bluuebunny/crossref_metadata_2025 huggingface dataset:
Polyphenol oxidase from wheat bran is a serpin.
Authors: Yamasaki Yoshiki , Konno Haruyoshi , Noda Kazuhiko
Published: November 2019

Polyphenol oxidase (PPO; EC 1.10.3.2) was isolated from wheat bran by a procedure that included ammonium sulfate fractionation, batch adsorption by DEAE-cellulofine, CM-cellulofine column chromatography, DEAE-cellulofine column chromatography, preparative isoelectric focusing, adsorption on the membrane of a Vivapure Q Maxi H spin column, and heat treatment. These procedures led to 150-fold purification with 4.2% recovery. The PPO was homogeneous by SDS/PAGE. The relative molecular weight of the PPO was estimated to be 37,000 based on its mobility in SDS/PAGE. The isoelectric point of the PPO was 4.4. The K(m) values of the PPO for caffeic acid, chlorogenic acid, pyrocatechol, 4-methyl catechol and l-DOPA as substrates were 0.077, 0.198, 1.176, 1.667 and 4.545 mM. The PPO was strongly inhibited by tropolone. The K(i) value for tropolone is 2.2 x 10(-7) M. The sequence of the 15 N-terminal amino-acid residues was determined to be ATDVRLSIAHQTRFA, which was identical to those of serpin from Triticum aestivum and protein Z from Hordeum vulgare. The PPO strongly inhibited the activity of trypsin, which is an enzyme of serine proteases; 50% inhibition was observed with 1.5 x 10(-7) M PPO. The K(i) value for PPO is 2.3 x 10(-8) M. The wheat bran PPO should be a very important protein for protecting wheat against disease, virus, insect and herbivore damages by both the activities of PPO and protease inhibitor.
Association analysis of vitamin D receptor gene polymorphisms with bone mineral density in young women with Graves' disease.
Authors: Horst-Sikorska Wanda , Ignaszak-Szczepaniak Magdalena , Marcinkowska Michalina , Kaczmarek Marta , Stajgis Malgorzata , Slomski Ryszard
Published: November 2019

Graves' (GD) hyperthyroidism induces accelerated bone turnover that leads to decreased bone mineral density (BMD). The role of the VDR gene in predisposition to primary osteoporosis has been recognized. Recent studies show associations between the VDR gene polymorphisms and susceptibility to autoimmune diseases. Here we analyzed if VDR gene polymorphisms: BsmI, ApaI, TaqI, and FokI may predispose women with Graves' hyperthyroidism to BMD reduction or to disease development. The subjects were 75 premenopausal female Polish patients with GD and 163 healthy women. The genotyping was performed by the use of the restriction fragment length polymorphism analysis (RFLP). We studied the association of the VDR polymorphisms and their haplotypes with patients' BMD and also SNPs and haplotypes association with Graves' disease. We found a strong linkage disequilibrium for the BsmI, ApaI, and TaqI polymorphims that formed three most frequent haplotypes in Graves' women: baT (47.9%), BAt (34.9%), and bAT (16.4%). We did not show statistically significant association of analyzed VDR polymorphisms or haplotypes with decreased bone mineral density in Graves' patients. However, the presence of F allele had a weak tendency to be associated with Graves' disease (with OR=1.93; 95% CI: 0.97-3.84; p=0.058).
VDR gene polymorphisms do not predict the risk of decreased BMD in Polish women with Graves'. It may be speculated that the F allele carriers of the VDR-FokI polymorphism are predisposed to Graves' disease development.
High mobility group proteins stimulate DNA cleavage by apoptotic endonuclease DFF40/CAD due to HMG-box interactions with DNA.
Authors: Kalinowska-Herok Magdalena , Widłak Piotr
Published: November 2019

The DFF40/CAD endonuclease is primarily responsible for internucleosomal DNA cleavage during the terminal stages of apoptosis. It has been previously demonstrated that the major HMG-box-containing chromatin proteins HMGB1 and HMGB2 stimulate naked DNA cleavage by DFF40/CAD. Here we investigate the mechanism of this stimulation and show that HMGB1 neither binds to DFF40/CAD nor enhances its ability for stable binding to DNA. Comparison of the stimulatory activities of different truncated forms of HMGB1 protein indicates that a structural array of two HMG-boxes is required for such stimulation. HMG-boxes are known to confer specific local distortions of DNA structure upon binding. Interestingly, the presence of DNA strand cross-links formed by cisplatin or transplatin, which may somehow mimic distortions induced by HMG-boxes, also affects DNA cleavage by the nuclease. The data presented suggest that changes induced in DNA conformation upon HMG-box binding makes the substrate more accessible to cleavage by DFF40/CAD nuclease and thus may contribute to preferential linker DNA cleavage during apoptosis.
Alteration of O-specific polysaccharide structure of symbiotically defective Mesorhizobium loti mutant 2213.1 derived from strain NZP2213.
Authors: Turska-Szewczuk Anna , Pietras Hubert , Borucki Wojciech , Russa Ryszard
Published: November 2019

