bluuebunny/crossref_metadata_2025 huggingface dataset:
Use of Electrofishing to Limit the Spread of a Non-Indigenous Fish Species in the Impoundment of Aoos Springs (Greece)
Authors: Ziou Athina , Douligeri Alexandra S. , Kiriazis Nikolaos , Korakis Athanasios , Petsis Nikolaos , Moutopoulos Dimitrios K. , Katselis George
Published: September 2024

The impoundment of Aoos springs was created in 1990 to facilitate hydroelectric production, and fish fauna has been enriched through the years due to deliberate or accidental fish stockings, with certain invasive alien fish species arising (i.e., Lepomis gibbosus, Carassius gibelio), some of which are ranked among the most dangerous species for global biodiversity. A three-year monitoring survey was conducting to map the nesting areas of Pumpkinseed, L. gibbosus, to assess the effectiveness of electrofishing as an active method to reduce the spread of the corresponding species and to identify the impact of the L. gibbosus on native species. The largest percentage of nests was found in areas with silty-sand bottoms (53.4%) and low vegetation. The potential nesting area was estimated at 1.97 km2 and the area of confirmed nesting was 0.33 km2. The species appeared to nest in a small area, compared to the potential nesting area. The results also indicate that a significant percentage of the target species (71.6% of the total number of species) was removed, with an average time per sampling of 1.2 ± 1 h, whereas the impact on the other native species was minimal. These results are important for designing and implementing programs for the eradication or reduction of L. gibbosus in the impoundment of Aoos springs.
Ge-Doped Boron Nitride Nanoclusters Functionalized with Amino Acids for Enhanced Binding of Bisphenols A and Z: A Density Functional Theory Study
Authors: Yu Chan-Fan , Chang Chia Ming
Published: September 2024

This study uses density functional theory to investigate boron nitride nanoclusters functionalized with amino acids for enhanced binding of bisphenols A (BPA) and Z (BPZ) to mimic the estrogen-related receptor gamma. Three categories of nanoclusters were examined: pristine B12N12, and those which were germanium-doped for boron or nitrogen. The study reveals that hydrogen bonding patterns and molecular stability are significantly influenced by the type of functional group and the specific amino acids involved. Ge-doping generally enhances the binding stability and spontaneity of the nanocluster–amino acid–bisphenol complexes, with Glu 275 emerging as the most stable binding site. The analysis of electronic properties such as energy gap, ionization potential, electron affinity, and chemical hardness before and after bisphenol binding indicates a general trend of increased reactivity, particularly in Ge-doped nanoclusters. The findings highlight the potential of these nanocluster composites in applications requiring high reactivity and electron mobility, such as pollutant removal and drug delivery.
Corrosion of Chromium Coating Fabricated on Zircaloy-4 Substrate
Authors: Golgovici Florentina , Diniași Diana , Dincă Paul Pavel , Butoi Bogdan , Demetrescu Ioana
Published: September 2024

In the nuclear industry, coated cladding is a topical problem and it is chosen as the near-term and most promising ATF (Accident-Tolerant Fuel) cladding concept. The main objective of this concept is to enhance the accident tolerance of nuclear power plants and accordingly, the performance of cladding is expected to be improved. This work assesses the corrosion performance of a Zircalloy-4 alloy coated with a thin chromium coating by MS (magnetron sputtering), tested under a CANDU (CANada Deuterium Uranium) reactor primary circuit simulated condition (LiOH solution, 10 MPa, 310 °C, pH = 10.5). The anticorrosive performance is evaluated by a gravimetric analysis, a metallographic analysis, X-ray diffraction, electronic microscopy, and electrochemical methods. A four times less gain mass was noticed compared to uncoated Zircaloy-4, indicating a smaller corrosion rate. The SEM micrographs illustrate that the coatings are still adherent, and they are keeping the initial morphological characteristics during the autoclaving process. A SEM cross-section analysis shows values of the thickness of the coatings between 0.8 and 1.46 µm. By XRD, the presence of Cr2O3 oxide is identified. Electrochemical testing confirms good stability and good corrosion performance of Cr coating over time under autoclave conditions.
Enhancing Fracture Toughness of Dental Zirconia through Incorporation of Nb into the Surface
Authors: Ban Seiji , Yasuoka Yuta
Published: September 2024

Background: Our previous study found that the addition of pentavalent cations like niobium (Nb) to yttria-stabilized zirconia increased fracture toughness but also raised the coefficient of thermal expansion (CTE), and opacity also increased undesirably. A new surface treatment is required to boost fracture toughness without altering CTE or translucency. Methods: The surfaces of pre-sintered 3 mol% yttria-stabilized tetragonal zirconia polycrystal (3Y-TZP) and 4.2 mol% yttria-stabilized partially stabilized zirconia (4.2Y-PSZ) were treated with a Nb sol solution containing Nb2O5 nanoparticles. After drying and sintering, a high-Nb-content surface layer formed with a depth of approximately 1 mm. Results: The Nb content in this surface layer matched that of a bulk material with 1 mol% Nb2O5. The tetragonality of the surface zirconia increased, enhancing the surface fracture toughness without changing the CTE or translucency. Conclusions: Adding Nb near the surface improved the fracture toughness without affecting the CTE or translucency. This method could strengthen zirconia prostheses, allowing more reliable dental restorations.
Effect of Back Plate Preheating Assistance System and Deep Rolling Process on Microstructure Defects and Axial Force Reduction of Friction Stir Welded AA6061 Joint
Authors: Insua Pinmanee , Nakkiew Wasawat , Baisukhan Adirek , Pitjamit Siwasit
Published: September 2024

