bluuebunny/crossref_metadata_2025 huggingface dataset:
The Characterization of Biodegradable Films and Food Packaging
Authors: Marcet Ismael
Published: October 2023

Every year, approximately 300 million tons of petroleum-based plastics is manufactured worldwide, and these plastics cause significant environmental issues due to their non-biodegradable nature and emission of toxic gases upon incineration [...]
Efficient Sintering of Mo Matrix Composites—A Study of Temperature Dependences and the Use of the Sinter Additive Ni
Authors: Solodkyi Ievgen , Petrusha Vadym , Grigoroscuta Mihai Alexandru , Schmelzer Janett , Hasemann Georg , Betke Ulf , Badica Petre , Krüger Manja
Published: October 2023

Mo matrix composites (MMC) with Mo-9Si-8B inclusions were fabricated by pressure-less sintering (PLS) and spark plasma sintering (SPS) techniques at temperatures between 1200–1500 °C using 1 wt.% Ni sinter additive. The positive impact of the addition Ni addition on the sinterability and formation of a continuous Mo matrix of MMC with randomly distributed Mo3Si and Mo5SiB2 inclusions was determined. The Ni addition increased the shrinkage of MMC during PLS by almost a third. The continuous Mo matrix of MMC and a relative density of more than 98% was obtained after SPS at 1400–1500 °C. The composite with the maximum relative density of 98% showed a Vickers hardness of 482 ± 9 (HV20). The potential of using Ni-activated PLS and SPS to produce high-density MMC is shown.
An Improved Correlation for the Estimation of the Yield Strength from Small Punch Testing
Authors: Altstadt Eberhard
Published: October 2023

This study aims at improving the empirical correlation for estimating the yield strength from small punch tests. The currently used procedure in the European standard EN 10371 to determine the elastic–plastic transition force—based on bi-linear fitting—involves a dependency not only on the onset of plastic flow but also on the work hardening of the material. Consequently, the yield strength correlation factor is not universal but depends on the material properties and on the geometry of the small punch set-up, leading to a significant uncertainty in the yield strength estimation. In this study, an alternative definition of the elastic–plastic transition force is proposed, which depends significantly less on the work hardening of the material and on the small punch geometry. The approach is based on extensive elastic–plastic finite element simulations with generic material properties, including a systematic variation of the yield strength, ultimate tensile strength, and uniform elongation. The new definition of the transition force is based on the deviation of the force-deflection curve from the analytical elastic slope derived by Reissner’s plate theory. A significant reduction of the uncertainty of the yield strength estimation is demonstrated.
Influence of Inner Roller Geometric Parameters on Counter-Roller Spinning with 6061 Aluminum Alloy Tube
Authors: Zhao Xiaokai , Mu Zheyuan , Zhao Haopeng , Wang Pengyi , Song Wenjie , Yang Guang
Published: October 2023

The inner roller exerts a supportive and thinning effect on the inner side of the tube during counter-roller spinning. In this paper, the Finite Element Analysis (FEA) model of counter-roller spinning for a 6061 aluminum alloy tube was established based on the ABAQUS/Explicit module. The deformation characteristics and the influence of inner roller geometrical parameters on the tube spinning were analyzed. The results showed that the stress–strain on the outer of the tube was greater than that of the inner, and flaring was more prone to occur in the initial stage of counter-roller spinning compared to traditional mandrel spinning. The order of the effects of geometrical parameters of the inner roller on the roundness error and wall thickness deviation was as follows: nose radius > diameter > front angle. The order of factors influencing the inner and outer spinning force was as follows: diameter > nose radius > front angle. Increasing the diameter of the inner roller can improve the spinning stability and forming accuracy of counter-roller spinning. It was beneficial to improve the forming accuracy when the nose radius of the inner roller was slightly larger than that of the outer roller. The front angle of the inner roller has little influence on the spinning forming accuracy.
The Role of Mass Spectrometry in Hepatocellular Carcinoma Biomarker Discovery
Authors: Shen Eric Yi-Liang , U Mei Ran Abellona , Cox I. Jane , Taylor-Robinson Simon D.
Published: October 2023

Hepatocellular carcinoma (HCC) is the main liver malignancy and has a high mortality rate. The discovery of novel biomarkers for early diagnosis, prognosis, and stratification purposes has the potential to alleviate its disease burden. Mass spectrometry (MS) is one of the principal technologies used in metabolomics, with different experimental methods and machine types for different phases of the biomarker discovery process. Here, we review why MS applications are useful for liver cancer, explain the MS technique, and briefly summarise recent findings from metabolomic MS studies on HCC. We also discuss the current challenges and the direction for future research.
Matrix-Assisted Processes in CH4-Doped Ar Ices Irradiated with an Electron Beam
Authors: Bludov Mykhailo , Khyzhniy Ivan , Uyutnov Sergey , Savchenko Elena
Published: October 2023

