bluuebunny/crossref_metadata_2025 huggingface dataset:
Considering Section Balance in an Integer Optimization Model for the Curriculum-Based Course Timetabling Problem
Authors: Palma Cristian D. , Bornhardt Patrick
Published: October 2020

University course timetabling is a complex and time-consuming duty that every educational institution faces regularly. It consists of scheduling a set of lectures in predefined time slots so as to avoid student conflicts, meet teacher and room availability, and manage several institution-specific operational rules. In this paper, we schedule courses based on a curriculum, that is, before the students’ registration. Unlike other curriculum-based models, the proposed model considers two practical aspects when managing the conflicts between lectures: (i) it schedules sections of subjects so that each section is evenly likely to be registered by the students, and (ii) it considers the failure rates and periodicity a subject is taught. We present a multi-objective integer programming model that maximizes the use of specific time slots, the symmetry in which the lectures of a course are scheduled during a week, and the flexibility for straggler students to take courses. The model is solved using commercial software, and it is applied to a real course-timetabling problem. We show the advantages of its use by comparing the model’s solution with the actual solution obtained by the manual scheduling.
On Tail Dependence and Multifractality
Authors: Avdulaj Krenar , Kristoufek Ladislav
Published: October 2020

We study whether, and if yes then how, a varying auto-correlation structure in different parts of distributions is reflected in the multifractal properties of a dynamic process. Utilizing the quantile autoregressive process with Gaussian copula using three popular estimators of the generalized Hurst exponent, our Monte Carlo simulation study shows that such dynamics translate into multifractal dynamics of the generated series. The tail-dependence of the auto-correlations forms strong enough non-linear dependencies to be reflected in the estimated multifractal spectra and separated from the case of the standard auto-regressive process. With a quick empirical example from financial markets, we argue that the interaction is more important for the asymmetric tail dependence. In addition, we discuss and explain the often reported paradox of higher multifractality of shuffled series compared to the original financial series. In short, the quantile-dependent auto-correlation structures qualify as sources of multifractality and they are worth further theoretical examination.
Further Properties of Quantum Spline Spaces
Authors: Budakçı Gülter , Oruç Halil
Published: October 2020

We construct q-B-splines using a new form of truncated power functions. We give basic properties to show that q-B-splines form a basis for quantum spline spaces. On the other hand, we derive algorithmic formulas for 1/q-integration and 1/q-differentiation for q-spline functions. Moreover, we show a way to find the polynomial pieces on each interval of a q-spline function.
Implicit Three-Point Block Numerical Algorithm for Solving Third Order Initial Value Problem Directly with Applications
Authors: Allogmany Reem , Ismail Fudziah
Published: October 2020

Recently, direct methods that involve higher derivatives to numerically approximate higher order initial value problems (IVPs) have been explored, which aim to construct numerical methods with higher order and very high precision of the solutions. This article aims to construct a fourth and fifth derivative, three-point implicit block method to tackle general third-order ordinary differential equations directly. As a consequence of the increase in order acquired via the implicit block method of higher derivatives, a significant improvement in efficiency has been observed. The new method is derived in a block mode to simultaneously evaluate the approximations at three points. The derivation of the new method can be easily implemented. We established the proposed method’s characteristics, including order, zero-stability, and convergence. Numerical experiments are used to confirm the superiority of the method. Applications to problems in physics and engineering are given to assess the significance of the method.
Kidney and Renal Tumor Segmentation Using a Hybrid V-Net-Based Model
Authors: Türk Fuat , Lüy Murat , Barışçı Necaattin
Published: October 2020

Kidney tumors represent a type of cancer that people of advanced age are more likely to develop. For this reason, it is important to exercise caution and provide diagnostic tests in the later stages of life. Medical imaging and deep learning methods are becoming increasingly attractive in this sense. Developing deep learning models to help physicians identify tumors with successful segmentation is of great importance. However, not many successful systems exist for soft tissue organs, such as the kidneys and the prostate, of which segmentation is relatively difficult. In such cases where segmentation is difficult, V-Net-based models are mostly used. This paper proposes a new hybrid model using the superior features of existing V-Net models. The model represents a more successful system with improvements in the encoder and decoder phases not previously applied. We believe that this new hybrid V-Net model could help the majority of physicians, particularly those focused on kidney and kidney tumor segmentation. The proposed model showed better performance in segmentation than existing imaging models and can be easily integrated into all systems due to its flexible structure and applicability. The hybrid V-Net model exhibited average Dice coefficients of 97.7% and 86.5% for kidney and tumor segmentation, respectively, and, therefore, could be used as a reliable method for soft tissue organ segmentation.
Fractional-Order Integro-Differential Multivalued Problems with Fixed and Nonlocal Anti-Periodic Boundary Conditions
Authors: Alsaedi Ahmed , P. Agarwal Ravi , K. Ntouyas Sotiris , Ahmad Bashir
Published: October 2020

This paper studies a new class of fractional differential inclusions involving two Caputo fractional derivatives of different orders and a Riemann–Liouville type integral nonlinearity, supplemented with a combination of fixed and nonlocal (dual) anti-periodic boundary conditions. The existence results for the given problem are obtained for convex and non-convex cases of the multi-valued map by applying the standard tools of the fixed point theory. Examples illustrating the obtained results are presented.
Novel Parametric Solutions for the Ideal and Non-Ideal Prouhet Tarry Escott Problem
Authors: Raghavendran Srikanth , Narayanan Veena
Published: October 2020

