bluuebunny/crossref_metadata_2025 huggingface dataset:
Early Transcriptome Differences Between Pre-Infected and Naïve Kid Goats Infected With Haemonchus contortus
Authors: Aboshady Hadeer M. , Félicité Yoann , Hira Julien , Barbier Claude , Bambou Jean-Christophe
Published: July 2022

In small ruminant production, gastrointestinal nematode (GIN) infection is one of the major causes of economic losses. The aim of this study was to compare the abomasal mucosa transcriptome of naïve and pre-infected goats at early time points after Haemonchus contortus infection, in order to identify different pathways and upstream regulators involved in the host immune response. Naïve and pre-infected Creole kids were orally infected with 10,000 H. contortus infective larvae (L3), and abomasal mucosa was sampled at 0, 4, and 6 days post-infection (dpi). At 6 dpi, all the animals were slaughtered to perform parasite burden counts. The mean number of L4 recovered in naïve kids was more than twice as high as that recovered in the pre-infected ones (5,860 and 2,474 respectively, p < 0.001). RNA-seq analysis showed a number of differentially expressed genes (DEGs) very low for both naïve and pre-infected animals when comparing day 0 vs. day 4 post-infection. A total of 2,237 and 3,206 DEGs were identified comparing 0 vs. 6 dpi in naïve and pre-infected animals, respectively. Interestingly, only 18 DEGs were found for the comparison of pre-infected vs. naïve animals at 6 dpi. Ingenuity pathway analysis (IPA) showed that several immune responses were activated in pre-infected compared with naïve animals at 0 and 4 dpi such as Th2 and Th1 pathways, natural killer cell, B cell receptor, IL-2, and IL-15 signaling. On the other hand, both naïve and pre-infected animals showed activation for those pathways comparing 6 vs. 0 dpi, with no difference between them. A similar pattern was recorded for upstream regulator genes which were related to immunity like TNF, IL-1β, IL-2, IL-5, TGFβ1, IFNγ, TCR, IL-18, IL-6, and IL-4. Our results showed that at 0 and 4 dpi the immune response was activated toward Th1 and Th2 pathways in pre-infected kids compared to the naïve ones, however, the same immune response was developed in naïve kids as earlier as 6 dpi. We conclude that repeated H. contortus infection in kid goats induced a concomitant early activation of a Th1 and Th2 immune response resulting in the regulation of worm establishment.
Investigating the Relationship Between Cardiac Function and Insulin Sensitivity in Horses: A Pilot Study
Authors: Williams Natasha J. , Furr Martin , Navas de Solis Cristobal , Campolo Allison , Davis Michael , Lacombe Véronique A.
Published: July 2022

Metabolic syndrome in humans is commonly associated with cardiovascular dysfunction, including atrial fibrillation and left ventricular diastolic dysfunction. Although many differences exist between human and equine metabolic syndrome, both of these conditions share some degree of insulin resistance. The aims of this pilot study were to investigate the relationship between insulin sensitivity and cardiac function. Seven horses (five mares, two geldings, aged 17.2 ± 4.2 years, weight 524 ± 73 kg) underwent insulin-modified frequently sampled intravenous glucose tolerance testing to determine insulin sensitivity (mean 2.21 ± 0.03 × 10−4 L/min/mU). Standard echocardiograms were performed on each horse, including two-dimensional, M-mode, and pulse-wave tissue Doppler imaging. Pearson and Spearman correlation analyses were used to determine the association of insulin sensitivity with echocardiographic measures of cardiac function in 5 horses. Insulin sensitivity was found to be significantly correlated with peak myocardial velocity during late diastole (r = 0.89, P = 0.0419), ratio between peak myocardial velocity in early and late diastole (r = −0.92, P = 0.0263), isovolumetric relaxation time (r = −0.97, P = 0.0072), and isovolumetric contraction time (ρ = −0.90, P = 0.0374). These preliminary data suggest that decreased insulin sensitivity is correlated with alterations in both systolic and diastolic function, as measured with tissue Doppler imaging (TDI). Due to the small sample size of this study, the relationship between insulin sensitivity and myocardial function in horses requires further investigation.
Genome-Wide Analysis of microRNAs Identifies the Lipid Metabolism Pathway to Be a Defining Factor in Adipose Tissue From Different Sheep
Authors: Liu Tian-Yi , Feng Hui , Yousuf Salsabeel , Xie Ling-Li , Miao Xiang-Yang
Published: July 2022

