bluuebunny/crossref_metadata_2025 huggingface dataset:
Catalysts Based on Iron Oxides for Wastewater Purification from Phenolic Compounds: Synthesis, Physicochemical Analysis, Determination of Catalytic Activity
Authors: Dossumova Binara T. , Sassykova Larissa R. , Shakiyeva Tatyana V. , Muktaly Dinara , Batyrbayeva Aigul A. , Kozhaisakova Madina A.
Published: January 2024

In this work, the synthesis of magnetite nanoparticles and catalysts based on it stabilized with silicon and aluminum oxides was carried out. It is revealed that the stabilization of the magnetite surface by using aluminum and silicon oxides leads to a decrease in the size of magnetite nanocrystals in nanocomposites (particle diameter less than ~10 nm). The catalytic activity of the obtained catalysts was evaluated during the oxidation reaction of phenol, pyrocatechin and cresol with oxygen. It is well known that phenolic compounds are among the most dangerous water pollutants. The effect of phenol concentration and the effect of temperature (303–333 K) on the rate of oxidation of phenol to Fe3O4/SiO2 has been studied. It has been determined that the dependence of the oxidation rate of phenol on the initial concentration of phenol in solution is described by a first-order equation. At temperatures of 303–313 K, incomplete absorption of the calculated amount of oxygen is observed, and the analysis data indicate the non-selective oxidation of phenol. Intermediate products, such as catechin, hydroquinone, formic acid, oxidation products, were found. The results of UV and IR spectroscopy showed that catalysts based on magnetite Fe3O4 are effective in the oxidation of phenol with oxygen. In the UV spectrum of the product in the wavelength range 190–1100 nm, there is an absorption band at a wavelength of 240–245 nm and a weak band at 430 nm, which is characteristic of benzoquinone. In the IR spectrum of the product, absorption bands were detected in the region of 1644 cm−1, which is characteristic of the oscillations of the C=O bonds of the carbonyl group of benzoquinone. The peaks also found at 1353 cm−1 and 1229 cm−1 may be due to vibrations of the C-H and C-C bonds of the quinone ring. It was found that among the synthesized catalysts, the Fe3O4/SiO2 catalyst demonstrated the greatest activity in the reaction of liquid-phase oxidation of phenol.
What Predicts Long-Term Absenteeism, and Who Disappears from the Workforce When Enterprises Downsize?
Authors: Aarstad Jarle , Kvitastein Olav Andreas
Published: January 2024

This paper primarily studies how wages predict long-term absenteeism in enterprises. In addition, it studies who disappears from the workforce when downsizing. Analyzing Norwegian enterprise data using dynamic unconditional quasi-maximum likelihood fixed-effects panel regression and general methods of moments panel regression with instrumental variables, we find that increasing average wages decreases average long-term absenteeism. As the effect barely abates the following year, it likely reflects highly skilled and motivated employees in good health receiving a wage premium and not a stimulus boosting overall work attitudes, which is likely short-lived. Wage inequality increases absenteeism, indicating that increasing low earners’ wages relative to those earning high ones decreases absenteeism, but the effect is short-lived and disappears the following year. In addition, average age and education tend to decrease absenteeism, but female labor participation increases it, likely due to maternity leave. Also, increasing the workforce increases absenteeism, indicating that handling many new employees is challenging. When enterprises downsize, young and low earners initially disappear from the workforce, but the following year, older and high earners share the same fate.
Disaggregating Longer-Term Trends from Seasonal Variations in Measured PV System Performance
Authors: Okorieimoh Chibuisi Chinasaokwu , Norton Brian , Conlon Michael
Published: January 2024

Photovoltaic (PV) systems are widely adopted for renewable energy generation, but their performance is influenced by complex interactions between longer-term trends and seasonal variations. This study aims to remove these factors and provide valuable insights for optimising PV system operation. We employ comprehensive datasets of measured PV system performance over five years, focusing on identifying the distinct contributions of longer-term trends and seasonal effects. To achieve this, we develop a novel analytical framework that combines time series and statistical analytical techniques. By applying this framework to the extensive performance data, we successfully break down the overall PV system output into its constituent components, allowing us to find out the impact of the system degradation, maintenance, and weather variations from the inherent seasonal patterns. Our results reveal significant trends in PV system performance, indicating the need for proactive maintenance strategies to mitigate degradation effects. Moreover, we quantify the impact of changing weather patterns and provide recommendations for optimising the system’s efficiency based on seasonally varying conditions. Hence, this study not only advances our understanding of the intricate variations within PV system performance but also provides practical guidance for enhancing the sustainability and effectiveness of solar energy utilisation in both residential and commercial settings.
Novel Hybrid SOR- and AOR-Based Multi-User Detection for Uplink M-MIMO B5G Systems
Authors: Tu Yung-Ping , Jian Pei-Shen , Huang Yung-Fa
Published: January 2024

