bluuebunny/crossref_metadata_2025 huggingface dataset:
Regenerative Textiles: A Framework for Future Materials Circularity in the Textile Value Chain
Authors: Ribul Miriam
Published: December 2021

Materials science breakthroughs have regenerated high value fibres from end-of-life cellulose-based textiles that can be introduced into existing textile fabrication processes from raw material to textile product in established textile value chains. Scientific developments with regenerated cellulose fibres obtained from waste textiles suggest their potential to replace virgin resources. The current scale-up of regeneration technologies for end-of-life cellulose-based textiles towards pilot and commercial scales can potentially achieve a future materials circularity, but there is a lack of a long-term view of the properties of materials after consecutive recycling stages take place. Cellulose-based materials cannot be infinitely recycled and maintain the same quality, a factor which may provide new challenges for future textile processes in the context of the circular bioeconomy. This paper maps collaborative design and materials science projects that use regenerated cellulose obtained from waste feedstock according to materials in the value chain they seek to substitute. It also presents four new processes that use regenerated cellulose materials in relation to their intervention in the value chain (as determined in a PhD investigation). A framework is presented to demonstrate how these circular material design processes take place at earlier stages of the textile value chain after subsequent regeneration stages.
Influence Mechanism of Cyanobacterial Extracellular Polymeric Substances on the Water Quality in Dynamic Water Supply System
Authors: Sun Feng , Yu Peng , Xu Chenhui , Yu Hongfei , Wang Fengyi , Zhou Yang , Cong Haibing
Published: December 2021

As a kind of high-organic-content contamination source, extracellular polymeric substances (EPS) secreted by cyanobacteria have become an important factor restricting the safety of supply water. In the dynamic batch mode water supply system, cyanobacterial EPS accelerated the decay rate of residual chlorine, resulting in a 21–26 times increase of the total viable bacteria count within 72 h. The water turbidity exceeded upper limit of the standards for drinking water quality within 4 h, with an increase of 306–332% within 72 h. The biological stability was reduced with BDOC and AOC increased by 41.4–43.8% and 331–396%, respectively. The main cause is that cyanobacterial EPS act as nutrients and metabolic energy for microorganisms, promoting their metabolic activity and secretion of extracellular organic components. This leads to the metabolic accumulation of tryptophan, fulvic acids and humic acids in the pipeline, thus further promoting the regeneration of bacteria. Compared with the influence of biofilm on pipe wall caused by long-term use of water supply network, the contribution of cyanobacterial EPS to the water contamination of pipe network is increased several times. Therefore, even in the presence of residual chlorine, the secondary contamination caused by cyanobacterial EPS in the water supply system could not be neglected.
A Hypothetical Extraction Method Decomposition of Intersectoral and Interprovincial CO2 Emission Linkages of China’s Construction Industry
Authors: Ogungbile Adedayo Johnson , Shen Geoffrey Qiping , Xue Jin , Alabi Tobi Michael
Published: December 2021

Understanding the complex CO2 emissions in inter-sectoral and interregional interactions of the construction industry is significant to attaining sustainability in China. Many previous studies focused on aggregating the construction sector’s CO2 emissions on a national level, with the provincial characteristics and interactions often overlooked. Using extended environmental input–output tables, we adopted a hypothetical extraction method combined with extended-environmental multi-regional input–output tables for 2012, 2015, and 2017 data to decompose the CO2 emissions linkages in 30 provincial construction sectors. The provincial carbon emissions data from a complete system boundary informed the recategorization of China’s construction sector as a high-carbon-intensity industry. The interprovincial interactions results show relatively small backward CO2 emissions linkages compared to forward CO2 emissions linkages depicting the industry’s significant role in China’s economic growth and an essential target in CO2 emissions reduction plans. The provinces exhibited different impacts on the directional push–pull, with less developed provinces having one-way directional effects. The more developed provincial sectors behaved more like demand-driven industries creating an overall imbalance in CO2 emissions interaction between the sectors in interregional emission trades. We identified construction sectors in Gansu, Xingjian, Ningxia, and Inner Mongolia as the most critical, with more significant CO2 emissions interactions than other provinces. Improving the technical level in less developed provincial construction sectors, considering provincial characteristics in policy formulation, and a swift shift to renewable energy as a primary energy source would aid in reducing the emissions intensities in the construction sector, especially in the less developed provinces, and achieving China’s quest to reach a CO2 emissions peak by 2030.
Understanding the Heterogeneity of Human Mobility Patterns: User Characteristics and Modal Preferences
Authors: Wu Laiyun , Hasan Samiul , Chung Younshik , Kang Jee Eun
Published: December 2021

