bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Propofol versus Inhalation Anesthetics on Survival after Oncological Surgery
Authors: Jansen Laura , Dubois Bente F. H. , Hollmann Markus W.
Published: November 2022

Every year, 19.3 million patients worldwide are diagnosed with cancer. Surgical resection represents a major therapeutical option and the vast majority of these patients receive anesthesia. However, despite surgical resection, almost one third of these patients develop local recurrence or distant metastases. Perioperative factors, such as surgical stress and anesthesia technique, have been suggested to play a role to a greater or lesser extent in the development of recurrences, but oncology encompasses a complicated tumor biology of which much is still unknown. The effect of total intravenous anesthesia (TIVA) or volatile anesthesia (VA) on survival after oncological surgery has become a popular topic in recent years. Multiple studies conclude in favor of propofol. Despite the a priori probability that relevant differences in postoperative outcomes are due to the anesthesia technique employed, TIVA or VA, is extremely small. The existing literature includes mainly hypothesis-forming retrospective studies and small randomized trials with many methodological limitations. To date, it is unlikely that use of TIVA or VA affect cancer-free survival days to a clinically relevant extent. This review addresses all relevant studies in the field and provides a substantiated different view on this deeply controversial research topic.
The Postsurgical Clavien–Dindo Classification in Minor Surgery Can Improve Perception and Communication (Investigation on Blepharoplasty)
Authors: Eylert Gertraud , Wolfsberger Christina , Reischies-Meikl Frederike , Winter Raimund , Dong Susan , Michelitsch Birgit , Kamolz Lars-Peter , Lumenta David Benjamin
Published: November 2022

The postsurgical Clavien–Dindo classification in minor surgery can improve perception and communication (Investigation on Blepharoplasty). Background: Minor surgery lacks a standardized postoperative complication classification. This leads to the presentation of inaccurate postsurgical complication rates and makes comparisons challenging, especially for patients seeking information. This study aims to evaluate a standardized five-step complication grading system (Clavien–Dindo Classification, CDC) on the example of blepharoplasty, which is the most performed minor aesthetic surgery worldwide. Methods: A retrospective observational exploratory study of patients (N = 344) who received a bilateral upper eyelid blepharoplasty under local anesthesia from the same surgical staff was performed. Data were retrieved from the electronic patient record: the CDC grading and the surgeon-reported complications (N = 128) at the first follow-up on day 7. In addition, a telephone survey with patients (N = 261) after 6 months was performed, which consisted of 7 complication-related yes/no questions. Results: Based on the CDC, 41.6% of patients were classified as having no complications, and 58.4% had one. Furthermore, 1 patient (0.3%) received a revision under general anesthesia (CDC IIIb), 18 patients (5.2%) were re-operated under local anesthesia (CDC IIIa), 23 patients (6.7%) required pharmacological intervention (CDC II), and 159 patients (46.2%) had a complication from the normal postoperative course and received supportive treatment (CDC I). Moreover, 90.5% of the mentioned complications accounted for Grade I and II; 94% of the patients subjectively experienced no complications; 51% of patients were pleased with the surgery even though a complication occurred according to the CDC; 34% of complications escaped the awareness of the surgeon. Conclusions: Grade I and II complications occurred frequently. Complications escaped the perception of the patients and surgeons. The classification identifies a wide variety of postsurgical complications and allows a standardized comparison in minor surgery objectively. Potential: The CDC in minor procedures can improve the (institutional) preoperative communication with patients regarding potential postoperative expectations. Furthermore, the classification can be a useful tool to detect complication-related costs, identify insurance-related requests, and support evidence in medicolegal disputes. The example of blepharoplasty can be translated to various other and even less invasive procedures.
Can EU Bonds Serve as Euro-Denominated Safe Assets?
Authors: Bletzinger Tilman , Greif William , Schwaab Bernd
Published: November 2022

A safe asset is of high credit quality, retains its value in difficult times, and is traded in liquid markets. We show that bonds issued by the European Union (EU) are widely considered to be of high credit quality, and that their yield spread over German Bunds remained contained during the 2020 COVID-19 pandemic recession. Recent issuances and taps under the EU’s SURE and NGEU initiatives helped improve EU bonds’ market liquidity from previously low levels, while also reducing liquidity risk premia. Eurosystem purchases and holdings of EU bonds did not impair market liquidity. Currently, an obstacle to EU bonds achieving a genuine euro-denominated safe asset status, approaching that of Bunds, lies in the one-off, time-limited nature of the EU’s COVID-19-related policy responses.
The Insights, “Comfort” Effect and Bottleneck Breakthrough of “E-Commerce Temperature” during the COVID-19 Pandemic
Authors: Yang Yixing , Ma Yanan , Wu Gang , Guo Qian , Xu Hongbo
Published: November 2022

The impact of the COVID-19 pandemic on fresh food e-commerce has led to a loss of consumers, and “e-commerce temperature” is seen as an important means of alleviating consumer dissatisfaction and retaining consumers. To explore the connotation and effect of it, and to break through possible “comfort” bottlenecks, we used online reviews of the Jingdong fresh food platform as research data, mined the characteristics of “e-commerce temperature” with the help of the LDA topic model, and evaluated the mechanism of “e-commerce temperature” on consumer satisfaction during the pandemic by using quasi-natural experiments and Word2vec-based sentiment analysis. The results show that “e-commerce temperature” has five connotations of logistics commitment, humanized delivery, health pledge, pandemic perseverance, and consumer care, which can effectively mitigate the loss of consumer satisfaction. Interestingly, we found that the “e-commerce temperature” has a limited “comfort” effect. Additionally, further social network analysis shows that the bottleneck is mainly due to the consumers’ psychological gaps when comparing the usual e-commerce services, and cretailers can repair them through financial compensation and spiritual solace. The study explores e-commerce service quality at different pandemic stages with the help of text mining techniques, enriches the theory of e-commerce research, and alleviates the Hawthorne bias in traditional empirical studies. This study also provides a reference for e-retailers to improve service quality and respond to emergencies in a changing post-pandemic era.
Implementing Circular-Bioeconomy Principles across Two Value Chains of the Wood-Based Sector: A Conceptual Approach
Authors: Aggestam Filip , Giurca Alexandru
Published: November 2022

