bluuebunny/crossref_metadata_2025 huggingface dataset:
THE SAMPLING DISTRIBUTION OF LINKAGE DISEQUILIBRIUM
Authors: Golding G B
Published: January 2021

The probabilities of obtaining particular samples of gametes with two completely linked loci are derived. It is assumed that the population consists of N diploid, randomly mating individuals, that each of the two loci mutate according to the infinite allele model at a rate µ and that the population is at equilibrium. When 4Nµ is small, the most probable samples of gametes are those that segregate only two alleles at either locus. The probabilities of various samples of gametes are discussed. The results show that most samples with completely linked loci have either a very small or a very large association between the alleles of each locus. This causes the distribution of linkage disequilibrium to be skewed and the distribution of the correlation coefficient to be bimodal. The correlation coefficient is commonly used as a test statistic with a chi square distribution and yet has a bimodal distribution when the loci are completely linked. Thus, such a test is not likely to be accurate unless the rate of recombination between the loci and/or the effective population size are sufficiently large enough so that the loci can be treated as unlinked.
TEMPERATURE-SENSITIVE LETHAL MUTATIONS ON YEAST CHROMOSOME I APPEAR TO DEFINE ONLY A SMALL NUMBER OF GENES
Authors: Kaback David B , Oeller Paul W , Steensma H Yde , Hirschman Janet , Ruezinsky Diane , Coleman Kevin G , Pringle John R
Published: January 2021

A method was developed for isolating large numbers of mutations on chromosome I of the yeast Saccharomyces cerevisiae. A strain monosomic for chromosome I (i.e., haploid for chromosome I and diploid for all other chromosomes) was mutagenized with either ethyl methanesulfonate or N-methyl-N'-nitro-N -nitrosoguanidine and screened for temperature-sensitive (Ts-) mutants capable of growth on rich, glucose-containing medium at 25° but not at 37°. Recessive mutations induced on chromosome I are expressed, whereas those on the diploid chromosomes are usually not expressed because of the presence of wild-type alleles on the homologous chromosomes. Dominant ts mutations on all chromosomes should also be expressed, but these appeared rarely. — Of the 41 ts mutations analyzed, 32 mapped on chromosome I. These 32 mutations fell into only three complementation groups, which proved to be the previously described genes CDC15, CDC24 and PYK1 (or CDC19). We recovered 16 or 17 independent mutations in CDC15, 12 independent mutations in CDC24 and three independent mutations in PYK1. A fourth gene on chromosome I, MAK16, is known to be capable of giving rise to a ts-lethal allele, but we recovered no mutations in this gene. The remaining nine mutations isolated using the monosomic strain appeared not to map on chromosome I and were apparently expressed in the original mutants because they had become homozygous or hemizygous by mitotic recombination or chromosome loss. — The available information about the size of chromosome I suggests that it should contain approximately 60-100 genes. However, our isolation in the monosomic strain of multiple, independent alleles of just three genes suggests that only a small proportion of the genes on chromosome I is easily mutable to give a Ts--lethal phenotype. — During these studies, we located CDC24 on chromosome I and determined that it is centromere distal to PYK1 on the left arm of the chromosome.
ETHYLNITROSOUREA MUTAGENESIS AND THE ISOLATION OF MUTANT ALLELES FOR SPECIFIC GENES LOCATED IN THE t REGION OF MOUSE CHROMOSOME 17
Authors: Bode Vernon C
Published: January 2021

Ethylnitrosourea mutagenesis of spermatogonia in male mice is very efficient and makes it practical to isolate new desired mutant alleles by subsequent progeny screening. This is demonstrated for three genes in the t region of chromosome 17. The first, a mutation designated t-int, interacts with the dominant mutation, T (Brachyury), to produce a tailless mouse. Previously, mutant alleles of the t-int gene were available only in t haplotypes, where they are part of a t chromatin block within which recombination with wild-type chromosomes is inhibited. In addition to t-int, new mutations at the quaking and tufted loci were obtained, as well as at several loci not on chromosome 17, e.g., an X-linked lethal that causes a mottled phenotype in the heterozygote and four new mutant W alleles on chromosome 5. In the experiment, an average of one fertilizing spermatozoan in 1500 was mutant at a given locus and an average of one male in five was able to sire mutants at that locus.
ADDITIVE BY ADDITIVE VARIANCE WITH INBREEDING AND LINKAGE
Authors: Cockerham C Clark
Published: January 2021

Two-locus coancestries, which provide the coefficients of the additive by additive component in the variance and covariance of relatives for a quantitative trait, were formulated generally in terms of two-locus probabilities of identity by descent for combinations of parental and recombinant gametes. Explicit expressions, with linkage, were developed for all relatives of self-fertilization, for selfed and outbred relatives and for full and half sibs from unrelated inbred parents. The relative effect of linkage on the two-locus coancestry decreases rapidly as inbreeding and relatedness of the relatives increase. It was concluded that the error caused by ignoring linkage would probably be small in the estimation of genetic variances or in the projection of selection response with self-fertilization.
ALLELIC NEGATIVE COMPLEMENTATION AT THE ABRUPTEX LOCUS OF DROSOPHILA MELANOGASTER
Authors: Portin Petter
Published: January 2021

