bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Ramadan intermittent fasting on gut microbiome: is the diet key?
Authors: Saglam Duygu , Colak Gozde Aritici , Sahin Eray , Ekren Berkay Yekta , Sezerman Ugur , Bas Murat
Published: August 2023

Much research has been conducted regarding the impact of diet on the gut microbiota. However, the effects of dietary habits such as intermittent fasting are unclear. This study aimed to investigate the effect of intermittent fasting during Ramadan on the gut microbiota. The study was conducted on 12 healthy adult individuals who practiced fasting 17 h per day for 29 consecutive days during the month of Ramadan. To determine the dietary intake of individuals, a 3-day dietary record was kept at the beginning and end of the study. Reads that passed quality filtering were clustered, and custom-prepared 16S rRNA gene regions of bacteria associated with the human microbiome were used as a reference. Consensus sequences were created, and genus-level taxonomic annotations were determined using a sequence identity threshold of 95%. The correlations between the dietary intake measurements of the participants and the respective relative abundance of bacterial genera were investigated. The results showed that Firmicutes were higher in abundance in the gut microbiota before fasting among participants, while they were significantly lower in abundance at the end of Ramadan fasting (p < 0.05). Proteobacteria were significantly higher in abundance at the end of the month of Ramadan (p < 0.05). Fasting was associated with a significant decrease in levels of seven genera: Blautia, Coprococcus, Dorea, Faecalicatena, Fusicatenibacter, Lachnoclostridium, and Mediterraneibacter. Conversely, the abundances of two bacterial genera were enhanced at the end of the fasting month: Escherichia and Shigella. The results of the dietary intake analysis showed that a negative correlation was detected for three comparisons: Ihubacter and protein (rho = −0.54, p = 0.0068), Fusicatenibacter and vegetables (rho = −0.54, p = 0.0042), and Intestinibacter and nuts (rho = −0.54, p-value = 0.0065). The results suggest that even when the fasting period during Ramadan is consistent, the types of food consumed by individuals can affect the gut microbiota.
Phenotypes in children with GNAO1 encephalopathy in China
Authors: Li Yanmei , Chen Hong , Li Lin , Cao Xueyan , Ding Xin , Chen Li , Cao Dezhi
Published: August 2023

Background

The GNAO1 gene encodes the α-subunit (Gαo) of the heterotrimeric guanine nucleotide-binding protein (G protein). The aim of this study was to explore the clinical characteristics of patients with GNAO1 pathogenic variations.

Methods

Ten patients with pathogenic variations in GNAO1 were enrolled from the Shenzhen Children's Hospital. Clinical data from several cases previously reported from China were also included and analyzed.

Results

Twenty-seven patients with variations in GNAO1 were analyzed (10 patients from Shenzhen Children's Hospital, 17 patients from previously published studies) including 12 boys and 15 girls. The median age of onset was 3 months with moderate to severe global developmental delay. Nineteen different GNAO1 heterozygous variants were identified. Epilepsy was observed in 18 patients (67%, 18/27), movement disorder (MD) was observed in 22 patients (81%, 22/27), and both were seen in 13 patients (48%, 13/27). Seizures typically presented as focal seizures in all patients with epilepsy. MD typically presented as dystonia and chorea. Loss-of-function (LOF) or partial loss-of-function (PLOF) mutations were more frequent in patients with developmental and epileptic encephalopathy (p = 0.029). Interictal electroencephalograms showed multifocal or diffuse epileptiform discharges. The most common magnetic resonance imaging finding was widened extracerebral space. In contrast to MD, in which improvements were not common, seizures were easily controlled by anti-seizure medications. Severe dystonia in three patients was effectively treated by deep brain stimulation. Seven (26%, 7/27) patients died of respiratory complications, status dystonicus, choreoathetosis, or sudden unexpected death in epilepsy.

Conclusion

We analyzed clinical data of 27 cases of GNAO1-related encephalopathy in China. MD seemed to be the central feature and was most difficult to control. LOF or PLOF variants were significantly associated with developmental and epileptic encephalopathy. The active intervention of severe dystonia may prevent death due to status dystonicus. However, future studies with larger samples are needed to confirm these results.
The CRAVE and ARGE scales for motivation states for physical activity and sedentarism: Brazilian Portuguese translation and single-item versions
Authors: Filgueiras Alberto , Stults-Kolehmainen Matthew A. , Boullosa Daniel , Sinha Rajita , Bartholomew John B. , McKee Paul , Gilson Todd A. , Keegan Richard , Viana Artur , Bueno Fabio Amador , Medeiros André Ricarte , Militão-de-Leutério Sofia F. , Ash Garrett I.
Published: August 2023

