bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of a Modeling and Computational Thinking Professional Development Program on STEM Educators’ Perceptions toward Teaching Science and Engineering Practices
Authors: Colclasure Blake C. , Durham Brooks Tessa , Helikar Tomáš , King Scott J. , Webb Audrey
Published: August 2022

Teachers’ integration of the Next Generation Science Standards and corresponding Science and Engineering Practices (SEPs) illustrate current science education reform in the United States. Effective teacher professional development (PD) on SEPs is essential for reform success. In this study, we evaluated the Nebraska STEM Education Conference, a PD program for middle school, high school, and first- and second-year post-secondary STEM teachers. This SEP-oriented PD program focused predominantly on the SEPs ‘developing and using models’ and ‘using mathematics and computational thinking.’ An electronic survey was used to measure participants’ (n = 45) prior integration of SEPs, influential factors and barriers to using SEPs, and changes to interest and confidence in using SEPs as a result of attending the PD program. Our results showed that teachers had limited prior use of SEPs in their teaching. Student interest and learning outcomes were the factors found to be most influential to teachers’ use of SEPs, while limited knowledge, confidence, and resources were the most commonly identified barriers. As a result of attending the PD program, participants significantly improved their confidence and interest to incorporate SEPs. We recommend continued SEP-oriented PD to foster successful NGSS integration and to advance reforms in science education.
A Novel Label-Free Electrochemical Immunosensor Based on a Self-Assembled Monolayer-Modified Electrode for Polychlorinated Biphenyl (PCB) in Environmental Analysis
Authors: Alsefri Samia , Balbaied Thanih , Albalawi Ibtihaj , Alatawi Hanan , Moore Eric
Published: August 2022

PCBs (polychlorinated biphenyls) are a very large group of organic compounds that have between two and ten chlorine atoms attached to the biphenyl. These compounds have an acute impact as environmental pollutants, causing cancer and other adverse health effects in humans. It is therefore imperative to develop techniques for the cost-effective detection of PCBs at very low concentrations in ecosystems. In this paper, a novel label-free, indirect, competitive electrochemical immunosensor was first developed with a PCB-BSA conjugate. It is shown herein to compete with free PCBs for binding to the anti-PCB polyclonal primary antibody (IgY). Then, we used a secondary antibody to enhance the sensitivity of the sensor for the detection of PCB in a sample. It has been successfully immobilized on an 11-mercaptoundecanoic acid (11-MUA)-modified gold electrode via a carbodiimide-coupling reaction using cross-linking 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) on the electrode surface. The immunosensor was investigated by cyclic voltammetry and differential pulse voltammetry in a standard solution of [Fe(CN)6]3−/4−. A linear range of 0.011–220 ng/mL−1 and a limit of detection (LOD) of 0.11 ng/mL−1 for PCBs detection were achieved by the developed immunosensor, showing advantages over conventional assays. The novel label-free electrochemical immunosensor discussed in this paper is a solution for simple, rapid, cost-effective sample screening in a portable, disposable format. The proposed immunosensor has good sensitivity, and it can prove to be an adequate real-time monitoring solution for PCBs in soil samples or other samples.
A Study on the Optimization of the Conductive Ball Manufacturing Process, Used for Anisotropic Conductive Films
Authors: Choi Jong-Keun , Kim Young-Gyun , Han Kwan-Young
Published: August 2022

Currently, as the next-generation of display progresses—with high performance and high integration—the surface mounting technology of components is very important. In particular, in the case of flexible displays, such as rollable and bendable displays, ACF that connects wires to any curvature is essential. However, the conductive ball used inside the ACF has had problems with particle size and non-uniform metal coating. It was confirmed that the presence of solvent and oxygen, which are used in polymer synthesis, affects the sphere formation of polymer beads. By optimizing the factors affecting the polymer beads, a perfect spherical polymer bead was manufactured. In addition, the conductive ball manufacturing process was optimized by confirming the factors affecting the metal coating. The metal coating on the surface of the polymer bead was applied with a uniform thickness by considering the specific surface area and concentration of the conductive balls, and, through this optimized process, conductive balls for anisotropic conductive films with uniform size and metal thickness were obtained.
VGG-C Transform Model with Batch Normalization to Predict Alzheimer’s Disease through MRI Dataset
Authors: Tuvshinjargal Batzaya , Hwang Heejoung
Published: August 2022

Alzheimer’s disease is the most common cause of dementia and is a generic term for memory and other cognitive abilities that are severe enough to interfere with daily life. In this paper, we propose an improved prediction method for Alzheimer’s disease using a quantization method that transforms the MRI data set using a VGG-C Transform model and a convolutional neural network (CNN) consisting of batch normalization. MRI image data of Alzheimer’s disease are not fully disclosed to general research because it is data from real patients. So, we had to find a solution that could maximize the core functionality in a limited image. In other words, since it is necessary to adjust the interval, which is an important feature of MRI color information, rather than expressing the brain shape, the brain texture dataset was modified in the quantized pixel intensity method. We also use the VGG family, where the VGG-C Transform model with bundle normalization added to the VGG-C model performed the best with a test accuracy of about 0.9800. However, since MRI images are 208 × 176 pixels, conversion to 224 × 224 pixels may result in distortion and loss of pixel information. To address this, the proposed VGG model-based architecture can be trained while maintaining the original MRI size. As a result, we were able to obtain a prediction accuracy of 98% and the AUC score increased by up to 1.19%, compared to the normal MRI image data set. It is expected that our study will be helpful in predicting Alzheimer’s disease using the MRI dataset.
Surface Defect Detection of Strip-Steel Based on an Improved PP-YOLOE-m Detection Network
Authors: Zhang Yang , Liu Xiaofang , Guo Jun , Zhou Pengcheng
Published: August 2022

