bluuebunny/crossref_metadata_2025 huggingface dataset:
Selecting sorting centres to avoid long distance transport of weaned beef calves
Authors: Morel-Journel T. , Vergu E. , Mercier J.-B. , Bareille N. , Ezanno P.
Published: January 2021

The transport of weaned calves from cow–calf producers to fatteners is a general concern for the young bull industry due to its documented negative impact on the welfare, health and performance of the animals. These transfers are often managed by intermediaries who transport weaned calves to sorting centres, where they are grouped into batches before being sent to fattening units. In this study, we present an algorithm to limiting these transfer distances by appropriately selecting the sorting centre through which they must go. We tested the effectiveness of this algorithm on historical data from a French beef producer organization managing 136,892 transfers using 13 sorting centres. The results show a decrease in the transfer distances compared to the historical record, especially for the calves travelling over long distances (− 76 km, i.e. 18% on average for the 33% longest transfers). Moreover, the distribution of calves between the sorting centres proposed by the algorithm reveals differences in their efficiency in minimizing transfer distances. In addition to its usefulness as a management tool for the daily transport of cattle, this algorithm provides prospects for improving the management of the sorting centres themselves.
Inferring mechanisms of response prioritization on social media under information overload
Authors: Gunaratne Chathika , Rand William , Garibay Ivan
Published: January 2021

Human decision-making is subject to the biological limits of cognition. The fluidity of information propagation over online social media often leads users to experience information overload. This in turn affects which information received by users are processed and gain a response to, imposing constraints on volumes of, and participation in, information cascades. In this study, we investigate properties contributing to the visibility of online social media notifications by highly active users experiencing information overload via cross-platform social influence. We analyze simulations of a coupled agent-based model of information overload and the multi-action cascade model of conversation with evolutionary model discovery. Evolutionary model discovery automates mechanistic inference on agent-based models by enabling random forest importance analysis on genetically programmed agent-based model rules. The mechanisms of information overload have shown to contribute to a multitude of global properties of online information cascades. We investigate nine characteristics of online messages that may contribute to the prioritization of messages for response. Our results indicate that recency had the largest contribution to message visibility, with individuals prioritizing more recent notifications. Global popularity of the conversation originator had the second highest contribution, and reduced message visibility. Messages that presented opportunity for novel user interaction, yet high reciprocity showed to have relatively moderate contribution to message visibility. Finally, insights from the evolutionary model discovery results helped inform response prioritization rules, which improved the robustness and accuracy of the model of information overload.
Acquisition of concrete and abstract words is modulated by tDCS of Wernicke’s area
Authors: Kurmakaeva Diana , Blagovechtchenski Evgeny , Gnedykh Daria , Mkrtychian Nadezhda , Kostromina Svetlana , Shtyrov Yury
Published: January 2021

Previous behavioural and neuroimaging research suggested distinct cortical systems involved in processing abstract and concrete semantics; however, there is a dearth of causal evidence to support this. To address this, we applied anodal, cathodal, or sham (placebo) tDCS over Wernicke’s area before a session of contextual learning of novel concrete and abstract words (n = 10 each), presented five times in short stories. Learning effects were assessed at lexical and semantic levels immediately after the training and, to attest any consolidation effects of overnight sleep, on the next day. We observed successful learning of all items immediately after the session, with decreased performance in Day 2 assessment. Importantly, the results differed between stimulation conditions and tasks. Whereas the accuracy of semantic judgement for abstract words was significantly lower in the sham and anodal groups on Day 2 vs. Day 1, no significant performance drop was observed in the cathodal group. Similarly, the cathodal group showed no significant overnight performance reduction in the free recall task for either of the stimuli, unlike the other two groups. Furthermore, between-group analysis showed an overall better performance of both tDCS groups over the sham group, particularly expressed for abstract semantics and cathodal stimulation. In sum, the results suggest overlapping but diverging brain mechanisms for concrete and abstract semantics and indicate a larger degree of involvement of core language areas in storing abstract knowledge. Furthermore, they demonstrate a possiblity to improve learning outcomes using neuromodulatory techniques.
The effects of meldonium on the acute ischemia/reperfusion liver injury in rats
Authors: Đurašević Siniša , Stojković Maja , Sopta Jelena , Pavlović Slađan , Borković-Mitić Slavica , Ivanović Anđelija , Jasnić Nebojša , Tosti Tomislav , Đurović Saša , Đorđević Jelena , Todorović Zoran
Published: January 2021

Acute ischemia/reperfusion (I/R) liver injury is a clinical condition challenging to treat. Meldonium is an anti-ischemic agent that shifts energy production from fatty acid oxidation to less oxygen-consuming glycolysis. Thus, we investigated the effects of a 4-week meldonium pre-treatment (300 mg/kg b.m./day) on the acute I/R liver injury in Wistar strain male rats. Our results showed that meldonium ameliorates I/R-induced liver inflammation and injury, as confirmed by liver histology, and by attenuation of serum alanine- and aspartate aminotransferase activity, serum and liver high mobility group box 1 protein expression, and liver expression of Bax/Bcl2, haptoglobin, and the phosphorylated nuclear factor kappa-light-chain-enhancer of activated B cells. Through the increased hepatic activation of the nuclear factor erythroid 2-related factor 2, meldonium improves the antioxidative defence in the liver of animals subjected to I/R, as proved by an increase in serum and liver ascorbic/dehydroascorbic acid ratio, hepatic haem oxygenase 1 expression, glutathione and free thiol groups content, and hepatic copper-zinc superoxide dismutase, manganese superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity. Based on our results, it can be concluded that meldonium represent a protective agent against I/R-induced liver injury, with a clinical significance in surgical procedures.
Climate change impact on the potential geographical distribution of two invading Xylosandrus ambrosia beetles
Authors: Urvois T. , Auger-Rozenberg M. A. , Roques A. , Rossi J. P. , Kerdelhue C.
Published: January 2021