Motivation states for physical activity and sedentarism potentially vary from moment to moment. The CRAVE scale (Cravings for Rest and Volitional Energy Expenditure) was developed to assess transient wants and desires to move. Three studies were conducted with the aims of: (1) translating and validating the scale in Brazilian Portuguese, (2) examining changes with exercise, and (3) determining the best single-item for Move and Rest subscales for English and Portuguese. In Study 1, six bilingual speakers translated the scale into Brazilian Portuguese [named Anseios por Repouso e Gastos com Energia (ARGE)]. The ARGE had good content validity coefficients across three dimensions (0.89–0.91), as determined by three independent, bilingual referees. 1,168 participants (mean age = 30.6, SD = 12.2) from across Brazil completed an online version of the ARGE. An Exploratory Factor Analysis found two clear, oblique, and inversely related factors (Move and Rest; GFI = 1.00, RMSR = 0.03). Reliability was good (Cronbach α’s: 0.93 and 0.92). Two models of the scale (10 vs. 13 items) were compared with Confirmatory Factor Analysis. The previously validated version using 10 scored items (GFI = 1.00, RMSEA = 0.07, RMSR = 0.02) outperformed the version scored with 13 items. State anxiety and exercise behavior had small associations with Move and Rest (−0.20 to 0.26). In Study 2, ARGE Move scores had high correspondence post-session (ICC = 0.83) for 9 women performing short Sprint Interval Training (sSIT; 6 sessions). Large, but non-significant, effects were detected for changes in motivation states with sSIT. In Study 3, IRT analyses found that for the United States sample, “be physically active” and “be still” were the most representative items for Move and Rest, respectively, while for the Brazil sample they were “exert my muscles” and “be a couch potato.” Overall, it was found that: (A) the ARGE scale demonstrated good psychometric properties, (B) the original scoring (with 10 items) resulted in the best model, (C) it had small associations with exercise behavior, and (D) the subscales were reduced to single items that varied by country, indicating potential cultural differences in the concept of motivation states for physical activity.
A social network analysis: mental health scales used during the COVID-19 pandemic
Authors: Chen Shuang , Lan Xue , Yu Han
Published: August 2023

Introduction

The focus on psychological issues during COVID-19 has led to the development of large surveys that involve the use of mental health scales. Numerous mental health measurements are available; choosing the appropriate measurement is crucial.

Methods

A rule-based named entity recognition was used to recognize entities of mental health scales that occur in the articles from PubMed. The co-occurrence networks of mental health scales and Medical Subject Headings (MeSH) terms were constructed by Gephi.

Results

Five types of MeSH terms were filtered, including research objects, research topics, research methods, countries/regions, and factors. Seventy-eight mental health scales were discovered.

Discussion

The findings provide insights on the scales used most often during the pandemic, the key instruments used to measure healthcare workers’ physical and mental health, the scales most often utilized for assessing maternal mental health, the tools used most commonly for assessing older adults’ psychological resilience and loneliness, and new COVID-19 mental health scales. Future studies may use these findings as a guiding reference and compass.
Self-reported low self-esteem due to poor maternal care improves with the existential approach in bipolar disorder: a case report
Authors: Hirakawa Hirofumi , Terao Takeshi , Kawano Nobuko
Published: August 2023

Parental nurturing attitudes influence children and are linked to the establishment of self-esteem. Females who have experienced poor maternal care during their childhood may have low self-esteem, and this factor may significantly augment the likelihood of depression. Particularly, childhood maltreatment among individuals with bipolar disorder is associated with unfavorable clinical features, such as a heightened risk of severe manic, depressive, or psychotic symptoms, as well as suicide attempts. Here, we report a case of a woman with bipolar disorder who had self-reported low self-esteem due to poor maternal care, which subsequently improved via an existential approach. This existential approach confers meaning to the lives of every individual, even in the face of adversity. Our findings suggest that the existential approach may enable the discovery of more positive life values during times of hardship and could improve self-reported low self-esteem due to poor maternal and change the way of life in patients with bipolar disorder.
Prevalence of insulin resistance in Chinese solar greenhouse and field workers: evidence from a solar greenhouse and field workers study
Authors: Yan Tenglong , Wang Minghui , Yang Siwen , Wang Yuqian , Zhou Xingfan , Zhu Xiaojun , Ma Wenjun , Tang Shichuan , Li Jue
Published: August 2023

Evidence suggests that agricultural workers are at higher risk of insulin resistance (IR), but few studies have investigated IR in solar greenhouse workers, who are exposed to higher concentrations of agricultural risk factors than traditional agricultural workers. A prevalence study was conducted in a greenhouse vegetable farm in China. In total, 948 participants were enrolled in this study. Among them, 721 participants were allocated to the greenhouse worker group (G group), and 227 participants were assigned to the field worker group (F group). The TyG index, which is an indicator to evaluate prediabetes (IR), was calculated by the formula: TyG index = ln [fasting triglycerides (mg/dL) × fasting plasma glucose (mg/dL)/2]. To evaluate the associations of TyG index alternation with solar greenhouse and field work, multiple linear regression (MLR) and logistic regression models were performed. The TyG index in the G group (8.53 ± 0.56) was higher than that in the F group (8.44 ± 0.59) (p < 0.05). Solar greenhouse work was positively associated with an increased TyG index in both the multiple linear regression model [β = 0.207, (0.006, 0.408)] and the logistic regression model [OR = 1.469, (1.070, 2.016)]. IR was associated with the solar greenhouse work. However, the determination of agricultural hazard factors needs to be further strengthened to improve exposure assessment.

