bluuebunny/crossref_metadata_2025 huggingface dataset:
A novel assay based on fluorescence microscopy and image processing for determining phenotypic distributions of rod‐shaped bacteria
Authors: Spetsieris Konstantinos , Zygourakis Kyriacos , Mantzaris Nikos V.
Published: July 2008

Cell population balance (CPB) models can account for the phenotypic heterogeneity that characterizes isogenic cell populations. To utilize the predictive power of these models, however, we must determine the single‐cell reaction and division rates as well as the partition probability density function of the cell population. These functions can be obtained through the Collins–Richmond inverse CPB modeling methodology, if we know the phenotypic distributions of (a) the overall cell population, (b) the dividing cell subpopulation, and (c) the newborn cell subpopulation. This study presents the development of a novel assay that combines fluorescence microscopy and image processing to determine these distributions. The method is generally applicable to rod‐shaped cells dividing through the formation of a characteristic constriction. Morphological criteria were developed for the automatic identification of dividing cells and validated through direct comparison with manually obtained measurements. The newborn cell subpopulation was obtained from the corresponding dividing cell subpopulation by collecting information from the two compartments separated by the constriction. The method was applied to E. coli cells carrying the genetic toggle network with a green fluorescent marker. Our measurements for the overall cell population were in excellent agreement with the distributions obtained via flow cytometry. The new assay constitutes a powerful tool that can be used in conjunction with inverse CPB modeling to rigorously quantify single‐cell behavior from data collected from highly heterogeneous cell populations. Biotechnol. Bioeng. 2009;102: 598–615. © 2008 Wiley Periodicals, Inc.
Simultaneous determination of udenafil and its active metabolite, DA‐8164, in human plasma and urine using ultra‐performance liquid chromatography–tandem mass spectrometry: application to a pharmacokinetic study
Authors: Bae Soo Kyung , Kang Min Jeong , Yeo Chang‐Woo , Kim Min‐Jung , Shon Ji‐Hong , Liu Kwang‐Hyeon , Shin Jae‐Gook
Published: July 2008

A rapid, sensitive, and simple ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC/MS/MS) method for the determination of udenafil and its active metabolite, DA‐8164, in human plasma and urine using sildenafil as an internal standard (IS) was developed and validated. Udenafil, DA‐8164 and IS from a 100 µL aliquot of biological samples were extracted by protein precipitation using acetonitrile. Chromatographic separation was carried on an Acquity UPLC BEH C18 column (50 × 2.1 mm, i.d., 1.7 µm) with an isocratic mobile phase consisting of acetonitrile and containing 0.1% formic acid (75:25, v/v) at flow rate of 0.4 mL/min, and total run time was within 1 min. Detection and quantification was performed by the mass spectrometer using multiple reaction‐monitoring mode at m/z 517 → 283 for udenafil, m/z 406 → 364 for DA‐8164 and m/z 475 → 100 for IS. The assay was linear over a concentration range of 1–600 ng/mL with a lower limit of quantification of 1 ng/mL in both human plasma and urine. The coefficient of variation of this assay precision was less than 13.7%, and the accuracy exceeded 92.0%. This method was successfully applied for pharmacokinetic study after oral administration of udenafil 100 mg to healthy Korean male volunteers. Copyright © 2008 John Wiley & Sons, Ltd.
Separation and structure determination of nicardipine photoproducts by LC‐ESI‐MS
Authors: Chen Shih‐Ming , Hsieh Mau‐Chih , Chao Su‐Hui , Chang E. E. , Wang Po‐Yu , Wu An‐Bang
Published: July 2008

A sample of 0.104 m nicardipine in methanol was photoirradiated with a Philips 400 W UV lamp for 3 h in a photochemical chamber. A total of four major photoproducts were found from the HPLC chromatogram. The same sample was used for taking LC‐MS, while eight major photoproducts were observed and the structures elucidated by analyzing the CID patterns of their respective mass spectra. A reaction scheme of nicardipine is proposed that the photochemical reactions occur mainly via oxidation of 1,4‐dihydropyridine moiety, following the stepwise photo‐reduction of the m‐nitro group and demethylation of the ester group at 5‐position of the pyridine ring. Copyright © 2008 John Wiley & Sons, Ltd.
Pharmacokinetic study of three active flavonoid glycosides in rat after intravenous administration of Trollius ledebourii extract by liquid chromatography
Authors: Li Deqiang , Wang Qiao , Xu Lei , Li Min , Jing Xiujuan , Zhang Langtong
Published: July 2008

