bluuebunny/crossref_metadata_2025 huggingface dataset:
Cleavage of the Amino-Terminal Domain of Cd36 by Hiv-1 Protease
Authors: Greenwalt Dale E.
Published: June 2022

The HIV-I protease has been found to cleave the integral membrane protein CD36 at two specific sites (residues 43*44 and 67*68) in the amino-terminal region of the bovine protein. Neither cleavage site conforms to previously identified type I or type 2 sites characteristic of HIV-1. Human CD36, which differs only slightly in its amino acid sequence.Was not cleaved by the HIV-1 protease.
Purification, Crystallization and Preliminary X-Ray Diffraction Studies on Snake Venom Protein Acutin
Authors: Gong Weimin , Su Shaoyu , Niu Liwen , Teng Maikun , Xu Qingping
Published: June 2022

A new snake venom protein was purified from A gkistrodon acutus and named as Acutin. Acutin has a molecular weight of 30kDa and pi of 5.0. Crystals of Acutin have been grown using the vapor diffusion method. The crystals diffracted X-rays at 3.0A. The space group is R32 with H cell dimensions of a=b=I57.02A, c=77.43A, α.=β=90°, γ=l20°. There is one Acutin molecule in the crystallographic asymmetric unit.
Comparative Dynamic Features of Apo and Bound MDM2 Protein
Reveal the Mechanism of Inhibitor Recognition for Anti-Cancer
Activity
Authors: I. El habbash Aisha , El Rashedy Ahmed , Soliman Mahmoud E.S.
Published: June 2022

Background:

Mouse Double Minute 2 Homolog (MDM2) oncogenic protein is the principal cellular antagonist of the p53 tumor suppressor gene. Restoration of p53 activity by inhibiting the MDM2-P53 interactions at the molecular level has become the cornerstone of cancer research due to its promising anticancer effects. Natural medicinal products possess various chemical structures and represent an essential source for drug discovery. α-Mangostin (AM) and gambogic acid (G250) are plant-derived compounds that showed inhibitory effects on MDM2-P53 interactions in-vitro and in-vivo.

Methods:

Despite the many clinical studies which performed deeper insight about the molecular understanding of the structural mechanisms exhibited by α-Mangostin and Gambogic acid-binding to MDM2 remains critical. In this study, comparative molecular dynamics simulations were performed for each Apo and bound p53 and MDM2 proteins to shed light on the MDM2-p53 interactions and get a better understanding of the inhibition mechanisms.

Results:

Results revealed atomistic interaction of AM and G250 within the MDM2-p53 interaction cleft. Both compounds mediate the interaction between the α-helix motifs of the p53 amino-terminal domain. Which caused a significant separation between orthogonally opposed residues, specifically Lys8 and Gly47 residues of the p53 and MDM2, respectively. Contrasting changes in magnitudes were observed in per-residue fluctuation on AM and G250 (~0.04 nm and ~2.3 nm, respectively). The Radius of gyration (~0.03 nm and 0.04 nm, respectively), C-alpha deviations (~0.06 nm and 0.1 nm, respectively). The phenolic group of AM was found to establish hydrogen interactions with Glu28 and His96 residues of MDM2. The trioxahexacyclo-ring of G250 also forms hydrogen bond interactions with Lys51 and Leu26 residues of MDM2.

Conclusion:

Utilizing the information provided on the inhibitory binding mode adopted by each compound in this study may further assist in the tailored designs for cancer therapeutics.
A Prospective Study on the Incidence and Severity of Paclitaxel-induced
Peripheral Neuropathy in the Indian Population
Authors: Reghu R. , George A.M. , Arya M.A. , Joseph S.K. , Philip A. , Sam K.M.
Published: June 2022

Background:

Despite the high efficacy rate of paclitaxel, physicians are compelled to
discontinue the regimen due to its prevailing neurotoxicity and myelosuppressive effects, thus not
achieving the desired clinical outcomes. The neurotoxicity studies of paclitaxel have been mostly
performed on upper dose limits (>275mg/m2), and little information is available on lower doses.
Since there is a lack of such studies on the Indian population, the medical professionals are unable to
analyze at what cumulative dose does paclitaxel show maximum severity of peripheral neuropathy.

Methods:

This is a prospective observational study conducted for 1 year in patients undergoing
paclitaxel therapy. These patients were evaluated for the incidence and severity of paclitaxel-induced
peripheral neuropathy during the first 6 cycles using the QLQ-CIPN questionnaire. We also identified
the cumulative dose at which most patients developed peripheral neuropathy and each patient’s
quality of life using EORTC QLQ C30.

Results:

Out of 85 patients, 76 developed peripheral neuropathy during the first 6 cycles. It was
observed that the severity of peripheral neuropathy increased in each cycle of therapy. The overall
quality of life of patients decreased with therapy, and at a cumulative dose of 525mg/m2, most of the
patients (40%) developed symptoms of peripheral neuropathy.

Conclusion:

The incidence and severity of peripheral neuropathy increased with each cycle, leading
to a significant reduction in the quality of life of patients post 6 cycles. Moreover, a high cumulative
dose may limit the paclitaxel therapy.
Deep Eutectic Solvent (DES) Mediated Multicomponent Synthesis of 4-
thiazolidinone-5-carboxylic Acid: A Green Chemistry Approach
Authors: Shaikh Majid , Shaikh Mujahed , Wagare Devendra , Ahmed Sheikh Anis , Sultan Kasim Sayyad
Published: June 2022

Background:

The 4-thiazolidinone-5-carboxylic acid and its derivatives have diverse
applications in agriculture, industrial and pharmaceutical fields. Therefore, the synthesis of this
heterocyclic compound attracted much attention from researchers with green chemistry protocols.
In this research work, we have introduced the green protocol for the synthesis of 4-thiazolidinone-
5-carboxylic acid by keeping the parameters in mind like cost-effective, environmentally benign,
short reaction time and easy work-up procedure.