The mutations of the Abruptex locus in Drosophila melanogaster fall into three categories. There are recessive lethal alleles and viable alleles. The latter can be divided into suppressors and nonsuppressors of Notch mutations. The recessive lethals are lethal in heterozygous combination with Notch. As a rule the recessive lethals are lethal also in heterozygous combination with the viable alleles. Heterozygous combinations of certain viable alleles are also lethal. In such heterozygotes, one heteroallele is a suppressor of Notch and the other is a nonsuppressor. Other heterozygous combinations of viable alleles are viable and have an Abruptex phenotype. The insertion of the wild allele of the Abruptex locus as an extra dose (carried by a duplication) into the chromosomal complement of the fly fully restores the viability of the otherwise lethal heterozygotes if two viable alleles are involved. The extra wild allele also restores the viability of heterozygotes in which a lethal and a suppressor allele are present. If, however, a lethal and a nonsuppressor are involved, the wild allele only partly restores the viability, and the effect of the wild allele is weakest if two lethal alleles are involved. It seems likely that of the viable alleles the suppressors of Notch are hypermorphic and the nonsuppressors are hypomorphic. The lethal alleles share properties of both types, and are possibly antimorphic mutations. It is suggested that the locus is responsible for a single function which, however, consists of two components. The hypermorphic mutations are defects of the one component and the hypomorphic mutations of the other. In heterozygotes their cumulative action leads to decreased viability. The lethal alleles are supposed to be defects of the function as a whole. The function controlled by the locus might be a regulative function.
SELECTION FOR HIGH ADULT BODY WEIGHT IN DROSOPHILA POPULATIONS WITH DIFFERENT STRUCTURES
Authors: Katz A J , Young S S Y
Published: January 2021

Selection for high adult body weight in Drosophila melanogaster was practiced for 18 generations in three selection lines. These lines were genetically similar and of equal size but different in population structure. One line represented a large mass-selected, random-mating population, while the other two lines simulated large populations that had been subdivided into partial isolates or demes. Mass selection and random mating occurred within each deme. These two subdivided lines were different only in the rate of effective migration among the demes (5% and 10%). Selection intensities of approximately 20% were applied to these populations. A fourth line served as a random mating control. Heritability of adult body weight in the base population was estimated to be 0.58± 0.22. The results indicate that significantly greater responses were achieved in the subdivided lines than in the large mass-selected line, in spite of the fact that larger selection differentials were applied to the latter. No significant differences in response were observed between the two subdivided lines. Wright (1930, 1931) postulated that selection would be most efficient in subdivided populations with limited interdeme migration. The present findings appear to support this theory.
Adaptive gene misregulation
Authors: Wagner Andreas
Published: January 2021

Because gene expression is important for evolutionary adaptation, its misregulation is an important cause of maladaptation. A misregulated gene can be incorrectly silent (“off”) when a transcription factor (TF) that is required for its activation does not binds its regulatory region. Conversely, a misregulated gene can be incorrectly active (“on”) when a TF not normally involved in its activation binds its regulatory region, a phenomenon also known as regulatory crosstalk. DNA mutations that destroy or create TF binding sites on DNA are an important source of misregulation and crosstalk. Although misregulation reduces fitness in an environment to which an organism is well-adapted, it may become adaptive in a new environment. Here, I derive simple yet general mathematical expressions that delimit the conditions under which misregulation can be adaptive. These expressions depend on the strength of selection against misregulation, on the fraction of DNA sequence space filled with TF binding sites, and on the fraction of genes that must be expressed for optimal adaptation. I then use empirical data from RNA sequencing, protein-binding microarrays, and genome evolution, together with population genetic simulations to ask when these conditions are likely to be met. I show that they can be met under realistic circumstances, but these circumstances may vary among organisms and environments. My analysis provides a framework in which improved theory and data collection can help us demonstrate the role of misregulation in adaptation. It also shows that misregulation, like DNA mutation, is one of life’s many imperfections that can help propel Darwinian evolution.
The Association Between Light Physical Activity and Cognition Among Adults: A Scoping Review
Authors: Erlenbach Emily , McAuley Edward , Gothe Neha P
Published: January 2021

Background

The physical and cognitive benefits of moderate-vigorous intensity physical activity (MVPA) for adults have been well documented. Recently, there has been increasing interest in the independent health benefits of light-intensity physical activity (LPA). This research has primarily focused on the relationship between LPA and morbidity and mortality risk, with few studies investigating cognitive associations. The purpose of this scoping review was to catalog existing evidence on the association between device-based or technologically measured LPA and cognition among adults, identify trends in the literature, and recommend future areas for research.

Methods

Six electronic databases were searched between January and June 2020. Forty published studies met the inclusion criteria, which included both healthy and clinical young and older adult populations. Among the 40 articles were 14 acute exercise studies, 4 randomized control trials (RCTs), 18 cross-sectional studies, and 4 longitudinal studies.

Results

7/14 (50%) acute, 3/4 (75%) RCT, 10/18 (56%) cross-sectional, and 2/4 (50%) longitudinal studies reported a significant, positive relationship between LPA and one or more cognitive outcomes. These heterogeneous findings can largely be attributed to the diverse study designs and populations, as well as the numerous assessments used to test the cognitive domains.