The present study aims to develop novel parametric solutions for the Prouhet Tarry Escott problem of second degree with sizes 3, 4 and 5. During this investigation, new parametric representations for integers as the sum of three, four and five perfect squares in two distinct ways are identified. Moreover, a new proof for the non-existence of solutions of ideal Prouhet Tarry Escott problem with degree 3 and size 2 is derived. The present work also derives a three parametric solution of ideal Prouhet Tarry Escott problem of degree three and size two. The present study also aimed to discuss the Fibonacci-like pattern in the solutions and finally obtained an upper bound for this new pattern.
Efficient Parallel Implementations of LWE-Based Post-Quantum Cryptosystems on Graphics Processing Units
Authors: An SangWoo , Seo Seog Chung
Published: October 2020

With the development of the Internet of Things (IoT) and cloud computing technology, various cryptographic systems have been proposed to protect increasing personal information. Recently, Post-Quantum Cryptography (PQC) algorithms have been proposed to counter quantum algorithms that threaten public key cryptography. To efficiently use PQC in a server environment dealing with large amounts of data, optimization studies are required. In this paper, we present optimization methods for FrodoKEM and NewHope, which are the NIST PQC standardization round 2 competition algorithms in the Graphics Processing Unit (GPU) platform. For each algorithm, we present a part that can perform parallel processing of major operations with a large computational load using the characteristics of the GPU. In the case of FrodoKEM, we introduce parallel optimization techniques for matrix generation operations and matrix arithmetic operations such as addition and multiplication. In the case of NewHope, we present a parallel processing technique for polynomial-based operations. In the encryption process of FrodoKEM, the performance improvements have been confirmed up to 5.2, 5.75, and 6.47 times faster than the CPU implementation in FrodoKEM-640, FrodoKEM-976, and FrodoKEM-1344, respectively. In the encryption process of NewHope, the performance improvements have been shown up to 3.33 and 4.04 times faster than the CPU implementation in NewHope-512 and NewHope-1024, respectively. The results of this study can be used in the IoT devices server or cloud computing service server. In addition, the results of this study can be utilized in image processing technologies such as facial recognition technology.
The Relationship between Changes in Organ-Tissue Mass and Sleeping Energy Expenditure Following Weight Change in College Sumo Wrestlers
Authors: Midorikawa Taishi , Tanaka Shigeho , Ando Takafumi , Konishi Masayuki , Ohta Megumi , Torii Suguru , Sakamoto Shizuo
Published: October 2020

Background and objectives: It has been well established that the resting energy expenditure (REE) for the whole body is the sum of the REE for each organ-tissue in young and middle-aged healthy adults. Based on these previous studies, although it is speculated that sleeping energy expenditure (SEE, which has small inter-individual variability) changes with a commensurate gain or reduction in the resting metabolic rate of each organ-tissue, it is unclear whether a change in organ-tissue masses is directly attributed to the fluctuation of SEE at present. This study aimed to assess the relationship between changes in organ-tissue mass and sleeping energy expenditure (SEE) following weight change in college Sumo wrestlers. This included blood analysis, which is related to energy expenditure. Materials and Methods: A total of 16 healthy male college Sumo wrestlers were recruited in this study. All measurements were obtained before and after weight change. Magnetic resonance imaging measurements were used to determine the volume of the skeletal muscle (SM), liver, and kidneys, and an indirect human calorimeter was used to determine SEE before and after weight change. Results: The change in body mass and SEE ranged between −8.7~9.5 kg, and −602~388 kcal/day. Moreover, changes in SM, liver, and kidneys ranged between −3.3~3.6 kg, −0.90~0.77 kg, and −0.12~0.07 kg. The change in SEE was not significantly correlated with the change in SM or liver mass, nor with blood analyses; however, a significant relationship between the change in kidney mass and SEE was observed. Conclusions: Based on our results, there is a possibility that the mass of the kidneys has an effect on the change in SEE following weight change in college Sumo wrestlers.
Application of Hybrid Membrane Processes Coupling Separation and Biological or Chemical Reaction in Advanced Wastewater Treatment
Authors: Molinari Raffaele , Lavorato Cristina , Argurio Pietro
Published: October 2020

The rapid urbanization and water shortage impose an urgent need in improving sustainable water management without compromising the socioeconomic development all around the world. In this context, reclaimed wastewater has been recognized as a sustainable water management strategy since it represents an alternative water resource for non-potable or (indirect) potable use. The conventional wastewater remediation approaches for the removal of different emerging contaminants (pharmaceuticals, dyes, metal ions, etc.) are unable to remove/destroy them completely. Hybrid membrane processes (HMPs) are a powerful solution for removing emerging pollutants from wastewater. On this aspect, the present paper focused on HMPs obtained by the synergic coupling of biological and/or chemical reaction driven processes with membrane processes, giving a critical overview and particular emphasis on some case studies reported in the pertinent literature. By using these processes, a satisfactory quality of treated water can be achieved, permitting its sustainable reuse in the hydrologic cycle while minimizing environmental and economic impact.
Ultrasonic Retinal Neuromodulation and Acoustic Retinal Prosthesis
Authors: Lo Pei-An , Huang Kyana , Zhou Qifa , Humayun Mark S. , Yue Lan
Published: October 2020

