bluuebunny/crossref_metadata_2025 huggingface dataset:
Regulation, risk and health support work
Authors: Saks Mike , Allsop Judith
Published: February 2021

The limited regulation of support workers as compared to health professional workers and the ensuing risks are discussed in this chapter from a neo-Weberian standpoint. It is argued from the example of the United Kingdom, and especially England, that it may be in the public interest, in terms of protecting users of services and their carers from harm, to extend the existing regulatory framework in a number of modern societies. This is particularly so at a time when health professions themselves are coming under increasing challenge in the wake of adverse events and the subsequent publicity to which they have been exposed. The range of actions taken to mitigate risk include extending state regulatory controls, establishing registers for additional occupations, increasing employer and professional managerial controls in the public and private sector, and implementing additional requirements for continuing educational development. Health support workers, who are numerically the largest group of health personnel, remain under regulated and under researched. Regulatory confusion too often prevails in the complex division of labour in health care in relation to the risks of support workers to users – not to mention vulnerable support workers themselves in terms of their precarious work conditions, as discussed elsewhere in this volume.
The Impact of Direct Support Professional Turnover on the Health and Safety of People With Intellectual and Developmental Disabilities
Authors: Friedman Carli
Published: February 2021

Direct support professional (DSP) turnover significantly impacts both human service providers and people with intellectual and developmental disabilities (IDD). This study explored how DSP turnover impacts people with IDD's health and safety. We analyzed secondary data regarding DSP turnover and health and safety (i.e., emergency room visits, incidents of abuse and neglect, injuries, and behavioral events) from a random sample of 251 people with IDD. Findings revealed, regardless of their support needs, people who experienced DSP turnover had more emergency room visits, experienced more instances of abuse and neglect, and had more injuries than people who did not experience DSP turnover. Our findings suggest extended tenure of DSPs can help promote the health and safety of people with IDD.
Programmable disaggregated multi-dimensional S-BVT as an enabler for high capacity optical metro networks
Authors: Nadal Laia , Fàbrega Josep M. , Moreolo Michela Svaluto , Vílchez F. Javier , Casellas Ramon , Muñoz Raul , Vilalta Ricard , Martínez Ricardo
Published: February 2021

To meet the requirements of 5G high capacity optical metro networks, a programmable disaggregated multi-dimensional sliceable bandwidth/bitrate variable transceiver (S-BVT) is proposed. Specifically, transceiver multi-dimensionality is presented exploiting spatial, polarization, and spectral information as a solution to support network capacity and bandwidth scaling 5G requirements. Space division multiplexing is implemented by considering a 19-core multicore fiber enabling capacity scaling with the number of cores, whereas polarization division multiplexing is assessed enabling 50% spectral saving by considering two orthogonal polarization components. Finally, by the implementation of multi-band transmission systems, the optical bandwidth can be increased by a factor of 10, compared to a conventional C-band system. In these last two cases, the existing spectrum and network infrastructure can be reused, bringing new capabilities. In particular, in this work, multi-band transmission is assessed by exploiting the C-band and L-band. Additionally, disaggregation is also addressed at the transceiver level to enhance network flexibility, avoiding vendor lock-in while achieving efficiency and cost reduction. Disaggregation enables assembling open components, devices, and sub-systems into optical infrastructures and networks. On the other hand, the adoption of the software defined networking paradigm enables system/network programmability and reconfigurability, promoting an efficient use of the multi-dimensional network resources. Therefore, in this work, we analyze and experimentally demonstrate different S-BVT advanced functionalities suitable to support the stringent network requirements of 5G. These capabilities include rate/distance adaptability, programmability/configurability, disaggregation, and multi-dimensionality. Different network scenarios have been considered to assess the S-BVT functionalities, enabling Tb/s optical transmission.
In-vacuum measurements of optical scatter versus annealing temperature for amorphous Ta2O5 and TiO2:Ta2O5 thin films
Authors: Capote Elenna M. , Gleckl Amy , Guerrero Jazlyn , Rezac Michael , Wright Robert , Smith Joshua R.
Published: February 2021

Optical coatings formed from amorphous oxide thin films have many
applications in precision measurements. The Advanced Laser
Interferometer Gravitational-Wave Observatory (LIGO) and Advanced
Virgo use coatings of

S
i

O

2

(silica) and

T
i

O

2

:

T

a

2

O

5

(titania-doped tantala) and
post-deposition annealing to 500°C to achieve low thermal noise and
low optical absorption. Optical scattering by these coatings is a key
limit to the sensitivity of the detectors. This paper describes
optical scattering measurements for single-layer, ion-beam-sputtered
thin films on fused silica substrates: two samples of

T

a

2

O

5

and two of

T
i

O

2

:

T

a

2

O

5

. Using an imaging scatterometer at a
fixed scattering angle of 12.8°, in-situ changes in the optical
scatter of each sample were assessed during post-deposition annealing
to 500°C in vacuum. The scatter of three of the four coated optics was
observed to decrease during the annealing process, by 25–30% for
tantala and up to 74% for titania-doped tantala, while the scatter
from the fourth sample held constant. Angle-resolved scatter
measurements performed before and after vacuum annealing suggest some
improvement in three of the four samples. These results demonstrate
that post-deposition, high-temperature annealing of single-layer
tantala and titania-doped tantala thin films in vacuum does not lead
to an increase in scatter, and may actually improve their scatter.
Non-degenerate phase-sensitive amplification scheme using digital dispersion pre-equalization for unrepeated transmission
Authors: Shimizu Shimpei , Kazama Takushi , Kobayashi Takayuki , Umeki Takeshi , Enbutsu Koji , Kasahara Ryoichi , Miyamoto Yutaka
Published: February 2021

