bluuebunny/crossref_metadata_2025 huggingface dataset:
The Good Treatment, the Bad Virus, and the Ugly Inflammation: Pathophysiology of Kidney Involvement During COVID-19
Authors: Le Stang Marie-Bénédicte , Desenclos Jordan , Flamant Martin , Chousterman Benjamin G. , Tabibzadeh Nahid
Published: March 2021

Kidney involvement is a common complication during SARS-CoV-2 infection. Its association with poor outcomes, especially in critically ill patients, raises issues whether kidney involvement reflects multi-organ damage or if it is a specific feature of the infection. Based on observational studies, autopsy series, and on current understanding of the route of entry of the virus, this review will highlight the different types of kidney involvement during COVID-19 and put them in the perspective of the different pathophysiological hypotheses. Virus entry route through ACE2 ligation and TMPRSS2 coligation allows identifying potential viral targets in the kidney, including tubules, endothelial cells, and glomerulus. While reports have described damages of all these structures and virus kidney tropism has been identified in renal extracts in autopsy series, no direct viral infection has been found in the latter structures thus far on kidney biopsies. Notwithstanding the technical challenge of disclosing viral invasion within tissues and cells, viral direct cytopathogenic effect generally does not appear as the cause of the observed renal damage. Inflammation and altered hemodynamics, described as “viral sepsis,” might rather be responsible for organ dysfunction, including kidneys. We shall place these various mechanisms into an integrated vision where the synergy between direct viral pathogenicity and systemic inflammation enhances renal damage. As SARS-CoV-2 inexorably continues its rampant spread, understanding the sequence of events in the kidneys might thus help inform improved therapeutic strategies, including antiviral drugs and immunomodulators.
Exploring Relationships Between Anthropometry, Body Composition, Maturation, and Selection for Competition: A Study in Youth Soccer Players
Authors: Clemente Filipe Manuel , Clark Cain C. T. , Leão César , Silva Ana Filipa , Lima Ricardo , Sarmento Hugo , Figueiredo António J. , Rosemann Thomas , Knechtle Beat
Published: March 2021

Purpose

The purpose of this study was to analyze variations of selection for competition between late and early mature players and test the relationships between anthropometry, body composition, maturation, and selection for competition.

Methods

Seventy-nine youth soccer players from under-11 to under-14 participated in this study, over 6 months. Body composition and maturity offset were estimated based on anthropometric data collected. Participants were also monitored for their number of matches as starters and time of play accrued in minutes.

Results

Minutes played had large correlation coefficients with maturity offset (r = 0.58), and leg length and sitting height interaction (r = 0.56). Multiple linear regression explained 35% of the variation in minutes played (p < 0.001, R2 = 0.41, R2adjusted = 0.35, RMSE = 334.8), but only 12% of the variation in matches as starter (p = 0.04, R2 = 0.21, R2adjusted = 0.12, RMSE = 5.47) between above and below the median of the maturity offset was accounted for, respectively.

Conclusions

Although maturation may play a role in the minutes of play accrued and matches as starters in young, it is not necessarily determining. A significant amount of the variation in the minutes of play accrued of players can be accounted for when considering body composition and anthropometric data.
Cadmium-Tolerant and -Sensitive Cultivars Identified by Screening of Medicago truncatula Germplasm Display Contrasting Responses to Cadmium Stress
Authors: García de la Torre Vanesa S. , Coba de la Peña Teodoro , Pueyo José J. , Lucas M. Mercedes
Published: March 2021

Cadmium (Cd) pollution in soils is an increasing problem worldwide, and it affects crop production and safety. We identified Cd-tolerant and -sensitive cultivars by testing 258 accessions of Medicago truncatula at seedling stage, using the relative root growth (RRG) as an indicator of Cd tolerance. The factorial analysis (principal component analysis method) of the different growth parameters analyzed revealed a clear differentiation between accessions depending on the trait (tolerant or sensitive). We obtained a normalized index of Cd tolerance, which further supported the suitability of RRG to assess Cd tolerance at seedling stage. Cd and elements contents were analyzed, but no correlations with the tolerance trait were found. The responses to Cd stress of two accessions which had similar growth in the absence of Cd, different sensitivity to the metal but similar Cd accumulation capacity, were analyzed during germination, seedling stage, and in mature plants. The results showed that the Cd-tolerant accession (CdT) displayed a higher tolerance than the sensitive cultivar (CdS) in all the studied stages. The increased gene expression of the three main NADPH recycling enzymes in CdT might be key for this tolerance. In CdS, Cd stress produced strong expression of most of the genes that encode enzymes involved in glutathione and phytochelatin biosynthesis (MtCYS, MtγECS, and MtGSHS), as well as GR, but it was not enough to avoid a redox status imbalance and oxidative damages. Our results on gene expression, enzyme activity, antioxidant content, and lipid peroxidation indicate different strategies to cope with Cd stress between CdS and CdT, and provide new insights on Cd tolerance and Cd toxicity mechanisms in M. truncatula.
Lewis A Glycans Are Present on Proteins Involved in Cell Wall Biosynthesis and Appear Evolutionarily Conserved Among Natural Arabidopsis thaliana Accessions
Authors: Beihammer Gernot , Maresch Daniel , Altmann Friedrich , Van Damme Els J. M. , Strasser Richard
Published: March 2021

