bluuebunny/crossref_metadata_2025 huggingface dataset:
The effect of phytosulfokine alpha on haploid embryogenesis and gene expression of Brassica napus microspore cultures
Authors: Mestinšek Mubi Špela , Kunej Urban , Vogrinčič Valentin , Jakše Jernej , Murovec Jana
Published: February 2024

Microspore embryogenesis (ME) is the most powerful tool for creating homozygous lines in plant breeding and molecular biology research. It is still based mainly on the reprogramming of microspores by temperature, osmotic and/or nutrient stress. New compounds are being sought that could increase the efficiency of microspore embryogenesis or even induce the formation of haploid embryos from recalcitrant genotypes. Among these, the mitogenic factor phytosulfokine alpha (PSK-α) is promising due to its broad spectrum of activity in vivo and in vitro. The aim of our study was to investigate the effect of PSK-α on haploid embryogenesis from microspores of oilseed rape (Brassica napus L., DH4079), one of the most important oil crops and a model plant for studying the molecular mechanisms controlling embryo formation. We tested different concentrations (0, 0.01, 0.1 and 1 µM) of the peptide and evaluated its effect on microspore viability and embryo regeneration after four weeks of culture. Our results showed a positive correlation between addition of PSK-α and cultured microspore viability and a positive effect also on the number of developed embryos. The analysis of transcriptomes across three time points (day 0, 2 and 4) with or without PSK-α supplementation (15 RNA libraries in total) unveiled differentially expressed genes pivotal in cell division, microspore embryogenesis, and subsequent regeneration. PCA grouped transcriptomes by RNA sampling time, with the first two principal components explaining 56.8% variability. On day 2 with PSK, 45 genes (15 up- and 30 down-regulated) were differentially expressed when PSK-α was added and their number increased to 304 by day 4 (30 up- and 274 down-regulated). PSK, PSKR, and PSI gene expression analysis revealed dynamic patterns, with PSK2 displaying the highest increase and overall expression during microspore culture at days 2 and 4. Despite some variations, only PSK1 showed significant differential expression upon PSK-α addition. Of 16 ME-related molecular markers, 3 and 15 exhibited significant differential expression in PSK-supplemented cultures at days 2 and 4, respectively. Embryo-specific markers predominantly expressed after 4 days of culture, with higher expression in medium without PSK, while on day 0, numerous sporophyte-specific markers were highly expressed.
Changes in the degree of lateral root trait plasticity and trade-offs of maize under long-term no tillage
Authors: Yin Liming , Lv Qiushuang , Wang Peng , Xie Hongtu
Published: February 2024

Introduction

While no tillage (NT) can significantly influence soil structure stratification compared to conventional tillage (CT), a comprehensive understanding of the degree of root trait plasticity and trade-offs of lateral roots of crops at various growth stages along a deep soil profile in response to NT remains elusive. This knowledge gap is important for understanding soil resource acquisition strategies and yield of crops.

Methods

We systematically investigated the traits of lateral roots at jointing and flowering stages in a long-term (12 years) experiment in Northeast China where maize (Zea mays) has been continuously planted under CT and NT with or without maize residue mulch on soil surface. We also measured soil penetration resistance and bulk density.

Results

Soil penetration resistance was reduced at the jointing stage, and was increased at the flowering stage under NT especially at a depth of 10 - 40 cm. Root length density decreased under NT across the two growth stages by on average 22%. In contrast, specific root length and diameter showed greater plasticity, ranging from -14% to 20% and from -11% to 8%, respectively, relative to those under CT.

Discussion

These responses could be attributed to changes in root length proportions with different diameters associated with differences in soil penetration resistance between tillage practices. The negative relationships between root traits were stronger under CT than NT, and became weaker from the jointing stage to the flowering stage. To the best of our knowledge, for the first time, our study provides empirical evidence for pivotal root trait plasticity and trade-offs across growth stages as key indicators of changes in soil structure and resources in response to NT. These insights contribute to a better understanding of soil resource acquisition strategies of crops under NT.
Effects of students’ perception of teachers’ ethnic-racial socialization on students’ ethnic identity and mental health in rural China’s schools
Authors: Lai Angel Hor Yan , Lam Jason K. H. , Yao Hong , Tsui Elaine , Leung Cynthia
Published: February 2024

Introduction

Using students in the Liangshan Yi autonomous prefectures of southwestern China (n = 585; 13–15 years old), we examined (i) the effects of students’ perception of their teachers’ ethnic-racial socialization on their ethnic identity and mental health outcomes of depressive and stress symptoms; (ii) the effects of students’ ethnic identity on their depressive and stress symptoms; (iii) the differential associations among these factors in Yi ethnic minority versus Han cultural majority students.

Methods

We conducted a cross-sectional survey and used multistage sampling to collect the information. Chinese-validated standardized measures were used: the Patient Health Questionaires-9, Children’s Revised Impact of Event Scale-8, Multigroup Ethnic Identity Measure, Cultural Socialization Scale, and Teachers’ Attitude on Adoption of Cultural Diversity Scale. Multigroup confirmatory factor analysis and multigroup structural equation modeling were employed.

