bluuebunny/crossref_metadata_2025 huggingface dataset:
Circulating mortalin in blood and activation of the alternative complement pathway as risk indicators in COVID-19 infection
Authors: Avraham Maya , Sinkovits György , Hurler Lisa , Prohászka Zoltán , Fishelson Zvi
Published: April 2024

Background

Mortalin/GRP75 is a ubiquitous mitochondrial chaperone related to the cytosolic heat shock protein 70. It protects cells from various types of damages and from senescence. Our goal was to determine whether COVID-19 patients have circulating mortalin in their blood and to assess its prognostic value in anticipating disease severity.

Methods

Mortalin was determined by ELISA in the sera of 83 COVID-19 patients enrolled in the study. Patients were categorized into 4 groups: critical patients who died (FATAL) or required intensive care and survived (ICU), patients of mild severity (hospitalized but not critical) who required nasal oxygen support (HOSP+O2), and patients who did not need oxygen therapy (HOSP).

Results

The mortalin concentration in the serum of all COVID-19 patients in the cohort was 194-2324 pg/mL. A comparison of the mortalin levels by peak severity among the various patient groups showed a highly significant difference between the HOSP and FATAL groups and a significant difference between the HOSP and the ICU groups. COVID-19 patients who eventually failed to survive had at hospitalization a markedly higher level of mortalin in their sera. Cox regression analysis revealed a high mortality hazard (HR=3.96, p<0.01) in patients with high mortalin circulating levels (above the median, ≥651 pg/mL). This was confirmed in survival curve analysis (Kaplan-Meier; p=0.0032, log-rank test). Mortalin remained an independent predictor of mortality even after adjusting for age and sex or various complement activation products. Complement activation data collected in an earlier study in the same cohort was compared regarding the mortalin levels. Patients with higher circulating mortalin levels also had higher levels of complement C3a but reduced levels of properdin.

Discussion

This is the first report on circulating mortalin in COVID-19 patients. Higher mortalin levels were associated with more severe illnesses and a higher risk of death. We claim that quantifying the blood levels of mortalin and activated complement proteins will provide important information on the prognosis of COVID-19 patients and will serve as a useful tool for guiding their clinical management and treatment.
The role of vitamin D in pediatric systemic lupus erythematosus - a double pawn in the immune and microbial balance
Authors: Lupu Vasile Valeriu , Lupu Ancuta , Jechel Elena , Starcea Iuliana Magdalena , Stoleriu Gabriela , Ioniuc Ileana , Azoicai Alice , Danielescu Ciprian , Knieling Anton , Borka-Balas Reka , Salaru Delia Lidia , Revenco Ninel , Fotea Silvia
Published: April 2024

Having increased popularity during the Covid-19 pandemic, vitamin D3 is currently impressing thanks to the numerous researches aimed at its interactions with the body’s homeostasis. At the same time, there is a peak in terms of recommendations for supplementation with it. Some of the studies focus on the link between autoimmune diseases and nutritional deficiencies, especially vitamin D3. Since the specialized literature aimed at children (patients between 0-18 years old) is far from equal to the informational diversity of the adult-centered branch, this review aims to bring up to date the relationship between the microbial and nutritional balance and the activity of pediatric systemic lupus erythematosus (pSLE). The desired practical purpose resides in a better understanding and an adequate, individualized management of the affected persons to reduce morbidity. The center of the summary is to establish the impact of hypovitaminosis D in the development and evolution of pediatric lupus erythematosus. We will address aspects related to the two entities of the impact played by vitamin D3 in the pathophysiological cascade of lupus, but also the risk of toxicity and its effects when the deficiency is over supplemented (hypervitaminosis D). We will debate the relationship of hypovitaminosis D with the modulation of immune function, the potentiation of inflammatory processes, the increase of oxidative stress, the perfusion of cognitive brain areas, the seasonal incidence of SLE and its severity. Finally, we review current knowledge, post-pandemic, regarding the hypovitaminosis D – pSLE relationship.
An empirical DNA-based identification of morphologically similar snappers (Lutjanus campechanus, Lutjanus purpureus) using a versatile bioinformatics workflow for the discovery and analysis of informative single-nucleotide polymorphisms
Authors: Literman Robert A. , Matos Mayara P. V. , Handy Sara M. , Deeds Jonathan R.
Published: April 2024

The commercially important species Lutjanus campechanus (Northern/Gulf red snapper) and Lutjanus purpureus (Southern/Caribbean red snapper) are the protagonists of a decade’s long taxonomic debate over their species delimitation, due in part to partial habitat overlap, extensive morphological similarity, and the lack of resolution when applying canonically reliable DNA barcoding approaches. In this study, we leveraged publicly available RAD-Seq data for L. campechanus and L. purpureus to identify species-informative single‐nucleotide polymorphisms (SNPs) at the genome scale that were successful in distinguishing the Northern and Southern red snappers, while also detecting individuals exhibiting introgression. This 4-step empirical approach demonstrates the value of applying novel bioinformatics pipelines to existing genome-scale data to maximize the distillation of informative subsets. Our results facilitate economically relevant species identification in addition to confirming or challenging species identifications for specimens with data in public databases. These findings and their applications will benefit future sustainability strategies and broader research questions surrounding these overfished and evolutionarily entangled snapper species.
Comparison between different advanced cannulation techniques for difficult biliary cannulation: a systematic review with a meta-analysis
Authors: Wang Liangjing , Wang Limin , Huang Nan , Li Tian , Shi Xiaohua , Zhu Qilin
Published: April 2024

Objective

This study aimed to systematically evaluate the efficacy and safety of the double-guidewire technique along with other methods (persistent standard cannulation techniques, transpancreatic sphincterotomy, and pancreatic stent-assisted technique) for difficult biliary cannulation.

