bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of 4-Week Inspiratory Muscle Training on Sport Performance in College 800-Meter Track Runners
Authors: Chang Yun-Chi , Chang Hsiao-Yun , Ho Chien-Chang , Lee Po-Fu , Chou Yi-Chen , Tsai Mei-Wun , Chou Li-Wei
Published: January 2021

Background and objectives: Respiratory muscle fatigue is one of the important factors limiting sports performance due to the metaboreflex. This reflex will cause a decrease in blood flow to the extremities and accelerate exercising limb fatigue. Previous studies found that inspiratory muscle training (IMT) can effectively enhance the respiratory muscle endurance and reduce fatigue during long-duration exercise or aerobic exercise, thereby enhancing athletic performance. However, the mechanism between inspiratory muscle strength, change of limb blood flow and sports performance still requires investigation, especially in short-duration exercise, anaerobic or both aerobic and anaerobic exercise. The purpose of this study was to investigate the effects of 4-week inspiratory muscle training on respiratory muscle strength, limb blood flow change rate and sports performance in recreational 800-m college runners. Materials and Methods: Twenty healthy 800-m college runners randomized into the IMT group (11 subjects) and control group (9 subjects). IMT consisted of 30 inspiratory efforts twice daily, 5 days a week, with intensity at 50%, 60%, 70% and 80% of maximum inspiratory pressure (MIP) for 4 weeks, while a control group kept 50% of MIP for 4 weeks. An 800-m trial test, limb blood flow change rate by using Impedance Plethysmography, and MIP were as the outcome measured variables and be evaluated. All measured variables were assessed before and after 4-week IMT training. Two-way ANOVA was conducted for statistical analysis. Results: The results showed significantly interaction between groups and pre-posttest. IMT group significantly decreased limb blood flow change rate from 19.91 ± 11.65% to 9.63 ± 7.62% after received the IMT training program (p < 0.05). The MIP significantly improved from 112.95 ± 27.13 cmH2O to 131.09 ± 28.20 cm H2O in IMT group, and the 800-m trial test also shorted the running time from 162.97 ± 24.96 s to 156.75 ± 20.73 s. But the control group no significantly changed in MIP and 800-m trial test. Conclusions: Our results indicated that the 4-week IMT training (twice a day, 5 days a week) significantly improves participants’ inspiratory muscle strength, 800-m running performance and decreases the limb blood flow change rate.
Biaxial Tensile Behavior of Commercially Pure Titanium under Various In-Plane Load Ratios and Strain Rates
Authors: Zhang Wei , Zhu Zhikang , Zhou Changyu , He Xiaohua
Published: January 2021

The aim of the present work is to contribute to the characterization of the biaxial tensile behavior of commercially pure titanium, under various in-plane loading conditions at room temperature, by a non-contact digital image correlation system. Several loading conditions, with load ratio ranging from 4:0 to 0:4 and displacement rate ranging from 0.001 to 0.1 mm/s, are examined. It is found that the yield strength and ultimate tensile strength of biaxial sample are greater than that of uniaxial sample, where the equi-biaxial sample shows the highest strength. It is also observed that increase in strain rate leads to remarkable improvement of tensile strength. Fractographic analysis indicates that the shape and size of dimples are load ratio and strain rate dependent. Additionally, a modified Johnson–Cook constitutive model was proposed to account for the effect of strain rate on biaxial tensile deformation. The experimental results are in good agreement with the simulated results, indicating that the proposed model is reliable to predict biaxial tensile deformation of commercially pure titanium at different strain rates.
Editorial for the Special Issue on Wide Bandgap Based Devices: Design, Fabrication and Applications
Authors: Medjdoub Farid
Published: January 2021

Emerging wide bandgap (WBG) semiconductors hold the potential to advance the global industry in the same way that, more than 50 years ago, the invention of the silicon (Si) chip enabled the modern computer era [...]
Measurement of Effects of Different Substrates on the Mechanical Properties of Submicron Titanium Nickel Shape Memory Alloy Thin Film Using the Bulge Test
Authors: Dang Nhat , Wang Zhao-Ying , Wu Ti-Yuan , Nguyen Tra , Lin Ming-Tzer
Published: January 2021

This study investigated the effects of different substrates on the mechanical properties of Ti-60at%Ni shape memory alloys (SMA). Two types of samples were prepared for this experiment: (1) a Ti-60at%Ni deposited on SiNx, and (2) a Ti-60at%Ni deposited on SiNx/Cr; both had a 600 nm thick film of Ti-60at%Ni. Deposition was done using the physical vapor deposition (PVD) process, and the microstructural changes and crystallization phase changes were observed through scanning electron microscopy (SEM) and X-ray diffraction (XRD). The results showed that the TiNi thin film with a Cr adhesion layer had better mechanical properties. The bulge test showed that TiNi thin film with a Cr adhesion had a higher Young’s modulus and lower residual stress. From the thermal cycling experiment, it was found that the Cr adhesion layer buffered the mismatch between TiNi and SiNx. Additionally, the thermal cycling test was also used to measure the thermal expansion coefficient of the films, and the fatigue test showed that the Cr layer significantly improved the fatigue resistance of the TiNi film.
Eoarchean to Neoproterozoic Detrital Zircons from the South of Meiganga Gold-Bearing Sediments (Adamawa, Cameroon): Their Closeness with Rocks of the Pan-African Cameroon Mobile Belt and Congo Craton
Authors: Kanouo Nguo Sylvestre , Kouske Arnaud Patrice , Ngueutchoua Gabriel , Venkatesh Akella Satya , Sahoo Prabodha Ranjan , Basua Emmanuel Archelaus Afanga
Published: January 2021

