bluuebunny/crossref_metadata_2025 huggingface dataset:
A machine learning-based strategy to elucidate the identification of antibiotic resistance in bacteria
Authors: Parthasarathi K. T. Shreya , Gaikwad Kiran Bharat , Rajesh Shruthy , Rana Shweta , Pandey Akhilesh , Singh Harpreet , Sharma Jyoti
Published: June 2024

Microorganisms, crucial for environmental equilibrium, could be destructive, resulting in detrimental pathophysiology to the human host. Moreover, with the emergence of antibiotic resistance (ABR), the microbial communities pose the century’s largest public health challenges in terms of effective treatment strategies. Furthermore, given the large diversity and number of known bacterial strains, describing treatment choices for infected patients using experimental methodologies is time-consuming. An alternative technique, gaining popularity as sequencing prices fall and technology advances, is to use bacterial genotype rather than phenotype to determine ABR. Complementing machine learning into clinical practice provides a data-driven platform for categorization and interpretation of bacterial datasets. In the present study, k-mers were generated from nucleotide sequences of pathogenic bacteria resistant to antibiotics. Subsequently, they were clustered into groups of bacteria sharing similar genomic features using the Affinity propagation algorithm with a Silhouette coefficient of 0.82. Thereafter, a prediction model based on Random Forest algorithm was developed to explore the prediction capability of the k-mers. It yielded an overall specificity of 0.99 and a sensitivity of 0.98. Additionally, the genes and ABR drivers related to the k-mers were identified to explore their biological relevance. Furthermore, a multilayer perceptron model with a hamming loss of 0.05 was built to classify the bacterial strains into resistant and non-resistant strains against various antibiotics. Segregating pathogenic bacteria based on genomic similarities could be a valuable approach for assessing the severity of diseases caused by new bacterial strains. Utilization of this strategy could aid in enhancing our understanding of ABR patterns, paving the way for more informed and effective treatment options.
We Are Not on Track: Greenhouse Gas Emissions Are Higher Than Ever!
Authors: Peters Glen P. , Meinshausen Malte
Published: June 2024

Even though we have known about climate change for more than 30 years, humans are releasing higher amounts of greenhouse gases, like carbon dioxide, than ever before. Scientists have shown that stopping carbon dioxide emissions could prevent global warming from getting worse. The single biggest source of greenhouse gas emissions is burning fossil fuels for electricity or transportation. Technologies like solar and wind power, electric vehicles, and advanced batteries can replace fossil fuels and help reduce emissions while also decreasing other problems like air pollution. Many countries have promised to stop their carbon dioxide emissions, and if their promises are backed up with laws, then global warming could be limited. Unfortunately, current laws are not good enough. We need to ensure that countries meet their promises, so that society can avoid the worst effects of global warming.
Biopotential of rhizobacteria to improve growth and phytochemical content in Javanese ginseng (Talinum paniculatum) herbal plant
Authors: Suriani Ni Luh , Suprapta Dewa Ngurah , Suarsana I. Nyoman , Resiani Ni Made Delly , Perveen Kahkashan , Bukhari Najat A. , Ei Enshasy Hesham Ali , Ho Ting Seng , Sayyed R. Z.
Published: June 2024

Introduction

Developing organic herbal crops provides chemical-free herbs to support environmentally friendly and sustainable farming. One way in agricultural biotechnology to grow herbal organic crops is to use rhizobacteria. The herbal plant used in this study is the Javanese ginseng plant (Talinum paniculatum), which contains phytochemicals that increase stamina.

Methods

The study used four rhizobacteria to enhance the growth and phytochemistry of T. paniculatum leaves; the target phytochemical compounds analyzed in Javanese ginseng leaves were total flavonoids, total phenols, and antioxidants (IC50%). The four selected rhizobacteria can produce indole-3-acetic acid (IAA), fix nitrogen, and dissolve phosphate. Thus, high-quality T. paniculatum leaves were obtained as herbal tea ingredients. The pattern used is a random group pattern in the greenhouse.

Results

The results of the study showed that the use of rhizobacteria at 2% concentrations had a real effect on plant growth parameters such as plant height, leaf area, root length, wet weight and dry weight of the plant, and phytochemical content like total flavonoids, total phenols, IC50%, total chlorophyll, content of T. paniculatum leave plant when compared to the control. The four rhizobacteria used can produce the IAA, fix nitrogen, and dissolve phosphorus.

Discussion

The four rhizobacteria can also colonize the root of the T. paniculatum plant. The four Rhizobzcteria used significantly affected the growth and phytochemical content of T. paniculatum leaves as an ingredient in herbal tea at a concentration of 2% compared to controls. The four rhizobacteria can produce IAA hormones, fix nitrogen, and dissolve phosphorus. All four rhizobacteria can colonize the roots of T. paniculatum plants. These four rhizobacteria can be used as alternative methods in developing organic farming systems and can also be used practically in the field by farmers. It is necessary to research the application of rhizobacteria to other crops to support sustainable agriculture.
Developmental ability of Hanwoo muscle satellite cells under culture conditions mimicking the in vivo environment
Authors: Park Gyutae , Park Sanghun , Oh Sehyuk , Lee Sol-Hee , Choi Jungseok
Published: June 2024