This study investigates the effects of a back plate preheating assistance system and deep rolling (DR) on axial force and tunnel defects during friction stir welding (FSW). Different preheating configurations—advancing side (AS), retreating side (RS), and both sides—were examined to evaluate their impact on axial force reduction, temperature distribution, and defect minimization. Axial force measurements were taken using a dynamometer, and temperature histories were recorded with a thermal camera. The results demonstrate that a preheating temperature of 200 °C is optimal, reducing axial force by 30.24% and enhancing material flow. This temperature also facilitated deeper tool penetration, especially when preheating was applied to both sides. Preheating on the AS resulted in the smallest tunnel defects, reducing defect size by 80.15% on the RS and 96.91% on the AS compared to the non-preheated condition. While DR further reduced tunnel defects, its effectiveness was limited by the proximity of defects to the surface. These findings offer significant insights for improving the FSW process.
Experimental Study on Mechanical Properties of Recycled Aggregate Mixed Soil
Authors: Wang Xuliang , Dong Qinxi , Hu Jun , Liu Peng , Li Zetian , Chen Yongzhan , Xu Guoyang
Published: September 2024

In the context of efforts aimed at reducing carbon emissions, the utilization of recycled aggregate soil mixes for soil stabilization has garnered considerable interest. This study examines the mechanical properties of mixed soil samples, varying by dosage of a soft soil curing agent C, recycled aggregate R content, and curing duration. Mechanical evaluations were conducted using unconfined compressive strength tests (UCS), field emission scanning electron microscopy (FESEM), and laser diffraction particle size meter tests (PSD). The results indicate that the strength of the mixed soil samples first increases and then decreases with higher dosages of recycled aggregate, reaching optimal strength at a 20% dosage. Similarly, an increase in curing agent dosage enhances the strength, peaking at 20%. The maximum strength of the mixed soils is achieved at 28 days under various proportions. The introduction of the curing agent leads to the formation of a flocculent structure, as observed in FESEM, which contributes to the enhanced strength of the soil mixes. Specimens prepared with a combination of 20% R and 20% C, maintained at a constant moisture content of 20%, and cured for 28 days exhibit a balance between economic, environmental, and engineering performance.
Research Progresses on Technologies and Theory of Blanks with Variable Thicknesses
Authors: Wang Xiaogong , Wang Sai , Lu Rihuan , Xuan Yanni , Zhang Sijia , Zhang Guangji , Hu Xianlei , Liu Xianghua , Chen Liansheng
Published: September 2024

Under the background of dual carbon policy as well as energy conservation, blanks with variable thicknesses (BVTs) which act as structural components have drawn extensive attention due to their excellent strength and formability and reasonable load-bearing distribution characteristics, particularly in the field of automotive manufacturing. With these advantages, the manufacturing technologies of these plates using more efficient rolling methods have thus emerged. This article summarizes four methods and their characteristics for manufacturing plates with variable thicknesses based on rolling technology. In addition, a review is conducted on the latest research progress of the metal flow and rolling theories of existing plates with different thicknesses in the longitudinal and transverse direction.
Integrating Fuzzy C-Means Clustering and Explainable AI for Robust Galaxy Classification
Authors: Marín Díaz Gabriel , Gómez Medina Raquel , Aijón Jiménez José Alberto
Published: September 2024

The classification of galaxies has significantly advanced using machine learning techniques, offering deeper insights into the universe. This study focuses on the typology of galaxies using data from the Galaxy Zoo project, where classifications are based on the opinions of non-expert volunteers, introducing a degree of uncertainty. The objective of this study is to integrate Fuzzy C-Means (FCM) clustering with explainability methods to achieve a precise and interpretable model for galaxy classification. We applied FCM to manage this uncertainty and group galaxies based on their morphological characteristics. Additionally, we used explainability methods, specifically SHAP (SHapley Additive exPlanations) values and LIME (Local Interpretable Model-Agnostic Explanations), to interpret and explain the key factors influencing the classification. The results show that using FCM allows for accurate classification while managing data uncertainty, with high precision values that meet the expectations of the study. Additionally, SHAP values and LIME provide a clear understanding of the most influential features in each cluster. This method enhances our classification and understanding of galaxies and is extendable to environmental studies on Earth, offering tools for environmental management and protection. The presented methodology highlights the importance of integrating FCM and XAI techniques to address complex problems with uncertain data.
Parcel-Locker-Sharing Model for E-Commerce Logistics Service Providers
Authors: Pang King-Wah , Xu Jingyi , Jiang Ruixuan , Liu Ruofan
Published: September 2024

