bluuebunny/crossref_metadata_2025 huggingface dataset:
Multifunctional Lipid-Based Nanoparticles for Codelivery of Anticancer Drugs and siRNA for Treatment of Non-Small Cell Lung Cancer with Different Level of Resistance and EGFR Mutations
Authors: Majumder Joydeb , Minko Tamara
Published: July 2021

Resistance to chemotherapy, enhanced proliferation, invasion, angiogenesis, and metastasis (RPIAM) represent major obstacles that limit the efficacy of cancer treatment especially in advanced stages of cancer. Overcoming or suppressing RPIAM can dramatically improve the treatment outcome. Non-small cell lung cancer (NSCLC) is frequently diagnosed in an advanced stage and often possesses intrinsic resistance to chemotherapy accompanied by the fast development of acquired resistance during the treatment. Oncogenic receptor tyrosine kinases (TKs), specifically epidermal growth factor (EGF) TKs, play an important role in the activation of MAPK/PI3K/Akt/STAT pathways, finally leading to the development of RPIAM. However, the suppression of EGF-TK by different drugs is limited by various defensive mechanisms and mutations. In order to effectively prevent the development of RPIAM in NSCLC, we formulated and tested a multicomponent and multifunctional cancer targeted delivery system containing Nanostructured Lipid Carriers (NLCs) as vehicles, luteinizing hormone release hormone (LHRH) as a cancer targeting moiety, EFG-TK inhibitor gefitinib and/or paclitaxel as anticancer drug(s), siRNA targeted to EGF receptor (EGFR) mRNA as a suppressor of EGF receptors, and an imaging agent (rhodamine) for the visualization of cancer cells. Experimental data obtained show that this complex delivery system possesses significantly enhanced anticancer activity that cannot be achieved by individual components applied separately.
Therapeutic Development Based on the Immunopathogenic Mechanisms of Psoriasis
Authors: Tseng Jen-Chih , Chang Yung-Chi , Huang Chun-Ming , Hsu Li-Chung , Chuang Tsung-Hsien
Published: July 2021

Psoriasis, a complex inflammatory autoimmune skin disorder that affects 2–3% of the global population, is thought to be genetically predetermined and induced by environmental and immunological factors. In the past decades, basic and clinical studies have significantly expanded knowledge on the molecular, cellular, and immunological mechanisms underlying the pathogenesis of psoriasis. Based on these pathogenic mechanisms, the current disease model emphasizes the role of aberrant Th1 and Th17 responses. Th1 and Th17 immune responses are regulated by a complex network of different cytokines, including TNF-α, IL-17, and IL-23; signal transduction pathways downstream to the cytokine receptors; and various activated transcription factors, including NF-κB, interferon regulatory factors (IRFs), and signal transducer and activator of transcriptions (STATs). The biologics developed to specifically target the cytokines have achieved a better efficacy and safety for the systemic management of psoriasis compared with traditional treatments. Nevertheless, the current therapeutics can only alleviate the symptoms; there is still no cure for psoriasis. Therefore, the development of more effective, safe, and affordable therapeutics for psoriasis is important. In this review, we discussed the current trend of therapeutic development for psoriasis based on the recent discoveries in the immune modulation of the inflammatory response in psoriasis.
Stability-Indicating Analytical Approach for Stability Evaluation of Lactoferrin
Authors: Osel Nika , Planinšek Parfant Timeja , Kristl Albin , Roškar Robert
Published: July 2021

Lactoferrin is a multifunctional iron-binding glycoprotein in milk. Due to its potential for the treatment of various diseases, interest in products containing lactoferrin is increasing. However, as a protein, it is prone to degradation, which critically affects the quality of products. Therefore, the main purpose of our work was to develop a stability-indicating analytical approach for stability evaluation of lactoferrin. We were focused on two complementary methods: reversed-phase and size-exclusion chromatography. The stability-indicating nature of the selected methods was confirmed. They were successfully validated by following the ICH guidelines and applied to preliminary lactoferrin stability studies. Up to three degradation products, as well as aggregates and fragments of lactoferrin, were detected in various samples using complementary reversed-phase and size-exclusion chromatographic methods. The analytical approach was additionally extended with three spectroscopic techniques (absorbance, intrinsic fluorescence, and bicinchoninic acid method), which may provide valuable complementary information in some cases. The presented analytical approach allows the stability evaluation of lactoferrin in various samples, including the ability to detect differences in its degradation mechanisms. Furthermore, it has the potential to be used for the quality control of products containing lactoferrin.
Deformation Behavior and Fracture Patterns of Laminated PEEK- and PI-Based Composites with Various Carbon-Fiber Reinforcement
Authors: Kosmachev Pavel V , Alexenko Vladislav O , Bochkareva Svetlana A , Panin Sergey V
Published: July 2021