Cultivated meat refers to edible meat obtained by proliferating cells without killing livestock in a laboratory. The selection of donor animals is a crucial factor for efficient cell culture production. Hanwoo is a native Korean taurine cattle breed raised as livestock in Korea since before 2000 B.C. Cells isolated from Hanwoo, which has little genetic diversity, are expected to be advantageous in cell culture because of the existence of fewer individual differences. However, cells collected from Hanwoo are in a state where efficient culture conditions have not been established. Therefore, in this study, we investigated the effects of mimicking an in vivo environment on the proliferation and differentiation of Hanwoo muscle satellite cells. The culture conditions consisted of CON (37°C/20% O2), T1 (37°C/2% O2), T2 (39°C/20% O2), and T3 (39°C/2% O2). Cell numbers decreased and expression levels of PAX7 and MYF5 decreased at a temperature of 39°C (p < 0.05). Conversely, 2% oxygen increased the number of cells and expression levels of PAX7 and MYF5 (p < 0.05). A temperature of 39°C inhibited the proliferation of Hanwoo muscle satellite cells by reducing the expression of PAX7 and MYF5 (p < 0.05). Conversely, 2% oxygen promoted the proliferation of Hanwoo muscle satellite cells by enhancing the expression of PAX7 and MYF5 (p < 0.05). During differentiation, a temperature of 39°C improved the myotube area and fusion index (p < 0.05). The RT-qPCR and Western blotting results revealed that a culture temperature of 39°C increased expression levels of the MYH2 gene and DES and MYOG proteins (p < 0.05). Additionally, an interactive condition increased expression levels of MYOD1, DES, and MYOG genes (p < 0.05). These results indicated that a temperature of 39°C promoted the differentiation of Hanwoo muscle satellite cells by increasing DES and MYOG protein expression. Thus, the production of cultivated meat using Hanwoo muscle satellite cells is expected to be efficient under 2% oxygen for proliferation and 39°C for differentiation.
Patient and Provider Attitudes and Preferences Regarding Early Palliative Care Delivery for Patients with Advanced Gastrointestinal Cancers: A Prospective Survey
Authors: Levine Oren , Bainbridge Daryl , Pond Gregory R. , Slaven Marissa , Dhesy-Thind Sukhbinder , Sussman Jonathan , Meyer Ralph M.
Published: June 2024

Early integrated palliative care (EIPC) for patients with advanced cancers requires the involvement of family doctors (FDs) and oncologists. We compared attitudes between patients and their providers regarding the delivery of EIPC. Patients with newly diagnosed incurable gastrointestinal (GI) cancer at a tertiary cancer centre in Ontario, Canada, were surveyed using a study-specific instrument regarding the importance of and preferences for accessing support across eight domains of palliative care. Physicians within the circle of care completed a parallel survey for each patient. The concordance between patient and physician responses was analyzed. A total of 66 patients were surveyed (median age 69, 35% female). All had an oncologist, 12% had a specialist palliative care provider (SPC), and 97% had an FD, but only 41% listed the FD as part of the care team. In total, 95 providers responded (oncologist = 68, FD = 21, SPC = 6; response rate 92%; 1–3 physician responses per patient). Disease management and physical concerns were most important to patients. Patients preferred to access care in these domains from oncologists or SPCs. For all other domains, most patients attributed primary responsibility to self or family rather than any healthcare provider. Thus, concordance was poor between patient and physician responses. Across most domains of palliative care, we found low agreement between cancer patients and their physicians regarding responsibilities for care, with FDs appearing to have limited involvement at this stage.
Abstracts of the 39th Annual CAPO Conference—Building Hope: Integrating Sustainable, Innovative and Accessible Care in Psychosocial Oncology 6 to 7 June 2024
Authors: Traversa Peter , Howell Doris
Published: June 2024

On behalf of the Canadian Association of Psychosocial Oncology, we are pleased to present the Abstracts from the 2024 Annual Conference, titled “Building Hope: Integrating Sustainable, Innovative and Accessible Care in Psychosocial Oncology”. The Conference was held in Calgary from 6 June 2024 to 7 June 2024. This conference brought together key stakeholders including multidisciplinary professionals from nursing, psychology, psychiatry, social work, spiritual care, nutrition, medicine, rehabilitation medicine, occupational health and radiation therapy for both adult and pediatric populations. Participants included clinicians, researchers, educators in cancer care, community-based organizations and patient representatives. Patients, caregivers and family members presented abstracts that speak to their role in managing cancer experiences and care. Over one hundred (150) abstracts were selected for presentation as symposia, 20 min oral presentations, 10 min oral presentations, 90 min workshops and poster presentations. We congratulate all the presenters on their research work and contribution.
Outcome of an Accelerated Treatment Algorithm for Patients Developing Diarrhea as a Complication of Ipilimumab-Based Cancer Immunotherapy in a Community Practice
Authors: Ho Clarice , Samlowski Wolfram
Published: June 2024

