bluuebunny/crossref_metadata_2025 huggingface dataset:
Green synthesis of copper nanoparticles using Artemisia annua aqueous extract and its characterization, antioxidant and burn wound healing activities
Authors: Karimi Behnaz , Mardani Masoumeh , Kaboutari Jahangir , Javdani Moosa , Albadi Jalal , Shirian Sadegh
Published: March 2023

the current study reports a green method for synthesizing copper nanoparticles (CuNPs) using aqueous extract of Artemisia annua in a simple, and eco-friendly manner. The green synthesized CuNPs were characterized using UV–Vis, FT-IR spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM) and Energy Dispersive X-ray analysis (EDX). The UV-Vis spectroscopy showed maximum absorption at 250 nm, and the average diameter of the synthesized NPs was found to be 35 nm. FTIR showed the presence of functional groups in bioactive constituents responsible for reducing Cu+ ions. The antioxidant activity of the synthesized NPs was evaluated by DPPH assay, and at 100 µg/ml green synthesized CuNPs, the highest antioxidant activity was obtained, which was 67 % and an IC50 value of 73.48 µg/ml. In the in vivo experiment, after inducing 2nd degree burn wounds, the rats were randomly divided into five group including the untreated control, treatment with eucerin basal ointment, treatment with 0.2 % CuNP ointment, treatment with 0.2 % CuNP-loaded ointment, and treatment with 5 % Artemisia annua extract twice a day for 21 days. Application of biosynthesized CuNPs ointment significantly improved the healing percentage and hydroxyproline content compared to the control. Additionally, histopathology studies showed significant progress in re-epithelization, collagen fiber deposition, and angiogenesis in the biosynthesized CuNPs in comparison to the control. The greenly synthesized CuNPs had enhanced antioxidant, and wound healing properties and, therefore, could be considered as a promising candidate in the biomedical field.
Ground strains induced by the 2022 Hunga-Tonga volcanic eruption, observed by a 1500-m laser strainmeter in Kamioka, Japan
Authors: Takamori Akiteru , Araya Akito , Miyo Kouseki , Washimi Tatsuki , Yokozawa Takaaki , Hayakawa Hideaki , Ohashi Masatake
Published: March 2023

In this study, we detected the horizontal ground strains, induced by the atmospheric Lamb wave emitted from the 2022 eruption of the Tong–Hunga Ha’apai undersea volcano, at an underground observatory in Kamioka, Japan. The observed strains were in the range of 10-11 to 10-10 and precisely measured using a 1500-m laser strainmeter with a high resolution of the order of 10-12. The strainmeter was constructed in a tunnel of KAGRA gravitational-wave telescope. Our observations showed that strain and atmospheric pressure were clearly correlated, resulting in a regression coefficient of -(2.3 –3.7) × 10-10 strain/hPa. This finding was compared with the responses under regular pressure conditions and the estimations by the local deformation model and the traveling wave model. The observed coefficients for the Lamb wave had smaller magnitudes than those under regular conditions and has a value between that of the two theoretical models. These results reflect the intermediate scale of pressure distribution of the Lamb wave between the assumptions of the simple models. The strain variations were also found to have started earlier than the corresponding pressure changes at the observation site with a characteristic time shifts ranging in 25–155 seconds. This finding indicates that the remote pressure loading caused the ground strains, and such interpretation was enabled by the specific pressure distribution of the Lamb wave.
Interactions between self-reported pain intensity and affects in daily life: a pilot study comparing individuals with chronic pain with primary and secondary pain disorders
Authors: Ledermann K. , Piccolo Mayron , Recabarren R. , Blanc J. , Martin-Soelch Ch. , Sprott H.
Published: March 2023

Background and objectives:
Chronic pain is frequently associated with depression and anxiety disorders. A relationship between affects and pain intensity in everyday life has been previously documented using Ecological momentary assessment technique (EMA) with a particular focus on the exacerbating effect of stress and depressive symptomatology on pain intensity in daily life of specific samples of chronic pain patients. With the introduction of the new category of chronic primary pain in the ICD-11, which is strongly characterized by emotional distress, it is interesting to compare primary and secondary chronic pain patients with regard to the relationships between pain intensity and affects in everyday life.
Methods:
Here, 16 individuals with chronic pain (primary pain, N=10; secondary pain, N=6) completed a pain diary during 2 consecutive weeks, 3 times a day, within fixed time periods (morning, midday, evening). Self-report questionnaires were used to measure depressive symptomatology and anxiety. Momentary affects were grouped as positive and negative affects. Data analyses were performed using mixed model and Pearson’s correlation analyses.
Results:
Independently of group, individuals reporting more positive affect reported significantly lower pain intensity. Individuals in the primary pain group tended to report higher pain intensity compared to the secondary pain group. Exploratory analyses indicated significant correlations of pain intensity and depressive symptoms, and of pain intensity and affects only in the group with primary pain.
Conclusions:
Despite their preliminary nature due to the small sample size, our findings indicate a general beneficial effect of positive momentary affects on pain intensity in daily life. They also provide first evidence for differences in the perception of pain intensity between primary and secondary chronic pain patients with regard to the relationship between pain intensity and affects.
Commensal adaptation of yeast Saccharomyces cerevisiae to grape-skin environment
Authors: Watanabe Daisuke , Hashimoto Wataru
Published: March 2023

