bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of Long-Term Fertilisation of Potato Starch Wastewater on the Growth of Scots Pines: A Retrospective Analysis
Authors: Chojnacka-Ożga Longina , Lendzion Jerzy , Ożga Wojciech
Published: September 2022

The article discusses the impact of the application of potato starch wastewater as a fertiliser on the growth responses of Scots pines at the Forest Wastewater Treatment Plant (FWTP) in Iława. More specifically, our study sought to determine the direction, extent, and duration of changes in the trees’ growth responses caused by the application of fertiliser and the influence of climatic conditions on secondary growth in the trees to which the fertiliser had been applied. As part of the study, the extent of and changes in the growth responses were determined with reference to annual ring widths and earlywood and latewood widths using dendrochronological methods. The research was carried out in four pine stands: two stands of different ages (80 and 110 years) located within the FWTP site and two control stands of corresponding ages located outside that area. Core samples were collected from 12 trees in each stand. We found a two-way impact of potato starch wastewater on secondary growth in the trees under study, with a stimulatory effect (27%–30%) in the first decade of fertiliser application followed in the subsequent years by a strong reduction in growth (30%–45%, depending on the age of the trees). The trends of these changes could be seen in both the overall annual ring widths and the widths of earlywood and latewood. The direction of the changes was the same for trees of different ages, although age was found to have affected the extent and duration of the stimulatory or inhibitory effect. Over the entire period during which the fertiliser was applied, changes occurred in the structure of the wood as manifested in the increased share of earlywood. The sprinkler application of potato starch wastewater and the accompanying irrigation caused a shift in dendroclimatic relationships in comparison to the control plots. Surface irrigation and the resulting changes in water balance reduced the drought susceptibility of the pines under study. At the same time, however, trees weakened by the excessive concentration of toxic nitrates became more sensitive to temperature conditions in winter. The results confirm that the implementation of substances containing significant amounts of organic nitrogen and potassium into forest ecosystems may impair the vigour of trees, reduce stand productivity, cause an imbalance in the ecosystem and may consequently lead to forest degradation.
Genome-Wide Identification and Characterization of Calmodulin and Calmodulin-like Genes Family in Tea Plant and Their Roles under Abiotic Stress
Authors: Kang Rui , Zhao Renliang , Wang Long , Liu Chunhui , Zhang Fen , Zhou Qiongqiong
Published: September 2022

As an important Ca2+ sensor, calmodulin (CaM) and calmodulin-like protein (CML) play core roles in plant growth, development, and response to environmental stimuli. The CaM/CML gene family has been well characterized in various plant species, such as Arabidopsis thaliana, rice, and tomato; however, in the tea plant, the CaM/CML gene family has not been systematically and comprehensively characterized. In the present study, a total of 5 CsCaM and 60 CsCML proteins were identified from the tea plant genome, which were unevenly distributed on the 14 chromosomes of the tea plant. All the proteins contained two to four EF-hand domains. Meanwhile, an integrated analysis of physicochemical properties, sequence structure, motif identification, phylogeny, gene duplication, promoter cis-elements, and RNA-seq expression profiles in the CsCaM/CML gene family was performed. Transcriptome analysis revealed that CsCaM/CMLs were differentially expressed in different tissues of the tea plant, suggesting their potential roles in plant growth and development. The expression profiles associated with various stress treatments revealed that CsCaM/CML genes were involved in a wide range of abiotic factors, including cold and drought stress. Quantitative real-time PCR (qRT-PCR) was also used to validate the differences in expression under abiotic stress. Overall, these findings enhanced our understanding of CsCaM/CML genes and provided useful information for further research into their molecular functions in abiotic stress response, and in multiple physiological processes in the tea plant.
Effects of Two Management Practices on Monthly Litterfall in a Cypress Plantation
Authors: Yang Yulian , Yang Honglin , Wang Qiang , Dong Qing , Yang Jiaping , Wu Lijun , You Chengming , Hu Jinyao , Wu Qinggui
Published: September 2022

Optimizing stand structure can enhance plantation forest ecosystem service functions by regulating litterfall patterns; however, the effects of close-to-nature management on litterfall production remain unclear. Here, we selected three cypress (Cupressus funebris) plantations, including one using the practice of strip filling (SF), one using the practice of ecological thinning (ET), and one pure cypress plantation without any artificial interference. The production of total litterfall and its components (leaf, twig, reproductive organ and miscellaneous litterfall) were investigated monthly over one year from September 2019 to August 2020. Compared with that of the pure plantation, the total annual litterfall production of the SF and ET plantations decreased significantly by 10.8% and 36.44%, respectively. The annual production of leaf and reproductive organ litter was similar to that of total litterfall, but that of twig and miscellaneous litter was higher in the SF and ET plantations than in the pure plantation. Moreover, total, leaf and reproductive organ litterfall production displayed unimodal dynamics regardless of plantation, although the peaks of reproductive organ litter production occurred in different months. In contrast, the production of twig litter showed bimodal dynamics in the pure plantation, while unimodal and irregular dynamics were observed in the plantations with ET and SF, respectively. Additionally, insignificant differences in the isometric growth index of leaf litter and total litterfall were observed. The allometric indices of twig litterfall versus total litterfall, reproductive organ litterfall versus total litterfall, and leaf litterfall versus twig litterfall were higher in the plantations with SF and ET than in the pure plantation. Redundancy analysis (RDA) revealed that diameter at breast height and air temperature were the most important factors shaping the annual and monthly production of litterfall, respectively. These results provide efficient data to support the rectification of the material circulation of cypress plantations and their future management.
Measurement and Spatial Econometric Analysis of Forest Carbon Sequestration Efficiency in Zhejiang Province, China
Authors: Zhu Chenghao , Wang Zhengyi , Ji Biyong , Wang Jianwu , Xu Chang , Xie Binglou
Published: September 2022