The wood-based sector has the potential to contribute significantly to the circular bioeconomy. This paper examines the potential for implementing circular bioeconomy principles across two value chains, covering five sectors: “sawnwood”, “bioenergy”, “construction”, “paper and pulp”, and “cellulose-based fibres and plastics”. The qualitative value chain analysis is limited to the material flow and demonstrates that a circular approach requires transformation across entire value chains. Implementing circular bioeconomy principles will require new business models, cooperation across sectors and companies, and the application of new technologies and management tools. More importantly, the results demonstrate that more applicable tools and methods are needed to analyse circularity. The results likewise confirm prevailing conceptual ambiguities surrounding the circular bioeconomy concept, such as the relationship between circularity and sustainability, which would need clarification. For example, circularity does not equal sustainability, nor guarantees economic viability for the wood-based sector. The paper argues that a circular wood-based system needs tailored governance approaches as there are no one-size-fits-all solutions. The wood-based sectors will also need to account for sustainability criteria and the natural limitations of wood (as a material) to close value retention loops.
Study of Disaster Susceptibility and Economic Vulnerability to Strengthen Disaster Risk Reduction Instruments in Batu City, Indonesia
Authors: Suprapto Firre An , Juanda Bambang , Rustiadi Ernan , Munibah Khursatul
Published: November 2022

Batu City in East Java has a thriving tourist area, which is not exempt from disaster susceptibility and economic vulnerability. These weaknesses have led to the strengthening of the disaster resilience system becoming a priority in terms of the Batu government’s disaster risk reduction. The main objective of this study is to improve disaster risk management through the reinforcement of the disaster risk reduction instrument, which can improve the alertness and the mitigation capability of DRR. This research analyzed the susceptibility levels of five disasters—flood, landslide, drought, land fire, and COVID-19—using a quantitative method with panel data and a survey questionnaire. The influence variable was disaster susceptibility, which quantified economic vulnerability through ArcGIS and ILWIS analysis to generate the disaster susceptibility rate. Economic vulnerability was analyzed using static panel data in STATA/R, which generated the economic vulnerability index. The results of this research indicate that there are five villages in the high level of vulnerability category, three villages in the moderate level of vulnerability category, and another sixteen villages/urban villages in the low level of vulnerability category. Furthermore, static panel analysis found that local economic vulnerability in Batu is significantly influenced by three of the five disasters discussed in this research.
Micrometeorological and Hydraulic Properties of an Urban Green Space on a Warm Summer Day in a Mediterranean City (Attica–Greece)
Authors: Proutsos Nikolaos D. , Solomou Alexandra D. , Petropoulou Michaela , Chatzipavlis Nikolaos E.
Published: November 2022

Urban Green Spaces (UGSs) are considered the most effective tool to mitigate Urban Heat Islands (UHIs). The optical properties of the materials and the vegetation types of the UGSs affect their surface temperatures, directly influencing their cooling ability. The hydraulic properties of urban soils are also affected by the vegetation coverage. The aim of this study is to investigate the temperature and reflected radiation (albedo) differences between UGS’s elements, around noon on a warm summer day, in Greece. The results indicate that green elements have smaller surface temperatures and higher reflectance compared to the artificial or the dry bare soil, presenting differences with the direct air temperature (measured above the surfaces with unshielded thermometers) −5.5 °C (shrubs), −3.8 °C (grass), +7.8 °C or +8.7 °C (paved surfaces inside or outside the UGS), +10.8 °C (dry bare soil), +12.2 °C (concrete) and +12.5 °C (asphalt), and albedo values 0.14 (grass and shrubs), 0.15 (dry bare soil), 0.27 (concrete), 0.21 (asphalt) and 0.20 (paved surfaces). The tree shades also produce great surface differences. The unsaturated hydraulic conductivity of the urban soil is greater than the surfaces covered with grass compared to the shrub-covered or bare soil, presenting values of 27.6, 10.8 and 11.4 mm h−1, respectively.
Refractive Changes after Glaucoma Surgery—A Comparison between Trabeculectomy and XEN Microstent Implantation
Authors: Bormann Caroline , Busch Catharina , Rehak Matus , Schmidt Manuela , Scharenberg Christian , Ziemssen Focke , Unterlauft Jan Darius
Published: November 2022

