bluuebunny/crossref_metadata_2025 huggingface dataset:
Cloud-Based CAD Parametrization for Design Space Exploration and Design Optimization in Numerical Simulations
Authors: Guerrero Joel , Mantelli Luca , Naqvi Sahrish B.
Published: March 2020

In this manuscript, an automated framework dedicated to design space exploration and design optimization studies is presented. The framework integrates a set of numerical simulation, computer-aided design, numerical optimization, and data analytics tools using scripting capabilities. The tools used are open-source and freeware, and can be deployed on any platform. The main feature of the proposed methodology is the use of a cloud-based parametrical computer-aided design application, which allows the user to change any parametric variable defined in the solid model. We demonstrate the capabilities and flexibility of the framework using computational fluid dynamics applications; however, the same workflow can be used with any numerical simulation tool (e.g., a structural solver or a spread-sheet) that is able to interact via a command-line interface or using scripting languages. We conduct design space exploration and design optimization studies using quantitative and qualitative metrics, and, to reduce the high computing times and computational resources intrinsic to these kinds of studies, concurrent simulations and surrogate-based optimization are used.
Effects of Nitrogen Application in the Wheat Booting Stage on Glutenin Polymerization and Structural–Thermal Properties of Gluten with Variations in HMW-GS at the Glu-D1 Locus
Authors: Song Lijun , Li Liqun , Zhao Liye , Liu Zhenzhen , Li Xuejun
Published: March 2020

Wheat gluten properties can be improved by the application of nitrogen. This study investigates the effects of nitrogen application in the booting stage on glutenin polymerization during grain-filling and structural–thermal properties of gluten based on the high-molecular-weight glutenin subunits (HMW-GSs) using near-isogenic lines (Glu-1Da and Glu-1Dd). The nitrogen rate experiment included rates of 0, 60, 90, and 120 kg N ha−1 applied with three replicates. Nitrogen significantly improved the grain quality traits (wet gluten contents, Zeleny sedimentation values, and maximum resistance) and dough strength (dough development time, dough stability time, and protein weakening), especially in wheat with the Glu-1Da allele. Nitrogen increased the protein composition contents, proportions of glutenins and HMW-GSs, and disulfide bond concentration in the flours of Glu-1Da and Glu-1Dd, and accelerated the polymerization of glutenins (appearing as glutenin macropolymer) during grain-filling, where nitrogen enhanced the accumulation and polymerization of glutenins more for line containing Glu-1Da than Glu-1Dd. The β-sheets, α-helix/β-sheet ratio, microstructures, and thermal stability were also improved to a greater degree by nitrogen for gluten with Glu-1Da compared to Glu-1Dd. Nitrogen treatment was highly effective at improving the gluten structural‒thermal properties of wheat in the booting stage, especially with inferior glutenin subunits.
Post-Analytical Tools for the Triage of Newborn Screening Results in Follow-up Can Reduce Confirmatory Testing and Guide Performance Improvement
Authors: Hall Patricia L. , Wittenauer Angela , Hagar Arthur
Published: March 2020

Georgia uses post-analytical tools through Collaborative Laboratory Integrated Reports (CLIR) to triage abnormal newborn screening (NBS) results for follow-up. Condition specific tools are used to assign each case a risk level, which is used to guide follow-up recommendations. Follow-up recommendations include assessment by the child’s primary care provider as well as testing, either a repeat NBS or confirmatory testing. Triaging abnormal cases using these tools has been advantageous in managing the workflow for the follow-up team, as well as prioritizing cases that appropriately require more attention and resources. The initial goal in utilizing these tools was to reduce the amount of confirmatory testing, particularly for disorders where there are many false positives. We assessed the performance of these tools retrospectively for three of the most commonly detected conditions by tandem mass spectrometry in Georgia: phenylketonuria, medium chain acyl-CoA dehydrogenase deficiency and very long chain dehydrogenase deficiency. The post-analytical tools appropriately assigned all true positive cases to the higher levels of follow-up testing and reduced the level of intervention for a significant number of cases as well. Based on the experience gained from our utilization of the tools in the follow-up program, we are well situated to move forward with using the tools in a more prospective manner, and reduce the number of cases that will be reported, rather than just assigning resources appropriately at follow-up. Post-analytical tools are an improvement over trying to capture the variation in the newborn population using multiple cutoffs. It also easily identifies significant abnormalities that are unrelated to inherited disease, such as large amino acid elevations due to total parenteral nutrition.
Functional Characterization of Sex Pheromone Receptors in the Fall Armyworm (Spodoptera frugiperda)
Authors: Guo Jin-Meng , Liu Xiao-Long , Liu Si-Ruo , Wei Zhi-Qiang , Han Wei-Kang , Guo Youzhong , Dong Shuang-Lin
Published: March 2020

