bluuebunny/crossref_metadata_2025 huggingface dataset:
A Review of Medicinal Plants with Renoprotective Activity in Diabetic Nephropathy Animal Models
Authors: Putra I Made Wisnu Adhi , Fakhrudin Nanang , Nurrochmad Arief , Wahyuono Subagus
Published: February 2023

Diabetic nephropathy (DN), also recognized as diabetic kidney disease, is a kidney malfunction caused by diabetes mellitus. A possible contributing factor to the onset of DN is hyperglycemia. Poorly regulated hyperglycemia can damage blood vessel clusters in the kidneys, leading to kidney damage. Its treatment is difficult and expensive because its causes are extremely complex and poorly understood. Extracts from medicinal plants can be an alternative treatment for DN. The bioactive content in medicinal plants inhibits the progression of DN. This work explores the renoprotective activity and possible mechanisms of various medicinal plant extracts administered to diabetic animal models. Research articles published from 2011 to 2022 were gathered from several databases including PubMed, Scopus, ProQuest, and ScienceDirect to ensure up-to-date findings. Results showed that medicinal plant extracts ameliorated the progression of DN via the reduction in oxidative stress and suppression of inflammation, advanced glycation end-product formation, cell apoptosis, and tissue injury-related protein expression.
Knowledge-Based Human Resource Management, Logistics Capability, and Organizational Performance in Small Finnish Logistics Service Providers
Authors: Evangelista Pietro , Kianto Aino , Hussinki Henri , Vanhala Mika , Nisula Anna-Maija
Published: February 2023

Background: In the current knowledge age, the performance of logistics firms increasingly depends on knowledge and capabilities they can leverage in value-creation processes. The objective of this paper is to investigate the relationship between knowledge-based human resource management (HRM) practices and organizational performance mediated by logistics capability. Methods: Data from 93 small Finnish logistics service providers was collected by a questionnaire survey. Structural equation modeling and partial least squares analysis were used to test the hypothesized relationships between HRM practices and the organizational performance of logistics companies, as well as the mediating impact of logistics capability. Results: The results demonstrate that logistics capability fully mediates the relationship between HRM practices and the organizational performance of small logistics companies. Each of the three knowledge-based HRM practices—knowledge-based recruitment, training and development, and motivation—significantly influences logistics capability, which is, in turn, associated with the organizational performance of companies. Conclusions: This paper is one of the few among discussing knowledge-based HRM practices in logistics service research. The paper contributes to the literature on logistics by demonstrating the value of knowledge-based HRM practices in acquiring, developing, and leveraging knowledge to create organizational capabilities and ensure organizational performance. This study provides an improved understanding of the role of HRM and the resulting logistics capability in the performance of small logistics service companies.
A Study on the Effects of Hybridized Metal Oxide and Carbonaceous Nano-Cutting Fluids in the End Milling of AA6082 Aluminum Alloy
Authors: S Vignesh , U Mohammed Iqbal , Ghani Jaharah A.
Published: February 2023

Finding an alternate solution for supplanting the existing conventional lubricant in machining is a challenge. This work narrows the search down to the use of nano-cutting fluids, as they exhibit excellent properties such as high thermal conductivity and good lubricity. A technical analysis of the performance of hybrid nano-cutting fluids in the end milling of AA6082 aluminium alloy in a constrained end milling condition is presented. Alumina and carbon nanotubes were chosen in this study for their better physical characteristics and compatibility during machining. Coconut oil was chosen as the base fluid (dispersal medium) as it provides good lubricity and better dispersion of nanoparticles due to its excellent rheological behaviour. The hybrid nanofluid was prepared by mixing alumina-based nanofluid with carbon nanotube nanoparticles in different volumetric concentrations. The thermo-physical properties of the prepared hybrid nanofluid were tested. Furthermore, they were tested for their spread-ability and other mechanical properties. Later, their performances as cutting fluid were studied with the minimum quantity lubrication (MQL) technique, wherein nanoparticle mist was formed and evaluated in the end milling of AA6082 aluminium to reduce the quantity of nanofluids’ usage during end milling. The controllable parameters of speed, feed rate, and type of cutting fluid were chosen, with the levels of cutting speeds and feed rate at 75–125 m/min, and 0.005–0.015 mm/tooth, respectively, and the response parameters studied were surface roughness and tool wear. The results show that better performance is achieved in hybridized nano-cutting fluid, with a sharp improvement of 20%, and 25% in tool wear and surface roughness when compared to the base fluid. This study has explored the concept of hybridization and the capability of nanofluids as cutting fluids that can be used as eco-friendly cutting fluids in manufacturing industries.
Phenomenological Laws of Single Point Contact: Pre-Rolling Contact Resistance through Pendulum
Authors: Gilavdary Igor. , Mekid Samir , Riznookaya Natalia.
Published: February 2023

The development results of a single-point contact system set up as a pendulum to study the laws of rolling resistance to contacting bodies at a distance significantly reduced compared to the elastic contact spot size. The designed device uses a physical pendulum sustained by only one ball on a flat polished surface. The problem of stability of the pendulum swing plane is solved. A phenomenological theory of rolling resistance is described. The surface tension of solids on the contact zone, parameters of the frequency-independent internal friction and the pressure of the adhesion forces are found.
Thermal-Fluid-Solid Coupling Simulation and Oil Groove Structure Optimization of Wet Friction Clutch for High-Speed Helicopter
Authors: Tan Wuzhong , Chen Zhi , Li Zhizuo , Yan Hongzhi
Published: February 2023

