bluuebunny/crossref_metadata_2025 huggingface dataset:
Using Human-Centered Design in Community-Based Public Health Research: Insights from the ECHO Study on COVID-19 Vaccine Hesitancy in Montreal, Canada
Authors: Abalovi Krystelle Marie , Fortin Geneviève , Parvez Maryam , Senga Joyeuse , Abou-Malhab Joe , Tuong-Nguyen Cat , Quach Caroline , Vandermorris Ashley , Zinzser Kate , McKinnon Britt
Published: January 2025

(1) Background: This study used human-centered design (HCD) within a community-based research project to collaboratively develop local strategies aimed at enhancing COVID-19 vaccine confidence among children and youth. (2) Methods: HCD projects were carried out between December 2021 and August 2022 by four community-based design (CBD) teams in Montreal, Canada. The CBD teams were composed of parent and youth community members, public health and social science researchers, and HCD specialists. Process evaluation data, collected from the CBD team members through focus group discussions and written questionnaires, were used to reflect on the use of HCD in this project. (3) Results: The CBD teams designed and implemented projects addressing factors they identified as contributing to COVID-19 vaccine hesitancy for children and youth in their communities, including misinformation, lack of trust, social inequities, and resistance to pandemic-related restrictions. The CBD team members appreciated many aspects of the HCD approach, especially the values it stands for, such as empathy, co-creation, and collaboration. HCD and public health specialists described some tension between the different disciplinary approaches. (4) Conclusions: HCD holds promise for addressing complex public health issues, though further exploration of strategies for integrating HCD within established models of community-based public health research is needed.
Racial and Economic Disparities in High-Temperature Exposure in Brazil
Authors: da Silva Hosana Gomes , Requia Weeberb J.
Published: January 2025

Primary studies analyzing the distribution of exposure to the consequences of climate change among different vulnerable groups are scarce. This study addresses this gap by investigating racial and economic disparities in high-temperature exposure in Brazil, focusing on the impact on vulnerable subpopulations. We utilized georeferenced temperature data from the Global High-Resolution Estimates of Extreme Heat (GEHE) and population data from the 2010 Census. The disparity analyses included (i) estimating the exposure rate to temperatures exceeding 28 °C, expressed as population-weighted heat exposure (PHE¯); (ii) determining the difference in exposure between the most and least exposed groups; and (iii) calculating weighted Gini coefficients. The findings reveal that low-income and black, brown, and indigenous populations are predominantly the most exposed to PHE¯ exceeding 28 °C. Nationally, the indigenous population is the most exposed racial group, with a PHE¯ 47% higher than that of the white population. Stratified analyses indicate that, despite varying climatic and environmental conditions across regions, the black-brown-indigenous population consistently faces the highest heat exposure in Brazil. Income disparity analyses show that the lowest per capita income groups are the most exposed to high temperatures across the country. The study highlights the impact of climate change on economic inequality and the deepening of within-country inequalities, particularly affecting socioeconomically disadvantaged groups. These findings underscore the urgent need for evidence-informed public policies to address racial and economic disparities in high-temperature exposure, mitigate health risks associated with climate change, and emphasize the importance of context-sensitive analyses for a comprehensive understanding of heat-related risks and public health.
Impact of Physical Activity on Health Behavior Change and Mental Health During the COVID-19 Epidemic Among Chinese Adults: China Health and Retirement Longitudinal Study (CHARLS)
Authors: Yin Wupeng , Sifre-Acosta Niliarys , Chamorro Daisy , Chowdhury Susmita , Hu Nan
Published: January 2025

Background: The COVID-19 pandemic caused significant disruptions to daily life, affecting regular physical activity (PA) and health behaviors worldwide. This study investigates the associations between PA domains and changes in health behaviors and mental health outcomes among middle-aged and old Chinese adults. Methods: Using wave 5 cross-sectional data from the 2020 China Health and Retirement Longitudinal Study, we analyzed 17,180 adults aged 45 and above, focusing on health behavior changes such as smoking, alcohol consumption, dietary adjustments, and panic purchasing, as well as mental health outcomes like anxiety and fear. PA was classified by intensity levels—light, moderate, and vigorous—and by activity purposes—total, leisure, and occupational. Results: The findings indicate that leisure PA is associated with healthier behaviors, including lower odds of increased smoking (OR = 0.71, 95% CI: 0.57–0.90) and alcohol consumption (OR = 0.70, 95% CI: 0.54–0.90), whereas occupational PA is linked to adverse behavioral outcomes, such as higher odds of smoking (OR = 1.45, 95% CI: 1.15–1.83) and alcohol use (OR = 1.43, 95% CI: 1.10–1.86). Additionally, participants engaged in all domains of PA were more likely to experience anxiety and fear compared to those who were physically inactive. Conclusions: Our limited understanding of the role PA has on behavioral and mental health during public health crises highlights the importance of having tailored strategies to enhance resilience in similar future scenarios.
Integrating Green Care Initiatives into Conventional Health Systems: Which Governance Dimensions Can Guide This Process?
Authors: Rigo Alessandra , Pisani Elena , Secco Laura
Published: January 2025

