bluuebunny/crossref_metadata_2025 huggingface dataset:
Magmatic and tectonic segmentation of the intermediate-spreading Costa Rica Rift—a fine balance between magma supply rate, faulting and hydrothermal circulation
Authors: Robinson A H , Zhang L , Hobbs R W , Peirce C , Tong V C H
Published: March 2020

3-D tomographic modelling of wide-angle seismic data, recorded at the intermediate-spreading Costa Rica Rift, has revealed a P-wave seismic velocity anomaly low located beneath a small overlapping spreading centre that forms a non-transform discontinuity at the ridge axis. This low velocity zone displays a maximum velocity anomaly relative to the ‘background’ ridge axis crustal structure of ∼0.5 km s−1, has lateral dimensions of ∼10 × 5 km, and extends to depths ≥2.5 km below the seabed, placing it within layer 2 of the oceanic crust. We interpret these observations as representing increased fracturing under enhanced tectonic stress associated with the opening of the overlapping spreading centre, that results in higher upper crustal bulk porosity and permeability. Evidence for ongoing magmatic accretion at the Costa Rica Rift ridge axis takes the form of an axial magma lens beneath the western ridge segment, and observations of hydrothermal plume activity and microearthquakes support the presence of an active fluid circulation system. We propose that fracture pathways associated with the low velocity zone may provide the system through which hydrothermal fluids circulate. These fluids cause rapid cooling of the adjacent ridge axis and any magma accumulations which may be present. The Costa Rica Rift exists at a tipping point between episodic phases of magmatic and tectonically enhanced spreading. The characteristics inherited from each spreading mode have been preserved in the crustal morphology off-axis for the past 7 Myr. Using potential field data, we contextualize our seismic observations of the axial ridge structure at the whole segment scale, and find that the proposed balance between magmatic and tectonically dominated spreading processes observed off-axis may also be apparent along-axis, and that the current larger-scale magma supply system at the Costa Rica Rift may be relatively weak. Based on all available geophysical observations, we suggest a model for the inter-relationships between magmatism, faulting and fluid circulation at the Costa Rica Rift across a range of scales, which may also be influenced by large lithosphere scale structural and/or thermal heterogeneity.
Common variation at 16p11.2 is associated with glycosuria in pregnancy: findings from a genome-wide association study in European women
Authors: Lee Matthew A , McMahon George , Karhunen Ville , Wade Kaitlin H , Corbin Laura J , Hughes David A , Smith George Davey , Lawlor Debbie A , Jarvelin Marjo-Riitta , Timpson Nicholas J
Published: March 2020

Glycosuria is a condition where glucose is detected in urine at higher concentrations than normal (i.e. not detectable). Glycosuria at some point during pregnancy has an estimated prevalence of 50% and is associated with adverse outcomes in both mothers and offspring. Little is currently known about the genetic contribution to this trait or the extent to which it overlaps with other seemingly related traits, e.g. diabetes. We performed a genome-wide association study (GWAS) for self-reported glycosuria in pregnant mothers from the Avon Longitudinal Study of Parents and Children (cases/controls = 1249/5140). We identified two loci, one of which (lead SNP = rs13337037; chromosome 16; odds ratio of glycosuria per effect allele: 1.42; 95% CI: 1.30, 1.56; P = 1.97 × 10−13) was then validated using an obstetric measure of glycosuria measured in the same cohort (227/6639). We performed a secondary GWAS in the 1986 Northern Finland Birth Cohort (NFBC1986; 747/2991) using midwife-reported glycosuria and offspring genotype as a proxy for maternal genotype. The combined results revealed evidence for a consistent effect on glycosuria at the chromosome 16 locus. In follow-up analyses, we saw little evidence of shared genetic underpinnings with the exception of urinary albumin-to-creatinine ratio (Rg = 0.64; SE = 0.22; P = 0.0042), a biomarker of kidney disease. In conclusion, we identified a genetic association with self-reported glycosuria during pregnancy, with the lead SNP located 15kB upstream of SLC5A2, a target of antidiabetic drugs. The lack of strong genetic correlation with seemingly related traits such as type 2 diabetes suggests different genetic risk factors exist for glycosuria during pregnancy.
Transcriptome changes during the initiation and progression of Duchenne muscular dystrophy in Caenorhabditis elegans
Authors: Hrach Heather C , O’Brien Shannon , Steber Hannah S , Newbern Jason , Rawls Alan , Mangone Marco
Published: March 2020

Duchenne muscular dystrophy (DMD) is a lethal, X-linked disease characterized by progressive muscle degeneration. The condition is driven by nonsense and missense mutations in the dystrophin gene, leading to instability of the sarcolemma and skeletal muscle necrosis and atrophy. Resulting changes in muscle-specific gene expression that take place in dystrophin’s absence remain largely uncharacterized, as they are potentially obscured by the chronic inflammation elicited by muscle damage in humans. Caenorhabditis elegans possess a mild inflammatory response that is not active in the muscle, and lack a satellite cell equivalent. This allows for the characterization of the transcriptome rearrangements affecting disease progression independently of inflammation and regeneration. In effort to better understand these dynamics, we have isolated and sequenced body muscle-specific transcriptomes from C. elegans lacking functional dystrophin at distinct stages of disease progression. We have identified an upregulation of genes involved in mitochondrial function early in disease progression, and an upregulation of genes related to muscle repair in later stages. Our results suggest that in C. elegans, dystrophin may have a signaling role early in development, and its absence may activate compensatory mechanisms that counteract muscle degradation caused by loss of dystrophin. We have also developed a temperature-based screening method for synthetic paralysis that can be used to rapidly identify genetic partners of dystrophin. Our results allow for the comprehensive identification of transcriptome changes that potentially serve as independent drivers of disease progression and may in turn allow for the identification of new therapeutic targets for the treatment of DMD.
Aquaculture-driven evolution: distribution of pyrethroid resistance in the salmon louse throughout the North Atlantic in the years 2000–2017
Authors: Fjørtoft Helene Børretzen , Nilsen Frank , Besnier Francois , Espedal Per Gunnar , Stene Anne , Tveten Ann-Kristin , Bjørn Pål Arne , Aspehaug Vidar Teis , Glover Kevin Alan
Published: March 2020

