bluuebunny/crossref_metadata_2025 huggingface dataset:
Anterior Cervical Discectomy and Fusion Using Zero-P System for Treatment of Cervical Spondylosis: A Meta-Analysis
Authors: Guo Zhaoyang , Wu Xiaolin , Yang Shuai , Liu Chang , Zhu Youfu , Shen Nana , Guo Zhu , Su Weiliang , Wang Yan , Chen Bohua , Xiang Hongfei
Published: December 2021

Objective. The current study aimed to explore the efficacy of Zero profile intervertebral fusion system (Zero-P) and traditional anterior plate cage system (PC) in the treatment of cervical spondylotic myelopathy (CSM). Further, the present study evaluated effects of the treatments on medical security, height of intervertebral disc, adjacent-level ossification development (ALOD), and adjacent segmentation disease (ASD) through a systematic retrospective analysis. Methods. Studies on Zero-P system and traditional anterior plate cage system for ACDF in the treatment of CSM were searched in PubMed, Web of Science, Ovid, Embase, and Cochrane Library databases. Two independent researchers screened articles, extracted data, and evaluated the quality of the articles based on the inclusion and exclusion criteria of the current study. RevMan5.3 software was used for meta-analysis following the guidelines of Cochrane collaboration network. Cervical curvature, interbody fusion rate, preoperative and postoperative disc height index (DHI), fusion cage sinking rate, postoperative dysphagia, ASD, ALOD, and loosening of screw were compared between the two groups. Results. A total of 17 literatures were included in the present study, including 6 randomized controlled trials and 11 observational studies. The studies comprised a total of 1204 patients with CSM, including 605 patients in the Zero-P system group (Zero-P group) and 599 patients in the traditional animal plate cage group (PC group). Results of this meta-analysis showed that postoperative dysphagia [OR = 0.40, CI (0.28, 95% 0.58),

P

 < 0.00001], ALOD [OR = 0.09, CI (0.02, 95% 0.39),

P

 = 0.001], ASD [OR = 0.42, CI (0.20, 95% 0.86),

P

 = 0.02], and screw loosening [OR = 0.20, CI (0.08, 95% 0.52),

P

 = 0.0009] of the Zero-P group were significantly lower compared with the PC group. On the other hand, preoperative cervical curvature [WMD = −0.23, CI (−1.38, 95% 0.92),

P

 = 0.69], postoperative cervical curvature [WMD = −0.38, CI (−1.77, 95% 1.01),

P

 = 0.59], cage sinking rate [OR = 1.41, CI [0.52, 95% 3.82],

P

 = 0.50], intervertebral fusion rate [OR = 0.76, CI (0.27, 95% 2.48),

P

 = 0.38], preoperative DHI [WMD = −0.04, CI (−0.14, 95% 0.22),

P

 = 0.65], and postoperative DHI [WMD = 0.06, CI (−0.22, 95% 0.34),

P

 = 0.675] were not significantly different between the two groups. Conclusion. It was evident that the Zero-P system used in ACDF is superior compared with the traditional anterior plate cage system in postoperative dysphagia, avoiding ALOD, ASD, and screw loosening.
Study of Homogeneous Reservoir Pressure Inversion Model Based on Permeability Mechanics and Interpretation Software Design
Authors: Chen Zhongshuai , Ni Hongjian , Sun Zhiqi , Zhang Shiping , Wang Qisong
Published: December 2021

Well test analysis is required during the extraction of oil and gas wells. The information on formation parameters can be inverted by measuring the change in wellbore pressure at production start-up or after well shutdown. In order to calculate the characteristic parameters of the well, this paper creates a well test interpretation model for homogeneous reservoirs based on the theory of seepage mechanics, uses the Stehfest–Laplace inversion numerical inversion algorithm, and builds the Gringarten–Bourdet logarithmic curves model. The model can be used to evaluate the homogeneous reservoir. We use this model to design the pressure inversion interpretation software to implement a pressure inversion method based on permeability mechanics theory by using computer. The software can obtain the reservoir characteristic parameters such as permeability (

K

), skin coefficient (

S

), and wellbore storage coefficient (

C

). The homogeneous formation Gringarten–Bourdet curves data are available at https://github.com/JXLiaoHIT/Study-of-homogeneous-reservoir-pressure-inversion-model.
Platelet-Rich Fibrin Used in Regenerative Endodontics and Dentistry: Current Uses, Limitations, and Future Recommendations for Application
Authors: Arshad Sohaib , Tehreem Fatima , Rehab khan Muhammad , Ahmed Fatima , Marya Anand , Karobari Mohmed Isaqali
Published: December 2021

Regenerative endodontics has introduced numerous procedures such as pulp implantation, revascularization, and postnatal stem cell therapy. Revascularization has been successfully implemented clinically nowadays, thus providing dentists with outrageous results. Platelet-rich fibrin (PRF) used either alone or along with bone graft promotes bone growth and vascularization. This matrix promotes migration, cell attachment, and proliferation of osteoblast that leads to bone formation. PRF consists of a packed fibrin complex consisting of leukocytes, cytokines, and glycoproteins such as thrombospondin. The usage of PRF has reported high success rates in surgical cases such as sinus lift procedures, healing of extraction sockets, and management of periapical abscesses. Compared to platelet-rich plasma, PRF is more economical, easy to prepare, and feasible to use in daily clinical practices. Revascularization compromised the induction of a blood clot into the root canal space, which emerged as a clinical triumph. This further led to platelet concentrates as an autologous scaffold on which revascularization could occur. The applications of PRF in regenerative endodontics are numerous, such as an agent for repairing iatrogenic perforation of the pulpal floor and for the revascularization of immature permanent teeth with necrotic pulps. It acts as a matrix for tissue ingrowth. Evidence of progressive thickening of dentinal walls, root lengthening, regression in the periapical lesion, and apical closure was reported. Further studies are needed to clarify the precise mechanism of action of PRF for dental pulp regeneration both in vitro and in vivo. The current review aims at the present uses of PRF in regenerative endodontics dentistry and its application with future recommendations and limitations.
miR-381-3p Involves in Glioma Progression by Suppressing Tumor-Promoter Factor ANTXR1
Authors: Dong Zhiqiang , Zhang Jinglong , Niu Liang , Hou Guokuo , Gao Zhenshan , Yang Qiang
Published: December 2021

