bluuebunny/crossref_metadata_2025 huggingface dataset:
The application value of metagenomic next-generation sequencing in community-acquired purulent meningitis after antibiotic intervention
Authors: Shangguan Lijuan , Xue Lanping , Shang Jing , Wang Hailong
Published: October 2023

Background

Bacteria account for nearly one third of the causes of community-acquired central nervous system infections, and traditional diagnostic methods are based on culture results, which are time-consuming and have a low detection rate leading to delayed diagnosis and treatment. Since metagenomic next-generation sequencing (mNGS) has the advantages of high timeliness and only detecting microbial trace gene fragments, it has been used more widely in recent years. Based on this, we explored whether the application of cerebrospinal fluid (CSF) mNGS is advantageous in patients with community-acquired purulent meningitis, especially in people who have already used antibiotics.

Methods

This was a retrospective study of 63 patients with community-acquired purulent meningitis admitted to the Department of Neurology of Shanxi Bethune Hospital from March 2018 to November 2022. Data were systematically collected and classified into CSF culture group, blood culture group and CSF mNGS group according to different detection methods, and the total detection rate of each method was calculated. Each group of patients was divided into two subgroups according to whether antibiotics were used before sampling. The detection rates of the three groups were compared within and between groups to explore whether mNGS has advantages over traditional methods and the influence of antibiotic use on detection rates of the three methods.

Results

Among the 63 patients, the cases of CSF culture, blood culture and CSF mNGS were 56, 46, 44, respectively. The total detection rates of the three methods were 17.86%, 36.96%, 81.82%, with statistical differences (p < 0.05),suggesting that the detection rate of mNGS was higher than CSF culture (p < 0.05) and blood culture (p < 0.05),and the detection rate of blood culture higher than CSF culture (p < 0.05). Further grouping found that without antibiotics, the detection rates of CSF culture, blood culture and CSF mNGS were 28.57%, 56.25% and 88.89%, with statistical differences (p < 0.05), and the detection rate of CSF mNGS was higher than that of CSF culture (p < 0.05), but there was no statistical difference between CSF and blood culture (p > 0.05), nor between blood culture and CSF mNGS (p > 0.05). The detection rates of the three groups with antibiotics were 14.29%, 26.67% and 80.00%, with statistical differences (p < 0.05), and the detection rate of CSF mNGS was still higher than CSF culture (p < 0.05) and blood culture (p < 0.05). However, the detection rate of CSF mNGS also decreased after antibiotics were used for more than 3 days.

Conclusions

The detection rate of CSF mNGS in patients with purulent meningitis is higher than traditional methods, especially in patients who have been given antibiotics, but the detection rate will decrease with the extension of antibiotic use.
Cholinergic signaling influences the expression of immune checkpoint inhibitors, PD-L1 and PD-L2, and tumor hallmarks in human colorectal cancer tissues and cell lines
Authors: Kuol Nyanbol , Godlewski Janusz , Kmiec Zbigniew , Vogrin Sara , Fraser Sarah , Apostolopoulos Vasso , Nurgali Kulmira
Published: October 2023

Background

Cancer cells express immunosuppressive molecules, such as programmed death ligands (PD-L)1 and PD-L2, enabling evasion from the host’s immune system. Cancer cells synthesize and secrete acetylcholine (ACh), acting as an autocrine or paracrine hormone to promote their proliferation, differentiation, and migration.

Methods

We correlated the expression of PD-L1, PD-L2, cholinergic muscarinic receptor 3 (M3R), alpha 7 nicotinic receptor (α7nAChR), and choline acetyltransferase (ChAT) in colorectal cancer (CRC) tissues with the stage of disease, gender, age, risk, and patient survival. The effects of a muscarinic receptor blocker, atropine, and a selective M3R blocker, 4-DAMP, on the expression of immunosuppressive and cholinergic markers were evaluated in human CRC (LIM-2405, HT-29) cells.

Results

Increased expression of PD-L1, M3R, and ChAT at stages III-IV was associated with a high risk of CRC and poor survival outcomes independent of patients’ gender and age. α7nAChR and PD-L2 were not changed at any CRC stages. Atropine and 4-DAMP suppressed the proliferation and migration of human CRC cells, induced apoptosis, and decreased PD-L1, PD-L2, and M3R expression in CRC cells via inhibition of EGFR and phosphorylation of ERK.

Conclusions

The expression of immunosuppressive and cholinergic markers may increase the risk of recurrence of CRC. These markers might be used in determining prognosis and treatment regimens for CRC patients. Blocking cholinergic signaling may be a potential therapeutic for CRC through anti-proliferation and anti-migration via inhibition of EGFR and phosphorylation of ERK. These effects allow the immune system to recognize and eliminate cancer cells.
Associations between serum estradiol and IL-6/sIL-6R/sgp130 complex in female patients with major depressive disorder
Authors: Sun Ting , Chen Qian , Mei Junchi , Li Yan
Published: October 2023

Background

It has been hypothesized that the IL-6/sIL-6R/sgp130 complex, an inflammatory complex, plays a critical role in the pathogenesis of major depressive disorder (MDD). Estradiol (E2) is a sex steroid hormone involved in emotional regulation and MDD. This study aimed to investigate the relationship between E2 and IL-6/sIL-6R/sgp130 complex in patients with MDD.

