bluuebunny/crossref_metadata_2025 huggingface dataset:
Inertial Sensing of the Abdominal Wall Kinematics during Diaphragmatic Breathing in Head Standing
Authors: Rousanoglou Elissavet , Foskolou Apostolina , Emmanouil Analina , Boudolos Konstantinos
Published: February 2024

Head standing (HS) in concurrence with diaphragmatic breathing is an atypical deviation from daily activity, yet commonly practiced. The study aimed at the inertially sensed effect of diaphragmatic versus normal breathing on the abdomen wall kinematics during HS. Twenty-eight men and women maintained HS and erect standing (ES) under normal and diaphragmatic breathing. An inertial sensor (LORD MicroStrain®, 3DM-GX3®-45, 2 cm above the umbilicus, 100 Hz, MicroStrain, Williston, VT, USA) recorded the 3D abdomen wall angular displacement (AD) (bandpass filter (0.1–0.5 Hz)). ANOVAs (p ≤ 0.05, SPSS 28.0) were applied to the extracted variables (AD path: magnitude, individual variability-%CVind, and diaphragmatic to normal ratio). Reliability measures (ICC and %SEM) and the minimal detectable change (%MDC90) were estimated. Diaphragmatic breathing increased the AD path (p ≤ 0.05) with the diaphragmatic to normal ratio being lower in HS (p ≤ 0.05). The similar AD time series (cross-correlations at p ≤ 0.05) and the ICCs (>0.80) indicated excellent reliability with the similar across conditions %CVind (p ≤ 0.05), further enhancing reliability. The %MDC90 was consistently higher than the %SEM upper boundary, indicating the differences as “real” ones. The results contribute to the limited data concerning a widely practiced atypical deviation from daily activity, as HS in concurrence with diaphragmatic breathing.
In Association with Other Risk Factors, Smoking Is the Main Predictor for Lower Transcutaneous Oxygen Pressure in Type 2 Diabetes
Authors: Bulum Tomislav , Brkljačić Neva , Tičinović Ivančić Angelika , Čavlović Maja , Prkačin Ingrid , Tomić Martina
Published: February 2024

Type 2 diabetes mellitus (T2DM) significantly increases the risk of peripheral artery disease (PAD), and diabetes is the leading cause of nontraumatic amputations. This study investigated the risk factors for transcutaneous oxygen pressure (TcPO2) in T2DM, a noninvasive method to quantify skin oxygenation and the underlying microvascular circulation. The study included 119 T2DM patients (91 male/28 female). TcPO2 measurements were conducted with the Tina TCM4 Series transcutaneous monitor (Radiometer, Copenhagen, Sweden) and skin electrodes. Patients with TcPO2 < 40 mmHg were younger (p = 0.001), had significantly higher systolic blood pressure (SBP) (p = 0.023), glycated hemoglobin (HbA1c) (p = 0.013), fasting plasma glucose (fPG) (p = 0.038), total cholesterol (p = 0.006), LDL cholesterol (p = 0.004), and had more frequent smoking habits (p = 0.001) than those with TcPO2 ≥ 40 mmHg. The main predictors for the TcPO2 value (R2 = 0.211) obtained via stepwise regression analysis were age, smoking, SBP, HbA1c, fPG, and total and LDL cholesterol. Among all the listed predictors, smoking, HbA1c, and LDL cholesterol were found to be the most significant, with negative parameter estimates of −3.051310 (p = 0.0007), −2.032018 (p = 0.0003), and −2.560353 (p = 0.0046). The results of our study suggest that in association with other risk factors, smoking is the main predictor for lower TcPO2 in T2DM.
Mapping Changes in Glutamate with Glutamate-Weighted MRI in Forced Swim Test Model of Depression in Rats
Authors: Lee Donghoon , Woo Chul-Woong , Heo Hwon , Ko Yousun , Jang Ji Sung , Na Seongwon , Kim Nari , Woo Dong-Cheol , Kim Kyung Won , Lee Do-Wan
Published: February 2024

Chemical exchange saturation transfer with glutamate (GluCEST) imaging is a novel technique for the non-invasive detection and quantification of cerebral Glu levels in neuromolecular processes. Here we used GluCEST imaging and 1H magnetic resonance spectroscopy (1H MRS) to assess in vivo changes in Glu signals within the hippocampus in a rat model of depression induced by a forced swim test. The forced swimming test (FST) group exhibited markedly reduced GluCEST-weighted levels and Glu concentrations when examined using 1H MRS in the hippocampal region compared to the control group (GluCEST-weighted levels: 3.67 ± 0.81% vs. 5.02 ± 0.44%, p < 0.001; and Glu concentrations: 6.560 ± 0.292 μmol/g vs. 7.133 ± 0.397 μmol/g, p = 0.001). Our results indicate that GluCEST imaging is a distinctive approach to detecting and monitoring Glu levels in a rat model of depression. Furthermore, the application of GluCEST imaging may provide a deeper insight into the neurochemical involvement of glutamate in various psychiatric disorders.
Unravelling Insights into the Evolution and Management of SARS-CoV-2
Authors: Mushebenge Aganze Gloire-Aimé , Ugbaja Samuel Chima , Mbatha Nonkululeko Avril , Khan Rene B. , Kumalo Hezekiel M.
Published: February 2024

