bluuebunny/crossref_metadata_2025 huggingface dataset:
Screening whole proteome of Aedes aegypti and identification of potential targets for in-silico molecular and structural interaction studies against natural bioactives v1
Authors: S Setlur Anagha , K Chandrashekar , Sarkar Manas , Niranjan Vidya
Published: May 2022

As a dangerous etiological agent for dengue, chikungunya, zika and yellow fever, it is essential to combat the incidences of Aedes aegypti, by using repellents. However, chronic overuse of synthetic repellents has led to possibilities of adverse side effects in humans. As a consequence, scientists and researchers are now shifting the focus of research on developing natural alternatives to these repellents. In such a case, the present study aimed to devise a standard protocol that can screen the whole proteome of A. aegypti and identify the major proteins that can be targeted by natural bioactives to produce repellents. To study the binding of the natural actives and the targets, a whole proteome analysis was carried out by finding the reference proteome of the organism, performing a literature survey to identify the potential targets, understanding the circadian rhythm of A. aegypti to identify the proteins expressed in the dark and light cycles, and shortlisting the targets by analyzing the common conserved domains of query sequences. Twenty protein target categories were identified, out of which 309 protein sequences were modelled using standalone tool- RaptorX. These structures were validated using Ramachandran plots from SAVES v6.0. Molecular docking studies using POAP, between the selected representative of the twenty protein targets and the natural bioactives revealed negative binding energies. Those that had the least negative energies were taken forward for 100ns molecular dynamic simulation studies, from which the docked complex stabilities were noted and the conformational changes induced during simulations were revealed. This protocol allows whole proteome analysis that will enable identification of major protein targets that the naturals can act upon, and further reveals the effectiveness of the use of naturals against these proteins, thereby, implying the use of this methodology for whole proteome analysis of other organisms as well. Keywords: Whole proteome, Aedes aegypti, conserved domains, homology modelling, docking, molecular dynamic simulations
MWP5 Final Project: 5 findability opportunities to access the portfolio dataset v1
Authors: Iarkaeva Anastasiia
Published: May 2022

This protocol was created as part of the Information Science Master's program project MWP5 Digital Information Management at the Humboldt University of Berlin. It aims to help a potential user group of the decision-making stakeholders to find the examined dataset for cluster modeling (https://doi.org/10.6084/m9.figshare.12743639.v1). Overall five opportunities are both described textually and shown visually. In all cases, except the DOI system, the search results can be reduced by applying multiple filters to find the wanted dataset faster and more precisely.
Native gel lipid binding assay v1
Authors: not provided patreece.suen
Published: May 2022

This protocol details how to perform a native gel lipid binding assay to evaluate the number of lipids bound per molecule of 3xFLAG-SHIP164Δ901-1099
Testimony on a successful lab protocol to disrupt Chlorella vulgaris microalga cell wall v1
Authors: Coelho Diogo
Published: May 2022

Over the last decades, microalgae have gained popularity due to demand for novel environmental green solutions and development of innovative mass-production sources for multiple processes, including animal feed and human diet, turningmicroalgae into an exquisite candidate for several ecofriendly technologies. Notwithstanding, there is a catch. Most species of microalgae, as the case of common Chlorella vulgaris (C. vulgaris)display a recalcitrant cell wall, characterized by a complex matrix of polysaccharides and glycoproteins, which constitutes a major barrier for monogastric species digestibility and extraction of inner valuable nutritional compounds. To overcome this limitation, the development of feed enzymes, in particular Carbohydrate-Active enZymes (CAZymes) with capacity to disrupt C. vulgariscell wallmay contribute to improve the bioavailability of these microalgae compounds in monogastric diets, namely at high levels of incorporation. In order to disclosure novel combination of feed enzymes to disrupt C. vulgaris cell wall, a lab protocol was implemented by our research team containingthe following key steps: after microalgae cultivation and having available a repertoire of two hundred pre-selected CAZymes produced by high-throughput technology, thestep 1 is the individual screening of the most functional enzymes on disrupting C. vulgariscell wall (versus a control, defined as the microalgae suspension incubated with PBS) and the determination of reducing sugars released by the 3,5-dinitrosalicylic acid (DNSA) method; step 2 concerns on finding the best CAZymes cocktail, testing the synergistic effect of enzymes, to disrupt C. vulgariscell wall (in parallel with running the control) along with characterization of each enzyme thermostability and resistance to proteolytic attack, to which feed enzymes are subjected in the animal gastrointestinal tract; step 3 is the assessment of C. vulgaris cell wall degradation degree by measuring the amount of reducing sugars released by the DNSA method, fatty acid analysis by gas chromatography (GC) with flame ionization detector(FID), oligosaccharides quantification byhigh performance liquid chromatography(HPLC) equipped with an electrochemical detector (ECD),protein content by the Kjeldahl method, and various pigments (chlorophylls a and b, and total carotenoids) in the supernatant. In the correspondent residue, we also assessed cellular counting using a Neubauer chamber by direct observation on a bright-field microscope and fluorescence intensity, after staining with Calcofluor White for both control and CAZymes cocktail treatments, on a fluorescence microscope. Beyond animal feed industry with impact on human nutrition, our lab protocol may increase the yield in obtaining valued constituents from C. vulgarismicroalga for other biotechnological industries.
Human Knee Cartilage Collection Protocol for Single Cell RNAseq v1
Authors: not provided molmer , Lotz Martin
Published: May 2022

