bluuebunny/crossref_metadata_2025 huggingface dataset:
Mobile health technology, exercise adherence and optimal nutrition post rehabilitation among people with Parkinson’s Disease (mHEXANUT) – a randomized controlled trial protocol
Authors: Alnes Sigrid Ryeng , Lærum-Onsager Ellisiv , Bye Asta , Vistven Annette , Franzén Erika , Holst Mette , Brovold Therese
Published: March 2023

Background

Although it is well known that regular physical activity and exercise, as well as maintaining adequate nutritional status is important to delaying symptom development and maintaining physical capacity and function in people with Parkinson’s Disease (PD), many are unable to follow self-management recommendations. Active interventions have shown short-term effects, but there is a need for interventions that facilitate self-management over the course of the disease. Until now, no studies have combined exercise and nutritional interventions with an individual self-management approach in PD. Thus, we aim to examine the effect of a six-month mobile health technology(m-health)-based follow-up programme, focusing on self-management in exercise and nutrition, after an in-service interdisciplinary rehabilitation programme.

Methods

A single-blinded, two-group randomised controlled trial. Participants are Adults aged 40 or older, with idiopathic PD, Hoehn and Yahr 1–3, living at home. The intervention group receives a monthly, individualized, digital conversation with a PT, combined with use of an activity tracker. People at nutritional risk get additional digital-follow-up from a nutritional specialist. The control group receives usual care. The primary outcome is physical capacity, measured by 6-min walk test (6MWT). Secondary outcomes are nutritional status, Health related quality of life (HRQOL), physical function and exercise adherence. All measurements are performed at baseline, after 3 months and after 6 months. Sample size, based on primary outcome, is set at 100 participants randomized into the two arms, including an estimated 20% drop out.

Discussion

The increasing prevalence of PD globally makes it even more important to develop evidence-based interventions that can increase motivation to stay active, promote adequate nutritional status and improve self-management in people with PD. The individually tailored digital follow-up programme, based on evidence-based practice, has the potential to promote evidence-based decision-making and to empower people with PD to implement exercise and optimal nutrition in their daily lives and, hopefully, increase adherence to exercise and nutritional recommendations.

Trial registration

ClinicalTrials.gov (NCT04945876). First registration 01.03.2021.
Interventions to enhance medication adherence in pregnancy- a systematic review
Authors: Davies Anna , Mullin Sadie , Chapman Sarah , Barnard Katie , Bakhbakhi Danya , Ion Rachel , Neuberger Francesca , Standing Judith , Merriel Abi , Fraser Abigail , Burden Christy
Published: March 2023

Background

Sub-optimal medication adherence in pregnant women with chronic disease and pregnancy-related indications has the potential to adversely affect maternal and perinatal outcomes. Adherence to appropriate medications is advocated during and when planning pregnancy to reduce risk of adverse perinatal outcomes relating to chronic disease and pregnancy-related indications. We aimed to systematically identify effective interventions to promote medication adherence in women who are pregnant or planning to conceive and impact on perinatal, maternal disease-related and adherence outcomes.

Methods

Six bibliographic databases and two trial registries were searched from inception to 28th April 2022. We included quantitative studies evaluating medication adherence interventions in pregnant women and women planning pregnancy. Two reviewers selected studies and extracted data on study characteristics, outcomes, effectiveness, intervention description (TIDieR) and risk of bias (EPOC). Narrative synthesis was performed due to study population, intervention and outcome heterogeneity.

Results

Of 5614 citations, 13 were included. Five were RCTs, and eight non-randomised comparative studies. Participants had asthma (n = 2), HIV (n = 6), inflammatory bowel disease (IBD; n = 2), diabetes (n = 2) and risk of pre-eclampsia (n = 1). Interventions included education +/− counselling, financial incentives, text messaging, action plans, structured discussion and psychosocial support. One RCT found an effect  of the tested intervention on self-reported antiretroviral adherence but not objective adherence. Clinical outcomes were not evaluated. Seven non-randomised comparative studies found an association between the tested intervention and at least one outcome of interest: four found an association between receiving the intervention and both improved clinical or perinatal outcomes and adherence in women with IBD, gestational diabetes mellitus (GDM), and asthma. One study in women with IBD reported an association between receiving the intervention and maternal outcomes but not for self-reported adherence. Two studies measured only adherence outcomes and reported an association between receiving the intervention and self-reported and/or objective adherence in women with HIV and risk of pre-eclampsia. All studies had high or unclear risk of bias. Intervention reporting was adequate for replication in two studies according to the TIDieR checklist.

Conclusions

There is a need for high-quality RCTs reporting replicable interventions to evaluate medication adherence interventions in pregnant women and those planning pregnancy. These should assess both clinical and adherence outcomes.
Sleep quality and associated factors among adult people living with HIV on follow-up at Dessie Town Governmental Health Facilities Antiretroviral Therapy Clinics, Northeast, Ethiopia, 2020, a multicenter cross-sectional study
Authors: GebreEyesus Fisha Alebel , Degu Fatuma Seid , Yohanes Yeneabat Birhanu , Azagew Abere Woretaw
Published: March 2023

Background

Poor sleep quality is an important health problem in people living with HIV. The exact cause of sleep disturbance is not well known, but it may relate to HIV itself, antiretroviral drug side effects, and other HIV-related disorders. As a result, the purpose of this study was to assess sleep quality and associated factors among adult HIV patients on follow-up at Dessie Town governmental health facilities’ antiretroviral therapy clinics in Northeast Ethiopia in 2020.

