bluuebunny/crossref_metadata_2025 huggingface dataset:
An Antibody of the Secreted Isoform of Disintegrin and Metalloprotease 9 (sADAM9) Inhibits Epithelial–Mesenchymal Transition and Migration of Prostate Cancer Cell Lines
Authors: Jotatsu Yura , Sung Shain-Ying , Wu Ming-Heng , Takeda Shunya , Hirata Yuto , Maeda Koki , Fang Shiuh-Bin , Chen Kuan-Chou , Shigemura Katsumi
Published: June 2024

Prostate cancer (PC) is the most common cancer diagnosed in men worldwide. Currently, castration-resistant prostate cancer (CRPC), which is resistant to androgen deprivation therapy, has a poor prognosis and is a therapeutic problem. We investigated the antitumor effects on PC of an antibody neutralizing secreted disintegrin and metalloproteinase domain-containing protein 9 (sADAM9), which is a blood-soluble form. We performed proliferation assays, wound healing assays, invasion assays, Western blot (WB), and an in vivo study in which a sADAM9 neutralizing antibody was administered intratumorally to PC-bearing mice. In invasion assays, the sADAM9 neutralizing antibody significantly inhibited invasion in all cell lines (TRAMP-C2: p = 0.00776, LNCaP: p = 0.000914, PC-3: p = 0.0327, and DU145: p = 0.0254). We examined epithelial–mesenchymal transition (EMT) markers, one of the metastatic mechanisms, in WB and showed downregulation of Slug in TRAMP-C2, LNCaP, and DU145 and upregulation of E-cadherin in TRAMP-C2 and PC-3 by sADAM9 neutralization. In mouse experiments, the sADAM9 neutralizing antibody significantly suppressed tumor growth compared to controls (1.68-fold in TRAMP-C2, 1.89-fold in LNCaP, and 2.67-fold in PC-3). These results suggested that the sADAM9 neutralizing antibody inhibits invasion, migration, and tumor growth in PC. Previous studies examined the anti-tumor effect of knockdown of total ADAM9 or sADAM9, but this study used the new technology of neutralizing antibodies for sADAM9. This may be novel because there was no animal study using a neutralizing antibody for sADAM9 to see the relationship between ADAM9 expression and prostate cancer.
Local E-rhBMP-2/β-TCP Application Rescues Osteocyte Dendritic Integrity and Reduces Microstructural Damage in Alveolar Bone Post-Extraction in MRONJ-like Mouse Model
Authors: Dang Anh Tuan , Ono Mitsuaki , Wang Ziyi , Tosa Ikue , Hara Emilio Satoshi , Mikai Akihiro , Kitagawa Wakana , Yonezawa Tomoko , Kuboki Takuo , Oohashi Toshitaka
Published: June 2024

The pathology of medication-related osteonecrosis of the jaw (MRONJ), often associated with antiresorptive therapy, is still not fully understood. Osteocyte networks are known to play a critical role in maintaining bone homeostasis and repair, but the exact condition of these networks in MRONJ is unknown. On the other hand, the local application of E-coli-derived Recombinant Human Bone Morphogenetic Protein 2/β-Tricalcium phosphate (E-rhBMP-2/β-TCP) has been shown to promote bone regeneration and mitigate osteonecrosis in MRONJ-like mouse models, indicating its potential therapeutic application for the treatment of MRONJ. However, the detailed effect of BMP-2 treatment on restoring bone integrity, including its osteocyte network, in an MRONJ condition remains unclear. Therefore, in the present study, by applying a scanning electron microscope (SEM) analysis and a 3D osteocyte network reconstruction workflow on the alveolar bone surrounding the tooth extraction socket of an MRONJ-like mouse model, we examined the effectiveness of BMP-2/β-TCP therapy on the alleviation of MRONJ-related bone necrosis with a particular focus on the osteocyte network and alveolar bone microstructure (microcrack accumulation). The 3D osteocyte dendritic analysis showed a significant decrease in osteocyte dendritic parameters along with a delay in bone remodeling in the MRONJ group compared to the healthy counterpart. The SEM analysis also revealed a notable increase in the number of microcracks in the alveolar bone surface in the MRONJ group compared to the healthy group. In contrast, all of those parameters were restored in the E-rhBMP-2/β-TCP-treated group to levels that were almost similar to those in the healthy group. In summary, our study reveals that MRONJ induces osteocyte network degradation and microcrack accumulation, while application of E-rhBMP-2/β-TCP can restore a compromised osteocyte network and abrogate microcrack accumulation in MRONJ.
The Modulation of Respiratory Epithelial Cell Differentiation by the Thickness of an Electrospun Poly-ε-Carprolactone Mesh Mimicking the Basement Membrane
Authors: Choi Seon Young , Kim Hyun Joo , Hwang Soyoung , Park Jangho , Park Jungkyu , Lee Jin Woo , Son Kuk Hui
Published: June 2024

