bluuebunny/crossref_metadata_2025 huggingface dataset:
A nem alkoholos zsírmájbetegség
Authors: Baffy György
Published: March 2022

A nem alkoholos zsírmájbetegség becslések szerint az emberiség 25%-át érinti, gyakran társul más anyagcserezavarokkal és nagymértékben heterogén a lefolyása. Steatosis formájában csekély a súlyosabb májkárosodás valószínűsége, míg a steatohepatitisszel járó esetek harmada cirrhosis felé halad, és súlyos szövődményként primer májrák kialakulásához is vezethet. Ennek valószínűsége együtt növekszik a májfibrosis mértékével, valamint a 2-es típusú cukorbetegség, esetleg két vagy több anyagcsere-rizikófaktor jelenléte esetén. A betegség azonban, a májkárosodás súlyosságától függetlenül, fokozza a cardiovascularis betegségek, a diabetes és a malignus daganatok iránti fogékonyságot, és ennek megfelelően rövidíti a várható élettartamot. Mindezek ellenére az egészségügyi szakemberek és betegek ismeretei a kórképpel kapcsolatosan meglehetősen korlátozottak. Ebben valószínűleg szerepet játszik az is, hogy a nem alkoholos zsírmájbetegségnek egyelőre nincs általánosan elfogadott gyógyszeres terápiája. Tekintve, hogy a betegség alapvető oka leggyakrabban a kalóriatöbblet, az elsődleges kezelés lényege az életmódváltoztatás, a mediterrán diéta, az alkoholfogyasztás kerülése és a rendszeres fizikai aktivitás. Szélesebb körű ismeretterjesztés segítheti mindennek felismerését és elfogadását az alapellátásban, párhuzamosan a máj fibrosis kétlépcsős szűrésének bevezetésével, amely a fokozott rizikójú betegek hatékony felismerését és ellátását célozza.
Çevre Bilinci Dinamikleri Çevresel Tutumda Bir Farklılık Yaratır mı? Kırklareli Üniversitesi Örneği
Authors: İBA GÜRSOY Sezin , HOCAOĞLU BAHADIR Neriman , YALÇIN Esin Cumhur
Published: March 2022

Bu çalışma üniversite öğrencilerinin çevreye yönelik tutumlarını etkileyen demografik özellikleri ve çevresel bilinç dinamiklerinin çevresel tutumları üzerinde yarattığı farklılığı ortaya koymayı amaçlamaktadır. Bu amaç doğrultusunda, araştırmanın analiz kısmında, Kırklareli Üniversitesi İktisadi ve İdari Bilimler Fakültesi öğrencilerine Şama (2003) tarafından geliştirilen 21 maddelik çevresel tutum ölçeği uygulanmıştır. Doğrulayıcı ve açıklayıcı faktör analizi sonuçlarına göre mevcut ölçekte yer alan maddeler geçerlilik ve güvenilirlik analiz sonuçlarına göre 10 maddeye indirgenmiştir. 438 öğrencilik örneklemden elde edilen bulgular doğrultusunda bazı çevresel bilinç dinamiklerinin (çevre sorunlarına olan ilgi, çevre derslerinin gerekliliği, çevre kuruluşlarının etkinliklerine katılım ve çevre kuruluşlarına verilen maddi destek) öğrencilerin çevresel tutumlarında farklılık oluşturduğu tespit edilmiştir. Bu çerçevede çalışmanın hem yeni bir ölçek geliştirilmesi hem de çevresel bilinç dinamiklerinin oluşturulmasına katkı sağlaması açısından yöntemsel ve kavramsal olarak yazın için önem arz edeceği düşünülmektedir.
Evaluasi Usability Website dengan Website Usability Evaluation tool dan Importance-Performance Analysis
Authors: Simatupang Hovely Wahyu , Riskiana Rosa Reska , Widowati Sri , Alitu Aqila Fitri
Published: March 2022

Pariwisata merupakan program unggulan pemerintah khususnya pemerintah daerah. Dalam menyebarkan informasi dan meningkatkan pelayanan publik pada bidang pariwisata, Dinas Kebudayaan dan Pariwisata Kota Bandung telah menerapkan sistem informasi e-government berbasis website. Namun, terdapat berbagai keluhan dari pengguna website Dinas Kebudayaan dan Pariwisata Kota Bandung yang menyebutkan bahwa informasi pada website sulit ditemukan, respon website cukup lama, dan lain sebagainya. Maka dari itu, perlu dilakukan evaluasi usability pada website tersebut untuk meningkatkan kemudahan dan efisiensi saat digunakan. Untuk mengevaluasi usability website digunakan metode pengukuran dari sudut pandang pengguna, yaitu Website Usability Evaluation Tool (WEBUSE). Hasil evaluasi WEBUSE diukur menggunakan metode Importance-Performance Analysis (IPA) untuk mengetahui perbaikan-perbaikan yang perlu diprioritaskan. Hasil pengukuran menunjukkan usability website saat ini berada pada level Moderate dengan nilai 0,56, sedangkan website perbaikan berada pada level Good denga nilai 0.78. Hasil performance (kinerja) website saat ini memiliki nilai rata-rata 3,25, sedangkan performance website perbaikan memiliki nilai rata-rata 4,10. Sebelas permasalahan website saat ini yang masuk ke dalam kuadran concentrate here meningkat pada website perbaikan menjadi kuadran keep up the good work.
The First ‘British’ Colony in the Americas: Inter-kingdom Cooperation and Stuart-British Ideology in the Colonisation of Newfoundland, 1616–1640
Authors: Wagner Joseph
Published: March 2022