The relaxation processes induced by exposure of the Ar matrices doped with CH4 (0.1–10%) to an electron beam were studied with a focus on the dynamics of radiolysis products—H atoms, H2 molecules, CH radicals, and energy transfer processes. Three channels of energy transfer to dopant and radiolysis products were discussed, including free charge carriers, free excitons and photons from the “intrinsic source” provided by the emission of the self-trapped excitons. Radiolysis products along with the total yield of desorbing particles were monitored in a correlated manner. Analysis of methane transformation reactions induced by free excitons showed that the CH radical can be considered a marker of the CH3 species. The competition between exciton self-trapping and energy transfer to the dopant and radiolysis products has been demonstrated. A nonlinear concentration behavior of the H atoms in doped Ar matrices has been established. Real-time correlated monitoring of optical emissions (H atom and CH3 radicals), particle ejection, and temperature revealed a nonmonotonic behavior of optical yields with a strong luminescence flash after almost an hour of exposure, which correlated with the explosive pulse of particle ejection and temperature. The connection of this phenomenon with the processes of energy transfer and recombination reactions has been established. It is shown that the delayed explosive ejection of particles is driven by both the recombination of H atoms and CH3 radicals. This occurs after their accumulation to a critical concentration in matrices at a CH4 content C ≥ 1%.
Q Fever in Unexplained Febrile Illness in Northern Algeria
Authors: Ghaoui H. , Achour N. , Saad-Djaballah A. , Belacel S. I. , Bitam I. , Fournier P. E.
Published: October 2023

Our study aimed at assessing the role of Coxiella burnetii in nonspecific febrile illness at the National Center of Infectious Diseases in Algeria, EL-HADI FLICI Hospital. Seventy patients were included and compared to seventy controls without any ongoing infection. Coxiella burnetii infection was investigated using IFA serology and qPCR. Serology was positive in 3 of 70 patients (4.30%), including 1 in whom PCR was also positive (1.42%). All three patients reported frequent contact with ruminants. These results suggest that C. burnetii is an agent of nonspecific febrile illness in Algeria.
Performance of a Novel Real-Time PCR-Based Assay for Rapid Monkeypox Virus Detection in Human Samples
Authors: Liotti Flora Marzia , Marchetti Simona , Falletta Federico , D’Onghia Sara , Sanguinetti Maurizio , Santangelo Rosaria , Posteraro Brunella
Published: October 2023

The ongoing epidemic of mpox, namely human monkeypox virus (MPXV) infection, requires rapid and reliable laboratory diagnosis. We report on the QIAstat-Dx viral vesicular panel PCR assay that allows the detection of (within 75 min) six vesicular disease-causing viruses, including MPXV. We analyzed 168 clinical samples, known to be positive (51 samples) or negative (117 samples) for MPXV clade II, obtained from patients at their mpox diagnosis or follow-up. QIAstat assay results were compared to those of a MPXV-specific reference PCR assay. The QIAstat assay detected MPXV (clade II) in 51 (100%) of 51 samples and did not detect MPXV in 117 (100%) of 117 samples, resulting in a positive or negative agreement of 100% (95% CI, 93.0–100) and 100% (95% CI, 96.8–100), respectively. Of the 20 patients diagnosed with mpox, 18 (90.0%) had at least a vesicular swab and 1 (5.0%) had only an oropharyngeal swab positive for MPXV. At mpox follow-ups, 2 (10.0%) of 20 patients had first-time positive whole blood samples. Thirteen MPXV-negative samples were positive for mpox-mimicking viruses. Our findings show the excellent performance of the QIAstat-Dx assay for MPXV detection in clinical samples. Further studies are needed before considering a large-scale application of the QIAstat-Dx assay.
Melatonin Activation by Human Cytochrome P450 Enzymes: A Comparison between Different Isozymes
Authors: Mokkawes Thirakorn , De Visser Tamar , Cao Yuanxin , De Visser Sam P.
Published: October 2023

Cytochrome P450 enzymes in the human body play a pivotal role in both the biosynthesis and the degradation of the hormone melatonin. Melatonin plays a key role in circadian rhythms in the body, but its concentration is also linked to mood fluctuations as well as emotional well-being. In the present study, we present a computational analysis of the binding and activation of melatonin by various P450 isozymes that are known to yield different products and product distributions. In particular, the P450 isozymes 1A1, 1A2, and 1B1 generally react with melatonin to provide dominant aromatic hydroxylation at the C6-position, whereas the P450 2C19 isozyme mostly provides O-demethylation products. To gain insight into the origin of these product distributions of the P450 isozymes, we performed a comprehensive computational study of P450 2C19 isozymes and compared our work with previous studies on alternative isozymes. The work covers molecular mechanics, molecular dynamics and quantum mechanics approaches. Our work highlights major differences in the size and shape of the substrate binding pocket amongst the different P450 isozymes. Consequently, substrate binding and positioning in the active site varies substantially within the P450 isozymes. Thus, in P450 2C19, the substrate is oriented with its methoxy group pointing towards the heme, and therefore reacts favorably through hydrogen atom abstraction, leading to the production of O-demethylation products. On the other hand, the substrate-binding pockets in P450 1A1, 1A2, and 1B1 are tighter, direct the methoxy group away from the heme, and consequently activate an alternative site and lead to aromatic hydroxylation instead.
Structure–Function Analysis of RBP7910: An Editosome Z-Binding Protein in Trypanosomatids
Authors: Ehlert Curtis , Poorinmohammad Naghmeh , Mohammaei Saba , Zhang Linhua , Salavati Reza
Published: October 2023