Cartilage from the medial femoral condyle is shaved for Single Cell RNAseq processing. The attached image indicates where the cartilage shavings are collected from.
Cryo-EM sample preparation v1
Authors: not provided patreece.suen
Published: May 2022

This protocol details how to prepare cryo-EM samples of 3xFLAG-SHIP164Δ901-1099
TMA-TNP Section Map and Slide Processing - Phase 3 v1
Authors: S Feiler Heidi
Published: May 2022

Human Tumor Atlas Tissue MicroArrary TNP (TMA-TNP) The Tissue MicroArray (TMA) TNP will extend the SARDANA-TNP characterization and analytics methodologies for evaluation and validation on a large array of breast tumor samples that provide a broad spectrum of disease state and subtype. A commercially available anonymized breast tumor TMA was purchased and sections distributed to participating HTAN Centers. Some deidentified basic clinical data will also be provided. The participating HTAN Centers will characterize the FFPE specimens (e.g. by imaging) and generate a spatially resolved cell type/state census using each Center’s method of choice. Data will then be recorded in a common repository to enable joint analysis. This TNP has two specific Aims focused on (i) Data Collection and (ii) Data Coordination and Analysis. Individual Centers can participate in one or both aims. The custom TMA design for this project was generated by Dr. Koei Chin (OHSU) and sent to Pantomics for custom TMA FFPE block generation and sectioning. The TMA design and clinical sample descriptions can be found here: dx.doi.org/10.17504/protocols.io.bn2fmgbn . This protocol describes the procedure by which the OHSU OMS Atlas HTAN Center sectioned and distributed TMA sections for Phase 3 analysis.
Effectiveness of exercise therapy and self-management education to improve physical activity levels in patients with acute exacerbations of chronic obstructive pulmonary disease: protocol of a systematic review and meta-analysis v2
Authors: Yamamoto Koki , Takayama Mami , Nonoyama Tadayoshi , Kon Yusuke , Saimon Yoshiki , Kitagawa Takashi
Published: May 2022

We aimed to evaluate the effectiveness of exercise therapy and self-management education in improving physical activity levels (PA) in patients with acute exacerbations of chronic obstructive pulmonary disease (AECOPD) and to provide future research directions. We plan to include all randomized controlled trials (RCTs) of exercise therapy and self-management education or either intervention alone designed to improve PA in patients with AECOPD. We will determine the differential effects of exercise therapy and self-management education interventions on daily steps, moderate to vigorous activity time (MVPA), and sedentary equivalent time (SB) (P: AECOPD, I: 1. exercise therapy, 2. self-management education, 3. exercise therapy and self-management education, C: usual care, O: steps per day, MVPA, SB). In this review, we plan to use five databases. Two reviewers will screen articles to identify studies for inclusion.For risk of bias assessment, we will use Risk of Bias 2 tool.
NCBI submission protocol for SARS-CoV-2 wastewater data: SRA, BioSample, and BioProject v6
Authors: Timme Ruth , Balkey Maria
Published: May 2022

PURPOSE: This method was developed at the FDA’s Center for Food Safety and Applied Nutrition for GenomeTrakr’s pandemic response project, monitoring SARS-CoV-2 variants in wastewater; however, this protocol was written to be broadly applicable for all wastewater sequence data submissions to NCBI. Protocols developed for this project cover wastewater collection, concentration, RNA extraction, RT-qPCR, library prep, genome sequencing, quality control checks, and data submission to NCBI. This protocol covers the last step of making your data public at NCBI. Specifically, it provides the steps to establish a new NCBI submission environment for your laboratory, including the creation of new BioProject(s) and submission groups. Once these are step up, the protocol then walks through the process for submitting raw reads to SRA and sample metadata to BioSample through the Submission portal. For new submitters, there's quite a bit of groundwork that needs to be established before a laboratory can start its first data submission. We recommend that one person in the laboratory take a few days to get everything set up in advance of when you expect to do your first data submission. If you need a pipeline for frequent or large volume submissions, follow Step 1 in this protocol to get your NCBI submission environment established, then contact gb-admin@ncbi.nlm.nih.gov to set up an account for submitting through the API. Version updates: V2: minor edits to the BioSample and SRA templates V3: Adapted the protocol to be more broadly applicable to submitters outside of FDA's wastewater project. Updates were also made to both metadata templates, including a new attribute to the SRA metadata template, called "enrichment_kit". V4: updates to BioSample and SRA templates: expanded picklists, addition of specimen processing attributes for including replicate info, and the removal of target_extract attribute for reporting level of target found in the sample. V5: includes guidance for submitting BioSamples with no linked sequence data. V6: Updated templates. BioSample: added picklist for PCR concentration units. SRA: added new quality control attributes.
Modified NEBNext® VarSkip Short SARS-CoV-2 Library Prep Kit for Illumina Platforms - adapted for wastewater samples v3
Authors: Ramachandran Padmini , Walsky Tamara , Windsor Amanda , Hoffmann Maria , Grim Chris
Published: May 2022

