bluuebunny/crossref_metadata_2025 huggingface dataset:
Constructing a malaria-related health service readiness index and assessing its association with child malaria mortality: an analysis of the Burkina Faso 2014 SARA data
Authors: Millogo Ourohiré , Doamba Jean E. O. , Sié Ali , Utzinger Jürg , Vounatsou Penelope
Published: January 2021

Background

The Service Availability and Readiness Assessment surveys generate data on the readiness of health facility services. We constructed a readiness index related to malaria services and determined the association between health facility malaria readiness and malaria mortality in children under the age of 5 years in Burkina Faso.

Methods

Data on inpatients visits and malaria-related deaths in under 5-year-old children were extracted from the national Health Management Information System in Burkina Faso. Bayesian geostatistical models with variable selection were fitted to malaria mortality data. The most important facility readiness indicators related to general and malaria-specific services were determined. Multiple correspondence analysis (MCA) was employed to construct a composite facility readiness score based on multiple factorial axes. The analysis was carried out separately for 112 medical centres and 546 peripheral health centres.

Results

Malaria mortality rate in medical centres was 4.8 times higher than that of peripheral health centres (3.5% vs. 0.7%, p < 0.0001). Essential medicines was the domain with the lowest readiness (only 0.1% of medical centres and 0% of peripheral health centres had the whole set of tracer items of essential medicines). Basic equipment readiness was the highest. The composite readiness score explained 30 and 53% of the original set of items for medical centres and peripheral health centres, respectively. Mortality rate ratio (MRR) was by 59% (MRR = 0.41, 95% Bayesian credible interval: 0.19–0.91) lower in the high readiness group of peripheral health centres, compared to the low readiness group. Medical centres readiness was not related to malaria mortality. The geographical distribution of malaria mortality rate indicate that regions with health facilities with high readiness show lower mortality rates.

Conclusion

Performant health services in Burkina Faso are associated with lower malaria mortality rates. Health system readiness should be strengthened in the regions of Sahel, Sud-Ouest and Boucle du Mouhoun. Emphasis should be placed on improving the management of essential medicines and to reducing delays of emergency transportation between the different levels of the health system.
The potential role of local pharmacies to assess asthma control: an Italian cross-sectional study
Authors: Caminati M. , Cegolon L. , Bacchini M. , Segala N. , Dama A. , Bovo C. , Olivieri B. , Furci F. , Senna G.
Published: January 2021

Background

Asthma control and monitoring still represents a challenge worldwide. Although the international guidelines suggest the interplay between secondary and primary care services as an effective strategy to control the disease, community pharmacies’ are seldom involved in asthma control assessment.

The present cross-sectional study aimed at providing a picture of the relationship between asthma severity and control in community pharmacies within the health district of the city of Verona (Veneto Region, North-Eastern Italy).

Methods

A call for participation was launched through the Pharmacists’ Association of Verona. Patients referring to the participating pharmacies with an anti-asthmatic drug medical prescription and an asthma exemption code were asked to complete the Asthma Control Test (ACT) and a brief questionnaire collecting information on their age, sex, smoking status, aerobic physical exercise and usual asthma therapy, which also defined asthma severity. A multinomial logistic regression model was fitted to investigate the risk of uncontrolled as well as poorly controlled vs. controlled asthma (base). Results were expressed as relative risk ratios (RRR) with 95% confidence interval (95%CI).

Results

Fifty-seven community pharmacies accepted to participate and 584 asthmatic patients (54% females; mean-age: 51 ± 19 years) were consecutively recruited from 1st January to 30th June 2018 (6 months). Based upon ACT score 50.5% patients had a controlled asthma, 22.3% a poorly controlled and 27.2% uncontrolled. A variable proportion of patients with uncontrolled asthma were observed for every level of severity, although more frequently with mild persistent form of asthma. Most patients (92%) self-reported regular compliance with therapy. At multinomial regression analysis, patients under regular asthma treatment course (RRR = 0.33; 95%CI: 0.15; 0.77) were less likely to have an ACT< 16 compared to those not taking medications regularly.

Conclusions

Overall, our findings highlighted an unsatisfactory asthma control in the general population, independently of the severity level of the disease. Community pharmacies could be a useful frontline interface between patients and the health care services, supporting an effective asthma management plan, from disease assessment and monitoring treatment compliance to referral of patients to specialist medical consultancies.
Upfront surgery is not advantageous compared to more conservative treatments such as observation or medical treatment for patients with desmoid tumors
Authors: Tsukamoto Shinji , Tanzi Piergiuseppe , Mavrogenis Andreas F. , Akahane Manabu , Kido Akira , Tanaka Yasuhito , Cesari Marilena , Donati Davide Maria , Longhi Alessandra , Errani Costantino
Published: January 2021

Background

This study compared the clinical and functional outcomes of patients initially treated with observation or medical treatment with those of patients treated with local treatment (surgery alone or surgery with adjuvant radiotherapy) to confirm whether observation or medical treatment is an appropriate first-line management approach for patients with desmoid tumors.

Methods

We retrospectively reviewed the medical records of 99 patients with histologically confirmed primary desmoid tumors treated between 1978 and 2018. The median follow-up period was 57 months. We evaluated event-free survival, defined as the time interval from the date of initial diagnosis to the date of specific change in treatment strategy or recurrence or the last follow-up.