The topology of the basement membrane (BM) affects cell physiology and pathology, and BM thickening is associated with various chronic lung diseases. In addition, the topology of commercially available poly (ethylene terephthalate) (PET) membranes, which are used in preclinical in vitro models, differs from that of the human BM, which has a fibrous and elastic structure. In this study, we verified the effect of BM thickness on the differentiation of normal human bronchial epithelial (NHBE) cells. To evaluate whether the thickness of poly-ε-carprolactone (PCL) mesh affects the differentiation of NHBE cells, cells were grown on thin- (6-layer) and thick-layer (80-layer) meshes consisting of electrospun PCL nanofibers using an air–liquid interface (ALI) cell culture system. It was found that the NHBE cells formed a normal pseudostratified epithelium composed of ciliated, goblet, and basal cells on the thin-layer PCL mesh; however, goblet cell hyperplasia was observed on the thick-layer PCL mesh. Differentiated NHBE cells cultured on the thick-layer PCL mesh also demonstrated increased epithelial–mesenchymal transition (EMT) compared to those cultured on the thin-layer PCL mesh. In addition, expression of Sox9, nuclear factor (NF)-κB, and oxidative stress-related markers, which are also associated with goblet cell hyperplasia, was increased in the differentiated NHBE cells cultured on the thick-layer PCL mesh. Thus, the use of thick electrospun PCL mesh led to NHBE cells differentiating into hyperplastic goblet cells via EMT and the oxidative stress-related signaling pathway. Therefore, the topology of the BM, for example, thickness, may affect the differentiation direction of human bronchial epithelial cells.
Cannabidiol Protects against the Reinstatement of Oxycodone-Induced Conditioned Place Preference in Adolescent Male but Not Female Rats: The Role of MOR and CB1R
Authors: Socha Justyna , Grochecki Pawel , Marszalek-Grabska Marta , Skrok Aleksandra , Smaga Irena , Slowik Tymoteusz , Prazmo Wojciech , Kotlinski Robert , Filip Malgorzata , Kotlinska Jolanta H.
Published: June 2024

Cannabidiol (CBD), a phytocannabinoid, appeared to satisfy several criteria for a safe approach to preventing drug-taking behavior, including opioids. However, most successful preclinical and clinical results come from studies in adult males. We examined whether systemic injections of CBD (10 mg/kg, i.p.) during extinction of oxycodone (OXY, 3 mg/kg, i.p.) induced conditioned place preference (CPP) could attenuate the reinstatement of CPP brought about by OXY (1.5 mg/kg, i.p.) priming in adolescent rats of both sexes, and whether this effect is sex dependent. Accordingly, a priming dose of OXY produced reinstatement of the previously extinguished CPP in males and females. In both sexes, this effect was linked to locomotor sensitization that was blunted by CBD pretreatments. However, CBD was able to prevent the reinstatement of OXY-induced CPP only in adolescent males and this outcome was associated with an increased cannabinoid 1 receptor (CB1R) and a decreased mu opioid receptor (MOR) expression in the prefrontal cortex (PFC). The reinstatement of CCP in females was associated with a decreased MOR expression, but no changes were detected in CB1R in the hippocampus (HIP). Moreover, CBD administration during extinction significantly potentialized the reduced MOR expression in the PFC of males and showed a tendency to potentiate the reduced MOR in the HIP of females. Additionally, CBD reversed OXY-induced deficits of recognition memory only in males. These results suggest that CBD could reduce reinstatement to OXY seeking after a period of abstinence in adolescent male but not female rats. However, more investigation is required.
Assessment of Total Antioxidant Capacity, 8-Hydroxy-2′-deoxy-guanosine, the Genetic Landscape, and Their Associations in BCR::ABL-1-Negative Chronic and Blast Phase Myeloproliferative Neoplasms
Authors: Găman Mihnea-Alexandru , Mambet Cristina , Neagu Ana Iulia , Bleotu Coralia , Gurban Petruta , Necula Laura , Botezatu Anca , Ataman Marius , Diaconu Camelia Cristina , Ionescu Bogdan Octavian , Ghiaur Alexandra Elena , Tatic Aurelia , Coriu Daniel , Găman Amelia Maria , Diaconu Carmen Cristina
Published: June 2024

Myeloproliferative neoplasms (MPNs), namely, polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF), are clonal stem cell disorders defined by an excessive production of functionally mature and terminally differentiated myeloid cells. MPNs can transform into secondary acute myeloid leukemia (sAML/blast phase MPN) and are linked to alterations in the redox balance, i.e., elevated concentrations of reactive oxygen species and markers of oxidative stress (OS), and changes in antioxidant systems. We evaluated OS in 117 chronic phase MPNs and 21 sAML cases versus controls by measuring total antioxidant capacity (TAC) and 8-hydroxy-2′-deoxy-guanosine (8-OHdG) concentrations. TAC was higher in MPNs than controls (p = 0.03), particularly in ET (p = 0.04) and PMF (p = 0.01). MPL W515L-positive MPNs had higher TAC than controls (p = 0.002) and triple-negative MPNs (p = 0.01). PMF patients who had treatment expressed lower TAC than therapy-free subjects (p = 0.03). 8-OHdG concentrations were similar between controls and MPNs, controls and sAML, and MPNs and sAML. We noted associations between TAC and MPNs (OR = 1.82; p = 0.05), i.e., ET (OR = 2.36; p = 0.03) and PMF (OR = 2.11; p = 0.03), but not sAML. 8-OHdG concentrations were not associated with MPNs (OR = 1.73; p = 0.62) or sAML (OR = 1.89; p = 0.49). In conclusion, we detected redox imbalances in MPNs based on disease subtype, driver mutations, and treatment history.
A Narrative Review of Intestinal Microbiota’s Impact on Migraine with Psychopathologies
Authors: Francavilla Miriam , Facchetti Sara , Demartini Chiara , Zanaboni Anna Maria , Amoroso Chiara , Bottiroli Sara , Tassorelli Cristina , Greco Rosaria
Published: June 2024

