bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Foliar Spray Treatments with Various Biostimulants and Fertilisers on the Growth of M.9 Rootstock Stoolings
Authors: Świerczyński Sławomir , Bosiacki Maciej
Published: March 2022

An experiment was conducted to compare the effect of applying half a dose of a mineral fertiliser combined with a foliar treatment with four biostimulants and two fertilisers in relation to a full dose of a mineral fertiliser. The M.9 rootstock stoolings were sprayed four times during their growth. Each year of the experiment, the height, the diameter, the fresh weight of leaves and the leaf surface area of all stoolings were measured. The efficiency of rootstocks from one mother plant was assessed. Some parameters of physiological processes as well as the content of micro- and macronutrients in the leaves were also assessed. The research results showed that the reduced dose of the mineral fertiliser with foliar treatment did not significantly decrease the growth parameters of the M.9 rootstock stoolings. Some foliar treatments, especially Bioamino Plant and Bispeed, resulted in higher fresh weight and larger leaf area of the stoolings. The treatment with the Bioamino Plant biostimulant and two foliar fertilisers resulted in parameters of the physiological processes of stoolings that were the same as or better than those in the control plants. After the foliar application of two fertilisers, the content of macronutrients in the leaves of the stoolings was usually the same as in the control. The treatment with the biostimulants resulted in a lower content of most macronutrients in the plants (N, K and Ca). The only exception was the higher magnesium content than in the control after the treatment with all biostimulants and the same phosphorus content after the treatment with most of the biostimulants. The Aminoplant and Bispeed biostimulants increased the accumulation of iron in the leaves, whereas the Basfoliar 6-12-6 fertiliser resulted in higher zinc and copper content than in the control plants.
CircEZH2 Regulates Milk Fat Metabolism through miR-378b Sponge Activity
Authors: Wang Dongyang , Zhao Zhengjiang , Shi Yiru , Luo Junyi , Chen Ting , Xi Qianyun , Zhang Yongliang , Sun Jiajie
Published: March 2022

In this study, we evaluated the roles of heat-induced circEZH2 in the regulation of milk fat metabolism. CircEZH2 overexpression increased HC11 cell proliferation and decreased apoptosis. These changes were accompanied by increased expression of proliferation marker proteins (PCNA, Cyclin D, and Cyclin E) and the anti-apoptotic protein Bcl2, while expression of the pro-apoptotic proteins Bax and cleaved-caspase was reduced. SiRNA-mediated silencing of EZH2 in HC11 cells had the opposite effects. CircEZH2 overexpression promoted the uptake of a fluorescent fatty acid (Bodipy) as well as expression of the fatty acid transport-related protein CD36, lipolysis-related protein LPL, and unsaturated fatty acid metabolism-related proteins FADS1 and SCD1. Dual luciferase reporter assays verified the targeting relationship of the two ceRNA networks, circEZH2-miR378b-LPL and circEZH2-miR378b-CD36. This information provides further clarification of the role of circRNAs in milk fat regulation in addition to a theoretical basis for alleviating the effects of heat stress on milk production by dairy cows.
Study of the Fatty Acid Profile of Milk in Different Sheep Breeds: Evaluation by Multivariate Factorial Analysis
Authors: Conte Giuseppe , Palombo Valentino , Serra Andrea , Correddu Fabio , D’Andrea Mariasilvia , Macciotta Nicolò Pietro Paolo , Mele Marcello
Published: March 2022

A multivariate analysis was used to investigate the fatty acid (FA) profile in three different Italian sheep breeds: Comisana, Massese, and Sarda. A sample of 852 animals was considered: 118 Massese, 303 Comisana, 431 Sarda. Sarda sheep were divided into two groups, based on their breeding origin (298 and 133 reared in Sardinia and Tuscany, respectively). Sarda sheep, bred both in Sardinia and in Tuscany, were considered in different groups, both because in these two regions most of the sheep of this breed are reared, and because they differ in geographical characteristics and in the farming system. The individual milk FA composition of dairy ewes was analyzed with multivariate factor analysis. The extracted factors were representative of the following eight groups of fatty acids or functions: factor 1 (odd branched fatty acids and long-chain fatty acids), factor 2 (sn3_position), factor 3 (alternative biohydrogenation), factor 4 (SCD_1), factor 5 (SCD_2), factor 6 (SCD_3), factor 7 (fat secretion) and factor 8 (omega-3). A factor analysis suggested the presence of different metabolic pathways for de novo short- and medium-chain fatty acids and Δ9-desaturase products. The ANOVA of factor scores highlighted the significant effects of the breed. The results of the present study showed that breed is an important factor in defining the fatty acid profile of milk, combined with the effect of the diet. Breeds reared in the same farming system (Comisana, Massese and Sarda reared in Tuscany) showed significant differences for all the factors extracted. At the same time, we found differences between the Sarda sheep reared in Sardinia and Tuscany, two different regions of Italy.
Welfare and Performance of Post-Weaning Sows and Piglets Previously Housed in Farrowing Pens with Temporary Crating on a Spanish Commercial Farm: A Pilot Study
Authors: Ko Heng-Lun , Temple Déborah , Hales Janni , Manteca Xavier , Llonch Pol
Published: March 2022