Methods

A multi-center cross-sectional study was conducted among 419 adult people living with HIV/AIDS from February 1/2020 to April 22/2020 in Dessie Town governmental antiretroviral therapy clinics. A systematic random sampling method was used to select the study participants. An interviewer-administered method of data collection with a chart review was used. The Pittsburgh Sleep Quality Index was used to evaluate sleep disruption. A binary logistic regression was conducted to see the relationship between a dependent variable and independent variables. Variables with a p-value of < 0.05 and a 95% confidence interval were used to declare an association between factors and a dependent variable.

Results

A total of 419 study participants were enrolled in this study, with a response rate of 100%. The mean age of the study participants was 36 ± 6.5 SD years and 63.7% of the participants were female. The prevalence of poor sleep quality was found to be 36% (95% CI, 31–41%). Being female (AOR = 3.45, 95% CI: 1.52–7.79), viral loads 1000 copies/ml (AOR = 6.88, 95% CI: 2.79–16.9), CD4 cell count 200 cells/mm3 (AOR = 6.85, 95% CI: 2.42–19.39), WHO stage II and III (AOR = 4.29, 95% CI: 1.05–17.53), having anxiety (AOR = 10, 95% CI: 4.21–23.9.

Conclusion

The findings of this study showed that more than one-third of the study participants had poor-quality sleep at the Dessie Town Health Facility ART clinic. Being female, low CD4 cell counts, viral load ≥1000 copies/ml, WHO stage II and III, depression, anxiety, sleeping in a communal bedroom, and living alone were predictors of poor sleep quality.
The global dynamic transmissibility of COVID-19 and its influencing factors: an analysis of control measures from 176 countries
Authors: Wang Hongjian , Lan Yajia
Published: March 2023

Objective

To summarise the dynamic characteristics of COVID-19 transmissibility; To analyse and quantify the effect of control measures on controlling the transmissibility of COVID-19; To predict and compare the effectiveness of different control measures.

Methods

We used the basic reproduction number ($${R}_{0}$$

R
0

) to measure the transmissibility of COVID-19, the transmissibility of COVID-19 and control measures of 176 countries and regions from January 1, 2020 to May 14, 2022 were included in the study. The dynamic characteristics of COVID-19 transmissibility were summarised through descriptive research and a Dynamic Bayesian Network (DBN) model was constructed to quantify the effect of control measures on controlling the transmissibility of COVID-19.

Results

The results show that the spatial transmissibility of COVID-19 is high in Asia, Europe and Africa, the temporal transmissibility of COVID-19 increases with the epidemic of Beta and Omicron strains. Dynamic Bayesian Network (DBN) model shows that the transmissibility of COVID-19 is negatively correlated with control measures. Restricting population mobility has the strongest effect, nucleic acid testing (NAT) has a strong effect, and vaccination has the weakest effect.

Conclusion

Strict control measures are essential for controlling the COVID-19 outbreak; Restricting population mobility and nucleic acid testing (NAT) have significant impacts on controlling the COVID-19 transmissibility, while vaccination has no significant impact. In light of these findings, future control measures may include the widespread use of new NAT technology and the promotion of booster immunization.
Preparedness, resilience and unmet needs of informal caregivers of advanced cancer patients in a Regional Mission Hospital in Kenya: Qualitative Study
Authors: Too Wesley , Lelei Faith , Adam Mary , Halestrap Pete
Published: March 2023

Background

Cancer is the third highest cause of death in Kenya. Eighty percent of cancer cases arrive at advanced stages, when there is nothing that can be done to cure them, and palliative care is the best alternative. Although the majority of end-of-life care in Kenya is provided at home, little is known about the caregivers’ preparedness, resilience and continued unmet needs. The goal of this qualitative study was to explore caregivers’ perceived preparedness, resilience and continued unmet needs in their caregiving role to patients with advanced stages of cancer.

Methods

A purposive sampling method was used to identify and recruit twelve informal, home-based caregivers of patients with advanced cancer from Kijabe Palliative Clinic data base. Interviews were conducted in patients' homes. The data was analyzed using interpretive phenomenological analysis approach. Ethical considerations were observed. Participants were kept anonymous and confidentiality.

Results

Competing tasks, lack of preparedness in handling end-of-life care for patients in advanced stages of cancer were the main concerns. Continued unmet needs and financial stresses, and vulnerability for female caregivers all contributed to increased caregiver burden in this study. Caregivers were however determined and resilient amidst challenges that faced them, they exhibited hope against hopelessness. Some caregivers were vulnerable and faced potential for abuse following anticipated loss of their family member exacerbated psychosocial stress and needs

Conclusion

Informal caregivers had common unmet needs related to caring for their advanced cancer patients. Whilst family caregivers had huge caregiver burden, insurmountable practical challenges related to role overload and competing tasks, they remained resilient though unprepared in giving end of life care.

