bluuebunny/crossref_metadata_2025 huggingface dataset:
Significant increases in Donghong kiwifruit yield by a novel umbrella-shaped trellis system and identification of associated molecular mechanisms
Authors: Deng Honghong , Li Yao , Pang Changqing , Zhang Kun , Tian Xinbo , Wang Tong , Liang Yan , He Zunzhen , Lang Yuxuan , Fang Jinbao , Lin Lijin , Wang Jin , Lv Xiulan , Xia Hui , Liang Dong
Published: March 2023

China is the largest kiwifruit producer in the world, accounting for more than half of the total. However, in terms of yield per unit area, China is much lower than the global average and lags behind that of other countries. Yield improvement is of critical importance for the current kiwifruit industry in China. In this study, an improved overhead pergolas trellis (OPT) system, namely, the umbrella-shaped trellis (UST) system, was developed for Donghong kiwifruit, which is now the second most popular and widely cultivated red-fleshed kiwifruit in China. Surprisingly, the estimated yield on the UST system was more than two times higher than that with a traditional OPT, while the external fruit quality was maintained and the internal fruit quality was improved. One of the mechanisms contributing to the yield improvement was the significant promotion of the vegetative growth of canes at 6 ~ 10 mm in diameter by the UST system. The upper canopy of the UST treatment served as a natural shading condition for the lower fruiting canopy and thus had positive effects on the accumulation of chlorophylls and total carotenoids in the fruiting canopy. The most productive zones on the fruiting canes (6 ~ 10 mm in diameter) contained significantly higher (P< 0.05) levels of zeatin riboside (ZR) and auxin (IAA) and ratios of ZR/gibberellin (GA), ZR/abscisic acid (ABA), and ABA/GA. A relatively high carbon/nitrogen ratio may promote the flower bud differentiation process of Donghong kiwifruit. The outcomes of this study provide a scientific basis for manifold increase in production of kiwifruit and contribute to the sustainability of the kiwifruit industry.
Regulation of flavonoids in strawberry fruits by FaMYB5/FaMYB10 dominated MYB-bHLH-WD40 ternary complexes
Authors: Yue Maolan , Jiang Leiyu , Zhang Nating , Zhang Lianxi , Liu Yongqiang , Lin Yuanxiu , Zhang Yunting , Luo Ya , Zhang Yong , Wang Yan , Li Mengyao , Wang Xiaorong , Chen Qing , Tang Haoru
Published: March 2023

Anthocyanins endowing strawberry fruit red color are regulated by the MYB-bHLH-WD40 complex. By analyzing the MYBs involved in the flavonoid biosynthesis in strawberry, we found that R2R3-FaMYB5 promoted the content of anthocyanin and proanthocyanidins in strawberry fruits. Yeast two-hybrid and BiFC assays confirmed that MBW complexes connected with flavonoid metabolism were FaMYB5/FaMYB10-FaEGL3 (bHLH)-FaLWD1/FaLWD1-like (WD40). Transient overexpression and qRT-PCR analysis revealed that disparate MBW models hold different patterns in the regulation of flavonoid biosynthesis in strawberry fruits. Compared with FaMYB10, FaMYB5 and its dominant complexes showed a more specific regulatory range on strawberry flavonoid biosynthetic pathway, while FaMYB10 was more extensive. In addition, the complexes involved in FaMYB5 facilitated PAs accumulation primarily through the LAR tributary while FaMYB10 mainly by the ANR branch. FaMYB9 and FaMYB11 tremendously elicited the accumulation of proanthocyanidins by up-regulating the expression levels of both LAR and ANR, and also affected anthocyanin metabolism by changing the ratio of Cy3G and Pg3G which were constituted as two major anthocyanin monomers in strawberries. Our study also illustrated that FaMYB5-FaEGL3-FaLWD1-like directly targeted the promoters of F3′H, LAR, and AHA10 thus committing to flavonoid accumulation. These results allow the specific members involved in the MBW complex to be deciphered and provide new insights into the regulatory mechanisms of anthocyanins and proanthocyanidins regulated by the MBW complex.
Effects of various seed priming on morphological, physiological, and biochemical traits of rice under chilling stress
Authors: Zhang Hua , Zhang Xiaoli , Gao Guoqing , Ali Izhar , Wu Xiaoyan , Tang Maoyan , Chen Lei , Jiang Ligeng , Liang Tianfeng
Published: March 2023

Introduction/Background

Direct-seeded rice is exceptionally vulnerable to chilling stress, especially at the seed germination and seedling growth stages in the early season of the double cropping system.

Methods

Therefore, we conducted two experiments to evaluate the role of various seed primings and their different concentrations of plant growth regulators [experiment 1—abscisic acid (ABA), gibberellin (GA3), salicylic acid (SA), brassinolide (BR), paclobutrazol, uniconazole (UN), melatonin (MT), and jasmonic acid (JA)] and osmopriming substances (chitosan, polyethylene glycol 6000 (PEG6000), and CaCl2) and experiment 2—GA, BR (two best), CaCl2 (worst), and control (CK)] on rice seedlings under low temperature condition.

