bluuebunny/crossref_metadata_2025 huggingface dataset:
Sebetralstat: A Rapidly Acting Oral Plasma Kallikrein Inhibitor for the On-Demand Treatment of Hereditary Angioedema
Authors: Feener Edward P. , Davie Rebecca L. , Murugesan Nivetha , Pethen Stephen J. , Hampton Sally L. , Smith Michael D. , Audhya Paul K. , Yea Chris M.
Published: April 2024

Sebetralstat is a novel, potent, and selective oral plasma kallikrein inhibitor drug candidate in clinical development for the on-demand treatment of hereditary angioedema (HAE). Upon binding, sebetralstat induces a conformational change in the active site of plasma kallikrein, which contributes to its high potency (Ki 3 nM) and selectivity (>1500 fold) against other serine proteases. Its physiochemical properties promote both rapid dissolution in the stomach and rapid absorption in the upper intestine that contribute to its fast and efficient absorption. A single oral administration of sebetralstat rapidly provides near-complete inhibition of plasma kallikrein and blockade of high-molecular-weight kininogen cleavage as early as 15 min, which drives its clinical efficacy. In a phase 2 clinical trial, sebetralstat significantly reduced the time to beginning of symptom relief (p < 0.0001) with median times of 1.6 h (95% CI: 1.5–3.0) with sebetralstat versus 9.0 h (4.0–17.2) with placebo. KONFIDENT (NCT05259917) is a phase 3 clinical trial assessing the on-demand use of sebetralstat for HAE. If successful, this trial could support the approval of sebetralstat as the first noninvasive, on-demand treatment option to rapidly halt HAE attacks and provide fast symptom relief.
Common Nutritional Shortcomings in Vegetarians and Vegans
Authors: Gibbs Joshua , Cappuccio Francesco P.
Published: April 2024

The popularity of vegetarian and vegan diets is growing due to increased awareness of the environmental and health benefits of such diets. However, despite the consumption of meatless diets being associated with decreased risk of chronic diseases and mortality, followers of these diets are prone to certain nutritional inadequacies, which could limit health benefits. The main nutrients of concern are vitamin B12, vitamin D, iodine, selenium, calcium, and iron. It is essential for all those following vegetarian and vegan diets to implement vitamin B12 supplementation. To prevent vitamin B12 deficiency, adults should take one oral dose of 50–100 μg cyanocobalamin daily or 2000 μg weekly divided into two oral cyanocobalamin doses. Iodine supplementation is essential for pregnant and breastfeeding women and is recommended for vegetarians and vegans who are unable to access sea vegetables or iodine-fortified foods. The recommended dose of iodine supplement for adults is 150 μg daily. Selenium supplementation should be reserved for individuals with clinical evidence of deficiency due to concerns of adverse effects on lipid profiles and type 2 diabetes risk. It is recommended that vegetarian and vegan adults should supplement 4000 International Units (IU)/d of oral vitamin D to prevent deficiency as sunlight exposure is unreliable due to a range of lifestyle and environmental factors. This recommendation also applies to omnivores. As a precaution, those wishing to transition to a more plant-based diet should consider how they will substitute the nutrition they currently obtain from animal-based foods with plant-based sources or appropriate supplementation.
On the Fidelity of RANS-Based Turbulence Models in Modeling the Laminar Separation Bubble and Ice-Induced Separation Bubble at Low Reynolds Numbers on Unmanned Aerial Vehicle Airfoil
Authors: Muhammed Manaf , Virk Muhammad Shakeel
Published: April 2024

The operational regime of Unmanned Aerial Vehicles (UAVs) is distinguished by the dominance of laminar flow and the flow field is characterized by the appearance of Laminar Separation Bubbles (LSBs). Ice accretion on the leading side of the airfoil leads to the formation of an Ice-induced Separation Bubble (ISB). These separation bubbles have a considerable influence on the pressure, heat flux, and shear stress distribution on the surface of airfoils and can affect the prediction of aerodynamic coefficients. Therefore, it is necessary to capture these separation bubbles in the numerical simulations. Previous studies have shown that these bubbles can be modeled successfully using the Large Eddy Simulation (LES) and Direct Numerical Simulation (DNS) but are computationally costly. Also, for numerical modeling of ice accretion, the flow field needs to be recomputed at specific intervals, thus making LES and DNS unsuitable for ice accretion simulations. Thus, it is necessary to come up with a Reynolds-Averaged Navier–Stokes (RANS) equation-based model that can predict the LSBs and ISBs as accurately as possible. Numerical studies were performed to assess the fidelity of various RANS turbulence models in predicting LSBs and ISBs. The findings are compared with the experimental and LES data available in the literature. The structure of these bubbles is only studied from a pressure coefficient perspective, so an attempt is made in these studies to explain it using the skin friction coefficient distribution. The results indicate the importance of the use of transition-based models when dealing with low-Reynolds-number applications that involve LSB. ISB can be predicted by conventional RANS models but are subjected to high levels of uncertainty. Possible recommendations were made with respect to turbulence models when dealing with flows involving LSBs and ISBs, especially for ice accretion simulations.
High-Rate Performance of a Designed Si Nanoparticle–Graphite Nanosheet Composite as the Anode for Lithium-Ion Batteries
Authors: Ghanooni Ahmadabadi Vahide , Rahman Md Mokhlesur , Chen Ying
Published: April 2024

