bluuebunny/crossref_metadata_2025 huggingface dataset:
Keterikatan kepada Tuhan dan Pertumbuhan iman terhadap Komitmen Organisasi pada jemaat Gereja di Surabaya
Authors: Lontoh Frederich Oscar , Chia Philip , Widianto Kasiatin , Prasetyo Widi , Triono Triono
Published: November 2023

This research aims to determine whether there is an influence of attachement to to God and faith growth on Organizational Commitment among church congregations in Surabaya. The methodology used is quantitative with correlation analysis and multiple linear regression analysis. The population in this study consists of Christian church congregations in Surabaya, Indonesia, in accordance with predetermined respondent criteria. Respondents were selected through purposive random sampling. The instruments used in this research are the Closeness to God Scale, Faith Growth Scale, and Organizational Commitment Scale. Data analysis was conducted using partial correlation tests. The results of this study indicate a positive relationship between attachement  to God, faith growth, and Organizational Commitment among Christian church congregations in the city of Surabaya, Indonesia. Partially and collectively, Organizational Commitment can be influenced by closeness to God and faith growth, even though the values are relatively low. In other words, these two independent variables are not the primary factors in the lives of Christian church congregations that can enhance their commitment to the local church.

ABSTRAK BAHASA INDOENSIA

Penelitian ini bertujuan untuk mengetahui apakah ada pengaruh Keterikatan kepada Tuhan dan Pertumbuhan iman terhadap Komitmen Organisasi pada jemaat Gereja di Surabaya. Metodologi yang digunakan adalah kuantitaif dengan analsis korelasi dan analisis regresi liner berganda. Populasi dalam penelitian ini adalah jemaat gereja-gereja Kristen di Surabaya, Indonesia, sesuai dengan kristeria responden yang telah ditentukan. Responden dipilih secara purposive random sampling. Instrumen yang digunakan dalam penelitian ini adalah Skala Kedekatan pada Tuhan, Pertumbuhan iman dan Skala Komitmen Organisasi. Analisis data menggunakan uji korelasi parsial. Hasil penelitian ini menunjukkan ada hubungan yang poisitif antara  Keterikatan kepada Tuhan, Pertumbuhan iman dan Komitmen Organisasi pada jemaat gereja-gerja Kristen di kota Surabaya, Indonesia. Secara parsial dan secara bersama-sama Komitmen Organisasi dapat dipengaruhi oleh Kedekatan pada Tuhan dan Pertumbuhan iman sekalipun dengan nilai yang tergolong rendah.  Dengan kata lain bahwa kedua variable independent tersebut adalah bukan faktor utama dalam kehidupan jemaat-jemaat Gereja Kristen yang dapat meningkatkan Komitmen Organisasi mereka kepada gereja lokal.
Random walk and cell morphology dynamics in Naegleria gruberi
Authors: Uwamichi Masahito , Miura Yusuke , Kamiya Ayako , Imoto Daisuke , Sawai Satoshi
Published: November 2023

Amoeboid cell movement and migration are wide-spread across various cell types and species. Microscopy-based analysis of the model systems Dictyostelium and neutrophils over the years have uncovered generality in their overall cell movement pattern. Under no directional cues, the centroid movement can be quantitatively characterized by their persistence to move in a straight line and the frequency of re-orientation. Mathematically, the cells essentially behave as a persistent random walker with memory of two characteristic time-scale. Such quantitative characterization is important from a cellular-level ethology point of view as it has direct connotation to their exploratory and foraging strategies. Interestingly, outside the amoebozoa and metazoa, there are largely uncharacterized species in the excavate taxon Heterolobosea including amoeboflagellate Naegleria. While classical works have shown that these cells indeed show typical amoeboid locomotion on an attached surface, their quantitative features are so far unexplored. Here, we analyzed the cell movement of Naegleria gruberi by employing long-time phase contrast imaging that automatically tracks individual cells. We show that the cells move as a persistent random walker with two time-scales that are close to those known in Dictyostelium and neutrophils. Similarities were also found in the shape dynamics which are characterized by the appearance, splitting and annihilation of the curvature waves along the cell edge. Our analysis based on the Fourier descriptor and a neural network classifier point to importance of morphology features unique to Naegleria including complex protrusions and the transient bipolar dumbbell morphologies.
Causal effects of nonalcoholic fatty liver disease on cerebral cortical structure: a Mendelian randomization analysis
Authors: Mai Zhiliang , Mao Hua
Published: November 2023

Background

Previous studies have highlighted changes in the cerebral cortical structure and cognitive function among nonalcoholic fatty liver disease (NAFLD) patients. However, the impact of NAFLD on cerebral cortical structure and specific affected brain regions remains unclear. Therefore, we aimed to explore the potential causal relationship between NAFLD and cerebral cortical structure.

Methods

We conducted a Mendelian randomization (MR) study using genetic predictors of alanine aminotransferase (ALT), NAFLD, and percent liver fat (PLF) and combined them with genome-wide association study (GWAS) summary statistics from the ENIGMA Consortium. Several methods were used to assess the effect of NAFLD on full cortex and specific brain regions, along with sensitivity analyses.