Worldwide, the COVID-19 pandemic, caused by the brand-new coronavirus SARS-CoV-2, has claimed a sizable number of lives. The virus’ rapid spread and impact on every facet of human existence necessitate a continuous and dynamic examination of its biology and management. Despite this urgency, COVID-19 does not currently have any particular antiviral treatments. As a result, scientists are concentrating on repurposing existing antiviral medications or creating brand-new ones. This comprehensive review seeks to provide an in-depth exploration of our current understanding of SARS-CoV-2, starting with an analysis of its prevalence, pathology, and evolutionary trends. In doing so, the review aims to clarify the complex network of factors that have contributed to the varying case fatality rates observed in different geographic areas. In this work, we explore the complex world of SARS-CoV-2 mutations and their implications for vaccine efficacy and therapeutic interventions. The dynamic viral landscape of the pandemic poses a significant challenge, leading scientists to investigate the genetic foundations of the virus and the mechanisms underlying these genetic alterations. Numerous hypotheses have been proposed as the pandemic has developed, covering various subjects like the selection pressures driving mutation, the possibility of vaccine escape, and the consequences for clinical therapy. Furthermore, this review will shed light on current clinical trials investigating novel medicines and vaccine development, including the promising field of drug repurposing, providing a window into the changing field of treatment approaches. This study provides a comprehensive understanding of the virus by compiling the huge and evolving body of knowledge on SARS-CoV-2, highlighting its complexities and implications for public health, and igniting additional investigation into the control of this unprecedented global health disaster.
Non-Contact Blood Pressure Estimation Using Forehead and Palm Infrared Video
Authors: Stogiannopoulos Thomas , Mitianoudis Nikolaos
Published: February 2024

This study investigates the potential of low-cost infrared cameras for non-contact monitoring of blood pressure (BP) in individuals with fragile health, particularly the elderly. Previous research has shown success in developing non-contact BP monitoring using RGB cameras. In this study, the Eulerian Video Magnification (EVM) technique is employed to enhance minor variations in skin pixel intensity in specific facial regions captured by an infrared camera from the forehead and palm. The primary focus of this study is to explore the possibility of using infrared cameras for non-contact BP monitoring under low-light or night-time conditions. We have successfully shown that by employing a series of straightforward signal processing techniques and regression analysis, we were able to achieve commendable outcomes in our experimental setup. Specifically, we were able to surpass the stringent accuracy standards set forth by the British Hypertension Society (BHS) and the Association for the Advancement of Medical Instrumentation (AAMI) protocol.
Design and Optimization of an Adaptive Knee Joint Orthosis for Biomimetic Motion Rehabilitation Assistance
Authors: Liu Kun , Ji Shuo , Liu Yong , Zhang Shizhong , Dai Lei
Published: February 2024

In this paper, an adaptive knee joint orthosis with a variable rotation center for biomimetic motion rehabilitation assistance suitable for patients with knee joint movement dysfunction is designed. Based on the kinematic information of knee joint motion obtained by a motion capture system, a Revolute-Prismatic-Revolute (RPR) model is established to simulate the biomimetic motion of the knee joint, then a corresponding implementation for repetitively driving the flexion–extension motion of the knee joint, mainly assembled by a double-cam meshing mechanism, is designed. The pitch curve of each cam is calculated based on the screw theory. During the design process, size optimization is used to reduce the weight of the equipment, resulting in a reduction from 1.96 kg to 1.16 kg, achieving the goal of lightweight equipment. Finally, a prototype of the designed orthosis with the desired biomimetic rotation function is prepared and verified. The result shows that the rotation center of the prototype can achieve biomimetic motion coincident with the rotation center of an active knee joint, which can successfully provide rehabilitation assistance for the knee joint flexion–extension motion.
Current Knowledge of Helminths of Wild Birds in Ecuador
Authors: Carrera-Játiva Patricio D. , Jiménez-Uzcátegui Gustavo
Published: February 2024

Parasitic helminths are diverse in wild birds globally, but knowledge about helminths in Ecuadorian avifauna is still fragmentary. In the present review, records about helminths (Platyhelminthes, Nematoda, and Acanthocephala) in Ecuadorian wild birds is presented. A systematic search was carried out using the PRISMA (Preferred Reporting Items for Systematic Review and Meta-Analysis) guideline to compile and summarize the available literature on helminths in wild birds in Ecuador. Data were obtained from ten scientific articles, eight undergraduate theses, and one doctoral dissertation, published between 1966 to 2022. Forty helminth taxa were recorded and information about the host species, site of infection, and location are provided. Nematodes of the genus Ascaridia Dujardin, 1844 were the helminth taxa with the greatest number of records in birds, parasitizing 16 avian species. Also, the Rock Dove (Columba livia; Gmelin, 1789) was the avian species with the greater number of helminth records (n = 11). This review serves as a compendium for future ecological and epidemiological studies on helminths in wild birds in Ecuador and South America.
Correlation between Morphological and Hemodynamic Parameters of Carotid Arteries and Cerebral Vasomotor Reactivity
Authors: Stoisavljevic Stefan , Stojanovic Milica , Zdraljevic Mirjana , Aleksic Vuk , Pekmezovic Tatjana , Mijajlovic Milija
Published: February 2024

