bluuebunny/crossref_metadata_2025 huggingface dataset:
Artificial neural network model to predict student performance using nonpersonal information
Authors: Chavez Heyul , Chavez-Arias Bill , Contreras-Rosas Sebastian , Alvarez-Rodríguez Jose María , Raymundo Carlos
Published: February 2023

In recent years, artificial intelligence has played an important role in education, wherein one of the most commonly used applications is forecasting students’ academic performance based on personal information such as social status, income, address, etc. This study proposes and develops an artificial neural network model capable of determining whether a student will pass a certain class without using personal or sensitive information that may compromise student privacy. For model training, we used information regarding 32,000 students collected from The Open University of the United Kingdom, such as number of times they took the course, average number of evaluations, course pass rate, average use of virtual materials per date and number of clicks in virtual classrooms. Attributes selected for the model are as follows: 93.81% accuracy, 94.15% precision, 95.13% recall, and 94.64% F1-score. These results will help the student authorities to take measures to avoid withdrawal and underachievement.
The association of cell adhesion molecules and selectins (VCAM-1, ICAM-1, E-selectin, L-selectin, and P-selectin) with microvascular complications in patients with type 2 diabetes: A follow-up study
Authors: Siddiqui Khalid , George Teena P. , Mujammami Muhammad , Isnani Arthur , Alfadda Assim A.
Published: February 2023

Objective

Chronic hyperglycemia induces pathogenic changes in the vascular endothelium and leads to the development of microvascular complications in patients with type 2 diabetes mellitus. Early identification of markers of diabetes complications may help to minimize the risk of the development and progression of microvascular complications.

Methods

This follow-up study was conducted in type 2 diabetic cohort aged between 30-70 years. Out of 160 eligible participants, 70 of them completed follow-up. Levels of cell adhesion molecules and selectins (VCAM-1, ICAM-1, E-selectin, L-selectin and P-selectin) at baseline and follow-up were measured using Randox Evidence biochip analyzer (UK). Development of microvascular complications (diabetic neuropathy, retinopathy and nephropathy) was evaluated.

Results

During the follow-up (2 years, median), 31 (44.3%) developed diabetic neuropathy, 10 (14.3%) developed diabetic retinopathy and, 27 (38.6%) developed diabetic nephropathy. A significant difference in levels of cell adhesion molecules and selectins were found in type 2 diabetic patients with and without microvascular complications. Multiple logistic regression analysis reveals that baseline level of VCAM-1 is significantly associated with microvascular complications; diabetic neuropathy(p=0.028), retinopathy (p=0.007) and nephropathy(p=<0.001). Additionally, levels of P-selectin (p=0.05) and L-selectin (p=0.008) is associated with diabetic nephropathy while retinopathy associated with L-selectin (p=0.005) only.

Conclusion

Cell adhesion molecules and selectins are indicators of microvascular complication among patients with type 2 diabetes (T2D). Association of these markers with the development of microvascular complications may provide additive information for developing strategies for diabetes management and prediction of microvascular complications.
Determinants of poor glycemic control among type 2 diabetes mellitus patients at University of Gondar Comprehensive Specialized Hospital, Northwest Ethiopia: Unmatched case-control study
Authors: Legese Gebrehiwot Lema , Asres Getahun , Alemu Shitaye , Yesuf Tesfaye , Tesfaye Yeabsira Aklilu , Amare Tsegaw
Published: February 2023

Background

Poor glycemic control is one of the most determinant factors for type 2 diabetes-related morbidity and mortality. The proportion of type 2 diabetes mellitus with poor glycemic control remains high. Yet evidences on factors contributing to poor glycemic control remain scarce. The aim of this study is to identify determinants of poor glycemic control among type 2 diabetes mellitus patients at a diabetes mellitus clinic in University of Gondar Comprehensive Specialized Hospital, Northwest Ethiopia.

Methods

A hospital-based case-control study was conducted from June to September 2020. Using convenience sampling techniques, a total of 90 cases and 90 controls with type 2 diabetes were recruited. The data were entered into Epidata version 4.6.0.2 and analyzed by Stata version 14. A multivariable logistic regression analysis was performed to assess the association between independent variables and glycemic control. Both 95% CI and p-value<0.05 were used to determine the level and significance, respectively.

Results

The mean age ( ± standard deviations) for the cases and controls were 57.55± 10.42 and 61.03± 8.93% respectively. The determinants of poor glycemic control were age (Adjusted odd ratio (AOR)= 0.08; 95% CI= 0.02-0.33), inadequate physical exercise (AOR = 5.05; 95% CI = 1.99-11.98), presence of comorbidities (AOR = 5.50; 95% CI = 2.06-14.66), non-adherence to anti-diabetes medications (AOR= 2.76; 95% CI= 1.19-6.40), persistent proteinuria (AOR=4.95; 95% CI=1.83-13.36) and high-density lipoprotein less than 40 mg/dl (AOR=3.08; 95% CI= 1.30-7.31).