In the e-commerce last-mile delivery process, the asset operators (logistics service providers who own parcel locker facilities) support their delivery service with parcel lockers, while the non-asset operators (logistics service providers without parcel lockers) perform door-to-door delivery. Due to demand fluctuation, asset operators’ parcel-locker slots may be left vacant, while non-asset operators are stuck with the high-cost door-to-door service. The exclusiveness of parcel-locker usage reduces resource utilization and service efficiency in last-mile delivery. Therefore, this paper proposes a parcel-locker-sharing model in which these two parties share the parcel-locker capacity in last-mile delivery. The asset operator rents the unused parcel lockers to the non-asset operator by charging a rental fee,while the non-asset operator rents the parcel lockers for delivery to save logistics costs. The motivation of this alliance is to increase the profits of both parties and that of the total supply chain. This study establishes the supply-chain profit model for the parcel-locker-sharing framework and finds that the profit or loss depends on the comparison of the operation cost savings and delivery-cost savings. A numerical analysis is conducted to validate the final result. The research further suggests the optimal rental quantity and price interval. This paper is the first to study the operational mechanism of sharing the parcel locker between two distinct types of logistics service providers and to offer recommendations for industrial application.
Fractional Calculus for Non-Discrete Signed Measures
Authors: Kolokoltsov Vassili N. , Shishkina Elina L.
Published: September 2024

In this paper, we suggest a first-ever construction of fractional integral and differential operators based on signed measures including a vector-valued case. The study focuses on constructing the fractional power of the Riemann–Stieltjes integral with a signed measure, using semigroup theory. The main result is a theorem that provides the exact form of a semigroup for the Riemann–Stieltjes integral with a measure having a countable number of extrema. This article provides examples of semigroups based on integral operators with signed measures and discusses the fractional powers of differential operators with partial derivatives.
Enzymes from Fishery and Aquaculture Waste: Research Trends in the Era of Artificial Intelligence and Circular Bio-Economy
Authors: Khiari Zied
Published: September 2024

In the era of the blue bio-economy, which promotes the sustainable utilization and exploitation of marine resources for economic growth and development, the fisheries and aquaculture industries still face huge sustainability issues. One of the major challenges of these industries is associated with the generation and management of wastes, which pose a serious threat to human health and the environment if not properly treated. In the best-case scenario, fishery and aquaculture waste is processed into low-value commodities such as fishmeal and fish oil. However, this renewable organic biomass contains a number of highly valuable bioproducts, including enzymes, bioactive peptides, as well as functional proteins and polysaccharides. Marine-derived enzymes are known to have unique physical, chemical and catalytic characteristics and are reported to be superior to those from plant and animal origins. Moreover, it has been established that enzymes from marine species possess cold-adapted properties, which makes them interesting from technological, economic and sustainability points of view. Therefore, this review centers around enzymes from fishery and aquaculture waste, with a special focus on proteases, lipases, carbohydrases, chitinases and transglutaminases. Additionally, the use of fishery and aquaculture waste as a substrate for the production of industrially relevant microbial enzymes is discussed. The application of emerging technologies (i.e., artificial intelligence and machine learning) in microbial enzyme production is also presented.
Blood Coagulation Activities and Influence on DNA Condition of Alginate—Calcium Composites Prepared by Freeze-Drying Technique
Authors: Świerczyńska Małgorzata , Król Paulina , Hernández Vázquez César , Piekarska Klaudia , Woźniak Katarzyna , Juszczak Michał , Mrozińska Zdzisława , Kudzin Marcin
Published: September 2024

The aim of this research was to synthesize and characterize alginate–calcium composites using a freeze-drying method, with a focus on their potential applications in biomedicine. This study specifically explored the biochemical properties of these composites, emphasizing their role in blood coagulation and their capacity to interact with DNA. Additionally, the research aimed to assess how the cross-linking process influences the structural and chemical characteristics of the composites. Detailed analyses, including microscopic examination, surface area assessment, and atomic absorption spectrometry, yielded significant results. The objective of this study was to examine the impact of calcium chloride concentration on the calcium content in alginate composites. Specifically, the study assessed how varying concentrations of the cross-linking solution (ranging from 0.5% to 2%) influence the calcium ion saturation within the composites. This investigation is essential for understanding the physicochemical properties of the materials, including calcium content, porosity, and specific surface area. The results are intended to identify the optimal cross-linking conditions that maximize calcium enrichment efficiency while preserving the material’s structural integrity. The study found that higher calcium chloride concentrations in alginate cross-linking improve the formation of a porous structure, enhanced by two-stage freeze-drying. Increased calcium levels led to a larger surface area and pore volume, and significantly higher calcium content. Furthermore, assays of activated partial thromboplastin time (aPTT) showed a reduction in clotting time for alginate composites containing calcium ions, indicating their potential as hemostatic agents. The aPTT test showed shorter clotting times with higher calcium ion concentrations, without enhanced activation of the extrinsic clotting pathway. The developed alginate material with calcium effectively supports hemostasis and reduces the risk of infection. The study also explored the capacity of these composites to interact with and modify the structure of plasmid DNA, underscoring their potential for future biomedical applications.
Sarcosine, Trigonelline and Phenylalanine as Urinary Metabolites Related to Visceral Fat in Overweight and Obesity
Authors: Gurgel Aline Maria Cavalcante , Batista Aline Lidiane , Cavalcanti Diogo Manuel Lopes de Paiva , Magalhães Alviclér , Zantut-Wittmann Denise Engelbrecht
Published: September 2024