Ultrasound is an emerging method for non-invasive neuromodulation. Studies in the past have demonstrated that ultrasound can reversibly activate and inhibit neural activities in the brain. Recent research shows the possibility of using ultrasound ranging from 0.5 to 43 MHz in acoustic frequency to activate the retinal neurons without causing detectable damages to the cells. This review recapitulates pilot studies that explored retinal responses to the ultrasound exposure, discusses the advantages and limitations of the ultrasonic stimulation, and offers an overview of engineering perspectives in developing an acoustic retinal prosthesis. For comparison, this article also presents studies in the ultrasonic stimulation of the visual cortex. Despite that, the summarized research is still in an early stage; ultrasonic retinal stimulation appears to be a viable technology that exhibits enormous therapeutic potential for non-invasive vision restoration.
Acanthamoeba castellanii as a Screening Tool for Mycobacterium avium Subspecies paratuberculosis Virulence Factors with Relevance in Macrophage Infection
Authors: Phillips Ida L. , Everman Jamie L. , Bermudez Luiz E. , Danelishvili Lia
Published: October 2020

The high prevalence of Johne’s disease has driven a continuous effort to more readily understand the pathogenesis of the etiological causative bacterium, Mycobacterium avium subsp. paratuberculosis (MAP), and to develop effective preventative measures for infection spread. In this study, we aimed to create an in vivo MAP infection model employing an environmental protozoan host and used it as a tool for selection of bacterial virulence determinants potentially contributing to MAP survival in mammalian host macrophages. We utilized Acanthamoeba castellanii (amoeba) to explore metabolic consequences of the MAP-host interaction and established a correlation between metabolic changes of this phagocytic host and MAP virulence. Using the library of gene knockout mutants, we identified MAP clones that can either enhance or inhibit amoeba metabolism and we discovered that, for most part, it mirrors the pattern of MAP attenuation or survival during infection of macrophages. It was found that MAP mutants that induced an increase in amoeba metabolism were defective in intracellular growth in macrophages. However, MAP clones that exhibited low metabolic alteration in amoeba were able to survive at a greater rate within mammalian cells, highlighting importance of both category of genes in bacterial pathogenesis. Sequencing of MAP mutants has identified several virulence factors previously shown to have a biological relevance in mycobacterial survival and intracellular growth in phagocytic cells. In addition, we uncovered new genetic determinants potentially contributing to MAP pathogenicity. Results of this study support the use of the amoeba model system as a quick initial screening tool for selection of virulence factors of extremely slow-grower MAP that is challenging to study.
Legacy Effects Overshadow Tree Diversity Effects on Soil Fungal Communities in Oil Palm-Enrichment Plantations
Authors: Ballauff Johannes , Zemp Delphine Clara , Schneider Dominik , Irawan Bambang , Daniel Rolf , Polle Andrea
Published: October 2020

Financially profitable large-scale cultivation of oil palm monocultures in previously diverse tropical rain forest areas constitutes a major ecological crisis today. Not only is a large proportion of the aboveground diversity lost, but the belowground soil microbiome, which is important for the sustainability of soil function, is massively altered. Intermixing oil palms with native tree species promotes vegetation biodiversity and stand structural complexity in plantations, but the impact on soil fungi remains unknown. Here, we analyzed the diversity and community composition of soil fungi three years after tree diversity enrichment in an oil palm plantation in Sumatra (Indonesia). We tested the effects of tree diversity, stand structural complexity indices, and soil abiotic conditions on the diversity and community composition of soil fungi. We hypothesized that the enrichment experiment alters the taxonomic and functional community composition, promoting soil fungal diversity. Fungal community composition was affected by soil abiotic conditions (pH, N, and P), but not by tree diversity and stand structural complexity indices. These results suggest that intensive land use and abiotic filters are a legacy to fungal communities, overshadowing the structuring effects of the vegetation, at least in the initial years after enrichment plantings.
The Core and Distinction of the Gut Microbiota in Chinese Populations across Geography and Ethnicity
Authors: Lin Deng , Wang Ran , Luo Junjie , Ren Fazheng , Gu Zhenglong , Zhao Yiqiang , Zhao Liang
Published: October 2020

The diversity of the human gut microbiota constitutes a fundamental health indicator of different populations. The relative importance of geographical location and ethnicity on the gut microbiota, however, has not been previously addressed. Due to unique ethnic distributions across China, we recruited distinct minority ethnic groups, including Han populations, in each of the seven cities that were explored in this study. We investigated the gut microbiota of 394 healthy subjects (14 groups) from these seven different cities using 16S rRNA sequencing. Our results indicated that both geographical location and ethnicity were major factors. However, geographical location exhibited greater influence than ethnicity on both the composition and diversity of the gut microbiota. In addition, a total of 15 shared biomarkers at the genus level were identified in three distinct locations, including seven in Inner Mongolia, seven in Xinjiang and one in Gansu. Furthermore, 65 unique biomarkers were found in 14 population groups, which indicated specific communities in different populations. Based on the gut microbiota species, two main enterotypes—namely Prevotella (ETP) and Bacteroides (ETB), which consist of Prevotella and Bacteroides as the core bacterial genus, were observed in Chinese populations. Our unique experimental design using the same ethnic group—Han, as a control in different locations, enables delineating the importance of geographical location and ethnicity on the gut microbiota, and provides the fundamental characteristics of gut microbiota diversity in Chinese populations.
Selective Removal of As(V) Ions from Acid Mine Drainage Using Polymer Inclusion Membranes
Authors: Zawierucha Iwona , Nowik-Zajac Anna , Malina Grzegorz
Published: October 2020