The Internet of Things (IoT) is one of the most important wireless sensor network (WSN) applications in 5G systems and requires a large amount of wireless data transmission. Therefore, massive multiple-input multiple-output (M-MIMO) has become a crucial type of technology and trend in the future of beyond fifth-generation (B5G) wireless network communication systems. However, as the number of antennas increases, this also causes a significant increase in complexity at the receiving end. This is a challenge that must be overcome. To reduce the BER, confine the computational complexity, and produce a form of detection suitable for 4G and B5G environments simultaneously, we propose a novel multi-user detection (MUD) scheme for the uplink of M-MIMO orthogonal frequency division multiplexing (OFDM) and universal filtered multi-carrier (UFMC) systems that combines the merits of successive over-relaxation (SOR) and accelerated over-relaxation (AOR) named mixed over-relaxation (MOR). Herein, we divide MOR into the initial and collaboration stages. The former will produce the appropriate initial parameters to improve feasibility and divergence risk. Then, the latter achieves rapid convergence and refinement performance through alternating iterations. The conducted simulations show that our proposed form of detection, compared with the BER performance of traditional SOR and AOR, can achieve 99.999% and 99.998% improvement, respectively, and keep the complexity at O(N2). It balances BER performance and complexity with fewer iterations.
Demagnetization Fault Diagnosis of a PMSM for Electric Drilling Tools Using GAF and CNN
Authors: Zhang Qingxue , Cui Junguo , Xiao Wensheng , Mei Lianpeng , Yu Xiaolong
Published: January 2024

Permanent magnets (PMs) provide high efficiency for synchronous motors used for driving drilling tools. Demagnetization is a special fault that reduces the efficiency of the permanent magnet synchronous motor (PMSM) and thus affects the performance of the drilling tools. Therefore, early detection of demagnetization is important for safe and efficient operation. However, it is difficult to detect multiple demagnetization types at the same time using traditional fault diagnosis methods, and the recognition accuracy cannot be guaranteed. To solve the above problem, this article proposes a method combining Gramian angular field (GAF) transform and convolutional neural network (CNN) to recognize and classify different types of demagnetization faults based on output torque signal. Firstly, the thermal demagnetization model of PM was obtained by experiments, and the finite element model (FEM) of PMSM for electric drilling tools was established to analyze the torque, back electromotive force (BEMF), and air gap flux density under different demagnetization faults. Then, the acquired one-dimensional torque signals were transformed into two-dimensional gray images based on the GAF method to enhance the fault features. To improve the generalization ability of the CNN, these gray images were augmented through increasing noise. Finally, the CNN structure was designed and trained with a training accuracy of 98.33%, and the effectiveness of the method was verified by the demagnetization fault experiment. The results show that the testing accuracy of the CNN model was 97.41%, indicating the proposed method can diagnose various demagnetization faults effectively, and that it is immune to loads.
Top-k Graph Similarity Search Algorithm Based on Chi-Square Statistics in Probabilistic Graphs
Authors: Chen Ziyang , Zhuang Junhao , Wang Xuan , Tang Xian , Yang Kun , Du Ming , Zhou Junfeng
Published: January 2024

Top-k graph similarity search on probabilistic graphs is widely used in various scenarios, such as symptom–disease diagnostics, community discovery, visual pattern recognition, and communication networks. The state-of-the-art method uses the chi-square statistics to speed up the process. The effectiveness of the chi-square statistics solution depends on the effectiveness of the sample observation and expectation. The existing method assumes that the labels in the data graphs are subject to uniform distribution and calculate the chi-square value based on this. In fact, however, the actual distribution of the labels does not meet the requirement of uniform distribution, resulting in a low quality of the returned results. To solve this problem, we propose a top-k similar subgraph search algorithm ChiSSA based on chi-square statistics. We propose two ways to calculate the expectation vector according to the actual distribution of labels in the graph, including the local expectation calculation method based on the vertex neighbors and the global expectation calculation method based on the label distribution of the whole graph. Furthermore, we propose two optimization strategies to improve the accuracy of query results and the efficiency of our algorithm. We conduct rich experiments on real datasets. The experimental results on real datasets show that our algorithm improves the quality and accuracy by an average of 1.66× and 1.68× in terms of time overhead, it improves by an average of 3.41×.
Proposed Extension of the U.S.–Caribbean Super Grid to South America for Resilience during Hurricanes
Authors: Itiki Rodney , Manjrekar Madhav , Di Santo Silvio Giuseppe
Published: January 2024

Climate change mitigation, adaptation to intensifying hurricanes, and decarbonization challenges in developing countries emphasize the urgent need for resilient high-voltage grids to facilitate the expansion of renewables. This research explores the technical feasibility of extending the U.S.–Caribbean Super Grid to include the Virgin Islands, Guadeloupe, Martinique, Trinidad and Tobago, Guyana, Suriname, French Guyana, and the northeastern part of Brazil in South America. This proposed extension aims to capitalize on the recent introduction of a new generation of wind turbines certified for operation under strong hurricane forces. The research utilizes modeling and simulation techniques to evaluate the performance of the proposed extension. A method for modeling and estimating spatiotemporal wind power profiles is applied, and the results demonstrate a reduction in maximum wind power variability within the U.S.–Caribbean Super Grid. Depending on the hurricane trajectory, the variability is reduced from 56.6% to less than 43.2%. This reduction takes effect by distributing peak surplus wind power alongside the proposed U.S.–Caribbean–South America Super Grid (UCASG). The research concludes by acknowledging the merits and limitations of the study and discussing potential directions for future research in this field.
Sustainability Assessment of Highly Fluorescent Carbon Dots Derived from Eucalyptus Leaves
Authors: Johny Archana , Pinto da Silva Luís , Pereira Carlos M. , Esteves da Silva Joaquim C. G.
Published: January 2024