Green Care initiatives (GCIs) encompass various interventions that support physical, mental, and social well-being through interaction with nature. Integrating GCIs into conventional healthcare systems is a complex task that requires multi-actor and multi-level governance efforts. This study examines, through a systematized literature review, the relevant governance dimensions to facilitate the inclusion of GCIs in traditional care pathways. From the analysis of the 36 selected studies, four key dimensions were identified: organizational structure, knowledge, legitimacy, and decentralization. The analysis highlights the need to strengthen the responsibility of Green Care actors as healthcare service providers, enhance local authorities’ role in creating new integrated service delivery networks, combine different knowledge perspectives, and legitimize non-healthcare actors. Recommendations are made to address these governance aspects to facilitate the integration of GCIs and channel their benefits in prevention and health promotion. Adopting an adequate governance framework is fundamental for mainstreaming GCIs in current healthcare systems.
A Blockchain Copyright Protection Model Based on Vector Map Unique Identification
Authors: Wang Heyan , Tang Nannan , Zhu Changqing , Ren Na , Wang Changhong
Published: January 2025

Combining blockchain technology with digital watermarking presents an efficient solution for safeguarding vector map files. However, the large data volume and stringent confidentiality requirements of vector maps pose significant challenges for direct registration on blockchain platforms. To overcome these limitations, this paper proposes a blockchain-based copyright protection model utilizing unique identifiers (BCPM-UI). The model employs a distance ratio-based quantization watermarking algorithm to embed watermark information into vector maps and then generates unique identifiers based on their topological and geometric parameters. These identifiers, rather than the vector maps themselves, are securely registered on the blockchain. To ensure reliable copyright verification, a bit error rate (BER)-based matching algorithm is introduced, enabling accurate comparison between the unique identifiers of suspected infringing data and those stored on the blockchain. Experimental results validate the model’s effectiveness, demonstrating the high uniqueness and robustness of the identifiers generated. Additionally, the proposed approach reduces blockchain storage requirements for map data by a factor of 200, thereby meeting confidentiality standards while maintaining practical applicability in terms of copyright protection for vector maps.
Effects of Cell Seeding Density, Extracellular Matrix Composition, and Geometry on Yes-Associated Protein Translocation in Corneal Fibroblasts
Authors: Subramanian Divya , Tjahjono Nathaniel S. , Nammi Satweka , Miron-Mendoza Miguel , Varner Victor D. , Petroll W. Matthew , Schmidtke David W.
Published: January 2025

Corneal fibroblasts are central to normal and abnormal wound healing in the cornea. During the wound healing process, several biochemical and biophysical signals that are present in the extracellular matrix (ECM) play critical roles in regulating corneal fibroblast behavior. The translocation and activation of Yes-associated protein (YAP)—a main transcriptional factor in the Hippo signaling pathway—is one example of mechanotransduction involving these signals. However, how corneal fibroblasts integrate these simultaneous cues is unknown. In this study, we utilized well-defined micropatterns of aligned collagen fibrils and other ECM proteins to explore the effects of cell density, topography, geometric confinement, and ECM composition on the translocation of YAP in corneal fibroblasts. We observed that when human corneal fibroblasts (HTKs) were confined to narrow micropatterns (50 μm and 100 μm) of proteins, there was a high degree of cell alignment irrespective of cell seeding density. However, the location of YAP was dependent upon the cell seeding density, ECM composition, and topography. YAP was more nuclear-localized on substrates coated with aligned collagen fibrils or fibronectin as compared to substrates coated with monomeric collagen, random collagen fibrils, or poly-L-Lysine. In addition, we also observed that YAP nuclear localization was significantly reduced when HTKs were cultured on aligned collagen fibrils, monomeric collagen, or fibronectin in the presence of monoclonal blocking antibodies against α5 or β1 integrin subunits. Finally, we observed that HTK cells formed fibrillar fibronectin on both monomeric collagen and aligned collagen fibrils. These findings provide new insights into how simultaneous biochemical and biophysical cues affect YAP localization in corneal fibroblasts.
Design and Expression of Fasciola hepatica Multiepitope Constructs Using mRNA Vaccine Technology
Authors: Sánchez-Montejo Javier , Strilets Tania , Manzano-Román Raúl , López-Abán Julio , García-Blanco Mariano A. , Vicente Belén , Muro Antonio
Published: January 2025

Fasciola hepatica is a parasitic trematode responsible for fascioliasis, a significant zoonotic disease affecting livestock worldwide, as well as humans. This study identifies peptides with potential for use in vaccines against Fasciola hepatica and validates multi-epitope constructs from those peptides in vitro. Putative protein sequences derived from the genome of F. hepatica were integrated with phase-specific transcriptomic data to prioritize highly expressed proteins. Among these, extracellular proteins were selected using DeepLoc 2.0 and strong binding affinities across diverse human and murine alleles were predicted with the IEDB MHC II tool. Peptides were further selected based on their toxicity, immunogenicity, and allergenicity. Finally, 55 high-priority candidates were obtained. To express these candidates, mRNA constructs encoding various combinations of these peptides were designed, synthesized using in vitro transcription with T7 or SP6 RNA polymerases, and transfected into cells for expression analysis. SP6 polymerase produced proper capping using CleanCapAG and was far superior in transcribing peptide constructs. Peptides fused in frame with eGFP were expressed efficiently, particularly when peptides were positioned at the 3′ terminus, opening a new field of peptide vaccines created using mRNA technology.
Oxidative High Mobility Group Box-1 Accelerates Mitochondrial Transfer from Mesenchymal Stem Cells to Colorectal Cancer Cells Providing Cancer Cell Stemness
Authors: Sasaki Rika , Luo Yi , Kishi Shingo , Ogata Ruiko , Nishiguchi Yukiko , Sasaki Takamitsu , Ohmori Hitoshi , Fujiwara-Tani Rina , Kuniyasu Hiroki
Published: January 2025