Maximizing the carbon sequestration of forested land is important for achieving carbon neutrality. Although some studies have discussed forest carbon sequestration efficiency (FCSE) from the perspective of total factor production, it is being increasingly recognized that forestland use regulates sequestration and emissions. When viewing forestland use as input and carbon emissions as output, there is a lack of empirical evidence on FCSE and its influencing factors. Here, a superefficiency slacks-based measurement model was applied to estimate FCSE for 66 counties in Zhejiang Province, China. The influencing factors and spatial spillover effects of FCSE were also analyzed using a spatial autocorrelation model. The findings showed that over the sample observation period, county FCSE ranged from 0.199 to 1.258, with considerable gaps. The global Moran’s I index showed that county-level FCSE was markedly spatially autocorrelated. Spatially, forestland use, cutting, pests, and diseases had negative spatial spillover effects on FCSE, whereas average annual temperature and precipitation displayed positive spillover effects. These findings suggest that the overall coordination of forest resource supervision and management among counties should be strengthened. The implementation of forestry management models aimed at consolidating or increasing forest carbon sequestration should be emphasized to improve forest quality, thereby promoting FCSE enhancement.
Media Optimization by Response Surface Methodology for the Enhanced Production of Acidic Extracellular Pectinase by the Indigenously Isolated Novel Strain Aspergillus cervinus ARS2 Using Solid-State Fermentation
Authors: Shet Anil R. , Muhsinah Abdullatif Bin , Alsayari Abdulrahman , Achappa Sharanappa , Desai Shivalingsarj V. , Mahnashi Mater H. , Muddapur Uday M. , Shaikh Ibrahim Ahmed , Mannasaheb Basheerahmed Abdulaziz , Khan Aejaz Abdullatif
Published: September 2022

Pectinolytic enzymes are related enzymes that hydrolyze pectic substances. Pectinolytic enzymes are of great interest in industrial applications for softening fruits, extracting and clarifying juices, extracting olive oil, retting textile fibers, preparing gel, and isolating protoplasts. The current work presents acidic extracellular pectinase production using low-cost agro-industrial waste with the indigenously isolated novel strain Aspergillus cervinus. Two fungal isolates, ARS2 and ARS8, with maximum pectinase activity, 41.88 ± 1.57 IU/mL and 39.27 ± 1.14 IU/mL, respectively, were screened out of 27 isolates from decayed fruit peels (orange, banana, and lemon) and soil containing decomposed vegetables. The isolate ARS2, identified as Aspergillus cervinus by molecular characterization, showed the highest pectinase activity of 43.05 ± 1.38IU/mL during screening and was further used for media component screening and optimization studies. To understand their effect on pectinase activity, one-factor-at-a-time (OFAT) studies were conducted on carbon sources, nitrogen sources, and mineral salts. The OFAT results showed the highest pectinase activity for orange peel (carbon source) at 44.51 ± 1.33 IU/mL, peptone (nitrogen source) at 45.05 ± 1.04 IU/mL, and NaH2PO4 (mineral salts) at 43.21 ± 1.12 IU/mL. The most significant media components screened by the Plackett–Burman (PB) design based on the p-value, Pareto chart, and main effect plot, were orange peel (p < 0.001), peptone (p < 0.001), NaH2PO4 (p < 0.001), and KH2PO4 (p < 0.001), which were further optimized using Response Surface Methodology (RSM) and Central Composite Design (CCD). The optimization results for the media components showed a maximum pectinase activity of 105.65 ± 0.31 IU/mL for 10.63 g orange peel, 3.96 g/L peptone, 2.07 g/L KH2PO4, and 2.10 g/L NaH2PO4. Thus, it was discovered that the indigenously isolated novel strain Aspergillus cervinus ARS2 was able to successfully produce a significant amount of pectinase using agro-industrial waste. Therefore, it can be considered for the large-scale optimized production of pectinase to meet industrial demands.
Investigating the Mechanical Performance on Static and Shock Wave Loading of Aramid Fiber-Reinforced Concrete
Authors: Li Yeou-Fong , Wang Hsin-Fu , Syu Jin-Yuan , Ramanathan Gobinathan Kadagathur , Tsai Ying-Kuan
Published: September 2022