A selective and sensitive reversed‐phase high‐performance liquid chromatography method was developed and validated for the simultaneous determination of orientin‐2″‐O‐β‐l‐galactopyranosyl (OGA), orientin and vitexin in rat plasma. Blood samples were collected via the fossa orbitalis vein at time intervals after intravenous administration and the concentrations of the three ingredients in plasma were analyzed by HPLC after the plasma protein had been precipitated directly with methanol. OGA, orientin and vitexin were successfully separated using a C18 column with gradient elution composed of acetonitrile and 0.1% acetic acid and were detected at the detection wavelength of 348 nm. Calibration curves of OGA, orientin and vitexin were generated over the range 0.315–161, 0.326–167 and 0.215–110 µg/mL, respectively. The intra‐ and inter‐day precisions (relative standard deviation) for the analysis of the three analytes were between 1.68 and 8.43% with accuracies (relative error) below 8.55%. The mean extraction recoveries were between 70.35 and 86.42%. The developed method was suitable for simultaneous determination of these three active flavonoid glycosides in rat plasma and was successfully applied to investigate the pharmacokinetics of glycosides from Trollius ledebourii in rats. Copyright © 2008 John Wiley & Sons, Ltd.
Determination of brazilein in rat plasma after intravenous administration by HPLC
Authors: Lan Jiaqi , Hu Jun , Li Bonan , Xing Dongming , Liu Chengcheng , Wang Wei , Du Lijun
Published: July 2008

Quantification of brazilein in rat plasma following intravenous administration was achieved by reversed‐phase high‐performance liquid chromatography using a mobile phase of acetonitrile–0.05 m potassium dihydrogen phosphate water (containing 0.5% triethylamine, pH 3.0; 20:80 v/v) and UV detection at 445 nm. The method was linear (determination coefficient, r2 = 0.9992) within the tested range (0.313–5.0 µg/mL). Intra‐ and inter‐day precision coefficients of variation and accuracy bias were acceptable (maximal CV value was 2.06% for intra‐day and 1.71% for inter‐day) over the entire range. The recoveries were 81.48, 84.61 and 82.83% for concentrations of 0.313, 1.25 and 5.0 µg/mL, respectively. The concentration–time curve of brazilein after intravenous administration was fitted to the two‐compartment model. This is the first time that brazilein in rat plasma was detected by HPLC‐UV method and its pharmacokinetic characteristic was comprehensively studied. Copyright © 2008 John Wiley & Sons, Ltd.
Direct enantiomeric TLC resolution of dl‐penicillamine using (R)‐mandelic acid and l‐tartaric acid as chiral impregnating reagents and as chiral mobile phase additive
Authors: Bhushan Ravi , Agarwal Charu
Published: July 2008

dl‐Penicillamine has been resolved into its enantiomers by normal‐phase TLC using l‐tartaric acid as chiral impregnating reagent as well as chiral mobile phase additive, while (R)‐mandelic acid has been found to be successful as a chiral impregnating reagent. The solvent system acetonitrile–methanol–water (5:1:1, v/v) was found to be successful when l‐tartaric acid was used as impregnating agent while the solvent combination acetonitrile–methanol–(0.5% l‐tartaric acid in water, pH 5)–glacial acetic acid (7:1:1.1:0.7, v/v) was successful as mobile phase as it contained l‐tartaric acid as the chiral additive. (R)‐mandelic acid was successful as chiral impregnating reagent with ethyl acetate–methanol–water (3:1:1, v/v), as the mobile phase. The effects of concentration of chiral selectors, temperature and pH were examined on enantiomeric resolution. The spots were detected with iodine vapors and the detection limits were found to be 0.12 µg for each enantiomer of penicillamine with l‐tartaric acid, under both the conditions, and 0.11 µg with (R)‐mandelic acid. Copyright © 2008 John Wiley & Sons, Ltd.
Mixed micellar liquid chromatography methods: modelling quantitative retention–activity relationships of angiotensin converting enzyme inhibitors
Authors: Wu Li‐ping , Cui Yan , Xiong Mei‐jin , Wang Shu‐rong , Chen Cong , Ye Li‐ming
Published: July 2008

The capability of biopartitioning micellar chromatography (BMC), using pure Brij35 solution and mixed micellar system of Brij35–SDS (85:15) as mobile phase, to describe and estimate bioactivities of angiotensin converting enzyme inhibitors at different pH has been studied. Quantitative retention–activity relationships (QRAR) in BMC were investigated for these compounds. The obtained BMCBrij35–SDS–QRAR models were compared with the traditional BMCBrij35–QRAR, and better statistically models were obtained using Brij35‐SDS retention data. The superiority of BMCBrij35–SDS–QRAR is due to the fact that the mixed micellar mobile phase can simulate the resting membrane potential and the conformation of the long hydrophilic polyoxyethylene chains remains unchanged. Copyright © 2008 John Wiley & Sons, Ltd.
High‐performance liquid chromatography and LC‐ESI‐MS method for the identification and quantification of two biologically active isomeric coumarinolignoids cleomiscosin A and cleomiscosin B in different extracts of Cleome viscosa
Authors: Chattopadhyay Sunil K. , Kumar Satyanshu , Tripathi Sarita , Kaur Ranjeet , Tandon Sudeep , Rane Shailendra
Published: July 2008