Migraine is a common and debilitating neurological disorder characterized by the recurrent attack of pulsating headaches typically localized on one side of the head associated with other disabling symptoms, such as nausea, increased sensitivity to light, sound and smell and mood changes. Various clinical factors, including the excessive use of migraine medication, inadequate acute treatment and stressful events, can contribute to the worsening of the condition, which may evolve to chronic migraine, that is, a headache present on >15 days/month for at least 3 months. Chronic migraine is frequently associated with various comorbidities, including anxiety and mood disorders, particularly depression, which complicate the prognosis, response to treatment and overall clinical outcomes. Emerging research indicates a connection between alterations in the composition of the gut microbiota and mental health conditions, particularly anxiety and depression, which are considered disorders of the gut–brain axis. This underscores the potential of modulating the gut microbiota as a new avenue for managing these conditions. In this context, it is interesting to investigate whether migraine, particularly in its chronic form, exhibits a dysbiosis profile similar to that observed in individuals with anxiety and depression. This could pave the way for interventions aimed at modulating the gut microbiota for treating difficult-to-manage migraines.
Peroxiporins in Triple-Negative Breast Cancer: Biomarker Potential and Therapeutic Perspectives
Authors: Bijelić Anita , Silovski Tajana , Mlinarić Monika , Čipak Gašparović Ana
Published: June 2024

Triple-negative breast cancer (TNBC) remains one of the most challenging subtypes since it is initially characterized by the absence of specific biomarkers and corresponding targeted therapies. Advances in methodology, translational informatics, genomics, and proteomics have significantly contributed to the identification of therapeutic targets. The development of innovative treatments, such as antibody–drug conjugates and immune checkpoint inhibitors, alongside chemotherapy, has now become the standard of care. However, the quest for biomarkers defining therapy outcomes is still ongoing. Peroxiporins, which comprise a subgroup of aquaporins, which are membrane pores facilitating the transport of water, glycerol, and hydrogen peroxide, have emerged as potential biomarkers for therapy response. Research on peroxiporins reveals their involvement beyond traditional channeling activities, which is also reflected in their cellular localization and roles in cellular signaling pathways. This research on peroxiporins provides fresh insights into the mechanisms of therapy resistance in tumors, offering potential avenues for predicting treatment outcomes and tailoring successful TNBC therapies.
Aptamer and Electrochemical Aptasensor towards Selenate Ions (SeO42−)
Authors: Szymczyk Anna , Popiołek Martyna , Baran Dominika , Olszewski Marcin , Ziółkowski Robert , Malinowska Elżbieta
Published: June 2024

Selenium is an essential inorganic compound in human and animal nutrition, involved in the proper functioning of the body. As a micronutrient, it actively contributes to the regulation of various metabolic activities, i.e., thyroid hormone, and protection against oxidative stress. However, Se exhibits a narrow concentration window between having a positive effect and exerting a toxic effect. In higher doses, it negatively affects living organisms and causes DNA damage through the formation of free radicals. Increased reactivity of Se anions can also disrupt the integrity and function of DNA-repairing proteins. As the permissible concentration of Se in drinking water is 10 µg/L, it is vital to develop sensitive and robust methods of Se detection in aqueous samples. In this study, for the first time, we proposed a selective aptamer for selenate ion detection, chosen following the SELEX process, and its application in the construction of an electrochemical aptasensor towards SeO42− ions. Measurement conditions such as the used redox marker and pH value of the measurement solution were chosen. The proposed aptasensor is characterized by good selectivity and an LOD of 1 nM. Conditions for biosensor regeneration and storage were also investigated in this research.
Involvement of Reactive Oxygen Species in Prostate Cancer and Its Disparity in African Descendants
Authors: Liou Geou-Yarh , C’lay-Pettis Reauxqkwuanzyiia , Kavuri Sravankumar
Published: June 2024

Reactive oxygen species (ROS) participate in almost all disorders, including cancer. Many factors, including aging, a high-fat diet, a stressful lifestyle, smoking, infection, genetic mutations, etc., lead to elevated levels of ROS. Prostate cancer, the most prevalent type of cancer in senior American men and the second leading cause of cancer mortality in American men, results from chronic oxidative stress. The doubled incident rate as well as the doubled mortality numbers of prostate cancer have persisted in African Americans in comparison with Caucasian Americans and other racial groups, indicating a prostate cancer disparity in African American men. In this review, we mainly focus on the latest findings on ROS in prostate cancer development and progression within the last five years to update our understanding in this area, as several comprehensive literature reviews addressing oxidative stress and/or inflammation in prostate cancer before 2020 are available. In addition to other known factors such as socioeconomic disadvantage, cultural mistrust of the health care system, etc. that are long-existing in the African American group, we also summarize the latest evidence that demonstrated high systemic oxidative stress and inflammation in African Americans for their potential contribution to the racial prostate cancer disparity in this population.
Investigating the Anticancer Properties of Novel Functionalized Platinum(II)–Terpyridine Complexes
Authors: Panebianco Roberta , Viale Maurizio , Giglio Valentina , Vecchio Graziella
Published: June 2024

