bluuebunny/crossref_metadata_2025 huggingface dataset:
The strategy, function, and efficacy of the peritexts in Giles’ and Minford’s English translations of Liaozhai Zhiyi
Authors: Liang Zhao
Published: August 2024

Among all the English translations of Liaozhai Zhiyi, Giles’ and Minford’s translations received the most reviews from Western readers. Their translations are rich in peritexts, which make up a large proportion of the two translations. Based on Genette’s Paratexts theory and Batchelor’s latest research in this field, this article examines the two translations’ peritext strategy, main functions, and efficacy in the reception of Liaozhai Zhiyi with a view to seeing the roles that two types of peritexts play in the dissemination and reception of the book in English world. Looking closer to the two translations’ peritext strategies, including covers, acknowledgments, prefaces, introductions, notes, illustrations, and appendices, the author summarizes five main functions of the peritexts: to reveal to some extent the translator’s translating aims, to show the commissioner’s peritext strategies, to spread Chinese culture, and to consciously or unconsciously manifest socio-cultural context. In addition, the article, through contrastively analyzing readers’ reviews of two translations’ peritexts, concludes that the efficacy of the peritexts in Minford’s translation is much better than that in Giles’ translation, which means that the former may, to a large degree, help promote the reception of Liaozhai Zhiyi, while the latter may not. It is also anticipated that the research pattern can be used in the study of the roles peritexts and epitexts play in the reception of a wider genre of Chinese works so as to facilitate the going-global of Chinese works and culture.
Assessing the precision of machine learning for diagnosing pulmonary arterial hypertension: a systematic review and meta-analysis of diagnostic accuracy studies
Authors: Fadilah Akbar , Putri Valerinna Yogibuana Swastika , Puling Imke Maria Del Rosario , Willyanto Sebastian Emmanuel
Published: August 2024

Introduction

Pulmonary arterial hypertension (PAH) is a severe cardiovascular condition characterized by pulmonary vascular remodeling, increased resistance to blood flow, and eventual right heart failure. Right heart catheterization (RHC) is the gold standard diagnostic technique, but due to its invasiveness, it poses risks such as vessel and valve injury. In recent years, machine learning (ML) technologies have offered non-invasive alternatives combined with ML for improving the diagnosis of PAH.

Objectives

The study aimed to evaluate the diagnostic performance of various methods, such as electrocardiography (ECG), echocardiography, blood biomarkers, microRNA, chest x-ray, clinical codes, computed tomography (CT) scan, and magnetic resonance imaging (MRI), combined with ML in diagnosing PAH.

Methods

The outcomes of interest included sensitivity, specificity, area under the curve (AUC), positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR). This study employed the Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2) tool for quality appraisal and STATA V.12.0 for the meta-analysis.

Results

A comprehensive search across six databases resulted in 26 articles for examination. Twelve articles were categorized as low-risk, nine as moderate-risk, and five as high-risk. The overall diagnostic performance analysis demonstrated significant findings, with sensitivity at 81% (95% CI = 0.76–0.85, p < 0.001), specificity at 84% (95% CI = 0.77–0.88, p < 0.001), and an AUC of 89% (95% CI = 0.85–0.91). In the subgroup analysis, echocardiography displayed outstanding results, with a sensitivity value of 83% (95% CI = 0.72–0.91), specificity value of 93% (95% CI = 0.89–0.96), PLR value of 12.4 (95% CI = 6.8–22.9), and DOR value of 70 (95% CI = 23–231). ECG demonstrated excellent accuracy performance, with a sensitivity of 82% (95% CI = 0.80–0.84) and a specificity of 82% (95% CI = 0.78–0.84). Moreover, blood biomarkers exhibited the highest NLR value of 0.50 (95% CI = 0.42–0.59).

Conclusion

The implementation of echocardiography and ECG with ML for diagnosing PAH presents a promising alternative to RHC. This approach shows potential, as it achieves excellent diagnostic parameters, offering hope for more accessible and less invasive diagnostic methods.

Systematic Review Registration

PROSPERO (CRD42024496569).
Embodied curriculum mapping as a foundation for critical self-reflection and culture change
Authors: Basu Alo C.
Published: August 2024

This article describes a first-person qualitative research study to understand how common pedagogical approaches and cultural learning environments in STEM impact individuals. Prior to the study, the author observed that many students who were successful in advanced undergraduate neuroscience courses reported having struggled academically, socially, or emotionally in introductory STEM courses. The objective was to generate new ideas for approaches to address high rates of student attrition from introductory STEM courses related to this full range of issues through curriculum development. The author, a neurobiologist and tenured faculty member at the institution, audited four introductory STEM courses: Introduction to Cellular and Molecular Biology, Atoms & Molecules, Calculus 1, and Introductory Physics 2: Electromagnetism, Optics, and Modern Physics, offered by tenured colleagues in four different departments. A total of approximately 600 hours was spent by the author attending lectures, participating in classroom activities, completing homework, and studying for assessments. Homework, quizzes, and exams were marked by the course faculty using the same criteria as were applied for student work. In addition to measures of academic performance collected through the normal assessments, the author made note of her own emotional responses throughout the course of the study, which is why the process was dubbed ‘embodied’ curriculum mapping. The emotional responses revealed high levels of emotional stress associated with assessment, sensitivity to disciplinary boundary reinforcement, and a complex role of social and academic identity in all aspects of the experience. Given the first-person nature of the study, the potential future generalizability of the findings must be considered in light of the various revealed aspects of identity and experience of the author and subjected to further study using a broader range of empirical methodologies. The focus of this article’s conclusions and recommendations is therefore the impact of the process on the author and the potential for a similar process to serve as a foundation for critical self-reflection and learning for other STEM educators. The author recommends the process as a generative tool for pedagogical innovation and building faculty capacity for culture change in STEM.
The thymus road to a T cell: migration, selection, and atrophy
Authors: Ruiz Pérez Mario , Vandenabeele Peter , Tougaard Peter
Published: August 2024