A rapid, sensitive and simple reverse‐phase high‐performance liquid chromatographic–electrospray ionization–mass spectrometry method for simultaneous determination of cleomiscosin A and cleomiscosin B has been developed and validated. The isomeric coumarinolignoids cleomiscosin A (1) and cleomiscosin B (2) were separated on a Waters symmetry C18 column with a solvent system composed of acetonitrile–methanol (1:2) and acetic acid–water (0.5 : 99.5) in a gradient elution mode. The absorption at 326 nm was chosen as the measuring wavelength in which resolution and baseline separation of compounds 1 and 2 could be obtained. The identity of the two isomeric compounds 1 and 2 in the samples were determined on a triple quadrupole mass spectrometer with ESI interface operating in the positive mode. Calibration curves were linear (r2 > 0.993) over the concentration range 20–200 µg/mL for cleomiscosin A and 10–200 µg/mL for cleomiscosin B with acceptable accuracy and precision, respectively. The intra‐day and inter‐day precision were 1.13 and 0.82% for cleomiscosin A and 1.78 and 1.28% for cleomiscosin B, respectively. The validated method was successfully applied for the analysis of the above two compounds in different extracts of Cleome viscosa. Copyright © 2008 John Wiley & Sons, Ltd.
Subunit structure of glycinin and its molecular species based on RP‐HPLC, gel electrophoresis and SEC studies
Authors: Bhushan Ravi , Arya Upasana
Published: July 2008

Glycinin and its molecular species (glycinin I and glycinin II) were separated and isolated. The number and kind of subunits of glycinin, glycinin I and glycinin II were determined. Studies were carried out under different experimental conditions using slab gel electrophoresis, size‐exclusion chromatography and reversed‐phase high performance liquid chromatography. Gel electrophoresis was done using both continuous and discontinuous system and under varying concentrations of resolving gel. In addition, the subunits were separated by reversed phase using gradient program. Glycinin and glycinin II were found to have 12 subunits each while glycinin I showed six subunits. Molecular weight and weight ratio in each case were also determined. Copyright © 2008 John Wiley & Sons, Ltd.
Rapid and sensitive HPLC‐MS/MS method for pharmacokinetic assessment of ribavirin in healthy Chinese
Authors: Zhou Zhi‐Ling , Yu Xi‐Yong , Yang Min , Peng Huai‐Yan , Chen Tie‐Feng , Lin Qiu‐Xiong , Shan Zhi‐Xin , Liu Xiao‐Ying , Deng Chun‐Yu , Zhu Ping , Huang Xiao‐Zhong , Lin Shu‐Guang
Published: July 2008

A rapid and sensitive quantitative assay method was developed for determining ribavirin pharmacokinetic in human plasma. The chromatographic separation was achieved within 4.5 min using a SinoChrom ODS‐BP column (4.6 × 150 mm, 5 µm) with acetonitrile–water (1 mmol/L ammonium acetate buffer, 0.1% formic acid; 15:85, v/v) at a constant flow rate of 0.8 mL/min. The MRM pairs were m/z 245.2 → m/z 113.1 for ribavirin and m/z 226.1 → m/z 152.1 for acyclovir (internal standard), respectively, with dwell times of 200 ms for each transition. The results showed calibration curve for ribavirin was linear over a concentration range of 1–1000 ng/mL. The lower limit of quantification (LLOQ) was 1 ng/mL ribavirin. Twenty healthy volunteers received a 300 mg oral dose of ribavirin. Blood samples were then collected up to 120 h postdosing. All plasma data were comodeled for ribavirin by using noncompartmental modeling. The single dose of ribavirin was well tolerated and no serious adverse effects occurred. The mean time to maximum concentration was about 1.25 h. The mean maximum concentration of drug in plasma for oral ribavirin was 250 ng/mL. The mean elimination half‐life was 43.6 h. The present study describes a simple, specific, sensitive HPLC–MS/MS method for measuring plasma drug concentration and analyzing human pharmacokinetics of ribavirin. Copyright © 2008 John Wiley & Sons, Ltd.
Synthetic Antimicrobial Peptides as Agricultural Pesticides for Plant‐Disease Control
Authors: Montesinos Emilio , Bardají Eduard
Published: July 2008

There is a need of antimicrobial compounds in agriculture for plant‐disease control, with low toxicity and reduced negative environmental impact. Antimicrobial peptides are produced by living organisms and offer strong possibilities in agriculture because new compounds can be developed based on natural structures with improved properties of activity, specificity, biodegradability, and toxicity. Design of new molecules has been achieved using combinatorial‐chemistry procedures coupled to high‐throughput screening systems and data processing with design‐of‐experiments (DOE) methodology to obtain QSAR equation models and optimized compounds. Upon selection of best candidates with low cytotoxicity and moderate stability to protease digestion, anti‐infective activity has been evaluated in plant–pathogen model systems. Suitable compounds have been submitted to acute toxicity testing in higher organisms and exhibited a low toxicity profile in a mouse model. Large‐scale production can be achieved by solution organic or chemoenzymatic procedures in the case of very small peptides, but, in many cases, production can be performed by biotechnological methods using genetically modified microorganisms (fermentation) or transgenic crops (plant biofactories).
Synthesis and Biological Evaluation of Novel Pyrazolo[4,3‐b]oleanane Derivatives as Inhibitors of Glycogen Phosphorylase
Authors: Chen Jun , Gong Yanchun , Liu Jun , Hua Weiyi , Zhang Luyong , Sun Hongbin
Published: July 2008

