bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of synchronous irrigation on cyclic fatigue of nickel-titanium instrument in the dynamic and static models
Authors: Liu Haiyun , Li Yanfeng , Chai Guangquan , Lv Yuan , Li Changjian , Li Shumao , Yang Chunhao , Li Aishunag , Wang Yingdi , Li Weiwei
Published: January 2021

Objective:

To evaluate the effect of synchronous water irrigation on the fatigue resistance of nickel-titanium instrument.

Methods:

A standardized cyclic fatigue test models were established, and five types of nickel-titanium instruments (PTU F1, WO, WOG, RE, and M3) were applied. Each instrument was randomly divided into two groups ( N = 12). There was synchronous water irrigation in the experimental group, and no water irrigation in the control group. Besides, ProTaper Universal F1 was randomly divided into 10 groups ( N = 20). In the static group, nickel-titanium instruments were divided into one control group (no irrigation, N = 20) and six experimental group (irrigation, N = 20) based on different flow rate, angle and position; while in the dynamic group, instruments were divided into one control group (no irrigation, N = 20) and two experimental group (irrigation, N = 20) based on different flow rate. The rotation time (Time to Failure, TtF) of instruments was recorded and analyzed.

Results:

According to the static experiments, the TtF of instruments in all experimental groups was significantly higher than that in the static control group. Besides, the dynamic tests of PTU F1 showed that the TtF in the experimental group was significantly higher than that in the dynamic control group. Compared with control group, the TtF in the experimental groups increased by at least about 30% and up to 160%. The static and dynamic tests of PTU F1 showed that the TtF of nickel-titanium instrument in all experimental groups was significantly higher than that in the control group. However, there was no significant difference between any two experimental groups.

Conclusion:

Regardless of dynamic or static model, TtF with irrigation was longer than that with non-irrigation, indicating that synchronous irrigation can increase the fatigue resistance of nickel-titanium instrument. However, different irrigation conditions may have the same effect on the fatigue resistance.
Minimally invasive hysterectomy for endometriosis: Surgical outcomes based on surgeon specialty
Authors: Mutter Olga , Ackroyd Sarah A , Taylor George A , Diaz Juan
Published: January 2021

Introduction:

To evaluate differences in surgical outcomes of minimally invasive hysterectomy performed for endometriosis between general gynecologists and gynecologic oncologists.

Methods:

Utilizing the 2016–2018 American College of Surgeons National Surgical Quality Improvement Program (NSQIP) hysterectomy dataset, we evaluated baseline characteristics and surgical outcomes for patients who underwent a minimally invasive hysterectomy for endometriosis between general gynecology and gynecologic oncology groups.

Results:

From 2016 to 2018, a total of 3751 minimally invasive hysterectomies were performed for the primary diagnosis of endometriosis. Of these cases, 3129 (83.4%) were performed by general gynecologists and 622 (16.6%) by gynecologic oncologists. There were several differences in baseline characteristics between the groups. Notably, general gynecologists performed a higher proportion of vaginal hysterectomies (7.9% vs 0.6%, p < 0.01). There were no statistically significant differences in overall 30-day complications or mortality between general gynecology and oncology groups, with the exception of a higher rate of postoperative sepsis (0.8% vs 0.2%, p = 0.01) in hysterectomies performed by oncologists. Compared to general gynecologists, oncologists had a longer operative time (134.9 ± 65.4 min vs 129 ± 60.9 min, p = 0.05). Multivariate regression of multiple tracked and composite outcomes revealed no consistent confounding variables other than race. In fact, African American race was a statistically significant predictive factor of composite complications (OR 1.80, p < 0.01), morbidity (OR 1.84, p < 0.05), and unplanned readmission (OR 2.30, p < 0.01). Surgeon specialty was not associated with composite complications, hysterectomy-specific complications, or readmission.

Conclusion:

There are no significant differences in surgical outcomes for minimally invasive hysterectomy for endometriosis between these two surgical subspecialties.
A comparison of six outcome measures across the recovery period after distal radius fixation—Which to use and when?
Authors: Fang Christian , Fang Evan , Yee Dennis KH , Kwan Kenny , Leung Gladys , Leung Frankie
Published: January 2021

Purpose:

Many standardized outcome measures exist to measure recovery after surgical fixation of distal radius fractures, however, choosing the optimal instrument is difficult. We evaluated responsiveness, ceiling/floor effects, and criterion validity over multiple time intervals across a 2-year follow-up period for six commonly used instruments.

Methods:

A total of 259 patients who received open reduction and internal fixation for distal radius fractures between 2012 and 2015 were recruited. Patients were administered the Patient-Rated Wrist Evaluation ( PRWE), Shortened Disabilities of the Arm, Shoulder and Hand questionnaire ( QuickDASH), Green and O’Brien score (Cooney modification) ( CGNO), Gartland and Werley score (Sarmiento modification) ( SGNW), flexion-extension arc ( FEArc), and grip fraction test ( GripFrac) at 1.5, 3, 6, 12, and 24 months postoperatively. Responsiveness was evaluated by calculating standardized response means (SRM) and Cohen’s d effect sizes (ES), and by correlating each instrument’s change scores against those of QuickDASH and PRWE, which were also used as external comparators to assess criterion validity. Ceiling/floor effects were calculated for all measures at each time point.

Results:

SRM (1.5–24 months) were 1.81, 1.77, 1.43, 1.16, 2.23, 2.45 and ES (1.5–24 months) were 1.81, 1.82, 1.95, 1.31, 1.99 and 2.90 for QuickDASH, PRWE, CGNO, SGNW, FEArc, and GripFrac respectively. Spearman correlation coefficients against QuickDASH at 24 months were: 0.809, 0.248, 0.563, 0.285, and 0.318 for PRWE, CGNO, SGNW, FEArc, and GripFrac respectively. Significant (>15% of patients reaching maximum score) ceiling effects were observed before 6 months for PRWE and SGNW.

