bluuebunny/crossref_metadata_2025 huggingface dataset:
Intron Retention Coupled with Nonsense-Mediated Decay is Involved in Cellulase Biosynthesis in Cellulolytic Fungi
Authors: Gao Yichen , Pang Ai-Ping , Ma Leyao , Wang Haiyan , Durrani Samran , Li Bingzhi , Wu Fu-Gen , Lin Fengming
Published: January 2022

Background

Knowledge on regulatory networks associated with cellulase biosynthesis is prerequisite for exploitation of such regulatory systems in ehancing cellulase production with low cost. The biological functions of intron retention (IR) and nonsense-mediated mRNA decay (NMD) in filamentous fungi is lack of study, let alone their roles in cellulase biosynthesis.
Result

We found that major cellulase genes (cel7a, cel7b, and cel3a) exhibited concomitant decrease in IR rates and increase in their gene expression in T. reesei under cellulase-producing condition (cellulose and lactose) that was accompanied with a more active NMD pathway, as compared to non cellulase-producing condition (glucose). In the presence of the NMD pathway inhibitor that successfully repressed the NMD pathway, the mRNA levels of cellulase genes were sharply down-regulated, but the rates of IR in these genes were significantly up-regulated. Consistently, the cellulase activities were severely inhibited. In addition, the NMD pathway inhibitor caused the downregulated mRNA levels of two important genes of the target of rapamycin (TOR) pathway, trfkbp12 and trTOR1. The absence of gene trfkbp12 made the cellulase production in T. reesei more sensitive to the NMD pathway inhibitor.
Conclusion

All these findings suggest that the IR of cellulase genes regulates their own gene expression by coupling with the NMD pathway, which might involve the TOR pathway. Our results provide better understanding on intron retention, the NMD pathway, and cellulase production mechanism in filamentous fungi.
Combinatorial Design of the MAUT and PAMSSEM II Methods for Multiple Attributes Group Decision Making with Probabilistic Linguistic Information
Authors: Kong Qiaoyu , Wu Liangping
Published: January 2022

This paper considers the application of probabilistic linguistic term sets (PLTS) in multiple-attribute group decision-making (MAGDM) when the weights can’t be determined. First, as an improvement of the PROMETHEE method, the PAMSSEM method can not only handle missing evaluations, but also proposes a rejection threshold to calculate the overall consistency of the plan, so as to rank the plan more reasonably. At the same time, the MAUT uses the marginal utility function to reallocate the attribute values of the alternatives in the interval , and then calculate the total utility to sort them. Because the utility function is beneficial in expressing consumer satisfaction, we combine the MAUT method and PAMSSEM II method and apply it to solve decision-making problems under probabilistic linguistic environment. Secondly the coefficient of variation method, entropy method and analytic hierarchy process are used to calculate the weights in a combination. In the process of data processing, we use the transfer function to convert the PLTS into the hesitant probabilistic fuzzy set (HPFS) for calculation. Finally, the PL-MAUT-PAMSSEM II method, PROMRTHE method, TOPSIS method and ARAS method are compared with each other.
Identification of Immune Infiltration, Differentially Expressed Genes, and Signaling Pathways in Fanconi Anemia Based on Bioinformatics Analysis
Authors: Shen Biyu , Shi Songsong , Chen Haoyang , Lu Yi , Cui Hengmei , Li Yunyun , Yang Dongliang
Published: January 2022

Background and Objective: Fanconi anemia (FA) patients have a reduced ability to form blood cells, accompanied by multiple congenital malformations, mental retardation, solid tumors, and other symptoms. However, the molecular mechanism that causes FA is unclear, and few studies have addressed the regulatory mechanism of immune infiltration in FA. Here, we aimed to identify differentially expressed genes (DEGs), pathways, and immune infiltration involved in FA using integrated bioinformatics analysis and molecular mechanisms. Methods: The GEO gene chip database was searched for FA low density bone marrow tissue, and the content and proportion of 22 types of immune cells in the FA group and the normal group were analyzed using CIBERSORT. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of FA differentially expressed genes (DEGs) using R language and related package programs was also performed.Results: The expression levels of T cells regulatory (Tregs), M2 macrophages, T cells CD8, dendritic cells resting, and T cells CD4 naïve in FA were higher than in the normal group. Furthermore, the expression levels of naïve B cells, monocytes, and resting mast cells in FA were lower than in the normal group. GO analysis of FA differential genes showed that “neutrophil degranulation,” “neutrophil activation,” and “neutrophil activation involved in immune response,” were most frequently enriched among biological processes, with “specific granule,” “tertiary granule,” “tertiary granule lumen” among cellular components, and “carbohydrate binding” among molecular functions. For the KEGG analysis, “Asthma” was most often enriched.Conclusion: This study obtained useful data related to immune infiltration, DEGs, and gene pathways of FA, and provides new evidence for immunotherapy and clinical assessment of FA patients. These results are potentially a useful reference for subsequent related scientific research.
Combined CTP- IGF-1 Score: A New Score for Assessment of Disease Severity in HCV Related Liver Cirrhosis
Authors: Ghannam Elsayed , Rozaik Salah , Agwa Ramy Hasan , Marwan Ahmed , Mashaly Mervat El-Sayed , Salem Mohamed Mofreh , zekred Abeer Saad El , Saleh Ahmed
Published: January 2022