Fiber-reinforced concrete (FRC) has been used for over a century to improve the mechanical properties of concrete. Kevlar ® 29 fiber (KF) is one of the most popular aramid fibers used in industrial products. This research investigated the effect of the fiber length, the weight ratio of fiber to cement, the mix-proportion of two fiber lengths, and the sizing on the fiber surface on the mechanical properties of Kevlar fiber-reinforced concrete (KFRC) under static, dynamic, and shock wave loadings. Two lengths of chopped KF and three different weight ratios of fiber to cement were mixed in the KFRC specimens for comparison. Moreover, this study also compared how the five mix-proportions of two fiber lengths affected the mechanical properties of mix-proportion KFRC. KF was dispersed by the pneumatic method first, and then, the separated KF was mixed into the concrete to make KFRC. The results indicated that the KFRC specimens with a 10‰ weight ratio of fiber to cement exhibited the maximum compressive, flexural, and splitting tensile strengths, regardless of whether the fiber length was 12 mm or 24 mm. The main finding showed that the specimen mixed with 24 mm KF could endure the highest impact resistance under different impact energies. From the shock wave test, the external damage on the front and rear faces of all KFRC slabs and the KFRC slab reinforced with two layers of KF sheets was less than that of the benchmark slab. The testing results showed that KF greatly enhanced the static and dynamic mechanical performances of concrete, and the KFRC specimen with a 10‰ weight ratio and 24 mm length KF with sizing exhibited the best performance.
Antibacterial Regularity Mining Beneath the Systematic Activity Database of Lipopeptides Brevilaterins: An Instructive Activity Handbook for Its Food Application
Authors: Liu Yangliu , Han Panpan , Jia Yingmin , Chen Zhou , Li Siting , Ma Aijin
Published: September 2022

Bacterial contamination is a primary threat to food safety. Therefore, the persistent development of natural antibacterial agents has become essential work. The present essay attempts to establish a systematic antibacterial activity database to instruct the food application of brevilaterins, promising antibacterial lipopeptides from Brevibacillus laterosporus S62-9. Minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were systematically collected from 43 species of standard bacteria and 140 strains of isolated bacteria (food spoilage bacteria and antibiotic-resistant bacteria) using a broth dilution method. The results showed that brevilaterins performed a broad-spectrum inhibitory (0.5~128 μg/mL) and bactericidal activity (1~256 μg/mL), especially efficient against Gram-positive bacteria and spoilage bacteria from grain products. Moreover, brevilaterins not only inhibit and kill multiple antibiotic-resistant bacteria but do not readily develop resistance, with a small specific value of MBC/MIC (1~8). Furthermore, brevilaterins would interact with negatively charged sodium dodecyl sulfate and bind amphipathic soybean phospholipid with an affinity constant of KD = 4.70 × 10−4 M. No significant activity difference was found between brevilaterin B and brevilaterin C. Collectively, this work contributed rich antibacterial data of brevilaterins and revealed the antibacterial regularity beneath these data, which can be used as an activity handbook to instruct their application in food safety.
Encapsulation of Functional Plant Oil by Spray Drying: Physicochemical Characterization and Enhanced Anti-Colitis Activity
Authors: Zhang Hao , Xu Zhenxia , Qiao Zhixian , Wang Xu , Tang Hu , Yang Chen , Huang Fenghong
Published: September 2022

In this study, an encapsulation system was developed for functional plant oil delivery. Through a series of orthogonal experiments and single factor experiments, the raw material compositions, emulsification conditions, and spray drying conditions for the preparation of flaxseed oil and safflower seed oil powders were optimized, and the final encapsulation efficiency was as high as 99% with approximately 50% oil loading. The storage stability experiments showed that oil powder’s stability could maintain its physicochemical properties over six months. Oral supplementation of the spray-dried flaxseed oil powder exhibited a significant and better effect than flaxseed oil on alleviating colitis in C57BL/6J mice. It suppressed the pro-inflammatory cell factors, including IL-6 and TNF-α, and repaired gut microbial dysbiosis by increasing the microbial diversity and promoting the proliferation of probiotic taxa such as Allobaculum. This work suggests that spray-dried flaxseed oil powder has great potential as a nutraceutical food, with spray drying being a good alternative technique to improve its bioactivity.
Starfish (Asterias rubens) as a New Source of Marine Lipids: Effect of Season, Size and Oil Extraction Methods
Authors: Sørensen Ann-Dorit Moltke , Getachew Adane Tilahun , Jacobsen Charlotte
Published: September 2022

The increasing demand for oils that contain health-beneficial omega-3 fatty acids calls for new resources or better utilization of existing resources, such as side-streams or underutilized resources to maintain a sustainable fishery. Starfish has been, until recently, an unexploited resource with limited utilization. Currently, starfish is processed into starfish meal for feed. However, the content of bioactive compounds, such as omega-3 fatty acids and phospholipids, could make it a new source of marine oil containing omega-3 fatty acids for human consumption. The aim of this study was to map the composition of bioactive compounds in starfish and starfish meal at different harvesting times to elucidate the content and variation over seasons. The results showed that starfish is a good source of marine omega-3 fatty acids and rich in phospholipids. Some variation was observed in the composition, especially for EPA bound to phospholipids, which was significantly higher in the spring. Traditional extraction using heat and mechanical separation was not applicable to the starfish, and neither was enzyme-assisted extraction. On the other hand, the supercritical CO2 extraction method using EtOH as a co-solvent seemed to be a promising green technology for extracting not only non-polar lipids, but also polar lipids, such as phospholipids. However, the conditions for extraction need further optimization.
Anti-Obesity Effect of Theabrownin from Dark Tea in C57BL/6J Mice Fed a High-Fat Diet by Metabolic Profiles through Gut Microbiota Using Untargeted Metabolomics
Authors: Li Hang-Yu , Huang Si-Yu , Xiong Ruo-Gu , Wu Si-Xia , Zhou Dan-Dan , Saimaiti Adila , Luo Min , Zhu Hui-Lian , Li Hua-Bin
Published: September 2022