Pheromone receptors (PRs) found in the antennae of male moths play a vital role in the recognition of sex pheromones released by females. The fall armyworm (FAW), Spodoptera frugiperda, is a notorious invasive pest, but its PRs have not been reported. In this report, six candidate PRs (SfruOR6, 11, 13, 16, 56 and 62) suggested by phylogenetic analysis were cloned, and their tissue–sex expression profiles were determined by quantitative real-time PCR (qPCR). All six genes except for SfruOR6 were highly and specifically expressed in the antennae, with SfruOR6, 13 and 62 being male-specific, while the other three (SfruOR11, 16 and 56) were male biased, suggesting their roles in sex pheromone perception. A functional analysis by the Xenopus oocyte system further demonstrated that SfruOR13 was highly sensitive to the major sex pheromone component Z9-14:OAc and the pheromone analog Z9,E12-14:OAc, but less sensitive to the minor pheromone component Z9-12:OAc; SfruOR16 responded weakly to pheromone component Z9-14:OAc, but strongly to pheromone analog Z9-14:OH; the other four candidate PRs did not respond to any of the four pheromone components and four pheromone analogs. This study contributes to clarifying the pheromone perception in the FAW, and provides potential gene targets for developing OR-based pest control techniques.
Timing of Heparin Administration Modulates Arterial Occlusive Thrombotic Response in Rats
Authors: Matrai Amanda B. , Kastetter Bryn , Cooley Brian C.
Published: March 2020

Background: The timing for initiation of effective antithrombotic therapy relative to the onset of arterial thrombosis may influence outcomes. This report investigates the hypothesis that early administration of heparin anticoagulation relative to the onset of thrombotic occlusion will effect a reduction in occlusion. Methods: A standard rat model of experimental thrombosis induction was used, injuring the carotid artery exposure with FeCl3-saturated filter paper, followed by flow monitoring for onset of occlusion and subsequent embolization events. Intravenous heparin administration (200 units/mL) was timed relative to the initiation of injury or onset of near occlusion, compared with controls (no heparin administration). Results: No occlusion was found for delivery of heparin 5 min prior to thrombus induction, whereas all vessels occluded without heparin. Unstable (embolic) thrombi were seen with heparin given at or shortly after initial occlusion. Only 9% (1/11) of the vessels had permanent occlusion when heparin was given at the time of thrombotic onset (p < 0.0001 vs. unheparinized), while 50% occluded when heparin was delayed by 5 min (p > 0.05). Conclusions: These findings provide evidence that antithrombotic therapy may need to be administered prior to the onset of anticipated loss of patency, with less effectiveness when given after occlusion has occurred.
Comparative In Vitro Study of Various α2-Adrenoreceptor Agonist Drugs for Ticagrelor Reversal
Authors: Porta Bonete Guillaume , Godier Anne , Gaussem Pascale , Belleville-Rolland Tiphaine , Leuci Alexandre , Poirault-Chassac Sonia , Bachelot-Loza Christilla , Martin Anne-Céline
Published: March 2020

Ticagrelor, an antiplatelet adenosine diphosphate (ADP)-P2Y12 receptor antagonist, increases the risk of bleeding. Its management is challenging because platelet transfusion is ineffective and no specific antidote is currently available. Epinephrine, a vasopressor catecholamine prescribed during shock, restores platelet functions inhibited by ticagrelor through stimulation of α2A-adrenoreceptors. It subsequently inhibits cyclic adenosine monophosphate (cAMP) pathway and PI3K signaling. However, since epinephrine may expose a patient to deleterious hemodynamic effects, we hypothesized that other α2-adrenoreceptor agonist drugs used in clinical practice with fewer side effects could reverse the antiplatelet effects of ticagrelor. We compared in vitro the efficacy of clonidine, dexmedetomidine, brimonidine, and norepinephrine with epinephrine to restore ADP- and PAR-1-AP-induced washed platelet aggregation inhibited by ticagrelor, as well as resulting platelet cAMP levels. In ticagrelor-free samples, none of the α2-adrenoreceptor agonists induced aggregation by itself but all of them potentiated ADP-induced aggregation. Compared with epinephrine, norepinephrine, and brimonidine partially restored ADP- and fully restored PAR-1-AP-induced aggregation inhibited by ticagrelor while clonidine and dexmedetomidine were ineffective. Indeed, this lack of effect resulted from a lower decrease in cAMP concentration elicited by these partial α2-adrenoreceptor agonists, clonidine, and dexmedetomidine, compared with full α2-agonists. Our results support the development of specific full and systemic α2-adrenoreceptor agonists for ticagrelor reversal.
Health in Preconception, Pregnancy and Postpartum Global Alliance: International Network Pregnancy Priorities for the Prevention of Maternal Obesity and Related Pregnancy and Long-Term Complications
Authors: Hill Briony , Skouteris Helen , Boyle Jacqueline A. , Bailey Cate , Walker Ruth , Thangaratinam Shakila , Sundseth Hildrun , Stephenson Judith , Steegers Eric , Redman Leanne M. , Montanaro Cynthia , Lim Siew , Jorgensen Laura , Jack Brian , Borges Ana Luiza Vilela , Bergmeier Heidi J. , Baxter Jo-Anna B. , Harrison Cheryce L. , Teede Helena J.
Published: March 2020

In this article, we describe the process of establishing agreed international pregnancy research priorities to address the global issues of unhealthy lifestyles and rising maternal obesity. We focus specifically on the prevention of maternal obesity to improve related clinical pregnancy and long-term complications. A team of multidisciplinary, international experts in preconception and pregnancy health, including consumers, were invited to form the Health in Preconception, Pregnancy and Postpartum (HiPPP) Global Alliance. As an initial activity, a priority setting process was completed to generate pregnancy research priorities in this field. Research, practice and policy gaps were identified and enhanced through expert and consumer consultation, followed by a modified Delphi process and Nominal Group Technique, including an international workshop. Research priorities identified included optimising: (1) healthy diet and nutrition; (2) gestational weight management; (3) screening for and managing pregnancy complications and pre-existing conditions; (4) physical activity; (5) mental health; and (6) postpartum (including intrapartum) care. Given extensive past research in many of these areas, research priorities here recognised the need to advance pregnancy research towards pragmatic implementation research. This work has set the agenda for large-scale, collaborative, multidisciplinary, implementation research to address the major public health and clinical issue of maternal obesity prevention.
Effect of a Preoperative Proton Pump Inhibitor and Gastroesophageal Reflux Disease on Postoperative Nausea and Vomiting
Authors: Kwon Young Suk , Choi Jun Woo , Lee Ho Seok , Kim Jong Ho , Kim Youngmi , Lee Jae Jun
Published: March 2020