Methods

Two researchers searched for literature on the efficacy and safety of the double-guidewire technique and other techniques in difficult biliary cannulation in databases, including PubMed, Embase, Cochrane, China National Knowledge Infrastructure, and Wanfang Data, based on the inclusion and exclusion criteria. The success rate of cannulation, duration of cannulation, post-ERCP pancreatitis, and overall postoperative complications were also analyzed using RevMan 5.4 software.

Results

In total, 20 randomized controlled trial (RCT) studies involving 2008 participants were identified. The success rate of cannulation in the double-guidewire technique was much higher than that in persistent standard cannulation techniques [RR = 1.37, 95%CI (1.05, 1.79), p = 0.02]. However, it was lower than the success rate observed with transpancreatic sphincterotomy [RR = 0.89, 95%CI (0.81, 0.97), p = 0.01]. There was no significance in post-ERCP pancreatitis [RR = 1.09, 95% CI (0.85, 1.40), p = 0.49], overall postoperative complications [RR = 0.90, 95% CI (0.56, 1.45), p = 0.66], and duration of cannulation [SMD = -0.14, 95%C I (−1.43, 1.15), p = 0.83] between the double-guidewire technique and other techniques.

Conclusion

This study demonstrated that the success rate of cannulation ranged from transpancreatic sphincterotomy to the double-guidewire technique and then to persistent standard cannulation techniques.
Effect of 0.01% atropine combined with orthokeratology lens on axial elongation: a 2-year randomized, double-masked, placebo-controlled, cross-over trial
Authors: Li Binbin , Yu Shiao , Gao Shasha , Sun Guangli , Pang Xuena , Li Xiuhong , Wang Ming , Zhang Fengyan , Fu Aicun
Published: April 2024

Purpose

To evaluate the effect of 0.01% atropine combined with orthokeratology (OK) lens on axial elongation in schoolchildren with myopia.

Methods

Sixty children aged 8–12 years with spherical equivalent refraction (SER) from -1.00D to -4.00D in both eyes were enrolled in this randomized, double-masked, placebo-controlled, cross-over trial. Children who had been wearing OK lenses for 2 months were randomly assigned into combination group (combination of OK lens and 0.01% atropine) for 1 year followed by control group (combination of OK lens and placebo) for another 1 year or vice versa. This trial was registered in the Chinese Clinical Trial Registry (Number: ChiCTR2000033904, 16/06/2020). The primary outcome was changes in axial length (AL). Data of right eyes were analyzed.

Results

There were statistically significant differences in the changes in AL between combination and control groups after generalized estimating equation model adjusting for age and baseline SER (p = 0.001). The mean axial elongation difference between combination and control groups was 0.10 mm in the first year (0.10 ± 0.13 mm vs. 0.20 ±0.15 mm; p = 0.01), and 0.09 mm in the second year (0.22 ± 0.10 mm vs. 0.13 ± 0.14 mm; p = 0.01), respectively. The mean axial elongation difference of two groups in the first year was similar to that in the second year during the cross-over treatment.

Conclusion

In central Mainland China in myopic children, the treatment of combination therapy is more effective than single OK lens in controlling axial elongation.
Identification of a linear B-cell epitope on the “puff” loop of the Senecavirus A VP2 protein involved in receptor binding
Authors: Zhou Hanrong , Sun Mingxia , Su Shibo , Meng Liang , Yang Wei , Yang Lan , Shi Xinqi , Li Xin , Wang Haiwei , Ma Hongwei , Cai Xuehui , Tang Yan-Dong , An Tongqing , Meng Fandan
Published: April 2024

Senecavirus A (SVA) is an important emerging swine pathogen that causes vesicular lesions in swine and acute death in newborn piglets. VP2 plays a significant role in the production of antibodies, which can be used in development of diagnostic tools and vaccines. Herein, the aim of the current study was to identify B-cell epitopes (BCEs) of SVA for generation of epitope-based SVA marker vaccine. Three monoclonal antibodies (mAbs), named 2E4, 1B8, and 2C7, against the SVA VP2 protein were obtained, and two novel linear BCEs, 177SLGTYYR183 and 266SPYFNGL272, were identified by peptide scanning. The epitope 177SLGTYYR183 was recognized by the mAb 1B8 and was fully exposed on the VP2 surface, and alanine scanning analysis revealed that it contained a high continuity of key amino acids. Importantly, we confirmed that 177SLGTYYR183 locates on “the puff” region within the VP2 EF loop, and contains three key amino acid residues involved in receptor binding. Moreover, a single mutation, Y182A, blocked the interaction of the mutant virus with the mAb 1B8, indicating that this mutation is the pivotal point for antibody recognition. In summary, the BCEs that identified in this study could be used to develop diagnostic tools and an epitope-based SVA marker vaccine.
Circulating plasma-derived extracellular vesicles expressing bone and kidney markers are associated with neurocognitive impairment in people living with HIV
Authors: Marques de Menezes Erika G. , Bowler Scott A. , Shikuma Cecilia M. , Ndhlovu Lishomwa C. , Norris Philip J.
Published: April 2024