Mitochondria are important organelles for cell metabolism and tissue survival. Their cell-to-cell transfer is important for the fate of recipient cells. Recently, bone marrow mesenchymal stem cells (BM-MSCs) have been reported to provide mitochondria to cancer cells and rescue mitochondrial dysfunction in cancer cells. However, the details of the mechanism have not yet been fully elucidated. In this study, we investigated the humoral factors inducing mitochondrial transfer (MT) and the mechanisms. BM-MSCs produced MT in colorectal cancer (CRC) cells damaged by 5-fluorouracil (5-FU), but were suppressed by the anti-high mobility group box-1 (HMGB1) antibody. BM-MSCs treated with oxidized HMGB1 had increased expression of MT-associated genes, whereas reduced HMGB1 did not. Inhibition of nuclear factor–κB, a downstream factor of HMGB1 signaling, significantly decreased MT-associated gene expression. CRC cells showed increased stemness and decreased 5-FU sensitivity in correlation with MT levels. In a mouse subcutaneous tumor model of CRC, 5-FU sensitivity decreased and stemness increased by the MT from host mouse BM-MSCs. These results suggest that oxidized HMGB1 induces MTs from MSCs to CRC cells and promotes cancer cell stemness. Targeting of oxidized HMGB1 may attenuate stemness of CRCs.
Elucidating the Mechanism of VVTT Infection Through Machine Learning and Transcriptome Analysis
Authors: Chen Zhili , Jiang Yongxin , Cui Jiazhen , Li Wannan , Han Weiwei , Liu Gang
Published: January 2025

The vaccinia virus (VV) is extensively utilized as a vaccine vector in the treatment of various infectious diseases, cardiovascular diseases, immunodeficiencies, and cancers. The vaccinia virus Tiantan strain (VVTT) has been instrumental as an irreplaceable vaccine strain in the eradication of smallpox in China; however, it still presents significant adverse toxic effects. After the WHO recommended that routine smallpox vaccination be discontinued, the Chinese government stopped the national smallpox vaccination program in 1981. The outbreak of monkeypox in 2022 has focused people’s attention on the Orthopoxvirus. However, there are limited reports on the safety and toxic side effects of VVTT. In this study, we employed a combination of transcriptomic analysis and machine learning-based feature selection to identify key genes implicated in the VVTT infection process. We utilized four machine learning algorithms, including random forest (RF), minimum redundancy maximum relevance (MRMR), eXtreme Gradient Boosting (XGB), and least absolute shrinkage and selection operator cross-validation (LASSOCV), for feature selection. Among these, XGB was found to be the most effective and was used for further screening, resulting in an optimal model with an ROC curve of 0.98. Our analysis revealed the involvement of pathways such as spinocerebellar ataxia and the p53 signaling pathway. Additionally, we identified three critical targets during VVTT infection—ARC, JUNB, and EGR2—and further validated these targets using qPCR. Our research elucidates the mechanism by which VVTT infects cells, enhancing our understanding of the smallpox vaccine. This knowledge not only facilitates the development of new and more effective vaccines but also contributes to a deeper comprehension of viral pathogenesis. By advancing our understanding of the molecular mechanisms underlying VVTT infection, this study lays the foundation for the further development of VVTT. Such insights are crucial for strengthening global health security and ensuring a resilient response to future pandemics.
Simulation-Driven Optimization of Thermochemical Energy Storage in SrCl2-Based System for Integration with Solar Energy Technology
Authors: Korkua Suratsavadee K. , Thubsuang Uthen , Sakphrom Siraporn , Dash Santanu Kumar , Tesanu Chalearm , Thinsurat Kamon
Published: January 2025

This study presents a simulation-driven optimization of thermochemical energy storage using SrCl2-based systems for integration with solar energy technologies. Thermochemical sorption systems offer promising potential for enhancing solar energy-storage efficiency by capturing both thermal and electrical energy. However, optimizing sorption processes remains crucial for maximizing energy storage capacity. This work utilized advanced simulation tools to analyze the heat and mass transfer dynamics within SrCl2-EG composites and evaluate system performance under varying compression ratios (CR), reactant temperatures, and heat transfer fluid (HTF) flow rates. The results demonstrate that adjusting CR enhances the overall system efficiency. CR = 4 yields the highest desorption rate of 93.8%, while reducing the required HTF mass flow by nearly tenfold compared to lower CR values. Higher CR contributes to a reduction in sensible heat loss, allowing a greater percentage of thermal energy to be captured. Simulations also show that optimized SrCl2-based systems can integrate effectively with solar energy conversion technologies, making them highly suitable for both energy storage and cooling applications. This research underscores the role of thermochemical energy storage systems in providing more sustainable and efficient solar energy solutions. By reducing energy losses and improving the reliability of the energy storage process, SrCl2-based systems offer significant advantages for renewable energy integration.
Training for Radiographic Assessment of Scoliosis: A Pilot Educational Study
Authors: Filippetti Mirko , Tomasello Sofia , Di Censo Rita , Modenese Angela , Scaturro Dalila , Letizia Mauro Giulia , Varalta Valentina , Smania Nicola , Picelli Alessandro
Published: January 2025