Background: Liver is the largest endocrine organ in the body. It is a key organ in insulin mediated metabolism, growth hormone and insulin like growth factors (IGF) pathway. Liver cirrhosis is the end result of many chronic diseases including hepatitis C virus infection. Child-Turcotte-Pugh (CTP) score is the standard used in assessment of hepatic reserve but it has its drawbacks in the form of subjective variables, hepatic encephalopathy and ascites. The aim of this work is to assess IGF-1 in patients with liver cirrhosis, correlate it with CTP score and assess value of modified combined CTP-IGF-1 score. Patients and Methods: 170 patients with CLD (liver cirrhosis) and 72 healthy controls in the study groups were enrolled in the study. All patients were subjected to thorough history, clinical examination and laboratory assessment. IGF-1 was measured and all patients were evaluated using CTP and CTP-IGF-1 scores. Results: IGF1 showed highly significant low values in the study group in comparison to controls (42.15 ± 27.976 and 66.31 ± 33.084 ng/ml respectively, p <0.001). It showed also highly significant negative correlation to CTP score in the study group (p < 0.001) with progressive decrements with CTP score stage progression where IGF1 levels were 48.32 ± 28.611, 40.28 ± 25.869 and 18.80 ± 15.953 ng/ml (mean ± standard deviation) in relation to CTP score groups A, B and C respectively (p value < 0.001). The combined CTP-IGF-1 score in comparison to the classic CTP score showed improved area under curve (0.848 and 0.854), sensitivity (71.2% and 88%), negative predictive value (41.7% and 53.7%), false negative results (49 and 19) and accuracy (75.73% and 83.98%) but decreased specificity (97.22% and 61.1%), positive predictive value (99.2% and 91.5%) and higher false positive results (1 and 14) respectively. Conclusion: IGF-1 show progressive decrements with progression of liver cirrhosis and is negatively correlated with CTP score. Addition of IGF-1 to CTP score to formulate combined score improves the AUC, sensitivity, negative predictive value and the accuracy of CTP score and decreases the false negative results.
SARS-CoV-2, alcohol consumption, poor social behaviour, and the COVID-19 pandemic
Authors: Marques Ornelas
Published: January 2022

Studies dealing with the SARS-CoV-2 virus and the COVID-19 disease have concentrated on the study of the virus and the disease themselves, but a knowledge of the causes responsible for the pandemic is still lacking. Crucial for effective decision making and control of the pandemic is a thorough and critical analysis of data regarding COVID-19, which shows an intimate relation between the number of cases/deaths and the consumption of alcohol. Ranked top 30 countries in COVID-19 cases/deaths and alcohol consumption coincide, with only a few exceptions. This coincidence is not fortuitous nor surprising, because excessive alcohol consumption is known to have pernicious effects on social behaviour, i.e. lead to deviant and irresponsible behaviour, which greatly promotes transmission
Open or Not Open the Retroperitoneum: A Pandora’s Box for High-grade Pancreatic Trauma?
Authors: Li Kaiwei , Chen Wensong , Wang Kai , Yang Chao , Deng Yunxuan , Wang Xinyu , Duan Zehua , Chu Chengnan , Hu Yuepeng , Liu Yuxiu , Li Weiqin , Ding Weiwei
Published: January 2022

Background: The management strategy associated with the optimal clinical outcomes for patients with pancreatic trauma remains ambiguous. We sought to determine whether transitioning from initial laparotomy (LAP) to the nonoperative management strategy based on initial percutaneous drainage (PCD) without opening the retroperitoneum would improve clinical outcomes in patients with blunt high-grade pancreatic trauma.Methods: We conducted a retrospective cohort study of pancreatic trauma at a single tertiary referral center. Blunt high-grade pancreatic trauma patients with stable hemodynamics and no diffuse peritonitis were enrolled consecutively in the study. The primary outcome measure was the incidence of severe complications (Clavien-Dindo classification ≥ Ⅲb) for patients who underwent initial LAP vs PCD. To study effect modification by different initial strategies and to adjust for confounding, modified Poisson regression and sensitivity analysis based on propensity score matching and weighting were performed to estimate adjusted relative risks (aRR) and 95% confidence intervals (CIs).Results: Among 119 patients with blunt grade Ⅲ/Ⅳ pancreatic trauma (107 male [89.9%] and 12 female [10.1%]; mean age, 35.7 [SD, 12.7] years), 29 underwent initial PCD and 90 underwent initial LAP (January 2009 through October 2021). Compared with initial LAP, patients underwent initial PCD were significantly lower risk of severe complicates (9/29 [31.0%] vs 65/90 [72.2%]; aRR, 0.52 [95% CI, 0.30-0.90]). Consistent results are also observed in sensitivity analysis models. The relative risk of severe complications for the PCD group in propensity score matching model was 0.53 (95% CI, 0.28-0.99; P = 0.035), 0.37 (95% CI, 0.18-0.75; P = 0.006) in inverse probability of treatment weighting model, and 0.55 (95% CI, 0.31-0.99; P = 0.046) in overlap weighting model. In addition, the mean number of reinterventions per patient was 1.8 in the PCD group and 2.6 in the LAP group (P = 0.067). Conclusions: For blunt high-grade pancreatic trauma patients with stable hemodynamics and no diffuse peritonitis, initial PCD strategy without open the retroperitoneum has a significantly lower rate of severe complications and does not increase reinterventions compared with initial LAP. Further randomized controlled trials are warranted to validate these results.Trial Registration: ClinicalTrials.gov Identifier: NCT03681041(Sept. 21 2018).
Regulation of Life Extension Factor Klotho on Depressive-like Behaviors via Modulation of GluN2B Function in the Nucleus Accumbens
Authors: Wu Han-jun , Liu Liu-er , Wu Wen-ning , Zhan Jin-qiong , Li Yi-heng , Chen Chun-nuan , Jiang Shu-zhen , Xiong Jian-wen , Yu Zhi-Min , Wang Wei , Wei Bo , Yang Yuan-Jian
Published: January 2022

