bluuebunny/crossref_metadata_2025 huggingface dataset:
Proteomic and metabolomic signatures of rectal tumor discriminate patients with different responses to preoperative radiotherapy
Authors: Wojakowska Anna , Marczak Lukasz , Zeman Marcin , Chekan Mykola , Zembala-Nożyńska Ewa , Polanski Krzysztof , Strugała Aleksander , Widlak Piotr , Pietrowska Monika
Published: February 2024

Background

Neoadjuvant radiotherapy (neo-RT) is widely used in locally advanced rectal cancer (LARC) as a component of radical treatment. Despite the advantages of neo-RT, which typically improves outcomes in LARC patients, the lack of reliable biomarkers that predict response and monitor the efficacy of therapy, can result in the application of unnecessary aggressive therapy affecting patients’ quality of life. Hence, the search for molecular biomarkers for assessing the radio responsiveness of this cancer represents a relevant issue.

Methods

Here, we combined proteomic and metabolomic approaches to identify molecular signatures, which could discriminate LARC tumors with good and poor responses to neo-RT.

Results

The integration of data on differentially accumulated proteins and metabolites made it possible to identify disrupted metabolic pathways and signaling processes connected with response to irradiation, including ketone bodies synthesis and degradation, purine metabolism, energy metabolism, degradation of fatty acid, amino acid metabolism, and focal adhesion. Moreover, we proposed multi-component panels of proteins and metabolites which could serve as a solid base to develop biomarkers for monitoring and predicting the efficacy of preoperative RT in rectal cancer patients.

Conclusion

We proved that an integrated multi-omic approach presents a valid look at the analysis of the global response to cancer treatment from the perspective of metabolomic reprogramming.
Clinical efficacy of plasmid encoding p62/SQSTM1 (Elenagen) in combination with gemcitabine in patients with platinum-resistant ovarian cancer: a randomized controlled trial
Authors: Krasny Sergei , Baranau Yauheni , Polyakov Sergey , Zharkova Ekaterina , Streltsova Olga , Filimonava Aliona , Siarheyeva Volha , Kazlouskaya Sviatlana , Khorau Anton , Gabai Vladimir , Shneider Alexander
Published: February 2024

Background

The purpose of this trial is to evaluate the safety and efficacy of ELENAGEN, a novel anticancer therapeutic DNA plasmid encoding p62/SQSTM1 protein, as an adjuvant to chemotherapy with gemcitabine (GEM) in patients with advanced platinum-resistant ovarian cancer.

Methods

This open-label prospective randomized study with two arms. GEM (1000 mg/m2) on days 1 and 8 every 3 weeks was administered in both arms: in the Chemo arm (n = 20), GEM was the only treatment, and in the ELENAGEN arm (n = 20), GEM was supplemented with ELENAGEN (2.5 mg i.m. weekly). The primary endpoint was progression-free survival (PFS), and the secondary endpoint was safety. Antitumor activity was assessed by RECIST 1.1, and criteria safety was assessed according to NCI CTCAE version 5.0.

Results

According to the cutoff data, the median follow-up was 13.8 months. There were no serious adverse events related to ELENAGEN treatment. The median PFS was 2.8 and 7.2 months in the Chemo and ELENAGEN arms, respectively (p Log-Rank = 0.03). Notably, at the time of cutoff, 9 patients (45%) in the ELENAGEN arm did not progress, with the longest PFS recorded thus far being 24 months. Subgroup analysis of patients in both arms demonstrated high efficacy of ELENAGEN in patients with worse prognostic factors: high pretreatment levels of CA125 and progression after platinum-free interval <3 months.

Conclusions

The addition of ELENAGEN to gemcitabine is effective in patients with platinum-resistant ovarian cancer, including those with a worse prognosis.

Clinical trial registration

https://www.clinicaltrials.gov/study/NCT05979298, identifier NCT05979298, 2023-08-07.
Clinical significance of bone marrow involvement by immunoglobulin gene rearrangement in de novo diffuse large B-cell lymphoma: a multicenter retrospective study
Authors: Kim Yu Ri , Shin Ho Jin , Yhim Ho-Young , Yang Deok-Hwan , Park Yong , Lee Ji Hyun , Lee Won-Sik , Do Young Rok , Mun Yeung-Chul , Kim Dae Sik , Kim Jin Seok
Published: February 2024

Background

Bone marrow (BM) involvement is an indicator of a poor prognosis in diffuse large B-cell lymphoma (DLBCL); however, few studies have evaluated the role of immunoglobulin gene rearrangement (IgR) in detecting BM involvement.

Methods

We evaluated the clinical characteristics and treatment outcomes of patients with DLBCL based on histological BM involvement or positive BM IgR using polymerase chain reaction or next-generation sequencing. We also investigated the role of consolidative upfront autologous hematopoietic stem cell transplantation (ASCT) in patients with DLBCL and BM involvement.

Results

Among 624 patients, 123 (19.7%) with histological BM involvement and 88 (17.5%) with positive IgR in histologically negative BM had more advanced disease characteristics. Overall (OS) and progression-free (PFS) survival was better for patients with negative BM histology and negative IgR than that in patients with histological BM involvement (P = 0.050 and P < 0.001, respectively) and positive IgR with negative BM histology (P = 0.001 and P = 0.005, respectively). Survival rates did not differ among 82 (13.1%) patients who were treated with upfront ASCT and had histological BM involvement or positive IgR with negative BM histology. The survival outcomes were worse for patients who were not treated with upfront ASCT and for those with histological BM involvement or positive IgR, than for those with negative BM histology and negative IgR.

