bluuebunny/crossref_metadata_2025 huggingface dataset:
Online Consumer Survey Comparing Different Front-of-Pack Labels in Greece
Authors: Kontopoulou Lamprini , Karpetas George , Fradelos Εvangelos C. , Papathanasiou Ioanna V. , Malli Foteini , Papagiannis Dimitrios , Mantzaris Dimitrios , Fialon Morgane , Julia Chantal , Gourgoulianis Konstantinos I.
Published: December 2021

According to the WHO, front-of-pack nutrition labeling provides simplified nutrition information in the form of symbols, colors or words that can help consumers understand the nutritional quality of food, thus leading them to healthier food choices. It is considered of the utmost importance to explore the knowledge and understanding of consumers about this form of nutrition labeling. The aim of this study was to investigate the understanding and perceptions of Greek consumers in response to five different front-of-pack nutrition labels (FoPLs): the Multiple Traffic Lights, Health Star Rating System, Guideline Daily Amounts, Warning Symbols and Nutri-Score. From April 2021 to June 2021, 1278 participants from Greece took part in an online survey where they were asked to rank three products according to their nutritional quality. The classification process was performed first without FoPLs and then with FoPLs. The ability to classify products according to their nutritional quality was evaluated with multinomial logistic regression models, and the Nutri-Score label presented greater improvements when compared to the GDA label for Greek consumers. The Nutri-Score seemed to better help the Greek consumers rank foods according to their nutritional value.
Metaproteomics Approach and Pathway Modulation in Obesity and Diabetes: A Narrative Review
Authors: Calabrese Francesco Maria , Porrelli Annalisa , Vacca Mirco , Comte Blandine , Nimptsch Katharina , Pinart Mariona , Pischon Tobias , Pujos-Guillot Estelle , De Angelis Maria
Published: December 2021

Low-grade inflammatory diseases revealed metabolic perturbations that have been linked to various phenotypes, including gut microbiota dysbiosis. In the last decade, metaproteomics has been used to investigate protein composition profiles at specific steps and in specific healthy/pathologic conditions. We applied a rigorous protocol that relied on PRISMA guidelines and filtering criteria to obtain an exhaustive study selection that finally resulted in a group of 10 studies, based on metaproteomics and that aim at investigating obesity and diabetes. This batch of studies was used to discuss specific microbial and human metaproteome alterations and metabolic patterns in subjects affected by diabetes (T1D and T2D) and obesity. We provided the main up- and down-regulated protein patterns in the inspected pathologies. Despite the available results, the evident paucity of metaproteomic data is to be considered as a limiting factor in drawing objective considerations. To date, ad hoc prepared metaproteomic databases collecting pathologic data and related metadata, together with standardized analysis protocols, are required to increase our knowledge on these widespread pathologies.
Dietary Intake in the Lifelines Cohort Study: Baseline Results from the Flower Food Frequency Questionnaire among 59,982 Participants
Authors: Baart A. Mireille , Brouwer-Brolsma Elske M. , Perenboom Corine W. M. , de Vries Jeanne H. M. , Feskens Edith J. M.
Published: December 2021

The role of nutrition in health and disease is well established. However, more research on this topic is needed to fill gaps in our current knowledge. The Lifelines cohort study, a large Dutch prospective cohort study, was established as a resource for international researchers, aiming to obtain insight into the aetiology of healthy ageing. The study started with 167,729 participants, covering three generations, aiming to follow them for thirty years. This article describes the habitual dietary intake, assessed using the Flower Food Frequency Questionnaire (FFQ), among Lifelines cohort study participants at baseline, stratified by sex and different categories of age, socioeconomic status (SES) and body mass index (BMI). A total of 59,982 adults (23,703 men and 36,279 women), who completed the Flower FFQ and reported plausible habitual dietary intake, were included in the analyses. Median daily energy intake was higher in men (2368 kcal) than in women (1848 kcal), as well as macronutrient intake. Energy and macronutrient intake decreased with increasing age and BMI categories; no differences were observed between SES categories. Intake of most micronutrients was higher in men than in women. Differences were observed between age categories, but not between SES and BMI categories. Food groups were consumed in different amounts by men and women; differences between age, SES and BMI categories were observed as well. The Lifelines cohort study provides extensive dietary intake data, which are generalisable to the general Dutch population. As such, highly valuable dietary intake data are available to study associations between dietary intake and the development of chronic diseases and healthy aging.
Socioeconomic, Eating- and Health-Related Limitations of Food Consumption among Polish Women 60+ Years: The ‘ABC of Healthy Eating’ Project
Authors: Hamulka Jadwiga , Frackiewicz Joanna , Stasiewicz Beata , Jeruszka-Bielak Marta , Piotrowska Anna , Leszczynska Teresa , Niedzwiedzka Ewa , Brzozowska Anna , Wadolowska Lidia
Published: December 2021

