bluuebunny/crossref_metadata_2025 huggingface dataset:
Dynamical Analysis of Rubella Disease Model in the Context of Fractional Piecewise Derivative: Simulations with Real Statistical Data
Authors: Alkahtani Badr Saad T.
Published: October 2023

Rubella is a viral disease that can lead to severe health complications, especially in pregnant women and their unborn babies. Understanding the dynamics of the Rubella disease model is crucial for developing effective strategies to control its spread. This paper introduces a major innovation by employing a novel piecewise approach that incorporates two different kernels. This innovative approach significantly enhances the accuracy of modeling Rubella disease dynamics. In the first interval, the Caputo operator is employed to address initial conditions, while the Atangana–Baleanu derivative is utilized in the second interval to account for anomalous diffusion processes. A thorough theoretical analysis of the piecewise derivative for the problem is provided, discussing mathematical properties, stability, and convergence. To solve the proposed problem effectively, the piecewise numerical Newton polynomial technique is employed and the numerical scheme for both kernels is established. Through extensive numerical simulations with various fractional orders, the paper demonstrates the approach’s effectiveness and flexibility in modeling the spread of the Rubella virus. Furthermore, to validate the findings, the simulated results are compared with real data obtained from Rubella outbreaks in Uganda and Tanzania, confirming the practical relevance and accuracy of this innovative model.
Emerging Opportunities to Study Mobile Element Insertions and Their Source Elements in an Expanding Universe of Sequenced Human Genomes
Authors: Devine Scott E.
Published: October 2023

Three mobile element classes, namely Alu, LINE-1 (L1), and SVA elements, remain actively mobile in human genomes and continue to produce new mobile element insertions (MEIs). Historically, MEIs have been discovered and studied using several methods, including: (1) Southern blots, (2) PCR (including PCR display), and (3) the detection of MEI copies from young subfamilies. We are now entering a new phase of MEI discovery where these methods are being replaced by whole genome sequencing and bioinformatics analysis to discover novel MEIs. We expect that the universe of sequenced human genomes will continue to expand rapidly over the next several years, both with short-read and long-read technologies. These resources will provide unprecedented opportunities to discover MEIs and study their impact on human traits and diseases. They also will allow the MEI community to discover and study the source elements that produce these new MEIs, which will facilitate our ability to study source element regulation in various tissue contexts and disease states. This, in turn, will allow us to better understand MEI mutagenesis in humans and the impact of this mutagenesis on human biology.
Improving the Completeness of Chromosome-Level Assembly by Recalling Sequences from Lost Contigs
Authors: Liu Junyang , Liu Fang , Pan Weihua
Published: October 2023

For a long time, the construction of complete reference genomes for complex eukaryotic genomes has been hindered by the limitations of sequencing technologies. Recently, the Pacific Biosciences (PacBio) HiFi data and Oxford Nanopore Technologies (ONT) Ultra-Long data, leveraging their respective advantages in accuracy and length, have provided an opportunity for generating complete chromosome sequences. Nevertheless, for the majority of genomes, the chromosome-level assemblies generated using existing methods still miss a high proportion of sequences due to losing small contigs in the step of assembly and scaffolding. To address this shortcoming, in this paper, we propose a novel method that is able to identify and fill the gaps in the chromosome-level assembly by recalling the sequences in the lost small contigs. Experimental results on both real and simulated datasets demonstrate that this method is able to improve the completeness of the chromosome-level assembly.
Role of Cinema and Psychoanalysis in Affective Experience
Authors: Bellagamba Elisabetta
Published: October 2023

This paper focuses on a peculiar aspect of film viewing, specifically the way in which—interacting with the viewer and their own story—visual images create identifications that can make the viewing a more or less disturbing affective experience. Psychoanalysis and cinema have an indissoluble connection. In particular, the language of cinema comes very close to that of psychoanalysis, given that movies are made according to our psychism. On the basis of this relationship, filmic narration often becomes part of patients’ session, especially teenagers, allowing us to explore areas of the mind hitherto silent.
Perceptions and Experiences of Undergraduate Students Regarding Social Media as a Tool for Government COVID-19-Related Messages: A Qualitative Study in Nottingham, UK
Authors: Sandhu Sanvir , Wilson Emma , Chattopadhyay Kaushik
Published: October 2023

The rise of social media has given way to its use as a form of public health communication. Previous research has shown social media-based interventions to be effective, particularly for university students. Social media was used as a tool for public health messaging during the COVID-19 pandemic; however, there is a lack of information regarding the experiences and perceptions of such messaging and its effectiveness among UK university students. A qualitative study was conducted to explore the perceptions and experiences of public health messaging on social media during the pandemic, as well as the effects of such messaging. Fourteen semi-structured interviews with undergraduate students at the University of Nottingham, UK, were conducted. Five main themes were identified: effects of COVID-19 on university students, use of social media by university students, COVID-19 messaging on social media, effects of public health messaging on social media in compliance with COVID-19 guidelines, and improving public health messaging for the future. This study provides a novel insight into the experiences and perceptions of undergraduate students at a UK university regarding public health messaging on social media during the COVID-19 pandemic.
Implications of Aging in Place in the Context of the Residential Environment: Bibliometric Analysis and Literature Review
Authors: Seo Eugene , Lee Sanghee
Published: October 2023

