bluuebunny/crossref_metadata_2025 huggingface dataset:
Morphological and Phylogenetic Characterization of Three Novel Thaxterogaster (Cortinariaceae) Species from China with an Emphasis on Their Subtropical Distribution
Authors: Xie Meng-Le , Feng Na , Lin Wen-Fei , Su Wen-Ying , Li Yi , Yang Zhen-Quan
Published: October 2023

Three new phlegmaciod species of Thaxterogaster, T. borealicremeolinus, T. rufopurpureus, and T. sinopurpurascens spp. nov., from subtropical China were described based on their morphological characteristics and molecular data. Thaxterogaster borealicremeolinus belongs to the sect. Cremeolinae and differs from the other species in this section in its larger basidiospores and its habitat in the Northern Hemisphere associated with Quercus sp. trees. Thaxterogaster rufopurpureus and T. sinopurpurascens belong to sect. Purpurascentes, in which T. rufopurpureus is characterized by a pileus with a reddish-brown coloration when mature and a clavate stipe, while T. sinopurpurascens is characterized by a violet basidiomata, except for a greyish orange to brown pileus, the distinctly marginate bulb of its stipe, and its distribution in subtropical China. The phylogenetic analyses were performed based on nrITS, and detailed descriptions of the new species are provided herein.
Pandemics and Stock Price Volatility: A Sectoral Analysis
Authors: Koirala Niraj Prasad , Nyiwul Linus
Published: October 2023

In this paper, we assess the impacts of the five most recent pandemics on the volatility of stock prices across forty-nine sectors of the economy in the United States. These five most recent pandemics are the 1957–1958 Asian flu, the 1977 Russian flu, SARS-CoV-1, swine flu and COVID-19. Applying the GJR-GARCH model, we find that pandemics other than COVID-19 have heterogeneous impacts on the volatility of stock returns. The results of our analysis indicate that COVID-19 has increased the volatility of stock returns in all sectors. Similarly, stocks in more than seventy percent of sectors in our study declined during the ongoing pandemic, perhaps reflecting the severity of the pandemic. In addition, our results on sectors such as healthcare and natural gas diverge from other literature. The mixed results on SARS-CoV-1 are partially explained by the fact it emerged at a time when stock valuations were particularly pessimistic. In the case of Russian flu, it was relatively short-lived and limited in spread relative to other pandemics in our study.
Therapeutics for Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD)
Authors: Bhopale Kamlesh K. , Srinivasan Mukund P.
Published: October 2023

Metabolic dysfunction associated fatty liver disease (MAFLD) has been recently recognized as a new global chronic liver disease entity with non-alcoholic fatty liver disease (NAFLD) associated with overweight/obesity or type 2 diabetes mellitus (T2DM) and evidence of metabolic dysregulation. Due to the rising rates of obesity and diabetes, MAFLD is considered a rapidly emerging chronic liver disease globally. Nearly 25–30% of the global population poses health issues due to MAFLD with a substantial economic burden to societies. Disease progression depends on the persistence of risk factors and etiological agents, from simple steatosis, hepatitis, fibrosis, to cirrhosis, and if untreated, leads to hepatocellular carcinoma. In this review article we summarize various risk and etiological factors, diagnostic techniques, and therapeutic evaluation of pharmacological agents developed for MAFLD. Effective pharmaceutical agents for the treatment of MAFLD (and NAFLD) are lacking, and research is ongoing to search for effective medications in this direction. Currently, pioglitazone is advised for MAFLD patients, whereas Vitamin E is advised for non-diabetic MAFLD patients with ≥F2 non-cirrhosis. Current approaches to disease management emphasize diet control, lifestyle changes, and weight loss. In this review, we summarized the pharmacological agents currently being developed and their current status to treat patients with MAFLD.
Simulation of, Optimization of, and Experimentation with Small Heat Pipes Produced Using Selective Laser Melting Technology
Authors: Zhou Jianfeng , Teng Lai , Shen Yinyi , Jin Zhonghe
Published: October 2023

With the development of microsatellite technology, the heat generated by onboard components is increasing, leading to a growing demand for improved thermal dissipation in small satellites. Metal powder additive manufacturing technology offers the possibility of customizing and miniaturizing heat pipes to meet the specific requirements of small satellites. This article introduces a small-scale heat pipe designed using selective laser melting (SLM) technology. The heat pipe’s material, structure, and internal working fluid were determined based on mission requirements. Subsequently, the SolidWorks 2021 software was used for heat pipe modeling, and the ANSYS 2021R2 finite element analysis software was employed to simulate the heat transfer performance of the designed heat pipe, confirming its feasibility. The heat pipe’s structure was optimized using multi-objective regression analysis, considering various structural parameters, such as the channel diameter, vapor chamber height, and narrow gap width. The simulation results demonstrate that the optimized heat pipe achieved a 10.5% reduction in thermal resistance and an 11.6% increase in equivalent thermal conductivity compared to the original heat pipe. Furthermore, compared to conventional metal heat-conducting rods, the optimized heat pipe showed a 38.5% decrease in thermal resistance and a 62.19% increase in equivalent thermal conductivity. The heat pipe was then fabricated using a 3D printer (EOS M280), and a vacuum experimental system was established to investigate its heat transfer characteristics. The experimental results show that the heat pipe operated most efficiently at a heating power of 20 W, reached its maximum heat transfer capacity at 22 W, and had an optimal fill ratio of 30%. These results highlight the excellent performance of the heat pipe and the promising application prospects for SLM technology in the field of small satellites.
Degradation of Mechanical Properties of Graphene Oxide Concrete under Sulfate Attack and Freeze–Thaw Cycle Environment
Authors: Qian Ji , Zhou Lin-Qiang , Wang Xu , Yang Ji-Peng
Published: October 2023