The study aimed at identifying the socioeconomic, eating- and health-related limitations and their associations with food consumption among Polish women 60+ years old. Data on the frequency of consumption of fruit, vegetables, dairy, meat, poultry, fish, legumes, eggs, water and beverages industrially unsweetened were collected with the Mini Nutritional Assessment (MNA®) and were expressed in the number of servings consumed per day or week. Three indexes: the Socioeconomic Status Index (SESI), the Eating-related Limitations Score (E-LS) and the Health-related Limitations Score (H-LS) were developed and applied. SESI was created on the base of two variables: place of residence and the self-reported economic situation of household. E-LS included: difficulties with self-feeding, decrease in food intake due to digestive problems, chewing or swallowing difficulties, loss of appetite, decrease in the feeling the taste of food, and feeling satiety, whereas H-LS included: physical function, comorbidity, cognitive function, psychological stress and selected anthropometric measurements. A logistic regression analysis was performed to assess the socioeconomic, eating-, and health-related limitations of food consumption. Lower socioeconomic status (vs. higher) was associated with a lower chance of consuming fruit/vegetables ≥ 2 servings/day (OR = 0.25) or consuming dairy ≥ 1 serving/day (OR = 0.32). The existence of multiple E-LS limitations (vs. few) was associated with a lower chance of consuming fruit/vegetables ≥ 2 servings/day (OR = 0.72), consuming dairy ≥ 1 serving/day (OR = 0.55) or consuming water and beverages industrially unsweetened ≥6 cups/day (OR = 0.56). The existence of multiple H-LS limitations was associated with a lower chance of consuming fruit/vegetables ≥ 2 servings/day (OR = 0.79 per 1 H-LS point increase) or consuming dairy ≥ 1 serving/day (OR = 0.80 per 1 H-LS point increase). Limitations found in the studied women were related to insufficient consumption of selected groups of food, which can lead to malnutrition and dehydration. There is a need for food policy actions, including practical educational activities, to eliminate barriers in food consumption, and in turn to improve the nutritional and health status of older women.
Prevalence of Cyclomodulin-Positive E. coli and Klebsiella spp. Strains in Mexican Patients with Colon Diseases and Antimicrobial Resistance
Authors: Canizalez-Roman Adrian , Reina-Reyes Juan E. , Angulo-Zamudio Uriel A. , Geminiano-Martínez Eloy E. , Flores-Carrillo Antonio F. , García-Matus Rolando R. , Valencia-Mijares Norma M. , Leon-Sicairos Nidia , Velazquez-Roman Jorge , Martínez-Villa Francisco A. , Tapia-Pastrana Gabriela
Published: December 2021

Colon diseases, such as colorectal cancer (CRC), are multifactor diseases that affect more than one million people per year; recently, the microbiota has been associated with an etiologic factor, specifically bacterial cyclomodulin positivity (CM+). Unfortunately, there are no studies from Mexico that detail the presence of bacterial CM+ in patients with colon diseases. We therefore performed a comprehensive study to investigate the associations and prevalence of cyclomodulin-positive Diarrheagenic E. coli (DEC), non-DEC, and Klebsiella spp. strains isolated from Mexican subjects with colon diseases. In this work, we analyzed 43 biopsies, 87 different bacteria were isolated, and E. coli was the most frequently noted, followed by Klebsiella spp., and Enterococcus spp. E. coli, non-DEC, and EPEC belonging to phylogroup B2 were the most prevalent. More than 80% of E. coli and Klebsiella were CM+. pks, cdt, cnf, and cif were identified. cdt was associated with non-DEC, cif and its combinations with EPEC, as well as cdt and psk with Klebsiella. Lastly, all the CM+ bacteria were resistant to at least one antibiotic (34% were MDR, and 48% XDR). In conclusion, the high prevalence of bacterial CM+ in colon disease patients suggests that these bacteria play an important role in the genesis of these diseases.
A Comprehensive Evaluation of Enterobacteriaceae Primer Sets for Analysis of Host-Associated Microbiota
Authors: Resendiz-Nava Carolina N. , Silva-Rojas Hilda V. , Rebollar-Alviter Angel , Rivera-Pastrana Dulce M. , Mercado-Silva Edmundo M. , Nava Gerardo M.
Published: December 2021

Enterobacteriaceae is one of the most important bacterial groups within the Proteobacteria phylum. This bacterial group includes pathogens, commensal and beneficial populations. Numerous 16S rRNA gene PCR-based assays have been designed to analyze Enterobacteriaceae diversity and relative abundance, and, to the best of our knowledge, 16 primer pairs have been validated, published and used since 2003. Nonetheless, a comprehensive performance analysis of these primer sets has not yet been carried out. This information is of particular importance due to the recent taxonomic restructuration of Enterobacteriaceae into seven bacterial families. To overcome this lack of information, the identified collection of primer pairs (n = 16) was subjected to primer performance analysis using multiple bioinformatics tools. Herein it was revealed that, based on specificity and coverage of the 16S rRNA gene, these 16 primer sets could be divided into different categories: Enterobacterales-, multi-family-, multi-genus- and Enterobacteriaceae-specific primers. These results highlight the impact of taxonomy changes on performance of molecular assays and data interpretation. Moreover, they underline the urgent need to revise and update the molecular tools used for molecular microbial analyses.
T908 Polymeric Micelles Improved the Uptake of Sgc8-c Aptamer Probe in Tumor-Bearing Mice: A Co-Association Study between the Probe and Preformed Nanostructures
Authors: Castelli Romina , Ibarra Manuel , Faccio Ricardo , Miraballes Iris , Fernández Marcelo , Moglioni Albertina , Cabral Pablo , Cerecetto Hugo , Glisoni Romina J. , Calzada Victoria
Published: December 2021

