bluuebunny/crossref_metadata_2025 huggingface dataset:
Systematic Analysis of Composition, Interfacial Performance and Effects of Pulmonary Surfactant Preparations on Cellular Uptake and Cytotoxicity of Aerosolized Nanomaterials
Authors: Huck Benedikt , Hidalgo Alberto , Waldow Franziska , Schwudke Dominik , Gaede Karoline I. , Feldmann Claus , Carius Patrick , Autilio Chiara , Pérez-Gil Jesus , Schwarzkopf Konrad , Murgia Xabier , Loretz Brigitta , Lehr Claus-Michael
Published: October 2021

The interplay of particles with pulmonary surfactant, a lipid‐protein material pivotal for lung function, is hypothesized as a key factor that has not been routinely considered in the current in vitro models when determining the fate of inhaled nanomaterials. To explain its influence on cellular uptake and protective effects, nanoparticles are studied on two models of alveolar cells, in the absence or presence of pulmonary surfactant. Composition and interfacial performance of native human and porcine surfactants, a commercially available bovine surfactant (Alveofact), and an artificial lung lining fluid are characterized using shotgun lipidomics and biophysical approaches (i.e., Langmuir surface balances and captive bubble surfactometry). Plain and aminofunctionalized silica nanoparticles and a novel antimycobacterial nanoformulated benzothiazinone (BTZ043) are selected as examples of neutral, positively charged and therapeutically relevant nanoparticles, respectively. They are deposited onto monocultures of human alveolar epithelial and phagocytic cell lines in the presence or absence of the surfactant preparations, modeling the alveolar milieu. Only surfactant preparations with high interfacial activity and distinctive composition mitigated the toxicity of aerosolized particles, along with a tendency of aerosolized particles to aggregate. Key requirements of surfactant preparations needed when studying interactions of nanomaterials with the pulmonary air‐blood barrier in vitro are identified.
Application of the anatomical fiducials framework to a clinical dataset of patients with Parkinson’s disease
Authors: Abbass Mohamad , Gilmore Greydon , Taha Alaa , Chevalier Ryan , Jach Magdalena , Peters Terry M. , Khan Ali R. , Lau Jonathan C.
Published: October 2021

Establishing spatial correspondence between subject and template images is necessary in neuroimaging research and clinical applications such as brain mapping and stereotactic neurosurgery. Our anatomical fiducial (AFID) framework has recently been validated to serve as a quantitative measure of image registration based on salient anatomical features. In this study, we sought to apply the AFIDs protocol to the clinic, focusing on structural magnetic resonance images obtained from patients with Parkinson’s disease (PD). We confirmed AFIDs could be placed to millimetric accuracy in the PD dataset with results comparable to those in normal control subjects. We evaluated subject-to-template registration using this framework by aligning the clinical scans to standard template space using a robust open preprocessing workflow. We found that registration errors measured using AFIDs were higher than previously reported, suggesting the need for optimization of image processing pipelines for clinical grade datasets. Finally, we examined the utility of using point-to-point distances between AFIDs as a morphometric biomarker of PD, finding evidence of reduced distances between AFIDs that circumscribe regions known to be affected in PD including the substantia nigra. Overall, we provide evidence that AFIDs can be successfully applied in a clinical setting and utilized to provide localized and quantitative measures of registration error. AFIDs provide clinicians and researchers with a common, open framework for quality control and validation of spatial correspondence and the location of anatomical structures, facilitating aggregation of imaging datasets and comparisons between various neurological conditions.
Investigation of the First Sorption Cycle of White Portland Cement by 1H NMR
Authors: Nagmutdinova Anastasiia , Brizi Leonardo , Fantazzini Paola , Bortolotti Villiam
Published: October 2021

This work is focused on the effects of drying/re-saturation cycles on the structure and components of White Portland Cement (WPC) samples. In particular, WPC of 0.5 water-to-cement ratio was studied by 1H Nuclear Magnetic Resonance (NMR) Relaxometry. A number of NMR sequences and different data processing methods were applied. Short (14 days of re-saturation) and long (6 months for the whole cycle) sorption cycles were investigated, using two drying methods: oven drying and drying under controlled relative humidity. The evolution during drying/re-saturation processes of interlayer space, gel pores, and bigger pores was followed by both the transverse relaxation times and the intensities of the nuclear magnetization. As a result of drying, reversible and irreversible changes in the pore structure, especially concerning the smaller porosity (interlayer and gel pores), were seen. Likewise, 1H signals in the crystalline phases of the cement were investigated by the standard Quadrature Echo method and by a more informative analysis based and a proper acquisition and processing of the longitudinal relaxation data. This analysis allowed the signal separation of 1H nuclei with higher and lower mobility (“solid” components). The NMR signal from this last component was analyzed on the basis of the Pake-Doublet theory in the time domain and two components were clearly detected, and assigned to 1H nuclei of crystalline water in Ettringite and OH groups in Portlandite. Reversible changes of the solid components of the cement samples were observed. This is a new method to deeply investigate the changes of solid components during sorption cycles.
Combining acoustic telemetry with a mechanistic model to investigate characteristics unique to successful Atlantic salmon smolt migrants through a standing body of water
Authors: Lilly Jessie , Honkanen Hannele M. , McCallum Joseph M. , Newton Matthew , Bailey David M. , Adams Colin E.
Published: October 2021