The parasitic salmon louse, and its documented resistance to chemotherapeutants, represents the most persistent environmental challenge to global salmonid aquaculture. We used a genetic marker associated with pyrethroid resistance to analyse ∼15 000 lice collected from the North Atlantic in the period 2000–2017. The genotype associated with resistance was not detected in lice collected from throughout the North Atlantic in the year 2000 or 2002. However, by the year 2009 onwards, it was found in lice from fish farms throughout much of the North Atlantic. It was also found in modest frequencies in lice collected from wild Atlantic salmon captured off Greenland. The most recent samples displayed very high frequencies of the genotype associated with resistance, particularly in intensive aquaculture regions of Norway (>90%) and Scotland (>70%). These results closely align with observations from the field. We suggest that pyrethroid resistance first emerged in Europe just before or around the year 2000 and was thereafter dispersed throughout much of the North Atlantic where its increased frequency was driven by extensive pyrethroid use. Although the resistant genotype was not detected in lice from Canada, it is likely to occur in very low frequencies that would quickly increase if pyrethroids were to be used in that region.
Mind the gap between ICES nations’ future seafood consumption and aquaculture production
Authors: Froehlich Halley E , Couture Jessica , Falconer Lynne , Krause Gesche , Morris James A , Perez Montse , Stentiford Grant D , Vehviläinen Harri , Halpern Benjamin S
Published: March 2020

As the human population grows and climate change threatens the stability of seafood sources, we face the key question of how we will meet increasing demand, and do so sustainably. Many of the 20 International Council for the Exploration of the Sea (ICES) member nations have been global leaders in the protection and management of wild fisheries, but to date, most of these nations have not developed robust aquaculture industries. Using existing data and documentation of aquaculture targets from government and industry, we compiled and analysed past trends in farmed and wild seafood production and consumption in ICES nations, as well as the potential and need to increase aquaculture production by 2050. We found that the majority of ICES nations lacks long-term strategies for aquaculture growth, with an increasing gap between future domestic production and consumption—resulting in a potential 7 million tonne domestic seafood deficit by 2050, which would be supplemented by imports from other countries (e.g. China). We also found recognition of climate change as a concern for aquaculture growth, but little on what that means for meeting production goals. Our findings highlight the need to prioritize aquaculture policy to set more ambitious domestic production goals and/or improve sustainable sourcing of seafood from other parts of the world, with explicit recognition and strategic planning for climate change affecting such decisions. In short, there is a need for greater concerted effort by ICES member nations to address aquaculture’s long-term future prospects.
Thermal properties of sonicated graphene in coconut oil as a phase change material for energy storage in building applications1
Authors: Safira Lulu , Putra Nandy , Trisnadewi Titin , Kusrini Eny , Mahlia Teuku Meurah Indra
Published: March 2020

This study aims to investigate the thermal properties of a phase change material (PCM) based on coconut oil for building energy storage applications. Coconut oil is classified as an organic PCM composed of fatty acids made from renewable feedstock. However, low thermal conductivity is one of the major drawbacks of organic PCMs that must be improved. Graphene could be an effective material to enhance the thermal performance of organic PCMs. In this study, coconut oil with a latent heat capacity of 114.6 J/g and a melting point of 17.38°C was used. PCMs were prepared by sonicating graphene into coconut oil, as a supporting material. The mass fractions of the prepared PCMs were 0, 0.1, 0.2, 0.3, 0.4 and 0.5. Thermal conductivity tests were performed using a KD2 thermal property analyser under different ambient temperatures of 5, 10, 15, 20 and 25°C simulated with a circulating thermostatic bath. The latent heat, melting point and freezing point were determined through differential scanning calorimetry, the thermal stability was determined using thermogravimetric analysis (TGA) and the morphology and chemical structure were examined using transmission electron microscopy and Fourier-transform infrared spectroscopy, respectively. The results of this study showed that graphene addition to coconut oil improved the thermal performance, with the highest improvement seen in a 0.3 wt% sample at 20°C. The latent heat decreased by 11% owing to molecular movements within the PCM. However, TGA revealed that the composite PCMs showed good thermal stability in ambient building temperature ranges.
Interactions Between Antenatal Sulfadoxine-Pyrimethamine, Drug-Resistant Plasmodium falciparum Parasites, and Delivery Outcomes in Malawi
Authors: Taylor Steve M , Levitt Brandt , Freedman Betsy , Madanitsa Mwayiwawo , Thwai Kyaw-Lay , Kalilani-Phiri Linda , Khairallah Carole , Mwapasa Victor , ter Kuile Feiko O , Meshnick Steven R
Published: March 2020

Background

Sulfadoxine-pyrimethamine (SP) is used as intermittent preventive therapy in pregnancy (IPTp) for malaria in sub-Saharan Africa. The resistance marker dhps A581G has been associated with reduced IPTp-SP efficacy and enhanced morbidity in SP recipients.