Results

At the global level, PLF nominally decreased SA of full cortex; at the functional level, ALT presented a nominal association with reduced SA of parahippocampal gyrus, TH of pars opercularis, TH of pars orbitalis, and TH of pericalcarine cortex. Besides, NAFLD presented a nominal association with reduced SA of parahippocampal gyrus, TH of pars opercularis, TH of pars triangularis and TH of pericalcarine cortex, but increased TH of entorhinal cortex, lateral orbitofrontal cortex and temporal pole. Furthermore, PLF presented a nominal association with reduced SA of parahippocampal gyrus, TH of pars opercularis, TH of cuneus and lingual gyrus, but increased TH of entorhinal cortex.

Conclusion

NAFLD is suggestively associated with atrophy in specific functional regions of the human brain.
Construction and optimization of ecological security pattern based on landscape ecological risk assessment in the affected area of the Lower Yellow River
Authors: Huang Yicheng , Zhang Jinbing , Zhang Pengyan , Chen Zhuo , Zhang Xinyue , Lu Rong , Li Mengfan , Xing Guangrui , Song Yongpeng
Published: November 2023

In the context of urban expansion and climate change, the world is under pressure from multiple ecological risks. Key ecological protection areas play a pivotal role in preserving ecological stability and promoting development. Due to its unique geographical conditions, the Yellow River basin has been facing huge ecological risk pressure. In the affected area of the Lower Yellow River (AALYR) as an agricultural hub, ecological protection has gradually become a key factor restricting the development of cities and agriculture. Taking AALYR as an example, the landscape ecological risk assessment (LERA) system is established based on three aspects “natural environment—human society—landscape pattern”. We construct a comprehensive cumulative resistance surface based on the risk assessment results as the basis for the future study. Ecological corridors are identified by minimum cumulative resistance (MCR) models to establish and optimize Ecological security pattern (ESP) in the AALYR. We found that the landscape ecological risks (LER) in the study area show a uniform spatial distribution, with a slightly higher distribution in the northeast than the southwest. The ecological risk levels are generally high in AALYR, indicating a more severe risk problem in this area. A total of 56 ecological sources were identified, with a total area of 21176 km2. The ecological sensitivity of AALYR was high, and 99 ecological corridors and 59 ecological nodes were extracted. Ecological corridors and nodes were consistently and densely distributed throughout the study area. The network analysis method improves the stability of the network structure after optimization. Based on the key components of the ESP, with the combination of geographical characteristics and local policy planning guidance, we constructed the “One Belt and One Axis, Two Cores and Two Corridors, Four zones” ESP. The study results may offer guidance and suggestions for the construction of ESP and ecological environment protection system in the world’s major river basins, and may also provide information for ecological planning of other similar river basins in the world.
Chromosome-level genome assembly of the largefin longbarbel catfish (Hemibagrus macropterus)
Authors: Ye Huan , Fan Jiahui , Hou Yanling , Yue Huamei , Ruan Rui , Li Shuang , Hu Chongjiang , Xie Yong , Li Chuangju
Published: November 2023

The largefin longbarbel catfish, Hemibagrus macropterus, is an economically important fish species in southwestern China, with males growing faster than females. This study presents a high-quality chromosome-level genome assembly of the largefin longbarbel catfish, generated by integrating Illumina short reads, PacBio HiFi long reads, and Hi-C data. The assembled genome size was 858.5 Mb, with a contig and scaffold N50 of 5.8 Mb and 28.4 Mb, respectively. A total of 656 contigs were successfully anchored to 30 pseudochromosomes with a BUSCO score of 97.7%, consistent with the number of chromosomes analyzed by karyotype. The genome contained 29.5% repeat sequences, and a predicted total of 26,613 protein-coding genes, of which 25,769 (96.8%) were functionally annotated in different databases. Evolutionary analysis showed that H. macropterus was most closely related to H. wyckioides, with a divergence time of approximately 16.3 million years. Chromosomal syntenic relationships among H. macropterus, H. wyckioides, and Pelteobagrus fulvidraco revealed a one-to-one relationship for most chromosomes, except for break, fission, and inversion of some chromosomes. The first high-quality reference genome will not only provide a valuable genetic resource for the study of sex determination mechanisms and genetic breeding of largefin longbarbel catfish, but also contribute to comparative analyses of genome and chromosome evolution within Siluriformes.
α-Glucosidase inhibitors boost gut immunity by inducing IgA responses in Peyer’s patches
Authors: Hattori-Muroi Kisara , Naganawa-Asaoka Hanako , Kabumoto Yuma , Tsukamoto Kei , Fujisaki Yosuke , Fujimura Yumiko , Komiyama Seiga , Kinashi Yusuke , Kato Miki , Sato Shintaro , Takahashi Daisuke , Hase Koji
Published: November 2023

