bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of microRNAs in Obesity-Related Kidney Disease
Authors: Caus Maite , Eritja Àuria , Bozic Milica
Published: October 2021

Obesity is a major global health problem and is associated with a significant risk of renal function decline. Obesity-related nephropathy, as one of the complications of obesity, is characterized by a structural and functional damage of the kidney and represents one of the important contributors to the morbidity and mortality worldwide. Despite increasing data linking hyperlipidemia and lipotoxicity to kidney injury, the apprehension of molecular mechanisms leading to a development of kidney damage is scarce. MicroRNAs (miRNAs) are endogenously produced small noncoding RNA molecules with an important function in post-transcriptional regulation of gene expression. miRNAs have been demonstrated to be important regulators of a vast array of physiological and pathological processes in many organs, kidney being one of them. In this review, we present an overview of miRNAs, focusing on their functional role in the pathogenesis of obesity-associated renal pathologies. We explain novel findings regarding miRNA-mediated signaling in obesity-related nephropathies and highlight advantages and future perspectives of the therapeutic application of miRNAs in renal diseases.
The Effect of Sera from Children with Obstructive Sleep Apnea Syndrome (OSAS) on Human Cardiomyocytes Differentiated from Human Embryonic Stem Cells
Authors: Haddad Hen , Etzion Sharon , Rabinski Tatiana , Ofir Rivka , Regev Danielle , Etzion Yoram , Gopas Jacob , Goldbart Aviv
Published: October 2021

Obstructive sleep apnea syndrome (OSAS) patients suffer from cardiovascular morbidity, which is the leading cause of death in this disease. Based on our previous work with transformed cell lines and primary rat cardiomyocytes, we determined that upon incubation with sera from pediatric OSAS patients, the cell’s morphology changes, NF-κB pathway is activated, and their beating rate and viability decreases. These results suggest an important link between OSAS, systemic inflammatory signals and end-organ cardiovascular diseases. In this work, we confirmed and expanded these observations on a new in vitro system of beating human cardiomyocytes (CM) differentiated from human embryonic stem cells (hES). Our results show that incubation with pediatric OSAS sera, in contrast to sera from healthy children, induces over-expression of NF-κB p50 and p65 subunits, marked reduction in CMs beating rate, contraction amplitude and a strong reduction in intracellular calcium signal. The use of human CM cells derived from embryonic stem cells has not been previously reported in OSAS research. The results further support the hypothesis that NF-κB dependent inflammatory pathways play an important role in the evolution of cardiovascular morbidity in OSAS. This study uncovers a new model to investigate molecular and functional aspects of cardiovascular pathology in OSAS.
Comparative Antiseizure Analysis of Diverse Natural Coumarin Derivatives in Zebrafish
Authors: Kozioł Ewelina , Jóźwiak Krzysztof , Budzyńska Barbara , de Witte Peter A. M. , Copmans Daniëlle , Skalicka-Woźniak Krystyna
Published: October 2021

Coumarins are a well-known group of plant secondary metabolites with various pharmacological activities, including antiseizure activity. In the search for new antiseizure drugs (ASDs) to treat epilepsy, it is yet unclear which types of coumarins are particularly interesting as a systematic analysis has not been reported. The current study performed behavioral antiseizure activity screening of 18 different coumarin derivatives in the larval zebrafish pentylenetetrazole (PTZ) model using locomotor measurements. Activity was confirmed for seven compounds, which lowered seizure-like behavior as follows: oxypeucedanin 38%, oxypeucedanin hydrate 74%, notopterol 54%, nodakenetin 29%, hyuganin C 35%, daphnoretin 65%, and pimpinellin 60%. These coumarins, together with nodakenin, underwent further antiepileptiform analysis by local field potential recordings from the zebrafish opticum tectum (midbrain). All of them, except for nodakenetin, showed pronounced antiepileptiform activity, decreasing PTZ-induced elevation in power spectral density (PSD) by 83–89% for oxypeucedanin, oxypeucedanin hydrate, and notopterol, 77% for nodakenin, 26% for nodakenetin, 65% for hyuganin C, 88% for daphnoretin, and 81% for pimpinellin. These data demonstrate the potential of diverse coumarin scaffolds for ASD discovery. Finally, the structural differences between active and inactive coumarins were investigated in silico for oxypeucedanin hydrate and byacangelicin for their interaction with GABA-transaminase, a hypothetical target.
The Role of Long Non-Coding RNAs in Endometriosis
Authors: Hudson Quanah J. , Proestling Katharina , Perricos Alexandra , Kuessel Lorenz , Husslein Heinrich , Wenzl René , Yotova Iveta
Published: October 2021

Endometriosis is a chronic gynecological disorder affecting the quality of life and fertility of many women around the world. Heterogeneous and non-specific symptoms may lead to a delay in diagnosis, with treatment options limited to surgery and hormonal therapy. Hence, there is a need to better understand the pathogenesis of the disease to improve diagnosis and treatment. Long non-coding RNAs (lncRNAs) have been increasingly shown to be involved in gene regulation but remain relatively under investigated in endometriosis. Mutational and transcriptomic studies have implicated lncRNAs in the pathogenesis of endometriosis. Single-nucleotide polymorphisms (SNPs) in lncRNAs or their regulatory regions have been associated with endometriosis. Genome-wide transcriptomic studies have identified lncRNAs that show deregulated expression in endometriosis, some of which have been subjected to further experiments, which support a role in endometriosis. Mechanistic studies indicate that lncRNAs may regulate genes involved in endometriosis by acting as a molecular sponge for miRNAs, by directly targeting regulatory elements via interactions with chromatin or transcription factors or by affecting signaling pathways. Future studies should concentrate on determining the role of uncharacterized lncRNAs revealed by endometriosis transcriptome studies and the relevance of lncRNAs implicated in the disease by in vitro and animal model studies.
Evaluating a Taxonomy of Textual Uncertainty for Collaborative Visualisation in the Digital Humanities
Authors: Benito-Santos Alejandro , Doran Michelle , Rocha Aleyda , Wandl-Vogt Eveline , Edmond Jennifer , Therón Roberto
Published: October 2021

