bluuebunny/crossref_metadata_2025 huggingface dataset:
Dissecting the Regulation of Arachidonic Acid Metabolites by Uncaria rhynchophylla (Miq). Miq. in Spontaneously Hypertensive Rats and the Predictive Target sEH in the Anti-Hypertensive Effect Based on Metabolomics and Molecular Docking
Authors: Gao Lei , Kong Xinqin , Wu Wenyong , Feng Zijin , Zhi Haijuan , Zhang Zijia , Long Huali , Lei Min , Hou Jinjun , Wu Wanying , Guo De-an
Published: June 2022

Uncariarhynchophylla (Miq). Miq. (UR), as a traditional Chinese medicine, was employed in treating hypertension as a safe and effective therapy. The pharmacological properties of UR have characteristics of multiple biological targets and multiple functional pathways. Hypertension is related to impaired metabolic homeostasis and is especially associated with the abnormal regulation of arachidonic acid metabolites, the classical cardiovascular active compounds. This study aimed to examine the anti-hypertensive effect of UR extract (URE) and its regulating role in differential metabolic pathways. The results showed that daily administration of URE at a dose of 4 g crude drug/kg orally could exert hypotensive effects on spontaneously hypertensive rats (SHRs) for 8 weeks. Non-targeted metabolomics analysis of the plasma samples suggested that the anti-hypertension effect of URE in SHRs was associated with the reorganization of the perturbed metabolic network, such as the pathways of glycerophospholipid metabolism, linoleic acid metabolism, and arachidonic acid metabolism. For the targeted metabolomics, twenty-eight arachidonic acid metabolites in SHRs were quantitatively analyzed for the first time based on ultra-high performance liquid chromatography-tandem mass spectrometry method after URE administration. URE restored the functions of these cardiovascular active compounds and rebalanced the dynamics of arachidonic acid metabolic flux. Among them, the inhibition of soluble epoxide hydrolase (sEH) enzyme activity and up-regulation of vasodilators epoxyeicosatrienoic acids (EETs) were identified as contributors to the anti-hypertension effect of URE on SHRs, and sEH represented an attractive and promising drug-binding target of URE. With the molecular docking approach, 13 potential anti-hypertension ingredients as well as sEH inhibitors were discovered, which were worthy of further investigation and verification in future studies.
Hydrogen Sulfide-Induced Vasodilation: The Involvement of Vascular Potassium Channels
Authors: Liu Xiao-Yu , Qian Ling-Ling , Wang Ru-Xing
Published: June 2022

Hydrogen sulfide (H2S) has been highlighted as an important gasotransmitter in mammals. A growing number of studies have indicated that H2S plays a key role in the pathophysiology of vascular diseases and physiological vascular homeostasis. Alteration in H2S biogenesis has been reported in a variety of vascular diseases and H2S supplementation exerts effects of vasodilation. Accumulating evidence has shown vascular potassium channels activation is involved in H2S-induced vasodilation. This review aimed to summarize and discuss the role of H2S in the regulation of vascular tone, especially by interaction with different vascular potassium channels and the underlying mechanisms.
A Twitter-Based Economic Policy Uncertainty Index: Expert Opinion and Financial Market Dynamics in an Emerging Market Economy
Authors: Yeşiltaş Sevcan , Şen Anıl , Arslan Beyza , Altuğ Sumru
Published: June 2022

In this paper, we construct a Twitter-based high-frequency Economic Policy Uncertainty (TEPU) index built on a select set of Twitter user accounts whose tweets are considered to reflect expert opinion on the topic. We study the relationship between the TEPU index and a set of key financial indicators for tracking financial developments in Turkey over the sample period 2013–2021. Based on the results from a vector autoregressive analysis, we find evidence that changes in expert opinion described by fluctuations in the TEPU index interact with fluctuations in financial indicators such as the exchange rate and the stock market index to capture information about high frequency events during our sample period. Second, fluctuations in the TEPU index emerge as a key indicator that helps to predict the country risk premium measured by the CDS spread. We also find evidence that the conditional volatility of the different series reflects salient events that occurred over our sample period.
Fibre-Optic Dosimetry for MRI-LINACs: A Mini-Review
Authors: Madden Levi , Holloway Lois , Rosenfeld Anatoly , Li Enbang
Published: June 2022

For the past few decades, fibre-optic dosimeters (FODs) have been a focus of research for dosimetry with LINACs, owing to a unique set of advantageous qualities: compact dosimeter sizes, an all optical composition (i.e. no wires or electronics around their sensitive volume), real-time response proportional to the absorbed dose-rate in their sensitive volumes and direct water equivalence. Such a set of qualities makes FODs “near-correctionless” for dosimetry with LINACs, such that they have been recommended as in vivo dosimeters and small field dosimeters. Further, their scintillation and luminescence response mechanisms are not affected by magnetic fields. Given this set of qualities, FODs are attractive candidates for dosimetry with MRI-LINACs. This mini-review aims to provide an overview of FODs to the wider medical physics community, and present the current challenges and opportunities for FODs given previous investigations into MRI-LINAC dosimetry.
Reciprocal Space Study of Brownian Yet Non-Gaussian Diffusion of Small Tracers in a Hard-Sphere Glass
Authors: Brizioli Matteo , Sentjabrskaja Tatjana , Egelhaaf Stefan U. , Laurati Marco , Cerbino Roberto , Giavazzi Fabio
Published: June 2022

