bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic and physiological determinants of lettuce partial resistance to Impatiens necrotic spot virus
Authors: Simko Ivan , Hasegawa Daniel K. , Peng Hui , Zhao Rebecca
Published: June 2023

Introduction

Impatiens necrotic spot virus (INSV) is a major pathogen currently threatening lettuce (Lactuca sativa L.) production in the coastal areas of California. The virus is transmitted by the western flower thrips (Frankliniella occidentalis Pergande).

Methods

We have tested a diversity panel of almost 500 lettuce accessions for disease incidence (DI) in 12 field experiments performed over 7 years. This set of accessions was also assessed for thrips feeding damage (TFD), the rate of plant development (PD), and the content of chlorophyll (SPAD) and anthocyanins (ACI) to determine their effect on resistance to INSV. In addition, recombinant inbred lines from two biparental mapping populations were also evaluated for DI in field experiments.

Results

The mean DI in 14 field experiments ranged from 2.1% to 70.4%. A highly significant difference in DI was observed among the tested accessions, with the overall lowest DI detected in the red color cultivars, Outredgeous Selection, Red Splash Cos, Infantry, Sweet Valentine, Annapolis, and Velvet. Multiple linear regression models revealed a small but significant effect (p < 0.005) of the four analyzed determinants on DI. Accessions with lower DI values had slower plant development (PD, r = 0.352), higher ACI content (r = −0.284), lower TFD (r = 0.198), and lower SPAD content (r = 0.125). A genome-wide association study revealed 13 QTLs for DI located on eight out of the nine lettuce chromosomes (the exception was chr. 8). The most frequently detected QTL (qINSV2.1) was located on chr. 2. Several of the QTLs for DI were in the same genomic areas as QTLs for PD, ACI, and SPAD. Additional three QTLs for DI on chr. 5 and 8 were identified using linkage mapping performed on two biparental mapping populations.

Conclusions

The work highlights the genetic basis of partial resistance to INSV and reveals the relationship between resistance, the host physiology, and the thrips vector. Results of this study are an important steppingstone toward developing cultivars with increased resistance against INSV.
Abscisic acid mimic-fluorine derivative 4 alleviates water deficit stress by regulating ABA-responsive genes, proline accumulation, CO2 assimilation, water use efficiency and better nutrient uptake in tomato plants
Authors: Jiménez-Arias David , Morales-Sierra Sarai , Suárez Emma , Lozano-Juste Jorge , Coego Alberto , Estevez Juan C. , Borges Andrés A. , Rodriguez Pedro L.
Published: June 2023

Water deficit represents a serious limitation for agriculture and both genetic and chemical approaches are being used to cope with this stress and maintain plant yield. Next-generation agrochemicals that control stomatal aperture are promising for controlling water use efficiency. For example, chemical control of abscisic acid (ABA) signaling through ABA-receptor agonists is a powerful method to activate plant adaptation to water deficit. Such agonists are molecules able to bind and activate ABA receptors and, although their development has experienced significant advances in the last decade, few translational studies have been performed in crops. Here, we describe protection by the ABA mimic-fluorine derivative 4 (AMF4) agonist of the vegetative growth in tomato plants subjected to water restriction. Photosynthesis in mock-treated plants is markedly impaired under water deficit conditions, whereas AMF4 treatment notably improves CO2 assimilation, the relative plant water content and growth. As expected for an antitranspirant molecule, AMF4 treatment diminishes stomatal conductance and transpiration in the first phase of the experiment; however, when photosynthesis declines in mock-treated plants as stress persists, higher photosynthetic and transpiration parameters are recorded in agonist-treated plants. Additionally, AMF4 increases proline levels over those achieved in mock-treated plants in response to water deficit. Thus water deficit and AMF4 cooperate to upregulate P5CS1 through both ABA-independent and ABA-dependent pathways, and therefore, higher proline levels are produced Finally, analysis of macronutrients reveals higher levels of Ca, K and Mg in AMF4- compared to mock-treated plants subjected to water deficit. Overall, these physiological analyses reveal a protective effect of AMF4 over photosynthesis under water deficit and enhanced water use efficiency after agonist treatment. In summary, AMF4 treatment is a promising approach for farmers to protect the vegetative growth of tomatoes under water deficit stress.
Geographic distribution of C4 species and its phylogenetic structure across China
Authors: Zhang Aiying , Yang Zhongjie , Zuo Yu , Ma Liang , Zhang Hanyu
Published: June 2023

