bluuebunny/crossref_metadata_2025 huggingface dataset:
Significant Shifts in Predominant Plant Dispersal Modes in Pine Forests of the Southern Urals (Russia): Responses to Technogenic Pollution and Ground Fires
Authors: Veselkin Denis , Kuyantseva Nadezhda , Mumber Aleksandr , Zharkova Darya
Published: December 2024

The purpose of this work was to assess the functional diversity of herb–shrub layer com munities determined by their dispersal mode in pine boreal forests depending on two factors: (i) the degree of technogenic heavy metal pollution and (ii) the time passed since the last fire. We tested two hypotheses: (1) the functional diversity of communities determined by their diaspore dispersal mode decreases in polluted forests and in forests disturbed by recent fires; (2) the abundance, i.e., participation of anemochorous species in communities, is relatively greater in polluted forests and in forests disturbed by recent fires than in unpolluted or in forests that have not burned for a long time. We analyzed 77 vegetation relevés made in polluted and unpolluted pine forests to obtain the impact gradient of the Karabash copper smelter (South Urals, Russia). The studied forests also had different durations of time since the last ground fire—from 1 to 60 years. Two classifications of the diaspore dispersal modes were used. We found that community functional diversity and predominant dispersal modes changed significantly in response to technogenic pollution and, to a lesser extent, in response to ground fires. In polluted forests, the importance of species with a long diaspore dispersal distance—anemochores and zoochores—increased. This result suggests conducting a specific study of long-distance diaspore migration as a possibly underestimated factor of community formation under severe technogenic disturbances. The importance of zoochores in a broad sense, including species with diaspores dispersed by vertebrates and invertebrates, increased in post-fire succession. This result coincides with the known pattern of increasing abundance of zoochorous plants in regenerative successions in tropical forests. Therefore, the data on plant–animal interactions can possibly provide valuable information on succession mechanisms in taiga forests.
Prospects for Oak Cultivation in Europe Under Changing Environmental Conditions and Increasing Pressure from Harmful Organisms
Authors: Lyubenova Aneta , Baranowska Marlena , Menkis Audrius , Davydenko Kateryna , Nowakowska Justyna , Borowik Piotr , Oszako Tomasz
Published: December 2024

It is assumed that climate change (global warming) worsens the living conditions for conifers and at the same time favours the cultivation of deciduous trees, including oaks. In fact, in Poland, for example, many more oaks are now being planted as forest-forming tree species than in the 1980s and 1990s. However, the monitoring of the health status of European forests (according to the International Co-operation Project) does not confirm these optimistic assumptions, and oak has been cited as one of the most damaged tree species in terms of defoliation in recent decades. The prospects for oak cultivation in European forestry are therefore a combination of abiotic conditions and biotic damage factors. This review article focuses in particular on the new threats posed by pathogenic organisms causing emerging diseases. These include newly identified bacteria responsible for the so-called Acute Oak Decline (AOD), oomycetes (especially those specialised in damaging fine roots, such as Phytophthora quercina T.Jung) and semi-parasites of the genus Loranthus. At the same time, the pressure from commonly observed insects and fungi described in connection with the complex syndrome of oak decline, which is divided into predisposing, inciting, and contributing factors (according to Manion’s disease spiral), has not abated. Therefore, international, interdisciplinary research (such as that proposed in Oakland) is needed, using modern technologies (RS remote sensing) based on the comparison of satellite images (from different years), not only to inventory the most valuable oak stands in Europe (microrefugia) but also to identify trends in changes in their condition and biodiversity. As RS has its limitations (e.g., resolution), aerial monitoring should be complemented by quantitative and qualitative inventory from the ground, e.g., monitoring of the presence of soil microorganisms using effective molecular biological methods (e.g., Next-Generation Sequencing NGS).
Responses of Soil Infiltration and Erodibility to Vegetation Succession Stages at Erosion and Deposition Sites in Karst Trough Valleys
Authors: Shi Hailong , Gan Fengling , Jiang Lisha , Tan Xiaohong , Liu Dinghui , Yan Youjin , Fan Yuchuan , Pu Junbing
Published: December 2024

The topographies of soil erosion and deposition are critical factors that significantly influence soil quality, subsequently impacting the erodibility of soils in karst regions. However, the investigation into the effects of erosion and deposition topographies on soil erodibility across different stages of vegetation succession in karst trough valleys is still at a preliminary stage. Therefore, three distinct topographic features (dip slopes, anti-dip slopes, and valley depressions) were selected at erosion (dip/anti-dip slope) and deposition sites (valley) to investigate the spatial heterogeneity of soil physicochemical properties, infiltration capacity, aggregate stability, and erodibility in karst trough valleys. Additionally, five different stages of vegetation succession in karst forests were considered: Abandoned land stage (ALS), Herb stage (HS), Herb-Shrub stage (HES), Shrub stage (SHS), and Forest stage (FS). Additionally, the relationships among these factors were analyzed to identify the key driving factors influencing soil erodibility. The results revealed that soil physicochemical properties and soil aggregate stability at the deposition site were significantly superior to those at the erosion site. The FS resulted in the best soil physicochemical properties, whereas the HS resulted in the highest soil aggregate stability within the deposition site. However, the soil infiltration capacity at the erosion site was significantly greater than that at the deposition sites. The ALS had the strongest soil infiltration capacity at both the erosion and deposition sites. The soil erodibility at erosion sites (0.064) was significantly greater than that at deposition sites (0.051), with the highest soil erodibility observed on anti-dip slopes during the HES at erosion sites (0.142). The structural equation model reveals that erosion and deposition topographies, vegetation succession, soil physicochemical properties, soil aggregates, and soil infiltration characteristics collectively account for 88% of the variation in soil erodibility under different conditions. Specifically, both direct and indirect influences on soil erodibility are most significantly exerted by soil aggregate stability and vegetation succession. This study provides scientific evidence to support the management of soil erosion and ecological restoration in karst trough valleys while offering technical assistance for regional ecological improvement and poverty alleviation.
Enhancement of the Degradation of Phytosterol Side Chains in Mycolicibacterium by Eliminating the Redox Sensitivity of Key Thiolase and Augmenting Cell Activity
Authors: Zhou Xiuling , Liu Yuying , Li Fuyi , Huang Yang , Xuan Hongzhuan , Zhang Yang
Published: December 2024