The objective of the present study is to analyze the urinary metabolome profile of patients with obesity and overweight and relate it to different obesity profiles. This is a prospective, cross-sectional study in which patients with a body mass index (BMI) ≥25 kg/m were selected. Anthropometric data were assessed by physical examination and body composition was obtained by bioimpedance (basal metabolic rate, body fat percentile, skeletal muscle mass, gross fat mass and visceral fat). Urine was collected for metabolomic analysis. Patients were classified according to abdominal circumference measurements between 81 and 93, 94 and 104, and >104 cm; visceral fat up to 16 kilos and less than; and fat percentiles of <36%, 36–46% and >46%. Spectral alignment of urinary metabolite signals and bioinformatic analysis were carried out to select the metabolites that stood out. NMR spectrometry was used to detect and quantify the main urinary metabolites and to compare the groups. Seventy-five patients were included, with a mean age of 38.3 years, and 72% females. The urinary metabolomic profile showed no differences in BMI, abdominal circumference and percentage of body fat. Higher concentrations of trigonelline (p = 0.0488), sarcosine (p = 0.0350) and phenylalanine (p = 0.0488) were associated with patients with visceral fat over 16 kg. The cutoff points obtained by the ROC curves were able to accurately differentiate between patients according to the amount of visceral fat: sarcosine 0.043 mg/mL; trigonelline 0.068 mg/mL and phenylalanine 0.204 mg/mL. In conclusion, higher visceral fat was associated with urinary levels of metabolites such as sarcosine, related to insulin resistance; trigonelline, related to muscle mass and strength; and phenylalanine, related to glucose metabolism and abdominal fat. Trigonelline, sarcosine and phenylalanine play significant roles in regulating energy balance and metabolic pathways essential for controlling obesity. Our findings could represent an interesting option for the non-invasive estimation of visceral fat through biomarkers related to alterations in metabolic pathways involved in the pathophysiology of obesity.
Application of Eight Machine Learning Algorithms in the Establishment of Infertility and Pregnancy Diagnostic Models: A Comprehensive Analysis of Amino Acid and Carnitine Metabolism
Authors: Zhang Rui , Zhou Lei , Hao Xiaoyan , Yang Liu , Ding Li , Xing Ruiqing , Hu Juanjuan , Wang Fengjuan , Zhai Xiaonan , Guo Yuanbing , Cai Zheng , Gao Jiawei , Yang Jun , Liu Jiayun
Published: September 2024

To explore the effects of altered amino acids (AAs) and the carnitine metabolism in non-pregnant women with infertility (NPWI), pregnant women without infertility (PWI) and infertility-treated pregnant women (ITPW) compared with non-pregnant women (NPW, control), and develop more efficient models for the diagnosis of infertility and pregnancy, 496 samples were evaluated for levels of 21 AAs and 55 carnitines using targeted high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). Three methods were used to screen the biomarkers for modeling, with eight algorithms used to build and validate the model. The ROC, sensitivity, specificity, and accuracy of the infertility diagnosis training model were higher than 0.956, 82.89, 66.64, and 82.57%, respectively, whereas those of the validated model were higher than 0.896, 77.67, 69.72, and 83.38%, respectively. The ROC, sensitivity, specificity, and accuracy of the pregnancy diagnosis training model were >0.994, 96.23, 97.79, and 97.69%, respectively, whereas those of the validated model were >0.572, 96.39, 93.03, and 94.71%, respectively. Our findings indicate that pregnancy may alter the AA and carnitine metabolism in women with infertility to match the internal environment of PWI. The developed model demonstrated good performance and high sensitivity for facilitating infertility and pregnancy diagnosis.
Congenital Sepsis with Candida albicans—A Rare Event in the Neonatal Period: Report of Two Cases and Literature Review
Authors: Teacoe Dumitru Alin , Cormoș Roxana Cristina , Toma Diana Adela , Ștef Laura , Cucerea Manuela , Muțiu Irina , Chicea Radu , Popescu Dragoș , Chicea Eugen Dan , Boicean Adrian Gheorghe , Galiș Radu , Ognean Maria Livia
Published: September 2024

Candida spp. is rarely found in neonatal early-onset sepsis (EOS) etiology. However, candidemia is associated with increased mortality and morbidity, as in late-onset sepsis. Congenital candidiasis may present as a mucocutaneous infection or, more rarely, as a systemic infection in term and preterm infants. This paper presents case reports of two cases of congenital systemic candidiasis (CSC) caused by Candida albicans and a review of the data in the literature. An electronic search of PubMed, Scopus, and Google Scholar was performed to identify publications on congenital candidiasis. Both neonates were male, born vaginally, with risk factors for congenital candidiasis. One of the infants was born at term and presented with an almost generalized maculopapular rash at birth and congenital candidemia; parenteral fluconazole was used successfully. The other infant was born prematurely at 28 weeks of gestation; blood culture, gastric aspirate, and maternal vaginal cultures sampled at birth were positive for C. albicans. Liver and kidney involvement became apparent on the third day of life, while lung involvement was clinically evident on the fourth day. Prolonged parenteral fluconazole was administered due to multiple organ involvement and persistent candidemia. Our experience with the presented cases, similar to data in the literature, suggests that CSC may occur at any gestational age, with various clinical pictures, sometimes mimicking bacterial sepsis, and even in the absence of the rash. Careful anamnesis and a high index of suspicion are important for the prompt recognition and treatment of CSC, optimizing the short- and long-term outcomes. Further research should focus on CSC to improve its diagnosis.
Longitudinal Dispersion and Hyporheic Exchange of Neutrally Buoyant Microplastics in the Presence of Waves and Currents
Authors: Nipuni Odara Merenchi Galappaththige , Waghajiani Devvan , Obersterescu George-Catalin , Pearson Jonathan
Published: September 2024