Conclusions:

Our evidence supports the use of QuickDASH, PRWE, FEArc and GripFrac up to 6 months postsurgery, and QuickDASH and PRWE after 6 months.

Level of evidence:

Level II.
Safe Femoral Fixation Depth and Orientation for Lateral Extra-Articular Tenodesis in Anterior Cruciate Ligament Reconstruction
Authors: Zhu Meng , Han Lee Dave Yee , Williams Andy
Published: January 2021

Background:

Patients who undergo anterior cruciate ligament (ACL) reconstruction (ACLR) can have a persistent postoperative pivot shift. Performing lateral extra-articular tenodesis (LET) concurrently has been proposed to address this; however, LET femoral fixation may interfere with the ACLR femoral tunnel, which could damage the ACL graft or its fixation.

Purpose:

To evaluate the safe maximum implant or tunnel depth for a modified Lemaire LET when combined with ACLR anteromedial portal femoral tunnel drilling and to validate the safe LET drilling angles to avoid conflict with the ACLR femoral tunnel.

Study Design:

Descriptive laboratory study.

Methods:

Twelve fresh-frozen cadaveric knees were used. With each knee at 120° of flexion, an ACLR femoral tunnel in the anteromedial bundle position was created arthroscopically via the anteromedial portal using a 5-mm offset guide, a guide wire, and an 8-mm reamer, which was left in situ. A modified Lemaire LET was performed using a 1 cm-wide iliotibial band strip harvested with the distal attachment intact, to be fixed in the femur. The desired LET fixation point was identified with an external aperture 10 mm proximal and 5 mm posterior to the fibular collateral ligament’s femoral attachment, and a 2.4-mm guide wire was drilled, aiming at 0°, 10°, 20°, or 30° anteriorly in the axial plane and at 0°, 10°, or 20° proximally in the coronal plane (12 different drilling angle combinations). The relationship between the LET drilling guide wire and the ACLR femoral tunnel reamer was recorded for each combination. When a collision with the femoral tunnel was recorded, the LET wire depth was measured.

Results:

Collision with the ACLR femoral tunnel occurred at a mean LET wire depth of 23.6 mm (range, 15-33 mm). No correlation existed between LET wire depth and LET drilling orientation ( r = 0.066; P = .67). Drilling angle in the axial plane was significantly associated with the occurrence of tunnel conflict ( P < .001). However, no such association was detected when comparing the drilling angle in the coronal plane ( P = .267).

Conclusion:

Conflict of LET femoral fixation with the ACLR femoral tunnel using anteromedial portal drilling occurred at a mean depth of 23.6 mm but also at a depth as little as 15 mm, which is shorter than most implants. When longer implants or tunnels are used, the orientation should be directed at least 30° anteriorly in the axial plane to minimize the risk of tunnel conflict, bearing in mind the risk of joint violation.

Clinical Relevance:

This study provides important information for surgeons performing LET in combination with ACLR anteromedial portal femoral tunnel drilling regarding safe femoral implant or tunnel length and orientation.
Book review: Ajay Gudavarthy (Ed.), Secular Sectarianism: Limits of Subaltern Politics
Authors: Ghosh Avilasha
Published: January 2021

Ajay Gudavarthy (Ed.), Secular Sectarianism: Limits of Subaltern Politics. New Delhi: SAGE Publications, 2019. 280 pp., ₹1,095, ISBN: 9789353286774.
Book review: Omar Sadr, Negotiating Cultural Diversity in Afghanistan
Authors: Saxena Chayanika
Published: January 2021

Omar Sadr, Negotiating Cultural Diversity in Afghanistan. Oxon: Routledge, 2020, xiii+242 pp., US$112.11, ISBN: 978-1138371057
Monobloc Facial Advancement in the World of COVID-19 Testing: A New Potential Risk for Iatrogenic Injury
Authors: Qamar Fatima , Whalen Kristen S. , Abbassi Bahar , Rodriguez Luis F. , Jallo George I. , Rottgers S. Alex
Published: January 2021

Background: Monobloc advancement with distraction is a holistic approach to treat the sequelae of syndromic craniosynostosis. These osteotomies create a communication between the nasal cavity and anterior cranial fossa that must be surgically obliterated to limit the infectious risks. In light of mandatory COVID-19 testing protocols amidst the pandemic, we aim to identify the specific challenges in management and present our strategy to overcome them. Case presentation: We report 2 cases of syndromic craniosynostosis treated with monobloc distraction during the COVID-19 pandemic and highlight safety concerns and our approach to managing these patients by avoiding nasopharyngeal swabs that may potentially penetrate the cranial base and place the patient as risk for iatrogenic injury. Discussion/conclusion: In patients undergoing surgical procedures that create a cranial base defect, caution must be exercised when attempting to test for COVID-19 which may be a source of potential iatrogenic injury. We propose a new algorithm for COVID-19 screening/surveillance following frontofacial advancement. Patients must be screened via alternative testing methods or treated as potential asymptomatic carriers of COVID-19 until postoperative imaging demonstrates complete ossification of the cranial base.
Dobrovolskyite, Na4Ca(SO4)3, a new fumarolic sulfate from the Great Tolbachik fissure eruption, Kamchatka Peninsula, Russia
Authors: Shablinskii Andrey P. , Filatov Stanislav K. , Krivovichev Sergey V. , Vergasova Lidiya P. , Moskaleva Svetlana V. , Avdontseva Eugeniya Yu. , Knyazev Alexander V. , Bubnova Rimma S.
Published: January 2021