Conclusion

These collective findings suggest LPA may be a potential lifestyle intervention to improve cognition across adulthood. However, the inconsistent approaches used among these studies suggest a more concerted, unified scientific approach is needed to further understand the LPA-cognition relationship.
Significantly improving the quality of genome assemblies through curation
Authors: Howe Kerstin , Chow William , Collins Joanna , Pelan Sarah , Pointon Damon-Lee , Sims Ying , Torrance James , Tracey Alan , Wood Jonathan
Published: January 2021

Genome sequence assemblies provide the basis for our understanding of biology. Generating error-free assemblies is therefore the ultimate, but sadly still unachieved goal of a multitude of research projects. Despite the ever-advancing improvements in data generation, assembly algorithms and pipelines, no automated approach has so far reliably generated near error-free genome assemblies for eukaryotes. Whilst working towards improved datasets and fully automated pipelines, assembly evaluation and curation is actively used to bridge this shortcoming and significantly reduce the number of assembly errors. In addition to this increase in product value, the insights gained from assembly curation are fed back into the automated assembly strategy and contribute to notable improvements in genome assembly quality. We describe our tried and tested approach for assembly curation using gEVAL, the genome evaluation browser. We outline the procedures applied to genome curation using gEVAL and also our recommendations for assembly curation in a gEVAL-independent context to facilitate the uptake of genome curation in the wider community.
Fighting for black stone: extractive conflict, institutional change and peacebuilding in Sierra Leone
Authors: Johnson Mckenzie F
Published: January 2021

Environmental governance reform—especially in the minerals sector—has featured prominently in Sierra Leone's peacebuilding agenda. While reform has enhanced environmental governance capacity in ways that foster peace, it has also exacerbated conflict over the redistribution of extractive rights. This article examines one such conflict over tantalite in northern Sierra Leone. In the chiefdom of Sella Limba, violence erupted as local landowners and a multinational company utilized institutional hybridity—or the blending of informal–indigenous institutions with liberal reforms—to construct competing claims over mineral rights. The resulting uncertainty over the extractive ‘rules of the game’ accelerated conflict as stakeholders attempted to (re)negotiate the distributional consequences of institutional change in real time. International and national actors ultimately rejected hybrid institutional arrangements on the grounds that they distorted post-conflict reforms and undermined peace. Drawing on in-depth fieldwork, I retrace the conflict to provide an alternative perspective. I contend that institutional hybridity served as a necessary component of, rather than barrier to, peacebuilding because it 1) opened space for diverse political participation in post-conflict environmental governance and 2) promoted greater political accountability and integration. These outcomes have been theorized as ways in which environmental reform can facilitate post-conflict peace. This argument aims to advance environmental peacebuilding theory by examining the conditions under which environmental governance reform contributes to post-conflict peacebuilding.
The Risk of Later Diagnosis of Inflammatory Bowel Disease in Patients With Dermatological Disorders Associated With Inflammatory Bowel Disease
Authors: King Dominic , Chandan Joht Singh , Thomas Tom , Nirantharakumar Krishnarajah , Reulen Raoul C , Adderley Nicola J , Trudgill Nigel
Published: January 2021

Background

Dermatological conditions such as erythema nodosum (EN), pyoderma gangrenosum, Sweet’s syndrome, and aphthous stomatitis can occur with inflammatory bowel disease (IBD) and are considered dermatological extraintestinal manifestations (D-EIMs). Rarely, they may precede IBD. Other common conditions such as psoriasis have also been associated with IBD. This study examined the risk of a subsequent IBD diagnosis in patients presenting with a D-EIM.

Methods

A retrospective cohort study compared patients with D-EIMs and age-/sex-matched patients without D-EIMs. Hazard ratios (HRs) were adjusted for age, sex, body mass index, deprivation, comorbidity, smoking, loperamide use, anemia, and lower gastrointestinal symptoms. Logistic regression was used to produce a prediction model for the diagnosis of IBD within 3 years of EN diagnosis.

Results

We matched 7447 patients with D-EIMs (74% female; median age 38 years (interquartile ratio [IQR], 24-65 years) to 29,297 patients without D-EIMs. We observed 131 (1.8%) subsequent IBD diagnoses in patients with D-EIMs compared with 65 (0.2%) in those without D-EIMs. Median time to IBD diagnosis was 205 days (IQR, 44-661 days) in those with D-EIMs and 1594 days (IQR, 693-2841 days) in those without D-EIMs. The adjusted HR for a later diagnosis of IBD was 6.16 (95% confidence interval [CI], 4.53-8.37; P < 0.001), for ulcerative colitis the HR was 3.30 (95% CI, 1.98-5.53; P < 0.001), and for Crohn’s disease the HR was 8.54 (95% CI, 5.74-12.70; P < 0.001). Patients with psoriasis had a 34% increased risk of a subsequent IBD diagnosis compared with the matched control patients (HR, 1.34; 95% CI, 1.20-1.51; P < 0.001). We included 4043 patients with an incident EN diagnosis in the prediction model cohort, with 87 patients (2.2%) diagnosed with IBD within 3 years. The model had a bias-corrected c-statistic of 0.82 (95% CI, 0.78-0.86).