Peyer’s patches (PPs) are specialized gut-associated lymphoid tissues that initiate follicular helper T (Tfh)-mediated immunoglobulin A (IgA) response to luminal antigens derived from commensal symbionts, pathobionts, and dietary sources. IgA-producing B cells migrate from PPs to the small intestinal lamina propria and secrete IgA across the epithelium, modulating the ecological balance of the commensal microbiota and neutralizing pathogenic microorganisms. α-glucosidase inhibitors (α-GIs) are antidiabetic drugs that inhibit carbohydrate digestion in the small intestinal epithelium, leading to alterations in the commensal microbiota composition and metabolic activity. The commensal microbiota and IgA responses exhibit bidirectional interactions that modulate intestinal homeostasis and immunity. However, the effect of α-GIs on the intestinal IgA response remains unclear. We investigated whether α-GIs affect IgA responses by administering voglibose and acarbose to mice via drinking water. We analyzed Tfh cells, germinal center (GC) B cells, and IgA-producing B cells in PPs by flow cytometry. We also assessed pathogen-specific IgA responses. We discovered that voglibose and acarbose induced Tfh cells, GCB cells, and IgA-producing B cells in the PPs of the proximal small intestine in mice. This effect was attributed to the modification of the microbiota rather than a shortage of monosaccharides. Furthermore, voglibose enhanced secretory IgA (S-IgA) production against attenuated Salmonella Typhimurium. Our findings reveal a novel mechanism by which α-GIs augment antigen-specific IgA responses by stimulating Tfh-GCB responses in PPs, and suggest a potential therapeutic application as an adjuvant for augmenting mucosal vaccines.
The effect of anaerobic remineralization of the seagrass Halophila stipulacea on porewater biogeochemistry in the Gulf of Aqaba
Authors: Soto Neta , Winters Gidon , Antler Gilad
Published: November 2023

Introduction

Seagrasses form oxidizing microenvironments around their roots, creating complex and strong redox gradients, thus affecting the rates of microbial carbon mineralization in their surrounding sediments. Since seagrasses are continuously being lost worldwide, a deeper understanding of the changes that occur within different seagrass sediments following the disappearance of the plants is of ecological and global importance.

Methods

We conducted a slurry experiment with sediments that have different characteristics from the northern tip of Gulf of Aqaba; the different sediments included different compartments of the tropical seagrass Halophila stipulacea (old and young leaves, rhizomes, or roots). We measured the changes over time in dissolved inorganic carbon (DIC), alkalinity, ferrous iron (Fe2+), hydrogen sulfide (H2S), sulfate (SO42-), and sulphur isotope ratios in sulfate within water. These measurements were used to calculate the rate of remineralization of each seagrass compartment, allowing us to predict the potential effects of the disappearance of different H. stipulacea compartments on key microbial processes in the surrounding environment.

Results

We show that H. stipulacea’s rhizomes had the fastest decomposition rates, followed by the young leaves, roots, and old leaves (which also indicates the preservation potential of old leaves).

Discussion

High concentrations of hydrogen sulfide were detected only in the slurries containing rhizomes and young leaves. High sulfide concentrations can lead to seagrass mortality and cause a positive feedback loop where the loss of seagrass due to sulfide generates further sulfide accumulation. This positive feedback loop can also be further reinforced by the loss of burrowing fauna in the sediment. This emphasizes the importance of understanding the extent of different pathways of seagrass disappearance on the surrounding environment and other geochemical feedbacks.
Life cycle exposure to 2-phenylbenzimidazole-5-sulfonic acid disrupts reproductive endocrine system and induces transgenerational adverse effects in zebrafish
Authors: Tao Junyan , Yang Qinyuan , Sun Xiaowei , Tian Linxuan , Deng Yuanzhi , Wang Yumei , Wang Weiwei , Fan Xiaoming
Published: November 2023

Global attention has been focused on organic UV filters due to their ubiquity and potential damage to aquatic environment, yet the effects of their life cycle exposure on fish reproduction remain unknown. In the present study, the influence of 2-phenylbenzimidazole-5-sulfonic acid (PBSA) exposure on the reproductive endocrine system of zebrafish was examined, from 6 hours post fertilization (hpf) until 150 days, at levels that near-environmentally relevant (0 to 20 μg/L). Our results showed that exposure to PBSA at 20 μg/L caused a slight decrease in the Gonadosomatic Index (GSI) of female zebrafish in the F0 generation. Furthermore, this exposure had a negatively effect on reproduction, accompanied by delayed oocyte maturation, reduced cumulative egg production and decreased fertilization rate. Additionally, offspring embryos displayed reduced egg diameter at 0.75 hpf, delayed cumulative hatching rate at 60 hpf, and increased deformities rate at 72 hpf, indicating an adverse transgenerational effect. Moreover, PBSA exposure was associated with decreased plasma levels of sex hormones of 17β-estradiol (E2) and 11-ketotestosterone (11-KT), as well as altered the transcriptional profiles of certain genes in the HPG (hypothalamic–pituitary–gonadal) and liver axis. Molecular docking (MD) simulations revealed that specific amino acid residues of PBSA interact with zebrafish estrogen receptors, confirming its xenoestrogenic properties. Therefore, exposure to PBSA during its life cycle can disturb fish reproduction through endocrine disruption, thus necessitating strict environmental regulations for the disposal of UV filters to protect ecological and public health.
Dynamic geospatial modeling of mycotoxin contamination of corn in Illinois: unveiling critical factors and predictive insights with machine learning
Authors: Castano-Duque Lina , Winzeler Edwin , Blackstock Joshua M. , Liu Cheng , Vergopolan Noemi , Focker Marlous , Barnett Kristin , Owens Phillip Ray , van der Fels-Klerx H. J. , Vaughan Martha M. , Rajasekaran Kanniah
Published: November 2023

