bluuebunny/crossref_metadata_2025 huggingface dataset:
Computational model for fetal skeletal defects potentially linked to disruption of retinoic acid signaling
Authors: Pierro Jocylin D. , Ahir Bhavesh K. , Baker Nancy C. , Kleinstreuer Nicole C. , Xia Menghang , Knudsen Thomas B.
Published: September 2022

All-trans retinoic acid (ATRA) gradients determine skeletal patterning morphogenesis and can be disrupted by diverse genetic or environmental factors during pregnancy, leading to fetal skeleton defects. Adverse Outcome Pathway (AOP) frameworks for ATRA metabolism, signaling, and homeostasis allow for the development of new approach methods (NAMs) for predictive toxicology with less reliance on animal testing. Here, a data-driven model was constructed to identify chemicals associated with both ATRA pathway bioactivity and prenatal skeletal defects. The phenotype data was culled from ToxRefDB prenatal developmental toxicity studies and produced a list of 363 ToxRefDB chemicals with altered skeletal observations. Defects were classified regionally as cranial, post-cranial axial, appendicular, and other (unspecified) features based on ToxRefDB descriptors. To build a multivariate statistical model, high-throughput screening bioactivity data from >8,070 chemicals in ToxCast/Tox21 across 10 in vitro assays relevant to the retinoid signaling system were evaluated and compared to literature-based candidate reference chemicals in the dataset. There were 48 chemicals identified for effects on both in vivo skeletal defects and in vitro ATRA pathway targets for computational modeling. The list included 28 chemicals with prior evidence of skeletal defects linked to retinoid toxicity and 20 chemicals without prior evidence. The combination of thoracic cage defects and DR5 (direct repeats of 5 nucleotides for RAR/RXR transactivation) disruption was the most frequently occurring phenotypic and target disturbance, respectively. This data model provides valuable AOP elucidation and validates current mechanistic understanding. These findings also shed light on potential avenues for new mechanistic discoveries related to ATRA pathway disruption and associated skeletal dysmorphogenesis due to environmental exposures.
Cannabidiol prevents methamphetamine-induced neurotoxicity by modulating dopamine receptor D1-mediated calcium-dependent phosphorylation of methyl-CpG-binding protein 2
Authors: Shen Baoyu , Zhang Ruilin , Yang Genmeng , Peng Yanxia , Nie Qianyun , Yu Hao , Dong Wenjuan , Chen Bingzheng , Song Chunhui , Tian Yan , Qin Lixiang , Shu Junjie , Hong Shijun , Li Lihua
Published: September 2022

In the past decade, methamphetamine (METH) abuse has sharply increased in the United States, East Asia, and Southeast Asia. METH abuse not only leads to serious drug dependence, but also produces irreversible neurotoxicity. Currently, there are no approved pharmacotherapies for the treatment of METH use disorders. Cannabidiol (CBD), a major non-psychoactive (and non-addictive) cannabinoid from the cannabis plant, shows neuroprotective, antioxidative, and anti-inflammatory properties under METH exposure. At present, however, the mechanisms underlying these properties remain unclear, which continues to hinder research on its therapeutic potential. In the current study, computational simulations showed that CBD and METH may directly bind to the dopamine receptor D1 (DRD1) via two overlapping binding sites. Moreover, CBD may compete with METH for the PHE-313 binding site. We also found that METH robustly induced apoptosis with activation of the caspase-8/caspase-3 cascade in-vitro and in-vivo, while CBD pretreatment prevented these changes. Furthermore, METH increased the expression of DRD1, phosphorylation of Methyl-CpG-binding protein 2 (MeCP2) at serine 421 (Ser421), and level of intracellular Ca2+in-vitro and in-vivo, but these effects were blocked by CBD pretreatment. The DRD1 antagonist SCH23390 significantly prevented METH-induced apoptosis, MeCP2 phosphorylation, and Ca2+ overload in-vitro. In contrast, the DRD1 agonist SKF81297 markedly increased apoptosis, MeCP2 phosphorylation, and Ca2+ overload, which were blocked by CBD pretreatment in-vitro. These results indicate that CBD prevents METH-induced neurotoxicity by modulating DRD1-mediated phosphorylation of MeCP2 and Ca2+ signaling. This study suggests that CBD pretreatment may resist the effects of METH on DRD1 by competitive binding.
Comparative effectiveness of individualized longer and standardized shorter regimens in the treatment of multidrug resistant tuberculosis in a high burden country
Authors: Wahid Abdul , Ghafoor Abdul , Khan Abdul Wali , Al-Worafi Yaser Mohammed , Latif Abdullah , Shahwani Nisar Ahmed , Atif Muhammad , Saleem Fahad , Ahmad Nafees
Published: September 2022

Objective: To compare the effectiveness of second line injectables containing shorter (duration 9–12 months) and longer treatment regimens (LTR, duration ≥ 20 months) among multidrug-resistant tuberculosis (MDR-TB) patients with no documented resistance and history of treatment with any second-line anti-TB drug (SLD) for ≥ 1 month.

