bluuebunny/crossref_metadata_2025 huggingface dataset:
Tik Tok Media Pengembangan Karakter Melalui Kreativitas Tari Dayak
Authors: Purnama Asri Adinda Gita Kinanthi , Suwarjiya  , Aulia Nur
Published: September 2022

Fungsi aplikasi TikTok tidak dapat dipandang sebelah mata dan mampu berperan dalam mencapai tujuan pendidikan. Tujuan penelitian ini untuk mendeskripsikan nilai karakter pendidikan yang muncul melalui hasil kreativitas tari kreasi Dayak dengan pemanfaatan aplikasi Tik-tok sebagai media pengembangan karakter siswa ekstrakurikuler di SMPN 1 Kapuas Murung. Jenis penelitian eksperimen dengan pendekatan kuantitatif dan kualitatif. Pengumpulan data dengan teknik wawancara, observasi, dokumentasi, tes dan studi pustaka. Penelitian menghasilkan 2 tari kreasi Dayak yang bertema pada pengembangan Permainan Tradisional berjudul tari Ular Naga dan tari Inting yang hasilnya diupload di aplikasi Tik Tok.(2) Terdapat 12 nilai karakter yang muncul yaitu karakter cinta tanah air, kreatif, disiplin, mandiri, jujur, kerja keras, demokratif, rasa ingin tahu, bersahabat/komunikatif, menghargai prestasi, peduli sosial, dan tanggung jawab.(3)Aplikasi Tik Tok sebagai aplikasi yang digemari kalangan peserta didik, mampu mendukung berbagai karakter positif melalui hasil karya kreatif dan efektif sebagai ruang ekspresi dan apresiasi.

 

Kata Kunci: tik tok, pengembangan karakter, kreativitas, tari kreasi, dayak
Acceptance Analysis of School DAPODIK Information System Using the Technology Acceptance Model (TAM)
Authors: Akhmad Syarwani  , Kenti Yuliana
Published: September 2022

The purpose of this study was to determine user acceptance of the Basic Education Data system (DAPODIK) with the Technology Acceptance Model (TAM)  based on five constructs. The five constructs are Perceived Usefulness, Perceived  Ease Of Use, Attitude Towards Behavior, Behavioral Intention, and Actual Technology Use. This study will analyze the relationship between constructs that affect the acceptance and use of DAPODIK in Elementary School District Binuang.

The type of research used in this research is explanatory research with data analysis techniques using the SEM-PLS approach, analyzed using SmartPLS 3.0 software . There are 62 samples of respondents from teachers at the District Binuang  Elementary School in Indonesia.

The conclusion of this study is that of the seven hypotheses proposed, there are six accepted hypotheses and one rejected hypothesis, namely: Perceived Ease Of Use affects Perceived Usefulness, Perceived Ease Of Use has an effect on Attitude Towards Behavior, Perceived Usefulness has an effect on Attitude Towards Behavior, Perceived Usefulness has no effect on Behavioral Intention, Perceived Usefulness has an effect on Actual Technology Use, Attitude Towards Behavior has an effect on Behavioral Intention, and Behavioral Intention has an effect on Actual Technology Use.
Students’ Motivation in Offline Learning Post Covid-19
Authors: Syakir Akhmad Syakir , Rizki Nurwahyuni
Published: September 2022

Abstract: The return of the education system to face-to-face learning had an impact on students' learning habits. This study aimed to perceive the students’ intrinsic motivation in offline learning post-COVID-19. It focused on instrumental motivation. The subjects of this study included 25 new students from Canada's group of Intensive English Classes at the University of Muhammadiyah Banjarmasin toward their offline learning post-Covid-19. Observations and questionnaires were used to analyze the data in a descriptive qualitative manner. The mean score of the students' intrinsic motivation was discovered to 71,3. It means that the students in the Intensive English Class have an intermediate intrinsic motivation for learning English.

 

Keywords: Students’ motivation, intrinsic, offline learning.
Tailoring Education for the Future of Work Post Covid-19
Authors: Committee ISETA
Published: September 2022

1st International Seminar on Education, Technology and Arts.

Theme: Tailoring Education for the Future of Work Post Covid-19
Kearifan Lokal dalam Kumpulan Puisi Banjar sebagai Media Pembelajaran pada Sekolah Menengah
Authors: Komalasari Ida , Rusdiana Ida , Halisa Eva Nor
Published: September 2022