Eighteen pyrazolo[4,3‐b]oleanane derivatives have been synthesized and biologically evaluated as inhibitors of rabbit muscle GPa. Key compound 5 was readily obtained in four steps starting from oleanolic acid (OA; 1). Further modification based on pyrazolo triterpene 5 resulted in 17 novel pyrazolo pentacyclic triterpenes. All of the synthesized pyrazolo[4,3‐b]oleanane derivatives were biologically assayed against rabbit muscle GPa. Within this series of compounds, pyrazole triterpene 19 (IC50=9.9 μM) exhibited more potent activity than the parent compound 1. Preliminary structure–activity relationship analysis of the pyrazolo[4,3‐b]oleanane derivatives as GPa inhibitors is discussed.
Evaluation of Organophosphorus Chemicals‐Degrading Enzymes: A Comparison of Escherichia coli and Human Cytosolic Aminopeptidase P
Authors: Hsu Yu‐Ting , Su Chiao‐Yung , Du Huang‐Chi , Jao Shu‐Chuan , Li Wen‐Shan
Published: July 2008

An enzyme capable of hydrolyzing organophosphate compounds is of biological as well as environmental significance. We evaluated the possibility of human cytosolic aminopeptidase P (hcAMPP) as an attractive bioscavenger candidate by measuring the enzymatic rates of hydrolysis for a wide variety of organophosphorus compounds. The comparison of substrate specificity exhibited by hcAMPP and E. coli aminopeptidase P (E. coli AMPP) was studied.

We cloned, expressed, and purified hcAMPP from HeLa cells and AMPP from Escherichia coli. The pH‐rate profiles of hcAMPP were measured in the presence of organophosphate compound 3 or 5. All of the organophosphorus compounds, 1–19, were synthesized by using the approach of phosphorus chemistry described in a previous publication. The relative activity of hcAMPP and E. coli AMPP in hydrolyzing a series of organophosphorus analogues, 1–17, was evaluated in a spectrophotometric assay by monitoring the difference of accumulation of 4‐nitrophenol at 400 nm.

The overall substrate preference of hcAMPP is as follows: methylphosphonates>ethylphosphonates≥organophosphates. Interestingly, the observed enhancement in the activity of hcAMPP with methyl phosphonates, 8, 10, 12, and 13, suggests that there is particularly special about the substructure of both methyl moiety and PO ligand, since the values of specific activity with hcAMPP for the methylphosphonates 8, 10, 12, and 13 are 2‐ to 73‐fold greater than those for the ethylphosphonates 14–17 and the organophosphates 1–7. Similarly, in E. coli AMPP toward ethylphosphonates 14–17, the results indicate that the regions of both MeO moiety and PO ligand may be located in the vicinity of the substrate‐binding site, which have not been altered within the active site of enzyme upon mutation of Trp88, Arg153, and Arg370.

These studies demonstrate that E. coli AMPP and hcAMPP display different substrate preference toward organophosphorus compounds. Evidence here, therefore, represents the first example of hcAMPP that might serve as a valuable bioscavenger candidate as E. coli AMPP due to the promise from the hydrolysis of these toxic chemicals.
Argusides D and E, Two New Cytotoxic Triterpene Glycosides from the Sea Cucumber Bohadschia argusJaeger
Authors: Liu Bao‐Shu , Yi Yang‐Hua , Li Ling , Sun Peng , Han Hua , Sun Guo‐Quan , Wang Xiao‐Hua , Wang Zeng‐Lei
Published: July 2008

Two new triterpene glycosides, argusides D and E (1 and 2, resp.), have been isolated from the sea cucumber Bohadschia argus Jaeger collected in the South China Sea. Their structures have been established by spectral analysis (ESI‐MS, and 1D‐ and 2D‐NMR) and chemical evidence. Compounds 1 and 2 both possess an holostane‐type triterpene aglycone with a C(9)C(11) bond, an OH group at C(12), and tetrasaccharide moieties, but differ in the side chains. The two glycosides exhibited significant cytotoxicities against four human tumor cell lines, A549, HCT‐116, HepG2, and MCF‐7.
Distal myocardial protection with intracoronary beta blocker when added to a Gp IIb/IIIa platelet receptor blocker during percutaneous coronary intervention improves clinical outcome
Authors: Uretsky Barry F. , Birnbaum Yochai , Osman Abdulfatah , Gupta Rajiv , Paniagua Oscar , Chamoun Antonio , Pohwani Amir , Lui Charles , Lev Eli , McGehee Todd , Kumar Darren , Akhtar Asif , Anzuini Angelo , Schwarz Ernst R. , Wang Fen Wei
Published: July 2008