Mesorhizobium loti mutant 2213.1 derived from the wild-type strain NZP2213 by Tn5 mutagenesis showed impaired effectiveness of symbiosis with the host plant Lotus corniculatus (Turska-Szewczuk et al., 2007 Microbiol Res, in press). The inability of lipopolysaccharide (LPS) isolated from the mutant 2213.1 strain or de-O-acetylated LPS of the parental cells to inactivate phage A1 particles implicated alterations in the LPS structure. The O-specific polysaccharide of the mutant was studied by chemical analyses along with (1)H and (13)C NMR spectroscopy, which clearly confirmed alterations in the O-chain structure. 2D NMR data showed that the mutant O-polysaccharide consists of a tetrasaccharide repeating unit containing non-substituted as well as O-acetylated or O-methylated 6-deoxytalopyranose residues. Additionally, an immunogold assay revealed a reduced number of gold particles on the mutant bacteroid cell surface, which could result from both a diminished amount of an O-antigenic determinant in mutant LPS and modifications of structural epitopes caused by alterations in O-acetylation or O-methylation of sugar residues. Western immunoblot assay of alkaline de-O-acetylated lipophilic M. loti NZP2213 LPS showed no reactivity with homologous serum indicating a role of O-acetyl groups in its O-specificity.
Prolonged Methamphetamine-Induced Psychosis: Difference With Schizophrenia
Authors: Ahmadkhaniha Hamid Reza , Alavi Kaveh , Hadi Fatemeh
Published: November 2019

The main objective of the present study was to compare the distribution of underlying factors such as neurological soft signs, obstetric complications, and family history of psychiatric disorders between two groups of schizophrenic patients and patients with prolonged methamphetamine-induced psychosis. In a case-control study, 30 patients with prolonged methamphetamine-induced psychosis and 30 patients with schizophrenia were selected. Data were collected through a demographic questionnaire, the Buchanan and Heinrichs’ Neurological Evaluation Scale (NES), the Lewis-Murray’s Obstetric Complications Scale (LMOCS), and the Weissman’s Family History Screen (FHS). Mean scores of the neurological soft signs (±SD) in the two groups of schizophrenic patients and patients with prolonged methamphetamine-induced psychosis were 15.7±8.7 and 11.7±6.2, respectively (P=0.040), and a significant difference was observed in the sensory integration between the two groups (P=0.022). Obstetric complications revealed similar distributions in the two groups. Patients with prolonged methamphetamine-induced psychosis reported higher prevalence of alcohol and other substances use disorders (P=0.003 and P=0.001, respectively) in their close relatives; however, the distributions of other disorders were not statistically different between the two groups’ close relatives. Similarities and differences in certain aspects were observed between the two groups, suggesting susceptibility for psychosis in patients with prolonged methamphetamine-induced psychosis; yet we found diversities that distinguish the two disorders.
© 2019 Tehran University of Medical Sciences. All rights reserved.
Acta Med Iran 2019;57(4):235-243
The Effects of Chlorhexidine Dressing on Health Care-Associated Infection in Hospitalized Patients: A Meta-Analysis
Authors: WANG Hou-Xing , XIE Shu-Yuan , WANG Hao , CHU Hao-Kai
Published: November 2019

Background: To assess the effects of chlorhexidine dressing on health care-associated infection in hospitalized patients.
Methods: We searched for English-language published randomized controlled trials (RCTs) in Cochrane Library, EMBASE and PubMed between January 1998 and January 2018. We used meta-analysis to calculate the risk ratios (RRs) and 95% confidence intervals (CIs) of the data, and using the I2 assessment to summarize the heterogeneity of RCTs and the funnel plot and Egger regression test to evaluate publication bias.
Results: A total of 13 RCTs were included in our meta-analysis, including 7555 patients and 11,931 catheters. The effects of chlorhexidine dressing on the incidence of catheter-related bloodstream infections (CRBSIs) were reported in 13 RCTs, and the incidence of CRBSIs were 1.3% (80/6160) in the chlorhexidine group and 2.5% (145/5771) in the control group. We used a forest plot to determine the risk ratio (RR) of chlorhexidine dressing on the incidence of CRBSIs, and our results showed that chlorhexidine dressing significantly reduced the incidence of CRBSIs (RR 0.55, 95% CI 0.39-0.77, P<0.001). Moreover, we also analyzed the effects of chlorhexidine dressing on the incidence of catheter colonization and catheter-related infections (CRIs), and our forest plot results showed that chlorhexidine dressing significantly reduced the incidence of catheter colonization (RR 0.52, 95% CI 0.40-0.67, P<0.001) and the incidence of CRIs (RR 0.43, 95% CI 0.28-0.66, P<0.001) in hospitalized patients.
Conclusion: The use of chlorhexidine dressings for hospitalized patients significantly reduce the incidence of CRBSIs, catheter colonization and CRIs.
Effects of Occupational Stress on Blood Lipids, Blood Sugar and Immune Function of Doctors
Authors: WANG Wenjuan , REN Hui , TIAN Qiuye , TANG Chunling , MENG Wenjuan
Published: November 2019

Background: We aimed to investigate the effects of occupational stress on blood lipids, blood glucose and immune function of doctors.
Methods: In 2017, 1291 doctors (565 males, 726 females) in The First Hospital Affiliated with Harbin Medical University (Harbin, China) were enrolled based on the principle of convenience of sampling and cluster sampling. Questionnaires were used to investigate demographic characteristics and occupational stress related factors. Level of glycated hemoglobin was detected by immunoturbidimetric method. Concentration of triglyceride was determined by        glycerol phosphate oxidase end point method. Total cholesterol concentration in serum was determined by total cholesterol oxidase end point method. Concentration of serum immunoglobulin was detected by immunoturbidimetry.
Results: Levels of glycated hemoglobin and triglyceride in high tension group were higher than those in the low tension group. Levels of IgG and IgM in high tension group were lower than those in low tension group. The risk of elevated glycated hemoglobin levels in > 50-yr-old age group was higher than that of the =<35-yr-old age group. Those in the high coping strategy group was higher in the low coping strategy group. The risk of elevated total cholesterol levels in drinkers is 1.158 times that of non-drinkers. The risk of IgG concentration reduction in smokers was 0.428 times that of non-smokers. The risk of a decrease in IgA concentration in doctors with good sleep quality is 1.527 times that of those with poor sleep quality.
Conclusion: Occupational stress can lead to increased blood lipids and sugar levels as well suppression of immune function in doctors.
Clinical Efficacy of Physical Factors Combined with Early Psychological Intervention in Treatment of Patients with Chronic Limb Pain
Authors: QIN Liang , CUI Caihong , HUO Yuling , YANG Xincai , ZHAO Yuqian
Published: November 2019

