bluuebunny/crossref_metadata_2025 huggingface dataset:
Acute Coronary Syndrome with Non-Obstructive Plaque on Angiography and Features of Vulnerable Plaque on Intracoronary Optical Coherence Tomography
Authors: Dall’Orto Clarissa Campo , Ferreira Lopes Rubens Pierry , Eurípedes Lara Vilela , Pinto Filho Gilvan Vilella , da Silva Marcos Raphael
Published: October 2023

Optical coherence tomography (OCT) has a high spatial resolution and is useful in identifying coronary lesions with high-risk features (vulnerable plaques). These plaques are strongly associated with acute coronary syndrome (ACS). In this report, we present the case of a 43-year-old male patient presenting with typical chest pain that began three hours prior to admission. The patient exhibited an elevation of the ST segments of the anterior and lateral walls. Invasive stratification revealed a 40% lesion in the middle segment of the left anterior descending (LAD) artery. The patient was given optimized clinical treatment as he had a nonobstructive lesion in the LAD at the time of angiography. During the treatment, the patient continued to complain of angina on exertion. A follow-up coronary angiography, along with OCT analysis of the middle-to-moderate lesion in the LAD, revealed a plaque predominantly rich in lipids with signs of vulnerability. A percutaneous coronary intervention was performed. The patient’s recovery was uneventful, and he was discharged the day after the procedure. This case illustrates the evolution of intravascular imaging, particularly OCT, in the detection of vulnerable plaques.
A Lightweight Diabetic Retinopathy Detection Model Using a Deep-Learning Technique
Authors: Wahab Sait Abdul Rahaman
Published: October 2023

Diabetic retinopathy (DR) is a severe complication of diabetes. It affects a large portion of the population of the Kingdom of Saudi Arabia. Existing systems assist clinicians in treating DR patients. However, these systems entail significantly high computational costs. In addition, dataset imbalances may lead existing DR detection systems to produce false positive outcomes. Therefore, the author intended to develop a lightweight deep-learning (DL)-based DR-severity grading system that could be used with limited computational resources. The proposed model followed an image pre-processing approach to overcome the noise and artifacts found in fundus images. A feature extraction process using the You Only Look Once (Yolo) V7 technique was suggested. It was used to provide feature sets. The author employed a tailored quantum marine predator algorithm (QMPA) for selecting appropriate features. A hyperparameter-optimized MobileNet V3 model was utilized for predicting severity levels using images. The author generalized the proposed model using the APTOS and EyePacs datasets. The APTOS dataset contained 5590 fundus images, whereas the EyePacs dataset included 35,100 images. The outcome of the comparative analysis revealed that the proposed model achieved an accuracy of 98.0 and 98.4 and an F1 Score of 93.7 and 93.1 in the APTOS and EyePacs datasets, respectively. In terms of computational complexity, the proposed DR model required fewer parameters, fewer floating-point operations (FLOPs), a lower learning rate, and less training time to learn the key patterns of the fundus images. The lightweight nature of the proposed model can allow healthcare centers to serve patients in remote locations. The proposed model can be implemented as a mobile application to support clinicians in treating DR patients. In the future, the author will focus on improving the proposed model’s efficiency to detect DR from low-quality fundus images.
The Axial Spin of the Carotid Bifurcation
Authors: Manta Mihaela Daniela , Rusu Mugurel Constantin , Hostiuc Sorin , Vrapciu Alexandra Diana , Manta Bogdan Adrian , Jianu Adelina Maria
Published: October 2023

(1) Background: Twisted carotid bifurcations (CBs) lead to lateralized external carotid arteries (ECAs). Such variants are usually reported on a case-by-case basis. We aimed to study the anatomical possibilities of the axial spin of CB. (2) Methods: Determinations were made bilaterally on a retrospectively assessed sample of 150 cases, 88 males and 62 females. The following types of the axial spin of the CB were determined: type CK1–CB in the coronal plane, with ICA lateral of ECA; type CK3–CB in the coronal plane, with ECA lateral of ICA; the oblique type OK1, with the ECA antero-medial of ICA; the oblique type OK3a, with the ICA antero-medially; the oblique type OK3b, with the ICA postero-laterally; the sagittal type SK2a, with ECA anterior of ICA. (3) Results: In the overall group of 300 CBs, type OK1 was found in 40%, type OK3a in 1%, type OK3b in 2%, type CK1 in 9%, type CK3 in 5.67%, and type SK2a in 42.33% of the bilateral BC group. The types SK2a (46.67%) and OK1 (33.33%) prevailed on the right side. The types OK1 (46.67%) and SK2a (38%) prevailed on the left side. There was no statistically significant association between gender and left or right subtypes. A very strong symmetry existed between the left and right sides (Pearson Chi2 = 53.93 p < 0.001) for types OK1 and SK2a. Asymmetrical types were found in different bilateral combinations. (4) Conclusions: The spin of the CB is relatively symmetrical bilaterally, especially for the variants with the ECA antero-medial or anterior to ICA.
Colloid Carcinoma Arising in an Intestinal-Type Intraductal Papillary Mucinous Neoplasm with High-Grade Dysplasia Appearing as Signet-Ring Cells of the Pancreas by Serial Pancreatic Juice Aspiration Cytology: A Case Report
Authors: Tachibana Mitsuhiro , Hirota Takayoshi , Hamayasu Hideki , Takeuchi Yu , Tsukamoto Kei , Matsushita Masahiro
Published: October 2023