Best-corrected visual acuity often decreases temporarily or permanently after trabeculectomy (TE). The purpose of this study was to compare visual acuity and refractive changes after TE or XEN microstent implantation (XEN) in primary open-angle glaucoma (POAG) or pseudoexfoliation glaucoma (PEX) cases naïve to prior glaucoma surgery over a 24-month follow-up period. We analyzed 149 consecutive glaucoma patients who received either TE or XEN because of medically uncontrollable POAG or PEX. Intraocular pressure (IOP), IOP-lowering medication use, subjective and objective refraction and best-corrected visual acuity were evaluated. In addition, surgically induced astigmatism (SIA) was calculated and compared using the vector analysis method described by Jaffe and Clayman. A total of 93 eyes (85 POAG; 8 PEX) were treated with TE and 56 eyes (50 POAG; 6 PEX) with XEN. After 24 months, the mean IOP and number of IOP-lowering medications used decreased significantly after TE (p < 0.01) and XEN (p < 0.01). In the TE group, mean best-corrected visual acuity (BCVA) changed from 0.16 ± 0.26 to 0.23 ± 0.28 logMAR (p < 0.01) after 24 months, while mean BCVA did not change significantly in the XEN group (preoperative: 0.40 ± 0.50 logMAR, postoperative: 0.36 ± 0.49 logMAR; p = 0.28). SIA was almost the same in both groups at the end of the 24-month follow-up period (0.75 ± 0.60 diopters after TE and 0.81 ± 0.56 diopters after XEN; p = 0.57). In addition, there was no significant correlation between SIA and the observed BCVA changes or SIA and IOP reduction 12 or 24 months after TE or XEN. Our results demonstrate that TE and XEN are effective methods for reducing IOP and IOP-lowering medication use. The SIA was nearly similar in both groups. The SIA does not seem responsible for the decreased visual acuity after TE.
Accuracy of Seismic Response Evaluation of Two-Dimensional Analysis Model with Rigid Joints for RC Frame Buildings
Authors: Kang Jae-Do , Nagae Takuya , Jeong Seong-Hoon , Kajiwara Koichi
Published: November 2022

Three- or two-dimensional (2D) numerical models are used for the evaluation of the seismic performance of reinforced concrete (RC) buildings. This study examines a 2D numerical model for a specimen used in a full-scale four-story RC shaking-table test and evaluates the accuracy of the seismic response of the 2D numerical model, which is composed of a square fiber section model for the columns, a T-shape fiber section model for the beam and slab, and a rigid joint model for the beam–column joint. A parametric analysis of the effective slab width is performed to analyze its effects on the modal shape and natural period. The results suggest that the primary natural period of the considered model is almost identical to that associated with the experimental results. The applicability of the 2D numerical model for estimating the seismic response of the structure is established. By comparing the results of the seismic analysis and the experiment in the 50% amplitude of the JMA-Kobe wave, which corresponds to slightly exceeding VII on the modified Mercalli intensity scale, the root-mean-square percentage error of the 2D numerical model is 1.03% for the floor acceleration and 4.7% for the inter-story drift. Thus, the analytical model used in this study has sufficient accuracy in evaluating the seismic performance of buildings constructed in regions with a maximum seismic intensity of VII.
Probability Distribution Characteristics of Horizontal and Vertical Mechanical Properties of Rubber Bearings
Authors: Wu Di , Li Caiming , Yang Zhenyu , Liu Yang , Xiong Yan , Jiang Guoping
Published: November 2022

Rubber bearings are widely used to protect civil structures from destructive earthquakes. The mechanical properties of the bearings are the key technical parameters that determine the seismic isolation performance of isolated structures. To estimate the probability distribution of the mechanical properties related to rubber bearings (including horizontal stiffness, vertical stiffness, post-yield stiffness and yield force) under seismic events. Typical natural rubber bearings (NRBs) and lead-core rubber bearings (LRBs) were designed and fabricated, and the bearings were subjected to repeated load tests using a compression-shear testing machine. The test results of the horizontal and vertical mechanical properties of the bearings in the tests were basically consistent with the design values, and the rubber bearings showed stable mechanical behavior under repeated cyclic loading. The statistical analysis of the test results revealed that the relevant mechanical properties of the NRB and LRB specimens followed a lognormal or general extreme distribution with coefficients of variation mainly ranging from 0.86% to 5.6%. The dispersion of the yield force of LRB was the largest in the repeated tests of many mechanical parameters of typical rubber bearings.
Impact of Polypropylene Fibers on the Mechanical and Durability Characteristics of Rubber Tire Fine Aggregate Concrete
Authors: Karimi Pour Arash , Mohajeri Zahra , Noroozinejad Farsangi Ehsan
Published: November 2022

In this research, the consequence of using rubber tire aggregates (RTA) on the durability and mechanical characteristics of polypropylene fibers (PF) reinforced concrete is evaluated. Fifteen concrete mixtures were produced and tested in the laboratory. RTA was utilized instead of fine natural aggregates (FNA) to the concrete at concentrations of 0%, 5%, 10%, 15%, and 20% by a volumetric fraction; also, the contents of PF in the concrete mixtures were 0%, 1%, and 2% by weight fraction. Finally, the following parameters were tested for all the mixtures: compressive and tensile resistances, fracture, changes in drying shrinkage, bulk electrical resistivity, elastic moduli, and resonance occurrences. The control sample was the one without RTA and PF. According to the results, by adding RTA to the mixtures, the shrinkage deformation amplified, but the PF addition caused a decrease in the shrinkage deformation. Furthermore, adding 0%, 5%, 10%, and 15% RTA, with 2% PF leads to an upsurge in the flexural resistance by 34%, 24%, 16%, and 6%, respectively, relative to the control sample without PF and RTA. Moreover, the fracture energy of mixtures increased by utilizing PF and RTA simultaneously.
Syntheses of APTMS-Coated ZnO: An Investigation towards Penconazole Detection
Authors: Bauer Elvira Maria , Bogliardi Gabriele , Ricci Cosimo , Cecchetti Daniele , De Caro Tilde , Sennato Simona , Nucara Alessandro , Carbone Marilena
Published: November 2022