Conclusion

Patients diagnosed with DLBCL and BM involvement based on histology or IgR had aggressive clinical features and poor survival. Upfront ASCT mitigated poor prognosis due to BM involvement.
Case Report: Severe vitamin D deficiency in a girl with inflammatory myopathy and myonecrosis
Authors: Kanouse Andrew , Nwosu Benjamin Udoka , Salemi Parissa
Published: February 2024

Inflammatory myopathies are a rare group of disorders that can cause significant disruption in the ability of an individual to adequately perform activities of daily living. In this case report, we present a case of a girl presenting with a substantial compromise of her ambulation with a muscle biopsy consistent with myonecrosis. She was subsequently diagnosed with an inflammatory myopathy and started on glucocorticoid and methotrexate therapy with minimal symptomatic improvement. Further in her clinical course, hypocalcemia and an undetectable 25-hydroxyvitamin D level were detected. Prompt institution of calcium and vitamin D supplementation significantly improved her myopathic condition. While there is evidence in the literature linking vitamin D deficiency with myopathy, there is a lack of data on the association between hypocalcemia and vitamin D deficiency with myonecrosis, which could represent comorbid states in myonecrosis. Therefore, vitamin D status should be established in all patients with myonecrosis, as vitamin D deficiency is easy to diagnose and treat, as exemplified in our patient’s case, which shows that such treatment could lead to significant clinical improvement.
Roles and inhibitors of FAK in cancer: current advances and future directions
Authors: Hu Hui-Hui , Wang Sai-Qi , Shang Hai-Li , Lv Hui-Fang , Chen Bei-Bei , Gao She-Gan , Chen Xiao-Bing
Published: February 2024

Focal adhesion kinase (FAK) is a non-receptor tyrosine kinase that exhibits high expression in various tumors and is associated with a poor prognosis. FAK activation promotes tumor growth, invasion, metastasis, and angiogenesis via both kinase-dependent and kinase-independent pathways. Moreover, FAK is crucial for sustaining the tumor microenvironment. The inhibition of FAK impedes tumorigenesis, metastasis, and drug resistance in cancer. Therefore, developing targeted inhibitors against FAK presents a promising therapeutic strategy. To date, numerous FAK inhibitors, including IN10018, defactinib, GSK2256098, conteltinib, and APG-2449, have been developed, which have demonstrated positive anti-tumor effects in preclinical studies and are undergoing clinical trials for several types of tumors. Moreover, many novel FAK inhibitors are currently in preclinical studies to advance targeted therapy for tumors with aberrantly activated FAK. The benefits of FAK degraders, especially in terms of their scaffold function, are increasingly evident, holding promising potential for future clinical exploration and breakthroughs. This review aims to clarify FAK’s role in cancer, offering a comprehensive overview of the current status and future prospects of FAK-targeted therapy and combination approaches. The goal is to provide valuable insights for advancing anti-cancer treatment strategies.
Modeling of flaxseed protein, oil content, linoleic acid, and lignan content prediction based on hyperspectral imaging
Authors: Zhu Dongyu , Han Junying , Liu Chengzhong , Zhang Jianping , Qi Yanni
Published: February 2024

Protein, oil content, linoleic acid, and lignan are several key indicators for evaluating the quality of flaxseed. In order to optimize the testing methods for flaxseed’s nutritional quality and enhance the efficiency of screening high-quality flax germplasm resources, we selected 30 flaxseed species widely cultivated in Northwest China as the subjects of our study. Firstly, we gathered hyperspectral information regarding the seeds, along with data on protein, oil content, linoleic acid, and lignan, and utilized the SPXY algorithm to classify the sample set. Subsequently, the spectral data underwent seven distinct preprocessing methods, revealing that the PLSR model exhibited superior performance after being processed with the SG smoothing method. Feature wavelength extraction was carried out using the Successive Projections Algorithm (SPA) and the Competitive Adaptive Reweighted Sampling (CARS). Finally, four quantitative analysis models, namely Partial Least Squares Regression (PLSR), Support Vector Regression (SVR), Multiple Linear Regression (MLR), and Principal Component Regression (PCR), were individually established. Experimental results demonstrated that among all the models for predicting protein content, the SG-CARS-MLR model predicted the best, with and of 0.9563 and 0.9336, with the corresponding Root Mean Square Error Correction (RMSEC) and Root Mean Square Error Prediction (RMSEP) of 0.4892 and 0.5616, respectively. In the optimal prediction models for oil content, linoleic acid and lignan, the Rp2 was 0.8565, 0.8028, 0.9343, and the RMSEP was 0.8682, 0.5404, 0.5384, respectively. The study results show that hyperspectral imaging technology has excellent potential for application in the detection of quality characteristics of flaxseed and provides a new option for the future non-destructive testing of the nutritional quality of flaxseed.
The effect of peat replacement in horticulture media by willow (Salix viminalis L.) biomass compost for cucumber transplant production
Authors: Adamczewska-Sowińska Katarzyna , Sowiński Józef , Jamroz Elżbieta , Bekier Jakub
Published: February 2024