Immune-mediated diarrhea represents a serious complication of checkpoint inhibitor therapy, especially following ipilimumab-based treatment. Efficient diagnosis and control of diarrhea remains an ongoing challenge. We developed an accelerated management paradigm for patients with ipilimumab-induced diarrhea. Patients who developed significant diarrhea (>five loose stools/day) were presumed to be developing immune colitis. Therapy was interrupted and patients were treated with a methylprednisolone dose pack. If diarrhea was not completely resolved, high-dose steroids and infliximab were promptly added. Only non-responding patients underwent further evaluation for infection or other causes of diarrhea. A total of 242 patients were treated with ipilimumab-based regimens. Forty-six developed significant diarrhea (19%) and thirty-four (74.4%) had a rapid resolution of diarrhea following glucocorticosteroid and infliximab treatment. The median time to resolution of diarrhea was only 8.5 ± 16.4 days. Accelerated treatment for presumed immune-mediated diarrhea resulted in the rapid control of symptoms in the majority of patients. There were no intestinal complications or deaths. Immunosuppressive therapy for diarrhea did not appear to decrease the remission rate or survival. After the control of diarrhea, most patients were able to continue their planned immunotherapy. Further testing in 11/46 patients with unresponsive diarrhea revealed additional diagnoses, allowing their treatment to be adjusted.
Correction: Sandlersky et al. Multispectral Remote Sensing Data Application in Modelling Non-Extensive Tsallis Thermodynamics for Mountain Forests in Northern Mongolia. Entropy 2023, 25, 1653
Authors: Sandlersky Robert , Petrzhik Nataliya , Jargalsaikhan Tushigma , Shironiya Ivan
Published: June 2024

There were some errors in the original publication [...]
Smart System for Reducing Standby Energy Consumption in Residential Appliances
Authors: Gheorghe Andrei Cosmin , Andrei Horia , Diaconu Emil , Andrei Paul Cristian
Published: June 2024

Residential consumption represents one of the most important percentages of total electricity consumption. A considerable number of household appliances consume energy even when they are not in operation, i.e., they are in the so-called standby state, thus producing additional costs, which become significant over time. In this context, one method to solve this problem is to develop a smart system capable of severing the power connection to devices in standby mode, thereby conserving energy and reducing the energy costs. The first step in the design of this system consists of the identification and accurate measurement of the standby state, which was carried out for three of the most common household appliances. Then, by using an ESP32 microcontroller, a system was designed to manage the operation of a relay module based on the current consumption of the connected equipment. Control over the system was achieved through a web application that works across all devices equipped with a web browser, offering functionalities to adjust current value time delays and to manually switch the system on or off. Finally, the deployment of this system across the three appliances studied led to a reduction in the energy consumption in standby mode of 26.68 kWh per month.
Graphene Far-Infrared Irradiation Can Effectively Relieve the Blood Pressure Level of Rat Untr-HT in Primary Hypertension
Authors: Lu Guanjie , Guo Haotong , Zhang Yi , Zhang Meng , Zhang Tao , Hu Ge , Zhang Qian
Published: June 2024

Graphene, when electrified, generates far-infrared radiation within the wavelength range of 4 μm to 14 μm. This range closely aligns with the far-infrared band (3 μm to 15 μm), which produces unique physiological effects. Contraction and relaxation of vascular smooth muscle play a significant role in primary hypertension, involving the nitric oxide-soluble guanylate cyclase–cyclic guanosine monophosphate pathway and the renin–angiotensin–aldosterone system. This study utilized spontaneously hypertensive rats (SHRs) as an untr-HT to investigate the impact of far-infrared radiation at specific wavelengths generated by electrified graphene on vascular smooth muscle and blood pressure. After 7 weeks, the blood pressure of the untr-HT group rats decreased significantly with a notable reduction in the number of vascular wall cells and the thickness of the vascular wall, as well as a decreased ratio of vessel wall thickness to lumen diameter. Additionally, blood flow perfusion significantly increased, and the expression of F-actin in vascular smooth muscle myosin decreased significantly. Serum levels of angiotensin II (Ang-II) and endothelin 1 (ET-1) were significantly reduced, while nitric oxide synthase (eNOS) expression increased significantly. At the protein level, eNOS expression decreased significantly, while α-SMA expression increased significantly in aortic tissue. At the gene level, expressions of eNOS and α-SMA in aortic tissue significantly increased. Furthermore, the content of nitric oxide (NO) in the SHR’s aortic tissue increased significantly. These findings confirm that graphene far-infrared radiation enhances microcirculation, regulates cytokines affecting vascular smooth muscle contraction, and modifies vascular morphology and smooth muscle phenotype, offering relief for primary hypertension.
Analysis of CYP1B1 Polymorphisms in Lung Cancer Patients Using Novel, Quick and Easy Methods Based on CAPS and ACRS-PCR Techniques
Authors: Dąbrowski Adam , Nowicki Maciej , Budzyńska Aleksandra , Suchodolski Jakub , Ogórek Rafał , Chabowski Mariusz , Przywara Katarzyna
Published: June 2024

Within the sequence of the CYP1B1 gene, more than 50 polymorphisms, resulting from single-nucleotide polymorphisms (SNPs), have been described. Some of them play an important role as specific genetic markers in the process of carcinogenesis and for therapeutic purposes. In this publication, we present methods we have developed that enable the specific and unambiguous identification of four polymorphisms that result in amino acid changes: c. 142C > G, c. 355G > T, c. 1294C > G, and c. 1358A > G. Our studies are based on cleaved amplified polymorphic sequences (CAPSs) and artificially created restriction site (ACRS) PCR techniques; therefore, they require only basic laboratory equipment and low financial outlays. Utilizing the described methods allows for the reduction of research time and cost, and the minimization of errors. Their effectiveness and efficiency depend on the careful design of appropriate primers and the precise selection of suitable restriction enzymes. As a result, further confirmation by sequencing is not necessary. Using the developed method, we examined 63 patients diagnosed with lung cancer and observed a 1.5 to 2.1 times higher frequency of the analyzed single-nucleotide polymorphisms compared to the frequency in the European population.
Sustainable Synthesis of the Active Pharmaceutical Ingredient Atenolol in Deep Eutectic Solvents
Authors: Procopio Debora , Siciliano Carlo , Perri Assunta , Guillena Gabriela , Ramón Diego J. , Di Gioia Maria Luisa
Published: June 2024