PURPOSE: This method was developed at the FDA’s Center for Food Safety and Applied Nutrition for GenomeTrakr’s pandemic response project, monitoring SARS-CoV-2 variants in wastewater. Protocols developed for this project cover wastewater collection, concentration, RNA extraction, RT-qPCR, library prep, genome sequencing, quality control checks, and data submission to NCBI. This modified protocol details methods for cDNA synthesis and library preparation for sequencing of wastewater samples containing SARS-CoV-2. The protocol is based primarily on the NEBNext® ARTIC SARS-CoV-2 Library Prep Kit (Illumina®), NEB #E7650S/L 24/96 reactions, with a few modifications. Primarily, VarSkip Short primers are used in place of the ARTIC V3 primers. These primers are available in the NEBNext®ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®); however, for optimal variant detection from wastewater, sequenced fragments should be as large as possible, so we discourage fragmentation prior to end prep. There are a couple of decision points in this protocol. Examining cDNA amplicon samples on an Agilent TapeStation system or similar fragment analyzer is extremely helpful in making these decisions. Version Updates: V3- Controlled vocabulary for positiveControl and negativeControl has been added to the Sample Sheet.
Stroke volume and cardiac output during 6 minute-walk tests are strong predictors of maximal oxygen uptake in people with stroke v1
Authors: not provided Fangliu
Published: May 2022

The 6-minute walk test (6MWT) is a field test commonly used to predict VO2peakin people after stroke. Inclusion of cardiodynamic variables measured by impedance cardiography (ICG) during a 6MWT has been shown to improve prediction of peak oxygen consumption (VO2peak) in healthy adults but these data have not been considered in people after stroke. This study investigates whether the prediction of VO2peak can be improved by the inclusion of cardiovascular indices derived by impedance cardiography (ICG) during the 6MWT in people with strokeThis study investigates whether the prediction of VO2peak can be improved by the inclusion of cardiovascular indices derived by impedance cardiography (ICG) during the 6MWT in people with stroke This study investigates whether the prediction of VO2peak can be improved by the inclusion of cardiovascular indices derived by impedance cardiography (ICG) during the 6MWT in people with stroke. Our study showed that inclusion of SV and CO measured during the 6MWT in stroke patients further improved the VO2peak prediction power compared to using 6MWD as a lone predictor.
Illumina MiSeq Dual-barcoded Two-step PCR Amplicon Sequencing Protocol v1
Authors: M. U'Ren Jana , A. Elizabeth Arnold not provided
Published: May 2022

Background Information High throughput amplicon sequencing is an extremely sensitive technique. The lab environment is full of airborne bacterial and fungal cells as well as bacterial and fungal genomic DNA from cultures, and so extreme care must be taken to keep reactions uncontaminated and in preventing cross-contamination. This protocol was modified from the IBEST Geomics Core at University of Idaho. It utilizes a 2-step PCR, where the first PCR amplifies the specific region of interest (e.g., ITS or 16S V4), and the second PCR adds adapters and barcodes to these amplicons. The target specific primers include (in addition to the standard primer sequence) the universal sequences CS1 and CS2, which provide the bases used in the second PCR to extend the amplicons with Illumina adapters and sample-identification barcodes. Illumina adapters and sample-identification barcodes are not included in the region-specific primers used in PCR1. This allows for maximum flexibility in choosing which primers to use and provides the ability to swap out targets, or include multiple targets in the same sequencing reaction, without needing to purchase a large number of barcoded target specific primers. Barcodes are included in both adapters, therefor a pair of barcodes is used to uniquely identify each sample during bioinformatic processing. This allows us to use 32 barcode pairs to uniquely identify 1024 samples (32 x 32). Phase-shifted primers are used to introduce 1, 2, and/or 3 additional bases to allow for the maximum amount of “sequence diversity” in the first 4 bases. This is important because of how the Illumina sequencers detect clusters on the flowcell during sequencing. If sequences are too similar (as is often the case with amplicons), then sequencing yields will be much lower than expected. 2-bp linkers and phase-shifting bases were chosen to have low identity between primers and the target sequence. Tagged primers were ordered from IDT (standard desalting; not HPLC-purified), re-suspended at 100 μM using a new bottle of molecular grade water in the PCR-free hood, and mixed in equimolar concentration (250 μl each). Working stocks were diluted to 50 μM for PCR1.
Purify 247 Keto Gummies v1
Authors: Shi H
Published: May 2022

Health care reform adds various taxes on health care that insurance companies will have to collect and pay, but they're just going to pass it right through to us, the consumer.
ASt Test Method v1
Authors: not provided AStracke.Privat
Published: May 2022

Sample preparation
Next Generation Sequencing of HIV-1 Drug Resistant Mutations v1
Authors: M McGruder Rawson Brenna
Published: May 2022

The Florida Department of Health's Bureau of Public Health Laboratories in Jacksonville has developed a protocol for the Next Generation Sequencing (NGS) of HIV, primarily for the purpose of drug-resistant mutation identification. This HIV-1 protocol uses amplicon-based sequencing based on primers designed by the BEEHIVE Consortium (https://www.beehive.ox.ac.uk/). The amplified pol gene regions can be used in both genotyping and drug resistance determination. Our protocol utilizes newer enzymes with higher fidelity for sequencing and Illumina sequencing technology. We have cross verified 3 different Illumina Sequencing platforms to ensure that all produce equivalent results so that in the event of a surge samples can be sequenced quickly and in mixed-species pools. The NGS data generated can also be used in surveillance and outbreak monitoring, giving epidemiologist more information about circulating viral genomes. There is also the potential that this protocol can be expanded to whole genome sequencing for HIV-1. The imminent sunsetting of ViroSeq (Abbott Molecular) has required many labs to look for new methods to continue identifying HIV-1 drug resistance strains for both clinical management and epidemiological study. NGS was chosen as it is more cost effective than investing in a single pathogen platform. NGS allows for one sample to produce results and data that can aid not just a patient but an entire population.
Scaffold Mapping Protocol - Version 1.1.0 v2
Authors: Lin Mabelle , Sharma Sonia
Published: May 2022