This research evaluated the usefulness of horticultural substrates prepared on the basis of compost from chipped willow without additives and with the addition of nitrogen and decomposing mycelium of the cellulose-lignin fraction of wood in the cultivation of cucumber seedlings. The produced composts were mixed in different proportions: mixture 1 (W1) - the proportion of compost without additives and compost prepared with the addition of nitrogen and mycelium was in the ratio of 50:50, mixture 2 (W2) - the proportion of compost without additives and compost prepared with the addition of nitrogen and mycelium was in the ratio of 75:25. The starting mixtures were used to prepare horticultural substrates with different components (peat - P, vermicompost - V) and additives: basaltmeal - B, biochar from deciduous wood - C. The components were added in varying proportions. A total of 29 different substrates were subsequently tested in the study. Plant showed that the traits assessed varied to a greater extent under the effect of the test factors than at earlier growth stages. It was demonstrated that cucumber grown on substrates with 75% or 50% willow compost had a unit weight at the same statistical level as when grown on peat substrate (P). The plants with the highest unit weight (8.5- 10.4 g), belonged to the same homogeneous group and derived from sites W1P1B2, W2P1, W1P1B1, W2P2, W1P1C1, P, W1P1, W2B1, W2P2B2. High-quality cucumber transplant should characterise well develop, optimal height-to-stem thickness ratio, short hypocotyl, thick green leaves and cotyledons.
Measuring political legitimacy in two dimensions: internal and external measures
Authors: Harfst Philipp , Wiesner Claudia
Published: February 2024

Research on political legitimacy encompasses two distinct traditions, one institutionalist and another drawing on political culture accounts of legitimacy. Recent contributions argue in favor of an integration of these two vantage points in the study of political legitimacy. Following these lines of reasoning, we investigate empirically whether a two-dimensional approach in political legitimacy research integrating the institutionalist and political culture perspectives can contribute to establish a more comprehensive and nuanced view on political regimes and their legitimacy. We conceptualize political legitimacy in combining an external normative with an internal empirical perspective, collect data for both and compare the relationship between these two dimensions of political legitimacy in an internationally comparative framework. Using data from Integrated Values Surveys and Varieties of Democracy we find that both dimensions are closely linked in general, discover important deviations from this pattern in the case of citizens' performance evaluations, pinpoint a particular group of hybrid cases that either lost internal or external legitimacy while collecting positive evaluations on the other dimension, and discuss the consequences of these findings for regime stability and future research on political legitimacy.
Motor strength as a feature of concepts and visual representations
Authors: Khatin-Zadeh Omid , Hu Jiehui , Farsani Danyal
Published: February 2024

In this article, we define motor strength as the extent to which a concept is associated with body movements and the motor system that guides body movements. We extend this notion to one of the features of visual representations of some concepts and discuss the role of the motor system in understanding concepts and visual representations that have a significant degree of motor strength. It is suggested that when a concept is understood in its literal sense, the employment of the motor system and gestures in processing that concept depends on its degree of motor strength. If a concept is understood in its metaphorical sense, the employment of the motor system and gestures is dependent on the degree of motor strength of the base of the metaphor through which that concept is understood. The degree of motor strength of a concept relies on its motor affordances and its associations with people’s past experiences. Because the motor system plays an essential role in the grounding of many abstract concepts in the physical environment, the notion of motor strength can help psychologists acquire a clearer understanding of how concepts with varying degrees of motor strength are grounded in the physical environment.
The role of contextual factors in avenues to recover from gambling disorder: a scoping review
Authors: Johannessen Dagny Adriaenssen , Overå Stian , Arnevik Espen Ajo
Published: February 2024

Introduction

Recovery from complex conditions such as gambling disorders (GD) often entail considerable change and require a range of adaptable interventions in the health care system. Outcomes from such avenues to change are influenced by multifarious contextual factors, which are less frequently considered in treatment outcome studies. Accordingly, this scoping review aims to map the level of evidence and explore how contextual factors influence the provision and outcomes of GD interventions.

Methods

A systematic search in selected health and social science research databases yielded a total of 2.464 unique references. The results were screened in three selection steps—titles (n = 2.464), abstracts (n = 284) and full-text (n = 104). The scoping approach was applied to provide a narrative account of the final included references (n = 34).

Results and discussion

Findings suggest that the research on GD treatment is in the early stages of development. Additionally, studies on GD interventions are characterized by cultural biases (Region and ethnicity and Gender perspectives), while three key elements are described as successful avenues to recover from GD (Competence, Perception and Utilization). In line with these findings, proposals for future research and treatment designs are made.
School refusal: mapping the literature by bibliometric analysis
Authors: Ulaş Sümeyye , Gonzálvez Carolina , Seçer İsmail
Published: February 2024