Background

Although effective antiretroviral therapy (ART) has improved the life expectancy of people with HIV (PWH), the prevalence of milder forms of HIV-associated neurocognitive disorders (HAND) persist, and it is associated with systemic and neuro-inflammatory processes that could impact other organ systems. However, the complex signaling mechanisms between the bone-kidney systems and the brain in HAND remain unknown. Extracellular vesicles (EVs) play a potential role in inter-organ communication and are involved in regulating cell activity in distant tissues. In this study, we examined whether levels of EVs from bone-and kidney-related cells associate with cognitive dysfunction and explored the relationship between kidney-bone EV axis in PWH experiencing cognitive deficits.

Methods

EV subtypes were characterized in plasma from 61 PWH with either cognitive impairment (CI, n = 53) or normal cognition (NC, n = 8) based on the American Academy of Neurology criteria for HIV-associated dementia (HAD, n = 11), minor cognitive motor disorder (MCMD, n = 25) or asymptomatic neurocognitive impairment (ANI, n = 17) by spectral flow cytometry. EVs were profiled with markers reflecting bone and kidney cell origin. A support vector machine learning-based model was employed for analyses of EV phenotypes to predict the cognitive dysfunction.

Results

Plasma-EVs expressing osteocalcin, sclerostin, and nephrin were significantly higher in the cognitive impairment group compared to the normal cognition group. EVs bearing kidney cell markers correlated significantly with bone-derived EVs. A machine learning-based model, comprised of osteocalcin+, nephrin+, and CD24+ EVs predicted cognitive impairment in PWH on ART.

Conclusion

Our study reveals that neurocognitive impairment in PWH is associated with increased levels of plasma EVs enriched with the bone markers osteocalcin and sclerostin and the kidney marker nephrin, suggesting that these EV subtypes may be novel candidate biomarkers for disease-spanning neurocognitive dysfunction. Moreover, the relationship between bone-derived EVs with kidney-derived EVs may suggest their role in mediating inter-organ crosstalk in the pathogenesis of HIV-associated cognitive impairment.
Assessment of body composition in breast cancer patients: concordance between transverse computed tomography analysis at the fourth thoracic and third lumbar vertebrae
Authors: Daly Alex , Newman Lydia , Thomas Alexandra , Munro Alicia , Spence Cameron , Long Joe , Arnott Jonathan , Durkin Kesta , Layfield David , Heetun Adam , Wootton Stephen , Copson Ellen R. , Cutress Ramsey I.
Published: April 2024

Introduction

Specific body composition markers derived from L3 axial computed tomography (CT) images predict clinical cancer outcomes, including chemotherapy toxicity and survival. However, this method is only applicable to those undergoing lumbar (L3) CT scanning, which is not universally conducted in early breast cancer cases. This study aimed to evaluate CT analysis at T4 as a feasible alternative marker of body composition in breast cancer.

Method

All patients participated in the Investigating Outcomes from Breast Cancer: Correlating Genetic, Immunological, and Nutritional (BeGIN) Predictors observational cohort study (REC reference number: 14/EE/1297). Staging chest-abdomen-pelvic CT scan images from 24 women diagnosed with early breast cancer at University Hospital Southampton were analysed. Adipose tissue, skeletal muscle, and muscle attenuation were measured from the transverse CT slices’ cross-sectional area (CSA) at T4 and L3. Adipose tissue and skeletal muscle area measurements were adjusted for height. Spearman’s rank correlation coefficient analysis was used to determine concordance between body composition measurements using CT analysis at L3 and T4 regions.

Results

Derived estimates for total adipose tissue, subcutaneous adipose tissue, and intramuscular adipose tissue mass following adjustment for height were highly concordant when determined from CSAs of CT slices at T4 and L3 (Rs = 0.821, p < 0.001; Rs = 0.816, p < 0.001; and Rs = 0.830, p < 0.001). In this cohort, visceral adipose tissue (VAT) and skeletal muscle estimates following height adjustment were less concordant when measured by CT at T4 and L3 (Rs = 0.477, p = 0.039 and Rs = 0.578, p = 0.003). The assessment of muscle attenuation was also highly concordant when measured by CT at T4 and L3 (Rs = 0.840, p < 0.001).

Discussion

These results suggest that the CT analysis at T4 and L3 provides highly concordant markers for total adipose, subcutaneous adipose, and intramuscular adipose estimation, but not VAT, in this breast cancer population. High concordance between T4 and L3 was also found when assessing skeletal muscle attenuation. Lower concordance was observed for the estimates of skeletal muscle area, potentially explained by differences in the quantity and proportions of axial and appendicular muscle between the thorax and abdomen. Future studies will determine the value of T4 metrics as predictive tools for clinical outcomes in breast cancer.
Association between oxidative balance score in adults with and without chronic kidney disease: 2011–2028 NHANES
Authors: Cao Yuyu , Zhou Yishan , Zhong Yanghong , Liao Xianyong , Chen Xushan , Pi Ying
Published: April 2024

Introduction

Oxidative stress status is associated with CKD; however, few studies have investigated this association. The oxidative balance score (OBS) reflects systemic stress status and consists of 16 anti-and pro-oxidant dietary factors and four anti-and pro-oxidant lifestyle factors. Higher OBS implies exposure to more antioxidants. The purpose of this study was to explore the association between OBS and CKD.