Objective: The present study tested the hypothesis that intracoronary (IC) propranolol improves clinical outcomes with percutaneous coronary intervention (PCI) when used with background Gp IIb/IIIa receptor blockade. Background: We have previously shown that administration of a relatively large weight‐based IC dose of the beta blocker propranolol before PCI decreases the incidence of post‐PCI myocardial infarction (MI) and improves short‐ and long‐term outcome. It has previously been shown that administration of a Gp IIb/IIIa receptor blocker decreases post‐PCI MI and improves short‐ and long‐term clinical outcome. Methods: Patients undergoing PCI (n = 400) were randomized in a prospective double‐blind fashion to IC propranolol (n = 200) or placebo (n = 200) with eptifibatide administered to all the patients. Myocardial isoform of creatine kinase was measured during the first 24 hr and clinical outcomes at 30 days and 1 year. Results: MI after PCI was seen in 21.5% of placebo and 12.5% of propranolol patients (relative risk reduction 0.42; 95%CI 0.09, 0.63; P = 0.016). At 30 days, the composite end point of death, post‐procedural MI, urgent target lesion revascularization, or MI after index hospitalization occurred in 22.5% of placebo vs. 13.5% of propranolol patients (risk reduction 0.43; 95%CI 0.08, 0.65; P = 0.018). Similar results were observed at 1 year with adverse outcomes in 21.5% of propranolol and 32.5% of placebo patients (P = 0.01). Conclusion: IC propranolol administration with the background Gp IIb/IIIa receptor blockade significantly reduces the incidence of post‐PCI MI and improves the short‐ and long‐term clinical outcome when compared with a Gp IIb/IIIa blocker alone. © 2008 Wiley‐Liss, Inc.
Transradial versus transfemoral approach for coronary angiography and intervention in patients above 75 years of age
Authors: Achenbach Stephan , Ropers Dieter , Kallert Lisa , Turan Nesrin , Krähner Robert , Wolf Tobias , Garlichs Christoph , Flachskampf Frank , Daniel Werner G. , Ludwig Josef
Published: July 2008

Aims: The transradial (TR) approach has potentially lower complication rates than transfemoral (TF) approach coronary angiography. However, it may be technically more challenging, especially in elderly patients with alterations in vascular anatomy. We thus determined success rates, procedural data, and complication rates of TR angiography in comparison to the TF approach in elderly patients in a randomized, prospective trial. Methods and results: Four hundred consecutive patients ≥75 years with known or suspected coronary artery disease were included in the study. After exclusion of 93 patients with contraindications to the radial approach, 152 patients were randomized to the TR and 155 to TF coronary angiography and intervention. In 13 patients randomized to TR, cross‐over to TF was necessary (9%). Total examination time was significantly longer for the TR approach (18.1 vs. 15.0 min, P = 0.009), but no difference was found for fluoroscopy time, number of catheters used, or amount of contrast agent. The rate of major complications (bleeding requiring surgery or transfusion, stroke) was 0% for TR and 3.2% for TF approach (P < 0.001). Minor complications occurred in 1.3% versus 5.8% of patients (P < 0.001). Conclusion: In elderly patients, TR coronary angiography and intervention has a high technical success rate and lower complication rates than the TF approach. © 2008 Wiley‐Liss, Inc.
The off‐ versus on‐label use of medical devices in interventional cardiovascular medicine: Clarifying the ambiguity between regulatory labeling and clinical decision‐making, Part 1: PCI
Authors: Price Matthew J. , Teirstein Paul S.
Published: July 2008

The Food and Drug Administration convened a special public meeting of the Circulatory System Devices Advisory Panel in late 2006 in response to data suggesting a small but potentially significant increased risk of stent thrombosis with drug‐eluting stents (DES). This panel concluded that “off‐label” DES use was associated with an increased risk of adverse outcomes compared with “on‐label” use. In this commentary, we will discuss the role of product labeling in clinical decision‐making during percutaneous coronary intervention, elucidate the issues that may arise from the conflation of the responsibilities of regulatory bodies and physicians, and offer a potential framework for their resolution. © 2008 Wiley‐Liss, Inc.
Left ventricular unloading with intra‐aortic counter pulsation prior to reperfusion reduces myocardial release of endothelin‐1 and decreases infarction size in a porcine ischemia‐reperfusion model
Authors: LeDoux John F. , Tamareille Sophie , Felli Patty R. , Amirian James , Smalling Richard W.
Published: July 2008

Objectives:

We tested the hypothesis that unloading the left ventricle with intra‐aortic balloon counter‐pulsation just prior to reperfusion provides infarct salvage compared with left ventricular (LV) unloading postreperfusion or reperfusion alone.

Background:

Previous reports demonstrated infarct salvage with complete LV unloading with an LVAD prior to reperfusion; however, partial LV unloading using intra‐aortic balloon pumps (IABPs) has not been evaluated.

Methods:

Twenty‐eight Yorkshire pigs were subjected to 1 hr of left anterior descending artery occlusion and 4 hr of reperfusion. An IABP was inserted and activated just prior to reperfusion (IABP‐Pre), or 15 min after reperfusion (IABP‐Post), or not at all (control).