Recommendations

Caregivers should also be examined, prepared, and supported during clinic reviews. More research is needed on the use of telephones for caregiver follow-up, the impact of introducing caregiver-targeted screening tools on caregiver quality of life and their impact on enhancing caregiver well-being in order to prepare & support them adequately for the caregiving role.
Key transition technology of ski jumping based on inertial motion unit, kinematics and dynamics
Authors: Yu Jinglun , Ma Xinying , Qi Shuo , Liang Zhiqiang , Wei Zhen , Li Qi , Ni Weiguang , Wei Shutao , Zhang Shengnian
Published: March 2023

Background

The development and innovation of biomechanical measurement methods provide a solution to the problems in ski jumping research. At present, research on ski jumping mostly focuses on the local technical characteristics of different phases, but studies on the technology transition process are less.

Objectives

This study aims to evaluate a measurement system (i.e. the merging of 2D video recording, inertial measurement unit and wireless pressure insole) that can capture a wide range of sport performance and focus on the key transition technical characteristics.

Methods

The application validity of the Xsens motion capture system in ski jumping was verified under field conditions by comparing the lower limb joint angles of eight professional ski jumpers during the takeoff phase collected by different motion capture systems (Xsens and Simi high-speed camera). Subsequently, the key transition technical characteristics of eight ski jumpers were captured on the basis of the aforementioned measurement system.

Results

Validation results indicated that the joint angle point-by-point curve during the takeoff phase was highly correlated and had excellent agreement (0.966 ≤ r ≤ 0.998, P < 0.001). Joint root-mean-square error (RMSE) differences between model calculations were 5.967° for hip, 6.856° for knee and 4.009° for ankle.

Conclusions

Compared with 2D video recording, the Xsens system shows excellent agreement to ski jumping. Furthermore, the established measurement system can effectively capture the key transition technical characteristics of athletes, particularly in the dynamic changes of straight turn into arc in inrun, the adjustment of body posture and ski movement during early flight and landing preparation.
The precision of ROTEM EXTEM is decreased in hypocoagulable blood: a prospective observational study
Authors: Sunnersjö Lotta , Lindström Henrik , Schött Ulf , Törnquist Noa , Kander Thomas
Published: March 2023

Background

The use of viscoelastic tests is becoming increasingly popular. There is a paucity of validation of the reproducibility of varying coagulation states. Therefore, we aimed to study the coefficient of variation (CV) for the ROTEM EXTEM parameters clotting time (CT), clot formation time (CFT), alpha-angle and maximum clot firmness (MCF) in blood with varying degrees of coagulation strength. The hypothesis was that CV increases in states of hypocoagulability.

Methods

Critically ill patients and patients subjected to neurosurgery at a university hospital during three separate periods were included. Each blood sample was tested in eight parallel channels, yielding the CVs for the tested variables. In 25 patients, the blood samples were analysed both at baseline and after dilution with albumin 5%, as well as after being spiked with fibrinogen, simulating weak and strong coagulation.

Results

In total, 225 unique blood samples were collected from 91 patients. All samples were analysed in eight parallel ROTEM channels, resulting in 1,800 measurements. In hypocoagulable samples, defined as those with values outside the normal reference range, the CV of CT was higher (median (interquartile range)) (6.3% (5.1–9.5)) than for normocoagulable samples (5.1% (3.6–7.5)),p < 0.001. CFT showed no difference (p = 0.14), while the CV of alpha-angle was higher in hypocoagulable samples (3.6% (2.5–4.6)) than in normocoagulable samples (1.1% (0.8–1.6),p < 0.001. The CV of MCF was higher in hypocoagulable samples (1.8% (1.3–2.6)) than in normocoagulable samples (1.2% (0.9–1.7)),p < 0.001. The CV ranges for the different variables were as follows: CT: 1.2%–37%, CFT: 1.7%–30%, alpha-angle: 0.0%–17% and MCF: 0.0%–8.1%.

Conclusions

CVs for the EXTEM ROTEM parameters CT, alpha-angle, and MCF increased in hypocoagulable blood compared to blood with normal coagulation, confirming the hypothesis for CT, alpha-angle, and MCF but not for CFT. Furthermore, the CVs for CT and CFT were much higher than those for alpha-angle and MCF. The results demonstrate that EXTEM ROTEM results from patients with weak coagulation should be interpreted with the notion of limited precision and that procoagulative treatment, based only on ROTEM EXTEM, should be given with some caution.
SNHG15 enhances cisplatin resistance in lung adenocarcinoma by affecting the DNA repair capacity of cancer cells
Authors: Li Yong , Huang Hui-Qin , Huang Zheng-Hui , Yu Nan-Ding , Ye Xiang-Li , Jiang Mei-Chen , Chen Li-Min
Published: March 2023

Background

Lung adenocarcinoma (LUAD) is a prevalent malignancy. SNHG15 has been demonstrated to be oncogenic in many kinds of cancers, however the mechanism of SNHG15 in LUAD cisplatin (DDP) resistance remains unclear. In this study, we demonstrated the effect of SNHG15 on DDP resistance in LUAD and its related mechanism.

