bluuebunny/crossref_metadata_2025 huggingface dataset:
Revision of the subgenus Phortica (sensu stricto) (Diptera, Drosophilidae) from East Asia, with assessment of species delimitation using DNA barcodes
Authors: HUANG JIA , GONG LU , TSAUR SHUN-CHERN , ZHU LIN , AN KEYING , CHEN HONGWEI
Published: September 2019

A total of 50 (43 known and seven new) species in the subgenus Phortica (sensu stricto) were surveyed and (re)described from China: P. bicornuta (Chen & Toda, 1997); P. bipartita (Toda & Peng, 1992); P. biprotrusa (Chen & Toda, 1998); P. cardua (Okada, 1977); P. chi (Toda & Sidorenko, 1996); P. conifera (Okada, 1977); P. eparmata (Okada, 1977); P. eugamma (Toda & Peng, 1990); P. excrescentiosa (Toda & Peng, 1990); P. fangae (Máca, 1993); P. flexuosa (Zhang & Gan, 1986); P. foliata (Chen & Toda, 1997); P. gamma (Toda & Peng, 1990); P. gigas (Okada, 1977); P. glabtabula Chen & Gao, 2005; P. hainanensis (Chen & Toda, 1998); P. hongae (Máca, 1993); P. huazhii Cheng & Chen, 2008; P. iota (Toda & Sidorenko, 1996); P. jadete Zhu, Cao & Chen, 2018; P. kappa (Máca, 1977); P. lambda (Toda & Peng, 1990); P. latifoliacea Chen & Watabe, 2008; P. magna (Okada, 1960); P. okadai (Máca, 1977); P. omega (Okada, 1977); P. orientalis (Hendel, 1914); P. pangi Chen & Wen, 2005; P. paramagna (Okada, 1971); P. perforcipata (Máca & Lin, 1993); P. pi (Toda & Peng, 1990); P. protrusa (Zhang & Shi, 1997); P. pseudopi (Toda & Peng, 1990); P. pseudotau (Toda & Peng, 1990); P. psi (Zhang & Gan, 1986); P. rhagolobos Chen & Gao, 2008; P. saeta (Zhang & Gan, 1986); P. setitabula Chen & Gao, 2005; P. subradiata (Okada, 1977); P. tau (Toda & Peng, 1990); P. uncinata Chen & Gao, 2005; P. unipetala Chen & Wen, 2005; P. allomega Gong & Chen, sp. nov.; P. archikappa Gong & Chen, sp. nov.; P. dianzangensis Gong & Chen, sp. nov.; P. imbacilia Gong & Chen, sp. nov.; P. liukuni Gong & Chen, sp. nov.; P. tibeta Gong & Chen, sp. nov.; and P. xianfui Gong & Chen, sp. nov. In addition, seven new synonyms were recognized: P. acongruens (Zhang & Shi, 1997), syn. nov.; P. antillaria (Chen & Toda, 1997), syn. nov.; P. kukuanensis Máca, 2003, syn. nov.; P. linae (Máca & Chen, 1993), syn. nov.; P. shillongensis (Singh & Gupta, 1979), syn. nov.; P. takadai (Okada, 1977), syn. nov.; and P. watanabei (Máca & Lin, 1993), syn. nov. A key to all Asian species (except for the eparmata species complex) of this subgenus was provided. All currently available DNA barcode (partial mitochondrial cytochrome c oxidase subunit I (COI) gene) sequences of this subgenus (217 sequences of 54 species) are employed in a molecular analysis using different species delimitation methods. The results indicate that approximately 68.5% (37 of 54 spp.) of Phortica (s. str.) species could be clearly distinguished from closely related morphospecies or cryptic species.
The relationship of the shear wave elastography findings of patients with unilateral lumbar disc herniation and clinical characteristics
Authors: Wang Qingyuan , Zhang Hao , Zhang Jinxin , Zhang Hanqi , Zheng Hui
Published: September 2019

Background

The aim of this study was to find out whether the shear wave elastography (SWE) findings of patients with unilateral lumbar disc herniation (LDH) were related to clinical characteristics.

Methods

For this purpose, the study group included patients (N = 20; 13 male, 7 female) with complaints of unilateral sciatica, with foraminal stenosis caused by one level of LDH (L4-L5 or L5-S1). An gender-and age-matched control group (N = 27; 16 male, 11 female) was included. All the patients were examined on both the axial and longitudinal planes bilaterally at the same level using a convex array probe (1- 6 MHz, Supersonic Imagine, Aix en Provence, France).

Results

The sciatic nerve stiffness measured on longitudinal planes of the affected side was significantly higher than unaffected side (p < 0.001) and the control group (P < 0.05). Furthermore, the symptom duration of unilateral LDH is positively correlated with the stiffness the sciatic nerve (r = 0.52, p = 0.019).

Conclusion

According to these findings, ultrasound imaging can be considered as a useful tool to detect changes in the sciatic nerve due to disc herniation. This technique will have a promising prospect for many patients with unilateral LDH in monitoring stiffness during rehabilitation and before or after surgery.
Effect of LongRange™ eprinomectin on Anopheles arabiensis by feeding on calves treated with the drug
Authors: Belay Aklilu , Petros Beyene , Gebre-Michael Teshome , Balkew Meshesha
Published: September 2019

Background

Misuse of long-lasting insecticidal nets together with resistance of vectors to most of the insecticides for indoor residual spraying and impregnated nets threaten malaria vector control interventions, requiring search for alternative control methods. Reports have shown that Anopheles mosquitoes die when they feed on endectocidal drugs used to treat humans and animals. A study was designed to investigate the efficacy of LongRange™ (eprinomectin 5%) on laboratory reared Anopheles arabiensis fed on treated calves.

