bluuebunny/crossref_metadata_2025 huggingface dataset:
Modelling Laboratory Fischer-Tropsch Synthesis Using Cobalt Catalysts
Authors: Díaz-Trujillo Luis A. , Toledo-Chávez Gilberto , Jiménez-García Gladys , Hernández-Escoto Héctor , Maya-Yescas Rafael
Published: April 2018

The main goal of this paper is to critically review current microkinetics available for Fischer-Tropsch synthesis (FTS) modelling, in order to propose the best way to follow this set of complex reactions; therefore a microkinetic model was developed for FTS, accomplishing surface chemistry, heterogeneous kinetics, and single-event previous development for Co-based catalysts. Model starts simulating CO activation on catalyst surface, and then formation of methane, ethane and subsequent chain growth. Reaction rates were derived following the formalism of Langmuir-Hinshelwood-Hougen-Watson (LHHW); surface steps were proposed in consequence of feasibility. Chain growth was modelled by single-event steps, taking into account geometrical conformation explicitly. Number of growth steps of 1-olefins was rectified as requiring one more active site than mechanisms proposed previously; it was found that formation of these olefins exhibits a fast drop in chain growth; this phenomenon is explained in terms of probable geometrical conformations that lead to the number of single events. Experimental results in literature about isothermal synthesis of hydrocarbons in the gasoline range were simulated in a fixed-bed laboratory reactor; thermodynamic consistency was derived from chemical equilibrium over all reactions occurring during FTS. Equilibrium constants were evaluated as function of Gibbs free energy, and partial pressures of reactants and products.
Demand for Law in the African Private Sector
Authors: Finnegan David L.
Published: April 2018

This article develops a model for explaining private sector response to law reforms in developing countries. The dependent variable in this model is the demand for law – the extent to which business owners and firm managers engage the formal legal system and attempt to mobilize the law to their advantage when making market decisions. Demand for law is a crucial micro-connection in the causal chain linking law and law reform to market expansion and economic development. The theoretical framework developed here focuses on the interplay between informal and formal social institutions in influencing market decisions made by private sector actors. The article tests hypotheses generated by this framework by analyzing survey data collected from business firms in Dar es Salaam, Tanzania. The results support the hypothesis that demand for law is shaped by informal market institutions, particularly the informal business networks developed and maintained by firms. The analysis further suggests that these “demand-side” variables matter more than “supply-side” variables identified in the social science literature. These findings have important implications for law reform initiatives in developing countries and suggest avenues for further social science research on the relationship between law and economic development.
Mechanisms of Formation of Human Culture in Education
Authors: Baboshina Helen B.
Published: April 2018

The relevance of the research problem lies in the necessity of an axiological approach to the formation of the personality in education and the task of strengthening the ideal image of the function. The aim of this article is studying and understanding the culture of personality formation mechanisms in relation to future specialists. The leading method of research was the theoretical analysis of philosophical and cultural approaches to the cultural formation of the personality and to the content of human culture. Content analysis was based on the philosophical and cultural concepts of V. S. Bibler, M. Buber, J. G. Herder, I. Kant, L. N. Kogan, D. S. Likhachev, A. Schweitzer, M. Scheler, and others. The experiment method was the experimental realization of formation stages of the future specialist as a person of culture, which allowed revealing the positive role of cultural mechanisms in this process. The result is the stages of human culture formation as well as mechanisms for their implementation. The article may be useful for specialists of the educational sphere, social philosophers, and culturologists.
Transplantation is Not Enough..., or on the Concept of Xenotransplantation
Authors: Guzik-Makaruk Ewa M. , Perkowska Marta M.
Published: April 2018

The paper presents the emergence of the concept of xenotransplantation which is a relatively new issue in the literature on the subject. It is due to the fact that transplants of animal organs are currently in the experimental phase. The main current problem of transplantology is the shortage of organs; hence, the search for new solutions has become an everyday challenge. If a way for the human body to tolerate animal organs could be found, transplant medicine and humanity would be in a completely different place. The authors introduce the concepts of transplantation and xenotransplantation and their origins, then they raise ethical issues related to this type of organ transplantation. Finally, the authors conclude that xenotransplantations have a chance to be “incorporated” (back again) in the “transplants” category when the experimental phase of xenotransplantation shifts to the implementation stage. Time will tell whether it will be possible.
Autism Between the PhD Student and the Promotor. A Case Study
Authors: Perkowski Maciej , Oksztulski Maciej , Kaczyńska Izabela
Published: April 2018

Contemporary societies struggle with the problem of education being inadequate to the reality. The crisis (and, consequently, the deficit) of authorities is present in all levels of education. It seems that the classical vertical mechanism “student-master” should experience a renaissance. Instead of theoretical argumentation, it is worth learning about the case of a particular relationship – between a doctoral student who is a non-speaking autistic person and the promotor who tries to oppose it constructively. Both lawyers (as the cooperation is currently taking place in doctoral law studies) apart from the preparation of the dissertation, are experimenting methodologically, entering the “student-master” relationship with mutual benefit. They are subject to ongoing verification in this area – both domestic and foreign.
Multilingualism in the EU and Consistency of Private Enforcement of Competition Law: Two Examples from CEE Countries
Authors: Piszcz Anna
Published: April 2018

This paper attempts to address the question of how multilingualism in the EU might affect the consistency of private enforcement of competition law. In the literature, there have been concerns raised about the consistency of public enforcement of competition law, so in this paper attention has shifted to concerns about consistency of private enforcement. For the purposes of this paper, a distinction is drawn between rule-making and the application of competition law. The latter falls outside the scope of this paper. The article starts by going straight into aspects of public versus private enforcement of EU competition law and consistency of private enforcement of competition law. Next, by looking at examples of national rules implementing the EU Damages Directive, the author is going to discern what challenges for consistency of private enforcement of competition law are associated with the multilingualism in the EU.
Attempts for Common Understanding of the Concept of Worker as a Consequence of Globalisation?
Authors: Ryszka Joanna
Published: April 2018