We experimentally demonstrate an ultra-low-noise pre-amplification using a non-degenerate phase-sensitive amplifier (ND-PSA) with an optically dispersion-unmanaged link. Chromatic dispersion (CD) compensation is required for phase-sensitive amplification after fiber transmission. In the conventional transmitter configuration for ND-PSAs in which phase-conjugated light (idler light) is optically generated, it is necessary to optically compensate for the CD, for example, by using dispersion-compensating fibers. In this work, we propose an ND-PSA scheme using a digitally generated idler and CD pre-equalization by means of digital signal processing. We conduct an unrepeated transmission over a 200-km single-mode fiber with a 10-Gbaud 64QAM signal using the periodically poled LiNbO3-based PSA. The experimental results demonstrate that the proposed ND-PSA scheme provides a low-noise pre-amplification that outperforms the EDFA without optical CD compensation.
Optical conveyor belt based on a plasmonic metasurface with polarization dependent hot spot arrays
Authors: Zhang Chi , Jiang Min , Chang Yao , Liu Yang , Wang Guanghui , Xu Fei , Lu Yanqing
Published: February 2021

In this Letter, we propose a novel metasurface conveyor belt with periodic orientated arrays of gold plasmonic elliptical elements (GPEEs), which can be continuously lit in a relay way by switching the polarization of the excitation beam and can be used to trap, transport, and sort particles. The array of the hot field can provide a larger trapping area and better stiffness. With the incident optical intensity of

0.08

m
W

/

µ

m

2

, the depth of the potential well could be as high as

10

K
B

T

. By setting a narrow interval between plasmonic ellipses in principal axes, it can help further enhance their directional resonant coupling and polarization dependence. Furthermore, based on consideration of the Brownian motion of trapped particles in aqueous solution, we analyzed its time response property of particle manipulation with different applied switching frequencies from a statistical point of view. As confirmed by numerical analysis, our design offers a novel scheme of particle sorting using a scalable hot spot array with better performance, which could be used in many on-chip optofluidic applications.
An integrated, modular approach to data science education in microbiology
Authors: Dill-McFarland Kimberly A. , König Stephan G. , Mazel Florent , Oliver David C. , McEwen Lisa M. , Hong Kris Y. , Hallam Steven J.
Published: February 2021

We live in an increasingly data-driven world, where high-throughput sequencing and mass spectrometry platforms are transforming biology into an information science. This has shifted major challenges in biological research from data generation and processing to interpretation and knowledge translation. However, postsecondary training in bioinformatics, or more generally data science for life scientists, lags behind current demand. In particular, development of accessible, undergraduate data science curricula has the potential to improve research and learning outcomes as well as better prepare students in the life sciences to thrive in public and private sector careers. Here, we describe the Experiential Data science for Undergraduate Cross-Disciplinary Education (EDUCE) initiative, which aims to progressively build data science competency across several years of integrated practice. Through EDUCE, students complete data science modules integrated into required and elective courses augmented with coordinated cocurricular activities. The EDUCE initiative draws on a community of practice consisting of teaching assistants (TAs), postdocs, instructors, and research faculty from multiple disciplines to overcome several reported barriers to data science for life scientists, including instructor capacity, student prior knowledge, and relevance to discipline-specific problems. Preliminary survey results indicate that even a single module improves student self-reported interest and/or experience in bioinformatics and computer science. Thus, EDUCE provides a flexible and extensible active learning framework for integration of data science curriculum into undergraduate courses and programs across the life sciences.
Evolutionary analyses of the major variant surface antigen-encoding genes reveal population structure of Plasmodium falciparum within and between continents
Authors: Tonkin-Hill Gerry , Ruybal-Pesántez Shazia , Tiedje Kathryn E. , Rougeron Virginie , Duffy Michael F. , Zakeri Sedigheh , Pumpaibool Tepanata , Harnyuttanakorn Pongchai , Branch OraLee H. , Ruiz-Mesía Lastenia , Rask Thomas S. , Prugnolle Franck , Papenfuss Anthony T. , Chan Yao-ban , Day Karen P.
Published: February 2021