Novel platinum(II) complexes of 4′-substituted terpyridine ligands were synthesized and characterized. Each complex had a different biomolecule (amine, glucose, biotin and hyaluronic acid) as a targeting motif, potentially improving therapeutic outcomes. We demonstrated that complexes can self-assemble in water into about 150 nm nanoparticles. Moreover, the complexes were assayed in vitro toward a panel of human cancer cell lines (ovarian adenocarcinoma A2780, lung cancer A549, breast adenocarcinoma MDA-MB-231, neuroblastoma SHSY5Y) to explore the impact of the pendant moiety on the terpyridine toxicity. The platinum complex of terpyridine amine derivative, [Pt(TpyNH2)Cl]Cl, showed the best antiproliferative effect, which was higher than cisplatin and [Pt(Tpy)Cl]Cl. Selective in vitro antiproliferative activity was achieved in A549 cancer cells with the Pt–HAtpy complex. These findings underline the potential of these novel platinum(II) complexes in cancer therapy and highlight the importance of tailored molecular design for achieving enhanced therapeutic effects.
Bio-Inspired Helicoidal Composite Structure Featuring Graded Variable Ply Pitch under Transverse Tensile Loading
Authors: Malekinejad Hossein , Carbas Ricardo J. C. , Akhavan-Safar Alireza , Marques Eduardo A. S. , Ferreira Maria , da Silva Lucas F. M.
Published: June 2024

Biostructures found in nature exhibit remarkable strength, toughness, and damage resistance, achieved over millions of years. Observing nature closely might help develop laminates that resemble natural structures more closely, potentially improving strength and mimicking natural principles. Bio-inspired Carbon Fiber-Reinforced Polymers (CFRP) investigated thus far exhibit consistent pitch angles between layers, whereas natural structures display gradual variations in pitch angle rather than consistency. Therefore, this study explores helicoidal CFRP laminates, focusing on the Non-Linear Rotation Angle (NLRA) or gradual variation to enhance composite material performance. In addition, it compares the strength and failure mechanisms of the gradual configuration with conventional helicoidal and unidirectional (UD) laminates, serving as references while conducting transverse tensile tests (out-of-plane tensile). The findings highlight the potential of conventional and gradual helicoidal structures in reinforcing CFRP laminates, increasing the failure load compared to unidirectional CFRP laminate by about 5% and 17%, respectively. In addition, utilizing bio-inspired configurations has shown promising improvements in toughness compared to traditional unidirectional laminates, as evidenced by the increased displacement at failure. The numerical and experimental analyses revealed a shift in crack path when utilizing the bio-inspired helicoidal stacking sequence. Validated by experimental data, this alteration demonstrates longer and more intricate crack propagation, ultimately leading to increased transverse strength.
Keratinous Natural Fibres as Sustainable Flame Retardants and Reinforcements in Polymer Composites
Authors: Mishra Avishek , Kim Nam Kyeun , Bhattacharyya Debes
Published: June 2024

Natural fibres have been used as fibre reinforcements in composites as they offer eco-friendly and economic advantages, but their susceptibility to deterioration when exposed to heat and flames has limited their practical application in fibre-reinforced polymeric composites. Fire-reaction properties have been explored in reasonable detail for plant fibres, but a gap exists in the understanding of animal fibre-reinforced composites. Understanding the thermal and fire reactions of these keratin-rich animal fibres is crucial for material selection and advancing composite product development. The current paper critically discusses the existing research landscape and suggests future research directions. The use of keratinous fibres in composites can definitely improve their thermal stability and fire performance, but it also appears to adversely affect the composite’s mechanical performance. The main part of this paper focuses on the flame-retardant treatment of keratinous fibres and polymer composites, and their behaviour under fire conditions. The final part of this paper includes a brief look at the environmental impact of the treatment methods; the overall processing of keratinous fibre-reinforced composites is also presented to gain further insight.
Are Surface Electromyography Parameters Indicative of Post-Activation Potentiation/Post-Activation Performance Enhancement, in Terms of Twitch Potentiation and Voluntary Performance? A Systematic Review
Authors: Gallardo Philip , Giakas Giannis , Sakkas Giorgos K. , Tsaklis Panagiotis V.
Published: June 2024

The aim was to identify if surface electromyography (sEMG) parameters are indicative of post-activation potentiation (PAP)/post-activation performance enhancement (PAPE), in terms of twitch potentiation and voluntary performance. Three databases were used in April 2024, with the following inclusion criteria: (a) original research, assessed in healthy human adults, and (b) sEMG parameters were measured. The exclusion criteria were (a) studies with no PAP/PAPE protocol and (b) non-randomized control trials. The following data were extracted: study characteristics/demographics, PAP/PAPE protocols, sEMG parameters, twitch/performance outcomes, and study findings. A modified physiotherapy evidence database (PEDro) scale was used for quality assessment. Fifteen randomized controlled trials (RCTs), with a total of 199 subjects, were included. The M-wave amplitude (combined with a twitch torque outcome) was shown to generally be indicative of PAP. The sEMG amplitudes (in some muscles) were found to be indicative of PAPE during ballistic movements, while a small decrease in the MdF (in certain muscles) was shown to reflect PAPE. Changes in the Hmax/Mmax ratio were found to contribute (temporally) to PAP, while the H-reflex amplitude was shown to be neither indicative of PAP nor PAPE. This review provides preliminary findings suggesting that certain sEMG parameters could be indicative of PAP/PAPE. However, due to limited studies, future research is warranted.
Research on Evaluation of the Carbon Dioxide Sequestration Potential in Saline Aquifers in the Qiongdongnan–Yinggehai Basin
Authors: Tian Yukun , Du Zhili , Zhang Lin , Zhang Lizhong , Xu Guoqiang , Chen Jiaojiao
Published: June 2024