The capture, modelling and visualisation of uncertainty has become a hot topic in many areas of science, such as the digital humanities (DH). Fuelled by critical voices among the DH community, DH scholars are becoming more aware of the intrinsic advantages that incorporating the notion of uncertainty into their workflows may bring. Additionally, the increasing availability of ubiquitous, web-based technologies has given rise to many collaborative tools that aim to support DH scholars in performing remote work alongside distant peers from other parts of the world. In this context, this paper describes two user studies seeking to evaluate a taxonomy of textual uncertainty aimed at enabling remote collaborations on digital humanities (DH) research objects in a digital medium. Our study focuses on the task of free annotation of uncertainty in texts in two different scenarios, seeking to establish the requirements of the underlying data and uncertainty models that would be needed to implement a hypothetical collaborative annotation system (CAS) that uses information visualisation and visual analytics techniques to leverage the cognitive effort implied by these tasks. To identify user needs and other requirements, we held two user-driven design experiences with DH experts and lay users, focusing on the annotation of uncertainty in historical recipes and literary texts. The lessons learned from these experiments are gathered in a series of insights and observations on how these different user groups collaborated to adapt an uncertainty taxonomy to solve the proposed exercises. Furthermore, we extract a series of recommendations and future lines of work that we share with the community in an attempt to establish a common agenda of DH research that focuses on collaboration around the idea of uncertainty.
Epigenetic State Changes Underlie Metabolic Switch in Mouse Post-Infarction Border Zone Cardiomyocytes
Authors: Günthel Marie , van Duijvenboden Karel , de Bakker Dennis E. M. , Hooijkaas Ingeborg B. , Bakkers Jeroen , Barnett Phil , Christoffels Vincent M.
Published: October 2021

Myocardial infarction causes ventricular muscle loss and formation of scar tissue. The surviving myocardium in the border zone, located adjacent to the infarct, undergoes profound changes in function, structure and composition. How and to what extent these changes of border zone cardiomyocytes are regulated epigenetically is not fully understood. Here, we obtained transcriptomes of PCM-1-sorted mouse cardiomyocyte nuclei of healthy left ventricle and 7 days post myocardial infarction border zone tissue. We validated previously observed downregulation of genes involved in fatty acid metabolism, oxidative phosphorylation and mitochondrial function in border zone-derived cardiomyocytes, and observed a modest induction of genes involved in glycolysis, including Slc2a1 (Glut1) and Pfkp. To gain insight into the underlying epigenetic regulatory mechanisms, we performed H3K27ac profiling of healthy and border zone cardiomyocyte nuclei. We confirmed the switch from Mef2- to AP-1 chromatin association in border zone cardiomyocytes, and observed, in addition, an enrichment of PPAR/RXR binding motifs in the sites with reduced H3K27ac signal. We detected downregulation and accompanying epigenetic state changes at several key PPAR target genes including Ppargc1a (PGC-1α), Cpt2, Ech1, Fabpc3 and Vldrl in border zone cardiomyocytes. These data indicate that changes in epigenetic state and gene regulation underlie the maintained metabolic switch in border zone cardiomyocytes.
Surgical Strategy for the Management of Cervical Deformity Is Based on Type of Cervical Deformity
Authors: Kim Han Jo , Virk Sohrab , Elysee Jonathan , Ames Christopher , Passias Peter , Shaffrey Christopher , Mundis Gregory , Protopsaltis Themistocles , Gupta Munish , Klineberg Eric , Hart Robert , Smith Justin S. , Bess Shay , Schwab Frank , Lafage Renaud , Lafage Virginie ,   on behalf of the International Spine Study Group
Published: October 2021

