bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Dietary Riboflavin Supplementation on the Growth Performance, Body Composition and Anti-Oxidative Capacity of Coho Salmon (Oncorhynchus kisutch) Post-Smolts
Authors: Yu Hai-Rui , Guo Meng-Jie , Yu Le-Yong , Li Ling-Yao , Wang Qing-He , Li Fang-Hui , Zhang Yu-Zhuo , Zhang Jia-Yi , Hou Ji-Yun
Published: November 2022

The present study investigated the effects of dietary riboflavin on growth performance, body composition and anti-oxidative capacity of coho salmon (Oncorhynchus kisutch) post-smolts. Seven experimental diets were formulated with graded riboflavin levels of 0.00, 3.96, 8.07, 16.11, 31.81, 63.67 and 126.69 mg/kg, respectively. Each diet was fed to triplicate groups of 10 fish with an individually initial mean body weight of 186.22 ± 0.41 g in 21 cages (water volume, 1000-L/cage) and fed three times daily (7:30, 12:30 and 17:30) to apparent satiation for 12 weeks. Fish fed a diet with 31.81 mg/kg riboflavin had the highest specific growth rate (SGR), which was significantly higher than fish-fed diets with 0.00, 3.96, 8.07 and 126.69 mg/kg riboflavin (p < 0.05). Feed conversion ratio showed an inverse trend with SGR. No significant differences were observed in condition factor, hepatosomatic index, viscerosomatic index, muscle moisture, crude protein and ash contents among dietary groups. Muscle lipid had the highest content in the 31.81 mg/kg group and was significantly higher (p < 0.05) than those in the 0.00, 3.96 and 8.07 mg/kg groups. The alanine aminotransferase, aspartate aminotransferase and malondialdehyde contents in the liver and serum of fish were significantly decreased with the increase in dietary riboflavin level up to 31.81 mg/kg, and then increased as dietary riboflavin level further increased. An inverse trend was observed for total superoxide dismutase and catalase activities. Serum total cholesterol and triglyceride levels were significantly decreased with the dietary of riboflavin levels up to 31.81 and 63.67 mg/kg, respectively. The cubic curve regression analysis based on SGR indicated that the optimum dietary riboflavin level was estimated to be 35.26 mg/kg for coho salmon post-smolts.
Coenzyme Q10: Role in Less Common Age-Related Disorders
Authors: Mantle David , Hargreaves Iain P.
Published: November 2022

In this article we have reviewed the potential role of coenzyme Q10 (CoQ10) in the pathogenesis and treatment of a number of less common age-related disorders, for many of which effective therapies are not currently available. For most of these disorders, mitochondrial dysfunction, oxidative stress and inflammation have been implicated in the disease process, providing a rationale for the potential therapeutic use of CoQ10, because of its key roles in mitochondrial function, as an antioxidant, and as an anti-inflammatory agent. Disorders reviewed in the article include multi system atrophy, progressive supranuclear palsy, sporadic adult onset ataxia, and pulmonary fibrosis, together with late onset versions of Huntington’s disease, Alexander disease, lupus, anti-phospholipid syndrome, lysosomal storage disorders, fibromyalgia, Machado-Joseph disease, acyl-CoA dehydrogenase deficiency, and Leber’s optic neuropathy.
Using Mobile Monitoring and Atmospheric Dispersion Modeling for Capturing High Spatial Air Pollutant Variability in Cities
Authors: Fattoruso Grazia , Toscano Domenico , Cornelio Antonella , De Vito Saverio , Murena Fabio , Fabbricino Massimiliano , Di Francia Girolamo
Published: November 2022

Air pollution is still one of the biggest environmental threats to human health on a global scale. In urban environments, exposure to air pollution is largely influenced by the activity patterns of the population as well as by the high spatial and temporal variability in air pollutant concentrations. Over the last years, several studies have attempted to better characterize the spatial variations in air pollutant concentrations within a city by deploying dense, fixed as well as mobile, low-cost sensor networks and more recently opportunistic sampling and by improving the spatial resolution of air quality models up to a few meters. The purpose of this work has been to investigate the use of properly designed mobile monitoring campaigns along the streets of an urban neighborhood to assess the capability of an operational air dispersion model as SIRANE at the district scale to capture the local variability of pollutant concentrations. To this end, an IoT ecosystem—MONICA (an Italian acronym for Cooperative Air Quality Monitoring), developed by ENEA, has been used for mobile measurements of CO and NO2 concentration in the urban area of the City of Portici (Naples, Southern Italy). By comparing the mean concentrations of CO and NO2 pollutants measured by MONICA devices and those simulated by SIRANE along the urban streets, the former appeared to exceed the simulated ones by a factor of 3 and 2 for CO and NO2, respectively. Furthermore, for each pollutant, this factor is higher within the street canyons than in open roads. However, the mobile and simulated mean concentration profiles largely adapt, although the simulated profiles appear smoother than the mobile ones. These results can be explained by the uncertainty in the estimation of vehicle emissions in SIRANE as well as the different temporal resolution of measurements of MONICA able to capture local high concentrations.
Terahertz Biosensor Based on Mode Coupling between Defect Mode and Optical Tamm State with Dirac Semimetal
Authors: Bao Yuwen , Ren Mengjiao , Ji Chengpeng , Dong Jun , Jiang Leyong , Dai Xiaoyu
Published: November 2022

