bluuebunny/crossref_metadata_2025 huggingface dataset:
3′quant mRNA-Seq of Porcine Liver Reveals Alterations in UPR, Acute Phase Response, and Cholesterol and Bile Acid Metabolism in Response to Different Dietary Fats
Authors: Oczkowicz Maria , Szmatoła Tomasz , Świątkiewicz Małgorzata , Koseniuk Anna , Smołucha Grzegorz , Witarski Wojciech , Wierzbicka Alicja
Published: September 2020

Animal fats are considered to be unhealthy, in contrast to vegetable fats, which are rich in unsaturated fatty acids. However, the use of some fats, such as coconut oil, is still controversial. In our experiment, we divided experimental animals (domestic pigs) into three groups differing only in the type of fat used in the diet: group R: rapeseed oil (n = 5); group B: beef tallow (n = 5); group C: coconut oil (n = 6). After transcriptomic analysis of liver samples, we identified 188, 93, and 53 DEGs (differentially expressed genes) in R vs. B, R vs. C, and B vs. C comparisons, respectively. Next, we performed a functional analysis of identified DEGs with String and IPA software. We observed the enrichment of genes engaged in the unfolded protein response (UPR) and the acute phase response among genes upregulated in B compared to R. In contrast, cholesterol biosynthesis and cholesterol efflux enrichments were observed among genes downregulated in B when compared to R. Moreover, activation of the UPR and inhibition of the sirtuin signaling pathway were noted in C when compared to R. The most striking difference in liver transcriptomic response between C and B was the activation of the acute phase response and inhibition of bile acid synthesis in the latest group. Our results suggest that excessive consumption of animal fats leads to the activation of a cascade of mutually propelling processes harmful to the liver: inflammation, UPR, and imbalances in the biosynthesis of cholesterol and bile acids via altered organelle membrane composition. Nevertheless, these studies should be extended with analysis at the level of proteins and their function.
Deletion in the Bardet–Biedl Syndrome Gene TTC8 Results in a Syndromic Retinal Degeneration in Dogs
Authors: Mäkeläinen Suvi , Hellsand Minas , van der Heiden Anna Darlene , Andersson Elina , Thorsson Elina , S. Holst Bodil , Häggström Jens , Ljungvall Ingrid , Mellersh Cathryn , Hallböök Finn , Andersson Göran , Ekesten Björn , Bergström Tomas F.
Published: September 2020

In golden retriever dogs, a 1 bp deletion in the canine TTC8 gene has been shown to cause progressive retinal atrophy (PRA), the canine equivalent of retinitis pigmentosa. In humans, TTC8 is also implicated in Bardet–Biedl syndrome (BBS). To investigate if the affected dogs only exhibit a non-syndromic PRA or develop a syndromic ciliopathy similar to human BBS, we recruited 10 affected dogs to the study. The progression of PRA for two of the dogs was followed for 2 years, and a rigorous clinical characterization allowed a careful comparison with primary and secondary characteristics of human BBS. In addition to PRA, the dogs showed a spectrum of clinical and morphological signs similar to primary and secondary characteristics of human BBS patients, such as obesity, renal anomalies, sperm defects, and anosmia. We used Oxford Nanopore long-read cDNA sequencing to characterize retinal full-length TTC8 transcripts in affected and non-affected dogs, the results of which suggest that three isoforms are transcribed in the retina, and the 1 bp deletion is a loss-of-function mutation, resulting in a canine form of Bardet–Biedl syndrome with heterogeneous clinical signs.
Population Divergence along a Genetic Line of Least Resistance in the Tree Species Eucalyptus globulus
Authors: Costa e Silva João , Potts Brad M. , Harrison Peter A.
Published: September 2020

The evolutionary response to selection depends on the distribution of genetic variation in traits under selection within populations, as defined by the additive genetic variance-covariance matrix (G). The structure and evolutionary stability of G will thus influence the course of phenotypic evolution. However, there are few studies assessing the stability of G and its relationship with population divergence within foundation tree species. We compared the G-matrices of Mainland and Island population groups of the forest tree Eucalyptus globulus, and determined the extent to which population divergence aligned with within-population genetic (co)variation. Four key wood property traits exhibiting signals of divergent selection were studied—wood density, extractive content, and lignin content and composition. The comparison of G-matrices of the mainland and island populations indicated that the G-eigenstructure was relatively well preserved at an intra-specific level. Population divergence tended to occur along a major direction of genetic variation in G. The observed conservatism of G, the moderate evolutionary timescale, and close relationship between genetic architecture and population trajectories suggest that genetic constraints may have influenced the evolution and diversification of the E. globulus populations for the traits studied. However, alternative scenarios, including selection aligning genetic architecture and population divergence, are discussed.
Are Individuals Perceived as More Attractive within a Group? A Confirmative Study of Group Attractiveness Effect and the Cheerleader Effect in China
Authors: Peng Chuanyu , Mao Yanhui , Pagliaro Stefano , Roberts Scott , Livi Stefano
Published: September 2020