Background: To investigate the clinical efficacy of physical factors combined with early psychological intervention in treatment of patients with chronic limb pain.
Methods: A total of 132 patients with chronic limb pain admitted to Affiliated Hospital of Hebei University, Baoding, China from June 2014 to June 2017 were enrolled. The patients were divided into control group (n=66) and observation group (n=66) according to the random number table method. Both groups of patients were treated with physical factors, and the patients in the observation group were also treated with early psychological intervention. PHQ-15 pain factor score, visual analog pain score (VAS score), Hamilton depression rating scale (HAMD) and clinical efficacy were compared between two groups.
Results: Difference of PHQ-15 pain factor score, VAS score and HAMD score between two groups before treatment were not statistically significant (P=0.091, 0.161, 0.078). At the end of treatment and at 8 weeks of follow-up, PHQ-15 pain factor score, VAS score, and HAMD score of observation group were lower than those of control group, and the differences were statistically significant (P=0.045, 0.014; 0.011, 0.025; 0.030, 0.015). Total clinical effective rates of observation group and control group were 92.43% and 86.37%, respectively, and the differences were statistically significant (P=0.019).
Conclusion: Compared with physical factors alone, combination of physical factors and early psychological intervention can significantly alleviate the pain and improve the depression of patients with chronic limb pain. It should be promoted in clinical practices.
Detection of Toxoplasma gondii in Cord Blood Samples from Neonates in Tehran, Iran
Authors: NAHVI Moloud , SHOJAEE Saeedeh , KESHAVARZ Hossein , SALIMI Mahboobeh , MOHEBALI Mehdi
Published: November 2019

Background: Toxoplasmosis is an infectious disease caused by the coccidian protozoan parasite Toxoplasma gondii. The infection is life-threatening in congenital form because of transmission of the parasite from mother to fetus. In order to investigate the prevalence of congenital toxoplasmosis, the present study was performed for detection of IgG and IgM antibodies in cord blood samples of newborns by ELISA method in Tehran, Iran.
Methods: This cross-sectional survey was carried out on 1000 cord blood samples collected from Shahid Mostafa Khomeini Hospital in Tehran, Iran in 2015. Sera were separated and evaluated for the presence of IgG and IgM antibodies against T. gondii by ELISA method. At the same time, whole cord blood samples were stored at -20 °C for complementary PCR test.
Results: From 1000 cord blood serum samples 198 cases (19.8%) were positive for anti T. gondii IgG antibody. IgG positive samples were examined for IgM antibody, among them 1 sample had borderline levels of IgM antibody. PCR was performed for this sample but no positive result was seen.
Conclusion: Although congenital toxoplasmosis is of importance, no acute form of infection was seen in pregnant women in this study.
Managing Chronic Low Back Pain with Modified Package of Exer-cise Therapy Enriched by Psychological Interventions in Computer Based Workers: A New Approach in Developing Countries
Authors: HOSSEINI Lida , GHAYOUR NAJAFABADI Mahboubeh , MEMARI Amirhossein , KORDI Ramin
Published: November 2019

This article is a Letter to the Editor and does not include an Abstract.
Electromagnetic Fields Exposure from Power Lines and Human Fertility
Authors: ESMAILZADEH Sedigheh , AGAJANI DELAVAR Mouloud , GHOLAMIAN Sayyed Asghar , AHMADI Amirmasoud , HOSSEINPOUR HAYDARI Fatemeh , POURALI Mahbobe
Published: November 2019

This article is a Letter to the Editor and does not include an Abstract.
Prevalence of Staphylococcus Aureus Isolated from Hamburger Samples in Tehran and their Antibiotic Susceptibility
Authors: Momtaz Bokharaei Negin , Rajabi Zahra , Soltan Dallal MohammadMehdi
Published: November 2019

Introduction:Staphylococcal food poisoning is one of the most common food-borne diseases. The antibiotic resistance of Staphylococcus aureus has been reported globally. Today, strains of Staphylococcus aureus in food have become a problem in clinical infections and are considerde as a serious public health concern.
Methods: A total of 100 samples were tested in the laboratory; including 75 handmade and 25 industrial hamburger samples. The Staphylococcus aureus samples were isolated and identified according to the National Iranian Standard No. 6806-3. Antibiotic resistance was determined by disk diffusion method and minimum inhibitory concentration was performed. In this study, the antibiotics penicillin, ciprofloxacin, erythromycin, clindamycin, chloramphenicol, cefoxitin, trimethoprim-sulfamethoxazole, tetracycline, gentamicin, vancomycin, and oxacillin were used.
Results: Of 100 analyzed hamburger samples, 39 samples were infected with Staphylococcus aureus (23 industrial and 16 ­ handmade samples). All isolates of Staphylococcus aureus (100%) were susceptible to gentamicin and vancomycin antibiotics.
Conclusions: Due to the importance of Staphylococcus aureus, as the supergene antigen and its role in causing food poisoning, contamination of foodstuffs with Staphylococcus aureus can threaten the consumers' health.
Self-management Determinants in Kidney Transplant Recipients according to the Health Belief Model
Authors: Farnia Farahnaz , Shafiezadeh Mehdi , Javadi Mostafa
Published: November 2019