This paper evaluates the carbon dioxide sequestration potential in the saline aquifers of the South Qiongdongnan–Yinggehai Basin. By using a hierarchical evaluation method, the assessment is divided into five stages: the basin level, the zone level, the target level, the site level, and the injection level. The study primarily focuses on evaluating the sequestration potential of and identifying favorable zones of saline aquifers at the basin and zone levels. The optimized volumetric method is adopted, based on the integration of multi-source data such as regional geological maps, seismic data, core porosity, and permeability. The results show that the estimated potential of the Yinggehai Basin is 60.6 billion tons at the basin level and 54.6 billion tons at the zone level. Additionally, the estimated potential of the South Qiongdongnan Basin is 261.5 billion tons at the basin level and 234.8 billion tons at the zone level. The suitability evaluation indicates that the Yinggehai Basin is moderately suitable overall, the northern depression of the South Qiongdongnan Basin is suitable, the central uplift is moderately suitable, and the central depression is not suitable. This study provides a scientific foundation for carbon dioxide sequestration in marine basins and introduces novel ideas and methods for future similar research. This is highly significant for subsequent engineering applications and decision-making processes.
Failure Analysis of a Suspended Inter-Array Power Cable between Two Spar-Type Floating Wind Turbines: Evaluating the Influence of Buoy Element Failure on the Cable
Authors: Liu Dan , Janocha Marek Jan , Ahmad Izwan Bin , Ong Muk Chen
Published: June 2024

The suspended configuration of inter-array power cables between floating offshore wind turbines necessitates using various ancillary equipment, such as buoy elements and bend stiffeners, to maintain the desired cable geometry. The failure analysis is an important step in the design of an inter-array dynamic power cable layout. This study investigates the impact of buoy element failures on the structural integrity and fatigue life of inter-array power cable configurations in offshore environments, focusing on four environmental conditions representative of the North Sea. Utilizing numerical simulations and fatigue analysis in OrcaFlex, static and dynamic analyses are conducted to assess maximum tension, minimum bend radius (MBR), and fatigue life under single and two failure scenarios of buoy elements. The results indicate that single buoy failures significantly increase maximum tension at hang-off points. At the same time, MBR is found to be the smallest at the failure position, aiding in failure point identification. In addition, for the two buoy element failure scenarios, the maximum tension increase poses risks to structural integrity, while MBR and fatigue life have high sensitivity to the applied environmental conditions.
Inbred Mouse Models in Cryptococcus neoformans Research
Authors: Ding Minna , Nielsen Kirsten
Published: June 2024

Animal models are frequently used as surrogates to understand human disease. In the fungal pathogen Cryptococcus species complex, several variations of a mouse model of disease were developed that recapitulate different aspects of human disease. These mouse models have been implemented using various inbred and outbred mouse backgrounds, many of which have genetic differences that can influence host response and disease outcome. In this review, we will discuss the most commonly used inbred mouse backgrounds in C. neoformans infection models.
Public Evaluations of Misinformation and Motives for Sharing It
Authors: Osman Magda
Published: June 2024

Concerns around the definition of misinformation hamper ways of addressing purported problems associated with it, along with the fact that public understanding of the concept is often ignored. To this end, the present pilot survey study examines three broad issues, as follows: (1) contexts where the concept most applies to (i.e., face-to-face interactions, social media, news media, or all three contexts), (2) criteria people use to identify misinformation, and (3) motivations for sharing it. A total of 1897 participants (approximately 300 per country) from six different countries (Chile, Germany, Greece, Mexico, the UK, the USA) were asked questions on all three, along with an option to provide free text responses for two of them. The quantitative and qualitative findings reveal a nuanced understanding of the concept, with the common defining characteristics being claims presented as fact when they are opinion (71%), claims challenged by experts (66%), and claims that are unqualified by evidence (64%). Moreover, of the 28% (n = 538) of participants providing free text responses further qualifying criteria for misinformation, 31% of them mentioned critical details from communication (e.g., concealing relevant details or lacking evidence to support claims), and 41% mentioned additions in communication that reveal distortions (e.g., sensationalist language, exaggerating claims). Rather than being exclusive to social media, misinformation was seen by the full sample (n = 1897) as present in all communication contexts (59%) and is shared for amusement (50%) or inadvertently (56%).
Artificial Intelligence Used for Diagnosis in Facial Deformities: A Systematic Review
Authors: Ravelo Victor , Acero Julio , Fuentes-Zambrano Jorge , García Guevara Henry , Olate Sergio
Published: June 2024

