bluuebunny/crossref_metadata_2025 huggingface dataset:
Reactive Oxygen Species Contribute to the Bactericidal Effects of the Fluoroquinolone Moxifloxacin in Streptococcus pneumoniae
Authors: Ferrándiz M. J. , Martín-Galiano A. J. , Arnanz C. , Zimmerman T. , de la Campa A. G.
Published: November 2015

We studied the transcriptomic response ofStreptococcus pneumoniaeto the fluoroquinolone moxifloxacin at a concentration that inhibits DNA gyrase. Treatment of the wild-type strain R6, at a concentration of 10× the MIC, triggered a response involving 132 genes after 30 min of treatment. Genes from several metabolic pathways involved in the production of pyruvate were upregulated. These included 3 glycolytic enzymes, which ultimately convert fructose 6-phosphate to pyruvate, and 2 enzymes that funnel phosphate sugars into the glycolytic pathway. In addition, acetyl coenzyme A (acetyl-CoA) carboxylase was downregulated, likely leading to an increase in acetyl-CoA. When coupled with an upregulation in formate acetyltransferase, an increase in acetyl-CoA would raise the production of pyruvate. Since pyruvate is converted by pyruvate oxidase (SpxB) into hydrogen peroxide (H2O2), an increase in pyruvate would augment intracellular H2O2. Here, we confirm a 21-fold increase in the production of H2O2and a 55-fold increase in the amount of hydroxyl radical in cultures treated during 4 h with moxifloxacin. This increase in hydroxyl radical through the Fenton reaction would damage DNA, lipids, and proteins. These reactive oxygen species contributed to the lethality of the drug, a conclusion supported by the observed protective effects of an SpxB deletion. These results support the model whereby fluoroquinolones cause redox alterations. The transcriptional response ofS. pneumoniaeto moxifloxacin is compared with the response to levofloxacin, an inhibitor of topoisomerase IV. Levofloxacin triggers the transcriptional activation of iron transport genes and also enhances the Fenton reaction.
All Three TonB Systems Are Required for Vibrio vulnificus CMCP6 Tissue Invasiveness by Controlling Flagellum Expression
Authors: Duong-Nu Tra-My , Jeong Kwangjoon , Hong Seol Hee , Nguyen Hong-Vu , Ngo Van-Hoan , Min Jung-Joon , Lee Shee Eun , Rhee Joon Haeng
Published: November 2015

TonB systems actively transport iron-bound substrates across the outer membranes of Gram-negative bacteria.
Vibrio vulnificus
CMCP6, which causes fatal septicemia and necrotizing wound infections, possesses three active TonB systems. It is not known why
V. vulnificus
CMCP6 has maintained three TonB systems throughout its evolution. The TonB1 and TonB2 systems are relatively well characterized, while the pathophysiological function of the TonB3 system is still elusive. A reverse transcription-PCR (RT-PCR) study showed that the
tonB1
and
tonB2
genes are preferentially induced
in vivo
, whereas
tonB3
is persistently transcribed, albeit at low expression levels, under both
in vitro
and
in vivo
conditions. The goal of the present study was to elucidate the raison d'être of these three TonB systems. In contrast to previous studies, we constructed in-frame single-, double-, and triple-deletion mutants of the entire structural genes in TonB loci, and the changes in various virulence-related phenotypes were evaluated. Surprisingly, only the
tonB123
mutant exhibited a significant delay in killing eukaryotic cells, which was complemented in
trans
with any TonB operon. Very interestingly, we discovered that flagellum biogenesis was defective in the
tonB123
mutant. The loss of flagellation contributed to severe defects in motility and adhesion of the mutant. Because of the difficulty of making contact with host cells, the mutant manifested defective RtxA1 toxin production, which resulted in impaired invasiveness, delayed cytotoxicity, and decreased lethality for mice. Taken together, these results indicate that a series of virulence defects in all three TonB systems of
V. vulnificus
CMCP6 coordinately complement each other for iron assimilation and full virulence expression by ensuring flagellar biogenesis.
Interaction of Mycoplasma gallisepticum with Chicken Tracheal Epithelial Cells Contributes to Macrophage Chemotaxis and Activation
Authors: Majumder Sanjukta , Silbart Lawrence K.
Published: November 2015

Mycoplasma gallisepticumcolonizes the chicken respiratory mucosa and mediates a severe inflammatory response hallmarked by subepithelial leukocyte infiltration. We recently reported that the interaction ofM. gallisepticumwith chicken tracheal epithelial cells (TECs) mediated the upregulation of chemokine and inflammatory cytokine genes in these cells (S. Majumder, F. Zappulla, and L. K. Silbart, PLoS One9:e112796,http://dx.doi.org/10.1371/journal.pone.0112796). The current study extends these observations and sheds light on how this initial interaction may give rise to subsequent inflammatory events. Conditioned medium from TECs exposed to the virulent Rlowstrain induced macrophage chemotaxis to a much higher degree than the nonvirulent Rhighstrain. Coculture of chicken macrophages (HD-11) with TECs exposed to live mycoplasma revealed the upregulation of several proinflammatory genes associated with macrophage activation, including interleukin-1β (IL-1β), IL-6, IL-8, CCL20, macrophage inflammatory protein 1β (MIP-1β), CXCL-13, and RANTES. The upregulation of these genes was similar to that observed upon direct contact of HD-11 cells with liveM. gallisepticum. Coculture of macrophages with Rlow-exposed TECs also resulted in prolonged expression of chemokine genes, such as those encoding CXCL-13, MIP-1β, RANTES, and IL-8. Taken together, these studies support the notion that the initial interaction ofM. gallisepticumwith host respiratory epithelial cells contributes to macrophage chemotaxis and activation by virtue of robust upregulation of inflammatory cytokine and chemokine genes, thereby setting the stage for chronic tissue inflammation.
Divergence between the Highly Virulent Zoonotic Pathogen Helicobacter heilmannii and Its Closest Relative, the Low-Virulence “Helicobacter ailurogastricus” sp. nov
Authors: Joosten Myrthe , Lindén Sara , Rossi Mirko , Tay Alfred Chin Yen , Skoog Emma , Padra Médea , Peters Fanny , Perkins Tim , Vandamme Peter , Van Nieuwerburgh Filip , D'Herde Katharina , Van den Broeck Wim , Flahou Bram , Deforce Dieter , Ducatelle Richard , Marshall Barry , Haesebrouck Freddy , Smet Annemieke
Published: November 2015