Introduction: Kidney transplant recipients, as chronic patients, need lifelong self-management. Therefore, identification of the effective factors is helpful in strengthening the individuals' adherence and planning self-management in the post-transplant period. However, the empirical research on this area is limited. The purpose of this study was "Explaining self-management determinants in kidney transplant recipients according to the health belief model".
Methods: This qualitative content analysis was conducted in Kohgiluyeh and Boyer Ahmad province. The participants were included in the study by purposive and theoretical sampling methods until theoretical and classical saturations were achieved. The data were collected and analysed using 24 semi-structured interviews by 19 participants (14 transplant recipients, 4 family members, and a patient with a history of transplant rejection). The MAXQDA10 software was used to organize and manage the data.
Results. After analysing the data, 264 primary conceptual codes were extracted. The findings were reported in 20 sub- categories and in accordance with six categories of the health belief model (perceived susceptibility, perceived severity, perceived benefits, perceived barriers, self-efficacy, and cues to actions).
Conclusion. The findings of this qualitative study provided a profound understanding over the experiences of kidney transplant recipients and their family members about self-management determinants. The findings confirmed the health belief model constructs and its application in predicting these patients' self-management. In addition, the findings can be  a guide for health planners and policy makers to select the suitable interventions and strategies to improve the adherence to recipients' self-management.
Meslek Lisesi Öğretmenlerinin Öğretmen Profesyonelliği ve Öğretmen Liderliğine İlişkin Algılarının İncelenmesi
Authors: ÇETİN Münevver , ÖZALP Uğur
Published: November 2019

Bu araştırmanın temel amacı
meslek lisesi öğretmenlerinin öğretmen profesyonelliği ve öğretmen liderliğine
ilişkin algılarını incelemektir. Olgu bilim deseninde tasarlanmış bu nitel
araştırmanın çalışma grubunu 2017-2018 eğitim-öğretim yılında İstanbul ili
Eyüpsultan ilçesinde çalışan toplam 34 öğretmen oluşturmuştur. Araştırma
verileri yarı yapılandırılmış görüşme formuyla toplanmıştır. Elde edilen
veriler ile içerik analizi yapılarak bulgular raporlaştırılmıştır. Öğretmen
liderliği için sosyal ve kültürel etkinlikler gerçekleştirilmesi gerektiği,
nitelikli hizmet içi eğitimler düzenlenmesi gerektiği ve iletişimin
güçlendirilmesi gerektiği sonucuna ulaşılmıştır. Öğretmen liderliğinin
öğretmenlerin alınan kararlardan birinci derecede etkilenmesinden dolayı
gerekli olarak algılanmasının yanında karar almayı zorlaştırmasından dolayı
gereksiz olarak algılandığı tespit edilmiştir. Profesyonel öğretmenlerin
yaratıcı ve üretici etkinlikler tasarlayan, lisans düzeyinde eğitime sahip,
özerk ve öz denetimli bireyler olarak algılandığı sonucuna ulaşılmıştır.
Psikolojik Sermayenin Bireysel Performansa Etkisi: Hemşireler Üzerinde Bir Araştırma
Authors: KUŞCU KARATEPE Hilal , KUŞCU Fatma Nuray , KARAMAN Mesut
Published: November 2019

Bu çalışma
sağlık hizmetlerinin önemli bir parçası olan hemşirelerde psikolojik sermaye
düzeylerinin belirlenmesi ve psikolojik sermayenin bireysel performansa
etkisini ortaya koymak amacıyla yapılmıştır. Araştırma Malatya’da bir kamu
hastanesinde 150 hemşireden elde edilen veriler doğrultusunda
gerçekleştirilmiştir.  Veri toplama araçları
olarak yararlanılan ölçekler  Luthans
vd., (2007)’nin geliştirdiği; Çetin ve Basım (2012)’ın Türkçeye adaptasyon
çalışmasını yaptığı Psikolojik Sermaye Ölçeği ile Schepers (2005)’ın geliştirip
Özpehlivan (2015)’nın geçerlilik güvenirlik çalışmasını yaptığı Bireysel
Performans Ölçeği’nden yararlanılmıştır. Verilerin analizinde sayı-yüzdelik
hesaplama, frekans, t testi, tek yönlü varyans analizi, pearson kolerasyon
analizi, basit ve çoklu regresyon analizi yöntemleri uygulanmıştır. Bu
çalışmada psikolojik sermayenin ve alt boyutlarından öz yeterlilik alt
boyutunun bireysel performansı istatiksel açıdan anlamlı ve pozitif yönde
etkilediği belirlenmiştir.
Potencialidad del clúster turístico en el municipio de Pamplona, región nororiental de Colombia
Authors: Ortiz Flórez Jazmín Solange , Cancino Susan Elsa , Cancino Escalante Giovanni Orlando
Published: November 2019