Xylosandrus compactus and X. crassiusculus are two polyphagous ambrosia beetles originating from Asia and invasive in circumtropical regions worldwide. Both species were recently reported in Italy and further invaded several other European countries in the following years. We used the MaxEnt algorithm to estimate the suitable areas worldwide for both species under the current climate. We also made future projections for years 2050 and 2070 using 11 different General Circulation Models, for 4 Representative Concentration Pathways (2.6, 4.5, 6.0 and 8.5). Our analyses showed that X. compactus has not been reported in all potentially suitable areas yet. Its current distribution in Europe is localised, whereas our results predicted that most of the periphery of the Mediterranean Sea and most of the Atlantic coast of France could be suitable. Outside Europe, our results also predicted Central America, all islands in Southeast Asia and some Oceanian coasts as suitable. Even though our results when modelling its potential distribution under future climates were more variable, the models predicted an increase in suitability poleward and more uncertainty in the circumtropical regions. For X. crassiusculus, the same method only yielded poor results, and the models thus could not be used for predictions. We discuss here these results and propose advice about risk prevention and invasion management of both species.
Computational exploration of copper catalyzed vinylogous aerobic oxidation of unsaturated compounds
Authors: Wang Ting , Zhou Yu , Xu Yao , Cheng Gui-Juan
Published: January 2021

Selective oxidation is one of the most important and challenging transformations in both academic research and chemical industry. Recently, a highly selective and efficient way to synthesize biologically activeγ-hydroxy-α,β-unsaturated molecules from Cu-catalyzed vinylogous aerobic oxidation ofα,β- andβ,γ-unsaturated compounds has been developed. However, the detailed reaction mechanism remains elusive. Herein, we report a density functional theory study on this Cu-catalyzed vinylogous aerobic oxidation ofγ,γ-disubstitutedα,β- andβ,γ-unsaturated isomers. Our computational study unveils detailed mechanism for each elementary step, i.e. deprotonation, O2activation, and reduction. Besides, the origin of regioselectivity, divergent reactivities of substrates as well as reducing agents, and the byproduct generation have also been investigated. Notably, the copper catalyst retains the + 2 oxidation state through the whole catalytic cycle and plays essential roles in multiple steps. These findings would provide hints on mechanistic studies and future development of transition metal-catalyzed aerobic oxidation reactions.
Thermal prediction of turbulent forced convection of nanofluid using computational fluid dynamics coupled genetic algorithm with fuzzy interface system
Authors: Babanezhad Meisam , Behroyan Iman , Nakhjiri Ali Taghvaie , Rezakazemi Mashallah , Marjani Azam , Shirazian Saeed
Published: January 2021

Computational fluid dynamics (CFD) simulating is a useful methodology for reduction of experiments and their associated costs. Although the CFD could predict all hydro-thermal parameters of fluid flows, the connections between such parameters with each other are impossible using this approach. Machine learning by the artificial intelligence (AI) algorithm has already shown the ability to intelligently record engineering data. However, there are no studies available to deeply investigate the implicit connections between the variables resulted from the CFD. The present investigation tries to conduct cooperation between the mechanistic CFD and the artificial algorithm. The genetic algorithm is combined with the fuzzy interface system (GAFIS). Turbulent forced convection of Al2O3/water nanofluid in a heated tube is simulated for inlet temperatures (i.e., 305, 310, 315, and 320 K). GAFIS learns nodes coordinates of the fluid, the inlet temperatures, and turbulent kinetic energy (TKE) as inputs. The fluid temperature is learned as output. The number of inputs, population size, and the component are checked for the best intelligence. Finally, at the best intelligence, a formula is developed to make a relationship between the output (i.e. nanofluid temperatures) and inputs (the coordinates of the nodes of the nanofluid, inlet temperature, and TKE). The results revealed that the GAFIS intelligence reaches the highest level when the input number, the population size, and the exponent are 5, 30, and 3, respectively. Adding the turbulent kinetic energy as the fifth input, the regression value increases from 0.95 to 0.98. This means that by considering the turbulent kinetic energy the GAFIS reaches a higher level of intelligence by distinguishing the more difference between the learned data. The CFD and GAFIS predicted the same values of the nanofluid temperature.
Mutations of folC cause increased susceptibility to sulfamethoxazole in Mycobacterium tuberculosis
Authors: Wang Ruiqi , Li Kun , Yu Jifang , Deng Jiaoyu , Chen Yaokai
Published: January 2021