Methods: This was an observational cohort study of MDR-TB patients treated at eight PMDT units in Pakistan. Patients’ data from baseline until treatment outcomes were collected from Electronic Nominal Recording and Reporting System. The treatment outcomes of “cured” and “treatment completed” were grouped together as successful, whereas “death,” “treatment failure,” and “lost to follow-up” were collectively grouped as unsuccessful outcomes. Time to sputum culture conversion (SCC) was analyzed using the Kaplan–Meier method and the differences between groups were compared through the log-rank test. Multivariate Cox proportional hazards and binary logistic regression analyses were used to find predictors of time to SCC and unsuccessful treatment outcomes. A p-value < 0.05 was considered statistically significant.

Results: A total 701 eligible MDR-TB patients [313 treated with shorter treatment regimen (STR) and 388 treated with LTR at eight centres in Pakistan were evaluated]. Time to achieve SCC was significantly shorter in STR group [mean: 2.03 months, 95% confidence interval (CI):1.79–2.26] than in LTR group (mean: 2.69 months, 95% CI: 2.35–3.03) (p-value<0.001, Log-rank test). Treatment success was higher in STR (83.7%) than in LTR (73.2%) group (p-value <0.001) due to high cure (79.9% vs. 70.9%, p-value = 0.006) and low death (9.9% vs. 18.3%, p-value = 0.002) rates with STR. Treatment with STR emerged the only predictor of early SCC [adjusted Hazards ratio (aHR) = 0.815, p-value = 0.014], whereas, patient’s age of 41–60 (OR = 2.62, p-value<0.001) and >60 years (OR = 5.84, p-value<0.001), baseline body weight of 31–60 (OR = 0.36, p-value = 0.001) and >60 kg (OR = 0.23, p-value <0.001), and treatment with LTR (OR = 1.88, p-value = 0.001) had statistically significant association with unsuccessful treatment outcomes.

Conclusion: STR exhibited superior anti-microbial activity against MDR-TB. When compared LTR, treatment with STR resulted in significantly early SCC, high cure, and lower death rates among MDR-TB patients who had no documented resistance and history of treatment with any SLD ≥ 1 month.
Terahertz reconfigurable dielectric metasurface hybridized with vanadium dioxide for two-dimensional multichannel multiplexing
Authors: Wang Ling , Yang Yang , Gao Feng , Teng Shuhua , Tan Zhi-Guo , Zhang Xing , Lou Jun , Deng Li
Published: September 2022

The metasurface hybridized with vanadium dioxide (VO2) can be dynamically tuned, which has attracted enormous attention in recent years and orbital angular momentum (OAM) multiplexing based on metasurfaces has shown promising prospects in terahertz communications. However, existing research on VO2 metasurface focuses on the metallic metasurface. The dielectric VO2 metasurface used for OAM multiplexing is rarely reported to the present. This paper proposed a terahertz reconfigurable dielectric metasurface hybridized with VO2 for two-dimensional multichannel multiplexing combing with spatial and frequency domains. The metasurface works in both reflection and transmission modes and simultaneously the polarization control and operating frequency band regulation can be realized by switching the VO2 from the metallic state to the insulator state. For the reflective or transmissive metasurface, when 4×M-channel (M is a positive integer) off-axis plane waves are incident on the metasurface, the co-polarization reflected or cross-polarization transmitted waves are transformed into 4×M-channel orthogonal on-axis beams with topological or frequency orthogonality. A metasurface composed of 14 × 14 unit cells is designed for verification. The simulated result shows that two-dimensional 12-channel multiplexing combing with OAM and frequency by the designed metasurface can be realized on the reflection and transmission modes in two different frequency bands. The proposed metasurface has great potential in terahertz communications.
A glucose-insulin-glucagon coupled model of the isoglycemic intravenous glucose infusion experiment
Authors: Subramanian Vijaya , Bagger Jonatan I. , Holst Jens J. , Knop Filip K. , Vilsbøll Tina
Published: September 2022

Type 2 diabetes (T2D) is a pathophysiology that is characterized by insulin resistance, beta- and alpha-cell dysfunction. Mathematical models of various glucose challenge experiments have been developed to quantify the contribution of insulin and beta-cell dysfunction to the pathophysiology of T2D. There is a need for effective extended models that also capture the impact of alpha-cell dysregulation on T2D. In this paper a delay differential equation-based model is developed to describe the coupled glucose-insulin-glucagon dynamics in the isoglycemic intravenous glucose infusion (IIGI) experiment. As the glucose profile in IIGI is tailored to match that of a corresponding oral glucose tolerance test (OGTT), it provides a perfect method for studying hormone responses that are in the normal physiological domain and without the confounding effect of incretins and other gut mediated factors. The model was fit to IIGI data from individuals with and without T2D. Parameters related to glucagon action, suppression, and secretion as well as measures of insulin sensitivity, and glucose stimulated response were determined simultaneously. Significant impairment in glucose dependent glucagon suppression was observed in patients with T2D (duration of T2D: 8 (6–36) months) relative to weight matched control subjects (CS) without diabetes (k1 (mM)−1: 0.16 ± 0.015 (T2D, n = 7); 0.26 ± 0.047 (CS, n = 7)). Insulin action was significantly lower in patients with T2D (a1 (10 pM min)−1: 0.000084 ± 0.0000075 (T2D); 0.00052 ± 0.00015 (CS)) and the Hill coefficient in the equation for glucose dependent insulin response was found to be significantly different in T2D patients relative to CS (h: 1.4 ± 0.15; 1.9 ± 0.14). Trends in parameters with respect to fasting plasma glucose, HbA1c and 2-h glucose values are also presented. Significantly, a negative linear relationship is observed between the glucagon suppression parameter, k1, and the three markers for diabetes and is thus indicative of the role of glucagon in exacerbating the pathophysiology of diabetes (Spearman Rank Correlation: (n = 12; (−0.79, 0.002), (−0.73,.007), (−0.86,.0003)) respectively).
A Review of free fatty acid-induced cell signaling, angiopoietin-like protein 4, and skeletal muscle differentiation
Authors: Son Yura , Paton Chad M.
Published: September 2022

