bluuebunny/crossref_metadata_2025 huggingface dataset:
Resilient Security Framework Using TNN and Blockchain for IoMT
Authors: Alsemmeari Rayan A. , Dahab Mohamed Yehia , Alsulami Abdulaziz A. , Alturki Badraddin , Algarni Sultan
Published: May 2023

The growth of the Internet of Things (IoT) devices in the healthcare sector enables the new era of the Internet of Medical Things (IoMT). However, IoT devices are susceptible to various cybersecurity attacks and threats, which lead to negative consequences. Cyberattacks can damage not just the IoMT devices in use but also human life. Currently, several security solutions have been proposed to enhance the security of the IoMT, employing machine learning (ML) and blockchain. ML can be used to develop detection and classification methods to identify cyberattacks targeting IoMT devices in the healthcare sector. Furthermore, blockchain technology enables a decentralized approach to the healthcare system, eliminating some disadvantages of a centralized system, such as a single point of failure. This paper proposes a resilient security framework integrating a Tri-layered Neural Network (TNN) and blockchain technology in the healthcare domain. The TNN detects malicious data measured by medical sensors to find fraudulent data. As a result, cyberattacks are detected and discarded from the IoMT system before data is processed at the fog layer. Additionally, a blockchain network is used in the fog layer to ensure that the data is not altered, enhancing the integrity and privacy of the medical data. The experimental results show that the TNN and blockchain models produce the expected result. Furthermore, the accuracy of the TNN model reached 99.99% based on the F1-score accuracy metric.
Bibliometric Analysis of Automated Assessment in Programming Education: A Deeper Insight into Feedback
Authors: Paiva José Carlos , Figueira Álvaro , Leal José Paulo
Published: May 2023

Learning to program requires diligent practice and creates room for discovery, trial and error, debugging, and concept mapping. Learners must walk this long road themselves, supported by appropriate and timely feedback. Providing such feedback in programming exercises is not a humanly feasible task. Therefore, the early and steadily growing interest of computer science educators in the automated assessment of programming exercises is not surprising. The automated assessment of programming assignments has been an active area of research for over a century, and interest in it continues to grow as it adapts to new developments in computer science and the resulting changes in educational requirements. It is therefore of paramount importance to understand the work that has been performed, who has performed it, its evolution over time, the relationships between publications, its hot topics, and open problems, among others. This paper presents a bibliometric study of the field, with a particular focus on the issue of automatic feedback generation, using literature data from the Web of Science Core Collection. It includes a descriptive analysis using various bibliometric measures and data visualizations on authors, affiliations, citations, and topics. In addition, we performed a complementary analysis focusing only on the subset of publications on the specific topic of automatic feedback generation. The results are highlighted and discussed.
Decarbonisation of Geographical Islands and the Feasibility of Green Hydrogen Production Using Excess Electricity
Authors: Madi Hossein , Lytvynenko Dmytro , Schildhauer Tilman , Jansohn Peter
Published: May 2023

Islands face limitations in producing and transporting energy due to their geographical constraints. To address this issue, the ROBINSON project, funded by the EU, aims to create a flexible, self-sufficient, and environmentally friendly energy system that can be used on isolated islands. The feasibility of renewable electrification and heating system decarbonization of Eigerøy in Norway is described in this article. A mixed-integer linear programming framework was used for modelling. The optimization method is designed to be versatile and adaptable to suit individual scenarios, with a flexible and modular formulation that can accommodate boundary conditions specific to each case. Onshore and offshore wind farms and utility-scale photovoltaic (PV) were considered to generate renewable electricity. Each option was found to be feasible under certain conditions. The heating system, composed of a biomass gasifier, a combined heat and power system with a gas boiler as backup unit, was also analyzed. Parameters were identified in which the combination of all three thermal units represented the best system option. In addition, the possibility of green hydrogen production based on the excess electricity from each scenario was evaluated.
Short-Term Load Forecasting of Electricity Demand for the Residential Sector Based on Modelling Techniques: A Systematic Review
Authors: Rodrigues Filipe , Cardeira Carlos , Calado João M. F. , Melicio Rui
Published: May 2023

In this paper, a systematic literature review is presented, through a survey of the main digital databases, regarding modelling methods for Short-Term Load Forecasting (STLF) for hourly electricity demand for residential electricity and to realize the performance evolution and impact of Artificial Intelligence (AI) in STLF. With these specific objectives, a conceptual framework on the subject was developed, along with a systematic review of the literature based on scientific publications with high impact and a bibliometric study directed towards the scientific production of AI and STLF. The review of research articles over a 10-year period, which took place between 2012 and 2022, used the Preferred Reporting Items for Systematic and Meta-Analyses (PRISMA) method. This research resulted in more than 300 articles, available in four databases: Web of Science, IEEE Xplore, Scopus, and Science Direct. The research was organized around three central themes, which were defined through the following keywords: STLF, Electricity, and Residential, along with their corresponding synonyms. In total, 334 research articles were analyzed, and the year of publication, journal, author, geography by continent and country, and the area of application were identified. Of the 335 documents found in the initial research and after applying the inclusion/exclusion criteria, which allowed delimiting the subject addressed in the topics of interest for analysis, 38 (thirty-eight) documents were in English (26 journal articles and 12 conference papers). The results point to a diversity of modelling techniques and associated algorithms. The corresponding performance was measured with different metrics and, therefore, cannot be compared directly. Hence, it is desirable to have a unified dataset, together with a set of benchmarks with well-defined metrics for a clear comparison of all the modelling techniques and the corresponding algorithms.
Carbon Dioxide Separation Technologies: Applicable to Net Zero
Authors: Rath Gourav Kumar , Pandey Gaurav , Singh Sakshi , Molokitina Nadezhda , Kumar Asheesh , Joshi Sanket , Chauhan Geetanjali
Published: May 2023

