bluuebunny/crossref_metadata_2025 huggingface dataset:
ШКОЛА ЦЕЛОСТНОГО РАЗВИТИЯ В СЕЛЕ ИВАНОВСКОЕ ЯРОСЛАВСКОЙ ОБЛАСТИ: ТЕОРИЯ ВОПРОСА
Authors: Мартышин Владимир Сергеевич
Published: December 2022

Статья В. С. Мартышина – известного педагога, основателя Ивановской школы целостного развития, до недавнего времени ее директора, а ныне священника церкви, выстроенной рядом со школой, раскрывает вопрос о методологических подходах, которыми руководствуется эта школа. Автор пишет о том, что опирается на русскую классическую педагогическую традицию, на подходы западноевропейских мыслителей-педагогов, но развивает собственный подход «школы целостного развития». За целостностью здесь стоит и особый набор дисциплин, охватывающих весь образовательно-воспитательный спектр, необходимый современному школьнику, и опора на изучение духовно-нравственных дисциплин – добротолюбия, родоведения и т. д., обширная краеведческая работа, погружение в исторические эпохи и т. д. Раскрываются теоретические подходы, используемые в школе для достижения поставленной цели «целостного развития».
An article by V. S. Martyshin, a well-known teacher, the founder of the Ivanovo School of Holistic Development, until recently its principal, and now the priest of the church built next to the school, reveals the question of the methodological approaches that guide this school. The author writes that he relies on the Russian classical pedagogical tradition, on the approaches of Western European thinkers and teachers, but develops his own approach of the «holistic development school». Behind the integrity there is also a special set of disciplines that cover the entire educational spectrum necessary for a modern schoolchild, and reliance on the study of spiritual and moral disciplines - benevolence, ancestry, etc., extensive local history work, immersion in historical eras, etc. The theoretical approaches used at school to achieve the goal of «holistic development» are revealed.
An induced population of Trypanosoma cruzi epimastigotes more resistant to complement lysis promotes a phenotype with greater differentiation, invasiveness, and release of extracellular vesicles
Authors: Rossi Izadora Volpato , Nunes Maria Alice Ferreira , Sabatke Bruna , Ribas Hennrique Taborda , Winnischofer Sheila Maria Brochado , Ramos Augusto Savio Peixoto , Inal Jameel Malhador , Ramirez Marcel Ivan
Published: December 2022

Introduction

Chagas disease is a neglected tropical disease caused by Trypanosoma cruzi, which uses blood-feeding triatomine bugs as a vector to finally infect mammalian hosts. Upon entering the host, the parasite needs to effectively evade the attack of the complement system and quickly invade cells to guarantee an infection. In order to accomplish this, T. cruzi expresses different molecules on its surface and releases extracellular vesicles (EVs).

Methods

Here, we have selected a population of epimastigotes (a replicative form) from T. cruzi through two rounds of exposure to normal human serum (NHS), to reach 30% survival (2R population). This 2R population was characterized in several aspects and compared to Wild type population.

Results

The 2R population had a favored metacyclogenesis compared with wild-type (WT) parasites. 2R metacyclic trypomastigotes had a two-fold increase in resistance to complementmediated lysis and were at least three times more infective to eukaryotic cells, probably due to a higher GP82 expression in the resistant population. Moreover, we have shown that EVs from resistant parasites can transfer the invasive phenotype to the WT population. In addition, we showed that the virulence phenotype of the selected population remains in the trypomastigote form derived from cell culture, which is more infective and also has a higher rate of release of trypomastigotes from infected cells.

Conclusions

Altogether, these data indicate that it is possible to select parasites after exposure to a particular stress factor and that the phenotype of epimastigotes remained in the infective stage. Importantly, EVs seem to be an important virulence fator increasing mechanism in this context of survival and persistence in the host.
Gender-specific factors associated with hypertension among women of childbearing age: Findings from a nationwide survey in India
Authors: Chhabra Pragti , Behera Shyambhavee , Sharma Rahul , Malhotra Rajeev Kumar , Mehta Kedar , Upadhyay Kritika , Goel Sonu
Published: December 2022

Background

The association of conventional (modifiable and non-modifiable) risk factors for hypertension has already been established in the literature. However, there are other putative risk factors specific to women (early menarche, age at first childbirth, women empowerment, number of children born, hysterectomy, etc.) in the development of hypertension. This study is the first study to highlight the potential association of gender-specific factors along with other conventional risk factors and hypertension, using a nationwide sample.

Methods

The study is a secondary analysis of the data collected from the National Family Health Survey-4 (NFHS-4), a nationally representative sample of 699,686 women of reproductive age in India. The interview schedule included data on general background characteristics, marriage, reproductive history, hysterectomy, knowledge, and utilization of family planning services, maternal and child care, women empowerment, non-communicable diseases, and domestic violence. The blood pressure was measured by direct observation by the study investigators using a digital blood pressure monitor. To account for disproportionate sampling and non-response, a weighted statistical analysis was performed. Logistic regression analysis was done to study the strength of the association between the risk factors and hypertension (computation of unadjusted and adjusted odds ratio).