Aptamers are oligonucleotides that have the characteristic of recognizing a target with high affinity and specificity. Based on our previous studies, the aptamer probe Sgc8-c-Alexa647 is a promising tool for molecular imaging of PTK7, which is an interesting biomarker in cancer. In order to improve the delivery of this probe as well as create a novel drug delivery nanosystem targeted to the PTK7 receptor, we evaluate the co-association between the probe and preformed nanostructures. In this work, preformed pegylated liposomes (PPL) and linear and branched pristine polymeric micelles (PMs), based on PEO–PPO–PEO triblock copolymers were used: poloxamer F127® and poloxamines T1307® and T908®. For it, Sgc8-c-Alexa647 and its co-association with the different nanostructures was exhaustively analyzed. DLS analysis showed nanometric sizes, and TEM and AFM showed notable differences between free- and co-associated probe. Likewise, all nanosystems were evaluated on A20 lymphoma cell line overexpressing PTK7, and the confocal microscopy images showed distinctness in cellular uptake. Finally, the biodistribution in BALB/c mice bearing lymphoma-tumor and pharmacokinetic study revealed an encouraging profile for T908-probe. All data obtained from this work suggested that PMs and, more specifically T908 ones, are good candidates to improve the pharmacokinetics and the tumor uptake of aptamer-based probes.
Smart Lipid-Based Nanosystems for Therapeutic Immune Induction against Cancers: Perspectives and Outlooks
Authors: Fobian Seth-Frerich , Cheng Ziyun , ten Hagen Timo L. M.
Published: December 2021

Cancer immunotherapy, a promising and widely applied mode of oncotherapy, makes use of immune stimulants and modulators to overcome the immune dysregulation present in cancer, and leverage the host’s immune capacity to eliminate tumors. Although some success has been seen in this field, toxicity and weak immune induction remain challenges. Liposomal nanosystems, previously used as targeting agents, are increasingly functioning as immunotherapeutic vehicles, with potential for delivery of contents, immune induction, and synergistic drug packaging. These systems are tailorable, multifunctional, and smart. Liposomes may deliver various immune reagents including cytokines, specific T-cell receptors, antibody fragments, and immune checkpoint inhibitors, and also present a promising platform upon which personalized medicine approaches can be built, especially with preclinical and clinical potentials of liposomes often being frustrated by inter- and intrapatient variation. In this review, we show the potential of liposomes in cancer immunotherapy, as well as the methods for synthesis and in vivo progression thereof. Both preclinical and clinical studies are included to comprehensively illuminate prospects and challenges for future research and application.
Pharmacist-Facilitated Interactive E-Learning for Patients Newly Initiated on Warfarin: A Randomised Controlled Study
Authors: Young Joanne , Nalder Michelle J. , Gorelik Alexandra , Elliott Rohan A.
Published: December 2021

It is not known whether electronic-learning (e-learning) is effective for educating hospital inpatients about complex medications such as warfarin. This prospective randomised controlled study compared pharmacist-facilitated e-learning with standard pharmacist-delivered face-to-face education on patients’ or their unpaid carers’ knowledge of warfarin and satisfaction with warfarin education as well as the time that was spent by pharmacists in delivering warfarin education. Adult English-speaking patients (or their carers) who had been prescribed warfarin were randomised to receive standard pharmacist face-to-face education (control) or an e-learning module on a tablet device facilitated by a pharmacist (intervention). All of the participants received written warfarin information and were presented with the opportunity to ask any questions that they may have had to a pharmacist. Fifty-four participants completed the study (27 per group). The participants who received e-learning had median correct Oral Anticoagulation Knowledge (OAK) test scores of 85% compared to 80% for standard education (p = 0.14). The participants in both groups were satisfied with the information that they received. There was a trend towards pharmacists spending less time on warfarin education for the e-learning group than in the standard education group (25.5 vs. 33 min, respectively, p = 0.05). Education delivered via pharmacist-facilitated e-learning was non-inferior in terms of patient or carer warfarin knowledge compared to standard pharmacist-delivered education.
Naturalistic Moral Realism and Evolutionary Biology
Authors: Bloomfield Paul
Published: December 2021

Perhaps the most familiar understanding of “naturalism” derives from Quine, understanding it as a continuity of empirical theories of the world as described through the scientific method. So, it might be surprising that one of the most important naturalistic moral realists, Philippa Foot, rejects standard evolutionary biology in her justly lauded Natural Goodness. One of her main reasons for this is the true claim that humans can flourish (eudaimonia) without reproducing, which she claims cannot be squared with evolutionary theory and biology more generally. The present argument concludes that Foot was wrong to reject evolutionary theory as the empirical foundation of naturalized eudaimonist moral realism. This is based on contemporary discussion of biological function and evolutionary fitness, from which a definition of “eudaimonia” is constructed. This gives eudaimonist moral realism an empirically respectable foundation.
Mitigating the Negative Effect of Drought Stress in Oat (Avena sativa L.) with Silicon and Sulphur Foliar Fertilization
Authors: Kutasy Erika , Buday-Bódi Erika , Virág István Csaba , Forgács Fanni , Melash Anteneh Agezew , Zsombik László , Nagy Attila , Csajbók József
Published: December 2021