Helicobacter heilmannii
naturally colonizes the stomachs of dogs and cats and has been associated with gastric disorders in humans. Nine feline
Helicobacter
strains, classified as
H. heilmannii
based on
ureAB
and 16S rRNA gene sequences, were divided into a highly virulent and a low-virulence group. The genomes of these strains were sequenced to investigate their phylogenetic relationships, to define their gene content and diversity, and to determine if the differences in pathogenicity were associated with the presence or absence of potential virulence genes. The capacities of these helicobacters to bind to the gastric mucosa were investigated as well. Our analyses revealed that the low-virulence strains do not belong to the species
H. heilmannii
but to a novel, closely related species for which we propose the name
Helicobacter ailurogastricus
. Several homologs of
H. pylori
virulence factors, such as IceA1, HrgA, and jhp0562-like glycosyltransferase, are present in
H. heilmannii
but absent in
H. ailurogastricus
. Both species contain a VacA-like autotransporter, for which the passenger domain is remarkably larger in
H. ailurogastricus
than in
H. heilmannii
. In addition,
H. ailurogastricus
shows clear differences in binding to the gastric mucosa compared to
H. heilmannii
. These findings highlight the low-virulence character of this novel
Helicobacter
species.
Reciprocal Regulation ofl-Arabinose andd-Xylose Metabolism in Escherichia coli
Authors: Koirala Santosh , Wang Xiaoyi , Rao Christopher V.
Published: November 2015

Glucose is known to inhibit the transport and metabolism of many sugars inEscherichia coli. This mechanism leads to its preferential consumption. Far less is known about the preferential utilization of nonglucose sugars inE. coli. Two exceptions arel-arabinose andd-xylose. Previous studies have shown thatl-arabinose inhibitsd-xylose metabolism inEscherichia coli. This repression results froml-arabinose-bound AraC binding to the promoter of thed-xylose metabolic genes and inhibiting their expression. This mechanism, however, has not been explored in single cells. Both thel-arabinose andd-xylose utilization systems are known to exhibit a bimodal induction response to their cognate sugar, where mixed populations of cells either expressing the metabolic genes or not are observed at intermediate sugar concentrations. This suggests thatl-arabinose can only inhibitd-xylose metabolism inl-arabinose-induced cells. To understand how cross talk between these systems affects their response, we investigatedE. coliduring growth on mixtures ofl-arabinose andd-xylose at single-cell resolution. Our results showed that mixed, multimodal populations ofl-arabinose- andd-xylose-induced cells occurred at intermediate sugar concentrations. We also found thatd-xylose inhibited the expression of thel-arabinose metabolic genes and that this repression was due to XylR. These results demonstrate that a strict hierarchy does not exist betweenl-arabinose andd-xylose as previously thought. The results may also aid in the design ofE. colistrains capable of simultaneous sugar consumption.

IMPORTANCEGlucose,d-xylose, andl-arabinose are the most abundant sugars in plant biomass. Developing efficient fermentation processes that convert these sugars into chemicals and fuels will require strains capable of coutilizing these sugars. Glucose has long been known to repress the expression of thel-arabinose andd-xylose metabolic genes inEscherichia coli. Recent studies found thatl-arabinose also represses the expression of thed-xylose metabolic genes. In the present study, we found thatd-xylose also represses the expression of thel-arabinose metabolic genes, leading to mixed populations of cells capable of utilizingl-arabinose andd-xylose. These results further our understanding of mixed-sugar utilization and may aid in strain design.
Controlling the Connections of Cells to the Biofilm Matrix
Authors: Parsek Matthew R.
Published: November 2015

The importance of cyclic di-GMP (c-di-GMP) and its control of biofilm matrix assembly and production has been a focal point of researchers in recent history. In this issue, Cooley et al. (Cooley RB, Smith TJ, Leung W, Tierney V, Borlee BR, O’Toole GA, Sondermann H, J Bacteriol 198:66–77,
http://dx.doi.org/10.1128/JB.00369-15
) demonstrate that two c-di-GMP controlled features of
Pseudomonas aeruginosa
, the periplasmic protease LapG and the surface adhesin CdrA, are linked. CdrA is shown to be a substrate of LapG, with LapG activity controlled by intracellular c-di-GMP levels. This commentary discusses the significance of this finding.
Contributions of maternal and paternal adiposity and smoking to adult offspring adiposity and cardiovascular risk: the Midspan Family Study
Authors: Han T S , Hart C L , Haig C , Logue J , Upton M N , Watt G C M , Lean M E J
Published: November 2015

Objective

Obesity has some genetic basis but requires interaction with environmental factors for phenotypic expression. We examined contributions of gender-specific parental adiposity and smoking to adiposity and related cardiovascular risk in adult offspring.

