bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigation of gaseous pollutants in residential-industrial area: Ambient levels, temporal variation and health risk assessment
Authors: Tarassoli Ahmad , Esmaili Sari Abbas , Bahramifar Nader
Published: July 2019

Introduction: The purpose of the current study was to investigate the ambient concentration levels of ground-level ozone (GLO), nitrogen dioxide, and sulfur dioxide with temporal variations and to determine the risk of exposure to their pollutant on public people living in this area. Materials and methods: In the present study, GLO, NOx and SO2 concentrations were monitored using an ambient analyzer during the period of April to September 2018. Results: The obtained results demonstrated that the average concentrations of SO2, NO2 and GLO in the INZ station was significantly greater than the two other evaluated stations (i.e. SHV and CMC). The corresponding values for the measured parameters in INZ station was almost 8.9, 7.2 and 11.9 times higher than those in SHV station, however, 4.8, 5.3 and 2.9 times greater than of ones in CMC station, respectively. The average values of SO2 and NO2 concentrations in the INZ varied from 97.2 to 128.1 μg/m3 in the evening hours and from 50.2 to 62.3 μg/m3 in the morning hours respectively. Also, the lowest concentration of NO2 was observed during afternoon hours when GLO showed a peak. The maximum pikes of GLO concentration were observed at 13:00 PM with 249.3 μg/m3. Results of human health risk assessment indicated acceptable risk (hazard quotient (HQ) values˂ 1) for non-carcinogenic adverse health effect. Conclusion: The findings in the present study can be useful in developing control-based strategies for primary pollutant emissions, and also GLO formation, improve air quality and reducing possible risks on human health. Policymakers should enforce the limits on the release of pollutants into the atmosphere in the study area by strengthening existing legislation.
The Influence of Light on Apoptosis in Preimplantation Mouse Embryos In Vitro
Authors: Khalili-Savadkouhi Sepideh , Karimpour Malekshah Abbasali , Mirhoseini Mehri , Moosazadeh Mahmood , Shahidi Maryam
Published: July 2019

Background: In vitro culture of mammalian embryos can slow or stop growth completely. This may be due to the medium used, pH, temperature, or light. There is considerable concern about the harmful effect of light in the laboratory environment. Cell number and apoptosis are useful parameters that indicate embryonic development and health. In this study, we assessed these two factors in the blastocyst. Materials and methods: A total of 128 embryos were extracted from NMRI mice at the 2-cell stage and were divided into 4 groups. The embryos were exposed to light for 0, 5, 15, and 30 min, and then cultured for 96 h. The degree of embryonic development were recorded every 24 h. Furthermore, several morphologically normal blastocysts were evaluated using the TUNEL assay. Results: There was no significant difference in developmental stages between the experimental and control groups. An evaluation of the percentage of blastomeres and apoptotic cells revealed significant differences among the four groups. The maximum number of apoptotic blastomeres was observed in the group exposed to light for 30 minutes. Conclusion: Up to thirty minutes of white fluorescent light can induce apoptosis in blastomeres, but it does not prevent embryo development.
L-Asparaginase-producing Rouxiella Species Isolation, Antileukemia Activity Evaluation, and Enzyme Production Optimization
Authors: Gilavand Farhad , Marzban Abdolrazagh , Kavyanifard Amirarsalan
Published: July 2019

Background: L-Asparaginase (L-Asp) is used as an efficient anti-cancer drug, especially for acute lymphoblastic leukemia (ALL). Currently, two bacterial asparaginase isoenzymes are used for cancer treatment. Therefore, this research focused on isolating native bacteria with the ability to produce L-Asp. Materials and Methods: L-Asp producing bacteria were isolated from soil samples on 9K medium supplemented with L-Asp as nitrogen source. Detection of L-Asp activity was performed by observing color change of the agar medium from yellow to orange due to the release of ammonia around the colonies. After the isolation and identification of the bacterium, L-Asp production was first optimized by the one factor-at-the-time (OFAT) technique followed by the response surface method. Next, the enzyme was extracted, purified, and assessed for antileukemia activity on U937 and MRC-5 cell lines. Results: The results revealed that L-Asp produced by Rouxiella sp. AF1 significantly inhibited the growth of U937 cells at a dose of up to 0.04 IU/ml, while MRC-5 was not affected at any enzyme doses. The final purification of the enzyme was achieved by column chromatography (Sephadex G-100) at approximately 0.31 mg/ml, and its specific activity was determined to be 0.51 IU/mg. The OFAT optimization experiments were performed primarily to determine optimal enzyme conditions, which were found to be neutral pH (pH7), 30 ∘C temperature, and 3 % NaCl, 1 % peptone, and 1% glucose concentrations. Statistical optimization was based on five factors obtained from OFAT, and response surface method (RSM) analysis introduced a quadratic model for enzyme production at the optimal range of these variables. This model provided an equation for measuring the effect of physiochemical conditions on final enzyme production. Conclusion: We showed that native bacteria may be novel candidates for isolating new metabolites such as L-Asp. Because many bacteria grow in unknown environments with unique ecological properties, the probability of discovering novel bacterial species producing bioactive compounds is high.
Down-regulation of miR-193b-3p and miR-376a-3p in Chronic Lymphocytic Leukemia
Authors: Namazi Faezeh , Hadi Nasrin , Moghimi Mansour , Eshaghiyan Amir , Nateghi Behnaz
Published: July 2019