Over the past fifty years, the distribution patterns of C4 species, across large spatial scales, are largely ignored. Here, we endeavored to examine patterns in the taxonomic and phylogenetic diversity of species with C4 photosynthetic pathways across the broad spatial extent of China and relate those to climatic gradients. We built a database of all plants with the C4 photosynthetic pathway in China. We analyzed the geographic distributions, taxonomic diversity, phylogenetic diversity, and phylogenetic structure of all C4 species, as well as the three families with the most C4 species (Poaceae, Amaranthaceae and Cyperaceae), and compared their values along temperature and precipitation gradients at two scales—the level of the province and at the 100 x 100 km grid cell. We found 644 C4 plants (belonging to 23 families 165 genera) in China, with Poaceae (57%), Amaranthaceae (17%), Cyperaceae (13%) accounting for the majority of species. Standardized effect size values of phylogenetic distances were negative overall, indicating that C4 species showed a phylogenetic clustering pattern. Southern China had the highest species richness and the highest degree of phylogenetic clustering. C4 tended to be more phylogenetically over-dispersed in regions with colder and/or drier climates, but more clustered in warmer and/or wetter climates. Patterns within individual families were more nuanced. The distribution of C4 species and its phylogenetic structure across China was constrained by temperature and precipitation. C4 species showed a phylogenetic clustering pattern across China, while different families showed more nuanced responses to climate variation, suggesting a role for evolutionary history.
Challenges in preserving the “good doctor” norm: physicians' discourses on changes to the medical logic during the initial wave of the COVID-19 pandemic
Authors: Härgestam Maria , Jacobsson Maritha , Bååthe Fredrik , Brulin Emma
Published: June 2023

Introduction

The COVID-19 pandemic was a tremendous challenge to the practice of modern medicine. In this study, we use neo-institutional theory to gain an in-depth understanding of how physicians in Sweden narrate how they position themselves as physicians when practicing modern medicine during the first wave of the pandemic. At focus is medical logic, which integrates rules and routines based on medical evidence, practical experience, and patient perspectives in clinical decision-making.

Methods

To understand how physicians construct their versions of the pandemic and how it impacted the medical logic in which they practice, we analyzed the interviews from 28 physicians in Sweden by discursive psychology.

Results

The interpretative repertoires showed how COVID-19 created an experience of knowledge vacuum in medical logic and how physicians dealt with clinical patient dilemmas. They had to find unorthodox ways to rebuild a sense of medical evidence while still being responsible for clinical decision-making for patients with critical care needs.

Discussion

In the knowledge vacuum occurring during the first wave of COVID-19, physicians could not use their common medical knowledge nor rely on published evidence or their clinical judgment. They were thus challenged in their norm of being the “good doctor”. One practical implication of this research is that it provides a rich empirical account where physicians are allowed to mirror, make sense, and normalize their own individual and sometimes painful struggle to uphold the professional role and related medical responsibility in the early phases of the COVID-19 pandemic. It will be important to follow how the tremendous challenge of COVID-19 to medical logic plays out over time in the community of physicians. There are many dimensions to study, with sick leave, burnout, and attrition being some interesting areas.
Effects of mobile-assisted reading materials on children’s L1 lexical development
Authors: Liu Xueli , Ni Chuanbin , Liu Yan
Published: June 2023

Despite the wide and extensive use of mobile-assisted devices, the effectiveness of children’s L1 learning with these mobile-assisted technologies has been less discussed. This study aims to explore the effects of mobile-assisted reading materials on Chinese children’s L1 vocabulary learning. We adopted a longitudinal and quasi-experimental design consisting of an experiment group using the mobile-assisted materials and a control group using the traditional paper materials, and took children’s lexical development as indexed by assessing the parameter, lexical diversity, in different testing times. The results showed that (1) children’s L1 vocabulary learning effectiveness of using mobile-assisted materials is as similar as that of using conventional paper materials in general, and (2) the changing patterns of children’s L1 lexical development using mobile-assisted materials in different testing times are various. Specifically speaking, (a) in the posttest 1 (the first month), compared with the traditional paper reading materials, the mobile-assisted reading materials have a facilitating effect on the primary school students’ L1 vocabulary learning; (b) in the posttest 2 (the second month), children’s vocabulary learning effectiveness is inhibited by the mobile-assisted reading materials; (c) in the delayed posttest (the fourth month), there is no difference in the learning effectiveness by these two different kinds of learning materials and the lexical diversity increases slowly but steadily. We analyzed the results from research-design factors and learner-related factors, hoping to shed light on children’s mobile-assisted language learning research.
Ascorbic acid is associated with favourable hormonal profiles among infertile males
Authors: Rastegar Panah Matineh , Tahir Irtaza , Garcia-Bailo Bibiana , Lo Kirk , Jarvi Keith , El-Sohemy Ahmed
Published: June 2023

Introduction

Infertility affects about 16% of North American couples, with the male factor contributing to ∼30% of cases. Reproductive hormones play an integral role in regulating the reproductive system and consequently, fertility. Oxidative stress reduces testosterone synthesis, and reduction in oxidative stress can improve hormone profiles. Ascorbic acid is a potent antioxidant that accounts for up to 65% of seminal antioxidant activity; however, its effects on reproductive hormones in humans are unknown.

Methods

The objective was to determine the association between serum ascorbic acid concentrations and male reproductive hormones. We conducted a cross-sectional study involving infertile males (n = 302) recruited from Mount Sinai Hospital, Toronto. Serum was analyzed for ascorbic acid, luteinizing hormone (LH), follicular stimulating hormone (FSH), total testosterone (TT), prolactin and estradiol. Statistical analyses included Spearman's rank correlations, linear regressions, logistic regressions, simple slope and Johnson-Neyman procedures.

