bluuebunny/crossref_metadata_2025 huggingface dataset:
An unknown early 19th c. historical source on the subsistence activities of the population of the Lower Irtysh River basin: analysis of the information potential
Authors: Adaev V.N. , Konev A.Yu.
Published: December 2023

This paper reports on a previously unpublished document — a survey of the traditional subsistence activities of the popu-lation of the Lower Irtysh River basin and the adjacent part of the Ob River (Denshchikov Commissariat of the Tobolsk Gover-norate, Russia), prepared in 1805 by a local official, D.S. Kochetovsky. The survey contains detailed information on fishing, hunting, and gathering practices of the Russian and Ugric populations, and it was intended for the preparation of the Governor's report to the Minister of Internal Affairs. The study is aimed at assessing the information potential of the historical source and determination of the socio-political context of its production. In the paper, a historical-ethnographic analysis of the document has been carried out against other sources of the 18th–19th centuries, and its main text with annotations is published. The document attests to a high level of competence of the government official in the subsistence economy of the region accountable to him, although that was not fully detailed knowledge. It is not replete with unique data, but at the same time confirms the deep histori-cal tradition and conservatism of the local subsistence economy in which the ethnically mixed population was occupied. Unlike most of the published sources of the second half of the 18th c., the survey describes the economy of the clearly defined area of Northwestern Siberia, notably, as a whole complex, with the seasonal distribution of activities. Particularly valuable is the infor-mation characterising the flexibility of the economic structure, depending on the annual specifics of the fluctuation of natural resources. The authors of the paper concluded that the document under study constitutes one of the early experiences of com-piling a survey of the economy at a lower managerial level, marking the formation of a new model of interaction between state institutions and local communities. The model was based upon the collecting of statistical and factual data on all Russian regions, which towards the middle of the 19th c. adopted by then a regular and formalised order.
A bibliometric evaluation of the publications on METTL3 mediated m6A from 2000 to 2022
Authors: Wang Yingji , Tan Xiaohua , He Yaqiong , Li Xuan’an
Published: December 2023

Background: Many studies have demonstrated the role of METTL3 and other proteins in the N6-adenylate methylation(m6A) of RNA. It plays a role in many diseases such as cancer and tumor. However, there is a lack of comprehensive and objective reports on m6A. Therefore, this study aims to visualize the publications to quantify and determine the current status and trends of m6A with METTL3 as the catalytic core in the past two decades.
Methods: Relevant studies published between 1 January 2000 and 24 November 2022 in English were retrieved from the Web of Science Core Collection. We used four scientometric tools (Citespace v6.1.R2, VOSviewer, Scimago Graphica and Microsoft Excel 2019) to quantify and identify the individual impact and cooperation information by analyzing annual publications, the collaboration of authors, co-citation of authors, source, journals and references, cluster and co-occurrence of keywords.
Results: A total of 1349 publications were retrieved and showed a rapid upward trend. Among the top ten journals and the top ten co-cited journals, there are many top journals and their sub journals such as NATURE, CELL, SCIENCE, which indicates the fruitful results in this field. China has the highest publications, while the United States has the most significant academic influence. Chinese researchers have a keen interest in this research field, and high-yield institutions, authors and publications account for most of the ranking. It can be seen from co-cited references and reference burst that the research on biomarker, diagnosis, RRID, YTHDC1, hepatitis B virus, osteogenic differentiation and other aspects has sprung up and continues. The research of m6A pays more attention to its significance in different diseases, such as inflammation and cancer, in addition to its mechanism and role in cells.
Conclusion: METTL3 mediated m6A has achieved fruitful results at the cellular and molecular levels. The research in this field is moving from the exploration of molecular mechanism to the refinement of inflammation, cancer and other diseases, and researchers also pay special attention to its clinical application value. As a biomarker or target, METTL3 is likely to play a role in disease early warning, prognosis prediction and treatment goals.
The transcriptional landscape underlying larval development and metamorphosis in the Malabar grouper (Epinephelus malabaricus)
Authors: Huerlimann Roger , Roux Natacha , Maeda Ken , Pilieva Polina , Miura Saori , Izumiyama Michael , Laudet Vincent , Ravasi Timothy
Published: December 2023

Teleost fishes typically have a bi-phasic life cycle with a transition between larval and juvenile phases called metamorphosis, which is known to be regulated by thyroid hormones (TH). However, other hormonal systems might be involved as it is the case in amphibians in which corticosteroids are interacting with TH pathways to trigger and regulate metamorphosis. Unfortunately, such interplay is poorly understood in teleost fishes. In order to investigate the potential involvement of these two hormonal pathways, we used the Malabar grouper (Epinephelus malabaricus) as a model system. We assembled a chromosome-scale genome and conducted a transcriptomic analysis of nine larval developmental stages. We studied the expression patterns of genes involved in TH and corticoid pathways, as well as four biological processes known to be regulated by TH in other teleost species: ossification, pigmentation, visual perception, and metabolism. Surprisingly, we observed an activation of many of the same pathways involved in metamorphosis at an earlier stage, suggesting an additional implication of these pathways in early larval development. Overall, our data reveal that on a common background (TH controlling metamorphosis) evolution is assembling species-specific peculiarities that allow to precisely align the molecular completion of metamorphosis with the ecological constraints.
Aggregated epigenetic profiles explain the intergenerational transmission of physical neglect with psychiatric, cognitive, and bonding effects.
Authors: León Inmaculada , Góngora Daylín , Rodrigo María José , Herrero-Roldán Silvia , López Maykel , Mitchell Colter , Fisher Jonah , Medina Yasser Iturria
Published: December 2023