Dobrovolskyite, Na4Ca(SO4)3, is a new sulfate mineral from the Great Tolbachik fissure eruption, Kamchatka peninsula, Russia. It occurs as aggregates of tabular crystals up to 1–2 mm in maximum dimension, with abundant gas inclusions. The empirical formula calculated on the basis of O = 12 is (Na3.90K0.10)Σ4(Ca0.45Mg0.16Cu0.12Na0.10)Σ0.83S3.08O12. The crystal structure of dobrovolskyite was determined using single-crystal X-ray diffraction data as: trigonal, R3, a = 15.7223(2), c = 22.0160(5) Å, V = 4713.1(2) Å3, Z = 18 and R1 = 0.072. The Mohs’ hardness is 3.5. The mineral is uniaxial (+), with ω = 1.489(2) and ɛ = 1.491(2) (λ = 589 nm). The seven strongest lines of the powder X-ray diffraction pattern [d, Å (I, %)(hkl)] are: 11.58(40)(101); 5.79(22)(202); 4.54(18)(030); 3.86(88)(033); 3.67(32)(006); 2.855(50)(306); and 2.682(100)(330). The mineral is named in honour of Prof. Dr. Vladimir Vitalievich Dolivo-Dobrovolsky (1927–2009), one of the leading Russian scientists in the field of petrology, crystal optics and crystal chemistry. The crystal structure of dobrovolskyite can be described as composed of three symmetrically independent rods running parallel to the c axis. The rods consist of six octahedral–tetrahedral [Na(SO4)6]11– or [Ca(SO4)6]10– clusters of central octahedra sharing common corners with six adjacent SO4 tetrahedra. Alternatively, the crystal structure of the mineral can be described as a 12-layer ABACABACABAC eutactic array of Na+ and Ca2+ cations, and vacancies with disordered (SO4) tetrahedra in interstices. Dobrovolskyite and similar minerals probably formed upon cooling of a high-temperature phase with disordered cation and anion arrangements.
RhoG deficiency abrogates cytotoxicity of human lymphocytes and causes hemophagocytic lymphohistiocytosis
Authors: Kalinichenko Artem , Perinetti Casoni Giovanna , Dupré Loïc , Trotta Luca , Huemer Jakob , Galgano Donatella , German Yolla , Haladik Ben , Pazmandi Julia , Thian Marini , Yüce Petronczki Özlem , Chiang Samuel C. , Taskinen Mervi , Hekkala Anne , Kauppila Saila , Lindgren Outi , Tapiainen Terhi , Kraakman Michael J. , Vettenranta Kim , Lomakin Alexis J. , Saarela Janna , Seppänen Mikko R. J. , Bryceson Yenan T. , Boztug Kaan
Published: January 2021

Exocytosis of cytotoxic granules (CG) by lymphocytes is required for the elimination of infected and malignant cells. Impairments in this process underly a group of diseases with dramatic hyperferritinemic inflammation termed hemophagocytic lymphohistiocytosis (HLH). Although genetic and functional studies of HLH have identified proteins controlling distinct steps of CG exocytosis, the molecular mechanisms that spatiotemporally coordinate CG release remain partially elusive. We studied a patient exhibiting characteristic clinical features of HLH associated with markedly impaired cytotoxic T lymphocyte (CTL) and natural killer (NK) cell exocytosis functions, who beared biallelic deleterious mutations in the gene encoding the small GTPase RhoG. Experimental ablation of RHOG in a model cell line and primary CTLs from healthy individuals uncovered a hitherto unappreciated role of RhoG in retaining CGs in the vicinity of the plasma membrane (PM), a fundamental prerequisite for CG exocytotic release. We discovered that RhoG engages in a protein–protein interaction with Munc13-4, an exocytosis protein essential for CG fusion with the PM. We show that this interaction is critical for docking of Munc13-4+ CGs to the PM and subsequent membrane fusion and release of CG content. Thus, our study illuminates RhoG as a novel essential regulator of human lymphocyte cytotoxicity and provides the molecular pathomechanism behind the identified here and previously unreported genetically determined form of HLH.
LRPAP1 autoantibodies in mantle cell lymphoma are associated with superior outcome
Authors: Thurner Lorenz , Fadle Natalie , Bittenbring Jörg Thomas , Regitz Evi , Schuck Rita , Cetin Onur , Stuhr Ariane , Rixecker Torben , Murawski Niels , Poeschel Viola , Kaddu-Mulindwa Dominic , Preuss Klaus-Dieter , Stilgenbauer Stephan , Hermine Olivier , Kluin-Nelemans Hanneke C. , Hartmann Sylvia , Dreyling Martin , Pott Christiane , Bewarder Moritz , Hoster Eva
Published: January 2021