School refusal is considered a risk factor for academic, social, and personal situations, such as school dropouts. Studies have been carried out on school refusal for almost 50 years. However, general research trends have not been mapped yet. This study summarizes the bibliometric analysis of scientific collaborations and prevalence across locations by country and institution, leading researchers, journals, and trends (keywords) in school refusal research. The United States, Japan, Spain, and England are the countries that stand out in terms of school refusal. It can be said that the Journal of American Academy of Child and Adolescent Psychiatry, Cognitive and Behavioral Practice, and Frontiers in Psychology are important journals that publish on school refusal. Researchers named Christopher A. Kearney, Carolina Gonzálvez, Jose Manuel Garcia-Fernandez, David A. Heyne, and Brigit M. Van Widenfelt have been found to have more intensive studies and collaborations on school refusal. The authors keywords common use for school refusal; are truancy, school absenteeism, adolescence, school attendance, school phobia, autism spectrum disorder, and bullying. The findings show that school refusal is a current research area, and scientific collaborations continue to be established. The findings reveal all the details of the school refusal research.
Regulation of miRNA expression in the prefrontal cortex by fecal microbiota transplantation in anxiety-like mice
Authors: Chen Simin , Li Mengjia , Tong Changqing , Wang Yanan , He Jiahui , Shao Qi , Liu Yan , Wu Ying , Song Yuehan
Published: February 2024

Background

The gut-brain axis and gut microbiota have emerged as key players in emotional disorders. Recent studies suggest that alterations in gut microbiota may impact psychiatric symptoms through brain miRNA along the gut-brain axis. However, direct evidence linking gut microbiota to the pathophysiology of generalized anxiety disorder (GAD) via brain miRNA is limited. In this study, we explored the effects of fecal microbiota transplantation (FMT) from GAD donors on gut microbiota and prefrontal cortex miRNA in recipient mice, aiming to understand the relationship between these two factors.

Methods

Anxiety scores and gut microbiota composition were assessed in GAD patients, and their fecal samples were utilized for FMT in C57BL/6J mice. Anxiety-like behavior in mice was evaluated using open field and elevated plus maze tests. High-throughput sequencing of gut microbiota 16S rRNA and prefrontal cortex miRNA was performed.

Results

The fecal microbiota of GAD patients exhibited a distinct microbial structure compared to the healthy group, characterized by a significant decrease in Verrucomicrobia and Akkermansia, and a significant increase in Actinobacteria and Bacteroides. Subsequent FMT from GAD patients to mice induced anxiety-like behavior in recipients. Detailed analysis of gut microbiota composition revealed lower abundances of Verrucomicrobia, Akkermansia, Bifidobacterium, and Butyricimonas, and higher abundances of Deferribacteres, Allobaculum, Bacteroides, and Clostridium in mice that received FMT from GAD patients. MiRNA analysis identified five key miRNAs affecting GAD pathogenesis, including mmu-miR-10a-5p, mmu-miR-1224-5p, mmu-miR-218-5p, mmu-miR-10b-5p, and mmu-miR-488-3p. Notably, mmu-miR-488-3p showed a strong negative correlation with Verrucomicrobia and Akkermansia.

Conclusion

This study demonstrates that anxiety-like behavior induced by human FMT can be transmitted through gut microbiota and is associated with miRNA expression in the prefrontal cortex. It is inferred that the reduction of Akkermansia caused by FMT from GAD patients leads to the upregulation of mmu-miR-488-3p expression, resulting in the downregulation of its downstream target gene Creb1 and interference with its related signaling pathway. These findings highlight the gut microbiota’s crucial role in the GAD pathophysiology.
The association between polychlorinated dibenzo-p-dioxin exposure and cancer mortality in the general population: a cohort study
Authors: Zheng Lei , Zhang Xianli , Gao Zhe , Zhong Chunyu , Qiu Dezhi , Yan Qing
Published: February 2024

Introduction

Earlier research has indicated that being exposed to polychlorinated dibenzo-p-dioxins (PCDDs) in the workplace can heighten the likelihood of cancer-related deaths. Nevertheless, there is limited information available regarding the connection between PCDD exposure and the risk of cancer mortality in the general population (i.e., individuals not exposed to these substances through their occupation).

Methods

The National Health and Nutrition Examination Survey (NHANES) detected PCDDs in the general population, and the death data were recently updated as of December 31, 2019. We conducted Cox regression analysis and controlled for covariates including age, gender, ethnicity, educational attainment, physical activity, alcohol intake, NHANES survey period, BMI category, cotinine concentration, and household earnings.

Results

After accounting for confounding factors, the findings indicated that for each incremental rise of 1 log unit in 1,2,3,4,6,7,8,9-octachlorodibenzo-p-dioxin, there was a 76% rise in the likelihood of death from any cause, with a p value of 0.003. An increase of 1 log unit in the concentration of 1,2,3,4,6,7,8-heptachlorodibenzofuran could potentially lead to a 90% higher risk of cancer mortality, as indicated by a p value of 0.034 and a 95% confidence interval of 0.05–2.43. As the concentrations of 1,2,3,4,6,7,8-heptachlorodibenzofuran increased, the dose–response curve indicated a proportional rise in the risk of cancer mortality, accompanied by a linear p value of 0.044. The sensitivity analysis demonstrated that our findings were resilient.

