bluuebunny/crossref_metadata_2025 huggingface dataset:
Mapeamento e análise de áreas verdes urbanas em Paulínia (SP): estudo com a aplicação de geotecnologias
Authors: Bargos Danubia Caporusso , Matias Lindon Fonseca
Published: June 2012

As geotecnologias constituem um importante instrumental de auxílio aos estudos relacionados às áreas verdes urbanas, pois permitem o tratamento da informação espacial relacionada a estas áreas e suas especificidades de forma ágil e precisa. Este trabalho teve como objetivo o mapeamento e análise da disponibilidade de áreas verdes urbanas no município de Paulínia (SP) por meio da utilização das geotecnologias, a fim de subsidiar políticas públicas para o município. A metodologia utilizada mostrou-se eficiente para o mapeamento e análise da configuração espacial das áreas verdes urbanas do município. O mapeamento de tais áreas permitiu constatar que sua distribuição se dá de forma heterogênea, e que em sua grande maioria se apresentam na forma de pequenos fragmentos de vegetação arbórea remanescente. Os resultados indicam que o município tem apresentado uma acentuada redução da vegetação natural e, como consequência, possui uma redução das áreas verdes na área urbana.
Description of Larvae ofAnastrephaspp. (Diptera: Tephritidae) in theFraterculusGroup
Authors: Dutra Vivian S , Ronchi-Teles Beatriz , Steck Gary J , Silva Janisete G
Published: July 2012

The second- and third-instar larvae of three species in the fraterculus group, Anastrepha bahiensis Lima, Anastrepha coronilli Carrejo & González, and Anastrepha turpiniae Stone are described and illustrated for the first time. Character states that can be used to distinguish among the three species and their overlap among other species in the fraterculus group are discussed.
Seasonal Abundance and Population Structure of Chinavia hilaris and Nezara viridula (Hemiptera: Pentatomidae) in Georgia Farmscapes Containing Corn, Cotton, Peanut, and Soybean
Authors: Herbert John J , Toews Michael D
Published: July 2012

Stink bugs are economically important pests that damage a wide range of crops in the southeastern United States. Stink bug feeding on developing cotton (Gossypium hirsutum L. ) bolls may result in reduced yield and loss of fiber quality; similarly, feeding on developing soybean [Glycine max (L.) Merr.] pods can reduce yield and seed quality. During 2009 and 2010, the seasonal abundance and reproductive biology of Chinavia hilaris (Say) and Nezara viridula (L.) were investigated in replicated 1.62–2.83-ha farmscapes containing equal proportions of corn (Zea mays L.), cotton, peanut (Arachis hypogaea L.), and soybean. Crops were sampled weekly by using whole plant examinations in corn and sweep net sampling in cotton, peanut, and soybean. In 2010 only, adults were dissected to rate their reproductive development, and nymphs were classified to instar. No C. hilaris adults or nymphs ever were observed in corn; however, nymphs were observed in cotton and soybean during late September with peak abundance occurring just after the autumnal equinox. The peak of late-instar nymphs was followed within 2 wk by a peak of nonreproductive adults. More adults were observed in soybean than cotton. In contrast, N. viridula nymphs and adults were found across all crops and had multiple generations throughout the growing season. Results from this study indicate that C. hilaris and N. viridula are different in voltinism, phenology, and use of hosts. These data provide knowledge of stink bug biology and population ecology at the landscape level and are useful for designing and implementing stink bug management programs.
DNA Barcodes and Targeted Sampling Methods Identify a New Species and Cryptic Patterns of Host Specialization Among North American Coptera (Hymenoptera: Diapriidae)
Authors: Forbes Andrew A , Satar Serdar , Hamerlinck Gabriela , Nelson Amanda E , Smith James J
Published: July 2012

Fewer than half of the 80–100 North American species in parasitoid genus Coptera Say (Hymenoptera: Diapriidae) have been described. Hosts are known for just nine of these. The taxonomy of Coptera has been complicated by its cryptic morphology and a life history that includes parasitism of pupae beneath the surface of soils. Here, we describe collections targeting the host genus with which Coptera have most frequently been associated: flies in genus Rhagoletis (Loew) (Diptera: Tephritidae). DNA barcodes, morphology, and ecology (host associations) were used to understand species limits for Coptera collected from Rhagoletis. Four species of Coptera were recovered from five species of Rhagoletis, including a new species: Coptera n. sp. 1. Two of the associations with particular species of Rhagoletis were previously unknown, and no two species of Coptera were found to be attacking the same host, suggesting these four Coptera are specialist parasites. As several of the 25 North American species of Rhagoletis are agricultural pests, a better understanding of their natural associations with Coptera may prove valuable to biological control efforts.
Tillage Affects Imazamox Carryover in Yellow Mustard
Authors: Rood Jonquil , Campbell Joan , Thill Donn , Ball Dan , Bennett Larry , Yenish Joseph , Nelson John , Rood Rod , Shafii Bahman , Price William
Published: July 2012