Atenolol, one of the top five best-selling drugs in the world today used to treat angina and hypertension, and to reduce the risk of death after a heart attack, faces challenges in current synthetic methods to address inefficiencies and environmental concerns. The traditional synthesis of this drug involves a process that generates a large amount of waste and other by-products that need disposal. This study presents a one-pot DES-based sustainable protocol for synthesizing atenolol. The use of the DES allowed the entire process to be conducted with no need for additional bases or catalysts, in short reaction times, under mild conditions, and avoiding chromatographic purification. The overall yield of atenolol was 95%. The scalability of the process to gram-scale production was successfully demonstrated, emphasizing its potential in industrial applications. Finally, the ‘greenness’ evaluation, performed using the First Pass CHEM21 Metrics Toolkit, highlighted the superiority in terms of the atom economy, the reaction mass efficiency, and the overall process mass intensity of the DES-based synthesis compared with the already existing methods.
Genetic Diversity and Population Structure of Rhodeus uyekii in the Republic of Korea Revealed by Microsatellite Markers from Whole Genome Assembly
Authors: Kim Kang-Rae , Park So Young , Jeong Ju Hui , Hwang Yujin , Kim Heesoo , Sung Mu-Sung , Yu Jeong-Nam
Published: June 2024

This study is the first report to characterize the Rhodus uyekii genome and study the development of microsatellite markers and their markers applied to the genetic structure of the wild population. Genome assembly was based on PacBio HiFi and Illumina HiSeq paired-end sequencing, resulting in a draft genome assembly of R. uyekii. The draft genome was assembled into 2652 contigs. The integrity assessment of the assemblies indicates that the quality of the draft assemblies is high, with 3259 complete BUSCOs (97.2%) in the database of Verbrata. A total of 31,166 predicted protein-coding genes were annotated in the protein database. The phylogenetic tree showed that R. uyekii is a close but distinct relative of Onychostoma macrolepis. Among the 10 fish genomes, there were significant gene family expansions (8–2387) and contractions (16–2886). The average number of alleles amplified by the 21 polymorphic markers ranged from 6 to 23, and the average PIC value was 0.753, which will be useful for evolutionary and genetic analysis. Using population genetic analysis, we analyzed genetic diversity and the genetic structures of 120 individuals from 6 populations. The average number of alleles per population ranged from 7.6 to 9.9, observed heterozygosity ranged from 0.496 to 0.642, and expected heterozygosity ranged from 0.587 to 0.783. Discriminant analysis of principal components According to the analysis method, the population was divided into three populations (BS vs. DC vs. GG, GC, MS, DC). In conclusion, our study provides a useful resource for comparative genomics, phylogeny, and future population studies of R. uyekii.
Generation of Myeloid-Derived Suppressor Cells Mediated by MicroRNA-125a-5p in Melanoma
Authors: Lasser Samantha , Ozbay Kurt Feyza Gul , Fritz Lennart , Gutzeit Nina , De La Torre Carolina , Altevogt Peter , Utikal Jochen , Umansky Viktor
Published: June 2024

The ability of tumor-derived extracellular vesicles (EVs) to modulate the function of myeloid cells is widely recognized. Hence, a comprehensive understanding of the distinct components associated with EVs and the signals that they deliver to myeloid cells could provide potential approaches to impede the immunosuppression by myeloid-derived suppressor cells (MDSCs). We investigated melanoma EV-associated microRNAs (miRs) using the RET transgenic melanoma mouse model and simulated their transfer to normal myeloid cells by transfecting immature mouse myeloid cells and human monocytes. We observed elevated levels of miR-125a-5p, -125b-5p, and let-7e-5p in mouse melanoma-infiltrating MDSCs. In addition, miR-125a-5p levels in the tumor microenvironment correlated with mouse melanoma progression. The delivery of miR-125a-5p, alone or in combination with let-7e-5p and miR-99b-5p from the same genomic cluster, to normal myeloid cells resulted in their conversion to MDSC-like cells. Our findings indicate that miR-125a-5p could modulate myeloid cell activation in the melanoma microenvironment via a NF-κB-dependent mechanism.
Exploring Thyroid Function after Kidney Transplantation: The Complex Interplay Unacknowledged in Post-Transplant Care
Authors: Jelić Pranjić Ita , Orlić Lidija , Carević Ana , Vrdoljak Margeta Tea , Šimić Jelena , Bubić Ivan
Published: June 2024