Laminated composites based on polyetheretherketone (PEEK) and polyimide (PI) matrices were fabricated by hot compression. Reinforcing materials (unidirectional carbon-fiber (CF) tapes or carbon fabric) and their layout patterns were varied. Stress–strain diagrams after three-point flexural tests were analyzed, and both lateral faces of the fractured specimens and fractured surfaces (obtained by optical and scanning electron microscopy, respectively) were studied. It was shown that the laminated composites possessed the maximum mechanical properties (flexural elastic modulus and strength) in the case of the unidirectional CF (0°/0°) layout. These composites were also not subjected to catastrophic failure during the tests. The PEEK-based composites showed twice the flexural strength of the PI-based ones (0.4 and 0.2 GPa, respectively), while the flexural modulus was four times higher (60 and 15 GPa, correspondently). The reason was associated with different melt flowability of the used polymer matrices and varied inter- (intra)layer adhesion levels. The effect of adhesion was additionally studied by computer simulation using a developed two-dimensional FE-model. It considered initial defects between the binder and CF, as well as subsequent delamination and failure under loads. Based on the developed FE-model, the influence of defects and delamination on the strength properties of the composites was shown at different stress states, and the corresponding quantitative estimates were reported. Moreover, another model was developed to determine the three-point flexural properties of the composites reinforced with CF and carbon fabric, taking into account different fiber layouts. It was shown within this model framework that the flexural strength of the studied composites could be increased by an order of magnitude by enhancing the adhesion level (considered through the contact area between CF and the binder).
Contribution to a Circular Economy Model: From Lignocellulosic Wastes from the Extraction of Vegetable Oils to the Development of a New Composite
Authors: Dominguez-Candela Ivan , Garcia-Garcia Daniel , Perez-Nakai Aina , Lerma-Canto Alejandro , Lora Jaime , Fombuena Vicent
Published: July 2021

The present works focuses on the development of a novel fully bio-based composite using a bio-based high-density polyethylene (Bio-HDPE) obtained from sugar cane as matrix and a by-product of extraction of chia seed oil (CO) as filler, with the objective of achieving a circular economy model. The research aims to revalorize an ever-increasing waste stream produced by the growing interest in vegetable oils. From the technical point of view, the chia seed flour (CSF) was chemically modified using a silane treatment. This treatment provides a better interfacial adhesion as was evidenced by the mechanical and thermal properties as well as field emission scanning electron microscopy (FESEM). The effect of silane treatment on water uptake and disintegration rate was also studied. On the other hand, in a second stage, an optimization of the percentage of treated CSF used as filler was carried out by a complete series of mechanical, thermal, morphological, colour, water absorption and disintegration tests with the aim to evaluate the new composite developed using chia by-products. It is noteworthy as the disintegration rate increased with the addition of CSF filler, which leads to obtain a partially biodegradable wood plastic composite (WPC) and therefore, becoming more environmentally friendly.
Blends of Semiflexible Polymers: Interplay of Nematic Order and Phase Separation
Authors: Milchev Andrey , Egorov Sergei A. , Midya Jiarul , Binder Kurt , Nikoubashman Arash
Published: July 2021

Mixtures of semiflexible polymers with a mismatch in either their persistence lengths or their contour lengths are studied by Density Functional Theory and Molecular Dynamics simulation. Considering lyotropic solutions under good solvent conditions, the mole fraction and pressure is systematically varied for several cases of bending stiffness κ (the normalized persistence length) and chain length N. For binary mixtures with different chain length (i.e., NA=16, NB=32 or 64) but the same stiffness, isotropic-nematic phase coexistence is studied. For mixtures with the same chain length (N=32) and large stiffness disparity (κB/κA=4.9 to 8), both isotropic-nematic and nematic-nematic unmixing occur. It is found that the phase diagrams may exhibit a triple point or a nematic-nematic critical point, and that coexisting phases differ appreciably in their monomer densities. The properties of the two types of chains (nematic order parameters, chain radii, etc.) in the various phases are studied in detail, and predictions on the (anisotropic) critical behavior near the critical point of nematic-nematic unmixing are made.
Design of Olmesartan Medoxomil-Loaded Nanosponges for Hypertension and Lung Cancer Treatments
Authors: Almutairy Bjad K. , Alshetaili Abdullah , Alali Amer S. , Ahmed Mohammed Muqtader , Anwer Md. Khalid , Aboudzadeh M. Ali
Published: July 2021

Olmesartan medoxomil (OLM) is one of the prominent antihypertensive drug that suffers from low aqueous solubility and dissolution rate leading to its low bioavailability. To improve the oral bioavailability of OLM, a delivery system based on ethylcellulose (EC, a biobased polymer) nanosponges (NSs) was developed and evaluated for cytotoxicity against the A549 lung cell lines and antihypertensive potential in a rat model. Four OLM-loaded NSs (ONS1-ONS4) were prepared and fully evaluated in terms of physicochemical properties. Among these formulations, ONS4 was regarded as the optimized formulation with particle size (487 nm), PDI (0.386), zeta potential (ζP = −18.1 mV), entrapment efficiency (EE = 91.2%) and drug loading (DL = 0.88%). In addition, a nanosized porous morphology was detected for this optimized system with NS surface area of about 63.512 m2/g, pore volume and pore radius Dv(r) of 0.149 cc/g and 15.274 Å, respectively, measured by nitrogen adsorption/desorption analysis. The observed morphology plus sustained release rate of OLM caused that the optimized formulation showed higher cytotoxicity against A549 lung cell lines in comparison to the pure OLM. Finally, this system (ONS4) reduced the systolic blood pressure (SBP) significantly (p < 0.01) as compared to control and pure OLM drug in spontaneously hypertensive rats. Overall, this study provides a scientific basis for future studies on the encapsulation efficiency of NSs as promising drug carriers for overcoming pharmacokinetic limitations.
Calcein Binding to Assess Mineralization in Hydrogel Microspheres
Authors: White Kristopher , Chalaby Rabab , Lowe Gina , Berlin Jacob , Glackin Carlotta , Olabisi Ronke
Published: July 2021

