bluuebunny/crossref_metadata_2025 huggingface dataset:
Mutations in the dwarf3 gene confer height stability in sorghum
Authors: Diatta‐Holgate Elisabeth , Bergsma Ben , Tuinstra Mitchell R.
Published: May 2024

Dwarfism is a useful trait in many crop plants because it contributes to improved lodging resistance and harvest index. The mutant allele dw3‐ref (dwarf3‐reference) of sorghum [Sorghum bicolor (L.) Moench] is characterized by an 882 bp tandem duplication in the fifth exon of the gene that is unstable and reverts to wild‐type at a frequency greater than 0.001 in many genetic backgrounds. The goal of this research was to identify stable alleles of dw3 (dwarf3) that could be backcrossed into elite parent lines to improve height stability of the crop. To discover new alleles of dw3, a panel consisting mostly of sorghum conversion lines (SC‐lines) was screened by polymerase chain reaction for the 882 bp tandem duplication in the fifth exon of dw3‐ref. Sanger sequencing was used to characterize the DNA sequence of this fragment in genotypes that did not contain the 882 bp tandem duplication. Sequence analysis identified three indel mutations, including an 82 bp deletion, a 6 bp duplication, and a 15 bp deletion in this region of the gene. Field trials of the donor genotypes with these new alleles indicated no wild‐type revertants of dw3‐sd3 (dwarf3‐stable dwarf), dw3‐sd4, and dw3‐sd5. These alleles were backcrossed into Tx430. Field trials of backcross progeny (BC2F4) with the dw3‐sd3, dw3‐sd4, and dw3‐sd5 alleles indicated no revertants. The plant height and flowering time characteristics of the backcross progeny were similar or slightly shorter and earlier than the recurrent parent. These findings demonstrate that dw3‐sd3, dw3‐sd4, and dw3‐sd5 alleles will be useful in breeding for the stable dwarf trait.
Visualization of caudothalamic groove at expert fetal neurosonography
Authors: di Pasquo E. , Contro E. , Labadini C. , Dall'Asta A. , Volpe N. , Larcher L. , Vettor L. , Piemonti L. , Ormitti F. , Ghi T.
Published: May 2024

Objectives

To describe the sonographic features of the caudothalamic groove in the third trimester of pregnancy in a group of structurally normal fetuses and to report a small series of cases with abnormal appearance of the caudothalamic groove at antenatal cranial ultrasound.

Methods

This was an observational study conducted at two fetal medicine referral units in Italy. A non‐consecutive cohort of pregnant women with a singleton non‐anomalous pregnancy were recruited prospectively and underwent three‐dimensional (3D) ultrasound assessment of the fetal brain at 28–32 weeks' gestation. At offline analysis, the ultrasound volumes were adjusted in the multiplanar mode, according to a standardized methodology, until the caudothalamic groove was visible in the parasagittal plane. To evaluate interobserver agreement, two operators were asked independently to indicate if the caudothalamic groove was visible unilaterally or bilaterally on each volume and Cohen's kappa (κ) coefficient was calculated. The digital archives of the two centers were also searched retrospectively to retrieve cases with abnormal findings at the level of the caudothalamic groove on antenatal cranial ultrasound that were confirmed postnatally.

Results

A total of 180 non‐consecutive cases were included. At offline analysis of the 3D ultrasound volumes, the caudothalamic groove was identified in the parasagittal plane by both operators at least unilaterally in 176 (97.8%) cases and bilaterally in 174 (96.7%) cases. The κ‐coefficient for the agreement between the two independent operators in recognizing the caudothalamic groove was 0.89 and 0.83 for one and both hemispheres, respectively. The retrospective search of our archives yielded five cases with an abnormal appearance of the caudothalamic groove at antenatal cranial ultrasound, including two cases of hemorrhage and three cases of cyst.

Conclusions

The caudothalamic groove is consistently seen in normal fetuses on multiplanar neurosonography in the third trimester, and abnormal findings in this region may be detected antenatally. © 2024 The Authors. Ultrasound in Obstetrics & Gynecology published by John Wiley & Sons Ltd on behalf of International Society of Ultrasound in Obstetrics and Gynecology.
Jet charge identification in the e+e− → Z → $$ \textrm{q}\overline{\textrm{q}} $$ process at Z pole
Authors: Cui Hanhua , Zhao Mingrui , Wang Yuexin , Liang Hao , Ruan Manqi
Published: May 2024

Abstract

Accurate jet charge identification is essential for precise electroweak and flavor measurements at the high-energy frontier. We propose a novel method called the Leading Particle Jet Charge method (LPJC) to determine the jet charge based on information of the leading charged particle. Tested on Z →$$ b\overline{b} $$
b

b
¯

and Z →$$ c\overline{c} $$
c

c
¯

samples at center-of-mass energy of 91.2 GeV, the LPJC achieves an effective tagging power ϵeff of 20%/9% for c/b jet, respectively. In combination with the Weighted Jet Charge method (WJC), we develop a Heavy Flavor Jet Charge method (HFJC), which achieves an effective tagging power ϵeff of 39%/20% for c/b jet, respectively. This paper also discusses the dependencies between jet charge identification performance and the fragmentation process of heavy flavor jets, as well as critical detector performances.
Positivity from J-Basis operators in the standard model effective Field Theory
Authors: Yang Chengjie , Ren Zhe , Yu Jiang-Hao
Published: May 2024