Objectives: Cervical deformity morphotypes based on type and location of deformity have previously been described. This study aimed to examine the surgical strategies implemented to treat these deformity types and identify if differences in treatment strategies impact surgical outcomes. Our hypothesis was that surgical strategies will differ based on different morphologies of cervical deformity. Methods: Adult patients enrolled in a prospective cervical deformity database were classified into four deformity types (Flatneck (FN), Focal kyphosis (FK), Cervicothoracic kyphosis (CTK) and Coronal (C)), as previously described. We analyzed group differences in demographics, preoperative symptoms, health-related quality of life scores (HRQOLs), and surgical strategies were evaluated, and postop radiographic and HROQLs at 1+ year follow up were compared. Results: 90/109 eligible patients (mean age 63.3 ± 9.2, 64% female, CCI 1.01 ± 1.36) were evaluated. Group distributions included FN = 33%, FK = 29%, CTK = 29%, and C = 9%. Significant differences were noted in the surgical approaches for the four types of deformities, with FN and FK having a high number of anterior/posterior (APSF) approaches, while CTK and C had more posterior only (PSF) approaches. For FN and FK, PSF was utilized more in cases with prior anterior surgery (70% vs. 25%). For FN group, PSF resulted in inferior neck disability index compared to those receiving APSF suggesting APSF is superior for FN types. CTK types had more three-column osteotomies (3CO) (p < 0.01) and longer fusions with the LIV below T7 (p < 0.01). There were no differences in the UIV between all deformity types (p = 0.19). All four types of deformities had significant improvement in NRS neck pain post-op (p < 0.05) with their respective surgical strategies. Conclusions: The four types of cervical deformities had different surgical strategies to achieve improvements in HRQOLs. FN and FK types were more often treated with APSF surgery, while types CTK and C were more likely to undergo PSF. CTK deformities had the highest number of 3COs. This information may provide guidelines for the successful management of cervical deformities.
Prevalence of Neural Autoantibodies in Paired Serum and Cerebrospinal Fluid in Adult Patients with Drug-Resistant Temporal Lobe Epilepsy of Unknown Etiology
Authors: Cabezudo-García Pablo , Ciano-Petersen Nicolás L. , Mena-Vázquez Natalia , Ortega-Pinazo Jesús , Postigo-Pozo María J. , García-Martín Guillermina , Antolí-Martínez Helena , Sánchez-Sánchez Violeta , Quiroga-Subirana Pablo , Serrano-Castro Pedro J. , Estivill-Torrús Guillermo
Published: October 2021

In order to determine the prevalence of neural autoantibodies in adult patients with drug-resistant temporal lobe epilepsy (DRTLE) of unknown etiology, we compared the characteristics of patients with and without autoantibodies and applied antibody predictive scores to the patients. Patients aged ≥18 years with DRTLE of unknown etiology and ≥12 months of evolution were prospectively recruited. Neural autoantibodies in serum and CSF were systematically determined in all patients. We created the ARTE (antibody in drug-resistant temporal lobe epilepsy) score based on the variables associated with the presence of neural autoantibodies. Twenty-seven patients were included. The mean (SD) age in years at the index date was 52 (±14.2) and at epilepsy onset was 32 (±17.1). The mean epilepsy duration was 19 (±12.5) years. Neural autoantibodies were detected in 51.85% (14/27) of patients. The presence of bitemporal, independent, interictal epileptiform discharges (BIIED) had a higher frequency in patients with neural autoantibodies (57.1% vs. 15.4%; p = 0.025) as well as those patients with a previous history of status epilepticus (49.2% vs. 0.0%; p = 0.007). The ARTE score showed an area under the curve (AUC) of 0.854. Using a cut-off point of ≥1, the sensitivity was 100% and the specificity was 46.1%, whereas when using a cut-off point of ≥3, the results were 35.7% and 100%, respectively. We found a high prevalence of neural autoantibodies in patients with DRTLE of unknown etiology, indicating an autoimmune mechanism. The presence of BIIED and a history of SE in DRTLE of unknown etiology are possible markers for autoimmune-associated epilepsy. The proposed ARTE score requires future validation in larger independent cohorts.
Sentinel Lymph Node Staging with Indocyanine Green for Patients with Cervical Cancer: The Safety and Feasibility of Open Approach Using SPY-PHI Technique
Authors: Muallem Mustafa Zelal , Sayasneh Ahmad , Armbrust Robert , Sehouli Jalid , Miranda Andrea
Published: October 2021

(1) Background: Sentinel lymph node staging (SLN) with indocyanine green (ICG) in cervical cancer is the standard of care in most national and international guidelines. However, the vast majority of relevant studies about the safety and feasibility of this method are conducted on minimally invasive surgery; (2) Methods: This study is a retrospective analysis of a retrospective collected database of 76 consecutive patients with cervical cancers, who were operated laparoscopically (50%), or laparotomy (50%). Sentinel nodes were defined as the ICG-positive pelvic nodes in the first and second echelons. False negative cases were defined as positive non-sentinel lymph nodes despite successful sentinel mapping or failed mapping bilaterally by per-patient assessment or unilaterally by pelvic sidewall assessment; (3) Results: Regardless of the approach (open or laparoscopic), the SLN technique achieved a total sensitivity, specificity, and negative predictive value (NPV) of 94.7%, 98.6%, and 94.7%, respectively in the entire sample. The bilateral detection rate was as high as 93.4% with identical results in both approaches. The sensitivity and NPV for SNL in open surgery was found to be similar to minimal access surgery; (4) Conclusions: ICG and SPY-PHI technique is a reliable tool to detect sentinel lymph nodes in cervical cancer during laparotomy.
Effect of Postoperative Compression Therapy on the Success of Liposuction in Patients with Advanced Lower Limb Lymphedema
Authors: Yoshida Shuhei , Koshima Isao , Imai Hirofumi , Roh Solji , Mese Toshiro , Uchiki Toshio , Sasaki Ayano , Nagamatsu Shogo
Published: October 2021