BACKGROUND. Past experiences of maltreatment and life adversity have been shown to induce DNA methylation changes in adults, but less is known about their impact on mothers’ maladaptive neglectful parenting.
METHODS. We performed an epigenome-wide association study (EWAS) to investigate the role of DNA methylation levels in the intergenerational association between mothers’ past experience of childhood neglect and current maternal neglect behavior. Saliva DNA methylation was determined with the Illumina Human Methylation EPIC BeadChip. We used a trajectory inference method to derive a personalized aggregated epigenetic maternal neglect score (EMNs) from 138 mothers (n=51 in the neglect group; n=87 in the control non-neglect group) having young children. Differential methylation between groups was utilized to derive the EMNs, adjusting for education level, age, experimental variables, and blood cell types in saliva samples.
RESULTS. Structural equation modelling: X2 (29) =39.590; p=0.091; RMSEA=0.052, confirmed the EMNs as a mediator between the neglectful mothers’ past experience of physical neglect and current reports of psychopathological symptoms, poor cognitive integrity, and observed poor mother-child emotional availability. A third of the genes annotated to the CpGs that affect EMNs are related to cognitive impairment and neurodegenerative and psychopathological disorders.
CONCLUSIONS. EMNs is a novel index to assess the contribution of DNA methylation changes to the intergenerational transmission of neglectful parenting behavior and its negative effects. The evidence provided expands the possibilities for earlier interventions on the neglect condition to prevent and ameliorate the impact of maternal adversities on mother-child care, helping to break the cycle of maltreatment.
A new primordial noble gas component in the solar system discovered in Ryugu asteroid
Authors: Verchovsky Alexander , Abernethy Feargus , Anand Mahesh , Franchi Ian , Grady Monica , Greenwood Richard , Barber Simeon , Suttle Martin , Ito Motoo , Tomioka Naotaka , Uesugi Masayuki , Yamaguchi Akira , Kimura Makoto , Imae Naoya , Shirai Naoki , Ohigashi Takuji , Liu Ming-Chang , Uesugi Kentaro , Nakato Aiko , Yogata Kasumi , Yuzawa Hayato , Karouji Yuzuru , Nakazawa Satoru , Okada Tatsuaki , Saiki Takanao , Tanaka Satoshi , Terui Fuyuto , Yoshikawa Makoto , Miyazaki Akiko , Nishimura Masahiro , Yada Toru , Abe Masanao , Usui Tomohiro , Watanabe Sei-ichiro , Tsuda Yuichi
Published: December 2023

Ryugu is the C-type asteroid from which material was brought to Earth by the Hayabusa2 mission. A number of individual grains and fine-grained samples analysed so far for noble gases have indicated that solar wind and planetary (P1=Q) noble gases are present in Ryugu samples with concentrations higher than those observed in CIs, suggesting the former to be more primitive compared to the latter. We analysed three fine-grained samples from Ryugu, one of which contained a Xe concentration an order of magnitude higher (4x10-7 cc STP/g) than previously found in samples from Ryugu. Isotopically, this Xe resembles Q, but with a much stronger (by a factor of four) isotopic fractionation relative to solar wind and an 36Ar/132Xe ratio at least an order of magnitude lower than in P1. This new planetary primordial noble gas component (here termed P7) provides clues to constrain how the solar composition was fractionated to form the planetary components.
Copula lucentia sp. nov., a new box jellyfish (Cnidaria: Cubozoa: Carybdeida) from Western Mediterranean Sea
Authors: Bordehore Cesar , Fonfría Eva S. , Straehler-Pohl Ilka , Stampar Sérgio N. , Collins Allen G. , Hoverd William Alan
Published: December 2023

A new species of box jellyfish, Copula lucentia sp. nov., is described from El Campello, Alicante, Spain and compared to the holotype of Copula sivickisi that was successfully recovered after being lost for almost 60 years. So far, the only cubozoan species recorded in the Western Mediterranean was Carybdea marsupialis. The genus Copula just included the type species (Copula sivickisi) reported from different temperate, tropical and subtropical localities in the Pacific, Atlantic and Indian Oceans. Morphologically, this new species possesses the typical characteristics of the genus Copula (four adhesive pads on the apex and a vertical keyhole-shaped rhopalial niche ostium) but it can be differentiated from the C. sivickisi holotype by the velarial canal pattern. In C. lucentia the velarial canal roots taper towards the velarial rim and each root bears 1–2 narrow triangular canals with sharp tips, resembling a bird-beak. In contrast, the velarial canal roots of C. sivickisi bear 2 short, broad canals that increase breadth towards the velerial rim and split up into 3 to 6 lobed, finger-like canals with rounded tips, giving the canal pattern a paw-like appearance. Molecular analyses of mitochondrial cytochrome c oxidase subunit I (COI) and nuclear 16S ribosomal DNA genes show that there are even more Copula species than expected, but which will not be focused on here. This study highlights not only that there are species yet to be discovered in the Mediterranean Sea, one of the most studied seas worldwide, but that a revision of the genus Copula is necessary.
Design, Formulation, Evaluation and Statistical Optimization of Coated Floating Pulsatile Tablets for Modified Chronopharmacotherapy of Rheumatoid Arthritis
Authors: jyothirmayi pasam
Published: December 2023