Surface-defect detection is crucial for assuring the quality of strip-steel manufacturing. Strip-steel surface-defect detection requires defect classification and precision localization, which is a challenge in real-world applications. In this research, we propose an improved PP-YOLOE-m network for detecting strip-steel surface defects. First, data augmentation is performed to avoid the overfitting problem and to improve the model’s capacity for generalization. Secondly, Coordinate Attention is embedded in the CSPRes structure of the backbone network to improve the backbone network’s feature extraction capabilities and obtain more spatial location information. Thirdly, Spatial Pyramid Pooling is specifically replaced for the Atrous Spatial Pyramid Pooling in the neck network, enabling the multi-scale network to broaden its receptive field and gain more information globally. Finally, the SIoU loss function more accurately calculates the regression loss over GIoU. Experimental results show that the improved PP-YOLOE-m network’s AP, AP50, and AP75, respectively, achieved 44.6%, 80.3%, and 45.3% for strip-steel surface defects detection on the NEU-DET dataset and improved by 2.2%, 4.3%, and 4.6% over the PP-YOLOE-m network. Further, our method has fast and real-time detection capabilities and can run at 95 FPS on a single Tesla V100 GPU.
“Follower of the Reference Point”: Platform Utility-Oriented Incentive Mechanism in Crowdsensing
Authors: Peng Runze , Huang Wei , Xu Hucheng , Pi Mingyang , Liu Jiaqi
Published: August 2022

Crowdsensing uses the sensing units of many participants with idle resources to collect data. Since the budget of the platform is limited, it is crucial to design a mechanism to motivate participants to lower their bids. Current incentive mechanisms assume that participants’ gains and losses are absolute, but behavioral economics shows that a certain reference point determines participants’ gains and losses. Reference dependence theory shows that the reference reward given by a platform and the reward obtained before will greatly affect the decision-making of the participant. Therefore, this paper proposes a participants’ decision-making mechanism based on the reference dependence theory. We set a reference point to reduce the participants’ bids, improving the platform’s utility. At the same time, risk preference reversal theory shows that participants evaluate the benefits based on the relative value of the rewards rather than the absolute value. Therefore, this paper proposes a winner selection mechanism based on the risk preference reversal theory. Theoretical analysis and simulations demonstrate that this paper’s incentive mechanism can guarantee the platform’s utility and improve the task completion rate.
Differences between Yaks and Qaidam Cattle in Digestibilities of Nutrients and Ruminal Concentration of Volatile Fatty Acids Are not Dependent on Feed Level
Authors: Liu Hu , Wu Daozhicairang , Degen Abraham Allan , Hao Lizhuang , Gan Shuiyan , Liu Hongshan , Cao Xuliang , Zhou Jianwei , Long Ruijun
Published: August 2022

The Qinghai–Tibetan Plateau (QTP) is characterized by highly fluctuating seasonal pastures. Yaks (Bos grunniens) graze at higher altitudes than Qaidam cattle (Bos taurus), but the two bovine species co-graze in their overlapping ranges. We hypothesized that yaks would digest nutrients to a greater extent and utilize energy more efficiently than cattle at low dietary intakes, but the difference between bovine species would not be apparent at high intakes. To test this hypothesis, six yaks (203 ± 6.0 kg) and six Qaidam cattle (214 ± 9.0 kg), all 3.5-year-old castrated males, were used in two concurrent 4 × 4 Latin square designs with two extra steers of each species in each period. The digestibilities of dry matter, organic matter, crude protein, ether extract, neutral and acid detergent fiber were greater (p < 0.05) in yaks than in cattle and decreased linearly (p < 0.05) when feed level (FL) increased. The average daily gain (ADG), the ratios of digestible energy (DE) to gross energy and metabolizable energy (ME) to DE, and ruminal total volatile fatty acids and ammonia-N concentrations were greater (p < 0.001) in yaks than in cattle and increased linearly (p < 0.001) when FL increased. Based on the regression equations of ADG on ME intake, the daily ME requirement for maintenance in yaks was 0.53 MJ BW−0.75 d−1, which was lesser (p < 0.05) than the 0.62 MJ BW−0.75 d−1 in cattle. We concluded that: (1) when differences between breeds emerged, the differences existed for all FLs; (2) maintenance energy requirement was lesser and ADG was greater in yaks than in cattle; (3) the digestibilities of nutrients were greater in yaks than in cattle when consuming only oat hay pellets. These findings indicate that yaks adapt to fluctuating dietary intakes in harsh environments by having a low energy requirement and high digestibility of nutrients, independent of the FL.
Responses of Fermentation Characteristics and Microbial Communities to Vitamin B12 Supplementation in In Vitro Ruminal Cultures
Authors: Wang Kun , Liu Zihao , Du Chunmei , Xiong Benhai , Yang Liang
Published: August 2022