We describe a method to assess mineralization by osteoblasts within microspheres using calcein. Fluorescence imaging of calcein bound to the calcium in hydroxyapatite permits assessment of the mineralized portion of the extracellular matrix. Colorimetric imaging of Alizarin Red S complexed with calcium also gives measures of mineralization, and in tissue cultures calcein and Alizarin Red S have been shown to bind to the same regions of mineral deposits. We show that when the mineralization takes place within hydrogel microspheres, Alizarin Red S does not stain mineral deposits as consistently as calcein. As tissue engineers increasingly encapsulate osteoprogenitors within hydrogel scaffolds, calcein staining may prove a more reliable method to assess this mineralization.
Diversity and Evolution of Clostridium beijerinckii and Complete Genome of the Type Strain DSM 791T
Authors: Sedlar Karel , Nykrynova Marketa , Bezdicek Matej , Branska Barbora , Lengerova Martina , Patakova Petra , Skutkova Helena
Published: July 2021

Clostridium beijerinckii is a relatively widely studied, yet non-model, bacterium. While 246 genome assemblies of its various strains are available currently, the diversity of the whole species has not been studied, and it has only been analyzed in part for a missing genome of the type strain. Here, we sequenced and assembled the complete genome of the type strain Clostridium beijerinckii DSM 791T, composed of a circular chromosome and a circular megaplasmid, and used it for a comparison with other genomes to evaluate diversity and capture the evolution of the whole species. We found that strains WB53 and HUN142 were misidentified and did not belong to the Clostridium beijerinckii species. Additionally, we filtered possibly misassembled genomes, and we used the remaining 237 high-quality genomes to define the pangenome of the whole species. By its functional annotation, we showed that the core genome contains genes responsible for basic metabolism, while the accessory genome has genes affecting final phenotype that may vary among different strains. We used the core genome to reconstruct the phylogeny of the species and showed its great diversity, which complicates the identification of particular strains, yet hides possibilities to reveal hitherto unreported phenotypic features and processes utilizable in biotechnology.
The Use of a Genetic Algorithm for Sorting Warehouse Optimisation
Authors: Grznár Patrik , Krajčovič Martin , Gola Arkadiusz , Dulina Ľuboslav , Furmannová Beáta , Mozol Štefan , Plinta Dariusz , Burganová Natália , Danilczuk Wojciech , Svitek Radovan
Published: July 2021

In the last decade, simulation software as a tool for managing and controlling business processes has received a lot of attention. Many of the new software features allow businesses to achieve better quality results using optimisation, such as genetic algorithms. This article describes the use of modelling and simulation in shipment and sorting processes that are optimised by a genetic algorithm’s involvement. The designed algorithm and simulation model focuses on optimising the duration of shipment processing times and numbers of workers. The commercially available software Tecnomatix Plant Simulation, paired with a genetic algorithm, was used for optimisation, decreasing time durations, and thus selecting the most suitable solution for defined inputs. This method has produced better results in comparison to the classical heuristic methods and, furthermore, is not as time consuming. This article, at its core, describes the algorithm used to determine the optimal number of workers in sorting warehouses with the results of its application. The final part of this article contains an evaluation of this proposal compared to the original methods, and highlights what benefits result from such changes. The major purpose of this research is to determine the number of workers needed to speed up the departure of shipments and optimise the workload of workers.
The “Christology” of Bely the Anthroposophist: Andrei Bely, Rudolf Steiner, and the Apostle Paul
Authors: Spivak Monika
Published: July 2021

The article focuses on R. Steiner’s perception of the Gospels and the impact of that view on Bely’s works. The latter had always valued Steiner’s lectures on Christ and the Fifth Gospel, the “Anthroposophic” (relating to the philosophy of human genesis, existence, and outcome) Gospel, the knowledge of which had been received in a visionary way. In addition, Bely was an esoteric follower of Steiner and often quoted from Apostle Paul’s 2 Corinthians, “Ye are our epistle written in our hearts, known and read of all men”. The citation occurs in Bely’s philosophical works (The History of the Formation of the Self-Conscious Soul, “Crisis of Consciousness”), autobiographic prose (Reminiscences of Steiner), the essay “Why I Became a Symbolist…”, and letters (to Ivanov-Razumnik and Fedor Gladkov). Bely’s own anthroposophic and esoteric ideas relating to the gospel sayings are also examined. The aim of the research is to show through the example of one quotation the specifics of Bely the Anthroposophist’s perception of Christian texts in general. This provides a methodological meaning for understanding other Biblical quotations and images in the works of Bely because anthroposophical Christology is also the key to their deciphering.
Biases for Evil and Moral Perfection
Authors: Van Eyghen Hans
Published: July 2021