Postoperative nausea and vomiting (PONV) are common complications after anesthesia, but no study has considered the effects of a proton pump inhibitor (PPI) and gastroesophageal reflux disease (GERD) on PONV at the same time. Thus, we investigated the effects of a PPI and GERD on PONV. Patients aged ≥18 years who underwent general anesthesia between 2010 and 2019 were enrolled. In total, 202,439 patients were included and 21,361 In a multivariate analysis, the OR for PONV was higher in subjects with GERD (OR, 1.157; 95% CI, 1.032–1.298; p = 0.012). The OR was lower for subjects with taking a PPI (OR, 0.890; 95% CI, 0.832–0.953; p < 0.0001). In patients without GERD, the incidence of PONV was lower when lansoprazole (OR, 0.801; 95% CI, 0.718–0.894; p < 0.0001), pantoprazole (OR, 0.856; 95% CI, 0.748–0.980; p = 0.025) and ilaprazole (OR, 0.391; 95% CI, 0.158–0.966; p = 0.042) were taken. However, in GERD patients, all PPIs did not show reducing the incidence of PONV. Taken together, the results show that a lansoprazole, pantoprazole, and ilaprazole reduced PONV in patients without GERD, and PPI could not reduce PONV in patients with GERD.
Network Mapping of Connectivity Alterations in Disorder of Consciousness: Towards Targeted Neuromodulation
Authors: Mencarelli Lucia , Biagi Maria Chiara , Salvador Ricardo , Romanella Sara , Ruffini Giulio , Rossi Simone , Santarnecchi Emiliano
Published: March 2020

Disorder of consciousness (DoC) refers to a group of clinical conditions that may emerge after brain injury, characterized by a varying decrease in the level of consciousness that can last from days to years. An understanding of its neural correlates is crucial for the conceptualization and application of effective therapeutic interventions. Here we propose a quantitative meta-analysis of the neural substrate of DoC emerging from functional magnetic resonance (fMRI) and positron emission tomography (PET) studies. We also map the relevant networks of resulting areas to highlight similarities with Resting State Networks (RSNs) and hypothesize potential therapeutic solutions leveraging network-targeted noninvasive brain stimulation. Available literature was reviewed and analyzed through the activation likelihood estimate (ALE) statistical framework to describe resting-state or task-dependent brain activation patterns in DoC patients. Results show that task-related activity is limited to temporal regions resembling the auditory cortex, whereas resting-state fMRI data reveal a diffuse decreased activation affecting two subgroups of cortical (angular gyrus, middle frontal gyrus) and subcortical (thalamus, cingulate cortex, caudate nucleus) regions. Clustering of their cortical functional connectivity projections identify two main altered functional networks, related to decreased activity of (i) the default mode and frontoparietal networks, as well as (ii) the anterior salience and visual/auditory networks. Based on the strength and topography of their connectivity profile, biophysical modeling of potential brain stimulation solutions suggests the first network as the most feasible target for tES, tDCS neuromodulation in DoC patients.
The Influence and Action Mechanization of Mineral Mixed Material on High Fluidity Potassium Magnesium Phosphate Cement (MKPC)
Authors: Wu Qing , Zou Yan , Gu Jianhua , Xu Jun , Ji Rongjian , Wang Gang
Published: March 2020

Potassium magnesium phosphate cement (MKPC) is a type of chemically bonded ceramic material that has higher performance compared to traditional Portland cement. To develop the spraying and crack pouring process of MKPC, the mechanical properties, volume deformation, hydration temperature, and water stability of the high-fluidity MKPC with different mineral mixed materials and their influence laws were studied. The effects of phase composition and micromorphology of hydration products on the properties of MKPC and its mechanism were analyzed using X-ray diffraction (XRD), thermogravimetry/differential thermal analysis (TG/DTA), and scanning electron microscopy (SEM). The results show that fly ash and metakaolin will not reduce the fluidity of MKPC paste because of their material properties, and silica fume will reduce the fluidity of MKPC paste because of its large specific surface area and high water absorption. Metakaolin can react with phosphate to form aluminum phosphate gel and fill the pores between the crystals because it has a higher activity, which can significantly improve its compressive strength. However, during the later stage of hydration, there will be slight expansion, which would reduce its bonding flexural strength. The MKPC-hardened paste mixed with silicon ash has optimal stability: therefore, it has the highest bonding flexural strength. Microcosmic analysis shows that mineral mixed material plays a physical filling role and participates in the hydration reaction as an active ingredient to improve the early hydration degree, which can change the crystal size and micromorphology of MKPC-hardened paste and make the structure more compact.
Reliability Study of Electronic Components on Board-Level Packages Encapsulated by Thermoset Injection Molding
Authors: Kulkarni Romit , Soltani Mahdi , Wappler Peter , Guenther Thomas , Fritz Karl-Peter , Groezinger Tobias , Zimmermann André
Published: March 2020