Klotho is a life extension factor that has an ability to regulate the function of GluN2B-containing N-methyl-D-aspartate receptors (NMDARs), whose dysfunction in the nucleus accumbens (NAc) underlies critical aspects of the pathophysiology of major depression. Here we study the functional relevance of klotho in the pathogenesis of depression. A chronic social defeat stress paradigm, where mice are either categorized as susceptible or unsusceptible group based on their performance in a social interaction test, was used in this study. We found that the expression of klotho was largely decreased in the NAc of susceptible mice when compared to control or unsusceptible group. Genetic knockdown of klotho in the NAc induced depressive-like behaviors in naive mice, while overexpression of klotho produced an antidepressive effect in normal mice and ameliorated the depressive-like behaviors in susceptible mice. Molecularly, knockdown of klotho in the NAc resulted in selective decreases of total and synaptic GluN2B expression that were identical to susceptible mice. Elevation of klotho in the NAc reversed the reductions of GluN2B expressions, as well as altered synaptic transmission and spine density in the NAc of susceptible mice. Furthermore, blockade of GluN2B with a specific antagonist abolished the beneficial effects of klotho elevation in susceptible mice. Collectively, we demonstrated that klotho in the NAc modulates depressive-like behaviors by regulating the function of GluN2B-containing NMDARs. These results reveal a novel role for klotho in the pathogenesis of depression, opening new insights into the molecular basis of major depression.
Postural Analysis: Description of a Dedicated System. An Upgrade
Authors: Palandri Sergio
Published: January 2022

BackgroundIn June 2021 an aericle was published describing a possible ssystem to perform postural analysis. In six months use, the ssystem has more clarely showed its lights and shadoes. So the author tought some improvements of the sustem that are now ready to be applyed. This article will show them in details.Materials and MethodsUpgrade interested both aspects close connected to the analysis and both connected to its management .ResultsFirst results obtained are interesting and encourage the use of the upgrades introduced. However a more long period of use is necessary to confirm first resultas.ConclusionsUpgrading is a natural step in every life project. And it is made up not only by a single step. So also this system may be further improved with other upgrades in the future.
Group Penalized Logistic Regressions Predict Ovarian Cancer
Authors: Xie Ying
Published: January 2022

Objectives: Ovarian cancer ranks first among gynecological cancers in terms of the mortality rate. Accurately diagnosing ovarian benign tumors and malignant tumors is of immense important. The goal of this paper is to combine group LASSO/SCAD/MCP penalized logistic regression with machine learning procedure to further improve the prediction accuracy to ovarian benign tumors and malignant tumors prediction problem. Methods: We combine group LASSO/SCAD/MCP penalty with logistic regression, and propose group LASSO/SCAD/MCP penalized logistic regression to predict the benign and malignant ovarian cancer. Firstly, we select 349 ovarian cancer patients data and divide them into two sets: one is the training set for learning, and the other is the testing set for checking, and then choose 46 explanatory variables and divide them into 11 different groups. Secondly, we apply the training set and group coordinate descent algorithm to obtain group LASSO/SCAD/MCP estimator, and apply the testing set to compute confusion matrix, accuracy, sensitivity and specificity. Finally, we compare the prediction performance for group LASSO/SCAD/MCP penalized logistic regression with that for artificial neural network (ANN) and support vector machine (SVM).Results: Group LASSO/SCAD/MCP/ penalized logistic regression selects 6/4/1 groups. The prediction accuracy and AUC for group MCP/SCAD/LASSO penalized logistic regression/SVM/ANN is 93.33%/85.71%/82.26%/74.29%/72.38% and 0.892/0.852/0.823/0.639/0.789, respectively.Conclusions: Group MCP/SCAD/LASSO penalized logistic regression performs than SVM and ANN in terms of prediction accuracy and AUC. In particular, group MCP penalized logistic regression predicts the best. Therefore, we suggest group MCP penalized logistic regression to predict ovarian tumors.
Effects of NRF-1 and PGC-1α Cooperation on HIF-1α and Rat Cardiomyocyte Apoptosis Under Hypoxia
Authors: Niu Nan , Li Hui , Du Xiancai , Wang Chan , Li Junliang , Yang Jihui , Liu Cheng , Yang Songhao , Zhu Yazhou , Zhao Wei
Published: January 2022

Hypoxia is a primary inducer of cardiomyocyte injury, its significant marker being hypoxia-induced cardiomyocyte apoptosis. Nuclear respiratory factor-1 (NRF-1) and hypoxia-inducible factor (HIF)-1α are transcriptional regulatory elements implicated in multiple biological functions, including oxidative stress response. However, their roles in hypoxia-induced cardiomyocyte apoptosis remain unknown. The effect HIF-α, together with NRF-1, exerts on cardiomyocyte apoptosis also remains unclear. We established a myocardial hypoxia model and investigated the effects of these proteins on the proliferation and apoptosis of rat cardiomyocytes (H9C2) under hypoxia. Further, we examined the association between NRF-1 and HIF-1α to improve the current understanding of NRF-1 anti-apoptotic mechanisms. The results showed that NRF-1 and HIF-1α are important anti-apoptotic molecules in H9C2 cells under hypoxia, although their regulatory mechanisms differ. NRF-1 could bind to the promoter region of Hif-1α and negatively regulate its expression. Additionally, HIF-1β exhibited competitive binding with NRF-1 and HIF-1α, demonstrating a synergism between NRF-1 and the peroxisome proliferator-activated receptor-gamma coactivator-1α. These results indicate that cardiomyocytes can regulate different molecular patterns to tolerate hypoxia, providing a novel methodological framework for studying cardiomyocyte apoptosis under hypoxia.
Autophagy Regulates the Effects of Adipose-Derived Stem Cell Small Extracellular Vesicles On Lipopolysaccharide-Induced Pulmonary Microvascular Barrier Damage
Authors: Li Chichi , Wang Min , Wang Wangjia , Li Yuping , Zhang Dan
Published: January 2022