Carbon dioxide (CO2) emissions from burning fossil fuels play a crucial role in global warming/climate change. The effective removal of CO2 from the point sources or atmosphere (CO2 capture), its conversion to value-added products (CO2 utilization), and long-term geological storage, or CO2 sequestration, has captured the attention of several researchers and policymakers. This review paper illustrates all kinds of CO2 capture/separation processes and the challenges faced in deploying these technologies. This review described the research efforts put forth in gas separation technologies. Recent advances in the existing gas separation technologies have been highlighted, and future directives for commercial deployment have also been outlined.
Inertia Identification and Analysis for High-Power-Electronic-Penetrated Power System Based on Measurement Data
Authors: Xu Zhentao , Ma Junjie , Gao Yousong , Li Yong , Yu Haifeng , Wang Lu
Published: May 2023

With the gradual increases in the use of wind power and photovoltaic generation, the penetration rate of power electronics has increased in recent years. The inertia characteristics of power-electronic-based power sources are different from those of synchronous generators, making the evaluation of inertia difficult. In this paper, the inertia characteristics of power-electronic-based power sources are analyzed. A measurement-based inertia identification method for power-electronic-based power sources, as well as for high-power-electronic-penetrated power systems, is proposed by fitting the frequency and power data. The inertia characteristics of different control strategies and corresponding control parameters are discussed in a case study. It was proven that the inertia provided by power-electronic-based power sources can be much higher than that provided by a synchronous generator of the same capacity. It was also proven that the inertia provided by power-electronic-based power sources is not a constant value, but changes along with the output power of the sources.
A Bridge-Level Junction Temperature Estimation Method for SiC MOSFETs Combining Transient Voltage and Current Peaks
Authors: Wu Shuo , Sun Pengju , Huang Xu , Li Kaiwei
Published: May 2023

Accurate measurement of the junction temperature Tj is crucial for ensuring safe operation and evaluating the performance of silicon carbide (SiC) metal–oxide–semiconductor field-effect transistors (MOSFETs). In this paper, the junction temperature of a half-bridge is monitored by combining transient voltage and current peaks across the lower device. Both theoretical analysis and switch tests validate that the peak induced voltage and peak reversed current during the commutation transient exhibit a linear relationship with the temperature of the corresponding devices. The impact of load current and bus voltage on the measurement results is also investigated. Moreover, the effectiveness of the proposed method is confirmed by carrying out tests at different temperatures, and the feasibility of online implementation is discussed. The experimental results show that the proposed method has high linearity and sensitivity and can simultaneously provide temperature information for both devices of a half-bridge.
Energy Efficiency of AGV-Drone Joint In-Plant Supply of Production Lines
Authors: Bányai Tamás
Published: May 2023

Energy efficiency plays an increasingly important role not only in supply chains, but also in in-plant supply systems. Manufacturing companies are increasingly using energy-efficient material handling equipment to solve their in-plant material handling tasks. A new example of this effort is the use of drones for in-plant transportation of small components. Within the frame of this article, a new AGV-drone joint in-plant supply model is described. The joint service of AGV-based milkrun trolleys and drones makes it possible to optimize the in-plant supply in production lines. This article discusses the mathematical description of AGV-drone joint in-plant supply solutions. The numerical analysis of the different AGV-drone joint in-plant supply solutions shows that this new approach can lead to an energy consumption reduction of about 30%, which also has a significant impact on GHG emission.
A CFD Modelling Approach for the Operation Analysis of an Exhaust Backpressure Valve Used in a Euro 6 Diesel Engine
Authors: Martos Francisco J. , Soriano José A. , Braic Andrei , Fernández-Yáñez Pablo , Armas Octavio
Published: May 2023

Harvesting residual thermal energy from exhaust gases with thermoelectric generators is one of the paths that are currently being explored to achieve more sustainable and environmentally friendly means of transport. In some cases, thermoelectric generators are installed in a by-pass configuration to regulate the mass flow entering the thermoelectric generator. Some manufacturers are using throttle valves with electromechanical actuators and electronic control in the exhaust pipe to improve techniques for active control of pollutant emissions in reciprocating internal combustion engines, such as the exhaust gas recirculation. The above-mentioned circumstances have motivated the approach of this work: computational fluid dynamics (CFD) modelling of the operation of a throttle valve used for establishing adequate exhaust backpressure conditions to achieve the low pressure exhaust gas recirculation in Euro 6 engines. The aim of this model is to understand the flow control process with these types of valves in order to incorporate them in an exhaust system that will include two thermoelectric generators used to convert residual thermal energy into electrical energy. This work presents a computational model of the flow through the throttle valve under different temperatures and mass flow rates of the exhaust gas with different closing positions. For all cases, the values of the pressure drop were obtained. In all cases studied, the level of agreement between the modelled and experimental results exceeds 90%. The developed model has helped to propose a correlation to estimate the mass flow rate of exhaust gas from easily measurable quantities.
Influence of Physical and Mechanical Parameters on Cavitation Erosion and Antifouling Behaviour of Multilayer Silica-Based Hybrid Sol–Gel Coatings on Aluminium Alloys
Authors: Hegde Manasa , Mroczkowska Marta , Mohan Joseph , Neves Adriana Cunha , Kavanagh Yvonne , Duffy Brendan , Tobin Edmond F.
Published: May 2023