The residential environment’s impact on aging in place is a multidisciplinary field that draws from architecture, urban planning, gerontology, psychology, and sociology. This multidisciplinary nature makes it challenging to comprehensively understand the field and identify the connections and interactions among disciplines. A bibliometric analysis is crucial for exploring the field’s intellectual structure, identifying interdisciplinary collaborations, and tracking the knowledge flow across disciplines and will facilitate cross-disciplinary dialogue, foster collaboration, and encourage research that integrates diverse perspectives. This study reviewed the literature on aging in place in the context of a residential environment, which required adapting theories and methodologies. It analyzed a dataset of 1500 publications retrieved from the Web of Science, applied performance analysis techniques, and utilized VOSviewer to visualize the intellectual structure and evolving research themes. The results emphasize the increasing strength of academic interest and the growing diversity of fields related to the topic. The findings are discussed in terms of productivity, collaboration, and research themes from the past to the future. The results provide a roadmap for researchers, policymakers, and practitioners worldwide who focus on aging in place and acknowledge the importance of considering the physical, social, and cultural aspects of an older adult’s living environment.
Implementation of Web Map Services for Old Cadastral Maps
Authors: Verdu-Candela Alvaro , Femenia-Ribera Carmen , Mora-Navarro Gaspar , Sierra-Requena Rafael
Published: October 2023

It is widely accepted that old cadastral maps have multiple uses, such as reestablishing cadastral parcel boundaries, municipality boundaries, and coastal limits, or conducting historical, economic, and social studies. In Spain, the Directorate General for Cadastre, and the National Geographic Institute, has numerous digitized old maps that are accessible to users. In the Comunidad Valenciana, the georeferencing of certain series of old cadastral maps is being carried out in phases, which is one of the subjects of this study. A metric analysis of two series of old cadastral maps from a municipality was conducted. One of the series was georeferenced by the Valencia Provincial Cadastre Office, while the other was georeferenced in this research. Additionally, a spatial data infrastructure (SDI) was created, providing WMS, catalog, and document download services. Metadata were also published, containing information about the source, digitalization process, georeferencing, and achieved accuracy, following the ISO 19115 standard for geographic metadata. Furthermore, through individual and group interviews, participatory social research was conducted, to assess the use of old cadastral maps and the created SDI services, aiming to understand the users’ appreciation of the services. The results of the social research indicate that the SDI services created are highly valued, but certain conditions need to be met to ensure their effective use by the general public in order to avoid misuses and misinterpretations.
Question Classification for Intelligent Question Answering: A Comprehensive Survey
Authors: Sun Hao , Wang Shu , Zhu Yunqiang , Yuan Wen , Zou Zhiqiang
Published: October 2023

In the era of GeoAI, Geospatial Intelligent Question Answering (GeoIQA) represents the ultimate pursuit for everyone. Even generative AI systems like ChatGPT-4 struggle to handle complex GeoIQA. GeoIQA is domain complex IQA, which aims at understanding and answering questions accurately. The core of IQA is the Question Classification (QC), which mainly contains four types: content-based, template-based, calculation-based and method-based classification. These IQA_QC frameworks, however, struggle to be compatible and integrate with each other, which may be the bottleneck restricting the substantial improvement of IQA performance. To address this problem, this paper reviewed recent advances on IQA with the focus on solving question classification and proposed a comprehensive IQA_QC framework for understanding user query intention more accurately. By introducing the basic idea of the IQA mechanism, a three-level question classification framework consisting of essence, form and implementation is put forward which could cover the complexity and diversity of geographical questions. In addition, the proposed IQA_QC framework revealed that there are still significant deficiencies in the IQA evaluation metrics in the aspect of broader dimensions, which led to low answer performance, functional performance and systematic performance. Through the comparisons, we find that the proposed IQA_QC framework can fully integrate and surpass the existing classification. Although our proposed classification can be further expanded and improved, we firmly believe that this comprehensive IQA_QC framework can effectively help researchers in both semantic parsing and question querying processes. Furthermore, the IQA_QC framework can also provide a systematic question-and-answer pair/library categorization system for AIGCs, such as GPT-4. In conclusion, whether it is explicit GeoAI or implicit GeoAI, the IQA_QC can play a pioneering role in providing question-and-answer types in the future.
Identification and Functional Analysis of Drought-Responsive Long Noncoding RNAs in Maize Roots
Authors: Tang Xin , Li Qimeng , Feng Xiaoju , Yang Bo , Zhong Xiu , Zhou Yang , Wang Qi , Mao Yan , Xie Wubin , Liu Tianhong , Tang Qi , Guo Wei , Wu Fengkai , Feng Xuanjun , Wang Qingjun , Lu Yanli , Xu Jie
Published: October 2023

