bluuebunny/crossref_metadata_2025 huggingface dataset:
De novo spatiotemporal modelling of cell-type signatures in the developmental human heart using graph convolutional neural networks
Authors: Marco Salas Sergio , Yuan Xiao , Sylven Christer , Nilsson Mats , Wählby Carolina , Partel Gabriele
Published: August 2022

With the emergence of high throughput single cell techniques, the understanding of the molecular and cellular diversity of mammalian organs have rapidly increased. In order to understand the spatial organization of this diversity, single cell data is often integrated with spatial data to create probabilistic cell maps. However, targeted cell typing approaches relying on existing single cell data achieve incomplete and biased maps that could mask the true diversity present in a tissue slide. Here we applied a de novo technique to spatially resolve and characterize cellular diversity of in situ sequencing data during human heart development. We obtained and made accessible well defined spatial cell-type maps of fetal hearts from 4.5 to 9 post conception weeks, not biased by probabilistic cell typing approaches. With our analysis, we could characterize previously unreported molecular diversity within cardiomyocytes and epicardial cells and identified their characteristic expression signatures, comparing them with specific subpopulations found in single cell RNA sequencing datasets. We further characterized the differentiation trajectories of epicardial cells, identifying a clear spatial component on it. All in all, our study provides a novel technique for conducting de novo spatial-temporal analyses in developmental tissue samples and a useful resource for online exploration of cell-type differentiation during heart development at sub-cellular image resolution.
A transient apical extracellular matrix relays cytoskeletal patterns to shape permanent acellular ridges on the surface of adult C. elegans
Authors: Katz Sophie S. , Barker Trevor J. , Maul-Newby Hannah M. , Sparacio Alessandro P. , Nguyen Ken C. Q. , Maybrun Chloe L. , Belfi Alexandra , Cohen Jennifer D. , Hall David H. , Sundaram Meera V. , Frand Alison R.
Published: August 2022

Epithelial cells secrete apical extracellular matrices to form protruding structures such as denticles, ridges, scales, or teeth. The mechanisms that shape these structures remain poorly understood. Here, we show how the actin cytoskeleton and a provisional matrix work together to sculpt acellular longitudinal alae ridges in the cuticle of adult C. elegans. Transient assembly of longitudinal actomyosin filaments in the underlying lateral epidermis accompanies deposition of the provisional matrix at the earliest stages of alae formation. Actin is required to pattern the provisional matrix into longitudinal bands that are initially offset from the pattern of longitudinal actin filaments. These bands appear ultrastructurally as alternating regions of adhesion and separation within laminated provisional matrix layers. The provisional matrix is required to establish these demarcated zones of adhesion and separation, which ultimately give rise to alae ridges and their intervening valleys, respectively. Provisional matrix proteins shape the alae ridges and valleys but are not present within the final structure. We propose a morphogenetic mechanism wherein cortical actin patterns are relayed to the laminated provisional matrix to set up distinct zones of matrix layer separation and accretion that shape a permanent and acellular matrix structure.
Intrageneric cross-reactivity of monospecific rabbit antisera against venoms of the medically most important Bitis spp. and Echis spp. African snakes
Authors: Gómez Aarón , Sánchez Andrés , Durán Gina , Cordero Daniel , Segura Álvaro , Vargas Mariángela , Solano Daniela , Herrera María , Chaves-Araya Stephanie , Villalta Mauren , Sánchez Melvin , Arguedas Mauricio , Díaz Cecilia , Gutiérrez José María , León Guillermo
Published: August 2022

Background

Snakebite envenomation exerts a heavy toll in sub-Saharan Africa. The design and production of effective polyspecific antivenoms for this region demand a better understanding of the immunological characteristics of the different venoms from the most medically important snakes, to select the most appropriate venom combinations for generating antivenoms of wide neutralizing scope. Bitis spp. and Echis spp. represent the most important viperid snake genera in Africa.

Methodology/Principal findings

Eight rabbit-derived monospecific antisera were raised against the venoms of four species of Bitis spp. and four species of Echis spp. The effects of immunization in the rabbits were assessed, as well as the development of antibody titers, as judged by immunochemical assays and neutralization of lethal, hemorrhagic, and in vitro coagulant effects. At the end of immunizations, local and pulmonary hemorrhage, together with slight increments in the plasma activity of creatine kinase (CK), were observed owing to the action of hemorrhagic and myotoxic venom components. Immunologic analyses revealed a considerable extent of cross-reactivity of monospecific antisera against heterologous venoms within each genus, although some antisera provided a more extensive cross-reactivity than others. The venoms that generated antisera with the broadest coverage were those of Bitis gabonica and B. rhinoceros within Bitis spp. and Echis leucogaster within Echis spp.

