bluuebunny/crossref_metadata_2025 huggingface dataset:
In Vitro Evaluation of Cytotoxic and Antitumor Activities of The Tamoxifen and Doxorubicin Combination on MCF-7 and BT-474 Breast Cancer Cell Lines
Authors: SUİÇMEZ Menderes , NAMALIR Gamze , ÖZDİL Hilal
Published: November 2023

The combination therapy of breast cancer has preferred for the patients to minimize possible side effects, drug resistance, recurrence and toxic effects. In this study, we aim to investigate the cytotoxic and antitumor activities the tamoxifen and doxorubicin combination in breast cancer cell lines, MCF-7 and BT-474. Tamoxifen (Tam) and doxorubicin (Dox) and their combination with different concentrations (0.625–20 μM Tam; 0.0625–2 μM Dox and 5 μM Tam+ 0.5/1.0/1.5 μM Dox combination were applied to MCF-7 and BT-474 cells for 48 hours. Afterthat, their cytotoxic activities were analyzed with MTT assay. Bcl-2, Mapt and Mrp1 are genes that induce cell proliferation, inhibit apoptosis and play role in drug resistance in cancer cells. To evaluate the antitumor activities of these genes in combination treatment, mRNA levels were analyzed by quantitative PCR. According to the MTT assay, it was determined that IC50 values as 17.26 μM and 16.65 μM for tamoxifen on MCF-7 and BT-474 breast cancer cell lines. IC50 values of doxorubicin in MCF-7 and BT-474 cells were 1.65 μM and 1.57 μM, respectively. It was found that the application of the combination drugs (15 μM tamoxifen and 1.5 μM doxorubicin) in MCF-7 and BT-474 cells have the lowest combination index values as 1.09 and 1.26, respectively. Therefore, the combination of 15 μM tamoxifen and 1.5 μM doxorubicin was selected and applied to both breast cancer cell lines for gene expression analysis. It was found that while Mrp1 and Mapt genes expressions were significantly upregulated, Bcl-2 gene expression was downregulated in MCF-7 cells. However, Bcl-2, Mrp1 and Mapt genes expressions in BT-474 cells were not significantly regulated. Altogether, these findings suggest that the combination of these two drugs may have a potential antagonistic interaction according combination index values.
Atık Su Arıtma Tesislerinde Biyokimyasal Oksijen İhtiyacının Yapay Sinir Ağı ve Regresyon Analiziyle Tahmin Edilmesi
Authors: SİDAL Furkan , ALTUN Yener
Published: November 2023

Atık su arıtma tesislerinde su kalitesini takip edip müdahale etmek, tesislerin yönetiminde önemli bir rol oynar. Atık su arıtma tesisleri yapılırken ve işletilirken, biyolojik oksijen ihtiyacı değerlerine gereksinim duyulmaktadır. Bu değerin ölçülmesi diğer parametrelere göre daha uzun sürelerde gerçekleşmekte ve deneylerin yapılması da zahmetli ve maliyetli olmaktadır. Bu çalışmada biyolojik oksijen değerinin, atık su arıtma tesislerinde kolayca ölçülebilen diğer parametreler aracılığıyla yapay sinir ağları ve çoklu regresyon analizi teknikleriyle tahmin edilmesi amaçlanmıştır. Çalışmada kullanılan ölçüm sonuçları 2021-2022 yılları arasında Van iline ait bir atık su arıtma tesisinde ölçülen verileri kapsamaktadır. Kullanılan tahmin girdi parametreleri pH, elektriksel iletkenlik, sıcaklık, çözünmüş oksijen, kimyasal oksijen ihtiyacı, askıda katı madde, toplam azot ve toplam fosfor değerleri bağımsız değişken ve biyolojik oksijen değeri ise bağımlı değişken olarak seçilmiştir. Yapılan tahminlerde yapay sinir ağı modeli için MAPE değeri %0.12, MAD değeri 0.04, R değeri %99.83 ve R2 değeri %99.68 olarak elde edilmiştir. Aynı şekilde çoklu regresyon analizi yöntemi ile BOİ tahmin modelinde MAPE değeri %0.68, MAD değeri 0.06, R değeri %96.40 ve R2 değeri %92.92 olarak bulunmuştur. Çalışmada elde edilen bulgular biyolojik oksijen değerinin kolayca ölçülebilen parametreler yardımıyla ileri beslemeli yapay sinir ağları ve doğrusal çoklu regresyon analizi teknikleri ile oluşturulmuş olan modeller kullanılarak tahmin edilmesi mümkündür. Her iki model karşılaştırıldığında ise yapay sinir ağları ile geliştirilmiş olan modelin çoklu regresyon analizi ile geliştirilmiş olan modele göre daha iyi performans sergilediği tespit edilmiştir.
Agglomeration as a Strategic Track for the Regional Development: Prospects and Limitations
Authors: Seredyuk Ilya , Korchagina Irina
Published: November 2023

Abstract: Agglomeration is a traditional form of regional development. However, its place in the modern theory of strategizing has not been sufficiently studied. This research approached the Kemerovo Region as a socioeconomic object of strategizing. It relied on V.L. Kvint’s theory of strategy and methodology of strategizing, e.g., strategy laws, rules of strategic thinking, quantitative analysis of empirical data, etc. The new urban agglomerations need to be asymmetric and unpredictable for competitors, and their strategizing needs new approaches. The Kemerovo Region has two agglomerations, i.e., North Kuzbass and South Kuzbass, which are strategized as inherent parts of the region. In this case, the time-saving rule makes it possible to get ahead of those competitors who prefer more traditional approaches. The strategic priority of the regional agglomeration process possesses a number of competitive advantages, e.g., population density, road network, high urbanization, etc. The strategic project will eventually cover some territories that lack socio-economic ties. Another relevant rule of strategic thinking presupposes a certain commitment to the future, which makes it impossible to rely on the so-called everyday common sense. The Kemerovo Region chose a non-traditional agglomeration path, which ensures its compliance with the laws of strategy and the rules of strategic thinking
Strategizing the Interoperability between Scientific Thought Centers in Russia and Africa
Authors: Tishchenko Elena , Slavyantsev Maksim
Published: November 2023