Long noncoding RNAs (lncRNAs) are transcripts with lengths of more than 200 nt and limited protein-coding potential. They were found to play important roles in plant stress responses. In this study, the maize drought-tolerant inbred line AC7643 and drought-sensitive inbred line AC7729/TZSRW, as well as their recombinant inbred lines (RILs) were selected to identify drought-responsive lncRNAs in roots. Compared with non-responsive lncRNAs, drought-responsive lncRNAs had different sequence characteristics in length of genes and number of exons. The ratio of down-regulated lncRNAs induced by drought was significantly higher than that of coding genes; and lncRNAs were more widespread expressed in recombination sites in the RILs. Additionally, by integration of the modifications of DNA 5-methylcytidine (5mC), histones, and RNA N6-methyladenosine (m6A), it was found that the enrichment of histone modifications associated with transcriptional activation in the genes generated lncRNAs was lower that coding genes. The lncRNAs-mRNAs co-expression network, containing 15,340 coding genes and 953 lncRNAs, was constructed to investigate the molecular functions of lncRNAs. There are 13 modules found to be associated with survival rate under drought. We found nine SNPs located in lncRNAs among the modules associated with plant survival under drought. In conclusion, we revealed the characteristics of lncRNAs responding to drought in maize roots based on multiomics studies. These findings enrich our understanding of lncRNAs under drought and shed light on the complex regulatory networks that are orchestrated by the noncoding RNAs in response to drought stress.
Differences in the Occurrence of Cell Wall Components between Distinct Cell Types in Glands of Drosophyllum lusitanicum
Authors: Płachno Bartosz J. , Kapusta Małgorzata , Stolarczyk Piotr , Świątek Piotr , Lichtscheidl Irene
Published: October 2023

Carnivorous plants are mixotrophs that have developed the ability to lure, trap, and digest small organisms and utilize components of the digested bodies. Leaves of Drosophyllum lusitanicum have two kinds of glands (emergences): stalked mucilage glands and sessile digestive glands. The stalked mucilage glands perform the primary role in prey lure and trapping. Apart from their role in carnivory, they absorb water condensed from oceanic fog; thus, plants can survive in arid conditions. To better understand the function of carnivorous plant emergences, the molecular composition of their cell walls was investigated using immunocytochemical methods. In this research, Drosophyllum lusitanicum was used as a study system to determine whether cell wall immunocytochemistry differs between the mucilage and digestive glands of other carnivorous plant species. Light and electron microscopy were used to observe gland structure. Fluorescence microscopy revealed the localization of carbohydrate epitopes associated with the major cell wall polysaccharides and glycoproteins. The mucilage gland (emergence) consists of a glandular head, a connecting neck zone, and stalk. The gland head is formed by an outer and inner layer of glandular (secretory) cells and supported by a layer of endodermoid (barrier) cells. The endodermoid cells have contact with a core of spongy tracheids with spiral-shaped thickenings. Lateral tracheids are surrounded by epidermal and parenchymal neck cells. Different patterns of cell wall components were found in the various cell types of the glands. Cell walls of glandular cells generally are poor in both low and highly esterified homogalacturonans (HGs) but enriched with hemicelluloses. Cell walls of inner glandular cells are especially rich in arabinogalactan proteins (AGPs). The cell wall ingrowths in glandular cells are significantly enriched with hemicelluloses and AGPs. In the case of cell wall components, the glandular cells of Drosophyllum lusitanicum mucilage glands are similar to the glandular cells of the digestive glands of Aldrovanda vesiculosa and Dionaea muscipula.
PNPLA3 rs738409 Genetic Variant Inversely Correlates with Platelet Count, Thereby Affecting the Performance of Noninvasive Scores of Hepatic Fibrosis
Authors: Meroni Marica , Dongiovanni Paola
Published: October 2023

Noninvasive tests (NITs) including platelets (PLTs) have been proposed to replace hepatic biopsy for the diagnosis of nonalcoholic fatty liver disease (NAFLD), or as more recently redefined, metabolic dysfunction-associated steatotic liver disease (MASLD). There has been reported an inverse correlation between PLTs and progressive MASLD, which is also affected by the patatin-like phospholipase domain-containing protein 3 (PNPLA3) rs738409 C>G mutation. However, the correlation between low PLTs and PNPLA3 genotype has been poorly investigated. We stratified 1155 biopsy-proven MASLD patients according to PNPLA3 genotype. The hepatic expression of genes involved in megakaryopoiesis was investigated in n = 167 bariatric patients by RNAseq. PLT count progressively decreased according to the number of PNPLA3 at-risk alleles, irrespective of the presence of advanced fibrosis. The hepatic expression of genes involved in PLT biogenesis was associated with the PNPLA3 GG genotype. Finally, the presence of the PNPLA3 homozygosity flattened the accuracy of fibrosis-4 (FIB-4) in discriminating histological fibrosis stages. The PNPLA3 GG genotype may underpower the accuracy of NITs which include PLT count in identifying those patients with potentially reversible stages of fibrosis.
2-Aminothiazole-Flavonoid Hybrid Derivatives Binding to Tau Protein and Responsible for Antitumor Activity in Glioblastoma
Authors: Hedna Rayane , DiMaio Attilio , Robin Maxime , Allegro Diane , Tatoni Mario , Peyrot Vincent , Barbier Pascale , Kovacic Hervé , Breuzard Gilles
Published: October 2023