Abstract

In the effective field theory (EFT), the positivity bound on dim-8 effective operators tells us that the s2 contribution in the scattering amplitude of 2-to-2 process geometrically corresponds to the convex cone composed of the ultraviolet (UV) states as the extremal rays. The J-Basis method can provide a complete group theory decomposition of the scattering amplitude on the direct product of the gauge group and the Lorentz group, thus to search for all UV states. Compared to previous methods, which can only perform direct product decomposition on the gauge groups, the J-Basis method greatly improves the strictness of the restrictions and also provides a systematic scheme for calculating the positivity bounds of the dim-8 operators.
A case study of SMEFT $$ \mathcal{O}\left(1/{\Lambda}^4\right) $$ effects in diboson processes: pp → W±(ℓ±ν)γ
Authors: Martin Adam
Published: May 2024

Abstract

In this paper we explore pp → W±(ℓ±ν)γ to $$ \mathcal{O}\left(1/{\Lambda}^4\right) $$
O

1
/

Λ
4

in the SMEFT expansion. Calculations to this order are necessary to properly capture SMEFT contributions that grow with energy, as the interference between energy-enhanced SMEFT effects at $$ \mathcal{O}\left(1/{\Lambda}^2\right) $$
O

1
/

Λ
2

and the Standard Model is suppressed. We find that there are several dimension eight operators that interfere with the Standard Model and lead to the same energy growth, ~ $$ \mathcal{O}\left({E}^4/{\Lambda}^4\right) $$
O

E
4

/

Λ
4

, as dimension six squared. While energy-enhanced SMEFT contributions are a main focus, our calculation includes the complete set of $$ \mathcal{O}\left(1/{\Lambda}^4\right) $$
O

1
/

Λ
4

SMEFT effects consistent with U(3)5 flavor symmetry. Additionally, we include the decay of the W±→ ℓ±ν, making the calculation actually $$ \overline{q}{q}^{\prime}\to {\ell}^{\pm}\nu \gamma $$

q
¯

q
′

→

ℓ
±

νγ
. As such, we are able to study the impact of non-resonant SMEFT operators, such as $$ \left({L}^{\dagger }{\overline{\sigma}}^{\mu }{\tau}^IL\right)\left({Q}^{\dagger }{\overline{\sigma}}^{\nu }{\tau}^IQ\right) $$

L
†

σ
¯

μ

τ
I

L

Q
†

σ
¯

ν

τ
I

Q

Bμν, which contribute to $$ \overline{q}{q}^{\prime}\to {\ell}^{\pm}\nu \gamma $$

q
¯

q
′

→

ℓ
±

νγ
directly and not to $$ \overline{q}{q}^{\prime}\to {W}^{\pm}\gamma $$

q
¯

q
′

→

W
±

γ
. We show several distributions to illustrate the shape differences of the different contributions.
High-Energy Behavior of Scattering Amplitudes in Theories with Purely Virtual Particles
Authors: Piva Marco
Published: May 2024

Abstract

We study a class of renormalizable quantum field theories with purely virtual particles that exhibits nonrenormalizable behavior in the high-energy limit of scattering cross sections, which grow as powers of the center-of-mass energy squared and seems to violate unitarity bounds. We point out that the problem should be viewed as a violation of perturbativity, instead of unitarity, and show that the resummation of self energies fixes the issue. As an explicit example, we consider a class of O(N) theories at the leading order in the large-N expansion and show that the different quantization prescription of purely virtual particles takes care of the nonrenormalizable behavior, making the resummed cross sections to decrease at high energies and the amplitudes to satisfy the unitarity bounds. We compare the results to the case of theories with ghosts, where the resummation cannot change the behavior of cross sections due to certain cancellations in the high-energy expansion of the self energies. These results are particularly relevant for quantum gravity.
Eduction of coherent structures from schlieren images of twin jets using SPOD informed with momentum potential theory in the spectral domain
Authors: Padilla-Montero Iván , Rodríguez Daniel , Jaunet Vincent , Jordan Peter
Published: May 2024

This work presents a methodology to extract coherent structures from high-speed schlieren images of turbulent twin jets which are more physically interpretable than those obtained with currently existing techniques. Recently, Prasad and Gaitonde (J Fluid Mech 940:1–11, 2022) introduced an approach which employs the momentum potential theory of Doak (J Sound Vib 131(1):67–90, 1989) to compute potential (acoustic and thermal) energy fluctuations from the schlieren images by solving a Poisson equation, and combines it with spectral proper orthogonal decomposition (SPOD) to educe coherent structures from the momentum potential field instead of the original schlieren field. While the latter field is dominated by a broad range of vortical fluctuations in the turbulent mixing region of unheated high-speed jets, the momentum potential field is governed by fluctuations which are intimately related to acoustic emission, and its spatial structure in the frequency domain is very organized. The proposed methodology in this paper improves the technique of Prasad and Gaitonde (J Fluid Mech 940:1–11, 2022) in three new ways. First, the solution of the Poisson equation is carried out in the frequency-wavenumber domain instead of the time-space domain, which simplifies and integrates the solution of the Poisson equation within the SPOD framework based on momentum potential fluctuations. Second, the issue of solving the Poisson equation on a finite domain with ad hoc boundary conditions is explicitly addressed, identifying and removing those unphysical harmonic components introduced in the solution process. Third, the solution of the SPOD problem in terms of momentum potential fluctuations is used to reconstruct schlieren SPOD fields associated with each mode, allowing the visualization of the obtained coherent structures also in terms of the density gradient. The method is applied here to schlieren images of a twin-jet configuration with a small jet separation at two supersonic operation conditions: a perfectly-expanded and an overexpanded one. The SPOD modes based on momentum potential fluctuations retain the wavepacket structure including the direct Mach-wave radiation, together with upstream- and downstream-traveling acoustic waves, similar to SPOD modes based on the schlieren images. However, for the same dataset, they result in a lower-rank decomposition than schlieren-based SPOD and provide an effective separation of twin-jet fluctuations into independent toroidal and flapping oscillations that are recovered as different SPOD modes. These coherent structures are more consistent with twin-jet wavepacket models available in the literature than those originally obtained with direct schlieren-based SPOD, facilitating their interpretation and comparison against theoretical analyses.