Methods

Using enzyme-linked immunosorbent assay, the levels of IL-6, sIL-6Rα, and sgp130 were compared between 117 female patients with MDD and 122 healthy controls.The serum concentrations of E2 and other biomarkers were also measured.

Results

(1) The serum levels of IL-6 and sIL-6Rα in patients with MDD were significantly higher than those in the control group, while the serum levels of sgp130 and E2 were significantly lower (all P < 0.05). (2) Low levels of E2 were associated with high levels of IL-6 and low levels of sgp130 (all P < 0.01). (3) HAMD-24 score was positively correlated with the serum level of IL-6, but negatively correlated with the serum levels of sgp130 and E2(all P < 0.05). (4) IL-6 and sgp130 had certain prognostic values in MDD, and the combination of various indicators showed a significantly superior prognostic value.

Conclusions

The IL6/sIL-6R/sgp130 complex in female patients with MDD was closely related to E2 level. In addition, IL-6 and sgp130 may be valuable serum biomarkers for the diagnosis and prognosis of MDD in women.
Association between different insulin resistance surrogates and infertility in reproductive-aged females
Authors: Xia Weiting , Cai Yaoyao , Zhang Sisi , Wu Shenghao
Published: October 2023

Background

Obesity and metabolic syndrome are observed more frequently in infertile women, and insulin resistance (IR) is closely related to them. However, there are no studies that have examined the association between different IR surrogates and female infertility, hence we investigated the potential association between them in the general population.

Methods

This was a cross-sectional study using data from the National Health and Nutrition Examination Survey (NHANES, 2013–2018). The association of different IR surrogates (HOMA-IR index, TyG index and TyG-BMI index) with female infertility was estimated by multivariable regression analysis.

Results

After adjusting for confounders, the HOMA-IR index and TyG index did not show an association with female infertility, while the TyG-BMI index was found to have a positive association with female infertility (OR = 1.01, 95% CI: 1.00, 1.01; P < 0.0001), and the OR of the TyG-BMI group T3 (≥ 255.55) was significantly different compared to the group T1 (< 185.31) (OR = 3.02, 95% CI: 1.62, 5.60). Similar results were seen in most of the subgroup participants by stratified analysis (P-interaction > 0.05). However, different IR surrogates did not show variability in their ability to predict infertility [TyG-BMI: 0.68 (95% CI: 0.62, 0.74) vs. TyG: 0.62 (95% CI: 0.57, 0.68) vs. HOMA-IR: 0.65 (95% CI: 0.60, 0.71)].

Conclusions

Our result suggests that high levels of TyG-BMI index were positively associated with female infertility in US reproductive-aged females.
A novel concentrated growth factor (CGF) and bio-oss based strategy for second molar protection after impacted mandibular third molar extraction: a randomized controlled clinical study
Authors: Sun Shoufu , Xu Xiaodong , Zhang Zhongxiao , Zhang Ying , Wei Wenjia , Guo Ke , Jiang Yunan N.
Published: October 2023

Background

The extraction of impacted mandibular third molars might cause large bone defects in the distal area of second molars. A new strategy was innovatively employed here combining autologous bone, Bio-Oss, concentrated growth factors (CGF) gel and CGF membrane for bone repair, and the present study aimed at exploring safety as well as short- and long-term efficacy of this new protocol clinically.

Materials and methods

A total of 66 participants were enrolled in this randomized single-blind clinical trial, and randomly allocated to control group (only blood clots), test A group (autogenous bone, Bio-Oss with barrier membrane) and test B group (autogenous bone, Bio-Oss, CGF gel with CGF membrane). The postoperative outcomes including PoSSe scale, periodontal probing depth (PD), degree of gingival recession and computed tomography measurements were assessed at 3rd, 6th, 12th month. A p-value < 0.05 was considered statistically significant.

Results

In PoSSe scale, no significant difference was observed except a significant alleviation of early-stage pain perception in test B group (p < 0.05). Also, test B group exhibited better effect on periodontal healing and gingival recession reduction after 6 months (p < 0.05). Both two test groups showed more new bone formation than the control group (p < 0.05). It is noteworthy that the bone repair of test B group was significantly better than that of test A at 3rd and 6th month (p < 0.05), yet no difference was observed at 12th month (p > 0.05).

Conclusion

Both two test groups could achieve stable long-term efficacy on bone defect repair. The use of CGF gel and CGF membrane could accelerate early-stage bone repair, alleviate short-term pain after surgery, reduce long-term probing depth and relieve economic cost for patients. This new bone repair protocol is worthy of promoting by clinicians.