In this paper, firstly, the effects of graphene oxide on the mechanical properties of concrete were investigated. Secondly, the degradation and mechanism of the mechanical properties of graphene oxide concrete (GOC) under sulfate attack and a freeze–thaw environment were investigated. In addition, the dynamic modulus of elasticity (MOEdy) and uniaxial compressive strength (UCS) of the GOC were measured under different environmental conditions. According to the test results, the incorporation of graphene oxide in appropriate admixtures could improve the mechanical properties of concrete in these two working environments. It is worth noting that this effect is most pronounced when 0.05 wt% graphene oxide is incorporated. In the sulfate attack environment, the MOEdy and UTS of the GOC0.05% specimen at 120 cycles decreased by 22.28% and 24.23%, respectively, compared with the normal concrete specimens. In the freeze–thaw environment, the MOEdy and UTS of the GOC0.05% specimen at 90 cycles decreased by 13.96% and 7.58%, respectively, compared with the normal concrete specimens. The scanning electron microscope (SEM) analysis showed that graphene oxide could adjust the aggregation state of cement hydration products and its own reaction with some cement hydration crystals to form strong covalent bonds, thereby improving and enhancing the microstructure density.
Improved Thermoelectric Performance of Sb2Te3 Nanosheets by Coating Pt Particles in Wide Medium-Temperature Zone
Authors: Guan Qing-Ling , Dong Li-Quan , Hao Qun
Published: October 2023

The p-type Sb2Te3 alloy, a binary compound belonging to the V2VI3-based materials, has been widely used as a commercial material in the room-temperature zone. However, its low thermoelectric performance hinders its application in the low-medium temperature range. In this study, we prepared Sb2Te3 nanosheets coated with nanometer-sized Pt particles using a combination of solvothermal and photo-reduction methods. Our findings demonstrate that despite the adverse effects on certain properties, the addition of Pt particles to Sb2Te3 significantly improves the thermoelectric properties, primarily due to the enhanced electronic conductivity. The optimal ZT value reached 1.67 at 573 K for Sb2Te3 coated with 0.2 wt% Pt particles, and it remained above 1.0 within the temperature range of 333–573 K. These values represent a 47% and 49% increase, respectively, compared to the pure Sb2Te3 matrix. This enhancement in thermoelectric performance can be attributed to the presence of Pt metal particles, which effectively enhance carrier and phonon transport properties. Additionally, we conducted a Density Functional Theory (DFT) study to gain further insights into the underlying mechanisms. The results revealed that Sb2Te3 doped with Pt exhibited a doping level in the band structure, and a sharp rise in the Density of States (DOS) was observed. This sharp rise can be attributed to the presence of Pt atoms, which lead to enhanced electronic conductivity. In conclusion, our findings demonstrate that the incorporation of nanometer-sized Pt particles effectively improves the carrier and phonon transport properties of the Sb2Te3 alloy. This makes it a promising candidate for medium-temperature thermoelectric applications, as evidenced by the significant enhancement in thermoelectric performance achieved in this study.
Torsional Behavior of Concrete-Filled Circular Steel Tubes Strengthened with CFRP
Authors: Qing-li Wang , Hui-ying Zhang , Peng Kuan
Published: October 2023

In order to study the torsional performance of steel tube concrete after reinforcement with a carbon-fiber-reinforced polymer (CFRP), the mechanical properties of 18 specimens were studied from both experimental and finite element perspectives. The T-θ curve and τ-γ curve of the specimen were measured in the experiment, and the failure mode of the specimen was analyzed. Subsequently, a reasonable finite element model was established using ABAQUS software, and the variation in various parameters surrounding the performance of the specimen was analyzed. Based on the experimental and finite element results, a formula for calculating the bearing capacity of the specimen was established. According to both the experimental and numerical results, the torsional bearing capacity of C-CF-CFRP-ST, defined as the torque endured by the specimen with maximum shear strain, was determined to be 15,000 με, together with its corresponding calculation formula. After the test, it was demonstrated that the main components of the concrete-filled CFRP-steel tube composite material—the steel tube and the concrete—could be used as reusable resources.
The Effect of Curing Conditions on the Service Life of 3D Printed Concrete Formwork
Authors: Bekaert Michiel , Van Tittelboom Kim , De Schutter Geert
Published: October 2023

Complex concrete elements are typically produced with lost formwork made out of timber or plastic. After usage, these timber or plastic panels are disposed of. This makes complex lost formwork a polluting and high-cost-inducing aspect of concrete construction. A possible solution for this problem could be 3D printing of concrete. This high degree of freedom construction process could easily be used to produce complex formwork. As the formwork stays in place, it has a function during and after the hardening of the inner concrete. Before hardening, the formwork keeps the fresh concrete in place. After hardening, the printed formwork takes the function of a concrete cover. The concrete cover protects the steel reinforcement against aggressive environmental substances such as chlorides and carbon dioxide. To properly execute this function, the printed material and the transition between printed material and inner concrete need to perform at least as well as the inner material. This experimental research investigates the usability of a 3D printed concrete mixture as a concrete cover in a combined concrete structure. The effect of the curing condition as well as two different surface finishing techniques of the printed formwork are taken into account. The effect of the different parameters is compared based on existing service life models. Results indicate that proper curing of the printed formwork is of key importance in order to obtain significant resistance against carbonation- and chloride-induced corrosion. Adjusting the nozzle with side trowels improves the resistance of the printed material against chloride intrusion and carbonation but has only a limited effect on the service life extension.
Power Generation Enhancement of Horizontal Axis Wind Turbines Using Bioinspired Airfoils: A CFD Study
Authors: Kaviani Hamid R. , Moshfeghi Mohammad
Published: October 2023