RNA editing, a unique post-transcriptional modification, is observed in trypanosomatid parasites as a crucial procedure for the maturation of mitochondrial mRNAs. The editosome protein complex, involving multiple protein components, plays a key role in this process. In Trypanosoma brucei, a putative Z-DNA binding protein known as RBP7910 is associated with the editosome. However, the specific Z-DNA/Z-RNA binding activity and the interacting interface of RBP7910 have yet to be determined. In this study, we conducted a comparative analysis of the binding behavior of RBP7910 with different potential ligands using microscale thermophoresis (MST). Additionally, we generated a 3D model of the protein, revealing potential Z-α and Z-β nucleic acid-binding domains of RBP7910. RBP7910 belongs to the winged-helix–turn–helix (HTH) superfamily of proteins with an α1α2α3β1β2 topology. Finally, using docking techniques, potential interacting surface regions of RBP7910 with notable oligonucleotide ligands were identified. Our findings indicate that RBP7910 exhibits a notable affinity for (CG)n Z-DNA, both in single-stranded and double-stranded forms. Moreover, we observed a broader interacting interface across its Z-α domain when bound to Z-DNA/Z-RNA compared to when bound to non-Z-form nucleic acid ligands.
Crystal Structures and Physicochemical Properties of 3-Chloro-4-hydroxyphenylacetic Acid Salts with Amines
Authors: Ngoma Tchibouanga Remi Rolland , Jacobs Ayesha
Published: October 2023

3-chloro-4-hydroxyphenylacetic acid (CHPAA) is a fungal metabolite. It is a small molecule that is useful in crystal engineering studies due to the functional groups present. Six amines were selected to form salts with CHPAA. Linear derivatives included diethylamine (DEA) and di-N-butylamine (DBM). The aromatic compounds chosen were 2-aminopyridine (A2MP), 2-amino-4-methylpyridine (A24MP), 2-amino-6-methylpyridine (A26MP) and 4-dimethylaminopyridine (DMAP). The salts were characterised using single-crystal X-ray diffraction, thermal analysis, FTIR spectroscopy and Hirshfeld surface analysis. For all the crystal structures, N-H···O and C-H···Cl contacts were present. O-H···O contacts were found in all the crystal structures except for (CHPAA2−)2DEA+, which was also the only structure that displayed a Cl···Cl contact. Furthermore, C-H···O contacts were found in all the crystal structures except for (CHPAA−)(DBM+). The thermal stability trend showed that the DBM salt was more stable than the DEA salt. For the aromatic co-formers, the thermal stability trend showed the following: CHPAA−(DMAP+) > (CHPAA−)(A2MP+)>2CHPAA−2A26MP+>(CHPAA−)(A24MP+).
Sensitive Silver-Enhanced Microplate Apta-Enzyme Assay of Sb3+ Ions in Drinking and Natural Waters
Authors: Komova Nadezhda S. , Serebrennikova Kseniya V. , Berlina Anna N. , Zherdev Anatoly V. , Dzantiev Boris B.
Published: October 2023

The toxic effects of antimony pose risks to human health. Therefore, simple analytical techniques for its widescale monitoring in water sources are in demand. In this study, a sensitive microplate apta-enzyme assay for Sb3+ detection was developed. The biotinylated aptamer A10 was hybridized with its complementary biotinylated oligonucleotide T10 and then immobilized on the surface of polysterene microplate wells. Streptavidin labeled with horseradish peroxidase (HRP) bound to the biotin of a complementary complex and transformed the 3,3′,5,5′-tetramethylbenzidine substrate, generating an optical signal. Sb3+ presenting in the sample bounded to an A10 aptamer, thus releasing T10, preventing streptavidin-HRP binding and, as a result, reducing the optical signal. This effect allowed for the detection of Sb3+ with a working range from 0.09 to 2.3 µg/mL and detection limit of 42 ng/mL. It was established that the presence of Ag+ at the stage of A10/T10 complex formation promoted dehybridization of the aptamer A10 and the formation of the A10/Sb3+ complex. The working range of the Ag+-enhanced microplate apta-enzyme assay for Sb3+ was determined to be 8–135 ng/mL, with a detection limit of 1.9 ng/mL. The proposed enhanced approach demonstrated excellent selectivity against other cations/anions, and its practical applicability was confirmed through an analysis of drinking and spring water samples with recoveries of Sb3+ in the range of 109.0–126.2% and 99.6–106.1%, respectively.
Synthesis of (R)-(6-Methoxyquinolin-4-yl)[(1S,2S,4S,5R)-5-vinylquinuclidin-2-yl]methanol Tetraphenylborate Ion-Pair Complex: Characterization, Antimicrobial, and Computational Study
Authors: Yousef Tarek A. , Alrabiah Haitham , Al-Agamy Mohamed H. , Al-Salahi Rashad , Ali Essam A. , Mostafa Gamal A. E.
Published: October 2023

The (R)-(6-Methoxyquinolin-4-yl)[(1S,2S,4S,5R)-5-vinylquinuclidin-2-yl]methanol (quinine)-tetraphenylborate complex was synthesized by reacting sodium tetraphenyl borate with quinine in deionized water at room temperature through an ion-pair reaction (green chemistry) at room temperature. The solid complex was characterized by several physicochemical methods. The formation of ion-pair complex between bio-active molecules and/or organic molecules is crucial to comprehending the relationships between bioactive molecules and receptor interactions. The complex under study was examined for antimicrobial activity. All theoretical calculations were carried out in vacuum and water using the B3LYP level 6–311G(d,p) levels of theory. The theoretical computation allowed for the prediction and visualization of ionic interactions, which explained the complex’s stability. The results of energy optimization showed that the Q-TPB complex is stable with a negative complexation energy. The obtained geometries showed that the boron (B−) and nitrogen (N+) in piperidine of the two molecules tetraphenylborate and quinine are close to each other, which makes it possible for ions to interact. The modest energy gap between HOMO and LUMO showed that the compound was stable. The computation of the electron transitions of the two models by density functional theory (TD-DFT) in the solvent at the theoretical level B3LYP/6–311G(d,p) allowed for the detection of three UV/visible absorption bands for both models and the discovery of a charge transfer between the host and the guest. The UV absorption, infrared, and H NMR are comparable with the experimental part.
Fabry–Pérot Cavities with Suspended Palladium Membranes on Optical Fibers for Highly Sensitive Hydrogen Sensing
Authors: Xu Feng , Ma Jun , Li Can , Ma Churong , Li Jie , Guan Bai-Ou , Chen Kai
Published: October 2023