Conclusions

Patients with D-EIMs have a 6-fold increased risk of a later diagnosis of IBD. Younger age, smoking, low body mass index, anemia, and lower gastrointestinal symptoms were associated with an increased risk of diagnosis of IBD within 3 years in patients with EN.
Performance analysis of basin-type vertical multiple-effect diffusion solar still integrated with solar pond: energy, exergy and economical analysis
Authors: Dhindsa Gurprinder Singh , Mittal Madhup Kumar , Sokhal Gurpreet Singh , Sokhal Kamaljit Singh , Khalilpoor Nima , Emani Sampath
Published: January 2021

An experimental analysis was carried out with basin-type vertical multiple-effect diffusion solar still coupled with solar pond (BVMEDS-SP) to evaluate 24 hours overall performance of still by varying feed flow rate and temperature in addition to other affecting parameters. A solar pond was utilized to harness solar energy diurnal and the same energy was provided to basin water nocturnal in batch mode to produce distillate throughout the day. It can be seen that the daily energy and exergy performance of BVMEDS-SP having four effects was improved by 21.74% and 16.83%, respectively, when the feed flow rate was reduced from 0.32 g/m2/s to 0.13 g/m2/s. As the temperature of feed water was enhanced from 25.65°C to 32.4°C, the daily energy and exergy efficiency of BVMEDS-SP correspondingly increased by 41.76% and 46.62%. In addition, increasing the number of effects from 2 to 5 improved the overall energy and exergy efficiency to 93.15% and 171.80%, respectively. The cost analysis revealed that cost of distillate for 2-effect still was Rs. 4.64/kg and it reduced to Rs. 3.11/kg for 7-effect still. A productivity correlation was furthermore developed and estimated that the experimental database of current research was in good agreement with the error band of −5% to +10%.
Variability in the assessment of children’s primary healthcare in 30 European countries
Authors: Luzi daniela , Rocco Ilaria , Tamburis Oscar , Corso Barbara , Minicuci Nadia , Pecoraro Fabrizio
Published: January 2021

Background

The high variability in the types and number of measures adopted to evaluate childcare across European countries makes it necessary to investigate country practices to identify trends in setting national priorities in the assessment of child well-being.

Objective

This paper intends to investigate country practices under the lens of variability to explore possible trends in setting national priority in the evaluation of childcare. In particular, it analyses variability considering to what extent this depends on the tendency of adopting a broad vision (i.e. selecting measures for a larger variety of aspects) or whether this is influenced by the choice of adopting an in-depth approach (i.e. using more measures to analyse a specific aspect)

Methods

An ad hoc questionnaire was administered to a national expert in each country and yielded 352 measures. To analyse variability, the breadth in the number of aspects considered was explored using a convergence index, while the depth in the distribution of measures in each aspect was investigated by computing a coefficient of variation. Countries were grouped by adopting a hierarchical clustering approach.

Results

There is a high variability across countries in the selection of measures that cover different aspects of childcare. Preferences in the distribution of measures are significant even at the domain level and in countries that use a limited number of measures and become more evident at the category and sub-category levels. The statistical analysis clusters countries in four main groups and two outliers. The in-depth distribution of measures focused on a specific aspect shows a homogeneous pattern, with the identification of two main groups of countries.

Conclusions

A limited set of measures are shared across countries hampering a robust comparison of paediatric models. The selection of measures shows that the evaluation is closely related to national priorities as resulting from the number and types of measures adopted. Moreover, a range of a reasonable number of measures can be hypothesized to address the quality of childcare under a multi-dimensional perspective.
Intended and unintended changes in length of stay following reconfiguration of emergency care departments
Authors: Bogh Søren Bie , Fløjstrup Marianne , Möller Sören , Bech Mickael , Johnsen Søren Paaske , Henriksen Daniel Pilsgaard , Mogensen Christian Backer , Lassen Annmarie Touborg , Brabrand Mikkel
Published: January 2021

Background

The Danish health-care system has witnessed noticeable changes in the acute hospital care organization. The reconfiguration includes closing hospitals, centralizing acute care functions and investing in new buildings and equipment.

Objective

To examine the impact on the length of stay (LOS) and the proportion of overnight stays for hospitalized acute care patients.

Methods

This nationwide interrupted time series examined trend changes in LOS and overnight stay. Admissions were stratified based on admission time (weekdays/weekends and time of day), age and the level of co-morbidity

Results

In 2007–2016, the global average LOS declined 2.9% per year (adjusted time ratio [CI (confidence interval) 95%] 0.971 [0.970–0.971]). The reconfiguration was overall not associated with change in trend of LOS (time ratio [CI 95%] 1.001 [1.000–1.002]). When admissions were stratified for either weekdays or weekends, the reconfiguration was associated with reduction of the underlying downward trend for weekdays (time ratio [CI 95%] 1.004 [1.003–1.005]) and increased downward trend for weekend admissions (time ratio [CI 95%] 0.996 [0.094–0.098]). Admissions at night were associated with a 0.7% trend change in LOS (time ratio [CI 95%] 0.993 [0.991–0.996]). The reconfiguration was not associated with trend changes for overnight stays.