Bulk Dirac semimetal (BDS) has emerged as a “3D graphene” material for the development of optical devices in the past few years. In this study, a BDS-based tunable highly sensitive terahertz (THz) biosensor is proposed by using a Dirac semimetal/Bragg reflector multilayer structure. The high sensitivity of the biosensor originates from the sharp Fano resonance peak caused by coupling the Optical Tamm State (OTS) mode and defect mode. Besides, the sensitivity of the proposed structure is sensitive to the Fermi energy of Dirac semimetal and the refractive index of the sensing medium. The maximum sensitivity of 1022°/RIU is obtained by selecting structural and material parameter appropriately, which has certain competitiveness compared to conventional surface plasmon resonance (SPR) sensors. From the standpoint of the fabrication facility and integration, we judged that the BDS-based layered structure has the potential application in biosensor field.
Processing of Chinese Base-Generated-Topic Sentences by L1-Korean Speakers: An Eye-Tracking Study
Authors: Song Kaiyan , Chang Hui , Wang Yuxia
Published: November 2022

According to the shallow structure hypothesis (SSH), adult L2 learners rely more on lexical-semantic and pragmatic information but less so on syntactic information in online language processing, ending up with shallower syntactic representation. To test the SSH, we conducted an eye-tracking experiment on L1-Korean L2-Chinese learners with native Chinese speakers as the baseline, investigating their processing of Chinese base-generated-topic sentences (BGT). The results show that both the intermediate and advanced Korean learners of Chinese are sensitive to and can make use of syntactic information, but only the advanced learners are sensitive to the semantic constraint when processing Chinese BGT sentences, providing evidence against the SSH.
Approaching the Gut and Nasal Microbiota in Parkinson’s Disease in the Era of the Seed Amplification Assays
Authors: Consonni Alessandra , Miglietti Martina , De Luca Chiara Maria Giulia , Cazzaniga Federico Angelo , Ciullini Arianna , Dellarole Ilaria Linda , Bufano Giuseppe , Di Fonzo Alessio , Giaccone Giorgio , Baggi Fulvio , Moda Fabio
Published: November 2022

Parkinson’s disease (PD) is a neurodegenerative disorder often associated with pre-motor symptoms involving both gastrointestinal and olfactory tissues. PD patients frequently suffer from hyposmia, hyposalivation, dysphagia and gastrointestinal dysfunctions. During the last few years it has been speculated that microbial agents could play a crucial role in PD. In particular, alterations of the microbiota composition (dysbiosis) might contribute to the formation of misfolded α-synuclein, which is believed to be the leading cause of PD. However, while several findings confirmed that there might be an important link between intestinal microbiota alterations and PD onset, little is known about the potential contribution of the nasal microbiota. Here, we describe the latest findings on this topic by considering that more than 80% of patients with PD develop remarkable olfactory deficits in their prodromal disease stage. Therefore, the nasal microbiota might contribute to PD, eventually boosting the gut microbiota in promoting disease onset. Finally, we present the applications of the seed amplification assays to the study of the gut and olfactory mucosa of PD patients, and how they could be exploited to investigate whether pathogenic bacteria present in the gut and the nose might promote α-synuclein misfolding and aggregation.
Kinematic Studies of the Go/No-Go Task as a Dynamic Sensorimotor Inhibition Task for Assessment of Motor and Executive Function in Stroke Patients: An Exploratory Study in a Neurotypical Sample
Authors: Lamp Gemma , Sola Molina Rosa Maria , Hugrass Laila , Beaton Russell , Crewther David , Crewther Sheila Gillard
Published: November 2022

Inhibition of reaching and grasping actions as an element of cognitive control and executive function is a vital component of sensorimotor behaviour that is often impaired in patients who have lost sensorimotor function following a stroke. To date, there are few kinematic studies detailing the fine spatial and temporal upper limb movements associated with the millisecond temporal trajectory of correct and incorrect responses to visually driven Go/No-Go reaching and grasping tasks. Therefore, we aimed to refine the behavioural measurement of correct and incorrect inhibitory motor responses in a Go/No-Go task for future quantification and personalized rehabilitation in older populations and those with acquired motor disorders, such as stroke. An exploratory study mapping the kinematic profiles of hand movements in neurotypical participants utilizing such a task was conducted using high-speed biological motion capture cameras, revealing both within and between subject differences in a sample of healthy participants. These kinematic profiles and differences are discussed in the context of better assessment of sensorimotor function impairment in stroke survivors.
Tinnitus and Traumatic Memory
Authors: Fagelson Marc
Published: November 2022

Events linked to post-traumatic stress disorder (PTSD) influence psychological and physical health through the generation, exacerbation, and maintenance of symptoms such as anxiety, hyperarousal, and avoidance. Depending upon circumstance, traumatic events may also contribute to the onset of tinnitus, post-traumatic headache, and memory problems. PTSD should be considered a psychological injury, andwhile tinnitus is a symptom, its onset and sound quality may be connected in memory to the injury, thereby evincingthe capacity to exacerbate the trauma’s effects. The myriad attributes, psychological and mechanistic, shared by tinnitus and PTSD offer tinnitus investigators the opportunity to draw from the rich and long-practiced strategies implemented for trauma counseling. Mechanisms and interventions understood through the lens of traumatic exposures may inform the clinical management of tinnitus disorder, and future studies may assess the effect of PTSD intervention on co-occurring conditions. This brief summary considered literature from both the hearing and trauma disciplines, with the goal of reviewing mechanisms shared between tinnitus and PTSD, as well as clinical reports supporting mutual reinforcement of both their symptoms and the effects of therapeutic approaches.
Exploring the Potential of Immersive Virtual Reality in the Treatment of Unilateral Spatial Neglect Due to Stroke: A Comprehensive Systematic Review
Authors: Martino Cinnera Alex , Bisirri Alessio , Chioccia Ilaria , Leone Enza , Ciancarelli Irene , Iosa Marco , Morone Giovanni , Verna Valeria
Published: November 2022