Background: Chronic lymphocytic leukemia (CLL) is the most common adult human leukemia. Studies revealed that microRNAs (miRNAs) can function as oncogenes or tumor suppressors in CLL and that the expression of miRNAs, such as miR-193b-3p and miR-376a-3p change in several diseases. We aimed to elucidate the changes in miR- 193b-3p and miR-376a-3p expression in CLL and determine their potential as diagnostic biomarkers for this disease. Materials and Methods: We investigated miR-193b-3p and miR-376a-3p expression by quantitative real-time PCR in peripheral blood mononuclear cells of 30 patients with CLL and 30 healthy individuals. Moreover, in silico molecular enrichment analysis was conducted on predicted and validated targets of miR-193b-3p and miR-376a-3p from the miRecords and miRTarBase databases. Results: The expression of miR-193b-3p and miR-376a-3p was significantly different between the two groups (P<0.0001 and P < 0.0001, respectively). Conclusion: Based on these findings, miR-193b-3p and miR-376a-3p could be novel biomarkers for the early diagnosis of CLL and could be used to design new CLL control strategies.
Anticancer Effects of Moderate Static Magnetic Field on Cancer Cells In Vitro
Authors: Zafari Jaber , Vazini Hossein , Javani Jouni Fatemeh , Abdolmaleki Parviz , Monajemi Ramesh , Shams Elahe , Satari Mohammad
Published: July 2019

Background: Expansion of the use of magnetic fields in metals, mining, transport, research, and medicine industries has led to a discussion about the effects of magnetic fields and whether their strength is negligible. The aim of this study was to investigate the effects of magnetic field on the viability and proliferation rate of HeLa cells. Materials and Methods: We studied the effects of magnetic field on the viability, proliferation rate and membrane lipid peroxidation of cells, thus, HeLa cells (cancer cells) and human fibroblast cells (normal cells) were used. Initially, the cells were cultured in DMEM and to determine the impact of the magnetic field, the cells were treated with magnetic field at 4 specific intensity levels (0, 7, 14 and 21 mT) and 2 exposure times (24 h and 48 h). The viability percentage and inhibition of cell proliferation were calculated by MTT assay and Trypan blue staining, respectively. Results: Lipid peroxidation of the cell membrane was examined by malondialdehyde (MDA) method. As the intensity and exposure time of the static magnetic field (SMF) increased, the viability percentage and proliferation rate decreased and the lipid peroxidation levels increased in the Hela cells. Conclusion: In this study, we have shown the anticancer effects of static magnetic field and propose a suitable intensity range that can be effective for the treatment of cancer.
Use of Protein-Protein Interaction Network for Biomarker Identification in Oocyte Maturation
Authors: Farhadi Golzar , Fayazi Jamal , Allah Roshanfekr Hedayat , Nazari Mahmoud , Behdani Elham
Published: July 2019

Background: Oocyte maturation begins at the embryonic stage and continues throughout life. The effect of Follicle- Stimulating hormone (FSH) on gene of genes was evaluated using GEO access codes for the data set GSE38345. Materials and Methods: The microarray data containing the gene expression information from cow oocytes show that their maturation is influenced by FSH. Data analysis was performed using GEO2R. After identifying the genes and examining the different genes expressed, two gene groups with increased and decreased expression were identified. The interaction of each of the gene groups was examined using the STRING database, based on the co-expression information. The meaningful sub networks were explored using the Clusterone software. Gene ontology was performed using the comparative GO database. The miRNA-mRNA interaction network was also studied based on the miRWalk database. Finally, meaningful networks and subnets obtained by the Cytoscape software, were designed. Results: Comparison of oocyte gene expression data between the pre-maturation and postmaturation stages after treatment with FSH revealed 5958 genes with increased expression and 4275 genes with decreased expression. Examination of the protein interaction network among the set of increased and decreased expression genes based on string information revealed 262 genes with increased expression and 147 genes with decreased expression (high confidence (0.7) data). RPS3, NUSAP1, TBL3, and ATP5H showed increased expression and were effective in the positive regulation of rRNA processing, cell division, mitochondrial ATP synthesis coupled proton, and in oxidative phosphorylation and progesterone-mediated functions. WDR46 and MRPL22 showed decreased expression, which were important in the regulation of SRP-dependent co-translational proteins targeting the membrane, RNA secondary structure, unwinding, and functional pathways of ribosomal and RNA polymerase. The most important miRNA genes in the protein network of increased and decreased gene expression were bta-miR-10b-5p and miR-29b-2-5p. Conclusion: Examination of the genes expressed in the oocyte maturation pathway revealed nuclear, mitochondrial, and miRNA genes. Increasing and decreasing gene expression helps maintain equilibrium, which can be a biological marker.
Sistema Integrado para o Controle de Fluxo de Potência entre Veículo Elétrico, Rede Elétrica Pública e Residência
Authors: Pelegrino Lucas S. S. , Heldwein Marcelo L. , Waltrich Gierri
Published: July 2019