El presente artículo tiene como objetivo realizar un diagnóstico del sector turístico del municipio de Pamplona (Colombia) con la finalidad de identificar la posibilidad de la implementación de un clúster turístico en la región. Se llevó a cabo un análisis de los conceptos teóricos alrededor del turismo y del clúster, así como un estudio cuya ruta metodológica se apoyó en el empleo de encuestas y fuentes secundarias. Los resultados indican la existencia de atractivos turísticos, una amplia red de infraestructura, instituciones de apoyo, baja inversión, falta de cooperación y mano de obra poco calificada. Se resalta la necesidad de consolidar el clúster turístico del municipio bajo una estructura organizativa coherente, con el fin de reducir los obstáculos y estimular la participación de los actores.
Live Streaming Platforms and the Critical Discourse About Video Games
Authors: Felczak Mateusz
Published: November 2019

This work concerns online streaming platforms centered on broadcasting video games-related content, such as Twitch.tv and Douyu.com. The aim of this article is two-fold: first, to point out the possible research methods regarding online streaming services as mediums for video game criticism, and second, to assess the potential of these platforms for generating critical discourse about video games. The methodology of the research is based on Mia Consalvo’s take on paratextual frameworks and Veli-Matti Karhulahti’s notions of interview and play frames regarding the activity of video games streaming. In this article, the initial characterization of Twitch.tv and Douyu.com is followed by an analysis on how the work of streamers as content creators can influence the reception of video games. Afterwards, a reading of analyzed phenomena based on paratextual framework is proposed. The last section juxtaposes the initial findings with the perspective of changing viewership figures in relation to selected ludic properties of games. In conclusion, it is stated that the analyzed streaming platforms reflect the proliferating crowdsource trends in the video game industry, with forms of interacting with the product at various stages of its development cycle establishing new practices of talking in and about video games.
The current state and promising innovative directions to development methods for bioimplant sterilization
Authors: Rozanov V. V. , Matveychuk I. V.
Published: November 2019

We have analyzed the state-of-the-art methods for sterilization of bone implants. The problem of finding effective bioimplant sterilization methods is still far from its optimal solution and remains as urgent as before. The factors limiting further development of the main biomaterial sterilization methods include limitations related to each existing method and the use of technologies with sterilizing effect. Comparative analysis of the main techniques for bioimplant sterilization that are used in medical and biological areas (treatment with ethylene oxide, radiation, wet warmth, liquid media, and ozone) allows for a conclusion on the advantages of the radiation sterilization. However, the choice is challenged by the dilemma: higher radiation dose would increase the sterilization effect, but at the same time can lead to multiple morphological abnormalities in the tissues, deterioration of their mechanical characteristics, destruction of morphogenetic proteins and consequently to lower efficacy of the reparative bone formation. As a  result, the material can become unsuitable for clinical use. One of the real approaches to solve this problem is to use as low absorbed radiation dose as possible during irradiation of biomaterials, at least to 15 kGy. The developments made by the authors within the last years have shown that such a  result can be achieved by the use of combines sterilization techniques based on combines effects of a  number of physical and chemical factors on the biomaterial being sterilized. Mutual enhancement of the sterilizing effects of these factors creates prerequisites for their synergy, whereby the intensity of each factor can be reduced. This makes it possible to decrease the degree of harmful adverse events associated with each individual factor with higher total effect. The search for innovative solutions for the urgent problems of the bone bioimplant sterilization, for the development of the state-of-the-art health-sparing technologies can be successful only with unification of the efforts by specialists from related sciences. This would allow for creating of breakthrough technologies for sterilization and for optimization of this procedure with achievement of its high efficacy.
Os efeitos da musicalização para o desenvolvimento musical em bebês de zero a dois anos
Authors: Ruas José Jarbas , Vilarinho Fabiana De Freitas Angulo
Published: November 2019

O presente artigo investiga os efeitos provocados pela música em bebês de zero a dois anos e as possibilidades para o seu desenvolvimento físico, social e afetivo. Realizamos estudo bibliográfico apoiando-nos em Delalande, Gordon, Beyer, Ilari, dentre outros autores. Conceituamos bebê, música e musicalização numa perspectiva baseada no estágio sensório-motor, segundo Jean Piaget. Discorremos sobre a Teoria do Desenvolvimento Musical e a estimulação musical. Delineamos aspectos relevantes das práticas e estimulações musicais, suas prováveis causas e efeitos, salientando a influência da música no desenvolvimento infantil e o vital papel familiar e escolar nesse processo.
The Animalistic Genre in the Works of Barbara Frischmuth
Authors: Trofimova Liudmila , Barova Alyona
Published: November 2019

The purpose of the article is to consider the originality of the art world of the contemporary Austrian writer Barbara Frischmuth who created in the novel Mistress of the Animals a unique "animalistic context". Frischmuth explores human nature through the images of the animal world, in connection with which the author of the article adheres to the term "human-animal studies". "Human-animal studies" in the works of the writer have a mythological nature, with the help of which Frischmuth illustrates the modern world. The relevance of the study is dictated by the inadequate exploration in homeland literary studies of the Austrian writer B. Frischmuth. Hence, considering the work of the Austrian writer in this context, this research is an attempt to pastose  the generalised characteristic of "animalier art". The undertaken study considers the understudied topical problems of modernity, given the literary experience of Barbara Frischmuth. In the animal work Frishmuths"anthropological" and "zoological" are transformed in a specific synthesis; this duality characterises animal characters in her works, and would become a hallmark of her work.
An Ecological Interpretation of the Return of Mom-and-Pop Stores in South Korea: A Sustainable Commercial Format-Enhancing Urban Diversity or Just a Temporary Trend?
Authors: Kim Juil
Published: November 2019