Results

Results showed that the maximum germination rate of 98% was recorded in GA3 (10 mgL−1) and BR (0.3 mgL−1) among treatments. Compared to CK, root and shoot length were improved in ABA (0.5 mgL−1) and GA3 (100 mgL−1) by 64% and 68%, respectively. At the same time, root and shoot weights (fresh and dry) were enhanced in Paclobutrazol (300 mgL−1) and GA3 among treatments. Furthermore, the average root volume, average root diameter, and total root surface area were increased by 27%, 38%, and 33% in Paclobutrazol (300 mgL−1), Paclobutrazol (200 mgL−1) and JA (1 mgL−1) treatments, respectively compared to CK. In the second experiment, a respective increase of 26%, 19%, 38%, and 59% was noted in SOD, POD, CAT, and APX enzyme activities in GA treatment compared to CK. Similarly, proline, soluble sugar, soluble protein, and GA content were also improved by 42%, 25.74%, 27%, and 19%, respectively, in GA treatment compared to CK. However, a respective reduction of 21% and 18% was noted in MDA and ABA content in GA treatment compared to CK. Our finding highlighted that better germination of primed-rice seedlings was associated with fresh and dry weights of the roots and shoots and the average root volume of the seedlings.

Discussion

Our results suggested that GA3 (10 mg L−1) and BR (0.3 mg L−1) seed priming prevent rice seedlings from chilling-induced oxidative stress by regulating antioxidant enzyme activities and maintaining ABA, GA, MDA, soluble sugar, and protein content. However, further studies (transcriptome and proteome) are needed to explore the molecular mechanisms involved in seed priming-induced chilling tolerance under field conditions.
The necessity of others: Entrepreneurial self-efficacy, TMT collective efficacy, CEO-TMT interface, and entrepreneurial orientation
Authors: Peng Xiaobao , Song Xiaofan , Horsey Emmanuel Mensah
Published: March 2023

Entrepreneurial orientation is the key factor for enterprises to obtain competitive advantages in dynamic circumstances. Thus, prior studies established the effect of psychological factors, for instance, entrepreneurial self-efficacy on entrepreneurial orientation using social cognitive theory. However, prior studies presented two main opposite views consisting of a positive and negative relationship between entrepreneurial self-efficacy and entrepreneurial orientation as well as providing no alleyway to enrich this relationship. We join the conversation on the positive linkage and argue on the essence of exploring the black box mechanisms to strengthen enterprises’ entrepreneurial orientation. We employed the social cognitive theory and collected 220 valid responses from CEOs and TMTs from 10 enterprises in the high-tech industrial development zones of nine provinces in China to clarify the effect of top management team (TMT) collective efficacy, and CEO–TMT interface on the link between entrepreneurial self-efficacy and entrepreneurial orientation. Our findings show that entrepreneurial self-efficacy positively affects entrepreneurial orientation. In addition, we found that a higher level of TMT collective efficacy strengthens the positive relationship between entrepreneurial self-efficacy and entrepreneurial orientation. Moreover, we discovered differential moderating effects. First, CEO-TMT interface positively affects entrepreneurial orientation when it interacts with TMT collective efficacy and entrepreneurial self-efficacy. Second, CEO-TMT interface has a significant negative indirect effect on entrepreneurial orientation, when it only interacts with TMT collective efficacy. Our study enriches the entrepreneurial orientation literature by positioning TMT collective efficacy and CEO-TMT interface as social cognitive mechanisms underlying the development of entrepreneurial self-efficacy and entrepreneurial orientation nexus. Thus, we open a window of opportunities for CEOs and decision-makers to maintain a sustainable position in the market, grasping more opportunities in uncertain conditions via timely entries into new markets and maintaining pre-existing ones.
Mothers’ and fathers’ engagement in math activities with their toddler sons and daughters: The moderating role of parental math beliefs
Authors: Silver Alex M. , Chen Yu , Smith Darcy K. , Tamis-LeMonda Catherine S. , Cabrera Natasha , Libertus Melissa E.
Published: March 2023

Parents’ beliefs about the importance of math predicts their math engagement with their children. However, most work focuses on mothers’ math engagement with preschool- and school-aged children, leaving gaps in knowledge about fathers and the experiences of toddlers. We examined differences in mothers’ and fathers’ (N = 94) engagement in math- and non-math activities with their two-year-old girls and boys. Parents reported their beliefs about the importance of math and literacy for young children and their frequency of home learning activities. Parents of sons did not differ in their engagement in math activities from parents of daughters. Mothers reported engaging more frequently in math activities with their toddlers than fathers did, but the difference reduced when parents endorsed stronger beliefs about the importance of math for children. Even at very early ages, children experience vastly different opportunities to learn math in the home, with math-related experiences being shaped by both parent gender and parents’ beliefs.
Characteristics and associated factors of violence in male patients with schizophrenia in China
Authors: Guo Weilong , Gu Yu , Zhou Jiansong , Wang Xiaoping , Sun Qiaoling
Published: March 2023

Objective

To investigate the characteristics and associated factors of violence in male patients with schizophrenia in China.

Methods

A total of 507 male patients with schizophrenia were recruited, including 386 non-violent and 121 violent patients. The socio-demographic information and medical history of the patients were collected. Psychopathological characteristics, personality traits psychopathology, and factors related to risk management were assessed using the Brief Psychiatric Rating Scale (BPRS), the History of Violence, Clinical, Risk Assessment Scale (HCR-20), and the Psychopathy Checklist-Revised (PCL-R), as appropriate. Differences in these factors were compared between the violent and non-violent patients, and logistic regression analysis was performed to explore the risk factors for violence in male patients with schizophrenia.