Results

Comparing the findings in Yi and Han students, their perception of teachers’ ethnic-racial socialization had dissimilar effects on their ethnic identity and mental health outcomes. Three key findings comparing the differences between Yi and Han students were as follows: (i) students’ perception of their teachers’ multicultural socialization practices positively affected the ethnic identity of both Yi and Han young people; however, their perception of their teachers’ socializing them to their own cultures did not exert any effect; (ii) students’ perception of teachers’ multicultural socialization practices had different mental health effects on Yi versus Han students; and (iii) ethnic identity affected the mental health of Yi ethnic minority students only.

Conclusion

The findings underscore the importance of teachers’ multicultural socialization in the ethnic identity development of both Yi ethnic minority and Han majority students. Ethnic identity serves as a linking variable bridging perceived teachers’ multicultural socialization practices and mental health in Yi ethnic minority students but not among the Han cultural majority youths. Research, practice, and policy implications relevant to the global context are also discussed.
Ambient and focal attention during complex problem-solving: preliminary evidence from real-world eye movement data
Authors: Guo Yuxuan , Pannasch Sebastian , Helmert Jens R. , Kaszowska Aleksandra
Published: February 2024

Time course analysis of eye movements during free exploration of real-world scenes often reveals an increase in fixation durations together with a decrease in saccade amplitudes, which has been explained within the two visual systems approach, i.e., a transition from ambient to focal. Short fixations and long saccades during early viewing periods are classified as ambient mode of vision, which is concerned with spatial orientation and is related to simple visual properties such as motion, contrast, and location. Longer fixations and shorter saccades during later viewing periods are classified as focal mode of vision, which is concentrated in the foveal projection and is capable of object identification and its semantic categorization. While these findings are mainly obtained in the context of image exploration, the present study endeavors to investigate whether the same pattern of interplay between ambient and focal visual attention is deployed when people work on complex real-world tasks—and if so, when? Based on a re-analysis of existing data that integrates concurrent think aloud and eye tracking protocols, the present study correlated participants’ internal thinking models to the parameters of their eye movements when they planned solutions to an open-ended design problem in a real-world setting. We hypothesize that switching between ambient and focal attentional processing is useful when solvers encounter difficulty compelling them to shift their conceptual direction to adjust the solution path. Individuals may prefer different attentional strategies for information-seeking behavior, such as ambient-to-focal or focal-to-ambient. The observed increase in fixation durations and decrease in saccade amplitudes during the periods around shifts in conceptual direction lends support to the postulation of the ambient-to-focal processing; however, focal-to-ambient processing is not evident. Furthermore, our data demonstrate that the beginning of a shift in conceptual direction is observable in eye movement behavior with a significant prolongation of fixation. Our findings add to the conclusions drawn from laboratory settings by providing preliminary evidence for ambient and focal processing characteristics in real-world problem-solving.
Longitudinal assessment of psychological distress and its determinants in a sample of firefighters based in Montreal, Canada
Authors: Rapisarda Filippo , Guay Stéphane , Ouellet-Morin Isabelle , Bond Suzie , Geoffrion Steve
Published: February 2024

Introduction

Firefighters face elevated risks of common mental health issues, with distress rates estimated at around 30%, surpassing those of many other occupational groups. While exposure to potentially traumatic events (PTEs) is a well-recognized risk factor, existing research acknowledges the need for a broader perspective encompassing multidimensional factors within the realm of occupational stress. Furthermore, this body of evidence heavily relies on cross-sectional studies. This study adopts an intensive longitudinal approach to assess psychological distress and its determinants among firefighters.

Methods

Participants were recruited from 67 fire stations in Montreal, Canada, meeting specific criteria: full-time employment, smartphone ownership, and recent exposure to at least one PTE, or first responder status. Subjects underwent a telephone interview and were directed to use an app to report depressive, post-traumatic, and generalized anxiety symptoms every 2 weeks, along with work-related stressors, social support, and coping styles. Analyses involved 274 participants, distinguishing between those exceeding clinical thresholds in at least one distress measure (the “distressed” subgroup) and those deemed “resilient.” The duration and onset of distress were computed for the distressed group, and linear mixed models were employed to evaluate determinants for each psychological distress variable.

Results

Clinical psychological distress was observed in 20.7% of participants, marked by depressive, post-traumatic, and anxiety symptoms, often within the first 4-week reference period. Contextual factors (operational climate, social support, solitude) and individual factors (coping style, solitude and lifetime traumatic events in private life) exhibited more significant impacts on psychological distress than professional pressures within the firefighters’ work environment.

Discussion

This study reports lower rates of psychological distress than previous research, possibly attributable to sample differences. It highlights that reported symptoms often represent a combined and transient layer of distress rather than diagnosable mental disorders. Additionally, determinants analysis underscores the importance of interpersonal relationships and coping mechanisms for mental health prevention interventions within this worker group. The findings carry implications for the development of prevention and support programs for firefighters and similar emergency workers.
Fostering organisational commitment: a resilience framework for private-sector organisations in South Africa
Authors: Mujajati Ester , Ferreira Nadia , du Plessis Melissa
Published: February 2024

Introduction

Organisations worldwide encounter three significant and challenging issues related to talent management: intense competition for skilled employees, elevated rates of employee attrition, and the ongoing struggle to attract top-tier talent. This research focused on investigating the interconnected dynamics among factors associated with employee retention, including organisational commitment, job embeddedness, and hardiness, in conjunction with resilience-related behaviours such as resilience and career adaptability.