Methods

We measured SP-resistance allele frequencies in Malawian women participating in a trial comparing IPTp with SP against intermittent screening by rapid diagnostic tests (ISTp). We genotyped polymerase chain reaction-detected parasites using deep sequencing of SP-resistance alleles.

Results

Among 125 placental infections, A581G-bearing parasites were associated with reduced birth weight (mean difference [MD], 252 g; 95% confidence interval [CI], 46–457; P = .017). Relative to ISTp, IPTp-SP was associated with higher birth weights in women with wild-type parasites (MD, 116 g; 95% CI, −40 to 272; P = .142) and lower birth weights in women with A581G-bearing parasites (MD, 192 g; 95% CI, −264 to 648; P = .385) (Pinteraction = .033). Similar associations were noted on gestational age (Pinteraction = .075). Amongst only IPTp-SP recipients, relative to women who last received SP > 4 weeks before delivery, recent SP receipt was associated with lower birth weight in women with wild-type parasites (MD, 118 g; 95% CI, −376 to 139; P = .361) and higher birth weight in women with A581G-bearing parasites (MD, 783 g; 95% CI, −20 to 1586; P = .054) (Pinteraction = .005).

Conclusions

The effectiveness in birth weight of IPTp-SP is compromised by A581G-bearing parasites, but there was no evidence that the adverse effects of these parasites are exacerbated by antenatal SP.

ISRCTN Registry

www.isrctn.com/ISRCTN69800930.
Diversity of energy metabolism in immune responses regulated by micro-organisms and dietary nutrition
Authors: Hosomi Koji , Kunisawa Jun
Published: March 2020

Immune metabolism has been recognized as a new paradigm in the regulation of host immunity. In the environment, there are many micro-organisms including pathogenic and non-pathogenic and/or beneficial ones. Immune cells exhibit various responses against different types of microbes, which seem to be associated with changes in energy metabolism. In addition, dietary nutrition influences host metabolism and consequent responses by immune cells. In this review, we describe the complex network of immune metabolism from the perspectives of nutrition, micro-organisms and host immunity for the control of immunologic health and diseases.
In vitro activity of omadacycline and levofloxacin against Escherichia coli, Klebsiella pneumoniae and Staphylococcus saprophyticus in human urine supplemented with calcium and magnesium
Authors: Pagano Paul , Marra Andrea , Shinabarger Dean , Pillar Chris
Published: March 2020

Background

Omadacycline, an aminomethylcycline, was approved in 2018 for the treatment of acute bacterial skin and skin structure infections and community-acquired bacterial pneumonia. In a Phase Ib study, around 34% of the absorbed dose of omadacycline was shown to be excreted in urine—an important property for urinary tract infection (UTI) treatment. Therefore, omadacycline has been studied in two Phase II trials for the treatment of uncomplicated UTIs and acute pyelonephritis. The activity of omadacycline against UTI pathogens in human urine is important to understand in this context.

Objectives

To study the in vitro activity of omadacycline against UTI pathogens in human urine supplemented with calcium and magnesium.

Methods

Omadacycline activity was compared with that of levofloxacin against the urinary pathogens Escherichia coli, Klebsiella pneumoniae and Staphylococcus saprophyticus in standard medium, pooled normal human urine and neutral pH-adjusted pooled normal human urine spiked with calcium or magnesium at concentrations consistent with hypercalcaemia and hypermagnesaemia.

Results

The activities of omadacycline and levofloxacin against these urinary pathogens were lower in urine relative to standard medium; addition of Mg2+ to broth and urine had a further negative impact on omadacycline activity, whereas the addition of Ca2+ had less of an impact. Levofloxacin activity was not substantially reduced in either broth or urine by the addition of divalent cations.

Conclusions

The activity of omadacycline against UTI organisms was lower in urine relative to standard medium and was negatively impacted by magnesium. Omadacycline displayed slightly reduced activity when excess calcium was present, but, overall, the differences were ≤2-fold. These observations should be considered along with the pharmacokinetics of the agent for clinical context.
Combination therapy with rifampicin or fosfomycin in patients with Staphylococcus aureus bloodstream infection at high risk for complications or relapse: results of a large prospective observational cohort
Authors: Rieg Siegbert , Ernst Angela , Peyerl-Hoffmann Gabriele , Joost Insa , Camp Johannes , Hellmich Martin , Kern Winfried V , Kaasch Achim J , Seifert Harald
Published: March 2020

Objectives

To investigate whether Staphylococcus aureus bloodstream infection (SAB) patients at high risk for complications or relapse benefit from combination therapy with adjunctive rifampicin or fosfomycin.

Methods

In this post hoc analysis, SAB patients with native valve infective endocarditis, osteoarticular infections or implanted foreign devices were included. The co-primary endpoints were all-cause 90 day mortality and death or SAB-related late complications within 180 days. To overcome treatment selection bias and account for its time dependence, inverse probability of treatment weights were calculated and included in marginal structural Cox proportional hazard models (MSCMs).