An experimental study was conducted to identify the behaviour of neutrally buoyant microplastics (specific density, 0.94) in different hydrodynamic conditions while focusing on combined wave–current conditions and the mixing across the hyporheic zone. For in-water-column microplastics, it was observed that the streamwise dispersion of neutrally buoyant microplastics is comparable to solute dye in both slow open-channel flow conditions and combined wave–current conditions. However, for in-bed microplastics, when compared to soluble tracers, the longer timespans associated with the hyporheic exchange process allowed the density effects to enhance the vertical exchange when compared to solutes.
The Efficient Separation of Apatite from Dolomite Using Fucoidan as an Eco-Friendly Depressant
Authors: Zhang Yifan , Yang Bingqiao , Deng Bing , Luo Huihua , Zhou Fang
Published: September 2024

The aim is to explore new depressants for achieving the efficient separation of apatite and dolomite. In this work, fucoidan (FD) was examined as an eco-friendly dolomite depressant to separate dolomite from apatite. The depression ability and adsorption mechanisms were investigated. The flotation results indicated that FD selectively depressed dolomite. The flotation difference between dolomite and apatite reached 70% approximately at an FD concentration of 75 mg/L. Meanwhile, the recovery and grade of P2O5 reached 89.84% and 32.88% and that of MgO decreased to 1.64% and 34.24% in the artificially mixed minerals test. Wettability, zeta potential, and Fourier transform infrared spectroscopy (FTIR) results revealed that FD tended to adsorb onto dolomite, impeding the interaction of sodium oleate (NaOL) with dolomite, but barely affected that on apatite. Microcalorimetry analysis indicated that the adsorption heat of FD on dolomite was much higher and less time was required to achieve equilibrium. X-ray photoelectron spectroscopy (XPS) results proved that the sulfonic acid radicals within FD chemically interacted with Mg atoms on dolomite while it weakly adsorbed on apatite.
Mineralogical Insights into PGM Recovery from Middle Group (1–4) Chromite Tailings
Authors: Baloyi Nomsa Precilla , Nheta Willie , Sibanda Vusumuzi , Safari Mehdi
Published: September 2024

Variations in the recovery of platinum group metals (PGMs) are often attributed to mineralogical and other natural ore-type variations. To increase the recovery of PGMs by the flotation process, a comprehensive understanding of gangue and valuable minerals is essential for optimising the extraction and processing of metals. Recoveries may be improved if the questions of how, where, and why losses occur can be answered with a certain degree of confidence. A requirement is the availability of statistically reliable mineralogical data. The PGMs of MG-1–4 chromite tailings dumps of the western limb of the Bushveld complex (BC) were studied in detail to unravel the PGMs and the nature of the platinum group minerals in the sample. Characterisation of the chromite tailings via deportment analysis revealed that the sample contained a significant amount of 3E PGM + Au (Pt, Pd, Ru, and Au) and was concentrated in the -25 µm fraction. The results of automated mineralogical analysis showed that the sample was composed of the PGE-sulphides group, comprising 63.6 vol%, PGE-sulfarsenides 10.4 vol%, PGE-arsenides 1.3 vol%, PGE-bismuth tellurides 3.3 vol%, PGMs-alloy 4.1 vol%, and Laurite comprising 17.3 vol% of the total PGE population. The sample was composed of 66.5 vol% of liberated PGMs, 0.2 vol% attached to liberated BMS, 27.3 vol% of PGMs attached to or locked within silicate or oxide gangue composite particles, 0.2 vol% of PGMs associated with BMS attached to silicate or oxide gangue particles, and a low proportion (5.8 vol%) of PGMs reported being locked within gangue or oxide particles. The majority of PGM grains observed were reported in the fast-floating category (64.4 vol%), 27.6 vol% in the slow-floating 1 category, 2.2 vol% in the slow-floating 2 category, and 5.8 vol% to the non-floating category. The results of the study revealed that the PGMs of MG 1–4 chromite tailings were liberated; however, the low liberation index (<0.2) suggested that a significant portion of PGMs remained trapped within gangue, hindering their recovery. This highlights the need for effective comminution (crushing and grinding) to achieve better liberation. The sample contained fine particles that were more prone to being lost in the tailings and to lowering recovery due to the slimes coating valuable minerals. The recovery of the PGMs from this complex’s polymetallic bodies of low-grade and complex mineralogy will be insufficient with traditional methods and thus innovation is needed. Innovation like advanced comminution, novel flotation equipment or reagents, selective leaching and bioprocessing can overcome these challenges.
Novel Autotaxin Inhibitor ATX-1d Significantly Enhances Potency of Paclitaxel—An In Silico and In Vitro Study
Authors: Rai Prateek , Clark Christopher J. , Womack Carl B. , Dearing Curtis , Thammathong Joshua , Norman Derek D. , Tigyi Gábor J. , Sen Subhabrata , Bicker Kevin , Weissmiller April M. , Banerjee Souvik
Published: September 2024

