bluuebunny/crossref_metadata_2025 huggingface dataset:
Li-Distribution in Compounds of the Li2O-MgO-Al2O3-SiO2-CaO System—A First Survey
Authors: Schirmer Thomas , Qiu Hao , Li Haojie , Goldmann Daniel , Fischlschweiger Michael
Published: December 2020

The recovery of critical elements in recycling processes of complex high-tech products is often limited when applying only mechanical separation methods. A possible route is the pyrometallurgical processing that allows transferring of important critical elements into an alloy melt. Chemical rather ignoble elements will report in slag or dust. Valuable ignoble elements such as lithium should be recovered out of that material stream. A novel approach to accomplish this is enrichment in engineered artificial minerals (EnAM). An application with a high potential for resource efficient solutions is the pyrometallurgical processing of Li ion batteries. Starting from comparatively simple slag compositions such as the Li-Al-Si-Ca-O system, the next level of complexity is reached when adding Mg, derived from slag builders or other sources. Every additional component will change the distribution of Li between the compounds generated in the slag. Investigations with powder X-Ray diffraction (PXRD) and electron probe microanalysis (EPMA) of solidified melt of the five-compound system Li2O-MgO-Al2O3- SiO2-CaO reveal that Li can occur in various compounds from beginning to the end of the crystallization. Among these compounds are Li1−x(Al1−xSix)O2, Li1−xMgy(Al)(Al3/2y+xSi2−x−3/2y)O6, solid solutions of Mg1−(3/2y)Al2+yO4/LiAl5O8 and Ca-alumosilicate (melilite). There are indications of segregation processes of Al-rich and Si(Ca)-rich melts. The experimental results were compared with solidification curves via thermodynamic calculations of the systems MgO-Al2O3 and Li2O-SiO2-Al2O3.
Influence of Laser Surface Texturing Sequence on Fatigue Properties of Coated Cold Work Tool Steel
Authors: Sedlaček Marko , Guštin Agnieszka Zuzanna , Žužek Borut
Published: December 2020

The aim of this research was to investigate the influence of laser surface texturing sequence on the fatigue properties of cold-work tool steel. For this reason, polished hourglass-type test specimens made from cold-work tool steel (K890) were surface textured using laser texturing. Surface textures were introduced before and after hard coating deposition (TiAlN) with the aim to investigate the sequence of surface-texturing process. It was found that coating deposition prolongs the fatigue life. The fatigue life behaviour can be influenced also by the sequence of surface texturing. In the case when laser texturing is done after coating deposition, it suffers in fatigue life properties. From a lower magnification, a fractured surface looks like a quasi-ductile fracture, but a closer look reveals that there is very little plastic deformation and some small flat regions can be seen with clear evidence of a brittle fracture mechanism with cleavage. Due to low fracture toughness of investigated steel, no fatigue striations of crack growth steps were found on the fractured surfaces.
Modeling of the Effect of the Building Strategy on the Thermomechanical Response of Ti-6Al-4V Rectangular Parts Manufactured by Laser Directed Energy Deposition
Authors: Lu Xufei , Cervera Miguel , Chiumenti Michele , Li Junjie , Ji Xianglin , Zhang Guohao , Lin Xin
Published: December 2020

Part warpage and residual stress are two of the main challenges for metal additive manufacturing (AM) as they result in lower geometric precision and poor mechanical properties of the products. This work investigates the effect of the building strategy on the heat transfer process and the evolution of the thermally induced mechanical variables in laser directed energy deposition (L-DED) in order to minimize residual stresses and deformations. A 3D finite element (FE) thermo-mechanical model is firstly calibrated through in-situ experiments of rectangular workpieces fabricated by L-DED technology, and, secondly, the coupled thermo-mechanical responses for different process parameters and scanning patterns are discussed in detail. On the calibration stage, the remarkable agreement is achieved between predicted results and experimental data. Regarding the modeling stage, the numerical results indicate that minimization of the part warpage is achieved by reducing the back speed and shortening the scanning lines during the building process. Both residual stress and deformation can be further reduced if preheating the baseplate is added before L-DED.
Sustainability Assessment, Investigations, and Modelling of Slot Milling Characteristics in Eco-Benign Machining of Hardened Steel
Authors: Markopoulos Angelos P. , Karkalos Nikolaos E. , Mia Mozammel , Pimenov Danil Yurievich , Gupta Munish Kumar , Hegab Hussein , Khanna Navneet , Aizebeoje Balogun Vincent , Sharma Shubham
Published: December 2020

The hardened tool steel AISI O1 has increased strength, hardness, and wear resistance, which affects the complexity of the machining process. AISI O1 has also been classified as difficult to cut material hence optimum cutting parameters are required for the sustainable machining of the alloy. In this work, the effect of feed peer tooth (fz), cutting speed (vc), cutting of depth (ap) on surface roughness (Ra, Rt), cutting force (Fx, Fy), cutting power (Pc), machining cost (Ci), and carbon dioxide (Ene) were investigated during the slot milling process of AISI O1 hardened steel. A regression analysis was carried out on the obtained experimental results and the induction of nonlinear mathematical equations of surface roughness, cutting force, cutting power, and machining cost with a high coefficient of determination (R2 = 90.62–98.74%) were deduced. A sustainability assessment model is obtained for optimal and stable levels of design variables when slot milling AISI O1 tool steel. Stable indicators to ensure personal health and safety of operation, P1 values were set to “1” at a cutting speed of 20 m/min or 43.3 m/min and “2” at a cutting speed of 66.7 m/min or 90 m/min. It is revealed that for eco-benign machining of AISI O1, the optimum parameters of 0.01 mm/tooth, 20 m/min, and 0.1 mm should be adopted for feed rate, cutting speed, and depth of cut respectively.
Phytochemical Profile and Antioxidant Activity of Aerial and Underground Parts of Salvia bulleyana Diels. Plants
Authors: Grzegorczyk-Karolak Izabela , Krzemińska Marta , Kiss Anna K. , Olszewska Monika A. , Owczarek Aleksandra
Published: December 2020