Extrinsic chemiluminescence can be an efficient tool for determining pesticides and fungicides, which do not possess any intrinsic fluorescent signal. On this basis, (3-aminopropyl) trimethoxysilane (APTMS)-coated ZnO (APTMS@ZnO) was synthesized and tested as an extrinsic probe for the fungicide penconazole. Several synthetic routes were probed using either a one-pot or two-steps method, in order to ensure both a green synthetic pathway and a good signal variation for the penconazole concentration. The synthesized samples were characterized using X-ray diffraction (XRD), infrared (IR), Raman and ultraviolet-visible (UV-Vis) spectroscopy, scanning electron microscopy (SEM) imaging and associated energy-dispersive X-ray (EDX) analysis. The average size of the synthesized ZnO nanoparticles (NPs) is 54 ± 10 nm, in line with previous preparations. Of all the samples, those synthesized in two steps, at temperatures ranging from room temperature (RT) to a maximum of 40 °C, using water solvent (G-APTMG@ZnO), appeared to be composed of nanoparticles, homogeneously coated with APTMS. Chemiluminescence tests of G-APTMG@ZnO, in the penconazole concentration range 0.7–1.7 ppm resulted in a quenching of the native signal between 6% and 19% with a good linear response, thus indicating a green pathway for detecting the contaminant. The estimated detection limit (LOD) is 0.1 ± 0.01 ppm.
Peculiarities of γ-Al2O3 Crystallization on the Surface of h-BN Particles
Authors: Grigoriev Sergey N. , Trusova Elena A. , Afzal Asya M. , Soe Thet Naing , Kurmysheva Alexandra Yu. , Kuznetsova Ekaterina , Smirnov Anton , Solís Pinargote Nestor Washington
Published: November 2022

The main goal of the present work was to synthesize a composite consisting of h-BN particles coated with a γ-Al2O3 nanolayer. A method was proposed for applying nanocrystalline γ-Al2O3 to h-BN particles using a sol–gel technique, which ensures the chemical homogeneity of the composite at the nano level. It has been determined that during crystallization on the h-BN surface, the proportion of spinel in alumina decreases from 40 wt.% in pure γ-Al2O3 to 30 wt.% as a result of the involvement of the B3+ ions from the surface nitride monolayers into the transition complex. For comparison, nano-alumina was synthesized from the same sol under the same conditions as the composite. The characterization of the obtained nanostructured powders was carried out using TEM and XRD. A mechanism is proposed for the formation of a nanostructured γ-Al2O3@h-BN composite during the interaction of Al-containing sol and h-BN suspension in aqueous organic media. The resulting composite is a promising model of powdered raw materials for the development of fine-grained ceramic materials for a wide range of applications.
Multi-Rate Parallel Real-Time Simulation Method for Doubly Fed Wind Power Systems Based on FPGA–CPU
Authors: Yang Guangrao , Li Yahui , Hao Zhenghang , Chen Zhuo , He Puxiang , Zhang Jing
Published: November 2022

A multi-rate parallel real-time simulation method based on FPGA–CPU is studied to realize the asynchronous co-simulation of the converter of doubly fed wind power systems with the wind turbine and external power grid. The doubly fed wind power system is partitioned by simulation step length, and the partitioned small-step-length data are processed using integral homogenization. For large-step data, an improved delay-compensated linear interpolation method combined with Newton interpolation is proposed for processing. The general small time-step (GST) model method is used to implement the FPGA modeling of the small-step converter, and resource optimization is achieved through timing time-division multiplexing. Asynchronous parallel co-simulation of a doubly fed wind power system is implemented on an FPGA–CPU co-simulation platform. Among them, the FPGA realizes the development of the converter HDL with a small step of 1 μs, while the CPU completes the simulation of the wind turbine and power grid synchronously with a large step of 50 μs. Finally, by comparing with MATLAB/Simulink offline simulation and analyzing the error, it is concluded that the simulation accuracy of the improved method in this paper is higher than that of the un-interpolated parallel simulation, which verifies the real-time performance and accuracy of the modeling and improved method in this paper.
A Subway Sliding Plug Door System Health State Adaptive Assessment Method Based on Interval Intelligent Recognition of Rotational Speed Operation Data Curve
Authors: Qi Hui , Chen Gaige , Ma Hongbo , Wang Xianzhi , Yang Yudong
Published: November 2022

The subway sliding plug door system is crucial for ensuring normal operation. Due to the differences in the structure and motor control procedures of different sliding plug door systems, the rotational speed monitoring data curves show great differences. It is a challenging problem to recognize the intervals of complex data curves, which fundamentally affect the sensitivity of feature extraction and the prediction of an assessment model. Aiming at the problem, a subway sliding plug door system health state adaptive assessment method is proposed based on interval intelligent recognition of rotational speed operation data curve. In the proposed method, firstly, the rotational speed operation data curve is adaptively divided by a long short-term memory (LSTM) neural network into four intervals, according to the motion characteristics of the door system. Secondly, the sensitive features of the door system are screened out by the random forest (RF) algorithm. Finally, the health state of the door system is assessed using the adaptive boosting (AdaBoost) classifier. The proposed method is comprehensively verified by the benchmark experiment data set. The results show that the average diagnostic accuracy of the method on multiple bench doors can reach 98.15%. The wider application scope and the higher state classification accuracy indicate that the proposed method has important engineering value and theoretical significance for the health management of subway sliding plug door systems.
Wolbachia Invasion Dynamics by Integrodifference Equations
Authors: Li Yijie , Guo Zhiming
Published: November 2022