Sol–gel coatings can provide anti-fouling and erosion resistance while being safe to use in the marine environment. MAPTMS/ZPO multilayer coatings deposited on the AA2024-T3 aluminium surface using the dip-coating method at three different thicknesses (2, 4, and 6 µm) are investigated in this work. The coatings are characterised in terms of physical and mechanical properties, and these properties are investigated in comparison to previously obtained cavitation erosion resistance levels of the coatings. Additionally, the efficiency of the coatings against biofouling was assessed using Phaeodactylum tricornutum, a marine diatom. The influence of the formation of organic–inorganic hybrid materials (OIHMs) from the prepared sols on the physical and mechanical properties of the coatings were analysed. A variety of techniques, including attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR), water contact angle (WCA) measurements, pencil hardness testing, cross-cut adhesion testing, a roughness profilometer, and nano-indentation, were performed on the bare and coated substrates. The results indicated that the thickness, hydrophobicity, and adherence of the coatings are strongly affected by the roughness. The elastic strain failure (H/E) and resistance to plastic deformation (H3/E2) coefficients were higher than those of the bare substrate before and after the cavitation erosion test, indicating that the coating had a higher ability to withstand deformation in comparison to the substrate alone. Furthermore, the microscopic analysis of a marine diatom, Phaeodactylum tricornutum, revealed that coated surfaces exhibited a decreased rate of bacterial adhesion and biofilm formation. The data show that sol–gel formed coatings outperform uncoated AA2024-T3 in terms of hardness, elastic strain, plastic deformation, and biofouling resistance. These characteristics are attributed to the coatings’ mechanical and adhesive capabilities, as well as their tribological behaviour.
Review of Si-Based Thin Films and Materials for Thermoelectric Energy Harvesting and Their Integration into Electronic Devices for Energy Management Systems
Authors: Ascencio-Hurtado Carlos Roberto , Ambrosio Lázaro Roberto C. , Estrada-López Johan Jair , Torres Jacome Alfonso
Published: May 2023

Energy harvesters are autonomous systems capable of capturing, processing, storing, and utilizing small amounts of free energy from the surrounding environment. Such energy harvesters typically involve three fundamental stages: a micro-generator or energy transducer, a voltage booster or power converter, and an energy storage component. In the case of harvesting mechanical vibrations from the environment, piezoelectric materials have been used as a transducer. For instance, PZT (lead zirconate titanate) is a widely used piezoelectric ceramic due to its high electromechanical coupling factor. However, the integration of PZT into silicon poses certain limitations, not only in the harvesting stage but also in embedding a power management electronics circuit. On the other hand, in thermoelectric (TE) energy harvesting, a recent approach involves using abundant, eco-friendly, and low-cost materials that are compatible with CMOS technology, such as silicon-based compound nanostructures for TE thin film devices. Thus, this review aims to present the current advancements in the fabrication and integration of Si-based thin-film devices for TE energy harvesting applications. Moreover, this paper also highlights some recent developments in electronic architectures that aim to enhance the overall efficiency of the complete energy harvesting system.
Correction: Choi et al. Physiological Effect of Extended Photoperiod and Green Wavelength on the Pituitary Hormone, Sex Hormone and Stress Response in Chub Mackerel, Scomber japonicus. Fishes 2023, 8, 77
Authors: Choi Young Jae , Park Seul Gi Na Ra , Jo A-Hyun , Kim Jun-Hwan
Published: May 2023

There was an error in the original publication [...]
Uptake of Fungicide Fluopyram from Soil by Scallions during Greenhouse Cultivation
Authors: Yun Myung-Sub , Choi Hoon
Published: May 2023

Unintentional pesticide contamination in rotational crops, often caused by soil contamination from pesticide use in the preceding crops, is a major concern in a positive list system. The residue and dissipation pattern of fluopyram in soil and scallions were investigated to evaluate the uptake of fluopyram from the soil by scallions. In addition, the management concentration in soil (MCsoil) was calculated based on bioconcentration factors (BCFs) and the maximum residue limit (0.2 mg/kg) in leaf-and-stem vegetables. In a field experiment, plots in two different trials, A and B, were treated with 0.06 g fluopyram/m2 and maintained for 30 days according to OECD guidelines. Scallion seedlings were cultivated for 48 days. Soil samples were taken at three different time points: DAP (Days after planting) 0, 34, and 48. Scallion samples were collected at five different time points: DAP 20, 27, 34, 41, and 48. The initial amounts of fluopyram in soil at DAP 0 were 0.94 ± 0.03 and 0.96 ± 0.04 mg/kg in trials A and B, respectively. The half-life of fluopyram in the soil was 87–231 days. Fluopyram uptake by the roots increased over time, but fluopyram residue in the scallions decreased due to the dilution effect caused by an increase in plant weight. The residues in the scallions at DAP 48 were 0.22 ± 0.01 and 0.15 ± 0.01 mg/kg in trials A and B, respectively. The BCFs of scallions for fluopyram were 0.21–0.24 (trial A) and 0.14–0.18 (trial B). The MCsoil was proposed as 0.8 mg/kg, and may be utilized as a safe management guideline for precautionary practices to cultivate safe rotational crops.
The Reasonableness and Spatial Differences of the Food Consumption Structure of Urban and Rural Residents in China, 2015–2021
Authors: Lian Yanyan , Gu Lijuan , Yang Linsheng , Wang Li , Li Hairong
Published: May 2023