Results

The prevalence of hypertension was 11.8% among women. Among the conventional factors, older age, higher body mass index (BMI), tobacco use, and alcohol use had higher odds for hypertension, while higher education, higher socio-economic position, and living in urban areas had lower odds. Among the gender-specific factors, younger age at first childbirth, early menarche, oral contraceptive pill use [adjusted OR: 1.23; (1.18–1.28)], and hysterectomy [adjusted OR: 1.10; (1.05–1.69)] were found to be risk factors for hypertension. Domestic violence was significantly associated with hypertension [unadjusted OR: 1.11; (1.02–1.20)]. Empowered women had lower odds of hypertension [adjusted OR: 0.93; (0.95–1.03)].

Conclusion

Significant association of these gender-specific factors among women necessitates the need for taking into account these factors while screening for hypertension among women and thus, designing a tailored model better suited to them for risk assessment.
A Mendelian randomization study to assess the genetic liability of type 1 diabetes mellitus for IgA nephropathy
Authors: Deng Peizhi , Li Zhixin , Yi Bin , Leng Yiping
Published: December 2022

Background

The prevalence of immunoglobulin A nephropathy (IgAN) seems to be higher in patients with type 1 diabetes mellitus (T1DM) than that in the general population. However, whether there exists a causal relationship between T1DM and IgAN remains unknown.

Methods

This study conducted a standard two-sample Mendelian randomization (MR) analysis to assess the causal inference by four MR methods, and the inverse variance-weighted (IVW) approach was selected as the primary method. To further test the independent causal effect of T1DM on IgAN, multivariable MR (MVMR) analysis was undertaken. Sensitivity analyses incorporating multiple complementary MR methods were applied to evaluate how strong the association was and identify potential pleiotropy.

Results

MR analyses utilized 81 single-nucleotide polymorphisms (SNPs) for T1DM. The evidence supports a significant causal relationship between T1DM and increased risk of IgAN [odds ratio (OR): 1.39, 95% confidence interval (CI): 1.10–1.74 for IVW, p < 0.05]. The association still exists after adjusting for triglyceride (TG), fasting insulin (FI), fasting blood glucose (FBG), homeostasis model assessment of beta-cell function (HOMA-B) and insulin resistance (HOMA-IR), and glycated hemoglobin (HbA1c). MVMR analysis indicated that the effect of T1DM on IgAN vanished upon accounting for low-density lipoprotein cholesterol (LDL-c; OR: 0.97, 95% CI: 0.90–1.05, p > 0.05).

Conclusions

This MR study provided evidence that T1DM may be a risk factor for the onset of IgAN, which might be driven by LDL-c. Lipid-lowering strategies targeting LDL-c should be enhanced in patients with T1DM to prevent IgAN.
Role of cystatin C in urogenital malignancy
Authors: Ding Li , Liu Zijie , Wang Junqi
Published: December 2022

Urogenital malignancy accounts for one of the major causes of cancer-related deaths globally. Numerous studies have investigated novel molecular markers in the blood circulation, tumor tissue, or urine in order to assist in the clinical identification of tumors at early stages, predict the response of therapeutic strategies, and give accurate prognosis assessment. As an endogenous inhibitor of lysosomal cysteine proteinases, cystatin C plays an integral role in diverse processes. A substantial number of studies have indicated that it may be such a potential promising biomarker. Therefore, this review was intended to provide a detailed overview of the role of cystatin C in urogenital malignancy.
Gonadotropin-releasing hormone analogue and recombinant human growth hormone treatment for idiopathic central precocious puberty in girls
Authors: Shi Yuzhen , Ma Ziyi , Yang Xi , Ying Yanqin , Luo Xiaoping , Hou Ling
Published: December 2022

Purpose

To investigate the effectiveness and safety of gonadotropin-releasing hormone analogue (GnRHa) in combination with recombinant human growth hormone (rhGH) in girls with central precocious puberty (CPP).

Methods

Clinical data of 80 girls diagnosed with idiopathic central precocious puberty (ICPP) between January 2017 and June 2021 were retrospectively analyzed. Treatment strategy involved GnRHa alone (group A: n=34) and GnRHa+rhGH (group B: n=46). Children’s heights (Ht), weights (Wt) and sex hormone levels were measured every 3 months after treatment and bone age (BA) every six months. Heights, growth velocity (GV), predicted adult height (PAH), weights, body mass index (BMI), sex hormone levels and bone age were compared between the two groups.

Results

Children in group B showed greater height gain at the 12th, 24th and 30th months after treatment (p<0.05) than those in group A, had faster growth rates in the first and second year following treatment (p<0.05) and better PAH (p<0.05). No statistical differences in weight or BMI were found between the two groups before treatment or at any time after treatment (p>0.05). Levels of LH and FSH were lower in both groups after treatment with no statistical differences between groups (p>0.05). The gap between bone age and chronological age gradually decreased in both groups and no abnormal progression of bone age or other adverse side effects occurred.

Conclusions

The combination of GnRHa with rhGH produced better height gains than GnRHa alone for patients with CPP. The gonadal axis was suppressed and progression of bone age delayed with good safety and efficacy.
The interactive effects of environmental gradient and dispersal shape spatial phylogenetic patterns
Authors: Jang Yun-Ting , Brännström Åke , Pontarp Mikael
Published: December 2022

Introduction

The emergence and maintenance of biodiversity include interacting environmental conditions, organismal adaptation to such conditions, and dispersal. To understand and quantify such ecological, evolutionary, and spatial processes, observation and interpretation of phylogenetic relatedness across space (e.g., phylogenetic beta diversity) is arguably a way forward as such patterns contain signals from all the processes listed above. However, it remains challenging to extract information about complex eco-evolutionary and spatial processes from phylogenetic patterns.