The study investigated the effect of farrowing environment on the weaning adaptability of sows and piglets. One farrowing crate (FC) and two farrowing pens with temporary crating (TC: SWAP and JLF15) were compared. Sixty-four sows and 663 piglets were followed until 5 days post-weaning. At weaning (D24), sows and piglets were moved to group pens and nursery pens, respectively. Sows and piglets’ behaviors were observed on D24, D25, and D26. On D23, D25, and D26, piglets’ skin lesions were counted, and sows and piglets’ saliva samples were collected for stress biomarkers (cortisol and chromogranin A, CgA). Piglets were weighed on D23 and D29. All the piglets’ skin lesions increased on D25 and decreased on D26 (p < 0.05). Compared to D23, cortisol of JLF15 and CgA of FC piglets increased, whereas those of SWAP piglets remained similar after weaning (p < 0.05). Post-weaning performance in piglets was similar across farrowing systems. SWAP sows vocalized more than FC and JLF15 on D24 and D25 (p < 0.001). Results suggested that SWAP piglets showed a lower weaning stress response. Frequent post-weaning vocalization in SWAP sows might be linked with a negative effect of the abrupt separation from the piglets.
Effect of Supplementing Different Levels of L-Glutamine on Holstein Calves during Weaning
Authors: Wang Shuo , Wang Fuwei , Kong Fanlin , Cao Zhijun , Wang Wei , Yang Hongjian , Wang Yajing , Bi Yanliang , Li Shengli
Published: March 2022

Weaning stress affects the health and performance of calves. L-glutamine (L-Gln) is commonly used as a functional antioxidant and energy supplement in the body. However, dietary L-Gln supplementation improving weaning stress of calves is unclear. Thus, we aimed to explore the effects of L-Gln (provided by rumen-protected L-Gln) on calves during weaning. Seventy-five Holstein calves (54.0 ± 2.68 kg; 42 ± 2.1 d of age) were assigned to five groups: no supplementation and L-Gln with 1%, 2%, 3%, and 4% dry matter daily intake (DMI) supplementation groups, respectively. The experiment lasted for 28 days (42–70 d of age of calves), and the calves were weaned at 15 d of experiment. DMI and body weekly weight of all calves were recorded. Blood samples of nine healthy calves with similar body weight were collected from each group at 0, 7, 14, 16, 18, 21, and 28 d of experiment for detecting serum L-Gln, glucose, insulin, urea nitrogen, D-lactate, cortisol, haptoglobin, interleukin-8, immunoglobulin (Ig) G, IgA, IgM, total antioxidant capacity, superoxide dismutase, glutathione peroxidase, catalase, and malondialdehyde. At the end of the experiment, six healthy calves with similar body weight from each group were selected for slaughter and morphological analysis of small intestine tissue. The results showed that the L-Gln supplementation in the diets improved the negative effects of sudden weaning in calves. Furthermore, compared to the higher-level L-Gln supple-mentation (3 and 4% of DMI) groups, the dietary lower-level L-Gln supplementation (1 and 2% of DMI) had higher average daily gain, glutathione peroxidase and IgG concentration, and villus height/crypt depth of the duodenum and jejunum, as well as lower cortisol, haptoglobin, and interleukin-8 concentration of weaned calves. These results provided effective reference for relieving the negative effects of calves during weaning.
The Influence of Wetting Agent and Type of Nozzle on Copper Hydroxide Deposit on Sugar Beet Leaves (Beta vulgaris L.)
Authors: Sedlar Aleksandar , Gvozdenac Sonja , Pejović Momčilo , Višacki Vladimir , Turan Jan , Tanasković Snežana , Burg Patrik , Vasić Filip
Published: March 2022

Protective fungicides are sensitive to environmental conditions such as rainfall and solar radiation. Therefore, it is important to prolong the biological activity and fungicide resistance to the above-mentioned factors that can be achieved by adding a wetting agent to the working solution. Additionally, the quality and efficiency of preventive contact fungicides significantly depend on the application technique. Thus, it is important to make the right choice of the nozzles and adjust the working parameters of the treatment. The aim of this work is to determine the influence of a wetting agent and type of nozzle on copper hydroxide (2 L ha−2) deposits on sugar beet leaves. Experiments are set up under laboratory and field conditions. A pinole-based wetting agent is applied at three rates (0.3, 0.6, and 1.0 L ha−1) and two types of nozzles are used (standard with flat jet and modern turbo-drop twin-jet). A brilliant blue tracer is added to a working solution to enable the measurement of copper hydroxide deposits. The deposit amount is recorded before and after the rain simulation (15 L m−2) with a spectrophotometer light beam. In order to ensure the timeliness of the application of fungicides, remote sensing of vegetative indices is used as an indicator of disease occurrence. The results indicated an increase in copper hydroxide deposits with the increase in wetting agent rates for both types of nozzles and in both laboratory and field experiments. Moreover, when applying the copper hydroxide mixtures with modern turbo drop nozzles, the increase in copper hydroxide deposit is significant, compared to the standard nozzles.
Mechanical Static Force Negatively Regulates Vitality and Early Skeletal Development in Zebrafish Embryos
Authors: Carnovali Marta , Banfi Giuseppe , Mariotti Massimo
Published: March 2022