After globalization, South Korea’s retail landscape has been saturated with large-scale, corporate type retails. However, recently new commercial districts composed of small retail units are returning and bringing about a change. This study sought to take note of this phenomenon and identify its features and meanings from the perspective of urban ecology. A density-distribution analysis was conducted to investigate how they were forms, and an analysis of traces on the Internet and an analysis on the types of businesses were done to identify sociocultural characteristics. Results showed that they had similar type of locations and growth patterns, that they harmoniously congregated in a form of smaller-individual stores, and that their use of similar names for their stores had an impact of branding their entire districts. It was also noted that a shared culture through social networking services served as a growth boost for their unfavorable location. The spontaneous formation of such commercial districts can be an outcome of an urban ecological process geared toward blank niches burgeoning in the current retail structure of Korea. The causes and conditions found in the cases reveal meaningful policy implications for cities facing the same urban crisis diversity.
PROFESIONALISME PUSTAKAWAN DALAM LAYANAN INFORMASI
Authors: Anawati Sri
Published: November 2019

Advances in science and technology are increasingly causing increased the need
and demand pemustaka. Here librarians are required to be able to answer these
challenges, in order to meet the needs user. Librarian is a profession that requires education or training to manage the library. The professionalism of librarians should be increased because the librarian is an important element in the library. If the library does not want to be left pemustaka and want to continue to progress and evolve, the librarian should develop a professional attitude in order to create a positive image. A professional librarian who not only required to master the skill and technique of the library, but his soul felt called to become a librarian and aware that the profession of librarian is the servant of the library patron.

Keywords: librarians , professionalism , service information
Suplementação de colina em dietas para codornas de corte (Coturnix coturnix coturnix)
Authors: Nepomuceno R.C. , Sá N.L. , Freitas E.F. , Lima R.C. , Aguiar G.C. , Figueiredo C.W.S. , Silva C.P.
Published: November 2019

Os manuais de exigência nutricionais para codornas são baseados em estudos com codornas japonesas, e há poucas publicações que pesquisaram a exigência de vitaminas para codornas europeias. Objetivou-se avaliar os efeitos da suplementação de colina na alimentação de codornas de corte em 2 experimentos. No primeiro, foram alojadas 480 codornas machos e fêmeas, com 7 dias de idade e peso médio de 43 ± 0,22 g, distribuídas em delineamento inteiramente casualizado, com 6 tratamentos e 5 repetições de 16 aves. No segundo, foram alojadas 540 codornas machos e fêmeas, com 1 dia de idade peso médio de 8 ± 0,33 g, distribuídas em um delineamento experimental inteiramente casualizado com 6 tratamentos e 5 repetições com 18 aves cada. Em ambos os experimentos, os tratamentos consistiram de rações contendo 0, 0,025, 0,050, 0,075, 0,100 e 0,125% de cloreto de colina, correspondendo a 0, 150, 300, 450, 600 e 750 mg / kg de colina suplementada, respectivamente. As rações foram calculadas para serem isoenergéticas e isonutrientes, com exceção dos níveis de colina. Ao final do período experimental, foram avaliados o consumo de ração, ganho de peso, conversão alimentar, rendimento de carcaça, peito, coxa e sobrecoxa, proporção de gordura abdominal e do fígado e a composição do fígado. Os dados foram submetidos à análise de regressão. Em ambos experimentos, não houve efeito significativo da suplementação de colina sobre o desempenho, características de carcaça e composição do fígado. No entanto, na comparação entre os sexos houve diferença significativa no rendimento de carcaça, rendimento de peito no experimento 1, no peso relativo do fígado em ambos os experimentos e na quantidade de gordura no fígado apenas no experimento 1. Conclui-se que quando a quantidade de colina aportada pelos ingredientes for de 1.546 mg de colina/ kg de ração, a suplementação dessa vitamina não é necessária para codornas de corte.
Análisis de las preferencias de los consumidores españoles hacia la miel de producción social y ambientalmente responsable frente a la de Comercio Justo
Authors: Sama C. , Crespo-Cebada E. , Díaz-Caro C. , Mesías F.J.
Published: November 2019

En las últimas décadas se ha producido un notable aumento en la concienciación del consumidor por alimentos de calidad, y producidos de una forma social y ambientalmente responsable. El ámbito agroalimentario presenta uno de los mayores mercados en lo que se refiere a este tipo de producción ética-sostenible, como el Comercio Justo, y abre nuevas oportunidades para los productores a través de otros sistemas como la producción social y ambientalmente responsable. El objetivo de este trabajo consiste en determinar los condicionantes en el consumo de alimentos de Comercio Justo, además de la disposición a comprar alimentos social y ambientalmente responsable para la población de Extremadura (España). Para ello se ha aplicado un cuestionario a una muestra representativa (n=461) de la población extremeña en términos de edad y sexo. El análisis empírico consta de un análisis conjunto para determinar las preferencias hacia ambos tipos de producto y un modelo probit para determinar qué factores inciden sobre la probabilidad de consumo/disposición a consumir. Los resultados muestran que los consumidores prefieren los dos tipos de producción responsable a la convencional. Asimismo, el perfil de individuo que tiene una mayor probabilidad de consumir alimentos de comercio justo es mujer, con conocimiento previo acerca del Comercio Justo y con sensibilidad hacia los problemas laborales y ambientales. Finalmente, las mujeres, las rentas más altas, y los consumidores sensibilizados y que eran consumidores de Comercio Justo, son las principales características que influyen positivamente sobre la disposición a consumir alimentos social y ambientalmente responsables.
A FINANCIAL INCLUSION INDEX FOR INDONESIA
Authors: Hanivan Hilman , Nasrudin Nasrudin
Published: November 2019

Numerous studies have constructed financial inclusion indexes for Indonesia, using amultidimensional approach. However, there is a problem with the methodology, whichassumes that all the dimensions play the same role in defining financial inclusion,since they are based on equal weighting criteria. This paper aims to obviate concernswith the methodology by developing a more empirically based index, namely, aweighted multidimensional index of financial inclusion based on two-stage principalcomponent analysis. In other words, we endogenize the weights. We find that usage isthe most important dimension in defining financial inclusion in Indonesia, followedby availability and access.
As Industry Changes, So Does Petroleum Engineering Education
Authors: Feder Judy
Published: November 2019

As the oil and gas industry becomes increasingly complex, the requirements for petroleum engineers—and petroleum engineering education—are changing.