microRNAs are a class of important non-coding RNAs, which can participate in the regulation of biological processes. In recent years, miRNA has been widely studied not only in humans and mice, but also in animal husbandry. However, compared with other livestock and poultry breeds, the study of miRNA in subcutaneous adipose tissue of sheep is not comprehensive. Transcriptome analysis of miRNAs in subcutaneous adipose tissue of Duolang sheep, and Small Tail Han sheep was performed using RNA-Seq technology. Differentially expressed miRNAs were screened between different breeds. Target genes were predicted, and then the joint analysis of candidate genes were conducted based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. Finally, the RNA-Seq data were verified by real-time quantitative polymerase chain reaction (qRT-PCR). Herein, we identified 38 differentially expressed miRNAs (9 novel miRNAs and 29 known miRNAs). In addition, a total of 854 target genes were predicted by miRanda software. GO and KEGG pathway analysis demonstrated that regulation of lipolysis in adipocytes plays a key role in the deposition of subcutaneous adipose tissue in Duolang sheep and Small Tail Han sheep. The miRNAs might regulate fat deposits by regulating genes involved in regulation of lipolysis in adipocytes. Specifically, NC_ 040278.1_ 37602, oar-mir-493-3p, NC_ 040278.1_ 37521 and NC_ 040255.1_ 11627 might target PTGS2, AKT2, AKT3, and PIK3CA, respectively, and then play critical regulatory role. In conclusion, all the results provide a good idea for further revealing the mechanism of subcutaneous adipose tissue deposition and improving the meat production performance of sheep, and lay a foundation for promoting the development of animal husbandry.
Limited Sequence Variation and Similar Phenotypic Characteristics of HIV-1 Subtype C Gag Variants Derived From the Reservoir and Pre-Therapy Plasma
Authors: Ojwach Doty , Gounder Kamini , Mulaudzi Takalani , Gumede Nombali , Baiyegunhi Omolara O. , Reddy Kavidha , Giandhari Jennifer , Dong Krista L. , Ndhlovu Zaza , Ndung’u Thumbi , Mann Jaclyn K.
Published: July 2022

HIV variants present in the reservoir, particularly in tissues, may differ from those present in peripheral blood prior to therapy initiation, and characterisation of these reservoir variants could better inform immune-based interventions for HIV cure. In the present study, Gag sequence differences between variants derived from the lymph node and peripheral blood mononuclear cell (PBMC) reservoirs as well as those derived from pre-therapy plasma, were investigated in 24 HIV-1 subtype C-infected individuals. HIVgagamplification was successful for 20 individuals, where 4 were controls including one untreated individual and 3 early treated individuals with LN collection within 2 weeks of treatment initiation. The remaining 16 individuals with LN and PBMC collection > 3 months after treatment initiation (median = 665 days), were further characterised. Recombinant viruses encoding patient-derived Gag-protease sequences from the pre-therapy plasma, LN reservoir, and PBMC reservoir, were constructed and the replication-competent viruses that grewin vitrowere used to further investigate whether there are specific features of Gag reservoir variants that may have relevance for strategies to cure HIV. Virus characteristics measured included replication capacity, interferon-alpha resistance, cell-to-cell spread ability, and induction of antiviral cytokines. A limited number of novel Gag mutations (median = 4) in the reservoir of 3/7 early treated participants and 9/9 late treated participants were observed, where the majority of these mutations were likely cytotoxic T lymphocyte (CTL)-driven and 48% were represented in the replication-competent viruses. The reservoir variants had very few unique potential CTL escape mutations (median = 3) in Gag compared to the number of these Gag mutations that were already present in the plasma-derived virus (median = 23) at the time of treatment initiation, which was similar whether treatment was initiated late or early. The data suggest that the extent of CTL escape in Gag overall is likely similar between early and late treated individuals as well as between the reservoir and pre-therapy variants. The sequence differences in Gag that were unique to the reservoir viruses did not result in significantly altered virus characteristics overall, and are therefore unlikely to affect effectiveness of immune-based interventions for virus eradication.
Addressing the Evidence Gap in the Economic and Social Benefits of Civil Registration and Vital Statistics Systems: A Systematic Review
Authors: Revenga Becedas Rebeca , Sant Fruchtman Carmen , Dincu Irina , De Savigny Donald , Cobos Muñoz Daniel
Published: July 2022

Objectives:Considering the aspiration embedded in the Sustainable Development Goals to Leave No One Behind by 2030, civil registration and vital statistics systems have an essential role in providing reliable, up-to-date information to monitor the progress. Thus, the aim of this systematic review is to compile empirical evidence on the benefits of a functioning civil registration and vital statistics system.

Methods:Selected databases were systematically searched until 2019. Key experts were also contacted for relevant literature. The review process was managed with the software EPPI-Reviewer and followed standard methods for systematic reviews.

Results:A total of 18 studies were included. The findings revealed that having birth, death, and/or marriage registration, and vital statistics were associated with access to rights and protection, positive impact on economic and health outcomes, and increased access to education.

Conclusion:The present review supports the idea that systemic approaches strengthen civil registration and vital statistics systems due to the cumulative effects of vital events’ registration. Ensuring appropriate systems for civil registration will have an impact not only on the individuals but also on the generations to come.
Optimization and Improvement of Display Interaction System Based on Complex Command and Control Tasks
Authors: Wang Wei , Hong Xuefeng , Qu Jue , Xu Ning , Chen Tong
Published: July 2022

A complex command and control task was selected as the test task, which included observing the overall and local situation, the interactive operation and situation display of detection equipment, the erection and launch of air defense equipment, and the check and display status. The disadvantages of the traditional two-dimensional display interactive system include poor intuitiveness, insufficient information display dimension and complicated interactive operation. The mixed reality display interaction system can avoid these problems well and has the advantages of good portability and high efficiency, but this display interaction system has the problem of high cognitive load. Therefore, based on the premise of completing the same complex task, how to select and improve the display interaction system has become a problem worthy of urgent research. Based on the same complex command and control task, this paper compared the traditional two-dimensional display interaction system and the mixed reality display interaction system and analyzed the performance and cognitive load of the two systems. It is concluded that when completing the same task, the performance of the mixed reality display interaction system is significantly higher than that of the traditional two-dimensional display interaction system, but the cognitive load is slightly higher than that of the traditional two-dimensional display. Cognitive load was reduced while task performance was improved through multi-channel improvements to the mixed reality display interaction system. Considering the effects of performance and cognitive load, the improved multi-channel mixed reality display interaction system is superior to the unimproved mixed reality display interaction system and the two-dimensional display interaction system. This research provides an improvement strategy for the existing display interaction system and provides a new display interaction mode for future aerospace equipment and multi-target, multi-dimensional command and control tasks in war.
Experimental Study on Droplet Characteristics of Rotating Sprinklers with Circular Nozzles and Diffuser
Authors: Wang Jian , Song Zhuoyang , Chen Rui , Yang Ting , Tian Zuokun
Published: July 2022