The present review aims to explore the use of Immersive Virtual Reality (IVR) in the treatment of visual perception in Unilateral Spatial Neglect (USN) after a stroke. PubMed, Scopus, Embase and Pedro databases were searched, from inception to 1 February 2022. All studies that investigated the effect of IVR on USN, such as outcome in the stroke population, have been included. The current comprehensive systematic review was performed following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) recommendations and was registered in the PROSPERO database [CRD42022311284]. Methodological quality was assessed through JBI critical appraisal tool. A total of 436 articles were identified through the database searches. A total of 10 articles, with a heterogeneous study design, which involved 77 patients with USN with low-to-moderate methodological quality, have been selected. Five out the included studies tested usability of IVR for assessed or treated visual perception deficits in USN, comparing the results with 134 healthy subjects. In the rest of studies that tested IVR such as treatment, three showed statistical positive results (p < 0.05) in visual perception outcome. To date, the literature has suggested the potential benefits in the use of IVR for the treatment of visual perception disorders in USN. Interestingly, IVR motivates patients during the rehabilitation process improving compliance and interest. The heterogeneity in the studies’ design and in IVR treatments indicate the need of future investigations in the consideration of potentiality and low-cost of this technology.
Autonomous Concrete Crack Semantic Segmentation Using Deep Fully Convolutional Encoder–Decoder Network in Concrete Structures Inspection
Authors: Pu Rundong , Ren Guoqian , Li Haijiang , Jiang Wei , Zhang Jisong , Qin Honglei
Published: November 2022

Structure health inspection is the way to ensure that structures stay in optimum condition. Traditional inspection work has many disadvantages in dealing with the large workload despite using remote image-capturing devices. This research focuses on image-based concrete crack pattern recognition utilizing a deep convolutional neural network (DCNN) and an encoder–decoder module for semantic segmentation and classification tasks, thereby lightening the inspectors’ workload. To achieve this, a series of contrast experiments have been implemented. The results show that the proposed deep-learning network has competitive semantic segmentation accuracy (91.62%) and over-performs compared with other crack detection studies. This proposed advanced DCNN is split into multiple modules, including atrous convolution (AS), atrous spatial pyramid pooling (ASPP), a modified encoder–decoder module, and depthwise separable convolution (DSC). The advancement is that those modules are well-selected for this task and modified based on their characteristics and functions, exploiting their superiority to achieve robust and accurate detection globally. This application improved the overall performance of detection and can be implemented in industrial practices.
Large-Scale 3D Printing for Construction Application by Means of Robotic Arm and Gantry 3D Printer: A Review
Authors: Puzatova Anastasia , Shakor Pshtiwan , Laghi Vittoria , Dmitrieva Maria
Published: November 2022

Additive manufacturing technologies are becoming more popular in various industries, including the construction industry. Currently, construction 3D printing is sufficiently well studied from an academic point of view, leading towards the transition from experimental to mass large-scale construction. Most questions arise about the applicability of construction 3D printers for printing entire buildings and structures. This paper provides an overview of the different types of construction 3D printing technologies currently in use, and their fundamental differences, as well as some significant data on the advantages of using these advanced technologies in construction. A description of the requirements for composite printing is also provided, with possible issues that may arise when switching from lab-scale construction printing to mass large-scale printing. All printers using additive manufacturing technologies for construction are divided into three types: robotic arm printers, portal-type printers, and gantry 3D printers. It is noted that gantry printers are more suitable for large-scale printing since some of their configurations have the ability to construct buildings that are practically unlimited in size. In addition, all printers are not capable of printing with concrete containing a coarse aggregate, which is a necessary requirement in terms of the strength and economic feasibility of 3D printing material for large-scale applications.
Dynamic Concentrated Solar Building Skin Design Based on Multiobjective Optimization
Authors: Shao Zebiao , Wang Bo , Xu Yao , Sun Liang , Ge Xichen , Cai Lvpei , Chang Cheng
Published: November 2022

Building skin can provide comprehensive functions of energy production, daylighting, and shading with an integrated transmissive solar-concentrating panel. In this study, Rhino Grasshopper parametric modeling, Ladybug tool performance simulation, and Octopus multiobjective optimization platforms are used to carry out experimental research. This study establishes the optimal relationship between the conflicting objectives of light environment creation and energy production efficiency of solar-concentrating skin by controlling three variables, namely the size of the solar-concentrating module, the rotation angle, and the number of modules, aiming to design the optimal solution and build a multiobjective optimization technology framework for the solar-concentrating skin of an office space. A comparison and analysis of the scenarios indicate a dynamic concentrating skin that can effectively reduce the daylight glare probability (DGP) by 70% and increase the useful daylight illuminance (UDI) by 10%, while achieving energy production. The correlation between the variables and the performance indices of the solar-concentrating skin was obtained as angle > width > length > amount, and the optimal design interval for each parameter variable. This study reveals the laws of how parameter changes affect individual indicators, which can provide ideas for the design of dynamic concentrating skins and building integration, methods for improving the balanced design of indoor light environments and building capacity, and a technical framework for multiobjective optimization processes.
Real-Time and Remote Construction Progress Monitoring with a Quadruped Robot Using Augmented Reality
Authors: Halder Srijeet , Afsari Kereshmeh , Serdakowski John , DeVito Stephen , Ensafi Mahnaz , Thabet Walid
Published: November 2022