Conclusions/Significance

The methodology followed in this study provides a rational basis for the selection of the best combination of venoms for generating antivenoms of high cross-reactivity against viperid venoms in sub-Saharan Africa. Results suggest that the venoms of B. gabonica, B. rhinoceros, and E. leucogaster generate antisera with the broadest cross-reactivity within their genera. These experimental results in rabbits need to be translated to large animals used in antivenom production to assess whether these predictions are reproduced in horses or sheep.
Data augmentation using Variational Autoencoders for improvement of respiratory disease classification
Authors: Saldanha Jane , Chakraborty Shaunak , Patil Shruti , Kotecha Ketan , Kumar Satish , Nayyar Anand
Published: August 2022

Computerized auscultation of lung sounds is gaining importance today with the availability of lung sounds and its potential in overcoming the limitations of traditional diagnosis methods for respiratory diseases. The publicly available ICBHI respiratory sounds database is severely imbalanced, making it difficult for a deep learning model to generalize and provide reliable results. This work aims to synthesize respiratory sounds of various categories using variants of Variational Autoencoders like Multilayer Perceptron VAE (MLP-VAE), Convolutional VAE (CVAE) Conditional VAE and compare the influence of augmenting the imbalanced dataset on the performance of various lung sound classification models. We evaluated the quality of the synthetic respiratory sounds’ quality using metrics such as Fréchet Audio Distance (FAD), Cross-Correlation and Mel Cepstral Distortion. Our results showed that MLP-VAE achieved an average FAD of 12.42 over all classes, whereas Convolutional VAE and Conditional CVAE achieved an average FAD of 11.58 and 11.64 for all classes, respectively. A significant improvement in the classification performance metrics was observed upon augmenting the imbalanced dataset for certain minority classes and marginal improvement for the other classes. Hence, our work shows that deep learning-based lung sound classification models are not only a promising solution over traditional methods but can also achieve a significant performance boost upon augmenting an imbalanced training set.
Effects of sea salt intake on metabolites, steroid hormones, and gut microbiota in rats
Authors: Chanmuang Saoraya , Kim Bo-Min , Gu Su-Yeon , Son Ye-Jin , Le Huong-Giang , Nam Young-Do , Song Eun-Ji , Ham Kyung-Sik , Kim Hyun-Jin
Published: August 2022

High salt intake is positively linked to many health problems, but the effect of mineral-rich sea salt (SS) has rarely been studied. To better understand the physiological effects of SS intake, the changes in general characteristics, metabolites, steroid hormones, and gut microbiota of SS-fed rats were investigated. Male rats were fed either a normal diet (ND, control) or ND containing 1% SS or 4% SS for 5 weeks. SS intake decreased fat, spleen, liver, and body weight, and increased blood urea nitrogen (BUN), water intake, and gut salt content. Accumulated gut salt content led to a decrease in beneficial bacteria, such as Lachnospiraceae and Lactobacillus, but an increase in potentially harmful bacteria, resulting in a change in lipid metabolites associated with gut health. Interestingly, most renal lysophosphatidylcholines (LPCs) associated with many renal functions were dramatically decreased and female hormones, such as estrogens, were significantly more altered than the male hormones by high SS intake. Although further investigation is needed, these data suggest that high SS intake could be positively linked to kidney dysfunction and gut health problems, and salt-related physiological changes may be sex-specific. Additionally, these data will be useful to better under-stand the physiological effects of SS intake.
The prevalence of self-reported anxiety, depression, and associated factors among Hanoi Medical University’s students during the first wave of COVID-19 pandemic
Authors: Nguyen Dat Tien , Ngo Tri Minh , Nguyen Huong Lan Thi , Le Minh Dai , Duong Mai Le Ngoc , Hoang Phan Huy , Nguyen Ha Viet , Foster Kirsty , Duong Tuyen Van , Kim Giang Bao , Pham Tung Thanh
Published: August 2022

Background

Medical students are known to have higher levels of these issues than the general population but in Vietnam the effects of the pandemic on medical student mental health was not documented.

Objectives

To estimate the prevalence and identify factors associated with self-reported anxiety disorder, depression, and perception of worsening mental health among Vietnamese medical students during the COVID-19 pandemic.

Method

A cross-sectional study was conducted from April 7th to 29th, 2020. All students in Doctor of General Medicine, Doctor of Preventive Medicine, and Bachelor of Nursing tracks at Hanoi Medical University (3672 students) were invited to participate. Data were collected using an online questionnaire including demographic characteristics, Generalized Anxiety Disorder 7 items, Patient Health Questionnaire 9 items, Fear of COVID-19 scale, and question about worsening mental health status. Robust Poisson regression was used to assess the association between mental health status and associated factors.

Results

Among 1583 students (43.1% response rate), the prevalence of students screened positive for anxiety disorder was 7.3%(95%C.I.:6.0–8.7), depression was 14.5%(95%C.I.:12.8–16.3), and perceiving worsening mental health was 6.9%(95%C.I.:5.7–8.3). In multivariable regression models, significant factors associated with self-reported anxiety disorder included being male (PR = 1.99,95%C.I.:1.35–2.92), difficulty in paying for healthcare services (PR = 2.05,95%C.I.:1.39–3.01), and high level of fear of COVID-19 (Q3:PR = 2.36,95%C.I.:1.38–4.02 and Q4:PR = 4.75,95%C.I.:2.65–8.49). Significant factors associated with self-reported depression were difficulty in paying for healthcare services (PR = 1.78,95%C.I.:1.37–2.30), and high level of fear of COVID-19 (Q3:PR = 1.41,95%C.I.:1.02–1.95 and Q4:PR = 2.23,95%C.I.:1.51–3.29). Significant factors associated with perceived worsening mental health status included having clinical experience (PR = 1.83,95%C.I.:1.17–2.88) and having atypical symptoms of COVID-19 (PR = 1.96,95%C.I.:1.31–2.94).