Tau protein has been described for several decades as a promoter of tubulin assembly into microtubules. Dysregulation or alterations in Tau expression have been related to various brain cancers, including the highly aggressive and lethal brain tumor glioblastoma multiform (GBM). In this respect, Tau holds significant promise as a target for the development of novel therapies. Here, we examined the structure–activity relationship of a new series of seventeen 2-aminothiazole-fused to flavonoid hybrid compounds (TZF) on Tau binding, Tau fibrillation, and cellular effects on Tau-expressing cancer cells. By spectrofluorometric approach, we found that two compounds, 2 and 9, demonstrated high affinity for Tau and exhibited a strong propensity to inhibit Tau fibrillation. Then, the biological activity of these compounds was evaluated on several Tau-expressing cells derived from glioblastoma. The two lead compounds displayed a high anti-metabolic activity on cells related to an increased fission of the mitochondria network. Moreover, we showed that both compounds induced microtubule bundling within newly formed neurite-like protrusions, as well as with defection of cell migration. Taken together, our results provide a strong experimental basis to develop new potent molecules targeting Tau-expressing cancer cells, such as GBM.
Structure–Chiral Selectivity Relationships of Various Mandelic Acid Derivatives on Octakis 2,3-di-O-acetyl-6-O-tert-butyldimethylsilyl-gamma-cyclodextrin Containing Gas Chromatographic Stationary
Authors: Repassy Levente , Juvancz Zoltan , Bodane-Kendrovics Rita , Kaleta Zoltan , Hunyadi Csaba , Riszter Gergo
Published: October 2023

Frequently, a good chiral separation is the result of long trial and error processes. The three-point interaction mechanisms require the fair geometrical fitting and functional group compatibility of the interacting groups. Structure–chiral selectivity correlations are guidelines that can be established via trough systematic studies using model compounds. The enantiorecognition of the test compounds was studied on an octakis 2,3-Di-O-acetyl-6-O-tert-butyldimethylsilyl-gamma-cyclodextrin (TBDMSDAGCD) chiral selector. In our work, mandelic acid and its variously substituted compounds were used as model compounds to establish adaptable rules for other enantiomeric pairs. The mandelic acid and its modified compounds were altered at both their carboxyl and hydroxyl positions to test the key interaction forces of the chiral recognition processes. Ring- and alkyl-substituted mandelic acid derivatives were also used in our experiments. The chiral selectivity values of 20 test compounds were measured and extrapolated to 100 °C. The hydrogen donor abilities of test compounds improved their chiral selectivities. The inclusion phenomenon also played a role in chiral recognition processes in several cases. Enantiomer elution reversals were observed for different derivatives of hydroxyl groups, providing evidence for the multimodal character of the selector. The results of our research can serve as guidelines to achieve appropriate chiral separation for other enantiomeric pairs.
Overexpression of ApHIPP26 from the Hyperaccumulator Arabis paniculata Confers Enhanced Cadmium Tolerance and Accumulation to Arabidopsis thaliana
Authors: Zhou Lizhou , Ye Lvlan , Pang Biao , Hou Yunyan , Yu Junxing , Du Xuye , Gu Lei , Wang Hongcheng , Zhu Bin
Published: October 2023

Cadmium (Cd) is a toxic heavy metal that seriously affects metabolism after accumulation in plants, and it also causes adverse effects on humans through the food chain. The HIPP gene family has been shown to be highly tolerant to Cd stress due to its special domain and molecular structure. This study described the Cd-induced gene ApHIPP26 from the hyperaccumulator Arabis paniculata. Its subcellular localization showed that ApHIPP26 was located in the nucleus. Transgenic Arabidopsis overexpressing ApHIPP26 exhibited a significant increase in main root length and fresh weight under Cd stress. Compared with wild-type lines, Cd accumulated much more in transgenic Arabidopsis both aboveground and underground. Under Cd stress, the expression of genes related to the absorption and transport of heavy metals underwent different changes in parallel, which were involved in the accumulation and distribution of Cd in plants, such as AtNRAMP6 and AtNRAMP3. Under Cd stress, the activities of antioxidant enzymes (superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase) in the transgenic lines were higher than those in the wild type. The physiological and biochemical indices showed that the proline and chlorophyll contents in the transgenic lines increased significantly after Cd treatment, while the malondialdehyde (MDA) content decreased. In addition, the gene expression profile analysis showed that ApHIPP26 improved the tolerance of Arabidopsis to Cd by regulating the changes of related genes in plant hormone signal transduction pathway. In conclusion, ApHIPP26 plays an important role in cadmium tolerance by alleviating oxidative stress and regulating plant hormones, which provides a basis for understanding the molecular mechanism of cadmium tolerance in plants and provides new insights for phytoremediation in Cd-contaminated areas.
The Responses of Sucrose Metabolism and Carbon Translocation in Tomato Seedlings under Different Light Spectra
Authors: Zhan Xiaoxu , Yang Qichang , Wang Sen , Wang Yu , Fan Xiaoxue , Bian Zhonghua
Published: October 2023