Esse artigo propõe um sistema, externo ao Veículo Elétrico (VE), capaz de adicionar funcionalidades de Vehicle-to-Home (V2H) e Vehicle-to-Grid (V2G) ao VE através do uso dos terminais da bateria disponíveis em um conector combo comercial. No caso do sistema proposto, esses terminais seriam usados para retirar energia dos veículos e entregá-la à rede ou manter a tensão na residência em caso de falta de energia. Esta configuração elimina a necessidade de conversores bidirecionais internos ao VE, reduzindo peso, custo e complexidade, adicionando também a possibilidade de integrar geração de energias renováveis locais. Para validar o conceito do sistema, são desenvolvidos o projeto e a simulação do sistema completo, assim como a construção dos conversores CC-CC utilizados no teste de um sistema de 2 kW, integrando os vários conversores de potência necessários.
Preoperative and postoperative features of non-functioning pituitary adenomas: a single center experience
Authors: ŞİŞMAN Pınar , ÖZBİÇER Buket , ÖZ GÜL Özen , CANDER Soner , SOYAK Halime , ERSOY Canan
Published: July 2019

Objectives:
The main purposes of surgical treatment for non-functioning
pituitary adenomas are removal of the pressure on the surrounding structures,
especially the hypophyseal gland and visual tissue, and the normalization of
hypophyseal functions. In our study, we retrospectively reviewed postsurgical
follow-up of patients with non-functioning pituitary adenoma treated at our
institution in a period of 15 years of monitoring and evaluated surgical
success, complication rate and recurrence rates in accordance with the
literature. Methods: This study
included 55 patients who had undergone surgery between 2000 and 2014 and who
were followed-up postoperatively at our center. Preoperative and postoperative
anterior pituitary hormones, complete resection and recurrence and also
postoperative recovery of endocrinological and ophthalmological functions were
statistically evaluated using file data of the patients. Results: There were 33 (60%) males and 22 (40%)
females. It was observed that the adenoma caused pressure
on the optic chiasm in 11 patients and infiltrated cavernous sinus in 9 patients.
Postoperative mean follow-up was 75.14 ± 43.01 months. Seventeen (30.9%) patients
had recurrence after complete resection. Postoperative persistence and
deterioration rates were 12.2% and 26.6% in adrenal insufficiency, 12.2% and
26.6% in central hypothyroidism, respectively, while 12.2% worsening in central
hypogonadism. Ophthalmologic findings were resolved in 62.5% of patients and
persisted in 37.5% of the patients.

Conclusions: The
adenoma size and experience of the surgeon in non-functioning pituitary
adenomas are the most important factors affecting surgical success. We
recommend that operations should be performed in experienced centers,
preoperative and postoperative endocrinological evaluations and long-term
follow-up should be done.
Human Capital Spillovers and Human Development Index in Yogyakarta Special Region and Central Java
Authors: Susanto Joko , Welly Udjianto Didit
Published: July 2019

This study analyzes the spillover of human capital in the Yogyakarta Special Region and Central Java. The research data includes the Human Development Index (HDI), capital, the ratio of college graduate workers, and the number of medical personnel in 2017 published by the Central Bureau of Statistics (BPS). This study uses a Spatial Regression analysis that includes elements of human capital spillover estimated by Geoda software. The results showed that there was a spillover of human capital between regencies/cities in Yogyakarta Special Region and Central Java. Lambda coefficient value, in Spatial Error Model (SEM), amounting to 0.5074 indicates that the Human Development Index (HDI) score of each regency/city will get an influence of 0.5074 multiplied by the HDI value of the neighboring regency/city. The capital variable is significant and exhibits that a rise in the HDI score will follow an increase in the number of capital. However, the ratio of college-educated worker and medical personnel is not significant. Due to the linkages between these provinces, the regency government and the provincial government should synergize each other, especially in the formulation of economic policies.
CÁLCULO DE PÉRDIDAS INDIRECTAS OCASIONADAS POR MOVIMIENTOS SÍSMICOS
Authors: García Pérez Jaime , García López Eric
Published: July 2019

En el criterio de la minimización del costo total para la obtención de coeficientes de diseño sísmico, se consideran tanto costos iniciales como costos de las pérdidas ocasionadas por los temblores. En estos últimos se encuentran las pérdidas indirectas denominadas intangibles que incluyen vidas humanas. Este trabajo presenta métodos para calcular el valor que la sociedad está dispuesta a invertir para salvar estas vidas. Se analizan tanto los problemas individuales como sociales, poniendo énfasis en los primeros, donde el valor de la vida se puede inferir a partir de lo que la persona está dispuesta a aceptar o a pagar para reducir un riesgo a morir. Puesto que estos métodos emplean curvas de utilidad, se pone atención a ellas discutiendo los requisitos que deben cumplir. Asimismo, se discuten los casos del impacto personal, legados y herencias cuando se emplean las curvas de utilidad. Finalmente se presentan ejemplos de aplicación.
NUEVAS EXPRESIONES DE LAS NTCM (2017) PARA EL CÁLCULO DE LA RESISTENCIA DE MUROS DIAFRAGMA DE MAMPOSTERÍA
Authors: Leal Graciano Jesus Martin , Pérez-Gavilán Escalante Juan José , Castorena González José Humberto , Reyes Salazar Alfredo , Barraza Guerrero Manuel A.
Published: July 2019