Methods

Anopheles arabiensis from insectary colony was fed on three calves treated with therapeutic dose of LongRange™ eprinomectin (1 ml/50 kg) and on non-treated three other calves as control arm. For the feeding, mosquitoes were placed in paper cups covered with nylon cloth mesh and then allowed to feed on the necks of calves. Subsequently, mosquito survival, fecundity, egg hatchability, larval development and adult emergence were recorded. Data were entered and analysed by using SPSS version 20. The Kaplan–Meier survival analysis and independent sample t-test were used.

Results

All mosquitoes that fed on LongRange™ Eprinomectin treated calves died within 7 days following blood ingestion. The drug also slightly affected fecundity and hatchability of An. arabiensis.

Conclusion

Treating livestock with LongRange™ (eprinomectin 5%) may serve as a supplementary control method for zoophagic An. arabiensis.
Milk-derived exosomes (MDEs) have a different biological effect on normal fetal colon epithelial cells compared to colon tumor cells in a miRNA-dependent manner
Authors: Reif Shimon , Elbaum Shiff Yaffa , Golan-Gerstl Regina
Published: September 2019

Background

Breastfeeding is the ideal source of infant nutrition. Human milk consists not only of nutrients but also biologically active components. Among these latter compounds, exosomes contain proteins, lipids, mRNAs and miRNAs.

Methods

To elucidate the biological effects of milk-derived exosomes (MDEs) on normal colonic epithelial cells compared to colonic tumor cells, we incubated cells with MDEs. MDEs were able to enter into normal and tumor cells and change their miRNA expression profiles. Proliferation, cell morphology and protein expression were analyzed in these cells.

Results

Human milk-derived exosomes induced proliferation- and epithelial mesenchymal transformation-related changes, such as collagen type I and twist expression, in normal but not in tumor cells. PTEN, a target of miRNA-148a, was downregulated in normal but not in tumor cells following incubation with MDEs. Moreover, miRNA-148a-3p knockdown cells were used to demonstrate the importance of miRNA in the effect of exosomes on cell proliferation and protein expression. MDEs inhibited proliferation and DNMT1 expression in cells with knockdown of miRNA-148a.

Conclusions

In conclusion, the positive effect of exosomes on normal cells without affecting tumor cells may presents an aspect of their safety when considering it use as a nutritional supplement to infant formula.
Uncertainty quantification of parenchymal tracer distribution using random diffusion and convective velocity fields
Authors: Croci Matteo , Vinje Vegard , Rognes Marie E.
Published: September 2019

Background

Influx and clearance of substances in the brain parenchyma occur by a combination of diffusion and convection, but the relative importance of these mechanisms is unclear. Accurate modeling of tracer distributions in the brain relies on parameters that are partially unknown and with literature values varying by several orders of magnitude. In this work, we rigorously quantified the variability of tracer distribution in the brain resulting from uncertainty in diffusion and convection model parameters.

Methods

Using the convection–diffusion–reaction equation, we simulated tracer distribution in the brain parenchyma after intrathecal injection. Several models were tested to assess the uncertainty both in type of diffusion and velocity fields and also the importance of their magnitude. Our results were compared with experimental MRI results of tracer enhancement.

Results

In models of pure diffusion, the expected amount of tracer in the gray matter reached peak value after 15 h, while the white matter did not reach peak within 24 h with high likelihood. Models of the glymphatic system were similar qualitatively to the models of pure diffusion with respect to expected time to peak but displayed less variability. However, the expected time to peak was reduced to 11 h when an additional directionality was prescribed for the glymphatic circulation. In a model including drainage directly from the brain parenchyma, time to peak occured after 6–8 h for the gray matter.

Conclusion

Even when uncertainties are taken into account, we find that diffusion alone is not sufficient to explain transport of tracer deep into the white matter as seen in experimental data. A glymphatic velocity field may increase transport if a large-scale directional structure is included in the glymphatic circulation.
Functional polysaccharides of carob fruit: a review
Authors: Zhu Bao-Jie , Zayed Mohamed Zaky , Zhu Hua-Xu , Zhao Jing , Li Shao-Ping
Published: September 2019

Polysaccharides in carob fruit, including carob bean gum (also known as carob gum, locust bean gum) and carob fiber, are widely used in industries such as food, pharmaceuticals, paper, textile, oil well drilling and cosmetics. Carob bean gum is a galactomannan obtained from the seed endosperm of carob tree and the fiber is obtained by removing most of soluble carbohydrates in carob pulp by water extraction. Both the gum and fiber are beneficial to health for many diseases such as diabetes, bowel movements, heart disease and colon cancer. This article reviewed the composition, properties, food applications and health benefits of polysaccharides from carob fruit.
Specific DNA identification of Pheretima in the Naoxintong capsule
Authors: Zhu Xiaoxiao , Wu Hoi-Yan , Shaw Pang-Chui , Peng Wei , Su Weiwei
Published: September 2019

Background

Pheretima is a minister drug in Naoxintong capsule (NXTC), a well-known traditional Chinese medicine (TCM) formula for the treatment of cardiovascular and cerebrovascular diseases. Owing to the loss of morphological and microscopic characteristics and the lack of recognized chemical marker, it is difficult to identify Pheretima in NXTC. This study aims to evaluate the feasibility of using DNA techniques to authenticate Pheretima, especially when it is processed into NXTC.

