bluuebunny/crossref_metadata_2025 huggingface dataset:
Digital assessment of cognition in neurodegenerative disease: a data driven approach leveraging artificial intelligence
Authors: Libon David J. , Swenson Rod , Price Catherine C. , Lamar Melissa , Cosentino Stephanie , Bezdicek Ondrej , Kling Mitchel A. , Tobyne Sean , Jannati Ali , Banks Russell , Pascual-Leone Alvaro
Published: July 2024

Introduction

A rapid and reliable neuropsychological protocol is essential for the efficient assessment of neurocognitive constructs related to emergent neurodegenerative diseases. We developed an AI-assisted, digitally administered/scored neuropsychological protocol that can be remotely administered in ~10 min. This protocol assesses the requisite neurocognitive constructs associated with emergent neurodegenerative illnesses.

Methods

The protocol was administered to 77 ambulatory care/memory clinic patients (56.40% women; 88.50% Caucasian). The protocol includes a 6-word version of the Philadelphia (repeatable) Verbal Learning Test [P(r)VLT], three trials of 5 digits backward from the Backwards Digit Span Test (BDST), and the “animal” fluency test. The protocol provides a comprehensive set of traditional “core” measures that are typically obtained through paper-and-pencil tests (i.e., serial list learning, immediate and delayed free recall, recognition hits, percent correct serial order backward digit span, and “animal” fluency output). Additionally, the protocol includes variables that quantify errors and detail the processes used in administering the tests. It also features two separate, norm-referenced summary scores specifically designed to measure executive control and memory.

Results

Using four core measures, we used cluster analysis to classify participants into four groups: cognitively unimpaired (CU; n = 23), amnestic mild cognitive impairment (MCI; n = 17), dysexecutive MCI (n = 23), and dementia (n = 14). Subsequent analyses of error and process variables operationally defined key features of amnesia (i.e., rapid forgetting, extra-list intrusions, profligate responding to recognition foils); key features underlying reduced executive abilities (i.e., BDST items and dysexecutive errors); and the strength of the semantic association between successive responses on the “animal” fluency test. Executive and memory index scores effectively distinguished between all four groups. There was over 90% agreement between how cluster analysis of digitally obtained measures classified patients compared to classification using a traditional comprehensive neuropsychological protocol. The correlations between digitally obtained outcome variables and analogous paper/pencil measures were robust.

Discussion

The digitally administered protocol demonstrated a capacity to identify patterns of impaired performance and classification similar to those observed with standard paper/pencil neuropsychological tests. The inclusion of both core measures and detailed error/process variables suggests that this protocol can detect subtle, nuanced signs of early emergent neurodegenerative illness efficiently and comprehensively.
Variable embodiment of stance-taking and footing in simultaneous interpreting
Authors: Cienki Alan
Published: July 2024

Previous research has argued that consecutive interpreters constitute laminated speakers in the sense that they engage with different kinds of footing at once, representing another’s point of view through their words in another language. These multiple roles also play out in their gesturing, as they sometimes indicate deictically who is the source of the ideas and stances they are expressing (the principal). Simultaneous interpreters, though, often work in an interpreting booth; they are often not seen by the audience, yet many of them gesture, sometimes frequently. How are simultaneous interpreters using gesture in relation to stance-taking and footing? We consider the case of simultaneous interpreters rendering popular science lectures between (both to and from) Russian (their L1) and either English or German (their L2). Though only hearing the audio of the lectures, the interpreters produced many gestures, which were analyzed for their function. Some representational and deictic gestures appeared to clearly involve the interpreter as the principal (writing numbers with one’s finger to help remember them or pointing to two places on the desk to keep track of two different quantities mentioned). Other representational and deictic gestures are ambiguous as to whether they are enacting what the interpreter may have imagined what the lecturer did or whether they arose out of the interpreter’s own thinking for speaking (e.g., tracing the form of a bird being mentioned or pointing to an empty space when the lecturer was referring to a graph). Pragmatic gestures, showing one’s stance toward the topic of the talk, were the most ambiguous as to the footing, reflecting how the interpreter may be engaged in fictive interaction with their imagined audience. Self-adapters, however, more clearly involve the interpreter as the principal, as such actions are known to support cognitive focussing and self-soothing. In sum, we see varying degrees of clarity as to whose stance and principal footing simultaneous interpreters are expressing bodily as laminated speakers. The variable ambiguity can be attributed to the nature of gesture as a semiotic system, the functions of which are more often dependent on co-occurring speech than vice versa.
Promoting health and productivity management in small companies through outreach-based public-private partnership: the Yokohama Linkworker Project
Authors: Kai Yuko , Fujii Yuya , Takashi Naoki , Yoshiba Kaori , Muramatsu-Noguchi Yuko , Noda Takayuki , Jindo Takashi , Kidokoro Tetsuhiro , Yajima Yoko , Kasuga Junko , Arao Takashi
Published: July 2024

Introduction

With health promotion initiatives in small companies lagging behind those in larger corporations, strengthening health and productivity management in small companies through innovative strategies is an urgent priority. We hypothesized that an outreach strategy involving a public-private partnership would be beneficial for this purpose. The present study examines the implementation of a public-private partnership strategy in Yokohama City, Japan, assessing its impact on health and productivity management in small enterprises, focusing on implementation outcomes.