Characterizing individual mobility is critical to understand urban dynamics and to develop high-resolution mobility models. Previously, large-scale trajectory datasets have been used to characterize universal mobility patterns. However, due to the limitations of the underlying datasets, these studies could not investigate how mobility patterns differ over user characteristics among demographic groups. In this study, we analyzed a large-scale Automatic Fare Collection (AFC) dataset of the transit system of Seoul, South Korea and investigated how mobility patterns vary over user characteristics and modal preferences. We identified users’ commuting locations and estimated the statistical distributions required to characterize their spatio-temporal mobility patterns. Our findings show the heterogeneity of mobility patterns across demographic user groups. This result will significantly impact future mobility models based on trajectory datasets.
Culturally Responsive Teaching in Technology-Supported Learning Environments in Marine Education for Sustainable Development
Authors: Cheng Ming-Min , Lacaste Aurora V. , Saranza Cris , Chuang Hsueh-Hua
Published: December 2021

This study examined and evaluated how culturally responsive teaching in technology-supported learning environments for preservice teachers was practiced and modeled using experiential learning theory as a guiding framework. Results from qualitative analysis of observational data and outputs of 19 preservice teachers showed that the latter were able to include cultural values and harness technology in their teaching. It was also found that cultural scaffolding enhanced by technology is the most practiced culturally responsive teaching construct during teaching demonstrations. However, technology was used as teachers’ instructional tools—in the form of visual aids that illustrate abstract multicultural concepts—instead of students’ learning tools. Our findings could be used to develop a K-12 curriculum progression that provides a culturally responsive and contextualized teaching and learning environment for sustainable development.
Examining HRM Practices in Relation to the Retention and Commitment of Talented Employees
Authors: Tej Juraj , Vagaš Matúš , Ali Taha Viktória , Škerháková Veronika , Harničárová Michaela
Published: December 2021

The claim that human resources or human capital is the most important and valuable asset of organizations is widespread and widely accepted. For this reason, one of the biggest challenges for organizations is to find the right employees with the necessary skills, high potential, and talent. However, the challenge for organizations is not only to find, but also to retain and to use the potential of talented employees. The main aim of this study is to identify the HRM practices which most significantly affect the retention and commitment of talented employees. Based on factor analysis, individual factors of HRM practices were identified. Through canonical analysis, the relationships between the identified factors of HRM practices, talent retention (the intention of talented employees to remain in the organization), and the organizational commitment of talented employees were identified. One of the most important results of the study is the identification of human resource management practices that are most strongly associated with talent retention and commitment.
Influence of Stiffeners for Improving the Compressive Strength of Ventilated Corrugated Packages Using Finite Element Modelling Technique
Authors: Kalita Nilotpal , Saxena Prateek , Talha Mohammad
Published: December 2021

The aim of this research is to optimize the corrugated fibreboard (CFB) boxes recommended by the Bureau of Indian Standards (BIS) for packaging apples and suggest improvements for the same with the help of finite element modelling technique. The motivation for the current study grew with the advent of the multipurpose use of corrugated fibreboard as a structural material, particularly for packaging boxes. A standard double walled (DW) panel box is considered for improvements in terms of ventilation slot configuration and reduction in material utilization for manufacturing through the implementation of a single walled (SW) panel box design. In order to maintain the structural integrity upon reduction in box construction material, the concept of stiffener has been introduced as a load-bearing enhancement feature. These enhancements will help establish a rationale and enable the corrugated fibreboard manufacturers and consumers to improve the understanding of the behaviour of such structures and help to check for the cost utilization, avoid overdesign and further mitigate the failures in practical applications. Full depth vertical rectangular slot was found to generate the least stresses and found to be suitable as ventilation slots. With the replacement with SW panel box, a saving in material consumption can be realized amounting to more than 34%. It was found that a 3-ply box configuration with a full-depth ventilation slot with panel stiffener can serve as a potential candidate for the replacement of the current box constituting of 5-ply panels recommended in the context of Indian standards.
Digital Echelons and Interfaces within Value Chains: End-to-End Marketing and Logistics Integration
Authors: Barykin Sergey Evgenievich , Smirnova Elena Aleksandrovna , Chzhao Dan , Kapustina Irina Vasilievna , Sergeev Sergey Mikhailovich , Mikhalchevsky Yuri Yurievich , Gubenko Alexander Viktorovich , Kostin Gennady Aleksanrovich , De La Poza Plaza Elena , Saychenko Lilya , Moiseev Nikita
Published: December 2021

The goals of real business in the context of the digital transformation of international logistics networks and marketing channels have necessitated the application of a scientifically based theoretical approach to the development of a formalized description acceptable for predictive planning based on leading indicators. In the context of globalization and interstate and regional economic unions, this will lead to achieving the maximum end-to-end integration of digital platforms. Based on the analysis, the article presents the integration of digital logistics and marketing approaches with the mathematical models of the ecosystem organization of economic relations. The features of the organization of economic relations between contractors involved in the execution of virtual transactions and the material movement of resources were analyzed. The researchers considered prerequisites for the analytical description of interconnections between the participants of digital platforms in cross border e-commerce. The authors’ approach is based on the idea of both a sales funnel in marketing and a conversion funnel in digital transformation. Considering the integration of logistics and marketing, authors offer the definition of business echelons as stages of the consumer value creation. The theoretical contribution of this article consists in constructing a mathematical description of business echelons along the entire value chain. The developed analytical description of business echelons is acceptable both for embedding a digital management support system into various software products, and for conducting in-depth analysis and finding optimal solutions.
Circular Economy Business Models for the Tanzanian Coffee Sector: A Teaching Case Study
Authors: Lagrasta Francesco Paolo , Pontrandolfo Pierpaolo , Scozzi Barbara
Published: December 2021

