bluuebunny/crossref_metadata_2025 huggingface dataset:
Utility of UroVysion Fluorescence in situ Hybridization in Improving the Diagnostic Performance of Urine Cytology
Authors: Sharan K.C. , Srinivasan Radhika , Uppal Radha , Rohilla Manish , Dey Pranab , Kakkar Nandita , Mavuduru Ravimohan S.
Published: July 2024

Introduction: The atypical urothelial cell (AUC) category in The Paris System (TPS) in urine cytology (UrCy) is a challenging area. This study aimed to evaluate the UroVysion fluorescence in situ hybridization (U-FISH) assay in predicting the outcome of AUC. Additionally, we explored the association of abnormal U-FISH results in high-grade urothelial carcinoma (HGUC) concerning muscularis propria invasion (MPI). Methods: This is a retrospective study, and U-FISH was done on archived Papanicolaou-stained smears. Four cohorts were included: non-neoplastic AUC (AUC-NN), neoplastic AUC (AUC-N), muscle-invasive HGUC (HGUC-MI), and muscle-free HGUC (HGUC-MF) outcome on histopathology (HPE) and with clinical follow-up of 12–29 months. U-FISH was evaluated for diagnostic purposes, and MPI and tumor stage prediction by urine FISH score (UFS; high vs. low) based on copy number gain of chromosomes (Chr). Results: U-FISH was performed on 70 cases (20 AUC-NN, 20 AUC-N, 15 HGUC-MI, and 15 HGUC-MF) and was successful in 58/70 (82.85%) cases. All UC cases showed polysomy of ≥2Chr, and all the AUC-NN cases reported non-neoplastic on HPE were negative for U-FISH. U-FISH picked up all carcinoma cases in the AUC-N cohort. Chr 3 polysomy was statistically significant in differentiating HGUC-MI from HGUC-MF and low-grade urothelial carcinoma cases. Chr 3 signals with a cut-off of 6 signals could identify MPI with a sensitivity of 80.95% and specificity of 41.94%. The UFS of the HGUC-MI group was significantly higher than HGUC-MF. Conclusions: U-FISH successfully identified all cases of AUC with neoplastic outcomes. In the HGUC group, there was a difference in cases with and without MPI, which requires further confirmation in a larger prospective cohort.
Clinical Characteristics and Outcomes of Hyperphosphatemia in patients with CKD Stages 1 to 2
Authors: Xie Chao , Gao Qi , Liu Jiao , Su Licong , Pang Mingzhen , Zhou Shiyu , Kong Yaozhong , Nie Sheng , Liang Min ,   the CRDS study Investigators
Published: July 2024

Introduction: There was limited research on the epidemiology of hyperphosphatemia in early-stage chronic kidney disease (CKD) patients. We aimed to explore the clinical characteristics and prognostic value of hyperphosphatemia in patients with CKD stages 1-2.
Methods: We enrolled adult patients with CKD stages 1-2 from 24 regional central hospitals across China. Hyperphosphatemia was defined as a serum phosphate level exceeding 1.45 mmol/L. The study outcomes included all-cause and cardiovascular (CV) mortality. Cox proportional hazard models were used to investigate the association of hyperphosphatemia with all-cause and CV mortality.
Results: Among 99,266 patients with CKD stages 1-2 across China, the prevalence of hyperphosphatemia was 8.3%. The prevalence of hyperphosphatemia was increased with the level of urinary protein and was higher in younger and female patients. Among 63,121 patients with survival information, during a median of 5.2 years follow-up period, there were 436 (8.0%) and 4,695 (8.1%) deaths in those with and without hyperphosphatemia, respectively. After adjusting for potential confounders, compared with patients without hyperphosphatemia, patients with hyperphosphatemia was associated with a higher risk of all-cause mortality (HR, 1.28, 95% CI, 1.16-1.41). Although nearly 60.3% of hyperphosphatemia could be relieved without phosphate-lowering drug therapy among patients with CKD stages 1-2, transient hyperphosphatemia was also associated with an increased risk of all-cause mortality (P=0.048).
Conclusions: Hyperphosphatemia was not rare in patients with CKD stages 1-2 and was associated with an increased risk of mortality. Clinicians should closely monitor serum phosphorus levels in patients with CKD, even in those with normal kidney function.
A Novel Loss-of-Function Variant in COL4A3 in a Consanguineous Moroccan Family Displaying the Alport Syndrome with Variable Clinical Expression
Authors: Taroua Oumayma , Askander Omar , Rhou Hakima , Bouhouche Ahmed
Published: July 2024