Skeletal system development and remodelling is regulated by several different factors, including hormones, cytokines, and mechanical forces. It is known that gravity and pressure stimulate mechanosensors on bone cells which transduce mechanical signals to chemical ones. Nevertheless, few data have been provided about the role of mechanical forces on embryo osteogenesis in vivo. Since the zebrafish is an elective model for developmental studies, in particular on bone formation and tissue mineralization, we analyzed in vivo the effects of a static mechanical force generated by a water column on fertilized zebrafish eggs. The results have shown that an increase in the hydrostatic pressure (HP) of up to 5.9% was lethal for 100% of treated embryos at 48 h post fertilization (hpf). A small decrease in length (−2%) and 49% mortality were found in the +4.4% HP embryos compared with the controls. To analyze skeletal development, we evaluated the number of mineralized vertebral bodies in the trunk at five days post fertilization. The embryos grown under +2.4% HP showed a physiological intramembranous mineralization of vertebral bodies whereas the embryos which grew with +3.4% HP showed a significant decrease in mineralization rate (−54%). Morphological analysis of cartilage and bones in embryos at +3.4% HP revealed a delay of both intramembranous and chondrogenic mineralization, respectively, in axial and head bones, whereas the chondrogenesis appeared normal. These data suggested that developing osteoblasts and different mineralization programs are sensitive to mechanical pressure when applied to early embryogenesis.
Map Construction and Path Planning Method for a Mobile Robot Based on Multi-Sensor Information Fusion
Authors: Li Aijuan , Cao Jiaping , Li Shunming , Huang Zhen , Wang Jinbo , Liu Gang
Published: March 2022

In order to solve the path planning problem of an intelligent vehicle in an unknown environment, this paper proposes a map construction and path planning method for mobile robots based on multi-sensor information fusion. Firstly, the extended Kalman filter (EKF) is used to fuse the ambient information of LiDAR and a depth camera. The pose and acceleration information of the robot is obtained through the pose sensor. The SLAM algorithm based on a fusion of LiDAR, a depth camera, and the inertial measurement unit was built. Secondly, the improved ant colony algorithm was used to carry out global path planning. Meanwhile, the dynamic window method was used to realize local planning and local obstacle avoidance. Finally, experiments were carried out on a robot platform to verify the reliability of the proposed method. The experiment results showed that the map constructed by multi-sensor information fusion was closer to the real environment, and the accuracy and robustness of SLAM were effectively improved. The turning angle of the path was smoothed using the improved ant colony algorithm, and the real-time obstacle avoidance was able to be realized using the dynamic window method. The efficiency of path planning was improved, and the automatic feedback control of the intelligent vehicle was able to be realized.
Mixed Method for Isogeometric Analysis of Coupled Flow and Deformation in Poroelastic Media
Authors: Bekele Yared Worku , Fonn Eivind , Kvamsdal Trond , Kvarving Arne Morten , Nordal Steinar
Published: March 2022

Pressure oscillations at small time steps have been known to be an issue in poroelasticity simulations. A review of proposed approaches to overcome this problem is presented. Critical time steps are specified to alleviate this in finite element analyses. We present a mixed isogeometric formulation here with a view to assessing the results at very small time steps. Numerical studies are performed on Terzaghi’s problem and consolidation of a layered porous medium with a very low permeability layer for varying polynomial degrees, continuities across knot spans and spatial discretizations. Comparisons are made with equal order simulations.
Hydrogen vs. Batteries: Comparative Safety Assessments for a High-Speed Passenger Ferry
Authors: Mylonopoulos Foivos , Boulougouris Evangelos , Trivyza Nikoletta L. , Priftis Alexandros , Cheliotis Michail , Wang Haibin , Shi Guangyu
Published: March 2022

Batteries and hydrogen constitute two of the most promising solutions for decarbonising international shipping. This paper presents the comparison between a battery and a proton-exchange membrane hydrogen fuel cell version of a high-speed catamaran ferry with a main focus on safety. The systems required for each version are properly sized and fitted according to the applicable rules, and their impact on the overall design is discussed. Hazards for both designs were identified; frequency and consequence indexes for them were input qualitatively, following Novel Technology Qualification and SOLAS Alternative Designs and Arrangements, while certain risk control options were proposed in order to reduce the risks of the most concerned accidental events. The highest ranked risks were analysed by quantitative risk assessments in PyroSim software. The gas dispersion analysis performed for the hydrogen version indicated that it is crucial for the leakage in the fuel cell room to be stopped within 1 s after being detected to prevent the formation of explosive masses under full pipe rupture of 33 mm diameter, even with 120 air changes per hour. For the battery version, the smoke/fire simulation in the battery room indicated that the firefighting system could achieve a 30% reduction in fire duration, with firedoors closed and ventilation shut, compared to the scenario without a firefighting system.
Theoretical Study of the Efficient Ion Acceleration Driven by Petawatt-Class Lasers via Stable Radiation Pressure Acceleration
Authors: Liu Meng , Gao Jia-Xiang , Wang Wei-Min , Li Yu-Tong
Published: March 2022