Androstenedione (AD) is a vital intermediate in the synthesis of steroid drugs, making its efficient production critical in the steroid drug industry. Acetyl-CoA acetyltransferase (FadA5), a thiolase enzyme, plays an important role in the metabolic process of degrading phytosterol side chains in Mycolicibacterium to produce AD. This work is the first systematic analysis of the role of FadA5 in the transformation of phytosterols by Mycolicibacterium to produce AD. The relationship between redox potential and AD production was examined using resting cells, and it was confirmed that FadA5 is a key enzyme for AD production. Mutating the 87th cysteine of FadA5 to alanine reduced its redox effect, enhancing the substrate tolerance and biotransformation capacity of the strain. Co-expressing Vitreoscilla hemoglobin (VHb) and propionyl-CoA metabolized the transcription activator (PrpR), decreased intracellular reactive oxygen species levels, and improved cell viability. The AD yield of MSP-fA5C87A-VP/ΔfA5 was 2.541 g/L, an increase of 16.83% over the control strain. Using a repeated batch fermentation process, the production efficiency of the MSP-fA5C87A-VP/ΔfA5 strain was 0.658 g/L/d, which was 1.82 times higher than that of the control strain. These findings provide a theoretical basis for understanding and regulating steroid side-chain catabolism in Mycolicibacterium and offer support for the rational modification of industrial strains for steroidal drug precursor production.
Empowering LLMs with Toolkits: An Open-Source Intelligence Acquisition Method
Authors: Yuan Xinyang , Wang Jiarong , Zhao  Haozhi , Yan Tian , Qi Fazhi
Published: December 2024

The acquisition of cybersecurity threat intelligence is a critical task in the implementation of effective security defense strategies. Recently, advancements in large language model (LLM) technology have led to remarkable capabilities in natural language processing and understanding. In this paper, we introduce an LLM-based approach for open-source intelligence (OSINT) acquisition. This approach autonomously obtains OSINT based on user requirements, eliminating the need for manual scanning or querying, thus saving significant time and effort. To further address the knowledge limitations and timeliness challenges inherent in LLMs when handling threat intelligence, we propose a framework that integrates chain-of-thought techniques to assist LLMs in utilizing tools to acquire OSINT. Based on this framework, we have developed a threat intelligence acquisition agent capable of decomposing logical reasoning problems into multiple steps and gradually solving them using appropriate tools, along with a toolkit for the agent to dynamically access during the problem-solving process. To validate the effectiveness of our approach, we have designed four evaluation metrics to assess the agent’s performance and constructed a test set. Experimental results indicate that the agent achieves high accuracy rates in OSINT acquisition tasks, with a substantial improvement noted over its baseline large language model counterpart in specific intelligence acquisition scenarios.
Toward Classification of Fish Meat Using Fluorescence Excitation–Emission Matrix and Multivariate Statistics
Authors: Rahman Md. Mizanur , Shibata Mario , Rithu Mst. Nazira Akhter , Okazaki Emiko
Published: December 2024

Frequent intentional mislabeling of particular fish and fish products, such as the sale of frozen and thawed fish instead of fresh fish, occurs on all continents. Therefore, two studies were conducted to classify fish meat using excitation–emission matrix (EEM) nondestructively. The first study assessed EEM for differentiation between fresh and frozen–thawed spotted mackerel fillets. Fresh fillets were yielded with different post-mortem freshness variations (ice storage for 0–40 h). The right-side fillets were used as fresh fillets, whereas the left-side fillets were frozen and stored at −30 °C for three months, then thawed at 4 °C. Subsequently, EEM acquisition and chemical analyses were performed. Results of principal component analysis (PCA) of EEM spectra showed clear discrimination between fresh and frozen–thawed meat of fish fillet. In the second study, post-mortem freshness variations in four fish species (horse mackerel, spotted mackerel, cod, and flounder) were simulated by ice storage (0–48 h) and subsequent freezing. PCA of the EEM demonstrated a clear distinction among the fish meat categories, which was also revealed from the freshness data of chemical analysis. Results show that this novel method can be used to monitor fishery product authenticity.
Effects of Subacute Ammonia Nitrogen Stress on the Growth, Antioxidant Capability, and Immunity of Blunt Snout Bream (Megalobrama amblycephala) Juveniles
Authors: Yuan Xiangyang , Wang Qian , Dai Mengyang , Xiong Xinyu , Wang Hengjie , Wang Canli
Published: December 2024