The function of cerebral small vessels can be assessed using cerebral vasomotor reactivity (VMR). Our aim in this retrospective cross-sectional study was to investigate a correlation between carotid artery stenosis measured through ultrasonographic morphological and hemodynamic parameters and cerebral VMR. A total of 285 patients (125 males; mean age 54) were included. The breath-holding index (BHI) was used to evaluate cerebral VMR. Ultrasonographic carotid artery parameters were collected: the presence and characteristics of carotid plaques, the degree of carotid diameter stenosis, intima–media thickness (IMT), peak systolic velocity (PSV), and end diastolic velocity (EDV). Additionally, hemodynamic parameters of the middle cerebral artery (MCA) were evaluated, including the mean flow velocity (MFV) and pulsatility index (PI). The following was collected from patients’ medical histories: age, gender, and vascular risk factors. A negative correlation between the BHI and age (r = −0.242, p < 0.01), BHI and the presence of carotid plaques, BHI and IMT (r = −0.203, p < 0.01), and BHI and the PI of MCA on both sides (r = −0.268, p < 0.01) was found. We found a positive correlation between the BHI in the left MCA and EDV in the left internal carotid artery (r = 0.121, p < 0.05). This study shows the correlation between cerebral VMR and carotid stenosis but indicates a higher influence of morphological parameters on VMR values.
Probing the Bottleneck of Awareness Formed by Foveal Crowding: A Neurophysiological Study
Authors: Siman-Tov Ziv , Lev Maria , Polat Uri
Published: February 2024

Crowding occurs when an easily identified isolated stimulus is surrounded by stimuli with similar properties, making it very difficult to identify. Crowding is suggested as a mechanism that creates a bottleneck in object recognition and awareness. Recently, we showed that brief presentation times at the fovea resulted in a significant crowding effect on target identification, impaired the target’s color awareness, and resulted in a slower reaction time. However, when tagging the target with a red letter, the crowding effect is abolished. Crowding is widely considered a grouping; hence, it is pre-attentive. An event-related potential (ERP) study that investigated the spatial–temporal properties of crowding suggested the involvement of higher-level visual processing. Here, we investigated whether ERP’s components may be affected by crowding and tagging, and whether the temporal advantage of ERP can be utilized to gain further information about the crowding mechanism. The participants reported target identification using our standard foveal crowing paradigm. It is assumed that crowding occurs due to a suppressive effect; thus, it can be probed by changes in perceptual (N1, ~160 ms) and attentive (P3 ~300–400 ms) components. We found a suppression effect (less negative ERP magnitude) in N1 under foveal crowding, which was recovered under tagging conditions. ERP’s amplitude components (N1 and P3) and the behavioral proportion correct are highly correlated. These findings suggest that crowding is an early grouping mechanism that may be combined with later processing involving the segmentation mechanism.
Harnessing Path Optimization to Enhance the Strength of Three-Dimensional (3D) Printed Concrete
Authors: Jiang Xiongzhi , Li Yujia , Yang Zhe , Li Yangbo , Xiong Bobo
Published: February 2024

The path-dependent strength of three-dimensional printed concrete (3DPC) hinders further engineering application. Printing path optimization is a feasible solution to improve the strength of 3DPC. Here, the mix ratio of 3DPC was studied to print standard concrete specimens with different printing paths using our customized concrete 3D printer, which features fully sealed extrusion and ultrathin nozzles. These paths include crosswise, vertical, arched, and diagonal patterns. Their flexural and compressive strengths were tested. In order to verify the tested results and expose the mechanism of strength enhancement, digital image correlation (DIC) was used to capture the dynamic gradual fracture in the flexural tests. Also, the meso- and microstructures of the 3D-printed concrete specimens were pictured. The results reported here show that arched-path concrete has 30% more flexural strength than others because it makes better use of filament-wise strength. The findings here provide a pathway to improve the strength of 3D-printed concrete by path optimization, boosting 3DPC’s extensive application in civil engineering.
Improvement of the Cracking Moment-Based Asphalt Mixture Splitting Test Method and Splitting Strength Research
Authors: Guan Hongxin , Pan Wang , Yang Hairong , Yang Yuxuan
Published: February 2024

The asphalt mixture splitting test is one of the most important methods for measuring asphalt’s tensile properties. To characterize the limitations of the traditional splitting test and the influence of the specimen size and loading conditions on the accuracy of the test, the factors affecting the strength of the splitting test were analyzed to reveal the splitting failure state and establish a unified representation model between the splitting and direct tensile tests. Initially, the moment of specimen cracking was taken as a key indicator, combined with image processing technology, to establish a set of criteria to judge the splitting test. Subsequently, standardized splitting tests were conducted and compared to tests of different specimen sizes and loading methods. Based on the octahedral strength theory, the stress points before and after the improved test were compared to the existing failure criteria. Direct tensile and splitting tests were conducted at different rates, and a unified strength–rate function model was established, realizing the unified representation of direct tensile and splitting tests. The research results indicate that the standardized splitting test is prone to the phenomenon wherein the specimen end face cracks before the center, affecting the accuracy of the test and potentially leading to redundant material strength evaluations. Using a loading method with a “35 mm specimen thickness” and a “0.3 mm rubber gasket + 12.7 mm arc-shaped batten” can essentially achieve the test hypothesis of “cracking at the center first”, resulting in less discrete outcomes and closer alignment to the three-dimensional stress failure state. The tensile and splitting strengths are both power function relationships with the rate as the independent variable, establishing a unified function model of the tensile and failure strengths. The research provides a more reliable testing method and calculation model for asphalt pavement structure design, and it also provides an effective basis for the improvement of splitting tests on materials such as concrete and rock.
Damage Detection of Gantry Crane with a Moving Mass Using Artificial Neural Network
Authors: Safaei Mohammad , Hejazian Mahsa , Pedrammehr Siamak , Pakzad Sajjad , Ettefagh Mir Mohammad , Fotouhi Mohammad
Published: February 2024