Design

Cross-sectional general population survey.

Setting

Scotland.

Participants

1456 of the 1477 first generation families in the Midspan Family Study: 2912 parents (aged 45–64 years surveyed between 1972 and 1976) who had 1025 sons and 1283 daughters, aged 30–59 years surveyed in 1996.

Main measures

Offspring body mass index (BMI), waist circumference (WC), cardiometabolic risk (lipids, blood pressure and glucose) and cardiovascular disease as outcome measures, and parental BMI and smoking as determinants. All analyses adjusted for age, socioeconomic status and family clustering and offspring birth weight.

Results

Regression coefficients for BMI associations between father–son (0.30) and mother–daughter (0.33) were greater than father–daughter (0.23) or mother–son (0.22). Regression coefficient for the non-genetic, shared-environment or assortative-mating relationship between BMIs of fathers and mothers was 0.19. Heritability estimates for BMI were greatest among women with mothers who had BMI either <25 or ≥30 kg/m2. Compared with offspring without obese parents, offspring with two obese parents had adjusted OR of 10.25 (95% CI 6.56 to 13.93) for having WC ≥102 cm for men, ≥88 cm women, 2.46 (95% CI 1.33 to 4.57) for metabolic syndrome and 3.03 (95% CI 1.55 to 5.91) for angina and/or myocardial infarct (p<0.001). Neither parental adiposity nor smoking history determined adjusted offspring individual cardiometabolic risk factors, diabetes or stroke. Maternal, but not paternal, smoking had significant effects on WC in sons (OR=1.50; 95% CI 1.13 to 2.01) and daughters (OR=1.42; 95% CI 1.10 to 1.84) and metabolic syndrome OR=1.68; 95% CI 1.17 to 2.40) in sons.

Conclusions

There are modest genetic/epigenetic influences on the environmental factors behind adverse adiposity. Maternal smoking appears a specific hazard on obesity and metabolic syndrome. A possible epigenetic mechanism linking maternal smoking to obesity and metabolic syndrome in offspring is proposed. Individuals with family histories of obesity should be targeted from an early age to prevent obesity and complications.
Understanding the motivation and performance of community health volunteers involved in the delivery of health programmes in Kampala, Uganda: a realist evaluation
Authors: Vareilles Gaëlle , Marchal Bruno , Kane Sumit , Petrič Taja , Pictet Gabriel , Pommier Jeanine
Published: November 2015

Objectives

This paper presents the results of a realist evaluation that aimed to understand how, why and under what circumstances a Red Cross (RC) capacity-building intervention influences the motivation and the performance of RC community health volunteers involved in the delivery of an immunisation programme in Kampala, Uganda.

Method

Given the complexity of the intervention, we adopted realist evaluation as our methodological approach and the case study as our study design. Data collection included document review, participant observation and interviews. The constant comparative method was used for the analysis. Two contrasted cases were selected within the five Kampala districts. Each case covers the management of the immunisation programme implemented at a RC branch. In each case, a programme manager and 15 RC volunteers were interviewed. The selection of the volunteers was purposive.

Results

We found that a capacity-building programme including supervision supportive of autonomy, skills and knowledge enhancement, and adapted to the different subgroups of volunteers, leads to satisfaction of the three key drivers of volunteer motivation: feelings of autonomy, competence and connectedness. This contributes to higher retention, and better task performance and well-being among the volunteers. Enabling contextual conditions include the responsiveness of the Uganda Red Cross Society (URCS) to community needs, and recognition of the work of the volunteers, from the URCS and the community.

Conclusions

A management approach that caters for the different motivational states and changing needs of the volunteers will lead to better performance. The findings will inform not only the management of community health volunteers, but also the management of all kinds of health workers.
Prevalence, associated factors and heritabilities of metabolic syndrome and its individual components in African Americans: the Jackson Heart Study
Authors: Khan Rumana J , Gebreab Samson Y , Sims Mario , Riestra Pia , Xu Ruihua , Davis Sharon K
Published: November 2015

Objective

Both environmental and genetic factors play important roles in the development of metabolic syndrome (MetS). Studies about its associated factors and genetic contribution in African Americans (AA) are sparse. Our aim was to report the prevalence, associated factors and heritability estimates of MetS and its components in AA men and women.

Participants and setting

Data of this cross-sectional study come from a large community-based Jackson Heart Study (JHS). We analysed a total of 5227 participants, of whom 1636 from 281 families were part of a family study subset of JHS.

Methods

Participants were classified as having MetS according to the Adult Treatment Panel III criteria. Multiple logistic regression analysis was performed to isolate independently associated factors of MetS (n=5227). Heritability was estimated from the family study subset using variance component methods (n=1636).