The stereotype “what is beautiful is good” suggests that having an attractive physical appearance is very important to people’s lives. Physical attractiveness, as an important personal trait, plays vital roles for shaping one’s first impression, and for influencing one’s subsequent evaluation and selection, based on obvious visual features. However, the contextual difference of the physical attractiveness between a group and its group members has been given less attention. For this reason, Van Osch et al. proposed and tested that the perceived physical attractiveness of a group is greater than the average attractiveness of its members (GA-effect), while Walker and Vul found that an individual as a group member is perceived to be more attractive in group context than in isolation (cheerleader effect). Our present work seeks to replicate these two effects on a sample of 1005 Chinese participants to check whether such effects also exist in China, a collectivist culture. Participants were asked to rate the degree of attractiveness presented in each photo stimuli and in each rating condition, and the results show that: (a) the participants’ ratings of physical attractiveness of a group was significantly higher than the average attractiveness of its members (i.e., replicating the GA-effect); (b) the participants’ ratings of physical attractiveness of an individual was evaluated higher in a group than in isolation (i.e., replicating the cheerleader effect); and (c) the larger the group size, the larger the GA-effect. This validating study may aid in understanding human behavior, judgments, and perceptions according to the GA-effect and the cheerleader effect used in a different context in China.
Proposing a Framework for the Restorative Effects of Nature through Conditioning: Conditioned Restoration Theory
Authors: Egner Lars Even , Sütterlin Stefan , Calogiuri Giovanna
Published: September 2020

Natural environments have been shown to trigger psychological and physiological restoration in humans. A new framework regarding natural environments restorative properties is proposed. Conditioned restoration theory builds on a classical conditioning paradigm, postulating the occurrence of four stages: (i) unconditioned restoration, unconditioned positive affective responses reliably occur in a given environment (such as in a natural setting); (ii) restorative conditioning, the positive affective responses become conditioned to the environment; (iii) conditioned restoration, subsequent exposure to the environment, in the absence of the unconditioned stimulus, retrieves the same positive affective responses; and (iv) stimulus generalization, subsequent exposure to associated environmental cues retrieves the same positive affective responses. The process, hypothetically not unique to natural environments, involve the well-documented phenomenon of conditioning, retrieval, and association and relies on evaluative conditioning, classical conditioning, core affect, and conscious expectancy. Empirical findings showing that restoration can occur in non-natural environments and through various sensory stimuli, as well as findings demonstrating that previous negative experience with nature can subsequently lower restorative effects, are also presented in support of the theory. In integration with other existing theories, the theory should prove to be a valuable framework for future research.
Impact on Mental Health Due to COVID-19 Pandemic: Cross-Sectional Study in Portugal and Brazil
Authors: Passos Lígia , Prazeres Filipe , Teixeira Andreia , Martins Carlos
Published: September 2020

Mental health effects secondary to the COVID-19 pandemic were till recently considered less important or were neglected. Portugal and Brazil are facing the pandemic in quite different ways. This study aimed to describe the mental health status of the general adult population in Portugal and Brazil during the COVID-19 pandemic and analyze the differences between the two countries. A cross-sectional quantitative study was based on an online questionnaire. Socio-demographic data were collected in addition to four validated scales: CAGE (acronym cut-annoyed-guilty-eye) Questionnaire, Satisfaction with Life Scale, Generalized Anxiety Disorder-7 and Patient Health Questionnaire-2. For each outcome, a multiple linear regression was performed. Five hundred and fifty people answered the questionnaire (435 women). The median age was 38 (Q1, Q3: 30, 47) years, 52.5% resided in Brazil and 47.5% in Portugal. The prevalence of anxiety was 71.3% (mild anxiety was present in 43.1%), the prevalence of depression was 24.7% and 23.8% of the sample had both depression and anxiety. Isolation was a significant factor for depression but not for anxiety. Well-being was below average. Mental illness was considerably higher than pre-COVID-19 levels. Portugal and Brazil will have to be prepared for future consequences of poor mental health and contribute immediate psychological support to their adult populations.
Body Image Relates to Exercise-Induced Antinociception and Mood Changes in Young Adults: A Randomized Longitudinal Exercise Intervention
Authors: Maurer Angelika , Deckert Sebastian , Levenig Claudia , Schörkmaier Theresa , Stangier Carolin , Attenberger Ulrike , Hasenbring Monika , Boecker Henning
Published: September 2020

Background: An important motivation for adolescents and young adults to engage in aerobic exercise (AE) is to improve fitness, body composition and physical appearance. These parameters have an impact on bodily perception as conceptualized by the ‘body image’ (BI) construct. AE is known to have positive effects on pain perception, mood, and body image (BI). However, no study has hitherto investigated their interrelationship within one study. Methods: Participants were randomly assigned to an intervention group (IG, n = 16, 6 months of AE) or a passive control group (CG, n = 10). Frankfurt Body-Concept Scales (FKKS), Positive and Negative Affect Scale (PANAS), State and Trait Anxiety Inventory, warmth and heat pain thresholds (WPT, HPT), pain tolerance, and graded exercise test data from baseline (T0) and the end of the intervention (T6) were analyzed using a paired t-test (p < 0.05). Results: A significant increase in the BI dimension ‘physical efficacy’ was identified from T0 to T6, which correlated positively with PANAS Positive Affect Scale and HPT. Conclusion: Data in young adults undergoing AE indicate that changes in the BI sub-category ‘physical efficacy’ are closely linked with changes in positive affect and antinociception. These novel findings suggest that BI plays a role in antinociception and positive affect.
Coordination Investigation of the Economic, Social and Environmental Benefits of Urban Public Transport Infrastructure in 13 Cities, Jiangsu Province, China
Authors: He Xinghong , Cao Zhichao , Zhang Silin , Liang Shumin , Zhang Yuyang , Ji Tianbo , Shi Quan
Published: September 2020