Methods

We enrolled 8,134 study participants from the 2011–2018 National Health and Nutrition Examination Survey and obtained OBS by adding the 20 dietary and lifestyle factors. Based on OBS, the participants were divided into three groups. We performed logistic regression, subgroup analyzes, and restricted cubic spline regression to explore the association between OBS and CKD. In addition, we tested the adjusted model.

Results

OBS was negatively associated with CKD (OR: 0.54; 0.66, 0.82). After adjusting for all confounders, when dietary OBS was >20, the prevalence of CKD was reduced by 42% for each unit increase in OBS (p < 0.05). The negative associations of total OBS, dietary OBS, and lifestyle OBS with CKD were more significant in the female group. When the total OBS was ~20, the trend of decreasing prevalence in the female group was more significant.

Conclusion

OBS is negatively associated with chronic kidney disease.
Case report: Dual dabrafenib and trametinib therapy for treating BRAF V600E mutated lung adenocarcinoma with BRCA2 germline mutation post multiline progression
Authors: Zhang Huimin , Cong Xiaofeng , Yin Jiaxin , Chen Chen , Liu Ziling
Published: April 2024

The v-raf murine sarcoma viral oncogenic homolog B1 (BRAF) V600E is a rare mutation that functions as an oncogenic driver in patients with non-small cell lung cancer (NSCLC) leading to the overactivation of the RAS-RAF-MEK-ERK (MAPK) pathway and the subsequent uncontrolled cell proliferation. Understanding the mechanism behind BRAF mutation, its inhibition, and relationship to the upstream and downstream effector is essential for advancing treatment strategies for NSCLC patients with the BRAF V600E mutation. Next-generation sequencing studies have identified the presence of breast cancer susceptibility gene 1/2 (BRCA1/2) mutations in NSCLC patients, which are pathogenic variants associated with breast, ovarian, and prostate cancers. Although poly ADP-ribose polymerase (PARP) inhibitors are currently an approved treatment option for malignant tumors linked to BRCA1/2 pathogenic variants, the therapeutic potential of PARP inhibitors in NSCLC remains unclear. The development of genetic testing provides a platform for investigating the pathophysiological mechanisms of genetic mutations above. Here, we report a novel case of a middle-aged non-smoking female diagnosed with BRAF V600E and BRCA2 germline mutated lung adenocarcinoma, who had previously undergone a diverse array of cancer-targeted therapies, including PARP inhibitor, before the identification of the BRAF V600E mutation. Following this, a combination of dabrafenib and trametinib was administered and induced a rapid and positive response within two months. Our case not only highlights the importance of dynamic and repetitive genetic testing in managing patients, but contributes to the growing body of clinical evidence supporting the efficacy of BRAF/MEK co-inhibition in patients harboring a BRAF V600E mutation and provokes thinking for further research into the impact of PARP inhibitors in BRCA1/2-mutated NSCLC.
Chinese college students’ PGD symptoms and their relationship to cognitive variables: a latent profile analysis
Authors: Tian Weicui , Cui Yang , Liao Meiling , Huang Fajie
Published: April 2024

Bereavement is a commonly experienced grief event; however, bereavement can also trigger a number of psychological consequences, such as prolonged grief disorder (PGD). At present, the differences in prolonged grief disorder symptoms (PGD symptoms) among various individuals and how those symptoms relate to cognitive variables are unclear. In the present study, 817 Chinese college students with bereavement experience were selected as participants. Based on the evaluation results of their irrational beliefs, bereavement-related irrational beliefs, basic world assumptions, and PGD symptoms, an individual-centered latent profile analysis was used to divide a group with PGD symptoms into several subgroups and comprehensively examine the relationships between these subgroups and cognitive variables. (1) The severity of PGD symptoms among Chinese college students can be categorized into three subgroups: mild, moderate, and severe. (2) Cognitive variables such as irrational beliefs and basic world assumptions were all found to correlate with the severity of PGD symptoms; bereavement-related irrational beliefs was the variable with the largest correlation. However, for the first time, this study found that different dimensions of basic world assumptions had different directions of correlation, based on the severity of the PGD symptoms. Justice, control, randomness, and self-control had significantly positive correlations. Conversely, benevolence of the world, benevolence of people, and worthiness of the self had significantly negative correlations. These results have important reference value for cognitive behavioral therapy (CBT) treatment and interventions for PGD issues in Chinese college students.
Study of the influence of psychological mood on the performance and mental health of athletes in VR-aided basketball training
Authors: Wang Haiyan
Published: April 2024