Based on residents’ food consumption data from 31 provinces in China from 2015–2021, this study analyzes the deviation in food consumption from nutrition targets and the spatial distribution characteristics of urban and rural residents in China from 2015–2021, and finds that there are irrationalities in the structure of food consumption of Chinese residents as well as regional differences in consumption. The food consumption of Chinese residents deviates from the recommended values of the Chinese Food Guide Pagoda to a certain extent, with large differences between urban and rural areas and provinces. Therefore, a new concept of food security with nutrition as the target should be established to guide residents’ food consumption scientifically and rationally, and to adopt focused attention and targeted measures for regions with serious imbalances in food consumption.
Effect of Microbial Transglutaminase Treatment on the Techno-Functional Properties of Mung Bean Protein Isolate
Authors: Moon Su-Hyeon , Cho Seong-Jun
Published: May 2023

The purpose of this study was to investigate the improvement in techno-functional properties of mung bean protein isolate (MBPI) treated with microbial transglutaminase (MTG), including water- and oil-holding capacity, gelling properties, and emulsifying capacity. MBPI dispersions were incubated with MTG (5 U/g of protein substrate) at 45 °C with constant stirring for 4 h (MTM4) or 8 h (MTM8). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that MTG treatment for different durations increased the amount of high-molecular-weight proteins in MBPI, and most of the cross-linking by MTG was terminated at 8 h. Improved water-holding capacity, gelling properties, emulsifying capacity, and stability were observed after MTG treatment, and decreased protein solubility and surface hydrophobicity were observed. Furthermore, the texture of the heat-induced gels made from MTG-treated MBPI was evaluated using a texture analyzer. MTG treatment increased the hardness, gumminess, chewiness, and adhesiveness of the heat-induced gels. Field-emission scanning electron microscopy demonstrated the enhanced hardness of the gels. This research reveals that MTG-catalyzed cross-linking may adjust the techno-functional properties of MBPI, allowing it to be used as a soy protein alternative in food products, such as plant-based and processed meats.
Optimisation and Characterisation of the Protein Hydrolysate of Scallops (Argopecten purpuratus) Visceral By-Products
Authors: Chasquibol Nancy , Gonzales Billy Francisco , Alarcón Rafael , Sotelo Axel , Márquez-López José Carlos , Rodríguez-Martin Noelia M. , del Carmen Millán-Linares María , Millán Francisco , Pedroche Justo
Published: May 2023

In this research, scallops (Argopecten purpuratus) visceral meal (SVM) and defatted meal (SVMD) were analysed for their proximal composition, protein solubility, and amino acid profile. Hydrolysed proteins isolated from the scallop’s viscera (SPH) were optimised and characterised using response surface methodology with a Box-Behnken design. The effects of three independent variables were examined: temperature (30–70 °C), time (40–80 min), and enzyme concentration (0.1–0.5 AU/g protein) on the degree of hydrolysis (DH %) as a response variable. The optimised protein hydrolysates were analysed for their proximal composition, yield, DH %, protein solubility, amino acid composition, and molecular profile. This research showed that defatted and isolation protein stages are not necessaries to obtain the hydrolysate protein. The conditions of the optimization process were 57 °C, 62 min and 0.38 AU/g protein. The amino acid composition showed a balanced profile since it conforms to the Food and Agriculture Organisation/World Health Organisation recommendations for healthy nutrition. The predominant amino acids were aspartic acid + asparagine, glutamic acid + Glutamate, Glycine, and Arginine. The protein hydrolysates’ yield and DH % were higher than 90% and close to 20%, respectively, with molecular weight between 1–5 kDa. The results indicate that the protein hydrolysates of scallops (Argopecten purpuratus) visceral by product optimised and characterised was suitable a lab-scale. Further research is necessary to study the bioactivity properties with biologic activity of these hydrolysates.
Structural Characteristics and Immunomodulatory Effects of Melanoidins from Black Garlic
Authors: Song Xiwang , Xue Liangyu , Geng Xiaoyuan , Wu Jianfu , Wu Tao , Zhang Min
Published: May 2023

