bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of an AAV9-RNAi-mediated silencing strategy to abrogate TRPM4 expression in the adult heart
Authors: Medert Rebekka , Jungmann Andreas , Hildebrand Staffan , Busch Martin , Grimm Dirk , Flockerzi Veit , Müller Oliver J. , Most Patrick , Schumacher Dagmar , Freichel Marc
Published: February 2021

The cation channel transient receptor potential melastatin 4 (TRPM4) is a calcium-activated non-selective cation channel and acts in cardiomyocytes as a negative modulator of the L-type Ca2+ influx. Global deletion of TRPM4 in the mouse led to increased cardiac contractility under β-adrenergic stimulation. Consequently, cardiomyocyte-specific inactivation of the TRPM4 function appears to be a promising strategy to improve cardiac contractility in heart failure patients. The aim of this study was to develop a gene therapy approach in mice that specifically silences the expression of TRPM4 in cardiomyocytes. First, short hairpin RNAmiR30 (shRNAmiR30) sequences against the TRPM4 mRNA were screened in vitro using lentiviral transduction for a stable expression of the shRNA cassettes. Western blot analysis identified three efficient shRNAmiR30 sequences out of six, which reduced the endogenous TRPM4 protein level by up to 90 ± 6%. Subsequently, the most efficient shRNAmiR30 sequences were delivered into cardiomyocytes of adult mice using adeno-associated virus serotype 9 (AAV9)-mediated gene transfer. Initially, the AAV9 vector particles were administered via the lateral tail vein, which resulted in a downregulation of TRPM4 by 46 ± 2%. Next, various optimization steps were carried out to improve knockdown efficiency in vivo. First, the design of the expression cassette was streamlined for integration in a self-complementary AAV vector backbone for a faster expression. Compared to the application via the lateral tail vein, intravenous application via the retro-orbital sinus has the advantage that the vector solution reaches the heart directly and in a high concentration, and eventually a TRPM4 knockdown efficiency of 90 ± 7% in the heart was accomplished by this approach. By optimization of the shRNAmiR30 constructs and expression cassette as well as the route of AAV9 vector application, a 90% reduction of TRPM4 expression was achieved in the adult mouse heart. In the future, AAV9-RNAi-mediated inactivation of TRPM4 could be a promising strategy to increase cardiac contractility in preclinical animal models of acute and chronic forms of cardiac contractile failure.
Correction to: Pediatric procedural sedation and analgesia in the emergency department: surveying the current European practice
Authors: Sahyoun Cyril , Cantais Aymeric , Gervaix Alain , Bressan Silvia , Löllgen Ruth , Krauss Baruch ,   on behalf of the Pediatric Emergency Medicine Comfort and Analgesia Research in Europe (PemCARE) group of the Research in European Pediatric Emergency Medicine (REPEM) network, de Jaeger Annick , Frederiksen Marianne Sjølin , Chéron Gérard , Röher Katharina , Hoffmann Florian , Fodor László , Sforzi Idanna , Shavit Itai , Pucuka Zanda , Masilionis Vytenis , Farrugia Ruth , Borensztajn Dorine , Garrido Ana , Moldovan Diana , Navarro Maria-Concepcion Miguez , Orfanos Ioannis , Er Anil , Duman Murat
Published: February 2021

A correction to this paper has been published: 10.1007/s00431-021-03980-w
Post-magmatic fracturing, fluid flow, and vein mineralization in supra-subduction zones: a comparative study on vein calcites from the Troodos ophiolite and the Izu–Bonin forearc and rear arc
Authors: Quandt Dennis , Kurz W. , Micheuz P.
Published: February 2021

Based on the published data of pillow lava-hosted mineralized veins, this study compares post-magmatic fracturing, fluid flow, and secondary mineralization processes in the Troodos and Izu–Bonin supra-subduction zone (SSZ) and discusses the crucial factors for the development of distinct vein types. Thin section and cathodoluminescence petrography, Raman spectroscopy, fluid inclusion microthermometry, and trace element and isotope (87Sr/86Sr, δ18O, δ13C, Δ47) geochemistry indicate that most veins consist of calcite that precipitated from pristine to slightly modified seawater at temperatures < 50 °C. In response to the mode of fracturing, fluid supply, and mineral growth dynamics, calcites developed distinct blocky (precipitation into fluid-filled fractures), syntaxial (crack and sealing), and antitaxial (diffusion-fed displacive growth) vein microtextures with vein type-specific geochemical signatures. Blocky veins predominate in all study areas, whereas syntaxial veins represent subordinate structures. Antitaxial veins occur in all study areas but are particularly abundant in the Izu–Bonin rear arc where the local geological setting was conducive of antitaxial veining. The temporal framework of major calcite veining coincides with the onset of extensional faulting in the respective areas and points to a tectonic control on veining. Thus, major calcite veining in the Troodos SSZ began contemporaneously with volcanic activity and extensional faulting and completed within ~ 10–20 Myr. This enabled deep seawater downflow and hydrothermal fluid upflow. In the Izu–Bonin forearc, reliable ages of vein calcites point to vein formation > 15 Myr after subduction initiation. Therefore, high-T mineralization (calcite, quartz, analcime) up to 230 °C is restricted to the Troodos SSZ.
Identification and genotyping of a new subtype of bovine viral diarrhea virus 1 isolated from cattle with diarrhea
Authors: Tian Bin , Cai Dongjie , Li Weiqiang , Bu Qinglong , Wang Mingshu , Ye Gang , Liu Jianzhu , Wang Ya , Gou Liping , Yi Jun , Zuo Zhicai
Published: February 2021