Methods

Bioinformatics analysis was adopted to assess SNHG15 expression in LUAD tissues and predict the downstream genes of SNHG15. The binding relationship between SNHG15 and downstream regulatory genes was proved through RNA immunoprecipitation, chromatin immunoprecipitation and dual-luciferase reporter assays. Cell counting kit-8 assay was adopted to evaluate LUAD cell viability, and gene expression was determined by Western blot and quantitative real-time polymerase chain reaction. We then performed comet assay to assess DNA damage. Cell apoptosis was detected by Tunnel assay. Xenograft animal models were created to test the function of SNHG15 in vivo.

Results

SNHG15 was up-regulated in LUAD cells. Moreover, SNHG15 was also highly expressed in drug-resistant LUAD cells. Down-regulated SNHG15 strengthened the sensitivity of LUAD cells to DDP and induced DNA damage. SNHG15 could elevate ECE2 expression through binding with E2F1, and it could induce DDP resistance by modulating the E2F1/ECE2 axis. In vivo experiments verified that the SNHG15 could enhance DDP resistance in LUAD tissue.

Conclusion

The results suggested that SNHG15 could up-regulate ECE2 expression by recruiting E2F1, thereby enhancing the DDP resistance of LUAD.
Sex differences in the anatomy of the anterior–superior acetabular rim in relation to pincer-type femoroacetabular impingement in Koreans: a three-dimensional quantitative analysis
Authors: Cho Byung Woo , Kwon Hyuck Min , Park Jun Young , Park Kwan Kyu , Kim Taehyun , Lee Woo-Suk
Published: March 2023

Purpose

The aim of this study was to compare the anatomical structures of the acetabular rim around the anterior inferior iliac spine (AIIS) ridge that indicate anterior focal coverage of acetabulum between the sexes using a three-dimensional (3D) model.

Methods

3D models of 71 adults (38 men and 33 women) with normal hip joints were used. Based on the location of the inflection point (IP) of the acetabular rim around the AIIS ridge, the patients were classified into anterior and posterior types, and the ratios thereof for each sex were compared. Coordinates for the IP, the most anterior point (MAP), and the most lateral point (MLP) were obtained and compared between the sexes and between anterior and posterior types.

Results

Coordinates for IPs in men were located anterior and inferior to those in women. MAP coordinates for men were located inferior to those for women, and MLP coordinates for men were located lateral and inferior to those for women. Comparing AIIS ridge types, we noted that coordinates for IPs of the anterior type were located medial, anterior, and inferior to those of the posterior type. Meanwhile, MAP coordinates of the anterior type were located inferior to those of the posterior type, and MLP coordinates of the anterior type were located lateral and inferior to those of the posterior type.

Conclusion

Anterior focal coverage of the acetabulum appears to differ between the sexes, and this difference may affect the development of pincer-type femoroacetabular impingement (FAI). Additionally, we found that anterior focal coverage differs according to anterior or posterior positioning of the bony prominence around the AIIS ridge, which may affect development of FAI.
Spondylolisthesis and mismatch deformity affect outcomes after total knee arthroplasty
Authors: Sheppard William L. , Chiou Daniel , Upfill-Brown Alexander , Shah Akash , Edogun Eghosa , Sassoon Adam , Park Don Y.
Published: March 2023

Background

Little published data currently exist regarding the potential relationships between spondylolisthesis, mismatch deformity, and clinical outcomes following total knee arthroplasty (TKA). We hypothesize that preexisting spondylolisthesis will result in decreased functional outcomes after TKA.

Methods

This retrospective cohort comparison of 933 TKAs was performed between January 2017 and 2020. TKAs were excluded if they were not performed for primary osteoarthritis (OA) or if preoperative lumbar radiographs were unavailable/inadequate to measure the degree of spondylolisthesis. Ninety-five TKAs were subsequently available for inclusion and divided into two groups: those with spondylolisthesis and those without. Within the spondylolisthesis cohort, pelvic incidence (PI) and lumbar lordosis (LL) were calculated on lateral radiographs to determine the difference (PI–LL). Radiographs with PI–LL > 10° were then categorized as having mismatch deformity (MD). The following clinical outcomes were compared between the groups: need for manipulation under anesthesia (MUA), total postoperative arc of motion (AOM) both pre-MUA or post-MUA/revision, incidence of flexion contracture, and a need for later revision.

Results

Forty-nine TKAs met the spondylolisthesis criteria, while 44 did not have spondylolisthesis. There were no significant differences in gender, body mass index, preoperative knee range of motion (ROM), preoperative AOM, or opiate use between the groups. TKAs with spondylolisthesis and concomitant MD were more likely to have MUA (p = 0.016), ROM < 0–120 (p < 0.014), and a decreased AOM (p < 0.02) without interventions.

Conclusion

Preexisting spondylolisthesis by itself may not have adverse effect clinical results following TKA. However, spondylolisthesis increases the likelihood of developing MD. In those with both spondylolisthesis and concomitant mismatch deformities, patients had statistically and clinically significantly decreased in postoperative ROM/AOM and increased need for MUA. Surgeons should consider clinical/radiographic assessments of patients with chronic back pain who present for total joint arthroplasty.

Level of evidence

Level 3.
Lower extremity amputations (LEAs) in a tertiary hospital in Togo: a retrospective analysis of clinical, biological, radiological, and therapeutic aspects
Authors: Kouevi-Koko T. E. , Amouzou K. S. , Sogan A. , Apeti S. , Dakey Y. E. L. , Abalo A.
Published: March 2023

Background

We analysed the clinical, biological, radiological profiles, and therapeutic patterns of the patients who underwent a surgical lower extremity amputation (LEA) in Togo from 2010 to 2020.

