bluuebunny/crossref_metadata_2025 huggingface dataset:
Opponent vesicular transporters regulate the strength of glutamatergic neurotransmission in a C. elegans sensory circuit
Authors: Choi Jung-Hwan , Horowitz Lauren Bayer , Ringstad Niels
Published: November 2021

At chemical synapses, neurotransmitters are packaged into synaptic vesicles that release their contents in response to depolarization. Despite its central role in synaptic function, regulation of the machinery that loads vesicles with neurotransmitters remains poorly understood. We find that synaptic glutamate signaling in a C. elegans chemosensory circuit is regulated by antagonistic interactions between the canonical vesicular glutamate transporter EAT-4/VGLUT and another vesicular transporter, VST-1. Loss of VST-1 strongly potentiates glutamate release from chemosensory BAG neurons and disrupts chemotaxis behavior. Analysis of the circuitry downstream of BAG neurons shows that excess glutamate release disrupts behavior by inappropriately recruiting RIA interneurons to the BAG-associated chemotaxis circuit. Our data indicate that in vivo the strength of glutamatergic synapses is controlled by regulation of neurotransmitter packaging into synaptic vesicles via functional coupling of VGLUT and VST-1.
Convergence of oncogenic cooperation at single-cell and single-gene levels drives leukemic transformation
Authors: Liu Yuxuan , Gu Zhimin , Cao Hui , Kaphle Pranita , Lyu Junhua , Zhang Yuannyu , Hu Wenhuo , Chung Stephen S. , Dickerson Kathryn E. , Xu Jian
Published: November 2021

Cancers develop from the accumulation of somatic mutations, yet it remains unclear how oncogenic lesions cooperate to drive cancer progression. Using a mouse model harboring NRasG12D and EZH2 mutations that recapitulates leukemic progression, we employ single-cell transcriptomic profiling to map cellular composition and gene expression alterations in healthy or diseased bone marrows during leukemogenesis. At cellular level, NRasG12D induces myeloid lineage-biased differentiation and EZH2-deficiency impairs myeloid cell maturation, whereas they cooperate to promote myeloid neoplasms with dysregulated transcriptional programs. At gene level, NRasG12D and EZH2-deficiency independently and synergistically deregulate gene expression. We integrate results from histopathology, leukemia repopulation, and leukemia-initiating cell assays to validate transcriptome-based cellular profiles. We use this resource to relate developmental hierarchies to leukemia phenotypes, evaluate oncogenic cooperation at single-cell and single-gene levels, and identify GEM as a regulator of leukemia-initiating cells. Our studies establish an integrative approach to deconvolute cancer evolution at single-cell resolution in vivo.
Engineered osteoclasts as living treatment materials for heterotopic ossification therapy
Authors: Jin Wenjing , Lin Xianfeng , Pan Haihua , Zhao Chenchen , Qiu Pengcheng , Zhao Ruibo , Hu Zihe , Zhou Yanyan , Wu Haiyan , Chen Xiao , Ouyang Hongwei , Xie Zhijian , Tang Ruikang
Published: November 2021

Osteoclasts (OCs), the only cells capable of remodeling bone, can demineralize calcium minerals biologically. Naive OCs have limitations for the removal of ectopic calcification, such as in heterotopic ossification (HO), due to their restricted activity, migration and poor adhesion to sites of ectopic calcification. HO is the formation of pathological mature bone within extraskeletal soft tissues, and there are currently no reliable methods for removing these unexpected calcified plaques. In the present study, we develop a chemical approach to modify OCs with tetracycline (TC) to produce engineered OCs (TC-OCs) with an enhanced capacity for targeting and adhering to ectopic calcified tissue due to a broad affinity for calcium minerals. Unlike naive OCs, TC-OCs are able to effectively remove HO both in vitro and in vivo. This achievement indicates that HO can be reversed using modified OCs and holds promise for engineering cells as “living treatment agents” for cell therapy.
Ultrafast high-harmonic nanoscopy of magnetization dynamics
Authors: Zayko Sergey , Kfir Ofer , Heigl Michael , Lohmann Michael , Sivis Murat , Albrecht Manfred , Ropers Claus
Published: November 2021

Light-induced magnetization changes, such as all-optical switching, skyrmion nucleation, and intersite spin transfer, unfold on temporal and spatial scales down to femtoseconds and nanometers, respectively. Pump-probe spectroscopy and diffraction studies indicate that spatio-temporal dynamics may drastically affect the non-equilibrium magnetic evolution. Yet, direct real-space magnetic imaging on the relevant timescales has remained challenging. Here, we demonstrate ultrafast high-harmonic nanoscopy employing circularly polarized high-harmonic radiation for real-space imaging of femtosecond magnetization dynamics. We map quenched magnetic domains and localized spin structures in Co/Pd multilayers with a sub-wavelength spatial resolution down to 16 nm, and strobosocopically trace the local magnetization dynamics with 40 fs temporal resolution. Our compact experimental setup demonstrates the highest spatio-temporal resolution of magneto-optical imaging to date. Facilitating ultrafast imaging with high sensitivity to chiral and linear dichroism, we envisage a wide range of applications spanning magnetism, phase transitions, and carrier dynamics.
Longitudinal single-cell analysis of a myeloma mouse model identifies subclonal molecular programs associated with progression
Authors: Croucher Danielle C. , Richards Laura M. , Tsofack Serges P. , Waller Daniel , Li Zhihua , Wei Ellen Nong , Huang Xian Fang , Chesi Marta , Bergsagel P. Leif , Sebag Michael , Pugh Trevor J. , Trudel Suzanne
Published: November 2021