Se presentan las nuevas expresiones para calcular la resistencia a corte de muros diafragma de mampostería incluida en el Capítulo 4 de las Normas Técnicas Complementarias para el Diseño y Construcción de Estructuras de Mampostería 2017 del Gobierno de la Ciudad de México. Se consideran tres modos de falla: por aplastamiento, por deslizamiento y por tensión diagonal. Las expresiones se adaptaron de las especificadas en el código canadiense, utilizando los parámetros de resistencia usados en la norma mexicana, y se refiere a una resistencia a corte en los tres modos de falla. Se verificó que los resultados de resistencia calculados con estas expresiones dan resultados satisfactorios, al compararlos con resultados de una campaña experimental que también se presentan aquí. La campaña experimental incluyó seis especímenes, escala 1:2, en los que las variables de estudio fueron la rigidez relativa muro/marco, el uso de elementos de confinamiento y el refuerzo horizontal. Los resultados indican que la rigidez relativa muro/marco tiene un efecto relevante en la resistencia al agrietamiento del muro y la resistencia máxima del sistema. Adicionalmente, la contribución del refuerzo horizontal a la resistencia lateral depende de la rigidez relativa muro/marco, un fenómeno que las expresiones de diseño no toman en cuenta. Los elementos de confinamiento no incrementan la resistencia lateral ni la capacidad de desplazamiento del sistema; sin embargo, las dalas y castillos mejoran la estabilidad fuera del plano del muro y el contacto entre el muro y el marco.
The Prevalence of Smoking in 41-43-Year-Old Males and its Relation to Some Risk Factors
Authors: Alexandrov A. A. , Kotova M. V. , Ivanova E. I. , Rozanov V. B.
Published: July 2019

Aim. To evaluate the prevalence of smoking in 41-43-year-old men living in Moscow and its association with other chronic non-communicable diseases risk factors.Material and methods. People of male sex were prospectively followed up during 32 years since childhood (11-12 years). Only 301 (30%) representatives of 1005 initially enrolled people were examined after 32 years. The examination included: survey by a standard questionnaire (the passport data, the level of physical activity, bad habits – smoking, alcohol consumption); three-time blood pressure measurement; assessment of heart rate; measurement of weight and height, thickness of skin folds (over the triceps, under the scapula and on the abdomen); waist circumference.Results. Almost 50% of 41-44-year-old males smoked every day; less than a third of the study participants had never smoked. Every fifth of the smokers smoked less than 10 cigarettes per day while more than a half of them – a pack and more. The vast majority of the past smokers (85.2%) had stopped smoking at their own will and only very few (1.2%) by doctor’s advice. The intensity of smoking according to the pack/year index was >10 in almost 81.9% of the current smokers. The past smokers had abdominal obesity significantly more often than the non-smokers and the current smokers (57.5% vs 37% and 50.7%, respectively). The current smokers revealed the significantly higher risk of hypertension. The past smokers had 2.5 times higher risk of general obesity and 2.3 times higher one – of abdominal obesity as compared to the non-smokers. The intensity of smoking at the present time correlated with heart rate (HR). The past smokers (who smoked ≥20 cigarettes/day) as compared to the non-smokers had significantly higher HR, the Quetelet index, thickness of skin fold over the triceps and on the abdomen. The currents smokers revealed correlation of the status of smoking with HR, while the past smokers – with such parameters as the Quetelet index, waist circumference, thickness of skin fold under the scapula and over the triceps and no correlation with HR. The current smokers had significantly higher the 10-year risk of death as compared to thepast smokers and the non-smokers. Conclusion. Smoking is one of the most important risk factors of development of chronic non-communicable diseases. The struggle against smoking must be promoted, while close attention should be paid to people who had stopped smoking as a group of risk of cardiovascular disease onset.
Effect of the Single-Pill Amlodipine/Valsartan Combination on Hypertrophy and Myocardial Deformation Characteristics in Middle-Aged Patients with Essential Arterial Hypertension
Authors: Kochetkov A. I. , Borisova E. V. , Ostroumova O. D. , Lopukhina M. V. , Piksina G. V.
Published: July 2019

Aim. To investigate the impact of amlodipine/valsartan single-pill combination (A/V SPC) on left ventricular hypertrophy (LVH) and left ventricular (LV) myocardial strain and stiffness parameters in naїve middle-aged patients with stage II grade 1-2 essential arterial hypertension (EAH).Material and methods. A group of patients with stage II grade 1-2 EAH who had not previously received regular antihypertensive treatment (AHT) [n=38; mean age 49.7±7.0 years] was retrospectively formed. All the patients were treated with A/V SPC and all of them achieved target office blood pressure (BP) (less than 140/90 mm Hg). And after 12 weeks follow-up (since the time of reaching the target BP) the AHT effectiveness assessment, its impact on LVH and LV myocardial strain and stiffness parameters (general clinical data, ambulatory blood pressure monitoring, conventional and 2D-speckle tracking echocardiography) were performed in all included patients.Results. The number of patients with LVH significantly (p=0.039) decreased from 25 individuals (65.8%) at baseline to 15 patients (39.5%) at the end of follow-up. Among patients with LVH at baseline after the treatment with A/V SPC significantly decreased (p<0.001 for all) interventricular septum thickness (from 1.36±0.19 to 1.28±0.18 cm), LV posterior wall thickness (from 1.08±0.09 to 0.97±0.11 cm) and the LV myocardial mass index (from 123.3±19.3 to 110.8±20.8 g/m2). At the end of follow-up end-systolic elastance significantly (p<0.001) decreased from 4.01±1.12 to 3.46±0.88 mm Hg/ml. In the subgroup of patients with reduced (in absolute value) LV longitudinal 2D-strain (n=27) at baseline, there was a significantly (p=0.005) increasing in this parameter at the end of the study (from -16.14±2.21% to -17.30±2.13%, Δ%=8.45±13.35).Conclusion. In naive patients 40-65 years old with stage II grade 1-2 EAH AHT with A/V SPC provides effective 24 hours BP control, significantly reduced LVH and improves LV strain parameters, which indicates decreasing of LV myocardial stiffness.
Features of Thromboembolic and Hemorrhagic Complications in Planned Surgical Interventions in Patients with Atrial Fibrillation
Authors: Uddin L. N. , Egorov A. V. , Sokolova A. A. , Morozova N. S. , Napalkov D. A. , Fomin V. V.
Published: July 2019