Biomass-derived carbon dots (CDs) are gaining much interest in recent times, as they provide a sustainable option with abundant availability, a low cost and tunable luminescence. Herein, we report a simple green synthesis method to produce highly fluorescent CDs from Eucalyptus globulus leaves using the one-pot hydrothermal approach. The fabricated CDs exhibit strong blue fluorescence with an excitation and emission maxima of 320 nm and 445 nm, respectively. The highest quantum yield (QY) obtained was 60.7%. With the reported optical properties and biocompatibility, CDs can be looked at as a promising candidate for potential biosensing applications. Moreover, we employed a life cycle assessment (LCA) cradle-to-gate approach to study the environmental impacts of the synthesis strategy used for the fabrication of CDs. The results point out that citric acid is the main hotspot in CD synthesis, regarding environmental impacts in most categories. This justifies the introduction of biomass, which reduces the amount of citric acid, thus leading to a more sustainable synthesis strategy for fabricating CDs.
How Much Visual Greenery Can Street Trees Generate from a Humanistic Perspective? An Attempt to Quantify the Canopy Green View Index Based on Tree Morphology
Authors: Zhu Huaizhen , Nan Xinge , Kang Ning , Li Shuhua
Published: January 2024

Street trees are essential to urban ecological benefits and human well-being. The canopy morphology relates to the green view index (GVI), which needs to be calculated based on specific tree species. This study conducts a field study on 760 street trees of 3 species from the pedestrian perspective, and explores the differences in canopy GVI and its factors of different specifications of street trees using DBH grading. The results indicate that (1) street trees can provide 20% of the GVI in unilateral streets, with 13% of the GVI in a single canopy. (2) A flat oval crown with a wider canopy width is more effective in providing GVI than a long oval crown, and a CW with a higher canopy GVI highlights this advantage. (3) DBHs of 30 and 40 can be used as grading indicators for Cinnamomum camphora, and the specification requirements can be reduced for Southern magnolia and Chinese privet. (4) The concept of DE is introduced, and new parameters related to the central crown have significant impacts on GVI. The conclusions can improve the GVI application in urban greenery planning as well as have certain implications for the cost management of future seedling cultivation.
Controllable Queuing System with Elastic Traffic and Signals for Resource Capacity Planning in 5G Network Slicing
Authors: Kochetkova Irina , Leonteva Kseniia , Ghebrial Ibram , Vlaskina Anastasiya , Burtseva Sofia , Kushchazli Anna , Samouylov Konstantin
Published: January 2024

Fifth-generation (5G) networks provide network slicing capabilities, enabling the deployment of multiple logically isolated network slices on a single infrastructure platform to meet specific requirements of users. This paper focuses on modeling and analyzing resource capacity planning and reallocation for network slicing, specifically between two providers transmitting elastic traffic, such during as web browsing. A controller determines the need for resource reallocation and plans new resource capacity accordingly. A Markov decision process is employed in a controllable queuing system to find the optimal resource capacity for each provider. The reward function incorporates three network slicing principles: maximum matching for equal resource partitioning, maximum share of signals resulting in resource reallocation, and maximum resource utilization. To efficiently compute the optimal resource capacity planning policy, we developed an iterative algorithm that begins with maximum resource utilization as the starting point. Through numerical demonstrations, we show the optimal policy and metrics of resource reallocation for two services: web browsing and bulk data transfer. The results highlight fast convergence within three iterations and the effectiveness of the balanced three-principle approach in resource capacity planning for 5G network slicing.
Characteristics of Quinoa Protein Isolate Treated by Pulsed Electric Field
Authors: Zhang Xinyue , Li Zhanrong , Zheng Xiaojiao , Wen Wenjun , Wang Xiaowen
Published: January 2024

The aim of this study was to investigate the impact of a pulsed electric field (PEF) on the structural and functional properties of quinoa protein isolate (QPI). The findings revealed a significant alteration in the secondary structure of QPI following PEF treatment, converting the random coil into the β-sheet, resulting in an improvement in structure orderliness and an enhancement of thermal stability. The PEF treatment led to a reduction in particle size, induced structural unfolding, and increased the surface hydrophobicity, resulting in a statistically significant enhancement in the solubility, foaming, and emulsifying properties of QPI (p < 0.05). Specifically, PEF treatment at 7.5 kV/cm for 30 pulses was identified as the optimal condition for modifying QPI. This study provides a basis for the precision and range of application of pulsed electric field treatment and offers the possibility of improving the physical and chemical properties of quinoa protein.
Integral Use of Red Wine Pomace after Hydrostatic High Pressure: Application of Two Consecutive Cycles of Treatment
Authors: D’Arrigo Matilde , Delgado-Adámez Jonathan , Rocha-Pimienta Javier , Valdés-Sánchez M. Esperanza , Ramírez-Bernabé M. Rosario
Published: January 2024

The influence of applying hydrostatic high pressure (HHP) to red grape pomace cv. Tempranillo was studied to obtain an ingredient rich in bioactive compounds for the manufacture of food products. Four treatments were investigated: (i) 600 MPa/1 s; (ii) 600 MPa/300 s, and other two treatments with 2 cycles of HHP: (iii) 2 cycles of 600 MPa/1 s; and (iv) 1 first cycle of 400 MPa/1 s and a second cycle 600 MPa/1 s. Treated pomace was stored at different temperatures (4 and 20 °C). The application of two consecutive cycles had no effect on the microorganisms’ inactivation compared to only one cycle. Immediately after HHP, the phenolic compounds content was maintained. However, HHP had no influence on the polyphenol oxidase enzyme (PPO), and so the phenolic compounds were significantly reduced during storage. Hence, the shelf-life of red grape pomace was significantly reduced at both temperatures, although phenolic compounds were better preserved under refrigeration than at room temperature.
From Water for Water: PEDOT:PSS-Chitosan Beads for Sustainable Dyes Adsorption
Authors: Vassalini Irene , Maddaloni Marina , Depedro Mattia , De Villi Alice , Ferroni Matteo , Alessandri Ivano
Published: January 2024