The characteristics of spray droplets are important for calculating the hydraulic performance of sprinklers. In order to evaluate the effects of working pressure and nozzle diameter on the near ground droplet characteristics of the Nelson R33 sprinkler, an experiment was conducted to test the droplet size and velocity by using a two-dimensional video disdrometer (2DVD). Based on the water application rate, droplet diameter and velocity, the kinetic energy was calculated. The results show that there is an exponential positive correlation between the range and the volume-weighted mean particle size of droplets (VMD). The average kinetic energy of a single droplet fits well with the power function model. Under the minimum pressure of 200 kPa, the diameter and kinetic energy of droplets are large, and the peak values are 5.67 mm and 0.0092 J, which are 1.14 to 1.62 times and 1.18 to 5.68 times those of other working conditions, respectively. When the nozzle diameter is the smallest (4.4 mm), the droplet diameter and peak kinetic energy are 1.12 to 1.58 times and 1.02 to 1.26 times higher than 4.8 and 5.2 mm. Therefore, it is not recommended to work under the condition of less than 250 kPa, and a small-diameter nozzle should be selected while ensuring uniform kinetic energy.
Integrating Ecological Principles for Addressing Plant Production Security and Move beyond the Dichotomy ‘Good or Bad’ for Nitrogen Inputs Choice
Authors: Martins-Loução Maria Amélia , Dias Teresa , Cruz Cristina
Published: July 2022

Mankind’s strong dependence on nitrogen (N) began when we started farming and, ever since, we have depended on nitrogen in the soil for plant production. More than a century has passed since the discovery of N as an element until the advent of synthetic fertilizers. Today, after a century of Haber–Bosch innovation, many other endeavors and challenges can be launched to understand how the effects of N in the environment can be perceived as ‘good’ or ‘bad’. All this knowledge evolution was truly dependent on the scientific advances, both technological and methodological, and particularly on the approaches at the micro and macro level. As with nearly everything in our lives (e.g., events, people, food, decisions, world history), we tend to use the dichotomy ‘good or bad’ to categorize, and scientific advances are no exception. The integration of scientific and technological advances allows us to move beyond this simple dichotomy ‘good or bad’ and to make choices. Here, we review the main marks in understanding plant nutrition throughout time, with special emphasis on N, from the Greeks to the most recent trends in the 21st century. Since improving plant N use efficiency is a main avenue to meet several Sustainable Developmental Goals (e.g., SDG2 zero hunger, SDG12 responsible production and consumption, SDG15 life on land), the European Green Deal, and The Farm to Fork strategy, we propose that the ecological principles must be integrated in agro-ecosystem management. During the last 40 years, our research group has contributed to: (i) the clarification of the so-called dichotomy of choices when it comes to the environmental effects of N; and (ii) fetching natural solutions for N manmade problems. This was based on the knowledge that life is a continuous symbiotic interplay between mutualism and parasitism depending on environmental conditions and that there is a need for feeding people, assuring food quality and diminishing environmental impacts. We argue that, as a society, we have the scientific and technological means to learn from nature and to apply the ecological rules in agro-ecosystems. However, this is a choice we must make as individuals and as a society.
Retrospective Genetic Analysis of Qualitative and Quantitative Traits in Sweet Watermelon (Citrullus lanatus var. lanatus): A Review
Authors: Mashilo Jacob , Shimelis Hussein , Maja Dorcus , Ngwepe Richard Mantlo
Published: July 2022

Understanding the genetic basis of a crop’s qualitative and quantitative traits is vital to designing market preferred varieties. The aim of this review is to present a retrospective genetic analysis of qualitative and quantitative phenotypic traits in sweet watermelon as a guide for trait integration and the development of novel varieties with yield potential and desirable horticultural attributes. The first section outlines genes conditioning the inheritance of plant architecture (e.g., leaf attributes and plant architecture), floral characters (flowering rate, sex expression, and male sterility), fruit traits (shape, colour, rind colour and stripe patterns and flesh colour) and seed morphology (seed length, width, size and coat colour). In the second section, developments in molecular markers and quantitative trait loci (QTL) to aid marker-assisted breeding are discussed. Further, the review highlights the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) gene-editing technology and its scope in gene manipulations and new variety development. The information presented in this review is useful for optimised and demand-led breeding to develop new varieties to serve growers, consumers and the sweet watermelon industry.
Babassu Byproducts in Total Mixed Ration Silage Based on Sugarcane for Small Ruminants Diets
Authors: Zanine Anderson , Portela Ygor , Ferreira Daniele , Parente Michelle , Parente Henrique , Santos Edson , Oliveira Juliana , Perazzo Alexandre , Nascimento Thiago , da Cunha Ivo Alexandre , de Sà Cledson , Machado Nítalo , Santos Francisco Naysson , Sousa Francisca Claudia , Costa Renata
Published: July 2022