Plants have been used for medical purposes since ancient times. However, a detailed analysis of their biological properties and their associated active compounds is needed to justify their therapeutic use in modern medicine. The aim of the study was to identify and quantify the phenolics present in hydromethanolic extracts of the roots and shoots of the Chinese Salvia species, Salvia bulleyana. The qualitative and quantitative analyses were carried out by ultrahigh-performance liquid chromatography with electrospray ionization mass spectrometry detection (UHPLC-PDA-ESI-MS), and high-performance liquid chromatography with photodiode array (HPLC-PDA) detection. The extracts of S. bulleyana were also screened for their antioxidant activity using ferric ion (Fe3+) reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) cation (ABTS), superoxide radical anion (O2•–), and inhibition of lipid peroxidation assays. The S. bulleyana extracts were found to contain 38 substances, of which 36 were phenols, with a total level of 14.4 mg/g DW (dry weight) in shoots, and 23.1 mg/g DW in roots. Twenty-eight phenols were polyphenolic acids or their derivatives, the most abundant in shoots being rosmarinic acid, and in roots, salvianolic acid K followed by rosmarinic acid. The other major phenolic acids were caffeic acid, caffeoyl-threonic acids, isomers of lithospermic acid, salvianolic acid F, salvianolic acid B, and yunnaneic acid E. In addition to polyphenolic acids, nine flavonoids were detected in the shoot extract. While both extracts showed significant antioxidant activity, the shoot extract, containing both polyphenolic acids and flavonoids, demonstrated a slightly greater antioxidant potential in some of the anti-radical tests than the roots. However, the root extract proved to be slightly more effective in the lipid peroxidation inhibition test. Thus, S. bulleyana was demonstrated as a promising source of antioxidants, and worthy of further more detailed studies.
Vitamin D Metabolism and Its Role in Mineral and Bone Disorders in Chronic Kidney Disease in Humans, Dogs and Cats
Authors: Chacar Fernanda C. , Kogika Márcia M. , Zafalon Rafael V. A. , Brunetto Marcio A.
Published: December 2020

Some differences regarding Vitamin D metabolism are described in dogs and cats in comparison with humans, which may be explained by an evolutionary drive among these species. Similarly, vitamin D is one of the most important regulators of mineral metabolism in dogs and cats, as well as in humans. Mineral metabolism is intrinsically related to bone metabolism, thus disturbances in vitamin D have been implicated in the development of chronic kidney disease mineral and bone disorders (CKD-MBD) in people, in addition to dogs and cats. Vitamin D deficiency may be associated with Renal Secondary Hyperparathyroidism (RSHPT), which is the most common mineral disorder in later stages of CKD in dogs and cats. Herein, we review the peculiarities of vitamin D metabolism in these species in comparison with humans, and the role of vitamin D disturbances in the development of CKD-MBD among dogs, cats, and people. Comparative studies may offer some evidence to help further research about vitamin D metabolism and bone disorders in CKD.
Genotyping of Francisella tularensis subsp. holarctica from Hares in Germany
Authors: Linde Jörg , Homeier-Bachmann Timo , Dangel Alexandra , Riehm Julia M. , Sundell David , Öhrman Caroline , Forsman Mats , Tomaso Herbert
Published: December 2020

Francisella tularensis is the causative agent of the zoonotic disease tularemia. In Germany, most human infections are caused by contact with infected hares. The aim of this study was to characterize Francisella tularensis subsp. holarctica strains isolated from hares in Germany and to develop bioinformatics tools to analyze their genetic relatedness. In total, 257 German isolates—obtained mainly from hares (n = 233), other vertebrate animals, and ticks, but also from humans (n = 3)—were analyzed within this study. Publically available sequence data from 49 isolates were used to put our isolates into an epidemiological context and to compare isolates from natural foci and humans. Whole-genome sequences were analyzed using core-genome Multi-Locus-Sequence-Typing, canonical Single Nucleotide Polymorphism (SNP) typing and whole-genome SNP typing. An overall conformity of genotype clustering between the typing methods was found, albeit with a lower resolution for canonical single SNP typing. The subclade distribution, both on local and national levels, among strains from humans and hares was similar, suggesting circulation of the same genotypes both in animals and humans. Whilst close to identical isolates of the same subclade were found distributed over large areas, small geographical foci often harbored members of different subclades. In conclusion, although genomic high-resolution typing was shown to be robust, reproducible and allowed the identification of highly closely related strains, genetic profiling alone is not always conclusive for epidemiological linkage of F. tularensis strains.
Untapped Potentials of Endophytic Fungi: A Review of Novel Bioactive Compounds with Biological Applications
Authors: Manganyi Madira Coutlyne , Ateba Collins Njie
Published: December 2020