Globalisation may concern many different issues, among others, the increase in migration that creates opportunities for all. There should be no doubt that globalisation can bring both positive and negative effects to workers. It can be seen as new opportunities for people, because they can travel, work, learn and live in different countries. Simultaneously however it can be perceived as synonymous to job losses, social injustice, or low environmental, health, and privacy standards. As a result of globalisation, the world is becoming more and more complex and the economic importance of state borders is reduced. It should therefore not raise doubts, that global problems require the capacity to agree on coordinated global responses and mechanisms on the basis of international cooperation. Among the basic international organisations which provide solutions for workers who have decided to look for a job in another country, one can generally mention the International Labour Organisation (ILO), the Council of Europe, and the European Union (EU). There is quite a large number of legal acts created in the framework of those organisations, and so we should ask a question if in such a situation we should also try to understand some legal concepts; in our case concepts connected with taking up employment, in a similar way. Even if the answer is positive, another question comes to mind – is it possible to have such definitions in a global world? It is thus not enough to provide legal regulations concerning worker’s rights and obligations if we do not know who exactly should be treated like a worker. The following article will try to answer those questions and simultaneously try to show that globalisation may affect the way certain terms should be understood.
Sound Frequency Representation in the Auditory Cortex of the Common Marmoset Visualized Using Optical Intrinsic Signal Imaging
Authors: Tani Toshiki , Abe Hiroshi , Hayami Taku , Banno Taku , Miyakawa Naohisa , Kitamura Naohito , Mashiko Hiromi , Ichinohe Noritaka , Suzuki Wataru
Published: April 2018

Natural sound is composed of various frequencies. Although the core region of the primate auditory cortex has functionally defined sound frequency preference maps, how the map is organized in the auditory areas of the belt and parabelt regions is not well known. In this study, we investigated the functional organizations of the core, belt, and parabelt regions encompassed by the lateral sulcus and the superior temporal sulcus in the common marmoset (Callithrix jacchus). Using optical intrinsic signal imaging, we obtained evoked responses to band-pass noise stimuli in a range of sound frequencies (0.5–16 kHz) in anesthetized adult animals and visualized the preferred sound frequency map on the cortical surface. We characterized the functionally defined organization using histologically defined brain areas in the same animals. We found tonotopic representation of a set of sound frequencies (low to high) within the primary (A1), rostral (R), and rostrotemporal (RT) areas of the core region. In the belt region, the tonotopic representation existed only in the mediolateral (ML) area. This representation was symmetric with that found in A1 along the border between areas A1 and ML. The functional structure was not very clear in the anterolateral (AL) area. Low frequencies were mainly preferred in the rostrotemplatal (RTL) area, while high frequencies were preferred in the caudolateral (CL) area. There was a portion of the parabelt region that strongly responded to higher sound frequencies (>5.8 kHz) along the border between the rostral parabelt (RPB) and caudal parabelt (CPB) regions.
Dynamic Encoding of Incentive Salience in the Ventral Pallidum: Dependence on the Form of the Reward Cue
Authors: Ahrens Allison M. , Ferguson Lindsay M. , Robinson Terry E. , Aldridge J. Wayne
Published: April 2018

Some rats are especially prone to attribute incentive salience to a cue (conditioned stimulus, CS) paired with food reward (sign-trackers, STs), but the extent they do so varies as a function of the form of the CS. Other rats respond primarily to the predictive value of a cue (goal-trackers, GTs), regardless of its form. Sign-tracking is associated with greater cue-induced activation of mesolimbic structures than goal-tracking; however, it is unclear how the form of the CS itself influences activity in neural systems involved in incentive salience attribution. Thus, our goal was to determine how different cue modalities affect neural activity in the ventral pallidum (VP), which is known to encode incentive salience attribution, as rats performed a two-CS Pavlovian conditioned approach task in which both a lever-CS and a tone-CS predicted identical food reward. The lever-CS elicited sign-tracking in some rats (STs) and goal-tracking in others (GTs), whereas the tone-CS elicited only goal-tracking in all rats. The lever-CS elicited robust changes in neural activity (sustained tonic increases or decreases in firing) throughout the VP in STs, relative to GTs. These changes were not seen when STs were exposed to the tone-CS, and in GTs there were no differences in firing between the lever-CS and tone-CS. We conclude that neural activity throughout the VP encodes incentive signals and is especially responsive when a cue is of a form that promotes the attribution of incentive salience to it, especially in predisposed individuals.
Squamous Cell Carcinoma of the Lower Lip Arising in Discoid Lupus Lesion - A Case Report
Authors: Sbai Achraf Amine , Eabdenbitsen Adil , Elayoubi Fahd , Ghailan Mohamed Rachid
Published: April 2018

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Evaluation of the therapeutic interventions effects on body growth pattern of infants with congenital hypothyroidism
Authors: Etemad Koorosh , Khazaei Zaher , Pordanjani Sajjad Rahimi , Shahsavand Masoumeh , Ajam Fatemeh , Riahi Seyed-Mohammad , Zare Zahra , Iranpour Sohrab , Darvishi Isan , Sohrabivafa Malihe
Published: April 2018