Wet friction clutch is the key functional component of the high-speed helicopter variable-speed transmission system, which is used to change the power transmission path. In the engagement process of wet friction clutch, the driving/driven disc will produce drag torque under the shearing of lubricating oil, which reduces the transmission efficiency. This unnecessary drag torque reduces efficiency and increases clutch temperature. The temperature increase promotes the wear of gears and bearings and the aging deformation of friction plates, which leads to local wear and reduces the service life of the clutch. From the principle of wet friction clutch, the oil groove structure is directly related to the drag torque and the temperature rise of friction disc. It is very important for the long-distance flight and service life of high-speed helicopters to obtain the groove structure with low drag torque and low temperature rise. In order to solve this problem, taking the wet friction clutch of a high-speed helicopter as the research object, based on the radial and annular compound groove, the thermal-fluid-solid coupling simulation model of the wet friction clutch is established to obtained the flow characteristics and temperature field distribution of the lubricating oil in the friction disc oil groove, and to analyze the influence law of the oil groove structure parameters on the drag torque and temperature field. In order to improve the transmission efficiency and the service life of friction disc, Taguchi experiment and non-dominated neighborhood immune algorithm were used to optimize the structural parameters of the oil grooves. The comparison results show that the optimized structural can effectively reduce the drag torque and the temperature rise. This work can provide a theoretical reference for the structure design of a wet friction clutch.
Robust and Secure Quality Monitoring for Welding through Platform-as-a-Service: A Resistance and Submerged Arc Welding Study
Authors: Stavropoulos Panagiotis , Papacharalampopoulos Alexios , Sabatakakis Kyriakos
Published: February 2023

For smart manufacturing systems, quality monitoring of welding has already started to shift from empirical modeling to knowledge integration directly from the captured data by utilizing one of the most promising Industry 4.0’s key enabling technologies, artificial intelligence (AI)/machine learning (ML). However, beyond the advantages that they bring, AI/ML introduces new types of security threats, which are related to their very nature and eventually, they will pose real threats to the production cost and quality of products. These types of security threats, such as adversarial attacks, are causing the targeted AI system to produce incorrect or malicious outputs. This may undermine the performance (and the efficiency) of the quality monitoring systems. Herein, a software platform servicing quality monitoring for welding is presented in the context of resistance and submerged arc welding. The hosted ML classification models that are trained to perform quality monitoring are subjected to two different types of untargeted, black-box, adversarial attacks. The first one is based on a purely statistical approach and the second one is based on process knowledge for crafting these adversarial inputs that can compromise the models’ accuracy. Finally, the mechanisms upon which these adversarial attacks are inflicting damage are discussed to identify which process features or defects are replicated. This way, a roadmap is sketched toward testing the vulnerability and robustness of an AI-based quality monitoring system.
Can Principal Component Analysis Be Used to Explore the Relationship of Rowing Kinematics and Force Production in Elite Rowers during a Step Test? A Pilot Study
Authors: Jensen Matt , Stellingwerff Trent , Pollock Courtney , Wakeling James , Klimstra Marc
Published: February 2023

Investigating the relationship between the movement patterns of multiple limb segments during the rowing stroke on the resulting force production in elite rowers can provide foundational insight into optimal technique. It can also highlight potential mechanisms of injury and performance improvement. The purpose of this study was to conduct a kinematic analysis of the rowing stroke together with force production during a step test in elite national-team heavyweight men to evaluate the fundamental patterns that contribute to expert performance. Twelve elite heavyweight male rowers performed a step test on a row-perfect sliding ergometer [5 × 1 min with 1 min rest at set stroke rates (20, 24, 28, 32, 36)]. Joint angle displacement and velocity of the hip, knee and elbow were measured with electrogoniometers, and force was measured with a tension/compression force transducer in line with the handle. To explore interactions between kinematic patterns and stroke performance variables, joint angular velocities of the hip, knee and elbow were entered into principal component analysis (PCA) and separate ANCOVAs were run for each performance variable (peak force, impulse, split time) with dependent variables, and the kinematic loading scores (Kpc,ls) as covariates with athlete/stroke rate as fixed factors. The results suggested that rowers’ kinematic patterns respond differently across varying stroke rates. The first seven PCs accounted for 79.5% (PC1 [26.4%], PC2 [14.6%], PC3 [11.3%], PC4 [8.4%], PC5 [7.5%], PC6 [6.5%], PC7 [4.8%]) of the variances in the signal. The PCs contributing significantly (p ≤ 0.05) to performance metrics based on PC loading scores from an ANCOVA were (PC1, PC2, PC6) for split time, (PC3, PC4, PC5, PC6) for impulse, and (PC1, PC6, PC7) for peak force. The significant PCs for each performance measure were used to reconstruct the kinematic patterns for split time, impulse and peak force separately. Overall, PCA was able to differentiate between rowers and stroke rates, and revealed features of the rowing-stroke technique correlated with measures of performance that may highlight meaningful technique-optimization strategies. PCA could be used to provide insight into differences in kinematic strategies that could result in suboptimal performance, potential asymmetries or to determine how well a desired technique change has been accomplished by group and/or individual athletes.
Serum YKL-40 Levels in Patients with Asthma or COPD: A Pilot Study
Authors: Popețiu Romana Olivia , Donath-Miklos Imola , Borta Simona Maria , Moldovan Silviu Daniel , Pilat Luminița , Nica Dragoș Vasile , Pușchiță Maria
Published: February 2023