Postnatal skeletal muscle differentiation from quiescent satellite cells is a highly regulated process, although our understanding of the contribution of nutritional factors in myogenesis is limited. Free fatty acids (FFAs) are known to cause detrimental effects to differentiated skeletal muscle cells by increasing oxidative stress which leads to muscle wasting and insulin resistance in skeletal muscle. In addition, FFAs are thought to act as inhibitors of skeletal muscle differentiation. However, the precise molecular mechanisms underlying the effects of FFAs on skeletal muscle differentiation remains to be elucidated. There is a clear relationship between dietary FFAs and their ability to suppress myogenesis and we propose the hypothesis that the FFA-mediated increase in angiopoietin-like protein 4 (ANGPTL4) may play a role in the inhibition of differentiation. This review discusses the role of FFAs in skeletal muscle differentiation to-date and proposes potential mechanisms of FFA-induced ANGPTL4 mediated inhibition of skeletal muscle differentiation.
Analysis of glutathione-S-transferases from larvae of Galleria mellonella (Lepidoptera, Pyralidae) with potential alkaloid detoxification function
Authors: Venthur Herbert , Lizana Paula , Manosalva Loreto , Rojas Valentina , Godoy Ricardo , Rocha Adonis , Aguilera Iván , Palma-Millanao Rubén , Fajardo Victor , Quiroz Andrés , Mutis Ana
Published: September 2022

The greater wax moth, Galleria mellonella, is a global pest for beehives, doing damage in the larval stage. Although a significant number of studies have reported on larvae and adults, to date no effective pest control has been implemented. In this study, we tested larval resistance to alkaloids from Berberis microphylla, and the objective was to identify enzymes that participate in alkaloid detoxification through enzymatic assays, bioinformatics analysis and qRT-PCR. Findings suggest glutathione-S-transferases (GSTs), from an increased metabolic mechanism, are responsible for alkaloid detoxification rather than cytochrome P450 (CYP), carboxylesterases (CarE). A bioinformatics analysis from transcriptome data revealed 22 GSTs present in both G. mellonella larvae and adults. The qRT-PCR experiments corroborated the presence of the 22 GSTs in larvae, where GST8 and GST20 stood out with the highest expression after berberine treatment. Structural information around GST8 and GST20 suggests that GST8 could bind berberine stronger than GST20. These findings represent an important advance in the study of detoxification enzymes in G. mellonella, expanding the role of delta-class GSTs towards alkaloids. Likewise, GST inhibition by alkaloid analogs is proposed in the framework of integrated pest management strategies.
Why are bleeding trauma patients still dying? Towards a systems hypothesis of trauma
Authors: Dobson Geoffrey P. , Morris Jodie L. , Letson Hayley L.
Published: September 2022

Over the years, many explanations have been put forward to explain early and late deaths following hemorrhagic trauma. Most include single-event, sequential contributions from sympathetic hyperactivity, endotheliopathy, trauma-induced coagulopathy (TIC), hyperinflammation, immune dysfunction, ATP deficit and multiple organ failure (MOF). We view early and late deaths as a systems failure, not as a series of manifestations that occur over time. The traditional approach appears to be a by-product of last century’s highly reductionist, single-nodal thinking, which also extends to patient management, drug treatment and drug design. Current practices appear to focus more on alleviating symptoms rather than addressing the underlying problem. In this review, we discuss the importance of the system, and focus on the brain’s “privilege” status to control secondary injury processes. Loss of status from blood brain barrier damage may be responsible for poor outcomes. We present a unified Systems Hypothesis Of Trauma (SHOT) which involves: 1) CNS-cardiovascular coupling, 2) Endothelial-glycocalyx health, and 3) Mitochondrial integrity. If central control of cardiovascular coupling is maintained, we hypothesize that the endothelium will be protected, mitochondrial energetics will be maintained, and immune dysregulation, inflammation, TIC and MOF will be minimized. Another overlooked contributor to early and late deaths following hemorrhagic trauma is from the trauma of emergent surgery itself. This adds further stress to central control of secondary injury processes. New point-of-care drug therapies are required to switch the body’s genomic and proteomic programs from an injury phenotype to a survival phenotype. Currently, no drug therapy exists that targets the whole system following major trauma.
Production of recombinant human xCT (SLC7A11) and reconstitution in proteoliposomes for functional studies
Authors: Galluccio Michele , Scalise Mariafrancesca , Pappacoda Gilda , Scarpelli Martina , Bonanomi Marcella , Gaglio Daniela , Indiveri Cesare
Published: September 2022