Trial registration

This study was registered with the identification number ChiCTR2300068466 on 20/02/2023 at Chinese Clinical Trial Registry. Also, it was ethically approved from the institutional ethics committee at the Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (No:2023-010-01), and has been conducted in accordance to the guidelines of the declaration of Helsinki. Written informed consent was obtained from all participants in the study.
The applicability of the “surprise question” as a prognostic tool in patients with severe chronic comorbidities in a university teaching outpatient setting
Authors: Lin C. A. , Pires P. P. , Freitas L. V. , Reis P. V. S. , Silva F. D. , Herbst L. G. , Nunes R. , Lin C. J. , Nunes M. P. T.
Published: October 2023

Background

Life expectancy in recent decades has increased the prevalence of chronic diseases in the population, requiring an approach to new health topics, such as discussions on quality of life and expectations about death and dying. The concept of advance directives (ADs) gives individuals the opportunity to make known their decisions about the treatments they would like to receive at the end of life. Despite the recognition of relevance in clinical practice, the applicability of the concept presents challenges, including establishing the appropriate prognosis for each patient and the ideal time to approach the patient. Some prognostic tools were developed, such as the surprise question (SQ): “Would you be surprised if your patient died in 12 months?”, which is used in some clinical settings to predict patient deaths and to make decisions regarding ADs. The main objective of the present study was to evaluate the behavior of second-year resident physicians (PGY-2) when the SQ was applied.

Method

In our observational study, from July 1, 2016, to February 28, 2017, (PGY-2) in the Internal Medicine Residency Program (IMRP) applied SQ to all patients with multiple and varied chronic no communicable comorbidities, who were followed up at the general medicine outpatient clinic (GMOC) of a tertiary university hospital in São Paulo- Brazil. The frequency of the outcome (death or non-death within 12 months) was analyzed by correlating it with the clinical data (impact of the studied variables).

Results

Eight hundred forty patients entered the study. Fitfty-two of them (6.2%) died within one year. PGY-2 predicted that two hundred and fourteen patients (25.5% of total) would die within a year (answer No to SQ), of which, 32 (14.9%) did so. The correct residents’ prognosis for the subgroup of 626 patients (answer “Yes” to SQ) was NPV = 96.8% (CI = 95.4%-98.2%) and PPV = 14.9% (CI 10.1%-19, 6%). Answering “Yes” to SQ correlated negatively to addressing AD while the outcomes death and the answer No to SQ were positively correlated, according to the number of comorbidities.

Conclusion

The SQ, in addition to care, contributed to health education, communication and care planning shared by the doctor and patient.
Analysing the progress in service delivery towards achieving universal health coverage in Nigeria: a scoping review
Authors: Okeke Chinyere , Ezenwaka Uchenna , Ekenna Adanma , Onyedinma Chioma , Onwujekwe Obinna
Published: October 2023

Background

Attainment of universal health coverage (UHC) requires optimal utilization of health services. Poor coverage and inequitable access to healthcare could hinder improvement in service delivery towards UHC. The study analyzed the progress in service delivery coverage and equity in access to care within the Nigerian health systems based on the tracer indicators of the WHO framework for monitoring UHC.

Methods

We searched the literature in databases: PubMed, Scopus, Directory of Open Access Journals, Google Scholar, Science Direct and websites of relevant health Ministries, Agencies, and Organizations between March to December 2022. Search terms were identified in four broader themes: Service delivery coverage, equity, UHC and Nigeria. Data were collected through a review of 37 published articles (19 peer-reviewed articles and 8 grey documents). We synthesized the findings in thematic areas using the WHO framework for monitoring UHC.

Results

The findings show a slow improvement in service delivery coverage across the UHC tracer indicators; reproductive, maternal, newborn and child health, infectious diseases, non-communicable diseases and service capacity and access. With regards to equity in access to care across the tracer indicators, there has been a great disparity in the utilization of healthcare services among rural dwellers, lower educational level individuals and those with poor socio-economic status over 20 years. However, there was remarkable progress in the ownership and use of long-lasting insecticide-treated nets among rural and lowest-wealth quantile households than their urban counterpart.

Conclusion

There is poor coverage and persistent inequitable access to care among the tracer indicators for monitoring progress in service delivery. Attaining UHC requires concerted efforts and investment of more resources in service delivery to address inequitable access to care and sustainable service coverage for improved health outcomes.
Impact of a combination of pimobendan, furosemide, and enalapril on heart rate variability in naturally occurring, symptomatic, myxomatous mitral valve degeneration dogs
Authors: Pirintr Prapawadee , Saengklub Nakkawee , Boonpala Pakit , Hamlin Robert L. , Kijtawornrat Anusak
Published: October 2023

Background

Pimobendan, diuretics, and an angiotensin-converting enzyme inhibitor (ACEi) are widely used for the management of chronic valvular heart disease in dogs; however, the effects of that combination on heart rate variability (HRV) are unknown. The purpose of this study was to assess the HRV of symptomatic myxomatous mitral valve degeneration (MMVD) dogs in response to therapy with a combination of pimobendan, diuretics, and ACEi.

Results

MMVD stage C (n = 17) dogs were enrolled and a 1-hour Holter recording together with echocardiography, blood pressure measurement, and blood chemistry profiles were obtained before and 1, 3, and 6 months after oral treatment with pimobendan (0.25 mg/kg), enalapril (0.5 mg/kg), and furosemide (2 mg/kg) twice daily. The results revealed that MMVD stage C dogs at the baseline had lower values of time-domain indices, low frequency (LF), high frequency (HF), and total power, as well as higher value of LF/HF. Triple therapy significantly increases these parameters in MMVD stage C dogs (P < 0.05). A positive moderate correlation was observed between time domain parameters and a left ventricular internal diastole diameter normalized to body weight (P < 0.05).