This study investigates the viability of developing chitosan-based hydrogels derived from waste shrimp shells for the removal of methylene blue and methyl orange, thereby transforming food waste into advanced materials for environmental remediation. Despite chitosan-based adsorbents being conventionally considered ideal for the removal of negative pollutants, through targeted functionalization with poly(3,4-ethylenedioxythiophene): polystyrene sulfonate (PEDOT:PSS) at varying concentrations, we successfully enhance the hydrogels’ efficacy in also adsorbing positively charged adsorbates. Specifically, the incorporation of PEDOT:PSS at a concentration of 10% v/v emerges as a critical factor in facilitating the robust adsorption of dyes. In the case of the anionic dye methyl orange (MO, 10−5 M), the percentage of removed dye passed from 47% (for beads made of only chitosan) to 66% (for beads made of chitosan-PEDOT:PSS 10%), while, in the case of the cationic dye methylene blue (MB, 10−5 M), the percentage of removed dye passed from 52 to 100%. At the basis of this enhancement, there is an adsorption mechanism resulting from the interplay between electrostatic forces and π–π interactions. Furthermore, the synthesized functionalized hydrogels exhibit remarkable stability and reusability (at least five consecutive cycles) in the case of MB, paving the way for the development of cost-effective and sustainable adsorbents. This study highlights the potential of repurposing waste materials for environmental benefits, introducing an innovative approach to address the challenges regarding water pollution.
Genomic and Functional Evaluation of Two Lacticaseibacillus paracasei and Two Lactiplantibacillus plantarum Strains, Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis), as Probiotics for Aquaculture
Authors: Contente Diogo , Díaz-Formoso Lara , Feito Javier , Hernández Pablo E. , Muñoz-Atienza Estefanía , Borrero Juan , Poeta Patrícia , Cintas Luis M.
Published: January 2024

Aquaculture plays a crucial role in meeting the increasing global demand for food and protein sources. However, its expansion is followed by increasing challenges, such as infectious disease outbreaks and antibiotic misuse. The present study focuses on the genetic and functional analyses of two Lacticaseibacillus paracasei (BF3 and RT4) and two Lactiplantibacillus plantarum (BF12 and WT12) strains isolated from a rotifer cultivation tank used for turbot larviculture. Whole-genome sequencing (WGS) and bioinformatics analyses confirmed their probiotic potential, the absence of transferable antibiotic resistance genes, and the absence of virulence and pathogenicity factors. Bacteriocin mining identified a gene cluster encoding six plantaricins, suggesting their role in the antimicrobial activity exerted by these strains. In vitro cell-free protein synthesis (IV-CFPS) analyses was used to evaluate the expression of the plantaricin genes. The in vitro-synthesized class IIb (two-peptide bacteriocins) plantaricin E/F (PlnE/F) exerted antimicrobial activity against three indicator microorganisms, including the well-known ichthyopathogen Lactococcus garvieae. Furthermore, MALDI-TOF MS on colonies detected the presence of a major peptide that matches the dimeric form of plantaricins E (PlnE) and F (PlnF). This study emphasizes the importance of genome sequencing and bioinformatic analysis for evaluating aquaculture probiotic candidates. Moreover, it provides valuable insights into their genetic features and antimicrobial mechanisms, paving the way for their application as probiotics in larviculture, which is a major bottleneck in aquaculture.
Can Clinical Assessment of Postural Control Explain Locomotive Body Function, Mobility, Self-Care and Participation in Children with Cerebral Palsy?
Authors: Vlčkova Blanka , Halámka Jiří , Müller Markus , Sanz-Mengibar Jose Manuel , Šafářová Marcela
Published: January 2024

Trunk control may influence self-care, mobility, and participation, as well as how children living with cerebral palsy (CP) move around. Mobility and Gross Motor performance are described over environmental factors, while locomotion can be understood as the intrinsic ontogenetic automatic postural function of the central nervous system, and could be the underlying element explaining the relationship between these factors. Our goal is to study the correlation among Trunk Control Measurement Scale (TCMS) and Pediatric Evaluation of Disability Inventory (PEDI) domains, as well as Locomotor Stages (LS). Methods: A feasibility observational analysis was designed including 25 children with CP who were assessed with these scales. Results: The strong correlation confirms higher levels of trunk control in children with better self-care, mobility and participation capacities. Strong correlations indicate also that higher LS show better levels of PEDI and TCMS domains. Conclusions: Our results suggest that more mature LS require higher levels of trunk control, benefitting self-care, mobility and social functions.
Non-Coding RNAs as Key Regulators in Lung Cancer
Authors: Gilyazova Irina , Gimalova Galiya , Nizamova Aigul , Galimova Elmira , Ishbulatova Ekaterina , Pavlov Valentin , Khusnutdinova Elza
Published: January 2024