Methods

As part of the Yokohama Linkworker Project (Y-Link Project), this study describes and examines a public-private partnership program in Yokohama City, Japan, involving the city’s government and a private life insurance company. Trained insurance sales representatives served as “Linkworkers” for the program, reaching out to small enterprises in the city. These Linkworkers provided tailored support to these companies, assisting them with obtaining the “Yokohama Health and Productivity Management Certification” issued by the City of Yokohama authorities and collaborating with external entities to offer health promotion programs for employees. Program interventions took place from August to September 2020. The RE-AIM framework was utilized to evaluate the Project. Data were extracted from Linkworkers’ activity records, certification records, the Linkworker survey, and follow-up surveys with participating companies at 6– and 18– months post-interventions.

Results

Within 2 months, 71 Linkworkers visited 500 companies (50% were small firms, <50 employees). Among them, 224 (45%) enterprises received certifications, contributing to an increased regional certification rate. Linkworker-assisted companies tended to be significantly smaller in size. The odds ratios of implementing workplace health promotion programs in certified firms, compared to non-certified firms, were 4.09 (95% CI: 1.79–9.35) at 6 months and 2.31 (95% CI: 1.04–5.11) at 18 months. For small firms, the odds ratios were 6.87 (95% CI: 1.74–27.06) at 6 months and 3.42 (95% CI: 1.17–10.03) at 18 months. The certification retention rate at 24 months was 60%, irrespective of company size. Linkworkers perceived the outreach strategy as having a positive impact on their primary operations.

Conclusion

The Y-Link Project’s outreach strategy enhanced health and productivity management in small enterprises in Yokohama City, enabling long-term health promotion programs addressing program availability disparities related to company size.
Clinical characteristics and risk factors of Hepatitis E virus infection in Zhejiang Province: a multicenter case–control study
Authors: Chen Yijuan , Gao Jian , Sun Wanwan , Zhu Weiping , Wang Pingping , Ren Xiaobin , Wu Yumeng , Zhang Jianli , Miao Ziping
Published: July 2024

Introduction

Hepatitis E (HE), caused by the Hepatitis E virus (HEV), is a significant cause of acute viral hepatitis globally and a major public health concern, particularly in specific high-prevalence areas in China, which have diverse transmission routes and regional differences. Identifying the primary risk factors for HE transmission is essential to develop targeted interventions for vulnerable populations.

Methods

This study employed a 1:1 matched case–control methodology, using a standardized questionnaire complemented by medical records for data validation.

Results

Among the 442 HE cases and 428 healthy controls, the case group had a higher prevalence of fatigue (46.21%) and loss of appetite (43.84%) compared to the control group. Furthermore, liver function indicators were significantly higher in the case group, with an average alanine aminotransferase (ALT) level of 621.94 U/L and aspartate aminotransferase (AST) level of 411.53 U/L. Severe HE patients were predominantly male, with significantly increased ALT and AST levels reaching 1443.81 U/L and 862.31 U/L respectively, along with a higher incidence of fatigue (90%) and loss of appetite (75%). Multifactorial analysis indicated that frequent dining out (OR = 2.553, 95%CI:1.686–3.868), poor hygiene conditions (OR = 3.889, 95%CI:1.399–10.807), and comorbid chronic illnesses (OR = 2.275, 95%CI:1.616–3.202) were risk factors for HE infection; conversely, good hygiene practices were protective factors against HE infection (OR = 0.698, 95%CI:0.521–0.934).

Conclusion

In conclusion, HE infection in Zhejiang Province is closely associated with dietary habits and environmental hygiene, and individuals with chronic diseases or co-infections are at increased risk. This highlights the need for targeted health education to reduce the incidence of HE among these populations.
Analysis on epidemiological and drug resistance characteristics of lymph node tuberculosis from Hunan province, China
Authors: Fang Tanwei , Xiang Yu , Li Wenbin , Liu Binbin , Liu Haican , Wu Yaning , Xu Da , Li Machao , Yang Shuliu , Li Jixiang , Yu Yanyan , Zhao Xiuqin , Zhao Li-li , Wan Kanglin , Yuan Xiuqin , Tan Yunhong , Li Guilian
Published: July 2024

Objectives

To investigate the clinical epidemiological and drug resistance (DR) characteristics of lymph node tuberculosis (LNTB) in Hunan Province which locates in South-central China, and to provide scientific clues for effective prevention and treatment of LNTB.

Methods

We retrospectively collected LNTB patients with Mycobacterium tuberculosis culture positive at Hunan Chest Hospital, the biggest TB reference hospital in South-central China, from January 2013 to December 2021. The multiple demographic, clinical and drug susceptibility data of patients were collected from the hospital’s electronic patient records. Descriptive statistical methods, Chi-square test and logistic regression analysis were employed as statistical methods.