Introduction: Alport syndrome (AS) is a rare genetic disorder characterized by abnormalities in the kidneys, ears, and eyes. Its clinical presentation typically manifests in childhood or adolescence and varies widely among affected individuals, ranging from isolated hematuria to end-stage renal disease. The genetic causes of AS primarily involve mutations in the genes encoding type IV collagen COL4A3, COL4A4, and COL4A5, which play essential roles in maintaining the structural integrity of the glomerular basement membrane in the kidney, the cochlea, and the retina. They can be transmitted in autosomal dominant, autosomal recessive, and X-linked recessive. Case Presentation: Here we report a Moroccan consanguineous family with an 18-year-old girl who presented with advanced renal failure and microscopic hematuria. Her audiometry revealed hearing impairment. Urinalysis performed in all the asymptomatic family members showed microscopic hematuria in the mother and younger sister, while computed tomography excluded a urologic cause. Using next-generation sequencing analysis, we identified in the proband a nonsense homozygous variant in the COL4A3 gene (c.4114C>T, p.Gln1372Ter) that was never reported in the literature, and which is considered pathogenic according to the ACMG classification. Segregation analysis in the family showed that the parents were heterozygous like the elder brother, whereas the younger sister was mutated homozygous, and the other brother was homozygous normal. Conclusion: We report a novel nonsense pathogenic variant in COL4A3 that expends the allelic spectrum in AS. Clinical exploration and genetic testing of all the family members revealed intrafamilial clinical variability, suggesting a pseudo-dominant inheritance and a reduced penetrance.
Contemporary Incidence of Cognitive Impairment or Dementia in Patients Undergoing Coronary Artery Bypass Grafting: A Systematic Review and Meta-Analysis
Authors: Lo Hui Zhen , Wee Caitlin Fern , Low Chen Ee , Teo Yao Hao , Teo Yao Neng , Yun Choi Ying , Syn Nicholas L. , Tan Benjamin Y.Q. , Chai Ping , Yeo Leonard L.L. , Yeo Tiong-Cheng , Chong Yao Feng , Poh Kian-Keong , Kong William K.F. , Wong Raymond C.C. , Chan Mark Y. , Sia Ching-Hui
Published: July 2024

Introduction: Despite the high prevalence of cognitive impairment or dementia post-coronary artery bypass grafting (CABG), the incidence of cognitive impairment or dementia post-CABG in contemporary practice is currently unclear. Therefore, this paper aims to investigate the incidence and associated risk factors of cognitive impairment or dementia in patients’ post-CABG. Methods: A systematic search across three databases (PubMed, SCOPUS, and Embase) was conducted for studies published in or after 2013 that reported cognitive impairment or dementia post-CABG. Subgroup analyses and meta-regression by risk factors were performed to determine their influence on the results. Results: This analysis included 23 studies with a total of 2,620 patients. The incidence of cognitive impairment or dementia less than 1 month, 2 to 6 months, and more than 12 months post-CABG was 35.96% (95% confidence interval [CI]: 28.22–44.51, I2 = 87%), 21.33% (95% CI: 13.44–32.15, I2 = 88%), and 39.13% (95% CI: 21.72–58.84, I2 = 84%), respectively. Meta-regression revealed that studies with more than 80% of the cohort diagnosed with hypertension were significantly associated with incidence of cognitive impairment or dementia less than 1 month post-CABG. Conclusion: This meta-analysis demonstrates a high incidence of cognitive impairment or dementia in patients’ post-CABG in contemporary practice, particularly less than 1 month post-CABG and more than 12 months post-CABG. We found that hypertension was a significant risk factor in the short-term (less than 1 month) follow-up period for cognitive impairment or dementia post-CABG. Future research should be done to assess strategies to reduce cognitive impairment post-CABG.
Reproducibility of the Paris System in Urine Cytology by Third-Year Pathology Residents
Authors: Kavas Gamze , Demiröz Asena , Polat Unal Sinem Eser , Calışkan Ahmet Cahit , Ilhan Gözde , Celik Betül
Published: July 2024

Introduction: Standardized basic morphology and the algorithmic approach make the Paris System (TPS) for Reporting Urinary Cytology understandable and applicable. This study examined how well the TPS categories are understood by pathology residents and how well these criteria are enabling them reaching accurate diagnosis. Materials/Methods: A hundred consecutive cases representing all categories were selected. Authors reevaluated slides using TPS regardless of their original diagnosis. In the next step, the TPS was explained to four residents and trained them by five optimal urine cytology samples from each category. Then they were asked to diagnose the selected slides according to the TPS. The diagnoses were compared to authors. The agreement was assessed using kappa. Discordant diagnoses were classified as high and low impact based on potential on clinical practice. Results: The sensitivity of authors was 62.8%, and residents’ were 24–31.8%. The specificity of authors was 98.8%, and residents’ were 82.3–92.8%. Reproducibility of TPS was 40–46%. Kappa values were below 0.40 except for one resident. The highest rate of concordance was for negative for high-grade urothelial carcinoma (NHGUC): authors assigned 38 NHGUC (35 biopsy-proven benign cases). Twenty to twenty-six of them were assigned as NHGUC by residents. While authors assigned 42 cases as suspicious for high-grade urothelial carcinoma (SHGUC) or high-grade urothelial carcinoma (HGUC) (35 biopsy-proven malignant cases), residents assigned 22–29 of them. Discordant diagnosis with high clinical implication was 56–63%. Conclusion: Diagnostic accuracy rates of junior pathology residents using the TPS were unsatisfactory. The best agreement was observed in NHGUC and HGUC categories. Combining HGUC and SHGUC doubled the sensitivity of residents.
Disaster Mitigation Using Disaster Education in High Schools in Banyuwangi and Lumajang Regency
Authors: Retna Ngesti Sedyati  , Suharso Pudjo , Tiara
Published: July 2024