A silicon nanoparticle–graphite nanosheet composite was prepared via a facile ball milling process for use as the anode for high-rate lithium-ion batteries. The size effect of Si nanoparticles on the structure and on the lithium-ion battery performance of the composite is evaluated. SEM and TEM analyses show a structural alteration of the composites from Si nanoparticle-surrounded graphite nanosheets to Si nanoparticle-embedded graphite nanosheets by decreasing the size of Si nanoparticles from 250 nm to 40 nm. The composites with finer Si nanoparticles provide an effective nanostructure containing encapsulated Si and free space. This structure facilitates the indirect exposure of Si to electrolyte and Si expansion during cycling, which leads to a stable solid–electrolyte interphase and elevated conductivity. An enhanced rate capability was obtained for the 40 nm Si nanoparticle–graphite nanosheet composite, delivering a specific capacity of 276 mAh g−1 at a current density of 1 C after 1000 cycles and a rate capacity of 205 mAh g−1 at 8 C.
SDEWES 2023: Barriers and Possibilities for the Development of Short-Rotation Coppice as an Agroforestry System for Adaptation to Climate Change in Central European Conditions
Authors: Weger Jan , Vávrová Kamila , Janota Lukáš , Knápek Jaroslav
Published: April 2024

This article compares different production, economic, and selected environmental aspects of agroforestry systems in a standard (alley cropping) and a newly proposed design with fast-growing trees grown in short-rotation coppice. Our models of agroforestry systems (AFSs) are as follows: (i) alley cropping AFS with cherry and walnut trees in single rows (tree strips) with 28 m-wide arable fields between them (crop strips), and (ii) coppiced tree belt AFS with poplars and willows and 25 m-wide arable fields between them (crop strips). To evaluate the production characteristics of trees, we used yield curves from experimental plantations in conditions of the Czech Republic from previous research projects. Cost data were collected from long-term experimental plantations and combined with current operation and energy prices. The article presents an economic methodology for assessing the competitiveness of biomass production in AFSs under the current identified market conditions. Our results show that AFSs with short-rotation coppice can have similar economic and production results as annual crops if grown on suitable sites and with appropriate quality of agronomy. In comparison, alley cropping AFSs with fruit trees would not be economically viable for farmers without a significant subsidy for establishment and maintenance in the first years after establishment. Concerning the latest economic and political developments, the product from SRC (energy woodchips) can be evaluated as strategic, increasing the producer’s independence from purchased energy fuels.
A Model Modification for a Microturbine Set with Partial Admission Stages
Authors: Włodarski Wojciech , Piwowarski Marian
Published: April 2024

The vapour microturbine set’s mathematical model has been updated to consider the partial admission of turbine stages. Experimental data from two distinct microturbine sets were used to verify the model. The model of the microturbine set was tested under varying operating conditions. Examples of a comparison between the experimental results and simulations are presented and analysed. It has been shown that, when simulating the off-design operation of multistage microturbines, not taking into account power losses due to partial admission may lead to significantly incorrect results. This conclusion does not apply to single-stage microturbines.
Energy Harnessing Performance of Oscillating Foil Submerged in the Wake of a Fixed Cylinder
Authors: Luo Yongqing , Wu Houxian , Huang Shuhan , Sun Hai
Published: April 2024

The energy harnessing from flow-induced vibrations (FIV) by an oscillating foil placed tandemly behind a circular cylinder (which serves as a vortex generator) is investigated. The foil is submerged in the wake produced by the fixed cylinder and could oscillate in the direction perpendicular to the incoming flow with single-degree freedom. The spacing ratio ranges from 1.0 to 5.0. The oncoming fluid velocity is U = 1–10 m/s, corresponding to the reduced velocity Ur = 3.81–38.08 and the Reynolds number Re = 9.58 × 103–9.58 × 104. Four harnessing damping ratios (ζharness = 0.0054–0.0216) are used to simulate the energy conversion conditions. The main conclusions are: (1) The optimal oscillation pattern related to the highest harnessed energy emerges as the spacing ratio close to 1.0. (2) The airflow energy converted by the foil is positively correlated with the harnessing damping ratio because the amplitude responses are similar at various harnessing damping ratios. A high velocity yields the highest harnessed power. (3) The harnessing efficiency of the foil could reach 48.89%, which is much more than that of an isolated flapping foil.
Surfactant-Enhanced Assisted Spontaneous Imbibition for Enhancing Oil Recovery in Tight Oil Reservoirs: Experimental Investigation of Surfactant Types, Concentrations, and Temperature Impact
Authors: Wang Fuyong , Hua Haojie , Wang Lu
Published: April 2024