Background: Small extracellular vesicles (sEVs) have been recognized to be more effective than direct stem cell differentiation into functional target cells in preventing tissue injury and promoting tissue repair. Our previous study demonstrated the protective effect of adipose-derived stem cells (ADSCs) on lipopolysaccharide (LPS)-induced acute lung injury and the effect of autophagy on ADSC functions, but the role of ADSC-derived sEVs (ADSC-sEVs) and autophagy-mediated regulation of ADSC-sEVs in LPS-induced pulmonary microvascular barrier damage remains unclear. Methods: After treatment with sEVs from ADSCs with or without autophagy inhibition, LPS-induced human pulmonary microvascular endothelial cell (HPMVECs) barrier damage was detected. LPS-induced acute lung injury in mice was assessed in vivo after intravenous administration of sEVs from ADSCs with or without autophagy inhibition. The effects of autophagy on the bioactive miRNA components of ADSC-sEVs were assessed after prior inhibition of cell autophagy. Results: We found that ADSC-sEV effectively alleviated LPS-induced apoptosis, tight junction damage and high permeability of PMVECs. Moreover, in vivo administration of ADSC-sEV markedly inhibited LPS-triggered lung injury. However, autophagy inhibition, markedly weakened the therapeutic effect of ADSC-sEVs on LPS-induced PMVECs barrier damage and acute lung injury. In addition, autophagy inhibition, prohibited the expression of five specific miRNAs in ADSC-sEVs -under LPS-induced inflammatory conditions. Conclusions: Our results indicate that ADSC-sEVs protect against LPS-induced pulmonary microvascular barrier damage and acute lung injury. Autophagy is a positive mediator of sEVs function, at least in part through controlling the expression of bioactive miRNAs in sEVs.
Urban Development Reduces Bee Abundance and Diversity
Authors: Pfeiffer Vera Wilder , Crowder David W. , Silbernagel Janet
Published: January 2022

Wild bee communities persist in cities despite major disruption of nesting and food resources by urban development. Bee diversity and abundance is key for urban agriculture and maintenance of plant diversity, and assessing what aspects of cities enhance bee populations will promote our capacity to retain and provision bee habitat. Here, we assessed how variation in land cover and neighborhood development history affected bee communities in the midwestern US urban landscape of Madison, Wisconsin. We sampled bee communities across 38 sites with relatively high (> 55%) or low (< 30%) levels of impervious surface, and assessed effects of land use and neighborhood development history on bee abundance and species richness. We show abundance and richness of bees was lower in recently developed neighborhoods, with particularly strong negative effects on soil nesting bees. Soil nesting bees and bee community richness decreased as cover of impervious surface increased, but above ground nesting bees were minimally impacted. Bee community similarity varied spatially and based on dissimilar local land cover, only for soil nesting bees, and the overall bee community. Impervious surface limited bee abundance and diversity, but new neighborhoods were associated with greater negative effects. We suggest that enhancing the structural diversity of new neighborhoods in urban ecosystems may imitate the structural benefits of older neighborhoods for bee populations.
An Experimental Study on the Effect of Micro-Metakaolin on the Strength and Swelling Characteristics of Expansive Soils
Authors: Sakr Mohamed , Azzam Waseim , Meguid Mohamed , Ghoneim Hebatalla
Published: January 2022

Expansive soils are found in many parts of the world, especially in arid areas and dry weather regions. Urbanization and development of new cities around the world resulted in construction in areas of challenging subsurface soil conditions. For example, in the Middle East, the Government of Egypt is building several new cities to accommodate the continuous increase in the country’s population. Most of these new cities are located in areas underlain by expansive soils. In this study, a series of laboratory tests were carried out to investigate the effect of introducing micro-metakaolin into the matrix of an expansive soil to improve the swelling potential as a new stabilizing material. Test results showed that micro-metakaolin can considerably decrease the free swell index of the soil by 37% and 54% at micro-metakaolin content of 15% and 25%, respectively. In addition, the shear strength of the soil was found to also increase as a result of the introduction of the micro-metakaolin material. Adding 25% micro-metakaolin content reduced the swelling pressure of the soil by about 33%. The results suggest that the proposed method is efficient in stabilizing and improving the properties of expansive soils found in arid areas. This is important to control excessive swelling and prevent possible damage to the supported structures.
Does Prevalence of Sexual Dysfunction Differ Among the Most Common Causes of Infertility? A Cross-Sectional Study
Authors: Ashrafi Mahnaz , Jahangiri Nadia , Sadatmahalleh Shahideh Jahanian , Mirzaei Negin , Hesari Naiiere Gharagozloo , Rostami Frahnaz , Mousavi Seyedeh Saeedeh , Zeinaloo Mona
Published: January 2022

Background

Sexuality as a fundamental component of women’s health, can be affected by infertility. The current study aimed at comparing the prevalence of sexual dysfunction among women with the most common causes of infertility.
Methods

The current cross-sectional study was conducted on 240 infertile females with infertility due to polycystic ovary syndrome (PCOS, n=80), endometriosis (n=80) and male factor (n=80) at Royan Institute for Reproductive Biomedicine (Tehran, Iran) and 160 fertile women at health care centers, between May 2016 and June 2017. Sexual function was assessed by Female Sexual Function Index (FSFI). Data were analyzed using SPSS (version 25.00) and differences were regarded statistically significant at p < 0. 05.
Results

The prevalence of female sexual dysfunction was 98.8% in women with PCOS, 100.0% in those with endometriosis, and 80.0% in those with male factor infertility. Overall, 36.2% of the enrolled fertile women were suffering from sexual dysfunction.
Conclusions

There was an association between the prevalence of female sexual dysfunction or individual domain scores of the FSFI, and infertility etiologies. Therefore, infertility care providers are required to take this into consideration and develop preventive strategies in this regard.
Factors influencing and changes in childhood vaccination coverage over time in Bangladesh: A multilevel mixed-effects analysis
Authors: Kundu Satyajit , Kundu Subarna , Seidu Abdul-Aziz , Okyere Joshua , Ghosh Susmita , Hossain Ahmed , Alshahrani Najim Z. , Banna Md. Hasan Al , Rahman Md. Ashfikur , Ahinkorah Bright Opoku
Published: January 2022