N-glycosylation is a highly abundant protein modification present in all domains of life. Terminal sugar residues on complex-type N-glycans mediate various crucial biological processes in mammals such as cell-cell recognition or protein-ligand interactions. In plants, the Lewis A trisaccharide constitutes the only known outer-chain elongation of complex N-glycans. Lewis A containing complex N-glycans appear evolutionary conserved, having been identified in all plant species analyzed so far. Despite their ubiquitous occurrence, the biological function of this complex N-glycan modification is currently unknown. Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa. Affinity purification via the JIM84 antibody, directed against Lewis A structures on complex plant N-glycans, was used to enrich Lewis A bearing glycoproteins, which were subsequently identified via nano-LC-MS. Selected identified proteins were recombinantly expressed and the presence of Lewis A confirmed via immunoblotting and site-specific N-glycan analysis. While the proteins identified in O. sativa are associated with diverse functions, proteins from A. thaliana and N. benthamiana are mainly involved in cell wall biosynthesis. However, a Lewis A-deficient mutant line of A. thaliana showed no change in abundance of cell wall constituents such as cellulose or lignin. Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis. Besides one relict line showing no detectable levels of Lewis A, the modification was present in all other tested accessions. The data provided here comprises the so far first attempt at identifying Lewis A bearing glycoproteins across different species and will help to shed more light on the role of Lewis A structures in plants.
Arbuscular Mycorrhiza Improves Photosynthesis and Restores Alteration in Sugar Metabolism in Triticum aestivum L. Grown in Arsenic Contaminated Soil
Authors: Gupta Samta , Thokchom Sarda Devi , Kapoor Rupam
Published: March 2021

Contamination of agricultural soil by arsenic (As) is a serious menace to environmental safety and global food security. Symbiotic plant–microbe interaction, such as arbuscular mycorrhiza (AM), is a promising approach to minimize hazards of As contamination in agricultural soil. Even though the potential of AM fungi (AMF) in redeeming As tolerance and improving growth is well recognized, the detailed metabolic and physiological mechanisms behind such beneficial effects are far from being completely unraveled. The present study investigated the ability of an AM fungus, Rhizophagus intraradices, in mitigating As-mediated negative effects on photosynthesis and sugar metabolism in wheat (Triticum aestivum) subjected to three levels of As, viz., 0, 25, and 50 mg As kg–1 of soil, supplied as sodium arsenate. As exposure caused significant decrease in photosynthetic pigments, Hill reaction activity, and gas exchange parameters such as net photosynthetic rate, stomatal conductance, transpiration rate, and intercellular CO2 concentration. In addition, As exposure also altered the activities of starch-hydrolyzing, sucrose-synthesizing, and sucrose-degrading enzymes in leaves. Colonization by R. intraradices not only promoted plant growth but also restored As-mediated impairments in plant physiology. The symbiosis augmented the concentration of photosynthetic pigments, enhanced Hill reaction activity, and improved leaf gas exchange parameters and water use efficiency of T. aestivum even at high dose of 50 mg As kg–1 of soil. Furthermore, inoculation with R. intraradices also restored As-mediated alteration in sugar metabolism by modulating the activities of starch phosphorylase, α-amylase, β-amylase, acid invertase, sucrose synthase, and sucrose-phosphate synthase in leaves. This ensured improved sugar and starch levels in mycorrhizal plants. Overall, the study advocates the potential of R. intraradices in bio-amelioration of As-induced physiological disturbances in wheat plant.
Measuring Online Wellbeing: A Scoping Review of Subjective Wellbeing Measures
Authors: Ong Zhen Xin , Dowthwaite Liz , Perez Vallejos Elvira , Rawsthorne Mat , Long Yunfei
Published: March 2021

With the increasing importance of the internet to our everyday lives, questions are rightly being asked about how its' use affects our wellbeing. It is important to be able to effectively measure the effects of the online context, as it allows us to assess the impact of specific online contexts on wellbeing that may not apply to offline wellbeing. This paper describes a scoping review of English language, peer-reviewed articles published in MEDLINE, EMBASE, and PsychInfo between 1st January 2015 and 31st December 2019 to identify what measures are used to assess subjective wellbeing and in particular to identify any measures used in the online context. Two hundred forty studies were identified; 160 studies were removed by abstract screening, and 17 studies were removed by full-text screening, leaving 63 included studies. Fifty-six subjective wellbeing scales were identified with 18 excluded and 38 included for further analysis. Only one study was identified researching online wellbeing, and no specific online wellbeing scale was found. Therefore, common features of the existing scales, such as the number and type of questions, are compared to offer recommendations for building an online wellbeing scale. Such a scale is recommended to be between 3 and 20 questions, using mainly 5-point Likert or Likert-like scales to measure at least positive and negative affect, and ideally life satisfaction, and to use mainly subjective evaluation. Further research is needed to establish how these findings for the offline world effectively translate into an online measure of wellbeing.
Consistency of Parental and Self-Reported Adolescent Wellbeing: Evidence From Developmental Language Disorder
Authors: Gough Kenyon Sheila M. , Palikara Olympia , Lucas Rebecca M.
Published: March 2021