We report a case of colloid carcinoma (CC) arising from an intestinal-type intraductal papillary mucinous neoplasm with high-grade dysplasia (IPMNHGD) of the pancreas, diagnosed with serial pancreatic juice aspiration cytological examination (SPACE). A rapidly growing intraductal papillary mucinous neoplasm (IPMN) in a 71-year-old Japanese man accelerated his hospitalization in our institute. Clinically, a large, ruptured pancreatic cyst was suspected. Cytologically, several mucin-positive signet-ring cells were scattered in the inflammatory, necrotic, or mucinous background. Signet-ring cells in cell block specimens were immunoreactive for MUC2, MUC5AC, maspin, S100P, and claudin-18. The final cytologic diagnosis was CC arising in an intestinal-type IPMNHGD with intraperitoneal penetration. The patient died two months after an explorative laparotomy. The cytologic diagnosis was achieved through SPACE, and the presence of signet-ring cells was characteristic. Anti-claudin-18.2-specific monoclonal antibody therapy will likely be used to treat patients with IPMNHGD in the future. This case highlights the diagnostic utility of SPACE, with particular emphasis on the characteristic presence of signet-ring cells. Furthermore, it anticipates the potential use of anti-claudin-18.2- specific monoclonal antibody therapy in the management of IPMNHGD patients.
Condition Monitoring of the Torque Imbalance in a Dual-Stator Permanent Magnet Synchronous Motor for the Propulsion of a Lightweight Fixed-Wing UAV
Authors: Suti Aleksander , Di Rito Gianpietro , Mattei Giuseppe
Published: October 2023

This paper deals with the development of a model-based technique to monitor the condition of torque imbalances in a dual-stator permanent magnet synchronous motor for UAV full-electric propulsion. Due to imperfections, degradations or uncertainties, the torque split between power lines can deviate from the design, causing internal force-fighting and reduced efficiency. This study demonstrates that, by only elaborating the measurements of speed and direct/quadrature currents of the stators during motor acceleration/deceleration, online estimations of demagnetization and electrical angle misalignment can be obtained, thus permitting the evaluation of the imbalance and total torque of the system. A relevant outcome is that the technique can be used for developing both signal-based and model-based monitoring schemes. Starting from physical first-principles, a nonlinear model of the propulsion system, including demagnetization and electrical angle misalignment, is developed in order to analytically derive the relationships between monitoring inputs (currents and speed) and outputs (degradations). The model is experimentally validated using a system prototype characterized by asymmetrical demagnetization and electrical angle misalignment. Finally, the monitoring effectiveness is assessed by simulating UAV flight manoeuvres with the experimentally validated model: injecting different levels of degradations and evaluating the torque imbalance.
Fertility in Russia: A Re-Examination Using Microdata
Authors: Kumo Kazuhiro , Kechetova Anna
Published: October 2023

This paper employs the microdata of the Russia Longitudinal Monitoring Survey (RLMS-HSE) to discuss the impact of economic factors, such as household income and female wages, and subjective well-being, such as life satisfaction and health condition, on childbirth probability in Russia, which, following a continuous decline in birth rate throughout the 1990s, began to increase in the 2000s, and rose thereafter almost continuously. The following results were obtained: higher household incomes serve to encourage childbirth, while female wages are seen to act by curtailing childbirth, and when indicators such as life satisfaction and health condition are high, the likelihood of childbirth is increased significantly. Most previous research concerning the determinants of the birth rate in Russia has shown that household income has no effect at all, but the findings in this paper suggest that this may have been due to the special circumstances that existed at the beginning of the economic transformation period in the 1990s.
Impact of E-Learning Orientation, Moodle Usage, and Learning Planning on Learning Outcomes in On-Demand Lectures
Authors: Aida Saori
Published: October 2023

The growing prominence of e-learning in education has led to the need for a comprehensive understanding of the factors influencing learning outcomes. This study aims to investigate the combined effects of e-learning orientation, Moodle usage, and learning planning on learning outcomes in the context of on-demand lectures. A sample of participants from the Department of Information Science and Engineering in the Faculty of Engineering completed questionnaires related to e-learning orientation, while Moodle usage data and learning planning information were collected. Correlation, principal component, cluster, and multiple regression analysis were conducted to examine the relationships between variables and their impact on learning outcomes. The results suggest that e-learning orientation did not exert a significant influence on learning outcomes. However, Moodle usage and learning planning emerged as crucial factors. Increased engagement with Moodle, as indicated by higher clicks and utilization of its learning functionalities, was associated with improved learning outcomes. Additionally, effective learning planning, characterized by adherence to schedules and timely submissions, positively influenced learning outcomes. The results emphasize the importance of considering multiple factors, not just a single factor, for successful online learning. These findings provide valuable insights for optimizing learning outcomes in on-demand lectures.
The Neuroeducation Training of Students in the Degrees of Early Childhood and Primary Education: A Content Analysis of Public Universities in Andalusia
Authors: Peregrina Nievas Paula , Gallardo-Montes Carmen del Pilar
Published: October 2023