Laser-driven radiation pressure acceleration (RPA) is one of the most promising candidates to achieve quasi-monoenergetic ion beams. In particular, many petawatt systems are under construction or in the planning phase. Here, a stable radiation pressure acceleration (SRPA) scheme is investigated, in which a circularly-polarized (CP) laser pulse illuminates a CH2 thin foil followed by a large-scale near-critical-density (NCD) plasma. In the laser-foil interaction, a longitudinal charge-separated electric field is excited to accelerate ions together with the heating of electrons. The heating can be alleviated by the continuous replenishment of cold electrons of the NCD plasma as the laser pulse and the pre-accelerated ions enter into the NCD plasma. With the relativistically transparent propagation of the pulse in the NCD plasma, the accelerating field with large amplitude is persistent, and its propagating speed becomes relatively low, which further accelerates the pre-accelerated ions. Our particle-in-cell (PIC) simulation shows that the SRPA scheme works efficiently with the laser intensity ranging from 6.85×1021 W cm−2 to 4.38×1023 W cm−2, e.g., a well-collimated quasi-monoenergetic proton beam with peak energy ∼1.2 GeV can be generated by a 2.74 × 1022 W cm−2 pulse, and the energy conversion efficiency from the laser pulse to the proton beam is about 16%. The QED effects have slight influence on this SRPA scheme.
Parameter Estimation of the Bishop Photovoltaic Model Using a Genetic Algorithm
Authors: Restrepo-Cuestas Bonie Johana , Montano Jhon , Ramos-Paja Carlos Andrés , Trejos-Grisales Luz Adriana , Orozco-Gutierrez Martha Lucia
Published: March 2022

Photovoltaic panels can be affected by partial shading, which causes some shaded cells to consume the energy generated by other cells of the panel. That is, shaded cells stop operating in the first quadrant and start operating in the second quadrant, with negative voltage at their terminals, causing power losses and other negative effects in the cells. The Bishop model is an accurate representation of the cells behavior at the second quadrant, but estimating its parameters is not a trivial task. Therefore, this paper presents a procedure to estimate the parameters of the Bishop model by using the Chu–Beasley optimization technique. The effectiveness of this procedure was evaluated using different accuracy measures such as RMSE and MAPE, obtaining values lower than 0.5%. In addition, the results of this study demonstrate that it is essential to estimate all the parameters of the Bishop model, illustrate the variation in the parameters according to the cell technology and show the strong influence of the shunt resistance into the behavior at the second quadrant.
Health Risk from Toxic Metals in Wild Rice Grown in Copper Mining-Impacted Sediments
Authors: Sarkar Rohan Deep , Zhang Zhiming , Warke Manas , Datta Rupali
Published: March 2022

Northern wild rice is of great dietary and cultural importance to the Native American population in the Upper Peninsula of Michigan. Millions of tons of mine tailings were discharged into Lake Superior and other inland lakes during the copper mining boom in the early 20th century in this area. This includes L’Anse Bay, located within the Keweenaw Bay Indian Community (KBIC) reservation. Since wild rice restoration is being encouraged by the KBIC, we investigated the distribution of toxic metals in sediments, water, and wild rice and their potential impact on human health from two locations. Sand Point sloughs on L’Anse Bay and a nearby inland lake, Lake Plumbago, were sampled for sediment, water, and wild rice, and the potential human health risk from dietary exposure to toxic metals in wild rice was assessed. Arsenic stood out as the element that had the highest bioaccumulation at both locations. Risk calculations showed that the hazard index (HI) value for wild rice seeds from both sites was high. Data indicate both carcinogenic and non-carcinogenic risks for As from wild rice in Sand Point sloughs and Lake Plumbago, and carcinogenic risks for Cd and Cr at Lake Plumbago.
Experimental Study of 3D Concrete Printing Configurations Based on the Buildability Evaluation
Authors: Cui Hongzhi , Li Yuanhong , Cao Xiangpeng , Huang Mingyang , Tang Waiching , Li Zongjin
Published: March 2022

Different formulations for 3D printable cementitious composites have been developed for extrusion-based printing. However, there is a lack of configuration guides for actual printing operations, which integrate one printable material and one printing system closely. Three testing methods for configuration determination were proposed and tested with three material proportions, with initial setting times of 2, 8, and 13 min, respectively. The building index (BI) measures the layer stacking stability based on the material, scale, and device. The height reduction test (HRT) quantifies the shortening in the height of the printed filaments. The leaning angle (LA) refers to the maximum slope of the stacked layers. In this study, results showed the critical values were (a) 0.167 for the height reduction ratio (HRR), (b) 40° for LA, and (c) 0~19.1, 0~61.1, and 0~99.4 for BI of the three mixtures. They were the meta parameters used to guide the CAD sketching, material development, and printing configurations, including the printing speed and layer height.
Generalization of Fuzzy Connectives
Authors: Makariadis Stefanos , Papadopoulos Basil
Published: March 2022

This paper is centered around the creation of new fuzzy connectives using automorphism functions. The fuzzy connectives theory has been implemented in many problems and fields. In particular, the N-negations, t-norms, S-conorms and I-implications concepts played crucial roles in forming the theory and applications of the fuzzy sets. Thus far, there are multiple strategies for producing fuzzy connectives. The purpose of this paper is to provide a new strategy that is more flexible and fast in comparison with the rest. In order to create this method, automorphism and additive generator functions were utilized. The general formulas created with this method can provide new fuzzy connectives. The main conclusion is that new fuzzy connectives can be created faster and with more flexibility with our strategy.
Antagonistic L1 Adhesion Molecule Mimetic Compounds Inhibit Glioblastoma Cell Migration In Vitro
Authors: Nagaraj Vini , Mikhail Mirai , Baronio Micol , Gatto Alessia , Nayak Ashana , Theis Thomas , Cavallaro Ugo , Schachner Melitta
Published: March 2022