The epidemic of obesity is a serious public health problem. In this study, the effect of theabrownin from dark tea on obesity was evaluated by biochemical tests and nuclear magnetic resonance in C57BL/6J mice fed a high-fat diet. A mixture of antibiotics was used to deplete gut microbiota and then fecal microbiota transplant was used to restore gut microbiota. Untargeted metabolomics was used to reveal the effects of theabrownin on metabolic profiles through gut microbiota. The results showed that theabrownin significantly reduced body weight gain (83.0%) and body fat accumulation (30.29%) without affecting appetite. Also, theabrownin promoted lipid clearance with a hepatoprotective effect. The extra antibiotics disrupted the regulation of theabrownin on weight control while fecal microbiota transplant restored the beneficial regulation. That is, gut microbiota was important for theabrownin to reduce body weight gain. The untargeted metabolomics indicated that 18 metabolites were related to the anti-obesity effect of theabrownin mediated by gut microbiota. Furthermore, phenylalanine metabolism, histidine metabolism, as well as protein digestion and absorption pathway played a role in the anti-obesity of theabrownin. Our findings suggested that theabrownin significantly alleviated obesity via gut microbiota-related metabolic pathways, and theabrownin could be used for the prevention and treatment of obesity.
Physicochemical Characteristics and Flavor-Related Compounds of Fresh and Frozen-Thawed Thigh Meats from Chickens
Authors: Barido Farouq Heidar , Kim Hye-Jin , Shin Dong-Jin , Kwon Ji-Seon , Kim Hee-Jin , Kim Dongwook , Choo Hyo-Jun , Nam Ki-Chang , Jo Cheorun , Lee Jun-Heon , Lee Sung-Ki , Jang Aera
Published: September 2022

The physicochemical characteristics and flavor-related compounds of thigh meat derived from diverse Korean native chickens (KNC), namely Hanhyup No. 3 (HH3), Woorimatdag No 1 (WRMD 1), and Woorimatdag No 2 (WRMD 2), under fresh and frozen-thawed conditions were studied and compared with those of commercial broilers (CB). Regardless of the breed, KNC showed a higher (p < 0.05) percentage of linoleic and arachidonic acid. The highest proportion of docosahexaenoic acid was observed in WRMD 2. Despite having a higher collagen content, thigh meat derived from KNC maintained a similar texture profile in comparison to that of CB. The concentrations of most free amino acids (FAA), except for taurine, tryptophan, and carnosine, were higher in frozen-thawed meat than in fresh meat. Regarding volatile organic compounds (VOC), following freezing, the concentration of favorable VOCs increased in CB, but decreased in WRMD 1, suggesting a loss of pleasant flavor in frozen-thawed meat. This study indicated that changes in VOCs, including hydrocarbons (d-limonene, heptadecane, hexadecane, naphthalene, pentadecane, 3-methyl-, tridecane), esters (arsenous acid, tris(trimethylsilyl) ester, decanoic acid, ethyl ester, hexadecanoic acid, ethyl ester), alcohol (1-hexanol, 2-ethyl-), ketones (5,9-undecadien-2-one, 6,10-dimethyl-), and aldehydes (pentadecanal-, tetradecanal, tridecanal), may be a promising marker for distinguishing between fresh and frozen-thawed chicken thigh meat. These findings are of critical importance as preliminary data for developing high-quality chicken meat products.
Dynamic Analysis of a Novel 3D Chaotic System with Hidden and Coexisting Attractors: Offset Boosting, Synchronization, and Circuit Realization
Authors: Dong Chengwei
Published: September 2022

To further understand the dynamical characteristics of chaotic systems with a hidden attractor and coexisting attractors, we investigated the fundamental dynamics of a novel three-dimensional (3D) chaotic system derived by adding a simple constant term to the Yang–Chen system, which includes the bifurcation diagram, Lyapunov exponents spectrum, and basin of attraction, under different parameters. In addition, an offset boosting control method is presented to the state variable, and a numerical simulation of the system is also presented. Furthermore, the unstable cycles embedded in the hidden chaotic attractors are extracted in detail, which shows the effectiveness of the variational method and 1D symbolic dynamics. Finally, the adaptive synchronization of the novel system is successfully designed, and a circuit simulation is implemented to illustrate the flexibility and validity of the numerical results. Theoretical analysis and simulation results indicate that the new system has complex dynamical properties and can be used to facilitate engineering applications.
Process Simulation and Design Considerations for Biodiesel Production from Rubber Seed Oil
Authors: Trirahayu Dhyna Analyes , Abidin Akhmad Zainal , Putra Ridwan P. , Hidayat Achmad Syarif , Safitri Erwina , Perdana Muhammad Iqbal
Published: September 2022

Indonesia is one of the largest rubber producers worldwide. However, rubber seeds still garner less attention due to their low economic value. In fact, the rubber seeds contain 40–50% (w/w) of rubber seed oil (RSO), which is a potential candidate to be used as a feedstock in biodiesel production. In this regard, this study aims to model and simulate the production process of biodiesel from RSO via transesterification reaction, employing methanol and heterogeneous catalyst. The simulation was performed using ASPEN Hysys v11. Acid-based catalyzed esterification was implemented to eliminate soap formation, which may significantly lower biodiesel yield. The results showed that an RSO inlet rate of 1100 L/h with a methanol to oil molar ratio of 1:6 could generate around 1146 L/h biodiesel. Methanol recovery was conducted, an approximately 95% of excess methanol could be regenerated. Simulation results indicated that the properties of the biodiesel produced are compatible with modern diesel engines. Economic analysis also shows that this technology is promising, with excellent investment criteria.
Walnut Oil Oleogels as Milk Fat Replacing System for Commercially Available Chocolate Butter
Authors: Pușcaș Andreea , Tanislav Anda Elena , Mureșan Andruţa Elena , Fărcaș Anca Corina , Mureșan Vlad
Published: September 2022