Results

An event (specific change in treatment strategy or recurrence) occurred in 28 patients (28.3%). No significant difference in event-free survival was found between the first-line observation/medical treatment and local treatment groups (p= 0.509). The median Musculoskeletal Tumor Society score of the patients treated with first-line local treatment was 29 (interquartile range [IQR], 23–30), whereas that of the patients managed with first-line observation or medical treatment was 21 (IQR, 19–29.5). First-line observation or medical treatment was more frequently chosen for larger tumors (p= 0.045). In the patients treated with local treatment, local recurrence was not related to the surgical margin (p= 0.976).

Conclusion

Upfront surgery is not advantageous compared to more conservative treatments such as observation or medical treatment for patients with desmoid tumors.
Response to ‘Methodological flaws on “manual therapy for the pediatric population: a systematic review” by Prevost et al. (2019)’
Authors: Pohlman Katherine A. , Anderson Kristian , Carleo Beth , Gleberzon Brian
Published: January 2021

Correspondence from Yu et al. identify methodological issues with the systematic review of manual therapy for pediatric manuscript. Like any research study, limitations are important for readers to keep in consideration when reviewing study findings. The primary authors maintain full confidence in the use of the review to provide practicing clinicians with a comprehensive overview of the limited and low-quality available evidence regarding manual therapies for the pediatric patient.
Germacrone alleviates neurological deficits following traumatic brain injury by modulating neuroinflammation and oxidative stress
Authors: Zhuang Sujing , Liu Baogui , Guo Shifeng , Xue Yanzhong , Wu Lin , Liu Shiqi , Zhang Chunling , Ni Xiuyan
Published: January 2021

Background

Germacrone (GM) is a terpenoid compound which is reported to have anti-inflammatory and anti-oxidative effects. However, its role in treating traumatic brain injury (TBI) remains largely unknown.

Methods

Male C57BL/6 mice were divided into the following groups: control group, TBI group [controlled cortical impact (CCI) model], CCI + 5 mg/kg GM group, CCI + 10 mg/kg GM group and CCI + 20 mg/kg GM group. GM was administered via intraperitoneal injection. The neurological functions (including motor coordination, spatial learning and memory abilities) and brain edema were measured. Nissl staining was used to detect the neuronal apoptosis. Colorimetric assays and enzyme linked immunosorbent assay (ELISA) kits were used to determine the expression levels of oxidative stress markers including myeloperoxidase (MPO), malondialdehyde (MDA) and superoxide dismutase (SOD), as well as the expressions of inflammatory markers, including tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6). Additionally, protein levels of Nrf2 and p-p65 were detected by Western blot assay.

Results

GM significantly ameliorated motor dysfunction, spatial learning and memory deficits of the mice induced by TBI and it also reduced neuronal apoptosis and microglial activation in a dose-dependent manner. Besides, GM treatment reduced neuroinflammation and oxidative stress compared to those in the CCI group in a dose-dependent manner. Furthermore, GM up-regulated the expression of antioxidant protein Nrf2 and inhibited the expression of inflammatory response protein p-p65.

Conclusions

GM is a promising drug to improve the functional recovery after TBI via repressing neuroinflammation and oxidative stress.
Siglec-E retards atherosclerosis by inhibiting CD36-mediated foam cell formation
Authors: Hsu Yaw-Wen , Hsu Fu-Fei , Chiang Ming-Tsai , Tsai Dong-Lin , Li Fu-An , Angata Takashi , Crocker Paul R. , Chau Lee-Young
Published: January 2021

Background

The accumulation of lipid-laden macrophages, foam cells, within sub-endothelial intima is a key feature of early atherosclerosis. Siglec-E, a mouse orthologue of human Siglec-9, is a sialic acid binding lectin predominantly expressed on the surface of myeloid cells to transduce inhibitory signal via recruitment of SH2-domain containing protein tyrosine phosphatase SHP-1/2 upon binding to its sialoglycan ligands. Whether Siglec-E expression on macrophages impacts foam cell formation and atherosclerosis remains to be established.

Methods

ApoE-deficient (apoE−/−) and apoE/Siglec-E-double deficient (apoE−/−/Siglec-E−/−) mice were placed on high fat diet for 3 months and their lipid profiles and severities of atherosclerosis were assessed. Modified low-density lipoprotein (LDL) uptake and foam cell formation in wild type (WT) and Siglec-E−/−- peritoneal macrophages were examined in vitro. Potential Siglec-E-interacting proteins were identified by proximity labeling in conjunction with proteomic analysis and confirmed by coimmunoprecipitation experiment. Impacts of Siglec-E expression and cell surface sialic acid status on oxidized LDL uptake and signaling involved were examined by biochemical assays.

Results

Here we show that genetic deletion of Siglec-E accelerated atherosclerosis without affecting lipid profile in apoE−/−mice. Siglec-E deficiency promotes foam cell formation by enhancing acetylated and oxidized LDL uptake without affecting cholesterol efflux in macrophages in vitro. By performing proximity labeling and proteomic analysis, we identified scavenger receptor CD36 as a cell surface protein interacting with Siglec-E. Further experiments performed in HEK293T cells transiently overexpressing Siglec-E and CD36 and peritoneal macrophages demonstrated that depletion of cell surface sialic acids by treatment with sialyltransferase inhibitor or sialidase did not affect interaction between Siglec-E and CD36 but retarded Siglec-E-mediated inhibition on oxidized LDL uptake. Subsequent experiments revealed that oxidized LDL induced transient Siglec-E tyrosine phosphorylation and recruitment of SHP-1 phosphatase in macrophages. VAV, a downstream effector implicated in CD36-mediated oxidized LDL uptake, was shown to interact with SHP-1 following oxidized LDL treatment. Moreover, oxidized LDL-induced VAV phosphorylation was substantially lower in WT macrophages comparing to Siglec-E−/−counterparts.