Discussion

In the general population, an elevated risk of cancer mortality was observed in PCDDs due to the presence of 1,2,3,4,6,7,8-heptachlorodibenzofuran. Mechanistic research is required to further confirm it.
Topic modeling and social network analysis approach to explore diabetes discourse on Twitter in India
Authors: Ramamoorthy Thilagavathi , Kulothungan Vaitheeswaran , Mappillairaju Bagavandas
Published: February 2024

Introduction

The utilization of social media presents a promising avenue for the prevention and management of diabetes. To effectively cater to the diabetes-related knowledge, support, and intervention needs of the community, it is imperative to attain a deeper understanding of the extent and content of discussions pertaining to this health issue. This study aims to assess and compare various topic modeling techniques to determine the most effective model for identifying the core themes in diabetes-related tweets, the sources responsible for disseminating this information, the reach of these themes, and the influential individuals within the Twitter community in India.

Methods

Twitter messages from India, dated between 7 November 2022 and 28 February 2023, were collected using the Twitter API. The unsupervised machine learning topic models, namely, Latent Dirichlet Allocation (LDA), non-negative matrix factorization (NMF), BERTopic, and Top2Vec, were compared, and the best-performing model was used to identify common diabetes-related topics. Influential users were identified through social network analysis.

Results

The NMF model outperformed the LDA model, whereas BERTopic performed better than Top2Vec. Diabetes-related conversations revolved around eight topics, namely, promotion, management, drug and personal story, consequences, risk factors and research, raising awareness and providing support, diet, and opinion and lifestyle changes. The influential nodes identified were mainly health professionals and healthcare organizations.

Discussion

The study identified important topics of discussion along with health professionals and healthcare organizations involved in sharing diabetes-related information with the public. Collaborations among influential healthcare organizations, health professionals, and the government can foster awareness and prevent noncommunicable diseases.
NUA 45, a dry bean cultivar, builds a subtle brand in Zimbabwe against all odds
Authors: Maereka Enock Kuziwa , Mutari Bruce , Kunaka Fungai , Nchanji Eileen , Tsekenedza Shylet , Muthoni-Andriatsitohaina Rachel , Chimboza Denford
Published: February 2024

Global business today is driven by brands, each with a unique story, but rarely of a product that is a public good. Building a brand requires careful planning, creativity, and an understanding of the target market. Borrowing from various brand concepts and frameworks, this paper explores how a government-bred common bean cultivar., NUA 45, defied the odds to create a new identity and association in Zimbabwe. Firstly, the cultivar overcame the general lack of promotion investment in government-bred (public) common bean cultivars. Secondly, being a red mottle-colored cultivar in a red-speckled (sugar type) bean-predominated country, NUA 45 had to break the preference typecasts. Thirdly, NUA 45 was among the first generation of biofortified bean cultivars enriched with iron (Fe) and zinc (Zn), some of the most sought-after micronutrients in a world ridden with hidden hunger. Overcoming these market barriers required a systematic approach to awareness creation, collaboration, and commercialization, avoiding the tragedy of the commons, and instead, achieving a win-win situation for all stakeholders. This entailed understanding the needs and preferences of different value chain actors, developing messages, and engaging local farmers, retailers, aggregators, and private and public sector partners to promote NUA 45. Lastly, developing compelling stories and leveraging digital channels to reach new customers that sought personal experiences, moved the cultivar name from a mere label to a spontaneously endorsed lifestyle and a brand that ‘spread like wildfire’ across the country and the Southern Africa Development Community (SADC) region. The NUA 45 brand broke new ground for other biofortified bean cultivars and non-sugar bean cultivars. Following its release in seven other countries, NUA 45 pioneered listing on the SADC regional variety catalog.
Case Report: Acute common peroneal nerve injury after posterior lumbar decompression surgery
Authors: Wang Peng Wei , Chung Ming Hsuan , Hueng Dueng Yuan , Hsia Chung Ching
Published: February 2024

Spine surgery is a prevalently performed procedure. Some authors have proposed an age-related surge in surgical and general complications. During spine surgery, patients are placed in positions that are not physiologic, would not be tolerated for prolonged periods by the patient in the awake state, and may lead to complications. Understanding these uncommon complications and their etiology is pivotal to prevention and necessary. The patient is a 76-year-old woman referred to the outpatient department of neurosurgery in February 2022 by her physiatrist with a chief complaint of chronic low back pain and numbness over the left leg. Lumbar spine magnetic resonance imaging revealed degenerative disc disease and posterior disc bulging at the levels of L2/3∼L5/S1 with compression of the thecal sac. After receiving anti-inflammatory medication, nerve block and caudal block, her symptoms persisted. She was referred to a neurosurgeon for surgical intervention. We diagnosed spinal stenosis with left L3 and L4 radiculopathy, and elective decompression surgery was scheduled a few days later. We performed discectomies at L2/3 and L3/4 and left unilateral laminectomy at L2 and L3 for bilateral decompression. Following an uneventful surgery, the patient was extubated, and her left leg pain improved, but pain over the right outer calf with drop foot developed. A second lumbar MRI the next day revealed no evidence of recurrent disc herniation or epidural hematoma. Then, she received nerve conduction velocity and needle electromyogram on postoperative day 2, and the studies indicated right common peroneal nerve entrapment neuropathy. After medication with steroids and foot splint use, right leg pain improved. However, weak dorsiflexion of the right ankle persisted. We referred this patient to a physiatrist and OPD for follow-up after discharge. Perioperative peripheral nerve injury (PPNI) is most commonly caused by peripheral nerve ischemia due to abnormal nerve lengthening or pressure and can be exacerbated by systemic hypotension. Any diseases affecting microvasculature and anatomical differences may contribute to nerve injury or render patients more susceptible to nerve injury. Prevention, early detection and intervention are paramount to reducing PPNI and associated adverse outcomes. The use of intraoperative neuromonitoring theoretically allows the surgical team to detect and intervene in impending PPNI during surgery.
Safety evaluation of a buffer used in the lyophilization of canine platelets: type I hypersensitivity reaction in dogs caused by bovine serum albumin
Authors: Choi Hee-Jae , Kim Mu-Young , Han Hyun-Jung
Published: February 2024

Introduction

The present study was designed to evaluate the safety of substances generally used in the preparation of lyophilized platelet products (LPPs) because the possibility of an immune response to bovine serum albumin (BSA) was considered high when using previously described technology.