Background and Objectives: Bronchial asthma (BA) and chronic obstructive pulmonary disease (COPD) are not only common obstructive respiratory conditions but also major causes of morbidity and mortality worldwide. There is, however, a surprising lack of blood-based biomarkers for separating between these pulmonary disorders. The aim of this study was to assess the practical relevance of using serum YKL-40, single or combined, for this purpose. Materials and Methods: Subjects included Romanian patients with BA (n = 24) or COPD (n = 27). YKL-40, fibrinogen, pre-treatment C-reactive protein (CRP), post-treatment CRP, erythrocyte sedimentation rate, interleukin 6 (IL-6), procalcitonin (PCT), absolute neutrophil count, neutrophil percentage, absolute lymphocyte count, lymphocyte percentage, absolute eosinophil count, and eosinophil percentage were measured and compared between these patients. Results: This is the first study investigating the clinical significance of serum YKL-40 in delineating between COPD and BA in Caucasian populations. Only fibrinogen and YKL-40 levels were different between COPD and BA, with the measured values being significantly elevated. These patients exhibited distinct inflammatory profiles. Using the upper quartiles of these variables for the pooled study population (YKL-40: 5100 pg/mL; fibrinogen: 552 mg/dL) as cut-off values, subjects were classified into high or low groups. High YKL-40 adults revealed significantly increased PCT levels. High fibrinogen subjects, by contrast, showed significantly elevated IL-6 concentrations and pre-treatment CRP levels. Low YKL-40 and fibrinogen patients showed the absence of COPD. Conclusions: Combined use of serum YKL-40 and fibrinogen may be useful for identifying the absence of COPD.
SGLT2 Inhibitors: The Next Blockbuster Multifaceted Drug?
Authors: Chan Jonathan C. H. , Chan Michael C. Y.
Published: February 2023

Sodium glucose cotransporter 2 inhibitor (SGLT2i) is a class of drugs that were originally intended for decreasing blood glucose in diabetes. However, recent trials have shown that there are other beneficial effects. Major clinical trials involving SGLT2i medications from 2015 to 2022 were reviewed using PUBMED search. Recent major SGLT2i landmark trials have demonstrated benefits for cardiovascular disease (reduce major adverse cardiovascular events (heart attack, stroke, cardiovascular death), hospitalization for heart failure, all-cause death), and renal disease (delay the onset of dialysis) regardless of diabetic status. The consistent cardiorenal benefits observed in major landmark trials have resulted in the rapid adoption of SGLT2i therapy not only in diabetes guidelines but also cardiovascular and renal guidelines.
Role of Fat-Free Mass Index on Amino Acid Loss during CRRT in Critically Ill Patients
Authors: Vicka Vaidas , Vickiene Alvita , Miskinyte Sigute , Bartuseviciene Ieva , Lisauskiene Ingrida , Serpytis Mindaugas , Ringaitiene Donata , Sipylaite Jurate
Published: February 2023

Background and objectives: Amino acid (AA) loss is a prevalent unwanted effect of continuous renal replacement therapy (CRRT) in critical care patients, determined both by the machine set-up and individual characteristics. The aim of this study was to evaluate the bioelectrical impedance analysis-derived fat-free mass index (FFMI) effect on amino acid loss. Materials and methods: This was a prospective, observational, single sample study of critical care patients upon initiation of CRRT. AA loss during a 24 h period was estimated. Conventional determinants of AA loss (type and dose of CRRT, concentration of AA) and FFMI were entered into the multivariate regression analysis to determine the individual predictive value. Results: Fifty-two patients were included in the study. The average age was 66.06 ± 13.60 years; most patients had a high mortality risk with APAHCE II values of 22.92 ± 8.15 and SOFA values of 12.11 ± 3.60. Mean AA loss in 24 h was 14.73 ± 9.83 g. There was a significant correlation between the lost AA and FFMI (R = 0.445, B = 0.445 CI95%: 0.541–1.793 p = 0.02). Multivariate regression analysis revealed the independent predictors of lost AA to be the systemic concentration of AA (B = 6.99 95% CI:4.96–9.04 p = 0.001), dose of CRRT (B = 0.48 95% CI:0.27–0.70 p < 0.001) and FFMI (B = 0.91 95% CI:0.42–1.41 p < 0.001). The type of CRRT was eliminated in the final model due to co-linearity with the dose of CRRT. Conclusions: A substantial amount of AA is lost during CRRT. The amount lost is increased by the conventional factors as well as by higher FFMI. Insights from our study highlight the FFMI as a novel research object during CRRT, both when prescribing the dosage and evaluating the nutritional support needed.
Lignin Recovery from Black Liquor Using Integrated UF/NF Processes and Economic Analysis
Authors: Sharma Manorma , Alves Patrícia , Gando-Ferreira Licínio
Published: February 2023