Low-density lipoprotein (LDL) receptor-related protein-associated protein 1 (LRPAP1) had been identified by B-cell receptor (BCR) expression cloning and subsequent protein array screening as a frequent and proliferation-inducing autoantigen of mantle cell lymphoma (MCL). Of interest, high-titered and light chain–restricted LRPAP1 autoantibodies were detected in 8 of 28 patients with MCL. In the present study, LRPAP1 autoantibodies in sera of patients treated within the Younger and Elderly trials of the European MCL Network were analyzed regarding frequency, association with disease characteristics, and prognostic impact. LRPAP1 autoantibodies were detected in 41 (13%) of 312 evaluable patients with MCL. These LRPAP1 autoantibodies belonged predominantly to the immunoglobulin G (IgG) class and were clonally light chain restricted (27 with κ light chains, 14 patients with λ light chains). Titers ranged between 1:400 and 1:3200. The presence of LRPAP1 autoantibodies was not significantly associated with any baseline clinical characteristic, however, it was associated with a superior 5-year probability for failure-free survival (FFS) of 70% (95% confidence interval [CI], 57% to 87%) vs 51% (95% CI, 44% to 58%), P = .0052; and for overall survival (OS) of 93% (95% CI, 85% to 100%) vs 68% (95% CI, 62% to 74%), P = .0142. LRPAP1-seropositive patients had a Mantle Cell Lymphoma International Prognostic Index–adjusted hazard ratio for FFS of 0.48 (95% CI 0.27-0.83, P = .0083) and for OS of 0.47 (95% CI 0.24-0.94, P = .032). LRPAP1 autoantibodies were frequently detected in a large cohort of MCL patients treated within prospective multicenter clinical trials. Our results suggest better outcomes for LRPAP1-autoantibody seropositive patients.
Possible mechanisms underlying the association between human T-cell leukemia virus type 1 (HTLV-1) and hypertension in elderly Japanese population
Authors: Shimizu Yuji , Arima Kazuhiko , Noguchi Yuko , Kawashiri Shin-Ya , Yamanashi Hirotomo , Tamai Mami , Nagata Yasuhiro , Maeda Takahiro
Published: January 2021

Background

Human T-cell leukemia virus type 1 (HTLV-1) activates inflammatory cascades by activating the NF-κB pathway. The minor allele of single nucleotide polymorphism (SNP) in breast cancer suppressor BRCA1-associated protein (BRAP), which has a common etiology with HTLV-1 infection, has been reported to be positively associated with carotid atherosclerosis, but inversely associated with hypertension. Therefore, HTLV-1 infection may be inversely associated with hypertension by activating endothelial maintenance, including atherosclerosis. To clarify these associations, a cross-sectional study was conducted using 2989 Japanese individuals aged 60–99 years participating in a general health check-up.

Methods

Logistic regression models were used to clarify the association between HTLV-1 and hypertension. Platelet levels stratified analyses were also performed since platelet production, which plays a crucial role in endothelium maintenance, can be stimulated by activating the NF-κB pathway.

Results

HTLV-1 infection was found to be significantly inversely associated with hypertension, particularly in subjects with high platelet levels (≥ second tertiles of platelet levels); the fully adjusted odds ratios (ORs) and 95% confidence intervals (CIs) were 0.75 (0.62, 0.92) for total and 0.64 (0.50, 0.82) for high platelet levels, respectively. Further analysis of the non-hypertensive subjects demonstrated that HTLV-1 infection was significantly positively associated with atherosclerosis in subjects with the highest tertile of platelet levels (2.11 [1.15, 3.86]) but not in subjects with low platelet levels (first and second tertiles of platelet level) (0.89 [0.57, 1.39]).

Conclusion

Asymptomatic HTLV-1 infection is inversely associated with hypertension, possibly by activating endothelial maintenance, including atherosclerosis progression.
Peripheral retinal arteriolar leakage in giant cell arteritis: a case report
Authors: Ahmad Meleha , Carey Andrew R. , Eberhart Charles G. , Siadati Sepideh , Henderson Amanda D.
Published: January 2021

A 76-year old African American female with a history of arteritic ischemic optic neuropathy (AION) secondary to biopsy-proven giant cell arteritis (GCA) presented with unilateral vision loss in her contralateral eye despite high-dose oral steroid treatment. Dilated fundus examination revealed three cotton wool spots. Fluorescein angiography showed slowed arteriolar filling with late staining of small peripheral arteries, consistent with small vessel arteritis. Laboratory tests for alternative vasculitides were negative. Review of her temporal artery biopsy specimen confirmed lymphoplasmacytic inflammation around small adventitial vessels with no destructive granulomatous or leukocytoclastic small vessel vasculitis, consistent with GCA. Our unique case demonstrates peripheral small vessel retinal arteriolar leakage in GCA, which is a rare finding. This association is of interest because GCA is commonly associated with medium to large vessel pathology without small vessel involvement.
Data-driven biological network alignment that uses topological, sequence, and functional information
Authors: Gu Shawn , Milenković Tijana
Published: January 2021

Background

Network alignment (NA) can transfer functional knowledge between species’ conserved biological network regions. Traditional NA assumes that it is topological similarity (isomorphic-like matching) between network regions that corresponds to the regions’ functional relatedness. However, we recently found that functionally unrelated proteins are as topologically similar as functionally related proteins. So, we redefined NA as a data-driven method called TARA, which learns from network and protein functional data what kind of topologicalrelatedness(rather than similarity) between proteins corresponds to their functional relatedness. TARA used topological information (within each network) but not sequence information (between proteins across networks). Yet, TARA yielded higher protein functional prediction accuracy than existing NA methods, even those that used both topological and sequence information.

Results

Here, we propose TARA++ that is also data-driven, like TARA and unlike other existing methods, but that uses across-network sequence information on top of within-network topological information, unlike TARA. To deal with the within-and-across-network analysis, we adapt social network embedding to the problem of biological NA. TARA++ outperforms protein functional prediction accuracy of existing methods.