Previous studies showed that mutation of folC caused decreased expression of the dihydropteroate synthase encoding gene folP2 in Mycobacterium tuberculosis (M. tuberculosis). We speculated that mutation of folC in M. tuberculosis might affect the susceptibility to sulfamethoxazole (SMX). To prove this, 53 clinical isolates with folC mutations were selected and two folC mutants (I43A, I43T) were constructed based on M. tuberculosis H37Ra. The results showed that 42 of the 53 clinical isolates (79.2%) and the two lab-constructed folC mutants were more sensitive to SMX. To probe the mechanism by which folC mutations make M. tuberculosis more sensitive to SMX, folP2 was deleted in H37Ra, and expression levels of folP2 were compared between H37Ra and the two folC mutants. Although deletion of folP2 resulted in increased susceptibility to SMX, no difference in folP2 expression was observed. Furthermore, production levels of para-aminobenzoic acid (pABA) were compared between the folC mutants and the wild-type strain, and results showed that folC mutation resulted in decreased production of pABA. Taken together, we show that folC mutation leads to decreased production of pABA in M. tuberculosis and thus affects its susceptibility to SMX, which broadens our understanding of mechanisms of susceptibilities to antifolates in this bacterium.
Group and individual level variations between symmetric and asymmetric DLPFC montages for tDCS over large scale brain network nodes
Authors: Soleimani Ghazaleh , Saviz Mehrdad , Bikson Marom , Towhidkhah Farzad , Kuplicki Rayus , Paulus Martin P. , Ekhtiari Hamed
Published: January 2021

Two challenges to optimizing transcranial direct current stimulation (tDCS) are selecting between, often similar, electrode montages and accounting for inter-individual differences in response. These two factors are related by how tDCS montage determines current flow through the brain considered across or within individuals. MRI-based computational head models (CHMs) predict how brain anatomy determines electric field (EF) patterns for a given tDCS montage. Because conventional tDCS produces diffuse brain current flow, stimulation outcomes may be understood as modulation of global networks. Therefore, we developed a network-led, rather than region-led, approach. We specifically considered two common “frontal” tDCS montages that nominally target the dorsolateral prefrontal cortex; asymmetric “unilateral” (anode/cathode: F4/Fp1) and symmetric “bilateral” (F4/F3) electrode montages. CHMs of 66 participants were constructed. We showed that cathode location significantly affects EFs in the limbic network. Furthermore, using a finer parcellation of large-scale networks, we found significant differences in some of the main nodes within a network, even if there is no difference at the network level. This study generally demonstrates a methodology for considering the components of large-scale networks in CHMs instead of targeting a single region and specifically provides insight into how symmetric vs asymmetric frontal tDCS may differentially modulate networks across a population.
Estimating the health burden of aflatoxin attributable stunting among children in low income countries of Africa
Authors: Rasheed Hifza , Xu Ya , Kimanya Martin E. , Pan Xiaoxi , Li Zhihua , Zou Xiaobo , Shirima Candida P. , Holmes Melvin , Routledge Michael N. , Gong Yun Yun
Published: January 2021

Numerous population-based studies have documented high prevalence of aflatoxin associated childhood stunting in low income countries. We provide an estimate of the disease burden of aflatoxin related stunting using data from the four African countries. For this empirical analysis, we obtained blood aflatoxin albumin adduct biomarker based exposure data as measured using ELISA technique and anthropometric measurement data from surveys done over a 12-year period from 2001 to 2012 in four low income countries in Africa. We used these data to calculate population attributable risk (PAR), life time disease burden for children under five by comparing two groups of stunted children using both prevalence and incidence-based approaches. We combined prevalence estimates with a disability weight, measuring childhood stunting and co-occurrence of stunting-underweight to produce years lived with disability. Using a previously reported mortality, years of life lost were estimated. We used probabilistic analysis to model these associations to estimate the disability-adjusted life-years (DALYs), and compared these with those given by the Institute for Health Metrics and Evaluation’s Global Burden of Disease (GBD) 2016 study. The PAR increased from 3 to 36% for aflatoxin-related stunting and 14–50% for co-occurrence of stunting and underweight. Using prevalence-based approach, children with aflatoxin related stunting resulted in 48,965.20 (95% uncertainty interval (UI): 45,868.75–52,207.53) DALYs per 100,000 individuals. Children with co-occurrence of stunting and underweight due to exposure to aflatoxin resulted in 40,703.41 (95% UI: 38,041.57–43,517.89) DALYs per 100,000 individuals. Uncertainty analysis revealed that reducing aflatoxin exposure in high exposure areas upto non-detectable levels could save the stunting DALYs up to 50%. The burden of childhood all causes stunting is greater in countries with higher aflatoxin exposure such as Benin. In high exposure areas, these results might help guide research protocols and prioritisation efforts and focus aflatoxin exposure reduction. HEFCE Global Challenge Research Fund Aflatoxin project.
Design and testing of a soft parallel robot based on pneumatic artificial muscles for wrist rehabilitation
Authors: Wang Yaxi , Xu Qingsong
Published: January 2021

Wrist rehabilitation is needed to help post-stroke and post-surgery patients recover from wrist fracture or injury. Traditional rehabilitation training is conducted by a therapist in a hospital, which hinders timely treatment due to the corresponding time and space constraints. This paper presents the design and implementation of a soft parallel robot for automated wrist rehabilitation. The presented wrist rehabilitation robot integrates the advantages of both soft robot and parallel robot structures. Unlike traditional rigid-body based rehabilitation robots, this soft parallel robot exhibits a compact structure, which is highly secure, adaptable, and flexible and thus a low-cost solution for personalized treatment. The proposed soft wrist-rehabilitation robot is driven by six evenly distributed linear actuators using pneumatic artificial muscles and one central linear electric motor. The introduced parallel-kinematic mechanism design enables the enhancement of the output stiffness of the soft robot for practical use. An electromyography sensor is adopted to provide feedback signals for evaluating the rehabilitation training process. A kinematic model of the designed robot is derived, and a prototype is fabricated for experimental testing. The results demonstrate that the developed soft rehabilitation robot can assist the wrist to realize all the required training motions, including abduction-adduction, flexion-extension, and supination-pronation. The compact and lightweight structure of this novel robot makes it convenient to use, and suitable rehabilitation training modes can be chosen for tailored rehabilitation at home or in a hospital.
Fetal bovine serum albumin inhibits antimicrobial peptide activity and binds drug only in complex with α1-antitrypsin
Authors: Tang Wen-Hung , Wang Chiu-Feng , Liao You-Di
Published: January 2021