The aim of the present study was to evaluate the fermentative profile, chemical composition, and in situ degradability of total mixed ration silage with or without babassu byproducts formulated for sheep diets. The experiment was performed in a completely randomized design, with four treatments (silages) and five replications (silos). The treatments were SS: sugarcane silage (control); SSCS: sugarcane silage with corn and soybean meal; SSBF: sugarcane silage with corn, soybean meal, and babassu flour; and SSBC: sugarcane silage with corn, soybean meal, and babassu cake. The experimental diets were formulated to be isoprotein, and to meet the nutritional requirements of confined sheep with an average weight of 20 kg, according to Nutrient Requirements of Small Ruminants. The SSCS, SSBF, and SSBC treatments presented the highest values of dry matter recovery (83.74, 82.08, and 83.92%, respectively), and higher dry matter (349.07, 344,39, and 352.32 g/kg, respectively), crude protein (151.19, 136.98, and 142.14 g/kg DM, respectively), and non-fibrous carbohydrate (444.70, 353.40, and 371.30 g/kg DM, respectively) contents than the SS treatment. The largest degradations of DM, CP, and neutral detergent fiber in each treatment occurred at 72 h, in which the SSCS treatments presented the highest degradations in comparison to the others (80.59, 87.89, and 55.68, respectively). The inclusion of babassu byproducts in sugarcane silages in the form of total ration acted positively in the qualitative indicators of the silages, improving the fermentation profile and reducing losses, as well as improving the chemical composition and in situ degradability.
Development of Nonstructural Protein-Based Indirect ELISA to Identify Elephant Endotheliotropic Herpesvirus (EEHV) Infection in Asian Elephants (Elephas maximus)
Authors: Guntawang Thunyamas , Sittisak Tidaratt , Tankaew Pallop , Thitaram Chatchote , Langkapin Varangkana , Angkawanish Taweepoke , Singhla Tawatchai , Sthitmatee Nattawooti , Hsu Wei-Li , Thanawongnuwech Roongroje , Pringproa Kidsadagon
Published: July 2022

Disease caused by elephant endotheliotropic herpesvirus (EEHV) infection is the most highly fatal hemorrhagic disease in Asian elephant calves worldwide. To date, adult elephants that have been infected with EEHV have predominantly displayed mild clinical signs, while they are believed to serve as EEHV shedders to other elephants. Hence, the diagnostic tools employed for monitoring EEHV-active infection are considered vitally important. In this study, partial EEHV-DNA polymerase (DNApol) nonstructural proteins (NSPs) were used to detect anti-EEHV antibodies through the use of an in-house indirect enzyme-linked immunosorbent assay (ELISA). The results were then compared to those obtained from a PCR test. In this study, a total of 175 serum samples were collected from Asian elephants living in elephant camps located in Chiang Mai and Lampang Provinces, Thailand. The elephants were aged between 2 and 80 years old. The overall percentages of positive samples by the PCR and EEHV-DNApol ELISA tests were 4% (21/175) and 12% (21/175), respectively. The ELISAs demonstrated values of 77.9% (95% posterior probability interval (PPI) = 52.5–95%) sensitivity and 87.7% (PPI = 82.5–91.9%) specificity, respectively. Accordingly, the sera obtained from the elephants exhibiting no clinical signs of EEHV infection, and those who were negative according to PCR tests, revealed a value of 14% seropositivity for EEHV-DNApol. Our results indicate that these asymptomatic, active EEHV-infected elephants could likely serve as a source of EEHV shedding within elephant herds. Consequently, the developed EEHV-DNApol NSPs-based ELISA test employed in the present study may be of use for routine monitoring and identification of EEHV shedders in elephant herds, and could be an alternative diagnostic tool for EEHV detection in Asian elephants.
Movement Patterns of Roaming Companion Cats in Denmark—A Study Based on GPS Tracking
Authors: Jensen Helene Ane , Meilby Henrik , Nielsen Søren Saxmose , Sandøe Peter
Published: July 2022

We studied the roaming patterns of companion cats in Denmark. The movements of 97 cats with outdoor access were traced for about seven days using GPS tracking. Data on the cats were gathered from their owners. The median time cats spent away from their homes was 5 h per day (IQR: 2.5 to 8.8 h), median daily distance moved was 2.4 km (IQR: 1.3 to 3.7 km), and median for 95% BBKDE home range was 5 ha (IQR: 2.9 to 8.5 ha). Cats above seven years of age spent less time away from home, were less active and had a smaller home range than younger cats. Cats with access to nature areas spent more time away from home, were more active and had larger home ranges. Intact male cats spent more time away from home than neutered cats and had larger home ranges as well. Finally, rainfall had an impact on the distance moved by cats: on days without rainfall the cats moved 3.6 km on average (95% CI: 2.8; 4.5 km); and on days with heavy rainfall the cats moved 2.4 km on average (95% CI: 1.6; 3.5 km).
Influence of Using Perforated Plastic Flooring Beneath the Waterline on Growth Performance, Litter Quality, and Footpad Health of Broiler Chickens: A Field Study
Authors: Sonnabend Sylvan-Justin , Spieß Fabian , Reckels Bernd , Ahmed Marwa F. E. , El-Wahab Amr Abd , Sürie Christian , Lingens Jan Berend , Visscher Christian
Published: July 2022