Construction progress monitoring involves a set of inspection tasks with repetitive in-person observations on the site. The current manual inspection process in construction is time-consuming, inefficient and inconsistent mainly due to human limitations in the ability to persistently and accurately walkthrough the job site and observe the as-built status of which robots are considerably better. Enabling the process of visual inspection with a real-time and remote inspection capability using robots can provide more frequent and accessible construction progress data for inspectors to improve the quality of inspection and monitoring. Also, integrating remote inspection with an Augmented Reality (AR) platform can help the inspector to verify as-planned BIM data with the as-built status. This paper proposes a new approach to perform remote monitoring of the construction progress in real-time using a quadruped robot and an AR solution. The proposed computational framework in this study uses a cloud-based solution to integrate the quadruped robot’s control for remote navigation through the construction site with 360° live-stream video of the construction status, as well as a real-time AR solution to visualize and compare the as-built status with as-planned BIM geometry. The implementation of the proposed framework is discussed, and the developed framework is evaluated in two use cases through experimental investigations.
Critical Factors Influencing Cost Overrun in Construction Projects: A Fuzzy Synthetic Evaluation
Authors: Xie Wenwen , Deng Binchao , Yin Yilin , Lv Xindong , Deng Zhenhua
Published: November 2022

Construction industries have poor cost performance in terms of finishing projects within a budget. A fuzzy model for evaluating the critical factors of cost overrun for construction projects in China is developed by identifying, classifying and ranking cost overrun factors of the construction industries. Sixty-five cost overrun factors are identified and classified into four clusters (project macro, project management, project environment, and core stakeholders) through a detailed literature review process and a discussion with experts from the Chinese construction industry. A questionnaire survey was conducted for data collection to calculate an index of the project-influenced factors and clusters in the construction industry in China. With the help of the proposed model, it is possible to guide project managers and decision makers to make better informative decisions such as project macro, project management, project environment, and core stakeholders.
Science Mapping for Recent Research Regarding Urban Underground Infrastructure
Authors: Yin Xianfei , Wang Mingzhu
Published: November 2022

The presented research conducted a bibliometric analysis regarding academic publications, especially journal publications, in the area of urban underground infrastructure (UI) systems (which include sewer pipes, drinking water pipes, cables, tunnels, etc.). In total, 547 journal papers published from 2002 to July 2022 (around 20 years period) were retrieved from Scopus using the proposed data collection method. Bibliometric analysis was conducted to extract and map the hidden information from retrieved papers. As a result, networks regarding co-citation, co-authorship, and keywords co-occurrence were generated to visualise and analyse the knowledge domain, patterns, and relationships. The eight most investigated topics in the UI research are identified and discussed, which provides an overview of the research history and focuses. Further, five potential research directions are suggested for researchers in the UI research area. The main contribution of this research is on revealing the knowledge domain of UI research in a quantitative manner as well as identifying the possible research directions.
Allogeneic Hematopoietic Stem Cell Transplantation in Transformed Follicular Lymphoma (tFL): Results of a Retrospective Multicenter Study from GELTAMO/GETH-TC Spanish Groups
Authors: Rey-Búa Beatriz , Cabrero Mónica , Bento Leyre , Montoro Juan , Bastos-Oreiro Mariana , Parody Rocío , Yañez Lucrecia , Lopez-Godino Oriana , Zanabili Joud , Herrera Pilar , Gutierrez Gonzalo , Perez Ariadna , Piñana Jose L. , Novelli Silvana , Cortés María , Sureda Ana Maria , Caballero Dolores , García-Sancho Alejandro Martín
Published: November 2022

Background: Transformation of follicular lymphoma into an aggressive lymphoma (tFL) worsens the prognosis and the standard treatment is not completely defined. Allogeneic hematopoietic stem cell transplantation (alloSCT) could be a potentially curative option for these patients, but it has not been widely explored. Methods: We designed a retrospective multicenter study to analyze the efficacy and toxicity of alloSCT in tFL patients and potential prognostic factors of survival. Results: A total of 43 patients diagnosed with tFL who underwent alloSCT in 14 Spanish centers between January 2000 and January 2019 were included. Median age was 44 (31–67) years. After a median follow-up of 58 months, estimated 5-year overall survival (OS) and progression-free survival (PFS) were both 35%. Estimated 100-day and 1-year non-relapse mortality (NRM) were 20% and 34%, respectively. The type of conditioning regimen (3-year OS of 52% vs. 20%, respectively, for reduced-intensity vs. myeloablative conditioning) and development of chronic graft versus host disease (cGVHD) (3-year OS of 75% vs. 40%) were the only factors significantly associated with OS. The only variable with an independent association with OS was cGVHD (HR, 3.4; 95% CI, 1.2–9.6). Conclusions: Our results indicate that alloSCT continues to be a potentially curative option for patients with tFL.
Safety and Efficacy of Irreversible Electroporation in Locally Advanced Pancreatic Cancer: An Evaluation from a Surgeon’s Perspective
Authors: Shen Jian , Pan Penglin , Hu Xiaoli , Zhao Jun , Wu Heshui
Published: November 2022