The scope of this protocol covers the steps involved in the mapping of image data to organ scaffolds.
Stranded Transcript Count Table Generation from Long Reads v14
Authors: A Eccles David
Published: May 2022

This protocol is for comparing different samples at the transcript level, using long reads that are mapped to transcripts. Input(s): demultiplexed and oriented fastq files (see protocol Preparing Reads for Stranded Mapping), transcript reference fasta file, annotation file Output(s): transcript table, sorted by differential coverage, annotated with gene name / description / location
U-uF-BM# Bifurcating Mixing Microfluidic Chips v1
Authors: S Serhat
Published: May 2022

This is the user manual for U-uF-BM# Bifurcating Mixing Microfluidic Chips of uFluidic.com. It is designed to make nanoencapsulation liposomes and polimeric nanoparticles.
Spartan Testo Boost Pro Worldwide Most Selling Product! Now Available In USA? v1
Authors: not provided senhuestein
Published: May 2022

Spartan Testo Boost Pro Worldwide Most Selling Product! Now Available In USA?
Counting kidneys v1
Authors: J Barrero Gabriel , Palmer Abraham , Polesskaya Oksana
Published: May 2022

Dissecting and counting kidneys in rats.
Availability of Open Citations from Open Journals in Crossref v1
Authors: Brembilla Davide , Catizone Chiara , Venditti Giulia
Published: May 2022

This protocol is for the research about the availability of Open Citations from Open Journals in Crossref. The goal is to find out how many papers from DOAJ journals are available on Crossref, whether their metadata is available and the origin of their DOI. Our research involves the articles from Open Access journals in DOAJ and their data on Crossref. We scouted the availability of these articles’ reference lists, the presence of IDs such as DOIs and the entities responsible for their specification. This analysis was carried out by querying the DOAJ and Crossref’s APIs, creating datasets combining the two. The data will be further analysed to verify the distribution of the provenance of the articles' DOI.
Entrenamiento en habilidades no técnicas versus entrenamiento estándar en estudiantes de internado para disminuir error médico en crisis en simulación: Ensayo Clínico Controlado Aleatorizado. Estudio TECRISIS v1
Authors: Zamudio Mario
Published: May 2022

Protocolo el estudio Tecrisis Ensayo clínico controlado
Human Pregnant Fallopian Tube Tissue Collection and Preservation Methods - UCSD Female Reproductive TMC v1
Authors: Stanley Valentina , Lindsay-Hewett Scott , Parast Mana , Laurent Louise
Published: May 2022

Human pregnant fallopian tube tissue collection and storage protocol for HuBMAP's UCSD Female Reproductive TMC.
Coverage of open citations in DOAJ journals v2
Authors: Dami Constance , Bertozzi Alessandro , Manca Chiara , Kucuk Umut
Published: May 2022

This is the protocol for the research of the coverage of open citations in DOAJ journals. Our goal is to find out: about the coverage of articles from open access journals in DOAJ journals as citing and cited articles, how many citations do DOAJ journals receive and do, and how many of these citations involve open access articles as both citing and cited entities, as well as the presence of trends over time of the availability of citations involving articles published in open access journals in DOAJ journals.
LRRK2RCKW Widefield fluorescence microtubule binding assay v1
Authors: M. Snead David
Published: May 2022

This assay uses TMR labeled LRRK2 or LRRK1 RCKW to measure binding to microtubules in vitro. Created by David Snead. Adapted to protocols.io by Mariusz Matyszewski. As used in Snead, Matyszewski, Dickey et al. 2022.
Apple Cider Vinegar Gummies v1
Authors: Keto Gummies Apple
Published: May 2022

Apple Cider Vinegar Gummies
Human Pregnant Uterine Myometrium Tissue Collection and Preservation Methods - UCSD Female Reproductive TMC v1
Authors: Stanley Valentina , Lindsay-Hewett Scott , Parast Mana , Laurent Louise
Published: May 2022

Human pregnant uterine myometrium tissue collection and storage protocol for HuBMAP's UCSD Female Reproductive TMC.
Shipping wastewater samples to FDA-CFSAN v3
Authors: Balkey Maria , not provided Tina.Pfefer
Published: May 2022

This SOP provides guidance for shipping wastewater samples to the US FDA Center for Food Safety and Applied Nutrition (CFSAN), covering specific steps for registering samples with CFSAN and for the preparation of shipments (documentation, packaging, notifications). We recommend that samples are shipped in two batches, one batch after 3 months of sampling and one batch at the conclusion of your sampling (~6 months).
DNA extraction and Nanopore library prep from single wild-caught drosophilids v2
Authors: Y Kim Bernard
Published: May 2022