Accumulating studies revealed association between development of glioma and miRNA dysregulation. A case in point is miR-381-3p, but its mechanism in glioma is unclear yet. In this work, we confirmed that overexpressed miR-381-3p repressed biological functions of glioma cells. Additionally, we also discovered that upregulated anthrax toxin receptor 1 (ANTXR1) was negatively mediated by miR-381-3p. We further proved that miR-381-3p-targeted ANTXR1 was able to counteract the suppression of miR-381-3p on biological functions of glioma. We concluded that miR-381-3p and ANTXR1 were both important factors in modulating glioma progression. miR-381-3p/ANTXR1 axis is expected to be a molecular target for glioma.
The Pressure Buildup Well Test Analysis considering Stress Sensitivity Effect for Deepwater Composite Gas Reservoir with High Temperature and Pressure
Authors: Gao Yihua , Jiang Ruizhong , Xu Xiangdong , Sun Zhaobo , Yuan Zhiwang , Ma Kang , Jiang Bin , Kang Botao , Chen Guoning , Li Chenxi
Published: December 2021

Some deepwater gas reservoirs with high temperature and pressure have obvious stress sensitivity effect resulting in difficulty in well test interpretations. The influence of stress sensitivity effect on the pressure drawdown well test is discussed in many papers. However, the influence on the pressure buildup well test is barely discussed. For practices in oilfields, the quality of pressure data from the drawdown stage of well test is poor due to the influence of production fluctuation. Thus, the pressure data from the buildup stage is used for well test interpretations in most cases. In order to analyze the influence of stress sensitivity effect on the pressure buildup well test, this paper establishes a composite gas reservoir pressure buildup well test model considering the stress sensitivity effect and the hysteresis effect. Numerical solutions to both pressure drawdown and buildup well test models are obtained by the numerical differentiation method. The numerical solutions are verified by comparing with analytical solutions and the homogeneous gas reservoir well test solution. Then, the differences between pressure drawdown and buildup well test curves considering the stress sensitivity effect are compared. The parameter sensitivity analysis is conducted. Compared with the conventional well test curve, the pressure derivative curve of pressure drawdown well test considering the stress sensitivity effect deviates upward from the 0.5 horizontal line at the inner zone radial flow stage, while it deviates upward from the M/2 (mobility ratio/2) horizontal line at the outer zone radial flow stage. However, for the pressure buildup well test curve considering the stress sensitivity effect, the pressure derivative curve gradually descends to the 0.5 horizontal line at the inner zone radial flow stage, while it descends to the M/2 (mobility ratio/2) horizontal line at the outer zone radial flow stage. The pressure derivative curve of pressure buildup well test considering the hysteresis effect is higher than the curve without considering the hysteresis effect, because the permeability cannot be recovered to its original value in the buildup stage after considering the hysteresis effect. Meanwhile, skin factor and mobility ratio have different effects on pressure drawdown and buildup well test curves. Based on the model, a well test interpretation case from a deepwater gas reservoir with high temperature and pressure is studied. The result indicates that the accuracy of the interpretation is improved after considering the stress sensitivity effect, and the skin factor will be exaggerated without considering the stress sensitivity effect.
Performance Analysis of Gas Wells Based on the Conventional Decline Parameters and the Flow Integral Equation
Authors: Zhang Lixia , Li Yong , Song Xinmin , Wang Mingxian , Yu Yang , He Yingxu , Zhao Zeqi
Published: December 2021

The estimation of reserves and performance prediction are two vital tasks for the development of gas reservoirs where the evaluation of gas in place or well-controlled reserves, as the foundation of the performance analysis of gas wells, turns to be exceedingly significant. Advanced production data analysis or modern rate transient analysis (RTA) methods mainly depend on the iterative calculations of material balance quasitime (tca) and type curve fitting, the essence of which is to update the average reservoir pressure data time and again. The traditional Arps’ decline models are of empirical nature despite the convenience and applicability to the constant bottomhole pressure (BHP) condition. In order to avoid the implicit iteration, this paper develops an explicit method for estimating the average reservoir pressure on the basis of dynamic material balance equation (DMBE), termed “flow integral method,” which can be applied to various gas production systems under boundary-dominated flow (BDF). Based on the flow integral method and the decline parameter evaluation, we employ the hyperbolic decline model to model the gas well performance at a constant BHP. The analytical formulations of decline rate and decline exponent are deduced from the DMBE and the static material balance equation (SMBE) considering the elastic compressibilities of rock pore and bound water. The resulting decline parameter method for explicit estimation of gas reserves boasts a solid and rigorous theory foundation that production rate, decline rate, and average reservoir pressure profiles have reference to each other, and its implementation steps are explained in the paper. The SMBE can, combined with the estimated pressure profile by the flow integral method, also be used to determine gas reserves which is not limited to the constant-BHP condition and can calibrate the estimates of the decline parameter method. The proposed methods are proven effective and reliable with several numerical cases at different BHPs and a field example.
Construction and Analysis of Intelligent English Teaching Model Assisted by Personalized Virtual Corpus by Big Data Analysis
Authors: Zhu Jinxia , Zhu Changgui , Tsai Sang-Bing
Published: December 2021