Lignin is a polyphenolic biopolymer present in large amounts in black liquor (BL). This work investigated the recovery of lignin from BL (pre-filtered by ultrafiltration (UF)) by nanofiltration (NF). For the NF tests, laboratory-made mixed matrix membrane (MMM) prepared with 0.1% activated carbon (AC) nanoparticles were used in crossflow filtration mode. The effect of pressure (6–15 bar) and volume reduction (VR) (~65%) were analyzed, and the filtration performance was evaluated in terms of permeate flux, lignin rejection rate, and flux reduction. The lignin rejection rate varied in the range of 67–80% with the pressure, however, the highest increases in flux and rejection were observed at 12 bar, which was found to be the optimum pressure. At a VR of ~65%, the permeate flux decreased by ~55% and the lignin rejection rate increased from 78% to 86%. In addition, an economic evaluation was performed for the preparation of UF and NF MMM. The minimum-to-maximum price range was estimated considering the costs of the laboratory and commercial grade regents. It showed a difference of ~10-fold and ~14-fold for UF and NF membranes, respectively. The results of the laboratory-scale study were used to evaluate the economic feasibility of the process for recovering lignin- and hemicellulose-rich retentate streams.
The Cation Effect on the Free Volume and the Solubility of H2S and CO2 in Ionic Liquids Based on Bis(2-Ethylhexyl) Sulfosuccinate Anion
Authors: Sazanova Tatyana S. , Akhmetshina Alsu I. , Petukhov Anton N. , Vorotyntsev Andrey V. , Suvorov Sergey S. , Barysheva Alexandra V. , Mechergui Amal , Nyuchev Alexander V. , Kazarina Olga V. , Stepakova Anna N. , Atlaskina Maria E. , Atlaskin Artem A. , Kryuchkov Sergey S. , Vorotyntsev Ilya V.
Published: February 2023

Herein, we report for the first time a study dedicated to acidic gases’ solubility in ionic liquids with sterically hindered bulky anion, namely bis(2-ethylhexyl) sulfosuccinate ([doc]), experimentally evaluated at low pressures. The effect of cation change (imidazolium, pyridinium, and pyrrolidinium) on the thermophysical properties and sorption capacities was also discussed. The densities and the activation energies of the tested ILs exhibited minor differences. Furthermore, the COSMO-RS model was used to predict the free volumes of ILs aiming to investigate its influence on gas solubilities. The conducted calculations have revealed an antibate correlation between the fractional free volume (FFV) and Henry’s law constant. In particular, the lowest FFV in 1-methylimidazolium [doc] corresponded to the minimal sorption and vice versa. In addition, it was shown that the presence of protic cation results in a significant reduction in CO2 and H2S solubilities. In general, the solubility measurement results of the synthesized ILs have shown their superiority compared to fluorinated ILs based on the physical absorption mechanism.
High-Antimicrobial Gallium-Doped Zinc Oxide Thin Films on Bio-Based Poly(Ethylene Furanoate) Substrates for Food Packaging Application
Authors: Zhu Chaoting , Ye Danling , Zhou Tianqi , Cui Yashuang , Yin Jianbing
Published: February 2023

Thin films of gallium-doped zinc oxide (GZO), with a thickness of around fifty nanometers were deposited on bio-based poly(ethylene furanoate) (PEF) substrates by radio-frequency sputtering. By optimizing the Ga concentration in the target, the optics, water vapor barrier and antibacterial properties of PEF/GZO composite films can be adjusted. The highest visible light transmittance of the samples was around 85.1%. Furthermore, by introducing some GZO films with typical concentrations, the water vapor barrier and antibacterial properties of PEF films were improved. The optimized water vapor permeability of PEF/GZO composite film was 5.3 × 10−12 g·m/m2·s·Pa, and the highest antibacterial rate can reach 99.85% after 4 h. By XPS analysis, the antibacterial mechanism in the samples is envisaged to be mainly due cytotoxicity of Ga ions. The above results indicate that PEF/GZO films have great potential in the field of antibacterial food packaging.
Effect of Tool Rotation Direction on Mechanical Strength of Single Lap Friction Stir Welded Joints between AA5083 Aluminum Alloy and S355J0 Steel for Maritime Applications
Authors: Di Bella Guido , Borsellino Chiara , Khaskhoussi Amani , Proverbio Edoardo
Published: February 2023

This study aims to investigate a friction stir welded joint between steel and aluminum alloy that is employed in maritime applications (i.e., connection between the ship over-structures and the hull or deck). By changing the tool rotational direction, or the advancing or retreating side, a single lap configuration was studied. Tensile tests were conducted to evaluate the mechanical resistance and the surface fracture after a preliminary investigation consisting of morphological and microstructural analyses and microhardness measurements, with the goal of considering the possibility of replacing the typical joining processes, such as traditional welding or explosion welding, with friction stir welding. The testing showed that the joint produced on the advancing side performed better (+23.5% of the maximum load) than the joint made on the retreating side.
The Microstructural Degradation of Ni-Based Superalloys with Segregation under the Super-Gravity Condition
Authors: Yang Guo , Zhou Hui , Li Xueqiao , Wang Wenshuai , Long Haibo , Mao Shengcheng , Zhang Ze , Han Xiaodong
Published: February 2023

The Ni-based superalloy is used as the turbine blade, which is subject to the coupling effect of temperature and super-gravity during service. As the Ni-based superalloys are difficult to become homogenous after using the solid solution heat treatment, a study on morphology and composition distribution of Ni-base superalloys with segregation during microstructural degradation is necessary. This study investigates the microstructure of the ex-service turbine blade and cast samples subjected to the high-temperature centrifugal test. The difference in the size and shape factor of the γ′ phase decreased with the stress caused by the super-gravity condition, indicating a higher magnitude of homogenization degree. The higher stress will also promote the merge of the sub-grain boundaries, leading to a lower density and higher orientational deviation of the sub-grain boundaries.
Constituents of the Stem Bark of Trichilia monadelpha (Thonn.) J. J. De Wilde (Meliaceae) and Their Antibacterial and Antiplasmodial Activities
Authors: Djoumessi Arnauld Kenfack , Nono Raymond Ngansop , Neumann Beate , Stammler Hans-Georg , Bitchagno Gabin Thierry Mbahbou , Efange Noella Molisa , Nkenfou Celine Nguefeu , Ayong Lawrence , Lenta Bruno Ndjakou , Sewald Norbert , Nkeng-Efouet-Alango Pépin , Chouna Jean Rodolphe
Published: February 2023