Over the last century, endophytic fungi have gained tremendous attention due to their ability to produce novel bioactive compounds exhibiting varied biological properties and are, therefore, utilized for medicinal, pharmaceutical, and agricultural applications. Endophytic fungi reside within the plant tissues without showing any disease symptoms, thus supporting the physiological and ecological attributes of the host plant. Ground breaking lead compounds, such as paclitaxel and penicillin, produced by endophytic fungi have paved the way for exploring novel bioactive compounds for commercial usage. Despite this, limited research has been conducted in this valuable and unique niche area. These bioactive compounds belong to various structural groups, including alkaloids, peptides, steroids, terpenoids, phenols, quinones, phenols, and flavonoids. The current review focuses on the significance of endophytic fungi in producing novel bioactive compounds possessing a variety of biological properties that include antibacterial, antiviral, antifungal, antiprotozoal, antiparasitic, antioxidant, immunosuppressant, and anticancer functions. Taking into consideration the portal of this publication, special emphasis is placed on the antimicrobial and antiviral activities of metabolites produced by endophytes against human pathogens. It also highlights the importance of utilization of these compounds as potential treatment agents for serious life-threatening infectious diseases. This is supported by the fact that several findings have indicated that these bioactive compounds may significantly contribute towards the fight against resistant human and plant pathogens, thus motivating the need enhance the search for new, more efficacious and cost-effective antimicrobial drugs.
Improved Exopolymer Production by Chromohalobacter canadensis Cultures for Its Potential Cosmeceutical Applications
Authors: Radchenkova Nadja , Hasköylü Merve Erginer , Vassilev Spasen , Yıldız Songül Yaşar , Boyadzhieva Ivanka , Oner Ebru Toksoy , Kambourova Margarita
Published: December 2020

Several exopolymers with different chemical composition and correspondingly variety in their physico-chemical properties from halophilic microorganisms have still been described, however, with a low production yield. Chromohalobacter canadensis 28 isolated from Pomorie saltern synthesized an unusual exopolymer (EP) containing 72% γ-polyglutamic acid (PGA), an essential cosmeceutical additive. Current work suggests a novel approach for effective EP synthesis by C. canadensis 28 using continuous cultures. Highest production was observed at low dilution rates reaching a level of 2.1 mg/mL at D = 0.035, similar to those in batch cultures (2.34 mg/mL), however avoiding all disadvantages of discontinuous fermentation processes. At steady state, the total quantities of the synthesized EP after 48 h cultivation for the given equipment volume in D = 0.035 h−1 and D = 0.075 h−1 were 8.67 and 12 g, correspondingly, while it was 2.9 g for batch culture. Process parameters did not change after a ten-day run at D = 0.35 h−1. A degree of purity of EP fraction received from continuous cultures was significantly increased up to 93–96%. A lack of cytotoxicity and high cell viability were observed for human dermal fibroblast cells after 24 h incubation with crude EP from C. canadensis 28 and purified PGA fraction that could suggest its high potential for cosmetic applications.
Preservation, Characterization and Exploitation of Microbial Biodiversity of Agri-Food and Environmental Interest
Authors: Mannazzu Ilaria , Budroni Marilena , Zara Giacomo , Zara Severino
Published: December 2020

This Special Issue collects original contributions in the form of review or research articles, dealing with different aspects of the preservation, characterization and exploitation of the biodiversity of bacteria, yeast, algae and filamentous fungi of different origins [...]
Impacts of Wet Market Modernization Levels and Hygiene Practices on the Microbiome and Microbial Safety of Wooden Cutting Boards in Hong Kong
Authors: Ngan Wing Yui , Rao Subramanya , Chan Long Chung , Sekoai Patrick T. , Pu Yang , Yao Yuan , Fung Aster Hei Yiu , Habimana Olivier
Published: December 2020

Accessing food through wet markets is a common global daily occurrence, where fresh meat can be purchased to support an urbanizing world population. Similar to the wet markets in many other metropolitan cities in Asia, Hong Kong wet markets vary and are characterized by differing hygiene routines and access to essential modern technologies. The lack of risk assessments of food contact surfaces in these markets has led to substantial gaps in food safety knowledge and information that could help improve and maintain public health. Microbial profiling analyses were conducted on cutting boards that had been used to process pork, poultry, and seafood at 11 different wet markets. The markets differed in hygiene protocols and access to modern facilities. Irrespective of whether wet markets have access of modern infrastructure, the hygiene practices were largely found to be inefficient based on the prevalence of bacterial species typically associated with foodborne pathogens such as Campylobacter fetus, Clostridium perfringens, Staphylococcus aureus, and Vibrio parahaemolyticus; indicator organisms such as Escherichia coli; as well as nonfoodborne pathogenic bacterial species potentially associated with nosocomial infections, such as Klebsiella pneumoniae and Enterobacter cloacae. Other Vibrio species, V. parahaemolyticus and V. vulnificus, typically associated with contaminated raw or undercooked seafood with the potential to cause illness in humans, were also found on wooden cutting boards. This study indicated that the hygienic practices used in Hong Kong wet markets are not sufficient for preventing the establishment of spoilage or pathogenic organisms. This study serves as a basis to review current hygiene practices in wet markets and provides a framework to reassess existing safety protocols.
Magnesium Leachability of Mg-Silicate Peridotites: The Effect on Magnesite Yield of a Mineral Carbonation Process
Authors: Rashid Muhammad Imran , Benhelal Emad , Farhang Faezeh , Stockenhuber Michael , Kennedy Eric M.
Published: December 2020