Graphical Abstract
What Happens To Astronauts’ Brains When They Travel To Space?
Authors: Hupfeld Kathleen E. , McGregor Heather R. , Tays Grant D. , Seidler Rachael D.
Published: August 2023

For over 20 years, astronauts have lived and worked aboard the International Space Station. Astronauts face many challenges living in space, like not having Earth’s gravity. This means that astronauts do everything—from brushing their teeth to doing science experiments—while floating. Not having Earth’s gravity makes everything more difficult, and it changes astronauts’ brains. Over the last decade, we tested 15 astronauts before and after their space travel. We measured their walking, balance, and coordination, and collected pictures of their brains. This article talks about our results. We found that, when astronauts returned to Earth, they had problems moving, like trouble walking and balancing. We also found that spaceflight changed how astronauts’ brains look and function. We finish our article by talking about what is still left to learn. Our big goal is to keep astronauts healthy for very long missions—to Mars and beyond!
Core decompression vs. allogenic non-vascularized bone grafting in patients with osteonecrosis of the femoral head
Authors: Mei Jin , Jiang Zai-ping , Pang Li-li , Huang Yingtao , Gong Yu , Zhu Jun , Zhang Ling-wei
Published: August 2023

Background

Core decompression and allogenic non-vascularized bone grafting are used in the early stage of osteonecrosis of the femoral head for a period. Since the comparison of the core decompression and allogenic non-vascularized bone grafting are less reported, the purpose of our study was to investigate the difference of two procedures in patients with the osteonecrosis of the femoral head.

Methods

Between January 2018 and January 2019, 59 patients (64 hips) were divided into core decompression group and non-vascularized bone grafting group according to their procedures. The primary outcomes are visual analog score (VAS) and Harris hip score. Survivorship was analyzed with the collapse of the femoral head or conversion to total hip arthroplasty (THA) as the endpoint.

Results

At the final follow-up, two hips underwent THA in the core decompression group and three hips in the allogenic non-vascularized bone grafting group. The radiographic survival rates were 76.9% and 77.3%, respectively, in both groups. The VAS of the core decompression group was 6.08 ± 1.164 and 3.30 ± 1.431 before and 2 years after operation (P < 0.05), respectively. The VAS of the allogenic non-vascularized bone grafting group was 6.00 ± 1.209 and 3.15 ± 1.537 before and 2 years after operation (P < 0.05), respectively. The Harris hip score of the core decompression group was 52.49 ± 6.496 before operation, and 2 years after operation, it increased by 81.14 ± 8.548 (P < 0.05); The Harris hip score of allogenic the non-vascularized bone grafting group was 53.56 ± 5.925 and 81.33 ± 7.243 before and 2 years after operation (P < 0.05), respectively. In the core decompression group, body mass index (BMI) >25 kg/m2 was correlated with the collapse of femoral head or conversion to THA [P < 0.05; 95% confidence interval (CI), 0.006–1.334], and Association Research Circulation Osseous (ARCO) III was correlated with the collapse of femoral head or conversion to THA (P < 0.05; 95% CI, 2.514–809.650). In the allogenic non-vascularized bone grafting group, age, BMI, and ARCO stage were significantly associated with the collapse of femoral head or conversion to THA (P > 0.05).

Conclusion

The clinical survival rate of the femoral head in the core decompression group was slightly better than that in the allogenic non-vascularized bone grafting group. There was no significant difference in the radiographic survival rate of the femoral head between the two groups. Both groups can alleviate pain and improve functional of patients, but there was no significant difference in the degree of improvement. In the core decompression group, BMI >25 kg/m2 and ARCO III correlated with the collapse of femoral head or conversion to THA. In the allogenic non-vascularized bone grafting group, no association was found between age, BMI, and ARCO stage and the collapse of femoral head or conversion to THA.

Level of evidence

III.
Storage Temperature and Grain Moisture Effects on Market and End Use Properties of Red Lentil
Authors: Bhattarai Bhawana , Walker Cassandra K. , Wallace Ashley J. , Nuttall James G. , Hepworth Graham , Panozzo Joe F. , Partington Debra L. , Fitzgerald Glenn J.
Published: August 2023

Storing lentil is a strategy used by growers to manage price volatility. However, studies investigating the impact of storage conditions on the market and end use properties of lentil are limited. This study examined the effects of storage temperature (4, 15, 25, and 35 °C) and grain moisture (10 and 14%, w/w) on traits related to market (seed coat colour), viability (germination capacity), and end use properties (hydration capacity, milling efficiency, and cooking quality) in four red lentil cultivars (PBA Bolt, PBA Hallmark, PBA Hurricane, PBA Jumbo2) over 360 days. Storing lentil at 14% moisture content and 35 °C significantly (p = 0.05) darkened seed coat after 30 days, caused complete loss of viability within 180 days and reduced cooking quality (cooked firmness) after 120 days across all tested cultivars. Storing lentil at 10% moisture content and 35 °C reduced hydration capacity after 30 days, and milling efficiency after 120 days across all cultivars tested. PBA Jumbo2 exhibited a higher rate of degradation in hydration capacity and cooking quality, and a lower rate of degradation in the other traits studied. Storing lentil at ≤15 °C prevented degradation of all quality traits. These findings will support improved lentil storage protocols to maintain quality and improve economic outcomes for the pulse industry.
Combined Effects of Straw Return with Nitrogen Fertilizer on Leaf Ion Balance, Photosynthetic Capacity, and Rice Yield in Saline-Sodic Paddy Fields
Authors: Dang Kun , Ran Cheng , Tian Hao , Gao Dapeng , Mu Jinmeng , Zhang Zhenyu , Geng Yanqiu , Zhang Qiang , Shao Xiwen , Guo Liying
Published: August 2023