Farmers grow crops in the dryland region of the Pacific Northwest (PNW) using tillage practices ranging from moldboard plowing to no-tillage. The objective of this study was to determine the effect of tillage on persistence of imazamox herbicide in intermediate and high precipitation zones of the inland PNW. Along with a nontreated control, imazamox was applied to imidazolinone-tolerant winter wheat in the fall and spring at one, two, and three times the maximum labeled rate at locations near Genesee, ID, Davenport, WA, and Pendleton, OR. Moldboard plow, chisel plow, and no-till tillage treatments were implemented soon after wheat harvest and yellow mustard was planted the following season to determine crop response. Experiments were conducted at each location in 2005 to 2007 and 2006 to 2008. There were significant location by year and year and location interactions. There was no significant tillage by imazamox rate interaction, except at Pendleton in year 2, for all measured yellow mustard responses (crop injury, biomass, and yield). Genesee was colder than Pendleton and had more precipitation than Davenport, resulting in more injury to yellow mustard at Genesee than at Pendleton but less than at Davenport. Davenport had greater injury than the other two locations, likely due to lower soil pH, higher organic matter (OM), and cooler, drier climate, which allowed imazamox to persist longer in the soil. Overall, Pendleton had the least yellow mustard injury, which likely was related to its warmer, wetter climate and the concomitant rapid soil dissipation of imazamox. Tillage did not reduce the persistence of imazamox. Yellow mustard had the lowest injury and had greater mature biomass and seed yield in no-till seeded plots when averaged across imazamox rates compared to moldboard and chisel-plowed plots.
Purple Nutsedge (Cyperus rotundus) Control in Bermudagrass Turf with Imazosulfuron
Authors: Henry Gerald M. , Sladek Bradley S. , Hephner Andrew J. , Cooper Tyler
Published: July 2012

Purple nutsedge response to various rates and timings of imazosulfuron was evaluated in 2007 and 2008 in Abilene, TX. Bermudagrass phytotoxicity never exceeded 4% throughout the duration of the trial and all bermudagrass recovered within 7 d of herbicide application. Imazosulfuron (0.56 kg ai ha−1) followed by (fb) imazosulfuron 1 wk after initial treatment (WAIT), imazosulfuron at 1.12 kg ai ha−1, and trifloxysulfuron at 0.03 kg ai ha−1exhibited 94 to 96% control 4 WAIT. Imazosulfuron (0.56 kg ai ha−1) fb imazosulfuron 2, 3, and 4 WAIT exhibited 99% control 4 WAIT. Eight weeks later (12 WAIT), imazosulfuron (0.56 kg ai ha−1) fb imazosulfuron 3 WAIT controlled purple nutsedge 91%, whereas similar control (82 to 84%) was observed with a single application of trifloxysulfuron and imazosulfuron (0.56 kg ai ha−1) fb imazosulfuron 2 and 4 WAIT. A single application of imazosulfuron at 1.12 kg ai ha−1and sequential treatment with imazosulfuron (0.56 kg ai ha−1) on a 1-wk interval only controlled purple nutsedge 51 to 69% 12 WAIT. Timing of sequential imazosulfuron application was identified as an important component of the purple nutsedge control program. Waiting 2, 3, or 4 WAIT for sequential imazosulfuron applications, rather than 1 WAIT, increased purple nutsedge control 31 to 40% 12 WAIT. The highest level of purple nutsedge control (91%) was observed with applications of imazosulfuron (0.56 kg ai ha−1) fb imazosulfuron 3 WAIT applied during midsummer. However, control with this treatment was statistically similar to control with a single application of trifloxysulfuron (82%).
Texasweed (Caperonia palustris) Interference in Drill-Seeded Rice
Authors: Godara Rakesh K. , Williams Billy J. , Webster Eric P. , Griffin James L. , Geaghan James P.
Published: July 2012

Field research was conducted near Saint Joseph, LA, in 2008 and 2009 to evaluate Texasweed interference in drill-seeded rice. Season-long Texasweed interference at 1 plant m−2was estimated to cause 5% yield loss. Yield loss from 10 and 50 plants m−2was 31 and 61%, respectively. Yield loss was primarily due to a reduction in effective tillers per square meter. Thousand-grain weight of rice was not affected by season-long Texasweed interference. Path analysis indicated yield component compensation, i.e., a reduction in effective tillers per square meter probably caused an increase in grains per panicle. However, that effect was not strong enough to reverse the detrimental effect of reduced effective tillers per square meter on rice yield. The critical period of Texasweed interference to cause more than 5% yield loss was estimated to be between 0 and 6 wk after rice emergence.
Impact of Drift Rates of Imazethapyr and Low Carrier Volume on Non-Clearfield Rice
Authors: Hensley Justin B. , Webster Eric P. , Blouin David C. , Harrell Dustin L. , Bond Jason A.
Published: July 2012

Field studies were conducted near Crowley, LA, in 2005 through 2007 to evaluate the effects of simulated herbicide drift on ‘Cocodrie’ rice. Each application was made with the spray volume varying proportionally to herbicide dosage based on a constant spray volume of 234 L ha−1and an imazethapyr rate of 70 g ai ha−1. The 6.3%, 4.4 g ha−1, herbicide rate was applied at a spray volume of 15 L ha−1and the 12.5%, 8.7 g ha−1, herbicide rate was applied at a spray volume of 29 L ha−1. An application of imazethapyr at one-tiller, panicle differentiation (PD), and boot resulted in increased crop injury compared with the nontreated rice. The most injury observed occurred on rice treated at the one-tiller timing. Imazethapyr at one-tiller, PD, and boot reduced plant height at harvest and primary and total (primary plus ratoon) crop yield, with the greatest reduction in primary crop yield resulting from imazethapyr applied at boot. Imazethapyr did not affect rice treated at primary crop maturity.
Effect of Postflood Quinclorac Applications on Commercial Rice Cultivars
Authors: Bond Jason A. , Walker Timothy W.
Published: July 2012

