PENGARUH AUKSIN IBA DAN NAA TERHADAP INDUKSI PERAKARAN INGGU
Authors: SYAHID SITTI FATIMAH , KRISTINA NATALINI NOVA 
Published: June 2020

<p><span style="font-family: Calibri; font-size: medium;">ABSTRAK</span></p><p><span style="font-family: Calibri; font-size: medium;"> </span></p><p><span style="font-family: Calibri; font-size: medium;">Inggu (Ruta graveolens L.) merupakan salah satu tanaman obat <br /> </span><span style="font-family: Calibri; font-size: medium;">langka di Indonesia yang perlu dilestarikan. Upaya konservasi tanaman <br /> </span><span style="font-family: Calibri; font-size: medium;">inggu telah dilakukan secara in vitro di laboratorium Balittro selama 17 <br /> </span><span style="font-family: Calibri; font-size: medium;">tahun pada kultur tunas. Untuk mengobservasi kestabilan genetik perlu <br /> dilakukan induksi perakaran. Penelitian ini bertujuan untuk mengetahui <br /> </span><span style="font-family: Calibri; font-size: medium;">pengaruh auksin IBA dan NAA terhadap induksi perakaran inggu secara in <br /> </span><span style="font-family: Calibri; font-size: medium;">vitro. Bahan tanaman yang digunakan adalah tunas steril inggu in vitro <br /> </span><span style="font-family: Calibri; font-size: medium;">yang telah berumur 17 tahun, yang ditanam pada media dasar Murashige <br /> </span><span style="font-family: Calibri; font-size: medium;">dan Skoog (MS) setengah konsentrasi (½ MS) yang diperkaya vitamin dari <br /> </span><span style="font-family: Calibri;"><span style="font-size: medium;">group B. Perlakuan yang diuji adalah beberapa taraf konsentrasi auksin <br /> IBA dan NAA (0; 0,001; 0,002; dan </span></span><span style="font-family: Calibri; font-size: medium;">0,003 mg/l). Rancangan yang <br /> </span><span style="font-family: Calibri; font-size: medium;">digunakan adalah Rancangan Acak Lengkap dengan lima ulangan. Setiap <br /> </span><span style="font-family: Calibri; font-size: medium;">ulangan terdiri dari lima botol yang berisi dua tanaman. Parameter yang <br /> </span><span style="font-family: Calibri; font-size: medium;">diamati adalah jumlah, panjang, dan bentuk akar, serta jumlah tunas dan <br /> </span><span style="font-family: Calibri; font-size: medium;">penampilan kultur. Hasil penelitian menunjukkan bahwa penggunaan <br /> </span><span style="font-family: Calibri; font-size: medium;">media ½ MS yang diperkaya NAA pada konsentrasi rendah 0,001 mg/l <br /> </span><span style="font-family: Calibri;"><span style="font-size: medium;">menghasilkan jumlah akar terbanyak, yaitu 13,6 akar. Perlakuan ini juga <br /> menghasilkan banyak bulu-bulu akar yang menandakan akar yang sehat.</span></span></p><p><span style="font-family: Calibri; font-size: medium;"> </span></p><p><span style="font-family: Calibri; font-size: medium;">Kata kunci: Ruta graveolens L., IBA, NAA, induksi perakaran, in vitro</span></p><p><span style="font-family: Calibri; font-size: medium;"> </span></p><p> </p><p><span style="font-size: medium;">The Effect of Auxin IBA and NAA to In Vitro Rooting Induction of Roe (Ruta graveolens L.) </span></p><p><span style="font-family: Calibri; font-size: medium;"> </span></p><p><span style="font-family: Calibri; font-size: medium;">ABSTRACT</span></p><p><span style="font-family: Calibri; font-size: medium;">Roe (Ruta graveolens L.) is one of the Indonesian rare medicinal <br /> </span><span style="font-family: Calibri; font-size: medium;">plants. An attempt to conserve roe, has been conducted through in vitro <br /> </span><span style="font-family: Calibri; font-size: medium;">culture of sterile shoots at the laboratory of the Indonesian Spice and <br /> </span><span style="font-family: Calibri; font-size: medium;">Medicinal Crops Research Institut (ISMCRI) for 17 years. To observe the <br /> </span><span style="font-family: Calibri;"><span style="font-size: medium;">genetic stability of culture following in vitro conservation for a long <br /> period, the collection must be tested in greenhouse and field. Therefore, it <br /> is necessary to induce rooting. The aim of the experiment was to observe <br /> </span></span><span style="font-family: Calibri;"><span style="font-size: medium;">the effect of IBA and NAA auxin to root induction of roe. The sterile <br /> shoots were used as material. They were planted on half-concentration (½ <br /> MS) on Murashige and Skoog (MS) medium, enriched with vitamin from <br /> </span></span><span style="font-family: Calibri; font-size: medium;">group B. The experiment was arranged in a completely randomized design <br /> </span><span style="font-family: Calibri;"><span style="font-size: medium;">with five replications. Each replication consist of five bottles with two <br /> plants. The treatment tested were several concentrations of IBA and NAA <br /> (0; 0.001; 0.002; and 0.003 mg/l). The parameters observed were number, <br /> </span></span><span style="font-family: Calibri;"><span style="font-size: medium;">lenght, shape, and length of roots, and also the number of shoots and <br /> culture performance. The result showed that the use of ½ MS + NAA</span></span></p><p><span style="font-family: Calibri;"><span style="font-size: medium;">0.001 mg/l produced the highest number of roots  </span></span><span style="font-family: Calibri;"><span style="font-size: medium;">(13.6 roots). This <br /> treatment also produced a lot of root hairs which indicates a healthy roots.</span></span></p><p><span style="font-family: Calibri; font-size: medium;"> </span></p>Key words: Ruta graveolens L., IBA, NAA, roots induction, in vitro