The development of drug resistance in cancer cells poses a significant challenge for treatment, with nearly 90% of cancer-related deaths attributed to it. Over 50% of ovarian cancer patients and 30–40% of breast cancer patients exhibit resistance to therapies such as Taxol. Previous literature has shown that cytotoxic cancer therapies and ionizing radiation damage tumors, prompting cancer cells to exploit the autotaxin (ATX)–lysophosphatidic acid (LPA)–lysophosphatidic acid receptor (LPAR) signaling axis to enhance survival pathways, thus reducing treatment efficacy. Therefore, targeting this signaling axis has become a crucial strategy to overcome some forms of cancer resistance. Addressing this challenge, we identified and assessed ATX-1d, a novel compound targeting ATX, through computational methods and in vitro assays. ATX-1d exhibited an IC50 of 1.8 ± 0.3 μM for ATX inhibition and demonstrated a significant binding affinity for ATX, as confirmed by MM-GBSA, QM/MM-GBSA, and SAPT in silico methods. ATX-1d significantly amplified the potency of paclitaxel, increasing its effectiveness tenfold in 4T1 murine breast carcinoma cells and fourfold in A375 human melanoma cells without inducing cytotoxic effects as a single agent.
Photo-Thermal Conversion and Raman Sensing Properties of Three-Dimensional Gold Nanostructure
Authors: Shan Feng , Huang Jingyi , Zhu Yanyan , Wei Guohao
Published: September 2024

Three-dimensional plasma nanostructures with high light–thermal conversion efficiency show the prospect of industrialization in various fields and have become a research hotspot in areas of light–heat utilization, solar energy capture, and so on. In this paper, a simple chemical synthesis method is proposed to prepare gold nanoparticles, and the electrophoretic deposition method is used to assemble large-area three-dimensional gold nanostructures (3D-GNSs). The light–thermal water evaporation monitoring and surface-enhanced Raman scattering (SERS) measurements of 3D-GNSs were performed via theoretical simulation and experiments. We reveal the physical processes of local electric field optical enhancement and the light–thermal conversion of 3D-GNSs. The results show that with the help of the efficient optical trapping and super-hydrophilic surface properties of 3D-GNSs, they have a significant effect in accelerating water evaporation, which was increased by nearly eight times. At the same time, the three-dimensional SERS substrates based on gold nanosphere particles (GNSPs) and gold nanostar particles (GNSTs) had limited sensitivities of 10−10 M and 10−12 M to R6G molecules, respectively. Therefore, 3D-GNSs show strong competitiveness in the fields of solar-energy-induced water purification and the Raman trace detection of organic molecules.
Dual-Mode Sensing of Fe(III) Based on Etching Induced Modulation of Localized Surface Plasmon Resonance and Surface Enhanced Raman Spectroscopy
Authors: Parmigiani Miriam , Albini Benedetta , Galinetto Pietro , Taglietti Angelo
Published: September 2024

Convenient, rapid, highly sensitive and on-site iron determination is important for environmental safety and human health. We developed a sensing system for the detection of Fe(III) in water based on 7-mercapto-4-methylcoumarine (MMC)-stabilized silver-coated gold nanostars (GNS@Ag@MMC), exploiting a redox reaction between the Fe(III) cation and the silver shell of the nanoparticles, which causes a severe transformation of the nanomaterial structure, reverting it to pristine GNSs. This system works by simultaneously monitoring changes in the Localized Surface Plasmon Resonance (LSPR) and Surface-Enhanced Raman Spectroscopy (SERS) spectra as a function of added Fe(III). The proposed sensing system is able to detect the Fe(III) cation in the 1.0 × 10−5–1.5 × 10−4 M range, and its selectivity of the GNS@Ag@MMC sensor toward iron has been verified monitoring the LSPR and the SERS response to other cations with a clear selectivity toward Fe(III).
Fish Consumption and DHA Supplementation during Pregnancy: Study of Gestational and Neonatal Outcomes
Authors: Gualtieri Paola , Frank Giulia , Cianci Rossella , Dominici Francesca , Mappa Ilenia , Rizzo Giuseppe , De Santis Gemma Lou , Bigioni Giulia , Di Renzo Laura
Published: September 2024

Several studies have explored the association between fish consumption during pregnancy and favorable neonatal outcomes, although some yield conflicting results. The American College of Obstetricians and Gynecologists recommends two to three servings of low-mercury fish per week for pregnant or breastfeeding women. However, fish can be a source of pollutants, like methylmercury, impacting neurological development. Conflicting studies on docosahexaenoic acid (DHA) supplementation exist in the literature, possibly due to varied supplement dosages. This survey, involving 501 women, investigated fish consumption and DHA supplement intake concerning gestational and neonatal outcomes. Notably, 92.1% of participants consumed fish weekly, with significant differences observed in gestational weight gain, birth weight, and length for those eating fish ≥3 times weekly compared to non-consumers. This study supports the recommendation for pregnant women to include fish in their diet while limiting exposure to environmental pollutants. Omega-3 fatty acid supplements are suggested to attain nutritional benefits without mercury risk.
Self-Efficacy in Nursing Competencies during Students’ Clinical Practicum: The Development of a Self-Assessment Scale
Authors: Arribas-Marín Juan , Plumed-Moreno Calixto , Hernández-Franco Vicente
Published: September 2024

The evaluation of the competencies corresponding to the different professional profiles of future nursing graduates is fundamental to their training. In this regard, students’ self-evaluation must be part of their training. This study aimed to develop and psychometrically test the Perceived Self-Efficacy in Nursing Competencies (PSENC) Scale. This study was conducted in two phases: selecting and adjusting items and assessing the instrument’s psychometric properties. A sample of 1416 students completed the scale online. Exploratory factor and confirmatory factor analyses were conducted. Inferential analysis was carried out. The exploratory factor analysis of the PSENC scale with 20 items resulted in five factors (76.3% of variance). All factors showed Cronbach’s alpha coefficients > 0.70. The confirmatory factor analysis measurement model showed satisfactory and adequate goodness-of-fit indices. The developed scale showed the psychometric adequacy and usefulness to the self-assessment of nursing students regarding their self-efficacy expectations in competencies during their clinical practicum. This study was not registered.
Depression, Loneliness and Quality of Life in Institutionalised and Non-Institutionalised Older Adults in Portugal: A Cross-Sectional Study
Authors: Silva Celso , Ferreira Rogério , Morgado Bruno , Alves Elisabete , Fonseca César
Published: September 2024