Conclusions

As such, combining research knowledge from different domains is promising. Overall, improvements in protein functional prediction have biomedical implications, for example allowing researchers to better understand how cancer progresses or how humans age.
The effect of a multicomponent exercise protocol (VIVIFRAIL©) on inflammatory profile and physical performance of older adults with different frailty status: study protocol for a randomized controlled trial
Authors: Petrella Marina , Aprahamian Ivan , Mamoni Ronei Luciano , de Vasconcellos Romanini Carla Fernanda , Lima Natália Almeida , de Cássio Robello Everson , da Costa Daniele Lima , An Vinicius Nakajima , Aguirre Bianca Nobre , Galdeano Júlia Riccetto , Fernandes Isabela Cunha , Soleman Hernandez Salma S. , Cesari Matteo , Morley John E. , Izquierdo Mikel , Oude Voshaar Richard C.
Published: January 2021

Background

To investigate whether an exercise intervention using the VIVIFRAIL© protocol has benefits for inflammatory and functional parameters in different frailty status.

Methods/design

This is a randomized clinical trial in an outpatient geriatrics clinic including older adults ≥60 years. For each frailty state (frail, pre-frail and robust), forty-four volunteers will be randomly allocated to the control group (n = 22) and the intervention group (n = 22) for 12 weeks. In the control group, participants will have meetings of health education while those in the intervention group will be part of a multicomponent exercise program (VIVIFRAIL©) performed five times a week (two times supervised and 3 times of home-based exercises). The primary outcome is a change in the inflammatory profile (a reduction in inflammatory interleukins [IL-6, TNF- α, IL1beta, IL-17, IL-22, CXCL-8, and IL-27] or an increase in anti-inflammatory mediators [IL-10, IL1RA, IL-4]). Secondary outcomes are change in physical performance using the Short Physical Performance Battery, handgrip strength, fatigue, gait speed, dual-task gait speed, depressive symptoms, FRAIL-BR and SARC-F scores, and quality of life at the 12-week period of intervention and after 3 months of follow-up.

Discussion

We expect a reduction in inflammatory interleukins or an increase in anti-inflammatory mediators in those who performed the VIVIFRAIL© protocol. The results of the study will imply in a better knowledge about the effect of a low-cost intervention that could be easily replicated in outpatient care for the prevention and treatment of frailty, especially regarding the inflammatory and anti-inflammatory pathways involved in its pathophysiology.

Trial registration

Brazilian Registry of Clinical Trials (RBR-9n5jbw; 01/24/2020). Registred January 2020. http://www.ensaiosclinicos.gov.br/rg/RBR-9n5jbw/.
Immunofluorescence deposits in the mesangial area and glomerular capillary loops did not affect the prognosis of immunoglobulin a nephropathy except C1q:a single-center retrospective study
Authors: Wu Lingzhi , Liu Di , Xia Ming , Chen Guochun , Liu Yu , Zhu Xuejing , Liu Hong
Published: January 2021

Background

Immunoglobulin A nephropathy (IgAN) is identified as mesangial IgA deposition and is usually accompanied by other immunofluorescence deposits. The impact of immunofluorescent features in IgAN patients, however, remains unclear.

Methods

Baseline clinicopathologic parameters and renal outcomes of 337 patients diagnosed with IgAN between January 2009 and December 2015 were analyzed. We then categorized these patients into four groups: without immunofluorescence deposits, mesangial-only, mesangial and glomerular capillary loops (GCLs), and GCLs-only. The study endpoint was end-stage kidney disease (ESKD) or a ≥ 50% decline in the estimated glomerular filtration rate (eGFR). Kaplan–Meier and Cox regression analyses were performed to calculate renal survival.

Results

Of the 337 IgAN patients, women comprised 57.0%. Compared to patients with IgA deposition in the mesangial-only group, patients with IgA deposition in the mesangial +GCLs group were much heavier, and exhibited higher systolic blood pressure, lower serum IgG levels, and heavier proteinuria (all P < 0.05). Patients with IgG deposition in the mesangial +GCLs group presented with higher levels of cholesterol, heavier proteinuria than IgG deposition in the mesangial-only group (both P < 0.05). Compared with the mesangial-only group exhibiting C3 deposits, patients in the mesangial +GCLs group with C3 deposition had a higher systolic blood pressure (P = 0.028). A total of 38 patients (11.3%) continued to the study endpoint after a median follow-up time of 63.5 months (range,49.8–81.4). Kaplan–Meier analysis and Cox regression analysis showed that C1q deposition in the mesangial +GCLs group predicted a poor renal prognosis.

Conclusions

IgA and IgG deposits in the mesangial region and GCLs were associated with more unfavorable clinical and histopathologic findings in IgAN patients. C1q deposition in the mesangial region and GCLs predicted a poor renal prognosis. However, the impact of the pattern of immunofluorescence deposits on renal outcomes remains to be proven by further investigation.
LncRNA WT1-AS downregulates lncRNA UCA1 to suppress non-small cell lung cancer and predicts poor survival
Authors: Wan Yanhui , Yao Da , Fang Fuyuan , Wang Youyu , Wu Guodong , Qian Youhui
Published: January 2021

Background

LncRNA WT1-AS inhibits gastric cancer, while its role in other cancers is unknown. We investigated the role of WT1-AS in non-small cell lung cancer (NSCLC).

Methods

Sixty-six NSCLC patients (40 males and 26 females; 36 to 68 years old; mean age 52.7 ± 6.4 years old) were selected from the 178 NSCLC patients operated on for lung cancer between 2010 and 2013. RT-qPCR was used to analyze the expression of lncRNA. Overexpression experiments were performed to assess interactions between lncRNAs. CCK-8 assay was carried to evaluate the roles of WT1-AS and UCA1 in regulating cell proliferation. Cell invasion and migration assays were performed to assess the roles of WT1-AS and UCA1 in regulating cell invasion and migration. Western-blot was performed to illustrate the effect of WT1-AS and UCA1 in EMT.