The Atlantic salmon, Salmo salar Linnaeus 1758, is a charismatic, anadromous species that has faced dramatic declines throughout its range. There is currently a lack of information on the effect of free-standing bodies of water on a key life event, sea migration, for the species. This study extends our understanding in this area by combining acoustic telemetry with a correlated random walk model to try to examine potential morphological and behavioural factors that differentiate successful from unsuccessful migrants through Scotland’s largest lake. Consistent with other studies, we found that smolts experienced a high rate of mortality in the lake (~ 43%), with approximately 14% potentially predated upon by birds and 4% by Northern pike. Migration speed in the lake was slow (the mean minimum movement speed between centres of activity was 0.13 m/s), and pathways frequently deviated away from the outlet river. There was no evidence of a morphological or behavioural trait or migratory pathway that distinguished successful from unsuccessful smolts. This suggests that migration movement direction in the main body of Loch Lomond appeared to be random. This was further supported by the output of a correlated random walk model which closely resembled the pathway and migration speed and distance patterns displayed by successful migrants. However, once successful smolts came within ~2 km of the lake exit, a high proportion remained in this region prior to entering the River Leven. We suggest that this “goldilocks zone” is where directional cues become apparent to migrating fish. Future studies should combine random walk models with environmental variables to determine if external factors are driving the apparently random movement patterns exhibited by smolts in lakes.
Noncommutative symplectic manifolds for self-injective Nakayama algebras
Authors: Pogorzały Zygmunt
Published: October 2021

In the paper, we study noncommutative symplectic manifolds $$(A,\llbracket \omega \rrbracket )$$

(
A
,
〚
ω
〛
)

, where A is a self-injective Nakayama algebra. There is given a full description of exact symplectic manifolds for A being the trivial extension of an hereditary Nakayama algebra.
Dynamic response of multi-degree-of-freedom systems with a Coulomb friction contact under harmonic excitation
Authors: Marino Luca , Cicirello Alice
Published: October 2021

This paper investigates the steady-state response of a harmonically excited multi-degree-of-freedom (MDOF) system with a Coulomb contact between: (1) a mass and a fixed wall; (2) two different masses; (3) a mass and an oscillating base. Although discrete MDOF models are commonly used at early design stages to analyse the dynamic performances of engineering structures, the current understanding of the friction damping effects on MDOF behaviour is still limited due to the absence of analytical solutions. In this contribution, closed-form expressions of the continuous time response, the displacement transmissibility and the phase angle of each mass of the system are derived and validated numerically for 2DOF and 5DOF systems. Moreover, the features of the analytical response are investigated, obtaining the following results: (i) the determination of the minimum amounts of friction for which the resonant peaks become finite and (ii) for which stick-slip motion can be observed at high frequencies; (iii) an equation for the evaluation of invariant points for the displacement transmissibilities; (iv) a better understanding of phenomena such as the inversions of the transmissibility curves and the onset of additional resonant peaks due to the permanent sticking of the mass in contact. All these results show that MDOF systems exhibit significantly different dynamic behaviours depending on whether the friction contact and the harmonic excitation are applied to the same or different masses.
Transcriptome analysis shows nicotinamide seed treatment alters expression of genes involved in defense and epigenetic processes in roots of seedlings of Picea abies
Authors: Laurell Cecilia , Berglund Torkel , Ohlsson Anna B.
Published: October 2021

Nicotinamide treatment of plants and plant cell cultures has been shown to promote defense and decrease levels of DNA methylation. In the present study, we used RNA-seq technology to study overall changes in gene expression induced in roots of 3-month-old spruce (Picea abies) seedlings grown from nicotinamide-treated seeds to examine the molecular mechanisms underlying the defense promotion. Approximately 350 genes were identified as differentially expressed in roots after the seed treatment. Stress response genes, including transcription factors MYB77 and LHY and two chitinase enzymes, were generally upregulated, whereas genes thought to be involved in epigenetic regulation such as DDM1, known to promote DNA methylation, were present at high frequency among the downregulated genes. Across all samples, the expression of downregulated epigenetic-related genes was highly correlated with the nicotinamide treatment, indicating a common regulation. Our results support an earlier hypothesis regarding a potential role of nicotinamide as a defense-signal mediator.
The Biology of Synovial Sarcoma: State-of-the-Art and Future Perspectives
Authors: Fiore Michele , Sambri Andrea , Spinnato Paolo , Zucchini Riccardo , Giannini Claudio , Caldari Emilia , Pirini Maria Giulia , De Paolis Massimiliano
Published: October 2021

Opinion statement

New molecular insights are being achieved in synovial sarcoma (SS) that can provide new potential diagnostic and prognostic markers as well as therapeutic targets. In particular, the advancement of research on epigenomics and gene regulation is promising. The concrete hypothesis that the pathogenesis of SS might mainly depend on the disruption of the balance of the complex interaction between epigenomic regulatory complexes and the consequences on gene expression opens interesting new perspectives. The standard of care for primary SS is wide surgical resection combined with radiation in selected cases. The role of chemotherapy is still under refinement and can be considered in patients at high risk of metastasis or in those with advanced disease. Cytotoxic chemotherapy (anthracyclines, ifosfamide, trabectedin, and pazopanib) is the treatment of choice, despite several possible side effects. Many possible drug-able targets have been identified. However, the impact of these strategies in improving SS outcome is still limited, thus making current and future research strongly needed to improve the survival of patients with SS.
Odonate fauna assemblages in the “Cansiglio Forest” (Insecta: Odonata)
Authors: Uboni Costanza , Borsato Veronica , Bacaro Giovanni
Published: October 2021