Results

Of the 577 LNTB cases, 373 (64.64%) were males, 352 (61.01%) were farmers; majority (161, 33.10%) aged at 20–29 years old; 147 (25.48%) had simple LNTB, 350 (60.66%) had LNTB combined with pulmonary TB (PTB) (defined as LNTB-PTB), and 80 (13.86%) had LNTB combined with other extrapulmonary TB (EPTB) (defined as LNTB-EPTB). A total of 345 (59.79%, 345/577) LNTB patients had cervical node infection, and the simple LNTB patients (81.63%, 120/147) had higher proportion of this infection than LNTB-PTB (51.71%, 181/350) and LNTB-EPTB (55.00%, 44/80) (both p values <0.017), respectively. LNTB-EPTB was more inclined to have abdominal tuberculous LNs (20%, 16/80) and at least four tuberculous lesions (22.50%, 18/80) than simple LNTB and LNTB-PTB. Seventy-seven (13.34%) and 119 (20.62%) were resistant to rifampicin (RIF) and isoniazid (INH), respectively; 72 (12.48%) were multi-drug resistant (MDR), and a total of 150 (26.00%) were DR (resistant to at least one of RIF, INH, ethambutol and streptomycin). LNTB patients aged 30–34 and 50–54 years old (compared to those aged <30 years) were independent predictors of RIF resistance (RR) (ORs were 3.47 and 2.83, respectively; 95% CIs were 1.64–7.35 and 1.08–7.46, respectively).

Conclusion

Our study disclosed the epidemiological and DR characteristics of LNTB in Hunan Province, China. High LNTB prevalence was found in younger people while high RR LNTB prevalence was found in older ones, suggesting that we should conduct further studies to clarify the occurrence of RR in LNTB, meanwhile, strengthen the diagnoses and treatments of LNTB to prevent the emergence of RR.
Mixed-methods approach in evaluating safe abortion care services at public health facilities in North Shewa zone, central Ethiopia: a multicenter institutional cross-sectional study
Authors: Tamire Aklilu , Birhanu Bezawit , Negash Abraham , Dechasa Mesay , Masrie Awoke , Shawel Samrawit , Dereje Jerman , Gebru Tilaye , Kassa Tafesse Obsan , Mengistu Dechasa Adare , Sertsu Addisu , Daka Dawit Wolde
Published: July 2024

Background

Of the 55.7 million abortions that were performed globally, 25.1 million (45.1%) were not safe. Nearly 97% of these took place in developing countries. Approximately 71% of economically developed countries allow safe abortion care (SAC) services, whereas only 16% of developing countries permit it. In sub-Saharan Africa, 92% of mothers live in 43 countries where SAC services are restricted by law. Most Ethiopian women continue to self-terminate unwanted pregnancies in hazardous conditions. The aim of this evaluation was to assess input, care providers’ compliance with national guidelines, and clients’ satisfaction.

Methods

A multicenter cross-sectional study design with a mixed-methods approach was used. Seven public health facilities were randomly selected where 75 health caseworkers were directly observed; 296 clients and 14 key informants were interviewed, respectively. A resource inventory checklist was used to assess all inputs. The overall SAC services evaluation was summarized from 40 indicators: 13 resource availability indicators, 14 healthcare workers' compliance to national guidelines indicators, and 13 clients' satisfaction toward SAC services indicators. A multivariate logistic regression model was fit to determine factors that affect client satisfaction at a p-value <0.005.

Results

There were 75 healthcare providers in the maternal and child health departments in the study area. Except for the interruption of water and electricity, maternal waiting area, counseling, and procedural room, all are available making 94% of resources availability. All healthcare workers were compliant in providing anti-pain medication during procedures, identifying clients if they were targeted for an HIV/AIDS test, and providing their test results as per the guideline. Nevertheless, they were poorly compliant in respecting the clients (9, 12%) and taking vital sign (33, 44%). The overall compliance was 62.3%, while only 51% were satisfied with waiting time and privacy of counseling room. The overall client satisfaction was 65%. The overall evaluation of SAC services was 72.9%.

Conclusion

Resource availability was excellent, which was in line with national SAC expectations while the healthcare workers’ compliance to national guidelines was fair, which deviated from expectations. The clients’ satisfaction and the overall evaluation were good, which was below the hypothesized expectation.
Dental caries in children and adolescents with poorly-controlled diabetes: a case-control study
Authors: ElBshari Shahd , Afrooz Imrana , Beck Rasha Hassan , Watad Rama , Al-Qahtani Nabras , Deeb Asma
Published: July 2024

The relationship between diabetes and dental caries remains uncertain. The main objective of this study was to quantify dental caries in children and adolescents with and without poorly-controlled diabetes to examine whether poorly-controlled diabetes influences caries prevalence and severity. This was a case-control study of children and adolescents with poorly-controlled diabetes and age-matched controls attending paediatric clinics at Sheikh Shakhbout Medical City, Abu Dhabi, UAE in August 2022. Dental caries was diagnosed by visual examination and dental probing to derive total number of decayed, missing, and filled tooth or surface (DMFT/DMFS) indices. Differences in caries metrics between subjects with diabetes and controls were assessed using chi-squared or Mann Whitney U-tests. Fifty-seven children and adolescents without diabetes and 42 with poorly-controlled (HbA1c ≥ 7.5) diabetes were recruited. The median (interquartile range, IQR) DMFT index was 4 (5) and the DMFS index was 4 (11). There were no significant differences in DMFT % [14.0 (21.5) vs.13.0 (20.0); p = 0.602], DMFT index [4 (5) vs. 3 (6); p = 0.749], nor DMFS index [5 (12) vs. 4 (11); p = 0.484] between patients and controls. Diabetes either has no effect on caries risk or its effect is so small that it is masked by dominant risk factors such as diet and obesity that require addressing through robust public health measures. While poor glycaemic control does not appear to influence caries risk, diet and obesity remain serious and addressable risk factors affecting oral health.
Validating decarbonisation strategies of climate action plans via digital twins: a Limerick case study
Authors: Byrne Niall , Pierce Susan , De Donatis Lorenzo , Kerrigan Ruth , Buckley Niall
Published: July 2024

