bluuebunny/crossref_metadata_2025 huggingface dataset:
Cell-free DNA methylation reveals cell-specific tissue injury and correlates with disease severity and patient outcomes in COVID-19
Authors: Li Yuan-Yuan , Yuan Ming-Ming , Li Yuan-Yuan , Li Shan , Wang Jing-Dong , Wang Yu-Fei , Li Qian , Li Jun , Chen Rong-Rong , Peng Jin-Min , Du Bin
Published: March 2024

Background

The recently identified methylation patterns specific to cell type allows the tracing of cell death dynamics at the cellular level in health and diseases. This study used COVID-19 as a disease model to investigate the efficacy of cell-specific cell-free DNA (cfDNA) methylation markers in reflecting or predicting disease severity or outcome.

Methods

Whole genome methylation sequencing of cfDNA was performed for 20 healthy individuals, 20 cases with non-hospitalized COVID-19 and 12 cases with severe COVID-19 admitted to intensive care unit (ICU). Differentially methylated regions (DMRs) and gene ontology pathway enrichment analyses were performed to explore the locus-specific methylation difference between cohorts. The proportion of cfDNA derived from lung and immune cells to a given sample (i.e. tissue fraction) at cell-type resolution was estimated using a novel algorithm, which reflects lung injuries and immune response in COVID-19 patients and was further used to evaluate clinical severity and patient outcome.

Results

COVID‑19 patients had globally reduced cfDNA methylation level compared with healthy controls. Compared with non-hospitalized COVID-19 patients, the cfDNA methylation pattern was significantly altered in severe patients with the identification of 11,156 DMRs, which were mainly enriched in pathways related to immune response. Markedly elevated levels of cfDNA derived from lung and more specifically alveolar epithelial cells, bronchial epithelial cells, and lung endothelial cells were observed in COVID-19 patients compared with healthy controls. Compared with non-hospitalized patients or healthy controls, severe COVID-19 had significantly higher cfDNA derived from B cells, T cells and granulocytes and lower cfDNA from natural killer cells. Moreover, cfDNA derived from alveolar epithelial cells had the optimal performance to differentiate COVID-19 with different severities, lung injury levels, SOFA scores and in-hospital deaths, with the area under the receiver operating characteristic curve of 0.958, 0.941, 0.919 and 0.955, respectively.

Conclusion

Severe COVID-19 has a distinct cfDNA methylation signature compared with non-hospitalized COVID-19 and healthy controls. Cell type-specific cfDNA methylation signature enables the tracing of COVID-19 related cell deaths in lung and immune cells at cell-type resolution, which is correlated with clinical severities and outcomes, and has extensive application prospects to evaluate tissue injuries in diseases with multi-organ dysfunction.
Pannorin isolated from marine Penicillium sp. SG-W3: a selective monoamine oxidase A inhibitor
Authors: Oh Jong Min , Gao Qian , Shin Woong-Hee , Lee Eun-Young , Chung Dawoon , Choi Grace , Nam Sang-Jip , Kim Hoon
Published: March 2024

Six compounds were isolated from Penicillium sp. SG-W3, a marine-derived fungus, and their inhibitory activities against target enzymes relating to neurological diseases were evaluated. Compound 1 (pannorin) was a potent and selective monoamine oxidase (MAO)-A inhibitor with a 50% inhibitory concentration (IC50) of 1.734 μM and a selectivity index (SI) of > 23.07 versus MAO-B, and it showed an efficient antioxidant activity. All compounds showed weak inhibitory activities against acetylcholinesterase, butyrylcholinesterase, and β-secretase. The inhibition constant (Ki) of 1 for MAO-A was 1.049 ± 0.030 μM with competitive inhibition. Molecular docking simulation predicted that compound 1 forms hydrogen bonds with MAO-A, and binds more tightly to MAO-A than to MAO-B (− 25.02 and − 24.06 kcal/mol, respectively). These results suggest that compound 1 is a selective, reversible, and competitive MAO-A inhibitor that can be a therapeutic candidate for treating neurological diseases.
Changes of Mycobacterium tuberculosis specific antigen-stimulated CD27−CD38+IFN-γ+CD4+ T cells before and after anti-tuberculosis treatment
Authors: Fang Yong , Tang Yuan , Luo Qiao-Xia , Wang Na , Tang Liang , Yang Xiao-Jun , You Xiao-Fang , Wang Yu-Chun , Liang Li , Zhang Jing-Bo , Su Bo , Sha Wei
Published: March 2024

Background

The aim of the study was to investigate whether the expression of CD27−CD38+ in interferon (IFN)-γ+CD4+ T cells stimulated by the specific antigen early secreted antigenic target-6 (ESAT-6)/culture filter protein-10 (CFP-10) could be a potential new therapeutic evaluation indicator for anti-tuberculosis (TB) treatment.

Methods

Newly diagnosed active pulmonary TB patients, latent TB infection (LTBI) and healthy controls were enrolled from January 2021 to December 2021. PTB patients were treated by standard anti-TB regimen 2HREZ/4HR (2 months of isoniazid (H), rifampin (R), ethambutol (E), and pyrazinamide (Z) followed by 4 months of isoniazid (H) and rifampin (R)). The difference of CD27−CD38+ expression in IFN-γ+CD4+ T cells before treatment, 2 months after treatment, and 6 months after treatment were compared.