Surfactant-assisted spontaneous imbibition is an important mechanism in enhanced oil recovery by capillary pressure in low permeability and tight oil reservoirs. Though many experiments have been conducted to study the mechanism of enhanced oil recovery by surfactant-assisted spontaneous imbibition, the effects of surfactant type, concentration, and temperature have not been well studied. Using tight sandstone outcrop core samples with similar permeability and porosity, this paper experimentally studies surfactant-assisted spontaneous imbibition using three different surfactant types, i.e., sodium dodecylbenzene sulfonate (SDBS), cocamidopropyl betaine (CAB), and C12–14 fatty alcohol glycoside (APG). In addition to the type of surfactant, the effect of the surfactant concentration and the temperature is also investigated. The study results show that the ultimate oil recovery of spontaneous imbibition with formation water and denoised water is about 10%. Surfactant can significantly improve the oil recovery of spontaneous imbibition by reducing the interfacial tension between oil and water, emulsifying crude oil and improving oil mobility. APG showed better performance compared to SDBS and CAB, with a maximum oil recovery factor of 36.19% achieved with formation water containing 0.05% APG surfactant. Lower concentrations (0.05% APG) in the formation water resulted in a higher oil recovery factor compared to 0.1% APG. Increasing temperature also improves oil recovery by reducing oil viscosity. This empirical study contributes to a better understanding of the mechanism of surfactant-assisted spontaneous imbibition and enhanced oil recovery in tight oil reservoirs.
Nearest Vector Control Method Applied to an MMC for PV Generation
Authors: Bakir Yad N. , de Pablo Santiago , Martinez-Rodrigo Fernando , Aljawary Zaid A. , Herrero-de Lucas Luis Carlos
Published: April 2024

This paper proposes a new and simplified Nearest Vector Control (NVC) modulation technique for a grid-connected photovoltaic (PV) system using a Modular Multilevel Converter (MMC). Compared to the Nearest Level Control (NLC) technique, which defines three independent states for the three phases of medium to large four-wire multilevel converters, NVC offers a more coordinated behavior for three-wire converters. The proposed scheme is easy to implement, and it simplifies the understanding of using vectors when detecting the vector of the converter nearest to a given reference. Because it uses natural coordinates, namely, ab, bc and ca, the proposed method is easier to understand and more useful for further developments. Compared with earlier NVC methods, this approach offers full independence of the number of levels at the converter and it can readily accommodate changes in the number of levels, with no need for lookup tables or artificial coordinate transformations. The proposed NVC method was implemented on a 16-cell MMC used for PV generation and then it was compared to NLC, leading to a smaller and more consistent low-order harmonic distortion, requiring about the same complexity of implementation. Furthermore, in comparison to NLC, when applying the proposed NVC modulation, a behavior more insensitive to changes in the grid voltage was found, the most hazardous odd harmonics from the 5th to the 19th were reduced, and a consistent reduction of about 25 dB was achieved on the 5th and 7th harmonics. The newly proposed method is supported by simulations and experimental results with constant and sharply changing solar irradiance, leaving or removing the 100 Hz component of the MMC circulating currents.
Improved YOLOv5 Based on Multi-Strategy Integration for Multi-Category Wind Turbine Surface Defect Detection
Authors: Lei Mingwei , Wang Xingfen , Wang Meihua , Cheng Yitao
Published: April 2024

Wind energy is a renewable resource with abundant reserves, and its sustainable development and utilization are crucial. The components of wind turbines, particularly the blades and various surfaces, require meticulous defect detection and maintenance due to their significance. The operational status of wind turbine generators directly impacts the efficiency and safe operation of wind farms. Traditional surface defect detection methods for wind turbines often involve manual operations, which suffer from issues such as high subjectivity, elevated risks, low accuracy, and inefficiency. The emergence of computer vision technologies based on deep learning has provided a novel approach to surface defect detection in wind turbines. However, existing datasets designed for wind turbine surface defects exhibit overall category scarcity and an imbalance in samples between categories. The algorithms designed face challenges, with low detection rates for small samples. Hence, this study first constructs a benchmark dataset for wind turbine surface defects comprising seven categories that encompass all common surface defects. Simultaneously, a wind turbine surface defect detection algorithm based on improved YOLOv5 is designed. Initially, a multi-scale copy-paste data augmentation method is proposed, introducing scale factors to randomly resize the bounding boxes before copy-pasting. This alleviates sample imbalances and significantly enhances the algorithm’s detection capabilities for targets of different sizes. Subsequently, a dynamic label assignment strategy based on the Hungarian algorithm is introduced that calculates the matching costs by weighing different losses, enhancing the network’s ability to learn positive and negative samples. To address overfitting and misrecognition resulting from strong data augmentation, a two-stage progressive training method is proposed, aiding the model’s natural convergence and improving generalization performance. Furthermore, a multi-scenario negative-sample-guided learning method is introduced that involves incorporating unlabeled background images from various scenarios into training, guiding the model to learn negative samples and reducing misrecognition. Finally, slicing-aided hyper inference is introduced, facilitating large-scale inference for wind turbine surface defects in actual industrial scenarios. The improved algorithm demonstrates a 3.1% increase in the mean average precision (mAP) on the custom dataset, achieving 95.7% accuracy in mAP_50 (the IoU threshold is half of the mAP). Notably, the mAPs for small, medium, and large targets increase by 18.6%, 16.4%, and 6.8%, respectively. The experimental results indicate that the enhanced algorithm exhibits high detection accuracy, providing a new and more efficient solution for the field of wind turbine surface defect detection.
The Feasibility Study of In Situ Conversion of Oil Shale Based on Calcium-Oxide-Based Composite Materia Hydration Exothermic Reaction
Authors: Ma Shiwei , Li Shouding , Zhang Zhaobin , Xu Tao , Zheng Bo , Hu Yanzhi , Li Guanfang , Li Xiao
Published: April 2024