Methods

Retrospective analysis of clinical files of adult patients who underwent an LEA at a single centre (Sylvanus Olympio Teaching Hospital) from 1st January 2010 to 31st December 2020. Data were analysed by CDC Epi Info Version 7 and Microsoft Office Excel 2013 software.

Results

We included 245 cases. The mean age was 59.62 years (15.22 SD) (range: 15–90 years). The sex ratio was 1.99. The medical history of diabetes mellitus (DM) was found in 143/222 (64.41%) files. The amputation level found in 241/245 (98.37%) files was the leg in 133/241 (55.19%) patients, the knee in 14/241 (5.81%), the thigh in 83/241 (34.44%), and the foot in 11/241 (4.56%). The 143 patients with DM who underwent LEA had infectious and vascular diseases. Patients with previous LEAs were more likely to have the same limb affected than the contralateral one. The odds of trauma as an indication for LEA were twice as high in patients younger than 65 years compared to the older (OR = 2.095, 95% CI = 1.050–4.183). The mortality rate after LEA was 17/238 (7.14%). There was no significant difference between age, sex, presence or absence of DM, and early postoperative complications (P = 0.77; 0.96; 0.97). The mean duration of hospitalization marked in 241/245 (98.37%) files was 36.30 (1–278) days (36.20 SD). Patients with LEAs due to trauma had a significantly longer hospital admission than those with non-traumatic indications, F (3,237) = 5.505, P = 0.001.

Conclusions

Compared to previous decades, from 2010 to 2020, the average incidence of LEAs for all causes at Sylvanus Olympio Teaching Hospital (Lomé, Togo) decreased while the percentage of patients with DM who underwent LEAs increased. This setting imposes a multidisciplinary approach and information campaigns to prevent DM, cardiovascular diseases, and  relative complications.
The ELF3 transcription factor is associated with an epithelial phenotype and represses epithelial-mesenchymal transition
Authors: Subbalakshmi Ayalur Raghu , Sahoo Sarthak , Manjunatha Prakruthi , Goyal Shaurya , Kasiviswanathan Vignesh A , Mahesh Yeshwanth , Ramu Soundharya , McMullen Isabelle , Somarelli Jason A. , Jolly Mohit Kumar
Published: March 2023

Background

Epithelial-mesenchymal plasticity (EMP) involves bidirectional transitions between epithelial, mesenchymal and multiple intermediary hybrid epithelial/mesenchymal phenotypes. While the process of epithelial-mesenchymal transition (EMT) and its associated transcription factors are well-characterised, the transcription factors that promote mesenchymal-epithelial transition (MET) and stabilise hybrid E/M phenotypes are less well understood.

Results

Here, we analyse multiple publicly-available transcriptomic datasets at bulk and single-cell level and pinpoint ELF3 as a factor that is strongly associated with an epithelial phenotype and is inhibited during EMT. Using mechanism-based mathematical modelling, we also show that ELF3 inhibits the progression of EMT. This behaviour was also observed in the presence of an EMT inducing factor WT1. Our model predicts that the MET induction capacity of ELF3 is stronger than that of KLF4, but weaker than that of GRHL2. Finally, we show that ELF3 levels correlates with worse patient survival in a subset of solid tumour types.

Conclusion

ELF3 is shown to be inhibited during EMT progression and is also found to inhibit the progression of complete EMT suggesting that ELF3 may be able to counteract EMT induction, including in the presence of EMT-inducing factors, such as WT1. The analysis of patient survival data indicates that the prognostic capacity of ELF3 is specific to cell-of-origin or lineage.
Retraction Note: LNCAROD enhances hepatocellular carcinoma malignancy by activating glycolysis through induction of pyruvate kinase isoform PKM2
Authors: Jia Guizhi , Wang Yan , Lin Chengjie , Lai Shihui , Dai Hongliang , Wang Zhiqian , Dai Luo , Su Huizhao , Song Yanjie , Zhang Naiwen , Feng Yukuan , Tang Bo
Published: March 2023

This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1186/s13046-021-02090-7.
Inoculation with Azorhizobium caulinodans ORS571 enhances plant growth and salt tolerance of switchgrass (Panicum virgatum L.) seedlings
Authors: Chen Pengyang , Wei Qiannan , Yao Yifei , Wei Jiaqi , Qiu Li , Zhang Baohong , Liu Huawei
Published: March 2023

Background

Switchgrass (Panicum virgatum L.) is an important biofuel crop that may contribute to replacing petroleum fuels. However, slow seedling growth and soil salinization affect the growth and development of switchgrass. An increasing number of studies have shown that beneficial microorganisms promote plant growth and increase tolerance to salinity stress. However, the feasibility of inoculating switchgrass with Azorhizobium caulinodans ORS571 to enhance the growth and salt tolerance of its seedlings is unclear. Our previous study showed that A. caulinodans ORS571 could colonize wheat (Triticum aestivum L.) and thereby promote its growth and development and regulate the gene expression levels of microRNAs (miRNAs).

