bluuebunny/crossref_metadata_2025 huggingface dataset:
Systemic Inflammation and COVID-19 Mortality in Patients with Major Noncommunicable Diseases: Chronic Coronary Syndromes, Diabetes and Obesity
Authors: Buicu Andreea-Luciana , Cernea Simona , Benedek Imre , Buicu Corneliu-Florin , Benedek Theodora
Published: April 2021

COVID-19 is currently considered an inflammatory disease affecting the entire organism. In severe forms, an augmented inflammatory response leads to the fulminant “cytokine storm”, which may result in severe multisystemic end-organ damage. Apart from the acute inflammatory response, it seems that chronic inflammation also plays a major role in the clinical evolution of COVID-19 patients. Pre-existing inflammatory conditions, such as those associated with chronic coronary diseases, type 2 diabetes mellitus or obesity, may be associated with worse clinical outcomes in the context of COVID-19 disease. These comorbidities are reported as powerful predictors of poor outcomes and death following COVID-19 disease. Moreover, in the context of chronic coronary syndrome, the cytokine storm triggered by SARS-CoV-2 infection may favor vulnerabilization and rupture of a silent atheromatous plaque, with consequent acute coronary syndrome, leading to a sudden deterioration of the clinical condition of the patient. This review aims to present the current status of knowledge regarding the link between COVID-19 mortality, systemic inflammation and several major diseases associated with poor outcomes, such as cardiovascular diseases, diabetes and obesity.
The Role of the Rare Variants in the Genes Encoding the Alpha-Ketoglutarate Dehydrogenase in Alzheimer’s Disease
Authors: Csaban Dora , Pentelenyi Klara , Toth-Bencsik Renata , Illes Anett , Grosz Zoltan , Gezsi Andras , Molnar Maria Judit
Published: April 2021

There is increasing evidence that several mitochondrial abnormalities are present in the brains of patients with Alzheimer’s disease (AD). Decreased alpha-ketoglutarate dehydrogenase complex (αKGDHc) activity was identified in some patients with AD. The αKGDHc is a key enzyme in the Krebs cycle. This enzyme is very sensitive to the harmful effect of reactive oxygen species, which gives them a critical role in the Alzheimer and mitochondrial disease research area. Previously, several genetic risk factors were described in association with AD. Our aim was to analyze the associations of rare damaging variants in the genes encoding αKGDHc subunits and AD. The three genes (OGDH, DLST, DLD) encoding αKGDHc subunits were sequenced from different brain regions of 11 patients with histologically confirmed AD and the blood of further 35 AD patients. As a control group, we screened 134 persons with whole-exome sequencing. In all subunits, a one–one rare variant was identified with unknown significance based on American College of Medical Genetics and Genomics (ACMG) classification. Based on the literature research and our experience, R263H mutation in the DLD gene seems likely to be pathogenic. In the different cerebral areas, the αKGDHc mutational profile was the same, indicating the presence of germline variants. We hypothesize that the heterozygous missense R263H in the DLD gene may have a role in AD as a mild genetic risk factor.
Biomaterials for Drugs Nose–Brain Transport: A New Therapeutic Approach for Neurological Diseases
Authors: Cassano Roberta , Servidio Camilla , Trombino Sonia
Published: April 2021

In the last years, neurological diseases have resulted in a global health issue, representing the first cause of disability worldwide. Current therapeutic approaches against neurological disorders include oral, topical, or intravenous administration of drugs and more invasive techniques such as surgery and brain implants. Unfortunately, at present, there are no fully effective treatments against neurodegenerative diseases, because they are not associated with a regeneration of the neural tissue but rather act on slowing the neurodegenerative process. The main limitation of central nervous system therapeutics is related to their delivery to the nervous system in therapeutic quantities due to the presence of the blood–brain barrier. In this regard, recently, the intranasal route has emerged as a promising administration site for central nervous system therapeutics since it provides a direct connection to the central nervous system, avoiding the passage through the blood–brain barrier, consequently increasing drug cerebral bioavailability. This review provides an overview of the nose-to-brain route: first, we summarize the anatomy of this route, focusing on the neural mechanisms responsible for the delivery of central nervous system therapeutics to the brain, and then we discuss the recent advances made on the design of intranasal drug delivery systems of central nervous system therapeutics to the brain, focusing in particular on stimuli-responsive hydrogels.
Effect of the Croton rhamnifolioides Essential Oil and the Inclusion Complex (OEFC/β-CD) in Antinociceptive Animal Models
Authors: Oliveira Brito Pereira Bezerra Martins Anita , Correia de Oliveira Maria Rayane , Alcântara Isabel Sousa , Rodrigues Lindaiane Bezerra , Cesário Francisco Rafael Alves Santana , da Silva Maria Sanadia Alexandre , Castro Fyama Ferreira e , Nascimento Emmily Petícia do , Albuquerque Thaís Rodrigues de , Quintans Júnior Lucindo José , Araújo Adriano Antunes de Souza , Coutinho Henrique Douglas Melo , Menezes Irwin Rose Alencar de , Wanderley Almir Gonçalves
Published: April 2021