We have been assembling the genomes of many Drosophila species. This protocol fork allows for the sequencing of single wild-caught flies using Nanopore MinION sequencers, in contrast to our previous protocol that originally used gDNA extracted from tens or hundreds of flies from an inbred line. While read lengths are not nearly close to what we were seeing before, libraries range from 2-10kb read N50 and typically produce genome assemblies with contig N50 >1 Mbp. The protocol for ultra-long read sequencing from large pools of flies can be found here: dx.doi.org/10.17504/protocols.io.bdfqi3mw For now, please cite the original genomes paper if you use this protocol: https://doi.org/10.7554/eLife.66405 An update that includes many single wild-caught fly genomes is in progress.
Complex I activity assay v1
Authors: José Pérez J. María , not provided michela.deleidi
Published: May 2022

This protocol describes the complex I activity assay.
Single cell analysis of iPSC-derived midbrain organoids v1
Authors: José Pérez J. María , not provided michela.deleidi
Published: May 2022

The following script was used for analysis of gene corrected (GC) versus GBA1 mutant (MUT) midbrain organoids. The purpose was to combine, filter, integrate, and identify clusters and differentially expressed genes sets.
Metagenomic extraction of high molecular weight plankton from filters v1
Authors: Vacherie Benoît , Labadie Karine
Published: May 2022

Filtration and extraction protocol of plankton metagenomic samples to obtain high molecular weight DNA for sequencing on Minion nanopore. The protocol describes the following steps: - filtration of the water on filters of different porosity - Recovery and grinding of organisms - HMW extraction - Results
Endoglycosidase H digestion of GCase v1
Authors: Smith Laura
Published: May 2022

The N-linked glycoprotein profile of GCase trapped in the ER makes it susceptible to digestion by the glycosidase EndoH. Western blotting of GCase following EndoH digestion will yield an additional lower molecular weight band that corresponds to the GCase that is trapped in the ER.
Lipid droplet visualisation in cultured cells using BODIPY 493/503 stain v1
Authors: Smith Laura
Published: May 2022

Lipid droplets are organelles involved in intracellular lipid homeostasis, as well as playing key roles in a variety of cellular functions including cellular signalling, metabolic disease and inflammation. BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene) is a fatty acid-conjugated fluorescent probe that has been shown to successfully visualise the localisation and dynamics of lipid droplets in cells. Analysis can be performed on cells cultured in complete growth medium or in cells following lipid loading with oleic acid (OA) after over-night starvation in OptiMEM. OA is a potent inducer of lipid droplet formation.
X-tremeGENE™ HP DNA Transfection Reagent Protocol for transfection of SH-SY5Y cells v1
Authors: Smith Laura
Published: May 2022

SH-SY5Y cells were transfected with a pcDNA3.1 vector plasmid. Stable transfection was performed using the XtremeGENE reagent for 72 hours, and 400 µg/ml G418 antibiotics used as the selection marker . Colonies were selected and expanded for routine culture in growth media supplemented with G418.
MitoGFP Generation and Transduction v1
Authors: MLC Choi Minee-Liane
Published: May 2022

This protocol details the packaging of plasmids that are on a lentivirus backbone into a lentiviral construct, as well as cell transduction.
Patch-Seq Internal Solution with Biocytin v4
Authors: Institute for Brain Science Allen
Published: May 2022

This SOP provides instruction to prepare Internal Solution which is used for patch clamp electrophysiology, modified for cleanliness and mRNA capture and sustainability. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Liposomes v1
Authors: Sciences BOC
Published: May 2022

Liposomes are spherical vesicles with internal cavities and composed of one or more phospholipid bilayers. Their structural components are phospholipids or synthetic amphiphilic combined with cholesterol and other sterols, which can affect membrane permeability. Liposomes can encapsulate hydrophilic or lipophilic molecules. Their applications are now well established in various fields such as medicine, biomolecules, and gene delivery, and have been studied as DDS (drug delivery system) to enhance treatment safety and efficacy of the agent.
[RETRACTED] Titan XL Male Enhancement Review - Increase The Size Of Erections And Penis v1
Authors: not provided stashkevich
Published: May 2022

[RETRACTED]

Titan XL Male Enhancement is a nutritional supplement sold online through titanxl.com. The supplement is marketed to men who want to increase the length of their penis through natural methods permanently.According to titanxl.
RUN 001 v1
Authors: not provided maria
Published: May 2022

test
UNLISTED PROTOCOL 001 v1
Authors: not provided rober
Published: May 2022

test
[RETRACTED] Bagaimana Caranya Menang Slot Pulsa Dengan Mudah Di Megalsoto v1
Authors: marsuky umar
Published: May 2022

[RETRACTED]

a
PNA Oligomer Synthesis v1
Authors: Miller Cathy
Published: May 2022

PNA is a pseudo-peptide backbone instead of the ribonucleic acid-phosphate backbone, retaining the original 4 bases. Nielsen et al in Rose Laboratory of the University of Copenhagen used a simple computer DNA model to successfully design and synthesize PNA and PNA in 1991. The synthesis of PNA is first to synthesize the monomers, and then oligomerize the PNA monomers according to the standard peptide synthesis method. The structural unit of PNA is modified amino acid, so it can be prepared in large quantities by peptide synthesis, and it can also be prepared by chemical synthesis by attaching functional groups to the N-terminus or C-terminus. PNA oligomers are prepared from protected PNA monomers by solid-phase synthesis, and their protection strategies, condensation conditions, deprotection methods and purification methods are similar to those of peptide synthesis. Creative Peptidesteam has accumulated rich experience in PNA chemistry, we can provide two chemical synthesis methods to synthesize PNA oligomers, including Boc chemistry and Fmoc chemistry. The entire synthesis process will be implemented in accordance with strict quality control protocols, and we are committed to synthesizing high-quality, high-precision, and high-activity PNA products to meet customer expectations.
Cell Penetrating Peptide Design and Synthesis v1
Authors: Miller Cathy
Published: May 2022