The core of detrital zircons from the southern Meiganga gold-bearing placers were analyzed by Laser Ablation Split Stream analytical techniques to determine their trace element abundances and U-Pb ages. The obtained data were used to characterize each grain, determine its formation condition, and try to trace the provenance. The Hf (5980 to 12,010 ppm), Y (27–1650 ppm), U (25–954 ppm), Th (8–674 ppm), Ti (2–256 ppm), Ta, Nb, and Sr (mainly <5 ppm), Th/U (0.06–2.35), Ti zircon temperature (617–1180 °C), ∑REE (total rare earth element) (98–1030 ppm), and Eu/Eu* (0.03 to <1.35) are predominant values for igneous crustal-derived zircons, with very few from mantle sources and of metamorphic origin. Crustal igneous zircons are mainly inherited grains crystallized in granitic magmas (with some charnockitic and tonalitic affinities) and a few from syenitic melts. Mantle zircons were crystallized in trace element depleted mantle source magmatic intrusion during crustal opening. Metamorphic zircons grown in sub-solidus solution in equilibrium with garnet “syn-metamorphic zircons” and in equilibrium with anatectic melts “anatectic zircons” during crustal tectono-metamorphic events. The U-Pb (3671 ± 23–612 ± 11 Ma) ages distinguish: Eoarchean to Neoproterozoic igneous zircons; Neoarchean to Mid Paleoproterozoic anatectic zircons; and Late Neoproterozoic syn-metamorphic grains. The Mesoarchean to Middle Paleoproterozoic igneous zircons are probably inherited from pyroxene-amphibole-bearing gneiss (TTGs composition) and amphibole-biotite gneiss, whose features are similar to those of the granites, granodiorites, TTG, and charnockites found in the Congo Craton, south Cameroon. The youngest igneous zircons could be grains eroded from Pan-African intrusion(s) found locally. Anatectic and syn-metamorphic zircons could have originated from amphibole-biotite gneiss underlying the zircon-gold bearing placers and from locally found migmatized rocks that are from the Cameroon mobile belt, which could be used as proxies for tracking gold.
Development and Validation of a Bioanalytical LC-MS/MS Method for Simultaneous Determination of Sirolimus in Porcine Whole Blood and Lung Tissue and Pharmacokinetic Application with Coronary Stents
Authors: Nguyen Thi-Thao-Linh , Duong Van-An , Vo Dang-Khoa , Jo Jeongae , Maeng Han-Joo
Published: January 2021

Sirolimus is a hydrophobic macrolide compound that has been used for long-term immunosuppressive therapy, prevention of restenosis, and treatment of lymphangioleiomyomatosis. In this study, a simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated for the simultaneous determination of sirolimus in both porcine whole blood and lung tissue. Blood and lung tissue homogenates were deproteinized with acetonitrile and injected into the LC-MS/MS system for analysis using the positive electrospray ionization mode. The drug was separated on a C18 reversed phase column with a gradient mobile phase (ammonium formate buffer (5 mM) with 0.1% formic acid and acetonitrile) at 0.2 mL/min. The selected reaction monitoring transitions of m/z 931.5 → 864.4 and m/z 809.5 → 756.5 were applied for sirolimus and ascomycin (the internal standard, IS), respectively. The method was selective and linear over a concentration range of 0.5–50 ng/mL. The method was validated for sensitivity, accuracy, precision, extraction recovery, matrix effect, and stability in porcine whole blood and lung tissue homogenates, and all values were within acceptable ranges. The method was applied to a pharmacokinetic study to quantitate sirolimus levels in porcine blood and its distribution in lung tissue following the application of stents in the porcine coronary arteries. It enabled the quantification of sirolimus concentration until 2 and 14 days in blood and in lung tissue, respectively. This method would be appropriate for both routine porcine pharmacokinetic and bio-distribution studies of sirolimus formulations.
Studies on the Enantioselective Synthesis of E-Ethylidene-bearing Spiro[indolizidine-1,3′-oxindole] Alkaloids
Authors: Yayik Nihan , Pérez Maria , Molins Elies , Bosch Joan , Amat Mercedes
Published: January 2021

A synthetic route for the enantioselective construction of the tetracyclic spiro[indolizidine-1,3′-oxindole] framework present in a large number of oxindole alkaloids, with a cis H-3/H-15 stereochemistry, a functionalized two-carbon substituent at C-15, and an E-ethylidene substituent at C-20, is reported. The key steps of the synthesis are the generation of the tetracyclic spirooxindole ring system by stereoselective spirocyclization from a tryptophanol-derived oxazolopiperidone lactam, the removal of the hydroxymethyl group, and the stereoselective introduction of the E-ethylidene substituent by acetylation at the α-position of the lactam carbonyl, followed by hydride reduction and elimination. Following this route, the 21-oxo derivative of the enantiomer of the alkaloid 7(S)-geissoschizol oxindole has been prepared.
Pretransitional Effects of the Isotropic Liquid–Plastic Crystal Transition
Authors: Drozd-Rzoska Aleksandra , Starzonek Szymon , Rzoska Sylwester J. , Łoś Joanna , Kutnjak Zdravko , Kralj Samo
Published: January 2021