Research on adolescent wellbeing in Developmental Language Disorder (DLD) has previously been examined through measures of parent (proxy) or self-reported wellbeing, but never has a study included both and enabled comparison between the two. The current study reports parent and self rated wellbeing of adolescents with DLD and Low Language (LL) ability, as well as their typically developing (TD) peers. It also examines consistency between raters and factors influencing correspondence. Adolescents aged 10–11 with DLD (n= 30), LL (n= 29) or TD (n= 48) were recruited from eight UK primary schools. A battery of standardized language, psychosocial and wellbeing assessments, including the KIDSCREEN-27 were administered. Adolescent ratings of wellbeing were similar across groups on three of the five wellbeing dimensions, but those with DLD had lower self-reported Autonomy and Parental Relations than their TD peers, and both the DLD and LL group had lower School Environment scores than their TD peers. By parental report, the DLD and LL group were considered to have lower wellbeing on all five wellbeing dimensions relative to their TD peers. Paired samplet-test analyses indicated a high level of variance between parent and adolescent reported wellbeing for multiple wellbeing domains, especially Psychological Wellbeing. Importantly, predictors of the level of agreement between parent and adolescent reported psychological wellbeing differed between groups: cognitive reappraisal and sociability predicted this level of agreement for adolescents with LL, while social competence predicted agreement in DLD and TD. This study emphasizes the necessity of allowing adolescents of all language abilities to report their own wellbeing, as their perspective does not align with that of their parents. It also highlights the importance of including the full spectrum of need when investigating the impact of language ability on consistency between proxy and self-reported wellbeing.
Nudging Commuters to Increase Public Transport Use: A Field Experiment in Rotterdam
Authors: Franssens Samuel , Botchway Ebo , de Swart Willie , Dewitte Siegfried
Published: March 2021

A large-scale field experiment in Rotterdam, Netherlands, tested whether nudging could increase public transport use. During one work week, 4000 commuters on six bus lines, received a free travel card holder. On the three bus lines in the experimental condition, the card holders displayed a social label that branded bus passengers as sustainable travelers because of their bus use. On the three bus lines in the control condition, there was no such message on the card holders. Analysis of the number of rides per hour showed that the intervention led to a change from pre-intervention (619 days) to post-intervention period (176 days) that was estimated to be 1.18 rides per day greater on experimental lines than on control lines. This experiment shows that public transport operators can increase public transport use by incorporating messages that positively label passengers as sustainable travelers in their communication strategies.
Assessing the Importance of Psychosocial Factors Associated With Sustainable Organizational Development During COVID-19
Authors: Dragan Florin , Luo Chaoping , Ivascu Larisa , Ali Majid
Published: March 2021

Involvement in sustainable development is a voluntary activity. Organizations apply the principles of sustainable development only when they identify several benefits. These benefits are identified, especially with the financial ones. The involvement of organizations in sustainable organizations has different intensity levels. These intensity levels are influenced by psychosocial factors (PF), attitudes toward organizational risks, and organizational and urban policies. The present paper identifies the key psychological factors involved in applying organizational sustainability principles within organizations. For this research, five groups were created for in-depth interviews with key people from Romania’s innovative companies. To identify the importance of the framework analysis factors, the Delphi method was used, in which 20 experts from different fields of activity were involved. Following the rounds involved in the Delphi method, the ranking of PF on four levels of importance was accepted, based on planned behavior and reasoned action theory. These levels were correlated with the intensity levels of involvement in sustainable development. The entire market study was conducted during COVID-19, which significantly impacted specific directions. As a result, it could be observed that motivation, learning attitude, behavior, and intention to take precedence are essential in the organizational sustainability approach.
Implementation and Evaluation of a Social Media-Based Communication Strategy to Enhance Employee Engagement: Experiences From a Children's Hospital, Pakistan
Authors: Rasheed Muneera A. , Hookmani Alma Arshad , Waleed Sana , Fatima H. Sundus , Siddiqui Soha , Khurram Muhammad , Hasan Babar S.
Published: March 2021

Social media can complement organizational communication strategy which is integral to employee engagement. However, successful case studies which can allow replication are limited. The objective of the study is to describe the design, implementation, and evaluation of a social media-based communication strategy in a tertiary care hospital in Pakistan. The leadership of the pediatric service line developed an intervention plan to engage the employees with the newly reframed vision to improve patient and family experience. An online communication platform—Facebook page—was created for all employees of the pediatric service line. The strategy to influence employees was based on Cialdini's six principles of persuasion. Implementation of the strategy between October 2017 and December 2019 was evaluated for reach, discussion themes, and outcomes using the framework by Murdough (2009). Quantitative indicators included total posts, mean comments, and reactions per post. Posts were qualitatively analyzed with an emergent approach for insights into the discussion. The analysis revealed a total of 9,085 posts, with mean reactions per post of 8.4, mean comments of 7.2, and active viewership by 90% members on average. In terms of post types, photos were the highest (4,779), while videos were the lowest (1,163). Qualitative analysis indicated 54% of the posts were of the theme “inspirational and thought provoking,” while the greatest engagement was generated on the theme “challenges and solution.” The authors conclude that the strategy was successfully implemented to maintain active membership, engage employees in meaningful conversations, and have them express intent to execute quality improvement projects.
The Impact of the Coronavirus Pandemic on Supply Chains and Their Sustainability: A Text Mining Approach
Authors: Meyer Amelie , Walter Wiebke , Seuring Stefan
Published: March 2021