Local wisdom is a system of values ​​and norms that are compiled, adhered to, understood, and applied by local communities based on their understanding and experience in interacting with the environment. Local wisdom is very important to be learnt because it functions as 1) conservation of natural resources, 2) human resource development 3) cultural development 4) development and science 5) ethics and morals, and so on. This research is focused on 1) the form of local wisdom found in Banjar poetry collection and 2) how to apply local wisdom in Banjar poetry collection as a medium of learning in secondary schools. The method of the research is descriptive analysis. The data sources of this research are 1) a collection of Banjar poetry and 2) teachers and students of Madrasah Aliyah Muhamadiyah 1 Banjarmasin. Data collection techniques used in this research are text observations, field observations, interviews and questionnaires. The results of the research are 1) the local wisdom found in Banjar poetry collection are in the form of beliefs, customary laws, and norms. It was found that local community’s belief of their ancestors in Meratus forest is still strong. The people believe that Meratus has its own “guard” called Panglima Burung, he is accompanied by traditional music called kuriding. The local community also believe in Panglima Angsa for their guidance in life, the people often ask for his wisdom. The local community's customary law found in Banjar poetry collection is the presence of ancestral sabers called mandau that are flown in when the community wants to cut tyranny for the loss of their homes in Meratus forest. The norm is in the form of an appeal to preserve nature which is called Bawanang. Bawanang is seen as an ancestor who is able to overcome all environmental damage by using supernatural powers. The community also believe that Bawanang is capable to drive away greedy people who damage the environment and 2) Learning local wisdom through Banjar poetry collection can be done by a) asking students to analyse the atmosphere, theme, and meanings of local wisdom found in Banjar poetry collection and b) asking students to demonstrate local wisdom found in Banjar poetry collection by applying the norms that exist in the poetry to their daily life activities as the norms are considered to be some messages from the ancestors to maintain preservation of nature.
The Analogy of Magnet Rules to Practice Main Verbs and Helping Verbs in English Sentence Predicate
Authors: Suciati Nana , Palupi Tri Winindyasari
Published: September 2022

The rule of magnet is the basic condition of every magnet. It has north and south poles.  When the north pole of one magnet is closed to the south pole of another magnet, they will pull each other. On the opposite, when north pole is closed to north pole, or south pole is closed to south pole, they will repel each other. These basic rules of magnet can be applied to help students to understand developing English sentence predicate, especially when the sentences are the result of translation from Bahasa Indonesia. The translation result is sometimes not accepted grammatically. It seems that our students still making mistakes when it comes to use helping verbs and main verbs in their sentences.  This study is to propose the option of using magnet rules as a teaching trick to help students making sentences in English grammatically by using analogy of magnet rules for main verbs and helping verbs in English.
Analisis Kesulitan Pemahaman Menulis Cerita Dongeng Pada Pembelajaran E-Learning Bahasa Indonesia Menggunakan Media Zoom Meeting Di Kelas II SD IT Muhammadiyah Palangkaraya
Authors: Rini Rini
Published: September 2022

The purpose of this research was to determine the difficulty of understanding student fairy-tale stories during long distance learning using zoom meetings. The method used to do this research is a qualitative method with descriptive type. As for data collection techniques is observation, and interviews. Analysis of research data uses stages consisting of data reduction, display, data collection and verification. Based on the research discovery, it can be concluded, namely (1) there are some students who do not understand the material to write fairy tales. so students are still not able to write fairy tales well (2) found several factors The causes include the lack of student learning interest due to the learning process online, the teacher in delivering the material is still not optimal due to the constraints of signals, school facilities and infrastructure that are inadequate, especially for long-distance learning activities (e-learning).
Peran Kepala Sekolah dalam Meningkatkan Profesionalisme Guru
Authors: Huriaty Dina , Esterani Zefani , Saufi Muhammad
Published: September 2022

This study aimed to describe the role of the headmaster and describe the supporting and inhibiting factors in increasing teachers’ professionalism at Elementary School. This study used descriptive qualitative approach. The subjects of this research were the school headmaster and two teachers of Elementary School in Banjarmasin. Data collection were documentation, observation, and interviews. Data analyses were carried out by collecting data, reducing data, presenting data, and drawing conclusions. Validation of data was tested by using source triangulation. The results of this research show that the role of the headmaster in increasing teachers’ professionalism is as follows. (1) The headmaster carries out his role following his responsibilities as an educator, manager, supervisor, leader, innovator, and motivator to increase teachers’ professionalism but has not run optimally. (2) Supporting factors are competence teachers, a representative school environment, learning infrastructure availability, and teachers participating in activities to support their abilities as educators. (3) The inhibiting factors are the lack of teachers' understanding of technology and the lack of socialization on regulation and policies in the school environment
Analysis Of Students' Critical Thinking Skills On The Material Of Flat Shapes In The Fifth Grade Of Tindoi State Elementary School
Authors: La Ili  , Yoo Eka Yana Kansil  , Wa Ode Nur Esa  , Cici Sarfian
Published: September 2022

The ability to think critically is one of the abilities that must be mastered by students and has an important role in the mathematics learning process. Based on initial observations, it shows that the critical thinking skills of fifth graders at SD Negeri Tindoi are still low. This study aims to analyze students' critical thinking skills on the flat-shaped material. This research is a qualitative research. This study uses 4 indicators of critical thinking skills according to Robert Ennis, namely providing simple explanations, making further explanations, determining strategies and tactics, and concluding. The subjects in this study were 9 students of class V SD Negeri Tindoi, consisting of 3 male students and 6 female students. Data collection was carried out by written tests, interviews, and document studies. Data analysis techniques used are data reduction, data presentation, and drawing conclusions. The technique of checking the validity of the data is using the triangulation technique. Based on the results of the study, it was shown that the critical thinking skills of the fifth grade students of SD Negeri Tindoi belonged to the low category. This can be seen by the number of students who have low critical thinking skills as many as 4 students, 2 students with sufficient critical thinking skills, and 3 students with very low critical thinking skills out of 9 students. The students' critical thinking ability on each indicator in solving the problem of flat shape material shows different results in each category of critical thinking ability, namely: 1) Students with sufficient critical thinking ability have been able to meet three indicators of critical thinking ability, namely the indicator provides an explanation. simple, determine strategies and tactics and conclude. 2) Students with sufficient critical thinking skills have been able to meet two indicators of critical thinking skills, namely indicators of providing simple explanations, determining strategies and tactics, and 3) Students with very low critical thinking skills are only able to meet one indicator of critical thinking skills, namely indicators provide a simple explanation.
Time-dependent optimized coupled-cluster method with doubles and perturbative triples for first principles simulation of multielectron dynamics
Authors: Pathak Himadri , Sato Takeshi , Ishikawa Kenichi L.
Published: September 2022