Methods

DNA was extracted from crude drugs of the genuine and adulterant species, as well as nine batches of NXTCs. Based on mitochondrial cytochrome c oxidase subunit I (COI) gene, specific primers were designed for two genera of genuine species, Metaphire and Amynthas, respectively. PCR amplification was performed with the designed primers on crude drugs of Pheretima and NXTCs. The purified PCR products were sequenced and the obtained sequences were identified to species level with top hit of similarity with BLAST against GenBank nucleotide database.

Results

Primers MF2R2 and AF3R1 could amplify specific DNA fragments with sizes around 230–250 bp, both in crude drugs and NXTC. With sequencing and the BLAST search, identities of the tested samples were found.

Conclusion

This study indicated that the molecular approach is effective for identifying Pheretima in NXTC. Therefore, DNA identification may contribute to the quality control and assurance of NXTC.
Discovery and implementation of a novel pathway for n-butanol production via 2-oxoglutarate
Authors: Ferreira Sofia , Pereira Rui , Liu Filipe , Vilaça Paulo , Rocha Isabel
Published: September 2019

Background

One of the European Union directives indicates that 10% of all fuels must be bio-synthesized by 2020. In this regard, biobutanol—natively produced by clostridial strains—poses as a promising alternative biofuel. One possible approach to overcome the difficulties of the industrial exploration of the native producers is the expression of more suitable pathways in robust microorganisms such as Escherichia coli. The enumeration of novel pathways is a powerful tool, allowing to identify non-obvious combinations of enzymes to produce a target compound.

Results

This work describes the in silico driven design of E. coli strains able to produce butanol via 2-oxoglutarate by a novel pathway. This butanol pathway was generated by a hypergraph algorithm and selected from an initial set of 105,954 different routes by successively applying different filters, such as stoichiometric feasibility, size and novelty. The implementation of this pathway involved seven catalytic steps and required the insertion of nine heterologous genes from various sources in E. coli distributed in three plasmids. Expressing butanol genes in E. coli K12 and cultivation in High-Density Medium formulation seem to favor butanol accumulation via the 2-oxoglutarate pathway. The maximum butanol titer obtained was 85 ± 1 mg L−1 by cultivating the cells in bioreactors.

Conclusions

In this work, we were able to successfully translate the computational analysis into in vivo applications, designing novel strains of E. coli able to produce n-butanol via an innovative pathway. Our results demonstrate that enumeration algorithms can broad the spectrum of butanol producing pathways. This validation encourages further research to other target compounds.
Identifying Crohn’s disease signal from variome analysis
Authors: Wang Yanran , Miller Maximilian , Astrakhan Yuri , Petersen Britt-Sabina , Schreiber Stefan , Franke Andre , Bromberg Yana
Published: September 2019

Background

After years of concentrated research efforts, the exact cause of Crohn’s disease (CD) remains unknown. Its accurate diagnosis, however, helps in management and preventing the onset of disease. Genome-wide association studies have identified 241 CD loci, but these carry small log odds ratios and are thus diagnostically uninformative.

Methods

Here, we describe a machine learning method—AVA,Dx (Analysis of Variation for Association with Disease)—that uses exonic variants from whole exome or genome sequencing data to extract CD signal and predict CD status. Using the person-specific coding variation in genes from a panel of only 111 individuals, we built disease-prediction models informative of previously undiscovered disease genes. By additionally accounting for batch effects, we were able to accurately predict CD status for thousands of previously unseen individuals from other panels.

Results

AVA,Dx highlighted known CD genes including NOD2 and new potential CD genes. AVA,Dx identified 16% (at strict cutoff) of CD patients at 99% precision and 58% of the patients (at default cutoff) with 82% precision in over 3000 individuals from separately sequenced panels.

Conclusions

Larger training panels and additional features, including other types of genetic variants and environmental factors, e.g., human-associated microbiota, may improve model performance. However, the results presented here already position AVA,Dx as both an effective method for revealing pathogenesis pathways and as a CD risk analysis tool, which can improve clinical diagnostic time and accuracy. Links to the AVA,Dx Docker image and the BitBucket source code are at https://bromberglab.org/project/avadx/.
microRNA-92a regulates the expression of aphid bacteriocyte-specific secreted protein 1
Authors: Feng Honglin , Park Joun S. , Zhai R. Grace , Wilson Alexandra C. C.
Published: September 2019

Objective

Aphids harbor a nutritional obligate endosymbiont in specialized cells called bacteriocytes, which aggregate to form an organ known as the bacteriome. Aphid bacteriomes display distinct gene expression profiles that facilitate the symbiotic relationship. Currently, the mechanisms that regulate these patterns of gene expression are unknown. Recently using computational pipelines, we identified miRNAs that are conserved in expression in the bacteriomes of two aphid species and proposed that they function as important regulators of bacteriocyte gene expression. Here using a dual luciferase assay in mouse NIH/3T3 cell culture, we aimed to experimentally validate the computationally predicted interaction between Myzus persicae miR-92a and the predicted target region of M. persicae bacteriocyte-specific secreted protein 1 (SP1) mRNA.