I argue that deeply ingrained dispositions to do evil do not render moral perfection impossible. I discuss various accounts of moral perfection and the evidence from cognitive (neuro)science that points towards a strong disposition for evil. Afterwards, I discuss three strategies that can allow humans to overcome their evil dispositions. These are: cognitive enhancement, avoiding triggering situations and structural solutions.
A LiDAR/Visual SLAM Backend with Loop Closure Detection and Graph Optimization
Authors: Chen Shoubin , Zhou Baoding , Jiang Changhui , Xue Weixing , Li Qingquan
Published: July 2021

LiDAR (light detection and ranging), as an active sensor, is investigated in the simultaneous localization and mapping (SLAM) system. Typically, a LiDAR SLAM system consists of front-end odometry and back-end optimization modules. Loop closure detection and pose graph optimization are the key factors determining the performance of the LiDAR SLAM system. However, the LiDAR works at a single wavelength (905 nm), and few textures or visual features are extracted, which restricts the performance of point clouds matching based loop closure detection and graph optimization. With the aim of improving LiDAR SLAM performance, in this paper, we proposed a LiDAR and visual SLAM backend, which utilizes LiDAR geometry features and visual features to accomplish loop closure detection. Firstly, the bag of word (BoW) model, describing the visual similarities, was constructed to assist in the loop closure detection and, secondly, point clouds re-matching was conducted to verify the loop closure detection and accomplish graph optimization. Experiments with different datasets were carried out for assessing the proposed method, and the results demonstrated that the inclusion of the visual features effectively helped with the loop closure detection and improved LiDAR SLAM performance. In addition, the source code, which is open source, is available for download once you contact the corresponding author.
An Improved Cloud Masking Method for GOCI Data over Turbid Coastal Waters
Authors: Lu Shiming , He Mingjun , He Shuangyan , He Shuo , Pan Yunhe , Yin Wenbin , Li Peiliang
Published: July 2021

Clouds severely hinder the radiative transmission of visible light; thus, correctly masking cloudy and non-cloudy pixels is a preliminary step in processing ocean color remote sensing data. However, cloud masking over turbid waters is prone to misjudgment, leading to loss of non-cloudy pixel data. This research proposes an improved cloud masking method over turbid water to classify cloudy and non-cloudy pixels based on spectral variability of Rayleigh-corrected reflectance acquired by the Geostationary Ocean Color Imager (GOCI). Compared with other existing cloud masking methods, we demonstrated that this improved method can identify the spatial positions and shapes of clouds more realistically, and more accurate pixels of turbid waters were retained. This improved method can be effectively applied in typical turbid coastal waters. It has potential to be used in cloud masking procedures of spaceborne ocean color sensors without short-wave infrared bands.
Prototype Calibration with Feature Generation for Few-Shot Remote Sensing Image Scene Classification
Authors: Zeng Qingjie , Geng Jie , Huang Kai , Jiang Wen , Guo Jun
Published: July 2021

Few-shot classification of remote sensing images has attracted attention due to its important applications in various fields. The major challenge in few-shot remote sensing image scene classification is that limited labeled samples can be utilized for training. This may lead to the deviation of prototype feature expression, and thus the classification performance will be impacted. To solve these issues, a prototype calibration with a feature-generating model is proposed for few-shot remote sensing image scene classification. In the proposed framework, a feature encoder with self-attention is developed to reduce the influence of irrelevant information. Then, the feature-generating module is utilized to expand the support set of the testing set based on prototypes of the training set, and prototype calibration is proposed to optimize features of support images that can enhance the representativeness of each category features. Experiments on NWPU-RESISC45 and WHU-RS19 datasets demonstrate that the proposed method can yield superior classification accuracies for few-shot remote sensing image scene classification.
Perceived Changes in Sexuality during the COVID-19 Pandemic among Adults in the United States
Authors: Bowling Jessamyn , Montanaro Erika , Guerrero-Ordonez Sarai , Joshi Stuti , Gioia Diana
Published: July 2021

In the United States, the COVID-19 pandemic has decreased partnered sexual behavior and increased the use of enhancement (e.g., toys). This has been partly attributed to reduced social interactions and stress. However, individuals’ perceptions of changes are missing in research. This study aims to examine how adults perceive changes in their sexuality during the pandemic. We conducted a nationwide survey of US adults from April–June 2020 (N = 326). This qualitative study examines the open-ended responses using thematic analyses. The following themes emerged from the data: (1) changes in the purpose of sex; (2) changes in sexual identity; (3) decreases in sex drive and desire; (4) increases in sex drive and desire; (5) fluctuations in sex drive and desire; (6) increased sexual experimentation and reflection. The stress, changes in home responsibilities and living situations, and time spent with partners (more or less) has affected individuals by increasing or decreasing their sex drive and desire. Participants responded to changes with self-reflection and awareness, and incorporating new practices (e.g., technology, kink). The purpose of sex has shifted in order to gain intimacy or connect, or to pass time. These changes were perceived as both positive and negative, and more research is needed to determine the durability of these changes.
Sustainability Understanding and Behaviors across Urban Areas: A Case Study on Istanbul City
Authors: Topal Hasan Fehmi , Hunt Dexter V. L. , Rogers Christopher D. F.
Published: July 2021