The state accumulates national wealth only as fast as it transforms the existing scientific and technical reserves into economic resources, thus capitalizing natural resources into marketable goods and services. This research relied on a new approach to assessing national strength developed by V.L. Makarov, A.R. Bakhtizin, and E.D. Sushko. The approach is based on the presumption that strategizing labor potential ensures effective technological sovereignty, which makes science and innovations especially important. It presupposes the following basic principles of strategizing the labor potential development: legality, consistency, longterm, prolongation, continuity, humanity, freedom, and proportionality. To make these principles more effective, the authors substantiated the role of interoperability (compatibility) between science and innovations at technical, semantic, and organizational levels.
Modificaciones en la dieta y el cuidado del agua en el centro de la República Mexicana durante la pandemia de COVID-19
Authors: Kala-Saldaña Axel Roberto , Benítez-Aguilar Alexis , Espinosa Ayala Enrique , Márquez-Molina Ofelia , Velázquez Cigarroa Erasmo
Published: November 2023

La dieta desequilibrada impacta en el medioambiente a través de la agricultura, como un sector de alta emisión de gases de efecto invernadero (GEI) que usa el 70% de las reservas mundiales de agua. Además, existe una desigualdad en la distribución y uso de este recurso que se acrecentó durante el confinamiento por coronavirus. El objetivo de esta investigación fue identificar los cambios en la dieta y el cuidado del agua en la zona centro de México durante la pandemia de COVID-19. Encontrándose un aumento en el consumo de verduras (71.7%) y frutas (64.9%). En el cuidado del agua en casa la atención de fugas (58.4%) y regulación del tiempo de baño en regadera (54.5%) mostraron un incremento. Así mismo, se identificaron grupos caracterizados por acciones dirigidas a una alimentación adecuada y menos contaminante, un mejor cuidado del agua y la percepción de beneficios en la participación del cuidado del medio ambiente. La emisión de CO2 semanal de la dieta permitió identificar características del consumo de alimentos durante pandemia y una participación hacia mejorar el ecosistema. Dando bases para plantear estrategias que partan de las actividades que llevan a cabo las personas o que fomenten una mayor incidencia de estas.
Jürgen Habermas sobre a educação
Authors: Baumgartem França Bárbara Rebecca , Vidal da Silva Nazareth , Lima de Almeida Mariangela
Published: November 2023

Este artigo tem como objetivo apresentar um diálogo entre a literatura que perpassa a temática entre os filósofos Jürgen Habermas e Immanuel Kant, e suas contribuições para pensarmos o campo da Educação. Caracteriza-se por um estudo bibliográfico, fundamentado na análise e interpretação da temática proposta. Aponta-se algumas aproximações relevantes da perspectiva habermasiana e o pensamento kantiano, sobretudo no que diz respeito a conceitos básicos como emancipação e conhecimento, apropriando-se dos sentidos e interpretações discutidas nas obras bibliográficas elencadas. A partir do diálogo exposto, compreende-se as contribuições dos pressupostos comunicativos no campo educacional, na área de formação continuada de profissionais da educação, conduzindo assim a uma justiça social expressa por processos de ensino-aprendizagem, garantindo o preparo de profissionais da educação fundamentada na concepção de um professor reflexivo, pesquisador e que aprende com o coletivo, visando o atendimento às necessidades de seus estudantes.
Um caso de “homens infames”
Authors: Pagliarini Mariângela Nascimento , Lima Marcia Heloisa Tavares de Figueredo
Published: November 2023

Estudo bibliográfico, teórico-exploratório sobre memória institucional e sua complexa relação com a memória histórica nacional. Teve por objetivo geral contribuir para a reflexão acerca do modo como a biblioteca do Tribunal de Justiça Militar do Rio Grande do Sul poderia analisar documentos sensíveis para organizar, preservar e disseminar as informações contidas nos processos judiciais históricos atinentes a fatos ocorridos de 1964 a 1985 de forma a torná-las disponíveis para pesquisas futuras. Apresenta breve reflexão sobre memória institucional e sua tensão frente à memória/história. Mostra, como exemplos de processos judiciais sensíveis relevantes, os que foram instaurados durante o regime de exceção de 1964, e alcançaram os chamados grupos “Os Onze da Brigada”, utilizando a metáfora dos “homens infames” de Michel Foucault. Por fim, postula a pertinência de manutenção deste tipo de processo para evitar o apagamento das informações e proporcionar futuras pesquisas. Reconhece lacunas não respondidas acerca da organização propriamente dita deste acervo, motivadas pelo período de fechamento das instituições no contexto da COVID-19, pandemia mundial, e aponta para a conveniência de prosseguimento da pesquisa ou novas pesquisas no rico acervo.
democracia segundo Niklas Luhmann
Authors: Preger Guilherme
Published: November 2023

Este artigo apresenta a concepção de democracia adotada pela Teoria dos Sistemas Sociais na versão de Niklas Luhmann. Embora seja um tema pouco frequente na obra do sociólogo, ela foi exposta com clareza no artigo The Future of Democracy, publicado em 1990. Neste artigo, a concepção é apresentada como uma hipótese histórica que foi capaz de prever e explicar algumas características da democracia ocidental após a queda do bloco soviético. O artigo avança um desenvolvimento dessa concepção democrática para entender melhor o ressurgimento dos movimentos políticos de extrema-direita na última década e, por outro lado, mostrar como tais movimentos são incompatíveis com o princípio democrático proposto pela teoria luhmanniana.
Colóquios Habermas à luz do regime de informação
Authors: Freire Isa Maria
Published: November 2023