Graphical abstract
Variational Worn Stones
Authors: Crasta Graziano , Fragalà Ilaria
Published: May 2024

We introduce an evolution model à la Firey for a convex stone which tumbles on a beach and undertakes an erosion process depending on some variational energy, such as torsional rigidity, a principal Dirichlet Laplacian eigenvalue, or Newtonian capacity. Relying on the assumption of the existence of a solution to the corresponding parabolic flow, we prove that the stone tends to become asymptotically spherical. Indeed, we identify an ultimate shape of these flows with a smooth convex body whose ground state satisfies an additional boundary condition, and we prove symmetry results for the corresponding overdetermined elliptic problems. Moreover, we extend the analysis to arbitrary convex bodies: we introduce new notions of cone variational measures and we prove that, if such a measure is absolutely continuous with constant density, the underlying body is a ball.
Influence of the rehydration period on yield quality and harvest performance in Manzanilla de Sevilla super high-density olive orchards
Authors: Morales-Sillero Ana , González-Fernández Antonio , Casanova Laura , Martín-Palomo María José , Jiménez M. R. , Rallo Pilar , Moriana Alfonso
Published: May 2024

Super-high density olive orchards may increase profitability for table olive producers. However, water needs and fruit damage could limit their viability. Deficit irrigation scheduling would reduce the amount of water required, but rehydration before harvest is extremely important. The aim of this work was to compare a typical deficit farm management model with a regulated deficit irrigation one based on the plant water status. The experiment was carried out during three seasons in a three years-old (4 × 1.5 m) commercial table olive orchard. In both irrigation scheduling plots, seasonal amount of applied water was similar. Irrigation treatments were: common farm management (CFM), an almost constant rate of irrigation, and Regulated deficit (RDI) based on midday shaded water potential. Vegetative measurements suggested that RDI increased the external surface of the hedge, and this was related with greater yield in this treatment. Significant differences were found in of fruit size at harvest in some seasons, with larger fruits in RDI compared to CFM. However, fruit damage occurred during mechanical harvest in both irrigation managements, despite the higher skin firmness in RDI, and shows the need to carry out postharvest treatments to improve the quality of the final product. Accurate control of the tree water status would allow optimizing the amount of water available for table olive orchards.
Validation of the standardization framework SSTR-RADS 1.0 for neuroendocrine tumors using the novel SSTR‑targeting peptide [18F]SiTATE
Authors: Ebner R. , Lohse A. , Fabritius M. P. , Rübenthaler J. , Wängler C. , Wängler B. , Schirrmacher R. , Völter F. , Schmid H. P. , Unterrainer L. M. , Öcal O. , Hinterberger A. , Spitzweg C. , Auernhammer C. J. , Geyer T. , Ricke J. , Bartenstein P. , Holzgreve A. , Grawe F.
Published: May 2024

Objectives

Somatostatin receptor positron emission tomography/computed tomography (SSTR-PET/CT) using [68Ga]-labeled tracers is a widely used imaging modality for neuroendocrine tumors (NET). Recently, [18F]SiTATE, a SiFAlin tagged [Tyr3]-octreotate (TATE) PET tracer, has shown great potential due to favorable clinical characteristics. We aimed to evaluate the reproducibility of Somatostatin Receptor-Reporting and Data System 1.0 (SSTR-RADS 1.0) for structured interpretation and treatment planning of NET using [18F]SiTATE.

Methods

Four readers assessed [18F]SiTATE-PET/CT of 95 patients according to the SSTR-RADS 1.0 criteria at two different time points. Each reader evaluated up to five target lesions per scan. The overall scan score and the decision on peptide receptor radionuclide therapy (PRRT) were considered. Inter- and intra-reader agreement was determined using the intraclass correlation coefficient (ICC).

Results

The ICC analysis on the inter-reader agreement using SSTR-RADS 1.0 for identical target lesions (ICC ≥ 85%), overall scan score (ICC ≥ 90%), and the decision to recommend PRRT (ICC ≥ 85%) showed excellent agreement. However, significant differences were observed in recommending PRRT among experienced readers (ER) (p = 0.020) and inexperienced readers (IR) (p = 0.004). Compartment-based analysis demonstrated good to excellent inter-reader agreement for most organs (ICC ≥ 74%), except for lymph nodes (ICC ≥ 53%).