Melanoidins are considered to have several biological activities. In this study, black garlic melanoidins (MLDs) were collected using ethanol solution extraction; 0%, 20%, and 40% ethanol solutions were used for chromatography. Three kinds of melanoidins were produced by macroporous resin, named MLD-0, MLD-20, and MLD-40. The molecular weight was determined, and the infrared and microscopic structures were studied. In addition, Balb/c mice were induced with cyclophosphamide (CTX) to establish an immune deficiency model to evaluate the immune efficacy of black garlic melanoidins (MLDs). The results showed that MLDs restored the proliferation and phagocytosis ability of macrophages, and the proliferation activity of B lymphocytes in the MD group was 63.32% (♀) and 58.11% (♂) higher than that in the CTX group, respectively. In addition, MLDs alleviated the abnormal expression of serum factors such as IFN-γ, IL-10, and TNF-α. 16SrDNA sequencing of intestinal fecal samples of mice showed that MLDs changed the structure and quantity of intestinal flora, and especially that the relative abundance of Bacteroidaceae was significantly increased. The relative abundance of Staphylococcaceae was significantly reduced. These results showed that MLDs improved the diversity of intestinal flora in mice, and improved the adverse state of immune organs and immune cells. The experiments confirm that black garlic melanoidins have potential value in immune activity, which provides an important basis for the development and utilization of melioidosis.
Encapsulation of Bioactive Peptides by Spray-Drying and Electrospraying
Authors: Berraquero-García Carmen , Pérez-Gálvez Raúl , Espejo-Carpio F. Javier , Guadix Antonio , Guadix Emilia M. , García-Moreno Pedro J.
Published: May 2023

Bioactive peptides derived from enzymatic hydrolysis are gaining attention for the production of supplements, pharmaceutical compounds, and functional foods. However, their inclusion in oral delivery systems is constrained by their high susceptibility to degradation during human gastrointestinal digestion. Encapsulating techniques can be used to stabilize functional ingredients, helping to maintain their activity after processing, storage, and digestion, thus improving their bioaccessibility. Monoaxial spray-drying and electrospraying are common and economical techniques used for the encapsulation of nutrients and bioactive compounds in both the pharmaceutical and food industries. Although less studied, the coaxial configuration of both techniques could potentially improve the stabilization of protein-based bioactives via the formation of shell–core structures. This article reviews the application of these techniques, both monoaxial and coaxial configurations, for the encapsulation of bioactive peptides and protein hydrolysates, focusing on the factors affecting the properties of the encapsulates, such as the formulation of the feed solution, selection of carrier and solvent, as well as the processing conditions used. Furthermore, this review covers the release, retention of bioactivity, and stability of peptide-loaded encapsulates after processing and digestion.
Production and Characterization of ACE Inhibitory and Anti-Diabetic Peptides from Buffalo and Camel Milk Fermented with Lactobacillus and Yeast: A Comparative Analysis with In Vitro, In Silico, and Molecular Interaction Study
Authors: Khakhariya Ruchita , Basaiawmoit Bethsheba , Sakure Amar , Maurya Ruchika , Bishnoi Mahendra , Kondepudi Kanthi , Padhi Srichandan , Rai Amit , Liu Zhenbin , Hati Subrota
Published: May 2023

The investigation aimed at assessing a comparative study on the production and characterization of ACE inhibitory, anti-diabetic, and anti-inflammatory activities, along with the production of ACE inhibitory and anti-diabetic peptides through the fermentation of buffalo and camel milk by Limosilactobacillus fermentum (KGL4) and Saccharomyces cerevisiae (WBS2A). The angiotensin-converting enzyme (ACE) inhibitory and anti-diabetic properties were evaluated at particular time intervals (12, 24, 36, and 48 h) at 37 °C, and we discovered maximum activity at 37 °C after 48 h of incubation. The maximum ACE inhibitory, lipase inhibitory activities, alpha-glucosidase inhibitory, and alpha-amylase inhibitory activities were found in the fermented camel milk (77.96 ± 2.61, 73.85 ± 1.19, 85.37 ± 2.15, and 70.86 ± 1.02), as compared to the fermented buffalo milk (FBM) (75.25 ± 1.72, 61.79 ± 2.14, 80.09 ± 0.51, and 67.29 ± 1.75). Proteolytic activity was measured with different inoculation rates (1.5%, 2.0%, and 2.5%) and incubation times (12, 24, 36, and 48 h) to optimize the growth conditions. Maximum proteolysis was found at a 2.5% inoculation rate and at a 48 h incubation period in both fermented buffalo (9.14 ± 0.06) and camel milk (9.10 ± 0.17). SDS-PAGE and 2D gel electrophoresis were conducted for protein purification. The camel and buffalo milk that had not been fermented revealed protein bands ranging from 10 to 100 kDa and 10 to 75 kDa, respectively, whereas all the fermented samples showed bands ranging from 10 to 75 kDa. There were no visible protein bands in the permeates on SDS-PAGE. When fermented buffalo and camel milk were electrophoresed in 2D gel, 15 and 20 protein spots were detected, respectively. The protein spots in the 2D gel electrophoresis ranged in size from 20 to 75 kDa. To distinguish between different peptide fractions, water-soluble extract (WSE) fractions of ultrafiltration (3 and 10 kDa retentate and permeate) of fermented camel and buffalo milk were employed in RP-HPLC (reversed-phase high-performance liquid chromatography). The impact of fermented buffalo and camel milk on inflammation induced by LPS (lipopolysaccharide) was also investigated in the RAW 264.7 cell line. Novel peptide sequences with ACE inhibitory and anti-diabetic properties were also analyzed on the anti-hypertensive database (AHTDB) and bioactive peptide (BIOPEP) database. We found the sequences SCQAQPTTMTR, EMPFPK, TTMPLW, HPHPHLSFMAIPPK, FFNDKIAK, ALPMHIR, IPAVFK, LDQWLCEK, and AVPYPQR from the fermented buffalo milk and the sequences TDVMPQWW, EKTFLLYSCPHR, SSHPYLEQLY, IDSGLYLGSNYITAIR, and FDEFLSQSCAPGSDPR from the fermented camel milk.
Further Development of Gasoline from the bioliq® Process with Focus on Particulate and Hydrocarbon Emissions
Authors: Michler Tobias , Niethammer Benjamin , Fuchs Constantin , Toedter Olaf , Arnold Ulrich , Koch Thomas , Sauer Jörg
Published: May 2023