This article describes the urgency of a disaster education curriculum based on local wisdom as a result of a research study in Banyuwangi and Lumajang Regencies, East Java. These two areas are areas with high disaster risk. Banyuwangi Regency is faced with the risk of a tsunami and Mount Raung eruption, while Lumajang is faced with a flood and Mount Semeru eruption. The research was conducted through surveys, literature studies and qualitative descriptions. Methods of data collection through questionnaires, observation, and literature review. Data analysis uses simple inferential statistics and the Miles Hubberman model. As a result, several high schools in Banyuwangi and Lumajang regencies already have a disaster curriculum. The existing disaster curriculum accommodates local wisdom, as an effort to provide appropriate education to face and manage disasters simultaneously. The developed local wisdom-based curriculum explains the relationship between humans and the natural and cultural environment that surrounds them. The implementation of disaster education is carried out through the integration of disaster education in related subjects and through extracurricular activities. The content of disaster education is a combination of disaster standard materials and local wisdom.
The Impact of Hegu Point (LI4) Acupressure Therapy and Warm Compress on Menstrual Pain (Dysmenorrhea) in Middle School Students
Authors: Agustini IGA Ratih , Nilawati Ni Kadek Ayu , Purwaningsih Ni Komang
Published: July 2024

Introduction: Menstruation often leads to disturbances in women, with menstrual pain, or dysmenorrhea, being a common issue that affects daily activities. Non-pharmacological therapies like acupressure and warm compresses are considered safe and effective in managing dysmenorrhea. This study examines the effect of Hegu Point (LI4) acupressure therapy combined with warm compresses on menstrual pain in students at SMP Wisata Denpasar.
Methods: This quantitative study employed a pre-experimental, one-group pre-test, and post-test design. The sample size was 52 students, selected through purposive sampling. The intervention involved applying a warm compress and acupressure at the Hegu point (LI4). Pain levels were measured using an observation sheet for pain scales, and the data were analyzed using the Wilcoxon rank test.
Results: After the intervention, menstrual pain significantly decreased. Before the intervention, 57.7% of the students experienced moderate pain. After the intervention, 53.8% reported mild pain. The Wilcoxon test yielded a p-value of 0.000, indicating a significant effect.
Conclusion: In students, Hegu Point (LI4) acupressure therapy combined with a warm compress significantly reduces menstrual pain. Healthcare providers should educate students about these non-pharmacological therapies for managing dysmenorrhea.
Effect of Strategic Intervention Materials on the Learning Competencies of Learners
Authors: Dumdumaya Chona C. , Nahial Wenie L. , Sabacajan Bazil T. , Morados Alexander N.
Published: July 2024

In response to the alarming performance of the learners in the core subjects, this study aimed to improve the learning outcomes using the Strategic Intervention Materials (SIM) in Grade 6 Science subject. Two intact classes with a total of forty-six (46) Grade VI learners were used as participants of the quasi-experimental study. By random approach, the control group had 20 learners, and 26 learners in the experimental group. The effect of SIM to the performance of the struggling learners in science subject was determined using the pre-test and post-test scores of the two groups of participants. Appropriate statistical tools were used to analyse the test scores of the participants by observing the assumptions of parametric test. Results showed that the pre-test scores between the two groups of participants at the start were very comparable. However, the post test scores of the participants from the experimental and control groups were statistically different with a medium effect size. Moreover, the performance of the female participants significantly exceeded the performance of their male counterparts. This was due to the diligence of the female participants in keeping and handling of the SIM for their advantage. The researcher concluded that SIM significantly helped improve the test scores of the learners. For this, it is recommended that SIM be also applied to other learning areas in schools.
Extent of Students Involvement in Extracurricular Activities and Academic Performance Among Grade 8 Students
Authors: . Vispo Genesis B , Macalinao Zenvi Ann
Published: July 2024

This research study explored the extent of extracurricular activities on student’s academic performance. The study aimed to address the students’ involvement in extracurricular activities and academic performance. It was conducted among the Grade 8 High School Students of Zambales National High School. This study utilized a descriptive quantitative research design, a survey questionnaire was used as the main research instrument for obtaining data and analyzing quantitative data gathered from the respondents. Based from the findings, it concludes that the majority of respondents were female students aged 13-14 with a middle-income family income. Sports was the most involved, and students did not participate in debates. The level of involvement in extracurricular activities was slightly involved. The students rated satisfactory academic performance. There was no significant difference in involvement based on profile variables or academic performance. The study recommends that secondary schools should consider extracurricular activities as a way to promote student growth and provide experiences outside the classroom. School counselors should work with students to identify activities that align with their interests and skill levels.
Unveiling the Confidence of Criminology Practitioners: Delving on Research Knowledge and Attitude
Authors: Nayoyos-Refugia Jennifer , Dalugdog Wilfredo D. , Dausan Alrien F. , Villa Elizabeth Buena
Published: July 2024

This study aims to investigate the level of confidence among criminology practitioners in their research knowledge and attitudes towards research. By examining both aspects, the study seeks to understand how these factors influence overall confidence in engaging with and applying research findings. Furthermore, this descriptive study explores whether there is a significant difference between practitioners' confidence levels related to their research knowledge and their attitudes towards research. Understanding these dynamics is crucial for enhancing the integration of research into criminology practice, thereby improving the effectiveness of interventions and policies. The study utilized a descriptive design having 128 qualified respondents using purposive sampling that answered the self-made questionnaire. Result shows that among the practitioner, academicians have higher level of confidence in terms of research knowledge and attitude. There is a significant difference between the level of confidence relative to research knowledge and attitude. Likewise, the research knowledge of the criminology practitioners has positive relationship among the scope of practice. Hence, the findings suggest that in order to improve the research culture within the field of criminology, a professional development plan might be created to address and cater to all criminology practitioners within the discipline, with guidelines based on the scope of practice under RA 11131.
Reading Partnership between Challenged and Knowledgeable (Repack) Read-ers to Improve Performance
Authors: Enriquez Rinalyn D.
Published: July 2024