Aim. To study the frequency and characteristics of the development of thromboembolic and hemorrhagic complications in patients with atrial fibrillation (AF) during planned surgical interventions.Material and methods. Preoperative examination and postoperative observation of 214 patients with AF (139 men and 75 women) who received anticoagulant therapy (ACT) and underwent planned surgery was carried out. The consistency of the used perioperative ACT regimens when performing interventions with current international recommendations was analyzed.Results. Complications developed in 33 patients (15.4%) from the total sample of 214 patients: in 14 (6.5%) thromboembolism, in 19 (8.9%) hemorrhagic complications. Analysis of the complication frequency in various types of surgical interventions showed that the frequency of thromboembolism during heart and vascular operations was 9.8% (4 cases), with interventions for malignant tumors – 7 (6.2%), while performing general surgical abdominal tumors operations – 3 (5.0%). The frequency of bleeding was maximum during operations for cancer 18.6%, this value was significantly higher compared with the frequency of these complications when performing general surgical abdominal operations – 3 cases (5.0%), and during interventions on the heart and vascular vessels bleedings were not observed. The total frequency of complications was also maximal when performing interventions for malignant tumors – 24.8%. This value was significantly higher than in the groups of patients who underwent general surgical abdominal operations – 10.0%, and interventions on the heart and vessels – 9.8%. Analysis of the frequency of compliance with the recommendations of the European Society of Cardiology in terms of ACT usage in patients with AF in the perioperative period and the structure of errors in choosing the ACT regimen showed that among the examined patients the recommendations were followed only in 102 (47.7%), while the frequency of non-recommended regimens use reached 52.3% (112 patients). The most frequently incorrectly used scheme was “bridge therapy” with the possibility of canceling oral anticoagulants in 107 (50.0%) patients.Conclusion. A significantly higher frequency of complications was found in patients, whose management did not follow the recommendations for perioperative ACT.
Effect of Hyperglycemia on the Total Surface Charge of the Erythrocyte Membrane in Patients with Metabolic Syndrome
Authors: Podzolkov V. I. , Koroleva T. V. , Kudryavtseva M. G.
Published: July 2019

Aim. To study the effect of hyperglycemia on the total surface charge of the erythrocyte membrane (SCEM) in patients with metabolic syndrome (MS).Material and methods. 112 MS patients were examined (45 men and 67 women) (mean age 61.4±7.2 years, average MS duration 8.7±5.2 years). The level of SCEM was determined by adsorption of a positive cationic dye (cationic blue O) on the surface of the plasma membrane of erythrocytes to completely neutralize their negative charge, followed by photometry of the solution and calculation of the number of charges on the cell surface of erythrocytes.Results. In the main group of patients with MS, abdominal obesity was observed in 100% of patients, arterial hypertension – in 73%, hyperglycemia – in 75%, dyslipidemia – in 80%. The level of glycated hemoglobin (HbA1c) was determined in all patients with MS, which was 7.3±1.9%. Patients with MS were conditionally divided according to the level of HbA1c into 2 groups (group 1 – HbA1c from 6.6 to 7.8%, group 2 – more than 7.8%). In MS patients with hyperglycemia, the SCEM values were significantly lower than in the group of patients without hyperglycemia (1.58±0.05×107 and 1.64±0.03×107, respectively; p=0.001)., Significant negative correlations between SCEM and the fasting blood glucose level, hyperglycemia duration, HbA1c level were found in patients with MS.Conclusion. SCEM indices reliably depended on the presence, severity and duration of hyperglycemia, which indicated the effect of impaired carbohydrate metabolism on the state of electric charge of erythrocyte membranes and, therefore, on the mechanisms of microvascular blood flow, thereby contributing to the development of vascular changes in patients with MS.
Vascular Remodeling Markers in Patients with Essential Arterial Hypertension Depending on Presence of Type 2 Diabetes Mellitus
Authors: Danilogorskaya Yu. A. , Zheleznykh E. A. , Privalova E. A. , Belenkov Yu. N. , Shchendrigina A. A. , Kozhevnikova M. V. , Shakaryants G. A. , Zektser V. Y. , Lishuta A. S. , Ilgisonis I. S.
Published: July 2019