Oil shale, as a vast potential resource, is considered an important alternative energy source, and its effective development and economic utilization are of significant importance in alleviating the contradiction between energy supply and demand. Presently, the in situ conversion technology for oil shale has gained significant global attention, with numerous extraction methods undergoing active research and development. One of these methods is the in situ conversion of oil shale based on the hydration reaction of calcium-oxide-based composite material (CaO-CM). This approach harnesses the heat produced by the reaction between CaO-CM and water as a heat source for the pyrolysis of oil shale. This paper conducted experiments to assess the feasibility of temperature associated with this method. The feasibility study mainly includes two aspects: First, it is necessary to investigate whether the temperature generated by the hydration reaction of CaO-CM can meet the temperature requirements for the pyrolysis of oil shale. Through pyrolysis experiments of Xinjiang oil shale, the minimum temperature required for oil shale pyrolysis was determined to be 330 °C. High-temperature and high-pressure reaction vessels were employed to explore the temperature generated by the hydration reaction of CaO-CM. The results show that with the increase in environment pressure, environment temperature, and reaction mass, the maximum temperature generated by the hydration reaction of CaO-CM also increases (reach 455.5 °C), meeting the temperature requirements for the pyrolysis of oil shale. Second, the study evaluates whether the hydration reaction of CaO-CM can induce pyrolysis hydrocarbons of the oil shale. Through the pyrolysis experiments of oil shale based on the hydration reaction of CaO-CM, the changes in the content of pyrolysis hydrocarbons (S2) in oil shale before and after pyrolysis are measured. The results show that under 10 MPa pressure, the content of pyrolysis hydrocarbons in the oil shale decreased from 40.96 mg/g to 0.08 mg/g after pyrolysis. This confirms the feasibility of the temperature conditions for the in situ conversion of oil shale based on the hydration reaction of CaO-CM.
A Bi-Level Optimal Scheduling Strategy for Microgrids for Temperature-Controlled Capacity and Time-Shifted Capacity, Considering Customer Satisfaction
Authors: Yang Yulong , Zhang Zhiwei
Published: April 2024

Since microgrids can effectively integrate renewable energy, energy storage devices, and controllable loads, this advantage promotes the rapid development and application of microgrid technology. However, with the high proportion of renewable energy access, only considering how energy is optimally distributed in microgrids can no longer meet the actual demand. How to aggregate user-side controllable loads to form regulation resources has become a research hotspot, and the users, as a passive party in the load scheduling process, should also be an important consideration in their perception of the use of electricity. First, a control model for temperature-controlled loads and a time-shift model for time-shiftable loads are developed. Then, the comprehensive electricity satisfaction model of users is established, and the two-layer optimal scheduling model of microgrids considering users’ satisfaction is proposed, with users as the upper layer and microgrids as the lower layer, and the two-layer model is transformed into a single-layer model according to the KKT condition for solving. Finally, the effect of the weighting factor for satisfaction on the economy is discussed through the analysis of examples, which verifies the effectiveness of the two-layer model.
Evaluation of Antidiabetic Potential of Mangifera indica Leaf in Streptozotocin-Induced Type 2 Diabetic Rats: Focus on Glycemic Control and Cholesterol Regulation
Authors: Khan Joyeeta T. , Richi Anika E. , Riju Sanjida A. , Jalal Tanjila , Orchi Rejwana J. , Singh Smita , Bhagat Phulgen , Abdel-Wahab Yasser H. A. , Ansari Prawej
Published: April 2024

Mangifera indica (Anacardiaceae family) is renowned for its diverse pharmacological properties, encompassing antidiabetic, antioxidant, and anti-inflammatory effects. The present study delves into the insulin-releasing and glucose-lowering potential of the ethanolic extract of Mangifera indica (EEMI) leaves in streptozotocin-induced type 2 diabetic (STZ-T2D) rats, concurrently investigating its phytoconstituents. EEMI’s effects on insulin secretion were measured using BRIN BD11 β-cells and isolated mouse islets. Its enzymatic inhibitory properties on carbohydrate digestion, and absorption, and free radicals were investigated using in vitro methods. In vivo parameters including the lipid profile and liver glycogen content were assessed in STZ-T2D rats. EEMI exhibited a dose-dependent increase in insulin secretion from clonal pancreatic BRIN BD11 β-cells and isolated mouse islets. EEMI inhibited starch digestion, glucose diffusion over time, and DPPH activity in vitro. In acute in vivo studies, EEMI improved food intake and oral glucose tolerance. Moreover, following 28 days of treatment with EEMI, a remarkable amelioration in body weight, fasting blood glucose, plasma insulin, liver glycogen content, total cholesterol, triglyceride, LDL, VLDL, and HDL levels was observed. Further phytochemical analysis with EEMI identified the presence of alkaloids, tannins, saponins, steroids, and flavonoids. The synergistic effects of EEMI, potentially attributable to naturally occurring phytoconstituents, hold promise for the development of enriched antidiabetic therapies, offering a promising avenue for the management of type 2 diabetes.
All about Delay-Tolerant Networking (DTN) Contributions to Future Internet
Authors: Koukis Georgios , Safouri Konstantina , Tsaoussidis Vassilis
Published: April 2024