In 2019, diarrhea cases occurred on cattle farms in Qionglai and Guang'an, Sichuan Province. Two out of 20 (10%) serum and nasal swab samples were positive when tested using a bovine viral diarrhea virus (BVDV) antigen-capture ELISA kit. Two non-cytopathic strains of BVDV were isolated and named QL1903 and GA190608, respectively. The nucleotide sequences of the genomes of the two isolates were 89.52% identical. Phylogenetic analysis based on the 5'-UTR sequence revealed that the BVDV isolate QL1903 belonged to BVDV subtype 1b, whereas isolate GA190608 clustered with strains HN1814, EN-19, and BJ09_26 in a separate branch, which has tentatively been classified as a new genetic subtype, "1v".
Paleoproteomic profiling of organic residues on prehistoric pottery from Malta
Authors: Tanasi Davide , Cucina Annamaria , Cunsolo Vincenzo , Saletti Rosaria , Di Francesco Antonella , Greco Enrico , Foti Salvatore
Published: February 2021

Mass spectrometry-based approaches have been successfully applied for identifying ancient proteins in bones and other tissues. On the contrary, there are relatively few examples of the successful recovery and identification of archeological protein residues from ceramic artifacts; this is because ceramics contain much lower levels of proteins which are extensively degraded by diagenetic effects. In this paper, we report the results of the characterization of proteins extracted from pottery of the Maltese site of Baħrija, the guide-site for the Baħrija period (half of 9th–second half of eighth century BCE), recently identified as the final part of the Borġ in-Nadur culture. Proteomic data here reported confirm that one of the major issue of these kind of studies is represented by contamination of animal and human agents that may complicate endogenous protein identification and authentication. The samples tested included a small group of ceramic forms, namely three tableware and six coarse ware thought to have been used in food preparation and/or storage. In this context, the limited availability of paleobotanical and archeozoological analyses may be compensated by the outcomes of the first proteomics profiling which, even if obtained on a limited selection of vessels, revealed the centrality of wheat in the diet of the ancient community of Baħrija. The data have been deposited to the ProteomeXchange with identifier < PXD022848 > .
Cache-efficient sweeping-based interval joins for extended Allen relation predicates
Authors: Piatov Danila , Helmer Sven , Dignös Anton , Persia Fabio
Published: February 2021

We develop a family of efficient plane-sweeping interval join algorithms for evaluating a wide range of interval predicates such as Allen’s relationships and parameterized relationships. Our technique is based on a framework, components of which can be flexibly combined in different manners to support the required interval relation. In temporal databases, our algorithms can exploit a well-known and flexible access method, the Timeline Index, thus expanding the set of operations it supports even further. Additionally, employing a compact data structure, the gapless hash map, we utilize the CPU cache efficiently. In an experimental evaluation, we show that our approach is several times faster and scales better than state-of-the-art techniques, while being much better suited for real-time event processing.
Distribution of non-spherical nanoparticles in turbulent flow of ventilation chamber considering fluctuating particle number density
Authors: Shi Ruifang , Lin Jianzhong , Yang Hailin , Yu Mingzhou
Published: February 2021

The Reynolds-averaged general dynamic equation (RAGDE) for the nanoparticle size distribution function is derived, including the contribution to particle coagulation resulting from the fluctuating concentration. The equation together with that of a turbulent gas flow is solved numerically in the turbulent flow of a ventilation chamber with a jet on the wall based on the proposed model relating the fluctuating coagulation to the gradient of mean concentration. Some results are compared with the experimental data. The results show that the proposed model relating the fluctuating coagulation to the gradient of mean concentration is reasonable, and it is necessary to consider the contribution to coagulation resulting from the fluctuating concentration in such a flow. The changes of the particle number concentration M0 and the geometric mean diameter dg are more obvious in the core area of the jet, but less obvious in other areas. With the increase in the initial particle number concentration m00, the values of M0 and the standard deviation of the particle size σ decrease, but the value of dg increases. The decrease in the initial particle diameter leads to the reduction of M0 and σ, and the increase in dg. With the increase in the Reynolds number, particles have few chances of collision, and hence the coagulation rate is reduced, leading to the increase in M0 and σ, and the decrease in dg.
A Taxonomy of Noncanonical Uses of Interrogatives
Authors: Puczyłowski Tomasz
Published: February 2021

