bluuebunny/crossref_metadata_2025 huggingface dataset:
Abstract 940: Comprehensive characterization of MET exon 14 skipping mutations in non-small cell lung cancer
Authors: Wu Xue , Zhang Yaru , Pang Jiaohui , Lin Li , Yin Jiani C. , Tang Haimeng , Shao Yang
Published: April 2023

Background: MET exon 14 skipping mutation (METΔex14) is a key driver event in non-small cell lung cancer (NSCLC), and can be acquired following drug resistance to MET, EGFR or ALK inhibitors. Clinical and genomic features of METΔex14 in NSCLC remain to be further characterized.

Methods: A total of 556 baseline samples, 54 MET inhibitor-resistant samples and 16 EGFR/ALK inhibitor-resistant samples from 586 METΔex14 NSCLC patients were included in the analysis. Tissue and/or plasma samples were subjected to targeted next-generation sequencing (NGS) using Geneseeq’s 139/425 cancer-relevant gene panels.

Results: The overall frequency of METΔex14 was 1.1%, with a total of 266 distinct variants detected. Of these, 40.2% were base substitutions in the intronic non-coding region immediately adjacent to the splice acceptor site, followed by indels (25.6%) and base substitutions (4.5%) in the splice donor site, and indels (21.4%) and base substitutions (2.3%) in the splice acceptor site, etc. No associations with clinical features were found among different variants. At baseline, METΔex14 commonly co-occurred with TP53 mutations (40.8%), and CDK4 (15.8%) and MET (7.9%) amplifications. In 42 (7.6%) patients, concurrent oncogenic mutations in EGFR, KRAS, ALK, RET, BRAF, and ERBB2 were also detected. Such patients displayed a different spectrum of METΔex14 variants compared with those without concurrent oncogenic mutations (P=0.08). In patients treated with MET inhibitors, no difference in survival was seen among different METΔex14 variants (P=0.38). Notably, activation of EGFR or MET (kinase domain mutations/amplification) conferred primary resistance to savolitinib and crizotinib. In addition, patients with high chromosomal instability or low intratumoral heterogeneity had unfavorable PFS. Secondary resistance to MET-TKI included MET activation (D1228N/H, Y1230C/S, amplification), EGFR activating mutations/amplification, KRAS G12V, as well as FGFR2/4 amplification. Surprisingly, we identified a FGFR3-TACC3 fusion as a previously unreported secondary resistance mechanism to savolitinib in the second line setting. Finally, we found that MET exon 14 skipping mutation might be a rare secondary resistance mechanism to EGFR/ALK inhibitors.

Conclusion: In this study, we characterized METΔex14 in a large cohort of NSCLC patients and explored potential primary and secondary resistance mechanisms to savolitinib and crizotinib, which may facilitate drug development and help inform clinical actions.

Citation Format: Xue Wu, Yaru Zhang, Jiaohui Pang, Li Lin, Jiani C. Yin, Haimeng Tang, Yang Shao. Comprehensive characterization of MET exon 14 skipping mutations in non-small cell lung cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 940.
Abstract 952: Prioritizing mutations associated with smoking as a variable in lung cancer precision medicine with immunotherapies
Authors: Smith Margaret Rose , Wang Yuezhu , D’Agostino Ralph , Lin Yin , Ruiz Jimmy , Lycan Thomas , Topaloglu Umit , Abdulhaleem Mohammed , Chan Michael , Xing Fei
Published: April 2023

Background: In 2022, 230,000 new lung cancer cases will be diagnosed in the United States. The treatment regimen for non-small cell lung cancer (NSCLC) has drastically changed owing to the superior anti-cancer effects of immunotherapies. Immune checkpoint inhibitors (ICI) and chemo-immunotherapy (chemo-ICI) are the first-line treatments for NSCLC. Tumor Mutation Burden (TMB) and PD-L1 expression in tumor cells are potential biomarkers in predicting a patient’s survival and response to ICI. However, emerging data have shown that TMB and PD-L1 may no longer be an adequate biomarkers in predicting a patient’s response to ICI or chemo-ICI. We hypothesize that by using tumor-sequencing data and taking into effect a patient’s smoking status, we can identify biomarkers that predict survival to either ICI or chemo-ICI.

Methods: To identify biomarkers, we collected genomic sequencing data and comprehensive clinical characteristics on 424 NSCLC patients who received ICI or chemo-ICI treatment at Atrium Health Wake Forest Baptist. Cox-proportional hazard regression models were fit to identify mutations that were “beneficial” (HR < 1) or “detrimental” (HR > 1) for patients on different treatment regimens, followed by the generation of mutation composite scores (MCS) for each treatment. Co-occurrence analysis was performed to identify novel co-occurring and mutually exclusive mutations in each treatment and smoking group by mutation interaction analysis.

Results: We identify beneficial and harmful mutations in patients that received ICI or chemo-ICI treatment. We also identified unique biomarkers based on smoking statues. We then created an MCS for each smoking statues group and treatment type to assist personalize treatment.

Future directions: We will validate these results in other institute cohorts and add other clinical characteristics to personalize treatment based on MCS for an individual patient.

Citation Format: Margaret Rose Smith, Yuezhu Wang, Ralph D’Agostino, Yin Lin, Jimmy Ruiz, Thomas Lycan, Umit Topaloglu, Mohammed Abdulhaleem, Michael Chan, Fei Xing. Prioritizing mutations associated with smoking as a variable in lung cancer precision medicine with immunotherapies [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 952.
Abstract 970: Clinical analysis of patients (pts) with lung cancer treated with anti-PD1/PDL1 therapy
Authors: Zhao Xin , Liu Siyao , Su Junyan
Published: April 2023

Background: Characterization of Lung cancer treated with anti-PD1/PDL1 therapy was approach to predict the clinical benefit. This research is a prospective and observational study of long-term immunization therapy safety in routine clinical practice in lung cancer.

Methods: This study obtained 395 Chinese patients lung cancer patients who underwent surgical resection or biopsies from January 2020 to January 2022. It is aimed to evaluated the value of TMB, MSI, and PD-1 expression in the pts subset that was therapy at anti-PD1/PDL1 therapy(n=47), other anticancer therapies (n=348) included chemoradiation therapy(55%), docetaxel(18%), EGFR-TKIs (16%), pemetrexed (5%) etc.

Results: In this cohort, lung cancer pts treated with anti-PD1/PDL1 therapy had a higher proportion of MSI-H than others (7.7% vs 3.4%). PD-L1 High expression in two groups (anti-PD1/PDL1 therapy vs others) about in 19.1%(n=9) vs 10.6% (n=37), and low expression in 8.9% (n =31) vs 10.6% (n=5) pts. Median TMB was 13 mut/Mb from anti-PD1/PDL1 therapy and 9.6 mut/Mb from other therapy.

Conclusions: Lung cancer pts were integrated after to immunotherapy vs other therapy, most pts received multiple subsequent anticancer therapies. In this study, immune-related biomarker analysis of lung cancer populations with multiple treatment methods may help improve the efficacy of precise treatment in lung cancer.