Compartilha resultados de pesquisa exploratória para aplicação do modelo de regime de informação ao conjunto das 14 edições dos Anais do Colóquio Habermas, evento de comunicação científica na área de Ciência da Informação. Trata-se de pesquisa descritiva e documental, apoiada no método indiciário (Ginzburg) e na técnica do brauseio (Araújo), realizada nos arquivos dos Anais dos Colóquios no período de 2009 a 2022. Adota a abordagem do regime de informação na perspectiva de González de Gómez, apresentando seus elementos — atores sociais, ações, dispositivos e artefatos de informação — no contexto da forma de vida da comunidade científica. Descreve as interações entre os elementos do regime de informação do Colóquio Habermas desde a criação, por atores sociais, de um valor de informação (estrato semântico-pragmático) à conformação das regras em dispositivos (estrato meta-informacional) e o compartilhamento dos resultados mediante artefatos digitais (infraestruturas de informação). Foram identificados quatro grupos de atores sociais no conjunto de participantes dos Colóquios Habermas: (a) os que participam desde o início; (b) os que começaram a participar em meados da série histórica; (c) os que começaram a participar recentemente, mantendo regularidade na participação; e (d) os que participaram apenas uma vez. Em uma análise preliminar, entende-se que através da realização dos Colóquios e da publicação dos Anais os resultados da pesquisa científica foram compartilhados e discutidos por uma comunidade qualificada, propiciando a criação de novos conhecimentos na Ciência da Informação.
Concentração e dispersão da formação dos pesquisadores da gestão do conhecimento
Authors: Silva Edcleyton Bruno Fernandes
Published: November 2023

Este trabalho objetivou analisar a formação dos pesquisadores da Gestão do Conhecimento, essencialmente os da Ciência da Informação no Brasil, a partir do estabelecimento de relações de concentração e dispersão das formações dos pesquisadores. Para tanto, foram considerados artigos indexados na Base de Dados Referenciais de Artigos de Periódicos em Ciência da Informação nos últimos cinco anos e verificação dos currículos lattes dos pesquisadores. A metodologia adotada foi de caráter quali-quantitativo, por meio de pesquisa tipo bibliográfica, exploratória e descritiva. Concluiu que a Gestão do Conhecimento é uma área que engloba diversas áreas do conhecimento e formações multifacetadas entre os pesquisadores, e que no Brasil, no âmbito da Ciência da Informação, verifica-se maior número de produções científicas voltadas para a temática, na área de Ciências Sociais Aplicadas. Acredita-se que a investigação pode contribuir para a identificação de tendências e relacionamentos entre outros domínios do saber, a partir do estabelecimento de relações de concentração e dispersão das formações dos pesquisadores de Gestão do Conhecimento.
problema da inteligência artificial aplicada às fotografias e o pensamento de Vilén Flusser
Authors: Guerra Claudia Bucceroni , Marques Junior Jairo André
Published: November 2023

Esta apresentação tem como objetivo iniciar a reflexão, no contexto teórico da Ciência da Informação, dos questionamentos que possam surgir e as possibilidades teóricas para responder tais questionamentos em relação à Inteligência Artificial aplicada à fotografia. A metodologia utilizada nesse estudo foi o levantamento bibliográfico sobre fotografia e IA nas áreas que dialogam de forma interdisciplinar com a Ciência da Informação, bem como o retorno às teorias de Vilén Flusser como aporte para possíveis interpretações favoráveis ao debate conceitual aqui proposto.
Anti-Gene IGF-I Vaccines in Cancer Gene Therapy: A Review of a
Case of Glioblastoma
Authors: Trojan Annabelle , Lone Yu-Chun , Briceno Ignacio , Trojan Jerzy
Published: November 2023

Objective:

Vaccines for the deadliest brain tumor - glioblastoma (GBM) - are
generally based on targeting growth factors or their receptors, often using antibodies.
The vaccines described in the review were prepared to suppress the principal cancer
growth factor - IGF-I, using anti-gene approaches either of antisense (AS) or of triple helix
(TH) type. Our objective was to increase the median survival of patients treated with
AS and TH cell vaccines.

Methodology:

The cells were transfected in vitro by both constructed IGF-I AS and
IGF-I TH expression episomal vectors; part of these cells was co-cultured with plant
phytochemicals, modulating IGF-I expression. Both AS and TH approaches completely
suppressed IGF-I expression and induced MHC-1 / B7 immunogenicity related to the
IGF-I receptor signal.

Results:

This immunogenicity proved to be stronger in IGF-I TH than in IGF-I AS-prepared
cell vaccines, especially in TH / phytochemical cells. The AS and TH vaccines
generated an important TCD8+ and TCD8+CD11b- immune response in treated GBM patients
and increased the median survival of patients up to 17-18 months, particularly using
TH vaccines; in some cases, 2- and 3-year survival was reported. These clinical results
were compared with those obtained in therapies targeting other growth factors.

Conclusion:

The anti-gene IGF-I vaccines continue to be applied in current GBM personalized
medicine. Technical improvements in the preparation of AS and TH vaccines to
increase MHC-1 and B7 immunogenicity have, in parallel, allowed to increase in the median
survival of patients.
Prediction of Human Microbe-Drug Association based on Layer
Attention Graph Convolutional Network
Authors: Qu Jia , Ni Jie , Ni Tong-Guang , Bian Ze-Kang , Liang Jiu-Zhen
Published: November 2023

:

Human microbes are closely associated with a variety of complex diseases and
have emerged as drug targets. Identification of microbe-related drugs is becoming a key
issue in drug development and precision medicine. It can also provide guidance for solving
the increasingly serious problem of drug resistance enhancement in viruses.