A breakfast spread named chocolate butter exists on the market. For economic and technological reasons, cream in the original recipe is replaced with vegetable oils such as palm oil or by partially hydrogenated sunflower oil. The study aims to reformulate chocolate flavor butter, using cold pressed walnut oil (WO) oleogels (OGs) structured with 10% waxes and monoglyceride (MG), as a milk fat replacing system. The rheological, textural and microscopic characteristics of the oleogels and the spreads were compared. Oil binding capacity (OBC) and colorimetry were also assessed. Fourier transform infrared studies were used to monitor the composition of the samples. Oleogels and oleogel based chocolate butter behaved like strong gels (G’ > G”). The use of candelilla wax (CW) led to the formation of a much firmer spread (S-CW), with a hardness of 3521 g and G’LVR of 139,920 Pa, while the monoglyceride-based spread (S-MG) registered a hardness of 1136 g and G’LVR 89,952 Pa. In the spreadability test, S-CW registered a hardness of 3376 g and hardness work of 113 mJ, comparable to the commercially available chocolate butter. The formulated spreads exhibited shear thinning effects, and increased viscosity with decreasing temperature. A large round peak at 3340 cm−1 was present in the spectra of the candelilla wax-based oleogel (OG-CW) and the reference spreads due to hydrogen bonding, but was absent in S-CW or S-MG. The FTIR spectra of the alternative spreads exhibited the same peaks as the WO and the oleogels, but with differences in the intensities. S-CW exhibited a dense crystal network, with spherulitic crystals of 0.66–1.73 µm, which were statistically similar to those of the reference made from cream (S-cream). S-MG exhibited the lowest stability upon centrifugation, with an OBC of 99.76%. Overall, both oleogel-based chocolate spreads can mimic the properties of the commercially existing chocolate butter references.
Hydrotropic Hydrogels Prepared from Polyglycerol Dendrimers: Enhanced Solubilization and Release of Paclitaxel
Authors: Ooya Tooru , Lee Jaehwi
Published: September 2022

Polyglycerol dendrimers (PGD) exhibit unique properties such as drug delivery, drug solubilization, bioimaging, and diagnostics. In this study, PGD hydrogels were prepared and evaluated as devices for controlled drug release with good solubilization properties. The PGD hydrogels were prepared by crosslinking using ethylene glycol diglycidylether (EGDGE). The concentrations of EGDGE and PGDs were varied. The hydrogels were swellable in ethanol for loading paclitaxel (PTX). The amount of PTX in the hydrogels increased with the swelling ratio, which is proportional to EGDGE/OH ratio, meaning that heterogeneous crosslinking of PGD made high dense region of PGD molecules in the matrix. The hydrogels remained transparent after loading PTX and standing in water for one day, indicating that PTX was dispersed in the hydrogels without any crystallization in water. The results of FTIR imaging of the PTX-loaded PGD hydrogels revealed good dispersion of PTX in the hydrogel matrix. Sixty percent of the loaded PTX was released in a sink condition within 90 min, suggesting that the solubilized PTX would be useful for controlled release without any precipitation. Polyglycerol dendrimer hydrogels are expected to be applicable for rapid release of poorly water-soluble drugs, e.g., for oral administration.
Structurally Stable, High-Strength Graphene Oxide/Carbon Nanotube/Epoxy Resin Aerogels as Three-Dimensional Skeletal Precursors for Wave-Absorbing Materials
Authors: Zhang Lina , Song Guojun , Zhao Zetian , Ma Lichun , Xu Hui , Wu Guanglei , Song Yinghu , Liu Yinuo , Qiu Lihan , Li Xiaoru
Published: September 2022

Three-dimensional (3D) graphene oxide aerogel (GOA) is one of the best fillers for composites for microwave absorption. However, its further development has been hindered by the poor mechanical properties. Methodology to improve the mechanical properties of the aerogel remains an urgent challenge. Herein, graphene oxide/carbon nanotube/epoxy resin composite aerogel (GCEA) was successfully prepared by a facile method. The results showed that the prepared GCEA with the hierarchical and 3D cross-linked structures exhibited excellent compression performance, structural and thermal stability, high hydrophilicity, and microwave absorption. The prepared GCEA recovered from multiple large strain cycles without significant permanent deformation. The minimum reflection loss (RL) was −39.60 dB and the maximum effective absorption bandwidth (EAB) was 2.48 GHz. The development of the enhanced GO aerogels will offer a new approach to the preparation of 3D microwave-absorbing skeletal materials with good mechanical properties.
Co-Treatment of Erythroid Cells from β-Thalassemia Patients with CRISPR-Cas9-Based β039-Globin Gene Editing and Induction of Fetal Hemoglobin
Authors: Cosenza Lucia Carmela , Zuccato Cristina , Zurlo Matteo , Gambari Roberto , Finotti Alessia
Published: September 2022