Although several years have passed since its first introduction, the significance of Delay-Tolerant Networking (DTN) remains evident, particularly in challenging environments where traditional networks face operational limitations such as disrupted communication or high latency. This survey paper aims to explore the diverse array of applications where DTN technologies have proven successful, with a focus on emerging and novel application paradigms. In particular, we focus on the contributions of DTN in the Future Internet, including its contribution to space applications, smart cities and the Internet of Things, but also to underwater communications. We also discuss its potential to be used jointly with information-centric networks to change the internet communication paradigm in the future.
The Relationship between Mean Length at Maturity and Maximum Length in Coral Reef Fish
Authors: Chu Elaine , Pauly Daniel
Published: April 2024

This article proposes a mechanism that triggers first maturation and spawning in coral reef (bony) fish, which allows for predicting their length at first maturity. Thus, mean lengths at first maturity (Lm) and the corresponding maximum lengths (Lmax) in 207 populations of 131 species of coral reef fish were assembled and used to test the hypotheses that (a) there is, in coral reef fish, a single value of a size-related parameter acting as a trigger for their maturation and eventual spawning, and (b) that this single value is statistically the same as that published previously for other bony fish. The results, based on the assembled Lm and Lmax data and on estimates of the parameter D, which link the length of fish with the relative surface of their gills, covered 44 families and Lmax values ranging from 1.8 to 181.6 cm and confirmed that the threshold in (a) exists. Also, we assessed (in b) that this threshold value, i.e., LmaxD/LmD = 1.35 (±0.02), is not statistically different from similar estimates for other groups of teleosts, notably semelparous salmonids, cichlids, sturgeons and Chinese and Turkish freshwater and marine fish. One implication is that given ocean warming and deoxygenation, coral reef fish will not only be smaller than they currently are, but also mature and spawn at smaller sizes, and thus produce fewer, smaller eggs.
Simulation-Based Investigation of On-Demand Vehicle Deployment for Night Bus Routes Using the Monte Carlo Method
Authors: Wang Shen , Weber Thomas , Schramm Dieter , Berns Thorben
Published: April 2024

Public transportation systems, including trams and buses, play a crucial role in urban traffic. However, these traditional modes of transport have some well-known drawbacks, such as long distances between stops, lengthy waiting times, and a lack of privacy. In response to these challenges, an innovative mobility concept called “FLAIT-train” offers potential solutions. The FLAIT-train operates on regular roads and aims to provide DOOR-2-DOOR transport, addressing the issues associated with fixed stops and offering increased accessibility and convenience. In its initial phase, the FLAIT-train operates on exclusive lanes, but it is designed to integrate with other traffic eventually. The vehicle technology of FLAIT-trains closely resembles that of battery electric autonomous vehicles. To assess whether FLAIT-trains can be used as a suitable alternative to conventional public transportation systems, this paper employs traffic simulations that consider key performance indicators, including the average waiting time per passenger, maximum waiting time of a single passenger, average in-vehicle time per passenger, and average occupancy rate of the vehicles. Using SUMO software (“Simulation of Urban Mobility”, version 1.12.0), a night bus service scenario is meticulously designed and generated. Within this scenario, both FLAIT-trains and conventional buses are simulated under identical conditions and based on statistical data.
Increased Serum Apelin Levels in Patients with Inflammatory Bowel Disease
Authors: Mantaka Aikaterini , Kalyvianaki Konstantina , Kastritsi Olga , Kampa Marilena , Koutroubakis Ioannis E.
Published: April 2024

Apelin has been implicated in the pathogenesis of several chronic inflammatory diseases through mechanisms related to endothelial cells dysfunction. There is evidence of increased apelin levels in mesenteric adipose tissue and colonic epithelium in patients with inflammatory bowel disease (IBD), but their significance remains unclear. We aimed to measure serum apelin (SA) levels in patients with IBD and to evaluate an association with disease characteristics. SA levels of 104 IBD patients and age and sex matched healthy controls (HCs) in a 1:1 ratio were compared. SA-13 levels were measured using an enzyme-linked immunosorbent assay (ELISA). Mean SA levels were increased in IBD patients compared to HCs (1996.29 ± 1592.96 pg/mL vs. 1552.99 ± 809.64 pg/mL, p = 0.012). Both patients without and with cardiovascular disease (CVD) had increased SA levels (2076.44 ± 1714.74 pg/mL vs. 1525.75 ± 818.74 pg/mL, p = 0.011 and 1743.01 ± 1116.26 pg/mL vs. 1283.92 ± 726.85 pg/mL, p = 0.035, respectively). An inverse association between mean SA levels and a history of musculoskeletal extraintestinal manifestations in the subgroup of IBD patients without CVD was found (p = 0.043). The present study—the first to evaluate SA levels in patients with IBD—showed that IBD patients have higher levels of SA compared to HCs. The potential role of SA in IBD merits further investigation in larger studies.
Interventional Treatment of Malignant Biliary Obstruction: Is It Time to Change the Paradigm?
Authors: Hristov Bozhidar , Doykov Daniel , Andonov Vladimir , Doykov Mladen , Kraev Krasimir , Uchikov Petar , Dimov Rosen , Kostov Gancho , Valova Siyana , Doykova Katya , Chakarov Dzhevdet , Sandeva Milena
Published: April 2024