We report the formulation of a new, cost-effective approximation method in the time-dependent optimized coupled-cluster (TD-OCC) framework [T. Sato et al., J. Chem. Phys. 148, 051101 (2018)] for first-principles simulations of multielectron dynamics in an intense laser field. The method, designated as TD-OCCD(T), is a time-dependent, orbital-optimized extension of the “gold-standard” CCSD(T) method in the ground-state electronic structure theory. The equations of motion for the orbital functions and the coupled-cluster amplitudes are derived based on the real-valued time-dependent variational principle using the fourth-order Lagrangian. The TD-OCCD(T) is size extensive and gauge invariant, and scales as O(N7) with respect to the number of active orbitals N. The pilot application of the TD-OCCD(T) method to the strong-field ionization and high-order harmonic generation from a Kr atom is reported in comparison with the results of the previously developed methods, such as the time-dependent complete-active-space self-consistent field (TD-CASSCF), TD-OCC with double and triple excitations (TD-OCCDT), TD-OCC with double excitations (TD-OCCD), and the time-dependent Hartree-Fock (TDHF) methods.
Surgical treatment strategies for patients with type A aortic dissection involving arch anomalies
Authors: Zhu Jiade , Tong Guang , Zhuang Donglin , Yang Yongchao , Liang Zhichao , Liu Yaorong , Yu Changjiang , Zhang Zhen , Chen ZeRui , Liu Jie , Yang Jue , Li Xin , Fan Ruixin , Sun Tucheng , Wu Jinlin
Published: September 2022

Objective

The aim of the study was to investigate surgical modalities and outcomes in patients with type A aortic dissection involving arch anomalies.

Method

Patients with type A aortic dissection who underwent surgical treatment at our center between January 2017 and 31 December 2020 were selected for this retrospective analysis. Data including computed tomography (CT), surgical records, and cardiopulmonary bypass records were analyzed. Perioperatively survived patients were followed up, and long-term mortality and aortic re-interventions were recorded.

Result

A total of 81 patients with arch anomalies were included, 35 with “bovine” anomalies, 23 with an aberrant right subclavian artery, 22 with an isolated left vertebral artery, and one with a right-sided arch + aberrant left subclavian artery. The strategies of arch management and cannulation differed according to the anatomic variation of the aortic arch. In total, seven patients (9%) died after surgery. Patients with “bovine” anomalies had a higher perioperative mortality rate (14%) and incidence of neurological complications (16%). Overall, four patients died during the follow-up period, with a 6-year survival rate of 94.6% (70/74). A total of four patients underwent aortic re-intervention during the follow-up period; before the re-intervention, three received the en bloc technique (13.6% 3/22) and one received hybrid therapy (11.1% 1/9).

Conclusion

With complete preservation and reconstruction of the supra-arch vessels, patients with type A aortic dissection combining arch anomalies can achieve a favorable perioperative prognostic outcome. Patients who received the en bloc technique are more likely to require aortic re-intervention than patients who underwent total arch replacement with a four-branched graft vessel. Cannulation strategies should be tailored according to the variation of anatomy, but routine cannulation with the right axillary artery can still be performed in most patients with arch anomalies, even for patients with an aberrant right subclavian artery.
A novel two-phase load compensation method under unbalanced voltages for renewable energy integration
Authors: Zhang Yuanshi , Wang Liwei , Struthers Chris , Hu Qinran
Published: September 2022

This paper explores new design methods for correcting unbalanced power distribution networks in order to improve the power quality and reliability for sensitive industrial loads. While traditional compensators typically need to be connected to all the three phases of the AC network, this paper proposes a novel three-phase voltage balancing compensator that can operate with a connection to only two of the three phases. The new concept is based on the phasor symmetrical component theory, which can be implemented using either mechanically switched reactors (MSRs), TCRs (thyristor-controlled reactors) or VSCs (Voltage source converters). Three methods are presented to calculate the value of the proposed two-phase compensator, i.e., discrete solution, analytical solution and novel simplified solution. The discrete solution is solved via the exhaustive search method, which was successfully used by StruthersTech to correct the power quality of an unbalanced industrial power system. The analytical solution is based on the nodal voltage of analysis method. The simplified solution derives a practical albeit approximated relationship between the negative sequence source voltage and zero sequence load voltage, thus avoiding the need to solve nonlinear equations. Dynamic simulations are implemented using MATLAB/Simulink Simscape Blockset to validate the effectiveness of the proposed two-phase voltage balancing method.
Improving artemisinin and essential oil production from Artemisia plant through in vivo elicitation with gamma irradiation nano-selenium and chitosan coupled with bio-organic fertilizers
Authors: Sayed Tarek E. , Ahmed El-Sayed S.
Published: September 2022