A drastically growing requirement of electronic packages with an increasing level of complexity poses newer challenges for the competitive manufacturing industry. Coupled with harsher operating conditions, these challenges affirm the need for encapsulated board-level (2nd level) packages. To reduce thermo-mechanical loads induced on the electronic components during operating cycles, a conformal type of encapsulation is gaining preference over conventional glob-tops or resin casting types. The availability of technology, the ease of automation, and the uncomplicated storage of raw material intensifies the implementation of thermoset injection molding for the encapsulation process of board-level packages. Reliability case studies of such encapsulated electronic components as a part of board-level packages become, thereupon, necessary. This paper presents the reliability study of exemplary electronic components, surface-mounted on printed circuit boards (PCBs), encapsulated by the means of thermoset injection molding, and subjected to cyclic thermal loading. The characteristic lifetime of the electronic components is statistically calculated after assessing the probability plots and presented consequently. A few points of conclusion are summarized, and the future scope is discussed at the end.
Morphological Characterization and Genetic Diversity of Rice Blast Fungus, Pyricularia oryzae, from Thailand Using ISSR and SRAP Markers
Authors: Longya Apinya , Talumphai Sucheela , Jantasuriyarat Chatchawan
Published: March 2020

Rice blast disease is caused by the ascomycete fungus Pyricularia oryzae and is one of the most destructive rice diseases in the world. The objectives of this study were investigating various fungal morphological characteristics and performing a phylogenetic analysis. Inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers were used to examine the genetic variation of 59 rice blast fungus strains, including 57 strains collected from different fields in Thailand and two reference strains, 70-15 and Guy11. All isolates used in this study were determined to be P. oryzae by internal transcribed spacer (ITS) sequence confirmation. A total of 14 ISSR primers and 17 pairs of SRAP primers, which produced clear and polymorphic bands, were selected for assessing genetic diversity. A total of 123 polymorphic bands were generated. The similarity index value for the strains ranged from 0.25 to 0.95. The results showed that the blast fungus population in Thailand has both morphological and genetic variations. A high level of genetic variation, or genome adaptation, is one of the fungal mechanisms that could overcome host resistance to avoid host recognition. Results from this research study could bring substantial benefits and ultimately help to understand the blast fungal pathogen genome and the population structure in Thai blast fungus.
Dysmorphology in the Era of Genomic Diagnosis
Authors: Hurst Anna C. E. , Robin Nathaniel H.
Published: March 2020

Genetic and genomic testing technologies have expanded beyond levels of diagnostic capability that were unimaginable even a few years ago. While this has significantly benefited clinicians in their care of patients and families, it has also altered how geneticists evaluate patients. One immediate example is the role of the dysmorphologic physical exam in the patient evaluation. While some have suggested that it is no longer necessary, we argue that the dysmorphologic physical exam is still essential, albeit in a different role.
Bank Competition and Credit Risk in Euro Area Banking: Fragmentation and Convergence Dynamics
Authors: Karadima Maria , Louri Helen
Published: March 2020

Consolidation in euro area banking has been the major trend post-crisis. Has it been accompanied by more or less competition? Has it led to more or less credit risk? In all or some countries? In this study, we examine the evolution of competition (through market power and concentration) and credit risk (through non-performing loans) in 2005–2017 across all euro area countries (EA-19), as well as core (EA-Co) and periphery (EA-Pe) countries separately. Using Theil inequality and convergence analysis, our results support the continued existence of fragmentation as well as of divergence within and/or between core and periphery with respect to competition and credit risk, especially post-crisis, in spite of some partial reintegration trends. Policy measures supporting faster convergence of our variables would be helpful in establishing a real banking union.
Nano-Structured Dilute Magnetic Semiconductors for Efficient Spintronics at Room Temperature
Authors: Gupta Akanksha , Zhang Rui , Kumar Pramod , Kumar Vinod , Kumar Anup
Published: March 2020

In recent years, many efforts have been made to develop advanced metal oxide semiconductor nanomaterials with exotic magnetic properties for modern applications w.r.t traditional analogues. Dilute magnetic semiconductor oxides (DMSOs) are promising candidates for superior control over the charge and spin degrees of freedom. DMSOs are transparent, wide band gap materials with induced ferromagnetism in doping, with a minor percentage of magnetic 3d cation to create a long-range antiferromagnetic order. Although significant efforts have been carried out to achieve DMSO with ferromagnetic properties above room temperature, it is a great challenge that still exists. However, TiO2, SnO2, ZnO and In2O3 with wide band gaps of 3.2, 3.6, 3.2 and 2.92 eV, respectively, can host a broad range of dopants to generate various compositions. Interestingly, a reduction in the size of these binary oxides can induce ferromagnetism, even at room temperature, due to the grain boundary, presence of defects and oxygen vacancies. The present review provides a panorama of the structural analysis and magnetic properties of DMSOs based on binary metal oxides nanomaterials with various ferromagnetic or paramagnetic dopants, e.g., Co, V, Fe and Ni, which exhibit enhanced ferromagnetic behaviors at room temperature.
Maclaurin Coefficient Estimates of Bi-Univalent Functions Connected with the q-Derivative
Authors: El-Deeb Sheza M. , Bulboacă Teodor , El-Matary Bassant M.
Published: March 2020

In this paper we introduce a new subclass of the bi-univalent functions defined in the open unit disc and connected with a q-analogue derivative. We find estimates for the first two Taylor-Maclaurin coefficients a 2 and a 3 for functions in this subclass, and we obtain an estimation for the Fekete-Szegő problem for this function class.
Global Mittag–Leffler Stability and Stabilization Analysis of Fractional-Order Quaternion-Valued Memristive Neural Networks
Authors: Rajchakit Grienggrai , Chanthorn Pharunyou , Kaewmesri Pramet , Sriraman Ramalingam , Lim Chee Peng
Published: March 2020