Conclusion

The prevalence of self-reported depression, anxiety disorder, and worsening mental health among Vietnamese students during the first wave of COVID-19 was lower than in medical students in other countries. Further investigation is needed to confirm this finding.
Nicotinic receptor components of amyloid beta 42 proteome regulation in human neural cells
Authors: Sinclair Patricia , Kabbani Nadine
Published: August 2022

Alzheimer’s disease (AD) is associated with chronic neurodegeneration often accompanied by elevated levels of the neurotoxic peptide amyloid-beta 1–42 (Aβ42) in the brain. Studies show that extracellular Aβ42 binds to various cell surface receptors including the human α7 nicotinic acetylcholine receptor (nAChR) and activates pathways of neurotoxicity leading to cell death. The α7 nAChR is thus considered a promising drug target for therapy against neurodegenerative disease such as AD. In this study, we use mass spectrometry-based label-free precursor ion quantification to identify proteins and pathways that are changed by a 72-hour treatment with Aβ42 or Aβ42 in the presence of the α7 nAChR blocker, α-bungarotoxin (Bgtx) in the human neuroblastoma SH-SY5Y cell line. Bioinformatic gene ontology enrichment analysis was used to identify and characterize proteins and pathways altered by Aβ42 presentation. The results support evidence on the involvement of mitochondrial proteins in Aβ42 responses and define potential mechanisms of α7 nAChR mediated amyloid toxicity. These findings can inform pharmacological strategies for drug design and treatment against amyloid disease.
Applying novel connectivity networks to wood turtle populations to provide comprehensive conservation management strategies for species at risk
Authors: Bouchard Cindy , Lord Étienne , Tessier Nathalie , Lapointe François-Joseph
Published: August 2022

Genetic diversity within and among populations is frequently used in prioritization processes to rank populations based on their vulnerability or distinctiveness, however, connectivity and gene flow are rarely considered within these frameworks. Using a wood turtle (Glyptemys insculpta) population graph, we introduce BRIDES as a new tool to evaluate populations for conservation purpose without focusing solely on individual nodes. BRIDES characterizes different types of shortest paths among the nodes of a subgraph and compares the shortest paths among the same nodes in a complete network. The main objectives of this study were to (1) introduce a BRIDES selection process to assist conservation biologists in the prioritization of populations, and (2) use different centrality indices and node removal statistics to compare BRIDES results and assess gene flow among wood turtle populations. We constructed six population subgraphs and used a stepwise selection algorithm to choose the optimal number of additional nodes, representing different populations, required to maximize network connectivity under different weighting schemes. Our results demonstrate the robustness of the BRIDES selection process for a given scenario, while inconsistencies were observed among node-based metrics. Results showed repeated selection of certain wood turtle populations, which could have not been predicted following only genetic diversity and distinctiveness estimation, node-based metrics and node removal analysis. Contrary to centrality measures focusing on static networks, BRIDES allowed for the analysis of evolving networks. To our knowledge, this study is the first to apply graph theory for turtle conservation genetics. We show that population graphs can reveal complex gene flow dynamics and population resiliency to local extinction. As such, BRIDES offers an interesting complement to node-based metrics and node removal to better understand the global processes at play when addressing population prioritization frameworks.
Cross-sectional associations of physical frailty with fall, multiple falls and fall-injury among older Indian adults: Findings from LASI, 2018
Authors: Thakkar Shriya , T. Muhammad , Srivastava Shobhit
Published: August 2022

Background

Although there has been a range of studies that focused on physical frailty and associated fall outcomes within developed countries, similar studies from developing countries have been limited. This study aimed to examine the relationship between physical frailty and the prevalence of falls, multiple falls and fall-related injuries among the ageing population within the Indian context.

Methods

Individual-level data from the first wave of the Longitudinal Aging Study in India (LASI) with 28,285 older adults aged 60 years and above (male 48.9%) was used for this study. Physical frailty was assessed through the physical frailty phenotype adapted from Fried’s criteria. Multivariable logistic regression was employed to examine the association of frailty status with falls, multiple falls, and fall-related injuries among Indian older adults.

Results

The prevalence of frailty was found to be 29.94% within the sample and frail older adults had a higher prevalence of falls (15.43% vs 11.85%), multiple falls (7.73% vs 5.25%), and fall related injuries (6.68% vs 5.29%). The odds of falling among frail older adults were significantly higher in reference to the odds of falling among non-frail older adults [aOR: 1.24; CI: 1.09–1.41]. Similarly, the odds of multiple falls among frail older adults were significantly higher in reference to the odds of multiple falls among non-frail older adults [aOR: 1.24; CI: 1.05–1.48]. Moreover, the odds of fall-related injury among frail older adults were significantly higher in reference to the odds of fall-related injury among non-frail older adults [aOR: 1.21; CI: 1.01–1.45]. Falls, multiple falls and fall-related injuries were found to be significantly associated with employment and poor self-rated health, whereas, females and lone living older adults had a significantly higher likelihood of suffering from falls and multiple falls.