Background: The congenital hypothyroidism (CH) is one of the most common congenital endocrine disorders as well as the most preventable mental retardation cause and body growth disorder. Several factors such as therapeutic interventions might affect the infants’ growth status. The current study was aimed to evaluate the therapeutic interventions’ effect on growth pattern of infants with congenital hypothyroidism in Yazd.
Methods: This retrospective cohort study was performed on all neonates with CH, who were born during years 2006 and 2008. The effectiveness of therapeutic interventions indices including the age of treatment onset, the administered dosage of Levothyroxine and normalization time of T4 and TSH level on growth pattern of children with CH by measuring height, weight and head circumference during the first five years was evaluated.
Results: The results of the current study showed a significant increase in height, weight, and head circumference in infants from early infancy to 60 months of age as well as an ascending trend of the aforementioned variables(P<0.05).
Conclusion: The significant effect of therapeutic interventions such as the age of treatment onset less than 30 days, normalization time of TSH during 30 days after diagnosing and normalization age of T4 less than 14 days was reported on height and weight growths of patients with congenital hypothyroidism. Also, the trend of head circumference growth would be increased in patients equally.
Mortality After Pediatric Arterial Ischemic Stroke
Authors: Beslow Lauren A. , Dowling Michael M. , Hassanein Sahar M.A. , Lynch John K. , Zafeiriou Dimitrios , Sun Lisa R. , Kopyta Ilona , Titomanlio Luigi , Kolk Anneli , Chan Anthony , Biller Jose , Grabowski Eric F. , Abdalla Abdalla A. , Mackay Mark T. , deVeber Gabrielle ,   on behalf of the International Pediatric Stroke Study Investigators
Published: April 2018

OBJECTIVES:

Cerebrovascular disease is among the top 10 causes of death in US children, but risk factors for mortality are poorly understood. Within an international registry, we identify predictors of in-hospital mortality after pediatric arterial ischemic stroke (AIS).

METHODS:

Neonates (0–28 days) and children (29 days–<19 years) with AIS were enrolled from January 2003 to July 2014 in a multinational stroke registry. Death during hospitalization and cause of death were ascertained from medical records. Logistic regression was used to analyze associations between risk factors and in-hospital mortality.

RESULTS:

Fourteen of 915 neonates (1.5%) and 70 of 2273 children (3.1%) died during hospitalization. Of 48 cases with reported causes of death, 31 (64.6%) were stroke-related, with remaining deaths attributed to medical disease. In multivariable analysis, congenital heart disease (odds ratio [OR]: 3.88; 95% confidence interval [CI]: 1.23–12.29; P = .021), posterior plus anterior circulation stroke (OR: 5.36; 95% CI: 1.70–16.85; P = .004), and stroke presentation without seizures (OR: 3.95; 95% CI: 1.26–12.37; P = .019) were associated with in-hospital mortality for neonates. Hispanic ethnicity (OR: 3.12; 95% CI: 1.56–6.24; P = .001), congenital heart disease (OR: 3.14; 95% CI: 1.75–5.61; P < .001), and posterior plus anterior circulation stroke (OR: 2.71; 95% CI: 1.40–5.25; P = .003) were associated with in-hospital mortality for children.

CONCLUSIONS:

In-hospital mortality occurred in 2.6% of pediatric AIS cases. Most deaths were attributable to stroke. Risk factors for in-hospital mortality included congenital heart disease and posterior plus anterior circulation stroke. Presentation without seizures and Hispanic ethnicity were also associated with mortality for neonates and children, respectively. Awareness and study of risk factors for mortality represent opportunities to increase survival.
La enseñanza de la escritura en FLE: los contextos divergentes según los profesores
Authors: Guerra Joaquim
Published: April 2018

Este artículo busca comprender las razones que llevan a los profesores de Francés Lengua Extranjera (FLE) de la enseñanza básica y secundaria a relegar a un segundo plano el desarrollo de la competencia de producción escrita, a través del estudio de las reflexiones de profesores acerca de sus prácticas de enseñanza de la escritura. A partir de un abordaje comprensivo de análisis de datos recogidos a través de entrevistas, se encontró que las principales dificultades en la enseñanza de la escritura registradas por los sujetos remiten a contextos extrínsecos, no dependientes de la acción de los docentes implicados. De este modo, discutiremos los obstáculos referidos por los docentes en cuanto a la implementación de prácticas de enseñanza de la escritura, a las dificultades observadas en los alumnos y, finalmente, a las estrategias implementadas para resolver las dificultades referidas.
Uso del diario interactivo para fomentar la escritura en ELE: Volviendo al papel y al lápiz
Authors: Villalobos José
Published: April 2018

La escritura es considerada por algunos investigadores como una actividad mental algo compleja y, por ello, un poco difícil de desarrollar, sobre todo en una lengua extranjera. En el contexto del aula de clases de una lengua extranjera, en ocasiones, los estudiantes inscritos en cursos de español necesitan producir textos escritos y esta actividad les causa cierto grado de angustias y frustraciones. Un grupo de estudiantes de español inscritos en el curso de Español Intermedio II no fue la excepción. La presente investigación es un estudio de casos de corte cualitativo que realicé con estudiantes de español como lengua extranjera, a nivel de pregrado. En dicho curso, implementé el diario interactivo como estrategia pedagógica. El estudio tuvo como propósitos principales investigar: 1) hasta qué punto uno de mis estudiantes estaría motivado a producir textos escritos utilizando el diario interactivo y 2) la calidad y cantidad de sus producciones escritas. 16 estudiantes participaron en este estudio, pero solo reporto los resultados de uno de ellos, ya que es un estudio de casos. El estudio incluyó observaciones, notas de campo y dos entrevistas semi-estructuradas con el propósito de triangular toda la información. Los resultados de esta investigación demostraron que los estudiantes mejoraron la organización del pensamiento y de sus ideas mientras escribían en sus diarios, desarrollaron una actitud positiva hacia la escritura en español y utilizaron el diario interactivo para generar temas y así producir composiciones más extensas en español para expresar sus pensamientos y sentimientos en relación a temas que se discutieron en clase. Al final del trabajo, presento algunas recomendaciones de tipo pedagógico para el uso del diario interactivo en cursos de español como lengua extranjera.
Polifonia retrofuturista: a (re)criação de vozes literárias na série Brasiliana Steampunk
Authors: Tavares Enéias Farias
Published: April 2018