Saccharomyces cerevisiae, an essential player in alcoholic fermentation during winemaking, is rarely found in intact grapes. Although grape-skin environment is unsuitable for S. cerevisiae’s stable residence, S. cerevisiae or its relatives can increase population on grape berries after colonization during raisin production. Here, we addressed commensal adaptation of S. cerevisiae to grape-skin ecosystem. The yeast-like fungus Aureobasidium pullulans, a major grape-skin resident, exhibited broad spectrum assimilation of plant-derived carbon sources, including ω-hydroxy fatty acid, arising from degradation of plant cuticles. In fact, A. pullulans encoded and expressed esterase for cuticle degradation. When intact grape berries were used as a sole carbon source, non-fermentative A. pullulans or other residents accelerated S. cerevisiae’s alcoholic fermentation via increasing carbon availability. Thus, degradation and utilization of grape-skin materials by resident microbiota may account for their indigenous and S. cerevisiae’s commensal behaviors. Such plant-microbe symbiotic interaction may be a prerequisite for triggering spontaneous food fermentation.
HuR/miR-124-3p/VDR complex bridges lipid metabolism and tumor development in colorectal cancer
Authors: Huang Fengxing , Bu Luping , Li Mengting , Wang Youwei , Zhang Runan , Shao Yu , Lin Kun , Yang Hong , Zhao Qiu , Liu Lan
Published: March 2023

Background:Maintaining lipid homeostasis to prevent lipotoxicity is crucial for various tumors, including colorectal cancer (CRC). Hu-antigen R (HuR) is a member of the RNA binding protein family and overexpressed in many cancer types, which implicted that in regulating cell proleferation, migration, invasion, and lipid metabolism. However, the role of HuR in regulating abnormal lipid metabolism of CRC is unknown.Methods:Western blot was performed to screen differentially expressed HuR between CRC tissues and adjacent normal tissues. Lipidomic profiling, RNA sequencing (RNA-seq), Cell Counting Kit-8 (CCK-8), total cholesterol and triglycerides assays testified the critical role of HuR/miR-124-3p/VDR complex in CRC cells. RNA pull-down and luciferase reporter Assays were performed to verify the interaction between HuR protein and the VDR mRNA. We also conducted a mouse xenograft model to elucidate the effect of HuR on lipid homeostasis and proliferation in vivo.Results:Our study identified that HuR promotes the expression of VDR, then modulates lipid homeostasis by enhancing TG and TC levels in CRC. Here, our study demonstrated that overexpressing HuR enhanced the expression of VDR through directly binding to its CDS and 3’-UTR. Simultaneously, HuR also indirectly affecting VDR by inhibiting miR-124-3p. We identified that HuR can suppress the expression of miR-124-3p, while miR-124-3p can bind to 3’-UTR of VDR to inhibit the expression of VDR. Moreover, xenograft models showed that targeting HuR suppressed the expression of VDR, blocked TG and TC formation, then suppressed CRC growth.Conclusion:Our findings propose a regulatory connection between HuR, miR-124-3p and VDR in CRC cells. We suggested that HuR/miR-124-3p/VDR complex modulates lipid homeostasis by influencing TG and TC formation in CRC, and may provide a potential target for CRC treatment and prevention.
Development of the WHO Eye care competency framework
Authors: Yu Mitasha , Keel Stuart , Mariotti Silvio , Mills Jody-Anne , Müller Andreas
Published: March 2023

Background
The eye care workforce, particularly in lower resource settings, face challenges of limited integration into the health system, limited workforce capacity, mismatch of workforce to population need and poor quality of care. In recognition of these challenges, coupled with a gap in existing tools, provides a strong rationale for the development of the Eye care competency framework (ECCF).
Methods
A mixed methods approach was utilised to develop and validate the ECCF. Content was developed by extracting relevant components of existing frameworks used both within and outside of eye care. A diverse technical working group provided feedback and guidance on the structure, design, and content to create a preliminary draft. Competencies and activities were validated using a modified-Delphi study, and the framework was then piloted at four sites to understand how the tool can be implemented in different settings.
Results
The final version of the ECCF included eight outcomes, nine guiding principles, and content of each of the key elements, including the six domains, 22 competencies, 21 activities, 193 behaviours and 234 tasks, and the knowledge and skills that underpin them. 95/112 participants from the six WHO regions completed the modified-Delphi study, yielding an average of 96% agreement across the competencies and activities in the ECCF. The pilot showcased the versatility and flexibility of the ECCF, where each of the four sites had a different experience in implementing the ECCF. All sites found that the ECCF enabled them to identify gaps within their current workforce documentation.
Conclusions
The ECCF was developed using a collaborative approach, reflecting the opinions of participants and stakeholders from all around the world. The comprehensive competencies and activities developed in the ECCF encompass the diverse roles of eye care workers, and thus encourage multi-disciplinary care and better integration into the health system. It is recommended that eye care workforce planners and developers use the ECCF to support workforce development and focus on the quality and scope of eye care service provision.
Impact of feed, light and access to manipulating material on tail biting in pigs with intact tails
Authors: Wallgren Per , Johansson Magnus , Wallgren Torun , Susic Zeljko , Sigfridson Kerstin , Johansson Sven-Erik
Published: March 2023