Artemisia plants process potential bioactive secondary metabolites such as artemisinin and essential oil. They are useful in controlling potential pests and microbes and have a therapeutic effect. The commercial production of artemisinin and essential oil is limited to regarding the worldwide demand. Urgent attempts must be undertaken to improve the production of bioactive secondary metabolites. The objectives of this experiment were to increase the production and improve the quality of bioactive secondary metabolites in order to limit the use of agrochemicals. Therefore, a field experiment was conducted during the 2018 and 2019 seasons. Treatments included three elicitations (gamma irradiation, nano-selenium, and chitosan) and three kinds of fertilizers (NPK, Moringa leaf extract, and humic acid). The experiment was conducted as a factorial with a completely randomized block. The designs and treatments were arranged in a split–split plot with three replicates. A single application of elicitors showed that chitosan > nano-selenium, chitosan > gamma irradiation, and Moringa > humic acid > NPK in plants’ artemisinin and essential oil content, while the interaction showed a significant synergistic relationship between elicitors and the fertilizers in enhancing the quantity and quality of artemisinin and essential oil of Artemisia plants. Without using any pesticides, there was no infection that appeared in Artemisia plants, this could be due to the enhancement of bioactive secondary metabolite production.
Ozone concentration at various heights near the surface layer in Shenyang, Northeast China
Authors: Li Liguang , Liu Ningwei , Shen Lidu , Zhao Ziqi , Wang Hongbo , Wang Yangfeng , Li Xiaolan , Ma Yanjun
Published: September 2022

Ozone pollution has been growing in the recent decade, becoming a critical urban environmental issue in China. However, Shenyang’s near-surface ozone concentration (ground to 100 m altitude) is of grave concern. To obtain the ozone concentration and the corresponding meteorological conditions from 1 October 2018, to 30 September 2019, we built observatory sites at low (≈15 m) and high (≈90 m) layers in Shenyang, northeast China. Then, we analyzed the temporal variation of ozone concentration and discussed the relationship between ozone concentration and the meteorological factors at low and high layers. Compared with the high layer, the ozone concentration in the low layer is chartered with an earlier peak and higher diurnal range. The ozone concentration in all seasons first peaked at a low-layer height, in early in winter (15:00) and late summer (17:00). Then, the peak at high-layer height lagged for behind 1–2 h, generally occurring between 17:00 and 19:00. The variations in ozone concentration at low and high layers differed in daytime and nighttime. The diurnal ranges of ozone concentrations at the low layer were higher than those at the high layer in the four seasons examined. Overall, ozone concentration correlated significantly with air temperature, having correlation coefficients of 0.814 (p < 0.05) in the low layer and 0.731 (p < 0.05) in the high layer. Inversely, it showed a weak correlation with relative humidity: 0.310 (p < 0.05) and 0.351 (p < 0.05) in the low and high layers, respectively. Also, the correlation coefficients with wind speed were meager at both layers. Generally, ozone pollution is not severe in Shenyang.
Impact of urbanization and economic growth on environmental quality in western africa: Do manufacturing activities and renewable energy matter?
Authors: Radoine Hassan , Bajja Salwa , Chenal Jerome , Ahmed Zahoor
Published: September 2022

The countries in Western Africa have the greatest potential for renewable energy generation in the world and are likely to be vulnerable to the adverse effects of climate change. Unfortunately, only a few of them have tapped into this potential, as non-renewable energy still dominates the total energy mix of these countries. The present study explores the effect of economic growth, manufacturing value-added, urbanization, financial development, foreign direct investment, and renewable energy consumption on the environmental quality in West African countries by using a sample of some selected countries over the period 1991–2018. This study is analyzed by adopting the method of Driscoll-Kraay (DK) panel regression. The panel result suggests that manufacturing value-added, urbanization, financial development, and foreign direct investment increase environmental degradation. In addition, the findings of this study reveal that economic growth and renewable energy consumption contribute, significantly, to environmental quality. Bidirectional causality exists between economic growth and CO2 emissions and between urbanization and environmental degradation. Accordingly, in order to improve environmental quality in these countries, it is imperative to embrace and promote the consumption of clean energy sources, which will contribute more to the promotion and adoption of renewables in this strategic region.
In-situ synthesis of 3D BiOBr/UiO-66-NH2 heterojunction nanocomposite and its excellent photocatalytic degradation of rhodamine B dye
Authors: Feng Shuna , Yan Zheng , Ni Qianqian , Zhang Yun
Published: September 2022

As a primary goal, The fast recombination of stimulated excitons and poor visible light response are key problems in the photocatalysis field. Combining with materials with good visible light responses to form heterogeneous structures is still the most effective way. In this study, BiOBr/UiO-66-NH2 photocatalyst was synthesized by a simple method. BiOBr is a 3D spherical structure stacked by nanosheets, which enhance its adsorption capacity for pollutants and catalyze the dark reaction to reach the equilibrium point for too long. Coupling with UiO-66-NH2, its adsorption capacity was inhibited and the dark reaction time was shortened. The excellent removal rate of Rhodamine B (RhB) by BiOBr/UiO-66-NH2 reached 96.79% after 30 min of visible light irradiation and 99.37% after 90 min. With an increase in pollutant concentration, the composite still showed excellent photocatalytic performance. This study also tried to abandon the dark adsorption stage and directly degraded RhB efficiently and quickly under visible light. Finally, the photocatalytic enhancement mechanism was proposed.
Space use and microhabitat selection of wild giant pandas in Meigu Dafengding National Nature Reserve, China
Authors: Chen Yuxiang , Wang Xiao , Zheng Xiaoguang , Gong Yihua , Chen Minghua , Qiu Lan , Zhou Hong , Wei Wei , Han Han
Published: September 2022