The thymus plays a pivotal role in generating a highly-diverse repertoire of T lymphocytes while preventing autoimmunity. Thymus seeding progenitors (TSPs) are a heterogeneous group of multipotent progenitors that migrate to the thymus via CCR7 and CCR9 receptors. While NOTCH guides thymus progenitors toward T cell fate, the absence or disruption of NOTCH signaling renders the thymus microenvironment permissive to other cell fates. Following T cell commitment, developing T cells undergo multiple selection checkpoints by engaging with the extracellular matrix, and interacting with thymic epithelial cells (TECs) and other immune subsets across the different compartments of the thymus. The different selection checkpoints assess the T cell receptor (TCR) performance, with failure resulting in either repurposing (agonist selection), or cell death. Additionally, environmental cues such as inflammation and endocrine signaling induce acute thymus atrophy, contributing to the demise of most developing T cells during thymic selection. We discuss the occurrence of acute thymus atrophy in response to systemic inflammation. The thymus demonstrates high plasticity, shaping inflammation by abrogating T cell development and undergoing profound structural changes, and facilitating regeneration and restoration of T cell development once inflammation is resolved. Despite the challenges, thymic selection ensures a highly diverse T cell repertoire capable of discerning between self and non-self antigens, ultimately egressing to secondary lymphoid organs where they complete their maturation and exert their functions.
Exploring potential roles of long non-coding RNAs in cancer immunotherapy: a comprehensive review
Authors: Arshi Asghar , Mahmoudi Esmaeil , Raeisi Farzaneh , Dehghan Tezerjani Masoud , Bahramian Elham , Ahmed Yeasin , Peng Chun
Published: August 2024

Cancer treatment has long been fraught with challenges, including drug resistance, metastasis, and recurrence, making it one of the most difficult diseases to treat effectively. Traditional therapeutic approaches often fall short due to their inability to target cancer stem cells and the complex genetic and epigenetic landscape of tumors. In recent years, cancer immunotherapy has revolutionized the field, offering new hope and viable alternatives to conventional treatments. A particularly promising area of research focuses on non-coding RNAs (ncRNAs), especially long non-coding RNAs (lncRNAs), and their role in cancer resistance and the modulation of signaling pathways. To address these challenges, we performed a comprehensive review of recent studies on lncRNAs and their impact on cancer immunotherapy. Our review highlights the crucial roles that lncRNAs play in affecting both innate and adaptive immunity, thereby influencing the outcomes of cancer treatments. Key observations from our review indicate that lncRNAs can modify the tumor immune microenvironment, enhance immune cell infiltration, and regulate cytokine production, all of which contribute to tumor growth and resistance to therapies. These insights suggest that lncRNAs could serve as potential targets for precision medicine, opening up new avenues for developing more effective cancer immunotherapies. By compiling recent research on lncRNAs across various cancers, this review aims to shed light on their mechanisms within the tumor immune microenvironment.
Fever of unknown origin, blood and cerebrospinal fluid involvement: a leprosy case report
Authors: Chen Huan , Jiang Yumeng , Shi Ying , Zhang Wenyue , Jiang Haiqin , Wang Zhenzhen , Zeng Rui , Wang Hongsheng
Published: August 2024

Leprosy is a chronic infectious disease that mainly affects the skin and peripheral nerves, it can also invade deeper tissues and organs, including mucous membranes, lymph nodes, testes, eyes, and internal organs. Severe cases can result in deformities and disabilities. We encountered the case of a 39-year-old male with unexplained fever, headache and rash. The patient’s lesions were taken for histopathological examination and slit skin smear analysis. Further, the patient was detected of Mycobacterium leprae (M.leprae) nucleic acid sequences in the cerebrospinal fluid (CSF) and plasma, and M.leprae gene targets in the skin lesion tissue and blood. The patient was eventually diagnosed with multibacillary leprosy and type II leprosy reaction. These results suggest the possibility of bacteremia in patients with leprosy to some extent, and observation implies the potential invasion of CSF by M.leprae or its genetic material.
Simulating the near-field dynamic plume behavior of disposed fine sediments
Authors: Gundlach Jannek , Herbst Maximilian , Alex Antje Svenja , Zorndt Anna , Jordan Christian , Visscher Jan , Schlurmann Torsten
Published: August 2024

