bluuebunny/crossref_metadata_2025 huggingface dataset:
A Fur family protein BosR is a novel RNA-binding protein that controls rpoS RNA stability in the Lyme disease pathogen
Authors: Raghunandanan Sajith , Priya Raj , Alanazi Fuad , Lybecker Meghan C , Schlax Paula Jean , Yang X Frank
Published: February 2024

The σ54-σS sigma factor cascade plays a central role in regulating differential gene expression during the enzootic cycle of Borreliella burgdorferi, the Lyme disease pathogen. In this pathway, the primary transcription of rpoS (which encodes σS) is under the control of σ54 which is activated by a bacterial enhancer-binding protein (EBP), Rrp2. The σ54-dependent activation in B. burgdorferi has long been thought to be unique, requiring an additional factor, BosR, a homologue of classical Fur/PerR repressor/activator. However, how BosR is involved in this σ54-dependent activation remains unclear and perplexing. In this study, we demonstrate that BosR does not function as a regulator for rpoS transcriptional activation. Instead, it functions as a novel RNA-binding protein that governs the turnover rate of rpoS mRNA. We further show that BosR directly binds to the 5′ untranslated region (UTR) of rpoS mRNA, and the binding region overlaps with a region required for rpoS mRNA degradation. Mutations within this 5′UTR region result in BosR-independent RpoS production. Collectively, these results uncover a novel role of Fur/PerR family regulators as RNA-binding proteins and redefine the paradigm of the σ54–σS pathway in B. burgdorferi.
Nuclear RNA-related processes modulate the assembly of cytoplasmic RNA granules
Authors: Angel Mor , Fleshler Eden , Atrash Mohammad Khaled , Kinor Noa , Benichou Jennifer I C , Shav-Tal Yaron
Published: February 2024

Stress granules (SGs) are cytoplasmic assemblies formed under various stress conditions as a consequence of translation arrest. SGs contain RNA-binding proteins, ribosomal subunits and messenger RNAs (mRNAs). It is well known that mRNAs contribute to SG formation; however, the connection between SG assembly and nuclear processes that involve mRNAs is not well established. Here, we examine the effects of inhibiting mRNA transcription, splicing and export on the assembly of SGs and the related cytoplasmic P body (PB). We demonstrate that inhibition of mRNA transcription, splicing and export reduces the formation of canonical SGs in a eukaryotic initiation factor 2α phosphorylation-independent manner, and alters PB size and quantity. We find that the splicing inhibitor madrasin promotes the assembly of stress-like granules. We show that the addition of synthetic mRNAs directly to the cytoplasm is sufficient for SG assembly, and that the assembly of these SGs requires the activation of stress-associated protein synthesis pathways. Moreover, we show that adding an excess of mRNA to cells that do not have active splicing, and therefore have low levels of cytoplasmic mRNAs, promotes SG formation under stress conditions. These findings emphasize the importance of the cytoplasmic abundance of newly transcribed mRNAs in the assembly of SGs.
Preclinical evaluation of the third-generation, bi-steric mechanistic target of rapamycin complex 1-selective inhibitor RMC-6272 in NF2-deficient models
Authors: Bhattacharyya Srirupa , Oblinger Janet L , Beauchamp Roberta L , Kosa Lili , Robert Francis , Plotkin Scott R , Chang Long-Sheng , Ramesh Vijaya
Published: February 2024

Background

NF2-associated meningiomas are progressive, highly morbid, and nonresponsive to chemotherapies, highlighting the need for improved treatments. We have established aberrant activation of the mechanistic target of rapamycin (mTOR) signaling in NF2-deficient tumors, leading to clinical trials with first- and second-generation mTOR inhibitors. However, results have been mixed, showing stabilized tumor growth without shrinkage offset by adverse side effects. To address these limitations, here we explored the potential of third-generation, bi-steric mTOR complex 1 (mTORC1) inhibitors using the preclinical tool compound RMC-6272.

Methods

Employing human NF2-deficient meningioma lines, we compared mTOR inhibitors rapamycin (first-generation), INK128 (second-generation), and RMC-6272 (third-generation) using in vitro dose–response testing, cell-cycle analysis, and immunoblotting. Furthermore, the efficacy of RMC-6272 was assessed in NF2-null 3D-spheroid meningioma models, and its in vivo potential was evaluated in 2 orthotopic meningioma mouse models.

Results

Treatment of meningioma cells revealed that, unlike rapamycin, RMC-6272 demonstrated superior growth inhibitory effects, cell-cycle arrest, and complete inhibition of phosphorylated 4E-BP1 (mTORC1 readout). Moreover, RMC-6272 had a longer retention time than INK128 and inhibited the expression of several eIF4E-sensitive targets on the protein level. RMC-6272 treatment of NF2 spheroids showed significant shrinkage in size as well as reduced proliferation. Furthermore, in vivo studies in mice revealed effective blockage of meningioma growth by RMC-6272, compared with vehicle controls.