For several decades, most lung cancer investigations have focused on the search for mutations in candidate genes; however, in the last decade, due to the fact that most of the human genome is occupied by sequences that do not code for proteins, much attention has been paid to non-coding RNAs (ncRNAs) that perform regulatory functions. In this review, we principally focused on recent studies of the function, regulatory mechanisms, and therapeutic potential of ncRNAs including microRNA (miRNA), long ncRNA (lncRNA), and circular RNA (circRNA) in different types of lung cancer.
Probiotic Bacillus licheniformis ZW3 Alleviates DSS-Induced Colitis and Enhances Gut Homeostasis
Authors: Jia Dan , Li Yingying , Wang Yingjie , Guo Yanan , Liu Junlong , Zhao Shuaiyang , Wang Jinming , Guan Guiquan , Luo Jianxun , Yin Hong , Tang Lijie , Li Youquan
Published: January 2024

Despite Bacillus species having been extensively utilized in the food industry and biocontrol as part of probiotic preparations, limited knowledge exists regarding their impact on intestinal disorders. In this study, we investigated the effect of Bacillus licheniformis ZW3 (ZW3), a potential probiotic isolated from camel feces, on dextran sulfate sodium (DSS)-induced colitis. The results showed ZW3 partially mitigated body weight loss, disease activity index (DAI), colon shortening, and suppressed immune response in colitis mice, as evidenced by the reduction in the levels of the inflammatory markers IL-1β, TNF-α, and IL-6 (p < 0.05). ZW3 was found to ameliorate DSS-induced dysfunction of the colonic barrier by enhancing mucin 2 (MUC2), zonula occluden-1 (ZO-1), and occludin. Furthermore, enriched beneficial bacteria Lachnospiraceae_NK4A136_group and decreased harmful bacteria Escherichia-Shigella revealed that ZW3 improved the imbalanced gut microbiota. Abnormally elevated uric acid levels in colitis were further normalized upon ZW3 supplementation. Overall, this study emphasized the protective effects of ZW3 in colitis mice as well as some potential applications in the management of inflammation-related diseases.
Cell-Surface GRP78-Targeted Chimeric Antigen Receptor T Cells Eliminate Lung Cancer Tumor Xenografts
Authors: Wang Shijie , Wei Wenwen , Yuan Yuncang , Guo Jing , Liang Dandan , Zhao Xudong
Published: January 2024

Lung cancer is one of the most common and intractable malignancies. It is associated with low survival rates despite existing treatments, indicating that new and more effective therapies are urgently needed such as the chimeric antigen receptor-T (CAR-T) cell immunotherapy. The cell-surface glucose-regulated protein 78 (csGRP78) is expressed in various hematological malignancies and solid tumor cells including lung cancer in response to cancer-related endoplasmic reticulum stress, while GRP78 is restricted to inside the normal cells. Here, we detected the prominent expression of csGRP78 in both lung cancer cell lines, A549 and H1299, as well as cancer stemlike cells derived from A549 by immunofluorescence. Next, a csGRP78-targeted CAR was constructed, and the transduced CAR-T cells were tested for their potency to kill the two lung cancer cell lines and derived stemlike cells, which was correlated with specific interferon γ release in vitro. Finally, we found that csGRP78 CAR-T cells also efficiently killed both lung cancer cells and cancer stemlike cells, resulting into the elimination of tumor xenografts in vivo, neither with any evidence of relapse after 63 days of tumor clearance nor any detrimental impact on other body organs we examined. Our study reveals the capacity of csGRP78 as a therapeutic target and offers valuable insight into the development of csGRP78 CAR-T cells as potential therapy for lung cancer.
Phytochemistry and Biological Profile of Gaultheria procumbens L. and Wintergreen Essential Oil: From Traditional Application to Molecular Mechanisms and Therapeutic Targets
Authors: Michel Piotr , Olszewska Monika Anna
Published: January 2024

Gaultheria procumbens L. is a medicinal plant whose aerial parts (leaves, stems, and fruits) and methyl salicylate-rich essential oil (wintergreen oil) are used in phytotherapy to treat inflammation, muscular pain, and infection-related disorders. This overview summarises the current knowledge about ethnobotany, phytochemistry, pharmacology, molecular mechanisms, biocompatibility, and traditional use of G. procumbens and the wintergreen oil distilled from different plant organs. Over 70 hydrophilic compounds, including methyl salicylate glycosides, flavonoids, procyanidins, free catechins, caffeoylquinic acids, and simple phenolic acids, have been identified in G. procumbens plant parts. Moreover, aliphatic compounds, triterpene acids, and sterols have been revealed in lipophilic fractions. Furthermore, over 130 volatile compounds have been detected in wintergreen oil with dominating methyl salicylate (96.9–100%). The accumulated research indicates that mainly hydrophilic non-volatiles are responsible for the pharmacological effects of G. procumbens, primarily its potent anti-inflammatory, antioxidant, and photoprotective activity, with mechanisms verified in vitro and ex vivo in cellular and cell-free assays. The biological effectiveness of the dominant methyl salicylate glycoside—gaultherin—has also been confirmed in animals. Wintergreen oil is reported as a potent anti-inflammatory agent exhibiting moderate antioxidant and antimicrobial activity in vitro and significant insecticidal and larvicidal capacity. Together, G. procumbens accumulate a diverse fraction of polyphenols, triterpenes, and volatiles with validated in vitro and ex vivo biological activity but with the absence of in vivo studies, especially clinical trials concerning effective dose determination and toxicological verification and technological research, including drug formulation.
Gut Microbiome Composition and Dynamics in Hospitalized COVID-19 Patients and Patients with Post-Acute COVID-19 Syndrome
Authors: Brīvība Monta , Silamiķele Laila , Birzniece Līga , Ansone Laura , Megnis Kaspars , Silamiķelis Ivars , Pelcmane Līva , Borisova Daniella , Rozenberga Maija , Jagare Lauma , Elbere Ilze , Kloviņš Jānis
Published: January 2024