The aim of this study was to evaluate the effect of slatted flooring beneath waterlines in broiler barns on litter quality and, subsequently, footpad health. The hypothesis tested was that installing slatted flooring underneath waterlines helps to improve litter quality and thus reduces footpad diseases, enhancing animal welfare as a result. Five experimental runs with two groups were conducted. Each run was defined as one fattening period of 32 days and consisted of 15,000 broiler Ross 308 of both sexes. Every barn was divided into three areas (drinkers, feeders, and comfort area) for weekly sampling. No influence on growth performance was noted. The slatted flooring influenced the litter quality by preventing the litter in the experimental group (EG = 690 ± 167 g/kg DM) from becoming moisture until day 14 of the fattening period compared to the control group (CON = 636 ± 198 g/kg DM). The footpad health was also influenced by using slatted flooring, with lower camera-based footpad scores in the EG (8.80) compared to CON (22.0) at the slaughterhouse (p = 0.0258). Installing slatted flooring beneath the waterline reduced the moisture of the litter compared to the control barn in the first two weeks of age and showed a positive effect on the footpad health of the broilers at the end of fattening, which indicates an improvement in animal welfare.
Assessment of Indoor Air Quality for Group-Housed Macaques (Macaca spp.)
Authors: Maaskant Annemiek , Janssen Isabel , Wouters Inge M. , Eerdenburg Frank J. C. M. , Remarque Edmond J. , Langermans Jan A. M. , Bakker Jaco
Published: July 2022

Indoor Air Quality (IAQ) is strongly associated with animal health and wellbeing. To identify possible problems of the indoor environment of macaques (Macaca spp.), we assessed the IAQ. The temperature (°C), relative humidity (%) and concentrations of inhalable dust (mg/m3), endotoxins (EU/m3), ammonia (ppm) and fungal aerosols were measured at stationary fixed locations in indoor enclosures of group-housed rhesus (Macaca mulatta) and cynomolgus macaques (Macaca fascicularis). In addition, the personal exposure of caretakers to inhalable dust and endotoxins was measured and evaluated. Furthermore, the air circulation was assessed with non-toxic smoke, and the number of times the macaques sneezed was recorded. The indoor temperature and relative humidity for both species were within comfortable ranges. The geometric mean (GM) ammonia, dust and endotoxin concentrations were 1.84 and 0.58 ppm, 0.07 and 0.07 mg/m3, and 24.8 and 6.44 EU/m3 in the rhesus and cynomolgus macaque units, respectively. The GM dust concentrations were significantly higher during the daytime than during the nighttime. Airborne fungi ranged between 425 and 1877 CFU/m3. Personal measurements on the caretakers showed GM dust and endotoxin concentrations of 4.2 mg/m3 and 439.0 EU/m3, respectively. The number of sneezes and the IAQ parameters were not correlated. The smoke test revealed a suboptimal air flow pattern. Although the dust, endotoxins and ammonia were revealed to be within accepted human threshold limit values (TLV), caretakers were exposed to dust and endotoxin levels exceeding existing occupational reference values.
Effects of Different Concentrations of Lactobacillus plantarum and Bacillus licheniformis on Silage Quality, In Vitro Fermentation and Microbial Community of Hybrid Pennisetum
Authors: Zhu Yanchen , Xiong Haoming , Wen Zhiying , Tian Hanchen , Chen Yiye , Wu Longfei , Guo Yongqing , Sun Baoli
Published: July 2022

The purpose of the experiment was to study the effects of different concentrations of Lactobacillus plantarum (LP) and Bacillus licheniformis (BL) on the quality of hybrid Pennisetum (HP) silage. The experiment consisted of five treatment groups. The control group did not use additives, and the experimental groups were added with LP or BL of 1 × 105 cfu/g fresh weight (FW) and 1 × 107 cfu/g FW, respectively. The results showed that LP and BL could increase the in vitro fermentation gas production and reduce the ammonia nitrogen (AN) content in HP silage. Water-soluble carbohydrates (WSC), lactic acid (LA) content, and gas production in the LP group were positively correlated with LP addition, and acetic acid (AA) was negatively correlated with addition. The content of WSC and LA in the LP7 group was significantly higher than that in the control group (p < 0.05), and AA was lower than that in the control group (p > 0.05). Dry matter (DM), crude protein (CP), and gas production were negatively correlated with the addition of BL, while acid detergent fiber (ADF) content was positively correlated with the addition of BL. Furthermore, in the above indicators, the BL5 group reached a significant level with the control group (p < 0.05). The results of 16sRNA showed that the use of LP and BL could increase the relative abundance of Lactobacillus and decrease the relative abundance of Weissella in HP silage compared with the control group. In conclusion, LP and BL can significantly improve the quality of HP silage. The LP7 group and the BL5 group have the best silage effect. From the perspective of gas production in in vitro fermentation, the LP7 group had stronger fermentability and higher nutritional value.
Development of an Artificial Neural Network for the Detection of Supporting Hindlimb Lameness: A Pilot Study in Working Dogs
Authors: Figueirinhas Pedro , Sanchez Adrián , Rodríguez Oliver , Vilar José Manuel , Rodríguez-Altónaga José , Gonzalo-Orden José Manuel , Quesada Alexis
Published: July 2022