This study proposed an investigation-based multiple-criteria coordinated model to evaluate the sustainable development of urban public transport (PT) infrastructure, based on economic, social and environmental data from 2009 to 2019. The main problem with the traditional approach for assessing urban PT development is that economic and social benefits are considered individually, but also attention to environmental factors and coordination among the three issues are nearly overlooked. This leads to the likelihood of inaccuracies in the handling/assessment of sustainable development or an imbalance among the attributes in different cities. An investigation-based coordinated model was introduced in which a survey of 35 sub-criteria was conducted to derive the criteria necessary for coupling/coordination. A case study involving 13 cities in Jiangsu Province, China, illustrated the problems in coordinating PT systems and verified the efficacy of the proposed approach. With employing the entropy method, this study validated coordination of the PT infrastructure development of various cities in a balanced manner and used panel regression formulas to analyse the theoretical gap and empirical bottlenecks existing among economic, social and environmental benefits. With the findings of the study, the data-based investigation from 13 cities enabled the city planners/managers (including ones from other cities with similar urban levels) to give the individual priority between the ternary benefits, advance technology, allow big data-based informatisation and implement near-future autonomous PT vehicles.
Recent Advances in Occupational Exposure Assessment of Aerosols
Authors: Harper Martin
Published: September 2020

Exposure science is underpinned by characterization (measurement) of exposures. In this article, six recent advances in exposure characterization by sampling and analysis are reviewed as tools in the occupational exposure assessment of aerosols. Three advances discussed in detail are (1) recognition and inclusion of sampler wall deposits; (2) development of a new sampling and analytical procedure for respirable crystalline silica that allows non-destructive field analysis at the end of the sampling period; and (3) development of a new sampler to collect the portion of sub-300 nm aerodynamic diameter particles that would deposit in human airways. Three additional developments are described briefly: (4) a size-selective aerosol sampler that allows the collection of multiple physiologically-relevant size fractions; (5) a miniaturized pump and versatile sampling head to meet multiple size-selective sampling criteria; and (6) a novel method of sampling bioaerosols including viruses while maintaining viability. These recent developments are placed in the context of the historical evolution in sampling and analytical developments from 1900 to the present day. While these are not the only advances in exposure characterization, or exposure assessment techniques, they provide an illustration of how technological advances are adding more tools to our toolkit. The review concludes with a number of recommended areas for future research, including expansion of real-time and end-of-shift on-site measurement, development of samplers that operate at higher flow-rates to ensure measurement at lowered limit values, and development of procedures that accurately distinguish aerosol and vapor phases of semi-volatile substances.
Effects of Simulation Practicum Using Flipped Learning for Korean Nursing Students
Authors: Gu Minkyung , Sok Sohyune R.
Published: September 2020

This study aimed to examine the effects of simulation practicum using flipped learning on nursing competency, core basic nursing skill (subcutaneous injection) performance, self-efficacy, and learning satisfaction of Korean nursing students. This study used a quasi-experimental pretest-posttest control group design. The samples used were from 101 nursing students (Intervention 1: n = 34; Intervention 2: n = 34; Control: n = 33) over 20 years old in G provinces, Korea. For the experimental interventions, participants in the intervention group 1 were applied by simulation practicum using flipped learning, and those in intervention group 2 were applied by simulation practicum. Participants in the control group were applied by lecture-based practicum using a model. The measures were the study participants’ general characteristics survey, the nursing competency scale, the core basic nursing skill (subcutaneous injection) performance scale, the self-efficacy scale, and the learning satisfaction scale. There were statistically significant differences in nursing competency, core basic nursing skill (subcutaneous injection) performance, self-efficacy, and the learning satisfaction among the three groups. It was seen in this study that simulation practicum using flipped learning was the most effective teaching and learning method for the nursing practicum of Korean nursing students. The simulation practicum using flipped learning can be useful in providing nursing practicum to nursing students.
Adolescent Employment, Mental Health, and Suicidal Behavior: A Propensity Score Matching Approach
Authors: Shin Hyerine , Kim Kyung hee , Kim Ji-su , Lee Eunkyung
Published: September 2020

This study aimed to confirm the relationships between part-time work experience, mental health, and suicidal behavior in adolescents. The impact of part-time work in this population is a controversial topic, perhaps because of the sociocultural background-related inconsistencies in previous results. In this cross-sectional study, which involved a secondary analysis of data from the 11th–13th Korea Youth Risk Behavior Web-Based Surveys, conducted among 800 middle and high schools by the Korean government, we used propensity score matching analysis to minimize the impact of individual backgrounds on the findings concerning the relationships in question. Overall, part-time experience was significantly related to mental health problems and suicidal behavior even after eliminating background differences. Adolescents with part-time work experience had higher overall stress levels (odds ratio = 1.148; 95% confidence interval = 1.094, 1.205) than those without such experience, and more suicidal thoughts (odds ratio = 1.355; 95% confidence interval = 1.266, 1.450), suicide planning (odds ratio = 1.717; 95% confidence interval = 1.527, 1.929), and suicide attempts (odds ratio = 1.852; 95% confidence interval = 1.595, 2.151). Thus, it is important to pay increased attention to mental health and suicide-related issues in South Korean adolescents with part-time jobs.
Novel Insights on the Corpus Luteum Function: Role of Vaspin on Porcine Luteal Cell Angiogenesis, Proliferation and Apoptosis by Activation of GRP78 Receptor and MAP3/1 Kinase Pathways
Authors: Kurowska Patrycja , Mlyczyńska Ewa , Dupont Joelle , Rak Agnieszka
Published: September 2020