Conclusion

Older individuals with physical frailty were found to be at increased risk of falls, multiple falls and fall-related injuries in India. The findings of our study also have important clinical implications in the measures undertaken to reduce falls and enable future healthcare practitioners and policymakers to factor in the key determinant of physical frailty.
Anxiety and depression in Guatemala: Sociodemographic characteristics and service access
Authors: Naber Jonathan , Mactaggart Islay , Dionicio Carlos , Polack Sarah
Published: August 2022

Epidemiological data on depression and anxiety in Guatemala is lacking. Using 2016 National Disability Survey data, we explored the sociodemographics of people with anxiety and/or depression and its heightened burden on access to key services. The survey (n = 13,073) used the Washington Group Extended Set to estimate disability prevalence, including anxiety and/or depression. A nested case-control study was included to explore the impact of disability on key life areas. Cases (indicating ‘A lot of difficulty’ or ‘Cannot do’ in one or more functional domain) and age-/sex-matched controls were administered a structured questionnaire. Multivariable logistic regression and heightened-burden analysis were conducted. Higher odds of anxiety and/or depression were found in participants who were 50+ (aOR 2.3, 1.8–3.1), female (aOR 1.8, 1.4–2.2), urban (aOR 1.5, 1.2–1.9), divorced/separated (aOR 2.0, 1.3–3.0), and widowed (aOR 1.6, 1.0–2.4), as well as those with impaired communication or cognition (aOR 17.6, 13.0–23.8), self-care (aOR 13.2, 8.5–20.5), walking (aOR 13.3, 9.7–18.3), hearing (aOR 8.5, 5.6–13.1), and vision (aOR 8.5, 6.1–11.8). Lower odds of anxiety and/or depression were found in participants with a university education (aOR 0.2, 0.5–0.9), and those living in the southeast (aOR 0.2, 0.1–0.3) or northeast (aOR 0.3, 0.2–0.4). Compared to people with impairments that were not depression and/or anxiety, people with depression and/or anxiety were less likely to receive a retirement pension (aOR 0.4, 0.2–0.8), and more likely to receive medication for depression/anxiety (aOR 4.1, 1.9–9.1), report a serious health problem (aOR 1.8, 1.3–2.5), and seek advice/treatment with a government health worker/health post (aOR 6.3, 1.0–39.2).
Seroprevalence and longevity of SARS-CoV-2 nucleocapsid antigen-IgG among health care workers in a large COVID-19 public hospital in Saudi Arabia: A prospective cohort study
Authors: Alasmari Faisal , Mukahal Mahmoud , Alqurashi Alaa Ashraf , Huq Molla , Alabdrabalnabi Fatima , AlJurayyan Abdullah , Alkahtani Shymaa Moshobab , Assari Fatimah Salem , Bashaweeh Rahaf , Salam Rana , Aldera Solaf , Alkinani Ohud Mohammed , Almutairi Talal , AlEnizi Kholoud , Tleyjeh Imad
Published: August 2022

Seroprevalence of SARS-CoV-2 IgG among health care workers (HCWs) is crucial to inform infection control programs. Conflicting reports have emerged on the longevity of SARS-CoV-2 IgG. Our objective is to describe the prevalence of SARS-CoV-2 IgG in HCWs and perform 8 months longitudinal follow-up (FU) to assess the duration of detectable IgG. In addition, we aim to explore the risk factors associated with positive SARS-CoV-2 IgG. The study was conducted at a large COVID-19 public hospital in Riyadh, Saudi Arabia. All HCWs were recruited by social media platform. The SARS-CoV-2 IgG assay against SARS-CoV-2 nucleocapsid antigen was used. Multivariable logistic regression was used to examine association between IgG seropositive status and clinical and epidemiological factors. A total of 2528 (33% of the 7737 eligible HCWs) participated in the survey and 2523 underwent baseline serological testing in June 2020. The largest occupation groups sampled were nurses [n = 1351(18%)], physicians [n = 456 (6%)], administrators [n = 277 (3.6%)], allied HCWs [n = 205(3%)], pharmacists [n = 95(1.2%)], respiratory therapists [n = 40(0.5%)], infection control staff [n = 21(0.27%], and others [n = 83 (1%)]. The total cohort median age was 36 (31–43) years and 66.3% were females. 273 were IgG seropositive at baseline with a seroprevalence of 10.8% 95% CI (9.6%-12.1%). 165/185 and 44/112 were persistently IgG positive, at 2–3 months and 6 months FU respectively. The median (25th– 75th percentile) IgG level at the 3 different time points was 5.86 (3.57–7.04), 3.91 (2.46–5.38), 2.52 (1.80–3.99) respectively. Respiratory therapists OR 2.38, (P = 0.035), and those with hypertension OR = 1.86, (P = 0.009) were more likely to be seropositive. A high proportion of seropositive staff had prior symptoms 214/273(78%), prior anosmia was associated with the presence of antibodies, with an odds ratio of 9.25 (P<0.001), as well as fever and cough. Being a non-smoker, non-Saudi, and previously diagnosed with COVID-19 infection by PCR were statistically significantly different by seroprevalence status. We found that the seroprevalence of IgG against SARS-CoV-2 nucleocapsid antigen was 10.8% in HCWs at the peak of the pandemic in Saudi Arabia. We also observed a decreasing temporal trend of IgG seropositivity over 8 months follow up period.
Dental structure and tooth attachment modes in the common fangtooth Anoplogaster cornuta (Valenciennes, 1833) (Actinopterygii; Trachichthyiformes; Anoplogastridae)
Authors: Kierdorf Horst , Kierdorf Uwe , Greven Hartmut , Clemen Günter
Published: August 2022