One of the major issues the agri-food supply chains is the considerable production of by-products, which are mostly discarded as wastes and dangerously landfilled. This problem is particularly acute in the coffee supply chain: coffee cultivation generates by-products and in quantities which are potentially dangerous for the environment. A circular economy business model aimed at the recovery of these by-products may represent an interesting solution in terms of environmental, social, and economic sustainability. The goal of this paper was to provide teachers and educators with case material on circular business models that can be used for problem-based learning and case-based learning activities. The proposed case was built to address a real-world problematic situation related to the coffee supply chain. From a theoretical point of view, this study contributes to the literature on circular economy business models by providing a case study developed in the context of a developing country. Furthermore, the research entails practical implications since it shows managers and startuppers how to map a circular business model in all its components under the guidance of a conceptual framework.
AHP, a Reliable Method for Quality Decision Making: A Case Study in Business
Authors: Canco Irina , Kruja Drita , Iancu Tiberiu
Published: December 2021

Decision making is a significant responsibility for business managers, their decisions impacting business performance. Managers are therefore interested in acquiring and implementing reliable methods for making decisions both now and in the future. Currently, in the countries in the Albanian-speaking regions of the Western Balkans, intuitive decision-making methods predominate. In order to find appropriate methods for assessing and prioritizing goals, new approaches to decision making should be adopted. Various methods have been developed for multi-criteria decision making. One of these is the Analytic Hierarchy Process (AHP) method—a method which should receive more attention than it has up to now. We would like to show that the AHP method could be of great use in decision making. Through a case study, this paper explores the AHP, a method with three levels in which the identification of decision-making criteria is based on the perceptions of managers and consumers. The paper’s findings offer an important guide for managers to improve decision making and enhance performance in competitive markets.
Tracing Relationship between Cluster’s Performance and Transition to the Circular Economy
Authors: Razminienė Kristina , Vinogradova-Zinkevič Irina , Tvaronavičienė Manuela
Published: December 2021

Clusters are defined as geographically close groups of organizations that work together to gain a competitive advantage. Clusters’ shared activities involve knowledge sharing, a common pool of resources, innovations, and cooperation. From a more advanced perspective, clusters can work in industrial symbiosis sharing resources, energy, water, and other products. Tendencies of recent research indicate the growing interest in shifting to an efficient use of resources and sustainable development through the circular economy (CE). Clusters can work as enablers of CE to achieve a competitive advantage. The purpose of this study is to trace the relationships between cluster performance and shifting to the CE indicators. Correlation analysis was used as a method to indicate the relationships between pairs of clusters’ performance and shifting to the CE indicators. The limitations of the research refer to the selection of the indicators as both concepts gain insights, although still debatable. The results show that 16 out of 25 cluster performance indicators were identified that have strong or moderate relationships among pairs while shifting to the CE indicators. These indicators are recommended to be included in observation, benchmarking, or evaluation of the clusters’ activities. They can be significant in monitoring the development of shifting to the CE or in combinations with other research areas.
Towards Nearly-Zero Energy in Heritage Residential Buildings Retrofitting in Hot, Dry Climates
Authors: Ibrahim Hanan S. S. , Khan Ahmed Z. , Serag Yehya , Attia Shady
Published: December 2021

Retrofitting “nearly-zero energy” heritage buildings has always been controversial, due to the usual association of the “nearly-zero energy” target with high energy performance and the utilization of renewable energy sources in highly regarded cultural values of heritage buildings. This paper aims to evaluate the potential of turning heritage building stock into a “nearly-zero energy” in hot, dry climates, which has been addressed in only a few studies. Therefore, a four-phase integrated energy retrofitting methodology was proposed and applied to a sample of heritage residential building stock in Egypt along with microscale analysis on buildings. Three reference buildings were selected, representing the most dominant building typologies. The study combines field measurements and observations with energy simulations. In addition, simulation models were created and calibrated based on monitored data in the reference buildings. The results show that the application of hybrid passive and active non-energy generating scenarios significantly impacts energy use in the reference buildings, e.g., where 66.4% of annual electricity use can be saved. Moreover, the application of solar energy sources approximately covers the energy demand in the reference buildings, e.g., where an annual self-consumption of electricity up to 78% and surplus electricity up to 20.4% can be achieved by using photo-voltaic modules. Furthermore, annual natural gas of up to 66.8% can be saved by using two unglazed solar collectors. Lastly, achieving “nearly-zero energy” was possible for the presented case study area. The originality of this work lies in developing and applying an informed retrofitting (nearly-zero energy) guide to be used as a benchmark energy model for buildings that belong to an important historical era. The findings contribute to fill a gap in existing studies of integrating renewable energy sources to achieve “nearly-zero energy” in heritage buildings in hot climates.
Smart Technologies for Sustainable Water Management: An Urban Analysis
Authors: Aivazidou Eirini , Banias Georgios , Lampridi Maria , Vasileiadis Giorgos , Anagnostis Athanasios , Papageorgiou Elpiniki , Bochtis Dionysis
Published: December 2021