Background/Objectives: The interplay between thyroid function and kidney graft function following kidney transplantation remains incompletely understood. Thyroid disorders are more prevalent in kidney transplant recipients than in the general population and are associated with poorer outcomes. Methods: This prospective, single-center study was designed to estimate thyroid function (thyroid-stimulating hormone (TSH), triiodothyronine (T3), free triiodothyronine (FT3), thyroxine (T4), free thyroxine (FT4), as well as anti-thyroid peroxidase antibody (anti-TPO), anti-thyroglobulin antibody (anti-Tg), and thyroid-stimulating immunoglobulin (TSI)) and its influence on kidney graft function among a cohort of 23 kidney transplant recipients during a follow-up period of 12 months. Results: Significantly increased levels of T4 and T3 were observed 12 months post-transplantation, with FT3 levels increasing significantly after 6 months. The prevalence of immeasurably low anti-Tg antibodies rose during follow-up. Initially, 8% of patients showed positive TSI, which turned negative for all after 6 months. A statistically significant correlation was found between the initial TSH and the estimated glomerular filtration rate (eGFR) value 6 months after transplantation (p = 0.023). The graft function was stable. Proteinuria was statistically significantly lower 12 months after transplantation. Conclusions: Identifying additional risk factors, understanding their impact on kidney graft function, and recognizing cardiovascular comorbidities could enhance patient care. Notably, this study marks the first prospective investigation into thyroid function after kidney transplantation in Croatia, contributing valuable insights to the global understanding of this complex interplay.
Structural, Biophysical, and Computational Studies of a Murine Light Chain Dimer
Authors: Arriaza Ricardo H. , Kapingidza A. Brenda , Dolamore Coleman , Khatri Kriti , O’Malley Andrea , Glesner Jill , Wuenschmann Sabina , Hyduke Noah P. , Easley William , Chhiv Charline , Pomés Anna , Chruszcz Maksymilian
Published: June 2024

Antibodies are widely used in medicinal and scientific research due to their ability to bind to a specific antigen. Most often, antibodies are composed of heavy and light chain domains. Under physiological conditions, light chains are produced in excess, as compared to the heavy chain. It is now known that light chains are not silent partners of the heavy chain and can modulate the immune response independently. In this work, the first crystal structure of a light chain dimer originating from mice is described. It represents the light chain dimer of 6A8, a monoclonal antibody specific to the allergen Der f 1. Building on the unexpected occurrence of this kind of dimer, we have demonstrated that this light chain is stable in solution alone. Moreover, enzyme-linked immunosorbent assays (ELISA) have revealed that, when the light chain is not partnered to its corresponding heavy chain, it interacts non-specifically with a wide range of proteins. Computational studies were used to provide insight on the role of the 6A8 heavy chain domain in the specific binding to Der f 1. Overall, this work demonstrates and supports the ongoing notion that light chains can function by themselves and are not silent partners of heavy chains.
Real-Time Trajectory Smoothing and Obstacle Avoidance: A Method Based on Virtual Force Guidance
Authors: Su Yongbin , Lin Chenying , Liu Tundong
Published: June 2024

In dynamic environments, real-time trajectory planners are required to generate smooth trajectories. However, trajectory planners based on real-time sampling often produce jerky trajectories that necessitate post-processing steps for smoothing. Existing local smoothing methods may result in trajectories that collide with obstacles due to the lack of a direct connection between the smoothing process and trajectory optimization. To address this limitation, this paper proposes a novel trajectory-smoothing method that considers obstacle constraints in real time. By introducing virtual attractive forces from original trajectory points and virtual repulsive forces from obstacles, the resultant force guides the generation of smooth trajectories. This approach enables parallel execution with the trajectory-planning process and requires low computational overhead. Experimental validation in different scenarios demonstrates that the proposed method not only achieves real-time trajectory smoothing but also effectively avoids obstacles.
Classification of Visually Induced Motion Sickness Based on Phase-Locked Value Functional Connectivity Matrix and CNN-LSTM
Authors: Shen Zhenqian , Liu Xingru , Li Wenqiang , Li Xueyan , Wang Qiang
Published: June 2024

To effectively detect motion sickness induced by virtual reality environments, we developed a classification model specifically designed for visually induced motion sickness, employing a phase-locked value (PLV) functional connectivity matrix and a CNN-LSTM architecture. This model addresses the shortcomings of traditional machine learning algorithms, particularly their limited capability in handling nonlinear data. We constructed PLV-based functional connectivity matrices and network topology maps across six different frequency bands using EEG data from 25 participants. Our analysis indicated that visually induced motion sickness significantly alters the synchronization patterns in the EEG, especially affecting the frontal and temporal lobes. The functional connectivity matrix served as the input for our CNN-LSTM model, which was used to classify states of visually induced motion sickness. The model demonstrated superior performance over other methods, achieving the highest classification accuracy in the gamma frequency band. Specifically, it reached a maximum average accuracy of 99.56% in binary classification and 86.94% in ternary classification. These results underscore the model’s enhanced classification effectiveness and stability, making it a valuable tool for aiding in the diagnosis of motion sickness.
Development of a Sensitive Colorimetric Indicator for Detecting Beef Spoilage in Smart Packaging
Authors: Karimi Alavijeh Dariush , Heli Bentolhoda , Ajji Abdellah
Published: June 2024