Background
Tail biting is a welfare issue that also has economic consequences due to infections and illthrift. This study aimed to reduce tail injuries in a high-performing non-tail-docking pig herd with increasing incidences of tail injuries.
Results
During eleven years preceding the trial, the annual incidence of tail injuries registered at slaughter had increased from 3% to 10%. It was positively correlated to a high weight gain and negatively correlated to day length. The overall incidence of tail injuries during the four years preceding the trial was 9.2% with significant differences between four identical buildings for fatteners (I<II<III<IV). During the adaption period of 6 months and trial period of 12 months, all pigs consumed a feed with increased levels of amino acids, trace elements and fibres, and the four buildings achieved different illumination strategies: I standard fluorescent tubes with an invisible flickering of 30-40% for 14 hours per day; II non-flickering led light for 14 hours per day; III standard fluorescent tubes for 2 hours per day (control); IV similar as III, but with free access to hay silage. During the adaption period, the incidence of tail injuries decreased significantly in all buildings and corresponded to 5.4%. The largest decrease (from 11.4 to 4.3%) was obtained in IV with free access to hay silage. During the trial, the mean incidence of tail injuries decreased to 3.0%. There were no differences in treatment incidences of individual pigs due to tail biting between buildings, but the use of enriched pellets due to anxiety in pens was lowest in II. The incidence of tail injuries in I was significantly higher than in II and III.
Conclusions
The incidence of tail biting in fast growing non-tail-docked pigs was successfully reduced by supplementing the feed with amino acids, trace elements, vitamins and fibres. Additional manipulative material accelerated that process and non-flickering illumination may have an impact in preventing tail biting. The results obtained argue for the un-necessity of tail-docking pigs, provided that the needs of the pigs in terms of feed ingredients, stocking density and access to manipulative materials are fulfilled.
Impact of the COVID-19 Pandemic Crisis on Turnover Intention Among Nurses in Emergency Departments in Thailand: A Cross Sectional Study
Authors: Sungbun Songwut , Naknoi Siripan , Somboon Panjasilpa
Published: March 2023

Background: During the COVID-19 pandemic in Thailand, a large volume of COVID-19 patients was referred to hospital emergency departments (EDs). This increased job demand and job strain among ED nurses, resulting in a high risk for intention to leave their organization. This study had two aims: 1) to investigate turnover intention during the COVID-19 pandemic among ED nurses; and 2) examine the effect of organization resources, maladaptive regulation, and job burnout on nurses’ turnover intention.
Method: This cross sectional study investigated 322 ED nurses. We divided participants into two groups: dark-red zone areas (pandemic crisis areas) and non-red zone areas (non-pandemic crisis areas). Descriptive statistics, Pearson’s correlation analysis, and multiple linear regression (stepwise method) were used to analyze factors that predicted turnover intention.
Results: Most participants were female and the mean age was 34.54 years. Overall, the mean score for the Turnover Intention Scale was 18.27±4.69 and 72.8% of ED nurses in dark-red zone areas desired to leave their organization. During the pandemic, motivation, exhaustion, and cognitive impairment positively influenced turnover intention among ED nurses in dark-red zone areas. Low availability of organization resources was associated with an increase in the turnover intention rate. Maladaptive regulation, exhaustion, and cognitive impairment positively influenced turnover intention among ED nurses in non-red zone areas.
Conclusion: Exhaustion and cognitive impairment are important dimensions of burnout that are associated with turnover intention among ED nurses. We suggest that promoting safe workplace environments and implementing interventions to reduce job demand and job strain for ED nurses may reduce turnover intention.
Salmonella Durban meningitis : case report and genomics study
Authors: Diasi Christelle Nanga , Ceyssens Pieter-Jan , Vodolazkaia Alexandra , Mukovnikova Marina , Dorval Sarah , Mattheus Wesley , Bauraind Olivia
Published: March 2023

Background

Bacterial meningitis caused by non-typhoid Salmonella can be a fatal condition which is more common in developing countries than in developed ones.

Case presentation

We report the case of a Salmonella meningitis in a Belgian six-month old male infant. The first clinical examination was reassuring but after a few hours his general state was deteriorating which led to a blood test and a lumbar puncture. The cerebrospinal fluid analysis was compatible with a bacterial meningitis which was later identified by the NRC (National Reference Center) as Salmonella enterica serovar Durban.

Conclusions

This serovar of Salmonella is extremely uncommon. An extended genomic analysis showed relatedness to historical cases with a connection to Guinea. In this paper, we describe the clinical presentation, the genomic typing and probable sources of infection.
Determinants of COVID-19 vaccine uptake among Nigerians: evidence from a cross- sectional national survey
Authors: OJO Temitope Olumuyiwa , OJO Ayodeji Oluwole , OJO Olajumoke Elizabeth , AKINWALERE Bosede Olufunmilayo , AKINWUMI Adebowale Femi
Published: March 2023