Formation and limited lifespan of corpus luteum (CL) are important for proper ovarian periodicity and fertility. Failed vascularization, imbalance between proliferation and apoptosis leads to luteal phase deficiency and infertility. The aim of this study was to examine the effect of vaspin on angiogenesis, apoptosis and proliferation as well as the involvement of 78-kDa glucose-regulated protein receptor (GRP78) and mitogen-activated kinase (MAP3/1) in these processes. Porcine luteal cells were incubated with vaspin (0.1–10 ng/mL) for 24 h to 72 h and then mRNA and protein expression of angiogenesis: vascular endothelial growth factor (VEGFA), fibroblast growth factor 2 (FGF2), angiopoietin 1 (ANGPT1), VEGFA receptors (VEGFR1, VEGFR2), apoptosis: caspase 3, bcl-2-like protein 4 (BAX), B-cell lymphoma (BCL2), and proliferation: proliferating cells nuclear antigen (PCNA), cyclin A factors as well as secretion of VEGFA, FGF2, ANGT1 were measured by real-time polymerase chain reaction (PCR), immunoblotting and enzyme-linked immunosorbent assay (ELISA), respectively. Moreover, apoptosis was assessed by caspase activity using the Caspase-Glo 3/7 assay, while proliferation was by alamarBlue. We found that vaspin enhanced luteal cell angiogenesis, proliferation, and significantly decreased apoptosis. Additionally, using GRP78 siRNA and the pharmacological inhibitor of MAP3/1 (PD98059), we observed that the effect of vaspin was reversed to the control level in all investigated processes. Taken together, our results suggest that vaspin is a new regulator of female fertility by direct regulation of CL formation and maintenance of luteal cell function.
Small Molecule Inhibitors of DYRK1A Identified by Computational and Experimental Approaches
Authors: Yoon Hye Ree , Balupuri Anand , Choi Kwang-Eun , Kang Nam Sook
Published: September 2020

Dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A) is a protein kinase with diverse functions in cell regulation. Abnormal expression and activity of DYRK1A contribute to numerous human malignancies, Down syndrome, and Alzheimer’s disease. Notably, DYRK1A has been proposed as a potential therapeutic target for the treatment of diabetes because of its key role in pancreatic β-cell proliferation. Consequently, DYRK1A is an attractive drug target for a variety of diseases. Here, we report the identification of several DYRK1A inhibitors using our in-house topological water network-based approach. All inhibitors were further verified by in vitro assay.
Adenovirus Receptor Expression in Cancer and Its Multifaceted Role in Oncolytic Adenovirus Therapy
Authors: Hensen Lobke C.M. , Hoeben Rob C. , Bots Selas T.F.
Published: September 2020

Oncolytic adenovirus therapy is believed to be a promising way to treat cancer patients. To be able to target tumor cells with an oncolytic adenovirus, expression of the adenovirus receptor on the tumor cell is essential. Different adenovirus types bind to different receptors on the cell, of which the expression can vary between tumor types. Pre-existing neutralizing immunity to human adenovirus species C type 5 (HAdV-C5) has hampered its therapeutic efficacy in clinical trials, hence several adenoviral vectors from different species are currently being developed as a means to evade pre-existing immunity. Therefore, knowledge on the expression of appropriate adenovirus receptors on tumor cells is important. This could aid in determining which tumor types would benefit most from treatment with a certain oncolytic adenovirus type. This review provides an overview of the known receptors for human adenoviruses and how their expression on tumor cells might be differentially regulated compared to healthy tissue, before and after standardized anticancer treatments. Mechanisms behind the up- or downregulation of adenovirus receptor expression are discussed, which could be used to find new targets for combination therapy to enhance the efficacy of oncolytic adenovirus therapy. Additionally, the utility of the adenovirus receptors in oncolytic virotherapy is examined, including their role in viral spread, which might even surpass their function as primary entry receptors. Finally, future directions are offered regarding the selection of adenovirus types to be used in oncolytic adenovirus therapy in the fight against cancer.
Pharmacogenetics of Type 2 Diabetes—Progress and Prospects
Authors: Nasykhova Yulia A. , Tonyan Ziravard N. , Mikhailova Anastasiia A. , Danilova Maria M. , Glotov Andrey S.
Published: September 2020

Type 2 diabetes mellitus (T2D) is a chronic metabolic disease resulting from insulin resistance and progressively reduced insulin secretion, which leads to impaired glucose utilization, dyslipidemia and hyperinsulinemia and progressive pancreatic beta cell dysfunction. The incidence of type 2 diabetes mellitus is increasing worldwide and nowadays T2D already became a global epidemic. The well-known interindividual variability of T2D drug actions such as biguanides, sulfonylureas/meglitinides, DPP-4 inhibitors/GLP1R agonists and SGLT-2 inhibitors may be caused, among other things, by genetic factors. Pharmacogenetic findings may aid in identifying new drug targets and obtaining in-depth knowledge of the causes of disease and its physiological processes, thereby, providing an opportunity to elaborate an algorithm for tailor or precision treatment. The aim of this article is to summarize recent progress and discoveries for T2D pharmacogenetics and to discuss the factors which limit the furthering accumulation of genetic variability knowledge in patient response to therapy that will allow improvement the personalized treatment of T2D.
Stearoyl-CoA Desaturase 1 Activity Determines the Maintenance of DNMT1-Mediated DNA Methylation Patterns in Pancreatic β-Cells
Authors: Dobosz Aneta M. , Janikiewicz Justyna , Borkowska Anna M. , Dziewulska Anna , Lipiec Ewelina , Dobrzyn Pawel , Kwiatek Wojciech M. , Dobrzyn Agnieszka
Published: September 2020