Conclusion

SSTR-RADS 1.0 represents a highly reproducible and consistent framework system for stratifying SSTR-targeted PET/CT scans, even using the novel SSTR-ligand [18F]SiTATE. Some inter-reader variability was observed regarding the evaluation of uptake intensity prior to PRRT as well as compartment scoring of lymph nodes, indicating that those categories require special attention during further clinical validation and might be refined in a future SSTR-RADS version 1.1.

Clinical relevance statement

SSTR-RADS 1.0 is a consistent framework for categorizing somatostatin receptor-targeted PET/CT scans when using [18F]SiTATE. The framework serves as a valuable tool for facilitating and improving the management of patients with NET.

Key Points

SSTR-RADS 1.0 is a valuable tool for managing patients with NET.

SSTR-RADS 1.0 categorizes patients with showing strong agreement across diverse reader expertise.

As an alternative to [68Ga]-labeled PET/CT in neuroendocrine tumor imaging, SSTR-RADS 1.0 reliably classifies [18F]SiTATE-PET/CT.
Correlation Between In Vitro and In Vivo Gene-Expression Strengths is Dependent on Bottleneck Process
Authors: Enomoto Toshihiko , Ohtake Kazumasa , Senda Naoko , Kiga Daisuke
Published: May 2024

Constructing gene networks in cells enables the efficient production of valuable substances and the creation of cells performing intended functions. However, the construction of a cellular network of interest, based on a design-build-test-learn cycle, is quite time-consuming due to processes mainly attributed to cell growth. Among the various available methods, cell-free systems have recently been employed for solving network testing problems using cells, because cell-free systems allow quick evaluations of test networks without waiting for cell growth. Although cell-free systems have the potential for use in rapid prototyping platforms, the correlation between the in vitro and in vivo activities for each genetic part (e.g. promoter) remains enigmatic. By quantifying mRNA and its encoded protein in a cell, we have identified appropriate culture conditions where cellular bottlenecks are circumvented and promoter activities are correlated with previous in vitro studies. This work provides a foundation for the development of molecular breadboard research.
A mixture distribution for modelling bivariate ordinal data
Authors: Ip Ryan H. L. , Wu K. Y. K.
Published: May 2024

Ordinal responses often arise from surveys which require respondents to rate items on a Likert scale. Since most surveys contain more than one question, the data collected are multivariate in nature, and the associations between different survey items are usually of considerable interest. In this paper, we focus on a mixture distribution, called the combination of uniform and binomial (CUB), under which each response is assumed to originate from either the respondent’s uncertainty or the actual feeling towards the survey item. We extend the CUB model to the bivariate case for modelling two correlated ordinal data without using copula-based approaches. The proposed model allows the associations between the unobserved uncertainty and feeling components of the variables to be estimated, a distinctive feature compared to previous attempts. This article describes the underlying logic and deals with both theoretical and practical aspects of the proposed model. In particular, we will show that the model is identifiable under a wide range of conditions. Practical inferential aspects such as parameter estimation, standard error calculations and hypothesis tests will be discussed through simulations and a real case study.
Estimation of population affinity using proximal femoral measurements based on computed tomographic images in the Japanese and western Australian populations
Authors: Torimitsu Suguru , Nakazawa Akari , Flavel Ambika , Swift Lauren , Makino Yohsuke , Iwase Hirotaro , Franklin Daniel
Published: May 2024

The present study analyzes morphological differences femora of contemporary Japanese and Western Australian individuals and investigates the feasibility of population affinity estimation based on computed tomographic (CT) data. The latter is deemed to be of practical importance because most anthropological methods rely on the assessment of aspects of skull morphology, which when damaged and/or unavailable, often hampers attempts to estimate population affinity. The study sample comprised CT scans of 297 (146 females; 151 males) Japanese and 330 (145 females; 185 males) Western Australian adult individuals. A total of 10 measurements were acquired in two-dimensional CT images of the left and right femora; two machine learning methods (random forest modeling [RFM]) and support vector machine [SVM]) were then applied for population affinity classification. The accuracy of the two-way (sex-specific and sex-mixed) model was between 71.38 and 82.07% and 76.09–86.09% for RFM and SVM, respectively. Sex-specific (female and male) models were slightly more accurate compared to the sex-mixed models; there were no considerable differences in the correct classification rates between the female- and male-specific models. All the classification accuracies were higher in the Western Australian population, except for the male model using SVM. The four-way sex and population affinity model had an overall classification accuracy of 74.96% and 79.11% for RFM and SVM, respectively. The Western Australian females had the lowest correct classification rate followed by the Japanese males. Our data indicate that femoral measurements may be particularly useful for classification of Japanese and Western Australian individuals.
A computational neural model that incorporates both intrinsic dynamics and sensory feedback in the Aplysia feeding network
Authors: Li Yanjun , Webster-Wood Victoria A. , Gill Jeffrey P. , Sutton Gregory P. , Chiel Hillel J. , Quinn Roger D.
Published: May 2024