The aims of this paper are (i) to provide a detailed taxonomy of noncanonical uses of interrogative sentences, i.e. when they are used not to ask a question but to convey some information, or to ask a question albeit not that expressed by the interrogative sentence exploited in the act, (ii) to identify properties of circumstances where an interrogative sentence is being used in this way, and (iii) to propose some maxims that govern the rational use of questions. Four main categories of such cases are presented, and a few further subclasses are differentiated. I show how these types are interrelated, and what logical features differentiate them. I also propose a hypothesis for when an interrogative sentence is not being used in its primary mode. Studies on circumstances in which questions are used in other ways can shed light on maxims that govern asking and questioning in a rational conversation; therefore, some possible maxims of this kind are proposed.
Genetic Influences on the Covariance and Genetic Correlations in a Bivariate Twin Model: An Application to Well-Being
Authors: de Vries Lianne P. , van Beijsterveldt Toos C. E. M. , Maes Hermine , Colodro-Conde Lucía , Bartels Meike
Published: February 2021

The distinction between genetic influences on the covariance (or bivariate heritability) and genetic correlations in bivariate twin models is often not well-understood or only one is reported while the results show distinctive information about the relation between traits. We applied bivariate twin models in a large sample of adolescent twins, to disentangle the association between well-being (WB) and four complex traits (optimism, anxious-depressed symptoms (AD), aggressive behaviour (AGG), and educational achievement (EA)). Optimism and AD showed respectively a strong positive and negative phenotypic correlation with WB, the negative correlation of WB and AGG is lower and the correlation with EA is nearly zero. All four traits showed a large genetic contribution to the covariance with well-being. The genetic correlations of well-being with optimism and AD are strong and smaller for AGG and EA. We used the results of the models to explain what information is retrieved based on the bivariate heritability versus the genetic correlations and the (clinical) implications.
Using indicator species to detect high quality habitats in an East African forest biodiversity hotspot
Authors: Habel Jan Christian , Zamora Camilo , Rödder Dennis , Teucher Mike , Cuadros-Casanova Ivon , Fischer Christina
Published: February 2021

Species demanding specific habitat requirements suffer, particularly under environmental changes. The smallest owl of Africa, the Sokoke Scops Owl (Otus ireneae), occurs exclusively in East African coastal forests. To understand the movement behaviour and habitat demands of O. ireneae, we combined data from radio-tracking and remote sensing to calculate Species Distribution Models across the Arabuko Sokoke forest in southern Kenya. Based on these data, we estimated the local population size and projected the distribution of current suitable habitats. We found that the species occurs only in Cynometra woodland with large old trees and dense vegetation. Based on home range sizes and the distribution of suitable forest habitats, the local population size was estimated at < 400 pairs. Ongoing selective logging of hard-wood trees and the production of charcoal are reducing habitat quality of which will reduce the low numbers of O. ireneae, and of other specialist forest species, even further. Due to their close connection with intact Cynometra forest, O. ireneae is an excellent indicator of intact forest remnants. In addition, this species is a suitable flagship for the promotion and conservation of the last remaining coastal forests of East Africa.
The Natura 2000 network and the ranges of threatened species in Greece
Authors: Spiliopoulou Konstantina , Dimitrakopoulos Panayiotis G. , Brooks Thomas M. , Kelaidi Gabriela , Paragamian Kaloust , Kati Vassiliki , Oikonomou Anthi , Vavylis Dimitris , Trigas Panayiotis , Lymberakis Petros , Darwall William , Stoumboudi Maria Th. , Triantis Kostas A.
Published: February 2021

Global environmental goals mandate the expansion of the protected area network to halt biodiversity loss. The European Union’s Natura 2000 network covers 27.3% of the terrestrial area of Greece, one of the highest percentages in Europe. However, the extent to which this network protects Europe’s biodiversity, especially in a biodiverse country like Greece, is unknown. Here, we overlap the country’s Natura 2000 network with the ranges of the 424 species assessed as threatened on the IUCN Red List and present in Greece. Natura 2000 overlaps on average 47.6% of the mapped range of threatened species; this overlap far exceeds that expected by random networks (21.4%). Special Protection Areas and Special Areas of Conservation (non-exclusive subsets of Natura 2000 sites) overlap 33.4% and 38.1% respectively. Crete and Peloponnese are the two regions with the highest percentage of threatened species, with Natura 2000 sites overlapping on average 62.3% with the threatened species’ ranges for the former, but only 30.6% for the latter. The Greek ranges of all 62 threatened species listed in Annexes 1 and II to the Birds and Habitats Directives are at least partially overlapped by the network (52.0%), and 18.0% of these are fully overlapped. However, the ranges of 27 threatened species, all of which are endemic to Greece, are not overlapped at all. These results can inform national policies for the protection of biodiversity beyond current Natura 2000 sites.
Dissolved organic nutrients at the interface of fresh and marine waters: flow regime changes, biogeochemical cascades and picocyanobacterial blooms—the example of Florida Bay, USA
Authors: Glibert Patricia M. , Heil Cynthia A. , Madden Christopher J. , Kelly Stephen P.
Published: February 2021