Results

About 27% of men and 40% of women had MetS. For men, associated factors with having MetS were older age, lower physical activity, higher body mass index, and higher homocysteine and adiponectin levels (p<0.05 for all). For women, in addition to all these, lower education, current smoking and higher stress were also significant (p<0.05 for all). After adjusting for covariates, the heritability of MetS was 32% (p<0.001). Heritability ranged from 14 to 45% among its individual components. Relatively higher heritability was estimated for waist circumference (45%), high density lipoprotein-cholesterol (43%) and triglycerides (42%). Heritability of systolic blood pressure (BP), diastolic BP and fasting blood glucose was 16%, 15% and 14%, respectively.

Conclusions

Stress and low education were associated with having MetS in AA women, but not in men. Higher heritability estimates for lipids and waist circumference support the hypothesis of lipid metabolism playing a central role in the development of MetS and encourage additional efforts to identify the underlying susceptibility genes for this syndrome in AA.
Bath additives for the treatment of childhood eczema (BATHE): protocol for multicentre parallel group randomised trial
Authors: Santer Miriam , Rumsby Kate , Ridd Matthew J , Francis Nick A , Stuart Beth , Chorozoglou Maria , Wood Wendy , Roberts Amanda , Thomas Kim S , Williams Hywel C , Little Paul
Published: November 2015

Introduction

Bath emollients are widely prescribed for childhood eczema, yet evidence of their benefits over direct application of emollients is lacking.ObjectivesTo determine the clinical and cost-effectiveness of adding bath emollient to the standard management of eczema in children

Methods and analysis

Design: Pragmatic open 2-armed parallel group randomised controlled trial.Setting: General practitioner (GP) practices in England and Wales.Participants: Children aged over 12 months and less than 12 years with eczema, excluding inactive or very mild eczema (5 or less on Nottingham Eczema Severity Scale).Interventions: Children will be randomised to either bath emollients plus standard eczema care or standard eczema care only.Outcome measures: Primary outcome is long-term eczema severity, measured by the Patient-Oriented Eczema Measure (POEM) repeated weekly for 16 weeks. Secondary outcomes include: number of eczema exacerbations resulting in healthcare consultations over 1 year; eczema severity over 1 year; disease-specific and generic quality of life; medication use and healthcare resource use; cost-effectiveness. Aiming to detect a mean difference between groups of 2.0 (SD 7.0) in weekly POEM scores over 16 weeks (significance 0.05, power 0.9), allowing for 20% loss to follow-up, gives a total sample size of 423 children. We will use repeated measures analysis of covariance, or a mixed model, to analyse weekly POEM scores. We will control for possible confounders, including baseline eczema severity and child's age. Cost-effectiveness analysis will be carried out from a National Health Service (NHS) perspective.

Ethics and dissemination

This protocol was approved by Newcastle and North Tyneside 1 NRES committee 14/NE/0098. Follow-up will be completed in 2017. Findings will be disseminated to participants and carers, the public, dermatology and primary care journals, guideline developers and decision-makers.

Trial registration number

ISRCTN84102309.
Achilles tendinopathy—do plantaris tendon removal and Achilles tendon scraping improve tendon structure? A prospective study using ultrasound tissue characterisation
Authors: Masci Lorenzo , Spang Christoph , van Schie Hans T M , Alfredson Håkan
Published: November 2015

Objectives

The plantaris tendon has recently been described as a possible important factor in midportion Achilles tendinopathy. Ultrasound tissue characterisation (UTC) is a method to study tendon structure (matrix integrity). The effect of plantaris tendon removal on Achilles tendon structure was studied using UTC.

Design and setting

Prospective case series study at one centre.

Participants

Nine tendons in eight physically active and healthy patients (mean age 39 years) with chronic painful midportion Achilles tendinopathy were included. Preoperative two-dimensional ultrasound and UTC showed midportion Achilles tendinopathy (tendinosis) with medial tendon changes and suspected plantaris tendon involvement. Patients with previous operations to the Achilles tendon were excluded.

Interventions

Operative treatment consisted of excision of the plantaris tendon and scraping of the ventromedial surface of the Achilles tendon under a local anaesthetic.

Primary and secondary outcome measures

UTC examination and clinical scoring with the VISA-A questionnaire were performed preoperatively and 6 months postoperatively.

Results

At 6 months follow-up, UTC demonstrated a statistically significant (t=5.40, p<0.001) increase in the mean organised matrix (echo-type I+II) and a decrease in the mean disorganised matrix (echo-type III+IV). Seven out of eight patients were satisfied, and the VISA-A score had increased significantly (p<0.001) from 56.8 (range 34–73) preoperatively to 93.3 (range 87–100) postoperatively.

Conclusions

Excision of the plantaris tendon and scraping of the ventromedial Achilles tendon in chronic midportion tendinopathy seem to have the potential to improve tendon structure and reduce tendon pain. Studies on a larger group of patients and with a longer follow-up period are needed.
Athletes with higher VO2max present reduced oxLDL after a marathon race
Authors: Bachi André L L , Sierra Ana Paula R , Rios Francisco J O , Gonçalves Danieli A , Ghorayeb Nabil , Abud Ronaldo L , Victorino Angélica B , dos Santos Juliana M B , Kiss Maria Augusta D P , Pithon-Curi Tania C , Vaisberg Mauro
Published: November 2015

Background

During a session of prolonged and exhaustive exercise, such as a marathon race, large quantities of free radicals are produced and can oxidise (ox) several molecules, such as low-density lipoprotein (LDL). To prevent oxidative damage, athletes present higher antioxidant levels. However, the effect of marathon running on the natural IgM or IgG anti-oxLDL autoantibodies is not understood. Thus, we investigated the effect of a marathon race on oxidative stress and the mechanisms of control of this stress.