The accession of James VI, the Stewart (or Stuart) King of Scots, to the thrones of England and Ireland in 1603 renewed debates about ‘Britishness’. Many of the king’s attempts to popularise and codify his version of the concept were unsuccessful. His vision for closer political union between England and Scotland did not come to pass until 1707 and most historians attribute the formation of British identity to the eighteenth century. Most influentially, Linda Colley has argued that British identity was forged in the crucible of eighteenth-century empire-building as English, Scottish, and Welsh people lived, worked, and fought together across the globe in defence of shared values. English, Scottish, Welsh, and Irish people and interests likewise coalesced in the pursuit of empire in the early seventeenth century. They cooperated in attempts to colonise Newfoundland and explicitly promoted the project as British. The Newfoundland example shows that James’ British vision had some success. This article examines the project’s ‘Britishness’ and argues that the 1603 union of the crowns’ role in the formation of British identity and its impact on overseas expansion requires additional attention.
UZAKTAN VE YÜZ YÜZE EĞİTİM ALAN HEMŞİRELİK BÖLÜMÜ ÖĞRENCİLERİNDE UYKU KALİTESİ VE COVİD-19 KAYNAKLI ANKSİYETE İLİŞKİSİ
Authors: EFTELİ Elçin
Published: March 2022

Bu araştırmanın amacı; uzaktan ve yüz yüze eğitim alan öğrencilerin uyku kaliteleri ve Covid-19 kaynaklı anksiyete düzeyleri arasındaki ilişkiyi belirlemektir. Araştırma; hemşirelik bölümünde uzaktan ve yüz yüze eğitim alan 116 öğrenci ile yürütülmüştür. Verilerin toplanmasında; “Pittsburg Uyku Kalitesi İndeksi ” ve “Koronavirüs Anksiyete Ölçeği Kısa Formu” kullanılmıştır. Araştırma sonucunda, öğrencilerin düşük uyku kalitesine sahip oldukları belirlenmiştir. Öğrencilerin Covid-19 kaynaklı anksiyeteleri düşük düzeyde bulunmuştur. Yakınını Covid-19 yüzünden kaybedenlerin anksiyete düzeyleri daha yüksektir. Yaş ve cinsiyetin uyku kalitesini ve anksiyete seviyesini etkilemediği belirlenmiştir. Eğitimin yüz yüze ya da online olmasının uyku kalitesi ve anksiyete üzerinde etkisinin bulunmadığı belirlenmiştir.
Analisis Kualitas Jaringan Internet Provider Telekomunikasi Dengan Menggunakan Parameter Quality of Service (QoS) Di Kota Kendari
Authors: Setiawan Edwin , Pagiling Luther , Nur Muh Nadzirin Anshari
Published: March 2022

Masih banyak para pelanggan yang belum mengetahui kualitas jaringan internet Provider telekomunikasi di berbagai daerah di Kota Kendari. Oleh karena itu, dilakukan penelitian ini yang bertujuan untuk mengetahui kualitas jaringan internet Provider telekomunikasi di Kota Kendari. Dalam menentukan kualitas jaringan internet di setiap Providerr menggunakan sebuah parameter yaitu parameter Quality of Service (QoS). Terdapat empat parameter Quality of Service yang dibahas adalah througputt, packet loss, delay dan jitter. Providerr telekomunikasi yang menjadi objek penelitian adalah kartu Provider telkomsel, XL dan 3 tri. Dalam menetukan kualifikasi kualitas jaringan internet menggunakan standarisasi menurut Telecommunications and Internet Protocol Harmonization Over Network (THIPON). Berdasarkan perhitungan yang telah dilakukan menghasilkan nilai througputt yang lebih baik pada Provider XL dengan nilai sebesar 1,52012 kbps. Untuk nilai packet loss menghasilkan nilai yang sama untuk ketiga Provider sebesar 0%. Nilai delay yang lebih baik terdapat pada Provider 3 tri dengan nilai sebesar 1,15259 sekon. Untuk nilai jitter yang lebih baik terdapat pada Provider 3 tri dengan nilai sebesar 1,1033 sekon. 2. Hasil perhitungan indeks nilai parameter Quality of Service (QoS) menunjukkan bahwa indeks nilai dari ketiga Provider menghasilkan indeks nilai yang sama, dengan indek nilai sebesar 3,33 dan termasuk dalam kategori bagus..
Penerapan Metode Waterfall Pada Pengendali Penerangan Ruangan Berbasis Fuzzy Logic
Authors: Yuliana Dian Efytra
Published: March 2022

Setiap rumah memerlukan ruangan yang baik dan nyaman, salah satu aspek yang mempengaruhi kenyamanan sebuah ruangan adalah pada sistem pencahayaanya. Pada saat ini masih banyak digunakan sistem pencahayaan manual dengan prinsip on-off. Sebuah ruangan yang pencahayaannya terpengaruh oleh cahaya alam sekitar dan mempunyai system pencahayaan manual bisa mengurangi tingkat kenyamanan ruangan karena kecerahan ruangan tidak bisa diatur secara otomatis sesuai kebutuhan. Sehingga ruangan bisa menjadi terlalu terang atau terlalu redup. Hal ini dapat mengakibatkan gangguan kesehatan dan mengganggu efisiensi kerja bagi penggunanya. Oleh karena itu diperlukan sistem pencahayaan yang dapat mengkondisikan intensitas penerangan sesuai dengan kebutuhan. Alat ini bekerja berdasarkan hasil pembacaan dari LDR yang masuk ke arduino melalui port ADC. Hasil pembacaan ADC dijadikan inputan kendali fuzzy pada progam arduino. Hasil defuzzifikasi progam arduino digunakan untuk mengatur besarnya pwm yang akan diterima oleh rangkaian solid state relay. Besarnya pwm yang terima oleh rangkaian solid state relay berbanding lurus dengan tegangan yang diterima oleh lampu, sehingga besar intensitas cahaya lampu dapat diatur sesuai dengan pwm dari Arduino. LCD  digunakan untuk menampilkan hasil defuzzifikasi progam. Hasil system yang di terapkan menggunakan inferensi fuzzy logic metode sugeno ordo nol dengan metode min dalam proses evaluasi aturannya mampu mempresentasikan nilai dari sebuah intensitas cahaya sekitar. Kata kunci — LDR, arduino, fuzzy logic, solid state relay, LCD.
SIMPLE TECHNIQUES TO ACTIVATE SCHEMATA
Authors: Hijaz Ahmad , Lio Asrun , Kamaluddin Kamaluddin
Published: March 2022