Conclusions

Our study in preclinical models of NF2 supports possible future clinical evaluation of third-generation, investigational mTORC1 inhibitors, such as RMC-5552, as a potential treatment strategy for NF2.
The Effectiveness of Text Support for Stopping Smoking in Pregnancy (MiQuit): Multi-Trial Pooled Analysis Investigating Effect Moderators and Mechanisms of Action
Authors: Emery Joanne , Leonardi-Bee Jo , Coleman Tim , McDaid Lisa , Naughton Felix
Published: February 2024

Introduction

Digital cessation support appeals to pregnant smokers. In two pooled RCTs, MiQuit, a pregnancy-specific tailored text messaging intervention, did not show effectiveness for validated prolonged abstinence. However, secondary outcomes and potential moderators and mediators have not been investigated. We aimed to determine, using pooled RCT data: (1) MiQuit effectiveness on a range of smoking outcomes; (2) whether baseline tobacco dependence or quit motivation moderate effectiveness; (3) whether hypothesized mechanisms of action (quitting determination, self-efficacy, baby harm beliefs, lapse prevention strategies) mediate effectiveness.

Methods

Pooled data analysis from two procedurally identical RCTs comparing MiQuit (N = 704) to usual care (N = 705). Participants were smokers, <25 weeks pregnant, recruited from 40 English antenatal clinics. Outcomes included self-reported 7-day abstinence at 4 weeks post-baseline and late pregnancy, and prolonged abstinence. Late pregnancy outcomes were also biochemically validated. We used hierarchical regression and structural equation modeling.

Results

MiQuit increased self-reported, 7-day abstinence at 4 weeks (OR = 1.73 [95% CI 1.10–2.74]) and was borderline significant at late pregnancy (OR = 1.34 [0.99–1.82]) but not for prolonged or validated outcomes. Effectiveness was not moderated by baseline dependence (heaviness of smoking “low” vs. “moderate–high”) or motivation (planning to quit ≤30 days [high] vs. >30 days [low]), but effects on self-reported outcomes were larger for the high motivation sub-group. MiQuit had a small effect on mean lapse prevention strategies (MiQuit 8.6 [SE 0.17], UC 8.1 [SE 0.17]; P = .030) but not other mechanisms.

Conclusions

MiQuit increased short-term but not prolonged or validated abstinence and may be most effective for those motivated to quit sooner.

Implications

Digital cessation support appeals to pregnant smokers. MiQuit, a tailored, theory-guided text messaging program for quitting smoking in pregnancy, has not shown effectiveness for validated prolonged abstinence in two previous RCTs but its impact on other smoking outcomes and potential mechanisms of action are unknown. When pooling trial data, MiQuit increased self-reported short-term abstinence, including making a quit attempt and abstinence at 4-week follow-up, but not late pregnancy, sustained, or validated abstinence. MiQuit appeared effective at late pregnancy for participants with high quitting motivation, but its mechanisms of action remain uncertain. Additional support components are likely required to enhance effectiveness.
Nutritional interventions in prison settings: a scoping review
Authors: Vetrani Claudia , Verde Ludovica , Ambretti Antinea , Muscogiuri Giovanna , Pagano Antonio Maria , Lucania Luciano , Colao Annamaria , Barrea Luigi
Published: February 2024

Background

Mounting evidence has shown that incarceration can affect the health and well-being of individuals and increase the risk of noncommunicable diseases (NCDs). Diet quality is known to be one of the main determinants of risk of NCDs, and dietary changes are the first approach used in primary care to reduce the incidence of NCDs.

Objective

This scoping review aimed to summarize the evidence for (1) the diet quality of inmates, and (2) the effect of nutritional intervention in prison systems. In addition, we aimed to describe limitations in the current literature and to suggest potential future research areas.

Method

A systematic search was performed in 2 databases (PubMed and Web of Science) using predefined search terms and covering the period May 2023 to June 2023. Additionally, reference lists from the retrieved studies were hand-searched to identify any additional relevant publications. The identified literature was screened based on defined search strategies, criteria, and research questions defined using the PICo (population or problem, interest, and context) framework. The review was conducted referring to the PRISMA-ScR and the PICo framework.

Results

A total of 19 studies out of 63 initially identified records were included in this review (11 cross-sectional evaluations and 9 intervention-based studies). In almost all studies, assessment of the diet quality of menus showed the menus to be nutritionally adequate, except for having a higher-than-recommended intake of total energy, saturated fatty acids, sodium, cholesterol, and sugar. In addition, some studies reported a lower-than-recommended intake of fiber, magnesium, potassium, vitamins D, E, and A, and omega-3 fatty acids. Nutritional interventions were mainly planned in the form of workshops, seminars, and written material to deliver information on healthy dietary choices. Although no significant changes in inmates’ dietary choices were observed in any of the studies, a high participation rate was detected.

Conclusion

Inmates might require additional prevention intervention to reduce their susceptibility to cardiometabolic diseases by virtue of their isolation from community facilities. Interventions should be tailored to the characteristics of prison settings and inmates to increase adherence to nutritional recommendations.
Hypoxemic Respiratory Failure and Coccidioidomycosis-Associated Acute Respiratory Distress Syndrome
Authors: Heidari Arash , Kaur Simmer , Pearson Skyler J , Munoz Augustine , Sandhu Harleen , Mann Gursimran , Schivo Michael , Zeki Amir A , Bays Derek J , Wilson Machelle , Albertson Timothy E , Johnson Royce , Thompson George R
Published: February 2024

Background

Severe coccidioidomycosis presenting with respiratory failure is an uncommon manifestation of disease. Current knowledge of this condition is limited to case reports and small case series.