Irreversible electroporation (IRE) has emerged as a promising treatment for patients with locally advanced pancreatic cancer (LAPC). Therefore, in this study, we evaluate the safety and efficacy of IRE against LAPC, as well as exploring its impact on anti-tumor immunity. A retrospective analysis was conducted in consecutive patients at a single institution. Eligible patients were assigned to IRE, palliative surgery (PS), or vascular resection (VR) groups, according to their respective treatments. The IRE group consisted of LAPC patients. One-to-one propensity score matching was performed, in order to compare the incidence of complications and median overall survival (mOS). Serum and intratumoral cytokines, as well as intratumoral immune cells, were analyzed in order to identify changes in immunity after IRE. A total of 210 patients were included. After matching, the rate of major complications (Clavien–Dindo III–V), intra-abdominal hemorrhage, and re-intervention in the IRE group were similar to those in the VR group (p > 0.05). The mOS of the IRE group (13.0 months) was shorter than that of the VR group (15.0 months), but longer than that of the PS group (8.0 months) (p < 0.05). Patients in the IRE group had elevated serum levels of immunogenic cytokines, including IL-2, IL-6, and TNF-α, which were related to anti-tumor immunity. The survival advantage in IRE-treated patients was attributed to tumor ablation and immune modulation effects. Overall, IRE can be considered a feasible treatment for patients with LAPC.
PSMA PET for the Evaluation of Liver Metastases in Castration-Resistant Prostate Cancer Patients: A Multicenter Retrospective Study
Authors: Mattoni Susanna , Farolfi Andrea , Formaggio Fabio , Bruno Gabriel , Caroli Paola , Cerci Juliano Julio , Eiber Matthias , Fendler Wolfgang Peter , Golfieri Rita , Herrmann Ken , Matteucci Federica , Mosconi Cristina , Paolani Giulia , Santoro Miriam , Strigari Lidia , Nanni Cristina , Castellucci Paolo , Fanti Stefano
Published: November 2022

Background: To evaluate the diagnostic performance of PSMA-PET compared to conventional imaging/liver biopsy in the detection of liver metastases in CRPC patients. Moreover, we evaluated a PSMA-PET/CT-based radiomic model able to identify liver metastases. Methods: Multicenter retrospective study enrolling patients with the following inclusion criteria: (a) proven CRPC patients, (b) PSMA-PET and conventional imaging/liver biopsy performed in a 6 months timeframe, (c) no therapy changes between PSMA-PET and conventional imaging/liver biopsy. PSMA-PET sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy for liver metastases were calculated. After the extraction of radiomic features, a prediction model for liver metastases identification was developed. Results: Sixty CRPC patients were enrolled. Within 6 months before or after PSMA-PET, conventional imaging and liver biopsy identified 24/60 (40%) patients with liver metastases. PSMA-PET sensitivity, specificity, PPV, NPV, and accuracy for liver metastases were 0.58, 0.92, 0.82, 0.77, and 0.78, respectively. Either number of liver metastases and the maximum lesion diameter were significantly associated with the presence of a positive PSMA-PET (p < 0.05). On multivariate regression analysis, the radiomic feature-based model combining sphericity, and the moment of inverse difference (Idm), had an AUC of 0.807 (95% CI:0.686-0.920). Conclusion: For liver metastases assessment, [68Ga]Ga-PSMA-11-PET demonstrated moderate sensitivity while high specificity, PPV, and inter-reader agreement compared to conventional imaging/liver biopsy in CRPC patients.
Surgical Outcomes of Video-Assisted versus Open Pneumonectomy for Lung Cancer: A Real-World Study
Authors: Luo Jizhuang , Ji Chunyu , Campisi Alessio , Chen Tangbing , Weder Walter , Fang Wentao
Published: November 2022

Background: The safety, feasibility and potential benefits of Video-assisted thoracoscopic surgery (VATS) pneumonectomy remain to be investigated. Methods: Patients receiving VATS or Open pneumonectomy during the study period were included to compare surgical outcomes. Propensity-score matched (PSM) analysis was performed to eliminate potential biases. Results: From 2013 to 2020, 583 consecutive patients receiving either VATS (105, 18%) or Open (478, 82%) pneumonectomy were included. Conversion from VATS to open was found in 20 patients (19.0%). The conversion patients had similar rates of major complications and perioperative mortality compared with the Open group. After PSM, 203 patients were included. No significant differences were observed in major complications and perioperative mortality between the two groups. For patients with stage pT2 tumors, the major complication rate in the VATS group was significantly lower than in the Open group (7.6% vs. 20.6%, p = 0.042). Compared with left pneumonectomy, the incidence of bronchopleural fistula (BPF) was significantly higher in right pneumonectomy for both VATS (0 vs. 16.7%, p = 0.005) and Open (0.7% vs. 6.5%, p = 0.002) approaches. Conclusions: Perioperative results of VATS pneumonectomy are non-inferior to those of the Open approach. Conversion to open surgery does not compromise perioperative outcomes. Patients with lower pT stage tumors who need pneumonectomy may benefit from VATS.
Gold Nanoparticles-Mediated Photothermal Therapy of Pancreas Using GATE: A New Simulation Platform
Authors: Asadi Somayeh , Bianchi Leonardo , De Landro Martina , Saccomandi Paola
Published: November 2022