We studied the structure and attachment modes of the teeth of adultAnoplogaster cornutausing light- and scanning-electron microscopic techniques. All teeth were monocuspid, composed solely of orthodentin, and lacked a covering enameloid cap. Fourteen teeth were present in the oral jaws, with three teeth each on the left and right premaxilla and four teeth each on the left and right dentary. The anteriormost premaxillary and dentary teeth were considerably larger than the more posteriorly located ones. The oral jaw teeth were transparent, non-depressible and firmly ankylosed to their respective dentigerous bone by a largely anosteocytic bone of attachment. No evidence for replacement of the large oral jaw teeth was found in the analyzed adult specimens. The bone of attachment exhibited lower calcium and phosphorus concentrations and a higher Ca/P ratio than the orthodentin. The connection between dentinal tooth shaft and bone of attachment was stabilized by a collar of mineralized collagen fibers. In contrast to the oral jaw teeth, the pharyngeal teeth exhibited a ring-like fibrous attachment to their supporting bones. This mode of attachment provides the teeth with some lateral mobility and allows their depression relative to their supporting bones, which may facilitate intra-pharyngeal prey transport. In contrast, a firm ankylosis was observed in numerous small teeth located on the branchial arches. The function of these teeth is presumably to increase the tightness of the pharyngeal basket and thereby the retention of small prey items in a species living in a habitat with only sparse food supply. Our findings corroborate earlier statements on the tooth attachment modes of the oral jaw teeth ofAnoplogaster cornuta, but provide new findings for the attachment modes of pharyngeal teeth in this species.
Association between HLA-C alleles and COVID-19 severity in a pilot study with a Spanish Mediterranean Caucasian cohort
Authors: Vigón Lorena , Galán Miguel , Torres Montserrat , Martín-Galiano Antonio J. , Rodríguez-Mora Sara , Mateos Elena , Corona Magdalena , Malo Rosa , Navarro Cristina , Murciano-Antón María Aránzazu , García-Gutiérrez Valentín , Planelles Vicente , Martínez-Laso Jorge , López-Huertas María Rosa , Coiras Mayte ,   on behalf of the Multidisciplinary Group of Study of COVID-19 (MGS-COVID)
Published: August 2022

The clinical presentations of COVID-19 may range from an asymptomatic or mild infection to a critical or fatal disease. Several host factors such as elderly age, male gender, and previous comorbidities seem to be involved in the most severe outcomes, but also an impaired immune response that causes a hyperinflammatory state but is unable to clear the infection. In order to get further understanding about this impaired immune response, we aimed to determine the association of specific HLA alleles with different clinical presentations of COVID-19. Therefore, we analyzed HLA Class I and II, as well as KIR gene sequences, in 72 individuals with Spanish Mediterranean Caucasian ethnicity who presented mild, severe, or critical COVID-19, according to their clinical characteristics and management. This cohort was recruited in Madrid (Spain) during the first and second pandemic waves between April and October 2020. There were no significant differences in HLA-A or HLA-B alleles among groups. However, despite the small sample size, we found that HLA-C alleles from group C1 HLA-C*08:02, -C*12:03, or -C*16:01 were more frequently associated in individuals with mild COVID-19 (43.8%) than in individuals with severe (8.3%; p = 0.0030; pc = 0.033) and critical (16.1%; p = 0.0014; pc = 0.0154) disease. C1 alleles are supposed to be highly efficient to present peptides to T cells, and HLA-C*12:03 may present a high number of verified epitopes from abundant SARS-CoV-2 proteins M, N, and S, thereby being allegedly able to trigger an efficient antiviral response. On the contrary, C2 alleles are usually poorly expressed on the cell surface due to low association with β2-microglobulin (β2M) and peptides, which may impede the adequate formation of stable HLA-C/β2M/peptide heterotrimers. Consequently, this pilot study described significant differences in the presence of specific HLA-C1 alleles in individuals with different clinical presentations of COVID-19, thereby suggesting that HLA haplotyping could be valuable to get further understanding in the underlying mechanisms of the impaired immune response during critical COVID-19.
COVID-19 vaccine acceptance among healthcare workers in China: A systematic review and meta-analysis
Authors: Shui Xiaoling , Wang Fang , Li Ling , Liang Qian
Published: August 2022

Background

Since the successful development of Coronavirus Disease (COVID-19) vaccine, COVID-19 vaccination has been actively advocated all over the world. As the key population for COVID-19 vaccination, the acceptance of Healthcare Workers (HCWs) is not only related to their risk of contracting COVID-19 infection at work, but also affects the decision of the general population on COVID-19 vaccination. Currently, a series of observational studies have been conducted on the acceptance of COVID-19 vaccines among HCWs in China, but there are presently no all-inclusive reviews. Therefore, this paper reviewed to identify a reliable estimate of acceptance rate of COVID-19 vaccine among HCWs in China.

Methods

We conducted a search on PubMed, EMbase, The Cochrane Library, Web of Science, CNKI (Chinese National Knowledge Infrastructure), Wanfang Database, CBM (Chinese Biomedical Literature Database) and VIP database (Chinese Scientific Journal Database) from January 2020 to June 2022. The quality of included articles was estimated using the Newcastle-Ottawa Quality Assessment tool suitable for cross-sectional studies and STATA 16 was used for analysis, A random-effects model was used to calculate acceptance rate for COVID-19 vaccine, as well as subgroup analysis and sensitivity analysis.

Result

This review included 18 studies involving 45,760 subjects, all of which were of medium or high quality. Meta-analysis results represented that, the pooled estimated acceptance rate of COVID-19 vaccine among HCWs in China was 78% (95%CI: 73–83%), and the pooled acceptance rate in 2021 (82%, 95%CI: 78–86%) was significantly higher than that in 2020 (73%, 95%CI: 65%-81%). Subgroup analysis showed different acceptance rates for COVID-19 vaccine among HCWs with different characteristics.