Releasing mosquitoes infected with the endosymbiotic bacterium Wolbachia to invade and replace the wild populations can effectively interrupt dengue transmission. Recently, a reasonable discrete competitive non-spatial model was developed and the conditions for the successful invasion of Wolbachia were given. However, Wolbachia propagation is a matter of spatial dynamics. In this paper, we introduce a dispersal kernel and establish integrodifference equations, a class of discrete-time spatial diffusion systems that have recently gained much attention as an important tool for spatial ecology. We analyzed the spatial model by average dispersal success approximation to find the criteria for the successful spread of Wolbachia, and then compared it with the non-spatial model to discuss the effect of spatial parameters.
Microstructure and Properties of Multilayer Niobium-Aluminum Composites Fabricated by Explosive Welding
Authors: Malyutina Yulia N. , Anisimov Alexander G. , Popelyukh Albert I. , Lozhkin Vasiliy S. , Bataev Anatoly A. , Bataev Ivan A. , Lukyanov Yaroslav L. , Pai Vladimir V.
Published: November 2022

In this study, a layered composite material consisting of alternating aluminum and niobium layers and cladded on both sides with titanium plates was obtained by explosive welding. Microstructure of the composite was thoroughly studied using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), as well as by energy dispersive X-ray spectroscopy (EDX) and electron backscattered diffraction (EBSD). Microhardness measurements, tensile test, and impact strength test were carried out to evaluate the mechanical properties of the composite. Formation of mixing zones observed near all interfaces was explained by local melting and subsequent rapid solidification. Mixing zones at Nb/Al interfaces consisted of metastable amorphous and ultrafine crystalline phases, as well as NbAl3 and Nb2Al equilibrium phases. Niobium grains near the interface were significantly elongated, while aluminum grains were almost equiaxed. Crystalline grains inside the mixing zones did not have a distinct crystallographic texture. Microhardness of Al/Nb mixing zones was in the range 546–668 HV, which significantly exceeds the microhardness of initial materials. Tensile strength and impact strength of the composite were 535 MPa and 82 J/cm2, respectively. These results confirm the high bonding strength between the layers.
Multi-Band Power Amplifier Module with Back-Off Efficiency Improvement using Ultra-Compact 3D Vertical Stack Multi-Chip Package for Cellular Handsets
Authors: Zhang Zhihao , Li Jing , Peng Lin , Sun Bo
Published: November 2022

A highly integrated multi-mode multi-band (MMMB) power amplifier module (PAM) using hybrid bulk complementary metal oxide semiconductor (CMOS), gallium arsenide (GaAs) heterojunction bipolar transistor (HBT), and silicon-on-insulator (SOI) technologies for low band (LB, 824–915 MHz) and high band (HB, 1710–1980 MHz) is proposed. The hybrid MMMB PAM integrates a bulk CMOS controller die, a GaAs HBT power amplifier (PA) die and a SOI switch die on a six-layer laminate. To simultaneously obtain both highly efficient and highly linear characteristics over a wide range of input power levels, a parallel dual-chain PA strategy has been adopted to provide vary bias current and gain for low-power mode (LPM) and high-power mode (HPM) operation. Additionally, a broadband two-section low-pass output matching network design based on the suppression of high-order harmonics is proposed for enhanced efficiency and linearity. In order to achieve further miniaturization, a three-dimensional (3D) die stack multi-chip module (MCM) packaging structure, where the presented CMOS controller die is stacked vertically on the GaAs HBT PA die, is implemented. The measurement results show that the fabricated MMMB PAM achieves 26.1–27 dB of power gains and 38–38.4% of PAEs at an output power (Pout) of 28 dBm in the HPM, and 20.4–20.9 dB of power gains and 12.4–13.8% of PAEs at Pout of 17 dBm in the LPM over LB. For HB, power gains of 24.3–26.7 dB while maintaining PAEs of 38.2–39.9% at Pout of 28 dBm, and power gains of 15.9–17.5 dB while maintaining PAEs of 12.3–12.8% at Pout of 17 dBm are realized in the HPM and LPM, respectively. The fabricated PAM covering five frequency bands and operating at two power modes only occupies a 5 × 3.5 mm2 area. To the best of the authors’ knowledge, this work is the first demonstration of a MMMB PAM adopting an ultra-compact 3D vertical stack MCM package with favorable RF performance.
Identification of Leishmania infantum Epidemiology, Drug Resistance and Pathogenicity Biomarkers with Nanopore Sequencing
Authors: Martí-Carreras Joan , Carrasco Marina , Gómez-Ponce Marcel , Noguera-Julián Marc , Fisa Roser , Riera Cristina , Alcover Maria Magdalena , Roura Xavier , Ferrer Lluís , Francino Olga
Published: November 2022