Table Other therapy(n = 348) Anti-PD1/PDL1 therapy(n = 47) PD-L1 IHC High 10.6% 19.1% PD-L1 IHC Low 8.9% 10.6% TMB Median (mut/Mb) 9.6 13.0 MSS Status 76.0% 74.4% MSI Low Status 20.7% 17.9% MSI High Status 3.4% 7.7%

Citation Format: Xin Zhao, Siyao Liu, Junyan Su. Clinical analysis of patients (pts) with lung cancer treated with anti-PD1/PDL1 therapy [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 970.
Abstract SY31-02: Determinants of hypoxia in pancreatic cancer and identification of ULK1 as a potential therapeutic target
Authors: Zhang Ji , Cojocari Dan , Cairns Rob , Koritzinsky Marianne , Wouters Bradly G.
Published: April 2023

The effectiveness of radiation treatment is impacted by spatially defined features of the tumor microenvironment including areas of tumor hypoxia. This is due in part to direct protection against radiation damage by hypoxia, as well as through the influence of hypoxia on tumor, stroma and immune cell phenotypes. Despite this understanding, our progress in advancing therapies to address tumor hypoxia in patients have been largely unsuccessful. This is driven in part to the incredible variability in tumor hypoxia across patients as well as to a limited understanding of the underlying causes and consequences of hypoxia on an individual patient basis. As a step towards more precision approaches for hypoxia directed therapies we are characterizing hypoxia and its causes in individual patients and in matched patient derived xenografts. Our data reveal a striking variation in hypoxia across patients, and a corresponding variation in both oxygen consumption and hypoxia tolerance in patient derived organoids. We hypothesize that levels of hypoxia within individual tumors are determined through separate processes that drive changes in oxygen demand coupled with those that influence adaptation and survival to hypoxic stress. We have also identified ULK1 as a key contributor to both of these processes. Loss or inhibition of ULK1, a gene activated downstream of the unfolded protein response during hypoxia, results in defects in both mitochondrial and ER turnover leading to increases in both oxygen consumption and ER stress. Consequently, knockdown of ULK1 in established pancreatic cancer xenografts results in development of more hypoxia, while at the same time promotes the death of cells that become hypoxic. This has a dramatic impact on tumor growth, leading to complete remission in some models and suggesting that ULK1 should be pursued as a tumor microenvironment therapeutic target.

Citation Format: Ji Zhang, Dan Cojocari, Rob Cairns, Marianne Koritzinsky, Bradly G. Wouters. Determinants of hypoxia in pancreatic cancer and identification of ULK1 as a potential therapeutic target. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr SY31-02.
Supplementary Figure Legend from Inhibition of mTOR Pathway Sensitizes Acute Myeloid Leukemia Cells to Aurora Inhibitors by Suppression of Glycolytic Metabolism
Authors: Liu Ling-Ling , Long Zi-Jie , Wang Le-Xun , Zheng Fei-Meng , Fang Zhi-Gang , Yan Min , Xu Dong-Fan , Chen Jia-Jie , Wang Shao-Wu , Lin Dong-Jun , Liu Quentin
Published: April 2023

PDF file - 82K
Supplementary Figure 7 from Inhibition of mTOR Pathway Sensitizes Acute Myeloid Leukemia Cells to Aurora Inhibitors by Suppression of Glycolytic Metabolism
Authors: Liu Ling-Ling , Long Zi-Jie , Wang Le-Xun , Zheng Fei-Meng , Fang Zhi-Gang , Yan Min , Xu Dong-Fan , Chen Jia-Jie , Wang Shao-Wu , Lin Dong-Jun , Liu Quentin
Published: April 2023

PDF file - 1715K, Aurora kinases inhibitors and mTOR inhibitors had synergistic effect on the expression of cleaved caspase 3 and p62.
Supplementary Figure 5 from Inhibition of mTOR Pathway Sensitizes Acute Myeloid Leukemia Cells to Aurora Inhibitors by Suppression of Glycolytic Metabolism
Authors: Liu Ling-Ling , Long Zi-Jie , Wang Le-Xun , Zheng Fei-Meng , Fang Zhi-Gang , Yan Min , Xu Dong-Fan , Chen Jia-Jie , Wang Shao-Wu , Lin Dong-Jun , Liu Quentin
Published: April 2023

PDF file - 2735K, Aurora kinases inhibitors had synergistic effect with mTOR inhibitors on the proliferation of U937 and NB4 cells.
Supplementary Figure Legend from Activation of the Wnt Pathway through AR79, a GSK3β Inhibitor, Promotes Prostate Cancer Growth in Soft Tissue and Bone
Authors: Jiang Yuan , Dai Jinlu , Zhang Honglai , Sottnik Joe L. , Keller Jill M. , Escott Katherine J. , Sanganee Hitesh J. , Yao Zhi , McCauley Laurie K. , Keller Evan T.
Published: April 2023

PDF file - 56K
Supplementary Table 5 from RAS/MEK–Independent Gene Expression Reveals BMP2-Related Malignant Phenotypes in the Nf1-Deficient MPNST
Authors: Sun Daochun , Haddad Ramsi , Kraniak Janice M. , Horne Steven D. , Tainsky Michael A.
Published: April 2023

XLSX file - 12K, Primers for qPCR verification
Supplementary Table 2 from RAS/MEK–Independent Gene Expression Reveals BMP2-Related Malignant Phenotypes in the Nf1-Deficient MPNST
Authors: Sun Daochun , Haddad Ramsi , Kraniak Janice M. , Horne Steven D. , Tainsky Michael A.
Published: April 2023

XLSX file - 810K, Data Set 1-10
Supplementary Table 1 from RAS/MEK–Independent Gene Expression Reveals BMP2-Related Malignant Phenotypes in the Nf1-Deficient MPNST
Authors: Sun Daochun , Haddad Ramsi , Kraniak Janice M. , Horne Steven D. , Tainsky Michael A.
Published: April 2023

XLSX file - 11K, Differently expressed genes in each comparison scenario
Supplementary Figure 4 from Fer Protein-Tyrosine Kinase Promotes Lung Adenocarcinoma Cell Invasion and Tumor Metastasis
Authors: Ahn Joseph , Truesdell Peter , Meens Jalna , Kadish Carli , Yang Xiaolong , Boag Alexander H. , Craig Andrew W.B.
Published: April 2023

PDF file - 120K, Fer promotes migration and invasion of A549 cells.
Supplementary Figure 3 from Interleukin-6 and Oncostatin-M Synergize with the PI3K/AKT Pathway to Promote Aggressive Prostate Malignancy in Mouse and Human Tissues
Authors: Smith Daniel A. , Kiba Atsushi , Zong Yang , Witte Owen N.
Published: April 2023