Activating schemata to improve reading comprehension has been acknowledged and well accepted in academic world for decades, however many teachers are still recultant to spare time in this area. It’s believed that activating schemata requires heavy preparation and certain additional skills aside from their existing ones.This article offers four simple techniques that can help activating students’ schemata which in turn allow them perform better in reading comprehension tasks. In addition, the techniques can be performed with minimum equipments and less time consumption
ANALISIS KINERJA KEUANGAN DAN KEMAMPUAN KEUANGAN PEMERINTAH DAERAH DALAM PELAKSANAAN OTONOMI DAERAH DI PROVINSI SULAWESI TENGGARA
Authors: Mustarim Israwati , Rosnawintang Rosnawintang , Akib Mulyati
Published: March 2022

This study aims to analyze financial performance and financial capacity in implementing regional autonomy. The object of this research is financial performance and financial capacity in the implementation of regional autonomy in 2011 to 2017. The data collection techniques of this study describe, analyze and interpret the financial performance and financial capabilities of the Regency / City government of Southeast Sulawesi Province which are measured by calculating financial ratios based on reports realization of APBD for fiscal year 2011-2017 and presented in writing in a systematic manner. The results of this study found that financial performance is still not financially independent and is in an innovative relationship pattern, where external parties are still very much needed in government administration, the effectiveness of PAD is very good and indirect expenditure activities in parent regions are allocated more than 50 percent, while for autonomous regions direct expenditure is allocated more than 50 percent, financial capability measured by the degree of fiscal autonomy is in the very low category but the financial ability index is very good. Keywords: Regional Autonomy, Financial Ability, Financial Performance
ANALISIS KINERJA KOPERASI PEGAWAI NEGERI (KPN)
Authors: YENI YENI , Rosnawintang Rosnawintang , Nusantara Ambo Wonua
Published: March 2022

Penelitian ini bertujuan untuk menganalisis kinerja Koperasi Pegawai Negeri (KPN) Lingkup Pemerintah Provinsi Sulawesi Tenggara dan faktor yang mempengaruhi kinerja Koperasi Pegawai Negeri (KPN) Lingkup Pemerintah Provinsi Sulawesi Tenggara. Lokasi penelitian ini adalah dua Koperasi Pegawai Negeri Lingkup Pemerintah Provinsi  Sulawesi Tenggarayaitu Koperasi Takdir pada Dinas Koperasi dan UMKM dan KPN Niaga di Dinas Perindustrian dan Perdagangan Provinsi Sulawesi Tenggara. Hasil penelitian menemukan bahwa Kinerja Kinerja pada KPN Niaga (Dinas Perindustrian dan perdagangan Provinsi Sulawesi Tenggara) berada pada ketagori cukup baik, sementara kinerja KPN Takdir (Dinas Koperasi dan UMKM Provinsi Sulawesi Tenggara) dari keempat perspektif Balanced Scorecard berada pada kategori buruk dan Kinerja KPN lingkup Pemerintah provinsi Sulawesi Tenggara  dilihat dari empat aspek sebagai berikut a) aspek keuangan dipengaruhi oleh faktor kepemimpinan, kemampuan pengurus dan dukungan anggota, b) aspek pelanggan dipengaruhi oleh faktor kepemimpinan, kemampuan pengurus dan teknologi, c) aspek proses bisnis dipengaruhi oleh kepemimpinan, kemampuan pengurus, dukungan anggota dan teknologi dan d) aspek pembelajaran dan pertumnbuhan dipengaruhi oleh faktor kepemimpinan.  Kata Kunci : Kinerja , Koperasi Pegawai Negeri, Kemampuan penguirus koperasi
EFEKTIVITAS SIKLUS PENGELOLAAN BARANG MILIK NEGARA STUDI PADA UNIVERSITAS HALU OLEO KENDARI
Authors: Inthe Desryani Ayulin , Mas’ud Arifuddin , Saranani Fajar
Published: March 2022

Penelitian ini bertujuan untuk  Untuk mengkaji siklus pengelolaan BMN Negara dalam konteks Perencanaan, pengadaan, penggunaan, pemanfaatan, pengamanan dan pemeliharaan, penilaian, pemusnahan, penghapusan, pemindahtangan, penatausahaan, serta pembinaan dan pengawasan dalam pengelolaan BMN pada Universitas Halu Oleo.            Penelitian ini merupakan penelitian deskriptif kuantitatif dengan sumber data berasal dari wawancara dan kuesioner dan dokumen-dokumen terkait.  Teknik pengambilan sampel dalam penelitian ini menggunakan Purposive sampling, dengan teknik pengumpulan data wawancara, kuesioner, observasi dan dokumentasi.  Subjek penelitian adalah pejabat, staf pengelola, pengadministrasi Barang Milik Negara, serta operator SIMAK BMN pada Universitas Halu Oleo yang diwakili dengan 17 orang. Analisa data dimulai dengan tahap pengumpulan data, distribusi data, penyajian data, dan penarikan kesimpulan.           Hasil penelitian menunjukkan pengelolaan Barang Milik Negara belum sepenuhnya maksimal sesuai dengan peraturan yang berlaku; penatausahaan perlu ditingkatkan agar pencatatan kedalam aplikasi SIMAK BMN dapat dilakukan dengan baik; peningkatan pengawasan terhadap kegiatan pengelolaan BMN agar sesuai dengan Peraturan Pemerintah Nomor 27 tahun 2014.Kata Kunci :  Pengelolaan BMN, Siklus Pengelolaan
ANALISIS DETERMINAN FOREIGN DIRECT INVESTMENT (FDI) DI NEGARA-NEGARA ASEAN
Authors: M. Aspar Andi , Syarif Muh. , Rosnawintang Rosnawintang
Published: March 2022