The gut microbiome plays a pivotal role in the modulation of host responses during viral infections, and recent studies have underscored its significance in the context of coronavirus disease 2019 (COVID-19). We aimed to investigate the dynamics and compositional changes in the gut microbiome of COVID-19 patients, addressing both the acute phase and the recovery process, with a particular focus on the emergence of post-COVID-19 conditions. Involving 146 COVID-19 patients and 110 healthy controls, this study employed a shotgun metagenomics approach for cross-sectional and longitudinal analyses with one- and three-month follow-ups. We observed a decline in taxonomic diversity among hospitalized COVID-19 patients compared to healthy controls, while a subsequent increase in alpha diversity was shown during the recovery process. A notable contribution of Enterococcus faecium was identified in the acute phase of the infection, accompanied by an increasing abundance of butyrate-producing bacteria (e.g., Roseburia, Lachnospiraceae_unclassified) during the recovery period. We highlighted a protective role of the Prevotella genus in the long-term recovery process and suggested a potential significance of population-specificity in the early gut microbiome markers of post-acute COVID-19 syndrome. Our study represents distinctive gut microbiome signatures in COVID-19, with potential diagnostic and prognostic implications, pinpointing potential modulators of the disease progression.
Exploring the Impact of Exosomal Cargos on Osteosarcoma Progression: Insights into Therapeutic Potential
Authors: Chen Claire C. , Benavente Claudia A.
Published: January 2024

Osteosarcoma (OS) is a primary malignant bone tumor with high metastasis. Poor prognosis highlights a clinical need for novel therapeutic strategies. Exosomes, also known as extracellular vesicles, have been identified as essential players in the modulation of cancer. Recent studies have suggested that OS-derived exosomes can drive pro-tumorigenic or anti-tumorigenic phenotypes by transferring specific cargos, including proteins, nucleic acids, and metabolites, to neighboring cells, significantly impacting the regulation of cellular processes. This review discusses the advancement of exosomes and their cargos in OS. We examine how these exosomes contribute to the modulation of cellular phenotypes associated with tumor progression and metastasis. Furthermore, we explore the potential of exosomes as valuable biomarkers for diagnostics and prognostic purposes and their role in shaping innovative therapeutic strategies in OS treatment development.
The Targeted Regulation of BDUbc and BDSKL1 Enhances Resistance to Blight in Bambusa pervariabilis × Dendrocalamopsis grandis
Authors: Yan Peng , Wang Yisi , Yu Cailin , Piao Jingmei , Li Shuying , Liu Yinggao , Li Shujiang
Published: January 2024

Arthrinium phaeospermum is the major pathogen responsible for the significant stem disease “blight” in B. pervariabilis × D. grandis. The interacting proteins of the key pathogenic factor ApCtf1β, BDUbc and BDSKL1, have previously been obtained by two-hybrid, BiFC, GST pull-down yeast assays. However, the functions of these interacting proteins remain unknown. This study successfully obtained transgenic plants overexpressing BDUbc, BDSKL1, and BDUbc + BDSKL1 via Agrobacterium-mediated gene overexpression. qRT-PCR analysis revealed significantly increased expression levels of BDUbc and BDSKL1 in the transgenic plants. After infection with the pathogenic spore suspension, the disease incidence and severity index significantly decreased across all three transgenic plants, accompanied by a marked increase in defense enzyme levels. Notably, the co-transformed plant, OE-BDUbc + BDSKL1, demonstrated the lowest disease incidence and severity index among the transgenic variants. These results not only indicate that BDUbc and BDSKL1 are disease-resistant genes, but also that these two genes may exhibit a synergistic enhancement effect, which further improves the resistance to blight in Bambusa pervariabilis × Dendrocalamopsis grandis.
Investigating the Differential Circulating microRNA Expression in Adolescent Females with Severe Idiopathic Scoliosis: A Proof-of-Concept Observational Clinical Study
Authors: Raimondi Lavinia , De Luca Angela , Gallo Alessia , Perna Fabrizio , Cuscino Nicola , Cordaro Aurora , Costa Viviana , Bellavia Daniele , Faldini Cesare , Scilabra Simone Dario , Giavaresi Gianluca , Toscano Angelo
Published: January 2024

Adolescent Idiopathic Scoliosis (AIS) is the most common form of three-dimensional spinal disorder in adolescents between the ages of 10 and 18 years of age, most commonly diagnosed in young women when severe disease occurs. Patients with AIS are characterized by abnormal skeletal growth and reduced bone mineral density. The etiology of AIS is thought to be multifactorial, involving both environmental and genetic factors, but to date, it is still unknown. Therefore, it is crucial to further investigate the molecular pathogenesis of AIS and to identify biomarkers useful for predicting curve progression. In this perspective, the relative abundance of a panel of microRNAs (miRNAs) was analyzed in the plasma of 20 AIS patients and 10 healthy controls (HC). The data revealed a significant group of circulating miRNAs dysregulated in AIS patients compared to HC. Further bioinformatic analyses evidenced a more restricted expression of some miRNAs exclusively in severe AIS females. These include some members of the miR-30 family, which are considered promising regulators for treating bone diseases. We demonstrated circulating extracellular vesicles (EVs) from severe AIS females contained miR-30 family members and decreased the osteogenic differentiation of mesenchymal stem cells. Proteomic analysis of EVs highlighted the expression of proteins associated with orthopedic disease. This study provides preliminary evidence of a miRNAs signature potentially associated with severe female AIS and suggests the corresponding vesicular component may affect cellular mechanisms crucial in AIS, opening the scenario for in-depth studies on prognostic differences related to gender and grade.
New-Generation Glucokinase Activators: Potential Game-Changers in Type 2 Diabetes Treatment
Authors: Haddad Dania , Dsouza Vanessa Sybil , Al-Mulla Fahd , Al Madhoun Ashraf
Published: January 2024