Odonata is considered a “flagship” group of insects and its investigation is of primary importance especially for protected areas where freshwater ecosystems occur. In this study, we focused on Odonate fauna in the “Cansiglio Forest” (Veneto, Italy), a karst area where the only checklist available dates back more than 40 years ago. To update the Odonate adult distribution in the area, we selected 21 ponds that were sampled monthly, from May to August, during a 2-year survey. In total, 21 species (belonging to 14 genera and 5 families) have been recorded: we confirmed 15 species from the previous species list and we added to the whole species list 6 new species. Dominant families were represented by Libellulidae (33%) and Aeshnidae (23%), the most common genus was Sympetrum (19%), and the most frequent species was Coenagrion puella (63%). In term of patterns of species richness, highly grazed and pastured ponds exhibited the lower number of species and individuals, as a probable response to the high level of animal disturbance on the vegetation and due to the eutrophication processes. Our results are important also in terms of conservation and management of freshwater sites belonging to Natura 2000 site.
Aerodynamic characteristic comparison of ultra-highly and normally loaded fans
Authors: Cao H. , Zhou Z.G , Ye X.L.
Published: October 2021

To reduce fan noise and weight, according to the structural characteristics of a turbofan engine, a fan rotor with an ultra-low rotating speed is designed in this study by using a new concept of diffusion blade profiles in which the rotating speed of an ultra-highly loaded rotor is only 0.58 times that of a normally loaded rotor. To further examine the applicability of this rotor, its matching stator is also designed. The flow fields in the ultra-highly and normally loaded fan stages are simulated using the same numerical method to conduct an aerodynamic characteristic comparison. Compared with the normally loaded rotor, the sizes of the boundary layers on the blade surfaces, the wakes behind the blades and the flow losses of the ultra-highly loaded rotor are smaller. At the design point, the efficiency of the ultra-highly loaded fan stages is higher than that of the normally loaded stage; moreover, the surge margin of the former is evidently larger than that of the latter. The ultra-highly loaded fan could be a good candidate for use in Ultra-High Bypass Ratio Geared Turbofan (UHBRGT) technology.
Development of a linear-parallel and self-adaptive under-actuated hand compensated for the four-link and sliding base mechanism
Authors: Li Jianfeng , Kong Yuan , Dong Mingjie , Jiao Ran
Published: October 2021

When the under-actuated hand pinches the object on the worktable, the trajectory of the end of the finger is not a straight line, which makes it difficult for the hand to grasp the object from its both sides. In order to overcome this shortcoming, this paper proposes a new configuration of the linear-parallel and self-adaptive under-actuated hand which uses the four-link and sliding base mechanism to compensate for the vertical displacement of the end of the finger. Based on this new configuration, the mechanical structure of the under-actuated hand is designed, which has five degrees of freedom (DOFs), and is mainly composed of two fingers, a sliding base, four link compensation mechanisms and an outer base. These two fingers have exactly the same structure and size, where each finger uses only one motor to control two joints of the finger which realizes the under-actuated function. Through the cooperation of the four-link and sliding base mechanism, the under-actuated hand can realize the linear-parallel and self-adaptive hybrid grasping mode. Kinematics analysis and contact force analysis of the under-actuated hand are discussed, and the prototype of the under-actuated hand is developed to carry out the grasping experiments. The results of the simulation and experiment all show that the under-actuated hand has good motion performance and grasping stability and can be used as an end effector for intelligent robots.
SECS Analysis of Nighttime Magnetic Perturbation Events Observed in Arctic Canada
Authors: Weygand James M. , Engebretson Mark J. , Pilipenko Viacheslav A. , Steinmetz Erik S. , Moldwin Mark B. , Connors Martin G. , Nishimura Yukitoshi , Lyons Larry R. , Russell Christopher T. , Ohtani Shin‐Ichi , Gjerloev Jesper
Published: October 2021

Large changes of the magnetic field associated with magnetic perturbation events (MPEs) with amplitudes |ΔB| of hundreds of nT and 5–10 min duration have been frequently observed within a few hours of midnight. This study compares the statistical location of nighttime MPEs with |dB/dt| ≥ 6 nT/s within the auroral current system observed during 2015 and 2017 at two stations, Cape Dorset and Kuujjuarapik, in Eastern Canada. Maps of the two dimensional nightside auroral current system were derived using the Spherical Elementary Current Systems (SECS) technique. Analyses were produced at each station for all events, and for premidnight and postmidnight subsets. We examine four MPE intervals in detail, two accompanied by auroral images, and show the varying associations between MPEs and overhead ionospheric current systems including electrojets and the field‐aligned like currents. We find 225 of 279 MPEs occurred within the westward electrojet and only 3 within the eastward electrojet. For the premidnight MPEs 100 of 230 events occurred within the Harang current system while many of the remainder occurred within either the downward region 1 current system or the upward region 2 current system. Many of the 49 postmidnight MPEs occurred in either the downward region 1 (11 events) or upward region 2 current system (27 events). These result suggest that the source of MPEs in the premidnight sector is somewhere between the inner to mid plasma sheet and the source for the MPEs in the postmidnight sector is somewhere between the inner magnetosphere and the inner plasma sheet.
Large‐Volume and Shallow Magma Intrusions in the Blackfoot Reservoir Volcanic Field (Idaho, USA)
Authors: Hastings M. S. , Connor C. B. , Wetmore P. , Malservisi R. , Connor L. J. , Rodgers M. , La Femina P. C.
Published: October 2021