Tujuan Penelitian ini adalah untuk menganalisis variabel makro yang mempengaruhimasuknya modal asing atau foreign direct investment ke negara ASEAN. Penelitian ini diukurdengan variabel ukuran pasar (GDP), nilai tukar, nilai impor dan suku bunga. Jenis data yangdigunakan dalam penelitian ini adalah data sekunder yang di akses melalui website kantorSekretariat ASEAN. Data tersebut menggunakan data time series tahunan dari tahun 2013-2017.Alat analisis yang digunakan adalah regresi data panel dengan bantuan aplikasi Eviews.Hasil penelitian ini yang sesuai dengan hipotesis terdapat 2, dimana variabel nilai impordan suku bunga berpengaruh positif terhadap Foreign Direct Investment. Sementara variabelukuran pasar (GDP) mempunyai pengaruh positif tidak signifikan terhadap FDI, begitu pulavariabel nilai tukar berpengaruh negatif dan tidak signifikan. Variabel suku bunga merupakanvariabel yang paling dominan dalam mempengaruhi masuknya investasi modal asing ke negaraASEAN.Kata Kunci: Foreign Direct Investment, Nilai Tukar, Nilai Impor, Suku Bunga
PENGARUH GAYA KEPEMIMPINAN DAN LINGKUNGAN KERJA TERHADAP KINERJA PEGAWAI TELEVISI REPUBLIK INDONESIA STASIUN SULAWESI TENGGARA
Authors: Marjan Marjan , Rosnawintang Rosnawintang , Husin Husin
Published: March 2022

Penelitian bertujuan untuk menguji dan menganalisis pengaruh gaya kepemimpinan dan lingkungan kerja terhadap kinerja pegawai. Studi pada TVRI Stasiun Sulawesi Tenggara. Penelitian ini menggunakan pendekatan kuantitatif. Populasi dan sampelnya adalah seluruh pegawai TVRI Stasiun Sulawesi Tenggara yang berjumlah 92 orang. Teknik pengambilan sampel menggunakan sampling jenuh atau sensus. Metode pengumpulan data menggunakan kuesioner. Sedangkan Analisis data menggunakan analisis statistik deskriptif dan uji kualitas data menggunakan uji reliabilitas dan uji validitas serta uji asumsi klasik. Analisis data diolah dengan bantuan software IBM SPSS 22.Hasil penelitian menunjukkan bahwa (1) gaya kepemimpinan berpengaruh signifikan terhadap kinerja pegawai, (2) lingkungan kerja berpengaruh signifikan terhadap kinerja pegawai, (3) pemberian insentif berpengaruh tidak signifikan terhadap kinerja pegawai.Kata kunci: Gaya Kepemimpinan, Lingkungan Kerja, Pemberian Insentif, Kinerja Pegawai
Pengaruh Self Assessment System dan Pelaporan Surat Pemberitahuan (SPT) Terhadap Kepatuhan Wajib Pajak Pada Usaha Mikro, Kecil, dan Menengah (UMKM) Kota Kendari
Authors: Rauf Dian Mayafaty , Awaluddin Ishak , Abdullah Muntu
Published: March 2022

Penelitian ini bertujuan untuk mengetahui Pengaruh Self Assessment System dan Pelaporan Surat Pemberitahuan (SPT) Terhadap Kepatuhan Wajib Pajak Pada Usaha Mikro, Kecil, dan Menengah (UMKM) Kota Kendari. Penelitian ini menggunakan data primer melalui kuesioner. Responden dalam penelitian ini adalah seluruh pelaku UMKM yang ada di Kota Kendari. Variabel dalam penelitian ini adalah Self Assessment System dan Pelaporan SPT sebagai variabel bebas, serta Kepatuhan Wajib Pajak sebagai variabel terikat.Analisis data yang digunakan dalam penelitian ini adalah tehnik analisis regresi linier berganda yang diolah melalui software IBM SPSS versi 21, yang dijelaskan dengan statistik deskriptif dan statistik inferensial. Berdasarkan hasil penelitian didapatkan hasil pengujian hipotesis menunjukkan bahwa secara parsial self assessment system berpengaruh positif dan signifikan terhadap kepatuhan wajib pajak dengan nilai probability 0.034 < 0.05, dan pelaporan SPT berpengaruh positif dan signifikan terhadap kepatuhan wajib pajak dengan nilai probability 0.000 < 0.05.Kata Kunci : Self Assessment System, Pelaporan SPT, Kepatuhan Wajib Pajak.
PENGARUH PENGELUARAN PEMERINTAH TERHADAP PASAR MATA UANG LUAR NEGERI DI INDONESIA: SUATU APLIKASI MODEL CCC-GARCH
Authors: Saidi La Ode , Rosnawintang Rosnawintang , Adam Pasrun , Rumbia Wali Aya
Published: March 2022

Penelitian ini bertujuan untuk menguji pengaruh pengeluaran pemerintah terhadap pasar mata uang luar negeri di Indonesia menggunakan model CCC-GARCH untuk menganalisi data runtun waktu kuartalan yang merentang dari 1985 hingga 2018. Hasil uji memberikan evidens empirik dimana kenaikan pengeluaran pemerintah menyebabkan kurs domestik tedepresiasi (atau kurs mata uang luar negeri terapresiasi). Hal ini menunjukkan bahwa pengeluaran pemerintah memiliki pengaruh terhadap pasar mata uang luar negeri.Kata Kunci: Kurs, model CCC-GARCH, pengeluaran pemerintah,
VERSIONS OF FUGLEDE-PUTNAM THEOREM ON p-w-HYPONORMAL OPERATORS
Authors: NASLI BAKIR Aissa
Published: March 2022

The aim of the article is the presentation of certain extensions of the famous
Fuglede-Putnam Theorem on the class of $p$-$w$-hyponormal operators, which generalize some results
proved by authors in \cite{Prasad}.
A Prognostic Model Based on mRNA Expression Analysis of Esophageal Squamous Cell Carcinoma
Authors: Liu Ke , Jiao Ye-Lin , Shen Liu-Qing , Chen Pan , Zhao Ying , Li Meng-Xiang , Gu Bian-Li , Lan Zi-Jun , Ruan Hao-Jie , Liu Qi-Wei , Xu Feng-Bo , Yuan Xiang , Qi Yi-Jun , Gao She-Gan
Published: March 2022

Background:The aim of this study was to identify prognostic markers for esophageal squamous cell carcinoma (ESCC) and build an effective prognostic nomogram for ESCC.

Methods:A total of 365 patients with ESCC from three medical centers were divided into four cohorts. In the discovery phase of the study, we analyzed transcriptional data from 179 cancer tissue samples and identified nine marker genes using edgeR and rbsurv packages. In the training phase, penalized Cox regression was used to select the best marker genes and clinical characteristics in the 179 samples. In the verification phase, these marker genes and clinical characteristics were verified by internal validation cohort (n = 58) and two external cohorts (n= 81,n= 105).