We report on strong pretransitional effects across the isotropic liquid–plastic crystal melting temperature in linear and nonlinear dielectric response. Studies were carried out for cyclooctanol (C8H16O) in the unprecedented range of temperatures 120 K < T < 345 K. Such pretransitional effects have not yet been reported in any plastic crystals. Results include the discovery of the experimental manifestation of the Mossotti Catastrophe behavior, so far considered only as a hypothetical paradox. The model interpretations of experimental findings are proposed. We compare the observed pretransitional behavior with the one observed in octyloxycyanobiphenyl (8OCB), typical liquid crystal (LC), displaying a reversed sequence of phase transitions in orientational and translational degrees of order on varying temperature. Furthermore, in its nematic phase, we demonstrate first-ever observed temperature-driven crossover between regions dominated by isotropic liquid and smectic A pretransitional fluctuations. We propose a pioneering minimal model describing plastic crystal phase behavior where we mimic derivation of classical Landau-de Gennes-Ginzburg modeling of Isotropic-Nematic-Smectic A LC phase behavior.
A Global Review on Short Peptides: Frontiers and Perspectives
Authors: Apostolopoulos Vasso , Bojarska Joanna , Chai Tsun-Thai , Elnagdy Sherif , Kaczmarek Krzysztof , Matsoukas John , New Roger , Parang Keykavous , Lopez Octavio Paredes , Parhiz Hamideh , Perera Conrad O. , Pickholz Monica , Remko Milan , Saviano Michele , Skwarczynski Mariusz , Tang Yefeng , Wolf Wojciech M. , Yoshiya Taku , Zabrocki Janusz , Zielenkiewicz Piotr , AlKhazindar Maha , Barriga Vanessa , Kelaidonis Konstantinos , Sarasia Elham Mousavinezhad , Toth Istvan
Published: January 2021

Peptides are fragments of proteins that carry out biological functions. They act as signaling entities via all domains of life and interfere with protein-protein interactions, which are indispensable in bio-processes. Short peptides include fundamental molecular information for a prelude to the symphony of life. They have aroused considerable interest due to their unique features and great promise in innovative bio-therapies. This work focusing on the current state-of-the-art short peptide-based therapeutical developments is the first global review written by researchers from all continents, as a celebration of 100 years of peptide therapeutics since the commencement of insulin therapy in the 1920s. Peptide “drugs” initially played only the role of hormone analogs to balance disorders. Nowadays, they achieve numerous biomedical tasks, can cross membranes, or reach intracellular targets. The role of peptides in bio-processes can hardly be mimicked by other chemical substances. The article is divided into independent sections, which are related to either the progress in short peptide-based theranostics or the problems posing challenge to bio-medicine. In particular, the SWOT analysis of short peptides, their relevance in therapies of diverse diseases, improvements in (bio)synthesis platforms, advanced nano-supramolecular technologies, aptamers, altered peptide ligands and in silico methodologies to overcome peptide limitations, modern smart bio-functional materials, vaccines, and drug/gene-targeted delivery systems are discussed.
HPLC and ToF‒SIMS Analyses of Toxicodendron vernicifluum Tree Sap Mixed with Other Natural Lacquers
Authors: Yu Hye Hyun , Ham Seung Wook , Lee Yeonhee
Published: January 2021

Lacquer sap has been used by humans from antiquitywhen it was treated as a luxury item because of its desirable physical properties. In modern times, although access barriers are lower, lacquer is still considered to be rare and valuable. Thus, low quality, inexpensive Vietnamese and Myanmarese lacquers and cashew nutshell liquid are frequently added to the costly Toxicodendron vernicifluum lacquer sap from Korea, China, and Japan. However, these blended lacquers can diminish the quality of artisan works. The Toxicodendron vernicifluum lacquer saps mixed with other natural lacquers were characterized using time-of-flight secondary-ion mass spectrometry (ToF−SIMS) and high-performance liquid chromatography (HPLC). ToF-SIMS provided the chemical structure of the lacquer monomer, copolymerized dimers, trimers, etc. HPLC provided quantitative analysis of the components of a randomly mixed lacquer. These techniques can be used to control the quality of commercial lacquer sap for the Asian lacquer industry and the traditional conservation of ancient objects.
High-Performance Liquid Chromatography-Tandem Mass Spectrometry for Buprenorphine Evaluation in Plasma—Application to Pharmacokinetic Studies in Rabbits
Authors: Tikhomirov Marta , Poźniak Błażej , Śniegocki Tomasz
Published: January 2021

The precise and reliable determination of buprenorphine concentration is fundamental in certain medical or research applications, particularly in pharmacokinetic studies of this opioid. The main challenge is, however, the development of an analytical method that is sensitive enough, as the detected in vivo concentrations often fall in very low ranges. Thus, in this study we aimed at developing a sensitive, repeatable, cost-efficient, and easy HPLC analytical protocol for buprenorphine in rabbit plasma. In order to obtain this, the HPLC-MS2 system was used to elaborate and validate the method for samples purified with liquid-liquid extraction. Fragment ions 468.6→396.2 and 468.6→414.2 were monitored, and the method resulted in a high repeatability and reproducibility and a limit of quantification of 0.25 µg/L with a recovery of 98.7–109.0%. The method was linear in a range of 0.25–2000 µg/L. The suitability of the analytical procedure was tested in rabbits in a pilot pharmacokinetic study, and it was revealed that the method was suitable for comprehensively describing the pharmacokinetic profile after buprenorphine intravenous administration at a dose of 300 µg/kg. Thus, the method suitability for pharmacokinetic application was confirmed by both the good validation results of the method and successful in vivo tests in rabbits.
Effectiveness of In-Hospital Cholecalciferol Use on Clinical Outcomes in Comorbid COVID-19 Patients: A Hypothesis-Generating Study
Authors: Giannini Sandro , Passeri Giovanni , Tripepi Giovanni , Sella Stefania , Fusaro Maria , Arcidiacono Gaetano , Torres Marco Onofrio , Michielin Alberto , Prandini Tancredi , Baffa Valeria , Aghi Andrea , Egan Colin Gerard , Brigo Martina , Zaninotto Martina , Plebani Mario , Vettor Roberto , Fioretto Paola , Rossini Maurizio , Vignali Alessandro , Fabris Fabrizio , Bertoldo Francesco
Published: January 2021