Methods

A retrospective multicenter review of patients with coccidioidomycosis-associated acute respiratory distress syndrome (CA-ARDS) was conducted. It assessed clinical and laboratory variables at the time of presentation, reviewed the treatment course, and compared this cohort with a national database of patients with noncoccidioidomycosis ARDS. Survivors and nonsurvivors of coccidioidomycosis were also compared to determine prognostic factors.

Results

In this study, CA-ARDS (n = 54) was most common in males, those of Hispanic ethnicity, and those with concurrent diabetes mellitus. As compared with the PETAL network database (Prevention and Early Treatment of Acute Lung Injury; n = 1006), patients with coccidioidomycosis were younger, had fewer comorbid conditions, and were less acidemic. The 90-day mortality was 15.4% for patients with coccidioidomycosis, as opposed to 42.6% (P < .0001) for patients with noncoccidioidomycosis ARDS. Patients with coccidioidomycosis who died, as compared with those who survived, were older, had higher APACHE II scores (Acute Physiology and Chronic Health Evaluation), and did not receive corticosteroid therapy.

Conclusions

CA-ARDS is an uncommon but morbid manifestation of infection. When compared with a national database, the overall mortality appears favorable vs other causes of ARDS. Patients with CA-ARDS had a low overall mortality but required prolonged antifungal therapy. The utility of corticosteroids in this condition remains unconfirmed.
The Burden and Impact of Early Post-transplant Multidrug-Resistant Organism Detection Among Renal Transplant Recipients, 2005–2021
Authors: Babiker Ahmed , Karadkhele Geeta , Bombin Andrei , Watkins Rockford , Robichaux Chad , Smith Gillian , Beechar Vivek B , Steed Danielle B , Jacob Jesse T , Read Timothy D , Satola Sarah , Larsen Christian P , Kraft Colleen S , Pouch Stephanie M , Woodworth Michael H
Published: February 2024

Background

Reducing the burden of multidrug-resistant organism (MDRO) colonization and infection among renal transplant recipients (RTRs) may improve patient outcomes. We aimed to assess whether the detection of an MDRO or a comparable antibiotic-susceptible organism (CSO) during the early post-transplant (EPT) period was associated with graft loss and mortality among RTRs.

Methods

We conducted a retrospective cohort study of RTRs transplanted between 2005 and 2021. EPT positivity was defined as a positive bacterial culture within 30 days of transplant. The incidence and prevalence of EPT MDRO detection were calculated. The primary outcome was a composite of 1-year allograft loss or mortality following transplant. Multivariable Cox hazard regression, competing risk, propensity score–weighted sensitivity, and subgroup analyses were performed.

Results

Among 3507 RTRs, the prevalence of EPT MDRO detection was 1.3% (95% CI, 0.91%–1.69%) with an incidence rate per 1000 EPT-days at risk of 0.42 (95% CI, 0.31–0.57). Among RTRs who met survival analysis inclusion criteria (n = 3432), 91% (3138/3432) had no positive EPT cultures and were designated as negative controls, 8% (263/3432) had a CSO detected, and 1% (31/3432) had an MDRO detected in the EPT period. EPT MDRO detection was associated with the composite outcome (adjusted hazard ratio [aHR], 3.29; 95% CI, 1.21–8.92) and death-censored allograft loss (cause-specific aHR, 7.15; 95% CI, 0.92–55.5; subdistribution aHR, 7.15; 95% CI, 0.95–53.7). A similar trend was seen in the subgroup and sensitivity analyses.

Conclusions

MDRO detection during the EPT period was associated with allograft loss, suggesting the need for increased strategies to optimize prevention of MDRO colonization and infection.
Implementation of a Prospective Index-Cluster Sampling Strategy for the Detection of Presymptomatic Viral Respiratory Infection in Undergraduate Students
Authors: Uthappa Diya M , McClain Micah T , Nicholson Bradly P , Park Lawrence P , Zhbannikov Ilya , Suchindran Sunil , Jimenez Monica , Constantine Florica J , Nichols Marshall , Jones Daphne C , Hudson Lori L , Jaggers L Brett , Veldman Timothy , Burke Thomas W , Tsalik Ephraim L , Ginsburg Geoffrey S , Woods Christopher W
Published: February 2024

Background

Index-cluster studies may help characterize the spread of communicable infections in the presymptomatic state. We describe a prospective index-cluster sampling strategy (ICSS) to detect presymptomatic respiratory viral illness and its implementation in a college population.

Methods

We enrolled an annual cohort of first-year undergraduates who completed daily electronic symptom diaries to identify index cases (ICs) with respiratory illness. Investigators then selected 5–10 potentially exposed, asymptomatic close contacts (CCs) who were geographically co-located to follow for infections. Symptoms and nasopharyngeal samples were collected for 5 days. Logistic regression model–based predictions for proportions of self-reported illness were compared graphically for the whole cohort sampling group and the CC group.