Hydrogen (H2) sensors are critical to various applications such as the situation where H2 is used as the clean energy for industry or the indicator for human disease diagnosis. Palladium (Pd) is widely used as the hydrogen sensing material in different types of sensors. Optical fiber H2 sensors are particularly promising due to their compactness and spark-free operation. Here, we report a Fabry–Pérot (FP)-cavity-based H2 sensor that is formed with a freestanding Pd membrane and integrated on a conventional single-mode optical fiber end. The freestanding Pd membrane acts both as the active hydrogen sensing material and as one of the reflective mirrors of the cavity. When the Pd film absorbs H2 to form PdHx, it will be stretched, resulting in a change of the cavity length and thus a shift of the interference spectrum. The H2 concentration can be derived from the amplitude of the wavelength shift. Experimental results showed that H2 sensors based on suspended Pd membranes can achieve a detection sensitivity of about 3.6 pm/ppm and a detection limit of about 3.3 ppm. This highly sensitive detection scheme is expected to find applications for sensing low-concentration H2.
Garcinia mangostana L. Pericarp Extract and Its Active Compound α-Mangostin as Potential Inhibitors of Immune Checkpoint Programmed Death Ligand-1
Authors: Naing Sandar , Sandech Nichawadee , Maiuthed Arnatchai , Chongruchiroj Sumet , Pratuangdejkul Jaturong , Lomarat Pattamapan
Published: October 2023

α-Mangostin, a major xanthone found in mangosteen (Garcinia mangostana L., Family Clusiaceae) pericarp, has been shown to exhibit anticancer effects through multiple mechanisms of action. However, its effects on immune checkpoint programmed death ligand-1 (PD-L1) have not been studied. This study investigated the effects of mangosteen pericarp extract and its active compound α-mangostin on PD-L1 by in vitro and in silico analyses. HPLC analysis showed that α-mangostin contained about 30% w/w of crude ethanol extract of mangosteen pericarp. In vitro experiments in MDA-MB-231 triple-negative breast cancer cells showed that α-mangostin and the ethanol extract significantly inhibit PD-L1 expression when treated for 72 h with 10 µM or 10 µg/mL, respectively, and partially inhibit glycosylation of PD-L1 when compared to untreated controls. In silico analysis revealed that α-mangostin effectively binds inside PD-L1 dimer pockets and that the complex was stable throughout the 100 ns simulation, suggesting that α-mangostin stabilized the dimer form that could potentially lead to degradation of PD-L1. The ADMET prediction showed that α-mangostin is lipophilic and has high plasma protein binding, suggesting its greater distribution to tissues and its ability to penetrate adipose tissue such as breast cancer. These findings suggest that α-mangostin-rich mangosteen pericarp extract could potentially be applied as a functional ingredient for cancer chemoprevention.
A Novel and Quantitative Detection Assay (effluxR) for Identifying Efflux-Associated Resistance Genes Using Multiplex Digital PCR in Clinical Isolates of Pseudomonas aeruginosa
Authors: Rattanachak Nontaporn , Weawsiangsang Sattaporn , Baldock Robert A. , Jaifoo Theerasak , Jongjitvimol Touchkanin , Jongjitwimol Jirapas
Published: October 2023

The rise of multidrug resistance of Pseudomonas aeruginosa highlights an increased need for selective and precise antimicrobial treatment. Drug efflux pumps are one of the major mechanisms of antimicrobial resistance found in many bacteria, including P. aeruginosa. Detection of efflux genes using a polymerase chain reaction (PCR)-based system would enable resistance detection and aid clinical decision making. Therefore, we aimed to develop and optimize a novel method herein referred to as “effluxR detection assay” using multiplex digital PCR (mdPCR) for detection of mex efflux pump genes in P. aeruginosa strains. The annealing/extension temperatures and gDNA concentrations were optimized to amplify mexB, mexD, and mexY using the multiplex quantitative PCR (mqPCR) system. We established the optimal mqPCR conditions for the assay (Ta of 59 °C with gDNA concentrations at or above 0.5 ng/µL). Using these conditions, we were able to successfully detect the presence of these genes in a quantity-dependent manner. The limit of detection for mex genes using the effluxR detection assay with mdPCR was 0.001 ng/µL (7.04–34.81 copies/µL). Moreover, using blind sample testing, we show that effluxR detection assay had 100% sensitivity and specificity for detecting mex genes in P. aeruginosa. In conclusion, the effluxR detection assay, using mdPCR, is able to identify the presence of multiple mex genes in P. aeruginosa that may aid clinical laboratory decisions and further epidemiological studies.
Development of Solid Lipid Nanoparticles for Controlled Amiodarone Delivery
Authors: Creteanu Andreea , Lisa Gabriela , Vasile Cornelia , Popescu Maria-Cristina , Spac Adrian Florin , Tantaru Gladiola
Published: October 2023