AI is included in a lot of different systems. In facial surgery, there are some AI-based software programs oriented to diagnosis in facial surgery. This study aims to evaluate the capacity and training of models for diagnosis of dentofacial deformities in class II and class III patients using artificial intelligence and the potential use for indicating orthognathic surgery. The search strategy is from 1943 to April 2024 in PubMed, Embase, Scopus, Lilacs, and Web of Science. Studies that used imaging to assess anatomical structures, airway volume, and craniofacial positions using the AI algorithm in the human population were included. The methodological quality of the studies was assessed using the Effective Public Health Practice Project instrument. The systematic search identified 697 articles. Eight studies were obtained for descriptive analysis after exclusion according to our inclusion and exclusion criteria. All studies were retrospective in design. A total of 5552 subjects with an age range between 14.7 and 56 years were obtained; 2474 (44.56%) subjects were male, and 3078 (55.43%) were female. Six studies were analyzed using 2D imaging and obtained highly accurate results in diagnosing skeletal features and determining the need for orthognathic surgery, and two studies used 3D imaging for measurement and diagnosis. Limitations of the studies such as age, diagnosis in facial deformity, and the included variables were observed. Concerning the overall analysis bias, six studies were at moderate risk due to weak study designs, while two were at high risk of bias. We can conclude that, with the few articles included, using AI-based software allows for some craniometric recognition and measurements to determine the diagnosis of facial deformities using mainly 2D analysis. However, it is necessary to perform studies based on three-dimensional images, increase the sample size, and train models in different populations to ensure accuracy of AI applications in this field. After that, the models can be trained for dentofacial diagnosis
The Impact of Food Enrichment on the Behavior of Cownose Ray (Rhinoptera bonasus) Kept under Human Care
Authors: Barros Isabella Brosens , de Azevedo Cristiano Schetini , Cipreste Cynthia Fernandes , Reisfeld Laura Chrispim , Suzana Thais , Capriolli Rafael Gutierrez , Pizzutto Cristiane Schilbach
Published: June 2024

The cownose ray (Rhinoptera bonasus) faces vulnerability primarily due to unregulated fishing, resource overexploitation, and habitat degradation. Consequently, individuals maintained under human care play a pivotal role in species conservation, particularly when their welfare is prioritized. Achieving optimal welfare in aquarium settings relies heavily on effective management practices, notably environmental enrichment. However, research on the efficacy of such techniques for cownose rays remains limited. Thus, this study sought to evaluate the impact of various food enrichment items on the behavior of four individuals at the São Paulo Aquarium in Brazil. The project encompassed three phases: baseline, enrichment, and post-enrichment. Enrichment items, designed to mimic the species’ natural foraging behavior, included an ice block containing food, food hidden in vegetables fixed to structures at the bottom of the tank, a tray with substrate and food, and a perforated plastic container with food inside. Behavioral observations utilized focal sampling with instantaneous recording every minute. Results showed increased foraging activity in the post-enrichment phase, whereas swimming increased and following behaviors decreased during the enrichment phase. Additionally, foraging behaviors predominantly occurred near the aquarium bottom. Overall, findings suggest that enrichment items effectively stimulated natural behaviors in cownose rays and were very attractive to the fish, advocating for their integration into species management protocols to enhance welfare.
Sixty Years of Tinbergen’s Four Questions and Their Continued Relevance to Applied Behaviour and Welfare Research in Zoo Animals: A Commentary
Authors: Kelly Robert , Rose Paul
Published: June 2024

Understanding animal behaviour can feel like deciphering a foreign language. In 1963, pioneering ethologist Nikolaas Tinbergen offered a key: four fundamental questions to dissect behaviour’s complexities and reduce interpretive bias. These “Four Questions” fall into two categories: Proximate (how?) and Ultimate (why?). The Proximate questions ask how the behaviour is triggered (Causation) and develops over time (Ontogeny). The Ultimate questions delve into its evolutionary history (Phylogeny) and purpose (Function). Traditionally used in behavioural ecology, Tinbergen’s framework finds new relevance in fields like sentience, welfare, conservation, and animal management. This paper illustrates how further integration of these Questions into applied research can improve outcomes. For example, captive animals can receive enrichment seemingly “unnatural” in origin and form. Does such enrichment trigger species-typical behaviours, fulfilling the same adaptive function as natural stimuli would? Understanding a species’ natural behaviour patterns and how the performance of such activities promotes positive welfare states is key to biologically relevant population management. Tinbergen’s Four Questions can help scientists to decipher the relevance of natural behaviour, and how a species’ responses to their environment indicate what individuals need and want at a specific time or place. By applying the Four Questions, we can answer this question and, in turn, refine husbandry practices and conserve behavioural diversity in managed populations. Sixty years after their conception, Tinbergen’s Four Questions remain a powerful tool for behavioural research. By embracing different biological disciplines within a unified framework, applied animal zoo science will continue to advance and provide credible evidence-based outputs.
Understanding Indigenous Knowledge in Contemporary Consumption: A Framework for Indigenous Market Research Knowledge, Philosophy, and Practice from Aotearoa
Authors: Love Tyron Rakeiora , Hall C. Michael
Published: June 2024

Despite increased attention being given to Indigenous rights, decolonization, and reconciliation in a broader business setting, the engagement of business, marketing, and consumer studies with Indigenous cultures and peoples is negligible. Although Indigenous and First Nations peoples have a significant position in the social sciences, there is no specific body of marketing or consumer knowledge that is dedicated to Indigenous knowledge and practices, even though there is a growing interest in more inclusive and transformative marketing. This paper reports on current research on Indigenous worldviews and marketing, with a continuum of Indigenous research being presented which is particularly informed by Māori experiences in Aotearoa New Zealand. Several appropriate research methods for advancing Indigenous knowledge are presented. The paper concludes by noting the potential contributions that Indigenous knowledge may provide and some of the challenges faced.
Methodological Rigor in Laboratory Education Research
Authors: Agustian Hendra Y.
Published: June 2024