The purpose of this study is to determine the influence of psychological mood on the performance and mental health of athletes during VR training. The study involved representatives of both men’s and women’s basketball teams from universities in China (62 girls and 65 boys, whose average age was 18.2). The participants were divided into 2 groups. Both groups trained regularly, except the experimental group used VR technology, while the control group did not. To study the physical performance of respondents, a complex psychophysiological test was used. The Warwick-Edinburgh Mental Well-Being Scale (WEMWBS) was used to assess the psychological mood and mental health of respondents. The VR training has been proven to increase the psychological attitude of basketball players during the training. Specifically, it has a positive effect on the psychophysiological performance indicators and mental health of athletes. Prospective research will be aimed at a comparative study of the impact of VR technology in the training process on the results of basketball players and representatives of other team sports.
Acoustic analysis of clients’ expression of self-compassion, self-criticism, and self-protection within emotion focused therapy video sessions
Authors: Bailey Ghazaleh , Halamová Júlia , Vráblová Viktória
Published: April 2024

Introduction

When it comes to the non-verbal communication of emotions, it is apparent that the human voice is one of the main ways of expressing emotion and is increasingly important in psychotherapeutic dialog. There is ample research focusing on the vocal expression of emotions. However, to date the analysis of the vocal quality of clients’ in-sessional emotional experience remains largely unexplored. Moreover, there is generally a gap within the psychotherapy literature in the understanding of the vocal character of self-compassion, self-criticism, and protective anger.

Methods

In this study we investigated how clients vocally convey self-compassion, self-protection and self-criticism in Emotion Focused therapy sessions. For this purpose we investigated 12 commercially available Emotion Focused Therapy videos that employed a two chair or empty chair dialog. Praat software was used for the acoustic analysis of the most common features – pitch (known as fundamental frequency or F0) and intensity (voice amplitude, i.e., loudness).

Results

Results showed that intensity was significantly higher for self-criticism and self-protection than for self-compassion. Regarding pitch the findings showed no significant differences between the three states.

Discussion

More research analyzing acoustic features in a larger number of cases is required to obtain a deeper understanding of clients’ vocal expression of self-compassion, self-protection and self-criticism in Emotion Focused Therapy.
Negative affect instability predicts elevated depressive and generalized anxiety disorder symptoms even when negative affect intensity is controlled for: an ecological momentary assessment study
Authors: Sultson Hedvig , Murd Carolina , Havik Merle , Konstabel Kenn
Published: April 2024

Introduction

Mood and anxiety disorders are characterized by abnormal levels of positive affect (PA), negative affect (NA) and changes in how emotions unfold over time. To better prevent and treat those disorders, it is crucial to determine which kind of indices of emotion dynamics best predict elevated depressive and generalized anxiety symptoms.

Methods

221 individuals (60 men; mean age = 46 years, SD = 15 years) completed a 7-day ecological momentary assessment study, where their positive and negative affective experience was assessed 5 times a day. For each participant, the intensity, instability, inertia, and differentiation of PA and NA were calculated. The Estonian Emotional State Questionnaire was used to assess depressive and generalized anxiety disorder (GAD) symptoms.

Results

We found that NA and PA intensity, and NA instability predicted elevated depressive and GAD symptoms. Models including NA instability alongside PA and NA intensity showed the best fit for both depression and generalized anxiety, as NA instability alongside other variables significantly increased the odds of having elevated depressive and GAD symptoms. Affective inertia, differentiation, and PA instability were not associated with depressive and GAD symptoms.

Discussion

In addition to the mean levels of affect, it is important to study other emotion dynamic indices such as NA instability, as these offer a more nuanced view of underlying emotion dysregulation processes. This could, in the long-term, help tailor more specific prevention and intervention methods for mood and anxiety disorders.
Antimicrobial activity of cell-free supernatant derived from Ligilactobacillus animalis SWLA-1 in a novel ex vivo canine corneal infection model
Authors: Jang Je-Hwan , Lee Hong-Jae , Kim Dong-Hwi , Lee Sang-Won , Kim Joon-Young
Published: April 2024

Introduction

Canine bacterial keratitis is a corneal infection that causes various symptoms, including visual impairment, and necessitates eye removal in severe cases. Staphylococcus pseudintermedius is a pathogen that causes significant bacterial keratitis in canine patients. Moreover, multi-drug resistant Staphylococcus pseudintermedius (MDRSP) has been reported in both humans and animals. Regarding treatment failure against multi-drug resistant (MDR) pathogens with classic antibiotics, antimicrobial compounds derived from probiotics have been suggested as an alternative approach.

Methods

Ligilactobacillus animalis SWLA-1 strain and its cell-free supernatant (CFS) have previously demonstrated potent antimicrobial activity against various MDR pathogenic bacteria. Based on this finding, we evaluated the anti-staphylococcal activity of CFS derived from Ligilactobacillus animalis SWLA-1 against MDRSP in a newly established ex vivo canine corneal infection model using fresh canine corneoscleral rims. Additionally, an in vitro cytotoxicity test using human keratocytes was performed.