Molecular programs that underlie precursor progression in multiple myeloma are incompletely understood. Here, we report a disease spectrum-spanning, single-cell analysis of the Vκ*MYC myeloma mouse model. Using samples obtained from mice with serologically undetectable disease, we identify malignant cells as early as 30 weeks of age and show that these tumours contain subclonal copy number variations that persist throughout progression. We detect intratumoural heterogeneity driven by transcriptional variability during active disease and show that subclonal expression programs are enriched at different times throughout early disease. We then show how one subclonal program related to GCN2 stress response is progressively activated during progression in myeloma patients. Finally, we use chemical and genetic perturbation of GCN2 in vitro to support this pathway as a therapeutic target in myeloma. These findings therefore present a model of precursor progression in Vκ*MYC mice, nominate an adaptive mechanism important for myeloma survival, and highlight the need for single-cell analyses to understand the biological underpinnings of disease progression.
Pseudoknot length modulates the folding, conformational dynamics, and robustness of Xrn1 resistance of flaviviral xrRNAs
Authors: Niu Xiaolin , Sun Ruirui , Chen Zhifeng , Yao Yirong , Zuo Xiaobing , Chen Chunlai , Fang Xianyang
Published: November 2021

To understand how RNA dynamics is regulated and connected to its function, we investigate the folding, conformational dynamics and robustness of Xrn1 resistance of a set of flaviviral xrRNAs using SAXS, smFRET and in vitro enzymatic assays. Flaviviral xrRNAs form discrete ring-like 3D structures, in which the length of a conserved long-range pseudoknot (PK2) ranges from 2 bp to 7 bp. We find that xrRNAs’ folding, conformational dynamics and Xrn1 resistance are strongly correlated and highly Mg2+-dependent, furthermore, the Mg2+-dependence is modulated by PK2 length variations. xrRNAs with long PK2 require less Mg2+to stabilize their folding, exhibit reduced conformational dynamics and strong Xrn1 resistance even at low Mg2+, and tolerate mutations at key tertiary motifs at high Mg2+, which generally are destructive to xrRNAs with short PK2. These results demonstrate an unusual regulatory mechanism of RNA dynamics providing insights into the functions and future biomedical applications of xrRNAs.
Striping of orbital-order with charge-disorder in optimally doped manganites
Authors: Chen Wei-Tin , Wang Chin-Wei , Cheng Ching-Chia , Chuang Yu-Chun , Simonov Arkadiy , Bristowe Nicholas C. , Senn Mark S.
Published: November 2021

The phase diagrams of LaMnO3 perovskites have been intensely studied due to the colossal magnetoresistance (CMR) exhibited by compositions around the $${\frac{3}{8}}^{th}$$

3

8

t
h

doping level. However, phase segregation between ferromagnetic (FM) metallic and antiferromagnetic (AFM) insulating states, which itself is believed to be responsible for the colossal change in resistance under applied magnetic field, has prevented an atomistic-level understanding of the orbital ordered (OO) state at this doping level. Here, through the detailed crystallographic analysis of the phase diagram of a prototype system (AMn$${}_{3}^{A^{\prime} }$$

3

A

′

Mn$${}_{4}^{B}$$

4

B

O12), we show that the superposition of two distinct lattice modes gives rise to a striping of OO Jahn-Teller active Mn3+ and charge disordered (CD) Mn3.5+ layers in a 1:3 ratio. This superposition only gives a cancellation of the Jahn-Teller-like displacements at the critical doping level. This striping of CD Mn3.5+ with Mn3+ provides a natural mechanism though which long range OO can melt, giving way to a conducting state.
Structural library and visualization of endogenously oxidized phosphatidylcholines using mass spectrometry-based techniques
Authors: Matsuoka Yuta , Takahashi Masatomo , Sugiura Yuki , Izumi Yoshihiro , Nishiyama Kazuhiro , Nishida Motohiro , Suematsu Makoto , Bamba Takeshi , Yamada Ken-ichi
Published: November 2021

Although oxidized phosphatidylcholines (oxPCs) play critical roles in numerous pathological events, the type and production sites of endogenous oxPCs remain unknown because of the lack of structural information and dedicated analytical methods. Herein, a library of 465 oxPCs is constructed using high-resolution mass spectrometry-based non-targeted analytical methods and employed to detect 70 oxPCs in mice with acetaminophen-induced acute liver failure. We show that doubly oxygenated polyunsaturated fatty acid (PUFA)-PCs (PC PUFA;O2), containing epoxy and hydroxide groups, are generated in the early phase of liver injury. Hybridization with in-vivo 18O labeling and matrix-assisted laser desorption/ionization-tandem MS imaging reveals that PC PUFA;O2 are accumulated in cytochrome P450 2E1-expressing and glutathione-depleted hepatocytes, which are the major sites of liver injury. The developed library and visualization methodology should facilitate the characterization of specific lipid peroxidation events and enhance our understanding of their physiological and pathological significance in lipid peroxidation-related diseases.
Insecticide resistance by a host-symbiont reciprocal detoxification
Authors: Sato Yuya , Jang Seonghan , Takeshita Kazutaka , Itoh Hideomi , Koike Hideaki , Tago Kanako , Hayatsu Masahito , Hori Tomoyuki , Kikuchi Yoshitomo
Published: November 2021