Methods

An intradermal skin test, followed by a drug provocation test, was conducted to observe adverse events and identify the substances responsible for an immune response. Five male beagles (2 years old) weighing 12–14 kg were used. The dogs were clinically healthy and had no history of medication use. An intradermal skin test was conducted with each substance [i.e., 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid, sodium chloride, potassium chloride, sodium bicarbonate, theophylline, trehalose, and BSA] used in the conventional freeze-dry method.

Results

In the intradermal skin test, three dogs tested positive at the BSA injection site and showed clinical signs after the intradermal injection, including nausea and vomiting. For the drug provocation test, all dogs received two intravenous injections of an LPP buffer solution. The initial injection was devoid of BSA, whereas the subsequent injection contained BSA. The three dogs that had reacted to BSA in the intradermal skin test exhibited adverse events such as lethargy, vomiting, and nausea immediately after intravenous injection of the LPP buffer containing BSA. All dogs recovered uneventfully after symptomatic treatment in both tests.

Discussion

The high incidence and severity of type I hypersensitivity reactions observed in this study suggested that BSA is unsuitable as a component of canine LPP.
Actuators for Improving Robotic Arm Safety While Maintaining Performance: A Comparison Study
Authors: Xu Jiawei , Bone Gary M.
Published: February 2024

Since robotic arms operating close to people are becoming increasingly common, there is a need to better understand how they can be made safe when unintended contact occurs, while still providing the required performance. Several actuators and methods for improving robot safety are studied and compared in this paper. A robotic arm moving its end effector horizontally and colliding with a person’s head is simulated. The use of a conventional electric actuator (CEA), series elastic actuator (SEA), pneumatic actuator (PA) and hybrid pneumatic electric actuator (HPEA) with model-based controllers are studied. The addition of a compliant covering to the arm and the use of collision detection and reaction strategies are also studied. The simulations include sensor noise and modeling error to improve their realism. A systematic method for tuning the controllers fairly is proposed. The motion control performance and safety of the robot are quantified using root mean square error (RMSE) between the desired and actual joint angle trajectories and maximum impact force (MIF), respectively. The results show that the RMSE values are similar when the CEA, SEA, and HPEA drive the robot’s first joint. Regarding safety, using the PA or HPEA with a compliant covering can reduce the MIF below the safety limit established by the International Organization for Standardization (ISO). To satisfy this ISO safety limit with the CEA and SEA, a collision detection and reaction strategy must be used in addition to the compliant covering. The influences of the compliant covering’s stiffness and the detection delay are also studied.
Modeling of Precise Tension with Passive Dancers for Automated Fiber Placement
Authors: Li Yan , Che Zhe , Zheng Chenggan , Li Zhi , Wang Han , Cheng Liang , Jiang Junxia
Published: February 2024

This paper modeled the tension fluctuation during automated fiber placement (AFP), which depicted the tension variations under different operating conditions. The stability and validity of the model were demonstrated using Bode plots and experiments, respectively. According to the model, the tension fluctuations of AFP at different stages were obtained. Additionally, the passive dancer parameters with the better system performance were determined using the evaluation methodology presented in this paper. Moreover, it was discovered that the damping coefficient affects the tension variation more significantly than the elasticity coefficient. Finally, the placement experiments showed that the determined passive dancer parameters improved the laying quality significantly.
Effect of Ultraviolet B Radiation on the Biosynthesis of Carotenoids in Foxtail Millet Grains
Authors: Huo Yiqiong , Pang Xiangdong , Feng Huixia , Hao Ruili , Zhang Ruijie , Wang Yushen , Wang Haigang , Zhang Bin , Han Yuanhuai
Published: February 2024

Foxtail millet is a vital nutritional cereal. The de-husked grain is usually yellow and mainly contains carotenoids, which directly reflects the millet quality. In this study, the impact of ultraviolet-B(UV-B) on millet color and carotenoid content was determined using two foxtail millet varieties, HuaJinZao (HJZ) and Qinhuang 2 (QH). The b* value at different stages of grain development and the content of carotenoids (primarily lutein and zeaxanthin) in foxtail millet grains decreased when the plants were exposed to low UV-B intensity. A total of 3113 and 96 differentially expressed genes were identified in HJZ and QH, respectively, and were found to be associated with the metabolism of tryptophan, starch, and sucrose as well as the biosynthesis of amino acids, which was relatively consistent with the functional annotation of differential metabolites. Furthermore, we evaluated the changes in the expression of seven and eight genes associated with carotenoid and starch metabolism, respectively, in the kernels of foxtail millet exposed to UV-B and found that appropriate UV-B intensity could promote the expression levels of genes involved in carotenoid synthesis and repress the expression of genes involved in carotenoid degradation. This study lays a theoretical foundation for cultivating new foxtail millet varieties with high carotenoid content.
Does Digital Agricultural Technology Extension Service Enhance Sustainable Food Production? Evidence from Maize Farmers in China
Authors: Lun Runqi , Liu Wei , Li Guojing , Luo Qiyou
Published: February 2024