Methods

We link environmental gradients and organismal dispersal with phylogenetic beta diversity using a trait-based and eco-evolutionary model of diversification along environmental gradients. The combined effect of the environment and dispersal leads to distinct phylogenetic patterns between subsets of species and across geographical distances.

Results and discussion

Steep environmental gradients combined with low dispersal lead to asymmetric phylogenies, a high phylogenetic beta diversity, and the phylogenetic diversity between communities increases linearly along the environmental gradient. High dispersal combined with a less steep environmental gradient leads to symmetric phylogenies, low phylogenetic beta diversity, and the phylogenetic diversity between communities along the gradient increases in a sigmoidal form. By disentangling the eco-evolutionary mechanisms that link such interacting environment and dispersal effects and community phylogenetic patterns, our results improve understanding of biodiversity in general and help interpretation of observed phylogenetic beta diversity.
Differential expression of cyclins CCNB1 and CCNG1 is involved in the chondrocyte damage of kashin-beck disease
Authors: Setiwalidi Kaidiriye , Fu Jialei , Hei He , Nuer Shaniya , Zhang Feiyu , Chen Sijie , Liu Yanli , Chen Feihong , Li Shujin , Wang Chaowei , Wu Yifan , Gong Yi , Hu Minhan , Huang Ruitian , Liu Junyi , Zhang Tianxiao , Ning Yujie , Zhao Hongmou , Guo Xiong , Wang Xi
Published: December 2022

The purpose of this study was clarify the relationship between the differential expression of cyclins CCNB1 and CCNG1 and chondrocyte damage in Kashin-Beck disease. Systematic review and high-throughput sequencing of chondrocytes derived from Kashin-Beck disease patients were combined to identify the differentially expressed cyclins and cyclin-dependent kinase genes. In parallel, weaned SD rats were treated with low selenium for 4 weeks and then T-2 toxin for 4 weeks. Knee cartilage was collected to harvest chondrocytes for gene expression profiling. Finally, the protein expression levels of CCNB1 and CCNG1 were verified in knee cartilage tissue of Kashin-Beck disease patients and normal controls by immunohistochemical staining. The systematic review found 52 cartilage disease-related cyclins and cyclin-dependent kinase genes, 23 of which were coexpressed in Kashin-Beck disease, including 15 upregulated and 8 downregulated genes. Under the intervention of a low selenium diet and T-2 toxin exposure, CCNB1 (FC = 0.36) and CCNG1 (FC = 0.73) showed a downward expression trend in rat articular cartilage. Furthermore, compared to normal controls, CCNB1 protein in Kashin-Beck disease articular cartilage was 71.98% and 66.27% downregulated in the superficial and middle zones, respectively, and 12.06% upregulated in the deep zone. CCNG1 protein was 45.66% downregulated in the superficial zone and 12.19% and 9.13% upregulated in the middle and deep zones, respectively. The differential expression of cyclins CCNB1 and CCNG1 may be related to articular cartilage damage in Kashin-Beck disease.
Perinatal autopsy in Ghana: Healthcare workers knowledge and attitude
Authors: Swarray-Deen Alim , Attah Dzifa A. , Sefogah Promise E. , Oduro Nana E. , Nuamah Hanson G. , Nuamah Mercy A. , Adzadi Catherine , Oppong Samuel A.
Published: December 2022

Background

Perinatal mortality refers to stillbirths and early neonatal deaths. Stillbirth, the death of a foetus from 28 weeks or with a birth weight 1,000 g or above, and early neonatal deaths, the death of a new-born within 24 h of delivery, are among the most distressing global health problems, with approximately 2 million stillbirths occurring annually. Although a post-mortem examination of the stillborn baby is essential for understanding and learning the cause of stillbirth, many couples decline the procedure. Sub-Saharan Africa has one of the highest stillbirth rates in the world, yet there is a dearth of studies on post-mortem uptake from the region.

Aim

To explore healthcare professionals' views and perceptions of perinatal autopsy in Ghana.

Methods

Mixed-method approach consisted of semi-structured interviews and an electronic cross-sectional survey to evaluate the views and perceptions of healthcare professionals at Korle-Bu Teaching Hospital on autopsy for stillbirths and early neonatal deaths. Descriptive quantitative data were summarised in frequencies and percentages, and statistical results and descriptions were tabulated and coded in terms of types of barriers. For the qualitative aspect, the audio-taped interviews were transcribed, themes generated, and direct quotes and descriptions were coded for all knowledge, beliefs, attitudes and practices concerning the barriers and facilitators for post-mortem.

Results

Ninety-nine healthcare professionals participated. No participant had formal training regarding counselling for perinatal autopsy and 40% had “no idea” who is responsible for counselling and obtaining consent for a perinatal autopsy. Forty-four percent (44%) of the participants knew of only the “Conventional/ Full” autopsy and <4% were aware of less invasive methods of performing an autopsy. Qualitative data showed healthcare worker influence, religious and financial considerations impede the implementation of perinatal autopsies. Despite the low uptake of perinatal autopsies, interviews from healthcare workers suggest acceptance rates would improve if parents knew about different options, especially less invasive procedures.