The aim of this study was to increase feedstock availability for mineral carbonation. Acid dissolution and carbonic acid dissolution approaches were used to achieve higher Mg extractions from peridotites. Acid dissolution studies of raw dunite, heat-activated dunite, heat-transformed dunite, and twin sister dunite have not been reported in the literature. Heat-activated dunite is more reactive as compared to heat-transformed dunite, raw dunite, and twin sister dunite. The fraction of magnesium extracted from heat-activated dunite was 57% as compared to 18% from heat-transformed dunite, 14% from raw dunite, and 11% from twin sister dunite. Similarly, silicon and iron extractions were higher for heat-activated dunite compared to that of heat-transformed dunite, raw dunite, and twin sister dunite. Materials rich in forsterite (twin sister dunite and heat-transformed dunite) showed preferential Mg release and exhibited incongruent dissolution similar to that of forsterite. Heat-activated dunite (amorphous magnesium silicate rich) on the other hand behaved differently and showed congruent dissolution. Olivine did not dissolve under carbonic acid dissolution (with concurrent grinding) and acidic conditions. Under carbonic acid dissolution with concurrent grinding conditions, olivine was partially converted into nanometer sized particles (d10 = 0.08 µm) but still provided 16% Mg extraction during 4 h of dissolution.
Metamorphic Conditions of Neotethyan Meliatic Accretionary Wedge Estimated by Thermodynamic Modelling and Geothermobarometry (Inner Western Carpathians)
Authors: Nemec Ondrej , Putiš Marián , Bačík Peter , Ružička Peter , Németh Zoltán
Published: December 2020

Metamorphic evolution of an accretionary wedge can be constrained by a reconstructed P–T conditions of the oceanic and continental margin fragments. This paper deals with the metamorphic overprinting of the Inner Western Carpathians (IWC) Meliatic Triassic–Jurassic paleotectonic units after the closure of the Neotethyan Meliata Basin. Medium to high-pressure and lower temperature conditions were estimated by Perple_X pseudosection modelling, combined with garnet–phengite, calcite–dolomite and chlorite thermometers and chlorite–phengite and phengite barometers. The Late Jurassic subductional burial to a maximum 50 km depth was estimated from the Bôrka Unit continental margin fragments at 520 °C and 1.55 GPa. This is compatible with the metamorphic peak garnet–glaucophane–phengite assemblage of blueschist facies in metabasites. The Jaklovce Unit oceanic fragments were subducted to maximum 35–40 km at 390–420 °C and 1.1–1.3 GPa. Metabasalts and metadolerites contain winchite, riebeckite, actinolite, chlorite, albite, epidote and phengite. A glaucophane-bearing metabasalt recorded an intra-oceanic subduction in blueschist-facies conditions. Rare amphibolite-facies metabasalts of this unit indicate the base of an inferred oceanic crust sliver obducted onto the continental margin wedge. The Meliata Unit oceanic/continental margin flysch calciclastic and siliciclastic metasediments suggest the burial to approximately 15–20 km at 250–350 °C and 0.4–0.6 GPa. This is indicated by a newly formed albite, K-feldspar, illite–phengite and chlorite associated with quartz and/or calcite and dolomite in these rocks. Magnesio-hastingsite to magnesio-hornblende bearing metagabbro with newly formed metamorphic magnesio-riebeckite and actinolite is an inferred detached Meliatic block tectonically emplaced in a Permian salinar mélange in the Silica Nappe hanging wall. Reconstructed P–T paths indicate variable metamorphic conditions from the medium-pressure to high-pressure subduction of the Bôrka and Jaklovce units to the Meliata Unit shallow burial in an accretionary wedge during Late Jurassic to Early Cretaceous Meliaticum evolution. Mélange blocks of Meliaticum incorporate different juxtaposed Meliatic paleotectonic units exposed in nappe outliers overlying the IWC Gemeric and Veporic superunits.
New Apatite Fission-Track Data from the Murmansk Craton, NE Fennoscandia: An Echo of Hidden Thermotectonic Events
Authors: Veselovskiy Roman V. , Arató Róbert , Bagdasaryan Tanya E. , Samsonov Alexander V. , Stepanova Alexandra V. , Arzamastsev Andrey A. , Myshenkova Mariya S.
Published: December 2020

For a long time, the thermal history of northeastern (NE) Fennoscandia in the Phanerozoic and Precambrian remained unknown, since no thermochronological studies were carried out within the Kola Peninsula area. Two years ago, we developed the first model of tectono-thermal evolution of the Kola Peninsula territory for the last 1.9 Gyr using a set of newly obtained apatite fission-track (AFT) and Ar/Ar thermochronological data. However, the low-temperature history of the most ancient tectonic unit of the northeastern part of the Kola Peninsula—the Archean Murmansk craton—remained poorly constrained due to the lack of AFT data. In this paper, we present the first results of AFT studies of 14 samples representing intrusive and metamorphic Precambrian rocks, located within the Murmansk craton of NE Fennoscandia. AFT ages and track length distributions indicate a similar tectono-thermal evolution of Precambrian tectonic units in NE Fennoscandia over the last 300 Myr. The AFT ages are distributed between ca. 177 and ca. 384 Ma; their median value, ~293 Ma, confirms the presence of a previously identified hidden thermal event that took place at about 300 Ma. However, a detailed analysis of the AFT age distribution shows the presence of three statistically distinguishable age components: 180–190 Ma (C1), 290–320 Ma (C2) and 422 Ma (C3). We assume that the relatively young AFT ages of C1 may originate from apatite crystals with low thermal resistivity. Remarkably, this value coincides with the initial stage of the Barents Sea magmatic province activity during large-scale plume-lithospheric interaction, as well as with the assumed age of an enigmatic remagnetization event throughout the Kola Peninsula. C2 ages can be observed in both the gabbroic and non-gabbroic samples, whereas C3 ages can only be found in gabbro. It is supposed that C2 ages, similarly to the Central Kola terrane, correspond to a cooling event related to the denudation of a thick sedimentary cover, representing a continuation of the Caledonian foreland basin towards NE Fennoscandia. C3 ages may be associated with a thermal event corresponding to the Caledonian collisional orogeny.
Sequential Bioleaching of Pyritic Tailings and Ferric Leaching of Nonferrous Slags as a Method for Metal Recovery from Mining and Metallurgical Wastes
Authors: Fomchenko Natalya , Muravyov Maxim
Published: December 2020