Objective: There is limited information on postoperative care after liposuction for lymphedema limb. The aim of this retrospective study was to identify the threshold compression pressure and other factors that lead liposuction for lower limb lymphedema to success. Materials and Methods: Patients were divided according to whether they underwent compression therapy with both stockings and bandaging (SB group), stockings alone (S group), or bandaging alone (B group) for 6 months after liposuction. The postoperative compression pressure and rate of improvement were compared according to the postoperative compression method. We also investigated whether it was possible to decrease the compression pressure after 6 months. Liposuction was considered successful if improvement rate was >15. Results: Mean compression pressure was significantly lower in the S group than in the SB group or B group. The liposuction success rate was significantly higher in the SB group than in the B group or S group. There was not a significant difference between the values at 6 months after liposuction and at 6 months after a decrease in compression pressure in the successful group. Conclusion: Our results suggest that stable high-pressure postoperative compression therapy is key to the success of liposuction for lower limb lymphedema and is best achieved by using both stockings and bandages. The postoperative compression pressure required for liposuction to be successful was >40 mmHg on the lower leg and >20 mmHg on the thigh. These pressures could be decreased after 6 months.
Effect of Adding Midazolam to Dual Prophylaxis for Preventing Postoperative Nausea and Vomiting
Authors: Lee Jiyoung , Yi In Kyong , Han Jung Youn , Na Eun Duc , Park Chunghyun , Kim Jong Yeop
Published: October 2021

Multimodal prophylaxis for postoperative nausea and vomiting (PONV) has been recommended, even in low-risk patients. Midazolam is known to have antiemetic properties. We researched the effects of adding midazolam to the dual prophylaxis of ondansetron and dexamethasone on PONV after gynecologic laparoscopy. In this prospective, randomized, double-blinded trial, 144 patients undergoing gynecological laparoscopic surgery under sevoflurane anesthesia were randomized to receive either normal saline (control group, n = 72) or midazolam 0.05 mg/kg (midazolam group, n = 72) intravenously at pre-induction. All patients were administered dexamethasone 4 mg at induction and ondansetron 4 mg at the completion of the laparoscopy, intravenously. The primary outcome was the incidence of complete response, which implied the absence of PONV without rescue antiemetic requirement until 24 h post-surgery. The complete response during the 24 h following laparoscopy was similar between the two groups: 41 patients (59%) in the control group and 48 patients (72%) in the midazolam group (p = 0.11). The incidence of nausea, severe nausea, retching/vomiting, and administration of rescue antiemetic was comparable between the two groups. The addition of 0.05 mg/kg midazolam at pre-induction to the dual prophylaxis had no additive preventive effect on PONV after gynecologic laparoscopy.
Validating Consensus-Defined Severity Grading of Lymphatic Complications after Kidney Transplant
Authors: Sabagh Mohammadsadegh , Mohammadi Sara , Ramouz Ali , Khajeh Elias , Ghamarnejad Omid , Morath Christian , Mieth Markus , Kulu Yakup , Zeier Martin , Mehrabi Arianeb , Golriz Mohammad
Published: October 2021

Lymphatic complications after kidney transplantation (KTx) are associated with morbidities such as impaired wound healing, thrombosis, and organ failure. Recently, a consensus regarding the definition and severity grading of lymphoceles has been suggested. The aim of the present study was to validate this classification method. All adult patients who underwent KTx between December 2011 and September 2016 in our department were evaluated regarding lymphoceles that were diagnosed within 6 months after KTx based on the recent definition. Patients with lymphoceles were categorized according to the classification criteria, and clinical outcomes were compared between the groups. In our department, a total of 587 patients underwent KTx between 2011 and 2016. Lymphoceles were detected after KTx in 90 patients (15.3%). Among these patients, 24 (26.6%) had grade A lymphoceles, 14 (15.6%) had grade B, and 52 (57.8%) had grade C. The median duration times of intermediate care (IMC) and hospital stay were significantly higher among patients with grade C lymphoceles than they were among patients with grade A and B lymphoceles. Significantly more patients with grade C lymphoceles were readmitted to the hospital for treatment. The recently published definition and severity grading of lymphoceles after KTx is an easy-to-use and valid classification system, which may facilitate the comparison of results from different studies on lymphoceles after KTx.
Sonographic Evaluation of Gastric Residual Volume during Enteral Nutrition in Critically Ill Patients Using a Miniaturized Ultrasound Device
Authors: Jahreis Tizian , Kretschmann Jessica , Weidner Nick , Volk Thomas , Meiser Andreas , Groesdonk Heinrich Volker
Published: October 2021

Background: To assess the risk of aspiration, nutrient tolerance, and gastric emptying of patients in ICUs, gastric ultrasound can provide information about the gastric contents. Using established formulas, the gastric residual volume (GRV) can be calculated in a standardized way by measuring the gastric antrum. The purpose of this study was to determine the GRV in a cohort of enterally fed patients using a miniaturized ultrasound device to achieve knowledge about feasibility and the GRV over time during the ICU stay. The findings could contribute to the optimization of enteral nutrition (EN) therapy. Methods: A total of 217 ultrasound examinations with 3 measurements each (651 measurements in total) were performed twice daily (morning and evening) in a longitudinal observational study on 18 patients with EN in the interdisciplinary surgical ICU of Saarland University Medical Center. The measured values of the GRV were analyzed in relation to the clinical course, the nutrition, and other parameters. Results: Measurements could be performed without interrupting the flow of clinical care and without pausing EN. The GRV was significantly larger with sparsely auscultated bowel sounds than with normal and excited bowel sounds (p < 0.01). Furthermore, a significantly larger GRV was present when using a high-caloric/low-protein nutritional product compared to an isocaloric product (p = 0.02). The GRV at the morning and evening measurements showed no circadian rhythm. When comparing the first and last ultrasound examination of each patient, there was a tendency towards an increased GRV (p = 0.07). Conclusion: The GRV measured by miniaturized ultrasound devices can provide important information about ICU patients without restricting treatment procedures in the ICU. Measurements are possible while EN therapy is ongoing. Further studies are needed to establish gastric ultrasound as a management tool in nutrition therapy.
Right Ventricular Structure and Function in Young Adults Born Preterm at Very Low Birth Weight
Authors: Greer Charlotte , Harris Sarah L. , Troughton Richard , Adamson Philip D. , Horwood John , Frampton Chris , Darlow Brian A.
Published: October 2021