Conclusions

These data support the protective role of Siglec-E in atherosclerosis. Mechanistically, Siglec-E interacts with CD36 to suppress downstream VAV signaling involved in modified LDL uptake.
HCG11 up-regulation induced by ELK4 suppressed proliferation in vestibular schwannoma by targeting miR-620/ELK4
Authors: Long Ruiqing , Liu Zhuohui , Li Jinghui , Zhang Yuan , Yu Hualin
Published: January 2021

Background

Vestibular schwannoma (VS) is a kind of benign tumor deriving from the acoustic nerve sheath. Substantial long non-coding RNAs (lncRNAs) were illustrated to have crucial roles in multiple cancers. However, few lncRNAs were elucidated in VS.

Methods

HCG11, miR-620 and ELK4 expression were tested by RT-qPCR. Gain-of-function experiments were conducted to confirm the effect of HCG11 on VS.

Results

HCG11 possessed a low expression in VS cell lines. Overexpression of HCG11 repressed cell proliferation but accelerated apoptosis of VS cells. Moreover, we identified ELK4 stimulated the transcription of HCG11 and their affinity was verified by ChIP assays. MiR-620 was chosen to be a target of HCG11 and it was tested to have a high expression in VS cell lines. Moreover, depletion of miR-620 could inhibit cell proliferative ability while fostering apoptosis rate of VS cells. ELK4 was low expressed in VS cell lines and knockdown of ELK4 could rescue the effects made by HCG11 overexpression on progression of VS.

Conclusions

HCG11 could inhibit the growth of VS by targeting miR-620/ELK4 in VS cells. HCG11 was a novel therapeutic target for VS treatment.
Microglial activation contributes to cognitive impairments in rotenone-induced mouse Parkinson’s disease model
Authors: Zhang Dongdong , Li Sheng , Hou Liyan , Jing Lu , Ruan Zhengzheng , Peng Bingjie , Zhang Xiaomeng , Hong Jau-Shyong , Zhao Jie , Wang Qingshan
Published: January 2021

Background

Cognitive decline occurs frequently in Parkinson’s disease (PD), which greatly decreases the quality of life of patients. However, the mechanisms remain to be investigated. Neuroinflammation mediated by overactivated microglia is a common pathological feature in multiple neurological disorders, including PD. This study is designed to explore the role of microglia in cognitive deficits by using a rotenone-induced mouse PD model.

Methods

To evaluate the role of microglia in rotenone-induced cognitive deficits, PLX3397, an inhibitor of colony-stimulating factor 1 receptor, and minocycline, a widely used antibiotic, were used to deplete or inactivate microglia, respectively. Cognitive performance of mice among groups was detected by Morris water maze, objective recognition, and passive avoidance tests. Neurodegeneration, synaptic loss, α-synuclein phosphorylation, glial activation, and apoptosis were determined by immunohistochemistry and Western blot or immunofluorescence staining. The gene expression of inflammatory factors and lipid peroxidation were further explored by using RT-PCR and ELISA kits, respectively.

Results

Rotenone dose-dependently induced cognitive deficits in mice by showing decreased performance of rotenone-treated mice in the novel objective recognition, passive avoidance, and Morris water maze compared with that of vehicle controls. Rotenone-induced cognitive decline was associated with neurodegeneration, synaptic loss, and Ser129-phosphorylation of α-synuclein and microglial activation in the hippocampal and cortical regions of mice. A time course experiment revealed that rotenone-induced microglial activation preceded neurodegeneration. Interestingly, microglial depletion by PLX3397 or inactivation by minocycline significantly reduced neuronal damage and α-synuclein pathology as well as improved cognitive performance in rotenone-injected mice. Mechanistically, PLX3397 and minocycline attenuated rotenone-induced astroglial activation and production of cytotoxic factors in mice. Reduced lipid peroxidation was also observed in mice treated with combined PLX3397 or minocycline and rotenonee compared with rotenone alone group. Finally, microglial depletion or inactivation was found to mitigate rotenone-induced neuronal apoptosis.

Conclusions

Taken together, our findings suggested that microglial activation contributes to cognitive impairments in a rotenone-induced mouse PD model via neuroinflammation, oxidative stress, and apoptosis, providing novel insight into the immunopathogensis of cognitive deficits in PD.
Microglial deletion and inhibition alleviate behavior of post-traumatic stress disorder in mice
Authors: Li Shuoshuo , Liao Yajin , Dong Yuan , Li Xiaoheng , Li Jun , Cheng Yong , Cheng Jinbo , Yuan Zengqiang
Published: January 2021

Background

Alteration of immune status in the central nervous system (CNS) has been implicated in the development of post-traumatic stress disorder (PTSD). However, the nature of overall changes in brain immunocyte landscape in PTSD condition remains unclear.