The Blackfoot Reservoir volcanic field (BRVF), Idaho, USA, is a bimodal volcanic field that has hosted silicic eruptions during at least two episodes, as recently as 58 ka. Using newly collected ground and boat‐based gravity data, two large negative anomalies ( mGal) are modeled as shallow (  km) intrusions beneath a NE‐trending alignment of BRVF rhyolite domes and tuff rings. Given the trade‐off between density contrast and model volume, best‐fit gravity inversion models yield a total intrusion volume of  ; a density contrast of  kg   results in two intrusions, each  km  km and about 0.5 km thick, with cumulative volume of 100  . A network of trending faults lies directly above and on the margins of the mapped gravity anomalies. Most of these faults have  m throw; one has throw up to  m. We suggest that the emplacement of shallow sill‐like intrusions produced this fault zone and also created a ENE‐trending fault set, indicating widespread ground deformation during intrusion emplacement. The intrusions and silicic domes are located  km E of a regional, 20 mGal step in gravity. We interpret this step in gravity as thickening of the Upper Precambrian to lowermost Cambrian quartzites in the Meade thrust sheet, part of the Idaho‐Wyoming Thrust Belt. Silicic volcanism in the BRVF is a classic example of volcanotectonic interaction, influenced by regional structure and creating widespread deformation. We suggest volcanic hazard assessments should consider the possibility of large‐volume silicic eruptions in the future.
Hydroclimate Correlations Between the Alxa Desert and Adjacent Mountains in Northwestern China: Evidence From Meteorological and Tree‐Ring Data
Authors: Peng Xiaomei , Yang Bao , Xiao Shengchun , Liu Jingjing , Li Gang
Published: October 2021

It is important to understand the hydroclimate history of water‐deficient regions, such as the Alxa desert of northwest China. However, there are few high‐resolution proxy records for the hydroclimate changes over the past centuries in the Alxa desert. Fortunately, there are many tree‐ring hydroclimate reconstructions from the nearby mountains. However, the hydroclimate correlations between the Alxa desert and adjacent mountains are unclear. Here, we collected high‐quality data from 43 meteorological stations in northwest China (1960–2017). We found increasing mean temperatures in the study area, both on seasonal and annual timescales. We also found that while relative humidity had mostly decreased in the Mt. Qilian and Alxa desert, precipitation and the standardized precipitation evapotranspiration index (SPEI) increased mostly. In the Mt. Helan and Hasi ranges, relative humidity, precipitation, and SPEI all showed decreasing trends. Empirical orthogonal function analysis indicated that precipitation, relative humidity, and SPEI in the Mt. Qilian were similar to the corresponding conditions in the central and western Alxa desert. Hydroclimate conditions in the Mt. Helan and Hasi ranges were correlated with data from the eastern desert. Tree‐ring records of precipitation and SPEI from the mountains were used to validate the hydroclimate correlations during the instrumental period. We found strong similarities between hydroclimatic changes in the Mt. Qilian and those in the central and western Alxa desert, on both annual and decadal timescales, from 1877 to 2006. Tree‐ring width chronologies from mountainous areas were shown to faithfully represent the hydroclimatic history of the desert area.
Doxorubicin-induced novel circRNA_0004674 facilitates osteosarcoma progression and chemoresistance by upregulating MCL1 through miR-142-5p
Authors: Ma Xiao-Long , Zhan Tai-Cheng , Hu Jian-Ping , Zhang Chun-Lin , Zhu Kun-Peng
Published: October 2021

Accumulating evidence has shown that circular RNA (circRNA) dysregulation is involved in various types of cancer, including osteosarcoma (OS). Nevertheless, the role and mechanism of circRNAs in OS progression and chemoresistance remain elusive. We found that a novel doxorubicin-induced circular RNA, hsa_circ_0004674, screened by whole total transcriptome RNA sequencing in our previous study, was upregulated in OS chemoresistant cell lines and tissues and also connected with patients’ poor prognosis. Circ_0004674 knockdown remarkably suppressed OS cell chemoresistance, proliferation, migration, invasion, OS tumor growth, and enhanced cell cycle arrest and apoptosis in vitro and in vivo through control the expression of the antiapoptotic protein MCL1, a member of the Bcl-2 gene family. Further online bioinformatics analysis revealed that miR-142-5p had potential binding sites that can bind circ_0004674 and the 3′UTR of MCL1 mRNA. Moreover, the expression and function of miR-142-5p were conversely correlated with circ_0004674 in vitro. RIP, pull-down, luciferase assay, and RNA FISH demonstrated that circ_0004674 could compete with MCL1 for miR-142-5p binding to counteract miR-142-5p-mediated repression of MCL1 at the post-transcriptional level. To sum up, our study sheds light on the critical role of the oncogenic circ_0004674/miR-142-5p/MCL1 axis in OS progression and chemoresistance, providing a novel potential target for OS therapy.
Cell death inhibitors protect against brain damage caused by cardiac ischemia/reperfusion injury
Authors: Liao Suchan , Apaijai Nattayaporn , Luo Ying , Wu Jun , Chunchai Titikorn , Singhanat Kodchanan , Arunsak Busarin , Benjanuwattra Juthipong , Chattipakorn Nipon , Chattipakorn Siriporn C.
Published: October 2021