As projections highlight that half of the global population will be living in regions facing severe water scarcity by 2050, sustainable water management policies and practices are more imperative than ever. Following the Sustainable Development Goals for equitable water access and prudent use of natural resources, emerging digital technologies may foster efficient monitoring, control, optimization, and forecasting of freshwater consumption and pollution. Indicatively, the use of sensors, Internet of Things, machine learning, and big data analytics has been catalyzing smart water management. With two-thirds of the global population to be living in urban areas by 2050, this research focuses on the impact of digitization on sustainable urban water management. More specifically, existing scientific literature studies were explored for providing meaningful insights on smart water technologies implemented in urban contexts, emphasizing supply and distribution networks. The review analysis outcomes were classified according to three main pillars identified: (i) level of analysis (i.e., municipal or residential/industrial); (ii) technology used (e.g., sensors, algorithms); and (iii) research scope/focus (e.g., monitoring, optimization), with the use of a systematic approach. Overall, this study is expected to act as a methodological tool and guiding map of the most pertinent state-of-the-art research efforts to integrate digitalization in the field of water stewardship and improve urban sustainability.
An Integrated Framework of Green Stormwater Infrastructure Planning—A Review
Authors: Lu Gang , Wang Lin
Published: December 2021

Conventional stormwater management infrastructures show low levels of sustainability owing to the consistent impact of urbanization and climate change, and the green stormwater infrastructure (GSI) has been identified as a more sustainable alternative approach. According to a systematic review, the articles and papers concerning GSI planning are fragmented, especially those discussing the planning steps; thus, an integrated framework of GSI planning is developed here to guide forthcoming planning. In the facility aspect, the research status and prospects of four critical planning steps (i.e., objective formulation, type/scenario evaluation, quantity/scale determination, and site selection) are discussed, and a method of quantifying the relationship between GSI and ecosystem services is given. In the ecosystem aspect, ecosystem resilience promotion is regarded as an approach to guarantee the interaction between hydrological processes and ecological processes, which maintains the sustainable provision of ecosystem services produced by GSI in diverse disturbances. Proposals for future GSI planning research are put forward as comprehensive consideration of the two abovementioned aspects to harvest ecosystem services from GSI directly and to promote the anti-disturbance ability of the ecosystem to guarantee the stable provision of ecosystem services indirectly, which are conducive to the social, economic, and environmental sustainability of GSI.
Applying GMEI-GAN to Generate Meaningful Encrypted Images in Reversible Data Hiding Techniques
Authors: Tsai Chwei-Shyong , Wu Hsien-Chu , Li Yu-Wen , Ying Josh Jia-Ching
Published: December 2021

With the rapid development of information technology, the transmission of information has become convenient. In order to prevent the leakage of information, information security should be valued. Therefore, the data hiding technique has become a popular solution. The reversible data hiding technique (RDH) in particular uses symmetric encoding and decoding algorithms to embed the data into the cover carrier. Not only can the secret data be transmitted without being detected and retrieved completely, but the cover carrier also can be recovered without distortion. Moreover, the encryption technique can protect the carrier and the hidden data. However, the encrypted carrier is a form of ciphertext, which has a strong probability to attract the attention of potential attackers. Thus, this paper uses the generative adversarial networks (GAN) to generate meaningful encrypted images for RDH. A four-stage network architecture is designed for the experiment, including the hiding network, the encryption/decryption network, the extractor, and the recovery network. In the hiding network, the secret data are embedded into the cover image through residual learning. In the encryption/decryption network, the cover image is encrypted into a meaningful image, called the marked image, through GMEI-GAN, and then the marked image is restored to the decrypted image via the same architecture. In the extractor, 100% of the secret data are extracted through the residual learning framework, same as the hiding network. Lastly, in the recovery network, the cover image is reconstructed with the decrypted image and the retrieved secret data through the convolutional neural network. The experimental results show that using the PSNR/SSIM as the criteria, the stego image reaches 45.09 dB/0.9936 and the marked image achieves 38.57 dB/0.9654. The proposed method not only increases the embedding capacity but also maintains high image quality in the stego images and marked images.
Latent Errors and Visible Earned Value: How the Evolutionary Model Integrates Earned Value Metrics with Project System Dynamics
Authors: Nevison John M. , Chichakly Karim J.
Published: December 2021