Results

A total of 578 patients were included in the analysis, of which 313 (54%) received combination therapy with either rifampicin (n = 242) or fosfomycin (n = 58). In the multivariable MSCM, combination therapy was associated with a better outcome, that is, a lower rate of death or SAB-related late complications within 180 days (HR 0.65, 95% CI 0.46–0.92). This beneficial effect was primarily seen in patients with implanted foreign devices, in which combination therapy was associated with a lower rate of death or SAB-related late complications within 180 days (HR 0.53, 95% CI 0.35–0.79) and a lower 90 day mortality (HR 0.57, 95% CI 0.36–0.91). Upon agent-specific stratification, we found no significant differences in outcomes between combination therapy containing rifampicin and fosfomycin; however, the number of patients in most subgroups was not large enough to draw firm conclusions.

Conclusions

In patients with implanted foreign devices, combination therapy was associated with a better long-term outcome. Larger prospective studies are needed to validate these findings.
In vivo activity of human-simulated regimens of imipenem alone and in combination with relebactam against Pseudomonas aeruginosa in the murine thigh infection model
Authors: Reyes Sergio , Abdelraouf Kamilia , Nicolau David P
Published: March 2020

Background

Imipenem/relebactam is a carbapenem/β-lactamase inhibitor combination with in vitro activity against Pseudomonas aeruginosa and Enterobacterales, including KPC producers.

Objectives

To provide translational data to support the clinical utility of the imipenem/relebactam 500/250 mg q6h regimen using a human-simulated regimen (HSR) of imipenem/relebactam, compared with imipenem alone, against a phenotypically and genotypically diverse population of P. aeruginosa.

Methods

Twenty-nine P. aeruginosa isolates, including KPC (n = 6), PDC (n = 9), PAO (n = 4), GES (n = 5) and VIM (n = 1) producers, were used for the in vivo efficacy studies. Neutropenic mice were thigh-inoculated and randomized to receive HSRs of either imipenem 500 mg q6h, imipenem 1 g q8h, imipenem/relebactam 500/250 mg q6h or saline.

Results

Twenty-seven of the 29 isolates examined were imipenem resistant, with 24/29 isolates showing imipenem MICs of ≥32 mg/L. The addition of relebactam decreased the MICs up to 64-fold; imipenem/relebactam MICs ranged from 0.25 to >32 mg/L. Efficacies of the imipenem monotherapies and the imipenem/relebactam therapy were comparable for the two imipenem-susceptible organisms. Among the imipenem-resistant isolates, an increased mean growth was observed in the imipenem 500 mg q6h HSR and 1 g q8h HSR treatment groups of 1.31 ± 1.01 and 0.18 ± 1.67 log10 cfu/thigh, respectively. In contrast, a ≥2 log reduction in bacterial density was observed in 27/29 (93%) of the imipenem-resistant isolates subjected to imipenem/relebactam 500/250 mg q6h HSR.

Conclusions

The imipenem/relebactam 500/250 mg q6h HSR demonstrated superior in vivo activity compared with the conventionally employed imipenem regimens against MDR P. aeruginosa over a wide range of imipenem/relebactam MICs.
Chromium propionate increases insulin sensitivity in horses following oral and intravenous carbohydrate administration
Authors: Spears Jerry W , Lloyd Karen E , Siciliano Paul , Pratt-Phillips Shannon , Goertzen Ellen W , McLeod Sarah J , Moore Jennifer , Krafka Kristi , Hyda Jill , Rounds Whitney
Published: March 2020

Forty-eight Quarter Horse geldings (3 to 8 yr of age) were used to determine the effects of dietary chromium (Cr), in the form of Cr propionate (Cr Prop) on insulin sensitivity. Horses were blocked by age, body condition score, and glucose response to concentrate feeding on day 0 and randomly assigned to treatments. Treatments consisted of 0, 2, 4, or 8 mg Cr/d from Cr Prop. Horses were fed daily a concentrate mix at a rate of 0.2 kg/100 kg body weight (BW) and grass hay at 1.75 to 2.0 kg/100 kg BW. All horses were fed the control diet for 7 d prior to the initiation of the study. After an overnight fast, blood samples from the jugular vein were obtained at 0, 2, and 4 h after concentrate feeding on days 0 and 28 for the determination of glucose, nonesterified fatty acids, and insulin. A glucose tolerance test (GTT) was conducted on day 42. Glucose was infused via jugular vein catheters, and blood samples were collected at various times relative to dosing for glucose and insulin determination. Plasma glucose on day 28 was affected (P < 0.05) by treatment, time, and treatment × time. Horses fed 4 mg Cr/d had lesser (P < 0.05) plasma glucose concentrations than those in the other treatments at 0 h. At 2 h post-feeding glucose concentrations were greater (P < 0.05) in horses fed 0 or 8 mg Cr/d than in those given 4 mg Cr. Horses fed 2 mg Cr/d had lesser (P < 0.05) plasma glucose at 4 h post feeding compared with those fed 0 or 8 mg Cr. Plasma glucose did not differ among horses receiving 2 or 4 mg Cr/d at 2 or 4 h. Serum insulin was affected (P < 0.05) by treatment, time, and treatment × time. Insulin concentrations were greater (P < 0.05) in horses fed 0 or 2 mg Cr/d than in those given 4 or 8 mg Cr at 0 h. At 4 h post-feeding insulin concentrations were greater (P < 0.05) in horses given 0 or 8 mg Cr than in those fed 2 or 4 mg Cr/d. Plasma glucose was affected (P < 0.05) by treatment and time, but not by treatment × time following the GTT. Mean plasma glucose (across sampling times) concentrations were greater (P < 0.05) in controls than in horses fed 2 or 4 mg Cr/d. Glucose concentrations following the GTT did not differ among controls and horses given 8 mg Cr/d. Following glucose infusion, serum insulin concentrations were greater (P < 0.05) in horses fed 2 or 4 mg Cr and tended to be greater in those fed 8 mg Cr/d compared with controls. The results of this study indicate that 2 or 4 mg Cr/d from Cr Prop increased insulin sensitivity in adult horses following oral carbohydrate consumption.
Development and Validation of an ICP-MS Method and Its Application to Determine Multiple Trace Elements in Small Volumes of Whole Blood and Plasma
Authors: Tanvir E M , Whitfield Karen M , Ng Jack C , Shaw P Nicholas
Published: March 2020