Mycotoxin contamination of corn is a pervasive problem that negatively impacts human and animal health and causes economic losses to the agricultural industry worldwide. Historical aflatoxin (AFL) and fumonisin (FUM) mycotoxin contamination data of corn, daily weather data, satellite data, dynamic geospatial soil properties, and land usage parameters were modeled to identify factors significantly contributing to the outbreaks of mycotoxin contamination of corn grown in Illinois (IL), AFL >20 ppb, and FUM >5 ppm. Two methods were used: a gradient boosting machine (GBM) and a neural network (NN). Both the GBM and NN models were dynamic at a state-county geospatial level because they used GPS coordinates of the counties linked to soil properties. GBM identified temperature and precipitation prior to sowing as significant influential factors contributing to high AFL and FUM contamination. AFL-GBM showed that a higher aflatoxin risk index (ARI) in January, March, July, and November led to higher AFL contamination in the southern regions of IL. Higher values of corn-specific normalized difference vegetation index (NDVI) in July led to lower AFL contamination in Central and Southern IL, while higher wheat-specific NDVI values in February led to higher AFL. FUM-GBM showed that temperature in July and October, precipitation in February, and NDVI values in March are positively correlated with high contamination throughout IL. Furthermore, the dynamic geospatial models showed that soil characteristics were correlated with AFL and FUM contamination. Greater calcium carbonate content in soil was negatively correlated with AFL contamination, which was noticeable in Southern IL. Greater soil moisture and available water-holding capacity throughout Southern IL were positively correlated with high FUM contamination. The higher clay percentage in the northeastern areas of IL negatively correlated with FUM contamination. NN models showed high class-specific performance for 1-year predictive validation for AFL (73%) and FUM (85%), highlighting their accuracy for annual mycotoxin prediction. Our models revealed that soil, NDVI, year-specific weekly average precipitation, and temperature were the most important factors that correlated with mycotoxin contamination. These findings serve as reliable guidelines for future modeling efforts to identify novel data inputs for the prediction of AFL and FUM outbreaks and potential farm-level management practices.
Neurexin and neuroligins jointly regulate synaptic degeneration at the Drosophila neuromuscular junction based on TEM studies
Authors: Guangming Gan , Mei Chen , Qinfeng Yu , Xiang Gao , Chenchen Zhang , Qingyuan Sheng , Wei Xie , Junhua Geng
Published: November 2023

The Drosophila larval neuromuscular junction (NMJ) is a well-known model system and is often used to study synapse development. Here, we show synaptic degeneration at NMJ boutons, primarily based on transmission electron microscopy (TEM) studies. When degeneration starts, the subsynaptic reticulum (SSR) swells, retracts and folds inward, and the residual SSR then degenerates into a disordered, thin or linear membrane. The axon terminal begins to degenerate from the central region, and the T-bar detaches from the presynaptic membrane with clustered synaptic vesicles to accelerate large-scale degeneration. There are two degeneration modes for clear synaptic vesicles. In the first mode, synaptic vesicles without actin filaments degenerate on the membrane with ultrafine spots and collapse and disperse to form an irregular profile with dark ultrafine particles. In the second mode, clear synaptic vesicles with actin filaments degenerate into dense synaptic vesicles, form irregular dark clumps without a membrane, and collapse and disperse to form an irregular profile with dark ultrafine particles. Last, all residual membranes in NMJ boutons degenerate into a linear shape, and all the residual elements in axon terminals degenerate and eventually form a cluster of dark ultrafine particles. Swelling and retraction of the SSR occurs prior to degradation of the axon terminal, which degenerates faster and with more intensity than the SSR. NMJ bouton degeneration occurs under normal physiological conditions but is accelerated in Drosophila neurexin (dnrx) dnrx273, Drosophila neuroligin (dnlg) dnlg1 and dnlg4 mutants and dnrx83;dnlg3 and dnlg2;dnlg3 double mutants, which suggests that both neurexin and neuroligins play a vital role in preventing synaptic degeneration.
Low geriatric nutritional risk index as a poor prognostic biomarker for immune checkpoint inhibitor treatment in solid cancer
Authors: Zhang Lilong , Wang Kunpeng , Kuang Tianrui , Deng Wenhong , Hu Peng , Wang Weixing
Published: November 2023

Objective

In this investigation, we focused on the geriatric nutritional risk index (GNRI), a comprehensive metric that takes into account the patient’s ideal weight, actual weight, and serum albumin levels to measure malnutrition. Our primary objective was to examine the predictive value of GNRI-defined malnutrition in determining the response to immunotherapy among cancer patients.

Methods

Relevant articles for this study were systematically searched in PubMed, the Cochrane Library, EMBASE, and Google Scholar up to July 2023. Our analysis evaluated overall survival (OS), progression-free survival (PFS), objective response rate (ORR), and disease control rate (DCR) as clinical outcomes.

Results

This analysis comprised a total of eleven articles encompassing 1,417 patients. The pooled results revealed that cancer patients with low GNRI levels exhibited shorter OS (HR: 2.64, 95% CI: 2.08–3.36, p < 0.001) and PFS (HR: 1.87, 95% CI: 1.46–2.41, p < 0.001), and lower ORR (OR: 0.46, 95% CI: 0.33–0.65, p < 0.001) and DCR (OR: 0.42, 95% CI: 0.29–0.61, p < 0.001). Sensitivity analyses confirmed that the above results were stable. Egger’s and Begg’s tests revealed that there was no publication bias in the above results.

