, the study suggests that TQ is a novel natural drug with a wide range of clinical applications including the management of diabetes mellitus.
Using a Human Interviewer or an Automatic Interviewer in the Evaluation of Patients with AD from Speech
Authors: Alonso-Hernández Jesús B. , Barragán-Pulido María Luisa , Gil-Bordón José Manuel , Ferrer-Ballester Miguel Ángel , Travieso-González Carlos M.
Published: April 2021

Currently, there are more and more frequent studies focused on the evaluation of Alzheimer’s disease (AD) from the automatic analysis of the speech of patients, in order to detect the presence of the disease in an individual or for the evolutionary control of the disease. However, studies focused on analyzing the effect of the methodology used to generate the spontaneous speech of the speaker who undergoes this type of analysis are rare. The objective of this work is to study two different strategies to facilitate the generation of the spontaneous speech of a speaker for further analysis: the use of a human interviewer that promotes the generation of speech through an interview and the use of an automatic system (an automatic interviewer) that invites the speaker to describe certain visual stimuli. In this study, a database called Cross-Sectional Alzheimer Prognosis R2019 has been created, consisting of speech samples from speakers recorded using both methodologies. The speech recordings have been studied through a feature extraction based on five basic temporal measurements. This study demonstrates the discriminatory capacity between the speakers with AD and the control subjects independent of the strategy used in the generation of spontaneous speech. These results are promising and can serve as a basis for knowing the effectiveness and extension of automated interview processes, especially in telemedicine and telecare scenarios.
Nondestructive Contactless Monitoring of Damage in Joints between Composite Structural Components Incorporating Sensing Elements via 3D-Printing
Authors: Dimogianopoulos Dimitrios G. , Mouzakis Dionysios E.
Published: April 2021

A vibration-testing framework for detecting and identifying failing joints between composite structural members without dedicated equipment (accelerometers, amplifiers) or time-consuming system modeling methods is introduced. The sensing element is a 2826MB Metglas® magnetoelastic strip embedded in one of the members during its 3D-printing (layer-by-layer) fabrication process in fused deposition modeling mode. External dynamic loading of the structure causes changes to the strip’s magnetization, thus inducing voltage to a nearby placed coil in a contactless manner. The resulting signal depends on the structure’s behavior under loading (and therefore its condition), and may be recorded without amplification or filtering by conventional oscilloscopes. Its frequency analysis reveals patterns of shifted frequency and/or altered damping at specific modes attributed to failing joints. Apart from yielding results using less dedicated equipment than other vibration-testing methods, the current framework offers two additional benefits: (i) Excitation may be applied to the same structural point for all monitored joints; (ii) estimation of damping values for a given mode does not have to rely on empirical or system modelling techniques (both requiring dedicated expertise). Test runs with structures formed by two or three composite slabs joined in-series indicate promising results with successful detection and identification of failing joints.
From Brown-Field to Future Industrial Networks, a Case Study
Authors: Lavassani Mehrzad , Åkerberg Johan , Björkman Mats
Published: April 2021

The network infrastructures in the future industrial networks need to accommodate, manage and guarantee performance to meet the converged Internet technology (IT) and operational technology (OT) traffics requirements. The pace of IT–OT networks development has been slow despite their considered benefits in optimizing the performance and enhancing information flows. The hindering factors vary from general challenges in performance management of the diverse traffic for green-field configuration to lack of outlines for evolving from brown-fields to the converged network. Focusing on the brown-field, this study provides additional insight into a brown-field characteristic to set a baseline that enables the subsequent step development towards the future’s expected converged networks. The case study highlights differences between real-world network behavior and the common assumptions for analyzing the network traffic covered in the literature. Considering the unsatisfactory performance of the existing methods for characterization of brown-field traffic, a performance and dynamics mixture measurement is proposed. The proposed method takes both IT and OT traffic into consideration and reduces the complexity, and consequently improves the flexibility, of performance and configuration management of the brown-field.
Special Issue “Fundamentals and Recent Advances in Epitaxial Graphene on SiC”
Authors: Shtepliuk Ivan , Yakimova Rositsa
Published: April 2021