Our study aims to estimate the prevalence of depressive symptomatology among older adults and to assess their association with loneliness and quality of life according to institutionalisation status in a Portuguese sample. Background: The World Health Organisation estimates that by 2050, the world’s population over 60 will number two billion people, which poses complex challenges in terms of maintaining the mental health of older adults. The COVID-19 pandemic has increased the prevalence of depressive symptoms in this population, but the post-pandemic phase has not yet been studied much. Methods: A cross-sectional survey was carried out in 2023 among institutionalised and non-institutionalised older adults (total n = 525; institutionalised = 458; non-institutionalised = 67) who were selected by convenience sampling. The Patient Health Questionnaire (PHQ-9) was used to assess the presence of depressive symptoms, the WHOQOL-BREF to assess perceived quality of life and the Loneliness Scale (UCLA) to assess negative feelings of loneliness. Unconditional logistic regression models were fitted to compute crude adjusted odds ratios (ORs) and the respective 95% confidence intervals (95%CIs) for the association between sociodemographic, clinical and psychosocial characteristics and depressive symptomatology, according to institutionalisation status. The final model was adjusted for sex, age, QoL and feelings of loneliness. Results: Of the 525 participants, 74.6% of the non-institutionalised participants had no or minimal depressive symptoms, while 55.4% of the institutionalised participants fell into this category. Mild to moderately severe depressive symptoms were present in 25.4% of the non-institutionalised participants. 26.9% of the institutionalised participants had mild symptoms, 11.8% had moderate symptoms, 3.9% had moderately severe symptoms, and 2.0% had severe depressive symptoms. Overall, a higher quality of life was associated with lower levels of depressive symptoms. Participants describing feelings of loneliness were more likely also to present depressive symptoms (OR = 78.10; 95%CI 2.90–2106.08 and OR = 3.53; 95%CI 1.72–6.91 for non-institutionalised and institutionalised older adults, respectively), independently of institutionalisation status. Conclusions: The prevalence of depressive symptoms is high in older adults, which means that it has not decreased after the increase seen due to the COVID-19 pandemic. A lower perception of quality of life and the presence of negative feelings of loneliness are associated with the presence of depressive symptoms. These conclusions suggest that plans should be developed to intervene in the dimensions of depressive symptoms, perceived quality of life and negative feelings of loneliness.
Opportunistic Features of Non-Clostridium botulinum Strains Containing bont Gene Cluster
Authors: Grenda Tomasz , Grenda Anna , Jakubczyk Anna , Rybczyńska-Tkaczyk Kamila
Published: September 2024

The cluster of genes determining the production of botulinum toxins is an attribute of not only the Clostridium botulinum species. This cluster is also found in other members of the Clostridium genus, such as C. baratii, C. butyricum, and C. sporogenes. The occurrence of a botulinum-like cluster has also been recorded in strains of other genera, i.e., Enterococcus faecium, as well as in a Gram-negative species isolated from freshwater sediments; however, the biological activity of bont-related genes has not been noted. It can be said that the mentioned species have a dual nature. Another species with a dual nature is C. butyricum. This bacterium is a common human and animal gut commensal bacterium and is also frequently found in the environment. Although non-toxigenic strains are currently used as probiotics in Asia, other strains have been implicated in pathological conditions, such as botulism in infants or necrotizing enterocolitis in preterm neonates. Additionally, C. baratii strains are rare opportunistic pathogens associated with botulism intoxication. They have been isolated from food and soil and can be carried asymptomatically or cause botulism outbreaks in animals and humans. In addition to the mentioned clostridia, the other microorganisms considered as non-toxigenic have also been suspected of carrying botulinum cluster Gram-negative bacteria, such as Chryseobacterium piperi isolated from freshwater sediments; however, the biological activity of bont-related genes has not been noted. Additionally, Enterococcus faecium strains have been discovered carrying BoNT-related clusters (BoNT/En). Literature data regarding the heterogeneity of BoNT-producing strains indicate the requirement to reclassify C. botulinum species and other microorganisms able to produce BoNTs or possess botulinum-like gene clusters. This article aims to show the dual nature of Clostridium strains not belonging to the C. botulinum species that are sporadically able to carry bont clusters, which are usually considered saprophytic and even probiotic, and bont-like clusters in microorganisms from other genera. The aim was also to consider the genetic mechanisms of botulinum cluster expression in strains that are considered opportunistic and the microbiological safety aspects associated with their occurrence in the environment.
The Spread of Mycobacterium chimaera from Heater–Cooler Units and Infection Risk in Heart Surgery: Lessons from the Global Outbreak?
Authors: Spagnolo Anna Maria , De Giglio Osvalda , Caggiano Giuseppina , D’Agostini Francesco , Martini Mariano , Orsini Davide , La Maestra Sebastiano
Published: September 2024