Neste ensaio, apresentarei o processo de criação da série Brasiliana Steampunk através de três fenômenos culturais: a apropriação de obras em domínio público, a justaposição de diferentes personagens em um único universo e a utilização da estética steampunk. Num segundo momento, discutirei como procedi na recriação dos heróis clássicos da literatura brasileira. Por fim, mostrarei como esse trabalho de adaptação de vozes literárias preexistentes me levou ao desenvolvimento de três personagens inéditos do romance A lição de anatomia do temível dr. Louison: o investigador Pedro Britto Cândido, a escritora feminista Beatriz de Almeida & Souza e o médico e esteta Antoine Louison.
Just a note
Authors: De Campos Beatriz Badim
Published: April 2018

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Olhar, memória, imaginário em Sebastião Salgado
Authors: Maia Paulo
Published: April 2018

Este artigo busca compreender a transformação do olhar, da memória e do imaginário na fotografia de Sebastião Salgado em sua fotografia documental dos anos 1970 até 2000. Sentimento de travessia é uma formulação para o olhar, as memórias e o imaginário do fotógrafo sobre as novas morfologias no mundo do trabalho no período pós-industrial. Outras Américas (1986), seu primeiro ensaio publicado em livro, apresenta o problema do êxodo rural. Seu olhar sobre o tema, no entanto, é ainda incerto. Muito distante da idealização do primeiro ensaio, o livro Êxodos é uma síntese das transformações operadas no planeta a partir da nova morfologia do trabalho no final do século XX. Se em Outras Américas a travessia é contemplativa, em Êxodos a travessia é a força que orienta a transformação numa realidade marcada pela combinação de concentração de riquezas e inanição da classe trabalhadora.
Three-dimensional structure of the complex between calmodulin mutant lacking the c-terminal five residues and the calmodulin-binding peptide derived from skeletal muscle myosin light-chain kinase
Authors: Dung Vu Van , Umetsu Yoshitaka , Ohki Shinya
Published: April 2018

In our previous study, functional ability and conformational stability had been examined for C-terminal deletion mutants of a 148-residue Ca2+-binding protein, chicken calmodulin (CaM). In that study, we had reported that a mutant named CCMΔ5, missing five residues at the C-terminus, activates CaM-target as much as full-length CaM does. This finding is intriguing because CCMΔ5 lacks the key residues, Met144 and Met145, for the target activation. To uncover why CCMΔ5 displays proper function, here we report the three-dimensional structure of CCMΔ5 bound to the peptide derived from skeletal muscle myosin light-chain kinase (skMLCK). The structure determination was achieved using multidimensional nuclear magnetic resonance (NMR) spectroscopy. The complex structure of CCM∆5-skMLCK was compared to that of wild CaM-skMLCK. The results showed that the orientation of helix-1 and helix-5 in CCM∆5 differs from those in wild CaM. Moreover, distinctive hydrophobic interaction manner was found in the binding between CCM∆5 and peptide; Phe141, Ala128, Met109, Leu105 and Phe92 of CCM∆5 contribute to the interaction with Trp4 of the skMLCK peptide.   Abbreviations: CaM, calmodulin; CCMΔX, a deletion mutant of CaM that lacks X C-terminal residues; NMR, Nuclear magnetic resonance; PDB, Protein date bank; skMLCK, skeletal muscle myosin light-chain kinase; TOF-MS, Time-of-flight mass spectrometry; RMSD, root mean square deviation; SDS-PAGE, Sodium dodecyl sulfate polyacrylamide gel electrophoresis Citation: Vu Van Dung, Umetsu Y., Ohki S., 2017. Three-dimensional structure of the complex between calmodulin mutant lacking the c-terminal five residues and the calmodulin-binding peptide derived from skeletal muscle myosin light-chain kinase. Tap chi Sinh hoc, 39(3): 309-319. DOI: 10.15625/0866-7160/v39n3.10111. *Corresponding author: vandungbio46@yahoo.com Received 19 June 2017, accepted 20 August 2017
Study on biocharacteristics of Symbiodinium sp. isolated from soft coral in coastal water of Quang Nam, south central Vietnam
Authors: Hong Dang Diem , Nhat Pham Van , Huong Quynh Hoang Thi , Tam Luu Thi , Hoai Thu Ngo Thi , Ha Nguyen Cam , Lan Anh Hoang Thi , Nam Nguyen Hoai , Minh Hang Nguyen Thi , Minh Chau Van
Published: April 2018