Results:We constructed and verified a nomogram model based on multiple clinicopathologic characteristics and gene expression of a patient cohort undergoing esophagectomy and adjuvant radiochemotherapy. The predictive accuracy for 4-year overall survival (OS) indicated by the C-index was 0.75 (95% CI, 0.72–0.78), which was statistically significantly higher than that of the American Joint Committee on Cancer (AJCC) seventh edition (0.65). Furthermore, we found two marker genes (TM9SF1, PDZK1IP) directly related to the OS of esophageal cancer.

Conclusion:The nomogram presented in this study can accurately and impersonally predict the prognosis of ESCC patients after partial resection of the esophagus. More research is required to determine whether it can be applied to other patient populations. Moreover, we found two marker genes directly related to the prognosis of ESCC, which will provide a basis for future research.
eIF3a Regulates Colorectal Cancer Metastasis via Translational Activation of RhoA and Cdc42
Authors: Mei Chao , Liu Chong , Gao Ying , Dai Wen-Ting , Zhang Wei , Li Xi , Liu Zhao-Qian
Published: March 2022

Tumor metastasis is the major cause of tumor relapse and cancer-associated mortality in colorectal cancer, leading to poor therapeutic responses and reduced survival. eIF3a was previously described as an oncogene. However, its role in colorectal cancer progression and metastasis has not yet been fully investigated. In this study, the expression specificity and predictive value of eIF3a were investigated in clinical samples. The effects of eIF3a on cell proliferation and migration were verified in vivo and in vitro, respectively. The underlying molecular mechanism was revealed by western blotting, immunofluorescence, RNA-binding protein immunoprecipitation, and dual-luciferase reporter gene assays. The results showed that eIF3a was significantly overexpressed in tumor tissues compared with adjacent normal tissues. High eIF3a expression was correlated with tumor metastasis and overall survival. Downregulation of eIF3a obviously inhibited the proliferation and motility of malignant cells in vitro and in vivo. Mechanistically, eIF3a regulates Cdc42 and RhoA expression at the translation level, which further affects pseudopodia formation and actin cytoskeleton remodeling. Taken together, eIF3a accelerates the acquisition of the migratory phenotype of cancer cells by activating Cdc42 and RhoA expression at the translational level. Our study identified eIF3a as a promising target for inhibiting colorectal cancer metastasis.
Facile and Sustainable Synthesis of Commendamide and its Analogues
Authors: Villano Rosaria , Tinto Francesco , Di Marzo Vincenzo
Published: March 2022

Commendamide, or N-(3-hydroxypalmitoyl)-glycine 1a, is a gut microbiota-derived bioactive metabolite, structurally similar to long-chain N-acyl-amino acids which belong to the complex lipid signaling system known as endocannabinoidome and play important roles in mammals through activation of, inter alia, G-protein-coupled receptors (GPCRs). In this work, we describe a simple, green and economic method for the preparation of commendamide 1a, a GPCR G2A/132 agonist. The developed protocol is general and could also be applied to the synthesis of deuterated commendamide 1b, as well as to other minor microbiota-derived metabolites, such as the analog 2.
A Transient Stability Assessment Model Based on Fault Severity Assignment
Authors: Lin Jianxin , Cai Rongbin , Zheng Zonghua
Published: March 2022

Maintaining accurate and fast transient stability is essential for safe operation of the power system. With the development of wide-area measurement system, machine learning–based transient stability assessment has become the trend. However, in realistic application of the power system, the impacts on evaluation rules between critical samples and noncritical samples are different. Thus, an improved cost-sensitive coefficient assignment method based on fault severity is proposed. First, the fault severity of each unstable sample is calculated. Then, the correction coefficient of the loss function of the unstable sample is linearized according to different fault severities. The closer the sample is to the critical case, the higher the cost coefficient is. Finally, the improved cost-sensitive method is combined with the deep learning model and tested in the IEEE-39 bus system. As shown in the results, the improved cost-sensitive method, which gives different correction coefficients to samples according to different fault severities, has better performance.
Simulation Study of Gas Explosion Propagation Law in Coal Mining Face With Different Ventilation Modes
Authors: Liu Jiajia , Zhang Yang , Chen Shouqi , Nie Zishuo , Yang Di
Published: March 2022

In ventilation working face, different ventilation way is one of the important factors affecting the propagation of gas explosion shock wave. In order to study the gas explosion shock wave propagation laws in coal mining faces with different ventilation methods, using Fluent simulation software, combined with pipeline gas explosion experiments and the actual situation of gas accumulation area explosion in the corner of 415 coal face in Chenjiashan coal mine, Shaanxi Province, and on the basis of building a three-dimensional mathematical and physical model, the gas explosion simulations under Y-type and W-type ventilation methods were carried out, respectively. The results are described as follows: (1) The attenuation trend of overpressure peak value of inlet roadway 1 and working face with Y-type ventilation conforms to the power function form. Intake airway 2 and return airway have the same peak overpressure attenuation trend, but the peak value of overpressure in return airway is generally 10.6% higher than that in intake airway 2, and its attenuation rate is a process from low to high and then to low. (2) The peak attenuation trend of overpressure in intake airways 1 and 2 with W-type ventilation conforms to the form of power function, and the peak attenuation trend of overpressure in return airway conforms to the form of exponential function. The peak value of overpressure decreases with the increase in the distance from the explosion source, and its attenuation rate decreases gradually. The research results have important theoretical significance for improving the prevention of gas explosion in coal mining face with different ventilation modes.
Identification, Characterization, and Expression of a PRQFVamide-Related Peptide in Cephalopod Sepiella japonica
Authors: Qiu Jia-yin , Zheng Li-bing , Chi Chang-feng
Published: March 2022