To investigate the effects of subacute ammonia nitrogen stress on the growth, antioxidant capacity, and innate immunity of juvenile blunt snout bream (Megalobrama ambrycyphala), a sample of fish (average body weight 6.55 ± 0.24 g) was divided into three groups: a control group (G1), a 10% LC50 ammonia nitrogen stress group (G2), and a 20% LC50 ammonia nitrogen stress group (G3). The fish in G1 were reared in tap water with aeration. The fish in G2 were reared in water with 6.82 mg/L ammonium chloride at 10% LC50. The fish in G3 were reared in water with 13.64 mg/L ammonium chloride at 20% LC50. The feeding period was 4 weeks. Then, the growth, antioxidant, and immune response parameters of the fish were analyzed. The results showed that the median lethal concentration (LC50) of the ammonia nitrogen solution for blunt snout bream juveniles at 96 h was 68.18 mg/L. The final body weight, weight gain rate, specific growth rate (SGR), total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), albumin, globulin, acid phosphatase (ACP), and alkaline phosphatase (AKP) of fish in the stress groups were significantly lower than in the control group (p < 0.05); however, the MDA content of fish in the control group was significantly lower than that of those in the stress groups (p < 0.05). Furthermore, the relative expression levels of the GHRa, GHRb, IGF1, and IGF2 genes in the muscles of fish in the stress groups were significantly downregulated compared to the control group (p < 0.05). The relative expression levels of the SOD, CAT, Leap1, and Leap2 genes in the liver of fish in the stress groups were significantly lower than those in fish in the control group (p < 0.05). In conclusion, subacute ammonia nitrogen stress inhibited growth performance and decreased the antioxidant capacity and immunity of blunt snout bream.
The Evolution of Mechanical Properties and Cellular Structure of Apples During Freeze Drying Combined with Hot Air Drying (FD-HAD) Process
Authors: Li Lili , Yang Mengmeng , Zhu Lewen , Liu Wenchao , Li Linlin , Cao Weiwei , Chen Junliang , Zhao Linlin , Law Chung Lim , Yang Tongxiang , Ren Guangyue , Duan Xu
Published: December 2024

Apples are one of the most popular fruits in the world and have a significant share in domestic and international fruit production. Drying is a common method used to extend the shelf life of apples. However, it also induces irregular morphological changes in apples, which are essential to maintaining the structural integrity of the material. Therefore, it is necessary to understand the effect of cellular changes at the microscopic level on the macroscopic deformation of the material during drying. In this paper, the evolution of cell wall pectin fractions and viscoelastic properties of apples during freeze drying combined with hot air drying was investigated. The findings indicated that during the HAD stage, a decrease in the relaxation modulus (E1) of the samples was observed in the compression tests when the sample temperature was significantly higher than the glass transition temperature (Tg). As the difference between the two decreased, the samples exhibited increased stiffness and higher E1. The results of the pectin content analysis showed that the HAD process accelerated the loss and degradation of water-soluble pectin in the samples with high moisture content at the transition point. Simultaneously, the esterification degree of chelator-soluble pectin increased, leading to a reduction in the support provided to the cellular structure of the samples, which consequently affected their mechanical properties. These findings may provide valuable information for the application of freeze drying combined with hot air drying in the efficient processing of dried fruit and vegetable products.
Evaluation of Sensorial Markers in Deep-Fried Extra Virgin Olive Oils: First Report on the Role of Hydroxytyrosol and Its Derivatives
Authors: Mehany Taha , González-Sáiz José M. , Martínez Jorge , Pizarro Consuelo
Published: December 2024

Extra virgin olive oil (EVOO) is one of the main daily food items consumed around the world, particularly in the Mediterranean region, and it has unique organoleptic properties. This study aims to determine the best frying conditions of EVOO supplemented with natural exogenous antioxidants enriched in hydroxytyrosol (HTyr) and its derivatives from olive fruit extract (OFE) to conserve its positive sensorial attributes while minimizing its sensorial defects, in particular, rancidity under high thermal processes. In this study, an experienced panel assessed the sensory attributes of nine EVOO varieties, olive oil 0.4° (mixed virgin olive oil (VOO) with refined olive oil (ROO)), Orujo olive oil, and olive oil 1° (EVOO mixed with ROO), compared with two sunflower oil types, performed using a deep-frying (D-F) process with numerous variables, i.e., frying time, frying temperature, and the addition of polyphenols enriched with HTyr. Results showed that most EVOO samples were stable under D-F at 170 °C for 3 h, with added polyphenols (∼650 mg/kg). Moreover, at these best values, the results stated that Arbequina, Picual, Royuella, Hojiblanca, Arbosana, and Manzanilla oils have low rancidity scores with values of 0, 1.7, 1.8, 2.3, 3.1, and 3.7, respectively, and stable/higher positive sensorial attributes, i.e., fruity, bitter, and pungent properties; however, olive oil 1° and olive oil 0.4° have high rancidity and low positive sensorial attributes. Notably, OFE helps maintain bitterness close to control in Picual, Koroneiki, Empeltre, and Arbosana oils. Furthermore, amongst the 19 tested sensory descriptors, only 8 descriptors—namely, fusty/muddy sediment, winey/vinegary/acid/sour, frostbitten olives (wet wood), rancid, fruity (green), fruity (ripe), bitter, and pungent—were successfully developed to allow characterization of the sensory quality of various olive oil categories under D-F. The present research confirmed that OFE may be used to provide stable EVOOs with higher positive sensorial qualities and lower defects and could be used as a natural antioxidant and promising strategy during the D-F process with EVOOs, not only for domestic practices but also at the industrial level.
Withering and Fermentation Affect the Transformation and Accumulation of Key Metabolites in Rougui (Camellia sinensis) for the Formation of Special Taste Characteristics
Authors: Ye Jianghua , Luo Yangxin , Wang Yulin , Zhang Qi , Zhang Shuqi , Gu Junbin , Liao Yankun , Wang Tingting , Jia Xiaoli , Wang Haibin
Published: December 2024