Results

We enrolled 1379 participants between 2009 and 2015, including 288 ICs and 882 CCs. The median number of CCs per IC was 6 (interquartile range, 3–8). Among the 882 CCs, 111 (13%) developed acute respiratory illnesses. Viral etiology testing in 246 ICs (85%) and 719 CCs (82%) identified a pathogen in 57% of ICs and 15% of CCs. Among those with detectable virus, rhinovirus was the most common (IC: 18%; CC: 6%) followed by coxsackievirus/echovirus (IC: 11%; CC: 4%). Among 106 CCs with a detected virus, only 18% had the same virus as their associated IC. Graphically, CCs did not have a higher frequency of self-reported illness relative to the whole cohort sampling group.

Conclusions

Establishing clusters by geographic proximity did not enrich for cases of viral transmission, suggesting that ICSS may be a less effective strategy to detect spread of respiratory infection.
An unusual presentation of takotsubo syndrome in an elderly patient
Authors: Khan Israr , Atoot Adam
Published: February 2024

Takotsubo syndrome occurs predominantly in women and is usually preceded by acute emotional and/or physical stress. Patients commonly present with chest pain and/or dyspnea. Syncope or out-of-hospital cardiac arrest is a rare presentation. We report an unusual case of takotsubo syndrome in an elderly patient who presented with left facial droop, slurred speech, and syncopal episode. Initial presumed diagnosis was an ischemic stroke/transient ischemic attack. However, the patient was then treated for acute myocardial ischemia/infarction based on abnormal electrocardiogram, elevated cardiac troponin, and unremarkable neurological workup. Eventually diagnosed with takotsubo syndrome. Our case illustrates the importance of prompt identification particularly in the context of atypical presentation and further evaluation to rule out serious causes to mitigate related morbidity and mortality while simultaneously avoiding unnecessary investigations.
The sad tale of a youthful right ventricle struggling with endomyocardial fibrosis
Authors: El-Mhadi Samah , Agoumy Zineb , El Hajjaj Belghait , Bendagha Nesma , Soufiani Aida , Moughil Said
Published: February 2024

Endomyocardial fibrosis (EMF) is a rare and often underdiagnosed form of restrictive cardiomyopathy. Prognosis is generally unfavorable. Early diagnosis, along with surgical and medical intervention, is crucial for improved outcomes. We report the case of a 15-year-old girl from Guinea who presented with suspected Ebstein’s anomaly and severe right heart failure. Multimodal cardiac imaging revealed right ventricular EMF. Despite counseling on prognosis, the family declined surgery. The patient experienced cardiac arrest two months later.
Patient with a history of Glanzmann thrombasthenia presented with chronic subdural hematoma: a case report study
Authors: Hasanpour Mohammad , Mehdizadeh Hajar
Published: February 2024

Glanzmann thrombasthenia (GT) is a rare platelet disorder characterized by qualitative/quantitative deficiencies of the platelets’ fibrinogen receptor, glycoprotein (GP) IIb/IIIa complex, resulting in impaired platelet aggregation and increased bleeding time. Most cases are hereditary with an autosomal recessive pattern of inheritance, but acquired GT also occurs. We report the surgical management of symptomatic chronic subdural hematoma (CSDH), a rare condition in young individuals, in a 37-year-old man who had GT and a history of mild head trauma approximately one month before admission. Despite hematologic consultation, normal bleeding time and clotting time, and platelet transfusion before surgery, massive hemorrhage during surgery, epidural hematoma, and anisocoria in the ICU occurred that led to craniectomy. This report highlights that CSDH management in patients with GT requires close monitoring of these patients as well as collaboration between neurosurgeons, intensive care physicians, hematologists, and anesthesiologists.
Effective sympathetic nerve block for chemotherapy-induced peripheral neuropathy: a case report
Authors: Kuroyanagi Aya , Inano Chiaki , Adachi Junko , Kaneko Goro , Toyokawa Hideki
Published: February 2024

Chemotherapy-induced peripheral neuropathy (CIPN) is one of the most difficult-to-alleviate side effects of chemotherapy, impacting the patient’s daily activities and quality of life and frequently necessitating the discontinuation or dose reduction of anticancer drugs. An effective treatment for CIPN is yet to be established. Herein, we report the case of a patient who developed CIPN after receiving paclitaxel as postoperative chemotherapy for breast cancer. The patient experienced difficulties in performing daily activities owing to pain in her fingers and toes despite attempts to treat these symptoms with medications. Stellate and lumbar sympathetic ganglion blocks improved CIPN-induced symptoms of numbness and pain in the extremities. Thereafter, lumbar sympathetic ganglion block was performed once every 6 months, markedly improving the patient’s quality of life. Accordingly, sympathetic nerve block can facilitate pain control in patients with CIPN refractory to pharmacotherapy.
Education gradient in differential health reporting error among older adults in India
Authors: Choi Anna , Basu Arnab K , Chau Nancy H , Sekher T V
Published: February 2024

This paper studies the education gradient associated with health reporting errors for two highly prevalent non-communicable diseases among older adults in India. We analyze a novel data set—the Longitudinal Aging Study in India (2017–18) panel survey—to unpack the sources of health reporting error in a developing-country context for the first time. Our analysis points to a statistically significant level of false negative reporting (or over-reporting) for both high blood pressure and depression by those with no schooling and less than middle school education relative to their more highly educated peers. Interestingly, this result is driven by those whose income is above the poverty line. We further find false negative reporting for high blood pressure to be more prevalent among women with no schooling.
Secondary Amazon rainforest partially recovers tree cavities suitable for nesting birds in 18–34 years
Authors: Dantas Oliveira Carine , Cornelius Cintia , Stouffer Philip C , Cockle Kristina L
Published: February 2024