Despite the growing number of published studies on student learning in the laboratory, there is a critical need to improve methodological rigor. Resonating with discussions on research methods, this paper outlines the importance of theory-informed research questions, the minimization of researcher and participant biases, and the use of triangulation and iteration in data collection to establish rigor. An illustrative case is presented within the context of a large interdisciplinary research project aimed at improving laboratory learning at the university level. The project incorporates two research avenues: one focusing on student and faculty perspectives, and the other on a comprehensive assessment of multidimensional learning in the laboratory. The project employs a mixed methods paradigm and is grounded in a conceptual framework that conceptualizes laboratory work as epistemic practice, requiring a holistic analysis of student learning. The article concludes by discussing the results and implications of the project’s findings, which are synthesized to highlight aspects of establishing methodological rigor. The overarching goal is to develop a comprehensive assessment instrument that captures the complexity and richness of the laboratory learning environment. The findings from this research are expected to contribute to the advancement of laboratory education research by providing a model for methodological rigor that can be applied across various scientific and interdisciplinary contexts.
Understanding Outdoor Cold Stress and Thermal Perception of the Elderly in Severely Cold Climates: A Case Study in Harbin
Authors: He Xiaoyun , Shao Long , Tang Yuexing , Hao Liangbo
Published: June 2024

This study collected data through microclimate monitoring, surface temperature measurements, and questionnaire surveys, and used indicators, such as the universal thermal climate index (UTCI), surface temperature (Ts), and wind chill temperature (tWC), to determine the thermal comfort threshold of the elderly in severely cold climates and evaluate their cold stress. The results indicated that (1) the neutral UTCI (NUTCI) for elderly individuals in winter was 13.3 °C, and the NUTCI range was from 1.4 to 25.2 °C; (2) the intensity of elderly individuals’ physical activity affected the magnitude of risk of whole-body cooling, with duration-limited exposures corresponding to 0.5, 3.3, and over 8 h for light, moderate, and vigorous activity levels, respectively; (3) the tWC in all four spaces was below −10 °C, potentially inducing discomfort or even frostbite in the elderly; (4) for a 10 s touch, the maximum Ts (−17.2 °C) of stone was lower than the numbness threshold (−15.0 °C), while that (−15.1 °C) of steel materials remained below the frostbite threshold (−13 °C), posing risks for the elderly during physical activity. This study’s results will provide valuable insights and theoretical references for the landscape design of urban park activity spaces for elderly individuals in cold climate regions.
Exploring Public Support for Bolivia’s Protected Areas: A Contingent Valuation Approach
Authors: Rakela Stefanie , Vilela Thais , Espinoza Sophia , Malky Harb Alfonso , Mendizábal Vergara Carla
Published: June 2024

This study investigates Bolivians’ willingness to pay for an annual passport facilitating visits to protected areas (PAs) in Bolivia, aiming to address challenges in PA financing and management amidst escalating deforestation. Employing the contingent valuation method, a representative sample of citizens aged 18 or older from major cities across Bolivia participated in a telephone interviewing questionnaire. The findings reveal limited public awareness and recognition of PAs, alongside significant interest in visiting these areas for tourism purposes or simply donating to the system to reduce its current financial gaps. Concerns regarding trust and transparency in fund management are highlighted, with demographic factors and perceptions influencing willingness to pay estimated at approximately USD 35 for a one-year passport. This study underscores the necessity for targeted interventions to enhance public awareness, improve infrastructure, and ensure transparency in fund management, thereby promoting sustainable tourism and safeguarding Bolivia’s PAs.
Round-the-Clock Adsorption–Degradation of Tetracycline Hydrochloride by Ag/Ni-TiO2
Authors: Ma Siyu , Qin Yiying , Sun Kongyuan , Ahmed Jahangeer , Tian Wei , Ma Zhaoxia
Published: June 2024

The synergy of adsorption and photocatalysis is a good method to remove organic pollutants in wastewater. In recent decades, persistent photocatalysis has gained considerable interest for its ability to sustain the catalytic degradation of organic pollutants in the dark. Herein, we report three different TiO2 nanomaterials to remove tetracycline hydrochloride (TCH) in solution. We found that the removal ability of TiO2, Ni-TiO2, and Ag/Ni-TiO2 is 8.8 mg/g, 13.9 mg/g and 23.4 mg/g, respectively, when the initial concentration of TCH is 50 mg/L. Chemical adsorption could be the rate-determining step in the TCH adsorption process. Moreover, Ag nanoparticles dispersed on Ni doped TiO2 surface act as traps to capture photo-generated electrons upon illumination with indoor light. The holes in Ag/Ni-TiO2 serve as critical oxidative species in TCH degradation under dark conditions. This work provides new insights into the design of persistent photocatalysts that can be activated by weak illumination and degrade organic pollutants in wastewater after sunset.
Trueness of Extraoral Digital Impressions for Full-Arch Implant Impressions—In Vitro Study
Authors: Sampaio-Fernandes Manuel António , Pinto Ricardo , Almeida Paulo Rocha , Sampaio-Fernandes Maria Margarida , Marques Duarte , Figueiral Maria Helena
Published: June 2024