Methods

Blood samples of 20 marathon runners were collected 24 hours before, immediately and 72 hours after a marathon race to evaluate: plasma lipid profile; serum levels of oxLDL and anti-oxLDL autoantibodies (IgM and IgG isotype) and total antioxidant capacity (TAC). Maximum oxygen uptake (VO2max) was also determined.

Results

Immediately after the race, oxLDL and TAC levels decreased in comparison to the basal levels; however, the IgM or IgG anti-oxLDL levels remain unchanged. Whereas no differences were observed in the IgM or IgG anti-oxLDL levels 72h after the marathon, the oxLDL and TAC levels returned to the basal values. Significant positive correlations were observed between oxLDL and LDL-cholesterol before, and 72h after the marathon. Significant negative correlations were observed between oxLDL and VO2max immediately after the marathon and 72 h later, as well as between oxLDL and TAC 72 h after the race.

Conclusions

Athletes with a higher VO2max and total antioxidant activity presented reduced LDL oxidation. The levels of IgM or IgG anti-oxLDL autoantibodies were not affected by running the marathon.
Motocross biking for competition and for recreation: a prospective analysis of 423 injured riders
Authors: Singh Rohit , Theobald Peter , Hamad AK , Hay Stuart
Published: November 2015

Background

Motocross is a form of motorcycle racing held on established off-road circuits and has been a recreational and competitive sport across the world for over 100 years. In the UK alone, motocross has grown into a phenomenally ambitious and popular franchise. There are over 200 motocross clubs across the country, permitting over 900 events annually.

Method

Data were collected prospectively over 4 years (from 2010 to 2014) at our unit. All injuries caused by motocross biking that were referred to our trauma and orthopaedic department were included in this study, regardless of whether the rider was performing the sport competitively or recreationally.

Results

During the period studied (4 years), 423 patients were included with a total of 485 injuries, ranging from 1 to 6 injuries per patient. The patient's age range was from 4 to 73 years, with most of the injuries being sustained within the early spring and summer months representing the start of the motocross season.

Conclusions

We present the first epidemiological study of motocross injuries in the UK. Within the growing culture of ‘adrenaline sports’, motocross has become an exhilarating and extremely fashionable pastime. This study has identified and categorised the spectrum of injuries from upper limb fracture dislocations to life-threatening head and chest injuries, some of which are serious and may cause significant morbidity and possible mortality. These injuries could have significant resource implications, especially for smaller rural hospitals.
Effect of Ramadan intermittent fasting on selective fitness profile parameters in young untrained Muslim men
Authors: Roy Anindita Singha , Bandyopadhyay Amit
Published: November 2015

Background

The present study was aimed at investigating the effects of sleep deprivation and dietary irregularities during Ramadan intermittent fasting (RIF) on selective fitness profile parameters in young untrained male Muslim individuals.

Methods

77 untrained Muslim men were recruited in the study. They were divided into the experimental group (EG; n=37, age: 22.62±1.77 years) and the control group (CG; n=40, age: 23.00±1.48 years). EG was undergoing RIF while CG abstained. Aerobic fitness, anaerobic capacity or high-intensity efforts (HIEs), agility, flexibility, vertical jump height and handgrip strength were measured on 8 separate occasions—15 days before RIF, 7 days before RIF, 1st day of RIF, 7th day of RIF, 15th day of RIF, 21st day of RIF, last day of RIF and 15 days after RIF.

Results

Aerobic fitness and HIE showed a significant difference (p<0.05) during RIF in EG. Agility and flexibility score showed a significant decrease in EG during RIF, whereas changes in the vertical jump score (VJT) and handgrip strength were statistically insignificant. Studied parameters showed an insignificant variation in CG during RIF. Aerobic fitness, HIEs, agility and flexibility showed a significant intergroup variation during different experimental trials.

Conclusions

The present investigation revealed that RIF had adverse effects on aerobic fitness, HIEs, agility and flexibility of young untrained Muslims of Kolkata, India. VJT, waist-hip ratio and handgrip strength were not affected by RIF in the studied population. Mild but statistically insignificant reduction in body mass was also reflected after the mid-Ramadan week.
The challenge of preserving cardiorespiratory fitness in physically inactive patients with colon or breast cancer during adjuvant chemotherapy: a randomised feasibility study
Authors: Møller Tom , Lillelund Christian , Andersen Christina , Bloomquist Kira , Christensen Karl Bang , Ejlertsen Bent , Nørgaard Lone , Wiedenbein Liza , Oturai Peter , Breitenstein Ulla , Adamsen Lis
Published: November 2015

Introduction

Anti-neoplastic treatment is synonymous with an inactive daily life for a substantial number of patients. It remains unclear what is the optimal setting, dosage and combination of exercise and health promoting components that best facilitate patient adherence and symptom management in order to support cardio-respiratory fitness and lifestyle changes in an at-risk population of pre-illness physically inactive cancer patients.

Methods

Patients with breast or colon cancer referred to adjuvant chemotherapy and by the oncologists pre-screening verified as physically inactive were eligible to enter a randomised three-armed feasibility study comparing a 12-week supervised hospital-based moderate to high intensity exercise intervention or alternate an instructive home-based12-week pedometer intervention, with usual care.