Essential and nonessential element concentrations in human blood provide important information on the nutritional status of individuals and can assist in the screening or diagnosis of certain disorders and their association with other causative factors. A simple and sensitive method, suitable for use with small sample volumes, for quantification of multiple trace element concentrations in whole blood and plasma has been developed using inductively coupled plasma-mass spectrometry. Method validation was performed using standard reference materials of whole blood and serum using varying sample treatments with nitric acid, water and hydrogen peroxide. The method was applied to quantify the trace element concentrations in whole blood and plasma samples (0.1 mL) from 50 adult blood donors in Queensland. The whole blood sample (5 mL) was collected in Vacutainer tubes with K2EDTA as anticoagulant. The developed method was able to quantify, in blood and plasma samples over a wide range of concentrations, several essential elements: cobalt, copper, zinc, iron, manganese and selenium; the nutritionally probably essential elements vanadium and strontium; and nonessential elements including lead, cadmium, arsenic, caesium, barium, thallium and uranium. Significant differences (P < 0.0001) were observed between whole blood and plasma concentrations for 13 elements; 5 of the measured elements, cobalt (0.49 vs. 0.36 μg/L), copper (1.0 vs. 0.75 mg/L), strontium (28 vs. 16 μg/L), barium (1.5 vs. 0.64 μg/L) and thallium (0.06 vs. 0.03 μg/L), had higher mean concentrations in plasma than in blood. Whole blood concentrations of nine trace elements were significantly correlated (P < 0.0001) with plasma concentrations. The distribution of the trace elements between human blood and plasma varied considerably for the different elements. These results indicate that, using a small sample volume, this assay is suitable for the evaluation of nutritional status as well as in monitoring human toxic elemental exposures.
Density-based shape optimization for fail-safe design
Authors: Ambrozkiewicz Olaf , Kriegesmann Benedikt
Published: March 2020

This paper presents a two-stage procedure for density-based optimization towards a fail-safe design. Existing approaches either are computationally extremely expensive or do not explicitly consider fail-safe requirements in the optimization. The current approach trades off both aspects by employing two sequential optimizations to deliver redundant designs that offer robustness to partial failure. In the first stage, a common topology optimization or a topology optimization with local volume constraints is performed. The second stage is referred to as “density-based shape optimization” since it only alters the outline of the structure while still acting on a fixed voxel-type finite element mesh with pseudo-densities assigned to each element. The performance gain and computational efficiency of the current approach are demonstrated by application to various 2D and 3D examples. The results show that, in contrast to explicitly enforcing fail-safety in topology optimization, the current approach can be carried out with reasonable computational cost. Compared to the local volume constraint approach, the suggested procedure further increases the fail-safe performance by 47% for the example considered.
Re-evaluation of the nor mutation and the role of the NAC-NOR transcription factor in tomato fruit ripening
Authors: Gao Ying , Wei Wei , Fan Zhongqi , Zhao Xiaodan , Zhang Yiping , Jing Yuan , Zhu Benzhong , Zhu Hongliang , Shan Wei , Chen Jianye , Grierson Donald , Luo Yunbo , Jemrić Tomislav , Jiang Cai-Zhong , Fu Da-Qi
Published: March 2020

The tomato non-ripening (nor) mutant generates a truncated 186-amino-acid protein (NOR186) and has been demonstrated previously to be a gain-of-function mutant. Here, we provide more evidence to support this view and answer the open question of whether the NAC-NOR gene is important in fruit ripening. Overexpression of NAC-NOR in the nor mutant did not restore the full ripening phenotype. Further analysis showed that the truncated NOR186 protein is located in the nucleus and binds to but does not activate the promoters of 1-aminocyclopropane-1-carboxylic acid synthase2 (SlACS2), geranylgeranyl diphosphate synthase2 (SlGgpps2), and pectate lyase (SlPL), which are involved in ethylene biosynthesis, carotenoid accumulation, and fruit softening, respectively. The activation of the promoters by the wild-type NOR protein can be inhibited by the mutant NOR186 protein. On the other hand, ethylene synthesis, carotenoid accumulation, and fruit softening were significantly inhibited in CR-NOR (CRISPR/Cas9-edited NAC-NOR) fruit compared with the wild-type, but much less severely affected than in the nor mutant, while they were accelerated in OE-NOR (overexpressed NAC-NOR) fruit. These data further indicated that nor is a gain-of-function mutation and NAC-NOR plays a significant role in ripening of wild-type fruit.
Chloride is beneficial for growth of the xerophyte Pugionium cornutum by enhancing osmotic adjustment capacity under salt and drought stresses
Authors: Cui Yan-Nong , Li Xiao-Ting , Yuan Jian-Zhen , Wang Fang-Zhen , Guo Huan , Xia Zeng-Run , Wang Suo-Min , Ma Qing
Published: March 2020