This study aims to evaluate the antinociceptive effect of the C. rhamnifolioides leaf essential oil (OEFC) and the β-cyclodextrin inclusion complex (COEFC) and investigate the pain signaling pathways involved in the antinociceptive response. The effects of the OEFC and COEFC on the central nervous system (CNS) were determined by open field and rota-rod assays, and the antinociceptive effect was evaluated via the acetic acid-induced abdominal contortions, formalin, and hot plate models. Swiss (Mus musculus) male mice (20–30 g) were used in both trials. The OEFC (200 mg/kg/v.o-orally) and COEFC (83.5 mg/kg/v.o.) did not present alterations in the CNS. The OEFC (25, 50, 100, and 200 mg/kg/vo.) and COEFC (8.35, 41.75, and 83.5 mg/kg/v.o.) demonstrated antinociceptive effects in the abdominal contortions, formalin, and hot plate tests. The OEFC (25 mg/kg/v.o.) and COEFC (8.35 mg/kg/v.o.) doses showed that the antinociceptive effect involves the activation of the opioid, cholinergic, and vanilloid systems, as well as the L-arginine/NO and α-2 adrenergic receptor pathways. The antinociceptive potential the OEFC and COEFC demonstrate possible alternatives for the therapy of pain. However, the COEFC presented more significant effects at lower doses than the isolated OEFC, where this action may be justified by the properties and advantages of the complexation.
An Application of p-Fibonacci Error-Correcting Codes to Cryptography
Authors: Bellini Emanuele , Marcolla Chiara , Murru Nadir
Published: April 2021

In addition to their usefulness in proving one’s identity electronically, identification protocols based on zero-knowledge proofs allow designing secure cryptographic signature schemes by means of the Fiat–Shamir transform or other similar constructs. This approach has been followed by many cryptographers during the NIST (National Institute of Standards and Technology) standardization process for quantum-resistant signature schemes. NIST candidates include solutions in different settings, such as lattices and multivariate and multiparty computation. While error-correcting codes may also be used, they do not provide very practical parameters, with a few exceptions. In this manuscript, we explored the possibility of using the error-correcting codes proposed by Stakhov in 2006 to design an identification protocol based on zero-knowledge proofs. We showed that this type of code offers a valid alternative in the error-correcting code setting to build such protocols and, consequently, quantum-resistant signature schemes.
Antimitochondrial Antibodies and Primary Biliary Cholangitis in Patients with Polymyalgia Rheumatica/Giant Cell Arteritis
Authors: Manzo Ciro , Maslinska Maria , Castagna Alberto , Hysa Elvis , Merante Alfonso , Milchert Marcin , Gravina Tiziana , Sargin Betul , Natale Maria , Ruberto Carmen , Ruotolo Giovanni
Published: April 2021

Background and Objectives: Laboratory liver abnormalities can be observed in patients affected with polymyalgia rheumatica (PMR) and/or giant cell arteritis (GCA), especially with a cholestatic pattern. The first objective of our review article is to discuss the potential link between antimitochondrial antibodies (AMA) and/or primary biliary cholangitis (PBC) and PMR/GCA, according to the evidences of literature. The second objective is to discuss the association of PMR/GCA with the other rheumatic diseases having PBC as a common manifestation. Materials and Methods: A literature search was performed on PubMed and Medline (OVID interface) using these terms: polymyalgia rheumatica, giant cell arteritis, antimitochondrial antibodies, primary biliary cholangitis, primary Sjogren’s syndrome, systemic sclerosis, and systemic lupus erythematosus. The search was restricted to all studies and case reports published in any language. Reviews, conference abstracts, comments, and non-original articles were excluded; however, each review’s reference list was scanned for additional publications meeting this study’s aim. When papers reported data partially presented in previous articles, we referred to the most recent published data. Results and Conclusions: Our literature search highlighted that cases reporting an association between AMA, PBC and PMR/GCA were very uncommon; AMA antigenic specificity had never been detected and biopsy-proven PBC was reported only in one patient with PMR/GCA. Finally, the association of PMR/GCA with autoimmune rheumatic diseases in which PBC is relatively common was anecdotal.
Photocytotoxic Activity of Ruthenium(II) Complexes with Phenanthroline-Hydrazone Ligands
Authors: Silva-Caldeira Priscila Pereira , Oliveira Junior Antônio Carlos Almendagna de , Pereira-Maia Elene Cristina
Published: April 2021

This paper reports on the synthesis and characterization of two new polypyridyl-hydrazone Schiff bases, (E)-N′-(6-oxo-1,10-phenanthrolin-5(6H)-ylidene)thiophene-2-carbohydrazide (L1) and (E)-N′-(6-oxo-1,10-phenanthrolin-5(6H)-ylidene)furan-2-carbohydrazide (L2), and their two Ru(II) complexes of the general formula [RuCl(DMSO)(phen)(Ln)](PF6). Considering that hydrazides are a structural part of severa l drugs and metal complexes containing phenanthroline derivatives are known to interact with DNA and to exhibit antitumor activity, more potent anticancer agents can be obtained by covalently linking the thiophene acid hydrazide or the furoic acid hydrazide to a 1,10-phenanthroline moiety. These ligands and the Ru(II) complexes were characterized by elemental analyses, electronic, vibrational, 1H NMR, and ESI-MS spectroscopies. Ru is bound to two different N-heterocyclic ligands. One chloride and one S-bonded DMSO in cis-configuration to each other complete the octahedral coordination sphere around the metal ion. The ligands are very effective in inhibiting cellular growth in a chronic myelogenous leukemia cell line, K562. Both complexes are able to interact with DNA and present moderate cytotoxic activity, but 5 min of UV-light exposure increases cytotoxicity by three times.
Traumatic Stress, Chronic Ethanol Exposure, or the Combination, Alter Cannabinoid System Components in Reward and Limbic Regions of the Mouse Brain
Authors: Piggott Veronica M. , Lloyd Scott C. , Matchynski James I. , Perrine Shane A. , Conti Alana C.
Published: April 2021