Cell penetrating peptides (CPPs), also known as protein transduction domains (PTDs) or membrane transduction peptides (MTPs), are small molecular peptides that consist of 5-30 amino acid residues, which not only penetrate the cell membrane by themselves, but also carry a variety of exogenous substances such as peptides, proteins, nucleic acids and nanoparticles in a covalent or non-covalent binding manner into cells. CPPs have a wide range of transmembrane targets and can enter a variety of cell types, even through the blood-brain barrier when appliedin vivo. As an effective bioactive molecular intracellular transport tool, CPPs have broad application prospects in cell biology and cellular immunology, especially in the fields of drug development, gene biotherapy and tumor targeted therapy. Creative Peptides has focused on the design and synthesis of peptides for many years. With state-of-the-art equipment and experienced chemists, we can provide customers with GMP-grade cell-penetrating peptide synthesis services such as HIV-TAT and Oligo-Arg, etc. At the same time, we can also design new cell penetrating peptides with unsuitable or non-natural amino acids, peptide branches and other modifications according to customer needs to design suitable cell penetrating peptides to meet the research needs of customers. Creative Peptides offers a comprehensive series of cell-penetrating peptides complying with specific purities, scales and modifications for pharmaceutical and biochemical applications.
PNA-functional Ligand Conjugates Synthesis v1
Authors: Miller Cathy
Published: May 2022

In recent years, the development of PNA into genetic drugs has been one of the research hotspots. However, the biggest obstacle leading to the poor drug ability of PNA is still the problem of cell transport. Covalently linking PNA with a suitable functional ligand with pharmacological activity is a successful attempt in the development of PNA drugs. The successful design of PNA-functional ligand conjugates not only improves the cellular uptake of PNA, but also introduces functional ligand compounds into cells to achieve dual therapeutic effects of PNA and functional ligands. In this conjugate, the ligand not only has the function of assisting the transport, but also exerts its own pharmacological activity. At Creative Peptides, we always pay attention to the needs of our customers and recognize that careful planning and perfect execution are the key factors for success. Therefore, with our experience and our expertise in the PNA field, for each problem, we will form a professional development team to help you choose the process and technology that meets your research goals.
Node Propagation of Switchgrass v1
Authors: Brennan Darlene , Lowry David
Published: May 2022

This protocol can be used to clonally propagate switchgrass under sterile conditions in six weeks. It describes how to prepare and propagate the nodes on an MS-based media. These shoots are ideal for experiments that would require pest and pathogen-free switchgrass clones, such as microbiome studies.
Decisions and mechanisms of intergroup bias in children’s third-party punishment v1
Authors: Luz Gonzalez-Gadea Maria
Published: May 2022

Children tend to punish norm transgressions, even when they are mere external observers—a phenomenon known as Third-Party Punishment (TPP). This behavior is influenced by intergroup bias, as children unevenly punish ingroup and outgroup members. Here, we explored the influence of intergroup bias in TPP decisions in children between six and 11 years of age (N=124) using costly versus non-costly decisions while measuring response times as a proxy of the mechanisms underlying intergroup bias in TPP. Our results showed that children exhibited two types of intergroup biases: (i) an ingroup policing bias, as they preferentially punished selfish behavior from ingroup members, and (ii) an ingroup protectionism bias, as they were more willing to punish selfishness directed at ingroup members than outgroup members. We observed different developmental trajectories and mechanisms associated with these biases. First, while ingroup protectionism was present in all children, ingroup policing decreases with age and it was absent in children between 10 and 11 years of age. Second, both intergroup biases were associated with deliberative/conflictive processes. These results shed light on the mechanisms that underlie the development of intergroup bias in TPP decisions.
[RETRACTED] PNA Oligomer Synthesis v1
Authors: Miller Cathy
Published: May 2022

[RETRACTED]

PNA is a pseudo-peptide backbone instead of the ribonucleic acid-phosphate backbone, retaining the original 4 bases. Nielsen et al in Rose Laboratory of the University of Copenhagen used a simple computer DNA model to successfully design and synthesize PNA and PNA in 1991. The synthesis of PNA is first to synthesize the monomers, and then oligomerize the PNA monomers according to the standard peptide synthesis method. The structural unit of PNA is modified amino acid, so it can be prepared in large quantities by peptide synthesis, and it can also be prepared by chemical synthesis by attaching functional groups to the N-terminus or C-terminus. PNA oligomers are prepared from protected PNA monomers by solid-phase synthesis, and their protection strategies, condensation conditions, deprotection methods and purification methods are similar to those of peptide synthesis. Creative Peptidesteam has accumulated rich experience in PNA chemistry, we can provide two chemical synthesis methods to synthesize PNA oligomers, including Boc chemistry and Fmoc chemistry. The entire synthesis process will be implemented in accordance with strict quality control protocols, and we are committed to synthesizing high-quality, high-precision, and high-activity PNA products to meet customer expectations.
LRRK2RCKW single molecule kinesin motility assays v1
Authors: Salogiannis John
Published: May 2022