The European Green Deal (EGD) represents the response of the European Union (EU) to the climate crisis and a strategy to mitigate against climate change and the associated environmental degradation. European Climate Law supports the EGD by setting the legally binding target of climate neutrality for the EU on a time horizon of 2050 and mandates that member states develop and implement their own Climate Action Plans (CAP), which are strategic roadmaps outlining steps to reduce greenhouse gas (GHG) emissions and adapt to the impacts of climate change. Within the Irish context, the CAP focuses on six specific sectors, three of which, namely the energy grid, the built environment and transportation are considered in this research. This policy proposes the retrofitting of all residential buildings in the country to a B2 energy performance certificate (EPC), all public transport and 60% of private vehicles electrified and the full decarbonisation of the electrical grid by 2050. This study aims to implement the targets and goals outlined within CAP 2021 in an urban area of Ireland, specifically a historical city centre. The research leverages digital twin technology developed by IES to model the carbon emissions associated with the built environment, while also incorporating GHG emissions associated with transportation and the grid. Furthermore, CAP scenarios are applied systematically and strategically, prioritising building retrofits based on buildings with similar characteristics, typologies and performance. This study has assessed the performance of CAP about two future grid scenarios (1) business as usual and (2) a decarbonised grid. In both scenarios, CAP was not sufficient to achieve the targets set out, resulting in a shortfall of between 25 and 33%. Based on this outcome it may be concluded that any decarbonisation targets specified should be tested and validated before their implementation in policy. Moreover, current state-of-the-art digital twin technologies can facilitate the rapid holistic appraisal of complex multi-sectoral policy at the urban scale, which, depending on data availability, could prove useful in future iterations of CAP at both national and international levels.
A two-step strategy to identify episodic sources of gravitational waves and high-energy neutrinos in starburst galaxies
Authors: Allen M. L. , Biermann P. L. , Caramete L. I. , Chieffi A. , Chini R. , Frekers D. , Gergely L. Á. , Harms B. , Jaroschewski I. , Joshi P. S. , Kronberg P. P. , Kun E. , Meli A. , Seo E.-S. , Stanev T.
Published: July 2024

Supermassive black hole (BH) mergers with spin-flips accelerate energetic particles through their relativistic precessing jets, producing high-energy neutrinos and finally gravitational waves (GWs). In star formation, massive stars form in pairs, triplets, and quadruplets, allowing second-generation mergers of the remnants with discrepant spin directions. The GW data support such a scenario. Earlier, we suggested that stellar mass BH mergers (visible in M82) with an associated spin-flip analogously allow the acceleration of energetic particles, with ensuing high-energy neutrinos and high-energy photons, and finally produce GWs. At cosmic distances, only the GWs and the neutrinos remain detectable. In this study, we generalize the argument to starburst and normal galaxies throughout their cosmic evolution and show that these galaxies may dominate over active galactic nuclei (AGN) in the flux of ultra-high-energy particles observed at Earth. All these sources contribute to the cosmic neutrino background, as well as the GW background (they detected lower frequencies). We outline a search strategy to find such episodic sources, which requires including both luminosity and flux density.
Case Report: Minimally invasive repair of a traumatic abdominal wall hernia in a child with a fascial closure device
Authors: Lu Huiyu , Zhang Zemin , Chao Jun , Zhang Chuanguang , Zhang Guoqing , Tao Shujie , Yan Qingtao
Published: July 2024

Abdominal trauma is common in daily life, but a traumatic abdominal wall hernia (TAWH) in children is rare. A TAWH is caused by a huge external force that leads to subcutaneous muscle and fascia rupture, while the skin remains intact. As abdominal pressure increases, the abdominal contents protrude, forming a lump. A TAWH is highly susceptible to missed diagnosis because of other severe injuries. We report a case of a 2-year-old boy with a TAWH who developed a prominent subcutaneous mass on the right side of his abdomen after abdominal trauma; the size of the mass changed significantly with abdominal pressure and crying. In this case, we used a new approach of laparoscopic suture repair technique with the assistance of a fascial closure device and achieved good results. We found that this method offers the advantages of minimally invasive surgery, fast recovery, and no visible surgical incisions. There was no recurrence after 8 months of follow-up.
Fusarium and Hazelnut: A Story of Twists and Turns
Authors: Zimowska Beata , Ludwiczuk Agnieszka , Manganiello Gelsomina , Wojtanowski Krzysztof , Kot Izabela , Staropoli Alessia , Vinale Francesco , Nicoletti Rosario
Published: July 2024