Background
Rheumatoid arthritis is the condition needs early morning medication according to biorhythms. Hence, the main aim of designing Coated Floating Pulsatile Tablets (CFPT) of Tramadol hcl for statistical optimization and to perform in vitro and in vivo buoyancy evaluation for modified chronopharmacotherapy of rheumatoid arthritis.
Methods
The formulation was designed with the immediate core covered with a compression coat followed by the upper buoyant and immediate layer. The best formulations of core tablets were compression coated with polymer HPMC K15M, Ispaghula gum (IG), and Tamarindus gum (TG) to assign a suitable lag time for formulation. HPMC K100M, Ispaghula gum, and their combination were compressed as the buoyant layers. Statistical optimization was done on parameters such as BLT, TFT, %SI, and % CDR.
Results
Immediate release layer and core tablets were optimized with formulation IF2 which contains sodium starch Glycolate 8%, and then in compression coated buoyant formulation CFPT was optimized by DoE optimization showing BL8 exhibiting good floating behavior and lag time, drug release for a period of 11 hrs.
Conclusion
As the formulation showed delay in drug release in vitro and also showed in vitro buoyancy, hence nighttime administration could be beneficial to reduce the rheumatoid arthritis complications in the early morning.
Experimental Behaviour of Prestressed Concrete Beams Under Simultaneous Sustained Loading and Corrosion
Authors: Rinaldi Zila , Carlo Fabio Di , Meda Alberto , Molaioni Filippo
Published: December 2023

The paper presents an experimental study for the evaluation of the flexural response and failure mode of a prestressed concrete (PC) beam subjected to simultaneous sustained loads and corrosion. The obtained results are judged and discussed also through a comparison with the experimental outcomes on a reference sound PC beam, and a companion specimen subjected to artificial corrosion first, and then tested in bending. The three specimens are characterized by a 200 x 300 mm rectangular cross section, a total length of 3700 mm and a clear span of 2700 mm. The value of the sustained load, applied with a simultaneous accelerated corrosion process of the strands, was chosen, based on the result of the uncorroded reference beam, to achieve a crack width of about 0.2 mm. The flexural response of the tested element was monitored over a period of 70 days, up to failure, and showed to be highly dependent on the localization of the corrosion phenomena affecting the strands, especially when coinciding with the maximum bending moment position. The obtained results are finally compared to the ones obtained on a PC beam with the same geometry and material properties, firstly subjected to corrosion and then tested in bending. The differences in corrosion morphology and location, and in the failure mode of the strand confirm the importance of accounting for the combined effect of reinforcement corrosion and loading when assessing the structural performance of prestressed concrete beams.
Alleviating coral thermal stress via inoculation with quorum quenching bacteria
Authors: Yang Qingsong , Yang Bing , Yang Bin , Zhang Wenqian , Tang Xiaoyu , Sun Huiming , Zhang Yanying , Li Jie , Ling Juan , Dong Junde
Published: December 2023

In the background of global warming, coral bleaching induced by elevated seawater temperature is the primary cause of coral reef degradation. Coral microbiome engineering using the beneficial microorganisms for corals (BMCs) has become a hot spot in the field of coral reef conservation and restoration. Investigating the potential of alleviating thermal stress by quorum quenching (QQ) bacteria may provide more tools for coral microbial engineering remediation. In this study, QQ bacteria strain Pseudoalteromonas piscicida SCSIO 43740 was screened among 75 coral-derived bacterial strains, and its quorum sensing inhibitor (QSI) compound was isolated and identified as 2,4-di-tert-butylphenol (2,4-DTBP). Then, the thermal stress alleviating potential of QQ bacteria on coral Pocillopora damicornis was tested by a 30-day controlled experiment with three different treatments: control group (Con: 29°C), high temperature group (HT: 31°C), and the group of high temperature with QQ bacteria inoculation (HTQQ: 31°C + QQ bacteria). The results showed that QQ bacteria SCSIO 43740 inoculation can significantly mitigate the loss of symbiotic algae and impairment of photosynthesis efficiency of coral P. damicornis under thermal stress. In addition, QQ bacteria inoculation suppressed the coral microbial community beta-dispersion and improved the stability of microbial co-occurrence network under thermal stress. It was suggested that QQ bacteria inoculation can alleviate coral thermal stress via reshaping microbial interaction and maintain community stability of coral microbiome. This study provided new evidence for the probiotic function of QQ bacteria in corals, which shedding light on the development of new microbiological tools for coral reef conservation.
Biofabrication of iron oxide nanoparticles from green seaweed Ulva lactuca and screening for its insecticidal activity
Authors: S Ramya , M Narayanasamy , L Eaban Eamy G. , G Chandrasekar , R Palanivelan
Published: December 2023