At present, a new round of scientific and technological revolution and industrial transformation with information technology at its core are accelerating. At present, a new round of scientific and technological revolution and industrial transformation with information technology at its core is accelerating. The challenge of new economy and new industry has put forward new requirements for the training of talents in China. The challenges of new economy and new industry have put forward new requirements for the cultivation of engineering talents in China. Based on corpus, this study constructed a model of intelligent English teaching assisted by virtual corpus. The traditional teaching of college English reading is based on, around, and for texts. Using DDL model, teachers can break the limitation of textbooks. On the basis of analyzing the general idea of the text, they can search out massive real corpus related to the general idea of the text by searching the core words in the text, so as to provide extensive reading resources for students in the maximum range. At the same time, teachers can rely on the corpus to design different types of teaching activities, realize student-centered task-based, inquiry-based, and autonomous learning and cultivate students’ critical thinking ability, practical ability, and cross-cultural communication ability. This model breaks the limitation of “classroom + textbook,” realizes student-centered task-based, exploratory, and autonomous learning, trains interdisciplinary new engineering talents needed by emerging industries and new economy in the future, and promotes the sustainable development of English teaching. Corpus-data-driven college English teaching mode breaks the limitation of “classroom + textbook,” changes the traditional college English teaching mode, and realizes student-centered task-oriented, inquiry-based, and autonomous learning.
Investigation of the Mechanism of Zishen Yutai Pills on Polycystic Ovary Syndrome: A Network Pharmacology and Molecular Docking Approach
Authors: Chen Yingyin , Chai Xinyi , Zhao Ying , Yang Xinqian , Zhong Caiting , Feng Yihui
Published: December 2021

Background. Zishen Yutai Pills (ZSYTP) is a prescription based on traditional Chinese medicine used to treat kidney-deficient pattern in traditional Chinese medicine. It is also widely used clinically for the treatment of polycystic ovary syndrome (PCOS) with positive results. This study aims to explore the potential pharmacological mechanism of ZSYTP for the treatment of PCOS by a network pharmacology approach. Methods. Compounds were collected from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform and Bioinformatics Analysis Tool for Molecular mechanism of Traditional Chinese Medicine and TCM Database@ Taiwan, and the corresponding targets were retrieved from PubChem, Swiss Target Prediction, STITCH, and DrugBank. Meanwhile, PCOS targets were retrieved from the GeneCards database, the Online Mendelian Inheritance in Man database, National Center for Biotechnology Information Database, and DrugBank. Subsequently, multiple network construction and gene enrichment analyses were conducted with Cytoscape 3.8.2 software. Based on the previous results in the study, molecular docking simulations were done. Results. 205 active compounds and 478 ZSYTP target genes were obtained after screening by ADME consideration. 1881 disease-related targets were obtained after removing duplicates. 148 intersection target genes between drug and disease targets were isolated. Gene ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes analysis highlighted multiple gene functions and different signaling pathways to treat PCOS. Further molecular docking demonstrated the practicality of in vivo action of ZSYTP to a certain extent. Conclusions. It is possible that the pharmacological effect of ZSYTP on PCOS is linked to the hypoxia-inducible factor 1 (HIF-1) signaling pathway, improving insulin resistance, the variation on gene expression such as RNA splicing, and regulation of mRNA metabolic process. This study paves the way for further research investigating its mechanisms.
Fault-Tolerant Partition Resolvability of Cyclic Networks
Authors: Azhar Kamran , Zafar Sohail , Kashif Agha , Ojiema Michael Onyango
Published: December 2021

Graph invariants provide an amazing tool to analyze the abstract structures of networks. The interaction and interconnection between devices, sensors, and service providers have opened the door for an eruption of mobile over the web applications. Structure of web sites containing number of pages can be represented using graph, where web pages are considered to be the vertices, and an edge is a link between two pages. Figuring resolving partition of the graph is an intriguing inquest in graph theory as it has many applications such as sensor design, compound classification in chemistry, robotic navigation, and Internet network. The partition dimension is a graph parameter akin to the concept of metric dimension, and fault-tolerant partition dimension is an advancement in the line of research of partition dimension of the graph. In this paper, we compute fault-tolerant partition dimension of alternate triangular cycle, mirror graph, and tortoise graphs.
Experimental Study of the Creep Disturbance Effect and Acoustic Emission Characteristics of Mudstone with Different Moisture Contents
Authors: Yu Yongjiang , Wang Pengbo , Zhang Shipeng , Liu Jingjing
Published: December 2021

It is important to have a clear understanding of the creep characteristics of water‐rich soft rocks under a dynamic load and the evolution of cracks because soft rock roadways in deep mines are very sensitive to disturbances, and instability and damage can easily occur under the impact of disturbances such as mining and blasting. In this study, a self‐developed disturbed creep test bench was used to conduct graded loading creep disturbance tests on mudstone specimens with different moisture contents. The results show that an increase in the moisture content leads to a significant increase in the creep failure strain of mudstone, and the accelerated creep rate is greatly accelerated. Moreover, as the moisture content increases, the type of mudstone creep disturbance failure gradually changes from accelerated creep failure to disturbance failure. By analyzing the acoustic emission (AE) characteristics of the mudstone creep disturbance tests, it was found that the increase in the moisture content greatly weakens the AE count and the accumulated energy. In each stage of disturbance, the AE signals jumped, and the stability was restored at the end of the disturbance. As the load increased, the specimen entered the accelerated creep stage, the AE signal increased exponentially, and the internal cracks expanded rapidly until failure occurred. It is of great significance to carry out creep disturbance experiments and to analyze the evolution of the internal cracks in specimens with different moisture contents to maintain the long‐term stability of deep soft rocks.
Icariin and Icariside II Reciprocally Stimulate Osteogenesis and Inhibit Adipogenesis of Multipotential Stromal Cells through ERK Signaling
Authors: Zhang Dawei , Zhao Ning , Wan Chao , Du Jikun , Lin Jiantao , Wang Hongbo
Published: December 2021