Aim. To study some vascular remodeling markers in hypertensive patients depending on the presence of type 2 diabetes mellitus.Material and methods. The study included patients with essential hypertension 1-2 degrees of increase in blood pressure with/without type 2 diabetes (30 and 32 patients respectively). Using photoplethysmography in combination with reactive hyperemia test structural and functional markers of vascular remodeling were determined: in large vessels – stiffness index and phase shift; in microvessels – reflection index and the occlusion index. Using the computer video capillaroscopy of the nail bed the density of the capillary network at rest, after venous occlusion and after the test with reactive hyperemia was determined. ELISA was used for determining the level of humoral markers of endothelium dysfunction and vascular bed remodeling: metalloproteinase 9, metalloproteinase 9 inhibitor, E-selectin, endothelin, transforming growth factor (TGF-β1), endothelial growthfactor A.Results. In compare with hypertensive patients in hypertensive patients with type 2 diabetes significantly higher stiffness index values (11.15 [10.05; 12.35] vs 10.15 [8.83; 11.83] m/s; p=0.04) were found as well as significantly lower (p=0.00) capillary network density at rest (26.4 [24.2; 27.6] vs 35.1 [33.0; 45.0] cap/mm2; p=0.00) after the tests with reactive hyperemia (29 [24; 33.3] vs 40.0 [35.0;43.3] cap/mm2; p=0.00) and venous occlusion (32.5 [27.8; 34.5] vs 40.0 [33.0; 45.0] cap/mm2). In hypertensive patients with type 2 diabetes significantly higher levels of TFG-β1 (11648 [4117.8; 37933.8] vs 3938.5 [1808.8; 7694] pg/ml; p=0.00) and significantly lower levels of endothelin-1 (0,46 [0,29;1,3] vs 1.73 [0.63; 2.30] ng/ml; p=0.01) was detected in compare with hypertensive patients without type 2 diabetes.Conclusion. In both groups some signs of vascular remodeling were found at the level of both large arteries and microvessels (arterioles and capillaries). However, the group of hypertension + type 2 diabetes mellitus had statistically significantly more pronounced changes in arterial stiffness, capillary network density, as well as humoral markers levels of fibrosis and endothelial dysfunction.
The First Results of the Evaluation of Long-Term Nicorandil Treatment Effect on the Probability of Cardiovascular Complications in Patients with Stable Coronary Artery Disease (Data of Observational NIKEA Study)
Authors: Martsevich S. Yu. , Lukina Yu. V. , Kutishenko N. P. , Voronina V. P. , Dmitrieva N. A. , Zagrebelnyy A. V. , Lerman O. V. , Gaisenok O. V. , Gomova T. A. , Ezhov A. V. , Kuimov A. D. , Libis R. A. , Matyushin G. V. , Mitroshina T. N. , Nechaeva G. I. , Reznik I. I. , Skibitsky V. V. , Chesnikova A. I. , Dobrynina N. V. , Yakushin S. S.
Published: July 2019

Aim. To assess the influence of addition of nicorandil on long-term cardiovascular complications in patients with chronic coronary artery disease (CAD) in the framework of a prospective observational study.Material and methods. Prospective observational multicenter NIKEA study included 590 patients with stable CAD. All patients were recommended to add nicorandil to their baseline therapy. After 21 months, 547 telephone contacts were made, the life status of 524 people was specified, 23 patients were lost to follow-up. The following complications were registered: death from any cause; non-fatal myocardial infarction (MI); non-fatal stroke; urgent myocardial revascularization; hospitalization due to deterioration of CAD, chronic heart failure (CHF) or atrial fibrillation (AF). In 479 patients, a telephone survey was performed to identify adherence (persistence) to nicorandil– i.e. to verify the continuing the treatment for the prescribed duration.Results. During the follow-up period, 15 deaths were recorded, the causes of which were as follows: 3 CAD (2 MI, 1 left ventricular aneurysm), 1 pulmonary embolism, 2 CHF; 1 stroke, 1 oncology, 1 bilateral pneumonia. In the remaining 6 cases, the causes of death were not known. Cardiovascular (CV) complications were MI (8 cases in 7 patients, 1 patient had 2 MI in the follow-up period, 3 patients from this group died), stroke in 6 patients (7 cases, 1 patient had 2 strokes, 1 patient died). Urgent percutaneous coronary intervention was performed in 3 patients (in 2 patients due to acute MI, in 1 patient due to unstable angina). During the observation period, 21 people were urgently hospitalized due to deterioration of clinical condition. According to the results of a medical survey, by the end of the follow-up period, 237 people were not adherent to prescribed nicorandil, and 242 patients took the recommended medication (adherent patients). The main cardiovascular complications and death from any cause (р<0.001) as well as the number of urgent hospitalizations (р=0.017) were significantly more common in non-adherent to nicorandil patients in comparison with adherent patients.

Conclusion. The overall rate of CAD complications in NIKEA study was typical for patients with stable CAD receiving contemporary medical therapy. Addition of nicorandil significantly reduced the rate of CV complications in adherent patients in comparison with non-adherent patients.
Systemic ATTR-amyloidosis, a Rare Form of Internal Organ Damage
Authors: Rameev V. V. , Myasnikov R. P. , Vinogradov P. P. , Kozlovskaya L. V. , Moiseev S. V. , Fomicheva E. I. , Beregovskaya S. A. , Mershina E. A. , Kostina S. A. , Strizhakov L. A. , Rameeva A. S. , Tao P. P. , Drapkina O. M.
Published: July 2019

The article presents the case report of a rare hereditary form of systemic ATTR-amyloidosis in Russian patient with a discussion of approaches to the diagnosis and treatment of this form, also based on the own experience in the management of such patients. Modern ideas about the pathogenesis of the disease as well as detailed information about the clinical manifestations of amyloid cardiopathy and of other organs are presented. The nature of structural and hemodynamic changes in the heart is discussed on the basis of experience, including own, ultrasound examination of the heart in patients with amyloidosis, especially the article focuses the reader's attention on the true infiltrative nature of transtiretin amyloid cardiopathy in contrast to AL-amyloidosis, in which there is a significant inflammatory component that determines a more unfavorable natural course of AL-amyloidosis of the heart. The article discusses the differential diagnosis of different types of amyloidosis, the diagnostic difficulties associated with weak congophilia of transtiretin amyloidosis and at the same time substantiates the need for morphological verification of the diagnosis. Modern methods of treatment of ATTR-amyloidosis are discussed.
Atherosclerosis and Osteoporosis. Common Targets for the Effects of Cardiovascular and Anti-Osteoporotic Drugs (Part II). The Effect of Antiosteoporotic Drugs on the Vascular Wall State
Authors: Skripnikova I. A. , Kosmatova O. V. , Kolchinа M. A. , Myagkova M. A. , Alikhanova N. A.
Published: July 2019