Several antimicrobial peptides (AMPs) have been developed for the treatment of infections caused by antibiotic-resistant microbes, but their applications are primarily limited to topical infections because in circulation they are bound and inhibited by serum proteins. Here we have found that some AMPs, such as TP4 from fish tilapia, and drugs, such as antipyretic ibuprofen, were bound by bovine serum albumin only in complex with α1-antitrypsin which is linked by disulfide bond. They existed in dimeric complex (2 albumin -2 α1-antitrypsin) in the bovine serum only at fetal stage, but not after birth. The hydrophobic residues of TP4 were responsible for its binding to the complex. Since bovine serum is a major supplement in most cell culture media, therefore the existence and depletion of active albumin/α1-antitrypsin complex are very important for the assay and production of biomolecules.
Annual phytoplankton dynamics in coastal waters from Fildes Bay, Western Antarctic Peninsula
Authors: Trefault Nicole , De la Iglesia Rodrigo , Moreno-Pino Mario , Lopes dos Santos Adriana , Gérikas Ribeiro Catherine , Parada-Pozo Génesis , Cristi Antonia , Marie Dominique , Vaulot Daniel
Published: January 2021

Year-round reports of phytoplankton dynamics in the West Antarctic Peninsula are rare and mainly limited to microscopy and/or pigment-based studies. We analyzed the phytoplankton community from coastal waters of Fildes Bay in the West Antarctic Peninsula between January 2014 and 2015 using metabarcoding of the nuclear and plastidial 18/16S rRNA gene from both size-fractionated and flow cytometry sorted samples. Overall 14 classes of photosynthetic eukaryotes were present in our samples with the following dominating: Bacillariophyta (diatoms), Pelagophyceae and Dictyochophyceae for division Ochrophyta, Mamiellophyceae and Pyramimonadophyceae for division Chlorophyta, Haptophyta and Cryptophyta. Each metabarcoding approach yielded a different image of the phytoplankton community with for example Prymnesiophyceae more prevalent in plastidial metabarcodes and Mamiellophyceae in nuclear ones. Diatoms were dominant in the larger size fractions and during summer, while Prymnesiophyceae and Cryptophyceae were dominant in colder seasons. Pelagophyceae were particularly abundant towards the end of autumn (May). In addition of Micromonas polaris and Micromonas sp. clade B3, both previously reported in Arctic waters, we detected a new Micromonas 18S rRNA sequence signature, close to, but clearly distinct from M. polaris, which potentially represents a new clade specific of the Antarctic. These results highlight the need for complementary strategies as well as the importance of year-round monitoring for a comprehensive description of phytoplankton communities in Antarctic coastal waters.
Memory-specific correlated neuronal activity in higher-order auditory regions of a parrot
Authors: Satoh Ryohei , Eda-Fujiwara Hiroko , Watanabe Aiko , Okamoto Yasuharu , Miyamoto Takenori , Zandbergen Matthijs A. , Bolhuis Johan J.
Published: January 2021

Male budgerigars (Melopsittacus undulatus) are open-ended learners that can learn to produce new vocalisations as adults. We investigated neuronal activation in male budgerigars using the expression of the protein products of the immediate early geneszenkandc-fosin response to exposure to conspecific contact calls (CCs: that of the mate or an unfamiliar female) in three subregions (CMM, dNCM and vNCM) of the caudomedial pallium, a higher order auditory region. Significant positive correlations of Zenk expression were found between these subregions after exposure to mate CCs. In contrast, exposure to CCs of unfamiliar females produced no such correlations. These results suggest the presence of a CC-specific association among the subregions involved in auditory memory. The caudomedial pallium of the male budgerigar may have functional subdivisions that cooperate in the neuronal representation of auditory memory.
Reconstructing Late Pleistocene paleoclimate at the scale of human behavior: an example from the Neandertal occupation of La Ferrassie (France)
Authors: Pederzani Sarah , Aldeias Vera , Dibble Harold L. , Goldberg Paul , Hublin Jean-Jacques , Madelaine Stéphane , McPherron Shannon P. , Sandgathe Dennis , Steele Teresa E. , Turq Alain , Britton Kate
Published: January 2021

Exploring the role of changing climates in human evolution is currently impeded by a scarcity of climatic information at the same temporal scale as the human behaviors documented in archaeological sites. This is mainly caused by high uncertainties in the chronometric dates used to correlate long-term climatic records with archaeological deposits. One solution is to generate climatic data directly from archaeological materials representing human behavior. Here we use oxygen isotope measurements of Bos/Bison tooth enamel to reconstruct summer and winter temperatures in the Late Pleistocene when Neandertals were using the site of La Ferrassie. Our results indicate that, despite the generally cold conditions of the broader period and despite direct evidence for cold features in certain sediments at the site, Neandertals used the site predominantly when climatic conditions were mild, similar to conditions in modern day France. We suggest that due to millennial scale climate variability, the periods of human activity and their climatic characteristics may not be representative of average conditions inferred from chronological correlations with long-term climatic records. These results highlight the importance of using direct routes, such as the high-resolution archives in tooth enamel from anthropogenically accumulated faunal assemblages, to establish climatic conditions at a human scale.
Post-gel polymerisation shrinkage profiling of polymer biomaterials using a chirped fibre Bragg grating
Authors: Rajan Ginu , Wong Alex , Farrar Paul , Prusty Gangadhara B.
Published: January 2021