The plasma membrane transporter xCT belongs to the SLC7 family and has the physiological role of mediating the exchange of glutamate and cystine across the cell plasma membrane, being crucial for redox control. The xCT protein forms a heterodimer with the ancillary protein CD98. Over the years, xCT became a hot pharmacological target due to the documented over-expression in virtually all human cancers, which rely on cystine availability for their progression. Notwithstanding, several unknown aspects of xCT biology still exist that require a suitable single protein experimental model, to be addressed. To this aim, the recombinant host Escherichia coli has been exploited to over-express the human isoform of xCT. In this widely used and low-cost system, the optimization for growth and protein production has been achieved by acting on the metabolic needs of the bacterial strains. Then, the His-tagged protein has been purified by Ni2+-chelating chromatography and reconstituted in proteoliposomes for transport activity assays. The expressed protein was in a folded/active state allowing functional and kinetic characterization. Interestingly, the features of the recombinant protein meet those of the native one extracted from intact cells, further confirming the suitability of E. coli as a host for the expression of human proteins. This study opens perspectives for elucidating other molecular aspects of xCT, as well as for studying the interaction with endogenous and exogenous compounds, relevant to human health.
Convergence without divergence in North American red-flowering Silene
Authors: Berardi Andrea E. , Betancourt Morejón Ana C. , Hopkins Robin
Published: September 2022

Combinations of correlated floral traits have arisen repeatedly across angiosperms through convergent evolution in response to pollinator selection to optimize reproduction. While some plant groups exhibit very distinct combinations of traits adapted to specific pollinators (so-called pollination syndromes), others do not. Determining how floral traits diverge across clades and whether floral traits show predictable correlations in diverse groups of flowering plants is key to determining the extent to which pollinator-mediated selection drives diversification. The North American Silene section Physolychnis is an ideal group to investigate patterns of floral evolution because it is characterized by the evolution of novel red floral color, extensive floral morphological variation, polyploidy, and exposure to a novel group of pollinators (hummingbirds). We test for correlated patterns of trait evolution that would be consistent with convergent responses to selection in the key floral traits of color and morphology. We also consider both the role of phylogenic distance and geographic overlap in explaining patterns of floral trait variation. Inconsistent with phenotypically divergent pollination syndromes, we find very little clustering of North American Silene into distinct floral morphospace. We also find little evidence that phylogenetic history or geographic overlap explains patterns of floral diversity in this group. White- and pink-flowering species show extensive phenotypic diversity but are entirely overlapping in morphological variation. However, red-flowering species have much less phenotypic disparity and cluster tightly in floral morphospace. We find that red-flowering species have evolved floral traits that align with a traditional hummingbird syndrome, but that these trait values overlap with several white and pink species as well. Our findings support the hypothesis that convergent evolution does not always proceed through comparative phenotypic divergence, but possibly through sorting of standing ancestral variation.
Effects of land use change on population survival of three wild rice species in China since 2001
Authors: Chen Hao , Dong Shanshan , He Zhizhou , Chen Yuhong , Tian Defeng , Liu Yan , Wang Yuguo , Zhang Wenju , Li Linfeng , Yang Ji , Song Zhiping
Published: September 2022

Land use change stemming from human activities, particularly cropland expansion, heavily threatens the survival of crop wild relatives that usually occur nearby or scatter in farming systems. Understanding the impacts of land use change on wild populations is critical in forming the conservation decision-making of wild relatives. Based on the investigations on the population survival of three wild rice species (Oryza rufipogon, O. officinalis, and O. granulata) in China over the past 40 years (1978–2019), the effect of land use change during the past 20 years (2001–2019) on the natural populations of the three species was examined using the land use type data of satellite-based Earth observations (data from GlobCover). From 1978 to 2019, the number of populations (distribution sites) of the three wild rice species had decreased by 65–87%, mainly because of the habitat destruction or disappearance caused by human-induced land use change. The three wild rice species display different habitat preferences, resulting in specific land use types surrounding their populations. In the recent 20 years, although the surrounding community composition of the wild rice population has been relatively stable, the surrounding vegetation cover area of the survived populations was significantly more extensive than that of the extinct ones (p < 0.05). These findings suggest that habitat vegetation plays a “biological barrier” role in the survival of wild populations through resisting or mitigating the disturbing impact of land use change on wild populations. This study provides not only direct guidelines for the conservation of wild rice but also new insights into the mechanisms underlying the influence of land use change on wild populations.
Effects of rare-earth light conversion film on the growth and fruit quality of sweet pepper in a solar greenhouse
Authors: Gao Yaxin , Li Gongfeng , Cai Bingbing , Zhang Ziming , Li Ning , Liu Yike , Li Qingyun
Published: September 2022