Results

In this study, we systematically studied the impact of A. caulinodans ORS571 on switchgrass growth and development and the response to salinity stress; we also studied the underlying mechanisms during these biological processes. Inoculation with A. caulinodans ORS571 significantly alleviated the effect of salt stress on seedling growth. Under normal conditions, A. caulinodans ORS571 significantly increased fresh plant weight, chlorophyll a content, protein content, and peroxidase (POD) activity in switchgrass seedlings. Under salt stress, the fresh weight, dry weight, shoot and root lengths, and chlorophyll contents were all significantly increased, and some of these parameters even recovered to normal levels after inoculation with A. caulinodans ORS571. Soluble sugar and protein contents and POD and superoxide dismutase (SOD) activities were also significantly increased, contrary to the results for proline. Additionally, A. caulinodans ORS571 may alleviate salt stress by regulating miRNAs. Twelve selected miRNAs were all upregulated to different degrees under salt stress in switchgrass seedlings. However, the levels of miR169, miR171, miR319, miR393, miR535, and miR854 were decreased significantly after inoculation with A. caulinodans ORS571 under salt stress, in contrast to the expression level of miR399.

Conclusion

This study revealed that A. caulinodans ORS571 increased the salt tolerance of switchgrass seedlings by increasing their water content, photosynthetic efficiency, osmotic pressure maintenance, and reactive oxygen species (ROS) scavenging abilities and regulating miRNA expression. This work provides a new, creative idea for improving the salt tolerance of switchgrass seedlings.
Methods in molecular biology and genetics: looking to the future
Authors: Forero Diego A. , Chand Vaibhav
Published: March 2023

In recent decades, advances in methods in molecular biology and genetics have revolutionized multiple areas of the life and health sciences. However, there remains a global need for the development of more refined and effective methods across these fields of research. In this current Collection, we aim to showcase articles presenting novel molecular biology and genetics techniques developed by scientists from around the world.
A mathematical characterization of anatomically consistent blood capillary networks
Authors: Bertolini Marina , Causin Paola , Turrini Cristina
Published: March 2023

Blood microcirculation is the site of control of tissue perfusion, blood-tissue exchange, and tissue blood volume. Despite the many irregularities, almost ubiquitously, one can recognize in microcirculation vessels a hierarchy of arterioles and venules, organized in tree-like structures, and capillary plexi, organized in net-like structures. Whilst for arterioles and venules it may be envisageable to obtain geometries needed for numerical simulations from imaging techniques, the size and numerosity of capillaries makes this task much more cumbersome. For this reason, it is interesting to study approaches to generate in silico-derived artifacts of capillary networks, even in view of machine-learning based approaches which require a large amount of samples for training. Artificial networks must correctly reflect proper metrics and topology, which in turn, will ensure with proper boundary conditions a physiological blood flux in the net and a sufficient nutrient distribution in the surrounding tissues. In this paper, we introduce the sequence of curves whose limit is the space filling Hilbert curve and we discuss its inherent properties and we obtain the backbone of the artificial capillary network from a suitable element of this sequence. The backbone represents a significant synthesis of basic metric features of the network and, in this context, its properties can be studied analytically. In this framework, the Hilbert curve is a malleable entity which allows to shape the backbone according to the physical indicators. In particular, two significant factors are shown to control the network topology and scaling: the iteration step of the Hilbert curve generation and the characteristic length of the REV, respectively. Based on the points we generate for a certain iteration step, we then obtain via spline interpolation a smoothed version of the curve, which fine–tunes the tortuosity. A volumetric construction is obtained building a tubular neighborhood of the backbone, whose metrics can be computed and tuned as well. Numerical simulations of the blood flow in the obtained geometry show the physical fields occurring in the artificial network.
An updated C. elegans nuclear body muscle transcriptome for studies in muscle formation and function
Authors: Schorr Anna L. , Mejia Alejandro Felix , Miranda Martina Y. , Mangone Marco
Published: March 2023

The body muscle is an important tissue used in organisms for proper viability and locomotion. Although this tissue is generally well studied and characterized, and many pathways have been elucidated throughout the years, we still lack a comprehensive understanding of its transcriptome and how it controls muscle development and function. Here, we have updated a nuclear FACS sorting-based methodology to isolate and sequence a high-quality muscle transcriptome from Caenorhabditis elegans mixed-stage animals. We have identified 2848 muscle-specific protein-coding genes, including 78 transcription factors and 206 protein-coding genes containing an RNA binding domain. We studied their interaction network, performed a detailed promoter analysis, and identified novel muscle-specific cis-acting elements. We have also identified 16 high-quality muscle-specific miRNAs, studied their function in vivo using fluorochrome-based analyses, and developed a high-quality C. elegans miRNA interactome incorporating other muscle-specific datasets produced by our lab and others.