During the production of Wuyi rock tea, withering and fermentation play a crucial role in the primary processing of the tea, greatly influencing the development of its distinct taste characteristics. In this study, Rougui (Camellia sinensis) was selected as the research object to investigate the effects of withering and fermentation on metabolites and taste characteristics in tea leaves. The findings revealed that a total of 1249 metabolites were detected in Rougui leaves at various processing stages, of which only 40 key metabolites were significantly altered. The process of withering and fermentation is crucial to increase the content of organic acids, plumerane, alkaloids, nucleotides and derivatives, amino acids and derivatives, and free fatty acids in the leaves of Rougui and to decrease the content of saccharides, phenolic acids, flavonols, flavones, and flavanols, which in turn enhances the mellowness, fresh and brisk taste, and aroma of tea and attenuates the saccharides, bitterness, and astringency. Withering and fermentation had the greatest effect on the bitterness and astringency of Rougui taste characteristics, followed by mellowness. It can be seen that withering and fermentation were extremely important for the development of Rougui’s special taste characteristics. The present study provides important support for optimizing Rougui processing and the formation of its special taste characteristics.
Detection of Pear Quality Using Hyperspectral Imaging Technology and Machine Learning Analysis
Authors: Zhang Zishen , Cheng Hong , Chen Meiyu , Zhang Lixin , Cheng Yudou , Geng Wenjuan , Guan Junfeng
Published: December 2024

The non-destructive detection of fruit quality is indispensable in the agricultural and food industries. This study aimed to explore the application of hyperspectral imaging (HSI) technology, combined with machine learning, for a quality assessment of pears, so as to provide an efficient technical method. Six varieties of pears were used for inspection, including ‘Sucui No.1’, ‘Zaojinxiang’, ‘Huangguan’, ‘Akizuki’, ‘Yali’, and ‘Hongli No.1’. Spectral data within the 398~1004 nm wavelength range were analyzed to compare the predictive performance of the Least Squares Support Vector Machine (LS-SVM) models on various quality parameters, using different preprocessing methods and the selected feature wavelengths. The results indicated that the combination of Fast Detrend-Standard Normal Variate (FD-SNV) preprocessing and Competitive Adaptive Reweighted Sampling (CARS)-selected feature wavelengths yielded the best improvement in model predictive ability for forecasting key quality parameters such as firmness, soluble solids content (SSC), pH, color, and maturity degree. They could enhance the predictive capability and reduce computational complexity. Furthermore, in order to construct a quality prediction model, integrating hyperspectral data from six pear varieties resulted in an RPD (Ratio of Performance to Deviation) exceeding 2.0 for all the quality parameters, indicating that increasing the fruit sample size and variety number further strengthened the robustness of the model. The Backpropagation Neural Network (BPNN) model could accurately distinguish six distinct pear varieties, achieving prediction accuracies of above 99% for both the calibration and test sets. In summary, the combination of HSI and machine learning models enabled an efficient, rapid, and non-destructive detection of pear quality and provided a practical value for quality control and the commercial processing of pears.
Protective Effect of Lactobacillus plantarum R2 and Lactobacillus sakei B2 on Low-Salt Sliced Sausages Stored at 5 °C
Authors: Luo Huiting , Xu Mei , Li Peijun
Published: December 2024

This study aimed to investigate the protective effects of inoculating Lactobacillus plantarum R2 and Lactobacillus sakei B2 on low-salt sliced chicken sausages during storage at 5 °C. The results demonstrated that L. plantarum R2 inhibited the growth of Pseudomonas fluorescens (p < 0.05). The results of the high-throughput sequencing indicated that the chicken sausage inoculated with L. plantarum R2 improved the microbiological quality of the sample. The levels of thiobarbituric acid reactive substances and carbonyl content of the sausages treated with L. plantarum R2 and L. sakei B2 were lower than those of the control (p < 0.05). L. plantarum R2 exhibited a higher antioxidant activity compared to that of L. sakei B2. Therefore, L. plantarum R2 was found to have the potential to improve physicochemical properties, organoleptic characteristics, and food safety of low-salt sliced cooked chicken sausages.
Study on the Extraction Technology and Antioxidant Capacity of Rhodymenia intricata Polysaccharides
Authors: Dong Shiyi , Wu Yu , Luo Yutong , Lv Wanxia , Chen Shuyi , Wang Nannan , Meng Meihan , Liao Ke , Yang Yichao
Published: December 2024