Malaria remains a major public health problem in many countries. Unlike influenza and HIV, where diversity in immunodominant surface antigens is understood geographically to inform disease surveillance, relatively little is known about the global population structure of PfEMP1, the major variant surface antigen of the malaria parasitePlasmodium falciparum. The complexity of thevarmultigene family that encodes PfEMP1 and that diversifies by recombination, has so far precluded its use in malaria surveillance. Recent studies have demonstrated that cost-effective deep sequencing of the region ofvargenes encoding the PfEMP1 DBLα domain and subsequent classification of within host sequences at 96% identity to define unique DBLα types, can reveal structure and strain dynamics within countries. However, to date there has not been a comprehensive comparison of these DBLα types between countries. By leveraging a bioinformatic approach (jumping hidden Markov model) designed specifically for the analysis of recombination withinvargenes and applying it to a dataset of DBLα types from 10 countries, we are able to describe population structure of DBLα types at the global scale. The sensitivity of the approach allows for the comparison of the global dataset to ape samples ofPlasmodium Laveraniaspecies. Our analyses show that the evolution of the parasite population emerging out of Africa underlies current patterns of DBLα type diversity. Most importantly, we can distinguish geographic population structure within Africa between Gabon and Ghana in West Africa and Uganda in East Africa. Our evolutionary findings have translational implications in the context of globalization. Firstly, DBLα type diversity can provide a simple diagnostic framework for geographic surveillance of the rapidly evolving transmission dynamics ofP.falciparum. It can also inform efforts to understand the presence or absence of global, regional and local population immunity to major surface antigen variants. Additionally, we identify a number of highly conserved DBLα types that are present globally that may be of biological significance and warrant further characterization.
Vitamin D levels and risk of type 1 diabetes: A Mendelian randomization study
Authors: Manousaki Despoina , Harroud Adil , Mitchell Ruth E. , Ross Stephanie , Forgetta Vince , Timpson Nicholas J. , Smith George Davey , Polychronakos Constantin , Richards J Brent
Published: February 2021

Background

Vitamin D deficiency has been associated with type 1 diabetes in observational studies, but evidence from randomized controlled trials (RCTs) is lacking. The aim of this study was to test whether genetically decreased vitamin D levels are causally associated with type 1 diabetes using Mendelian randomization (MR).

Methods and findings

For our two-sample MR study, we selected as instruments single nucleotide polymorphisms (SNPs) that are strongly associated with 25-hydroxyvitamin D (25OHD) levels in a large vitamin D genome-wide association study (GWAS) on 443,734 Europeans and obtained their corresponding effect estimates on type 1 diabetes risk from a large meta-analysis of 12 type 1 diabetes GWAS studies (Ntot = 24,063, 9,358 cases, and 15,705 controls). In addition to the main analysis using inverse variance weighted MR, we applied 3 additional methods to control for pleiotropy (MR-Egger, weighted median, and mode-based estimate) and compared the respective MR estimates. We also undertook sensitivity analyses excluding SNPs with potential pleiotropic effects. We identified 69 lead independent common SNPs to be genome-wide significant for 25OHD, explaining 3.1% of the variance in 25OHD levels. MR analyses suggested that a 1 standard deviation (SD) decrease in standardized natural log-transformed 25OHD (corresponding to a 29-nmol/l change in 25OHD levels in vitamin D–insufficient individuals) was not associated with an increase in type 1 diabetes risk (inverse-variance weighted (IVW) MR odds ratio (OR) = 1.09, 95% CI: 0.86 to 1.40, p = 0.48). We obtained similar results using the 3 pleiotropy robust MR methods and in sensitivity analyses excluding SNPs associated with serum lipid levels, body composition, blood traits, and type 2 diabetes. Our findings indicate that decreased vitamin D levels did not have a substantial impact on risk of type 1 diabetes in the populations studied. Study limitations include an inability to exclude the existence of smaller associations and a lack of evidence from non-European populations.

Conclusions

Our findings suggest that 25OHD levels are unlikely to have a large effect on risk of type 1 diabetes, but larger MR studies or RCTs are needed to investigate small effects.
Number of people requiring post-exposure prophylaxis to end leprosy: A modeling study
Authors: Taal Anneke T. , Blok David J. , van Brakel Wim H. , de Vlas Sake J. , Richardus Jan Hendrik
Published: February 2021

Background

Worldwide, around 210,000 new cases of leprosy are detected annually. To end leprosy, i.e. zero new leprosy cases, preventive interventions such as contact tracing and post-exposure prophylaxis (PEP) are required. This study aims to estimate the number of people requiring PEP to reduce leprosy new case detection (NCD) at national and global level by 50% and 90%.

Methodology/Principal findings

The individual-based model SIMCOLEP was fitted to seven leprosy settings defined by NCD and MB proportion. Using data of all 110 countries with known leprosy patients in 2016, we assigned each country to one of these settings. We predicted the impact of administering PEP to about 25 contacts of leprosy patients on the annual NCD for 25 years and estimated the number of contacts requiring PEP per country for each year. The NCD trends show an increase in NCD in the first year (i.e. backlog cases) followed by a significant decrease thereafter. A reduction of 50% and 90% of new cases would be achieved in most countries in 5 and 22 years if 20.6 and 40.2 million people are treated with PEP over that period, respectively. For India, Brazil, and Indonesia together, a total of 32.9 million people requiring PEP to achieve a 90% reduction in 22 years.