Light plays a dominant role in the biosynthesis and accumulation of photosynthetic products. However, the metabolism and translocation of photosynthetic products in plants under different light spectra remain elusive. In this study, tomato (Solanum lycopersicum L.) seedlings were treated with different light spectra delivered by light-emitting diodes (LEDs) with the same photosynthetic photon flux density at 300 μmol m−2 s−1, including monochromatic red (660 nm, R), blue (450 nm, B), sun-like white (W, 380–780 nm), or a combination of R and B lights (R:B = 1:1, RB). Compared with W, the biomass distribution ratio for leaves under R, B, and RB decreased by 5.01–9.53%, while the ratio for stems and roots increased by 3.71–6.92% and 0.14–2.81%, respectively. The photosynthetic carbon distribution expressed as 13C enrichment was higher in stems and roots under RB and R, while B led to more 13C transported from leaves and enriched in stems when compared with W. Meanwhile, RB led to significant increases in the activities of phosphate synthase (SPS), sucrose synthase (SS), vacuolar acid invertase (VI), and neutral invertase (NI). The R was more efficient in increasing the activity of SPS and SS, while B was more effective in promoting the activity of VI and NI. The transcript levels of SPS, SS3, NI6, and VI were upregulated under R, B, and RB. However, the transcript patterns of SPS, SS3, NI6, and VI were not consistent with the changes in their encoded enzymes, especially the transcript patterns of SPS and SS3. Our study suggests that the red- and blue-light-induced long-distance and short-distance transport of photosynthetic products in plants, respectively, might result from different regulation of sucrose-metabolizing enzymes from transcriptional and post-transcriptional levels.
A Newborn Screening Program for Sickle Cell Disease in Murcia (Spain)
Authors: Sánchez-Villalobos María , Campos Baños Eulalia , Juan Fita María Jesús , Egea Mellado José María , Gonzalez Gallego Inmaculada , Beltrán Videla Asunción , Berenguer Piqueras Mercedes , Bermúdez Cortés Mar , Moraleda Jiménez José María , Guillen Navarro Encarna , Salido Fierrez Eduardo , Pérez-Oliva Ana B.
Published: October 2023

Sickle cell disease (SCD) is an inherited autosomal recessive hemoglobin disorder caused by the presence of hemoglobin S, a mutant abnormal hemoglobin caused by a nucleotide change in codon 6 of the β-globin chain gene. SCD involves a chronic inflammatory state, exacerbated during vaso-occlusive crises, which leads to end-organ damage that occurs throughout the lifespan. SCD is associated with premature mortality in the first years of life. The process of sickling provokes asplenia in the first years of life with an increased risk of infection by encapsulated germs. These complications can be life-threatening and require early diagnosis and management. The most important interventions recommend an early diagnosis of SCD to ensure that affected newborns receive immediate care to reduce mortality and morbidity. The newborn screening program in the region of Murcia for SCD began in March 2016. We aimed to determine the incidence of sickle cell anemia and other structural hemoglobinopathies in the neonatal population of the region of Murcia, an area of high migratory stress, and to systematically assess the benefit of newborn screening for SCD, leading to earlier treatment, as well as to offer genetic counseling to all carriers. The prevalence of SCD in our region is similar to others in Spain, except for Catalonia and Madrid. The newborns with confirmed diagnoses of SCD received early attention, and all the carriers received genetic counseling.
A Survey of Machine Learning Assisted Continuous-Variable Quantum Key Distribution
Authors: Long Nathan K. , Malaney Robert , Grant Kenneth J.
Published: October 2023

Continuous-variable quantum key distribution (CV-QKD) shows potential for the rapid development of an information-theoretic secure global communication network; however, the complexities of CV-QKD implementation remain a restrictive factor. Machine learning (ML) has recently shown promise in alleviating these complexities. ML has been applied to almost every stage of CV-QKD protocols, including ML-assisted phase error estimation, excess noise estimation, state discrimination, parameter estimation and optimization, key sifting, information reconciliation, and key rate estimation. This survey provides a comprehensive analysis of the current literature on ML-assisted CV-QKD. In addition, the survey compares the ML algorithms assisting CV-QKD with the traditional algorithms they aim to augment, as well as providing recommendations for future directions for ML-assisted CV-QKD research.
Lateralized Movements during the Mating Behavior, Which Are Associated with Sex and Sexual Experience, Increase the Mating Success in Alphitobius diaperinus (Coleoptera: Tenebrionidae)
Authors: Calla-Quispe Erika , Irigoin Esperanza , Mansurova Madina , Martel Carlos , Ibáñez Alfredo J.
Published: October 2023

In the present study, we explored the effects of displacement directionality in mating behavior (i.e., lateralized and non-lateralized movements) on mating success (i.e., copulation occurs) and efficiency (i.e., time length at which copulation is achieved), and its association with sex and sexual experience in A. diaperinus. To do so, we carried out mating experiments and recorded the behavior of the mating pair during the whole mating sequence (i.e., precopulatory and copulatory phases). During the precopulatory phase, independently of sex and sexual experience, all beetles performed non-lateralized (i.e., backside or frontside) approaches; however, only sexually experienced beetles showed lateralized approaches (i.e., right-side and left-side). Notably, experienced males exhibited greater mating success than virgin males. After the approach, both virgin and experienced males displayed lateralized and non-lateralized mounts on the females with distinct mating success. Regardless of their sexual experience, 100% of successful mating attempts were achieved when males mounted from the females’ right side. Furthermore, the development of lateralized approaches and mounts reduces the time of mating sequence span compared with non-lateralized behaviors. We highlight the importance of lateralization in mating behavior and sexual experience to achieve higher mating success, addressing a potential learning ability of beetles based on experience.
Recommendations for the Clinical Approach to Immune Thrombocytopenia: Spanish ITP Working Group (GEPTI)
Authors: Mingot-Castellano María , Canaro Hirnyk Mariana , Sánchez-González Blanca , Álvarez-Román María , Bárez-García Abelardo , Bernardo-Gutiérrez Ángel , Bernat-Pablo Silvia , Bolaños-Calderón Estefanía , Butta-Coll Nora , Caballero-Navarro Gonzalo , Caparrós-Miranda Isabel , Entrena-Ureña Laura , Fernández-Fuertes Luis , García-Frade Luis , Gómez del Castillo María , González-López Tomás , Grande-García Carlos , Guinea de Castro José , Jarque-Ramos Isidro , Jiménez-Bárcenas Reyes , López-Ansoar Elsa , Martínez-Carballeira Daniel , Martínez-Robles Violeta , Monteagudo-Montesinos Emilio , Páramo-Fernández José , Perera-Álvarez María , Soto-Ortega Inmaculada , Valcárcel-Ferreiras David , Pascual-Izquierdo Cristina
Published: October 2023