In various drug delivery systems, solid lipid nanoparticles are dominantly lipid-based nanocarriers. Amiodarone hydrochloride is an antiarrhythmic agent used to treat severe rhythm disturbances. It has variable and hard-to-predict absorption in the gastrointestinal tract because of its low solubility and high permeability. The aims of this study were to improve its solubility by encapsulating amiodarone into solid lipid nanoparticles using two excipients—Compritol® 888 ATO (pellets) (C888) as a lipid matrix and Transcutol® (T) as a surfactant. Six types of amiodarone-loaded solid lipid nanoparticles (AMD-SLNs) were obtained using a hot homogenization technique followed by ultrasonication with varying sonication parameters. AMD-SLNs were characterized by their size distribution, polydispersity index, zeta potential, entrapment efficiency, and drug loading. Based on the initial evaluation of the entrapment efficiency, only three solid lipid nanoparticle formulations (P1, P3, and P5) were further tested. They were evaluated through scanning electron microscopy, Fourier-transform infrared spectrometry, near-infrared spectrometry, thermogravimetry, differential scanning calorimetry, and in vitro dissolution tests. The P5 formulation showed optimum pharmaco-technical properties, and it had the greatest potential to be used in oral pharmaceutical products for the controlled delivery of amiodarone.
Facile and Low-Cost Fabrication of SiO2-Covered Au Nanoislands for Combined Plasmonic Enhanced Fluorescence Microscopy and SERS
Authors: Vidal Alejandro , Molina-Prados Sergio , Cros Ana , Garro Núria , Pérez-Martínez Manuel , Álvaro Raquel , Mata Gadea , Megías Diego , Postigo Pablo A.
Published: October 2023

An easy and low-cost way to fabricate monometallic Au nanoislands for plasmonic enhanced spectroscopy is presented. The method is based on direct thermal evaporation of Au on glass substrates to form nanoislands, with thicknesses between 2 and 15 nm, which are subsequently covered by a thin layer of silicon dioxide. We have used HR-SEM and AFM to characterize the nanoislands, and their optical transmission reveals strong plasmon resonances in the visible. The plasmonic performance of the fabricated substrates has been tested in fluorescence and Raman scattering measurements of two probe materials. Enhancement factors up to 1.8 and 9×104 are reported for confocal fluorescence and Raman microscopies, respectively, which are comparable to others obtained by more elaborated fabrication procedures.
Survey for Soil Sensing with IOT and Traditional Systems
Authors: Wang Juexing , Zhang Xiao , Xiao Li , Li Tianxing
Published: October 2023

Smart Agriculture has gained significant attention in recent years due to its benefits for both humans and the environment. However, the high costs associated with commercial devices have prevented some agricultural lands from reaping the advantages of technological advancements. Traditional methods, such as reflectance spectroscopy, offer reliable and repeatable solutions for soil property sensing, but the high costs and redundancy of preprocessing steps limit their on-site applications in real-world scenarios. Recently, RF-based soil sensing systems have opened a new dimension in soil property analysis using IoT-based systems. These systems are not only portable, but also significantly cheaper than traditional methods. In this paper, we carry out a comprehensive review of state-of-the-art soil property sensing, divided into four areas. First, we delve into the fundamental knowledge and studies of reflectance-spectroscopy-based soil sensing, also known as traditional methods. Secondly, we introduce some RF-based IoT soil sensing systems employing a variety of signal types. In the third segment, we introduce the details of sample pretreatment, inference methods, and evaluation metrics. Finally, after analyzing the strengths and weaknesses of the current work, we discuss potential future aspects of soil property sensing.
Colon-Targeted Delivery of Indole Acetic Acid Helps Regulate Gut Motility by Activating the AHR Signaling Pathway
Authors: Chen Ying , Pan Ruili , Mei Liya , Tian Peijun , Wang Linlin , Zhao Jianxin , Chen Wei , Wang Gang
Published: October 2023

Intestinal peristalsis is vital for gastrointestinal physiology and host homeostasis and is frequently dysregulated in intestinal disorders. Gut microbiota can regulate gut motility, especially through the tryptophan metabolism pathway. However, the role of indoles as microbial tryptophan metabolites in colonic function requires further exploration. Here, we show that the delivery of indole acetic acid (IAA) targeting the colon can improve gut motility by activating the aryl hydrocarbon receptor (AHR). To achieve colon-targeted delivery, Eudragit S-100 (ES) and chitosan (CS) were used as drug carriers. After optimisation, IAA-loaded ES-coated CS nanoparticles exhibited an encapsulation efficiency of 83% and a drug-loading capacity of 16%. These nanoparticles exhibited pH-dependent characteristics and remained stable in acidic conditions and the upper intestine. In simulated intestinal fluid (pH 7.4) and colonic lumen, considerable amounts of IAA were released after approximately 4 h. Compared with free IAA, the nanoparticles exerted enhanced therapeutic effects on gut movement disorders induced by loperamide. The efficacy of IAA treatment was attributable to the activation of the AHR signalling pathway and increased levels of AHR agonists. Furthermore, the oral administration of IAA-loaded nanoparticles promoted serotonin secretion and maintained the intestinal barrier function. The experimental outcomes demonstrate the efficiency of the proposed colon-specific delivery system and highlight the role of IAA, produced by gut microbiota metabolism, in regulating gut peristalsis through AHR activation.
Diet Quality, Cardiometabolic Risk and Diabetes
Authors: Della Pepa Giuseppe
Published: October 2023