Methods:

Initially, we irradiated the mixture of substituted aldehyde, thiosemicarbazide and furan
2-5-dione in the presence of choline chloride-thiourea-based Deep Eutectic Solvent [DES] as a
green medium. The reaction optimization was performed in different solvents like ethanol, glycerol,
and PEG-400.

Results:

The DES, which was used as a green solvent, produced an excellent result in context to
short reaction time, yield, easy workup, mild reaction condition and cost-effective protocol. All the
results are discussed.

Conclusion:

The DES-mediated synthesis of 4-thiazolidinone-5-carboxylic acid is found to be an
excellent protocol, which followed green chemistry principles. This method has specific features
like mild reaction conditions, environmentally benign, cost-effective and easy workup procedure.
An Overview of Biomaterial Toxicity and Excretion
Authors: Sharma Srijana , Mishra Yachana , Bisht Shubham , Sharma Neha , Mishra Vijay
Published: June 2022

Biomaterial is a growing family of materials with specific physicochemical
properties. Significant studies have been made to characterize the potential in vivo and
in vitro toxicity of biomaterials. The cytotoxicity may be attributed to variations in the
physicochemical properties, target cell types, particle dispersion methods, etc. The
reported cytotoxicity effects mainly include the impact on the biological system and
organ-specific toxicity such as CNS toxicity, lung toxicity, cardiac toxicity, dermal
toxicity, gastrointestinal toxicity, etc. Despite cellular toxicity, the immunological
effects of biomaterials, such as the activation of pulmonary macrophages and
associated inflammation, have been extensively studied. In this chapter, the latest
research results on the toxicological profiles of nanomaterials, highlighting both the
cellular toxicities and the immunological effects, have been incorporated. This analysis
also offers details on the overall status, patterns, and research needs for dealing with
the toxicological behavior of biomaterials.
Magnetic Nanoparticles for Imaging, Diagnosis, and Drug-Delivery Applications
Authors: Abu-El-Rub Ejlal , Zegallai Hana M. , Aloud Basma Milad , Sekaran Saravanan , Miller Donald W.
Published: June 2022

Magnetic Nanoparticles (MNPs) have gained interest within the research
community due to their therapeutic potential in a variety of medical applications.
MNPs are generally composed of a metallic core stabilized by the addition of an outer
shell that can be further functionalized through the absorbance or conjugation of
various targeting ligands. The magnetic properties of these nanoparticles can be
utilized for imaging, localized drug delivery, and enhanced diagnostic detection. This
chapter highlights the applications of MNPs to enhance magnetic resonance imaging
(MRI) capabilities and improve the delivery of therapeutic agents to difficult-to-reach
areas in the body. In addition, recent advances in the use of MNPs in stem cell therapy
for both the tracking and monitoring of stem cell distribution in the body and
improving engraftment and differentiation in stem cell therapy are discussed. Finally,
examples of the incorporation of MNPs in diagnostic assays to improve rapid and realtime detection capabilities of many diseases, including cancer, cardiovascular diseases,
and pathogen infections, are provided.
Viral and Non-viral Nanoparticles for Gene Therapeutics
Authors: Elsana Hassan , Elkordy Amal Ali
Published: June 2022

The recent accomplishment of the human genome and DNA discovery has led to the diagnosis of many diseases caused by imperfections in genes. These diseases involve gross disturbances in the number or arrangement of a person's chromosomes. Hence, gene therapy has become a promising new therapy for the treatment of somatic diseases, for example, malignant tumours [1], severe infectious diseases, such as AIDS [2], and many genetic disorders, including haemophilia and cystic fibrosis [3]. Gene therapy introduces a gene into human cells to replace, delete, or correct gene function to produce a therapeutic protein with the desired action. This adjustable gene can be used to cure any disease. In 1990, a gene therapy clinic was initiated to find treatment for severe combined immunodeficiency (SCID). However, the first success of gene therapy was not observed until 2000 when Cavazzana calvo et al. [4] reported a success using gene therapy for the treatment of SCID [4]. While it has been 30 years since the first gene therapy trial, gene therapy is still a high-risk treatment, and only a few drugs have been approved, such as Glybera® , Gendicine®, and Strimvelis®.
La sporulation du martyre (XVie et XXIe siècles)
Authors: Bourgaux Aurélien
Published: June 2022

Le martyre constitue un sujet omniprésent dans notre actualité. Or le discours sur la mort pour une cause vise de tout temps à promouvoir le caractère exemplaire du sacrifice. Cette donnée pose la question de la propagation de cette conception : comment le martyre se diffuse-t-il et de quelles représentations sa transmission faitelle l’objet ? Cet article propose un examen des modalités de « sporulation » du martyre à la fois dans la confession protestante au XVIe siècle et de nos jours à travers le cas du terroriste d’extrême droite Anders Behring Breivik. À l’instar de la littérature en histoire moderne parue à la suite des récents attentats de Paris et de Bruxelles, il s’agira de mettre en lumière, au départ de son étude dans le passé, un schéma de compréhension de nos phénomènes contemporains
Illegale handel van cultuurgoederen als financieringsbron van terrorisme
Authors: Desmet Mathias
Published: June 2022