Gantry cranes play a pivotal role in various industrial applications, and their reliable operation is paramount. While routine inspections are standard practice, certain defects, particularly in less accessible components, remain challenging to detect early. In this study, first a finite element model is presented, and the damage is introduced using random changes in the stiffness of different parts of the structure. Contrary to the assumption of inherent reliability, undetected defects in crucial structural elements can lead to catastrophic failures. Then, the vibration equations of healthy and damaged models are analyzed to find the displacement, velocity, and acceleration of the different crane parts. The learning vector quantization neural network is used to train and detect the defects. The output is the location of the damage and the damage severity. Noisy data are then used to evaluate the network performance robustness. This research also addresses the limitations of traditional inspection methods, providing early detection and classification of defects in gantry cranes. The study’s relevance lies in the need for a comprehensive and efficient damage detection method, especially for components not easily accessible during routine inspections.
Wind-Induced Vibration Analysis of a Pentagonal Three–Four Strut Hybrid Open-Type Cable Dome
Authors: Lv Hui , Liu Dewang , Shao Lian , Liu Yaopeng , Zhu Zhongyi , Dong Shilin , Zhong Yanfen
Published: February 2024

Previous research has confirmed that the newly proposed pentagonal three–four strut hybrid cable dome exhibits superior static performance compared to traditional cable domes, though its dynamic characteristics still require further study. Cable domes are wind-sensitive structures, and the results of a wind-induced vibration analysis are beneficial for the selection and construction of cable domes. In this study, a finite element model of a new open-type cable dome with a span of 120 m is established. The MATLAB 2017a programming language is employed to simulate pulsating winds, followed by a nonlinear dynamic analysis to analyze the wind-induced vibrations of the structure. The reliability of the pulsating wind model is confirmed by comparing the simulated spectrum with the target spectrum. Moreover, a wind-induced vibration time history analysis is performed to obtain the node displacement and internal force of components wind vibration coefficients, aiding in the approximation of pulsating winds with average winds in a wind-resistant design. Furthermore, a parametric analysis is carried out, ranking nodes and components based on sensitivity. The result shows that the structure exhibits the strongest wind resistance when the rise–span ratio is f/l=0.07 and the thickness–span ratio is h/l=0.08. Notably, the outer upper chord node, 2a, and the inner lower chord hoop cable, H1, are identified as the most sensitive node and component within the structure, respectively. Overall, the structure demonstrates excellent wind resistance performance, and the maximum wind vibration coefficient value remains below 3.
Sound Absorption Characteristics of Unsaturated Porous Material Calculated by Modified Transfer Matrix Method
Authors: Yin Yiguo , Guan Wei
Published: February 2024

Porous materials have emerged as one of the most widely employed sound-absorbing materials in practical applications, owing to their excellent sound absorption properties and lightweight nature. Unsaturated porous materials have the potential to achieve superior sound absorption effects and significantly reduce mass. However, current research on the sound absorption performance of unsaturated porous materials is limited. This paper used a modified transfer matrix method (MTMM), which relies on matrix dimensionality reduction, to analyze the sound absorption characteristics of unsaturated porous materials under various boundary conditions. The study also examines the impact of different parameters, such as material thickness and saturation, on sound absorption performance and compares the results. The findings indicate that when a solid elastic plate is attached to an unsaturated porous elastic plate, the absorption coefficient decreases while the transmission loss increases. Additionally, reducing the thickness of the plate and increasing its porosity and saturation levels lead to an increase in the absorption coefficient and a decrease in the transmission loss.
Modulation of Estrogen Receptor Alpha (ERα) and Tumor Suppressor Gene BRCA1 in Breast Cancer Cells by Bazedoxifene Acetate (BZA)
Authors: Szmyd Monica , Zanib Aisha , Behlow Victoria , Hallman Erin , Pfiffner Samantha , Yaldo Raquel , Prudhomme Nina , Farrar Katelyn , Dinda Sumi
Published: February 2024

Selective estrogen receptor modulators (SERMs) are steroid analogs with dual functionality, acting as partial estrogen receptor agonists to preserve postmenopausal bone density and as estrogen receptor antagonists in breast tissue. Bazedoxifene acetate (BZA) is an FDA-approved, third-generation SERM used in the treatment of osteoporosis in women. It demonstrates potential as a therapeutic option for breast cancer patients undergoing endocrine therapy. Our study aimed to assess BZA’s effects on Estrogen Receptor Alpha (ERα) and tumor suppressor gene BRCA1 in T-47D and MCF-7 breast cancer cells, using Western blots, cellular viability, apoptosis assays, and RT-qPCR. Cells were cultured in 5% charcoal-stripped fetal bovine serum for six days to deplete endogenous steroids. Following a 24 h exposure to 2 µM BZA (optimal concentration determined from 1 nM–2 µM studies), Western blot analyses revealed reduced ERα and BRCA1 protein levels in both cell lines. ERα decreased by 48–63% and BRCA1 by 61–64%, indicating sensitivity to antiestrogens. Cytolocalization of ERα and BRCA1 remained unchanged after BZA and 17-β-estradiol (E2) treatment. ESR1 mRNA expression correlated with Western blot findings. Image cytometric analysis using the stain, propidium iodide, detected decreased cellular proliferation in T-47D and MCF-7 cells following a 6-day treatment ranging from 1 nM to 2 µM BZA. BZA treatment alone led to a tenfold reduction in cellular proliferation compared to estrogen-treated cells, suggesting antiproliferative effects. Understanding BZA’s modulation of BRCA1 and ERα, along with their mechanistic interactions, is vital for comprehending its impact on breast cancer tumor suppressors and hormone receptors.
Editorial: Recent Advances in Deep Learning and Medical Imaging for Cancer Treatment
Authors: Ijaz Muhammad , Woźniak Marcin
Published: February 2024

