bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of Stabilization Heat Treatment Inducing γ′-γ″ Co-Precipitates and η Phase on Tensile Behaviors of Inconel 706
Authors: Liu Chenglu , Gao Lei , Wu Hao , Miao Kesong , Wu He , Li Rengeng , Li Xuewen
Published: July 2024

Inconel 706 (IN706) alloy is commonly used in aircraft engines and power plant components that must meet very high performance requirements. The stabilization treatment has a significant effect on the precipitation and evolution of the reinforcing phases of the alloy, favoring the creep properties and adversely affecting the room-temperature tensile properties. However, the mechanism of the effect of the stabilization treatment on the mechanical properties of the alloys remains unclear. In this study, the effect of stabilization treatment time on the microstructure and tensile properties of IN706 alloy was investigated. The results showed that as the stabilization time gradually increased, the tensile strength remained basically unchanged (about 1250 MPa), the yield strength decreased from 1031 MPa to 985 MPa, and the plasticity decreased from 28.2% to 20.2%. The stabilization treatment induces the precipitation of granular, rod-shaped, and needle-like η phases at grain boundaries, accompanied by the appearance of a precipitate free zone (PFZ). Since the η phase is enriched with Ti and Nb, its precipitation along the grain boundary results in the depletion of Ti and Nb in the surrounding regions, thereby constraining the precipitation of the γ′ and γ″ phases, resulting in the appearance of PFZ. With increasing stabilization time, the size increase and volume fraction decrease in γ′-γ″ co-precipitates due to the precipitation of η-phase precipitates, leading to a decrease in their yield strength. Combined with in situ tensile tests, it was found that the decrease in the elongation of the stabilization treatment samples was due to the presence of η phase at the grain boundaries, which induced stress concentration and cracking at the grain boundaries. The results show that the mechanical properties of the material were gradually enhanced as the stabilization time decreased. This means it can help to choose the suitable process for IN706 alloy in different service conditions.
Optical and Morphological Characterization of Nanoscale Oxides Grown in Low-Energy H+-Implanted c-Silicon
Authors: Szekeres Anna , Alexandrova Sashka , Anastasescu Mihai , Stroescu Hermine , Gartner Mariuca , Petrik Peter
Published: July 2024

Nanoscale oxides grown in c-silicon, implanted with low-energy (2 keV) H+ ions and fluences ranging from 1013 cm−2 to 1015 cm−2 by RF plasma immersion implantation (PII), have been investigated. The oxidation of the implanted Si layers proceeded in dry O2 at temperatures of 700 °C, 750 °C and 800 °C. The optical characterization of the formed Si/SiOx structures was conducted by electroreflectance (ER) and spectroscopic ellipsometric (SE) measurements. From the ER and SE spectra analysis, the characteristic energy bands of direct electron transitions in Si are elaborated. The stress in dependence on hydrogenation conditions is considered and related to the energy shifts of the Si interband transitions around 3.4 eV. Silicon oxides, grown on PII Si at a low H+ fluence, have a non-stoichiometric nature, as revealed by IR-SE spectra analysis, while with an increasing H+ fluence in the PII Si substrates and/or the subsequent oxidation temperature the stoichiometric Si-O4 units in the oxides become predominant. The development of surface morphology is studied by atomic force microscopy (AFM) imaging. Oxidation of the H+-implanted Si surface region flattens out the surface pits created on the Si surface by H+ implants. Based on the evaluation of the texture index and mean fractal dimension, the isotropic and self-similar character of the studied surfaces is emphasized.
Evaluating the Safety of Bacillus cereus GW-01 Obtained from Sheep Rumen Chyme
Authors: Xu Bowen , Huang Xinyi , Qin Haixiong , Lei Ying , Zhao Sijia , Liu Shan , Liu Gang , Zhao Jiayuan
Published: July 2024

Bacillus cereus is responsible for 1.4–12% food poisoning outbreaks worldwide. The safety concerns associated with the applications of B. cereus in health and medicine have been controversial due to its dual role as a pathogen for foodborne diseases and a probiotic in humans and animals. In this study, the pathogenicity of B. cereus GW-01 was assessed by comparative genomic, and transcriptome analysis. Phylogenetic analysis based on a single-copy gene showed clustering of the strain GW-01, and 54 B. cereus strains from the NCBI were classified into six major groups (I–VI), which were then associated with the source region and sequence types (STs). Transcriptome results indicated that the expression of most genes related with toxins secretion in GW-01 was downregulated compared to that in the lag phase. Overall, these findings suggest that GW-01 is not directly associated with pathogenic Bacillus cereus and highlight an insightful strategy for assessing the safety of novel B. cereus strains.
Characterization of Low-Molecular-Weight Dissolved Organic Matter Using Optional Dialysis and Orbitrap Mass Spectrometry
Authors: Li Qiuxing , Zhang Runyu , Huang Guopei , Yuan Haijun , Wang Liying , Xu Shuxia
Published: July 2024