Results

WT1-AS was downregulated in NSCLC and was correlated with poor survival. The expression of WT1-AS in NSCLC was not correlated with clinical stages. LncRNA UCA1 was upregulated in cancer tissues and inversely correlated with WT1-AS. Overexpression of UCA1 did not affect WT1-AS, while overexpression of WT1-AS led to inhibited expression of UCA1. Overexpression of UCA1 resulted in increased proliferation, EMT, migration and invasion of NSCLC cells, while overexpression of WT1-AS showed opposite effects. In addition, overexpression of UCA1 inhibited the role of overexpression of WT1-AS.

Conclusions

Therefore, overexpression of WT1-AS may inhibit the cell proliferation and EMT to decrease cell migration and invasion of NSCLC cells by downregulating UCA1.
Correction to: Tumor vasculature-targeted 10B delivery by an Annexin A1-binding peptide boosts effects of boron neutron capture therapy
Authors: Yoneyama Tohru , Hatakeyama Shingo , Sutoh Yoneyama Mihoko , Yoshiya Taku , Uemura Tsuyoshi , Ishizu Takehiro , Suzuki Minoru , Hachinohe Shingo , Ishiyama Shintaro , Nonaka Motohiro , Fukuda Michiko N. , Ohyama Chikara
Published: January 2021

An amendment to this paper has been published and can be accessed via the original article.
Correction to: MicroRNA-99a induces G1-phase cell cycle arrest and suppresses tumorigenicity in renal cell carcinoma
Authors: Cui Li , Zhou Hua , Zhao Hu , Zhou Yaojun , Xu Renfang , Xu Xianlin , Zheng Lu , Xue Zhong , Xia Wei , Zhang Bo , Ding Tao , Cao Yunjie , Tian Zinong , Shi Qianqian , He Xiaozhou
Published: January 2021

An amendment to this paper has been published and can be accessed via the original article.
Identifying opportunities to strengthen school food environments in the Pacific: a case study in Samoa
Authors: Reeve Erica , Thow Anne-Marie , Bell Colin , Soti-Ulberg Christina , Sacks Gary
Published: January 2021

Background

Despite global recommendations to prioritise policies that create healthy food environments within education institutions, the implementation of effective healthy school food policies has proved challenging for many countries. This study examined the experience of Samoa subsequent to the 2012 introduction of a stronger policy to improve the healthiness of school food environments. Our aim was to identify opportunities to strengthen healthy school food policy implementation in Samoa and other comparable contexts.

Methods

We used a qualitative case study approach, underpinned by policy science theory. In 2018, we conducted in-depth semi-structured interviews with 30 informants, coupled with analysis of relevant documents, to generate a detailed understanding of the relevant policy implementation processes in Samoa, and the perspectives and capacities of key implementation actors. Data collection and analysis were guided by the Health Policy Analysis Triangle, supplemented by other policy theories relevant to policy process.

Results

Samoa’s school food policy operationalizes international ‘best practice’ recommendations. We found health policymakers and leaders in Samoa to be strongly committed to improving school food environments. Despite this, there continued to be challenges in ensuring compliance with the school nutrition standards. Key issues that negatively impacted the policy’s effectiveness were the lack of priority given to school food by stakeholders outside of health, the high prevalence of unhealthy food in the areas immediately surrounding schools, vendor knowledge and capacity, and the high degree of agency exercised by actors in and around the school. We noted several opportunities for policies to be effectively implemented and sustained. Respondents identified community-level leaders as potentially pivotal stakeholders, particularly where school governance arrangements draw heavily on community representation.

Conclusions

Sustained and effective implementation of healthy school food policies requires continued engagement from political and community leaders, beyond initial commitment. There is potential to capitalize on political will for diet-related NCD prevention by more clearly demonstrating the institutional and operational requirements for effective and sustained implementation. Strong incentives for compliance and effective enforcement mechanisms are also likely to be crucial to success.
Distribution of risk factors of hypertension patients in different age groups in Tianjin
Authors: Zhang Yingyi , Yang Hua , Ren Min , Wang Ruiying , Zhao Fumei , Liu Ting , Zhang Ying , Guo Zhigang , Cong Hongliang
Published: January 2021

Background

To analyze the risk factors for hypertension in different age groups of urban and rural residents in Tianjin.

Methods

A total of 33,997 people (35–75 years old) from 13 community health service centers and primary hospitals in Tianjin participated in this study. They were divided into the youth group (≤ 40 years old), middle-aged group (41–65 years old), and elderly group (> 65 years old). Then, a questionnaire survey was administered, followed by physical and blood biochemical examinations. The demographic characteristics and prevalence were recorded and counted. Subsequently, risk factors were analyzed using univariate and stepwise multivariate logistic regression analysis.

Results

In the youth, middle-aged, and elderly groups, the prevalence rate of hypertension was 18.65, 51.80, and 76.61%, respectively. Logistic regression analysis showed that obesity(OR: 3.263, 95% CI: 1.039–1.656), men (OR: 2.117, 95% CI: 1.691–2.651), diabetes (OR: 1.978, 95% CI: 1.398–2.799), high triglycerides(OR 1.968 95% CI: 1.590–2.434) and family history of stroke (OR: 1.936, 95% CI: 1.287–2.911) are the five factors in youth. In middle-aged group, the significantly associating factors were obesity (OR: 2.478, 95% CI: 2.330–2.636), diabetes (OR: 2.173, 95% CI: 1.398–2.799), family history of stroke (OR: 1.808, 95% CI: 1.619–2.020), maleness (OR: 1.507, 95% CI: 1.412–1.609),Hypertriglyceridemia (OR 1.490 95% CI: 1.409–1.577),family history of cardiovascular disease (OR: 1.484, 95% CI: 1.307–1.684),Hypercholesterolemia (OR 1.228 95% CI: 1.160–1.299). In the elderly group, obesity (OR: 2.104, 95% CI: 1.830–2.418), family history of strokes (OR: 1.688, 95% CI: 1.243–2.292), diabetes mellitus (OR: 1.544, 95% CI: 1.345–1.773), family history of cardiovascular disease (OR: 1.470, 95% CI: 1.061–2.036), hypertriglyceridemia (OR: 1.348, 95% CI: 1.192–1.524) increased the risk for hypertension. Waist circumference (WC) and waist-to-height ratio (WHtR) increased with age, and the value of these two measures for predicting hypertension was better than BMI in middle-aged group.