A strain profile measurement technique using a chirped fibre Bragg grating (CFBG) sensor by implementing an integration of differences (IOD) method is reported in this paper. Using the IOD method the spatial distribution of strain along the length of the CFBG is extracted from its power reflectance spectra. As a proof of concept demonstration, the developed technique is applied to measure the polymerisation shrinkage strain profile of a photo-cured polymer dental composite which exhibits a non-uniform strain distribution attributed to the curing lamp characteristics. The result from the CFBG technique is compared with that of an FBG array embedded in the dental composite and is correlated with the degree of conversion of the material which also depends on the curing lamp intensity distribution. This technology will have significant impact and applications in a range of medical, materials and engineering areas where strain or temperature gradient profile measurement is required in smaller scales.
Myocyte-specific enhancer factor 2c triggers transdifferentiation of adipose tissue-derived stromal cells into spontaneously beating cardiomyocyte-like cells
Authors: Takashima Shinichiro , Usui Soichiro , Inoue Oto , Goten Chiaki , Yamaguchi Kosei , Takeda Yusuke , Cui Shihe , Sakai Yoshio , Hayashi Kenshi , Sakata Kenji , Kawashiri Masa-aki , Takamura Masayuki
Published: January 2021

Cardiomyocyte regeneration is limited in adults. The adipose tissue-derived stromal vascular fraction (Ad-SVF) contains pluripotent stem cells that rarely transdifferentiate into spontaneously beating cardiomyocyte-like cells (beating CMs). However, the characteristics of beating CMs and the factors that regulate the differentiation of Ad-SVF toward the cardiac lineage are unknown. We developed a simple culture protocol under which the adult murine inguinal Ad-SVF reproducibly transdifferentiates into beating CMs without induction. The beating CMs showed the striated ventricular phenotype of cardiomyocytes and synchronised oscillation of the intracellular calcium concentration among cells on day 28 of Ad-SVF primary culture. We also identified beating CM-fated progenitors (CFPs) and performed single-cell transcriptome analysis of these CFPs. Among 491 transcription factors that were differentially expressed (≥ 1.75-fold) in CFPs and the beating CMs, myocyte-specific enhancer 2c (Mef2c) was key. Transduction of Ad-SVF cells with Mef2c using a lentiviral vector yielded CFPs and beating CMs with ~ tenfold higher cardiac troponin T expression, which was abolished by silencing of Mef2c. Thus, we identified the master gene required for transdifferentiation of Ad-SVF into beating CMs. These findings will facilitate the development of novel cardiac regeneration therapies based on gene-modified, cardiac lineage-directed Ad-SVF cells.
Vasoactive intestinal polypeptide plasma levels associated with affective symptoms and brain structure and function in healthy females
Authors: Simon Rozalyn A. , Barazanji Nawroz , Jones Michael P. , Bednarska Olga , Icenhour Adriane , Engström Maria , Hamilton J. Paul , Keita Åsa V. , Walter Susanna
Published: January 2021

Vasoactive intestinal polypeptide (VIP) is a neuroendocrine peptide distributed throughout the human body, including the CNS, where it is particularly abundant in brain regions associated with anxiety and depression. Based on earlier studies indicating that peripheral VIP may cross through the blood–brain barrier, we hypothesized plasma VIP levels to be associated with symptoms of anxiety and depression, as well as brain volume and resting-state functional connectivity in the amygdala, hippocampus, parahippocampus, and orbitofrontal cortex. Plasma VIP concentrations and anxiety/depression symptoms were measured in 37 healthy females. Functional and structural magnetic resonance imaging were used to evaluate functional connectivity and brain volume respectively, and their associations with VIP concentrations within brain regions associated with anxiety and depression. Negative correlations were found between VIP levels and symptoms of anxiety (r = − 0.44,p = 0.002) and depression (r = − 0.50,p = 0.001). Functional connectivity demonstrated significant VIP-dependent positive associations between the amygdala seed region with both the right parahippocampus (t(33) = 3.1, pFDR = 0.02) and right lateral orbitofrontal cortex (OFC;t(33) = 2.9, pFDR = 0.02). Moreover, VIP concentrations were significantly, positively correlated with brain volume in the left amygdala (r = 0.28,p = 0.007) and left lateral OFC (r = 0.29,p = 0.004). The present findings highlight a potential role for VIP in the neurobiology of affective symptoms.
The role of Toll-like receptor 9 in a murine model of Cryptococcus gattii infection
Authors: da Silva-Junior Elias Barbosa , Firmino-Cruz Luan , Guimarães-de-Oliveira Joyce Cristina , De-Medeiros Juliana Valente Rodrigues , de Oliveira Nascimento Danielle , Freire-de-Lima Matheus , de Brito-Gitirana Lycia , Morrot Alexandre , Previato Jose Osvaldo , Mendonça-Previato Lucia , Decote-Ricardo Debora , de Matos Guedes Herbert Leonel , Freire-de-Lima Celio Geraldo
Published: January 2021