Being born preterm (PT, <37 weeks gestation) or at very low birth weight (VLBW, <1500 g) is associated with increased rates of cardiopulmonary disorders in childhood. As survivors age, late cardiac effects, including right ventricular (RV) remodelling and occult pulmonary hypertension are emerging. In this population-based study, we aimed to investigate right heart structure and function in young adults born PT at VLBW compared to normal-weight term-born controls. The New Zealand VLBW Study has followed all infants born in 1986 with birth weight <1500 g. All were born preterm from 24 to 37 weeks. A total of 229 (71% of survivors) had echocardiograms aged 26–30 years which were compared to age-matched, term-born, normal-weight controls (n = 100). Young adults born preterm at very low birth weight exhibited smaller RV dimensions compared to term-born peers. Standard echocardiographic measures of RV function did not differ, but mildly reduced function was detected by RV longitudinal strain. This difference was related to birth weight and gestational age but not lung function or left ventricular function. Echocardiographic strain imaging may be an important tool to detect differences in RV function preterm and VLBW.
Association of Metabolic Syndrome with Sensorineural Hearing Loss
Authors: Rim Hwa-Sung , Kim Myung-Gu , Park Dong-Choon , Kim Sung-Soo , Kang Dae-Woong , Kim Sang-Hoon , Yeo Seung-Geun
Published: October 2021

The prevalence of sensorineural hearing loss has increased along with increases in life expectancy and exposure to noisy environments. Metabolic syndrome (MetS) is a cluster of co-occurring conditions that increase the risk of heart disease, stroke and type 2 diabetes, along with other conditions that affect the blood vessels. Components of MetS include insulin resistance, body weight, lipid concentration, blood pressure, and blood glucose concentration, as well as other features of insulin resistance such as microalbuminuria. MetS has become a major public health problem affecting 20–30% of the global population. This study utilized health examination to investigate whether metabolic syndrome was related to hearing loss. Methods: A total of 94,223 people who underwent health check-ups, including hearing tests, from January 2010 to December 2020 were evaluated. Subjects were divided into two groups, with and without metabolic syndrome. In addition, Scopus, Embase, PubMed, and Cochrane libraries were systematically searched, using keywords such as “hearing loss” and “metabolic syndrome”, for studies that evaluated the relationship between the two. Results: Of the 94,223 subjects, 11,414 (12.1%) had metabolic syndrome and 82,809 did not. The mean ages of subjects in the two groups were 46.1 and 43.9 years, respectively. A comparison of hearing thresholds by age in subjects with and without metabolic syndrome showed that the average pure tone hearing thresholds were significantly higher in subjects with metabolic syndrome than in subjects without it in all age groups. (p < 0.001) Rates of hearing loss in subjects with 0, 1, 2, 3, 4, and 5 of the components of metabolic syndrome were 7.9%, 12.1%, 13.8%, 13.8%, 15.5% and 16.3%, respectively, indicating a significant association between the number of components of metabolic syndrome and the rate of hearing loss (p < 0.0001). The odds ratio of hearing loss was significantly higher in subjects with four components of metabolic syndrome: waist circumference, blood pressure, and triglyceride and fasting blood sugar concentrations (p < 0.0001). Conclusions: The number of components of the metabolic syndrome is positively correlated with the rate of sensorineural hearing loss.
Chart Classification Using Siamese CNN
Authors: Bajić Filip , Job Josip
Published: October 2021

In recovering information from the chart image, the first step should be chart type classification. Throughout history, many approaches have been used, and some of them achieve results better than others. The latest articles are using a Support Vector Machine (SVM) in combination with a Convolutional Neural Network (CNN), which achieve almost perfect results with the datasets of few thousand images per class. The datasets containing chart images are primarily synthetic and lack real-world examples. To overcome the problem of small datasets, to our knowledge, this is the first report of using Siamese CNN architecture for chart type classification. Multiple network architectures are tested, and the results of different dataset sizes are compared. The network verification is conducted using Few-shot learning (FSL). Many of described advantages of Siamese CNNs are shown in examples. In the end, we show that the Siamese CNN can work with one image per class, and a 100% average classification accuracy is achieved with 50 images per class, where the CNN achieves only average classification accuracy of 43% for the same dataset.
The Design Methodology of Fully Digital Pulse Width Modulation
Authors: Shahroury Fadi R.
Published: October 2021

This paper describes the design methodology and calibration technique for a low-power digital pulse width modulation demodulator to enhance its robustness against the process, voltage, and temperature variations in different process corners, in addition to intra-die variability, which makes it a very good choice for implantable monitoring sensors. Furthermore, the core of the proposed demodulator is fully digital. Thus, along with the proposed design methodology, the proposed demodulator can be simply redesigned in advanced subnanometer CMOS technologies without much difficulty as compared to analog demodulators. The proposed demodulator consists of an envelope detector, a digitizer, a ring oscillator, and a data detector with digital calibration. All the proposed circuits are designed and simulated in the standard 1P9M TSMC’s 40 nm CMOS technology. Simulation results have shown that the circuit is capable of demodulating and recovering data from an input signal with a carrier frequency of 13.56 MHz and a data rate of 143 kB/s with an average power consumption of 5.62 μW.
An Investigation of Adaptive Radius for the Covariance Localization in Ensemble Data Assimilation
Authors: Xing Xiang , Liu Bainian , Zhang Weimin , Wu Jianping , Cao Xiaoqun , Huang Qunbo
Published: October 2021