Insecticide resistance is one of the most serious problems in contemporary agriculture and public health. Although recent studies revealed that insect gut symbionts contribute to resistance, the symbiont-mediated detoxification process remains unclear. Here we report the in vivo detoxification process of an organophosphorus insecticide, fenitrothion, in the bean bug Riptortus pedestris. Using transcriptomics and reverse genetics, we reveal that gut symbiotic bacteria degrade this insecticide through a horizontally acquired insecticide-degrading enzyme into the non-insecticidal but bactericidal compound 3-methyl-4-nitrophenol, which is subsequently excreted by the host insect. This integrated “host-symbiont reciprocal detoxification relay” enables the simultaneous maintenance of symbiosis and efficient insecticide degradation. We also find that the symbiont-mediated detoxification process is analogous to the insect genome-encoded fenitrothion detoxification system present in other insects. Our findings highlight the capacity of symbiosis, combined with horizontal gene transfer in the environment, as a powerful strategy for an insect to instantly eliminate a toxic chemical compound, which could play a critical role in the human-pest arms race.
Forest defoliator outbreaks alter nutrient cycling in northern waters
Authors: Woodman Samuel G. , Khoury Sacha , Fournier Ronald E. , Emilson Erik J. S. , Gunn John M. , Rusak James A. , Tanentzap Andrew J.
Published: November 2021

Insect defoliators alter biogeochemical cycles from land into receiving waters by consuming terrestrial biomass and releasing biolabile frass. Here, we related insect outbreaks to water chemistry across 12 boreal lake catchments over 32-years. We report, on average, 27% lower dissolved organic carbon (DOC) and 112% higher dissolved inorganic nitrogen (DIN) concentrations in lake waters when defoliators covered entire catchments and reduced leaf area. DOC reductions reached 32% when deciduous stands dominated. Within-year changes in DOC from insect outbreaks exceeded 86% of between-year trends across a larger dataset of 266 boreal and north temperate lakes from 1990 to 2016. Similarly, within-year increases in DIN from insect outbreaks exceeded local, between-year changes in DIN by 12-times, on average. As insect defoliator outbreaks occur at least every 5 years across a wider 439,661 km2boreal ecozone of Ontario, we suggest they are an underappreciated driver of biogeochemical cycles in forest catchments of this region.
Aerosol-boundary-layer-monsoon interactions amplify semi-direct effect of biomass smoke on low cloud formation in Southeast Asia
Authors: Ding Ke , Huang Xin , Ding Aijun , Wang Minghuai , Su Hang , Kerminen Veli-Matti , Petäjä Tuukka , Tan Zhemin , Wang Zilin , Zhou Derong , Sun Jianning , Liao Hong , Wang Huijun , Carslaw Ken , Wood Robert , Zuidema Paquita , Rosenfeld Daniel , Kulmala Markku , Fu Congbin , Pöschl Ulrich , Cheng Yafang , Andreae Meinrat O.
Published: November 2021

Low clouds play a key role in the Earth-atmosphere energy balance and influence agricultural production and solar-power generation. Smoke aloft has been found to enhance marine stratocumulus through aerosol-cloud interactions, but its role in regions with strong human activities and complex monsoon circulation remains unclear. Here we show that biomass burning aerosols aloft strongly increase the low cloud coverage over both land and ocean in subtropical southeastern Asia. The degree of this enhancement and its spatial extent are comparable to that in the Southeast Atlantic, even though the total biomass burning emissions in Southeast Asia are only one-fifth of those in Southern Africa. We find that a synergetic effect of aerosol-cloud-boundary layer interaction with the monsoon is the main reason for the strong semi-direct effect and enhanced low cloud formation in southeastern Asia.
A molecular vision of fungal cell wall organization by functional genomics and solid-state NMR
Authors: Chakraborty Arnab , Fernando Liyanage D. , Fang Wenxia , Dickwella Widanage Malitha C. , Wei Pingzhen , Jin Cheng , Fontaine Thierry , Latgé Jean-Paul , Wang Tuo
Published: November 2021

Vast efforts have been devoted to the development of antifungal drugs targeting the cell wall, but the supramolecular architecture of this carbohydrate-rich composite remains insufficiently understood. Here we compare the cell wall structure of a fungal pathogen Aspergillus fumigatus and four mutants depleted of major structural polysaccharides. High-resolution solid-state NMR spectroscopy of intact cells reveals a rigid core formed by chitin, β-1,3-glucan, and α-1,3-glucan, with galactosaminogalactan and galactomannan present in the mobile phase. Gene deletion reshuffles the composition and spatial organization of polysaccharides, with significant changes in their dynamics and water accessibility. The distribution of α-1,3-glucan in chemically isolated and dynamically distinct domains supports its functional diversity. Identification of valines in the alkali-insoluble carbohydrate core suggests a putative function in stabilizing macromolecular complexes. We propose a revised model of cell wall architecture which will improve our understanding of the structural response of fungal pathogens to stresses.
Oral prodrug of remdesivir parent GS-441524 is efficacious against SARS-CoV-2 in ferrets
Authors: Cox Robert M. , Wolf Josef D. , Lieber Carolin M. , Sourimant Julien , Lin Michelle J. , Babusis Darius , DuPont Venice , Chan Julie , Barrett Kim T. , Lye Diane , Kalla Rao , Chun Kwon , Mackman Richard L. , Ye Chengjin , Cihlar Tomas , Martinez-Sobrido Luis , Greninger Alexander L. , Bilello John P. , Plemper Richard K.
Published: November 2021