Soil salinization is a prevalent global environmental issue that significantly hampers crop growth and yield. However, there has been limited research on the impact of nitrogen fertilization and various management practices in alleviating saline-sodic stress in crops. In order to examine the impact of combined straw and nitrogen fertilizer application on the physiological and photosynthetic characteristics of rice in saline-sodic paddy fields, a three-year field experiment was conducted in Jilin Province, China. The experiment was conducted as a split-zone trial, where the main zone consisted of straw (S) and the secondary zone consisted of nitrogen fertilizer (N). Two levels of straw were 0 t ha−1 (B) and 7 t ha−1 (T). Four nitrogen treatments were applied: 0, 150, 250, and 350 kg ha−1, denoted as N0, N1, N2, and N3, respectively. The results show that the combination of straw and nitrogen fertilizer has been found to effectively reduce the Na+/K+ value, malondialdehyde content, and the relative electric leakage of rice leaves in saline-sodic soil. Furthermore, it increases leaf water potential, relative water content, and chlorophyll content, thereby promoting rice photosynthesis and improving rice yield. The rice yield exhibited the greatest positive effect when straw and nitrogen fertilizer were combined at a rate of 250 kg ha−1. The effectiveness of this combination improves over time. However, it is important to avoid excessive application of nitrogen fertilizer when using straw returning. This approach not only ensures stable rice yield in saline-sodic fields, but also has positive effects on the economic impact of fertilizer application and soil environment preservation.
Application of Fractal Theory to the Analysis of Failure Characteristics of Low-Velocity-Impact Concrete Slabs
Authors: Gu Song , Zhao Jiachen , Li Jinxing , Peng Feng , Kong Chao , Yang Liqiong
Published: August 2023

The fractal characteristics of low-velocity-impact concrete slabs were studied using fractal theory, and the fractal dimension value of cracks of each concrete specimen plate was calculated using box dimension as the basic principle and digital image analysis technology in conjunction with MATLAB software (R2021b) calculation functions. The energy dissipation of concrete slabs during low-velocity impact is calculated using the elastic sheet theory. The calculation results are realistic, and the energy conversion of concrete slabs during low-velocity impact is analyzed based on these results. The research findings indicate that concrete slab cracks exhibit good fractal characteristics during low-velocity impact, and their values can be utilized as a parameter to determine the extent of concrete slab failure. Moreover, the study found that the fractal dimension value of concrete slab cracks and the associated plastic deformation energy display good exponential function characteristics in the energy dissipation mechanism of low-velocity concrete slabs.
ACLP Activates Cancer-Associated Fibroblasts and Inhibits CD8+ T-Cell Infiltration in Oral Squamous Cell Carcinoma
Authors: Sekiguchi Shohei , Yorozu Akira , Okazaki Fumika , Niinuma Takeshi , Takasawa Akira , Yamamoto Eiichiro , Kitajima Hiroshi , Kubo Toshiyuki , Hatanaka Yui , Nishiyama Koyo , Ogi Kazuhiro , Dehari Hironari , Kondo Atsushi , Kurose Makoto , Obata Kazufumi , Kakiuchi Akito , Kai Masahiro , Hirohashi Yoshihiko , Torigoe Toshihiko , Kojima Takashi , Osanai Makoto , Takano Kenichi , Miyazaki Akihiro , Suzuki Hiromu
Published: August 2023

We previously showed that upregulation of adipocyte enhancer-binding protein 1 (AEBP1) in vascular endothelial cells promotes tumor angiogenesis. In the present study, we aimed to clarify the role of stromal AEBP1/ACLP expression in oral squamous cell carcinoma (OSCC). Immunohistochemical analysis showed that ACLP is abundantly expressed in cancer-associated fibroblasts (CAFs) in primary OSCC tissues and that upregulated expression of ACLP is associated with disease progression. Analysis using CAFs obtained from surgically resected OSCCs showed that the expression of AEBP1/ACLP in CAFs is upregulated by co-culture with OSCC cells or treatment with TGF-β1, suggesting cancer-cell-derived TGF-β1 induces AEBP1/ACLP in CAFs. Collagen gel contraction assays showed that ACLP contributes to the activation of CAFs. In addition, CAF-derived ACLP promotes migration, invasion, and in vivo tumor formation by OSCC cells. Notably, tumor stromal ACLP expression correlated positively with collagen expression and correlated inversely with CD8+ T cell infiltration into primary OSCC tumors. Boyden chamber assays suggested that ACLP in CAFs may attenuate CD8+ T cell migration. Our results suggest that stromal ACLP contributes to the development of OSCCs, and that ACLP is a potential therapeutic target.
Epithelioid Mesothelioma Patients with Very Long Survival Display Defects in DNA Repair
Authors: Ganzinelli Monica , Guffanti Federica , Ianza Anna , Sobhani Navid , Crovella Sergio , Zanconati Fabrizio , Bottin Cristina , Confalonieri Marco , Fumagalli Stefano , Guglielmi Alessandra , Generali Daniele , Damia Giovanna
Published: August 2023