Subjective lameness assessment has been a controversial subject given the lack of agreement between observers; this has prompted the development of kinetic and kinematic devices in order to obtain an objective evaluation of locomotor system in dogs. After proper training, neural networks are potentially capable of making a non-human diagnosis of canine lameness. The purpose of this study was to investigate whether artificial neural networks could be used to determine canine hindlimb lameness by computational means only. The outcome of this study could potentially assess the efficacy of certain treatments against diseases that cause lameness. With this aim, input data were obtained from an inertial sensor positioned on the rump. Data from dogs with unilateral hindlimb lameness and sound dogs were used to obtain differences between both groups at walk. The artificial neural network, after necessary adjustments, was integrated into a web management tool, and the preliminary results discriminating between lame and sound dogs are promising. The analysis of spatial data with artificial neural networks was summarized and developed into a web app that has proven to be a useful tool to discriminate between sound and lame dogs. Additionally, this environment allows veterinary clinicians to adequately follow the treatment of lame canine patients.
Vitamin and Mineral Supplementation and Rate of Gain in Beef Heifers I: Effects on Dam Hormonal and Metabolic Status, Fetal Tissue and Organ Mass, and Concentration of Glucose and Fructose in Fetal Fluids at d 83 of Gestation
Authors: B. Menezes Ana Clara , McCarthy Kacie L. , Kassetas Cierrah J. , Baumgaertner Friederike , Kirsch James D. , Dorsam Sheri T. , Neville Tammi L. , Ward Alison K. , Borowicz Pawel P. , Reynolds Lawrence P. , Sedivec Kevin K. , Forcherio J. Chris , Scott Ronald , Caton Joel S. , Dahlen Carl R.
Published: July 2022

Thirty-five crossbred Angus heifers (initial BW = 359.5 ± 7.1 kg) were randomly assigned to a 2 × 2 factorial design to evaluate effects of vitamin and mineral supplementation [VMSUP; supplemented (VTM) vs. unsupplemented (NoVTM)] and different rates of gain [GAIN; low gain (LG), 0.28 kg/d, vs. moderate gain (MG), 0.79 kg/d] during the first 83 d of gestation on dam hormone and metabolic status, fetal tissue and organ mass, and concentration of glucose and fructose in fetal fluids. The VMSUP was initiated 71 to 148 d before artificial insemination (AI), allowing time for mineral status of heifers to be altered in advance of breeding. At AI heifers were assigned their GAIN treatment. Heifers received treatments until the time of ovariohysterectomy (d 83 ± 0.27 after AI). Throughout the experiment, serum samples were collected and analyzed for non-esterified fatty acids (NEFA), progesterone (P4), insulin, and insulin-like growth factor 1 (IGF-1). At ovariohysterectomy, gravid reproductive tracts were collected, measurements were taken, samples of allantoic (ALF) and amniotic (AMF) fluids were collected, and fetuses were dissected. By design, MG had greater ADG compared to LG (0.85 vs. 0.34 ± 0.04 kg/d, respectively; p < 0.01). Concentrations of NEFA were greater for LG than MG (p = 0.04) and were affected by a VMSUP × day interaction (p < 0.01), with greater concentrations for NoVTM on d 83. Insulin was greater for NoVTM than VTM (p = 0.01). A GAIN × day interaction (p < 0.01) was observed for IGF-1, with greater concentrations for MG on d 83. At d 83, P4 concentrations were greater for MG than LG (GAIN × day, p < 0.01), and MG had greater (p < 0.01) corpus luteum weights versus LG. Even though fetal BW was not affected (p ≥ 0.27), MG fetuses had heavier (p = 0.01) femurs than LG, and VTM fetuses had heavier (p = 0.05) livers than those from NoVTM. Additionally, fetal liver as a percentage of BW was greater in fetuses from VTM (P = 0.05; 3.96 ± 0.06% BW) than NoVTM (3.79 ± 0.06% BW), and from LG (p = 0.04; 3.96 ± 0.06% BW) than MG (3.78 ± 0.06% BW). A VMSUP × GAIN interaction was observed for fetal small intestinal weight (p = 0.03), with VTM-MG being heavier than VTM-LG. Therefore, replacement heifer nutrition during early gestation can alter the development of organs that are relevant for future offspring performance. These data imply that compensatory mechanisms are in place in the developing conceptus that can alter the growth rate of key metabolic organs possibly in an attempt to increase or decrease energy utilization.
The Potential Impact of a Dog Training Program on the Animal Adoptions in an Italian Shelter
Authors: d’Angelo Danila , Sacchettino Luigi , Quaranta Angelo , Visone Michele , Avallone Luigi , Gatta Claudia , Napolitano Francesco
Published: July 2022

One of the main concerns of the human–dog relationship is today associated with the quality life inside the kennels, which are very often regarded as animal dump where dogs are exiled, representing a burden on society. In the present study we sought to investigate the importance of performing an appropriate behavioral program on the adoption chances within an Italian shelter, near Naples (Ottaviano). In this respect, we enrolled 555 adopted dogs of different ages, who followed a tailored-4-month lasting training program between 2018 and 2020. Once entered there, they were carefully examined by the veterinary behaviorist, and directed towards a suited training program, to improve living conditions. We documented a higher number of both adult and senior dogs who left the kennel and were adopted, compared to the age-matched untrained animals (n = 479), housed in the same kennel from 2015 to 2017. Taken together, the present data highlight an important role for training in improving the natural attitudes of the companion dogs, thus pointing towards a better human–animal bond.
Selected Techniques for Physiotherapy in Dogs
Authors: Dybczyńska Marta , Goleman Małgorzata , Garbiec Aleksandra , Karpiński Mirosław
Published: July 2022