Educational neuroscience presents a broad view of what learning is and how and when it occurs. Consequently, neuroeducation offers improved strategies for understanding and developing the teaching–learning process. It is, therefore, essential that teachers and students are trained in neuroeducation, given the current knowledge in this field. Consequently, this study aims to reflect on the training in neuroeducation received by undergraduate students studying early childhood and primary education and to find out how the teaching of neuroeducation is approached in the universities of the eight Andalusian provinces. As methodology, a content analysis was made, which allowed us to transform qualitative data into quantitative data. The categories analysed were “Neuroeducation”, “Metacognition”, “Cognitive processes” and “Cognition”. The study plans for the 2022/2023 academic year were considered. The analysis results of these plans showed that, despite the relevance of neuroeducation in the educational context, the study plans barely addressed this subject. This suggested that the training received by future teachers was not enough to enable them to make the most of neuroscience and neuroeducation considerations.
A Whole Education Approach to Education Reform in Barbados: Effective Provision for Students with Special Educational Needs and Disabilities
Authors: Hornby Garry
Published: October 2023

Improving education provision for children with special educational needs and disabilities in Barbados is the focus of this article. It takes into account the wider historical and educational context and uses a whole education approach to conduct the analysis and propose transformation of the education system, involving three key components. First, abandoning the system of selective secondary education based on the results of a high-stakes examination, the 11 plus, and replacing it with a more equitable system of geographical zoning. Second, revamping curricula at primary and secondary schools to emphasise the development of literacy, numeracy, interpersonal and vocational skills rather than focusing mainly on preparing students for academic examinations, especially the 11 plus examination. Third, implementing comprehensive policy and procedures for the identification and effective education of children with special educational needs and disabilities in order to maximise outcomes for all students.
Close and Conflictual: How Pupil–Teacher Relationships Can Contribute to the Alienation of Pupils from Secondary School
Authors: Looker Benjamin , Kington Alison , Vickers Jason
Published: October 2023

This article presents previously unreported findings from a larger grounded theory study which explored the intersection between pupil–teacher relationships and secondary pupils who are experiencing school alienation. Mixed data were gathered, using a questionnaire exploring teachers’ perceptions of their relationships with their students, alongside semi-structured, exploratory interviews with teachers and alienated pupils. A critical realist grounded theory design was employed, identifying closeness and conflict as causative mechanisms contributing new insights into the phenomena of school alienation. This approach allowed for data to be triangulated, constantly compared, and used to verify findings. This study discovered some pupils experience a more pronounced subset of alienation, where teachers perceive their relationships with such pupils as being less close and more conflictual when compared to their peers. It also identified that these pupils place an emphasis on negative experiences early into the formation of relationships with their teachers. Such experiences are viewed as critical incidents which are difficult to forget by alienated pupils. A diagram summarising this mechanism is presented, and the paper concludes with some professional strategies for teachers to help repair the relationship and reduce pupils’ feelings of alienation.
A Fast-Lock Variable-Gain TDC-Based N/M-Ratio MDLL Clock Multiplier
Authors: Jang Chaeyoung , Kim Jongsun
Published: October 2023

A variable-gain time-to-digital converter (TDC)-based multiplying delay-locked loop (MDLL) clock multiplier featuring fast-locking and programmable N/M-ratio frequency multiplication capability is presented in this paper. The proposed all-digital programmable N/M-ratio MDLL achieves fast-locking capability by adopting a new variable-gain TDC. In conventional fixed-gain TDC-based MDLLs, the lock time increases as the value of the multiplication factor N decreases. However, the proposed variable-gain TDC can minimize the MDLL lock time by adjusting the TDC gain according to the change in N value. Implemented in a 40 nm 1.1-V CMOS process, the proposed all-digital MDLL clock multiplier generates output clock frequencies ranging from 0.65 to 3.2 GHz, with programmable N/M ratios of N = 5 to 16 and M = 1 to 8. It achieves a fast lock time of only 3 × M (=9) reference clock cycles when N/M = 10/3 at 2.0 GHz and demonstrates a simulated peak-to-peak jitter of 3.16 ps at 3.2 GHz when N/M = 16/3. Additionally, it occupies an active area of only 0.02 mm2 (=200 μm × 100 μm) and consumes a power of 2.3 mW at 1.0 GHz.
Classification Algorithm of 3D Pattern Film Using the Optimal Widths of a Histogram
Authors: Lee Jaeeun , Choi Hongseok , Kim Jongnam
Published: October 2023

A recent development in marketing tools aimed at boosting sales involves the use of three-dimensional pattern films. These films are designed to captivate customers’ interest by applying 3D patterns to the surface of products. However, ensuring the quality of these produced 3D films can be quite challenging due to factors such as low contrast and unclear layout. Furthermore, there has been a shortage of research on methods for evaluating the quality of 3D pattern films, and existing approaches have often failed to yield satisfactory results. To address this pressing issue, we propose an algorithm for classifying 3D pattern films into either ‘good’ or ‘bad’ categories. Unlike conventional segmentation algorithm or edge detection methods, our proposed algorithm leverages the width information at specific heights of the image histograms. The experimental results demonstrate a significant disparity in histogram shapes between good and bad patterns. Specifically, by comparing the widths of all images at the quintile of the histogram height, we show that it is possible to achieve a 100% accuracy in classifying patterns as either ‘good’ or ‘bad’. In comparative experiments, our proposed algorithm consistently outperformed other methods, achieving the highest classification accuracy.
A Primer on Clustering of Forest Management Units for Reliable Design-Based Direct Estimates and Model-Based Small Area Estimation
Authors: Georgakis Aristeidis , Gatziolis Demetrios , Stamatellos Georgios
Published: October 2023