This work presents the first investigation of gold nanorods (GNRs)-based photothermal therapy of the pancreas tumor using the Monte Carlo-based code implemented with Geant4 Application for Emission Tomography (GATE). The model of a human pancreas was obtained by segmenting an abdominal computed tomography (CT) scan, and its physical and chemical properties, were obtained from experimental and theoretical data. In GATE, GNRs-mediated hyperthermal therapy, simple heat diffusion as well as interstitial laser ablation were then modeled in the pancreas tumor by defining the optical parameters of this tissue when it is loaded with GNRs. Two different experimental setups on ex vivo pancreas tissue and GNRs-embedded water were devised to benchmark the developed Monte Carlo-based model for the hyperthermia in the pancreas alone and with GNRs, respectively. The influence of GNRs on heat distribution and temperature increase within the pancreas tumor was compared for two different power values (1.2 W and 2.1 W) when the tumor was exposed to 808 nm laser irradiation and with two different laser applicator diameters. Benchmark tests demonstrated the possibility of the accurate simulating of NPs-assisted thermal therapy and reproducing the experimental data with GATE software. Then, the output of the simulated GNR-mediated hyperthermia emphasized the importance of the precise evaluation of all of the parameters for optimizing the preplanning of cancer thermal therapy. Simulation results on temperature distribution in the pancreas tumor showed that the temperature enhancement caused by raising the power was increased with time in both the tumor with and without GNRs, but it was higher for the GNR-load tumor compared to tumor alone.
Discovery of Novel Lin28 Inhibitors to Suppress Cancer Cell Stemness
Authors: Radaeva Mariia , Ho Chia-Hao , Xie Ning , Zhang Sijie , Lee Joseph , Liu Liangliang , Lallous Nada , Cherkasov Artem , Dong Xuesen
Published: November 2022

Lin28 is a pluripotency factor that regulates cancer cell stem-like phenotypes to promote cancer development and therapy-resistant tumor progression. It acts through its cold shock domain and zinc knuckle domain (ZKD) to interact with the Let-7 pre-microRNA and block Let-7 biosynthesis. Chemical inhibition of Lin28 from interacting with Let-7 presents a therapeutic strategy for cancer therapy. Herein, we present the computer-aided development of small molecules by in silico screening 18 million compounds from the ZINC20 library, followed by the biological validation of 163 predicted compounds to confirm 15 new Lin28 inhibitors. We report three lead compounds, Ln7, Ln15, and Ln115, that target the ZKD of both Lin28A and Lin28B isoforms and block Lin28 from binding Let-7. They restore Let-7 expression and suppress tumor oncogenes such as SOX2 in cancer cells and show strong inhibitory effects on cancer cell stem-like phenotypes. However, minimal impacts of these compounds were observed on Lin28-negative cells, confirming the on-target effects of these compounds. We conclude from this study the discovery of several new Lin28 inhibitors as promising candidate compounds that warrant further drug development into potential anticancer therapies.
Non-Coding RNAs of Extracellular Vesicles: Key Players in Organ-Specific Metastasis and Clinical Implications
Authors: Jiang Qian , Tan Xiao-Ping , Zhang Cai-Hua , Li Zhi-Yuan , Li Du , Xu Yan , Liu Yu Xuan , Wang Lingzhi , Ma Zhaowu
Published: November 2022

Extracellular vesicles (EVs) are heterogeneous membrane-encapsulated vesicles released by most cells. They act as multifunctional regulators of intercellular communication by delivering bioactive molecules, including non-coding RNAs (ncRNAs). Metastasis is a major cause of cancer-related death. Most cancer cells disseminate and colonize a specific target organ via EVs, a process known as “organ-specific metastasis”. Mounting evidence has shown that EVs are enriched with ncRNAs, and various EV-ncRNAs derived from tumor cells influence organ-specific metastasis via different mechanisms. Due to the tissue-specific expression of EV-ncRNAs, they could be used as potential biomarkers and therapeutic targets for the treatment of tumor metastasis in various types of cancer. In this review, we have discussed the underlying mechanisms of EV-delivered ncRNAs in the most common organ-specific metastases of liver, bone, lung, brain, and lymph nodes. Moreover, we summarize the potential clinical applications of EV-ncRNAs in organ-specific metastasis to fill the gap between benches and bedsides.
Elevated Levels of Circulating Hsp70 and an Increased Prevalence of CD94+/CD69+ NK Cells Is Predictive for Advanced Stage Non-Small Cell Lung Cancer
Authors: Seier Sophie , Bashiri Dezfouli Ali , Lennartz Philipp , Pockley Alan Graham , Klein Henriette , Multhoff Gabriele
Published: November 2022