The chemical investigation of the EtOH extract from the stem bark of Trichilia monadelpha (Thonn.) J. J. De Wilde afforded two new limonoids (1 and 2): 24-acetoxy-21,25-dihydroxy-21,23-epoxytirucall-7-en-3-one (1) and (6R)-1-O-deacetylkhayanolide E (2), together with eleven known compounds (3–13), including additional limonoids, flavonoids, triterpenoids, steroids, and fatty acid. Their structures were determined using 1D- and 2D-NMR experiments, ESI mass spectrometry, and single crystal X-ray diffraction analysis. The antibacterial and antiplasmodial activities of the extracts, sub-extracts, fractions, and some of the isolated compounds were evaluated in known pathogenic strains, including Staphylococcus aureus and Plasmodium falciparum. Fraction E (n-Hex/EtOAc 30:70, v/v) showed significant activity against S. aureus ATCC 25923 with a MIC value of 3.90 µg/mL, while one of its constituents (epicatechin (9)) exhibited significant activity with MIC values of 7.80 µg/mL. Interestingly, grandifotane A (6) (IC50 = 1.37 µM) and khayanolide D (5) (IC50 = 1.68 µM) were highly active against the chloroquine-sensitive/sulfadoxine-resistant plasmodium falciparum 3D7 strain, unlike their corresponding plant extract and fractions.
Probiotic Potential of Lactic Acid Bacterial Strains Isolated from Human Oral Microbiome
Authors: Atanasov Nikola , Evstatieva Yana , Nikolova Dilyana
Published: February 2023

Lactic acid bacteria are widely studied microorganisms and are one of the prevalent groups of bacteria in the oral cavity microbiome. This work aimed to isolate new lactic acid bacterial strains from the human oral cavity and evaluate their characteristics and probiotic potential. Twelve strains were isolated and identified as belonging to several genera in the family Lactobacillaceae. Screening for antimicrobial activity was held, where two of the strains showed antagonistic activity against Streptococcus mutans and most of the strains expressed inhibition against Escherichia coli, Bacillus subtilis, and Bacillus cereus. The ability of the studied strains to autoaggregate and bind to mucin was assessed, showing autoaggregative properties and mucin binding at 5 logs CFU/mL. The survival ability in simulated oral and gastrointestinal conditions and growth dynamics with different gastrointestinal stress factors was studied. Most of the strains showed a good growth potential in the presence of oral and gastrointestinal stress factors. All tested strains exhibited high survival rates in the simulated oral environment, thus having the potential for colonizing the oral cavity and their beneficial properties to be applied. These results are a good basis for continuing the research into these strains so they can be included in new functional products for oral health.
Lactic Bacteria with Plant-Growth-Promoting Properties in Potato
Authors: Panetto Lilian Dutra , Doria Joyce , Santos Carlos Henrique Barbosa , Frezarin Edvan Teciano , Sales Luziane Ramos , de Andrade Luana Alves , Rigobelo Everlon Cid
Published: February 2023

This study aimed to evaluate the abilities of three bacteria, Bacillus cereus, Succinovibrio dextrinosolvens, and Lactobacillus acidophilus, to fix nitrogen, solubilize phosphorus, and produce cellulosic and amylolytic enzymes. Then, these bacteria were evaluated in potato plants under field conditions. The bacterium B. cereus showed the ability to synthesize amylase, indole acetic acid (IAA) production of 9.08 μg mL−1, phosphorus solubilization of 14.93 mg P L−1, and nitrogen fixation of 0.7 mg of nitrogen L−1. S. dextrinosolvens showed the ability to synthesize siderophores and amylase, IAA production of 10.25 μg mL−1, phosphorus solubilization of 41.38 mg P L−1, and nitrogen fixation of 0.42 mg N L−1. L. acidophilus showed the ability to synthesize siderophores, IAA production of 7.25 μg mL−1, phosphorus solubilization of 5.58 mg P L−1, and nitrogen fixation of 0.5 mg N L−1. Some plant parameters were increased as shoot dry matter by B. cereus, and the mixture of bacteria increased shoot and root dry matter and increased phosphorus from the root. More studies are needed to deepen the understanding of the potential of these bacteria; however, B. cereus showed great potential to be used as a plant growth promoter in potato crops in the future.
A Healthy Vaginal Microbiota Remains Stable during Oral Probiotic Supplementation: A Randomised Controlled Trial
Authors: Lyra Anna , Ala-Jaakkola Reeta , Yeung Nicolas , Datta Neeta , Evans Kara , Hibberd Ashley , Lehtinen Markus J. , Forssten Sofia D. , Ibarra Alvin , Pesonen Tommi , Junnila Jouni , Ouwehand Arthur C. , Baranowski Keith , Maukonen Johanna , Crawford Gordon , Lehtoranta Liisa
Published: February 2023