A field experiment was carried out in the 2020–2021 growing season, aiming at investigating the abiotic stress tolerance of oat (Avena sativa L.) with silicon and sulphur foliar fertilization treatments and monitoring the effect of treatments on the physiology, production and stress tolerance of winter oat varieties. In the Hungarian national list of varieties, six winter oat varieties were registered in 2020, and all of the registered varieties were sown in a small plot field experiment in Debrecen, Hungary. The drought tolerance of the oat could be tested, because June was very dry in 2021; the rainfall that month totaled 6 mm only despite a 30-year average of 66.5 mm, and the average temperature for the month was 3.2 °C higher than the 30-year average. Foliar application of silicon and sulphur fertilizers caused differences in the photosynthesis rate, total conductance to CO2, transpiration, water use efficiency, leaf area, chlorophyll content, carotenoid content, thousand kernel weight (TKW) and yield of winter oat. The application of silicon significantly increased the photosynthesis rate (16.8–149.3%), transpiration (5.4–5.6%), air–leaf temperature difference (16.2–43.2%), chlorophyll (1.0%) and carotenoid (2.5%) content. The yield increased by 10.2% (Si) and 8.0% (Si plus S), and the TKW by 3.3% (Si) and 5.0% (Si plus S), compared to the control plots. The plants in the control plots assimilated less CO2 while transpiring 1 m3 water more than in the Si, S or Si plus S fertilized plots. The effect of the silicon varied from 9.0 to 195.4% in water use efficiency (WUE) in the three development stages (BBCH52, BBCH65 and BBCH77). A lower leaf area index was measured in the foliar fertilized plots; even so, the yield was higher, compared to that from the control plots. Great variation was found in response to the foliar Si and S fertilization among winter oat varieties—in WUE, 2.0–43.1%; in total conductance to CO2, 4.9–37.3%; in leaf area, 1.6–34.1%. Despite the droughty weather of June, the winter oat varieties produced a high yield. The highest yield was in ‘GK Arany’ (7015.7 kg ha−1), which was 23.8% more than the lowest yield (‘Mv Kincsem’, 5665.6 kg ha −1). In the average of the treatments, the TKW increased from 23.9 to 33.9 g (41.8%). ‘Mv Hópehely’ had the highest TKW. Our results provide information about the abiotic stress tolerance of winter oat, which, besides being a good model plant because of its drought resistance, is an important human food and animal feed.
To Fight or to Grow: The Balancing Role of Ethylene in Plant Abiotic Stress Responses
Authors: Chen Hao , Bullock David A. , Alonso Jose M. , Stepanova Anna N.
Published: December 2021

Plants often live in adverse environmental conditions and are exposed to various stresses, such as heat, cold, heavy metals, salt, radiation, poor lighting, nutrient deficiency, drought, or flooding. To adapt to unfavorable environments, plants have evolved specialized molecular mechanisms that serve to balance the trade-off between abiotic stress responses and growth. These mechanisms enable plants to continue to develop and reproduce even under adverse conditions. Ethylene, as a key growth regulator, is leveraged by plants to mitigate the negative effects of some of these stresses on plant development and growth. By cooperating with other hormones, such as jasmonic acid (JA), abscisic acid (ABA), brassinosteroids (BR), auxin, gibberellic acid (GA), salicylic acid (SA), and cytokinin (CK), ethylene triggers defense and survival mechanisms thereby coordinating plant growth and development in response to abiotic stresses. This review describes the crosstalk between ethylene and other plant hormones in tipping the balance between plant growth and abiotic stress responses.
T6SS Accessory Proteins, Including DUF2169 Domain-Containing Protein and Pentapeptide Repeats Protein, Contribute to Bacterial Virulence in T6SS Group_5 of Burkholderia glumae BGR1
Authors: Kim Namgyu , Han Gil , Jung Hyejung , Lee Hyun-Hee , Park Jungwook , Seo Young-Su
Published: December 2021

Burkholderia glumae are bacteria pathogenic to rice plants that cause a disease called bacterial panicle blight (BPB) in rice panicles. BPB, induced by B. glumae, causes enormous economic losses to the rice agricultural industry. B. glumae also causes bacterial disease in other crops because it has various virulence factors, such as toxins, proteases, lipases, extracellular polysaccharides, bacterial motility, and bacterial secretion systems. In particular, B. glumae BGR1 harbors type VI secretion system (T6SS) with functionally distinct roles: the prokaryotic targeting system and the eukaryotic targeting system. The functional activity of T6SS requires 13 core components and T6SS accessory proteins, such as adapters containing DUF2169, DUF4123, and DUF1795 domains. There are two genes, bglu_1g23320 and bglu_2g07420, encoding the DUF2169 domain-containing protein in the genome of B. glumae BGR1. bglu_2g07420 belongs to the gene cluster of T6SS group_5 in B. glumae BGR1, whereas bglu_1g23320 does not belong to any T6SS gene cluster in B. glumae BGR1. T6SS group_5 of B. glumae BGR1 is involved in bacterial virulence in rice plants. The DUF2169 domain-containing protein with a single domain can function by itself; however, Δu1g23320 showed no attenuated virulence in rice plants. In contrast, Δu2g07420DUF2169 and Δu2g07420PPR did exhibit attenuated virulence in rice plants. These results suggest that the pentapeptide repeats region of the C-terminal additional domain, as well as the DUF2169 domain, is required for complete functioning of the DUF2169 domain-containing protein encoded by bglu_2g07420. bglu_2g07410, which encodes the pentapeptide repeats protein, composed of only the pentapeptide repeats region, is located downstream of bglu_2g07420. Δu2g07410 also shows attenuated virulence in rice plants. This finding suggests that the pentapeptide repeats protein, encoded by bglu_2g07410, is involved in bacterial virulence. This study is the first report that the DUF2169 domain-containing protein and pentapeptide repeats protein are involved in bacterial virulence to the rice plants as T6SS accessory proteins, encoded in the gene cluster of the T6SS group_5.
Comparative Study of Metabolomic Profile and Antioxidant Content of Adult and In Vitro Leaves of Aristotelia chilensis
Authors: Crisóstomo-Ayala Karina Andrea , Sabater-Jara Ana Belén , Pérez Manriquez Claudia , Ferreres Federico , Gil-Izquierdo Ángel , Pedreño Maria Ángeles , Hernández de la Torre Martha , Sanchez-Olate Manuel , Ríos Leal Darcy Graciela
Published: December 2021