The simultaneous presence of normal (Brownian) diffusion and non-Gaussian statistics of particle displacements has been identified as a recurring motif for a broad spectrum of physical and biological systems. While not yet fully understood, it is generally accepted that a key ingredient for observing this Brownian yet non-Gaussian (BNG) diffusion is that the environment hosting the particles appears stationary and homogenous on the small length and time scales, while displaying significant fluctuations on larger distances and/or longer time scales. To date, most of the experimental studies on systems displaying BNG diffusion have been performed in direct space, usually via a combination of optical microscopy and particle tracking to quantify the particle’s self-diffusion. Here, we demonstrate that a reciprocal space analysis of the density fluctuations caused by the particle motion as a function of the wave vector enables the investigation of BNG diffusion in situations where single-particle tracking is impossible. To accomplish this aim, we use confocal differential dynamic microscopy (ConDDM) to study the BNG dynamics of diluted sub-resolution tracers diffusing in a glassy matrix of larger hard spheres. We first elucidate the nontrivial connection between the tracer self-diffusion and collective relaxation of the resulting density fluctuations. We find that the experimentally determined intermediate scattering functions are in excellent agreement with the recent predictions of a “diffusing diffusivity” model of BNG diffusion, whose analytical predictions are available only in reciprocal space. Our results show that studying BNG diffusion in reciprocal space can be an invaluable strategy to access the fast, anomalous dynamics occurring at very small scales in crowded environments.
Optimization of Whole Mount RNA Multiplexed in situ Hybridization Chain Reaction With Immunohistochemistry, Clearing and Imaging to Visualize Octopus Embryonic Neurogenesis
Authors: Elagoz Ali M. , Styfhals Ruth , Maccuro Sofia , Masin Luca , Moons Lieve , Seuntjens Eve
Published: June 2022

Gene expression analysis has been instrumental to understand the function of key factors during embryonic development of many species. Marker analysis is also used as a tool to investigate organ functioning and disease progression. As these processes happen in three dimensions, the development of technologies that enable detection of gene expression in the whole organ or embryo is essential. Here, we describe an optimized protocol of whole mount multiplexed RNA in situ hybridization chain reaction version 3.0 (HCR v3.0) in combination with immunohistochemistry (IHC), followed by fructose-glycerol clearing and light sheet fluorescence microscopy (LSFM) imaging on Octopus vulgaris embryos. We developed a code to automate probe design which can be applied for designing HCR v3.0 type probe pairs for fluorescent in situ mRNA visualization. As proof of concept, neuronal (Ov-elav) and glial (Ov-apolpp) markers were used for multiplexed HCR v3.0. Neural progenitor (Ov-ascl1) and precursor (Ov-neuroD) markers were combined with immunostaining for phosphorylated-histone H3, a marker for mitosis. After comparing several tissue clearing methods, fructose-glycerol clearing was found optimal in preserving the fluorescent signal of HCR v3.0. The expression that was observed in whole mount octopus embryos matched with the previous expression data gathered from paraffin-embedded transverse sections. Three-dimensional reconstruction revealed additional spatial organization that had not been discovered using two-dimensional methods.
Pharmacological Melanocortin 5 Receptor Activation Attenuates Glomerular Injury and Proteinuria in Rats With Puromycin Aminonucleoside Nephrosis
Authors: Chen Bohan , Alam Zubia , Ge Yan , Dworkin Lance , Gong Rujun
Published: June 2022

Clinical evidence indicates that the melanocortin peptide ACTH is effective in inducing remission of nephrotic glomerulopathies like minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS), including those resistant to steroids. This suggests that a steroid-independent melancortinergic mechanism may contribute. However, the type of melanocortin receptor (MCR) that conveys this beneficial effect as well as the underlying mechanisms remain controversial. Burgeoning evidence suggests that MC5R is expressed in glomeruli and may be involved in glomerular pathobiology. This study aims to test the effectiveness of a novel highly selective MC5R agonist (MC5R-A) in puromycin aminonucleoside (PAN) nephrosis. Upon PAN injury, rats developed evident proteinuria on day 5, denoting an established nephrotic glomerulopathy. Following vehicle treatment, proteinuria continued to persist on day 14 with prominent histologic signs of podocytopathy, marked by ultrastructural glomerular lesions, including extensive podocyte foot process effacement. Concomitantly, there was loss of podocyte homeostatic markers, such as synaptopodin and podocin, and de novo expression of the podocyte injury marker desmin. Treatment with MC5R-A attenuated urine protein excretion and mitigated the loss of podocyte marker proteins, resulting in improved podocyte ultrastructural changes. In vitro in cultured podocytes, MC5R-A prevented the PAN-induced disruption of actin cytoskeleton integrity and apoptosis. MC5R-A treatment in PAN-injured podocytes also reinstated inhibitory phosphorylation and thus averted hyperactivity of GSK3β, a convergent point of multiple podocytopathic pathways. Collectively, pharmacologic activation of MC5R by using the highly selective small-molecule agonist is likely a promising therapeutic strategy to improve proteinuria and glomerular injury in protenuric nephropathies.
The Chemerin-CMKLR1 Axis is Functionally important for Central Regulation of Energy Homeostasis
Authors: Yun Haesung , Dumbell Rebecca , Hanna Katie , Bowen Junior , McLean Samantha L. , Kantamneni Sriharsha , Pors Klaus , Wu Qing-Feng , Helfer Gisela
Published: June 2022