This research investigates the performance implications of employing a bioinspired airfoil (seagull’s wing cross-section) in horizontal-axis wind turbines. Specifically, we replaced the S809 airfoil from NREL Phase VI with an airfoil modeled after seagull wings. Initially, we calibrated four coefficients of the GEKO turbulence model for both the S809 and the bioinspired airfoil, utilizing experimental data. Subsequently, using the calibrated generalized k-ω (GEKO) model, we conducted a comparative analysis between the S809 and the seagull airfoils, revealing the considerable superiority of the seagull airfoil in terms of lift and drag coefficients. Furthermore, we numerically simulated the original NREL Phase VI turbine and a modified version where the S809 airfoil was replaced with the seagull airfoil using 3D computational fluid dynamics (CFD) with the airfoil-based-calibrated GEKO turbulence model. This investigation spanned a wide range of air speeds, including 7 m/s, 13 m/s, and 25 m/s. At these wind speeds, we observed a substantial increase in turbine power generation, with enhancements of 47.2%, 204.4%, and 103.9%, respectively. This study underscores the significant influence of nature’s designs in advancing energy extraction within industries, particularly within the wind energy sector.
Investigation of the Weak Solvability of One Viscoelastic Fractional Voigt Model
Authors: Zvyagin Andrey , Kostenko Ekaterina
Published: October 2023

This article is devoted to the investigation of the weak solvability to the initial boundary value problem, which describes the viscoelastic fluid motion with memory. The memory of the fluid is considered not at a constant position of the fluid particle (as in most papers on this topic), but along the trajectory of the fluid particle (which is more physical). This leads to the appearance of an unknown function z, which is the trajectory of fluid particles and is determined by the velocity v of a fluid particle. However, in this case, the velocity v belongs to L2(0,T;V1), which does not allow the use of the classical Cauchy Problem solution. Therefore, we use the theory of regular Lagrangian flows to correctly determine the trajectory of the particle. This paper establishes the existence of weak solutions to the considered problem. For this purpose, the topological approximation approach to the study of mathematical hydrodynamics problems, constructed by Prof. V. G. Zvyagin, is used.
Design and Analysis of Extended Exponentially Weighted Moving Average Signed-Rank Control Charts for Monitoring the Process Mean
Authors: Talordphop Khanittha , Areepong Yupaporn , Sukparungsee Saowanit
Published: October 2023

In the real world, a nonparametric control chart is a powerful substitute for enhancing outcome quality, although the fundamental procedure characteristic often fails to match the distribution assumptions. This study aims to construct and evaluate an extended exponentially weighted moving average control chart based on signed-rank statistics (EEWMA-SR) for recognizing changes in procedures. According to the study, the proposed chart proves more potent recognition of shifts in the process mean, predominantly small shifts, than the other control charts by the average run length in Monte Carlo simulation. Applying the proposed control chart to an actual dataset yielded events that corroborated the study discoveries.
Research on the Factors Influencing Tourism Revenue of Shandong Province in China Based on Uncertain Regression Analysis
Authors: Chen Shukun , Ning Yufu , Wang Lihui , Wang Shuai
Published: October 2023

According to the analysis of historical tourism data, it was found that tourism revenue is influenced by multiple factors, and there exists a linear relationship between these factors and tourism revenue. Therefore, this paper employs a linear regression model to investigate the factors influencing tourism revenue. However, research on tourism data has found that the disturbance term of the linear regression model is not frequency-stable. This indicates that the disturbance term should be an uncertain variable rather than a random variable. Therefore, this paper adopts an uncertain linear regression analysis model and employs the tourism data of Shandong Province in China from 2011 to 2020 as the sample to investigate the factors influencing tourism revenue. The study provides parameter estimation and residual analysis of the model, as well as predictions and confidence intervals of tourism revenue. Additionally, through an uncertain hypothesis test, it was verified that the adopted model fitted the relevant tourism data well. The results show that factors such as the number of travel agencies, railway length, domestic tourist numbers, and per capita disposable income of urban residents have a significant impact on tourism revenue. Based on the study, recommendations and measures for improving tourism revenue of Shandong Province are proposed.
Genome Sequencing-Based Mining and Characterization of a Novel Alginate Lyase from Vibrio alginolyticus S10 for Specific Production of Disaccharides
Authors: Shu Zhiqiang , Wang Gongming , Liu Fang , Xu Yingjiang , Sun Jianan , Hu Yang , Dong Hao , Zhang Jian
Published: October 2023

Alginate oligosaccharides prepared by alginate lyases attracted great attention because of their desirable biological activities. However, the hydrolysis products are always a mixture of oligosaccharides with different degrees of polymerization, which increases the production cost because of the following purification procedures. In this study, an alginate lyase, Alg4755, with high product specificity was identified, heterologously expressed, and characterized from Vibrio alginolyticus S10, which was isolated from the intestine of sea cucumber. Alg4755 belonged to the PL7 family with two catalytic domains, which was composed of 583 amino acids. Enzymatic characterization results show that the optimal reaction temperature and pH of Alg4755 were 35 °C and 8.0, respectively. Furthermore, Alg4755 was identified to have high thermal and pH stability. Moreover, the final hydrolysis products of sodium alginate catalyzed by Alg4755 were mainly alginate disaccharides with a small amount of alginate trisaccharides. The results demonstrate that alginate lyase Alg4755 could have a broad application prospect because of its high product specificity and desirable catalytic properties.
Natural Products and Pharmacological Properties of Symbiotic Bacillota (Firmicutes) of Marine Macroalgae
Authors: Chukwudulue Uche M. , Barger Natalia , Dubovis Michael , Luzzatto Knaan Tal
Published: October 2023