De systematische plunderingen die landen als Irak, Afghanistan en Syrië al decennialang moeten ondergaan, zijn gelinkt aan een vorm van transnationale georganiseerde misdaad die enorme opbrengsten kan opleveren: illegale handel in cultuurgoederen. Ook terroristische organisaties zagen daarin een lucratieve mogelijkheid om hun oorlogsvoering te financieren. Cultuurgoederen werden massaal uit musea en archeologische sites geplunderd en het land uit gesmokkeld. Door de grootschalige vernielingen, plunderingen en verhandeling van cultuurgoederen waar Islamitische Staat zich in Syrië en Irak schuldig aan maakte, kwam er meer aandacht voor dit criminaliteitsfenomeen. Dit behoeft echter nog verder onderzoek met het oog op beleidsvorming. Naast de organisatorische kenmerken van dat criminaliteitsfenomeen is het in grote mate onbekend wat de opbrengsten waren die terreurbewegingen daaruit gehaald hebben. Zeer uiteenlopende, vaak verkeerde, cijfers doen daarover de ronde en maken de nood aan diepgaand empirisch onderzoek duidelijk – ook in België.
ІНСУЛІНОРЕЗИСТЕНТНІСТЬ ТА ЇЇ ВЗАЄМОЗВ’ЯЗКИ З КАРДІО-МЕТАБОЛІЧНИМИ ФАКТОРАМИ У ХВОРИХ НА АРТЕРІАЛЬНУ ГІПЕРТЕНЗІЮ З ОЖИРІННЯМ
Authors: Коваль Сергій , Снігурська Ірина , Мисниченко Ольга , Літвінова Ольга
Published: June 2022

Мета:вивчення взаємозв'язків інсулінорезистентності (ІР) з кардіо-метаболічними факторами у хворих на артеріальну гіпертензію (АГ) з абдомінальним ожирінням (АО).
Матеріали та методи. В дослідження включено 86 хворих (50 чоловіків і 36 жінок) на есенціальну АГ 1-2-3 ступеня (І-ІІ стадій) з АО I-II ступеня віком від 40 до 59 років. Обстеження хворих проводили з використанням стандартних клінічних, лабораторних та інструментальних методів. ІР визначали шляхом розрахунку індексу НОМА і при значенні даного індексу >2,77 діагностували наявність ІР. Аналіз даних проведений за допомогою статистичної обробки з використанням комп'ютерної програми SPSS 19.0. для Windows XP.
Результати. Встановлено, що у переважної більшості хворих на АГ з АО (72%) діагностувалась ІР. Серед хворих на АГ з АО та з ІР в порівнянні з хворими з нормальною чутливістю до інсуліну, виявлені не тільки достовірно більша вираженість ожиріння та частота метаболічних порушень (переддіабета, комбінованої дисліпідемії та знижених рівнів холестерину ліпопротеїнів високої щільності), а й більш важкий перебіг АГ: достовірно більша частота АГ 3 ступеня і ІІ стадії, а також уражень органів-мішеней: гіпертрофії лівого шлуночка, зокрема концентричної, потовщення комплексу інтіма-медія загальних сонних артерій та хронічної хвороби нирок ІІІа стадії.
Висновки. Інсулінорезистентність у хворих на АГ з ожирінням є патогенетично вагомим фактором як розвитку і прогресування порушень вуглеводного і ліпідного обмінів, так і артеріальної гіпертензії.
46,XX/46,XY Chimerism & Human Sexual Development
Authors:   Keck Graduate Institute, Claremont, CA, USA, Tate Jason W. , Hara Bruce F. O’ ,   Department of Biology, University of Kentucky, Lexington, KY, USA, Wei Sainan ,   Department of Pathology and Laboratory Medicine, University of Kentucky, Lexington, KY, USA
Published: June 2022

The term chimera refers to an organism with cell lines from two or more distinct zygotes. Human chimerism may occur naturally or artificially. Although rare, advancements in genetics and genomics have resulted in the identification of additional natural human chimeras. Three forms of naturally occurring chimerism have been documented in humans: blood group chimerism, microchimerism, and fusion chimerism. Fusion chimerism may occur through several means. Sex-chromosome discordant chimerism refers to individuals with both XX and XY cell lines. There is a large amount of phenotypic variability among 46,XX/46,XY chimeric individuals. The care of people with intersex traits or DSD (Disorders of Sexual Development) is controversial due in part to a history of unnecessary surgical intervention and power-imbalances between the intersex and medical communities. As more 46,XX/46,XY chimeric individuals with intersex traits or DSD are identified, the implications for their care will need to be clarified.
Identifying the Risk of Sepsis in Patients With Cancer Using Digital Health Care Records: Machine Learning–Based Approach (Preprint)
Authors: Yang Donghun , Kim Jimin , Yoo Junsang , Cha Won Chul , Paik Hyojung
Published: June 2022

BACKGROUND

Sepsis is diagnosed in millions of people every year, resulting in a high mortality rate. Although patients with sepsis present multimorbid conditions, including cancer, sepsis predictions have mainly focused on patients with severe injuries.

OBJECTIVE

In this paper, we present a machine learning–based approach to identify the risk of sepsis in patients with cancer using electronic health records (EHRs).