Conclusions

It can be concluded that MMVD stage C dogs possess low HRV due to either the withdrawal of parasympathetic tone or enhanced sympathetic activation, and a combination therapy was shown to enhance cardiac autonomic modulation inferred from the increased heart rate variability. Therefore, a combination therapy may be useful for restoring normal autonomic nervous system activity in dogs with MMVD stage C.
Localization of coronary bypass targets in hard-to-see coronary arteries
Authors: Gerrah Rabin , Lipe Kristin , Vlahakes Gus J.
Published: October 2023

Background

Precise identification of coronary arteries and selection of anastomotic sites are critical stages of coronary bypass surgery. Visualization of coronary arteries is occasionally challenging when the heart is covered with a thick layer of fat or scar tissue. In this paper, we review the methods to localize the coronary arteries during coronary surgery.

Methods

Prior publications were searched to summarize all available methods for localization of coronary arteries during coronary surgery.

Results

Five clinically recognized and three experimental techniques from the literature review are reviewed and summarized.

Conclusions

Knowledge of various techniques of coronary artery identification in hard-to-see coronary arteries is an important asset in coronary surgery and especially useful during the most critical option of the most common heart surgery.
Monitoring bovine dairy calf health and related risk factors in the first three months of rearing
Authors: Otten Nina Dam , Skarbye Alice Puk , Krogh Mogens Agerbo , Michelsen Anne Marie , Nielsen Liza Rosenbaum
Published: October 2023

Background

Rearing replacement heifers is pivotal for the dairy industry and is associated with high input costs for the preweaned calves, due to their higher susceptibility to diseases. Ensuring calf health and viability calls for systematic approaches in order to mitigate the costs induced by managing sick calves and to ensure animal welfare. The objective of this study was to develop a systematic and feasible health-monitoring tool for bovine dairy calves based on repeated clinical observations and diagnostic results of calves at three time points; the 1st (T0), the 3rd (T1) and the 12th (T3) week of age. The study included observations from 77 dairy heifer calves in nine Danish commercial dairy herds. Immunisation status was assessed by serum Brix% at T0. Clinical scoring included gastrointestinal disease (GD) and respiratory disease (RD). The average daily weight gain (ADWG) was estimated from heart-girth measurements. Pathogen detection from nasal swabs and faecal samples were analysed for 16 respiratory and enteric pathogens by means of high-throughput real time-PCR. All measures obtained in each herd were visualised in a panel to follow the health status of each calf over time.

Results

The individual clinical observations combined with diagnostic information from immunisation and pathogen detection form each enrolled calf are presented in a herd dashboard illustrating the health status over the study period. This monitoring revealed failure of passive transfer (Brix% < 8.1) in 31% of the 77 enrolled calves, signs of severe GD peaked at T0 with 20% affected calves, while signs of severe RD peaked at T2 with 42% affected calves. ADWG over the first eight weeks was estimated to be 760 g (± 190 g). Pathogen profiles varied between herds.

Conclusions

The large variation in both clinical disease and pathogen occurrence across herds emphasizes the need for herd specific monitoring. Combining the results of the present study from measures of immunisation, health and growth from individual calves in one visualisation panel allowed for the detection of patterns across age groups in the specific herds, showing promising potential for early detection and interventions that can lead to enhanced calf health and welfare.
Randomized phase III trial evaluating motivational interviewing and text interventions to optimize adherence to breast cancer endocrine therapy (Alliance A191901): the GETSET protocol
Authors: Ivory Joannie , Wheeler Stephanie B. , Drier Sarah , Gunn Heather , Zahrieh David , Paskett Electra , Naughton Michelle , Wills Rachel , Swetel Kayla , Chow Selina , Reeder-Hayes Katherine
Published: October 2023

Background

Hormone receptor-positive (HR +) breast cancer is the most common type of breast cancer in the USA but has excellent long-term outcomes in recent decades, in part due to effective oral endocrine therapy (ET). ET medications are typically prescribed for 5 to 10 years, depending on the risk of recurrence, and must be taken daily. One limiting factor to ET efficacy is nonadherence, with high-risk groups for nonadherence including younger women and Black women.

Methods

The Alliance for Clinical Trials in Oncology (Alliance) trial A191901 is an ongoing, four-arm (text message reminder (TMR), motivational interviewing (MI), TMR plus MI, or enhanced usual care) randomized clinical trial that tests the efficacy and effect of two interventions (TMR and/or MI) on improved ET adherence, patient-reported outcomes (PROs), and resource use requirements among HR + breast cancer survivors. Participants are randomized in a 1:1:1:1 ratio to the four arms. With an assumed loss to follow-up of approximately 11%, we plan to recruit 1180 participants. Randomization is stratified based on age and race to ensure balance between the arms, and we oversample younger and Black women, with each group representing 30% of the study population. Participants randomized to an intervention will actively participate in the intervention for 9 months, and all participants will be followed for adherence data and PRO endpoints, through the use of the Pillsy cap medication event monitoring system and Alliance ePRO survey app (i.e., Patient Cloud). The primary analysis will compare Pillsy-measured ET adherence among study arms at 12 months.

Discussion

This multisite study will not only define strategies to improve adherence to breast cancer oral therapies, but it will also potentially support strategies in large cooperative research groups that can increase delivery and tolerability of ET, involve diverse patient populations in clinical research, and engage patients effectively in interventional studies, using remote and cost-effective delivery methods.