Herba Epimedii is a famous Chinese herbal medicine for treating bone diseases. Icariin and icariside II, the main chemical constituents, have attracted great attention from scientists for their potential as antiosteoporosis agents. Our study aimed to evaluate their effects on the lineage commitment of multipotential stromal cells (MSCs). The osteogenesis and adipogenesis of MSCs were assessed by ALP activity, calcium deposition, and adipocyte formation. The expression profiles and levels of osteogenic and adipogenic specific genes were evaluated by cDNA microarray and quantitative real-time PCR. The involvement of extracellular signal-regulated kinase (ERK) signaling was studied by enzyme-linked immunosorbent assay. Icariin and icariside II significantly increased ALP activity and mineralization during osteogenic differentiation of MSCs. Runx2, Col1, and Bmp2 were upregulated in the presence of icariin and icariside II. Meanwhile, they downregulated Pparg, Adipsin, and Cebpb expression during adipogenic differentiation. cDNA microarray revealed 57 differentially expressed genes during lineage commitment of MSCs. In addition, icariin and icariside II enhanced the phosphorylation of ERK, and the above biological effects were blocked by ERK inhibitor U0126. Icariin and icariside II may drive the final lineage commitment of MSCs towards osteogenesis and inhibit adipogenesis through the ERK signaling pathway. Both of them exert multiple osteoprotective effects and deserve more attention for their medicinal and healthcare prospects.
Logical Intelligent Detection Algorithm of Chinese Language Articles Based on Text Mining
Authors: Zheng Zihui
Published: December 2021

With the advent of the big data era and the rapid development of the Internet industry, the information processing technology of text mining has become an indispensable role in natural language processing. In our daily life, many things cannot be separated from natural language processing technology, such as machine translation, intelligent response, and semantic search. At the same time, with the development of artificial intelligence, text mining technology has gradually developed into a research hotspot. There are many ways to realize text mining. This paper mainly describes the realization of web text mining and the realization of text structure algorithm based on HTML through a variety of methods to compare the specific clustering time of web text mining. Through this comparison, we can also get which web mining is the most efficient. The use of WebKB datasets for many times in experimental comparison also reflects that Web text mining for the Chinese language logic intelligent detection algorithm provides a basis.
A Varactor-Tunable Rasorber Using Tripole and Ring Slot Elements
Authors: Guo Qingxin , Chen Qian , Su Jianxun , Li Zengrui
Published: December 2021

In this study, a frequency-selective rasorber with a tunable passband and two absorptive bands is presented. It is designed using two active FSSs, an absorptive FSS realized with tripole elements, and a lossless bandpass FSS achieved with ring slots. Both active FSSs embedded with varactors realize the shift of transmission frequency bands by controlling the bias voltage of the feed network. The working principle is briefly investigated according to an equivalent circuit model. A prototype is fabricated and measured to verify the simulated results, which show that a passband is tuned from 3 to 4.78 GHz between two absorptive bands, and the maximum band of |S11| < −10 dB covers from 2.2 to 7.96 GHz.
An Improved Structural Analysis Method for Isolator with Quasi‐Zero‐Stiffness Characteristic
Authors: Zhang Wei , Li Xiaoping , Li Jian , Li Xiqiu
Published: December 2021

A typical quasi‐zero‐stiffness (QZS) vibration isolator consisting of a vertical spring and two oblique springs has been widely researched on its static and dynamic characteristics. A general criterion for determining structural parameters of QZS isolator is to achieve low nondimensional stiffness around the equilibrium position. However, lower nondimensional stiffness of linear isolator means lower isolation frequency, which may be invalid on QZS isolator. Because there is an implicit relationship between geometric parameter and stiffness ratio of QZS isolator, this study presents an improved optimization criterion for determining the optimal structural parameters of the typical QZS isolator. The optimization criterion is that the QZS isolator has the maximum displacement range around the equilibrium position without exceeding given natural frequency, rather than given nondimensional stiffness. The results show that isolator with these optimal parameters can achieve lower stiffness around the equilibrium position and better vibration isolation performance. Furthermore, an extended QZS isolator consisting of vertical spring with fixed stiffness and prestressed oblique springs is discussed to further improve stiffness characteristic. Better stiffness performance can be obtained when the prestressed oblique springs have softening stiffness and the exponent of the nonlinear stiffness is 2. Considering the existence of friction in practical application, the influence of friction on both static and dynamic characteristics is investigated. The analysis reveals that friction has little influence on its stiffness characteristic around the static equilibrium position and friction damping produced by friction affects the response amplitude and resonant frequency in dynamics.
Activation of YAP1 by N6-Methyladenosine–Modified circCPSF6 Drives Malignancy in Hepatocellular Carcinoma
Authors: Chen Yunhao , Ling Zhenan , Cai Xianlei , Xu Yongfang , Lv Zhen , Man Da , Ge Jiangzhen , Yu Chengkuan , Zhang Deguo , Zhang Yanpeng , Xie Haiyang , Zhou Lin , Wu Jian , Zheng Shusen
Published: December 2021