Reading has been capitalized in the education process. It has been put premium in the context of the learning recovery as the return of in-person of classes is observed. This action research aimed to examine the reading performance level of Grade One pupils of Camiing Elementary School before and after the employment of project RePACK (Reading Partnership between Challenged and Knowledgeable). This study further determined the aspects of the introduced intervention that facilitated their reading performance. The participants of the study were the 45 Grade one pupils of Camiing Elementary School School of Schools Division of Zambales in the school year 2022-2023. The data were gathered through a mixed method. The qualitative part employed thematic analysis to interpret the transcribed interviews. On the other hand, the quantitative one-group pretest-post-test design used the mean and paired sample t-test to measure the reading performance of the learners in the phases of treatment. The analysis of how the project RePACK facilitate their reading performance has emerged into two themes: availability of learning support, and access to reading resources. The level of learners’ oral word recognition in the pre-intervention was found frustration and instructional in the post-intervention. Similarly, there was a leaped in progression of the reading comprehension of the leaners where they were marked frustration in the pre-test and independent in the post-test. Notably, there is a significant difference in the learners’ reading performance level before and after the intervention. This outcome suggests that the project RePACK is effective in improving the reading performance of the learners. In this regard, school heads, teachers, and students are encouraged to adopt the intervention because of its efficacy.
Relevance of Coast Guard Public Affairs Service: Basis for Development of Officer’s Course Instructional Guide
Authors: Leanda Jem Marie D
Published: July 2024

With the rapid change of information environment, this study explored the relevance of the Coast Guard Public Affairs Service (CGPAS) in executing the functions of the Philippine Coast Guard (PCG). The research highlights the essential skills of Public Affairs Officers (PAOs) in these areas: Media Relations, Public Information Dissemination, and Social Media Management in promoting a positive image of the PCG.
The study utilized survey and interview to gather data from 40 PAOs, majority with the rank of Ensigns, with at least six months PAO experience; 84 Heads of Offices (HOs), who were mostly Captains; and four Subject Matter Experts (SMEs) in Public Affairs, Education, and Training, all from the PCG. Results revealed that PAOs, HOs and SMEs concurred on the crucial role of CGPAS in the execution of PCG functions, particularly in crisis communication and public image promotion. In terms of skill levels of PAOs, the overall scenario-based exam results coincided with the overall HO’s perception. Both were equivalent to Competent, based on the Dreyfus Model of Skill Acquisition. The study also identified challenges faced by PAOs, including role overload from multiple designations and inadequate resources such as office space, trained spokesperson and PA equipment.
Recommendations included providing better equipment, comprehensive trainings on crisis communication, news writing and cybersecurity, fostering organizational collaboration, and recruiting specialists.  Finally, a Course Instructional Guide for Public Affairs Officers was developed and proposed for adoption and implementation to hone their effectiveness in promoting the PCG’s mission and functions.
Level of Effectiveness and Seriousness on The Problems Encountered in the Implementation of Crime Prevention Strategies of The Philippine Na-tional Police in Olongapo City, Philippines
Authors: Binan Generico M. , Dausan Alrien Francisco , Echaure Jessie Sabellano
Published: July 2024

Every country is impacted by criminality on an economic, social, and political level. The national news in the Philippines typically reports on crimes against persons and property because of the prevailing belief that the country's economic standing is insufficient to support an individual's level of living. The Philippine National Police (PNP) in Olongapo City has not yet succeeded in implementing crime prevention and control, despite their best efforts to prevent crime. The Philippines uses foreign approaches to crime prevention that don't align with the nation's current criminality issues. The PNP organization is facing difficulty in crime prevention due to its inability to demonstrate its efficacy and efficiency in the performance of its duty. The primary aspect that leads to the prevention of crime is following the path of morality, virtue, and ethics by all the individuals. These morals and values would enable a person to focus on the achievement of his life and career goals and abstain from conducting any kind of criminal and violent acts.
Land conflict resolution: Case study in Sarirejo Village
Authors: Aritonang Juniaty D. , Hidayat  , Zuska Fikarwin
Published: July 2024

This study aims to describe the factors that cause land conflicts between the community and the Air Force in the Indonesian Air Force in Suwondo and the strategies that the community uses in demanding their land rights. The author chose a qualitative approach with the ethnographic method to understand more deeply what is behind an event that took the process, causes and conflict resolution. The results showed that the factor causing the conflict was the claim of each party to the land. Conflict resolution efforts are carried out by the community to obtain land rights through non-litigation advocacy processes and litigation advocacy. The results of these two strategies were able to encourage the government to restore community rights to their land even though it had to go through a long struggle. In July 2020 the government issued a policy to move the Sowondo Base to Langkat Regency.
Quantifying LULC Changes in Constantine, Algeria Using Google Earth Engine
Authors: Benoumeldjadj Maya , Rached-Kanouni Malika , Bouchareb Abdelouahab , Ababsa Labed
Published: July 2024