Studying the nervous system underlying animal motor control can shed light on how animals can adapt flexibly to a changing environment. We focus on the neural basis of feeding control in Aplysia californica. Using the Synthetic Nervous System framework, we developed a model of Aplysia feeding neural circuitry that balances neurophysiological plausibility and computational complexity. The circuitry includes neurons, synapses, and feedback pathways identified in existing literature. We organized the neurons into three layers and five subnetworks according to their functional roles. Simulation results demonstrate that the circuitry model can capture the intrinsic dynamics at neuronal and network levels. When combined with a simplified peripheral biomechanical model, it is sufficient to mediate three animal-like feeding behaviors (biting, swallowing, and rejection). The kinematic, dynamic, and neural responses of the model also share similar features with animal data. These results emphasize the functional roles of sensory feedback during feeding.
Genetic determinants of renal scarring in children with febrile UTI
Authors: Rosenblad Therese , Lindén Magnus , Ambite Ines , Brandström Per , Hansson Sverker , Godaly Gabriela
Published: May 2024

Background

Febrile urinary tract infections (UTIs) are among the most severe bacterial infections in infants, in which a subset of patients develops complications. Identifying infants at risk of recurrent infections or kidney damage based on clinical signs is challenging. Previous observations suggest that genetic factors influence UTI outcomes and could serve as predictors of disease severity. In this study, we conducted a nationwide survey of infant genotypes to develop a strategy for infection management based on individual genetic risk. Our aims were to identify genetic susceptibility variants for renal scarring (RS) and genetic host factors predisposing to dilating vesicoureteral reflux (VUR) and recurrent UTIs.

Methods

To assess genetic susceptibility, we collected and analyzed DNA from blood using exome genotyping. Disease-associated genetic variants were identified through bioinformatics analysis, including allelic frequency tests and odds ratio calculations. Kidney involvement was defined using dimercaptosuccinic acid (DMSA) scintigraphy.

Results

In this investigation, a cohort comprising 1087 infants presenting with their first episode of febrile UTI was included. Among this cohort, a subset of 137 infants who underwent DMSA scanning was subjected to gene association analysis. Remarkable genetic distinctions were observed between patients with RS and those exhibiting resolved kidney involvement. Notably, the genetic signature indicative of renal scarring prominently featured mitochondrial genes.

Conclusions

In this nationwide study of genetic susceptibility to RS after febrile UTIs in infancy, we identified a profile dominated by mitochondrial polymorphisms. This profile can serve as a predictor of future complications, including RS and recurrent UTIs.

Graphical abstract
Systematic review and meta-analysis: relative age in attention-deficit/ hyperactivity disorder and autism spectrum disorder
Authors: Frisira Eleni , Holland Josephine , Sayal Kapil
Published: May 2024

Youngest students in their class, with birthdates just before the school entry cut-off date, are overrepresented among children receiving an Attention-Deficit/Hyperactivity Disorder (ADHD) diagnosis or medication for this. This is known as the relative age effect. This systematic review and meta-analysis summarises the evidence on the influence of relative age on ADHD symptoms, diagnosis and medication prescribing. As no review to date has investigated the association with autism spectrum disorder (ASD) diagnosis, this is also examined. Following prospective registration with PROSPERO, we conducted a systematic review according to the PRISMA guidelines. We searched seven databases: Medline, Embase, PsycInfo, Web of Science Core Collection, ERIC, Psychology and Behavioural Sciences Collection, and Cochrane Library. Additional references were identified from manual search of retrieved reviews. We performed a meta-analysis of quantitative data. Thirty-two studies were included, thirty-one investigated ADHD and two ASD. Younger relative age was associated with ADHD diagnosis and medication, with relative risks of 1.38 (1.36–1.52 95% CI) and 1.28 (1.21–1.36 95% CI) respectively. However, risk estimates exhibited high heterogeneity. A relative age effect was observed for teacher ratings of ADHD symptoms but not for parent ratings. With regard to ASD, the youngest children in their school year were more likely to be diagnosed with ASD. This review confirms a relative age effect for ADHD diagnosis and prescribed ADHD medication and suggests that differences in teacher and parent ratings might contribute to this. Further research is needed on the possible association with ASD.
Difference in coagulation systems of large animal species used in cardiovascular research: a systematic review
Authors: Staelens Louis , Langenaeken Tom , Rega Filip , Meuris Bart
Published: May 2024

Preclinical testing using animal models is indispensable in cardiovascular research. However, the translation to clinical practice of these animal models is questionable since it is not always clear how representative they are. This systematic review intends to summarize the interspecies differences in the coagulation profile of animal models used in cardiovascular research. It aims to guide future research in choosing the optimal animal species. A literature search of PubMed, Embase, Web of Science (Core Collection) and Cochrane Library was performed using a search string that was well defined and not modified during the study. An overview of the search terms used in each database can be found in the appendix. Articles describing coagulation systems in large animals were included. We identified 30 eligible studies of which 15 were included. Compared to humans, sheep demonstrated a less active external pathway of coagulation. Sheep had a higher platelet count but the platelet activatability and response to biomaterials were lower. Both sheep and pigs displayed no big differences in the internal coagulation system compared to humans. Pigs showed results very similar to those of humans, with the exception of a higher platelet count and stronger platelet aggregation in pigs. Coagulation profiles of different species used for preclinical testing show strong variation. Adequate knowledge of these differences is key in the selection of the appropriate species for preclinical cardiovascular research. Future thrombogenicity research should compare sheep to pig in an identical experimental setup.
Ellsberg 1961: text, context, influence
Authors: Moscati Ivan
Published: May 2024