Conclusion/Significance

The leprosy problem is far greater than the 210,000 new cases reported annually. Our model estimates of the number of people requiring PEP to achieve significant reduction of new leprosy cases can be used by policymakers and program managers to develop long-term strategies to end leprosy.
A performance comparison of supervised machine learning models for Covid-19 tweets sentiment analysis
Authors: Rustam Furqan , Khalid Madiha , Aslam Waqar , Rupapara Vaibhav , Mehmood Arif , Choi Gyu Sang
Published: February 2021

The spread of Covid-19 has resulted in worldwide health concerns. Social media is increasingly used to share news and opinions about it. A realistic assessment of the situation is necessary to utilize resources optimally and appropriately. In this research, we perform Covid-19 tweets sentiment analysis using a supervised machine learning approach. Identification of Covid-19 sentiments from tweets would allow informed decisions for better handling the current pandemic situation. The used dataset is extracted from Twitter using IDs as provided by the IEEE data port. Tweets are extracted by an in-house built crawler that uses the Tweepy library. The dataset is cleaned using the preprocessing techniques and sentiments are extracted using the TextBlob library. The contribution of this work is the performance evaluation of various machine learning classifiers using our proposed feature set. This set is formed by concatenating the bag-of-words and the term frequency-inverse document frequency. Tweets are classified as positive, neutral, or negative. Performance of classifiers is evaluated on the accuracy, precision, recall, andF1score. For completeness, further investigation is made on the dataset using the Long Short-Term Memory (LSTM) architecture of the deep learning model. The results show that Extra Trees Classifiers outperform all other models by achieving a 0.93 accuracy score using our proposed concatenated features set. The LSTM achieves low accuracy as compared to machine learning classifiers. To demonstrate the effectiveness of our proposed feature set, the results are compared with the Vader sentiment analysis technique based on the GloVe feature extraction approach.
Genome-wide identification and expression analysis of the NCED family in cotton (Gossypium hirsutum L.)
Authors: Li QingHua , Yu XianTao , Chen Long , Zhao Gang , Li ShiZhou , Zhou Hao , Dai Yu , Sun Na , Xie YongFei , Gao JunShan , Li DaHui , Sun Xu , Guo Ning
Published: February 2021

Abscisic acid (ABA) is an important plant hormone that plays multiple roles in regulating growth and development as well as in stress responses in plants. The NCED gene family includes key genes involved in the process of ABA synthesis. This gene family has been found in many species; however, the function of the NCED gene family in cotton is unclear. Here, a total of 23 NCED genes (designated as GhNCED1 to GhNCED23) were identified in cotton. Phylogenetic analysis indicated that the identified NCED proteins from cotton and Arabidopsis could be classified into 4 subgroups. Conserved motif analysis revealed that the gene structure and motif distribution of proteins within each subgroup were highly conserved. qRT-PCR and ABA content analyses indicated that NCED genes exhibited stage-specific expression patterns at tissue development stages. GhNCED5, GhNCED6 and GhNCED13 expression was similar to the change in ABA content, suggesting that this gene family plays a role in ABA synthesis. These results provide a better understanding of the potential functions of GhNCED genes.
Potential differences between the political attitudes of people with same-sex parents and people with different-sex parents: An exploratory assessment of first-year college students
Authors: Flores Andrew R. , Morrison Maisy
Published: February 2021

Children were often near the center of public debates about legal marriage recognition for same-sex couples.Obergefell v.Hodges(2015), the case that resulted in legal same-sex marriage recognition, stressed the importance of these children as one of many factors compelling the opinion. Estimates indicated same-sex couples were raising 200,000 children in the United States. Children raised by same-sex couples may be politically socialized in distinct ways compared to children of different-sex couples because lesbians, gay men, and bisexuals tend to hold distinct and progressive political viewpoints on a wide variety of issues. What are the political attitudes of people with same-sex parents? In this exploratory study, we analyze a large, representative survey of first-year college students across the United States; we find few differences between people with same-sex and different-sex parents, and some of those differences may be attributable to households and respondent characteristics. When on the rare occasion a difference exists, we find that people with same-sex female parents are more progressive, but people with same-sex male parents are more conservative. Gender differences also emerged, with some distinctive patterns between males with same-sex parents and females with same-sex parents.
From e-voucher to genomic data: Preserving archive specimens as demonstrated with medically important mosquitoes (Diptera: Culicidae) and kissing bugs (Hemiptera: Reduviidae)
Authors: Andrade Justi Silvia , Soghigian John , Pecor David B. , Caicedo-Quiroga Laura , Rutvisuttinunt Wiriya , Li Tao , Stevens Lori , Dorn Patricia L. , Wiegmann Brian , Linton Yvonne-Marie
Published: February 2021

Scientific collections such as the U.S. National Museum (USNM) are critical to filling knowledge gaps in molecular systematics studies. The global taxonomic impediment has resulted in a reduction of expert taxonomists generating new collections of rare or understudied taxa and these large historic collections may be the only reliable source of material for some taxa. Integrated systematics studies using both morphological examinations and DNA sequencing are often required for resolving many taxonomic issues but as DNA methods often require partial or complete destruction of a sample, there are many factors to consider before implementing destructive sampling of specimens within scientific collections. We present a methodology for the use of archive specimens that includes two crucial phases: 1) thoroughly documenting specimens destined for destructive sampling—a process called electronic vouchering, and 2) the pipeline used for whole genome sequencing of archived specimens, from extraction of genomic DNA to assembly of putative genomes with basic annotation. The process is presented for eleven specimens from two different insect subfamilies of medical importance to humans: Anophelinae (Diptera: Culicidae)—mosquitoes and Triatominae (Hemiptera: Reduviidae)—kissing bugs. Assembly of whole mitochondrial genome sequences of all 11 specimens along with the results of an ortholog search and BLAST against the NCBI nucleotide database are also presented.
Two distinct and separable processes underlie individual differences in algorithm adherence: Differences in predictions and differences in trust thresholds
Authors: Fenneman Achiel , Sickmann Joern , Pitz Thomas , Sanfey Alan G.
Published: February 2021