The production of CO2-neutral fuels is a key technology to achieve the European Union’s targets of greenhouse gas reduction in the transport sector. For a straightforward application such as drop-in fuel, regenerative gasoline must meet emission requirements without causing significant changes in engine parameters. The objective of this work was to demonstrate the emission reduction potential of fuel from the bioliq® plant by reducing the content of heavy aromatics in the product refinement. For three blends with varying contents of bioliq® fuel, the spray behavior was studied in a pressurized chamber and the particulate and hydrocarbon emissions were investigated using a single-cylinder research engine. With increasing bioliq® fuel content, atomization was degraded by lower flash boiling at low pressure. This effect vanished at higher chamber pressures. Measurements of particulate and hydrocarbon emissions showed significant improvements of 50% to 100% and 10%, respectively, compared to previously investigated bioliq® fuel fractions from 2017. The formation of particulate emissions is virtually unaffected by the blending of bioliq® fuel, due to the absence of heavy aromatics in the refined bioliq® product. Hydrocarbon emissions increased by 20% with higher bioliq® fuel content and late injection timings due to inferior mixture formation as a result of slightly reduced atomization. However, near the optimum injection timing, the hydrocarbon emissions are independent of the bioliq® fuel admixture.
Current State of Antimicrobial Treatment of Lower Respiratory Tract Infections Due to Carbapenem-Resistant Acinetobacter baumannii
Authors: Merli Marco , D’Amico Federico , Travi Giovanna , Puoti Massimo
Published: May 2023

Carbapenem-resistant Acinetobacter baumannii (CRAB) is a worldwide non-fermenting Gram-negative bacillus responsible for potentially severe nosocomial infections, especially in critically ill patients. CRAB tends to colonize inert surfaces and epithelia, especially the respiratory tract of mechanically ventilated patients, and may then become responsible for lower respiratory tract infections, probably the more challenging infection due to the site and the multidrug-resistant phenotype which makes it difficult to establish an effective antimicrobial regimen. Despite its diffusion, data regarding the treatment of CRAB are mainly retrospective and usually heterogeneous. Current international consensus guidelines prefer the use of ampicillin/sulbactam, but the strength of recommendation and grade of evidence tend to be weak to moderate. Moreover, no specific recommendation is given for different sites of infections. The recently introduced cefiderocol still received a recommendation against its use due to the results of the first randomized clinical trial, though retrospective and observational experiences showed favourable outcomes in this setting. We reviewed the major antibacterial drugs active against CRAB and discussed their combination in lower respiratory tract infections.
Internal Structure and Reactivations of a Mass Movement: The Case Study of the Jacotines Landslide (Champagne Vineyards, France)
Authors: Bollot Nicolas , Pierre Guillaume , Grandjean Gilles , Fronteau Gilles , Devos Alain , Lejeune Olivier
Published: May 2023

The Jacotines landslide is representative of the large mass movements that affect the Champagne vineyards. Understanding the subsurface structure of these slopes and the mechanisms leading to sliding events is of a great interest, particularly for winegrowers who produce Champagne. This knowledge is generally used to elaborate accurate hazard assessment maps, which is an important feature in land use planning. The approach presented is based on the integration of geophysical imaging (seismic wave velocity and electrical resistivity), lithostratigraphic analysis (drilling core) and geomorphological investigations (surface landforms) to reconstruct the relations between the landslide structure, surface water flow, groundwater regime and the overall slope stability. A first phase of instability resulting in a large rotational slip probably occurred during the Late Glacial Period in morphoclimatic conditions characterized by an excess of water. A second one, still active, leading to superficial reactivations and relates to present hydrogeological conditions determined by the internal structure of the landslide.
The Assessment of Natural Vaginal Delivery in Relation to Pregnancy-Related Anxiety—A Single-Center Pilot Study
Authors: Michalik Anna , Pracowity Michalina , Wójcicka Lucyna
Published: May 2023