Conclusion

At Ghana's largest referral centre, perinatal autopsy counselling and uptake are at extremely low levels. Most healthcare professionals have little knowledge, skills, and capacity to advise parents regarding perinatal autopsies. Training is needed to update the workforce on recommended perinatal autopsy practices.
Biology and clinical relevance of follicular cytotoxic T cells
Authors: Lv Yuqi , Ricard Laure , Gaugler Béatrice , Huang He , Ye Yishan
Published: December 2022

Follicular cytotoxic T (Tfc) cells are a newly identified subset of CD8+ T cells enriched in B cell follicles and their surroundings, which integrate multiple functions such as killing, memory, supporting and regulation. Tfc cells share similarities with follicular helper T (Tfh) cells, conventional cytotoxic CD8+ T (Tc cells)cells and follicular regulatory T (Tfr) cells, while they express distinct transcription factors, phenotype, and perform different functions. With the participation of cytokines and cell-cell interactions, Tfc cells modulate Tfh cells and B cells and play an essential role in regulating the humoral immunity. Furthermore, Tfc cells have been found to change in their frequencies and functions during the occurrence and progression of chronic infections, immune-mediated diseases and cancers. Strategies targeting Tfc cells are under investigations, bringing novel insights into control of these diseases. We summarize the characteristics of Tfc cells, and introduce the roles and potential targeting modalities of Tfc cells in different diseases.
A U-shaped association of tracheostomy timing with all-cause mortality in mechanically ventilated patients admitted to the intensive care unit: A retrospective cohort study
Authors: Chen Jing-Ran , Gao Hao-Ran , Yang Yan-Lin , Wang Yan , Zhou Yi-Min , Chen Guang-Qiang , Li Hong-Liang , Zhang Linlin , Zhou Jian-Xin
Published: December 2022

Objectives

To evaluate the association of tracheostomy timing with all-cause mortality in patients with mechanical ventilation (MV).

Method

It’s a retrospective cohort study. Adult patients undergoing invasive MV who received tracheostomy during the same hospitalization based on the Medical Information Mart for Intensive Care-III (MIMIC-III) database, were selected. The primary outcome was the relationship between tracheostomy timing and 90-day all-cause mortality. A restricted cubic spline was used to analyze the potential non-linear correlation between tracheostomy timing and 90-day all-cause mortality. The secondary outcomes included free days of MV, incidence of ventilator-associated pneumonia (VAP), free days of analgesia/sedation in the intensive care unit (ICU), length of stay (LOS) in the ICU, LOS in hospital, in-ICU mortality, and 30-day all-cause mortality.

Results

A total of 1,209 patients were included in this study, of these, 163 (13.5%) patients underwent tracheostomy within 4 days after intubation, while 647 (53.5%) patients underwent tracheostomy more than 11 days after intubation. The tracheotomy timing showed a U-shaped relationship with all-cause mortality, patients who underwent tracheostomy between 5 and 10 days had the lowest 90-day mortality rate compared with patients who underwent tracheostomy within 4 days and after 11 days [84 (21.1%) vs. 40 (24.5%) and 206 (31.8%), P < 0.001].

Conclusion

The tracheotomy timing showed a U-shaped relationship with all-cause mortality, and the risk of mortality was lowest on day 8, but a causal relationship has not been demonstrated.
The spatio-temporal properties of calcium transients in hippocampal pyramidal neurons in vitro
Authors: Shkryl Vyacheslav M.
Published: December 2022

The spatio-temporal properties of calcium signals were studied in cultured pyramidal neurons of the hippocampus using two-dimensional fluorescence microscopy and ratiometric dye Fura-2. Depolarization-induced Ca2+ transients revealed an asynchronous delayed increase in free Ca2+ concentration. We found that the level of free resting calcium in the cell nucleus is significantly lower compared to the soma, sub-membrane, and dendritic tree regions. Calcium release from the endoplasmic reticulum under the action of several stimuli (field stimulation, high K+ levels, and caffeine) occurs in all areas studied. Under depolarization, calcium signals developed faster in the dendrites than in other areas, while their amplitude was significantly lower since larger and slower responses inside the soma. The peak value of the calcium response to the application of 10 mM caffeine, ryanodine receptors (RyRs) agonist, does not differ in the sub-membrane zone, central region, and nucleus but significantly decreases in the dendrites. In the presence of caffeine, the delay of Ca2+ signals between various areas under depolarization significantly declined. Thirty percentage of the peak amplitude of Ca2+ transients at prolonged electric field stimulation corresponded to calcium release from the ER store by RyRs, while short-term stimulation did not depend on them. 20 μM dantrolene, RyRs inhibitor, significantly reduces Ca2+ transient under high K+ levels depolarization of the neuron. RyRs-mediated enhancement of the Ca2+ signal is more pronounced in the central part and nucleus compared to the sub-membrane or dendrites regions of the neuron. In summary, using the ratiometric imaging allowed us to obtain additional information about the involvement of RyRs in the intracellular dynamics of Ca2+ signals induced by depolarization or electrical stimulation train, with an underlying change in Ca2+ concentration in various regions of interest in hippocampal pyramidal neurons.
Oncologic impact of delay between diagnosis and radical nephroureterectomy
Authors: Wu Kuan-Hsien , Chang Chao-Hsiang , Wu Hsi-Chin , Huang Steven K. , Liu Chien-Liang , Yang Cheng-Kuang , Li Jian-Ri , Tseng Jen-Shu , Lin Wun-Rong , Yu Chih-Chin , Lo Chi-Wen , Huang Chao-Yuan , Chen Chung-Hsin , Tsai Chung-You , Cheng Pai-Yu , Jiang Yuan-Hong , Lee Yu-Khun , Chen Yung-Tai , Yeh Ting-Chun , Lin Jen-Tai , Tsai Yao-Chou , Hsueh Thomas Y. , Chiang Bing-Juin , Chiang Yi-De , Lin Wei-Yu , Jou Yeong-Chin , Pang See-Tong , Ke Hung-Lung
Published: December 2022

Purpose

This study aimed to evaluate the oncological outcome of delayed surgical wait time from the diagnosis of upper tract urothelial carcinoma (UTUC) to radical nephroureterectomy (RNU).