The covariance matrix estimated from the ensemble data assimilation always suffers from filter collapse because of the spurious correlations induced by the finite ensemble size. The localization technique is applied to ameliorate this issue, which has been suggested to be effective. In this paper, an adaptive scheme for Schur product covariance localization is proposed, which is easy and efficient to implement in the ensemble data assimilation frameworks. A Gaussian-shaped taper function is selected as the localization taper function for the Schur product in the adaptive localization scheme, and the localization radius is obtained adaptively through a certain criterion of correlations with the background ensembles. An idealized Lorenz96 model with an ensemble Kalman filter is firstly examined, showing that the adaptive localization scheme helps to significantly reduce the spurious correlations in the small ensemble with low computational cost and provides accurate covariances that are similar to those derived from a much larger ensemble. The investigations of adaptive localization radius reveal that the optimal radius is model-parameter-dependent, vertical-level-dependent and nearly flow-dependent with weather scenarios in a realistic model; for example, the radius of model parameter zonal wind is generally larger than that of temperature. The adaptivity of the localization scheme is also illustrated in the ensemble framework and shows that the adaptive scheme has a positive effect on the assimilated analysis as the well-tuned localization.
High Skin Sympathetic Nerve Activity in Patients with Recurrent Syncope
Authors: Huang Tien-Chi , Chi Nai-Yu , Lan Chih-Sung , Chen Chang-Jen , Jhuo Shih-Jie , Lin Tsung-Han , Liu Yi-Hsueh , Chou Li-Fang , Chang Chien-Wei , Liao Wei-Sheng , Kao Pei-Heng , Hsu Po-Chao , Lee Chee-Siong , Lin Yi-Hsiung , Lee Hsiang-Chun , Lu Ye-Hsu , Yen Hsueh-Wei , Lin Tsung-Hsien , Su Ho-Ming , Lai Wen-Ter , Tsai Wei-Chung , Lin Shien-Fong , Lee Chien-Hung
Published: October 2021

(1) Background: The autonomic imbalance plays a role in vasovagal syncope (VVS) diagnosed by head-up tilting test (HUT). neuECG is a new method of recording skin electrical signals to simultaneously analyze skin sympathetic nerve activity (SKNA) and electrocardiogram. We hypothesize that SKNA is higher in subjects with tilt-positive than tilt-negative and the SKNA surges before syncope. (2) Methods: We recorded neuECG in 41 subjects who received HUT (according to the “Italian protocol”), including rest, tilt-up, provocation and recovery phases. Data were analyzed to determine the average SKNA (aSKNA, μV) per digitized sample. Electrocardiogram was used to calculate standard deviation of normal-to-normal beat intervals (SDNN). The “SKNA-SDNN index” was calculated by rest aSKNA multiplied by the ratio of tilt-up to rest SDNN. (3) Results: 16 of 41 (39%) subjects developed syncope. The aSKNA at rest phase is significantly higher in the tilt-positive (1.21 ± 0.27 µV) than tilt-negative subjects (1.02 ± 0.29 µV) (p = 0.034). There are significant surges and withdraw of aSKNA 30 s before and after syncope (both p ≤ 0.006). SKNA-SDNN index is able to predict syncope (p < 0.001). (4) Conclusion: Higher SKNA at rest phase is associated with positive HUT. The SKNA-SDNN index is a novel marker to predict syncope during HUT.
Prefrontal Lobe and Posterior Cingulate Cortex Activations in Patients with Major Depressive Disorder by Using Standardized Weighted Low-Resolution Electromagnetic Tomography
Authors: Lin I-Mei , Yu Hong-En , Yeh Yi-Chun , Huang Mei-Feng , Wu Kuan-Ta , Ke Chiao-Li Khale , Lin Pei-Yun , Yen Cheng-Fang
Published: October 2021