The primary aim of the research was to utilize Ulva lactuca, green seaweed, to biofabricate iron oxide nanoparticles (FeNP) and evaluate their insecticidal efficacy against Tuta absoluta, a pest that primarily impact tomato commercial crop. For the purpose of characterising biosynthesized nanoparticles, tools such as UV-Vis spectroscopy, FT-IR, XRD, SEM, and TEM were used. Inferred from the results is that, the aggregation of iron oxide nanoparticles is confirmed by the colour change from yellow to dark brown. Additionally, spectra from the 200–1000 range are absorbed by UV–Vis spectroscopy. The FT-IR peaks correspond to the band at 778 cm in the Ulva lactuca extract and correspond to F-O stretches of Fe2O3, confirming the production of nanoparticles. The band at 2851 cm may be due to C-N stretching from unreacted impurities or CO2 in the sample compartment. According to a TEM image of iron nanoparticles, the majority of these particles are almost spherical in shape, and their average size was found to be between 50 and 250 nm. Pure iron oxide nanoparticles were recovered from Ulva lactuca by green synthesis. The prominent peaks in the range of 30 to 70θ indicated by XRD demonstrate the extraction of pure iron oxide nanoparticles from Ulva lactuca. The results of the insecticidal tests indicate that the synthesised iron oxide nanoparticles had a satisfactory death rate of 63% with an observed concentration of 77.32 ppm. The values for the LC50 are 97.25 and LC90 are 110.8 ppm. The values were compared one to the other with the control. The insect Tuta absoluta will be a possible target for control with iron oxide nanoparticles generated from Ulva lactuca.
A Dual-controlled Guest Release from Cages
Authors: Wang Ying , Yao Yuqing , Shao Chengyuan , Wang Shuwei , Gong Qiufang , Liu Jia , Jiang Hua
Published: December 2023

Despite having significant applications in the construction of controlled delivery systems with high anti-interference capability, dual-controlled molecular release has not yet been achieved based on small molecular/supramolecular entities. Herein, we report a dual-controlled release system based on coordination cages, for which releasing the guest from the cage demands synchronously altering the coordinative metal cations and the solvent. The cages, Hg5L2 and Ag5L2, are constructed via coordination-driven self-assembly of a corannulene-based ligand. While Hg5L2 shows a solvent-independent guest encapsulation in all the studied solvents, Ag5L2 is able to encapsulate the guests in only some of the solvents, such as acetone-d6, but will liberate the encapsulated guests in 1,1,2,2-tetrachloroethane-d2. Hg5L2 and Ag5L2 are interconvertible. Thus, the release of guests from Hg5L2 in acetone-d6 can be achieved, but requires two separate operations, including metal substitutions and a change of the solvent. In chemistry, dual-controlled systems such as this have the potential in programmable synthesis, cooperating with single-controlled guest release systems to sequentially release different reactants/catalysts.
Corticosteroids for improving patient relevant outcomes in HELLP syndrome: a systematic review and meta-analysis
Authors: Kasem Asmaa Fahmy , Alqenawy Hamdy Bakry , Elgendi Marwa Abdelmawla , Ali Radwa Rasheedy , Ahmed Rania Hassan Mostafa , Sorour Mohammad Nahel , Hegab Khadiga Mostafa Hussein , El-skaan Rania Gamal Anwar , Helw Rowyna Hany Mohamed El , Elsewefy Mohamed Sameh , Abdelrazek Maya Mahmoud , Elrefaey Yasser Mostafa Mahmoud , Albahaie Mohamed Yasser Galal , Salama Mohamed Hamed , Nabhan Ashraf Fawzy
Published: December 2023

Background: We conducted this updated systematic review to assess the effects of corticosteroids vs placebo or no treatment for improving patient-relevant outcomes in hemolysis, elevated liver enzymes and low platelets (HELLP) syndrome.
Methods: CENTRAL, MEDLINE/PubMed, Web of Science, and Scopus, from the date of inception of the databases to September 20, 2023 were searched. Reference lists of included studies and systematic reviews were thoroughly searched. We included RCTs that enrolled women with HELLP syndrome, whether antepartum or postpartum, to receive any corticosteroid versus placebo or no treatment. No language or publication date restrictions were made. We used a dual independent approach for screening titles and abstracts, full text screening, data extraction, and risk of bias assessment. Pairwise meta-analyses were conducted, where two or more studies met methodological criteria for inclusion. GRADE approach was used to assess certainty of evidence for the pre-specified outcomes.
Results: Fifteen trials (821 women) compared corticosteroids with placebo or no treatment. The effect of corticosteroids is uncertain for the primary outcome i.e., maternal death (risk ratio [RR] 0.77, 95% confidence interval [CI] 0.25 to 2.38, very low certainty evidence). The effect of corticosteroids is also uncertain for other important outcomes including pulmonary edema, dialysis, liver morbidity (hematoma, rupture, and failure), or perinatal death because of very low certainty evidence. Low certainty evidence suggests that corticosteroids have little or no effect on the need for platelet transfusion and may result in a slight reduction in acute renal failure.
Conclusions: In women with HELLP syndrome, the effect of corticosteroids vs placebo or no treatment is uncertain for patient-relevant outcomes including maternal death, maternal morbidity, and perinatal death. These uncertainties regarding this critical question should be addressed by adequately powered rigorous trials.
Systematic review registration: Center for Open Science, osf.io/yzku5
Synergic Light-Induced Electron Transfer and [4 + 4] Cycloaddition toward Switchable Photoluminescence and Single-Molecule Magnet Behavior in a Dy4 Cubane
Authors: Wang Guo-Ming , Gao Zhen-Ni , Wang Yu-Han , Li Jie , Wei Wu-Ji , Han Songde , Zhang Yi-Quan , Hu Ji-Xiang
Published: December 2023