Methods

In this multicenter retrospective study, medical records were collected between 1988 and 2021 from 18 participating Taiwanese hospitals under the Taiwan UTUC Collaboration Group. Patients were dichotomized into the early (≤90 days) and late (>90 days) surgical wait-time groups. Overall survival, disease-free survival, and bladder recurrence-free survival were calculated using the Kaplan–Meier method and multivariate Cox regression analysis. Multivariate analysis was performed using stepwise linear regression.

Results

Of the 1251 patients, 1181 (94.4%) were classifed into the early surgical wait-time group and 70 (5.6%) into the late surgical wait-time group. The median surgical wait time was 21 days, and the median follow-up was 59.5 months. Our study showed delay-time more than 90 days appeared to be associated with worse overall survival (hazard ratio [HR] 1.974, 95% confidence interval [CI] 1.166−3.343, p = 0.011), and disease-free survival (HR 1.997, 95% CI 1.137−3.507, p = 0.016). This remained as an independent prognostic factor after other confounding factors were adjusted. Age, ECOG performance status, Charlson Comorbidity Index (CCI), surgical margin, tumor location and adjuvant systemic therapy were independent prognostic factors for overall survival. Tumor location and adjuvant systemic therapy were also independent prognostic factors for disease-free survival.

Conclusions

For patients with UTUC undergoing RNU, the surgical wait time should be minimized to less than 90 days. Prolonged delay times may be associated with poor overall and disease-free survival.
Analysis of the regulatory mechanisms of prognostic immune factors in thyroid cancer
Authors: Tian Yin , Xie Tao , Sun Xue
Published: December 2022

Objective

To explore the regulatory mechanism of immune prognostic factors in thyroid cancer.

Methods

Based on the TCGA database and GEO database, this study used bioinformatics methods to study the potential regulatory mechanism of thyroid cancer prognosis, analyzed the differentially expressed genes and differential miRNAs between thyroid cancer and normal paracancerous tissues by R software, and constructed lasso risk factors. The immune prognostic factors of thyroid cancer were obtained from the model, and the miRDB website was used to predict the possibility of differential miRNA target binding of the immune prognostic factors and correlation analysis was performed, and finally verified by cell experiments.

Results

There were 1413 differentially expressed genes between thyroid cancer and normal paracancerous tissues, among which 21 immune-related genes were prognostic factors with significant differences in expression; lasso risk model obtained AKAP12, APOC1, TIMP3, ADAMTS9, ANK2, HTRA3, SYNDIG1 , ADAMTS5 and DACT1 were nine prognostic factors. A total of 58 differential miRNAs were found in thyroid cancer tissues and non-cancerous tissues. The possibility of differential miRNA targeting and binding of immune prognostic factors on the miRDB website and cell experiments was analyzed.

Conclusions

The potential miRNA regulatory mechanism of immune prognostic factors in thyroid cancer has been explored.
Genome-wide identification of genes encoding cystathionine beta synthase domain-containing proteins in wheat and its relationship with anther male sterility under heat stress
Authors: Liu Hongzhan , Wang Qi , Xie Liuyong , Xu Kedong , Zhang Fuli , Ruan Xianle , Li Lili , Tan Guangxuan
Published: December 2022

Cystathionine beta synthase (CBS) domains containing proteins (CDCPs) plays an important role in plant development through regulation of the thioredoxin system, as well as its ability to respond to biotic and abiotic stress conditions. Despite this, no systematic study has examined the wheat CBS gene family and its relation to high temperature-induced male sterility. In this study, 66 CBS family members were identified in the wheat genome, and their gene or protein sequences were used for subsequent analysis. The TaCBS gene family was found to be unevenly distributed on 21 chromosomes, and they were classified into four subgroups according to their gene structure and phylogeny. The results of collinearity analysis showed that there were 25 shared orthologous genes between wheat, rice and Brachypodium distachyon, and one shared orthologous gene between wheat, millet and barley. The cis-regulatory elements of the TaCBS were related to JA, IAA, MYB, etc. GO and KEGG pathway analysis identified these TaCBS genes to be associated with pollination, reproduction, and signaling and cellular processes, respectively. A heatmap of wheat plants based on transcriptome data showed that TaCBS genes were expressed to a higher extent in spikelets relative to other tissues. In addition, 29 putative tae-miRNAs were identified, targeting 41 TaCBS genes. Moreover, qRT-PCR validation of six TaCBS genes indicated their critical role in anther development, as five of them were expressed at lower levels in heat-stressed male sterile anthers than in Normal anthers. Together with anther phenotypes, paraffin sections, starch potassium iodide staining, and qRT-PCR data, we hypothesized that the genes encoding CDCPs has a very important connection with the heat-stressed sterility process in wheat, and these data provide a basis for further insight into their relationship.
Executive function and effortful control—Similar and different evidence from big data analysis
Authors: Chae Soo Eun
Published: December 2022

Introduction

The current study explored commonalities and similarities between executive function (EF) and effortful control (EC).