Conclusion

Our results imply that the GNRI is a useful predictor of immunotherapy response in cancer patients.
Two case reports: EML4-ALK rearrangement large cell neuroendocrine carcinoma and literature review
Authors: Chen Qin , Zhang Jingjing , Wang Xuan , Zong Wenkang , Sun Leina , Qin Jianwen , Yin Yan
Published: November 2023

Anaplastic lymphoma kinase gene (ALK) rearrangement is present in only approximately 5% of non-small cell lung cancers (NSCLCs) and is scarce in LCNEC patients. The conventional first-line treatment options are chemotherapy combined with immunotherapy or chemotherapy followed by palliative radiotherapy. In this report, we present two cases of metastatic LCNEC with EML4-ALK fusion that were treated with ALK-TKI inhibitors and demonstrated a rapid therapeutic response. Both patients were nonsmoking women who declined cytotoxic chemotherapy, underwent Next-Generation Sequencing (NGS), and confirmed EML4-ALK fusion. They were treated with alectinib as first-line therapy, and the tumors showed significant shrinkage after two months, achieving a PR (defined as a more than 30% decrease in the sum of maximal dimensions). The PFS was 22 months and 32 months, respectively, until the last follow-up. A systematic review of all previously reported cases of LCNEC with ALK mutations identified only 21 cases. These cases were characterized by being female (71.4%), nonsmoking (85.7%), diagnosed at a relatively young age (median age 51.1), and stage IV (89.5%), with an overall response rate (ORR) of 90.5%. PFS and OS were significantly longer than those treated with conventional chemotherapy/immunotherapy. Based on the clinical characteristics and the effective therapeutic outcomes with ALK inhibitors in LCNEC patients with ALK fusion, we recommend routine ALK IHC (economical, affordable, and convenient, but with higher false positives) as a screening method in advanced LCNEC patients, particularly nonsmoking females or those who are not candidates for or unwilling to undergo cytotoxic chemotherapy. Further molecular profiling is necessary to confirm these potential beneficiaries. We suggest TKI inhibitors as the first-line treatment for metastatic LCNEC with ALK fusion. Additional studies on larger cohorts are required to assess the prevalence of ALK gene fusions and their sensitivity to various ALK inhibitors.
Case Report: A novel FGFR1 fusion in acute B-lymphoblastic leukemia identified by RNA sequencing
Authors: Zhang Zhibo , Zhu Yiyan , Wang Zheng , Zeng Zhao , Wen Lijun , Zhang Ling , Chen Suning
Published: November 2023

8p11 myeloproliferative syndrome is a rare hematological malignancy with aggressive course caused by the various translocation of FGFR1. In this study, a novel FGFR1 fusion was identified by RNA sequencing in a 28-year-old male patient with acute B-lymphoblastic leukemia. The patient harbors an in-frame fusion between KIF5B exon 15 and FGFR1 exon 10. The FGFR1 fusion and its protein expression was validated by Sanger sequencing and Western blot. Meanwhile, cytogenetic analysis reported a normal karyotype and targeted DNA sequencing identified no driver mutations, respectively. Despite he achieved complete remission after induction regimen, a relapse occurred and he became refractory to chemotherapy, and salvage haploidentical hematopoietic stem cell transplantation failed to control the progressive disease. In conclusion, we present the first case of KIF5B-FGFR1 fusion in hematological malignancy. These findings extend the spectrum of translocation in 8p11 myeloproliferative syndrome, and demonstrate the great prospect of RNA sequencing in clinical practice again.
Overnight staffing in Canadian neonatal and pediatric intensive care units
Authors: Maratta Christina , Hutchison Kristen , Nicoll Jessica , Bagshaw Sean M. , Granton John , Kirpalani Haresh , Stelfox Henry Thomas , Ferguson Niall , Cook Deborah , Parshuram Christopher S. , Moore Gregory P.
Published: November 2023

Aim

Infants and children who require specialized medical attention are admitted to neonatal and pediatric intensive care units (ICUs) for continuous and closely supervised care. Overnight in-house physician coverage is frequently considered the ideal staffing model. It remains unclear how often this is achieved in both pediatric and neonatal ICUs in Canada. The aim of this study is to describe overnight in-house physician staffing in Canadian pediatric and level-3 neonatal ICUs (NICUs) in the pre-COVID-19 era.

Methods

A national cross-sectional survey was conducted in 34 NICUs and 19 pediatric ICUs (PICUs). ICU directors or their delegates completed a 29-question survey describing overnight staffing by resident physicians, fellow physicians, nurse practitioners, and attending physicians. A comparative analysis was conducted between ICUs with and without in-house physicians.

Results

We obtained responses from all 34 NICUs and 19 PICUs included in this study. A total of 44 ICUs (83%) with in-house overnight physician coverage provided advanced technologies, such as extracorporeal life support, and included all ICUs that catered to patients with cardiac, transplant, or trauma conditions. Residents provided the majority of overnight coverage, followed by the Critical Care Medicine fellows. An attending physician was in-house overnight in eight (15%) out of the 53 ICUs, seven of which were NICUs. Residents participating in rotations in the ICU would often have rotation durations of less than 6 weeks and were often responsible for providing care during shifts lasting 20–24 h.