The objective of this research is to analyze land use changes in the municipality of Constantine over a thirty year period (1993 – 2023) using Landsat satellite data provided through the Google Earth Engine (GEE) platform and a supervised classification approach. Five land cover classes were mapped at six key dates: water bodies, green spaces, built-up areas, bare soil and agricultural land. The quantified changes highlight a considerable reduction in bare soil in favor of an increase in cultivated land, as well as an expansion of artificial surfaces during certain periods (2008 to 2023). These dynamics reflect the processes of urban sprawl and peri-urbanization at work in this territory. The results obtained demonstrate the potential of remote sensing through GEE for detailed spatio-temporal monitoring of land use. Coupled with other data, this approach contributes to a better understanding of territorial evolutions and provides valuable information for sustainable land use planning.
Analysis of Public Service Performance at the Regional Revenue Agency (BAPENDA) of Bengkulu City
Authors: Sitanggang Sintiani O. , Hakim Kahar , Nursanty
Published: July 2024

This article presents an analysis of public service performance at the Badan Pendapatan Daerah (Bapenda) of Bengkulu City, focusing on the collection of motor vehicle taxes and vehicle title transfer fees in 2023. In the context of this study, the effectiveness of Bapenda is analyzed across three aspects: production, quality, and efficiency, and is also examined from the dimensions of flexibility and employee satisfaction. The research method employs a descriptive-analytical approach by collecting data through observation, interviews, and analysis of related documents. The analysis results indicate that the effectiveness of Bapenda in managing regional revenue is still not optimal, particularly in terms of achieving revenue targets, service quality, and expenditure management efficiency. Although efforts have been made to verify the accountability of APBD implementation, errors in financial management by organizational units are still found. Based on these findings, it is concluded that Bapenda needs improvements in various aspects to enhance its effectiveness, including better coordination among units, increased understanding of applicable regulations, and improved employee satisfaction in performing verification tasks. The implications of this study highlight the importance of strengthening internal supervision and control in regional financial management to improve overall public service performance.
Development of Low-Cost Technology-Based Learning Aids for Heat Radiation Material
Authors: Dewi Siska , Hakim Lucky Maulana
Published: July 2024

This study aims to develop low-cost technology-based learning aids in physics, specifically on heat radiation, using Student Worksheets (LKS). The Suyanto method of research and instructional media development was employed, including internal testing and variance similarity testing of the learning aids. The results consist of physics learning aids and LKS that have been internally validated, in line with theoretical expectations. The LKS validation yielded a score of 3.14, indicating suitability for use, while the external test using a One-Shot Case Study showed a student learning outcome score of 3.61, signifying good learning quality. Assessment of the appeal of the learning aids and LKS yielded a score of 3.32, and ease of use scored 3.17, indicating positive reception. Improvements were made before the production stage, which only involved prototyping.
Do planisférico ao global: a espacialização da economia-mundo europeia e a Terra dos Patos (c. 1600-1650)
Authors: Oliveira Tiago Kramer de
Published: July 2024

O topônimo Patos aparece em diversos mapas do século XVII para identificar uma área do litoral sul do Brasil. O estudo da bibliografia e da documentação — crônicas, correspondências, documentação administrativa, mapas — permitiu desvendar características de agentes sociais, espaços, práticas econômicas e relações de poder que podem ser analisadas atendendo às atuais demandas por abordagens que promovam pessoas e espaços locais ao centro da análise das dinâmicas globais. Patos foi local de disputas entre sacerdotes, autoridades coloniais, colonos, sertanistas, povos indígenas, comerciantes, corsários e personagens que fizeram mais de um desses papéis. Defende-se a pertinência da unidade de análise economia-mundo, de Fernand Braudel, para analisar a globalidade dessas dinâmicas locais, ao mesmo tempo que se propõe que a dinâmica espacial dessa unidade de análise seja compreendida na complexa interação entre os diversos e conflitantes espaços-tempo que constituem a natureza da economia-mundo europeia na época moderna.
Tecendo a rede de capitais cultural e de ciência em museus de ciência no Rio de Janeiro
Authors: Dahmouche Mônica Santos , Damico José Sergio , Mano Sonia , Cazelli Sibele , Costa Andréa Fernandes
Published: July 2024

Museums are places for potentially promoting emotions and creating memories from the experiences they provide. In this regard, this article investigates why people visit science museums, intending to unveil the public’s diversity and facilitate the understanding of their interests and motivations for this cultural practice. Data were collected by means of a printed and self-applied questionnaire filled out by adult visitors over 30 years old during visits to five science museums located in the metropolitan region of Rio de Janeiro, Brazil. Based on the concepts of cultural capital, science, interest and motivation, the applied analytical approach was qualitative and quantitative aided by the Collective Subject Discourse method. The findings bring contributions to the field of public and cultural leisure studies. Processes of acquiring, expanding and transmitting these forms of capital to children, family, and friends emerge from the discourses during interaction with exhibitions and educational and dissemination activities offered by science museums. This understanding can help to develop museum strategies to attract and retain their spontaneous visiting public.
Museus a céu aberto como agentes de preservação e valorização do patrimônio cultural: uma análise do papel da gestão na transformação da paisagem urbana
Authors: Ribeiro Isabel Cristina Ferreira
Published: July 2024

Este artigo analisa o papel dos museus a céu aberto na preservação e valorização do patrimônio cultural e sua capacidade de transformar a paisagem urbana. Essas instituições são importantes agentes de preservação da cultura e história, promovendo a identidade cultural das cidades. A metodologia envolveu consultas a documentos técnicos, pesquisa bibliográfica e documental. Os museus a céu aberto proporcionam uma experiência única aos visitantes, permitindo o contato direto com as obras em um ambiente natural. A gestão dessas instituições é essencial para garantir a preservação do patrimônio cultural e a proteção da paisagem urbana. Nesse sentido, é importante que as autoridades locais e as comunidades estejam envolvidas na gestão dessas instituições, a fim de promover a preservação e valorização do patrimônio cultural. Com isso, busca-se fornecer compreensão mais ampla do papel dos museus a céu aberto na transformação da paisagem e na promoção da identidade cultural das cidades.
O ensino superior público em Roraima em tempos de mobilidade tecnológica
Authors: Gomes Sandra Maria de Morais , Baptaglin Leila
Published: July 2024