Gene editing (GE) is an efficient strategy for correcting genetic mutations in monogenic hereditary diseases, including β-thalassemia. We have elsewhere reported that CRISPR-Cas9-based gene editing can be employed for the efficient correction of the β039-thalassemia mutation. On the other hand, robust evidence demonstrates that the increased production of fetal hemoglobin (HbF) can be beneficial for patients with β-thalassemia. The aim of our study was to verify whether the de novo production of adult hemoglobin (HbA) using CRISPR-Cas9 gene editing can be combined with HbF induction protocols. The gene editing of the β039-globin mutation was obtained using a CRISPR-Cas9-based experimental strategy; the correction of the gene sequence and the transcription of the corrected gene were analyzed by allele-specific droplet digital PCR and RT-qPCR, respectively; the relative content of HbA and HbF was studied by high-performance liquid chromatography (HPLC) and Western blotting. For HbF induction, the repurposed drug rapamycin was used. The data obtained conclusively demonstrate that the maximal production of HbA and HbF is obtained in GE-corrected, rapamycin-induced erythroid progenitors isolated from β039-thalassemia patients. In conclusion, GE and HbF induction might be used in combination in order to achieve the de novo production of HbA together with an increase in induced HbF.
Impact of Methionine Synthase Reductase Polymorphisms in Chronic Myeloid Leukemia Patients
Authors: Elderdery Abozer Y. , Tebein Entesar M. , Alenazy Fawaz O. , Elkhalifa Ahmed M. E. , Shalabi Manar G. , Abbas Anass M. , Alhassan Hassan H. , Davuljigari Chand B. , Mills Jeremy
Published: September 2022

Introduction： Metabolism methionine and of folate play a vital function in cellular methylation reactions, DNA synthesis and epigenetic process.However, polymorphisms of methionine have received much attention in recent medical genetics research. Objectives: To ascertain whether the common polymorphisms of the MTRR (Methionine Synthase Reductase) A66G gene could play a role in affecting susceptibility to Chronic Myeloid Leukemia (CML) in Sudanese individuals. Methods: In a case-controlled study, we extracted and analyzed DNA from 200 CML patients and 100 healthy control subjects by the PCR-RFLP method. Results: We found no significant difference in age orgender between the patient group and controls. The MTRR A66G genotypes were distributed based on the Hardy-Weinberg equilibrium (p > 0.05). The variation of MTRR A66G was less significantly frequent in cases with CML (68.35%) than in controls (87%) (OR = 0.146, 95% CI = 0.162–0.662, p < 0.002). Additionally, AG and GG genotypes and G allele were reducing the CML risk (Odds ratio [OR] = 0.365; 95% CI [0.179–0.746]; p = 0.006; OR = 0.292; 95% CI [0.145–0.590]; p = 0.001 and OR = 0.146; 95% CI [0.162–0.662]; p = 0.002 and OR = 2.0; 95% CI [1.3853–2.817]; respectively, (p = 0.000)). Conclusions: Our data demonstrated that heterozygous and homozygous mutant genotypes of MTRR polymorphisms were associated with decreased risk of developing CML in the Sudanese population.
Transcriptome Analysis Revealed Hormone Pathways and bZIP Genes Responsive to Decapitation in Sunflower
Authors: Dong Lili , Wu Yu , Zhang Jianbin , Deng Xinyi , Wang Tian
Published: September 2022

Decapitation is an essential agricultural practice and is a typical method for analyzing shoot branching. However, it is unclear exactly how decapitation controls branching. In this study, the decapitation of sunflower plants led to the development of lateral buds, accompanied by a decrease in indole-3-acetic acid (IAA) and abscisic acid (ABA) levels and an increase in cytokinin (CK) levels. Additionally, 82 members of the HabZIP family were discovered and categorized into 9 groups, using phylogenetic and conservative domain analysis. The intron/exon structure and motif compositions of HabZIP members were also investigated. Based on tissue-specific expression and expression analysis following decapitation derived from the transcriptome, several HabZIP members may be involved in controlling decapitation-induced bud outgrowth. Therefore, it is hypothesized that the dynamic variations in hormone levels, in conjunction with particular HabZIP genes, led to the development of axillary buds in sunflowers following decapitation.
Development of Genomic Resources in Mexican Bursera (Section: Bullockia: Burseraceae): Genome Assembly, Annotation, and Marker Discovery for Three Copal Species
Authors: Rico Yessica , Lorenzana Gustavo P. , Benítez-Pineda Carlos A. , Olukolu Bode A.
Published: September 2022

Bursera comprises ~100 tropical shrub and tree species, with the center of the species diversification in Mexico. The genomic resources developed for the genus are scarce, and this has limited the study of the gene flow, local adaptation, and hybridization dynamics. In this study, based on ~155 million Illumina paired-end reads per species, we performed a de novo genome assembly and annotation of three Bursera species of the Bullockia section: Bursera bipinnata, Bursera cuneata, and Bursera palmeri. The total lengths of the genome assemblies were 253, 237, and 229 Mb for B. cuneata, B. palmeri, and B. bipinnata, respectively. The assembly of B. palmeri retrieved the most complete and single-copy BUSCOs (87.3%) relative to B. cuneata (86.5%) and B. bipinnata (76.6%). The ab initio gene prediction recognized between 21,000 and 32,000 protein-coding genes. Other genomic features, such as simple sequence repeats (SSRs), were also detected. Using the de novo genome assemblies as a reference, we identified single-nucleotide polymorphisms (SNPs) for a set of 43 Bursera individuals. Moreover, we mapped the filtered reads of each Bursera species against the chloroplast genomes of five Burseraceae species, obtaining consensus sequences ranging from 156 to 160 kb in length. Our work contributes to the generation of genomic resources for an important but understudied genus of tropical-dry-forest species.
Mapping Retrotransposon LINE-1 Sequences into Two Cebidae Species and Homo sapiens Genomes and a Short Review on Primates
Authors: Milioto Vanessa , Perelman Polina L. , Paglia Laura La , Biltueva Larisa , Roelke Melody , Dumas Francesca
Published: September 2022