Introduction. Biliary obstruction is a common manifestation of biliopancreatic malignancies, and its relief is an essential part of the treatment algorithm. Currently, there are three techniques to manage malignant biliary obstruction—endoscopic retrograde cholangiopancreatography (ERCP), percutaneous transhepatic biliary drainage (PTBD), and endoscopic ultrasound-guided biliary drainage (EUS-BD). ERCP has been adopted as a first-line treatment modality but EUS-BD is gradually emerging as a viable alternative. The aim of the current article is to assess the clinical outcomes of the three nonsurgical biliary drainage procedures. Materials and methods. A total of 102 consecutive patients with unresectable biliopancreatic malignancy inducing biliary obstruction and subjected to palliative treatment by means of ERCP, EUS-BD, or PTBD were retrospectively included in the study. Results. No difference in clinical and technical success of the procedures was found: ERCP—97.2% technical; 88.9% clinical; PTBD—94.4% technical, 72.2% clinical; EUS-BD—90% technical; 83.3% clinical. Adverse events (AEs) and reinterventions were significantly more common in PTBD (38.9% and 52.8%) and ERCP (27.9% and 25%) compared to EUS-BD (10% and 3.3%). Total duration of hospital stay and number of hospitalizations were lower in the EUS-BD compared to PTBD and ERCP groups. Conclusions. In the presence of adequate expertise, EUS-BD may be superior to PTBD and ERCP in achieving and sustaining biliary drainage in the setting of unresectable malignancy.
Identification of Laccase Genes in Grapevine and Their Roles in Response to Botrytis cinerea
Authors: Wan Ran , Yang Zhenfeng , Liu Jun , Zhang Mengxi , Jiao Jian , Wang Miaomiao , Zhang Kunxi , Hao Pengbo , Liu Yu , Bai Tuanhui , Song Chunhui , Song Shangwei , Shi Jiangli , Zheng Xianbo
Published: April 2024

Laccases are the key enzymes responsible for plant lignin biosynthesis and responses to environment stress. However, the roles of LAC genes in plant disease resistance are still largely unknown, especially in grapevine, one of the most important horticultural crops in the world. Its quality and yield are very vulnerable to gray mold disease caused by Botrytis cinerea. In total, 30 VvLAC genes were identified and found to be unevenly distributed on seven chromosomes; they were classified into seven groups based on phylogenetic analysis according to the criteria applied in Arabidopsis thaliana. Collinearity and synteny analyses identified some orthologous gene pairs in Vitis vinifera and a few paralogous gene pairs among grape and peach. The VvLAC gene family has diverse gene structures and a highly conserved motif composition. The prominent presence of the MYB cis-elements in each VvLAC promoter highlighted MYB transcriptional factors as the main regulators of VvLAC genes. Furthermore, twenty-five VvLAC genes with functional redundancy are probably implicated in grape lignin biosynthesis. The expression patterns of the LAC genes in grape leaves of Chinese wild V. amurensis ‘Shuangyou’ (SY), a germplasm highly resistant to B. cinerea, were investigated through transcriptomic data and qRT-PCR verification. Combined with the phylogenetic analysis, with AtLACs participating in lignin metabolism, and the cis-element analysis, VaLAC14, VaLAC19, VaLAC24 and VaLAC30 were identified as key candidate genes for lignin biosynthesis in the grape response to B. cinerea. This study supplies a comprehensive understanding of the classification, evolution, structure and responses of the grape LAC genes against B. cinerea. It also provides valuable genetic resources for functional characterization towards enhancing grapevine disease resistance.
Special Issue: ‘Preharvest and Postharvest Factors Improving Horticultural Crops Quality and Shelf-Life’
Authors: Gong Di , Bi Yang , Li Yongcai
Published: April 2024

Although fresh horticultural crops are rich in nutrients and various biologically active chemicals, they are also perishable [...]
An Investigation into the Prevalence of Methamphetamine Related Enquiries to Local Government Environmental Health Officers
Authors: Kuhn Emma J. , Ross Kirstin E. , Walker G. Stewart , Wright Jackie , Whiley Harriet
Published: April 2024

Methamphetamine contamination of residential properties remains a serious public health concern for members of the public. External stakeholders including Environmental Health Officers (EHOs) and testing and remediation technicians are engaged on investigating whether contamination has occurred from manufacturing or smoking processes. More specifically, local council EHOs are responsible for managing clandestine drug laboratories when notified by police and also for responding to public enquiries. However, the full scope of these contaminated properties is not seen by any single stakeholder, making it very challenging to quantify these situations. To evaluate the prevalence of methamphetamine related enquiries from the general public to EHOs, this study surveyed and interviewed officers from around Australia. It was found that public enquiries were infrequent with only 6% of respondents having received enquiries in the last month, which indicates that people are seeking information from other sources. Interestingly, there were case study scenarios that also mentioned issues with awareness and the flow of information. Concerns regarding difficult cases, police notifications, and site visits were also highlighted. The results of this study provide a benchmark of how methamphetamine related cases are managed and highlight the need for trustworthy information that is available to EHOs, governments, industry members, and the public in a unified location.
Teen Perspectives on Suicides and Deaths in an Affluent Community: Perfectionism, Protection, and Exclusion
Authors: Peterson Abigail , Smith-Morris Carolyn
Published: April 2024