Passive restoration of secondary forests can partially offset loss of biodiversity following tropical deforestation. Tree cavities, an essential resource for cavity-nesting birds, are usually associated with old forest. We investigated the restoration time for tree cavities suitable for cavity-nesting birds in secondary forest at the Biological Dynamics of Forest Fragments Project (BDFFP) in central Amazonian Brazil. We hypothesized that cavity abundance would increase with forest age, but more rapidly in areas exposed to cutting only, compared to areas where forest was cut and burned. We also hypothesized that cavities would be lower, smaller, and less variable in secondary forest than in old-growth forest, which at the BDFFP is part of a vast lowland forest with no recent history of human disturbance. We used pole-mounted cameras and tree-climbing to survey cavities in 39 plots (each 200 × 40 m) across old-growth forests and 11–34-year-old secondary forests. We used generalized linear models to examine how cavity supply was related to forest age and land-use history (cut only vs cut-and-burn), and principal components analysis to compare cavity characteristics between old-growth and secondary forest. Cavity availability increased with secondary forest age, regardless of land-use history, but the oldest secondary forest (31–34 years) still had fewer cavities (mean ± SE = 9.8 ± 2.2 cavities ha–1) than old-growth forest (20.5 ± 4.2 cavities ha–1). Moreover, secondary forests lacked cavities that were high and deep, with large entrances—characteristics likely to be important for many species of cavity-nesting birds. Several decades may be necessary to restore cavity supply in secondary Amazonian forests, especially for the largest birds (e.g., forest-falcons and parrots > 190 g). Retention of legacy trees as forest is cleared might help maintain a supply of cavities that could allow earlier recolonization by some species of cavity-nesting birds when cleared areas are abandoned. A Portuguese version of this article is available in Supplementary Material 1.
Nitrogen sensing and regulatory networks: it's about time and space
Authors: Shanks Carly M , Rothkegel Karin , Brooks Matthew D , Cheng Chia-Yi , Alvarez José M , Ruffel Sandrine , Krouk Gabriel , Gutiérrez Rodrigo A , Coruzzi Gloria M
Published: February 2024

A plant's response to external and internal nitrogen signals/status relies on sensing and signaling mechanisms that operate across spatial and temporal dimensions. From a comprehensive systems biology perspective, this involves integrating nitrogen responses in different cell types and over long distances to ensure organ coordination in real time and yield practical applications. In this prospective review, we focus on novel aspects of nitrogen (N) sensing/signaling uncovered using temporal and spatial systems biology approaches, largely in the model Arabidopsis. The temporal aspects span: transcriptional responses to N-dose mediated by Michaelis-Menten kinetics, the role of the master NLP7 transcription factor as a nitrate sensor, its nitrate-dependent TF nuclear retention, its “hit-and-run” mode of target gene regulation, and temporal transcriptional cascade identified by “network walking.” Spatial aspects of N-sensing/signaling have been uncovered in cell type-specific studies in roots and in root-to-shoot communication. We explore new approaches using single-cell sequencing data, trajectory inference, and pseudotime analysis as well as machine learning and artificial intelligence approaches. Finally, unveiling the mechanisms underlying the spatial dynamics of nitrogen sensing/signaling networks across species from model to crop could pave the way for translational studies to improve nitrogen-use efficiency in crops. Such outcomes could potentially reduce the detrimental effects of excessive fertilizer usage on groundwater pollution and greenhouse gas emissions.
MAC3A and MAC3B mediate degradation of the transcription factor ERF13 and thus promote lateral root emergence
Authors: Yu Zipeng , Qu Xingzhen , Lv Bingsheng , Li Xiaoxuan , Sui Jiaxuan , Yu Qianqian , Ding Zhaojun
Published: February 2024

Lateral roots (LRs) increase root surface area and allow plants greater access to soil water and nutrients. LR formation is tightly regulated by the phytohormone auxin. Whereas the transcription factor ETHYLENE-RESPONSIVE ELEMENT BINDING FACTOR13 (ERF13) prevents LR emergence in Arabidopsis (Arabidopsis thaliana), auxin activates MITOGEN-ACTIVATED PROTEIN KINASE14 (MPK14), which leads to ERF13 degradation and ultimately promotes LR emergence. In this study, we discovered interactions between ERF13 and the E3 ubiquitin ligases MOS4-ASSOCIATED COMPLEX 3A (MAC3A) and MAC3B. As MAC3A and MAC3B gradually accumulate in the LR primordium, ERF13 levels gradually decrease. We demonstrate that MAC3A and MAC3B ubiquitinate ERF13, leading to its degradation and accelerating the transition of LR primordia from stages IV to V. Auxin enhances the MAC3A and MAC3B interaction with ERF13 by facilitating MPK14-mediated ERF13 phosphorylation. In summary, this study reveals the molecular mechanism by which auxin eliminates the inhibitory factor ERF13 through the MPK14-MAC3A and MAC3B signaling module, thus promoting LR emergence.
The chromatin remodeler ERCC6 and the histone chaperone NAP1 are involved in apurinic/apyrimidinic endonuclease-mediated DNA repair
Authors: Fan Tianyi , Shi Tianfang , Sui Ran , Wang Jingqi , Kang Huijia , Yu Yao , Zhu Yan
Published: February 2024