Background: Given the prevalence of scoliosis, it is essential for residents in Physical and Rehabilitation Medicine to develop proficiency in evaluating spinal radiographs, particularly in measuring the Cobb angle. This education and training study aimed to define the minimum educational requirements for residents to achieve competency in Cobb angle measurement with acceptable inter- and intra-operator variability, as reported in the literature. Methods: In this ethics-approved study, Physical and Rehabilitation Medicine residents measured Cobb angles and the Risser index using specialized software on 30 different spinal X-rays, with oversight to ensure consistency and eliminate bias. Results: The data revealed that 44.4% of residents achieved the target accuracy for primary Cobb angles (<3.6°), but only 11.1% did so for secondary curves. For intra-operator error, 88.9% of residents met the target for primary curves, but only 11.1% achieved the target for secondary curves. The Risser index showed minimal deviation across all participants. Conclusions: These findings highlight that mastering Cobb angle measurement is challenging and generally requires more than 20 different measurements for inter-operator reliability and over 30 repetitions for intra-operator reliability, particularly when secondary curves are included.
The Limitation of HLA Diversity as a Risk Factor for Pediatric-Onset Autoimmune Rheumatic Disease
Authors: Kalampokis Ioannis , Wong Craig S. , Ma Jihyun , Smith Lynette M. , Masten Barbara J. , Chabot-Richards Devon , Pisetsky David S.
Published: January 2025

Background: HLA homozygosity of specific alleles at a single locus is associated with increased risk for autoimmunity and/or more severe clinical phenotypes. However, the contribution of the overall limitation of HLA diversity across multiple loci to autoimmunity risk remains to be determined. Methods: We conducted a proof-of-concept case–control study of 413 individuals (279 cases with pediatric-onset autoimmune rheumatic diseases, 134 matched controls) examining the “Limitation of HLA Diversity” (LoHLAD) across multiple loci as an allele-independent risk factor for autoimmunity. We examined the association of LoHLAD with pediatric-onset autoimmune rheumatic diseases at five HLA loci (A, B, DQB1, DRB1, DRB3/4/5). LoHLAD was defined as (1) homozygosity at any of the examined loci, and/or (2) the presence of a single allele or the complete lack of an allele at the HLA-DRB3/4/5 locus. Results: The frequency of LoHLAD at any locus was significantly higher in cases compared to controls (65.95% vs. 30.60%, OR 4.39 [2.82–6.84], p < 0.0001). Higher frequencies of LoHLAD in cases compared to controls were observed at both class I (19.35% vs. 10.45%, OR 2.06 [1.10–3.86], p = 0.031) and class II (54.48% vs. 20.15%, OR 4.74 [2.92–7.69], p < 0.0001) loci. Specifically, significant differences between cases and controls were observed at the B (OR 8.63 [1.14–65.55], p = 0.016), DQB1 (OR 3.34 [1.27–8.78], p = 0.016), and DRB3/4/5 (OR 4.64 [2.77–7.75], p < 0.0001) loci. Multiple logistic regression models confirmed the ability of LoHLAD to positively predict autoimmunity. Conclusions: LoHLAD is a significant allele-independent risk factor for pediatric-onset autoimmune rheumatic disease.
Effects of Priming with Light vs. Heavy Loads on Weightlifting Performance
Authors: Stavropoulos Theodoros , Zaras Nikolaos , Kelekian Georgia-Kassandra , Mpampoulis Thomas , Avloniti Alexandra , Chatzinikolaou Athanasios , Terzis Gerasimos
Published: January 2025

Background/Objectives: The purpose of the study was to investigate the effects of a priming training session with either a light or heavy load snatch and clean pulls on weightlifting performance. Methods: Twelve well-trained weightlifters (seven males and five females) participated in the study. The athletes followed a counterbalanced study design comparing three treatments, including a day of rest (control) and two priming sessions involving two different weightlifting derivatives—the snatch and the clean pulls—which were performed either with 80% of the one-repetition maximum (1-RM) (LP) or with 110% of the 1-RM (HP). Twenty-four hours later, the 1-RM strength test for the snatch and clean and jerk, as well as the barbell kinematic characteristics at 100% of the 1-RM in the snatch and clean and jerk, were measured. The rate of perceived exertion (RPE) was measured following the priming sessions. Results: Performance in snatch remained unchanged following the LP and HP. However, performance in the clean and jerk increased significantly by 3.1% following the HP compared to the control. No significant differences were observed in barbell kinematics. The RPE was significantly higher for HP compared to LP. Conclusions: These results suggest that an HP performed 24 h prior to the 1-RM evaluation in weightlifting may have significantly increased performance in the clean and jerk. These changes may not be explained by barbell kinematics.
Plasma Dielectric Etching with C4H2F6 Isomers of Low Global-Warming Potential
Authors: Choi Minsu , Lee Youngseok , Cho Chulhee , Jeong Wonnyoung , Seong Inho , Haque Jami Md Ehsanul , Choi Byeongyeop , Seo Seonghyun , Kim Sijun , You Shinjae , Yeom Geun Young
Published: January 2025

This paper presents the observed changes when replacing the widely used CHF3 gas in semiconductor processing with two isomeric gases, C4H2F6-iso and C4H2F6-Z. This study investigates the etching process results of SiO2 and Si3N4 by varying the ratios of CHF3, C4H2F6 gases. The process outcomes were analyzed using ellipsometer and X-ray photoelectron spectroscopy, while plasma radical densities were examined through quadrupole mass spectrometry. The results are compared across five conditions, with substitution gas ratios ranging from 0% to 100%. The process results indicated that the selectivity increased at certain gas ratios. The diagnostic results provided the ratios of various etchants within the plasma. This research advances the development of alternative precursors designed to mitigate the effects of global warming.
Does Information Asymmetry Affect Firm Disclosure? Evidence from Mergers and Acquisitions of Financial Institutions
Authors: Chen Bing , Chen Wei , Yang Xiaohui
Published: January 2025