The aim of this Special Issue is to provide a scientific platform for recognized experts in the field of epitaxial graphene on SiC to present their recent studies towards a deeper comprehension of growth mechanisms, property engineering and device processing. This Special Issue gives readers the possibility to gain new insights into the nature of buffer layer formation, control of electronic properties of graphene and usage of epitaxial graphene as a substrate for deposition of different substances, including metals and insulators. We believe that the papers published within the current Special Issue develop cumulative knowledge on matters related to device-quality epaxial graphene on SiC, bringing this material closer to realistic practical applications.
Gamification in IT Service Management: A Systematic Mapping Study
Authors: Trinidad Manuel , Orta Elena , Ruiz Mercedes
Published: April 2021

Despite the benefits of adopting IT Service Management (ITSM) reference models, such initiatives do not always produce the expected results. The research literature in this area concludes that motivation, engagement, skills, experience, performance and willingness to change of the personnel involved are among the critical factors for an effective ITSM implementation. Gamification has the capability to improve people’s motivation and engagement and to drive people’s behavior to meet the objectives set. Besides, gamification is widely used in learning systems for increasing students’ skills and competences. In the last years, many researchers have added gamification to their process improvement initiatives to increase the motivation and engagement of process participants and to address their behavior throughout the process. Thus, we consider that adopting gamification in ITSM processes can be an interesting area of study. In this paper, we conducted a systematic mapping study to analyze the actual state of research in the field of ITSM gamification and identify the key challenges that justify future research. The results of our study highlight the positive impact of adopting gamification in ITSM processes and that ITSM gamification is a novel an attractive research area with many action possibilities.
System of Systems Lifecycle Management—A New Concept Based on Process Engineering Methodologies
Authors: Kozma Dániel , Varga Pál , Larrinaga Felix
Published: April 2021

In order to tackle interoperability issues of large-scale automation systems, SOA (Service-Oriented Architecture) principles, where information exchange is manifested by systems providing and consuming services, have already been introduced. However, the deployment, operation, and maintenance of an extensive SoS (System of Systems) mean enormous challenges for system integrators as well as network and service operators. The existing lifecycle management approaches do not cover all aspects of SoS management; therefore, an integrated solution is required. The purpose of this paper is to introduce a new lifecycle approach, namely the SoSLM (System of Systems Lifecycle Management). This paper first provides an in-depth description and comparison of the most relevant process engineering methodologies and ITSM (Information Technology Service Management) frameworks, and how they affect various lifecycle management strategies. The paper’s novelty strives to introduce an Industry 4.0-compatible PLM (Product Lifecycle Management) model and to extend it to cover SoS management-related issues on well-known process engineering methodologies. The presented methodologies are adapted to the PLM model, thus creating the recommended SoSLM model. This is supported by demonstrations of how the IIoT (Industrial Internet of Things) applications and services can be developed and handled. Accordingly, complete implementation and integration are presented based on the proposed SoSLM model, using the Arrowhead framework that is available for IIoT SoS.
Thermodynamic Performance of Adsorption Working Pairs for Low-Temperature Waste Heat Upgrading in Industrial Applications
Authors: Frazzica Andrea , Palomba Valeria , Dawoud Belal
Published: April 2021

The present work aims at the thermodynamic analysis of different working pairs in adsorption heat transformers (AdHT) for low-temperature waste heat upgrade in industrial processes. Two different AdHT configurations have been simulated, namely with and without heat recovery between the adsorbent beds. Ten working pairs, employing different adsorbent materials and four different refrigerants, have been compared at varying working boundary conditions. The effects of heat recovery and the presence of a temperature gradient for heat transfer between sinks/sources and the AdHT components have been analyzed. The achieved results demonstrate the possibility of increasing the overall performance when internal heat recovery is implemented. They also highlight the relevant role played by the existing temperature gradient between heat transfer fluids and components, that strongly affect the real operating cycle of the AdHT and thus its expected performance. Both extremely low, i.e., 40–50 °C, and low (i.e., 80 °C) waste heat source temperatures were investigated at variable ambient temperatures, evaluating the achievable COP and specific energy. The main results demonstrate that optimal performance can be achieved when 40–50 K of temperature difference between waste heat source and ambient temperature are guaranteed. Furthermore, composite sorbents demonstrated to be the most promising adsorbent materials for this application, given their high sorption capacity compared to pure adsorbents, which is reflected in much higher achievable specific energy.
Atlantic Hurricane Activity Prediction: A Machine Learning Approach
Authors: Asthana Tanmay , Krim Hamid , Sun Xia , Roheda Siddharth , Xie Lian
Published: April 2021