Low-molecular-weight (LMW, <1000 Da) dissolved organic matter (DOM) plays a significant role in metal/organic pollutant complexation, as well as photochemical/microbiological processes in freshwater ecosystems. The micro size and high reactivity of LMW-DOM hinder its precise characterization. In this study, Suwannee River fulvic acid (SRFA), a commonly used reference material for aquatic DOM, was applied to examine the optical features and molecular composition of LMW-DOM by combining membrane separation, ultraviolet–visible absorption and Orbitrap mass spectrometry (MS) characterization. The 100–500 Da molecular weight cut-off (MWCO) membrane had a better performance in regard to separating the tested LMW-DOM relative to the 500–1000 Da MWCO membrane. The ultraviolet–visible absorbance decreased dramatically for the retentates, whereas it increased for the dialysates. Specifically, carbohydrates, lipids and peptides exhibited high selectivity to the 100–500 Da MWCO membrane in early dialysis. Lignins, tannins and condensed aromatic molecules displayed high permeability to the 500–1000 Da MWCO membrane in late dialysis. Overall, the retentates were dominated by aromatic rings and phenolic hydroxyls with high O/Cwa (weighted average of O/C) and low H/Cwa. Conversely, such dialysates had numerous aliphatic chains with high H/Cwa and low O/Cwa compared to SRFA. In particular, LMW-DOM below 200 Da was identified by Orbitrap MS. This work provides an operational program for identifying LMW-DOM based on the SRFA standard and MS analysis.
Impact of Transglutaminase-Mediated Crosslinking on the Conformational Changes in a Dual-Protein System and IgE Reactivity of Soy Protein
Authors: Xing Guangliang , Hui Tianran , Liu Jia , Yang Siran
Published: July 2024

Transglutaminase (TGase)-catalyzed crosslinking has gained substantial traction as a novel strategy for reducing allergenic risk in food proteins, particularly within the realm of hypoallergenic food production. This study explored the impact of TGase crosslinking on conformational changes in a binary protein system composed of soy protein isolate (SPI) and sodium caseinate (SC) at varying mass ratios (10:0, 7:3, 5:5, 3:7 (w/w)). Specifically, the immunoglobulin E (IgE) binding capacity of soy proteins within this system was examined. Prolonged TGase crosslinking (ranging from 0 h to 15 h) resulted in a gradual reduction in IgE reactivity across all SPI-SC ratios, with the order of IgE-binding capability as follows: SPI > SPI5-SC5 > SPI7-SC3 > SPI3-SC7. These alterations in protein conformation following TGase crosslinking, as demonstrated by variable intrinsic fluorescence, altered surface hydrophobicity, increased ultraviolet absorption and reduced free sulfhydryl content, were identified as the underlying causes. Additionally, ionic bonds were found to play a significant role in maintaining the structure of the dual-protein system after crosslinking, with hydrophobic forces and hydrogen bonds serving as supplementary forces. Generally, the dual-protein system may exhibit enhanced efficacy in reducing the allergenicity of soy protein.
Preparation and Application of Co-Doped Zinc Oxide: A Review
Authors: Luo Zhaoyu , Rong Ping , Yang Zhiyuan , Zhang Jianhua , Zou Xiangyu , Yu Qi
Published: July 2024

Due to a wide band gap and large exciton binding energy, zinc oxide (ZnO) is currently receiving much attention in various areas, and can be prepared in various forms including nanorods, nanowires, nanoflowers, and so on. The reliability of ZnO produced by a single dopant is unstable, which in turn promotes the development of co-doping techniques. Co-doping is a very promising technique to effectively modulate the optical, electrical, magnetic, and photocatalytic properties of ZnO, as well as the ability to form various structures. In this paper, the important advances in co-doped ZnO nanomaterials are summarized, as well as the preparation of co-doped ZnO nanomaterials by using different methods, including hydrothermal, solvothermal, sol-gel, and acoustic chemistry. In addition, the wide range of applications of co-doped ZnO nanomaterials in photocatalysis, solar cells, gas sensors, and biomedicine are discussed. Finally, the challenges and future prospects in the field of co-doped ZnO nanomaterials are also elucidated.
Exploration of Free Energy Surface of the Au10 Nanocluster at Finite Temperature
Authors: Rojas-González Francisco Eduardo , Castillo-Quevedo César , Rodríguez-Kessler Peter Ludwig , Jimenez-Halla José Oscar Carlos , Vásquez-Espinal Alejandro , Eithiraj Rajagopal Dashinamoorthy , Cortez-Valadez Manuel , Cabellos José Luis
Published: July 2024

The first step in comprehending the properties of Au10 clusters is understanding the lowest energy structure at low and high temperatures. Functional materials operate at finite temperatures; however, energy computations employing density functional theory (DFT) methodology are typically carried out at zero temperature, leaving many properties unexplored. This study explored the potential and free energy surface of the neutral Au10 nanocluster at a finite temperature, employing a genetic algorithm coupled with DFT and nanothermodynamics. Furthermore, we computed the thermal population and infrared Boltzmann spectrum at a finite temperature and compared it with the validated experimental data. Moreover, we performed the chemical bonding analysis using the quantum theory of atoms in molecules (QTAIM) approach and the adaptive natural density partitioning method (AdNDP) to shed light on the bonding of Au atoms in the low-energy structures. In the calculations, we take into consideration the relativistic effects through the zero-order regular approximation (ZORA), the dispersion through Grimme’s dispersion with Becke–Johnson damping (D3BJ), and we employed nanothermodynamics to consider temperature contributions. Small Au clusters prefer the planar shape, and the transition from 2D to 3D could take place at atomic clusters consisting of ten atoms, which could be affected by temperature, relativistic effects, and dispersion. We analyzed the energetic ordering of structures calculated using DFT with ZORA and single-point energy calculation employing the DLPNO-CCSD(T) methodology. Our findings indicate that the planar lowest energy structure computed with DFT is not the lowest energy structure computed at the DLPN0-CCSD(T) level of theory. The computed thermal population indicates that the 2D elongated hexagon configuration strongly dominates at a temperature range of 50–800 K. Based on the thermal population, at a temperature of 100 K, the computed IR Boltzmann spectrum agrees with the experimental IR spectrum. The chemical bonding analysis on the lowest energy structure indicates that the cluster bond is due only to the electrons of the 6 s orbital, and the Au d orbitals do not participate in the bonding of this system.
Applications of Nanozymes in Chiral-Molecule Recognition through Electrochemical and Ultraviolet–Visible Analysis
Authors: Dai Jing-Jing , Chen Guo-Ying , Xu Lei , Zhu Huan , Yang Feng-Qing
Published: July 2024