Conclusion

The nationwide reconfiguration of acute hospital care was overall not associated with change in trend for the registered LOS and no change in trend for overnight stays. However, the results varied according to hospitalization time, where admissions during weekends and nights after the reconfiguration were associated with shortened LOS.
Baseline Response, Monitoring, and Cross-Resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to Sodium Channel Blocker Insecticides in Brazil
Authors: Kaiser Ingrid S , Kanno Rubens H , Bolzan Anderson , Amaral Fernando S A , Lira Ewerton C , Guidolin Aline S , Omoto Celso
Published: January 2021

Spodoptera frugiperda (J.E. Smith) is one of the key cross-crop pests in Brazilian agroecosystems. Field-evolved resistance of S. frugiperda to some conventional insecticides and Bt proteins has already been reported. Thus, the use of insecticides with new mode of action such as sodium channel blockers (indoxacarb and metaflumizone) could be an important tool in insecticide resistance management (IRM) programs. To implement a proactive IRM, we conducted baseline response and monitoring to indoxacarb and metaflumizone in 87 field populations of S. frugiperda collected from major maize-growing regions of Brazil from 2017 to 2020, estimated the frequency of resistance alleles to indoxacarb, and evaluated cross-resistance of indoxacarb and metaflumizone to some selected insecticides and Bt proteins. Low variation in susceptibility to indoxacarb (4.6-fold) and metaflumizone (2.6-fold) was detected in populations of S. frugiperda in 2017. The frequency of the resistance allele to indoxacarb was 0.0452 (0.0382–0.0527 CI 95%), by using F2 screen method. The mean survival at diagnostic concentration, based on CL99, varied from 0.2 to 12.2% for indoxacarb and from 0.0 to 12.7% for metaflumizone, confirming high susceptibility of S. frugiperda to these insecticides in Brazil. No cross-resistance was detected between sodium channel blocker insecticides and other insecticides (organophosphate, pyrethroid, benzoylurea, spinosyn, and diamide) and Bt proteins. These findings showed that sodium channel blocker insecticides are important candidates to be exploited in IRM strategies of S. frugiperda in Brazil.
State Cost-Share Programs for Forest Landowners in the Southern United States: A Review
Authors: Chizmar Stephanie J , Parajuli Rajan , Bardon Robert , Cubbage Frederick
Published: January 2021

The largest concentration of state-level forest cost-share programs in the United States can be found in the southern states. Since the inception of the first programs in the 1970s, the state-level forest cost-share programs in the US South have acted as models for the rest of the country. Cost-share programs compensate landowners through direct reimbursements to address barriers such as limited owner capital and cash flow in the initial years of investment. Through a review of the literature and progress reports from southern state forestry agencies, we qualitatively assessed state-level cost-share programs and their status in the southern states. We identified the common themes in the literature related to cost-share programs: market, nonmarket, and landowners’ perceptions and knowledge. Many of the programs enacted between the 1970s and 1980s aimed to ensure a sustainable timber supply, a market good, from private forestlands. A few of the programs enacted more recently compensate landowners for nonmarket benefits such as forest health or soil and water conservation. Two of the nine available programs are practically inactive in recent years because of a lack of funding. We discuss current prospects regarding funding, partnerships, and broadening the focus of incentives to cover forest-based ecosystem services.

Study Implications

Regionally, cost-share programs in the US South differ in eligibility criteria, funding source and status, and resource management objectives. The majority of state-level cost-share programs in the US South were enacted 30 to 50 years ago. The first cost-share programs were designed to support a continued timber supply from private forestlands, but a few recent programs have expanded their objectives to protect forest health and soil and water quality. Forest-based ecosystem service markets, specifically reforestation to capture atmospheric carbon dioxide and provide clean air and water, have become more prevalent in recent years. Funding for forest commodity incentive programs is a continual challenge. New funding sources and new programs are crucial to meet demands for incentives for landowners to provide both timber and ecosystem services outputs.
Dapagliflozin elevates plasma high-density lipoprotein levels and influences visceral fat gene expression in streptozotocin-induced diabetes mellitus
Authors: Kralova Eva , Marusakova Margareta , Hadova Katarina , Krenek Peter , Klimas Jan
Published: January 2021

Objectives

Dapagliflozin (Dapa) could potentially be used to treat type 1 diabetes mellitus. We tested the hypothesis that it would influence blood lipid levels and visceral fat accumulation in a rodent diabetic model.

Methods

We used three groups of male Wistar rats: Controls, streptozotocin (STZ)-treated rats and STZ-treated orally with Dapa (STZ+Dapa), 10 mg/kg/day for six weeks. Blood glucose and serum lipids levels were determined. Plasma levels of lipases (hormone-sensitive lipase, HSL and lipoprotein lipase, LPL), adipokines (leptin and adiponectin) and proinflammatory cytokines [tumour necrosis factor-alpha (TNFα) and interleukin-6 (IL-6)] were determined by ELISA assays. mRNA levels in the perirenal fat were determined by real-time PCR.

Key findings

Dapa suppressed STZ-related hyperglycemia by 20% (P < 0.05) and increased serum HDL when compared to the controls and the STZ-only treated rats (both P < 0.05). STZ treatment caused elevations of other serum lipids that were resistant to Dapa treatment. Dapa treatment also increased both plasma and visceral fat mRNA levels of leptin, LPL and IL-6, while decreasing plasma and fat expressions of HSL and TNFα compared to the STZ-only treated rats (all P < 0.05).