Light is an important environmental factor influencing plant growth and development. However, artificial light supplement is difficult to spread for its high energy consumption. In recent years, rare-earth light conversion film (RPO) covering is being focused on to be a new technology to study the mechanism of light affecting plant growth and development. Compared with the polyolefin film (PO), the RPO film advanced the temperature and light environment inside the greenhouse. Ultimately, improved growth and higher yield were detected because of a higher photosynthesis, Rubisco activity and Rubisco small subunit transcription. Compared with that in the greenhouse with polyolefin film, the plant height, stem diameter and internode length of sweet pepper treated with RPO increased by 11.05, 16.96 and 25.27%, respectively. In addition, Gibberellic acid 3 (GA3), Indole-3-acetic acid (IAA), Zeatin Riboside contents were increased by 11.95, 2.84 and 16.19%, respectively, compared with that with PO film. The fruit quality was improved, and the contents of ascorbic acid (Vc), soluble protein and soluble sugar were significantly higher than those of PO film, respectively, increased by 14.29, 47.10 and 67.69%. On the basis of improved fruit quality, the yield of RPO treatment increased by 20.34% compared with PO film. This study introduces an effective and low-energy method to study the mechanism and advancing plant growth in fruit vegetables production.
Outstanding women psychologists mainly from Europe – What helped and what limited them in their scientific careers? Guidelines for gender equity programs in academia
Authors: Pastwa-Wojciechowska Beata , Chybicka Aneta
Published: September 2022

The manuscript is based on a series of structured interviews with female scientists from around the world who have made significant contributions to psychology and have an impact on their cultural areas. The authors interviewed female scientists and researchers from a similar age group, but from different regions of the world, to capture the factors influencing careers of interlocutors from a similar period and enabling cultural inference. Both the universal and the cultural barriers faced by female scientists/researchers in career development and the factors that have contributed to success in psychology are discussed. Universal and cultural factors served in this manuscript as a guideline for gender equality programs in academia to overcome gender stereotypes, support early career development, support women in reaching leadership positions, and enhance women’s visibility.
Short-term efficacy of music therapy combined with α binaural beat therapy in disorders of consciousness
Authors: Liu Zi-Bo , Liu Yan-Song , Zhao Long , Li Man-Yu , Liu Chun-Hui , Zhang Chun-Xia , Li Hong-Ling
Published: September 2022

Objective

To investigate the short-term effect of music therapy combined with binaural frequency difference therapy on patients with consciousness disorder.

Materials and methods

Ninety patients with definite diagnosis of disorders of consciousness (DOC) were selected. These patients were randomly divided into control group, experiment 1 group and experiment 2 group, with 30 patients in each group. The control group was treated with routine clinical treatment and rehabilitation. In experiment 1 group, music therapy was added to the control group. In experimental group 2, music therapy combined with binaural α frequency difference therapy was added to the control group. All patients were assessed before and after 30 treatments. The assessment items included Glasgow Coma Scale (GCS), Coma Recovery Scale revised (CRS-R), electroencephalogram (EEG), upper somatosensory evoked potential (USEP), and brainstem auditory evoked potential (BAEP).

Results

Before treatment, there were no significant differences in GCS score, CRS-R score, USEP, BAEP, and EEG scores among the three groups (P > 0.05). After 30 times of treatment, GCS score, CRS-R score, USEP, BAEP, and EEG scores in 3 groups were significantly higher than those before treatment (P < 0.05), and experimental group 2 >experimental group 1 >control group (P < 0.05). And the consciousness rate of experimental group 2 was better than experimental group 1, experimental group 1 was better than the control group and the difference was statistically significant (P < 0.05).

Conclusion

Music therapy combined with binaural α frequency difference therapy is more effective in stimulating DOC patients.
Research on the correlation between component elements and psychological perception of the spatial form of underground commercial street corners based on virtual reality technology
Authors: Sun Liang , Fang Tianyu , Sun Yan , Wang Bo , Shao Zebiao
Published: September 2022

The corner space, an important area of underground commercial streets, not only converts space functions but also exerts a great impact on the space atmosphere by transforming the environmental quality of the commercial street space. Based on corner space investigations of several underground commercial streets in China, this paper constructs a realistic scene model using virtual reality technology and screens. This paper classifies the corner space elements of underground commercial streets through preliminary experiments. Based on the conclusions, different morphological models of the corner space were constructed by orthogonal experiments and virtual reality technology combined with psychology. A semantic differential was used to quantitatively evaluate and analyze the spatial experience cognition of the subjects. This enabled an analysis of the of underground commercial street corners’ relationship to the component elements and the visitors’ psychological perception of their spatial form.
Authentic leadership and flourishing: Do trust in the organization and organizational support matter during times of uncertainty?
Authors: Kleynhans Deon J. , Heyns Marita M. , Stander Marius W.
Published: September 2022

Orientation: This study investigated the influence of authentic leadership on employee flourishing while considering the potential mediating effect of trust in the organization and organizational support as underlying mechanisms in an uncertain setting.