Methods

A cross-sectional survey design was used to gather quantitative data from a convenience sample of employees within the private sector in South Africa (N = 293). The self-assessments of the participants were assessed using a range of well-established and validated instruments. Correlation and regression analyses, followed by structural equation modelling, were utilised to construct a resilience framework designed specifically for private sector organisations in South Africa.

Results

The results reveal significant associations between organisational commitment, job embeddedness, and hardiness (as retention-related attributes) and resilience and career adaptability (as resilience-related behavioural capacities). These relationships served as the basis for the development of a resilience framework for employees in South African private organisations.

Discussion

In South African private-sector organisations, talent retention is crucial due to a talent shortage. The study found that employees have a strong emotional attachment to their organisations, are highly aligned with their jobs and communities, and display resilience. Organisational commitment, job embeddedness, and hardiness are key factors in reducing turnover, forming an effective retention strategy. This research contributes to the development of a resilience framework for South African private sector organisations.
The causal effect of air pollution on the risk of essential hypertension: a Mendelian randomization study
Authors: Xia Zhiwei , Liu Yinjiang , Liu Chao , Dai Ziyu , Liang Xisong , Zhang Nan , Wu Wantao , Wen Jie , Zhang Hao
Published: February 2024

Background

Air pollution poses a major threat to human health by causing various illnesses, such as cardiovascular diseases. While plenty of research indicates a correlation between air pollution and hypertension, a definitive answer has yet to be found.

Methods

Our analyses were performed using the Genome-wide association study (GWAS) of exposure to air pollutants from UKB (PM2.5, PM10, NO2, and NOX; n = 423,796 to 456,380), essential hypertension from FinnGen (42,857 cases and 162,837 controls) and from UKB (54,358 cases and 408,652 controls) as a validated cohort. Univariable and multivariable Mendelian randomization (MR) were conducted to investigate the causal relationship between air pollutants and essential hypertension. Body mass index (BMI), alcohol intake frequency, and the number of cigarettes previously smoked daily were included in multivariable MRs (MVMRs) as potential mediators/confounders.

Results

Our findings suggested that higher levels of both PM2.5 (OR [95%CI] per 1 SD increase in predicted exposure = 1.24 [1.02–1.53], p = 3.46E-02 from Finn; OR [95%CI] = 1.04 [1.02–1.06], p = 7.58E-05 from UKB) and PM10 (OR [95%CI] = 1.24 [1.02–1.53], p = 3.46E-02 from Finn; OR [95%CI] = 1.04 [1.02–1.06], p = 7.58E-05 from UKB) were linked to an increased risk for essential hypertension. Even though we used MVMR to adjust for the impacts of smoking and drinking on the relationship between PM2.5 exposure and essential hypertension risks, our findings suggested that although there was a direct positive connection between them, it is not present after adjusting BMI (OR [95%CI] = 1.05 [0.87–1.27], p = 6.17E-01). Based on the study, higher exposure to PM2.5 and PM10 increases the chances of developing essential hypertension, and this influence could occur through mediation by BMI.

Conclusion

Exposure to both PM2.5 and PM10 is thought to have a causal relationship with essential hypertension. Those impacted by substantial levels of air pollution require more significant consideration for their cardiovascular health.
Imaging of intracranial arterial disease: a comparison between MRI and unenhanced CT
Authors: Lucci Carlo , Rissanen Ina , Takx Richard A. P. , van der Kolk Anja G. , Harteveld Anita A. , Dankbaar Jan W. , Geerlings Mirjam I. , de Jong Pim A. , Hendrikse Jeroen
Published: February 2024

Background and purpose

Arterial calcifications on unenhanced CT scans and vessel wall lesions on MRI are often used interchangeably to portray intracranial arterial disease. However, the extent of pathology depicted with each technique is unclear. We investigated the presence and distribution of these two imaging findings in patients with a history of cerebrovascular disease.

Materials and methods

We analyzed CT and MRI data from 78 patients admitted for stroke or TIA at our institution. Vessel wall lesions were assessed on 7 T MRI sequences, while arterial calcifications were assessed on CT scans. The number of vessel wall lesions, severity of intracranial internal carotid artery (iICA) calcifications, and overall presence and distribution of the two imaging findings were visually assessed in the intracranial arteries.

Results

At least one vessel wall lesion or arterial calcification was assessed in 69 (88%) patients. Only the iICA and vertebral arteries (VA) showed a substantial number of both calcifications and vessel wall lesions. The other vessels showed almost exclusively vessel wall lesions. The number of vessel wall lesions was associated with the severity of iICA calcification (p = 0.013).