The coronavirus pandemic is an unprecedented event, putting global supply chains (SCs) into the focus of a wider public. Yet it is unclear what is communicated about this and how and what consequences SC management (SCM) would take away. This research aims at analyzing how text mining can provide insights on the impact of the coronavirus pandemic on SCs, focusing on the implications of the pandemic for the SC constructs related to risk, resilience, and sustainability. A method applies text mining of general newspapers as well as SC and logistic newspaper articles employing the open-source software R. This paper shows that certain SC topics like risk, resilience, disruption, and sustainability vary in their news coverage on the type of newspaper and the number of coronavirus disease 2019 (COVID-19) infections. It reassures trends and observations from individual experiences on a broader and global picture and discusses the limitations and possibilities of using text mining in this field. The time period was split into three phases regarding the course of the number of infections and differences in the news coverage of the phases that can be distinguished already: (1) the onset of the crisis, (2) the peak and lockdown, and (3) managing SCs during the crisis. As this pandemic is highly dynamic, is new, and has not yet ended, research implications are too early to make. This research rather serves as a base for further, more detailed research into certain topics and identifies limitations and improvements of the applied method. Due to the method chosen and the timeliness of data, this empirical research is unique and can be of great help to see trends and patterns to further deep dive into specific areas.
Freeride Skiing—Risk-Taking, Recognition, and Moral Boundaries
Authors: Tøstesen Gustav , Langseth Tommy
Published: March 2021

Freeride skiing is an activity that is, or at least can be, quite dangerous. Risk-taking in high-risk sports has usually been understood within a psychological framework. Building on Pierre Bourdieu's sociology, this article highlights the social dimension of risk-taking in freeride skiing by scrutinizing values within a freeride culture. A central question in this article is: what kind of actions are given recognition and credibility in freeride skiing? The findings show that there is a clear link between risk-taking and credibility and that risk-taking might be seen as a form of capital. However, risk-taking's link to recognition is not straightforward—it is limited by the skiers' skill level. To further develop our understanding of the social dimension of risk-taking we use Michelle Lamont's theory of symbolic boundaries. By expanding the Bourdieusian understanding of social practice with Lamont's work, we gain insight into how risk-taking is socially regulated by social conventions within a subculture. This means that we in this article describe three social dimensions of risk-taking: (1) The link between risk-taking and recognition, (2) The limits of the risk-recognition nexus, and (3) The moral boundaries of risk-taking.
Mono-Vision Based Lateral Localization System of Low-Cost Autonomous Vehicles Using Deep Learning Curb Detection
Authors: Yu Junwei , Yu Zhuoping
Published: March 2021

The localization system of low-cost autonomous vehicles such as autonomous sweeper requires a highly lateral localization accuracy as the vehicle needs to keep a near lateral-distance between the side brush system and the road curb. Existing methods usually rely on a global navigation satellite system that often loses signal in a cluttered environment such as sweeping streets between high buildings and trees. In a GPS-denied environment, map-based methods are often used such as visual and LiDAR odometry systems. Apart from heavy computation costs from feature extractions, they are too expensive to meet the low-price market of the low-cost autonomous vehicles. To address these issues, we propose a mono-vision based lateral localization system of an autonomous sweeper. Our system relies on a fish-eye camera and precisely detects road curbs with a deep curb detection network. Curbs locations are then referred to as straightforward marks to control the lateral motion of the vehicle. With our self-recorded dataset, our curb detection network achieves 93% pixel-level precision. In addition, experiments are performed with an intelligent sweeper to prove the accuracy and robustness of our proposed approach. Results demonstrate that the average lateral distance error and the maximum invalid rate are within 0.035 m and 9.2%, respectively.
Swine Inflammation and Necrosis Syndrome Is Associated with Plasma Metabolites and Liver Transcriptome in Affected Piglets
Authors: Ringseis Robert , Gessner Denise K. , Loewenstein Frederik , Kuehling Josef , Becker Sabrina , Willems Hermann , Lechner Mirjam , Eder Klaus , Reiner Gerald
Published: March 2021

Swine Inflammation and Necrosis Syndrome can lead to severe clinical signs, especially in tails, ears, teats, and claws in pigs. Clinical and histopathological findings in newborn piglets with intact epidermis indicate a primarily endogenous etiology, and microbial-associated molecular patterns (MAMPs), such as lipopolysaccharide (LPS) are assumed to play a central role in the development of the syndrome. We hypothesized that swine inflammation and necrosis syndrome (SINS) is indirectly triggered by gut-derived MAMPs entering the circulatory system via the liver and thereby causing derangements on liver metabolism. To test this hypothesis, metabolomes, candidate genes of the liver and liver transcriptomes of 6 piglets with high-grade clinical signs of SINS (SINS high) were examined and compared with 6 piglets without significant signs of SINS (SINS low). Several hepatic pro-inflammatory genes and genes involved in stress response were induced in piglets of the SINS high group. The most striking finding from hepatic transcript profiling and bioinformatic enrichment was that the most enriched biological processes associated with the approximately 220 genes induced in the liver of the SINS high group were exclusively related to metabolic pathways, such as fatty acid metabolic process. Within the genes (≈390) repressed in the liver of the SINS high group, enriched pathways were ribosome biogenesis, RNA processing, RNA splicing, spliceosome, and RNA transport. The transcriptomic findings were supported by the results of the metabolome analyses. These results provide the first evidence for the induction of an inflammatory process in the liver of piglets suffering from SINS, accompanied by lipid metabolic derangement.
Relation between Feed Particle Size Distribution and Plumage Condition in Laying Hens on Commercial Farms
Authors: Schreiter Ruben , Damme Klaus , Freick Markus
Published: March 2021