Chemerin is an adipokine involved in inflammation, adipogenesis, angiogenesis and energy metabolism, and has been hypothesized as a link between obesity and type II diabetes. In humans affected by obesity, chemerin gene expression in peripheral tissues and circulating levels are elevated. In mice, plasma levels of chemerin are upregulated by high-fat feeding and gain and loss of function studies show an association of chemerin with body weight, food intake and glucose homeostasis. Therefore, chemerin is an important blood-borne mediator that, amongst its other functions, controls appetite and body weight. Almost all studies of chemerin to date have focused on its release from adipose tissue and its effects on peripheral tissues with the central effects largely overlooked. To demonstrate a central role of chemerin, we manipulated chemerin signaling in the hypothalamus, a brain region associated with appetite regulation, using pharmacological and genetic manipulation approaches. Firstly, the selective chemerin receptor CMKLR1 antagonist α-NETA was administered i.c.v. to rats to test for an acute physiological effect. Secondly, we designed a short-hairpin-RNA (shRNA) lentivirus construct targeting expression of CMKLR1. This shRNA construct, or a control construct was injected bilaterally into the arcuate nucleus of male Sprague Dawley rats on high-fat diet (45%). After surgery, rats were maintained on high-fat diet for 2 weeks and then switched to chow diet for a further 2 weeks. We found a significant weight loss acutely and inhibition of weight gain chronically. This difference became apparent after diet switch in arcuate nucleus-CMKLR1 knockdown rats. This was not accompanied by a difference in blood glucose levels. Interestingly, appetite-regulating neuropeptides remained unaltered, however, we found a significant reduction of the inflammatory marker TNF-α suggesting reduced expression of CMKLR1 protects from high-fat diet induced neuroinflammation. In white and brown adipose tissue, mRNA expression of chemerin, its receptors and markers of adipogenesis, lipogenesis and brown adipocyte activation remained unchanged confirming that the effects are driven by the brain. Our behavioral analyses suggest that knockdown of CMKLR1 had an impact on object recognition. Our data demonstrate that CMKLR1 is functionally important for the central effects of chemerin on body weight regulation and neuroinflammation.
Early and Late-Phase 24 h Responses of Stored Red Blood Cells to Recipient-Mimicking Conditions
Authors: Tzounakas Vassilis L. , Anastasiadi Alkmini T. , Karadimas Dimitrios G. , Velentzas Athanassios D. , Anastasopoulou Violetta I. , Papageorgiou Effie G. , Stamoulis Konstantinos , Papassideri Issidora S. , Kriebardis Anastasios G. , Antonelou Marianna H.
Published: June 2022

The 24-hour (24 h) post-transfusion survival of donor red blood cells (RBCs) is an important marker of transfusion efficacy. Nonetheless, within that period, donated RBCs may encounter challenges able to evoke rapid stress-responses. The aim of the present study was to assess the effect of exposure to plasma and body temperature upon stored RBCs under recipient-mimicking conditions in vitro from the first hours “post-transfusion” up to 24 h. For this purpose, packed RBCs from seven leukoreduced CPD/SAGM units were reconstituted with plasma of twenty-seven healthy individuals and incubated for 24 h at 37oC. Three units were additionally used to examine stress-responses in 3-hour intervals post mixing with plasma (n = 5) until 24 h. All experiments were performed in shortly-, medium-, and long-stored RBCs. Hemolysis, redox, morphology, membrane protein binding and vesiculation parameters were assessed. Even though spontaneous hemolysis was minimal post-reconstitution, it presented a time-dependent increase. A similar time-course profile was evident for the concentration of procoagulant extracellular vesicles and the osmotic fragility (shortly-stored RBCs). On the contrary, mechanical fragility and reactive oxygen species accumulation were characterized by increases in medium-stored RBCs, evident even from the first hours in the recipient-mimicking environment. Finally, exposure to plasma resulted in rapid improvement of morphology, especially in medium-stored RBCs. Overall, some RBC properties vary significantly during the first 24 h post-mixing, at levels different from both the storage ones and the standard end-of-24 h. Such findings may be useful for understanding the performance of RBCs and their possible clinical effects −especially on susceptible recipients− during the first hours post-transfusion.
Network Representation Learning With Community Awareness and Its Applications in Brain Networks
Authors: Shi Min , Qu Bo , Li Xiang , Li Cong
Published: June 2022