Results

The results showed that the violent group had a lower level of education, longer duration of illness, as well as a higher rate of hospitalization, history of suicidal attempts, and history of alcohol compared with the non-violent group. The violent group scored higher in items of symptoms in BPRS, personality traits and psychopathy in PCL-R, and risk management in HCR-20. The regression analysis showed that previous suicidal behavior (OR = 2.07,95% CI [1.06-4.05], P = 0.033), antisocial tendency in PCL-R (OR = 1.21, 95% CI [1.01-1.45], P = 0.038), H2: young age at violent incident (OR = 6.39, 95% CI [4.16-9.84], P < 0.001), C4: impulsivity (OR = 1.76, 95% CI [1.20-2.59], P = 0.004), and H3: relationship instability (OR = 1.60, 95% CI [1.08-2.37], P = 0.019) in HCR-20 were risk factors of violence among male patients with schizophrenia.

Conclusion

The present study found significant differences in socio-demographic information, history of treatment, and psychopathy characteristics between male patients with schizophrenia who had engaged in violent behaviors and their non-violent counterparts in China. Our findings suggested the necessity of individualized treatment for male patients with schizophrenia who had engaged in violent behaviors as well as the use of both HCR-20 and PCL-R for their assessment.
Occupational stress and burnout among intensive care unit nurses during the pandemic: A prospective longitudinal study of nurses in COVID and non-COVID units
Authors: Saravanan Pratima , Nisar Tariq , Zhang Qian , Masud Faisal , Sasangohar Farzan
Published: March 2023

Background

Intensive care unit (ICU) nurses are highly prone to occupational stress and burnout, affecting their physical and mental health. The occurrence of the pandemic and related events increased nurses’ workload and further exacerbated their stress and burnout. This work investigates occupational stress and burnout experienced by ICU nurses working with COVID and non-COVID patients.

Method

A prospective longitudinal mixed-methods study was conducted with a cohort of ICU nurses working in medical ICU (COVID unit; n = 14) and cardiovascular ICU (non-COVID unit; n = 5). Each participant was followed for six 12-h shifts. Data on occupational stress and burnout prevalence were collected using validated questionnaires. Physiological indices of stress were collected using wrist-worn wearable technologies. Participants elaborated on the causes of stress experienced each shift by completing open-ended questions. Data were analyzed using statistical and qualitative methods.

Results

Participants caring for COVID patients at the COVID unit were 3.71 times more likely to experience stress (p < 0.001) in comparison to non-COVID unit participants. No differences in stress levels were found when the same participants worked with COVID and non-COVID patients at different shifts (p = 0.58) at the COVID unit. The cohorts expressed similar contributors to stress, based in communication tasks, patient acuity, clinical procedures, admission processes, proning, labs, and assisting coworkers.

Conclusion

Nurses in COVID units, irrespective of whether they care for a COVID patient, experience occupational stress and burnout.
Availability and readiness of primary healthcare facilities for the management of non-communicable diseases in different districts of Punjab, Pakistan
Authors: Rashid Sadaf , Mahmood Humaira , Asma Iftikhar Asma , komal Nimrah , Butt Zikria , Mumtaz Hassan , Shellah Duha
Published: March 2023

Introduction

Non-communicable diseases (NCDs) and their effects are rising quickly. NCDs such as cardiovascular illnesses, diabetes, cancer, and chronic lung diseases cause 60% of global deaths; of which, 80% occur in developing countries. In established health systems, primary healthcare handles most of the NCD care.

Methodology

This is a mixed-method study conducted to analyze the health service availability and readiness toward NCDs using the SARA tool. It included 25 basic health units (BHUs) of Punjab, which were selected through random sampling. Quantitative data were collected using the SARA tools, while qualitative data were collected through in-depth interviews with healthcare providers working at the BHUs.

Results

There was a problem of load shedding of both electricity and water in 52% of the BHUs, which leads to the poor availability of healthcare services. Only eight (32%) out of 25 BHUs provide the diagnosis or management of NCDs. The service availability was the highest for diabetes mellitus (72%), followed by cardiovascular disease (52%) and then chronic respiratory disease (40%). No services were available for cancer at the BHU level.

Conclusion

This study raises issues and questions about the primary healthcare system in Punjab in two areas: first, the overall performance system, and second, the readiness of basic healthcare institutions to treat NCDs. The data show that there are many persisting primary healthcare (PHC) deficiencies. The study found a major training and resource deficit (guidelines and promotional materials). Therefore, it is important to include NCD prevention and control training in district training activities. NCDs are underrecognized in primary healthcare (PHC).
Implementing interventions to promote spectacle wearing among children with refractive errors: A systematic review and meta-analysis
Authors: Wu Linrong , Feng Jiayi , Zhang Mingzhi
Published: March 2023

Purpose

To investigate the level of compliance of children with refractive errors who are provided free spectacles, and to identify the reasons for non-compliance.