Rice cultivar, growth stage at application, or both may influence rice tolerance to quinclorac. Field studies were conducted to compare the response of five rice cultivars ‘Bowman’, ‘Cheniere’, ‘CL161’, ‘Cocodrie’, and ‘XL723’ to postflood quinclorac applications. Quinclorac at 0.56 kg ai ha−1was applied 2 and 4 wk after flood (WAF). Pooled across quinclorac application timings, no differences in maturity were detected among the cultivars in 2008, but maturity of Cheniere and XL723 were delayed compared with CL161 and Cocodrie in 2007. Maturity of Cheniere and XL723 was delayed in 2007 compared with 2008. Pooled over cultivar, maturity was similar for 2 and 4 WAF applications in 2007 but was delayed for 2 WAF treatments in 2008. Regardless of year, postflood quinclorac applications reduced rough rice yield of all cultivars except Bowman. Cheniere and XL723 had lower rough rice yields compared with other cultivars in 2007; however, in 2008, rough rice yields of Cheniere, CL161, Cocodrie, and XL723 were similar, but still lower, than that of Bowman. Pooled over cultivar, postflood quinclorac reduced rough rice yields more when applied 4 WAF than at 2 WAF during both years. Our results demonstrate that Cheniere and XL723 are less tolerant than Bowman is to postflood quinclorac applications and that all evaluated cultivars are more susceptible to quinclorac applied at later developmental stages. Consequently, if circumstances necessitate a postflood quinclorac application, the herbicide should be applied no later than panicle initiation and should not be applied to Cheniere or XL723.
Effect of High-Dose Erythropoietin on Graft Function after Kidney Transplantation
Authors: Sureshkumar Kalathil K. , Hussain Sabiha M. , Ko Tina Y. , Thai Ngoc L. , Marcus Richard J.
Published: June 2012

Background and objectives

Delayed graft function (DGF) is associated with adverse long-term outcomes after deceased-donor kidney (DDK) transplantation. Ischemia-reperfusion injury plays a crucial role in the development of DGF. On the basis of promising animal data, this study evaluated any potential benefits of erythropoietin-alfa (EPO-α) given intra-arterially at the time of reperfusion of renal allograft on the degree of allograft function, as well as tubular cell injury measured by urinary biomarkers in the early post-transplant period.

Design, setting, participants, & measurements

A prospective, randomized, double-blind, placebo-controlled clinical trial was conducted to evaluate the influence of EPO-α administered intraoperatively on the outcomes of DDK transplantations performed at the study center between March 2007 and July 2009.

Results

Seventy-two patients were randomly assigned to EPO-α (n=36) or placebo (n=36). The incidences of DGF, slow graft function, and immediate graft function did not significantly differ between the treatment and control groups (41.7% versus 47.2%, 25.0% versus 36.1%, and 33.3% versus 16.7%, respectively; P=0.24). The groups had similar levels of urinary biomarkers, including neutrophil gelatinase-associated lipocalin and IL-18 at multiple times points soon after transplantation; urinary output during the first 3 postoperative days; 1-month renal function; and BP readings, hemoglobin, and adverse effects during the first month.

Conclusions

This study did not show any clinically demonstrable beneficial effects of high-dose EPO-α given intra-arterially during the early reperfusion phase in DDK transplant recipients in terms of reducing the incidence of DGF or improving short-term allograft function.
Urolithiasis and the Risk of ESRD
Authors: El-Zoghby Ziad M. , Lieske John C. , Foley Robert N. , Bergstralh Eric J. , Li Xujian , Melton L. Joseph , Krambeck Amy E. , Rule Andrew D.
Published: June 2012

Background and objectives

The contribution of urolithiasis, if any, to the development of ESRD is unclear.

Design, setting, participants, & measurements

All stone formers in Olmsted County, Minnesota, first diagnosed between 1984 and 2008 were identified by diagnostic codes with up to four controls matched on age and sex. Charts were reviewed to validate symptomatic stone formers in a random subset. Incident ESRD events were identified by the US Renal Data System.

Results

Altogether, 51 stone formers and 75 controls developed ESRD among 6926 stone formers and 24,620 matched controls followed for a mean of 9 years. Stone formers had an increased risk of ESRD after adjusting for diabetes, hypertension, dyslipidemia, gout, and CKD (hazard ratio: 2.09; 95% confidence interval: 1.45–3.01). This increased risk of ESRD remained in the subset of 2457 validated symptomatic stone formers (hazard ratio: 1.95; 95% confidence interval: 1.09–3.49). The attributable risk of ESRD from symptomatic urolithiasis was 5.1% based on a prevalence of 5.4% for stone formers. For stone formers versus controls who developed ESRD, there was an increased likelihood of past hydronephrosis (44% versus 4%), recurrent urinary tract infections (26% versus 4%), acquired single kidney (15% versus 3%), neurogenic bladder (12% versus 1%), and ileal conduit (9% versus 0%), but not diabetes (32% versus 49%) or hypertension (44% versus 52%).

Conclusions

Symptomatic stone formers are at increased risk for ESRD independent of several cardiovascular risk factors. Other urological disease is relatively common among stone formers who develop ESRD.
Lie symmetries and Noether symmetries
Authors: Leach P.G.L.
Published: July 2012

We demonstrate that so-called nonnoetherian symmetries with which a known
first integral is associated of a differential equation derived from a
Lagrangian are in fact noetherian. The source of the misunderstanding lies
in the nonuniqueness of the Lagrangian.
Extensions of Euler harmonic sums
Authors: Sofo Anthony , Cvijovic Djurdje
Published: July 2012

Three new closed-form summation formulae involving harmonic numbers are
established using simple arguments and they are very general extensions of
Euler's famous harmonic sum identity. Some illustrative special cases as well
as immediate consequences of the main results are also considered.
Development and validation of novel RP-UPLC method for estimation of atropine sulphate in pharmaceutical dosage form
Authors: Arvadiya A.C. , Dahivelker P.P.
Published: July 2012