Results

Total 45 PTB patients, 38 LTBI cases and 43 healthy controls were enrolled. The expression of CD27−CD38+ decreased significantly after anti-TB treatment and was comparable with that in LTBI and healthy controls when the 6-month anti-TB treatment course was completed. The decline rate of CD27−CD38+ between 6 months after treatment and baseline was positively correlated with erythrocyte sedimentation rate (r = 0.766, P < 0.0001), C-reactive protein (r = 0.560, P = 0.003) and chest computerized tomography severity score (r = 0.632, P = 0.0005). The area under receiver operator characteristic curve of CD27−CD38+ in distinguish pulmonary TB patients before and after treatment was 0.779.

Conclusion

The expression of CD27−CD38+ in ESAT-6/CFP-10 stimulated IFN-γ+CD4+T cells can well reflect the changes of the disease before and after anti-TB treatment, which is expected to be a potential new therapeutic evaluation index.

Clinical Registry number chiCTR1800019966.
TR4 worsen urosepsis by regulating GSDMD
Authors: Wang Huan , Zhu Shibin , Zhou Zhenwei , Wang Zhenghui , Zhuang Wei , Xue Dingwei , Lu Zeyi , Zheng Qiming , Ding Lifeng , Ren Liangliang , Luo Wenqing , Wang Ruyue , Ge Guangju , Xia Liqun , Li Gonghui , Wu Haiyang
Published: March 2024

Background

Urosepsis is a life-threatening organ disease in which pathogenic microorganisms in the urine enter the blood through the vessels, causing an imbalance in the immune response to infection. The aim of this study was to elucidate the role of testicular orphan receptor 4 (TR4) in urosepsis.

Methods

The role of TR4 in the progression and prognosis of urosepsis was confirmed by analyzing data from online databases and clinical human samples. To mimic urosepsis, we injected E. coli bacteria into the renal pelvis of mice to create a urosepsis model. Hematoxylin and eosin staining was used to observe histopathological changes in urosepsis. The effects of the upregulation or downregulation of TR4 on macrophage pyroptosis were verified in vitro. Chromatin immunoprecipitation assay was used to verify the effect of TR4 on Gasdermin D (GSDMD) transcription.

Results

TR4 was more highly expressed in the nonsurviving group than in the surviving group. Furthermore, overexpressing TR4 promoted inflammatory cytokine expression, and knocking down TR4 attenuated inflammatory cytokine expression. Mechanistically, TR4 promoted pyroptosis by regulating the expression of GSDMD in urosepsis. Furthermore, we also found that TR4 knockdown protected mice from urosepsis induced by the E. coli.

Conclusions

TR4 functions as a key regulator of urosepsis by mediating pyroptosis, which regulates GSDMD expression. Targeting TR4 may be a potential strategy for urosepsis treatment.
Mutations in TSPAN12 gene causing familial exudative vitreoretinopathy
Authors: Ju Yuqiao , Chen Tianhui , Ruan Lu , Zhao Ye , Chang Qing , Huang Xin
Published: March 2024

Background

To report newly found TSPAN12 mutations with a unique form of familial exudative vitreoretinopathy (FEVR) and find out the possible mechanism of a repeated novel intronic variant in TSPAN12 led to FEVR.

Results

Nine TSPAN12 mutations with a unique form of FEVR were detected by panel-based NGS. MINI-Gene assay showed two splicing modes of mRNA that process two different bands A and B, and mutant-type shows replacement with the splicing mode of Exon11 hopping. Construction of wild-type and mutant TSPAN12 vector showed the appearance of premature termination codons (PTC). In vitro expression detection showed significant down-regulated expression level of TSPAN12 mRNAs and proteins in cells transfected with mutant vectors compared with in wild-type group. On the contrary, translation inhibitor CHX and small interfering RNA of UPF1 (si-UPF1) significantly increased mRNA or protein expression of TSPAN12 in cells transfected with the mutant vectors.

Conclusions

Nine mutations in TSPAN12 gene are reported in 9 FEVR patients with a unique series of ocular abnormalities. The three novel TSPAN12 mutations trigger NMD would cause the decrease of TSPAN12 proteins that participate in biosynthesis and assembly of microfibers, which might lead to FEVR, and suggest that intronic sequence analysis might be a vital tool for genetic counseling and prenatal diagnoses.
“Maze Out”: a study protocol for a randomised controlled trial using a mix methods approach exploring the potential and examining the effectiveness of a serious game in the treatment of eating disorders
Authors: Guala Maria Mercedes , Bikic Aida , Bul Kim , Clinton David , Mejdal Anna , Nielsen Helene Nygaard , Stenager Elsebeth , Søgaard Nielsen Anette
Published: March 2024

Background

Eating Disorders (ED) are severe and costly mental health disorders. The effects of existing treatment approaches are limited and there is a need to develop novel interventions, including digital strategies that can increase engagement and effectiveness. Maze Out is a new serious game coproduced by patients and ED therapists, which allows patients to “play” with the reality of an ED and reflect on associated challenges.

Objectives

The present study has two main objectives: (1) to evaluate the effectiveness of adding Maze Out to treatment as usual (TAU) in a randomised controlled trial (RCT); and (2) to examine in depth the potential of Maze Out by examining how it is perceived and used in the context of an RCT.