Projections of the effects of fine sediment disposals, relevant for managed estuaries and tidally influenced coastal areas, are typically based on numerical far-field models. For an accurate consideration of the disposal itself, near-field models are often needed. The open source near-field model, PROVER-M, simulates the relevant processes of the physics based, dynamic behavior of disposed fine sediments in coastal waters and is applied in this study. First, new small scale laboratory experiments of instantaneous disposals are presented, documenting the dynamic behavior of fine material disposed in shallow waters. Second, results of the PROVER-M model are shown for disposals in three different settings: (1) a field-scaled study complementary to the laboratory set-up, (2) a parametric study of sequentially varied model input and (3) a far-field model coupling for estimation of the PROVER-M impact. By comparing results of the laboratory experiments to the PROVER-M model, the physical behavior of PROVER-M is successfully validated. The impact of the ambient setting and dredged material parameters is evaluated by the PROVER-M simulations, where the results show non-linear, complex interdependencies of the input parameters on disposal properties in dependence of ambient site conditions and material composition. In this context, limits of the model application are assessed and critically discussed. Finally, an exemplary coupling to a far-field model based on a real set of disposals in the tidally influenced Weser estuary (Germany) illustrates the potential impact of PROVER-M for assessing far-field suspended sediment concentration (SSC), with increased maximum SSC values of up to 10%.
Exploring HLA-C methylation patterns and nutritional status in Kichwa mothers and infants from Tena, Ecuador
Authors: Velastegui Erick , Falconí Isaac B. , Garcia Valeria I. , Munizaga Gabriela , Matias de la Cruz Carmen , Segura Yaritza , Alcivar Kerly , Valencia Luz , Vera Edwin , Muñoz Mindy S. , Vanden Berghe Wim , Lebeer Sarah , Orellana-Manzano Andrea
Published: August 2024

Environment and lifestyle can affect the epigenome passed down from generation to generation. A mother’s nutrition can impact the methylation levels of her offspring’s epigenome, but it’s unclear which genes may be affected by malnutrition during gestation or early development. In this study, we examined the levels of methylated GC in the promoter region of HLA-C in mothers and infants from the Kichwa community in Ecuador. To do this, we analyzed saliva samples using bisulfite DNA sequencing. While we did not observe any significant differences in the mean methylation percentages in exon 1 of HLA-C between mothers and their infants after the first two years of lactation and life, respectively, we did find that infants tended to increase their methylation level during the first two years of life, while mothers tended to decrease it after the first two years of breastfeeding. When we compared methylation levels between mothers and infants using an ANOVA/posthoc Tukey test, we found that the average methylation for the entire population was less than 3% at T1 and T2. Although there was a tendency for infants to have higher methylation levels during their first two years of life and for mothers to have lower methylation levels after the first two years of breastfeeding, the mean values were not significantly different. However, we found a significant difference when we contrasted the data using a Kruskal-Wallis test at 0.05 for T1 AND T2 (p-value: 0.0148). Specifically, mothers had an average of X̅ = 2.06% and sons had X̅ = 1.57% at T2 (p-value: 0.7227), while the average for mothers was X̅ = 1.83% and for sons X̅ =1.77%. Finally, we identified three CpG motif nucleotide positions (32–33, 43–44, and 96–97) along the 122 bp analysis of HLA-C exon one, which was found to retain methylation patterns over time and is inherited from mother to offspring. Finally, our small pilot study did not reveal significant correlations between maternal and offspring nutritional status and DNA methylation levels of HLA-C exon one.
Granulomatosis with polyangiitis: clinical characteristics and updates in diagnosis
Authors: Potentas-Policewicz Malgorzata , Fijolek Justyna
Published: August 2024

Granulomatosis with polyangiitis (GPA) is a rare systemic disease characterized by granulomatous inflammation of the respiratory tract and necrotizing vasculitis of small and medium vessels often associated with the production of anti-neutrophil cytoplasmic antibodies (ANCA) directed mainly against leukocyte proteinase 3 (PR3). Usually, it involves upper airways, lungs, and kidneys, however any organ may be affected. The diagnosis is based on clinical, radiological, and serological findings. Biopsies, although strongly recommended, are not always feasible and often provides non-specific features. ANCA plays a crucial role in the diagnosis of GPA; nevertheless, ANCA detection is not a substitute for biopsy, which plays an important role in suspected cases, particularly when histological confirmation cannot be obtained. Significant advances have been made in classification criteria and phenotyping of the disease, particularly in determining the nuances between PR3-ANCA and myeloperoxidase (MPO)-ANCA vasculitis. This has led to better characterization of patients and the development of targeted treatment in the future. In addition, better identification of cytokine and immunological profiles may result in immuno-phenotyping becoming a new approach to identify patients with ANCA-associated vasculitis (AAV). Due to the chronic relapsing–remitting nature, strict follow-up of GPA is necessary to provide appropriate management. The search for the accurate marker of disease activity and to predict relapse is still ongoing and no predictor has been found to reliably guide therapeutic decision-making.
Application value of ultrasonic contrast imaging and ultrasonic parameters in post-transplant renal surgery
Authors: Liu Xinwei , Liu Dikuan , Long Meizhen , Chen Feng
Published: August 2024

Objective

Utilize VUEBOX quantitative analysis software to perform quantitative analysis dynamic ultrasound contrast images of post-transplant renal patients were assessed quantitatively five parameters of ultrasonic contrast and two-dimensional ultrasound are examined to explore their six value in Diagnosing Renal Graft Dysfunction.