Results

In the dual luciferase assay, miR-92a interacted with the SP1 target region resulting in a significant downregulation of the luciferase signal. Our results demonstrate that miR-92a interacts with SP1 to alter expression in a heterologous expression system, thereby supporting our earlier assertion that miRNAs are regulators of the aphid/Buchnera symbiotic interaction.
Malignant mesothelioma in construction workers: the Apulia regional mesothelioma register, Southern Italy
Authors: Vimercati L. , Cavone D. , Caputi A. , Delfino M. C. , De Maria L. , Ferri G. M. , Serio G.
Published: September 2019

Objective

Asbestos was widely used in construction in both a friable and a compact form until the 1990s, before its use was banned. Today, many of these materials are still in situ and represent a source of risk for construction workers. The objective of the study was to analyse the cases of mesothelioma arising among construction workers registered in the Apulia regional register of mesothelioma.

Results

For the period 1993–2018, there were 178 male cases, and 10.2% of the cases were present in the regional register. The average age at diagnosis was 64.7 years. The site was pleural in 96.06% of cases, with a diagnosis of certainty in 86.5% of the total cases and 61.8% of cases with epithelial histology. The average latency is 43.9 years. In 75.2% of cases, the exposure began between 1941 and 1970, with an average duration of 24.3 years. The age at the start of exposure in 68.5% of cases is between 8 and 20 years. The ORs were 2.5 (C.I. 95% 1.01–6.17) for the epithelioid histotype and the high duration of exposure. The data underline the need for prevention and information on all activities involving construction workers in which asbestos-containing materials are still used.
Breast self-examination practice and associated factors among female healthcare workers in West Shoa Zone, Western Ethiopia 2019: a cross-sectional study
Authors: Shallo Seifadin Ahmed , Boru Jibril Dori
Published: September 2019

Objective

Breast cancer is the leading cause of death among reproductive-age women worldwide and the second leading cause of death among women in Ethiopia. Regular breast self-examination is the most cost-effective methods for early detection of breast cancer. Despite this fact, breast self-examination was low among women in the general population and it was not well documented among health care workers. Therefore, this study intended to assess the magnitude of breast self-examination practice and associated factors among female healthcare workers in public health facility in West Shoa Zone, Ethiopia. Institutional based cross-sectional study was conducted among 379 female healthcare workers. The study participants were recruited by lottery method. Data were collected from March to April 2019. Data were entered into Epi data version 4.5 and analyzed using SPSS version 25. Bivariate and multiple logistic regressions analysis were done. With 95% CI, the level of significance was decided at P-value ≤ 0.05.

Results

The magnitude of regular breast self-examination practice was 32.6%. Educational levels, breast cancer awareness, knowledge towards breast self-examination were predictors of breast self-examination. Regular awareness creation forum on breast self-examination technique, risk factors, and related matters should be facilitated so that all female health care workers will be reminded.
Morpho-functional evaluation of lung aeration as a marker of sickle-cell acute chest syndrome severity in the ICU: a prospective cohort study
Authors: Garnier Marc , Hafiani El Mahdi , Arbelot Charlotte , Blayau Clarisse , Labbe Vincent , Stankovic-Stojanovic Katia , Lionnet François , Bonnet Francis , Fulgencio Jean-Pierre , Fartoukh Muriel , Quesnel Christophe
Published: September 2019

Background

Acute chest syndrome (ACS) is the main cause of morbi-mortality in patients with sickle-cell disease in the intensive care unit (ICU). ACS definition encompasses many types of lung damage, making early detection of the most severe forms challenging. We aimed to describe ACS-related lung ultrasound (LU) patterns and determine LU performance to assess ACS outcome.

Results

We performed a prospective cohort study including 56 ICU patients hospitalized for ACS in a tertiary university hospital (Paris, France). LU and bedside spirometry were performed at admission (D0) and after 48 h (D2). Complicated outcome was defined by the need for transfusion of ≥ 3 red blood cell units, mechanical ventilation, ICU length-of-stay > 5 days, or death. A severe loss of lung aeration was observed in all patients, predominantly in inferior lobes, and was associated with decreased vital capacity (22 [15–33]% of predicted). The LU Score was 24 [20–28] on D0 and 20 [15–24] on D2. Twenty-five percent of patients (14/56) had a complicated outcome. Neither oxygen supply, pain score, haemoglobin, LDH and bilirubin values at D0; nor their change at D2, differed regarding patient outcome. Conversely, LU re-aeration score and spirometry change at D2 improved significantly more in patients with a favourable outcome. A negative LU re-aeration score at D2 was an independent marker of severity of ACS in ICU.

Conclusions

ACS is associated with severe loss of lung aeration, whose resolution is associated with favourable outcome. Serial bedside LU may accurately and early identify ACS patients at risk of complicated outcome.
Early prediction of noninvasive ventilation failure in COPD patients: derivation, internal validation, and external validation of a simple risk score
Authors: Duan Jun , Wang Shengyu , Liu Ping , Han Xiaoli , Tian Yao , Gao Fan , Zhou Jing , Mou Junhuan , Qin Qian , Yu Jingrong , Bai Linfu , Zhou Lintong , Zhang Rui
Published: September 2019

Background

Early identification of noninvasive ventilation (NIV) failure is a promising strategy for reducing mortality in chronic obstructive pulmonary disease (COPD) patients. However, a risk-scoring system is lacking.

Methods

To develop a scale to predict NIV failure, 500 COPD patients were enrolled in a derivation cohort. Heart rate, acidosis (assessed by pH), consciousness (assessed by Glasgow coma score), oxygenation, and respiratory rate (HACOR) were entered into the scoring system. Another two groups of 323 and 395 patients were enrolled to internally and externally validate the scale, respectively. NIV failure was defined as intubation or death during NIV.