We use a quasi-exogenous shock to information asymmetry among shareholders to evaluate the effect of information asymmetry on corporate disclosure. In the post-Regulation Fair Disclosure (FD) period, the merger between a shareholder and a lender of the same firm provides a shock to the information asymmetry among equity investors, because Regulation FD applies to shareholders but not lenders. After the merger, the shareholder gains access to the firm-specific private information held by the lender, which produces an asymmetry in the information held by shareholders. We first provide evidence that information asymmetry among shareholders indeed increases after the shareholder–lender mergers. We then use a difference-in-differences research design to show that after shareholder–lender merger transactions, firms issue more quarterly forecasts (including earnings, sales, capital expenditure, earnings before interest, taxes, amortization (EBITDA), and gross margin), and the quarterly earnings forecasts are more precise. This study provides direct empirical evidence that information asymmetry among shareholders affects corporate disclosure. Firms can address increased information asymmetry by providing more disclosures, fostering a more equitable information environment. Additionally, policymakers might consider these results when evaluating the implications of Regulation FD, particularly in the context of mergers and acquisitions (M&A) of financial institutions where a shareholder gains access to private information held by a debt holder.
Whistleblowing Disclosure as a Shield Against Earnings Management: Evidence from the Insurance Sector
Authors: Belgacem Ines
Published: January 2025

One of the fundamental components of internal controls, a whistleblowing system (WBS) is crucial for preventing fraud, addressing irregularities, and enhancing good governance. The purpose of this study is to investigate the impact of whistleblower disclosures on earnings management in Saudi Arabia’s Takaful Insurance (TKI) sector between 2017 and 2023. To this end, a whistleblowing index was constructed as a tool to evaluate the whistleblowing framework’s effectiveness. Using the Dynamic Generalized Method of Moments (GMM) to account for endogeneity, it was found that most Saudi insurance companies increased their efforts to disclose information about whistleblowers, which significantly reduced earnings management practices. Specifically, the study concludes that the size of the audit committee (ACS) significantly and negatively affects how insurance businesses manage their earnings when a whistleblower system is in place. Additionally, there is a notable and adverse effect on earnings management from board size (BSZ), the percentage of non-executive independent members (PNIM), and Shariah board size (SBS). However, it was found that earnings management is unaffected by the frequency of board meetings (BMFR). This study adds to the body of knowledge by demonstrating how corporate governance enhances the effectiveness of the whistleblowing system.
Optimization of Crop Yield in Precision Agriculture Using WSNs, Remote Sensing, and Atmospheric Simulation Models for Real-Time Environmental Monitoring
Authors: Barrile Vincenzo , Maesano Clemente , Genovese Emanuela
Published: January 2025

Due to the increasing demand for agricultural production and the depletion of natural resources, the rational and efficient use of resources in agriculture becomes essential. Thus, Agriculture 4.0 or precision agriculture (PA) was born, which leverages advanced technologies such as Geographic Information Systems (GIS), Artificial Intelligence (AI), sensors and remote sensing techniques to optimize agricultural practices. This study focuses on an innovative approach integrating data from different sources, within a GIS platform, including data from an experimental atmospheric simulator and from a wireless sensor network, to identify the most suitable areas for future crops. In addition, we also calculate the optimal path of a drone for crop monitoring and for a farm machine for agricultural operations, improving efficiency and sustainability in relation to agricultural practices and applications. Expected and obtained results of the conducted study in a specific area of Reggio Calabria (Italy) include increased accuracy in agricultural planning, reduced resource and pesticide use, as well as increased yields and more sustainable management of natural resources.
Quantifying Evapotranspiration and Environmental Factors in the Abandoned Saline Farmland Using Landsat Archives
Authors: Zhao Liya , Wu Jingwei , Yang Qi , Zhao Hang , Mao Jun , Yu Ziyang , Liu Yanqi , Gobin Anne
Published: January 2025

This study investigates the complex interaction of biophysical and meteorological factors that drive evapotranspiration (ET) in saline environments. Leveraging a total of 182 cloud-free Landsat 5/8 time-series data from 1988 to 2019, we employed the Surface Energy Balance System (SEBS) model to quantify ET and investigate its relationships with soil salinity, vegetation cover, groundwater depth, and landscape metrics. We validated the predicted ET at two experimental sites using ET observation calculated by a water balance model. The result shows an R2 of 0.78 and RMSE of 0.91 mm for the SEBS predicted ET, indicating high accuracy of the ET estimation. We detected abandoned saline farmland patches across Hetao and extracted the normalized difference vegetation index (NDVI), salinization index (SI), and the predicted ET for analysis. The results indicate that ET is negatively correlated with SI with a Pearson correlation coefficient (r) up to −0.7, while ET is positively correlated with NDVI (r = 0.4). In addition, we designed a control-variable experiment in the Yichang subdistrict to investigate the effects of groundwater depth, land aggregation index, soil salinity index, and the area of abandoned saline farmland patches on ET. The results indicate that increased NDVI could significantly enhance ET, while smaller saline farmland patches exhibited greater sensitivity to groundwater recharge, with higher averaged ET than larger patches. Moreover, we analyzed factor importance using Lasso regression and Random Forest (RF) regression. The result shows that the ranking of the importance of the features is consistent for both methods and for all the features, with NDVI being the most important (with an RF importance score of 0.4), followed by groundwater table depth (GWTD), and the influence of the surface area of abandoned saline farmland being the weakest. We found that smaller patches of abandoned saline farmland were more sensitive to changes in groundwater levels induced by nearby irrigation, affecting their averaged ET more dynamically than larger patches. Decreasing patch size over time indicates ongoing changes in land management and ecological conditions. This study, through a multifactor analysis of ET in abandoned saline farmland and its intrinsic factors, provides a reference for evaluating the dry drainage efficiency of abandoned saline farmland in a dry drainage system.
Spatiotemporal Coupling of New-Type Urbanization and Ecosystem Services in the Huaihe River Basin, China: Heterogeneity and Regulatory Strategy
Authors: Huang Muyi , Guo Qin , Zhang Guozhao , Tang Yuru , Wu Xue
Published: January 2025