PDF file - 5091K, Expression of IL6 or OSM in the context of PTEN loss drive malignant invasion of epithelial cells into the surrounding mesenchyme.
Supplementary Table 1 from Systems Analysis of the NCI-60 Cancer Cell Lines by Alignment of Protein Pathway Activation Modules with “-OMIC” Data Fields and Therapeutic Response Signatures
Authors: Federici Giulia , Gao Xi , Slawek Janusz , Arodz Tomasz , Shitaye Amanuel , Wulfkuhle Julia D. , De Maria Ruggero , Liotta Lance A. , Petricoin Emanuel F.
Published: April 2023

PDF file - 107K, List of antibodies against total-, phospho- and cleaved-proteins, with dilutions used for RPPA analysis.
Supplementary Figure 3 from Systems Analysis of the NCI-60 Cancer Cell Lines by Alignment of Protein Pathway Activation Modules with “-OMIC” Data Fields and Therapeutic Response Signatures
Authors: Federici Giulia , Gao Xi , Slawek Janusz , Arodz Tomasz , Shitaye Amanuel , Wulfkuhle Julia D. , De Maria Ruggero , Liotta Lance A. , Petricoin Emanuel F.
Published: April 2023

PDF file - 142K, Unsupervised hierarchical clustering representing the NCI-60 cell lines (horizontal axis) and the calculated six pathway activation module scores (vertical axis): IGF1-R, apoptosis, integrin, mTOR, AKT and EGFR. The NCI-60 cell lines have been colorized according to their tissue origin: orange - kidney, blue - lung, pink - breast, red - blood, green - colon, brown - prostate, purple - ovary, black - skin and gray - central nervous system.
Supplementary Figure 1 from Nullifying the CDKN2AB Locus Promotes Mutant K-ras Lung Tumorigenesis
Authors: Schuster Katja , Venkateswaran Niranjan , Rabellino Andrea , Girard Luc , Peña-Llopis Samuel , Scaglioni Pier Paolo
Published: April 2023

PDF file - 2662K, Tumor burden in LSL Kras mice.
Supplementary Fig. 8 from Transcription Factor Interactions Mediate EGF-Dependent COX-2 Expression
Authors: Xu Kaiming , Shu Hui-Kuo G.
Published: April 2023

Supplementary Fig. 8 - PDF file 207K, FOXM1 overexpression induces COX-2 levels independent of Sp1
Supplementary Fig. 5 from Transcription Factor Interactions Mediate EGF-Dependent COX-2 Expression
Authors: Xu Kaiming , Shu Hui-Kuo G.
Published: April 2023

Supplementary Fig. 5 - PDF file 146K, PKC-delta and Sp1 are downstream of Src signaling
Supplementary Table 2 from Loss of a Negative Feedback Loop Involving Pea3 and Cyclin D2 Is Required for Pea3-Induced Migration in Transformed Mammary Epithelial Cells
Authors: Ladam Franck , Damour Isabelle , Dumont Patrick , Kherrouche Zoulika , de Launoit Yvan , Tulasne David , Chotteau-Lelievre Anne
Published: April 2023

PDF file - 69K, Primers sets used for Q-PCR and ChIP experiments.
Supplementary Figure 1 from Loss of a Negative Feedback Loop Involving Pea3 and Cyclin D2 Is Required for Pea3-Induced Migration in Transformed Mammary Epithelial Cells
Authors: Ladam Franck , Damour Isabelle , Dumont Patrick , Kherrouche Zoulika , de Launoit Yvan , Tulasne David , Chotteau-Lelievre Anne
Published: April 2023

PDF file - 270K, Relative pea3 mRNA and protein expression in TAC-V vs TAC-Pea3-V. Wound healing assay for TAC-V and TAC-Pea3-V cells treated or not with TGF-β1 and with or without Mitomycin C. Apoptosis measurement.
Supplementary Figure 5 from Oncogenic MUC1-C Promotes Tamoxifen Resistance in Human Breast Cancer
Authors: Kharbanda Akriti , Rajabi Hasan , Jin Caining , Raina Deepak , Kufe Donald
Published: April 2023

PDF file - 622K, BT474/MUC1shRNA (A) and MCF-7/vector (B) cells were treated with fixed IC50 ratios of (i) GO-203 alone on days 0, 1, 2, 3 and 4, (ii) tamoxifen alone on days 0, 2 and 4 and (iii) the GO-203/tamoxifen combination. For tamoxifen-resistant BT-474/CshRNA and MCF-7/MUC1-C cells, tamoxifen was used at the half-maximal inhibitory concentrations obtained for the tamoxifen-sensitive BT-474/MUC1shRNA and MCF-7/vector cells, respectively. The multiple effect-level isobologram analyses are shown for the ED50 (X), ED75 (+) and ED90 (⊗) values.
Supplementary Figure 4 from Oncogenic MUC1-C Promotes Tamoxifen Resistance in Human Breast Cancer
Authors: Kharbanda Akriti , Rajabi Hasan , Jin Caining , Raina Deepak , Kufe Donald
Published: April 2023

PDF file - 338K, 293T cells were left untreated or treated with G0-203 each day for 4 days. The results (mean plus-minus SD of three replicates) are expressed as the percentage of cell death as determined by trypan blue staining on day 4.
Supplementary Figure 3 from Oncogenic MUC1-C Promotes Tamoxifen Resistance in Human Breast Cancer
Authors: Kharbanda Akriti , Rajabi Hasan , Jin Caining , Raina Deepak , Kufe Donald
Published: April 2023

PDF file - 601K, MCF-7/vector and MCF-7/MUC1-C cells were left untreated or treated with 5 μM tamoxifen for 2 days. Lysates were immunoblotted with the indicated antibodies.
Supplementary Figure 6 from Resveratrol and P-glycoprotein Inhibitors Enhance the Anti-Skin Cancer Effects of Ursolic Acid
Authors: Junco Jacob J. , Mancha Anna , Malik Gunjan , Wei Sung-Jen , Kim Dae Joon , Liang Huiyun , Slaga Thomas J.
Published: April 2023

PDF file - 2350K, Figure S6: Relative effect of vehicles on Rh123 levels in MT1/2 vs Ca3/7 cells (average of 3 independent experiments, n=3/exp, * p<0.05, ** p<0.01, *** p<0.005, and **** p<0.001).
Supplementary Figure 5 from Resveratrol and P-glycoprotein Inhibitors Enhance the Anti-Skin Cancer Effects of Ursolic Acid
Authors: Junco Jacob J. , Mancha Anna , Malik Gunjan , Wei Sung-Jen , Kim Dae Joon , Liang Huiyun , Slaga Thomas J.
Published: April 2023