Neuropeptides, as neurotransmitters and neuromodulators, have a variety of physiological functions in the mollusk. Here, a PRQFVamide-related peptide gene was cloned from cuttlefish Sepiella japonica (designated as SjPRQFVRP, GenBank Accession No: OK999997). The full length of SjPRQFVRP is 1748 bp, including an open reading frame (ORF) of 738 bp encoding 245 amino acids. The putative precursor protein comprises one signal peptide and four different mature pentapeptides: fourteen copies of PMEFLamide, three copies of RMEFLamide, one copy of AMEFLamide and GMEFLamide. Multiple alignments showed SjPRQFVRP shared 71% identity with that of Octopus vulgaris and supported the phylogenetic analysis. The spatio-temporal expression pattern showed that SjPRQFVRP mRNA was widely expressed among the 13 tissues and primarily abundantly expressed in the brain and optic lobe during the whole development stage. In situ hybridization data indicated that SjPRQFVRP was detected in the vertical lobe, subvertical lobe, anterior basal lobe, anterior pedal lobe, and optic lobes of the brain. Subcellular localization analysis revealed that the SjPRQFVRP protein was localized in the cytoplasm of HEK293 cells. Collectively, the results will provide a foundation for further exploring the mechanism of SjPRQFVRP function in cephalopods.
Transport Structure of the South Atlantic Ocean Derived From a High-Resolution Numerical Model and Observations
Authors: Xu Xiaobiao , Chassignet Eric P. , Dong Shenfu , Baringer Molly O.
Published: March 2022

The South Atlantic Ocean plays an important role in the Atlantic meridional overturning circulation (AMOC), connecting it to the Indian and Pacific Oceans as part of the global overturning circulation system. Yet, there are still open questions regarding the relative importance of the warm water versus cold water sources in the upper limb of the AMOC and on the detailed circulation pathways of the North Atlantic Deep Water (NADW) in the lower limb. These questions are addressed using model outputs from a 60-year, eddying global ocean-sea ice simulation that are validated against observations. We find that the Pacific Antarctic Intermediate Water (AAIW) plays a role in setting the temperature and salinity properties of the water in the subtropical South Atlantic, but that the upper limb of the AMOC originates primarily from the warm Indian water through the Agulhas leakage (9.8 Sv of surface water + 3.5 of AAIW) and that only a relatively small contribution of 1.5 Sv colder, fresher AAIW originates from the Pacific Ocean. In the lower limb, the NADW flows southward as a deep western boundary current all the way to 45°S and then turns eastward to flow across the Mid-Atlantic Ridge near 42°S before leaving the Atlantic Ocean, although there is clockwise recirculation in the Brazil, Angola, and Cape Basins.
Case Report: Synchronous Removal and Implantation of Peritoneal Dialysis Catheter Using Bilateral Transversus Abdominis Plane Block
Authors: Jakšić Ante , Vujičić Božidar , Deša Diana , Gršković Antun , Vukelić Ivan , Španjol Josip , Rački Sanjin , Markić Dean
Published: March 2022

Background

Peritoneal dialysis (PD) surgery include PD catheter insertion and removal. Both procedures require the use of anesthesia. The end-stage renal disease (ESRD) patients usually have severe comorbidities. The general anesthesia, because of its negative systemic effect, should be omitted in this vulnerable group of the patients. Transversus abdominis plane (TAP) block as a newer method of regional anesthesia is a technique without systemic effect and recently started to be used in ESRD patients for PD catheter placement and/or removal. Here we report a patient in whom we for the first time simultaneously removed and implanted a PD catheter by using a bilateral transversus abdominis plane block.

Case Presentation

The patient was an 80-year-old man who was admitted for removal of malfunctioned PD catheter. Since the patient opted for staying on PD simultaneous implantation of catheter was planned. Because of his age and significant comorbidities, general anesthesia was avoided and bilateral TAP block become our option. In the same anesthesia, using bilateral TAP block, the old PD catheter was removed and a new one was implanted. Until now the patient is on regular PD without any complications.

Conclusion

The TAP block could be used as a primary anesthetic technique in ESRD patients for PD surgery even for synchronous removal and implantation of PD catheter.
Lateralized Changes in Language Associated Auditory and Somatosensory Cortices in Autism
Authors: Deemyad Tara
Published: March 2022

Lateralized specialization of the two cerebral hemispheres is a fundamental structural hallmark of the human brain and underlies many cognitive functions and behavioral abilities. In typical developing individuals the influence of handedness on performance of various sensory modalities and the cortical processing has been well recognized. Increasing evidence suggests that several neurodevelopmental and psychiatric disorders such as bipolar disorder, schizophrenia, and autism spectrum disorders (ASD) are associated with abnormal patterns of cerebral lateralization. Individuals with ASD exhibit abnormal structural and functional lateralization of circuits subserving motor, auditory, somatosensory, visual face processing, and language-related functions. Furthermore, a high prevalence of atypical handedness has been reported in ASD individuals. While the hemispheric dominance is also related to functions other than handedness, there is a clear relationship between handedness and language-related cortical dominance. This minireview summarizes these recent findings on asymmetry in somatosensory and auditory cortical structures associated with language processing in ASD. I will also discuss the importance of cortical dominance and interhemispheric disruption of balance between excitatory and inhibitory synapses as pathophysiological mechanisms in ASD.
Effects of Moringa oleifera in Two Independents Formulation and as Neuroprotective Agent Against Scopolamine-Induced Memory Impairment in Mice
Authors: Arozal Wawaimuli , Purwoningsih Emni , Lee Hee Jae , Barinda Agian Jeffilano , Munim Abdul
Published: March 2022

Background

The cognitive deficit has frequently been found in the elderly population. Several studies have shown that every single part of Moringa oleifera, including leaves, roots, and seeds, has abundant micronutrients, such as flavonoids, which improve the neurobehavioral capacity. However, herb parts that display optimal neuropharmacological properties remain unknown.

Objective

We investigate whether M. oleifera seed oil (MOO) or aqueous M. oleifera leaves extracts (MOEs) may ameliorate memory impairment in mice induced with scopolamine (Sco). Additionally, the phytochemical analyses of those two independent formulations were analyzed.

Methods

In this study, 2 ml/kg body weight (BW) of MOO and 500 mg/kg BW of MOE were orally administered to the mice for 28 days, followed by intraperitoneal injection of Sco (1 mg/kg) at the day 22–28 to induce cognitive impairment in those mice.