METHODS

We utilized deidentified anonymized EHRs of 8580 patients with cancer from the Samsung Medical Center in Korea in a longitudinal manner between 2014 and 2019. To build a prediction model based on physical status that would differ between sepsis and nonsepsis patients, we analyzed 2462 laboratory test results and 2266 medication prescriptions using graph network and statistical analyses. The medication relationships and lab test results from each analysis were used as additional learning features to train our predictive model.

RESULTS

Patients with sepsis showed differential medication trajectories and physical status. For example, in the network-based analysis, narcotic analgesics were prescribed more often in the sepsis group, along with other drugs. Likewise, 35 types of lab tests, including albumin, globulin, and prothrombin time, showed significantly different distributions between sepsis and nonsepsis patients (P<.001). Our model outperformed the model trained using only common EHRs, showing an improved accuracy, area under the receiver operating characteristic (AUROC), and F1 score by 11.9%, 11.3%, and 13.6%, respectively. For the random forest–based model, the accuracy, AUROC, and F1 score were 0.692, 0.753, and 0.602, respectively.

CONCLUSIONS

We showed that lab tests and medication relationships can be used as efficient features for predicting sepsis in patients with cancer. Consequently, identifying the risk of sepsis in patients with cancer using EHRs and machine learning is feasible.
Parent Preferences for Peer Connection in eHealth Programs (Preprint)
Authors: Rioux Charlie , Konkin Avaline , MacKinnon Anna L , Cameron Emily E , Tomfohr-Madsen Lianne M , Watts Dana , Roos Leslie E
Published: June 2022

BACKGROUND

Social support and connection with other parents are important factors associated with parental mental health and parenting practices. These social connections can be integrated in parental eHealth programs through forums or group therapy sessions, but parental needs and preferences regarding these eHealth features are unknown.

OBJECTIVE

This study aims to examine parents’ preferences for connecting with other parents in eHealth programs.

METHODS

In total, 162 parents of 0-5–year-old children in the United States were recruited through Amazon Mechanical Turk (MTurk; mean age 32.7, range 22-61 years; 80.2% White; 59.9% men, 39.5% women, and 0.6% nonbinary; 93.8% biological parents). Participants filled out a one-time survey. Best practice recommendations for using MTurk were employed (through captcha verification and attention checks). Descriptive statistics were run in SPSS (version 27; IBM Corp) on MacOS.

RESULTS

Parents were asked to rate how likely they would be to use a digital program with weekly opportunities to connect with other parents in the program (1=very unlikely to 5=very likely). Overall, 13.4% of parents indicated that they would be (very) unlikely to use a program with that feature and 59.8% of them indicated that they would be (very) likely to use it, with the remaining 27.8% of them being neutral. On being asked specifically about their preference, 85% of parents indicated that they would prefer connecting with other parents in the program, with 70% of those preferring to connect anonymously. On a forum, 67% of parents indicated that they would be comfortable connecting with all parents (as opposed to mothers or fathers only); regarding videoconferencing, that number was 61%.

CONCLUSIONS

Considering that studies have shown the positive impact of social support for parental mental health and parenting practices, integrating anonymous connection with other parents should be considered in developing parental eHealth programs and would be in line with the preferences of most parents.
Low- and Medium Socio-economic Status Group Members’ perceived challenges and solutions for (technology for) healthy nutrition: A qualitative focus group study (Preprint)
Authors: Hermsen Sander , van Kraaij Alex , Camps Guido
Published: June 2022

BACKGROUND

Although digital tools for healthy nutrition have shown great potential, their actual impact remains variable, because digital solutions often do not fit users’ needs and barriers. This is especially poignant for priority communities in society. Involving these groups in Citizen Science may have great benefits, even beyond the increase of knowledge about the lives and experiences of these groups. However, this requires specialised skills. Participants from priority groups could benefit from an approach that offers sensitization, gentle and safe provocation, and discussion, to help them voice their needs regarding (digital technology for) healthy nutrition.

OBJECTIVE

The study aims to gather insights on people’s thoughts on (applying technological innovations for) everyday eating practices, in self-regulation (for instance of snacking), and skill acquisition.

METHODS

Participants answered three daily questionnaires, to garner their current practices regarding habits, self-regulation, skills, and technology use surrounding healthy eating, and to make it easier for them to collect their thoughts and experiences (sensitisation). Within a week after filling out the three questionnaires, participants took part in an online focus group discussion session. Part of this discussion was a brief ‘provotyping’ exercise in which participants were presented with digital solutions for measuring health determinants. All sessions were transcribed and analysed using a thematic qualitative approach.

RESULTS

42 people took part in 7 focus group interviews of six people each. The analysis showed that participants would like to receive support from technology for a broad range of aspects of nutrition, such as in measuring the effect their personal nutrition has on their individual health; providing them with reliable product information; giving them practical guidance for healthy eating and snacking; and reducing the burden of registering food intake. Technology should be easy to use, reduce burdens, and be tailored to personal situations. Privacy and cost are major concerns to the participants.