Remdesivir is an antiviral approved for COVID-19 treatment, but its wider use is limited by intravenous delivery. An orally bioavailable remdesivir analog may boost therapeutic benefit by facilitating early administration to non-hospitalized patients. This study characterizes the anti-SARS-CoV-2 efficacy of GS-621763, an oral prodrug of remdesivir parent nucleoside GS-441524. Both GS-621763 and GS-441524 inhibit SARS-CoV-2, including variants of concern (VOC) in cell culture and human airway epithelium organoids. Oral GS-621763 is efficiently converted to plasma metabolite GS-441524, and in lungs to the triphosphate metabolite identical to that generated by remdesivir, demonstrating a consistent mechanism of activity. Twice-daily oral administration of 10 mg/kg GS-621763 reduces SARS-CoV-2 burden to near-undetectable levels in ferrets. When dosed therapeutically against VOC P.1 gamma γ, oral GS-621763 blocks virus replication and prevents transmission to untreated contact animals. These results demonstrate therapeutic efficacy of a much-needed orally bioavailable analog of remdesivir in a relevant animal model of SARS-CoV-2 infection.
Temporal changes in anthropogenic seismic noise levels associated with economic and leisure activities during the COVID-19 pandemic
Authors: Nimiya Hiro , Ikeda Tatsunori , Tsuji Takeshi
Published: November 2021

Seismic ambient noise with frequencies > 1 Hz includes noise related to human activities. A reduction in seismic noise during the COVID-19 pandemic has been observed worldwide, as restrictions were imposed to control outbreaks of the SARS-CoV-2 virus. In this context, we studied the effect of changes in anthropogenic activities during COVID-19 on the seismic noise levels in the Tokyo metropolitan area, Japan, considering time of day, day of the week, and seasonal changes. The results showed the largest reduction in noise levels during the first state of emergency under most conditions. After the first state of emergency was lifted, the daytime noise reverted to previous levels immediately on weekdays and gradually on Sundays. This was likely because economic activities instantly resumed, while non-essential outings on Sundays were still mostly avoided. Furthermore, the daytime noise level on Sundays was strongly reduced regardless of changes on weekdays after the second state of emergency, which restricted activities mainly at night. Sunday noise levels gradually increased from the middle of the second state of emergency, suggesting a gradual reduction in public concern about COVID-19 following a decrease in the number of infections. Our findings demonstrate that seismic noise can be used to monitor social activities.
Excluding false negative error in certification of quantum channels
Authors: Krawiec Aleksandra , Pawela Łukasz , Puchała Zbigniew
Published: November 2021

Certification of quantum channels is based on quantum hypothesis testing and involves also preparation of an input state and choosing the final measurement. This work primarily focuses on the scenario when the false negative error cannot occur, even if it leads to the growth of the probability of false positive error. We establish a condition when it is possible to exclude false negative error after a finite number of queries to the quantum channel in parallel, and we provide an upper bound on the number of queries. On top of that, we found a class of channels which allow for excluding false negative error after a finite number of queries in parallel, but cannot be distinguished unambiguously. Moreover, it will be proved that parallel certification scheme is always sufficient, however the number of steps may be decreased by the use of adaptive scheme. Finally, we consider examples of certification of various classes of quantum channels and measurements.
Home advantage mediated (HAM) by referee bias and team performance during covid
Authors: Bilalić Merim , Gula Bartosz , Vaci Nemanja
Published: November 2021

The fans’ importance in sports is acknowledged by the term ‘the 12th man’, a figurative extra player for the home team. Sport teams are indeed more successful when they play in front of their fans than when they play away. The supposed mechanism behind this phenomenon, termed Home Advantage (HA), is that fans’ support spurs home players to better performance and biases referees, which in turn determines the outcome. The inference about the importance of fans’ support is, however, indirect as there is normally a 12th man of this kind, even if it is an opponent’s. The current pandemic, which forced sporting activities to take place behind closed doors, provides the necessary control condition. Here we employ a novel conceptual HA model on a sample of over 4000 soccer matches from 12 European leagues, some played in front of spectators and some in empty stadia, to demonstrate that fans are indeed responsible for the HA. However, the absence of fans reduces the HA by a third, as the home team’s performance suffers and the officials’ bias disappears. The current pandemic reveals that the figurative 12th man is no mere fan hyperbole, but is in fact the most important player in the home team.
Defluorination and adsorption of tetrafluoroethylene (TFE) on TiO2(110) and Cr2O3(0001)
Authors: Gueriba Jessiel Siaron , Salehuddin Nur Ellina Annisa , Diño Wilson Agerico , Washika Kiminori , Nakamura Hiroshi , Kawafuchi Tatsumi
Published: November 2021

Here, we show that metal oxide surfaces catalyze the formation of intermediate defluorinated tetrafluoroethylene (TFE) radicals, resulting in enhanced binding on the corresponding metal oxide surfaces. We attribute the preferential adsorption and radical formation of TFE on Cr2O3(0001) relative to TiO2(110) to the low oxygen coordination of Cr surface atoms. This hints at a possible dependence of the TFE binding strength to the surface stoichiometry of metal-oxide surfaces.
High quality VO2 thin films synthesized from V2O5 powder for sensitive near-infrared detection
Authors: Guo Xitao , Tan Yonghao , Hu Yupei , Zafar Zainab , Liu Jun , Zou Jijun
Published: November 2021

Vapor transport method has been successfully used to synthesize high quality VO2 thin films on SiO2/Si substrate using V2O5 as a precursor in an inert-gas environment. The morphological and structural evolutions of the intermediate phases during the nucleation and growth processes were investigated by SEM and Raman spectroscopy, respectively. The results showed that the conversion of V2O5 powder to VO2 thin films was dominated by a melting-evaporation-nucleation-growth mechanism. Further characterization results demonstrated that the high quality crystals of monoclinic VO2 thin films exhibit a sharp resistance change up to 4 orders of magnitude. In addition, the VO2 thin films exhibited good near-infrared response, high stability, and reproducibility under ambient conditions, which should be promising for sensitive near-infrared detection. Our work not only provided a simple and direct approach to synthesize high quality VO2 thin films with distinct phase transition properties but also demonstrated the possible infrared sensing application in the future.
Boosting the resolution of multidimensional NMR spectra by complete removal of proton spin multiplicities
Authors: Sakhaii Peyman , Bohorc Bojan , Schliedermann Uwe , Bermel Wolfgang
Published: November 2021