Results and Discussion

CFS significantly inhibited the growth of MDRSP in the novel ex vivo model and did not exhibit any significant toxicity against keratocytes in vitro. Based on these results, the antimicrobial compounds in CFS show potential as a novel approach for MDR staphylococcal keratitis treatment.
Social Network Analysis of Farmers after the Private Cooperatives’ “Intervention” in a Rural Area of China—A Case Study of the XiangX Cooperative in Shandong Province
Authors: Sun Qingzhi , Yin Guanyi , Wei Wei , Zhang Zhan , Li Guanghao , Zhu Shenghao
Published: April 2024

In China, private-owned cooperatives are becoming increasingly involved in agricultural production. In order to find the key characteristics of smallholders’ social networks after the appearance of cooperatives and better organize different farmland operators, this study completed a field survey of 114 smallholders who adopted farmland trusteeship service of a private-owned cooperative in China and applied the social network analysis to reveal the following results. (1) Compared to the theoretical ideal value, smallholders’ social networks showed low network density, efficiency, and little relevancy. (2) In the social network of mechanical-sharing, neighbor, kinship, and labor-sharing relationships, some isolated nodes existed, but no isolated nodes are found in the synthetic network. (3) The mechanical-sharing relationship among smallholders was stronger than the other relationships. (4) Machinery owners, farmers whose plots are on the geometric center and experienced older farmers showed higher centralities in the network, but village cadres did not. (5) The centralities and QAP correlation coefficients among different networks inside the cooperative were lower than that inside a single village. As a result, this paper confirmed that the ability of cooperatives to organize farmers’ social networks is not ideal. Farmers’ trust of farmland to a cross-village cooperatives does not help them to form a larger social network than their villages. In the future, the answer to the question of “who will farm the land” will still lie with the professional farmers and highly autonomous cooperatives.
A Comprehensive Study of Generalized Lambert, Generalized Stieltjes, and Stieltjes–Poisson Transforms
Authors: Maan Jeetendrasingh , Negrín E. R.
Published: April 2024

In this paper, we explore the properties of the generalized Lambert transform, the L-transform, the generalized Stieltjes transform, and the Stieltjes–Poisson transform within the framework of Lebesgue spaces. We establish Parseval-type relations for each transform, providing a comprehensive analysis of their behaviour and mathematical characteristics.
Combined Observer-Based State Feedback and Optimized P/PI Control for a Robust Operation of Quadrotors
Authors: Benzinane Oussama , Rauh Andreas
Published: April 2024

This paper deals with a discrete-time observer-based state feedback control design by taking into consideration bounded parameter uncertainty, actuator faults, and stochastic noise in an inner control loop which is extended in a cascaded manner by outer PI- and P-control loops for velocity and position regulation. The aim of the corresponding subdivision of the quadrotor model is the treatment of the control design in a systematic manner. In the inner loop, linear matrix inequality techniques are employed for the placement of poles into a desired area within the complex z-plane. A robustification of the design towards noise is achieved by optimizing both control and observer gains simultaneously guaranteeing stability in a predefined bounded state domain. This procedure helps to reduce the sensitivity of the inner control loop towards changes induced by the outer one. Finally, a model-based optimization process is employed to tune the parameters of the outer P/PI controllers. To allow for the validation of accurate trajectory tracking, a comparison of the novel approach with the use of a standard extended Kalman filter-based linear-quadratic regulator synthesis is presented to demonstrate the superiority of the new design.
Resilience-Oriented Planning of Urban Distribution System Source–Network–Load–Storage in the Context of High-Penetrated Building-Integrated Resources
Authors: Zhu Sheng , Wang Ping , Lou Wei , Shen Shilin , Liu Tongtong , Yang Shu , Xiang Shizhe , Yang Xiaodong
Published: April 2024

Building-integrated flexible resources can offer economical availability to accommodate high-penetrated renewable energy sources (RESs), which can be potentially coordinated to achieve cost-effective supply. This paper proposes a resilience-oriented planning model of urban distribution system source–network–load–storage in the context of high-penetrated building-integrated resources. In this model, source–network–load–storage resources are cost-optimally planned, including the lines, soft open point (SOP), building-integrated photovoltaics (BIPVs), building-integrated wind turbine (BIWT), building-integrated energy storage system (ESS), etc. To enhance fault recovery capability during extreme faults, fault scenarios are incorporated into the distribution system operation via coupled multiple recovery stages. The resilience-oriented planning is a thorny problem due to its source–network–load–storage couplings, normal-fault couplings, etc. The original resilience-oriented planning is reformulated as a mixed-integer linear programming (MILP) problem, which can then be solved with a two-stage method and evaluated via a multi-dimensional evaluation metrics. The proposed planning methodology is benchmarked over a Portugal 54-node urban distribution system to verify the superiority and effectiveness on the system economy and resilience levels. Case studies show that the proposed methodology can exploit the optimal synergies of different source–network–load–storage components and enhance system dispatchability.
Numerical Investigation on the Seismic Behavior of Novel Precast Beam–Column Joints with Mechanical Connections
Authors: Zhuang Mei-Ling , Sun Chuanzhi , Yang Zhen , An Ran , Bai Liutao , Han Yixiang , Bao Guangdong
Published: April 2024