Achieving glycemic control and sustaining functional pancreatic β-cell activity remains an unmet medical need in the treatment of type 2 diabetes mellitus (T2DM). Glucokinase activators (GKAs) constitute a class of anti-diabetic drugs designed to regulate blood sugar levels and enhance β-cell function in patients with diabetes. A significant progression in GKA development is underway to address the limitations of earlier generations. Dorzagliatin, a dual-acting GKA, targets both the liver and pancreas and has successfully completed two phase III trials, demonstrating favorable results in diabetes treatment. The hepato-selective GKA, TTP399, emerges as a strong contender, displaying clinically noteworthy outcomes with minimal adverse effects. This paper seeks to review the current literature, delve into the mechanisms of action of these new-generation GKAs, and assess their efficacy and safety in treating T2DM based on published preclinical studies and recent clinical trials.
Mitochondrial Dysfunction and Coenzyme Q10 Supplementation in Post-Viral Fatigue Syndrome: An Overview
Authors: Mantle David , Hargreaves Iain Parry , Domingo Joan Carles , Castro-Marrero Jesus
Published: January 2024

Post-viral fatigue syndrome (PVFS) encompasses a wide range of complex neuroimmune disorders of unknown causes characterised by disabling post-exertional fatigue, myalgia and joint pain, cognitive impairments, unrefreshing sleep, autonomic dysfunction, and neuropsychiatric symptoms. It includes myalgic encephalomyelitis, also known as chronic fatigue syndrome (ME/CFS); fibromyalgia (FM); and more recently post-COVID-19 condition (long COVID). To date, there are no definitive clinical case criteria and no FDA-approved pharmacological therapies for PVFS. Given the current lack of effective treatments, there is a need to develop novel therapeutic strategies for these disorders. Mitochondria, the cellular organelles responsible for tissue energy production, have recently garnered attention in research into PVFS due to their crucial role in cellular bioenergetic metabolism in these conditions. The accumulating literature has identified a link between mitochondrial dysfunction and low-grade systemic inflammation in ME/CFS, FM, and long COVID. To address this issue, this article aims to critically review the evidence relating to mitochondrial dysfunction in the pathogenesis of these disorders; in particular, it aims to evaluate the effectiveness of coenzyme Q10 supplementation on chronic fatigue and pain symptoms as a novel therapeutic strategy for the treatment of PVFS.
Lactiplantibacillus plantarum HY7718 Improves Intestinal Integrity in a DSS-Induced Ulcerative Colitis Mouse Model by Suppressing Inflammation through Modulation of the Gut Microbiota
Authors: Kim Hyeon-Ji , Jeon Hye-Jin , Kim Joo-Yun , Shim Jae-Jung , Lee Jae-Hwan
Published: January 2024

Inflammatory bowel disease (IBD), a chronic condition that causes persistent inflammation in the digestive system, is closely associated with the intestinal microbiome. Here, we evaluated the effects of Lactiplantibacillus plantarum HY7718 (HY7718) on IBD symptoms in mice with dextran sulfate sodium (DSS)-induced colitis. Oral administration of HY7718 led to significant improvement in the disease activity index score and the histological index, as well as preventing weight loss, in model mice. HY7718 upregulated the expression of intestinal tight junction (TJ)-related genes and downregulated the expression of genes encoding pro-inflammatory cytokines and genes involved in the TLR/MyD88/NF-κB signaling pathway. Additionally, HY7718 reduced the blood levels of pro-inflammatory cytokines, as well as reversing DSS-induced changes to the composition of the intestinal microbiome. HY7718 also increased the percentage of beneficial bacteria (Lactiplantibacillus and Bifidobacterium), which correlated positively with the expression of intestinal TJ-related genes. Finally, HY7718 decreased the population of pathogens such as Escherichia, which correlated with IBD symptoms. The data suggest that HY7718 improves intestinal integrity in colitis model mice by regulating the expression of TJ proteins and inflammatory cytokines, as well as the composition of the intestinal microflora. Thus, L. plantarum HY7718 may be suitable as a functional supplement that improves IBD symptoms and gut health.
Traces of Canine Inflammatory Bowel Disease Reflected by Intestinal Organoids
Authors: Pratscher Barbara , Kuropka Benno , Csukovich Georg , Doulidis Pavlos G. , Spirk Katrin , Kramer Nina , Freund Patricia , Rodríguez-Rojas Alexandro , Burgener Iwan A.
Published: January 2024