Methods

Participants will be recruited from mental health care services, endocrinology departments or Community Centres offering treatment for ED. Patients suffering from ED (N = 94) will be randomised to either TAU or TAU plus Maze Out. Primary outcome will be measured in terms of changes in self-efficacy, measured by a 5-item self-efficacy questionnaire (5-item SE_ED). Secondary outcome measures will include feelings of ineffectiveness and self-image, as measured by Eating Disorder Inventory, version 3 (EDI-3), Brief INSPIRE-O and Structural Analysis of Social Behaviour Intrex Questionnaire (SAS-B). Data will be collected at baseline (enrolment in the study), and subsequently 8 and 15 weeks after inclusion. Experiences of playing Maze Out will be examined in a sub-sample of participants, utilising both quantitative user analytics and qualitative interview data of patients, interview data of significant others, and healthcare professionals to explore the possible impact of Maze Out on disorder insight, communication patterns between patients and therapists and understanding of their disorder.

Discussion

To our knowledge Maze Out is the first serious game coproduced by patients and therapists. It is a novel and theoretically grounded intervention that may significantly contribute to the healing process of ED. If found effective, the potential for wide-spread impact and scalability is considerable.

Trial registration ClinicalTrials.gov NCT05621018.
Body composition is related to cognitive function among young adults in Ghana
Authors: Fabea Linda , Intiful Freda Dzifa , Hatsu Irene E. , Larry-Afutu Joana , Boateng Laurene
Published: March 2024

Background

A growing body of evidence suggests that obesity can affect cognitive function. However, it is unclear whether this effect is independent of obesity-related comorbidities. This study thus sought to determine the association between body composition and cognitive function of young adults in Ghana with less predisposition to obesity-related comorbidities.

Methods

A cross-sectional study design was employed, involving 381 participants recruited by simple random sampling. After consenting, participants completed questionnaires that assessed sociodemographic characteristics, along with assessments for anthropometric measures and cognitive function. Analyses for associations were conducted by either Pearson’s correlation test or chi-Square test of independence.

Results

Over half (60%) of participants were females and 69.6% were in the first year with a mean age of 20.18 ± 2.52 years. Based on Pearson’s correlation test, no significant association was found between Body Mass Index (BMI) and Waist to Hip Ratio (WHR), and Trail Making Test-A and B (TMT-A and TMT-B). Nonetheless, a chi-square test showed a significant association, between BMI and TMT-A (p = 0.01), and WHR and both TMT-A (p = 0.001) and TMT-B (p = 0.02). Weak direct correlations were found between body fat percentage and TMT-A (r = 0.120, p = 0.019) and TMT-B (r = 0.133, p = 0.009). Further, a weak inverse correlation was found between muscle mass and TMT-A (r = − 0.141, p = 0.006) and TMT-B (r = − 0.144, p = 0.005).

Conclusion

High body fat, low muscle mass and body fat distribution may have a significant association with cognitive functions and must be considered in obesity interventions. This study provided more insight on the association between BMI and cognitive function and would be helpful in designing new weight management interventions or modifying existing interventions to consider the influence of obesity on cognitive function.
The relationship between theory of mind and moral sensitivity among Chinese preschool children: the mediating role of empathy
Authors: Du Jun , Liang Yuan , Guo Di , Xiao Ying
Published: March 2024

Background

Identifying moral behavior in complex situations is the key ability for children to develop prosocial behavior. The theory of mind (ToM) and empathy provide the cognition and emotional motivation required for the development of moral sensitivity. In this study, we investigated the associations among ToM, empathy, and moral sensitivity and explored the possible differences between Chinese preschool children aged 4 and 5 years.

Methods

One hundred and thirty children completed the unexpected-content and change-of-location tasks as well as questionnaires about empathy and moral sensitivity individually. A one-way analysis of variance and the multi-group mediation SEM were used to examine the associations of the three variables and age differences.

Results

The scores of 5-year-old children in the dimensions of care, fairness, authority, and sanctity and the total score were higher than those of 4-year-old children. Moral sensitivity was positively correlated with both ToM and empathy after we controlled for verbal IQ and gender. Multigroup mediation analyses showed age-based differences in the associations among moral sensitivity, ToM, and empathy. Empathy’s mediation effect was partial among 4-year-old children and complete among 5-year-old children.

Conclusions

These findings contribute to understanding the cognitive and emotional factors in the formation of children’s moral sensitivity. They also point to a promising approach to promoting the development of moral sensitivity and evidence for educators to understand the process of children’s socialization.
Psychometric evidence of the Acceptance and Action Questionnaire-II (AAQ-II): an item response theory analysis in university students from Chile
Authors: Langer Álvaro I. , Ponce Fernando P. , Ordóñez-Carrasco Jorge L. , Fuentes-Ferrada Reiner , Mac-Ginty Scarlett , Gaete Jorge , Núñez Daniel
Published: March 2024

Background

Experiential avoidance (EA) is a psychological mechanism associated with several mental health disorders and is regarded as a relevant target by third-generation cognitive behavioral therapies. It has been mainly assessed through self-report questionnaires, and the AAQ-II is the most used tool. Its psychometric evidence has been mostly tested through the classical test theory (CTT) and very scarcely assessed through Item Response Theory (IRT).

Methods

We used the Graded Response Model to examine its psychometric properties in Spanish-speaking university students (n = 1503; women = 995 (66.2%), mean age = 19.29, SD = 2.45). We tested whether the empirical data fit the model’s predictions and estimated the dispersion of persons and items along the experiential avoidance continuum. Moreover, we examined category probability curves to identify the response probability of each answer. Likewise, an item-person map was made where the measurement of persons and items, both on the same scale and along the experiential avoidance continuum, could be observed jointly. Finally, we tested the gender invariance of the scale.