In recent years, the number of reports of Fusarium in association with hazelnut (Corylus avellana) has been increasing worldwide, related to both pathogenic aptitude and endophytic occurrence. However, the assessment of the real ecological role and relevance to plant health of these fungi has been impaired by uncertainty in species identification, deriving from both the evolving taxonomic structure of the genus and an inaccurate use of molecular markers. In this paper, the characterization of two hazelnut endophytic strains isolated in Poland is reported with reference to their secondary metabolite profiles and interactions with pests and pathogens. Our results are indicative of a possible role of these strains in defensive mutualism which could be related to the production of several bioactive compounds, especially cyclohexadepsipeptides of the enniatin family. At the same time, these biochemical properties create some concern for the possible mycotoxin contamination of hazelnut products.
Design of Adaptive Grippers for Fruit-Picking Robots Considering Contact Behavior
Authors: Xie Bowei , Jin Mohui , Duan Jieli , Li Zewei , Wang Weisheng , Qu Mingyu , Yang Zhou
Published: July 2024

Adaptability to unstructured objects and the avoidance of target damage are critical challenges for flexible grippers in fruit-picking robots. Most existing flexible grippers have many problems in terms of control complexity, stability and cost. This paper proposes a flexible finger design method that considers contact behavior. The new approach incorporates topological design of contact targets and introduces contact stress constraints to directly obtain a flexible finger structure with low contact stress and good adaptability. The study explores the effects of design parameters, including virtual spring stiffness, volume fraction, design domain size, and discretization, on the outcomes of the flexible finger topology optimization. Two flexible finger structures were selected for comparative analysis. The experimental results verified the effectiveness of the design method and the maximum contact stress was reduced by about 70%. An adaptive two-finger gripper was developed. This design allows the gripper to achieve damage-free grasping without additional sensors and control systems. The adaptive and contact performances of the grippers with different driving modes were analyzed. Practical grasping tests were also performed, including evaluation of adaptive performance, stability, and maximum grasping weight. The results indicate that gripper 2 with flexible finger 2 excelled in contact stress and adaptive wrapping, making it well-suited for grasping unstructured and fragile objects. This paper provides valuable insights for the design and application of flexible grippers for picking robots, offering a promising solution to enhance adaptability while minimizing target damage.
Customers’ Perception of Microfinance Services as a Tool for Rural Development: A Romanian Case Study
Authors: Henegar Denisa , Ilieș Garofița Loredana , Mureșan Iulia C. , Poruțiu Andra Ramona , Arion Iulia Diana , Arion Felix H.
Published: July 2024

Microfinance institutions (MFIs) play a vital role in extending financial services to marginalized and underprivileged populations worldwide. While the focus of MFIs has traditionally been on providing financial products, recent research highlights the importance of intangible factors in shaping their success and sustainability. This research examines the influence of factors such as trust, empathy, organizational culture, and reputation on MFI performance. A structured questionnaire was developed, and data were collected from 110 clients. Statistical analysis, including Cronbach alpha, composite reliability, and exploratory factor analysis, was employed to assess the reliability, validity, and dimensionality of the collected data. Three dimensions of service quality were identified: empathy and assurance, trust, and intangibles. While gender differences in perception were observed, they were not statistically significant. However, significant differences were noted across age groups, educational levels, and types of businesses. Understanding and addressing factors related to trust, intangibles, and specific aspects of service satisfaction are crucial for enhancing client engagement and long-term success. By continuously striving to improve service quality, microfinance companies can strengthen client relationships and position themselves in such a way as to achieve sustainable impact and success in the microfinance landscape.
Can Rural Digitization and the Efficiency of Agricultural Carbon Emissions Be Coupled and Harmonized under the “Dual-Carbon” Goal?
Authors: Jin Mingming , Wang Shuokai , Chen Ni , Feng Yong , Cao Fangping
Published: July 2024

A major driving force behind China’s low-carbon, environmentally friendly development of agriculture and the accomplishment of the “dual-carbon” goal is the digital transformation of rural areas. In this study, on the basis of clarifying the degree of rural digitization and agricultural carbon emissions efficiency in China from 2010 to 2021, the degree of coupled coordination and the spatiotemporal pattern characteristics between the two are examined using the coupled coordination model. Then, the influencing factors are analyzed in depth using the spatial Durbin model. Our findings reveal that, first, in terms of the degree of rural digitization, the index increases overall and the spatial imbalance is obvious, with a spatial distribution pattern of “high in the east and low in the west”. Regarding the efficiency of agricultural carbon emissions, there is an overall “N-shaped” change, which is mainly influenced by technological progress in agricultural production, and the regional annual averages are, in descending order, the Western, Eastern, Northeastern, and Central regions, with obvious regional differences. Second, the coupling coordination index shows a fluctuating upward trend, from “extreme disorder” to “high-level coordination”. Furthermore, there are obvious regional characteristics. The regional growth rates are, in descending order, the Western, Eastern, Central, and Northeastern regions. Third, coupling coordination is jointly influenced by a variety of factors, including government input, educational level, industrial structure, energy use, urbanization rate, living standards, driving temporal patterns, and regional differences. This study not only helps to clarify the relationship between the two, offering a reference for the realization of the “dual-carbon” goal, but also broadens the concepts of the low-carbon and environmentally friendly development of agriculture.
Schooling Fish from a New, Multimodal Sensory Perspective
Authors: Larsson Matz
Published: July 2024