This protocol is about LRRK2RCKW single molecule kinesin motility assays.
DifferentiationNPCsto Dopaminergic/Midbrain Neurons v1
Authors: not provided michela.deleidi
Published: May 2022

This protocol details methods for differentiation of NPCs to Dopaminergic/Midbrain Neurons.
Microbiome and eDNA sampling of water using Sterivex filters v1
Authors: S Read Daniel
Published: May 2022

Protocol for field based sampling of bacterial biomass or environmental DNA (eDNA) using Sterivex filters and syringes.
WU sn-prep Protocol for Solid Tumors - snRNA protocol v2.7 v1
Authors: Caravan Wagma , Jayasinghe Reyka , Naser Al Deen Nataly
Published: May 2022

WU sn-prep Protocol for Solid Tumors -snRNA protocol v2.7
Symbiont density quantification in live Aiptasia v2
Authors: Maruyama Shumpei , Weis Lab OSU , Weis Virginia
Published: May 2022

This protocol is designed for quantifying symbiont density during the onset ofsymbiosis and early proliferation. Other techniques should be used for quantifying high symbiont density.
SDS-PAGE v1
Authors: Bird Anna , Gandini Chiara
Published: May 2022

SDS-PAGE gels are used to visualize proteins. This protocol describes how to prepare all the buffers required for casting and running SDS-PAGE gels, as well as how to prepare whole cell samples.
QIAGEN DNeasy Power Water SOP v1
Authors: Ottesen Andrea , Kocurek Brandon
Published: May 2022

QIAGEN DNeasy PowerWater SOP Purpose: For the isolation of genomic DNA from filter water samples, inculding turbid water Introduction: The DNeasy PowerWater Kit can isolate genomic DNA from a variety of filtered water samples. Utilizing Inhibitor Removal Technology® (IRT), even water containing heavy amounts of contaminants can be processed to provide DNA of high quality and yield. The DNeasy PowerWater Kit can isolate DNA equally well from any commonly used type of filter membrane. Purified DNA is ready to use in a final elution volume of 100 μl. Principle & Procedure: The DNeasy PowerWater Kit starts with the filtration of a water sample onto a filter membrane. Filter membranes may be user supplied. The membrane is then added to our special 5 ml bead beating tube containing a unique bead mix. Rapid and thorough lysis occurs through vortexing in a specially formulated lysis buffer that enhances the isolation of microorganisms from filter membranes. After the protein and inhibitor removal steps, total genomic DNA is captured on an MB Spin Column. High-quality DNA is then washed and eluted from the MB Spin Column membrane for use in downstream applications including PCR and qPCR.
[RETRACTED] PNA Synthesis v1
Authors: Miller Cathy
Published: May 2022

[RETRACTED]

Peptide nucleic acid is one of the more important oligonucleotide mimics. Although PNA has a significant change in structure relative to oligonucleotides, the binding between PNA and complementary nucleic acids still follows the principle of base complementary pairing, it even has higher affinity than natural nucleotides, and has stronger resistance to degradation by nucleases and proteolytic enzymes. Researchers have designed and synthesized PNA with various structures to optimize its biological stability and availability, target binding characteristics, and pharmacokinetic characteristics. We provide customers with various types of PNA conjugates and tailor-made PNA products for customer projects to achieve scientific research purposes. Our product analysis service will help our customers perform product analysis without worrying about poor product quality.
[RETRACTED] Custom Peptide Design v1
Authors: Miller Cathy
Published: May 2022

[RETRACTED]

Interested in the use of biologically active proteins and peptides as potential therapeutic agents has grown dramatically in recent years. Although many meaningful studies can be performed, it is important to consider several factors such as the length of sequence, amino acid residues and the sequence of peptides, all of which will influence whether correct assembly and purification are feasible. If you would like Creative Peptides to help you in designing your peptide, our technical support department can assist you.
[RETRACTED] PNA/DNA Chimera Synthesis v1
Authors: Miller Cathy
Published: May 2022

[RETRACTED]

Pure PNA has significant binding properties, but it has completely different properties from DNA and RNA. For example, it cannot be recognized as a substrate when it interacts with nucleic acid modifying enzymes. On the one hand, this is an advantage, because it makes PNA very stable for the degradation of nucleases; but on the other hand, it also limits certain applications of PNA, such as not being used as a substrate for DNA polymerase, DNA kinase or DNA ligase. In one molecule, the combination of PNA and DNA can produce PNA/DNA chimeras with new properties. This substance significantly improves the solubility of PNA in aqueous solutions, PNA/DNA chimera can be analyzed and purified by PAGE and ion exchange chromatography because the DNA part is negatively charged. Interestingly, PNA/DNA chimeras also have biological functions, such as being used as primers for DNA polymerases or binding to RNA to mediate the dissociation of RNase H. Finally, the study of PNA/DNA chimeras can provide useful information about the structural and functional properties of DNA and RNA themselves. Creative Peptides provides PNA/DNA chimera related services, we can provide design and synthesis integrated services, and committed to helping you solve synthesis problems and customize high-stability, multi-type PNA/DNA chimera products.
[RETRACTED] Non-cleavable Linkers v1
Authors: Sciences BOC
Published: May 2022

[RETRACTED]