Conclusions

The number of vessel wall lesions increases with the severity of iICA calcifications. Nonetheless, the distribution of vessel wall lesions on MRI and arterial calcifications on CT shows remarkable differences. These findings support the need for a combined approach to examine intracranial arterial disease.
The structural modelling of significant organisational and individual factors for promoting sustainable campus in Saudi Arabia
Authors: Alsharif Mohammad Ambarak
Published: February 2024

Introduction

In recent years, campus sustainability has gained global prominence, with a focus on sustainability initiatives and environmental activities within university campuses. This study aims to explore the factors contributing to the expansion of sustainability initiatives in Saudi Arabian university campuses.

Methods

The research utilizes the Interpretive Structural Model (ISM) as a framework for public universities. A literature review incorporating rational choice theory (RCT) and organizational change theory (OCT) is conducted to identify variables relevant to campus sustainability. The hierarchical interrelationships among these variables are examined, and the Matrice d’Impacts Croises-Multiplication Appliqué and Classment Analysis (MICMAC) categorize the study variables. Associations between the variables are validated through expert consultations from selected case study institutions.

Results

The findings highlight the significance of organizational structure and decision-making processes in determining the prevalence of other sustainability-related variables. Organizational structure and decision-making processes are recognized as key factors exerting significant influence over other variables. Additionally, leadership is categorized as a linkage variable, representing a strong relationship within the hierarchical model.

Discussion

The study acknowledges the challenges in implementing sustainability initiatives, including conflicts in strategic planning, absence of campus sustainability policies and regulations, financial barriers, and lack of support from leadership. The study offers valuable insights and recommendations for stakeholders involved in sustainability initiatives within higher education institutions, particularly university leaders and decision makers in facility and project management departments (F&PM). By addressing the organizational and decision-based factors, effective integration of sustainability practices into university planning and activities can be achieved. This research contributes to filling the knowledge gap regarding the factors influencing the implementation of sustainability in Saudi Arabian higher education institutions (HEIs). The study emphasizes the importance of addressing organizational structure, decision-making processes, and leadership in order to successfully implement sustainability practices on university campuses.
Computational inference of chemokine-mediated roles for the vagus nerve in modulating intra- and inter-tissue inflammation
Authors: Shah Ashti M. , Zamora Ruben , Barclay Derek , Yin Jinling , El-Dehaibi Fayten , Addorisio Meghan , Tsaava Tea , Tynan Aisling , Tracey Kevin , Chavan Sangeeta S. , Vodovotz Yoram
Published: February 2024

Introduction: The vagus nerve innervates multiple organs, but its role in regulating cross-tissue spread of inflammation is as yet unclear. We hypothesized that the vagus nerve may regulate cross-tissue inflammation via modulation of the putatively neurally regulated chemokine IP-10/CXCL10.

Methods: Rate-of-change analysis, dynamic network analysis, and dynamic hypergraphs were used to model intra- and inter-tissue trends, respectively, in inflammatory mediators from mice that underwent either vagotomy or sham surgery.

Results: This analysis suggested that vagotomy primarily disrupts the cross-tissue attenuation of inflammatory networks involving IP-10 as well as the chemokines MIG/CXCL9 and CCL2/MCP-1 along with the cytokines IFN-γ and IL-6. Computational analysis also suggested that the vagus-dependent rate of expression of IP-10 and MIG/CXCL9 in the spleen impacts the trajectory of chemokine expression in other tissues. Perturbation of this complex system with bacterial lipopolysaccharide (LPS) revealed a vagally regulated role for MIG in the heart. Further, LPS-stimulated expression of IP-10 was inferred to be vagus-independent across all tissues examined while reducing connectivity to IL-6 and MCP-1, a hypothesis supported by Boolean network modeling.

Discussion: Together, these studies define novel spatiotemporal dimensions of vagus-regulated acute inflammation.
Optimizing Multidimensional Pooling for Variational Quantum Algorithms
Authors: Jeng Mingyoung , Nobel Alvir , Jha Vinayak , Levy David , Kneidel Dylan , Chaudhary Manu , Islam Ishraq , Baumgartner Evan , Vanderhoof Eade , Facer Audrey , Singh Manish , Arshad Abina , El-Araby Esam
Published: February 2024

Convolutional neural networks (CNNs) have proven to be a very efficient class of machine learning (ML) architectures for handling multidimensional data by maintaining data locality, especially in the field of computer vision. Data pooling, a major component of CNNs, plays a crucial role in extracting important features of the input data and downsampling its dimensionality. Multidimensional pooling, however, is not efficiently implemented in existing ML algorithms. In particular, quantum machine learning (QML) algorithms have a tendency to ignore data locality for higher dimensions by representing/flattening multidimensional data as simple one-dimensional data. In this work, we propose using the quantum Haar transform (QHT) and quantum partial measurement for performing generalized pooling operations on multidimensional data. We present the corresponding decoherence-optimized quantum circuits for the proposed techniques along with their theoretical circuit depth analysis. Our experimental work was conducted using multidimensional data, ranging from 1-D audio data to 2-D image data to 3-D hyperspectral data, to demonstrate the scalability of the proposed methods. In our experiments, we utilized both noisy and noise-free quantum simulations on a state-of-the-art quantum simulator from IBM Quantum. We also show the efficiency of our proposed techniques for multidimensional data by reporting the fidelity of results.
Simulation Analysis and Multiobjective Optimization of Pulverization Process of Seed-Used Watermelon Peel Pulverizer Based on EDEM
Authors: Mou Xiaobin , Wan Fangxin , Wu Jinfeng , Luo Qi , Xin Shanglong , Ma Guojun , Zhou Xiaoliang , Huang Xiaopeng , Peng Lizeng
Published: February 2024