Methods

A retrospective analysis was conducted on 73 post-transplant renal patients who underwent ultrasound contrast examinations at Yiyang Central Hospital from July 2022 to December 2023, They were diagnosed clinically and pathologically. Based on pathological and clinical diagnostic results, the patients were divided into three groups: 47 cases in the stable renal function group, 18 cases in the acute rejection (AR) group, and 8 cases in the delayed graft function (DGF) group. All patients underwent routine ultrasound and ultrasound contrast examinations post-transplantation. By comprehensively assessing renal function test results, clinical course, and pathological findings, differences in ultrasonic contrast quantitative parameters were analyzed. Additionally, ROC curves were constructed to evaluate the diagnostic efficacy of ultrasound contrast in discriminating between transplant renal rejection reactions and delayed renal function recovery.

Results

Statistically significant differences in characteristics, such as renal segmental artery resistance index, were observed among the stable renal function group, AR group, and DGF group (all P < 0.05), while peak systolic velocity showed no statistical significance (P > 0.05). Differences in cortical time to peak (TTP), medullary time to peak(TTP), main renal artery rise time (RT), main renal artery(TTP), and main renal artery fall time (FT) were statistically significant among the stable renal function group, AR group, and DGF group (P < 0.05). ROC curve analysis demonstrated that the accuracy of quantitative parameters for the DGF group and AR group was as follows: Renal artery TTP = Renal artery RT > Renal artery FT > Medulla TTP > Cortex TTP (with respective area under the curve values of 0.828, 0.828, 0.758, 0.742, 0.719). Among these, Renal artery TTP and Renal artery RT exhibited larger AUC values, with sensitivities of 87.5% each and specificities of 81.2 and 87.5%, respectively.

Conclusion

There are discernible differences in VUEBOX quantitative parameters between post-transplant AR and DGF cases, thereby providing imaging references for diagnosing of acute rejection and functional impairment following renal transplantation.
Revealing the lethal effects of Pasteurella multocida toxin on multiple organ systems
Authors: Yuan Jianlin , Li Jinfeng , Du Senyan , Wen Yiping , Wang Yiping , Lang Yi-Fei , Wu Rui , Yan Qi-Gui , Zhao Shan , Huang Xiaobo , Zhao Qin , Cao San-Jie
Published: August 2024

Pasteurella multocida toxin (PMT) is one of the most important virulence factors of Pasteurella multocida type D. Pasteurella multocida infection has caused enormous economic losses in the pig farming industry. Although it is well known that this bacterial infection causes progressive atrophic rhinitis, its effects on other organ tissues in pigs are unclear. In this study, PMT was expressed and purified, and the cytotoxic effects of PMT on four types of swine cells, LLC-PK1, PAM, IPEC, and ST, were investigated. LLC-PK1 exhibited the highest sensitivity to the cytotoxic effects of PMT. Our studies revealed that a PMT concentration of 0.1 μg/kg can lead to weight loss, whereas a PMT concentration of 0.5 μg/kg can lead to death in mice. PMT causes damage to the intestines, kidneys, lungs, livers, and spleens of mice. Furthermore, PMT caused acute death in pigs at treatment concentrations greater than 5 μg/kg; at PMT concentration of 2.5 μg/kg, weight loss occurred until death. PMT mainly caused damage to the hearts, lungs, livers, spleens and kidneys of pigs. The organ coefficient showed that damage to the heart and kidneys was the most severe and caused the renal pelvis and renal pyramid to dissolve and become cavitated. Pathology revealed hemorrhage in the lungs, liver, and spleen, and the kidneys were swollen and vacuolated, which was consistent with the damaged target organs in the mice. In conclusion, these findings demonstrate that PMT is extremely toxic in vitro and in vivo, causing damage to various organs of the body, especially the kidneys and lungs. This study provides a theoretical basis for the in-depth exploration of the cytotoxic effects of PMT on target organs.
Oppositions, joints, and targets: the attractors that are the glue of social interactions
Authors: Ham Jackson R. , Pellis Sergio M. , Pellis Vivien C.
Published: August 2024

Social interactions are often analyzed by scoring segments of predefined behavior and then statistically assessing numerical and sequential patterns to identify the structure of the encounters. However, this approach can miss the dynamics of the animals’ relationship over the course of the encounter, one that often involves invariant bonds, say a nose-to-nose orientation, with many different movements performed by both partners acting to counteract each other’s attempts to break or maintain the relationship. Moreover, these invariant bonds can switch from one configuration to another during an interaction, leading from one stable configuration to another. It is this stepwise sequence of configurational stabilities that lead to functional outcomes, such as mating, aggression, or predation. By focusing on the sequence of invariant relational configurations, the deep structure of interactions can be discerned. This deep structure can then be used to differentiate between compensatory movements, no matter how seemingly stereotyped they may appear, from movement patterns which are restricted to a particular form when more than one option is available. A dynamic perspective requires suitable tools for analysis, and such tools are highlighted as needed in describing particular interactions.
Potential impact of climate change on dietary grain protein content and its bioavailability-a mini review
Authors: Kashyap Sindhu , Reddy Bellam H. Rajashekar , Devi Sarita , Kurpad Anura V.
Published: August 2024