CONCLUSIONS

The present study shows that people from low and medium-socioeconomic status groups have a need for specific support in tailoring their knowledge of healthy nutrition to their own situation, and see technology as a means to achieve this.
Technologies for Interoperable Internet of Medical Things Platforms to Manage Medical Emergencies in Home Care and Prehospital Care: Protocol for a Scoping Review (Preprint)
Authors: Seth Mattias , Jalo Hoor , Högstedt Åsa , Medin Otto , Björner Ulrika , Sjöqvist Bengt Arne , Candefjord Stefan
Published: June 2022

BACKGROUND

Population growth and aging have highlighted the need for more effective home care and prehospital care. Interconnected medical devices and applications, an infrastructure referred to as the Internet of Medical Things (IoMT), have enabled remote patient monitoring and can be important tools to cope with these demographic changes. However, developing IoMT platforms requires profound knowledge about clinical needs and challenges related to interoperability and how these can be managed with suitable technologies.

OBJECTIVE

The purpose of this scoping review is to summarize the best practices and technologies used to overcome interoperability concerns in IoMT platform development for medical emergencies in home care and prehospital care.

METHODS

This scoping review will be conducted in accordance with Arksey and O’Malley’s five-stage framework. Only peer-reviewed articles published in English will be considered. The databases/web search engines that will be used are IEEE Xplore, PubMed, Scopus, Google Scholar, National Center for Biotechnology Information, SAGE journals and ScienceDirect.

The search process for relevant literature will be divided into four different steps. This will ensure that a suitable approach is followed in terms of search terms, limitations and eligibility criteria. Relevant articles that meet the inclusion criteria will be screened in two stages: abstract and title screening and full-text screening.

RESULTS

The results of the preliminary searches indicate that there is sufficient literature to form a good foundation for the scoping review. The main clinical focus is the prevention and management of falls, and other medical emergencies, such as heart disease and stroke, are also considered.

CONCLUSIONS

This scoping review will provide insights and recommendations regarding how interoperable real-time IoMT platforms can be developed to handle medical emergencies in home care and prehospital care. The findings of this research could be used by researchers, clinicians, and implementation teams and facilitate future development and interdisciplinary discussions.
Batik Pacitan: Kontinuitas dan Perubahannya
Authors: Setiyoko Nanang
Published: June 2022

The main objective of this research is to explore and observe the existence, development, and changes of Pacitan batik, especially in the aspect of form, function and product of Pacitan batik in social, cultural and economic contexts. This research is descriptive qualitative by using a multidisciplinary approach, culture, aesthetics in the hope that it can answer all the problems. Changes caused by internal factors can occur due to new interpretations and perceptions of the Pacitan community, as a community that supports batik cloth. Pacitan people have a sense to participate in the development, innovation, ideas, and new creations of Pacitan batik. Changes caused by external factors generally occur because of relationships, communication, and social and cultural interactions with other cultures. Pacitan batik products have also experienced developments and changes, both in quantity and quality. Broadly speaking, the changes that occurred in Pacitan batik were marked by a change in its function, from the beginning as a long cloth worn by women during official events or during celebrations, to now becoming a trade commodity product. The motifs applied to Pacitan batik initially contained deep philosophical symbolic meanings, but now these meanings have been put aside, and only evaluated for their aesthetic aspects. Changes in cultural products will have an impact on the supporting community, both in terms of socio-cultural aspects, as well as socio-economic aspects.
Restriction Mapping of MC4R Gene on Bali Cattle (Bos sondaicus) as Genetic Marker for Breeding Program in Compared to Bos taurus and Bos indicus
Authors: Perdana Yoga Cipta , Hartatik Tety
Published: June 2022

MC4R is a gene that has potential effects on growth traits such as body weight and feed intake. The usage of single nucleotide polymorphism (SNPs) in melanocortin-4 receptor (MC4R) as selection markers could help achieve effectiveness in the breeding program. This study aimed to analyze the restriction mapping based on SNPs in the MC4R gene for Bali cattle (Bos sondaicus) compared to various breeds of cattle. Partial MC4R gene was amplified using a primer (F: 5’-ACC AAT GTC AGT GAG TCC CC- 3’ and R: 5’-CTT CAT GTT GGC GCC CTG-3’) with a polymerase chain reaction (PCR) method. Genotype and allele frequencies were calculated using Chi-Square test and analyzed with Hardy–Weinberg law. Restriction enzyme was analyzed using Nebcutter V.2 to see the association between SNPs and the recognition site of restriction enzyme. The result showed four SNPs g.554 T>C, g.634 G>T, g.673 C>T, and g.742 G>A were found in the exon region. SNP g.742 G>A was found as a heterozygote genotype and the rest are SNP g.554 T>C, g.634 G>T, and g.673 C>T were found as homozygote genotypes. All SNPs were synonymous which did not change the amino acid translated. Three restriction enzymes were identified as MmeI, TspRI, and BsrI which attach to SNPs g.554 T>C, g.634 G>T, and g. 742 G>A respectively.  SNPs found notably g. 742 G>A can be used as genetic markers associated with growth traits for further research on Bali cattle.
Minat Siswa Sekolah Menengah Kejuruan untuk Bekerja dalam Bidang Pertanian di Provinsi Jawa Tengah
Authors: Handayani Ari Widya , Hariadi Sunarru Samsi , Andarwati Siti
Published: June 2022