The giant panda (Ailuropoda melanoleuca) is a special rare and endangered species in China and is the flagship species for global biodiversity conservation. This study used different methods including Kernel density, statistical analysis, and logistic regression analysis to investigated the spatial distribution, migration in different seasons and different distribution areas, as well as the foraging patch selection strategies of wild giant pandas in Meigu Dafengding National Nature Reserve in the Liangshan Mountains. The results demonstrated that, in the study area, giant pandas were mainly distributed in the Wahei-Yizi Yakou Habitat Corridor with an activity area of 144.93 km2, which accounted for 28.59% of the whole reserve. The core distribution area of the species was 92.07 km2 and the population density was 0.24 pcs km–2. There were two significant seasonal domains for the activities of giant pandas: the low-altitude winter habitat of Yushania maculata Yi and Yushania ailuropodina Yi bamboo species, as well as the high-altitude summer habitat of Bashania fangiana species. With seasonal changes, giant pandas migrated among different bamboo distribution regions. Giant pandas were mainly distributed in high-altitude areas that had secondary forest, tall trees with a large diameter at breast height (DBH) and bamboo forest. The spatial distribution pattern and microhabitat selection of giant pandas in Meigu Dafengding National Nature Reserve were determined. The findings of this study provide scientific implications for the local conservation and management of the habitat of wild giant pandas.
Genome-wide identification of GAD family genes suggests GhGAD6 functionally respond to Cd2+ stress in cotton
Authors: Huang Hui , He Yunxin , Cui Aihua , Sun Liangqing , Han Mingge , Wang Jing , Rui Cun , Lei Yuqian , Liu Xiaoyu , Xu Nan , Zhang Hong , Zhang Yuexin , Fan Yapeng , Feng Xixian , Ni Kesong , Jiang Jie , Zhang Xingping , Chen Chao , Wang Shuai , Chen Xiugui , Lu Xuke , Wang Delong , Wang Junjuan , Yin Zujun , Qaraevna Bobokhonova Zebinisso , Guo Lixue , Zhao Lanjie , Ye Wuwei
Published: September 2022

Glutamate decarboxylase (GAD) mainly regulated the biosynthesis of γ-aminobutyric acid (GABA) and played an important role in plant growth and stress resistance. To explore the potential function of GAD in cotton growth, the genome-wide identification, structure, and expression analysis of GAD genes were performed in this study. There were 10, 9, 5, and 5 GAD genes identified in G. hirsutum, G. barbadense, G. arboreum, and G. raimondii, respectively. GAD was divided into four clades according to the protein motif composition, gene structure, and phylogenetic relationship. The segmental duplication was the main way of the GAD gene family evolution. Most GhGADs respond to abiotic stress. Clade Ⅲ GAD was induced by Cd2+ stress, especially GhGAD6, and silencing GhGAD6 would lead to more serious Cd2+ poisoning in cotton. The oxidative damage caused by Cd2+ stress was relieved by increasing the GABA content. It was speculated that the decreased expression of GhGAD6 reduced the content of GABA in vivo and caused the accumulation of ROS. This study will further expand our understanding of the relationship between the evolution and function of the GhGAD gene family and provide new genetic resources for cotton breeding under environmental stress and phytoremediation.
A clustering-based sampling method for miRNA-disease association prediction
Authors: Wei Zheng , Yao Dengju , Zhan Xiaojuan , Zhang Shuli
Published: September 2022

More and more studies have proved that microRNAs (miRNAs) play a critical role in gene expression regulation, and the irregular expression of miRNAs tends to be associated with a variety of complex human diseases. Because of the high cost and low efficiency of identifying disease-associated miRNAs through biological experiments, scholars have focused on predicting potential disease-associated miRNAs by computational methods. Considering that the existing methods are flawed in constructing negative sample set, we proposed a clustering-based sampling method for miRNA-disease association prediction (CSMDA). Firstly, we integrated multiple similarity information of miRNA and disease to represent miRNA-disease pairs. Secondly, we performed a clustering-based sampling method to avoid introducing potential positive samples when constructing negative sample set. Thirdly, we employed a random forest-based feature selection method to reduce noise and redundant information in the high-dimensional feature space. Finally, we implemented an ensemble learning framework for predicting miRNA-disease associations by soft voting. The Precision, Recall, F1-score, AUROC and AUPR of the CSMDA achieved 0.9676, 0.9545, 0.9610, 0.9928, and 0.9940, respectively, under five-fold cross-validation. Besides, case study on three cancers showed that the top 20 potentially associated miRNAs predicted by the CSMDA were confirmed by the dbDEMC database or literatures. The above results demonstrate that the CSMDA can predict potential disease-associated miRNAs more accurately.
Construction of a focal adhesion signaling pathway-related ceRNA network in pelvic organ prolapse by transcriptome analysis
Authors: Yu Xia , He Li , Chen Ying , Lin Wenyi , Liu Hong , Yang Xiu , Ye Ying , Zheng Xuemei , Yang Zhenglin , Lin Yonghong
Published: September 2022

Objective: Pelvic organ prolapse (POP) affects a large proportion of adult women, but the pathogenesis of POP remains unclear. The increase in global population aging will impose a substantial medical burden. Herein, we aimed to explore the related RNAs regulating the occurrence of POP and provide potential therapeutic targets.