This work aimed to identify the bioactive compounds present in adult maqui (Aristotelia chilensis) leaves from different stages of development and seasons of the year and compare them with leaves obtained from maqui plants grown in vitro. The qualitative and quantitative analysis of maqui leaf extracts by HPLC-DAD-ESI-MSn showed the presence of different polyphenolic compounds classified into galloyl and caffeoyl quinic acids, ellagitannins and ellagic acid- and flavonoid-derivatives. In general, the total phenolic content of the in vitro samples was higher than that of ex vitro samples, whereas the total flavonoid content was higher in winter basal leaves. Additionally, the analysis by HPLC-MS showed that the extract from spring basal leaves was enriched in quercetin, catechin, kaempferol and 3-caffeoyl quinic acids, while in the in vitro leaves extract, quercetin was not present. As regards lipophilic compounds identified by GC/MS, the samples of in vitro leaves showed a high presence of α-tocopherol and β-sitosterol. In contrast, the samples of adult leaves presented a hight level of linolenic and linoleic acids. These results suggest that maqui leaves could be an excellent source of antioxidants and lipophilic compounds for many industries, such as the nutraceutical and pharmaceutical industries.
Citrus Physiological and Molecular Response to Boron Stresses
Authors: Yang Lin-Tong , Pan Jun-Feng , Hu Neng-Jing , Chen Huan-Huan , Jiang Huan-Xin , Lu Yi-Bin , Chen Li-Song
Published: December 2021

Since the essentiality of boron (B) to plant growth was reported nearly one century ago, the implication of B in physiological performance, productivity and quality of agricultural products, and the morphogenesis of apical meristem in plants has widely been studied. B stresses (B deficiency and toxicity), which lead to atrophy of canopy and deterioration of Citrus fruits, have long been discovered in citrus orchards. This paper reviews the research progress of B stresses on Citrus growth, photosynthesis, light use efficiency, nutrient absorption, organic acid metabolism, sugar metabolism and relocation, and antioxidant system. Moreover, the beneficial effects of B on plant stress tolerance and further research in this area were also discussed.
Chitosan Membrane Containing Copaiba Oil (Copaifera spp.) for Skin Wound Treatment
Authors: Paranhos Sheila Barbosa , Ferreira Elisângela da Silva , Canelas Caio Augusto de Almeida , da Paz Simone Patrícia Aranha , Passos Marcele Fonseca , da Costa Carlos Emmerson Ferreira , da Silva Alisson Clay Rios , Monteiro Sergio Neves , Candido Verônica Scarpini
Published: December 2021

The interaction of copaiba oil in the polymer matrix of chitosan can produce a favorable synergistic effect and potentiate properties. Indeed, the bioactive principles present in copaiba oil have anti-inflammatory and healing action. In the present work, chitosan membranes containing different contents of copaiba oil copaíba (0.1, 0.5, 1.0 and 5.0% (v/v)) were for the first time investigated. The membranes were developed by the casting method and analyzed for their morphology, degree of intumescence, moisture content, contact angle, Scanning Electron Microscope, and X-ray diffractometry. These chitosan/copaiba oil porous membranes disclosed fluid absorption capacity, hydrophilic surface, and moisture. In addition, the results showed that chitosan membranes with the addition of 1.0% (v/v) of copaiba oil presented oil drops with larger diameters, around 123.78 μm. The highest fluid absorption indexes were observed in chitosan membranes containing 0.1 and 0.5% (v/v) of copaiba oil. In addition, the copaiba oil modified the crystalline structure of chitosan. Such characteristics are expected to favor wound treatment. However, biological studies are necessary for the safe use of chitosan/copaiba oil membrane as a biomaterial.
Chemically Dual-Modified Biochar for the Effective Removal of Cr(VI) in Solution
Authors: Yang Juanjuan , Song Yu , Yue Yan , Liu Wenfei , Che Quande , Chen Honglei , Ma Hongfang
Published: December 2021

Here, a dual-modification strategy using KMnO4 (potassium permanganate) and AlCl3·6H2O (aluminum chloride, hexahydrate) as co-modifiers to improve the Cr(VI) removal capacity of K2CO3 activated biochar is introduced. As a result, the dual-modified biochar with KMnO4 and AlCl3·6H2O has the calculated adsorption energy of −0.52 eV and −1.64 eV for HCrO4−, and −0.21 eV and −2.01 eV for Cr2O72−. The Al2O3 (aluminum oxide) and MnO (manganese oxide) embedded on the surface of dual-modified biochar bring more Cr(VI) absorption sites comparing to single-modified biochar, resulting in a maximum Cr(VI) saturated adsorption capacity of 152.86 mg g−1. The excellent removal performance is due to the synthetic effect of electrostatic attraction, reduction reaction, complexation reaction, and physical adsorption. The experimental results also indicated that the spontaneous adsorption process agreed well with the pseudo-second order and Langmuir models. This dual-modification strategy is not limited to the treatment of Cr(VI) with biochar, and may also be incorporated with the treatment of other heavy metals in aqueous environment.
Bisphenol A Release from Dental Composites and Resin-Modified Glass Ionomers under Two Polymerization Conditions
Authors: Tichy Antonin , Simkova Marketa , Vrbova Radka , Roubickova Adela , Duskova Michaela , Bradna Pavel
Published: December 2021