During base excision repair (BER), the apurinic or apyrimidinic (AP) site serves as an intermediate product following base excision. In plants, APE-redox protein (ARP) represents the major AP site of cleavage activity. Despite the well-established understanding that the nucleosomal structure acts as a barrier to various DNA-templated processes, the regulatory mechanisms underlying BER at the chromatin level remain elusive, especially in plants. In this study, we identified plant chromatin remodeler Excision Repair Cross-Complementing protein group 6 (ERCC6) and histone chaperone Nucleosome Assembly Protein 1 (NAP1) as interacting proteins with ARP. The catalytic ATPase domain of ERCC6 facilitates its interaction with both ARP and NAP1. Additionally, ERCC6 and NAP1 synergistically contribute to nucleosome sliding and exposure of hindered endonuclease cleavage sites. Loss-of-function mutations in Arabidopsis (Arabidopsis thaliana) ERCC6 or NAP1 resulted in arp-dependent plant hypersensitivity to 5-fluorouracil, a toxic agent inducing BER, and the accumulation of AP sites. Furthermore, similar protein interactions are also found in yeast cells, suggesting a conserved recruitment mechanism employed by the AP endonuclease to overcome chromatin barriers during BER progression.
Redirecting barley breeding for grass production through genome editing of Photoperiod-H1
Authors: Tezuka Daisuke , Cho Huikyong , Onodera Hitomi , Linghu Qianyan , Chijimatsu Takeshi , Hata Masahiro , Imai Ryozo
Published: February 2024

Genome editing enables precise modification to harness an elite grain-producing barley (Hordeum vulgare L.) cultivar for grass production.
Heat stress at the bicellular stage inhibits sperm cell development and transport into pollen tubes
Authors: Li Xingli , Bruckmann Astrid , Dresselhaus Thomas , Begcy Kevin
Published: February 2024

For successful double fertilization in flowering plants (angiosperms), pollen tubes deliver 2 nonmotile sperm cells toward female gametes (egg and central cell, respectively). Heatwaves, especially during the reproduction period, threaten male gametophyte (pollen) development, resulting in severe yield losses. Using maize (Zea mays) as a crop and grass model system, we found strong seed set reduction when moderate heat stress was applied for 2 d during the uni- and bicellular stages of pollen development. We show that heat stress accelerates pollen development and impairs pollen germination capabilities when applied at the unicellular stage. Heat stress at the bicellular stage impairs sperm cell development and transport into pollen tubes. To understand the course of the latter defects, we used marker lines and analyzed the transcriptomes of isolated sperm cells. Heat stress affected the expression of genes associated with transcription, RNA processing and translation, DNA replication, and the cell cycle. This included the genes encoding centromeric histone 3 (CENH3) and α-tubulin. Most genes that were misregulated encode proteins involved in the transition from metaphase to anaphase during pollen mitosis II. Heat stress also activated spindle assembly check point and meta- to anaphase transition genes in sperm cells. In summary, misregulation of the identified genes during heat stress at the bicellular stage results in sperm cell development and transport defects ultimately leading to sterility.
Forest demography and biomass accumulation rates are associated with transient mean tree size vs. density scaling relations
Authors: Yu Kailiang , Chen Han Y H , Gessler Arthur , Pugh Thomas A M , Searle Eric B , Allen Robert B , Pretzsch Hans , Ciais Philippe , Phillips Oliver L , Brienen Roel J W , Chu Chengjin , Xie Shubin , Ballantyne Ashley P
Published: February 2024

Linking individual and stand-level dynamics during forest development reveals a scaling relationship between mean tree size and tree density in forest stands, which integrates forest structure and function. However, the nature of this so-called scaling law and its variation across broad spatial scales remain unquantified, and its linkage with forest demographic processes and carbon dynamics remains elusive. In this study, we develop a theoretical framework and compile a broad-scale dataset of long-term sample forest stands (n = 1,433) from largely undisturbed forests to examine the association of temporal mean tree size vs. density scaling trajectories (slopes) with biomass accumulation rates and the sensitivity of scaling slopes to environmental and demographic drivers. The results empirically demonstrate a large variation of scaling slopes, ranging from −4 to −0.2, across forest stands in tropical, temperate, and boreal forest biomes. Steeper scaling slopes are associated with higher rates of biomass accumulation, resulting from a lower offset of forest growth by biomass loss from mortality. In North America, scaling slopes are positively correlated with forest stand age and rainfall seasonality, thus suggesting a higher rate of biomass accumulation in younger forests with lower rainfall seasonality. These results demonstrate the strong association of the transient mean tree size vs. density scaling trajectories with forest demography and biomass accumulation rates, thus highlighting the potential of leveraging forest structure properties to predict forest demography, carbon fluxes, and dynamics at broad spatial scales.
Identifying content-invariant neural signatures of perceptual vividness
Authors: Barnett Benjy , Andersen Lau M , Fleming Stephen M , Dijkstra Nadine
Published: February 2024