Methods:

In this paper, we have proposed a novel model of layer attention graph convolutional
network for microbe-drug association prediction. First, multiple biological data
have been integrated into a heterogeneous network. Then, the heterogeneous network has
been incorporated into a graph convolutional network to determine the embedded microbe
and drug. Finally, the microbe-drug association scores have been obtained by decoding
the embedding of microbe and drug based on the layer attention mechanism.

Results:

To evaluate the performance of our proposed model, leave-one-out crossvalidation
(LOOCV) and 5-fold cross-validation have been implemented on the two datasets
of aBiofilm and MDAD. As a result, based on the aBiofilm dataset, our proposed
model has attained areas under the curve (AUC) of 0.9178 and 0.9022 on global LOOCV
and local LOOCV, respectively. Based on aBiofilm dataset, the proposed model has attained
an AUC value of 0.9018 and 0.8902 on global LOOCV and local LOOCV, respectively.
In addition, the average AUC and standard deviation of the proposed model for 5-
fold cross-validation on the aBiofilm and MDAD datasets were 0.9141±6.8556e-04 and
0.8982±7.5868e-04, respectively. Also, two kinds of case studies have been further conducted
to evaluate the proposed models.

Conclusion:

Traditional methods for microbe-drug association prediction are timeconsuming
and laborious. Therefore, the computational model proposed was used to predict
new microbe-drug associations. Several evaluation results have shown the proposed
model to achieve satisfactory results and that it can play a role in drug development and
precision medicine.
Periodontitis Continuum: Antecedents, Triggers, Mediators, and Treatment Strategies
Authors: Gasmi Benahmed Asma , Tippairote Torsak , Gasmi Amin , Noor Sadaf , Avdeev Oleksandr , Shanaida Yurii , Mojgani Naheed , Emadali Alireza , Dadar Maryam , Bjørklund Geir
Published: November 2023

:

Periodontitis (PD) is a chronic inflammatory disease of the periodontium characterized
by the formation of gingival pockets and gingival recession. The local inflammatory
environment can lead to the destruction of the extracellular matrix and subsequent
bone loss. The pathophysiology of PD involves interactions between genetic predisposition,
lifestyle, environmental factors, the oral microbiota condition, systemic
health disorders, innate and adaptive immune responses, and various host defenses. The
review highlighted the importance of the oral cavity condition in systemic health. Thus,
a correlation between harmful oral microbiota and cardiovascular disease (CVD)/diabetes/
arthritis, etc, progressions through inflammation and bacterial translocation was
highlighted. Antecedents increase an individual's risk of developing PD, trigger initiate
microbe-host immunologic responses, and mediators sustain inflammatory interactions.
Generally, this review explores the antecedents, triggers, and mediators along the pathophysiological
continuum of PD. An analysis of modern approaches to treating periodontitis,
including antibiotics for systemic and local use, was carried out. The potential role
of natural ingredients such as herbal extracts, phytoconstituents, propolis, and probiotics
in preventing and treating PD was highlighted.
Identification of an Exosome-relevant SNHG6-hsa-miR-429-
CHRDL1/CCNA2 Axis for Lung Adenocarcinoma Prognosis
Evaluation
Authors: Yuan Yuan , Li Qian , Chen Feifei , Zhao Yujie , Ma Jiyong , Fang Surong
Published: November 2023

Aims:

To explore an exosome-relevant molecular classification in lung adenocarcinoma (LUAD).

Background:

Exosome genes or relevant non-coding RNAs are regulators of cancer
treatment and prognosis, but their function in LUAD has not yet been determined.

Objective:

Unraveling a molecular classification applying exosome-related RNA networks for LUAD prognosis evaluation.

Methods:

MicroRNA sequencing data (miRNAs-seq) and RNA sequencing data (RNA-
seq) were derived from The Cancer Genome Atlas (TCGA). The ConsensusCluster-
Plus package was used for molecular typing in LUAD based on 121 Exosome-related
genes. Then, a limma package was conducted to explore differentially expressed mRNAs
(DEmRNAs), differentially expressed miRNAs (DEmiRNAs) and differentially expressed
lncRNAs (DElncRNAs) in molecular typing for constructing an Exosome-driven
competing endogenous RNA network (ceRNA). Dominant miRNAs, as well as target
mRNAs, were identified by COX modeling and Kaplan-Meier survival analysis.

Results:

Two Exosome-associated molecular clusters classified in LUAD. The C2 cluster
favored high clinicopathology and showed a trend toward poor prognosis. 29 lncRNA-
miRNA and 12 miRNA-mRNA interaction pairs were identified. The hsa-miR-429
was the pivotal miRNA in the network that affected the prognosis of LUAD. According
to the interaction relationship and LUAD prognostic role, SNHG6-hsa-
miR-429-CHRDL1/CCNA2 was identified. SNHG6-hsa-miR-429-CHRDL1 exerts
oncogenic effects, and SNHG6-hsa-miR-429- CCNA2 exerts pro-oncogenic effects.

Conclusion:

Overall, our study identified an Exosome-driven ceRNA network in LUAD,
and the SNHG6-hsa-miR-429-CHRDL1/CCNA2 axis could be a new therapeutic
target for LUAD and our study provides new insights into the molecular mechanisms of
LUAD.
Design, Molecular Modeling, MD Simulations, Essential Dynamics, ADMET, DFT,
Synthesis, Anti-proliferative, and Apoptotic Evaluations of a New Anti-VEGFR-2
Nicotinamide Analogue
Authors: Eissa Ibrahim H. , Elkaeed Eslam B. , Elkady Hazem , Yousef Reda G. , Alsfouk Bshra A. , Elzahabi Heba S.A. , Ibrahim Ibrahim M. , Metwaly Ahmed M. , Husein Dalal Z.
Published: November 2023

Objectives:

This study aims to design and evaluate (in silico and in vitro) a new nicotinamide derivative
as an inhibitor of VEGFR-2, a major mediator of angiogenesis.