Cognitive impairment has been reported in patients with myocardial infarction despite a successful reperfusion therapy. Several modes of cell death are involved in brain damage during cardiac ischemia/reperfusion (I/R) injury. Although apoptosis, necroptosis, and ferroptosis inhibitors provided neuroprotection against cerebral I/R injury, the effects of these cell death inhibitors on the brain following cardiac I/R injury have never been investigated. We hypothesized that apoptosis, necroptosis, and ferroptosis inhibitors attenuate brain damage following cardiac I/R injury. One-hundred and twenty-six male rats were used: 6 rats were assigned to sham operation and 120 rats were subjected to 30-min regional cardiac ischemia and 120-min reperfusion. Rats in cardiac I/R group were pretreated with either vehicle (n = 12) or one of cell death inhibitors. Rats treated with apoptosis, necroptosis, or ferroptosis inhibitor were subdivided into three different doses including low (L), medium (M), and high (H) doses (n = 12/group). Z-VAD, necrostatin-1 (Nec-1), and ferrostatin-1 (Fer-1) were used as apoptosis, necroptosis, and ferroptosis inhibitor, respectively. Rats were sacrificed at the end of reperfusion, and the brain was used to analyze dendritic spine density, Alzheimer’s disease (AD)-related proteins, blood–brain barrier (BBB) tight junction proteins, mitochondrial function, inflammation, and cell death. Our data showed that cardiac I/R led to brain damage and only apoptosis occurred in the hippocampus after cardiac I/R injury. In the cardiac I/R group, treatment with M-Z-VAD and all doses of Nec-1 decreased hippocampal apoptosis and amyloid beta aggregation, thereby reducing dendritic spine loss. M- and H-Fer-1 also reduced dendritic spine loss by suppressing ACSL4, TNF-α, amyloid beta, and tau hyperphosphorylation. Moreover, Bax/Bcl-2 was decreased in all treatment regimen except L-Z-VAD. Additionally, M-Z-VAD and M-Fer-1 partially attenuated mitochondrial dysfunction. Only L-Nec-1 preserved BBB proteins. In conclusion, cell death inhibitors prevented hippocampal dendritic spine loss caused by cardiac I/R injury through different mechanisms.
HES5-mediated repression of LIGHT transcription may contribute to apoptosis in hepatocytes
Authors: Miao Xiulian , Guo Yan , Zeng Sheng , Liu Xingyu , Teng Xiao , Li Luyang , Hong Wenxuan
Published: October 2021

Non-alcoholic fatty liver disease (NAFLD) is prototypical form of metabolic syndrome and has become a global pandemic. Hepatocytes undergo apoptosis in the pathogenesis of NAFLD. We report that the lymphokine LIGHT/TNFSF14 was upregulated in the murine NAFLD livers and in hepatocytes treated with free fatty acids (palmitate, PA). LIGHT knockdown or neutralization attenuated PA-induced apoptosis of hepatocytes. Similarly, knockdown or blockade of LTβR, the receptor for LIGHT, ameliorated apoptosis in hepatocytes exposed to PA. Ingenuity pathway analysis (IPA) revealed several Notch-related transcription factors as upstream regulators of LIGHT, of which HES5 expression was downregulated paralleling LIGHT induction in the pathogenesis of NAFLD. HES5 knockdown enhanced whereas HES5 over-expression weakened LIGHT induction in hepatocytes. HES5 was found to directly bind to the LIGHT promoter and repress LIGHT transcription. Mechanistically, HES5 interacted with SIRT1 to deacetylate histone H3/H4 on the LIGHT promoter to repress LIGHT transcription. SIRT1 knockdown or inhibition offset the effect of HES5 over-expression on LIGHT transcription and hepatocyte apoptosis. In conclusion, our data unveil a novel mechanism that might contribute to excessive apoptosis in hepatocyte exposed to free fatty acids.
Label-free sensing of virus-like particles below the sub-diffraction limit by wide-field photon state parametric imaging of a gold nanodot array
Authors: Jin Xiao , Zhang Heng , Ni Bin , Liu Weiping , Hou Lianping , Marsh John H. , Ye Shengwei , Sun Xiao , Li Xiaofeng , Li Shanhu , Dong Lei , Hou Jamie Jiangmin , Sun Ming , Xu Bin , Xiong Jichuan , Liu Xuefeng
Published: October 2021

In this work we use a parallel four-quadrant sensing gold nanodot array to sense virus-like particles with a sub-diffraction limit size in a wide-field image. The label-free sensing of viruses enables the high-throughput onsite screening of viruses.
Use of hydrogen storage in an off-grid system for implementing a renewable-energy system
Authors: Noro Yasuhiro , Uchiyama Satoshi
Published: October 2021