Conclusion

The result revealed that HCWs in China generally have a high acceptance rate of COVID-19 vaccines, but the acceptance rate varies with different characteristics of the population. Therefore, corresponding training should be carried out for HCWs with different characteristics, and they should play an exemplary and leading role in COVID-19 vaccination, so as to improve the vaccination rate of the whole population and form an immune barrier at an early date.
Malnutrition and maternal vaccination against typhoid toxin
Authors: Neupane Durga P. , Ahn Changhwan , Yang Yi-An , Lee Gi Young , Song Jeongmin
Published: August 2022

Children are particularly susceptible to typhoid fever caused by the bacterial pathogen Salmonella Typhi. Typhoid fever is prevalent in developing countries where diets can be less well-balanced. Here, using a murine model, we investigated the role of the macronutrient composition of the diet in maternal vaccination efficacies of two subunit vaccines targeting typhoid toxin: ToxoidVac and PltBVac. We found that maternal vaccinations protected all offspring against a lethal-dose typhoid toxin challenge in a balanced, normal diet (ND) condition, but the declined protection in a malnourished diet (MD) condition was observed in the PltBVac group. Despite the comparable antibody titers in both MD and ND mothers, MD offspring had a significantly lower level of typhoid toxin neutralizing antibodies than their ND counterparts. We observed a lower expression of the neonatal Fc receptor on the yolk sac of MD mothers than in ND mothers, agreeing with the observed lower antibody titers in MD offspring. Protein supplementation to MD diets, but not fat supplementation, increased FcRn expression and protected all MD offspring from the toxin challenge. Similarly, providing additional typhoid toxin-neutralizing antibodies to MD offspring was sufficient to protect all MD offspring from the toxin challenge. These results emphasize the significance of balanced/normal diets for a more effective maternal vaccination transfer to their offspring.
Evidencias complementarias de validez de la Escala Grit-S y adaptación de la Escala para medir la agencia y el empoderamiento en Colombia
Authors:   Universidad Católica de Colombia, Bogotá, Colombia, Uribe Moreno Miguel Eduardo , Castiblanco-Moreno Suelen Emilia ,   Universidad de La Salle, Bogotá, Colombia, Mejía-Vélez Susana ,   Universidad Católica de Colombia, Bogotá, Colombia, Guzmán Rincón Alfredo ,   Corporación Universitaria de Asturias, Bogotá, Colombia, Carrillo Barbosa Ruby Lorena ,   Corporación Universitaria de Asturias, Bogotá, Colombia, Medina-Arboleda Iván Felipe ,   Universidad Católica de Colombia, Bogotá, Colombia
Published: August 2022

Introducción: el constructo grit predice el desempeño en distintos dominios; aunque con resultados variables en la estructura de las pruebas. El objetivo de la investigación es aportar evidencias de validez de la Grit-S, a través de la confirmación de la estructura y la convergencia con la Escala para Medir Agencia Personal y Empoderamiento. Método: en primer lugar, estudiantes universitarios (n = 313) de modalidad virtual respondieron la prueba Grit-S y se efectuó un análisis factorial confirmatorio de la prueba. A continuación, se evaluó la convergencia con la Escala para Medir Agencia Personal y Empoderamiento. Resultados: Los hallazgos sugieren mantener la estructura bifactorial de Grit-S. Por otra parte, se adaptó la escala de agencia y empoderamiento a través de un análisis factorial exploratorio, con una estructura de cuatro factores que explican el 44.6 % de la varianza. A su vez, la correlación de las subdimensiones de Grit-S y la escala de agencia y empoderamiento son positivas. Conclusiones: La prueba Grit-S es válida y sensible a dimensiones de funcionamiento individual, por la convergencia con las subdimensiones de agencia y la baja relación con el empoderamiento social.
Control externo de la calidad en medicina del laboratorio. Avances y futuro
Authors: Ricós Carmen , Fernández-Calle Pilar , Perich Carmen , Sandberg Sverre
Published: August 2022

Objectivos

Un programa de control externo distribuye las mismas muestras control entre varios laboratorios y evalúa los resultados obtenidos con un criterio común. El objetivo de este trabajo es resumir la evolución de los programas externos, poner de manifiesto los progresos conseguidos y deducir consecuencias prácticas para el laboratorio participante.

Métodos

El material es una breve revisión de los diferentes tipos de programas externos utilizados a lo largo de cuarenta años. El método es el análisis crítico de las ventajas e inconvenientes de cada modelo, a la luz de nuestra experiencia.

Resultados

A mitad del siglo XX se iniciaron los programas EQA, detectándose gran discrepancia entre resultados emitidos por distintos laboratorios. Se desarrollaron EQA en muchos países y se propusieron mecanismos para armonizarlos, como: establecer especificaciones derivadas de la variación biológica, promover el uso de métodos analíticos homogéneos, usar el EQA como herramienta educacional. A partir del 2000 se hacen importantes avances: asegurar el adecuado uso clínico de las pruebas del laboratorio, utilizar material control conmutable con el espécimen humano, armonizar los distintos modelos de EQA, promover una organización de cooperación entre organizadores de programas EQA.