To enhance the utilization of seed-used watermelon peel and mitigate environmental pollution, a hammer-blade seed-used watermelon peel crusher was designed and manufactured, and its structure and working parameters were optimized. Initially, the seed-used watermelon peel crusher and seed-used watermelon peel model were constructed, and the model’s parameters were calibrated. Subsequently, the discrete element method (EDEM2022) was employed to investigate the effects of spindle speed (MSS), the number of hammers (NCB), and feeding volume (FQ) on the pulverizing process. Multivariate nonlinear regression prediction models were developed for the percentage of pulverized particle size less than 8 mm (Psv), pulverizing efficiency (Ge), and power density (Ppd), followed by the analysis of influencing factors and prediction models using ANOVA. The multiobjective optimization of the prediction model utilizing the improved hybrid metacellular genetic algorithm CellDE resulted in solutions of 90.02%, 89.57%, and 8.35 × 10−3 t/(h-kw) for Psv-opt, Ge-opt, and Ppd-opt, respectively. The corresponding optimal interaction values of MSS, NCB, and FQ were determined to be 1500 r/min, 108, and 150 kg/min. Finally, a prototype test was conducted by combining the optimal factor interaction values, yielding statistically calculated values of 96.63%, 92.37%, and 7.76 × 10−3 t/(h-kw) for Psv-pr, Ge-pr, and Ppd-pr, respectively. The results indicate that the optimized values of Psv-opt, Ge-opt, and Ppd-opt models have an error of less than 8% compared to the statistically calculated values of the prototype test and outperform the values of Psv-ori, Ge-ori, and Ppd-ori obtained under the original parameters.
An Explanatory Model of Red Lentil Seed Coat Colour to Manage Degradation in Quality during Storage
Authors: Bhattarai Bhawana , Nuttall James G. , Walker Cassandra K. , Wallace Ashley J. , Fitzgerald Glenn J. , O’Leary Garry J.
Published: February 2024

This study presents an explanatory biophysical model developed and validated to simulate seed coat colour traits including CIE L*, a*, and b* changes over time for stored lentil cultivars PBA Hallmark, PBA Hurricane, PBA Bolt, and PBA Jumbo2 under diverse storage conditions. The model showed robust performance for all cultivars, with R2 values ≥ 0.89 and RMSE values ≤ 0.0019 for all seed coat colour traits. Laboratory validation at 35 °C demonstrated a high agreement (Lin’s Concordance Correlation Coefficient, CCC ≥ 0.82) between simulated and observed values of all colour traits for PBA Jumbo2 and strong agreement (CCC ≥ 0.81) for PBA Hallmark in brightness (CIE L*) and redness (CIE a*), but not in yellowness (CIE b*). At 15 °C, both cultivars exhibited moderate to weak agreement between simulated and observed values of all colour traits (CCC ≤ 0.47), as very little change was recorded in the observed values over the 360 days of storage. Bulk storage system validation for PBA Hallmark showed moderate performance (CCC ≥ 0.46) between simulated and observed values of all colour traits. Modelling to simulate changes in seed coat colour traits of lentils over time will equip growers and traders to make informed managerial decisions when storing lentils for long periods.
Combined Passive Heating Systems in Mediterranean, Low-Cost, Greenhouse Cucumber Crops
Authors: Bonachela Santiago , Granados María Rosa , Hernández Joaquín , López Juan Carlos , Magán Juan José
Published: February 2024

Greenhouse microclimate and crop response of winter cucumber cycles grown in unheated Mediterranean greenhouses with representative combinations of passive heating systems (fixed, plastic screen with and without black mulch; movable thermal screen with black mulch; and double-layer plastic covering with black mulch) were evaluated in Almería, SE Spain. In the first experiment, the black mulch in combination with a movable or fixed screen increased the marketable cucumber yield by 14%, which appears to be mostly attributable to higher substrate temperatures induced by the black mulch in the cold period. Moreover, the black mulch in combination with a fixed screen frequently led to screen water condensation. The use of a movable screen, rather than a fixed one, in combination with a black mulch increased the first-class cucumber yield in the second experiment and reduced the non-marketable one in the first experiment. This might be mainly attributable to the higher incoming shortwave radiation in the cold period. Moreover, the movable screen reduced the risk of water condensation on the screen and the crop. The cucumber in the greenhouse with the double-layer covering and black mulch, compared to that with movable screen and black mulch, received lower daily incoming shortwave radiation, particularly, during the second half of the cycle (mainly due to the formation of water condensation droplets on the lower surface of the external plastic film), which reduced crop yield. Further field research is needed to better quantify the most limiting factor for growth (substrate/soil or air temperature, radiation, or water condensation) in Mediterranean greenhouse crops.
Integrated Management of Clubroot in Zhejiang Province, China
Authors: Zhang Yi , Yu Hongrui , Zhao Tong , Hussain Iqbal , Ma Xinyan , Wang Yuqi , Liu Kaiwen , Sun Nairan , Yu Xiaolin
Published: February 2024