Methods

The major empirical studies published between 2013 and 2022 in the World of Science (WoS) was collected. The bibliographic information was systematically analyzed.

Results and discussion

(1) EC is the efficiency of executive attention that incorporates inhibitory control (IC), attentional control, activation mainly related to temperament. On the other hand, EF is the efficiency of self-directed action that encompasses IC, working memory (WM), and shifting/cognitive flexibility in particular focuses on the cognitive aspect. (2) EF research has overwhelmingly outnumbered EC research (2,000 EF studies vs. 50 EC studies per year). (3) According to a co-word analysis with keyword co-occurrences, the subject of preschool students and individual differences co-occurred in EF studies. (4) EC usually occurs with working memory and early childhood. In the more detailed analysis of the articles, the EF and EC studies used younger subject groups than older subject groups. EC studies were especially likely to use subjects in early childhood. (5) The Delis–Kaplan Tests of Executive Functioning System (D-KEFS) was the most commonly used test for EF. In contrast, the EC used self-report surveys such as the Adolescent Temperament Questionnaire (ATQ). This research illustrates and discusses key findings in the EC and EF data and provides suggestions for future study directions.
Smartphone addiction is more harmful to adolescents than Internet gaming disorder: Divergence in the impact of parenting styles
Authors: Li Zhao-kang , Shi Li-juan , Cai Xin-lu
Published: December 2022

Background

The adverse effects of smartphone addiction (SPA) and Internet gaming disorder (IGD) on adolescents’ mental health have been widely recognized. However, the influence of parenting styles on these high-risk Internet use behaviors of adolescents still remain elusive. Aiming to identify preventable patterns for adolescents with SPA or IGD, this study compared the mental health status between adolescents with SPA and IGD and used path analysis to confirm actual effects of parenting styles on SPA and IGD.

Methods

Participants were enrolled at a junior high school in Hunan Province and a senior high school in Shanxi Province, China [n = 3,049, female (male): 50.5% (49.5%), mean age = 15.68 ± 1.54]. All participants reported their socio-demographic characteristics and undertook standardized assessments of SPA, IGD, parenting styles, depression, anxiety, insomnia, self-control, and support utilization.

Results

High levels of parental care and low levels of parental overprotection benefited adolescents’ mental health with SPA and IGD. However, despite having a more positive parenting style, adolescents with only SPA showed more severe mental health problems than adolescents with only IGD. Furthermore, the results showed that the parenting style of encouraging autonomy might be a protective factor against IGD, but it might reinforce SPA indirectly by reducing abilities of support utilization and self-control in whole sample.

Conclusion

Compared to IGD, SPA which included different kinds of Internet addiction behaviors, was more hazardous for adolescents’ mental health. The divergent effects of an autonomy-encouraging parenting style on SPA and IGD may reflect the different impacts of self-control in different types of Internet addiction.
Influencing factors of serum magnesium in CKD5 patients: A multicenter study in southern China
Authors: Wang Jiali , Lin Shujun , Li Hong-Yan , Tang Wenzhuang , Liu Yiping , Zhou Tianbiao
Published: December 2022

Introduction

Magnesium (Mg) disturbances are related to cardiac, bone, and renal patient mortality. In this study, we compared biochemical markers in hemodialysis (HD) and peritoneal dialysis (PD) patients and explored the influencing factors of serum Mg in stage 5 chronic kidney disease (CKD5) patients.

Material and methods

All 598 patients with CKD5 from three medical centers in South China were recruited into this prospective cohort study from March 1, 2018, to January 31, 2021. Our study recorded the clinical characteristics and laboratory data of the patients.

Results

Hemodialysis patients (0.99 ± 0.19 mmol/L) had a higher mean serum Mg level than PD patients (0.86 ± 0.20 mmol/L; p < 0.01). Regression analysis showed that only corrected calcium (Ca), phosphate (P), Ca/Mg, Ca × P, albumin (Alb), total protein and creatine (Cr) predicted Mg levels in CKD5 patients (p < 0.01). Ca/Mg predicts hypomagnesemia with 78% sensitivity and 85% specificity in CKD5 patients. The AUC value corresponding to Ca/Mg was 0.88.

Conclusions

This multicenter study in southern China showed that for all CKD5 patients, corrected Ca and Alb had a significant positive effect on serum Mg, while Ca/Mg had a significant negative effect on serum Mg. In 123 HD patients, Ca × P was positively associated with Mg while Ca/Mg and P were negatively associated with Mg. In 398 PD patients, Ca × P, Alb, and total protein were positively associated with Mg while Ca/Mg and P were negatively associated with Mg. In 77 non-dialysis patients, corrected Ca, Cr, and total protein were positively associated with Mg while Ca/Mg was negatively associated with Mg. Furthermore, Ca/Mg might be another useful technique to monitor blood Mg levels in CKD5 patients.