In this work, we proposed a method for biohydrometallurgical processing of mining (old pyritic flotation tailings) and metallurgical (slag) wastes to recover gold and other nonferrous metals. Since this processing allows the removal of toxic metals or at least decreases their content in the solids, this approach may reduce the negative environmental impacts of such waste. The proposed process was based on pyritic tailings’ bioleaching to recover metals and produce leach liquor containing a strong oxidizing agent (ferric sulfate) to dissolve nonferrous metal from slag. This approach also allows us to increase concentrations of nonferrous metals in the pregnant leach solution after pyritic waste bioleaching to allow efficient extraction. The old pyritic tailings were previously leached with 0.25% sulfuric acid for 10 min to remove soluble metal sulfates. As a result, 36% of copper and 35% of zinc were extracted. After 12 days of bioleaching with a microbial consortium containing Leptospirillum spp., Sulfobacillus spp., Ferroplasma spp., and Acidithiobacillus spp. at 35 °C, the total recovery of metals from pyritic tailings reached 68% for copper and 77% for zinc; and subsequent cyanidation allowed 92% recovery of gold. Ferric leaching of two types of slag at 70 °C with the leachate obtained during bioleaching of the tailings and containing 15 g/L of Fe3+ allowed 88.9 and 43.4% recovery of copper and zinc, respectively, from copper slag within 150 min. Meanwhile, 91.5% of copper, 84.1% of nickel, and 70.2% of cobalt were extracted from copper–nickel slag within 120 min under the same conditions.
Biodegradable Hydrogels Loaded with Magnetically Responsive Microspheres as 2D and 3D Scaffolds
Authors: Carvalho Estela O. , Ribeiro Clarisse , Correia Daniela M. , Botelho Gabriela , Lanceros-Mendez Senentxu
Published: December 2020

Scaffolds play an essential role in the success of tissue engineering approaches. Their intrinsic properties are known to influence cellular processes such as adhesion, proliferation and differentiation. Hydrogel-based matrices are attractive scaffolds due to their high-water content resembling the native extracellular matrix. In addition, polymer-based magnetoelectric materials have demonstrated suitable bioactivity, allowing to provide magnetically and mechanically activated biophysical electrical stimuli capable of improving cellular processes. The present work reports on a responsive scaffold based on poly (L-lactic acid) (PLLA) microspheres and magnetic microsphere nanocomposites composed of PLLA and magnetostrictive cobalt ferrites (CoFe2O4), combined with a hydrogel matrix, which mimics the tissue’s hydrated environment and acts as a support matrix. For cell proliferation evaluation, two different cell culture conditions (2D and 3D matrices) and two different strategies, static and dynamic culture, were applied in order to evaluate the influence of extracellular matrix-like confinement and the magnetoelectric/magneto-mechanical effect on cellular behavior. MC3T3-E1 proliferation rate is increased under dynamic conditions, indicating the potential use of hydrogel matrices with remotely stimulated magnetostrictive biomaterials for bone tissue engineering.
Stability of Quantum-Dot Light Emitting Diodes with Alkali Metal Carbonates Blending in Mg Doped ZnO Electron Transport Layer
Authors: Kim Hyo-Min , Jeong Wonkyeong , Kim Joo Hyun , Jang Jin
Published: December 2020

We report here the fabrication of highly efficient and long-lasting quantum-dot light emitting diodes (QLEDs) by blending various alkali metal carbonate in magnesium (Mg) doped zinc oxide (ZnO) (MZO) electron transport layer (ETL). Alkali metal carbonates blending in MZO, X2CO3:MZO, control the band-gap, electrical properties, and thermal stability. This can therefore enhance the operational lifetime of QLEDs. It is found that the conductivity of X2CO3:MZO film can be controlled and the thermal stability of ETLs could be improved by X2CO3 blending in MZO. The inverted red QLEDs (R-QLEDs) with Cs2CO3:MZO, Rb2CO3:MZO, and K2CO3:MZO ETLs exhibited the operational lifetime of 407 h for the R-QLEDs with Cs2CO3:MZO, 620 h with Rb2CO3:MZO and 94 h with K2CO3:MZO ETLs at T95 with the initial luminance of 1000 cd/m2. Note that all red QLEDs showed the high brightness over 150,000 cd/m2. But the R-QLEDs with Na2CO3:MZO and Li2CO3:MZO ETLs exhibited shorter operational lifetime and poor brightness than the R-QLED with pristine MZO ETL.
The Effects of a Varied Gold Shell Thickness on Iron Oxide Nanoparticle Cores in Magnetic Manipulation, T1 and T2 MRI Contrasting, and Magnetic Hyperthermia
Authors: Brennan Grace , Bergamino Silvia , Pescio Martina , Tofail Syed A. M. , Silien Christophe
Published: December 2020