This study aimed to fabricate and characterize a novel colorimetric indicator designed to detect ammonia (NH3) and monitor meat freshness. The sensing platform was constructed using electrospun nanofibers made from polylactic acid (PLA), which were then impregnated with anthocyanins as a natural pH-sensitive dye, extracted from red cabbage. This research involved investigating the relationship between the various concentrations of anthocyanins and the colorimetric platform’s efficiency when exposed to ammonia vapor. Scanning electron microscope (SEM) results were used to examine the morphology and structure of the nanofiber mats before and after the dip-coating process. The study also delved into the selectivity of the indicator when exposed to various volatile organic compounds (VOCs) and their stability under extreme humidity levels. Furthermore, the platform’s sensitivity was evaluated as it encountered ammonia (NH3) in concentrations ranging from 1 to 100 ppm, with varying dye concentrations. The developed indicator demonstrated an exceptional detection limit of 1 ppm of MH3 within just 30 min, making it highly sensitive to subtle changes in gas concentration. The indicator proved effective in assessing meat freshness by detecting spoilage levels in beef over time. It reliably identified spoilage after 10 h and 7 days, corresponding to bacterial growth thresholds (107 CFU/mL), both at room temperature and in refrigerated environments, respectively. With its simple visual detection mechanism, the platform offered a straightforward and user-friendly solution for consumers and industry professionals alike to monitor packaged beef freshness, enhancing food safety and quality assurance.
High-Performance Refractive Index and Temperature Sensing Based on Toroidal Dipole in All-Dielectric Metasurface
Authors: Zhao Jingjing , Fan Xinye , Fang Wenjing , Xiao Wenxing , Sun Fangxin , Li Chuanchuan , Wei Xin , Tao Jifang , Wang Yanling , Kumar Santosh
Published: June 2024

This article shows an all-dielectric metasurface consisting of “H”-shaped silicon disks with tilted splitting gaps, which can detect the temperature and refractive index (RI). By introducing asymmetry parameters that excite the quasi-BIC, there are three distinct Fano resonances with nearly 100% modulation depth, and the maximal quality factor (Q-factor) is over 104. The predominant roles of different electromagnetic excitations in three distinct modes are demonstrated through near-field analysis and multipole decomposition. A numerical analysis of resonance response based on different refractive indices reveals a RI sensitivity of 262 nm/RIU and figure of merit (FOM) of 2183 RIU−1. This sensor can detect temperature fluctuations with a temperature sensitivity of 59.5 pm/k. The proposed metasurface provides a novel method to induce powerful TD resonances and offers possibilities for the design of high-performance sensors.
Electro-tranquilization in the relief of pre-nosological forms of psychosomatic disorders
Authors:   FSBEI HE I. M. Sechenov First Moscow State Medical University, Moscow, Darenskiy I. D.
Published: June 2024

Tranquilizers, widely used in the treatment of distress, neuroses, and psychosomatic disorders, have excessive sedative effect and narcogenicity. Transcranial pulsed current stimulation (tPCS) (electrotranquilization — ET, transcranial electrical stimulation) as non-pharmacological impact with a tranquilizing effect is devoid of these disadvantages. The purpose of the study was to determine the effectiveness of using ET in relation to distress, somatization, depression, and anxiety as the initial manifestations of psychosomatic disorders. Methods. Eighty patients who consulted a psychotherapist for somato-vegetative disorders were randomly divided into a group that received weekly ET treatment and a group that received placebo treatment. The severity of disorders and their dynamics were assessed using the Four-Dimensional Symptom Questionnaire (4DSQ). Results. After a week of ET treatment, rates of anxiety, depression, somatization, and distress decreased. Conclusions. The use of ET in patients with psychosomatic disorders at a pre-nosology stage leads to a significant reduction in the severit y of these disorders.
Clinical features of auto-aggressive behavior in borderline personality disorder: a review of current research
Authors:   Federal State Budgetary Educational Institution of Higher Education «Ryazan State Medical University named after Academician I. P. Pavlov» of the Ministry of Health of the Russian Federation, Trabelsi F. , Shustov D. I. ,   Federal State Budgetary Educational Institution of Higher Education «Ryazan State Medical University named after Academician I. P. Pavlov» of the Ministry of Health of the Russian Federation, Merinov A. V. ,   Federal State Budgetary Educational Institution of Higher Education «Ryazan State Medical University named after Academician I. P. Pavlov» of the Ministry of Health of the Russian Federation, Petrov D. S. ,   Federal State Budgetary Educational Institution of Higher Education «Ryazan State Medical University named after Academician I. P. Pavlov» of the Ministry of Health of the Russian Federation, Fedotov I. A. ,   Federal State Budgetary Educational Institution of Higher Education «Ryazan State Medical University named after Academician I. P. Pavlov» of the Ministry of Health of the Russian Federation
Published: June 2024