Method: Tissue biopsies were collected from the anterior vaginal wall of six women with POP and six matched subjects without POP. The profiles of mRNAs, circRNAs, lncRNAs, and miRNAs were obtained by whole transcriptome RNA sequencing.

Result: The findings revealed that 71 circRNAs, 76 known lncRNAs, 84 miRNAs, and 931 mRNAs were significantly altered (p < 0.05 and |log2FC| > 1). GO and KEGG enrichment analyses indicated that the differentially expressed genes (DEGs) were mainly enriched in the focal adhesion signaling pathway. FLT, ITGA9, VEGFD, PPP1R12B, and ROCK2 were identified as focal adhesion signaling pathway-related hub genes by protein–protein interaction network analysis. Based on the relationships between the DEGs and miRNA, lncRNA and circRNA targets, we constructed a focal adhesion signaling pathway-related ceRNA network. The ceRNA network includes hsa_circ_0002190/hsa_circ_0046843/lnc-CARMN -miR-23a-3p - ROCK2 and hsa_circ_0001326/hsa_circ_0007733/lnc-AC107959/lnc-TPM1-AS - miR-205-5p - ROCK2/PPP1R12B/VEGFD. Moreover, abnormalities in the cytoskeleton in fibroblasts from individuals with POP were observed.

Conclusion: In this study, a focal adhesion signaling pathway-related ceRNA network was constructed, and this network may serve as a target for finding suitable drugs for the treatment of POP.
T cell responses to control fungal infection in an immunological memory lens
Authors: Sharma Jaishree , Mudalagiriyappa Srinivasu , Nanjappa Som Gowda
Published: September 2022

In recent years, fungal vaccine research emanated significant findings in the field of antifungal T-cell immunity. The generation of effector T cells is essential to combat many mucosal and systemic fungal infections. The development of antifungal memory T cells is integral for controlling or preventing fungal infections, and understanding the factors, regulators, and modifiers that dictate the generation of such T cells is necessary. Despite the deficiency in the clear understanding of antifungal memory T-cell longevity and attributes, in this review, we will compile some of the existing literature on antifungal T-cell immunity in the context of memory T-cell development against fungal infections.
Hyperuricemia contributes to glucose intolerance of hepatic inflammatory macrophages and impairs the insulin signaling pathway via IRS2-proteasome degradation
Authors: Zhao Hairong , Lu Jiaming , He Furong , Wang Mei , Yan Yunbo , Chen Binyang , Xie De , Xu Chenxi , Wang Qiang , Liu Weidong , Yu Wei , Xi Yuemei , Yu Linqian , Yamamoto Tetsuya , Koyama Hidenori , Wang Wei , Zhang Chenggui , Cheng Jidong
Published: September 2022

Aim

Numerous reports have demonstrated the key importance of macrophage-elicited metabolic inflammation in insulin resistance (IR). Our previous studies confirmed that hyperuricemia or high uric acid (HUA) treatment induced an IR state in several peripheral tissues to promote the development of type 2 diabetes mellitus (T2DM). However, the effect of HUA on glucose uptake and the insulin sensitivity of macrophages and its mechanism is unclear.

Methods

To assess systemic IR, we generated hyperuricemic mice by urate oxidase knockout (UOX-KO). Then, glucose/insulin tolerance, the tissue uptake of 18F-fluorodeoxyglucose, body composition, and energy balance were assessed. Glucose uptake of circulating infiltrated macrophages in the liver was evaluated by glucose transporter type 4 (GLUT-4) staining. Insulin sensitivity and the insulin signaling pathway of macrophages were demonstrated using the 2-NBDG kit, immunoblotting, and immunofluorescence assays. The immunoprecipitation assay and LC-MS analysis were used to determine insulin receptor substrate 2 (IRS2) levels and its interacting protein enrichment under HUA conditions.

Results

Compared to WT mice (10 weeks old), serum uric acid levels were higher in UOX-KO mice (WT, 182.3 ± 5.091 μM versus KO, 421.9 ± 45.47 μM). Hyperuricemic mice with metabolic disorders and systemic IR showed inflammatory macrophage recruitment and increased levels of circulating proinflammatory cytokines. HUA inhibited the nuclear translocation of GLUT-4 in hepatic macrophages, restrained insulin-induced glucose uptake and glucose tolerance, and blocked insulin IRS2/PI3K/AKT signaling. Meanwhile, HUA mediated the IRS2 protein degradation pathway and activated AMPK/mTOR in macrophages. LC-MS analysis showed that ubiquitination degradation could be involved in IRS2 and its interacting proteins to contribute to IR under HUA conditions.