Research purpose: To examine the relationship between authentic leadership and employee flourishing by evaluating the indirect effect of organizational support and trust in the organization as potential mediators.

Motivation for the study: An authentic leadership approach, organizational support, and trust in the organization may influence the flourishing of employees in uncertain times. Increasing the comprehension of the possible interaction effect of organizational support and trust in the organization in the relationship between authentic leadership and employee flourishing may improve individual and organizational efficiency.

Research approach/design and method: A quantitative, cross-sectional survey design was applied in this study. The sample comprised 314 employees in a noteworthy South African steel manufacturing entity. The Authentic Leadership Inventory, Workplace Trust Survey, Flourishing-at-Work Scale, and the Job Demands-Resources Scale were administered.

Main findings: The findings of this study suggest that authentic leadership was a significant predictor of employee flourishing through organizational support and trust in the organization.

Practical/managerial implications: This research illuminates the potential value-adding contribution of an authentic leadership style in promoting a trust-filled relationship between team members and their organization and the support they experience from their employer. Despite the prevailing precarious context, working under the mentioned conditions might result in the increased flourishing of employees.

Contribution/value-add: The analyses of the mentioned relationships might assist businesses in optimizing the resources required to improve employee and organizational performance. Additionally, the exploration of organizational support in conjunction with organizational trust raises our understanding of the possible influence these elements can have in enhancing employee flourishing in the workplace.
The roles of social status information in irony comprehension: An eye-tracking study
Authors: Wu Zixuan , Wang Yuxia
Published: September 2022

The literature on irony processing mainly focused on contextual effect, leaving other factors (such as social factors) untouched. The current study investigated how social status information affected the online comprehension of irony. As irony might be more damaging when a speaker uses it to a superordinate than the other way around, it is assumed that greater processing efforts would be observed in the former case. Using an eye-movement sentence reading paradigm, we recruited 36 native Mandarin speakers and examined the role of social status information and literality (i.e., literal and irony) in their irony interpretation. Our results showed ironic statements were more effortful to process than literal ones, reporting an early and consistent effect on the target regions. The social status effect followed the literality effect, with more difficulty in processing ironic statements that targeted the superordinate than the subordinate; such an effect of social status was missing with literal statements. Besides, an individual’s social skill appeared to affect the perception of status information in ironic statements, as the socially skillful readers needed more time than the socially unskillful to process irony targeting a subordinate in the second half of the experiment in the critical region. Our study suggests that irony processing might be further discussed in terms of the relative predictability of linguistic, social, and individual variabilities.
Influencing factors of psychological stress under the mixed teaching mode based on SPOC+PBL
Authors: Shen Guorong , An Yide
Published: September 2022

In the teaching process, teachers and students are under different psychological pressures. Especially under the influence of the epidemic, many teaching modes have been transformed from traditional classroom teaching to online teaching. Under the new teaching mode, especially the promotion and application of the blended teaching mode, classroom teaching is facing new challenges, and both students and teachers are facing new psychological pressures. If the psychological pressure is not resolved, the classroom effect will be even worse. Therefore, the purpose of this paper is to analyze the source of the influencing factors of psychological stress under the new teaching mode, which is a rarely involved direction in the teaching field, and is of great significance for the promotion and application of the new teaching mode. Aiming at the blended teaching model, this paper focuses on the SPOC+PBL blended teaching model, designs a new teaching model of “English-Chinese translation and interpretation”, and studies the sources of pressure under the new model. Aiming at the influencing factors of psychological pressure, a psychological pressure model is established to explore the influence of six factors on psychological pressure. The experimental results of this paper show that the factor loadings of all variables are greater than 0.5, indicating that “self-esteem strength”, “self-efficacy”, “help-seeking experience”, “psychological counseling cognitive bias”, “counseling object positioning bias” and “psychological counseling” “Resources” and “availability” have a greater impact on psychological stress.
Learning English vocabulary from word cards: A research synthesis
Authors: Lei Yuanying , Reynolds Barry Lee
Published: September 2022

Researchers' interest in the learning of vocabulary from word cards has grown alongside the increasing number of studies published on this topic. While meta-analyses or systematic reviews have been previously performed, the types of word cards investigated, and the number of word card studies analyzed were limited. To address these issues, a research synthesis was conducted to provide an inclusive and comprehensive picture of how the use of word cards by learners results in vocabulary learning. A search of the Web of Science and Scopus databases resulted in 803 potential studies, of which 32 aligned with the inclusion criteria. Coding of these studies based on an extensive coding scheme found most studies assessed receptive vocabulary knowledge more often than productive vocabulary knowledge, and knowledge of vocabulary form and meaning were assessed more often than knowledge of vocabulary use. Results of effect size plots showed that more of the reviewed studies showed larger effects for the use of paper word cards than digital word cards, and for the use of ready-made word cards than self-constructed word cards. Results also indicated more studies showed larger effects for using word cards in an intentional learning condition compared with an incidental learning condition, and for using word cards in a massed learning condition compared with a spaced learning condition. Although a correlation was found between time spent using word cards and vocabulary learning outcomes, this correlation was not statistically significant. Learners that were more proficient in English learned more words from using word cards than those less proficient. These results suggest that future researchers should report learner proficiency, adopt reliable tests to assess vocabulary learning outcomes, compare the effectiveness of ready-made word cards and self-constructed word cards, and investigate the learning of different aspects of word knowledge. Teachers should provide learners guidance in how to use word cards and target word selection for self-construction of word cards. In addition, teachers should encourage learners to create word cards for incidentally encountered unknown words and use massed learning when initially working with these new words before using spaced learning for later retrieval practice.
Antecedents of attitude and their impact on behavioral intention in the staycation context
Authors: Zhang Yating , Shen Huawen , Xu Jiajia , Qian Stella Fang
Published: September 2022