Results

After adjusting for covariates, ascorbic acid was inversely associated with LH (P = 0.01). Ascorbic acid was positively associated with TT only among males over the age of 41.6 years (P = 0.01).

Discussion

Our findings show that ascorbic acid is associated with higher testosterone levels and improved androgenic status in infertile males, and some of the effects appear to be age dependent.
Utilising the learning in development research framework in a professional youth football club
Authors: O'Sullivan Mark , Vaughan James , Rumbold James L. , Davids Keith
Published: June 2023

Underpinned by an ecological dynamics rationale, the Learning in Development Research Framework (LDRF) has been suggested to introduce methodological possibilities to investigate and illuminate: (i) socio-cultural constraints within a sports organization or club, and (ii), a research gap on the need for a more contemporary framework to guide reliable ways of conducting investigations and designing practical applications. To provide a strong justification for the nature of the fieldwork and methods adopted, we present insights from a 3-year and 5-month study at a professional football club in Sweden that adapted the framework as a central feature of their Department of Methodology for player development. A phronetic iterative approach was employed to analyze the data. The findings highlight the nature of constraints acting over varied timescales, transcending contexts to manifest in other contexts (e.g., practice task designs), influencing events and experiences. This indicated a need to dampen (using probes) the influence of the pervasive organizational “control over context” approaches that were acting as “sticky” socio-cultural constraints, shaping the intentions (in session design) and attention (during practice and performance) of players and coaches. A practical implication is that the LDRF does not prescribe a universal solution to player development. Rather that it can guide how researchers, practitioners, clubs and organisations could challenge themselves to adapt strategies to design contemporary athlete development frameworks within their ecosystem.
Experimental Firing Test Campaign and Nozzle Heat Transfer Reconstruction in a 200 N Hybrid Rocket Engine with Different Paraffin-Based Fuel Grain Lengths
Authors: Cardillo Daniele , Battista Francesco , Gallo Giuseppe , Mungiguerra Stefano , Savino Raffaele
Published: June 2023

Firing test campaigns were carried out on a 200 N thrust-class hybrid rocket engine, using gaseous oxygen as an oxidizer and a paraffin-wax-based fuel. Different fuel grain lengths were adopted to extend the fuel characterization under different operating conditions, and to evaluate rocket performances and internal ballistics in the different configurations. In addition to data collected under a 220 mm propellant grain length, two further test campaigns were carried out considering 130 mm and 70 mm grain lengths. Two different injector types were adopted in the 130 mm configuration; in particular, a showerhead injection system was used with the aim to contain high-amplitude pressure oscillations observed during some firing tests in this engine configuration. Parameters such as the chamber pressure and temperature inside the graphite nozzle, space-averaged fuel regression rate and nozzle throat diameter were measured. The results allowed for the investigation of different issues related to hybrid rockets (e.g., fuel regression rate, engine performance, nozzle ablation under different conditions). The focus was mainly directed to the nozzle heat transfer, through the reconstruction of the convective heat transfer coefficient for different tests in the 70 mm grain length engine configuration. The reconstruction took advantage of the experimental data provided by the nozzle embedded thermocouple. Then, the experimental convective heat transfer coefficient was used to validate the results from some empirical correlations. The results showed significant differences between the experimental convective heat transfer coefficients when considering tests with different oxidizer mass flow rates. Furthermore, the predictions from the empirical correlations proved to be more reliable only in cases characterized by oxidizer-rich conditions.
Numerical Study on Convective Heat Transfer of Liquid Metal Gallium in Turbine Guide Vane
Authors: Zhang Zhe , Wu Zeyu , Luo Xiang , Liu Weitong
Published: June 2023

The traditional blade cooling method can no longer meet the requirements of high cooling efficiency in modern engines. In order to solve this cooling problem, this paper proposes cooling turbine guide blades based on liquid metal. The feasibility was preliminarily verified using a one-dimensional heat conduction model. Then, using a numerical method, we found that the cooling effect of liquid metal is much better than that of air cooling. The main reason for its good cooling effect is that the heat transfer coefficient of liquid metal reaches a magnitude of tens of thousands. Moreover, as the inlet temperature of the liquid metal decreases and the inlet Reynolds number increases, the liquid cooling effect becomes better. The definition of the heat transfer quality factor can reflect the reasons for the influence of the inlet temperature of the liquid metal.
Evaluation of Land Suitability for Olive (Olea europaea L.) Cultivation Using the Random Forest Algorithm
Authors: Ozalp Ayse Yavuz , Akinci Halil
Published: June 2023

Many large dams built on the Çoruh River have resulted in the inundation of olive groves in Artvin Province, Turkey. This research sets out to identify suitable locations for olive cultivation in Artvin using the random forest (RF) algorithm. A total of 575 plots currently listed in the Farmer Registration System, where olive cultivation is practiced, were used as inventory data in the training and validation of the RF model. In order to determine the areas where olive cultivation can be carried out, a land suitability map was created by taking into account 10 parameters including the average annual temperature, average annual precipitation, slope, aspect, land use capability class, land use capability sub-class, soil depth, other soil properties, solar radiation, and land cover. According to this map, an area of 53,994.57 hectares was detected as suitable for olive production within the study region. To validate the created model, the receiver operating characteristic (ROC) curve and the area under the ROC curve (AUC) were utilized. As a result, the AUC value was determined to be 0.978, indicating that the RF method may be successfully used in determining suitable lands for olive cultivation in particular, as well as crop-based land suitability research in general.
Effects of Drip Irrigation and Fertilization Frequency on Yield, Water and Nitrogen Use Efficiency of Medium and Strong Gluten Wheat in the Huang-Huai-Hai Plain of China
Authors: Hao Tianjia , Zhu Zixin , Zhang Yulu , Liu Shuai , Xu Yufan , Xu Xuexin , Zhao Changxing
Published: June 2023