Chloride (Cl–) is pervasive in saline soils, and research on its influence on plants has mainly focused on its role as an essential nutrient and its toxicity when excessive accumulation occurs. However, the possible functions of Cl– in plants adapting to abiotic stresses have not been well documented. Previous studies have shown that the salt tolerance of the xerophytic species Pugionium cornutum might be related to high Cl– accumulation. In this study, we investigated the Cl–-tolerant characteristics and possible physiological functions of Cl– in the salt tolerance and drought resistance of P. cornutum. We found that P. cornutum can accumulate a large amount of Cl– in its shoots, facilitating osmotic adjustment and turgor generation under saline conditions. Application of DIDS (4,4´-diisothiocyanostilbene-2,2´-disulfonic acid), a blocker of anion channels, significantly inhibited Cl– uptake, and decreased both the Cl– content and its contribution to leaf osmotic adjustment, resulting in the exacerbation of growth inhibition in response to NaCl. Unlike glycophytes, P. cornutum was able to maintain NO3– homeostasis in its shoots when large amounts of Cl– were absorbed and accumulated. The addition of NaCl mitigated the deleterious effects of osmotic stress on P. cornutum because Cl– accumulation elicited a strong osmotic adjustment capacity. These findings suggest that P. cornutum is a Cl–-tolerant species that can absorb and accumulate Cl– to improve growth under salt and drought stresses.
Jasmonate-independent regulation of digestive enzyme activity in the carnivorous butterwort Pinguicula × Tina
Authors: Kocáb Ondřej , Jakšová Jana , Novák Ondřej , Petřík Ivan , Lenobel René , Chamrád Ivo , Pavlovič Andrej
Published: March 2020

Carnivorous plants within the order Caryophyllales use jasmonates, a class of phytohormone, in the regulation of digestive enzyme activities. We used the carnivorous butterwort Pinguicula × Tina from the order Lamiales to investigate whether jasmonate signaling is a universal and ubiquitous signaling pathway that exists outside the order Caryophyllales. We measured the electrical signals, enzyme activities, and phytohormone tissue levels in response to prey capture. Mass spectrometry was used to identify proteins in the digestive secretion. We identified eight enzymes in the digestive secretion, many of which were previously found in other genera of carnivorous plants. Among them, alpha-amylase is unique in carnivorous plants. Enzymatic activities increased in response to prey capture; however, the tissue content of jasmonic acid and its isoleucine conjugate remained rather low in contrast to the jasmonate response to wounding. Enzyme activities did not increase in response to the exogenous application of jasmonic acid or coronatine. Whereas similar digestive enzymes were co-opted from plant defense mechanisms among carnivorous plants, the mode of their regulation differs. The butterwort has not co-opted jasmonate signaling for the induction of enzyme activities in response to prey capture. Moreover, the presence of alpha-amylase in digestive fluid of P. × Tina, which has not been found in other genera of carnivorous plants, might indicate that non-defense-related genes have also been co-opted for carnivory.
The isoleucic acid triad: distinct impacts on plant defense, root growth, and formation of reactive oxygen species
Authors: Bauer Sibylle , Mekonnen Dereje W , Geist Birgit , Lange Birgit , Ghirardo Andrea , Zhang Wei , Schäffner Anton R
Published: March 2020

Isoleucic acid (ILA), a branched-chain amino acid-related 2-hydroxycarboxylic acid, occurs ubiquitously in plants. It enhances pathogen resistance and inhibits root growth of Arabidopsis. The salicylic acid (SA) glucosyltransferase UGT76B1 is able to conjugate ILA. Here, we investigate the role of ILA in planta in Arabidopsis and reveal a triad of distinct responses to this small molecule. ILA synergistically co-operates with SA to activate SA-responsive gene expression and resistance in a UGT76B1-dependent manner in agreement with the observed competitive ILA-dependent repression of SA glucosylation by UGT76B1. However, ILA also shows an SA-independent stress response. Nitroblue tetrazolium staining and pharmacological experiments indicate that ILA induces superoxide formation of the wild type and of an SA-deficient (NahG sid2) line. In contrast, the inhibitory effect of ILA on root growth is independent of both SA and superoxide induction. These effects of ILA are specific and distinct from its isomeric compound leucic acid and from the amino acid isoleucine. Leucic acid and isoleucine do not induce expression of defense marker genes or superoxide production, whereas both compounds inhibit root growth. All three responses to ILA are also observed in Brassica napus.
Interconnecting music repositories with semantic web technologies—An RDF- and schema.org-based approach
Authors: Neovesky Anna , von Vlahovits Frederic
Published: March 2020