The acoustic hypothesis suggests that schooling can result in several benefits. (1) The acoustic pattern (AP) (pressure waves and other water movements) produced by swimming are likely to serve as signals within fish shoals, communicating useful spatial and temporal information between school members, enabling synchronized locomotion and influencing join, stay or leave decisions and shoal assortment. (2) Schooling is likely to reduce the masking of environmental signals, e.g., by auditory grouping, and fish may achieve windows of silence by simultaneously stopping their movements. (3) A solitary swimming fish produces an uncomplicated AP that will give a nearby predator’s lateral line organ (LLO) excellent information, but, if extra fish join, they will produce increasingly complex and indecipherable APs. (4) Fishes swimming close to one another will also blur the electrosensory system (ESS) of predators. Since predators use multimodal information, and since information from the LLO and the ESS is more important than vision in many situations, schooling fish may acquire increased survival by confusing these sensory systems. The combined effects of such predator confusion and other acoustical benefits may contribute to why schooling became an adaptive success. A model encompassing the complex effects of synchronized group locomotion on LLO and ESS perception might increase the understanding of schooling behavior.
The Welfare of Dogs as an Aspect of the Human–Dog Bond: A Scoping Review
Authors: Verbeek Peter , Majure Chase Alan , Quattrochi Laura , Turner Stephen James
Published: July 2024

The close bond that can exist between humans and their dogs is an important aspect of the evolutionary, economic, and social connections between the two species. There is a need for a better understanding of the place of the dog within the human–dog bond and on ways the human–dog bond affects dog welfare. We conducted a scoping review to investigate to what extent and in what ways dog welfare is addressed in the research literature on the human–dog bond. We identified 706 publications on the human–dog bond from across the globe that were published from 2012 to 2023. We found that 246 of these 706 publications had a focus on dog welfare. Our review showed that the interplay of characteristics and backgrounds of owners/handlers and their dogs was linked to dog welfare in multiple, both positive and negative, ways. Our review is limited by the fact that most of the research that we reviewed involved pet dogs and in majority came from Western, Educated, Industrialized, Rich, Democratic (WEIRD) societies. There is a need for a better understanding of how the human–dog bond affects the welfare of working, assistance, and service dogs.
Influence of Fermented Broccoli Residues on Fattening Performance, Nutrient Utilization, and Meat Properties of Finishing Pigs
Authors: Zhao Zhiwei , Wu Jie , Yao Xiaohong , Sun Hong , Wu Yifei , Zhou Hanghai , Wang Xin , Guo Kai , Deng Bo , Tang Jiangwu
Published: July 2024

The study determined the impacts of dietary fermented residues’ (FBR) inclusion on growth, nutrient utilization, carcass characteristics, and meat properties in fattening pigs. Seventy-two robust pigs were randomly assigned to two experimental groups (Duroc × Landrace × Yorkshire, thirty-six pigs each). Each group was subjected to a 52-day trial, during which they received either a corn–soybean meal-based diet or diet enhanced with a 10% addition of FBR. Consequently, adding 10% FBR caused a significant decrease in the digestive utilization of crude dietary components in fattening pigs (p < 0.05) but showed no significant impact on the growth performance. Additionally, FBR inclusion increased the marbling scores (p < 0.05) and total antioxidant functions (p < 0.05) of muscle tissues, indicating improved meat quality. Gender affected backfat depth, with barrows showing thicker backfat depth. In conclusion, dietary supplementation with 10% FBR in finishing pigs influenced the meat quality by improving the marbling score and antioxidant performance while reducing digestibility without compromising growth performance.
Lack of Efficacy of Albendazole against Dicrocoelium dendriticum Infection in a Sheep Farm in France
Authors: Petermann Julie , Grisez Christelle , Lavigne Sophie , Jacquiet Philippe
Published: July 2024

Dicrocoeliosis is a common parasitic disease in European sheep farming. The prevalence of infection by this parasite can reach almost 70% in areas where the environment is favorable to intermediate hosts. In France, only one drug is currently available for the treatment of dicrocoeliosis: albendazole at a dose of 15 mg/kg in a single administration. However, a control coproscopy following a routine treatment led us to suspect that the efficacy of albendazole against Dicrocoelium dendriticum had diminished. Therefore, we carried out an efficacy test on 15 animals by treating them with albendazole at a dose of 15 mg/kg and performing a coproscopy on D0 and a control coproscopy 14 days later. We obtained a 39% reduction in the excretion of D. dendriticum eggs. This shows a reduction in the expected efficacy of albendazole, which is normally more than 90% in other studies involving this molecule at a dosage of 15 mg/kg. These results are of major concern as albendazole is currently the only drug available in France to treat dicrocoeliosis.
Coatings Based on Essential Oils for Combating Antibiotic Resistance
Authors: Visan Anita Ioana , Negut Irina
Published: July 2024