The availability of dissolved inorganic and organic nutrients and their transformations along the fresh to marine continuum are being modified by various natural and anthropogenic activities and climate-related changes. Subtropical central and eastern Florida Bay, located at the southern end of the Florida peninsula, is classically considered to have inorganic nutrient conditions that are in higher-than-Redfield ratio proportions, and high levels of organic and chemically-reduced forms of nitrogen. However, salinity, pH and nutrients, both organic and inorganic, change with changes in freshwater flows to the bay. Here, using a time series of water quality and physico-chemical conditions from 2009 to 2019, the impacts of distinct changes in managed flow, drought, El Niño-related increases in precipitation, and intensive storms and hurricanes are explored with respect to changes in water quality and resulting ecosystem effects, with a focus on understanding why picocyanobacterial blooms formed when they did. Drought produced hyper-salinity conditions that were associated with a seagrass die-off. Years later, increases in precipitation resulting from intensive storms and a hurricane were associated with high loads of organic nutrients, and declines in pH, likely due to high organic acid input and decaying organic matter, collectively leading to physiologically favorable conditions for growth of the picocyanobacterium,Synechococcusspp. These conditions, including very high concentrations of NH4+, were likely inhibiting for seagrass recovery and for growth of competing phytoplankton or their grazers. Given projected future climate conditions, and anticipated cycles of drought and intensive storms, the likelihood of future seagrass die-offs and picocyanobacterial blooms is high.
Differences and similarities between kraft and oxygen delignification of softwood fibers: effects on chemical and physical properties
Authors: Esteves Cláudia V. , Sevastyanova Olena , Östlund Sören , Brännvall Elisabet
Published: February 2021

The fiber properties after oxygen delignification and kraft pulping were studied by looking into the chemical characteristics and morphology. The effect of the two processes on the fibers was evaluated and compared over a wider kappa number range (from 62 down to15). Wide-angle X-ray scattering, nuclear magnetic resonance and fiber saturation point were used to characterize the fiber network structure. Fiber morphology and fiber dislocations were evaluated by an optical image analysis. The total and surface fiber charges were studied by conductometric and polyelectrolyte titrations. The fiber wall supramolecular structure, such as crystallinity, size of fibril aggregates, pore size and pore volume, were similar for the two processes. The selectivity, in terms of carbohydrate yield, was equal for kraft cooking and oxygen delignification, but the selectivity in terms of viscosity loss per amount of delignification is poorer for oxygen delignification. Clearly more fiber deformations (2–6% units in curl index) in the fibers after oxygen delignification were seen. Introduction of curl depended on the physical state of the fibers, i.e. liberated or in wood matrix. In the pulping stage, the fiber continue to be supported by neighboring fibers, as the delignified chips maintain their form. However, in the subsequent oxygen stage the fibers enter in the form of pulp (liberated fibers), which makes them more susceptible to changes in fiber form.

Graphic abstract
The extent of livelihood diversification on the determinants of livelihood diversification in Assosa Wereda, Western Ethiopia
Authors: Ayana Geleta Fekadu , Megento Tebarek Lika , Kussa Fekadu Gurmessa
Published: February 2021

This study was conducted to examine the determinants of diversification of livelihoods to investigate the degree of diversification of households’ livelihoods in Assosa wereda, Benshangul Gumuz Regional State, in western Ethiopia. A total of 320 sample rural households were selected by using systematic sampling technique from four kebeles. Both qualitative and quantitative data collected through a structured questionnaire, key informant interview, focus group discussions and observation. The data were analyzed using descriptive statistical tools, the multinomial logit regression model, and Simpson Diversification Index. The result of the study show, of the 14 determinants of diversification of livelihoods identified: level of education, dependency ratio, access to irrigation, and household urban linkage were significant predictors of diversification of livelihoods. Whereas household age and sex, road distance, credit access, and household media access were insignificant predictors of diversification of livelihoods. Meanwhile, land hold size, livestock holding size in Tropical Livestock Unit, extension contact, cooperative member and total household income couldn’t have any association with household diversification of livelihoods in real conditions on the ground. Ultimately, the Simpson Diversity Index result showed that there was no diversification rather than "specialization" in livelihood. The study therefore urges more diversification opportunities in poverty alleviation and development.
Effect of chemical modification on electronic transport properties of carbyne
Authors: Berdiyorov G. R. , Khalilov U. , Hamoudi H. , Neyts Erik C.
Published: February 2021

Using density functional theory in combination with the Green’s functional formalism, we study the effect of surface functionalization on the electronic transport properties of 1D carbon allotrope—carbyne. We found that both hydrogenation and fluorination result in structural changes and semiconducting to metallic transition. Consequently, the current in the functionalization systems increases significantly due to strong delocalization of electronic states along the carbon chain. We also study the electronic transport in partially hydrogenated carbyne and interface structures consisting of pristine and functionalized carbyne. In the latter case, current rectification is obtained in the system with rectification ratio up to 50%. These findings can be useful for developing carbyne-based structures with tunable electronic transport properties.
Stable Dynamical Adaptive Mesh Refinement
Authors: Lundquist Tomas , Nordström Jan , Malan Arnaud
Published: February 2021