The growing amount of openly available research data enables various possibilities of reuse. Data can be analyzed, visualized, or even further processed, enriched, and combined with other sources to enable new research questions and a different view on the material. Several catalogues and research tools aggregate collections on specific topics to make them searchable and reusable. To be able to bring together different collections, a common data standard is necessary. This article discusses how semantic web technologies can be used to connect digital as well as analogue music catalogues and music editions. The article first discusses how music can be searched using its characteristic melody and presents the approach of the open-source search engine for music incipits, IncipitSearch. Subsequently, the advantages of semantic web standards for musicology are highlighted. Then, the underlying RDF- and schema.org-based metadata standard, which is used to aggregate and distribute the data, is discussed. The article concludes with an outlook on research perspectives for digital musicology and musicology in general.
The average physical properties of A-G stars derived from uvby-H β Strömgren–Crawford photometry as the basis for a spectral-classification synthetical approach
Authors: Dalle Mese G , López-Cruz O , Schuster W J , Chavarría-K C , Ibarra-Medel H J
Published: March 2020

We have revisited and updated the uvby Strömgren colour and colour–index distributions of A-, F-, and early G-type main-sequence stars. For this aim, we selected 7054 dwarf stars along with 65 Morgan–Keeman (MK) standard stars within the same spectral range but covering all luminosity classes. The standard stars were selected following the MK mandate strictly, using spectra taken at classification resolution recorded on photographic plates. We used the colours of these stars to determine the effective temperature and surface gravity. After correcting for systematic offsets using fundamental parameters and considering a few exceptions, we find a one-to-one correspondence, among MK spectral types, Strömgren photometry, and their associated physical properties.

We have applied a principal component analysis to the mean Strömgren indices for dwarf stars complemented by MK standards for higher luminosity classes. We have used the projections to introduce three new photometric metaindices, namely SM1, SM2, and SM3. We have defined a 3D-box, which allowed us to segregate dwarf stars from bright giants and supergiant stars, with the aid of the metaindices. Two of the planes show that the projections of dwarfs and supergiants are ordered by temperature; however, the temperature dependence for the supergiants is not as well defined as for the dwarfs. Following the MK Process, we were able to form an automatic classifier. We present some applications and assigned synthetical spectral types. We suggest that our metaindices formalism allows the extension of Strömgren photometric outside its original mandate (i.e. later types), without requiring the introduction of additional photometric filters.
Constraining radio mode feedback in galaxy clusters with the cluster radio AGNs properties to z ∼ 1
Authors: Gupta N , Pannella M , Mohr J J , Klein M , Rykoff E S , Annis J , Avila S , Bianchini F , Brooks D , Buckley-Geer E , Bulbul E , Carnero Rosell A , Carrasco Kind M , Carretero J , Chiu I , Costanzi M , da Costa L N , De Vicente J , Desai S , Dietrich J P , Doel P , Everett S , Evrard A E , García-Bellido J , Gaztanaga E , Gruen D , Gruendl R A , Gschwend J , Gutierrez G , Hollowood D L , Honscheid K , James D J , Jeltema T , Kuehn K , Lidman C , Lima M , Maia M A G , Marshall J L , McDonald M , Menanteau F , Miquel R , Ogando R L C , Palmese A , Paz-Chinchón F , Plazas A A , Reichardt C L , Sanchez E , Santiago B , Saro A , Scarpine V , Schindler R , Schubnell M , Serrano S , Sevilla-Noarbe I , Shao X , Smith M , Stott J P , Strazzullo V , Suchyta E , Swanson M E C , Vikram V , Zenteno A
Published: March 2020

We study the properties of the Sydney University Molonglo Sky Survey (SUMSS) 843 MHz radio active galactic nuclei (AGNs) population in galaxy clusters from two large catalogues created using the Dark Energy Survey (DES): ∼11 800 optically selected RM-Y3 and ∼1000 X-ray selected MARD-Y3 clusters. We show that cluster radio loud AGNs are highly concentrated around cluster centres to $z$ ∼ 1. We measure the halo occupation number for cluster radio AGNs above a threshold luminosity, finding that the number of radio AGNs per cluster increases with cluster halo mass as N ∝ M1.2 ± 0.1 (N ∝ M0.68 ± 0.34) for the RM-Y3 (MARD-Y3) sample. Together, these results indicate that radio mode feedback is favoured in more massive galaxy clusters. Using optical counterparts for these sources, we demonstrate weak redshift evolution in the host broad-band colours and the radio luminosity at fixed host galaxy stellar mass. We use the redshift evolution in radio luminosity to break the degeneracy between density and luminosity evolution scenarios in the redshift trend of the radio AGNs luminosity function (LF). The LF exhibits a redshift trend of the form (1 + $z$)γ in density and luminosity, respectively, of γD = 3.0 ± 0.4 and γP = 0.21 ± 0.15 in the RM-Y3 sample, and γD = 2.6 ± 0.7 and γP = 0.31 ± 0.15 in MARD-Y3. We discuss the physical drivers of radio mode feedback in cluster AGNs, and we use the cluster radio galaxy LF to estimate the average radio-mode feedback energy as a function of cluster mass and redshift and compare it to the core (<0.1R500) X-ray radiative losses for clusters at $z$ < 1.
Microlensing of radially pulsating stars
Authors: Sajadian Sedighe , Ignace Richard
Published: March 2020