Some conscious experiences are more vivid than others. Although perceptual vividness is a key component of human consciousness, how variation in this magnitude property is registered by the human brain is unknown. A striking feature of neural codes for magnitude in other psychological domains, such as number or reward, is that the magnitude property is represented independently of its sensory features. To test whether perceptual vividness also covaries with neural codes that are invariant to sensory content, we reanalyzed existing magnetoencephalography and functional MRI data from two distinct studies which quantified perceptual vividness via subjective ratings of awareness and visibility. Using representational similarity and decoding analyses, we find evidence for content-invariant neural signatures of perceptual vividness distributed across visual, parietal, and frontal cortices. Our findings indicate that the neural correlates of subjective vividness may share similar properties to magnitude codes in other cognitive domains.
Network topology facilitates internet traffic control in autocracies
Authors: Keremoğlu Eda , Weidmann Nils B , Gamero-Garrido Alexander , Carisimo Esteban , Dainotti Alberto , Snoeren Alex C
Published: February 2024

Recent years have seen an increase in governmental interference in digital communication. Most research on this topic has focused on the application level, studying how content is manipulated or removed on websites, blogs, or social media. However, in order for governments to obtain and maintain control of digital data flows, they need to secure access to the network infrastructure at the level of Internet service providers. In this paper, we study how the network topology of the Internet varies across different political environments, distinguishing between control at the level of individual Internet users (access) and a higher level in the hierarchy of network carriers (transit). Using a novel method to estimate the structure of the Internet from network measurements, we show that in autocratic countries, state-owned (rather than privately owned) providers have a markedly higher degree of control over transit networks. We also show that state-owned Internet providers often provide Internet access abroad, with a clear focus on other autocratic countries. Together, these results suggest that in autocracies, the network infrastructure is organized in a way that is more susceptible to the monitoring and manipulation of Internet data flows by state-owned providers both domestically and abroad.
Geometry for low-inertia aerosol capture: Lessons from fog-basking beetles
Authors: Shahrokhian Aida , Chan Fan Kiat , Feng Jiansheng , Gazzola Mattia , King Hunter
Published: February 2024

Water in the form of windborne fog droplets supports life in many coastal arid regions, where natural selection has driven nontrivial physical adaptation toward its separation and collection. For two species of Namib desert beetle whose body geometry makes for a poor filter, subtle modifications in shape and texture have been previously associated with improved performance by facilitating water drainage from its collecting surface. However, little is known about the relevance of these modifications to the flow physics that underlies droplets’ impaction in the first place. We find, through coupled experiments and simulations, that such alterations can produce large relative gains in water collection by encouraging droplets to “slip” toward targets at the millimetric scale, and by disrupting boundary and lubrication layer effects at the microscopic scale. Our results offer a lesson in biological fog collection and design principles for controlling particle separation beyond the specific case of fog-basking beetles.
Hippocampal replay sequence governed by spontaneous brain-wide dynamics
Authors: Yang Yifan , Leopold David A , Duyn Jeff H , Liu Xiao
Published: February 2024

Neurons in the hippocampus exhibit spontaneous spiking activity during rest that appears to recapitulate previously experienced events. While this replay activity is frequently linked to memory consolidation and learning, the underlying mechanisms are not well understood. Recent large-scale neural recordings in mice have demonstrated that resting-state spontaneous activity is expressed as quasi-periodic cascades of spiking activity that pervade the forebrain, with each cascade engaging a high proportion of recorded neurons. Hippocampal ripples are known to be coordinated with cortical dynamics; however, less is known about the occurrence of replay activity relative to other brain-wide spontaneous events. Here we analyzed responses across the mouse brain to multiple viewings of natural movies, as well as subsequent patterns of neural activity during rest. We found that hippocampal neurons showed time-selectivity, with individual neurons responding consistently during particular moments of the movie. During rest, the population of time-selective hippocampal neurons showed both forward and time-reversed replay activity that matched the sequence observed in the movie. Importantly, these replay events were strongly time-locked to brain-wide spiking cascades, with forward and time-reversed replay activity associated with distinct cascade types. Thus, intrinsic hippocampal replay activity is temporally structured according to large-scale spontaneous physiology affecting areas throughout the forebrain. These findings shed light on the coordination between hippocampal and cortical circuits thought to be critical for memory consolidation.
Does prior military and/or law enforcement experience influence officer retention? A case study of the Charleston Police Department
Authors: O’Guinn Bradley J , Nader Elias , Goldberg Dannelle , Gibson Anthony
Published: February 2024

Police agencies across the country have relied on applicants with prior military and/or law enforcement experience to fill vacancies within their ranks for many years. Despite the strong push to recruit officers with these types of backgrounds, few scholars have tested whether prior military and/or law enforcement experience affects officer tenure within policing. In this study, we use hiring and employment data from the Charleston (SC) Police Department (CPD) to assess whether these highly sought after characteristics influence officer retention. Our analysis revealed that officers with prior military service were significantly more likely to leave the agency, and tended to do so about 20 months sooner, than officers who did not serve in the military. Prior law enforcement experience was unrelated to officer separation but had a significant impact on the length of time officers remained with the agency. These findings suggest that police agencies must reevaluate current approaches to recruitment and retention. However, more research is needed to determine why certain officers are more likely to resign and how agencies can better address the underlying needs of high-risk officers.
‘The Devil I Know’: Explaining Police Officer Intention to Transfer to Another Department
Authors: Scheer Charles , Downey Ragan A , Rossler Michael T
Published: February 2024