Tantangan bidang pertanian dewasa ini adalah ketersediaan sumber daya manusia. Rendahnya minat pemuda untuk terjun di sektor pertanian terutama sektor tanaman pangan dan hortikultura menjadi salah satu persoalan ketersediaan sumber daya manusia di bidang pertanian. Turunnya jumlah tenaga kerja di bidang pertanian dan minat pemuda untuk terjun di bidang pertanian akan berpotensi menyebabkan penurunan efisiensi dan produktivitas hasil pertanian. Penelitian ini bertujuan untuk mengidentifikasi minat siswa SMK Pertanian pasca lulus untuk bekerja di bidang pertanian beserta faktor-faktor yang memengaruhi serta mendeskripsikan upaya peningkatan minat siswa SMK untuk bekerja bidang pertanian. Penelitian dilaksanakan di 5 sekolah menengah kejuruan negeri (SMKN) yaitu SMKN 1 Trucuk, SMKN H Moenadi, SMKN 1 Bawen, SMKN 1 Wanareja dan SMK N 2 Slawi menggunakan metode survey. Populasi penelitian ini adalah siswa SMK kelas 11 dan 12 di 5 sekolah tersebut sejumlah 1295 orang. Pada masing–masing sekolah diambil 34 – 35 orang sebagai sampel menggunakan metode acak sederhana (simple random). Total sampel penelitian sebanyak 175 responden. Data dianalisis dengan uji regresi linier berganda. Hasil yang diperoleh menyatakan bahwa minat siswa dalam bekerja di bidang pertanian dipengaruhi oleh efikasi diri, sikap siswa terhadap sektor pertanian, dan pemanfaatan media sosial.
Geriatric Assessment in Elderly People by Using ABCDE (Ability Balance Cognitive Diseases Emotion) Method at Posyandu for Elderly People
Authors: Sulistyaningsih Nunik , Claramita Mora , Pramantara I Dewa Putu
Published: June 2022

Background: Elderly populations have increased in line with the increased life expectancy. Currently, the balance of disease burden on the elderly has shifted towards chronic disease. Cardiovascular disease, diabetes, cognitive impairment and depression are chronic diseases that cause suffering and death in elderly populations. Therefore, it is necessary to conduct a comprehensive and multidimensional health assessment as a preventive effort to reduce morbidity in the elderly.Objective: The general objective of this research was to obtain a description of elderly health status at the Posyandu for the elderly using a geriatric assessment called the ABCDE (Ability, Balance, Cognitive, Diseases, Emotion) method.Methods: This research used a cross-sectional design. The subjects were elderly persons ≥ 60 years old who participated in the Posyandu for the elderly and who had fulfilled inclusion and exclusion criteria. The estimated minimum sample size was 242 selected using stratified random sampling. The statistical analysis in this research employed univariate, and bivariate analysis using Chi square tests.Results: Elderly participants had light dependency (34.2%) with average activities of daily living (ADL) value of 19.39 ± 1.32, positive Romberg test 3.7%, and Sharpened Romberg Test 50%. The prevalence of cognitive impairment was 43.4%, while the average score of Mini Mental State Examination was 23.31 ± 6.08, hypertension prevalence 73.14%, hypercholesterolemia 13.22%, and diabetes mellitus 10.33%. Elderly who had depression and probable depression were 12.81%, consisting of mild depression 11.57% and moderate depression 1.24%. Balance disorders had a statistically significant correlation with ADL (p = 0.012; Ratio Prevalence (RP) = 1.422: 95% CI), cognitive impairment (p = 0.039; RP = 1.31: 95% CI), and depression (p = 0.034; RP = 1.63: 95% CI).Conclusion: The prevalences of hypertension, cognitive impairment, and balance disorder on sharpened Romberg test were high. There were statistically significant correlations between balance disorder and ADL, cognitive function, and depression. These findings support the application of geriatric assessment by using ABCDE which can be applied in primary care setting for elderly people.
Making and Validating a Promotive and Preventive Effort Model for Stages 1-5 of Chronic Kidney Disease in Primary Care Services
Authors: Hajam Anita Rahman , Djarwoto Bambang , Claramita Mora
Published: June 2022

Background: The prevalence of Chronic Kidney Disease (CKD) is continuously increasing worldwide, including in Indonesia and has become a global public health problem. With the increased number of patients with CKD and the limited number of nephrologists, primary care physicians and specialists should play an important role in providing quality sustainable prevention and treatment by improving promotive and preventive efforts in primary care. Most patients with CKD can be managed by doctors in primary care in collaboration with specialist doctors in secondary and tertiary care. However, clinical practice guidelines for promoting and preventing CKD in primary care apparently have not been optimally implemented.Objective: This research aimed to make a promotive and preventive model for stages 1-5 of CKD in primary care.Methods: This research employed a research and development strategy with the following stages: (1) Preliminary study stage (literature review), (2) Development stage (initial draft of the model) and (3) Resources validation stage (in-depth interviews).Results: Five primary care physicians, two internists and one nephrologist participated in the validation stage for the initial draft of the model. The three stages of this research obtained the pattern of doctor's practice for CKD and the feedback related to the model to be adjusted according to the condition of primary care in Indonesia.Conclusion: There is a lack of understanding and awareness of primary care physicians about prevention and treatment of CKD, and a lack of supporting examination facilities for serum creatinine and albuminuria in primary care for screening of the risk factors of CKD. A promotional and preventative model of stages 1-5 for CKD in primary care was successfully made and validated.
Profiles of Housewives with HIV in Surabaya
Authors: Silitonga Hanna Tabita Hasianna , Adrianto Hebert , Wartiningsih Minarni , Angriyanto Chindy Claudya
Published: June 2022