Conclusions

Our results suggest that Dapa, in addition to its antidiabetic effect, also influences the function of adipose tissue which could be beneficial in the treatment of diabetes.
Subacromial pigmented villonodular synovitis: case report and review
Authors: Pereira Vitor Luis , Baldan Arthur Rodrigues , Andreoli Carlos Vicente , Belangero Paulo Santoro , de Castro Pochini Alberto , Ejnisman Benno
Published: January 2021

Pigmented villonodular synovitis (PVNS) is a non-neoplastic proliferative process that involves synovial tissue in the joints, tendon sheaths and bursae. It usually occurs in young adults, aged 20–50 years, is characteristically monoarticular and of slow progression. Clinical symptoms are nonspecific, and joint stiffness and pain are common in long-term cases. Shoulder PVNS is known to be extremely rare, especially when affecting the subacromial bursal region without joint involvement.

Magnetic resonance is the imaging modality of choice in PVNS and is useful for diagnosis, surgical planning and monitoring. Complete surgical synovectomy remains the treatment of choice, and all pathological synovial tissue should be removed. Patients who are not properly treated can progress to joint destruction.

We describe a case of PVNS in the subacromial bursa of a 15-year-old patient, with exuberant symptoms and 2 years of evolution, treated with extensive synovectomy and good clinical results in the 1-year follow-up.
The production of auxin by dying cells
Authors: Sheldrake A Rupert
Published: January 2021

In this review, I discuss the possibility that dying cells produce much of the auxin in vascular plants. The natural auxin, indole-3-acetic acid (IAA), is derived from tryptophan by a two-step pathway via indole pyruvic acid. The first enzymes in the pathway, tryptophan aminotransferases, have a low affinity for tryptophan and break it down only when tryptophan levels rise far above normal intracellular concentrations. Such increases occur when tryptophan is released from proteins by hydrolytic enzymes as cells autolyse and die. Many sites of auxin production are in and around dying cells: in differentiating tracheary elements; in root cap cells; in nutritive tissues that break down in developing flowers and seeds; in senescent leaves; and in wounds. Living cells also produce auxin, such as those transformed genetically by the crown gall pathogen. IAA may first have served as an exogenous indicator of the presence of nutrient-rich decomposing organic matter, stimulating the production of rhizoids in bryophytes. As cell death was internalized in bryophytes and in vascular plants, IAA may have taken on a new role as an endogenous hormone.
A photospheric and chromospheric activity analysis of the quiescent retrograde-planet host ν Octantis A
Authors: Ramm D J , Robertson P , Reffert S , Gunn F , Trifonov T , Pollard K , Cantalloube F
Published: January 2021

The single-lined spectroscopic binary ν Octantis provided evidence of the first conjectured circumstellar planet demanding an orbit retrograde to the stellar orbits. The planet-like behaviour is now based on 1437 radial velocities (RVs) acquired from 2001 to 2013. ν Oct’s semimajor axis is only 2.6 au with the candidate planet orbiting $\nu ~{\rm Oct\, A}$ about mid-way between. These details seriously challenge our understanding of planet formation and our decisive modelling of orbit reconfiguration and stability scenarios. However, all non-planetary explanations are also inconsistent with numerous qualitative and quantitative tests including previous spectroscopic studies of bisectors and line-depth ratios, photometry from Hipparcos and the more recent space missions TESS and Gaia (whose increased parallax classifies $\nu ~{\rm Oct\, A}$ closer still to a subgiant, ∼K1 IV). We conducted the first large survey of $\nu ~{\rm Oct\, A}$’s chromosphere: 198 $\rm Ca\,{\small II}$ H-line and 1160 $\rm {H}\, \alpha$ indices using spectra from a previous RV campaign (2009–2013). We also acquired 135 spectra (2018–2020) primarily used for additional line-depth ratios, which are extremely sensitive to the photosphere’s temperature. We found no significant RV-correlated variability. Our line-depth ratios indicate temperature variations of only ±4 K, as achieved previously. Our atypical $\rm Ca\,{\small II}$ analysis models the indices in terms of S/N and includes covariance significantly in their errors. The $\rm {H}\, \alpha$ indices have a quasi-periodic variability that we demonstrate is due to telluric lines. Our new evidence provides further multiple arguments realistically only in favour of the planet.
Venous Thromboembolism Anticoagulation Prophylaxis Timing in Patients Undergoing Craniotomy for Tumor
Authors: Algattas Hanna , Talentino Spencer E , Eichar Bradley , Williams Abraham A , Murphy Joseph M , Zhang Xiaoran , Garcia Ricardo Martinez , Newhouse David , Jaman Emade , Safonova Aleksandra , Fields Daryl , Chow Ian , Engh Jonathan , Amankulor Nduka M
Published: January 2021

BACKGROUND

Prophylactic anticoagulation helps prevent postoperative venous thromboembolism (VTE) and time to initiation postcraniotomy has relied on clinical judgment and practice patterns.

OBJECTIVE

To compare risks of postoperative VTE and hemorrhage among patients undergoing tumor resection with initiation of prophylactic anticoagulation on postoperative day 1 (POD1) vs POD2.

METHODS

Adult patients undergoing craniotomy for tumor between 2008 and 2018 were retrospectively reviewed. Outcomes were recorded from the Electronic medical record (EMR) including deep vein thrombosis (DVT), pulmonary embolism (PE), and hemorrhage.