The changing global climate brings a gradual yet constant and adverse shift in crop production. Grain crop plants, particularly cereals and legumes, respond varyingly to adverse climate, including reduction in grain yield and changes to their nutrient densities. An understanding of specific changes to crop systems under differing climatic conditions can help in planning diets to meet human nutrient sufficiency. Grain protein content is also affected by adverse environmental factors. Deficits in protein yield, linked to changes in grain or seed protein and antinutrient concentrations, have been reported in major food crops when exposed to elevated carbon dioxide, high temperature, drought, and humidity. These changes, in addition to affecting the quantity of indispensable or essential amino acids (IAA), also impact their bioavailability. Therefore, it is important to assess consequences of climate change on grain protein quality. An important tool to measure grain protein quality, is measuring its digestibility at the level of the ileum and its IAA concentration, linked to a metric called the Digestible IAA Score (DIAAS). A minimally invasive technique called the dual isotope tracer technique, which measures IAA digestibility after simultaneous administration of two different intrinsically labelled protein sources, one a test protein (2H/15N) and one a reference protein (13C) of predetermined digestibility, has been used in evaluation of grain protein IAA digestibility, and promises more in the evaluation of changes based on climate. This review discusses climate induced changes to grain protein quality through the prism of IAA digestibility, using the dual isotope tracer technique.
Causes and predictors of unplanned reoperations within 30 days post laparoscopic pancreaticoduodenectomy: a comprehensive analysis
Authors: Zhang Shiwei , Yadav Dipesh Kumar , Wang Gaoqing , Jiang Yin , Zhang Jie , Yadav Rajesh Kumar , Singh Alina , Gao Guo , Chen Junyu , Mao Yefan , Wang Chengwei , Meng Yudi , Hua Yongfei
Published: August 2024

Objective

To delineate the risk factors and causes of unplanned reoperations within 30 days following laparoscopic pancreaticoduodenectomy (LPD).

Methods

A retrospective study reviewed 311 LPD patients at Ningbo Medical Center Li Huili Hospital from 2017 to 2024. Demographic and clinical parameters were analyzed using univariate and multivariate analyses, with P < 0.05 indicating statistical significance.

Results

Out of 311 patients, 23 (7.4%) required unplanned reoperations within 30 days post-LPD, primarily due to postoperative bleeding (82.6%). Other causes included anastomotic leakage, abdominal infection, and afferent loop obstruction. The reoperation intervals varied, with the majority occurring within 0 to 14 days post-surgery. Univariate analysis identified significant risk factors: diabetes, liver cirrhosis, elevated CRP on POD-3 and POD-7, pre-operative serum prealbumin < 0.15 g/L, prolonged operation time, intraoperative bleeding > 120 ml, vascular reconstruction, soft pancreatic texture, and a main pancreatic duct diameter ≤3 mm (all P < 0.05). Multivariate analysis confirmed independent risk factors: pre-operative serum prealbumin < 0.15 g/L (OR = 3.519, 95% CI 1.167-10.613), CRP on POD-7 (OR = 1.013, 95% CI 1.001-1.026), vascular reconstruction (OR = 9.897, 95% CI 2.405-40.733), soft pancreatic texture (OR = 5.243, 95% CI 1.628-16.885), and a main pancreatic duct diameter ≤3 mm (OR = 3.462, 95% CI 1.049-11.423), all associated with unplanned reoperation within 30 days post-LPD (all P < 0.05).

Conclusion

Postoperative bleeding is the primary cause of unplanned reoperations after LPD. Independent risk factors, confirmed by multivariate analysis, include low pre-operative serum prealbumin, elevated CRP on POD-7, vascular reconstruction, soft pancreatic texture, and a main pancreatic duct diameter of ≤3 mm. Comprehensive peri-operative management focusing on these risk factors can reduce the likelihood of unplanned reoperations and improve patient outcomes.
Pain pathophysiology and pharmacology of cattle: how improved understanding can enhance pain prevention, mitigation, and welfare
Authors: Zoltick Abigale H. , Mann Sabine , Coetzee Johann F.
Published: August 2024

Globally, humans rely on cattle for food production; however, there is rising societal concern surrounding the welfare of farm animals. From a young age, cattle raised for dairy and beef production experience pain caused by routine management procedures and common disease conditions. The fundamental mechanisms, nociceptive pathways, and central nervous system structures required for pain perception are highly conserved among mammalian species. However, there are limitations to a comparative approach to pain assessment due to interspecies differences in the expression of pain. The stoicism of prey species may impede pain identification and lead to the assumption that cattle lack pain sensitivity. This highlights the importance of establishing validated bovine-specific indicators of pain—a prerequisite for evidence-based pain assessment and mitigation. Our first objective is to provide an overview of pain pathophysiology to illustrate the importance of targeted analgesia in livestock medicine and the negative welfare outcomes associated with unmitigated pain. This is followed by a review of available analgesics, the regulations governing their use, and barriers to implementation of on-farm pain management. We then investigate the current research undertaken to evaluate the pain response in cattle—a critical aspect of the drug approval process. With an emphasis on emerging research in animal cognition and pain pathology, we conclude by discussing the significant influence that pain has on cattle welfare and areas where further research and modified practices are indicated.
Analysis of odontoid parameters in adolescent idiopathic scoliosis patients with different curve types
Authors: Li Kelin , Huang Longao , Xiao Qiongrun , Chen Weiyou , Du Yuwang , Jiang Hua
Published: August 2024

Introduction

Odontoid incidence (OI) is an important parameter that has recently been developed. However, there are currently no studies on OI in adolescent idiopathic scoliosis (AIS) patients. We aimed to examine the significance of OI in describing cervical sagittal alignment in AIS patients, explore the differences in cervical sagittal parameters among these patients with different curve types, and investigate the correlations between coronal deformity and cervical sagittal parameters in AIS patients.