The cannabinoid system is independently affected by stress and chronic ethanol exposure. However, the extent to which co-occurrence of traumatic stress and chronic ethanol exposure modulates the cannabinoid system remains unclear. We examined levels of cannabinoid system components, anandamide, 2-arachidonoylglycerol, fatty acid amide hydrolase, and monoacylglycerol lipase after mouse single-prolonged stress (mSPS) or non-mSPS (Control) exposure, with chronic intermittent ethanol (CIE) vapor or without CIE vapor (Air) across several brain regions using ultra-high-performance liquid chromatography tandem mass spectrometry or immunoblotting. Compared to mSPS-Air mice, anandamide and 2-arachidonoylglycerol levels in the anterior striatum were increased in mSPS-CIE mice. In the dorsal hippocampus, anandamide content was increased in Control-CIE mice compared to Control-Air, mSPS-Air, or mSPS-CIE mice. Finally, amygdalar anandamide content was increased in Control-CIE mice compared to Control-Air, or mSPS-CIE mice, but the anandamide content was decreased in mSPS-CIE compared to mSPS-Air mice. Based on these data we conclude that the effects of combined traumatic stress and chronic ethanol exposure on the cannabinoid system in reward pathway regions are driven by CIE exposure and that traumatic stress affects the cannabinoid components in limbic regions, warranting future investigation of neurotherapeutic treatment to attenuate these effects.
Bioactivity Performance of Pure Mg after Plasma Electrolytic Oxidation in Silicate-Based Solutions
Authors: Husak Yevheniia , Michalska Joanna , Oleshko Oleksandr , Korniienko Viktoriia , Grundsteins Karlis , Dryhval Bohdan , Altundal Sahin , Mishchenko Oleg , Viter Roman , Pogorielov Maksym , Simka Wojciech
Published: April 2021

The biodegradable metals, including magnesium (Mg), are a convenient alternative to permanent metals but fast uncontrolled corrosion limited wide clinical application. Formation of a barrier coating on Mg alloys could be a successful strategy for the production of a stable external layer that prevents fast corrosion. Our research was aimed to develop an Mg stable oxide coating using plasma electrolytic oxidation (PEO) in silicate-based solutions. 99.9% pure Mg alloy was anodized in electrolytes contained mixtures of sodium silicate and sodium fluoride, calcium hydroxide and sodium hydroxide. Scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), contact angle (CA), Photoluminescence analysis and immersion tests were performed to assess structural and long-term corrosion properties of the new coating. Biocompatibility and antibacterial potential of the new coating were evaluated using U2OS cell culture and the gram-positive Staphylococcus aureus (S. aureus, strain B 918). PEO provided the formation of a porous oxide layer with relatively high roughness. It was shown that Ca(OH)2 was a crucial compound for oxidation and surface modification of Mg implants, treated with the PEO method. The addition of Ca2+ ions resulted in more intense oxidation of the Mg surface and growth of the oxide layer with a higher active surface area. Cell culture experiments demonstrated appropriate cell adhesion to all investigated coatings with a significantly better proliferation rate for the samples treated in Ca(OH)2-containing electrolyte. In contrast, NaOH-based electrolyte provided more relevant antibacterial effects but did not support cell proliferation. In conclusion, it should be noted that PEO of Mg alloy in silicate baths containing Ca(OH)2 provided the formation of stable biocompatible oxide coatings that could be used in the development of commercial degradable implants.
The Annealing of Acetylated Potato Starch with Various Substitution Degrees
Authors: Zięba Tomasz , Wilczak Aleksandra , Kobryń Justyna , Musiał Witold , Kapelko-Żeberska Małgorzata , Gryszkin Artur , Meisel Marta
Published: April 2021

This study aimed to determine the effect of “annealing” acetylated potato starch with a homogenous granule size and various degrees of substitution on the thermal pasting characteristics (DSC), resistance to amylases, rheology of the prepared pastes, swelling power and dynamics of drug release. A fraction of large granules was separated from native starch with the sedimentation method and acetylated with various doses of acetic anhydride (6.5, 13.0 or 26.0 26 cm3/100 g starch). The starch acetates were then annealed at slightly lower temperatures than their pasting temperatures. The annealing process caused an almost twofold increase in the resistance to amylolysis and a threefold increase in the swelling power of the modified starch preparations. The heat of phase transition decreased almost two times and the range of starch pasting temperatures over two times, but the pasting temperature itself increased by ca. 10 °C. The 40 g/100 g addition of the modified starch preparation decreased the rate of drug release from a hydrogel by ca. one-fourth compared to the control sample.
Physical, Chemical and Rheological Characterization of Tuber and Starch from Ceiba aesculifolia subsp. parvifolia
Authors: Suastegui-Baylón Lizette , Salazar Ricardo , Maldonado-Astudillo Yanik I. , Ramírez-Sucre Manuel O. , Arámbula-Villa Gerónimo , Flores-Casamayor Verónica , Jiménez-Hernández Javier
Published: April 2021