In this cross-sectional study, 103 complete feed samples from laying hen herds affected by plumage damage as an indirect measure for severe feather pecking (affected herds; AH, n = 37) and control herds without plumage damage (control herd; CH, n = 66) of commercial German farms were examined by dry sieve and nutrient analysis. AH showed higher percentages of particles >2.50 mm (mean ± SD, CH: 11.0 ± 8.5%, AH: 24.9 ± 14.3%) and 2.00–2.50 mm (CH: 11.2 ± 5.3%, AH: 15.7 ± 5.7%), but lower proportions of fractions 1.01–1.60 mm (CH: 22.9 ± 4.9%, AH: 17.8 ± 5.7%), 0.51–1.00 mm (CH: 25.5 ± 8.2%, AH: 16.0 ± 6.8%) and ≤0.50 mm (CH: 15.4 ± 5.0%, AH: 11.0 ± 4.8%) (p < 0.001). Diets of AH had a higher geometric mean diameter (GMD) compared to CH (AH: 1470.8 ± 343.9 μm; CH: 1113.3 ± 225.7 μm) (p < 0.001). Contents of crude ash (CH: 130.3 ± 18.8 g/kg, AH: 115.9 ± 24.3 g/kg), lysine (CH: 8.2 ± 1.0 g/kg, AH: 7.7 ± 1.2 g/kg), methionine (CH: 3.4 ± 0.5 g/kg, AH: 3.2 ± 0.6 g/kg) and sodium (CH: 1.7 ± 0.4 g/kg, AH: 1.3 ± 0.4 g/kg) were lower in AH (p ≤ 0.041). In a logistic regression model, animal age (p = 0.041) and GMD (p < 0.001) were significant factors on the occurrence of plumage damage.
Laterality in Responses to Acoustic Stimuli in Giant Pandas
Authors: Liu He , Tang Yezhong , Ni Yanxia , Fang Guangzhan
Published: March 2021

Cerebral lateralization is a common feature present in many vertebrates and is often observed in response to various sensory stimuli. Numerous studies have proposed that some vertebrate species have a right hemisphere or left hemisphere dominance in response to specific types of acoustic stimuli. We investigated lateralization of eight giant pandas (Ailuropoda melanoleuca) by using a head turning paradigm and twenty-eight acoustic stimuli with different emotional valences which included twenty-four conspecific and four non-conspecific acoustic stimuli (white noise, thunder, and vocalization of a predator). There was no significant difference in auditory laterality in responses to conspecific or non-conspecific sounds. However, the left cerebral hemisphere processed the positive stimuli, whereas neither of the two hemispheres exhibited a preference for processing the negative stimuli. Furthermore, the right hemisphere was faster than the left hemisphere in processing emotional stimuli and conspecific stimuli. These findings demonstrate that giant pandas exhibit lateralization in response to different acoustic stimuli, which provides evidence of hemispheric asymmetry in this species.
Effect of a Phytogenic Feed Additive on Growth Performance, Nutrient Digestion, and Immune Response in Broiler-Fed Diets with Two Different Levels of Crude Protein
Authors: Wang Jinquan , Su Shengchen , Pender Chasity , Murugesan Raj , Syed Basharat , Kim Woo Kyun
Published: March 2021

The aim of this experiment was to evaluate the effect of a phytogenic feed additive (PFA) on growth performance and nutrient digestibility of broilers fed corn and soybean meal-based diets containing two different levels of crude protein. A 2 × 2 completely randomized factorial arrangement (eight replicates/treatment, 30 birds/replicate) was conducted with a positive control (PC) and negative control (NC) containing crude protein at standard or reduced by 1.5% (equivalent to a reduction of 15 g/kg), respectively, and supplementation of PFA at 0 or 125 ppm of diet. There were no significant interactions found between PFA and CP levels in the current study. Main effect analysis showed that during 0–42 d of age NC diets decreased body weight gain (p < 0.05), but increased feed intake (p < 0.05) and feed conversion ratio (FCR, p < 0.01), whereas supplementation of PFA resulted in a lower FCR (p < 0.01). The ileal nutrient digestibility was reduced (p < 0.05) in the broilers fed a reduced protein diet at 21 d compared to the standard protein level group, but there were no effects for PFA levels. Similarly, supplementing PFAs showed no effects on digestive enzyme (Alkaline phosphatase, amylase, and lipase) activity in jejunal digesta and jejunal brush border enzyme (maltase, sucrase, and aminopeptidase) activity. Supplementation of PFA downregulated (p < 0.05) the mRNA expressions of cytochrome P450 1A and interleukin 6 in the ileum but had no effects on nutrient transporter genes in the jejunum. In conclusion, supplementation of PFA reduced broiler FCR during the whole grow-out period and positively regulated the immune responses in the ileum.
Exploratory Study: Excessive Iron Supplementation Reduces Zinc Content in Pork without Affecting Iron and Copper
Authors: Middleton Maureen , Olivares Manuel , Espinoza Alejandra , Arredondo Miguel , Pizarro Fernando , Valenzuela Carolina
Published: March 2021