Vitamin B12, an important cofactor involved in propionate formation, is synthesized exclusively by bacteria and archaebacteria. Humans need to intake vitamin B12 through food, and dairy products are generally the best source of vitamin B12. In the present study, the effects of vitamin B12 supplementation in diets on in vitro ruminal fermentation characteristics and microbial communities were investigated to provide a reference for increasing the vitamin B12 content in milk by dietary supplementation. A completely randomized design was carried out using the in vitro rumen culture technique, and 5 vitamin B12 dose levels (0, 0.5, 1.0, 2.0, and 4 mg/g of dry matter) were used. The results showed that vitamin B12 supplementation in diets decreased acetate: propionate ratio and butyrate concentration. The change in the acetate: propionate ratio can be attributed to the increased relative abundances of the Proteobacteria phylum and the Negativicutes class, both of which are involved in propionate metabolism. The decrease in butyrate concentration can likely be attributed to a reduction in relative abundance of species belonging to the Clostridia class, which are known as the predominant butyrate producers in the mammalian intestine. In addition, vitamin B12 supplementation in diets reduced the CH4 production by altering the species composition of the archaeal community. In conclusion, dietary supplementation of vitamin B12 resulted in rumen perturbation. In vivo studies should be conducted cautiously when evaluating the effects of vitamin B12 supplementation on the synthesis and absorption of it, as well as its content in milk.
Selection of Yeast and Lactic Acid Bacteria Strains, Isolated from Spontaneous Raw Milk Fermentation, for the Production of a Potential Probiotic Fermented Milk
Authors: Galli Viola , Venturi Manuel , Mari Eleonora , Guerrini Simona , Granchi Lisa
Published: August 2022

Probiotic milk is a class of fermented milk that possesses health-promoting effects, not only due to the lactic acid bacteria (LAB) presence but potentially also to yeast activity. Hence, the aim of this work was to isolate and select yeasts from spontaneous milk fermentations to be used as inoculum, together with LAB, for manufacturing a potentially probiotic acidic low-alcohol fermented milk. Six yeast species were detected from the spontaneous milk fermentation. A screening of 13 yeast strains and 14 previously isolated LAB strains, based on the resistance to bile salts and to acidic conditions, was carried out. The best performing strains were successively tested for in vitro gastrointestinal tolerance. A strain of Kluyveromyces marxianus and a strain of Lactococcus lactis were selected for the manufacturing of two different fermented milk. The values of the main technological and microbiological parameters (pH, organic acids, ethanol, and microbial concentrations) of the experimental milk were in the range of those reported for this category of products. The evaluation of microorganism survival in fermented milk samples subjected to simulated gastrointestinal conditions highlighted a high resistance of both strains. In conclusion, the selected microbial starter culture enabled the setting up of potential probiotic fermented milk.
Apple Pomace Modulates the Microbiota and Increases the Propionate Ratio in an In Vitro Piglet Gastrointestinal Model
Authors: Dufourny Sandrine , Lebrun Sarah , Douny Caroline , Dubois Benjamin , Scippo Marie-Louise , Wavreille José , Rondia Pierre , Everaert Nadia , Delcenserie Véronique
Published: August 2022

Apple pomace (AP) contains biomolecules that induce changes in intestinal fermentation of monogastrics with positive expected health effects. The weaning of piglets can induce economic losses due to intestinal disturbances; new weaning strategies are, thus, welcome. The purpose of this study was to test the effect of AP on fermentation products by using baby-SPIME, an in vitro multi-compartment model dedicated to piglet weaning. A comparison was done on short chain fatty acid (SCFA) ratio and the microbiota induced in bioreactors between a control culture medium vs. an AP culture medium. The results of 2 preliminary runs showed that AP medium increased the molar ratio of propionate (p = 0.021) and decreased the molar ratio of butyrate (p = 0.009). Moreover, this medium increased the cumulative relative abundance of Prevotella sp. and Akkermansia sp. in bioreactors. AP could promote an ecosystem enriched with bacteria known as next-generation probiotics (NGP)—likely influencing the energy metabolism of piglets by their fermentation metabolites. AP could be used as a dietary strategy to influence bacterial changes in the intestine by stimulating the growth of bacteria identified as NGP.
Comparative Transcriptome Analysis Reveals Gene Expression Differences in Eggplant (Solanum melongena L.) Fruits with Different Brightness
Authors: Zhang Aidong , Huang Qianru , Li Jianyong , Zhu Weimin , Liu Xiaohui , Wu Xuexia , Zha Dingshi
Published: August 2022

Fruit brightness is an important quality trait that affects the market value of eggplant. However, few studies have been conducted on eggplant brightness. In this study, we aimed to identify genes related to this trait in three varieties of eggplant with different fruit brightness between 14 and 22 days after pollination. Using RNA-Seq Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses, we found that wax- and cutin-related pathways and differentially expressed genes displayed significant differences among different development stages and varieties. Scanning electron microscopy revealed that the wax layer was thinner in ‘30-1’ and ‘QPCQ’ than in ‘22-1’. Gas chromatography-mass spectrometry analysis revealed that wax content was significantly lower in ‘30-1’ than in ‘22-1’, which indicated that wax may be an important factor determining fruit brightness. We further identified and analyzed the KCS gene family, which encodes the rate-limiting enzyme of FA elongation in wax synthesis. The results provide an insight into the molecular mechanisms of fruit brightness in eggplants and further eggplant breeding programs.
Impact of Guar Gum and Locust Bean Gum Addition on the Pasting, Rheological Properties, and Freeze–Thaw Stability of Rice Starch Gel
Authors: Xu Xuejiao , Ye Shuhui , Zuo Xiaobo , Fang Sheng
Published: August 2022