This work aimed to evaluate the physical, chemical and antioxidant properties of Ceiba aesculifolia subsp. parvifolia (CAP) tuber and determinate rheological, thermal, physicochemical and morphological properties of the starch extracted. The CAP tuber weight was 3.66 kg; the edible yield was 82.20%. The tuber presented a high hardness value (249 N). The content of carbohydrates (68.27%), crude fiber (15.61%) and ash (9.27%) from the isolated starch, reported in dry weight, were high. Phenolic compounds and flavonoid content of CAP tuber peel were almost 3-fold higher concerning the pulp. CAP tuber starch exhibited a pseudoplastic behavior and low viscosity at concentrations of 5–15%. Purity percentage and color parameters describe the isolated starch as high purity. Thermal characteristics indicated a higher degree of intermolecular association within the granule. Pasting properties describes starch with greater resistance to heat and shear. CAP tuber starch has X-ray diffraction patterns type A. The starch granules were observed as oval and diameters ranging from 5 to 30 µm. CAP tuber could be a good source of fiber and minerals, while its peel could be used for extracting bioactive compounds. Additionally, the starch separated from this tuber could be employed as a thickening agent in food systems requiring a low viscosity and subjected to high temperatures.
Health Beneficial Properties of Grapevine Seed Extract and Its Influence on Selected Biochemical Markers in the Blood, Liver and Kidneys of Rattus norvegicus
Authors: Sochorova Lenka , Baron Mojmir , Dadakova Katerina , Kasparovsky Tomas , Sochor Jiri
Published: April 2021

Cadmium (Cd) is a heavy metal that occurs in all areas of the environment, including the food chain. In the body, it causes oxidative stress by producing free radicals that are harmful to the cells. Grape seed extract (GSE) contains a wide range of biologically active components that help to neutralize the adverse effects of free radicals. In this study, the effects of GSE prepared form semi-resistant grapevine cultivar Cerason, which is rich in phenolics, on biochemical markers of brown rats exposed to the effects of cadmium were monitored. GSE increased the plasma antioxidant activity and, in the kidneys and the liver, Cd content was significantly lowered by GSE co-administration. Accordingly, the increase in creatinine content and alanine aminotransferase activity and the decrease of catalase and superoxide dismutase activities caused by cadmium were slowed down by GSE co-administration. The results of this work reveal that grape seed extract offers a protective effect against the intake of heavy metals into the organism.
The Presence of a Cyclohexyldiamine Moiety Confers Cytotoxicity to Pentacyclic Triterpenoids
Authors: Hoenke Sophie , Christoph Martin A. , Friedrich Sander , Heise Niels , Brandes Benjamin , Deigner Hans-Peter , Al-Harrasi Ahmed , Csuk René
Published: April 2021

Pentacyclic triterpenoids oleanolic acid, ursolic acid, betulinic acid, and platanic acid were acetylated and converted into several amides 9–31; the cytotoxicity of which has been determined in sulforhodamine B assays employing seral human tumor cell lines and nonmalignant fibroblasts. Thereby, a betulinic acid/trans-1,4-cyclohexyldiamine amide showed excellent cytotoxicity (for example, EC50 = 0.6 μM for HT29 colon adenocarcinoma cells).
Enhancement of β-Glucan Biological Activity Using a Modified Acid-Base Extraction Method from Saccharomyces cerevisiae
Authors: Mahmoud Amer Enas , Saber Saber H. , Abo Markeb Ahmad , Elkhawaga Amal A. , Mekhemer Islam M. A. , Zohri Abdel-Naser A. , Abujamel Turki S. , Harakeh Steve , Abd-Allah Elham A.
Published: April 2021

Beta glucan (β-glucan) has promising bioactive properties. Consequently, the use of β-glucan as a food additive is favored with the dual-purpose potential of increasing the fiber content of food products and enhancing their health properties. Our aim was to evaluate the biological activity of β-glucan (antimicrobial, antitoxic, immunostimulatory, and anticancer) extracted from Saccharomyces cerevisiae using a modified acid-base extraction method. The results demonstrated that a modified acid-base extraction method gives a higher biological efficacy of β-glucan than in the water extraction method. Using 0.5 mg dry weight of acid-base extracted β-glucan (AB extracted) not only succeeded in removing 100% of aflatoxins, but also had a promising antimicrobial activity against multidrug-resistant bacteria, fungi, and yeast, with minimum inhibitory concentrations (MIC) of 0.39 and 0.19 mg/mL in the case of resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa, respectively. In addition, AB extract exhibited a positive immunomodulatory effect, mediated through the high induction of TNFα, IL-6, IFN-γ, and IL-2. Moreover, AB extract showed a greater anticancer effect against A549, MDA-MB-232, and HepG-2 cells compared to WI-38 cells, at high concentrations. By studying the cell death mechanism using flow-cytometry, AB extract was shown to induce apoptotic cell death at higher concentrations, as in the case of MDA-MB-231 and HePG-2 cells. In conclusion, the use of a modified AB for β-glucan from Saccharomyces cerevisiae exerted a promising antimicrobial, immunomodulatory efficacy, and anti-cancer potential. Future research should focus on evaluating β-glucan in various biological systems and elucidating the underlying mechanism of action.
Anion–Anion Interactions in Aerogen-Bonded Complexes. Influence of Solvent Environment
Authors: Grabarz Anna , Michalczyk Mariusz , Zierkiewicz Wiktor , Scheiner Steve
Published: April 2021