Non-small cell lung cancer (NSCLC) is the second most frequently diagnosed tumor worldwide. Despite the clinical progress which has been achieved by multimodal therapies, including radiochemotherapy, and immune checkpoint inhibitor blockade, the overall survival of patients with advanced-stage NSCLC remains poor, with less than 16 months. It is well established that many aggressive tumor entities, including NSCLC, overexpress the major stress-inducible heat shock protein 70 (Hsp70) in the cytosol, present it on the plasma membrane in a tumor-specific manner, and release Hsp70 into circulation. Although high Hsp70 levels are associated with tumor aggressiveness and therapy resistance, membrane-bound Hsp70 can serve as a tumor-specific antigen for Hsp70-primed natural killer (NK) cells, expressing the C-type lectin receptor CD94, which is part of the activator receptor complex CD94/NKG2C. Therefore, we investigated circulating Hsp70 levels and changes in the composition of peripheral blood lymphocyte subsets as potential biomarkers for the advanced Union for International Cancer Control (UICC) stages in NSCLC. As expected, circulating Hsp70 levels were significantly higher in NSCLC patients compared to the healthy controls, as well as in patients with advanced UICC stages compared to those in UICC stage I. Smoking status did not influence the circulating Hsp70 levels significantly. Concomitantly, the proportions of CD4+ T helper cells were lower compared to the healthy controls and stage I tumor patients, whereas that of CD8+ cytotoxic T cells was progressively higher. The prevalence of CD3−/CD56+, CD3−/NKp30, CD3−/NKp46+, and CD3−/NKG2D+ NK cells was higher in stage IV/IIIB of the disease than in stage IIIA but were not statistically different from that in healthy individuals. However, the proportion of NK cells expressing CD94 and the activation/exhaustion marker CD69 significantly increased in higher tumor stages compared with stage I and the healthy controls. We speculate that although elevated circulating Hsp70 levels might promote the prevalence of CD94+ NK cells in patients with advanced-stage NSCLC, the cytolytic activity of these NK cells also failed to control tumor growth due to insufficient support by pro-inflammatory cytokines from CD4+ T helper cells. This hypothesis is supported by a comparative multiplex cytokine analysis of the blood in lung cancer patients with a low proportion of CD4+ T cells, a high proportion of NK cells, and high Hsp70 levels versus patients with a high proportion of CD4+ T cells exhibiting lower IL-2, IL-4, IL-6, IFN-γ, granzyme B levels.
ACY1 Downregulation Enhances the Radiosensitivity of Cetuximab-Resistant Colorectal Cancer by Inactivating the Wnt/β-Catenin Signaling Pathway
Authors: Shan Wulin , Dai Chunyang , Zhang Huanhuan , Han Dan , Yi Qiyi , Xia Bairong
Published: November 2022

Treatment of cetuximab-resistant colorectal cancer (CRC) is a global healthcare problem. This study aimed to assess the effects of radiotherapy on cetuximab-resistant CRC and explore the underlying mechanism. We established a cetuximab-resistant HCT116 cell line (HCT116-R) by extracorporeal shock. Differentially expressed mRNAs were screened from cells treated with different radiation doses using second-generation high-throughput sequencing. Sequence data showed that ACY1 was significantly downregulated in HCT116-R cells after irradiation. Analysis of the GEO and TCGA datasets revealed that high ACY1 expression was associated with lymph node metastasis and a poor prognosis in CRC patients. In addition, immunohistochemistry results from CRC patients revealed that ACY1 protein expression was related to cetuximab resistance and lymph node metastasis. These findings suggested that ACY1 may function as an oncogene to promote CRC progression and regulate the radiosensitivity of cetuximab-resistant CRC. As expected, ACY1 silencing weakened the proliferation, migration, and invasion abilities of HCT116-R cells after radiotherapy. Mechanistically, TCGA data demonstrated that ACY1 expression was closely related to the Wnt/β-catenin pathway in CRC. We validated that radiotherapy first reduced β-catenin levels, followed by decreased expression of the metastasis-related protein E-cadherin. Silencing ACY1 dramatically enhanced these changes in β-catenin and E-cadherin after radiotherapy. In conclusion, ACY1 downregulation could enhance the radiosensitivity of cetuximab-resistant CRC by inactivating Wnt/β-catenin signaling, implying that ACY1 may serve as a radiotherapy target for cetuximab-resistant CRC.
COVID-19 and Lung Cancer Survival: An Updated Systematic Review and Meta-Analysis
Authors: Oldani Simone , Petrelli Fausto , Dognini Giuseppina , Borgonovo Karen , Parati Maria Chiara , Ghilardi Mara , Dottorini Lorenzo , Cabiddu Mary , Luciani Andrea
Published: November 2022

Introduction: The outbreak of COVID-19 poses an unprecedented challenge to global public health. Patients with cancer are at a higher risk during the SARS-CoV-2 pandemic. Patients with lung cancer and COVID-19 were compared to those without cancer and those with other malignancies for the main outcome of this study. The aim of this study was to evaluate the differences in susceptibility, disease severity, and mortality between lung cancer patients and the general population. Methods: Using PRISMA reporting guidelines, we conducted a systematic review and meta-analysis of the published literature. The Cochrane Library database, PubMed, EMBASE, and PubMed Central were comprehensively searched for published papers until 31 May 2022. A pooled risk ratio (OR) with 95% CI was presented as the result of this meta-analysis. Results: We included 29 studies involved 21,257 patients with lung cancer and SARS-CoV-2 infection. Analysis data showed that mortality in patients with lung cancer was significantly higher than that in patients without cancer (HR = 2.00 [95%CI 1.52, 2.63], p < 0.01) or with other malignancies (HR = 1.91 [95%CI 1.53, 2.39], p < 0.01). In addition, we also observed a higher risk of severe infection in terms of life-threatening or required ICU admission/mechanical ventilation for lung cancer patients (HR = 1.47 [95%CI 1.06, 2.03], p = 0.02) than for patients with no cancer or other malignancies. Regarding lung cancer as a risk factor for acquiring SARS-CoV-2 infection, we could not reach statistical significance (hazard ratio [HR] =2.73 [95%CI 0.84, 8.94], p = 0.1). Conclusion: Lung cancer represents an important comorbidity and modifies COVID-19 prognosis in terms of disease severity and mortality. More patients experience severe or even fatal events. Considering their inherent fragility, patients with lung cancer, and generally all oncological populations, should be treated more carefully during the COVID-19 pandemic.
Heterologous Lignan Production in Stirred-Tank Reactors—Metabolomics-Assisted Bioprocess Development for an In Vivo Enzyme Cascade
Authors: Steinmann Andrea , Finger Maurice , Nowacki Christian , Decembrino Davide , Hubmann Georg , Girhard Marco , Urlacher Vlada B. , Lütz Stephan
Published: November 2022