We consider accurate and stable interpolation procedures for numerical simulations utilizing time dependent adaptive meshes. The interpolation of numerical solution values between meshes is considered as a transmission problem with respect to the underlying semi-discretized equations, and a theoretical framework using inner product preserving operators is developed, which allows for both explicit and implicit implementations. The theory is supplemented with numerical experiments demonstrating practical benefits of the new stable framework. For this purpose, new interpolation operators have been designed to be used with multi-block finite difference schemes involving non-collocated, moving interfaces.
Large Time Convergence of the Non-homogeneous Goldstein-Taylor Equation
Authors: Arnold Anton , Einav Amit , Signorello Beatrice , Wöhrer Tobias
Published: February 2021

The Goldstein-Taylor equations can be thought of as a simplified version of a BGK system, where the velocity variable is constricted to a discrete set of values. It is intimately related to turbulent fluid motion and the telegrapher’s equation. A detailed understanding of the large time behaviour of the solutions to these equations has been mostly achieved in the case where the relaxation function, measuring the intensity of the relaxation towards equally distributed velocity densities, is constant. The goal of the presented work is to provide a general method to tackle the question of convergence to equilibrium when the relaxation function is not constant, and to do so as quantitatively as possible. In contrast to the usual modal decomposition of the equations, which is natural when the relaxation function is constant, we define a new Lyapunov functional of pseudodifferential nature, one that is motivated by the modal analysis in the constant case, that is able to deal with full spatial dependency of the relaxation function. The approach we develop is robust enough that one can apply it to multi-velocity Goldstein-Taylor models, and achieve explicit rates of convergence. The convergence rate we find, however, is not optimal, as we show by comparing our result to those found in [8].
Multi-taxon conservation in northern forest hot-spots: the role of forest characteristics and spatial scales
Authors: Kärvemo Simon , Jönsson Mari , Hekkala Anne-Maarit , Sjögren Jörgen , Strengbom Joachim
Published: February 2021

Context

Biodiversity is highly affected by industrial forestry, which leads to the loss and fragmentation of natural habitats. To date, most conservation studies have evaluated associations among a single species group, forest type, or spatial scale.

Objective

The objective was to evaluate the richness of multiple species groups across various forest types and characteristics at multiple scales.

Methods

We used the occurrence data for 277 species of conservation interest from 455 stands of high conservation value, including four species groups and four forest types.

Results

Local, landscape, and regional forest characteristics influenced biodiversity in a non-uniform pattern among species groups and forest types. For example, an increased local spruce basal area in spruce forests was associated with higher vascular plant and bryophyte richness values, whereas macrofungi and lichen richness were positively correlated with deadwood availability, but negatively correlated with the spruce volume in the landscape. Furthermore, landscapes with twice as much mature forest as the average, had more than 50% higher richness values for vascular plants, macrofungi, and lichens.

Conclusion

Among sessile species groups in northern forests, a uniform conservation strategy across forest types and scales is suboptimal. A multi-faceted strategy that acknowledges differences among species groups and forest types with tailored measures to promote richness is likely to be more successful. Nevertheless, the single most common measure associated with high richness across the species groups and forest types was mature forest in the landscape, which suggests that increasing old forests in the landscape is a beneficial conservation strategy.
No magic bullet: estimating anti-immigrant sentiment and social desirability bias with the item-count technique
Authors: Rinken S. , Pasadas-del-Amo S. , Rueda M. , Cobo B.
Published: February 2021

Extant scholarship on attitudes toward immigration and immigrants relies mostly on direct survey items. Thus, little is known about the scope of social desirability bias, and even less about its covariates. In this paper, we use probability-based mixed-modes panel data collected in the Southern Spanish region of Andalusia to estimate anti-immigrant sentiment with both the item-count technique, also known as list experiment, and a direct question. Based on these measures, we gauge the size of social desirability bias, compute predictor models for both estimators of anti-immigrant sentiment, and pinpoint covariates of bias. For most respondent profiles, the item-count technique produces higher estimates of anti-immigrant sentiment than the direct question, suggesting that self-presentational concerns are far more ubiquitous than previously assumed. However, we also find evidence that among people keen to position themselves as all-out xenophiles, social desirability pressures persist in the list-experiment: the full scope of anti-immigrant sentiment remains elusive even in non-obtrusive measurement.
Health-related quality of life and its predictors among adults living with HIV/AIDS and receiving antiretroviral therapy in Pakistan
Authors: Ahmed Ali , Saqlain Muhammad , Bashir Naila , Dujaili Juman , Hashmi Furqan , Mazhar Faizan , Khan Amjad , Jabeen Musarat , Blebil Ali , Awaisu Ahmed
Published: February 2021

Background

Health-related quality of life (HRQoL) is considered to be the fourth 90 of UNAIDS 90-90-90 target to monitor the effects of combination antiretroviral therapy (ART). ART has significantly increased the life expectancy of people living with HIV/AIDS (PLWHA). However, the impact of chronic infection on HRQoL remains unclear, while factors influencing the HRQoL may vary from one country to another. The current study aimed to assess HRQoL and its associated factors among PLWHA receiving ART in Pakistan.

Methods

A cross-sectional descriptive study was conducted among PLWHA attending an ART centre of a tertiary care hospital in Islamabad, Pakistan. HRQoL was assessed using a validated Urdu version of EuroQol 5 dimensions 3 level (EQ-5D-3L) and its Visual Analogue Scale (EQ-VAS).