A project model is presented that weaves together ideas from earned value project management and systems dynamics. It is able to adjust to increasingly unhealthy actual project behaviors in ways that preserve the signature pattern of the staffing histograms observed in the real world and provide a tool for managers to correct projects that are not meeting the plan. Starting from the planned staffing histogram and the project performance baseline, the model captures the delay and cost of experience dilution, includes the unplanned-for effort that is revealed in the typical pattern of the Cost Performance Index, assesses progress using the actual cost to date and the earned value to date, and adjusts staffing, scope, or both, to complete the project on schedule. A new method of approximating work remaining, called project-to-date, is shown to track the planned staffing histogram better than the commonly used fraction-complete method.
Qualitative Heterogeneous Signal Drop on Chemical Shift (CS) MR Imaging: Correlative Quantitative Analysis between CS Signal Intensity Index and Contrast Washout Parameters Using T1-Weighted Sequences
Authors: Stanzione Arnaldo , Verde Francesco , Galatola Roberta , Romeo Valeria , Liuzzi Raffaele , Mainenti Pier Paolo , Aprea Giovanni , Klain Michele , Guadagno Elia , Del Basso De Caro Marialaura , Maurea Simone
Published: December 2021

The aim of this study was to calculate MRI quantitative parameters extracted from chemical-shift (CS) and dynamic contrast-enhanced (DCE) T1-weighted (T1-WS) images of adrenal lesions (AL) with qualitative heterogeneous signal drop on CS T1-WS and compare them to those of AL with homogeneous or no signal drop on CS T1-WS. On 3 T MRI, 65 patients with a total of 72 AL were studied. CS images were qualitatively assessed for grouping AL as showing homogeneous (Group 1, n = 19), heterogeneous (Group 2, n = 23), and no (Group 3, n = 30) signal drop. Histopathology or follow-up data served as reference standard to classify AL. ROIs were drawn both on CS and DCE images to obtain adrenal CS signal intensity index (ASII), absolute (AWO), and relative washout (RWO) values. Quantitative parameters (QP) were compared with ANOVA analysis and post hoc Dunn’s test. The performance of QP to classify AL was assessed with receiver operating characteristic analysis. CS ASII values were significantly different among the three groups (p < 0.001) with median values of 71%, 53%, and 3%, respectively. AWO/RWO values were similar in Groups 1 (adenomas) and 2 (benign AL) but significantly (p < 0.001) lower in Group 3 (20 benign AL and 10 malignant AL). With cut-offs, respectively, of 60% (Group 1 vs. 2), 20% (Group 2 vs. 3), and 37% (Group 1 vs. 3), CS ASII showed areas under the curve of 0.85, 0.96, and 0.93 for the classification of AL, overall higher than AWO/RWO. In conclusion, AL with qualitative heterogeneous signal drop at CS represent benign AL with QP by DCE sequence similar to those of AL with homogeneous signal drop at CS, but different to those of AL with no signal drop at CS; ASII seems to be the only quantitative parameter able to differentiate AL among the three different groups.
Abdominal Imaging Utilization during the First COVID-19 Surge and Utility of Abdominal MRI
Authors: Anderson Mark A. , Goiffon Reece J. , Lennartz Simon , Bhayana Rajesh , Kambadakone Avinash
Published: December 2021

We sought to determine relative utilization of abdominal imaging modalities in coronavirus disease 2019 (COVID-19) patients at a single institution during the first surge and evaluate whether abdominal magnetic resonance imaging (MRI) changed diagnosis and management. 1107 COVID-19 patients who had abdominal imaging were analyzed for modality and imaging setting. Patients who underwent abdominal MRI were reviewed to determine impact on management. Of 2259 examinations, 80% were inpatient, 14% were emergency, and 6% were outpatient consisting of 55% radiograph (XR), 31% computed tomography (CT), 13% ultrasound (US), and 0.6% MRI. Among 1107 patients, abdominal MRI was performed in 12 within 100 days of positive SARS-CoV-2 PCR. Indications were unrelated to COVID-19 in 75% while MRI was performed for workup of acute liver dysfunction in 25%. In 1 of 12 patients, MRI resulted in change to management unrelated to COVID-19 diagnosis. During the first surge of COVID-19 at one institution, the most common abdominal imaging examinations were radiographs and CT followed by ultrasound with the majority being performed as inpatients. Future COVID-19 surges may place disproportionate demands on inpatient abdominal radiography and CT resources. Abdominal MRI was rarely performed and did not lead to change in diagnosis or management related to COVID-19 but needs higher patient numbers for accurate assessment of utility.
Forensic Toxicological and Medico-Legal Evaluation in a Case of Incongruous Drug Administration in Terminal Cancer Patients
Authors: Basilicata Pascale , Giugliano Pasquale , Vacchiano Giuseppe , Simonelli Angela , Guadagni Rossella , Silvestre Angela , Pieri Maria
Published: December 2021