This paper presents the purpose, advantages, system constitution, operation method and estimation results of using hydrogen storage in a small-scale electric power (off-grid) system when renewable energy sources are introduced. It is assumed that all electricity is supplied from renewable energy sources without using a diesel generator that requires fossil fuels. In other words, for a small-scale electric power system on a remote island, a power-supply system comprising a photovoltaic power-generation system, storage battery, hydrogen-production system, storage tank and a fuel cell is considered. The condition under which the total construction cost is minimized by considering the equipment capacity, operation method of the battery and fuel cell as parameters is investigated. The results demonstrate that the construction cost can be reduced by applying the method of surplus magnification and hydrogen storage. The initial cost is ~70% when hydrogen storage is added as compared with the case in which a storage battery alone is used. Such a cost-effective system can contribute to Japan’s self-reliance with regard to the energy sector in the future.
An unusual presentation of non-infectious ascending aortitis: a case report of an asymptomatic murmur
Authors: Calhoun Avery E , Bilen Ozlem
Published: October 2021

Background

Aortitis refers to pathologic inflammation of the aortic wall and is broadly categorized into inflammatory (or non-infectious) and infectious aortitis. While rare, isolated non-infectious ascending aortitis (I-NIAA) is a clinical entity that is becoming increasingly recognized but remains poorly understood.

Case summary

A 72-year-old man presented with an asymptomatic murmur and was found to have severe aortic insufficiency second to a large ascending aortic aneurysm. He underwent surgical repair and pathology revealed isolated non-infectious ascending aortitis. Following successful surgical repair, he developed joint pains which were successfully treated with glucocorticoids.

Discussion

Isolated non-infectious aortitis is a rare entity that warrants further investigation. This case highlights the importance of sending surgical specimens for histopathologic evaluation even when a systemic process is not evident at the time of surgical repair. The development of systemic symptoms following surgical repair in this patient emphasizes the importance of thorough rheumatologic evaluation in patients found to have I-NIAA. Isolated non-infectious ascending aortitis remains poorly understood, and further study is needed to evaluate both its existence as a distinct clinical entity and the role of immunosuppressive therapy.
This and that: management of Tetralogy of Fallot and pulmonary vein stenosis in an infant—a case report
Authors: Scott Christopher P , Shibbani Kamel , Caldarone Christopher A , McLennan Daniel
Published: October 2021

Background

Tetralogy of Fallot (TOF) is the most common congenital cyanotic cardiac lesion. Pulmonary vein stenosis occurs much less commonly and remains difficult to manage. It is exceedingly uncommon for a patient to have both lesions. This case highlights the diagnostic and management difficulties in an infant with these two lesions.

Case summary

The patient is a 4-month-old female infant with a history of TOF status post right ventricular outflow tract stent placement who presented after a hypoxaemic event at home to 40% SpO2. Computed tomography angiography demonstrated previously undiagnosed pulmonary vein stenosis of all four veins. She underwent multiple catheter-based palliations including balloon dilations and stent placements in each pulmonary vein in order to maximize her chances of successful definitive repair. She underwent successful repair of her TOF and pulmonary vein stenosis at ten months of age.

Discussion

The combination of TOF and pulmonary vein stenosis is not common, but when these lesions co-occur, they present a significant dilemma in determining the timing of surgery in order to optimize the odds of a successful outcome. This case demonstrates that serial catheter-based procedures can be valuable tools in minimizing pre-operative risk factors and highlights one strategy in determining timing of definitive surgical repair.
Type E coronary artery dissection caused by intravascular lithotripsy balloon rupture; vessel anatomy and characteristics in a lithoplasty complication case as detailed by optical coherence tomography: a case report
Authors: Lee Tjen Jhung , Wan Rahimi Wan Faizal Bin , Low Ming Yoong , Nurruddin Amin Ariff
Published: October 2021

Background

Intravascular lithotripsy is a new method used to treat calcified coronary lesions (CCLs). Percutaneous coronary intervention of CCLs has conventionally been classified as a complex procedure. In the majority of calcified cases, atherectomy is required for sufficient plaque modification prior to stent implantation. Intravascular lithotripsy has been shown to be safe and effective in clinical trials, but as worldwide usage increases, cases of complications are beginning to emerge.

Case summary

We describe a 71-year-old woman, who after an episode of non-ST-elevation acute coronary syndrome underwent coronary angiography. The culprit vessel was identified to be a severely stenosed left anterior descending artery which was also heavily calcified and tortuous. Intravascular lithotripsy (IVL) was employed for calcium modification prior to stent implantation, but the IVL balloon ruptured during shockwave lithotripsy, resulting in coronary artery dissection. Subsequent management steps and stent deployment resulted in favourable angiographic results. Our findings are further detailed on optical coherence tomography, demonstrating certain features which might predispose to IVL balloon rupture.

Discussion

We discuss the mechanism of action during intravascular lithotripsy, and how the shockwaves from the lithotripter modify calcified lesions, whilst keeping soft tissue unharmed. Results from clinical trials and multiple real-world studies have shown that complication rates are low. This case report aims to illustrate how the rupture of an intravascular lithotripsy balloon can result in coronary artery dissection. Optical coherence tomography can help in identifying potential anatomical features which may precede such complications.
Targeting the methyltransferase setd7 prevents myocardial ischemic injury: a translational study
Authors: Ambrosini S , Montecucco F , Kolijn D , Akhmedov A , Pedicino D , Mohammed S , Kiss A , Beltrami A , Crea F , Luescher T , Hamdani N , Costantino S , Paneni F
Published: October 2021

Background

Despite appropriate revascularization strategies, a significant number of patients with myocardial infarction (MI) develop ischemic heart failure suggesting that breakthrough therapies are yet to be approved in this setting. Methylation of non-histone proteins is emerging as a central regulatory mechanism in health and disease. The methyltransferase SETD7 has shown to methylate and alter the function of a variety of proteins in vitro, however its function in the heart is poorly understood.