Algorithms play an increasingly ubiquitous and vitally important role in modern society. However, recent findings suggest substantial individual variability in the degree to which people make use of such algorithmic systems, with some users preferring the advice of algorithms whereas others selectively avoid algorithmic systems. The mechanisms that give rise to these individual differences are currently poorly understood. Previous studies have suggested two possible effects that may underlie this variability: users may differ in their predictions of the efficacy of algorithmic systems, and/or in the relative thresholds they hold to place trust in these systems. Based on a novel judgment task with a large number of within-subject repetitions, here we report evidence that both mechanisms exert an effect on experimental participant’s degree of algorithm adherence, but, importantly, that these two mechanisms are independent from each-other. Furthermore, participants are more likely to place their trust in an algorithmically managed fund if their first exposure to the task was with an algorithmic manager. These findings open the door for future research into the mechanisms driving individual differences in algorithm adherence, and allow for novel interventions to increase adherence to algorithms.
Modified ocular surface disease index as a screening criteria for dry eye syndrome presenting after successful dacryocystorhinostomy
Authors: Kang Tae Seen , Cho Jin , Kim Jaeyoung , Sung Jae Yun , Kim Ju Mi , Kim Kyoung Nam , Lee Sung Bok
Published: February 2021

Objective

To find preoperative screening criteria for dry eye syndrome (DES) that present after successful endoscopic dacryocystorhinostomy (EDCR).

Methods

We retrospectively analyzed medical records of 110 patients who underwent EDCR for nasolacrimal duct obstruction. DES diagnostic criteria were defined as tear break-up time (TBUT) less than 10 seconds, and ocular surface disease index (OSDI) score greater than 13 points. After EDCR, patients were divided into DES group and control group according to the DES diagnostic criteria. Preoperative OSDI score alone or in combination of preoperative TBUT and OSDI score were used to find screening criteria, which could discriminate the two groups preoperatively with a high positive predictive value (PPV). Criteria A was set same as the diagnostic criteria of DES, and Criteria B and C were set to improve PPV by increasing specificity while maintaining similar sensitivity to Criteria A.

Results

Thirty patients (27.3%) were diagnosed with DES after EDCR, while 80 patients (72.7%) were normal. In patients with DES, preoperative TBUT was not different (p = 0.851), but OSDI score was significantly higher (p<0.001). Criteria A showed a sensitivity of 73.3%, specificity of 55.0%, and PPV of 38.0%. Criteria B and C excluded preoperative TBUT, which had no difference between the two groups, and set screening criteria with preoperative OSDI score alone. Criteria B (preoperative OSDI score of 19.6 points or more) showed a sensitivity of 75.0%, specificity of 60.6%, PPV of 41.7% and AUC of 0.739 (p < 0.001). Criteria C was consisted of 5 out of 12 OSDI items that showed significant differences between the two groups; blurred vision, reading, working with a computer, low humidity, and air conditioning. Criteria C (preoperative 5-item OSDI score of 24.4 points or more) was a better predictability, with a sensitivity of 75.0%, specificity of 71.3%, PPV of 49.5%, and AUC of 0.804 (p < 0.001). The AUC of Criteria C was significantly higher than that of Criteria B (p = 0.0037).

Conclusion

DES occurred after successful EDCR in 27.3% of patients, and an OSDI questionnaire helped to screen DES. The predictability could improve using the modified OSDI score which showed noticeable difference in five OSDI items before and after EDCR.
How alginate properties influence in situ internal gelation in crosslinked alginate microcapsules (CLAMs) formed by spray drying
Authors: Jeoh Tina , Wong Dana E. , Strobel Scott A. , Hudnall Kevin , Pereira Nadia R. , Williams Kyle A. , Arbaugh Benjamin M. , Cunniffe Julia C. , Scher Herbert B.
Published: February 2021

Alginates gel rapidly under ambient conditions and have widely documented potential to form protective matrices for sensitive bioactive cargo. Most commonly, alginate gelation occurs via calcium mediated electrostatic crosslinks between the linear polyuronic acid polymers. A recent breakthrough to form crosslinked alginate microcapsules (CLAMs) by in situ gelation during spray drying (“CLAMs process”) has demonstrated applications in protection and controlled delivery of bioactives in food, cosmetics, and agriculture. The extent of crosslinking of alginates in CLAMs impacts the effectiveness of its barrier properties. For example, higher crosslinking extents can improve oxidative stability and limit diffusion of the encapsulated cargo. Crosslinking in CLAMs can be controlled by varying the calcium to alginate ratio; however, the choice of alginates used in the process also influences the ultimate extent of crosslinking. To understand how to select alginates to target crosslinking in CLAMs, we examined the roles of alginate molecular properties. A surprise finding was the formation of alginic acid gelling in the CLAMs that is a consequence of simultaneous and rapid pH reduction and moisture removal that occurs during spray drying. Thus, spray dried CLAMs gelation is due to calcium crosslinking and alginic acid formation, and unlike external gelation methods, is insensitive to the molecular composition of the alginates. The ‘extent of gelation’ of spray dried CLAMs is influenced by the molecular weights of the alginates at saturating calcium concentrations. Alginate viscosity correlates with molecular weight; thus, viscosity is a convenient criterion for selecting commercial alginates to target gelation extent in CLAMs.
The anurans and squamates assemblage from Final Natufian Eynan (Ain Mallaha, Israel) with an emphasis on snake-human interactions
Authors: Biton Rebecca , Bailon Salvador , Birkenfeld Michal , Bridault Anne , Khalaily Hamoudi , Valla François R. , Rabinovich Rivka
Published: February 2021