Our study expands our understanding of how muscle tissue functions in C. elegans and

in turn provides results that can in the future be applied to humans to study muscular-related diseases.
Production of acetoin and its derivative tetramethylpyrazine from okara hydrolysate with Bacillus subtilis
Authors: Li Tao , Liu Ping , Guo Gege , Liu Zhaoxing , Zhong Lei , Guo Lianxia , Chen Cheng , Hao Ning , Ouyang Pingkai
Published: March 2023

Okara, a renewable biomass resource, is a promising fermentative raw material for the bio-production of value-added chemicals due to its abundance and low-costs. we developed a process for the enzymatic hydrolysis of okara, and then engineered Bacillus subtilis to utilize mixed sugars to produce acetoin in okara hydrolysis without the addition of a supplemental nitrogen source. Okara was initially hydrolyzed with cellulase, β-glucosidase, and pectinase to obtain okara hydrolysate containing mixed sugars (32.78 ± 0.23 g/L glucose, 1.43 ± 0.064 g/L arabinose, 7.74 ± 0.11 g/L galactose) and amino acids. In this study, Bacillus subtilis 168 was used as the acetoin-producing strain, and the key genes bdhA and acoA of the acetoin catabolism pathway were knocked out to improve the fermentation yield of acetoin. In order to utilize the galactose in the hydrolysate, the recombinant strain BS03 (Bacillus subtilis168∆bdhA∆acoA) was used to overexpress the arabinose transporter-encoding gene (araE) drive heterologous expression of the Leloir pathway gene (galKTE). The corn dry powder concentration was optimized to 29 g/L in the reducing sugar okara hydrolysate. The results show that the recombinant bacterium BS03 could still synthesize 11.79 g/L acetoin without using corn dry powder as a nitrogen source. Finally, using okara enzymatic hydrolysate as the carbon and nitrogen source, 11.11 g/L and 29.7 g/L acetoin were obtained by batch fermentation and fed-batch fermentation, respectively, which was further converted to 5.33 g/L and 13.37 g/L tetramethylpyrazine (TTMP) by reaction with an ammonium salt.
Biomechanical and clinical relationships between lower back pain and knee osteoarthritis: a systematic review
Authors: Amarasinghe Piyumi , Wadugodapitiya Surangika , Weerasekara Ishanka
Published: March 2023

Background

Osteoarthritis (OA) and lower back pain (LBP) are most common health problems which lead to pain and disability. This study aimed to systematically review the evidence to find any relationship between knee osteoarthritis (KOA) and LBP or any potential causation.

Methods

The databases of Scopus, MEDLINE, and Embase were searched from inception to 01 October 2022. Any study published in English assessing live humans over 18 years with KOA and LBP was eligible to be included. Studies were independently screened by two researchers. Data of the included studies were extracted based on the participants, outcomes related to knee and lumbar spine, reported association or causation between LBP and KOA, and study design. Data were narratively analyzed and presented as graphs and table. Methodology quality was assessed.

Results

Of 9953 titles and abstracts, duplicates were removed, and 7552 were screened. Altogether, 88 full texts were screened, and 13 were eligible for the final inclusion. There were some biomechanical and clinical causations were observed for the concurrent presence of LBP and KOA. Biomechanically, high pelvic incidence is a risk factor for development of spondylolisthesis and KOA. Clinically, knee pain intensity was higher in KOA when presents with LBP. Less than 20% of studies have justified their sample size during the quality assessment.

Discussion

Development and progression of KOA in patients with degenerative spondylolisthesis may be induced by significantly greater mismatches of lumbo-pelvic sagittal alignment. Elderly patients with degenerative lumbar spondylolisthesis and severe KOA reported a different pelvic morphology, increased sagittal malalignment with a lack of lumbar lordosis due to double-level listhesis, and greater knee flexion contracture than in patients with no to mild and moderate KOA. People with concurrent LBP and KOA have reported poor function with more disability. Both LBP and lumbar kyphosis indicate functional disability and knee symptoms in patients with KOA.

Conclusions

Different biomechanical and clinical causations were revealed for the concurrent existence of KOA and LBP. Therefore, careful assessment of both back and knee joints should be considered when treating KOA and vice versa.

Systematic review registration

PROSPERO CRD42022238571
Efficacy of disease management program used among patients with chronic heart failure: protocol for a systematic review and network meta-analysis
Authors: Duan Yongjie , Li Zongren , Zhong Qin , Rao Chongyou , Hua Yun , Wu Rilige , Dong Jing , Li Da , Wang Wenjun , He Kunlun
Published: March 2023

Background

A large number of studies have provided a variety of heart failure management program (HF-MP) intervention modes. It is generally believed that HF-MP is effective, but the question of which type of program works best, what level of support is needed for an intervention to be effective, and whether different subgroups of patients are best served by different types of programs is still confusing.

Methods

This study will search for published and unpublished randomized clinical trials in English examining HF-MP interventions in comparison with usual care. MEDLINE, Medlin In-Process and Non-Indexed, CENTRAL, CINAHL, EMBASE, and PsycINFO will be the databases. We will calibrate our eligibility criteria among the team. Each literature will be screened by at least two reviewers. Conflicts will be resolved through team discussion. A similar process will be used for data abstraction and quality appraisal. The results will be synthesized descriptively, and a network meta-analysis will be conducted if the studies are deemed methodologically, clinically, and statistically acceptable (e.g., I2 < 50%). Moreover, potential moderators of efficacy will be analyzed using a meta-regression.

Discussion

This study will reduce the clinical heterogeneity and statistical heterogeneity of review and meta-analysis through a more scientific classification method to determine the most effective HF-MP in different subgroups of heart failure patients with different human resource investments and different intervention methods, providing high-quality evidence and guidance for clinical practice.