Bisphenol A (BPA)-based monomers are commonly contained in dental resin-based materials. As BPA is an endocrine disruptor, its long-term release from restorative composites and resin-modified glass ionomers (RM-GICs) under two polymerization conditions was measured in this study. Specimens of two conventional composites containing BPA-based monomers, two “BPA-free” composites, and two RM-GICs were polymerized from one side for 20 s at 1300 mW/cm2 or for 5 s at 3000 mW/cm2. The amounts of BPA released in artificial saliva and methanol after 1, 4, 9, 16, 35, 65, 130, and 260 days were measured using liquid chromatography–tandem mass spectrometry. The highest amounts of BPA were released from conventional composites, followed by RM-GICs, while the least was released from “BPA-free” composites. Amounts of released BPA were significantly higher in methanol and decreased gradually after the first day. Fast polymerization (5 s at 3000 mW/cm2) resulted in a significantly higher release of BPA after 1 day, but the effect of polymerization conditions was not significant overall. In conclusion, fast polymerization increased the initial release of BPA, but the released amounts were significantly lower than the current tolerable daily intake (4 μg/kg body weight/day) even in methanol, representing the worst-case scenario of BPA release.
Bacteriostatic and Cytotoxic Properties of Composite Material Based on ZnO Nanoparticles in PLGA Obtained by Low Temperature Method
Authors: Burmistrov Dmitriy E. , Simakin Alexander V. , Smirnova Veronika V. , Uvarov Oleg V. , Ivashkin Petr I. , Kucherov Roman N. , Ivanov Vladimir E. , Bruskov Vadim I. , Sevostyanov Mihail A. , Baikin Alexander S. , Kozlov Valery A. , Rebezov Maksim B. , Semenova Anastasia A. , Lisitsyn Andrey B. , Vedunova Maria V. , Gudkov Sergey V.
Published: December 2021

A low-temperature technology was developed for producing a nanocomposite based on poly (lactic-co-glycolic acid) and zinc oxide nanoparticles (ZnO-NPs), synthesized by laser ablation. Nanocomposites were created containing 0.001, 0.01, and 0.1% of zinc oxide nanoparticles with rod-like morphology and a size of 40–70 nm. The surface of the films from the obtained nanomaterial was uniform, without significant defects. Clustering of ZnO-NPs in the PLGA matrix was noted, which increased with an increase in the concentration of the dopant in the polymer. The resulting nanomaterial was capable of generating reactive oxygen species (ROS), such as hydrogen peroxide and hydroxyl radicals. The rate of ROS generation increased with an increase in the concentration of the dopant. It was shown that the synthesized nanocomposite promotes the formation of long-lived reactive protein species, and is also the reason for the appearance of a key biomarker of oxidative stress, 8-oxoguanine, in DNA. The intensity of the process increased with an increase in the concentration of nanoparticles in the matrix. It was found that the nanocomposite exhibits significant bacteriostatic properties, the severity of which depends on the concentration of nanoparticles. In particular, on the surface of the PLGA–ZnO-NPs composite film containing 0.001% nanoparticles, the number of bacterial cells was 50% lower than that of pure PLGA. The surface of the composite is non-toxic to eukaryotic cells and does not interfere with their adhesion, growth, and division. Due to its low cytotoxicity and bacteriostatic properties, this nanocomposite can be used as coatings for packaging in the food industry, additives for textiles, and also as a material for biomedicine.
Enhanced Heat Dissipation Performance of Automotive LED Lamps Using Graphene Coatings
Authors: Teng Tun-Ping , Chen Wei-Jen , Chang Chun-Hsin
Published: December 2021

Graphene heat-dissipating coating (GNHC) of 0.6 wt % GN concentration is utilized to promote the cooling performance of automotive light-emitting diode (LED) lamps. Three cases are studied as follows: Case 0 is the original automotive LED lamp as the baseline. Case 1 is to apply GNHC to reduce the thermal resistance of the junction surfaces between the components of automotive LED lamps. The aluminum fin radiator of Case 1 is further coated with GNHC on the surface that becomes Case 2. The spectrum, illuminance, power consumption, and surface temperature are measured at different ambient temperatures (Ta) to fully evaluate the feasibility of applying GNHC to improve cooling performance and the impacts on the related characteristics of automotive LED lamps. The results show that the maximum illuminance efficacy of Case 1 and Case 2 with high beam, irradiation angle of 0 degrees, and Ta of 80 °C is 11.03% and 8.70% higher than that of Case 0, respectively. The minimum temperature difference of heat dissipation path of Case 1 and Case 2 with high beam, irradiation angle of 90 degrees, and Ta of 80 °C is 6.41% and 5.33% lower than that of Case 0, respectively, indicating GNHC as a promising coating material for improving the cooling performance of automotive LED lamps.
Special Issue “Applications of Biosorption in Wastewater Treatment”
Authors: Vaamonde José Enrique Torres
Published: December 2021