Clinical trial registration

ChiCTR1800014557.
Research trends in healthcare and hospital administration in Japan: Content analyses of article titles in the journal of the Japan society for healthcare administration
Authors: Moteki Yasutoshi
Published: December 2022

This study quantitatively analyzed healthcare administration studies in Japan using text mining, focusing on articles published during 1994–2021 in the Journal of the Japan Society for Healthcare Administration (prior to 2008, the journal was called Hospital Administration). Both the co-occurrence network and the correspondence analysis (these are extracted words that refer to the two systems) demonstrate two major changes: (1) the introduction of the long-term care insurance system, which was enacted in 1997 and came into effect in 2000, and (2) the introduction of the late-stage medical care system for the elderly in 2008, both of which had a significant impact on the Japanese public health and welfare system. Co-occurrence network and correspondence analysis were conducted to understand changes in research interests. The analysis used two time periods following a change in the journal's name in 2008. To readily comprehend changing research trends, 10-year segments were considered, resulting in three time periods. The research features and trends during the aforementioned periods were examined using correspondence analysis. Configuration figures derived from this analysis plotted time transition (first dimension) against certain abstract/concrete situations (second dimension). The extracted words displayed in the configuration maps at the axes' intersection were patient, survey, and evaluation. They revealed no distinctive features compared with other words and were commonly used in article titles within this journal during each period. The following results were obtained from the correspondence analysis: first, changes in the geriatric care system of public medical insurance and the introduction of the long-term care insurance system in 2000 were expressed in the characteristics of the extracted words; second, in the 14 years after the journal's name changed, published studies frequently referred to the roles of doctors, nurses, and other healthcare professionals. A chi-squared test on these extracted words and the period classification confirmed a statistically significant relationship between them.
History of falls and fear of falling are predictive of future falls: Outcome of a fall rate model applied to the Swiss CHEF Trial cohort
Authors: Wapp Christina , Mittaz Hager Anne-Gabrielle , Hilfiker Roger , Zysset Philippe
Published: December 2022

Background: A third of adults aged 65 years and older fall every year, and falls are a common cause of unintentional injuries. Accurate identification of people at risk of falling is an important step in the implementation of preventive strategies.

Objective: Our aim was to investigate the association of fall risk factors with number of reported falls in terms of incidence rate ratios and to develop a fall rate prediction model.

Methods: In the randomized controlled trial Swiss CHEF, multiple fall risk variables were assessed in community-dwelling older adults at baseline examination, including age, sex, body mass index, fear of falling, number of falls during the prior 12 months, scores on several physical performance tests, comorbidities, and quality of life. Over the following 6 months, interventions were administered in the form of three home-based exercise programs. Participants were subsequently followed up for another 6 months. Falls were reported prospectively using monthly calendars. Incidence rate ratios were derived via negative binomial regression models. Variable selection for the prediction model was conducted using backward elimination and the least absolute shrinkage and selection operator method; the model with the smallest prediction error was then identified.

Results: Associations with the number of reported falls were found for number of prior falls, fear of falling, balance and gait deficits, and quality of life. The final model was derived via backward elimination, and the predictors included were prior number of falls and a measure of fear of falling.

Outcome: Number of prior falls and fear of falling can be used as predictors in a personalized fall rate estimate for community-dwelling older adults. Recurrent fallers having experienced four or more falls are especially at risk of falling again.
The Turing Teacher: Identifying core attributes for AI learning in K-12
Authors: Pelaez Alexander , Jacobson Amal , Trias Kara , Winston Elaine
Published: December 2022

Introduction

Artificial intelligence in the educational domain has many uses; however, using AI specifically to enhance education and teaching in a K-12 environment poses the most significant challenges to its use. Beyond usage and application, the quality of the education is made even more arduous due to the dynamics of teaching primary and secondary school children, whose needs far exceed mere fact recollection. Utilizing prior research using AI in education and online education in the K-12 space, we explore some of the hurdles that AI applications face in K-12 teaching and provide core attributes for a “Turing Teacher,” i.e., an AI powered technology for learning, specifically targeting the K-12 space.

Methods

Using a survey, which included qualitative responses during the implementation of online learning during the Covid Pandemic, we analyze the results using univariate and multivariate tests and analyzed the qualitative responses to create core attributes needed for AI powered teaching technology.

Results

The results present the challenges faced by any technology in an education setting and show that AI technology must help overcome negative feelings about technology in education. Further, the core attributes identified in the research must be addressed from the three stakeholder perspectives of teachers, parents and students.

Discussion

We present our findings and lay the groundwork for future research in the area of AI powered education. The Turing Teacher must be able to adapt and collaborate with real teachers and address the varying needs of students. In addition, we explore the use of AI technology as a means to close the digital divide in traditionally disadvantaged communities.
Ex-Cit XR: Expert-elicitation and validation of Extended Reality visualisation and interaction techniques for disengaging and transitioning users from immersive virtual environments
Authors: Piumsomboon Thammathip , Ong Gavin , Urban Cameron , Ens Barrett , Topliss Jack , Bai Xiaoliang , Hoermann Simon
Published: December 2022