In 1961 Daniel Ellsberg published an article titled “Risk, Ambiguity, and the Savage Axioms” in the Quarterly Journal of Economics, which became a seminal contribution to the theory of decision-making under uncertainty. This paper analyzes Ellsberg’s 1961 classic, situates it within the context of decision-making theory in the 1950s and early 1960s and within the development of Ellsberg’s ideas, and provides an overview of the experimental and theoretical literature to which it gave rise.
Experimental and numerical investigation of shelf flow crossing over a strait
Authors: Kuehl Joseph , Sheremet Vitalii A.
Published: May 2024

Motivated by the phenomenon of Scotian Shelf Crossover events, the problem of a shelf flow that is interrupted by a strait is considered. Laboratory experiments in a rotating tank with barotropic and baroclinic flow over flat and sloping shelves confirm that the flow is steered by the bathymetric contours and mainly circumnavigates the gulf. In order to jump across the strait, as suggested by earlier theories, the flow must have unrealistically high Rossby numbers. However, the near bottom friction relaxes the bathymetric constraint and causes the formation of a peculiar jet crossing the strait diagonally. For the dissipation values such that a half of the transport goes around the gulf and half crosses the strait diagonally, the diagonal crossover jet becomes most evident. Numerical solutions for realistic values of the frictional parameter reproduce the results of the laboratory experiments and consideration of the actual Gulf of Maine bathymetry reproduces patterns similar to those observed by drift trajectories and in the satellite derived sea surface temperature fields.
Incorporating pathological gait into patient-specific finite element models of the haemophilic ankle
Authors: Talbott Harriet G. , Wilkins Richard A. , Brockett Claire L. , Mengoni Marlène
Published: May 2024

Haemarthrosis is an inherent clinical feature of haemophilia, a disease characterised by an absence or reduction in clotting proteins. Patients with severe haemophilia experience joint bleeding leading to blood-induced ankle arthropathy (haemarthropathy). Altered biomechanics of the ankle have been reported in people with haemophilia; however, the consequence of this on joint health is little understood. The aim of this study was to assess the changes in joint contact due to haemophilia disease-specific gait features using patient-specific modelling, to better understand the link between biomechanics and joint outcomes. Four, image-based, finite element models of haemophilic ankles were simulated through consecutive events in the stance phase of gait, using both patient-specific and healthy control group (n = 36) biomechanical inputs. One healthy control FE model was simulated through the healthy control stance phase of the gait cycle for a point of comparison. The method developed allowed cartilage contact mechanics to be assessed throughout the loading phase of the gait cycle. This showed areas of increased contact pressure in the medial and lateral regions of the talar dome, which may be linked to collapse in these regions. This method may allow the relationship between structure and function in the tibiotalar joint to be better understood.
Majority opinion diffusion: when tie-breaking rule matters
Authors: Zehmakan Ahad N.
Published: May 2024

Consider a graph G, which represents a social network, and assume that initially each node is either blue or white (corresponding to its opinion on a certain topic). In each round, all nodes simultaneously update their color to the most frequent color in their neighborhood. This is called the Majority Model (MM) if a node keeps its color in case of a tie and the Random Majority Model (RMM) if it chooses blue with probability 1/2 and white otherwise. We study the convergence properties of the above models, including stabilization time, periodicity, and the number of stable configurations. In particular, we prove that the stabilization time in RMM can be exponential in the size of the graph, which is in contrast with the previously known polynomial bound on the stabilization time of MM. We provide some bounds on the minimum size of a winning set, which is a set of nodes whose agreement on a color in the initial coloring enforces the process to end in a coloring where all nodes share that color. Furthermore, we calculate the expected final number of blue nodes for a random initial coloring, where each node is colored blue independently with some fixed probability, on cycle graphs. Finally, we conduct some experiments which complement our theoretical findings and also let us investigate other aspects of the models.
Intraoperative spectroscopic evaluation of sentinel lymph nodes in breast cancer surgery
Authors: Barkur Surekha , Boitor Radu A. , Mihai Raluca , Gopal Navarasi S. Raja , Leeney Samuel , Koloydenko Alexey A. , Khout Hazem , Rakha Emad , Notingher Ioan
Published: May 2024

Background and objectives

Sentinel lymph node (SLN) biopsy is a standard procedure for patients with breast cancer and normal axilla on imaging. Positive SLNs on histological examination can lead to a subsequent surgery for axillary lymph node clearance (ALNC). Here we report a non-destructive technique based on autofluorescence (AF) imaging and Raman spectroscopy for intra-operative assessment of SLNs excised in breast cancer surgery.

Methods

A microscope integrating AF imaging and Raman spectroscopy modules was built to allow scanning of lymph node biopsy samples. During AF-Raman measurements, AF imaging determined optimal sampling locations for Raman spectroscopy measurements. After optimisation of the AF image analysis and training of classification models based on data from 85 samples, the AF-Raman technique was tested on an independent set of 81 lymph nodes comprising 58 fixed and 23 fresh specimens. The sensitivity and specificity of AF-Raman were calculated using post-operative histology as a standard of reference.

Results

The independent test set contained 66 negative lymph nodes and 15 positive lymph nodes according to the reference standard, collected from 78 patients. For this set of specimens, the area under the receiver operating characteristic (ROC) curve for the AF-Raman technique was 0.93 [0.83–0.98]. AF-Raman was then operated in a regime that maximised detection specificity, producing a 94% detection accuracy: 80% sensitivity and 97% specificity. The main confounders for SLN metastasis were areas rich in histiocytes clusters, for which only few Raman spectra had been included in the training dataset.