Results

We found that the values of the individuals and the items were in the established range to be considered an adequate measure of EA. Additionally, we observed high discrimination indices for all items. The current version with seven answer options could not be optimal and should be tested in future studies. Finally, we found evidence of differential functioning by gender in one of the seven items of the instrument.

Conclusions

Our results indicate that the AAQ-II is a suitable tool for measuring EA and accurately classifying and differentiating EA levels in university students.
Overlapping yet dissociable contributions of superiority illusion features to Ponzo illusion strength and metacognitive performance
Authors: Matsuyoshi Daisuke , Isato Ayako , Yamada Makiko
Published: March 2024

Humans are typically inept at evaluating their abilities and predispositions. People dismiss such a lack of metacognitive insight into their capacities while even enhancing (albeit illusorily) self-evaluation such that they should have more desirable traits than an average peer. This superiority illusion helps maintain a healthy mental state. However, the scope and range of its influence on broader human behavior, especially perceptual tasks, remain elusive. As belief shapes the way people perceive and recognize, the illusory self-superiority belief potentially regulates our perceptual and metacognitive performance. In this study, we used hierarchical Bayesian estimation and machine learning of signal detection theoretic measures to understand how the superiority illusion influences visual perception and metacognition for the Ponzo illusion. Our results demonstrated that the superiority illusion correlated with the Ponzo illusion magnitude and metacognitive performance. Next, we combined principal component analysis and cross-validated regularized regression (relaxed elastic net) to identify which superiority components contributed to the correlations. We revealed that the “extraversion” superiority dimension tapped into the Ponzo illusion magnitude and metacognitive ability. In contrast, the “honesty-humility” and “neuroticism” dimensions only predicted Ponzo illusion magnitude and metacognitive ability, respectively. These results suggest common and distinct influences of superiority features on perceptual sensitivity and metacognition. Our findings contribute to the accumulating body of evidence indicating that the leverage of superiority illusion is far-reaching, even to visual perception.
Bridging the gap between movement data and connectivity analysis using the Time-Explicit Habitat Selection (TEHS) model
Authors: Valle Denis , Attias Nina , Cullen Joshua A. , Hooten Mevin B. , Giroux Aline , Oliveira-Santos Luiz Gustavo R. , Desbiez Arnaud L. J. , Fletcher Robert J.
Published: March 2024

Background

Understanding how to connect habitat remnants to facilitate the movement of species is a critical task in an increasingly fragmented world impacted by human activities. The identification of dispersal routes and corridors through connectivity analysis requires measures of landscape resistance but there has been no consensus on how to calculate resistance from habitat characteristics, potentially leading to very different connectivity outcomes.

Methods

We propose a new model, called the Time-Explicit Habitat Selection (TEHS) model, that can be directly used for connectivity analysis. The TEHS model decomposes the movement process in a principled approach into a time and a selection component, providing complementary information regarding space use by separately assessing the drivers of time to traverse the landscape and the drivers of habitat selection. These models are illustrated using GPS-tracking data from giant anteaters (Myrmecophaga tridactyla) in the Pantanal wetlands of Brazil.

Results

The time model revealed that the fastest movements tended to occur between 8 p.m. and 5 a.m., suggesting a crepuscular/nocturnal behavior. Giant anteaters moved faster over wetlands while moving much slower over forests and savannas, in comparison to grasslands. We also found that wetlands were consistently avoided whereas forest and savannas tended to be selected. Importantly, this model revealed that selection for forest increased with temperature, suggesting that forests may act as important thermal shelters when temperatures are high. Finally, using the spatial absorbing Markov chain framework, we show that the TEHS model results can be used to simulate movement and connectivity within a fragmented landscape, revealing that giant anteaters will often not use the shortest-distance path to the destination patch due to avoidance of certain habitats.

Conclusions

The proposed approach can be used to characterize how landscape features are perceived by individuals through the decomposition of movement patterns into a time and a habitat selection component. Additionally, this framework can help bridge the gap between movement-based models and connectivity analysis, enabling the generation of time-explicit connectivity results.
Prophylactic nicotinamide treatment protects from rotenone-induced neurodegeneration by increasing mitochondrial content and volume
Authors: Otmani Amin , Jóhannesson Gauti , Brautaset Rune , Tribble James R. , Williams Pete A.
Published: March 2024

Leber’s hereditary optic neuropathy (LHON) is driven by mtDNA mutations affecting Complex I presenting as progressive retinal ganglion cell dysfunction usually in the absence of extra-ophthalmic symptoms. There are no long-term neuroprotective agents for LHON. Oral nicotinamide provides a robust neuroprotective effect against mitochondrial and metabolic dysfunction in other retinal injuries. We explored the potential for nicotinamide to protect mitochondria in LHON by modelling the disease in mice through intravitreal injection of the Complex I inhibitor rotenone. Using MitoV mice expressing a mitochondrial-tagged YFP in retinal ganglion cells we assessed mitochondrial morphology through super-resolution imaging and digital reconstruction. Rotenone induced Complex I inhibition resulted in retinal ganglion cell wide mitochondrial loss and fragmentation. This was prevented by oral nicotinamide treatment. Mitochondrial ultrastructure was quantified by transition electron microscopy, demonstrating a loss of cristae density following rotenone injection, which was also prevented by nicotinamide treatment. These results demonstrate that nicotinamide protects mitochondria during Complex I dysfunction. Nicotinamide has the potential to be a useful treatment strategy for LHON to limit retinal ganglion cell degeneration.
Long-term corrosion of copper alloys in the soil: new aspects of corrosion morphology in archaeological vessels from south-western Iran
Authors: Oudbashi Omid , Naseri Reza , Asadi Hasanvand Parnia
Published: March 2024