Purpose

In the present study we sought to determine the role of SETD7 in myocardial ischemic injury.

Methods

Neonatal rat ventricular myocytes (NRVM) were exposed to glucose deprivation (GD) for 15 h, in the presence of the selective SETD7 inhibitor [(R)-PFI-2] or its inactive enantiomer [(S)-PFI-2]. Western blot and real time PCR were employed to investigate the effects of energy stress on SETD7 and the Hippo pathway, while apoptosis and oxidative stress were assessed by Caspase-3 activity assay and mitochondrial swelling. YAP activity was assessed through chromatin immunoprecipitation assay (ChIP), its localization was examined by confocal microscopy while mono-methylation was assessed by immunoblotting. Expression of YAP-dependent antioxidant genes was assessed by western blot. SETD7 knockout (SETD7−/−) mice and wild-type (WT) littermates underwent ischemia/reperfusion (I/R) injury. Rats underwent permanent ligation of left anterior descending coronary artery (MI). Left ventricular (LV) myocardial samples were collected from mice undergoing I/R injury and patients with ischemic cardiomyopathy (ICM) and treated ex-vivo with (R)-PFI-2. SETD7 and antioxidant genes expression was assessed in peripheral blood mononuclear cells (PBMCs) from patients with ST-elevation MI (STEMI).

Results

We show that SETD7 is activated upon energy deprivation in cultured NRVMs and methylates the Hippo pathway effector YAP, leading to its cytosolic retention and impaired transcription of antioxidant genes. Pharmacological inhibition of SETD7 by (R)-PFI-2 restored YAP nuclear localization thus preventing mitochondrial reactive oxygen species (mtROS) and apoptosis. SETD7 deletion in mice attenuated I/R injury, mtROS and LV dysfunction by restoring YAP-dependent transcriptional programs. SETD7/YAP dysregulation was also observed in rats with MI and LV specimens from ICM patients. Of note, (R)-PFI-2 treatment prevented titin oxidation and myofilament stiffness in cardiomyocytes isolated from I/R mice and patients with ICM. Finally, SETD7 was upregulated in STEMI patients and its expression negatively correlated with antioxidant genes.

Conclusions

Targeting SETD7 may represent a valid therapeutic strategy to protect the heart during ischemia.

Funding Acknowledgement

Type of funding sources: Public Institution(s). Main funding source(s): University of Zurich
Posterior Tibial Tendon Transfer for Drop Foot With an Accessory Navicular
Authors: Yergensen Christopher J. , Kress Kristin , Jaffe David
Published: October 2021

Posterior tibial tendon transfer for chronic drop foot deformity can be performed to reduce a patient’s dependence on an ankle-foot orthosis. Obtaining adequate length of the posterior tibial tendon at harvest is critical for having a successful surgical outcome. The presence of an accessory navicular could limit the length of tendon available for transfer. The technique for performing a transfer of the posterior tibial tendon through the interosseous membrane in the setting of an accessory navicular is reported. Using the accessory bone to secure the transfer can lead to rapid transfer incorporation and a successful outcome.

Level of Evidence: Diagnostic Level V—case report and technique guide. See Instructions for Authors for a complete description of levels of evidence.
Automation in Busulfan Therapeutic Drug Monitoring: Evaluation of an Immunoassay on two Routine Chemistry Analyzers
Authors: Verougstraete Nick , Stove Veronique , Verstraete Alain G. , Oyaert Matthijs
Published: October 2021

Background:

Therapeutic drug monitoring (TDM) of busulfan is recommended for hematopoietic stem cell transplant recipients. Timely reporting of these TDM results is essential given the short administration period and the planned dose adjustments on day 2. The authors evaluated the performance of a new nanoparticle-based competitive immunoassay on two routine clinical chemistry analyzers and compared its performance to that of an in-house high-resolution mass spectrometry (HRMS) method.

Methods:

The MyCare Oncology Busulfan Assay Kit (Saladax Biomedical) was applied on two routine clinical chemistry analyzers (Abbott Architect c8000 and Roche Cobas c502) with a linearity range of 187–2000 ng/mL. The study evaluation measured imprecision and accuracy, sample probe carry-over, and dilution integrity. Method comparison with liquid chromatography (LC)-HRMS was performed using samples from patients undergoing busulfan treatment.

Results:

Within- and between-run coefficient of variations for both analyzers were ≤5.23% and ≤8.45%, respectively, across the busulfan concentration range. The obtained biases were ≤10.3%. Both analyzers met the acceptance criteria for sample probe carry-over and dilution integrity. Agreement between the immunoassay and LC-HRMS was high: 92% and 89% of the samples measured on Architect and Cobas, respectively, were within the ±15% limit compared to the corresponding LC-HRMS results.

Conclusions:

Overall, good analytical performance and high agreement with LC-HRMS results were obtained for the immunoassay installed on both routine clinical chemistry analyzers. Therefore, this assay could be implemented as a valid alternative for LC methods in clinical laboratories on different open-channel clinical chemistry analyzers, resulting in shorter turn-around times for reporting busulfan TDM results with subsequent faster dosage adjustments.
Lacrimal Gland Hypoplasia and Corneal Anesthesia in MIRAGE Syndrome: A Case Report and Literature Review
Authors: Basilious Amy , Basilious Alfred , ElJalbout Ramy , Robert Marie-Claude
Published: October 2021

Purpose:

The purpose of this report was to describe the ocular findings in Myelodysplasia, Infection, Restriction of growth, Adrenal hypoplasia, Genital problems, and Enteropathy (MIRAGE) syndrome, a multisystem congenital disorder.