Esta investigação tem por objetivo analisar a Educação Superior Pública de Roraima no século XXI, sob a ótica da mobilidade tecnológica/tecnologia mobile, suas transformações, vulnerabilidades e potencialidades na construção de uma nova Amazônia. Através do método qualitativo, de natureza exploratória, esta investigação faz parte da tese de doutorado em Educação, num formato em rede com nove universidades da Amazônia Legal. Seu locus de pesquisa foi a graduação das três Instituições de Ensino Superior Públicas de Roraima. Os resultados foram obtidos por meio de entrevistas aos pró-reitores de graduação e questionários enviados a professores e alunos. Fica evidente que a mobilidade tecnológica/tecnologia mobile, passa a ser, assim como a Inovação, fundamental para as universidades, num contexto tecnológico, digital e ubíquo.
A práxis e os princípios da educomunicação são decoloniais? Revisando conceitos e aproximações
Authors: Santos Ceres Marisa Silva dos , Teles Edilane Carvalho
Published: July 2024

A Educomunicação tem referências e sustentação no projeto de decolonialidade, por meio da transposição dialógica como medium, que inclui a urgência de uma educação midiática e tecnológica com as realidades e contextos; propõe a (re)construção e o respeito à inclusão das vozes de grupos identitários, secularmente silenciados pela lógica colonialista, como as populações afrodiaspóricas, africanas e indígenas. De metodologia participante e de viés hermenêutico, este artigo analisa sua inserção como uma práxis de intervenção, gênese e imbricamento com os movimentos sociais, culturais e educacionais do campo em emergência.
Mediação como conceito-chave para a Educomunicação: a contribuição da Revista Comunicação e Educação
Authors: Moraes Cláudia Herte de , Gomes Janaina , Casali Caroline
Published: July 2024

This essay reflects on the theoretical bases and methodologies around the concept of mediation to bring knowledge about its approach in issues of Revista Comunicação & Educação, the main publisher of studies in the area in Brazil. It mainly focuses on educommunicative mediation. This exploratory study establishes an intentional sampling to consider the configuration of production by periods, authors, and methodological approaches. It concluded that mediation has persisted over the years as a theme (recently increasing) and that most authors come from southeastern Brazil, especially those affiliated with ECA-USP. This study describes the most cited articles and authors, evaluating the perspectives of cultural mediation and technological mediation as areas of greater visibility.
Comunicação e entretenimento: a mensagem educativa sobre demência na narrativa do game Before I Forget
Authors: Mastrocola Vicente Martin
Published: July 2024

Neste trabalho, tomamos como objeto empírico o game Before I Forget e observamos como um produto de entretenimento pode ser um meio de transmissão para um conteúdo educativo sobre o problema de demência. Por meio de pesquisa bibliográfica e uma observação não-participativa na página do jogo no site Steam – seguindo ideias de Kozinets – buscamos entender como a mensagem do game sobre demência é recebida pelos jogadores ao se colocarem no papel da personagem que sofre da doença em questão. Como principais referenciais teóricos sobre comunicação e educação utilizamos Baccega e Silverstone; em relação à área de jogos e entretenimento, nos valemos de Flanagan e Nissenbaum e Huizinga. Em nossas conclusões, entendemos que jogos como Before I Forget potencialmente apontam estratégias para que um conteúdo da área de saúde tenha enfoque educativo utilizando elementos lúdicos para provocar uma reflexão na audiência.
Espelhamentos: o GPT e a educação
Authors: Citelli Adilson Odair
Published: July 2024

Neste artigo, situamos alguns elementos circundantes do ChatGPT que estão desafiando o campo de estudos educomunicativos. Os avanços em andamento na inteligência artificial generativa vêm provocando alterações nos modos de acessar informações, elaborar discursividades, promover traduções entre variadas línguas, acionar os potenciais intersemióticos, tudo em tempo acelerado e de relativa facilidade operacional. Trata-se, portanto, de um sistema e seus processos econômicos e comunicacionais, com implicações nos diversos segmentos da vida social, cujas potencialidades podem ser identificadas, malgrado pairarem interrogantes acerca do futuro a ele reservado. Neste contexto, consideramos que a educação, em seus diferentes níveis e séries, será instada a implementar estratégias de reconhecimento dos modelos generativos de linguagem, com eles dialogando a partir de uma perspectiva analítico-crítica.
Máquinas de ensinar analógicas: as precursoras da inteligência artificial na aprendizagem
Authors: Leporace Camila
Published: July 2024