Chiral molecules have similar physicochemical properties, which are different in terms of physiological activities and toxicities, rendering their differentiation and recognition highly significant. Nanozymes, which are nanomaterials with inherent enzyme-like activities, have garnered significant interest owing to their high cost-effectiveness, enhanced stability, and straightforward synthesis. However, constructing nanozymes with high activity and enantioselectivity remains a significant challenge. This review briefly introduces the synthesis methods of chiral nanozymes and systematically summarizes the latest research progress in enantioselective recognition of chiral molecules based on electrochemical methods and ultraviolet–visible absorption spectroscopy. Moreover, the challenges and development trends in developing enantioselective nanozymes are discussed. It is expected that this review will provide new ideas for the design of multifunctional chiral nanozymes and broaden the application field of nanozymes.
Optimization of Enzyme-Assisted Extraction of Rosemary Essential Oil Using Response Surface Methodology and Its Antioxidant Activity by Activating Nrf2 Signaling Pathway
Authors: Li Yuanyuan , Huang Lei , Xu Yongfang , Cheng Biao , Zhao Mingqin
Published: July 2024

Rosemary essential oil (REO) is widely recognized as a food flavoring and traditional herb and possesses potential antioxidant activity. However, its low yield rate and unclarified antioxidant mechanism warrant further investigation. In this study, an enzyme pretreatment-assisted extraction method with Box–Behnken design (BBD) and response surface methodology (RSM) models was employed to optimize the main factors of REO, and its antioxidant molecular mechanism under oxidative stress was elucidated in hydrogen peroxide-induced human lung carcinoma (A549) cells. The optimized yield (4.10%) of REO was recorded with the following optimum conditions: enzyme amount 1.60%, enzyme digestion pH 5.0, enzyme digestion temperature 46.50 °C, and enzyme digestion time 1.7 h. Meanwhile, 1.8-cineole (53.48%) and β-pinene (20.23%) exhibited radical scavenging activity higher than that of BHA and BHT. At the cellular level, REO (12.5–50 µg/mL) increased the levels of cell viability, CAT, SOD, and GSH significantly while reducing the contents of ROS, MDA, and GSSG, when compared to H2O2 exposure. Mechanically, REO relieved oxidative stress via activating the Nrf2 signaling pathway and enhancing the protein expression of Nrf2, NQO-1, and HO-1, which was further verified by molecular docking between the main component 1.8-cineole and the Kelch domain of KEAP1. Therefore, REO could be considered as a potent natural antioxidant with a potential strategy in the food and pharmaceutical industries.
Evaluation of Anticancer Activity of Nucleoside–Nitric Oxide Photo-Donor Hybrids
Authors: Marchesi Elena , Melloni Elisabetta , Casciano Fabio , Pozza Elena , Argazzi Roberto , De Risi Carmela , Preti Lorenzo , Perrone Daniela , Navacchia Maria Luisa
Published: July 2024

Herein, we report the synthesis of a new hybrid compound based on a 2′-deoxyuridine nucleoside conjugated with a NO photo-donor moiety (dU-t-NO) via CuAAC click chemistry. Hybrid dU-t-NO, as well as two previously reported 2′-deoxyadenosine based hybrids (dAdo-S-NO and dAdo-t-NO), were evaluated for their cytotoxic and cytostatic activities in selected cancer cell lines. dAdo-S-NO and dAdo-t-NO hybrids displayed higher activity with respect to dU-t-NO. All hybrids showed effective release of NO in the micromolar range. The photochemical behavior of the newly reported hybrid, dU-t-NO, was studied in the RKO colon carcinoma cell line, whereas the dAdo-t-NO hybrid was tested in both colon carcinoma RKO and hepatocarcinoma Hep 3B2.1-7 cell lines to evaluate the potential effect of NO released upon irradiation on cell viability. A customized irradiation apparatus for in vitro experiments was also designed.
Repellency, Toxicity, and Chemical Composition of Plant Essential Oils from Myrtaceae against Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera Liviidae)
Authors: Li Yi-Jie , Liu Tian-Ao , Zhao Hang , Han Yang , Lou Bing-Hai , Lei Cui-Yun , Song Ya-Qin , Jiang Hong-Bo
Published: July 2024