Non-cleavable linkers require mAb degradation within lysosomes after ADC internalization to release the active drug. BOC Sciencesprovides synthetic non-cleavable linker products with a wide range of scales, purities, and delivery time options to meet your research needs. We ensure that each custom linker product is multiple-checked for quality via both mass spectrometry (MS) and high-performance liquid chromatography (HPLC) analyses during linker purification and quality control (QC) procedures after each step.
[RETRACTED] PNA-functional Ligand Conjugates Synthesis v1
Authors: Miller Cathy
Published: May 2022

[RETRACTED]

In recent years, the development of PNA into genetic drugs has been one of the research hotspots. However, the biggest obstacle leading to the poor drug ability of PNA is still the problem of cell transport. Covalently linking PNA with a suitable functional ligand with pharmacological activity is a successful attempt in the development of PNA drugs. The successful design of PNA-functional ligand conjugates not only improves the cellular uptake of PNA, but also introduces functional ligand compounds into cells to achieve dual therapeutic effects of PNA and functional ligands. In this conjugate, the ligand not only has the function of assisting the transport, but also exerts its own pharmacological activity. At Creative Peptides, we always pay attention to the needs of our customers and recognize that careful planning and perfect execution are the key factors for success. Therefore, with our experience and our expertise in the PNA field, for each problem, we will form a professional development team to help you choose the process and technology that meets your research goals.
[RETRACTED] Labelled Amino Acids v1
Authors: Sciences BOC
Published: May 2022

[RETRACTED]

Isotope-labeled amino acids refer to the replacement of single or multiple atoms of the amino acid with isotopes, which can be radioactive isotopes or stable isotopes. Basically, isotope-labeled amino acids have identical structures and properties as non-labeled amino acids. The trends of isotopes-labeled amino acids can be observed by analytical methods such as mass spectrometry and nuclear magnetic resonance. Therefore, isotope-labeled amino acids serve as safe, effective, and convenient tracer tools for scientific research in medicine, biology, pharmacy, chemistry,etc. In the field of medicine: Isotope-labeled amino acids can be used for the clinical diagnosis of various diseases. Labeled amino acids can be used as tracers to track the metabolic processes of amino acids and proteins in different physiological and pathological conditions, which allows the exploration of disease mechanism and effective ways to prevent and treat diseases. In the field of biology: Isotope-labeled amino acids are used as starting materials to study the biosynthetic pathways and mechanisms of natural products in animals, plants, and microorganisms by analyzing the abundance of labeled elements in products. In the field of pharmacy: Isotope-labeled amino acids are critical tools for the development of new drugs, and are used to determine the transfer, transformation, efficacy, mechanism of action, toxicity, and side effects of drugsin vivo. In the field of chemistry: Isotope-labeled amino acids can be used as tracers to explore chemical reaction mechanisms and as raw materials for the synthesis of other labeled products, such as labeled polypeptides.
[RETRACTED] Cell Penetrating Peptide Design and Synthesis v1
Authors: Miller Cathy
Published: May 2022

[RETRACTED]

Cell penetrating peptides (CPPs), also known as protein transduction domains (PTDs) or membrane transduction peptides (MTPs), are small molecular peptides that consist of 5-30 amino acid residues, which not only penetrate the cell membrane by themselves, but also carry a variety of exogenous substances such as peptides, proteins, nucleic acids and nanoparticles in a covalent or non-covalent binding manner into cells. CPPs have a wide range of transmembrane targets and can enter a variety of cell types, even through the blood-brain barrier when appliedin vivo. As an effective bioactive molecular intracellular transport tool, CPPs have broad application prospects in cell biology and cellular immunology, especially in the fields of drug development, gene biotherapy and tumor targeted therapy. Creative Peptides has focused on the design and synthesis of peptides for many years. With state-of-the-art equipment and experienced chemists, we can provide customers with GMP-grade cell-penetrating peptide synthesis services such as HIV-TAT and Oligo-Arg, etc. At the same time, we can also design new cell penetrating peptides with unsuitable or non-natural amino acids, peptide branches and other modifications according to customer needs to design suitable cell penetrating peptides to meet the research needs of customers. Creative Peptides offers a comprehensive series of cell-penetrating peptides complying with specific purities, scales and modifications for pharmaceutical and biochemical applications.
An Examination of the Use of Subtitles in Learning L2 Collocations
Authors: Xie Han
Published: May 2022

Exposure to audiovisual input has been recognised as an engaging approach for implicit vocabulary learning. Recent studies have compared the effectiveness of different types of subtitling in enhancing viewers' learning gains in single word vocabulary, but research on multiword vocabulary , especially collocations, remains limited. The present study aims to examine which type of subtitling (i.e., English–Chinese subtitles or English-only subtitles) is more effective in L2 implicit collocation learning, and it attempts to identify Chinese L2 learners’ learning difficulties when acquiring collocations. Experiments and interviews were conducted with 66 Chinese undergraduates to investigate their acquisition, in terms of both form recognition and meaning recall. Results indicate that compared with English-only subtitles, the effectiveness of English–Chinese subtitles in enhancing the acquisition of L2 collocation meaning is more significant, although English-only subtitles can be more helpful in reinforcing collocation forms. The results of the interviews also suggest that participants are more likely to encounter difficulties with contextually marked phrasal verbs and idioms, and affective filters should be considered when analysing individual differences. The findings support the use of L1 in subtitles to improve the learning efficiency of vocabulary meaning, as well as to help teachers identify the sources of students’ learning difficulties.