The year 2020 and 2021 have been decimated by the pandemic, leading to outbound vacations largely scrapped. Staycation, a typical domestic journal, has then been adopted by those who are tired of self-isolation for so long. This study aims to explore and assess the drivers exerting impact on attitude of tourists toward staycation and the interrelationship among the research constructs is also examined. A quantitative analysis is employed for evaluating the roles of reduced risk perception, benign envy, and perceived benefits as they exert the effect on attitude toward staycation. An online questionnaire survey was used, and a total of 213 samples were collected from target respondents in Hong Kong, which were still under lockdown at the time of the study. The results of the study showed that reduced risk perception, benign envy as well as perceived benefits will influence tourists’ attitude toward staycation. The managerial and theoretical implications of the results are discussed based on the significant relationships identified in the study.
Development of a balanced instrument to measure global health-related quality of life: The 13-MD
Authors: Touré Moustapha , Lesage Alain , Poder Thomas G.
Published: September 2022

Generic instruments are of interest in measuring global health-related quality of life (GHRQoL). Their applicability to all patients, regardless of their health profile, allows program comparisons of whether the patients share the same disease or not. In this setting, quality-adjusted life-year (QALY) instruments must consider GHRQoL to allow the best programs to emerge for more efficiency in health resource utilization. However, many differences may be perceived among the existing generic instruments relative to their composition, where dimensions related to physical aspects of health are generally depicted more often than dimensions related to mental or social aspects. The objective of this study was to develop a generic instrument that would be complete in its covered meta-dimensions and reflect, in a balanced way, the important aspects of GHRQoL. To this end, a Delphi procedure was held in four rounds, gathering 18 participants, including seven patients, six caregivers, and five citizens. The structure of the instrument derived from the Delphi procedure was submitted to psychometric tests using data from an online survey involving the general population of Quebec, Canada (n = 2,273). The resulting questionnaire, the 13-MD, showed satisfying psychometric properties. It comprises 33 items or dimensions, with five to seven levels each. The 13-MD reflects, in a balanced form, the essential aspects of GHRQoL by including five meta-dimensions for physical health, four meta-dimensions for mental health, three meta-dimensions for social health, and one meta-dimension for sexuality and intimacy. The next step will involve the development of a value set for the 13-MD to allow QALY calculation.
Effects of adjunctive pimavanserin and current antipsychotic treatment on QT interval prolongation in patients with schizophrenia
Authors: Bugarski-Kirola Dragana , Nunez Rene , Odetalla Ramzey , Liu I-Yuan , Turner Mary Ellen
Published: September 2022

Background

Pimavanserin prolongs the QT interval, with mean increases in corrected QT (QTc) of 5–8 ms, and is currently being investigated for the treatment of negative symptoms of schizophrenia.

Objectives

To assess QT interval prolongation in 3 studies investigating once-daily pimavanserin as an adjunct to current antipsychotic treatment in patients with schizophrenia.

Methods

Electrocardiograms were unblinded from trials in which pimavanserin or placebo was added to main antipsychotics over 6 weeks (ENHANCE), 26 weeks (ADVANCE), and up to 78 weeks (ongoing 52-week, open-label extension study [study 035]) of treatment. Antipsychotic treatment was permitted throughout these studies. The 3 most frequently used antipsychotic treatments were examined—aripiprazole (including long-acting injectable), risperidone (including long-acting injectable), and olanzapine. QT intervals were corrected (QTc) using Fridericia's method, with elevated risk defined as either postbaseline value maximum of >500 ms or change from baseline to postbaseline maximum of >60 ms.

Results

Of patients treated with adjunctive pimavanserin in ENHANCE, there were no postbaseline QTc values >481 ms; one patient in each of the risperidone and aripiprazole groups had change from baseline to postbaseline maximum >60 ms. More patients had change from baseline to postbaseline maximum ranging from 31 to 60 ms in the risperidone plus adjunctive placebo group (n = 5; 6.6%) than those in the risperidone plus adjunctive pimavanserin group (n = 3, 4.1%). In the pimavanserin plus antipsychotic group of ADVANCE, one patient had postbaseline QTc value >481 ms, and one patient treated with aripiprazole had change from baseline to postbaseline maximum of >60 ms. In study 035, a change from double-blind baseline to overall postbaseline maximum >60 ms occurred in one patient treated with aripiprazole and pimavanserin and in one patient treated with risperidone and pimavanserin. Similar proportions of patients had changes from double-blind baseline to post double-blind baseline maximum between 31 and 60 ms across treatments. No adverse events associated with an increase in the QTc interval were reported.