During the Natufian period, more than 12,000 years ago, Eynan (Ain Mallaha) was an important human settlement in the Hula Valley, Israel. This study concentrates on the anuran and squamate assemblage from the ultimate stage of the Natufian period at the site, the Final Natufian. Over five thousand bones assigned to at least sixteen taxa were studied from a sampled segment of the excavated open-air site. Relative species abundance, spatial distribution, taphonomic observations and ecological considerations all pointed to the conclusion that the inhabitants of Eynan intensively exploited three large “colubrine” snakes species: the Large Whip Snake (Dolichophis jugularis), the Eastern Montpellier Snake (Malpolon insignitus) and an Eastern Four-lined Ratsnake (Elaphecf.sauromates). These snakes were the most desired and were intensively gathered, while other snakes and lizards could have been opportunistically collected when encountered. We raise questions about whether the large “colubrines” exploitation should be interpreted as additional evidence of increasing diet breadth. We suggest challenging this line of reasoning and offer possible alternative motives.
Current status of Schistosoma mansoni infection among previously treated rural communities in the Abbey and Didessa Valleys, Western Ethiopia: Implications for sustainable control
Authors: Assefa Alemayehu , Erko Berhanu , Gundersen Svein Gunnar , Medhin Girmay , Berhe Nega
Published: February 2021

Background

Schistosoma constitutes a major public health problem and developmental challenges in the majority of developing and subtropical regions. The World Health Organization has set guidelines for the control and elimination of schistosomiasis. Ethiopia is providing school-based Mass Drug Administration (MDA) at the study areas of the Abbey and Didessa Valleys of western Ethiopian since 2015. Moreover, mass treatment was already done in the same villages 30 years ago. However, the current Schistosoma mansoni infection status among humans and snails in the study areas is not known. Hence, the present study aims to determine the current status.

Methods

A community-based cross-sectional study was conducted in the three communities; Chessega, Agallu Metti and Shimala in Schistosoma mansoni endemic areas of the Abbey and Didessa valleys in Western Ethiopia. Using the list of households obtained from the Kebele administration, a systematic sampling technique was used to select households in each village.

Results

Even though the area is under the Ethiopian national Mass Drug Administration campaign, the present study reports prevalence above 50%. Although the majority of the infections were moderate, we found that 13% had heavy infection, above 400 eggs per gram of stool, which is at the same level as before the treatment campaign 30 years ago. The infection was significantly higher among those below 12 years of age, among non-attending school-age children and daily laborers.

Conclusion

Schistosoma mansoni infection is still a public health problem in the study areas, despite control efforts already 30 years ago and present mass treatment in the last years. We suggest making the mass treatment campaign just early after the rainy season, when the snails are washed away. This should be supplemented with provisions of clean water, sanitation, and hygiene (WASH) and reduction of water contact and possible snail control efforts’ to prevent reinfection.
Knowledge, attitude and practice of vaccinators and vaccine handlers on vaccine cold chain management in public health facilities, Ethiopia: Cross-sectional study
Authors: Mohammed Solomon Ahmed , Workneh Birhanu Demeke , kahissay Mesfin Haile
Published: February 2021

Background

Effective management of the vaccine cold chain system at all levels is one of the crucial factors for maintaining vaccine potency. Vaccines require more complex handling and storage requirements due to increased temperature sensitivity and complicated immunization schedules. This urges adequate knowledge, attitude, and practice. This study assessed the knowledge, attitude, and practice of vaccinators and vaccine handlers’ in public health facilities.

Methodology

An institutional-based cross-sectional study design was used to assess the knowledge, attitude, and practice of 127 vaccinators and vaccine handlers in public health facilities of Oromia Special Zone, from September 1 to 30, 2019. Data were collected using self-administered questionnaires and a structured observation checklist. Descriptive and inferential statistics were made using the statistical package for social sciences version 20. Variables with a p-value <0.05 were taken as statistically significant.

Result

The response rate was (96.94%). Sixty-eight (53.5%; 95% CI: 46.5%, 61.4%), 58 (45.7%; 95% CI: 37.8%, 53.5%) and 62 (48.8%: 95% CI; 41.7%, 56.7%) vaccinators and vaccine handlers had satisfactory knowledge, positive attitude and good practice respectively. Receiving training on cold chain management had a statistically significant association with the level of knowledge on cold chain management (AOR = 3.04, 95% CI: 1.04–8.88).