Acid mine drainage (AMD) is globally recognized as one of the environmental pollutants of the priority concern due to high concentrations of toxic metals and sulfates. More rigorous environmental legislation requires exploitation of effective technologies to remove toxic metals from contaminated streams. In view of high selectivity, effectiveness, durability, and low energy demands, the separation of toxic metal ions using immobilized membranes with admixed extractants could ameliorate water quality. Cellulose triacetate based polymer inclusion membranes (PIMs), with extractant and plasticizer, were studied for their ability to transport of As(V) ions from synthetic aqueous leachates. The effects of the type and concentration of extractant, plasticizer content, and sulfuric acid concentration in source phase on the arsenic removal efficiency have been assessed. Under the best of applied conditions, PIM with Cyanex 921 as extractant and o-nitrophenyl octyl ether (o-NPOE) as plasticizer showed high repeatability and excellent transport activity for selective removal of As(V) from AMD.
Opportunities for Persistent Luminescent Nanoparticles in Luminescence Imaging of Biological Systems and Photodynamic Therapy
Authors: Fritzen Douglas L. , Giordano Luidgi , Rodrigues Lucas C. V. , Monteiro Jorge H. S. K.
Published: October 2020

The use of luminescence in biological systems allows us to diagnose diseases and understand cellular processes. Persistent luminescent materials have emerged as an attractive system for application in luminescence imaging of biological systems; the afterglow emission grants background-free luminescence imaging, there is no need for continuous excitation to avoid tissue and cell damage due to the continuous light exposure, and they also circumvent the depth penetration issue caused by excitation in the UV-Vis. This review aims to provide a background in luminescence imaging of biological systems, persistent luminescence, and synthetic methods for obtaining persistent luminescent materials, and discuss selected examples of recent literature on the applications of persistent luminescent materials in luminescence imaging of biological systems and photodynamic therapy. Finally, the challenges and future directions, pointing to the development of compounds capable of executing multiple functions and light in regions where tissues and cells have low absorption, will be discussed.
Graphene Domain Signature of Raman Spectra of sp2 Amorphous Carbons
Authors: Sheka Elena F. , Golubev Yevgeny A. , Popova Nadezhda A.
Published: October 2020

The standard D-G-2D pattern of Raman spectra of sp2 amorphous carbons is considered from the viewpoint of graphene domains presenting their basic structure units (BSUs) in terms of molecular spectroscopy. The molecular approximation allows connecting the characteristic D-G doublet spectra image of one-phonon spectra with a considerable dispersion of the C=C bond lengths within graphene domains, governed by size, heteroatom necklace of BSUs as well as BSUs packing. The interpretation of 2D two-phonon spectra reveals a particular role of electrical anharmonicity in the spectra formation and attributes this effect to a high degree of the electron density delocalization in graphene domains. A size-stimulated transition from molecular to quasi-particle phonon consideration of Raman spectra was experimentally traced, which allowed evaluation of a free path of optical phonons in graphene crystal.
From Nanobiotechnology, Positively Charged Biomimetic Dendrimers as Novel Antibacterial Agents: A Review
Authors: Alfei Silvana , Schito Anna Maria
Published: October 2020

The alarming increase in antimicrobial resistance, based on the built-in abilities of bacteria to nullify the activity of current antibiotics, leaves a growing number of bacterial infections untreatable. An appealing approach, advanced in recent decades, concerns the development of novel agents able to interact with the external layers of bacteria, causing irreparable damage. Regarding this, some natural cationic antimicrobial peptides (CAMPs) have been reconsidered, and synthetic cationic polymers, mimicking CAMPs and able to kill bacteria by non-specific detrimental interaction with the negative bacterial membranes, have been proposed as promising solutions. Lately, also dendrimers were considered suitable macromolecules for the preparation of more advanced cationic biomimetic nanoparticles, able to harmonize the typical properties of dendrimers, including nanosize, mono-dispersion, long-term stability, high functionality, and the non-specific mechanism of action of CAMPs. Although cationic dendrimers are extensively applied in nanomedicine for drug or gene delivery, their application as antimicrobial agents is still in its infancy. The state of the art of their potential applications in this important field has therefore been reviewed here, with particular attention to the innovative case studies in the literature including also amino acid-modified polyester-based dendrimers, practically unexplored as membrane-active antimicrobials and able to kill bacteria on contact.
The Association between Nutritional Status and In-Hospital Mortality among Patients with Acute Coronary Syndrome—A Result of the Retrospective Nutritional Status Heart Study (NSHS)
Authors: Czapla Michał , Karniej Piotr , Juárez-Vela Raúl , Łokieć Katarzyna
Published: October 2020