Previously network representation learning methods mainly focus on exploring the microscopic structure,i.e., the pairwise relationship or similarity between nodes. However, the mesoscopic structure,i.e., community structure, an essential property in real networks, has not been thoroughly studied in the network representation learning. We here propose a deep attributed network representation learning with community awareness (DANRL-CA) framework. Specifically, we design a neighborhood enhancement autoencoder module to capture the 2-step relations between node pairs. To explore the multi-step relations, we construct a community-aware skip-gram module based on the encoder. We introduce two variants of DANRL-CA, namely, DANRL-CA-AM and DANRL-CA-CSM, which incorporate the community information and attribute semantics into node neighbors with different methods. We compare two variant models with the state-of-the-art methods on four datasets for node classification and link prediction. Especially, we apply our models on a brain network. The superiority indicates the scalability and effectiveness of our method on various networks. Compared with DANRL-CA-AM, DANRL-CA-CSM can more flexibly coordinate the role of node attributes and community information in the process of network representation learning, and shows superiority in the networks with sparse topological structure and node attributes.
Effects of Acute Exposure to Polystyrene Nanoplastics on the Channel Catfish Larvae: Insights From Energy Metabolism and Transcriptomic Analysis
Authors: Jiang Qichen , Chen Xiaohui , Jiang Hucheng , Wang Minghua , Zhang Tongqing , Zhang Wenyi
Published: June 2022

Microplastics (nanoplastics) pollution has been a major ecological issue threatening global aquatic ecosystems. However, knowledge of the adverse effects of nanoplastics and the effects on freshwater ecosystems is still limited. To understand the impacts of nanoplastics on freshwater ecosystems, it is essential to reveal the physiological changes caused by nanoplastics in freshwater organisms, especially at their early life-history stages. In the present study, the larval channel catfish Ietalurus punetaus were exposed to gradient concentrations (0, 5, 10, 25, and 50 mg/L) of 75-nm polystyrene nanoplastics (PS-NPs) for 24 h or 48 h, and changes in contents of energy metabolites, metabolic enzyme activities and transcriptome were assessed. The results showed that glucose and triglyceride contents increased after 24 h of exposure to 10 or 25 mg/L of PS-NPs but decreased with increased concentrations or prolonged exposure duration. Activities of most metabolic enzymes analyzed decreased in the larvae after 48 h of exposure, especially in 25 or 50 mg/L of PS-NPs. These suggested that PS-NPs caused huge energy consumption and disturbed the energy metabolism in larval fish. Transcriptomic analysis showed that 48 h of exposure to 50 mg/L PS-NPs affected the expression of genes involved in protein digestion and induced response of proteasomes or heat shock proteins in the larval I. punetaus. The genes involved in peroxisome proliferator-activated receptors (PPAR) pathway and biosynthesis of amino acids were activated after the exposure. PS-NPs also depressed the expression of the genes involved in gonad development or muscle contraction in the larval I. punetaus. Overall, acute exposure to 75-nm PS-NPs disrupted the energy metabolism by consuming the energy reserves, and affected a series of molecular pathways which may further affect the development and survival of fish. This study provided the information about adverse effects of nanoplastics on the fish larvae and revealed the molecular pathways for the potential adverse outcomes.
Detection of Anomalous Grapevine Berries Using Variational Autoencoders
Authors: Miranda Miro , Zabawa Laura , Kicherer Anna , Strothmann Laurenz , Rascher Uwe , Roscher Ribana
Published: June 2022

Grapevine is one of the economically most important quality crops. The monitoring of the plant performance during the growth period is, therefore, important to ensure a high quality end-product. This includes the observation, detection, and respective reduction of unhealthy berries (physically damaged, or diseased). At harvest, it is not necessary to know the exact cause of the damage, but rather if the damage is apparent or not. Since a manual screening and selection before harvest is time-consuming and expensive, we propose an automatic, image-based machine learning approach, which can lead observers directly to anomalous areas without the need to monitor every plant manually. Specifically, we train a fully convolutional variational autoencoder with a feature perceptual loss on images with healthy berries only and consider image areas with deviations from this model as damaged berries. We use heatmaps which visualize the results of the trained neural network and, therefore, support the decision making for farmers. We compare our method against a convolutional autoencoder that was successfully applied to a similar task and show that our approach outperforms it.
Plant Growth-Promoting Rhizobacteria Alleviate High Salinity Impact on the Halophyte Suaeda fruticosa by Modulating Antioxidant Defense and Soil Biological Activity
Authors: Hidri Rabaa , Mahmoud Ouissal Metoui-Ben , Zorrig Walid , Mahmoudi Henda , Smaoui Abderrazak , Abdelly Chedly , Azcon Rosario , Debez Ahmed
Published: June 2022