Conclusions

Adjunctive pimavanserin with background antipsychotic treatment showed no evidence of QTc prolongation >500 ms postbaseline, consistent with previously reports on QT prolongation with pimavanserin.
Prevalence and associated factors of obesity and overweight in Chinese patients with bipolar disorder
Authors: Yi Wenying , Wu Haibo , Li Ruikeng , Li Haijing , Song Zhen , She Shenglin , Zheng Yingjun
Published: September 2022

Object

Despite abundant literature demonstrating a high prevalence of obesity and overweight in people with bipolar disorder (BD), little is known about this topic in China. Therefore, we assessed the prevalence and associated factors of obesity and overweight among inpatients with BD in our hospital, one of the largest public psychiatric hospitals in China.

Methods

In this retrospective, cross-sectional study, 1,169 inpatients ≥18 years with BD during 2019 were included. Obesity was defined as having a BMI ≥25 kg/m2, and overweight was defined as having a BMI from 23 kg/m2 to <25 kg/m2. Binary logistic regression analysis was performed to identify factors associated with obesity and overweight.

Results

The prevalence of obesity and overweight was 21.0% and 32.2% in patients with BD, respectively. Compared to patients with overweight and normal weight, patients with obesity were older, had a longer duration of BD and a longer length of hospital stay, had a higher prevalence of diabetes and hypertension, and had a higher level of all metabolic indices, except for HDL cholesterol. Binary logistic regression analysis showed that duration of BD, uric acid, alanine aminotransferase (ALT), triglyceride, and LDL cholesterol were significantly associated with obesity, and male sex and uric acid level were significantly associated with overweight (p < 0.05).

Conclusions

Obesity and overweight were fairly prevalent in Chinese BD patients, and several factors were related to obesity and overweight. The results of the present study call for the need to implement early screening, prevention and interventions for obesity and overweight in patients with BD in China.
A case report on mixed pulmonary infection of Nocardia nova, Mycobacterium tuberculosis, and Aspergillus fumigatus based on metagenomic next-generation sequencing
Authors: Yan Haiming , Li Zhandong , Xia Han , Li Qixin , Bai Honglian
Published: September 2022

Background

Pulmonary infection is one of the common complications of long-term use of glucocorticoids. Severe infections not only increase the length of hospital stay and treatment costs but also cause progression or recurrence of the primary disease.

Case description

Herein, we reported a case of mixed pulmonary infection secondary to glucocorticoid use. Rare pathogens such as Nocardia nova, Mycobacterium tuberculosis, Aspergillus fumigatus, and cytomegalovirus were detected by metagenomic next-generation sequencing (mNGS) of bronchoalveolar lavage fluid and lung puncture tissue. Combining the results of conventional pathogen detection and clinical symptoms, the patient was diagnosed with mixed pulmonary infection by multiple pathogens. After timely targeted medication, the patient was finally discharged with a good prognosis.

Conclusion

To our knowledge, this is the first case report on mixed pulmonary infection with pathogens including Nocardia nova, Mycobacterium tuberculosis, Aspergillus fumigatus, and human cytomegalovirus. As a new clinical diagnostic method, mNGS has great advantages in diagnosis of diseases such as mixed infections.
Access to care through telehealth among U.S. Medicare beneficiaries in the wake of the COVID-19 pandemic
Authors: Lu Min , Liao Xinyi
Published: September 2022

Background

The coronavirus disease 2019 (COVID-19) public health emergency has amplified the potential value of deploying telehealth solutions. Less is known about how trends in access to care through telehealth changed over time.

Objectives

To investigate trends in forgone care and telehealth coverage among Medicare beneficiaries during the COVID-19 pandemic.

Methods

A cross-sectional study design was used to analyze the outcomes of 31,907 Medicare beneficiaries using data from three waves of survey data from the Medicare Current Beneficiary Survey COVID-19 Supplement (Summer 2020, Fall 2020, and Winter 2021). We identified informative variables through a multivariate classification analysis utilizing Random Forest machine learning techniques.

Findings

The rate of reported forgone medical care because of COVID-19 decreased largely (22.89–3.31%) with a small increase in telehealth coverage (56.24–61.84%) from the week of June 7, 2020, to the week of April 4 to 25, 2021. Overall, there were 21.97% of respondents did not know whether their primary care providers offered telehealth services; the rates of forgone care and telehealth coverage were 11.68 and 59.52% (11.73 and 81.18% from yes and no responses). Our machine learning model predicted the outcomes accurately utilizing 43 variables. Informative factors included Medicare beneficiaries' age, Medicare-Medicaid dual eligibility, ability to access basic needs, certain mental and physical health conditions, and interview date.

Conclusions

This cross-sectional survey study found proliferation and utilization of telehealth services in certain subgroups during the COVID-19 pandemic, providing important access to care. There is a need to confront traditional barriers to the proliferation of telehealth. Policymakers must continue to identify effective means of maintaining continuity of care and growth of telehealth services.