Results

Of the 602 patients included in the analyses, 59.5% (n = 358) reported no impairment in self-care, while 63.1% (n = 380) were extremely anxious/depressed. The overall mean EQ-5D utility score and visual analogue scale (EQ-VAS) score were 0.388 (SD: 0.41) and 66.20 (SD: 17.22), respectively. Multivariate linear regression analysis revealed that the factors significantly associated with HRQoL were: female gender; age  > 50 years; having primary and secondary education;  > 1 year since HIV diagnosis; HIV serostatus AIDS-converted; higher CD 4 T lymphocytes count; detectable viral load; and increased time to ART.

Conclusions

The current findings have shown that PLWHA in Pakistan adherent to ART had a good overall HRQoL, though with significantly higher depression. Some of the factors identified are amenable to institution-based interventions while mitigating depression to enhance the HRQoL of PLWHA in Pakistan. The HRQoL determined in this study could be useful for future economic evaluation studies for ART and in designing future interventions.
On Muzzles and Faces: The Semiotic Limits of Visage and Personhood
Authors: Leone Massimo
Published: February 2021

The essay investigates the anthropological concept of personhood from the point of view of the dialectics between two fundamental elements of the socio-cultural, linguistic, and semiotic construction of the self-identity of the human species: on the one hand, the human face and, on the other, the non-human muzzle.
After demonstrating that their semantics is contrastively articulated in all Indo-European languages, and after showing that such contrast is featured also in several non-Indo-European languages, including those referring to supposedly alternative “ontologies of nature”, the essay criticizes such opposition through a close reading of Lévinas, Deleuze and Guattari, and Derrida’s philosophical texts on the face and on animality. Ultimately, it proposes that the construction of the animal muzzle as an interface of non-personhood is instrumental to the substitution of the human victim in the sacrifice that establishes the human community. Only through eradicating the primordial stigmatization of the muzzle, however, will a non-violent foundation of human personhood and community be possible.
Use of the VerifyNow point of care assay to assess the pharmacodynamic effects of loading and maintenance dose regimens of prasugrel and ticagrelor
Authors: Angiolillo Dominick J. , Been Latonya , Rubinstein Marc , Martin Michael , Rollini Fabiana , Franchi Francesco
Published: February 2021

Prasugrel and ticagrelor are potent oral platelet P2Y12 inhibitors and are recommended over clopidogrel in patients with acute coronary syndrome (ACS). Oral platelet P2Y12 inhibitors are characterized by varying degrees of pharmacodynamic response profiles as assessed by a variety of commercially available assays. Because of its ease of use, rapid turnaround times and ability to provide results specific to P2Y12 inhibitory effects, VerifyNow has emerged as one of the most commonly utilized platelet function assays. However, reference ranges with VerifyNow have been reported mainly for clopidogrel and there has not yet been any study specifically conducted to provide the expected on treatment reference ranges following administration of prasugrel and ticagrelor. This was a prospective single center investigation conducted in 120 patients with ACS who were treated with prasugrel or ticagrelor as per standard of care. Patients who underwent percutaneous coronary interventions (PCI) were treated with a loading dose of prasugrel (60 mg) or ticagrelor (180 mg), and patients who were on maintenance therapy were taking prasugrel (10 mg qd or 5 mg qd) or ticagrelor (90 mg bid). Platelet function testing was performed using the VerifyNow™ PRUTest™. The overall range of PRUTest values was lower than that observed in studies of patients treated with clopidogrel. The use of a maintenance dose regimen had a wider range of PRUTest values compared to the use of a loading dose for both prasugrel (1–179 vs. 2–128) and ticagrelor (1–196 vs. 1–177). The average PRUTest values in patients on prasugrel and ticagrelor maintenance dosing were 20% and 9% higher those observed in patients treated with a loading dose. PRUTest results following loading dose administration were very similar between drugs, but were 20% higher with prasugrel compared with ticagrelor during maintenance dosing. This study establishes expected PRUTest ranges for patients taking loading and maintenance doses of prasugrel and ticagrelor.

Clinical Trial Registrationhttp://www.clinicaltrials.gov Unique Identifier: NCT04492423, registered July 2020 retrospectively registered.
Thermal Effects on Pull-Off Force in the Johnson–Kendall–Roberts Model
Authors: Zhou Yunong
Published: February 2021

In this study, we investigate the effects of thermal fluctuations on the generalized Johnson–Kendall–Roberts (JKR) model. We show that the distribution of pull-off forces in this model is similar to that of the Bradley model, and is also consistent with the experiment result observed in Wierez-Kien et al. (Nanotechnology 29(15):155704 2018). Increasing temperature leads to a broadening of the distribution, while leads to a reduction of the pull-off force. Additionally, the pull-off force, which is separated into an athermal term and a thermal-induced reduction term, is measured by using spring velocity ranging over 5 orders of magnitude. We show that for compliant spring, the pull-off force is significantly enhanced with increasing velocity, which is mainly attributed to the contribution of the thermal-induced reduction term, while the athermal term is barely sensitive to changes in velocity.
Current Iodine Nutrition Status and Morbidity of Thyroid Nodules in Mainland China in the Past 20 Years
Authors: Liu Xin , Sun Jian , Fang Wei , Xu Yanguo , Zhu Zizhao , Liu Yazhuo
Published: February 2021