Plant growth-promoting rhizobacteria (PGPR) are considered as bio-ameliorators that confer better salt resistance to host plants while improving soil biological activity. Despite their importance, data about the likely synergisms between PGPR and halophytes in their native environments are scarce. The objective of this study was to assess the effect of PGPR (Glutamicibactersp. andPseudomonassp.) inoculation on biomass, nutrient uptake, and antioxidant enzymes ofSuaeda fruticosa, an obligate halophyte native in salt marshes and arid areas in Tunisia. Besides, the activity of rhizospheric soil enzyme activities upon plant inoculation was determined. Plants were grown in pots filled with soil and irrigated with 600 mM NaCl for 1 month. Inoculation (either withPseudomonassp. orGlutamicibactersp.) resulted in significantly higher shoot dry weight and less accumulation of Na+and Cl–in shoots of salt-treated plants.Glutamicibactersp. inoculation significantly reduced malondialdehyde (MDA) concentration, while increasing the activity of antioxidant enzymes (superoxide dismutase; catalase; ascorbate peroxidase; and glutathione reductase) by up to 100%. This provides strong arguments in favor of a boosting effect of this strain onS. fruticosachallenged with high salinity.Pseudomonassp. inoculation increased shoot K+and Ca2+content and lowered shoot MDA concentration. Regarding the soil biological activity,Pseudomonassp. significantly enhanced the activities of three rhizospheric soil enzymes (urease, ß-glucosidase, and dehydrogenase) as compared to their respective non-inoculated saline treatment. Hence,Pseudomonassp. could have a great potential to be used as bio-inoculants in order to improve plant growth and soil nutrient uptake under salt stress. Indole-3-acetic acid concentration in the soil increased in both bacterial treatments under saline conditions, especially withGlutamicibactersp. (up to +214%). As a whole,Glutamicibactersp. andPseudomonassp. strains are promising candidates as part of biological solutions aiming at the phytoremediation and reclamation of saline-degraded areas.
Water Availability, Soil Characteristics, and Confounding Effects on the Patterns of Biocrust Diversity in the Desert Regions of Northern China
Authors: Sun Jingyao , Li Xinrong
Published: June 2022

The species diversity of biocrusts is an important community characteristic in determining their multiple ecosystem functions. Hence, understanding the diversity patterns of biocrusts and their environmental drivers is of fundamental importance. However, explain variables often correlated with each other; thus, the confounding effects among them may arise and result in spurious causal relationships and biased ecological inferences. In this study, we investigated the richness of three biocrust-forming components (mosses, lichens, and cyanobacteria–algae) and their environmental variables across six desert regions of northern China. A comparison between conventional redundancy analysis (RDA) and structural equation model (SEM) was conducted to study the environmental driver-richness relationship and the confounding effects. Our results showed that three latent variables related to water availability, soil texture, and soil salinity and sodicity, could account for the main environmental variations and explain the diversity patterns of biocrusts at the intracontinental scale. Water availability was positively and negatively related to the richness of mosses and cyanobacteria–algae, respectively, while soil texture was positively related to the richness of lichens. In addition, environmental variables confounded with each other caused distinct driver-richness relationships between results of RDA and SEM. Therefore, we suggest that future multivariable studies should utilize path analysis in conjunction with conventional canonical ordination to facilitate more rigorous ecological inferences.
Resveratrol Oligomers, Plant-Produced Natural Products With Anti-virulence and Plant Immune-Priming Roles
Authors: Kang Ji Eun , Yoo Nayeon , Jeon Byeong Jun , Kim Beom Seok , Chung Eui-Hwan
Published: June 2022

Antibiotic resistance has become increasingly prevalent in the environment. Many alternative strategies have been proposed for the treatment and prevention of diverse diseases in agriculture. Among them, the modulation of bacterial virulence to bypass antibiotic resistance or boost plant innate immunity can be considered a promising drug target. Plant-produced natural products offer a broad spectrum of stereochemistry and a wide range of pharmacophores, providing a great diversity of biological activities. Here, we present a perspective on the putative role of plant-produced resveratrol oligomers as anti-virulence and plant-immune priming agents for efficient disease management. Resveratrol oligomers can decrease (1) bacterial motility directly and (2) indirectly by attenuating the bacterial type III secretion system (TT3S). They induce enhanced local immune responses mediated by two-layered plant innate immunity, demonstrating (3) a putative plant immune priming role.
The Emerging Role of Proline in the Establishment and Functioning of Legume-Rhizobium Symbiosis
Authors: Sabbioni Giuseppe , Forlani Giuseppe
Published: June 2022

High levels of some enzymes involved in proline synthesis and utilization were early found in soybean nodules, and rhizobial knockout mutants were shown to be defective in inducing nodulation and/or fixing nitrogen, leading to postulate that this amino acid may represent a main substrate for energy transfer from the plant to the symbiont. However, inconsistent results were reported in other species, and several studies suggested that proline metabolism may play an essential role in the legume-Rhizobium symbiosis only under stress. Different mechanisms have been hypothesized to explain the beneficial effects of proline on nodule formation and bacteroid differentiation, yet none of them has been conclusively proven. Here, we summarize these findings, with special emphasis on the occurrence of a legume-specific isoform of δ1-pyrroline-5-carboxylate synthetase, the enzyme that catalyses the rate-limiting step in proline synthesis. Data are discussed in view of recent results connecting the regulation of both, the onset of nodulation and proline metabolism, to the redox status of the cell. Full comprehension of these aspects could open new perspectives to improve the adaptation of legumes to environmental stress.
Soil Inoculation With Beneficial Microbes Buffers Negative Drought Effects on Biomass, Nutrients, and Water Relations of Common Myrtle
Authors: Azizi Soghra , Tabari Masoud , Abad Ali Reza Fallah Nosrat , Ammer Christian , Guidi Lucia , Bader Martin K.-F.
Published: June 2022