Results:

At baseline, the hemodynamic data were similar in the three groups. Myocardial infarct size expressed a percentage of zone at risk in control animals was 44.9% ± 4.8%, IAPB‐Pre group 20.9% ± 5.1% (P < 0.05 compared to control), and IABP‐Post group 33.2 ± 6.1% (P = 0.16 vs. control group). There was a correlation between transcardiac endothelin‐1 release at 15 min postreperfusion and infarct size (r = 0.59).

Conclusion:

LV unloading with an IABP prior to reperfusion reduces the extent of myocardial necrosis in hearts subjected to 1 hr of left anterior descending artery occlusion and 4 hr of reperfusion compared with either reperfusion alone or LV unloading after reperfusion. Inhibition of myocardial ET‐1 release by LV unloading may be a significant mechanism of myocardial protection. These data suggest that in high‐risk STEMI patients, IABP unloading prior to reperfusion might be more beneficial than IABP placement postreperfusion. © 2008 Wiley‐Liss, Inc.
Comparison of sirolimus‐eluting stent and paclitaxel‐eluting stent for long‐term cardiac adverse events in diabetic patients: The Korean multicenter angioplasty team (KOMATE) registry
Authors: Kim Jung‐Sun , Lee Byoung Ho , Ko Young‐Guk , Choi Donghoon , Jang Yangsoo , Min Pil‐Ki , Yoon Young‐Won , Hong Bum Kee , Kwon Hyuck Moon , Ahn Min‐Soo , Lee Seung‐Hwan , Yoon Jung Han , Lee Byoung Kwon , Kim Byung Ok , Kim Byeong‐Kuk , Oh Sung Jin , Jeon Dong Woon , Yang Joo Young , Cho Jung Rae , Jung Jae‐Hun , Ryu Seung‐Ki
Published: July 2008

Background: There is some controversy on long‐term cardiac outcomes between sirolimus‐eluting stents (SES) and paclitaxel‐eluting stents (PES) in diabetes mellitus (DM). We compared cardiac adverse events after SES and PES implantation in patients with DM over a period of 3 year. Methods: A total of 634 patients with DM treated with SES (n = 428) or PES (n = 206) were consecutively enrolled in the KOMATE registry from 2003 to 2004. We assessed major adverse cardiac events (MACEs, cardiovascular death, nonfatal myocardial infarction, ischemia driven target vessel revascularization) and stent thrombosis (ST) according to the definitions set by the Academic Research Consortium. Results: Propensity score (PS) analysis was performed to adjust different baseline characteristics. The mean follow‐up duration was 38 ± 8 month (at least 36 month and up to 53 month). The 3‐year MACE rate did not show a significant difference between the two groups [52 (12.1%) in SES vs. 29 (14.1%) in PES, P = 0.496]. The definite and probable ST at 3 year were similar in both SES and PES [12 (2.8%) in SES vs. 7 (3.4%) in PES, P = 0.681]. There were no differences in hazard ratio for MACE and ST between two stents [MACE, crude: 0.844 (0.536–1.330) and adjusted for PS: 0.858 (0.530–1.389); ST, crude: 0.820 (0.323–2.083) and adjusted for PS: 0.960 (0.357–2.587)]. Conclusions: The present study demonstrated that long‐tem cardiac outcomes including ST were not significantly different between SES and PES in patients with DM. © 2008 Wiley‐Liss, Inc.
Feasibility and efficacy of stent redilatation in aortic coarctation
Authors: Zanjani Keyhan S. , Sabi Titus , Moysich Axel , Ovroutski Stanislav , Peters Bjoern , Miera Oliver , Kühne Titus , Nagdyman Nicole , Berger Felix , Ewert Peter
Published: July 2008

Objectives: We evaluated the efficacy and safety of stent redilation in patients with aortic coarctation (COA). Methods and Results: In our retrospective study of 28 stent redilation procedures on 22 patients, the interval between implantation and redilation ranged from 2.5 month to 11 years (median 18 months). Ten patients had native COAs, one had repaired interrupted aortic arch. The remaining patients had residual COAs after surgery. Stent redilation was successful in 26 procedures. Pressure gradients decreased from a mean of 26 ± 13.8 mm Hg to 7.9 ± 6.52 mm Hg (P < 0.001). The mean diameter of the stents increased from 9.19 ± 3.29 mm to 12.1 ± 3.68 mm (P < 0.001). In a patient with Williams‐Beuren syndrome, massive neointimal proliferation made stent redilation impossible. One other patient developed aortic aneurysm after redilation and was operated after 3 years. Conclusions: Stent redilation for aortic COA is a safe and successful procedure. © 2008 Wiley‐Liss, Inc.
The off‐versus on‐label use of medical devices in interventional cardiovascular medicine?: Clarifying the ambiguity between regulatory labeling and clinical decision making, Part III: Structural heart disease interventions
Authors: Holzer Ralf , Hijazi Ziyad
Published: July 2008