Ab initio calculations are applied to the question as to whether a AeX5− anion (Ae = Kr, Xe) can engage in a stable complex with another anion: F−, Cl−, or CN−. The latter approaches the central Ae atom from above the molecular plane, along its C5 axis. While the electrostatic repulsion between the two anions prevents their association in the gas phase, immersion of the system in a polar medium allows dimerization to proceed. The aerogen bond is a weak one, with binding energies less than 2 kcal/mol, even in highly polar aqueous solvent. The complexes are metastable in the less polar solvents THF and DMF, with dissociation opposed by a small energy barrier.
Activation of Hair Cell Growth Factors by Linoleic Acid in Malva verticillata Seed
Authors: Ryu Hwa Sun , Jeong JiYeon , Lee Chun Mong , Lee Kwang Sik , Lee Jung-No , Park Sung-Min , Lee Yong-Moon
Published: April 2021

Hair loss by excessive stress from work and lifestyle changes has become a growing concern, particularly among young individuals. However, most drugs for alopecia impose a plethora of side effects. We have found the powerful impact of Malva verticillata seed extracts on alleviating hair loss. This study further isolated effective chemicals in M. verticillata seed extracts by liquid silica gel column chromatography. Under the screening for the growth rate (%) of human follicles dermal papilla cells (HFDPCs), we identified linoleic acid (LA) and oleic acid in n-hexane of M. verticillate (MH)2 fraction. LA treatment activated Wnt/β-catenin signaling and induced HFDPCs growth by increasing the expression of cell cycle proteins such as cyclin D1 and cyclin-dependent kinase 2. LA treatment also increased several growth factors, such as vascular endothelial growth factor, insulin-like growth factor-1, hepatocyte growth factor, and keratinocyte growth factor, in a dose-dependent manner. Besides, LA significantly inhibited Dickkopf-related protein expression (DKK-1), a primary alopecia signaling by dihydrotestosterone. Our findings suggest that LA treatment may alleviate a testosterone-induced signaling molecule and induces HFDPCs growth by activating Wnt/β-catenin signaling.
Glycan-Induced Protein Dynamics in Human Norovirus P Dimers Depend on Virus Strain and Deamidation Status
Authors: Dülfer Jasmin , Yan Hao , Brodmerkel Maxim N. , Creutznacher Robert , Mallagaray Alvaro , Peters Thomas , Caleman Carl , Marklund Erik G. , Uetrecht Charlotte
Published: April 2021

Noroviruses are the major cause of viral gastroenteritis and re-emerge worldwide every year, with GII.4 currently being the most frequent human genotype. The norovirus capsid protein VP1 is essential for host immune response. The P domain mediates cell attachment via histo blood-group antigens (HBGAs) in a strain-dependent manner but how these glycan-interactions actually relate to cell entry remains unclear. Here, hydrogen/deuterium exchange mass spectrometry (HDX-MS) is used to investigate glycan-induced protein dynamics in P dimers of different strains, which exhibit high structural similarity but different prevalence in humans. While the almost identical strains GII.4 Saga and GII.4 MI001 share glycan-induced dynamics, the dynamics differ in the emerging GII.17 Kawasaki 308 and rare GII.10 Vietnam 026 strain. The structural aspects of glycan binding to fully deamidated GII.4 P dimers have been investigated before. However, considering the high specificity and half-life of N373D under physiological conditions, large fractions of partially deamidated virions with potentially altered dynamics in their P domains are likely to occur. Therefore, we also examined glycan binding to partially deamidated GII.4 Saga and GII.4 MI001 P dimers. Such mixed species exhibit increased exposure to solvent in the P dimer upon glycan binding as opposed to pure wildtype. Furthermore, deamidated P dimers display increased flexibility and a monomeric subpopulation. Our results indicate that glycan binding induces strain-dependent structural dynamics, which are further altered by N373 deamidation, and hence hint at a complex role of deamidation in modulating glycan-mediated cell attachment in GII.4 strains.
Tunneling Spectroscopy for Electronic Bands in Multi-Walled Carbon Nanotubes with Van Der Waals Gap
Authors: Choi Dong-Hwan , Lee Seung Mi , Jeong Du-Won , Lee Jeong-O , Ha Dong Han , Bae Myung-Ho , Kim Ju-Jin
Published: April 2021

Various intriguing quantum transport measurements for carbon nanotubes (CNTs) based on their unique electronic band structures have been performed adopting a field-effect transistor (FET), where the contact resistance represents the interaction between the one-dimensional and three-dimensional systems. Recently, van der Waals (vdW) gap tunneling spectroscopy for single-walled CNTs with indium–metal contacts was performed adopting an FET device, providing the direct assignment of the subband location in terms of the current–voltage characteristic. Here, we extend the vdW gap tunneling spectroscopy to multi-walled CNTs, which provides transport spectroscopy in a tunneling regime of ~1 eV, directly reflecting the electronic density of states. This new quantum transport regime may allow the development of novel quantum devices by selective electron (or hole) injection to specific subbands.
Tuning the Acceptor Unit of Push–Pull Porphyrazines for Dye-Sensitized Solar Cells
Authors: Medina Diana-Paola , Fernández-Ariza Javier , Urbani Maxence , Sauvage Frédéric , Torres Tomás , Rodríguez-Morgade M. Salomé
Published: April 2021