Fe3O4–Au core–shell magnetic-plasmonic nanoparticles are expected to combine both magnetic and light responsivity into a single nanosystem, facilitating combined optical and magnetic-based nanotheranostic (therapeutic and diagnostic) applications, for example, photothermal therapy in conjunction with magnetic resonance imaging (MRI) imaging. To date, the effects of a plasmonic gold shell on an iron oxide nanoparticle core in magnetic-based applications remains largely unexplored. For this study, we quantified the efficacy of magnetic iron oxide cores with various gold shell thicknesses in a number of popular magnetic-based nanotheranostic applications; these included magnetic sorting and targeting (quantifying magnetic manipulability and magnetophoresis), MRI contrasting (quantifying benchtop nuclear magnetic resonance (NMR)-based T1 and T2 relaxivity), and magnetic hyperthermia therapy (quantifying alternating magnetic-field heating). We observed a general decrease in magnetic response and efficacy with an increase of the gold shell thickness, and herein we discuss possible reasons for this reduction. The magnetophoresis speed of iron oxide nanoparticles coated with the thickest gold shell tested here (ca. 42 nm) was only ca. 1% of the non-coated bare magnetic nanoparticle, demonstrating reduced magnetic manipulability. The T1 relaxivity, r1, of the thick gold-shelled magnetic particle was ca. 22% of the purely magnetic counterpart, whereas the T2 relaxivity, r2, was 42%, indicating a reduced MRI contrasting. Lastly, the magnetic hyperthermia heating efficiency (intrinsic loss power parameter) was reduced to ca. 14% for the thickest gold shell. For all applications, the efficiency decayed exponentially with increased gold shell thickness; therefore, if the primary application of the nanostructure is magnetic-based, this work suggests that it is preferable to use a thinner gold shell or higher levels of stimuli to compensate for losses associated with the addition of the gold shell. Moreover, as thinner gold shells have better magnetic properties, have previously demonstrated superior optical properties, and are more economical than thick gold shells, it can be said that “less is more”.
Electrical Conduction Behavior of High-Performance Microcellular Nanocomposites Made of Graphene Nanoplatelet-Filled Polysulfone
Authors: Abbasi Hooman , Antunes Marcelo , Velasco José
Published: December 2020

Graphene nanoplatelet (GnP)-filled polysulfone (PSU) cellular nanocomposites, prepared by two different methods—namely, water vapor-induced phase separation (WVIPS) and supercritical CO2 dissolution (scCO2) foaming—were produced with a range of densities from 0.4 to 0.6 g/cm3 and characterized in terms of their structure and electrical conduction behavior. The GnP content was varied from 0 to 10 wt%. The electrical conductivity values were increased with the amount of GnP for the three different studied foam series. The highest values were found for the microcellular nanocomposites prepared by the WVIPS method, reaching as high as 8.17 × 10−2 S/m for 10 wt% GnP. The variation trend of the electrical conductivity for each series was analyzed by applying both the percolation and the tunneling models. Comparatively, the tunneling model showed a better fitting in the prediction of the electrical conductivity. The preparation technique of the cellular nanocomposite affected the resultant cellular structure of the nanocomposite and, as a result, the porosity or gas volume fraction (Vg). A higher porosity resulted in a higher electrical conductivity, with the lightest foams being prepared by the WVIPS method, showing electrical conductivities two orders of magnitude higher than the equivalent foams prepared by the scCO2 dissolution technique.
Tunable Magnetic Hyperthermia Properties of Pristine and Mildly Reduced Graphene Oxide/Magnetite Nanocomposite Dispersions
Authors: Illés Erzsébet , Tombácz Etelka , Hegedűs Zsófia , Szabó Tamás
Published: December 2020

We present a study on the magnetic hyperthermia properties of graphene oxide/magnetite (GO/MNP) nanocomposites to investigate their heat production behavior upon the modification of the oxidation degree of the carbonaceous host. Avoiding the harsh chemical conditions of the regular in situ co-precipitation-based routes, the oppositely charged MNPs and GO nanosheets were combined by the heterocoagulation process at pH ~ 5.5, which is a mild way to synthesize composite nanostructures at room temperature. Nanocomposites prepared at 1/5 and 1/10 GO/MNP mass ratios were reduced by NaBH4 and L-ascorbic acid (LAA) under acidic (pH ~ 3.5) and alkaline conditions (pH ~ 9.3). We demonstrate that the pH has a crucial effect on the LAA-assisted conversion of graphene oxide to reduced GO (rGO): alkaline reduction at higher GO loadings leads to doubled heat production of the composite. Spectrophotometry proved that neither the moderately acidic nor alkaline conditions promote the iron dissolution of the magnetic core. Although the treatment with NaBH4 also increased the hyperthermic efficiency of aqueous GO/MNP nanocomposite suspensions, it caused a drastic decline in their colloidal stability. However, considering the enhanced heat production and the slightly improved stability of the rGO/MNP samples, the reduction with LAA under alkaline condition is a more feasible way to improve the hyperthermic efficiency of magnetically modified graphene oxides.
Laser Printing of Plasmonic Nanosponges
Authors: Syubaev Sergey , Gurbatov Stanislav , Modin Evgeny , Linklater Denver P. , Juodkazis Saulius , Gurevich Evgeny L. , Kuchmizhak Aleksandr
Published: December 2020

Three-dimensional porous nanostructures made of noble metals represent novel class of nanomaterials promising for nonlinear nanooptics and sensors. Such nanostructures are typically fabricated using either reproducible yet time-consuming and costly multi-step lithography protocols or less reproducible chemical synthesis that involve liquid processing with toxic compounds. Here, we combined scalable nanosecond-laser ablation with advanced engineering of the chemical composition of thin substrate-supported Au films to produce nanobumps containing multiple nanopores inside. Most of the nanopores hidden beneath the nanobump surface can be further uncapped using gentle etching of the nanobumps by an Ar-ion beam to form functional 3D plasmonic nanosponges. The nanopores 10–150 nm in diameter were found to appear via laser-induced explosive evaporation/boiling and coalescence of the randomly arranged nucleation sites formed by nitrogen-rich areas of the Au films. Density of the nanopores can be controlled by the amount of the nitrogen in the Au films regulated in the process of their magnetron sputtering assisted with nitrogen-containing discharge gas.
Enhancing the Efficiency of Color Conversion Micro-LED Display with a Patterned Cholesteric Liquid Crystal Polymer Film
Authors: Hsiang En-Lin , Li Yannanqi , He Ziqian , Zhan Tao , Zhang Caicai , Lan Yi-Fen , Dong Yajie , Wu Shin-Tson
Published: December 2020