Methods:

This was a case report and literature review.

Results:

An infant with MIRAGE syndrome (combined immunodeficiency with recurrent infections, growth restriction, adrenal insufficiency, 46,XY karyotype with hypovirilization, dysphagia, gastroesophageal reflux disease, and dysautonomia) underwent ophthalmological evaluation because of persistent conjunctivitis during his 8-month admission in the neonatal intensive care unit. His parents noted absence of tears when crying since birth. Bilateral broad corneal epithelial defects were noted, and treatment was initiated with frequent lubricating ointment. At 9 months, his vision was estimated at 20/380 in both eyes using Teller Acuity Cards. There were persistent bilateral epithelial defects, confluent punctate epithelial erosions, low Schirmer strip wetting (right eye 3 mm and left eye 2 mm), and decreased corneal sensation. Brain magnetic resonance imaging images demonstrated hypoplastic lacrimal glands bilaterally. More aggressive lubrication and installation of punctal plugs in all 4 lids were successful at preventing further epithelial defects.

Conclusions:

This case presents deficient lacrimation as a manifestation of MIRAGE syndrome and is the first to identify lacrimal gland hypoplasia and corneal anesthesia. Autonomic and neurologic dysfunction have been proposed to play a role in the pathophysiology of hypolacrimation in similar syndromes and likely contributed to the poor ocular surface in this case. Patients with MIRAGE should undergo ophthalmic assessment as soon as possible after birth because early intervention is essential to preventing irreversible corneal damage.
Heritability of musculoskeletal pain and pain sensitivity phenotypes: 2 generations of the Raine Study
Authors: Waller Rob , Melton Philllip E. , Kendell Michelle , Hellings Sophie , Hole Erlend , Slevin Alison , Soares Jian , Jacques Angela , Straker Leon , Beales Darren
Published: October 2021

There is a need to better understand biological factors that increase the risk of persistent musculoskeletal (MSK) pain and heightened pain sensitivity. Knowing the heritability (how genes account for differences in people's traits) can enhance the understanding of genetic vs environmental influences of pain and pain sensitivity. However, there are gaps in current knowledge, including the need for intergenerational studies to broaden our understanding of the genetic basis of pain. Data from Gen1 and Gen2 of the Raine Study were used to investigate the heritability of MSK pain and pressure and cold pain sensitivity. Participants included parents (Gen1, n = 1092) and their offspring (Gen2, n = 688) who underwent a battery of testing and questionnaires including pressure and cold pain threshold testing and assessments of physical activity, sleep, MSK pain, mental health, and adiposity. Heritability estimates were derived using the Sequential Oligogenic Linkage Analysis Routines software. Heritability estimates for MSK pain and pressure pain sensitivity were significant, accounting for between 0.190 and 0.289 of the variation in the phenotype. By contrast, heritability of cold pain sensitivity was not significant. This is the largest intergenerational study to date to comprehensively investigate the heritability of both MSK pain and pain sensitivity, using robust statistical analysis. This study provides support for the heritability of MSK pain and pain sensitivity to pressure, suggesting the need for further convergence of genetic and environmental factors in models for the development or maintenance of these pain disorders.
Transcortical approach surgery versus external ventricular drainage in treating intraventricular hemorrhage
Authors: Su Jiahao , Xing Yichun , Liang Sitao , Lin Qichang , Liu Huijiao
Published: October 2021

Intraventricular hemorrhage is a serious intracerebral hemorrhagic disease with high mortality and poor prognosis. This retrospective study designed to investigate the therapeutic effect of transcortical approach surgery versus extraventricular drainage (EVD) on patients with intraventricular hemorrhage.

Patients with intraventricular hemorrhage in Zhongshan City People's Hospital from January 01, 2014 to June 01, 2019 were retrospectively examined. They were divided into transcortical approach surgery groups and EVD groups to analyze the clinical characteristics and prognosis.

A total of 96 patients were enrolled in the study (24 in the transcortical approach surgery group and 72 in the EVD group). The efficiency of postoperative operation was 15/19 in the transcortical approach surgery group and 24/48 in the EVD group (P = .012). The Glasgow Outcome Scale was 3.63 ± 1.27 in the transcortical approach surgery group and 2.80 ± 1.87 in the EVD group (P = .049). The postoperative residual blood volume was 9.62 ± 3.64 mL in the transcortical approach surgery group and 33.60 ± 3.53 mL in the EVD group (P < .001). The incidence of hydrocephalus after the operation was 1/23 in the transcortical approach surgery group and 19/53 in the EVD group. The 30-day postoperative mortality was 16/56 in the EVD group and 1/23 in the transcortical approach surgery group. The transcortical approach surgery group was significantly better compared with the EVD group.

This study showed that the transcortical approach for ventricular hemorrhage compared with EVD improved the hematoma clearance rate, shortened catheterization time, reduced the incidence of postoperative hydrocephalus, decreased patient mortality, led to a better prognosis, and reduced complications of hydrocephalus.