Background: In most cases, palliative care is prescribed to adults diagnosed with cancer. The definition of the most suitable therapy for an effective sedation in terminal cancer patients still represents one of the most challenging goals in medical practice. Due to their poor health, the correct dosing of drugs used for deep palliative sedation in terminal cancer patients, often already on polypharmacological therapy, can be extremely complicated, also considering possible drug-to-drug interactions that could lead to an increased risk of overdose and/or incongruous administration with fatal outcomes. The case of a terminal cancer patient is presented, focusing on the “adequacy” of administered therapy. Materials and Methods: A young male, affected by Ewing sarcoma, attending a palliative care at his own home, died soon after midazolam administration. Toxicological and histological analyses were performed on body fluids and organ fragments. Results and Discussion: Morphological reliefs evidenced a neoplastic mass, composed of lobulated tissue with a lardy, pinkish-gray consistency, extending from the pleural surface to the lung parenchyma, also present at the sacrum region (S1–S5), at the anterior mediastinum level, occupying the entire left pleural cavity, and infiltrating the ipsilateral lung. Metastatic lesions diffused to rachis and lumbar structures. The brain presented edema and congestion. Toxicological analyses evidenced blood midazolam concentrations in the range of 0.931–1.690 µg/mL, while morphine was between 0.266 and 0.909 µg/mL. Death was attributed to cardiorespiratory depression because of a synergic action between morphine and midazolam. The pharmacological interaction between midazolam and morphine is discussed considering the clinical situation of the patient. The opportunity to proceed with midazolam administration is discussed starting from guidelines recommendation. Finally, professional liability outlines are highlighted.
Myocarditis in a Pediatric Patient with Campylobacter Enteritis: A Case Report and Literature Review
Authors: Chantzaras Anastasios-Panagiotis , Karageorgos Spyridon , Panagiotou Panagiota , Georgiadou Elissavet , Chousou Theodora , Spyridopoulou Kalliopi , Paradeisis Georgios , Kanaka-Gantenbein Christina , Botsa Evanthia
Published: December 2021

Myocarditis represents a potential complication of various infectious and noninfectious agents and a common diagnostic challenge for clinicians. Data regarding Campylobacter-associated myocarditis are limited. Here, a case of a 13-year-old female with Campylobacter jejuni gastroenteritis complicated by myocarditis is presented, followed by a literature review in order to retrieve information about Campylobacter-associated carditis in the pediatric population. A search on MEDLINE/PubMed yielded 7relevant cases in the last 20 years. Most of them (six/seven) were males and the mean age was 16.1 years. All patients presented with gastrointestinal symptoms followed in six/seven cases by chest pain within two to seven days. Campylobacter was isolated from stool cultures in six patients; abnormal electrocardiographic findings were detected in six; and abnormal echocardiographic findings in three of the cases. Five patients were treated with antibiotics. Full recovery was the clinical outcome in six patients, whereas one patient died. Concerning the nonspecific symptoms of patients with myocarditis, high clinical suspicion of this complication is necessary in cases where patients with a recent infection present with chest pain and elevated cardiac biomarkers.
Incidence and Outcome of Coinfections with SARS-CoV-2 and Rhinovirus
Authors: Le Glass Elisabeth , Hoang Van Thuan , Boschi Céline , Ninove Laetitia , Zandotti Christine , Boutin Aurélie , Bremond Valérie , Dubourg Grégory , Ranque Stéphane , Lagier Jean-Christophe , Million Matthieu , Fournier Pierre-Edouard , Drancourt Michel , Gautret Philippe , Colson Philippe
Published: December 2021

Background: We aimed to compare the clinical severity in patients who were coinfected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and rhinovirus or monoinfected with a single one of these viruses. Methods: The study period ranged from 1 March 2020 to 28 February 2021 (one year). SARS-CoV-2 and other respiratory viruses were identified by real-time reverse-transcription-PCR as part of the routine work at Marseille University hospitals. Bacterial and fungal infections were detected by standard methods. Clinical data were retrospectively collected from medical files. This study was approved by the ethical committee of our institute. Results: A total of 6034/15,157 (40%) tested patients were positive for at least one respiratory virus. Ninety-three (4.3%) SARS-CoV-2-infected patients were coinfected with another respiratory virus, with rhinovirus being the most frequent (62/93, 67%). Patients coinfected with SARS-CoV-2 and rhinovirus were significantly more likely to report a cough than those with SARS-CoV-2 monoinfection (62% vs. 31%; p = 0.0008). In addition, they were also significantly more likely to report dyspnea than patients with rhinovirus monoinfection (45% vs. 36%; p = 0.02). They were also more likely to be transferred to an intensive care unit and to die than patients with rhinovirus monoinfection (16% vs. 5% and 7% vs. 2%, respectively) but these differences were not statistically significant. Conclusions: A close surveillance and investigation of the co-incidence and interactions of SARS-CoV-2 and other respiratory viruses is needed. The possible higher risk of increased clinical severity in SARS-CoV-2-positive patients coinfected with rhinovirus warrants further large scale studies.
Molecular Characterization of Small Ruminant Lentiviruses in Polish Mixed Flocks Supports Evidence of Cross Species Transmission, Dual Infection, a Recombination Event, and Reveals the Existence of New Subtypes within Group A
Authors: Olech Monika , Kuźmak Jacek
Published: December 2021