Methods

We constructed a mouse PTSD model by electric foot-shocks followed by contextual reminders and verified the PTSD-related symptoms by behavior test (including contextual freezing test, open-field test, and elevated plus maze test). We examined the immunocyte panorama in the brains of the naïve or PTSD mice by using single-cell mass cytometry. Microglia number and morphological changes in the hippocampus, prefrontal cortex, and amygdala were analyzed by histopathological methods. The gene expression changes of those microglia were detected by quantitative real-time PCR. Genetic/pharmacological depletion of microglia or minocycline treatment before foot-shocks exposure was performed to study the role of microglia in PTSD development and progress.

Results

We found microglia are the major brain immune cells that respond to PTSD. The number of microglia and ratio of microglia to immunocytes was significantly increased on the fifth day of foot-shock exposure. Furthermore, morphological analysis and gene expression profiling revealed temporal patterns of microglial activation in the hippocampus of the PTSD brains. Importantly, we found that genetic/pharmacological depletion of microglia or minocycline treatment before foot-shock exposure alleviated PTSD-associated anxiety and contextual fear.

Conclusion

Our results demonstrated a critical role for microglial activation in PTSD development and a potential therapeutic strategy for the clinical treatment of PTSD in the form of microglial inhibition.
Statistical analysis plan for the Prophylactic Melatonin for Delirium in Intensive Care (ProMEDIC): a randomised controlled trial
Authors: Wibrow Bradley , Martinez F. Eduardo , Ford Andrew , Kelty Erin , Murray Kevin , Ho Kwok M. , Litton Edward , Myers Erina , Anstey Matthew
Published: January 2021

Rationale

Delirium is defined as acute organic brain dysfunction characterised by inattention and disturbance of cognition. It is common in the intensive care unit and is associated with poorer outcomes. Good quality sleep is important in the prevention and management of delirium. Melatonin is a natural hormone secreted by the pineal gland which helps in the regulation of the sleep-wake cycle. It is possible that melatonin supplementation in intensive care improves sleep and prevents delirium.

Methods and design

The ‘Prophylactic Melatonin for Delirium in Intensive Care’ study is a multi-centre, randomised, double-blinded, placebo-controlled trial. The primary objective of this study is to determine whether melatonin given prophylactically decreases delirium in critically ill patients. A total of 850 ICU patients have been randomised (1:1) to receive either melatonin or a placebo. Participants were monitored twice daily for symptoms of delirium.

Results

This paper and the attached additional files describe the statistical analysis plan (SAP) for the trial. The SAP has been developed and submitted for publication before the database has been locked and before the treatment allocation has been unblinded. The SAP contains details of analyses to be undertaken, which will be reported in the primary and secondary publications.

Discussion

The SAP details the analyses that will be done to avoid bias coming from knowledge of the results in advance. This trial will determine whether prophylactic melatonin administered to intensive care unit patients helps decrease the rate and the severity of delirium.

Trial registration

Australian and New Zealand Clinical Trial Registry (ANZCTR) ACTRN1261600043647, registration date: 06 April 2016. WHO Trial Number – U1111-1175-1814
Association of Aβ deposition and regional synaptic density in early Alzheimer’s disease: a PET imaging study with [11C]UCB-J
Authors: O’Dell Ryan S. , Mecca Adam P. , Chen Ming-Kai , Naganawa Mika , Toyonaga Takuya , Lu Yihuan , Godek Tyler A. , Harris Joanna E. , Bartlett Hugh H. , Banks Emmie R. , Kominek Victoria L. , Zhao Wenzhen , Nabulsi Nabeel B. , Ropchan Jim , Ye Yunpeng , Vander Wyk Brent C. , Huang Yiyun , Arnsten Amy F. T. , Carson Richard E. , van Dyck Christopher H.
Published: January 2021

Background

Attempts to associate amyloid-β (Aβ) pathogenesis with synaptic loss in Alzheimer’s disease (AD) have thus far been limited to small numbers of postmortem studies. Aβ plaque burden is not well-correlated with indices of clinical severity or neurodegeneration—at least in the dementia stage—as deposition of Aβ reaches a ceiling. In this study, we examined in vivo the association between fibrillar Aβ deposition and synaptic density in early AD using positron emission tomography (PET). We hypothesized that global Aβ deposition would be more strongly inversely associated with hippocampal synaptic density in participants with amnestic mild cognitive impairment (aMCI; a stage of continued Aβ accumulation) compared to those with dementia (a stage of relative Aβ plateau).

Methods

We measured SV2A binding ([11C]UCB-J) and Aβ deposition ([11C]PiB) in 14 participants with aMCI due to AD and 24 participants with mild AD dementia. Distribution volume ratios (DVR) with a cerebellar reference region were calculated for both tracers to investigate the association between global Aβ deposition and SV2A binding in hippocampus. Exploratory analyses examined correlations between both global and regional Aβ deposition and SV2A binding across a broad range of brain regions using both ROI- and surface-based approaches.

Results

We observed a significant inverse association between global Aβ deposition and hippocampal SV2A binding in participants with aMCI (r = − 0.55,P = 0.04), but not mild dementia (r = 0.05,P = 0.82; difference statistically significant by Fisherz = − 1.80,P = 0.04). Exploratory analyses across other ROIs and whole brain analyses demonstrated no broad or consistent associations between global Aβ deposition and regional SV2A binding in either diagnostic group. ROI-based analyses of the association between regional Aβ deposition and SV2A binding also revealed no consistent pattern but suggested a “paradoxical” positive association between local Aβ deposition and SV2A binding in the hippocampus.