Phototrophic dinoflagellates of the genus Symbiodinium (= zooxanthellae) are well known as symbionts of corals. They play an important role in maintaining the healthy as well as establishment of new reefs. In addition, products such as toxins or pigments from these symbiotic microalgae are potentially important for applications in the biomedical sciences, especially in new drug development. In this study, we isolated successfully a symbiotic microalga from soft coral Sinularia sp. which was collected in Cu Lao Cham coastal waters of Quang Nam province. Based on morphological characteristics and 18S rRNA gene sequences, isolated SHM8 strain was identified as Symbiodinium goreaui. This symbiont was cultured in different media such as ASP-A8, F2, ES, IMK, K, and L1. L1 culture medium supplemented with taurine concentration of 4.88 mM led to the cell density of SHM8 strain increased in 50% compared to control on day 2 in cultivation; amino acid mixture enhanced cell density by 31.7% on day 5 in cultivation. Supplementation of carbon source (bicarbonate salt of 16.8 g/L) and nitrogen source (nitrate salt of 0.055 g/L) enhanced the cell density by 40% and 27%, respectively on day 6 in culture. Obtained results in this study proved that this microalga can live independently without host. Successful isolation and cultivation of Symbiodinium microalga are one of the necessary conditions for promising researches on the natural bioactive substances from this microalgal biomass in the future in Vietnam.   Citation: Dang Diem Hong, Pham Van Nhat, Hoang Thi Huong Quynh, Luu Thi Tam, Ngo Thi Hoai Thu, Nguyen Cam Ha, Hoang Thi Lan Anh, Nguyen Hoai Nam, Nguyen Thi Minh Hang, Chau Van Minh, 2017. Study on biocharacteristics of Symbiodinium sp. isolated from soft coral in coastal water of Quang Nam, south central Vietnam. Tap chi Sinh hoc, 39(3): 367-375. DOI: 10.15625/0866-7160/v39n3.10112. *Corresponding author: ddhong60vn@yahoo.com Received 19 June 2017, accepted 20 August 2017
Establishment of a procedure for producing the anti-b monoclonal antibody from B4C10D9 hybridoma cell line
Authors: Trung Nguyen Thi , Hai Truong Nam
Published: April 2018

There are two the most important blood group system in blood transfusion, that is the ABO system and the Rh system. The anti-A or anti-B antibodies in the blood of recipient cause strongly agglutination the red blood cells of donor bearing A or B antigens on their surface respectively. So, all donated blood and received blood must be typed group for the ABO system to be safe in blood transfusion. To blood grouping, used of know antisera reacts with the whole blood samples to determine antigen on the surface of red blood cell or use of know red blood cells reacts with the individual's serum to determine antibody. The serology method is widely used, antisera are usually monoclonal antibodies secreted by hybridoma cells. Our previous studies showed that the B4C10D9 hybridoma cell line generated anti-B monoclonal antibody was produced by hybridoma technology. This antibody causes specificity agglutination the red blood cells caring B antigen on their surface. It is  classed as IgM antibody with it heavy chains are µ and κ light chains. The aim of the study was to produce the anti-B monoclonal antibody form B4C10D9 hybridoma cells line serve as a part of ABO blood grouping reagent. Based on results of experiments, procedure for producing the anti-B monoclonal antibody from B4C10D9 hybridoma cell line has also been established with following steps. At first, refresh the frozen B4C10D9 hybridoma cells and the density of B4C10D9 hybridoma cells is 4.0 x 104 cells/ml at 24 hours post-inoculation. Then the B4C10D9 hybridoma cells are splitted 1:5 every 48 hours for 2 times to obtain the cell biomass. In the next step, the hybridoma cells are splitted into 125 cm2 flasks with the initial cell density of 105 cells/ml. The B4C10D9 hybridoma cells density reach 9.9 x 106 cells/ml at 48 hours post-inoculation. After 144 hours of post-inoculation, collect culture medium and concentrate the anti-B monoclonal antibody by precipitation with saturated ammonium sulfate at a final concentration of 50%. If resuspending volume equals initial volume, then the anti-B monoclonal antibody titer reaches 1/256 and the antibody reaction intensity will be 3+. And if the obtained antibody solution volume equals one fifth of the original volume, then the anti-B monoclonal antibody titer reaches 1/1024, and the antibody reaction intensity will be 4+.   Citation: Establishment of a procedure for producing the anti-b monoclonal antibody from B4C10D9 hybridoma cell line. Tap chi Sinh hoc, 39(3): 342-348. DOI: 10.15625/0866-7160/v39n3.10765. *Corresponding author: tnhai@ibt.ac.vn Received 20 August 2017, accepted 20 September 2017
Life history of Chrysozephyrus alienus (Koiwaya & Monastyrskii, 2010) (Lepidoptera) in central Vietnam
Authors: Hasegawa Takashi , Saito Kotaro , Lien Vu Van , Miyagawa Takashi , Kubota Akiko , Yokochi Takashi
Published: April 2018

We have successfully reared the larvae of Chrysozephyrus alienus (Koiwaya & Monastyrskii, 2010), and presented the life history of this species. The larval foodplant was found to be Lyonia ovalifolia var. pubescens. The tactile setae of the 1st instar larvae are absent at dorsal anterior area at the 1st abdominal segment and dorsal area at the 7th abdominal segment. The pattern is rather similar to that of Chrysozephyrus scintillans.   Citation: Hasegawa T., Saito K., Vu Van Lien, Miyagawa T., Kubota A., Yokochi T., 2017. Life history of Chrysozephyrus alienus (Koiwaya & Monastyrskii, 2010) (Lepidoptera) in central Vietnam. Tap chi Sinh hoc, 39(3): 291-295. DOI: 10.15625/0866-7160/v39n3.9348. *Corresponding author: airsjapan64@gmail.com Received 20 March 2017, accepted 20 August 2017
Biodegradation of aniline by freely suspended and immobilized Pseudomonas moraviensis AN-5
Authors: Duc Ha Danh , Triet Bui Minh
Published: April 2018