Systematic review registration

PROSPERO CRD42021258521
Existence results to a Leray–Lions type problem on the Heisenberg Lie groups
Authors: Razani A. , Safari F.
Published: March 2023

Here, the existence and multiplicity of weak solutions to a generalized$(p(\cdot ),q(\cdot ))$(p(⋅),q(⋅))-Laplace equation involving Leray–Lions type operators with Hardy potential are studied under Dirichlet boundary conditions on the Heisenberg groups.
Memory sharing for handling memory overload on physical machines in cloud data centers
Authors: Ge Yaozhong , Tian Yu-Chu , Yu Zu-Guo , Zhang Weizhe
Published: March 2023

Over-committing computing resources is a widely adopted strategy for increased cluster utilization in Infrastructure as a Service (IaaS) cloud data centers. A potential consequence of over-committing computing resources is memory overload of physical machines (PMs). Memory overload occurs if memory usage exceeds a defined alarm threshold, exposing running computation tasks at a risk of being terminated by the operating system. A prevailing measure to handle memory overload of a PM is live migration of virtual machines (VMs). However, this not only consumes network bandwidth, CPU, and other resources, but also compels a temporary unavailability of the VMs being migrated. To handle memory overload, we present a memory sharing system in this paper for PMs in cloud data centers. With memory sharing, a PM automatically borrows memory from a remote PM when necessary, and releases the borrowed memory when memory overload disappears. This is implemented through swapping inactive memory pages to remote memory resource. Experimental studies conducted on InfiniBand-networked PMs show that the memory sharing system is fully functional. The measured throughput and latency are around 929 Mbps and 1.3 $$\mu$$
μ
s, respectively, on average for remote memory access. They are similar to those from accessing a local-volatile memory express solid-state drive, and thus are promising in real applications.
Examining global warming factors using self-organizing map and Granger causality network: a case from South Korea
Authors: Dhakal Thakur , Kim Tae-Su , Lee Do-Hun , Jang Gab-Sue
Published: March 2023

Background

Understanding and patterning the possible causal variables of global warming is attributed to the development of effective prevention and mitigation strategies for climate change. Therefore, we aimed to pattern and visualize the possible causal variables of global warming and measure the causality between them.

Methods

We patterned and visualized the time series (103 years, from 1918 to 2020) of global surface temperature (GTemp) data with the gross domestic product (GDP) per capita, human population (Pop), and carbon dioxide (CO2) emissions of South Korea using a self-organizing map (SOM) and examined the causable local feature of global warming using the Granger causality (GC) test. The time-series data were trained and mapped in 4 × 4 SOM grids, and causality networks between variables were examined using multivariate Granger test statistics.

Results

SOM patterned 103 years of data, and a dominant cluster contained continuous time-series data from 2007 to 2020. Similarly, the CO2emissions of South Korea were obtained as a predictable unidirectional causal variable for GTemp from GC analysis. Based on data from the past 34 years, significant causality (p-value = 0.01) was observed with the integrated effect of Pop, GDP, and CO2on GTemp.

Conclusion

This study patterned the time-series data using SOM and examined the causal relationship between variables using the GC test. The study framework could be used as a reference by future scholars, ecologists, and the United Nations Sustainable Development Goals.
Modeling effects of abiotic factors on the abundances of eight woody species in the Harana forest using artificial networks, random forest, and generalized linear models
Authors: Bedane Girma Ayele , Feyisa Gudina Legese , Wakjira Feyera Senbeta
Published: March 2023

Background

Abiotic factors exert different impacts on the abundance of individual tree species in the forest but little has been known about the impact of abiotic factors on the individual plant, particularly, in a tropical forest. This study identified the impact of abiotic factors on the abundances of Podocarpus falcatus, Croton macrostachyus, Celtis africana, Syzygium guineense, Olea capensis, Diospyros abyssinica, Feliucium decipenses, and Coffea arabica. A systematic sample design was used in the Harana forest, where 1122 plots were established to collect the abundance of species. Random forest (RF), artificial neural network (ANN), and generalized linear model (GLM) models were used to examine the impacts of topographic, climatic, and edaphic factors on the log abundances of woody species. The RF model was used to predict the spatial distribution maps of the log abundances of each species.

Results

The RF model achieved a better prediction accuracy with R2 = 71% and a mean squared error (MSE) of 0.28 for Feliucium decipenses. The RF model differentiated elevation, temperature, precipitation, clay, and potassium were the top variables that influenced the abundance of species. The ANN model showed that elevation induced a negative impact on the log abundances of all woody species. The GLM model reaffirmed the negative impact of elevation on all woody species except the log abundances of Syzygium guineense and Olea capensis. The ANN model indicated that soil organic matter (SOM) could positively affect the log abundances of all woody species. The GLM showed a similar positive impact of SOM, except for a negative impact on the log abundance of Celtis africana at p < 0.05. The spatial distributions of the log abundances of Coffee arabica, Filicium decipenses, and Celtis africana were confined to the eastern parts, while the log abundance of Olea capensis was limited to the western parts.

Conclusions

The impacts of abiotic factors on the abundance of woody species may vary with species. This ecological understanding could guide the restoration activity of individual species. The prediction maps in this study provide spatially explicit information which can enhance the successful implementation of species conservation.