Objectives. This study assessed the factors influencing COVID-19 vaccine uptake among Nigerian households. Data from the COVID-19 High-Frequency Phone Survey of Households that was collected in late 2021-2022 by the National Bureau of Statistics was used for this study. Relevant data were analyzed using descriptive statistical tools and the multivariate binary logistic regression model.
Results: Out of 2370 respondents, only 32.8 percent of the respondents were vaccinated against COVID-19. Respondents living in urban areas (34.4%) had a higher level of COVID-19 vaccine uptake relative to those living in rural Nigeria (30.9%). Results from the Multivariate Regression model revealed that older adults, public servants, and those who obtained vaccine information from health workers, government, and the mass media were more likely to be vaccinated. The study also found respondents living in North Central, North East, South West and South South regions had higher odds of being vaccinated.
Conclusions: The study recommends increased media campaigns and advocacy for COVID-19 vaccination in the South East and North West regions. Younger persons should be targeted with COVID-19 information given that those aged 18-29 years were less likely to be vaccinated.
Composition and evolution of the gut microbiota of growing puppies is impacted by their birth weight
Authors: Garrigues Quentin , Apper Emmanuelle , Rodiles Ana , Rovere Nicoletta , Chastant Sylvie , Mila Hanna
Published: March 2023

Low birth weight puppies present an increased risk of neonatal mortality, morbidity, and some long-term health issues. Yet it has not been investigated if those alterations could be linked to the gut microbiota composition and evolution. 57 puppies were weighed at birth and rectal swabs were performed at 7 time points from birth to 56 days of age. Puppies were grouped into four birth weight quartiles, with individuals from the first quartile (Q1) presenting the lowest birth weight and the fourth quartile (Q4) the highest. 16S rRNA gene sequencing was used to highlight differences in the fecal microbiota. During the first three weeks, the relative abundance of facultative anaerobic bacteria such as E. coli, C. perfringens and Tyzzerella was higher in Q1 feces, but they catch back with the other groups afterwards. Q4 puppies showed higher abundances of Faecalibacterium and Bacteroides during the neonatal period, suggesting an earlier maturation of their microbiota. The results of this study suggest that birth weight impact the initial establishment of the gut microbiota in puppies. Mainly, a lower initial oxygen level in the gut of Q1 puppies may cause the proliferation of opportunistic bacteria. Innovative strategies would be desired to deal with altered gut microbiota in low birth weight puppies aming to improve their survival and long term health.
Race and Income Inequality: A Case of the U.S.A
Authors: Fawaz Fadi , Bradds Jackson , Taylor Rima
Published: March 2023

Racial inequality in the U.S. is typically described in terms of categorical discrimination. We use released data from the U.S. from 1973- 2019 to show income inequality by perceived skin color and racial self-identification and examine which measure better captures racial disparities. We document color and racial hierarchies across the Americas, revealing some unexpected patterns. Whereas blacks at times have higher mean incomes than other racial populations. Results complicate theoretical debates about U.S. racial exceptionalism and methodological debates about how best to measure income. Rather than supporting one measure over another, our analysis underscores race’s multidimensionality.
We employ a fixed effects regression model to uncover structural change in the Kuznets curves for the U.S. aggregate, Black, White, Asian, and Hispanic American segments of the population. Unlike to prior studies, we find that the Kuznets curves does not switch to a U-shape. We found conclusive evidence linking income inequality to race.
JEL Codes: C13, C30, E25, O51
Sustainability Performance of Rural Municipalities in Germany
Authors: Karami Mahdi , Madlener Reinhard
Published: March 2023

This study assesses the sustainability performance of rural municipalities, proposing a novel sustainability benchmarking system. For this purpose, the performance of selected rural municipalities in Germany along the dimensions energy & environment (E), quality of life (Q), local economy (L),and digitalization (D) is examined based on a predefined set of indicators. This benchmarking tool can assist local communities and provide them with insights on how to improve their sustainability performance. We find that the data available to explain the performance of rural communities in Germany is relatively scarce and unevenly spread. Most databases and sustainable development reportsare updated only every couple of years and often do not report the performance of rural municipalities but only larger (more urban) ones. Our study contributes to the sustainability literature by examining the most general sustainability policies, indicators, and rating systems at the rural level. Adding digitalization as a technological value makes our approach more comprehensive than many others, and accounts for the important new dimension of societal transition. The recommendations provided can be of interest for municipalities seeking to either learn from each other or have some systematic, repeatable self-assessment and thus sustainability guidance over time.
Genome-wide identification of the auxin transporter gene families in sweet potato (Ipomoea batatas) and their expression during tuberization
Authors: Mathura Sarah R. , Sutton Fedora , Bowrin Valerie
Published: March 2023

Auxin is a plant phytohormone that is essential for the initiation of tuberization in sweet potato (Ipomoea batatas (L.) Lam.). Auxin exerts these effects via polar auxin transport facilitated by various auxin influx and efflux carriers. It is unclear which members of the auxin transporter families: PIN, PILS, AUX/LAX, and ABCB, are involved in sweet potato tuber initiation and development. Therefore, a genome-wide analysis of the I. batatas auxin transporter genes was conducted, and their expression patterns during storage root initiation and development were analyzed. Five IbLAX, 16 IbPIN, 12 IbPILS, and 34 IbABCB family members were identified. These genes showed high conservation among families based on their intron-exon structure, motif composition, and phylogenetic analysis. Additionally, the promoter regions of these genes had various cis-acting regulatory elements involved in hormone, light, and developmental responses. The auxin transporter genes were expressed in various sweet potato tissues, and many were differentially expressed during storage root development. IbLAX1, IbPIN13, IbPILS7, IbABCB1, and IbABCB14 showed up-regulated expression during tuber initiation. This study characterizes these auxin transporter gene families for the first time. These results are an important reference for validation studies to determine the specific functions of these genes and their auxin transporting capability.
The saclayvirus Aci01-1 very long and complex fiber and its receptor at the Acinetobacter baumannii surface
Authors: Pourcel Christine , Ouldali Malika , Tavares Paulo , Essoh Christiane
Published: March 2023