Conclusion

The data suggest that HUA-induced glucose intolerance in hepatic macrophages contributed to insulin resistance and impaired the insulin signaling pathway via IRS2-proteasome degradation
Novel macrophage-related gene prognostic index for glioblastoma associated with M2 macrophages and T cell dysfunction
Authors: Ji Hang , Liu Zhihui , Wang Fang , Sun Haogeng , Wang Nan , Liu Yi , Hu Shaoshan , You Chao
Published: September 2022

This study aims to construct a Macrophage-Related Gene Prognostic Index (MRGPI) for glioblastoma (GBM) and explore the underlying molecular, metabolic, and immunological features. Based on the GBM dataset from The Cancer Genome Atlas (n = 156), 13 macrophage-related hub genes were identified by weighted gene co-expression network (WGCNA) analysis. 5 prognostic genes screened by Kaplan-Meire (K-M) analysis and Cox regression model were used to construct the MRGPI, including GPR84, NCF2, HK3, LILRB2, and CCL18. Multivariate Cox regression analysis found that the MRGPI was an independent risk factor (HR = 2.81, CI95: 1.13-6.98, p = 0.026), leading to an unfavorable outcome for the MRGPI-high group, which was further validated by 4 validation GBM cohorts (n = 728). Thereafter, the molecular, metabolic, and immune features and the clinical implications of the MRGPI-based groups were comprehensively characterized. Gene set enrichment analysis (GSEA) found that immune-related pathways, including inflammatory and adaptive immune response, and activated eicosanoid metabolic pathways were enriched in the MRGPI-high group. Besides, genes constituting the MRGPI was primarily expressed by monocytes and macrophages at single-cell scope and was associated with the alternative activation of macrophages. Moreover, correlation analysis and receiver operating characteristic (ROC) curves revealed the relevance between the MRGPI with the expression of immune checkpoints and T cell dysfunction. Thus, the responsiveness of samples in the MRGPI-high group to immune checkpoint inhibitors (ICI) was detected by algorithms, including Tumor Immune Dysfunction and Exclusion (TIDE) and Submap. In contrast, the MRGPI-low group had favorable outcome, was less immune active and insensitive to ICI. Together, we have developed a promising biomarker to classify the prognosis, metabolic and immune features for GBM, and provide references for facilitating the personalized application of ICI in GBM.
A radiogenomics biomarker based on immunological heterogeneity for non-invasive prognosis of renal clear cell carcinoma
Authors: Gao Jiahao , Ye Fangdie , Han Fang , Jiang Haowen , Zhang Jiawen
Published: September 2022

Background

Tumor immunological heterogeneity potentially influences the prognostic disparities among patients with clear cell renal cell carcinoma (ccRCC); however, there is a lack of macroscopic imaging tools that can be used to predict immune-related gene expression in ccRCC.

Methods

A novel non-invasive radiogenomics biomarker was constructed for immune-related gene expression in ccRCC. First, 520 ccRCC transcriptomic datasets from The Cancer Genome Atlas (TCGA) were analyzed using a non-negative matrix decomposition (NMF) clustering to identify immune-related molecular subtypes. Immune-related prognostic genes were analyzed through Cox regression and Gene Set Enrichment Analysis (GSEA). We then built a risk model based on an immune-related gene subset to predict prognosis in patients with ccRCC. CT images corresponding to the ccRCC patients in The Cancer Imaging Archive (TCIA) database were used to extract radiomic features. To stratify immune-related gene expression levels, extracted radiogenomics features were identified according to standard consecutive steps. A nomogram was built to combine radiogenomics and clinicopathological information through multivariate logistic regression to further enhance the radiogenomics model. Mann–Whitney U test and ROC curves were used to assess the effectiveness of the radiogenomics marker.

Results

NMF methods successfully clustered patients into diverse subtypes according to gene expression levels in the tumor microenvironment (TME). The relative abundance of 10 immune cell populations in each tissue was also analyzed. The immune-related genomic signature (consisting of eight genes) of the tumor was shown to be significantly associated with survival in patients with ccRCC in TCGA database. The immune-related genomic signature was delineated by grouping the signature expression as either low- or high-risk. Using TCIA database, we constructed a radiogenomics biomarker consisting of 11 radiomic features that were optimal predictors of immune-related gene signature expression levels, which demonstrated AUC (area under the ROC curve) values of 0.76 and 0.72 in the training and validation groups, respectively. The nomogram built by combining radiomics and clinical pathological information could further improve the predictive efficacy of the radiogenomics model (AUC = 0.81, 074).

Conclusions

The novel prognostic radiogenomics biomarker achieved excellent correlation with the immune-related gene expression status of patients with ccRCC and could successfully stratify the survival status of patients in TCGA database. It is anticipated that this work will assist in selecting precise clinical treatment strategies. This study may also lead to precise theranostics for patients with ccRCC in the future.
Capillary leak syndrome following COVID-19 vaccination: Data from the European pharmacovigilance database Eudravigilance
Authors: Ruggiero Rosanna , Balzano Nunzia , Di Napoli Raffaella , Mascolo Annamaria , Berrino Pasquale Maria , Rafaniello Concetta , Sportiello Liberata , Rossi Francesco , Capuano Annalisa
Published: September 2022