Traditional cast-in-place beam–column joints have the defects of high complexity and high construction difficulty, which seriously affect the efficiency and safety of the building construction line, and precast beam–column joints (PBCJs) can greatly improve the construction efficiency and quality. At present, the investigations on the seismic behavior of precast reinforced concrete structures are still mainly focused on experiments, while the numerical simulations for their own characteristics are still relatively lacking. In the present study, the seismic behavior of novel precast beam–column joints with mechanical connections (PBCJs-MCs) is investigated numerically. Based on the available experimental data, fiber models for four PBCJs-MCs are developed. Then, the simulated and experimental seismic behaviors of the prefabricated BCJs are compared and discussed. Finally, the factors influencing the seismic behavior of the PBCJs-MCs are further investigated numerically. The numerical results indicate that the fiber models can consider the effect of the bond–slip relationship of concrete and reinforcement under reciprocating loads. The relative errors of the simulated seismic behavior indexes are about 15%. The bearing capacity and displacement ductility coefficients of the PBCJs-MCs decrease rapidly as the shear-to-span ratio (λ) increases. It is recommended that the optimum λ for PBCJs-MCs is 2.0–2.5. The effect of the axial load ratio on the seismic behavior of PBCJs-MCs can be negligible in the case of the PBCJs-MCs with a moderate value of λ.
Changes in Outcomes of Macular Optical Coherence Tomography Angiography Following Surgery for Optic Disc Pit Maculopathy
Authors: Akıdan Melih , Erol Muhammet Kazım , Gedik Birumut , Doğan Mehmet Erkan , Başol Ibrahim , Süren Elçin
Published: April 2024

Purpose: we aimed to report on the optical coherence tomography angiography (OCTA) outcomes of eight patients with optic disc pit maculopathy (ODP-M) who were treated with 23-gauge pars plana vitrectomy (PPV). Methods: We examined sixteen eyes of eight patients—eight eyes with ODP-M and eight healthy fellow eyes. Fundus color photography, fundus autofluorescence, fundus fluorescein angiography, optical coherence tomography (OCT), and OCTA were performed. The vascular density, choriocapillaris blood flow (CCBF), and foveal avascular zone (FAZ) were analyzed using OCTA. Moreover, the correlation between the best-corrected visual acuity (BCVA) and macular OCTA parameters was assessed. Results: Compared with the healthy fellow eyes, the eyes with ODP-M preoperatively were found to have decreased BCVA, superficial capillary plexus (SCP) vascular density (i.e., total, foveal, parafoveal, and perifoveal), deep capillary plexus (DCP) vascular density (i.e., total, parafoveal, and perifoveal), and CCBF but a significantly increased FAZ (p < 0.05). When the eyes with ODP-M were analyzed pre- and postoperatively at month 12 after surgery, the BCVA, SCP vascular density (i.e., perifoveal), and CCBF had significantly increased, and the FAZ had significantly decreased (p < 0.05). When the eyes with ODP-M were compared with the healthy fellow eyes postoperatively at month 12, the BCVA, SCP, and DCP vascular density parameters had increased, along with CCBF, and the FAZ had decreased in eyes with ODP-M, though not to the levels of the healthy fellow eyes (p < 0.05). Moreover, a positive correlation was found between the postoperative BCVA and SCP total vascular density (p < 0.05). Conclusion: The BCVA and macular OCTA parameters improved in eyes with ODP-M at month 12 following surgery. However, the BCVA and OCTA of the eyes operated on did not reach the levels of the healthy fellow eyes, possibly due to impaired choroidal blood flow (CBF) recovery and the presence of a larger FAZ. In summary, OCTA seems to be useful for assessing qualitative and quantitative perioperative microvascular changes.
Factors Affecting Silica/Cellulose Nanocomposite Prepared via the Sol–Gel Technique: A Review
Authors: Shange Musawenkosi G. , Khumalo Nduduzo L. , Mohomane Samson M. , Motaung Tshwafo E.
Published: April 2024

Cellulose/silica nanocomposites, synthesised through the sol–gel technique, have garnered significant attention for their unique properties and diverse applications. The distinctive characteristics of these nanocomposites are influenced by a range of factors, including the cellulose-to-silica ratio, precursor concentration, pH, catalysts, solvent selection, temperature, processing techniques, and agitation. These variables play a pivotal role in determining the nanocomposites’ structure, morphology, and mechanical properties, facilitating tailoring for specific applications. Studies by Raabe et al. and Barud et al. demonstrated well-deposited silica nanoparticles within the interstitial spaces of cellulosic fibres, achieved through TEOS precursor hydrolysis and the subsequent condensation of hydroxyl groups on the cellulose fibre surface. The introduction of TEOS established a robust affinity between the inorganic filler and the polymer matrix, emphasising the substantial impact of TEOS concentration on the size and morphology of silica nanoparticles in the final composites. The successful functionalisation of cellulose fibres with the TEOS precursor via the sol–gel method was reported, resulting in reduced water uptake and enhanced mechanical strength due to the strong chemical interaction between silica and cellulose. In research conducted by Feng et al., the silica/cellulose composite exhibited reduced weight loss compared to the pristine cellulose matrix, with the integration of silica leading to an elevated temperature of composite degradation. Additionally, Ahmad et al. investigated the effects of silica addition to cellulose acetate (CA) and polyethylene glycol membranes, noting an increase in Young’s modulus, tensile strength, and elongation at break with silica incorporation. However, concentrations exceeding 4% (w/v) resulted in significant phase separations, leading to a decline in mechanical properties.
Unraveling the Interactions between Lithium and Twisted Graphene
Authors: Ramírez Maximo , De Luca Giorgio , Caputi Lorenzo
Published: April 2024