The aim of this study was to comprehensively assess the prevalence of goiter and thyroid nodules (TNs) in relation to China’s iodine nutrition level over the past 20 years and provide an effective reference for developing health policies. PubMed, EMBASE, Chinese National Knowledge Infrastructure, Chongqing VIP, and Chinese Wan Fang databases were searched for relevant studies from Jan 1996 to Feb 2020. Two reviewers extracted valid data from the eligible citations to determine the morbidity of TNs in different urinary iodine concentrations (UICs) and in patients of different genders, of different ages, who live in different geographic regions, and who live at different altitudes, as well as the P values of interactions between groups. There were 26 articles (34 studies) included in this analysis. The overall morbidity of TNs in mainland China was 23.4%. Morbidity was higher in urban areas (P < 0.001) than in rural and mixed areas. Coastal areas (P < 0.001), female patients (P < 0.001), high-altitude areas (P < 0.001), and residence in south China (P < 0.001) were all associated with higher morbidity of TNs. The lowest morbidity value of TNs, 16%, was in the more-than-adequate iodine subgroup. The highest morbidity, 27.2%, was in the adequate iodine subgroup. The morbidity of TNs increases with age, and women are more likely to have TNs. We also need to perform more epidemiological studies, and in the future, we should cultivate better understanding of the relationship between other thyroid diseases and provide more comprehensive and useful information for other researchers.
Hippocampal TNF-α Signaling Mediates Heroin Withdrawal-Enhanced Fear Learning and Withdrawal-Induced Weight Loss
Authors: Parekh Shveta V. , Paniccia Jacqueline E. , Adams Lydia O. , Lysle Donald T.
Published: February 2021

There is significant comorbidity of opioid use disorder (OUD) and post-traumatic stress disorder (PTSD) in clinical populations. However, the neurobiological mechanisms underlying the relationship between chronic opioid use and withdrawal and development of PTSD are poorly understood. Our previous work identified that chronic escalating heroin administration and withdrawal can produce enhanced fear learning, an animal model of hyperarousal, and is associated with an increase in dorsal hippocampal (DH) interleukin-1β (IL-1β). However, other cytokines, such as TNF-α, work synergistically with IL-1β and may have a role in the development of enhanced fear learning. Based on both translational rodent and clinical studies, TNF-α has been implicated in hyperarousal states of PTSD, and has an established role in hippocampal-dependent learning and memory. The first set of experiments tested the hypothesis that chronic heroin administration followed by withdrawal is capable of inducing alterations in DH TNF-α expression. The second set of experiments examined whether DH TNF-α expression is functionally relevant to the development of enhanced fear learning. We identified an increase of TNF-α immunoreactivity and positive cells at 0, 24, and 48 h into withdrawal in the dentate gyrus DH subregion. Interestingly, intra-DH infusions of etanercept (TNF-α inhibitor) 0, 24, and 48 h into heroin withdrawal prevented the development of enhanced fear learning and mitigated withdrawal-induced weight loss. Overall, these findings provide insight into the role of TNF-α in opioid withdrawal and the development of anxiety disorders such as PTSD.
n-Nonane hydroisomerization over hierarchical SAPO-11-based catalysts with sodium dodecylbenzene sulfonate as a dispersant
Authors: Wen Cheng-Long , Xu Jun-Dong , Wang Xue-Man , Fan Yu
Published: February 2021

To enhance the gasoline octane number, low-octane linear n-alkanes should be converted into their high-octane di-branched isomers via n-alkane hydroisomerization. Therefore, hierarchical SAPO-11-based catalysts are prepared by adding different contents of sodium dodecylbenzene sulfonate (SDBS), and they are applied in n-nonane hydroisomerization. When n(SDBS)/n(SiO2) is less than or equal to 0.125, the synthesized hierarchical molecular sieves are all pure SAPO-11, and as the SDBS content increases, the submicron particle size decreases, and the external surface area (ESA) increases. Additionally, these hierarchical SAPO-11 have smaller submicron particles and higher ESA values than conventional SAPO-11. When n(SDBS)/n(SiO2) is greater than 0.125, with increasing SDBS content (n(SDBS)/n(SiO2) = 0.25), the synthesized SAPO-11 contains amorphous materials, which leads to a decline in the ESA; with the further increase in SDBS content (n(SDBS)/n(SiO2) = 0.5), the products are all amorphous materials. These results indicate that in the case of n(SDBS)/n(SiO2) = 0.125, the synthesized SAPO-11 molecular sieve (S–S3) has the most external Brønsted acid centers and the highest ESA of these SAPO-11, and these advantages favor generation of the di-branched isomers in hydrocarbon hydroisomerization. Among these Pt/SAPO-11 catalysts, Pt/S–S3 displays the highest selectivity to entire isomers (83.4%), the highest selectivity to di-branched isomers (28.1%) and the minimum hydrocracking selectivity (15.7%) in n-nonane hydroisomerization.
Wettability alteration and retention of mixed polymer-grafted silica nanoparticles onto oil-wet porous medium
Authors: Daneshmand Hamid , Rezaeinasab Masoud , Asgary Masoud , Karimi Meysam
Published: February 2021