Methods

The whole-spine anteroposterior and lateral plain radiographs of AIS patients were retrospectively analyzed. The parameters, including OI, odontoid tilt (OT), C2 slope, cervical lordosis (CL), T1 slope (T1S), and others, were measured. The AIS patients were grouped based on different curve types. Measurement parameters were compared between different groups. Pearson correlation analysis was performed for cervical sagittal parameters and Cobb angle.

Results

Ninety AIS patients were included, consisting of 14 males and 76 females. The main thoracic curve group exhibited a smaller OI compared to the main thoracolumbar/lumbar curve group (P < 0.05). In the AIS patients with a main thoracic curve, there was a significant correlation between Cobb angle and OI (r = −0.371, p < 0.01). The odontoid parameters exhibited significant correlations with several classic cervical sagittal parameters in AIS patients with different curve types. The validation of the formula CL = 0.36 × OI−0.67 × OT−0.69 × T1S showed a significant correlation (correlation coefficient = 0.917) between the actual measurements and the predicted values, with a determination coefficient of 0.842.

Conclusion

There may be a difference in OI between AIS patients with a main thoracic curve and those with a main thoracolumbar/lumbar curve. Odontoid parameters could be used to describe cervical sagittal alignment in AIS patients with different curve types.
The impact of detailed history taking: a case report of pediatric pulmonary and hepatic hydatid disease from Ecuador
Authors: Gallegos Camila , Vargas Ariel , Estrella David , Torres Alejandra , Andrade Carlos , Lascano Yazmina del Carmen , Garzon-Chavez Daniel , Aguilar Ana Cristina
Published: August 2024

Background

Echinococcosis is a uncommon condition in pediatric patients, and encompasses alveolar and cystic forms, predominantly affecting the lungs and liver. Transmission occurs primarily through zoonotic means, such as the contamination of water and food by infected dog and other canid feces. Diagnosis can be challenging due to nonspecific symptoms that often mimic pneumonia.

The case

A 6-year-old female patient from a rural area in Ecuador who initially presented with nonspecific symptoms indicative of pneumonia. However, further investigation into socio-environmental factors led to a diagnosis of pulmonary and hepatic hydatid disease.

Conclusion

The timely and accurate diagnosis of this infectious disease enabled the patient to receive appropriate treatment and surgical intervention, leading to her complete recovery.
Optimising infliximab induction dosing to achieve clinical remission in Chinese patients with Crohn’s disease
Authors: Zhu Kouzhu , Ding Xiaoliang , Xue Ling , Liu Linsheng , Wang Yan , Li Yun , Xi Qinhua , Pang Xueqin , Chen Weichang , Miao Liyan
Published: August 2024

Aims

A strategy based on therapeutic drug monitoring and population pharmacokinetic (popPK) models would likely increase the rate of clinical remission (CR) after infliximab (IFX) induction in patients with Crohn’s disease (CD). This study aimed to evaluate the relationship between early IFX levels and antibodies to infliximab (ATI) and CR at week 14 and simulate the probability of attaining the identified exposure target.

Methods

Patients with CD (n = 140) treated with IFX were enrolled to develop the popPK model. Of these, 43 moderate-to-severe patients with CD were followed up at week 14. Simulations were performed on patients with different dosage regimens and covariates.

Results

IFX levels >20.08 μg/mL at week 2, >18.44 μg/mL at week 6, and >3.08 μg/mL at week 14 were linked to CR. A one-compartment model fit the data best. The covariates influencing clearance were fat free mass, albumin and ATI levels. To achieve IFX levels >20.08 μg/mL at week 2, ≥400 mg IFX was predicted to be required in over 50% patients with 45–70 kg and 35–45 g/L albumin, except for patients with 70 kg and 30 g/L albumin.

Conclusion

IFX levels >20.08 μg/mL at week 2 and absence of ATI at week 14 are associated with CR. Optimising IFX induction dosing will be critical to achieve the target of early IFX levels associated with CR.
Dual roles of inflammatory programmed cell death in cancer: insights into pyroptosis and necroptosis
Authors: Wang Shuai , He Huanhuan , Qu Lailiang , Shen Qianhe , Dai Yihang
Published: August 2024