Physiotherapy is a new dynamically developing field of science in which the original idea was to improve the care for convalescent patients. Its positive effects observed in humans suggested the need for the adaptation and implementation of human physiotherapy techniques in animal care. Dogs are the second group of animals that undergo physiotherapy most frequently. These animals are diagnosed with a number of locomotor system problems, which may be congenital and are often related to the breed or acquired. The aim of the study was to collect and systematize knowledge of animal physiotherapy with emphasis on the selection and description of the most important aspects of canine physiotherapy. The review material consisted of 59 publications, with 230 selected for the review. Physiotherapeutic treatments are applied not only for rehabilitation of animals but also in healthy animals to upgrade their sports performance and improve their welfare. A majority of physiotherapeutic approaches have been transferred from human protocols. A key issue in the achievement of therapy success is the proper selection of physiotherapeutic procedures and close cooperation between the veterinarian and the physiotherapist.
3D Models as a Tool to Assess the Anti-Tumor Efficacy of Therapeutic Antibodies: Advantages and Limitations
Authors: Guzzeloni Virginia , Veschini Lorenzo , Pedica Federica , Ferrero Elisabetta , Ferrarini Marina
Published: July 2022

Therapeutic monoclonal antibodies (mAbs) are an emerging and very active frontier in clinical oncology, with hundred molecules currently in use or being tested. These treatments have already revolutionized clinical outcomes in both solid and hematological malignancies. However, identifying patients who are most likely to benefit from mAbs treatment is currently challenging and limiting the impact of such therapies. To overcome this issue, and to fulfill the expectations of mAbs therapies, it is urgently required to develop proper culture models capable of faithfully reproducing the interactions between tumor and its surrounding native microenvironment (TME). Three-dimensional (3D) models which allow the assessment of the impact of drugs on tumors within its TME in a patient-specific context are promising avenues to progressively fill the gap between conventional 2D cultures and animal models, substantially contributing to the achievement of personalized medicine. This review aims to give a brief overview of the currently available 3D models, together with their specific exploitation for therapeutic mAbs testing, underlying advantages and current limitations to a broader use in preclinical oncology.
Monitoring Carbapenem-Resistant Enterobacterales in the Environment to Assess the Spread in the Community
Authors: Urase Taro , Goto Saki , Sato Mio
Published: July 2022

The usefulness of wastewater-based epidemiology (WBE) was proven during the COVID-19 pandemic, and the role of environmental monitoring of emerging infectious diseases has been recognized. In this study, the prevalence of carbapenem-resistant Enterobacterales (CRE) in Japanese environmental samples was measured in the context of applying WBE to CRE. A total of 247 carbapenem-resistant isolates were obtained from wastewater, treated wastewater, and river water. Treated wastewater was shown to be an efficient target for monitoring CRE. The results of the isolate analysis showed that WBE may be applicable to Escherichia coli-carrying New Delhi metallo-β-lactamase (NDM)-type carbapenemase, the Enterobacter cloacae complex and Klebsiella pneumoniae complex-carrying IMP-type carbapenemase. In addition, a certain number of CRE isolated in this study carried Guiana extended spectrum (GES)-type carbapenemase although their clinical importance was unclear. Only a few isolates of Klebsiella aerogenes were obtained from environmental samples in spite of their frequent detection in clinical isolates. Neither the KPC-type, the oxacillinase (OXA)-type nor the VIM-type of carbapenemase was detected in the CRE, which reflected a low regional prevalence. These results indicated the expectation and the limitation of applying WBE to CRE.
Plants with Antimicrobial Activity Growing in Italy: A Pathogen-Driven Systematic Review for Green Veterinary Pharmacology Applications
Authors: Piras Cristian , Tilocca Bruno , Castagna Fabio , Roncada Paola , Britti Domenico , Palma Ernesto
Published: July 2022

Drug resistance threatening humans may be linked with antimicrobial and anthelmintic resistance in other species, especially among farm animals and, more in general, in the entire environment. From this perspective, Green Veterinary Pharmacology was proven successful for the control of parasites in small ruminants and for the control of other pests such as varroa in bee farming. As in anthelmintic resistance, antimicrobial resistance (AMR) represents one of the major challenges against the successful treatment of infectious diseases, and antimicrobials use in agriculture contributes to the spread of more AMR bacterial phenotypes, genes, and proteins. With this systematic review, we list Italian plants with documented antimicrobial activity against possible pathogenic microbes. Methods: The literature search included all the manuscripts published since 1990 in PubMed, Web of Science, and Scopus using the keywords (i) “antimicrobial, plants, Italy”; (ii) “antibacterial, plant, Italy”; (iii) “essential oil, antibacterial, Italy”; (iv) “essential oil, antimicrobial, Italy”; (v) “methanol extract, antibacterial, Italy”; (vi) “methanol extract, antimicrobial, Italy”. Results: In total, 105 manuscripts that documented the inhibitory effect of plants growing in Italy against bacteria were included. One hundred thirty-five plants were recorded as effective against Gram+ bacteria, and 88 against Gram−. This will provide a ready-to-use comprehensive tool to be further tested against the indicated list of pathogens and will suggest new alternative strategies against bacterial pathogens to be employed in Green Veterinary Pharmacology applications.
The Production of Antibiotics Must Be Reoriented: Repositioning Old Narrow-Spectrum Antibiotics, Developing New Microbiome-Sparing Antibiotics
Authors: Diamantis Sylvain , Retur Nicolas , Bertrand Benjamin , Lieutier-Colas Florence , Carenco Philippe , Mondain Véronique ,   on behalf of PROMISE Professional Community Network on Antimicrobial Resistance
Published: July 2022