Diaphorina citri Kuwayama (D. citri) is one of the major pests in the citrus industry, which spreads Citrus Huanglongbing disease. It has developed resistance to chemical insecticides. Therefore, searching for greener solutions for pest management is critically important. The main aim of this study was to evaluate the repellent and insecticidal efficacy of essential oils (EOs) from four species of Myrtaceae plants: Psidium guajava (PG), Eucalyptus robusta (ER), Eucalyptus tereticornis (ET), and Baeckea frutescens (BF) against D. citri and to analyze their chemical compositions. GC-MS analysis was performed, and the results indicated that the EOs of PG, ER, ET, and BF were rich in terpenoids, ketones, esters, and alcohol compounds. The repellent rate of all four EOs showed that it decreased with exposure time but increased with the concentration of EOs from 80.50% to 100.00% after treating D. citri for 6 h with four EOs at 100% concentration and decreased to 67.71% to 85.49% after 24 h of exposure. Among the compounds from the EOs tested, eucalyptol had the strongest repellent activity, with a 24 h repellency rate of 100%. The contact toxicity bioassay results showed that all EOs have insecticidal toxicity to D. citri; the LC50 for nymphs was 36.47–93.15 mL/L, and for adults, it was 60.72–111.00 mL/L. These results show that when PG is used as the reference material, the ER, ET, and BF EOs have strong biological activity against D. citri, which provides a scientific basis for the further development of plant-derived agrochemicals.
Growth of α-Ga2O3 from Gallium Acetylacetonate under HCl Support by Mist Chemical Vapor Deposition
Authors: Yasuoka Tatsuya , Liu Li , Dang Giang T. , Kawaharamura Toshiyuki
Published: July 2024

α-Ga2O3 films were grown on a c-plane sapphire substrate by HCl-supported mist chemical vapor deposition with multiple solution chambers, and the effect of HCl support on α-Ga2O3 film quality was investigated. The growth rate monotonically increased with increasing Ga supply rate. However, as the Ga supply rate was higher than 0.1 mmol/min, the growth rate further increased with increasing HCl supply rate. The surface roughness was improved by HCl support when the Ga supply rate was smaller than 0.07 mmol/min. The crystallinity of the α-Ga2O3 films exhibited an improvement with an increase in the film thickness, regardless of the solution preparation conditions, Ga supply rate, and HCl supply rate. These results indicate that there is a low correlation between the improvement of surface roughness and crystallinity in the α-Ga2O3 films grown under the conditions described in this paper.
The Contribution of the Triage Nurse in the Optimisation of Door-to-Computed-Tomography Time in Stroke
Authors: Antunes Raquel , Costeira Cristina , Pereira Sousa Joana , Santos Cátia
Published: July 2024

A stroke is a time-sensitive emergency, so diagnosing and treating the victim promptly is extremely important. Therefore, the purpose of this study was to identify the influence of the Stroke Code Protocol’s activation on the door-to-computed-tomography (door-to-CT) time and determine whether factors such as previous Modified Rankin Scale (mRS), age, and gender influence its activation. A retrospective study was conducted in a Medical-Surgical Emergency Department in the centre of Portugal from 1 January 2021 to 31 December 2022. The sample was selected according to the diagnosis assigned at the time of clinical discharge from the Emergency Department and the Stroke Code Protocol activation criteria. It was observed that 113 (50%) suspected stroke victims who met the activation criteria for the Stroke Code Protocol did not have the protocol activated, which had a highly significant influence (p < 0.001) on door-to-CT time. It was determined that activation at triage has an average door-to-CT time of 35 ± 18 min, post-triage activation has an average door-to-CT time of 38 ± 26 min, and non-activation has an average door-to-CT time of 1 h 04 ± 45 min. The need to implement an institutional protocol for activating the Stroke Code Protocol and provide specialised training for the multidisciplinary team is reiterated.
Muscular Oxygen Saturation and Hemoglobin Concentration during Freediving: A Case Study
Authors: Vrdoljak Dario , Dujić Željko , Foretić Nikola
Published: July 2024

Lower limb muscle fatigue is the main reason for withdrawal from diving. Therefore, this study aimed to determine the local muscle oxygen saturation and hemoglobin concentration in the vastus lateralis muscle during different freediving disciplines. One freediver participated in this study, and his chronological age was 40 years, body mass 75.0 kg, body height 184.0 cm, and body fat 13.7%. The participant has been practicing freediving for 6 years. The variables in this study included anthropometric indices, heart rate, and muscle oxygen dynamics parameters (SmO2 (oxygen muscle saturation) and tHb (total hemoglobin)). The variables were measured during five diving disciplines: static apnea, bifin, dynamic no fins (DNF), monofin, and sneaking. Measurements were performed during intensive training/competition during the diving season in August 2023. The results of this study showed that when oxygen starts to decrease during the dive, the tHb increases. Furthermore, the times at which maximal tHb and minimal SmO2 were achieved are also shown. These parameters occurred at almost the same time across all disciplines: static (SmO2, 142; tHb, 150 s), bifin (SmO2, 153; tHb, 148 s), DNF (SmO2, 162; tHb, 178 s), monofin (SmO2, 96; tHb, 94 s), and sneaking (SmO2, 212; tHb, 228 s). Also, differences in tHb and SmO2 were present between diving disciplines. In particular, the highest increase in tHb was present in bifin (0.0028 AU/s), whereas monofin showed a decrease (−0.0009 AU/s). On the other hand, the highest desaturation was seen in bifin (−0.87%/s) and the lowest in sneaking (−0.29%/s) These findings emphasize the physiological characteristics of freedivers engaging in different freediving disciplines that influence muscles during the dive. Such responses could be observed through a concurrent hypoxia/hypercapnia and a transient reduction in the Fahraeus effect.
VCSELs with Stable Linear Polarization Emission Induced by Dielectric Columnar Thin Film Mirrors
Authors: Panajotov Krassimir
Published: July 2024