Mycobacterium chimaera (MC), a member of the Mycobacterium avium complex, can cause infections in patients after open-heart surgery due to contaminated heater–cooler units (HCUs). The transmission route of HCU-related MC infection is non-inhalational, and infection can occur in patients without previously known immune deficiency. Patients may develop endocarditis of the prosthetic valve, infection of the vascular graft, and/or manifestations of disseminated mycobacterial infection (splenomegaly, arthritis, hepatitis, nephritis, myocarditis, etc.). MC infections have serious outcomes (30–50% recurrence rate, 20–67% mortality rate). In 2015, an international outbreak of M. chimaera infections among patients undergoing cardiothoracic surgeries was associated with exposure to contaminated LivaNova 3T HCUs (formerly Stöckert 3T heater–cooler system, London, United Kingdom). In response to the global outbreak, many international agencies have issued directives and recommendations in order to reduce the risk of MC infection in cardiac surgery. Whole-genome sequencing (WGS) technology can be used to describe the global spread and dynamics of MC infections, to characterize local outbreaks, and also to identify sources of infection in hospital settings. In order to minimize the risk of contamination of HCUs and reduce the risk of patient infection, it is imperative that healthcare facilities establish a program of regular cleaning and disinfection maintenance procedures as well as monitoring of the water used and the air in the operating room, in accordance with the manufacturer’s procedure.
The Effect of Chronic Immunosuppressive Regimen Treatment on Apoptosis in the Heart of Rats
Authors: Surówka Anna , Żołnierczuk Michał , Prowans Piotr , Grabowska Marta , Kupnicka Patrycja , Markowska Marta , Szlosser Zbigniew , Kędzierska-Kapuza Karolina
Published: September 2024

Chronic immunosuppressive therapy is currently the only effective method to prevent acute rejection of a transplanted organ. Unfortunately, the expected effect of treatment brings a number of grave side effects, one of the most serious being cardiovascular complications. In our study, we wanted to investigate how treatment with commonly used immunosuppressive drugs affects the occurrence of programmed cardiac cell death. For this purpose, five groups of rats were treated with different triple immunosuppressive regimens. Cardiac tissue fragments were subjected to the TUNEL assay to visualize apoptotic cells. The expression of Bcl-2 protein, Bax protein, caspase 3 and caspase 9 was also assessed. This study indicates that all immunosuppressive protocols used chronically at therapeutic doses result in an increased percentage of cells undergoing apoptosis in rat heart tissue. The greatest changes were recorded in the TMG (rats treated with tacrolimus, mycophenolate mofetil and glucocorticosteroids) and CMG (rats treated with cyclosporin A, mycophenolate mofetil and glucocorticosteroids) groups. The TRG (rats treated with rapamycin, tacrolimus and glucocorticosteroids) group showed the lowest percentage of apoptotic cells. The internal apoptosis pathway was confirmed only in the TMG group; in the remaining groups, the results indicate programmed cell death via the receptor pathway.
Novel Therapeutic Effects of Euphorbia heterophylla L. Methanol Extracts in Macular Degeneration Caused by Blue Light in A2E-Laden ARPE-19 Cells and Retina of BALB/c Mice
Authors: Seol Ayun , Kim Ji-Eun , Jin You-Jeong , Song Hee-Jin , Roh Yu-Jeong , Kim Tae-Ryeol , Park Eun-Seo , Park Ki-Ho , Park So-Hae , Uddin Muhammad Salah , Lee Sang-Woo , Choi Young-Woo , Hwang Dae-Youn
Published: September 2024

Natural products with high antioxidant activity are considered as innovative prevention strategies to effectively prevent age-related macular degeneration (AMD) in the early stage because the generation of reactive oxygen species (ROS) leading to the development of drusen is reported as an important cause of this disease. To investigate the prevention effects of the methanol extracts of Euphorbia heterophylla L. (MEE) on AMD, its effects on the antioxidant activity, inflammatory response, apoptosis pathway, neovascularization, and retinal tissue degeneration were analyzed in N-retinylidene-N-retinylethanolamine (A2E)-landed spontaneously arising retinal pigment epithelia (ARPE)-19 cells and BALB/c mice after exposure to blue light (BL). The MEE contained 10 active components and showed high free radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and nitric oxide (NO) radicals. The pretreatments of high-dose MEE remarkably suppressed the production of intracellular ROS (88.2%) and NO (25.2%) and enhanced (SOD) activity (84%) and the phosphorylation of nuclear factor erythroid 2–related factor 2 (Nrf2) in A2E + BL-treated ARPE-19 cells compared to Vehicle-treated group. The activation of the inducible nitric oxide synthase (iNOS)-induced cyclooxygenase-2 (COX-2) mediated pathway, inflammasome activation, and expression of inflammatory cytokines was significantly inhibited in A2E + BL-treated ARPE-19 cells after the MEE pretreatment. The activation of the apoptosis pathway and increased expression of neovascular proteins (36% for matrix metalloproteinase (MMP)-9) were inhibited in the MEE pretreated groups compared to the Vehicle-treated group. Furthermore, the thickness of the whole retina (31%), outer nuclear layer (ONL), inner nuclear layer (INL), and photoreceptor layer (PL) were significantly increased by the MEE pretreatment of BALB/c mice with BL-induced retinal degeneration. Therefore, these results suggest that the MEE, with its high antioxidative activity, protects against BL-induced retinal degeneration through the regulation of the antioxidative system, inflammatory response, apoptosis, and neovascularization in the AMD mouse model.