Results

The Sco group showed memory retention impairment represented by the Y-maze and novel object recognition tests, significant enhancement of acetylcholine esterase (AChE) activity in hippocampus tissue (p < 0.0001), and increased the level of total antioxidant capacity (TAOC) in serum. Interestingly, the Sco-induced memory defect was improved and completely blunted the AChE exacerbation in Sco+MOO-treated mice (p < 0.0001), although the TAOC level was comparable among the groups. Mechanistically, both tropomyosin receptor kinase B (TrkB), as a brain-derived neurotrophic factor-receptor, and nuclear factor-kappa-light-chain-enhancer of activated B cells (NF-κB) protein expressions were enhanced with the hippocampus isolated from the Sco group. Nonetheless, pretreatment with MOO only, but not with MOE, ameliorated the enhanced protein expression levels of TrkB and NF-κB (p < 0.05 and p = 0.09, respectively).

Conclusion

Our data reveal that MOO is preferable to MOE as a neuroprotective as evidenced by improving memory impairment. This effect, at least in part, through inhibiting the AChE and NF-κB activities and modulating the TrkB expression level.
Prophylactic Effect of Lactobacillus fermentum TKSN02 on Gastric Injury Induced by Hydrochloric Acid/Ethanol in Mice Through Its Antioxidant Capacity
Authors: Hu Tiantian , Zhou Liang , Wang Xiaoli , Zhou Xianrong , Yi Ruokun , Long Xingyao , Zhao Xin
Published: March 2022

In this article, the preventive and protective effect of a new Lactobacillus fermentum, (Lactobacillus fermentum TKSN02: LF-N2), which was isolated and identified from Xinjiang naturally fermented yogurt, on hydrochloric acid (HCl)/ethanol induced gastric injury in mice was studied. A total of 40 mice were divided into the following five groups: normal, model, LF-N2, LB (Lactobacillus bulgaricus), and Ranitidine groups. Except for the normal and model groups, mice in the other groups were treated with LF-N2, LB (Lactobacillus bulgaricus), and Ranitidine separately, and the injury of the gastric tissue was observed by taking photos and pathological sections. The levels of oxidation indicators, gastrointestinal hormone and the inflammatory cytokines in serum and gastric tissue in each group were measured. Further more, the gene expression levels of oxidative stress and inflammation related genes in the colon tissue were determined by the Real-Time PCR method. Pathological observation confirmed that LF-N2 could inhibit the gastric injury caused by HCl/ethanol. Observation of the appearance of the gastric indicated that LF-N2 could effectively reduce the area of gastric injury. Biochemical results showed that the serum gastrin (GAS) and gastric motilin (MTL) levels in the LF-N2 group were significantly lower and the serum somatostatin (SS) level was higher than in the model group and there was no significant difference between all treatment groups. The activities of total superoxide dismutase (T-SOD) and glutathione (GSH) were increased while the malondialdehyde (MDA) content was decreased in LF-N2 treatment group mice, which suggested that LF-N2 has a good antioxidant effect. Further RT-PCR experiments also showed that LF-N2 could promote the related mRNA expression of antioxidant enzymes (Cu/Zn-SOD, Mn-SOD, and CAT) and anti-inflammatory cytokines (IL-4, and IL-10), while it inhibited the gene expression of pro-inflammatory cytokine (IL-6) and apoptosis factor (Caspase-3). As observed, LF-N2 exerted a good preventive effect on HCl/ethanol induced gastric injury in mice, and the effect was close to that of LB, which indicated that LF-N2 has potential use as a probiotic due to its gastric injury treatment effects.
Narrative Review of High-Dose-Rate Interstitial Brachytherapy in Primary or Secondary Liver Tumors
Authors: Karagiannis Efstratios , Strouthos Iosif , Leczynski Agnes , Zamboglou Nikolaos , Ferentinos Konstantinos
Published: March 2022

The optimal management of intrahepatic malignancies involves a multidisciplinary approach. Although surgical resection has been considered the only curative approach, the use of several minimally invasive ablative techniques has dramatically increased the last two decades, mainly due to the fact that they provide similar oncological results with significantly decreased morbidity. Among these modalities, interstitial liver brachytherapy, probably the most flexible liver ablative method, with excellent clinical data on its safety and effectiveness, is frequently not even mentioned as an option in the current peer reviewed literature and guidelines. Brachytherapy is a type of radiotherapy utilizing radionuclides that are directly inserted into the tumor. Compared to external beam radiation therapy, brachytherapy has the potential to deliver an ablative radiation dose over a short period of time, with the advantage of a rapid dose fall-off, that allows for sparing of adjacent healthy tissue. For numerous malignancies such as skin, gynecological, breast, prostate, head and neck, bladder, liver and soft-tissue tumors, brachytherapy as a monotherapy or combined with external beam radiation therapy, has become a standard treatment for many decades. This review article aims to describe the high-dose-rate liver brachytherapy technique, its selection criteria, present its advantages and disadvantages, as well as the available clinical data, in order to help physicians to explore and hopefully introduce liver brachytherapy into their clinical routine.
Retinoic Acid-Induced 2 (RAI2) Is a Novel Antagonist of Wnt/β-Catenin Signaling Pathway and Potential Biomarker of Chemosensitivity in Colorectal Cancer
Authors: Zhang Weitao , Kong Lu , Zhu Hongbin , Sun Decong , Han Quanli , Yan Bin , Cui Zhi , Zhang Weiwei , Zhang Shurong , Kang Xindan , Dai Guanghai , Qian Niansong , Yan Wenji
Published: March 2022

Objective

Aberrant activation of Wnt/β-catenin signaling contributes to the maintenance of cancer stem cells and chemoresistance in colorectal cancer (CRC). Retinoic acid-induced 2 (RAI2) was proved to be a tumor suppressor in CRC in our previous report. In this study, the role of RAI2 in Wnt/β-catenin signaling was further investigated.

Methods

As a transcriptional co-regulator, C-terminal Binding Protein 2 (CtBP2) was reported to be involved in Wnt signaling in multiple and complex ways. The correlation of RAI2 and CtBP2 in CRC was analyzed by TCGA dataset, and the interaction between RAI2 and CtBP2 was explored by co-immunoprecipitation (Co-IP) in CRC cells. The effect of RAI2 on the activity of Wnt signaling and the location of β-catenin was detected by Dual-Luciferase reporter assay and Immunofluorescence respectively. Western blotting analysis was performed to detect the expression of target genes involved in Wnt signaling. Sphere formation assay was employed to detect the effect of RAI2 on stem cell like properties. Cell viability assay was used to detect the chemosensitivity of cells before and after transfection of RAI2.