Trial registration

Clinicaltrials.gov NCT04379570. Registered on 7 May 2020.
Injuries in professional women’s elite soccer players in Kosovo: epidemiological injury study
Authors: Gashi Feim , Kovačič Tine , Shalaj Ismet , Haxhiu Bekim , Boshnjaku Arben
Published: October 2023

Background

An emphasis has been given lately towards women’s engagement together with their potential in soccer. As this sport develops with athletes becoming more physically fit and skilled, it is unclear what the consequences in terms of injuries are. Having this in mind, this study aimed to investigate the major injuries that occur in women’s soccer players.

Methods

This descriptive epidemiological study invited all 286 women’s soccer players from the 12 participating women clubs in the Kosovo 1st Soccer League (elite football level) during the 2021/2022 season, out of which 142 from 12 clubs participated. Exposure time for 1000 h of playing and training were recorded in addition to the anthropometric data, playing position, and prior injury history during the end of the season, practice, and match. The exact type of injury, severity, and post-injury recovery time, as well as the circumstances surrounding the injuries, were recorded.

Results

In total 84 injuries were registered with an overall injury ratio (IR) being 3.21 (CI: 2.56, 3.98) injuries/1000 exposure hours. During the competitive season, each player sustained 1.4 injuries on average. IRs were significantly higher during competition (n = 50; IR = 1.57; CI: 1.52, 1.62) compared to training (n = 34; IR = 0.26, CI: 0.25, 0.27). Out of a total of 142 women players, 84 (59.2%) injuries occurred, and no record of injuries was made in 58 (40.8%) players. The overall IR was observed to be 3.21 (CI: 1.24, 3.27), with moderate and severe injuries accounting for 38.1% of total injuries (each), followed by mild (16.7%) and minimal (7.1%) injuries.

Conclusion

The women IR in Kosovo women’s soccer players is low while being circa 11% below the international average. Almost 2 out of every 4 injuries were categorized as traumatic, with the IRs being more than 5-fold larger during games than during training. Additionally, these findings emphasize the higher rate of injuries amongst younger athletes, suggesting caution to be taken by the coaches when planning for the match. The collected data may help coaches and trainers create more targeted women’s soccer injury prevention programs.
Bloom filters for molecules
Authors: Medina Jorge , White Andrew D.
Published: October 2023

Ultra-large chemical libraries are reaching 10s to 100s of billions of molecules. A challenge for these libraries is to efficiently check if a proposed molecule is present. Here we propose and study Bloom filters for testing if a molecule is present in a set using either string or fingerprint representations. Bloom filters are small enough to hold billions of molecules in just a few GB of memory and check membership in sub milliseconds. We found string representations can have a false positive rate below 1% and require significantly less storage than using fingerprints. Canonical SMILES with Bloom filters with the simple FNV (Fowler-Noll-Voll) hashing function provide fast and accurate membership tests with small memory requirements. We provide a general implementation and specific filters for detecting if a molecule is purchasable, patented, or a natural product according to existing databases at https://github.com/whitead/molbloom.
Deep encoder/decoder dual-path neural network for speech separation in noisy reverberation environments
Authors: Wang Chunxi , Jia Maoshen , Zhang Xinfeng
Published: October 2023

In recent years, the speaker-independent, single-channel speech separation problem has made significant progress with the development of deep neural networks (DNNs). However, separating the speech of each interested speaker from an environment that includes the speech of other speakers, background noise, and room reverberation remains challenging. In order to solve this problem, a speech separation method for a noisy reverberation environment is proposed. Firstly, the time-domain end-to-end network structure of a deep encoder/decoder dual-path neural network is introduced in this paper for speech separation. Secondly, to make the model not fall into local optimum during training, a loss function stretched optimal scale-invariant signal-to-noise ratio (SOSISNR) was proposed, inspired by the scale-invariant signal-to-noise ratio (SISNR). At the same time, in order to make the training more appropriate to the human auditory system, the joint loss function is extended based on short-time objective intelligibility (STOI). Thirdly, an alignment operation is proposed to reduce the influence of time delay caused by reverberation on separation performance. Combining the above methods, the subjective and objective evaluation metrics show that this study has better separation performance in complex sound field environments compared to the baseline methods.
Participant attention on the intervention target during repetitive passive movement improved spinal reciprocal inhibition enhancement and joint movement function
Authors: Hirabayashi Ryo , Edama Mutsuaki , Takeda Mai , Yamada Yuki , Yokota Hirotake , Sekine Chie , Onishi Hideaki
Published: October 2023