In this paper, we study the microlensing of radially pulsating stars. It is possible to discern and characterize the properties of distant, faint pulsating stars using high-cadence microlensing observations. By combining the stellar variability period with microlensing, we can obtain the source distance, type and radius, and we can better determine the lens parameters. Considering the variations in the radius and surface temperature of radially pulsating stars periodically, their microlensing light curves can be obtained by multiplying the magnification factor with a variable finite size effect by the intrinsic brightness curves of the pulsing source. The variable finite size of the source due to pulsation can be significant for transit and single microlensing with caustic-crossing features. This type of deviation in the magnification factor is considerable when the ratio of the source radius to the projected lens–source distance is in the range of ρ⋆/u ∈ [0.4, 10] and when its duration is short and of the same order as the time of crossing the source radius. Other deviations due to variable source intensity and its area make coloured and periodic deviations, which are asymmetric with respect to the signs of the pulsation phase. The positive phase makes deviations with larger amplitude than the negative phase. These deviations dominate in filters with short wavelengths (e.g. the B band). The position of the magnification peaks in the microlensing of variable stars varies and this displacement differs in different filters.
The role of wind driving in OB star bow nebulae
Authors: Struck Curtis
Published: March 2020

Bow-shaped mid-infrared (mid-IR) emission regions have been discovered in satellite observations of numerous late-type O and early-type B stars with moderate velocities relative to the ambient interstellar medium. Previously, hydrodynamical bow shock models have been used to study this emission. It appears that such models are incomplete in that they neglect kinetic effects associated with long mean free paths of stellar wind particles, and the complexity of Weibel instability fronts. Wind ions are scattered in the Weibel instability and mix with the interstellar gas. However, they do not lose their momentum and most ultimately diffuse further into the ambient gas like cosmic rays, and share their energy and momentum. Lacking other coolants, the heated gas transfers energy into interstellar dust grains, which radiate it. This process, in addition to grain photoheating, provides the energy for the emission. A weak R-type ionization front, formed well outside the IR emission region, generally moderates the interstellar gas flow into the emission region. The theory suggests that the IR emission process is limited to cases of moderate stellar peculiar velocities, evidently in accord with the observations.
On rising magnetic flux tube and formation of sunspots in a deep domain
Authors: Hotta H , Iijima H
Published: March 2020

We investigate the rising flux tube and the formation of sunspots in an unprecedentedly deep computational domain that covers the whole convection zone with a radiative magnetohydrodynamics simulation. Previous calculations had shallow computational boxes (<30 Mm) and convection zones at a depth of 200 Mm. By using our new numerical code Radition and RSST for Deep Dynamics(r2d2), we succeed in covering the whole convection zone and reproduce the formation of the sunspot from a simple horizontal flux tube because of the turbulent thermal convection. The main findings are as follows. (1) The rising speed of the flux tube is larger than the upward convection velocity because of the low density caused by the magnetic pressure and the suppression of the mixing. (2) The rising speed of the flux tube exceeds 250 m s−1 at a depth of 18 Mm, while we do not see any clear evidence of the divergent flow 3 h before the emergence at the solar surface. (3) Initially, the root of the flux tube is filled with the downflows, and then the upflow fills the centre of the flux tube during the formation of the sunspot. (4) The essential mechanisms for the formation of the sunspot are the coherent inflow and the turbulent transport. (5) The low-temperature region is extended to a depth of at least 40 Mm in the matured sunspot, with the high-temperature region in the centre of the flux tube. Some of the findings indicate the importance of the deep computational domain for the flux emergence simulations.
Stability analysis of three exoplanet systems
Authors: Marshall J P , Horner J , Wittenmyer R A , Clark J T , Mengel M W
Published: March 2020

The orbital solutions of published multiplanet systems are not necessarily dynamically stable on time-scales comparable to the lifetime of the system as a whole. For this reason, dynamical tests of the architectures of proposed exoplanetary systems are a critical tool to probe the stability and feasibility of the candidate planetary systems, with the potential to point the way towards refined orbital parameters of those planets. Such studies can even help in the identification of additional companions in such systems. Here, we examine the dynamical stability of three planetary systems, orbiting HD 67087, HD 110014, and HD 133131A. We use the published radial velocity measurements of the target stars to determine the best-fitting orbital solutions for these planetary systems using the systemic console. We then employ the N-body integrator mercury to test the stability of a range of orbital solutions lying within 3σ of the nominal best fit for a duration of 100 Myr. From the results of the N-body integrations, we infer the best-fitting orbital parameters using the Bayesian package astroemperor. We find that both HD 110014 and HD 133131A have long-term stable architectures that lie within the 1σ uncertainties of the nominal best fit to their previously determined orbital solutions. However, the HD 67087 system exhibits a strong tendency towards instability on short time-scales. We compare these results to the predictions made from consideration of the angular momentum deficit criterion, and find that its predictions are consistent with our findings.
Cluster membership for the long-period Cepheid calibrator SV Vul
Authors: Negueruela I , Dorda R , Marco A
Published: March 2020

Classical Cepheids represent the first step of the distance scale ladder. Claims of tension between the locally calculated Hubble constant and the values deduced from Planck’s results have sparked new interest in these distance calibrators. Cluster membership provides an independent distance measurement, as well as astrophysical context for studies of their stellar properties. Here, we report the discovery of a young open cluster in the vicinity of SV Vul, one of the most luminous Cepheids known in the Milky Way. Gaia DR2 data show that SV Vul is a clear astrometric and photometric member of the new cluster, which we name Alicante 13. Although dispersed, Alicante 13 is moderately well populated, and contains three other luminous stars, one early-A bright giant and two low-luminosity red supergiants. The cluster is about 30 Ma old at a nominal distance of 2.5 kpc. With this age, SV Vul should have a mass around $10$ M⊙, in good accordance with its luminosity, close to the highest luminosity for Cepheids allowed by recent stellar models.