Conclusiones

Participar en un EQA con controles conmutables y valores asignados por método de referencia certificado permite conocer la inexactitud real de los resultados y el impacto en las muestras de pacientes. Si se participa en programas con controles no conmutables solo se conoce si la prestación del laboratorio es similar a la de otros usuarios del mismo método analítico.
Biomagnetic signals recorded during transcranial magnetic stimulation (TMS)-evoked peripheral muscular activity
Authors: Iwata Geoffrey Z. , Hu Yinan , Wickenbrock Arne , Sander Tilmann , Muthuraman Muthuraman , Chirumamilla Venkata Chaitanya , Groppa Sergiu , Liu Qishan , Budker Dmitry
Published: August 2022

Transcranial magnetic stimulation (TMS) has widespread clinical applications from diagnosis to treatment. We combined TMS with non-contact magnetic detection of TMS-evoked muscle activity in peripheral limbs to explore a new diagnostic modality that enhances the utility of TMS as a clinical tool by leveraging technological advances in magnetometry. We recorded measurements in a regular hospital room using an array of optically pumped magnetometers (OPMs) inside a portable shield that encloses only the forearm and hand of the subject. We present magnetomyograms (MMG)s of TMS-evoked movement in a human hand, together with a simultaneous surface electromyograph (EMG) data. The biomagnetic signals recorded in the MMG provides detailed spatial and temporal information that is complementary to that of the electric signal channels. Moreover, we identify features in the magnetic recording beyond that of the EMG. This system demonstrates the value of biomagnetic signals in TMS-based clinical approaches and widens its availability and practical potential.
Low-temperature corrosion behavior of laser cladding metal-based alloy coatings on EH40 high-strength steel for icebreaker
Authors: Wang Xinwang , Fan Li , Xu Yurong , Chen Haiyan , Cao Qizheng , Dong Lihua , Qin Yujiang
Published: August 2022

In this paper, four kinds of coatings Ni + 30% WC, Ni + 15% WC, Ni, and Co are prepared on EH40 steel by laser cladding technology. Electrochemical experiment tests at a simulating low-temperature (–40°C) environment in 0.5 mol·L−1 hydrochloric acid (HCl) solution and 3.5 wt% NaCl solution were conducted, and the polarization curve and electrochemical impedance spectroscopy (EIS) impedance spectrum were obtained. The results show that the Ni + 15% WC coating has the smallest corrosion current and 0.5 mol·L−1 HCl solution and 3.5 wt% NaCl solution have the maximum polarization resistance and impedance arc. Coatings will be treated in a 0.5 mol·L−1 HCl solution and 3.5 wt% NaCl solution at –40°C immersion for 30 days; finally, X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM) were used for test passivation films of Ni + 15% WC samples and sample surface morphology after corrosion. XPS results show that Fe, Mo, and Ni elements can form metal oxides in the 0.5 mol·L−1 HCl solution, and Cr, Fe, Mo, Ni, and W elements can form metal oxides in the 3.5 wt% NaCl solution. SEM results display that relatively severe corrosion appeared on the substrate near tungsten carbide in a 0.5 mol·L−1 HCl solution, and coating will be corroded form inside of the coating owing to the corrosive solution will penetrate the substrate. In 3.5 wt% NaCl solution, severe corrosion of the substrate has not been observed; however, the binding part has been obviously corroded.
Non-invasive prenatal screening tests – update 2022
Authors: Kypri Elena , Ioannides Marios , Achilleos Achilleas , Koumbaris George , Patsalis Philippos , Stumm Markus
Published: August 2022

Since 2012, non-invasive prenatal testing (NIPT) using cell-free DNA from maternal plasma is applied all over the world as highly efficient first-line or contingent screening approach for trisomy 13, 18 and 21. With further technical development the screening has expanded to other genetic conditions such as sex chromosome anomalies (SCAs), rare autosomal trisomies (RATs), microdeletions/microduplications, structural chromosomal aberrations and monogenic diseases. Meanwhile, commercial providers are offering a number of different tests, with variable performance, the application of which needs to be carefully evaluated to apply to the true needs of clinical practice. In our review we present the different NIPT methodologies and discuss the main strengths and limitations in the context of providing a responsible pregnancy management.
Dancing all the way to the stage by way of the stadium: on the iconicity and plasticity of actions
Authors: Sonesson Göran
Published: August 2022

In the sense of phenomenology, actions are special cases of acts of consciousness. Within semiotics, first Jan Mukařovský and then A. J. Greimas have established, in different terms, a distinction between instrumental actions and actions which carry their meaning in themselves. But this is insufficient to account for the variety of actions which comprises everything from the creation of artefacts, dance, sporting events, theatre, rituals, and much else. Already those actions mentioned relate in different ways to instrumentality and intrinsic meaning, as well as to (depictive) iconicity and plasticity. In this paper, I will be particularly concerned with dancing, especially classical ballet, trying to delimit it from sporting events and theatre. Apart from the sign function and the spectacular function, I will notably have recourse to what I have elsewhere term secondary (depictive) iconicity as well as to plasticity. In the process, I hope to be able to throw some light also on the nature of sport, by comparing and contrasting it to dance, as well as to the theatre as well as, more incidentally, to some other types of action. In a wider perspective, my ambition is to contribute to a general inquiry into the semiosis of actions.
Qualitative behavior of a discrete predator–prey system under fear effects
Authors: Din Qamar , Zulfiqar Muhammad Arfan
Published: August 2022