A red alga named Rhodymenia intricata was explored, and the extraction technology and antioxidant capacity of its polysaccharides were investigated. The crude polysaccharides were extracted using the ultrasound-assisted water extraction method, precipitated by alcohol, and purified using the trichloroacetic acid method. Subsequently, the scavenging rates of polysaccharides on hydroxyl, DPPH, and ABTS free radicals, were determined both prior to and following purification to evaluate their antioxidant activity. Extraction technology was optimized to improve polysaccharide yield, and the optimal parameters were as follows: particle size 100 mesh, material–liquid ratio 1:84 (g/mL), ultrasonic time 30 min, and extraction for 95 min at 80 °C. The maximized extraction rate of crude polysaccharides was 37.78 ± 0.15%. The obtained crude polysaccharides were purified with different concentrations of trichloroacetic acid, and the purification effect was evaluated according to protein removal rate and polysaccharide retention rate, which could reach 62.61 ± 1.82% and 96.10 ± 1.60%, respectively. Infrared spectrum analysis suggested that Rhodymenia intricata polysaccharide might be α-pyranose. The Congo red test illustrated that the polysaccharide contained a triple helix structure. In the antioxidant activity assessment, the scavenging rates of polysaccharide prior to purification for RIP-1 (10 mg/mL) for hydroxyl, DPPH, and ABTS free radicals were observed to achieve maximum values of 94.71 ± 0.13%, 42.80 ± 7.12%, and 76.30 ± 5.20%, respectively. In contrast, the scavenging rates of polysaccharide following purification for RIP-2 (10 mg/mL) for the same free radicals reached maximum values of 94.10 ± 0.27%, 32.37 ± 0.78%, and 98.30 ± 0.34%, respectively. Notably, these scavenging rates exhibited a dose-dependent relationship. These results demonstrated the potential of the extraction method for polysaccharides from Rhodymenia intricata, and for adding value to the by-product for its potential application as an antioxidant in food and pharmaceutical products.
Development of 3D Printable Silver Carp (Hypophthalmichthys molitrix) Surimi Gel with Dynamic High-Pressure Microfluidization-Modified Pea Protein Isolate and Microcrystalline Cellulose
Authors: Liu Xiaodan , Le Qianyu , Shi Yi , Yu Ying , Zeng Jihao , Chen Huiyun , Wu Jinhong
Published: December 2024

Sliver carp is a nutritious and abundant species in China, but its low market value stems from its thin meat, small bones and strong odor. Processing it into surimi enhances its economic value, though surimi typically has low gel strength and is prone to deterioration. Recently, three-dimensional (3D) printing has gained attention as an innovative additive manufacturing technique for personalization and process simplification requiring high-performance materials. This study intended to develop an optimized surimi formula for 3D printing with dynamic high-pressure microfluidization (DHPM)-modified pea protein isolate (PPI) and microcrystalline cellulose (MCC). Firstly, the effect of DHPM on PPI properties was evaluated, followed by the optimization of the surimi gel formula (72.093% water content, 3.203% PPI, 1.728% MCC, 1% salt, 1% collagen peptide and 20.976% sliver carp paste) and 3D printing parameters (2000 mm/min at 25 °C with a 1.5 mm nozzle). Rheological comparisons between the optimized surimi, surimi with commercial antifreeze and surimi with only PPI or MCC indicated that the optimized formulation exhibited clearer 3D printing outlines and reduced stickiness due to a higher recovery and lower loss modulus. These results demonstrated that DHPM-treated PPI and MCC enhanced the 3D printability of silver carp surimi gel, providing a new idea for a surimi product and supporting its potential applications in food 3D printing.
Analysis of the Changes in Volatile Components During the Processing of Enshi Yulu Tea
Authors: Gui Anhui , Ye Fei , Xue Jinjin , Wang Shengpeng , Liu Panpan , Wang Xueping , Teng Jing , Feng Lin , Xiang Jun , Zheng Pengcheng , Gao Shiwei
Published: December 2024

Volatile constituents are critical to the flavor of tea, but the changes in Enshi Yulu tea during the processing have not been clearly understood. Using headspace solid phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME/GC-MS) techniques, we analyze the aroma components of Enshi Yulu tea and changes in them during the processing stages. In total, 242 volatile compounds were identified. From fresh leaves to the shaping process in tea production, there are significant decreases in overall aroma substances, followed by increases after drying. Linalool is the dominant aroma component in Enshi Yulu tea, with a proportion of 12.35%, followed by compounds such as geraniol (7.41%), 2,6-dimethyl-5-heptene (6.93%), phenylmethanol (5.98%), isobutyl acetate (4.16%), hexan-1-ol (3.95%), 2-phenylacetaldehyde (3.80%), and oct-1-ene-3-ol (3.34%). The number of differential volatile components varied by production stage, with 20 up- and 139 down-regulated after steaming, 24 down-regulated after rolling, 60 up- and 51 down-regulated after shaping, and 68 up- and 13 down-regulated after drying. Most variation in expression occurred because of steaming, and the least during the rolling stage. PLS-DA analysis revealed significant differences in aroma components throughout processing and the identification of 100 compounds with higher relative contents, with five distinct change trends. Phenylmethanol, phenylacetaldehyde, (2E)-non-2-enal, oct-1-ene-3-ol, and cis-3-hexenyl hexanoate could exert a profound influence on the overall aroma quality of Enshi Yulu tea during processing. The results offer a scientific foundation and valuable insights for understanding the volatile composition of Enshi Yulu tea and its changes during the processing.
The Effects of Daily Consumption of Functionalized Yogurts with Sacha Inchi Oil and Interspecific Hybrid Palm Oil on the Lipid Profile and ApoB/ApoA1 Ratio of Healthy Adult Subjects
Authors: Villamil Ruby-Alejandra , Romero Laura-Natalia , Ruiz Juan-Pablo , Patiño Diana-Cristina , Gutiérrez Luis-Felipe , Cortés Lilia-Yadira
Published: December 2024