A simple, precise, accurate, sensitive and repeatable RP-UPLC method was
developed for quantitative determination of atropine sulphate in
pharmaceutical dosage form. The method was developed by using C18 column
Hiber HR Purospher Star (100mm?2.1mm id, 2?m particle size) as stationary
phase with Phosphate Buffer: Acetonitrile (87:13, %v/v) as a mobile phase, pH
was adjusted to 3.5 by ortho-phosphoric acid at a flow rate of 0.5 mL/min and
column temperature maintained at 30?C. Quantification of eluted compound was
achieved with PDA detector at 210 nm. Atropine sulphate followed linearity in
concentration range of 2.5-17.5 ?g/mL with r2=0.9998 (n=6). Limit of
detection (LOD) and limit of quantification (LOQ) values were 0.0033 and
0.0102 ?g/mL for atropine sulphate. The validation study is carried out as
per International Conference on Harmonization (ICH) guidelines. This method
was successfully applied for estimation of atropine sulphate in
pharmaceutical formulation.
Maternal High-Fat Diet During Gestation or Suckling Differentially Affects Offspring Leptin Sensitivity and Obesity
Authors: Sun Bo , Purcell Ryan H. , Terrillion Chantelle E. , Yan Jianqun , Moran Timothy H. , Tamashiro Kellie L.K.
Published: June 2012

Maternal high-fat (HF) diet throughout gestation and suckling has long-term consequences on the offspring’s metabolic phenotype. Here we determine the relative contribution of pre- or postnatal maternal HF diet on offspring’s metabolic phenotype. Pregnant Sprague-Dawley rats were maintained on normal chow or HF diet throughout gestation and suckling. All litters were cross-fostered to chow or HF dams on postnatal day (PND)1, resulting in four groups. Body weight, body composition, and glucose tolerance were measured at weaning and in adulthood. Leptin sensitivity was assessed by signal transducer and activator of transcription (STAT)3 activation on PND10 and PND21. Pups cross-fostered to HF dams gained more body weight than chow pups by PND7 and persisted until weaning. Postnatal HF pups had greater adiposity, higher plasma leptin concentration, impaired glucose tolerance, and reduced phosphorylated STAT3 in response to leptin in the arcuate nucleus at weaning. After weaning, male offspring cross-fostered to HF dams were hyperphagic and maintained greater body weight than postnatal chow pups. Postnatal HF diet during suckling continued to impair glucose tolerance in male and female offspring in adulthood. Maternal HF diet during suckling has a greater influence in determining offspring’s metabolic phenotype than prenatal HF diet exposure and could provide insight regarding optimal perinatal nutrition for mothers and children.
Impaired MEK Signaling and SERCA Expression Promote ER Stress and Apoptosis in Insulin-Resistant Macrophages and Are Reversed by Exenatide Treatment
Authors: Liang Chien-Ping , Han Seongah , Li Gang , Tabas Ira , Tall Alan R.
Published: June 2012

Accumulation of toxic lipids evokes the unfolded protein response (UPR) and apoptotic death of macrophages and vascular cells in atherosclerotic plaques. Primary macrophages from insulin-resistant ob/ob and insulin receptor (Insr)−/− mice display increased apoptosis in response to loading with free cholesterol or oxysterol, but underlying mechanisms have not been elucidated. We show increased activation of all three major branches of the UPR in response to free cholesterol or oxysterol loading in insulin-resistant macrophages. Inhibition and rescue experiments revealed that defective MEK/extracellular signal\x{2013}related kinase (ERK)/cAMP-responsive element–binding protein (CREBP) signaling in insulin-resistant macrophages leads to decreased expression of sarcoplasmic endoplasmic reticulum (ER) Ca2+-ATPase, depletion of ER calcium stores, PKR-like ER kinase activation, and ER stress–associated apoptosis. Activation of macrophage glucagon-like peptide 1 (GLP-1) receptor via the antidiabetic drug exenatide led to improvements in both ERK and AKT signaling and reversed the increase in UPR and apoptosis of insulin-resistant macrophages in atherosclerotic lesions of ob/ob.Ldlr−/− and Insr−/−.Ldlr−/− mice. Increased signaling via GLP-1 receptor or the CREBP activator protein kinase A thus offers a way to rescue insulin-resistant macrophages from excessive ER stress responses and apoptosis in insulin resistance and type 2 diabetes.
Toll-like Receptor 4 Deficiency Promotes the Alternative Activation of Adipose Tissue Macrophages
Authors: Orr Jeb S. , Puglisi Michael J. , Ellacott Kate L.J. , Lumeng Carey N. , Wasserman David H. , Hasty Alyssa H.
Published: June 2012

Obesity is characterized by adipose tissue (AT) macrophage (ATM) accumulation, which promotes AT inflammation and dysfunction. Toll-like receptor 4 (TLR4) deficiency attenuates AT inflammation in obesity but does not impede the accumulation of ATMs. The purpose of the current study was to determine whether TLR4 deficiency alters ATM polarization. TLR4−/− and wild-type mice were fed a low-fat, high-monounsaturated fat (HFMUFA), or a high-saturated fat (HFSFA) diet for 16 weeks. Further, we used a bone marrow transplant model to determine the influence of hematopoietic cell TLR4 signaling. The metabolic and inflammatory responses to high-fat feeding and ATM phenotype were assessed. Global and hematopoietic cell TLR4 deficiency, irrespective of recipient genotype, produced a shift in ATM phenotype toward an alternatively activated state, which was accompanied by reduced AT inflammation. Despite the observed shift in ATM phenotype, neither global nor hematopoietic cell TLR4 deficiency influenced systemic insulin sensitivity after high-fat feeding. Results of the current study suggest that TLR4 directly influences ATM polarization but question the relevance of TLR4 signaling to systemic glucose homeostasis in obesity.
Adoptive Transfer of Immunomodulatory M2 Macrophages Prevents Type 1 Diabetes in NOD Mice
Authors: Parsa Roham , Andresen Pernilla , Gillett Alan , Mia Sohel , Zhang Xing-Mei , Mayans Sofia , Holmberg Dan , Harris Robert A.
Published: June 2012