Molecular materials possessing switchable magneto-optical properties are of great interest for their potential applications in spintronics and molecular devices. However, switching their photoluminescence (PL) and single-molecule magnet (SMM) behavior via light-induced structural changes still constitutes a formidable challenge. Herein, a series of cubane structures were synthesized via self-assembly of anthracene acid and rare-earth ions under hydrothermal conditions. Upon Xe lamp irradiation at room temperature, all complexes exhibited obvious photochromic phenomena and complete PL quenching, which were realized via the synergistic effect of photogenerated radicals and [4 + 4] photocycloaddition of Ac components, with the quenched PL showing the largest fluorescence intensity change (99.72%) in electron-transfer photochromic materials. A reversible decoloration process was unexpectedly achieved via mechanical grinding stemming from the relaxation of photogenerated radicals. Moreover, an SMM behavior in the Dy analog appeared after room-temperature irradiation owing to the photocycloaddition of Ac ligands and photogenerated stable radicals changed the electrostatic ligand field and magnetic couplings. This work realizes for the first time complete PL quenching and photoinduced SMM behavior via simultaneous modulation of photocycloaddition and photogenerated radicals in one system, providing a dynamical switch for the construction of multifunctional polymorphic optical response materials and optical storage devices.
Osimertinib-associated Membranoproliferative Glomerulonephritis: a Case Report
Authors: GUO HENRY , YEUNG EMILY , BRANLEY PAULINE , LING JONATHAN
Published: December 2023

Osimertinib is a third-generation epidermal growth factor receptor-directed tyrosine kinase inhibitor (EGFR-TKIs) that selectively binds to T790M mutation and used in EGFR-positive non-small cell lung cancer (NSCLC), which has shown to improve progression-free survival. While there have been reports of first- and second-generation EGFR-TKIs causing acute tubulointerstitial nephritis, there have been limited case reports of osimertinib-associated glomerular injury. We describe a case of osimertinib-associated membranoproliferative glomerulonephritis (MPGN) in a patient with advanced Stage IV NSCLC and severe acute kidney injury (AKI), that responded with osimertinib cessation and a course of oral prednisolone. Thus, we recommend a renal biopsy in patients with an acute kidney injury suspected to osimertinib use.
Enhancing fire resistance in wood with high-water retention silica gel: A promising flame-retardant solution
Authors: Fei Zhongbin , Zhang Yingnan , Wang Zhi , Duan Yiling , Zhang Bin
Published: December 2023

Wood is highly susceptible to fire due to its flammability, causing serious casualties and property damage; therefore, wood treated with fire retardants will be a major issue. In this work, the refractory properties of silica gel with a high water retention capacity were investigated. Testing the water retention while taking into account the characteristics of the silica gel by researching the impact of varied component concentrations. Furthermore, various analytical approaches were used to explore the flame-retardant mechanism of silica gel. According to the experimental findings, silica gel features an abundance of hydrophilic groups and amorphous silica, resulting in a boost in the number of water molecules on its surface. In addition, at high temperatures, silica gel also aids in the formation of a SiO2 layer and a charred layer that serve as an oxygen barrier. This layer protects the wood from combustion. At the same flame temperature, the overall weight degradation of wood treated with silica gel was decreased by 36% and 53%, suggesting that silica gel may prevent the decomposition of wood by heat response. Overall, the study provides valuable insights into the flame-retardant mechanism of silica gel and its potential application in enhancing the fire resistance of wood.
The demethylation of genes antagonized by TET1 in the regulation of the Wnt/β-catenin signaling pathway impacts the biological characteristics of colorectal cancer.
Authors: Wang Kaiyun , Ding Hui , Guo Kaiwen , Chen Qiongrong , Qiu Wenhong , Chen Siyang
Published: December 2023

The TET family is a ten-eleven translocation family of dioxygenases that oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) and other oxidation products to regulate DNA methylation. Our data revealed significant downregulation of TET1 expression in CRC issues and SW480 cells. The database highlighted mutations as the primary mode of alteration of TET1 in CRC. The bioinformatics analysis results revealed a significant association between TET1 and immune cell infiltration, while indicating that the expression levels of immune checkpoint-related genes in CRC tissues tend to be elevated in comparison to normal tissues. Upon transfection, overexpression of TET1 exerted a comprehensive inhibitory effect by suppressing cell proliferation, inducing apoptosis, hindering migration and invasion, arresting cell cycle progression, and attenuating the activity of the Wnt/β-catenin signaling pathway as well as in nuclear β-catenin expression. Overexpression of TET1 increased 5hmC levels while simultaneously decreasing 5mC levels. We revealed antagonistic genes SFRP2 and WIF1 within the Wnt/β-catenin signaling pathway, which have a significant increase in expression level and a decrease in hypermethylation level upon TET1 overexpression. In conclusion, TET1 exerts its antitumor function by inhibiting the activity of Wnt/β-catenin signaling pathways through demethylation of the antagonistic genes SFRP2 and WIF1. This modulation has a significant impact on the biological properties of CRC.
Analyze the epidemic, lagging effect and prediction in time series models, applied to monthly weather and pollution related HFRS
Authors: Hou Weiming
Published: December 2023