“Petroleum engineering jobs in the future are likely to be smaller in number and radically different from those of today,” wrote Nathan Meehan, president of Gaffney, Cline and Associates and 2016 SPE President, in paper SPE•194746, The End of Petroleum Engineering As We Know It. “The next generation of petroleum engineers will have to address demands for sustainability, lower carbon intensity, and needs for radical productivity improvements, which only artificial intelligence (AI) and digital can drive. This suggests that we will need to revisit university education for petroleum engineers and all aspects of career development and•training.”

Petroleum engineering education reflects the E&P industry it serves. Training of competent graduates with both the domain and digital knowledge to immediately contribute when they join the industry has become more complicated as expectations change. What are petroleum engineering schools doing to adapt to these changes, and how are they helping their students navigate a future career in the oil and gas industry?

Balancing Theory and Practical Application

A survey by Ryder Scott Petroleum Consultants identified the need to modify the skills and knowledge currently taught in academic institutions during undergraduate study. Ryder Scott surveyed clients who were industry managers or supervisors with direct experience with newly minted petroleum engineering graduates (fewer than 5 years of experience). The survey asked participants—engineering managers in the oil and gas reserves sector—their opinions regarding the preparedness of recent graduates entering the workforce (Fig. 1).

Generally, the respondents were satisfied with the amount of theory being taught, but not with the amount of practical application of petroleum engineering principles. The consensus was that curricula should be kept the same in terms of courses, but modernized to include the practical application of new technologies.

This presents a significant challenge for engineering education programs whose already-full curricula to meet requirements of the Accreditation Board of Engineering and Technology (ABET) leave little room for additional coursework, labs, or field work. The current requirement for a BS degree in petroleum engineering at most schools is 130-plus credit hours. Yet, in addition to fundamentals, today’s graduates are expected to be fluent in data analytics, machine learning, and data sciences and to understand concepts such as cyber security and physical security. In addition, many are expected to use their subsurface engineering skills to plan and design carbon sequestration solutions.

“We teach students the fundamentals of petroleum engineering,” said Jeff Spath, SPE 2014 President and head of the Texas A&M University Petroleum Engineering Department. “But more importantly, we teach them to solve petroleum engineering problems.”
Technology Focus: Production and Facilities (December 2019)
Authors: Frankiewicz Ted
Published: November 2019

Technology Focus

Innovation is alive and well in our industry, as evidenced by this year’s feature selections. The major thrust of papers relating to production and facilities continue to be big data analytics, process and field optimization, piping and pipelining, and the performance of inflow-control devices (ICDs and autonomous ICDs, or AICDs).

For ICDs and AICDs, publications have evolved from descriptions of the devices and how they work to field reports of their successful implementation. As with most new technologies, their degree and frequency of success are rapidly improving. Progress is exemplified by efforts to couple ICD/AICD installations with reservoir simulation (paper SPE 193364), thus giving technologists the ability to develop and digitally test placement models for ICD/AICD devices.• One paper (SPE 195450) reports on the completion of three wells in one formation. The well with ICDs outperformed the wells with a slotted liner or•perforations. •

For big data analytics, novel development work continues to focus on improving algorithms for directing preventative maintenance and risk-based inspections with fewer false-positive alarms. This technology has even begun migrating from the research and development stage by operators to use by service companies. One step-out application of big data analytics is the use of autonomous ground vehicles for the monitoring of buried onshore pipelines (paper SPE 192773). This allows for much more sophisticated pipeline inspections with far less health, safety, and environmental exposure.•

A project-management paper deserving attention describes a successful multiteam project with emphasis on interdisciplinary communication. The objective was to construct and start up a large liquefied natural gas train. The project involved 370 deliverables with over 7,000 unique line items (paper SPE•191984).

Other papers describing creative new technology include the testing of nano-paint-aided electromagnetic pigging of pipelines and production tubing (paper SPE 196112), a tetherless autonomous device capable of traveling with or against production flow to accomplish pigging and inspection (paper SPE• 195709), and the use of a solid propellant accumulator for activating blowout preventers (paper OTC 29151). These and a number of other publications are great examples of creative work on nascent technologies to improve all aspects of oil and gas production.

Recommended additional reading at OnePetro: www.onepetro.org.

SPE 191984 Facility Operations Readiness and Project Startup Support and Integration by Michael Maher, ConocoPhillips

SPE 196112 Nanopaint-Aided Electromagnetic Pigging in Pipelines and Production Tubing by Ningyu Wang, The University of Texas at Austin, et al.