Toll-like receptor 9 (TLR9) is crucial to the host immune response against fungi, such as Candida albicans, Aspergillus fumigatus and Cryptococcus neoformans, but its importance in Cryptococcus gattii infection is unknown. Our study aimed to understand the role of TLR9 during the course of experimental C. gattii infection in vivo, considering that the cryptococcal DNA interaction with the receptor could contribute to host immunity even in an extremely susceptible model. We inoculated C57BL/6 (WT) and TLR9 knock-out (TLR9−/−) mice intratracheally with 104C. gattii yeast cells. TLR9−/− mice had a higher mortality rate compared to WT mice and more yeast cells that had abnormal size, known as titan cells, in the lungs. TLR9−/− mice also had a greater number of CFUs in the spleen and brain than WT mice, in addition to having lower levels of IFN-γ and IL-17 in the lung. With these markers of aggressive cryptococcosis, we can state that TLR9−/− mice are more susceptible to C. gattii, probably due to a mechanism associated with the decrease of a Th1 and Th17-type immune response that promotes the formation of titan cells in the lungs. Therefore, our results indicate the participation of TLR9 in murine resistance to C. gattii infection.
Multifocal acceptance score to evaluate vision: MAS-2EV
Authors: Barcala Xoana , Vinas Maria , Romero Mercedes , Gambra Enrique , Mendez-Gonzalez Juan Luis , Marcos Susana , Dorronsoro Carlos
Published: January 2021

We present a new metric (Multifocal Acceptance Score, MAS-2EV) to evaluate vision with presbyopic corrections. The MAS-2EV is based on a set of images representing natural visual scenes at day and night conditions projected in far and near displays, and a near stereo target. Subjects view and score the images through different binocular corrections (monofocal corrections at far; bifocal corrections; monovision and modified monovision) administered with soft contact lenses (in cyclopleged young subjects) or with a binocular simultaneous vision simulator (in presbyopic and cyclopleged young subjects). MAS-2EV scores are visually represented in the form of polygons, and quantified using different metrics: overall visual quality, visual degradation at far, visual benefit at near, near stereo benefit, visual imbalance near-far, overall visual imbalance and a combined overall performance metric. We have found that the MAS-2EV has sufficient repeatability and sensitivity to allow differentiation across corrections with only two repetitions, and the duration of the psychophysical task (3 min for subject/condition/correction) makes it useable in the clinic. We found that in most subjects binocular bifocal corrections produce the lowest visual imbalance, and the highest near stereo benefit. 46.67% of the subjects ranked binocular bifocal corrections first, and 46.67% of the subjects ranked monovision first. MAS-2EV, particularly in combination with visual simulators, can be applied to select prospective presbyopic corrections in patients prior to contact lens fitting or intraocular lens implantation.
Predicting bacteriophage hosts based on sequences of annotated receptor-binding proteins
Authors: Boeckaerts Dimitri , Stock Michiel , Criel Bjorn , Gerstmans Hans , De Baets Bernard , Briers Yves
Published: January 2021

Nowadays, bacteriophages are increasingly considered as an alternative treatment for a variety of bacterial infections in cases where classical antibiotics have become ineffective. However, characterizing the host specificity of phages remains a labor- and time-intensive process. In order to alleviate this burden, we have developed a new machine-learning-based pipeline to predict bacteriophage hosts based on annotated receptor-binding protein (RBP) sequence data. We focus on predicting bacterial hosts from the ESKAPE group, Escherichia coli, Salmonella enterica and Clostridium difficile. We compare the performance of our predictive model with that of the widely used Basic Local Alignment Search Tool (BLAST). Our best-performing predictive model reaches Precision-Recall Area Under the Curve (PR-AUC) scores between 73.6 and 93.8% for different levels of sequence similarity in the collected data. Our model reaches a performance comparable to that of BLASTp when sequence similarity in the data is high and starts outperforming BLASTp when sequence similarity drops below 75%. Therefore, our machine learning methods can be especially useful in settings in which sequence similarity to other known sequences is low. Predicting the hosts of novel metagenomic RBP sequences could extend our toolbox to tune the host spectrum of phages or phage tail-like bacteriocins by swapping RBPs.
Integrating epidemiological and genetic data with different sampling intensities into a dynamic model of respiratory syncytial virus transmission
Authors: Kombe Ivy K. , Agoti Charles N. , Munywoki Patrick K. , Baguelin Marc , Nokes D. James , Medley Graham F.
Published: January 2021

Respiratory syncytial virus (RSV) is responsible for a significant burden of severe acute lower respiratory tract illness in children under 5 years old; particularly infants. Prior to rolling out any vaccination program, identification of the source of infant infections could further guide vaccination strategies. We extended a dynamic model calibrated at the individual host level initially fit to social-temporal data on shedding patterns to include whole genome sequencing data available at a lower sampling intensity. The study population was 493 individuals (55 aged < 1 year) distributed across 47 households, observed through one RSV season in coastal Kenya. We found that 58/97 (60%) of RSV-A and 65/125 (52%) of RSV-B cases arose from infection probably occurring within the household. Nineteen (45%) infant infections appeared to be the result of infection by other household members, of which 13 (68%) were a result of transmission from a household co-occupant aged between 2 and 13 years. The applicability of genomic data in studies of transmission dynamics is highly context specific; influenced by the question, data collection protocols and pathogen under investigation. The results further highlight the importance of pre-school and school-aged children in RSV transmission, particularly the role they play in directly infecting the household infant. These age groups are a potential RSV vaccination target group.
Symptoms of anxiety/depression is associated with more aggressive inflammatory bowel disease
Authors: Gao Xin , Tang Yu , Lei Na , Luo Ying , Chen Pingrun , Liang Chang , Duan Shihao , Zhang Yan
Published: January 2021