Conclusions

More than half of vaccinators and vaccine handlers had satisfactory knowledge, while below half of vaccinators and vaccine handlers had a positive attitude and good practice. The determinants of knowledge in cold chain management were receiving training on cold chain management. Providing regular technical support and on the job training on vaccine cold chain management will improve the knowledge, attitude, and practice of vaccinators and vaccine handlers.
Chronic Disease in the Community (CDCom) Program: Hypertension and non-communicable disease care by village health workers in rural Uganda
Authors: Stephens Joseph H. , Addepalli Aravind , Chaudhuri Shombit , Niyonzima Abel , Musominali Sam , Uwamungu Jean Claude , Paccione Gerald A.
Published: February 2021

Background

Although hypertension, the largest modifiable risk factor in the global burden of disease, is prevalent in sub-Saharan Africa, rates of awareness and control are low. Since 2011 village health workers (VHWs) in Kisoro district, Uganda have been providing non-communicable disease (NCD) care as part of the Chronic Disease in the Community (CDCom) Program. The VHWs screen for hypertension and other NCDs as part of a door-to-door biannual health census, and, under the supervision of health professionals from the local district hospital, also serve as the primary providers at monthly village-based NCD clinics.

Objective/Methods

We describe the operation of CDCom, a 10-year comprehensive program employing VHWs to screen and manage hypertension and other NCDs at a community level. Using program records we also report hypertension prevalence in the community, program costs, and results of a cost-saving strategy to address frequent medication stockouts.

Results/Conclusions

Of 4283 people ages 30–69 screened for hypertension, 22% had a blood pressure (BP) ≥140/90 and 5% had a BP ≥ 160/100. All 163 people with SBP ≥170 during door-to-door screening were referred for evaluation in CDCom, of which 91 (59%) had repeated BP ≥170 and were enrolled in treatment. Of 761 patients enrolled in CDCom, 413 patients are being treated for hypertension and 68% of these had their most recent blood pressure below the treatment target. We find: 1) The difference in hypertension prevalence between this rural, agricultural population and national rates mirrors a rural-urban divide in many countries in sub-Saharan Africa. 2) VHWs are able to not only screen patients for hypertension, but also to manage their disease in monthly village-based clinics. 3) Mid-level providers at a local district hospital NCD clinic and faculty from an academic center provide institutional support to VHWs, stream-line referrals for complicated patients and facilitate provider education at all levels of care. 4) Selective stepdown of medication doses for patients with controlled hypertension is a safe, cost-saving strategy that partially addresses frequent stockouts of government-supplied medications and patient inability to pay. 5) CDCom, free for village members, operates at a modest cost of 0.20 USD per villager per year. We expect that our data-informed analysis of the program will benefit other groups attempting to decentralize chronic disease care in rural communities of low-income regions worldwide.
Lysis of arterial thrombi by perfusion of N,N’-Diacetyl-L-cystine (DiNAC)
Authors: Kim Dongjune , Shea Susan M. , Ku David N.
Published: February 2021

The search persists for a safe and effective agent to lyse arterial thrombi in the event of acute heart attacks or strokes due to thrombotic occlusion. The culpable thrombi are composed either primarily of platelets and von Willebrand Factor (VWF), or polymerized fibrin, depending on the mechanism of formation. Current thrombolytics were designed to target red fibrin-rich clots, but may be not be efficacious on white VWF-platelet-rich arterial thrombi. We have developed an in vitro system to study the efficacy of known and proposed thrombolytic agents on white clots formed from whole blood in a stenosis with arterial conditions. The agents and adjuncts tested were tPA, ADAMTS-13, abciximab, N-acetyl cysteine, and N,N’-Diacetyl-L-cystine (DiNAC). Most of the agents, including tPA, had little thrombolytic effect on the white clots. In contrast, perfusion of DiNAC lysed thrombi as quickly as 1.5 min, which ranged up to 30 min at lower concentrations, and resulted in an average reduction in surface area of 71 ± 20%. The clot burden was significantly reduced compared to both tPA and a saline control (p<0.0001). We also tested the efficacy of all agents on red fibrinous clots formed in stagnant conditions. DiNAC did not lyse red clots, whereas tPA significantly lysed red clot over 48 h (p<0.01). These results lead to a novel use for DiNAC as a possible thrombolytic agent against acute arterial occlusions that could mitigate the risk of hyper-fibrinolytic bleeding.
Menstrual hygiene management practices and associated health outcomes among school-going adolescents in rural Gambia
Authors: Nabwera Helen M. , Shah Vishna , Neville Rowena , Sosseh Fatou , Saidykhan Mariama , Faal Fatou , Sonko Bakary , Keita Omar , Schmidt Wolf-Peter , Torondel Belen
Published: February 2021