This work focuses on the distribution of LINE-1 (a Long Interspersed Nuclear Element) in primates and its role during evolution and as a constituent of the architecture of primate genomes. To pinpoint the LINE-1 repeat distribution and its role among primates, LINE-1 probes were mapped onto chromosomes of Homo sapiens (Hominidae, Catarrhini), Sapajus apella, and Cebus capucinus (Cebidae, Platyrrhini) using fluorescence in situ hybridisation (FISH). The choice of platyrrhine species are due to the fact they are taxa characterised by a high level of rearrangements; for this reason, they could be a useful model for the study of LINE-1 and chromosome evolution. LINE-1 accumulation was found in the two Cebidae at the centromere of almost all acrocentric chromosomes 16–22 and on some bi-armed chromosomes. LINE-1 pattern was similar in the two species but only for chromosomes 6, 8, 10, and 18, due to intrachromosomal rearrangements in agreement with what was previously hypothesised as through g banding. LINE-1 interstitial accumulation was found in humans on the 1, 8, 9, 13–15, and X chromosomes; on chromosomes 8, 9, and 13–15, the signal was also at the centromeric position. This is in agreement with recent and complete molecular sequence analysis of human chromosomes 8 and some acrocentric ones. Thus, the hypothesis regarding a link between LINE-1 and centromeres as well as a link with rearrangements are discussed. Indeed, data analysis leads us to support a link between LINE-1 and inter- and intrachromosomal rearrangements, as well as a link between LINE-1 and structural functions at centromeres in primates.
Classification of Protein Sequences by a Novel Alignment-Free Method on Bacterial and Virus Families
Authors: Guan Mengcen , Zhao Leqi , Yau Stephen S.-T.
Published: September 2022

The classification of protein sequences provides valuable insights into bioinformatics. Most existing methods are based on sequence alignment algorithms, which become time-consuming as the size of the database increases. Therefore, there is a need to develop an improved method for effectively classifying protein sequences. In this paper, we propose a novel accumulated natural vector method to cluster protein sequences at a lower time cost without reducing accuracy. Our method projects each protein sequence as a point in a 250-dimensional space according to its amino acid distribution. Thus, the biological distance between any two proteins can be easily measured by the Euclidean distance between the corresponding points in the 250-dimensional space. The convex hull analysis and classification perform robustly on virus and bacteria datasets, effectively verifying our method.
Rare Heterozygous PCSK1 Variants in Human Obesity: The Contribution of the p.Y181H Variant and a Literature Review
Authors: Van Dijck Evelien , Beckers Sigri , Diels Sara , Huybrechts Tammy , Verrijken An , Van Hoorenbeeck Kim , Verhulst Stijn , Massa Guy , Van Gaal Luc , Van Hul Wim
Published: September 2022

Recently, it was reported that heterozygous PCSK1 variants, causing partial PC1/3 deficiency, result in a significant increased risk for obesity. This effect was almost exclusively generated by the rare p.Y181H (rs145592525, GRCh38.p13 NM_000439.5:c.541T>C) variant, which affects PC1/3 maturation but not enzymatic capacity. As most of the identified individuals with the heterozygous p.Y181H variant were of Belgian origin, we performed a follow-up study in a population of 481 children and adolescents with obesity, and 486 lean individuals. We identified three obese (0.62%) and four lean (0.82%) p.Y181H carriers (p = 0.506) through sanger sequencing and high resulting melting curve analysis, indicating no association with obesity. Haplotype analysis was performed in 13 p.Y181H carriers, 20 non-carriers (10 with obesity and 10 lean), and two p.Y181H families, and showed identical haplotypes for all heterozygous carriers (p < 0.001). Likewise, state-of-the-art literature concerning the role of rare heterozygous PCSK1 variants implies them to be rarely associated with monogenic obesity, as first-degree carrier relatives of patients with PC1/3 deficiency are mostly not reported to be obese. Furthermore, recent meta-analyses have only indicated a robust association for scarce disruptive heterozygous PCSK1 variants with obesity, while clinical significance is less or sometimes lacking for most nonsynonymous variants.
Developing Mobile Applications with Augmented Reality and 3D Photogrammetry for Visualisation of Cold-Water Coral Reefs and Deep-Water Habitats
Authors: de Oliveira Larissa Macedo Cruz , Oliveira Priscila Almeida de , Lim Aaron , Wheeler Andrew J. , Conti Luis Americo
Published: September 2022