This study offers a brief history of the analogue teaching machines created almost a century ago, arguing that they can be considered as the true forerunners of artificial intelligence in education. To do so, it examines the premises of these old machines, pointing out that they closely resemble those currently associated with machine learning systems. These premises include the individualization of teaching, the reduction of the burden of laborious activities on teachers, and a better teaching experience by automating teaching. Current apprehensions about the arrival of AI systems also resemble those that plagued society when analogue machines first appeared - for example, the fear of teachers being replaced by robotic artefacts and the fear of a distancing between students and teachers rather than a closer relationship. By seeking out these connections, this study takes analyses the digital technologies used for learning, trying to help pave the way for a critical reflection on these uses based on valuing the human perspective over artificial intelligence.
Abstract 101: The Smooth Muscle-specific Long Non-coding RNA
Carmn
Is Critical For Maintaining Vascular Smooth Muscle Contractility
Authors: He Xiangqin , Sakamuri Anil , Hu Guoqing , Dong Kunzhe , Ogola Benard O , Brands Michael , Fulton David , Zhou Jiliang
Published: July 2024

Background:
The primary function of vascular smooth muscle cells (SMCs) is to maintain vascular homeostasis through active contraction and relaxation. Recently we uncovered the SMC-specific long non-coding RNA,
Carmn
(Cardiac mesoderm enhancer-associated noncoding RNA), as a critical regulator for maintaining visceral SMC contractile phenotype, but its functional role in vascular SMCs remains unknown.

Methods and Results:
To determine the role of
Carmn
in vascular SMCs, we generated inducible vascular SMC-specific KO (ivKO) mice by crossing floxed
Carmn
mice with the vascular SMC-restricted
Itga8
-CreER
T2
mouse. We found that
Carmn
ivKO mice show normal lifespan, but morphological analysis of the mouse thoracic aorta revealed a thinning vascular wall in ivKO mice compared with control mice. Data from vascular contractility measurements of both carotid and mesenteric arteries showed that vascular contractility in the ivKO mice was impaired compared to control mice. Further, while there was no difference in blood pressure at baseline between ivKO and control mice, as measured by radio telemetry, Angiotensin II-induced elevation of blood pressure was attenuated in ivKO mice. Mechanistically, data from bulk RNA-seq of
Carmn
-deficient mouse aorta revealed that
Carmn
deficiency in vascular SMCs leads to downregulation of genes associated with muscle contraction and regulation of blood circulation while increased expression of genes associated with response to inflammation and extracellular matrix remodeling. Consistently, VSMCs in
Carmn
-deficient aorta displays decreased expression of genes such as
Mylk
and
Dmpk
associated with muscle contraction while increased expression of genes associated with tissue remodeling as revealed by single nuclei RNA-seq.

Conclusions:
Our data suggest that vascular SMC-restricted deletion of
Carmn
attenuates vascular contractility and dampens the response to Angiotensin II-induced hypertension through regulating genes involved in muscle contraction. Our study demonstrates that
Carmn
is critical for maintaining vascular contraction function in mice and suggests
Carmn
may play an important role in SMC-driven vascular diseases in humans.
Abstract 1032: Development Of A Bio-hybrid Endovascular Stent-graft That Enables Immunosuppression Free Islet Cell Transplantation
Authors: Gaston Brandon T , Singh Varun , Kharga Anil , Deng Kevin , Lee Kang Mi , Succi Marc , Dua Anahita , Markmann James F
Published: July 2024

Introduction:
Islet cell transplantation has had limited clinical success freeing type 1 diabetics from exogenous insulin. Currently, islet cell transplants are performed via infusion of islet cells into the recipient's portal vein. Approximately 60% of transplanted cells die within 3 days of transplantation due to ischemic injury. Significant immunosuppression is needed to avoid rejection but is also toxic to islet cells. We have developed a novel endovascular biologic stent-graft that provides cellular immunoisolation while preventing ischemic injury through endovascular deployment (Fig 1).

Hypothesis:
We hypothesize islet cells seeded within the semipermeable biohybrid endovascular graft will survive and secrete insulin in an in-vitro model of perfusion.

Methods:
The semipermeable cell chamber was made from ePTFE with a pore size of 0.22 um, and was seeded with islet cells harvested from BALB/c mice. A normothermic machine perfusion circuit was used to model intravascular deployment. Devices were exposed to varying glucose concentrations and insulin was detected by ELISA.

Results:
There were significantly higher levels of insulin secretion after exposure to high vs low glucose media (p = 0.005). At hour 3 of low glucose (2.8 mM), the average insulin concentration was 76 ± 14 mIU/ml, and at hour 3 of high glucose (28 mM), the average insulin concentration was 134 ± 30 mIU/ml (Fig 2). The rate of insulin secretion was significantly higher in high vs low glucose conditions (6759 ± 503 mIU/h vs. 5344 ± 144 mIU/hr, p = 0.008).

Conclusions:
The endovascular biohybrid device enables islet cell function and survival. In-vivo studies are needed to study the immune interactions.
Abstract 1034: Cardiometabolic Risk Factor Burden Positively Associates With Platelet Immaturity And Size
Authors: Hamo Carine , Rosenfeld Emily , Xia Yuhe , Akinlonu Adedoyin , Luttrell-Williams Elliot , Barrett Tessa , Berger Jeffrey
Published: July 2024

Background:
Cardiometabolic risk factors including, obesity, diabetes and hyperlipidemia contribute to increased cardiovascular disease (CVD). While platelets are involved in the pathogenesis of CVD, the relationship between risk factor burden on platelet indices remains uncertain. Indices of platelet size and immaturity can serve as surrogates for platelet hyperreactivity.

Hypothesis:
We hypothesized that greater cardiometabolic risk factor burden is associated with increased markers of platelet immaturity and platelet size.