The Acinetobacter baumannii bacteriophage Aci01-1 that belongs to the genus Saclayvirus of the Caudoviricetes order has an icosahedral head and a contractile rigid tail. We report that Aci01-1 has, attached to the tail conical tip, a remarkable 146 nm-long flexible fiber with seven beads and a terminal knot. Its putative gene coding for a 241.36 kDa tail fiber protein is homologous to genes in Aci01-1-related and unrelated phages. We also identified a putative receptor of the phage on the bacteria capsule that is hypothesized to interact with the Aci01-1 long fiber.
Construction of immune score and its prognostic value in invasive lobular carcinoma of the breast using computational pathology analysis
Authors: Wang Liye , Sun Peng , Xu Fei , Zheng Qiufan , Jiang Kuikui , Hong Ruoxi , Wang Shusen
Published: March 2023

Background
Previous studies have shown that high level of TILs in invasive lobular carcinoma (ILC) is associated with poor prognosis, contrary to that in TNBC and HER2-positive breast cancer. We aimed to systematically explore the expression of various tumor-infiltrating immune cell markers and immune checkpoints in ILC and their relationship with prognosis.
Methods
The expression of immune cell markers CD4, CD8, CD20, CD56, CD68, FOXP3 and immune checkpoints PD-1, PD-L1 and CTLA-4 in the microenvironment were detected by immunohistochemistry and their densities were quantified by computational pathology analysis. Then, the LASSO cox regression model was used to construct an immune score (IS) and further evaluate its prognostic value. We further explored the prognostic value of CD68 and FOXP3 with the transcriptome data of ILC patients in the METABRIC database.
Results
In our ILC cohort, the low density of CD4, CD8, CD20, CD56, CD68, FOXP3, PD-1 and PD-L1 had significantly longer disease-free survival (DFS) and overall survival (OS), however, the low density of CTLA-4 was associated with shorter DFS and OS. Based on this, each patient was given a binary score (0 for low, 1 for high) for each immune cell type (CD68 and FOXP3). Patients with low-IS had significantly prolonged DFS (HR 0.19, 95%CI 0.06-0.64, p < 0.0001) and OS (HR 0.14, 95%CI 0.03-0.56, p< 0.0001). Multivariate analysis revealed that IS was an independent prognostic indicator for DFS and OS. Further analysis showed that IS may increase the prognostic value of TNM stage. We further explored the prognostic role of CD68 and FOXP3 in the transcriptional level and the corresponding ISm in the METABRIC dataset, and found that low proportion of CD68 and FOXP3 and their ISm were associated with longer OS, and ISm was also an independent prognostic factor for OS. Furthermore, we revealed the possible mechanisms that the level of tumor-infiltrating immune cells in ILC influence prognosis.
Conclusions
We systemically evaluated the densities of six immune cells and three immune checkpoints and further constructed an IS model, which is a promising biomarker to distinguish the prognosis in ILC patients.
Does the Combination of Intrathecal Fentanyl and Morphine Improve Clinical Outcomes in Patients Undergoing Lumbar Fusions?
Authors: Villavicencio Alan , Taha Hash Brown , Burneikiene Sigita
Published: March 2023

Intrathecal morphine (ITM) has been widely effective in improving postoperative outcomes in patients undergoing a multitude of surgeries, including lumbar spine fusion. A major limitation of ITM administration is the increase in the incidence of respiratory depression in a dose-dependent manner. One way to bypass this is to use a more potent opioid with a shorter half-life, such as fentanyl. This is a retrospective analysis of patients who underwent one- or two-level transforaminal lumbar interbody fusions. The patients received one of two interventions: 0.2mg intrathecal duramorph/morphine (ITM group; n = 70), 0.2mg duramorph + 50 mcg fentanyl (ITM + fentanyl group; n = 68) and the control group (n = 102). Primary outcomes included postoperative pain (Visual Analog Scale) and opioid intake (MED – morphine equivalent dosage, mg) for postoperative days (POD) 1–4. Secondary outcomes included opioid-related side effects. One-way analyses of variance and follow-up post-hoc Tukey’s honest significant difference statistical tests were used to measure treatment effects. Significantly lower POD1 pain scores for both the ITM and ITM + fentanyl groups vs. control were detected, with no difference between the ITM vs. ITM + fentanyl groups. Similar results were found for POD1 MED intake. A multivariate regression analysis controlling for confounding variables did not attenuate the differences seen in POD1 pain scores while revealing that only the ITM + fentanyl predicted a decrease in POD1 MED intake. No differences were seen for postoperative opioid-related side effects. Our study provides support for supplementing a low dose of both intrathecal morphine and fentanyl to improve postoperative outcomes.
Novel SCN9A variant associated with congenital insensitivity to pain.
Authors: Yammine Tony , Aprahamian Raffi , Souaid Mirna , Salem Nabiha , Awwad Johnny , Farra Chantal
Published: March 2023