In the second part of the literature review, data are presented on the possible effect of anti-osteoporosis therapy on the vascular wall and the development of calcification. The discovery of common biological substances involved in the development of atherosclerosis, calcification of the vascular wall and osteoporosis attracts the attention of scientists in terms of targets for assessing the effects of already known drugs or developing new drugs that can simultaneously prevent or slow the progression of both atherosclerosis and osteoporosis. Currently, various groups of drugs for the treatment of osteoporosis have been studied to prevent or reduce the progression of subclinical atherosclerosis and calcification. Both antiresorptive drugs (bisphosphonates, monoclonal antibodies to RANKL, selective estrogen receptor modulators), and bone-anabolic therapy, which includes teriparatide, were studied. However, there are a few such studies and the most promising drugs that have a preventive effect in the early stages of atherosclerotic damage are bisphosphonates. Other classes of antiosteoporotic drugs did not reveal a positive effect on the vascular wall, and some of them increased the cardiovascular risk. Divergences in the results of experimental and clinical studies attract attention. If in the experiment almost all drugs for the treatment of osteoporosis had an atheroprotective effect and suppressed vascular calcification, then in clinical conditions only bisphosphonates confirmed the positive effect on the vascular wall.
Features of Atrial Fibrillation in Patients with Arterial Hypertension and Extracardial Disorders
Authors: Khidirova L. D. , Yakhontov D. A. , Zenin S. A. , Mamedov M. N.
Published: July 2019

Aim. To study the features of the progression of atrial fibrillation (AF) in patients with arterial hypertension (HT) and comorbid extracardiac diseases.Material and methods. In the observational cohort study 308 men 45-65 years old with AF and HT were observed Most patients also had the following comorbid extracardiac diseases: diabetes mellitus (DM; n=40), diffuse toxic goiter (DTG; n=42); hypothyroidism (HTH; n=59), abdominal obesity (AO; n=64), and chronic obstructive pulmonary disease (COPD; n=47). The comparison group consisted of 56 patients with AF and HT only. Clinical, anthropometric, laboratory parameters, levels of NT-proBNP and galectin-3, results of ECG, daily monitoring of ECG and blood pressure wereassessed initially and after 12 months.Results. Persistent AF (29.6-65.2%) and long-term persistent AF (16-31.3%) were more common in groups with extracardiac diseases compared with the AF+HT group (20.4% and 14.2%, respectively). Permanent form of AF was statistically significantly more common in the DM (11.1%), AO (14.8%) and HTH (6.2%) groups compared with the AF+HT group (0.6%). A comparative assessment of risk factors (smoking and alcohol) did not show significant differences in prevalence in the groups (22-44%). An assessment of the levels of fibrosis and remodeling markers found a statistically significant increase in the level of galectin-3 in groups of patients with concomitant extracardiac diseases and an increase in the NT-proBNP level, relative to reference values in all groups except DTG.Conclusion. Patients with AF in combination with HT and comorbid extracardiac diseases have a more rapid AF progression.
Disorders of the Cardiovascular System in Patients with Chronic Viral Hepatitis
Authors: Chistyakova M. V. , Govorin A. V. , Parkhomenko Yu. V.
Published: July 2019

Aim. To study some structural and functional parameters of the heart and the parameters of hepato-lienal blood flow in patients with chronic viral hepatitis (CVH).Material and methods. 74 patients with CVH were examined, the average age was 41 years, the disease duration – 3.6 years. The patients were divided into 2 groups: group 1 – patients with minimal biochemical activity of hepatitis (n=39); group 2 – patients with moderate hepatitis activity (n=35). The control group included 22 healthy patients. Parameters of tissue doppler echocardiography, hepato-lienal blood flow were evaluated depending on the laboratory activity of CVH.Results. The systolic velocity of the fibrous ring displacement of the tricuspid valve in patients of groups 1 and 2 was lower than in the control by 15% and 17%, respectively (p<0.001). Em decreased in groups 1 and 2 in comparison with healthy individuals in the basal segment of the left ventricle lateral wall, on the mitral valve fibrous ring, in the basal segment of the interventricular septum (p<0.001). The Em/Am ratio decreased in patients of groups 1 and 2 compared with healthy patients in the basal segment of the left ventricle lateral wall, in the medial segment of the interventricular septum, on fibrous ring of the tricuspid valve, in the basal and medial segments of the right ventricular lateral wall (p<0.001). Isovolumic contraction and relaxation time as well as the volume index of the left atrium were increased in patients of the 1st and 2nd groups in comparison with control; more severe violations were observed in patients of the 2nd group (p<0.001). In patients of both groups, acceleration of blood flow and dilation of arteries and veins in the hepato-lienal circulation system (p<0.001) were found. Besides, in patients with moderate hepatitis activity there was an acceleration of blood flow in the hepatic artery in comparison with the control (p<0.001).Conclusion. In patients with CVH the follow disorders were observed: decrease in the global longitudinal systolic function of the right ventricle, violation of diastolic relaxation of the ventricles, increase in the volume index of the left atrium, as well as expansion of the arteries and veins of the hepato-lienal circulation system. These violations were more pronounced in patients with a moderate laboratory activity of CVH. Correlations between cardiohemodynamic parameters, indicators of hepato-lienal blood flow and viral load were revealed.
Predicting Methods for Analyzing Data on Fatal Outcome Possibility in the Combination of Acute Coronary Syndrome and Atrial Fibrillation According to the Krasnodar Region Registry
Authors: Tatarintseva Z. G. , Kosmacheva E. D. , Kruchinova S. V. , Akinshina V. A. , Khalafyan A. A.
Published: July 2019