Macrophages are multifunctional immune cells that may either drive or modulate disease pathogenesis depending on their activation phenotype. Autoimmune type 1 diabetes (T1D) is a chronic proinflammatory condition characterized by unresolved destruction of pancreatic islets. Adoptive cell transfer of macrophages with immunosuppressive properties represents a novel immunotherapy for treatment of such chronic autoimmune diseases. We used a panel of cytokines and other stimuli to discern the most effective regimen for in vitro induction of immunosuppressive macrophages (M2r) and determined interleukin (IL)-4/IL-10/transforming growth factor-β (TGF-β) to be optimal. M2r cells expressed programmed cell death 1 ligand-2, fragment crystallizable region γ receptor IIb, IL-10, and TGF-β, had a potent deactivating effect on proinflammatory lipopolysaccharide/interferon-γ–stimulated macrophages, and significantly suppressed T-cell proliferation. Clinical therapeutic efficacy was assessed after adoptive transfer in NOD T1D mice, and after a single transfer of M2r macrophages, >80% of treated NOD mice were protected against T1D for at least 3 months, even when transfer was conducted just prior to clinical onset. Fluorescent imaging analyses revealed that adoptively transferred M2r macrophages specifically homed to the inflamed pancreas, promoting β-cell survival. We suggest that M2r macrophage therapy represents a novel intervention that stops ongoing autoimmune T1D and may have relevance in a clinical setting.
Mechanisms Underlying Antigen-Specific Tolerance of Stable and Convertible Th17 Cells During Suppression of Autoimmune Diabetes
Authors: Wan Xiaoxiao , Guloglu F. Betul , VanMorlan Amie M. , Rowland Linda M. , Jain Renu , Haymaker Cara L. , Cascio Jason A. , Dhakal Mermagya , Hoeman Christine M. , Tartar Danielle M. , Zaghouani Habib
Published: June 2012

Type 1 diabetes involves both T helper (Th)1 and Th17 cells. While the mechanisms underlying the control of Th1 cells are relatively well defined, those operating modulation of Th17 cells remain unknown. Moreover, given that Th17 cells are plastic and can drive disease as stable or convertible T cells, effective approaches to counter type 1 diabetes would have to alter Th17 function under both circumstances. Herein, we genetically incorporated the BDC2.5-reactive p79 mimotope into an Ig molecule, and the resulting Ig-p79 was used to investigate Th17 tolerance. Accordingly, diabetogenic BDC2.5 Th17 cells were transferred into NOD mice under convertible or stable conditions and their fate was evaluated upon induction of tolerance and disease suppression by Ig-p79. The findings show that convertible (Th17 to Th1) cells display downregulation of the chemokine (C-X-C motif) receptor 3 that was associated with diminished T-box transcription factor T-bet expression, retention in the spleen, and inhibition of trafficking to the pancreas. In contrast, stable Th17 cells downregulated orphan nuclear receptor ROR-γt but increased Fas ligand expression and died by apoptosis. Thus, the final signature transcription factor shapes the mechanism of tolerance in plastic Th17 cells. These findings suggest that effective strategies against type 1 diabetes will require regimens that could drive both mechanisms of tolerance to overcome the disease.
Human β-Cell Proliferation and Intracellular Signaling
Authors: Kulkarni Rohit N. , Mizrachi Ernesto-Bernal , Ocana Adolfo Garcia , Stewart Andrew F.
Published: June 2012

A major goal in diabetes research is to find ways to enhance the mass and function of insulin secreting β-cells in the endocrine pancreas to prevent and/or delay the onset or even reverse overt diabetes. In this Perspectives in Diabetes article, we highlight the contrast between the relatively large body of information that is available in regard to signaling pathways, proteins, and mechanisms that together provide a road map for efforts to regenerate β-cells in rodents versus the scant information in human β-cells. To reverse the state of ignorance regarding human β-cell signaling, we suggest a series of questions for consideration by the scientific community to construct a human β-cell proliferation road map. The hope is that the knowledge from the new studies will allow the community to move faster towards developing therapeutic approaches to enhance human β-cell mass in the long-term goal of preventing and/or curing type 1 and type 2 diabetes.
Long-Term Remission of Diabetes in NOD Mice Is Induced by Nondepleting Anti-CD4 and Anti-CD8 Antibodies
Authors: Yi Zuoan , Diz Ramiro , Martin Aaron J. , Morillon Yves Maurice , Kline Douglas E. , Li Li , Wang Bo , Tisch Roland
Published: June 2012

Residual β-cells found at the time of clinical onset of type 1 diabetes are sufficient to control hyperglycemia if rescued from ongoing autoimmune destruction. The challenge, however, is to develop an immunotherapy that not only selectively suppresses the diabetogenic response and efficiently reverses diabetes, but also establishes long-term β-cell–specific tolerance to maintain remission. In the current study, we show that a short course of nondepleting antibodies (Abs) specific for the CD4 and CD8 coreceptors rapidly reversed clinical disease in recent-onset diabetic NOD mice. Once established, remission was maintained indefinitely and immunity to foreign antigens unimpaired. Induction of remission involved selective T-cell purging of the pancreas and draining pancreatic lymph nodes and upregulation of transforming growth factor (TGF)-β1 by pancreas-resident antigen-presenting cells. Neutralization of TGF-β blocked the induction of remission. In contrast, maintenance of remission was associated with tissue-specific immunoregulatory T cells. These findings demonstrate that the use of nondepleting Ab specific for CD4 and CD8 is a robust approach to establish long-term β-cell–specific T-cell tolerance at the onset of clinical diabetes.
HLA-B7–Restricted Islet Epitopes Are Differentially Recognized in Type 1 Diabetic Children and Adults and Form Weak Peptide-HLA Complexes
Authors: Scotto Matthieu , Afonso Georgia , Østerbye Thomas , Larger Etienne , Luce Sandrine , Raverdy Cécile , Novelli Giulia , Bruno Graziella , Gonfroy-Leymarie Céline , Launay Odile , Lemonnier François A. , Buus Søren , Carel Jean-Claude , Boitard Christian , Mallone Roberto
Published: June 2012