Conclusions

Age less than 65 years, inadequate physical exercise, presence of comorbidities, non-adherence to anti-diabetes medications, persistent proteinuria, and high-density lipoprotein less than 40 mg/dl were the determinants of poor glycemic control. Therefore, targeted educational and behavioral modification programs on adequate exercise and medication adherence should be routinely practiced. Furthermore, early guideline-based screening and treatment of comorbidities and complications is required to effectively manage diabetes mellitus.
Visceral fat and attribute-based medicine in chronic kidney disease
Authors: Kataoka Hiroshi , Nitta Kosaku , Hoshino Junichi
Published: February 2023

Visceral adipose tissue plays a central role in obesity and metabolic syndrome and is an independent risk factor for both cardiovascular and metabolic disorders. Increased visceral adipose tissue promotes adipokine dysregulation and insulin resistance, leading to several health issues, including systemic inflammation, oxidative stress, and activation of the renin-angiotensin-aldosterone system. Moreover, an increase in adipose tissue directly and indirectly affects the kidneys by increasing renal sodium reabsorption, causing glomerular hyperfiltration and hypertrophy, which leads to increased proteinuria and kidney fibrosis/dysfunction. Although the interest in the adverse effects of obesity on renal diseases has grown exponentially in recent years, the relationship between obesity and renal prognosis remains controversial. This may be attributed to the long clinical course of obesity, numerous obesity-related metabolic complications, and patients’ attributes. Multiple individual attributes influencing the pathophysiology of fat accumulation make it difficult to understand obesity. In such cases, it may be effective to elucidate the pathophysiology by conducting research tailored to individual attributes from the perspective of attribute-based medicine/personalized medicine. We consider the appropriate use of clinical indicators necessary, according to attributes such as chronic kidney disease stage, level of visceral adipose tissue accumulation, age, and sex. Selecting treatments and clinical indicators based on individual attributes will allow for advancements in the clinical management of patients with obesity and chronic kidney disease. In the clinical setting of obesity-related nephropathy, it is first necessary to accumulate attribute-based studies resulting from the accurate evaluation of visceral fat accumulation to establish evidence for promoting personalized medicine.
Cyber-physical cascading failure and resilience of power grid: A comprehensive review
Authors: Islam Md Zahidul , Lin Yuzhang , Vokkarane Vinod M. , Venkataramanan Venkatesh
Published: February 2023

Smart grid technologies are based on the integration of the cyber network and the power grid into a cyber-physical power system (CPPS). The increasing cyber-physical interdependencies bring about tremendous opportunities for the modeling, monitoring, control, and protection of power grids, but also create new types of vulnerabilities and failure mechanisms threatening the reliability and resiliency of system operation. A major concern regarding the interdependent networks is the cascading failure (CF), where a small initial disturbance/failure in the network results in a seemingly unexpected large-scale failure. Although there has been a significant volume of recent work in the CF research of CPPS, a comprehensive review remains unavailable. This article aims to fill the gap by providing a systematic literature survey regarding the modeling, analysis, and mitigation of CF in CPPS. The open research questions for further research are also discussed. This article allows researchers to easily understand the state of the art of CF research in CPPS and fosters future work required towards full resolutions to the remaining questions and challenges.
Impacts of urbanization, LULC, LST, and NDVI changes on the static water table with possible solutions and water policy discussions: A case from Islamabad, Pakistan
Authors: Sohail Muhammad Tayyab , Manzoor Zaira , Ehsan Muhsan , Al-Ansari Nadhir , Khan Muhammad Bashir , Shafi Ahsan , Ullah Jar , Hussain Asrar , Raza Danish , Usman Umar , Akbar Sameem , Elbeltagi Ahmed
Published: February 2023

Rapid urbanization, coupled with land use land cover changes (LULC), has caused stress on freshwater resources around the globe. As in the case of Islamabad, the capital of Pakistan, the population has increased significantly, creating a deficit of natural resources and affecting the environment adversely. Therefore, the purpose of this study is to examine the effects of urbanization and LULC on the decline of the static water table in Islamabad. It also seeks to analyze water policy issues in order to achieve sustainable water resource development. The excessive pumping of the existing groundwater has exceeded the safe limit, which is justified by the constantly growing population. However, the changes in the LULC of the study area have turned many green pastures into barren land. Our research data were obtained from the Capital Development Authority (CDA), Pakistan Meteorological Department (PMD), and Landsat Satellite images. After analyzing PMD and CDA data for the last 20 years (2000–2020), the results were interpreted using Arc GIS. It has been observed that the Normalized Difference Vegetation Index (NDVI) value increases as the Land Surface Temperature (LST) decreases. Therefore, the overall observation is a decreasing trend in Islamabad temperatures due to the increased vegetation in the study area during the period of 2000–2020. It was observed that there has been a considerable drop in water levels due to over-pumping in a few areas. It is primarily associated with the increasing population of the capital in the last 2 decades. This study uses a survey to explore the potential locations for check dams to enhance and recharge the groundwater aquifers in the capital, Islamabad. It suggests catchment areas throughout the Margalla Hills along with different localities, such as Rumli Village, Trail 5, and Shahdara.
The product space and evolution of international competitiveness—evidence from China’s strategic and critical mineral articles
Authors: Zhu Wenxing , Hu Tingting , Xiao Wei , Liao Jinping , Xu Yue
Published: February 2023