We propose and analyze numerically new approaches to force the laser emission from VCSELs in a well-defined linear polarization independent of the existing phase and amplitude anisotropies by using dielectric columnar thin-film (CTF) layers in the distributed Bragg reflector (DBR). In one approach, we have demonstrated CTF-VCSELs with top DBR consisting of two alternating CTF layers grown in orthogonally oriented planes and with high and low refractive index for one linear polarization while having the same value of the refractive index value for the orthogonal linear polarization. Such CTF-VCSELs have large dichroism of the mirror losses for two orthogonal linear polarizations. We have also shown DBR designs with parallel columnar orientations of the two CTF dielectric materials. In a second approach, we implement only one CTF layer in the dielectric DBR chosen in such a way that only one linearly polarized longitudinal mode is resonant in the CTF-VCSEL while light with the orthogonally oriented linear polarization is out of resonance and thus cannot lase. Simple estimation of the polarization mode suppression ratio for the different exemplary designs of CTF-VCSELs based on TiO2 and TaO2 dielectric CTFs results in values as high as 80 dB, which compares favorably to the existing alternative approaches.
Discovering Bohr’s Yin-Yang Diagram in Quantum Tunneling Dynamics
Authors: Yang Ciann-Dong
Published: July 2024

On 17 October 1947, Niels Bohr was made a knight of the Order of the Elephant by the King of Denmark in view of his outstanding achievements and contributions to science. Bohr designed his own coat of arms that featured a pattern of Yin and Yang (Tai Chi symbol) to symbolize the wave–particle complementarity. However, Bohr’s Yin-Yang diagram (YYD) was neither drawn based on the principles of quantum mechanics, nor did it originate from the traditional Taoist YYD. Scientists still have doubts about the legitimacy of using YYD as the icon of the wave–particle complementarity, because the YYD belonging to quantum mechanics itself is unknown so far. This paper reports the YYDs existing in quantum mechanics and justifies the role of YYD in the wave–particle duality by showing that any system, whether classical or quantum, has an ideal YYD as long as it satisfies Bohr’s principle of complementarity (BPC). The deviation of a deformed YYD from the ideal YYD indicates the extent to which a real system satisfies BPC. This paper constructs the quantum YYD by the complex quantum trajectory of a particle tunneling via a step barrier, which displays the continuous transition between the wave behavior and the particle behavior. It appears that the YYD designed by Bohr in his coat of arms resembles the YYD generated by tunneling motion, not only in appearance but also in the governing equation.
Two Carya Species, Carya hunanensis and Carya illinoinensis, Used as Rootstocks Point to Improvements in the Heat Resistance of Carya cathayensis
Authors: Huang Shanxia , Xu Yanxia , Li Xueqin , Ye Boyu , Jin Songheng
Published: July 2024

Grafting as a crucial horticultural technique has been widely used in the cultivation of Carya cathayensis (Chinese hickory), which is a unique and important economic tree in the northeast of Zhejiang Province and the south of Anhui Province. However, the existing literature lacks research on the potential impact of various rootstocks on the thermal tolerance of Chinese hickory. The objectives of this study were to evaluate heat tolerance in four distinct groups of Chinese hickory, including C. cathayensis grafted onto Carya hunanensis and Carya illinoinensis, one self-grafted group (C. cathayensis grafted onto C. cathayensis), and one non-grafted group (C. cathayensis). We examined photosynthesis parameters, phytohormones, and differentially expressed genes in the four various hickory groups subjected to 25 °C, 35 °C, and 40 °C heat stress (HS). The results demonstrated that grafting onto C. hunanensis and C. illinoinensis exhibited a higher net photosynthetic rate and stomatal conductance, lower intercellular CO2 concentration, and smaller changes in plant hormone content compared to self-grafted and non-grafted group under HS. The transcriptome results revealed that the majority of differentially expressed genes (DEGs) associated with photosynthetic pathways exhibited downregulation under HS, while the degree of variation in grafted groups using C. hunanensis and C. illinoinensis as rootstocks was comparatively lower than that observed in self-grafted and non-grafted groups. The alteration in the expression patterns of DEGs involved in plant hormone synthesis and metabolism under HS corresponded to changes in plant hormone contents. Overall, Chinese hickory grafted onto C. hunanensis and C. illinoinensis exhibited enhanced resistance to high-temperature stress at the juvenile stage.
Exploring the Potential of Silicon Tetrachloride as an Additive in CO2-Based Binary Mixtures in Transcritical Organic Rankine Cycle—A Comparative Study with Traditional Hydrocarbons
Authors: Alazwari Mashhour A. , Siddiqui Muhammad Ehtisham
Published: July 2024

Carbon dioxide (CO2) has been recognized as one of the potential working fluids to operate power generation cycles, either in supercritical or transcritical configuration. However, a small concentration of some of the additives to CO2 have shown promising improvements in the overall performance of the cycle. The current study is motivated by the newly proposed additive silicon tetrachloride (SiCl4), and so we perform a detailed investigation of SiCl4 along with a few well-known additives to CO2-based binary mixtures as a working fluid in transcritical organic Rankine cycle setup with internal heat regeneration. The additives selected for the study are pentane, cyclopentane, cyclohexane, and silicon tetrachloride (SiCl4). A comprehensive study on the energy and exergy performance of the cycle for warm regions is conducted at a turbine inlet temperature of 250 °C. The performance of the heat recovery unit is also assessed to highlight its importance in comparison to a simple configuration of the cycle. This study shows that the cycle operating with binary mixtures performs significantly better than with pure CO2, which is mainly due to its better heat recovery in the heat recovery unit. The results show that the optimal molar concentration of the additives is in between 20% and 25%. Besides having better thermal stability, SiCl4 shows an improvement in the cycle thermal efficiency by 6% points which is comparable to cyclopentane (7.3% points) and cyclohexane (7.8% points). The optimal cycle pressure ratio for SiCl4 is also relatively lower than for other additives. The energy efficiency of the cycle with pure CO2 is around 45% which is also increased to 58%, 63%, 64%, 60% with pentane, cyclopentane, cyclohexane, and SiCl4, respectively. These results suggest that additives like SiCl4 could make CO2-based cycles more viable for power generation in warm regions.
Continuous Online Semantic Implicit Representation for Autonomous Ground Robot Navigation in Unstructured Environments
Authors: Serdel Quentin , Marzat Julien , Moras Julien
Published: July 2024