The alarming rise in obesity worldwide is a dramatic public health concern [...]
Artificial Intelligence Applications to Public Health Nutrition
Authors: An Ruopeng , Wang Xiaoxin
Published: October 2023

Public health nutrition occupies a paramount position in the overarching domains of health promotion and disease prevention, setting itself apart from nutritional investigations concentrated at the individual level [...]
Preeclampsia and Its Impact on Human Milk Activin A Concentration
Authors: Coscia Alessandra , Riboldi Lorenzo , Spada Elena , Bertino Enrico , Sottemano Stefano , Barbagallo Ignazio , Livolti Giovanni , Galvano Fabio , Gazzolo Diego , Peila Chiara
Published: October 2023

Background: It is known that preeclampsia affects lactogenesis. However, data on the effects of this pathology on human milk neurobiomarker composition are not available. The aim of this study is to investigate the effects of this gestational pathology on activin A levels, a neurobiomarker known to play an important role in the development and protection of the central nervous system. Methods: The women recruited were divided in two different study groups: preeclamptic or normotensive women. All the human milk samples were collected using the same procedure. Activin A was quantified using an Enzyme-linked immunosorbent assay (ELISA) test. To investigate the effect of preeclampsia on the activin A concentration in the three lactation phases, a mixed linear model with a unistructural covariance structure, with the mother as the random effect, and fixed effects were performed. Results: Activin A was detected in all samples. There were no significant differences between preeclamptic and normotensive women. The only significant effect is related to the lactation phase: the difference between colostrum and mature milk (p < 0.01) was significant. In conclusion, these results allow us to affirm that breast milk’s beneficial properties are maintained even if preeclampsia occurs.
Absorption Spectra of Protonated Corroles: Two Distinct Patterns Due to Peripheral Substitution Architecture
Authors: Gladkov Lev L. , Klenitsky Dmitry V. , Kruk Mikalai M.
Published: October 2023

The origin of individual features in the ground state absorption spectra of two protonated corroles differing in the architecture of peripheral substitution (either Cm-aryl or Cb-alkyl) have been studied in detail with the ground state absorption spectroscopy and density functional theory calculations. The geometry optimization, molecular orbitals and absorption spectra calculation have been carried out. It was found that protonation leads to the saddle type macrocycle conformation in contrast with the wave type conformation known for the parent-free base corroles. The mean plane deviation parameter Δ23 for the macrocycle, pyrrole tilting angles and the degree of pyramidalization λ2 of all four pyrrole nitrogens was found to depend on the peripheral substitution architecture. Macrocycle conformation of the protonated forms has distinct asymmetrical features which are reflected by the sets of values of the tilting angles and values of pyramidalization degree. The pair of pyrroles B and C has smaller tilting angles and higher pyramidalization degree values, whereas the opposite trend was found for the pair of pyrroles A and D. Electronic effects and structural differences induced by substitution lead to the pronounced shifts of the molecular orbitals. In the Cb-alkylated corrole, almost-degenerated HOMO and HOMO-1 molecular orbitals lead to enhancement of the configuration interaction. As a result, the Qx transition oscillator strength goes down, becoming comparable to that of the Qy one. A large HOMO-HUMO-1gap in the Cm-aryl corrole minimizes the configuration interaction, giving rise to Qx band domination in the visible range spectrum.
Distribution of Opportunistic Pathogens in People Living with HIV at a University Hospital in Istanbul over a One-Year Treatment Period and Its Association with CD4 T Cell Counts
Authors: Kirkoyun Uysal Hayriye , Koksal Muammer Osman , Sarsar Kutay , Soguksu Pinar , Erkose Genc Gonca , Yapar Gizem , Ozdemir Evrim , Onel Mustafa , Mese Sevim , Demirci Mehmet , Erturan Zayre , Yurtseven Eray , Eraksoy Omer Haluk , Agacfidan Ali
Published: October 2023

Among sexually transmitted diseases, HIV causes very serious clinical manifestations that can lead to death. As a result, millions of people have to live with this problem that threatens their health. The virus attacks the immune system of the host, especially CD4+ T lymphocytes, causing the suppression of the immune system. CD4, CD8 counts, and HIV RNA viral loads are monitored in HIV-infected patients with antiretroviral treatment, and CD4 counts play an important role in determining the effectiveness of the treatment. Despite the advances in treatment in the present day, opportunistic infections are the main cause of morbidity and mortality in these patients, and the evaluation of immunological parameters is valuable for the prognosis of the disease in this process. In the present study, the purpose was to investigate the opportunistic infections faced by naive HIV-positive patients who applied to our laboratory and were diagnosed between 2019 and 2022 during their one-year treatment period, and the correlation of the immunological parameters was also evaluated retrospectively using the hospital automation system and laboratory data. A total of 107 opportunistic causative microorganisms were identified in 87 of the 230 HIV-positive patients over one year. T. pallidum was detected in 43 (18.6%) of these patients, Cytomegalovirus (CMV) in 32 (13.9%), Epstein–Barr virus (EBV) in 9 (3.9%), Hepatitis B virus (HBV) in 10 (4.3%), C. albicans in 7 (3%), M. tuberculosis in 3 (1.3%), Hepatitis C virus (HCV) in 2 (0.8%), and C. glabrata in 1 (0.4%) patient. Although mono-agent co-infections were determined in 69 of 87 people living with HIV, two-agent co-infections were detected in 16 HIV patients, and three-agent co-infections were identified in two HIV patients. Considering the correlation between the CD4/CD8 ratio and infection positivity, a moderate negative correlation was determined with HIV RNA viral load and CMV infection. The CD4/CD8 ratio had a low negative correlation with EBV and C. albicans infections. It was also found that the follow-up of HIV RNA load in the diagnosis of T. pallidum, CMV, EBV, and C. albicans may be meaningful. Opportunistic infections mainly affect immunosuppressed patients and can be prevented with effective treatment. Although it is already known that HIV patients may face different infections during their treatment, it was concluded that more attention should be paid to T. pallidum, CMV, EBV, and C. albicans agents. These infections should be routinely monitored with HIV viral load and the CD4/CD8 ratio.
Comment on Dao et al. Retrospective Analysis of Real-World Data for the Treatment of Obstructive Sleep Apnea with Slow Maxillary Expansion Using a Unique Expansion Dental Appliance (DNA). Pathophysiology 2023, 30, 199–208
Authors: Singh G. Dave
Published: October 2023