Background: Nutritional status is related to the prognosis and the length of hospitalization of individuals with myocardial infarction. This study aimed to assess the effects of nutritional status on in-hospital mortality in patients with acute coronary syndrome. Methods: We performed a retrospective study of 1623 medical records of patients admitted to the cardiology department of the University Clinical Hospital in Wroclaw (Poland) between 2017 and 2019. Results: It was found that, of those who died in the sample, 50% had a BMI within the normal range, 29% were in the overweight range and 18% were in the obese range. Patients who died had significantly more frequent occurrences of the following: Nutrition Risk Screening (NRS) ≥ 3 (20% vs. 6%; p < 0.001); heart failure (53% vs. 25%; p < 0.001); or a history of stroke (22% vs. 9%; p < 0.001), arterial hypertension (66% vs. 19%; p < 0001) or diabetes (41% vs. 19%; p < 0.001). Statistically significant differences were found when considering the type of infarction, diabetes or people with low-density lipoprotein greater than or equal to 70 mg/dL. Conclusions: This study shows that malnutrition correlates with an increased risk of death during hospitalization.
Maternal Arsenic Exposure and Gestational Diabetes: A Systematic Review and Meta-Analysis
Authors: Salmeri Noemi , Villanacci Roberta , Ottolina Jessica , Bartiromo Ludovica , Cavoretto Paolo , Dolci Carolina , Lembo Rosalba , Schimberni Matteo , Valsecchi Luca , Viganò Paola , Candiani Massimo
Published: October 2020

Gestational diabetes mellitus (GDM) is a metabolic complication associated with adverse outcomes for mother and fetus. Arsenic (As) exposure has been suggested as a possible risk factor for its development. The aim of this meta-analysis was to provide a comprehensive overview of published evidence on the association between As and GDM. The systematic search from PubMed, MEDLINE, and Scopus was limited to full-length manuscripts published in peer-reviewed journals up to April 2020, identifying fifty articles. Ten studies met the inclusion criteria, nine for quantitative synthesis with a total of n = 1984 GDM cases. The overall pooled risk was 1.56 (95% Confidence Interval - CI = 1.23, 1.99) with moderate heterogeneity (χ2 = 21.95; I2% = 64). Several differences among the included studies that may account for heterogeneity were investigated. Stratification for exposure indicator confirmed a positive association for studies assessing urine As. A slightly higher risk was detected pooling studies based in Asia rather than in North America. Stratification for GDM diagnostic criteria showed higher risks when diagnosis was made according to the Canadian Diabetes Association (CDA-SOGC) or World Health Organization (WHO) criteria, whereas a lower risk was observed when adopting the American Diabetes Association (ADA) criteria. These results provide additional evidence for a possible association between As exposure and GDM, although the data need to be interpreted with caution due to heterogeneity.
Quercetin Intervention Alleviates Offspring’s Oxidative Stress, Inflammation, and Tight Junction Damage in the Colon Induced by Maternal Fine Particulate Matter (PM2.5) Exposure through the Reduction of Bacteroides
Authors: Liu Wei , Zhou Yalin , Qin Yong , Yu Lanlan , Li Ruijun , Chen Yuhan , Xu Yajun
Published: October 2020

The influences of maternal fine particulate matter (PM2.5) exposure on intestinal oxidative stress, inflammation, tight junctions, and gut microbiota of offspring are not well understood. Moreover, research on the dietary intervention method has not been well studied. In our study, dams received PM2.5 and quercetin intervention during gestation and lactation, and then inflammation biomarkers, oxidative stress indicators, tight junction proteins, and gut microbiota in the colon of offspring were analyzed. Compared with the control group, lower catalase (CAT) and superoxide dismutase (SOD) activities, higher interleukin-17A (IL-17A) and interleukin-22 (IL-22), decreased ZO-1 and occludin expressions, and higher Bacteroides abundance were observed in the offspring mice of the PM2.5 group. However, higher CAT and SOD activities, lower IL-17A and IL-22 levels, increased ZO-1 and occludin expressions, and lower Bacteroides abundance were found in the quercetin groups. In addition, there was a negative correlation between Bacteroides abundance and CAT concentration. Additionally, Bacteroides abundance was positively related to IL-17A and IL-22 levels. These findings suggest that maternal PM2.5 exposure may have some certain effects on intestinal oxidative stress, inflammation, and tight junctions. Quercetin administration may protect the offspring against these adverse effects. Changes of Bacteroides abundance play an important role in the process.
Skeletal Muscle Loss during Multikinase Inhibitors Therapy: Molecular Pathways, Clinical Implications, and Nutritional Challenges
Authors: Rinninella Emanuele , Cintoni Marco , Raoul Pauline , Pozzo Carmelo , Strippoli Antonia , Ponziani Francesca Romana , Pompili Maurizio , Bria Emilio , Tortora Giampaolo , Gasbarrini Antonio , Mele Maria Cristina
Published: October 2020

In cancer patients, loss of muscle mass is significantly associated with low tolerability of chemotherapy and poor survival. Despite the great strides in the treatment of cancer, targeted therapies such as tyrosine kinase inhibitors (TKIs) could exacerbate muscle wasting. Over recent years, the impact of skeletal muscle loss during TKI therapy on clinical outcomes has been in the spotlight. In this review, we focus on the different molecular pathways of TKIs potentially involved in muscle wasting. Then, we report the results of the studies assessing the effects of different TKI therapies—such as sorafenib, regorafenib, sunitinib, and lenvatinib—on muscle mass, and highlight their potential clinical implications. Finally, we discuss an integrative nutritional approach to be adopted during TKI treatment. The assessment of muscle mass from computerized tomography imaging could be helpful in predicting toxicity and prognosis in patients treated with TKI such as sorafenib. Early recognition of low muscle mass and effective personalized nutritional support could prevent or attenuate muscle mass wasting. However, the role of nutrition is still overlooked, and future clinical trials are needed to find the optimal nutritional support to countermeasure muscle mass depletion during TKI therapy.
Effect of Fat-Soluble Vitamins A, D, E and K on Vitamin Status and Metabolic Profile in Patients with Fat Malabsorption with and without Urolithiasis
Authors: Siener Roswitha , Machaka Ihsan , Alteheld Birgit , Bitterlich Norman , Metzner Christine
Published: October 2020