Circular RNAs (circRNA) and N6-methyladenosine (m6A) modification are extensively involved in the progression of diverse tumors, including hepatocellular carcinoma (HCC). However, the cross-talk between circRNAs and m6A remains elusive in the pathogenesis of HCC. Here we investigated m6A-mediated regulation of circRNAs in HCC. m6A-related circRNAs were identified by integrating information from two published studies, revealing circular cleavage and polyadenylation specific factor 6 (circCPSF6) as a novel m6A-modified circRNA. circCPSF6 was dominated by ALKBH5-mediated demethylation, followed by the recognization and destabilization by YTHDF2. Meanwhile, circCPSF6 was upregulated in HCC specimens, and elevated circCPSF6 expression served as an independent prognostic factor for worse survival of patients with HCC. Loss-of-function assays demonstrated that circCPSF6 maintained cell proliferation and tumorigenicity and reinforced cell motility and tumor metastasis. circCPSF6 triggered expression of YAP1, further activating its downstream cascade. Mechanistically, circCPSF6 competitively bound PCBP2, blunting its binding to YAP1 mRNA, thereby sustaining the stability of YAP1. Functionally, removal of YAP1 reversed the effects of circCPSF6 in vitro and in vivo. Aberrant activation of the circCPSF6-YAP1 axis promoted HCC malignancy. These findings offer novel insights into the regulation of circRNAs by m6A modifications and the role of this epigenetic reprogramming in HCC.

Significance:

This study advances the understanding of the interplay between m6A methylation and circRNAs in hepatocellular carcinoma, highlighting the potential of circCPSF6 as a therapeutic target.
A Simple and Rapid Light-Initiated Chemiluminescence Assay for Quantitation of Artemisia-Specific Immunoglobulin E
Authors: Liu Dandan , Zhang Bei , Zhu Lina , Zheng Lisheng , Li Shaoshen , Tan Xin , Yu Yang , Li Huiqiang
Published: December 2021

Background: Light-initiated chemiluminescence assay (LICA) is a homogeneous assay that has been successfully used for the quantitation of food allergen-specific immunoglobulin E (sIgE), but not inhaled allergen-sIgE. Simultaneously, current assays used to detect allergen-sIgE are serum consuming and/or time consuming. Hence, we established a method for the quantitation of Artemisia-sIgE based on LICA and verified its performance according to the clinical guideline documents, laying a foundation for the quantitation of inhaled and food allergen-sIgE in parallel on LICA. Methods: The assay was established after optimizing the first incubation time and the dilutions of Artemisia-coated chemibeads, biotinylated goat anti-human IgE, and serum. In order to quantitate Artemisia-sIgE, the calibration curve was established with a high positive serum of known concentration. The assay performance was confirmed per the clinical guideline documents. In addition, the correlation between the results of LICA and capture enzyme-linked immunosorbent assay was evaluated. Results: The developed LICA’s coefficients of variation of repeatability and intermediate precision were 3.20%, 2.14%, and 3.85% and 4.30%, 4.00%, and 4.40%, respectively. The limit of detection was 0.10 kUA/L, and the limit of quantitation was 0.11 kUA/L. The range of linearity was from 0.27 kUA/L to 97.53 kUA/L (r = 0.9968). The correlation coefficient (r) for the correlation analysis between results of LICA and capture ELISA was 0.9087. This assay was successfully applied in 64 human serum samples, showing good sensitivity (82.20%) and specificity (100%). Conclusion: An Artemisia-sIgE quantitation assay based on LICA was successfully established. Its performance satisfied the clinical requirements and could be widely used in clinical laboratories.
CircRNAPTK2 Promotes Cardiomyocyte Apoptosis in Septic Mice by Competitively Binding to miR-29b-3p with BAK1
Authors: Xiao Hui , Fu Quanzhu , Min Li
Published: December 2021

Objective: Sepsis is a predominant reason for the growing morbidity and mortality in the world. The role of circular RNAs (CircRNAs) is actively researched in sepsis. In this study, we attempt to find out the effect of CircRNA protein tyrosine kinase 2 (circPTK2) on cardiomyocyte apoptosis in septic mice. Methods: Septic mouse model was established by cecal ligation and puncture. Then circPTK2 expression was detected and the role of circPTK2 in myocardial damage was assessed after circPTK2 expression was silenced using Ad-sh-circHIPK3. The subcellular localization of circPTK2 was analyzed. Besides, the binding relation between circPTK2 and microRNA (miR)-29b-3p and between miR-29b-3p and BCL2 antagonist/killer 1 (BAK1) was verified. The expression of miR-29b-3p and BAK1 in the myocardium was detected. Functional rescue was conducted to evaluate the role of miR-29b-3p and BAK1 in cardiomyocyte apoptosis in septic mice. Results: CircPTK2 was highly expressed in the myocardium of septic mice, while circPTK2 silencing relieved the cardiac function and reduced inflammatory reaction and cardiomyocyte apoptosis of septic mice. Mechanically, circPTK2 competitively bound to miR-29b-3p to upregulate BAK1 mRNA level. Inhibition of miR-29b-3p and BAK1 overexpression could counteract the protective role of circPTK2 silencing in the myocardium of septic mice. Conclusion: CircPTK2 is overexpressed in the myocardium of septic mice. CircPTK2 competitively bound to miR-29b-3p to upregulate BAK1 mRNA level, to promote cardiomyocyte apoptosis, inflammatory response, and myocardial damage of the myocardium of septic mice.
Bilateral Paragangliomas in the Setting of Autonomic Dysfunction: A Case Report
Authors: Shen Jerry , Ryck Angela , Chan Iris , McFadden Kaitlin S. , Hohler Anna D.
Published: December 2021