Background: Pregnancy-related anxiety (PrA) is a specific type of anxiety experienced during the perinatal period. It may concern a person’s health and physical appearance, fetal development, hospital and health care experiences, impending childbirth, and early parenthood. PrA is considered to be a stronger predictor of adverse pregnancy outcomes than general anxiety and depression. The purpose of this research was to conduct a pilot study and evaluate the course of vaginal birth (VB) in relation to PrA levels in a population of pregnant women with low obstetrical risk. Methods: This cross-sectional exploratory study included 84 pregnant women (with a mean age of 28.61 ± 4.99) (without cesarean section (CS) indications and with a low risk of complications during VB). Research questionnaires were distributed and filled in in person during the course of hospitalization. Groups that varied at the level of PrA were compared using the Wilcoxon rank-sum test, Fisher’s exact test, or chi-square test of independence, as appropriate. Results: More than two-thirds of the respondents (72.6%) were medicated in labor. Women with high PrA, selected based on a cut-off point with a total PRAQ-R2 score of 60, experienced significantly longer first (start of established labor to fully dilated cervix) and second (lasts from when cervix is fully dilated until the birth) periods of labor, instrumental delivery, or emergency CS. In the group with high PrA levels, a episiotMmentation of evidence-based recommendations for the affected population to identify and further treat women with elevated levels of PrA.
Factors Associated with the Underestimation of Manual CPAP Titration Pressure
Authors: Chen Po-Yueh , Viet-Nhi Nguyen-Kieu , Chen Yen-Chun , Kao Yi-Lin , Dang Luong Huu , Hung Shih-Han
Published: May 2023

During the SARS-CoV-2 pandemic, the use of in-laboratory positive airway pressure (PAP) titration studies was not routinely suggested. PAP pressure prediction calculations are emerging as alternative methods for the treatment of these patients. The underestimation of PAP titration pressure usually leads to unsatisfactory results for PAP therapy. This study aimed to evaluate the factors associated with the underestimation of PAP titration pressure when using PAP pressure prediction equations. Estimated PAP pressure formulas based on body mass index (BMI) and apnea-hypopnea index (AHI) were chosen to validate the accuracy of equations in the successful prediction of titration pressure. Among 341 adult patients diagnosed with obstructive sleep apnea (OSA) by overnight polysomnography (PSG) and who received overnight PAP titration in order to select a successful pressure, the mean age of the total subjects was 55.4 years old and 78.9% of patients were male. The average BMI and AHI scores were 27.1 ± 4.8 and 37 ± 21.7, respectively. After multivariate stepwise regression analysis, the odds ratio of participants with a pretitration AHI was 1.017 (95% CI: 1.005–1.030). Only the severity of OSA was significantly different between the underestimated group and the adequately assessed group. In conclusion, a high AHI, but not BMI, is associated with an underestimated CPAP titration pressure in adult patients with OSA.
Unpacking the Lay Epidemiology of Cervical Cancer: A Focus Group Study on the Perceptions of Cervical Cancer and Its Prevention among Women Late for Screening in Norway
Authors: Aasbø Gunvor , Hansen Bo , Waller Jo , Nygård Mari , Solbrække Kari
Published: May 2023

Nonattendance for cervical cancer screening is often understood in terms of a lack of ‘appropriate’ or ‘correct’ knowledge about the risks and prevention of the disease. Few studies have explored how lay persons—the users themselves—interpret and contextualise scientific knowledge about cervical cancer. In this study, we address the following research question: How is the epidemiology of cervical cancer and its prevention discussed among women who are late for cervical cancer screening in Norway? We completed nine focus group interviews (FGIs) with 41 women who had postponed cervical screening. The analyses were both inductive and explorative, aiming to unpack the complexity of lay understandings of cervical cancer. Interactive associations expressed in the FGIs reflected multiple understandings of aetiology and risk factors, screening, and interpretations of responsibility for acquiring cervical cancer. The term ‘candidacy’ was employed to provide an enhanced understanding of lay reasoning about the explanations and predictions of cervical cancer, as reflected in the FGIs. Both interpretations of biomedical concepts and cultural values were used to negotiate acceptable and nuanced interpretations of candidacy for cervical cancer. Uncertainties about risk factors for acquiring cervical cancer was an important aspect of such negotiations. The study’s findings provide an in-depth understanding of the contexts in which screening may be rendered less relevant or significant for maintaining health. Lay epidemiology should not be considered inappropriate knowledge but rather as a productive component when understanding health behaviours, such as screening attendance.
Evaluation of Clinical Symptoms Improvement by Cognitive Behavioral Therapy Using a Smartphone Application in Patients with Temporomandibular Disorder
Authors: Hwangbo Na-Kyung , Woo Keon-Cheol , Kim Seong-Taek
Published: May 2023

Since the start of the 2019 coronavirus pandemic, interest in digital therapeutics (DTx) has increased. Temporomandibular disorder (TMD) fundamentally requires cognitive behavioral therapy (CBT), including physical self-regulation. An application that records TMD pain and parafunctional activities for CBT has recently been developed. However, evidence of the reduction of clinical symptoms in patients via repetitive software-driven CBT is lacking. The purpose of the present study was to evaluate the impact of applications that support CBT regarding the performance of CBT and the improvement of clinical symptoms in temporomandibular joint patients. From 20 October 2020 to 7 January 2021, we randomly assigned 41 participants diagnosed with TMD to control (conventional treatment) and experimental (conventional treatment + application use) groups. We randomly assigned 41 participants diagnosed with TMD to control (conventional treatment) and experimental (conventional treatment + application use) groups. Improvements regarding the number of tender points, mouth opening, visual analog scale score, pain level upon palpation, joint sound, and stress were compared between the two groups. Compared with the control group, the experimental group showed significant improvements in the number of tender points and degree of mouth opening. They also showed improvements in pain level, joint sound, and locking, although not statistically significantly, as compared with the control group. Thus, further studies with a greater sample size need to be conducted to confirm the findings. Nevertheless, our results showed that repetitive cognitive behavioral therapy using a smartphone application can be used as digital therapeutics for temporomandibular disorder patients.
Overexpression of RrGGP2 and RrDHAR Increases Ascorbic Acid Content in Tomato
Authors: Liu Zeyang , Rao Tianzhi , Ludlow Richard A. , Yan Yali , Lu Min , An Huaming
Published: May 2023