Numerous field data and experiments on the perching birds or songbirds show that the fear of predators can cause significant changes in the prey population. Fear of predatory populations increases the chances of survival of the prey population, and this can greatly reduce the reproduction of the prey population. The influence of fear has contributed a leading role in both the environmental biology and theoretical ecology. Taking into account the interaction of predator–prey with non-overlapping generations, a discrete-time model is proposed and studied. Keeping in mind the biological feasibility of species, the existence of fixed points is studied along with the local asymptotic behavior of the proposed model around these fixed points. Furthermore, taking into account the oscillatory behavior of the model, various types of bifurcations are analyzed about biologically feasible fixed points with an application of center manifold theory and bifurcation theory of normal forms. Existence of chaos is discussed, and fluctuating and chaotic behavior of the system is controlled through implementation of different chaos control procedures. The illustration of theoretical discussion is carried out via validation of observed experimental field data and appropriate numerical simulation.
Synthesis, structures, and photophysical properties of two Cu(I) complexes supported by N-heterocyclic carbene and phosphine ligands
Authors: Lou Xinhua , Tian Yunfei , Wang Zhiqiang
Published: August 2022

Two new cationic four-coordinate Cu(I) complexes supported by different chelating N-heterocyclic carbene ligands and the diphosphine ligand bis[2-(diphenylphosphino)phenyl]ether (POP) have been synthesized. The chemical structures of both complexes have been characterized by 1H NMR, 13C NMR, 31P NMR, and mass spectroscopy, and the crystal structure of one complex has been determined by single-crystal X-ray diffraction. Results of theoretical calculations indicate that the lowest energy electronic transitions of these complexes are mainly the metal-to-ligand charge transfer and ligand-to-ligand charge transfer transitions. The complexes in solid state show intense emissions with high photoluminescence quantum yields. The photophysical behavior at 298 and 77K shows that emissions of these complexes at room temperature are thermally activated delayed fluorescence mixed with phosphorescence.
La Comuna en México: disputa entre liberales moderados y republicanos demócratas a partir de la experiencia revolucionaria parisina
Authors: Campos Pérez Lara
Published: August 2022

Este artículo analiza el debate político que se desarrolló en México en el verano de 1871 originado en los sucesos de la Comuna de París ocurrida ese mismo año. En el debate tomaron parte representantes de dos segmentos del arco ideológico: liberales, sobre todo en su ala moderada, y un grupo de demócratas republicanos partidarios del federalismo y del autogobierno. Discuto la forma en que ambos segmentos de la opinión pública construyeron sus discursos y esbozaron sus respectivos modelos republicanos, utilizando la Comuna parisina como espejo de sus reflexiones y como marco para la definición de sus posturas. Desde un enfoque de la historia de las ideas políticas, el artículo busca mostrar la riqueza y complejidad del pensamiento político mexicano durante este periodo.
The Impact of Covid-19 Outbreak on Banking Policies in Indonesia
Authors: Ganefi Ganefi , Hatikasari Siti
Published: August 2022

This research is descriptive analysis and uses a statutory approach. This research also uses qualitative juridical analysis which is conducted by using inductive deductive thinking method and vice versa. Conducted to find out and describe the corona virus impact on regulation in banking sector, and how the Financial Services Authority’s role for protecting banking consumers affected by Covid-19. The impact of Covid-19 has an economic activities impact, including in banking sector, which causes debtors to experience difficulties in carrying out achievements. Although, the government issued several policies including the existence of regulations regarding State Financial Policy and Financial System Stability for Handling the Corona Virus Disease 2019 Pandemic or in the Context of Facing Threats that Endanger the National Economy and Financial System Stability. Furthermore, the Financial Services Authority as a banking supervisory institution also took part in and supported the Government's policies, namely by issuing several policies in order to protect customers affected by Covid-19, through its policies, banking institutions are required to implement a credit relaxation program like credit restructuring for affected customers.
Physical Activity and Nutrition Interventions for Type 1 Diabetes: A Meta-analysis
Authors: Shorey Shefaly , Ng Esperanza D. , Law Evelyn C. , Wong John C.M. , Loke Kah Yin , Tam Wilson W.S.
Published: August 2022

BACKGROUND AND OBJECTIVES

Current evidence is lacking on physical activity and nutrition-based interventions focusing on the management of type 1 diabetes mellitus (T1DM) and health-related quality of life among children. To assess the effects of physical activity interventions and nutrition-based interventions for children with T1DM.

METHODS

Data sources include the Cochrane Central Register of Controlled Trials, Medline, clinicaltrials.gov, the World Health Organization International Clinical Trials Registry Platform, CINAHL through January 2022. Study selection includes randomized controlled trials of children aged 18 years and below with T1DM comparing either a physical activity intervention, a nutrition-based intervention, or hybrid physical activity and nutrition-based intervention with placebo or no-treatment control. Data were pooled using a random-effects model. Primary outcomes were hemoglobin A1c (HbA1c), and health-related quality of life.

RESULTS

Eighteen trials were included. Physical activity compared with the no-treatment group showed a lack of effect on HbA1c (mean difference = −0.58, 95% confidence interval −1.20 to 0.05; P value = .07). Nutrition-based intervention compared with no-treatment control for HbA1c level revealed a lack of effect (mean difference = −0.61, 95% confidence interval −1.48 to 0.26; P value = .17). Limitations include paucity of studies and low quality of evidence caused by the risk of bias.

CONCLUSIONS

Despite the lack of significant evidence, the generally favorable results highlight the potential of such interventions in enhancing glycemic control and health-related quality of life. Additionally, promising results from a single physical activity-nutrition-based hybrid intervention in terms of glycemic control indicate the plausible effectiveness of a mixed intervention.