Primary immune thrombocytopenia (ITP) is a complex autoimmune disease whose hallmark is a deregulation of cellular and humoral immunity leading to increased destruction and reduced production of platelets. The heterogeneity of presentation and clinical course hampers personalized approaches for diagnosis and management. In 2021, the Spanish ITP Group (GEPTI) of the Spanish Society of Hematology and Hemotherapy (SEHH) updated a consensus document that had been launched in 2011. The updated guidelines have been the reference for the diagnosis and management of primary ITP in Spain ever since. Nevertheless, the emergence of new tools and strategies makes it advisable to review them again. For this reason, we have updated the main recommendations appropriately. Our aim is to provide a practical tool to facilitate the integral management of all aspects of primary ITP management.
A Review of FLT3 Kinase Inhibitors in AML
Authors: Negotei Cristina , Colita Andrei , Mitu Iuliana , Lupu Anca Roxana , Lapadat Mihai-Emilian , Popovici Constanta Elena , Crainicu Madalina , Stanca Oana , Berbec Nicoleta Mariana
Published: October 2023

Acute myeloid leukemia (AML) is a highly aggressive illness distinguished by the accumulation of abnormal hematopoietic precursors in both the bone marrow and peripheral blood. The prevalence of FLT3 gene mutations is high and escalates the probability of relapse and mortality. The survival rates for AML patients, particularly those over 65, are low. FLT3 mutation screening at diagnosis is mandatory, and FLT3 inhibitors are crucial in treating AML patients with mutations. There are two categories of FLT3 mutations: FLT3-ITD located in the juxtamembrane domain and FLT3-TKD in the tyrosine kinase domain. FLT3-ITD is the most common type, affecting nearly a quarter of patients, whereas FLT3-TKD only affects 6–8% of patients. FLT3 inhibitors are now crucial in treating AML patients with FLT3 mutations. When dealing with FLT3-mutated AML, the recommended course of treatment typically involves chemotherapy and midostaurin, followed by allogeneic hematopoietic cell transplantation (HCT) to maximize the likelihood of success. Maintenance therapy can lower the risk of relapse, and gilteritinib is a better option than salvage chemotherapy for relapsed or refractory cases. Clinical trials for new or combined therapies are the most effective approach. This review discusses treatment options for patients with FLT3-mutated AML, including induction chemotherapy and options for relapsed or refractory disease. Additional treatment options may become available as more studies are conducted based on the patient’s condition and susceptibility.
Increasing Soluble P-Selectin Levels Predict Higher Peripheral Atherosclerotic Plaque Progression
Authors: Sommer Philip , Schreinlechner Michael , Noflatscher Maria , Lener Daniela , Mair Fabian , Theurl Markus , Kirchmair Rudolf , Marschang Peter
Published: October 2023

Background and aims: The adhesion molecule P-selectin is expressed by endothelial cells and platelets. It is involved in platelet activation and leukocyte adhesion, both important processes in the pathogenesis of atherosclerosis. Our study was designed to assess the predictive value of soluble P-selectin (sP-selectin) on the progression of peripheral atherosclerosis. Methods: This is an observational, single-center, cohort study that included 443 patients with established cardiovascular disease (CVD) or at least one cardiovascular risk factor. Over a period of 4 years, each patient underwent three-dimensional (3D) ultrasound to assess the plaque volume of the carotid and femoral arteries once per year. In addition, plasma sP-selectin levels were measured at each visit. The association between changes in sP-selectin and peripheral atherosclerotic plaque progression was assessed using growth curve models. Results: 338 patients were available for statistical analysis. Each standard deviation increase in sP-selectin was significantly (p < 0.001) associated with a 46.09 mm3 higher plaque volume. In ROC-analysis, changes in sP-selectin over time showed an optimal cut-off value around Δ 0.0 µg/mL sP-selectin and significantly improved the predictive value of the ESC-SCORE (AUC for the combination of both parameters was 0.75 (95% CI 0.68–0.81, p < 0.001). Patients with increasing sP-selectin showed a significantly higher plaque progression compared to patients with decreasing or stable sP-selectin levels (202 mm3 vs. 110 mm3, p < 0.001). Conclusions: Increasing sP-selectin levels can predict higher atherosclerotic plaque progression as measured by 3D ultrasound. We suggest serial measurements of sP-selectin as an easily measurable biomarker for peripheral atherosclerotic plaque progression.
Knee Cartilage Lesion Management—Current Trends in Clinical Practice
Authors: Jarecki Jaromir , Waśko Marcin Krzysztof , Widuchowski Wojciech , Tomczyk-Warunek Agnieszka , Wójciak Magdalena , Sowa Ireneusz , Blicharski Tomasz
Published: October 2023