Methods:

The following in silico studies were performed; DFT calculations, molecular modelling, MD simulations,
MM-GBSA, PLIP, and PCAT studies. The compound's in silico (ADMET) analysis was also
conducted. Subsequently, the compound ((E)-N-(4-(1-(2-(4-(4-Chlorobenzamido)benzoyl)hydrazono)ethyl)
phenyl)nicotinamide) was successfully synthesized and designated as compound X. In vitro, VEGFR-2 inhibition
and cytotoxicity of compound X against HCT-116 and A549 cancer cell lines and normal Vero cell lines
were conducted. Apoptosis induction and migration assay of HCT-116 cell lines after treatment with compound
X were also evaluated.

Results:

DFT calculations assigned stability and reactivity of compound X. Molecular docking and MD simulations
indicated its excellent binding against VEGFR-2. Furthermore, MM-GBSA analysis, PLIP experiments,
and PCAT studies confirmed compound X’s correct binding with optimal dynamics and energy. ADMET
analysis expressed its general likeness and safety. The in vitro assays demonstrated that compound X
effectively inhibited VEGFR-2, with an IC50 value of 0.319 ± 0.013 μM and displayed cytotoxicity against
HCT-116 and A549 cancer cell lines, with IC50 values of 57.93 and 78.82 μM, respectively. Importantly,
compound X exhibited minimal toxicity towards the non-cancerous Vero cell lines, (IC50 = 164.12 μM). Additionally,
compound X significantly induced apoptosis of HCT-116 cell lines and inhibited their potential to
migrate and heal.

Conclusion:

In summary, the presented study has identified compound X as a promising candidate for the development
of a novel apoptotic lead anticancer drug.
Withaferin A Inhibits Liver Cancer Tumorigenesis by Suppressing
Aerobic Glycolysis through the p53/IDH1/HIF-1α Signaling Axis
Authors: Zhou Xiangyang , Wu Di , Zhu Linmiao , Li Ruohan , Yu Haitao , Li Wenjuan
Published: November 2023

Background:

The energy supply of certain cancer cells depends on aerobic glycolysis
rather than oxidative phosphorylation. Our previous studies have shown that withaferin A (WA), a
lactone compound derived from Withania somnifera, suppresses skin carcinogenesis at least partially
by stabilizing IDH1 and promoting oxidative phosphorylation. Here, we have extended our
studies to evaluate the anti-tumor effect of WA in liver cancer.

Methods:

Differential expression of glycolysis-related genes between liver cancer tissues and normal
tissues and prognosis were verified using an online database. Glycolysis-related protein expression
was detected using western blot after overexpression and knockdown of IDH1 and mitochondrial
membrane potential assay based on JC-1, and mitochondrial complex I activity was also
detected. The inhibitory effect of WA on the biological functions of HepG2 cells was detected
along with cell viability using MTT assay, scratch assay, clone formation assay, glucose consumption
and lactate production assay. Western blot and qRT-PCR were used to detect the expression
of proteins and genes related to IDH1, p53 and HIF1α signaling pathways.

Results:

We first identified that IDH1 expression was downregulated in human liver cancer cells
compared to normal liver cells. Next, we found that treatment of HepG2 cells with WA resulted in
significantly increased protein levels of IDH1, accompanied by decreased levels of several glycolytic
enzymes. Furthermore, we found that WA stabilized IDH1 proteins by inhibiting the degradation
by the proteasome. The tumor suppressor p53 was also upregulated by WA treatment, which
played a critical role in the upregulation of IDH1 and downregulation of the glycolysis-related
genes. Under hypoxic conditions, glycolysis-related genes were induced, which was suppressed
by WA treatment, and IDH1 expression was still maintained at higher levels under hypoxia.

Conclusion:

Taken together, our results indicated that WA suppresses liver cancer tumorigenesis
by p53-mediated IDH1 upregulation, which promotes mitochondrial respiration, thereby inhibiting
the HIF-1α pathway and blocking aerobic glycolysis.
An Updated Review on Molecular Biomarkers in Diagnosis and Therapy
of Colorectal Cancer
Authors: Ravichandran Shruthi Nagainallur , Kumar Makalakshmi Murali , Das Alakesh , Banerjee Antara , Veronica Suhanya , Sun-Zhang Alexander , Zhang Hong , Anbalagan Muralidharan , Sun Xiao-Feng , Pathak Surajit
Published: November 2023

Colorectal cancer is one of the most common cancer types worldwide. Since colorectal
cancer takes time to develop, its incidence and mortality can be treated effectively if it is detected
in its early stages. As a result, non-invasive or invasive biomarkers play an essential role in the early diagnosis of colorectal cancer. Many experimental studies have been carried out to assess genetic, epigenetic, or protein markers in feces, serum, and tissue. It may be possible to find biomarkers
that will help with the diagnosis of colorectal cancer by identifying the genes, RNAs, and/or proteins indicative of cancer growth. Recent advancements in the molecular subtypes of colorectal
cancer, DNA methylation, microRNAs, long noncoding RNAs, exosomes, and their involvement
in colorectal cancer have led to the discovery of numerous new colorectal cancer biomarkers. In
small-scale investigations, most biomarkers appear promising. However, large-scale clinical trials
are required to validate their effectiveness before routine clinical implementation. Hence, this review focuses on small-scale investigations and results of big data analysis that may provide an
overview of the biomarkers for the diagnosis, therapy, and prognosis of colorectal cancer.
Interaction of C-terminal Truncated Beta-amyloid Peptides with Human Serum Albumin
Authors: Firozabadi Diba Aslani , Bozorgmehr Mohammad Reza , Beyramabadi Safar Ali , Mohseni Sharareh
Published: November 2023

Background:

The formation of plaque from protein fibrils is the major source of diseases,
such as Alzheimer's and Prion diseases. Amyloid beta (Aβ) is a peptide with different lengths,
which is one of the main components of the plaque in the brain of people with Alzheimer's. Of the
amyloid beta of various lengths in the brain cells plaque, beta-amyloid with 40 amino acids (Aβ1-
40) is more abundant than the rest. Aβ monomers are in a dynamic equilibrium of various conformations
with beta sheets that aggregate as oligomers or larger structures. The misfolding of betaamyloid
peptide is involved in its accumulation. On the other hand, various species that exist in the
cell environment can affect the structure of beta-amyloid peptides.