Aim: DNA repair has an important role in malignant pleural mesothelioma (MPM) tumorigenesis and progression. Prognostic/predictive biomarkers for better management of MPM patients are needed. In the present manuscript, we analyzed the expression of more than 700 genes in a cohort of MPM patients to possibly find biomarkers correlated with survival. Methods: A total of 54 MPM patients, all with epithelioid histology, whose survival follow-up and formalin-fixed paraffin-embedded tumors were available, were included in the study. Gene expression profiles were evaluated using a Nanostring platform analyzing 760 genes involved in different cellular pathways. The percentages of proliferating tumor cells positive for RAD51 and BRCA1 foci were evaluated using an immunofluorescence assay, as a readout of homologous recombination repair status. Results: Patient median survival time was 16.9 months, and based on this value, they were classified as long and short survivors (LS/SS) with, respectively, an overall survival ≥ and <16.9 months as well as very long and very short survivors (VLS/VSS) with an overall survival ≥ than 33.8 and < than 8.45 months. A down-regulation in the DNA damage/repair expression score was observed in LS and VLS as compared to SS and VSS. These findings were validated by the lower number of both RAD51 and BRCA1-positive tumor cells in VLS as compared to VSS. Conclusions: The down-regulation of DNA repair signature in VLS was functionally validated by a lower % of RAD51 and BRCA1-positive tumor cells. If these data can be corroborated in a prospective trial, an easy, cost-effective test could be routinely used to better manage treatment in MPM patients.
Foxo3 Knockdown Mediates Decline of Myod1 and Myog Reducing Myoblast Conversion to Myotubes
Authors: Gellhaus Benjamin , Böker Kai O. , Gsaenger Marlene , Rodenwaldt Eyck , Hüser Marc A. , Schilling Arndt F. , Saul Dominik
Published: August 2023

Sarcopenia has a high prevalence among the aging population. Sarcopenia is of tremendous socioeconomic importance because it can lead to falls and hospitalization, subsequently increasing healthcare costs while limiting quality of life. In sarcopenic muscle fibers, the E3 ubiquitin ligase F-Box Protein 32 (Fbxo32) is expressed at substantially higher levels, driving ubiquitin-proteasomal muscle protein degradation. As one of the key regulators of muscular equilibrium, the transcription factor Forkhead Box O3 (FOXO3) can increase the expression of Fbxo32, making it a possible target for the regulation of this detrimental pathway. To test this hypothesis, murine C2C12 myoblasts were transduced with AAVs carrying a plasmid for four specific siRNAs against Foxo3. Successfully transduced myoblasts were selected via FACS cell sorting to establish single clone cell lines. Sorted myoblasts were further differentiated into myotubes and stained for myosin heavy chain (MHC) by immunofluorescence. The resulting area was calculated. Myotube contractions were induced by electrical stimulation and quantified. We found an increased Foxo3 expression in satellite cells in human skeletal muscle and an age-related increase in Foxo3 expression in older mice in silico. We established an in vitro AAV-mediated FOXO3 knockdown on protein level. Surprisingly, the myotubes with FOXO3 knockdown displayed a smaller myotube size and a lower number of nuclei per myotube compared to the control myotubes (AAV-transduced with a functionless control plasmid). During differentiation, a lower level of FOXO3 reduced the expression Fbxo32 within the first three days. Moreover, the expression of Myod1 and Myog via ATM and Tp53 was reduced. Functionally, the Foxo3 knockdown myotubes showed a higher contraction duration and time to peak. Early Foxo3 knockdown seems to terminate the initiation of differentiation due to lack of Myod1 expression, and mediates the inhibition of Myog. Subsequently, the myotube size is reduced and the excitability to electrical stimulation is altered.
Structural Analysis and Reactivity Insights of (E)-Bromo-4-((4-((1-(4-chlorophenyl)ethylidene)amino)-5-phenyl-4H-1,2,4-triazol-3-yl)thio)-5-((2-isopropylcyclohexyl)oxy) Furan-2(5H)-one: A Combined Approach Using Single-Crystal X-ray Diffraction, Hirshfeld Surface Analysis, and Conceptual Density Functional Theory
Authors: Bakheit Ahmed H. , Attwa Mohamed W. , Kadi Adnan A. , Alkahtani Hamad M.
Published: August 2023

This study presents a comprehensive exploration of the structure–reactivity relationship of (E)-3-bromo-4-((4-((1-(4-chlorophenyl)ethylidene)amino)-5-phenyl-4H-1,2,4-triazol-3-yl)thio)-5-((2-isopropylcyclohexyl)oxy)furan-2(5H)-one. The study embarked on an in-depth investigation into the solid-state crystal structure of this organic compound, employing computational Density Functional Theory (DFT) and related methodologies, which have not extensively been used in the examination of such compounds. A single-crystal X-ray diffraction (SCXRD) analysis was initially performed, supplemented by a Hirshfeld surfaces analysis. This latter approach was instrumental in visualizing and quantifying intermolecular interactions within the crystal structures, offering a detailed representation of the molecule’s shape and properties within its crystalline environment. The concept of energy framework calculations was utilized to understand the varied types of energies contributing to the supramolecular architecture of the molecules within the crystal. The Conceptual DFT (CDFT) was applied to predict global reactivity descriptors and local nucleophilic/electrophilic Parr functions, providing a deeper understanding of the compound’s chemical reactivity properties. The aromatic character and π–π stacking ability were also evaluated with the help of LOLIPOP and ring aromaticity measures. This comprehensive approach not only provides a detailed description of the structure and properties of the investigated compound but also offers valuable insights into the design and development of new materials involving 1,2,4-triazole systems.
Plant Functional Traits of Plants Species Colonizing Forest Gaps
Authors: Ravnjak Blanka , Bavcon Jože , Čarni Andraž
Published: August 2023