Clarifying the evolution of international competitiveness for strategic and critical mineral articles is of great significance for promoting new energy development and achieving carbon neutrality in China. This study, therefore, calculates the series of indicators based on product space theory, for instance, international import & export shares, revealed comparative advantage (RCA) and product density, to analyze the competitiveness evolution. Results show that: the international competitiveness of China’s strategic and critical mineral articles demonstrated a declining tendency, and the rising percentage of imports was much larger than the decreasing export percentage. The evolution of the international competitiveness of 11 strategic and critical minerals is different. Among them, the international competitiveness of two articles is very strong, that of four articles is relatively weak, and that of three products is decreasing trend. From the perspectives of industrial sectors and trade, we analyze the underlying reasons for the competitiveness evolution of 11 strategic and critical mineral articles in detail and discuss the industry transformation and upgrade feasibility for strategic and critical mineral articles. Our work aims to provide strong support for the upgrading and high-quality development of the strategic and critical mineral products industry by providing corresponding policy suggestions.
Evaluation of heavy metal contamination of soil and the health risks in four potato-producing areas
Authors: Zhang Jie , Liu Ke , He Xue , Li Wei , Zhang Meng , Cai Quan
Published: February 2023

Areas polluted by heavy metals in soil may pose a major risk to human health and ecological environment safety. In this study, 89 soil samples were collected from four potato producing areas (Libo, Chishui, Panxian and Weining) in Guizhou Province, China, and the concentrations of 9 soil heavy metals were analyzed and measured. The aims of this study was to evaluate the human health risk and pollution index of heavy metals in the soil of some potato-producing areas in Guizhou Province by using the geoaccumulation index method, pollution load index method and health risk assessment method. The results revealed that Igeo<0 in Libo and Chishui, Igeo>0 in Panxian except Pb, The Igeo of As and Sb were less than 0 in Weining, and other elements were polluted to varying degrees. The pollution load index is Panxian (1.47) > Weining (1.39) > Libo (0.67), Chishui (0.67), Libo and Chishui were generally no polluted, soils in Panxian and Weining were polluted. The health risks of potatoes through food ingestion are less than 1. In terms of carcinogenesis, the risk of human in each study area through the ingestion was Cr > As > Cd. Cr and As would be produced certain carcinogenic risk to human through the dermal contact. Cr had a strong carcinogenic risk to adults through the inhalation. In terms of non-carcinogenesis, children in each study area had a strong risk under the each pathways. The risk of carcinogenesis in adults through inhalation pathway is greater than that in children, and the risk of carcinogenesis and non-carcinogenesis in children through Ingestion and dermal contact pathway is greater than adults. The results of this study suggest that attention should be paid to the remediation of heavy metals in contaminated soil to protect human health.
Bioeconomy-based tourism: A new concept responding to the support of bioeconomy
Authors: Rinn Radek , Kalábová Markéta , Jarský Vilém
Published: February 2023

Tourism has experienced dynamic development in recent decades and has become one of the fastest growing industries; however, the COVID-19 pandemic caused unprecedented changes and declines in revenues and tourism trends. With the strong support of bioeconomy (BE) this period brings a great opportunity to transform the entire tourism sector into a model responding to that concept. The main aim of this article is to prove whether and under what possible conditions tourism is a part of the concept of BE. Selected European Union (EU) countries have been chosen so that every geographical area is represented. This analysis was therefore carried out for a total of 12 states and the EU as a whole (13 entities). Of these 13 entities, seven have their own BE strategy and six do not. According to the level of emphasis on the field of tourism in the examined documents, we divided the states into three categories. Although the selected and analysed countries mention tourism in their BE strategies with various emphasis and approach, the inclusion of tourism under this concept is evident. Bioeconomy-based tourism has been designed as a new concept that considers the approach of BE. It focuses above all on a sustainable level of tourism in the connection of economic and environmental pillars, where the main emphasis is placed on the effective use of natural resources and maximum effort not to waste these resources, as well as to support economic growth.
Knowing but not doing: Quantifying the research-implementation gap in conservation paleobiology
Authors: Groff Dulcinea V. , McDonough MacKenzie Caitlin , Pier Jaleigh Q. , Shaffer Austin B. , Dietl Gregory P.
Published: February 2023

Conservation paleobiology aims to provide a longer-term perspective on environmental problems to inform decisions about natural resource conservation. As such, conservation paleobiology research falls short when geohistorical data and insights do not inform conservation practice, contributing to the well-known idea that a “gap” exists between the production and use of science in the environmental realm. Our study quantified the extent of this research-implementation (or knowing-doing) gap through a systematic literature review and survey questionnaire. We determined whether empirical studies in conservation paleobiology with a link to conservation, management, or restoration documented the use of geohistorical data to implement some form of action or if there was a specific mention of how the geohistorical data could be used in theory. Results indicate that “applied” conservation paleobiology has a poor record of translating research into action. Tangible conservation impacts were evident in only 10.8% of studies. Over half of these studies included coauthors affiliated with a conservation organization. Among the studies coded as having a theoretical application, 25.2% specified how the geohistorical data could be implemented to enhance conservation, management, or restoration actions. All studies documenting action used geohistorical data from the geologically recent past where the species and habitats are familiar to those found today. Drawing insights from the bright spots we identified, we offer some practical suggestions to narrow the gap between knowing and doing in conservation paleobiology.
Genetic variants in BAT2 are associated with immune responsiveness to influenza vaccination
Authors: Wen Simin , Wei Hejiang , Li Mao , Zhong Shuyi , Cheng Yanhui , Huang Weijuan , Wang Dayan , Shu Yuelong
Published: February 2023

Background: Influenza is a global public health problem for its detrimental impact on human health. Annual vaccination is the most effective prevention of influenza infection. Identifying host genetic factors associated with the responsiveness to influenza vaccines can provide clues for developing more effective influenza vaccines. In this study, we aimed to explore whether the single nucleotide polymorphisms in BAT2 are associated with the antibody responses to influenza vaccines.