Inadequate menstrual hygiene management (MHM) practices have been associated with adverse health outcomes. This study aimed to describe MHM practices among schoolgirls from rural Gambia and assess risk factors associated with urogenital infections and depressive symptoms. A cross-sectional study was conducted among adolescent schoolgirls in thirteen schools in rural Gambia. A questionnaire was used to collect information on socio-demographics, MHM practices and clinical symptoms of reproductive and urinary tract infections (UTIs). A modified Beck Depression Inventory-II was used to screen for depressive symptoms. Mid-stream urine samples were collected to assess for UTIs. Modified Poisson regression analysis was used to determine risk factors for symptoms of urogenital infections and depression among adolescent girls. Three hundred and fifty-eight girls were recruited. Although, 63% of the girls attended schools providing free disposable pads, reusable cloths/towels were the commonest absorbent materials used. Heavy menstrual bleeding was associated with depressive symptoms (adjusted prevalence ratio, aPR 1.4 [95% CI 1.0, 1.9]), while extreme menstrual pain (aPR 1.3 [95% CI 1.2, 1,4]), accessing sanitary pads in school (aPR 1.4 [95% CI 1.2, 1.5]) and less access to functional water source at school (aPR 1.4 [95% CI 1.3, 1.6]) were associated with UTI symptoms. Conversely, privacy in school toilets (aPR 0.6 [95% CI 0.5, 0.7]) was protective for UTI symptoms. Heavy menstrual bleeding (aPR 1.4 [95% CI 1.1, 2.0]) and taking <30 minutes to collect water at home were associated with RTI symptoms (aPR 1.2 [95% CI 1.0, 1.5]) while availability of soap in school toilets (aPR 0.6 [95% CI 0.5, 0.8] was protective for RTI symptoms. Interventions to ensure that schoolgirls have access to private sanitation facilities with water and soap both at school and at home could reduce UTI and RTI symptoms. More attention is also needed to support girls with heavy menstrual bleeding and pain symptoms.
Validation of a combined approach-avoidance and conditioned stimulus aversion paradigm for evaluating aversion in chickens
Authors: du Plessis Emmarentia W. , Beausoleil Ngaio J. , Bolwell Charlotte F. , Stafford Kevin J.
Published: February 2021

Understanding animals’ aversion is important to improving their welfare. Aversion is often assessed using an approach-avoidance (AA) test in which animals have to forfeit a reward if they want to avoid an event or environment presented in the same place. However, sometimes the event/environment suspected to be aversive may physically impair the animal’s ability to withdraw from that place (i.e. its ability to express aversion), leading to incorrect interpretations. Combining AA with a Conditioned-Stimulus that predicts the event/environment may overcome this problem by allowing animals to demonstrate aversion without exposure to the stimulus. We aimed to validate this paradigm for testing aversion in chickens. Seven Hyline-Brown chickens were trained to obtain a food reward from a coloured bowl located in the test chamber (TC) of a two-chambered box; the reward was presented in a green bowl with an inactivated air canister or a red bowl with the canister activated to deliver an air puff. Two 5-minute tests were conducted, one with each bowl colour and both with the canister inactivated. All chickens entered TC with the green bowl. With the red bowl, two chickens entered on their first attempt, one fully entered after a partial entry (3/7 fully entered), two made only partial entries and two made no attempts to enter. Chickens spent less time in the TC with the red bowl (median 31s, IQR 7–252) compared to the green bowl (293s, IQR 290–294; p = 0.008). The higher ratio of partial to full entries, failure to enter the TC and less time spent in TC reflected chickens’ aversion to the air puff, signalled by the red bowl. The paradigm allowed chickens to demonstrate aversion without exposure to the aversive stimulus during testing.
Effect of a high crude protein content diet during energy restriction and re-alimentation on animal performance, skeletal growth and metabolism of bone tissue in two genotypes of cattle
Authors: Silva Tiago A. C. C. , Quigley Simon P. , Kidd Lisa J. , Anderson Stephen T. , McLennan Stuart R. , Poppi Dennis P.
Published: February 2021

The objective of this study was to investigate the effect of diet crude protein (CP) content and metabolisable energy (ME) intake on skeletal growth and associated parameters of growing steers prior to and during compensatory growth in weight and catch-up growth in skeletal elongation. The experiment was a factorial design with two cattle genotypes [Brahman crossbred (BX, 178 ± 6 kg) and Holstein-Friesian (HF, 230 ± 34 kg)] and three nutritional treatments; high CP content and high ME intake (HCP-HME), high CP content and low ME intake (HCP-LME) and low CP content and low ME intake (LCP-LME) with the ME intake of HCP-LME matched to that of LCP-LME. Nutritional treatments were imposed over a 103 d period (Phase 1), and after this, all steers were offered ad libitum access to the HCP-HME nutritional treatment for 100 d (Phase 2). Steers fed the high CP content treatment with a low ME intake, showed higher hip height gain (P = 0.04), larger terminal hypertrophic chondrocytes (P = 0.02) and a higher concentration of total triiodothyronine in plasma (P = 0.01) than steers with the same ME intake of the low CP content treatment. In addition, the low CP treatment resulted in significant decreases in bone volume (P = 0.03), bone surface area (P = 0.03) and the concentration of bone-specific alkaline phosphatase in plasma (P < 0.001) compared to steers fed the HCP-HME treatment. A significant interaction between genotype and nutritional treatment existed for the concentration of thyroxine (T4) in plasma where HF steers fed LCP-LME had a lower T4 concentration in plasma (P = 0.05) than BX steers. All steers with a restricted ME intake during Phase 1 demonstrated compensatory growth during Phase 2. However, HF steers fed the LCP treatment during Phase 1 showed a tendency (P = 0.07) for a greater LWG during Phase 2 without any increase in dry matter intake. Results observed at the growth plate and hip height growth suggest that catch-up growth in cattle may also be explained by the growth plate senescence hypothesis. Contrary to our initial hypothesis, the results demonstrate that greater CP intake during ME restriction does not increase compensatory gain in cattle during re-alimentation.