Discussion

This preliminary study indicates that with further development and extension of the training dataset by inclusion of additional Raman spectra of histiocytes clusters and capsule, the AF-Raman may become a promising technique for intra-operative assessment of SLNs. Intra-operative detection of positive biopsies could avoid second surgery for axillary clearance.
Impact of environmental temperature on the survival outcomes of breast cancer: A SEER-based study
Authors: Gupta Ashish , Roy Arya Mariam , Gupta Kush , Attwood Kristopher , Gandhi Asha , Edge Stephen , Takabe Kazuaki , Repasky Elizabeth , Yao Song , Gandhi Shipra
Published: May 2024

Background

Experimental evidence in tumor-bearing mouse models shows that exposure to cool, that is, sub-thermoneutral environmental temperature is associated with a higher tumor growth rate and an immunosuppressive tumor immune microenvironment than seen at thermoneutral temperatures. However, the translational significance of these findings in humans is unclear. We hypothesized that breast cancer patients living in warmer climates will have better survival outcomes than patients living in colder climates.

Methods

A retrospective population-based analysis was conducted on 270,496 stage I-III breast cancer patients, who were retrieved from the Surveillance, Epidemiology and End Results (SEER) over the period from 1996 to 2017. The average annual temperature (AAT) was calculated based on city level data from the National Centers for Environmental Information.

Results

A total of 270, 496 patients were analyzed. Temperature as assessed in quartiles. After adjusting for potential confounders, patients who lived in the 3rd and 4th quartile temperature regions with AAT 56.7–62.5°F (3rd quartile) and > 62.5°F (4th quartile) had a 7% increase in the OS compared to patients living at AAT < 48.5°F (1st quartile) (HR 0.93, 95% CI 0.90–0.95 and HR 0.93, 95% CI 0.91–0.96, respectively). For DSS, When comparing AAT quartiles, patients living with AAT in the range of 56.7–62.5°F and > 62.5°F demonstrated a 7% increase each in DSS after adjustment (HR 0.93, 95% CI 0.90–0.96 and HR 0.93, 95% CI 0.90–0.96).

Conclusions

Higher environmental temperatures are associated with significantly better OS and DSS in breast cancer patients. Future research is warranted to confirm this observation using large datasets to elucidate the underlying mechanisms and investigate novel therapeutic strategies to minimize this geographic disparity in clinical outcomes.
Exosomes enriched by miR-429-3p derived from ITGB1 modified Telocytes alleviates hypoxia-induced pulmonary arterial hypertension through regulating Rac1 expression
Authors: Qi Ruixue , Zhang Yong , Yan Furong
Published: May 2024

Background

Recent studies have emphasized the critical role of Telocytes (TCs)-derived exosomes in organ tissue injury and repair. Our previous research showed a significant increase in ITGB1 within TCs. Pulmonary Arterial Hypertension (PAH) is marked by a loss of microvessel regeneration and progressive vascular remodeling. This study aims to investigate whether exosomes derived from ITGB1-modified TCs (ITGB1-Exo) could mitigate PAH.

Methods

We analyzed differentially expressed microRNAs (DEmiRs) in TCs using Affymetrix Genechip miRNA 4.0 arrays. Exosomes isolated from TC culture supernatants were verified through transmission electron microscopy and Nanoparticle Tracking Analysis. The impact of miR-429-3p-enriched exosomes (Exo-ITGB1) on hypoxia-induced pulmonary arterial smooth muscle cells (PASMCs) was evaluated using CCK-8, transwell assay, and inflammatory factor analysis. A four-week hypoxia-induced mouse model of PAH was constructed, and H&E staining, along with Immunofluorescence staining, were employed to assess PAH progression.

Results

Forty-five miRNAs exhibited significant differential expression in TCs following ITGB1 knockdown. Mus-miR-429-3p, significantly upregulated in ITGB1-overexpressing TCs and in ITGB1-modified TC-derived exosomes, was selected for further investigation. Exo-ITGB1 notably inhibited the migration, proliferation, and inflammation of PASMCs by targeting Rac1. Overexpressing Rac1 partly counteracted Exo-ITGB1’s effects. In vivo administration of Exo-ITGB1 effectively reduced pulmonary vascular remodeling and inflammation.

Conclusions

Our findings reveal that ITGB1-modified TC-derived exosomes exert anti-inflammatory effects and reverse vascular remodeling through the miR-429-3p/Rac1 axis. This provides potential therapeutic strategies for PAH treatment.

Graphical Abstract

1. Identification of Differentially Expressed microRNAs (DEmiRs) in ITGB1 overexpressed TCs.

2. Effects of Exo-ITGB1 or miR-429-3p on Hypoxia-Induced PASMCs in vitro.

3. Exo-ITGB1 inhibits the hyper-proliferation and migration of PASMCs through regulating miR-429-3p/Rac1 axis in vitro.

4. The therapeutic potential of Exo-ITGB1 in hypoxia-induced PAH model in vivo.
Gender in Cross-Cultural Encounters: Orientalism and Self-Orientalisation in Chinese-Language Young Adult Fiction
Authors: Shen Lisa Chu , Chen Xianghong
Published: May 2024