The success of urban sustainability is very much dependent on a number of human factors. Therefore, it becomes even more important to explore how people understand urban sustainability and how they behave accordingly. Based on a formerly developed conceptual framework and on specified influencing factors, this study aimed to evaluate and elucidate the urban sustainability understanding and behavior of individuals in the city of Istanbul. This was assessed through the use of a quantitative questionnaire survey of 535 respondents. Therein, socio-psychological processes of sustainability understanding (i.e., determinants of awareness, perception, and attitude) and sustainability behaviors along with personality traits and influential factors were assessed and analyzed through the use of bivariate and multivariate methods (i.e., correlation tests, ANOVA, t-tests, and multiple linear regression). The results showed that sustainability awareness was more strongly correlated with attitude than perception, whereas behavior was found to be strongly correlated with both awareness and attitude and was (significantly) predicted by all determinants. The associations/influences of personality traits with determinants were found to be mostly insignificant. Conversely, for behavior, they were significant. The most influential factors found (in hierarchical ordering) were awareness of consequences, trust in society, social appraisement, world-mindedness, willingness to pay, trust in science and technology, ascription of responsibility, age and gender.
Study on Coupled Relationship between Urban Air Quality and Land Use in Lanzhou, China
Authors: Yan Cuixia , Wang Lucang , Zhang Qing
Published: July 2021

The intensification of global urbanization has exacerbated the negative impact of atmospheric environmental factors in urban areas, thus threatening the sustainability of future urban development. In order to ensure the sustainability of urban atmospheric environments, exploring the changing laws of urban air quality, identifying highly polluted areas in cities, and studying the relationship between air quality and land use have become issues of great concern. Based on AQI data from 340 air quality monitoring stations and urban land use data, this paper uses inverse distance weight (IDW), Getis-Ord Gi*, and a negative binomial regression model to discuss the spatiotemporal variation of air quality in the main urban area of Lanzhou and its relationship with urban land use. The results show that urban air quality has characteristics of temporal and spatial differentiation and spatially has characteristics of agglomeration of cold and hot spots. There is a close relationship between urban land use and air quality. Industrial activities, traffic pollution, and urban construction activities are the most important factors affecting urban air quality. Green spaces can reduce urban pollution. The impact of land use on air quality has a seasonal effect.
Analyzing Prospective Owners’ Choice Decision towards Plug-in Hybrid Electric Vehicles in Urban India: A Stated Preference Discrete Choice Experiment
Authors: Bera Reema , Maitra Bhargab
Published: July 2021

Plug-in Hybrid Electric Vehicles (PHEVs) can help decarbonize road transport in urban India. To accelerate the diffusion of PHEVs, investigation of commuter preferences towards the attributes of PHEVs is necessary. Therefore, the present study analyzes prospective owners’ choice decisions towards PHEVs in a typical Indian context. A stated preference survey was designed to collect responses from the current owners of conventional vehicles (CVs) in Delhi, India, and Mixed Logit (ML) models were developed to estimate commuters’ Willingness To Pay (WTP) for a set of key PHEV-specific attributes. The decomposition effect of prospective owners’ sociodemographic characteristics and trip characteristics on the mean estimates of random parameters was investigated by developing ML models with heterogeneity. Subsequently, the influence of improvement of each PHEV-specific attribute on prospective owners’ choice probability was investigated by calculating marginal effects. Among the various PHEV-specific attributes considered in the present study, high WTPs are observed for decrease in battery recharging time, reduction in tailpipe emission and increase in electric range. Therefore, an added emphasis on these attributes by vehicle manufacturers is likely to enhance the attractiveness of PHEVs to Indian commuters. The results also highlight the importance of government subsidy for promoting PHEVs in the Indian market. Prospective owners’ income, availability of home-based parking space, and average daily trip length are found to significantly influence the choice decision of Indian commuters towards PHEVs.
The Effect of Informal Gatherings on Sustainable Participation in Leisure Sport Activities: The Case of South Korea
Authors: Lee Kyung-Yur , Park Sang-Hyeon
Published: July 2021

Do informal gatherings with members in group leisure sports increase satisfaction and sustainable participation? The purpose of this study was to investigate the moderating role of informal gatherings with regard to self-determined motivation (SDM), leisure satisfaction (LS), and intention to participate sustainably (INPS) in the context of group leisure sports. An empirical analysis of a sample of 423 adults participating in group leisure sports was conducted. Structural equation modeling revealed that SDM exerted a statistically significant impact on LS, and, in turn, LS was conducive to INPS. The multigroup analysis indicated that informal gatherings played a significant moderating role in the relationship between non-self-determined extrinsic motivation and LS. Certain sociodemographic characteristics and sports participation behavior were ascertained as control variables. This study theoretically contributes to the expansion of self-determination theory, affirming the effect of informal gatherings as a unique variable and suggesting practical implications for sustainable leisure participation.
“We Make Our Community”: Youth Forging Environmental Identities in Urban Landscapes
Authors: Gallay Erin , Pykett Alisa , Flanagan Constance
Published: July 2021