RESULTS

Of a total of 1168 patients undergoing craniotomy, 225 initiated anticoagulation on POD1 and 389 initiated on POD2. Of the 171 glioblastoma (GBM) cases, 64 initiated on POD1 and 107 on POD2. There were 9 DVTs (1.5%), 1 PE (0.20%), overall VTE rate of 1.6%, and 7 hemorrhagic complications (1.10%), 4 being clinically significant. The GBM cohort contained 4 DVTs (2.3%) and 3 hemorrhagic complications (1.80%). There was no increased risk of VTE or hemorrhage with anticoagulation initiated on POD2 compared to POD1 in either cohort. Multivariate analysis in both cohorts did not reveal a significant association between DVT, PE, or hemorrhagic complications with age, body mass index, GBM pathology, or extent of resection. Interestingly, glioma patients older than 70 with subtotal resection had a higher likelihood of suffering intracranial hemorrhage when anticoagulation was started on POD1 (odds ratio 12.98).

CONCLUSION

Risk of VTE or hemorrhagic complication did not significantly differ with prophylactic anticoagulation started on POD1 vs POD2. Early anticoagulation may certainly be considered in high risk cases; however, 1 group where risk may outweigh benefit is the elderly glioma population receiving a subtotal resection.
Robotic-Assisted Endoscopic Laminotomy: 2-Dimensional Operative Video
Authors: Li Yingda , Wang Michael Y
Published: January 2021

Endoscopy and robotics represent two emerging technologies within the field of spine surgery, the former an ultra-MIS approach minimizing the perioperative footprint and the latter leveraging accuracy and precision. Herein, we present the novel incorporation of robotic assistance into endoscopic laminotomy, applied to a 27-yr-old female with a large caudally migrated L4-5 disc herniation. Patient consent was obtained. Robotic guidance was deployed in (1) planning of a focussed laminotomy map, pivoting on a single skin entry point; (2) percutaneous targeting of the interlaminar window; and (3) execution of precision drilling, controlled for depth. Through this case, we illustrated the potential synergy between these 2 technologies in achieving precise bony removal tailored to the patient's unique pathoanatomy while simultaneously introducing safety mechanisms against human error and improving surgical ergonomics.
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The physicians consented to the publication of their images.
Spectrometer Using superconductor MIxer Receiver (SUMIRE) for laboratory submillimeter spectroscopy
Authors: Watanabe Yoshimasa , Chiba Yutaro , Sakai Takeshi , Tamanai Akemi , Suzuki Rikako , Sakai Nami
Published: January 2021

Recent spectroscopic observations by sensitive radio telescopes require accurate molecular spectral line frequencies to identify molecular species in a forest of lines detected. To measure rest frequencies of molecular spectral lines in the laboratory, an emission-type millimeter and submillimeter-wave spectrometer utilizing state-of-the-art radio-astronomical technologies is developed. The spectrometer is equipped with a 200 cm glass cylinder cell, a two-sideband (2SB) superconductor-insulator-superconductor (SIS) receiver in the 230 GHz band, and wide-band auto-correlation digital spectrometers. By using the four 2.5 GHz digital spectrometers, a total instantaneous bandwidth of the 2SB SIS receiver of 8 GHz can be covered with a frequency resolution of 88.5 kHz. Spectroscopic measurements of CH3CN and HDO are carried out in the 230 GHz band so as to examine the frequency accuracy, stability, sensitivity, as well as the intensity calibration accuracy of our system. As for the result of CH3CN, we confirm that the frequency accuracy for lines detected with sufficient signal-to-noise ratio is better than 1 kHz, when the high-resolution spectrometer having a channel resolution of 17.7 kHz is used. In addition, we demonstrate the capability of this system by spectral scan measurement of CH3OH from 216 GHz to 264 GHz. We assign 242 transitions of CH3OH, 51 transitions of 13CH3OH, and 21 unidentified emission lines for 295 detected lines. Consequently, our spectrometer demonstrates sufficient sensitivity, spectral resolution, and frequency accuracy for in-situ experimental-based rest frequency measurements of spectral lines for various molecular species.
S-nitrosoglutathione Reductase-Mediated Nitric Oxide Affects Axillary Buds Outgrowth of Solanum lycopersicum L. by Regulating Auxin and Cytokinin Signaling
Authors: Yan Yanyan , Shi Qinghua , Gong Biao
Published: January 2021

Auxin and cytokinin are two kinds of important phytohormones that mediate outgrowth of axillary buds in plants. How nitric oxide and its regulator of S-nitrosoglutathione reductase (GSNOR) take part in auxin and cytokinin signaling for controlling axillary buds outgrowth remains elusive. We investigated the roles of GSNOR during tomato axillary bud outgrowth by using physiological, biochemical and genetic approaches. GSNOR negatively regulated NO homeostasis. Suppression of GSNOR promoted axillary bud outgrowth by inhibiting the expression of FZY in both apical and axillary buds. Meanwhile, AUX1 and PIN1 were down-regulated in apical buds but up-regulated in axillary buds in GSNOR-suppressed plants. Thus, reduced IAA accumulation was shown in both apical buds and axillary buds of GSNOR-suppressed plants. GSNOR-mediated changes of NO and auxin affected cytokinin biosynthesis, transport, and signaling. And a decreased ratio of auxin: cytokinin was shown in axillary buds of GSNOR-suppressed plants, leading to bud dormancy breaking. We also found that the original NO signaling was generated by nitrate reductase (NR) catalyzing nitrate as substrate. NR-mediated NO reduced the GSNOR activity through S-nitrosylation of Cys-10, then induced a further NO burst, which played the above roles to promote axillary buds outgrowth. Together, GSNOR-mediated NO played important roles in controlling axillary buds outgrowth by altering the homeostasis and signaling of auxin and cytokinin in tomato plants.
Shaming and Stigmatizing Healthcare Workers in Japan During the COVID-19 Pandemic
Authors: Jecker Nancy S , Takahashi Shizuko
Published: January 2021