This research explores visualisation and interaction techniques to disengage users from immersive virtual environments (IVEs) and transition them back to the Augmented Reality mode in the real world. To gain a better understanding and novel ideas, we invited eleven Extended Reality (XR) experts to participate in an elicitation study to design such techniques for disengagement. From the elicitation study, we elicited a total of 132 techniques for four different scenarios of IVEs: Narrative-driven, Social-platform, Adventure Sandbox, and Fast-paced Battle experiences. Through extracted keywords and thematic analysis, we classified the elicited techniques into six categories of Activities, Breaks, Cues, Degradations, Notifications, and Virtual Agents. We shared our analyses on users’ intrinsic motivation to engage in different experiences, subjective ratings of four design attributes in designing the disengagement techniques, Positive and Negative Affect Schedules, and user preference. In addition, we gave the design patterns found and illustrated the exemplary user cases of Ex-Cit XR. Finally, we conducted an online survey to preliminarily validate our design recommendations. We proposed the SPINED behavioural manipulation spectrum for XR disengagement to guide how the systems can strategically escalate to disengage users from an IVE.
Geochemical Characterization of Nyamyumba Hot Springs, Northwest Rwanda
Authors: Hategekimana Francois , Mugerwa Theophile , Nsengiyumva Cedrick , Byiringiro Fils Vainqueur , Rwatangabo Digne Edmond Rwabuhungu
Published: December 2022

A hot spring is a hot water source that naturally occurs on the surface of the Earth from underground; it is typically heated by subterranean volcanic activity and the local underground geothermal gradient. There are four main hot springs in Rwanda, such as: Kalisimbi, Bugarama, Kinigi, and Nyamyumba, formerly named Gisenyi hot springs. It is often believed that soaking in a hot spring is a great way to naturally detox human skin. This research focuses on the geochemical analysis of Nyamyumba hot springs, located near the fresh water supply of Lake Kivu, with the purpose of understanding its healing capacity and safety. The Nyamyumba hot springs are located in the western branch of the East African Rift System, near the Virunga volcanic complex, which explains the rising and heating mechanism of the water. The concentrations of sulfate, iron, ammonia, silica, and phosphate, and the conductivity, alkalinity, and salinity of the water were measured using standard procedures. The results showed that the hot spring water has higher concentrations of chemicals compared to the Lake Kivu water, and the geochemistry of these hot springs may be associated with rock dissolution by hot water. The measured parameters were compared with World Health Organization (WHO) standards for recreational waters, and it has been identified that the Nyamyumba hot springs are safe to use for swimming and therapeutic activities.
Synucleins: New Data on Misfolding, Aggregation and Role in Diseases
Authors: Surguchov Andrei , Surguchev Alexei
Published: December 2022

The synucleins are a family of natively unfolded (or intrinsically unstructured) proteins consisting of α-, β-, and γ-synuclein involved in neurodegenerative diseases and cancer. The current number of publications on synucleins has exceeded 16.000. They remain the subject of constant interest for over 35 years. Two reasons explain this unchanging attention: synuclein’s association with several severe human diseases and the lack of understanding of the functional roles under normal physiological conditions. We analyzed recent publications to look at the main trends and developments in synuclein research and discuss possible future directions. Traditional areas of peak research interest which still remain high among last year’s publications are comparative studies of structural features as well as functional research on of three members of the synuclein family. Another popular research topic in the area is a mechanism of α-synuclein accumulation, aggregation, and fibrillation. Exciting fast-growing area of recent research is α-synuclein and epigenetics. We do not present here a broad and comprehensive review of all directions of studies but summarize only the most significant recent findings relevant to these topics and outline potential future directions.
Deuterohemin-Ala-His-Thr-Val-Glu-Lys (DhHP-6) Mimicking Enzyme as Synergistic Antioxidant and Anti-Inflammatory Material for Periodontitis Therapy
Authors: Yan Jiaqing , Liu Min , Zhang Yan , Zhu Ying , Chen Qiuyan , Yang Yimeng , Hu Min , Yu Huimei
Published: December 2022

Periodontitis is an inflammatory disease induced by plaque microorganisms. In the clinic, antibiotic assistant periodontal mechanical therapy is the most effective therapy for the treatment of periodontitis. However, the drug resistance of the antibiotics and the repeated coming and diminishing of the disorder of oxidation–reduction balance in the inflammatory tissue could not meet the high requirements for periodontic health control in long periods. Deuterohemin-ala-his-thr-val-glu-lys (DhHP-6) is a biomimetic oxidase-mimicking enzyme that simulates the reactive oxygen radical scavenger function of heme by synthesizing the new molecular material following the key structure and amino acid sequence of heme. In this article, we report the antioxidant and anti-inflammatory properties of DhHP-6 by building a inflammatory model for human gingival fibroblasts (HGFs) stimulated by lipolysaccharide (LPS) and its effects on periodontitis in Wistar rats. DhHP-6 reduced the oxidative stress of HGFs by increasing the amount of the reductase species of glutathione (GSH) and catalase (CAT) while decreasing the amount of oxidase species of malonaldehyde (MDA) and reactive oxygen species (ROS). DhHP-6 had a dose-dependent protective effect on alveolar bone absorption in rats with periodontitis, enhanced antioxidant capacity, and reduced inflammation. As determined by Micro-CT scanning, DhHP-6 reduced alveolar bone loss and improved the bone structure of the left maxillary first molar of rats. There were no obvious morphological and histological differences in the rat organs with or without DhHP-6 treatment. These results suggest that DhHP-6 can be used to treat periodontitis by increasing the expression levels of antioxidant enzymes and antioxidants in systemic and local tissues, thereby reducing levels of oxidation products and cyto-inflammatory factors. The synergistic antioxidant and anti-inflammatory effects of DhHP-6 suggest that there are promising applications of this biomimetic enzyme molecular material for the next generation of agents for periodontitis therapy.