Color-converted micro-light-emitting diode (micro-LED) displays with wide color gamut, high ambient contrast ratio, and fast response time are emerging as a potentially disruptive technology. However, due to limited optical density and thickness of the color-conversion film, the blue light leakage and low color-conversion efficiency still hinder their widespread applications. In this paper, we demonstrate a patterned cholesteric liquid crystal (CLC) polymer film with two special optical functionalities. On the green and red sub-pixels, the corresponding planar CLC texture acts as a distributed Bragg reflector for the blue light, which in turn improves the color conversion efficiency and expands the color gamut. On the blue sub-pixels, the corresponding focal-conic CLC texture acts as light scattering medium, which helps to reduce the angular color shift. Further analysis reveals that the patterned CLC film can alleviate the crosstalk between green and blue color filters. Therefore, compared to the display system without such a CLC film, our proposed device structure increases the color conversion efficiency by 143% (at ~90% Rec. 2020) and reduces average angular color shift Δu’v’ from 0.03 to 0.018 at the viewing angle with the most severe color shift. Such a patterned CLC film is applicable to all kinds of color-conversion display systems, including organic and inorganic phosphors.
Boron Nitride Nanotubes Versus Carbon Nanotubes: A Thermal Stability and Oxidation Behavior Study
Authors: Kostoglou Nikolaos , Tampaxis Christos , Charalambopoulou Georgia , Constantinides Georgios , Ryzhkov Vladislav , Doumanidis Charalabos , Matovic Branko , Mitterer Christian , Rebholz Claus
Published: December 2020

Nanotubes made of boron nitride (BN) and carbon have attracted considerable attention within the literature due to their unique mechanical, electrical and thermal properties. In this work, BN and carbon nanotubes, exhibiting high purity (>99%) and similar surface areas (~200 m2/g), were systematically investigated for their thermal stability and oxidation behavior by combining thermal gravimetric analysis and differential scanning calorimetry methods at temperatures of up to ~1300 °C under a synthetic air flow environment. The BN nanotubes showed a good resistance to oxidation up to ~900 °C and fully transformed to boron oxide up to ~1100 °C, while the carbon nanotubes were stable up to ~450 °C and almost completely combusted up to ~800 °C. The different oxidation mechanisms are attributed to the different chemical nature of the two types of nanotubes.
Influence of PDA Coating on the Structural, Optical and Surface Properties of ZnO Nanostructures
Authors: Damberga Daina , Fedorenko Viktoriia , Grundšteins Kārlis , Altundal Şahin , Šutka Andris , Ramanavičius Arunas , Coy Emerson , Mrówczyński Radosław , Iatsunskyi Igor , Viter Roman
Published: December 2020

Polydopamine (PDA) is a new biocompatible material, which has prospects in biomedical and sensor applications. Due to functional groups, it can host wide range of biomolecules. ZnO nanostructures are well known templates for optical sensors and biosensors. The combination of ZnO and PDA results in a change of optical properties of ZnO–PDA composites as a shift of photoluminescence (PL) peaks and PL quenching. However, to date, the effect of the PDA layer on fundamental properties of ZnO–PDA nanostructures has not been studied. The presented paper reports on optical and surface properties of novel ZnO–PDA nanocomposites. PDA layers were chemically synthesized on ZnO nanostructures from different solution concentrations of 0.3, 0.4, 0.5 and 0.7 mg/mL. Structure, electronic and optical properties were studied by SEM, Raman, FTIR, diffuse reflectance and photoluminescence methods. The Z-potential of the samples was evaluated in neutral pH (pH = 7.2). The response of the samples towards poly-l-lysine adsorption, as a model molecule, was studied by PL spectroscopy to evaluate the correlation between optical and surface properties. The role of the PDA concentration on fundamental properties was discussed.
Ultrafast Yb-Doped Fiber Laser Using Few Layers of PdS2 Saturable Absorber
Authors: Cheng Ping Kwong , Liu Shunxiang , Ahmed Safayet , Qu Junle , Qiao Junpeng , Wen Qiao , Tsang Yuen Hong
Published: December 2020

Two-dimensional (2D) transition metal dichalcogenide (TMD) materials have exceptional optoelectronic and structural properties, which allow them to be utilized in several significant applications in energy, catalyst, and high-performance optoelectronic devices. Among other properties, the nonlinear optical properties are gaining much attention in the research field. In this work, a unique pentagonal TMD material, palladium disulfide (PdS2), is employed as a saturable absorber (SA) in an ytterbium-doped fiber (YDF) laser cavity and mode-locked laser pulse is generated. At first, liquid phase exfoliation is performed to prepare PdS2 nanoflakes. Afterward, the PdS2-nanoflakes solution was incorporated in the side-polished fiber (SPF) to form SPF-based PdS2-SA. By utilizing this SA, a highly stable mode-locked laser pulse is realized at pump power of 160 mW, which has a center wavelength of 1033 nm and a 3-dB spectral bandwidth of 3.7 nm. Moreover, the pulse duration, maximum power output and corresponding single-pulse energy were determined as 375 ps, 15.7 mW and 0.64 nJ, respectively. During the experiment, the mode-locked pulse remained stable till the pump power reached a value of 400 mW and, for the regulation of power, the slope efficiency is calculated at about 4.99%. These results indicate that PdS2 material is a promising nonlinear optical material for ultrafast optical applications in the near-infrared (NIR) region.
Improving the Intercellular Uptake and Osteogenic Potency of Calcium Phosphate via Nanocomplexation with the RALA Peptide
Authors: O’Doherty Michelle , Mulholland Eoghan J. , Chambers Philip , Pentlavalli Sreekanth , Ziminska Monika , Chalanqui Marine J. , Pauly Hannah M. , Sathy Binulal N. , Donahue Tammy H. , Kelly Daniel J. , Dunne Nicholas , McCarthy Helen O.
Published: December 2020