In this research, we analyzed functional plant traits and life forms of plant species growing on five forest gaps in the Dinaric fir–beech forest in Slovenia. The forest gaps were created as a result of natural disturbances. We selected 18 functional plant traits, whose values were obtained from the BiolFlor database. With the help of the JUICE program, we calculated the frequency of occurrence in individual forest gaps for each functional plant trait. Then, we calculated Spearman’s correlation coefficient at p < 0.05 between the occurrence of individual functional plant traits and each forest gap. Individual locations differed statistically in 87 categories of plant functional traits. The forest gap Nad Drago differed statistically from the other forest gaps in 16 functional plant traits. Forest gaps are mainly colonized by perennials and herbaceous perennials and chamaephytes. These are plant species that begin to flower in June and July, bloom for two or three months, and are pollinated by insects, mainly hover flies and wild bees. This colonizing plant species reproduce via seeds or spores and vegetatively. Furthermore, birds and forest mammals are the vectors of fruit and seed dispersal.
Post-Operative Modified All-Inside ACL Reconstruction Technique’s Clinical Outcomes and Isokinetic Strength Assessments
Authors: Genç Ahmet Serhat , Güzel Nizamettin , Yılmaz Ali Kerim , Ermiş Egemen , Pekesen Kurtça Mine , Agar Anıl , Ceritoğlu Kubilay Uğurcan , Yasul Yavuz , Eseoğlu İsmail , Kehribar Lokman
Published: August 2023

Background and Objective: Anterior cruciate ligament (ACL) injuries are very common among the athletic population. ACL reconstruction (ACLR) performed because of these injuries is one of the procedures performed by orthopedic surgeons using different grafting methods. This study aims to compare the data related to post-operative 6-month isokinetic strength values, strength-related asymmetry rates, time parameters, and joint angle in athletes who underwent ACLR with the Modified All-inside (4ST) technique, on both the healthy knee (HK) and the ACLR-applied sides. Materials and Methods: A total of 20 athletes from various sports on whom the 4ST ACLR technique had been applied by the same surgeon were evaluated retrospectively. Lysholm, Tegner, and International Knee Documentation Committee (IKDC) scores of the patients were obtained pre-operative and at 6 months post-operative. Isokinetic knee extension (Ex) and flexion (Flx) strengths on the HK and ACLR sides of the patients were evaluated with a series of four different angular velocities (60, 180, 240, and 300°/s). In addition to peak torque (PT) and hamstring/quadriceps ratio (H/Q) parameters, the findings were also evaluated with additional parameters such as joint angle at peak torque (JAPT), time to peak torque (TPT), reciprocal delay (RD), and endurance ratio (ER). Results: There was a significant improvement in the mean Lysholm, Tegner, and IKDC scores after surgery compared with pre-operative levels (p < 0.05). As for PT values, there were significant differences in favor of the HK in the 60, 180, and 300°/s Ex phases (p < 0.05). In terms of the H/Q and (hamstring/hamstring)/(quadriceps/quadriceps) (HH/QQ) ratios, there were significant differences at 300°/s (p < 0.05). In terms of JAPT, there were significant differences in the 300°/s Ex and 180°/s Flx phases (p < 0.05). In terms of TPT, there were significant differences in the 300°/s Ex phase (p < 0.05). In terms of RD and ER, no significant difference was observed between the HK and ACLR sides at any angular velocity. Conclusions: Although differences were observed in PT values, particularly in the Ex phase, this did not cause a significant change in H/Q ratios. Similar results were observed for additional parameters such as JAPT, TPT, RD, and ER. The results show that this ACLR technique can be used in athletes in view of strength gain and a return to sports.
Endoscopy Ultrasound-Guided Biliary Drainage Using Lumen Apposing Metal Stent in Malignant Biliary Obstruction
Authors: Fugazza Alessandro , Andreozzi Marta , De Marco Alessandro , Da Rio Leonardo , Colombo Matteo , Spadaccini Marco , Carrara Silvia , Giacchetto Marco , Sharma Mrigya , Craviotto Vincenzo , Busacca Anita , Ferrari Chiara , Repici Alessandro
Published: August 2023

This narrative review provides an overview of the application of endoscopic ultrasound-guided biliary drainage (EUS-BD), including EUS-guided gallbladder drainage (EUS-GBD), for the treatment of malignant biliary obstruction. EUS-BD has demonstrated excellent technical and clinical success rates, with lower rates of adverse events when compared with percutaneous trans-hepatic biliary drainage (PTBD). EUS-BD is currently the preferred alternative technique for biliary drainage (BD) in patients with distal malignant biliary obstruction (DMBO) after failed endoscopic retrograde cholangiopancreatography (ERCP). Particularly, this review will focus on EUS-BD performed with the use of lumen apposing metal stent (LAMS). The introduction of these innovative devices, followed by the advent of electrocautery-enhanced LAMS (EC-LAMS), gave the procedure a great technical implementation and a widespread application.
Post-Operative Greater Tuberosity Resorption or Malreduction Is Associated with Poor Prognostic Outcomes in Patients with Proximal Humeral Fractures Treated Operatively—A Single-Center Retrospective Cohort Study
Authors: Lu Kuan-Yu , Tai Ting-Han , Liu Yu-Hsin , Chiang Chang-Jung , Loh El-Wui , Wong Chin-Chean , Wu Jeffrey J.
Published: August 2023