Background
Previous studies have typically explored daily lagged relationships among hemorrhagic fever with renal syndrome (HFRS) and meteorology, with little seasonal exploration of monthly lagged relationships, interactions and multiple predictions among hemorrhagic fever and pollutants.
Methods
Our researchers collected HFRS cases data from 2005–2018 as well as meteorological and contaminative factors from 2015–2018 for the Northeast region. Firstly, we reported the application of the moving epidemic method (MEM) to estimate epidemic threshold and intensity level. Then we developed a distributed lag non-linear model (DLNM) and generalized additive model (GAM) with a maximum lag of 6 months to evaluate the lagged and interaction effects of meteorological and pollution factors on HFRS cases. Multiple machine learning was then performed after applying Spearman analysis to screen environmental factors in the Northeast.
Results
There has been a yearly downward trend in the incidence of HFRS in the northeastern regions. High prevalence threshold years were in 2005–2007 and 2012–2014, the epidemic months were mainly concentrated in November. During the low prevalence threshold period, the main lag factor was low wind direction. And the meteorological lag effect was high during the high prevalence threshold period, where the main lag factors were cold air and hot dew point. Low levels of AQI, PM10 and high levels of PM2.5 showed a dangerous lag effect on the onset of HFRS, but a protective effect at extreme high levels of PM2.5. And high levels of AQI, PM10 and low levels of PM2.5 showed a protective lag effect. The model of PM2.5 and AQI interaction pollution is better. The SVM-Radial algorithm outperformed other algorithms, where the predictive variables of pollutants performed well.
Conclusions
This is the first mathematically based study of seasonal threshold of HFRS in Northeast China, which allows accurate estimation of epidemic level. Our findings support that long-term exposure to air pollution is the risk factor for HFRS. We should focus on pollutants monitoring in cold condition and HFRS prediction modeling.
A signature based on seven disulfidptosis-related long non‑coding RNAs to predict the prognosis in Wilms' tumor
Authors: Xie TianJu , Liu ZheZhe , Xia Yu , Ma KeXin , Wang YouJun
Published: December 2023

Disulfidptosis has been demonstrated to be associated with prognosis in certain tumors. However, the prognostic significance of disulfidptosis-related long non-coding RNAs (lncRNAs) in Wilms' tumor (WT) remains unclear. Initially, we downloaded the transcriptome and clinical data of WT patients from the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) database. Then, we identified disulfidptosis-related lncRNAs with prognostic significance to construct a risk model through correlation analysis, univariate Cox regression analysis, Lasso regression analysis, and multivariate Cox regression analysis. Furthermore, we validated the accuracy of the model using survival analysis, independent prognostic analysis, C-index analysis, receiver operating characteristic (ROC) curves, and a nomogram. Finally, the analysis of tumor microenvironment and immune function was conducted in samples from both high-risk and low-risk groups. A total of 7 lncRNAs were ultimately identified for the development of a prognostic model. Upon internal validation, this model exhibited remarkable efficacy in accurately discriminating between high-risk and low-risk patients, thereby enabling precise prognosis determination. Furthermore, notable statistical disparities were observed in the tumor microenvironment composition and immune function between the high-risk and low-risk groups' samples. In summary, we have successfully developed a prognostic risk model for lncRNAs associated with disulfidptosis in pediatric patients with Wilms’ tumor (WT). It played an important role in determining prognosis and in investigating new targets for tumor therapy.
Association between healthy lifestyle score and dyslipidemia; a population based study
Authors: Sadafi Sepehr , Azizi Ali , Najafi Farid , Pasdar Yahya
Published: December 2023

Background
The prevalence of dyslipidemia in Iran is high, making it important to identify its risk factors. However, the association between dyslipidemia and the healthy lifestyle index (HLS), which encompasses various individual behaviors, has not been extensively studied. Therefore, the aim of this study was to investigate the association between HLS and dyslipidemia.
Methods
A cross-sectional study was conducted on 6,690 participants from the baseline phase of the Ravansar non-communicable diseases study (RaNCD) cohort study. The HLS score was determined based on four lifestyle components: physical activity, diet, smoking status, and obesity. Binary logistic regression models were used to analyze the association between dyslipidemia and HLS quartiles.
Results
The average age of the participants was 45.76 ± 7.76 years, with 47.49% being male and 41.26% residing in rural areas. Anthropometric indices decreased across the HLS quartiles (Ptrend < 0.001). The intake of whole grains, fruits, vegetables, legumes, white meat, eggs, and nuts was significantly higher in the fourth quartile compared to the first quartile of HLS (Ptrend < 0.05). After adjusting for age, sex, socioeconomic status, drinking habits, and residence, the odds of dyslipidemia decreased in the second (OR: 0.66, 95% CI: 0.57–0.81), third (OR: 0.55, 95% CI: 0.47–0.64), and fourth quartiles of HLS (OR: 0.37, 95% CI: 0.30–0.46) compared to the first quartile (Ptrend < 0.001).
Conclusion
This study shows that a higher HLS may be associated with a lower likelihood of dyslipidemia. Adopting a healthy lifestyle that includes smoking cessation, regular physical activity, weight control, and a nutritious diet may help prevent dyslipidemia.
Image Classification Using Clip
Authors: Le Hang Thi Bich , Tran Vy Ngoc Nhat
Published: December 2023