In the evolving landscape of medical imaging, the escalating need for deep-learningmethods takes center stage, offering the capability to autonomously acquire abstract datarepresentations crucial for early detection and classification for cancer treatment. Thecomplexities in handling diverse inputs, high-dimensional features, and subtle patternswithin imaging data are acknowledged as significant challenges in this technologicalpursuit. This Special Issue, “Recent Advances in Deep Learning and Medical Imagingfor Cancer Treatment”, has attracted 19 high-quality articles that cover state-of-the-artapplications and technical developments of deep learning, medical imaging, automaticdetection, and classification, explainable artificial intelligence-enabled diagnosis for cancertreatment. In the ever-evolving landscape of cancer treatment, five pivotal themes haveemerged as beacons of transformative change. This editorial delves into the realms ofinnovation that are shaping the future of cancer treatment, focusing on five interconnectedthemes: use of artificial intelligence in medical imaging, applications of AI in cancerdiagnosis and treatment, addressing challenges in medical image analysis, advancementsin cancer detection techniques, and innovations in skin cancer classification.
Real-Life Use of [68Ga]Ga-DOTANOC PET/CT in Confirmed and Suspected NETs from a Prospective 5-Year Electronic Archive at an ENETS Center of Excellence: More Than 2000 Scans in More Than 1500 Patients
Authors: Bonazzi Norma , Fortunati Emilia , Zanoni Lucia , Argalia Giulia , Calabrò Diletta , Tabacchi Elena , Allegri Vincenzo , Campana Davide , Andrini Elisa , Lamberti Giuseppe , Di Franco Martina , Casadei Riccardo , Ricci Claudio , Mosconi Cristina , Fanti Stefano , Ambrosini Valentina
Published: February 2024

The recent introduction of novel treatments for advanced neuroendocrine tumors (NETs) and the well-established impact of clinical case discussion within dedicated multidisciplinary teams indicates the need to promote the centralization of rare diseases, such as NENs (neuroendocrine neoplasms). Data on the real-life use of and indications for [68Ga]Ga-DOTANOC PET/CT were collected from a prospective monocentric 5-year electronic archive including consecutive patients with confirmed and suspected NETs (September 2017 to May 2022). Overall, 2082 [68Ga]Ga-DOTANOC PET/CT scans (1685 confirmed NETs, 397 suspected NETs) were performed in 1537 patients. A high positivity rate was observed across different clinical settings (approximately 70%). Approximately 910/2082 scans were requested by the local oncology ward (851 confirmed NETs, 59 suspected NETs). The following observations were found: (i) the detection rate across all indications was 73.2% (higher for staging, peptide receptor radioligand therapy (PRRT) selection, and treatment response assessment); (ii) in suspected NETs, PET was more often positive when based on radiological findings. This systematic data collection in a high-volume diagnostic center represents a reliable cohort reflecting the global trends in the use of [68Ga]Ga-DOTANOC PET/CT for different clinical indications and primary tumor sites, but prompts the need for further multicenter data sharing in such a rare and slowly progressive disease setting.
Pulmonary Metastasectomy for Adrenocortical Carcinoma—Not If, but When
Authors: Carr Shamus R. , Villa Hernandez Frank , Varghese Diana Grace , Choo-Wosoba Hyoyoung , Steinberg Seth M. , Teke Martha E. , Del Rivero Jaydira , Schrump David S. , Hoang Chuong D.
Published: February 2024

Background: Adrenocortical carcinoma (ACC) commonly metastasizes to the lungs, and pulmonary metastasectomy (PM) is utilized due to limited systemic options. Methods: All ACC patients with initially only lung metastases (LM) from a single institution constituted this observational case series. Kaplan-Meier and Cox proportional hazard analyses evaluated the association with potential prognostic factors and outcomes. Overall survival (OS) was calculated from the date of the PM or, in those patients who did not undergo surgery, from the development of LM. Results: A total of 75 ACC patients over a 45-year period met the criteria; 52 underwent PM, and 23 did not. The patients undergoing PM had a median OS of 3.1 years (95% CI: 2.4, 4.7 years) with the 5- and 10-year OS being 35.5% and 32.8%, respectively. The total resected LM did not impact the OS nor the DFS. The patients who developed LM after 11 months from the initial ACC resection had an improved OS (4.2 years; 95% CI: 3.2, NR; p = 0.0096) compared to those developing metastases earlier (2.4 years; 95% CI: 1.6, 2.8). Patients who underwent PM within 11 months of adrenalectomy demonstrated a reduced OS (2.2 years; 95% CI: 1.0, 2.7) compared to those after 11 months (3.6 years, 95% CI: 2.6, NR; p = 0.0045). PM may provide benefit to those patients with LM at presentation (HR: 0.5; p = 0.2827), with the time to first PM as a time-varying covariate. Conclusions: PM appears to have a role in ACC patients. The number of nodules should not be an exclusion factor. Patients developing LM within a year of primary tumor resection may benefit from waiting before further surgeries, which may provide additional insight into who may benefit from PM.
Polygenic Risk Score in Predicting Esophageal, Oropharyngeal, and Hypopharynx Cancer Risk among Taiwanese Population
Authors: Huang Yu-Che , Lee Ming-Ching , Huang Sheng-Yang , Chou Chia-Man , Yang Hui-Wen , Chen I-Chieh
Published: February 2024