There is a dearth of information on childhood vaccination coverage in Bangladesh. Thus, this study aimed to investigate the associated factors and changes in childhood vaccination coverage over time in Bangladesh. Bangladesh's Demographic and Health Surveys from 2011, 2014, and 2017-18 provided data on vaccination coverage for children aged 12 to 35 months. For three survey periods, multilevel binary logistic regression models were employed. The overall prevalence (weighted) of full vaccination among children aged 12-35 months was 86.17% in 2011 and 85.13% in 2014, and 89.23% in 2017-18. Children from families with high wealth index, mothers with higher education, and over the age of 24 and who sought at least four ANC visits, as well as children from urban areas were more likely to receive full vaccination. Rangpur division had the highest change rate of immunization coverage from 2011 to 2014 (2.26%), whereas Sylhet division had the highest change rate from 2014 to 2017-18 (34.34%). To improve immunization coverage for Bangladeshi children, policymakers must integrate vaccine programs, paying special attention to mothers without at least a high school education and families with low wealth index. Increased antenatal care visits may also aid in increasing the immunization coverage of their children.
Study of PVDF/ (Co-ZnFe2O4 and Cu-ZnFe2O4) nanocomposite for the piezo-phototronics applications
Authors: EL-Masry Mai , Ramadan Rania
Published: January 2022

Polyvinylidene fluoride (PVDF) polymer is considered as a promising piezoelectric material whose optical properties need to be improved. Zinc ferrite is an excellent photoelectric material, in the present work it was doped separately by both cobalt and copper. Co-ZnFe2O4 and Cu-ZnFe2O4 nanoparticles were synthesized and characterized to be used as PVDF fillers, aiming to improve its optical properties. The optical properties as well as, the piezoelectric response of the prepared PVDF/ (Co-ZnFe2O4 and Cu-ZnFe2O4) nanocomposites were investigated. A remarkable improvement in the PVDF relative permittivity, optical conductivity, refractive index, non-linear susceptibility, and a great reduction in the band gap energy value is obtained by adding Co-ZnFe2O4 nanoparticles to it. However, Cu-ZnFe2O4 nanoparticles have limited improvement of the PVDF optical properties compared to the Co-ZnFe2O4 nanoparticles. The piezoelectric response of the PVDF polymer is clearly increased by the addition of both Co-ZnFe2O4 and Cu-ZnFe2O4 nanoparticles.
“Endogenous Melatonin Concentration Along with the Expression of Mitochondrial Regulator Genes is Elevated by the Serum Shock Process in the U87-MG Cell Line”
Authors: Balkhi Shayan , Jazi Marie Saghaeian , Samaei Nader Mansour , Farahmandrad Mahtab
Published: January 2022

According to the World Health Organization, glioblastoma, also known as the fourth grade in the development of astrocytoma, is a glial tumor limited to the central nervous system with a strong ability to invade the brain parenchyma. Melatonin can be generated outside of the pineal gland tissue, according to new research. Melatonin is produced by mitochondria independently but in concert with cell demands, and it plays an important function in cell cycle and metabolism regulation. As a result, we set out to investigate the association between cell metabolism and the serum shock-induced increase in endogenous melatonin, as well as the percentage of cell proliferation.Background: Melatonin can be produced in the mitochondria organelle of glioblastoma cells without the involvement of the pineal gland, according to new research. Regarding the physiological function of melatonin secreted by the pineal gland in the regulation of rhythmicity, the goal of this study was to see if the glioblastoma cell's melatonin production ability could be influenced using a typical serum shock technique established for cellular rhythm regulator.Material and methods: First, U87-MG glioblastoma cells were cultured in a DMEM medium containing 10% FBS and then cells were treated with a standard serum shock process (no FBS, 8h). The concentration of melatonin was measured using ELISA method in supernatant and cell extracts of Shock and control groups. The cell proliferation was measured by using BrdU staining and flow cytometry assessment. The gene expression levels of some mitochondria or circadian related genes including TFAM, BMAL1, PPARGC1A(PGC1-α), and DNM1L(DRP1) were measured, using qRT-PCR method.Results: In comparison to the control group, serum shock treated U87-MG glioblastoma cells had higher concentrations of cellular and released endogenous melatonin (two times). At the mRNA level, we discovered considerable upregulation of mitochondrial or circadian regulator genes (TFAM, BMAL1, PPARGC1A, and DNM1L); in the shock group compared to the control group (P <0.0002). Furthermore, although the percentage of proliferative cells (Brdu positive) was higher in the shock group, it was not statistically significant.Conclusion: The serum shock procedure has a significant impact on the U87-MG cell line's cellular activity. In terms of the study's findings, it's worth noting that an increase in endogenous melatonin concentration influences several signaling pathways within the U87-MG cell line, as seen by the increased expression of candidate genes.In light of the findings of this study, it's worth noting that further research into the role of endogenous melatonin and its effects on cancer cells is critical, and that comparing the results of normal and cancer cells can reveal the hotspots of the signaling pathways involved, which could facilitate in better understanding the biology of glioblastoma.
Accumulation of Antibiotic Resistance Genes in Pakchoi (Brassica Chinensis L.) Grown in Chicken Manure-fertilized Soil Amended With Fresh and Aged Biochars
Authors: Shi Zhiming , Zhang Peng , Liu Yan , Zhao Yonghua , Wang Congying
Published: January 2022