With the development of atrial fibrillation (AF), patients with acute coronary syndrome (ACS) are characterized by a twofold increase in the 30-day mortality compared with patients with sinus rhythm. In this regard, there is great interest in developing models of risk stratification to identify adverse outcomes in these patients with a view to more careful monitoring of patients in this group.Material and methods. For the construction of predictive models, a statistical method was used for the classification trees and, the procedure for neural networks implemented in the STATISTICA package. For the construction of prognostic models, a sample was used, consisting of 201 patients with and without fatal outcome; condition of each patient was described by 42 quantitative and qualitative clinical indices. Each patient belonged to one of 3 groups according to the type of AF: new-onset AF in ACS patient, paroxysmal AF, documented in an anamnesis before the episode of ACS and the constant or persistent form of AF.Results. To determine predictors of models predicting the possible fatal outcome of a patient, the Spearman correlation coefficient was used. Examination of the correlations for each of the 3 groups separately allowed to reveal clinical indicators for each group – predictors of predictive models with predominantly moderate correlations to the categorical variable “lethal outcome”. After analyzing the prognostic ability of the developed models, a software module was created in the Microsoft Visual C # 2015 programming environment to determine lethal outcome possibility in patients with ACS in the presence of AF using classification trees and neural networks.Conclusion. It is shown that for patients with ACS in the presence of AF, it is possible to construct mathematically based prognostic models that can reliably predict the lethal outcome possibility in patients based on actual values of clinical indices. In this case, clinical indicators can be both quantitative and qualitative (categorical), breaking patients into certain categories. Similar applications, unlike risk scales, are mathematically justified and can form the basis of systems for supporting decision-making.
Medication Adherence: does Patient Participation in Randomized Clinical Trials Affect on it?
Authors: Vasyukova N. O. , Lukina Yu. V. , Kutishenko N. P. , Martsevich S. Yu. , Zvonareva O. I.
Published: July 2019

The article focuses on the problem of low adherence to treatment among patients, in general, and provides data on the high adherence among patients in randomized controlled trials. Low adherence remains one of the most debated and difficult problems to solve nowadays. Poor medication adherence of the patient can significantly worsen the effectiveness of treatment and leads to increased health care costs. And although the factors that improve adherence are varied, and include the skills of the doctor, the patient’s personal characteristics, the external environment, an individual approach to each patient and etc., the absence of a “gold standard” for assessing adherence in clinical practice makes it difficult to predict and significantly improve it among patients. Nevertheless, the article discusses the existing doctor-patient interaction model, which strictly regulates the algorithms and technical means to achieve the best medication adherence. Randomized clinical trials (RCT) are the basis of evidence-based medicine, the results obtained in RCT form the basis of existing clinical guidelines. While participating in RCT patients can receive comprehensive information about the disease, the effectiveness of drug therapy, possible side effects of the therapy being conducted, the research objectives and prognosis from their attending physician; patients are trained to be disciplined and adhere to the recommendations of the doctor. The RCT, for a number of objective reasons, uses a variety of methods for assessing adherence and ways to improve it, which leads to higher patient adherence then among patients in general.
Genetic, Epigenetic and Transcription Factors in Atrial Fibrillation
Authors: Sapelnikov O. V. , Kulikov A. A. , Favorova O. O. , Matveeva N. A. , Cherkashin D. I. , Nikolaeva O. A. , Akchurin R. S.
Published: July 2019

Atrial fibrillation (AF) is one of the most common arrhythmia that occurs in patients with cardiovascular diseases. Congenital forms of AF are quite rare. Many studies have shown that genetic, epigenetic and transcription factors may play an important role in the development and the progression of AF. In our review, studies have been conducted on the identification of mutations in ionic and non-ionic channels, possibly associated with AF. These mutations were found only in isolated groups of patients with AF, and in general, monogenic forms of AF are a rare subtype of the disease. Genomic association studies have helped to identify potential links between single nucleotide polymorphisms and AF. The risk of AF in the general population is likely to be determined by the interaction between environmental factors and many alleles. In recent years, the emergence of a genome-wide associative studies has significantly expanded the understanding of the genetic basis for the inheritance of AF and has led to the emergence of new evidence of the important role of genetic factors in the development of AF, in the risk stratification of AF and the recurrence of AF. Epigenetic factors are also important in AF. Epigenetic therapy aimed at treating a disease through exposure to epigenome is currently under development. A newly emerged area of ablatogenomics includes the use of genetic profiles that allow assessing the likelihood of recurrence of AF after catheter ablation. The results of genetic studies in AF show that, in addition to their role in the appearance of congenital heart pathologies, transcription factors play an important role in the pathogenesis of AF.