While mobile ground robots have now the physical capacity of travelling in unstructured challenging environments such as extraterrestrial surfaces or devastated terrains, their safe and efficient autonomous navigation has yet to be improved before entrusting them with complex unsupervised missions in such conditions. Recent advances in machine learning applied to semantic scene understanding and environment representations, coupled with modern embedded computational means and sensors hold promising potential in this matter. This paper therefore introduces the combination of semantic understanding, continuous implicit environment representation and smooth informed path-planning in a new method named COSMAu-Nav. It is specifically dedicated to autonomous ground robot navigation in unstructured environments and adaptable for embedded, real-time usage without requiring any form of telecommunication. Data clustering and Gaussian processes are employed to perform online regression of the environment topography, occupancy and terrain traversability from 3D semantic point clouds while providing an uncertainty modeling. The continuous and differentiable properties of Gaussian processes allow gradient based optimisation to be used for smooth local path-planning with respect to the terrain properties. The proposed pipeline has been evaluated and compared with two reference 3D semantic mapping methods in terms of quality of representation under localisation and semantic segmentation uncertainty using a Gazebo simulation, derived from the 3DRMS dataset. Its computational requirements have been evaluated using the Rellis-3D real world dataset. It has been implemented on a real ground robot and successfully employed for its autonomous navigation in a previously unknown outdoor environment.
Effect of Culture Temperatures on the Initial Growth Performance of Seedlings Germinated from Cryostored Seeds of a Tropical Tree Parkia nitida Miq. (Fabaceae, Mimosoideae)
Authors: Maruyama Tsuyoshi E. , Tsuruta Momi , Mori Tokunori
Published: July 2024

Seedling growth is one of the most important stages for the establishment of natural and artificial regeneration. For the first time, the initial growth and biomass allocation of seedlings germinated from cryostored seeds of Parkia nitida were analyzed. P. nitida is an economically and ecologically important timber tree species distributed in Central and South America. Cryostored seeds germinated quickly after priming by scarifying a part of the seedcoat with emery paper, reaching a germination percentage of 94%. Thirteen weeks after germination, the seedlings grew to a height of 16.5 to 60.0 cm. The results of our study, under different day/night alternating culture temperatures, showed that culture temperature had a direct correlation with seedling growth, total biomass allocation, and biomass partitioning. The greatest growth (height, diameter, and number of node sections) and greatest biomass allocation (leaf, stem, and root weight) were recorded under alternating temperatures of 30/25 °C, and these decreased with decreasing culture temperatures to 25/20 °C and 20/15 °C. Shoot:Root (S:R) ratios also decreased with decreasing culture temperatures, but a statistically significant difference (p < 0.05) was only observed between 20/15 °C and 30/25 °C. However, significant differences were not observed in Photosynthetic:Non-photosynthetic organ ratios among the different alternating culture temperatures. This study provides fundamental information for the production of good-quality seedlings of the fast-growing tropical trees of the legume family.
AI-Driven Prediction and Mapping of Soil Liquefaction Risks for Enhancing Earthquake Resilience in Smart Cities
Authors: Katsuumi Arisa , Cong Yuxin , Inazumi Shinya
Published: July 2024

In response to increasing urbanization and the need for infrastructure resilient to natural hazards, this study introduces an AI-driven predictive model designed to assess the risk of soil liquefaction. Utilizing advanced ensemble machine learning techniques, the model integrates geotechnical and geographical data to accurately predict the potential for soil liquefaction in urban areas, with a specific focus on Yokohama, Japan. This methodology leverages comprehensive datasets from geological surveys and seismic activity to enhance urban planning and infrastructure development in smart cities. The primary outputs include detailed soil liquefaction risk maps that are essential for effective urban risk management. These maps support urban planners and engineers in making informed decisions, prioritizing safety, and promoting sustainability. The model employs a robust combination of artificial neural networks and gradient boosting decision trees to analyze and predict data points, assessing soil susceptibility to liquefaction during seismic events. Notably, the model achieves high accuracy in predicting soil classifications and N-values, which are critical for evaluating soil liquefaction risk. Validation against an extensive dataset from geotechnical surveys confirms the model’s practical effectiveness. Moreover, the results highlight the transformative potential of AI in enhancing geotechnical risk assessments and improving the resilience of urban areas against natural hazards.
Mapping Petri Nets onto a Calculus of Context-Aware Ambients
Authors: Siewe François , Germanos Vasileios , Zeng Wen
Published: July 2024