Results

Using a recommendation based physical activity screening instrument in order to correspond with cardio-respiratory fitness (VO2 peak) proved to be an applicable method to identify pre-illness physically inactive breast and colon cancer patients. The study demonstrated convincing recruitment (67%), safety and intervention adherence among breast cancer patients; while the attendance rate for colon cancer patients was notably lower (33%). VO2-peak declined on average 12% across study groups from baseline to 12 weeks though indices towards sustaining watt performance and reduce fat mass favoured the hospital-based intervention. Pedometer use was well adapted in both breast and colon cancer patients.

Conclusions

Despite a fair adherence and safety, the current study calls into question whether aerobic exercise, regardless of intensity, is able to increase VO2-peak during texane-based chemotherapy in combination with Neulasta in physically inactive breast cancer patients.

Trial Registration:

ISRCTN24901641
Decrease in eccentric quadriceps and hamstring strength in recreational alpine skiers after prolonged skiing
Authors: Koller Arnold , Fuchs Birgit , Leichtfried Veronika , Schobersberger Wolfgang
Published: November 2015

Background

To effectively prevent injury in recreational alpine skiing, it is important to identify modifiable risk factors that can be targeted through exercise and training. Fatigue is a potential risk factor in recreational skiing, but no investigations have evaluated concentric/eccentric quadriceps and hamstring fatigue in recreational skiers. We tested the hypothesis that recreational skiing is associated with more pronounced eccentric as compared with concentric muscle fatigue.

Methods

Twenty-four healthy and fit recreational skiers (14 male and 10 female) performed an isokinetic muscle test 1 day before, 1 h after, and 24 h after a 4 h skiing session. The testing protocol consisted of concentric and eccentric quadriceps and hamstring contractions for both legs.

Results

Eccentric peak hamstring torque (both thighs) and eccentric peak quadriceps torque (left thigh) were reduced in male and female participants (p<0.05). Reduced peak torques were still present 24 h after the skiing session. There were no other significant findings.

Recreational skiing is associated with prolonged (at least 24 h) eccentric quadriceps (left thigh) and hamstring (both thighs) fatigue in men and women. Eccentric quadriceps and hamstring fatigue may be a potential injury risk factor in male and female recreational skiers. This provides some justification for judicious use of additional eccentric training modalities for alpine skiing.
Effectiveness of a single platelet-rich plasma injection to promote recovery in rugby players with ankle syndesmosis injury
Authors: Samra David J , Sman Amy D , Rae Katherine , Linklater James , Refshauge Kathryn M , Hiller Claire E
Published: November 2015

Aims

To determine whether a single ultrasound-guided platelet-rich plasma (PRP) injection into the anterior inferior tibiofibular ligament (AITFL) reduces the time for rugby athletes to return to function and match play following MRI confirmed ankle syndesmosis injury.

Methods

Cohort controlled pilot study. 10 Rugby Union players were recruited during the 2014 season, and consented to receive a single autologous PRP injection into the AITFL within 14 days of MRI confirmed ankle syndesmosis injury. A historical control group included 11 comparable Rugby Union players between 2011 and 2013 who were treated conservatively with the same inclusion criteria and rehabilitation protocol as the intervention group. Participants followed a standardised rehabilitation protocol involving simple milestones for progression. Early functional tests were performed 2 weeks after the removal of the CAM (controlled ankle motion) boot. Time to return to play was recorded. Repeat functional testing occurred within 1 week of return to play.

Results

Groups were comparable in anthropometrics, playing position and MRI injury severity. Time to return to play was significantly less in the intervention group (p=0.048). Following return to play, athletes in the intervention group showed higher agility (p=0.002) and vertical jump (p=0.001). There was a lower level of fear avoidance associated with rugby in the intervention group (p=0.014).

Conclusions

This pilot study shows that, following ankle syndesmosis injury, a single autologous PRP injection may accelerate safe and successful return to Rugby Union, with improved functional capacity and reduced fear avoidance. It demonstrates the feasibility of a randomised controlled trial to further assess this therapy.

Trial registration number

ANZCTRN12614000055606.
Mechanical efficiency improvement in relation to metabolic changes in sedentary obese adults
Authors: Jabbour Georges , Iancu Horia-Daniel
Published: November 2015

Purpose

Mechanical efficiency (ME) refers to the ability of an individual to transfer energy consumed by external work. This performance indicator is impaired by obesity and is associated with decreased high-intensity exercise performance. However, it is unclear if ME may be improved in response to high intensity training (HIT). This study aimed to determine if ME increases in response to HIT in obese adults and to identify the factors associated with these changes.

Methods

24 obese adults (body mass index=∼33 kg/m2) were randomised into control (n=12) and trained (n=12) groups. Following baseline metabolic, anthropometric, fitness and ME measurements, the participants completed a 6-week exercise intervention that included 18 sessions of six repeats of 6 s supramaximal sprints on an electromagnetically braked cycle ergometer. The metabolic, anthropometric and fitness assessments were repeated postintervention. ME (expressed as a %) was calculated during an incremental maximal cycling test at stages of 25, 50, 75, 100 and 125 W.

Results

ME did not differ across the groups at 25 and 50 W. Following HIT, ME increased significantly at 75, 100 and 125 W (p<0.01, respectively) compared with the control group (p<0.01, respectively). Although no changes in fat-free mass were observed following HIT, the increases in ME at 75, 100 and 125 W correlated positively with both homeostasis model assessment-estimated insulin resistance index decreases (r=0.9; r=0.89 and r=0.88, p<0.01, respectively) and peak power increases (r=0.87, r=0.88 and r=0.9, p<0.01, respectively).