Improving the gel texture and stability of rice starch (RS) by natural hydrocolloids is important for the development of gluten-free starch-based products. In this paper, the effects of guar gum and locust bean gum on the pasting, rheological properties, and freeze–thaw stability of rice starch were investigated by using a rapid visco analyzer, rheometer, and texture analyzer. Both gums can modify the pasting properties, revealed by an increment in the peak, trough, and final viscosities, and prevent the short-term retrogradation tendency of RS. Dynamic viscoelasticity measurements also indicated that the starch–gum system exhibits superior viscoelastic properties compared with starch alone, as revealed by its higher storage modulus (G′). Compared with the control, the hysteresis loop area of the guar gum-containing system and locust bean gum-containing system was reduced by 37.7% and 24.2%, respectively, indicating that the addition of gums could enhance shear resistance and structure recovery properties. The thermodynamic properties indicated that both gums retard short-term retrogradation as well as long-term retrogradation of the RS gels. Interestingly, the textural properties and freeze–thaw stability of the RS gel were significantly improved by the addition of galactomannans (p < 0.05), and guar gum was more effective than locust bean gum, which may be due to the different mannose to galactose ratio. The results provide alternatives for gluten-free recipes with improved texture properties and freeze–thaw stability.
Pilot-Scale Vinification of Cabernet Sauvignon Using Combined Lactiplantibacillus plantarum and Saccharomyces cerevisiae to Achieve Wine Acidification
Authors: Jiang Jiao , Zhang Wenjing , Wu Yitian , Shi Xuerong , Yang Xiaobing , Song Yuyang , Qin Yi , Ye Dongqing , Liu Yanlin
Published: August 2022

Insufficient acidity in grape berries from warm climate regions has been exacerbated due to global warming, thereby becoming a major concern for winemaking. The wine lactic acid bacterium Lactiplantibacillus plantarum has potential to ameliorate wine acidity by producing lactic acid from hexose metabolism, but its impact on wine compositions and sensory outcomes is not well studied. Here, we evaluated acidification and fermentation performance of indigenous L. plantarum in two inoculation regimes (i.e., reverse inoculation and co-inoculation) by conducting pilot-scale vinification using Cabernet Sauvignon with low acidity. Important parameters of the bio-acidified wines, including fermentation kinetics, basic oenological parameters, volatile and sensory profile were compared to those in wines produced by single Saccharomyces cerevisiae with/without chemical acidification. Total titratable acidity in L. plantarum wines were either comparable or significantly higher compared to the chemical acidification control. Chemical profiling reviewed remarkable differences in certain organic acids and major volatile compounds, especially an up to a five-fold, six-fold, and nine-fold increase in lactic acid, ethyl lactate and isoamyl lactate, respectively. Changes in chemical compositions of the bio-acidified wines resulted in differentiated sensory perception compared to the control wines. Except having higher scores for “wine acidity”, the flavour profile of the bio-acidified wines was shifted towards “jammy fruit” and “butter” aromas. Together, these findings highlighted the applicability of using L. plantarum to induce biological acidification along with modulation of wine flavour.
Effect of Monosodium Glutamate on Salt and Sugar Content Reduction in Cooked Foods for the Sensory Characteristics and Consumer Acceptability
Authors: Chung Yehji , Yu Daeung , Kwak Han Sub , Park Sung-Soo , Shin Eui-Cheol , Lee Youngseung
Published: August 2022

Three different types of typical Korean foods were studied to investigate the effect of monosodium glutamate (MSG) on the sensory characteristics and hedonic perception of sodium- or sugar-reduced samples. The first consumer test (n = 300) was conducted to evaluate the overall liking (OL) of the samples containing four different levels of salt and sugar contents without added MSG, while the second consumer test (n = 300) was designed to examine the effects of MSG on the samples containing reduced salt and sugar contents with the lowest observed OL in the first consumer test. The results showed that the intensity of the umami taste and saltiness of the samples increased, whereas sourness and bitterness were suppressed with added MSG. The samples with the lowest salt contents were observed to be acceptable to consumers after MSG addition, indicating a 23% reduction in sodium intake. Bitterness was partially masked, while sweetness, umami taste, and saltiness were the main factors affecting the OL after MSG addition. However, no consistent results of added MSG on the sensory attributes of samples with reduced sugar contents were observed, possibly indicating that the effect of umami taste on sweetness depends on the MSG concentration used or the type of food studied. This study could be beneficial to researchers who want to know the optimal level of MSG required to reduce the sodium or sugar contents in cooked food.
Antioxidant Capacity and Profiles of Phenolic Acids in Various Genotypes of Purple Wheat
Authors: Shamanin Vladimir P. , Tekin-Cakmak Zeynep H. , Gordeeva Elena I. , Karasu Salih , Pototskaya Inna , Chursin Alexandr S. , Pozherukova Violetta E. , Ozulku Gorkem , Morgounov Alexey I. , Sagdic Osman , Koksel Hamit
Published: August 2022