Programmed cell death (PCD) is essential for cellular homeostasis and defense against infections, with inflammatory forms like pyroptosis and necroptosis playing significant roles in cancer. Pyroptosis, mediated by caspases and gasdermin proteins, leads to cell lysis and inflammatory cytokine release. It has been implicated in various diseases, including cancer, where it can either suppress tumor growth or promote tumor progression through chronic inflammation. Necroptosis, involving RIPK1, RIPK3, and MLKL, serves as a backup mechanism when apoptosis is inhibited. In cancer, necroptosis can enhance immune responses or contribute to tumor progression. Both pathways have dual roles in cancer, acting as tumor suppressors or promoting a pro-tumorigenic environment depending on the context. This review explores the molecular mechanisms of pyroptosis and necroptosis, their roles in different cancers, and their potential as therapeutic targets. Understanding the context-dependent effects of these pathways is crucial for developing effective cancer therapies.
Respiratory pathology in the TDP-43 transgenic mouse model of amyotrophic lateral sclerosis
Authors: Biswas Debolina D. , Sethi Ronit , Woldeyohannes Yochebed , Scarrow Evelyn R. , El Haddad Léa , Lee Jane , ElMallah Mai K.
Published: August 2024

Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease that results in death within 2–5 years of diagnosis. Respiratory failure is the most common cause of death in ALS. Mutations in the transactive response DNA binding protein 43 (TDP-43) encoded by the TARDBP gene are associated with abnormal cellular aggregates in neurons of patients with both familial and sporadic ALS. The role of these abnormal aggregates on breathing is unclear. Since respiratory failure is a major cause of death in ALS, we sought to determine the role of TDP-43 mutations on the respiratory motor unit in the Prp-hTDP-43A315T mouse model – a model that expresses human TDP-43 containing the A315T mutation. We assessed breathing using whole-body plethysmography, and investigated neuropathology in hypoglossal and phrenic respiratory motor units. Postmortem studies included quantification of hypoglossal and putative phrenic motor neurons, activated microglia and astrocytes in respiratory control centers, and assessment of hypoglossal and phrenic nerves of TDP43A315T mice. The male TDP43A315T mice display an early onset of rapid progression of disease, and premature death (less than 15 weeks) compared to control mice and compared to female TDP43A315T mice who die between 20 and 35 weeks of age. The TDP43A315T mice have progressive and profound breathing deficits at baseline and during a respiratory challenge. Histologically, hypoglossal and putative phrenic motor neurons of TDP43A315T mice are decreased and have increased microglial and astrocyte activation, indicating pronounced neurodegeneration and neuroinflammation. Further, there is axonopathy and demyelination in the hypoglossal and phrenic nerve of TDP43A315T mice. Thus, the TDP-43A315T mice have significant respiratory pathology and neuropathology, which makes them a useful translatable model for the study of novel therapies on breathing in ALS.
Effects of Trichoderma harzianum and Bacillus subtilis on the root and soil microbiomes of the soybean plant INTACTA RR2 PRO™
Authors: Rigobelo Everlon Cid , de Andrade Luana Alves , Santos Carlos Henrique Barbosa , Frezarin Edvan Teciano , Sales Luziane Ramos , de Carvalho Lucas Amoroso Lopes , Guariz Pinheiro Daniel , Nicodemo Daniel , Babalola Olubukola Oluranti , Verdi Maria Caroline Quecine , Mondin Mateus , Desoignies Nicolas
Published: August 2024

Introduction

Soybean is a significant export product for several countries, including the United States and Brazil. There are numerous varieties of soybean. Among them, a genetically modified type known as INTACTA RR2 PRO™ has been designed to demonstrate resistance to glyphosate and to produce toxins that are lethal to several species of caterpillars. Limited information is available on the use of Trichoderma harzianum and Bacillus subtilis to promote plant growth and their impact on the plant microbiome.

Methods

This study aimed to evaluate the effects of these microorganisms on this soybean cultivar by analyzing parameters, such as root and shoot dry matter, nutritional status, and root and soil microbial diversity.

Results

The results indicated that treatments with B. subtilis alone or in combination with T. harzianum as seed or seed and soil applications significantly enhanced plant height and biomass compared to the other treatments and the control. No significant differences in phosphorus and nitrogen concentrations were detected across treatments, although some treatments showed close correlations with these nutrients. Microbial inoculations slightly influenced the soil and root microbiomes, with significant beta diversity differences between soil and root environments, but had a limited overall impact on community composition.

Discussion

The combined application of B. subtilis and T. harzianum particularly enhanced plant growth and promoted plant-associated microbial groups, such as Rhizobiaceae, optimizing plant-microbe interactions. Furthermore, the treatments resulted in a slight reduction in fungal richness and diversity.
Foliar spraying of boron prolongs preservation period of strawberry fruits by altering boron form and boron distribution in cell
Authors: Zhang Lei , Sun Changgang , Tian Hui , Xu Jiamin , Wu Xiuwen
Published: August 2024