Methods

We systematically searched the PubMed, EMBASE, CINAHL, Web of Science, and Cochrane Library databases from the time these databases were established to April 2022, including studies published in English. The search terms were “randomized controlled trial” [Publication Type] OR “randomized” [Title/Abstract], OR “placebo” [Title/Abstract]) AND ((“Refractive Errors”[MeSH Terms] OR (“error refractive” [Title/Abstract] OR “errors refractive” [Title/Abstract] OR “refractive error” [Title/Abstract] OR “refractive disorders” [Title/Abstract] OR “disorder refractive” [Title/Abstract] OR “disorders refractive” [Title/Abstract] OR “refractive disorder” [Title/Abstract] OR “Ametropia” [Title/Abstract] OR “Ametropias” [Title/Abstract])) AND (“Eyeglasses” [MeSH Terms] OR (“Spectacles” [Title/Abstract] OR “Glasses”[Title/Abstract]) AND (“Adolescent” [MeSH Terms] OR (“Adolescents” [Title/Abstract] OR “Adolescence”[Title/Abstract]) OR “Child”[MeSH Terms] OR “Children”[Title/Abstract])). We only selected studies that were randomized controlled trials. Two researchers independently searched the databases, and 64 articles were retrieved after the initial screening. Two reviewers independently assessed the quality of the collected data.

Results

Fourteen articles were eligible for inclusion, and 11 studies were included in the meta-analysis. The overall compliance with spectacle use was 53.11%. There was a statistically significant effect of free spectacles on compliance among children (OR = 2.45; 95% CI = 1.39–4.30). In the subgroup analysis, longer follow-up time was associated with significantly lower reported ORs (6–12 vs. <6 months, OR = 2.30 vs. 3.18). Most studies concluded that sociomorphic factors, RE severity, and other factors contributed to children not wearing glasses at the end of the follow-up.

Conclusion

The combination of providing free spectacles along with educational interventions can lead to high levels of compliance among the study participants. Based on this study's findings, we recommend implementing policies that integrate the provision of free spectacles with educational interventions and other measures. In addition, a combination of additional health promotion strategies may be needed to improve the acceptability of refractive services and to encourage the consistent use of eyewear.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=338507, identifier: CRD42022338507.
Cardiovascular health research priorities in the United Arab Emirates
Authors: Ghader Nariman , Al-Yateem Nabeel , Dalibalta Sarah , Razzak Hira Abdul , Rahman Syed Azizur , Al Matrooshi Fatima , Al Shaya Sara , Al Marzouqi Amina
Published: March 2023

Background

Cardiovascular diseases (CVDs) are a leading cause of morbidity and mortality in the United Arab Emirates (UAE) and have been prioritized for intervention by healthcare authorities and clinicians.

Aim

To identify clinically relevant research priorities for the treatment and prevention of CVDs in the UAE.

Methods

This study used the nominal group technique to identify CVD-related research priorities. Participants were 37 experts from UAE hospitals, academic and research institutions, CVD associations, and paramedical organizations.

Results

Initially, 138 research topics were suggested by participating experts. These topics were then refined to identify the most important research priorities related to CVD prevention and treatment. The top research priority areas were: development of evidence-based, customized algorithms for CVD prevention and in-hospital emergency interventions; the availability, accessibility, and affordability of CVD treatment and rehabilitation; identification of relationships between CVDs, lifestyle factors, and mental health; efficacy and constraints in the management of cardiac emergencies; and epidemiological studies that trace CVD in the UAE.

Conclusion

The identified research priorities will guide a more informed research program for CVD treatment and prevention in the UAE. Funding opportunities and support for researchers should be prioritized for these identified research areas.
Cost-effectiveness of first line nivolumab-ipilimumab combination therapy for advanced non-small cell lung cancer: A systematic review and methodological quality assessment
Authors: Zaim Remziye , Redekop W. Ken , Uyl-de Groot Carin A.
Published: March 2023

To assess the methodological quality of cost-effectiveness analyses (CEA) of nivolumab in combination with ipilimumab, we conducted a systematic literature review in the first-line treatment of patients with recurrent or metastatic non-small cell lung cancer (NSCLC), whose tumors express programmed death ligand-1, with no epidermal growth factor receptor or anaplastic lymphoma kinase genomic tumor aberrations. PubMed, Embase, and the Cost-Effectiveness Analysis Registry were searched, in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. The methodological quality of the included studies was assessed by the Philips checklist and the Consensus Health Economic Criteria (CHEC) checklist. 171 records were identified. Seven studies met the inclusion criteria. Cost-effectiveness analyses differed substantially due to the applied modeling methods, sources of costs, health state utilities, and key assumptions. Quality assessment of the included studies highlighted shortcomings in data identification, uncertainty assessment, and methods transparency. Our systematic review and methodology assessment revealed that the methods of estimation of long-term outcomes, quantification of health state utility values, estimation of drug costs, the accuracy of data sources, and their credibility have important implications on the cost-effectiveness outcomes. None of the included studies fulfilled all of the criteria reported in the Philips and the CHEC checklists. To compound the economic consequences presented in these limited number of CEAs, ipilimumab's drug action as a combination therapy poses significant uncertainty. We encourage further research to address the economic consequences of these combination agents in future CEAs and the clinical uncertainties of ipilimumab for NSCLC in future trials.
SwarmFACE: A Python package for field-aligned currents exploration with Swarm
Authors: Blagau Adrian , Vogt Joachim
Published: March 2023