The total phenolic content, phenolic compositions, and antioxidant capacity in the grain of 40 purple wheat genotypes were studied. In this study, purple wheats were investigated in terms of their composition of free and bound phenolic acids and 2,2-diphenyl-1-picrylhydrazyl radical scavenging capacity. The free phenolic content ranged from 164.25 to 271.05 mg GAE/100 g DW and the bound phenolic content was between 182.89–565.62 mg GAE/100 g wheat. The total phenolic content of purple wheat samples ranged from 352.65 to 771.83 mg GAE/100 g wheat. Gallic acid, protocatechuic acid, catechin, 4-hydroxybenzoic acid, syringic acid, ellagic acid, m-coumaric acid, o-coumaric acid, chrysin, caffeic acid, p-coumaric acid, ferulic acid, quercetin, kaempferol, rutin, sinapic acid, and chlorogenic acid were detected by HPLC system. Gallic acid, benzoic acid derivatives, and dominant phenolics, which are frequently found in cereals, were also dominant in purple wheat samples and were found in free fractions. The antioxidant capacity was assessed using the DPPH method. The antioxidant capacity (AA%) in the free phenolic extracts of the purple wheats was between 39.7% and 59.5%, and the AA% values of bound phenolic extract of the purple wheat varied between 42.6% and 62.7%. This study suggested that purple wheat samples have high phenolic compound content as antioxidant potential and therefore consumption of purple wheat-containing food products may provide health benefits.
Comparison of Volatile Compounds Contributing to Flavor of Wild Lowbush (Vaccinium augustifolium) and Cultivated Highbush (Vaccinium corymbosum) Blueberry Fruit Using Gas Chromatography-Olfactometry
Authors: Forney Charles F. , Qiu Songshan , Jordan Michael A. , McCarthy Dylan , Fillmore Sherry
Published: August 2022

The flavor of blueberry fruit products is an important parameter determining consumer satisfaction. Wild lowbush blueberries are primarily processed into products, but their flavor chemistry has not been characterized. The objective of this study was to characterize the aroma chemistry of lowbush blueberries and compare it with that of highbush. Aroma volatiles of lowbush blueberries from four Canadian provinces and five highbush blueberry cultivars were isolated using headspace solid-phase microextraction (SPME) and characterized using gas chromatography-olfactometry (GC-O) and 2-dimensional gas chromatography-time of flight-mass spectrometry (GC×GC-TOF-MS). Lowbush fruit volatiles were composed of 48% esters, 29% aldehydes and 4% monterpenoids compared to 48% aldehydes, 26% monoterpenoids and 3% esters in highbush fruit. Twenty-three aroma-active peaks were identified in lowbush compared to forty-two in highbush fruit using GC-O. The most aroma-active compounds in lowbush fruit were ethyl 2-methylbutanoate, methyl 2-methylbutanoate, methyl 3-methylbutanoate, ethyl 3-methylbutanoate and ethyl propanoate compared to geraniol, (Z)-3-hexen-1-ol, 1-octen-3-one, α-terpineol and linalool in highbush fruit. The aroma volatile composition was more consistent among lowbush fruit samples than the five highbush cultivars. Aroma-active GC-O peaks were described more frequently as “floral”, “fruity”, “sweet” and “blueberry” in lowbush than in highbush fruit. Results suggest wild lowbush blueberries would provide “fruitier” and “sweeter” flavors to food products than cultivated highbush fruit.
Insight into the Gel Properties of Antarctic Krill and Pacific White Shrimp Surimi Gels and the Feasibility of Polysaccharides as Texture Enhancers of Antarctic Krill Surimi Gels
Authors: Li Shuang , Lin Songyi , Jiang Pengfei , Bao Zhijie , Li Sibo , Sun Na
Published: August 2022

Antarctic krill is a potential and attractive resource for consumption. However, most Antarctic krill meat is used to produce primary products with low commercial value, with few highly processed products. This study aimed to evaluate and improve the gelling properties of Antarctic krill surimi, with Pacific white shrimp surimi as control. Compared with Pacific white shrimp surimi, the lower β-sheet content and protein aggregation degree had a severe impact on the formation of the gel network of Antarctic krill surimi, which resulted in weaker breaking force, gel strength, and viscoelasticity (p < 0.05). Moreover, water retention capacity and molecular forces had a positive effect on the stability of the gel matrix of shrimp surimi. Thus, the high α-helix/β-sheet ratio, weak intermolecular interactions, and low level of protein network cross-linkage were the main reasons for the poor quality of Antarctic krill surimi. On this basis, the effects of six polysaccharides on the texture properties of Antarctic krill surimi were studied. Chitosan, konjac glucomannan, sodium carboxyl methyl cellulose, and waxy maize starch resulted in no significant improvement in the texture properties of Antarctic krill surimi (p > 0.05). However, the addition of ι-carrageenan (2%) or κ-carrageenan (1~2%) is an effective way to improve the texture properties of Antarctic krill surimi (p < 0.05). These findings will contribute to the development of reconstituted Antarctic krill surimi products with high nutritional quality and the promotion of deep-processing products of Antarctic krill meat.
Valorization of Green Biomass: Alfalfa Pulp as a Substrate for Oyster Mushroom Cultivation
Authors: Zhou Fa , Hansen Mikkel , Hobley Timothy John , Jensen Peter Ruhdal
Published: August 2022