The primary objective of this randomised, placebo-controlled, triple-blind study was to assess whether orally consumed Lactobacillus acidophilus La-14 (La-14) and Lacticaseibacillus rhamnosus HN001 (HN001) colonise a healthy human vagina. Furthermore, potential effects on vaginal microbiota and immune markers were explored. Fifty women devoid of vaginal complaints (Nugent score 0–3 and vaginal pH ≤ 4.5) were randomised into a 2-week intervention with either La-14 and HN001 as the verum product or a comparable placebo. Vaginal swab samples were collected at baseline, after one and two weeks of intervention, and after a one-week follow-up, for assessing colonisation of the supplemented lactobacilli, vaginal microbiota, and six specific immune markers. Colonisation of L. acidophilus and L. rhamnosus was not observed above the assay detection limit (5.29 and 5.11 log 10 genomes/swab for L. acidophilus and L. rhamnosus, respectively). Vaginal microbiotas remained stable and predominated by lactobacilli throughout the intervention, and vaginal pH remained optimal (at least 90% of participants in both groups had pH 4.0 or 4.5 throughout the study). Immune markers elafin and human β-defensin 3 (HBD-3) were significantly decreased in the verum group (p = 0.022 and p = 0.028, respectively) but did not correlate with any microbiota changes. Adverse events raised no safety concerns, and no undesired changes in the vaginal microbiota or immune markers were detected.
Depositional and Diagenetic Controls on Reservoir Quality of Neogene Surma Group from Srikail Gas Field, Bengal Basin, Bangladesh
Authors: Akter Maimuna , Rahman M. Julleh Jalalur , Ma Ming , Hossain Delwar , Khanam Farida
Published: February 2023

The development of an effective and profitable exploration and production depends heavily on the quality of the reservoir. The primary goal of this study was to evaluate the reservoir quality of the Neogene Surma Group at the Srikail Gas Field, which is situated in the western part of the eastern folded belt of the Bengal Basin, Bangladesh. Wire-line logs, core analysis, petrography, X-Ray diffraction (XRD) and a scanning electron microscope (SEM) were used to understand the depositional and diagenetic controls of the quality of the reservoir. The Surma Group of the Srikail Gas Field was deposited in a delta system with a dominant influence of tide. The subarkosic to sublitharenitic Neogene Surma Group sandstones have primary porosities ranging from 0% to 25.8%, with an average of 21.5%, and the secondary porosity is approximately 7%. The range of log porosity ranges from 15% to 22.2%, while log permeability and core permeability vary from 3.01 to 54.09 mD and 0.1 to 76 mD, respectively. The primary porosity had been destroyed mainly by mechanical and ductile grain compaction. Most of the clay minerals (illite/illite-smectite, chlorite and kaolinite) in sandstone occur as grain coatings, grain lining (rim) and a few occur as pore-filling. This study reveals that the reservoir quality is predominantly controlled by the depositional environment (sediment texture and facies, ductile grain supply, clay content), and diagenetic process (mainly mechanical and ductile grain compaction followed by clay cement). The information gathered from this research will be useful for future petroleum production and for enhancing predictability in order to find new prospects.
Headspace Solid Phase Micro-Extraction of Volatile Constituents Produced from Saudi Ruta chalepensis and Molecular Docking Study of Potential Antioxidant Activity
Authors: Aati Hanan Y. , Attia Hala , Babtin Razan , Al-Qahtani Najla , Wanner Juergen
Published: February 2023

Ruta chalepensis L., commonly known as Shazab in Saudi Arabia, is one of the famous culinary plants belonging to the Rutaceae family. It is commonly used in ethnomedicine in treating numerous diseases. This study was performed to characterize the essential oil isolated from Saudi species using a relatively new advanced headspace solid-phase microextraction technique. Following that, the antioxidant activity of the extracted oil was assessed using in vitro techniques such as the DPPH and nitric oxide scavenging tests, as well as the reducing power FRAP study and the molecular docking tool. The essential oil yield of the dried plant was 0.83% (v/w). Gas chromatography joined with a mass spectrometer was used to determine the chemical composition of the pale-yellow essential oil. Sixty-eight constituents were detected, representing 97.70% of the total oil content. The major constituents were aliphatic ketones dominated by 2-undecanone (37.30%) and 2-nonanone (20.00%), with minor constituents of mono and sesquiterpenoids chemical classes. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is one of the major causes of many contemporary diseases due to its ability to create a reactive oxygen species (ROS). Thus, molecular docking was used to confirm that some oil phytoconstituents have good docking scores compared to the standard antioxidant drug (Vitamin C), indicating great binding compatibility between the (NADPH) oxidase receptor site and the ligand. In conclusion, our findings suggest that the oil could be used safely and as a cost-effective remedy in treating various modern diseases caused by free radical formation.
Convenient Solid-Phase Attachment of Small-Molecule Ligands to Oligonucleotides via a Biodegradable Acid-Labile P-N-Bond
Authors: Kropacheva Nadezhda O. , Golyshkin Arseniy A. , Vorobyeva Mariya A. , Meschaninova Mariya I.
Published: February 2023