Sacha Inchi oil (SIO) and hybrid palm oil (HPO) are potential sources of unsaturated fatty acids to improve the lipid profile of dairy products. This study evaluated, for the first time, the effects of the daily consumption of yogurts with enhanced fatty acid profiles on plasma lipids related to cardiovascular disease (CVD) risk factors. A pilot, randomized, double-blind, parallel-controlled trial was conducted with 47 participants assigned to three groups: SIO-enriched yogurt (Group A), HPO-enriched yogurt (Group B), and plain yogurt (Group C). Fasting blood samples were collected at baseline and after 1, 2, and 3 months to measure plasma lipids (TC, LDL-C, HDL-C, and TAG), ApoA1, and ApoB. While no significant changes were observed in the overall lipid profiles, notable within-group effects were identified. The total cholesterol (TC) dropped by 2.8%, 1.3%, and 3.3%, and LDL-C by 1.6%, 2.5%, and 2% in Groups A, B, and C, respectively. Additionally, the intake of monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA), and vitamin E significantly increased in Groups A and B. These results suggest that SIO and HPO can be used as milk fat substitutes to enhance the nutritional profile of yogurts without affecting CVD biomarkers in healthy individuals.
Effect of Temperature Conditions on the Physicochemical Quality of Aged Black Garlic
Authors: Shin Jung-Hye , Kang Min-Jung , Lee Bo Hyun , Kang Dawon
Published: December 2024

This study investigates the effects of different temperature conditions on the quality of black garlic (BG) during the aging process. Two temperature protocols were employed: gradual heating and cooling (GHC), where the temperature was slowly raised from 45 °C to 77 °C and then lowered to 59 °C at a rate of 1 °C per hour, and rapid heating and cooling (RHC), where the temperature was quickly raised from 45 °C to 85 °C and then lowered to 56 °C at a rate of 1 °C every 30 min. Changes in surface color, hardness, moisture, pH, fructose, total polyphenol content (TPC), and key sulfur compounds such as alliin, S-allylcysteine (SAC), and γ-glutamyl-S-allylcysteine (γ-GSAC) were analyzed. Our findings showed that GHC led to a higher increase in TPC and fructose content by the 15th day compared to RHC. In contrast, RHC retained significantly higher SAC concentrations, approximately 1.7 times that of GHC, by the end of the aging period. Surface color changes, particularly in lightness and redness, were more pronounced under GHC, while RHC demonstrated superior moisture retention. These findings indicate that GHC is better suited for products prioritizing polyphenols and sugars, while RHC is more optimal for SAC-enriched BG. This study provides valuable insights into optimizing BG production for diverse food and medicinal applications through precise temperature modulation.
Regular Family Meals Associated with Nutritional Status, Food Consumption, and Sedentary and Eating Behaviors of Brazilian Schoolchildren and Their Caregivers
Authors: Oliveira Giovanna Angela Leonel , Buccini Gabriela , Gonçalves Vivian S. S. , Gubert Muriel Bauermann , Toral Natacha
Published: December 2024

The influence of family meals on nutrition and health for families has been understudied, especially in low- and middle-income countries. We aimed to analyze associations between regular family meals and body mass index (BMI), food consumption, eating, and sedentary behaviors among Brazilian schoolchildren and their caregivers. A cross-sectional study was conducted with 1887 Brazilian schoolchildren aged 6–11 years and their caregivers. Caregivers provided sociodemographic data, their child’s weight and height, and the frequency of family meals. Schoolchildren provided data on gender and silhouette scale. Both provided their food consumption, eating, and sedentary behavior data. Associations between regular family meals and BMI, ultra-processed food consumption, and dietary diversity were assessed through multivariate logistic regression models. Correlations of regular family meals with eating and sedentary behaviors were assessed using Pearson chi-square. Regular family meals were frequent (86.6%), and they were associated with less unhealthy BMI in caregivers (AOR: 0.74; 95%CI: 0.5–0.9), as well as higher dietary diversity in caregivers (AOR: 1.66; 95%CI: 1.0–2.7) and in schoolchildren (AOR: 1.78; 95%CI: 1.4–2.3). The coexistence of high dietary diversity and both low ultra-processed food consumption (AOR: 1.45; 95%CI: 1.0–2.1) and healthy BMI (AOR: 1.41; 95%CI: 1.0–1.9) in children was associated with regular family meals. Regular family meals were correlated with healthy eating behaviors in child-caregiver dyads (p < 0.002) and with sedentary behavior in caregivers (p = 0.019). Our findings underscore regular family meals as a protective factor against malnutrition among Brazilian families.
Effects of a Diet of Allium Extract on Growth, Biochemistry, Metabolism, and Gut Microbiota of Rabbits (Oryctolagus cuniculus)
Authors: Cascajosa-Lira Antonio , Pichardo Silvia , Baños Alberto , Aguinaga-Casañas María Arántzazu , Ricci Andrea , Frabetti Andrea , Barausse Andrea , Jos Angeles , Cameán Ana M.
Published: December 2024