This study employs clustering analysis to group forest management units using auxiliary, satellite imagery-derived height metrics and past wall-to-wall tree census data from a natural, uneven-aged forest. Initially, we conducted an exhaustive exploration to determine the optimal number of clusters k, considering a wide range of clustering schemes, indices, and two specific k ranges. The optimal k is influenced by various factors, including the minimum k considered, the selected clustering algorithm, the clustering indices used, and the auxiliary variables. Specifically, the minimum k, the Euclidean distance metric, and the clustering index were instrumental in determining the optimal cluster numbers, with algorithms exerting minimal influence. Unlike traditional validation indices, we assessed the performance of these optimally defined clusters based on direct estimates and additional criteria. Subsequently, our research introduces a twofold methodology for Small Area Estimation (SAE). The first approach focuses on aggregating forest management units at the cluster level to increase the sample size, thereby yielding reliable design-based direct estimates for key forest attributes, including growing stock volume, basal area, tree density, and mean tree height. The second approach prepares area-level data for the future application of model-based estimators, contingent on establishing a strong correlation between target and auxiliary variables. Our methodology has the potential to enhance forest inventory practices across a wide range of forests where area-level auxiliary covariates are available.
Effects of Trophic Modes on the Lipid Accumulation of Parachlorella kessleri TY
Authors: Gao Yifan , Li Yuan , Yang Yan , Feng Jia , Ji Li , Xie Shulian
Published: October 2023

Microalgae are considered to have great potential as a source of biodiesel. Currently, algae culture has three different trophic modes, i.e., autotrophic, heterotrophic, and mixotrophic, but not all kinds of algae are suitable for heterotrophic and mixotrophic cultivation. In this study, Parachlorella kessleri TY, screened from the soil of Shanxi Province, was heterotrophically and mixotrophically treated with glucose as an organic carbon source, and the physiological and biochemical levels of its growth and lipid accumulation were measured. The results showed that the highest biomass and biomass productivity (1.53 g·L−1 and 218.57 mg·L−1d−1) were attained by P. kessleri TY under mixotrophic cultivation. In comparison, the lowest (0.55 g·L−1 and 78.57 mg·L−1d−1) were attained under heterotrophic culture. Furthermore, heterotrophic and mixotrophic conditions could accumulate more lipids (total lipid contents: 39.85% and 42.92%, respectively), especially the neutral lipids. Additionally, the contents of fatty acids suitable for use as biodiesel raw materials in both heterotrophic and mixotrophic cultures increased, especially the content of C18:1. Moreover, due to the lower biomass of heterotrophic cultivation compared with that from mixotrophic cultivation, the total lipid productivity of heterotrophic conditions decreased. In summary, the conditions of mixotrophic cultivation are more conducive to the accumulation of lipids in P. kessleri TY.
Based on the Co-Evolution of lncRNAs-Microbiota and Metabolites in Rumen Epithelium to Analyze the Adaptation Characteristics of Tibetan Sheep to Nutrient Stress in the Cold Season
Authors: Liu Xiu , Guo Xinyu , Sha Yuzhu , He Yanyu , Shao Pengyang , Hu Jiang , Wang Jiqing , Li Shaobin , Hao Zhiyun
Published: October 2023

Based on the serious phenomenon of Tibetan sheep “growing strong in warm seasons and losing weight in cold seasons”, this study explores the regulation of lncRNAs, microbiota, and metabolites in the cold season adaptation of Tibetan sheep from the perspective of the co-evolution of the host genome (first genome) and microbiome (second genome). RNA-seq results showed that 172 DE lncRNAs were identified in the rumen epithelium of Tibetan sheep in warm and cold seasons, of which 87 DE lncRNAs were significantly up-regulated in cold seasons. KEGG enrichment showed that target genes of up-regulated lncRNAs were significantly enriched in TNF signaling and oxidative phosphorylation pathways. LncRNA-mRNA regulatory network indicated that DE lncRNAs were involved in nutrient stress in the cold season by targeting ATP1B2, CADPS, TLR5, and UGT1A6. Correlation analysis showed some lncRNAs were significantly correlated with acetic acid, propionic acid, butyric acid, and rumen epithelial histomorphology and had a negative correlation with Butyrivibrio-2 and Succiniclasticum (p < 0.05). In addition, differential metabolites bilirubin and lncRNAs were co-enriched in the bile secretion pathway. lncRNAs played an important role in the adaptation process of Tibetan sheep in the cold season, and mediate the host to participate in nutrient absorption, energy utilization, and immune response, indicating that the host genome and microbial genome promote Tibetan sheep to adapt to nutrient stress in the cold season through co-evolution.
Using Deep Ocean Water in the Fermentation of Antrodia cinnamomea to Boost Magnesium Ion Bioabsorption and Anti-Inflammatory Effects in the Brain of an Alzheimer’s Disease Rat Model
Authors: Xu Ting-Yu , Tzeng De-Yu , Lee Chun-Lin
Published: October 2023

Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by the deposition of amyloid β-peptide (Aβ) and subsequent oxidative inflammatory response, leading to brain damage and memory loss. This study explores the potential of Antrodia cinnamomea (AC), a Taiwan-native fungus known for its anti-inflammatory and antioxidant properties. The metabolites of AC, including dehydroeburicoic acid (DEA), 4-acetylantroquinonol B (4-AAQB), dehydrosulphurenic acid (DSA), and polysaccharides, were of particular interest. In the experiment, deep ocean water (DOW) was used to facilitate the solid-state fermentation of Antrodia cinnamomea NTTU 206 (D-AC), aiming to enhance its functional components. The impact of D-AC on the modulation of AD-related risk factors and the augmentation of cognitive abilities was subsequently evaluated in an AD rat model. This model was established via consecutive infusions of Aβ40 into the brain over a 28-day period. The administration of D-AC resulted in remarkable improvements in the rats’ reference memory, spatial probe test, and working memory. Notably, it restored the hippocampal magnesium levels by upregulating the expression of the magnesium transporter MAGT1. Concurrently, D-AC significantly downregulated the expressions of β-secretase 1 (BACE1) and the phosphorylated tau protein (p-tau), which were both implicated in AD progression. Additionally, it mitigated inflammatory responses, as suggested by the decreased levels of tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in the hippocampus and cerebral cortex. Ultimately, the ability of D-AC to restore the brain magnesium levels, attenuate inflammatory responses, and reduce hippocampal Aβ40 deposition led to significant improvements in the cognitive decline of AD rats. D-AC demonstrated a comparable efficacy with its counterpart, AC fruiting bodies (F-AC group), despite their componential differences. This study underscores the potential of D-AC, enriched through fermentation, as a novel dietary strategy for Alzheimer’s disease prevention.
Comparative Analysis of Hepatopancreas RNA-Seq of Juvenile Grass Carp (Ctenopharyngodon idella) Fed Different Starch Diets
Authors: Zhang Jingjing , Guo Xue , Han Zhen , Qu Letian , Xia Teng , Chen Xiangning , Xu Jianhe , Ding Zhujin , Wei Chaoqing , Cheng Hanliang
Published: October 2023

This study aimed to explore the effects of different starch source diets on the growth performance and hepatopancreas RNA-seq of grass carp. Juvenile grass carp (initial body weight of 39.4 ± 1.6 g) were fed diets containing 25% corn (CO), potato (PO), and wheat (WH) starch for 8 weeks, respectively. The weight gain ratio (WGR) was significantly lower, whereas the visceral somatic index (VSI) and feed conversion ratio (FCR) were significantly higher in the CO group than those in the PO and WH groups. These indicators did not significantly differ between the PO and WH groups. Hepatopancreas RNA-seq analysis showed that 536, 514, and 647 differentially expressed genes (DEGs) were screened out in the comparisons of PO vs. WH, PO vs. CO, and CO vs. WH. The DEGs were mainly enriched in the several known pathways involved in steroid biosynthesis, cell cycle, fatty acid metabolism, and fat digestion and absorption according to Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis. The major DEGs related to lipid and carbohydrate metabolism were analyzed, in which lipogenesis-related DEGs (fasn, acc1, scd1, elovl6, and me1), fat digestion and absorption-related DEGs (fabp7, apoa1, apoa4, and pla2), and glycometabolism-related DEGs (gk, g6pd, and pepck) were down-regulated in the PO group compared with those in the CO and WH groups. Conversely, steroid synthesis-related DEGs (hmgcs, fdft1, sqle, lss, cyp51, msmo1, nsdhl, ugt, cyp1b1, and cyp7a1) were up-regulated in the PO group. These results indicate that the long-term PO ingestion could modulate hepatic lipid metabolism by reducing fatty acid biosynthesis and increasing bile acid biosynthesis. PO may be healthier in contrast to CO alone, which may not be suitable as a starch source in grass carp diet.
Anti-Biofilm Activity of Laurel Essential Oil against Vibrio parahaemolyticus
Authors: Zhu Wenxiu , Liu Jiaxiu , Zou Yue , Li Shugang , Zhao Dongyun , Wang Haisong , Xia Xiaodong
Published: October 2023

Vibrio parahaemolyticus is a primary seafood-associated pathogen that could cause gastroenteritis. It can attach to various surfaces and form a biofilm, which poses serious threats to food safety. Hence, an effective strategy is urgently needed to control the biofilm formation of V. parahaemolyticus. Laurel essential oil (LEO) is used in food, pharmaceutical and other industries, and is commonly used as a flavoring agent and valuable spice in food industries. The potential antibiofilm effects of LEO against V. parahaemolyticus were examined in this study. LEO obviously reduced biofilm biomass at subinhibitory concentrations (SICs). It decreased the metabolic activity and viability of biofilm cells. Microscopic images and Raman spectrum indicted that LEO interfered with the structure and biochemical compositions of biofilms. Moreover, it also impaired swimming motility, decreased hydrophobicity, inhibited auto-aggregation and reduced attachment to different food-contact surfaces. RT-qPCR revealed that LEO significantly downregulated transcription levels of biofilm-associated genes of V. parahaemolyticus. These findings demonstrate that LEO could be potentially developed as an antibiofilm strategy to control V. parahaemolyticus biofilms in food industries.
Insights into Recent Updates on Factors and Technologies That Modulate the Glycemic Index of Rice and Its Products
Authors: Ngo Tai Van , Kunyanee Kannika , Luangsakul Naphatrapi
Published: October 2023