Conclusion

Most PICUs and level-3 NICUs in Canada have a dedicated in-house physician overnight. These physicians are mainly residents or fellows, but a notable variation exists in this arrangement. The potential effects on patient outcomes, resident learning, and physician satisfaction remain unclear and warrant further investigation.
Safety, tolerability, and pharmacokinetics of TG-1000, a new molecular entity against influenza virus: first-in-human study
Authors: Xu Su-Mei , Chang Li-Wen , Tsai Cheng-Yuan , Liu Wan-Li , Li Dai , Li Shan-Shan , Li Xiao-Min , Xu Ping-Sheng
Published: November 2023

Background: The cap-snatching mechanism of influenza virus mRNA transcription is strongly suppressed by TG-1000, a prodrug rapidly metabolized into TG-0527, is a potent cap-dependent nucleic acid endonuclease inhibitor. Herein, we aimed to assess the safety, tolerability, and pharmacokinetics of TG-1000 in healthy participants and the effect of food on the pharmacokinetics and safety of TG-1000.

Method: The study was divided into 2 parts: Part A [Single Ascending-Dose (SAD) study, 10–160 mg] and Part B [Food-Effect (FE) study, 40 mg] were launched sequentially. The study included 66 participants for both investigations. We administered different TG-1000 capsules or placebo doses per the study protocol and collected blood samples for pharmacokinetic assessments at specific times. In plasma, TG-1000 and its active metabolite TG-0527 were assayed, and PK parameters were determined.

Results: In SAD, the increase in AUC was less than the proportional increase in dose over the 20–160 mg dose range, while the increase in Cmax was proportional to the increase in dose. In the 10–160 mg dose range, T1/2, λz and Tmax of TG-0527 were dose-independent; and T1/2 and Tmax were within 33.8–39.4 h and 3.02–6 h, respectively. In FE, the AUC0-inf, AUC0-last, and Cmax of TG-0527 decreased by approximately 17.52%, 18.76%, and 41.35%, respectively, and the Tmax delay was around 1.50 h. No serious adverse events occurred during the studies.

Conclusion: Overall, TG-1000 was well tolerated and exhibited an acceptable safety and PK profile, supporting further clinical investigation of TG-1000 for the treatment of influenza.
The Me and the Us of Emotions: a cluster-randomized controlled trial of the feasibility and efficacy of a compassion-based social–emotional learning program for children
Authors: Xavier Ana , Vagos Paula , Palmeira Lara , Menezes Paulo , Patrão Bruno , Mendes Sofia Abreu , Tavares Marta
Published: November 2023

There are well-established benefits of social and emotional learning (SEL) programs for children within educational contexts. Combining social–emotional skills and compassion abilities has been seldomly done, and it may be valuable at individual and societal levels, for resilient, empathetic, and inclusive societies. This study explored the feasibility and efficacy of a program designed to promote socioemotional and compassion skills in children attending the 3rd and 4th grades, by using in-class dynamics complemented with serious games. This program, named “The Me and the Us of Emotions,” is part of the Gulbenkian Knowledge Academies 2020 and consists of 10 group sessions embedded in the school curriculum. Using a cluster-randomized controlled trial design, school classes were allocated to intervention (classes, n = 8; children, n = 163) and control groups (classes, n = 6; children, n = 132). During the program, facilitators assessed adherence to the sessions’ plan, attendance, dosage (i.e., how many sessions were delivered), and participant responsiveness. Children completed self-report measures of social–emotional skills and emotional climate at pre-, post-intervention, 3-month, and 6-month follow-ups. Results indicate that the program is feasible, with high adherence, high attendance rate, and participant responsiveness. Results also indicate empathy, soothing, and drive feelings to change from pre-intervention to all other assessment moments, for the intervention group only. Moreover, cooperation and threat changed over time for participants in both the control and the intervention groups. The current study offers empirical support for the feasibility and utility of a compassion-based social–emotional learning program on promoting children’s empathy, and emotions of soothing and vitality in the school context. Thus, these findings contribute to recent research on the potential added value of compassion practices within an SEL program.
Attending to what’s important: what heat maps may reveal about attention, inhibitory control, and fraction arithmetic performance
Authors: Godwin Karrie E. , Thompson Clarissa A. , Kaur Freya , Iwai Yuika , Fitzsimmons Charles J. , Taber Jennifer M.
Published: November 2023

Math proficiency is an important predictor of educational attainment and life success. However, developing mathematical competency is challenging, and some content (e.g., fractions) can be enigmatic. Numerous factors are suspected to influence math performance, including strategy knowledge, attention, and executive functions. In two online studies, we investigated the relationship between adults’ fraction arithmetic performance, confidence judgments, inhibitory control (a component of executive functions), and attention to strategy-relevant fraction components. We explored the utility of heat maps (based on mouse clicks) to measure adults’ attention to strategy-relevant fraction arithmetic components (operationalized according to each mathematical operation). In Study 1, attending to strategy-relevant fraction components was correlated with inhibitory control, but this finding did not replicate in Study 2. Across both studies, inhibitory control and attention to strategy-relevant fraction components were correlated with arithmetic accuracy. Intraindividual variability in participants’ attention to strategy-relevant fraction components was also found. Our findings suggest that heat map questions may be a viable alternative to assess participants’ attention during fraction tasks and that attention to specific fraction-arithmetic problem features is related to problem-solving accuracy.
Adolescents and young adults with oncohematological disease: use of social networks, impact of SARS-COV-2, and psychosocial well-being
Authors: Tremolada Marta , Taverna Livia , Vietina Francesco , Incardona Roberta Maria , Pierobon Marta , Bonichini Sabrina , Biffi Alessandra , Bisogno Gianni
Published: November 2023