The cartography of β-cell epitopes targeted by CD8+ T cells in type 1 diabetic (T1D) patients remains largely confined to the common HLA-A2 restriction. We aimed to identify β-cell epitopes restricted by the HLA-B7 (B*07:02) molecule, which is associated with mild T1D protection. Using DNA immunization on HLA-B7–transgenic mice and prediction algorithms, we identified GAD and preproinsulin candidate epitopes. Interferon-γ (IFN-γ) enzyme-linked immunospot assays on peripheral blood mononuclear cells showed that most candidates were recognized by new-onset T1D patients, but not by type 2 diabetic and healthy subjects. Some epitopes were highly immunodominant and specific to either T1D children (GAD530–538; 44% T cell–positive patients) or adults (GAD311–320; 38%). All epitopes displayed weak binding affinity and stability for HLA-B7 compared with HLA-A2–restricted ones, a general feature of HLA-B7. Single-cell PCR analysis on β-cell–specific (HLA-B7 tetramer–positive) T cells revealed uniform IFN-γ and transforming growth factor-β (TGF-β) mRNA expression, different from HLA-A2–restricted T cells. We conclude that HLA-B7–restricted islet epitopes display weak HLA-binding profiles, are different in T1D children and adults, and are recognized by IFN-γ+TGF-β+CD8+ T cells. These features may explain the T1D-protective effect of HLA-B7. The novel epitopes identified should find valuable applications for immune staging of HLA-B7+ individuals.
Effects of Aerobic Versus Resistance Exercise Without Caloric Restriction on Abdominal Fat, Intrahepatic Lipid, and Insulin Sensitivity in Obese Adolescent Boys
Authors: Lee SoJung , Bacha Fida , Hannon Tamara , Kuk Jennifer L. , Boesch Chris , Arslanian Silva
Published: June 2012

The optimal exercise modality for reductions of abdominal obesity and risk factors for type 2 diabetes in youth is unknown. We examined the effects of aerobic exercise (AE) versus resistance exercise (RE) without caloric restriction on abdominal adiposity, ectopic fat, and insulin sensitivity and secretion in youth. Forty-five obese adolescent boys were randomly assigned to one of three 3-month interventions: AE, RE, or a nonexercising control. Abdominal fat was assessed by magnetic resonance imaging, and intrahepatic lipid and intramyocellular lipid were assessed by proton magnetic resonance spectroscopy. Insulin sensitivity and secretion were evaluated by a 3-h hyperinsulinemic-euglycemic clamp and a 2-h hyperglycemic clamp. Both AE and RE prevented the significant weight gain that was observed in controls. Compared with controls, significant reductions in total and visceral fat and intrahepatic lipid were observed in both exercise groups. Compared with controls, a significant improvement in insulin sensitivity (27%) was observed in the RE group. Collapsed across groups, changes in visceral fat were associated with changes in intrahepatic lipid (r = 0.72) and insulin sensitivity (r = −0.47). Both AE and RE alone are effective for reducing abdominal fat and intrahepatic lipid in obese adolescent boys. RE but not AE is also associated with significant improvements in insulin sensitivity.
Cultivating Flourishing Lives
Authors: Grant Carl A.
Published: June 2012

Presented at AERA 2010 as the Social Justice Award Lecture, this article calls attention to the purposes of education in the 21st century and the need for a robust, social justice vision of education. Here, it is argued that education is about the cultivation of a flourishing life and not only the narrow preparation for employment. To realize education that cultivates students’ flourishing minds and lives, this article proposes five core principles for this robust social justice vision: self-assessment, critical questioning, practicing democracy, social action, and criteria for adjudication.
Phase diagram of the Al–Dy–Zr ternary system at 773 K
Authors: Hu Zhaohua , Zhan Yongzhong , Ling Maoxing , Liang Yong , Liu Ya , Wei Shuangjie , Du Yong
Published: July 2012

X-ray powder diffractometry, scanning electron microscopy, optical microscopy and differential thermal analysis were used for constructing the isothermal section of the Al–Dy–Zr system in the whole compositional range at 773 K. The existence of 13 binary compounds and 1 ternary compound, i.e. Al3Zr, Al2Zr, Al3Zr2, AlZr, Al3Zr4, Al2Zr3, AlZr2, AlZr3, α - Al3Dy, Al2Dy, AlDy, Al2Dy3, AlDy2, and Al30Dy7Zr3 were confirmed. The ternary compound Al30Dy7Zr3 has AuCu3 structure (space group Pm3m) with lattice parameter of a = 0.4206 nm. The isothermal section consists of 17 single-phase regions, 32 binary-phase regions and 16 ternary-phase regions. The homogeneity of Dy in the binary compounds Al3Zr, Al2Zr, Al3Zr2, and AlZr2 is determined to be 2 at.%, 3 at.%, 4 at.%, and 2 at.% Dy, respectively. The homogeneity range of Dy in Zr is up to v7 at.% Zr. The solid solubility of Zr in the binary compounds Al3Dy, Al2Dy, and AlDy is determined to be 2 at.%, 26 at.%, and 3 at.% Zr, respectively.
Socioeconomic Analysis of Alternative Farming Systems in Improving Livelihood Security of Small Farmers in Selected Areas of Bangladesh
Authors: Sharmin Sadika , Islam M. Serajul , Hasan Md. Kamrul
Published: July 2012