This research aims to investigate the average and heterogeneous impacts of digital agricultural technology extension service use on eco-efficiency among 1302 maize-producing farmers from a major maize-producing area in Northeast China in 2022. The slack-based measure model with undesirable outputs is applied to calculate the eco-efficiency of maize production. To obtain an unbiased estimation of the average effect, the self-selection problem generated by observable and unobservable factors is solved by the endogenous switching regression model. Quantile regression is utilized to analyze the heterogeneous effect. Notably, the mediated effects model is utilized to examine the potential mechanism between them. Our findings indicate that digital agricultural technology extension service use can increase maize production’s eco-efficiency. Digital agricultural technology extension service users would have reduced the eco-efficiency of the service by 0.148 (21.11%) if they had not used it. Digital agricultural technology extension service nonusers would have improved the eco-efficiency of the service by 0.214 (35.20%) if they had used it. The robustness check reconfirms the results. Moreover, digital agricultural technology extension service use is more helpful for maize farmers who have lower eco-efficiency than those who have higher eco-efficiency. Digital agricultural technology extension service use can improve the eco-efficiency of maize production through the application of organic fertilizers, green pesticides, and biodegradable agricultural films. There are policy implications of these findings: there is an argument for using the publicity of the digital agricultural technology extension service to encourage farmers to use sustainable inputs; additionally, it might be worthwhile to implement a categorized promotion strategy based on the different real-world situations.
Sorghum Densification with Changes in Plant Spacing Arrangement: Productivity and Qualitative Characteristics of Silage Material
Authors: Herrera Dayenne M. , Peixoto Wender M. , Abreu Joadil G. de , Reis Rafael H. P. dos , Cabral Carlos E. A. , Barros Livia V. de , Klein Vanderley A. C. , Passos Edmilson F. dos
Published: February 2024

The aim of this study was to evaluate the agronomic performance of sorghum grown in different combinations of row spacing and plant density, as well as possible interferences on silage quality. No other study dedicated to identifying the interference of plant spatial arrangement on the cultivation of silage material has been developed in the productive context of the Amazon Biome, making it necessary to understand the behavior of the studied factors. The treatments were arranged in a split-plot scheme: the plots corresponded to three row spacings (0.45 m, 0.60 m, and 0.75 m) and subplots at four densities (105,000, 120,000, 135,000, and 150,000 plants ha−1). The agronomic and productivity characteristics of sorghum and the fermentative and bromatological characteristics of forage and silage were evaluated. The sorghum plants showed an increase in plant height and green and dry mass yield when using higher densities (p < 0.05). For the culm diameter variable, an isolated effect of the factors was observed, with reduced diameter when grown closer to inter-row spacing or using higher plant densities. No effect of the factors was found (p > 0.05) for morphological plant components. In silage, wider spacing promoted higher dry matter content. Regarding crude protein in the silage, higher percentages were obtained at closer spacing and higher plant density. The sorghum growing in dense conditions is indicated, given the positive performance in productivity and bromatological composition.
Clinicopathologic Characterization of Prostatic Cancer in Dogs
Authors: Vasilatis Demitria M. , Ghosh Paramita M.
Published: February 2024

Clinicopathologic data in dogs with prostate cancer (PCa) may aid in the differentiation between tumor types and subsequent treatment decisions; however, these data are often unreported. Demographic, clinicopathologic, cytologic, histologic and survival data from dogs with primary prostatic adenocarcinoma (PRAD) (n = 56) and primary prostatic transitional cell carcinoma (P-TCC) (n = 74) were acquired from a tertiary veterinary teaching hospital from 1992 to 2022. Red blood cell distribution width (RDW) to albumin ratio (RAR) was evaluated for diagnostic utility in differentiating between PRAD and P-TCC. Sections from PRAD tumors (n = 50) were stained for androgen receptor (AR) expression, and laboratory data were compared between AR positive (AR+) and AR negative (AR−) groups. RDW was increased in PRAD, while albumin was decreased (p < 0.05). P-TCC was associated with Melamed-Wolinska bodies (MWB) and necrosis on cytology (p < 0.05). RAR had acceptable diagnostic utility in the differentiation of PCa tumors (AUC = 0.7; p < 0.05). Survival rates and metastases were equivocal. AR+ and AR− PRAD tumors did not differ in clinicopathologic data or survival (p > 0.05). In conclusion, hypoalbuminemia was significantly associated with PRAD and decreased survival, while MWB and necrosis were significantly associated with P-TCC on cytology. These clinicopathologic data may help clinicians differentiate between these tumors ante mortem to guide appropriate treatment and intervention.
Metabolic and Endocrine Insights in Donkeys
Authors: Mendoza Francisco J. , Toribio Ramiro E. , Perez-Ecija Alejandro
Published: February 2024