Insofar as race, class, and gender have profound effects on people’s environmental experiences, and consequently their activism, the environmental field needs more work on the environmental experiences and insights of groups whose voices have been missing, including youth of color who live in urban areas in the U.S. In this paper, we focus on African American and Latinx students engaged in environmental projects in their urban communities and the impact of such projects on promoting pro-environmental leadership, agency, and behavior. We draw from written reflections and focus group interviews of several hundred 4th–12th graders (majority middle- and high-school students) who participated in place-based civic science projects. Thematic analyses of student responses found that students engaged in work on local environmental issues cultivated an appreciation for the natural world and an understanding of human-nature interdependence and the ties between the local environment and their communities’ health. Through taking action with others in their communities, students viewed themselves as contributors to their communities and started to form environmental identities in ways that are not traditionally measured. Findings point to the need for forms of environmental education that are contextually grounded and centered on environmental justice in urban areas.
Financial Institutions’ Risk Profile and Contribution to the Sustainable Development Goals
Authors: Gambetta Nicolás , Azcárate-Llanes Fernando , Sierra-García Laura , García-Benau María Antonia
Published: July 2021

This study analyses the impact of Spanish financial institutions’ risk profile on their contribution to the 2030 Agenda. Financial institutions play a significant role in ensuring financial inclusion and sustainable economic growth and usually incorporate environmental and social considerations into their risk management systems. The results show that financial institutions with less capital risk, with lower management efficiency and with higher market risk usually make higher contributions to the Sustainable Development Goals (SDGs), according to their sustainability reports. The novel aspect of the present study is that it identifies the risk profile of financial institutions that incorporate sustainability into their business operations and measure the impact generated in the environment and in society. The study findings have important implications for shareholders, investors and analysts, according to the view that sustainability reporting is a vehicle that financial institutions use to express their commitment to the 2030 Agenda and to higher quality corporate reporting.
Identifying the Positive Definiteness of Even-Order Weakly Symmetric Tensors via Z-Eigenvalue Inclusion Sets
Authors: Shen Feichao , Zhang Ying , Wang Gang
Published: July 2021

The positive definiteness of even-order weakly symmetric tensors plays important roles in asymptotic stability of time-invariant polynomial systems. In this paper, we establish two Brauer-type Z-eigenvalue inclusion sets with parameters by Z-identity tensors, and show that these inclusion sets are sharper than existing results. Based on the new Z-eigenvalue inclusion sets, we propose some sufficient conditions for testing the positive definiteness of even-order weakly symmetric tensors, as well as the asymptotic stability of time-invariant polynomial systems. The given numerical experiments are reported to show the efficiency of our results.
Municipal Solid Waste Management Practices for Achieving Green Architecture Concepts in Addis Ababa, Ethiopia
Authors: Gelan Eshetu
Published: July 2021

Solid waste is one of the social and environmental challenges that urban areas are facing. The study assesses the state of solid waste in Addis Ababa during 2016–2020 to provide implications for achieving green architecture concepts through better management of solid waste and its economic contribution. The study uses secondary and primary data. Quantitative and qualitative data are analyzed through descriptive statistics and context analysis, respectively. The result reveals that most solid waste is generated from households, followed by commercial centers, street sweeping, industries/factories, hotels, and hospitals, respectively. From 2016 to 2020, an average of 80.28% of solid waste is collected, whereas 19.72% of the waste is not collected. There are little or no efforts made to segregate solid waste at the source. The generated waste is disposed of in the Reppi open landfill. Together with Ethiopian electric power (EEP) and the City Government of Addis Ababa, waste has been converted to energy since 2019. The study suggests minimizing waste from its source by reducing generation, composting, reusing, recycling, waste-to-energy strategy, and well-designed buildings to achieve the concept of green architecture in Addis Ababa through better solid waste management.
Ochratoxins in Wines: A Review of Their Occurrence in the Last Decade, Toxicity, and Exposure Risk in Humans
Authors: Ortiz-Villeda Bryan , Lobos Olga , Aguilar-Zuniga Kateryn , Carrasco-Sánchez Verónica
Published: July 2021

Ochratoxins (OTs) are mycotoxins frequently found in wines, and their contamination can occur during any stage of the winemaking process. Ochratoxin A (OTA) has been the most widely reported and the only one whose concentrations are legislated in this beverage. However, ochratoxin B, ochratoxin A methyl ester, ochratoxin B methyl ester, ochratoxin A ethyl ester, ochratoxin B ethyl ester, ochratoxin α, ochratoxin β, OTα methyl ester, OTA ethyl amide, and OTA glucose ester have also been reported in wines. Thus, detecting only OTA would lead to the underestimation of ochratoxin levels, which is a risk to human health. Considering the threat represented by the presence of ochratoxins in wines and the long-term health problems that they can cause in wine drinkers, this paper aims to review reports of the last 10 years regarding the presence of different ochratoxins in wines and how the winemaking process influences the degree of contamination, mainly by OTA. Additionally, toxicity from human exposure due to the consumption of contaminated wines is addressed.
Human Peptides α-Defensin-1 and -5 Inhibit Pertussis Toxin
Authors: Kling Carolin , Pulliainen Arto T. , Barth Holger , Ernst Katharina
Published: July 2021