Boron (B), an essential micronutrient for fruit development, also plays a crucial role in maintaining the shelf life of strawberries (Fragaria ananassa Duch.) by affecting cell wall structure and components. We investigated the distribution pattern of B within cells and cell walls in strawberry fruits under different B levels and revealed the relationship between the B distribution in cell walls and fruit firmness after harvesting. Foliar spraying of 0.1% H3BO3 promoted the growth of strawberry seedlings and improved fruit yield and flesh firmness by 45.7% and 25.6%. During the fruit softening and decay process, the content of bound B and cell wall-B decreased while more B was allocated to the protoplast and apoplast. The changes in B distribution in cells were attributed to cell damage during fruit decay, and B extended the freshness period of the fruits by alleviating the decrease of B distribution in cell walls. After leaving the fruits at room temperature for 10 h, the B content in different cell wall components significantly decreased, while foliar spraying of B alleviated the reduction of B content in covalently bound pectin (CBP), cellulose, and hemicellulose. Meanwhile, B spraying on fruit decreased the activity of cell wall degradation enzymes, including polygalacturonase (PG) and pectin lyase (PL), by 20.2% and 38.1%, while enhancing the demethylation of pectin by increasing pectin methylesterase (PME) activity from 21.6 U/g to 25.7 U/g. Thus, foliar spraying of 0.1% H3BO3 enhances the cross-linking of B with cell wall components and maintains cell wall structure, thereby prolonging the shelf life of strawberry fruits.
The role of psychological factors on improving work engagement among nurses
Authors: Abualruz Hasan , Rayan Ahmad , Al-Ghabeesh Suhair , Fawaz Mirna , Jaafeer Rayan , Qutami Batool , Alyami Hanan
Published: August 2024

Background

Work engagement is affected by many psychological variables including emotional intelligence, psychological empowerment, and resilience that are not well-studied among nurses.

Purpose

This study aims to examine the impact of emotional intelligence on the work engagement of critical care nurses, and the mediating role of resilience and psychological empowerment.

Methods

A descriptive cross-sectional design was adopted in this study among 150 critical care nurses at one university hospital in Saudi Arabia. Independent t-test and correlational analysis were used to assess relationships between study variables. A multi-step regression model was used to assess the mediatory effect.

Results

The results showed that a statistically significant positive association exists between each of the study variables (p < 0.01). The regression model showed that higher resilience (p < 0.001) and psychological empowerment (p < 0.001) predicted higher work engagement. The model predicted 33.3% of the changes in work engagement scores among critical care nurses.

Conclusion

To enhance work performance and quality of care rendered at critical care units, higher emphasis should be placed on emotional intelligence and other significant psychological variables.
Spatiotemporal heterogeneity of the association between socioeconomic development and health policy attention: a geographically and temporally weighted regression modeling study in China
Authors: He Rongxin , Wang Hongchuan , Liang Wannian
Published: August 2024

Objectives

Health policy attention (HPA) refers to the extent of attention given by governments to health issues in public policy and is generally influenced by socioeconomic development. This study aimed to examine the spatiotemporal heterogeneity and clustering of the associations between socioeconomic factors and HPA.

Study design

Longitudinal study.

Methods

This study examined the spatiotemporal heterogeneity of the association between public and provincial government attention, economic development, and demographic transition and HPA by using geographically and temporally weighted regression (GTWR). Word2Vec machine learning technology was utilized to calculate HPA data in 323 cities and independent variable data was collected in each city in China over the period of 2018–2021.

Results

The results showed that there is a substantial overall rise in HPA levels throughout China following the COVID-19 pandemic. Furthermore, the GTWR results revealed significant spatiotemporal heterogeneity in the associations between HPA and public and provincial government attention, economic development, and demographic transition, particularly in the context of COVID-19. The impact of provincial government attention on HPA decreased from the capital of the political center outward, while the impact of public financial investment decreased in less developed cities during the pandemic. It was only cities with high levels of aging are more likely to receive greater HPA.

Conclusion

The finding highlighted the remarkable spatial and temporal variations in the associations between the variables and HPA across different regions in China, emphasizing the need for region-specific policies to strengthen the focus on health by municipal governments.
Assessing educational disparities in COVID-19 related excess mortality in Spain: a population register-linked mortality study
Authors: Pulido José , Donat Marta , Moreno Almudena , Politi Julieta , Cea-Soriano Lucía , Sordo Luis , Mateo-Urdiales Alberto , Ronda Elena , Belza María José , Barrio Gregorio , Regidor Enrique
Published: August 2024

Introduction

Data on the increase in mortality during the COVID-19 pandemic based on individuals' socioeconomic positions are limited. This study examines this increase in mortality in Spain during the epidemic waves of 2020 and 2021.

Methods

We calculated the overall and cause-specific mortality rates during the 2017–2019 pre-pandemic period and four epidemic periods in 2020 and 2021 (first, second, third-fourth, and fifth-sixth waves). Mortality rates were analyzed based on educational levels (low, medium, and high) and across various age groups (25–64, 65–74, and 75+). The increase in mortality during each epidemic period compared to the pre-pandemic period was estimated using mortality rate ratios (MRR) derived from Poisson regression models.

Results

An inverse educational gradient in overall mortality was observed across all periods; however, this pattern was not consistent for COVID-19 mortality in some age groups. Among those aged 75 years and older, highly educated individuals showed higher COVID-19 mortality during the first wave. In the 25–64 age group, individuals with low education experienced the highest overall mortality increase, while those with high education had the lowest increase. The MRRs were 1.21 and 1.06 during the first wave and 1.12 and 0.97 during the last epidemic period. In the 65–74 age group, highly educated individuals showed the highest overall mortality increase during the first wave, whereas medium-educated individuals had the highest increase during the subsequent epidemic periods. Among those aged 75 and older, highly educated individuals exhibited the highest overall mortality increase while the individuals with low education showed the lowest overall mortality increment, except during the last epidemic period.

Conclusion

The varying educational patterns of COVID-19 mortality across different age groups contributed to the disparities of findings in increased overall mortality by education levels during the COVID-19 pandemic.