The image classification problem using the CLIP method is particularly important in fields such as e-commerce, healthcare, security, etc. In this paper, we will introduce the application of ImageCLIP using the OpenCLIP model combined with ”Zero-shot” for object classification. We used the pre-trained model of OpenCLIP combined with the object class labels in the CIFAR-100 dataset to implement the ImageCLIP application.
Analysis of the use of anesthetic blocks versus local anesthesia infiltration in primary total knee arthroplasty surgery.
Authors: Gómez Silvia Gómez , Mata Julián Carlos Segura , Nalvaiz José Tomás Alcalá , García Felicito García-Álvarez , Zaldívar Clara Marín , Goiricelaya Amagoia Fernández de Gamarra
Published: December 2023

PURPOSE
The aim of this study is to analyze the efficacy of the use of a combined infiltration between popliteal artery and knee capsule (IPACK) anesthetic block together with selective saphenous nerve block, versus local infiltration with anesthetic in knee replacement surgery.
METHODS
A retrospective observational study was performed. There were reviewed 312 patients who underwent primary total knee prosthesis at our hospital between January 2019 and December 2022, regardless of the reason for the surgical indication. Between January 2019 and November 2021, 207 patients were performed local intraarticular anesthesia (LIA) during the surgery. Since November 2021 to December 2022, 105 patients received the combined nerve blocks (IPACK + ACB group). The average age in the LIA group was 72.9 years while in the blocks group it was 70.4 years. There were 44% of men in the LIA group and 53.3% in the blocks group.
The primary outcome was the presence of poorly controlled pain that requires opioid analgesic rescue in the postoperative period. Secondary outcomes included pain scores measured using the visual analog scale (VAS), range of motion assessed with a goniometer and hospital length stay.
RESULTS
There were no significant differences in age or sex patient distribution between the two groups.
Only one patient treated with anesthetic blocks required rescue analgesia with minor opioids. Patients treated with LIA required treatment with minor opioids in 28.5% of cases. There were not statistically significant differences (p = 0.0964) between VAS in the LIA group and in the blocks group in the first 24h, although patients treated with LIA presented less elevated VAS data (mean 2.3 versus 2.7). The range of motion was slightly greater (4.6º, statistically significant data, < 0.05) in the blocks group. There were statistically significant differences in terms of hospital stay (2.4 days in the blocks group and 2.8 days in the LIA group (p < 0.05).
CONCLUSION
In our series patients treated with anesthetic blocks showed better results with similar control of postoperative pain. However, more studies would be needed.
On the application of components manufactured with stereolithographic 3D printing in high vacuum systems
Authors: Radic Aleksandar , Lambrick Sam Morgan , Rhodes Sam , Ward David James
Published: December 2023

We report on a method for using stereolithographic (SLA) additive manufacturing to rapidly and cheaply prototype components for use in high-vacuum environments. We demonstrate the primary vacuum contaminant from freshly printed SLA plastics is water with no evidence of polymers out-gassing from the material and thus the vacuum performance can be controlled with simple treatments which do not involve surface sealing. An unbaked vacuum system containing SLA printed parts achieved 1.9e-8 mbar base pressure whilst retaining structural integrity and manufacturing accuracy. Preliminary results indicate that our method can be extended to achieve ultrahigh-vacuum compatibility by baking at higher temperatures. We further report on the effect of atmospheric exposure to components and present evidence to suggest that re-wetting occurs exclusively in the component skin layer, by showing that the bulk mass changes of the material is irreversible on the timescale investigated (< 2 weeks).
eHealth literacy and health-related quality of life: associations between eHEALS, EQ-5D-5L and health behaviours in a cross-sectional population survey
Authors: Zrubka Zsombor , Gulácsi László , Baji Petra , Kovács Levente , Tóth Barbara , Fodor Szabina , Péntek Márta
Published: December 2023

eHealth literacy has been been seen as an influencing factor of population health, while supporting evidence is mixed. We aimed to explore the association between eHealth literacy and health-related quality of life, with focus on the mediating effect of health behaviours and subgroup differences concerning health status and sociodemographic characteristics. We conducted a cross-sectional online survey in Hungary (N=1000) recording the eHealth literacy scale (eHEALS), EQ-5D-5L, lifestyle-related risks, perception of subjective lifestyle and health information seeking activity. Data were analysed via consistent partial-least squares structural equation modelling (cPLS-SEM). eHEALS had a small indirect effect on EQ-5D-5L (standardised b =0.031, p=0.002), mediated by subjective lifestyle (i.e., one perceives his/her lifestyle as healthier than others), but no direct or indirect effect via lifestyle-related risks of health information seeking. The association between lifestyle-related risks and eHEALS score was weak and fully mediated by health information seeking in the full sample (b =-0.035, p=0.009), but there was strong direct association between the eHEALS score and fewer lifestyle risks in respondents with poor health (EQ-5D-5L < 0.67; b =-0.294, p=0.040). Our findings provide clues for provide clues for public health strategies aiming to improve eHealth literacy in the general population.
Regional differences, dynamic evolution, and driving factors of high-quality medical resources in China’s ethnic minority areas
Authors: Hao Bangyan , Liang Binghua , Chen Zhuo , Huang Lifeng , Li Chengcheng
Published: December 2023