The emergence of drug-resistant strains of the parasite Leishmania infantum infecting dogs and humans represents an increasing threat. L. infantum genomes are complex and unstable with extensive structural variations, ranging from aneuploidies to multiple copy number variations (CNVs). These CNVs have recently been validated as biomarkers of Leishmania concerning virulence, tissue tropism, and drug resistance. As a proof-of-concept to develop a novel diagnosis platform (LeishGenApp), four L. infantum samples from humans and dogs were nanopore sequenced. Samples were epidemiologically typed within the Mediterranean L. infantum group, identifying members of the JCP5 and non-JCP5 subgroups, using the conserved region (CR) of the maxicircle kinetoplast. Aneuploidies were frequent and heterogenous between samples, yet only chromosome 31 tetrasomy was common between all the samples. A high frequency of aneuploidies was observed for samples with long passage history (MHOM/TN/80/IPT-1), whereas fewer were detected for samples maintained in vivo (MCRI/ES/2006/CATB033). Twenty-two genes were studied to generate a genetic pharmacoresistance profile against miltefosine, allopurinol, trivalent antimonials, amphotericin, and paromomycin. MHOM/TN/80/IPT-1 and MCRI/ES/2006/CATB033 displayed a genetic profile with potential resistance against miltefosine and allopurinol. Meanwhile, MHOM/ES/2016/CATB101 and LCAN/ES/2020/CATB102 were identified as potentially resistant against paromomycin. All four samples displayed a genetic profile for resistance against trivalent antimonials. Overall, this proof-of-concept revealed the potential of nanopore sequencing and LeishGenApp for the determination of epidemiological, drug resistance, and pathogenicity biomarkers in L. infantum.
Prevalence, Subtype Distribution and Zoonotic Significance of Blastocystis sp. Isolates from Poultry, Cattle and Pets in Northern Egypt
Authors: Naguib Doaa , Gantois Nausicaa , Desramaut Jeremy , Arafat Nagah , Even Gaël , Certad Gabriela , Chabé Magali , Viscogliosi Eric
Published: November 2022

Blastocystis sp. is a widespread enteric protozoan that frequently infects human and animal groups. Despite its burden and zoonotic potential worldwide, epidemiological investigations remain limited in animal groups that come in contact with humans. Therefore, the largest survey ever conducted in North Africa was performed in Egypt with the aim to investigate the prevalence and subtype (ST) distribution of Blastocystis sp. in animals. For this purpose, a total of 889 fecal specimens were collected from chickens (217), cattle (373), dogs (144) and cats (155) from six governorates of northern Egypt. These specimens were then screened for the presence of Blastocystis sp. using a quantitative real-time PCR, followed by subtyping the isolates. The overall prevalence of Blastocystis sp. reached 9.2% (82/889), with the highest infection rates reported in chickens (17.0%) and domestic cattle (11.0%), highlighting an active circulation of the parasite in both animal groups. In contrast, the low prevalence in cats (2.6%) and the absence of the parasite in dogs suggested that pets are not natural hosts of Blastocystis sp. ST10 and ST14 were largely predominant in cattle, confirming that both STs represented cattle-adapted STs. The report of one ST3 and one ST4 isolate in this animal group could be explained by an accidental zoonosis from humans to animals. All but one of the subtyped isolates in poultry belonged to ST7, which was considered as an avian ST. The presence of a remaining isolate of ST14 likely reflected a transient infection from contact between birds and cattle feces. The same environmental contamination was also likely the source of the ST14 infection in three of the four positive cats, with the remaining animals infected by ST3 as the result of human-to-animal transmission. These occurrences and subtyping data, combined with those previously collected in the Egyptian population, implies that poultry could play a significant role as reservoir for zoonotic transmission, which would not be the case for cattle and pets.
Characterization of Coxiella burnetii Dugway Strain Host-Pathogen Interactions In Vivo
Authors: Tesfamariam Mahelat , Binette Picabo , Cockrell Diane , Beare Paul A. , Heinzen Robert A. , Shaia Carl , Long Carrie Mae
Published: November 2022

Coxiella burnetii is a Gram-negative, intracellular bacterium that causes the zoonosis Q fever. Among the many natural isolates of C. burnetii recovered from various sources, the Dugway group exhibits unique genetic characteristics, including the largest C. burnetii genomes. These strains were isolated during 1954–1958 from wild rodents from the Utah, USA desert. Despite retaining phase I lipopolysaccharide and the type 4B secretion system, two critical virulence factors, avirulence has been reported in a guinea pig infection model. Using guinea pig models, we evaluated the virulence, whole-cell vaccine (WCV) efficacy, and post-vaccination hypersensitivity (PVH) potential of a representative Dugway strain. Consistent with prior reports, Dugway appeared to be highly attenuated compared to a virulent strain. Indeed, Dugway-infected animals showed similarly low levels of fever, body weight loss, and splenomegaly like Nine Mile II-infected animals. When compared to a human Q fever vaccine, QVax®, Dugway WCV exhibited analogous protection against a heterologous Nine Mile I challenge. PVH was investigated in a skin-testing model which revealed significantly decreased maximum erythema in Dugway Δdot/icm WCV-skin-tested animals compared to that of QVax®. These data provide insight into this unique bacterial strain and implicate its potential use as a mutated WCV candidate.
Seroprevalence and Risk Factors Associated with Leishmania Infection in Dogs from Portugal
Authors: Almeida Maria , Maia Carla , Cristóvão José M. , Morgado Cátia , Barbosa Inês , Ibars Ruben Foj , Campino Lenea , Gonçalves Luzia , Cortes Sofia
Published: November 2022