Petri nets are a graphical notation for describing a class of discrete event dynamic systems whose behaviours are characterised by concurrency, synchronisation, mutual exclusion and conflict. They have been used over the years for the modelling of various distributed systems applications. With the advent of pervasive systems and the Internet of Things, the Calculus of Context-aware Ambients (CCA) has emerged as a suitable formal notation for analysing the behaviours of these systems. In this paper, we are interested in comparing the expressive power of Petri nets to that of CCA. That is, can the class of systems represented by Petri nets be modelled in CCA? To answer this question, an algorithm is proposed that maps any Petri net onto a CCA process. We prove that a Petri net and its corresponding CCA process are behavioural equivalent. It follows that CCA is at least as expressive as Petri nets, i.e., any system that can be specified in Petri nets can also be specified in CCA. Moreover, tools developed for CCA can also be used to analyse the behaviours of Petri nets.
Limits of Harmonic Stability Analysis for Commercially Available Single-Phase Inverters for Photovoltaic Applications
Authors: Kaufhold Elias , Meyer Jan , Myrzik Johanna , Schegner Peter
Published: July 2024

The growth of renewables in public energy networks requires suitable strategies to assess the stable operation of the respective power electronic devices, e.g., inverters. Different assessment methods can be performed with regard to the available knowledge and the assessment objective, e.g., a specific frequency range or the input signal characteristics that are typically classified into small-signal and large-signal disturbances. This paper addresses the limits of the measurement-based small-signal stability analysis in the harmonic frequency range of commercially available single-phase inverters for photovoltaic applications. The harmonic stability is analyzed, and the results for a sinusoidal background voltage and distorted background voltages are assessed based on measurements. The measurements prove that even in the harmonic frequency range, the harmonic stability analysis can only provide a sufficient but not a necessary condition in terms of the statement towards an instable operation.
Nitrate and Nitrite Exposure Induces Visual Impairments in Adult Zebrafish
Authors: Saputra Febriyansyah , Kishida Mitsuyo , Hu Shao-Yang
Published: July 2024

Nitrate and nitrite have emerged as increasingly common environmental pollutants, posing significant risks to various forms of life within ecosystems. To understand their impact on the visual system of zebrafish, adult zebrafish were exposed to environmentally relevant concentrations of nitrate (10 mg/L) and nitrite (1 mg/L) for 7 days. Visual behaviors were examined using optomotor and avoidance response. The eyeballs of the zebrafish were collected for H&E staining, IHC, and qPCR. Exposure decreased visual behavior and the thickness of most retinal layers. Exposure decreased expression of pax6a, pax6b, gpx1a, and bcl2a. Exposure increased expression of esr1, esr1a, esr2b, cyp19a1b, sod1a, nos2a, casps3, and tp53, and increased retinal brain aromatase expression by IHC. Collectively, our findings demonstrate that nitrate and nitrite exposure negatively impacted the visual system of adult zebrafish, highlighting the potential hazards of these environmental pollutants on aquatic organisms.
Rabies Realities: Navigating Barriers to Rabies Control in Rural Zambia—A Case Study of Manyinga and Mwansabombwe Districts
Authors: Misapa Muma Chipo , Bwalya Eugene C. , Moonga Ladslav , Zimba Josiah , Kabwali Emmanuel S. , Silombe Mwenya , Mulwanda Edgar Chilanzi , Mulenga Christopher , Simuunza Martin C. , Sawa Hirofumi , Hang’ombe Bernard , Muleya Walter
Published: July 2024

Rabies persists as a longstanding issue in Zambia, despite being preventable. The current control measures, including dog vaccination, population control, and movement restriction, guided by ‘The Control of Dogs Act Chapter 247 of the Laws of Zambia’, have not yielded the desired impact in many areas of the country including Manyinga and Mwansabombwe districts. These two districts continue to report low dog vaccination rates, unrestricted dog movements, and escalating cases of animal and human rabies, along with dog bites. Aligned with global aspirations to achieve zero human rabies cases by 2030, this study scrutinizes the determinants and obstacles hampering the execution of rabies control initiatives in Manyinga and Mwansabombwe. Spanning approximately 11 months, this cross-sectional study gathered pre- and post-vaccination data from 301 households in Manyinga and 100 households in Mwansabombwe. Questionnaires probed knowledge, attitudes, and practices related to rabies prevention and control. A transect survey, key informant interviews, and assessment of rabies vaccination and dog bite records complemented the data collection. Findings revealed that 88.0% of respondents from both districts possessed knowledge about rabies, confirming affected species and transmission. Moreover, 76.8% in Manyinga and 88.6% in Mwansabombwe were acquainted with rabies prevention and control methods. Concerning dog owners, 89.0% were aware of rabies, 66.0% understood its prevention and control, and the majority identified bites as the primary mode of transmission. Despite the high level of knowledge recorded during the survey, the implementation of preventive measures was low, which was attributed to low levels of law enforcement by the local government authority, inadequate staffing in the veterinary department, unwillingness to pay for dog vaccinations, and unavailability of rabies vaccine at the veterinary office in both districts. Vaccination coverage stood at 64.0% in Manyinga and 21.0% in Mwansabombwe. Notably, education and occupation exhibited a positive significant association with rabies knowledge. In terms of dog bite cases, Manyinga recorded 538 dog bite cases from 2017 to June 2022, while Mwansabombwe recorded 81 dog bite and 23 jackal bite cases from 2021 to June 2022. The study underscores critical knowledge gaps in rural areas and emphasizes the imperative for enhanced public education and awareness programs, improved rabies surveillance, free mass vaccination campaigns, and community engagement to augment vaccination coverage and knowledge about rabies.
Macropinocytosis Is the Principal Uptake Mechanism of Antigen-Presenting Cells for Allergen-Specific Virus-like Nanoparticles
Authors: Kraus Armin , Kratzer Bernhard , Sehgal Al Nasar Ahmed , Trapin Doris , Khan Matarr , Boucheron Nicole , Pickl Winfried F.
Published: July 2024