In this study, the potential of alfalfa pulp as an alternative substrate to wheat straw for the cultivation of oyster mushroom (Pleurotus ostreatus) was investigated. The major components associated with different mushroom stages were evaluated, as well as changes in lignocellulolytic enzyme activities in substrates composed of alfalfa pulp, wheat straw or a combination of both. Based on the results, alfalfa pulp was demonstrated to be a better substrate than wheat straw for the production of oyster mushrooms, with a high biological efficiency of 166.3 ± 25.4%. Compared to the cultivation period on commercial straw (31 days), a shorter lifecycle for oyster mushroom was found on alfalfa pulp (24 days), which could help to reduce the risk of contamination during industrial production. Study of the spent substrate as well as the harvested mushrooms revealed that the biological efficiency was related to the higher protein content (17.42%) in the alfalfa pulp compared to wheat straw, as well as greater degradation of cellulose (57.58%) and hemicellulose (56.60%). This was, by and large, due to greater extracellular hydrolytic and oxidative enzyme activity from the mushroom growth in the alfalfa pulp. The quality and safety of the fruiting bodies produced on alfalfa pulp was evaluated, which showed that the protein content was 20.4%, of which 46.3% was essential amino acids, and levels of trace elements and heavy metals were below acceptable limits. Hence, oyster mushroom cultivation using alfalfa pulp provides an alternative method to produce a value-added product, while reducing the biomass wastes in the green protein bio-refinery, and may contribute to sustainable growth in the agricultural industry.
An Exploratory Critical Review on TNF-α as a Potential Inflammatory Biomarker Responsive to Dietary Intervention with Bioactive Foods and Derived Products
Authors: Quarta Stefano , Massaro Marika , Carluccio Maria Annunziata , Calabriso Nadia , Bravo Laura , Sarria Beatriz , García-Conesa María-Teresa
Published: August 2022

This review collects and critically examines data on the levels of tumour necrosis factor-alpha (TNF-α) in lean, overweight and obese subjects, and the effects of intervention with different foods and food products containing bioactive constituents in overweight/obese individuals. We additionally explore the influence of different single nucleotide polymorphisms (SNPs) on TNF-α levels and compare the response to food products with that to some anti-obesity drugs. Our aim was to provide an overview of the variability, consistency, and magnitude of the reported effects of dietary factors on TNF-α, and to envisage the reliability of measuring changes in the levels of this cytokine as a biomarker responsive to food intervention in association with the reduction in body weight. Regarding the circulating levels of TNF-α, we report: (i) a large intra-group variability, with most coefficients of variation (CV%) values being ≥30% and, in many cases, >100%; (ii) a large between-studies variability, with baseline TNF-α values ranging from <1.0 up to several hundred pg/mL; (iii) highly variable effects of the different dietary approaches with both statistically significant and not significant decreases or increases of the protein, and the absolute effect size varying from <0.1 pg/mL up to ≈50 pg/mL. Within this scenario of variability, it was not possible to discern clear differentiating limits in TNF-α between lean, overweight, and obese individuals or a distinct downregulatory effect on this cytokine by any of the different dietary approaches reviewed, i.e., polyunsaturated fatty acids (PUFAs), Vitamin-D (VitD), mixed (micro)nutrients, (poly)phenols or other phytochemicals. Further, there was not a clear relationship between the TNF-α responses and body weight changes. We found similarities between dietary and pharmacological treatments in terms of variability and limited evidence of the TNF-α response. Different factors that contribute to this variability are discussed and some specific recommendations are proposed to reinforce the need to improve future studies looking at this cytokine as a potential biomarker of response to dietary approaches.
Best Proximity Point Theorems for the Generalized Fuzzy Interpolative Proximal Contractions
Authors: Javed Khalil , Lashin Maha M. A. , Nazam Muhammad , Al Sulami Hamed H. , Hussain Aftab , Arshad Muhammad
Published: August 2022

The idea of best proximity points of the fuzzy mappings in fuzzy metric space was intorduced by Vetro and Salimi. We introduce a new type of proximal contractive condition that ensures the existence of best proximity points of fuzzy mappings in the fuzzy complete metric spaces. We establish certain best proximity point theorems for such proximal contractions. We improve and generalize the fuzzy proximal contractions by introducing Ψ,Φ-fuzzy proximal contractions and Ψ,Φ-fuzzy proximal interpolative contractions. The obtained results improve and generalize many best proximity point theorems published earlier. Moreover, we provide many nontrivial examples to validate our best proximity point theorem.
Fractal Analysis and Time Series Application in ZY-4 SEM Micro Fractographies Evaluation
Authors: Paun Maria-Alexandra , Paun Vladimir-Alexandru , Paun Viorel-Puiu
Published: August 2022

SEM microfractographies of Zircaloy-4 are studied by fractal analysis and the time-series method. We first develop a computer application that associates the fractal dimension and lacunarity to each SEM micrograph picture, and produce a nonlinear analysis of the data acquired from the quantitatively evaluated time series. Utilizing the phase space-embedding technique to reconstruct the attractor and to compute the autocorrelation dimension, the fracture surface of the Zircaloy-4 samples is investigated. The fractal analysis method manages to highlight damage complications and provide a description of morphological parameters of various fractures by calculating the fractal dimension and lacunarity.
Fibrous and Spherical Aggregates of Ovotransferrin as Stabilizers for Oleogel-Based Pickering Emulsions: Preparation, Characteristics and Curcumin Delivery
Authors: Zhou Qi , Wei Zihao , Xu Yanan , Xue Changhu
Published: August 2022