Clubroot, caused by Plasmodiophora brassicae, is a destructive soil-borne disease significantly harming global Brassica crop production. This study employed the Williams and European Clubroot Differential (ECD) and Williams systems to identify the pathotypes of P. brassicae collected from Hangzhou City, Yuhang District and Quzhou City, Kaihua County in Zhejiang Province. Greenhouse and field trials were conducted to evaluate the effects of plastic film covering and four chemical agents on the growth parameters and clubroot severity of the Chinese cabbage cultivar ‘Granaat’. Potential treatment mechanisms on clubroot were explored through a qPCR analysis of the resting spore density and pH measurement of the soil. Furthermore, treatment with 1-napthaleneacetic acid (NAA), a synthetic auxin, was also evaluated for its potential role in suppressing clubroot. The results indicate that the pathotypes of P. brassicae in the two districts were P1, ECD20/31/12, and P3, ECD20/15/4. While an individual application of plastic film covering could not effectively control clubroot, calcium cyanamid, dazomet and ammonium bicarbonate demonstrated significant efficacy in its management. These three agents significantly reduced the resting spore density in the soil, with calcium cyanamid and ammonium bicarbonate also increasing soil alkalinity. Additionally, ammonium bicarbonate promotes lateral root development in ‘Granaat,’ helping infected plants access adequate water and nutrients. However, NAA exhibited no efficacy in clubroot control. Therefore, sustained lateral root development is crucial for effectively resisting P. brassicae invasion. Considering application costs and environmental friendliness, we propose the field application of ammonium bicarbonate as the optimal method for clubroot disease management in Zhejiang Province.
Effect of Dietary Supplementation of Chestnut and Quebracho Tannin Supplementation on Neonatal Diarrhoea in Preweaning Calves
Authors: Dell’Anno Matteo , Frazzini Sara , Ferri Irene , Tuberti Susanna , Bonaldo Elisa , Botti Benedetta , Grossi Silvia , Sgoifo Rossi Carlo Angelo , Rossi Luciana
Published: February 2024

Neonatal calf diarrhoea (NCD) poses a significant health challenge in cattle herds, resulting in considerable economic losses and antimicrobial use. In response to the escalating threat of antimicrobial resistance, viable alternatives are imperative, aligning with European policies. This study evaluated the in-milk supplementation of the chestnut and quebracho tannin extract in preweaning calves on performance, diarrhoea occurrence, Cryptosporidium spp. shedding, protein digestibility, and intestinal health. Twenty newborn calves were divided, after colostrum administration, into two experimental groups for 30 days as follows: the control (CTRL) was fed with whole milk and solid feed, and tannins (TAN) were fed whole milk supplemented with 6/g day of tannin extract and solid feed. Faecal samples were collected on days 0, 3, 7, 14, and 30 for the evaluation of Cryptosporidium oocyst shedding and protein digestibility. Faecal consistency was evaluated during the sampling using the faecal score scale (0–3 scale, considering diarrhoea > 1). The results showed a significant reduction in diarrhoea frequency in the TAN compared to the CTRL group (p < 0.05) over 30 days of the trial. The prevalence of Cryptosporidium spp. was generally low (12%), considering all analysed samples. Protein digestibility revealed comparable values for the TAN and CTRL groups, suggesting that tannins did not negatively affect milk protein availability. In conclusion, the in-milk supplementation of 6/g day of the chestnut and quebracho tannin extract could be considered a valuable functional feed additive to decrease NCD occurrence, thus supporting animal health and decreasing antibiotic use in livestock.
Optimization, Metabolomic Analysis, Antioxidant Potential and
Depigmenting Activity of Polyphenolic Compounds from
Unmature Ajwa Date Seeds (Phoenix dactylifera L.) Using Ultrasonic-Assisted Extraction
Authors: Alshammari Fanar , Alam Md Badrul , Naznin Marufa , Kim Sunghwan , Lee Sang-Han
Published: February 2024