Little information is available on the beneficial effects of cholecalciferol treatment in comorbid patients hospitalized for COVID-19. The aim of this study was to retrospectively examine the clinical outcome of patients receiving in-hospital high-dose bolus cholecalciferol. Patients with a positive diagnosis of SARS-CoV-2 and overt COVID-19, hospitalized from 15 March to 20 April 2020, were considered. Based on clinical characteristics, they were supplemented (or not) with 400,000 IU bolus oral cholecalciferol (200,000 IU administered in two consecutive days) and the composite outcome (transfer to intensive care unit; ICU and/or death) was recorded. Ninety-one patients (aged 74 ± 13 years) with COVID-19 were included in this retrospective study. Fifty (54.9%) patients presented with two or more comorbid diseases. Based on the decision of the referring physician, 36 (39.6%) patients were treated with vitamin D. Receiver operating characteristic curve analysis revealed a significant predictive power of the four variables: (a) low (<50 nmol/L) 25(OH) vitamin D levels, (b) current cigarette smoking, (c) elevated D-dimer levels (d) and the presence of comorbid diseases, to explain the decision to administer vitamin D (area under the curve = 0.77, 95% CI: 0.67–0.87, p < 0.0001). Over the follow-up period (14 ± 10 days), 27 (29.7%) patients were transferred to the ICU and 22 (24.2%) died (16 prior to ICU and six in ICU). Overall, 43 (47.3%) patients experienced the combined endpoint of transfer to ICU and/or death. Logistic regression analyses revealed that the comorbidity burden significantly modified the effect of vitamin D treatment on the study outcome, both in crude (p = 0.033) and propensity score-adjusted analyses (p = 0.039), so the positive effect of high-dose cholecalciferol on the combined endpoint was significantly amplified with increasing comorbidity burden. This hypothesis-generating study warrants the formal evaluation (i.e., clinical trial) of the potential benefit that cholecalciferol can offer in these comorbid COVID-19 patients.
Association of BMI, Diet, Physical Activity, and Oral Hygiene Practices with DMFT Index of Male Dental Students at King Faisal University, Al-Ahsa
Authors: Jouhar Rizwan , Ahmed Muhammad Adeel , Khurshid Zohaib , Bokhari Syed Akhtar Hussain
Published: January 2021

Despite sufficient knowledge of good oral hygiene habits, dental students still suffer from oral health problems owing to dietary habits, obesity, and a sedentary lifestyle. This cross-sectional study was conducted to evaluate an association of BMI (body mass index), diet, physical activity, and oral hygiene practices with DMFT (decayed, missing, and filled teeth) of male dental students and interns at King Faisal University, Saudi Arabia, from August to September 2020. One hundred and eighty-five male participants completed the study questionnaire. The questionnaire consisted of sociodemographic information and nineteen close-ended questions about current diet, physical activity, and oral hygiene practices. Students’ height (cm) and weight (kg) were measured to calculate BMI. The principal investigator performed the oral clinical examinations for the DMFT status in the dental clinic. The chi-square test was used for the dichotomous variables and a t-test was used for the continuous variables. Linear and multinomial logistic regression were performed to detect the significant predictors of the DMFT score. The mean age of participants was 22.29 ± 2.13 years, and the BMI was 24.94 ± 3.36 (Kg/m2). Parents’ higher education and income levels were significantly (p < 0.001) associated with a higher BMI. Most dietary variables, especially sugar products, and low physical activity, were significantly (<0.047) associated with higher BMI. All oral hygiene practices, except miswak and mouthwash, were significantly (<0.003) associated with higher BMI. Decayed and missing teeth were significantly (<0.001) higher in the overweight and obese. A simple linear regression analysis demonstrated association between BMI and decayed teeth, with an R = 0.35 (<0.001); BMI and missing teeth had an R = 0.12 (p = 0.867); BMI and filled teeth had an R = 0.15 (p = 0.033), and BMI with DMFT had an R = 0.33 ((<0.001). This study demonstrated a strong significant association of the decayed and missing teeth with higher BMI levels. In addition, BMI was significantly associated with diet and physical activity, despite acceptable oral hygiene practices.
Endocrine-Disrupting Organochlorine Pesticides in Human Breast Milk: Changes during Lactation
Authors: Witczak Agata , Pohoryło Anna , Abdel-Gawad Hassan
Published: January 2021

The aim of the present study was to assess infant safety associated with the occurrence of endocrine-disrupting organochlorine pesticides (OCP) in breast milk. Moreover, the association between pregnant mothers’ dietary habits and these compounds levels in breast milk was investigated. Breast milk was collected at various stages of lactation. The samples were analyzed by the GC-MS method. The OCP concentrations ranged from < limit of detection (LOD) to 6.81 ng/g lipids. The highest OCP concentrations in breast milk occurred primarily within the first month of lactation, and decreased over the lactation period. It was found that the maternal consumption of certain food products—in particular pork, beef, poultry, eggs, and dairy products—could have affected the content of 1,1’-(2,2,2-Trichloroethane-1,1-diyl)bis(4-chlorobenzene), called DDT and its metabolites in the breast milk. The levels of beta-endosulfan were positively correlated with fish and poultry consumption. The redundancy analysis indicated that the diets of the pregnant women had an important impact on pesticide residues in the breast milk. There is a potential possibility of lowering the content of organochlorine compounds in breast milk by adhering to nutritional recommendations, e.g., avoiding the excessive consumption of fish and other raw food materials of unknown origin.
Prenatal and Postpartum Maternal Iodide Intake from Diet and Supplements, Urinary Iodine and Thyroid Hormone Concentrations in a Region of the United Kingdom with Mild-to-Moderate Iodine Deficiency
Authors: Threapleton Diane E. , Waiblinger Dagmar , Snart Charles J.P. , Taylor Elizabeth , Keeble Claire , Ashraf Samina , Bi Shazia , Ajjan Ramzi , Azad Rafaq , Hancock Neil , Mason Dan , Reid Stephen , Cromie Kirsten J. , Alwan Nisreen A. , Zimmermann Michael , Stewart Paul M. , Simpson Nigel A.B. , Wright John , Cade Janet E. , Hardie Laura J. , Greenwood Darren C.
Published: January 2021