The aim of this work was to determine in an exploratory manner the effect of excessive iron supplementation on iron, zinc, and copper contents in pork and pork offal. Pigs averaging 50 days in age and 15 ± 1.3 kg body weight were allocated to a control group (500 ppm dietary Fe) and a supplemental group (3000 ppm dietary Fe). After an iron supplementation period of 60 days, blood samples were analyzed to determine iron biomarkers, serum copper, and zinc contents. Animals were slaughtered to assess total iron, non-heme iron, heme iron, zinc, and copper contents in samples of nine meat cuts and some offal. Iron supplementation improved the iron status in pigs with increased hemoglobin and hematocrit, but did not affect serum levels of iron, zinc, and copper. Iron supplementation did not affect the heme and non-heme iron contents of the different meat cuts. Zinc contents decreased by 32–55% in meat cuts, where iron content increased in the liver, spleen, kidneys, and pancreas. No differences of zinc and copper were observed in offal samples. High concentrations of iron supplementation reduce zinc content in pork.
Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Isolates from Northern Africa and the Middle East
Authors: Higgins Paul G. , Hagen Ralf Matthias , Kreikemeyer Bernd , Warnke Philipp , Podbielski Andreas , Frickmann Hagen , Loderstädt Ulrike
Published: March 2021

At the Bundeswehr Hospitals of Hamburg and Westerstede, patients repatriated from subtropical war and crisis zones of Northern Africa and the Middle East were medically treated, including microbiological assessment. Within a six-year interval, 16 Acinetobacter spp. strains, including 14 Acinetobacter baumannii (Ab) isolates with resistance against carbapenems and origins in Afghanistan (n = 4), Iraq (n = 2), Libya (n = 2), and Syria (n = 8) were collected. While clonal relationships of Libyan and Syrian strains had been assessed by superficial next generation sequencing (NGS) and “DiversiLab” repetitive elements sequence-based (rep-)PCR so far, this study provides core genome-based sequence typing and thus more detailed epidemiological information. In detail, sequencing allowed a definitive species identification and comparison with international outbreak-associated Ab strains by core genome multi locus sequence typing (cgMLST) and the identification of MLST lineages, as well as the identification of known resistance genes. The sequence analysis allowed for the confirmation of outbreak-associated clonal clusters among the Syrian and Afghan Ab isolates, indicating likely transmission events. The identified acquired carbapenem resistance genes comprised blaOXA-23, blaOXA-58, blaNDM-1, and blaGES-11, next to other intrinsic and acquired, partly mobile resistance-associated genes. Eleven out of 14 Ab isolates clustered with the previously described international clonal lineages IC1 (4 Afghan strains), IC2 (6 Syrian strains), and IC7 (1 Syrian strain). Identified Pasteur sequence types of the 14 Ab strains comprised ST2 (Syrian), ST25 (Libyan), ST32 (Iraqi), ST81 (Afghan), ST85 (Libyan), and ST1112 (Syrian), respectively. In conclusion, the study revealed a broad spectrum of resistance genes in Ab isolated from war-injured patients from Northern Africa and the Middle East, thereby broadening the scarcely available data on locally abundant clonal lineages and resistance mechanisms.
TiO2-Photocatalyzed Water Depollution, a Strong, yet Selective Depollution Method: New Evidence from the Solar Light Induced Degradation of Glucocorticoids in Freshwaters
Authors: Pretali Luca , Albini Angelo , Cantalupi Alice , Maraschi Federica , Nicolis Stefania , Sturini Michela
Published: March 2021

The photodegradation of the most prescribed glucocorticoids (GCs) was studied under relevant environmental conditions in the presence of suspended TiO2. The considered drugs included cortisone (CORT), hydrocortisone (HCORT), betamethasone (BETA), dexamethasone (DEXA), prednisone (PRED), prednisolone (PREDLO), and triamcinolone (TRIAM). The experiments were carried out at concentrations (50 µg L−1) close to the real ones in freshwater samples (tap and river) under simulated and natural sunlight, and their decomposition took place very efficiently under natural sunlight. The reactions were monitored by high-pressure liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). According to a pseudo-first-order decay, all drugs underwent degradation within 15 min, following different paths with respect to the direct photolysis. The observed kinetic constants, slightly lower in river than in tap water, varied from 0.29 to 0.61 min−1 with modest differences among GCs in the same matrix. Among main matrix macro-constituents, humic acids (HAs) were the most interfering species involved in GCs degradation. The photogenerated primary products were identified by HPLC-ESI-MS/MS, allowing to elucidate the general photochemical path of GCs. Finally, a comparison with literature data obtained using different advanced oxidation processes (AOPs) highlights the treatment efficiency with TiO2/solar light for removing such persistent aquatic contaminants.
Biodegradation of a Mixed Manure–Lignocellulosic System—A Possibility Study
Authors: Pečar Darja , Islamčević Razboršek Maša , Goršek Andreja
Published: March 2021