Pseudomonas moraviensis AN-5 isolated from contaminated sludge could utilize aniline as a sole carbon and nitrogen source. The aniline degradation by cells immobilized in alginate, polyvinyl alcohol (PVA) and polyurethane foam (PUF) was significantly better than freely suspended cells. The immobilized AN-5 degraded aniline nearly completely at the initial concentration of 22 mM by 48 hr of incubation. Compared to other binding materials, PUF-immobilized cells were more stable in aniline biodegradation in reused cycles. Moreover, immobilized cells could be stored for three months at 4oC with little reduction of the aniline degradation capacity. These results revealed that PUF-immobilized P. moraviensis AN-5cells has enhanced aniline biodegradation activity.   Citation: Ha Danh Duc, Bui Minh Triet, 2017. Biodegradation of aniline by freely suspended and immobilized Pseudomonas moraviensis AN-5. Tap chi Sinh hoc, 39(3): 303-308. DOI: 10.15625/0866-7160/v39n3.9630. *Corresponding author: hadanhduc@gmail.com Received 11 April 2017, accepted 20 August 2017
The status of invasive plants and animals in Cu Lao Cham biosphere reserve, Quang Nam province, Vietnam
Authors: Tai Vu Anh , Khanh Uong Dinh , Anh Luu The , Thu Hien Le Thi
Published: April 2018

The biodiversity of Cu Lao Cham Biosphere Reserve (Hoi An City, Quang Nam Province) has been faced with some passive impacts, one of which is invasion/expansion of alien species. In 2017, according to the data of GISD, CABI and the Inter-ministerial Circular No.27/2013/TTLT-BTNMT-BNNPTNT, based on filed survey conducted in May, 19 alien plant and 3 alien animal species were recorded in the biosphere reserve. Among them, 13 plant species were identified as invaders, of which details were assessed in this study; among those invader plants, 3 species were ranked at medium risk and the 10 others were ranked at low risk. All of the medium risk-invasive plant species have been appeared on the islands but one of them - siam weed (Chromolaena odorata) were not identified as impacting to the mainland of the biosphere reserve. Likewise, all of the alien animal species have been not recognized as the invasive species. In general, the impact of alien species found in the Cu Lao Cham was assessed as “Low Risk”. The impact status of invasive species in the Hoi An mainland part is more serious than the situation in the islands. Base on the results, we suggest that, five species, beggar-ticks (Bidens pilosa), coast morning glory (Ipomoea cairica) Bay Biscayne creeping-oxeye (Sphagneticola trilobata), Blue porterweed (Stachytarpheta jamaicensis) and billygoat-weed (Ageratum conyzoides) should be added in the invasive appendix of the national invasive species list while three other species as vilfa stellata (Cynodon dactylon), guava (Psidium guava) and rose myrtle (Rhodomyrtus tomentosa) should be listed in the potential appendix of that list. It is necessary to conduct some survey to obtain solution to control invasive species as soon as possible to protect the biodiversity of this study area. Citation: Vu Anh Tai, Uong Dinh Khanh, Luu The Anh, Le Thi Thu Hien, 2017. The status of invasive plants and animals in Cu Lao Cham biosphere reserve, Quang Nam province, Vietnam. Tap chi Sinh hoc, 39(4): 434-450. DOI: 10.15625/0866-7160/v39n4.10082.*Corresponding author: tai.botany@gmail.comReceived 15 June 2017, accepted 12 December 2017
Degradation of leaves and cellulose by Bacillus subtilis CL3 and Pseudomonas aeruginosa CL5 isolated from left waste dumps in Vietnam
Authors: Duc Ha Danh
Published: April 2018

The biodegradation capacity of two bacterial strains, Bacillus subtilis CL3 and Pseudomonas aeruginosa CL5, isolated from left waste dumps on thee different plant leaves were investigated. The leaves were derived from perennial plants, Malabar almond tree (Terminalia catappa), eucalyptus (Eucalyptus terticornis) and African mahogany (Khaya senegalensis). Among those 3 plants, Eucalyptus terticornis is known to be resistant for biodegradation in nature. In our study, B. subtilis CL3 could degrade a half of Eucalyptus terticornis leaves within 10 days. On the other hand, P. aeruginosa CL5 showed relatively poor degradation rate of the leaves of Eucalyptus terticornis, but could effectively degrade Khaya senegalensis and Terminalia catappa leaves than B. subtilis CL3. When these two bacteria strains were mixed, the degradation rates of all these types of leaves were enhanced. While B. subtilis CL3 could utilize 1,8-cineole, a component extracted from Eucalyptus terticornis, as a sole carbon source, this component inhibited the growth of P. aeruginosa CL5. The multiple and efficient left degradation capacities make these isolates good candidates for bioremediation of leaf waste and cellulose. Citation: Ha Danh Duc, 2017. Degradation of leaves and cellulose by Bacillus subtilis CL3 and Pseudomonas aeruginosa CL5 isolated from left waste dumps in Vietnam. Tap chi Sinh hoc, 39(4): 483-488. DOI: 10.15625/0866-7160/v39n4.10744.*Corresponding author: hadanhduc@gmail.comReceived 5 December 2016, accepted 12 December 2017
Egg-lying behavior of Anisopteromalus calandrae (Howard), an Ectoparasitoid of Lasioderma serricorne (Fabricius)
Authors: Oanh Nguyen Thi , Lan Tran Ngoc , Lam Truong Xuan
Published: April 2018