The rabbit farming industry is growing due to the rising demand for healthy, sustainable meat. Rabbit meat’s nutritional benefits and low environmental impact appeal to health-conscious consumers. To enhance economic sustainability, efforts focus on reducing disease susceptibility and antibiotic use through improved biosecurity and natural additives, such as organosulphur compounds from Allium plants, which have shown promise in studies for boosting productivity and health. This study aimed to investigate the effects of PTSO supplementation on farm rabbits. Over and after a 76-day period, various parameters were assessed to measure the impacts on rabbit growth, health, biochemical parameters, muscle metabolism, and intestinal microbiota. The rabbit groups received either a control diet or a diet supplemented with Allium extract. The results showed significant improvements in growth performance for rabbits fed with Allium extract, including higher final weights, increased average daily gain (ADG), and lower mortality rates. A biochemical analysis revealed normal values for the parameters measured in the treated group. A muscle analysis evidenced the presence of a few metabolites of PTSO. A gut microbiota analysis indicated distinct differences between the control and treated groups, increasing the presence of some strains that can influence positively the growth of rabbits. This study highlights the potential benefits of PTSO supplementation for improving growth performance, health parameters, and gut microbiota composition in farm rabbits, suggesting its efficacy as a dietary additive.
Characteristics of Meat from Farmed Sika Deer (Cervus nippon) and the Effects of Age and Sex on Meat Quality
Authors: Peng Zhangrong , Zhao Hui , Luo Jing , Sun Haoran , Jiang Qingkui , Zhang Tietao
Published: December 2024

This study assessed the meat quality of Sika deer (Cervus nippon) from various age and sex groups using the longissimus dorsi (LD) muscle. Samples from different age groups (2, 3, and 4 years old) were analyzed for various parameters. The results show that, with increasing age, there is a decrease in moisture and drip loss (p < 0.05), alongside increases in ash, protein, fat, and cooking loss (p < 0.05). Female deer showed an increase in tenderness (p < 0.05), whereas males generally had a higher amino acid content (p < 0.05). Interestingly, 2-year-old female Sika deer had more saturated fatty acids (p < 0.05), while 3- and 4-year-old females had more unsaturated fatty acids compared to age-matched males (p < 0.05). Notably, 3-year-olds had higher levels of monounsaturated and polyunsaturated fatty acids in both the male and female groups (p < 0.05). Overall, this study provides the first comprehensive evidence that Sika deer meat is a nutritious source of lean protein. Notably, meat from 3-year-old Sika deer, regardless of sex, contains higher nutrient levels and is more tender compared to meat from deer of other ages. Additionally, meat from females tends to be more tender than that from males.
Evaluation of Microwave Heating Uniformity for Ready-to-Eat Rice in Metalized Packaging Structure
Authors: Liu Chai , Tian Bo , Liu Huiran , Shen Liuyang , Zhu Yong , Liu Chenghai , Zheng Xianzhe , Deng Xiting , Zhao Yuxin
Published: December 2024

Microwave energy utilization undergoes two stages via absorption and conversion inside ready-to-eat rice (RER) under microwave reheating. The reasonable utilization of microwave energy inside the processed material may enhance the uniformity of the temperature distribution. To analyze the uniformity changes inside RER, the effects of microwave reflection, refraction, and absorption by a metal aluminum film were studied through the thermodynamic properties. A simulation model was developed using the co-simulation method of COMSOL Multiphysics with MATLAB programming to analyze the mechanism of material properties and electromagnetic distribution on temperature distribution uniformity, as well as the formation mechanism of the temperature distribution uniformity of microwave-reheated RER. Based on models of the designed package boxes covering the metal film, the optimal structure was developed to include a metal aluminum film with a width of 5 mm and a thickness of 0.30 mm, which was sprayed on the edges and corners of a rectangular packaging box. The packaging boxes covering the metal films may reduce the average temperature of the upper and lower layers in RER by 8.03 °C and 7.42 °C, respectively, while the temperature distribution uniformity increased by 35.71% and 72.22%. The introduction of a metalized package significantly enhances the temperature uniformity inside RER under microwave reheating.
Preparation and Performance Evaluation of CO2 Foam Gel Fracturing Fluid
Authors: Gao Yan , Yang Jiahui , Li Zefeng , Ma Zhenfeng , Xu Xinjie , Liu Ruiqiong , Li Xin , Zhang Lixiao , Zhao Mingwei
Published: December 2024