Method: A nested case-control study was conducted in this research. 1968 healthy volunteers were enrolled and 1,582 of them from a Chinese Han population were eligible for further research. According to the hemagglutination inhibition titers of subjects against all influenza vaccine strains, a total of 227 low responders and 365 responders were included in the analysis. Six tag single nucleotide polymorphisms in the coding region of BAT2 were selected and genotyped using the MassARRAY technology platform. Univariable and multivariable analyses were conducted to evaluate the relationship between variants and antibody responses to influenza vaccination.

Results: Multivariable logistic regression analysis showed that, compared with the BAT2 rs1046089GG genotype, the GA + AA genotype was correlated with decreased risk of low responsiveness to influenza vaccines after adjusting for gender and age (p = 1.12E-03, OR = .562, 95%CI: .398–.795). rs9366785 GA + AA genotype was associated with a higher risk of low responsiveness to influenza vaccination compared with the GG genotype (p = .003, OR = 1.854, 95%CI: 1.229–2.799). The haplotype consisting of BAT2 rs2280801-rs10885-rs1046089-rs2736158-rs1046080-rs9366785 CCAGAG was correlated with a higher level of antibody response to influenza vaccines compared with haplotype CCGGAG (p < .001, OR = .37, 95%CI: .23–.58).

Conclusion: Genetic variants in BAT2 were statistically associated with the immune response to influenza vaccination among the Chinese population. Identifying these variants will provide clues for further research on novel broad-spectrum influenza vaccines, and improve the individualized influenza vaccination scheme.
Identification and validation of hub genes and potential drugs involved in osteoarthritis through bioinformatics analysis
Authors: Xu Wenbo , Wang Xuyao , Liu Donghui , Lin Xin , Wang Bo , Xi Chunyang , Kong Pengyu , Yan Jinglong
Published: February 2023

Purpose: Osteoarthritis (OA) is a common degenerative disease, which still lacks specific therapeutic drugs. Synovitis is one of the most important pathological process in OA. Therefore, we aim to identify and analyze the hub genes and their related networks of OA synovium with bioinformatics tools to provide theoretical basis for potential drugs.

Materials and methods: Two datasets were obtained from GEO. DEGs and hub genes of OA synovial tissue were screened through Gene Ontology (GO) annotation, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment as well as protein—protein interaction (PPI) network analysis. Subsequently, the correlation between expression of hub genes and ferroptosis or pyroptosis was analyzed. CeRNA regulatory network was constructed after predicting the upstream miRNAs and lncRNAs. The validation of hub genes was undertook through RT-qPCR and ELISA. Finally, potential drugs targeting pathways and hub genes were identified, followed by the validation of the effect of two potential drugs on OA.

Results: A total of 161 commom DEGs were obtained, of which 8 genes were finally identified as hub genes through GO and KEGG enrichment analysis as well as PPI network analysis. Eight genes related to ferroptosis and pyroptosis respectively were significantly correlated to the expression of hub genes. 24 miRNAs and 69 lncRNAs were identified to construct the ceRNA regulatory network. The validation of EGR1, JUN, MYC, FOSL1, and FOSL2 met the trend of bioinformatics analysis. Etanercept and Iguratimod reduced the secretion of MMP-13 and ADAMTS5 of fibroblast-like synoviocyte.

Conclusion: EGR1, JUN, MYC, FOSL1, and FOSL2 were identified as hub genes in the development of OA after series of bioinformatics analysis and validation. Etanercept and Iguratimod seemed to have opportunities to be novel drugs for OA.
Dysregulation of innate cell types in the hepatic immune microenvironment of alcoholic liver cirrhosis
Authors: Ren Ao , He Wenjing , Rao Jiawei , Ye Dongmei , Cheng Pengrui , Jian Qian , Fu Zongli , Zhang Xuzhi , Deng Ronghai , Gao Yifang , Ma Yi
Published: February 2023

Introduction

The risk of alcoholic cirrhosis increases in a dose- and time-dependent manner with alcohol consumption and ethanol metabolism in the liver. Currently, no effective antifibrotic therapies are available. We aimed to obtain a better understanding of the cellular and molecular mechanisms involved in the pathogenesis of liver cirrhosis.

Methods

We performed single-cell RNA-sequencing to analyze immune cells from the liver tissue and peripheral blood form patients with alcoholic cirrhosis and healthy controls to profile the transcriptomes of more than 100,000 single human cells and yield molecular definitions for non-parenchymal cell types. In addition, we performed single-cell RNA-sequencing analysis to reveal the immune microenvironment related to alcoholic liver cirrhosis. Hematoxylin and eosin, Immunofluorescence staining and Flow cytometric analysis were employed to study the difference between tissues and cells with or without alcoholic cirrhosis.