This paper describes the egg-laying behavior and reproduction capacity of Anisopteromalus calandrae in laboratory conditions. Anisopteromalus calandrae lay eggs only on the Lasioderma serricorne larvae hiden inside of grains or nests. The maximum lifespan of A. calandrae females was 32 days when they were kept with of aqua feed flour plus honey (30%) and infested with larvae of Lasioderma serricorne. One female laid 71.13 ± 4.24 eggs. The eggs were laid mostly (81.95%) during the first half of the lifespan, with the peak of 7.40 eggs/day on the 9th day. The females usually lay one egg/host larva, but sometimes they lay 2-5 eggs/host larva. They prefer to lay eggs on the 4th larval instar. Citation: Nguyen Thi Oanh, Tran Ngoc Lan, Truong Xuan Lam, 2017. Egg-lying behavior of Anisopteromalus calandrae (Howard), an ectoparasitoid of Lasioderma serricorne (Fabricius). Tap chi Sinh hoc, 39(4): 416-420. DOI: 10.15625/0866-7160/v39n4.10935.* Corresponding author: ntoanh@dthu.edu.vn.Received 17 August 2017, accepted 20 November 2017
Performance and feed intake of beef heifers on rotational grazing of natural grassland receiving protein and energy supplement in cool season
Authors: Kuinchtner Bruno Castro , Quadros Fernando Luiz Ferreira de , Jochims Felipe , Casanova Pedro Trindade , Dutra Gabriela Machado , Ongaratto Fernando , Carvalho Régis Maximiliano Roos de
Published: April 2018

ABSTRACT: The aim of this study was to evaluate the performance and feed intake of grazing beef heifers on two grazing intervals determined by thermal sum related to leaf elongation duration during cool season on natural grasslands. A complete randomized block design experiment with two treatments and three replications was conducted from May to September 2011 in the central part of the state of Rio Grande do Sul, Brazil. The treatments were two thermal sums calculated at 375 and 750 degrees-days (DD) to determine the intervals between grazing periods in a rotational grazing system. Thirty six beef heifers with average age of 18 months old and (initial body weight = 220±14kg) were used, all heifers received ground corn supplement at a rate of 5g kg-1 of body weight per day, at 2p.m., throughout the experiment and had free access to mineral and protein supplementation (450g kg-1 of CP). Similar average daily gain (ADG), beef production gain (BPG) and feed intake (FI), were obtained in both treatments. Leaf elongation duration is an alternative grazing management tool for improved animal production and to increase animal stocking rate.
A CONSTITUIÇÃO DOS DISCURSOS SOBRE ENSINO DE LÍNGUA PORTUGUESA NAS DÉCADAS DE 1980 E 1990
Authors: Pietri Émerson de
Published: April 2018

RESUMO Neste trabalho, observam-se processos de constituição de discursos sobre ensino de língua portuguesa estabelecidos nas duas últimas décadas do século XX, em contexto brasileiro. Compõem o material de análise três documentos publicados por instâncias oficiais como subsídios, propostas ou parâmetros para o ensino de língua portuguesa: Criatividade e Gramática (FRANCHI, [1987] 1991); a Proposta Curricular para o Ensino de Língua Portuguesa (SÃO PAULO, 1988); e os Parâmetros Curriculares Nacionais (BRASIL, 1998). Analisam-se os processos de (re)definição dos conceitos de atividade linguística e de atividade epilinguística em documentos de orientação curricular para o ensino de língua portuguesa publicados no período mencionado, em função dos diferentes modos como concebida a relação entre ensino de língua portuguesa e as condições de produção de linguagem na escola. A partir da observação dos modos como os conceitos de atividade linguística e atividade epilinguística se redefiniram nos processos interdiscursivos analisados, elaboram-se hipóteses sobre quais reconfigurações discursivas se operaram para a constituição da língua portuguesa em objeto de pesquisa e de ensino no período histórico observado.
IDENTIDADES DOCENTES ENTRE MUNDOS DISCURSIVOS EM DISPUTA: FORMAÇÃO DO PROFESSOR, LETRAMENTOS E DESENVOLVIMENTO
Authors: Nascimento Elvira Lopes , Grande Paula Baracat De
Published: April 2018

RESUMO O presente artigo busca analisar as identidades docentes construídas, negociadas e disputadas no processo de inserção de uma professora da escola básica em um curso de Mestrado Profissional. Para isso, selecionamos excertos de uma sessão de autoconfrontação realizada após a conclusão do curso de formação. Partimos do arcabouço teórico do Interacionismo Sociodiscursivo e da Engenharia Didática da Escola de Genebra, perspectivas estudadas pela professora participante da pesquisa para a escrita de sua dissertação de mestrado. Para a análise das identidades, mobilizamos a perspectiva sociocultural dos Estudos de Letramento, o conceito de identidade como múltiplo, fluido e construído discursivamente, e o conceito de vozes sociais, para compreender se o processo de construção de identidades em mundos discursivos em disputa - a escola e a universidade - resulta em desenvolvimento profissional da professora. Os resultados salientam a importância de se considerar a formação como um processo identitário, que envolve uma construção do que é ser professor na contemporaneidade.
COMPETÊNCIA EM TRADUÇÃO E LÍNGUAS DE SINAIS: A MODALIDADE GESTUAL-VISUAL E SUAS IMPLICAÇÕES PARA UMA POSSÍVEL COMPETÊNCIA TRADUTÓRIA INTERMODAL
Authors: Rodrigues Carlos Henrique
Published: April 2018