Biochar has been used to alleviate the contamination of antibiotic resistance genes (ARGs) in soil and to inhibit ARGs transfer from soil to plants. However, the effect of aged biochar on ARGs abundance in soil and ARGs enrichment in plants are scarcely investigated. In this study, a pot experiment was conducted to compare the effects of fresh and aged biochars on the accumulation of five typical ARGs including tetX, tetW, sul2, ermB and intI1 in a chicken manure-fertilized soil and in pakchoi (Brassica chinensis L.). Results showed that both biochars significantly decreased the abundance of tetW, sul2, and ermB and increased the abundance of tetX and intI1 in soil. However, the accumulation of all tested ARGs in pakchoi were significantly decreased by both biochars. At the lower addition rate (1%), the fresh biochar was superior to the aged biochar in decreasing the accumulation of some genes (tetW, tetX, and sul2) in pakchoi, whereas an opposite tendency was observed for other genes (ermB and intI1). As the addition rate increased to 2%, the difference between the two biochars diminished and a similar capacity of decreasing ARGs transfer was observed. The reduction in ARGs accumulation in pakchoi was highly related to the type of ARGs, the biochar addition level and the aging of biochar. Our results provide insights into the naturally aged biochar on the fate of ARGs in a soil-plant system.
Stabile SERS Encoded Silver Silica Nanocomposites for Industrial Labeling – The Case of COVID-19 Diagnosis
Authors: Xiao Can , Simón Bernat Mir , Rivera-Gil Pilar
Published: January 2022

Biosensors, especially those with a SERS readout, are required for an early and precise healthcare diagnosis. Unreproducible SERS platforms hamper clinical SERS. Here we report a synthetic procedure to obtain stabile, reproducible and robust highly-SERS performing nanocomposites for labelling. We control the NPs agglomeration and codification which results in an increased number of hot spots, thus exhibiting reproducible and superior Raman enhancement. We studied fundamental aspects affecting the plasmonic thiol bond resulting in pH exhibiting a determining role. We validated their biosensing performance by designing a SERS-based ELISA SARS-CoV-2 detections assay which exhibits limits of detection below 0.01 ng/μL.
IL35 Attenuated LPS-Induced Acute Lung Injury by Regulating Macrophage Polarization
Authors: Chen Shengsong , Xia Jingen , Zhang Yi , Zhan Qingyuan
Published: January 2022

Background Interleukin 35 (IL35) has been reported to play a role in acute lung injury (ALI); however, the current results on the relationship between IL35 and ALI are inconsistent. Therefore, we will further determine the function of IL35 in mouse ALI and its potential mechanism in this paper. Materials and Methods HE staining and Masson staining were used to evaluate lung injury in mice. Immunohistochemical staining was used to calculate the expression of IL35 p35, TLR4 and MD2 and the ratio of Bax/Bcl2 and p-P65/P65. The expression levels of IL35 EBi3, CD68, CD206 and MPO were detected by immunofluorescence staining. RT–PCR was used to examine the expression levels of IL1β and IL6. TUNEL staining was performed to detect apoptotic cells. Results Overexpression of IL35 alleviated LPS-induced acute lung injury in mice. IL35 overexpression decreased the expression of CD68 and increased the expression of CD206 in ALI mice. Furthermore, upregulation of IL35 expression obviously reduced the expression of MPO, IL1β and IL6 in lung tissues of mice with ALI. Mechanistically, IL35 suppressed the TLR4/NFκB-P65 pathway, leading to the promotion of M1 to M2 macrophage transition and inflammation relief in ALI in mice.Conclusions IL35 relieved LPS-reduced inflammation and ALI in mice by regulating M1/M2 macrophage polarization and inhibiting the activation of the TLR4/NFκB-P65 pathway.
Supply Chain Networking Models Under Fuzzy Uncertainty
Authors: Baidya Abhijit
Published: January 2022

In decision-making model, the techniques of numerical analysis have been widely adopted. It is rare for someone to solve a linear program by hand — except perhaps in a class-room. Large-scale simulations would be all but impossible without the aid of a computer. For many people, numerical techniques have superseded analytic techniques as a tool for solving mathematical problems. This paper proposed Generalized LUExponential Trapezoidal Fuzzy Number and their ranking based on numerical integration. In this ranking method, the values are calculated with left and right spreads at some 𝜶 −level of generalized LU-exponential trapezoidal fuzzy numbers and Weddle‘s rule for numerical integration. To illustrate the proposed methods, a fuzzy four dimensional transportation problem (FDTP) is proposed and solved. This ranking approach is very simple and useful for the real life inequality based decision making problems.
Effects of Overexpressed SOCS1 Transfected DCs on Th17- and Treg-Related Cytokines in Mice with COPD
Authors: Liao Shi-xia , Chen Jie , Zhang Lan-Ying , Zhang Jing , Sun Peng-Peng , Ouyang Yao
Published: January 2022

Background: In this study, we established a chronic obstructive pulmonary disease (COPD) model by stimulating mice with cigarette smoke, and observed the effects of dendritic cells (DCs) overexpressing SOCS1 on Th17, Treg and other related cytokines in peripheral blood, bronchoalveolar lavage fluid and lung tissues of COPD mice.Methods: After successfully transfecting DCs with overexpressing SOCS1 (DC-SOCS1), the mice were injected with DC-SOCS1 (1×106), DC-SOCS1 (2×106) and immature DCs (1×106) via tail vein on days 1 and 7 of COPD fumigation modeling. After day 28 of modeling, the peripheral blood, BALF and lung tissue samples were extracted from the mice, and the changes of DCs, Th17 and Treg cells and related cytokines were detected by immunohistochemistry, immunofluorescence, HE staining, flow cytometry and ELISA.Results: The results showed that DC-SOCS1 was able to reduce the secretion of pro-inflammatory factors and increase the anti-inflammatory factors in the COPD mice, and the effect of high concentration (2×106 DC-SOCS1) was better than low concentration (1×106 DC-SOCS1). Moreover, the intervention effect was significant on day 1 compared with day 7. In the mice injected with DC-SOCS1, the expression of CD83, IL-4, Foxp3, and CCR6 was increased on day 1 than those on day 7, while IL-17 and IFN-γ was decreased.Conclusions: Intervention of COPD mice with high concentrations of DCs-SOCS1 reduced pro-inflammatory factor secretion and attenuated the inflammatory response in COPD.Trial registration：Not applicable.
Ulectomia no tratamento de impactação dentária por fibrosamento gengival em paciente infantil: relato de caso
Authors: Rodrigues Amanda Borges , Danelon Marcelle , Bento Laura Imbriani , Favretto Carla Oliveira
Published: January 2022