Conclusions

Our findings lend support to a model in which fibrillar Aβ is still accumulating in the early stages of clinical disease but approaching a relative plateau, a point at which Aβ may uncouple from neurodegenerative processes including synaptic loss. Future research should investigate the relationship between Aβ deposition and synaptic loss in larger cohorts beginning preclinically and followed longitudinally in conjunction with other biomarkers.
English language assessment in Bangladesh today: principles, practices, and problems
Authors: Islam Md Shaiful , Hasan Md Kamrul , Sultana Shahin , Karim Abdul , Rahman Mohammad Mosiur
Published: January 2021

The achievement of curriculum goals and objectives, to a large extent, depends on how assessment methods are designed, implemented, monitored, and evaluated. English language learning in Bangladesh has miserably failed, and ineffective assessment methods may be largely attributed to this failure. This paper attempts to address various aspects and issues of English language assessment in Bangladesh in relation to English language learning as a curricular reform and the education policy of the country. The analysis revealed that there was always a gap between the principles of assessment embedded into the curriculum and the actual assessment practices. Furthermore, heavily hard hit by the high-stakes testing, the curriculum, the learners, and the instructors need to be liberated from this vicious policy. The review concluded with a recommendation that teachers need to develop assessment literacy through teacher education programs that are essential to helping teachers to acquire knowledge, skills, professionalism, and assessment expertise.
Microglial gene signature reveals loss of homeostatic microglia associated with neurodegeneration of Alzheimer’s disease
Authors: Sobue Akira , Komine Okiru , Hara Yuichiro , Endo Fumito , Mizoguchi Hiroyuki , Watanabe Seiji , Murayama Shigeo , Saito Takashi , Saido Takaomi C. , Sahara Naruhiko , Higuchi Makoto , Ogi Tomoo , Yamanaka Koji
Published: January 2021

Microglia-mediated neuroinflammation has been implicated in the pathogenesis of Alzheimer’s disease (AD). Although microglia in aging and neurodegenerative disease model mice show a loss of homeostatic phenotype and activation of disease-associated microglia (DAM), a correlation between those phenotypes and the degree of neuronal cell loss has not been clarified. In this study, we performed RNA sequencing of microglia isolated from three representative neurodegenerative mouse models, AppNL-G-F/NL-G-F with amyloid pathology, rTg4510 with tauopathy, and SOD1G93A with motor neuron disease by magnetic activated cell sorting. In parallel, gene expression patterns of the human precuneus with early Alzheimer’s change (n = 11) and control brain (n = 14) were also analyzed by RNA sequencing. We found that a substantial reduction of homeostatic microglial genes in rTg4510 and SOD1G93A microglia, whereas DAM genes were uniformly upregulated in all mouse models. The reduction of homeostatic microglial genes was correlated with the degree of neuronal cell loss. In human precuneus with early AD pathology, reduced expression of genes related to microglia- and oligodendrocyte-specific markers was observed, although the expression of DAM genes was not upregulated. Our results implicate a loss of homeostatic microglial function in the progression of AD and other neurodegenerative diseases. Moreover, analyses of human precuneus also suggest loss of microglia and oligodendrocyte functions induced by early amyloid pathology in human.
Analytical study of Buddha sculptures in Jingyin temple of Taiyuan, China
Authors: Bai Xiaojian , Jia Chen , Chen Zhigen , Gong Yuxuan , Cheng Huwei , Wang Jiayue
Published: January 2021

With exquisite design and unique style, the painted sculptures of Tutang Buddha and two attendants Buddha in Jingyin Temple are precious cultural heritages of China. The sculpture of Tutang Buddha, which was carved from a mound and painted by ancient craftsmen, was rarely found in ancient China. However, due to natural and human factors, the sculptures were severely damaged. Obviously, they require urgent and appropriate protection and restoration. In this study, samples taken from the sculptures were analysed through multiple analytical techniques, including scanning electron microscopy with energy dispersive spectrometry (SEM–EDS), Raman spectroscopy, X-ray diffraction (XRD), optical microscopy (OM) and granulometry. The analysis results enable us to infer the techniques used by the craftsmen in making the sculptures and provide a reliable evidence for the conservation and future protection of these and similar sculptures.
Reconstructing surface eruptive sequence of 2018 small phreatic eruption of Iwo-yama volcano, Kirishima Volcanic Complex, Japan, by infrasound cross-correlation analysis
Authors: Muramatsu Dan , Matsushima Takeshi , Ichihara Mie
Published: January 2021

The Iwo-yama volcano of the Kirishima Volcanic Complex in Japan had a small phreatic eruption in April 2018, which newly formed multiple vents. The activity was recorded by two infrasound sensors and two monitoring cameras, which had been installed within 1 km of the vents. This study identified infrasonic signals from the multiple vents by a cross-correlation analysis between the two infrasound sensors. The analysis successfully revealed the signals from two main eruption craters and constrained the infrasound onsets at the individual vents in the two craters. We combined the infrasound results with the images from the cameras and reconstructed the sequence of the small phreatic eruption of Iwo-yama. At each of the two craters, the intense eruption, which was depicted by the evident infrasound signals, occurred several hours after the eruption onset. This study provides a sequence of the activities of the multiple vents in a phreatic eruption, which will be useful for understanding the phreatic eruption and hazard assessments.
Classification of topography for ground vulnerability assessment of alluvial plains and mountains of Japan using 30 m DEM
Authors: Iwahashi Junko , Yamazaki Dai , Nakano Takayuki , Endo Ryo
Published: January 2021