Virus-like nanoparticles (VNP) are regarded as efficient vaccination platforms and have proven to be useful for the non-anaphylactogenic delivery of allergen-specific immunotherapy in preclinical models previously. Herein, we sought to determine the mode of VNP uptake by antigen presenting cells (APC). Accordingly, we screened a collection of substances known to inhibit different uptake pathways by APC. The human leukemia monocytic cell line THP-1 and the murine dendritic cell line DC 2.4 were examined for the uptake of fluorescently labelled VNP in the presence or absence of inhibitors. The inhibitory effect of candidate substances that blocked VNP uptake in APC lines was subsequently evaluated in studies with primary APC present in splenocyte and lung cell homogenates in vitro and upon intratracheal application of VNP in vivo. The uptake of allergen-specific VNP in vitro and in vivo was mainly observed by macrophages and CD103+ dendritic cells and was sensitive to inhibitors that block macropinocytosis, such as hyperosmolarity induced by sucrose or the polyphenol compound Rottlerin at low micromolar concentrations but not by other inhibitors. Also, T-cell proliferation induced by allergen-specific VNP was significantly reduced by both substances. In contrast, substances that stimulate macropinocytosis, such as Heparin and phorbol myristate acetate (PMA), increased VNP-uptake and may, thus, help modulate allergen-specific T-cell responses. We have identified macropinocytosis as the principal uptake mechanism of APC for allergen-specific VNP in vitro and in vivo, paving the way for further improvement of VNP-based therapies, especially those that can be used for tolerance induction in allergy, in the future.
Effect of Breed and Season in Buck Semen Cryopreservation: The Portuguese Animal Germplasm Bank
Authors: Barbas João Pedro , Baptista Maria Conceição , Carolino Nuno , Simões João , Margatho Gisele , Pimenta Jorge , Claudino Francisca , Ferreira Filipa Costa , Grilo Francisco , Pereira Rosa Maria Lino Neto
Published: July 2024

The aims of this study were to characterize the semen as well as the influence of breed, season, and semen processing on spermatozoa (SPZ) traits of four native Portuguese goat breeds used for the bank of Portuguese animal germplasm (BPAG). A total of 1017 ejaculates from Serrana (n = 30), Bravia (n = 15), Charnequeira (n = 11), and Preta de Montezinho (n = 3) bucks were collected between 2004 and 2020 at (EZN-INIAV; 39° N) during the whole year under natural conditions. All the fresh and cryopreserved (−196 °C) semen was evaluated and stored in the BPAG. Bravia bucks (the smallest breed) produced less (p < 0.05) volume of ejaculate than all the other breeds, which was higher during the full breeding season (September–January; p < 0.05), regarding all the other breeds. Contrarily, in general, SPZ concentration was lower during September–January, but total SPZ per ejaculate remained similar (p > 0.05) during May–August and September–January in Serrana bucks. The SPZ viability and SPZ midpiece defects were slightly influenced by breed and SPZ head defects by season (lowest % in February–April; p < 0.05). On the contrary, the freezing–thawing cycle strongly influenced (p < 0.01) all SPZ traits. The correlation coefficients of these traits between fresh and thawed SPZ were low (up to 0.33; p < 0.01), highlighting the importance of semen processing in semen cryopreservation. We conclude that breed and season had a relevant effect on ejaculate traits, but it was much less evident for the studied SPZ traits. These native goats can serve as semen donors throughout the year, under natural conditions.
Variables asociadas y desafíos en la psicoterapia sexual con parejas adultas: Un enfoque en la intimidad y satisfacción
Authors: Almagro Villegas Doménica Vanniuska , Jaramillo Oyervide Jéssica Alexandra
Published: July 2024

La psicoterapia sexual es fundamental para abordar problemas relacionados con la intimidad y la satisfacción sexual en parejas adultas. Este estudio tiene como propósito analizar las variables asociadas y los desafíos en este contexto específico. Mediante una revisión sistemática descriptiva, se exploraron publicaciones de los últimos 7 años en inglés y español, incluyendo análisis de casos y estudios descriptivos accesibles en Scopus, Web of Science y PubMed. Inicialmente, se identificaron 245 registros, de los cuales se eliminaron 68 duplicados. Tras revisar títulos y resúmenes, se seleccionaron 15 estudios elegibles, excluyendo metaanálisis. Se encontró que la intimidad sexual en parejas adultas se ve afectada por la predisposición a establecer vínculos emocionales interdependientes y una comunicación empática. Con el tiempo, la intimidad tendía a disminuir, a menudo gestionada con indiferencia para resolver conflictos. Este análisis subraya la importancia de abordar estos aspectos en la psicoterapia sexual para mejorar la calidad de vida en las relaciones de pareja.
New opportunities for individual investment in the new political reality: IT services and FinTech opportunities
Authors: Kishkovich Yu. P.
Published: July 2024

This article attempts to analytically consider the resources and capabilities of IT services and financial technologies for the needs and requirements of individual investment in a new political reality. It is indicated that digital tools can serve as an important resource for individual investment, taking into account the peculiarities of the new conditions. The problem of individual investment has been considered in numerous academic papers, but this article is an attempt at a first – of-its-kind study that actualizes this economic and financial practice in a specific analytical perspective of the new reality. According to the results of the study, the author comes to the conclusion that digital resources (IT services and Fintech), primarily on mobile devices, can serve as a significant component optimizing the effectiveness of investment projects and individual investment as a longterm economic project.