Background: The differences in brain activity between patients with major depressive disorder (MDD) and healthy adults have been confirmed by functional magnetic resonance imaging (fMRI), positron emission tomography (PET), and electroencephalography (EEG). The prefrontal lobe and posterior cingulate cortex (PCC) are related to emotional regulation in patients with MDD. However, the high cost and poor time resolution of fMRI and PET limit their clinical application. Recently, researchers have used high time resolution of standardized weighted low-resolution electromagnetic tomography (swLORETA) to investigate deep brain activity. This study aimed to convert raw EEG signals into swLORETA images and explore deep brain activity in patients with MDD and healthy adults. Methods: BrainMaster EEG equipment with a 19-channel EEG cap was used to collect resting EEG data with eyes closed for 5 min. NeuroGuide software was used to remove the EEG artifacts, and the swLORETA software was used to analyze 12,700 voxels of current source density (CSD) for 139 patients with MDD and co-morbid anxiety symptoms (mean age = 43.08, SD = 13.76; 28.78% were male) and 134 healthy adults (mean age = 40.60, SD = 13.52; 34.33% were male). Deep brain activity in the frontal lobe and PCC at different frequency bands was analyzed, including delta (1–4 Hz), theta (5–7 Hz), alpha (8–11 Hz), beta (12–24 Hz), beta1 (12–14 Hz), beta2 (15–17 Hz), beta3 (18–24 Hz), and high beta (25–29 Hz). Results: There was lower delta and theta and higher beta, beta1, beta2, beta3, and high-beta activity at the prefrontal lobe (dorsal medial prefrontal cortex [dmPFC], ventral medial prefrontal cortex [vmPFC], and dorsal lateral prefrontal cortex [dlPFC], ventral lateral prefrontal cortex [vlPFC], orbital frontal cortex [OFC]) and PCC in MDD patients compared with healthy adults. There was no significant difference in alpha activity between the two groups. Conclusion: This study indicates brain hyperactivity in the right prefrontal lobe (dlPFC and vmPFC) and PCC in patients with MDD with co-morbid anxiety symptoms, and the dlPFC and PCC were also related to emotion regulation in MDD. Inhibiting high-beta activity or restoring delta and theta activity to the normal range in the right frontal lobe and PCC may be possible in z-score neurofeedback protocols for patients with MDD in future studies.
Multiphoton Microscopy for Identifying Collagen Signatures Associated with Biochemical Recurrence in Prostate Cancer Patients
Authors: Pavlova Ina P. , Nair Sujit S. , Lundon Dara , Sobotka Stanislaw , Roshandel Reza , Treacy Patrick-Julien , Ratnani Parita , Brody Rachel , Epstein Jonathan I. , Ayala Gustavo E. , Kyprianou Natasha , Tewari Ashutosh K.
Published: October 2021

Prostate cancer is a heterogeneous disease that remains dormant for long periods or acts aggressively with poor clinical outcomes. Identifying aggressive prostate tumor behavior using current glandular-focused histopathological criteria is challenging. Recent evidence has implicated the stroma in modulating prostate tumor behavior and in predicting post-surgical outcomes. However, the emergence of stromal signatures has been limited, due in part to the lack of adoption of imaging modalities for stromal-specific profiling. Herein, label-free multiphoton microscopy (MPM), with its ability to image tissue with stromal-specific contrast, is used to identify prostate stromal features associated with aggressive tumor behavior and clinical outcome. MPM was performed on unstained prostatectomy specimens from 59 patients and on biopsy specimens from 17 patients with known post-surgery recurrence status. MPM-identified collagen content, organization, and morphological tumor signatures were extracted for each patient and screened for association with recurrent disease. Compared to tumors from patients whose disease did not recur, tumors from patients with recurrent disease exhibited higher MPM-identified collagen amount and collagen fiber intensity signal and width. Our study shows an association between MPM-identified stromal collagen features of prostate tumors and post-surgical disease recurrence, suggesting their potential for prostate cancer risk assessment.
Does the Disclosure of an Audit Engagement Partner’s Name Improve the Audit Quality? A Difference-in-Difference Analysis
Authors: John Kose , Liu Min (Shirley)
Published: October 2021

On 15 December 2015, the Public Company Accounting Oversight Board (PCAOB) passed Rule 3211, requiring audit firms registered with PCAOB in the U.S. to disclose the audit engagement partner’s name in the Form AP, effective 31 January 2017. The regulation aims to improve the transparency and quality of audits, thereby increasing investors’ confidence in financial statements. Using the audit firms registered with the PCAOB and their clients as the treated sample, we employed a difference-in-difference analysis to investigate whether and the extent to which implementing Rule 3211 impacts audit quality and audit costs. We compared the audit quality (proxied by the abnormal discretionary accruals quality, the probability of restating the financial statements, and the ratio of the audit fees to the total fees) and audit costs (proxied by the total audit fees) from one year (up to three years) pre- to one year (up to three years) post-Rule 3211, to a control sample (comprised of U.K. audit firms, which were not subject to such regulation during the sample period). The empirical results generally indicate that there was an increase in the audit quality and in the audit costs from the pre- to the post-Rule 3211 period and also suggest that auditor independence increased in the post-regulation period compared to the pre-regulation period. Our empirical results are new and contribute to the research on the PCAOB and audits.
Coupling Coordination Analysis of Livelihood Efficiency and Land Use for Households in Poverty-Alleviated Mountainous Areas
Authors: Su Fang , Chang Jiangbo , Shang Haiyang
Published: October 2021

The interaction between livelihood means and land use pattern of households is the core of the interactive coupling of the human-land system. This study focuses on Qinba mountainous area in southern Shaanxi province, a typical poverty-alleviated mountainous area. With the help of the coupling coordination degree model, kernel density estimation, and trend surface analysis, this study constructs the coupling coordination degree of livelihood efficiency and land use for households, and analyzes the differences between households’ livelihood efficiency and land use level, as well as the coupling coordination relationship between households livelihood efficiency and land use in different types and regions. The research conclusions are as follows. (1) For households in the Qinba mountainous area, southern Shaanxi province, the livelihood efficiency is at a medium level of 0.681, the land use is at a low level of 0.127, while the coupling coordination degree 0.526 is at the primary coordination state. (2) With the increase of nonagricultural degree, the coupling coordination degree of households increases first, and then decreases. (3) The coupling coordination degree for households east-to-west is “sagging”, while south-to-north diagram is “hogging”. (4) The distribution of the coupling coordination degree for agriculture-dependent households east-to-west (the “sagging” diagram) is opposite to the other types of households. By analogy, the distribution of the coupling coordination degree for nonagriculture and agriculture-dependent households north-to-south (the “hogging” diagram) is opposite to the other types of households. The coupling coordination between the households’ livelihood efficiency and land use level is affected by the households’ regional development level, natural resources, geographic location, infrastructure availability and many other factors. Making appropriate livelihood development plans based on the types of households and regional space can both effectively improve the livelihood conditions, as well as offer guidance in promoting regional human-land activity coordination and ensuring sustainable development.
Fertile Ground: Implementing the 2030 Agenda in U.S. Cities
Authors: Stoker Robert P. , Rich Michael J.
Published: October 2021