Strengthening the exploration of synergistic promotion mechanisms between ecosystem services (ESs) and new urbanization is of great significance for watershed development. In this work, we revealed the evolution mechanism of coupling coordination development degree (CCD) between ESs and new urbanization and its driving factors in the Huaihe River Basin (HRB) from 1980 to 2020 using a combination of the CCD model, Exploratory Spatial Data Analysis (ESDA) method, and GeoDetector model. Additionally, we employed the PLUS model to investigate multi-scenario simulations. The results demonstrate that ESs showed a decline initially, followed by an increase, while the urbanization index showed consistent annual growth over the four decades. Furthermore, the CCD between the ESs and urbanization showed a yearly optimization trend. The CCD demonstrated notable spatial clustering characteristics, with factors such as precipitation, distance from water body, elevation, and per area GDP emerged as the primary drivers. Under scenarios of ecological protection, comprehensive development, and natural protection, the value of ESs from 2020 to 2050 maintained an upward trend; however, it fell with the decrease under the scenario of cropland protection. These research findings offer valuable decision-making support for the differentiated regulation of ecosystem functions and promotion of high-quality urbanization development in the HRB.
An Insight into Testicular Macrocalcification—A Retrospective Study of 42 Cases on a Rare Sonographic Finding
Authors: Pedersen Malene Roland Vils , Toft Ditte Marie , Lindebjerg Jan , Rafaelsen Søren Rafael , Lildal Søren Kissow
Published: January 2025

A single testicular microlithiasis is a common finding during sonography, while macrocalcification is a rare and incidental finding. The literature on macrocalcification is limited. Typically, testicular calcifications, whether microscopic or macroscopic, are benign but they can have a clinical significance. This multicenter study aimed to investigate the symptoms and prevalence of testicular cancer in patients with macrocalcification. Methods: Testicular ultrasound examination reports from four hospitals’ PACS database, covering the period 2014–2023, were screened for diagnoses of macrocalcification. Inclusion criteria required that the radiology report described macrocalcification supported by ultrasound images. Results: Macrocalcifications were identified in 42 male patients, with a mean age of 45 years. Sixteen macrocalcifications were in the right testicle, twenty in the left, and six were bilateral. Microlithiasis were found in 22 patients (52.4%), with 11 (26.2%) bilateral, 3 (7.1%) left-sided, and 8 (19.1%) right-sided. Testicular tumors were found in six patients. Conclusion: Testicular macrocalcification exhibited large visual variation and diverse clinical history. However, we found a low number of patients with testicular macrocalcification and testicular tumors, indicating that macrocalcifications have a benign nature, and that macrocalcification alone should not be a primary concern for malignancy, but this needs to be confirmed in further studies.
Enterobacter soli Strain AF-22b-4245: Study of the Genome and the Effect on Wheat Growth
Authors: Sokolova Ekaterina Alexeevna , Mishukova Olga Viktorovna , Khlistun Inna Viktorovna , Tromenschleger Irina Nikolaevna , Chumanova Evgeniya Vladimirovna , Voronina Elena Nikolaevna
Published: January 2025

Background: In this work the plant growth-promoting (PGP) qualities of the Enterobacter soli strain AF-22b-4245 were studied, including screening tests for PGP, whole genome sequencing (WGS) and genome annotation, and greenhouse experiments on wheat. A gene table was formed that allows us to evaluate the potential PGP properties of a microorganism based on the results of genome-wide sequencing. Results: Based on the results of screening tests and genome annotation, it can be concluded that the E. soli strain AF-22b-4245 strain may have PGP properties, which consist in the ability to survive in arid and saline soils contaminated with copper, arsenic, lead, and chromium soils, form biofilms, produce phytohormones, siderophores, and solubilize phosphorus. Based on the results of experiments on wheat, the E. soli strain AF-22b-4245 increases the efficiency of mineral fertilizers; this effect persists even in conditions of drought and excess salt. It has been shown that E. soli A F22b-4245 can compensate for the lack of soluble phosphorus in the mineral fertilizer, probably by solubilizing insoluble forms in the soil.
Triazole-Estradiol Analogs Induce Apoptosis and Inhibit EGFR and Its Downstream Pathways in Triple Negative Breast Cancer
Authors: Acheampong Felix , Ostlund Trevor , Hedge Emily , Laddusaw Jacqueline , Alotaibi Faez , Elshaier Yaseen A. M. M. , Halaweish Fathi
Published: January 2025