The shift from the terrestrial to the marine environment to discover natural products has given rise to novel bioactive compounds, some of which have been approved for human medicine. However, the ocean, which makes up nearly three-quarters of the Earth’s surface, contains macro- and microorganisms whose natural products are yet to be explored. Among these underexplored marine organisms are macroalgae and their symbiotic microbes, such as Bacillota, a phylum of mostly Gram-positive bacteria previously known as Firmicutes. Macroalgae-associated Bacillota often produce chemical compounds that protect them and their hosts from competitive and harmful rivals. Here, we summarised the natural products made by macroalgae-associated Bacillota and their pharmacological properties. We discovered that these Bacillota are efficient producers of novel biologically active molecules. However, only a few macroalgae had been investigated for chemical constituents of their Bacillota: nine brown, five red and one green algae. Thus, Bacillota, especially from the marine habitat, should be investigated for potential pharmaceutical leads. Moreover, additional diverse biological assays for the isolated molecules of macroalgae Bacillota should be implemented to expand their bioactivity profiles, as only antibacterial properties were tested for most compounds.
Polyunsaturated Aldehydes Profile in the Diatom Cyclotella cryptica Is Sensitive to Changes in Its Phycosphere Bacterial Assemblages
Authors: Hernanz-Torrijos María , Ortega María J. , Úbeda Bárbara , Bartual Ana
Published: October 2023

Diatoms are responsible for the fixation of ca. 20% of the global CO2 and live associated with bacteria that utilize the organic substances produced by them. Current research trends in marine microbial ecology show which diatom and bacteria interact mediated through the production and exchange of infochemicals. Polyunsaturated aldehydes (PUA) are organic molecules released by diatoms that are considered to have infochemical properties. In this work, we investigated the possible role of PUA as a mediator in diatom–bacteria interactions. To this end, we compare the PUA profile of a newly isolated oceanic PUA producer diatom, Cyclotella cryptica, co-cultured with and without associated bacteria at two phosphate availability conditions. We found that the PUA profile of C. cryptica cultured axenically was different than its profile when it was co-cultured with autochthonous (naturally associated) and non-autochthonous bacteria (unnaturally inoculated). We also observed that bacterial presence significantly enhanced diatom growth and that C. cryptica modulated the percentage of released PUA in response to the presence of bacteria, also depending on the consortium type. Based on our results, we propose that this diatom could use released PUA as a specific organic matter sign to attract beneficial bacteria for constructing its own phycosphere, for more beneficial growth.
Effects of Foods of Mesoamerican Origin in Adipose Tissue and Liver-Related Metabolism
Authors: Meza-Rios Alejandra , López-Villalobos Erika Fabiola , Anguiano-Sevilla Luis Alberto , Ruiz-Quezada Sandra Luz , Velazquez-Juarez Gilberto , López-Roa Rocío Ivette , Marin-Molina Ana Laura , Zepeda-Morales Adelaida Sara Minia
Published: October 2023

Adipose tissue and liver metabolism play a key role in maintaining body homeostasis; therefore, their impairment conduces a pathological state. Nowadays, occidental lifestyle is a common etiological issue among a variety of chronic diseases, while diet is a unique strategy to prevent obesity and liver metabolism impairment and is a powerful player in the treatment of metabolic-related diseases. Mesoamerican foods are rich in bioactive molecules that enhance and improve adipose tissue and liver performance and represent a prophylactic and therapeutic alternative for disorders related to the loss of homeostasis in the metabolism of these two important tissues.
Remimazolam Anaphylaxis during Induction of General Anesthesia Confirmed by Provocation Test—A Case Report and Literature Review
Authors: Lee Sangho , Park Joyoung , Kim Na Hei , Hong Halin , Sohn Kyoung Hee , Kang Hee Yong , Kim Mi Kyeong , You Ann Hee
Published: October 2023

Background: Remimazolam besylate, a newly developed drug, is linked to various anaphylaxis cases. We present a case of remimazolam anaphylaxis confirmed using a provocation test. Case: A 51-year-old female patient was scheduled for humeral pinning. General anesthesia was induced using remimazolam, rocuronium, and remifentanil. After tracheal intubation, the patient experienced decreased blood pressure, increased heart rate, and a systemic rash. Epinephrine was administered repeatedly, and the patient’s vital signs stabilized. Acute phase tryptase levels were within normal limits. After four weeks, intradermal test results were negative. When remimazolam was administered intravenously for the provocation test, facial swelling, flushing, and coughing occurred, which improved with epinephrine. The culprit drug was identified as remimazolam using a provocation test. Conclusions: When anaphylaxis occurs during anesthesia induction, remimazolam should not be ruled out as the causative drug. If the skin test result for remimazolam is negative, a provocation test should be considered. The provocation test should be initiated cautiously at a low dose under careful patient monitoring.
Hypoglycemic Effect of an Herbal Decoction (Modified Gangsimtang) in a Patient with Severe Type 2 Diabetes Mellitus Refusing Oral Anti-Diabetic Medication: A Case Report
Authors: Joo Sungjun , Chun Hyonjun , Lee Jisu , Seo Seungmin , Lee Jungmin , Leem Jungtae
Published: October 2023