Aims:

This study aimed to study the interaction of truncated forms of beta-amyloid peptide with
human albumin serum protein.

Objective:

Interaction of beta-amyloid peptide with other proteins is effective in causing Alzheimer's
disease. These include interactions between beta-amyloid and cell surface proteins, such as
prions and extracellular proteins, such as clusterins and human serum albumin (HSA). As HSA
concentrations are higher than other proteins, more than half of the interaction of beta-amyloid with
proteins is related to interaction with this protein. Interaction of HSA with beta-amyloid reduces the
aggregation of beta-amyloid. However, due to the diversity of beta-amyloid peptides with different
lengths, the mechanism of their interaction with HSA has not been well understood. In this work,
the interaction of C-terminal truncated beta-amyloid peptides with HSA has been investigated.

Method:

The C-terminal truncated forms of beta-amyloid peptides, Aβ1 − 26, Aβ1 − 30, and
Aβ1 − 36 and Aβ1 − 40, were designed in silico. Docking between these truncated peptides was
performed with serum albumin. A molecular dynamics simulation of the interaction of designed
peptides with serum albumin was also performed.

Results and Discussion:

The results showed that Aβ1 − 26 and Aβ1 − 30 peptides interact with the
interfacial region of the chains A and B of HSA and the surface of the HSA. While the interaction of
Aβ1 − 36 and Aβ1 − 40 peptides occurs only with the HSA surface. On the other hand, the interaction
of peptides with chain A of HSA is more favorable than their interaction with chain B of HSA.
Also, as the length of the peptide increases, the number of residues involved in the hydrophobic interaction
increases. The results of molecular dynamics simulation confirm the results obtained from
docking.

Conclusion:

The results of molecular dynamics and docking simulations show that the binding affinity
of peptides to serum albumin decreases with peptide shortening. Also, by changing the structure
of beta-amyloid peptides, serum albumin reduces their tendency to aggregate.
Immunomodulatory Effects of Clozapine: More Than Just a Side Effect in
Schizophrenia
Authors: Amerio Andrea , Magnani Luca , Arduino Gabriele , Fesce Fabio , de Filippis Renato , Parise Alberto , Costanza Alessandra , Nguyen Khoa D. , Saverino Daniele , De Berardis Domenico , Aguglia Andrea , Escelsior Andrea , Serafini Gianluca , De Fazio Pasquale , Amore Mario
Published: November 2023

Recent evidence suggests a possible relationship between the immune system and schizophrenia
spectrum disorders (SSDs), as neuroinflammation appears to play a role in major psychiatric
conditions. Neuroinflammation is as a broad concept representing a physiological protective response
to infection or injury, but in some cases, especially if chronic, it may represent an expression of maladaptive
processes, potentially driving to clinical dysfunction and neurodegeneration. Several studies
are concurrently highlighting the importance of microglia, the resident immune cells of the central
nervous system, in a huge number of neurodegenerative diseases, including multiple sclerosis, Alzheimer’s
and Parkinson’s diseases, as well as SSDs. A more fundamental phenomenon of maladaptive
coupling of microglia may contribute to the genesis of dysfunctional brain inflammation involved in
SSDs, from the onset of their neurophenomenological evolution. Clozapine and other antipsychotic
drugs seem to express a provable immunomodulant effect and a more specific action on microglia,
while neuroactive steroids and nonsteroidal anti-inflammatory drugs may reduce some SSDs symptoms
in add-on therapy. Given these theoretical premises, this article aims to summarize and interpret
the available scientific evidence about psychotropic and anti-inflammatory drugs that could express an
immunomodulant activity on microglia.
Respiratory System Examination
Authors: Ghaly Yousif Nasser , Hadi Najah R. , Alamran Fadhil G.
Published: November 2023

The respiratory examination is normally performed according to Osler's
classic sequence of inspection, palpation, percussion, and auscultation. All lobes of the
lung should be systematically examined. The examiner should be aware of the surface
projections of each of the five lobes. The present chapter is mainly focused on how to
apply physical examination of the respiratory system
The Anti-gender and Gender-critical Roots of the Italian Anti-trans Parent Activism
Authors: Prearo Massimo
Published: November 2023

Between 2020 and 2021, the fight against the hate crimes bill proposal on discrimination and violence based on sex, gender, sexual orientation, gender identity, and disability marked a pivotal moment in the ongoing mobilization against LGBTQ+ rights in Italy. Proposed by the main center-left party, the Bill proposal encountered a strong opposition both by right-wing and by MPs of the progressive area itself. In particular, the category of gender identity was targeted as a danger, a confused notion, a trojan horse of the “gender ideology”, etc. The Bill first adopted by the Lower House was later blocked at the Senate, thanks to the decisive action of Senator Simone Pillon, a founder and member of the Italian anti-gender movement, elected in 2018 with the radical right party of the League, led by Matteo Salvini. Anti-gender groups such as ProVita & Famiglia and Family Day, who had been campaigning against "gender theory" and "LGBT ideology" for over a decade, led this battle from outside the Parliament, online and in the streets, but also inside the Senate, during the parliamentary auditions to which they were massively invited to participate.
TERF and Anti-trans Initiatives in Russia: Is There a Connection?
Authors: Kirey-Sitnikova Yana
Published: November 2023