In 2018, a 59-year-old female patient presented with hoarseness in her voice, headache, intermittent pain in her right side, difficulty of right arm movement, left side neck pain, difficulty controlling hypertension of unknown etiology, and a large mass on the upper left side of her neck with a smaller mass on the right side. MRI of the neck revealed masses at each carotid bifurcation. These were determined to be bilateral paragangliomas. Paragangliomas are rare tumors, and bilateral ones tremendously so. The patient underwent radiation over 2 years, resulting in the successful shrinking and stabilization of both masses. Since completing radiation, the patient reported improvement in her memory, and her blood pressure has stabilized with medication.
An Analysis of 20 Cases of Radiation-Associated Sarcoma, Including 4 Cases Treated by Carbon Ion Radiotherapy
Authors: Negishi Takahito , Matsunobu Akira , Endo Makoto , Yokoyama Ryouhei , Kusano Shuhei , Furubayashi Nobuki , Taguchi Kenichi , Shioyama Yoshiyuki , Iida Keiichiro , Fujiwara Toshifumi , Setsu Nokitaka , Matsumoto Yoshihiro , Nakashima Yasuharu , Kohashi Kenichi , Yamamoto Hidetaka , Oda Yoshinao , Nakamura Motonobu
Published: December 2021

Introduction: Radiation-associated sarcoma (RAS) is one of the most life-threatening complications associated with the treatment of malignant neoplasms. Because all RAS patients have a history of radiotherapy, there have been no effective treatment options when RAS is not completely resected. Methods: We retrospectively reviewed 20 RAS patients, including 4 unresectable cases treated by carbon ion radiotherapy (CIRT). Results: The primary diseases targeted by radiotherapy included malignant lymphoma (n = 4), cervical cancer (n = 3), pharyngeal cancer (n = 3), breast cancer (n = 2), lung cancer (n = 1), rectal cancer (n = 1), maxillary cancer (n = 1), synovial sarcoma (n = 1), and benign neoplasms (n = 4). The histological diagnoses of RAS included osteosarcoma (n = 8), leiomyosarcoma (n = 3), undifferentiated pleomorphic sarcoma (n = 3), rhabdomyosarcoma (n = 1), angiosarcoma (n = 1), malignant peripheral nerve sheath tumor (n = 1), spindle cell sarcoma NOS (n = 1), and sarcoma not further specified (n = 2). The median survival time from the diagnosis of RAS was 26 months. Eleven patients underwent surgery. Five of these patients achieved a continuous disease free (CDF) status or showed no evidence disease. Four patients underwent CIRT. One of these patients with leiomyosarcoma achieved a CDF status, and the other patient with osteosarcoma achieved a partial response. On the other hand, 2 patients experienced grade 3 toxicities that required surgical treatment. Conclusion: RAS originates from various types of diseases that are treated by radiotherapy and shows diverse pathological features. Complete resection achieves a good prognosis. CIRT can be an effective and feasible option for unresectable RAS.
Pesquisas em Filologia e Língua Portuguesa: Vol. 1
Authors: Gil Beatriz Daruj , Cardoso Elis de Almeida , Módolo Marcelo , Campos Maria Inês Batista , Fachin Phablo Roberto Marchis
Published: December 2021

Os capítulos deste volume consistem em resultados de pesquisas desenvolvidas por pós-graduandos do Programa de Filologia e Língua Portuguesa da Faculdade de Filosofia, Letras e Ciências Humanas da Universidade de São Paulo (FFLCH-USP). Inserem-se em três linhas do Programa, a saber: Léxico do Português, Gramáticas do Português e de Línguas de Contato, e Linguística Aplicada ao Português. Nessas três linhas, desenvolvem-se, respectivamente, as pesquisas que têm por base o estudo do léxico em seus aspectos etimológicos, morfológicos, semânticos e discursivos; o estudo da gramática – fonologia, morfologia, sintaxe – tanto do português quanto de línguas em contato; o estudo do texto e do discurso aplicado tanto ao contexto escolar quanto a outros contextos. A despeito de serem trabalhos conduzidos em perspectivas variadas, reúnem-se em torno do estudo da língua portuguesa, objeto ao qual inúmeros pesquisadores vêm se dedicando ao longo dos 50 anos de existência do Programa. Para dar maior divulgação a esse conjunto de estudos, o Programa promove, desde 2018, o Colóquio de Pesquisa Discente (CoPeD), evento bienal no qual estudantes compartilham suas pesquisas, são avaliados por pesquisadores externos ao programa e têm a oportunidade de publicar seus trabalhos em obras como esta. Os 18 capítulos deste volume são resultado dos trabalhos apresentados no II CoPeD, realizado em dezembro de 2020, e abordam a neologia, tanto na língua geral, quanto no discurso literário; a terminologia; a etimologia; a ideologia e a expressividade das escolhas lexicais e seus efeitos de sentido; a importância histórica dos topônimos; as características do português africano; as variações linguísticas; as expressões que se tornam comuns na língua; aspectos textuais e discursivos em obras didáticas.
Abstract 10276: Impact of Time-to-Compression on Out-of-Hospital Cardiac Arrest Survival Outcomes: A National Registry Study
Authors: Goh Jia Ling , Pek Pin Pin , Fook-Chong Stephanie , Ho Andrew , Leong Benjamin S , Mao Desmond , Ng Wei Ming , TIAH LING , Chia Michael , Tham Lai Peng , Shahidah Nur , Arulanandam Shalini , Ong Marcus E
Published: December 2021

Introduction:
Time To first Compression (TTC) in out-of-hospital cardiac arrests (OHCA) is thought to be an important predictor of survival outcomes. Guidelines such as the Journal of American College of Cardiology Scientific Expert Panel recommend that extracorporeal membrane oxygenation (ECMO) should not be used on OHCA patients with TTC > 10 minutes. However, there is no literature validating this exclusion criterion on OHCA survival and neurological outcomes. This study aimed to evaluate the difference in neurological outcomes and survival to discharge of patients with CPR administered after 10 minutes, compared to within 10 minutes.