There is growing interest in alternative therapies for type 2 diabetes mellitus (T2DM) because some patients refuse to receive conventional therapies. In East Asia, herbal medicines are often used to treat T2DM, and modified Gangsimtang (mGST) is prescribed to treat a condition called wasting thirst (消渴), which resembles T2DM. This study reported the treatment of hyperglycemia using herbal medicines without oral hypoglycemic agents or insulin therapy. Case presentation: A 36-year-old man with obesity was diagnosed with T2DM four years prior to hospitalization and experienced blood glucose level reduction from 22.2–27.8 mmol/L (400–500 mg/dL) to 5.6–11.1 mmol/L (100–200 mg/dL) by using herbal medicines. He visited D Korean Medicine Hospital with chronic polydipsia and general weakness as chief complaints. He was diagnosed with T2DM on the basis of a hemoglobin A1c level of 11.7% and 2 h postprandial blood glucose level of >25.0 mmol/L (450 mg/dL). Moreover, he was diagnosed with a “dual deficiency of qi and yin” (氣陰兩虛) because of ordinary symptoms (素證). During his 30-day inpatient treatment, the patient received mGST 120 mL thrice daily; as a result, his postprandial blood glucose level decreased from 25.3 mmol/L (455 mg/dL) to 8.6 mmol/L (154 mg/dL), polydipsia decreased (visual analog scale score decreased from six to one), and triglyceride levels decreased from 11.7 mmol/L (1031 mg/dL) to 2.0 mmol/L (174 mg/dL). Plasma glucose levels remained stable for 6 months after the treatment, and no adverse events were observed over 200 days. We administered an herbal decoction to decrease plasma glucose levels without using oral hypoglycemic agents or insulin. Conclusions: Herbal decoctions such as mGST can reduce hyperglycemia in patients with T2DM who refuse conventional therapy.
Antineutrophil Cytoplasmic Antibody-Associated Vasculitis and the Risk of Developing Incidental Tuberculosis: A Population-Based Cohort Study
Authors: Chan Shan-Ho , Li Ming-Feng , Ou Shih-Hsiang , Lin Mei-Chen , Wang Jen-Hung , Lee Po-Tsang , Chen Hsin-Yu
Published: October 2023

Background and Objectives: Treatment for antineutrophil cytoplasmic antibody-associated vasculitis (AAV) must deal with immunosuppression, as well as infections associated with a compromised immune system, such as tuberculosis (TB). Our aim was to examine the risk of incidental TB after diagnosis of AAV. Materials and Methods: This retrospective population-based cohort study was based on the data from the National Health Insurance Research Database in Taiwan. Patients with newly diagnosed granulomatous polyangiitis or microscopic polyangiitis were identified between 1 January 2000 and 31 December 2012. The primary outcome was risk of incidental TB. Cox proportional hazard models were used to evaluate the association between AAV and incidental TB. Results: A total of 2257 patients with AAV and a propensity-score matched cohort of 9028 patients were studied. Overall, patients with AAV were at a 1.48× higher risk of contracting incidental TB than the patients in the matched cohort (adjusted HR 1.48; 95% confidence interval [CI], 1.02–2.15). Note that the highest risk of contracting incidental TB was in the first two years following a diagnosis of AAV, with a nearly 1-fold increase in risk (adjusted HR, 1.91; 95% CI, 1.01–3.60). Female AAV patients were 3.24× more likely than females without AAV to develop TB (adjusted HR 3.24; 95% CI, 1.85–5.67). Conclusions: Patients with AAV exhibit a 48% elevated TB risk, notably, a 91% increase within the first two years postdiagnosis. Female AAV patients face a 3.24 times higher TB risk compared to females without AAV. This study is limited by potential misclassification and overestimation of AAV cases. Clinicians should closely monitor TB risk in AAV patients, especially in females and the initial two years following diagnosis.
Primary Melanoma Histopathologic Predictors of Sentinel Lymph Node Positivity: A Proposed Scoring System for Risk Assessment and Patient Selection in a Clinical Setting
Authors: Jeremić Jelena , Radenović Kristina , Jurišić Milana , Suđecki Branko , Marinković Milana , Mihaljević Jovan , Radosavljević Ivan , Jovanović Milan , Stojanović Marina , Milić Nataša , Pavlović Vedrana , Stojičić Milan , Inić Zorka , Jović Marko
Published: October 2023

Background and Objectives: The careful selection of adequate SLNB candidates not only aims at reducing the surgical risk while identifying SLN metastasis, but also plays a crucial role in identifying the patients eligible for adjuvant therapy. Objectives: The purpose of our study was to investigate the clinical and histologic aspects of primary melanomas that correlate with the likelihood of a positive SLNB result. Materials and Methods: A total of 101 primary melanoma patients who underwent sentinel lymph node biopsies were included in the study. General patient demographics were obtained as well as localization and melanoma-specific characteristics of primary melanoma from histologic reports in addition to data derived from SLNB melanoma histopathology reports. Results: The patients with positive SLN results had a statistically significant increased Breslow thickness (3.8 mm vs. 1.97 mm, p = 0.002), higher mitotic index rate (5/mm2 vs. 2/mm2, p = 0.009), as well as the presence of ulceration (68.4% vs. 31.6%, p = 0.007). Univariate regression analysis showed the Breslow thickness (p = 0.008), the mitotic index rate (p = 0.054), the presence of ulceration (p = 0.009), as well as the pT3-4 stage (p = 0.009) to be significant predictors of SLN positivity. The optimal cut-off values for Breslow thickness and the number of mitoses scores were determined based on ROC curve analysis. Using the Breslow thickness, mitotic index rate, presence of ulceration, and pT3-4 stage significant coefficients from the univariate regression model, a chance prediction score was developed. Conclusions: The newly developed and proposed scoring system can aid in patient selection for SLN biopsy by facilitating a more efficient risk assessment in the detection of lymph node metastases in melanoma patients.
In Silico and In Vitro Exploration of Poziotinib and Olmutinib Synergy in Lung Cancer: Role of hsa-miR-7-5p in Regulating Apoptotic Pathway Marker Genes
Authors: Alamery Salman , AlAjmi Anfal , Wani Tanveer A. , Zargar Seema
Published: October 2023