Bordetella pertussis causes the severe childhood disease whooping cough, by releasing several toxins, including pertussis toxin (PT) as a major virulence factor. PT is an AB5-type toxin, and consists of the enzymatic A-subunit PTS1 and five B-subunits, which facilitate binding to cells and transport of PTS1 into the cytosol. PTS1 ADP-ribosylates α-subunits of inhibitory G-proteins (Gαi) in the cytosol, which leads to disturbed cAMP signaling. Since PT is crucial for causing severe courses of disease, our aim is to identify new inhibitors against PT, to provide starting points for novel therapeutic approaches. Here, we investigated the effect of human antimicrobial peptides of the defensin family on PT. We demonstrated that PTS1 enzyme activity in vitro was inhibited by α-defensin-1 and -5, but not β-defensin-1. The amount of ADP-ribosylated Gαi was significantly reduced in PT-treated cells, in the presence of α-defensin-1 and -5. Moreover, both α-defensins decreased PT-mediated effects on cAMP signaling in the living cell-based interference in the Gαi-mediated signal transduction (iGIST) assay. Taken together, we identified the human peptides α-defensin-1 and -5 as inhibitors of PT activity, suggesting that these human peptides bear potential for developing novel therapeutic strategies against whooping cough.
Ellis–Bronnikov Wormholes in Asymptotically Safe Gravity
Authors: Alencar G. , Bezerra V. B. , Muniz C. R. , Vieira H. S.
Published: July 2021

In this paper, we investigate the simplest wormhole solution—the Ellis–Bronnikov one—in the context of the asymptotically safe gravity (ASG) at the Planck scale. We work with three models, which employ the Ricci scalar, Kretschmann scalar, and squared Ricci tensor to improve the field equations by turning the Newton constant into a running coupling constant. For all the cases, we check the radial energy conditions of the wormhole solution and compare them with those that are valid in general relativity (GR). We verified that asymptotic safety guarantees that the Ellis–Bronnikov wormhole can satisfy the radial energy conditions at the throat radius, r0, within an interval of values of the latter, which is quite different from the result found in GR. Following this, we evaluate the effective radial state parameter, ω(r), at r0, showing that the quantum gravitational effects modify Einstein’s field equations in such a way that it is necessary to have a very exotic source of matter to generate the wormhole spacetime–phantom or quintessence-like matter. This occurs within some ranges of the throat radii, even though the energy conditions are or are not violated there. Finally, we find that, although at r0 we have a quintessence-like matter, upon growing r, we inevitably came across phantom-like regions. We speculate whether such a phantom fluid must always be present in wormholes in the ASG context or even in more general quantum gravity scenarios.
Development of a User-Friendly Pipeline for Mutational Analyses of HIV Using Ultra-Accurate Maximum-Depth Sequencing
Authors: Meissner Morgan E. , Julik Emily J. , Badalamenti Jonathan P. , Arndt William G. , Mills Lauren J. , Mansky Louis M.
Published: July 2021

Human immunodeficiency virus type 2 (HIV-2) accumulates fewer mutations during replication than HIV type 1 (HIV-1). Advanced studies of HIV-2 mutagenesis, however, have historically been confounded by high background error rates in traditional next-generation sequencing techniques. In this study, we describe the adaptation of the previously described maximum-depth sequencing (MDS) technique to studies of both HIV-1 and HIV-2 for the ultra-accurate characterization of viral mutagenesis. We also present the development of a user-friendly Galaxy workflow for the bioinformatic analyses of sequencing data generated using the MDS technique, designed to improve replicability and accessibility to molecular virologists. This adapted MDS technique and analysis pipeline were validated by comparisons with previously published analyses of the frequency and spectra of mutations in HIV-1 and HIV-2 and is readily expandable to studies of viral mutation across the genomes of both viruses. Using this novel sequencing pipeline, we observed that the background error rate was reduced 100-fold over standard Illumina error rates, and 10-fold over traditional unique molecular identifier (UMI)-based sequencing. This technical advancement will allow for the exploration of novel and previously unrecognized sources of viral mutagenesis in both HIV-1 and HIV-2, which will expand our understanding of retroviral diversity and evolution.
Prevalence and Determinants of Vaccine Hesitancy and Vaccines Recommendation Discrepancies among General Practitioners in French-Speaking Parts of Belgium
Authors: Gobert Cathy , Semaille Pascal , Van der Schueren Thierry , Verger Pierre , Dauby Nicolas
Published: July 2021