This study aimed to explore the effects and mechanisms of differently shaped aggregates of ovotransferrin (OVT) particles on oleogel-based Pickering emulsions (OPEs). Medium-chain triglyceride oil-based oleogels were constructed using beeswax, and their gel-sol melting temperatures were investigated. Atomic force microscopy confirmed that both OVT fibrils and OVT spheres were successfully prepared, and the three-phase contact angle measurements indicated that fibrous and spherical aggregates of OVT particles possessed great potential to stabilize the OPEs. Afterward, the oil-in-water OPEs were fabricated using oleogel as the oil phase and OVT fibrils/spheres as the emulsifiers. The results revealed that OPEs stabilized with OVT fibrils (FIB-OPEs) presented a higher degree of emulsification, smaller droplet size, better physical stability and stronger apparent viscosity compared with OPEs stabilized with OVT spheres (SPH-OPEs). The freeze–thaw stability test showed that the FIB-OPEs remained stable after three freeze–thaw cycles, while the SPH-OPEs could barely withstand one freeze–thaw cycle. An in vitro digestion study suggested that OVT fibrils conferred distinctly higher lipolysis (46.0%) and bioaccessibility (62.8%) of curcumin to OPEs.
Gel Carriers for Plant Extracts and Synthetic Pesticides in Rodent and Arthropod Pest Control: An Overview
Authors: Shah Jawad Ali , Vendl Tomas , Aulicky Radek , Frankova Marcela , Stejskal Vaclav
Published: August 2022

Insecticides and rodenticides form the basis of integrated pest management systems worldwide. As pest resistance continues to increase and entire groups of chemical active ingredients are restricted or banned, manufacturers are looking for new options for more effective formulations and safer application methods for the remaining pesticide ingredients. In addition to new technological adaptations of mainstream formulations in the form of sprays, fumigants, and dusts, the use of gel formulations is becoming increasingly explored and employed. This article summarizes information on the current and potential use of gel (including hydrogel) and paste formulations against harmful arthropods or rodents in specific branches of pest management in the agricultural, food, stored product, structural wood, urban, medical, and public health areas. Due to the worldwide high interest in natural substances, part of the review was devoted to the use of gels for the formulation of pesticide substances of botanical origin, such as essential or edible oils. Gels as emerging formulation of so called “smart insecticides” based on molecular iRNA disruptors are discussed.
Polysaccharide-Based Edible Gels as Functional Ingredients: Characterization, Applicability, and Human Health Benefits
Authors: Pascuta Mihaela Stefana , Varvara Rodica-Anita , Teleky Bernadette-Emőke , Szabo Katalin , Plamada Diana , Nemeş Silvia-Amalia , Mitrea Laura , Martău Gheorghe Adrian , Ciont Călina , Călinoiu Lavinia Florina , Barta Gabriel , Vodnar Dan Cristian
Published: August 2022

Nowadays, edible materials such as polysaccharides have gained attention due to their valuable attributes, especially gelling property. Polysaccharide-based edible gels (PEGs) can be classified as (i) hydrogels, (ii) oleogels and bigels, (iii) and aerogels, cryogels and xerogels, respectively. PEGs have different characteristics and benefits depending on the functional groups of polysaccharide chains (e.g., carboxylic, sulphonic, amino, methoxyl) and on the preparation method. However, PEGs are found in the incipient phase of research and most studies are related to their preparation, characterization, sustainable raw materials, and applicability. Furthermore, all these aspects are treated separately for each class of PEG, without offering an overview of those already obtained PEGs. The novelty of this manuscript is to offer an overview of the classification, definition, formulation, and characterization of PEGs. Furthermore, the applicability of PEGs in the food sector (e.g., food packaging, improving food profile agent, delivery systems) and in the medical/pharmaceutical sector is also critically discussed. Ultimately, the correlation between PEG consumption and polysaccharides properties for human health (e.g., intestinal microecology, “bridge effect” in obesity, gut microbiota) are critically discussed for the first time. Bigels may be valuable for use as ink for 3D food printing in personalized diets for human health treatment. PEGs have a significant role in developing smart materials as both ingredients and coatings and methods, and techniques for exploring PEGs are essential. PEGs as carriers of bioactive compounds have a demonstrated effect on obesity. All the physical, chemical, and biological interactions among PEGs and other organic and inorganic structures should be investigated.
Teledentistry as an Effective Tool for the Communication Improvement between Dentists and Patients: An Overview
Authors: Islam Md Refat Readul , Islam Rafiqul , Ferdous Sultana , Watanabe Chiharu , Yamauti Monica , Alam Mohammad Khursheed , Sano Hidehiko
Published: August 2022

Teledentistry is an online dental care service that allows patients and dentists to meet in real time, safely, without being at the same location. During the COVID-19 pandemic, real-time videoconferencing has gained popularity in the field of teledentistry, with numerous benefits for both patients and dentists. Online consultations can minimize costs, maximize time, and provide more convenient care options for both patients and dentists. When practicing teledentistry, a dentist must establish a good doctor–patient relationship. Dentists must ensure that the telecommunication solution that they choose meets their clinical requirements and complies with privacy laws. Dentists should provide adequate information to patients about the limitations, advantages, and disadvantages that may occur during online consultation. Dentists must follow guidelines and procedures regarding informed consent, patient details, personal communications, and consultancies’ privacy and confidentiality. The patient should be aware of the limitations of teledentistry, and dentists will provide the best advice possible in the absence of a face-to-face consultation. This article discusses how teledentistry could be an effective tool for dentists and patients.
Impact of Paleoclimatic Changes on the Cultural and Historical Processes at the Turn of the Late Bronze—Early Iron Ages in the Northern Black Sea Region
Authors: Kulkova Marianna A. , Kashuba Maya T. , Agulnikov Sergey M. , Kulkov Alexander M. , Streltsov Mikhail A. , Vetrova Maria N. , Zanoci Aurel
Published: August 2022