Drip irrigation can reduce water and fertilizer use; however, the frequency of topdressing required for drip irrigation for wheat in the Huang-Huai-Hai region is still unclear. Through two continuous wheat season field experiments, yield related traits under traditional surface irrigation (border irrigation) and three drip fertilization frequencies (DF2, DF3, DF4, that was, topdressing water and fertilizer twice, three or four times in the same way during the growth period) of three wheat cultivars (Jimai 22, Jimai 20, Shiluan 02-1) were studied. Increasing the frequency of drip irrigation fertilization could prolong the time of high-level photosynthesis, increase the dry matter distribution amount (DMDA) of stems and leaves, and add the weight of 1000 grains; it could increase the DMDA and nitrogen distribution amount (NDA) of the stems, leaves, and grains of Jimai 22, forming higher harvest index (HI) and nitrogen harvest index (NHI), but could reduce the DMDA of the grains of Jimai 20 and Shiluan 02-1, increasing NDA, reducing the harvest index but forming a higher nitrogen harvest index. The increase in drip irrigation fertilization frequency can improve protein content, increase grain number per spike, decrease spike number, improve the yield of medium gluten wheat, and improve nitrogen partial productivity and water use efficiency, while strong gluten wheat has a decrease in yield, nitrogen partial productivity, and water use efficiency. In summary, medium gluten wheat is more suitable for higher fertilization frequency in the Huang-Huai-Hai wheat region, while strong gluten wheat is the opposite.
Agronomic Response to Irrigation and Biofertilizer of Peanut (Arachis hypogea L.) Grown under Mediterranean Environment
Authors: De Santis Michele Andrea , Campaniello Daniela , Tozzi Damiana , Giuzio Luigia , Corbo Maria Rosaria , Bevilacqua Antonio , Sinigaglia Milena , Flagella Zina
Published: June 2023

Peanut is a staple crop suitable for mechanized harvest and a source of plant proteins and fatty acids. It is widespread in Asia and North America, while there is limited cultivation in Europe despite potentially favorable climatic conditions. To test the adaptability of peanut in the Mediterranean area, a two-year field trial was carried out with one Spanish-type and one Virginia-type genotype cultivated under two water regimes (full irrigation and half irrigation supply). In order to test the response to fertilization management, three treatments were carried out, including an unfertilized control, a N-fertilized treatment, and a N-fertilized treatment inoculated with a commercial mixture of plant-growth promoting microorganisms, including two Bacillus species, Trichoderma and arbuscular mycorrhizal fungi (AMF). Microbiological soil analysis assessed the robustness of bacilli and their viability in soil. The Virginia-type genotype showed a better adaptability, with a positive response to irrigation and biofertilization. In particular, the inoculated treatment led to the highest agricultural crop water productivity, with important implications for sustainability. The impact of agronomic strategies was evaluated also in relation to storage proteins. The expression of 7s vicilin fraction showed a variability associated with water supply.
Mesenchymal Stem Cells: Generalities and Clinical Significance in Feline and Canine Medicine
Authors: Baouche Meriem , Ochota Małgorzata , Locatelli Yann , Mermillod Pascal , Niżański Wojciech
Published: June 2023

Mesenchymal stem cells (MSCs) are multipotent cells: they can proliferate like undifferentiated cells and have the ability to differentiate into different types of cells. A considerable amount of research focuses on the potential therapeutic benefits of MSCs, such as cell therapy or tissue regeneration, and MSCs are considered powerful tools in veterinary regenerative medicine. They are the leading type of adult stem cells in clinical trials owing to their immunosuppressive, immunomodulatory, and anti-inflammatory properties, as well as their low teratogenic risk compared with pluripotent stem cells. The present review details the current understanding of the fundamental biology of MSCs. We focus on MSCs’ properties and their characteristics with the goal of providing an overview of therapeutic innovations based on MSCs in canines and felines.
Empirical Evidence for the Rescue Effect from a Natural Microcosm
Authors: Lehtinen Richard M.
Published: June 2023