Iodine is essential for normal thyroid function, supporting healthy fetal and child development. Iodine requirements increase in pregnancy, but many women in regions without salt iodization have insufficient intakes. We explored associations between iodide intake and urinary iodine concentration (UIC), urinary iodine/creatinine ratio (I/Cr), thyroid stimulating hormone, thyroglobulin, free triiodothyronine, free thyroxine and palpable goiter in a region of mild-to-moderate iodine insufficiency. A total of 246 pregnant women aged 18–40 in Bradford, UK, joined the Health and Iodine in Babies (Hiba) study. They provided detailed information on diet and supplement use, urine and serum samples and were assessed for goiter at around 12, 26 and 36 weeks’ gestation, and 6, 18 and 30 weeks postpartum. Dietary iodide intake from food and drink was estimated using six 24 h recalls. During pregnancy, median (IQR) dietary iodide intake was 101 µg/day (54, 142), with 42% from dairy and 9% from white fish. Including supplements, intake was 143 µg/day (94, 196), with 49% < UK reference nutrient intake (140 µg/day). Women with Pakistani heritage had 129 µg/day (87, 190) median total intake. Total intake during pregnancy was associated with 4% (95% CI: 1%, 7%) higher UIC, 5% (3%, 7%) higher I/Cr, 4% (2%, 6%) lower thyroglobulin and 21% (9%, 32%) lower odds of palpable goiter per 50 µg/day. This cohort consumed less iodide in pregnancy than UK and World Health Organization dietary recommendations. UIC, I/Cr and thyroglobulin were associated with intake. Higher intake was associated with fewer goiters. Because dairy was the dominant source of iodide, women following plant-based or low-dairy diets may be at particular risk of iodine insufficiency.
Effects of School-Based Exercise and Nutrition Intervention on Body Composition and Physical Fitness in Overweight Adolescent Girls
Authors: Bogataj Špela , Trajković Nebojša , Cadenas-Sanchez Cristina , Sember Vedrana
Published: January 2021

Regular exercise during school hours is encouraged since childhood obesity has reached epidemic proportions. Moreover, a great majority of adolescents do not meet the recommendations for moderate-to-vigorous physical activity. The present study aimed to determine the effects of school-based high-intensity interval training (HIIT) and nutrition intervention on body composition and physical fitness in overweight adolescent girls. Forty-eight girls were included in the study, of whom 24 (age = 15.5 ± 0.7 years) were randomized to a experimental group (EXP) (HIIT and nutrition intervention school-based program) and 24 (age = 15.7 ± 0.6 years) to a control group (CON) that maintained their usual physical education activities. HIIT consisted of 10 stations of own bodyweight exercise and was done three times per week for eight weeks. Moreover, the EXP participated in the nutrition program led by a nutritionist two times a week. Apart from body composition assessment, participants performed countermovement jump (CMJ), medicine ball throw, hand-grip test, and Yo-Yo Intermittent Recovery Level 1 Test (YYIRT1). A significant effect of group (EXP vs. CON) x time (pre vs. post) interaction was observed for weight [F(1,44) = 7.733; p = 0.008], body mass index [F(1,44) = 5.764; p = 0.020], body fat (in kg) [F(1,44) = 17.850; p < 0.001], and body fat (in %) [F(1,44) = 18.821; p < 0.001]. Moreover, a significant interaction was observed for the medicine ball throw [F(1,44) = 27.016; p < 0.001] and YYIRT1 [F(1,44) = 5.439; p = 0.024]. A significant main effect for time was found for hand grip [F(1,44) = 9.300; p = 0.004] and CMJ [F(1,44) = 12.866; p = 0.001].The present study has demonstrated that just eight weeks school-based HIIT and nutrition intervention, including three sessions a week, can improve body composition and muscular and physical aerobic performance in overweighted adolescent girls.
In Silico Analysis of Possible Interaction between Host Genomic Transcription Factors (TFs) and Zika Virus (ZikaSPH2015) Strain with Combinatorial Gene Regulation; Virus Versus Host—The Game Reloaded
Authors: Chetta Massimiliano , Tarsitano Marina , Vicari Laura , Saracino Annalisa , Bukvic Nenad
Published: January 2021

In silico analysis is a promising approach for understanding biological events in complex diseases. Herein we report on the innovative computational workflow allowed to highlight new direct interactions between human transcription factors (TFs) and an entire genome of virus ZikaSPH2015 strain in order to identify the occurrence of specific motifs on a genomic Zika Virus sequence that is able to bind and, therefore, sequester host’s TFs. The analysis pipeline was performed using different bioinformatics tools available online (free of charge). According to obtained results of this in silico analysis, it is possible to hypothesize that these TFs binding motifs might be able to explain the complex and heterogeneous phenotype presentation in Zika-virus-affected fetuses/newborns, as well as the less severe condition in adults. Moreover, the proposed in silico protocol identified thirty-three different TFs identical to the distribution of TFBSs (Transcription Factor Binding Sites) on ZikaSPH2015 strain, potentially able to influence genes and pathways with biological functions confirming that this approach could find potential answers on disease pathogenesis.
Amorphous Solid Dispersions and the Contribution of Nanoparticles to In Vitro Dissolution and In Vivo Testing: Niclosamide as a Case Study
Authors: Jara Miguel O. , Warnken Zachary N. , Williams Robert O.
Published: January 2021