I found the recent article by Dao et al [...]
Recent Progress in Diatom Biosilica: A Natural Nanoporous Silica Material as Sustained Release Carrier
Authors: Lim Hayeon , Seo Yoseph , Kwon Daeryul , Kang Sunggu , Yu Jiyun , Park Hyunjun , Lee Sang Deuk , Lee Taek
Published: October 2023

A drug delivery system (DDS) is a useful technology that efficiently delivers a target drug to a patient’s specific diseased tissue with minimal side effects. DDS is a convergence of several areas of study, comprising pharmacy, medicine, biotechnology, and chemistry fields. In the traditional pharmacological concept, developing drugs for disease treatment has been the primary research field of pharmacology. The significance of DDS in delivering drugs with optimal formulation to target areas to increase bioavailability and minimize side effects has been recently highlighted. In addition, since the burst release found in various DDS platforms can reduce drug delivery efficiency due to unpredictable drug loss, many recent DDS studies have focused on developing carriers with a sustained release. Among various drug carriers, mesoporous silica DDS (MS-DDS) is applied to various drug administration routes, based on its sustained releases, nanosized porous structures, and excellent solubility for poorly soluble drugs. However, the synthesized MS-DDS has caused complications such as toxicity in the body, long-term accumulation, and poor excretion ability owing to acid treatment-centered manufacturing methods. Therefore, biosilica obtained from diatoms, as a natural MS-DDS, has recently emerged as an alternative to synthesized MS-DDS. This natural silica carrier is an optimal DDS platform because culturing diatoms is easy, and the silica can be separated from diatoms using a simple treatment. In this review, we discuss the manufacturing methods and applications to various disease models based on the advantages of biosilica.
Selection of Excipients for the Preparation of Vancomycin-Loaded Poly(D,L-lactide-co-glycolide) Microparticles with Extended Release by Emulsion Spray Drying
Authors: Jurić Simčić Ana , Erak Iva , Cetina Čižmek Biserka , Hafner Anita , Filipović-Grčić Jelena
Published: October 2023

The aim of this study was to relate the composition of the W/O emulsion used as a starting fluid in the spray-drying process to the quality of the dry polymer particles obtained in terms of physical–chemical properties, compatibility and drug release performance. Four W/O emulsions containing vancomycin hydrochloride (VAN), an encapsulating PLGA polymer and Poloxamer® 407, chitosan and/or sorbitan monooleate as stabilisers were spray-dried using an ultrasonic atomising nozzle. The microparticles obtained were micron-sized, with a volume mean diameter between 43.2 ± 0.3 and 64.0 ± 12.6 µm, and spherical with a mostly smooth, non-porous surface and with high drug loading (between 14.5 ± 0.6 and 17.1 ± 1.9% w/w). All formulations showed a prolonged and biphasic VAN release profile, with diffusion being the primary release mechanism. Microparticles prepared from the emulsions with Poloxamer® 407 and sorbitan monooleate released VAN rapidly and completely within one day. The release of VAN from microparticles prepared from the emulsion without additives or with chitosan in the inner aqueous phase was significantly decreased; after four days, a cumulative release of 65% and 61%, respectively, was achieved. Microparticles with encapsulated chitosan had the largest mean particle diameter and the slowest release of VAN.
The Path from Nasal Tissue to Nasal Mucosa on Chip: Part 2—Advanced Microfluidic Nasal In Vitro Model for Drug Absorption Testing
Authors: Koch Eugen Viktor , Bendas Sebastian , Nehlsen Kristina , May Tobias , Reichl Stephan , Dietzel Andreas
Published: October 2023

The nasal mucosa, being accessible and highly vascularized, opens up new opportunities for the systemic administration of drugs. However, there are several protective functions like the mucociliary clearance, a physiological barrier which represents is a difficult obstacle for drug candidates to overcome. For this reason, effective testing procedures are required in the preclinical phase of pharmaceutical development. Based on a recently reported immortalized porcine nasal epithelial cell line, we developed a test platform based on a tissue-compatible microfluidic chip. In this study, a biomimetic glass chip, which was equipped with a controlled bidirectional airflow to induce a physiologically relevant wall shear stress on the epithelial cell layer, was microfabricated. By developing a membrane transfer technique, the epithelial cell layer could be pre-cultivated in a static holder prior to cultivation in a microfluidic environment. The dynamic cultivation within the chip showed a homogenous distribution of the mucus film on top of the cell layer and a significant increase in cilia formation compared to the static cultivation condition. In addition, the recording of the ciliary transport mechanism by microparticle image velocimetry was successful. Using FITC-dextran 4000 as an example, it was shown that this nasal mucosa on a chip is suitable for permeation studies. The obtained permeation coefficient was in the range of values determined by means of other established in vitro and in vivo models. This novel nasal mucosa on chip could, in future, be automated and used as a substitute for animal testing.
Acousto-Optic Q-Switched Ho:YLF Ring Laser Based on Anti-Misalignment Resonant Cavity
Authors: Wang Yunpeng , Zhang Dongming , Yan Dong , Dai Tongyu , Ju Youlun
Published: October 2023