Conclusions

Although there were no changes in the participants’ anthropometric variables, HIT improved ME in obese adults, an enhancement that appears to be related to increases in muscle strength and metabolic adaptations.
Susceptibility to exertional heat illness and hospitalisation risk in UK military personnel
Authors: Stacey Michael J , Parsons Iain T , Woods David R , Taylor Peter N , Ross David , J Brett Stephen
Published: November 2015

Background

Susceptibility to exertional heat illness (EHI) is considered multifactorial in nature. The aims of this study were to (1) review traditional susceptibility factors identified in cases of EHI and (2) determine how they are related to risk of hospitalisation.

Methods

Review of an electronic database of EHI reported in the British Army between 1 September 2007 and 31 December 2014. Cases were categorised by demographic, situational and susceptibility variables. Univariate and multivariate logistic regression was performed for the OR for hospitalisation by risk factor.

Results

361 reports were included in the analysis. 33.5% of cases occurred in hot climates, 34.6% in temperate climates during summer months and 31.9% in temperate climates outside of summer months. Traditional susceptibility factors were reported in 193 but entirely absent from 168 cases. 137 cases (38.0%) were admitted to hospital. Adjusted OR for hospitalisation was lower for recruits (OR 0.42, 95% CI 0.18 to 0.99, p<0.05) and for personnel wearing occlusive dress (OR 0.56, 95% CI 0.34 to 0.93, p<0.05) or unacclimatised to heat (OR 0.31, 95% CI 0.15 to 0.66, p<0.01).

Conclusions

The global, year-round threat of EHI is highlighted. Absence of susceptibility factors in nearly half of reports highlights the challenge of identifying EHI-prone individuals. Paradoxical association of traditional susceptibility factors with reduced hospitalisation risk may reflect the contemporary contexts in which severe EHI occurs. These findings also suggest a need for better evidence to inform guidelines that aim to prevent severe EHI concurrent to reducing overall morbidity.
Maternal inflammation during late pregnancy is lower in physically active compared with inactive obese women
Authors: Tinius Rachel A. , Cahill Alison G. , Strand Eric A. , Cade W. Todd
Published: November 2015

The primary purpose of this study was to compare maternal plasma inflammation between physically active and inactive obese women during late pregnancy. The secondary purpose was to examine the relationships between maternal plasma inflammation and lipid metabolism and maternal and neonatal metabolic health in these women. A cross-sectional, observational study design was performed in 16 obese-inactive (OBI; means ± SD; age, 25.0 ± 4.8 years; prepregnancy body mass index (BMI), 36.3 ± 4.3 kg/m2; body fat percentage in late gestation, 37.7% ± 3.5%) and 16 obese-active (OBA; age, 28.9 ± 4.8 years; prepregnancy BMI, 34.0 ± 3.7 kg/m2; body fat in late gestation, 36.6% ± 3.8%) women during the third trimester of pregnancy. Maternal plasma inflammation (C -reactive protein (CRP)) and insulin resistance (Homeostatic Model Assessment-Insulin Resistance) were measured at rest. Plasma lipid concentration and metabolism (lipid oxidation and lipolysis) were measured at rest, during a 30-min bout of low-intensity (40% peak oxygen uptake) exercise, and during a resting recovery period using indirect calorimetry. Umbilical cord blood was collected for measurement of neonatal plasma insulin resistance, inflammation, and lipid concentration. Neonatal body composition was measured via air displacement plethysmography. Maternal plasma CRP concentration was significantly higher in OBI compared with OBA women (9.1 ± 4.0 mg/L vs. 6.3 ± 2.5 mg/L, p = 0.02). Maternal plasma CRP concentration was significantly associated with maternal lipolysis (r = 0.43, p = 0.02), baseline lipid oxidation rate (r = 0.39, p = 0.03), and baseline plasma free fatty acid concentration (r = 0.36, p = 0.04). In conclusion, maternal physical activity may reduce inflammation during pregnancy in obese women. Maternal lipid metabolism is related to systemic inflammation.
Biochemical and biological activity of arginine deiminase fromStreptococcus pyogenesM22
Authors: Starikova Eleonora A. , Sokolov Alexey V. , Vlasenko Anna Yu. , Burova Larisa A. , Freidlin Irina S. , Vasilyev Vadim B.
Published: November 2015

Streptococcus pyogenes (group A Streptococcus; GAS) is an important gram-positive extracellular bacterial pathogen responsible for a number of suppurative infections. This micro-organism has developed complex virulence mechanisms to avoid the host’s defenses. We have previously reported that SDSC from GAS type M22 causes endothelial-cell dysfunction, and inhibits cell adhesion, migration, metabolism, and proliferation in a dose-dependent manner, without affecting cell viability. This work aimed to isolate and characterize a component from GAS type M22 supernatant that suppresses the proliferation of endothelial cells (EA.hy926). In the process of isolating a protein possessing antiproliferative activity we identified arginine deiminase (AD). Further study showed that this enzyme is most active at pH 6.8. Calculating Kmand Vmaxgave the values of 0.67 mmol·L–1and 42 s−1, respectively. A distinctive feature of AD purified from GAS type M22 is that its optimum activity and the maximal rate of the catalytic process is close to neutral pH by comparison with enzymes from other micro-organisms. AD from GAS type M22 suppressed the proliferative activity of endothelial cells in a dose-dependent mode. At the same time, in the presence of AD, the proportion of cells in G0/G1phase increased. When l-Arg was added at increasing concentrations to the culture medium containing AD (3 μg·mL–1), the enzyme’s capacity to inhibit cell proliferation became partially depressed. The proportion of cells in phases S/G2increased concomitantly, although the cells did not fully recover their proliferation activity. This suggests that AD from GAS type M22 has potential for the suppression of excessive cell proliferation.
Precise determination of aquatic plant wet mass using a salad spinner
Authors: Bickel Tobias Oliver , Perrett Christine
Published: November 2015