This study aimed to evaluate the effects of the participant’s attention target during repetitive passive movement (RPM) intervention on reciprocal inhibition (RI) and joint movement function. Twenty healthy adults participated in two experiments involving four attention conditions [control (forward attention with no RPM), forward attention (during RPM), monitor attention (monitor counting task during RPM), ankle joint attention (ankle movement counting task during RPM)] during 10-min RPM interventions on the ankle joint. Counting tasks were included to ensure the participant’s attention remained on the target during the intervention. In Experiment 1, RI was measured before, immediately after, and 5, 10, 15, 20, and 30 min after the RPM intervention. In Experiment 2, we evaluated ankle joint movement function at the same time points before and after RPM intervention. The maximum ankle dorsiflexion movement (from 30° plantar flexion to 10° dorsiflexion) was measured, reflecting RI. In Experiment 1, the RI function reciprocal Ia inhibition was enhanced for 10 min after RPM under all attention conditions (excluding the control condition. D1 inhibition was enhanced for 20 min after RPM in the forward and monitor attention conditions and 30 min after RPM in the ankle joint attention condition. In Experiment 2, the joint movement function decreased under the forward and monitor attention conditions but improved under the ankle joint attention condition. This study is the first to demonstrate that the participant’s attention target affected the intervention effect of the RI enhancement method, which has implications for improving the intervention effect of rehabilitation.
Put your phone down! Perceived phubbing, life satisfaction, and psychological distress: the mediating role of loneliness
Authors: Maftei Alexandra , Măirean Cornelia
Published: October 2023

The recent literature highlights the need to explore phubbing, a worrying growing phenomenon, and the potential associated factors and outcomes. Thus, the present study focused on perceived phubbing and the potential associations with life satisfaction and psychological distress. Additionally, this study examined the mediating role of loneliness. The cross-sectional, web-based study involved 720 Romanian adults aged 18 to 77 (M = 24.12, SD = 8.80, 74% females) who completed self-reported scales measuring perceived phubbing, and life satisfaction. Results suggested positive associations between perceived phubbing, loneliness, and psychological distress. A significant negative association was also found between loneliness and life satisfaction. Further analyses suggested that loneliness partially mediated the link between perceived phubbing and life satisfaction and between perceived phubbing and psychological distress. The study highlights the significant role of perceived phubbing when discussing psychological distress and life satisfaction, underlining the need to address further the (mis)use of digital devices (e.g., smartphones) within interpersonal relationships, as well as the need to understand better the outcomes of such behaviors to shape effective interventions in this regard.
Dermal effects and pharmacokinetic evaluation of the lidocaine/prilocaine cream in healthy Chinese volunteers
Authors: Li Lingjun , Cai Baole , Li Hongyang , Wei Jun , Tao Lei , Ma Pengcheng
Published: October 2023

Background

EMLA cream is a local anesthetic. The pharmacokinetics and dermal effects of a topical anesthetic formulation has not been evaluated in healthy Chinese volunteers.

Materials and methods

The Pharmacokinetics of the lidocaine/prilocaine test (T) or reference (R, EMLA) cream were evaluated in a fasting, single-dose, two-period crossover bioequivalent study conducted in 40 healthy Chinese volunteers. Meanwhile, the dermal effects including blanching, erythema, temperature sensation, edema, and skin rash were also evaluated during the study.

Results

After applied 15 g of the cream for 4 h to a 100 cm2 area under plastic occlusive film on the skin of the thigh of healthy volunteers, the results of the pharmacokinetic study showed that the active components absorbed in skin from topical products was relatively low compared with most system absorption drugs. After the removal of the residual anesthetic cream, there was a vascular biphasic response with initial transient blanching which reaches a peak at 4.5 h and later more persisting period erythema. The change of temperature sensory sensitivity reached the peak value at 4.5-6 h.There was no statistically significant difference of the changes after application the lidocaine/prilocaine T or R cream in subjects. In general, the lidocaine/prilocaine T or R cream was well tolerated.

Conclusion

The method described a model for investigations of pharmacokinetics and pharmacodynamics of topical lidocaine/prilocaine cream. Except the plasma drug level indicator, these pharmacodynamics data should also be evaluated in the anesthetic transdermal pharmacokinetics study.

Clinical Trial Registration

CTR20211544; registered in http://www.chinadrugtrials.org.cn/ at September 2021.
Investigating the effectiveness of one-class and binary classification for fraud detection
Authors: Leevy Joffrey L. , Hancock John , Khoshgoftaar Taghi M. , Abdollah Zadeh Azadeh
Published: October 2023

Research into machine learning methods for fraud detection is of paramount importance, largely due to the substantial financial implications associated with fraudulent activities. Our investigation is centered around the Credit Card Fraud Dataset and the Medicare Part D dataset, both of which are highly imbalanced. The Credit Card Fraud Detection Dataset is large data and contains actual transactional content, which makes it an ideal benchmark for credit card fraud detection. The Medicare Part D dataset is big data, providing researchers the opportunity to examine national trends and patterns related to prescription drug usage and expenditures. This paper presents a detailed comparison of One-Class Classification (OCC) and binary classification algorithms, utilizing eight distinct classifiers. OCC is a more appealing option, since collecting a second label for binary classification can be very expensive and not possible to obtain within a reasonable time frame. We evaluate our models based on two key metrics: the Area Under the Precision-Recall Curve (AUPRC)) and the Area Under the Receiver Operating Characteristic Curve (AUC). Our results show that binary classification consistently outperforms OCC in detecting fraud within both datasets. In addition, we found that CatBoost is the most performant among the classifiers tested. Moreover, we contribute novel results by being the first to publish a performance comparison of OCC and binary classification specifically for fraud detection in the Credit Card Fraud and Medicare Part D datasets.
Recent advances in nitrogen and nano-nitrogen fertilizers for sustainable crop production: a mini-review
Authors: Verma Krishan K. , Song Xiu-Peng , Degu Hewan Demissie , Guo Dao-Jun , Joshi Abhishek , Huang Hai-Rong , Xu Lin , Singh Munna , Huang Dong-Liang , Rajput Vishnu D. , Li Yang-Rui
Published: October 2023