Canine leishmaniosis (CanL) caused by Leishmania infantum is an important zoonosis in southwestern European countries where this disease is endemic, and dogs, as domestic animals in close contact with humans, are the reservoir hosts for the parasite. In Portugal, CanL is of relevant veterinary concern. The previous national study revealed an overall seroprevalence of 6.3%. Since then, new prophylactic measures, such as vaccines, have been introduced in Europe. The aim of this study was to update seroprevalence for Leishmania infection and reassess risk factors in Portugal. A cross-sectional study was conducted from January–March 2021 with 1860 client-owned dogs from continental Portugal. A questionnaire and whole blood samples on filter paper were collected and a direct agglutination test was used to calculate anti-Leishmania antibody titres. True seroprevalence was 12.5% (95% CI 10.3–13.2%). Potential risk factors associated with L. infantum infection in dogs were age ≥ 2 years (aOR = 1.68, 95% CI 1.1–2.6) and residing in the interior regions of the country (aOR = 1.92, 95% CI 1.3–2.9) and non-use of repellents (aOR = 1.75, 95% CI 1.2–2.5). The key to controlling CanL and its impact on Public Health in endemic areas lies in continuous implementation of prophylactic measures, through the correct use of repellents/insecticides and vaccines and early detection and monitoring of infected dogs.
Modulation in Techno-Functional, Textural Properties, In Vitro Starch Digestibility and Macromolecular–Structural Interactions of Pasta with Potato (Solanum tuberosum L.)
Authors: Sharma Savita , Malhotra Nancy , Singh Arashdeep , Sharma Rajan , Domínguez Rubén , Lorenzo José Manuel
Published: November 2022

The replacement of semolina with potato flour (PF) and potato mash (PM) at different levels was assessed for its effects on pasta quality. The results showed that the addition of PF and PM increased the pasting viscosity of the blends; in addition, PF enhanced the functional properties, while PM reduced them. The minimum cooking time decreased with PF and PM, while the PF pasta exhibited a higher cooking loss (5.02 to 10.44%) than the PM pasta, which exhibited a lower cooking loss. The pasta with PF and PM showed an increase in the total phenolic and flavonoid content, with reduced in vitro digestibility as confirmed by Fourier transform infrared spectroscopy. The PF pasta exhibited lower lightness and higher yellowness than the PM pasta, and its firmness and toughness also modulated owing to the complex interaction between potato starches and the gluten protein matrix, as evident from scanning electron microscopy. Sensory data revealed that pasta containing 30% PF and 16% PM was highly acceptable.
Structure Determination of Binuclear Triple-Decker Phthalocyaninato Complexes by NMR via Paramagnetic Shifts Analysis Using Symmetry Peculiarities
Authors: Babailov Sergey P. , Zapolotsky Eugeny N. , Fomin Eduard S. , Polovkova Marina A. , Kirakosyan Gayane A. , Martynov Alexander G. , Gorbunova Yulia G.
Published: November 2022

The detailed knowledge about the structure of multinuclear paramagnetic lanthanide complexes for the targeted design of these compounds with special magnetic, sensory, optical and electronic properties is a very important task. At the same time, establishing the structure of such multinuclear paramagnetic lanthanide complexes in solution, using NMR is a difficult task, since several paramagnetic centers act simultaneously on the resulting chemical shift of a particular nucleus. In this paper, we have demonstrated the possibility of molecular structure determination in solution on the example of binuclear triple-decker lanthanide(III) complexes with tetra-15-crown-5-phthalocyanine Ln2[(15C5)4Pc]3 {where Ln = Tb (1) and Dy (2)} by quantitative analysis of the pseudo-contact lanthanide-induced shifts (LIS). The symmetry of complexes was used for the simplification of the calculation of pseudo-contact shifts on the base of the expression for the magnetic susceptibility tensor in the arbitrary oriented magnetic axis system. Good agreement between the calculated and experimental shifts in the 1H NMR spectra indicates the similarity of the structure for the complexes 1 and 2 in solution of CDCl3 and the structure in the crystalline phase, found from the data of the X-ray structural study of the similar complex Lu2[(15C5)4Pc]3. The described approach can be useful for LIS analysis of other polynuclear symmetric lanthanide complexes.
Intramolecular Folding of PolyT Oligonucleotides Induced by Cooperative Binding of Silver(I) Ions
Authors: Hao Jinghua , Cao Dong , Zhao Qiang , Zhang Dapeng , Wang Hailin
Published: November 2022

Ag+-bridged T-Ag+-T was recently discovered in a Ag+-DNA nanowire crystal, but it was reported that Ag+ had little to no affinity to T nucleobases and T-rich oligonucleotides in solution. Therefore, the binding mode for the formation of this type of novel metallo base pair in solution is elusive. Herein, we demonstrate that Ag+ can interact with polyT oligonucleotides once the concentration of Ag+ in solution exceeds a threshold value. The threshold value is independent of the concentration of the polyT oligonucleotide but is inversely proportional to the length of the polyT oligonucleotide. The polyT oligonucleotides are intramolecularly folded due to their positively cooperative formation and the stack of T-Ag+-T base pairs, resulting in the 5′- and 3′-ends being in close proximity to each other. The intramolecular Ag+-folded polyT oligonucleotide has a higher thermal stability than the duplex and can be reversibly modulated by cysteine.
Comparative Volatilomic Profile of Three Finger Lime (Citrus australasica) Cultivars Based on Chemometrics Analysis of HS-SPME/GC–MS Data
Authors: Cozzolino Rosaria , Câmara José S. , Malorni Livia , Amato Giuseppe , Cannavacciuolo Ciro , Masullo Milena , Piacente Sonia
Published: November 2022