The interest in the use of biosorption for the elimination of pollutants is because this technique is an efficient and environmentally friendly process, constituting an alternative to the so-called conventional treatment processes [...]
Kinetics Study of Hydrothermal Degradation of PET Waste into Useful Products
Authors: Čolnik Maja , Pečar Darja , Knez Željko , Goršek Andreja , Škerget Mojca
Published: December 2021

Kinetics of hydrothermal degradation of colorless polyethylene terephthalate (PET) waste was studied at two temperatures (300 °C and 350 °C) and reaction times from 1 to 240 min. PET waste was decomposed in subcritical water (SubCW) by hydrolysis to terephthalic acid (TPA) and ethylene glycol (EG) as the main products. This was followed by further degradation of TPA to benzoic acid by decarboxylation and degradation of EG to acetaldehyde by a dehydration reaction. Furthermore, by-products such as isophthalic acid (IPA) and 1,4-dioxane were also detected in the reaction mixture. Taking into account these most represented products, a simplified kinetic model describing the degradation of PET has been developed, considering irreversible consecutive reactions that take place as parallel in reaction mixture. The reaction rate constants (k1–k6) for the individual reactions were calculated and it was observed that all reactions follow first-order kinetics.
A Novel Flickering Multi-Target Joint Detection Method Based on a Biological Memory Model
Authors: Zhang Qian , Huo Weibo , Pei Jifang , Zhang Yongchao , Yang Jianyu , Huang Yulin
Published: December 2021

The robust target detection ability of marine navigation radars is essential for safe shipping. However, time-varying river and sea surfaces will induce target scattering changes, known as fluctuating characteristics. Moreover, the targets exhibiting stronger fluctuation disappear in some frames of the radar images, which is known as flickering characteristics. This phenomenon causes a severe decline in the detection performance of traditional detection methods. A biological memory model-based dynamic programming multi-target joint detection method was proposed to address this issue in this paper. Firstly, a global detection operator is used to discretize the multi-target state into multiple single-target states, achieving the discretization of numerous targets. Meanwhile, updating the formula of the memory weight merit function can strengthen the joint frame correlation of the flickering characteristics target. The progressive loop integral is utilized to update the target states to optimize the candidate target set. Finally, a two-stage threshold criterion is utilized to detect the target at different amplitude levels accurately. Simulation and experimental results are given to validate the assertion that the detection performance of the proposed method is greatly improved under a low SCR of 3-8 dB for multiple flickering target detection.
Assimilating C-Band Radar Data for High-Resolution Simulations of Precipitation: Case Studies over Western Sumatra
Authors: Zhu Bojun , Pu Zhaoxia , Putra Agie Wandala , Gao Zhiqiu
Published: December 2021

Accurate high-resolution precipitation forecasts are critical yet challenging for weather prediction under complex topography or severe synoptic forcing. Data fusion and assimilation aimed at improving model forecasts, as one possible approach, has gained increasing attention in past decades. This study investigates the influence of the observations from a C-band Doppler radar over the west coast of Sumatra on high-resolution numerical simulations of precipitation around its vicinity under the Madden–Julian oscillation (MJO) in January and February 2018. Cases during various MJO phases were selected for simulations with an advanced research version of the weather research and forecasting (WRF) model at a cloud-permitting scale (~3 km). A 3-dimensional variational (3DVAR) data assimilation method and a hybrid three-dimensional ensemble–variational data assimilation (3DEnVAR) method, based on the NCEP Gridpoint Statistical Interpolation (GSI) assimilation system, were used to assimilate the radar reflectivity and the radial velocity data. The WRF-simulated precipitation was validated with the Integrated Multi-satellitE Retrievals for GPM (IMERG) precipitation data, and the fractions skill score (FSS) was calculated in order to evaluate the radar data impacts objectively. The results show improvements in the simulated precipitation with hourly radar data assimilation 6 h prior to the simulations. The modifications with assimilation were validated through the observation departure and moist convection. It was found that forecast improvements are relatively significant when precipitation is more related to local-scale convection but rather small when the background westerly wind is strong under the MJO active phase. The additional simulation experiments, under a 1- or 2-day assimilation cycle, indicate better improvements in the precipitation simulation with 3DEnVAR radar assimilation than those with the 3DVAR method.
Analyzing the Application of X-Band Radar for Improving Rainfall Observation and Flood Forecasting in Yeongdong, South Korea
Authors: Yoon Seong-Sim , Lim Sang-Hun
Published: December 2021

The mountainous Yeongdong region of South Korea contains mountains over 1 km. Owing to this topographic blockage, the region has a low-density rain-gauge network, and there is a low-altitude (~1.5 km) observation gap with the nearest large S-band radar. The Korean government installed an X-band dual-polarization radar in 2019 to improve rainfall observations and to prevent hydrological disasters in the Yeongdong region. The present study analyzed rainfall estimates using the newly installed X-band radar to evaluate its hydrological applicability. The rainfall was estimated using a distributed specific differential phase-based technique for a high-resolution 75 m grid. Comparison of the rainfall estimates of the X-band radar and the existing rainfall information showed that the X-band radar was less likely to underestimate rainfall compared to the S-band radar. The accuracy was particularly high within a 10 km observation radius. To evaluate the hydrological applicability of X-band radar rainfall estimates, this study developed a rain-based flood forecasting method—the flow nomograph—for the Samcheok-osib stream, which is vulnerable to heavy rain and resultant floods. This graph represents the flood risk level determined by hydrological–hydraulic modeling with various rainfall scenarios. Rainfall information (X-band radar, S-band radar, ground rain gauge) was applied as input to the flow nomograph to predict the flood level of the stream. Only the X-band radar could accurately predict the actual high-risk increase in the water level for all studied rainfall events.
Deep Convolutional Neural Network for Rice Density Prescription Map at Ripening Stage Using Unmanned Aerial Vehicle-Based Remotely Sensed Images
Authors: Wei Lele , Luo Yusen , Xu Lizhang , Zhang Qian , Cai Qibing , Shen Mingjun
Published: December 2021