The SwarmFACE package utilizes magnetic field measurements by the Swarm satellites to study systems of field-aligned currents (FACs). Improvements of well-established techniques as well as novel single- and multi-satellite methods or satellite configurations are implemented to extend the characterization of FAC systems beyond the Swarm official Level-2 FAC product. Specifically, the included single-satellite algorithm allows to consider the FAC sheet inclination with respect to the satellite orbit and can work with low- or high-resolution data. For dual-satellite FAC estimation the package provides three algorithms, based on the least-squares, on the singular value decomposition, and on the Cartesian boundary-integral methods. These algorithms offer advantages over the corresponding Level-2 algorithm by providing more stable solutions for ‘extreme’ configurations, e.g. close to the orbital cross-point, and by allowing for a more general geometry of the spacecraft configuration. In addition, the singular value decomposition algorithm adapts itself to the spacecraft configuration, allowing for continuous, dual-satellite based FAC solutions over the entire polar region. Similarly, when Swarm forms a close configuration, the package offers the possibility to estimate the FAC density with a three-satellite method, obtaining additional information, associated to a different (larger) scale. All these algorithms are incorporating a robust framework for FAC error assessment. The SwarmFACE package further provides useful utilities to automatically estimate the auroral oval location or the intervals when Swarm forms a close configuration above the auroral oval. In addition, for each auroral oval crossing, a series of FAC quality indicators, related to the FAC methods’ underlying assumptions, can be estimated, like the current sheet inclination and planarity or the degree of current sheet stationarity.
Conceptual Model of Predictive Safety Management Methodology in Aviation
Authors: Bartulović Dajana , Steiner Sanja
Published: March 2023

Due to the continuous growth of air traffic and the development of aviation systems, the current safety management methodologies should be improved and upgraded. Safety management systems help aviation organizations to manage, maintain and increase safety efficiently. The focus of the research is on the development of the predictive safety management methodology to upgrade current reactive and proactive safety management methodologies and to improve the overall safety level in aviation organizations. Predictive methods are used in various aviation sectors (air navigation services, airport operations, airline operations) for planning purposes but not in the segment of safety management. Available examples of predictive methods were tested and analyzed. Time series decomposition methods were selected as most suited for implementation in aviation safety management. The paper explicitly emphasizes correlations between safety management methodologies in the sample aviation organization. The paper also shows how causal links among organizational and safety performance indicators can be detected, by developing causal models of mutual influences using causal modeling methods, on the sample organization. This research defined steps and tools of the conceptual model of predictive safety management methodology, which enables an organization to identify and mitigate future adverse events.
Aerocapture: Enabling Small Spacecraft Direct Access to Low-Circular Orbits for Planetary Constellations
Authors: Girija Athul Pradeepkumar , Saikia Sarag J. , Longuski James M.
Published: March 2023

Small satellite constellations in multiple-inclination, low-circular orbits around Mars and Venus have the potential to perform a range of high-value science investigations within cost-constrained missions. A major challenge for small satellites is that they require large ΔV to enter low-circular orbits, which can drive up both spacecraft mass and cost. Compared to chemical propulsion, which requires large amounts of propellant, and electric propulsion, which requires large solar arrays and comes with long flight times, aerocapture enables direct access to low-circular orbits at Mars and Venus with minimal ΔV. The study shows how drag-modulation aerocapture, when combined with small B-plane targeting maneuvers, allows the delivery of multiple small satellites to various-inclination, low-circular orbits to establish a constellation. Preliminary cost estimates indicate that by reducing the required ΔV for orbit insertion, aerocapture can potentially reduce the cost of a small satellite going to a low-circular Mars orbit compared to propulsive insertion. The ability of low-cost spacecraft to enter planetary orbits will enable a new paradigm of interplanetary missions using small dedicated launch vehicles and planetary constellations at Mars and Venus.
Breeding Milestones Correspond with Changes to Wheat Rhizosphere Biogeochemistry That Affect P Acquisition
Authors: McGrail Rebecca K. , Van Sanford David A. , McNear David H.
Published: March 2023

Breeding wheat (Triticum aestivum L.) has resulted in small gains in improved nutrient acquisition and use as numerous traits are involved. In this study, we evaluated the impact of breeding on P-acquisition and identified both plant and soil variables that could be used to inform the selection of germplasm with increased P acquisition efficiency. We previously screened a historic panel of winter wheat cultivars for root system architecture and root tip organic acid content when grown in P-deficient solution/agar and used these characteristics together with breeding history to develop a predicted P extraction potential (PEP). We tested the validity of the PEP classification by growing cultivars under sufficient and insufficient soil P conditions. Old, wild-type cultivars had the greatest P utilization efficiency (PUtE) when grown under insufficient P, likely a result of the chemical potential of wild-type (with respect to Rht-B1) cultivars (greater organic acid production) rather than root system size. Wild-type plants had differences in rhizosphere microbial community structure, rhizosphere bicarbonate-extractable P, and bulk soil Fe and Al, indicating the utilization of typically less available P pools. The PEP classification based on the presence of dwarfing allele and era of release offers a path forward for breeding for improved P acquisition.
Microbiome–Metabolome Reveals the Contribution of the Gut–Testis Axis to Sperm Motility in Sheep (Ovis aries)
Authors: Wang Mingming , Ren Chunhuan , Wang Penghui , Cheng Xiao , Chen Yale , Huang Yafeng , Chen Jiahong , Sun Zhipeng , Wang Qiangjun , Zhang Zijun
Published: March 2023