Studies have demonstrated that inflammatory bowel disease (IBD) patients are at an increased risk of developing anxiety and/or depression. IBD patients with depression/anxiety have higher rates of hospitalization and increased disease severity than those without. So far, there is a paucity of data concerning the impact of anxiety/depression on Chinese IBD patients. The aim of this study was to find out the prevalence of symptoms of anxiety/depression in Chinese IBD population and its impact on IBD-related features. This is a cross-sectional study from the southwest China IBD referral center. Eligible participants were divided into those with symptoms of anxiety/depression and those without based on the Hospital Anxiety and Depression Scale (HADS). Demographic data and disease duration, IBD-related surgery, tobacco use, extra-intestinal manifestations, disease activity scores, endoscopic evaluation, laboratory data and current medication use were compared between two groups. A total of 341 IBD patients [221 Crohn’s disease (CD) and 120 ulcerative colitis (UC)] were included. The prevalence of symptoms of anxiety/depression in IBD was 33.1%. CD patients with symptoms of anxiety/depression tended to have higher scores of simple endoscopic scores for Crohn’s disease (SES-CD) (p = 0.0005). UC patients with symptoms of anxiety/depression had a significantly higher Mayo score (p = 0.0017) and ulcerative colitis endoscopic index of severity (UCEIS) (p < 0.0001) than their non-anxiety/depression counterparts. CD-related surgery (p = 0.012) and Crohn's disease activity index (CDAI) (p < 0.0001) were identified as independent risk factors for symptoms of anxiety/depression in CD, while corticosteroid use (p = 0.036) as an independent risk factor for symptoms of anxiety/depression in UC. This study helps our understanding of the prevalence of symptoms of anxiety/depression in IBD patients and its impact on IBD course and reminds us to pay more attention on IBD management with anxiety/depression.
Brain reactivity using fMRI to insomnia stimuli in insomnia patients with discrepancy between subjective and objective sleep
Authors: Kim Young-Bo , Kim Nambeom , Lee Jae Jun , Cho Seo-Eun , Na Kyoung-Sae , Kang Seung-Gul
Published: January 2021

Subjective–objective discrepancy of sleep (SODS) might be related to the distorted perception of sleep deficit and hypersensitivity to insomnia-related stimuli. We investigated differences in brain activation to insomnia-related stimuli among insomnia patients with SODS (SODS group), insomnia patients without SODS (NOSODS group), and healthy controls (HC). Participants were evaluated for subjective and objective sleep using sleep diary and polysomnography. Functional magnetic resonance imaging was conducted during the presentation of insomnia-related (Ins), general anxiety-inducing (Gen), and neutral (Neu) stimuli. Brain reactivity to the contrast of Ins vs. Neu and Gen vs. Neu was compared among the SODS (n = 13), NOSODS (n = 15), and HC (n = 16) groups. In the SODS group compared to other groups, brain areas including the left fusiform, bilateral precuneus, right superior frontal gyrus, genu of corpus callosum, and bilateral anterior corona radiata showed significantly increased blood oxygen level dependent (BOLD) signal in the contrast of Ins vs. Neu. There was no brain region with significantly increased BOLD signal in the Gen vs. Neu contrast in the group comparisons. Increased brain activity to insomnia-related stimuli in several brain regions of the SODS group is likely due to these individuals being more sensitive to sleep-related threat and negative cognitive distortion toward insomnia.
The association between bowel resection and the risk of nontyphoidal salmonella infection: a nationwide propensity score-matched cohort study
Authors: Yang Kuang-Tsu , Juang Sin-Ei , Hung Yao-Min , Wei James Cheng-Chung , Yip Hei-Tung , Chang Renin
Published: January 2021

Nontyphoidal salmonella (NTS) infection has a high mortality rate. Bowel resections affect gut microbiota and immune function, and the association between bowel resection and NTS infection in human beings has not been addressed. We conducted a nationwide propensity score (PS)-matched cohort study to clarify this association. Data from the Longitudinal Health Insurance Database of Taiwan were used to establish a case-cohort with bowel resections from 2000 to 2013. Informed consent was waived by the Institutional Review Board of China Medical University Hospital (CMUH104-REC2-115) because all personal identifying information used had been de-identified. Each case was matched with one control without any bowel resection according to age, gender, index date, and propensity score (PS). Cumulative incidences of and hazard ratios (HRs) for NTS infection development were analyzed. The incidence of NTS infection was greater in patients with a bowel resection than in the control group (2.97 vs. 1.92 per 10,000 person-years), with an adjusted hazard ratio (aHR) of 1.64 (95% CI = 1.08–2.48). The incidence of NTS infection increased significantly for cases with small bowel resections and right hemicolectomies. Age (31–40 and > 50 years), hypertension, chronic kidney disease, chronic obstructive pulmonary disease, and autoimmune diseases were significant risk factors of NTS infection. Stratification analysis revealed that patients without comorbidities were prone to NTS infection after bowel resections. The increased risk of developing NTS infection could be related to the bowel resection. Specific age groups and comorbidities also contribute to increased risk of NTS infection.
The nitrogen topdressing mode of indica-japonica and indica hybrid rice are different after side-deep fertilization with machine transplanting
Authors: Wang Xiaodan , Wang Yaliang , Zhang Yuping , Xiang Jing , Zhang Yikai , Zhu Defeng , Chen Huizhe
Published: January 2021