To meet the global food demand while maintaining the minimum possible negative impacts on the soil, air, and water, sustainable and precise agricultural practices are essential. The efficient use of engineered nanomaterials (ENMs) can replace conventional fertilizers and pesticides, subsequently minimizing the environmental impact of agricultural approaches. Slow-release or controlled-release nitrogenous fertilizers may enhance crop productivity while alleviating agro-environmental constraints. Nitrogen is the essential element which limits worldwide agricultural production. Despite numerous efforts, the N-use efficiency (NUE) in agriculture remains around 50%. The ongoing investigation of novel approaches has resulted in the synthesis of innovative nanomaterials (NMs), providing a potent mechanism for the development of unique element characteristics. The most promising engineered materials being explored, whether for soil or foliar applications, is nanofertilizers. Although not much is known about the usage of NFs, significant results have been observed in various plant species. Granular fertilizers are commonly applied to the soil for the nitrogen requirement of plants. These fertilizers may cause more losses due to the surface runoff or leaching with ammonia volatilization and N oxides (N2O, NO, NOx) emissions. n-NFs are expected to improve NUE by increasing the efficiency of N delivery to plants and minimizing N losses to the environment. A chance to use n-NFs in plants may arise in unique conditions with increasing economic and environmental limitations. This article highlights the possible application of n-NFs as a novel strategy to ensure NUE with the reduction in N losses to the environment, including addressing its potential for sustainable agriculture.

Graphical Abstract
Reproducibility and usefulness of quantitative apparent diffusion coefficient measurements for predicting program death-ligand 1 expression in nasopharyngeal carcinoma
Authors: Zhong Xi , Li Li , Yin Jinxue , Chen Yuanlin , Xin Xin , Yu Lanlan , Tang Yongfang , Zhang Jiangyu , Li Jiansheng
Published: October 2023

Background

Accurate assessment of programmed death-ligand 1 (PD-L1) expression status in nasopharyngeal carcinoma (NPC) before immunotherapy is crucial. We aimed to explore the reproducibility and usefulness of the quantitative apparent diffusion coefficient (ADC) measurements for predicting PD-L1expression status in NPC.

Methods

We retrospectively recruited 134 NPC patients who underwent MRI scans and PD-L1 detection. A PD-L1 combined positive score (CPS) ≥ 20 was identified as high expression status. Patients were divide into two cohorts based on the MRI scanning devices, including a 1.5-T MRI cohort (n = 85, 44 PD-L1 high expression) and a 3.0-T MRI cohort (n = 49, 24 PD-L1 high expression). The mean ADC (ADCmean), minimum ADC (ADCmin) and maximal ADC (ADCmax) values were independently measured by two observers. The ADC measurement reproducibility was assessed by interclass correlation coefficients (ICC). The correlations between ADC parameters and CPS were analyzed by spearman’s correlation coefficient (r), and the performance for PD-L1expression status prediction was assessed by the area under receiver operating characteristic curve (AUC).

Results

The measurement reproducibility of ADCmean, ADCmin and ADCmax was good in the 1.5-T MRI cohort (ICC: 0.843–0.930) and 3.0-T MRI cohort (ICC: 0.929–0.960). The ADCmean, ADCmin, and ADCmax tended to inversely correlate with the CPS (r:-0.37 - -0.52 in the 1.5-T MRI cohort, and − 0.52 - -0.60 in the 3.0-T MRI cohort; P all < 0.01). The ADCmean, ADCmin and ADCmax yielded the AUC of 0.756 (95% CI: 0.651, 0.861), 0.689 (95% CI: 0.576, 0.802), and 0.733 (95%CI: 0.626, 0.839) in the 1.5-T MRI cohort and 0.820 (95%CI: 0.703, 0.937), 0.755 (95% CI: 0.616, 0.894), and 0.760 (95%CI: 0.627, 0.893) in the 3.0-T MRI cohort for predicting PD-L1 high expression status, respectively.

Conclusion

ADC measurements may act as a reproducible and feasible method to predict PD-L1 expression status in NPC.
Preoperative contrast-enhanced CT imaging and clinicopathological characteristics analysis of mismatch repair-deficient colorectal cancer
Authors: Chen Shuai , Du Wenzhe , Cao Yuhai , Kong Jixia , Wang Xin , Wang Yisen , Lu Yang , Li Xiang
Published: October 2023

Background

Colorectal cancer (CRC) can develop through various pathogenetic pathways, and one of the primary pathways is high microsatellite instability (MSI-H)/deficient mismatch repair (dMMR). This study investigated the correlation between preoperative contrast-enhanced CT (CECT) and clinicopathologic characteristics of colorectal cancer (CRC) according to different mismatch repair (MMR) statuses.

Methods

From April 2021 to July 2022, a total of 281 CRC patients with preoperative CECT and available MMR status were enrolled from a single centre for this retrospective study. Preoperative CECT features and clinicopathologic characteristics were analysed. Univariate and multivariate logistic regression analyses were used for statistical analysis. A nomogram was established based on the multivariate logistic regression results. Preoperative and postoperative dynamic nomogram prediction models were established. The C-index, a calibration plot, and clinical applicability of the two models were evaluated, and internal validation was performed using three methods.