General practitioners (GPs) play a critical role in patient acceptance of vaccination. Vaccine hesitancy (VH) is a growing phenomenon in the general population but also affects GPs. Few data exist on VH among GPs. The objectives of this analysis of a population of GPs in the Belgian Wallonia-Brussels Federation (WBF) were to: (1) determine the prevalence and the features of VH, (2) identify the correlates, and (3) estimate the discrepancy in vaccination’s behaviors between the GPs’ children and the recommendations made to their patients. An online survey was carried out among the population of general practitioners practicing in the WBF between 7 January and 18 March 2020. A hierarchical cluster analysis was carried out based on various dimensions of vaccine hesitancy: perception of the risks and the usefulness of vaccines as well as vaccine recommendations for their patients. A total of 251 GPs answered the survey. The average percentage of moderate to high vaccine hesitancy was 50.6%. Three factors were independently associated with increased risk of vaccine hesitancy: an age <50 years old, having no children, and having no contact with selected vaccine-preventable disease (measles, complicated influenza, chronic hepatitis B (HBV), bacterial meningitis, or cervical cancer) in the past 5 years. VH was associated with controversies on vaccines’ safety. GPs who had vaccinated their children against six diseases (MMR, meningococcus C (MenC), HBV, and HPV) tended not to recommend the same vaccines to their patients. Among GPs with all children vaccinated against HBV, only 37.5% recommended catch-up HBV immunization to their patients. In this small cohort of GP, moderate to high VH was associated with controversies on vaccines’ safety and with specific personal characteristics (age <50, no children, and no recent experience with a serious VPD). As previously reported, GPs have different vaccine prescription attitude toward their patients and children. These findings should be confirmed in larger cohorts.
Analysis of the Water–Energy Nexus of Treated Wastewater Reuse at a Municipal Scale
Authors: Santos Cristina , Taveira-Pinto Francisco , Pereira David , Matos Cristina
Published: July 2021

Treated wastewater has the potential to be a feasible alternative to supply non-potable uses and avoid water scarcity in urban areas, but it is important to understand and compare the associated energy consumption and CO2 emissions. This study presents a comparative analysis of the water–energy nexus associated with the traditional water supply and to the alternative reuse of treated wastewater, both for non-potable purposes. A case study of a Portuguese municipality was considered, regarding golf course irrigation and municipal gardens irrigation. A balance between production and demand was established, and the energy consumption and CO2 emissions were calculated considering the supply with drinking water and with treated wastewater. Three scenarios were defined to analyze the water–energy nexus for different configurations of the potential end-uses: (1) golf course supply, (2) municipal irrigation supply and (3) simultaneous supply to the golf course and to municipal irrigation. A quality analysis was also carried out by comparing the records from discharged wastewater quality parameters with the limits presented in the legislation for each proposed non-potable use. The results show that all scenarios present significant annual savings from using treated wastewater instead of drinking water from the public network, especially scenarios 1 and 3, that consider the golf course irrigation (water costs decrease by about 60,000.00 EUR/year). Regarding the water–energy nexus, this study reveals that treated wastewater spends less energy on its production and supply and produces fewer CO2 emissions. The energy savings can reach an average value of about kWh/year, with 5300 fewer kg of CO2 emitted in the best scenario.
Ecological Status as the Basis for the Holistic Environmental Flow Assessment of a Tropical Highland River in Ethiopia
Authors: Abebe Wubneh B. , Tilahun Seifu A. , Moges Michael M. , Wondie Ayalew , Dersseh Minychl G. , Assefa Workiye W. , Mhiret Demesew A. , Adem Anwar A. , Zimale Fasikaw A. , Abera Wuletawu , Steenhuis Tammo S. , McClain Michael E.
Published: July 2021

There is an increasing need globally to establish relationships among flow, ecology, and livelihoods to make informed decisions about environmental flows. This paper aimed to establish the ecological foundation for a holistic environmental flow assessment method in the Gumara River that flows into Lake Tana in Ethiopia and the Blue Nile River. First, the ecological conditions (fish, macro-invertebrate, riparian vegetation, and physicochemical) of the river system were characterized, followed by determining the hydrological condition and finally linking the ecological and hydrological components. The ecological data were collected at 30 sites along the Gumara River on March 2016 and 2020. River hydrology was estimated using the SWAT model and showed that the low flow decreased over time. Both physico-chemical and macroinvertebrate scores showed that water quality was moderate in most locations. The highest fish diversity index was in the lower reach at Wanzaye. Macroinvertebrate diversity was observed to decrease downstream. Both the fish and macroinvertebrate diversity indices were less than the expected maximum, being 3.29 and 4.5, respectively. The normalized difference vegetation index (NDVI) for 30 m and 60 m buffer distances from the river decreased during the dry season (March–May). Hence, flow conditions, water quality, and land-use change substantially influenced the abundance and diversity of fish, vegetation, and macroinvertebrate species. The pressure on the ecology is expected to increase because the construction of the proposed dam is expected to alter the flow regime. Thus, as demand for human water consumption grows, measures are needed, including quantification of environmental flow requirements and regulating river water uses to conserve the ecological status of the Gumara River and Lake Tana sub-basin.