A group of copper-based objects excavated at Deh Dumen cemetery, in south-western Iran, was  studied and analysed to examine the long-term corrosion morphology and mechanism in the soil burial environment. For this purpose, twenty-two samples from twenty-one copper-based vessels were studied and analysed using X-ray diffraction, scanning electron microscopy—energy dispersive X-ray spectroscopy, micro-Raman spectroscopy and metallography techniques. The results of the analyses showed that the majority of vessels are made of tin bronze, along with two arsenical copper samples. The extent of corrosion observed ranges from very thin corrosion crusts to thick crusts and entirely corroded structures. These three identified corrosion morphologies display a multi-layered corrosion stratigraphy as well as the preserved limit of the original surface. The corrosion crusts include internal tin-rich and external copper-rich layers, and the main corrosion mechanism for the formation of multi-layered corrosion crusts is decuprification or selective dissolution of copper during the long-term burial time in a moderately Cl-contaminated soil. The three identified corrosion morphologies are similar to the previously published morphologies, but some clear deviations are apparent and are discussed here.
An engagement-aware predictive model to evaluate problem-solving performance from the study of adult skills' (PIAAC 2012) process data
Authors: Shin Jinnie , Wang Bowen , Pinto Junior Wallace N. , Gierl Mark J.
Published: March 2024

The benefits of incorporating process information in a large-scale assessment with the complex micro-level evidence from the examinees (i.e., process log data) are well documented in the research across large-scale assessments and learning analytics. This study introduces a deep-learning-based approach to predictive modeling of the examinee’s performance in sequential, interactive problem-solving tasks from a large-scale assessment of adults' educational competencies. The current methods disambiguate problem-solving behaviors using network analysis to inform the examinee's performance in a series of problem-solving tasks. The unique contribution of this framework lies in the introduction of an “effort-aware” system. The system considers the information regarding the examinee’s task-engagement level to accurately predict their task performance. The study demonstrates the potential to introduce a high-performing deep learning model to learning analytics and examinee performance modeling in a large-scale problem-solving task environment collected from the OECD Programme for the International Assessment of Adult Competencies (PIAAC 2012) test in multiple countries, including the United States, South Korea, and the United Kingdom. Our findings indicated a close relationship between the examinee's engagement level and their problem-solving skills as well as the importance of modeling them together to have a better measure of students’ problem-solving performance.
Development of personalized non-invasive ventilation masks for critically ill children: a bench study
Authors: Pigmans Rosemijne R. W. P. , Klein-Blommert Rozalinde , van Gestel Monica C. , Markhorst Dick G. , Hammond Peter , Boomsma Pim , Daams Tim , de Jong Julia M. A. , Heeman Paul M. , van Woensel Job B. M. , Dijkman Coen D. , Bem Reinout A.
Published: March 2024

Background

Obtaining a properly fitting non-invasive ventilation (NIV) mask to treat acute respiratory failure is a major challenge, especially in young children and patients with craniofacial abnormalities. Personalization of NIV masks holds promise to improve pediatric NIV efficiency. As current customization methods are relatively time consuming, this study aimed to test the air leak and surface pressure performance of personalized oronasal face masks using 3D printed soft materials. Personalized masks of three different biocompatible materials (silicone and photopolymer resin) were developed and tested on three head models of young children with abnormal facial features during preclinical bench simulation of pediatric NIV. Air leak percentages and facial surface pressures were measured and compared for each mask.

Results

Personalized NIV masks could be successfully produced in under 12 h in a semi-automated 3D production process. During NIV simulation, overall air leak performance and applied surface pressures were acceptable, with leak percentages under 30% and average surface pressure values mostly remaining under normal capillary pressure. There was a small advantage of the masks produced with soft photopolymer resin material.

Conclusion

This first, proof-of-concept bench study simulating NIV in children with abnormal facial features, showed that it is possible to obtain biocompatible, personalized oronasal masks with acceptable air leak and facial surface pressure performance using a relatively short, and semi-automated production process. Further research into the clinical value and possibilities for application of personalized NIV masks in critically ill children is needed.
Niche differentiation and biogeography of Bathyarchaeia in paddy soil ecosystems: a case study in eastern China
Authors: Yi Xingyun , Brandt Kristian Koefoed , Xue Shudan , Peng Jingjing , Wang Yifei , Li Meng , Deng Ye , Duan Guilan
Published: March 2024