Ecological theory predicts that populations which receive immigrants are less vulnerable to extinction than those that do not receive immigrants (the “rescue effect”). A parallel but opposite process may also exist, where emigration increases the risk of local extinction (the “abandon-ship effect”). Using a natural microcosm of plant-specialist frogs from Madagascar, empirical evidence for both processes is provided. Populations receiving immigrants were less extinction-prone than those without immigration, and those populations losing individuals through emigration were more extinction-prone than those in which no emigration occurred. The number of immigrants and emigrants was also elevated and depressed (respectively) in patches that did not go extinct. These data provide some of the first definitive empirical evidence for the rescue effect and provide suggestive initial data on the abandon-ship effect. Both of these processes may be important to understanding the dynamics of populations.
Dietary Supplementation with Puerarin Improves Intestinal Function in Piglets Challenged with Escherichia coli K88
Authors: Zeng Yitong , Li Rui , Dong Yi , Yi Dan , Wu Tao , Wang Lei , Zhao Di , Zhang Yanyan , Hou Yongqing
Published: June 2023

The objective of this study was to investigate the effect of puerarin supplementation on the growth performance and intestinal function of piglets challenged with enterotoxigenic Escherichia coli (ETEC) K88. Twenty-four ternary crossbred piglets were randomly assigned to three treatment groups: control group, ETEC group (challenged with ETEC K88 on day 8), and ETEC + Puerarin group (supplemented with 5 mg/kg puerarin and challenged with ETEC K88 on day 8). All piglets were orally administered D-xylose (0.1 g/kg body weight) on day 10, and blood samples were collected after 1 h. Subsequently, piglets were killed and intestinal samples were collected for further analysis. The results showed that puerarin supplementation significantly decreased the adverse effects of ETEC K88-challenged piglets; significantly improved growth performance; increased the number of Bifidobacterium in the colon and Lactobacillus in the jejunum, cecum and colon; decreased the number of Escherichia coli in the jejunum and cecum; reduced the hydrogen peroxide content in the jejunum and myeloperoxidase activity in the jejunum and ileum; and increased the activities of catalase and superoxide dismutase in the jejunum and ileum. In addition, puerarin supplementation alleviated ETEC K88-induced intestinal injury in piglets, significantly downregulated the mRNA level of Interleukin-1β and upregulated the mRNA levels of intercellular cell adhesion molecule-1, myxovirus resistance protein 1, myxovirus resistance protein 2, and guanylate-binding protein-1 in the small intestine of piglets. In conclusion, dietary supplementation with puerarin could attenuate ETEC K88-induced intestinal injury by increasing the antioxidant and anti-inflammatory capacity and the number of beneficial intestinal bacteria in piglets.
Atipamezole Reverses Cardiovascular Changes Induced by High-Dose Medetomidine in Cats Undergoing Sedation for Semen Collection
Authors: Diggelmann Anna-Lea R. , Baron Toaldo Marco , Bektas Rima N. , Furthner Etienne , Reichler Iris M. , Kutter Annette P. N.
Published: June 2023

This study aimed at describing the change in echocardiographic variables after high-dose medetomidine and the reversal with atipamezole in six cats undergoing sedation for semen collection. Further cardiac Troponin I (cTnI) concentration and the effect of repeated sedation were assessed. Echocardiography was performed before and 20 min after sedation with 0.1 mg/kg medetomidine intramuscularly (IM) for urethral catheterisation. Prior to epididymectomy, S-ketamine was administered intravenously. Twenty minutes after reversal with 0.5 mg/kg atipamezole IM, the third echocardiography was performed. Sedation with medetomidine and reversal with atipamezole was repeated on day 7, 14, 21 and 28. Heart rate (HR) and rhythm were monitored throughout all sedations. On day 0 and 28 cTnI concentrations were measured before and after the procedure. After normality testing, the values were compared over time. The administration of medetomidine led to a marked reduction in HR, cardiac output and ventricular systolic function and a significant increase in left ventricular dimensions. Rhythm abnormalities, such as ventricular premature complexes and idioventricular rhythm, could be observed. The administration of atipamezole completely reversed sedation and the changes in haemodynamic variables. No significant increase in cTnI concentrations could be detected, although two out of six cats showed values above the reference range.
Nationwide Survey about the Occurrence of Aspergillosis in Captive Penguins in Zoos and Aquariums in Japan
Authors: Itoh Megumi , Matsumoto Naoya , Hagino Kyogo , Sawayama Nanako , Kuwayama Miki , Yamada Kazutaka , Toyotome Takahito
Published: June 2023

We surveyed the facilities that were members of the Japan Association of Zoos and Aquariums to clarify the incidence of aspergillosis, which is a major cause of death in captive penguins, and to discern effective preventive measures. Responses were obtained for 2910 penguins in 64 facilities; 73 penguins (2.5%) in 35 facilities had died from aspergillosis during the past 5 years from April 2016 to March 2021. Answers to questions about the rearing environment indicated that aspergillosis occurred significantly more often in facilities where penguins were reared outdoors, were in contact with soil, or were moved outside of the rearing enclosure. Answers to questions about their dead penguins indicated that 76% may have been at individual risk (e.g., young age, old age, molting period, and breeding season) and 54% were thought to be reared in uncomfortable environments (e.g., high temperature, high humidity). Aspergillosis may occur when individual risk factors and uncomfortable environmental factors are added to the risk factors of exposure to Aspergillus, such as the presence of soil. These conditions must be recognized as risk factors for aspergillosis, and appropriate preventive measures, such as avoiding penguin contact with the soil where Aspergillus is expected to be present, can minimize aspergillosis-related deaths.
Bearing Behavior of Cold-Formed Thick-Walled Steel Plates with a Single Bolt
Authors: Wang Lingling , Zhu Xin
Published: June 2023