We developed an amorphous solid dispersion (ASD) of the poorly water-soluble molecule niclosamide that achieved a more than two-fold increase in bioavailability. Notably, this niclosamide ASD formulation increased the apparent drug solubility about 60-fold relative to the crystalline material due to the generation of nanoparticles. Niclosamide is a weakly acidic drug, Biopharmaceutics Classification System (BCS) class II, and a poor glass former with low bioavailability in vivo. Hot-melt extrusion is a high-throughput manufacturing method commonly used in the development of ASDs for increasing the apparent solubility and bioavailability of poorly water-soluble compounds. We utilized the polymer poly(1-vinylpyrrolidone-co-vinyl acetate) (PVP–VA) to manufacture niclosamide ASDs by extrusion. Samples were analyzed based on their microscopic and macroscopic behavior and their intermolecular interactions, using differential scanning calorimetry (DSC), X-ray diffraction (XRD), nuclear magnetic resonance (NMR), Fourier-transform infrared (FTIR), and dynamic light scattering (DLS). The niclosamide ASD generated nanoparticles with a mean particle size of about 100 nm in FaSSIF media. In a side-by-side diffusion test, these nanoparticles produced a four-fold increase in niclosamide diffusion. We successfully manufactured amorphous extrudates of the poor glass former niclosamide that showed remarkable in vitro dissolution and diffusion performance. These in vitro tests were translated to a rat model that also showed an increase in oral bioavailability.
Effect of Lead and Copper on Photosynthetic Apparatus in Citrus (Citrus aurantium L.) Plants. The Role of Antioxidants in Oxidative Damage as a Response to Heavy Metal Stress
Authors: Giannakoula Anastasia , Therios Ioannis , Chatzissavvidis Christos
Published: January 2021

Photosynthetic changes and antioxidant activity to oxidative stress were evaluated in sour orange (Citrus aurantium L.) leaves subjected to lead (Pb), copper (Cu) and also Pb + Cu toxicity treatments, in order to elucidate the mechanisms involved in heavy metal tolerance. The simultaneous effect of Pb− and Cu on growth, concentration of malondialdehyde (MDA), hydrogen peroxide (H2O2), chlorophylls, flavonoids, carotenoids, phenolics, chlorophyll fluorescence and photosynthetic parameters were examined in leaves of Citrus aurantium L. plants. Exogenous application of Pb and Cu resulted in an increase in leaf H2O2 and lipid peroxidation (MDA). Toxicity symptoms of both Pb and Cu treated plants were stunted growth and decreased pigments concentration. Furthermore, photosynthetic activity of treated plants exhibited a significant decline. The inhibition of growth in Pb and Cu-treated plants was accompanied by oxidative stress, as indicated by the enhanced lipid peroxidation and the high H2O2 concentration. Furthermore, antioxidants in citrus plants after exposure to high Pb and Cu concentrations were significantly increased compared to control and low Pb and Cu treatments. In conclusion, this study indicates that Pb and Cu promote lipid peroxidation, disrupt membrane integrity, reduces growth and photosynthesis and inhibit mineral nutrition. Considering the potential for adverse human health effects associated with high concentrations of Pb and Cu contained in edible parts of citrus plants the study signals that it is important to conduct further research into the accessibility and uptake of the tested heavy metals in the soil and whether they pose risks to humans.
Synthesis and Application of Arylaminophosphazene as a Flame Retardant and Catalyst for the Polymerization of Benzoxazines
Authors: Bornosuz Natalia V. , Gorbunova Irina Yu. , Kireev Vyacheslav V. , Bilichenko Yulya V. , Chursova Larisa V. , Svistunov Yuri S. , Onuchin Denis V. , Shutov Vyacheslav V. , Petrakova Viktoria V. , Kolenchenko Alexander A. , Nguyen Duong T. , Pavlov Nikolay V. , Orlov Alexey V. , Grebeneva Tatyana A. , Sirotin Igor S.
Published: January 2021

A novel type of phosphazene containing an additive that acts both as a catalyst and as a flame retardant for benzoxazine binders is presented in this study. The synthesis of a derivative of hexachlorocyclotriphosphazene (HCP) and meta-toluidine was carried out in the medium of the latter, which made it possible to achieve the complete substitution of chlorine atoms in the initial HCP. Thermal and flammability characteristics of modified compositions were investigated. The modifier catalyzes the process of curing and shifts the beginning of reaction from 222.0 °C for pure benzoxazine to 205.9 °C for composition with 10 phr of modifier. The additive decreases the glass transition temperature of compositions. Achievement of the highest category of flame resistance (V-0 in accordance with UL-94) is ensured both by increasing the content of phenyl residues in the composition and by the synergistic effect of phosphorus and nitrogen. A brief study of the curing kinetics disclosed the complex nature of the reaction. An accurate two-step model is obtained using the extended Prout–Tompkins equation for both steps.
Additive-Manufactured Gyroid Scaffolds of Magnesium Oxide, Phosphate Glass Fiber and Polylactic Acid Composite for Bone Tissue Engineering
Authors: He Lizhe , Liu Xiaoling , Rudd Chris
Published: January 2021