Bathyarchaeia (formerly Bathyarchaeota) is a group of highly abundant archaeal communities that play important roles in global biogeochemical cycling. Bathyarchaeia is predominantly found in sediments and hot springs. However, their presence in arable soils is relatively limited. In this study, we aimed to investigate the spatial distributions and diversity of Bathyarchaeia in paddy soils across eastern China, which is a major rice production region. The relative abundance of Bathyarchaeia among total archaea ranged from 3 to 68% in paddy soils, and Bathy-6 was the dominant subgroup among the Bathyarchaeia (70–80% of all sequences). Bathyarchaeia showed higher migration ability and wider niche width based on the neutral and null model simulations. Bathy-6 was primarily assembled by deterministic processes. Soil pH and C/N ratio were identified as key factors influencing the Bathyarchaeia composition, whereas C/N ratio and mean annual temperature influenced the relative abundance of Bathyarchaeia. Network analysis showed that specific Bathyarchaeia taxa occupied keystone positions in the archaeal community and co-occurred with some methanogenic archaea, including Methanosarcina and Methanobacteria, and ammonia-oxidizing archaea belonging to Nitrososphaeria. This study provides important insights into the biogeography and niche differentiation of Bathyarchaeia particularly in paddy soil ecosystems.
Adherence to the nordic diet is associated with anxiety, stress, and depression in recovered COVID-19 patients, a case-control study
Authors: Araste Asie , Moghadam Mohammad Reza Shadmand Foumani , Mohammadhasani Kimia , Fard Mohammad Vahedi , Khorasanchi Zahra , Latifi MohammadReza , Hasanzadeh Elahe , Talkhi Nasrin , Sharifan Payam , Asadiyan-Sohan Parisa , Bidokhti Marjan Khayati , Ghassemi Arezoo , Darban Reza Assaran , Ferns Gordon , Ghayour-Mobarhan Majid
Published: March 2024

Background

Follow-up of COVID-19 recovered patients to discover important adverse effects on other organs is required. The psychological health of COVID-19 patients may be affected after recovery.

Aim

We aimed to evaluate the association between adherence to the Nordic diet (ND) and psychological symptoms caused by COVID-19 after recovery.

Method

Dietary data on 246 qualified adults (123 cases and 123 controls). The dietary intake in this case-control study was calculated by a reliable and valid food frequency questionnaire (FFQ). Depression Anxiety Stress Scale (DASS), Pittsburgh Sleep Quality Index (PSQI), Insomnia Severity Index (ISI), and Short-Form Health Survey (SF-36) were used to analyze participant’s anxiety, stress, depression, sleep quality, insomnia, and quality of life of participants.

Results

There was a significant inverse relationship between total anxiety, stress, and depression scores and the intake of whole grains (P < 0.05). Furthermore, there was a significant inverse association between depression and fruit intake (P < 0.05). A significant negative correlation was found between insomnia and sleep quality and the intake of root vegetables (P < 0.05). In the multinomial-regression model, a significant association between the Nordic diet and anxiety, stress, and depression was found only in the case group (OR = 0.719, 95% CI 0.563–0.918, p-value = 0.008; OR = 0.755, 95% CI 0.609–0.934, P-value = 0.010, and, OR = 0.759, 95% CI 0.602–0.956, P-value = 0.019 respectively).

Conclusion

Adherence to the Nordic diet might reduce anxiety, stress, and depression in recovered COVID-19 patients.
Pattern and determinants of tail-risk transmission between cryptocurrency markets: new evidence from recent crisis episodes
Authors: Maghyereh Aktham , Ziadat Salem Adel
Published: March 2024

The main objective of this study is to investigate tail risk connectedness among six major cryptocurrency markets and determine the extent to which investor sentiment, economic conditions, and economic uncertainty can predict tail risk interconnectedness. Combining the Conditional Autoregressive Value-at-Risk (CAViaR) model with the time-varying parameter vector autoregressive (TVP-VAR) approach shows that the transmission of tail risks among cryptocurrencies changes dynamically over time. During crises and significant events, transmission bursts and tail risks change. Based on both in- and out-of-sample forecasts, we find that the information contained in investor sentiment, economic conditions, and uncertainty includes significant predictive content about the tail risk connectedness of cryptocurrencies.
Does the issuance of green bonds nudge environmental responsibility engagements? Evidence from the Chinese green bond market
Authors: Liu Ying , Huang Hongyun , Mbanyele William , Wang Fengrong , Liu Huiling
Published: March 2024

Policymakers and managers have increasingly adopted green bonds as a direct financing tool to address environmental degradation and climate change in emerging economies; however, the increasing green washing sentiments in the green bond market raise questions on whether green bonds can nudge polluting businesses to achieve green transformation. Therefore, this study joins the controversial debate by investigating the impact of green bond issuance on corporate environmental responsibilities and the potential impact mechanisms and economic consequences. Using the data of Chinese listed enterprises from 2011 to 2020 and the staggered issuance of green bonds as plausibly exogenous shocks, we determine that the enterprises in the experimental group that issued green bonds increased their environmental performance compared to their counterparts. Furthermore, this positive link is maintains after a series of robustness tests. Moreover, we identify that green bond issuance plausibly enhances environmental responsibility engagements through two governance channels, namely, internal management and external supervision. This beneficial effect appears more pronounced for subsamples of firms in low-polluting industries, without environmental subsidies and with higher managerial abilities. Furthermore, economic consequences indicate that the issuance of green bonds primarily motivates speculative shareholder benefits, as evidenced by short-term increases in stock yields but with limited impact on the short-run financial performance. Overall, these findings offer new evidence supporting that green financing tools could play a helpful hand toward environmental sustainability.
Anti-immigrant backlash: the Democratic Dilemma for immigration policy
Authors: Kapelner Zsolt
Published: March 2024