Police workforce management has persisted as a major organizational concern. Numerous compelling historical and organizational factors have contributed to smaller numbers of qualified or even interested applicants for police agencies to choose from, and disrupted police career longevity and even police services overall. To address their staffing needs, a growing number of organizations have increasingly shifted their focus to recruiting existing sworn and experienced officers from other departments. While there is increasing concern about officers transferring across organizations, little research has examined officer intentions to move laterally within the police profession, why officers choose to stay or leave, and the implications for staffing. Using theories of cross-organizational turnover from career psychology and a robust sample of more than 600 officers from 8 police agencies, this paper explores the impact of compensation, organizational values, perceptions of supervisors, and career advancement opportunities on officers’ intentions to pursue lateral transfers. Results indicated that compensation had limited influence on intentions to leave and that organizational characteristics exhibited much stronger relationships. Suggestions for keeping officers and assisting recruits in finding their potential organizational fit, impressions of the trajectory of this trend, and implications for theory and practice are offered.
Leaving so soon? An analysis of withdrawal patterns from academy training
Authors: Ryan Ann Marie , Chatterjee Deepshikha , von Seekamm Kurt , Magalona Joie , Liebler Juli , Alanis Jo M
Published: February 2024

Retention in policing may be currently viewed as more challenging than ever. One focal point of concern is turnover early in occupational socialization, such as while still in academy training. In this paper, we examine data on withdrawal from Academy training for one state police agency from 1934 to 2022. We address whether there are trends in Academy turnover over time and examine how/if retention is impacted by external factors such as the economic and historical/societal context. We conclude our work with a set of practical implications for considering how external events influence retention during training.
A content analysis of memoranda of understanding between Texas Public School Districts and law enforcement agencies
Authors: Valerio Lacette R , Vaughn Margaret Ashley , Ekren Elizabeth , Barnes Valerie R
Published: February 2024

Little is known collectively about the contents of school-based law enforcement (SBLE) memoranda of understanding (MOUs), despite emerging evidence they may improve program operation. Using Texas as an exploratory case study, where contracted programs are the most common form of SBLE in K-12 schools, this study conducts a content analysis of all known state SBLE MOUs (289), examining them using 40 provision-derived codes grouped in five topical categories (contract logistics, responsible parties, officer logistics, officer duties, and officer roles). Findings show that most MOUs cover a wide breadth of provisions, but could expand enumerating officer duties and programmatic qualities that literature associates with improved SBLE outcomes, as well as spreading awareness of provisions among stakeholders. The goals of the study are to understand how MOUs define SBLE programs in Texas, provide a foundation for further research examining SBLE program structures and outcomes, and make general recommendations for enhancing SBLE MOUs.
Job crafting: Does it have a role to play in improving well-being in policing?
Authors: Tehrani Noreen , Hesketh Ian
Published: February 2024

Working in policing can be psychologically challenging in an environment which requires officers and staff to be self-disciplined in adhering to procedural and legal frameworks. These role demands can lead to increased mental health problems. The ability to craft one’s job to personal and career needs and aspirations can reduce stress and burnout. This study aims to identify the level of job crafting and any benefit of job crafting in UK policing. Four thousand six hundred seventy-three police officers and staff completed an online survey as part of a regular psychological surveillance programme. The survey measured anxiety, depression, burnout, and traumatic stress together with a job crafting questionnaire. The results did not find any difference in mental health between officers and staff who used job crafting from those who did not. An analysis of comments provided insight into the role job crafting plays in policing. The evidence on the benefits of job crafting in terms of mental health is mixed. The study highlighted the need for job crafting to be seen in a broader context where an individual’s opportunity for flexibility and autonomy is balanced with their legal and professional obligations and organizational requirements.
Gender differences in Chinese policing: Supervisor support, wellbeing, and turnover intention
Authors: Ha Stephanie V , Sun Ivan Y , Wu Yuning , Chen Yunan
Published: February 2024

Many studies have been conducted over the past few decades to assess gender differences in police officers’ attitudes towards critical aspects of policing. Nonetheless, this line of inquiry has focused predominately on police officers in Western democracies, with limited attention to gender differences in societies where cultural traditions and gender inequalities remain influential in shaping police officers’ occupational attitudes. Using survey data collected from over 1,900 police officers in a northern Chinese province, this study assesses whether female and male officers differ in their attitudes towards supervisor treatment, psychological and physical conditions, and intention to leave the profession. We found that female police officers displayed occupational attitudes that distinguish them from their male counterparts. Contrary to Western evidence, Chinese female officers expressed lower intentions to leave the police profession than male officers. Female officers also reported better wellbeing with lower stress and health concerns than their male counterparts. We also found that supervisors’ procedurally fair treatment and support of technological advancement are associated with lower turnover intention. Implications for policy and future research are discussed.