Triple negative breast cancer, TNBC, is a difficult disease to treat due to relapse and resistance to known therapies. Epidermal growth factor receptor (EGFR), a tyrosine kinase responsible for downstream signaling leading to cell growth and survival, is typically overexpressed in TNBC. Our previous work has detailed the synthesis of triazole-estradiol derivatives as inhibitors of EGFR and downstream receptors, and this work continues that discussion by evaluating them in EGFR-dependent TNBC cell models MDA-MB-231 and MDA-MB-468. Compound Fz25 was cytotoxic against both MDA-MB-231 and MDA-MB-468 cell lines, yielding IC50 values of 8.12 ± 0.85 and 25.43 ± 3.68 µM, respectively. However, compounds Fz57 and Fz200 were potent against only MDA-MB-231 cells, generating IC50 values of 21.18 ± 0.23 and 10.86 ± 0.69 µM, respectively. Pathway analyses revealed that Fz25, Fz57 and Fz200 arrested the G0/G1 phase of the cell cycle and concomitantly suppressed cell cycle regulators, cyclin D1, cyclin E and Dyrk1B in MDA-MB-231 cells. Additionally, all compounds inhibited EGFR and its downstream signaling pathways—extracellular receptor kinase (ERK) and the mammalian target of rapamycin (mTOR)—in a dose-dependent manner. Furthermore, Fz25, Fz57 and Fz200 induced apoptosis in MDA-MB-231 cells by modulating morphological changes, including chromatin condensation, and attenuating the levels of cytochrome c, APAF1, caspases-3 and -9 as well as cleaved PARP. Of these compounds, only Fz25 showed overall satisfactory ADMET properties in silico. Similarly, Fz25 showed suitable binding parameters explored using molecular dynamic simulations in silico. These findings suggest that Fz25 warrants further preclinical and clinical investigations as a new generation of triazole congeners with significant potency in EFGR-dependent TNBC.
Molecular Modification of Queen Bee Acid and 10-Hydroxydecanoic Acid with Specific Tripeptides: Rational Design, Organic Synthesis, and Assessment for Prohealing and Antimicrobial Hydrogel Properties
Authors: Hong Song , Baravkar Sachin B. , Lu Yan , Masoud Abdul-Razak , Zhao Qi , Zhou Weilie
Published: January 2025

Royal jelly and medical grade honey are traditionally used in treating wounds and infections, although their effectiveness is often variable and insufficient. To overcome their limitations, we created novel amphiphiles by modifying the main reparative and antimicrobial components, queen bee acid (hda) and 10-hydroxyl-decanoic acid (hdaa), through peptide bonding with specific tripeptides. Our molecular design incorporated amphiphile targets as being biocompatible in wound healing, biodegradable, non-toxic, hydrogelable, prohealing, and antimicrobial. The amphiphilic molecules were designed in a hda(hdaa)-aa1-aa2-aa3 structural model with rational selection criteria for each moiety, prepared via Rink/Fmoc-tBu-based solid-phase peptide synthesis, and structurally verified by NMR and LC–MS/MS. We tested several amphiphiles among those containing moieties of hda or hdaa and isoleucine–leucine–aspartate (ILD-amidated) or IL-lysine (ILK-NH2). These tests were conducted to evaluate their prohealing and antimicrobial hydrogel properties. Our observation of their hydrogelation and hydrogel-rheology showed that they can form hydrogels with stable elastic moduli and injectable shear-thinning properties, which are suitable for cell and tissue repair and regeneration. Our disc-diffusion assay demonstrated that hdaa-ILK-NH2 markedly inhibited Staphylococcus aureus. Future research is needed to comprehensively evaluate the prohealing and antimicrobial properties of these novel molecules modified from hda and hdaa with tripeptides.
Probiotics and Synbiotics Administered to Young Infants: Perceptions and Acceptability Amongst Carers and Healthcare Workers in Western Kenya
Authors: Otiti Mary Iwaret , Were Florence Achieng , Zaim Sevim , Nabwera Helen , Kariuki Simon , Allen Stephen
Published: January 2025

Background/Objectives: A contributory factor to childhood undernutrition is poor gut health occurring within the first 6–12 weeks of life despite exclusive breastfeeding. Pro/synbiotic administration may protect gut health. A qualitative study was conducted amongst mothers/carers and healthcare workers (HCWs) to explore their perceptions and the acceptability of pro/synbiotics administration in early life. Methods: This study was nested within a randomised, open, clinical trial of pro/synbiotics with 32 doses administered under supervision to infants between ages 0 and 5 months in western Kenya. Semi-structured interviews were conducted with 14 mothers/carers, 12 Peer Mothers and 7 healthcare workers (HCWs) selected by purposive critical and key informant sampling. Interviews were transcribed and analysed using a thematic coding framework. Results: The satisfaction with the pro/synbiotic administration was very high amongst all three groups. Commonly perceived benefits included protection from diseases, healthy growth of the infant and improved appetite. The main barriers were working mothers and other commitments making it difficult to stick to scheduled administration visits, adverse judgement and opinions in the community, and a lack of engagement from fathers. Insights were gained into different means of administering pro/synbiotics to young infants. Triangulation of the findings of the mothers/carers with HCWs showed that most identified motivations and challenges were similar. Conclusions: Pro/synbiotic administration was well accepted by the mothers/carers and HCWs and generally perceived to have health benefits. The administration of pro/synbiotics by the mothers/carers themselves to their infants may be feasible and overcome logistical challenges. Greater efforts to sensitise and engage fathers and communities would likely be critical for a community-based program.
The Efficacy of Cheonggukjang in Alleviating Menopausal Syndrome and Its Effects on the Gut Microbiome: A Randomized, Double-Blind Trial
Authors: Han A-Lum , Ryu Myeong-Seon , Yang Hee-Jong , Jeong Do-Youn , Choi Keum-Ha
Published: January 2025