A family of four push–pull porphyrazines of A3B type, where each unit A contains two peripheral propyl chains and the unit B is endowed with a carboxylic acid, were prepared. The carboxylic acid was attached to the β-position of the pyrrolic unit, either directly (Pz 10), or through cyanovinyl (Pz 11) and phenyl (Pz 7) groups. The fourth Pz (14) consisted in a pyrazinoporphyrazine wherein the dinitrogenated heterocycle provided intrinsic donor–acceptor character to the macrocycle and contained a carboxyphenyl substituent. The direct attachment of the carboxylic acid functions and their linkers to the porphyrazine core produces stronger perturbation on the electronic properties of the macrocycle, with respect to their connection through fused benzene or pyrazine rings in TT112 and 14, respectively. The HOMO and LUMO energies of the Pzs, which were estimated with DFT calculations, show little variation within the series, except upon introduction of the cyanovinyl spacer, which produces a decrease in both frontier orbital energetic levels. This effective interaction of cyanovinyl substitution with the macrocycle is also evidenced in UV/Vis spectroscopy, where a large splitting of the Q-band indicates strong desymmetrization of the Pz. The performance of the four Pzs as photosensitizers in DSSCs were also investigated.
Synthesis and Thermal Degradation Study of Polyhedral Oligomeric Silsesquioxane (POSS) Modified Phenolic Resin
Authors: Wang Degang , Ding Jie , Wang Bing , Zhuang Yingluo , Huang Zhixiong
Published: April 2021

In this paper, a new polyhedral oligomeric silsesquioxane containing a phenol group (POSS-Phenol) is prepared through the Michael addition reaction, which is added to the synthesis of phenolic resin as a functional monomer. Infrared spectroscopy (IR) is used to demonstrate the chemistry structure of the synthesized POSS modified phenolic resin. After introducing POSS into the resole, a comprehensive study is conducted to reveal the effects of POSS on the thermal degradation of phenolic resin. First, thermal degradation behaviors of neat phenolic resin and modified phenolic resin are carried out by thermogravimetric analysis (TGA). Then, the gas volatiles from thermal degradation are investigated by thermogravimetric mass spectrometry (TG-MS). Finally, the residues after thermal degradation are characterized by X-ray diffraction (XRD). The research indicates that POSS modified phenolic resin shows a better thermal stability than neat phenolic resin, especially at high temperatures under air atmosphere. On the one hand, the introduction of the POSS group can effectively improve the release temperature of oxygen containing volatiles. On the other hand, the POSS group forms silica at high temperatures under air, which can effectively inhibit the thermal oxidation of phenolic resin and make phenolic resin show a better high-temperature oxidation resistance.
Synthesis of the Microbial Polysaccharide Gellan from Dairy and Plant-Based Processing Coproducts
Authors: West Thomas P.
Published: April 2021

This review examines the production of the microbial polysaccharide gellan, synthesized by Sphingomonas elodea, on dairy and plant-based processing coproducts. Gellan is a water-soluble gum that structurally exists as a tetrasaccharide comprised of 20% glucuronic acid, 60% glucose and 20% rhamnose, for which various food, non-food and biomedical applications have been reported. A number of carbon and nitrogen sources have been tested to determine whether they can support bacterial gellan production, with several studies attempting to optimize gellan production by varying the culture conditions. The genetics of the biosynthesis of gellan has been explored in a number of investigations and specific genes have been identified that encode the enzymes responsible for the synthesis of this polysaccharide. Genetic mutants exhibiting overproduction of gellan have also been identified and characterized. Several dairy and plant-based processing coproducts have been screened to learn whether they can support the production of gellan in an attempt to lower the cost of synthesizing the microbial polysaccharide. Of the processing coproducts explored, soluble starch as a carbon source supported the highest gellan production by S. elodea grown at 30 °C. The corn processing coproducts corn steep liquor or condensed distillers solubles appear to be effective nitrogen sources for gellan production. It was concluded that further research on producing gellan using a combination of processing coproducts could be an effective solution in lowering its overall production costs.
Interaction between Different Kinds of Quantum Phase Transitions
Authors: Plastino Angel Ricardo , Ferri Gustavo Luis , Plastino Angelo
Published: April 2021

We employ two different Lipkin-like, exactly solvable models so as to display features of the competition between different fermion–fermion quantum interactions (at finite temperatures). One of our two interactions mimics the pairing interaction responsible for superconductivity. The other interaction is a monopole one that resembles the so-called quadrupole one, much used in nuclear physics as a residual interaction. The pairing versus monopole effects here observed afford for some interesting insights into the intricacies of the quantum many body problem, in particular with regards to so-called quantum phase transitions (strictly, level crossings).
Public Moral Discourse
Authors: Lovin Robin
Published: April 2021

Public moral discourse encompasses open discussions in which moral concepts of good and right are brought to bear on questions of public policy and on broader issues of basic rights and the goals and rules that guide social institutions. These public questions also raise practical, apologetic, and political concerns that are central to Christian ethics and moral theology. Public discourse frames legal and political understandings of religious freedom, and Christian ethics has a practical interest in ensuring that these choices do not limit Christian worship and formation or unduly restrict the institutional life of the church. Public discourse also engages apologetic theology in a moral task because the questions raised in public discourse involve conceptions of human good, human nature, and human community that have been discussed in Christian theology across the centuries. Christians have a distinctive understanding of persons in society that they hope to make effective, or at least to make understood, in a wider public discussion. Finally, public moral discourse gives rise to a moral responsibility for Christian participation in politics to create a public consensus on the creation of shared human goods.
Estimation of Tissue Attenuation from Ultrasonic B-Mode Images—Spectral-Log-Difference and Method-of-Moments Algorithms Compared
Authors: Brandner Dinah Maria , Cai Xiran , Foiret Josquin , Ferrara Katherine W. , Zagar Bernhard G.
Published: April 2021