Metabolic stress, such as lipotoxicity, affects the DNA methylation profile in pancreatic β-cells and thus contributes to β-cell failure and the progression of type 2 diabetes (T2D). Stearoyl-CoA desaturase 1 (SCD1) is a rate-limiting enzyme that is involved in monounsaturated fatty acid synthesis, which protects pancreatic β-cells against lipotoxicity. The present study found that SCD1 is also required for the establishment and maintenance of DNA methylation patterns in β-cells. We showed that SCD1 inhibition/deficiency caused DNA hypomethylation and changed the methyl group distribution within chromosomes in β-cells. Lower levels of DNA methylation in SCD1-deficient β-cells were followed by lower levels of DNA methyltransferase 1 (DNMT1). We also found that the downregulation of SCD1 in pancreatic β-cells led to the activation of adenosine monophosphate-activated protein kinase (AMPK) and an increase in the activity of the NAD-dependent deacetylase sirtuin-1 (SIRT1). Furthermore, the physical association between DNMT1 and SIRT1 stimulated the deacetylation of DNMT1 under conditions of SCD1 inhibition/downregulation, suggesting a mechanism by which SCD1 exerts control over DNMT1. We also found that SCD1-deficient β-cells that were treated with compound c, an inhibitor of AMPK, were characterized by higher levels of both global DNA methylation and DNMT1 protein expression compared with untreated cells. Therefore, we found that activation of the AMPK/SIRT1 signaling pathway mediates the effect of SCD1 inhibition/deficiency on DNA methylation status in pancreatic β-cells. Altogether, these findings suggest that SCD1 is a gatekeeper that protects β-cells against the lipid-derived loss of DNA methylation and provide mechanistic insights into the mechanism by which SCD1 regulates DNA methylation patterns in β-cells and T2D-relevant tissues.
Multiplex Bead Array Assay of a Panel of Circulating Cytokines and Growth Factors in Patients with Albuminuric and Non-Albuminuric Diabetic Kidney Disease
Authors: Klimontov Vadim V. , Korbut Anton I. , Orlov Nikolai B. , Dashkin Maksim V. , Konenkov Vladimir I.
Published: September 2020

A panel of cytokines and growth factors, mediating low-grade inflammation and fibrosis, was assessed in patients with type 2 diabetes (T2D) and different patterns of chronic kidney disease (CKD). Patients with long-term T2D (N = 130) were classified into four groups: no signs of CKD; estimated glomerular filtration rate (eGFR) <60 mL/min/1.73 m2 without albuminuria; albuminuria and eGFR ≥60 mL/min/1.73 m2; albuminuria and eGFR <60 mL/min/1.73 m2. Thirty healthy subjects were acted as control. Twenty-seven cytokines and growth factors were assessed in serum by multiplex bead array assay. Serum hs-CRP, urinary nephrin, podocine, and WFDC2 were measured by ELISA. Patients with T2D showed elevated IL-1Ra, IL-6, IL-17A, G-CSF, IP-10, MIP-1α, and bFGF levels; concentrations of IL-4, IL-12, IL-15, INF-γ, and VEGF were decreased. IL-6, IL-17A, G-CSF, MIP-1α, and bFGF correlated negatively with eGFR; IL-10 and VEGF demonstrated negative associations with WFDC2; no relationships with podocyte markers were found. Adjusted IL-17A and MIP-1α were predictors of non-albuminuric CKD, IL-13 predicted albuminuria with preserved renal function, meanwhile, IL-6 and hsCRP were predictors of albuminuria with eGFR decline. Therefore, albuminuric and non-albuminuric CKD in T2D patients are associated with different pro-inflammatory shifts in the panel of circulating cytokines.
Long-Term Infectious Morbidity of Premature Infants: Is There a Critical Threshold?
Authors: Davidesko Sharon , Wainstock Tamar , Sheiner Eyal , Pariente Gali
Published: September 2020

In this study, we sought to ascertain a relationship between gestational age at birth and infectious morbidity of the offspring via population-based cohort analysis comparing the long-term incidence of infectious morbidity in infants born preterm and stratified by extremity of prematurity (extreme preterm birth: 24 + 0–27 + 6, very preterm birth: 28 + 0–31 + 6, moderate to late preterm birth: 32 + 0−36 + 6 weeks of gestation, and term deliveries). Infectious morbidity included hospitalizations involving a predefined set of International Classification of Diseases 9 (ICD9) codes, as recorded in hospital records. A Kaplan–Meier survival curve compared cumulative incidence of infectious-related morbidity. A Cox proportional hazards model controlled for confounders and time to event. The study included 220,594 patients: 125 (0.1%) extreme preterm births, 784 (0.4%) very preterm births, 13,323 (6.0%) moderate to late preterm births, and 206,362 term deliveries. Offspring born preterm had significantly more infection-related hospitalizations (18.4%, 19.8%, 14.9%, and 11.0% for the aforementioned stratification, respectively, p < 0.001). Multivariate analysis found being born very or late to moderate preterm was independently associated with long-term infectious morbidity (adjusted hazard ratio (aHR) 1.5, 95% confidence interval (CI) 1.27–1.77 and aHR 1.23, 95% CI 1.17–1.3, respectively, p < 0.001). A comparable risk of long-term infectious morbidity was found in the two groups of premature births prior to 32 weeks gestation. In our population, a cutoff from 32 weeks and below demarks a significant increase in the risk of long-term infectious morbidity of the offspring.
Mortality in Patients with Diabetic Foot Ulcers: Causes, Risk Factors, and Their Association with Evolution and Severity of Ulcer
Authors: Rubio José Antonio , Jiménez Sara , Lázaro-Martínez José Luis
Published: September 2020