This study aims to create a terrain classification of Japan that allows both geomorphological and geoengineering classifications coexist without large contradictions and to distinguish landform elements even in urban plains which include noise associated with digital elevation models (DEMs). Because Japan is susceptible to natural disasters, we designed the classification to reflect the ground vulnerability of both alluvial plains and mountains through the application of terrain classification data to landslide susceptibility and seismic zoning. We updated an existing DEM-based terrain classification method for application in the high-resolution 30 m DEM. We used topographic measurements that do not amplify manmade unevenness or noise, which are usually the main problems associated with the use of high-resolution DEMs with high vertical accuracies. We selected the height above the nearest drainage (HAND), slope gradient, surface texture, and local convexity as geometric signatures, which were devised so as not to detect noise. Segment polygon data of terrain units were derived from the raster data of slope and HAND. The polygon data were classified into 40 clusters using the attributes of slope, HAND, and surface texture; then they were grouped into 16 legends following comparisons with the existing geological and geomorphological maps and supplementary reclassification by HAND and local convexity. The derived terrain classification, except for manmade cuts and fills, showed similarities with the existing expert-driven maps and some association with areas where shallow landslides or floods frequently occur. Based on a trial in California using a 30 m DEM, we concluded that the proposed method can be adopted in other regions outside of Japan.
Nest site selection and reproductive parameters of the threatened Atlantic Royal Flycatcher (Onychorhynchus swainsoni) and their significance for conservation
Authors: Perrella Daniel F. , Zima Paulo V. Q. , Francisco Mercival R.
Published: January 2021

Background

Patterns of rarity can be explained by reproductive rates, levels of endemism, and habitat specificity, and knowledge on these parameters is important to understand the levels of vulnerability of each species and to formulate conservation strategies. Here, we studied nest-site selection and breeding biology of the Atlantic Royal Flycatcher (Onychorhynchus swainsoni), a poorly known vulnerable bird endemic to the Brazilian Atlantic Forest.

Methods

We addressed nest site selection in three different levels: first, we searched for nests near and far from water to investigate whether birds could select water proximities to construct nests; second, we examined if they could select certain streams in detriment of others, and we analyzed the characteristics of used and non-used streams, and third, in streams in which nests were found, we addressed nest site selectivity by comparing a number of parameters between nest sites and random sites. Further, we provide information on breeding biology parameters related to annual fecundity.

Results

During five breeding seasons, we found 23 nests in a well-preserved forest continuum. All of the nests were constructed above water, and they were found in streams that were about 4 m in width, instead of smaller streams with about 1.5 m in width. Modeling analyses revealed that within the used streams, nests were constructed in sites with lower vegetation density in relation to random points, while stream width, water speed, and canopy cover presented no significant correlation. Atlantic Royal Flycatchers in our study had a 22-day incubation period and 24 to 27-day nestling period. Overall nest survival was comparatively high (62%), but clutch size was small (N = 2 eggs) and double-brooding was unlikely, which resulted in a low annual fecundity (1.4 ± 0.9 fledglings per reproductive female). Along the nesting streams, we found an average of 1.62 ± 0.07 breeding pairs/km.

Conclusions

These data suggest that nesting habitat specificity and low annual fecundity are among the factors contributing to the rarity of the Atlantic Royal Flycatcher in large forest continuums and to its absence in fragmented environments. It reinforces the importance of large well-preserved forest continuums for the conservation of habitat specialist Atlantic Forest bird species.
Evaluation of oocyte quality in Polycystic ovary syndrome patients undergoing ART cycles
Authors: Nikbakht Roshan , Mohammadjafari Razieh , Rajabalipour Mina , Moghadam Mahin Taheri
Published: January 2021

Background

To evaluate factors affecting oocyte/embryo quality in PolyCystic Ovary Syndrome (PCOS) patients undergoing Assisted Reproductive Technology (ART) cycles.

Methods

This case-control retrospective study was performed on PCOS patients referred to the infertility department of Imam Khomeini Hospital in Ahvaz from October 2017 to September 2019. Demographic and reproductive characterizations including age, gender, abortion history and infertility type (primary and secondary infertility) were extracted from patient’s records. TSH, AMH, LH, FSH, prolactin, lipid profile and blood glucose was measured. Biochemistry pregnancy was checked by determination of serum βHCG level and then, clinical pregnancy was confirmed by observing of pregnancy sac and fetal heart rate using Transvaginal USS.