We report on results from the comparison of two algorithms designed to estimate the attenuation coefficient from ultrasonic B-mode scans obtained from a numerical phantom simulating an ultrasound breast scan. It is well documented that this parameter significantly diverges between normal tissue and malignant lesions. To improve the diagnostic accuracy it is of great importance to devise and test algorithms that facilitate the accurate, low variance and spatially resolved estimation of the tissue’s attenuation properties. A numerical phantom is realized using k-Wave, which is an open source Matlab toolbox for the time-domain simulation of acoustic wave fields that facilitates both linear and nonlinear wave propagation in homogeneous and heterogeneous tissue, as compared to strictly linear ultrasound simulation tools like Field II. k-Wave allows to simulate arbitrary distributions, resolved down to single voxel sizes, of parameters including the speed of sound, mass density, scattering strength and to include power law acoustic absorption necessary for simulation tasks in medical diagnostic ultrasound. We analyze the properties and the attainable accuracy of both the spectral-log-difference technique, and a statistical moments based approach and compare the results to known reference values from the sound field simulation.
Leptospira interrogans Outer Membrane Protein-Based Nanohybrid Sensor for the Diagnosis of Leptospirosis
Authors: Verma Vivek , Kala Deepak , Gupta Shagun , Kumar Harsh , Kaushal Ankur , Kuča Kamil , Cruz-Martins Natália , Kumar Dinesh
Published: April 2021

Leptospirosis is an underestimated tropical disease caused by the pathogenic Leptospira species and responsible for several serious health problems. Here, we aimed to develop an ultrasensitive DNA biosensor for the rapid and on-site detection of the Loa22 gene of Leptospira interrogans using a gold nanoparticle–carbon nanofiber composite (AuN/CNF)-based screen-printed electrode. Cyclic voltammetry and electrochemical impedance were performed for electrochemical analysis. The sensitivity of the sensor was 5431.74 μA/cm2/ng with a LOD (detection limit) of 0.0077 ng/μL using cyclic voltammetry. The developed DNA biosensor was found highly specific to the Loa22 gene of L. interrogans, with a storage stability at 4 °C for 180 days and a 6% loss of the initial response. This DNA-based sensor only takes 30 min for rapid detection of the pathogen, with a higher specificity and sensitivity. The promising results obtained suggest the application of the developed sensor as a point of care device for the diagnosis of leptospirosis.
3D Sensors for Sewer Inspection: A Quantitative Review and Analysis
Authors: Bahnsen Chris H. , Johansen Anders S. , Philipsen Mark P. , Henriksen Jesper W. , Nasrollahi Kamal , Moeslund Thomas B.
Published: April 2021

Automating inspection of critical infrastructure such as sewer systems will help utilities optimize maintenance and replacement schedules. The current inspection process consists of manual reviews of video as an operator controls a sewer inspection vehicle remotely. The process is slow, labor-intensive, and expensive and presents a huge potential for automation. With this work, we address a central component of the next generation of robotic inspection of sewers, namely the choice of 3D sensing technology. We investigate three prominent techniques for 3D vision: passive stereo, active stereo, and time-of-flight (ToF). The Realsense D435 camera is chosen as the representative of the first two techniques wheres the PMD CamBoard pico flexx represents ToF. The 3D reconstruction performance of the sensors is assessed in both a laboratory setup and in an outdoor above-ground setup. The acquired point clouds from the sensors are compared with reference 3D models using the cloud-to-mesh metric. The reconstruction performance of the sensors is tested with respect to different illuminance levels and different levels of water in the pipes. The results of the tests show that the ToF-based point cloud from the pico flexx is superior to the output of the active and passive stereo cameras.
Indirect Temperature Measurement in High Frequency Heating Systems
Authors: Oskolkov Alexander , Bezukladnikov Igor , Trushnikov Dmitriy
Published: April 2021

One of the biggest challenges of fused deposition modeling (FDM)/fused filament fabrication (FFF) 3D-printing is maintaining consistent quality of layer-to-layer adhesion, and on the larger scale, homogeneity of material inside the whole printed object. An approach for mitigating and/or resolving those problems, based on the rapid and reliable control of the extruded material temperature during the printing process, was proposed. High frequency induction heating of the nozzle with a minimum mass (<1 g) was used. To ensure the required dynamic characteristics of heating and cooling processes in a high power (peak power > 300 W) heating system, an indirect (eddy current) temperature measurement method was proposed. It is based on dynamic analysis over various temperature-dependent parameters directly in the process of heating. To ensure better temperature measurement accuracy, a series-parallel resonant circuit containing an induction heating coil, an approach of desired signal detection, algorithms for digital signal processing and a regression model that determines the dependence of the desired signal on temperature and magnetic field strength were proposed. The testbed system designed to confirm the results of the conducted research showed the effectiveness of the proposed indirect measurement method. With an accuracy of ±3 °C, the measurement time is 20 ms in the operating temperature range from 50 to 350 °C. The designed temperature control system based on an indirect measurement method will provide high mechanical properties and consistent quality of printed objects.
Application of the Gaussian Mixture Model to Classify Stages of Electrical Tree Growth in Epoxy Resin
Authors: Mas’ud Abdullahi Abubakar , Sundaram Arunachalam , Ardila-Rey Jorge Alfredo , Schurch Roger , Muhammad-Sukki Firdaus , Bani Nurul Aini
Published: April 2021