This paper presents the results of an experimental and analytical study on the behavior of cold-formed thick-walled steel plates connected by a single bolt under double shear. Experimental studies were conducted on a total of 54 bolted connections fabricated from two thicknesses of cold-formed thick-walled steel plates, with nominal thicknesses of 6 mm and 10 mm. The effects of the end distance, edge distance, and steel plate thickness on the bolt bearing behavior were investigated. The codes of (modified) ANSI/AISC 360-22 and EN1993-1-8 were applied to predict the failure modes and bearing resistance of the connections, and the predictions were compared with those of the measurements. The studies show that fractures occurred on the shear planes of the specimens that failed because of the shear-out that occurred due to reduced ductility, which was caused by the cold forming process. The modified ANSI/AISC gives the most accurate predictions of the failure modes and bearing resistance. However, the bearing resistance will be overestimated if the reduction in the area of the shear planes caused by the occurrence of fractures is ignored in specimens with an end distance higher than 2.0d0, and a reduction factor is needed to calculate the bearing resistance of the connections.
Antimicrobial Activity and Biodiversity Study of a Homemade Vegetable Puree Treated with Antimicrobials from Paenibacillus dendritiformis
Authors: Mena Laura , Grande María José , Gálvez Antonio
Published: June 2023

Paenibacillus dendritiformis UJA2219 isolated from carrot produces broad-spectrum antibacterial activity. The aim of the present study was to determine the impact of partially-purified cell-culture extracts of strain UJA2219 on the microbial load and bacterial diversity of a homemade vegetable puree. The puree was challenged with an overnight culture of strain UJA2219 or with cultured broth extracts partially purified by cation exchange (CE) chromatography or reversed-phase (RP) chromatography and incubated for 7 days at temperatures of 4 °C or 25 °C. The best results were obtained at 25 °C with the RP extract, decreasing counts of the presumptive Enterobacteriaceae below detectable levels. The bacterial diversity of control and treated puree was studied by Illumina paired-end sequencing, using DNA extracted from the puree samples incubated at 25 °C for 24 h. The controls and the puree inoculated with the UJA2219 strain showed an almost-identical bacterial diversity profile, with Pseudomonadota (mainly Fam. Pseudomonadaceae -gen. Pseudomonas- and Enterobacteriace as the most abundant groups). The greatest differences in bacterial diversity were obtained in the puree treated with RP extract, showing a decrease in the relative abundance of Pseudomonadota (especially gen. Pseudomonas) and an increase of Bacillota (mainly of the genera Bacillus, Enterococcus and Lactococcus). Results from the study suggest that the antimicrobial preparations from strain UJA2219 have a potential for application in food biopreservation.
Notational Analysis on Goal Scoring and Comparison in Two of the Most Important Soccer Leagues: Spanish La Liga and English Premier League
Authors: Gouveia Vítor , Duarte João Pedro , Nóbrega António , Sarmento Hugo , Pimenta Eduardo , Domingos Flávio , Antunes Raul , Monteiro Diogo , Matos Rui , Amaro Nuno , Araújo Inês
Published: June 2023

Goals scored are the most important performance indicator and may be a good tool to analyze game style predominance within different leagues. The aims of this study are to describe how goals are scored in the two major soccer leagues in the season of 2020/21, and to compare both leagues to watch possible differences in the offensive sequences that lead to goal-scoring. The aims of this study were: to evaluate goal scoring trends among two main soccer leagues and to compare the two leagues. A total of 1409 goals were observed using notational analysis. The total results show a dominance of positional play/fast attack (n = 38%), followed by set pieces as the second means to score (n = 32%) with corner kicks having a great impact (n = 36%) within set pieces goals. The most goals were scored by short possessions (n = 50%) with short passes number (n = 57%), with the recovery of the ball in the offensive third (n = 47%) and the huge dominance of finalization in central areas inside the box (n = 78%). Spanish La Liga scored more by positional play/fast attack, with most of the possessions starting in the medium third and using more the “central wing goal area” and “between wings left in the box” end zones when compared with English Premier League. The latter has more goals from counterattacks and corner kicks with very long possessions. Most of the possessions start in the offensive third and more goals are scored in “central wing between penalty and goal area” in comparison with Spanish La Liga. Coaches must be aware of the trends of the game and of the differences across leagues to prepare themselves when working in different countries to each specific characteristic.
A Review on Food Safety: The Case of Citrobacter sp., Fish and Fish Products
Authors: Cortés-Sánchez Alejandro De Jesús , Salgado-Cruz María de la Paz , Diaz-Ramírez Mayra , Torres-Ochoa Erika , Espinosa-Chaurand Luis Daniel
Published: June 2023