Trans-exclusive radical feminism (TERF) was imported onto the Russian soil around 2013, when trans people became more visible and I introduced transfeminism in a number of blog posts, which later were compiled into the first Russian-language book on transfeminism (Kirey-Sitnikova, 2016). In 2016, a large survey of feminists (n = 1364) revealed that 10.2% respondents identified as TERF and 4.4% as transfeminists (Kirey-Sitnikova, 2018). The TERF movement was shown to consist of individuals with higher income and educational level living in big cities, lesbians, and those more involved in feminist activism. Knowing a trans person was not a predictor of trans inclusion, many feminists joined the TERF movement after meeting a trans person supporting patriarchal gender norms (Kirey-Sitnikova, 2016). The most vigorous debates between these groups occurred on the Russian blogging platform “LiveJournal” (LJ) and consisted largely of translations and interpretations of texts by US feminists (Ibid.). As I described elsewhere, self-colonisation in the form of indiscriminate borrowing of terms and concepts from the Anglosphere is a prominent characteristic of all Russian progressive gender movements (Kirey-Sitnikova, 2020). After 2016, I moved on to other topics and did not follow these debates. This small contribution is aimed as an update on TERF from that time till now.
Composición taxonómica y afinidad por un sustrato de macroalgas marinas conspicuas del Parque Nacional Arrecifes de Cozumel, Quintana Roo, México
Authors: Carranza Ramirez Anahi , Calva Benítez Laura Georgina , Núñez Resendiz María Luisa
Published: November 2023

Antecedentes y Objetivos: Los arrecifes del Parque Nacional Arrecifes de Cozumel constituyen una de las principales formaciones arrecifales de México. Sin embargo, actualmente, son prácticamente inexistentes los estudios ficoflorísticos que permiten detectar cambios en la composición ficoflorística o su afinidad por algún sustrato. Por lo tanto, nuestro objetivo fue determinar la composición taxonómica de macroalgas marinas presentes en el Parque Nacional Arrecifes de Cozumel, así como realizar sus descripciones morfológicas y análisis de afinidad con el sustrato que colonizan.
Métodos: Los muestreos se realizaron en octubre y noviembre de 2018 en 14 arrecifes del Parque Nacional Arrecifes de Cozumel, mediante buceo autónomo. Se realizaron observaciones morfo-anatómicas utilizando tanto microscopios ópticos como estereoscópicos. La determinación taxonómica se realizó a partir de literatura especializada.
Resultados clave: Se determinaron 42 taxa: cinco Ochrophyta, 12 Rhodophyta y 25 Chlorophyta. La familia mejor representada para Ochrophyta fue Dictyotaceae; para Rhodophyta, Ceramiaceae y Rhodomelaceae; y para Chlorophyta, Halimedaceae. Se describen nuevos registros para el Parque Nacional Arrecifes de Cozumel incluyendo a Lobophora guadeloupensis como nuevo registro para México. Adicionalmente, se identificaron cuatro diferentes sustratos para las algas: psamofítico (33%), epilítico (32%), epizoico (19%) y epífito (16%). Los arrecifes más diversos fueron La Francesa (LF) y Santa Rosa (SR), y los menos diversos fueron Chankanaab (CK) y Tormentos (TM).
Conclusiones: Aunque muchos de los registros previos en el área fueron confirmados en el presente estudio, la presencia de 18 nuevos registros reveló, por un lado, la falta de monitoreos constantes de la riqueza algal de la región, y, por otro lado, la necesidad de contar con descripciones morfológicas o referencias fotográficas que permitan contrastar los registros previos con los actuales. Dada la importancia del Parque Nacional Arrecifes de Cozumel, nuestros resultados, además de ofrecer información actualizada, representan un punto de partida para estudios en biología de la conservación.
The Efficacy and Therapeutic Alliance of Augmented Reality Exposure Therapy in Treating Adults With Phobic Disorders: Systematic Review
Authors: Hasan Safa , Alhaj Hamid , Hassoulas Athanasios
Published: November 2023

Background

Phobic disorders are characterized by excessive fear of a stimulus that can affect the quality of a patient’s life. The lifetime prevalence in adults is 7.7% to 12.5%. The current literature provides evidence-based inferences about the effectiveness of in-vivo exposure therapy (IVET) in treating phobia. However, this method can put the therapist and the client in danger, with high drop out and refusal rates. A newer approach for exposure therapy using augmented reality technology is under assessment.

Objective

This systematic review investigated the novel technology’s efficacy, cost-efficacy, and therapeutic alliance in treating adults with phobia.

Methods

An extensive search was conducted using 4 major databases (MEDLINE, PsycINFO, Embase, and Scopus) using a comprehensive list of synonyms for augmented reality exposure therapy (ARET) and phobic disorders. The search targeted any randomized control trial testing ARET in adults with phobic disorders up to August 8, 2022.

Results

A total of 6 studies were included, with 208 participants providing results. Studies investigating the efficacy of ARET compared to no intervention showed significant results (P<.05) in the ARET group improvement. Head-to-head comparative studies comparing ARET to IVET showed no significant difference (P>.05) in the effectiveness and therapeutic alliance between both therapies. Further, the results demonstrated that the ARET group had a better long-term effect than IVET, with the ability to put the patients in more situations to face the feared object.

Conclusions

The current data suggest clinically significant efficacy and a promising therapeutic alliance of ARET. However, no data are available investigating the cost-effectiveness of ARET. Further research is warranted to ascertain ARET’s cost-effectiveness and examine its efficacy in other populations and anxiety conditions.
Combination of Curcuminoids and Acupressure for Inflammation and Pain in Older People with Osteoarthritis Genu: Protocol for a Randomized Controlled Trial (Preprint)
Authors: Mahanani Srinalesti , Kertia Nyoman , Madyaningrum Ema
Published: November 2023

BACKGROUND

Curcuminoids and acupressure have beneficial effects in reducing pain and inflammation in patients with osteoarthritis. However, only a few clinical trials are investigating biomarkers to prove this objectively.