Determination of the optimal fertilization method is crucial to maximize nitrogen use efficiency and yield of different rice cultivars. Side-deep fertilization with controlled-release nitrogen, in conjunction with machine transplanting and subsequent topdressing, was applied to Indica–japonica hybrid rice ‘Yongyou1540’ (YY1540) and indica hybrid rice ‘Tianyouhuazhan’ (TYHZ). Four nitrogen treatments were applied in 2018 and 2019: traditional nitrogen application with quick-release nitrogen (T1), single-dose deep fertilization at transplanting with 100% controlled-release nitrogen (T2), and deep fertilization of 70% controlled-release nitrogen and topdressing of 30% quick nitrogen at tillering (T3), or at panicle initiation (T4). Side-deep fertilization reduced the fertilizer application frequency without causing yield loss, T4 enhanced the yield of YY1540 by increasing the number of productive tillers and number of spikelets per panicle compared with T1, T2 and T3. The yield of TYHZ showed no significant difference among treatments. The T4 treatment decreased the number of tillers at the tilling peak stage and increased the percentage productive tillers and number of differentiated spikelets. Compared with the other treatments, T4 increased dry matter accumulation and leaf area index during panicle initiation and grain ripening, and contributed to enhanced nitrogen uptake and nitrogen utilization in YY1540. On average, nitrogen uptake and utilization in YY1540 were highest in T4, but no significant differences among treatments were observed in TYHZ. Dry matter accumulation and nitrogen uptake from panicle initiation to heading of YY1540 were correlated with number of spikelets per panicle, but no significant correlations were observed for TYHZ. Supplementary topdressing with quick-release nitrogen at the panicle initiation stage was required to increase yield of indica–japonica hybrid rice, whereas single-dose deep fertilization with controlled-release nitrogen is satisfactory for the indica hybrid cultivar.
A toehold-mediated strand displacement cascade-based DNA assay method via flow cytometry and magnetic separation
Authors: Ju Ting , Zhai Xingwei , Liu Xinfeng , Han Kun
Published: January 2021

A novel Flow Cytometry Assay (FCA) that combines amplification cascade is proposed for the detection of EGFR T790M.
Metal–organic frameworks as advanced materials for sample preparation of bioactive peptides
Authors: Zhong Huifei , Li Yongming , Huang Yanyan , Zhao Rui
Published: January 2021

This minireview summarizes the recent advances in MOF-based affinity materials for the sample preparation of bioactive peptides.
Methanol reforming denitration over an integrated bifunctional CuZnOx–X–MnPdOz@Ni catalyst at low temperature
Authors: Xie Tianying , Cao Limei , Sun Wei , Yang Ji
Published: January 2021

Severe conditions involved in hydrogen storage and transportation limit the practical applications of hydrogen denitration reaction.
g-C3N4/metal halide perovskite composites as photocatalysts for singlet oxygen generation processes for the preparation of various oxidized synthons
Authors: Corti Marco , Chiara Rossella , Romani Lidia , Mannucci Barbara , Malavasi Lorenzo , Quadrelli Paolo
Published: January 2021

Singlet oxygen generated by g-C3N4/metal halide perovskite composites as photocatalysts in organic oxidation reactions.
Helpful correlations to estimate the pKaof coordinated HNO: a potential–pH exploration in a pendant-arm cyclam-based ruthenium nitroxyl
Authors: Perdoménico Julián , Ruiz Mariano M. , Osa Codesido Nicolás , De Candia Ariel G. , Marcolongo Juan P. , Slep Leonardo D.
Published: January 2021

The acidity of bound HNO in [Ru(Lpy)(HNO)]2+in water is the lowest reported to date. The pKa(HNO) values in several ruthenium-based species correlate with the redox potential of the {RuNO}6,7and {RuNO}7,8couples.
Carbon nanotube materials for electrocardiography
Authors: Kolanowska Anna , Herman Artur P. , Jędrysiak Rafał G. , Boncel Sławomir
Published: January 2021

Carbon nanotubes as 1D nanomaterials of excellent physicochemical characteristics bring hope to compete and eventually conquer traditional solutions in electrocardiography – the most powerful non-invasive diagnostic tools in cardiac disorders.
Synthesis of 2-ethoxycarbonylthieno[2,3-b]quinolines in biomass-derived solvent γ-valerolactone and their biological evaluation against protein tyrosine phosphatase 1B
Authors: Mu Xu-Yang , Wang Zhi-Jia , Feng Bo , Xu Lei , Gao Li-Xin , Satheeshkumar Rajendran , Li Jia , Zhou Yu-Bo , Wang Wen-Long
Published: January 2021

A series of 2-ethoxycarbonylthieno[2,3-b]quinolines were synthesized in the bio-derived “green” solvent γ-valerolactone and evaluated for their inhibitory activities against PTP1B, compound6adisplayed an IC50value of 8.04 ± 0.71 μM with 4.34-fold preference over TCPTP.
Bain and Nishiyama–Wassermann transition path separation in the martensitic transitions of Fe
Authors: Zhang L. H. , Cheng M. J. , Shi X. H. , Shuai J. W. , Zhu Z. Z.
Published: January 2021

The importance of martensitic transformations has led to tremendous efforts to explore the microscopic martensitic transition paths.