Methodology:
Data of OHCAs from 2012-2017 in Singapore were extracted from our national OHCA registry. We compared patients who received CPR within 10 minutes versus those who received CPR after 10 minutes. Primary outcomes were favorable cerebral performance (Glasgow-Pittsburgh Cerebral Performance Categories 1 and 2), and secondary were survival to hospital discharge or 30-day-survival.

Results:
There were 12,771 OHCAs analyzed, with 5,704 patients with TTC </=10 minutes and 7,067 with TTC >10 minutes. Fewer patients survived to hospital discharge/30
th
day with TTC > 10 minutes (aOR 0.43, 95%CI: 0.32-0.58) and survival with good neurological outcomes was lower (aOR 0.51; 95%CI: 0.41-0.62). Other significant predictors of good neurological outcomes included age (aOR 0.98, 95%CI: 0.98 - 0.99), witnessed arrest (aOR 2.39, 95%CI: 1.69-3.40), bystander AED (aOR 1.55, 95%CI: 1.12-2.26), and presence of shockable rhythm (aOR 8.76, 95%CI: 7.12-10.78). A cut-off of 17.5 minutes (aOR 0.43, 95%CI:0.28-0.65) resulted in <1% (127 out of 12,771) chance of survival with good neurological function based on the medical definition of Futility (specificity 0.84 sensitivity 0.84 ROC 0.91).

Conclusion:
Our study showed that there is a significant difference in survival and favourable neurological outcomes when TTC was >10 minutes. However, more data-driven cut-off timings/criteria should be considered instead of the arbitrary 10 minutes for eligibility of ECMO therapy.
Abstract 10336: ECPR
2
: Expert Consensus on PeRcutaneous Cannulation for Extracorporeal CardioPulmonary Resuscitation
Authors: Schmitzberger Florian F , Haas Nathan L , Coute Ryan A , Bartos Jason , Hackmann Amy E , Haft Jonathan , Hsu Cindy H , Hutin Alice , Lamhaut Lionel , Marinaro Jonathan , Nagao Ken , Nakashima Takahiro , Neumar Robert W , Shinar Zack M , Whitmore Sage , Yannopoulos Demetris ,   ECPR2 research group, Peterson William
Published: December 2021

Introduction:
Extracorporeal cardiopulmonary resuscitation (ECPR) has emerged as a promising resuscitation strategy for select patients suffering out-of-hospital cardiac arrest (OHCA), though limited data exists regarding detailed best practices for the complex process of initiating ECPR following OHCA.

Hypothesis:
Expert consensus using a modified Delphi process can systematically identify detailed best practices for ECPR initiation following adult non-traumatic OHCA.

Methods:
We utilized a modified Delphi process consisting of two survey rounds and a virtual consensus meeting to systematically identify best practices for ECPR initiation following adult non-traumatic OHCA. A modified Delphi process builds content validity and is an accepted method to develop consensus by eliciting expert opinions through multiple rounds of questionnaires. Consensus was achieved when items reached a high level of agreement, defined as greater than 80% responses for a particular item rated a 4 or 5 on a 5-point Likert scale.

Results:
Snowball sampling generated a panel of 14 content experts, composed of physicians from four continents and four primary specialties. Seven existing institutional protocols for ECPR cannulation following OHCA were identified and merged into a single comprehensive list of 216 items. The panel ultimately reached consensus on 95 items: Prior to Patient Arrival (8 items), Inclusion Criteria (8), Exclusion Criteria (7), Patient Arrival (8), ECPR Cannulation (21), Go On Pump (17), and Post-Cannulation (26).

Conclusion:
We present a list of essential items for initiation of ECPR following adult non-traumatic OHCA, generated using a modified Delphi process from an international panel of content experts. These findings can benefit centers currently performing ECPR for OHCA in quality assurance and performance improvement, and can serve as a template for new ECPR programs to follow.
Abstract 10368: The Safety and Tolerability of Prolonged Inhalation of Hydrogen Gas in Air: A Phase I Clinical Trial
Authors: Cole Alexis , Sperotto Francesca , Carlisle-Larsen Stephanie , Rivkin Michael J , Dinardo James A , Kheir John N
Published: December 2021

Introduction:
Ischemia reperfusion injury (IRI) is central to many critical illnesses, including cardiac arrest, myocardial infarction, and stroke. Inhaled hydrogen gas (H2) has been shown to diminish IRI in a number of preclinical models, in part by the selective reduction of oxyradical species.

Methods:
Following FDA and IRB approval of this Investigator-Initiated Phase I IND study (NCT04046211), 8 healthy participants underwent hospitalized exposure to 2.4% H2 in medical air via high flow nasal cannula (15 LPM) for either 24 hours (n=2), 48 hours (n=2), or 72 hours (n=4). Screening for adverse effects included periodic nurse-adjudicated assessments using Common Terminology Criteria for Adverse Events (CTCAE, v5.0); regularly-spaced vital signs, pulmonary function testing, and mini-mental state exam (MMSE); EKG and serologic testing prior to versus following exposure. Data were compared over time using Wilcoxon matched-pairs signed rank test. All AE assignments were verified by two clinicians external to the study team and an external DSMB.