Most HIV transmission to housewives was transmission from husbands infected with HIV. The number of housewives infected by HIV was increasing especially in Surabaya. This research aimed to find out the profile of housewives infected with HIV so that strategies can be proposed to prevent transmission to this group. This was a descriptive study with cross-sectional design. The research subjects were housewives with HIV in Surabaya with purposive sampling method. Inclusion criteria were not commercial sex workers (CSWs) or former CSWs, had main role as housewives, one-time marriage history, can read, write, and willing to be respondents. The number of respondents were 58 people. Data was collected by questionnaire containing general characteristics, marital history, husband's history, signs and symptoms of HIV for the first time, history of the first examination, history of disclosure of first time status, and the first source of information about HIV. Data analysis was frequency distribution and percentage. The results showed that the age of majority of respondents were 36-45 years old, high school education, married age 17-25 years, husband's age at marriage 17-25 years, husband's work was an employee, with high school education, history of husband's behavior was having a relationship with other women, the history of the first HIV test was in hospital, with no symptoms, the first source of information about HIV was a doctor, and the majority revealed it to her husband. Proposed strategies in accordance with these profiles are interventions in high school, pre-marriage, marriage, and the husband's workplace setting.
CASE REPORT: Interprofessional Collaboration in the Management of Scabies Re-infestation: A Single Case Study in a Rural Area of East Nusa Tenggara Province, Indonesia
Authors: Say Maria Damayanti Gufra Putri , Arisanti Nita , Arya Insi Farisa Desy
Published: June 2022

Mr. A presented with complaints of itching and reddish patches on his stomach, palms and fingers for about three weeks. The patient had been diagnosed with scabies infestation three times before and had previously been declared cured.Dermatological exam on this patient showed erythematous papules, rounded, well-defined, discrete spread and multiple measuring 0.2 x 0.2 cm on patient’s hand fingers, palm and abdomen. Excoriation and lichenification were also noticed.The presence of cuniculi were no longer found and some parts had undergone lichenification, marked by darkened skin color due to chronic scratching.The patient is a clove trader who often goes from one village to another to collect cloves. Every time he stops in a village, the patient will stay at a relative's house. He never brings personal towels and gloves while traveling. Often the towels and sarongs that are used by the host are lent to him. He always borrows towels and sarongs from the owner of the house where he is staying. Every time he comes home from traveling, the patient will suffer from itching which causes his wife and children to also get similar itching.
Preface
Authors: Wonodirekso Sugito
Published: June 2022

Communication Skills and Cultural Competence:The mainstay of the Family Doctors to contribute to increasing national health status, patient safety, and patient satisfaction
Management of Thirst in Hemodialysis Patients in Primary Services
Authors: Lestari Sri , Kuswadi Iri , Prasanto Heru
Published: June 2022

The incidence and prevalence of chronic kidney disease (CKD) has increased worldwide and in Indonesia. There were about 53,940 people with stage 5 CKD based on data from the Indonesian Renal Registry (IRR) in 2018. Incidence crude rate is 251 per million population and prevalence crude rate is 499 per million population for the entire population1. The prevalence of CKD has almost doubled in 5 years, from 2‰ in 2013 to 3.8‰ in 2018. Hemodialysis (HD) is a renal replacement therapy modality that is in great demand by the public compared to the other two modalities, namely peritoneal dialysis and kidney grafts. In 2018, there was a consistent increase in the number of new patients and active HD patients. Active patients is the total number of patients (either new or old patients) who are still undergoing routine HD. The number of new patients has doubled compared to 2017. This also resulted in a sharp increase in the number of active patients compared to the previous year.
Preface
Authors: Fujiati Isti Ilmiati
Published: June 2022

Family Medicine and Transformation of Primary Health Care
A Correlational Study on Teachers’ Level of Competence on and Attitudes Towards ICT Integration: Basis for the Development of Extension Training Program
Authors: Beltran Hazel
Published: June 2022

The integration of ICT in teaching and learning processes brings about powerful learning environments with the recent shift in Blended Learning-Online Distance Learning and helps teachers to deal with knowledge in active, self-directed and constructive ways. The purpose of this study was to determine if the adapted theory of Reasoned Actions as presented by Ajzen and Fishbein (1980) and Technological Acceptance Model developed by Davis (1989) relates to teachers’ competence on and attitudes in the implementation of ICT to justify the interim guidelines on the across implementation of BL-ODL and MDL modalities by BE-Learning Continuity Plan of Department of Education as per memorandum of LDM1 and LDM2 in the current school year. As part of a pilot-testing program of the LGU, the researcher sought to develop an instrument that would effectively measure the teachers’ level of competence on and attitudes with the utilization of ICT. Two schools JHS and SHS were studied since they belong to the same cluster. The study included 25 teacher-respondents from KERISHS-SHS and 25 teacher-respondents from GABHS-FBSHS. Each respondent completed the survey questionnaire, and gathered the necessary data for each level of competence on and attitudes that could benefit the entire educational community. The researcher applied Pearson’s r correlation in order to determine if a significant correlation existed. The results of the study revealed to have a significant or strongly positive relationship data to support the study. This research may encourage administrators to recognize and utilize the availability of ICT computer-assisted laboratories and enhance the scope of internet connection in formal and informal learning environments available in the school district
САРАДЊА И ПАРТНЕРСКИ ОДНОСИ У ПРЕДШКОЛСКОМ ВАСПИТАЊУ – ЗАДОВОЉСТВО РОДИТЕЉА
Authors: Marković Marija , Stanisavljević Petrović Zorica
Published: June 2022