Methods:
Blood was collected from CVD-free adults and platelet parameters including platelet count, mean platelet volume (MPV), percent immature platelet fraction (IPF), and absolute immature platelet fraction (AIPF) measured by CBC. Participants were stratified by number of cardiometabolic risk factors (diabetes, obesity (BMI >=30 mg/kg
2
), hyperlipidemia (LDL >=130 mg/dL))We calculated median (IQR) values of platelet indices across categories of risk factor burden. We calculated a p-for-trend across risk factor groups via linear regression.

Results:
Among 141 participants, the mean age was 50.5 ± 14.8 years, 42% were male, 26% Black, 18% Hispanic, 50% had diabetes, 35% obesity and 53% hyperlipidemia. Overall, 14%, 48%, 28%, and 11% had 0, 1, 2 and 3 risk factors, respectively. No association was found between platelet count and risk factor burden. In contrast, risk factor burden was associated with increasing platelet size (p-for-trend 0.0047), percent IPF (p-for-trend 0.0086), and AIPF (p-for-trend 0.0005;
Table
).

Conclusions:
Greater cardiometabolic risk factor burden is associated with increased platelet size and immaturity. These findings offer insight into potential mechanisms linking risk factor burden with CVD.
Abstract 104: Endothelial Restoration Of CAD GWAS Gene PLPP3 By Nanomedicine Suppresses Mechano-sensitive YAP/TAZ Activity And Reduces Atherosclerosis
In Vivo
Authors: Zhu Jiayu , Fang Yun
Published: July 2024

Genome-wide association studies (GWAS) have suggested new molecular mechanisms driving vascular complications such as coronary artery disease (CAD). Nevertheless, a major challenge to developing new therapeutic approaches is to spatiotemporally manipulate these GWAS-identified genes in specific vascular tissues of interest
in vivo
. Atherosclerosis largely occurs in arterial sites where local disturbed flow (DF) activates vascular endothelial cells. The
Yorkie
homologues YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif) have merged as critical transcriptional regulators responding to biomechanical stimuli. The molecular mechanisms by which DF activates while unidirectional flow (UF) inactivates YAP/TAZ remain incompletely understood. Our recent studies demonstrated that DF and genetic predisposition at the CAD locus 1p32.2 cooperatively suppress endothelial phospholipid phosphatase 3 (PLPP3) transcription by inactivating a mechano-sensitive endothelial enhancer; reduced PLPP3 and consequent activation of lipid lysophosphatidic acid (LPA)-receptor signaling, drive endothelial dysfunction. Here we devised a nanomedicine strategy employing VCAM-1-targeting liposome to preferentially deliver chemically-modified
PLPP3
mRNA to inflamed endothelial cells
in vivo
, effectively suppressing endothelial YAP/TAZ activity and reducing DF-induced atherosclerosis in mice. We discovered a molecular paradigm by which
PLPP3
inactivates YAP/TAZ by reducing LPA-induced myosin II and ROCK1. These results highlight a new mechanistic link between GWAS and YAP/TAZ mechano-regulation and moreover, establish the proof of concept of vascular wall-based therapies employing targeted mRNA-based nanomedicine.
Abstract 1044: Single Cell RNA-sequencing Of Endothelial Cells In Atherosclerosis Reveals A Single Phenotypic Transition To A Mesenchymal Cell State
Authors: Dy Danielle , Henson Benjamin D , Lehman Thiel , Shah Jeeya , Wirka Robert
Published: July 2024

Introduction:
Coronary artery disease (CAD) is the leading cause of mortality in the United States. Multiple cell types contribute to the atherosclerotic plaques causing CAD, influencing disease burden. Endothelial-to-mesenchymal transition (EndMT), the process by which endothelial cells (ECs) acquire characteristics of mesenchymal cells and loose canonical endothelial features, is correlated with an unstable plaque phenotype, although this transition remains poorly characterized.

Hypothesis:
A specific population of ECs may undergo EndMT, potentially contributing to the progression of atherosclerosis.

Methods:
Endothelial-specific lineage-tracing was induced in

Tg
Cdh5-CreERT2

,

ApoE
-/-

mice, followed by 16 weeks of high-fat diet (HFD) to produce atherosclerosis. Aortic roots were collected from male and female disease-condition animals (n=13) and a baseline cohort that was not fed HFD (n=6). Single cell RNA sequencing was performed using 10X Genomics and analyzed with the Seurat package. RNAscope staining of sections (n=10 per genotype) was performed to characterize the lesion.

Results:
Multiple endothelial subtypes in the aortic root were identified. One population of arterial ECs, characterized by
Klk10
expression, appears to give rise to a disease-specific population of ECs expressing
Edn1
. These ECs appear to acquire a mesenchymal phenotype, consistent with the occurrence of EndMT. RNAscope staining revealed
Edn1
+ cells at the luminal surface and deep within the plaque. Upstream regulator analysis comparing
Edn1+
and
Klk10+
populations predict EndMT-associated transcription regulators including SNAI2, TWIST2, STAT3, SNAI1 and YAP1 as drivers of this transition. This analysis predicts upregulation of both fibrotic and cytokine signaling. In contrast to a previous study of carotid ECs under disturbed flow that identified multiple disparate EC phenotypes, we observed a single disease-associated EC transition.

Conclusion:
We identified ECs undergoing EndMT in a HFD-induced mouse model of atherosclerosis and determined that this transition is the primary EC cell state transition during disease. Thus, the process of EndMT may account for both mesenchymal and inflammatory changes observed in ECs during atherosclerosis.