Results

Using HACOR score collected at 1–2 h of NIV to predict NIV failure, the area under the receiver operating characteristic curves (AUC) was 0.90, 0.89, and 0.71 for the derivation, internal-validation, and external-validation cohorts, respectively. For the prediction of early NIV failure in these three cohorts, the AUC was 0.91, 0.96, and 0.83, respectively. In all patients with HACOR score > 5, the NIV failure rate was 50.2%. In these patients, early intubation (< 48 h) was associated with decreased hospital mortality (unadjusted odds ratio = 0.15, 95% confidence interval 0.05–0.39,p < 0.01).

Conclusions

HACOR scores exhibited good predictive power for NIV failure in COPD patients, particularly for the prediction of early NIV failure (< 48 h). In high-risk patients, early intubation was associated with decreased hospital mortality.
A New 3D Empirical Plastic and Damage Model for Simulating the Failure of Concrete Structure
Authors: Jiao Yan-tao , Wang Bo , Shen Zhen-zhong
Published: September 2019

A new plastic–damage constitutive model based on the combination of damage mechanics and classical plastic theory was developed to simulate the failure of concrete. In order to explain different material behaviors of concrete under tensile and compressive loadings, the plastic yield criterion, the different kinematic hardening rule for tension and compressive and the isotropic flow rule were established in the effective stress space. Meanwhile, two different empirical damage evolution equations were adopted: one for compression and the other for tension. A multi-axial damage influence factor was also introduced to fully describe the anisotropic damage of concrete. Finally, the model response was compared with a wide range of experiment results. The results showed that the model could well describe the nonlinear behavior of concrete in a complex stress state.
Micro-invasive glaucoma surgery – an interventional glaucoma revolution
Authors: Shah Manjool
Published: September 2019

The glaucoma surgical landscape has changed dramatically over the last decade with the introduction and integration of micro-invasive glaucoma surgery (MIGS) techniques. These modalities target physiologic outflow pathways or optimize previously utilized glaucoma surgical methods in order to deliver safety, efficacy, and individualized care to the patient. MIGS techniques can be classified based on anatomical location as well as method of intraocular pressure (IOP) reduction. This review will focus on MIGS optimizing the conventional outflow pathway via intervention at Schlemm’s canal, MIGS optimizing the uveoscleral outflow pathway via suprachoroidal shunting, and MIGS optimizing the transscleral or subconjunctival outflow pathway which has long been utilized by glaucoma surgeons performing traditional filtration procedures. The wide array of currently available MIGS modalities can be staggering to the glaucoma care provider, but an understanding of the landscape and the large classes of interventional strategies can allow for clinical decision making based on the specifics of the patient’s needs and the pathophysiology of their disease.
Draft genome sequence of fastidious pathogen Ceratobasidium theobromae, which causes vascular-streak dieback in Theobroma cacao
Authors: Ali Shahin S. , Asman Asman , Shao Jonathan , Firmansyah Amanda P. , Susilo Agung W. , Rosmana Ade , McMahon Peter , Junaid Muhammad , Guest David , Kheng Tee Yei , Meinhardt Lyndel W. , Bailey Bryan A.
Published: September 2019

Background

Ceratobasidium theobromae, a member of the Ceratobasidiaceae family, is the causal agent of vascular-streak dieback (VSD) of cacao, a major threat to the chocolate industry in the South-East Asia. The fastidious pathogen is very hard to isolate and maintain in pure culture, which is a major bottleneck in the study of its genetic diversity and genome.

Result

This study describes for the first time, a 33.90 Mbp de novo assembled genome of a putative C. theobromae isolate from cacao. Ab initio gene prediction identified 9264 protein-coding genes, of which 800 are unique to C. theobromae when compared to Rhizoctonia spp., a closely related group. Transcriptome analysis using RNA isolated from 4 independent VSD symptomatic cacao stems identified 3550 transcriptionally active genes when compared to the assembled C. theobromae genome while transcripts for only 4 C. theobromae genes were detected in 2 asymptomatic stems. De novo assembly of the non-cacao associated reads from the VSD symptomatic stems uniformly produced genes with high identity to predicted genes in the C. theobromae genome as compared to Rhizoctonia spp. or genes found in Genbank. Further analysis of the predicted C. theobromae transcriptome was carried out identifying CAZy gene classes, KEGG-pathway associated genes, and 138 putative effector proteins.

Conclusion

These findings put forth, for the first time, a predicted genome for the fastidious basidiomycete C. theobromae causing VSD on cacao providing a model for testing and comparison in the future. The C. theobromae genome predicts a pathogenesis model involving secreted effector proteins to suppress plant defense mechanisms and plant cell wall degrading enzymes.
Patients’ appreciation of pre-implant augmentation of the severely resorbed maxilla with calvarial or anterior iliac crest bone:a randomized controlled trial
Authors: Wortmann Dagmar E. , Boven Carina G. , Schortinghuis Jurjen , Vissink Arjan , Raghoebar Gerry M.
Published: September 2019

Background

Little is known about the impact of bone graft harvesting for pre-implant augmentation of the maxilla from a patient’s perspective. To assess patient-reported outcome measures (PROMs) related to augmentation of the extremely resorbed edentulous maxilla with calvarial or anterior iliac crest bone.