An acousto-optic Q-switching Ho:YLF ring oscillator at 2066.33 nm with two anti-misalignment corner cube reflectors (CCRs) pumped by a 1940 nm thulium-doped fiber laser is demonstrated. The depolarization effect of the CCR is expressed in the form of equivalent transmission, and the transmission from two output directions of the oscillator is changing synchronously and periodically as the waveplate angle changes. In the experiment, under the pump power of 21.76 W, the optimum bidirectional energy of 3.13 mJ for a pulse duration of 122 ns at a repetition rate of 50 Hz is realized. The pulse energy reduction percentage at 50 Hz is 8% by changing the horizontal drift angle of one of the CCRs to 4.5°. The beam quality factor M2 is calculated to be about 1.10, revealing that the Ho laser is working in a fundamental transverse mode.
Control of Spatiotemporal Localization of Infrared Pulses in Gas-Filled Capillaries Using Weak Ultraviolet Pulses
Authors: Han Lize , Gao Xiaohui
Published: October 2023

Manipulation of intense pulse propagation in gas-filled capillaries is desirable for various high-field applications. Tuning the parameters of the driving laser pulse and the working gas is the conventional approach, and it provides limited capability of control. Here we demonstrate through numerical simulations a practical scheme to control the propagation of intense pulses. A weak ultraviolet pulse is launched into a capillary with a negative delay with respect to a main infrared pulse. The pulses begin to temporally overlap due to dispersion. As the main pulse self-compresses, the control pulse is strongly red-shifted due to cross-phase modulation. The frequency shifts of the two pulses mitigate pulse walk-off and allow an efficient coupling, substantially extending the effective interaction length. This interesting phenomenon may benefit applications such as high-order harmonic generation.
Population Structure and Genetic Diversity of the 175 Soybean Breeding Lines and Varieties Cultivated in West Siberia and Other Regions of Russia
Authors: Potapova Nadezhda A. , Zlobin Alexander S. , Perfil’ev Roman N. , Vasiliev Gennady V. , Salina Elena A. , Tsepilov Yakov A.
Published: October 2023

Soybean is a leguminous plant cultivated in many countries and is considered important in the food industry due to the high levels of oil and protein content in the beans. The high demand for soybeans and its products in the industry requires the expansion of cultivation areas. Despite climatic restrictions, West Siberia is gradually expanding its area of soybean cultivation. In this study, we present the first analysis of the population structure and genetic diversity of the 175 soybean Glycine max breeding lines and varieties cultivated in West Siberia (103 accessions) and other regions of Russia (72 accessions), and we compare them with the cultivated soybean varieties from other geographical locations. Principal component analysis revealed several genetic clusters with different levels of genetic heterogeneity. Studied accessions are genetically similar to varieties from China, Japan, and the USA and are genetically distant to varieties from South Korea. Admixture analysis revealed four ancestry groups based on genetic ancestry and geographical origin, which are consistent with the regions of cultivation and origin of accessions and correspond to the principal component analysis result. Population statistics, including nucleotide diversity, Tajima’s D, and linkage disequilibrium, are comparatively similar to those observed for studied accessions of a different origin. This study provides essential population and genetic information about the unique collection of breeding lines and varieties cultivated in West Siberia and other Russian regions to foster further evolutionary, genome-wide associations and functional breeding studies.
Artemisia santonicum L. and Artemisia lerchiana Web. Essential Oils and Exudates as Sources of Compounds with Pesticidal Action
Authors: Nikolova Milena , Lyubenova Aneta , Yankova-Tsvetkova Elina , Georgiev Borislav , Berkov Strahil , Aneva Ina , Trendafilova Antoaneta
Published: October 2023

The application of natural products for pest control is important in modern farming. In the present study, Artemisia santonicum L. and Artemisia lerchiana Weber essential oil and exudate profiles were determined, and their potential as inhibitors of seed germination, acetylcholinesterase, and phytopathogenic mycelium growth were evaluated. Essential oils (EO) were obtained via hydrodistillation and exudates (AE) by washing aerial parts of the species with acetone. EO and AE’s composition was identified using GC/MS. Eucalyptol (1,8-cineole) and camphor were found to be the main components of A. lerchiana EO, while β-pinene, trans-pinocarveol, α-pinene, α-terpineol, and spathulenol were established as major compounds of A. santonicum EO. Strong inhibition on Lolium perenne seed germination was found at 2 µL/mL and 5 mg/mL using aqueous solutions of EO and AE, respectively. An inhibitory effect on acetylcholinesterase was established, with an IC50 value of 64.42 and 14.60 μg/mL for EO and 0.961, >1 mg/mL for the AE of A. lerchiana and A. santonicum, respectively. The low inhibition on the mycelium growth of studied phytopathogenic fungi was established by applying 2 µL of EO and 15 µL of 100 mg/mL of AE, with the exception of A. lerchiana AE against Botrytis cinerea. These results show that the studied EO and AE exhibited strong phytotoxic and AChE inhibitory activities, providing new data for these species.