Background
Congenital insensitivity to pain (CIP) is a rare autosomal recessive syndrome characterized by lack of pain perception with a wide spectrum of clinical signs such as anosmia and hyposmia. SCN9A gene variants were found to be associated with CIP. We here report on a family with three CIP affected patients referred for genetic investigations.
Methods and Results
Whole exome sequencing analysis revealed the presence of a novel nonsense, homozygous SCN9A pathogenic variant: SCN9A (NM_001365536.1): c.4633G > T, p.(Glu1545*) in exon 26.
Conclusion
Our three Lebanese patients had CIP, urinary incontinence and normal olfactory function while two of them also presented with osteoporosis and osteoarthritis; this association of features has not been previously reported in the literature. We hope that this report would contribute to a better delineation of the spectrum caused by SCN9A pathogenic variants.
Potential effects of Electrotherapy on acquiring of malaria infection in Khartoum state
Authors: Mohamed¹ MusabAbduljalil , Elamin¹ Elamin Abdelkareem , Elbashier² Mohamed Mobarak , Mohamed Musab Abduljalil , Mohamed Hozifa Awadelkream Edriss
Published: March 2023

Objective: Electrotherapy is the electrical stimulation of tissues for therapeutic purposes the therapy uses specific wave length and frequency from the electromagnetic spectrum to produce desired physiological and chemical changes in the body. Insusceptibility to get malaria infection among patients treated with electrotherapy was locally observed, therefore the study was aimed to detect the potential effect of electrotherapy on acquiring of malaria infection in order to assess the management of the disease in Khartoum state.
Result: The study involved both sexes with 55males and 46 females, the percentage of males not being infected with malaria after electrotherapy sessions(84%) is more than females(67%) out of the total number, Interestingly one of the patients undergoing the electrotherapy sessions had diagnosed with severe malaria with mental disturbance, the symptoms of the severe malaria decreased from him and his condition improved after submitting to electrotherapy sessions. The exposing percentage of patients who did not acquire malaria after electrotherapy sessions was approximately 80% with P.value(0.203) compared to those infected with malaria after electrotherapy sessions. The study results suggests the potential hypothesis of existence of effect of electrotherapy on acquiring malaria infection, the study recommended to carry out further experimental in-vitro and in-vivo studies to prove and explain the mechanism of effect in order to assist in treatment and management of the disease.
SNORD11B-mediated 2′-O-methylation of primary let-7a in colorectal carcinogenesis
Authors: Pan Qiuhui , Bian Zhixuan , Xu Chang , Wang Xiaoying , Chen Yan , Mao Siwei , Wu Qi , Zhu Jiabei , Huang Nan , Zhang Yue , Ma Ji , Sun Fenyong
Published: March 2023

Evidence indicates that small nucleolar RNAs (snoRNAs) participate in tumorigenesis and development and could be promising biomarkers for colorectal cancer (CRC). Here, we examine the profile of snoRNAs in CRC and find that expression of SNORD11B is increased in CRC tumor tissues and cell lines, with a significant positive correlation between SNORD11B expression and that of its host gene NOP58. SNORD11B promotes CRC cell proliferation and invasion and inhibits apoptosis. Mechanistically, SNORD11B promotes the processing and maturation of 18S ribosomal RNA (rRNA) by mediating 2'-O-methylated (Nm) modification on the G509 site of 18S rRNA. Intriguingly, SNORD11B mediates Nm modification on the G225 site of MIRLET7A1HG (pri-let-7a) with canonical motif, resulting in degradation of pri-let-7a, inhibition of DGCR8 binding, reduction in mature tumor suppressor gene let-7a-5p expression, and upregulation of downstream oncogene translation. SNORD11B performs better than CEA and CA199 in diagnosing CRC. High expression of SNORD11B is significantly correlated with more advanced TNM stage and lymph node metastasis, which indicate poor prognosis.
Doped CeO 2 /Water Nanofluids Analyzed the performance of Thermal Conductivity and Heat Transfer: An Experimental and Theoretical Investigations
Authors: Pandey Abhishek Kumar , Tiwari Arun Kumar , Paliwal H. K.
Published: March 2023

A combined experimental and theoretical study on thermal conductivity, heat transfer specific heat, and electronic properties has been done for doped CeO2/water nanofluid. First, the sol-gel method was implemented for the synthesis of doped CeO2 nanoparticles and then a mixture of nanoparticles with different water concentrations (i.e. 0.5%,1%, 1.5%) in the form of nanofluid. X-ray diffraction and SEM analysis confirm the structural phase purity and homogeneous mixing of nanofluids. Experimental Thermal conductivity and specific heat of pure and 4f-doped CeO2 were estimated and found very close to our theoretical calculations. Experimental investigations have been carried out for the measurement of heat transfer using pure and doped CeO2/water nanofluid as the coolant. The experiments were aimed at determining the heat transfer and other thermal properties with different concentrations (0.5, 1.0, and 1.5 vol. %) and with various fluid flow rates (1.0 and 3.0 lpm). The heat transfer coefficient of nanofluids increases not only with an increase in the volume flow rate of the hot water but also increases with increase in the atomic number of dopant elements in CeO2. Electronic states show variation in band gap with doping which may also play an important role in the improvement of solar collectors. It is clear from experimental and theoretical findings that the thermal and electronic properties depend on number of valance electrons. Hence doping of 4f-element in CeO2 plays a vital role to increase the thermal conductivity and tunning of electronic properties leads to many applications in thermal sensors and solar cell-based industries.
On the Sequences of $(q,k)$-Generalized Fibonacci Numbers
Authors: Lelis Jean , Freitas Gersica , Kreutz Alessandra , Silva Elaine
Published: March 2023