Fish is considered a highly nutritious food that constitutes the human diet, produced through fishing and aquaculture activities, to be marketed and consumed around the world in different presentations and culinary preparations. Fish is also very susceptible to spoilage and contamination by microorganisms throughout the food chain, which may be part of the usual microbiota or incorporated into food derived from inadequate hygiene practices in the food industry. Fish has been associated worldwide with disease outbreaks derived from consumption, where various bacteria and/or metabolites (biogenic amines) are some of the main casual agents. Citrobacter sp. is considered a pathogen in fish, as well as in humans, derived from the consumption of contaminated food, generating infections or histamine poisoning as it is part of the generating microbiota. Therefore, the objective of this document is to provide information on Citrobacter sp. through a general perspective on animal health and the safety of fish and fish products. Diseases derived from Citrobacter sp. contamination are included, as well as control, prevention, and sanitary legislation actions to promote animal health and the safety of foods of aquatic origin and the protection of public health.
An Efficient Algorithm and Architecture for the VLSI Implementation of Integer DCT That Allows an Efficient Incorporation of the Hardware Security with a Low Overhead
Authors: Chiper Doru Florin , Cracan Arcadie
Published: June 2023

In this paper, we propose a new hardware algorithm for an integer based discrete cosine transform (IntDCT) that was designed to allow an efficient VLSI implementation of the discrete cosine transform using the systolic array architectural paradigm. The proposed algorithm demonstrates multiple benefits specific to integer transforms with efficient hardware implementation and sufficient precision in approximating irrational transform coefficients for practical applications. The proposed integer DCT algorithm can be efficiently restructured into five regular and modular computational structures of lengths of four and one of length two called pseudo-cycle convolutions which translate into efficient VLSI implementations using systolic arrays. Moreover, besides an efficient VLSI implementation with high-speed performances due to the parallel decomposition of the proposed integer DCT algorithm, the proposed VLSI architecture uses a tag control mechanism that facilitates the integration of an obfuscation technique that significantly improves the hardware security with low overheads, maintaining all the implementation performances.
Using Machine Learning to Predict Wind Flow in Urban Areas
Authors: BenMoshe Nir , Fattal Eyal , Leitl Bernd , Arav Yehuda
Published: June 2023

Solving the hydrodynamical equations in urban canopies often requires substantial computational resources. This is especially the case when tackling urban wind comfort issues. In this article, a novel and efficient technique for predicting wind velocity is discussed. Reynolds-averaged Navier–Stokes (RANS) simulations of the Michaelstadt wind tunnel experiment and the Tel Aviv center are used to supervise a machine learning function. Using the machine learning function it is possible to observe wind flow patterns in the form of eddies and spirals emerging from street canyons. The flow patterns observed in urban canopies tend to be predominantly localized, as the machine learning algorithms utilized for flow prediction are based on local morphological features.
Research on Repressing Allergen Cry j 1 Released from Japanese Cedar Pollen Using Todomatsu Oil
Authors: Lin Yichun , Xiao Kai , Wang Qingyue , Lu Senlin , Wang Weiqian , Seguchi Akifumi
Published: June 2023

Japanese cedar (JC, Cryptomeria japonica) pollen allergens are the primary cause of JC pollinosis, a widespread seasonal allergic disorder and a significant public health issue in Japan. Nevertheless, rare information on repressing the pollen allergens released from JC pollen is available. This study aims to validate the repression of allergen Cry j 1 (the dominant JC pollen allergen that triggers JC pollinosis) using todomatsu oil produced from Abies sachalinensis waste, through surface plasmon resonance (SPR) experiments, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and blind docking simulation. The findings revealed that todomatsu oil reduces the antibody reactivity and content of allergen Cry j 1 by 85.96% and 42.12%, respectively. The docking modeling suggested that alkyl hydrophobic forces, pi–sigma bonding, and hydrogen bonding are the principal motivating forces for todomatsu oil components to dock with allergen Cry j 1. More than 50% of the amino-acid residues docked by each todomatsu oil component (except α-pinene) are hydrophobic. Furthermore, oil components, especially β-maaliene, exhibited potent repression of allergen Cry j 1. Consequently, todomatsu oil, due to its inexpensive, available, convenient, and ecologically beneficial nature, is a viable measure to repress allergen Cry j 1.
Comparative Evaluation of Rainfall Forecasts during the Summer of 2020 over Central East China
Authors: Guo Yakai , Shao Changliang , Su Aifang
Published: June 2023

By using various skill scores and spatial characteristics of spatial verification methods and traditional techniques of the model evaluation tool, the gridded precipitation observation, known as Climate Prediction Center Morphing Technique, gauge observation and three datasets that were derived from local, Shanghai, and Grapes models, respectively, were conducted to assess the 3 lead day rainfall forecast with 0.5 day intervals during the summer of 2020 over Central East China. Results have shown that the local model generally outperforms the other two for the most skill scores but usually with relatively larger uncertainties than the Shanghai model, and it has the least displacement errors for moderate rainfall among the three datasets. However, the rainfall of the Grapes model has been heavily underestimated and is accompanied with a large displacement error. Both the local and Shanghai model can effectively forecast the large-scale convection and rainstorms but over forecast the local convection, while the local model likely over forecasts the local rainstorms. In addition, the Shanghai model slightly favors over forecasting on a broad scale range and a broad threshold range, and the local model slightly misses the rainfall exceeding 100 mm. Generally, for a broadly comparative evaluation on rainfall, the popular dichotomous methods should be recommended when considering reasonable classification of thresholds if the accuracy is highly demanding. In addition, most spatial methods are suggested to conduct with proper pre-handling of non-rainfall event cases. Especially, the verification metrics including spatial characteristic difference information should be recommended to emphasize rewarding the severe events forecast under a global warming background.
Using a Bone Conduction Hearing Device as a Tactile Aid
Authors: Kompis Martin , Langmair Manfred , Mantokoudis Georgios , Weder Stefan , Gawliczek Tom , Caversaccio Marco Domenico
Published: June 2023