Patients with intestinal fat malabsorption and urolithiasis are particularly at risk of acquiring fat-soluble vitamin deficiencies. The aim of the study was to evaluate the vitamin status and metabolic profile before and after the supplementation of fat-soluble vitamins A, D, E and K (ADEK) in 51 patients with fat malabsorption due to different intestinal diseases both with and without urolithiasis. Anthropometric, clinical, blood and 24-h urinary parameters and dietary intake were assessed at baseline and after ADEK supplementation for two weeks. At baseline, serum aspartate aminotransferase (AST) activity was higher in stone formers (SF; n = 10) than in non-stone formers (NSF; n = 41) but decreased significantly in SF patients after supplementation. Plasma vitamin D and E concentrations increased significantly and to a similar extent in both groups during intervention. While plasma vitamin D concentrations did not differ between the groups, vitamin E concentrations were significantly lower in the SF group than the NSF group before and after ADEK supplementation. Although vitamin D concentration increased significantly in both groups, urinary calcium excretion was not affected by ADEK supplementation. The decline in plasma AST activity in patients with urolithiasis might be attributed to the supplementation of ADEK. Patients with fat malabsorption may benefit from the supplementation of fat-soluble vitamins ADEK.
Efficient Enrichment of Retinal DHA with Dietary Lysophosphatidylcholine-DHA: Potential Application for Retinopathies
Authors: Sugasini Dhavamani , Yalagala Poorna , Subbaiah Papasani
Published: October 2020

Although decreased retinal docosahexaenoic acid (DHA) is a known risk factor for retinopathy, currently available omega-3 fatty acid supplements, which are absorbed as triacylglycerol (TAG), do not significantly enrich retinal DHA. We tested the hypothesis that lysophospahtidylcholine (LPC)-DHA which is absorbed as phospholipid, would efficiently increase retinal DHA because of the presence of LPC-specific transporter at the blood–retina barrier. In normal rats, LPC-DHA and di-DHA phosphatidylcholine (PC), which generates LPC-DHA during digestion, increased retinal DHA by 101% and 45%, respectively, but TAG-DHA had no significant effect at the same dose (40 mg/kg, 30 days). In normal mice, both sn-1 DHA LPC and sn-2 DHA LPC increased retinal DHA by 80%, but free DHA had no effect. Lipase-treated krill oil (which contains LPC-DHA and LPC-EPA (eicosapentaenoic acid), but not normal krill oil (which has little LPC), increased both retinal DHA (+76%) and EPA (100-fold). Fish oil, however, had no effect, whether lipase-treated or not. These studies show that retinal DHA can be efficiently increased by dietary LPC-DHA, but not by TAG-DHA or free DHA. Since DHA is known to be protective against retinopathy and other eye diseases, this study provides a novel nutraceutical approach for the prevention/treatment of these diseases.
Inducible Nitric Oxide Regulates Na-Glucose Co-Transport in a Spontaneous SAMP1/YitFc Mouse Model of Chronic Ileitis
Authors: Palaniappan Balasubramanian , Sundaram Shanmuga , Arthur Subha , Afroz Sheuli , Sundaram Uma
Published: October 2020

In mammalian small intestine, glucose is primarily absorbed via Na-dependent glucose co-transporter (SGLT1) on the brush border membrane (BBM) of absorptive villus cells. Malabsorption of nutrients (e.g., glucose) leads to malnutrition, a common symptom of inflammatory bowel disease (IBD), where the mucosa is characterized by chronic inflammation. Inducible nitric oxide (iNO) is known to be elevated in IBD mucosa. SAMP1/YitFc (SAMP1) mouse is a spontaneous model of chronic ileitis that develops lesions in its terminal ileum, very similar to human IBD. How SGLT1 may be affected in SAMP1 model of chronic ileitis is unknown. Ten-week-old SAMP1 mice with AKR mice as control were treated with N6-(1-iminoethyl)-L-lysine dihydrochloride (L-NIL) to inhibit iNO production. Intracellular NO levels were found to be increased in villus cells from SAMP1 mice. Moreover, SGLT1 and Na+/K+-ATPase activities and BBM SGLT1 expression were significantly decreased. However, L-NIL treatment reduced the intracellular iNO production, and reversed both downregulated SGLT1 and Na+/K+-ATPase activities in SAMP1 mice. Inhibition of iNO by L-NIL treatment also significantly reversed the BBM SGLT1 protein expression in SAMP1 mice. L-NIL reversed the inflammation mediated downregulation of SGLT1 activity by restoring the BBM SGLT1 expression. Thus, regulation of SGLT1 in chronic ileitis is likely mediated by iNO.
Socio-Demographic Characteristics and Body Weight Perceptions of Study Participants Benefitting Most from the Feel4Diabetes Program Based on Their Anthropometric and Glycaemic Profile Changes
Authors: Moschonis George , Karatzi Kalliopi , Apergi Kyriaki , Liatis Stavros , Kivelä Jemina , Wikström Katja , Ayala-Marín Alelí M. , Mateo-Gallego Rocio , Tsochev Kaloyan , Chakarova Nevena , Antal Emese , Rurik Imre , Iotova Violeta , Cardon Greet , Lindstrom Jaana , Moreno Luis A. , Makrilakis Konstantinos , Manios Yannis
Published: October 2020