A precoce intervenção do cirurgião dentista em casos de impacção dentária, diminui as chances de um tratamento mais complexo posteriormente, e aumentam a estética e função da cavidade oral. O trabalho tem como finalidade, apresentar a ulectomia como procedimento cirúrgico de escolha, em um caso clínico com impacção dentária por fibrose gengival. Paciente do gênero feminino, 8 anos de idade, compareceu a clínica de Odontopediatria da Faculdade Morgana Potrich – FAMP, tendo como queixa principal a ausência dos dentes da frente. Realizado o exame intra-oral constatamos que se tratava da ausência dos dentes 11 e 21 e através do exame radiográfico, foi confirmado que os dentes estavam formados 2/3 da raiz completa, sendo o suficiente para erupcionarem e ocuparem seus lugares na arcada dentária, no entanto, somente a força eruptiva não era suficiente para romper a barreira fibrótica. O plano de tratamento estabelecido foi a exérese do tecido fibroso na região incisal dos dois dentes, possibilitando passagem para se posicionarem em oclusão. Desta forma, o tratamento evitou maiores transtornos e proporcionou maior qualidade de vida a paciente, devolvendo a função e estética.
Cárie radicular entre indivíduos em situação de rua: prevalência e fatores associados
Authors: Freire Maria do Carmo Matias , Lawder Juliana Aparecida de Campos , Souza João Batista de , Matos Marcos André de
Published: January 2022

Introdução: A cárie radicular tem sido investigada na população em geral, mas a evidência em pessoas em situação de rua é limitada. Objetivo: Investigar a prevalência de cárie radicular e associação com fatores sociodemográficos, uso de drogas e saúde bucal em indivíduos adultos em situação de rua. Material e Método: Estudo transversal com 116 indivíduos de 18 a 68 anos de Goiânia, Goiás. Os dados foram coletados por meio de entrevista e exame clínico, com base nos critérios da Organização Mundial de Saúde. O desfecho foi a prevalência de cárie na raiz. As covariáveis foram as características sociodemográficas, uso de drogas lícitas e ilícitas, número de dentes remanescentes, índice CPOD e uso de prótese parcial removível. Na análise estatística foram utilizadas comparações bivariadas e regressão de Poisson com variância robusta. Resultados: A prevalência de cárie radicular foi 26,4% (IC 95%= 19,0-35,5), com média de 0,76 raízes por indivíduo. Todas as raízes afetadas estavam cariadas e não tratadas e os dentes mais afetados foram os posteriores. Maior prevalência foi encontrada em indivíduos com idade, número de dentes remanescentes e índice CPO-D mais elevados. Após ajuste, apenas a idade permaneceu associada ao desfecho (RP= 1,03; IC95%= 1,00-1,06). Conclusão: Mais de ¼ dos indivíduos em situação de rua pesquisados apresentaram cárie radicular e a prevalência foi associada ao aumento da idade. A inexistência de raízes restauradas sugere a necessidade de promover o acesso deste grupo populacional aos serviços de saúde.
Prevalência de delirium em idosos submetidos a cirurgias com anestesia geral endovenosa
Authors: Sotero Niliane Mayrink Stopa , Cerdeira Cláudio Daniel , Ferreira Sergio Luis Assis , Guimarães Roberto Salvador , Silva Roberta Bessa Veloso , Santos Gérsika Bitencourt
Published: January 2022

Introdução: anestésicos gerais (AGs) endovenosos podem gerar complicações pós-operatórias, com destaque para o delirium, uma condição que atrasa a recuperação funcional do paciente e acarreta maior custo hospitalar. Objetivo: este estudo investigou a prevalência de delirium pós-anestésico/operatório (DPAO) em um hospital Universitário. Metodologia: estudo transversal no qual foram avaliados pacientes com idade acima de 60 anos, submetidos a diferentes tipos de cirurgias utilizando AGs. O miniexame do estado mental (MEEM) foi replicado em três momentos (MMP, MM1 e MM3): durante o pré-operatório (D0), um dia após a cirurgia (D1) e três dias após a cirurgia (D3). Resultados: entre os 28 pacientes que concluíram o estudo (50% para cada sexo e faixa etária mais frequente entre 60 e 69 anos [64%]), a maior parte relatou possuir entre 1 a 4 anos de estudo (53%), e os AGs mais utilizados em ordem decrescente foram: o fentanil (85,7%), remifentamil (78,6%), propofol (64,3%) e etomidato (50%). A prevalência de DPAO foi 21,4% (n = 6), com 14,3% (n = 4) dos casos ocorrendo em D1, 3,6% (n = 1) em D3 e 3,6% (n = 1) em ambos os períodos, havendo maior prevalência em cirurgias cardíacas. Houve significativa (p < 0,05) maior pontuação média para o sexo masculino. Não foi observada diferença entre o nível de escolaridade, faixa etária, tipo de cirurgia ou uso de AGs e a pontuação no MEEM. Conclusão: a prevalência de DPAO em idosos mostrou-se significativa e apenas a variável “sexo” influenciou na média de pontuação no MEEM.
A Acupuntura é efetiva no tratamento das desordens temporomandibulares? Revisão da literatura
Authors: Reis Mateus Silva Beker dos , Ferreira Italo Augusto Silva , Oliveira Jader Bueno de , Siqueira Letícia da Costa , Andrade Loren dos Santos , Monteiro Vinicius Ribeiro , Haddad Marcela Filié
Published: January 2022