The utilization of CO2 foam gel fracturing fluid offers several significant advantages, including minimal reservoir damage, reduced water consumption during application, enhanced cleaning efficiency, and additional beneficial properties. However, several current CO2 foam gel fracturing fluid systems face challenges, such as complex preparation processes and insufficient viscosity, which limit their proppant transport capacity. To address these issues, this work develops a novel CO2 foam gel fracturing fluid system characterized by simple preparation and robust foam stability. This system was optimized by incorporating a thickening agent CZJ-1 in conjunction with a foaming agent YFP-1. The results of static sand-carrying experiments indicate that under varying temperatures and sand–fluid ratio conditions, the proppant settling velocity is significantly low. Furthermore, the static sand-carrying capacity of the CO2 foam gel fracturing fluid exceeds that of the base fluid. The stable and dense foam gel effectively encapsulates the proppant, thereby improving sand-carrying capacity. In high-temperature shear tests, conducted at a shear rate of 170 s−1 and a temperature of 110 °C for 90 min, the apparent viscosity of the CO2 foam gel fracturing fluid remained above 20 mPa·s after shear, demonstrating excellent high-temperature shear resistance. This work introduces a novel CO2 foam gel fracturing fluid system that is specifically tailored for low-permeability reservoir fracturing and extraction. The system shows significant promise for the efficient development of low-pressure, low-permeability, and water-sensitive reservoirs, as well as for the effective utilization and sequestration of CO2.
Activation Energy of SDS–Protein Complexes in Capillary Electrophoresis with Tetrahydroxyborate Cross-Linked Agarose Gels
Authors: Sárközy Dániel , Guttman András
Published: December 2024

Hydrogels like agarose have long been used as sieving media for the electrophoresis-based analysis of biopolymers. During gelation, the individual agarose strands tend to form hydrogen-bond mediated double-helical structures, allowing thermal reversibility and adjustable pore sizes for molecular sieving applications. The addition of tetrahydroxyborate to the agarose matrix results in transitional chemical cross-linking, offering an additional pore size adjusting option. Separation of SDS-proteins during gel electrophoresis is an activated process defined by the interplay between viscosity, gelation/cross-link formation/distortion, and sample conformation. In this paper, the subunits of a therapeutic monoclonal antibody were separated by capillary SDS agarose gel electrophoresis at different temperatures. The viscosity of the separation matrix was also measured at all temperatures. In both instances, Arrhenius plots were used to obtain the activation energy values. It was counterintuitively found that larger SDS–protein complexes required lower activation energies while their low-molecular-weight counterparts needed higher activation energy for their electromigration through the sieving matrix. As a first approximation, we considered this phenomenon the result of the electric force-driven distortion of the millisecond range lifetime reticulations by the larger and consequently more heavily charged electromigrating molecules. In the meantime, the sieving properties of the gel were still maintained, i.e., they allowed for the size-based separation of the sample components, proving the existence of the reticulations. Information about the activation energy sheds light on the possible deformation of the sieving matrix and the solute molecules. In addition, the activation energy requirement study helped in optimizing the separation temperature, e.g., with our sample mixture, the highest resolution was obtained for the high-molecular-weight fragments, i.e., between the non-glycosylated heavy chain and heavy-chain subunits at 25 °C (lower Ea requirement), while 55 °C was optimal for the lower-molecular-weight light chain and non-glycosylated heavy chain pair (lower Ea requirement). Future research directions and possible applications are also proposed.
Detecting N-Phenyl-2-Naphthylamine, L-Arabinose, D-Mannose, L-Phenylalanine, L-Methionine, and D-Trehalose via Photocurrent Measurement
Authors: Li Feng , Yang Ruoxi , Xu Jian , Xu Guohai , Wu Ye
Published: December 2024

The concentration of small molecules reflects the normality of physiological processes in the human body, making the development of simple and efficient detection equipment essential. In this work, inspired by a facile strategy in point-of-care detection, two devices were fabricated to detect small molecules via photocurrent measurement. A linear response of the photocurrent against the concentration of the small molecules was found. In the first device, metal ions were introduced into gel substrates made by xanthan gum to enhance photocurrent response. N-phenyl-2-naphthylamine was measured when iron or manganese ions were used. L-Phenylalanine was measured when the gel was modified by samarium, iron, cerium, or ytterbium ions. L-(+)-Arabinose was detected via the gels modified by iron or holmium ions. D-(+)-Mannose was detected when the gel was modified by ytterbium, manganese, chromium, or sodium ions. L-Methionine was detected in the gels modified by samarium, zinc, or chromium ions. The second device was based on a paper sheet. A sugar-like molecule was first synthesized, which was then used to modify the paper. The detection was possible since the photocurrent showed a linear trend against the concentration of D-Trehalose. A linear fit was conducted to derive the sensitivity, whose value was found to be 5542.4. This work offers a novel, simple, and environmentally sustainable platform that is potentially useful in remote areas lacking medical devices.
Ice-Jam Flooding of the Peace–Athabasca Delta, Canada: Insights from Recent Notable Spring Breakup Events and Implications for Strategic Flow Releases from Upstream Dams
Authors: Beltaos Spyros
Published: December 2024

Ice jamming is the primary mechanism that can generate overland flooding and recharge the isolated basins of the Peace–Athabasca Delta (PAD), a valuable ecosystem of international importance and the ancient homeland of the Indigenous Peoples of the region. Focusing on the regulated Peace River and the Peace Sector of the delta, which has been experiencing a drying trend in between rare ice-jam floods over the last ~50 years, this study describes recent notable breakup events, associated observational data, and numerical applications to determine river discharge during the breakup events. Synthesis and interpretation of this material provide a new physical understanding that can inform the ongoing development of a protocol for strategic flow releases toward enhancing basin recharge in years when major ice jams are likely to form near the PAD. Additionally, several recommendations are made for future monitoring activities and improvements in proposed antecedent criteria for early identification of “promising” breakup events.