In this paper, we consider the new family of recurrence sequences of (q,k)-generalized Fibonacci numbers. These sequences naturally extend the well-known sequences of k-generalized Fibonacci numbers and generalized k-order Pell numbers. We shall obtain a Binet-style formula and study the asymptotic behavior of dominant root of characteristic equation. Moreover, we shall prove some auxiliary results about these sequences. In particular, we characterize the first (q,k)-generalized Fibonacci numbers in terms of binary sequences.
Knowledge mapping and current trends of global research on snoRNA in the field of cancer
Authors: Xu Runsen , Hou Junhui , Wang Xia , Wang Yuan , Wang Kefeng
Published: March 2023

Backgroud:

Cancer is a major hazard to human health. Recently, small nucleolar RNA (snoRNA) has been found to be involved in the occurrence and development of cancer, which has potential diagnostic, prognostic and therapeutic value. The purpose of this study is to use the bibliometrics method to sort out and study the previous published papers.

Methods

We collected articles from the Web of Science Core Collection database in the field of snoRNA and cancer. Then, we used VOSviewer, Citespace, WPS and other software to visualize authors, Finally, we interpreted the data and analyzed the hotspots and frontiers of the research.

Results

The number of articles in this field was low in the early period, but exploded since 2008. According to the calculation of Prince's law, we believed that a stable cooperative group had been formed in this field. Chu, Liang and Montanaro, Lorenzo published the most papers, while Jiang, Feng were cited the most times. Three institutions published the most articles, namely Wuhan Univ, China Med Univ and Guangxi Med Univ. The journal with the most articles was Oncotarget. Through the analysis of countries/regions, it was found that the country with the most published articles was China. The analysis of keywords and burst words indicated that early studies mainly focused on the molecular mechanisms, but in recent years, it has gradually shifted to the direction of diagnosis, prognosis and therapy.

Conclusion

The research of snoRNA and cancer was a hot topic in recent years. Through analysis, we found that snoRNA was involved in the molecular mechanism of cancer development and can be used as a biomarker for clinical diagnosis and prognosis.
Three-Dimensional Femur Morphology Analysis for the Optimal Location of Subtrochanteric Osteotomy with an Implanted Wagner Cone Stem in Total Hip Arthroplasty for Crowe Type IV Developmental Dysplasia of the Hip
Authors: Tao Kun , Wang Shi-Cheng , Ma Xiao-Ying , Shao Long , Di Zheng-Lin , Huang Zhe-Yu
Published: March 2023

Background
This study aimed to accurately evaluate the matching of proximal and distal femoral segments and fitting of the femur–femoral stem in patients with Crowe type IV developmental dysplasia of the hip (DDH) who have undergone subtrochanteric osteotomy at different locations with an implanted Wagner cone stem to improve the rate of the bone union at the osteotomy site.
Methods
Three-dimensional femur morphology of 40 patients with Crowe type IV DDH was evaluated at each cross-section to determine the femoral cortical bone area. This study focused on five osteotomy lengths (2.5, 3, 3.5, 4, and 4.5 cm). The overlapped area between the proximal and distal cortical bone segments was defined as the contact area (S, mm2), and the contact area to distal cortical bone area ratio was defined as the coincidence rate (R). Three indicators were used to evaluate the matching and fitting of the osteotomy sites with the implanted Wagner cone stems: (i) higher S and R between the proximal and distal segments; (ii) the effective fixation length of the femoral stem at the distal segments being at least 1.5 cm; and (iii) osteotomy did not involve the isthmus.
Results
In all groups, S significantly decreased in the two proximal levels above the 0.5 cm level below the lesser trochanter (LT) compared with those below this level. In comparison, at osteotomy lengths from 2.5 to 4 cm, R significantly decreased in the three proximal levels. The optimal osteotomy levels ranged from 1.5 and 2.5 cm below the LT for an appropriately sized stem.
Conclusions
Subtrochanteric osteotomy at the optimal level not only ensures fitting of the femur–femoral stem but also meets the requirements of a higher S and R to ensure adequate reduction and immobilization at the osteotomy site, which may contribute to the bone union. Although the optimal osteotomy level varies with the size of the femoral stem and the length of the subtrochanteric osteotomy, the optimal osteotomy levels for an appropriately sized Wagner cone femoral stem implantation range from 1.5 to 2.5 cm below the LT.
Mixed neuroendocrine carcinoma consisting of small cell neuroendocrine carcinoma, metaplastic carcinoma, and invasive ductal carcinoma in the breast: A case report and review of the literature
Authors: Pan Zixin , Xiao Qinpei , Sun Kaixuan , Cui Jiayu , Zhang Baogang
Published: March 2023