Anti-immigrant backlash has emerged in recent years as a considerable threat to democracy. In many countries illiberal and anti-democratic political forces driven in part by anti-immigrant sentiment have damaged or threaten to damage the proper functioning of democratic institutions. Should policymakers try to avert this threat by implementing more restrictive immigration policy? If they do so, they may expose immigrants to unjust exclusion. If they do not, they may risk democratic dysfunction, even democratic failure. I will call this the Democratic Dilemma for immigration policy. In this paper I argue that this is a hard ethical dilemma that does not lend itself to a straightforward resolution. I propose an analytic and evaluative framework for assessing possible policy responses to the Dilemma to aid policymakers’ as well as the public’s ethical judgement.
The role of ambulatory blood pressure monitoring in enhancing medication adherence among patients with newly diagnosed hypertension: an analysis of the National Health Insurance cohort database
Authors: Kim Hack-Lyoung , Park So-Jeong , Bae Yoon-Jong , Ihm Sang Hyum , Shin Jinho , Kim Kwang-Il
Published: March 2024

Background

Improving adherence to antihypertensive medication (AHM) is a key challenge in hypertension management. This study aimed to assess the impact of ambulatory blood pressure monitoring (ABPM) on AHM adherence.

Methods

We utilized the Korean National Health Insurance Service database. Among patients newly diagnosed with hypertension who started AHM between July 2010 and December 2013, we compared clinical characteristics and adherence between 28,116 patients who underwent ABPM prior to starting AHM and 118,594 patients who did not undergo ABPM. Good adherence was defined as a proportion of days covered (PDC) of 0.8 or higher.

Results

The total study population was 146,710, with a mean age of 50.5 ± 6.4 years; 44.3% were female. Co-morbidities were noted in 4.2%. About a third of patients (33.1%) showed good adherence. The ABPM group had a notably higher PDC (total PDC: 0.64 ± 0.35 vs. 0.45 ± 0.39; P < 0.001), irrespective of the number of medications, dosing frequency, or prescription duration. After adjusting for significant clinical variables, ABPM was still closely linked with good adherence (odds ratio, 2.35; 95% confidence interval, 2.28–2.41; P < 0.001).

Conclusions

In newly diagnosed hypertension, undergoing ABPM prior to AHM prescription appears to enhance adherence to AHM. The exact mechanisms driving this association warrant further exploration.

Graphical Abstract
Uveal melanoma in the Iranian population: two decades of patient management in a tertiary eye center
Authors: Riazi-Esfahani Hamid , Amini Abdulrahim , Masoomian Babak , Yaseri Mehdi , Sabour Siamak , Rashidinia Ali , Arjmand Mojtaba , Rafizadeh Seyed Mohsen , Johari Mohammadkarim , Khalili Pour Elias , Ghassemi Fariba
Published: March 2024

Background

To assess the characteristics and outcomes of uveal melanoma management at a tertiary center in the Middle East.

Methods

A study on 164 patients with uveal melanoma was conducted by reviewing the available medical records, ultrasound, and pathology report results. Age at diagnosis, tumor location and size, treatment mode, visual outcome, metastasis, mortality, and survival were studied.

Results

The mean age of patients was 52.0 ± 15.0 years, and 52.5% were male. Choroidal melanoma was the most common uveal melanoma, followed by the ciliary body and iris melanoma. The mean thickness of tumors was 8.29 ± 3.29. The majority of patients (n = 111, 67.9%) were managed by brachytherapy with ruthenium-106 plaques. Enucleation was performed primarily in 46 (28%) patients and secondarily in nine (5.5%) patients. The sexual disparity was detected as the proximity of uveal melanoma to the fovea in males. For a 61-month mean follow-up period, mortality occurred in eight of our cases, six of which were due to metastasis. The most common site for distance metastasis was the liver (5/6), followed by the lung (1/6). The five-year and eight-year overall survival (OS) rate was 0.947%± 0.019. The 5-year survival rate reached zero in metastatic patients. OS was not statistically different depending on the age, tumor diameters, the primary treatment received, or the histopathologic findings (p > 0.50 for all).

Conclusion

In this study, individuals diagnosed with UM exhibited an OS rate of around 94% at the five-year mark, which remained consistent up to eight years. Notably, the presence of distance metastasis emerged as the sole statistically significant factor influencing overall survival.
Photovoltaic systems: a review with analysis of the energy transition in Brazilian culture, 2018–2023
Authors: David Thamyres Machado , de Souza Teófilo Miguel , Rizol Paloma Maria Silva Rocha
Published: March 2024

Countries all over the world have been seeking ways and methods so that their electrical matrices can stand out using clean and renewable energy sources. In this context, this article presents a review with analysis of sector legislation on photovoltaic solar energy in Brazil. This study was grounded in four steps: (i) sample definition; (ii) theoretical basis; (iii) network analysis; and (iv) content analysis in two stages of research. Initially, a systematic literature review was carried out in order to map all the major and most cited works. The second stage consisted in reading and performing a critical analysis of government documents and reports from the energy sector in Brazil using a few bibliometric resources for such a purpose. Its results reveal that photovoltaic solar energy in Brazil has grown and expanded to different applications, since floating solar plants and subscription to solar energy are becoming increasingly attractive. Furthermore, a possible replacement of photovoltaic solar generation for thermoelectric plants has been investigated once there are a few positive aspects yet to be found thereof. As samples of the results obtained, we have that the replacement of works would allow the photovoltaic solar energy source to increase by 1% in the electrical matrix and would stop emitting 10,738,478 tons into the atmosphere, there would be a progressive decrease in the use of tariff flags (which affect directly to the final consumer) and a reduction in operating costs would also be achieved.
Impact of numerous larval diets on the biology of pink bollworm Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) under laboratory conditions
Authors: Akhtar Shamim , Arif Muhammad Jalal , Gogi Muhammad Dildar , Haq Imran-ul
Published: March 2024