The reliable assessment of macrophyte biomass is fundamental for ecological research and management of freshwater ecosystems. While dry mass is routinely used to determine aquatic plant biomass, wet (fresh) mass can be more practical. We tested the accuracy and precision of wet mass measurements by using a salad spinner to remove surface water from four macrophyte species differing in growth form and architectural complexity. The salad spinner aided in making precise and accurate wet mass with less than 3% error. There was also little difference between operators, with a user bias estimated to be below 5%. To achieve this level of precision, only 10–20 turns of the salad spinner are needed. Therefore, wet mass of a sample can be determined in less than 1 min. We demonstrated that a salad spinner is a rapid and economical technique to enable precise and accurate macrophyte wet mass measurements and is particularly suitable for experimental work. The method will also be useful for fieldwork in situations when sample sizes are not overly large.
Distant effects of unilateral renal ischemia/reperfusion on contralateral kidney but not lung in rats: the roles of ROS and iNOS
Authors: Fatemikia Hossein , Ketabchi Farzaneh , Karimi Zynab , Moosavi Seyed Mostafa Shid
Published: November 2015

Acute kidney injury is usually associated with distant organ dysfunction. The roles of inducible nitric oxide synthase (iNOS) and reactive oxygen species (ROS) in this phenomenon were investigated following 2 h unilateral renal ischemia and 24 h reperfusion. There were 3 groups of rats subjected to either unilateral ischemia/reperfusion (UIR group), unilateral nephrectomy (UNX group), or sham operation. Two further groups were given α-tocopherol and aminoguanidine with UIR (treated-UIR group) and UNX (treated-UNX group). Plasma nitrite/nitrate and malondialdehyde were elevated only in the UIR group. Creatinine clearance and blood flow increased in non-ischemic kidney of the UIR, but not to the same extent as remnant kidney of the UNX group, while they had equal compensatory rises in absolute Na+ and K+ excretion and urine flow. Non-ischemic kidney of the treated-UIR group, but not remnant kidney of the treated-UNX group, showed more elevation in blood flow, whereas both kidneys had reductions in absolute Na+ excretion and urine flow. Respiratory functional variable were not different between all groups. Therefore, 2 h unilateral renal ischemia and 24 h reperfusion did not affect lung but had distant effects on contralateral kidney partly mediated by ROS and NO-derived from iNOS to dampen compensatory increases in renal hemodynamics and to decrease tubular reabsorption.
Intraplate seismicity in mid-plate South America: correlations with geophysical lithospheric parameters
Authors: Agurto-Detzel Hans , Assumpção Marcelo , Bianchi Marcelo , Pirchiner Marlon
Published: November 2015

Mid-plate South America remains one of the least-studied regions of intraplate seismicity. Little is known about the origin and controlling factors that make this area the least seismically active intraplate region in the world. We analysed the distribution of intraplate seismicity and its correlation with several geophysical lithospheric parameters in an attempt to establish which factors might promote or inhibit the occurrence of intraplate earthquakes. We found that above-average seismicity occurs mostly in Neoproterozoic fold belts, associated with areas having a positive gravity anomaly, lower elastic thickness, higher heat flow, thinned crust and a negative S-wave anomaly at 100 km depth (associated with non-cratonic crust). Cratonic areas with a higher elastic thickness and lower heat flow are associated with low rates of seismicity. Our study suggests that the most important controlling factors are elastic thickness and heat flow. We propose that earthquake-prone areas with these favourable conditions correspond to regions of weakened lithosphere, where most of the regional lithospheric stresses are supported by the overlying brittle upper crust. These areas act as local concentrators of the regional compressional stress field, with the stress build-up then leading to the occurrence of intraplate seismicity.

Supplementary material:
contains additional statistics and figures considering different filters for the used catalogue as a mean of comparison with the figures presented in the main text. They are available at
http://www.geolsoc.org.uk/SUP18872
The Neural Basis of Long-Distance Navigation in Birds
Authors: Mouritsen Henrik , Heyers Dominik , Güntürkün Onur
Published: November 2015

Migratory birds can navigate over tens of thousands of kilometers with an accuracy unobtainable for human navigators. To do so, they use their brains. In this review, we address how birds sense navigation- and orientation-relevant cues and where in their brains each individual cue is processed. When little is currently known, we make educated predictions as to which brain regions could be involved. We ask where and how multisensory navigational information is integrated and suggest that the hippocampus could interact with structures that represent maps and compass information to compute and constantly control navigational goals and directions. We also suggest that the caudolateral nidopallium could be involved in weighing conflicting pieces of information against each other, making decisions, and helping the animal respond to unexpected situations. Considering the gaps in current knowledge, some of our suggestions may be wrong. However, our main aim is to stimulate further research in this fascinating field.