The majority of devices used to treat congenital cardiac lesions in the cardiac catheterization laboratory are used on an off‐label basis. This article discusses the predicaments faced by the interventional cardiologist when implanting devices on an off‐label basis, using procedural examples of PFO closure and endovascular stent placement. Strategies that may benefit patient and provider, as well as satisfying regulatory agencies are suggested. © 2008 Wiley‐Liss, Inc.
Comparison between ticlopidine and clopidogrel in patients undergoing primary stenting in acute myocardial infarction: Results from the CADILLAC trial
Authors: Lansky Alexandra J. , Tsuchiya Yoshihiro , Brener Micheal , Mehran Roxana , Cristea Ecaterina , Pietras Cody , Grines Cindy L. , Cox David A. , Garcia Eulogio , Tcheng James E. , Guagliumi Giulio , Stuckey Thomas , Turco Mark , Carroll John D. , Rutherford Barry D. , Leon Martin B. , Moses Jeffrey , Stone Gregg W.
Published: July 2008

Objective:

The aim of this article is to examine whether clopidogrel and ticlopidine treatments produce similar clinical outcomes for patients receiving primary stenting for acute myocardial infarction (AMI).

Background:

Prior studies have yielded conflicting results on the relative safety and efficacy of clopidogrel and ticlopidine after stent implantation, warranting an evaluation in primary stenting for AMI.

Methods:

In the multicenter, prospective CADILLAC trial, patients undergoing primary infarct stenting were treated at operator discretion with either ticlopidine (931 patients) or clopidogrel (163 patients) and then followed for 1 year. Baseline clinical and angiographic characteristics were comparable except for baseline TIMI 0/1 flow (72.5% clopidogrel vs. 63.9% ticlopidine, P = 0.04).

Results:

Patients receiving clopidogrel had more recurrent ischemia in hospital (6.1 vs. 2.8%, P = 0.02) and at 30 days (10.5 vs. 5.8%, P = 0.02), more moderate and severe bleeding at 30 days (7.4 vs. 2.7%, P = 0.002), and similar rates of stent thrombosis out to 1 year (P = 0.11). By multivariable analysis, clopidogrel use was an independent predictor for recurrent ischemia in hospital (P = 0.0002), and at 30 days (P = 0.012); and of moderate and severe bleeding in hospital (P = 0.002), and at 30 days (P = 0.001).

Conclusions:

Despite thienopyridines similarities, their efficacy may be different within the first 30 days of primary stenting for AMI. A prospective, randomized trial is required to confirm these findings. © 2008 Wiley‐Liss, Inc.
ChemInform Abstract: Equilibrium Structure and Torsional Barrier of BH3NH3.
Authors: Demaison Jean , Lievin Jacques , Csaszar Attila G. , Gutle Claudine
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Superconductivity at 43 K in an Iron‐Based Layered Compound LaO1‐xFxFeAs.
Authors: Takahashi Hiroki , Igawa Kazumi , Arii Kazunobu , Kamihara Yoichi , Hirano Masahiro , Hosono Hideo
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Small Neutral Molecule with Two Noble‐Gas Atoms: HXeOXeH.
Authors: Khriachtchev Leonid , Isokoski Karoliina , Cohen Arik , Raesaenen Markku , Gerber R. Benny
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: YF[MoO4] and YCl[MoO4]: Two Halide Derivatives of Yttrium ortho‐Oxomolybdate: Syntheses, Structures, and Luminescence Properties.
Authors: Schleid Thomas , Strobel Sabine , Dorhout Peter K. , Nockemann Peter , Binnemans Koen , Hartenbach Ingo
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Solution Dynamics and Gas‐Phase Chemistry of Pd2@Sn4‐18.
Authors: Kocak F. Sanem , Zavalij Peter , Lam Yiu‐Fai , Eichhorn Bryan W.
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of Methyl‐α‐D‐glucopyranoside‐Based Azacrown Ethers and Their Application in Enantioselective Reactions.
Authors: Mako Attila , Szoelloesy Aron , Keglevich Gyoergy , Menyhard Dora K. , Bako Peter , Toeke Laszlo
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Direct Substitution of the Hydroxy Group at the Allylic/Propargylic Position with Carbon‐ and Heteroatom‐Centered Nucleophiles Catalyzed by Yb(OTf)3.
Authors: Huang Wen , Shen Quan‐Sheng , Wang Jia‐Liang , Zhou Xi‐Geng
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: [8 + 2] and [8 + 3] Cyclization Reactions of Alkenyl Carbenes and 8‐Azaheptafulvenes: Direct Access to Tetrahydro‐1‐azaazulene and Cyclohepta[b]pyridinone Derivatives.
Authors: Barluenga Jose , Garcia‐Rodriguez Jaime , Martinez Silvia , Suarez‐Sobrino Angel L. , Tomas Miguel
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis, Antitubercular and Antimicrobial Activities of Some New Pyrazoline and Isoxazole Derivatives.
Authors: Vyas D. H. , Tala S. D. , Dhaduk M. F. , Akbari J. D. , Joshi H. S.
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis and QSAR Studies of Thiosemicarbazides, 1,2,4‐Triazoles, 1,3,4‐Thiadiazoles and 1,3,4‐Oxadiazoles Derivatives as Potential Antibacterial Agents.
Authors: Desai N. C. , Bhavsar A. M. , Shah M. D. , Saxena Anil K.
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Selective Oxidation of Organic Compounds Using Pyridinium‐1‐sulfonate Fluorochromate, C5H5NSO3H [CrO3F] (PSFC).
Authors: Bekhradnia Ahmad R. , Zahir Forohe , Arshadi Sattar
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Facile and Convenient Method for the Conversion of Thioamides into Amides Using Pyridinium Hydrobromide Perbromide.
Authors: Lakouraj Moslem M. , Ghodrati Keivan
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: gem‐Difluorination of Aminoalkynes via Highly Reactive Dicationic Species in Superacid HF—SbF5: Application to the Efficient Synthesis of Difluorinated Cinchona Alkaloid Derivatives.
Authors: Cantet Anne‐Celine , Carreyre Helene , Gesson Jean‐Pierre , Jouannetaud Marie‐Paule , Renoux Brigitte
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Convenient Synthesis of tert‐Butyl Ethers under Microwave Condition.
Authors: Mahammed K. A. , Murthy P. S. Keshava , Raju K. Mohana
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Rh(I)‐Catalyzed Hydration of Organonitriles under Ambient Conditions.
Authors: Goto Akihiro , Endo Kohei , Saito Susumu
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Novel Synthesis of 2‐Aminopentanedinitriles from 2‐(Bromomethyl)aziridines and Their Transformation into 2‐Imino‐5‐methoxypyrrolidines and 5‐Methoxypyrrolidin‐2‐ones.
Authors: D'hooghe Matthias , Van Nieuwenhove Andries , Van Brabandt Willem , Rottiers Mario , De Kimpe Nobert
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Short Access to Highly Strained Spiranic Compounds from Ethyl 3‐Cyclobutylprop‐2‐enoate.
Authors: Salim Hani , Piva Olivier
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Nucleophilic Fluoroalkylation of Iminium Salts.
Authors: Levin Vitalij V. , Kozlov Mikhail A. , Song Young‐Hun , Dilman Alexander D. , Belyakov Pavel A. , Struchkova Marina I. , Tartakovsky Vladimir A.
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: epi‐Cinchona‐Based Thiourea Organocatalyst Family as an Efficient Asymmetric Michael Addition Promoter: Enantioselective Conjugate Addition of Nitroalkanes to Chalcones and α,β‐Unsaturated N‐Acylpyrroles.
Authors: Vakulya Benedek , Varga Szilard , Soos Tibor
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Environmentally Benign and Selective Reduction of Nitroarenes with Fe in Pressurized CO2—H2O Medium.
Authors: Gao Ge , Tao Yuan , Jiang Jingyang
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Allylsilane—Vinylarene Cross‐Metathesis Enables a Powerful Approach to Enantioselective Imine Allylation.
Authors: Huber John D. , Perl Nicholas R. , Leighton James L.
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Facile Synthesis of N‐Sulfonyl and N‐Sulfinyl Aldimines under Barbier‐Type Conditions.
Authors: Fan Renhua , Pu Dongming , Wen Fengqi , Ye Yang , Wang Xiaoli
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Highly Diastereoselective Synthesis of Orthoquinone Monoketals Through λ3‐Iodane‐Mediated Oxidative Dearomatization of Phenols.
Authors: Pouysegu Laurent , Chassaing Stefan , Dejugnac Delphine , Lamidey Anne‐Marie , Miqueu Karinne , Sotiropoulos Jean‐Marc , Quideau Stephane
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Syntheses of (Z)‐Allyl Chlorides from Baylis—Hillman Adducts with a Trichlorotriazine/DMF System.
Authors: Li Jian , Li Shaoyu , Jian Xueshun , Zhang Yongmin
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Asymmetric Catalysis via Dynamic Substrate/Ligand/Rare Earth Metal Conglomerate.
Authors: Nojiri Akihiro , Kumagai Naoya , Shibasaki Masakatsu
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of Rhodium Colloidal Nano‐Coating Grafted Mesoporous Silica Composite and Its Application as Efficient Environmentally Benign Catalyst for Heck‐Type Reaction of Arylboronic Acids.
Authors: Li Liang , Shi Jianlin
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: TiO2: A Simple and an Efficient Catalyst for Esterification of Phenols under Solvent‐Free Condition.
Authors: Pasha Mohamed Afzal , Manjula Krishnappa
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Practical Oxidation of Sulfides to Sulfones by H2O2 Catalyzed by Titanium Catalyst.
Authors: Al‐Maksoud Walid , Daniele Stephane , Sorokin Alexander B.
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Convenient New Method to Construct 1‐Alkynyl Cyclopropanol and Its Synthetic Application to Prepare Trisubstituted Dienones.
Authors: An Yan , Liu Jie , Jiang Hai‐Ying , Wang Yahui , Chen Zili
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Highly Enantioselective Alkynylation of Aldehydes Catalyzed by a New Oxazolidine—Titanium Complex.
Authors: Xu Zhou , Mao Jincheng , Zhang Yawen
Published: July 2008

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.