Results

We identified a fibrosis-associated M1 subpopulation of macrophages that expands in liver fibrosis, differentiates from circulating monocytes, and is pro-fibrogenic. We also define mucosal-associated invariant T (MAIT) cells that expand in alcoholic cirrhosis and are topographically restricted to the fibrotic niche. Multilineage modeling of ligand and receptor interactions between the fibrosis-associated macrophages, MAIT, and NK cells revealed the intra-fibrotic activity of several pro-fibrogenic pathways, including responses to cytokines and antigen processing and presentation, natural killer cell-mediated cytotoxicity, cell adhesion molecules, Th1/Th2/Th17 cell differentiation, IL-17 signaling pathway, and Toll-like receptor signaling pathway.

Discussion

Our work dissects unanticipated aspects of the cellular and molecular basis of human organ alcoholic fibrosis at the single-cell level and provides a conceptual framework for the discovery of rational therapeutic targets in liver alcoholic cirrhosis.
Targeting β-glucans, vital components of the Pneumocystis cell wall
Authors: Wang Mengyan , Zhang Zhongdong , Dong Xiaotian , Zhu Biao
Published: February 2023

β-glucan is the most abundant polysaccharide in the cell wall of Pneumocystis jirovecii, which has attracted extensive attention because of its unique immunobiological characteristics. β-glucan binds to various cell surface receptors, which produces an inflammatory response and accounts for its immune effects. A deeper comprehension of the processes by Pneumocystis β-glucan recognizes its receptors, activates related signaling pathways, and regulates immunity as required. Such understanding will provide a basis for developing new therapies against Pneumocystis. Herein, we briefly review the structural composition of β-glucans as a vital component of the Pneumocystis cell wall, the host immunity mediated by β-glucans after their recognition, and discuss opportunities for the development of new strategies to combat Pneumocystis.
An ex vivo test to investigate genetic factors conferring susceptibility to atypical haemolytic uremic syndrome
Authors: Gastoldi Sara , Aiello Sistiana , Galbusera Miriam , Breno Matteo , Alberti Marta , Bresin Elena , Mele Caterina , Piras Rossella , Liguori Lucia , Santarsiero Donata , Benigni Ariela , Remuzzi Giuseppe , Noris Marina
Published: February 2023

Introduction

Comprehensive genetic analysis is essential to clinical care of patients with atypical haemolytic uremic syndrome (aHUS) to reinforce diagnosis, and to guide treatment. However, the characterization of complement gene variants remains challenging owing to the complexity of functional studies with mutant proteins. This study was designed: 1) To identify a tool for rapid functional determination of complement gene variants; 2) To uncover inherited complement dysregulation in aHUS patients who do not carry identified gene variants.

Methods

To address the above goals, we employed an ex-vivo assay of serum-induced C5b-9 formation on ADP-activated endothelial cells in 223 subjects from 60 aHUS pedigrees (66 patients and 157 unaffected relatives).

Results

Sera taken from all aHUS patients in remission induced more C5b-9 deposition than control sera, independently from the presence of complement gene abnormalities. To avoid the possible confounding effects of chronic complement dysregulation related to aHUS status, and considering the incomplete penetrance for all aHUS-associated genes, we used serum from unaffected relatives. In control studies, 92.7% of unaffected relatives with known pathogenic variants exhibited positive serum-induced C5b-9 formation test, documenting a high sensitivity of the assay to identify functional variants. The test was also specific, indeed it was negative in all non-carrier relatives and in relatives with variants non-segregating with aHUS. All but one variants in aHUS-associated genes predicted in-silico as likely pathogenic or of uncertain significance (VUS) or likely benign resulted as pathogenic in the C5b-9 assay. At variance, variants in putative candidate genes did not exhibit a functional effect, with the exception of a CFHR5 variant. The C5b-9 assay in relatives was helpful in defining the relative functional effect of rare variants in 6 pedigrees in which the proband carried more than one genetic abnormality. Finally, for 12 patients without identified rare variants, the C5b-9 test in parents unmasked a genetic liability inherited from an unaffected parent.

Discussion

In conclusion, the serum-induced C5b-9 formation test in unaffected relatives of aHUS patients may be a tool for rapid functional evaluation of rare complement gene variants. When combined with exome sequencing the assay might be of help in variant selection, to identify new aHUS-associated genetic factors.
Early immune system alterations in patients with septic shock
Authors: Tang Huiming , Qin Shuang , Li Zhanfei , Gao Wei , Tang Manli , Dong Xijie
Published: February 2023