One of the key problems in the design of therapeutic and diagnostic oligonucleotides is the attachment of small-molecule ligands for targeted deliveries in such a manner that provides the controlled release of the oligonucleotide at a certain moment. Here, we propose a novel, convenient approach for attaching ligands to the 5′-end of the oligonucleotide via biodegradable, acid-labile phosphoramide linkage. The method includes the activation of the 5′-terminal phosphate of the fully protected, support-bound oligonucleotide, followed by interaction with a ligand bearing the primary amino group. This technique is simple to perform, allows for forcing the reaction to completion by adding excess soluble reactant, eliminates the problem of the limited solubility of reagents, and affords the possibility of using different solvents, including water/organic media. We demonstrated the advantages of this approach by synthesizing and characterizing a wide variety of oligonucleotide 5′-conjugates with different ligands, such as cholesterol, aliphatic oleylamine, and p-anisic acid. The developed method suits different types of oligonucleotides (deoxyribo-, 2′-O-methylribo-, ribo-, and others).
Chiral 8-Amino-5,6,7,8-tetrahydroquinoline Derivatives in Metal Catalysts for the Asymmetric Transfer Hydrogenation of 1-Aryl Substituted-3,4-dihydroisoquinolines as Alkaloids Precursors
Authors: Facchetti Giorgio , Neva Francesca , Coffetti Giulia , Rimoldi Isabella
Published: February 2023

Chiral diamines based on an 8-amino-5,6,7,8-tetrahydroquinoline backbone, known as CAMPY (L1), or the 2-methyl substituted analogue Me-CAMPY (L2) were employed as novel ligands in Cp* metal complexes for the ATH of a series of substituted dihydroisoquinolines (DHIQs), known for being key intermediates in the synthesis of biologically active alkaloids. Different metal-based complexes were evaluated in this kind of reaction, rhodium catalysts, C3 and C4, proving most effective both in terms of reactivity and enantioselectivity. Although modest enantiomeric excess values were obtained (up to 69% ee in the case of substrate I), a satisfactory quantitative conversion was successfully fulfilled even in the case of the most demanding hindered substrates when La(OTf)3 was used as beneficial additive, opening up the possibility for a rational design of novel chiral catalysts alternatives to the Noyori-Ikariya (arene)Ru(II)/TsDPEN catalyst.
Quantification of Enhydrin and Uvedalin in the Ethanolic Extract of Smallanthus sonchifolius Leaves Validated by the HPLC Method
Authors: Tamhid Hady Anshory , Hertiani Triana , Murti Yosi Bayu , Murwanti Retno
Published: February 2023

Yacon leaf (Smallanthus sonchifolius, Asteraceae) ethanolic extracts are widely used in herbal medicine preparation for diabetes. They contain two sesquiterpene lactones (enhydrin (1) and uvedalin (2)) as major bioactive compounds. To provide a suitable method of analysis for the extract’s quality control, we developed and validated a simultaneous HPLC-UV method using the compounds as markers. Compounds 1 and 2 were isolated using a freeze crystallization technique followed by a preparative HPLC. Spectrometry data for 1 and 2 were determined and compared to the literature. Chromatographic separation was carried out for 30 min with a mobile phase that used 60% water and 40% acetonitrile and a C18 column (250 × 4.6 mm, 5 µm) as the stationary phase. The flow was set to 1 mL min−1 and detection was conducted at 210 nm. The validation method was conducted according to the ICH guidelines, which included linearity, precision, accuracy, LOD, and LOQ. The calibration curve of both compounds was linear (R 2 > 0.9999), with the limit of detection and quantification as follows, respectively, 0.52 and 1.57 µg/mL for 1, and 0.144 and 0.436 µg/mL for 2. The percentages of recovery and repeatability (%RSD) were, 101.46 and 0.30% for 1, and 97.68 and 0.08% for 2, respectively. The 1 and 2 were 1.67 and 0.88% in the Ykal extract, and 1.26 and 0.56% in the Ycin extract, respectively. The method was found to be linear, precise, accurate, and suitable to be applied for control quality analyses of yacon leaf extract.
Profiles of Volatile Compounds from Seven New Hybrid Families Obtained by Crossings on Noir de Bourgogne Cultivar and Other Blackcurrant Varieties
Authors: Pagès-Hélary Sandy , Nars-Chasseray Marine , Dujourdy Laurence , Cayot Nathalie
Published: February 2023

Berries of blackcurrant (Ribes nigrum L.) are popular for their strong and complex aroma and their benefits for health. In Burgundy (France), the most famous blackcurrant cultivar is the “Noir de Bourgogne”. A blackcurrant breeding program was conducted to obtain new varieties, more resistant to infections and climate changes. The cultivar “Noir de Bourgogne” was crossed with seven other varieties in order to create a hybrid with good agronomic properties and organoleptic properties close to the ones of “Noir de Bourgogne”. Several hybrids were created, and their aromatic profiles studied. Berries of eight cultivars, among which Noir de Bourgogne and hybrids resulting from crossings, were harvested during the summer of 2020. Volatile compounds of berries were analyzed by HS-SPME-GC-MS, and principal component analysis (PCA) was used as the most useful chemometric technique. The profiles in volatile compounds of hybrids were either different from those of the two parental varieties or close to that of varieties other than Bourgogne black. In all cases, the overall aroma strength of the hybrid did not equal that of the Noir de Bourgogne cultivar.
Ag-Decorated Vertically Aligned ZnO Nanorods for Non-Enzymatic Glucose Sensor Applications
Authors: Lin Yu-Hsuan , Sivakumar Chandrasekar , Balraj Babu , Murugesan Gowtham , Nagarajan Senthil Kumar , Ho Mon-Shu
Published: February 2023