In the current era of widespread antimicrobial resistance, the utilization of essential oils (EOs) derived from plants has emerged as a promising alternative in combating pathogens that have developed resistance to antibiotics. This review explores the therapeutic potential of essential oils as valuable tools in restoring the efficacy of antibiotics, highlighting their unique ability to affect bacteria in multiple ways and target various cellular systems. Despite the challenge of elucidating their precise mode of action, EOs have shown remarkable results in rigorous testing against a diverse range of bacteria. This review explores the multifaceted role of EOs in combating bacterial microorganisms, emphasizing their extraction methods, mechanisms of action, and comparative efficacy against synthetic antibiotics. Key findings underscore the unique strategies EOs deploy to counter bacteria, highlighting significant differences from conventional antibiotics. The review extends to advanced coating solutions for medical devices, exploring the integration of EO formulations into these coatings. Challenges in developing effective EO coatings are addressed, along with various innovative approaches for their implementation. An evaluation of these EO coatings reveals their potential as formidable alternatives to traditional antibacterial agents in medical device applications. This renaissance in exploring natural remedies emphasizes the need to combine traditional wisdom with modern scientific advancements to address the urgent need for effective antimicrobial solutions in the post-antibiotic era.
Antibiotic Resistance in Acetic Acid Bacteria Originating from Vinegar
Authors: Kim Sun-Hee , Jang Hyun-Wook , Park Jin-Ju , Nam Dong-Geon , Lee Su-Jeong , Yeo Soo-Hwan , Kim So-Young
Published: July 2024

Acetic acid bacteria (AAB) are major contributors to the production of fermented vinegar, offering various cultural, culinary, and health benefits. Although the residual unpasteurized AAB after vinegar production are not pathogens, these are necessary and require safety evaluations, including antibiotic resistance, before use as a starter. In this research, we investigated the antibiotic resistance profiles of 26 AAB strains, including various species of Komagataeibacter and Acetobacter, against 10 different antibiotics using the E-test method. All strains exhibited resistance to aztreonam and clindamycin. Komagataeibacter species demonstrated a 50% resistance rate to ciprofloxacin, analogous to Acetobacter species, but showed twice the resistance rates to chloramphenicol and erythromycin. Genomic analysis of K. saccharivorans CV1 identified intrinsic resistance mechanisms, such as multidrug efflux pumps, thereby enhancing our understanding of antibiotic resistance in acetic acid-producing bacteria. These findings enhance understanding of antibiotic resistance in AAB for food safety and new antimicrobial strategies, suggesting the need for standardized testing methods and molecular genetic study.
Development of a Distilled Beverage Using Yacon Root (Smallanthus sonchifolius)
Authors: Marin Arévalo Manuel José , Ratkovich Nicolás
Published: July 2024

Yacon, known for its fructooligosaccharides, fructans, and inulin content, has shown potential for novel beverage production. This study explores the feasibility of creating a distilled yacon-based beverage. Hydrolysis was utilized to release simple sugars from agave and yacon roots; these were then processed into three distinct batches of distilled beverage. The different methods led to tests varying the sugar content, yeast strains for fermentation, distillation efficiency, aging processes, and sensory evaluations. The distilled beverages demonstrated varying fermentation yields and distillation efficiencies, with one batch aged in Colombian white oak and the others in glass, highlighting differences in flavor profiles. The study concluded that yacon could serve as a versatile base for alcoholic beverage production. The second batch of the distilled beverage, optimized for fermentation and distillation efficiencies, represented promising advancements in yacon-based alcohol production. Future research should focus on process optimization and commercial viability to expand yacon’s presence in the alcoholic beverage industry.
Impact of the COVID-19 Pandemic on Prostate Cancer Diagnosis, Staging, and Treatment: A Population-Based Study in Northern Italy
Authors: Mangone Lucia , Marinelli Francesco , Bisceglia Isabella , Filice Angelina , Braghiroli Maria Barbara , Roncaglia Francesca , Palicelli Andrea , Morabito Fortunato , Neri Antonino , Sabbatini Roberto , Iotti Cinzia , Pinto Carmine
Published: July 2024

The COVID-19 pandemic has caused delays in cancer diagnoses and reductions in treatments. The aim of this work is to evaluate the impact of the pandemic on prostate cancer by evaluating whether there has been a shift towards more aggressive (Gleason) and more advanced tumors (stage IV) and a decline in treatments. The study was conducted on 1123 cases of prostate cancer incident in the Province of Reggio Emilia, Northern Italy, in the period of 2018–2021. In 2020, there was a decline in new diagnoses of prostate cancer (−31%), followed by a slight recovery in 2021 (+5%). While Gleason 7 and 8–10 values remained constant, a significant decrease was recorded in stage I (38.7%, 41.6%, 35.5%, and 27.7%) and an increase in stage IV (13.1%, 13%, 15.4%, and 20%) cases in the years 2018, 2019, 2020, and 2021, respectively. However, there was no impact on surgical treatment (which remained constant at around 35%) and radiotherapy (around 39%). Our findings underline the profound impact of COVID-19 on prostate cancer management, highlighting the importance of healthcare resilience in the face of unprecedented disruptions.
Unusual Patterns of Lateral Scutes in Two Olive Ridley Turtles and Their Genetic Assignment to the Thai Andaman Sea Populations of Lepidochelys olivacea Eschscholtz, 1829
Authors: Kaewmong Patcharaporn , Kittiwattanawong Kongkiat , Nganvongpanit Korakot , Piboon Promporn
Published: July 2024