This study aims to investigate the early changes in the immune systems of patients with septic shock. A total of 243 patients with septic shock were included in this study. The patients were classified as survivors (n = 101) or nonsurvivors (n = 142). Clinical laboratories perform tests of the immune system’s function. Each indicator was studied alongside healthy controls (n = 20) of the same age and gender as the patients. A comparative analysis of every two groups was conducted. Univariate and multivariate logistic regression analyses were performed to identify mortality risk factors that are independent of one another. In septic shock patients, neutrophil counts, infection biomarkers (C-reactive protein, ferritin, and procalcitonin levels), and cytokines (IL-1β, IL-2R, IL-6, IL-8, IL-10, and TNF-α) increased significantly. Lymphocyte and their subset counts (T, CD4+ T, CD8+ T, B, and natural killer cell counts), lymphocyte subset functions (the proportions of PMA/ionomycin-stimulated IFN-γ positive cells in CD4+ T cells), immunoglobulin levels (IgA, IgG, and IgM), and complement protein levels (C3 and C4) decreased significantly. Compared to survivors, nonsurvivors had higher levels of cytokines (IL-6, IL-8, and IL-10) but lower levels of IgM, complement C3 and C4, and lymphocyte, CD4+, and CD8+ T cell counts. Low IgM or C3 concentrations and low lymphocyte or CD4+ T cell counts were independent risk factors for mortality. These alterations should be considered in the future development of immunotherapies aimed at treating septic shock.
Subsurface dispersion path and travel time of radiocesium from Fukushima by Mode Water
Authors: Lee Seung-Tae , Jung Jihun , Kim Guebuem , Tak Yong-Jin , Cho Yang-Ki
Published: February 2023

The Fukushima Daiichi Nuclear Power Plant (FDNPP) accident on March 11, 2011 resulted in the release of immense amounts of radioactive materials into the ocean. However, the dispersion of radioactive materials in the subsurface has not yet been clarified due to the spatiotemporal limitation of observations. Thus, herein, a tracer experiment was implemented using a three-dimensional numerical model to estimate the dispersion path of 137Cs released directly from the FDNPP and its travel time in the subsurface of the North Pacific Ocean from 2011 to 2020. The results show that the subsurface 137Cs spreads by two mode waters, namely, Sub-Tropical Mode Water (STMW) and Central Mode Water (CMW). Subsurface 137Cs primarily spreads clockwise in the sub-tropical region, while a portion driven by STMW is dispersed southward. The clockwise dispersion path of 137Cs released into the ocean by STMW is relatively shallower and inward than that by CMW. The 137Cs that was spread clockwise reached the east of Taiwan and the Philippines via STMW and CMW nine years after the accident, respectively. The model described in this study is applicable for estimating the water path and travel time of tritium water planned to be discharged from Fukushima.
Hydroacoustic survey on fish spatial distribution in the early impoundment stage of Yuwanghe Reservoir in southwest China
Authors: Luo Bin , Zhou Xianjun , Zhang Chaoshuo , Bao Jianghui , Mei Feng , Lian Yuxi , Zhang Dongxu , Hu Shaoqiu , Guo Longgen , Duan Ming
Published: February 2023

Understanding the fish community structure and spatial distribution characteristics is essential for appropriate reservoir fishery management, especially in the early impoundment stage of the reservoir, which could help in obtaining basic data and performing artificial adjustments to biological communities. On the basis of this concept, we conducted a survey of the fish community and distribution at the newly constructed reservoir in southwest China by using a combination of methods, including hydroacoustic survey and fish sampling. Fish sampling showed a single fish community structure (six species belonging to four families) assembled in the reservoir, and the dominant species was Pseudorasbora parva which accounted for 98.79% of the fish population. In the hydroacoustic survey, the average fish density was 318.7 ± 256.1 individuals/1,000 m2. Irregular distribution of the fish was observed in the horizontal direction. The fish densities in the head area, middle area, and tail area of the reservoir were 168.5 ± 60.1, 306.8 ± 124.7, and 696.4 ± 288.9 individuals/1,000 m2, respectively, which showed a trend of increase in fish density with an increase in distance from the dam. More than 97.3% of the fish in the vertical direction were distributed in the water layer of depth 0 to 12 m. The average total length of fish was 75.4 mm, and the mean fish biomass in the reservoir was approximately 984.8 g/1,000 m2. According to Pearson correlation analysis, the main factors affecting the spatial distribution of fish were water depth, water temperature, dissolved oxygen, total nitrogen, and plankton density. Our results suggested that the fish community structure should be adjusted by releasing native carnivorous fish to control the abundance of small fish. Moreover, filter-feeding fish such as silver carp and bighead carp should be released in the reservoir to control the plankton community; this will enable the maintenance of the reservoir ecosystem in a healthy state while increasing the economic benefits to the local area.
Seasonal variability of nektonic community structure and phylogenetic diversity in Weizhou Island, the Beibu Gulf
Authors: Xu Lei , Du Feiyan , Tang Quehui , Wang Lianggen , Ning Jiajia , Huang Delian , Li Yafang , Liu Shuangshuang , Wang Xuehui
Published: February 2023

The favorable natural conditions and variety of habitats in the Beibu Gulf provide a basis for harboring a high diversity of marine organisms. Sustainable coastal ecosystem management can be benefited from a comprehensive assessment of species diversity. In this study, we analyzed the seasonal changes in nektonic phylogenetic and community structures in the waters of Weizhou Island in the northern Beibu Gulf. The results showed that both the nektonic phylogenetic diversity and community structure in the northern Beibu Gulf exhibited strong seasonal differences between spring and autumn. The catch density was 291.9 kg per km2 in spring and 1081.1 kg per km2 in autumn. Phylogenetic diversity of nektonic communities obviously increased from spring to autumn, while phylogenetic patterns changed from clustering to overdispersion. The seasonal patterns of nektonic communities were mainly related to the different fishing intensities in spring and autumn. Summer fishing intensity in the Beibu Gulf was effectively controlled by a mid-summer fishing moratorium, during which nektonic diversity and fish stocks rapidly recovered from the larval pool. Our study revealed that fishing intensity had a greater impact on nektonic communities at smaller spatial scales, and even exceeded the effects of environmental factors.
Recent advances in interface microscopic characterization of carbon fiber-reinforced polymer composites
Authors: Liu Liting , Du Minshu , Liu Feng
Published: February 2023