Graphene is undoubtedly the carbon allotrope that has attracted the attention of a myriad of researchers in the last decades more than any other. The interaction of external or intercalated Li and Li+ with graphene layers has been the subject of particular attention for its importance in the applications of graphene layers in Lithium Batteries (LiBs). It is well known that lithium atoms and Li+ can be found inside and/or outside the double layer of graphene, and the graphene layers are often twisted around its parallel plane to obtain twisted graphene with tuneable properties. Thus, in this research, the interactions between Li and Li+ with bilayer graphene and twisted bilayer graphene were investigated by a first-principles density functional theory method, considering the lithium atom and the cation at different symmetry positions and with two different adsorption configurations. Binding energies and equilibrium interlayer distances of filled graphene layers were obtained from the computed potential energy profiles. This work shows that the twisting can regulate the interaction of bilayer graphene with Li and Li+. The binding energies of Li+ systematically increase from bilayer graphene to twisted graphene regardless of twisted angles, while for lithium atoms, the binding energies decrease or remain substantially unchanged depending on the twist angles. This suggests a higher adsorption capacity of twisted graphene towards Li+, which is important for designing twisted graphene-based material for LiB anode coating. Furthermore, when the Li or Li+ is intercalated between two graphene layers, the equilibrium interlayer distances in the twisted layers increase compared to the unrotated bilayer, and the relaxation is more significant for Li+ with respect to Li. This suggests that the twisted graphene can better accommodate the cation in agreement with the above result. The outcomes of this research pave the way for the study of the selective properties of twisted graphene.
Energy-Level Interpretation of Carbazole Derivatives in Self-Assembling Monolayer
Authors: Grzibovskis Raitis , Aizstrauts Arturs , Pidluzhna Anna , Marcinskas Mantas , Magomedov Artiom , Karazhanov Smagul , Malinauskas Tadas , Getautis Vytautas , Vembris Aivars
Published: April 2024

Energy-level alignment is a crucial factor in the performance of thin-film devices, such as organic light-emitting diodes and photovoltaics. One way to adjust these energy levels is through chemical modification of the molecules involved. However, this approach may lead to unintended changes in the optical and/or electrical properties of the compound. An alternative method for energy-level adjustment at the interface is the use of self-assembling monolayers (SAMs). Initially, SAMs with passive spacers were employed, creating a surface dipole moment that altered the work function (WF) of the electrode. However, recent advancements have led to the synthesis of SAM molecules with active spacers. This development necessitates considering not only the modification of the electrode’s WF but also the ionization energy (IE) of the molecule itself. To measure both the IE of SAM molecules and their impact on the electrode’s WF, a relatively simple method is photo-electric emission spectroscopy. Solar cell performance parameters have a higher correlation coefficient with the ionization energy of SAM molecules with carbazole derivatives as spacers (up to 0.97) than the work function of the modified electrode (up to 0.88). Consequently, SAMs consisting of molecules with active spacers can be viewed as hole transport layers rather than interface layers.
Insights on Stability Constants and Structures of Complexes between Coumarin Derivatives and Pb(II) in Aqueous Media
Authors: Furia Emilia , Lettera Vincenzo , Napoli Anna , Aiello Donatella
Published: April 2024

In the frame of a systematic study on the sequestering ability of natural antioxidants towards metal cations, here the complexation of coumarin-3-carboxilic acid (HCCA) with Pb(II) and the overall stability constants of the resulting complexes, at 37 °C and in 0.16 M NaClO4, are discussed. Reaction of Pb(ClO4)2 with HCCA in an aqueous medium at a pH range from 2 to 6 and various ratios (1:1–1:10) yielded the Pb–CCA complexes, which were characterized spectrometrically by laser desorption ionization mass spectrometry (LD-MS). LD-MS has provided the composition and structure of Pb–CCA species according to the speciation model proposed on the basis of the potentiometric data. The graphic representation of the complex’s concentration curves is given by the distribution diagram, which provides a whole depiction of the species present in the solution at the selected pH ranges.
Quantum Size-Driven Spectral Variations in Pillar[n]arene Systems: A Density Functional Theory and Wave Function Assessment
Authors: Yao Cailian , Wang Tao
Published: April 2024

This study explores the quantum size effects on the optical properties of pillar[n]arene (n = 5, 6, 7, 8) utilizing density functional theory (DFT) and wave function analysis. The mechanisms of electron transitions in one-photon absorption (OPA) and two-photon absorption (TPA) spectra are investigated, alongside the calculation of electron circular dichroism (ECD) for these systems. Transition Density Matrix (TDM) and electron–hole pair density maps are employed to study the electron excitation characteristics, unveiling a notable size dependency. Analysis of the transition electric dipole moment (TEDM) and the transition magnetic dipole moment (TMDM) reveals the electromagnetic interaction mechanism within pillar[n]arene. Raman spectra computations further elucidate vibrational modes, while interactions with external environments are studied using electrostatic potential (ESP) analysis, and electron delocalization is assessed under an external magnetic field, providing insights into the magnetically induced current phenomena within these supramolecular structures. The thermal stability of pillar[n]arene was investigated by ab initio molecular dynamics (AIMD).