The present study attempted to determine the economic returns of alternative farming system of integrated farming aiming to address the livelihood security of small farm households. One hundred and sixty small and medium farmers practicing in four different FS such as Crop-Poultry (C-P), Crop-Poultry-Pond fisheries (C-P-F), Crop-Livestock-Poultry (C-L-P) and Crop-Livestock-Poultry-Pond fisheries (C-L-P-F) were selected from Mymensingh, Jamalpur and Sherpur district. The main information collected were socio-demographic characteristics of farm households, household income from farm and non-farm sources, size of land holding and farming system followed, livestock and poultry rearing and pond fish culture, and effect of integrated farming on household income and livelihood security of small farm households. Data were collected covering one production period during 2009-2010. For estimating farm income of small farm household, whole farm approach was considered. Increased number of farm enterprises caused higher farm income. However, considering the small farm practices and whole farm approach, the farm income as well as net return was higher for C-L-P-F farmers (Tk. 57002), followed by C-L-P (Tk. 52978), C-P-F (Tk. 20447) and C-P (Tk. 13734) farmers. Some of the most commonly used factors in the assessment of livelihood security included those related to household income and expenditure, expenditure spent on food, adequacy of food taken, household structure and facilities, owning household assets, and access to drinking water and sanitation, and other basic needs of farm household. All these socioeconomic factors were found to be related livelihood security of small farmers in Bangladesh. Women were more involved in income earning activities and household decision making with the introduction of integrated farming in small farm condition.DOI: http://dx.doi.org/10.3329/agric.v10i1.11065The Agriculturists 2012; 10(1): 51-63
Association of Serum Testosterone with Acne Vulgaris in Women
Authors: Rahman Md. Moksedur , Sikder Md. Akram Ullah , Rashid Muhammad Munir , Khondker Lubna , Hazra Samaresh Chandra , Nessa Moriom
Published: June 2012

Background: Androgens enhance the sebum production and follicular keratosis that plays the key role in the aetiology of acne. Objective: To find out the association between serum testosterone and acne vulgaris. Methods: A case control study was carried out for a period of two years in the outpatient department of Dermatology and Venereology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh. Female patients having acne vulgaris were selected as case. Healthy control (age and sex matched) were enrolled from the community. Results: The study showed that the mean age of the cases was 22.43 with standard deviation 5.2 years and the mean age of the control was 23.23 with standard deviation 5.9 years. The mean duration of disease was 62.6 months ranging from 12 months to 300 months. All the patients had presented with comedones (blackheads and whiteheads) followed by 94.3% had papules and 58.6% had pustules. Considering the site of lesion, all the patients had acne in the face. Data analysis revealed that the percentage of serum testosterone above normal was found to be high among the cases with acne (10%) whereas below normal level of serum testosterone was found among the control and the difference was statistically significant (p<0.001). Conclusion:The study found a significant association between serum testosterone and acne vulgaris. As serum testosterone is associated with acne vulgaris, testosterone levels should be measured in patients presenting with acne vulgaris especially in treatment resistant cases and anti-androgen treatment may be indicated in cases with elevated testosterone level.DOI: http://dx.doi.org/10.3329/bsmmuj.v5i1.10980 BSMMU J 2012; 5(1):1-5
Treatment of Scoliosis by Hartshill Rectangle and Sublaminar Wiring
Authors: Ahsan Md. Kamrul , Sakeb Najmus , Zaman Naznin , Mahmud Abdullah Al
Published: June 2012

Background: Scoliosis is a complex three dimensional deformity characterized by coronal, sagittal and horizontal plane deviation. Treatment of scoliosis has made significant development in techniques and modalities of internal fixation. Objective: To see the efficacy of Hartshill rectangle and sublaminar wiring for correction of scoliotic deformity, clinical outcome and patients satisfaction. Methods: During the period of July 2008 to June 2011, 14 patients of scoliotic spinal deformities of various etiologies (Congenital-02, idiopathic-12) were operated. Age ranged from 11 to 23 years with mean 16.71 ± 03.22 years. Female 09 and male 05 patients were included where Hartshill rectangles with sublaminar wiring were used for every case. Results: At least 12 months (range 12-36 months) follow up regarding clinico-radiological evaluation and patients satisfaction were accounted as per the SRS-24 questionnaire. The mean scoliosis in the coronal plane (Cobbs angle) measured 53.50o ± 03.98o preoperatively reducing significantly to 21.14o ± 02.77o (p<0.05) and the mean preoperative sagittal plane angle 60.14o ± 07.30o reducing significantly to average 42.14o ± 05.28o (p<0.05) at 1 year as determined by paired t-test. No neurological complication was seen except transient hyperesthesia in 02 patients, superficial wound infection in 01 patient and 01 wire breakage. All the patients were happy to live with their present back status. Conclusion: Overall outcome in segmental spinal fixation with sublaminar wire and Hartshill rectangle is satisfactory and effective.DOI: http://dx.doi.org/10.3329/bsmmuj.v5i1.10981 BSMMU J 2012; 5(1):6-11
Outcome of Pregnancy in Lupus
Authors: Sayeeda Syeda , Begum Firoza , Akhtar Nahreen , Sharmin Farzana
Published: June 2012

Background: Pregnancy in patients with SLE is associated with a high risk of maternal disease exacerbation in active disease state and adverse fetal outcome. Objective: To analyse maternal and fetal outcome in pregnant lupus patients as well as to identify influence of disease activity on it. Methods: This retrospective observational cross sectional study was done on 27 pregnant lupus patients in feto-maternal medicine wing of the department of Obstetrics and Gynaecology, BSMMU from April 2007 to March 2011. For statistical analysis z test of proportion and students t test was used. Results: Mean age of the patients was 26.6 years. At booking, 8 (29.63%) had active lupus while 19 (70.37%) was in remission. As complications of SLE, lupus flare was found in 11.1%, lupus nephritis in 25.9% and skin rash in 7.4%. Regarding obstetric complications, PIH was developed in 3.7% and preeclampsia in 11.1% of the patients. Average gestational age at delivery was 34.8 weeks. Birth weight was <2.5kg in 45.8% of neonates. There was IUGR in 33.3% of the cases and 25% of the neonates needed admission in neonatal ICU. There was no neonatal death and none of the 24 neonates had neonatal lupus. In this study there was no maternal mortality. Patients who were in active disease state, most (55.5%) delivered preterm, spontaneous abortion occurred in two and IUD in one of the patients. Average birth weight was lower (1.9+.47kg) in patients with active SLE than those of with remission (2.3+.68kg). Lupus flare developed in 66.6% with active disease, while in 33.3% with remission. Conclusion: Pregnancy is relatively safe with SLE in remission but considered as high risk in terms of fetal loss and spontaneous abortion. Disease activity influences pregnancy outcome.DOI: http://dx.doi.org/10.3329/bsmmuj.v5i1.10995 BSMMU J 2012; 5(1):18-23
Prevalence of Behavioral and Emotional Disorders among the Orphans and Factors Associated with these Disorders
Authors: Rahman Wasima , Mullick M S I , Pathan Mohammed Asraful Siddike , Chowdhury Nafia Farzana , Shahidullah Mohammed , Ahmed Helaluddin , Roy Surajit , Mazumder Atiqul Haq , Rahman Farzana
Published: June 2012