Cold-water coral (CWC) reefs are considered “hotspots” of biodiversity in deep-sea environments. Like tropical coral reefs, these habitats are subject to climate and anthropogenic threats. The use of remotely operated vehicles (ROVSs) in combination with three-dimensional (3D) modelling and augmented reality (AR) has enabled detailed visualisation of terrestrial and marine environments while promoting data accessibility and scientific outreach. However, remote environments such as CWC reefs still present challenges with data acquisition, which impacts the further understanding of these environments. This study aims to develop a mobile application using structure-from-motion (SfM) 3D photogrammetric data and AR for the visualisation of CWC reefs. The mobile application was developed to display 3D models of CWC reefs from the Piddington Mound area, southwest of Ireland. The 3D models were tested at different resolutions to analyse the visualisation experience and trade-off between resolution and application size. The results from the 3D reconstructions with higher resolution indicate that the combination of SfM, AR, and mobile phones is a promising tool for raising awareness and literacy regarding CWC and deep-water habitats. This study is the first of its kind to showcase CWC habitats accessible to anyone, anywhere with a mobile phone and internet connectivity.
Pre-Orogenic Tectonostratigraphic Evolution of the European Distal Margin-Alpine Tethys Transition Zone in High-Pressure Units of the Southwestern Alps
Authors: Balestro Gianni , Festa Andrea , Cadoppi Paola , Groppo Chiara , Roà Matthieu
Published: September 2022

Geological mapping, stratigraphic observations, and U/Pb dating allow reconstructing the pre-orogenic setting of the transition zone between the distal European passive margin and the Alpine Tethys in the southwestern Alps. Although convergent tectonics overprinted the syn-rift Jurassic tectonic features, our data document an articulated Jurassic physiography. From the distal European passive margin oceanward, we distinguished: the Dronero Unit (the southernmost Dora Maira massif), represents a continental margin composite basement wherein monometamorphic metasediments are interlayered with Late Permian (253.8 ± 2.7 Ma) metavolcanic rocks; the Sampeyre Unit, represents a structural high consisting of Lower Triassic Verrucano-facies siliciclastic metasediments unconformably sealed by Cretaceous calcschist bearing Globotruncana sp.; the Maira Unit, corresponds to a Middle Triassic platform succession detached from the Sampeyre Unit; the Grana Unit, corresponds to a Late Triassic–Late Jurassic platform to basin succession; the Queyras Schistes Lustrès Complex, represents the ocean basin succession. Tectonic slices of Cambrian (513.9 ± 2.7 Ma) metadiorite hosted in the Valmala Shear Zone, separating the Dronero Unit from the underlying (U)HP units of the Dora Maira massif, suggests a potential pre-Alpine activation of the shear zone.
Tove Ditlevsen’s Witness of Trauma as a Source of Hope
Authors: Allen Julie K.
Published: September 2022

The defining life experience of the Danish writer Tove Ditlevsen (1917–1976) was domestic trauma, both externally- and self-inflicted. Born at the end of the first World War amid an economic depression, Ditlevsen grew up in a hardscrabble working-class neighborhood of Copenhagen, lived through the Nazi occupation of her country during World War II, cycled through unhealthy sexual relationships, underwent illegal abortions and unnecessary surgeries, suffered from depression and prescription drug addiction, and died by suicide at the age of fifty-eight. Instead of repressing or denying her traumatic experiences, however, Ditlevsen chose to confront, reinscribe, and transform them in her literary texts, finding and offering hope that exposing secrets to public scrutiny can lead to acceptance and healing. In her searingly candid poetry, fiction, essays, and memoirs, Ditlevsen exemplifies the efficacy of working through trauma: she confronts the fraught choices and abusive relationships by which her life was shaped candidly and unapologetically as an act of survival. In the process of bearing witness on the printed page to domestic trauma and its consequences, Ditlevsen models the vital role of literature, for both readers and writers, in documenting, processing, and overcoming traumatic experiences.
Time-Series Analysis and Healthcare Implications of COVID-19 Pandemic in Saudi Arabia
Authors: Zrieq Rafat , Kamel Souad , Boubaker Sahbi , Algahtani Fahad D. , Alzain Mohamed Ali , Alshammari Fares , Alshammari Fahad Saud , Aldhmadi Badr Khalaf , Atique Suleman , Al-Najjar Mohammad A. A. , Villareal Sandro C.
Published: September 2022

The first case of coronavirus disease 2019 (COVID-19) in Saudi Arabia was reported on 2 March 2020. Since then, it has progressed rapidly and the number of cases has grown exponentially, reaching 788,294 cases on 22 June 2022. Accurately analyzing and predicting the spread of new COVID-19 cases is critical to develop a framework for universal pandemic preparedness as well as mitigating the disease’s spread. To this end, the main aim of this paper is first to analyze the historical data of the disease gathered from 2 March 2020 to 20 June 2022 and second to use the collected data for forecasting the trajectory of COVID-19 in order to construct robust and accurate models. To the best of our knowledge, this study is the first that analyzes the outbreak of COVID-19 in Saudi Arabia for a long period (more than two years). To achieve this study aim, two techniques from the data analytics field, namely the auto-regressive integrated moving average (ARIMA) statistical technique and Prophet Facebook machine learning technique were investigated for predicting daily new infections, recoveries and deaths. Based on forecasting performance metrics, both models were found to be accurate and robust in forecasting the time series of COVID-19 in Saudi Arabia for the considered period (the coefficient of determination for example was in all cases more than 0.96) with a small superiority of the ARIMA model in terms of the forecasting ability and of Prophet in terms of simplicity and a few hyper-parameters. The findings of this study have yielded a realistic picture of the disease direction and provide useful insights for decision makers so as to be prepared for the future evolution of the pandemic. In addition, the results of this study have shown positive healthcare implications of the Saudi experience in fighting the disease and the relative efficiency of the taken measures.