Borderline personality disorder (BPD) is a common psychiatric disorder characterized by severe auto-aggression (AA) in the form of suicide attempts (SA) and non-suicidal self-injury (NSSI). The purpose of this review was to describe the current knowledge about the clinical features of self-harming behavior in borderline personality disorder. Materials and methods: The eLibrary, PubMed, CNKI, and Google Scholar databases were searched for articles on the relevant search query for the last 11 years. As a result, 28 articles were selected and summarized (a total of 7,284 BPD patients and 134 controls were included). Results: Suicidal attempts occur in 75 % of BPD patients, and NSSIs — in 90 %. AA behavior varies with age: adolescents tend to NSSIs, while adults more often undertake SAs. Depending on the type of clinical dynamics, AA in BPD may be impulsive, demonstrative, associated with comorbid addiction, or occur in the context of depersonalization. Among BPD symptoms, feelings of chronic emptiness, avoidance of abandonment, emotional instability, and identity disturbance are most commonly associated with auto-aggression. Risk factors for self-harming behavior in BPD include traumatic childhood events, especially physical and sexual abuse, and stress caused by microsocial conflict. Men with BPD and AA more often have comorbid narcissistic, antisocial, paranoid, and schizotypal personality disorders, as well as substance use disorders. Women with borderline personality disorder and AA tend to suffer more from addictive and obsessive-compulsive personality disorders, as well as eating disorders, particularly bulimia nervosa. In conclusion, self-harming behavior in BPD has many clinical features and can manifest in different forms. It is always necessary to study the predominant clinical manifestations, risk factors, and comorbid disorders to be able to assess the severity of AA.
Depression in hemodialysis patients: gender and age analysis
Authors:   Federal State Budgetary Educational Institution of Higher Education “Rostov State Medical University” of the Ministry of Health of the Russian Federation, Rostov-on-Don, Russia, Sannikov A.N. , Rodkin S.V. ,   Federal State Budgetary Educational Institution of Higher Education “Don State Technical University”, Rostovon- Don, Russia, Zaika V.G. ,   Federal State Budgetary Educational Institution of Higher Education “Rostov State Medical University” of the Ministry of Health of the Russian Federation, Rostov-on-Don, Russia
Published: June 2024

The purpose of this work was to estimate the prevalence of depressive disorders in hemodialysis patients and conduct a gender comparison of depression. The study group consisted of 25 men and 25 women with chronic kidney disease receiving hemodialysis therapy. The Hamilton Depression Inventory was used for diagnosis. The prevalence of depression in the study population was 26%. The incidence of depression in women was 12% higher than in men. With increasing age, the level of depression in men went up. Suicidal behavior was observed 4% more often in the group of men than in women.
ARTISTIC CREATIVITY AND DEVELOPMENT OF AESTHETIC THOUGHT: PHILOSOPHICAL REFLECTION
Authors: Novikov Boris , Rudenko Tamara , Potishchuk Olha
Published: June 2024

The research objective. The purpose of the study is to determine the nature, features, functioning mechanisms of artistic creativity, to consider the main stages of the development of aesthetic views. To solve the task, it is necessary to consider the philosophical and aesthetic thought of the past: philosophical searches of thinkers of antiquity, the Middle Ages, the Renaissance, and the New Age, the aesthetic views of German classical idealism, non-classical philosophy and the role of irrational motives in the artistic creativity of the 19th and 20th centuries, modern aesthetic views. A detailed examination of artistic creativity will also contribute to the understanding of the specifics of the artist's creativity in each specific art form, the aesthetic and educational role of artistic works.
The statement of basic materials. Aesthetic thought, like world science, has deep roots, traditions of aesthetic analysis have been formed since ancient times. There are various definitions of artistic creativity, but the properties of works of art and their impact on people are considered through the concept of the beautiful, which is central to aesthetic science. Artistic creativity is able to evoke strong feelings, promotes the development of imagination, fantasy, stimulates and directs the creative activity itself. In aesthetic thought, a huge amount of material was collected and various views were expressed regarding the nature and peculiarities of the functioning of artistic creativity. Artistic creativity has always been the center of attention of researchers, and in the future, it will attract attention, contribute to new creative discoveries, and will be admired.
PHYSICAL THEORY: KEY TERMS AND ITS MATHEMATICAL COMPONENT
Authors: Chumak Mykola , Yefymenko Vasyl`
Published: June 2024

Actual scientific researches and issues analysis. The movement from classical physics to modern physics occurred as a result of the emergence of a number of paradoxes in classical theory. This feature is characteristic to some extent of the electromagnetic theory of Maxwell, Einstein's theory of relativity, etc. The research objective
. We will try to consider the key terms of physical theory and show the role of the mathematical component in its creation. The statement of basic materials. The article deals with some modern problems of the methodology and foundations of physics, in particular: whether mathematical formalisms contain interpretations of themselves or whether they need to be supplemented by interpretive predictions, and if so, how these predictions are formulated; what physical theories describe: physical systems or laboratory operations, either one or another, or neither of them; how the basic concepts of theory should be introduced, by reference to measuring operations, or by means of clear definitions, or axiomatically; how physical theories relate to each other; how theory relates to experiment: directly or with the help of additional theories.
METHODOLOGICAL SYSTEM OF USING CLOUD-ORIENTED OPEN SCIENCE SYSTEMS IN THE PROCESS OF EDUCATION AND PROFESSIONAL DEVELOPMENT OF TEACHERS
Authors: Luparenko Liliia , Marienko Maiia , Shyshkina Mariya
Published: June 2024