Small ruminant lentiviruses (SRLVs) are a group of highly divergent viruses responsible for global infection in sheep and goats. In a previous study we showed that SRLV strains found in mixed flocks in Poland belonged to subtype A13 and A18, but this study was restricted only to the few flocks from Małopolska region. The present work aimed at extending earlier findings with the analysis of SRLVs in mixed flocks including larger numbers of animals and flocks from different part of Poland. On the basis of gag and env sequences, Polish SRLVs were assigned to the subtypes B2, A5, A12, and A17. Furthermore, the existence of a new subtypes, tentatively designed as A23 and A24, were described for the first time. Subtypes A5 and A17 were only found in goats, subtype A24 has been detected only in sheep while subtypes A12, A23, and B2 have been found in both sheep and goats. Co-infection with strains belonging to different subtypes was evidenced in three sheep and two goats originating from two flocks. Furthermore, three putative recombination events were identified within gag and env SRLVs sequences derived from three sheep. Amino acid (aa) sequences of immunodominant epitopes in CA protein were well conserved while Major Homology Region (MHR) had more alteration showing unique mutations in sequences of subtypes A5 and A17. In contrast, aa sequences of surface glycoprotein exhibited higher variability confirming type-specific variation in the SU5 epitope. The number of potential N-linked glycosylation sites (PNGS) ranged from 3 to 6 in respective sequences and were located in different positions. The analysis of LTR sequences revealed that sequences corresponding to the TATA box, AP-4, AML-vis, and polyadenylation signal (poly A) were quite conserved, while considerable alteration was observed in AP-1 sites. Interestingly, our results revealed that all sequences belonging to subtype A17 had unique substitution T to A in the fifth position of TATA box and did not have a 11 nt deletion in the R region which was noted in other sequences from Poland. These data revealed a complex picture of SRLVs population with ovine and caprine strains belonging to group A and B. We present strong and multiple evidence of dually infected sheep and goats in mixed flocks and present evidence that these viruses can recombine in vivo.
Integrating High throughput Sequencing into Survey Design Reveals Turnip Yellows Virus and Soybean Dwarf Virus in Pea (Pisum Sativum) in the United Kingdom
Authors: Fowkes Aimee R. , McGreig Sam , Pufal Hollie , Duffy Shona , Howard Becky , Adams Ian P. , Macarthur Roy , Weekes Rebecca , Fox Adrian
Published: December 2021

There is only limited knowledge of the presence and incidence of viruses in peas within the United Kingdom, therefore high-throughput sequencing (HTS) in combination with a bulk sampling strategy and targeted testing was used to determine the virome in cultivated pea crops. Bulks of 120 leaves collected from twenty fields from around the UK were initially tested by HTS, and presence and incidence of virus was then determined using specific real-time reverse-transcription PCR assays by testing smaller mixed-bulk size samples. This study presents the first finding of turnip yellows virus (TuYV) in peas in the UK and the first finding of soybean dwarf virus (SbDV) in the UK. While TuYV was not previously known to be present in UK peas, it was found in 13 of the 20 sites tested and was present at incidences up to 100%. Pea enation mosaic virus-1, pea enation mosaic virus-2, pea seed-borne mosaic virus, bean yellow mosaic virus, pea enation mosaic virus satellite RNA and turnip yellows virus associated RNA were also identified by HTS. Additionally, a subset of bulked samples were re-sequenced at greater depth to ascertain whether the relatively low depth of sequencing had missed any infections. In each case the same viruses were identified as had been identified using the lower sequencing depth. Sequencing of an isolate of pea seed-borne mosaic virus from 2007 also revealed the presence of TuYV and SbDV, showing that both viruses have been present in the UK for at least a decade, and represents the earliest whole genome of SbDV from Europe. This study demonstrates the potential of HTS to be used as a surveillance tool, or for crop-specific field survey, using a bulk sampling strategy combined with HTS and targeted diagnostics to indicate both presence and incidence of viruses in a crop.
Nanopore-Based Direct RNA-Sequencing Reveals a High-Resolution Transcriptional Landscape of Porcine Reproductive and Respiratory Syndrome Virus
Authors: Zhang Riteng , Wang Peixin , Ma Xin , Wu Yifan , Luo Chen , Qiu Li , Zeshan Basit , Yang Zengqi , Zhou Yefei , Wang Xinglong
Published: December 2021

The TRS-mediated discontinuous transcription process is a hallmark of Arteriviruses. Precise assessment of the intricate subgenomic RNA (sg mRNA) populations is required to understand the kinetics of viral transcription. It is difficult to reconstruct and comprehensively quantify splicing events using short-read sequencing, making the identification of transcription-regulatory sequences (TRS) particularly problematic. Here, we applied long-read direct RNA sequencing to characterize the recombined RNA molecules produced in porcine alveolar macrophages during early passage infection of porcine reproductive and respiratory syndrome virus (PRRSV). Based on sequencing two PRRSV isolates, namely XM-2020 and GD, we revealed a high-resolution and diverse transcriptional landscape in PRRSV. The data revealed intriguing differences in subgenomic recombination types between the two PRRSVs while also demonstrating TRS-independent heterogeneous subpopulation not previously observed in Arteriviruses. We find that TRS usage is a regulated process and share the common preferred TRS in both strains. This study also identified a substantial number of TRS-mediated transcript variants, including alternative-sg mRNAs encoding the same annotated ORF, as well as putative sg mRNAs encoded nested internal ORFs, implying that the genetic information encoded in PRRSV may be more intensively expressed. Epigenetic modifications have emerged as an essential regulatory layer in gene expression. Here, we gained a deeper understanding of m5C modification in poly(A) RNA, elucidating a potential link between methylation and transcriptional regulation. Collectively, our findings provided meaningful insights for redefining the transcriptome complexity of PRRSV. This will assist in filling the research gaps and developing strategies for better control of the PRRS.
Cryo-EM Structures of Two Bacteriophage Portal Proteins Provide Insights for Antimicrobial Phage Engineering
Authors: Javed Abid , Villanueva Hugo , Shataer Shadikejiang , Vasciaveo Sara , Savva Renos , Orlova Elena V.
Published: December 2021