Background/Objectives: Menopause is associated with various symptoms. Although hormone replacement therapy (HRT) is commonly used, concerns regarding its side effects have led to the development of alternative treatments. This study evaluated the potential health benefits of Cheonggukjang, a traditional Korean fermented soybean product in alleviating menopausal symptoms and improving metabolic parameters in postmenopausal women. Additionally, the effect of Cheonggukjang on the gut microbiome was assessed using stool analysis. Methods: In this randomized, double-blind clinical trial, 60 postmenopausal women were assigned to three groups: high-beneficial-microorganism content Cheonggukjang (HTC), low-beneficial-microorganism content Cheonggukjang (LTC), and commercially available Cheonggukjang (CC). Participants consumed 3.3 g of Cheonggukjang tablets daily for 8 weeks. We assessed menopausal symptom relief using the Kupperman index, metabolic parameters, and changes in the gut microbiome using stool analysis. Results: The Kupperman index scores significantly decreased across all three groups, with the HTC group showing the greatest improvement. No significant changes were observed in body mass index, weight, or lipid profiles. Blood glucose levels decreased significantly only in the HTC group. Microbiome analysis revealed an increase in beneficial bacteria in the HTC and CC groups and a decrease in harmful bacteria. The Firmicutes-to-Bacteroidetes ratio decreased in both HTC and CC groups, though this change was not significant. Conclusions: Cheonggukjang supplementation significantly alleviated menopausal symptoms, particularly in the HTC group, and improved the gut microbiota composition. These findings suggest that Cheonggukjang, particularly with its high beneficial microorganism content, may offer a promising alternative for managing menopausal symptoms and improving metabolic health in postmenopausal women.
Impact of the 2024 Noto Peninsula Earthquake on Nutritional Status in Residents of an Integrated Medical and Long-Term Care Facility: A Descriptive Study
Authors: Kokura Yoji
Published: January 2025

Background/Objectives: The dietary changes experienced by residents in long-term care facilities (LTCFs) following an earthquake are poorly understood. This study aimed to examine variations in nutritional status among residents of an Integrated Facility for Medical and Long-term Care (IFMLC), a particular type of Japanese LTCF, after the 2024 Noto Peninsula Earthquake. Methods: This descriptive study was conducted at the single IFMLC. A total of 115 residents living at the facility on 1 January 2024, at the time of the earthquake, were recruited for the study. The focus was the body weight and skeletal muscle mass changes observed before and after the earthquake. The observation period lasted for three months following the earthquake. Results: Water outage persisted for over a month, making dishwashing impossible and leading to an extended reliance on disposable dishes with limited capacity. This situation consequently reduced the variety and volume of meal options and overall energy intake meals. Residents’ body weight significantly decreased 3 months after the earthquake, and the prevalence of weight loss and skeletal muscle mass loss was particularly high in residents with normal swallowing function. To address nutritional deficiencies post-earthquake, the registered dietitian enhanced energy sufficiency through food fortification, oral nutritional supplements, and pre-prepared ready-to-hang liquid formulas. Conclusions: To prevent further weight and skeletal muscle mass reduction among IFMLC residents, providing ample water, and a disaster manual that can be used even with limited resources is essential. Furthermore, preparing for disasters by stockpiling foods and implementing strategies to enhance energy sufficiency is crucial.
Effect of Moderately High-Dose Vitamin D3 Supplementation on Mortality in Patients Hospitalized for COVID-19 Infection
Authors: Sümegi Liza Dalma , Varga Marina , Kadocsa Veronika , Szili Balázs , Stempler Márk , Lakatos Péter András , Németh Zsuzsanna , Takács István
Published: January 2025

Background: Despite a large number of published studies, the effect of vitamin D3 supplementation on mortality in hospitalized patients, as well as the recommended dose and duration of therapy, is unclear. In our retrospective study, we aimed to investigate the impact of vitamin D deficiency and moderately high-dose vitamin D3 supplementation on mortality and disease outcomes in patients with COVID-19 infection. Methods: We analyzed data from 148 COVID-19-infected hospitalized patients in two different departments, Internal Medicine and Oncology, at Semmelweis University. The severity of COVID-19 and the treatment used were the same except at one of the departments, where patients received circa 90,000 IU of vitamin D3. We compared in-hospital mortality rates between the groups. In a subgroup analysis, we evaluated the efficacy and safety of vitamin D3 supplementation by assessing 25(OH)D and 1,25(OH)2D concentrations on days 0, 4, and 8. Results: As a result of the supplementation, the deficiency was resolved in 4 days in deficient patients, and none of the 25(OH)D or 1,25(OH)2D concentrations exceeded the normal range. Mortality was significantly lower and decreased 67% in the group receiving vitamin D3 supplementation, regardless of baseline 25(OH)D concentrations. Conclusions: The supplemental dosage, 3 × 30,000 IU of vitamin D3, is effective and safe and may reduce mortality in COVID-19 infection.