Esophageal cancer shares strong associations with oropharyngeal and hypopharyngeal cancers, primarily due to shared risk factors like excessive tobacco and alcohol use. This retrospective study at Taichung Veterans General Hospital involved 54,692 participants, including 385 with squamous cell carcinoma (SCC) of the esophagus, oropharynx, or hypopharynx. Using a polygenic risk score (PRS) derived from 8353 single-nucleotide polymorphisms, researchers aimed to assess its correlation with cancer incidence and prognosis. The study found a 1.83-fold higher risk of esophageal, oropharyngeal, and hypopharyngeal SCCs in participants with a high PRS (Q4) compared to the low-PRS group (Q1). Esophageal cancer risk demonstrated a significant positive association with the PRS, as did hypopharyngeal cancer. Clinical parameters and staging showed limited associations with PRS quartiles, and the PRS did not significantly impact recurrence or mortality rates. The research highlighted that a higher PRS is linked to increased susceptibility to esophageal and hypopharyngeal cancer. Notably, a specific polygenic risk score, PGS001087, exhibited a discernible association with SCC risk, particularly in specific subtypes and advanced disease stages. However, it was not significantly linked to clinical cancer staging, emphasizing the multifactorial nature of cancer development. This hospital study reveals that a higher PRS correlates with increased susceptibility to esophageal and hypopharyngeal cancers. Notably, PGS001087 shows a discernible association with SCC risk in specific subtypes and advanced stages, although not significantly linked to clinical cancer staging. These findings enhance our understanding of genetic factors in upper aerodigestive tract cancers, particularly esophageal SCC, guiding future research and risk assessment strategies.
Regulating Lattice Oxygen on the Surfaces of Porous Single-Crystalline NiO for Stabilized and Enhanced CO Oxidation
Authors: Wen Xin , Xie Kui
Published: February 2024

The long-range ordered lattice structure and interconnected porous microstructure of porous single crystals (PSCs) provide structural regularity and connectivity in remote electron movement to stabilize oxygen vacancies and activate lattice oxygen linked to surface active sites. In this work, we prepare NiO powder, single-crystal (SC) NiO, and PSC NiO. NiO contains a significant amount of oxygen vacancies. We find that the structure of porous NiO can create more oxygen vacancies. We load Pt onto these NiO crystals by atomic layer deposition (ALD) to activate lattice oxygen on definite NiO surfaces. The results show that Pt-loaded NiO effectively exhibits CO oxidation performance, in which Pt-loaded PSC NiO completely oxidizes CO at 65 °C. With 1% CO fully adsorbed, the density of activate lattice oxygen becomes an essential factor affecting performance. PSC NiO with deposited Pt clusters exhibited stable CO oxidation catalysis when run in air at ~65 °C for 300 h.
Taming Microglia in Alzheimer’s Disease: Exploring Potential Implications of Choline Alphoscerate via α7 nAChR Modulation
Authors: Cantone Anna Flavia , Burgaletto Chiara , Di Benedetto Giulia , Pannaccione Anna , Secondo Agnese , Bellanca Carlo Maria , Augello Egle , Munafò Antonio , Tarro Paola , Bernardini Renato , Cantarella Giuseppina
Published: February 2024

Alzheimer’s disease (AD), marked by cognitive impairment, predominantly affects the brain regions regulated by cholinergic innervation, such as the cerebral cortex and hippocampus. Cholinergic dysfunction, a key contributor to age-related cognitive decline, has spurred investigations into potential therapeutic interventions. We have previously shown that choline alphoscerate (α-GPC), a cholinergic neurotransmission-enhancing agent, protects from Aβ-mediated neurotoxicity. Herein, we investigated the effects of α-GPC on the microglial phenotype in response to Aβ via modulation of the nicotinic alpha-7 acetylcholine receptor (α7 nAChR). BV2 microglial cells were pre-treated for 1 h with α-GPC and were treated for 24, 48, and 72 h with Aβ1–42 and/or α-BTX, a selective α7nAchR antagonist. Fluorescent immunocytochemistry and Western blot analysis showed that α-GPC was able to antagonize Aβ-induced inflammatory effects. Of note, α-GPC exerted its anti-inflammatory effect by directly activating the α7nAChR receptor, as suggested by the induction of an increase in [Ca2+]i and Ach-like currents. Considering that cholinergic transmission appears crucial in regulating the inflammatory profiles of glial cells, its modulation emerges as a potential pharmaco-therapeutic target to improve outcomes in inflammatory neurodegenerative disorders, such as AD.
A Bird’s-Eye View of Chromosomic Evolution in the Class Aves
Authors: O’Connor Rebecca E. , Kretschmer Rafael , Romanov Michael N. , Griffin Darren K.
Published: February 2024

Birds (Aves) are the most speciose of terrestrial vertebrates, displaying Class-specific characteristics yet incredible external phenotypic diversity. Critical to agriculture and as model organisms, birds have adapted to many habitats. The only extant examples of dinosaurs, birds emerged ~150 mya and >10% are currently threatened with extinction. This review is a comprehensive overview of avian genome (“chromosomic”) organization research based mostly on chromosome painting and BAC-based studies. We discuss traditional and contemporary tools for reliably generating chromosome-level assemblies and analyzing multiple species at a higher resolution and wider phylogenetic distance than previously possible. These results permit more detailed investigations into inter- and intrachromosomal rearrangements, providing unique insights into evolution and speciation mechanisms. The ‘signature’ avian karyotype likely arose ~250 mya and remained largely unchanged in most groups including extinct dinosaurs. Exceptions include Psittaciformes, Falconiformes, Caprimulgiformes, Cuculiformes, Suliformes, occasional Passeriformes, Ciconiiformes, and Pelecaniformes. The reasons for this remarkable conservation may be the greater diploid chromosome number generating variation (the driver of natural selection) through a greater possible combination of gametes and/or an increase in recombination rate. A deeper understanding of avian genomic structure permits the exploration of fundamental biological questions pertaining to the role of evolutionary breakpoint regions and homologous synteny blocks.
Adipose Tissue Macrophage Polarization Is Altered during Recovery after Exercise: A Large-Scale Flow Cytometric Study
Authors: Baek Kyung-Wan , Kim Ji Hyun , Yu Hak Sun , Kim Ji-Seok
Published: February 2024