Enhanced oil recovery (EOR) processes are applied to recover trapped or residual oil in the reservoir rocks after primary and secondary recovery methods. Changing the wettability of the rock from oil-wet to water-wet is named wettability alteration. It is an important factor for EOR. Due to their unique properties, nanoparticles have gained great attention for improving oil recovery. Despite the promising results, the main challenges of applying nanoparticles are related to the colloidal stability of the nanofluids in the harsh conditions of the reservoirs. In recent years, polymer-grafted nanoparticles have been considered as novel promising materials for EOR. The obtained results showed that adding a hydrophobic agent trimethoxy (propyl) silane on the surface of modified silica nanoparticles with polyethylene glycol methyl ether has an effective role in improving retention and wettability alteration, especially in the oil-wet substrate due to hydrophobic interaction. The modified silica nanoparticle by mixed polyethylene glycol methyl ether (Mn ~ 5000) and trimethoxy (propyl) silane showed a proper performance at a concentration of 1000 ppm and a salinity range of 2000–40,000 ppm. The obtained findings can help for a better understanding of the silica nanofluid modification with both hydrophilic and hydrophobic agents for the EOR application of near-wellbore.
Major Depressive Disorder: Advances in Neuroscience Research and Translational Applications
Authors: Li Zezhi , Ruan Meihua , Chen Jun , Fang Yiru
Published: February 2021

Major depressive disorder (MDD), also referred to as depression, is one of the most common psychiatric disorders with a high economic burden. The etiology of depression is still not clear, but it is generally believed that MDD is a multifactorial disease caused by the interaction of social, psychological, and biological aspects. Therefore, there is no exact pathological theory that can independently explain its pathogenesis, involving genetics, neurobiology, and neuroimaging. At present, there are many treatment measures for patients with depression, including drug therapy, psychotherapy, and neuromodulation technology. In recent years, great progress has been made in the development of new antidepressants, some of which have been applied in the clinic. This article mainly reviews the research progress, pathogenesis, and treatment of MDD.
Homegarden diversity and food security in southern Mexico
Authors: Castañeda-Navarrete Jennifer
Published: February 2021

Home gardens are recognised in the literature for their contribution to food security, yet the process by which agrobiodiversity and household characteristics mediate this relationship is less well understood. This paper contributes to fill this research gap by drawing on a multi-site case study in the Yucatán region in Mexico. By applying regression analysis, the significance of the association between home garden diversity and food security is confirmed. Plant diversity is found to have a positive association with food consumption scores and the frequency of vegetable intakes. The number of animals used for food purposes is also found to have positive and significant associations with food consumption scores and frequency of meat intakes. However, the dimension and the significance of these positive associations were found to vary among communities and quantiles of the distribution of food security measures. In the households studied, younger individuals and better-educated people, males and Spanish speakers were more likely to engage in jobs in urban areas. Engagement in urban jobs was found to involve complementarities with the overall plant diversity of home gardens, but also trade-offs with the diversity of vegetables and other herbs used for food purposes and with the abundance of animals raised for food purposes.
Parental Involvement Policies for Minors Seeking Abortion in the Southeast and Quality of Care
Authors: White Kari , Narasimhan Subasri , A. Hartwig Sophie , Carroll Erin , McBrayer Alexandra , Hubbard Samantha , Rebouché Rachel , Kottke Melissa , Hall Kelli Stidham
Published: February 2021

Introduction

Thirty-seven states require minors seeking abortion to involve a parent, either through notification or consent. Little research has examined how implementation of these laws affect service delivery and quality of care for those who involve a parent.

Methods

Between May 2018 and September 2019, in-depth interviews were conducted with 34 staff members involved in scheduling, counseling, and administration at abortion facilities in three Southeastern states. Interviews explored procedures for documenting parental involvement, minors’ and parents’ reactions to requirements, and challenges with implementation and compliance. Both inductive and deductive codes, informed by the Institute of Medicine’s healthcare quality framework, were used in the thematic analysis.

Results

Parental involvement laws adversely affected four quality care domains: efficiency, patient-centeredness, timeliness, and equity. Administrative inefficiencies stemmed from the extensive documentation needed to prove an adult’s relationship to a minor, increasing the time and effort needed to comply with state reporting requirements. If parents were not supportive of their minor’s decision, participants felt they had a duty to intervene to ensure the minor’s decision and needs remained centered. Staff further noted that delays to timely care accumulated as minors navigated parental involvement and other state mandates, pushing some beyond gestational age limits. Lower income families and those with complex familial arrangements had greater difficulty meeting state requirements.

Conclusions

Parental involvement mandates undermine health service delivery and quality for minors seeking abortion services in the Southeast.

Policy Implications

Removing parental involvement requirements would protect minors’ reproductive autonomy and support the provision of equitable, patient-centered healthcare.