Long-term hurricane predictions have been of acute interest in order to protect the community from the loss of lives, and environmental damage. Such predictions help by providing an early warning guidance for any proper precaution and planning. In this paper, we present a machine learning model capable of making good preseason-prediction of Atlantic hurricane activity. The development of this model entails a judicious and non-linear fusion of various data modalities such as sea-level pressure (SLP), sea surface temperature (SST), and wind. A Convolutional Neural Network (CNN) was utilized as a feature extractor for each data modality. This is followed by a feature level fusion to achieve a proper inference. This highly non-linear model was further shown to have the potential to make skillful predictions up to 18 months in advance.
The Hsp60 Protein of Helicobacter Pylori Exhibits Chaperone and ATPase Activities at Elevated Temperatures
Authors: Mendoza Jose A. , Ignacio Julian L. , Buckley Christopher M.
Published: April 2021

The heat-shock protein, Hsp60, is one of the most abundant proteins in Helicobacter pylori. Given its sequence homology to the Escherichia coli Hsp60 or GroEL, Hsp60 from H. pylori would be expected to function as a molecular chaperone in this organism. H. pylori is a type of bacteria that grows on the gastric epithelium, where the pH can fluctuate between neutral and 4.5, and the intracellular pH can be as low as 5.0. We previously showed that Hsp60 functions as a chaperone under acidic conditions. However, no reports have been made on the ability of Hsp60 to function as a molecular chaperone under other stressful conditions, such as heat stress or elevated temperatures. We report here that Hsp60 could suppress the heat-induced aggregation of the enzymes rhodanese, malate dehydrogenase, citrate synthase, and lactate dehydrogenase. Moreover, Hsp60 was found to have a potassium and magnesium-dependent ATPase activity that was stimulated at elevated temperatures. Although, Hsp60 was found to bind GTP, the hydrolysis of this nucleotide could not be observed. Our results show that Hsp60 from H. pylori can function as a molecular chaperone under conditions of heat stress.
Inborn Errors of Immunity and Cancer
Authors: Tiri Alessandra , Masetti Riccardo , Conti Francesca , Tignanelli Anna , Turrini Elena , Bertolini Patrizia , Esposito Susanna , Pession Andrea
Published: April 2021

Inborn Errors of Immunity (IEI) are a heterogeneous group of disorders characterized by a defect in the function of at least one, and often more, components of the immune system. The aim of this narrative review is to discuss the epidemiology, the pathogenesis and the correct management of tumours in patients with IEI. PubMed was used to search for all of the studies published over the last 20 years using the keywords: “inborn errors of immunity” or “primary immunodeficiency” and “cancer” or “tumour” or “malignancy”. Literature analysis showed that the overall risk for cancer in children with IEI ranges from 4 to 25%. Several factors, namely, age of the patient, viral infection status and IEI type can influence the development of different cancer types. The knowledge of a specific tumour risk in the presence of IEI highlights the importance of a synergistic effort by immunologists and oncologists in tracking down the potential development of cancer in known IEI patients, as well as an underlying IEI in patients with newly diagnosed cancers. In the current genomic era, the creation of an international registry of IEI cases integrated with malignancies occurrence information is fundamental to optimizing the diagnostic process and to evaluating the outcomes of new therapeutic options, with the hope to obtain a better prognosis for these patients.
The Role of RNA in DNA Breaks, Repair and Chromosomal Rearrangements
Authors: Murashko Matvey Mikhailovich , Stasevich Ekaterina Mikhailovna , Schwartz Anton Markovich , Kuprash Dmitriy Vladimirovich , Uvarova Aksinya Nicolaevna , Demin Denis Eriksonovich
Published: April 2021

Incorrect reparation of DNA double-strand breaks (DSB) leading to chromosomal rearrangements is one of oncogenesis’s primary causes. Recently published data elucidate the key role of various types of RNA in DSB formation, recognition and repair. With growing interest in RNA biology, increasing RNAs are classified as crucial at the different stages of the main pathways of DSB repair in eukaryotic cells: nonhomologous end joining (NHEJ) and homology-directed repair (HDR). Gene mutations or variation in expression levels of such RNAs can lead to local DNA repair defects, increasing the chromosome aberration frequency. Moreover, it was demonstrated that some RNAs could stimulate long-range chromosomal rearrangements. In this review, we discuss recent evidence demonstrating the role of various RNAs in DSB formation and repair. We also consider how RNA may mediate certain chromosomal rearrangements in a sequence-specific manner.