Clusters of youth suicide and death are tragic for communities and present long-term consequences for the surviving youths. Despite an awareness of community-based patterns in youth suicide, our understanding of the social and community factors behind these events remains poor. While links between poverty and suicide have been well documented, wealthy communities are rarely targeted in suicide research. In response to this gap, we conducted ethnographic research in a wealthy U.S. town that, over a recent 10-year period, witnessed at least four youth suicides and seven more youth accidental deaths. Our interviews (n = 30) explored community values and stressors, interpersonal relationships, and high school experiences on participant perceptions of community deaths. Youth participants characterize their affluent community as having (1) perfectionist standards; (2) permissive and sometimes absent parents; (3) socially competitive and superficial relationships; and (4) a “bubble” that is protective but also exclusionary. Our qualitative findings reveal network influence in teen suicides and accidental deaths in a wealthy community. Greater attention paid to the negative effects of subcultural values and stressors in affluent communities is warranted. Further, our work promotes the value of ethnographic, community-based methodologies for suicidology and treatment.
Patterns and Predictors of HIV Comorbidity among Adolescents and Young Adults in South Africa
Authors: van Wyk Brian , Roomaney Rifqah Abeeda
Published: April 2024

Adolescents and young adults (AYA) are identified as a high-risk group for HIV acquisition. However, health services are generally not sensitive to the needs of this priority population. In addition, multimorbidity (having more than one disease in an individual) is not well studied among AYA, as it is typically associated with older individuals. This paper reports on commonly co-occurring disease conditions and disease patterns in AYA, aged 15–24 years, who took part in the 2016 South African Demographic and Health Survey. Chi-squared tests and logistic regression were used to examine the weighted prevalence of disease among those with/without HIV, and the risk factors associated with HIV. Latent class analysis (LCA) was conducted to identify common co-occurring diseases. Of the 1787 individuals included in our analysis, the weighted prevalence of HIV was 8.7%. Hypertension (30.5%), anaemia (35.8%) and diabetes (2.0%) were more prevalent among those with HIV. HIV and anaemia, hypertension and anaemia, and HIV and hypertension comprise the largest disease burden of co-occurring diseases. Co-morbidity was high among those with HIV, emphasizing the need for integrated care of HIV and non-communicable diseases.
Assessing the Impact of Quarrying as an Environmental Ethic Crisis: A Case Study of Limestone Mining in a Rural Community
Authors: Kafu-Quvane Babalwa , Mlaba Sanelisiwe
Published: April 2024

In this study, we investigated the impact of quarrying as an environmental ethical crisis. The need for the study arose when we realised the deteriorating effect of the quality of life in our community, which is located next to a limestone quarry. To obtain a deeper understanding of the adverse impact on the environment and the quality of life of the people living in the community around the quarry, we explored the workers from the community, and the members of the community-based organisation’s (CBO) experiences. We employed a qualitative method research approach, using a single case study design. We adopted a utilitarian perspective and Pinchot’s conservation as ethical systems that determine morality based on the greatest good for the greatest number. Both provide a framework for analysing environmental problems and ethical crises associated with limestone quarrying. We generated data using face-to-face interviews and focus group discussions. We present and discuss data through the following themes: analysis of the social and cultural impacts on local communities and indigenous people, assessment of the ecological consequences on biodiversity and habitat destruction, and examination of the effects on water resources, air quality, and soil erosion. The results show that the negative effects of the quarry on the environment have always worried the local people. The company’s disrespect for the community and ignorance of the laws governing quarry activities is the root of the ethical dilemma. The detrimental effects that the operations have on human health and safety as well as the environment is the other ethical dilemma, which includes, land degradation, vibrations, air, and water pollution.
Maternal Vitamin D Deficiency Impairs the Development of β Cells in Offspring Rats in a Sex-Dependent Manner
Authors: Schavinski Aline Z. , Reis Natany G. , Morgan Henrique J. N. , Assis Ana Paula , Moro Matheus L. , Valentim Rafael R. , Seni-Silva Ana Carolina , Ramos Ester S. , Kettelhut Isis C. , Navegantes Luiz C. C.
Published: April 2024

Recent studies have shown that maternal vitamin D deficiency (VDD) causes long-term metabolic changes in offspring. However, little is known about the impact of maternal VDD on offspring endocrine pancreas development and insulin secretion in the adult life of male and female animals. Female rats (Wistar Hannover) were fed either control (1000 IU Vitamin D3/kg), VDD (0 IU Vitamin D3/kg), or a Ca2+-enriched VDD diet (0 IU Vitamin D3/kg + Ca2+ and P/kg) for 6 weeks and during gestation and lactation. At weaning, VDD status was confirmed based on low serum calcidiol levels in dams and pups. Next, male and female offspring were randomly separated and fed a standard diet for up to 90 days. At this age, serum calcidiol levels were restored to normal levels in all groups, but serum insulin levels were decreased in VDD males without affecting glucagon levels, glycemia, or glucose tolerance. Islets isolated from VDD males showed lower insulin secretion in response to different glucose concentrations, but this effect was not observed in VDD females. Furthermore, VDD males, but not females, showed a smaller total pancreatic islet area and lower β cell mass, an effect that was accompanied by reduced gene expression of Ins1, Ins2, Pdx1, and SLC2A2. The decrease in Pdx1 expression was not related to the methylation profile of the promoter region of this gene. Most of these effects were observed in the male VDD+Ca2+ group, indicating that the effects were not due to alterations in Ca2+ metabolism. These data show that maternal VDD selectively impairs the morphology and function of β cells in adult male offspring rats and that female offspring are fully protected from these deleterious effects.
Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells
Authors: Dzięgelewska-Sokołowska Żaneta , Majewska Alicja , Szopa Iwona , Gajewska Małgorzata
Published: April 2024