Animal waste is generated at an increased rate, and its disposal is attracting wide public attention. Anaerobic digestion is considered the most promising option for reducing this waste, and simultaneously, it produces renewable energy. Lignin contained in lignocellulosic biomass is hardly biodegradable, thus pre-treatment has to be considered prior to digestion. The possibility of biological pre-treatment of chicken manure with sawdust using Pleurotus ostreatus fungi was investigated in our study. This animal waste was used as a substrate for further biogas production. To provide a better nutrient balance, we added two different co-substrates, wheat straw and Miscanthus. Mixtures with different mass ratios of chicken manure with sawdust/ordinary wheat straw, as well as chicken manure with sawdust/pre-treated wheat straw were incubated for 30 d. The same experiments were performed with Miscanthus. During incubation, samples were taken at predetermined time intervals, and the concentration of acid-insoluble lignin was determined. Additionally, concentrations of glucose and xylose in the filtrate taken at the end of the Klasson procedure were determined in the initial samples and in the samples after 30 d of incubation. Despite our expectations, almost no lignin degradation was observed. Insignificant decreases in glucose and xylose concentrations after 30 d is attributed to fungi ingestion. Obtained results show that Pleurotus ostreatus, as a white-rot fungi with a unique enzymatic system and as generally preferred organisms for lignin degradation, is, therefore, not suitable for delignification of this particular animal waste.
Green Approaches to Carbon Nanostructure-Based Biomaterials
Authors: Adorinni Simone , Cringoli Maria C. , Perathoner Siglinda , Fornasiero Paolo , Marchesan Silvia
Published: March 2021

The family of carbon nanostructures comprises several members, such as fullerenes, nano-onions, nanodots, nanodiamonds, nanohorns, nanotubes, and graphene-based materials. Their unique electronic properties have attracted great interest for their highly innovative potential in nanomedicine. However, their hydrophobic nature often requires organic solvents for their dispersibility and processing. In this review, we describe the green approaches that have been developed to produce and functionalize carbon nanomaterials for biomedical applications, with a special focus on the very latest reports.
Assessment of Heavy Metal Contamination in Two Edible Fish Species and Water from North Patagonia Estuary
Authors: Fierro Pablo , Tapia Jaime , Bertrán Carlos , Acuña Cristina , Vargas-Chacoff Luis
Published: March 2021

Estuaries worldwide have been severely degraded and become reservoirs for many types of pollutants, such as heavy metals. This study investigated the levels of Cd, Cu, Mn, Ni, Pb, and Zn in water and whole fish. We sampled 40 juvenile silversides Odontesthes regia and 41 juvenile puye Galaxias maculatus from the Valdivia River estuary, adjacent to the urban area in southern South America (Chile). Samples were analyzed using a flame atomic absorption spectrophotometer. In water samples, metals except Zn were mostly below the detection limits and all metals were below the maximum levels established by local guidelines in this estuary. In whole fish samples, concentrations of Cu, Zn, Pb, Mn, and Cd were significantly higher in puyes than in silversides. Additionally, Zn, Pb, and Mn were correlated to body length and weight in puyes, whereas Cd was correlated to body length in silversides. The mean concentration of heavy metals in silverside and puyes were higher than those reported in the literature. In silversides, all heavy metal levels were below the limits permitted by current legislation (FAO), whereas in puyes Pb and Cd levels were above the recommended maximum level established by international guidelines, therefore putting the human population at risk.
Dental Implant Site Drilling and Induced Morphological Changes Correlated with Temperature in Pig’s Rib Used as the Human Jaw Model
Authors: Kirstein Karol , Horochowska Michalina , Jagiełło Jacek , Bubak Joanna , Chrószcz Aleksander , Kuropka Piotr , Dobrzyński Maciej , Poradowski Dominik , Michałek Marcin , Borawski Wojciech , Janeczek Maciej
Published: March 2021

The bone tissue destruction during drilling is still one of the crucial problems in implantology. In this study, the influence of drilling speed, coolant presence, and its temperature on bone tissue was tested using swine rib as a biological model of human jaws. The same method of drilling (with or without coolant) was used in all tested samples. The microscopic investigation estimated the size of the destruction zone and morphology of bone tissue surrounding the drilling canal. The achieved results were statistically elaborated. The study proved that the optimal drilling speed was ca. 1200 rpm, but the temperature of the used coolant had no significant influence on provoked bone destruction. Simultaneously, the drilling system without coolant compared to this with coolant has statistical importance on drilling results. Further in vivo studies will verify the obtained results.
Population Dynamics and Yeast Diversity in Early Winemaking Stages without Sulfites Revealed by Three Complementary Approaches
Authors: Windholtz Sara , Dutilh Lucie , Lucas Marine , Maupeu Julie , Vallet-Courbin Amélie , Farris Laura , Coulon Joana , Masneuf-Pomarède Isabelle
Published: March 2021