The development of broad-spectrum antibiotics to control multidrug-resistant bacteria is an outdated business model. This strategy has led to the introduction of highly effective antibiotics, but their widespread use has contributed to the emergence of even broader antibiotic resistance. In a strategy to combat antimicrobial resistance, we believe that the use of narrow-spectrum antibiotics should be promoted. This should involve both the repositioning of old antibiotics and the reorientation of research and development towards new narrow-spectrum antibiotics with a low ecological impact. These antibiotics could be prescribed for common conditions such as sore throats and cystitis, which account for the bulk of antibiotic use in humans. Narrow-spectrum, targeted, microbiome-sparing antibiotics could help control antibiotic resistance while being economically sustainable. Their development and production should be supported by governments, which would ultimately benefit from reduced health care costs.
Path Planning of Multi-Type Robot Systems with Time Windows Based on Timed Colored Petri Nets
Authors: He Zhou , Zhang Ruijie , Ran Ning , Gu Chan
Published: July 2022

Mobile robots are extensively used to complete repetitive operations in industrial areas such as intelligent transportation, logistics, and manufacturing systems. This paper addresses the path planning problem of multi-type robot systems with time windows based on timed colored Petri nets. The tasks to be completed are divided into three different types: common, exclusive and collaborative. An analytical approach to plan a group of different types of mobile robots is developed to ensure that some specific robots will visit task regions within given time windows. First, a multi-type robot system and its environment are modeled by a timed colored Petri net. Then, some methods are developed to convert the task requirements that contain logic constraints and time windows into linear constraints. Based on integer linear programming techniques, a planning approach is proposed to minimize the total cost (i.e., total travel distance) of the system. Finally, simulation studies are investigated to show the effectiveness of the developed approach.
Actimetry-Derived 24 h Rest–Activity Rhythm Indices Applied to Predict MCTQ and PSQI
Authors: Borisenkov Mikhail , Tserne Tatyana , Bakutova Larisa , Gubin Denis
Published: July 2022

The aim of this study was to identify wrist actimetry-based indices associated with the sleep–wake rhythm characteristics of healthy individuals. The study involved 79 healthy subjects of both sexes (age range 15–62 years (mean: 21.5 ± 9.6 years, women: 77.8%)). Each participant provided personal data, filled out two questionnaires, the Munich ChronoType Questionnaire (MCTQ) and the Pittsburgh Sleep Quality Index (PSQI), and wore a wrist actimeter for a week. A significant positive association of the chronotype with the mid-phase of the most active 10 h period (M10t: B = 0.252, p = 0.015), the mid-phase of the least active 5 h period (L5t: B = 0.338, p = 0.005), and the interdaily stability (IS: B = −0.021, p = 0.017) was noted, as well as the sleep duration with the M10t (B = −0.257, p = 0.003), L5t (B = −0.340, p = 0.001), and IS (B = 0.042, p = 0.003). There was a significant association of social jetlag (B = 0.320, p = 0.032) and sleep quality (B = 0.990, p = 0.013) with motor activity in bed, as well as sleep efficiency with the acrophase (B = −0.043, p = 0.007). Nonparametric indices of the 24 h rest–activity rhythm are useful tools for assessing the sleep–wake rhythm of healthy individuals.
Augmented Reality in Surgery: A Scoping Review
Authors: Barcali Eleonora , Iadanza Ernesto , Manetti Leonardo , Francia Piergiorgio , Nardi Cosimo , Bocchi Leonardo
Published: July 2022

Augmented reality (AR) is an innovative system that enhances the real world by superimposing virtual objects on reality. The aim of this study was to analyze the application of AR in medicine and which of its technical solutions are the most used. We carried out a scoping review of the articles published between 2019 and February 2022. The initial search yielded a total of 2649 articles. After applying filters, removing duplicates and screening, we included 34 articles in our analysis. The analysis of the articles highlighted that AR has been traditionally and mainly used in orthopedics in addition to maxillofacial surgery and oncology. Regarding the display application in AR, the Microsoft HoloLens Optical Viewer is the most used method. Moreover, for the tracking and registration phases, the marker-based method with a rigid registration remains the most used system. Overall, the results of this study suggested that AR is an innovative technology with numerous advantages, finding applications in several new surgery domains. Considering the available data, it is not possible to clearly identify all the fields of application and the best technologies regarding AR.
Fabrication of Cylindrical Microlens Array on RB-SiC Moulds by Precision Grinding with MAWJ-Textured Diamond Wheels
Authors: Su Fukang , Zhang Zhenzhong , Yao Peng , Yu Hanwen , Xing Hongyu , Ge Mengran , Zhao Yanhua
Published: July 2022

Cylindrical microlens array (CMA) is applied widely in imaging, sensing, and laser machining fields. Among the many techniques for machining CMA, moulding is considered a mass-production method with low-cost and good accuracy. Aimed at the present problems in the machining of CMA moulds, which include low processing efficiency and the prediction of the surface topography, this paper focused on the fabrication of CMA on RB-SiC moulds by precision grinding with micro-abrasive water jet (MAWJ) textured diamond wheels. The combined rough–fine grinding strategy for ceramic mould materials was proposed. The grinding experiments of CMA were carried out. The ultra-precision grinding method was optimized to obtain high shape accuracy and a high-quality surface of RB-SiC moulds. It was found that by using MAWJ-textured diamond wheels, the profile error in the peak-to-valley value (PV) of the CMA moulds can be further reduced to 6.7 μm by using the combined rough–fine strategy grinding process.