Materials and methods

For this randomised controlled trial, 20 consecutive edentulous patients needing extensive pre-implant surgery of the maxilla were randomly assigned to either calvarial (n = 10) or anterior iliac crest (n = 10) bone harvesting. Patient reports on procedure-related satisfaction, questionnaires on oral functionality (denture satisfaction, chewing ability) and oral health-related quality of life (OHIP-49NL) and subjective donor site-related outcomes (e.g. of post-operative pain, scar formation, physical mobility) were assessed.

Results

Irrespective of the harvesting site, patients were generally satisfied (median VAS score 93 (86–99) mm, p = 0.400) with the procedure and its final results. Post-operative pain was mild (median 40 (20–40) mm) and decreased to no pain (4 (0–16) mm) within 14 days. Early post-operative pain was significantly higher following anterior iliac crest harvesting (p < 0.00). Impact on physical mobility, daily functioning and satisfaction with the scar formation were similar in both groups.

Conclusions

The assessed PROMs confirmed that bone graft harvesting from the calvarium or anterior iliac crest is an appropriate procedure, reflected by high levels of satisfaction, minor long-term sequela and improvement of perceived oral health. For clinical decision-making, decisions can be based on individual features and preferences.

Trial registration

NTR, NTR3968, registered 1 July 2013.
Prevalence of focal incidental breast uptake on FDG-PET/CT and risk of malignancy: a systematic review and meta-analysis
Authors: Aarstad Else Marie , Nordhaug Petter , Naghavi-Behzad Mohammad , Larsen Lisbet Brønsro , Gerke Oke , Hildebrandt Malene Grubbe
Published: September 2019

Background

FDG-PET/CT is increasingly used for oncologic and inflammatory diseases. Focal incidental FDG uptake occurs rarely in breast tissue but has often significant consequences. This study aimed to systematically review literature regarding focal incidental breast uptake (FIBU) on FDG-PET/CT in order to yield an update on the prevalence and risk of malignancy for FIBU.

Methods

A systematic search for relevant articles published between 2012 and 2018 was performed through MEDLINE, Embase, and Cochrane databases. Studies addressing the detection of FIBU in patients without a previous history of breast malignancy were included. The QUADAS-2 was used for quality assessment, and eligible data were pooled using a fixed-effects model.I2was calculated for the heterogeneity between studies.

Results

Eight studies containing 180,002 scans were included in the systematic review. The median prevalence of FIBU for both genders was 0.52% (range 0.18–22.5%). Prevalence for women was mentioned separately in five studies and varied from 0.51 to 23.5%. One study reporting a high prevalence was based on patients being staged for known malignancy, and the word “breast” was used in the search, which may have caused selection bias. Data from four studies were eligible for meta-analysis. A high degree of heterogeneity was observed for prevalence data (I2of 97.5%), while moderate heterogeneity was observed for data on malignancy risk assessment (I2of 62.8%). The pooled prevalence of FIBU in women was 0.61% (range 0.56–0.66%), and the pooled prevalence of malignancy of FIBUs was 38.7% (range 34.4–43.0%). The most commonly detected malignancy was invasive ductal carcinoma.

Conclusion

FIBU occurs rarely on FDG-PET/CT for female patients but yields a high risk of malignancy according to the results of published papers. Therefore, it should be considered relevant to further elucidate patients with incidentally detected FDG uptake in breast in clinical practice.
Network like a demon!
Authors: Faulkner Clare
Published: September 2019

Clare Faulkner offers some great reasons to attend dental conferences and shows and get up close and personal to what it is that ticks your boxes
Professional and personal conduct – what's expected?
Authors: Collier Andrew
Published: September 2019

Dental nurse registration with the General Dental Council gives rights and privileges but also responsibilities and obligations. Andrew Collier shines a light on what this means for the dental nurse

Aim

To describe the ethical and legal obligations of a registered dental nurse and inform where to seek guidance regarding those obligations

Objectives

To have an enhanced understanding of a dental nurse's professional role and responsibilities when treating patients To have an enhanced understanding of a dental nurse's professional responsibilities outside of the workplace To understand the dental nurse's Continuing Professional Development (CPD) obligations and where to seek further guidance To understand the dental nurse's indemnity/insurance obligations and where to seek further guidance GDC development outcomes A and D
Achieve what you believe
Authors: Meldrum Eloise
Published: September 2019

Dental nurse Eloise Meldrum recently changed jobs. Here, she shares how we can take that leap of faith
Everything to play for
Authors: Hingston Rachel , Campbell-Barr Verity
Published: September 2019

Do children value learning as much as play? Rachel Hingston conducts interviews and observes children's behaviour in their learning environment to discover whether play always trumps learning.
Upcycle old books
Authors: White Hilary
Published: September 2019

In part four of her series on sustainability, Hilary White explores the different ways we can reuse materials such as picture books to teach children the value of breathing new life into old objects.
Editorial: Knowledge is Power, Ignorance is Bliss: Public perceptions and responses to human trafficking
Authors: Sharapov Kiril , Hoff Suzanne , Gerasimov Borislav
Published: September 2019

The focus of this issue of the Anti-Trafficking Review—public perceptions and responses to human trafficking—reflects the growing unease and disagreements among anti-trafficking practitioners and scholars about the current state of public awareness of human trafficking: how and by whom such awareness is produced and manipulated, whom it is targeting, and whether it leads, or can lead, to any meaningful anti-trafficking action. A central assumption in the anti-trafficking field is that the general public still lacks sufficient knowledge about human trafficking, and that creating more knowledge and awareness will lead to its reduction. However, there neither exists a common understanding of who should know what in order to achieve this goal, nor is there sufficient information available about the awareness of the general public or, especially, the impact of this awareness.
Public Understanding of Trafficking in Human Beings in Great Britain, Hungary and Ukraine
Authors: Sharapov Kiril
Published: September 2019