PDF file - 955K, Figure S5: UA and RES synergistically reduced viability of human lung adenocarcinoma cell line A549 (one experiment, n=3, * indicates p<0.05 between UA+veh and UA+10microM RES, # indicates p<0.05 between UA+veh and UA+25microM RES).
Supplementary Table 1 from Autophagy-Dependent Metabolic Reprogramming Sensitizes TSC2-Deficient Cells to the Antimetabolite 6-Aminonicotinamide
Authors: Parkhitko Andrey A. , Priolo Carmen , Coloff Jonathan L. , Yun Jihye , Wu Julia J. , Mizumura Kenji , Xu Wenping , Malinowska Izabela A. , Yu Jane , Kwiatkowski David J. , Locasale Jason W. , Asara John M. , Choi Augustine M.K. , Finkel Toren , Henske Elizabeth P.
Published: April 2023

XLSX file - 144K, Metabolomics data (raw and processed).
Supplementary Figure Legend from Mitoxantrone Targets Human Ubiquitin-Specific Peptidase 11 (USP11) and Is a Potent Inhibitor of Pancreatic Cancer Cell Survival
Authors: Burkhart Richard A. , Peng Yu , Norris Zoë A. , Tholey Renée M. , Talbott Vanessa A. , Liang Qin , Ai Yongxing , Miller Kathy , Lal Shruti , Cozzitorto Joseph A. , Witkiewicz Agnieska K. , Yeo Charles J. , Gehrmann Matthew , Napper Andrew , Winter Jordan M. , Sawicki Janet A. , Zhuang Zhihao , Brody Jonathan R.
Published: April 2023

PDF file - 59K
Supplementary Figure 1 from Mitoxantrone Targets Human Ubiquitin-Specific Peptidase 11 (USP11) and Is a Potent Inhibitor of Pancreatic Cancer Cell Survival
Authors: Burkhart Richard A. , Peng Yu , Norris Zoë A. , Tholey Renée M. , Talbott Vanessa A. , Liang Qin , Ai Yongxing , Miller Kathy , Lal Shruti , Cozzitorto Joseph A. , Witkiewicz Agnieska K. , Yeo Charles J. , Gehrmann Matthew , Napper Andrew , Winter Jordan M. , Sawicki Janet A. , Zhuang Zhihao , Brody Jonathan R.
Published: April 2023

PDF file - 28K, S1. Cell growth in a high USP11 expression cell line (PL5) following siRNA treatment targeting USP11.
Supplementary Figure 5 from PHD4 Stimulates Tumor Angiogenesis in Osteosarcoma Cells via TGF-α
Authors: Klotzsche-von Ameln Anne , Prade Ina , Grosser Marianne , Kettelhake Antje , Rezaei Maryam , Chavakis Triantafyllos , Flamme Ingo , Wielockx Ben , Breier Georg
Published: April 2023

PDF file - 133K, S5. Lentivirus-mediated silencing of HIF-2alpha in LM8 control cells.
Supplementary Table 2 from The Twist Box Domain Is Required for Twist1-induced Prostate Cancer Metastasis
Authors: Gajula Rajendra P. , Chettiar Sivarajan T. , Williams Russell D. , Thiyagarajan Saravanan , Kato Yoshinori , Aziz Khaled , Wang Ruoqi , Gandhi Nishant , Wild Aaron T. , Vesuna Farhad , Ma Jinfang , Salih Tarek , Cades Jessica , Fertig Elana , Biswal Shyam , Burns Timothy F. , Chung Christine H. , Rudin Charles M. , Herman Joseph M. , Hales Russell K. , Raman Venu , An Steven S. , Tran Phuoc T.
Published: April 2023

PDF file - 116K, Supplemental Table S2 - Myc-CaP + Twist1-F191G versus Myc-CaP + Vector Differentially.
Supplementary Table 1 from The Twist Box Domain Is Required for Twist1-induced Prostate Cancer Metastasis
Authors: Gajula Rajendra P. , Chettiar Sivarajan T. , Williams Russell D. , Thiyagarajan Saravanan , Kato Yoshinori , Aziz Khaled , Wang Ruoqi , Gandhi Nishant , Wild Aaron T. , Vesuna Farhad , Ma Jinfang , Salih Tarek , Cades Jessica , Fertig Elana , Biswal Shyam , Burns Timothy F. , Chung Christine H. , Rudin Charles M. , Herman Joseph M. , Hales Russell K. , Raman Venu , An Steven S. , Tran Phuoc T.
Published: April 2023

PDF file - 384K, Supplemental Table S1 - Myc-CaP + Twist1 versus Myc-CaP + Vector Differentially.
Supplementary Methods from The Impact of miRNA-Based Molecular Diagnostics and Treatment of NRF2-Stabilized Tumors
Authors: Yamamoto Shinsuke , Inoue Jun , Kawano Tatsuyuki , Kozaki Ken-ichi , Omura Ken , Inazawa Johji
Published: April 2023

PDF file - 112K
Supplementary Figure 7 from The Impact of miRNA-Based Molecular Diagnostics and Treatment of NRF2-Stabilized Tumors
Authors: Yamamoto Shinsuke , Inoue Jun , Kawano Tatsuyuki , Kozaki Ken-ichi , Omura Ken , Inazawa Johji
Published: April 2023

PDF file - 94K, Expression analysis of the four miRNAs and the effect of transfection of miR-507 on cell survival under cellular stress caused by Cisplatin (CDDP) treatment (left) in A549 cells.
Supplementary Figure Legend and Tables 1 - 2 from Molecular Dissection of AKT Activation in Lung Cancer Cell Lines
Authors: Guo Yanan , Du Jinyan , Kwiatkowski David J.
Published: April 2023

PDF file - 103K, Supplemental Table 1. Cell lines assessed for persistent AKT-S473 phosphorylation in the absence of serum. Supplemental Table 2. Tyrosine kinase phosphorylation profiling.
Supplementary Figure 4 from Molecular Dissection of AKT Activation in Lung Cancer Cell Lines
Authors: Guo Yanan , Du Jinyan , Kwiatkowski David J.
Published: April 2023

PDF file - 2483K, Synergistic effects of mTOR kinase and rapalogue therapy with Dasatanib on HCC2429 cells.
Supplementary Figure 1 from Molecular Dissection of AKT Activation in Lung Cancer Cell Lines
Authors: Guo Yanan , Du Jinyan , Kwiatkowski David J.
Published: April 2023

PDF file - 1285K, HER2 amplification and Afatinib sensitivity in H1819 and Calu-3.
Supplementary Figure Legend from RASEF is a Novel Diagnostic Biomarker and a Therapeutic Target for Lung Cancer
Authors: Oshita Hideto , Nishino Ryohei , Takano Atsushi , Fujitomo Takashi , Aragaki Masato , Kato Tatsuya , Akiyama Hirohiko , Tsuchiya Eiju , Kohno Nobuoki , Nakamura Yusuke , Daigo Yataro
Published: April 2023

PDF file - 61K
Supplementary Figure 2 from Activation of the NF-κB Pathway by the STAT3 Inhibitor JSI-124 in Human Glioblastoma Cells
Authors: McFarland Braden C. , Gray G. Kenneth , Nozell Susan E. , Hong Suk W. , Benveniste Etty N.
Published: April 2023