The Feel4Diabetes program was comprised of a community-based screening and a two-year intervention phase aiming to prevent type 2 diabetes (T2D) in families at risk for diabetes across Europe. The current work aimed to identify the socio-demographic characteristics and body weight perceptions of participants who benefitted the most, achieving at least a 5% reduction in body weight, waist circumference and glycaemic indices (fasting plasma glucose, insulin, glycosylated haemoglobin levels), over two-year period. Following a two-stage screening procedure, 2294 high-risk parents were randomly allocated to standard care or more intensive intervention. The participants who benefitted most were living in Southern (OR 2.39–3.67, p < 0.001) and Eastern Europe (OR 1.55–2.47, p < 0.05), received more intensive intervention (OR 1.53–1.90, p = 0.002) and were younger (<40 years old) adults (OR 1.48–1.51, p < 0.05). Furthermore, individuals with tertiary education (OR 2.06, p < 0.001), who were unemployed (OR 1.62–1.68, p < 0.05) and perceived their body weight to be higher than normal (OR 1.58–3.00, p < 0.05) were more likely to benefit from the program. Lastly, males were more likely to show improvements in their glycaemic profiles compared to females (OR 1.40, p = 0.024). These findings point out the regions in Europe and the sociodemographic profile of individuals that benefitted the most in the current study, highlighting the need to prioritise regions in greater need for such interventions and also tailor future interventions to the characteristics and perceptions of the target populations.
Associations between Changes in Health Behaviours and Body Weight during the COVID-19 Quarantine in Lithuania: The Lithuanian COVIDiet Study
Authors: Kriaucioniene Vilma , Bagdonaviciene Lina , Rodríguez-Pérez Celia , Petkeviciene Janina
Published: October 2020

The COVID-19 quarantine has caused significant changes in everyday life. This study aimed to evaluate the effect of the quarantine on dietary, physical activity and alcohol consumption habits of Lithuanians and the association between health behaviours and weight changes. An online cross-sectional survey was carried out among individuals older than 18 years in April 2020. The self-administered questionnaire included health behaviour and weight change data. Altogether 2447 subjects participated in the survey. Almost half of the respondents (49.4%) ate more than usual, 45.1% increased snacking, and 62.1% cooked at home more often. Intake of carbonated or sugary drinks, fast food and commercial pastries decreased, while consumption of homemade pastries and fried food increased. A decrease in physical activity was reported by 60.6% of respondents. Every third (31.5%) respondent, more often those already with overweight, gained weight. Multivariate logistic regression analysis showed that the higher odds of weight gain were associated with females, older age, increased consumption of sugary drinks, homemade pastries and fried food, eating more than usual, increased snacking, decreased physical activity and increased alcohol consumption. Our data highlighted the need for dietary and physical activity guidelines to prevent weight gain during the period of self-isolation, especially targeting those with overweight and obesity.
Eurycoma longifolia—Infused Coffee—An Oral Toxicity Study
Authors: Ahmad Norzahirah , Teh Bee Ping , Halim Siti Zaleha , Zolkifli Nor Azlina , Ramli Nurulfariza , Muhammad Hussin
Published: October 2020

Coffee infused with the additive Eurycoma longifolia, also known as Tongkat ali (TA), has become widely available in the Malaysian market. Safety evaluations for consumption of the products have been called for due to the herbal addition. This study investigates the acute, subacute and chronic effects of a commercial TA coffee in Sprague Dawley rats when given in a single, repeated and prolonged dosage. The dosages of 0.005, 0.05, 0.30 and 2 g/kg body weight (BW) were used in the acute study and 0.14, 0.29 and 1 g/kg BW were used in the repeated dose studies. The in-life parameters measured were food and water intake, body weight and clinical observations. Blood were collected for hematology and clinical biochemistry analyses. All animals were subjected to full necropsies. Non-toxicity-related changes were observed in the food and water consumption parameters. Body weight showed normal increments and none of the animals had any clinical signs of toxicity. Microscopically assessed organ tissues did not reveal any abnormalities. There was significant decrease of platelet count in all the chronic study male treated groups. Significant elevation of renal profile parameters in both gender groups given 0.29 g/kg BW, along with liver and lipid profile elevation in some female groups of the chronic study were noted. No dose-dependent relationship was apparent in the dosage range tested, though these changes may suggest an initial safety indication to the TA coffee. The study concludes that the no observed adverse effect level (NOAEL) for this commercial TA coffee was 1 g/kg BW.
A Randomized, Double-Blinded, Placebo-Controlled, Clinical Study of the Effects of a Nutraceutical Combination (LEVELIP DUO®) on LDL Cholesterol Levels and Lipid Pattern in Subjects with Sub-Optimal Blood Cholesterol Levels (NATCOL Study)
Authors: Cicero Arrigo F.G. , D’Addato Sergio , Borghi Claudio
Published: October 2020