This study sought to optimize the ultrasonic-assisted extraction of polyphenolic compounds from unmature Ajwa date seeds (UMS), conduct untargeted metabolite identification and assess antioxidant and depigmenting activities. Response surface methodology (RSM) utilizing the Box–Behnken design (BBD) and artificial neural network (ANN) modeling was applied to optimize extraction conditions, including the ethanol concentration, extraction temperature and time. The determined optimal conditions comprised the ethanol concentration (62.00%), extraction time (29.00 min), and extraction temperature (50 °C). Under these conditions, UMS exhibited total phenolic content (TPC) and total flavonoid content (TFC) values of 77.52 ± 1.55 mgGAE/g and 58.85 ± 1.12 mgCE/g, respectively, with low relative standard deviation (RSD%) and relative standard error (RSE%). High-resolution mass spectrometry analysis unveiled the presence of 104 secondary metabolites in UMS, encompassing phenols, flavonoids, sesquiterpenoids, lignans and fatty acids. Furthermore, UMS demonstrated robust antioxidant activities in various cell-free antioxidant assays, implicating engagement in both hydrogen atom transfer and single electron transfer mechanisms. Additionally, UMS effectively mitigated tert-butyl hydroperoxide (t-BHP)-induced cellular reactive oxygen species (ROS) generation in a concentration-dependent manner. Crucially, UMS showcased the ability to activate mitogen-activated protein kinases (MAPKs) and suppress key proteins including tyrosinase (Tyr), tyrosinase-related protein-1 and -2 (Trp-1 and -2) and microphthalmia-associated transcription factor (MITF), which associated melanin production in MNT-1 cell. In summary, this study not only optimized the extraction process for polyphenolic compounds from UMS but also elucidated its diverse secondary metabolite profile. The observed antioxidant and depigmenting activities underscore the promising applications of UMS in skincare formulations and pharmaceutical developments.
Research on Energy Absorption Characteristics of Polypropylene Foam Concrete Buffer Layer in High Ground Stress Soft Rock Tunnel
Authors: Jili Ciqie , Lu Junfu , Lu Hanyu , Miao Zhihao
Published: February 2024

Creep stress is a detrimental stress generated by the surrounding rock during the operation of the secondary lining of the supporting structure used for high-stress soft rock tunnels in railways, highways and other projects. Therefore, the present study aims to investigate and provide solutions for damages such as cracking and deformation caused by creep stress. For this purpose, research methods used in the literature and experimental studies, as well as the theoretical, data and numerical simulation analyses, were used. Accordingly, the mix proportion design, specimen production and the tests of physical and mechanical properties of EPP foam concrete were carried out. Moreover, the EPP(polypropylene foam) concrete was used as the buffer layer of the supporting structure, the practical application of which was verified through numerical analyses. The findings of the study revealed the effective compression performance of the mix proportion design method. Furthermore, the EPP foam concrete was found to be able to absorb the energy generated by the creep of the surrounding rock, consequently reducing the surrounding rock pressure acting on the secondary lining structure, which, in turn, ensures the safety of the operation. The findings of the study can be used as a reference for designing similar projects.
Phase Angle and Bioelectrical Impedance Vector Analysis (BIVA) in Amyotrophic Lateral Sclerosis (ALS) Patients
Authors: Cunha Thais Alves , Lopes Márcia Marília Gomes Dantas , de Araújo Brito Acsa Nara , Vermeulen-Serpa Karina Marques , de Lima Vale Sancha Helena , Brandão-Neto José , Leite-Lais Lucia
Published: February 2024

Phase angle (PhA) and bioelectrical impedance vector analysis (BIVA) have emerged as valuable tools for assessing nutritional status and prognosis in various patient populations, but there is a lack of studies in rare neurodegenerative diseases. The purpose of this cross-sectional study was to investigate these bioelectrical parameters in patients with amyotrophic lateral sclerosis (ALS), compared with healthy peers. The tetrapolar impedance method was applied and bioimpedance analysis (BIA) was performed. Bioelectrical parameters were obtained (Resistance—R; Reactance—Xc) or calculated (PhA; BIVA). For BIVA, bivariate vectors and confidence ellipses were graphically represented. In addition, R and Xc were used to determine body composition (BC) (Fat Mass—FM; Fat-Free Mass—FFM; and Total Body Water—TBW). In this study, 40 participants were divided into two groups: case group (n = 20, ALS patients) and control group (n = 20, healthy subjects. Our main results showed that ALS patients presented low levels of BMI, FFM, R, and Xc adjusted by height (R/H, Xc/H), hydration, and cellularity, compared to the healthy subjects. Our findings highlight BC and bioelectrical parameters, including PhA and BIVA, as valuable indicators of nutrition status, which should be implemented in the nutrition care process of ALS patients during the disease course.
Revealing the Impact of Ga and Y Doping on Thermal and Electrical Behavior of LaMnO3 Ceramic Materials
Authors: Vlazan Paulina , Marin Catalin Nicolae , Malaescu Iosif , Vlase Gabriela , Vlase Titus , Poienar Maria , Sfirloaga Paula
Published: February 2024

The synthesis, thermal behavior and electrical properties of a series of undoped and 1% Ga- or Y-doped lanthanum manganite compounds, obtained via the sol–gel technique, are reported. Scanning electron microscopy (SEM/EDX), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR) analyses were performed on all synthesized samples. The XRD results confirmed a good crystallinity for all studied samples, and a change in the crystal structure of Ga- or Y-doped lanthanum manganite (Pm-3m space group) was observed compared to the pristine sample (R-3c space group). Thermal analysis highlighted a different behavior of the doped samples compared to the undoped sample, observed by the different mass losses in the analyzed temperature range. For these materials, it is shown for the first time that the static electrical conductivity, σDC, of Ga- or Y-doped LaMnO3 compounds increases compared to the σDC of the pristine sample, and the thermal activation energy of the process of electrical conduction, EA,cond, increases linearly with the temperature for all three studied samples.
From Posts to Knowledge: Annotating a Pandemic-Era Reddit Dataset to Navigate Mental Health Narratives
Authors: Rani Saima , Ahmed Khandakar , Subramani Sudha
Published: February 2024