Donkey medicine is gaining attention due to their increased use as companion animals, in shows, asinotherapy, etc. The increasing demand and unique aspects call for specialized care, requiring new information (physiology, infectious disorders, pharmacology, etc.). Since obesity is common in this species, hyperlipemia, metabolic syndrome and insulin dysregulation (ID) are common disorders in donkeys, in some cases with high mortality, either directly (multiorgan dysfunction) or indirectly due to poor quality of life (chronic laminitis). Donkeys have long-life expectancy and are often afflicted with pituitary pars intermedia dysfunction (PPID), a neurodegenerative and endocrine disease. Hyperlipemia is diagnosed based on high plasma triglyceride concentration in association with clinical findings and laboratory abnormalities from affected tissues (liver, kidney and pancreas). The measurement of resting serum insulin and plasma ACTH concentrations is the first step in ID and PPID diagnosis. In donkeys with clinical signs of ID (obesity or recurrent laminitis) or PPID (hypertrichosis, regional adiposity, laminitis and weight loss), where these hormones are in the normal or non-diagnostic range (donkey-specific cut-off values and reference ranges need to be established), dynamic tests are recommended (oral sugar test or thyrotropin-releasing hormone, respectively). Equine treatment protocols apply to donkeys, although pharmacological studies for most drugs, except pergolide, are lacking.
Assessment of Full-Fat Tenebrio molitor as Feed Ingredient for Solea senegalensis: Effects on Growth Performance and Lipid Profile
Authors: Hachero-Cruzado Ismael , Betancor Mónica B. , Coronel-Dominguez Antonio Jesús , Manchado Manuel , Alarcón-López Francisco Javier
Published: February 2024

Tenebrio molitor (TM) is considered as one of the most promising protein sources for replacing fish meal in aquafeeds, among other things because it is rich in protein, a good source of micronutrients and has a low carbon footprint and land use. However, the main drawback of TM is its fatty acid profile, in particular its low content of n-3 PUFA. This study evaluates the effects of partially replacing plant or marine-derived with full-fat TM meal at two different levels on growth performance and lipid profiles of Senegalese sole (Solea senegalensis). For this purpose, a control diet (CTRL) and four experimental isoproteic (53%) and isolipidic (16%) diets were formulated containing 5 and 10% TM meal replacing mostly fish meal (FM5 and FM10), or 10 and 15% TM meal replacing mostly plant meal (PP10 and PP15). Fish (215 g) were fed at 1% of their body weight for 98 days. The final body weight of fish fed the experimental diets containing TM meal was not different from that of fish fed the CTRL diet (289 g). However, the inclusion of TM meal resulted in a gradual improvement in growth rate and feed efficiency in both cases (replacement of fish or plant meals), and significant differences in specific growth rate (SGR) were observed between fish fed the CTRL diet (SGR = 0.30% day−1) and those fed diets with the highest TM meal content (PP15; SGR = 0.35% day−1). The experimental groups did not show any differences in the protein content of the muscle (19.6% w/w). However, significant differences were observed in the total lipid content of the muscle, with the FM10, PP10, and PP15 groups having the lowest muscle lipid contents (2.2% ww). These fish also showed the lowest neutral lipid content in muscle (6.6% dw), but no differences were observed in the total phospholipid content (2.6% dw). Regarding the fatty acid profile, fish fed FM10, PP10 and PP15 had lower levels of linoleic acid (18:2n-6) and higher levels of oleic acid (18:1n-9) in liver and muscle compared to fish fed CTRL. However, no differences were found between fish fed CTRL and TM-based diets for docosahexaenoic acid (22:6n-3) and total n-3 PUFA in liver and muscle. In conclusion, our study demonstrated that full-fat TM inclusion up to 15% in S. senegalensis diets had no negative effects or even some positive effects on fish survival, growth performance, nutrient utilization and flesh quality.
Species Richness and Composition of Forest Birds in Urban Parks and Reserves of Buenos Aires City, Argentina
Authors: Godoy Ianina N. , Gorleri Fabricio C. , Cristaldi Maximiliano A. , Leveau Lucas M.
Published: February 2024

Urbanization is among the main factors of ecosystem transformation and threats to global biodiversity. Urban green spaces provide multiple services, being important for biodiversity and human well-being. However, the relationship between green spaces and forest birds has been scarcely studied in the Global South. In this work, we used citizen science data (eBird) to assess the variation in the species richness and composition of forest birds in two types of public urban green spaces characterized by different vegetation composition and management: parks and reserves. In general, reserves had more native and unmanaged vegetation than parks. We selected parks and reserves located in the coastal area of the Metropolitan Area of Buenos Aires, Argentina. Sampling effort was considered as the number of checklists for each site. The database allowed information to be extracted from 12 sites and 33 species. The most common species were the Green-barred Woodpecker (Colaptes melanochloros), the Narrow-billed Woodcreeper (Lepidocolaptes angustirostris), and the White-crested Tyrannulet (Serpophaga subcristata). Bird species richness was higher in reserves than in parks and was positively related to sampling effort. The forest bird species composition varied according to the type of green area and sampling effort. Species composition showed a significant nestedness, with the least rich sites being a subset of species from the richest sites. Reserves and sites with the highest sampling effort concentrated all species. The results obtained show the importance of urban reserves in the conservation of forest birds.