Objective

Adolescents and young adults (AYA) with oncohematological diseases could have important psychosocial difficulties that could be worsened by the effects of the COVID19 pandemic. At this developmental stage, it is also important to assess the use of social networks (SNs). This study aims to investigate the type of social network use and the consequences of the COVID19 period. These patients are compared with matched healthy peers.

Methods

After the informant consent signature, the adolescents completed a series of self-report questionnaires on the use of SNs, on communication preferences, on social anxiety and on Covid19 impact through the online platform of LimeSurvey. Most of the adolescents belonged to the 18–20 age group (42.5%), were female (62%) and mainly off therapy (72%).

Results

Adolescents spent more than 2 h/day on Instagram and 1 h and half on Whatsapp, while Tik Tok use was on average 1 h/day, especially used by younger patients (r = −0.33, p = 0.023). Males used Twitch (t45 = −2.06, p = 0.05) and Youtube (t45 = −2.18, p = 0.03) for longer than females. AYA in therapy used more Tik Tok (U = 137.50; p = 0.03), Ask/Tellonym (U = 172.50; p = 0.05) and Twitch (U = 144; p = 0.017) than those off therapy. Healthy AYA showed lower levels of exposure (Z = −4.17; p = 0.00001) and impact (Z = −5.12; p = 0.00001) to Covid19, while the level of social anxiety is comparable and is in the normal range in both groups.

Discussion

Some clinical considerations and suggestions could be given based on these empirical results to health professionals in the care of AYA cancer patients.
Routine immunization experience and practices during the COVID-19 pandemic of caregivers attending a tertiary hospital in Cape Town
Authors: Manan A. , Wessels T. , Finlayson H.
Published: November 2023

Introduction

Immunizations are successful, cost-effective interventions for the control of infectious diseases and preventing mortality. Lockdown restrictions during the COVID-19 pandemic had adverse effects on child-health including access to immunizations. Our study aimed to document immunization status, describe caregiver experiences around accessing immunizations during the COVID-19 pandemic and identify any significant factors associated with immunization status.

Methods

Caregivers, with children between the ages of 10 to 33 months, attending Tygerberg Hospital Paediatric Department were invited to complete an anonymous survey from 15th September–15th December 2022. Data was captured using a REDCap questionnaire and analysed using Stata Version 17.

Results

171 caregivers completed the survey. Immunizations were up to date in 81%. Most (155, 88%) agreed it was important to immunize their child. A third of caregivers (55) felt it was unsafe to attend the clinic and 37% (62) agreed it was difficult to attend. Caregivers receiving a social grant (p = 0.023) or who felt safe attending clinic (p = 0.053) were more likely to be up to date with immunizations. Three-quarters (128, 78%) were aware of recommendations to continue immunization. These caregivers were more likely to think it was important to immunize on time (p = 0.003) and to receive family encouragement (p = 0.001). Caregivers were more likely to attend clinic if they felt it was important to vaccinate on time (p < 0.001) or felt safe attending clinic (p = 0.036).

Conclusion

Immunization rates were higher than expected but below global targets. Although caregivers feel immunizations are important, unknowns still instilled fear of attending clinics. Social factors such as family support and social grants improve vaccine seeking behaviour.
Eco-innovative technology for wastewater treatment and reuse in MENA region: case of Lebanon
Authors: El Moll Ahmad
Published: November 2023

Water scarcity is a significant problem worldwide especially in Middle East and North Africa, to which the reuse of wastewater after appropriate treatment could offer a sustainable solution. With recent advances in wastewater treatment technologies and reuse, it is possible to obtain wastewater effluent of consistently high quality, which can then be reused in the agricultural sector. Thus, facing of water shortage, deficiency of sanitation and pollution by wastewater in the Mediterranean region, the decentralization using eco-innovative technology, focuses mainly on the wastewater treatment on-site and on the local recycling and reuse of resources contained in domestic wastewater, represents a real and well solution. Therefore, sustainable solutions for wastewater management will be discussed, with a focus on the role of innovative technology with detailed description of the wastewater treatment process. Additionally, the implications of wastewater reuse in agriculture sector in the context of the circular economy as potential solutions will also be presented in this paper.
A Review of Phytochemicals and Bioactive Properties in the Proteaceae Family: A Promising Source of Functional Food
Authors: Zhang Jiale , Netzel Michael E. , Pengelly Andrew , Sivakumar Dharini , Sultanbawa Yasmina
Published: November 2023