Background and objectives: Non-small cell lung cancer (NSCLC) is often caused by EGFR mutations, leading to overactive cell growth pathways. Drug resistance is a significant challenge in lung cancer treatment, affecting therapy effectiveness and patient survival. However, combining drugs in research shows promise in addressing or delaying resistance, offering a more effective approach to cancer treatment. In this study, we investigated the potential alterations in the apoptotic pathway in A549 cells induced by a combined targeted therapy using tyrosine kinase inhibitors (TKIs) olmutinib and poziotinib, focusing on cell proliferation, differential gene expression, and in silico analysis of apoptotic markers. Methods: A combined targeted therapy involving olmutinib and poziotinib was investigated for its impact on the apoptotic pathway in A549 cells. Cell proliferation, quantitative differential gene expression, and in silico analysis of apoptotic markers were examined. A549 cells were treated with varying concentrations (1, 2.5, and 5 μM) of poziotinib, olmutinib, and their combination. Results: Treatment with poziotinib, olmutinib, and their combination significantly reduced cell proliferation, with the most pronounced effect at 2.5 μM (p < 0.005). A synergistic antiproliferative effect was observed with the combination of poziotinib and olmutinib (p < 0.0005). Quantitative differential gene expression showed synergistic action of the drug combination, impacting key apoptotic genes including STK-11, Bcl-2, Bax, and the Bax/Bcl-2 ratio. In silico analysis revealed direct interactions between EGFR and ERBB2 genes, accounting for 77.64% of their interactions, and 8% co-expression with downstream apoptotic genes. Molecular docking indicated strong binding of poziotinib and olmutinib to extrinsic and intrinsic apoptotic pathway markers, with binding energies of −9.4 kcal/mol and −8.5 kcal/mol, respectively, on interacting with STK-11. Conclusions: Combining poziotinib and olmutinib therapies may significantly improve drug tolerance and conquer drug resistance more effectively than using them individually in lung cancer patients, as suggested by this study’s mechanisms.
Cardiac Magnetic Resonance Left Ventricular Filling Pressure Is Associated with NT-proBNP in Patients with New Onset Heart Failure
Authors: Assadi Hosamadin , Matthews Gareth , Chambers Bradley , Grafton-Clarke Ciaran , Shabi Mubien , Plein Sven , Swoboda Peter P , Garg Pankaj
Published: October 2023

Background and Objectives: Cardiovascular magnetic resonance (CMR) is emerging as an important imaging tool for sub-phenotyping and estimating left ventricular (LV) filling pressure (LVFP). The N-terminal prohormone of B-type natriuretic peptide (NT-proBNP) is released from cardiac myocytes in response to mechanical load and wall stress. This study sought to investigate if CMR-derived LVFP is associated with the serum levels of NT-proBNP and, in addition, if it provides any incremental prognostic value in heart failure (HF). Materials and Methods: This study recruited 380 patients diagnosed with HF who underwent same-day CMR and clinical assessment between February 2018 and January 2020. CMR-derived LVFP was calculated, as previously, from long- and short-axis cines. During CMR assessment, serum NT-proBNP was measured. The pathological cut-offs were defined as follows: NT-proBNP ≥ 125 pg/mL and CMR LVFP > 15 mmHg. The incidence of HF hospitalisation was treated as a clinical outcome. Results: In total, 305 patients had NT-proBNP ≥ 125 pg/mL. Patients with raised NT-proBNP were older (54 ± 14 vs. 64 ± 11 years, p < 0.0001). Patients with raised NT-proBNP had higher LV volumes and mass. In addition, CMR LVFP was higher in patients with raised NT-proBNP (13.2 ± 2.6 vs. 15.4 ± 3.2 mmHg, p < 0.0001). The serum levels of NT-proBNP were associated with CMR-derived LVFP (R = 0.42, p < 0.0001). In logistic regression analysis, this association between NT-proBNP and CMR LVFP was independent of all other CMR variables, including LV ejection fraction, LV mass, and left atrial volume (coefficient = 2.02, p = 0.002). CMR LVFP demonstrated an independent association with the incidence of HF hospitalisation above NT-proBNP (hazard ratio 2.7, 95% confidence interval 1.2 to 6, p = 0.01). Conclusions: A CMR-modelled LVFP is independently associated with serum NT-proBNP levels. Importantly, it provides an incremental prognostic value over and above serum NT-proBNP levels.
Paragangliomas and Anemia: Literature Review and Case Report
Authors: Tănăsescu Maria-Daniela , Popescu Ștefan , Mincă Alexandru , Isac Teodora , Suliman Emel , Grigorie Maria Mihaela , Suliman Emine , Stăniloaie Daniel , Timofte Delia , Ionescu Dorin
Published: October 2023