Background: This study reviews the mortality of patients with diabetic foot ulcers (DFU) from the first consultation with a Multidisciplinary Diabetic Foot Team (MDFT) and analyzes the main cause of death, as well as the relevant clinical factors associated with survival. Methods: Data of 338 consecutive patients referred to the MDFT center for a new DFU during the 2008–2014 period were analyzed. Follow-up: until death or until 30 April 2020, for up to 12.2 years. Results: Clinical characteristics: median age was 71 years, 92.9% had type 2 diabetes, and about 50% had micro-macrovascular complications. Ulcer characteristics: Wagner grade 1–2 (82.3%), ischemic (49.2%), and infected ulcers (56.2%). During follow-up, 201 patients died (59.5%), 110 (54.7%) due to cardiovascular disease. Kaplan—Meier curves estimated a reduction in survival of 60% with a 95% confidence interval (95% CI), (54.7–65.3) at 5 years. Cox regression analysis adjusted to a multivariate model showed the following associations with mortality, with hazard ratios (HRs) (95% CI): age, 1.07 (1.05–1.08); HbA1c value < 7% (53 mmol/mol), 1.43 (1.02–2.0); active smoking, 1.59 (1.02–2.47); ischemic heart or cerebrovascular disease, 1.55 (1.15–2.11); chronic kidney disease, 1.86 (1.37–2.53); and ulcer severity (SINBAD system) 1.12 (1.02–1.26). Conclusion: Patients with a history of DFU have high mortality. Two less known predictors of mortality were identified: HbA1c value < 7% (53 mmol/mol) and ulcer severity.
Recent Advances in Signaling Pathways Comprehension as Carcinogenesis Triggers in Basal Cell Carcinoma
Authors: Tampa Mircea , Georgescu Simona Roxana , Mitran Cristina Iulia , Mitran Madalina Irina , Matei Clara , Scheau Cristian , Constantin Carolina , Neagu Monica
Published: September 2020

Basal cell carcinoma (BCC) is the most common malignant skin tumor. BCC displays a different behavior compared with other neoplasms, has a slow evolution, and metastasizes very rarely, but sometimes it causes an important local destruction. Chronic ultraviolet exposure along with genetic factors are the most important risk factors involved in BCC development. Mutations in the PTCH1 gene are associated with Gorlin syndrome, an autosomal dominant disorder characterized by the occurrence of multiple BCCs, but are also the most frequent mutations observed in sporadic BCCs. PTCH1 encodes for PTCH1 protein, the most important negative regulator of the Hedgehog (Hh) pathway. There are numerous studies confirming Hh pathway involvement in BCC pathogenesis. Although Hh pathway has been intensively investigated, it remains incompletely elucidated. Recent studies on BCC tumorigenesis have shown that in addition to Hh pathway, there are other signaling pathways involved in BCC development. In this review, we present recent advances in BCC carcinogenesis.
Seven Years Leptospirosis Follow-Up in a Critical Care Unit of a French Metropolitan Hospital; Role of Real Time PCR for a Quick and Acute Diagnosis
Authors: Bahuaud Olivier , Pastuszka Adeline , Le Brun Cécile , Ehrmann Stephan , Lanotte Philippe
Published: September 2020

(1) Background: Leptospirosis infection can lead to multiple organ failure, requiring hospitalization in an intensive care unit for supportive care, along with initiation of an adapted antibiotic therapy. Achieving a quick diagnosis is decisive in the management of these patients. (2) Methods: We present here a review of leptospirosis cases diagnosed in the intensive care unit of our hospital over seven years. Clinical and biological data were gathered, and we compared the differences in terms of diagnostic method. (3) Results: Molecular biology method by Polymerase Chain Reaction (PCR) allowed quick and reliable diagnosis when performed in the first days after the symptoms began. Moreover, we identified that sampling blood and urine for PCR was more efficient than performing PCR on only one type of biological sample. (4) Conclusions: Our results confirm the efficiency of PCR for the quick diagnosis of leptospirosis and suggest that testing both blood and urine early in the disease might improve diagnosis.
Simulation-Based Multi-Criteria Optimization of Parallel Heat Treatment Furnaces at a Casting Manufacturer
Authors: Sobottka Thomas , Kamhuber Felix , Heinzl Bernhard
Published: September 2020