In recent decades, natural plant-based foods have been increasingly used to improve human health due to unhealthy modern dietary patterns, such as the consumption of foods high in sugar and fat. Many indigenous species have been used by Aboriginal peoples for their food and therapeutic properties. Thus, it is important to understand the health-enhancing bioactive profile of Australian indigenous species. The Proteaceae family, such as the genera of Protea, Macadamia, and Grevillea, have been commercially used in the horticulture and food industries. Researchers have reported some findings about Persoonia species, one of the genera in the Proteaceae family. The aim of this review was to provide an overview of the family Proteaceae and the genus Persoonia, including distribution, traditional and commercial uses, phytochemicals, bioactive properties, potential opportunities, and challenges. In this review, bioactive compounds and their properties related to the health benefits of the Proteaceae family, particularly the Persoonia genus, were reviewed for potential applications in the food industry.
Sorbets as Functional Food Products, Unexplored Food Matrices, Their Challenges, and Advancements
Authors: Williams Jackson , McKune Andrew J. , Naumovski Nenad
Published: November 2023

Functional foods and beverages are becoming one of the leading food products on the global market today. This is predominately due to the consumer, industry and research-related interests in the use of food-derived products for the management of several chronic conditions. The diversity of currently available functional food products also provides an opportunity for the use of fruit-based sorbets as a carrier of functional ingredients. Therefore, the aim of this literature review is to explore the use of sorbets as a functional food product, which is one commercial method that can be utilized to provide health benefits, extend the shelf life of foods, and preserve nutrients and improve taste. Firstly, we provide an overview of sorbets as a functional food matrix, their development and implications for the absorption of functional ingredients in humans. We discuss the developmental considerations of functional foods, such as the technical conditions and physicochemical and organoleptic properties through which functional foods can provide beneficial health effects. These include product stability, metabolism of the functional food ingredient, its interactions with the food matrix and limitations related to its production. There is a paucity of clinical data that investigate the long-term health effects of products claiming additional functional benefits. Given the extensive potential benefits of functional bioactive food compounds and their heavy prevalence in the market, extensive research and further regulation is needed to ensure health recommendations for large populations in longitudinal clinical studies warranting any functional claim.
Multi-Agent Collaborative Target Search Based on the Multi-Agent Deep Deterministic Policy Gradient with Emotional Intrinsic Motivation
Authors: Zhang Xiaoping , Zheng Yuanpeng , Wang Li , Abdulali Arsen , Iida Fumiya
Published: November 2023

Multi-agent collaborative target search is one of the main challenges in the multi-agent field, and deep reinforcement learning (DRL) is a good way to learn such a task. However, DRL always faces the problem of sparse reward, which to some extent reduces its efficiency in task learning. Introducing intrinsic motivation has proved to be a useful way to make the sparse reward in DRL. So, based on the multi-agent deep deterministic policy gradient (MADDPG) structure, a new MADDPG algorithm with the emotional intrinsic motivation name MADDPG-E is proposed in this paper for the multi-agent collaborative target search. In MADDPG-E, a new emotional intrinsic motivation module with three emotions, joy, sadness, and fear, is designed. The three emotions are defined by corresponding psychological knowledge to the multi-agent embodied situations in an environment. An emotional steady-state variable function H is then designed to help judge the goodness of the emotions. Based on H, an emotion-based intrinsic reward function is finally proposed. With the designed emotional intrinsic motivation module, the multi-agent system always tries to make itself joy, which means it always learns to search the target. To show the effectiveness of the proposed MADDPG-E algorithm, two kinds of simulation experiments with a determined initial position and random initial position, respectively, are carried out, and comparisons are performed with MADDPG as well as MADDPG-ICM (MADDPG with an intrinsic curiosity module). The results show that with the designed emotional intrinsic motivation module, MADDPG-E has a higher learning speed and better learning stability, and the advantage is more obvious when facing complex situations.
Episodic Vertigo: A Narrative Review Based on a Single-Center Clinical Experience
Authors: Casani Augusto Pietro , Gufoni Mauro , Ducci Nicola
Published: November 2023

(1) Background: Usually, the majority of patients suffering from vertigo and dizziness can be identified in four major categories: acute spontaneous vertigo, episodic (recurrent) vertigo, recurrent positional vertigo, and chronic imbalance. Our purpose is to retrospectively evaluate the main causes of episodic vertigo and to find indications for a reliable clinical suspicion useful for a definitive diagnosis, comparing patients affected by different presenting symptomatology (acute vertigo, recurrent episodic vertigo, and imbalance). (2) Methods: we retrospectively evaluated the clinical records in a population of 249 consecutive patients observed for vertigo in our tertiary referral center in the period 1 January 2019–31 January 2020. On the basis of the reported clinical history, patients were divided into three groups: patients with their first ever attack of vertigo, patients with recurrent vertigo and dizziness, and patients with chronic imbalance. (3) Results: On the basis of the results of the instrumental examination, we arbitrarily divided (for each type of symptoms) the patients in a group with a normal vestibular instrumental examination and a group of patients in which the clinical–instrumental evaluation showed some pathological results; a highly significant difference (p: 0.157) was found between recurrent and acute vertigo and between recurrent vertigo and imbalance. (4) Conclusions: Patients with recurrent vertigo more frequently exhibit a negative otoneurological examination since they are often examined in the intercritical phase. A precise and in-depth research of the patient’s clinical history is the key to suspect or make a diagnosis together with the search for some instrumental or clinical hallmark, especially in cases where the clinical picture does not fully meet the international diagnostic criteria.