Cell adhesion molecule L1 is a cell surface glycoprotein that promotes neuronal cell migration, fosters regeneration after spinal cord injury and ameliorates the consequences of neuronal degeneration in mouse and zebrafish models. Counter-indicative features of L1 were found in tumor progression: the more L1 is expressed, the more tumor cells migrate and increase their metastatic potential. L1′s metastatic potential is further evidenced by its promotion of epithelial–mesenchymal transition, endothelial cell transcytosis and resistance to chemo- and radiotherapy. These unfortunate features are indicated by observations that cells that normally do not express L1 are induced to express it when becoming malignant. With the aim to ameliorate the devastating functions of L1 in tumors, we designed an alternative approach to counteract tumor cell migration. Libraries of small organic compounds were screened using the ELISA competition approach similar to the one that we used for identifying L1 agonistic mimetics. Whereas in the former approach, a function-triggering monoclonal antibody was used for screening libraries, we here used the function-inhibiting monoclonal antibody 324 that reduces the migration of neurons. We now show that the L1 antagonistic mimetics anagrelide, 2-hydroxy-5-fluoropyrimidine and mestranol inhibit the migration of cultured tumor cells in an L1-dependent manner, raising hopes for therapy.
Frontotemporal Lobar Degeneration Case with an N-Terminal TUBA4A Mutation Exhibits Reduced TUBA4A Levels in the Brain and TDP-43 Pathology
Authors: Van Schoor Evelien , Vandenbulcke Mathieu , Bercier Valérie , Vandenberghe Rik , van der Zee Julie , Van Broeckhoven Christine , Otto Markus , Hanseeuw Bernard , Van Damme Philip , Van Den Bosch Ludo , Thal Dietmar Rudolf
Published: March 2022

Recently, disease-associated variants of the TUBA4A gene were identified in patients with amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Here, we present the neuropathological report of a patient with the semantic variant of primary progressive aphasia with a family history of Parkinsonism, harboring a novel frameshift mutation c.187del (p.Arg64Glyfs*90) in TUBA4A. Immunohistochemistry showed abundant TAR DNA-binding protein 43 kDa (TDP-43) dystrophic neurite pathology in the frontal and temporal cortex and the dentate gyrus of the hippocampus, consistent with frontotemporal lobar degeneration (FTLD). The observed pathology pattern fitted best with that of FTLD-TDP Type C. qPCR showed the presence of mutant TUBA4A mRNA. However, no truncated TUBA4A was detected at the protein level. A decrease in total TUBA4A mRNA and protein levels suggests loss-of-function as a potential pathogenic mechanism. This report strengthens the idea that N-terminal TUBA4A mutations are associated with FTLD-TDP. These N-terminal mutations possibly exert their pathogenic effects through haploinsufficiency, contrary to C-terminal TUBA4A mutations which are thought to disturb the microtubule network via a dominant-negative mechanism.
Oxygen-Sensitive Metalloprotein Structure Determination by Cryo-Electron Microscopy
Authors: Cherrier Mickaël V. , Vernède Xavier , Fenel Daphna , Martin Lydie , Arragain Benoit , Neumann Emmanuelle , Fontecilla-Camps Juan C. , Schoehn Guy , Nicolet Yvain
Published: March 2022

Metalloproteins are involved in key cell processes such as photosynthesis, respiration, and oxygen transport. However, the presence of transition metals (notably iron as a component of [Fe-S] clusters) often makes these proteins sensitive to oxygen-induced degradation. Consequently, their study usually requires strict anaerobic conditions. Although X-ray crystallography has been the method of choice for solving macromolecular structures for many years, recently electron microscopy has also become an increasingly powerful structure-solving technique. We have used our previous experience with cryo-crystallography to develop a method to prepare cryo-EM grids in an anaerobic chamber and have applied it to solve the structures of apoferritin and the 3 [Fe4S4]-containing pyruvate ferredoxin oxidoreductase (PFOR) at 2.40 Å and 2.90 Å resolution, respectively. The maps are of similar quality to the ones obtained under air, thereby validating our method as an improvement in the structural investigation of oxygen-sensitive metalloproteins by cryo-EM.
Investigation of the Repairing Effect and Mechanism of Oral Degraded Sericin on Liver Injury in Type II Diabetic Rats
Authors: Wei Zhen-Zhen , Weng Yu-Jie , Zhang Yu-Qing
Published: March 2022

In the sericulture and silk production industry, sericin is discharged in the degumming wastewater, resulting in a large amount of wasted natural protein and environmental pollution. This study investigated the effect of degraded sericin recovered by the Ca(OH)2–ultrasound degumming method (a green process) on liver injury in T2D rats. After 4 weeks of dietary sericin supplementation, the liver masses and organ coefficients of the T2D rats improved compared with those of the model rats that were not fed sericin. Oral sericin activated the damaged PI3K/AKT/AMPK pathway to enhance glycogen synthesis, accelerate glycolysis, and inhibit gluconeogenesis. The protein expression levels of the inflammatory factors NF-κB, IL-6, and TNF-α in the T2D model group were up to two times higher than in the normal group. However, all three T2D groups that received oral sericin showed significant decreases in these factors to the level found in the normal group, indicating that inflammation in the body was significantly reduced. These results show that the sericin protein might improve glycogen synthesis, accelerate glycolysis, and inhibit gluconeogenesis by enhancing the anti-oxidation capability and reducing inflammatory reactions. Therefore, sericin recovered by Ca(OH)2 degradation has potential use in the development of functional health foods that can lower blood sugar.
Ketamine as a Novel Psychopharmacotherapy for Eating Disorders: Evidence and Future Directions
Authors: Ragnhildstveit Anya , Slayton Matthew , Jackson Laura Kate , Brendle Madeline , Ahuja Sachin , Holle Willis , Moore Claire , Sollars Kellie , Seli Paul , Robison Reid
Published: March 2022