Paragangliomas are rare neuroendocrine tumors that arise from the extra-adrenal autonomic paraganglia, i.e., small organs consisting mainly of neuroendocrine cells that are derived from the embryonic neural crest and have the ability to secrete catecholamines. Paragangliomas can derive from either parasympathetic or sympathetic paraganglia. Most of the parasympathetic ganglia-derived paragangliomas are nonfunctional, and symptoms result from mass effect. Conversely, the sympathetic paragangliomas are functional and produce catecholamine. Although such patients could have symptoms similar to pheochromocytoma, mass effect symptoms, or non-specific symptoms, being benign tumors, they can also present with anemia, specifically iron-deficiency anemia. Considering that neoplastic pathology is chronically accompanied by moderate, normochromic, normocytic anemia, association between paragangliomas that are mostly benign but with a potential degree of malignancy and anemia is not as frequent as expected, with only 12 cases reported in the literature. We report a case of a 54-year-old female patient diagnosed with a paraganglioma of the carotid glomus accompanied by severe normochromic, normocytic anemia, which reached normal limits after excision of the paraganglioma.
Predictive Modeling of Photovoltaic Panel Power Production through On-Site Environmental and Electrical Measurements Using Artificial Neural Networks
Authors: Lobato-Nostroza Oscar , Chávez-Campos Gerardo Marx , Morales-Cervantes Antony , Chiaradia-Masselli Yvo Marcelo , Lara-Hernández Rafael , Téllez-Anguiano Adriana del Carmen , Fraga-Aguilar Miguelangel
Published: October 2023

Weather disturbances pose a significant challenge when estimating the energy production of photovoltaic panel systems. Energy production and forecasting models have recently been used to improve energy estimations and maintenance tasks. However, these models often rely on environmental measurements from meteorological units far from the photovoltaic systems. To enhance the accuracy of the developed model, a measurement Internet of Things (IoT) prototype was developed in this study, which collects on-site voltage and current measurements from the panel, as well as the environmental factors of lighting, temperature, and humidity in the system’s proximity. The measurements were then subjected to correlation analysis, and various artificial neural networks (ANNs) were implemented to develop energy estimations and forecasting models. The most effective model utilizes lighting, temperature, and humidity. The model achieves a root mean squared error (RMSE) of 0.255326464. The ANN models are compared to an MLR model using the same data. Using previous power measurements and actual weather data, a non-autoregressive neural network (Non-AR-NN) model forecasts future output power values. The best Non-AR-NN model produces an RMSE of 0.1160, resulting in accurate predictions based on the IoT device.
Evaluation of GABA Production by Alginate-Microencapsulated Fresh and Freeze-Dried Bacteria Enriched with Monosodium Glutamate during Storage in Chocolate Milk
Authors: Youssef Hebat Allah Ibrahim , Vitaglione Paola , Ferracane Rosalia , Abuqwider Jumana , Mauriello Gianluigi
Published: October 2023

Two strains of γ-aminobutyric acid (GABA) producing bacteria, L. brevis Y1 and L. plantarum LM2, were microencapsulated in sodium alginate with two concentrations (1% and 2%) of monosodium glutamate (MSG) by using vibrating technology. The mix of both species was microencapsulated both in fresh and freeze-dried form. After 0, 1, 2, and 4 weeks of storage at 4 °C in quarter strength Ringer’s solution, the microcapsules were subjected to cell viable counting and sub-cultured in MRS at 37° for 24 h. The MRS cultures were analyzed for the GABA content. The amount of GABA produced per CFU of MRS inoculum was then calculated. Only the 4-week-old microcapsules were used to inoculate a chocolate milk drink with the aim of obtaining a functionalized drink containing viable probiotic cells and GABA after a 1-week incubation at 4 °C. Therefore, the GABA production in chocolate milk per CFU of the probiotic culture after the incubation time was calculated. Results of the GABA analysis by liquid chromatography mass spectrometry of the MRS sub-cultures showed no significant difference (p > 0.05) in GABA yield between 1% and 2% MSG for the microcapsules containing fresh cells. On the contrary, a significant difference (p < 0.05) in productivity along the storage was registered. Microcapsules containing freeze-dried cells showed significant differences (p < 0.05) in GABA yield between 1% and 2% MSG only after 2 and 4 weeks of storage. A significant difference (p < 0.05) in GABA yield between the storage time was found only for the trials with 2% MSG for freeze-dried cells. The synthesis of GABA in chocolate milk significantly decreased (p < 0.05) only for fresh cells when comparing 2% with 1% MSG. In conclusion, a 1-month storage of microcapsules containing both culture forms, fresh and freeze-dried, did not affect GABA production.
Prediction, Synthesis and Evaluation of a Synthetic Peptide as an Enzyme-Linked Immunosorbent Assay (ELISA) Candidate for Screening of Bovine Antibodies against Theileria annulata
Authors: Mishra Prasanta Kumar Koustasa , Jena Anupama , Sarkhel Souti Prasad , Behera Sujit Kumar , Das Annada , Sabarinath Thankappan , Jena Dayanidhi , Mandal Kruti Debnath , Sahu Adhikari , Kumar Anshuman , Kumar Vinod , Kadam Rahul Ganpatrao , Sathapathy Srinivas , Rao Thavitiki Prasada
Published: October 2023