Common myrtle (Myrtus communis L.) occurs in (semi-)arid areas of the Palearctic region where climate change, over-exploitation, and habitat destruction imperil its existence. The evergreen shrub is of great economic and ecological importance due to its pharmaceutical value, ornamental use, and its role in urban greening and habitat restoration initiatives. Under greenhouse conditions, we investigated the effect of soil inoculation with arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (PGPR) on biomass allocation, water relations, and nutritional status of drought-stressed myrtle seedlings. Single and dual AMF (Funneliformis mosseae and Rhizophagus irregularis) and PGPR (Pseudomonas fluorescens and P. putida) soil inoculations were applied to myrtle seedlings growing under different soil water regimes (100, 60, and 30% of field capacity) for 6 months using a full factorial, completely randomized design. AMF and PGPR treatments, especially dual inoculations, alleviated negative drought effects on biomass and morpho-physiological traits, except for water-use efficiency, which peaked under severe drought conditions. Under the greatest soil water deficit, dual inoculations promoted leaf biomass (104%–108%), root biomass (56%–73%), mesophyll conductance (58%), and relative water content (1.4-fold) compared to non-inoculated controls. Particularly, dual AMF and PGPR inoculations stimulated nutrient dynamics in roots (N: 138%–151%, P: 176%–181%, K: 112%–114%, Ca: 124%–136%, and Mg: 130%–140%) and leaves (N: 101%–107%, P: 143%–149%, K: 83%–84%, Ca: 98%–107%, and Mg: 102%–106%). Our findings highlight soil inoculations with beneficial microbes as a cost-effective way to produce highly drought resistant seedling stock which is vital for restoring natural myrtle habitats and for future-proofing myrtle crop systems.
RAD-Seq-Based High-Density Linkage Maps Construction and Quantitative Trait Loci Mapping of Flowering Time Trait in Alfalfa (Medicago sativa L.)
Authors: Jiang Xueqian , Yang Tianhui , Zhang Fan , Yang Xijiang , Yang Changfu , He Fei , Long Ruicai , Gao Ting , Jiang Yiwei , Yang Qingchuan , Wang Zhen , Kang Junmei
Published: June 2022

Alfalfa (Medicago sativa L.) is a perennial forage crop known as the “Queen of Forages.” To dissect the genetic mechanism of flowering time (FT) in alfalfa, high−density linkage maps were constructed for both parents of an F1 mapping population derived from a cross between Cangzhou (P1) and ZhongmuNO.1 (P2), consisting of 150 progenies. The FT showed a transgressive segregation pattern in the mapping population. A total of 13,773 single-nucleotide polymorphism markers was obtained by using restriction-site associated DNA sequencing and distributed on 64 linkage groups, with a total length of 3,780.49 and 4,113.45 cM and an average marker interval of 0.58 and 0.59 cM for P1 and P2 parent, respectively. Quantitative trait loci (QTL) analyses were performed using the least square means of each year as well as the best linear unbiased prediction values across 4 years. Sixteen QTLs for FT were detected for P1 and 22 QTLs for P2, accounting for 1.40–16.04% of FT variation. RNA-Seq analysis at three flowering stages identified 5,039, 7,058, and 7,996 genes that were differentially expressed between two parents, respectively. Based on QTL mapping, DEGs analysis, and functional annotation, seven candidate genes associated with flowering time were finally detected. This study discovered QTLs and candidate genes for alfalfa FT, making it a useful resource for breeding studies on this essential crop.
Evaluation of Fusarium Head Blight Resistance in 410 Chinese Wheat Cultivars Selected for Their Climate Conditions and Ecological Niche Using Natural Infection Across Three Distinct Experimental Sites
Authors: Yan Zhen , Chen Wanquan , van der Lee Theo , Waalwijk Cees , van Diepeningen Anne D. , Feng Jie , Zhang Hao , Liu Taiguo
Published: June 2022

Exploiting wheat cultivars with stable resistance to Fusarium Head blight (FHB) and toxin accumulation is a cost-effective and environmentally friendly strategy to reduce the risk of yield losses and contamination with mycotoxins. To facilitate the deployment of stable cultivar resistance, we evaluated FHB resistance and resistance to mycotoxin accumulation in 410 wheat lines bred by local breeders from four major wheat growing regions in China after natural infection at three distinct locations (Hefei, Yangzhou and Nanping). Significant differences in disease index were observed among the three locations. The disease indexes (DI’s) in Nanping were the highest, followed by Yangzhou and Hefei. The distribution of DI’s in Yangzhou showed the best discrimination of FHB resistance in cultivars. Growing region and cultivar had significant effect on DI and mycotoxins. Among the climate factors, relative humidity and rainfall were the key factors resulting in the severe disease. Even though most cultivars were still susceptible to FHB under the strongly conducive conditions applied, the ratio of resistant lines increased in the Upper region of the Yangtze River (UYR) and the Middle and Lower Region of the Yangtze River (MLYR) between 2015 and 2019. Deoxynivalenol (DON) was the dominant mycotoxin found in Hefei and Yangzhou, while NIV was predominant in Nanping. Disease indexes were significantly correlated with DON content in wheat grain.
Modification of Rhizosphere Microbial Communities: A Possible Mechanism of Plant Growth Promoting Rhizobacteria Enhancing Plant Growth and Fitness
Authors: Kong Zhaoyu , Liu Hongguang
Published: June 2022