RESUMO Neste artigo, oferecemos uma primeira reflexão sobre as prováveis implicações da modalidade de língua, no caso a gestual-visual, sobre a noção e a modelagem da competência tradutória. Para tanto, realizamos uma breve revisão de algumas definições e modelos de competência tradutória e apresentamos as características das línguas de modalidade gestualvisual, bem como seus impactos sobre a atuação dos tradutores/intérpretes de línguas de sinais. Vimos que considerar a modalidade gestual-visual na definição e modelagem da competência tradutória é uma questão extremamente complexa. Entretanto, é, ao mesmo tempo, relevante, já que as investigações nesse âmbito podem contribuir significativamente à melhor compreensão do que constituiria uma competência tradutória "universal" e, mais estritamente, do que comporia a competência dos tradutores e dos intérpretes de línguas de sinais. Concluímos que a competência requerida dos tradutores e intérpretes de línguas de sinais é marcada por certa capacidade corporal cinestésica, diretamente ligada à competência linguística e à competência comunicativa. E, portanto, poderia ser concebida como uma competência tradutória intermodal.
TENDENCIAS DEFORMANTES Y PARATEXTOS EN LA TRADUCCIÓN AL INGLÉS DE HUASIPUNGO, DE JORGE ICAZA
Authors: González María del Pilar Cobo , Bein Roberto
Published: April 2018

RESUMEN Huasipungo, de Jorge Icaza, es quizá la novela ecuatoriana más reconocida en el mundo. Esta obra, cuya primera versión fue escrita en 1934, refleja el sufrimiento de los indígenas huasipungueros ante los maltratos de los mestizos. Si bien Icaza utiliza varias estrategias para reflejar el sufrimiento de los indígenas y plasmar su forma de vida, el uso del lenguaje es la más efectiva, pues, mediante este, caracterizado por una marcada influencia del quichua andino, pretende dar voz a quienes no la tienen. Esta estrategia presenta una dificultad en el momento del traducir la novela a otros idiomas. En este trabajo se analizarán los rasgos que caracterizan a la traducción al inglés de Huasipungo (titulada The Villagers), realizada por Bernard Dulsey y publicada en 1964. Esta traducción se basa en la segunda versión de la novela, de 1953, pues Icaza la revisó y reformuló la prosa. Dulsey recurrió a varias estrategias que le permitieron mantener esta esencia. Se analizarán los paratextos de la traducción (el título, la introducción y el glosario) para entender cómo contribuyen para que el lector angloparlante se acerque a la problemática del indígena ecuatoriano de la década de los años treinta. No obstante, muchas características lingüísticas y culturales de los indígenas se pierden al traducirse al inglés. Estas pérdidas serán analizadas mediante una revisión de algunas de las tendencias deformantes que anota Berman en su texto 'La analítica de la traducción y la problemática de la deformación' (1999).
INA CÉSAIRE E OS CONTOS CRIOULOS MARTINICANOS: DESAFIOS DE TRADUÇÃO
Authors: Reuillard Patrícia Chittoni Ramos , Siqueira Samanta Vitória
Published: April 2018

RESUMO Este artigo apresenta um projeto de tradução do francês para o português do livro Contes de nuits et de jours aux Antilles (1989), da etnóloga e escritora martinicana Ina Césaire, e as reflexões sobre a tradução desses contos. A obra é o resultado do trabalho de Césaire enquanto etnóloga, reunindo 14 contos orais crioulos da Martinica que foram transcritos a partir de suas gravações de um conjunto mais vasto de contos, realizadas entre 1975 e 1988, no interior da ilha. O trabalho tem como objetivo apresentar a autora e sua obra e traçar um breve panorama da história literária e da identidade cultural martinicana. Como a oralidade e o imaginário crioulo são características bastante presentes nos contos, também se visa uma reflexão sobre esses temas.
CULTURAS LEGÍTIMAS: LA TRADUCCIÓN DE HISTORIETAS LATINOAMERICANAS EN BRASIL
Authors: Zocal Barbara
Published: April 2018

RESUMEN En este artículo, realizamos un sobrevuelo sobre cuestiones sociales de las últimas décadas con respecto al lugar de la traducción de la lengua española en Brasil. Además, tratamos de presentar la relevancia de la traducción en el mercado editorial de las historietas, sobre todo de las latinoamericanas traducidas al portugués, con el objetivo de observar las regularidades del mercado editorial de las historietas traducidas. Al final, presentamos un estudio de caso de la traducción brasilera, intitulada Sendero Luminoso (Editorial Veneta, 2016), de las novelas gráficas peruanas Rupay. Historias gráficas de la violencia política en el Perú (1980-1984), de Luis Rossell, Alfredo Villar y Jesús Cossio (Editorial Contracultura, 2008), y Barbarie. Comics sobre Violencia Política en el Perú, 1985-1990, de Jesús Cossio (Editorial Contracultura, 2010).
TEORIA CRÍTICA E DIDÁTICA: UM DESAFIO PARA A EDUCAÇÃO CONTEMPORÂNEA
Authors: Maranhão Carolina , Santos Flávia Carolini Pereira dos , Gouveia Pedro Nunes
Published: April 2018

RESUMO Este artigo se propõe a tecer reflexões sobre a semiformação, estado em que se encontra o ensino superior, apresentando a didática crítica de Gruschka (2014) como alternativa crítica, capaz de recuperar o potencial de esclarecimento da educação. Para tal, pautou-se a discussão através dos estudos críticos de Adorno (1996) em seu ensaio conhecido como a Teoria da Semicultura (1959). A massificação cultural invadiu o sistema educacional cuja formação técnica e racional se multiplicou e resultou na banalização de práticas que revelam a degradação de espaços - como a sala de aula - que originariamente deveriam permitir a capacidade da autorreflexão, em locais de doutrinação instrumental de artifícios que postulam os alunos às exigências do mercado e aos imperativos de uma sociedade totalmente imersa no consumismo. Criou-se uma formação na qual todos os interesses se aliaram ao capital - a ideia de que o sujeito quanto mais ajustado ao seu estilo de vida à lógica do capital, mais produtivo se tornava. Restou para a educação contemporânea o desafio de permanecer na reflexão crítica quando seu ambiente já está tomado pelo semiformação.