This article provides a summary of research undertaken to investigate public awareness and understanding of human trafficking in Great Britain, Hungary and Ukraine. Responding to the lack of reliable empirical data on this issue, the research relies on representative national opinion surveys to assess the extent of public awareness of what constitutes human trafficking, the sources of knowledge underpinning this awareness, and respondents’ attitudes towards key dimensions of human trafficking as embedded in international and respective national legal and policy frameworks and discourses. Conceptually, this article reinforces recent calls for policy and media paradigm shifts from understanding human trafficking as a phenomenon of crime and victimhood, to, above all, a human rights concern linked to the broader issues of sustainable development and social justice. Methodologically, the study highlights the role of opinion surveys as a measure of effectiveness and impact of anti-trafficking awareness campaigns. In practical terms, the article presents a set of data which can be useful for policy-makers, anti-trafficking activists, and national media in designing impactful awareness-raising campaigns and interventions.
Proteomics analysis of serum protein patterns in duck during aflatoxin B1 exposure
Authors: Tansakul Natthasit , Rattanasrisomporn Jatuporn , Roytrakul Sittiruk
Published: September 2019

Background and Aim: Unlike the already well-documented human serum proteome, there are still limitations regarding analyzing and interpreting the various physiological changes and disease states of the serum proteomes found in duck. Serum proteome in duck under the condition of mycotoxin contamination in feed has not yet been examined. This study aimed to introduce the characterization of the circulating proteomes in duck serum during exposure to aflatoxin B1 (AFB1).

Materials and Methods: Duck serum samples were collected from four experimental groups, gel-based mass spectrometry was then applied, and finally, 445 proteins were identified in pulled serum sample.

Results: Among these 445 proteins, 377 were present in at least one group from all. There were 35 proteins which were expressed when the duck was exposed to AFB1. The protein library that allows the identification of a large number of different proteins in duck serum will be enhanced by the addition of these peptide spectral data. It is noteworthy that chromodomain-helicase-DNA-binding protein 7 (CHD7) [Gallus gallus] was up-regulated in the group with the highest AFB1 contamination.

Conclusion: CHD7 protein might be somehow relative to aflatoxicosis in the duck that causes poor performance and economic loss. Moreover, other proteins present in duck serum were also added in the protein library.
Examining test anxiety in terms of academic expectations stress and motivation to study
Authors: Çelik Eyüp , Yıldırım Selami
Published: September 2019

The purpose of the study was to examine the relationship between the exam anxiety level of middle school students, their academic expectations stress, and level of motivation to study lesson. The study group consisted of 364 (184 female and 180 male) 7th and 8th grade secondary school students. Three instruments were used for data collection; Academic Expectations Stress Inventory, Motivation to Study for Adolescences Scale and Exam Anxiety Scale. Exam anxiety was chosen as dependent variable while academic expectations stress and motivation to study lesson were set as independent variables. In the current study, the relationship between variables was examined by Pearson correlation coefficient and whether independent variables predict dependent variable or not was tested by multiple regression analysis. As a result of correlational analysis, a positive and significant relationship was observed between teacher/parental expectations, self-expectations, amotivation and exam anxiety. On the other hand, intrinsic motivation and extrinsic motivation were not associated with exam anxiety. As a result of regression analysis, it was concluded that family/teacher expectations, self-expectations, amotivation, and extrinsic motivation significantly predicted exam anxiety.
HUBUNGAN KUALITAS PERNIKAHAN DENGAN KEBAHAGIAAN DAN KEPUASAN HIDUP PRIBADI: STUDI PADA INDIVIDU DENGAN USIA PERNIKAHAN 1-5 TAHUN DI BANDUNG
Authors: Kendhawati Lenny , Purba Fredrick Dermawan
Published: September 2019

Marriage is proven to be related to health and well-being of those involved in it. However, the status of marriage is not the most important, but how the quality of the marriage is the key to its positive effects. This study aims to examine a relationship between marital quality with happiness and life satisfaction. Data collection was conducted on 189 participants who have married with less than five years, said to be a critical period, which are living in the city of Bandung. The marital quality was measured using the Quality Marriage Index/QMI (6 items; α= .91), happiness was measured using the Happiness Thermometer at three time points (3 items; α= .83), and life satisfaction was measured using the Self-Anchoring Cantril Striving Scale at three time points (3 items; α= .47). The Spearman correlation test found that there was a significant positive relationship between the marital quality and happiness at three time points: today (rs= 0.431), last month (rs= 0.409), and throughout life (rs= 0.415) and life satisfaction at two time points: now (rs= 0.460) and next five years (rs= 0.281). In addition, life satisfaction of five years ago when subjects were still single was reported to be significantly lower compare to their life satisfaction this moment and predicted five years from now (p<0.001). These findings suggest that married couple giving more attention to improving the quality of their marriage relationship in order to be happier and satisfied with their life.
EVALUASI PROPERTI PSIKOMETRIS DAN PERBANDINGAN MODEL PENGUKURAN KONSTRUK SUBJECTIVE WELL-BEING
Authors: Akhtar Hanif
Published: September 2019