Background

Pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) has become a potential pest of cotton by causing substantial yield losses around the world including Pakistan. Keeping in view the facts like limited research investigations, unavailability, and high cost of artificial diet’s constituents and their premixes, the present research investigations on the dietary aspect of P. gossypiella were conducted. The larvae of P. gossypiella were reared on different diets that were prepared using indigenous elements. The standard/laboratory diet comprised of wheat germ meal 34.5 g, casein 30.0 g, agar–agar 20.0 g, sucrose 10.0 g, brewer’s yeast 5.0 g, α-cellulose 1.0 g, potassium-sorbate1.5 g, niplagin 0.5 g, decavitamin 0.01 g, choline-chloride 0.06 g, maize-oil 3.30 g, honey 2.0 g, and water 730.0 mL. Alternatives to cotton bolls and wheat germ meal were okra seed sprouts, okra fruit, cottonseed meal, and okra seed meals, which were included in the study to introduce an efficient and economic mass-rearing system.

Results

The larval development completed in 19.68 d ± 0.05 d with a weight of 20.18 mg ± 0.20 mg at the fourth instar fed on the cottonseed meal-based diet instead of wheat germ meal based diet. On the same diet, 84.00% ± 4.00%, 17.24 mg ± 0.03 mg, and 7.76 d ± 0.06 d were recorded as pupae formation, pupal weight, and pupal duration, respectively. Adult emergence, 76.00% ± 1.00% was recorded from pupae collected from larvae raised on cottonseed meal-based diet. These male and female moths lived for 40.25 d ± 0.10 d, and 44.34 d ± 0.11 d, respectively. Females deposited 21.28 ± 0.04 eggs per day with the viability of 65.78% ± 0.14%. The larval mortality at the fourth instar was 37.20% ± 1.36% and malformed pupation of 12.00% ± 1.41% was recorded. Replacement of wheat germ meal with that of local meals (cottonseed and okra seed) in the standard laboratory diet has saved 463.80 to 467.10 PKR with 1.62 to 1.63 cost economic returns, respectively.

Conclusion

This research is of novel nature as it provides a concise and workable system for the economic and successful rearing of P. gossypiella under laboratory conditions.
Practical solutions in fully homomorphic encryption: a survey analyzing existing acceleration methods
Authors: Gong Yanwei , Chang Xiaolin , Mišić Jelena , Mišić Vojislav B. , Wang Jianhua , Zhu Haoran
Published: March 2024

Fully homomorphic encryption (FHE) has experienced significant development and continuous breakthroughs in theory, enabling its widespread application in various fields, like outsourcing computation and secure multi-party computing, in order to preserve privacy. Nonetheless, the application of FHE is constrained by its substantial computing overhead and storage cost. Researchers have proposed practical acceleration solutions to address these issues. This paper aims to provide a comprehensive survey for systematically comparing and analyzing the strengths and weaknesses of FHE acceleration schemes, which is currently lacking in the literature. The relevant researches conducted between 2019 and 2022 are investigated. We first provide a comprehensive summary of the latest research findings on accelerating FHE, aiming to offer valuable insights for researchers interested in FHE acceleration. Secondly, we classify existing acceleration schemes from algorithmic and hardware perspectives. We also propose evaluation metrics and conduct a detailed comparison of various methods. Finally, our study presents the future research directions of FHE acceleration, and also offers both guidance and support for practical application and theoretical research in this field.
Targeted screening of atrial fibrillation using automated blood pressure measurement device with atrial fibrillation detection function, in patients with type 2 diabetes mellitus in primary care setting
Authors: Wong Yu Man , Chan Pang Fai , Lai Kit Ping Loretta , Luk Man Hei Matthew , Fung Hoi Tik , Yeung Sze Wai
Published: March 2024

Objective

The prevalence of atrial fibrillation (AF) in type 2 diabetes mellitus (DM) patients under primary care in Hong Kong was yet to be explored. We aimed to evaluate the prevalence of AF in patients with DM so as to provide evidence-based recommendations to incorporate AF screening as a component in regular diabetic risk and complication assessment. The performance of automated BP machine Microlife WatchBP Office AFIB as a screening tool for the detection of AF was also evaluated.

Method

This was a cross-sectional study. Patients with type 2 DM who attended the regular diabetic risk and complication assessment in the participating clinics from 24 August 2021 to 27 January 2022 were recruited. Blood pressure measurement by Microlife WatchBP Office AFIB and 12-lead ECGs were performed for AF screening.

Results

Among 2015 DM patients in primary care, the prevalence of AF was found to be 1.9% (95% confidence interval [CI] 1.3–2.6). The prevalence of AF increased with age, from 0.5% in patients aged < 65 years, to 2.2% in patients aged 65–74 years and 4.3% in patients aged ≥ 75 years. The sensitivity and specificity of Microlife WatchBP Office AFIB to detect AF were 80% (95% Cl 61.8–92.3) and 97.9%. (95% CI 97.3–98.5), respectively. The positive and negative predictive values were 32.8% (95% CI 21.9–45.1) and 99.7% (95% CI 99.5–99.9) respectively.

Conclusions

AF screening with the use of Microlife WatchBP Office AFIB is a simple procedure and can be considered as a standard assessment in the regular comprehensive diabetic risk and complication assessment in primary care setting.