Background: Orphans are the special group of children who are generally deprived and prone to develop psychiatric disorders even reared in well run institution. Objectives: To find out the prevalence of the behavioral and emotional disorder among the children living in orphanage in Dhaka city, and to assess the possible factors associated with the presence of disorders among this study population. Methods: It was a cross sectional descriptive study conducted in selected orphanages. A Total 342 cases were included. One stage structured assessment of psychopathology was carried out by using a valid Bangla version of DAWBA (The Development and Well-Being Assessment). Data analysis was done by SPSS for windows 16.0 version. Results: The results indicate that overall prevalence of behavioral and emotional disorders were 40.35%, in which Behavioral disorder was 26.9%, Emotional disorder was 10.2% and both Behavioral and Emotional disorder were 3.2%. Higher length of stay and low level of education of foster mother were significantly associated with psychiatric morbidity of the respondents. Conclusions: It can be concluded that behavioral and emotional disorders are highly prevalent among orphan children and adolescents with residential care that needs to be addressed. Moreover, measure for early identification and intervention will improve the quality of life of the orphan population.DOI: http://dx.doi.org/10.3329/bsmmuj.v5i1.10997 BSMMU J 2012; 5(1):29-34
Evaluation of the Results of Posterior Decompression, Corpectomy and Instrumentation in Traumatic Unstable Thoraco-Lumbar Burst Fractures
Authors: Islam Md. Anowarul , Sakeb Najmus- , Mahmood Ehsan
Published: June 2012

Background: Thoraco-lumbar burst fractures occur as a result of axial load which often causes displacement of the middle column into the vertebral canal. Posterior surgery reduces the morbid outcomes of different other approaches. Objective: To evaluate the clinical and radiological success of posterior corpectomy and instrumentation in the management of traumatic unstable thoraco-lumbar burst fractures. Methods: It is a prospective interventional study carried out in Bangabandhu Sheikh Mujib Medical University and different private hospitals in Dhaka from July 2008 to December 2011. Total 18 patients; 13 male and 05 female within an age range of 21-40 years were selected. Total 09 cases involved L1, 05 cases at D12, 02 cases at D11 and at L2 each. Neurological status was assessed by Frankels grading and pain status by Visual Analogue Score (VAS). Paired t-test was used for statistical analysis. Results: All the patients were followed up for minimum 1 year. Eleven out of 12 patients with Frankel grade-B and 04 patients out of 06 with Frankel grade-C recovered fully and could walk without support (p<0.05). Overall 03 patients ended with some degrees of persistant neurological deficit. The mean postoperative pain improvement and kyphotic angle correction was significant  (p<0.05). Conclusion: Decompression through posterior approach by laminectomy, corpectomy and fusion by cage with bone graft and stabilization by pedicle screw and rod significantly improves the clinical and radiological outcome in management of traumatic unstable thoraco-lumbar burst fractures.DOI: http://dx.doi.org/10.3329/bsmmuj.v5i1.10998 BSMMU J 2012; 5(1):35-41
Leishmanial Hepatitis with Chronic Hepatitis B Infection Treated Successfully with Liquid Form of Liposomal Amphotericin B - A Case Report
Authors: Salma Umma , Khan Abed Hussain , Rahaman Mohammad Ferdous Ur , Irshadullah Naseeb Muhammad , Shumy Farzana , Chowdhury M A Jalil
Published: June 2012

Hepatic involvement either due to leishmaniasis or due to coexisting viral infection sometime poses a problem for the clinicians. Atypical presentation is also challenging for them. We present a case of visceral leishmaniasis (Kala-azar) from a non endemic zone, co infection with hepatitis B virus simulating chronic viral hepatitis successfully treated with liquid form of liposomal amphotericin B.DOI: http://dx.doi.org/10.3329/bsmmuj.v5i1.11018 BSMMU J 2012; 5(1):55-56
Multiple Epiphyseal Dysplasia (MED): A Rare Type of Skeletal Dysplasia
Authors: Islam Mohammad Imnul , Begum Suraiya , Talukdar Manik Kumar , Karim Md Enayet , Rahman Shahana Akhter
Published: June 2012

Multiple epiphyseal dysplasia (MED) is a congenital disorder of skeletal development that primarily affects the ends of long bones, causing progressive joint and bone inflammation and short stature. Mutations in several genes are responsible for pathogenesis of this disease. We are reporting a case of MED who presented with the complaints of multiple swelling of the joints which was associated with pain during movement for last seven years. The patient had flexion deformity of all the affected joints along with restriction of movement. These were associated with kyphosis, pectus carnitum, knock-knee and short stature. Radiological findings were suggestive of MED. Counseling was done with the parents regarding the etiology, progression and outcome of the disease.DOI: http://dx.doi.org/10.3329/bsmmuj.v5i1.11025 BSMMU J 2012; 5(1):57-60