In the subjective well-being (SWB) measurement model, there are three models used by researchers, namely one-factor model (unidimension), two-factors model, and three-factor model. This study aims to test the validity and reliability of the SWB measure and compare the measurement model of SWB with confirmatory factor analysis method. The total subjects used in this study were 1,003 people with an age range of 14 - 50 years. Positive Affect Negative Affect Schedule (PANAS) and Satisfaction with Life Scale (SWLS) were used as SWB measurements. The results showed all dimensions have Alpha reliability above 0.80 with item-total correlation above 0.3. The results of exploratory factor analysis showed that all items are well distributed to their  dimensions with a factor loading above 0.4. Thus the scale in this study has satisfactory validity and reliability. The comparison of three hypothetical model showed that the three-factor model has the best model fit compared to the other two models. This finding provides evidence that the three components in the SWB construct are separate and independent of each other.
PENGUKURAN EMPLOYEE VOICE
Authors: Prihatsanti Unika , Fajriyanthi Fajriyanthi , Purwono Urip
Published: September 2019

Voice as a form of constructive expression in improving organizational functions and effectiveness has received great attention, especially in studies that explain the antecedents and consequences of employees’ voices. However, studies that explain psychometric properties of employee voice measurements are still limited. This article aimed to examine the psychometric properties, particularly construct validity, of employee voice measurement. In Study 1, three employee voice instruments were found through literature review from 23 articles in the last five years (2013-2018) from EBSCOhost and Proquest databases. In Study 2, based on the results of the literature review, the sound psychometric property test was carried out using confirmatory factor analysis. The results of data analysis prove that the Employee Voice scale of Liang, Farh, and Farh (2012) is an appropriate model for measuring employee voices with good validity and reliability.
MOTIVASI MENGAJAR GURU DITINJAU DARI KEPUASAN KEBUTUHAN BERDASAR DETERMINASI DIRI
Authors: Utomo Hanggara Budi , Suminar Dewi Retno , Hamidah Hamidah , Yulianto Dema
Published: September 2019

This study specifically aims to analyze the contribution of need satisfaction for autonomy, need satisfaction for competence, and need satisfaction for relatedness that is associated with teacher teaching motivation. The participants of this study were 84 early childhood education programs teachers in Kediri Regency and Tulungagung Regency (100% women, Mage = 31.71 years; SD = 8.86). Data were collected using the Basic Need Satisfaction in General Scale and the Autonomous Motivation for Teaching Questionnaire presented in Indonesian language. Data analysis in this study used descriptive statistical techniques and Pearson correlation technique. The results shows that there is no significant correlation between need satisfaction for autonomy with teaching motivation (r= .043; p = .585), there is significant correlation between need satisfaction for relatedness with teaching motivation (r= .208; p = .008), and there is significant correlation between need satisfaction for competence with teaching motivation (r= .167; p = .031). Understanding teaching motivation based on self-determination may help teachers in disadvantaged areas to improve and recognize self-potential, so that teachers can develop themselves by optimizing need satisfaction as a factor that influences the teacher's activities in teaching.
PERAN KESEIMBANGAN PEKERJAAN-KELUARGA DAN KUALITAS HIDUP TERHADAP KEBAHAGIAAN KERJA PADA PETUGAS PEMASYARAKATAN PEREMPUAN
Authors: Ratnaningsih Ika Zenita , Prasetyo Anggun Resdasari
Published: September 2019

Correctional officers encounter vulnerable working conditions that may cause psychological discomfort at work. This study aims to determine the role of work-family balance, and quality of life to happiness at work in female correctional officers. The subjects in this study were 87 female correctional officers from three correctional institutions in Semarang and Malang. The age of subjects ranged from 22 - 57 years (Mage = 41.80); and the work period of the subjects ranged from 3-34 years (Mtenure = 18.80). The sampling technique used was convenience sampling. Instruments that used for data collection were demographic data questionnaires, Work-Family Balance Scale (32 items; α = .898), WHOQOL-BREF Scale (26 items; α = .906), and Happiness at Work Scale (22 items; α = .801). Data analysis using multiple regression analysis show there a positive and significant correlation between work-family balance and happiness at work (r = .613; p <.001), there was a positive and significant correlation between quality of life and work happiness (r = .394; p <.001), furthermore work-family balance and quality of life together can predict happiness at work in female correctional officers (r = .633; R2 = .401; F = 28.115; p <.001). Work-family balance and quality of life effectively contributed 39.7%  to predicting happiness at work in female correctional officers.
Thoracic Manifestations of the Angiobehçet
Authors: Senhaji Lamiyae , Alami Badr , Amara Bouchra , El Biaze Mohammed , Benjelloun Mohamed Chakib , Serraj Mounia
Published: September 2019

Introduction: Vascular involvement in Behçet's disease affects 5 to 40% of patients. All vessels regardless of their type, size or location can be affected. This impairment is serious and can be life-threatening.Patients and methods: We collected 12 cases from January 2010 to January 2017. These were 11 men and 1 woman with an average age of 32.5 years. The venous involvement involved 11 patients with thrombosis of the upper vena cava (VCS) in 6 patients, thrombophlebitis of the lower limbs in 5 patients, and thrombosis of the lower vena cava and hepatic veins in 1 patient. The arterial involvement affected 9 patients (75%) with an aneurysm of the pulmonary arteries in 5 patients and a pulmonary embolism in 5 others. The cardiac involvement concerned half of the patients with pericarditis type in 3 patients and intra-cardiac thrombus in 4 patients.Conclusion: Thoracic involvement of the angiobehçet is frequent and severe, requiring rapid diagnosis and intense specific therapy to improve its prognosis.