From the standpoint of a systemic approach, the paper considers the co-operation between families and pre-school institutions. It indicates the relevance and topicality of this issue in a time of reform which aims to build a quality pre-school education. The paper advocates the process supportive model of partnership between parents and pre-school teachers that places focus on the quality of their interrelations.The research aim was to examine parental satisfaction with the co-operation with pre-school teachers. Research results have shown a high degree of respondents’ agreement with the assertions relating to parental satisfaction with the co-operation with a pre-school institution. The research data obtained on a sample of parents with college and university degrees are indicative because they expressed the lowest level of satisfaction with such co-operation. However, respondents within this category have highly assessed the assertions relating to parental involvement in the work of a pre-school institution and pre-school teachers’ acknowledgment of parental recommendations, which is encouraging form the standpoint of developing a partnership between parents and pre-school teachers. Pedagogical implications of the research can be observed from a theoretical standpoint in terms of inspiring other researchers to further delve into relationships between family and the system of pre-school education and upbringing, especially from a standpoint of numerous determinants which shape those relations. Implications for the pedagogical practice are reflected in perceiving that the category of highly educated parents comprises a significant force that can initiate changes concerning the co-operation between parents and pre-school teachers in terms of establishing and developing partner relationships and other topical changes in the field of pre-school education and upbringing.
MEDIA CRISIS AS A MEASURE OF RESPONSIBILITY OF MEDIA EMPLOYEES
Authors: Penezić Slobodan , Cvetićanin Neven
Published: June 2022

Numerous challenges and problems the media have faced in recent years, followed by the disappearance of many, resulted in a diagnosis of their global crisis. This situation has forced many media practitioners and theorists to think more about the situation and actively work on its interpretation to find a strategy for survival. Many of them, however, do not bring success, but only increase the problem because they represent a shift from the original role of journalism and the quality of the journalism. Therefore, the reversal of this logic is imposed as a response to the accumulated problems, and attempt to end the crisis. It also stresses the importance of the responsibility of media employees, especially those who run and organize media work - management, and the media content as its consequences. The focus of this article, therefore, is to try to illuminate this often unjustly neglected context, pointing to its importance, and hence the possibility of formulating of a media model that would allow a long-term stability for the media today.
MY JOB IS TO ENJOY! – THE SERBIAN EXPERIENCE OF LIFESTYLE ENTREPRENEURSHIP
Authors: Nedeljković Marina , Čikić Jovana
Published: June 2022

The paper examines the recently recognized form of entrepreneurship – lifestyle entrepreneurship – in the specific context of (post)transitional society. The key question is: are there any specific characteristics of lifestyle entrepreneurship and lifestyle entrepreneurs (e.g. personal characteristics, business traits, motivation, business plans and strategies, etc.) due to the specific economic and social settings. The exploratory study covers six lifestyle entrepreneurs. Data were collected in in-depth interviews. Results proven that (post)transitional lifestyle entrepreneurship and entrepreneurs are specific, especially regarding their motivation and work-private life balance. (Post)transitional lifestyle entrepreneurship includes basic features of profit-orientated entrepreneurship as an element of survival strategy.
DEVELOPMENT OF CREATIVE ABILITIES THROUGH ARTS EDUCATION
Authors: Stojanović Stosic Milena
Published: June 2022

The paper examines possibilities for enhancing creativity in primary school children through Arts education. Specifically, this study investigates the impact of visual, auditory and tactile stimuli (VAT stimuli)[1] on the development of creative thinking in children when these aids are carefully selected and suitable for use for a particular teaching topic within the Arts education curriculum. The study was conducted in Serbia, through an experimental two-term programme called The Development of Creativity by Employing VAT Stimuli, which included 270 first and second-year primary school pupils (7-8 year-olds). The impact of the experimental programme was assessed according to the Torrance Test of Creative Thinking (TTCT) – figural form A. This study found that the use of VAT stimuli in Arts education contributed significantly to developing and cultivating pupils’ creative abilities, and that the effect of the programme had a stronger impact on the 2nd year pupils.[1] Visual stimulus – discussion and observation of an artwork as a stimulus for artistic expression; Auditory aid – instrumental composition as a stimulus for artistic expression; Tactile stimulus – finger painting.
FASCINATION WITH THE MODERNIZATION OF JAPAN – REACTIONS OF THE WEST-EUROPEAN PART OF THE SILK ROAD TROUGH THE PRISM OF REVERSIBLE EXOTISME
Authors: Golubovic Tasevska Milica
Published: June 2022

The interruption of Japan's foreign relations, which has lasted since the beginning of the 7th century, was interrupted by the signing of the treaty of Kanagawa and the seizure of power by the emperor Mutsuhito, who called his reign the Meiji period, or „Enlightened rule.” It was during this period that the foundations for modernization of today's sophisticated Japan were laid. Japan begins to open up to the world, but it retains its independence, combining tradition and modernism in all segments. The journey and the exchange of ideas that have inspired many literary works, not only by the French writer Pierre Loti, on whose work this paper is partially based, then artistic ideas and artefacts, aim to explore the author of this paper, which focuses on the presentation of elements of Japanese art and culture at the Exibition Universelle in Paris, 1867.The analytical-comparative method used in this paper shows the remarkable place of Japan in the cross-section that transported silk from the Mediterranean to East Asia, which has become a symbol of the connection between East and West and metaphors for various myths and legends from previous periods, and today the link between western and eastern cultural development models, whose proximity and distance are constantly changing, leaving a strong mark on all societies.