This paper studies the global Mittag–Leffler stability and stabilization analysis of fractional-order quaternion-valued memristive neural networks (FOQVMNNs). The state feedback stabilizing control law is designed in order to stabilize the considered problem. Based on the non-commutativity of quaternion multiplication, the original fractional-order quaternion-valued systems is divided into four fractional-order real-valued systems. By using the method of Lyapunov fractional-order derivative, fractional-order differential inclusions, set-valued maps, several global Mittag–Leffler stability and stabilization conditions of considered FOQVMNNs are established. Two numerical examples are provided to illustrate the usefulness of our analytical results.
Derived Hyperstructures from Hyperconics
Authors: Vahedi Vahid , Jafarpour Morteza , Hoskova-Mayerova Sarka , Aghabozorgi Hossein , Leoreanu-Fotea Violeta , Bekesiene Svajone
Published: March 2020

In this paper, we introduce generalized quadratic forms and hyperconics over quotient hyperfields as a generalization of the notion of conics on fields. Conic curves utilized in cryptosystems; in fact the public key cryptosystem is based on the digital signature schemes (DLP) in conic curve groups. We associate some hyperoperations to hyperconics and investigate their properties. At the end, a collection of canonical hypergroups connected to hyperconics is proposed.
Stability of Replicator Dynamics with Bounded Continuously Distributed Time Delay
Authors: Zhong Chongyi , Yang Hui , Liu Zixin , Wu Juanyong
Published: March 2020

In this paper, we consider evolutionary games and construct a model of replicator dynamics with bounded continuously distributed time delay. In many circumstances, players interact simultaneously while impacts of their choices take place after some time, which implies a time delay exists. We consider the time delay as bounded continuously distributed other than some given constant. Then, we investigate the stability of the evolutionarily stable strategy in the replicator dynamics with bounded continuously distributed time delay in two-player game contexts. Some stability conditions of the unique interior Nash equilibrium are obtained. Finally, the simple but important Hawk–Dove game is used to verify our results.
Impact of Micro-Scale Stochastic Zonal Flows on the Macro-Scale Visco-Resistive Magnetohydrodynamic Modes
Authors: Moradi Sara , Thyagaraja Anantanarayanan
Published: March 2020

A model is developed to simulate micro-scale turbulence driven Zonal Flows (ZFs), and their impact on the Magnetohydrodynamic (MHD) tearing and kink modes is examined. The model is based on a stochastic representation of the micro-scale ZFs with a given Alfvén Mach number, MS. Two approaches were explored: (i) passive stochastic model where the ZFs amplitudes are independent of the MHD mode amplitude, and (ii) the semi-stochastic model where the amplitudes of the ZFs have a dependence on the amplitude of the MHD mode itself. The results show that the stochastic ZFs can significantly stabilise the (2,1) and (1,1) MHD modes even at very low kinematic viscosity, where the mode is linearly unstable. Our results therefore indicate a possible mechanism for stabilisation of the MHD modes via small-scale perturbations in poloidal flow, simulating the turbulence driven ZFs.
Chaos Synchronization for Hyperchaotic Lorenz-Type System via Fuzzy-Based Sliding-Mode Observer
Authors: Plata Corina , Prieto Pablo J. , Ramirez-Villalobos Ramon , Coria Luis N.
Published: March 2020

Hyperchaotic systems have applications in multiple areas of science and engineering. The study and development of these type of systems helps to solve diverse problems related to encryption and decryption of information. In order to solve the chaos synchronization problem for a hyperchaotic Lorenz-type system, we propose an observer based synchronization under a master-slave configuration. The proposed methodology consists of designing a sliding-mode observer (SMO) for the hyperchaotic system. In contrast, this type of methodology exhibits high-frequency oscillations, commonly known as chattering. To solve this problem, a fuzzy-based SMO system was designed. Numerical simulations illustrate the effectiveness of the synchronization between the hyperchaotic system obtained and the proposed observer.
Terpenoids from the Deep-Sea-Derived Fungus Penicillium thomii YPGA3 and Their Bioactivities
Authors: Cheng Zhongbin , Liu Wan , Fan Runzhu , Han Shouye , Li Yuanli , Cui Xiaoyun , Zhang Jia , Wu Yinan , Lv Xin , Zhang Yun , Luo Zhuhua , Alias Siti Aisyah , Xu Wei , Li Qin
Published: March 2020

A chemical study of the ethyl acetate (EtOAc) extract from the deep-sea-derived fungus Penicillium thomii YPGA3 led to the isolation of a new austalide meroterpenoid (1) and seven known analogues (2−8), two new labdane-type diterpenoids (9 and 10) and a known derivative (11). The structures of new compounds 1, 9, and 10 were determined by comprehensive analyses via nuclear magnetic resonance (NMR) and mass spectroscopy (MS) data. The absolute configurations of 1, 9, and 10 were determined by comparisons of experimental electronic circular dichroism (ECD) with the calculated ECD spectra. Compound 1 represented the third example of austalides bearing a hydroxyl group at C-5 instead of the conserved methoxy in other known analogues. To our knowledge, diterpenoids belonging to the labdane-type were discovered from species of Penicillium for the first time. Compound 1 showed cytotoxicity toward MDA-MB-468 cells with an IC50 value of 38.9 μM. Compounds 2 and 11 exhibited inhibition against α-glucosidase with IC50 values of 910 and 525 μM, respectively, being more active than the positive control acarbose (1.33 mM).
Primary Prevention of Cardiovascular Risk in Lithuania—Results from EUROASPIRE V Survey
Authors: Urbonas Gediminas , Vencevičienė Lina , Valius Leonas , Krivickienė Ieva , Petrauskas Linas , Lazarenkienė Gintarė , Karpavičienė Justina , Briedė Gabrielė , Žučenkienė Emilė , Vencevičius Karolis
Published: March 2020