(1) Background: Proximal humerus fractures can be a debilitating condition if not properly treated. These fracture patterns are varied and differ in every patient. Functional outcomes may be determined by the integrity of the shoulder girdle involving the rotator cuff insertion. The post-operative resorption or malreduction of the greater tuberosity (GT) is an important factor contributing to the poor functional outcome of a patient. Thus, we intend to evaluate the cause-and-effect relationship between GT complications and clinical prognosis and outcomes. (2) Methods: A single-center retrospective comparative study was performed to evaluate the functional outcomes of patients undergoing operative fixation for this injury. A total of 387 consecutive cases treated operatively from 2019–2021 were included for analysis. (3) Results: 94 cases fulfilled our criteria for analysis. A matched-group comparison of 19 patients each was performed to compare demographics, post-operative fracture characteristics and clinical outcomes. (4) Conclusions: The resorption or malreduction of the GT contributes greatly to the prognostic outcome in patients treated with open reduction and internal fixation (ORIF) surgery. In our demographic study, obesity is another contributing factor affecting the parameters of post-operative reduction in proximal humerus fractures. Appropriate surgical planning and post-operative multidisciplinary care must be taken into consideration to attain a satisfactory prognostic outcome.
Three-Year Follow-Up Detecting Choroidal Neovascularization with Swept Source Optical Coherence Tomography Angiography (SS-OCTA) after Successful Half-Fluence Photodynamic Therapy for Chronic Central Serous Chorioretinopathy
Authors: Esteban-Floría Olivia , Pérez-Rivasés Guillermo , Honrubia-Grijalbo Ana , Bartolomé-Sesé Isabel , Díaz-Barreda María Dolores , Boned-Murillo Ana , Cisneros-Arias Pablo , Mateo-Gabás Javier , Ascaso-Puyelo Francisco-Javier
Published: August 2023

Purpose: To assess the clinical course, structural changes, and choroidal neovascularization detection by SS-OCTA in long-standing and resolved patients with chronic central serous chorioretinopathy (cCSC) after successful half-fluence photodynamic therapy (hf-PDT) treatment. Methods: Twenty-four eyes presenting with cCSC were examined with SS-OCTA and were classified as choroidal neovascular (CNV) or non-choroidal neovascular (non-CNV) cCSC depending on the vascular pattern detected by SS-OCTA after one, two, and three years after hf-PDT. Two groups were compared based on the following clinical findings: demographic characteristics, time of clinical signs, best corrected visual acuity (BCVA), central retinal thickness (CRT), central choroidal thickness (CFT), subretinal fluid (SRF), flat, irregular pigment epithelial detachment (FIPED), and features of fluorescein angiography (FA) and vascular pattern by SS-OCTA. Results: All patients showed resolved cCSC during follow-up after hf-PDT. A total of 5 of 24 (20.8%) eyes showed a neovascular pattern by SS-OCTA. No differences between BCVA, CRT, SRF, FIPED, or FA features were found between both groups (p > 0.05). However, CFT and older age were associated with a neovascular pattern by SS-OCTA (p < 0.05) in follow-up. No signs of neovascular activity were detected by SS-OCT during follow-up in CNV cCSC patients, and no antiVEGF treatment was required for three-year follow-ups. Conclusions: Despite patients with cCSC showing a favorable clinical response after hf-PDT, lower foveal thickness and older age were associated with CNV patterns by SS-OCTA during follow-up.
Precision Medicine in Inflammatory Bowel Disease
Authors: Annese Vito , Annese Monica
Published: August 2023

Ulcerative colitis and Crohn’s disease are traditionally defined as the two main subtypes of inflammatory bowel disease. However, a more recent view considers IBD as a spectrum of heterogeneous phenotypes with consistent differences in clinical presentation and behaviors, likely explained by differences in underlying pathogenetic mechanisms. The etiology is still elusive, and the suggested pathogenesis is a complex interplay among genetic predisposition and abnormal immune response at the mucosal intestinal level, activated by only partially identified environmental triggers leading to altered intestinal permeability and impaired handling of gut microbiota. The undeniable continuous progress of medical therapy with more frequent shifts from traditional to more advanced modalities also underlines the actual unmet needs. We are using medications with completely different mechanisms of action, with a lack of predictive factors of outcomes and response and still an unsatisfactory rate of success. In addition, we are missing still valuable and accurate markers to predict disease progression and severity in order to avoid under- or over-treatment. In such a complex scenario, it is undoubtful that the application of artificial intelligence and machine learning algorithms may improve the management and pave the way for precision and eventually personalized medicine in these patients; however, there are still several challenges that will be the focus of this review.
Unveiling Asthma’s Complex Tapestry: Insights from Diverse Perspectives
Authors: Anand Mahesh Padukudru
Published: August 2023

Asthma, a chronic respiratory disorder affecting millions worldwide, exhibits considerable heterogeneity in its clinical presentation, severity, and response to therapy [...]
REERP: A Region-Based Energy-Efficient Routing Protocol for IoT Wireless Sensor Networks
Authors: Dogra Roopali , Rani Shalli , Gianini Gabriele
Published: August 2023