Over decades multidimensional NMR spectroscopy has become an indispensable tool for structure elucidation of natural products, peptides and medium sized to large proteins. Heteronuclear single quantum coherence (HSQC) spectroscopy is one of the work horses in that field often used to map structural connectivity between protons and carbons or other hetero nuclei. In overcrowded HSQC spectra, proton multiplet structures of cross peaks set a limit to the power of resolution and make a straightforward assignment difficult. In this work, we provide a solution to improve these penalties by completely removing the proton spin multiplet structure of HSQC cross peaks. Previously reported sideband artefacts are diminished leading to HSQC spectra with singlet responses for all types of proton multiplicities. For sideband suppression, the idea of restricted random delay (RRD) in chunk interrupted data acquisition is introduced and exemplified. The problem of irreducible residual doublet splitting of diastereotopic CH2 groups is simply solved by using a phase sensitive JRES approach in conjunction with echo processing and real time broadband homodecoupling (BBHD) HSQC, applied as a 3D experiment. Advantages and limitations of the method is presented and discussed.
Association between thyroid hormones and insulin resistance indices based on the Korean National Health and Nutrition Examination Survey
Authors: Choi Yun Mi , Kim Min Kyung , Kwak Mi Kyung , Kim Dooman , Hong Eun-Gyoung
Published: November 2021

Thyroid dysfunction has been implicated as a potential pathophysiological factor in glucose homeostasis and insulin resistance (IR). This study aimed to identify the correlation between thyroid dysfunction and IR. We used data from the sixth Korean National Health and Nutrition Examination Survey to evaluate a total of 5727 participants. The triglyceride glucose (TyG) index and homeostasis model assessment of insulin resistance (HOMA-IR) were calculated to represent IR. Correlation analysis was performed between thyroid dysfunction and IR. The log-transformed TSH (LnTSH) and free T4 were significantly correlated with the TyG index (TSH, beta coefficient 0.025, 95% confidence interval [CI] 0.014–0.036, p < 0.001; free T4, − 0.110 (− 0.166 to − 0.054), p < 0.001) but not HOMA-IR. Overt hypothyroidism is correlated with increased TyG index in pre-menopausal females (0.215 (0.122–0.309) p < 0.001). On the other hand, overt hyperthyroidism is correlated with increased HOMA-IR in males (0.304 (0.193–0.416), p < 0.001) and post-menopausal females (1.812 (1.717–1.907), p < 0.001). In euthyroid subjects, LnTSH and TyG index were significantly correlated in females. In conclusion, both hyperthyroidism and hypothyroidism might be associated with IR but by different mechanisms. It might be helpful to assess IR with appropriate indexes in patients with thyroid dysfunction.
Shortwave infrared hyperspectral imaging as a novel method to elucidate multi-phase dolomitization, recrystallization, and cementation in carbonate sedimentary rocks
Authors: McCormick Cole A. , Corlett Hilary , Stacey Jack , Hollis Cathy , Feng Jilu , Rivard Benoit , Omma Jenny E.
Published: November 2021

Carbonate rocks undergo low-temperature, post-depositional changes, including mineral precipitation, dissolution, or recrystallisation (diagenesis). Unravelling the sequence of these events is time-consuming, expensive, and relies on destructive analytical techniques, yet such characterization is essential to understand their post-depositional history for mineral and energy exploitation and carbon storage. Conversely, hyperspectral imaging offers a rapid, non-destructive method to determine mineralogy, while also providing compositional and textural information. It is commonly employed to differentiate lithology, but it has never been used to discern complex diagenetic phases in a largely monomineralic succession. Using spatial-spectral endmember extraction, we explore the efficacy and limitations of hyperspectral imaging to elucidate multi-phase dolomitization and cementation in the Cathedral Formation (Western Canadian Sedimentary Basin). Spectral endmembers include limestone, two replacement dolomite phases, and three saddle dolomite phases. Endmember distributions were mapped using Spectral Angle Mapper, then sampled and analyzed to investigate the controls on their spectral signatures. The absorption-band position of each phase reveals changes in %Ca (molar Ca/(Ca + Mg)) and trace element substitution, whereas the spectral contrast correlates with texture. The ensuing mineral distribution maps provide meter-scale spatial information on the diagenetic history of the succession that can be used independently and to design a rigorous sampling protocol.
Spatio-temporal prediction of the COVID-19 pandemic in US counties: modeling with a deep LSTM neural network
Authors: Nikparvar Behnam , Rahman Md. Mokhlesur , Hatami Faizeh , Thill Jean-Claude
Published: November 2021

Prediction of complex epidemiological systems such as COVID-19 is challenging on many grounds. Commonly used compartmental models struggle to handle an epidemiological process that evolves rapidly and is spatially heterogeneous. On the other hand, machine learning methods are limited at the beginning of the pandemics due to small data size for training. We propose a deep learning approach to predict future COVID-19 infection cases and deaths 1 to 4 weeks ahead at the fine granularity of US counties. The multi-variate Long Short-term Memory (LSTM) recurrent neural network is trained on multiple time series samples at the same time, including a mobility series. Results show that adding mobility as a variable and using multiple samples to train the network improve predictive performance both in terms of bias and of variance of the forecasts. We also show that the predicted results have similar accuracy and spatial patterns with a standard ensemble model used as benchmark. The model is attractive in many respects, including the fine geographic granularity of predictions and great predictive performance several weeks ahead. Furthermore, data requirement and computational intensity are reduced by substituting a single model to multiple models folded in an ensemble model.
PER2 mediates CREB-dependent light induction of the clock gene Per1
Authors: Brenna Andrea , Ripperger Jürgen A. , Saro Gabriella , Glauser Dominique A. , Yang Zhihong , Albrecht Urs
Published: November 2021