The non-enzymatic glucose sensing response of pure and Ag-decorated vertically aligned ZnO nanorods grown on Si substrates was investigated. The simple low-temperature hydrothermal method was employed to synthesize the ZnO NRs on the Si substrates, and then Ag decoration was achieved by sputtering. The crystal structure and surface morphologies were characterized by X-ray diffraction, field-emission scanning electron microscopy (FESEM), and transmission electron microscopy (TEM). The Ag incorporation on the ZnO NR surfaces was confirmed using EDS mapping and spectra. Furthermore, the chemical states, the variation in oxygen vacancies, and the surface modifications of Ag@ZnO were investigated by XPS analysis. Both the glucose/ZnO/Si and glucose/Ag@ZnO/Si device structures were investigated for their non-enzymatic glucose sensing performances with different glucose concentrations. Based on EIS measurements and amperometric analysis, the Ag@ZnO-NR-based glucose sensor device exhibited a better sensing ability with excellent stability over time than pure ZnO NRs. The Ag@ZnO NR glucose sensor device recorded 2792 µA/(mM·cm2) sensitivity with a lowest detection limit of 1.29 µM.
Effects of Whey Protein Supplement on 4-Week Resistance Exercise-Induced Improvements in Muscle Mass and Isokinetic Muscular Function under Dietary Control
Authors: Kim Chae-Been , Park Jong-Hoon , Park Hyoung-Su , Kim Hye-Jin , Park Jung-Jun
Published: February 2023

(1) The purpose of this study was to investigate the effect of whey protein supplementation under dietary control on improvements in muscle mass and function following resistance exercise training. (2) Thirty-two men were randomly assigned to a whey protein supplementation group taking whey protein isolate (PSG, n = 17) and a placebo group (CON, n = 15). Participants were provided with three meals per day corresponding to the estimated individual daily energy intake. The supervised resistance exercise program was conducted 60 min per day, six days per week, for four weeks. (3) Post-intervention, there was a significant interaction between groups in terms of muscle mass increase (p = 0.033, η2 = 0.14), with a greater increase in the PSG. There were also significant interactions between the groups and increases in peak torque of the dominant knee flexors (p = 0.048, η2 = 0.12), dominant shoulder extensors, and non-dominant shoulder extensors (p = 0.028, η2 = 0.15; p = 0.015, η2 = 0.18), and the total work of the dominant knee and shoulder extensors (p = 0.012, η2 = 0.19; p = 0.013, η2 = 0.19), with greater increases in the PSG. (4) These results suggest that whey protein supplementation enhances resistance exercise-induced increase in muscle mass and overall muscular strength and endurance, independent of dietary influence.
Enhancing Efficacy of a Brief Obesity and Eating Disorder Prevention Program: Long-Term Results from an Experimental Therapeutics Trial
Authors: Stice Eric , Rohde Paul , Butryn Meghan L. , Desjardins Christopher , Shaw Heather
Published: February 2023

Objective: Test whether the efficacy of Project Health, an obesity/eating disorder prevention program, is improved by delivering it in single-sex groups and adding food response inhibition and attention training. Method: High-risk young adults (N = 261; M age = 19.3, 74% female) were randomized to (1) single-sex or (2) mixed-sex groups that completed food response inhibition and attention training or (3) single-sex or (4) mixed-sex groups that completed sham training with nonfood images in a 2 × 2 factorial design. Results: There was a significant sex-composition by training-type by time interaction; participants who completed single- or mixed-sex Project Health groups plus food response and attention training showed significant reductions in body fat over a 2-year follow-up, though this effect was more rapid and persistent in single-sex groups, whereas those who completed single- or mixed-sex Project Health groups plus sham training did not show body fat change. However, there were no differences in overweight/obesity onset over the follow-up. The manipulated factors did not affect eating disorder symptoms or eating disorder onset, but there was a significant reduction in symptoms across the conditions (within-condition d = −0.58), converging with prior evidence that Project Health produced larger reductions in symptoms (within-condition d = −0.48) than educational control participants. Average eating disorder onset over the 2-year follow-up (6.4%) was similar to that observed in Project Health in a past trial (4.5%). Conclusions: Given that Project Health significantly reduced future onset of overweight/obesity in a prior trial and the present trial found that body fat loss effects were significantly greater when implemented in single-sex groups and paired with food response and attention training, there might be value in broadly implementing this combined intervention.
Difficulties in Caring for the Older Adults: Perspective of Brazilian and Portuguese Caregivers
Authors: Santana Elaine , Mendes Felismina , Bernardo Joana , Silva Rosa , Melo Pedro , Lima Pollyanna , Oliveira Alessandra , Reis Luciana
Published: February 2023

This study aims to understand the difficulties in caring for the older adults with functional dependence from the perspective of Brazilian and Portuguese caregivers. This is a study based on the Theory of Social Representations, based on the Thematic Content Analysis proposed by Bardin, carried out with 21 informal caregivers of older adults in Brazil and 11 informal caregivers of older adults in Portugal. The instrument consisted of a questionnaire with sociodemographic data and data on health conditions along with an open interview with guiding questions on the theme of care. Data were analyzed using the Content Analysis technique proposed by Bardin, with the help of the QRS NVivo® Version 11 software (QSR International, Burlington, MA, USA). Three categories emerged from the speeches: “Caregiver burden”, “Caregiver support network” and “Older adults resistance”. The main difficulties mentioned by caregivers were associated with family articulation in meeting the needs of their older adults, whether due to the excessive demand of tasks, which results in overloading the caregiver, or the behaviors of the older adults themselves, or even the availability of a truly supportive and effective network.