A close association exists among testicular function, gut microbiota regulation, and organismal metabolism. In this study, serum and seminal plasma metabolomes, and the rumen microbiome of sheep with significant differences in sperm viability, were explored. Serum and seminal plasma metabolomes differed significantly between high-motility (HM) and low-motility (LM) groups of sheep, and 39 differential metabolites closely related to sperm motility in sheep were found in seminal plasma metabolomes, while 35 were found in serum samples. A 16S rRNA sequence analysis showed that the relative abundance of HM and LM rumen microorganisms, such as Ruminococcus and Quinella, was significantly higher in the HM group, whereas genera such as Rikenellaceae_RC9_gut_group and Lactobacillus were enriched in the mid-LM group. Serum hormone assays revealed that serum follicle-stimulating hormone (FSH) and MT levels were significantly lower in the LM group than in the HM group, whereas serum glucocorticoid (GC) levels were higher in the LM group than in the HM group, and they all affected sperm motility in sheep. Ruminococcus and other rumen microorganisms were positively correlated with sperm motility, whereas Lactobacillus was negatively correlated with FSH and GCs levels. Our findings suggest that rumen microbial activity can influence the host metabolism and hormone levels associated with fertility in sheep.
Advancing DNA Barcoding to Elucidate Elasmobranch Biodiversity in Malaysian Waters
Authors: Loh Kar-Hoe , Lim Kean-Chong , Then Amy Yee-Hui , Adam Serena , Leung Amanda Jhu-Xhin , Hu Wenjia , Bong Chui Wei , Wang Aijun , Sade Ahemad , Musel Jamil , Du Jianguo
Published: March 2023

The data provided in this article are partial fragments of the Cytochrome c oxidase subunit 1 mitochondrial gene (CO1) sequences of 175 tissues sampled from sharks and batoids collected from Malaysian waters, from June 2015 to June 2022. The barcoding was done randomly for six specimens from each species, so as to authenticate the code. We generated barcodes for 67 different species in 20 families and 11 orders. DNA was extracted from the tissue samples following the Chelex protocols and amplified by polymerase chain reaction (PCR) using the barcoding universal primers FishF2 and FishR2. A total of 654 base pairs (bp) of barcode CO1 gene from 175 samples were sequenced and analysed. The genetic sequences were blasted into the NCBI GenBank and Barcode of Life Data System (BOLD). A review of the blast search confirmed that there were 68 valid species of sharks and batoids that occurred in Malaysian waters. We provided the data of the COI gene mid-point rooting phylogenetic relation trees and analysed the genetic distances among infra-class and order, intra-species, inter-specific, inter-genus, inter-familiar, and inter-order. We confirmed the addition of Squalus edmundsi, Carcharhinus amboinensis, Alopias superciliosus, and Myliobatis hamlyni as new records for Malaysia. The establishment of a comprehensive CO1 database for sharks and batoids will help facilitate the rapid monitoring and assessment of elasmobranch fisheries using environmental DNA methods.
Influence of the Season on the Nutritive Value and Gas Production of Opuntia ficus-indica and Agave americana L. in Ruminant Feed
Authors: Dias Cristiana S. A. M. Maduro , Nunes Helder P. B. , Vouzela Carlos F. M. , Madruga João S. , Borba Alfredo E. S.
Published: March 2023

Using invasive plants in animal production can provide an economical and eco-sustainable competitive advantage in a globalized market. The Opuntia ficus-indica and the Agave americana L. are invasive plants historically used by Azorean farmers as an alternative ruminant water source in the summer. This study aims to better understand their properties and how they vary throughout the year, so their use to complement animal diets can be optimised. Six samples of each species were collected on the Terceira Island during 2 growth seasons: winter (January 2021) and summer (September 2021), and their chemical composition, in vitro digestibility, and gas production, were determined. Significant (p < 0.05) differences were found in all parameters between the summer and the winter, with larger variations in both fibre (NDF and ADF) and digestibility parameters found between the Opuntia and the Agave. Gas production was greater in the summer for Opuntia and in the winter for Agave. Even though the digestibility was lower in the summer, we found that both plants offer a viable ruminant feed complement in both seasons, providing a cheap and eco-sustainable alternative water source, that can be associated to dry forage and compound feeding stuff with a high crude protein content when designing ruminant diets.
“Do Your Homework as Your Heart Takes over When You Go Looking”: Factors Associated with Pre-Acquisition Information-Seeking among Prospective UK Dog Owners
Authors: Mead Rebecca , Holland Katrina E. , Casey Rachel A. , Upjohn Melissa M. , Christley Robert M.
Published: March 2023