Light affects many physiological processes in mammals such as entrainment of the circadian clock, regulation of mood, and relaxation of blood vessels. At the molecular level, a stimulus such as light initiates a cascade of kinases that phosphorylate CREB at various sites, including serine 133 (S133). This modification leads CREB to recruit the co-factor CRCT1 and the histone acetyltransferase CBP to stimulate the transcription of genes containing a CRE element in their promoters, such as Period 1 (Per1). However, the details of this pathway are poorly understood. Here we provide evidence that PER2 acts as a co-factor of CREB to facilitate the formation of a transactivation complex on the CRE element of the Per1 gene regulatory region in response to light or forskolin. Using in vitro and in vivo approaches, we show that PER2 modulates the interaction between CREB and its co-regulator CRTC1 to support complex formation only after a light or forskolin stimulus. Furthermore, the absence of PER2 abolished the interaction between the histone acetyltransferase CBP and CREB. This process was accompanied by a reduction of histone H3 acetylation and decreased recruitment of RNA Pol II to the Per1 gene. Collectively, our data show that PER2 supports the stimulus-dependent induction of the Per1 gene via modulation of the CREB/CRTC1/CBP complex.
Molecular species delimitation refines the taxonomy of native and nonnative physinine snails in North America
Authors: Young Michael K. , Smith Rebecca , Pilgrim Kristine L. , Schwartz Michael K.
Published: November 2021

Being able to associate an organism with a scientific name is fundamental to our understanding of its conservation status, ecology, and evolutionary history. Gastropods in the subfamily Physinae have been especially troublesome to identify because morphological variation can be unrelated to interspecific differences and there have been widespread introductions of an unknown number of species, which has led to a speculative taxonomy. To resolve uncertainty about species diversity in North America, we targeted an array of single-locus species delimitation methods at publically available specimens and new specimens collected from the Snake River basin, USA to generate species hypotheses, corroborated using nuclear analyses of the newly collected specimens. A total-evidence approach delineated 18 candidate species, revealing cryptic diversity within recognized taxa and a lack of support for other named taxa. Hypotheses regarding certain local endemics were confirmed, as were widespread introductions, including of an undescribed taxon likely belonging to a separate genus in southeastern Idaho for which the closest relatives are in southeast Asia. Overall, single-locus species delimitation was an effective first step toward understanding the diversity and distribution of species in Physinae and to guiding future investigation sampling and analyses of species hypotheses.
Modulation of spin-torque ferromagnetic resonance with a nanometer-thick platinum by ionic gating
Authors: Ohshima Ryo , Kohsaka Yuto , Ando Yuichiro , Shinjo Teruya , Shiraishi Masashi
Published: November 2021

The spin Hall effect (SHE) and inverse spin Hall effect (ISHE) have played central roles in modern condensed matter physics especially in spintronics and spin-orbitronics, and much effort has been paid to fundamental and application-oriented research towards the discovery of novel spin–orbit physics and the creation of novel spintronic devices. However, studies on gate-tunability of such spintronics devices have been limited, because most of them are made of metallic materials, where the high bulk carrier densities hinder the tuning of physical properties by gating. Here, we show an experimental demonstration of the gate-tunable spin–orbit torque in Pt/Ni80Fe20 (Py) devices by controlling the SHE using nanometer-thick Pt with low carrier densities and ionic gating. The Gilbert damping parameter of Py and the spin-memory loss at the Pt/Py interface were modulated by ionic gating to Pt, which are compelling results for the successful tuning of spin–orbit interaction in Pt.
Separation of Gagua Rise from Great Benham Rise in the West Philippine Basin during the Middle Eocene
Authors: Yeh Yi-Ching , Lin Jing-Yi , Hsu Shu-Kun , Tsai Ching-Hui , Chang Ching-Min
Published: November 2021

The West Philippine Basin (WPB) has started opening at ~ 58 Ma and ceased spreading at ~ 33 Ma, developing a fast spreading (~ 44 mm/yr half-spreading rate) magmatic episode between 58 and 41 Ma and the second amagmatic episode between 41 and 33 Ma. The occurrence of the first stage of spreading is closely related to the Oki-Daito mantle plume and related Benham Rise (BR) and Urdaneta Plateau (UP) activity. To the east of the Luzon–Okinawa Fracture Zone (LOFZ), BR was the most active volcanism from 48 to 41 Ma. The geomagnetic ages on both sides of the LOFZ have been determined; however, their causal relationship and evolution in the WPB remain unclear. In this study, we performed integrated analyses of multichannel seismic data and swath bathymetry data for the area to the west of the LOFZ. To the west of the LOFZ, the Gagua Rise (GR), is identified by a high residual free-air gravity anomaly, volcanic seamount chains and an overlapping spreading center. The GR is located at magnetic isochrons C20/C22 (50 to 44 Ma) and shows a thick oceanic crust of at least 12.7 km. We first propose an oceanic plateau named Great Benham Rise (GBR) which includes GR, UP and BR. We infer that the GR was a portion of the GBR since ~ 49 Ma and was separated from the GBR at ~ 41 Ma by the right-lateral LOFZ motion. Later, the relict GBR magmatism only continued in the area to the east of the LOFZ. Overall, the GBR dominates the spreading history of the WPB.
Future of total hip arthroplasty with the Metha short stem in modern surgeries
Authors: Drobniewski Marek , Synder Marek , Synder Marek Aleksander , Krasińska Magdalena , Olewnik Lukasz , Borowski Andrzej
Published: November 2021