Background and Objectives: Cardiovascular disease (CVD) prevention guidelines define targets for lifestyle and risk factors for patients at high risk of developing CVD. We assessed the control of these factors, as well as CVD risk perception in patients enrolled into the primary care arm of the European Action on Secondary and Primary Prevention by Intervention to Reduce Events (EUROASPIRE V) survey in Lithuania. Materials and Methods: Data were collected as the part of the EUROASPIRE V survey, a multicenter, prospective, cross-sectional observational study. Adults without a documented CVD who had been prescribed antihypertensive medicines and/or lipid-lowering medicines and/or treatment for diabetes (diet and/oral antidiabetic medicines and/or insulin) were eligible for the survey. Data were collected through the review of medical records, patients’ interview, physical examination and laboratory tests. Results: A total of 201 patients were enrolled. Very few patients reached targets for low-density lipoprotein cholesterol (LDL-C) (4.5%), waist circumference (17.4%) and body mass index (15.4%). Only 31% of very high CVD risk patients and 52% of high-risk patients used statins. Blood pressure target was achieved by 115 (57.2%) patients. Only 21.7% of patients at very high actual CVD risk and 27% patients at high risk correctly estimated their risk. Of patients at moderate actual CVD risk, 37.5% patients accurately self-assessed the risk. About 60%–80% of patients reported efforts to reduce the intake of sugar, salt or alcohol; more than 70% of patients were current nonsmokers. Only a third of patients reported weight reduction efforts (33.3%) or regular physical activity (27.4%). Conclusions: The control of cardiovascular risk factors in a selected group of primary prevention patients was unsatisfactory, especially in terms of LDL-C level and body weight parameters. Many patients did not accurately perceive their own risk of developing CVD.
Preparation and Mechanical Properties of ZK61-Y Magnesium Alloy Wheel Hub via Liquid Forging—Isothermal Forging Process
Authors: Qi Yushi , Wang Heng , Chen Lili , Zhang Hongming , Chen Gang , Chen Lihua , Du Zhiming
Published: March 2020

A ZK61-Y magnesium (Mg) alloy wheel hub was prepared via liquid forging—isothermal forging process. The effects of Y-element contents on the microstructure and mechanical properties of liquid forging blanks were investigated. The formation order of the second phase was I-phase (Mg3Zn6Y) → W-phase (Mg3Zn3Y2) → Z-phase (Mg12ZnY) with the increase of the Y-element content. Meanwhile, the I-phase and Z-phase formed in the liquid forging process were beneficial to the grain refinement. The numerical simulation of the isothermal forging process was carried out to analyze the effects of forming temperature on the temperature and stress field in the forming parts using the software Deform-3D. Isothermal forging experiments and post heat treatments were conducted. The influence of isothermal forging temperature, heat treatment temperature and preservation time on the microstructure and mechanical properties of the forming parts were also studied. The dynamic recrystallization (DRX), second-phase hardening, and work hardening account for the improvement of properties after the isothermal forging process. The forming part forged at 380 °C displayed the outstanding properties. The elongation, yield strength, and ultimate tensile strength were 18.5%, 150 MPa and 315 MPa, respectively. The samples displayed an increased elongation and decreased strength after heat treatments. The 520 °C—1 h sample possessed the best mechanical properties, the elongation was 25.5%, the yield stress was 125 MPa and the ultimate tensile strength was 282 MPa. This can be ascribed to the recrystallization and the elimination of working hardening. Meanwhile, the second phase transformation (I-phase → W-phase → Mg2Y + MgZn2), dissolution, and decomposition can be observed, as well.
Metabolic Phenotypes of Hypoxic-Ischemic Encephalopathy with Normal vs. Pathologic Magnetic Resonance Imaging Outcomes
Authors: Piñeiro-Ramos José David , Núñez-Ramiro Antonio , Llorens-Salvador Roberto , Parra-Llorca Anna , Sánchez-Illana Ángel , Quintás Guillermo , Boronat-González Nuria , Martínez-Rodilla Juan , Kuligowski Julia , Vento Máximo ,   The HYPOTOP Study Group
Published: March 2020