Background: With the advent of cochlear implants, tactile aids for the profoundly deaf became obsolete decades ago. Nevertheless, they might still be useful in rare cases. We report the case of a 25-year-old woman with Bosley–Salih–Alorainy Syndrome and bilateral cochlear aplasia. Methods: After it was determined that cochlear or brainstem implants were not an option and tactile aids were not available anymore, a bone conduction device (BCD) on a softband was tried as a tactile aid. The usual retroauricular position and a second position close to the wrist, preferred by the patient, were compared. Sound detection thresholds were measured with and without the aid. Additionally, three bilaterally deaf adult cochlear implant users were tested under the same conditions. Results: At 250–1000 Hz, sounds were perceived as vibrations above approximately 45–60 dB with the device at the wrist. Thresholds were approximately 10 dB poorer when placed retroauricularly. Differentiation between different sounds seemed difficult. Nevertheless, the patient uses the device and can perceive loud sounds. Conclusions: Cases where the use of tactile aids may make sense are probably very rare. The use of BCD, placed, e.g., at the wrist, may be useful, but sound perception is limited to low frequencies and relatively loud levels.
Twi Machine Translation
Authors: Gyasi Frederick , Schlippe Tim
Published: June 2023

French is a strategically and economically important language in the regions where the African language Twi is spoken. However, only a very small proportion of Twi speakers in Ghana speak French. The development of a Twi–French parallel corpus and corresponding machine translation applications would provide various advantages, including stimulating trade and job creation, supporting the Ghanaian diaspora in French-speaking nations, assisting French-speaking tourists and immigrants seeking medical care in Ghana, and facilitating numerous downstream natural language processing tasks. Since there are hardly any machine translation systems or parallel corpora between Twi and French that cover a modern and versatile vocabulary, our goal was to extend a modern Twi–English corpus with French and develop machine translation systems between Twi and French: Consequently, in this paper, we present our Twi–French corpus of 10,708 parallel sentences. Furthermore, we describe our machine translation experiments with this corpus. We investigated direct machine translation and cascading systems that use English as a pivot language. Our best Twi–French system is a direct state-of-the-art transformer-based machine translation system that achieves a BLEU score of 0.76. Our best French–Twi system, which is a cascading system that uses English as a pivot language, results in a BLEU score of 0.81. Both systems are fine tuned with our corpus, and our French–Twi system even slightly outperforms Google Translate on our test set by 7% relative.
Deep-Learning-Based Segmentation of Extraocular Muscles from Magnetic Resonance Images
Authors: Qureshi Amad , Lim Seongjin , Suh Soh Youn , Mutawak Bassam , Chitnis Parag V. , Demer Joseph L. , Wei Qi
Published: June 2023

In this study, we investigated the performance of four deep learning frameworks of U-Net, U-NeXt, DeepLabV3+, and ConResNet in multi-class pixel-based segmentation of the extraocular muscles (EOMs) from coronal MRI. Performances of the four models were evaluated and compared with the standard F-measure-based metrics of intersection over union (IoU) and Dice, where the U-Net achieved the highest overall IoU and Dice scores of 0.77 and 0.85, respectively. Centroid distance offset between identified and ground truth EOM centroids was measured where U-Net and DeepLabV3+ achieved low offsets (p > 0.05) of 0.33 mm and 0.35 mm, respectively. Our results also demonstrated that segmentation accuracy varies in spatially different image planes. This study systematically compared factors that impact the variability of segmentation and morphometric accuracy of the deep learning models when applied to segmenting EOMs from MRI.
Oxalate Homeostasis in Non-Stone-Forming Chronic Kidney Disease: A Review of Key Findings and Perspectives
Authors: Stepanova Natalia
Published: June 2023

Chronic kidney disease (CKD) is a significant global public health concern associated with high morbidity and mortality rates. The maintenance of oxalate homeostasis plays a critical role in preserving kidney health, particularly in the context of CKD. Although the relationship between oxalate and kidney stone formation has been extensively investigated, our understanding of oxalate homeostasis in non-stone-forming CKD remains limited. This review aims to present an updated analysis of the existing literature, focusing on the intricate mechanisms involved in oxalate homeostasis in patients with CKD. Furthermore, it explores the key factors that influence oxalate accumulation and discusses the potential role of oxalate in CKD progression and prognosis. The review also emphasizes the significance of the gut–kidney axis in CKD oxalate homeostasis and provides an overview of current therapeutic strategies, as well as potential future approaches. By consolidating important findings and perspectives, this review offers a comprehensive understanding of the present knowledge in this field and identifies promising avenues for further research.