The 2030 Agenda for Sustainable Development promotes sustainable global prosperity by encouraging the coordination of social, economic, and environmental policies and good governance reforms. Cities are expected to play an essential role in implementing the 2030 Agenda. Local programs are to be implemented by multi-actor governance systems (including government agencies, businesses, nonprofits, and philanthropic organizations) that operate across multiple policy domains and provide extensive opportunities for stakeholder participation. Local program finance may require a combination of public, private, and philanthropic resources. We analyze the prospects for local implementation of the 2030 Agenda in large U.S. cities by examining local capacity to plan and carry out cross-sectoral collaborative initiatives. We review sustainability planning in the cities that participated in the Sustainable Development Solutions Network planning demonstration. We analyze an inventory of urban revitalization initiatives to assess local capacity to carry out collaborations. We show that local capacity is associated with having an active local environmental agenda and making progress toward achieving sustainable development goals. However, local capacity appears to be concentrated in larger cities. Although the demands on local governance are daunting, our examination of local capacity to plan and execute cross-sectoral collaborative initiatives in large U.S. cities creates guarded optimism.
On the Primary Influences of Age on Articulation and Phonation in Maximum Performance Tasks
Authors: Karlsson Fredrik , Hartelius Lena
Published: October 2021

Maximum performance tasks have been identified as possible domains where incipient signs of neurological disease may be detected in simple speech and voice samples. However, it is likely that these will simultaneously be influenced by the age and sex of the speaker. In this study, a comprehensive set of acoustic quantifications were collected from the literature and applied to productions of sustained [a] productions and Alternating Motion Rate diadochokinetic (DDK) syllable sequences made by 130 (62 women, 68 men) healthy speakers, aged 20–90 years. The participants were asked to produce as stable (sustained [a] and DDK) and fast (DDK) productions as possible. The full set of features were reduced to a functional subset that most efficiently modeled sex-specific differences between younger and older speakers using a cross-validation procedure. Twelve measures of [a] and 16 measures of DDK sequences were identified across men and women and investigated in terms of how they were altered with increasing age of speakers. Increased production instability is observed in both tasks, primarily above the age of 60 years. DDK sequences were slower in older speakers, but also altered in their syllable and segment level acoustic properties. Increasing age does not appear to affect phonation or articulation uniformly, and men and women are affected differently in most quantifications investigated.
Chronic Lymphocytic Inflammation with Pontine Perivascular Enhancement Responsive to Steroids May Extend above and below Pons and Is Associated with Other Autoimmune Diseases
Authors: Berry Brent , Joppa Stephanie , Labin Edward , Puram Vikram , McCleary Kaci , Clark H. Brent , Nelson Flavia
Published: October 2021

Many autoimmune diseases can affect the central nervous system, and their varying clinical presentations often confound a straightforward diagnosis. In this report, we describe a unique presentation of CLIPPERS syndrome. To our knowledge, this is the first case to demonstrate significant supratentorial involvement with symmetric and non-confluent lesions in the medial orbitofrontal cortex; additionally, this is the second case to describe an association between diagnoses of hypothyroidism and CLIPPERS.
Hierarchical Structural Analysis Method for Complex Equation-Oriented Models
Authors: Wang Chao , Wan Li , Xiong Tifan , Xie Yuanlong , Wang Shuting , Ding Jianwan , Chen Liping
Published: October 2021

Structural analysis is a method for verifying equation-oriented models in the design of industrial systems. Existing structural analysis methods need flattening of the hierarchical models into an equation system for analysis. However, the large-scale equations in complex models make structural analysis difficult. Aimed to address the issue, this study proposes a hierarchical structural analysis method by exploring the relationship between the singularities of the hierarchical equation-oriented model and its components. This method obtains the singularity of a hierarchical equation-oriented model by analyzing a dummy model constructed with the parts from the decomposing results of its components. Based on this, the structural singularity of a complex model can be obtained by layer-by-layer analysis according to their natural hierarchy. The hierarchical structural analysis method can reduce the equation scale in each analysis and achieve efficient structural analysis of very complex models. This method can be adaptively applied to nonlinear-algebraic and differential-algebraic equation models. The main algorithms, application cases and comparison with the existing methods are present in this paper. The complexity analysis results show the enhanced efficiency of the proposed method in the structural analysis of complex equation-oriented models. Compared with the existing methods, the time complexity of the proposed method is improved significantly.
On a Boundary Value Problem of Hybrid Functional Differential Inclusion with Nonlocal Integral Condition
Authors: El-Sayed Ahmed M. A. , El-Sayed Wagdy G. , S. Amrajaa Somyya
Published: October 2021

In this work, we present a boundary value problem of hybrid functional differential inclusion with nonlocal condition. The boundary conditions of integral and infinite points will be deduced. The existence of solutions and its maximal and minimal will be proved. A sufficient condition for uniqueness of the solution is given. The continuous dependence of the unique solution will be studied.