Introdução: A disfunção temporomandibular é um grupo de condições que apresentam como sintoma a dor na região da articulação temporomandibular. A etiologia dessa condição ainda é incerta, mas é sugerida como multifatorial. O foco principal no tratamento da disfunção temporomandibular é o controle da dor, e da disfunção do aparelho mastigatório de modo a atenuar cargas adversas que acabam perpetuando o problema. A acupuntura faz parte da Medicina Tradicional Chinesa, técnica que consiste basicamente na introdução de agulhas em pontos específicos da superfície corporal, e tem sido usada para promover o alívio da dor. Objetivo: avaliar a utilização da acupuntura no tratamento de desordens temporomandibulares. Materiais e métodos: Foi realizada uma revisão de literatura com busca nas bases dados PubMed, Lilacs, Medline e BBO no período de 2000 a 2020. Resultados: Foram encontrados 52 artigos, excluídos 37 artigos após a leitura de títulos e resumos, sendo incluídos 15 artigos. Conclusão: A partir da literatura pesquisada, pode-se concluir que a acupuntura é uma terapia de suporte complementar no tratamento das disfunções temporomandibulares e tem se mostrado tão eficiente no controle de dores faciais quanto as terapias convencionais.
Solução estética minimamente invasiva: uso do clareamento dental em consultório na suavização da hipomineralização molar-incisivo
Authors: Pinheiro Raíssa Freitas , Gonçalves Mariana de Oliveira , Sousa Emerson Tavares de , Madalena Isabela Ribeiro
Published: January 2022

Objetivo: Reportar o caso de um paciente com hipomineralização molar-incisivo (HMI) tratado com clareamento dental de consultório, utilizando o artificio de proteger a lesão hipomineralizada para evitar sintomatologia dolorosa e mascarar as lesões. Relato de Caso: Paciente de 22 anos, boa saúde geral, procurou atendimento com queixa principal relacionada a estética da região anterior. Ao exame clínico observou-se HMI, com severidade leve. Nos incisivos superiores e inferiores havia opacidades demarcadas branco/creme no terço incisal das superfícies vestibulares sem fratura pós-eruptiva. Nos molares havia restaurações atípicas em resina composta em estado satisfatório. O paciente não relatou sensibilidade. Para suavização das regiões hipomineralizadas, propôs-se o clareamento dental em consultório com peróxido de carbamida 35%. Como estratégia adjuvante, uma barreira gengival fotopolimerizável foi utilizada bloqueando o contato do agente clareador com as regiões hipomineralizadas. Essa estratégia objetivou prevenir sensibilidade durante a realização do clareamento e reduzir o contraste entre os defeitos de esmalte e o esmalte hígido. Não houve sensibilidade durante o procedimento. Ao final do tratamento, observou-se o dente com um croma uniforme, uma melhora perceptível na estética e na satisfação do paciente. Conclusão: O clareamento dental em consultório com bloqueio de regiões hipomineralizadas demonstrou ser uma opção adequada para suavização dos defeitos de esmaltes ocasionados pela HMI.
Situação do preenchimento das Cadernetas de Saúde da Criança em Unidades Básicas de Saúde de um município do Estado de São Paulo, Brasil
Authors: Barbosa Tais Fantinel , Ferreira Larissa Brazolotto
Published: January 2022

Introdução: A Caderneta de Saúde da Criança (CSC) é um instrumento essencial de vigilância a saúde da população infantil, pois através dela é possível obter o registro de dados sobre o crescimento e desenvolvimento, e sobre os eventos mais significativos para o desenvolvimento da criança. Além de orientações sobre cada fase de desenvolvimento e prevenções dos principais acidentes na infância, proporcionando auxílio aos pais em cada fase do desenvolvimento infantil que a criança se encontrar, instruindo-os nas principais dúvidas.  Objetivo: Verificar a condição do preenchimento das CSC e analisar se os responsáveis pelas crianças que adquirem a CSC recebem orientações sobre como utilizar a mesma, e sobre as informações contidas nela. Casuística e Métodos: Trata-se de uma pesquisa de natureza quantitativa, observacional e descritiva, com delineamento transversal. Será realizada com CSC de crianças entre 1 a 5 anos de idade, atendidas em Unidades Básicas de Saúde da cidade de Birigui-SP, Brasil. Obterá informações através de abordagem e aplicação de um questionário aos responsáveis e um formulário do tipo check list próprio para análise da CSC. Resultados: A pesquisa mostrou um baixo percentual de preenchimento dos dados de crescimento e desenvolvimento, exceto do gráfico de acompanhamento cefálico com 95% de preenchimento correto, e o Apgar 1°/ 5° minuto com 84% de preenchimentos corretos.    Conclusão: O baixo percentual de preenchimento dos dados de crescimento e desenvolvimento, demonstra a necessidade de profissionais e gestores da saúde darem maior importância a CSC, investindo na formação e capacitação dos profissionais quanto ao seu uso adequado.
Complicações em implantodontia e prótese sobre implante: revisão de literatura
Authors: Batista Diana Leite , Poluha Rodrigo Lorenzi
Published: January 2022

Desde que a utilização sistemática de implantes como possibilidade terapêutica eficaz foi comprovada, a busca por esse tratamento pelos pacientes com edentulismo foi ampliada. Ainda assim, como qualquer intervenção em um organismo vivo, o tratamento reabilitador através do uso de implantes apresenta possíveis complicações, tanto em relação aos implantes quanto as reabilitações implanto-suportadas. Por mais que o correto planejamento não impeça a ocorrência dessas falhas e complicações no tratamento implantoprotético, permitirá ao profissional uma previsibilidade desses intercorrências, possibilitando uma conduta eficiente a cada caso.