Many patients, particularly those aged above 40, experience knee joint pain, which hampers both sports activities and daily living. Treating isolated chondral and osteochondral defects in the knee poses a significant clinical challenge, particularly in younger patients who are not typically recommended partial or total knee arthroplasty as alternatives. Several surgical approaches have been developed to address focal cartilage defects. The treatment strategies are characterized as palliation (e.g., chondroplasty and debridement), repair (e.g., drilling and microfracture), or restoration (e.g., autologous chondrocyte implantation, osteochondral autograft, and osteochondral allograft). This review offers an overview of the commonly employed clinical methods for treating articular cartilage defects, with a specific focus on the clinical trials conducted in the last decade. Our study reveals that, currently, no single technology fully meets the essential requirements for effective cartilage healing while remaining easily applicable during surgical procedures. Nevertheless, numerous methods are available, and the choice of treatment should consider factors such as the location and size of the cartilage lesion, patient preferences, and whether it is chondral or osteochondral in nature. Promising directions for the future include tissue engineering, stem cell therapies, and the development of pre-formed scaffolds from hyaline cartilage, offering hope for improved outcomes.
Temporal Changes in the Skin Microbiome of Epidermolysis Bullosa Patients following the Application of Wound Dressings
Authors: Horev Amir , Brandwein Michael , Vaknin Avraham , Motro Yair , Moran-Gilad Jacob
Published: October 2023

Objective: Epidermolysis bullosa (EB) is a group of rare hereditary skin disorders characterized by the formation of painful blisters, erosions, and ulcers. In addition, the wounds can easily become infected with different pathogens. Therefore, the dynamics in the microbial populations across the various stages of EB can shed light on pathophysiology, the effect of treatment, and the factors involved in its recovery, but they are understudied. We thus sought to characterize the skin microbiome among patients with EB over time. Methods: A prospective study conducted in the pediatric dermatology clinic at Soroka Medical Center, Beer-Sheva, Israel. Children (0–18) with simplex and recessive dystrophic EB were sampled at two different time points: before a therapeutic regimen and 90 days (±14 days) later. Samples were obtained from lesional skin (wound), healthy, non-lesional skin, and seborrheic skin (forehead). Samples were subject to 16S rRNA amplicon sequencing. Analyses performed included comparisons of relative abundance at the phyla and genera taxonomic levels, alpha and beta diversity comparisons, and differential abundance. Results: 32 children with EB were enrolled, for whom 192 skin microbiome samples were obtained. Lesional skin samples harbored significantly less Bacteroidota and Fusobacteriota before the initiation of treatment. Following topical dressing, we observed more Firmicutes and less Proteobacteria in lesional skin samples than healthy and seborrheic skin samples. In addition, Staphylococcus was significantly more abundant in lesional samples than in non-lesional and seborrheic samples following treatment. Conclusions: Our study recaptured the reduced bacterial diversity and increased staphylococcal carriage in EB patients, showing a potential effect of topical dressing either directly on the wound microbiome or indirectly through the contribution towards skin healing. The detection of Firmicutes in general, and S. aureus specifically, commensurate with the application of a wound dressing may warrant the use of additional treatment methods to facilitate wound healing. Future studies in these patients should prospectively correlate the temporal changes in the microbiome associated with various treatment modalities in order to optimize the care of EB patients.
Gastric Alimetry® Test Interpretation in Gastroduodenal Disorders: Review and Recommendations
Authors: Foong Daphne , Calder Stefan , Varghese Chris , Schamberg Gabriel , Xu William , Daker Charlotte , Ho Vincent , Andrews Christopher N. , Gharibans Armen A. , O’Grady Greg
Published: October 2023

Chronic gastroduodenal symptoms are prevalent worldwide, and there is a need for new diagnostic and treatment approaches. Several overlapping processes may contribute to these symptoms, including gastric dysmotility, hypersensitivity, gut–brain axis disorders, gastric outflow resistance, and duodenal inflammation. Gastric Alimetry® (Alimetry, New Zealand) is a non-invasive test for evaluating gastric function that combines body surface gastric mapping (high-resolution electrophysiology) with validated symptom profiling. Together, these complementary data streams enable important new clinical insights into gastric disorders and their symptom correlations, with emerging therapeutic implications. A comprehensive database has been established, currently comprising > 2000 Gastric Alimetry tests, including both controls and patients with various gastroduodenal disorders. From studies employing this database, this paper presents a systematic methodology for Gastric Alimetry test interpretation, together with an extensive supporting literature review. Reporting is grouped into four sections: Test Quality, Spectral Analysis, Symptoms, and Conclusions. This review compiles, assesses, and evaluates each of these aspects of test assessment, with discussion of relevant evidence, example cases, limitations, and areas for future work. The resultant interpretation methodology is recommended for use in clinical practice and research to assist clinicians in their use of Gastric Alimetry as a diagnostic aid and is expected to continue to evolve with further development.
The Effects of Heparan Sulfate Infusion on Endothelial and Organ Injury in a Rat Pneumosepsis Model
Authors: van den Brink Daan , Kleinveld Derek , Bongers Annabel , Vos Jaël , Roelofs Joris , Weber Nina , van Buul Jaap , Juffermans Nicole
Published: October 2023