Eating disorders (EDs) are serious, life-threatening psychiatric conditions associated with physical and psychosocial impairment, as well as high morbidity and mortality. Given the chronic refractory nature of EDs and the paucity of evidence-based treatments, there is a pressing need to identify novel approaches for this population. The noncompetitive N-methyl-D-aspartate receptor (NMDAr) antagonist, ketamine, has recently been approved for treatment-resistant depression, exerting rapid and robust antidepressant effects. It is now being investigated for several new indications, including obsessive–compulsive, post-traumatic, and substance use disorder, and shows transdiagnostic potential for EDs, particularly among clinical nonresponders. Hence, the aim of this review is to examine contemporary findings on the treatment of EDs with ketamine, whether used as a primary, adjunctive, or combination psychopharmacotherapy. Avenues for future research are also discussed. Overall, results are encouraging and point to therapeutic value; however, are limited to case series and reports on anorexia nervosa. Further empirical research is thus needed to explore ketamine efficacy across ED subgroups, establish safety profiles and optimize dosing, and develop theory-driven, targeted treatment strategies at the individual patient level.
What Do We Know about Survival in Skeletally Premature Children Aged 0 to 10 Years with Ewing Sarcoma? A Multicenter 10-Year Follow-Up Study in 60 Patients
Authors: Bosma Sarah E. , van der Heijden Lizz , Sierrasesúmaga Luis , Merks Hans J. H. M. , Haveman Lianne M. , van de Sande Michiel A. J. , San-Julián Mikel
Published: March 2022

(1) Background: Younger age has been associated with better overall survival (OS) in Ewing sarcoma (ES), especially under the age of 10. The favorable survival in younger patients underlines the need for minimizing treatment burden and late sequelae. Our study aimed at describing clinical characteristics, treatment and outcome of a cohort of ES patients aged 0–10. (2) Methods: In this retrospective multicenter study, all consecutive ES patients aged 0–10, treated in four sarcoma centers in the Netherlands (n = 33) and one in Spain (n = 27) between 1982 and 2008, with a minimum follow-up of 10 years, were included. OS, local recurrence-free survival (LRFS) and distant metastasis-free survival (DMFS) were calculated. Potential factors of influence on OS (risk and protective factors) were analyzed. (3) Results: 60 patients with median follow-up 13.03 years were included. All patients were treated with chemotherapy in combination with local treatment, being surgery alone in 30 (50%) patients, radiotherapy (RT) alone in 12 (20%) patients or surgery plus RT in 18 (30%) patients (12 pre- and 6 postoperative). Limb salvage was achieved in 93% of patients. The 10-OS, -LRFS and -DMFS are 81% (95% CI: 71–91%), 89% (95% CI: 85–93%) and 81% (95% CI: 71–91%), respectively. Six patients developed LR, of which two developed subsequent DM; all had axial ES (pelvis, spine or chest wall), and these patients all died. Ten patients developed DM; eight died due to progressive disease, and two are currently in remission, both with pulmonary metastasis only. Negative or wide resection margin was significantly associated with better OS. Age < 6 years, tumor volume < 200 mL, absence of metastatic disease and treatment after 2000 showed trends towards better OS. Two patients developed secondary malignancy; both had chemotherapy combined with definitive RT for local treatment. (4) Conclusions: Overall survival of these youngest patients with ES was very good. Limb salvage surgery was achieved in >90% of patients. Wide resection margin was the only factor significantly associated with better survival.
Understanding the Notch Signaling Pathway in Acute Myeloid Leukemia Stem Cells: From Hematopoiesis to Neoplasia
Authors: Láinez-González Daniel , Serrano-López Juana , Alonso-Dominguez Juan Manuel
Published: March 2022

The Notch signaling pathway is fundamental to early fetal development, but its role in acute myeloid leukemia is still unclear. It is important to elucidate the function that contains Notch, not only in acute myeloid leukemia, but in leukemic stem cells (LSCs). LSCs seem to be the principal cause of patient relapse. This population is in a quiescent state. Signaling pathways that govern this process must be understood to increase the chemosensitivity of this compartment. In this review, we focus on the conserved Notch signaling pathway, and its repercussions in hematopoiesis and hematological neoplasia. We found in the literature both visions regarding Notch activity in acute myeloid leukemia. On one hand, the activation of Notch leads to cell proliferation, on the other hand, the activation of Notch leads to cell cycle arrest. This dilemma requires further experiments to be answered, in order to understand the role of Notch not only in acute myeloid leukemia, but especially in LSCs.
[18F]Fluciclovine PET/CT Improves the Clinical Management of Early Recurrence Prostate Cancer Patients
Authors: Nappi Anna Giulia , Ferrari Cristina , Mammucci Paolo , Rubini Dino , Lavelli Valentina , Sardaro Angela , Pisani Antonio Rosario , Rubini Giuseppe
Published: March 2022