Results:
Eight adult participants (18-30 years; 50% female; 62% non-Caucasian) completed the study without early termination. No symptoms or clinically significant adverse events occurred in any patient. Compared with baseline measures, there were no significant changes over time in blood pressure, heart rate, oxygen saturation, or respiratory rate (A). There were no significant changes in PFTs (B), MMSE scores (C), EKG measurements (D) or serologic tests for hematologic (except for clinically-insignificant increases in hematocrit and platelet counts), renal, hepatic, pancreatic, or cardiac injury (E) associated with H2 inhalation.

Conclusion:
Inhalation of 2.4% H2 gas does not appear to cause clinically significant adverse effects in healthy adults. These data will be foundational to future interventional studies of inhaled H2 in injury states, including following cardiac arrest.
Abstract 10522: Early Lactate Clearance is Associated With Survival in a National Cohort of Refractory Out-of-Hospital Cardiac Arrest Patients Managed With Extracorporeal Cardiopulmonary Resuscitation
Authors: Gregers Emilie , Linde Louise , Moerk Sivagowry , Andreasen Jo B , smerup morten , Kjaergaard Jesper , Moeller-Soerensen Peter Hasse , holmvang lene , TERKELSEN CHRISTIAN juhl J , Tang Mariann , christensen steffen , Moller Jacob E , Lassen Jens F , Winther-Jensen Matilde , Thomassen Sisse , Laugesen Helle , Hassager Christian , Soeholm Helle
Published: December 2021

Introduction:
In refractory out-of-hospital cardiac arrest (OHCA), prolonged whole-body ischemia with global tissue injury proceeds even after achievement of reperfusion with extracorporeal cardiopulmonary resuscitation (eCPR).

Hypothesis:
Biomarkers reflecting ischemia and inflammation may be used for prognostication of refractory OHCA managed with eCPR.

Methods:
This nationwide retrospective study included patients (≥18 years) with refractory OHCA managed with eCPR in Denmark (2011 to 2020). Biomarker levels at admission, at 24 hours (lactate, leukocytes, and platelets), and repeated measures of lactate after eCPR initiation were analyzed. Lactate clearance was calculated as (admission lactate - post eCPR lactate) / admission lactate x100%.

Results:
Two hundred eighteen patients (80% male; age 52±12yrs) were included. Primary cause of OHCA was acute myocardial infarction (63%), 69% had shockable primary rhythm and 86% witnessed OHCA with a median low-flow time of 105 minutes (Q1-Q3: 86-124 min.). Fifty-three (24%) survived to hospital discharge. Survivors had a significantly lower lactate level at admission (13.1 vs. 15.4 mmol/l, p=0.004) and after 24 hours (2.8 vs. 5.0, p=0.001), and a significantly higher admission platelet level (181 vs. 153 x10
9
/l, p=0.03). No difference in admission levels of leukocytes nor leukocytes and platelets after 24 hours were found between survivors and non-survivors. Lactate clearance was available for 68% and 79% of patients alive after 8 and 24 hours, respectively. All survivors had a lactate level less than admission lactate at 8 hours (lactate clearance >0%) and had cleared >25% of admission lactate at 24 hours after eCPR initiation. There was a significant difference in survival between quartiles of lactate clearance at 8 hours after eCPR initiation (Figure 1).

Conclusion:
In conclusion, early lactate clearance after eCPR initiation was associated with survival to discharge in refractory OHCA-patients.
Abstract 10611: Variation in Out-of-Hospital Cardiac Arrest Survival Across EMS Agencies
Authors: Garcia R , McNally Bryan , Girotra Saket , Chan Paul S ,   The CARES Surveillance Group
Published: December 2021

Background:
Although some studies have reported variation in out-of-hospital cardiac arrest (OHCA) survival by neighborhood and geographic region, little is known about variation in OHCA survival at the level of EMS agencies—which, unlike neighborhoods and regions, may have modifiable resuscitation practices.

Methods:
Within the national Cardiac Arrest Registry to Enhance Survival, we identified 258,320 non-traumatic OHCAs from 764 EMS agencies with ≥10 OHCAs annually between 2015-2019. Using multivariable hierarchical logistic regression, we computed risk-adjusted rates of survival to hospital admission for each EMS agency. We quantified the extent of variation in survival with the median odds ratios (MOR) and assessed the extent to which variation in survival was explained by two EMS agency resuscitation practices: time from 911 call to EMS arrival and the proportion of OHCAs at each EMS agency with termination of resuscitation (TOR) without meeting TOR futility criteria.

Results:
Of 258,320 persons with OHCA, mean age was 62.2 ± 17.0 years and 36.1% were female. Overall, 85.0% were of presumed cardiac etiology, 82.3% occurred at home, 44.0% were witnessed by a bystander, and ~75% were due to a non-shockable initial rhythm. Across the 764 EMS agencies, the median risk-adjusted rate of survival to hospital admission was 27.4% (IQR, 24.5% - 30.2%). The adjusted MOR was 1.35 (95% CI: 1.32, 1.39), suggesting that the odds of survival to hospital admission after an OHCA varied by 35% in two identical patients in one randomly selected EMS agency vs. another. EMS agencies in the lowest quartile of risk-adjusted survival had a mean EMS response time of 12.0 ± 3.4 minutes, whereas those in the highest quartile had a mean EMS response time of 9.0 ± 2.6 minutes (
P
<0.001). The mean proportion of OHCA cases where CPR was terminated in the field without meeting TOR futility criteria was 27.9% ±16.1% in quartile 1 and 18.9% ±11.4% in quartile 4 (
P
<0.001). Adjustment for the EMS-level variation in both resuscitation practices attenuated the MOR to 1.30 (95% CI: 1.27, 1.33).

Conclusions:
Rates of survival to hospital admission for OHCA vary significantly by EMS agency, and some of this variation in survival is explained by differences in EMS arrival time and TOR practice patterns.