PDF file - 86K, JSI-124 induces the mRNA expression of inflammatory genes in human GBM cells.
Supplementary Figure 1 from Activation of the NF-κB Pathway by the STAT3 Inhibitor JSI-124 in Human Glioblastoma Cells
Authors: McFarland Braden C. , Gray G. Kenneth , Nozell Susan E. , Hong Suk W. , Benveniste Etty N.
Published: April 2023

PDF file - 135K, JSI-124 inhibits stimulus-induced and constitutive JAK2/STAT3 phosphorylation and inhibits proliferation in GBM cells.
Supplementary Figure 4 from HER-2/neu Mediates Oncogenic Transformation via Altered CREB Expression and Function
Authors: Steven André , Leisz Sandra , Massa Chiara , Iezzi Manuela , Lattanzio Rossano , Lamolinara Alessia , Bukur Jürgen , Müller Anja , Hiebl Bernhard , Holzhausen Hans-Jürgen , Seliger Barbara
Published: April 2023

PDF file - 8658K, Immunohistochemical analysis of HER-2/neu+ cells and shCREB-HER-2/neu+ cells following in-vivo tumorigenicity. (A) Representative images from organs of tumor-bearing mice injected with HER-2/neu+ cells and shCREB-HER-2/neu+ cells are shown. The tumors were fixed, embedded in paraffin, and 5 �m slices were stained as indicated. Magnification, 20�. (B) Tumor slices were stained with hematoxylin-eosin and analyzed for apoptosis (TUNEL), proliferation (Ki-67) and CREB expression. Following incubation with a horse-radish-peroxidase-linked antibody, the slices were stained with diaminobenzidine and counterstained with methylene blue. Arrows (apoptosis staining), blood vessels; arrowheads, apoptotic cells. Magnification, 20�.
Supplementary Table 2 from Downregulation of HOPX Controls Metastatic Behavior in Sarcoma Cells and Identifies Genes Associated with Metastasis
Authors: Kovářová Denisa , Plachý Jiří , Kosla Jan , Trejbalová Kateřina , Čermák Vladimír , Hejnar Jiří
Published: April 2023

XLS file, 482K, List of differentially expressed genes with at least two-fold change.
Supplementary Tables 1-2 from T-Type Ca2+ Channel Inhibition Induces p53-Dependent Cell Growth Arrest and Apoptosis through Activation of p38-MAPK in Colon Cancer Cells
Authors: Dziegielewska Barbara , Brautigan David L. , Larner James M. , Dziegielewski Jaroslaw
Published: April 2023

PDF file - 19 KB, Supplemental Table 1. Sequence of primers designed for RT-qPCR amplification of p53 regulated genes. Supplementary Table 2. Sequence of primers designed for amplification of genomic DNA of p53 regulated genes containing or in close proximity to p53BS.
Supplementary Figure 1 from EGFR Inhibition Induces Proinflammatory Cytokines via NOX4 in HNSCC
Authors: Fletcher Elise V.M. , Love-Homan Laurie , Sobhakumari Arya , Feddersen Charlotte R. , Koch Adam T. , Goel Apollina , Simons Andrean L.
Published: April 2023

PDF file - 69K, Erlotinib induced IL-6 protein expression.
Supplementary Table 1 from PGE2-Driven Expression of c-Myc and OncomiR-17-92 Contributes to Apoptosis Resistance in NSCLC
Authors: Krysan Kostyantyn , Kusko Rebecca , Grogan Tristan , O'Hearn James , Reckamp Karen L. , Walser Tonya C. , Garon Edward B. , Lenburg Marc E. , Sharma Sherven , Spira Avrum E. , Elashoff David , Dubinett Steven M.
Published: April 2023

PDF file - 47K, Patient characteristics.
Supplementary Figure 4 from Inhibition of Hedgehog and Androgen Receptor Signaling Pathways Produced Synergistic Suppression of Castration-Resistant Prostate Cancer Progression
Authors: Gowda Pramod S. , Deng Jianhong D. , Mishra Sweta , Bandyopadhyay Abhik , Liang Sitai , Lin Shu , Mahalingam Devalingam , Sun Lu-Zhe
Published: April 2023

PDF file - 994K, Cyclopamine inhibits Hh signaling activity in AI cells in a dose dependent manner.
Supplementary Figure 3 from Towards Sustained Silencing of HER2/neu in Cancer By Epigenetic Editing
Authors: Falahi Fahimeh , Huisman Christian , Kazemier Hinke G. , van der Vlies Pieter , Kok Klaas , Hospers Geke A.P. , Rots Marianne G.
Published: April 2023

PDF file - 60K, Induction of H3K9me3 in region B of Her2/neu gene in SKBR3 cells.
Supplementary Figure 2 from Towards Sustained Silencing of HER2/neu in Cancer By Epigenetic Editing
Authors: Falahi Fahimeh , Huisman Christian , Kazemier Hinke G. , van der Vlies Pieter , Kok Klaas , Hospers Geke A.P. , Rots Marianne G.
Published: April 2023

PDF file - 77K, Specific binding of HER2/neu ZFP (E2C).
Table S6 from Mastermind-Like 3 Controls Proliferation and Differentiation in Neuroblastoma
Authors: Heynen Guus J.J.E. , Nevedomskaya Ekaterina , Palit Sander , Jagalur Basheer Noorjahan , Lieftink Cor , Schlicker Andreas , Zwart Wilbert , Bernards Rene , Bajpe Prashanth Kumar
Published: April 2023

Supplementary Table 6: RNA sequencing read depths for SK-N-SH and SD3.23 cells.
Supplementary Tables 1 and 2 and Supplementary Figures 1 and 2 from Androgen Receptor Promotes the Oncogenic Function of Overexpressed Jagged1 in Prostate Cancer by Enhancing Cyclin B1 Expression via Akt Phosphorylation
Authors: Yu Yongjiang , Zhang Yu , Guan Wenbin , Huang Tao , Kang Jian , Sheng Xujun , Qi Jun
Published: April 2023

PDF - 184K, Supplementary Table 1. Oligoes used in the manuscript. Supplementary Table 2. Antibodies used in the manuscript. Supplementary Figure 1. Cell cycle and cell cycle genes affected by the Jagged1 and AR. Supplementary Figure 2. Expressions of PSA, KLK2 and TMPRSS2 in LNCaP and LAPC4 cells upon Jagged1 depletion and AKT inactivation (MK-2206 treatment) were detected by RT-PCR.
Table S4 from Mastermind-Like 3 Controls Proliferation and Differentiation in Neuroblastoma
Authors: Heynen Guus J.J.E. , Nevedomskaya Ekaterina , Palit Sander , Jagalur Basheer Noorjahan , Lieftink Cor , Schlicker Andreas , Zwart Wilbert , Bernards Rene , Bajpe Prashanth Kumar
Published: April 2023