We investigated the [18F]Fluciclovine PET/CT reliability in the early detection of recurrent prostate cancer (PCa) and its impact on therapeutic decision making. We retrospectively analyzed 58 [18F]Fluciclovine PET/CT scans performed to identify early PCa recurrence. Detection rate (DR) and semiquantitative analysis were evaluated in relation to biochemical and clinical–histological features. Clinical follow-up data were collected and considered as gold standard to evaluate sensitivity, specificity, accuracy, positive and negative predictive value (PPV, NPV). The impact of [18F]Fluciclovine PET/CT on clinical management was also assessed. Overall DR resulted as 66%, while DR was 53%, 28%, and 7% in prostate/bed, lymph nodes, and bone, respectively. DR significantly increased with higher PSA values (p = 0.009) and 0.45 ng/mL was identified as the optimal cut-off value. Moreover, SUVmax and SUVmean resulted significant parameters in interpreting malignant from benign findings. [18F]Fluciclovine PET/CT reached a sensitivity, specificity, PPV, NPV, and accuracy of 87.10%, 80.00%, 87.10%, 80.00%, and 84.31%, respectively. Therapeutic strategy was changed in 51% of patients. Our results support [18F]Fluciclovine PET/CT as a reliable tool for early restaging of PCa patients, especially for local recurrence detection, leading to a significant impact on clinical management. Semiquantitative analysis could improve specificity in interpreting malignant from benign lesions.
Towards a Molecular Classification of Sinonasal Carcinomas: Clinical Implications and Opportunities
Authors: Taverna Cecilia , Agaimy Abbas , Franchi Alessandro
Published: March 2022

Sinonasal carcinomas are a heterogeneous group of rare tumors, often with high-grade and/or undifferentiated morphology and aggressive clinical course. In recent years, with increasing molecular testing, unique sinonasal tumor subsets have been identified based on specific genetic alterations, including protein expression, chromosomal translocations, specific gene mutations, or infection by oncogenic viruses. These include, among others, the identification of a subset of sinonasal carcinomas associated with HPV infection, the identification of a subset of squamous cell carcinomas with EGFR alterations, and of rare variants with chromosomal translocations (DEK::AFF2, ETV6::NTRK and others). The group of sinonasal adenocarcinomas remains very heterogeneous at the molecular level, but some recurrent and potentially targetable genetic alterations have been identified. Finally, poorly differentiated and undifferentiated sinonasal carcinomas have undergone a significant refinement of their subtyping, with the identification of several new novel molecular subgroups, such as NUT carcinoma, IDH mutated sinonasal undifferentiated carcinoma and SWI/SNF deficient sinonasal malignancies. Thus, molecular profiling is progressively integrated in the histopathologic classification of sinonasal carcinomas, and it is likely to influence the management of these tumors in the near future. In this review, we summarize the recent developments in the molecular characterization of sinonasal carcinomas and we discuss how these findings are likely to contribute to the classification of this group of rare tumors, with a focus on the potential new opportunities for treatment.
The Impact of Treatment for Smoking on Breast Cancer Patients’ Survival
Authors: Singareeka Raghavendra Akshara , Kypriotakis George , Karam-Hage Maher , Kim Seokhun , Jizzini Mazen , Seoudy Kareem S. , Robinson Jason D. , Barcenas Carlos H. , Cinciripini Paul M. , Tripathy Debu , Ibrahim Nuhad K.
Published: March 2022

Background: Smoking negatively affects overall survival after successful breast cancer (BC) treatment. We hypothesized that smoking cessation would improve survival outcomes of BC patients who were smokers at the time of diagnosis. Methods: This was a retrospective analysis of self-identified smokers with BC treated at The University of Texas MD Anderson Cancer Center. Patient demographics, date of diagnosis, tumor stage, tobacco treatment program (TP) participation, and time to death were extracted from our departmental databases and institutional electronic health records. We examined associations between tobacco abstinence status and survival using survival models, with and without interactions, adjusted for personal characteristics and biomarkers of disease. Results: Among all 31,069 BC patients treated at MD Anderson between 2006 and 2017, we identified 2126 smokers (6.8%). From those 2126 self-identified smokers, 665 participated in the TP, reporting a conservative estimate of 31% abstinence (intent-to-treat) 9 months into the program. Patients without reported follow-up abstinence status (including TP and non-TP participants) were handled in the analyses as smokers. Survival analysis controlled for multiple factors, including disease characteristics and participation in the TP, indicated that abstainers were more likely to be alive with no evidence of disease compared to non-abstainers (HR, 0.593; 95% CI, 0.386–0.911; p = 0.017). Conclusion: Our results suggest that quitting smoking is associated with improved survival among BC patients who were smokers at time of diagnosis across all tumor stages. Comprehensive approaches for smoking cessation in patients diagnosed with BC may prolong survival when started as early as the time of diagnosis.
Mice with Targeted Knockout of Tetraspanin 3 Exhibit Reduced Trabecular Bone Mass Caused by Decreased Osteoblast Functions
Authors: Xing Weirong , Pourteymoor Sheila , Kesavan Chandrasekhar , Gomez Gustavo A. , Mohan Subburaman
Published: March 2022