Plant beneficial bacteria, defined as plant growth-promoting rhizobacteria (PGPR), play a crucial role in plants’ growth, stress tolerance and disease prevention. In association with the rhizosphere of plants, PGPR facilitate plant growth and development either directly or indirectly through multiple mechanisms, including increasing available mineral nutrients, moderating phytohormone levels and acting as biocontrol agents of phytopathogens. It is generally accepted that the effectiveness of PGPR inoculants is associated with their ability to colonize, survive and persist, as well as the complex network of interactions in the rhizosphere. Despite the promising plant growth promotion results commonly reported and mostly attributed to phytohormones or other organic compounds produced by PGPR inoculants, little information is available on the potential mechanisms underlying such positive effects via modifying rhizosphere microbial community and soil functionality. In this review, we overviewed the effects of PGPR inoculants on rhizosphere microbial ecology and soil function, hypothesizing that PGPR may indirectly promote plant growth and health via modifying the composition and functioning of rhizosphere microbial community, and highlighting the further directions for investigating the role of PGPR in rhizosphere from an ecological perspective.
Getting the Picture: Defining Race-Based Stereotypes in Politics
Authors: Bouchard Joanie
Published: June 2022

This article considers electoral inter-group dynamics in Quebec, Canada, by focusing on what White voters expect from political candidates of color. While significant work has been done on the use of political heuristics such as race or gender-based framing by the media, we do not know as much about the way voters interpret and use these stereotypes in a political context. In this article, we consider voters' interpretation of race-based cues using qualitative evidence gathered in six focus groups. First, we explore the content of stereotypes typically associated with politicians of color in the province. Second, this article provides an assessment of some of the ways in which race-based stereotypes are used to understand politics and evaluate politicians of color. We find that race-based stereotypes contribute to defining expectations regarding politicians' behavior. While voters may consciously choose to favor politicians of color, the perception of social distance between a marginalized candidate and them can also lead to negative cross-ethnic attitudes.
Critical Faculty and Peer Instructor Development: Core Components for Building Inclusive STEM Programs in Higher Education
Authors: von Vacano Claudia , Ruiz Michael , Starowicz Renee , Olojo Seyi , Moreno Luna Arlyn Y. , Muzzall Evan , Mendoza-Denton Rodolfo , Harding David J.
Published: June 2022

First-generation college students and those from ethnic groups such as African Americans, Latinx, Native Americans, or Indigenous Peoples in the United States are less likely to pursue STEM-related professions. How might we develop conceptual and methodological approaches to understand instructional differences between various undergraduate STEM programs that contribute to racial and social class disparities in psychological indicators of academic success such as learning orientations and engagement? Within social psychology, research has focused mainly on student-level mechanisms surrounding threat, motivation, and identity. A largely parallel literature in sociology, meanwhile, has taken a more institutional and critical approach to inequalities in STEM education, pointing to the macro level historical, cultural, and structural roots of those inequalities. In this paper, we bridge these two perspectives by focusing oncritical faculty and peer instructor developmentas targets for inclusive STEM education. These practices, especially when deployed together, have the potential to disrupt the unseen but powerful historical forces that perpetuate STEM inequalities, while also positively affecting student-level proximate factors, especially for historically marginalized students.
Enhancing Schools’ Development Activities on Inclusive Education Through In-service Training Course for School Teams: A Case Study
Authors: Kivirand Tiina , Leijen Äli , Lepp Liina
Published: June 2022

Most countries face the challenge of reconstructing their education systems to ensure equitable quality education for all children in inclusive settings. This challenge is also relevant in Estonia, the context of this study. A long-term in-service training course for school teams (school leaders, support specialists, and teachers) was developed and implemented in Estonia. The main goal of the training course was to develop attitudes, skills, and knowledge of school staff about the concept and meaning of inclusive education (IE) and the effective implementation through inclusive school development strategies. The aim of the current study was to find out how the in-service training course for school teams influences system-wide changes in the implementation of IE at the school level and what factors affect it. Purposeful sampling (two schools) was used, and the qualitative thematic case study research method was chosen to find answers to the research questions. Data were collected from school policy documents, homework assignments of the training course, semi-structured interviews in the middle and at the end of the training course, open-ended questionnaires at the end of the training course, and researcher diary. The results showed that the in-service training course for school teams enhanced cultural and structural changes at the school level. These changes were influenced by factors such as leadership, collaboration, commitment, and contribution of different parties, system-wide approach, resources, and external expertise. The implications of these findings are discussed further in the paper.
Mouth Movements as Possible Cues of Social Interest at Birth: New Evidences for Early Communicative Behaviors
Authors: Guellai Bahia , Streri Arlette
Published: June 2022