Two stranded Lepidochelys-like sea turtles were rescued from the Thai Andaman Sea coastline by veterinarians of the Phuket Marine Biological Center (PMBC), one in May of 2019 and another in July of 2021. They were first identified as olive ridley turtles (Lepidochelys olivacea), as the external appearance of both turtles was closer to that species than the other four species found in the Thai Andaman Sea. In fact, when carefully examined, an unusual pattern of the lateral scutes on each turtle was observed, specifically symmetric 5/5 and asymmetric 5/6, both of which are considered rare for L. olivacea and had never been reported in the Thai Andaman Sea. In contrast, this characteristic was more common for the closely related species, Kemp’s ridley (L. kempii), although this species is not distributed in the Indo-Pacific Ocean. Thus, we further investigated their genetic information to confirm species identification using two molecular markers, namely the mtDNA control region and nDNA RAG2. The results from the mtDNA control region sequences using the Basic Local Alignment Search Tool (BLAST) indicated that both individuals exhibited a higher percent identity with L. olivacea (99.81–100.00%) rather than L. kempii (94.29–95.41%) or any other species. A phylogenetic tree confirmed that these two turtles belonged to the L. olivacea clade. Moreover, the results of RAG2 also supported the mtDNA result, as both individuals shared the same RAG2 haplotype with L. olivacea. Thus, we have concluded that the two turtles with unusual lateral scute patterns exhibited genetic consistency with their original species, L. olivacea, which has brought attention to the importance of exploring rare phenotypes in sea turtle populations residing in Thai Seas.
Anti-Inflammatory and Cancer-Preventive Potential of Chamomile (Matricaria chamomilla L.): A Comprehensive In Silico and In Vitro Study
Authors: Drif Assia I. , Yücer Rümeysa , Damiescu Roxana , Ali Nadeen T. , Abu Hagar Tobias H. , Avula Bharati , Khan Ikhlas A. , Efferth Thomas
Published: July 2024

Background and aim: Chamomile tea, renowned for its exquisite taste, has been appreciated for centuries not only for its flavor but also for its myriad health benefits. In this study, we investigated the preventive potential of chamomile (Matricaria chamomilla L.) towards cancer by focusing on its anti-inflammatory activity. Methods and results: A virtual drug screening of 212 phytochemicals from chamomile revealed β-amyrin, β-eudesmol, β-sitosterol, apigenin, daucosterol, and myricetin as potent NF-κB inhibitors. The in silico results were verified through microscale thermophoresis, reporter cell line experiments, and flow cytometric determination of reactive oxygen species and mitochondrial membrane potential. An oncobiogram generated through comparison of 91 anticancer agents with known modes of action using the NCI tumor cell line panel revealed significant relationships of cytotoxic chamomile compounds, lupeol, and quercetin to microtubule inhibitors. This hypothesis was verified by confocal microscopy using α-tubulin-GFP-transfected U2OS cells and molecular docking of lupeol and quercetin to tubulins. Both compounds induced G2/M cell cycle arrest and necrosis rather than apoptosis. Interestingly, lupeol and quercetin were not involved in major mechanisms of resistance to established anticancer drugs (ABC transporters, TP53, or EGFR). Performing hierarchical cluster analyses of proteomic expression data of the NCI cell line panel identified two sets of 40 proteins determining sensitivity and resistance to lupeol and quercetin, further pointing to the multi-specific nature of chamomile compounds. Furthermore, lupeol, quercetin, and β-amyrin inhibited the mRNA expression of the proinflammatory cytokines IL-1β and IL6 in NF-κB reporter cells (HEK-Blue Null1). Moreover, Kaplan–Meier-based survival analyses with NF-κB as the target protein of these compounds were performed by mining the TCGA-based KM-Plotter repository with 7489 cancer patients. Renal clear cell carcinomas (grade 3, low mutational rate, low neoantigen load) were significantly associated with shorter survival of patients, indicating that these subgroups of tumors might benefit from NF-κB inhibition by chamomile compounds. Conclusion: This study revealed the potential of chamomile, positioning it as a promising preventive agent against inflammation and cancer. Further research and clinical studies are recommended.
Evidence-Based Severity Assessment of Animal Models for Pancreatic Cancer
Authors: Schreiber Tim , Koopmann Ingo , Brandstetter Jakob , Talbot Steven R. , Goldstein Lea , Hoffmann Lisa , Schildt Anna , Joksch Markus , Krause Bernd , Jaster Robert , Palme Rupert , Zechner Dietmar , Vollmar Brigitte , Kumstel Simone
Published: July 2024

Animal models are crucial to preclinical oncological research and drug development. Animal experiments must be performed in accordance with the 3R principles of replacement and reduction, if possible, and refinement where these procedures remain crucial. In addition, European Union legislations demand a continuous refinement approach, as well as pro- and retrospective severity assessment. In this study, an objective databased severity assessment was performed in murine models for pancreatic cancer induced by orthotopic, subcutaneous, or intravenous injection of Panc02 cells. Parameters such as body weight change, distress score, perianal temperature, mouse grimace scale, burrowing, nesting behavior, and the concentration of corticosterone in plasma and its metabolites in feces were monitored during tumor progression. The most important parameters were combined into a score and mapped against a reference data set by the Relative Severity Assessment procedure (RELSA) to obtain the maximum achieved severity for each animal (RELSAmax). This scoring revealed a significantly higher RELSAmax for the orthotopic model than for the subcutaneous and intravenous models. However, compared to animal models such as pancreatitis and bile duct ligation, the pancreatic cancer models are shown to be less severe. Data-based animal welfare assessment proved to be a valuable tool for comparing the severity of differently induced cancer models.