Carbon fiber reinforced polymer composites (CFRPs) have excellent properties, e.g., low density, high-temperature resistance, high specific modulus, and high specific strength, and are widely used in aerospace and civil industries. CFRP comprises carbon fiber, polymer matrix, and the interface between them. The microstructure, chemical composition, bonding mode, and interfacial bonding strength of the CFRPs interface greatly influence the mechanical properties and failure behavior of CFRPs. Accordingly, the deep, systematic and nuanced characterization of the microstructure and properties of the interfaces is one of the critical problems in the research field of CFRPs. In this paper, various microscopic characterization methods of CFRPs interface micromorphology, microstructure, chemical composition and mechanical properties developed in recent years are reviewed. For example, scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy (Raman), nanoindentation and other advanced analytical characterization techniques, as well as the application of newly developed microscopic in situ mechanical testing methods in the interface characterization of CFRPs. The prospect and trend of interface microscopic characterization technology of CFRPs have also been prospected.
Safety and efficacy of 650 nm invasive laser acupuncture on non-specific chronic low back pain: A protocol for a multicenter randomized placebo-controlled trial
Authors: Kim Jae-Hong , Yang Changsop , Yoo Jaehee , Park Gwang-Cheon , Kang Byoung-Kab , Kim Ae-Ran , Kim Jihye , Nam Dongwoo , Hong Yejin
Published: February 2023

Background

We aim to obtain clinical trial data regarding the safety, efficacy, and usefulness of invasive laser acupuncture (ILA) for non-specific chronic low back pain (NSCLBP) through a randomized placebo-controlled trial.

Methods

Our clinical trial will be an assessor- and patient-blinded, prospective, parallel-arm, multi-center, randomized placebo-controlled clinical trial. One hundred and six participants with NSCLBP will be allocated evenly to the 650 ILA or control group. All participants will receive education on exercise and self-management. The 650 ILA group will undergo 650 nm ILA for 10 min, and the control group will undergo sham ILA for 10 min per visit, twice a week for 4 weeks, at bilateral GB30, BL23, BL24, and BL25. The primary outcome will be the proportion of responders (≥30% reduction in pain visual analogue scale [VAS] without increased use of painkillers) at 3 days after the intervention ends. The secondary outcomes will include changes in the scores of the VAS, European Quality of Life Five Dimension Five Level scale, and Korean version of the Oswestry Disability Index at 3 days after the intervention ends and 8 weeks after the intervention ends.

Discussions

The results of our study will provide clinical evidence concerning the safety and efficacy of 650 nm ILA for the management of NSCLBP.

Clinical trial registration

https://cris.nih.go.kr/cris/search/detailSearch.do?search_lang=E&focus=reset_12&search_page=M&pageSize=10&page=undefined&seq=21591&status=5&seq_group=21591, identifier KCT0007167.
Diagnosis and treatment of infectious vaginitis: Proposal for a new algorithm
Authors: Eleutério José , Campaner Adriana Bittencourt , de Carvalho Newton Sergio
Published: February 2023

Background

Vaginitis is the most common gynecologic diagnosis in primary care, and most women have at least one episode during their lives. The need for standardized strategies to diagnose and treat vaginitis, both in primary care and among gynecologists, is emphasized. The Brazilian Group for Vaginal Infections (GBIV, acronym in Portuguese) aimed to update the practical approach to affected women by reviewing and discussing recent literature, and developing algorithms for diagnosis and treatment of vaginitis.

Methods

A literature search within biomedical databases PubMed and SCieLo was conducted in January 2022. The available literature was evaluated by three experienced researchers, members of the GBIV, to summarize the main data and develop practical algorithms.

Results and conclusion

Detailed algorithms were developed with the main goal to improve gynecological practice considering different scenarios and access to diagnostic tools, from the simplest to the most complex tests. Different age groups and specific contexts were also considered. The combination of anamnesis, gynecological examination, and complementary tests remains the basis of a proper diagnostic and therapeutic approach. Periodic updates of these algorithms are warranted as new evidence becomes available.
The accelerated waning of immunity and reduced effect of booster in patients treated with bDMARD and tsDMARD after SARS-CoV-2 mRNA vaccination
Authors: Tobudic Selma , Simader Elisabeth , Deimel Thomas , Straub Jennifer , Kartnig Felix , Heinz Leonhard X. , Mandl Peter , Haslacher Helmuth , Perkmann Thomas , Schneider Lisa , Nothnagl Thomas , Radner Helga , Winkler Florian , Burgmann Heinz , Stiasny Karin , Novacek Gottfried , Reinisch Walter , Aletaha Daniel , Winkler Stefan , Blüml Stephan
Published: February 2023

Objectives

This study aimed to assess the duration of humoral responses after two doses of SARS-CoV-2 mRNA vaccines in patients with inflammatory joint diseases and IBD and booster vaccination compared with healthy controls. It also aimed to analyze factors influencing the quantity and quality of the immune response.