Towards establishing a prospective industrial microbial lignan production process, we set up and investigated the biotransformation of coniferyl alcohol to secoisolariciresinol with recombinant Escherichia coli in a stirred-tank reactor (STR). Initially, we tested different cofactor concentrations and antifoam additions in shake flasks. Next, we designed an STR batch bioprocess and tested aeration rates, pH regulation, and substrate-feeding strategies. Targeted metabolomics of phenylpropanoids and lignans assisted the bioprocess development by monitoring the lignan pathway activity. We found that the copper concentration and the substrate-feeding strategy had considerable impact on lignan production. Furthermore, time-resolved monitoring of pathway metabolites revealed two maximal intracellular lignan concentrations, the first shortly after induction of gene expression and the second after the cells entered the stationary growth phase. During STR cultivation, a maximal intracellular titer of 130.4 mg L−1 secoisolariciresinol was achieved, corresponding to a yield coefficient of 26.4 mg g−1 and a space–time yield of 2.6 mg L−1 h−1. We report for the first time the in-depth evaluation of microbially produced lignans in a well-controlled STR bioprocess. Monitoring of the lignan pathway activity showed that lignan accumulation is highly dynamic during the cultivation and points towards the need for a more efficient coniferyl alcohol dimerization system for optimal microbial production conditions.
Highly Active Ag-Cu Nanocrystal Catalyst-Coated Brewer’s Spent Grain Biochar for the Mineralization of Methyl Orange and Methylene Blue Dye Mixture
Authors: Boubkr Lahcen , Bhakta Arvind K. , Snoussi Youssef , Moreira Da Silva Cora , Michely Laurent , Jouini Mohamed , Ammar Souad , Chehimi Mohamed M.
Published: November 2022

The aim of the present work is to valorise the brewing industry’s waste, i.e., brewer’s spent grain (BSG), into functional biocarbon for environmental catalysis applications. In this context, cost-effective and environmentally friendly biochar support coated with in-situ-generated Ag-Cu nanocrystals, was developed via the wet impregnation of BSG biomass powder with copper (II) nitrate trihydrate and silver nitrate aqueous solution prior to pyrolysis at moderate temperature (500 °C). Small-size homogenously distributed Ag-Cu nanocrystals (≤80 nm) on the surface of the biochar (Biochar@Ag-Cu) were observed by field emission scanning electron microscope (FESEM) and transmission electron microscope (TEM). Elemental compositions were determined by X-ray photoelectron spectroscopy (XPS) and energy dispersive X-ray analysis (EDX). The crystalline nature of the nanoparticles was confirmed by X-ray powder diffraction (XRD). Information about the thermal stability of the materials and quality were obtained by thermogravimetric analysis (TGA) and Raman, respectively. The potentiality of the Biochar@Ag-Cu catalyst in the field of pollutant removal is demonstrated by taking methyl orange and methylene blue as model dyes. A kinetics study was performed and analyzed by UV–vis spectroscopy. Its highly active catalytic nature is proved by the complete mineralization of the methyl orange dye (100%) through oxidative degradation. The reusability of the catalyst has shown 96% removal efficiency after 3 cycles. The linear plot of −Ln (CA/C0) vs. time (R2 = 0.9892) reveals that the mineralization of the methyl orange dye follows pseudo-first-order kinetics (k = 0.603 × 10−2 min−1). A methyl orange + methylene blue dye mixture degradation study has revealed the faster kinetics of the present catalyst towards methylene blue degradation. The current study suggests that BSG Biochar@Ag-Cu can be a potential candidate in contribution towards SDG 6.
Methane-Assisted Iron Oxides Chemical Looping in a Solar Concentrator: A Real Case Study
Authors: Borghero Luca , Bressan Maurizio , Ferrero Domenico , Santarelli Massimo , Papurello Davide
Published: November 2022

Recent interest in hydrogen as an alternative fuel for lowering carbon emissions is funding the exploration of new ways to cleanly produce this molecule. Iron oxides can be used within a process of chemical looping. More specifically, they can lose oxygens at extremely high temperature in an inert atmosphere. An alumina receiver could not stand the extreme thermal stress, while steel (AISI 316 and Inconel Hastelloy c-276) lasted enough for the reaction to start, even if at the end of the process the receiver melted. Operating at a temperature above 1000 K helped the reaction switch from methane chemical looping combustion to chemical looping reforming, thus favouring H2 and CO yields. The gas flow outlet from the reactor reached a percentage up to 45% of H2 and 10% of CO. Carbon dioxide instead reached very low concentrations. While CO and CO2 reached a peak at the beginning of the experiment and then decreased, H2 was oscillating around a stable value. Unreacted methane was detected. The temperatures recorded in the reactor and the gas mixture obtained were used to validate a multiphysical model. The heat transfer and the chemistry of the experiment were simulated.