Results

One-hundred thirty-five patients include 45 PCOS and 90 Non-PCOS patients with mean age of 31.93 ± 5.04 and 30.8 ± 5.38 (p = 0.24) were considered as case and control groups respectively. Retrieved oocyte numbers were significantly higher in PCOS patients (p = 0.024), but there was no significant difference in number of oocyte subtypes (MI, MII and GV) between two groups. The embryo numbers and its subtypes did not differ significantly in both groups. The clinical pregnancy rate was insignificantly lower in PCOS patients (p = 0.066) and there was a significant correlation between retrieved oocyte numbers with age(r= -0.2, p= 0.022) and AMH level (r = 0.433, p < 0.0001) respectively. Cholesterol level had shown a positive significant correlation with number of MI oocytes (r = 0.421, p = 0.026) and MII oocytes significantly affected by age (r= -0.250, p = 0.004) and AMH level (r = 0.480, p < 0.0001). Using Receiver operation characteristic (ROC) curve analysis, the cut-off value of total number of oocytes was > 10.5 with area under curve of 0.619±0.054(sensitivity 55.56% and specificity 69.66%)

Conclusions

The results of this study showed that although the number of oocytes in PCOS patients was significantly higher than non-PCOS patients, the quality of oocytes was not statistically different. The number and quality of embryos were not significantly different in both groups. Our results indicated a significant relationship between the level of AMH and the number of retrieved oocytes and embryos. We found there is a significant correlation between cholesterol level and number of MI oocytes.
Reasons for discontinuing insulin and factors associated with insulin discontinuation in patients with type 2 diabetes mellitus: a real-world evidence study
Authors: Wu Jianmin , Morrison Fritha , Zhao Zhenxiang , Haynes Ginger , He Xuanyao , Ali Ayad K. , Shubina Maria , Malmasi Shervin , Ge Wendong , Peng Xiaomei , Turchin Alexander
Published: January 2021

Background

Evidence suggests that insulin therapy of patients with type 2 diabetes mellitus (T2DM) is frequently discontinued. However, the reasons for discontinuing insulin and factors associated with insulin discontinuation in this patient population are not well understood.

Methods

We conducted a retrospective cohort study of adults with T2DM prescribed insulin between 2010 and 2017 at Partners HealthCare. Reasons for discontinuing insulin and factors associated with insulin discontinuation were studied using electronic medical records (EMR) data. Natural language processing (NLP) was applied to identify reasons from unstructured clinical notes. Factors associated with insulin discontinuation were extracted from structured EMR data and evaluated using multivariable logistic regression.

Results

Among 7009 study patients, 2957 (42.2%) discontinued insulin within 12 months after study entry. Most patients who discontinued insulin (2121 / 71.7%) had reasons for discontinuation documented. The most common reasons were improving blood glucose control (33.2%), achieved weight loss (18.5%) and initiation of non-insulin diabetes medications (16.7%). In multivariable analysis adjusted for demographics and comorbidities, patients were more likely to discontinue either basal or bolus insulin if they were on a basal-bolus regimen (OR 1.6, 95% CI 1.3 to 1.8; p <  0.001) or were being seen by an endocrinologist (OR 2.6; 95% CI 2.2 to 3.0; p <  0.001).

Conclusions

In this large real-world evidence study conducted in an area with a high penetration of health insurance, insulin discontinuation countenanced by healthcare providers was common. In most cases it was linked to achievement of glycemic control, achieved weight loss and initiation of other diabetes medications. Factors associated with and stated reasons for insulin discontinuation were different from those previously described for non-adherence to insulin therapy, identifying it as a distinct clinical phenomenon.
The time-varying effects of oil prices on oil–gas stock returns of the fragile five countries
Authors: Yurteri Kösedağlı Begüm , Huyugüzel Kışla Gül , Çatık A. Nazif
Published: January 2021

This study analyzes oil price exposure of the oil–gas sector stock returns for the fragile five countries based on a multi-factor asset pricing model using daily data from 29 May 1996 to 27 January 2020. The endogenous structural break test suggests the presence of serious parameter instabilities due to fluctuations in the oil and stock markets over the period under study. Moreover, the time-varying estimates indicate that the oil–gas sectors of these countries are riskier than the overall stock market. The results further suggest that, except for Indonesia, oil prices have a positive impact on the sectoral returns of all markets, whereas the impact of the exchange rates on the oil–gas sector returns varies across time and countries.
Intraguild predation in three generalist predatory mites of the family Phytoseiidae (Acari: Phytoseiidae)
Authors: Momen Faten M. , Abdel-Khalek Amira
Published: January 2021

Background

The predatory mites, Neoseiulus californicus (McGregor), N. barkeri (Hughes), and Amblyseius swirskii Athias-Henriot, are important predators attacking many insect and mite pests. They can coexist in the same habitat and engage in intraguild predation (IGP).

Main body

IGP was assessed among the exotic one N. californicus and the native species N. barkeri and A. swirskii as Intraguild predator (IG-predator)/intraguild prey (IG-prey) in either absence or presence of extra-guild prey Tetranychus urticae Koch (EG-prey). In the laboratory, the physiological parameters, longevity, fecundity, and predation rate of these predatory mites’ females, fed on EG-prey, were evaluated, where phytoseiid larvae are considered as (IG-prey) or combined IG-prey with EG-prey. All predatory species consumed larval stages of each other’s, but in case of N. californicus, females failed to sustain oviposition on N. barkeri larvae. Also, it was noticed that N. californicus females killed 3 times more A. swirskii larvae than N. barkeri larvae, whereas A. swirskii consumed more N. californicus than N. barkeri larvae, respectively. Neoseiulus californicus lived longer on T. urticae and A. swirskii larvae than on N. barkeri, while the latter survived longer on T. urticae only than on the other prey or with combinations with T. urticae. Amblyseius swirskii lived shorter when fed exclusively on T. urticae or IG-prey than on EG-prey combined with IG-prey. In choice experiments, N. californicus showed a higher preference to consume more T. urticae than any of phytoseiid larvae. The comparison between T. urticae and IG-prey diets definite the higher influence of T. urticae on the fecundity in N. californicus and N. barkeri than on IG-prey, whereas in A. swirskii fecundity was as equal on T. urticae as on IG-prey N. californicus larvae.