The aim of the study was to analyse the results of uncemented total hip replacement, using the Metha (metaphyseal) stem. A total of 158 patients (70 females and 88 males) were qualified to the study and submitted to total hip arthroplasty (183 procedures altogether), using the Metha stem. The mean age of the patients on the day of surgery was 51.7 years (the range from 17 to 69 years). The mean follow up period was 9.2 years (the range from 5 to 13.5 years). Preoperative assessments gave poor scores, according to the Merle d’Aubigne and Postel classification, modified by Charnley. The average improvement after surgery, according to the used scale, was 6.9 points. A very good outcome was recorded in 154 cases (84.2%), a good outcome was achieved in 20 cases (10.9%) and a poor outcome was confirmed in 9 cases, while no satisfactory case was observed. Poor outcomes were associated with implant loosening. Extraskeletal ossification was noted in 10 cases (5.5%). According to the Kaplan–Meier estimator, the 10-year survival was 93.2% and 97.3% for the whole implant and the stem alone, respectively. 1. Our follow-up period of more than 9 years on the average, indicates that Metha stems produce excellent clinical and functional results in operated young patients with advanced degenerative changes of the hip joint. 2. Assuming a proper qualification for the procedure, the absence of complications and a correct surgical technique, which is slightly more difficult, when compared to standard stem implantation, the risk of aseptic loosening is fairly negligible.
Splenectomy is associated with sexual dysfunctions and decreased libido
Authors: Sabino Kelly Renata , Petroianu Andy
Published: November 2021

The removal of the spleen due to disease or trauma may be followed by disorders due to the asplenism, including immunodeficiency, hematological and metabolic diseases, mainly dyslipidemia, which can lead to sepsis, pulmonary embolism and early death. Although patients frequently report sexual disinterest after splenectomy, this feature has been experimentally studied only in a translational investigation performed by the same group of this work. To study libido and other sexual functions after the complete removal of the spleen in humans. This study was performed on 60 healthy adults, 30 men and 30 women, after more than 1 year of total splenectomy to treat isolated splenic trauma. The International Index of Erectile Function was applied to men and the Female Sexual Function Index to women. The analysis compared the responses obtained in the periods before and after the splenectomy. Laboratory tests with hematological and biochemical analyses, including sex hormones, were performed in all patients. The pre- and postoperative results were compared in each group using the paired t-test, with each patient being his or her own control and with significance to p < 0.05. A decrease in libido and an increase in sexual dysfunction was observed after splenectomy in all men and women, p < 0.001. All postoperative laboratory tests showed normal values in both genders. Asplenia is associated with a marked decrease in libido and intense sexual dysfunction in both men and women, with normal hematological and biochemical laboratory tests, including hormonal exams.
Effect of sutureless securement on hemodialysis catheter-related bloodstream infection
Authors: Fujimoto Keiji , Iida Emi , Kumano Syo , Fujii Ai , Adachi Hiroki , Furuichi Kengo , Yokoyama Hitoshi
Published: November 2021

The use of sutureless securement devices during catheterization might reduce the risk of catheter-related bloodstream infection (CRBSI) by suppressing catheter-exit infection and catheter dislodgement. However, the effectiveness of these devices in reducing CRBSI risk when securing hemodialysis catheters has not been explored. This single-center retrospective observational study examined 211 non-tunneled hemodialysis catheters (NTHCs) from 110 hemodialysis inpatients, of which 121 were secured using conventional skin sutures (Suture group) and 90 with GRIP-LOK (GRIP-LOK group). The stabilized inverse probability of treatment (SIPT)-weighting method was used to generate a new population (SIPT-weighted model) without group differences for each of the 12 predictors of CRBSI development (i.e., age, sex, dialysis history, concomitant acute kidney injury or diabetes, concurrent use of immunosuppressant drugs or aspirin, NTHC insertion site, methicillin-resistant Staphylococcus aureus, carriage, bacteremia event within 3 months before catheterization, hemoglobin level, and serum albumin titer). The effect of GRIP-LOK compared with sutures on CRBSI in the SIPT-weighted model was evaluated using univariate SIPT-weighted Cox proportional regression analysis, which showed a significant CRBSI suppression effect of GRIP-LOK compared with sutures (hazard ratio: 0.17 [95% CI 0.04–0.78], p = 0.023).　GRIP-LOK affords a lower risk of CRBSI due to indwelling NTHCs than conventional securement using sutures.
Effects of whole-body vibration training in a cachectic C26 mouse model
Authors: van der Ende Miranda , Plas Rogier L. C. , van Dijk Miriam , Dwarkasing Jvalini T. , van Gemerden Frans , Sarokhani Attusa , Swarts Hans J. M. , van Schothorst Evert M. , Grefte Sander , Witkamp Renger F. , van Norren Klaske
Published: November 2021