Conclusion

Obesity is the most important risk factor for hypertension in all age groups. Diabetes, family history of strokes and high triglyceride were also significant risk factors for all age groups. There was a gender difference between the young and middle-aged groups, with men more likely to hypertension. Waist circumference (WC) and waist-to-height ratio (WHtR) were better predictors of hypertension than BMI in middle-aged group.
Accuracy measures of 1.5-tesla MRI for the diagnosis of ACL, meniscus and articular knee cartilage damage and characteristics of false negative lesions: a level III prognostic study
Authors: Koch Jonathan E. J. , Ben-Elyahu Ron , Khateeb Basel , Ringart Michael , Nyska Meir , Ohana Nissim , Mann Gideon , Hetsroni Iftach
Published: January 2021

Background

MRI is the most accurate imaging modality for diagnosing knee pathologies. However, there is uncertainty concerning factors predicting false negative MRI, such as meniscal tear patterns as well as patient factors. The aims of this study were to report 1.5-Tesla MRI accuracy of ACL, meniscus and articular cartilage damage and characterize false negative lesions.

Methods

Two hundred eighteen consecutive knee arthroscopies performed in our institution between 2013 and 2016 and their respective prospectively-collected MRI reports were reviewed. Inclusion criteria were age > 15 years-old, primary arthroscopy, 1.5-Tesla MRI performed at the same institution, and time interval MRI-surgery < 6 months. Exclusion criteria were revision arthroscopy and arthroscopic-assisted fracture fixation or multiligament surgery. Accuracy measures and Kappa coefficients were calculated comparing the MRI diagnosis to the arthroscopic findings. Moreover, the arthroscopic findings of false negative MRI were compared to the findings of true positive MRI using the Fisher-exact test. Pearson correlation was used for testing the correlation between MRI accuracy and patient age.

Results

The highest accuracy was observed in medial meniscus and in ACL findings. For the medial meniscus sensitivity, specificity, agreement, and Kappa coefficient were 77, 92, 86%, and 0.7, and for the ACL these measures were 82, 97, 87%, and 0.73. MRI accuracy was lower in the lateral meniscus and articular cartilage with Kappa coefficient 0.42 and 0.3, respectively. More specifically, short peripheral tears in the posterior horn of the medial meniscus were characteristic of false negative findings compared to true positive findings of the MRI (p <  0.01). MRI accuracy correlated negatively compared to arthroscopic findings with patient age for the medial meniscus (r = − 0.21, p = 0.002) and for articular cartilage damage (r = − 0.45, p <  0.001).

Conclusion

1.5-Tesla MRI will accurately diagnose ACL and medial meniscal tears and can reliably complete the diagnostic workup following physical examination, particularly in young adults. This modality however is not reliable for diagnosing short peripheral tears at the posterior horn of the medial meniscus and partial thickness articular cartilage lesion of the femoral condyles. For these lesions, definitive diagnosis may require cartilage-specific MRI sequences or direct arthroscopic evaluation.

Level of evidence

Prognostic study, Level III.
Using text analysis software to identify determinants of inappropriate clinical question reporting and diagnostic procedure referrals in Reggio Emilia, Italy
Authors: Venturelli Francesco , Ottone Marta , Pignatti Fabio , Bellocchio Eletta , Pinotti Mirco , Besutti Giulia , Djuric Olivera , Giorgi Rossi Paolo
Published: January 2021

Background

Inappropriate prescribing of diagnostic procedures leads to overdiagnosis, overtreatment and resource waste in healthcare systems. Effective strategies to measure and to overcome inappropriateness are essential to increasing the value and sustainability of care.

We aimed to describe the determinants of inappropriate reporting of the clinical question and of inappropriate imaging and endoscopy referrals through an analysis of general practitioners’ (GP) referral forms in the province of Reggio Emilia, Italy.

Methods

A clinical audit was conducted on routinely collected referral forms of all GPs of Reggio Emilia province. All prescriptions for gastroscopy, colonoscopy, neurological and musculoskeletal computerised tomography (CT) and magnetic resonance imaging (MRI) from 2012 to 2017 were included. The appropriateness of referral forms was assessed using Clinika VAP software, which combines semantic analysis of clinical questions and available metadata. Local protocols agreed on by all physicians defined criteria of appropriateness. Two multilevel logistic models were used to identify multiple predictors of inappropriateness of referral forms and to analyse variability among GPs, primary care subdistricts and healthcare districts.

Results

Overall, 37% of referral forms were classified as inappropriate, gastroscopy and CT showed higher proportions of inappropriate referrals compared to colonoscopy and MRI. Inappropriateness increased with patient age for CT and MRI; for gastroscopy, it was lower for patients aged 65–84 compared to those younger, and for colonoscopy, it was higher for older patients. Fee exemptions were associated with inappropriateness in MRI referral forms. The effect of GPs’ practice organization was consistent across all tests, showing higher inappropriateness for primary care medical networks than in primary care medical groups. Male GPs were associated with inappropriateness in endoscopy, and older GPs were associated with inappropriateness in musculoskeletal CT. While there was moderate variability in the inappropriate prescribing among GPs, there was not among the healthcare districts or primary care subdistricts.