Results

The interaction between RAI2 and CtBP2 was confirmed by Co-IP in CRC cells. Besides, the negative correlation of RAI2 and CtBP2 in CRC was found by analyzing the TCGA dataset. Re-expression of RAI2 in human colon cancer cells (HCT116 and LoVo) suppressed the fluorescent activity of Wnt signaling, increased the phosphorylation and inhibited nuclear translocation of β-catenin, with down-regulation of target genes like c-Myc, CyclinD1, ASCL2, and LGR5. In contrast, the mutated RAI2, which can’t interact with CtBP2, has no above effects. We observed low expression of RAI2 in 33.89% (101/298) of CRC patients, which was significantly associated with reduced phosphorylation of β-catenin (r=0.8866, P<0.0001), poor 5-year relapse-free survival (RFS) (P = 0.0029) and overall survival (OS) (P = 0.0102). Restoration of RAI2 in HCT116 and LoVo cells inhibited stem cell-like properties of CRC cells and increased chemosensitivity of these cells to oxaliplatin and fluorouracil.

Conclusion

Low expression of RAI2 can serve as an independent poor prognostic marker. RAI2 inhibits Wnt signaling by interacting with or down-regulating CtBP2, resulting in repression of stem cell-like properties and increased chemosensitivity of CRC cells.
A Metastatic Intrahepatic Cholangiocarcinoma With HPCs Features: Report of a Case
Authors: Fu Qiang , Liu Pan , Jin Shangkun , Zhang Xu , Liu Chuanjiang , Hu Mingxing , Wang Yuzhu , Zhang Hongwei , Qin Tao
Published: March 2022

Intrahepatic cholangiocarcinoma (ICC) is a highly lethal hepatobiliary neoplasm, which originates from the bile ducts proximal to the second-order division. ICC can be anatomically divided into two subtypes: the large duct type (mucin-production ICC, muc-ICC) and the small duct type (mixed-ICC) origins from hepatic progenitor cells (HPCs). The immunoreactivity of S100P and neural cell adhesion molecule (NCAM) are useful biomarkers to distinguish the two subtypes. In this study, we report a difficult-to-diagnose case of metastatic retroperitoneal tumor of occult hepatolithiasis-associated ICC. Besides, this case was both positive for S100P and NCAM, considered as a rare muc-ICC with the HPCs features. Tumor whole exome sequencing detection results by Genetron (China) revealed that there were 41 gene mutations in this patient. The SMAD4-p.His530ThrfsTer47 and KRAS-p.Gly12Val mutation might promote the occurrence and distant metastasis of the tumor.
Effect of Supervised Resistance Training on Arm Volume, Quality of Life and Physical Perfomance Among Women at High Risk for Breast Cancer-Related Lymphedema: A Study Protocol for a Randomized Controlled Trial (STRONG-B)
Authors: Ramírez-Parada Karol , Lopez-Garzon Maria , Sanchez-Rojel Cesar , Petric-Guajardo Militza , Alfaro-Barra Margarita , Fernández-Verdejo Rodrigo , Reyes-Ponce Alvaro , Merino-Pereira Gina , Cantarero-Villanueva Irene
Published: March 2022

Objectives

To determine the preventive effects of supervised resistance training on arms volume, quality of life, physical performance, and handgrip strength in Chilean women at high risk for breast cancer-related lymphedema (BCRL) undergoing chemotherapy.

Design

Randomized control trial.

Participants

One hundred and six women at high risk for breast cancer-related lymphedema aged 18 to 70 years.

Interventions

Participants will be randomized into two groups: [a] intervention, who will receive 12 weeks of supervised resistance training (STRONG-B) during adjuvant chemotherapy; and [b] control, who will receive education to promote lymphatic and venous return, maintain range of motion, and promote physical activity.

Main Outcome Measures

The primary outcome will be arms volume measured with an optoelectric device (perometer NT1000). Secondary outcomes will be quality of life, handgrip strength, and physical performance. Primary and secondary outcomes will be measured at baseline, just after the intervention, and 3 and 6 months after. Statistical analysis will be performed following intention-to-treat and per-protocol approaches. The treatment effect will be calculated using linear mixed models.

Discussion

The STRONG-B will be a tailored supervised resistance training that attempts to prevent or mitigate BCRL in a population that, due to both intrinsic and extrinsic factors, will commonly suffer from BCRL.

Clinical Trial Registration

[https://clinicaltrials.gov/ct2/show/NCT04821609], identifier NCT04821609.
Effects of the Anti-Tumor Agents Trabectedin and Lurbinectedin on Immune Cells of the Tumor Microenvironment
Authors: Allavena Paola , Belgiovine Cristina , Digifico Elisabeth , Frapolli Roberta , D’Incalci Maurizio
Published: March 2022

Immune cells in the tumor micro-environment (TME) establish a complex relationship with cancer cells and may strongly influence disease progression and response to therapy. It is well established that myeloid cells infiltrating tumor tissues favor cancer progression. Tumor-Associated Macrophages (TAMs) are abundantly present at the TME and actively promote cancer cell proliferation and distant spreading, as well as contribute to an immune-suppressive milieu. Active research of the last decade has provided novel therapeutic approaches aimed at depleting TAMs and/or at reprogramming their functional activities. We reported some years ago that the registered anti-tumor agent trabectedin and its analogue lurbinectedin have numerous mechanisms of action that also involve direct effects on immune cells, opening up new interesting points of view. Trabectedin and lurbinectedin share the unique feature of being able to simultaneously kill cancer cells and to affect several features of the TME, most notably by inducing the rapid and selective apoptosis of monocytes and macrophages, and by inhibiting the transcription of several inflammatory mediators. Furthermore, depletion of TAMs alleviates the immunosuppressive milieu and rescues T cell functional activities, thus enhancing the anti-tumor response to immunotherapy with checkpoint inhibitors. In view of the growing interest in tumor-infiltrating immune cells, the availability of antineoplastic compounds showing immunomodulatory effects on innate and adaptive immunity deserves particular attention in the oncology field.