Hypoxic-Ischemic Encephalopathy (HIE) is one of the most relevant contributors to neurological disability in term infants. We hypothesized that clinical outcomes of newborns with (HIE) can be associated with changes at plasma metabolic level enabling the detection of brain injury. Plasma samples of a cohort of 55 asphyxiated infants who evolved to moderate/severe HIE were collected between birth and completion of therapeutic hypothermia (TH). Samples were analyzed employing a quantitative gas chromatography–mass spectrometry method for the determination of lactate and pyruvate and an untargeted liquid chromatography–time-of-flight mass spectrometry method for metabolic fingerprinting. Brain injury was assessed employing magnetic resonance imaging (MRI). A critical assessment of the usefulness of lactate, pyruvate, and pyruvate/lactate for outcome prediction was carried out. Besides, metabolic fingerprinting identified a dynamic perturbation of eleven metabolic pathways, including amino acid and purine metabolism, and the steroid hormone biosynthesis, in newborns with pathologic MRI outcomes. Although data suggest the usefulness of lactate and pyruvate monitoring during 72 h for discerning outcomes, only the steroid hormone biosynthesis pathway was significantly altered in early plasma samples (i.e., before the initiation of TH). This study highlights pathways that might potentially be targeted for biomarker discovery or adjuvant therapies to be combined with TH.
Color Mutations Alter the Biochemical Composition in the San Marzano Tomato Fruit
Authors: Dono Gabriella , Rambla Jose Luis , Frusciante Sarah , Granell Antonio , Diretto Gianfranco , Mazzucato Andrea
Published: March 2020

San Marzano (SM) is a traditional Italian landrace characterized by red elongated fruits, originating in the province of Naples (Italy) and cultivated worldwide. Three mutations, yellow flesh (r), green flesh (gf) and colorless fruit epidermis (y) were introduced into SM by backcross and the resulting introgression lines (ILs) produced the expected yellow, brown and pink fruit variants. In addition, ILs carrying double combinations of those mutations were obtained. The six ILs plus the SM reference were analyzed for volatile (VOC), non-polar (NP) and polar (P) metabolites. Sixty-eight VOCs were identified, and several differences evidenced in the ILs; overall gf showed epistasis over r and y and r over y. Analysis of the NP component identified 54 metabolites; variation in early carotenoids (up to lycopene) and chlorophylls characterized respectively the ILs containing r and gf. In addition, compounds belonging to the quinone and xanthophyll classes were present in genotypes carrying the r mutation at levels higher than SM. Finally, the analysis of 129 P metabolites evidenced different levels of vitamins, amino acids, lipids and phenylpropanoids in the ILs. A correlation network approach was used to investigate metabolite–metabolite relationships in the mutant lines. Altogether these differences potentially modified the hedonistic and nutritional value of the berry. In summary, single and combined mutations in gf, r and y generated interesting visual and compositional diversity in the SM landrace, while maintaining its original typology.
Salmonella Virulence and Immune Escape
Authors: Wang Mengyao , Qazi Izhar Hyder , Wang Linli , Zhou Guangbin , Han Hongbing
Published: March 2020

Salmonella genus represents the most common foodborne pathogens causing morbidity, mortality, and burden of disease in all regions of the world. The introduction of antimicrobial agents and Salmonella-specific phages has been considered as an effective intervention strategy to reduce Salmonella contamination. However, data from the United States, European countries, and low- and middle-income countries indicate that Salmonella cases are still a commonly encountered cause of bacterial foodborne diseases globally. The control programs have not been successful and even led to the emergence of some multidrug-resistant Salmonella strains. It is known that the host immune system is able to effectively prevent microbial invasion and eliminate microorganisms. However, Salmonella has evolved mechanisms of resisting host physical barriers and inhibiting subsequent activation of immune response through their virulence factors. There has been a high interest in understanding how Salmonella interacts with the host. Therefore, in the present review, we characterize the functions of Salmonella virulence genes and particularly focus on the mechanisms of immune escape in light of evidence from the emerging mainstream literature.
Differences in Gut Microbial Diversity are Driven by Drug Use and Drug Cessation by Either Compulsory Detention or Methadone Maintenance Treatment
Authors: Li Qiaoyan , Chen Siqi , Liu Ke , Long Danfeng , Liu Diru , Jing Zhengchao , Huang Xiaodan
Published: March 2020

In this work, we investigate differences in gut microbial diversity driven by drug use or by the widely used methods for drug cessation: methadone maintenance treatment (MMT) and compulsory detention (CD). Methods: 99 participants (28 CD participants, 16 MMT patients, 27 drug users, and 28 healthy controls) were selected using strict inclusion criteria. Nutritional intake and gut microbial diversity were analyzed with bioinformatics tools and SPSS 20.0. Results: Alpha diversity was not significantly different among groups, whereas beta diversity of gut microbiota and nutrient intake were significantly higher among MMT patients. Taxa were unevenly distributed between groups, with drug users having the highest proportion of Ruminococcus and MMT patients having the highest abundance of Bifidobacterium and Lactobacillus. Conclusion: Drug use, cessation method, and diet contribute to shaping human gut communities. High beta diversity among MMT patients is likely driven by methadone use and high nutrient intake, leading to increased orexin A and enrichment for beneficial bacteria, while diversity in CD participants is largely influenced by diet.
Plant Growth Promotion Abilities of Phylogenetically Diverse Mesorhizobium Strains: Effect in the Root Colonization and Development of Tomato Seedlings
Authors: Menéndez Esther , Pérez-Yépez Juan , Hernández Mercedes , Rodríguez-Pérez Ana , Velázquez Encarna , León-Barrios Milagros
Published: March 2020