Septic shock is characterized by endothelial dysfunction, leading to tissue edema and organ failure. Heparan sulfate (HS) is essential for vascular barrier integrity, possibly via albumin as a carrier. We hypothesized that supplementing fluid resuscitation with HS would improve endothelial barrier function, thereby reducing organ edema and injury in a rat pneumosepsis model. Following intratracheal inoculation with Streptococcus pneumoniae, Sprague Dawley rats were randomized to resuscitation with a fixed volume of either Ringer’s Lactate (RL, standard of care), RL supplemented with 7 mg/kg HS, 5% human albumin, or 5% human albumin supplemented with 7 mg/kg HS (n = 11 per group). Controls were sham inoculated animals. Five hours after the start of resuscitation, animals were sacrificed. To assess endothelial permeability, 70 kD FITC-labelled dextran was administered before sacrifice. Blood samples were taken to assess markers of endothelial and organ injury. Organs were harvested to quantify pulmonary FITC-dextran leakage, organ edema, and for histology. Inoculation resulted in sepsis, with increased lactate levels, pulmonary FITC-dextran leakage, pulmonary edema, and pulmonary histologic injury scores compared to healthy controls. RL supplemented with HS did not reduce median pulmonary FITC-dextran leakage compared to RL alone (95.1 CI [62.0–105.3] vs. 87.1 CI [68.9–139.3] µg/mL, p = 0.76). Similarly, albumin supplemented with HS did not reduce pulmonary FITC-dextran leakage compared to albumin (120.0 [93.8–141.2] vs. 116.2 [61.7 vs. 160.8] µg/mL, p = 0.86). No differences were found in organ injury between groups. Heparan sulfate, as an add-on therapy to RL or albumin resuscitation, did not reduce organ or endothelial injury in a rat pneumosepsis model. Higher doses of heparan sulfate may decrease organ and endothelial injury induced by shock.
The Effect of Cataract Surgery on the Risk of Dementia: A Nationwide Cohort Study
Authors: Lee Chaeyeon , Shin Eunhae , Kim Mina , Bae Yoonjong , Chung Tae-Young , Seo Sang Won , Jang Hyemin , Lim Dong Hui
Published: October 2023

Background: An advanced age and the female sex are widely recognized risk factors for both cataract and dementia. We investigated the effect of cataract surgery on the incidence of dementia in a Korean population aged ≥ 45 years with a previous diagnosis of cataract. Methods: This nationwide cohort study was performed using Korean National Health Insurance Service data collected from 2006 to 2017. A total of 300,327 subjects aged ≥ 45 years with a history of cataract diagnosis but no previous diagnosis of dementia were analyzed. The relationship between cataract surgery and dementia was evaluated, applying a time-varying analysis to evaluate the hazard ratio (HR) and 95% confidence interval (CI) values according to dementia. It was calculated via a multivariable Cox regression model, with adjustments for age, sex, visual acuity (VA), ocular and systemic comorbidities, and social factors (including body mass index, income, smoking, and drinking). Results: In the multivariate analysis, the cataract surgery group showed a marginal difference in dementia development (HR 1.10 [95% CI 1.02–1.19]) because both cataract and dementia share common risk factors. However, in the subgroup analysis, men (HR 0.49 [95% CI 0.26–0.90]) and patients under 65 years of age (HR 0.88 [95% CI 0.79–0.99]) in the group with cataract surgery and good VA showed a significantly lower incidence of dementia. Conclusion: Through visual improvement, together with timely surgical intervention, the procedure can alleviate the risk of dementia in visually impaired patients, especially in younger and male patients.
Factors Associated with Periodontitis in Younger Individuals: A Scoping Review
Authors: Hung Man , Kelly Roah , Mohajeri Amir , Reese Logan , Badawi Sarah , Frost Cole , Sevathas Taroniar , Lipsky Martin S.
Published: October 2023

Periodontitis is a disease that affects many young adults, and if left untreated, it can have lasting and permanent effects on an individual’s oral health. The purpose of this scoping review was to review the recent literature to identify factors that place young individuals at risk of stage II or III periodontitis. Using the PRISMA guidelines for scoping reviews, three databases were systematically searched for peer-reviewed human studies published in English that investigated risk factors associated with stage II and/or III periodontitis in individuals less than 40 years of age. This review excluded abstracts, literature reviews, including narrative, scoping, and systematic reviews and meta-analyses, conference proceedings, letters to the editor, and editorials. The authors then extracted data from the relevant studies using a predefined form to summarize the aims, design, results, risk factors examined, and the type and severity of periodontitis. Among a total of 2676 articles screened, only three articles met the review’s inclusion criteria. Of these articles, one was a longitudinal case-control study and two were cross-sectional studies. Identified risk factors associated with stage II or III periodontitis included self-reported bleeding when brushing, low bone mineral density, being overweight, and smoking in young adults. Of note, only three studies met the inclusion criteria, suggesting a gap in the research literature.