Calcium phosphate-base materials (e.g., alpha tri-calcium phosphate (α–TCP)) have been shown to promote osteogenic differentiation of stem/progenitor cells, enhance osteoblast osteogenic activity and mediate in vivo bone tissue formation. However, variable particle size and hydrophilicity of the calcium phosphate result in an extremely low bioavailability. Therefore, an effective delivery system is required that can encapsulate the calcium phosphate, improve cellular entry and, consequently, elicit a potent osteogenic response in osteoblasts. In this study, collagenous matrix deposition and extracellular matrix mineralization of osteoblast lineage cells were assessed to investigate osteogenesis following intracellular delivery of α-TCP nanoparticles. The nanoparticles were formed via condensation with a novel, cationic 30 mer amphipathic peptide (RALA). Nanoparticles prepared at a mass ratio of 5:1 demonstrated an average particle size of 43 nm with a zeta potential of +26 mV. The average particle size and zeta potential remained stable for up to 28 days at room temperature and across a range of temperatures (4–37 °C). Cell viability decreased 24 h post-transfection following RALA/α-TCP nanoparticle treatment; however, recovery ensued by Day 7. Immunocytochemistry staining for Type I collagen up to Day 21 post-transfection with RALA/α-TCP nanoparticles (NPs) in MG-63 cells exhibited a significant enhancement in collagen expression and deposition compared to an untreated control. Furthermore, in porcine mesenchymal stem cells (pMSCs), there was enhanced mineralization compared to α–TCP alone. Taken together these data demonstrate that internalization of RALA/α-TCP NPs elicits a potent osteogenic response in both MG-63 and pMSCs.
Grain Intake and Human Health
Authors: Zingone Fabiana
Published: December 2020

Wheat is one of the most consumed cereal grains worldwide and represents an important part of the human diet [...]
Bartonella Associated Cutaneous Lesions (BACL) in People with Neuropsychiatric Symptoms
Authors: Breitschwerdt Edward B. , Bradley Julie M. , Maggi Ricardo G. , Lashnits Erin , Reicherter Paul
Published: December 2020

Bartonella species are globally important emerging pathogens that were not known to infect animals or humans in North America prior to the human immunodeficiency virus (HIV) epidemic. Ongoing improvements in diagnostic testing modalities have allowed for the discovery of Bartonella species (spp.) DNA in blood; cerebrospinal fluid; and the skin of patients with cutaneous lesions, fatigue, myalgia, and neurological symptoms. We describe Bartonella spp. test results for participants reporting neuropsychiatric symptoms, the majority of whom reported the concurrent development of cutaneous lesions. Study participants completed a medical history, a risk factor questionnaire, and provided cutaneous lesion photographs. Bartonella spp. serology and Bartonella alpha proteobacteria enrichment blood culture/PCR were assessed. Within a 14-month period, 33 participants enrolled; 29/33 had serological and/or PCR evidence supporting Bartonella spp. infection, of whom 24 reported concurrent cutaneous lesions since neuropsychiatric symptom onset. We conclude that cutaneous lesions were common among people reporting neuropsychiatric symptoms and Bartonella spp. infection or exposure. Additional studies, using sensitive microbiological and imaging techniques, are needed to determine if, or to what extent, Bartonella spp. might contribute to cutaneous lesions and neuropsychiatric symptoms in patients.
Further Evidence of Possible Therapeutic Uses of Sambucus nigra L. Extracts by the Assessment of the In Vitro and In Vivo Anti-Inflammatory Properties of Its PLGA and PCL-Based Nanoformulations
Authors: Mota Ana Henriques , Duarte Noélia , Serra Ana Teresa , Ferreira António , Bronze Maria Rosário , Custódio Luísa , Gaspar Maria Manuela , Simões Sandra , Rijo Patrícia , Ascensão Lia , Faísca Pedro , Viana Ana Silveira , Pinto Rui , Kumar Pradeep , Almeida António José , Reis Catarina Pinto
Published: December 2020

Sambucus nigra L. is widely used in traditional medicine with different applications. However, confirmative studies are strongly required. This study aimed to assess the biological activities of the S. nigra flower’s extract encapsulated into two different types of nanoparticles for optimizing its properties and producing further evidence of its potential therapeutic uses. Different nanoparticles (poly(lactide-co-glycolide, PLGA) and poly-Ɛ-caprolactone (PCL), both with oleic acid, were prepared by emulsification/solvent diffusion and solvent-displacement methods, respectively. Oleic acid was used as a capping agent. After the nanoparticles’ preparation, they were characterized and the biological activities were studied in terms of collagenase, in vitro and in vivo anti-inflammatory, and in vitro cell viability. Rutin and naringenin were found to be the major phenolic compounds in the studied extract. The encapsulation efficiency was higher than 76% and revealed to have an impact on the release of the extract, mainly for the PLGA. Moreover, biochemical and histopathological analyses confirmed that the extract-loaded PLGA-based nanoparticles displayed the highest anti-inflammatory activity. In addition to supporting the previously reported evidence of potential therapeutic uses of S. nigra, these results could draw the pharmaceutical industry’s interest to the novelty of the nanoproducts.