Rice is a staple food and energy source for half the world’s population. Due to its quick digestion and absorption in the gastrointestinal tract, rice is typically regarded as having a high or medium–high glycemic index (GI); however, this can vary depending on the variety, nutrient compositions, processing, and accompanying factors. This report included a table of the glycemic index for rice and rice products in different countries, which could give an overview and fundamental information on the recent GI of different rice varieties. In addition, latest updates about the mechanism effects of rice nutritional profiles and processing techniques on GI were also provided and discussed. The influence of state-of-the-art GI regulation methods was also evaluated. Furthermore, the effectiveness and efficiency of applied technologies were also given. Furthermore, this review offered some aspects about the potential nutraceutical application of rice that food scientists, producers, or consumers might consider. Diverse types of rice are grown under various conditions that could affect the GI of the product. The instinct nutrients in rice could show different effects on the digestion rate of its product. It also revealed that the rice product’s digestibility is process-dependent. The postprandial glucose response of the rice products could be changed by modifying processing techniques, which might produce the new less-digestive compound or the inhibition factor in the starch hydrolysis process. Because of the significant importance of rice, this paper also concluded the challenges, as well as some important aspects for future research.
Antioxidant Effects of Turbo cornutus By-Products Visceral Extract against Hydrogen Peroxide-Induced Oxidative Stress by Regulating MAPK and Akt Signaling Pathways in Vero Cells
Authors: Lee Yeon-Ji , Kim Eun-A , Kang Nalae , Park Areumi , Heo Soo-Jin
Published: October 2023

Turbo cornutus, a marine gastropod mollusk commonly called sea snail, is found along the southern coast of Korea and holds considerable importance as a marine food resource, particularly on Jeju Island, Korea. Data are scarce on the antioxidant activity of hot water extracts from T. cornutus visceral tissue. Therefore, this study was performed to evaluate the antioxidant activities of T. cornutus visceral tissue hot water extract (TVE) and the underlying mechanisms against hydrogen peroxide-induced oxidative stress in Vero cells. The amino acid composition and antioxidant effects of TVE were evaluated. Furthermore, the impact of TVE on the expression of proteins within the mitogen-activated protein kinase (MAPK) pathway is investigated. TVE showed a concentration-dependent enhancement in its scavenging activities against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals (IC50 = 1.07 ± 0.06 mg/mL) and hydrogen peroxide (IC50 = 0.33 ± 0.03 mg/mL). TVE reduced intracellular reactive oxygen species (ROS) production and maintained cell viability under H2O2-induced oxidative stress by suppressing apoptosis in Vero cells. Additionally, TVE demonstrated regulatory effects on the MAPK and protein kinase B (Akt) signaling pathways activated by H2O2. In conclusion, the findings from our study propose that TVE holds potential as a bioactive component in the formulation of functional foods.
Potential of Modification of Techno-Functional Properties and Structural Characteristics of Citrus, Apple, Oat, and Pea Dietary Fiber by High-Intensity Ultrasound
Authors: Kalla-Bertholdt Ann-Marie , Baier Anne Kathrin , Rauh Cornelia
Published: October 2023

Plant fibers are rich in dietary fiber and micronutrients but often exhibit poor functionality. Ultrasonication can affect the particle size of plant fiber, thereby influencing other techno-functional properties. Therefore, this study aimed to investigate the effects of high-intensity ultrasound on citrus, apple, oat, and pea fiber. Initially, solutions containing 1 wt% of plant fiber were homogenized using ultrasonication (amplitude 116 µm, t = 150 s, energy density = 225 kJ/L, P¯ = 325 W). Due to cavitation effects induced by ultrasound, differences in particle size and a shift in the ratio of insoluble and alcohol-insoluble fractions for dietary fiber were observed. Additionally, viscosities for citrus and apple fiber increased from 1.4 Pa·s to 84.4 Pa·s and from 1.34 Pa·s to 31.7 Pa·s, respectively, at shear rates of 100 1s. This was attributed to observed differences in the microstructure. Freeze-dried samples of purified citrus and apple fiber revealed thin and nearly transparent layers, possibly contributing to enhanced water binding capacity and, therefore, increased viscosity. Water binding capacity for citrus fiber increased from 18.2 g/g to 41.8 g/g, and a 40% increase was observed for apple fiber. Finally, ultrasound demonstrated itself be an effective technology for modifying the techno-functional properties of plant fiber, such as water binding capacity.
Thermomechanical Properties of High Oleic Palm Oil Assessed Using Differential Scanning Calorimetry, Texture Analysis, Microscopy, and Shear Rheology
Authors: Cedeno-Sanchez Victor , Perez-Santana Melissa , Mehta Devanshu , Godinez Scarlett , Gu Liwei , Miller Victoria M. , MacIntosh Andrew J.
Published: October 2023