Results

In total, 249 patients were enrolled in the proficient mismatch repair (pMMR) group and 32 patients in the deficient mismatch repair (dMMR) group. In multivariate analysis, tumour location (right-hemi colon vs. left-hemi colon, odds ratio (OR) = 2.90, p = .036), the hypoattenuation-within-tumour ratio (HR) (HR > 2/3 vs. HR < 1/3, OR = 36.7, p < .001; HR 1/3–2/3 vs. HR < 1/3, OR = 6.05, p = .031), the number of lymph nodes with long diameter ≥ 8 mm on CECT (OR = 1.32, p = .01), CEA status (CEA positive vs. CEA negative, OR = 0.07, p = .002) and lymph node metastasis (OR = 0.45, p = .008) were independent risk factors for dMMR. Pre- and postoperative C-statistic were 0.861 and 0.908, respectively.

Conclusion

The combination of pre-operative CECT and clinicopathological characteristics of CRC correlates with MMR status, providing possible non-invasive MMR prediction. Particularly for dMMR CRC, tumour-draining lymph node status should be prudently evaluated by CECT.
Standardized protocol of blood pressure measurement and quality control program for the Korea National Health and Nutrition Examination Survey
Authors: Kim Hack-Lyoung , Park Sang Min , Cho In Jeong , Kim Yu-Mi , Kim Dae-Hee , Kim Sung Hye , Kim Kwang-Il , Sung Ki-Chul , Ihm Sang-Hyun , Shin Jinho , Kim Yoonjung , Oh Kyungwon , Lee Eun Mi ,   Quality control team of blood pressure measurements for the Korea National Health, Nutrition Examination Survey (KNHANES)
Published: October 2023

Accurate blood pressure (BP) measurement is crucial for hypertension detection and management. The Korea National Health and Nutrition Examination Survey (KNHANES) assesses the health of Koreans using representative cross-sectional data. BP measurements were historically done with mercury sphygmomanometers for participants aged ≥10 years. However, KNHANES transitioned to Greenlight 300TM (mercury-free auscultatory device) in 2020 for participants aged ≥6 years and used dual devices (Microlife WatchBP Office AFIB and Greenlight) in 2021-2022. To ensure consistency, KNHANES will adopt Microlife as the unified BP device with Greenlight for device validation from 2023. Under the new protocol, participants aged ≥6 years will have their BP measured three times at 30-second intervals after a 5-minute rest under ambient temperature (20-25℃) and noise ≤65 dB. The average of the 2nd and 3rd readings will be used as the representative BP value. The quality control (QC) program involves four trained examiners passing the “quality control and assurance of BP measurement program” three times annually, and undergoing “video monitoring of weekly calibration process” once a year. Additionally, the QC team will conduct “on-site evaluations of BP measurement” at mobile examination centers three times a year. A Five-Step QC process for BP devices was also developed. This document outlines the standardized BP measurement protocol and rigorous QC program in KNHANES, aiming to ensure accurate and reliable BP data for epidemiological research and public health policymaking in South Korea.

Graphical Abstract
The INSCHOOL project: showcasing participatory qualitative methods derived from patient and public involvement and engagement (PPIE) work with young people with long-term health conditions
Authors: Spencer Bethan , Hugh-Jones Siobhan , Cottrell David , Pini Simon
Published: October 2023

Background

Evidence suggests resources and services benefit from being developed in collaboration with the young people they aim to support. Despite this, patient and public involvement and engagement (PPIE) with young people is often tokenistic, limited in engagement and not developmentally tailored to young people. Our paper aims to build knowledge and practice for meaningfully engaging with young people in research design, analysis and as research participants.

Methods

We report the participatory processes from the INSCHOOL project, examining long-term health conditions and schooling among 11–18 year olds. Young people were consulted at the inception of the project through a hospital-based youth forum. This began a partnership where young people co-designed study documents, informed the recruitment process, developed creative approaches to data collection, participated in pilot interviews, co-analysed the qualitative data and co-presented results.

Results

PPIE advisors, participants and researchers all benefitted from consistent involvement of young people throughout the project. Long-term engagement allowed advisors and researchers to build rapport and facilitated openness in sharing perspectives. PPIE advisors valued being able to shape the initial aims and language of the research questions, and contribute to every subsequent stage of the project. Advisors co-designed flexible data collection methods for the qualitative project that provided participants with choices in how they took part (interviews, focus groups, written tasks). Further choice was offered through co-designed preparation activities where participants completed one of four creative activities prior to the interview. Participants were therefore able to have control over how they participated and how they described their school experiences. Through participatory analysis meetings advisors used their first-hand experiences to inform the creation of themes and the language used to describe these themes. PPIE in every stage of the process helped researchers to keep the results grounded in young people’s experience and challenge their assumptions as adults.

Conclusions

Young people have much to offer and the INSCHOOL project has shown that researchers can meaningfully involve young people in all aspects of research. Consistent PPIE resulted in a project where the voices of young people were prioritised throughout and power imbalances were reduced, leading to meaningful participant-centred data.