In this article, we read and analyse selected young adult novels by Chinese-German author Wei Cheng that address the dilemmas faced by young female Chinese immigrants in Western countries. The aim is to explore the ways in which the construction of femininity and gender are inextricably bound up with categories of race and ethnicity in China-West encounters, and to probe the dynamics of such encounters embedded in the larger socio-political power structures. Through a critical analysis of the representation of Chinese femininity vis-à-vis Western masculinity, the essay interrogates the workings of the “Orientalist gaze” in relation to patriarchy and gender politics. The portrayals of the cross-racial encounter in Shaonv de Hong Chenyi (The Girl’s Red T-Shirt), which reiterate received ideas about the Orient, may be read as a product of self-Orientalisation, where the intriguing position of the adolescent Chinese girl prompts a rethinking of postcolonial gendered subjectivities. While self-Orientalisation may work to legitimate the discourse of Orientalism, the reading of Shaonv de Hong Faqia (The Girl’s Red Hairpin) reveals the ways in which self-Orientalisation could function as strategic essentialism that allows the female “Oriental” Other to gaze back and stand Orientalism on its head. The marriage between gendered and postcolonial approaches to children’s and young adult texts promises to act as a crucial intervention in the global struggle against hegemony.
The interplay between teachers’ trust in artificial intelligence and digital competence
Authors: Lucas Margarida , Zhang Yidi , Bem-haja Pedro , Vicente Paulo Nuno
Published: May 2024

This study examines the relation between K-12 teachers’ trust in artificial intelligence (TAI), their knowledge of AI (KAI), and their digital competence (DC). It further examines the relation between TAI and age, sex, teaching experience and International Standard Classification of Education (ISCED) levels. The study employed a comprehensive and validated instrument and used a sample of 211 primary and secondary school teachers. The results show that there is a significant positive relation between all three variables and that KAI is a robust and substantial predictor of TAI. In the absence of KAI, the significant relation between DC and TAI ceases to exist. In addition, teachers with different levels of DC do not show significant differences in their attitudes towards AI. Results further show that TAI is independent of age, sex, teaching experience and ISCED level in this sample of teachers. The study contributes valuable insights into the complex interplay between teachers’ TAI, their KAI, and their DC, providing practical implications for policy, teacher preparation and professional development in the rapidly evolving landscape of AI integration in education.
Restoration of fumigated soil biota with plant growth-promoting rhizobacteria to counteract Meloidogyne incognita (Tylenchida: Heteroderidae) boosts eggplant growth and defenses
Authors: Ali Abdelhadi A. I. , El-Ashry Ramadan M. , Khairy Ahmed M. , Elakkad Hend A. , Aioub Ahmed A. A. , Ramadan Mahmoud M.
Published: May 2024

This study investigated the effectiveness of two plant growth-promoting rhizobacteria (PGPR) formulations, Bio Arc® 6% WP (BA, Bacillus megaterium) and BECTO Grow Roots® (BGR; Serratia marcescens, Pseudomonas putida, and P. fluorescens). Additionally, it focused on naturally infested soil that had undergone metam potassium fumigation to reduce root-knot nematode (RKN), specifically Meloidogyne incognita. It aimed to monitor eggplant response to soil biodiversity changes for 60 days of the experiment under greenhouse conditions. Our data showed that soil fumigation eliminated nematodes and reduced other microorganisms significantly. In sterilized soil, plant fresh root weight significantly decreased after the elimination of soil biota. Moreover, BGR achieved the highest plant growth vigor [fresh root (18.48%), fresh shoot (23.52%), and fresh plant weight (21.03%)], followed by BA [fresh root (11.98%), fresh shoot (20.77%), and fresh plant weight (16.38%), respectively]. Plant growth traits increased significantly following the second reinoculation of PGPR after a month. Also, the same data trend was observed in treatments inoculated with RKN + PGPR. Furthermore, BGR treatments exhibited consistent efficacy in controlling nematode infection, maintaining superior vegetative growth compared to BA treatments over time. However, BA treatments displayed a decline in plant growth when faced with repeated nematode infection. The range of gall, egg mass, and second-stage juvenile (J2s) numbers diminished after inoculation-infected plants with RKN and PGPR compared with infected plants alone. Biochemical changes in eggplant roots cultivated in sterilized soil exposed significant enhancement in total sugars, protein, and nonenzymatic antioxidants (flavonoids and phenolic content), with antioxidant activity enhanced upon inoculation of PGPR and RKN. Oppositely, the levels of enzymatic antioxidants (catalase, peroxidase, and superoxide dismutase) decreased significantly with single and double PGPR application. However, the decline was less pronounced in the BGR treatments than in BA. In contradiction of oxidative enzymes, reinoculated PGPR + RKN multiplied significantly in reinfested RKN treatments. Our findings support the use of PGPR combinations, in particular, for preventive inoculation against M. incognita. They also enhance plant growth-promoting factors and stimulate plant defenses against inevitable soil reinfestation following soil fumigation.
Turnaround Radius for charged particles in the Reissner–Nordström deSitter spacetime
Authors: German Ethan J. , Sultana Joseph
Published: May 2024

We investigate the turnaround radius of the Reissner–Nordström deSitter Spacetime and how the turnaround radius changes if a test particle carries charge. We also consider the Martínez–Troncoso–Zanelli (MTZ) solution of conformally coupled gravity and investigate how the turnaround radius changes for a scalar test charge. In both scalar and electric interaction cases we find that the Turnaround Radius depends on the particle’s energy.