OTC 29151 Solid Propellant Accumulators—Next-Generation Hydraulic•Power Source for Cold-Weather BOPs by Nazareth Bedrossian, Bastion Technologies
Technology Focus: Bits and Bottomhole Assemblies
Authors: Mensa-Wilmot Graham
Published: November 2019

Technology Focus

Drilling vibration continues to preoccupy the industry’s attention, primarily because of its known detrimental effects on operational efficiency, downhole tool reliability, cycle time, and project cost. In addition to drill bits and bottomhole assemblies that have been known to initiate vibrations, researchers have identified several other conditions and factors with similar contributions to vibration initiation. Vibration challenges, if effectively addressed, will have strong positive effects on performance.

The debate on whether the industry is doing enough to address vibrations continues to linger. This discussion must be put to rest because the fact is that researchers are passionately obsessed with this challenge. Presently, the focus has shifted from mere awareness to efforts that seek to establish a deeper understanding of this subject. In addition to identifying and characterizing different vibration modes, the current emphasis also delves into their sources. Knowledge gained from these efforts, particularly awareness of the different factors contributing to vibration, is driving development of holistic solutions to the challenge.

Numerous initiatives, including downhole tool development for data acquisition, big data and analytics, innovative modeling techniques, and calibration and optimization of modeling results, have been deployed in this effort. Positive results with measurable effects in field operations have been achieved. In all cases, development of effective solutions must start with appropriate characterization and identification of the specific modes of vibration. Its sources must also be established so that developed solutions can be tailored for maximum effectiveness.•

The holistic approach has identified borehole spiraling, especially in long lateral sections, as a primary source of vibrations and other drilling challenges. Solutions to address this challenge are currently in development. Yes, we continue to seek effective solutions to address the problem. However, we always encounter new challenges as we drill deeper into harsher environments, increase dogleg-severity requirements, and extend our departures while also drilling highly heterogeneous formations. We cannot stand still; we must keep moving ahead.

Recommended additional reading at OnePetro: www.onepetro.org.

SPE 196020 Case Studies: Optimizing BHA Performance by Leveraging Data and Advanced Modeling by Mojtaba Shahri, Apache, et al.

SPE 194120 Mitigation of Multifrequency Stick/Slip by Zhijie Sun, Halliburton, et al.

SPE 194150 Industry’s First PDC Bit Gauge Design for Improved Tracking In North American Horizontal Wells by Reed Spencer, Baker Hughes, a GE Company, et•al.
Silencing of the DJ-1 (PARK7) gene sensitizes pancreatic cancer to erlotinib inhibition
Authors: He Xiangyi , Sun Yunwei , Fan Rong , Sun jing , Zou Douwu , Yuan Yaozong
Published: November 2019

Background:
The epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor erlotinib, in combination with gemcitabine, has been shown to be a promising therapy in the treatment of pancreatic cancer. Our previous study showed that DJ-1 promotes invasion and metastasis of pancreatic cancer cells by activating SRC/ERK/uPA. The aim of this study was to evaluate whether silencing DJ-1 expression can sensitize pancreatic cancer cells to erlotinib treatment.
METHODS:
Knockdown of DJ-1 expression, combined with erlotinib treatment, was performed in the pancreatic cancer cell lines BxPC-3, PANC-1 and MiaPACa-2. Cell proliferation was assessed with the CCK-8 assay and BrdU incorporation, while apoptosis was measured using terminal deoxynucleotidyl ransferase (TdT)-mediated dUTP nick-end labeling. RAS activity and activation of the downstream pathways involving AKT and ERK1/2 were explored to determine the underlying mechanism.
RESULTS:
Knockdown of DJ-1 expression accelerated erlotinib-induced cell apoptosis, and improved the inhibitory effect of erlotinib on pancreatic cancer cell proliferation in vitro and in xenograft tumor growth in vivo . Knockdown of DJ-1 decreased K-RAS expression, membrane translocation, and activity in BxPC-3 cells. Knockdown of DJ-1 also decreased K-RAS, H-RAS, and N-RAS expression in PANC-1 and MiaPACa-2 cells. Knockdown of DJ-1 synergistically inhibited AKT and ERK1/2 phosphorylation with erlotinib in pancreatic cancer cells.
CONCLUSIONS:
DJ-1 may activate the RAS pathway, reinforcing erlotinib drug resistance. Blocking DJ-1 in combination with the EGFR tyrosine kinase inhibitor erlotinib may be an attractive therapeutic target in pancreatic cancer.
Abundance tracking by long-read nanopore sequencing of complex microbial communities in samples from 20 different biogas/wastewater plants
Authors: Brandt Christian , Bongcam-Rudloff Erik , Müller Bettina
Published: November 2019

Anaerobic digestion (AD) has long been critical technology for green energy, but the majority of the microorganisms involved are unknown and not cultivable, which makes abundance tracking difficult. Developments in nanopore sequencing make it a promising approach for monitoring microbial communities via metagenomic sequencing. For reliable monitoring of AD via long reads, a robust protocol for obtaining less fragmented, high-quality DNA, while preserving bacterial composition, was established. Samples from 20 different biogas/waste-water reactors were investigated and a median of 20 Gb sequencing data per flow cell were retrieved for each reactor. Using the GTDB index allowed sufficient characterisation of abundance of bacteria and archaea in biogas reactors. A dramatic improvement (1.8- to 13-fold increase) in taxonomic classification was achieved using the GTDB-based index compared with the RefSeq index. Ongoing efforts in GTDB to achieve more phylogenetically coherent taxonomic species definitions, including meta-assembled genomes, give a clear advantage over conventional classification databases such as RefSeq. Unlike conventional 16S rRNA studies, metagenomic read classification allows abundance of the unknown microbial fraction to be monitored.