Widespread antibiotic resistance has returned attention to bacteriophages as a means of managing bacterial pathogenesis. Synthetic biology approaches to engineer phages have demonstrated genomic editing to broaden natural host ranges, or to optimise microbicidal action. Gram positive pathogens cause serious pastoral animal and human infections that are especially lethal in newborns. Such pathogens are targeted by the obligate lytic phages of the Salasmaviridae and Guelinviridae families. These phages have relatively small ~20 kb linear protein-capped genomes and their compact organisation, relatively few structural elements, and broad host range, are appealing from a phage-engineering standpoint. In this study, we focus on portal proteins, which are core elements for the assembly of such tailed phages. The structures of dodecameric portal complexes from Salasmaviridae phage GA1, which targets Bacillus pumilus, and Guelinviridae phage phiCPV4 that infects Clostridium perfringens, were determined at resolutions of 3.3 Å and 2.9 Å, respectively. Both are found to closely resemble the related phi29 portal protein fold. However, the portal protein of phiCPV4 exhibits interesting differences in the clip domain. These structures provide new insights on structural diversity in Caudovirales portal proteins and will be essential for considerations in phage structural engineering.
Effectiveness of the WHO-Authorized COVID-19 Vaccines: A Rapid Review of Global Reports till 30 June 2021
Authors: Cheng Chang-Jie , Lu Chun-Yi , Chang Ya-Hui , Sun Yu , Chu Hai-Jui , Lee Chun-Yu , Liu Chang-Hsiu , Lin Cheng-Huai , Lu Chien-Jung , Li Chung-Yi
Published: December 2021

Large clinical trials have proven the efficacy of the COVID-19 vaccine, and the number of studies about the effectiveness rapidly grew in the first half of the year after mass vaccination was administrated globally. This rapid review aims to provide evidence syntheses as a means to complement the current evidence on the vaccine effectiveness (VE) against various outcomes in real-world settings. Databases (PubMed, EMBASE, and MedRxiv) were searched up to 30 June 2021, (PROSPERO ID: 266866). A total of 39 studies were included, covering over 15 million participants from 11 nations. Among the general population being fully vaccinated, the VE against symptomatic SARS-CoV-2 infection was estimated at 89–97%, 92% (95% CI, 78–97%), and 94% (95% CI, 86–97%) for BNT162b2, ChAdOx1, and mRNA-1273, respectively. As for the protective effects against B.1.617.2-related symptomatic infection, the VE was 88% (95% CI, 85.3–90.1%) by BNT162b2 and 67.0% (95% CI, 61.3–71.8%) by ChAdOx1 after full vaccination. This review revealed a consistently high effectiveness of certain vaccines among the general population in real-world settings. However, scarce data on the major variants of SARS-CoV-2 and the shortness of the study time may limit the conclusions to the mRNA vaccines and ChAdOx1.
Immunogenicity of a DNA-Based Sindbis Replicon Expressing Crimean–Congo Hemorrhagic Fever Virus Nucleoprotein
Authors: Tipih Thomas , Heise Mark , Burt Felicity Jane
Published: December 2021

Crimean–Congo hemorrhagic fever virus (CCHFV) infrequently causes hemorrhagic fever in humans with a case fatality rate of 30%. Currently, there is neither an internationally approved antiviral drug nor a vaccine against the virus. A replicon based on the Sindbis virus vector encoding the complete open reading frame of a CCHFV nucleoprotein from a South African isolate was prepared and investigated as a possible candidate vaccine. The transcription of CCHFV RNA and recombinant protein production by the replicon were characterized in transfected baby hamster kidney cells. A replicon encoding CCHFV nucleoprotein inserted in plasmid DNA, pSinCCHF-52S, directed transcription of CCHFV RNA in the transfected cells. NIH-III heterozygous mice immunized with pSinCCHF-52S generated CCHFV IgG specific antibodies with notably higher levels of IgG2a compared to IgG1. Splenocytes from mice immunized with pSinCCHF-52S secreted IFN-γ and IL-2, low levels of IL-6 or IL-10, and no IL-4. No specific cytokine production was registered in splenocytes of mock-immunized mice (p < 0.05). Thus, our study demonstrated the expression of CCHFV nucleoprotein by a Sindbis virus vector and its immunogenicity in mice. The spectrum of cytokine production and antibody profile indicated predominantly Th1-type of an anti-CCHFV immune response. Further studies in CCHFV-susceptible animals are necessary to determine whether the induced immune response is protective.