The Late Bronze Age crisis is one of the most significant events in human history an had occurred in about 1200 BCE. The aridization was one the reasons of a decline of agriculture, migrant expansion and the transition to nomadic style of life. In Eastern Mediterranean the collapse of the advanced civilizations such as the Mycenaean, Hittite, Canaanite, Akkadian occurred in this time. The reconstruction of cultural-historical processes at the turn of the Bronze-Early Iron Ages and environment during this “critical” period of 13th–9th centuries BCE in the Northern Black Sea region is important for understanding this event. Interdisciplinary investigations of the paleoclimatic reconstructions and the cultural traditions have been carried out at the key archaeological sites located in the North-Western Pontic region (Saharna Mică, Saharna Mare, Glinjeni II-La Șanț, Dikiy Sad sites and Cazaclia necropolis). For reconstruction of paleoclimatic conditions and anthropogenic activity, the methods of geochemical indication of paleoclimatic conditions and radiocarbon dating were applied. The climatic changes in the Dniester basin towards aridization around 11th–9th century’s calBC were a crisis of the Belozerkа culture in the Pontic steppe and the trigger for the spreading of the Cozia-Saharna cultural communities into the forest-steppe zone and the formation of fortified settlements the Saharna Miča, the Saharna Mare and the Glinjeni II-La Șanț.
Chemical Composition of Combretum erythrophyllum Leaf and Stem Bark Extracts
Authors: Bantho Sahejna , Naidoo Yougasphree , Dewir Yaser Hassan , Bantho Ayuvna , Murthy Hosakatte Niranjana
Published: August 2022

Combretaceae is a large Angiosperm family that is highly sought after because of its pronounced medicinal value. Combretum is recognized as the largest genus, prevalent in southern Africa due to its extensive use in traditional medicine. This study aimed to provide a comparative analysis of the phytochemical constituents of the leaf and stembark extracts of Combretum erythrophyllum (Burch.) Sond. Leaf and stembark crude extracts were generated using hexane, chloroform, and methanol as the solvents of choice. Qualitative phytochemical tests indicated the presence of phytocompounds, including carbohydrates, alkaloids, sterols, phenols, fixed oils, and fats. Flavonoids were found within the leaf extracts only, while saponins, mucilage, and gums were specifically identified within the stembark extracts. The first reported gas chromatography–mass spectrometry (GC–MS) screening of C. erythrophyllum leaf and stembark extracts was conducted, yielding the identification of 266 phytocompounds. Major phytocompounds such as sitosterol and lupeol, which may have possible anti-cancer and anti-inflammatory properties, were identified. Furthermore, a pharmacogenetic evaluation was conducted. As a result, both the leaf and stem bark material were seen to fluoresce a wide array of colors (brown, red, green, and blue colorations), indicating the presence of beneficial phytometabolites and their use in medicinal applications. Given the wide array of proposed medicinal benefits associated with the presence of phytocompounds identified within C. erythrophyllum, this species should be considered for its medicinal importance. The isolation and extraction of these beneficial compounds open further avenues for their use in the pharmaceutical industry.
Spontaneous Ignition of Cryo-Compressed Hydrogen in a T-Shaped Channel System
Authors: Cirrone Donatella , Makarov Dmitriy , Molkov Vladimir
Published: August 2022

Sudden releases of pressurised hydrogen may spontaneously ignite by the so-called “diffusion ignition” mechanism. Several experimental and numerical studies have been performed on spontaneous ignition for compressed hydrogen at ambient temperature. However, there is no knowledge of the phenomenon for compressed hydrogen at cryogenic temperatures. The study aims to close this knowledge gap by performing numerical experiments using a computational fluid dynamics model, validated previously against experiments at atmospheric temperatures, to assess the effect of temperature decrease from ambient 300 K to cryogenic 80 K. The ignition dynamics is analysed for a T-shaped channel system. The cryo-compressed hydrogen is initially separated from the air in the T-shaped channel system by a burst disk (diaphragm). The inertia of the burst disk is accounted for in the simulations. The numerical experiments were carried out to determine the hydrogen storage pressure limit leading to spontaneous ignition in the configuration under investigation. It is found that the pressure limit for spontaneous ignition of the cryo-compressed hydrogen at temperature 80 K is 9.4 MPa. This is more than 3 times larger than pressure limit for spontaneous ignition of 2.9 MPa in the same setup at ambient temperature of 300 K.
Osteopontin in Cancer: Mechanisms and Therapeutic Targets
Authors: Kariya Yoshinobu , Kariya Yukiko
Published: August 2022

Despite significant advances in the understanding of cancer biology, cancer is still a leading cause of death worldwide. Expression of the tumor microenvironment component, osteopontin, in tumor tissues, plasma, and serum, has been shown to be associated with a poor prognosis and survival rate in various human cancers. Recent studies suggest that osteopontin drives tumor development and aggressiveness using various strategies. In this review, we first provide an overview of how osteopontin promotes tumor progression, such as tumor growth, invasion, angiogenesis, and immune modulation, as well as metastasis and chemoresistance. Next, we address how the functional activities of osteopontin are modulated by the interaction with integrins and CD44 receptors, but also by the post-translational modification, such as proteolytic processing by several proteases, phosphorylation, and glycosylation. Then, we review how osteopontin activates tumor-associated macrophages (TAMs) and cancer-associated fibroblasts (CAFs), and functions as an immunosuppressor by regulating immune surveillance and immune checkpoint in the tumor microenvironment. Finally, we discuss the potential applications of osteopontin as a biomarker and as a therapeutic target.