This paper presents the development and evaluation of a digital method for multi-criteria optimized production planning and control of production equipment in a case-study of an Austrian metal casting manufacturer. Increased energy efficiency is a major requirement for production enterprises, especially for energy intensive production sectors such as casting. Despite the significant energy-efficiency potential through optimized planning and the acknowledged application potential for sophisticated simulation-based methods, digital tools for practical planning applications are still lacking. The authors develop a planning method featuring a hybrid (discrete-continuous) simulation-based multi-criteria optimization (a multi-stage hybrid heuristic and metaheuristic method) for a metal casting manufacturer and apply it to a heat treatment process, that requires order batching and sequencing/scheduling on parallel machines, considering complex restrictions. The results show a ~10% global goal optimization potential, including traditional business goals and energy efficiency, with a ~6% energy optimization. A basic feasibility demonstration of applying the method to synchronize energy demand with fluctuating supply by considering flexible energy prices is conducted. The method is designed to be included in the planning loop of metal casting companies: receiving orders, machine availability, temperature data and (optional) current energy market price-data as input and returning an optimized plan to the production-IT systems for implementation.
An Exploratory Association Analysis of ABCB1 rs1045642 and ABCB1 rs4148738 with Non-Major Bleeding Risk in Atrial Fibrillation Patients Treated with Dabigatran or Apixaban
Authors: Roşian Adela-Nicoleta , Iancu Mihaela , Trifa Adrian Pavel , Roşian Ştefan Horia , Mada Cristina , Gocan Cornelia Paula , Niţă Teodora , Istratoaie Sabina , Boarescu Paul-Mihai , Buzoianu Anca Dana
Published: September 2020

(1) Background: The approach of bleeding complications in patients treated with non-vitamin K oral anticoagulants (NOACs) represents an important issue in clinical practice. Both dabigatran and apixaban are substrates for P-glycoprotein and, therefore, ABCB1 gene variations may be useful in individualizing NOACs treatment, especially in high-risk patients. (2) Methods: ABCB1 rs1045642 and rs4148738 were determined in 218 atrial fibrillation patients treated with dabigatran or apixaban (70.94 ± 9.04 years; 51.83% men). (3) Results: Non-major bleeding appeared in 7.34% NOACs–treated patients. The logistic tested models based on the four genetic models revealed no significant association between the variant genotype of two ABCB1 SNPs and the risk of bleeding (p > 0.05). Among the four two-locus haplotypes, TA and CA haplotypes had the highest frequency in NOACs-treated patients with bleeding, involving a possible positive association with the susceptibility of bleeding complications (OR = 1.04 and OR = 1.91, respectively). The logistic model found no significant association of estimated haplotypes with bleeding (p > 0.05) except for the TG haplotype which had a trend toward statistical significance (p = 0.092). Among the risk factors for bleeding, only age > 70 years and stroke/TIA showed a tendency toward statistical significance. (4) Conclusions: We found no significant associations between the studied ABCB1 variant genotypes with non-major bleeding risk in NOACs-treated patients. A trend of association between TG haplotype with bleeding risk was observed, implying a protective role of this haplotype against bleeding in patients treated with dabigatran or apixaban.
Effect of Fibres on the Failure Mechanism of Composite Tubes under Low-Velocity Impact
Authors: Xiao Jie , Shi Han , Tao Lei , Qi Liangliang , Min Wei , Zhang Hui , Yu Muhuo , Sun Zeyu
Published: September 2020

Filament-wound composite tubular structures are frequently used in transmission systems, pressure vessels, and sports equipment. In this study, the failure mechanism of composite tubes reinforced with different fibres under low-velocity impact (LVI) and the radial residual compression performance of the impacted composite tubes were investigated. Four fibres, including carbon fiber-T800, carbon fiber-T700, basalt fibre, and glass fibre, were used to fabricate the composite tubes by the winding process. The internal matrix/fibre interface of the composite tubes before the LVI and their failure mechanism after the LVI were investigated by scanning electric microscopy and X-ray micro-computed tomography, respectively. The results showed that the composite tubes mainly fractured through the delamination and fibre breakage damage under the impact of 15 J energy. Delamination and localized fibre breakage occur in the glass fibre-reinforced composite (GFRP) and basalt fibre-reinforced composite (BFRP) tubes when subjected to LVI. While fibre breakage damage occurs globally in the carbon fibre-reinforced composite (CFRP) tubes. The GFRP tube showed the best impact resistance among all the tubes investigated. The basalt fibre-reinforced composite (BFRP) tube exhibited the lowest structural impact resistance. The impact resistance of the CFRP-T700 and CFRP-T800 tube differed slightly. The radial residual compression strength (R-RCS) of the BFRP tube is not sensitive to the impact, while that of the GFRP tube is shown to be highly sensitive to the impact.
Green Synthesis of Lutein-Based Carbon Dots Applied for Free-Radical Scavenging within Cells
Authors: Yang Dian , Li Li , Cao Lei , Chang Zhimin , Mei Qian , Yan Ruhong , Ge Mingfeng , Jiang Chenyu , Dong Wen-Fei
Published: September 2020

Reactive oxygen species (ROS) in the body play an important role in various processes. It is well known that harmful high levels of ROS can cause many problems in living organisms in a variety of ways. One effective way to remove intracellular ROS is to use reducing materials that can enter the cell. Herein, we developed a strong reducing carbon nano-dot from a natural product, lutein, as an initial raw material. This is a hydrothermal synthesis method with the advantages of simplicity, high yield, mild reaction conditions, and environmental friendliness. The prepared carbon dots exhibit bright blue fluorescence, and have good water solubility and biocompatibility. In particular, the carbon dots can easily enter the cell and effectively remove ROS. Therefore, the carbon dots are thought to protect cells from oxidative damage by high levels of ROS.
Impact of Gadolinium on the Structure and Magnetic Properties of Nanocrystalline Powders of Iron Oxides Produced by the Extraction-Pyrolytic Method
Authors: Serga Vera , Burve Regina , Maiorov Mikhail , Krumina Aija , Skaudžius Ramūnas , Zarkov Aleksej , Kareiva Aivaras , Popov Anatoli I.
Published: September 2020