Mesorhizobium contains species widely known as nitrogen-fixing bacteria with legumes, but their ability to promote the growth of non-legumes has been poorly studied. Here, we analyzed the production of indole acetic acid (IAA), siderophores and the solubilization of phosphate and potassium in a collection of 24 strains belonging to different Mesorhizobium species. All these strains produce IAA, 46% solubilized potassium, 33% solubilize phosphate and 17% produce siderophores. The highest production of IAA was found in the strains Mesorhizobium ciceri CCANP14 and Mesorhizobium tamadayense CCANP122, which were also able to solubilize potassium. Moreover, the strain CCANP14 showed the maximum phosphate solubilization index, and the strain CCANP122 was able to produce siderophores. These two strains were able to produce cellulases and cellulose and to originate biofilms in abiotic surfaces and tomato root surface. Tomato seedlings responded positively to the inoculation with these two strains, showing significantly higher plant growth traits than uninoculated seedlings. This is the first report about the potential of different Mesorhizobium species to promote the growth of a vegetable. Considering their use as safe for humans, animals and plants, they are an environmentally friendly alternative to chemical fertilizers for non-legume crops in the framework of sustainable agriculture.
Comparison of LAL and rFC Assays—Participation in a Proficiency Test Program between 2014 and 2019
Authors: Piehler Maike , Roeder Ruth , Blessing Sina , Reich Johannes
Published: March 2020

Endotoxin (lipopolysaccharide) testing of drugs is routinely required in pharmaceutical industries. Suitable compendial assays are defined by national pharmacopoeias. At this time, Limulus Amoebocyte Lysate (LAL) assays are the gold standard. LAL is used in vitro for specific detection of endotoxin based on endotoxin-activated Factor C-mediated clotting cascade. However, alternative mediated pathways (e.g., Factor G), impurities, and further factors may influence test results. Some of these influencing factors are eliminated by recombinant Factor C (rFC) test, which represents a promising alternative. rFC not only enables highly specific endotoxin testing, as interfering Horseshoe Crab blood components are eliminated, but also offers ethical and ecological advantages compared to classical LAL assays. However, the question remains whether rFC-based tests are robust test systems, equivalent or superior to LAL and suitable for routine bacterial endotoxin testing. Pharmaceutical test users have validated the test successfully for their specific products, but no long-term studies have been published that combine testing of unknown samples, inter-laboratory, -operator, and -lot changes. Thus, it was of great interest to investigate rFC test performance in a routine setting within a proficiency test program set-up. Over a period of six years comparative endotoxin testing was conducted with one kinetic chromogenic LAL assay and two rFC-based assays. Results of this study demonstrate that both rFC-based assays were comparable to LAL. All results met acceptance criteria defined by compendial bacterial endotoxin testing. RFC-based methods generated results with even better endotoxin recovery rates compared to LAL. Therefore, rFC-based tests were found to represent reliable methods, as equivalent or even superior to LAL assays and suitable for routine bacterial endotoxin testing.
Yeasts and Bacterial Consortia from Kefir Grains Are Effective Biocontrol Agents of Postharvest Diseases of Fruits
Authors: Zhimo V. Yeka , Biasi Antonio , Kumar Ajay , Feygenberg Oleg , Salim Shoshana , Vero Silvana , Wisniewski Michael , Droby Samir
Published: March 2020

Fungal pathogens in fruits and vegetables cause significant losses during handling, transportation, and storage. Biological control with microbial antagonists replacing the use of chemical fungicides is a major approach in postharvest disease control, and several products based on single antagonists have been developed but have limitations related to reduced and inconsistent performance under commercial conditions. One possible approach to enhance the biocontrol efficacy is to broaden the spectrum of the antagonistic action by employing compatible microbial consortia. Here, we explore commercial kefir grains, a natural probiotic microbial consortium, by culture-dependent and metagenomic approaches and observed a rich diversity of co-existing yeasts and bacterial population. We report effective inhibition of the postharvest pathogen Penicillium expansum on apple by using the grains in its fresh commercial and milk-activated forms. We observed few candidate bacteria and yeasts from the kefir grains that grew together over successive enrichment cycles, and these mixed fermentation cultures showed enhanced biocontrol activities as compared to the fresh commercial or milk-activated grains. We also report several individual species of bacteria and yeasts with biocontrol activities against Penicillium rots on apple and grapefruit. These species with antagonistic properties could be further exploited to develop a synthetic consortium to achieve enhanced antagonistic effects against a wide range of postharvest pathogens.
Small Sample Stress: Probing Oxygen-Deprived Ammonia-Oxidizing Bacteria with Raman Spectroscopy In Vivo
Authors: Kniggendorf Ann-Kathrin , Nogueira Regina , Nasiri Bahmanabad Somayeh , Pommerening-Röser Andreas , Roth Bernhard Wilhelm
Published: March 2020

The stress response of ammonia-oxidizing bacteria (AOB) to oxygen deprivation limits AOB growth and leads to different nitrification pathways that cause the release of greenhouse gases. Measuring the stress response of AOB has proven to be a challenge due to the low growth rates of stressed AOB, making the sample volumes required to monitor the internal stress response of AOB prohibitive to repeated analysis. In a proof-of-concept study, confocal Raman microscopy with excitation resonant to the heme c moiety of cytochrome c was used to compare the cytochrome c content and activity of stressed and unstressed Nitrosomonas europaea (Nm 50), Nitrosomonas eutropha (Nm 57), Nitrosospira briensis (Nsp 10), and Nitrosospira sp. (Nsp 02) in vivo. Each analysis required no more than 1000 individual cells per sampling; thus, the monitoring of cultures with low cell concentrations was possible. The identified spectral marker delivered reproducible results within the signal-to-noise ratio of the underlying Raman spectra. Cytochrome c content was found to be elevated in oxygen-deprived and previously oxygen-deprived samples. In addition, cells with predominantly ferrous cytochrome c content were found in deprived Nitrosomonas eutropha and Nitrosospira samples, which may be indicative of ongoing electron storage at the time of measurement.