Composites of biodegradable phosphate glass fiber and polylactic acid (PGF/PLA) show potential for bone tissue engineering scaffolds, due to their ability to release Ca, P, and Mg during degradation, thus promoting the bone repair. Nevertheless, glass degradation tends to acidify the surrounding aqueous environment, which may adversely affect the viability and bone-forming activities of osteoblasts. In this work, MgO was investigated as a neutralizing agent. Porous network-phase gyroid scaffolds were additive-manufactured using four different materials: PLA, MgO/PLA, PGF/PLA, and (MgO + PGF)/PLA. The addition of PGF enhanced compressive properties of scaffolds, and the resultant scaffolds were comparably strong and stiff with human trabecular bone. While the degradation of PGF/PLA composite induced considerable acidity in degradation media and intensified the degradation of PGF in return, the degradation media of (MgO + PGF)/PLA maintained a neutral pH close to a physiological environment. The experiment results indicated the possible mechanism of MgO as the neutralizing agent: the local acidity was buffered as the MgO reacted with the acidic degradation products thereby inhibiting the degradation of PGF from being intensified in an acidic environment. The (MgO + PGF)/PLA composite scaffold appears to be a candidate for bone tissue engineering.
Integrated Drought Monitoring and Evaluation through Multi-Sensor Satellite-Based Statistical Simulation
Authors: Kim Jong-Suk , Park Seo-Yeon , Lee Joo-Heon , Chen Jie , Chen Si , Kim Tae-Woong
Published: January 2021

To proactively respond to changes in droughts, technologies are needed to properly diagnose and predict the magnitude of droughts. Drought monitoring using satellite data is essential when local hydrogeological information is not available. The characteristics of meteorological, agricultural, and hydrological droughts can be monitored with an accurate spatial resolution. In this study, a remote sensing-based integrated drought index was extracted from 849 sub-basins in Korea’s five major river basins using multi-sensor collaborative approaches and multivariate dimensional reduction models that were calculated using monthly satellite data from 2001 to 2019. Droughts that occurred in 2001 and 2014, which are representative years of severe drought since the 2000s, were evaluated using the integrated drought index. The Bayesian principal component analysis (BPCA)-based integrated drought index proposed in this study was analyzed to reflect the timing, severity, and evolutionary pattern of meteorological, agricultural, and hydrological droughts, thereby enabling a comprehensive delivery of drought information.
Power-over-Fiber LPIT for Voltage and Current Measurements in the Medium Voltage Distribution Networks
Authors: Bassan Fabio R. , Rosolem Joao B. , Floridia Claudio , Aires Bruno N. , Peres Rodrigo , Aprea Javier F. , Nascimento Carlos Alexandre M. , Fruett Fabiano
Published: January 2021

In this work, we present the design, laboratory tests, and the field trial results of a power-over-fiber (PoF) low power instrument transformer (LPIT) for voltage and current measurements in the medium voltage distribution networks. The new proposed design of this power-over-fiber LPIT aims to overcome the drawbacks presented by the previous technologies, such as the continuous operation (measuring and data transmission) for a wide current range conducted in the medium voltage transmission lines, damage due to lightning strikes, accuracy dependency on vibration, position and temperatures. The LPIT attends the accuracy criteria of IEC 61869-10 and IEC 61869-11 in terms of current and voltage accuracy and it attends the practical criteria adopted by Utilities companies including voltage measurements without removing the coating of the covered conductors. The PoF based LPIT was developed to be applied at 11.9 kV, 13.8 kV, and 23.0 kV phase-to-phase nominal voltages, and in two current ranges 1.25–30 A and 37.5–900 A. The digital data transmission of current, voltage, and temperature from the sensing unit to the processing unit uses a special synchronism technique and it is performed by two 62.5 µm multimode fibers in 850 nm. The optical powering in 976 nm is also performed by one 62.5 µm multimode fiber from the processing unit to the sensor unit. We presented all details of the sensor design and its laboratory characterization in terms of accuracy and temperature correction. We also presented the results of field tests of the sensor made in two different conditions: in a standard distribution network and an experimental hybrid fiber/power distribution network. We believe that these studies aim to incorporate optical fiber and devices, digital technologies, communications systems in electrical systems driving their evolution.
Automated Sensing System for Real-Time Recognition of Trucks in River Dredging Areas Using Computer Vision and Convolutional Deep Learning
Authors: Chou Jui-Sheng , Liu Chia-Hsuan
Published: January 2021

Sand theft or illegal mining in river dredging areas has been a problem in recent decades. For this reason, increasing the use of artificial intelligence in dredging areas, building automated monitoring systems, and reducing human involvement can effectively deter crime and lighten the workload of security guards. In this investigation, a smart dredging construction site system was developed using automated techniques that were arranged to be suitable to various areas. The aim in the initial period of the smart dredging construction was to automate the audit work at the control point, which manages trucks in river dredging areas. Images of dump trucks entering the control point were captured using monitoring equipment in the construction area. The obtained images and the deep learning technique, YOLOv3, were used to detect the positions of the vehicle license plates. Framed images of the vehicle license plates were captured and were used as input in an image classification model, C-CNN-L3, to identify the number of characters on the license plate. Based on the classification results, the images of the vehicle license plates were transmitted to a text recognition model, R-CNN-L3, that corresponded to the characters of the license plate. Finally, the models of each stage were integrated into a real-time truck license plate recognition (TLPR) system; the single character recognition rate was 97.59%, the overall recognition rate was 93.73%, and the speed was 0.3271 s/image. The TLPR system reduces the labor force and time spent to identify the license plates, effectively reducing the probability of crime and increasing the transparency, automation, and efficiency of the frontline personnel’s work. The TLPR is the first step toward an automated operation to manage trucks at the control point. The subsequent and ongoing development of system functions can advance dredging operations toward the goal of being a smart construction site. By intending to facilitate an intelligent and highly efficient management system of dredging-related departments by providing a vehicle LPR system, this paper forms a contribution to the current body of knowledge in the sense that it presents an objective approach for the TLPR system.
Hyper-Angle Exploitative Searching for Enabling Multi-Objective Optimization of Fog Computing
Authors: Naser Abdali Taj-Aldeen , Hassan Rosilah , Mohd Aman Azana Hafizah , Nguyen Quang Ngoc , Al-Khaleefa Ahmed Salih
Published: January 2021