Stigmatization and sharming of healthcare workers in Japan during the coronavirus 2019 (COVID-19) pandemic reveal uniquely Japanese features. Seken, usually translated as ‘social appearance or appearance in the eyes of others,’ is a deep undercurrent woven into the fabric of Japanese life. It has led to providers who become ill with the SARS-CoV-2 virus feeling ashamed, while concealing their conditions from coworkers and public health officials. It also has led to healthcare providers being perceived as polluted and their children being told they were not welcome in schools. Although such experiences are not isolated to Japan and have appeared in other parts of the world, the cultural forces driving them in Japan are unique. Overcoming stigmatization and shaming of Japanese healthcare providers will require concerted efforts to understand cultural barriers and to view such practices as raising human rights issues affecting the safety and well-being of all.
On Trying to Leave Truth Alone
Authors: Zapero David
Published: January 2021

According to a certain conception of language, any sentence can, when used on an occasion, have any of indefinitely many truth-conditions. Such a conception of language gives us reason to think that the question of whether the notion of truth has a distinctive content cannot be settled by looking solely at the predication of truth. By focusing on the predicate ‘true’ when trying to determine the significance of the notion of truth, we may have been looking in the wrong place.
Can Cross-Border Funding Frictions Explain Financial Integration Reversals?
Authors: Akbari Amir , Carrieri Francesca , Malkhozov Aytek
Published: January 2021

We show that constraints on using leverage for foreign positions can act as an international investment barrier. Guided by an international CAPM with leverage constraints, we use observed stock prices to measure the variation in the magnitude and the implicit cost of such cross-border funding barriers. Our measure helps explain the dynamics of global market integration and, in particular, its reversals documented in the literature, but not explained by other international investment barriers. We confirm our results using alternative financial integration measures, international capital flows, and institutional portfolio holdings.
COMMITTED EFFECTIVE DOSE FROM NATURAL RADIONUCLIDES IN FARM-RAISED AND WILD CATFISH IN IBADAN, NIGERIA
Authors: Isinkaye M O , Ajilu F S , Ibikunle S B , Ajayi O S
Published: January 2021

There is extensive contamination of the aquatic environment when rivers and streams flow through areas of high human activities such as industrial cities and major towns. These contaminations may include enhanced background levels of naturally occurring radioactive materials resulting from rapid urbanization and other human activities. The natural radionuclides (40K, 238U (226Ra) and 232Th (228Ra)) contents of farm-raised and wild catfish in five fish farms and three major rivers within Ibadan metropolis have been studied using gamma spectrometry. The results indicate that the average committed effective doses from the ingestion of the two sets of catfishes are 104 ± 37 and 104 ± 32 μSv/y for farm-raised and wild catfish, respectively. It can be concluded that the consumption of the catfishes poses no immediate radiological hazards to the population of the study area.
Does investing in low-income urban neighborhoods improve sleep?
Authors: Dubowitz Tamara , Haas Ann , Ghosh-Dastidar Bonnie , Collins Rebecca L , Beckman Robin , Brooks Holliday Stephanie , Richardson Andrea S , Hale Lauren , Buysse Daniel J , Buman Matthew P , Troxel Wendy M
Published: January 2021

Study Objectives

Neighborhood disadvantage is associated with poor sleep, which may contribute to and exacerbate racial and socioeconomic health disparities. Most prior work has been cross-sectional and thus it has not been possible to estimate causal effects.

Methods

We leveraged a natural experiment opportunity in two low-income, predominantly African American Pittsburgh, PA neighborhoods, following a randomly selected cohort of households (n = 676) between 2013 and 2016. One of the neighborhoods received substantial public and private investments (housing, commercial) over the study period, while the other socio-demographically similar neighborhood received far fewer investments. Primary analyses used a difference-in-difference analysis based on neighborhood, to examine changes in actigraphy-assessed sleep duration, efficiency, and wakefulness after sleep onset (WASO), and self-reported sleep quality. Secondary analyses examined whether residents’ proximity to investments, regardless of neighborhood, was associated with changes in sleep outcomes.

Results

Resident sleep worsened over time in both neighborhoods with no significant differences among residents between the two neighborhoods. Secondary analyses, including covariate adjustment and propensity score weighting to improve comparability, indicated that regardless of neighborhood, those who lived in closer proximity to investments (<0.1 mile) were significantly less likely to experience decreases in sleep duration, efficiency, and quality, or increases in WASO, compared to those who lived farther away.

Conclusions

While we did not observe sleep differences among residents between neighborhoods, living closer to a neighborhood investment was associated with better sleep outcomes. Findings have relevance for public health and policy efforts focused on investing in historically disinvested neighborhoods.