Introduction: Neuroendocrine carcinoma accounts for less than 0.1% of all breast cancers and is a rare clinical cancer with neuroendocrine morphology, immunophenotype, and high-grade cytologic features. Case report: We report a case of a 54-year-old female patient with small cell neuroendocrine carcinoma of the right breast mixed with metaplastic carcinoma and invasive ductal carcinoma (non-specific type). Discussion: No cases of mixed occurrence of the three types of carcinoma in the breast have been reported in the literature. In this case, we made the diagnosis based on morphological features and immunohistochemistry, discussing the histogenetic mechanism and therapy prognosis. Conclusion: Due to the mixed occurrence of the three types of carcinoma, different morphological subtypes have different prognoses. Reporting the different components and their proportions becomes extremely important for clinical treatment and prognostic analysis in clinical work.
The influence of the lower order term and Hardy potential on existence and regularity results for nonlinear elliptic equations with degenerate coercivity
Authors: ACHHOUD F. , BOUAJAJA A. , MARAH A. , REDWANE H.
Published: March 2023

In this research we drive the existence and regularity results for solutions of some nonlinear degenerate Dirichlet problems containing two lower order terms, the first is a nonlinear convection term satisfying an optimal growth conditions and without any hypothesis of coercivity and the second is a zero order perturbation term, which called the hardy potential, that creates an obstruction to the existence of a solution. Not also that for right hand side, it is assumed that to be an Lm function with m ≥ 1.
MSC Classification: 35J60 , 35K05 , 35K67 , 35R09.
Dynamical Analysis of Two Cell Cycles with Delay Feedback Control
Authors: Ma Suqi
Published: March 2023

Double Hopf bifurcation of codimension 2 singularity in a type of two cells oscillator with interaction feedback connection is investigated. With the introduction of time delay which represents the communication time between cells, the stability switching phenomena is observed and double Hopf bifurcation occurs at the intersection point of Hopf lines. The geometrical scheme is applied in analyzing system stability underlying multiple time delays. The numerical simulation discovers that either the vicinity of bifurcating periodical solution on the margin of Hopf lines or the coexistence phenomena of five periodical solutions induced by stability switching phenomena. By applying the Schmidt dimensional reduction technique combined with the center manifold theory, the normal form of double Hopf point is calculated by parameter perturbation method and the near dynamics of double Hopf point is classified. The bifurcating periodical solutions and quasi-periodical solutions are observed which are in consistence with the numerical simulation results.
Daisy
Authors: Esslemont R. J. , Stephens A. J. , Ellis P.R.
Published: March 2023

This paper presents background information on the development of herd monitoring and a description of computerized record systems for the dairy herd developed by V.E.E.R.U., especially the 'DAISY' software package for small computer systems.
Cryptosporidiosis in Calves
Authors: Anderson Bruce C.
Published: March 2023

Cryptosporidium is a protozoan parasite of the suborder Eimeriorina which inhabits the microvillous border of intestinal enterocytes of calves, lambsand other animals and which has a life cycle pattern similar to that of other coccidia. Cryptosporidia have been recognized histologically in intestinal sections and by Giemsastained ileal and fecal smears, in field outbreaks of calf diarrhea. Recently, fecal flotation was used to detect oocysts in feces. They most often are in the presence of other enteric pathogens but some recent reports have attributed outbreaks solely to cryptosporidia
Diagnosis of Trichomoniasis
Authors: Mickelsen W. Duane
Published: March 2023

Trichomoniasis is a venereal disease of cattle characterized by infertility, early embryonic death and occasionally by pyometra and abortion. The etiological agent is a protozoan, Tritnchomonas fetus (T. fetus). Diagnosis of trichomonad pyometra by rectal palpation is a method not emphasized enough by most articles or text books. A skilled palpator will find this indeed a highly accurate method. Direct examination of the protozoan from an aborted fetus, or the pus from a pyometra, as well as culture in Diamonds media from preputial smegma of the bull or cervicovaginal mucus of infected cows is accurate in obtaining a diagnosis.Perhaps the disease shouldPerhaps the disease should be of reportable status because many infected cattle are sold through auction, or private treaty, only to spread the disease to other herds.
Feedlot Disease Losses
Authors: Griffin Dee
Published: March 2023

What does it really cost when an animal gets sick? A question that is extremely important to our organization.We feed over 250,000 animals a year in Southwest Kansas and the Oklahoma Panhandle. To support decision making we have a computer data management section which has helped us review and analyze the performance of selected groups of animals during the last three years.One question addressed when we started was the association of shrink to morbidity, mortality and cost of gain. Animal groups were selected for review based upon their homologous characteristics such as origin, weight, genetic background and season of the year fed.
Trichomoniasis
Authors: Ball L. , Mortimer R. G. , Cheney J.M. , Olson J. D.
Published: March 2023

Trichomoniasis is a veneral disease of cattle caused by Tritrichomonasfetus (T. Fetus). Its clinical signs are repeat breeding at both regular and irregular intervals and, occasionally, abortion. These are easy to observe in dairy cattle. However, in beef cattle on western ranges, the first indication of a problem is usually finding over 20-40% of the cows open at the time of fall pregnancy check. By this time the significant economic damage has already been done. Treatment efforts need to be directed primarily toward the infected bull rather than the cow because of the self limiting nature of the disease in cows. When trichomoniasis is diagnosed in dairy herds, it can be controlled most effectively by AI. However, in some herds, management levels may be inadequate to have a successful AI program. In these, natural breeding programs must be tailored to eliminate the infection.