Background: In China, as people’s standard of living improves and the medical service system becomes more sophisticated, the demand for higher-quality and improved healthcare services is steadily rising. Inequality in health resource allocation (HRA) is more pronounced in ethnic minority areas (EMAs) than in developed regions. However, little research exists on high-quality medical resources (HQMRs) in China’s EMAs. Hence, we examined the spatiotemporal dynamic evolution of HQMRs in China’s EMAs from 2007 to 2021 and identified the main factors affecting their respective HQMR levels.
Methods: We selected tertiary hospitals to represent the quality of healthcare resources. We employed descriptive statistical techniques to analyze changes in the distribution of HQMRs from 2007 to 2021. We used the Dagum Gini coefficient and kernel density approach to analyse the dynamic evolution of HQMRs in China’s EMAs. We utilised the least squares dummy variable coefficient (LSDVC) to identify key factors affecting HQMR.
Results: The number of HQMRs in each EMA has risen annually. The average number of tertiary hospitals increased from 175 in 2007 to 488 in 2021. The results of the Dagum Gini coefficient revealed that the differences in the HQMR level in China’s EMAs have slowly declined, and intra-regional disparities have now become the primary determining factor influencing overall variations. The kernel density plot indicated that the HQMR level improved significantly during the study period, but bifurcation became increasingly severe. Using the LSDVC for analysis, we found that gross domestic product (GDP) per capita, the size of the resident population, and the number of students enrolled in general higher education exhibited a significant negative correlation with HQMR levels, while GDP and urbanisation rate had a significant promoting effect.
Conclusions: The HQMR level in EMAs has risen rapidly but remains inadequate. The differences in HQMR between regions have continued to narrow, but serious bifurcation has occurred. Policymakers should consider economic growth, education, and population size rather than simply increasing the number of HQMRs everywhere.
Design of BUCK DC-DC Converter Portable Charging Application for Electric Vehicles
Authors: Kumar Shubham , Subbaraj Sangeetha , Kingsly Saffrine
Published: December 2023

As electric vehicles (EVs) are becoming more prevalent in the current scenario. One of the significant obstacles is range anxiety which hasn’t been overcome because of the short range of a single charge. By employing an on-the-spot dynamic charging system using a charged external battery pack, it can be avoided. In this manuscript, the control algorithms i.e. Proportional-Integral-Derivative controller (PID) are modelled by ensuring the electrical and thermal characteristics of the conversion are within a predetermined and acceptable range. The State-of-Charge (SOC), battery current, and battery voltage are observed and monitored in Matlab Simulink. To obtain the pragmatic results, a basic Battery Management System (BMS), which implements the Coulomb Counting method, is also developed and simulated. The semiconductor devices used are thermally modeled to measure dissipation. This is a suggested design for an emergency-only lightweight portable rapid charger. This design does not replace the standard charging stations.
Objective assessment of bradykinesia in Parkinson's disease using a serious game
Authors: Mendes Luanne Cardoso , Alves Camille Marques , Marques Isabela Alves , Cabral Ariana Moura , Morère Yann , Andrade Adriano de Oliveira
Published: December 2023

Background: Bradykinesia is the main motor symptom of Parkinson's disease (PD). Its clinical assessment is based on scales, such as the Movement Disorder Society-Unified Parkinson's Disease Rating Scale (MDS-UPDRS). However, this type of assessment has limitations, such
as subjectivity and dependence on experienced assessors. Hence, this study aimed to objectively assess bradykinesia using a bee-themed serious game, so-called RehaBEElitation.
Methods: The experimental and control groups (EG and CG) consisted of fifteen individuals with PD in the ON and OFF states of medication, as well as fifteen healthy people, respectively, with matched ages and sexes. Bradykinesia was evaluated by detecting the voluntary movement of the gyroscope signals using Singular Spectrum Analysis (SSA), a time series decomposition method. The response time (RT) and angular velocity (AV) of the participants while playing RehaBEElitationwere estimated. The Kruskal Wallis test and Wilcoxon test with Bonferroni correction were used to confirm the differences between groups.
Results: The EG-OFF and CG participants who showed the worst results for RT also showed the worst results for AV, which reveals the consistency of the variables used to evaluate bradykinesia. Significant differences were found between groups for all comparisons (p<0.05). In most phases, CG participants obtained the best results for RT and AV, followed by EG-ON and then EG-OFF.
Conclusion: The RehaBEElitationserious game is an alternative tool for assessing the main motor symptom of PD in an objective and entertaining way. In general, the EG participants in the OFF state showed increased bradykinesia compared to the ON state.