Methods

We enrolled 41 patients with rheumatoid arthritis (RA), 35 with seronegative spondyloarthritis (SpA), and 41 suffering from inflammatory bowel disease (IBD), excluding those receiving B-cell-depleting therapies. We assessed total anti-SARS-CoV-2 spike antibodies (Abs) and neutralizing Ab titers 6 months after two and then after three doses of mRNA vaccines compared with healthy controls. We analyzed the influence of therapies on the humoral response.

Results

Patients receiving biological or targeted synthetic disease-modifying antirheumatic drugs (b/tsDMARDs) showed reduced anti-SARS-CoV-2 S Abs and neutralizing Ab titers compared with HC or patients receiving conventional synthetic (cs)DMARDs 6 months after the first two vaccination doses. Anti-SARS-CoV-2 S titers of patients with b/tsDMARDs declined more rapidly, leading to a significant reduction in the duration of vaccination-induced immunity after two doses of SARS-CoV-2 mRNA vaccines. While 23% of HC and 19% of patients receiving csDMARDs were without detectable neutralizing Abs 6 months after the first two vaccination doses, this number was 62% in patients receiving b/tsDMARDs and 52% in patients receiving a combination of csDMARDs and b/tsDMARDs. Booster vaccination led to increased anti-SARS-CoV-2 S Abs in all HC and patients. However, anti-SARS-CoV-2 S Abs after booster vaccination was diminished in patients receiving b/tsDMARDs, either alone or in combination with csDMARDs compared to HC.

Conclusion

Patients receiving b/tsDMARDs have significantly reduced Abs and neutralizing Ab titers 6 months after mRNA vaccination against SARS-CoV-2. This was due to a faster decline in Ab levels, indicating a significantly reduced duration of vaccination-induced immunity compared with HC or patients receiving csDMARDs. In addition, they display a reduced response to a booster vaccination, warranting earlier booster vaccination strategies in patients under b/tsDMARD therapy, according to their specific Ab levels.
Determinants of patient and physician global assessments of disease activity in anti-neutrophil cytoplasmic antibody-associated vasculitis
Authors: Rohde Marius , Kernder Anna , Acar Hasan , Düsing Christina , Fischer-Betz Rebecca , Haase Isabell , Mucke Johanna , Sander Oliver , Richter Jutta , Filla Tim , Schneider Matthias , Chehab Gamal
Published: February 2023

Objective

To compare physician and patient assessments of global disease activity in anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) and to identify associated factors.

Methods

Global disease activity scores (0–10 points) were retrospectively analyzed from physicians and patients with AAV at each outpatient visit from 2010 to 2020. We compared the scores and performed a linear regression with a random effects to identify associated factors.

Results

Patients (n = 143, 1,291 pairs, 52% female) had a mean 64 (±15) years of age and a mean disease duration of 9 (±7) years. Patients and physicians global disease activity assessments showed a moderate correlation (Pearson R 0.31, CI [0.23–0.52], p < 0.001). Linear regression showed a strong association between the physician-documented disease activity scores and serum CRP levels (β = 0.22, CI [0.18, 0.28]), disease duration (β = −0.022, CI [−0.04,-0.01]) and patients’ assessment of disease activity (β = 0.08, CI [0.04, 0.12]). By contrast, patient assessments were strongly associated with the degree of pain (β = 0.30, CI [0.25, 0.35]), functional limitations in daily living (HAQ, β = 0.49, CI [0.21, 0.78]) and the global physical well-being (NRS, β = 0.39, CI [0.32, 0.46]).

Conclusion

Patients’ and physicians’ assessments of disease activity correlated. High CRP levels and disease duration were associated with physician-assessed disease activity scores, while subjective limitations were associated with higher patient-assessed disease activity scores. These findings highlight and support the need to develop and evaluate patient-reported outcomes to assess disease activity in patients diagnosed with AAV.
Expanding the diversity of Chardonnay aroma through the metabolic interactions of Saccharomyces cerevisiae cocultures
Authors: Bordet Fanny , Romanet Rémy , Bahut Florian , Ballester Jordi , Eicher Camille , Peña Cristina , Ferreira Vicente , Gougeon Régis , Julien-Ortiz Anne , Roullier-Gall Chloé , Alexandre Hervé
Published: February 2023

Yeast co-inoculations in winemaking are often studied in the framework of modulating the aromatic profiles of wines. Our study aimed to investigate the impact of three cocultures and corresponding pure cultures ofSaccharomyces cerevisiaeon the chemical composition and the sensory profile of Chardonnay wine. Coculture makes it possible to obtain completely new aromatic expressions that do not exist in the original pure cultures attributed to yeast interactions. Esters, fatty acids and phenol families were identified as affected. The sensory profiles and metabolome of the cocultures, corresponding pure cultures and associated wine blends from both pure cultures were found to be different. The coculture did not turn out to be the addition of the two pure culture wines, indicating the impact of interaction. High resolution mass spectrometry revealed thousands of cocultures biomarkers. The metabolic pathways involved in these wine composition changes were highlighted, most of them belonging to nitrogen metabolism.