Mental illness is increasingly recognized as a substantial public health challenge worldwide. With the advent of social media, these platforms have become pivotal for individuals to express their emotions, thoughts, and experiences, thereby serving as a rich resource for mental health research. This paper is devoted to the creation of a comprehensive dataset and an innovative data annotation methodology to explore the underlying causes of these mental health issues. Our approach included the extraction of over one million Reddit posts from five different subreddits, spanning the pre-pandemic, during-pandemic, and post-pandemic periods. These posts were methodically annotated using a set of specific criteria, aimed at identifying various root causes. This rigorous process produced a richly categorized dataset, invaluable for detailed analysis. The complete unlabelled dataset, along with a subset that has been expertly annotated, is prepared for public release, as outlined in the data availability section. This dataset is a critical resource for training and fine-tuning machine learning models to identify the foundational triggers of individual mental health issues, offering valuable insights for practical interventions and future research in this domain.
Development of Compact and Robust Physical System for Strontium Optical Lattice Clock
Authors: Chen Yingxin , Zhou Chihua , Tan Wei , Guo Feng , Zhao Guodong , Xia Jian , Meng Junwei , Chang Hong
Published: February 2024

Compact and robust optical clocks are significant in scientific research and engineering. Here, we present a physical system for a strontium atomic optical clock with dimensions of 465 mm × 588 mm × 415 mm and a weight of 66.6 kg. To date, this is one of the most compact physical systems ever reported. The application of the magnetic shielding box in this physical system allowed the effect of external magnetic field fluctuation on cold atoms to be negligible. The physical system passed rigorous environmental tests and remained operational. A wavelength meter integrated in this physical system could monitor the wavelengths of the incident laser, and it could automatically calibrate the wavelengths of all lasers using a microcomputer. This compact and robust physical system could be a hardware basis for demonstrating a portable optical clock or even a space optical clock.
Speech Emotion Recognition Using Deep Learning Transfer Models and Explainable Techniques
Authors: Kim Tae-Wan , Kwak Keun-Chang
Published: February 2024

This study aims to establish a greater reliability compared to conventional speech emotion recognition (SER) studies. This is achieved through preprocessing techniques that reduce uncertainty elements, models that combine the structural features of each model, and the application of various explanatory techniques. The ability to interpret can be made more accurate by reducing uncertain learning data, applying data in different environments, and applying techniques that explain the reasoning behind the results. We designed a generalized model using three different datasets, and each speech was converted into a spectrogram image through STFT preprocessing. The spectrogram was divided into the time domain with overlapping to match the input size of the model. Each divided section is expressed as a Gaussian distribution, and the quality of the data is investigated by the correlation coefficient between distributions. As a result, the scale of the data is reduced, and uncertainty is minimized. VGGish and YAMNet are the most representative pretrained deep learning networks frequently used in conjunction with speech processing. In dealing with speech signal processing, it is frequently advantageous to use these pretrained models synergistically rather than exclusively, resulting in the construction of ensemble deep networks. And finally, various explainable models (Grad CAM, LIME, occlusion sensitivity) are used in analyzing classified results. The model exhibits adaptability to voices in various environments, yielding a classification accuracy of 87%, surpassing that of individual models. Additionally, output results are confirmed by an explainable model to extract essential emotional areas, converted into audio files for auditory analysis using Grad CAM in the time domain. Through this study, we enhance the uncertainty of activation areas that are generated by Grad CAM. We achieve this by applying the interpretable ability from previous studies, along with effective preprocessing and fusion models. We can analyze it from a more diverse perspective through other explainable techniques.
On Hybrid Nanogrids Energy Management Systems—An Insight into Embedded Systems
Authors: Bitar Maria , El Tawil Tony , Benbouzid Mohamed , Dinh Van Binh , Benaouicha Mustapha
Published: February 2024

In recent years, the growing demand for efficient and sustainable energy management has led to the development of innovative solutions for embedded systems. One such solution is the integration of hybrid nanogrid energy management systems into various applications. There are currently many energy management systems in different domains, such as buildings, electric vehicles, or even naval transport. However, an embedded nanogrid management system is subject to several constraints that are not sufficiently studied in the literature. Indeed, such a system often has a limited energy reserve and is isolated from any energy supply for a long time. This paper aims to provide a comprehensive overview of the current state of research, advancements, and challenges in the field of hybrid nanogrid energy management systems. Furthermore, it offers a comparative analysis between hybrid nanogrids and microgrids and the implications of their integration in embedded systems. This paper also discusses the key components, operation principles, optimization strategies, real-world implementations, challenges, and future prospects of hybrid nanogrid energy management systems. Moreover, it highlights the significance of such systems in enhancing energy efficiency, reducing carbon footprints, and ensuring reliable power supply.