Finger lime is receiving growing attention as an ingredient of gastronomic preparations of haute cuisine for its delicious flavor and fragrance and for its appealing aspect. Volatile compounds play a crucial role in determining the organoleptic characteristics of the fruit and its pleasantness for consumers. The aim of the present study was to investigate the volatile profiles by headspace solid phase micro-extraction (HS-SPME) coupled to gas chromatography–mass spectrometry (GC–MS) in the peel and, for the first time, in the pulp of three Australian finger lime cultivars grown in Sicily (southern Italy): Pink Pearl, Sanguinea, and Faustrime, allowing to overall identify 84 volatile organic compounds (VOCs). The analytical data showed that the three cultivars were characterized by distinct volatile chemotypes: limonene/sabinene/bicyclogermacrene in the Pink Pearl, limonene/γ-terpinene/bicyclogermacrene in the Sanguinea, and limonene/β-phellandrene/γ-terpinene in the Faustrime. Moreover, some volatiles, found exclusively in one cultivar, could be considered potential markers of the individual cultivar. PCA allowed us to clearly discriminate the samples into three clusters: the first related to the Sanguinea peel, the second to the Faustrime peel, and a third group associated with the Pink Pearl peel along with the pulp of the three cultivars. Accordingly, the VOCs that mostly contributed to the differentiation of the three finger lime cultivars were also identified. Among them, D-limonene, sabinene γ-terpinene, α-pinene, α-phellandrene, β-myrcene, p-cymene, linalool, δ-elemene, ledene, bicyclogermacrene β-citronellol, α-bergamotene, α-caryophillene, and β-bisabolene, have been previously reported to exhibit important biological activities, suggesting that these cultivars, in addition to possessing unique volatile profiles, can show promise for several applications in pharmaceutical and food industry, namely for development of functional foods.
Deuterium Distribution in Fe/V Multi-Layered Films
Authors: Gemma Ryota , Al-Kassab Talaat , Pundt Astrid
Published: November 2022

The recent progress of Atom Probe Tomography (APT) has opened up atomic-scale elemental analysis including hydrogen species. For APT measurements, the use of deuterium is highly recommended, due to its low mobility compared to the fast and quantum mechanically tunneling isotope hydrogen. In addition, deuterium can be distinguished from hydrogen originating from the APT analysis chamber. To date, however, APT studies on materials with high D concentrations are scarce. In this study, the D concentration profile in a Fe/V multi-layered film sample was investigated, and spanned a wide concentration range. The mean hydrogen isotope concentration was alternatively quantified by electromotive force (EMF) measurements on a similar Fe/V film, thus verifying the APT results. The reduction found in the D concentration at the Fe/V interface results from local alloying at the Fe/V interfaces which accompanies a change in the available volume in the V lattice. Even at the same Fe concentration, the shape of the observed D depth profile was asymmetric at high D2 pressures. This indicates a stress impact caused by the deposition sequence.
Analyses of Fatty Acids, Proteins, Ascorbic Acid, Bioactive Phenolic Compounds and Antioxidant Activity of Canadian Barley Cultivars and Elite Germplasm
Authors: Wijekoon Champa , Netticadan Thomas , Sabra Ali , Yu Liping , Kodikara Chamali , Badea Ana
Published: November 2022

Barley (Hordeum vulgare L.) grain is rich in fiber and antioxidant phytochemicals, including fatty acids, proteins, phenolic compounds, vitamins, and minerals, that offer various health benefits. Research on identifying different barley genotypes based on their health attributes is very limited. In this study, we performed an analysis of fatty acids, proteins, ascorbic acid, phenolic compounds, and antioxidant activity of several Canadian barley cultivars and elite breeding lines. Linoleic acid (C18:2) was the predominant fatty acid present in the tested barley cultivars. The cultivar CDC Bold contained the highest amount of ascorbic acid, while the highest protein content was in CDC Mindon. An assessment of the free and bound phenolic compounds of barley grains showed quantitative changes among different genotypes of Canadian barley. Catechin is the most abundant molecule in free phenolics, while ferulic acid and para-coumeric acid are the most abundant in bound phenolics. Ferulic acid and vanillic acid were molecules detected in the soluble free fraction of all genotypes. Para-coumeric acid was detected only in genotypes such as CDC Copeland, CDC Bold, Lowe, and elite breeding Line 5 of both free and bound fractions of barley. Breeding Line 5 had the lowest antioxidant activity. An analysis of the above molecules and parameters of Canadian barley would help to uncover potential biomarkers in order to distinguish individual barley genotypes.
Quantitative Analysis of Solar Photovoltaic Panel Performance with Size-Varied Dust Pollutants Deposition Using Different Machine Learning Approaches
Authors: Tripathi Abhishek Kumar , Aruna Mangalpady , Venkatesan Elumalai Perumal , Abbas Mohamed , Afzal Asif , Shaik Saboor , Linul Emanoil
Published: November 2022

In this paper, the impact of dust deposition on solar photovoltaic (PV) panels was examined, using experimental and machine learning (ML) approaches for different sizes of dust pollutants. The experimental investigation was performed using five different sizes of dust pollutants with a deposition density of 33.48 g/m2 on the panel surface. It has been noted that the zero-resistance current of the PV panel is reduced by up to 49.01% due to the presence of small-size particles and 15.68% for large-size (ranging from 600 µ to 850 µ). In addition, a significant reduction of nearly 40% in sunlight penetration into the PV panel surface was observed due to the deposition of a smaller size of dust pollutants compared to the larger size. Subsequently, different ML regression models, namely support vector machine (SVMR), multiple linear (MLR) and Gaussian (GR), were considered and compared to predict the output power of solar PV panels under the varied size of dust deposition. The outcomes of the ML approach showed that the SVMR algorithms provide optimal performance with MAE, MSE and R2 values of 0.1589, 0.0328 and 0.9919, respectively; while GR had the worst performance. The predicted output power values are in good agreement with the experimental values, showing that the proposed ML approaches are suitable for predicting the output power in any harsh and dusty environment.