Nowadays, the use of sulfur dioxide (SO2) during the winemaking process is a controversial societal issue. In order to reduce its use, various alternatives are emerging, in particular bioprotection by adding yeasts, with different impacts on yeast microbiota in early winemaking stages. In this study, quantitative-PCR and metabarcoding high-throughput sequencing (HTS) were combined with MALDI-TOF-MS to monitor yeast population dynamic and diversity in the early stages of red winemaking process without sulfites and with bioprotection by Torulaspora delbrueckii and Metschnikowia pulcherrima addition. By using standard procedures for yeast protein extraction and a laboratory-specific database of wine yeasts, identification at species level of 95% of the isolates was successfully achieved by MALDI-TOF-MS, thus confirming that it is a promising method for wine yeast identification. The different approaches confirmed the implantation and the niche occupation of bioprotection leading to the decrease of fungal communities (HTS) and Hanseniaspora uvarum cultivable population (MALDI-TOF MS). Yeast and fungi diversity was impacted by stage of maceration and, to a lesser extent, by bioprotection and SO2, resulting in a modification of the nature and abundance of the operational taxonomic units (OTUs) diversity.
Effects of Different LED Light Recipes and NPK Fertilizers on Basil Cultivation for Automated and Integrated Horticulture Methods
Authors: Barbi Silvia , Barbieri Francesco , Bertacchini Alessandro , Barbieri Luisa , Montorsi Monia
Published: March 2021

This study aims to optimize the conditions for “Genovese” basil (Ocimum Basilicum) germination and growth in an indoor environment suitable for horticulture through a synergic effect of light and fertilizers addition. In fact, several studies determined that specific light conditions are capable of enhancing basil growth, but this effect is highly dependent on the environmental conditions. In this study, the effect of different light sources was determined employing a soil with a negligible amount of fertilizer, demonstrating substantial improvement when light-emitting diode (LED) lights (hyper red and deep blue in different combinations) were applied with respect to daylight (Plants height: +30%, Total fresh mass: +50%). Thereafter, a design of experiment approach has been implemented to calculate the specific combination of LED lights and fertilizer useful to optimize the basil growth. A controlled-release fertilizer based on nitrogen, phosphorus, and potassium (NPK) derived from agro-residues was compared with a soil enriched in macronutrients. The results demonstrate significant improvements for the growth parameters with the employment of the controlled-release NPK with respect to enriched soil combined with a ratio of hyper red and deep blue LED light equal to 1:3 (Total fresh mass: +100%, Leaves number: +20%).
A Minimal GBT Model for Distortional-Twist Elastic Analysis of Box-Girder Bridges
Authors: Pancella Francesca , Luongo Angelo
Published: March 2021

A simple and efficient method is proposed for the analysis of twist of rectangular box-girder bridges, which undergo distortion of the cross section. The model is developed in the framework of the Generalized Beam Theory and oriented towards semi-analytical solutions. Accordingly, only two modes are accounted for: (i) the torsional mode, in which the box-girder behaves as a Vlasov beam under nonuniform torsion, and, (ii) a distortional mode, in which the cross section behaves as a planar frame experiencing skew-symmetric displacements. By following a variational approach, two coupled, fourth-order differential equations in the modulating amplitudes are obtained. The order of magnitude of the different terms is analyzed, and further reduced models are proposed. A sample system, taken from the literature, is considered, for which generalized displacement and stress fields are evaluated. Both a Fourier solution for the coupled problem and a closed-form solution for the uncoupled problem are carried out, and the results are compared. Finally, the model is validated against finite element analyses.
Biologics—An Open Access Journal for Biological Drugs
Authors: Capasso Raffaele
Published: March 2021

Biological drugs have been attracting interest from the scientific community in recent years [...]
Acute Effects of Surgical and FFP2 Face Masks on Physiological Responses and Strength Performance in Persons with Sarcopenia
Authors: Ramos-Campo Domingo Jesús , Pérez-Piñero Silvia , Muñoz-Carrillo Juan Carlos , López-Román Francisco Javier , García-Sánchez Esther , Ávila-Gandía Vicente
Published: March 2021

Due to COVID-19, wearing a face mask to reduce virus transmission is currently mandatory in some countries when participants practice exercise in sports centers. Therefore, the aim of the present study was to analyze the effect of wearing a surgical or FFP2 mask during a resistance training session. Fourteen people with sarcopenia (age: 59.40 ± 5.46 years; weight: 68.78 ± 8.31 kg; height: 163.84 ± 9.08 cm) that participated in the study performed three training sessions in a randomized order: 4 sets of 10 repetitions of a half-squat at 60% of the one-repetition maximum and 90 s of rest between set and were either (a) without a mask (NM), (b) wearing a surgical face mask (SM), and (c) wearing a FFP2 face mask (FFP2). We found that wearing face masks had no effect on strength performance (session mean propulsive velocity (m/s): WM: 0.396 ± 0.042; SM: 0.387 ± 0.037; and FFP2: 0.391 ± 0.042 (p = 0.918)). Additionally, no impact of wearing a mask was found on heart rate, heart rate variability, blood lactate concentration (WM: 4.17 ± 1.89; SM: 4.49 ± 2.07; and FFP2: 5.28 ± 2.45 mmol/L (p = 0.447)), or rating of perceived exertion. Wearing a surgical or FFP2 face mask during a resistance training session resulted in similar strength performance and physiological responses than the same exercise without a mask in persons with sarcopenia.