An essential component of the Internet of Things (IoT) is wireless sensor networks (WSNs). Since individual sensor nodes are strongly power-constrained, several techniques are adopted to save power. By grouping nodes into clusters—thus reducing the transmission distance between sensor nodes and the base station (BS)—a clustering protocol can ensure energy preservation and increase the lifetime of the network. However, current clustering techniques have problems with the clustering structure that negatively impact their performance. Whenever routing protocols were implemented for a longer period of time, it was observed that they had a higher rate of energy consumption, a shorter period of stability, and fewer data transfers to the BS. In this paper, an improved region-based routing protocol (REERP) is developed for wireless sensor networks in the IoT is developed. It is based on (i) the addition of new nodes to the already formed clusters, (ii) the selection of the new head node based on the amount of residual energy, (iii) the setup of the multi-hop communication in all the regions of network, and (iv) the utilization of the energy hole reduction method. All of these tactics increase the useful life of the network. Performance has been evaluated against (1) a stable election protocol, (2) a gateway energy-aware routing protocol, and (3) a heterogeneous gateway energy-aware routing protocol, and using the metrics lifetime, energy consumption, number of dead nodes, and number of packets sent to the base station vs. number of packets acquired by the base station. The results of the proposed routing protocol have been found to outperform the state-of-the-art approaches considered.
Measures and Prescriptions to Reduce Stray Current in the Design of New Track Corridors
Authors: Vranešić Katarina , Bhagat Sahil , Mariscotti Andrea , Vail Robert
Published: August 2023

Stray currents can cause very rapid degradation and material loss at the points where the current leaves the metal and enters the electrolyte. Nowadays, many resources are invested in the protection of jeopardized structures, such as buried pipelines, from stray current corrosion. This paper describes the measures that need to be considered in the design and construction of track structures to ensure high rail-to-ground resistance and consequently reduce stray currents. The main conclusions from existing guidelines and standards for reducing and controlling stray currents that are applied by various track operators are presented in the paper. Rail-to-ground resistance in different types of tracks structures and rail fastening systems is analyzed, and the optimal type of the track and type of the fastening system is defined. The grounding schemes used on the tracks and their influence on stray current values are described, as well as the influence of traction power stations (TPS) and rail cross bonding on stray current. Since it is not necessary to apply all the measures described to the same track structure, the paper gives recommendations on which measures to apply when building tracks with continuously fastened rails and which to apply when building tracks with discretely supported and fastened rails.
A Review of Water-Energy-Food Nexus Development in a Just Energy Transition
Authors: Li Yan , Zhang Ruilian
Published: August 2023

The water-energy-food (WEF) nexus has emerged as a crucial framework for addressing the interdependencies and trade-offs between these vital resources. In the context of a just energy transition, where the pursuit of sustainable and equitable energy systems is paramount, understanding the WEF nexus becomes even more critical. We explore the evolving concept of the WEF nexus and its relevance to achieving a sustainable energy transition that considers social equity, environmental sustainability, and economic development. This paper highlights key challenges and opportunities in implementing a just energy transition within the context of the WEF nexus, with a focus on promoting social inclusion, ensuring water and food security, and optimizing energy production and consumption. Additionally, we discuss the importance of integrated policymaking, cross-sectoral collaboration, and innovative technologies in addressing the complex interactions between water, energy, and food systems.
Actions to Handle Increasing Distributed Generation and Rising Voltage during Low-Demand Periods: Tap Staggering Practices and Field Tests in the Italian Transmission Network
Authors: Quaia Stefano , Mauri Alessandro , Marchesin Alessio , Manià Paolo
Published: August 2023

This paper deals with the voltage regulation strategies implemented in the Italian transmission network to face the increasing problem of high voltages during time periods characterized by low demand. After an introduction in which this very actual problem is discussed, the focus is on tap staggering practices. Although tap staggering is not a new idea, it is gaining practical importance only in the very last few years as a means of enhancing the inductive power drawn from the grid and, therefore, limiting the voltage rise. Accordingly, tap staggering contributes to the mitigation of the problems caused by the increasing penetration of renewable energy sources and thus can allow an increase in the share of renewable energy sources. The paper presents the different tap staggering practices that are being defined by the Italian transmission system operator and reports some tap staggering tests recently performed on large autotransformers as well as phase-shifter transformers.
Assessing the Potential of Integrated Shading Devices to Mitigate Overheating Risk in University Buildings in Severe Cold Regions of China: A Case Study in Harbin
Authors: Qin Siqi , Liu Ying , Yu Ge , Li Rulin
Published: August 2023

Creating a comfortable indoor environment in education buildings is an important design objective. Climate change has resulted in rising summer indoor temperatures in the severe cold regions of China, and evidence of summer overheating risk in these regions has not yet been fully investigated. This study presents evidence of overheating in a university building in a severe cold region of China, discusses the potential of integrated shading devices for mitigating overheating, and proposes design ideas for the application of shading devices. Temperature monitoring and simulation were performed in a university building with natural ventilation located in Harbin, and various configurations of integrated shading devices were simulated using IES Virtual Environment software. The results demonstrate that 69% of classrooms were overheated; furthermore, south-facing classrooms could be overheated for up to 152 h during summer occupancy hours. This study finds that integrated shading devices reduce overheating hours by up to 59.2%. The design of appropriate parameters for shading devices can effectively improve indoor thermal comfort while maintaining daylight levels and controlling the increase in energy consumption. The methodology and results presented in this study offer a reference point and practical guidance for mitigating regional overheating, aiming to promote the improvement of regional standards and optimisation of thermal environments in the severe cold regions of China.