Supplementary Table 4: Read counts, number of mapped reads and number of peaks for the RARα and MAML3 ChI
Supplementary Table S1 from Synergistic Loss of Prostate Cancer Cell Viability by Coinhibition of HDAC and PARP
Authors: Chao Olivia S. , Goodman Oscar B.
Published: April 2023

Combination index derived from clonogenic assay of prostate cancer cells treated with SAHA and olaparib.
Supplemental File 3 from Hypoxia Regulates Alternative Splicing of HIF and non-HIF Target Genes
Authors: Sena Johnny A. , Wang Liyi , Heasley Lynn E. , Hu Cheng-Jun
Published: April 2023

Supplemental File 3: primer sequence
Supplementary Table, Figures and Legends from CITED2 Modulates Breast Cancer Metastatic Ability through Effects on IKKα
Authors: Jayaraman Swaathi , Doucet Michele , Lau Wen Min , Kominsky Scott L.
Published: April 2023

cDNA microarray analysis of gene expression between shscramble and shCITED2-expressing MDA-MB-231 cells. Supplementary Fig. S1: Effect of CITED2 knockdown on metastasis to non-bone sites. Supplementary Fig. S2: Regulation of OPN, MMP9, uPA, SPARC, IL-11, and IL-1β by NF-κB signaling in MDA-MB-231 cells. Supplementary Fig. S3: Effect of IKKα expression on breast cancer patient survival.
Supplemental Table S1-5 from Dissecting LncRNA Roles in Renal Cell Carcinoma Metastasis and Characterizing Genomic Heterogeneity by Single-Cell RNA-seq
Authors: Li Xue , Meng Xianwen , Wei Cong , Zhou Yincong , Chen Hongjun , Huang He , Chen Ming
Published: April 2023

Table S1. Information of RNA-seq samples. Table S2. Metastatic vs Primary differentially expressed lncRNAs and PCGs. Table S3. Correlation between CMAL-clusters and clinicopathological features. Table S4. CMALs significantly associated with TNF signaling pathway. Table S5. The qRT-PCR primer sequences of four CMALs and two PCGs.
Supplementary Fig. S3 from CITED2 Modulates Breast Cancer Metastatic Ability through Effects on IKKα
Authors: Jayaraman Swaathi , Doucet Michele , Lau Wen Min , Kominsky Scott L.
Published: April 2023

Effect of IKKα expression on breast cancer patient survival.
Figure S7 from Dissecting LncRNA Roles in Renal Cell Carcinoma Metastasis and Characterizing Genomic Heterogeneity by Single-Cell RNA-seq
Authors: Li Xue , Meng Xianwen , Wei Cong , Zhou Yincong , Chen Hongjun , Huang He , Chen Ming
Published: April 2023

Figure S7. Similarity and variation of lncRNA profiles in single cells.
Supplementary Figure Legend, Tables 1 - 2 from miR-338-3p Suppresses Gastric Cancer Progression through a PTEN-AKT Axis by Targeting P-REX2a
Authors: Guo Bo , Liu Liying , Yao Jiayi , Ma Ruili , Chang Dongmin , Li Zongfang , Song Tusheng , Huang Chen
Published: April 2023

PDF file - 36K, Table S1. Primers for PCR of miRNAs and mRNA. Table S2. Sequence information.
Supplementary Figure 1 from miR-338-3p Suppresses Gastric Cancer Progression through a PTEN-AKT Axis by Targeting P-REX2a
Authors: Guo Bo , Liu Liying , Yao Jiayi , Ma Ruili , Chang Dongmin , Li Zongfang , Song Tusheng , Huang Chen
Published: April 2023

PDF file - 1425K, Figure S1. miR-338-3p is re-expression in BGC823 cells after transfection with pre-miR-338-3p vector.
S4. Colocalization of UCP1 with aggressive markers from Increased Expression of Beige/Brown Adipose Markers from Host and Breast Cancer Cells Influence Xenograft Formation in Mice
Authors: Singh Rajan , Parveen Meher , Basgen John M. , Fazel Sayeda , Meshesha Meron F. , Thames Easter C. , Moore Brandis , Martinez Luis , Howard Carolyn B. , Vergnes Laurent , Reue Karen , Pervin Shehla
Published: April 2023

Sections of xenografts were analyzed for co-expression of collagen and adiponectin.
S2. Validation of species specific primers from Increased Expression of Beige/Brown Adipose Markers from Host and Breast Cancer Cells Influence Xenograft Formation in Mice
Authors: Singh Rajan , Parveen Meher , Basgen John M. , Fazel Sayeda , Meshesha Meron F. , Thames Easter C. , Moore Brandis , Martinez Luis , Howard Carolyn B. , Vergnes Laurent , Reue Karen , Pervin Shehla
Published: April 2023

Human specific and mouse specific primers were validated using human pheochromocytoma-derived peri-adrenal fat and mouse brown adipose tissue respectively.
Supplementary Data from Tumor Cell-Derived Periostin Regulates Cytokines That Maintain Breast Cancer Stem Cells
Authors: Lambert Arthur W. , Wong Chen Khuan , Ozturk Sait , Papageorgis Panagiotis , Raghunathan Rekha , Alekseyev Yuriy , Gower Adam C. , Reinhard Björn M. , Abdolmaleky Hamid M. , Thiagalingam Sam
Published: April 2023

This file contains a list of supplementary data and supplementary materials and methods.
Figure S3 from Linking Cancer Metabolism to DNA Repair and Accelerated Senescence
Authors: Efimova Elena V. , Takahashi Satoe , Shamsi Noumaan A. , Wu Ding , Labay Edwardine , Ulanovskaya Olesya A. , Weichselbaum Ralph R. , Kozmin Sergey A. , Kron Stephen J.
Published: April 2023

IRIF persistence induced by glucose-limiting condition or 2-DG is PARP independent.
Supplementary Figure 5 from Taxol Induces Brk-dependent Prosurvival Phenotypes in TNBC Cells through an AhR/GR/HIF–driven Signaling Axis
Authors: Regan Anderson Tarah M. , Ma Shihong , Perez Kerkvliet Carlos , Peng Yan , Helle Taylor M. , Krutilina Raisa I. , Raj Ganesh V. , Cidlowski John A. , Ostrander Julie H. , Schwertfeger Kathryn L. , Seagroves Tiffany N. , Lange Carol A.
Published: April 2023

Confirmation of HIF knockout in MDA-MB-231 cells.
Figure S2 from Linking Cancer Metabolism to DNA Repair and Accelerated Senescence
Authors: Efimova Elena V. , Takahashi Satoe , Shamsi Noumaan A. , Wu Ding , Labay Edwardine , Ulanovskaya Olesya A. , Weichselbaum Ralph R. , Kozmin Sergey A. , Kron Stephen J.
Published: April 2023