Tick-borne diseases (TBDs) of livestock are endemic across various parts of tropical countries. Theileriosis is one such economically important TBD, caused by the Theileriidae family of organisms, which is transmitted by ticks. Theileria annulata, the causative agent of tropical theileriosis, contributes a significant loss to the dairy sector by causing anorexia, high fever, anemia, inflammatory changes in vital organs and icterus, thus, a loss in milk yield. Though vaccines are available, their protective efficacy is not absolute, and treatment is limited to early diagnosis of the causative agent. Routinely, microscopic identification of piroplasms in the erythrocytes (Giemsa-stained) of infected animals or schizonts in lymph node biopsies are practiced for diagnosis. PCR-based techniques (multiplex, uniplex, nested and real-time) have been reported to perform well in diagnosing active infection. Several attempts have been made using serological assays like Dot blot, ELISA and ICT, but the results were of variable sensitivity and specificity. Recombinant proteins like the Theileria annulata merozoite surface antigen (Tams1) and Theileria annulata surface protein (TaSP) have been explored as antigenic candidates for these assays. In the present study, we predicted an immunogenic peptide, i.e., TaSP-34, from the TaSP using various computational tools. The predicted peptide was custom synthesized. The diagnostic potential of the peptide was assessed by indirect plate ELISA to detect the bovine-IgM against Theileria annulata. Alongside, a recombinant truncated TaSP (rTaSP(tr)) was expressed and purified, which was used to compare the performance of the peptide as a diagnostic candidate. The IgM-based peptide ELISA was 100% sensitive and 92.77% specific as compared to PCR (Tams1 targeting), while 98.04% sensitivity and 97.44% specificity were observed in comparison with rTaSP(tr) ELISA. Almost perfect agreement between peptide ELISA and Tams1 PCR was observed with a Cohen’s kappa coefficient (κ-value) of 0.901 and agreement of 95.31%. Further, the κ-value between the peptide ELISA and rTaSP(tr) ELISA was found to be 0.95, and the agreement was 97.65%, which shows a good correlation between the two tests. The findings suggest that the TaSP-34 peptide can be an efficient and new-generation diagnostic candidate for the diagnosis of T. annulata. Furthermore, the peptide can be synthesized commercially at a larger scale and can be a cost-effective alternative for the protein-based diagnostic candidates for T. annulata.
Insect Microbial Symbionts: Ecology, Interactions, and Biological Significance
Authors: Mondal Sankhadeep , Somani Jigyasa , Roy Somnath , Babu Azariah , Pandey Abhay K.
Published: October 2023

The guts of insect pests are typical habitats for microbial colonization and the presence of bacterial species inside the gut confers several potential advantages to the insects. These gut bacteria are located symbiotically inside the digestive tracts of insects and help in food digestion, phytotoxin breakdown, and pesticide detoxification. Different shapes and chemical assets of insect gastrointestinal tracts have a significant impact on the structure and makeup of the microbial population. The number of microbial communities inside the gastrointestinal system differs owing to the varying shape and chemical composition of digestive tracts. Due to their short generation times and rapid evolutionary rates, insect gut bacteria can develop numerous metabolic pathways and can adapt to diverse ecological niches. In addition, despite hindering insecticide management programs, they still have several biotechnological uses, including industrial, clinical, and environmental uses. This review discusses the prevalent bacterial species associated with insect guts, their mode of symbiotic interaction, their role in insecticide resistance, and various other biological significance, along with knowledge gaps and future perspectives. The practical consequences of the gut microbiome and its interaction with the insect host may lead to encountering the mechanisms behind the evolution of pesticide resistance in insects.
The Mechanism of Transcription Factor Swi6 in Regulating Growth and Pathogenicity of Ceratocystis fimbriata: Insights from Non-Targeted Metabolomics
Authors: Cong Hao , Li Changgen , Wang Yiming , Zhang Yongjing , Ma Daifu , Li Lianwei , Jiang Jihong
Published: October 2023

Ceratocystis fimbriata (C. fimbriata) is a notorious pathogenic fungus that causes sweet potato black rot disease. The APSES transcription factor Swi6 in fungi is located downstream of the cell wall integrity (CWI)-mitogen-activated protein kinase (MAPK) signaling pathway and has been identified to be involved in cell wall integrity and virulence in several filamentous pathogenic fungi. However, the specific mechanisms by which Swi6 regulates the growth and pathogenicity of plant pathogenic fungi remain elusive. In this study, the SWI6 deletion mutants and complemented strains of C. fimbriata were generated. Deletion of Swi6 in C. fimbriata resulted in aberrant growth patterns. Pathogenicity assays on sweet potato storage roots revealed a significant decrease in virulence in the mutant. Non-targeted metabolomic analysis using LC-MS identified a total of 692 potential differentially accumulated metabolites (PDAMs) in the ∆Cfswi6 mutant compared to the wild type, and the results of KEGG enrichment analysis demonstrated significant enrichment of PDAMs within various metabolic pathways, including amino acid metabolism, lipid metabolism, nucleotide metabolism, GPI-anchored protein synthesis, and ABC transporter metabolism. These metabolic pathways were believed to play a crucial role in mediating the growth and pathogenicity of C. fimbriata through the regulation of CWI. Firstly, the deletion of the SWI6 gene led to abnormal amino acid and lipid metabolism, potentially exacerbating energy storage imbalance. Secondly, significant enrichment of metabolites related to GPI-anchored protein biosynthesis implied compromised cell wall integrity. Lastly, disruption of ABC transport protein metabolism may hinder intracellular transmembrane transport. Importantly, this study represents the first investigation into the potential regulatory mechanisms of SWI6 in plant filamentous pathogenic fungi from a metabolic perspective. The findings provide novel insights into the role of SWI6 in the growth and virulence of C. fimbriata, highlighting its potential as a target for controlling this pathogen.