Adipose tissue is an active endocrine gland, synthesizing and secreting multiple signaling molecules termed adipokines. Following the detection of adipokines and their receptors in the mammary tissue of various species, it is indicated that adipokines play a role in the development of the mammary gland. The aim of the present study was to determine the concentration-dependent influence of three adipokines, leptin, adiponectin, and chemerin, on the viability, apoptosis, and secretory activity of BME-UV1 bovine mammary epithelial cells. The study confirmed that BME-UV1 cells contain the leptin receptor (Ob-R) protein, and express transcripts of adiponectin (ADIPOR1 and ADIPOR2) and chemerin (CMLKR1 and GPR1) receptors. Regardless of the administered dose, none of the three tested adipokines had an effect on the viability of BME-UV1 cells, and the number of apoptotic cells remained unchanged. However, chemerin (100 ng/mL) stimulated BME-UV1 cells to synthesize and secrete αS1-casein, the major protein component of milk. These results indicate that chemerin may be a potent regulator of the bovine mammary epithelial cells’ functional differentiation, contributing, along with the major systemic hormones and local growth factors, to the development of the bovine mammary gland.
Novel Tetracyclic Azaphenothiazines with the Quinoline Ring as New Anticancer and Antibacterial Derivatives of Chlorpromazine
Authors: Jeleń Małgorzata , Otto-Ślusarczyk Dagmara , Morak-Młodawska Beata , Struga Marta
Published: April 2024

Phenothiazine derivatives are widely studied in various fields such as biology, chemistry, and medicine research because of their pharmaceutical effects. The first compound used successfully in the treatment of psychosis was a phenthiazine derivative, chlorpromazine. Apart from its activity in neurons, chlorpromazine has also been reported to display anticancer and antibacterial properties. In this study, we present the synthesis and research on the activity of A549, MDA, MiaPaCa, PC3, and HCT116 cancer cell lines and of S. aureus, S. epidermidis, E. coli, and P. aeruginosa bacterial strains against a series of new tetracyclic chlorpromazine analogues containing a quinoline scaffold in their structure instead of the benzene ring and various substituents at the thiazine nitrogen. The structure of these novel molecules has been determined by 1H NMR, 13C NMR, and HRMS spectral techniques. The seven most active of the twenty-four new chlorpromazine analogues tested were selected to study the mechanism of cytotoxic action. Their ability to induce apoptosis or necrosis in cancer cells was assessed by flow cytometry analysis. The results obtained confirmed the proapoptotic activity of selected compounds, especially in terms of inducing late apoptosis or necrosis in cancer cell lines A549, MiaPaCa-2, and HCT-116. Furthermore, studies on the induction of cell cycle arrest suggest that the new chlorpromazine analogues exert antiproliferative effects by inducing cell cycle arrest in the S phase and, consequently, apoptosis.
Comparative Screening of the Liver Gene Expression Profiles from Type 1 and Type 2 Diabetes Rat Models
Authors: Guerra-Ávila Paloma Lucía , Guzmán Tereso J. , Vargas-Guerrero Belinda , Domínguez-Rosales José Alfredo , Cervantes-Garduño Alejandra Beatriz , Salazar-Montes Adriana María , Sánchez-Orozco Laura Verónica , Gurrola-Díaz Carmen Magdalena
Published: April 2024

Experimental animal models of diabetes can be useful for identifying novel targets related to disease, for understanding its physiopathology, and for evaluating emerging antidiabetic treatments. This study aimed to characterize two rat diabetes models: HFD + STZ, a high-fat diet (60% fat) combined with streptozotocin administration (STZ, 35 mg/kg BW), and a model with a single STZ dose (65 mg/kg BW) in comparison with healthy rats. HFD + STZ- induced animals demonstrated a stable hyperglycemia range (350–450 mg/dL), whereas in the STZ-induced rats, we found glucose concentration values with a greater dispersion, ranging from 270 to 510 mg/dL. Moreover, in the HFD + STZ group, the AUC value of the insulin tolerance test (ITT) was found to be remarkably augmented by 6.2-fold higher than in healthy animals (33,687.0 ± 1705.7 mg/dL/min vs. 5469.0 ± 267.6, respectively), indicating insulin resistance (IR). In contrast, a more moderate AUC value was observed in the STZ group (19,059.0 ± 3037.4 mg/dL/min) resulting in a value 2.5-fold higher than the average exhibited by the control group. After microarray experiments on liver tissue from all animals, we analyzed genes exhibiting a fold change value in gene expression <−2 or >2 (p-value <0.05). We found 27,686 differentially expressed genes (DEG), identified the top 10 DEGs and detected 849 coding genes that exhibited opposite expression patterns between both diabetes models (491 upregulated genes in the STZ model and 358 upregulated genes in HFD + STZ animals). Finally, we performed an enrichment analysis of the 849 selected genes. Whereas in the STZ model we found cellular pathways related to lipid biosynthesis and metabolism, in the HFD + STZ model we identified pathways related to immunometabolism. Some phenotypic differences observed in the models could be explained by transcriptomic results; however, further studies are needed to corroborate these findings. Our data confirm that the STZ and the HFD + STZ models are reliable experimental models for human T1D and T2D, respectively. These results also provide insight into alterations in the expression of specific liver genes and could be utilized in future studies focusing on diabetes complications associated with impaired liver function.