Previous studies evidenced that different interactive contexts modulate the visual attention of newborns. In the present study, we investigated newborns' motor feedback as an additional cue to neonates' expression of interest. Using videos of interactive faces and a familiarization-test procedure, three different groups of newborns were assigned to three different conditions (i.e., one condition with a talking face during familiarization and silently moving faces at test, silently moving/silently moving condition, or talking/static condition). Following studies on neonatal imitation, mouth movements were analyzed as indicators of social interest. We expected the occurrence of mouth movements in the newborns to differ according to different conditions: (a) whether or not the face in front of them was talking and (b) if the person had been already seen or was new. Results revealed that a talking face elicited more motor feedback from the newborns than a silent one and that there was no difference in front of the familiar face or the novel one. Finally, frequencies of mouth movements were greater, and latencies of appearance of the first mouth movement were shorter, in front of a static vs. a dynamic face. These results are congruent with the idea of the existence of “a sense” for interaction at birth, and therefore new approaches in newborn studies are discussed.
The Therapeutic Relationship in EMDR Therapy
Authors: Hase Michael , Brisch Karl Heinz
Published: June 2022

The history of EMDR Therapy goes back to 1987, when it was introduced as EMD, a novel treatment for PTSD by Francine Shapiro. Over the course of time EMD developed into the comprehensive therapy approach named EMDR Therapy. The development of the “Adaptive Information Processing (AIP) Model”, the model of pathogenesis and change of EMDR Therapy, was a milestone in this development from technique to psychotherapy approach. Up to date EMDR Therapy offers not only a model of pathogenesis and change, but also a variety of treatment plans and techniques to treat patients of various diagnosis far beyond PTSD. What seems to be missing is a specific description of the therapeutic relationship in EMDR Therapy. The therapeutic relationship should be described as a core element of EMDR Therapy, and seems be related to the structure of EMDR Therapy. As attachment theory offers a view on the development of interpersonal relationships in general, an attachment theory based perspective of the therapeutic relationship seems advisable. A description of the therapeutic relationship in EMDR Therapy is necessary at this point of the development of EMDR Therapy to a psychotherapeutic approach and therefore we try to describe the therapeutic relationship in this article and point out parallels between the therapeutic relationship and the development and core features of an attachment based relationship. We propose to describe EMDR Therapy as a sensitive psychotherapy. Implications for treatment, training and research will be discussed.
Evaluation of Psychometric Properties of Hardiness Scales: A Systematic Review
Authors: Sharif Nia Hamid , Froelicher Erika Sivarajan , Hosseini Lida , Ashghali Farahani Mansoureh
Published: June 2022

Background

Hardiness is one of the personality traits that can help individuals in stressful situations. Since human beings are constantly under stressful situations and the stresses inflicted on people in each situation are different, various scales have been developed for assessing this feature among different people in different situations. Hence, it becomes necessary for researchers and health workers to assess this concept with valid and reliable scales. This systematic review aims to rigorously assess the methodological quality and psychometric properties of hardiness scales.

Method

In the first step, the databases including Scopus, PubMed, Web of science, and Persian databases were searched using suitable keywords without limitation time. We select eligible suitable studies after screening titles and abstracts. The quality of studies was evaluated using the Consensus-based Standards for the selection of health Measurement Instruments (COSMIN) checklist and the Terwee quality criteria.

Result

Of the 747 articles identified, 33 articles were entered in this study. Based on the COSMIN checklist, the most reported properties were as following structural validity (84%), hypothesis testing (56%), content validity (42%), and internal consistency (39%). Furthermore, 12 studies reported cross-cultural validity, three studies criterion validity, and one study reported measurement error.

Conclusion

The “family caregivers’ hardiness scale,” “Japanese Athletic Hardiness Scale,” “Occupational Hardiness Questionnaire,” and “Children’s Hardiness Scale” are the best tools for assessing hardiness in family caregivers, athletes, employees, and children respectively. In addition, the “Dispositional Resilience Scale” (DRS-15) and The Personal Views Survey (PVS III-R) are the most frequently used scales with suitable features for measuring hardiness in the general population.
The Effect of Ingroup vs. Outgroup Members' Behavior on Charity Preference: A Drift-Diffusion Model Approach
Authors: Reich Lars M. , Mahr Luisa A. M. , Vacondio Martina , Khalid Afreen S.
Published: June 2022

Providing potential donors with information about the behavior of others (i.e., social information) is an increasingly used strategy to nudge prosocial decision-making. In the present study, we investigated the effect of ingroup vs. outgroup information on participants' charity preferences by applying a Drift Diffusion Model (DDM) approach. In a joint evaluation scenario, we manipulated different levels of ingroup/outgroup preference ratios for two charities within subjects. Every subject was presented with three stimulus types (i.e., high, medium, and low ingroup ratio) randomized in 294 trials divided into six blocks. We expected that for stimuli with a high ingroup/outgroup ratio, participants should more often and faster decide for the ingroup's most favored charity. We expected that the speed of evidence accumulation will be higher the larger the ingroup/outgroup ratio. Additionally, we investigated whether variations in model parameters can explain individual differences in participants' behaviors. Our results showed that people generally followed ingroup members' preferences when deciding for a charity. However, on finding an unexpected pattern in our results, we conductedpost-hocanalyses which revealed two different behavioral strategies used by participants. Based on participants' decisions, we classified them into “equality driven” individuals who preferred stimuli with the least difference between ingroup and outgroup percentages or “ingroup driven” individuals who favored stimuli with the highest ingroup/outgroup ratio. Results are discussed in line with relevant literature, and implications for practitioners are given.