Conclusions

Routinely collected data and IT tools can be useful to identify and monitor diagnostic procedures at high risk of inappropriate prescribing. Assessing determinants of inappropriate referral makes it possible to tailor educational and organizational interventions to those who need them.
XenoCell: classification of cellular barcodes in single cell experiments from xenograft samples
Authors: Cheloni Stefano , Hillje Roman , Luzi Lucilla , Pelicci Pier Giuseppe , Gatti Elena
Published: January 2021

Background

Single-cell sequencing technologies provide unprecedented opportunities to deconvolve the genomic, transcriptomic or epigenomic heterogeneity of complex biological systems. Its application in samples from xenografts of patient-derived biopsies (PDX), however, is limited by the presence of cells originating from both the host and the graft in the analysed samples; in fact, in the bioinformatics workflows it is still a challenge discriminating between host and graft sequence reads obtained in a single-cell experiment.

Results

We have developed XenoCell, the first stand-alone pre-processing tool that performs fast and reliable classification of host and graft cellular barcodes from single-cell sequencing experiments. We show its application on a mixed species 50:50 cell line experiment from 10× Genomics platform, and on a publicly available PDX dataset obtained by Drop-Seq.

Conclusions

XenoCell accurately dissects sequence reads from any host and graft combination of species as well as from a broad range of single-cell experiments and platforms. It is open source and available at https://gitlab.com/XenoCell/XenoCell.
Mitotic arrest affects clustering of tumor cells
Authors: Bonnet Julia , Rigal Lise , Mondesert Odile , Morin Renaud , Corsaut Gaëlle , Vigneau Mathieu , Ducommun Bernard , Lobjois Valérie
Published: January 2021

Background

Cancer cell aggregation is a key process involved in the formation of tumor cell clusters. It has recently been shown that clusters of circulating tumor cells (CTCs) have an increased metastatic potential compared to isolated circulating tumor cells. Several widely used chemotherapeutic agents that target the cytoskeleton microtubules and cause cell cycle arrest at mitosis have been reported to modulate CTC number or the size of CTC clusters.

Results

In this study, we investigated in vitro the impact of mitotic arrest on the ability of breast tumor cells to form clusters. By using live imaging and quantitative image analysis, we found that MCF-7 cancer cell aggregation is compromised upon incubation with paclitaxel or vinorelbine, two chemotherapeutic drugs that target microtubules. In line with these results, we observed that MCF-7 breast cancer cells experimentally synchronized and blocked in metaphase aggregated poorly and formed loose clusters. To monitor clustering at the single-cell scale, we next developed and validated an in vitro assay based on live video-microscopy and custom-designed micro-devices. The study of cluster formation from MCF-7 cells that express the fluorescent marker LifeAct-mCherry using this new assay allowed showing that substrate anchorage-independent clustering of MCF-7 cells was associated with the formation of actin-dependent highly dynamic cell protrusions. Metaphase-synchronized and blocked cells did not display such protrusions, and formed very loose clusters that failed to compact.

Conclusions

Altogether, our results suggest that mitotic arrest induced by microtubule-targeting anticancer drugs prevents cancer cell clustering and therefore, could reduce the metastatic potential of circulating tumor cells.
Targeting B7-H3 via chimeric antigen receptor T cells and bispecific killer cell engagers augments antitumor response of cytotoxic lymphocytes
Authors: Liu Jie , Yang Shuo , Cao Bihui , Zhou Guangyu , Zhang Fengjuan , Wang Yuan , Wang Rixin , Zhu Lipeng , Meng Ya , Hu Cong , Liang Hui , Lin Xu , Zhu Kangshun , Chen Guokai , Luo Kathy Qian , Di Lijun , Zhao Qi
Published: January 2021

Background

B7-H3, an immune-checkpoint molecule and a transmembrane protein, is overexpressed in non-small cell lung cancer (NSCLC), making it an attractive therapeutic target. Here, we aimed to systematically evaluate the value of B7-H3 as a target in NSCLC via T cells expressing B7-H3-specific chimeric antigen receptors (CARs) and bispecific killer cell engager (BiKE)-redirected natural killer (NK) cells.

Methods

We generated B7-H3 CAR and B7-H3/CD16 BiKE derived from an anti-B7-H3 antibody omburtamab that has been shown to preferentially bind tumor tissues and has been safely used in humans in early-phase clinical trials. Antitumor efficacy and induced-immune response of CAR and BiKE were evaluated in vitro and in vivo. The effects of B7-H3 on aerobic glycolysis in NSCLC cells were further investigated.

Results

B7-H3 CAR-T cells effectively inhibited NSCLC tumorigenesis in vitro and in vivo. B7-H3 redirection promoted highly specific T-cell infiltration into tumors. Additionally, NK cell activity could be specially triggered by B7-H3/CD16 BiKE through direct CD16 signaling, resulting in significant increase in NK cell activation and target cell death. BiKE improved antitumor efficacy mediated by NK cells in vitro and in vivo, regardless of the cell surface target antigen density on tumor tissues. Furthermore, we found that anti-B7-H3 blockade might alter tumor glucose metabolism via the reactive oxygen species-mediated pathway.

Conclusions

Together, our results suggest that B7-H3 may serve as a target for NSCLC therapy and support the further development of two therapeutic agents in the preclinical and clinical studies.