Inflammatory bowel disease (IBD) is a chronic inflammatory condition that affects humans and several domestic animal species, including cats and dogs. In this study, we have analyzed duodenal organoids derived from canine IBD patients using quantitative proteomics. Our objective was to investigate whether these organoids show phenotypic traits of the disease compared with control organoids obtained from healthy donors. To this aim, IBD and control organoids were subjected to quantitative proteomics analysis via liquid chromatography–mass spectrometry. The obtained data revealed notable differences between the two groups. The IBD organoids exhibited several alterations at the levels of multiple proteins that are consistent with some known IBD alterations. The observed phenotype in the IBD organoids to some degree mirrors the corresponding intestinal condition, rendering them a compelling approach for investigating the disease and advancing drug exploration. Additionally, our study revealed similarities to some human IBD biomarkers, further emphasizing the translational and comparative value of dogs for future investigations related to the causes and treatment of IBD. Relevant proteins such as CALU, FLNA, MSN and HMGA2, which are related to intestinal diseases, were all upregulated in the IBD duodenal organoids. At the same time, other proteins such as intestinal keratins and the mucosal immunity PIGR were depleted in these IBD organoids. Based on these findings, we propose that these organoids could serve as a valuable tool for evaluating the efficacy of therapeutic interventions against canine IBD.
Regulators of G-Protein Signaling (RGS) in Sporadic and Colitis-Associated Colorectal Cancer
Authors: Swierczynski Mikolaj , Kasprzak Zuzanna , Makaro Adam , Salaga Maciej
Published: January 2024

Colorectal cancer (CRC) is one of the most common neoplasms worldwide. Among the risk factors of CRC, inflammatory bowel disease (IBD) is one of the most important ones leading to the development of colitis-associated CRC (CAC). G-protein coupled receptors (GPCR) are transmembrane receptors that orchestrate a multitude of signaling cascades in response to external stimuli. Because of their functionality, they are promising targets in research on new strategies for CRC diagnostics and treatment. Recently, regulators of G-proteins (RGS) have been attracting attention in the field of oncology. Typically, they serve as negative regulators of GPCR responses to both physiological stimuli and medications. RGS activity can lead to both beneficial and harmful effects depending on the nature of the stimulus. However, the atypical RGS—AXIN uses its RGS domain to antagonize key signaling pathways in CRC development through the stabilization of the β-catenin destruction complex. Since AXIN does not limit the efficiency of medications, it seems to be an even more promising pharmacological target in CRC treatment. In this review, we discuss the current state of knowledge on RGS significance in sporadic CRC and CAC with particular emphasis on the regulation of GPCR involved in IBD-related inflammation comprising opioid, cannabinoid and serotonin receptors.
Astrocytic Regulation of Endocannabinoid-Dependent Synaptic Plasticity in the Dorsolateral Striatum
Authors: Adermark Louise , Stomberg Rosita , Söderpalm Bo , Ericson Mia
Published: January 2024

Astrocytes are pivotal for synaptic transmission and may also play a role in the induction and expression of synaptic plasticity, including endocannabinoid-mediated long-term depression (eCB-LTD). In the dorsolateral striatum (DLS), eCB signaling plays a major role in balancing excitation and inhibition and promoting habitual learning. The aim of this study was to outline the role of astrocytes in regulating eCB signaling in the DLS. To this end, we employed electrophysiological slice recordings combined with metabolic, chemogenetic and pharmacological approaches in an attempt to selectively suppress astrocyte function. High-frequency stimulation induced eCB-mediated LTD (HFS-LTD) in brain slices from both male and female rats. The metabolic uncoupler fluorocitrate (FC) reduced the probability of transmitter release and depressed synaptic output in a manner that was independent on cannabinoid 1 receptor (CB1R) activation. Fluorocitrate did not affect the LTD induced by the CB1R agonist WIN55,212-2, but enhanced CB1R-dependent HFS-LTD. Reduced neurotransmission and facilitated HFS-LTD were also observed during chemogenetic manipulation using Gi-coupled DREADDs targeting glial fibrillary acidic protein (GFAP)-expressing cells, during the pharmacological inhibition of connexins using carbenoxolone disodium, or during astrocytic glutamate uptake using TFB-TBOA. While pretreatment with the N-methyl-D-aspartate (NMDA) receptor antagonist 2-amino-5-phosphonopentanoic acid (APV) failed to prevent synaptic depression induced by FC, it blocked the facilitation of HFS-LTD. While the lack of tools to disentangle astrocytes from neurons is a major limitation of this study, our data collectively support a role for astrocytes in modulating basal neurotransmission and eCB-mediated synaptic plasticity.
Multi-Target-Directed Cinnamic Acid Hybrids Targeting Alzheimer’s Disease
Authors: Drakontaeidi Aliki , Pontiki Eleni
Published: January 2024

Progressive cognitive decline in Alzheimer’s disease (AD) is a growing challenge. Present therapies are based on acetylcholinesterase inhibition providing only temporary relief. Promising alternatives include butyrylcholinesterase (BuChE) inhibitors, multi-target ligands (MTDLs) that address the multi-factorial nature of AD, and compounds that target oxidative stress and inflammation. Cinnamate derivatives, known for their neuroprotective properties, show potential when combined with established AD agents, demonstrating improved efficacy. They are being positioned as potential AD therapeutic leads due to their ability to inhibit Aβ accumulation and provide neuroprotection. This article highlights the remarkable potential of cinnamic acid as a basic structure that is easily adaptable and combinable to different active groups in the struggle against Alzheimer’s disease. Compounds with a methoxy substitution at the para-position of cinnamic acid display increased efficacy, whereas electron-withdrawing groups are generally more effective. The effect of the molecular volume is worthy of further investigation.