Conclusion

A. swirskii seemed to be the strongest IG-predator.
COVID-19 lockdown may increase cardiovascular disease risk factors
Authors: Muhammad Daha Garba , Abubakar Ibrahim Ahmad
Published: January 2021

Background

Coronavirus disease 2019 (COVID-19) is a disease caused by severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) and was declared a worldwide pandemic by the World Health Organization (WHO) on 11 March 2020 which is leading to significant morbidity and mortality. In compliance with WHO recommendation of movement restrictions, many countries have imposed compulsory self-quarantine and restricted movements of their citizenries (lockdown/sit at home) and closure of businesses and borders as preventive measures to the fast-spreading virus. Consequently, this decision has made the emergence of behaviors that are detrimental to cardiovascular diseases (CVDs) which are the leading cause of the global mortality rate.

Main body

The increase in sedentary lifestyles, alcohol consumption, and substance abuse during COVID-19 pandemic lockdown as a result of personal restrictions in COVID-19 lockdown is linked with the risk of death from chronic diseases such as cardiovascular diseases (CVDs).

Conclusion

The lockdown has increased risk factors of CVDs, and as such, there might be an increase in the number of non-communicable disease (NCD)-related mortality rate. The effect does not end during the period of coronavirus pandemic but even after the pandemic.
Study of sensory processing deficits in autism spectrum disorder symptom triad: an Egyptian sample
Authors: Reda Mona , Meguid Nagwa A. , Eid Ola M. , Hussein Fatma , Elalfy Dina Y.
Published: January 2021

Background

Autism spectrum disorder (ASD) is one of the most common neurodevelopmental disorders. In DSM-IV, the diagnostic criteria of autism consisted of three domains: impairment in social interaction, communication deficits, and stereotypic behavior, while in DSM-5 they were condensed into two domains: social communication deficits and restricted patterns of behavior to which sensory processing deficits (SPD) were added, manifested by hypo- or hyper-reactivity to sensory stimuli or uncommon interests in sensory aspects of the surrounding environment. The purpose of this study is to determine the relation between SPD and the symptom triad in ASD namely social interaction, communication deficits, and stereotypic behavior. To our knowledge, this issue was not studied before in Egyptian literature.

Results

There was a significant negative correlation between SPD (assessed by short sensory profile: SSP) and symptom triad in ASD. As regards social interaction, the ADI-R (A), there was significant negative correlation with the SSP total scores and all subscales except for low energy/weak and visual/auditory sensitivity, whereas under-responsive/seeks sensation held the highest negative correlation (p ˂ 0.008). As regards communication deficits, the ADI-R (B), there was significant negative correlation with the SSP total scores and the auditory filtering held the highest negative correlation (p ˂ 0.008), and as regards stereotypic behavior, the ADI-R (C), there was significant negative correlation with the SSP total scores and all subscales except for low energy/weak and visual/auditory sensitivity whereas taste/smell sensitivity and auditory filtering held the highest negative correlation (p ˂ 0.001). There was a high significant negative correlation between the severity of autistic symptoms (assessed by CARS) and the SSP total scores, and for all subscales (p ˂ 0.001) except for low energy/weak, the correlation was significant (not highly significant) (p ˂ 0.05). Finally, there was high percentage of parental consanguinity among the participants (80%).

Conclusions

SPD negatively affected the ASD symptom triad which highlights the importance of sensory integration therapy (SIT) as a major core of ASD treatment alongside the other treatment modalities. Early ASD screening is mandatory in families with parental consanguinity.
Characterization of focal liver lesions using sulphur hexafluoride (SF6) microbubble contrast-enhanced ultrasonography
Authors: Mansour Mohamed Gamal El-Din , Kader Mona Hussein Abdel , Arafa Hanan Mahmoud , Ali Susan Adil
Published: January 2021

Background

Focal hepatic lesions incidentally detected during ultrasound usually need further step for proper characterization. The aim of this study was to highlight the efficacy of microbubble contrast-enhanced ultrasonography (CEUS) in characterization of focal liver lesions. This prospective study was conducted on 60 patients presented with hepatic focal lesions in the period from January 2019 to June 2020. CEUS studies were performed after a baseline conventional ultrasound with the same machine by the same operator. The ultrasound contrast agent used is second-generation US contrast agent. The enhancement patterns of the hepatic lesions were studied during the vascular phases up to 5 min and the data were correlated with histopathology, triphasic contrast-enhanced CT, and clinical follow-up.

Results

CEUS demonstrated a sensitivity of 94.2%, specificity of 88.9%, positive predictive value of 91%, negative predictive value of 94.1%, and accuracy of 92.3% for characterization of hepatic focal lesions, compared to a sensitivity of 100%, specificity of 81.8%, positive predictive value of 84%, negative predictive value of 100%, and accuracy of 90.7% for triphasic CT.

Conclusion

CEUS is an effective tool in characterization of HFLs and recommended as a second diagnostic step after conventional ultrasound to immediately establish the diagnosis especially in patients with contraindications to CECT.