Targeting glycolysis blocks IRIF resolution.
Supplementary Figure 1 from Taxol Induces Brk-dependent Prosurvival Phenotypes in TNBC Cells through an AhR/GR/HIF–driven Signaling Axis
Authors: Regan Anderson Tarah M. , Ma Shihong , Perez Kerkvliet Carlos , Peng Yan , Helle Taylor M. , Krutilina Raisa I. , Raj Ganesh V. , Cidlowski John A. , Ostrander Julie H. , Schwertfeger Kathryn L. , Seagroves Tiffany N. , Lange Carol A.
Published: April 2023

Western blot and qPCR data of C57bl/6 mice treated with vehicle or dexamethasone for 6 hours, demonstrating increased Sik expression upon dex treatment.
Supplementary Figure 2 from Constitutively Active ErbB2 Regulates Cisplatin-Induced Cell Death in Breast Cancer Cells via Pro- and Antiapoptotic Mechanisms
Authors: Sigurðsson Haraldur H. , Olesen Christina W. , Dybboe Rie , Lauritzen Gitte , Pedersen Stine F.
Published: April 2023

Supplementary figure 2: Inhibition of ATM and knockdown of noxa, caspase-9, Î¼-calpain and cathepsin B. MCF-7 vector (V) and Î”NErbB2 (Î”N) cells were washed free of tetracycline 72 h before the experiment. Cells were exposed to KU-55933 (10 Î¼M) for 19 h, cisplatin (25 Î¼M) or control conditions for 18 h and subsequently processed for immunoblotting (A-B). Top panels show representative immunoblots, lower panels show protein level relative to that in vector cells under control conditions. A. p-ATM B. p-p53. Î”NErbB2 cells were washed and transfected with siRNA (200nM) 72 h before the experiment. Cells were exposed to cisplatin (25 Î¼M), and subsequently processed for immunoblotting (C-F). Top panels show representative immunoblots, lower panels show protein level relative to that in cells transfected with mock control. C. Noxa D. Caspase-9. E. Î¼-calpain. F. Cathepsin B. Data are representative of 3-4 independent experiments for each condition. Western blot data are shown as means with SEM error bars. * indicates comparison of the value in cisplatin treated to that in the respective cell line control, # indicate comparison of values in vector- and Î”NErbB2 cell lines under same treatment. */#, **/##, ***/### and ****/#### indicate p < 0.05, p < 0.01, p < 0.001 and p < 0.0001, respectively (C-F), one-way ANOVA with Bonferroni post-test) (A-B, two-way ANOVA with Bonferroni post-test).
Figure S3 from Differential Expression of OATP1B3 Mediates Unconjugated Testosterone Influx
Authors: Sissung Tristan M. , Ley Ariel M. , Strope Jonathan D. , McCrea Edel M. , Beedie Shaunna , Peer Cody J. , Shukla Suneet , van Velkinburgh Jennifer , Reece Kelie , Troutman Sarah , Campbell Tessa , Fernandez Elena , Huang Phoebe , Smith Jordan , Thakkar Nilay , Venzon David J. , Brenner Stefan , Lee Wooin , Merino Maria , Luo Ji , Jager Walter , Price Douglas K. , Chau Cindy H. , Figg William D.
Published: April 2023

S3. t-SLCO1B3 expression was optimized using various concentrations of chetomin at different timepoints in (A) LNCaP and (B) 22Rv1 cell lines.
Figure S1 from p53 Activity Dominates That of p73 upon Mdm4 Loss in Development and Tumorigenesis
Authors: Tashakori Mehrnoosh , Zhang Yun , Xiong Shunbin , You M. James , Lozano Guillermina
Published: April 2023

Representative pictures of the gross morphology of Mdm4Î”2/Î”2 p53+â�„- embryos.
Supplementary Table S1 from Adipocytes Sequester and Metabolize the Chemotherapeutic Daunorubicin
Authors: Sheng Xia , Parmentier Jean-Hugues , Tucci Jonathan , Pei Hua , Cortez-Toledo Omar , Dieli-Conwright Christina M. , Oberley Matthew J. , Neely Michael , Orgel Etan , Louie Stan G. , Mittelman Steven D.
Published: April 2023

Primer sequences used for rtPCR.
Supplementary Table 1. Detected RTK alterations and MAP2K1 mutations in sequenced colorectal carcinomas. from Identification of Targetable Kinase Alterations in Patients with Colorectal Carcinoma That are Preferentially Associated with Wild-Type RAS/RAF
Authors: Hechtman Jaclyn F. , Zehir Ahmet , Yaeger Rona , Wang Lu , Middha Sumit , Zheng Tao , Hyman David M. , Solit David , Arcila Maria E. , Borsu Laetitia , Shia Jinru , Vakiani Efsevia , Saltz Leonard , Ladanyi Marc
Published: April 2023

Detected RTK alterations and MAP2K1 mutations in sequenced colorectal carcinomas.
Supplemental Table 1 from Hypoxia Selectively Enhances Integrin α5β1 Receptor Expression in Breast Cancer to Promote Metastasis
Authors: Ju Julia A. , Godet Inês , Ye I Chae , Byun Jungmin , Jayatilaka Hasini , Lee Sun Joo , Xiang Lisha , Samanta Debangshu , Lee Meng Horng , Wu Pei-Hsun , Wirtz Denis , Semenza Gregg L. , Gilkes Daniele M.
Published: April 2023

Supplemental Table 1 - Primer Sequences used for Real-Time qPCR
Supplementary Table 6 from Differential Expression of Stress and Immune Response Pathway Transcripts and miRNAs in Normal Human Endothelial Cells Subjected to Fractionated or Single-Dose Radiation
Authors: Palayoor Sanjeewani T. , John-Aryankalayil Molykutty , Makinde Adeola Y. , Falduto Michael T. , Magnuson Scott R. , Coleman C. Norman
Published: April 2023

PDF - 34K, Changes in selected differentially expressed genes identified by microarray analysis were confirmed by real-time RT-PCR.
Supplementary Table 4 from Differential Expression of Stress and Immune Response Pathway Transcripts and miRNAs in Normal Human Endothelial Cells Subjected to Fractionated or Single-Dose Radiation
Authors: Palayoor Sanjeewani T. , John-Aryankalayil Molykutty , Makinde Adeola Y. , Falduto Michael T. , Magnuson Scott R. , Coleman C. Norman
Published: April 2023

PDF - 29K, Differentially expressed Target mRNAs and regulatory miRNAs (showing inverse correlation) from cardiovascular pathways in HCAEC at 24h following SD and MF irradiation were identified by Target Filter Analysis program.
Supplementary Table 3 from Differential Expression of Stress and Immune Response Pathway Transcripts and miRNAs in Normal Human Endothelial Cells Subjected to Fractionated or Single-Dose Radiation
Authors: Palayoor Sanjeewani T. , John-Aryankalayil Molykutty , Makinde Adeola Y. , Falduto Michael T. , Magnuson Scott R. , Coleman C. Norman
Published: April 2023