In high-voltage (HV) insulation, electrical trees are an important degradation phenomenon strongly linked to partial discharge (PD) activity. Their initiation and development have attracted the attention of the research community and better understanding and characterization of the phenomenon are needed. They are very damaging and develop through the insulation material forming a discharge conduction path. Therefore, it is important to adequately measure and characterize tree growth before it can lead to complete failure of the system. In this paper, the Gaussian mixture model (GMM) has been applied to cluster and classify the different growth stages of electrical trees in epoxy resin insulation. First, tree growth experiments were conducted, and PD data captured from the initial to breakdown stage of the tree growth in epoxy resin insulation. Second, the GMM was applied to categorize the different electrical tree stages into clusters. The results show that PD dynamics vary with different stress voltages and tree growth stages. The electrical tree patterns with shorter breakdown times had identical clusters throughout the degradation stages. The breakdown time can be a key factor in determining the degradation levels of PD patterns emanating from trees in epoxy resin. This is important in order to determine the severity of electrical treeing degradation, and, therefore, to perform efficient asset management. The novelty of the work presented in this paper is that for the first time the GMM has been applied for electrical tree growth classification and the optimal values for the hyperparameters, i.e., the number of clusters and the appropriate covariance structure, have been determined for the different electrical tree clusters.
Alkali Iodide Deep Eutectic Solvents as Alternative Electrolytes for Dye Sensitized Solar Cells
Authors: Cruz Hugo , Pinto Ana Lucia , Jordão Noémi , Neves Luísa A. , Branco Luís C.
Published: April 2021

Different alkali deep eutectic solvents (DES), such as LiI:nEG, NaI:nEG, and KI:nEG, have been tested as electrolytes for dye sensitized solar cells (DSSCs). These DSSCs were prepared using pure DES or, alternatively, DES combined with different amounts of iodine (I2). The most important parameters, such as open circuit voltage (VOC), short circuit current density (JSC), fill factor (FF), and the overall conversion efficiency (η), were evaluated. Some DES seem to be promising candidates for DSSC applications, since they present higher VOC (up to 140 mV), similar FF values but less current density values, when compared with a reference electrolyte in the same experimental conditions. Additionally, electrochemical impedance spectroscopy (EIS) has been performed to elucidate the charge transfer and transport processes that occur in DSSCs. The values of different resistance (Ω·cm2) phenomena and recombination/relaxation time (s) for each process have been calculated. The best-performance was obtained for DES-based electrolyte, KI:EG (containing 0.5 mol% I2) showing an efficiency of 2.3%. The efficiency of this DES-based electrolyte is comparable to other literature systems, but the device stability is higher (only after seven months the performance of the device drop to 60%).
Toward Advancing Long-Term Outcomes of Kidney Transplantation with Artificial Intelligence
Authors: Castillo-Astorga Raúl , Sotomayor Camilo G.
Published: April 2021

After decades of pioneering advances and improvements, kidney transplantation is now the renal replacement therapy of choice for most patients with end-stage kidney disease (ESKD). Despite this success, the high risk of premature death and frequent occurrence of graft failure remain important clinical and research challenges. The current burst of studies and other innovative initiatives using artificial intelligence (AI) for a wide range of analytical and practical applications in biomedical areas seems to correlate with the same trend observed in publications in the kidney transplantation field, and points toward the potential of such novel approaches to address the aforementioned aim of improving long-term outcomes of kidney transplant recipients (KTR). However, at the same time, this trend underscores now more than ever the old methodological challenges and potential threats that the research and clinical community needs to be aware of and actively look after with regard to AI-driven evidence. The purpose of this narrative mini-review is to explore challenges for obtaining applicable and adequate kidney transplant data for analyses using AI techniques to develop prediction models, and to propose next steps in the field. We make a call to act toward establishing the strong collaborations needed to bring innovative synergies further augmented by AI, which have the potential to impact the long-term care of KTR. We encourage researchers and clinicians to submit their invaluable research, including original clinical and imaging studies, database studies from registries, meta-analyses, and AI research in the kidney transplantation field.
Induction of Robust and Specific Humoral and Cellular Immune Responses by Bovine Viral Diarrhea Virus Virus-Like Particles (BVDV-VLPs) Engineered with Baculovirus Expression Vector System
Authors: Wang Zhanhui , Liu Mengyao , Zhao Haoran , Wang Pengpeng , Ma Wenge , Zhang Yunke , Wu Wenxue , Peng Chen
Published: April 2021

Bovine viral diarrhea virus (BVDV) is an important animal pathogen that affects cattle. Infections caused by the virus have resulted in substantial economic losses and outbreaks of BVDV are reported globally. Virus-like particles (VLPs) are promising vaccine technology largely due to their safety and strong ability to elicit robust immune responses. In this study, we developed a strategy to generate BVDV-VLPs using a baculovirus expression vector system (BEVS). We were able to assemble BVDV-VLPs composed of dimerized viral proteins E2 and Erns, and the VLPs were spherical particles with the diameters of about 50 nm. Mice immunized with 15 μg of VLPs adjuvanted with ISA201 elicited higher levels of E2-specific IgG, IgG1, and IgG2a antibodies as well as higher BVDV-neutralizing activity in comparison with controls. Re-stimulation of the splenocytes collected from mice immunized with VLPs led to significantly increased levels of CD3+CD4+T cells and CD3+CD8+T cells. In addition, the splenocytes showed dramatically enhanced proliferation and the secretion of Th1-associated IFN-γ and Th2-associated IL-4 compared to that of the unstimulated control group. Taken together, our data indicate that BVDV-VLPs efficiently induced BVDV-specific humoral and cellular immune responses in mice, showing a promising potential of developing BVDV-VLP-based vaccines for the prevention of BVDV infections.