In this paper, UAV (unmanned aerial vehicle, DJI Phantom4RTK) and YOLOv4 (You Only Look Once) target detection deep neural network methods were employed to collected mature rice images and detect rice ears to produce a rice density prescription map. The YOLOv4 model was used for rice ear quick detection of rice images captured by a UAV. The Kriging interpolation algorithm was used in ArcGIS to make rice density prescription maps. Mature rice images collected by a UAV were marked manually and used to build the training and testing datasets. The resolution of the images was 300 × 300 pixels. The batch size was 2, and the initial learning rate was 0.01, and the mean average precision (mAP) of the best trained model was 98.84%. Exceptionally, the network ability to detect rice in different health states was also studied with a mAP of 95.42% in the no infection rice images set, 98.84% in the mild infection rice images set, 94.35% in the moderate infection rice images set, and 93.36% in the severe infection rice images set. According to the severity of rice sheath blight, which can cause rice leaves to wither and turn yellow, the blighted grain percentage increased and the thousand-grain weight decreased, the rice images were divided into these four infection levels. The ability of the network model (R2 = 0.844) was compared with traditional image processing segmentation methods (R2 = 0.396) based on color and morphology features and machine learning image segmentation method (Support Vector Machine, SVM R2 = 0.0817, and K-means R2 = 0.1949) for rice ear counting. The results highlight that the CNN has excellent robustness, and can generate a wide range of rice density prescription maps.
Automatic, Multiview, Coplanar Extraction for CityGML Building Model Texture Mapping
Authors: He Haiqing , Yu Jing , Cheng Penggen , Wang Yuqian , Zhu Yufeng , Lin Taiqing , Dai Guoqiang
Published: December 2021

Most 3D CityGML building models in street-view maps (e.g., Google, Baidu) lack texture information, which is generally used to reconstruct real-scene 3D models by photogrammetric techniques, such as unmanned aerial vehicle (UAV) mapping. However, due to its simplified building model and inaccurate location information, the commonly used photogrammetric method using a single data source cannot satisfy the requirement of texture mapping for the CityGML building model. Furthermore, a single data source usually suffers from several problems, such as object occlusion. We proposed a novel approach to achieve CityGML building model texture mapping by multiview coplanar extraction from UAV remotely sensed or terrestrial images to alleviate these problems. We utilized a deep convolutional neural network to filter out object occlusion (e.g., pedestrians, vehicles, and trees) and obtain building-texture distribution. Point-line-based features are extracted to characterize multiview coplanar textures in 2D space under the constraint of a homography matrix, and geometric topology is subsequently conducted to optimize the boundary of textures by using a strategy combining Hough-transform and iterative least-squares methods. Experimental results show that the proposed approach enables texture mapping for building façades to use 2D terrestrial images without the requirement of exterior orientation information; that is, different from the photogrammetric method, a collinear equation is not an essential part to capture texture information. In addition, the proposed approach can significantly eliminate blurred and distorted textures of building models, so it is suitable for automatic and rapid texture updates.
Distribution and Evolution of Supraglacial Lakes in Greenland during the 2016–2018 Melt Seasons
Authors: Hu Jinjing , Huang Huabing , Chi Zhaohui , Cheng Xiao , Wei Zixin , Chen Peimin , Xu Xiaoqing , Qi Shengliang , Xu Yifang , Zheng Yang
Published: December 2021

In recent decades, the melting of the Greenland Ice Sheet (GrIS) has become one of the major causes of global sea-level rise. Supraglacial lakes (SGLs) are typical hydrological features produced on the surface of the GrIS during the melt seasons. The existence and evolution of SGLs play an important role in the melting process of the ice sheet surface. To understand the distribution and recent changes of SGLs in Greenland, this study developed a random forest (RF) algorithm incorporating the texture and morphological features to automatically identify SGLs based on the Google Earth Engine (GEE) platform. Sentinel-2 imagery was used to map the SGLs inventory in Greenland during the 2016–2018 melt seasons and to explore the spatial and temporal variability characteristics of SGLs. Our results show changes in SGLs from 2016 to 2018, with the total area decreasing by ~1152.22 km2 and the number increasing by 1134; SGLs are mainly distributed in western Greenland (SW, CW, NW) and northeastern Greenland (NE), where the NE region has the largest number of observed SGLs and the largest SGL was with the surface area of 16.60 km2 (2016). SGLs were found to be most active in the area with the elevation of 800–1600 m and the slope of 0–5°, and showed a phenomenon of retreating to lower elevation areas and developing to steeper slope areas. Our work provided a method for rapid inventory of SGLs. This study will help monitor the mass balance of the GrIS and predict future rapid ice loss from Greenland.