Targeted exercise combined with nutritional and pharmacological strategies is commonly considered to be the most optimal strategy to reduce the development and progression of cachexia. For COPD patients, this multi-targeted treatment has shown beneficial effects. However, in many, physical activity is seriously hampered by frailty and fatigue. In the present study, effects of whole-body-vibration-training (WBV) were investigated, as potential alternative to active exercise, on body mass, muscle mass and function in tumour bearing mice. Twenty-four male CD2F1-mice (6–8 weeks, 21.5 ± 0.2 g) were stratified into four groups: control, control + WBV, C26 tumour-bearing, and C26 tumour-bearing + WBV. From day 1, whole-body-vibration was daily performed for 19 days (15 min, 45 Hz, 1.0 g acceleration). General outcome measures included body mass and composition, daily activity, blood analysis, assessments of muscle histology, function, and whole genome gene expression in m. soleus (SOL), m. extensor digitorum longus (EDL), and heart. Body mass, lean and fat mass and EDL mass were all lower in tumour bearing mice compared to controls. Except from improved contractility in SOL, no effects of vibration training were found on cachexia related general outcomes in control or tumour groups, as PCA analysis did not result in a distinction between corresponding groups. However, analysis of transcriptome data clearly revealed a distinction between tumour and trained tumour groups. WBV reduced the tumour-related effects on muscle gene expression in EDL, SOL and heart. Gene Set Enrichment Analysis showed that these effects were associated with attenuation of the upregulation of the proteasome pathway in SOL. These data suggest that WBV had minor effects on cachexia related general outcomes in the present experimental set-up, while muscle transcriptome showed changes associated with positive effects. This calls for follow-up studies applying longer treatment periods of WBV as component of a multiple-target intervention.
Electro-spinning of highly-aligned polyacrylonitrile nano-fibres with continuous spooling
Authors: Shao Siheng , Ma Tao , Fernando Gerard F.
Published: November 2021

This paper reports on a new configuration for producing highly-aligned electro-spun fibres that can be produced on a static substrate or one where it is hauled off and spooled continuously to enable the production of continuous lengths. The fixture consists of a Vee-shaped polytetrafluorethylene shield at 60° with a 1 cm wide integral rectangular base that is mounted on a copper disk with a 10 cm diameter. Specified concentrations of polyacrylonitrile in dimethyl sulfoxide were electro-spun on to a strip of cellulose paper. In the static setup, approximately 91% of the fibres were deposited to within 3°. When the spooling rig was used, a tape of the cellulose paper was hauled off at 0.07 mm/min, 78% of the fibres were aligned to within 3°. Simulations of the conventional and Vee-shield electro-spinning setups were undertaken and they provided corroboration for the experimental observations with regard to the mechanism responsible for fibre alignment. The feasibility of using this technique to produce 0°/− 45°/+ 45° stacked layers of aligned fibre preform is demonstrated.
Non-enzymatic colorimetric detection of hydrogen peroxide using a μPAD coupled with a machine learning-based smartphone app
Authors: Doğan Vakkas , Yüzer Elif , Kılıç Volkan , Şen Mustafa
Published: November 2021

Here, a smartphone app named Hi-perox Sens supported by machine learning classifiers was applied to a μPAD based on an iodide-mediated TMB-H2O2 reaction system for non-enzymatic colorimetric determination of H2O2.
Cyclic peptide drugs approved in the last two decades (2001–2021)
Authors: Zhang Huiya , Chen Shiyu
Published: November 2021

In this mini-review, we summarized the chemical structure, mechanism of action, and metabolism of cyclic peptide drugs approved in the last two decades. We also examined factors important for the development and utilization in clinical situations.
Boosted Li+ transference number enabled via interfacial engineering for dendrite-free lithium metal anodes
Authors: Zeng Ting , Yan Yu , He Miao , Zheng Ruixin , Du Dayue , Ren Longfei , Zhou Bo , Shu Chaozhu
Published: November 2021

The unsaturated coordinative metal sites in ZIF-67 tend to adsorb anions in the electrolyte, thus increasing the Li+ transference number.
Controlling 1T/2H heterophase junctions in the MoS2 microsphere for the highly efficient photocatalytic hydrogen evolution
Authors: Li Qiuzhong , Huang Lin , Dai Wenxin , Zhang Zizhong
Published: November 2021

The 1T/2H heterophase junction MoS2 microspheres were fabricated for a highly efficient photocatalytic hydrogen evolution.
Mass production of thermally stable Pt single-atom catalysts for the catalytic oxidation of sulfur dioxide
Authors: Jianchen Wang , Yong Kang , Sun Fangkuan
Published: November 2021

Owing to the 100% atom utilization and the potential in bridging the gap between homogeneous catalysis and heterogeneous catalysis, single-atom catalysts (SACs) have doubtlessly obtained broad attention from both academia and industry.
pH-Controlled assembly of [ZnW12O40]6−-based hybrids from a 0D dimer to a 2D network: synthesis, crystal structure, and photocatalytic performance in transformation of toluene into benzaldehyde
Authors: Guo Ke-Ke , Yang Yan-Li , Dong Si-Meng , Li Feng-Yan , Jiang Xin-Ye , Xu Lin
Published: November 2021

Three new ZnW12-based hybrids were synthesized by controlling the pH values of the reaction systems. Compound 3 can efficiently catalyze the oxidation of toluene to benzaldehyde.
From ferrocene to 1,2,3,4,5-pentafluoroferrocene: halogen effect on the properties of metallocene
Authors: Erb William , Richy Nicolas , Hurvois Jean-Pierre , Low Paul J. , Mongin Florence
Published: November 2021

Impact of successive fluorinations onto redox, spectroscopic and NMR properties of ferrocene.
High-performance n-type Ta4SiTe4/polyvinylidene fluoride (PVDF)/graphdiyne organic–inorganic flexible thermoelectric composites
Authors: Qu Sanyin , Ming Chen , Qiu Pengfei , Xu Kunqi , Xu Qing , Yao Qin , Lu Ping , Zeng Huarong , Shi Xun , Chen Lidong
Published: November 2021

Beyond carbon nanotubes and graphene, we conduct a proof-of-principle study by using semiconducting graphdiyne (GDY) to fabricate high-performance n-type organic–inorganic flexible thermoelectric composites.