Carbon Black CB-EDA Nanoparticles in Hepatocytes: Changes in the Oxidative Stress Pathway
Authors:   Laboratório de Inflamação e Doenças Infecciosas, Departamento de Morfologia e Patologia, Universidade Federal de São Carlos, São Carlos, Brazil,, Rodolpho Joice Margareth de Almeida , Godoy Krissia Franco de ,   Laboratório de Inflamação e Doenças Infecciosas, Departamento de Morfologia e Patologia, Universidade Federal de São Carlos, São Carlos, Brazil,, Brassolatti Patricia ,   Laboratório de Inflamação e Doenças Infecciosas, Departamento de Morfologia e Patologia, Universidade Federal de São Carlos, São Carlos, Brazil,, Fragelli Bruna Dias de Lima ,   Laboratório de Inflamação e Doenças Infecciosas, Departamento de Morfologia e Patologia, Universidade Federal de São Carlos, São Carlos, Brazil,, Camillo Luciana ,   Laboratório de Inflamação e Doenças Infecciosas, Departamento de Morfologia e Patologia, Universidade Federal de São Carlos, São Carlos, Brazil,, Castro Cynthia Aparecida de ,   Laboratório de Inflamação e Doenças Infecciosas, Departamento de Morfologia e Patologia, Universidade Federal de São Carlos, São Carlos, Brazil,, Nogueira Camila Tita ,   PhD in Biotechnology, Assis Marcelo ,   Department of Analytical and Physical Chemistry, University Jaume I (UJI), 12071, Castelló, Spain, Centro de Pesquisa Leopoldo Américo Miguez de Mello CENPES/Petrobras, Rio de Janeiro, Brazil, Speglich Carlos ,   Centro de Pesquisa Leopoldo Américo Miguez de Mello CENPES/Petrobras, Rio de Janeiro, Brazil,, Longo Elson ,   Centro de Desenvolvimento de Materiais Funcionais, Departamento de Química, Universidade Federal de São Carlos, São Carlos, Brazil., Anibal Fernanda de Freitas ,   Laboratório de Inflamação e Doenças Infecciosas, Departamento de Morfologia e Patologia, Universidade Federal de São Carlos, São Carlos, Brazil,
Published: December 2022

Background/Aims: Carbon Black (CB) is the most widely produced and commercially used nanocarbon. Growing evidence links nanomaterials to adverse effects, arising from their large surface area capable of interacting with biological systems. Due to the variety of applications and human exposures to nanoparticles, it is important to assess the potential health risk and understand the underlying mechanisms of their toxicity. The present study aims to investigate the cytotoxic effect of the Carbon black nanoparticle covalently linked to ethylenediamine (CB-EDA) on AML-12 cells, a lineage of murine hepatocytes. Methods: For this, the cells were exposed to CB-EDA for 24h, at different concentrations of the nanoparticle (1, 50, 250, 500 and 1000 μg/mL). Effects on cell viability were evaluated using MTT and neutral red dye tests and analysis of changes in cell morphology. Furthermore, the oxidative profile (levels of reactive oxygen species - ROS and nitrogen - RNS) and inflammatory profile (production of IL-6 and TNF-α) were investigated. Results: The results show that CB-EDA nanoparticle causes a reduction in cell viability at concentrations of 250, 500 and 1000 μg/mL after the exposure period. There was a significant increase in the production of ROS, RNS and pro-inflammatory interleukin TNF-α. Conclusion: The data suggest that CB-EDA nanoparticle has a cytotoxic potential in AML-12 hepatocytes, evidenced by the induction of oxidative stress and secretion of inflammatory cytokines, with a consequent decrease in cell viability.