Capillary leak syndrome (CLS) emerged as new adverse event after immunization (AEFI) associated to COVID-19 vaccination. CLS is a rare condition characterized by increased capillary permeability, resulting in hypoalbuminemia, hypotension, and edema mainly in the upper and lower limbs. Our pharmacovigilance study aims to evaluate the CLS onset following receipt of COVID-19 mRNA vaccines (mRNA-1273 and BNT162b2) compared to viral vector vaccines (Ad26.COV2-S and ChAdOx1-SARS-COV-2). We carried a cross-sectional study using all Individual Case Safety Reports (ICSRs) reporting a COVID-19 vaccine as suspected drug and CLS as AEFI, which were collected in the pharmacovigilance database EudraVigilance from January 1st, 2021, to January 14th, 2022. We applied the Reporting Odds Ratio (ROR) 95% CI for the disproportionality analysis. During our study period, CLS was described as AEFI in 84 out of 1,357,962 ICRs reporting a vaccine COVID-19 as suspected drug and collected in the EV database. Overall, the ICSR reported by CLS were mainly related to the viral vector COVID-19(ChAdOx1-SARS-COV-2 = 36; Ad26.COV2-S = 9). The mRNA COVID-19 vaccines were reported in 39 ICSRs (BNT162b2 =33; mRNA-1273 =6). Majority of ICSRs were reported by healthcare professionals (71.4%). Majority of the patients were adult (58.3%) and the female gender accounted in more than 65% of ICSRs referred both to classes vaccines. In particular, women were more represented in ICSRs referred to mRNA-1273 (83.3%) and to ChAdOx1-SARS-COV-2 (72.2%). The CLS outcome was more frequently favorable in mRNA ICSRs (33,3%) than the viral vector ones (13.3%). Among the ICSRs reporting CLS with unfavorable outcome, we found also 9 fatal cases (BNT162b2 = 1; ChAdOx1-SARS-COV-2 = 4; Ad26.COV2-S = 4). From disproportionality analysis emerged a lower CLS reporting probability after vaccination with mRNA vaccines compared to viral vector-based ones (ROR 0.5, 95% CI 0.3–0.7; p <0.001).Our findings, even if subject to the limitations of spontaneous reporting systems, suggest a small but statistically significant safety concern for CLS following receipt of COVID-19 viral vector vaccines, in particular with Ad26.COV2-S. Cytokine-release following T-cell activation could be involved in CLS occurrence, but a precise mechanism has been not yet identified. COVID-19 vaccines remain attentive as possible triggers of CLS.
Composition and regulation of the immune microenvironment of salivary gland in Sjögren’s syndrome
Authors: Tan Zhen , Wang Li , Li Xiaomei
Published: September 2022

Primary Sjögren’s syndrome (pSS) is a systemic autoimmune disease characterized by exocrine gland dysfunction and inflammation. Patients often have dry mouth and dry eye symptoms, which seriously affect their lives. Improving dry mouth and eye symptoms has become a common demand from patients. For this reason, researchers have conducted many studies on external secretory glands. In this paper, we summarize recent studies on the salivary glands of pSS patients from the perspective of the immune microenvironment. These studies showed that hypoxia, senescence, and chronic inflammation are the essential characteristics of the salivary gland immune microenvironment. In the SG of pSS, genes related to lymphocyte chemotaxis, antigen presentation, and lymphocyte activation are upregulated. Interferon (IFN)-related genes, DNA methylation, sRNA downregulation, and mitochondrial-related differentially expressed genes are also involved in forming the immune microenvironment of pSS, while multiple signaling pathways are involved in regulation. We further elucidated the regulation of the salivary gland immune microenvironment in pSS and relevant, targeted treatments.
Refined polysaccharide from Dendrobium devonianum resists H1N1 influenza viral infection in mice by activating immunity through the TLR4/MyD88/NF-κB pathway
Authors: Wei Xueping , Sun Wei , Zhu Pengpeng , Ou Guoteng , Zhang Sheng , Li Yuanyuan , Hu Jingjin , Qu Xuefeng , Zhong Yan , Yu Wenying , You Zhenqiang , Wang Yin , Wu Yueguo
Published: September 2022

Dendrobium polysaccharide exhibits multiple biological activities, such as immune regulation, antioxidation, and antitumor. However, its resistance to viral infection by stimulating immunity is rarely reported. In this study, we explored the effect and mechanism of DVP-1, a novel polysaccharide from Dendrobium devonianum, in the activation of immunity. After being activated by DVP-1, the ability of mice to prevent H1N1 influenza virus infection was investigated. Results of immune regulation showed that DVP-1 significantly improved the immune organ index, lymphocyte proliferation, and mRNA expression level of cytokines, such as IL-1β, IL-4, IL-6, and TNF-α in the spleen. Immunohistochemical results showed that DVP-1 obviously promoted the mucosal immunity in the jejunum tissue. In addition, the expression levels of TLR4, MyD88, and TRAF6 and the phosphorylation levels of TAK1, Erk, JNK, and NF-κB in the spleen were upregulated by DVP-1. The virus infection results showed that the weight loss of mice slowed down, the survival rate increased, the organ index of the lung reduced, and the virus content in the lung decreased after DVP-1 activated immunity. By activating immunity with DVP-1, the production of inflammatory cells and inflammatory factors in BALF, and alveolar as well as peribronchiolar inflammation could be prevented. The results manifested that DVP-1 could resist H1N1 influenza virus infection by activating immunity through the TLR4/MyD88/NF-κB pathway.