PDF - 46K, Microarray data depicting gene expression fold changes respective to control group in genes from immune response category following SD and MF radiation exposure at 6h and 24h.
Supplemental Figure 1: from β-Arrestin-2 Counters CXCR7-Mediated EGFR Transactivation and Proliferation
Authors: Kallifatidis Georgios , Munoz Daniel , Singh Rajendra Kumar , Salazar Nicole , Hoy James J. , Lokeshwar Bal L.
Published: April 2023

Quantification of Western blot analysis from Figure 2.
Supplementary Figure 1 from PI3K and Bcl-2 Inhibition Primes Glioblastoma Cells to Apoptosis through Downregulation of Mcl-1 and Phospho-BAD
Authors: Pareja Fresia , Macleod David , Shu Chang , Crary John F. , Canoll Peter D. , Ross Alonzo H. , Siegelin Markus D.
Published: April 2023

PDF file - 1151K, S1. A) Protein lystates from three established glioblastoma cell lines (U87, LN229, U373), two glioblastoma neurosphere cultures (NCH644 and NCH690) as well as one ex-vivo low-passage glioblastoma neurosphere cell line (GS9-6) were harvested and subjected to immunoblotting for ph-Akt Serine 473, Akt, ph-BAD Serine 136, ph-BAD Serine 112 BAD and Mcl-1. B) U373 cells were treated with increasing concentrations of GDC-0941 (μM concentrations) for 7 hours and then subjected to immunoblotting with antibodies against ph-AKT (Serine 473), Akt and Mcl-1. 14-3-3β protein served as a loading control. C) U373 cells were treated with increasing concentrations of ABT-263 (μM concentrations) for 7 hours and subjected to immunoblotting with antibodies against phosphorylated Akt (Serine 473), Akt and Mcl-1. 14-3-3 protein served as loading control. D) LN229 cells were treated with increasing concentrations of ABT-263 (μM concentrations) for 7 hours and subjected to immunoblotting with antibodies against phosphorylated Akt (Serine 473), Akt and Mcl-1. 14-3-3 protein served as loading control. ABT - ABT-263; GDC - GDC-0941.
Figure S5 from MEK and TAK1 Regulate Apoptosis in Colon Cancer Cells with KRAS-Dependent Activation of Proinflammatory Signaling
Authors: McNew Kelsey L. , Whipple William J. , Mehta Anita K. , Grant Trevor J. , Ray Leah , Kenny Connor , Singh Anurag
Published: April 2023

Computational analysis of gene expression in primary colon tumor cohorts.
Supplemental Figure 6 from Tumor Suppressor Activity of Selenbp1, a Direct Nkx2-1 Target, in Lung Adenocarcinoma
Authors: Caswell Deborah R. , Chuang Chen-Hua , Ma Rosanna K. , Winters Ian P. , Snyder Eric L. , Winslow Monte M.
Published: April 2023

Nkx2-1 and its target Selenbp1 inhibit migration.
Supplementary Tables 1 - 2 from PTEN Is a Potent Suppressor of Small Cell Lung Cancer
Authors: Cui Min , Augert Arnaud , Rongione Michael , Conkrite Karina , Parazzoli Susan , Nikitin Alexander Yu. , Ingolia Nicholas , MacPherson David
Published: April 2023

XLSX file - 38KB, Supplemental Table 1. Primer sequences used for Real Time PCR Supplemental Table 2. Coverage of mouse exome samples.
Supplementary Figures 1 - 4 from PTEN Is a Potent Suppressor of Small Cell Lung Cancer
Authors: Cui Min , Augert Arnaud , Rongione Michael , Conkrite Karina , Parazzoli Susan , Nikitin Alexander Yu. , Ingolia Nicholas , MacPherson David
Published: April 2023

PDF file - 1172KB, S1. Neuroendocrine lung tumors in Rb/p53 AdCre model. S2. Neuroendocrine lung tumors in Rb/p53/Ptenlox/+AdCre model. S3. Analyses of Rb, p53 and Pten recombination in lung tumors. S4. Comparison of Rb/p53/Ptenlox/+ vs. Rb/p53/Ptenlox/lox lung tumors for expression of neuroendocrine and other lung cancer markers.
Figure S4 from EGFR Mutations Compromise Hypoxia-Associated Radiation Resistance through Impaired Replication Fork–Associated DNA Damage Repair
Authors: Saki Mohammad , Makino Haruhiko , Javvadi Prashanthi , Tomimatsu Nozomi , Ding Liang-Hao , Clark Jennifer E. , Gavin Elaine , Takeda Kenichi , Andrews Joel , Saha Debabrata , Story Michael D. , Burma Sandeep , Nirodi Chaitanya S.
Published: April 2023

MT-EGFR expression in HBEC cells is associated with dramatically elevated levels of replication factors.
Figure S3 from EGFR Mutations Compromise Hypoxia-Associated Radiation Resistance through Impaired Replication Fork–Associated DNA Damage Repair
Authors: Saki Mohammad , Makino Haruhiko , Javvadi Prashanthi , Tomimatsu Nozomi , Ding Liang-Hao , Clark Jennifer E. , Gavin Elaine , Takeda Kenichi , Andrews Joel , Saha Debabrata , Story Michael D. , Burma Sandeep , Nirodi Chaitanya S.
Published: April 2023

Camptothecin and hydroxyurea have differing effects on RPA associated H2AX foci in WT-EGFR expressing S-phase cells.
Figure S2 from EGFR Mutations Compromise Hypoxia-Associated Radiation Resistance through Impaired Replication Fork–Associated DNA Damage Repair
Authors: Saki Mohammad , Makino Haruhiko , Javvadi Prashanthi , Tomimatsu Nozomi , Ding Liang-Hao , Clark Jennifer E. , Gavin Elaine , Takeda Kenichi , Andrews Joel , Saha Debabrata , Story Michael D. , Burma Sandeep , Nirodi Chaitanya S.
Published: April 2023

MT-EGFR expression has marginal effects on endogenous reducing environment in NSCLCs or HBEC.
Supplemental Table 2 from Proteomic Characterization of Head and Neck Cancer Patient–Derived Xenografts
Authors: Li Hua , Wheeler Sarah , Park Yongseok , Ju Zhenlin , Thomas Sufi M. , Fichera Michele , Egloff Ann M. , Lui Vivian W. , Duvvuri Umamaheswar , Bauman Julie E. , Mills Gordon B. , Grandis Jennifer R.
Published: April 2023

Proteins expressed differentially in both primary HNSCC and PDXs.
Reconstitution of Flag-tagged wild-type hnRNP E1 in E1KD cells rescues cell cycle-dependent regulation of Cdc27 expression. from PCBP1/HNRNP E1 Protects Chromosomal Integrity by Translational Regulation of CDC27
Authors: Link Laura A. , Howley Breege V. , Hussey George S. , Howe Philip H.
Published: April 2023

S1. Immunoblot analysis of whole cell extracts isolated from G0-synchronized E1KD and KIWT (E1KD cells transfected with wild-type hnRNP E1) cells for Cdc27, Flag, and GAPDH.