Direct scanning of silicone impressions is a valid technique. However, studies in implant-supported rehabilitations are lacking. This in vitro study aims to compare the trueness of impressions obtained with two types of silicone and their corresponding stone casts, using two laboratory scanners in a full-arch implant rehabilitation. A master cast with six dental implants was scanned with a 12-megapixel scanner to obtain a digital master cast. Ten implant impressions were made using two silicones (Zhermack and Coltene) with the open-tray technique. The impressions and stone casts were scanned by two extraoral scanners (Identica T500, Medit; and S600 ARTI, Zirkonzhan). Trueness was assessed by comparing linear and angular distances in digital casts with the master cast. A p < 0.05 significance level was considered. The results showed that for the linear measurements, 72% were higher than the master cast measurements, and no consistent pattern was observed in the angular measurements. The greatest deviations were detected between the most posterior implants, with mean values ranging between 173 and 314 µm. No significant differences were found between scanners. However, differences were observed in the distances between silicones (46.7%) and between impressions and stone casts (73.3%). This work demonstrates that the direct scanning of silicone impressions yields results comparable to those obtained from scanning gypsum casts in full-arch implant-supported rehabilitation.
Bending Strength of Continuous Fiber-Reinforced (CFR) Polyamide-Based Composite Additively Manufactured through Material Extrusion
Authors: Łakomy Maciej , Kluczyński Janusz , Sarzyński Bartłomiej , Jasik Katarzyna , Szachogłuchowicz Ireneusz , Łuszczek Jakub
Published: June 2024

This paper shows the three-point bending strength analysis of a composite material consisting of polyamide doped with chopped carbon fiber and reinforced with continuous carbon fiber produced by means of the material extrusion (MEX) additive manufacturing technique. For a comparison, two types of specimens were produced: unreinforced and continuous fiber-reinforced (CFR) with the use of carbon fiber. The specimens were fabricated in two orientations that assure the highest strength properties. Strength analysis was supplemented by additional digital image correlation (DIC) analysis that allowed for the identification of regions with maximum strain within the specimens. The utilization of an optical microscope enabled a fractographic examination of the fracture surfaces of the specimens. The results of this study demonstrated a beneficial effect of continuous carbon fiber reinforcement on both the stiffness and strength of the material, with an increase in flexural strength from 77.34 MPa for the unreinforced composite to 147.03 MPa for the composite reinforced with continuous carbon fiber.
Improved Research on Two-Step Thermal Stress Calculation Method for Asphalt Mixture: Extended Creep Compliance Test
Authors: He Xu , Li Peng , Lin Bo , Jiang Shuangquan
Published: June 2024

The two-step thermal stress calculation method (TTSCM) is commonly used to predict the cracking temperature of asphalt mixture. The aim of this study is to improve TTSCM’s mathematical model so as to enhance its prediction accuracy. First, this study evaluated the errors of predicted cracking temperatures of original TTSCM for AC-16 and AC-25 asphalt mixtures by thermal stress-restrained specimen test (TSRST). Then, an improved method called the extended creep compliance test (ECCT) was developed to modify the TTSCM. The test results show that the cracking predictions of the original TTSCM are not always accurate. Particularly for AC-16 asphalt mixture, the predicted cracking temperature is 2.9 °C (−10.6%) higher than the measured value by the TSRST. The ECCT method has been proven to be an effective way to enhance the prediction accuracy of the TTSCM. The predicted cracking temperatures modified by the ECCT method for both asphalt mixtures are relatively accurate, having an error within ±2%. The ECCT method changed the calculated thermal stress values at different temperatures of the TTSCM; however, they still conformed to a basic changing trend with respect to the initial temperature and cooling rate. Finally, a recommendation regarding the ECCT method was presented.
Synthesis and Characterization of Polyaniline Emeraldine Salt (PANI-ES) Colloids Using Potato Starch as a Stabilizer to Enhance the Physicochemical Properties and Processability
Authors: Boudjelida Soufiane , Li Xue , Djellali Souad , Chiappetta Giampiero , Russo Francesca , Figoli Alberto , Carraro Mauro
Published: June 2024

Conductive polymers, such as polyaniline (PANI), have interesting applications, ranging from flexible electronics, energy storage devices, sensors, antistatic or anticorrosion coatings, etc. However, the full exploitation of conductive polymers still poses a challenge due to their low processability. The use of compatible stabilizers to obtain dispersible and stable colloids is among the possible solutions to overcome such drawbacks. In this work, potato starch was used as a steric stabilizer for the preparation of colloidal polyaniline (emeraldine salt, ES)/starch composites by exploiting the oxidative polymerization of aniline in aqueous solutions with various starch-to-aniline ratios. The polyaniline/starch bio-composites were subjected to structural, spectroscopic, thermal, morphological, and electrochemical analyses. The samples were then tested for their dispersibility/solubility in a range of organic solvents. The results demonstrated the formation of PANI/starch biocomposites with a smaller average size than starch particles, showing improved aqueous dispersion and enhanced solubility in organic solvents. With respect to previously reported PANI-EB (emeraldine base)/starch composites, the novel colloids displayed a lower overall crystallinity, but the conductive nature of PANI-ES enhanced its electrochemical properties, resulting in richer redox chemistry, particularly evident in its oxidation behavior, as observed through cyclic voltammetry. Finally, as proof of the improved processability, the colloids were successfully integrated into a thin polyether sulfone (PES) membrane.