The factors influencing why and how people decide to acquire a dog are not well understood and little is known about the extent to which prospective owners undertake preparatory research. This study aimed to better understand what factors influence whether prospective dog owners in the UK conduct preparatory research. A 2019 online survey of current (n = 8050) and potential (n = 2884) dog owners collected quantitative and qualitative data. Additional qualitative data were collected through semi-structured interviews with current (n = 166) and potential (n = 10) dog owners. Of the current owners surveyed, 54% stated that they had looked for advice or information prior to acquiring their dog. Of potential owners, 68% reported already having looked for information, while a further 14% were planning to undertake research prior to acquiring a dog. Those with previous dog ownership experience were less likely to undertake pre-acquisition research, as were those who had worked with dogs. Demographic factors were also associated with the likelihood of conducting pre-acquisition research, with younger prospective owners being more likely to have undertaken research, as well as those with formal education qualifications. Among current owners, pre-acquisition research was more likely among those who acquired their dog through a breeder; a specific breed or a mix of two breeds; or as a puppy. Qualitative data were consistent with and added additional understanding and context to these findings. Almost half of current owners did not conduct pre-acquisition research, highlighting the need for increased awareness of its importance and the development of targeted interventions to encourage this activity. Understanding the different factors that influence whether dog owners undertake research may be of interest to animal welfare and veterinary organisations, in order to inform interventions to better prepare people for dog acquisition.
Shrews Under-Represented in Fruit Farms and Homesteads
Authors: Balčiauskas Linas , Stirkė Vitalijus , Garbaras Andrius , Balčiauskienė Laima
Published: March 2023

Shrews are a less studied group of small mammals than rodents. Between 2018 and 2022, we surveyed 23 sites in Lithuania, including natural and anthropogenic habitats, with the aim to assess the proportion of Soricidae in small mammal communities and their diet based on stable isotope analysis. The average representation of Soricidae was 3.1%, about half the long-term average in other habitats in the country. The highest proportions were in meadows and farmsteads, at 4.9% and 5.0% respectively. Shrews were not trapped on farms or in young orchards, and their relative abundance was very low in intensively managed orchards (0.006 individuals per 100 trap days). Neomys fodiens and N. anomalus were unexpectedly found in homesteads, including in outbuildings. Sorex araneus and S. minutus had similar diets. The trophic carbon/nitrogen discrimination factor between invertebrates and shrew hair was 2.74‰/3.98‰ for S. araneus, 1.90‰/3.78‰ for S. minutus in the orchards. The diet of N. fodiens and N. anomalus at the homesteads requires further investigation. We propose that the under-abundance of shrews may be due to contamination by plant protection products and a lack of invertebrates under intensive agricultural practices.
Identifying Malignant Breast Ultrasound Images Using ViT-Patch
Authors: Feng Hao , Yang Bo , Wang Jingwen , Liu Mingzhe , Yin Lirong , Zheng Wenfeng , Yin Zhengtong , Liu Chao
Published: March 2023

Recently, the Vision Transformer (ViT) model has been used for various computer vision tasks, due to its advantages to extracting long-range features. To better integrate the long-range features useful for classification, the standard ViT adds a class token, in addition to patch tokens. Despite state-of-the-art results on some traditional vision tasks, the ViT model typically requires large datasets for supervised training, and thus, it still face challenges in areas where it is difficult to build large datasets, such as medical image analysis. In the ViT model, only the output corresponding to the class token is fed to a Multi-Layer Perceptron (MLP) head for classification, and the outputs corresponding to the patch tokens are exposed. In this paper, we propose an improved ViT architecture (called ViT-Patch), which adds a shared MLP head to the output of each patch token to balance the feature learning on the class and patch tokens. In addition to the primary task, which uses the output of the class token to discriminate whether the image is malignant, a secondary task is introduced, which uses the output of each patch token to determine whether the patch overlaps with the tumor area. More interestingly, due to the correlation between the primary and secondary tasks, the supervisory information added to the patch tokens help with improving the performance of the primary task on the class token. The introduction of secondary supervision information also improves the attention interaction among the class and patch tokens. And by this way, ViT reduces the demand on dataset size. The proposed ViT-Patch is validated on a publicly available dataset, and the experimental results show its effectiveness for both malignant identification and tumor localization.
Simplified Optimization of the Magnetic Configuration of HEMP-Thrusters
Authors: Lewerentz Lars , Schneider Ralf
Published: March 2023

One type of ion thruster that has gained attention in recent years is the High Efficiency Multistage Plasma (HEMP) thruster. Optimizing the performance of these thrusters can be challenging due to the complexity of the underlying physics. Since the construction of new designs is expensive, cheaper methods for optimization, e.g., numerical optimization, are being sought. This paper presents a fast, analytical approach to finding realistic starting points for the magnetic geometry design of HEMP thrusters. First, a ratio of length to radius is presented, where the magnetic field is especially parallel at the center of the magnetic ring. This result is confirmed with the open-source library magpylib. Its speed and accuracy qualify this tool for further optimization processes. Here, we present some simple performance indicators, which may be beneficial to characterize the magnetic field structure for further optimization.
XRF Semi-Quantitative Analysis and Multivariate Statistics for the Classification of Obsidian Flows in the Mediterranean Area
Authors: Bonizzoni Letizia , Kulchytska Oleksandra , Ruschioni Giulia
Published: March 2023