Phytosterols and red yeast rice are largely studied cholesterol-lowering nutraceuticals, respectively inhibiting the bowel absorption and liver synthesis of cholesterol. Our aim was to test the effect of combined nutraceutical-containing phytosterols and red yeast rice vs. a placebo on the lipid profile. We performed a parallel arms, double-blind, placebo-controlled clinical trial, randomizing 88 moderately hypercholesterolemic subjects to treatment with a combined nutraceutical containing phytosterols (800 mg) and red yeast rice, standardized to contain 5 mg of monacolins from Monascus purpureus, with added niacin (27 mg) and policosanols (10 mg) (LEVELIP DUO®), or placebo. The mean LDL-Cholesterol (LDL-C) change at Week 8 was −32.5 ± 30.2 mg/dL (−19.8%) in the combined nutraceutical group and 2.5 ± 19.4 mg/dL (2.3%) in the placebo group. The estimated between-group difference of −39.2 mg/dL (95% CI: −48.6; −29.8) indicates a statistically significant difference between treatments in favor of the combined nutraceutical (p < 0.0001). Total Cholesterol (TC), non-HDL cholesterol (non-HDL-C), Apolipoprotein B, TC/HDL-C and LDL-C/HDL-C improved in a similar way in the combined nutraceutical group only. No significant changes in other clinical and laboratory parameters were observed. In conclusion, the tested combined nutraceutical was well tolerated, while significantly reducing the plasma levels of LDL-C, TC, non-HDL-C, ApoB, TC/HDL-C and LDL-C/HDL-C ratios in mildly hypercholesterolemic patients. Trial registration (ClinicalTrials.gov): NCT03739242.
Clinical Characteristics and Outcomes of S. Aureus Bacteremia in Patients Receiving Total Parenteral Nutrition
Authors: Gompelman Michelle , Tuinte Renée A. M. , Berrevoets Marvin A. H. , Bleeker-Rovers Chantal P. , Wanten Geert J. A.
Published: October 2020

Background: Patients on total parenteral nutrition (TPN) are at risk of developing central line-associated infections. Specifically, Staphylococcus aureus bacteremia (SAB) is feared for its high complication rates. This prospective cohort study compares characteristics, clinical course and outcome of SAB in patients with and without TPN support. Methods: Clinical and microbiological data from all patients with positive blood cultures for S. aureus from two facilities, including our referral center for TPN support, were retrieved (period 2013–2020). Primary outcome was overall mortality, and included survival analysis using a multivariate Cox regression model. Secondary outcomes comprised a comparison of clinical characteristics and outcomes between both patient groups and analysis of factors associated with complicated outcome (e.g., endocarditis, deep-seated foci, relapse and death) in patients on TPN specifically. Results: A total of 620 SAB cases were analyzed, of which 53 cases received TPN at the moment the blood culture was taken. Patients in the TPN group were more frequently female, younger and had less comorbidity (p < 0.001). In-hospital death and overall mortality were significantly lower in TPN patients (4% vs. 18%, p = 0.004 and 10% vs. 34%, p < 0.001, respectively). Positive follow-up blood cultures, delayed onset of therapy and previous catheter problems were associated with a higher incidence of complicated SAB outcome in patients on TPN. Conclusion: Our data show that patients on TPN have a milder course of SAB with lower mortality rates compared to non-TPN SAB patients.
Sweet Taste Antagonist Lactisole Administered in Combination with Sucrose, But Not Glucose, Increases Energy Intake and Decreases Peripheral Serotonin in Male Subjects
Authors: Schweiger Kerstin , Grüneis Verena , Treml Julia , Galassi Claudia , Karl Corinna M. , Ley Jakob P. , Krammer Gerhard E. , Lieder Barbara , Somoza Veronika
Published: October 2020

Knowledge regarding the involvement of sweetness perception on energy intake is scarce. Here, the impact of glucose and sucrose sweetness, beyond their caloric load, on subsequent food intake and biomarkers of satiation was evaluated by co-administration of the sweet taste receptor inhibitor lactisole. A total of 27 healthy, male subjects received solutions of either 10% glucose w/o 60 ppm lactisole or 10% sucrose w/o 60 ppm lactisole. Subsequent food intake from a standardized breakfast was evaluated 2 h after receiving the respective test solution. Changes in postprandial plasma concentrations of cholecystokinin, ghrelin, and serotonin were determined over a period of 120 min, as was the body temperature. Administration of lactisole to the sucrose solution increased the energy intake from the subsequent standardized breakfast by 12.9 ± 5.8% (p = 0.04), led to a decreased Δ AUC of the body core temperature by 46 ± 20% (p = 0.01), and time-dependently reduced Δ serotonin plasma concentrations (−16.9 ± 6.06 ng/mL vs. −0.56 ± 3.7 ng/mL after sucrose administration, p = 0.03). The present study shows that lactisole increases energy intake and decreases plasma serotonin concentrations as well as body core temperature induced by sucrose, but not glucose. This finding may be associated with the different binding affinities of sucrose and glucose to the sweet taste receptor.
Equation of State of Strongly Magnetized Matter with Hyperons and Δ-Resonances
Authors: Thapa Vivek Baruah , Sinha Monika , Li Jia Jie , Sedrakian Armen
Published: October 2020

We construct a new equation of state for the baryonic matter under an intense magnetic field within the framework of covariant density functional theory. The composition of matter includes hyperons as well as Δ-resonances. The extension of the nucleonic functional to the hypernuclear sector is constrained by the experimental data on Λ and Ξ-hypernuclei. We find that the equation of state stiffens with the inclusion of the magnetic field, which increases the maximum mass of neutron star compared to the non-magnetic case. In addition, the strangeness fraction in the matter is enhanced. Several observables, like the Dirac effective mass, particle abundances, etc. show typical oscillatory behavior as a function of the magnetic field and/or density which is traced back to the occupation pattern of Landau levels.