OBJECTIVE

This study aims to investigate the effect of acupressure and curcuminoids on inflammatory markers and pain in older people with osteoarthritis genu.

METHODS

A randomized controlled trial (RCT) was conducted among older people with osteoarthritis. All participants were randomized to a group that received 30 mg of curcuminoids in turmeric extract capsules and acupressure (group 1) or a group that received a placebo and sham acupressure (group 2) for 3 weeks.

RESULTS

The study was approved by the research ethics board; ClinicalTrials.gov reviewed this protocol. The extracts were manufactured from May 2023 to June 2023. Participant recruitment was conducted in September and October 2023; a total of 72 participants aged 60 years or older participated, of whom 75% (n=54) were female. Data were analyzed in April 2024, and dissemination of results is expected by the end of 2024.

CONCLUSIONS

Primary outcomes were assessed at baseline and after the intervention. Relationships were assessed with inflammatory markers, endorphin hormones, and blood level of cycloxygenase-2 hormone. Additionally, secondary outcomes included pain, ability to perform activities of daily living, and quality of life. The beneficial effects that may be found in this trial may be exceptionally relevant in clinical practice, justifying this scientific inquiry. The benefits of herbs and acupressure can be helpful as additional options in treating inflammation and pain in patients with osteoarthritis.

CLINICALTRIAL

ClinicalTrials.gov NCT06105840; https://clinicaltrials.gov/study/NCT06105840

INTERNATIONAL REGISTERED REPORT

DERR1-10.2196/54970
Perceptions and Acceptance of Online Learning During the COVID-19 Pandemic: A Study Among Medical Students and Teachers (Preprint)
Authors: Ezzi Sohaib
Published: November 2023

BACKGROUND

The international recognition of the coronavirus pandemic (COVID-19) has prompted Chinese institutions to implement online learning. However, the actual responses of teachers and students to online education remain unknown.

OBJECTIVE

the current research was conducted to test the learning and teaching abilities during online teaching and learning. Moreover, estimating the physiological conditions and the difficulties facing teachers and students through the online classes during the pandemic

METHODS

: An online questionnaire survey was conducted, among medical students and teachers of Universities and colleges in Zhejiang. The questionnaires investigated the participants actual opinions on online learning and teaching.

RESULTS

A total of 1036 valid interviewees were involved in the present study, 478 males (46.1%) and 558 females (53.9%), including 155 teachers (15%) and 881 students (85%) (Chinese and international). The participants' age was divided into five categories 18 – 25 (62.2%), 26-30 (15.3%), 31-35 (12.7%), 36-40 (3.9%), and above 40 5.9%). The results reflected that teachers and students encouraged e-learning education during the pandemic as most of them could be adapted to the recent teaching style. They indicated that usage of electronic devices was very good (32.2%), and the internet connection during online classes was excellent, especially for Chinese students (32.5%). In addition, the majority of teachers and students responded to questions like “was the online communication easy and effective” was “yes” (54.20% teachers vs. 44.50% students), “what is the preferred method of communication during online learning” as “face-to-face communication (video call)” (43.90% teachers vs. 33.70% students), and “do you think learning or teaching the same as you were before the COVID-19 pandemic” as “somewhat” (49.70% teachers vs. 39.70% students). Furthermore, lockdown and quarantine were one of the concerns of the pandemic, especially for participants who traveled for their study (34.80% teachers vs. 25.20% students). On the other hand, there was a significant change in gender between Chinese and international interviewees as the Chinese females were more than international ones (58.9% Chinese vs. 45.7%) . In contrast, the international males were more than the Chinese ones (41.1% Chinese vs. 54.3% international). Additionally, international participants showed that the main struggle during the pandemic was the online classes (30.10% Chinese vs. 34.20% foreigners).

CONCLUSIONS

most instructors and students supported online education. Although teachers were less adaptive to online education, many still had favorable evaluations. The acceptability of online instruction was affected by sexual orientation.
Bumiayu dan Daya Tariknya dalam Karya Tulis Siswa-siswi SMA Muhammadiyah Bumiayu
Authors: Roselani Ni Gusti Ayu , Sutrisno Adi , Hardjanto Tofan Dwi , Sembodo Thomas Joko Priyo
Published: November 2023

High school is an important period for the development of one's personality. Positive or negative influences can easily be absorbed by teenagers at that age. This is considered a good opportunity for the PkM team of the English Study Program, Faculty of Cultural Sciences, Universitas Gadjah Mada to share knowledge with the students of SMA Muhammadiyah Bumiayu. With a mentoring approach (online & offline), the team invited the high school students to learn to write descriptive feature stories centered on the topic of tourist attractions around the Bumiayu District and the traditional culinary specialties of the area. The writings produced by the assisted students show good success from the mentoring system applied, but there are still some linguistic and organizational problems that need to be improved.

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Masa SMA adalah suatu periode penting bagi perkembangan kepribadian seseorang. Pengaruh positif ataupun negatif dengan mudah dapat diserap oleh remaja di usia tersebut. Hal ini dianggap sebagai kesempatan baik bagi tim PkM Program Studi (Prodi) Sastra Inggris, Fakultas Ilmu Budaya, Universitas Gadjah Mada untuk berbagi ilmu kepada siswa-siswa SMA Muhammadiyah Bumiayu. Dengan pendekatan pendampingan/mentoring dan sistem bauran (tatap muka online & tatap muka langsung), tim mengajak siswa-siswi SMA tersebut untuk belajar menulis cerita fitur yang bersifat deskriptif yang berpusat pada topik tempat wisata di sekitar wilayah Kecamatan Bumiayu dan kuliner tradisional khas daerah tersebut. Tulisan yang dihasilkan oleh siswa dampingan menunjukkan keberhasilan yang baik dari sistem mentoring yang diterapkan. Akan tetapi, masih terdapat beberapa masalah kebahasaan dan pengorganisasian yang harus diperbaiki.