We performed a large-scale flow cytometric analysis to determine whether M1 macrophage (M1Ø) and M2 macrophage (M2Ø) polarization in white adipose tissue (WAT) was altered immediately after exercise. Additionally, we comprehensively investigated the effects of obesity, exercise intensity, and recovery time on macrophage polarization in WAT. A single exercise bout of various intensities (ND, non-exercise control; -LIE, low-intensity exercise; -MIE, mid-intensity exercise; -HIE, high-intensity exercise) was performed by normal mice (ND) and obese mice (HFD). To confirm differences in M1Ø/M2Ø polarization in WAT based on the recovery time after a single exercise bout, WAT was acquired at 2 h, 24 h, and 48 h after exercise (total n = 168, 7 mice × 4 groups × 2 diets × 3 recovery time). The harvested WAT was immediately analyzed by flow cytometry, and macrophages were fluorescently labeled using F4/80, as well as M1Ø with CD11c and M2Øs with CD206. After a single bout of exercise, the M2Ø/M1Ø polarization ratio of WAT increases in both normal and obese mice, but differences vary depending on recovery time and intensity. Regardless of obesity, our findings showed that there could be a transient increase in M1Ø in WAT over a short recovery time (24 h) post-exercise (in ND-MIE, ND-HIE, and HFD-HIE). Furthermore, it was observed that the greater the exercise intensity in obese mice, the more effective the induction of M2Ø polarization immediately after exercise, as well as the maintenance of high M2Ø polarization, even after a prolonged recovery time.
Seasonal Stability Assessment of Reference Genes for Quantitative Real-Time Polymerase Chain Reaction Normalization in Bombus terrestris
Authors: Sankar Kathannan , Lee Kyeong-Yong , Kwak Kyu-Won , Lee Su-Jin , Lee Young-Bo
Published: February 2024

Bumblebees (B. terrestris) play a crucial role as highly efficient biological agents in commercial pollination. Understanding the molecular mechanisms governing their adaptation to diverse seasonal environments may pave the way for effective management strategies in the future. With the burgeoning advancement in post-genetic studies focusing on B. terrestris, there is a critical need to normalize quantitative real-time PCR (qRT-PCR) data using suitable reference genes. To address this necessity, we employed RefFinder, a software-based tool, to assess the suitability of several candidate endogenous control genes, including actin (ACT), arginine kinase (AK), elongation factor 1 alpha (EF1), glyceraldehyde-3-phosphate (GAPDH), phospholipase (PLA2), and ribosomal proteins (S18, S28). These genes were evaluated for their efficacy as biological endogenous controls by examining their expression patterns across various environmental conditions corresponding to different seasons (Spring, Summer, Autumn, Winter) and tissues (ovary, fat body, thorax, head) in bumblebees. Moreover, the study investigated the significance of selecting appropriate reference genes for three key genes involved in the juvenile hormone (JH) signaling pathways: Krüppel homolog 1 (Kr-h1), methyl farnesoate epoxidase (MFE), and Vitellogenin (Vg). Our research identifies specific genes suitable for normalization in B. terrestris, thereby offering valuable insights into gene expression and functional metabolic genetics under varying seasonal conditions. This catalog of reference genes will serve as a valuable resource for future research endeavors.
Relationship between Mental Disorders, Smoking or Alcoholism and Benign Prostate Disease
Authors: Chantada-Tirado Paloma , Chantada-Abal Venancio , Cózar-Ortiz José-David , Chantada-Tirado Cristina , Cózar-Olmo José-Manuel , Esteban-Fuertes Manuel , Alvarez-Ossorio-Rodal Andrea , Flores-Fraile Javier , Márquez-Sánchez Magaly-Teresa , Padilla-Fernández Bárbara-Yolanda , Lorenzo-Gómez María-Fernanda
Published: February 2024