Interest in magnetic nanoparticles is primarily due to their practical use. In this work, for the production of nanocrystalline powders of pure and gadolinium doped iron oxides, the extraction-pyrolytic method (EPM) was used. As a precursor, either iron-containing extract (iron (III) caproate in caproic acid) or its mixture with gadolinium-containing extract (gadolinium (III) valerate in valeric acid) was used. The mixed precursor contained 0.5 mol %, 2.5 mol %, 12.5 mol %, 50 mol %, and 75 mol % gadolinium in relation to the iron content. The formation of iron oxide phases, depending on the preparation conditions, was investigated. According to the results obtained, it was demonstrated that the presence of more than 2.5 mol % gadolinium additive in the mixed precursor inhibits the magnetite-to-hematite transformation process during thermal treatment. Produced samples were characterized by XRD and SEM methods, and the magnetic properties were studied.
Surface-Step-Induced Magnetic Anisotropy in Epitaxial LSMO Deposited on Engineered STO Surfaces
Authors: Pawlak Jakub , Żywczak Antoni , Kanak Jarosław , Przybylski Marek
Published: September 2020

Changes in stoichiometry, temperature, strain and other parameters dramatically alter properties of LSMO perovskite. Thus, the sensitivity of LSMO may enable control of the magnetic properties of the film. This work demonstrates the capabilities of interface engineering to achieve the desired effects. Three methods of preparing STO substrates were conducted, i.e., using acid, buffer solution, and deionized water. The occurrence of terraces and their morphology depend on the preparation treatment. Terraces propagate on deposited layers and influence LSMO properties. The measurements show that anisotropy depends on the roughness of the substrate, the method of preparing the substrate, and oxygen treatment. The collected results suggest that the dipolar mechanism may be the source of LSMO anisotropy.
Design of Paracetamol Delivery Systems Based on Functionalized Ordered Mesoporous Carbons
Authors: Goscianska Joanna , Ejsmont Aleksander , Olejnik Anna , Ludowicz Dominika , Stasiłowicz Anna , Cielecka-Piontek Judyta
Published: September 2020

The oxidized ordered mesoporous carbons of cubic and hexagonal structure obtained by two templating methods (soft and hard) were applied for the first time as delivery systems for paracetamol—the most common antipyretic and analgesic drug in the world. The process of carbon oxidation was performed using an acidic ammonium persulfate solution at 60 °C for 6 h. The functionalization was found to reduce the specific surface area and pore volume of carbon materials, but it also led to an increasing number of acidic oxygen-containing functional groups. The most important element and the novelty of the presented study was the evaluation of adsorption and release ability of carbon carriers towards paracetamol. It was revealed that the sorption capacity and the drug release rate were mainly affected by the materials’ textural parameters and the total amount of surface functional groups, notably different in pristine and oxidized samples. The adsorption of paracetamol on the surface of ordered mesoporous carbons occurred according to different mechanisms: donor–acceptor complexes and hydrogen bond formation. The adsorption kinetics was assessed using pseudo-first- and pseudo-second-order models. The regression results indicated that the adsorption kinetics was more accurately represented by the pseudo-second-order model. Paracetamol was adsorbed onto the carbon materials studied following the Langmuir type isotherm. The presence of oxygen-containing functional groups on the surface of ordered mesoporous carbons enhanced the amount of paracetamol adsorbed and its release rate. The optimal drug loading capacity and expected release pattern exhibited oxidized ordered mesoporous carbon with a hexagonal structure obtained by the hard template method.
Towards Mapping Images to Text Using Deep-Learning Architectures
Authors: Onita Daniela , Birlutiu Adriana , Dinu Liviu P.
Published: September 2020

Images and text represent types of content that are used together for conveying a message. The process of mapping images to text can provide very useful information and can be included in many applications from the medical domain, applications for blind people, social networking, etc. In this paper, we investigate an approach for mapping images to text using a Kernel Ridge Regression model. We considered two types of features: simple RGB pixel-value features and image features extracted with deep-learning approaches. We investigated several neural network architectures for image feature extraction: VGG16, Inception V3, ResNet50, Xception. The experimental evaluation was performed on three data sets from different domains. The texts associated with images represent objective descriptions for two of the three data sets and subjective descriptions for the other data set. The experimental results show that the more complex deep-learning approaches that were used for feature extraction perform better than simple RGB pixel-value approaches. Moreover, the ResNet50 network architecture performs best in comparison to the other three deep network architectures considered for extracting image features. The model error obtained using the ResNet50 network is less by approx. 0.30 than other neural network architectures. We extracted natural language descriptors of images and we made a comparison between original and generated descriptive words. Furthermore, we investigated if there is a difference in performance between the type of text associated with the images: subjective or objective. The proposed model generated more similar descriptions to the original ones for the data set containing objective descriptions whose vocabulary is simpler, bigger and clearer.
Arithmetics of Vectors of Fuzzy Sets
Authors: Wu Hsien-Chung
Published: September 2020

The arithmetic operations of fuzzy sets are completely different from the arithmetic operations of vectors of fuzzy sets. In this paper, the arithmetic operations of vectors of fuzzy intervals are studied by using the extension principle and a form of decomposition theorem. These two different methodologies lead to the different types of membership functions. We establish their equivalences under some mild conditions. On the other hand, the α-level sets of addition, difference and scalar products of vectors of fuzzy intervals are also studied, which will be useful for the different usage in applications.