Fog computing is an emerging technology. It has the potential of enabling various wireless networks to offer computational services based on certain requirements given by the user. Typically, the users give their computing tasks to the network manager that has the responsibility of allocating needed fog nodes optimally for conducting the computation effectively. The optimal allocation of nodes with respect to various metrics is essential for fast execution and stable, energy-efficient, balanced, and cost-effective allocation. This article aims to optimize multiple objectives using fog computing by developing multi-objective optimization with high exploitive searching. The developed algorithm is an evolutionary genetic type designated as Hyper Angle Exploitative Searching (HAES). It uses hyper angle along with crowding distance for prioritizing solutions within the same rank and selecting the highest priority solutions. The approach was evaluated on multi-objective mathematical problems and its superiority was revealed by comparing its performance with benchmark approaches. A framework of multi-criteria optimization for fog computing was proposed, the Fog Computing Closed Loop Model (FCCL). Results have shown that HAES outperforms other relevant benchmarks in terms of non-domination and optimality metrics with over 70% confidence of the t-test for rejecting the null-hypothesis of non-superiority in terms of the domination metric set coverage.
Relationship between Muscular Activity and Postural Control Changes after Proprioceptive Focal Stimulation (Equistasi®) in Middle-Moderate Parkinson’s Disease Patients: An Explorative Study
Authors: Spolaor Fabiola , Romanato Marco , Annamaria Guiotto , Peppe Antonella , Bakdounes Leila , To Duc-Khanh , Volpe Daniele , Sawacha Zimi
Published: January 2021

The aim of this study was to investigate the effects of Equistasi®, a wearable device, on the relationship between muscular activity and postural control changes in a sample of 25 Parkinson’s disease (PD) subjects. Gait analysis was carried out through a six-cameras stereophotogrammetric system synchronized with two force plates, an eight-channel surface electromyographic system, recording the activity of four muscles bilaterally: Rectus femoris, tibialis anterior (TA), biceps femoris, and gastrocnemius lateralis (GL). The peak of the envelope (PoE) and its occurrence within the gait cycle (position of the peak of the envelope, PPoE) were calculated. Frequency-domain posturographic parameters were extracted while standing still on a force plate in eyes open and closed conditions for 60 s. After the treatment with Equistasi®, the mid-low (0.5–0.75) Hz and mid-high (0.75–1 Hz) components associated with the vestibular and somatosensory systems, PoE and PPoE, displayed a shift toward the values registered on the controls. Furthermore, a correlation was found between changes in proprioception (power spectrum frequencies during the Romberg Test) and the activity of GL, BF (PoE), and TA (PPoE). Results of this study could provide a quantitative estimation of the effects of a neurorehabilitation device on the peripheral and central nervous system in PD.
Quantitative Evaluations with 2d Electrical Resistance Tomography in the Low-Conductivity Solutions Using 3d-Printed Phantoms and Sucrose Crystal Agglomerate Assessments
Authors: Rao Guruprasad , Sattar Muhammad Awais , Wajman Radosław , Jackowska-Strumiłło Lidia
Published: January 2021

Crystallization is a significant procedure in the manufacturing of many pharmaceutical and solid food products. In-situ electrical resistance tomography (ERT) is a novel process analytical tool (PAT) to provide a cheap and quick way to test, visualize, and evaluate the progress of crystallization processes. In this work, the spatial accuracy of the nonconductive phantoms in low-conductivity solutions was evaluated. Gauss–Newton, linear back projection, and iterative total variation reconstruction algorithms were used to compare the phantom reconstructions for tap water, industrial-grade saturated sucrose solution, and demineralized water. A cylindrical phantom measuring 10 mm in diameter and a cross-section area of 1.5% of the total beaker area was detected at the center of the beaker. Two phantoms with a 10-mm diameter were visualized separately in noncentral locations. The quantitative evaluations were done for the phantoms with radii ranging from 10 mm to 50 mm in demineralized water. Multiple factors, such as ERT device and sensor development, Finite Element Model (FEM) mesh density and simulations, image reconstruction algorithms, number of iterations, segmentation methods, and morphological image processing methods, were discussed and analyzed to achieve spatial accuracy. The development of ERT imaging modality for the purpose of monitoring crystallization in low-conductivity solutions was performed satisfactorily.
Evaluation of Hygrothermal Behaviour in Heritage Buildings through Sensors, CFD Modelling and IRT
Authors: Lerma Carlos , Borràs Júlia G. , Mas Ángeles , Torner M. Eugenia , Vercher Jose , Gil Enrique
Published: January 2021

Architectural heritage, building materials and interior space are highly susceptible to temperature and relative humidity. A better knowledge of the hygrothermal dynamics inside buildings allows an adequate conservation of heritage. This work compares three non-destructive techniques (NDT), such as temperature and relative humidity sensors, finite element simulations (CFD) and thermographic pictures (IRT). The work has made it possible to carry out an assessment of the risk of condensation over a year and to identify affected periods and areas of the building. Sensors and IRT pictures provide real data to validate CFD simulations, facilitating a global analysis of the building. The results provided reflect a great concordance between the NDTs used.