Obsidian is a natural volcanic glass formed after eruptions if very rapid cooling of lava occurs. In particular conditions, the lava silicate ions cannot reach the crystalline lattice ordered formation and assume a chaotic arrangement, giving origin to obsidian flows. Obsidian has been used since the Paleolithic period to make tools because of its durability; in the Neolithic period, its trade played an important role in the Mediterranean area, and currently, obsidian is of particular interest for tracing prehistoric trading patterns. In this work, we present a semi-quantitative approach, exploiting energy-dispersive X-ray fluorescence (ED-XRF) coupled with principal component analysis. We consider geological samples from the five main collection sites of archaeological interest in the Mediterranean Basin (i.e., Pantelleria, Lipari, Palmarola, and Sardinia islands in Italy and Milos Island in Greece) and obtain a reliable classification of the fragments’ provenance, also comparing chemical fingerprints with data from the literature. Reported results show that this non-invasive semi-quantitative protocol could ease the application to archaeological samples, such as blades and splinters, permitting the classification of artifacts found in the archeological sites of the Mediterranean area even when relatively few samples are considered.
Experimental Research of the Initial Temperature and Additives Effect on the Ignition and Combustion Mechanisms of Composite Liquid Fuel in a High-Temperature Oxidizer
Authors: Glushkov Dmitrii , Klepikov Dmitrii , Nigay Aleksandr , Paushkina Kristina , Pleshko Andrei
Published: March 2023

Composite fuel is a promising energy source that allows for solving the problems of waste disposal with energy generation. Such fuel is the most accessible fuel and is cheap in comparison with fossil fuels widely used in industrial thermal power engineering. This paper presents the results of experimental studies on the effect of the initial temperature and the addition of combustible liquids and solid components on the ignition characteristics of composite fuel single droplets. Composite liquid fuels were prepared using the main components: bituminous coal, coal processing waste (filter cake), rapeseed oil, turbine oil, and water. The research was carried out for fuel droplets with an initial temperature from −60 to +60 and an ambient temperature from 700 to 1000 °C. The differences in the ignition delay times at conditions close to the limiting ones were 2–3.5 times. A promising direction for intensifying the processes of the ignition and combustion of composite liquid fuels under relatively intense heating is self-grinding into a large number of small fragments up to complete disintegration due to the dispersion effect. It has been experimentally found that the addition of highly flammable liquids (gasoline, kerosene, diesel fuel, formic acid) to the fuel composition in an amount of 5% is characterized by an intensification of ignition and burnout of droplets by about two times. The ignition delay time is reduced by 20–40%, while the size of the dispersion area is increased by 20–70%. The addition of formic acid to the composite fuel has a positive effect on the main ignition characteristics from 5 to 50%, and the addition of a similar amount of diesel fuel by 20–64%.
Pressure Sensitivity Prediction and Pressure Measurement of Fast Response Pressure-Sensitive Paint Based on Artificial Neural Network
Authors: Liao Xianhui , Wei Chunhua , Zuo Chenglin , Gao Zhisheng , Jiang Hailin , Liang Lei , Li Zhaoyan
Published: March 2023

The characterization of pressure-sensitive paint (PSP) is affected by many physical and chemical factors, making it is difficult to analyze the relationship between characterization and influencing factors. An artificial neural network (ANN)-based method for predicting pressure sensitivity using paint thickness and roughness was proposed in this paper. The mean absolute percentage error (MAPE) for predicting pressure sensitivity is 6.5088%. The difference of paint thickness and roughness between sample and model surface may be a source of experimental error in PSP pressure measurement tests. The Stern-Volmer coefficients A and B are strongly linked. Pressure sensitivity is approximately equal to coefficient B, so coefficient A is predicted using pressure sensitivity based on the same ANN, and the MAPE of predicting A is 2.1315%. Then, we try to calculate the pressure by using the thickness and roughness on a model to predict pressure sensitivity and Stern-Volmer coefficient A. The PSP pressure measurement test was carried out at the China Aerodynamic Research and Development Center. Using the Stern-Volmer coefficient calculated by the in situ method, the method in this paper, and the sample calibration experiment, the root mean square errors (RMSE) of the pressure are 47.4431 Pa, 63.4736 Pa, and 73.0223 Pa, respectively.
EffShuffNet: An Efficient Neural Architecture for Adopting a Multi-Model
Authors: Kim Jong-In , Yu Gwang-Hyun , Lee Jin , Vu Dang Thanh , Kim Jung-Hyun , Park Hyun-Sun , Kim Jin-Young , Hong Sung-Hoon
Published: March 2023

This work discusses the challenges of multi-label image classification and presents a novel Efficient Shuffle Net (EffShuffNet) based on a convolutional neural network (CNN) architecture to address these challenges. Multi-label classification is difficult as the complexity of prediction increases with the number of labels and classes, and current multi-model approaches require optimized deep learning models which increase computational costs. The EffShuff block divides the input feature map into two parts and processes them differently, with one half undergoing a lightweight convolution and the other half undergoing average pooling. The EffShuff transition component shuffles the feature maps after lightweight convolution, resulting in a 57.9% reduction in computational cost compared to ShuffleNetv2. Furthermore, we propose EffShuff-Dense architecture, which incorporates Dense connection to further emphasize low-level features. In experiments, the EffShuffNet achieved 96.975% accuracy in age and gender classification, which is 5.83% higher than the state-of-the-art, while EffShuffDenseNet was even better with 97.63% accuracy. Additionally, the proposed models were found to have better classification performance with smaller model sizes in fine-grained image classification experiments.