Introduction: Mental disorders, smoking, or alcoholism and benign prostate disease are highly prevalent in men. Aims: To identify the relationship between mental disorders, smoking, or alcoholism and benign prostate disease. Methodology: A prospective multicenter study that evaluated prostate health status in 558 men from the community. Groups: GP—men who request a prostate health examination and whose medical history includes a mental disorder, smoking, or alcoholism prior to a diagnosis of benign prostate disease; GU—men who request a prostate health examination and whose medical history includes a benign prostate disease prior to a diagnosis of mental disorder, smoking, or alcoholism. Variables: age, body mass index (BMI), prostate specific antigen (PSA), follow-up of the mental disorder, smoking or alcoholism, time elapsed between urological diagnosis and the mental disorder, smoking or alcoholism diagnosis, status of the urological disease (cured or not cured), concomitant diseases, surgical history, and concomitant treatments. Descriptive statistics, Student’s t-test, Chi2, multivariate analysis. Results: There were no mental disorders, smoking, or alcoholism in 51.97% of men. Anxiety, smoking, major depressive disorder, pathological insomnia, psychosis, and alcoholism were identified in 19.71%, 13.26%, 5.73%, 4.30%, 2.87%, and 2.15% of individuals, respectively. Nonbacterial prostatitis (31.54%), urinary tract infection (other than prostatitis, 24.37%), prostatic intraepithelial neoplasia (13.98%), and prostatodynia (1.43%) were prostate diseases. Unresolved symptomatic benign prostate disease was associated with anxiety, depression, and psychosis (p = 0.002). Smoking was the disorder that men managed to eliminate most frequently. The dominant disorder in patients with symptomatic benign prostatic disease was alcoholism (p = 0.006). Conclusions: Unresolved symptomatic benign prostatic disease is associated with anxiety, depression, and psychosis. Alcoholism is associated with a worse prognosis in the follow-up of symptomatic benign prostatic disease.
Mercury Biogeochemistry and Biomagnification in the Mediterranean Sea: Current Knowledge and Future Prospects in the Context of Climate Change
Authors: Bargagli Roberto , Rota Emilia
Published: February 2024

In the 1970s, the discovery of much higher mercury (Hg) concentrations in Mediterranean fish than in related species of the same size from the Atlantic Ocean raised serious concerns about the possible health effects of neurotoxic monomethylmercury (MMHg) on end consumers. After 50 years, the cycling and fluxes of the different chemical forms of the metal between air, land, and marine environments are still not well defined. However, current knowledge indicates that the anomalous Hg accumulation in Mediterranean organisms is mainly due to the re-mineralization of organic material, which favors the activity of methylating microorganisms and increases MMHg concentrations in low-oxygen waters. The compound is efficiently bio-concentrated by very small phytoplankton cells, which develop in Mediterranean oligotrophic and phosphorous-limited waters and are then transferred to grazing zooplankton. The enhanced bioavailability of MMHg together with the slow growth of organisms and more complex and longer Mediterranean food webs could be responsible for its anomalous accumulation in tuna and other long-lived predatory species. The Mediterranean Sea is a “hotspot” of climate change and has a rich biodiversity, and the increasing temperature, salinity, acidification, and stratification of seawater will likely reduce primary production and change the composition of plankton communities. These changes will likely affect the accumulation of MMHg at lower trophic levels and the biomagnification of its concentrations along the food web; however, changes are difficult to predict. The increased evasion of gaseous elemental mercury (Hg°) from warming surface waters and lower primary productivity could decrease the Hg availability for biotic (and possibly abiotic) methylation processes, but lower oxygen concentrations in deep waters, more complex food webs, and the reduced growth of top predators could increase their MMHg content. Despite uncertainties, in Mediterranean regions historically affected by Hg inputs from anthropogenic and geogenic sources, such as those in the northwestern Mediterranean and the northern Adriatic Sea, rising seawater levels, river flooding, and storms will likely favor the mobilization of Hg and organic matter and will likely maintain high Hg bioaccumulation rates for a long time. Long-term studies will, therefore, be necessary to evaluate the impact of climate change on continental Hg inputs in the Mediterranean basin, on air–sea exchanges, on possible changes in the composition of biotic communities, and on MMHg formation and its biomagnification along food webs. In this context, to safeguard the health of heavy consumers of local seafood, it appears necessary to develop information campaigns, promote initiatives for the consumption of marine organisms at lower trophic levels, and organize large-scale surveys of Hg accumulation in the hair or urine of the most exposed population groups.
Efficacy and Skin Microbiome Modulation Effects of a Fixed-Concentration Combination of Benzoyl Peroxide 4% Plus Niacinamide 4% in a Film-Forming Cream in Subjects with Mild-to-Moderate Acne: A Non-Sponsored, Prospective, Assessor-Blinded, Pilot Trial
Authors: Puviani Mario , Eisendle Klaus
Published: February 2024

Benzoyl peroxide (BPO) and niacinamide (Niac) are commonly used alone or in combination with other molecules in the treatment of acne. BPO and Niac in the same product could exert complementary and synergistic effects improving efficacy in acne. Furthermore, Niac could reduce some side effects of BPO, such as skin irritation and erythema. A film-forming cream product containing BPO 4% and Niac 4%, vehiculated in a mixture of polyvinyl alcohol (PVA) and perfluoropolyether (PFPE) has been recently developed (B-N cream). A 28-day, assessor-blinded pilot trial was conducted to evaluate the clinical efficacy, skin tolerability, sebum production, skin redness, and skin microbiome modulation of B-N cream in subjects with mild to moderate acne of the face. Twenty-two adult men and women with mild-to-moderate acne of the face participated in this trial, after their written informed consent. B-N cream was applied once daily in the evening. The use of B-N cream was associated with a statistically significant decrease in acne lesions after treatment in comparison with baseline (non-inflammatory lesions: −40%; inflammatory lesions: −43% and total lesions: −41%). The use of the cream was not associated with a skin barrier function alteration. The skin redness score (−11%) and the sebum production (−42%) were significantly reduced after treatment. The use of B-N cream determined a reduction in the relative abundance of the Actinobacteria phylum (Corynebacterium and Propionibacterium, from 56% to 47%) and a significant increase in the abundance of Bacteroidetes phylum (from 3% to 5.2%). In addition, the product was well tolerated. In conclusion, this film-forming, medical device cream was effective in subjects with mild-to-moderate acne, reducing acne lesions and sebum production without altering skin barrier function, offering good skin tolerability. Furthermore, this product exerts positive skin microbiome modulation effects.