Standard Palm Oil (SPO) is widely used as a food ingredient partially due to its unique thermophysical properties. However, the American Heart Association recommends a saturated fat consumption of <5% of the caloric intake per day. The OxG Palm hybrid yields oil known as “palm oil with a higher content of oleic acid” (HOPO), with <35% SFA and >50% oleic acid. Characterizing novel high oleic oils is the starting point to find processes that can functionalize them such as oleogelation. This study compared the thermophysical properties of HOPO to SPO using Differential Scanning Calorimetry, shear rheology, polarized light microscopy, and texture analysis to characterize the differences between these oils. HOPO had a lower onset crystallization temperature (Δ7 °C) and its rheological behavior followed similar trends to SPO; however, large viscosity offsets were observed and were correlated to differences in crystallization temperatures. The maximum peak force of SPO was an order of magnitude higher than that of HOPO. Overall similar trends between the oils were observed, but differences in firmness, crystal morphology, and viscosity were not linearly correlated with the offset in crystallization temperature. This study quantified differences between these oils that will better enable industry to use HOPO in specific applications.
Voluntary Self-Exclusion and Contingency Management for the Treatment of Problematic and Harmful Gambling in the UK: An Exploratory Study
Authors: Zolkwer Morgan B. , Dymond Simon , Singer Bryan F.
Published: October 2023

Research into self-directed methods for reducing problematic and harmful gambling is still in its infancy. One strategy that individuals use to prevent gambling involves voluntary self-exclusion (VSE) programs. For example, VSE programs can make it challenging to access betting sites or enable banks to block gambling-related transactions. Although individual VSEs can be helpful when used alone, it is unclear whether their efficacy is enhanced when combined. Furthermore, it is unknown how VSE compliance can be improved. We propose that contingency management (CM), an evidence-based strategy to incentivise abstinence, could encourage continued VSE use, promoting long-lasting recovery from problematic or harmful gambling. Here, we conducted exploratory analyses on VSE use and CM for gambling in two populations (members of the UK general population recruited and students). Participants responded favourably regarding combined VSE use. They felt that providing vouchers exchangeable for goods/services could incentivise gambling abstinence during VSE. However, some were concerned about people potentially “gaming” the system. Participants believed supplementing VSE and CM with social support could encourage abstinence. These attitudes, and recent research on treatment providers’ opinions on CM for gambling, suggest that experimental evidence should be sought to determine the efficacy of combined VSE use and CM for gambling.
Micro-Museum Quarter as an Approach in the Culture-Led Urban Regeneration of Small Shrinking Historic Cities: The Case of Sombor, Serbia
Authors: Antonić Branislav , Djukić Aleksandra , Marić Jelena
Published: October 2023

Demographic and economic shrinkage has become a common trend in the current urbanisation environment, especially for small cities in developed countries. The desired socio-economic redevelopment of these cities has been significantly affected by the functional, organisational, financial, and professional constraints caused by both shrinkage and city size. Paradoxically, this slow development has enabled better preservation of their historic cores, urban heritage, and traditional culture. Nevertheless, the aforementioned local constraints have a profound impact on sustainable urban regeneration, and successful examples are still quite rare. This research presents an inspiring case—a small museum quarter in Sombor, Serbia. Museum quarters are a relatively new concept in culture-led urban regeneration; all known examples are located in big cities. Hence, this research tries to create an innovative methodological link between two theoretical fundaments: the role of cultural heritage in shrinking small cities and its expression through a museum quarter as one of the concepts of culture-led urban regeneration. An analytical framework for the aforementioned single case study is derived by forming this link. The main findings underline that the museum quarters in shrinking small cities should be developed in a micro-format to rationally address and the limited local resources. Furthermore, in contrast to museum quarters in big cities, they should be physically detached from the main retail street to enhance their separate identity and should be internally balanced in both spatial and functional aspects, meaning that the key museum/cultural institutions are equally dispersed throughout the quarter and clearly interconnected by a pedestrian-friendly open public space.
Influence of Far-Red Intensity during the Seedling Stage on Biomass Production and Photomorphogenic Characteristics in Leafy Greens under Sole-Source Lighting
Authors: Eylands Nathan , Mattson Neil
Published: October 2023

It has been well documented that far-red radiation (FR; 700–799 nm) elicits a shade-avoidance/shade-tolerance response across a wide range of plant species. Most sole-source lighting is relatively low in FR compared to sunlight (i.e., 2% vs. 20% of photons, respectively, integrated between 400 and 799 nm). The objective of this experiment was to determine if the photomorphogenic response to FR is a useful strategy during the seedling stage to promote leaf expansion in the hopes that subsequently transplanted seedlings would increase radiation capture resulting in higher harvestable biomass. Lettuce (cv. ‘Rex’, ‘Red Oak’, and ‘Green Grand Rapids’) seedlings were exposed to 5, 10, 20, or 30 µmol·m−2·s−1 of supplemental FR for a duration of 10 d in a growth chamber for 20 h daily. During this stage, all seedlings received background light levels of 195 µmol·m−2·s−1 PAR light from white LEDs for 20 h daily. Seedlings were transplanted into a nutrient film technique (NFT) hydroponic system in a separate growth chamber with LED fixtures that supplied white light at 295 µmol·m−2·s−1 for 16 h daily (DLI = 17 mol·m−2·d−1) until they were harvested at 35 d from seeding. At transplant, fresh weight, leaf area, and plant height were significantly greater for all cultivars exposed to 30 µmol·m−2·s−1 of supplemental FR radiation compared to the 5 µmol·m−2·s−1 control. Fresh weight increased by an average of 35% under 30 µmol·m−2·s−1 FR. Mature plant dry biomass increased by 14% when seedlings were exposed to 30 µmol·m−2·s−1 of supplemental FR radiation. Increasing far-red radiation consistently increased plant growth at the seedling stage, but these increases were generally overcome by maturation.
The Therapeutic Potential of Oxyhydrogen Gas in Oncology: A Study on Epstein–Barr Virus-Immortalised B-Lymphoblastoid (TK6) Cells
Authors: Russell Grace , Thomas Adam D. , Nenov Alexander , Mannings Georgia , Hancock John T.
Published: October 2023