Actual scientific researches and issues analysis. The results of the study by E. Yenen and M. Yöntem indicate that the most necessary areas of professional development of teachers are special education, methods of scientific research, participation in projects and relevant professional activities. 14 Dutch institutions of general secondary education have developed and implemented a number of measures related to the concept “School as a professional learning community”. The research of J. Imants and M. M. Van der Wal was to create a model with the potential of professional development and school reform from the position of the teacher at many levels. The research objective. To design a methodical system of using cloud-oriented systems of open science in the process of teaching and professional development of teachers. The statement of basic materials. The creation of a methodical system of training and professional development of teachers involves a number of separate methods of using cloud-oriented components of educational purposes for the formation of teachers' skills in the formation of cloud-oriented systems of open science in the process of their further professional activities. Special attention should be paid to the means of forming and developing a cloud-oriented environment for improving the qualifications of teachers, which may include various services of open science and their combination, separated into appropriate groups.
THE RESULTS OF THE ALL-UKRAINIAN COMPETITION OF ENVIRONMENTAL PROJECTS “ECOPOHLIAD”
Authors: Babiichuk Svitlana
Published: June 2024

Actual scientific researches and issues analysis. In our work, we based on some of the most cited foreign works related to the organization of national and international competitions, in particular, the works of Franca Sangiorgio, Vojsava Gjoni, Wojsaw Joni Nicola, Nicola Fiore, Donato Tarantino, Alberto Basset, Laura-Marie U. Schröder, Arjen E. J. Wals, Kris van Koppen and others. As well as existing publications in Ukrainian publications, including articles written by Stanislav Dovgyi, Iryna Savchenko, Natalia Polihun, and others.
The research objective. To process and summarize the results of the survey of 1482 participants of the All-Ukrainian competition of environmental projects “Ecopohliad” for the years 2020 - 2021, 2021 - 2022 and 2022 - 2023.
The statement of basic materials. The results of a three-year survey of participants of the All-Ukrainian competition of environmental projects “Ecopohliad” are described in this article. We identify trends in using geographic information systems and remote sensing technologies in the educational process according to this survey. There is an analysis of the changes in the age profile of the participants and their self-reflection on the level of their environmental literacy. Some of the competition projects are described.
"THE DENIAL OF THE DENIAL" OF THE IDEAL IN THE SENSUAL ACTIVITY OF MAN-SOCIETY
Authors: Bohdan Pavlo
Published: June 2024

The research objective. We consider the problematic aspect in the formulation of the question of the ideal to be an attempt to imagine it as a totality, as an arbitrary transfer of subjective experience to the multidimensionality of the world. Its essence is understood through the prism of personal professional activity. We want to draw attention to the construct of the ideal, as a subjective reproduction of the objectively formed in the historical contexts of culture social pattern, which manifests itself in sensual and theoretical forms, remains integral and general. Existing forms of cultural universals in the substitution of the ideal are inseparably linked with the everyday norms of human existence.
The task of the article is to address the progressive and regressive forms of manifestation of the social ideal. We consider it necessary to formulate the task of reactualization of the constituting foundations of the ideal. This is possible in the way of understanding the artistic periods in terms of revealing the tendency to overcome the alienation from the individual of the sensual forms of the social ideal.
The statement of basic materials. The historically determined, objectively existing ideal does not depend on the existence, views of a particular individual. It is also not a totality of samples of professional (partial) practice. The ideal is universal, indirectly reasonable awareness of the monistic nature of existence. The sensual form of the ideal is considered by us from the point of view of the universal. The social ideal enables us to see the progressive and regressive tendencies in the development of practice. The criterion of progressiveness is the maximum liberation of the creative forces of the individual.
INTENTIONALITY AND FUNCTIONAL POTENTIAL OF ENGINEERING THINKING IN THE CONDITIONS OF MODERN MILITARY CHALLENGES
Authors: Vodovozov Illia
Published: June 2024

Actual scientific researches and issues analysis. The works of S. Winchester, E. Meyers, K. Mitch, K. Smythe, A. Ustundag, E. Chevikkan, E. Schatzberg, and others are devoted to the study of the role of technology and engineering in the human life space. Axiological and cultural-anthropological aspects of engineering thinking are considered in the works of Ukrainian researchers. First of all, this is the research of V. Voronkova, I. Goliad, I. Markus, and O. Shulga. Discussions about the responsible use of technology in the context of military operations and the creation and development of more peaceful innovations are of particular importance.
The statement of basic materials. Philosophy of technology is an important area of study because it examines the impact of technology on human existence, society, and the future of humanity. Technologies have a significant impact on human life and international relations, creating new challenges and fundamental questions about the ethics of their use, the responsibility of developers and users, as well as possible consequences for the future world. Issues related to establishing a balance between technological capabilities and general values of society require special attention.
The question of the genesis of the definition of engineering success requires special attention, which can conditionally occur depending on the context where the project is carried out. In peacetime, the success of this project is evaluated according to the criteria of efficiency, economy and innovation, since the task is to improve technologies, optimize processes and ensure sustainable development.
Engineering thinking in the conditions of a globalized world and longterm geopolitical claims must be ready for such challenges. First of all, it is about responsibility in ensuring the ethical and safe use of their developments. It is about supporting international efforts to control and regulate the export of military equipment, developing principles and standards for the ethical development and use of technologies for military purposes. In addition, engineers can play an active role in the development of technologies aimed at peaceful purposes and limiting the possibility of using technology in military conflicts.
The functional potential of engineering thinking in the creation of state-of-the-art technologies in the conditions of war is specified through the ability to develop, implement and adapt technical solutions capable of responding to complex military challenges. This covers the following aspects: innovativeness, adaptability, cooperation.