Ascorbic acid (AsA) is the most abundant antioxidant in plants and is an important nutritional index for agricultural products. Some plants, such as Rosa roxburghii Tratt., contain exceptionally high levels of AsA, but are relatively unpalatable. In view of its role in human health, as well as plant growth and development, we examined the effects of two important AsA regulatory genes from R. roxburghii in tomato, with the aim of producing a crop of higher nutritional quality. RrGGP2 and RrDHAR were cloned from R. roxburghii fruit. The overexpression vectors were made using 35S promoters and mediated by Agrobacterium tumefaciens to obtain the overexpression lines. A PCR and qRT-PCR verified that the two genes had been inserted and overexpressed in the tomato leaves and fruits. The results showed that the overexpression of RrGGP2 increased tomato leaf and fruit AsA content by 108.5% and 294.3%, respectively, while the overexpression of RrDHAR increased tomato leaf and fruit AsA content by 183.9% and 179.9%. The overexpression of RrGGP2 and RrDHAR further changed the expression of genes related to AsA metabolism, and the upregulation of one such gene, SlGGP, may have contributed greatly to the increase in AsA. Results here indicate that RrGGP2 contributes more towards fruit AsA accumulation in tomato than RrDHAR.
Publicly Underrepresented Genocides of the 20th and 21st Century: A Review
Authors: Diboyan Larra M. , Goliath Jesse R.
Published: May 2023

Forensic anthropologists have been involved in investigating genocide and crimes against humanity for many decades. Raphael Lempkin first coined the term “genocide” in 1944, and in 1946, the United Nations General Assembly codified it as an independent crime. However, there has not been a systematic review available to better understand the history of many of these atrocities. Moreover, many of these events have not been discussed outside the cultures and individuals affected. This targeted literature review will discuss work on historic, lesser-known, modern genocides, and finally, the humanitarian forensic work being conducted in the field and digitally. Such events discussed include Herero and Namaqua, Sayfo, Armenian, Holodomor, Nanking (Nanjing), Romani, Palestinian, Bangladesh, Ethiopia, Sikh, and Rohingya genocides. Work being done in this important sector of research is a critical development for not only recognizing these crimes but also for documenting and protecting the evidence of these human rights violations.
Indoor Volatile Organic Compounds: Concentration Characteristics and Health Risk Analysis on a University Campus
Authors: Jin Shengjia , Zhong Lu , Zhang Xueyi , Li Xinhe , Li Bowei , Fang Xuekun
Published: May 2023

Volatile organic compounds (VOCs) are major indoor air pollutants that contain several toxic substances. However, there are few studies on health risk assessments of indoor VOCs in China. This study aimed to determine the concentration characteristics of VOCs on college campuses by collecting VOC samples from different locations on campus during different seasons combined with the exposure times of college students in each location obtained from a questionnaire survey to assess the possible health risks. The highest total VOC concentration (254 ± 101 µg/m3) was in the dormitory. The seasonal variation of TVOC concentrations was related to the variation of emission sources in addition to temperature. Health risk assessments of VOCs were evaluated using non-carcinogenic and carcinogenic risk values, represented by hazard quotient (HQ) and lifetime cancer risk (LCR), respectively. The non-carcinogenic risks at all sampling sites were within the safe range (HQ < 1). Dormitories had the highest carcinogenic risk, whereas the carcinogenic risk in the other three places was low (with LCR < 1.0 × 10−6). Moreover, 1,2-dichloroethane was identified as a possible carcinogenic risk substance in the dormitory due to its high LCR (1.95 × 10−6). This study provides basic data on health risks in different locations on campus and a basis for formulating measures to improve people’s living environments.
Crime Risk Analysis of Tangible Cultural Heritage in China from a Spatial Perspective
Authors: Ding Ning , Zhai Yiming , Lv Hongyu
Published: May 2023

Tangible cultural heritage is vulnerable to various risks, particularly those stemming from criminal activity. Through analyzing the distribution and flow of crime risks from a spatial perspective based on quantitative methods, risks can be better managed to contribute to the protection of cultural heritage. This paper explores and summarizes the spatial characteristics of crime risks from 2011 to 2019 in China. Firstly, the average nearest neighbor (ANN) and the Jenks Natural Breaks Classification method showed that the national key protected heritage sites (NPS) and crime risks exhibit clustering features in space, and most of the NPS were located in the middle and lower reaches of the Yangtze River and the Yellow River. Secondly, the economy has no impact on crime risks in the spatial statistical analysis. However, the population density, distribution of NPS, and tourism development influenced specific types of crime risks. Finally, Global Moran’s I was used to examine the strong sensitivity between crime risks and cultural relics protection policies. The quantitative results of this study can be applied to improve strategies for crime risk prevention and the effectiveness of heritage security policy formulation.