Tetraspanin3 (TSPAN3) was identified as a binding partner of claudin11 (CLDN11) in osteoblasts and other cell types. Mice with targeted disruption of Cldn11 exhibited trabecular bone mass deficit caused by reduced bone formation and osteoblast function. To determine if the disruption of CLDN11 interacting protein gene Tspan3 results in a similar skeletal phenotype as that of Cldn11 knockout (KO) mice, we generated homozygous Tspan3 KO and heterozygous control mice and characterized their skeletal phenotypes at 13 weeks of age. Micro-CT measurements of the secondary spongiosa of the distal femur revealed 17% and 29% reduction in trabecular bone volume adjusted for tissue volume (BV/TV) in the male and female mice, respectively. Similarly, trabecular BV/TV of the proximal tibia was reduced by 19% and 20% in the male and female mice, respectively. The reduced trabecular bone mass was caused primarily by reduced trabecular thickness and number, and increased trabecular spacing. Consistent with the reduced bone formation as confirmed by histomorphometry analyses, serum alkaline phosphatase was reduced by 11% in the KO mice as compared with controls. Our findings indicate that TSPAN3 is an important positive regulator of osteoblast function and trabecular bone mass, and the interaction of TSPAN3 with CLDN11 could contribute in part to the bone forming effects of Cldn11 in mice.
Pathogenic Exploitation of Lymphatic Vessels
Authors: Magold Alexandra I. , Swartz Melody A.
Published: March 2022

Lymphatic vessels provide a critical line of communication between peripheral tissues and their draining lymph nodes, which is necessary for robust immune responses against infectious agents. At the same time, lymphatics help shape the nature and kinetics of immune responses to ensure resolution, limit tissue damage, and prevent autoimmune responses. A variety of pathogens have developed strategies to exploit these functions, from multicellular organisms like nematodes to bacteria, viruses, and prions. While lymphatic vessels serve as transport routes for the dissemination of many pathogens, their hypoxic and immune-suppressive environments can provide survival niches for others. Lymphatics can be exploited as perineural niches, for inter-organ distribution among highly motile carrier cells, as effective replicative niches, and as alternative routes in response to therapy. Recent studies have broadened our understanding of lymphatic involvement in pathogenic spread to include a wider range of pathogens, as well as new mechanisms of exploitation, which we summarize here.
T Cells, Interleukin-2 and Systemic Lupus Erythematosus—From Pathophysiology to Therapy
Authors: Mak Anselm
Published: March 2022

The phenotypic and functional complexities of T cells engender complicated and often confusing concepts as to how T cells ignite, accelerate and brake the inflammatory processes involved in systemic lupus erythematosus (SLE), let alone the plasticity of T cells that takes place under different immunological contexts. Nevertheless, being one of the prime survival factors of T cells, interleukin (IL)-2 plays a potentially critical role in many immunological scenarios during the pathophysiological process of SLE. Here, the pathophysiology of lupus T cells and current, as well as ongoing, therapeutic approaches of SLE that involve low-dose IL-2 administration will be highlighted. The mechanisms of IL-2 deficiency in SLE pathophysiology, the effects of low-dose IL-2 on T cells and restoration of lupus manifestations in murine SLE models, as well as the efficacy and safety of clinical trials that evaluated low-dose IL-2-containing regimens in patients with SLE will be discussed.
Biomechanical Characteristics of the Typically Developing Toddler Gait: A Narrative Review
Authors: Liu Wei , Mei Qichang , Yu Peimin , Gao Zixiang , Hu Qiuli , Fekete Gustav , István Bíró , Gu Yaodong
Published: March 2022

Independent ambulation is one of the most important motor skills in typically developing toddlers. Gait analysis is a key evaluation method in basic and clinical research. A narrative review on the literature of toddler gait development was conducted following inclusion criteria, explicitly including the factors of English article, age range, no external intervention during the experimental process of studies involved, the non-symptomatic toddler, and no pathological gait. Studies about toddlers’ morphological, physiological, and biomechanical aspects at this developmental stage were identified. Remarkable gait characteristics and specific development rules of toddlers at different ages were reported. Changes in gait biomechanics are age and walking experience-dependent. Gait patterns are related to the maturation of the neuro and musculoskeletal systems. This review thus provides critical and theoretical information and the nature of toddler walking development for clinicians and other scientific researchers. Future studies may systematically recruit subjects with more explicit criteria with larger samples for longitudinal studies. A particular design could be conducted to analyze empirically before practical application. Additionally, the influence of external interventions on the development of toddler gait may need consideration for gait development in the toddler cohort.
Study of Radiation Resistance to Helium Swelling of Li2ZrO3/LiO and Li2ZrO3 Ceramics
Authors: Abyshev Baurzhan , Shlimas Dmitriy I. , Zdorovets Maxim V. , Arshamov Yalkunzhan K. , Kozlovskiy Artem L.
Published: March 2022

The key aim of this paper is to study the presence effect of LiO impurity phases in Li2ZrO3 ceramics on the resistance to helium swelling and structural degradation during implanted helium accumulation in the near-surface layer structure. The hypothesis put forward is based on a number of scientific papers, in which it was reported that the presence of two or more phases in lithium-containing ceramics led to a decrease in the rate of radiation damage and gas swelling due to the presence of additional interfacial boundaries that prevent the point defect accumulation. As a result of the evaluation of the crystal structure deformation, it was found that the presence of the LiO impurity phase in the structure of Li2ZrO3 ceramics led to a threefold decrease in the deformation of the crystal lattice due to helium swelling at doses of 5 × 1017–5 × 1018 ion/cm2. At the same time, the nature of the crystal lattice deformation for different ceramic types is different: in the case of Li2ZrO3 ceramics, an anisotropic distortion of the crystal structure is observed, in the case of Li2ZrO3/LiO ceramics, the crystal lattice deformation has an isotropic nature.