Cancer is a leading cause of mortality worldwide. B-cells are a keystone of the adaptive immune response and are essential for the presentation of tumor-associated antigens to various types of T-cells. Approximately 1.5% of global cancer cases, including breast and gastric carcinomas and both Hodgkin’s and non-Hodgkin’s lymphomas, are linked with prior Epstein–Barr Virus (EBV) infection. Such properties make EBV-infected lymphocytes ideal models for understanding the effect of oxyhydrogen gas on dysfunctional cell cycling. The aim of this study is to assess the effects of the direct infusion of oxyhydrogen gas on the replicative capacity of EBV-immortalised B-lymphocytes. Oxyhydrogen gas was directly infused into cell culture media. Cells were incubated in 95% air and 5% CO2 for up to 72 h. Cell enumeration was assessed with and without the addition of mitogenic growth stimuli, and subsequent cell-cycle analysis was performed. Cell enumeration: An initial trend of replicative inhibition of TK6 cells is noted with a single oxyhydrogen treatment at the 24 and 48 h time points. The daily addition of oxyhydrogen-infused media showed statistically relevant data at 24 and 48 h but not at 72 h. In mitogen-stimulated cells, a non-statistical trend of inhibition was observed at 24, 48 and 72 h. Analysis details a significant increase in DNA in the Sub G1 phase, indicating increased apoptosis.
Assessing the Spatiotemporal Patterns and Impacts of Droughts in the Orinoco River Basin Using Earth Observations Data and Surface Observations
Authors: Paredes-Trejo Franklin , Olivares Barlin O. , Movil-Fuentes Yair , Arevalo-Groening Juan , Gil Alfredo
Published: October 2023

Droughts impact the water cycle, ecological balance, and socio-economic development in various regions around the world. The Orinoco River Basin is a region highly susceptible to droughts. The basin supports diverse ecosystems and supplies valuable resources to local communities. We assess the spatiotemporal patterns and impacts of droughts in the basin using remote sensing data and surface observations. We use monthly precipitation (P), air temperature near the surface (T2M), enhanced vegetation index (EVI) derived from Earth observations, and average daily flow (Q) data to quantify drought characteristics and impacts. We also investigated the association between drought and global warming by correlating the drought intensity and the percentage of dry area with sea surface temperature (SST) anomalies in the Pacific (Niño 3.4 index), Atlantic (North Atlantic Index [NATL]), and South Atlantic Index [SATL]) oceans. We evaluate the modulating effect of droughts on the hydrological regime of the most relevant tributaries by calculating the trend and significance of the regional standardized precipitation index (SPI) and percentage area affected by dry conditions. El Niño events worsen the region’s drought conditions (SPI vs. Niño 3.4 index, r = −0.221), while Atlantic SST variability has less influence on the basin’s precipitation regime (SPI vs. NATL and SATL, r = 0.117 and −0.045, respectively). We also found that long-term surface warming trends aggravate drought conditions (SPI vs. T2M anomalies, r = −0.473), but vegetation greenness increases despite high surface temperatures (SPI vs. EVI anomalies, r = 0.284). We emphasize the irregular spatial-temporal patterns of droughts in the region and their profound effects on the ecological flow of rivers during prolonged hydrological droughts. This approach provides crucial insights into potential implications for water availability, agricultural productivity, and overall ecosystem health. Our study underlines the urgent need for adaptive management strategies to mitigate the adverse effects of droughts on ecosystems and human populations. The insights derived from our study have practical implications for developing strategies to address the impacts of droughts and ensure the protection of this ecologically significant region.
An Ideal-Type Analysis of People’s Perspectives on Care Plans Received from the Emergency Department following a Self-Harm or Suicidal Crisis
Authors: O’Keeffe Sally , Suzuki Mimi , McCabe Rose
Published: October 2023

People presenting to Emergency Departments (EDs) in a self-harm/suicidal crisis in England receive a psychosocial assessment and care plan. We aimed to construct a typology of peoples’ perspectives on crisis care plans to explore the range of experiences of care plans. Thirty-two semi-structured interviews with people who presented to EDs following a self-harm/suicidal crisis in England were analysed using an ideal-type analysis. Cases were systematically compared to form clusters of cases with similar experiences of care plans. People’s perspectives on care plans fitted into three types: (1) personalised care plans (n = 13), consisting of advice or referrals perceived as helpful; (2) generic care plans (n = 13), consisting of generic advice that the person already knew about or had already tried; and (3) did not receive a care plan (n = 6) for those who reported not receiving a care plan, or who were only provided with emergency contacts. Care planning in the ED following a suicidal/self-harm crisis was perceived as supportive if it provided realistic and personalised advice, based on what had/had not worked previously. However, many people reported not receiving a helpful care plan, as it was ill-fitted to their needs or was not considered sufficient to keep them safe, which may mean that these patients are at increased risk of repeat self-harm.