bluuebunny/crossref_metadata_2025 huggingface dataset:
Prioritising topics for the undergraduate ENT curriculum
Authors: Constable J D , Moghul G A , Leighton P , Schofield S J , Daniel M
Published: May 2017

Objective:

Knowledge of ENT is important for many doctors, but undergraduate time is limited. This study aimed to identify what is thought about ENT knowledge amongst non-ENT doctors, and the key topics that the curriculum should focus on.

Methods:

Doctors were interviewed about their views of ENT knowledge amongst non-ENT doctors, and asked to identify key topics. These topics were then used to devise a questionnaire, which was distributed to multiple stakeholders in order to identify the key topics.

Results:

ENT knowledge was generally thought to be poor amongst doctors, and it was recommended that undergraduate ENT topics be kept simple. The highest rated topics were: clinical examination; when to refer; acute otitis media; common emergencies; tonsillitis and quinsy; management of ENT problems by non-ENT doctors; stridor and stertor; otitis externa; and otitis media with effusion.

Conclusion:

This study identified a number of key ENT topics, and will help to inform future development of ENT curricula.
Reproduction and population structure ofOctopus mimusfished in a Marine Protected Area of Ecuador
Authors: Markaida Unai , Flores Luis , Arias Evelyn , Mora Elba
Published: May 2017

Octopus mimusis the main octopus targeted by the small-scale fishery in the Eastern Pacific. Commercial catches of octopus from the Reserva de Producción Faunística Marino-Costera Puntilla de Santa Elena (REMACOPSE) Marine Protected Area in Ecuador were sampled from July to December 2013 in order to describe the reproduction and population structure of this octopus. The 4171 sampled octopuses ranged from 56 to 250 mm in mantle length (ML) and 45 to 3178 g in body weight (BW). Mean octopus weight did not change monthly. Males were mostly mature while the majority of females were immature. Presence of some mature and a few spent females in all months sampled suggests that the population shows an extended spawning period. No spawning peak could be properly defined. Sex ratios did not significantly shift from the expected 1:1 in most samples. Females mature at a larger size (1234 g, BW; 165 mm, ML) than males (487 g BW; 114 mm ML). We recommend considering a minimum legal size of 1200 g BW. A year-long sampling programme forO. mimusshould also be completed in order to detect any spawning peak.
Nerolidol, the main constituent of Piper aduncum essential oil, has anti-Leishmania braziliensis activity
Authors: CEOLE LIGIA FERNANDA , CARDOSO MARIA DAS GRAÇAS , SOARES MAURILIO JOSÉ
Published: May 2017

Leishmania (Viannia) braziliensis is a protozoan that causes mucocutaneous leishmaniasis, which is an infectious disease that affects more than 12 million people worldwide. The available treatment is limited, has side-effects or is inefficient. In a search for alternative compounds of natural origin, we tested the microbicidal activity of Piper aduncum essential oil (PaEO) on this parasite. Our data showed that PaEO had an inhibitory effect on the growth of L. braziliensis promastigotes with an IC50/24 h=77·9 µg mL−1. The main constituent (nerolidol: 25·22%) presented a similar inhibitory effect (IC50/24 h = 74·3 µg mL−1). Ultrastructural observation of nerolidol-treated parasites by scanning and transmission electron microscopies revealed cell shrinkage and morphological alterations in the mitochondrion, nuclear chromatin and flagellar pocket. Flow cytometry analysis showed a reduction in the cell size, loss of mitochondrial membrane potential, phosphatidylserine exposure and DNA degradation, which when associated with the morphological changes indicated that nerolidol induced incidental cell death in the L. braziliensis promastigotes. The results presented here indicate that nerolidol derivatives are promising compounds for further evaluation against Leishmania parasites.
Neurocognitive deficits in schizophrenia. Are we making mountains out of molehills?
Authors: Moritz S. , Klein J. P. , Desler T. , Lill H. , Gallinat J. , Schneider B. C.
Published: May 2017

Background

Most original studies and all meta-analyses conducted to date converge on the conclusion that patients with schizophrenia display rather generalized neurocognitive deficits. For the present study, we reopen this seemingly closed chapter and examine whether important influences, such as lack of motivation and negative attitudes towards cognitive assessment, result in poorer secondary neuropsychological performance.

Method

A sample of 50 patients with an established diagnosis of schizophrenia were tested for routine neurocognitive assessment and compared to 60 nonclinical volunteers. Before and after the assessment, subjective momentary influences were examined (e.g. motivation, concerns about assessment, fear about poor outcome) for their impact on performance using a new questionnaire called theMomentary Influences, Attitudes and Motivation Impact(MIAMI)on Cognitive Performance Scale.

Results

As expected, patients performed significantly worse than controls on all neurocognitive domains tested (large effect size, on average). However, patients also displayed more subjective momentary impairment, as well as more fears about the outcome and less motivation than controls. Mediation analyses indicated that these influences contributed to (secondary) poorer neurocognitive performance. Differences in neurocognitive scores shrank to a medium effect size, on average, when MIAMI scores were accounted for.

Conclusions

The data argue that performance on measures of neurocognition in schizophrenia are to a considerable extent due to secondary factors. Poor motivation, fears and momentary impairments distinguished patients from controls and these variables heavily impacted performance. Before concluding that neurocognitive deficits in psychiatric patients are present, clinicians should take these confounding influences into account. Although patients with schizophrenia achieved, on average, worse test scores than controls, a large subgroup displayed spared performance.
Distinct phasic and sustained brain responses and connectivity of amygdala and bed nucleus of the stria terminalis during threat anticipation in panic disorder
Authors: Brinkmann L. , Buff C. , Feldker K. , Tupak S. V. , Becker M. P. I. , Herrmann M. J. , Straube T.
Published: May 2017

Background

Panic disorder (PD) patients are constantly concerned about future panic attacks and exhibit general hypersensitivity to unpredictable threat. We aimed to reveal phasic and sustained brain responses and functional connectivity of the amygdala and the bed nucleus of the stria terminalis (BNST) during threat anticipation in PD.

Methods

Using functional magnetic resonance imaging (fMRI), we investigated 17 PD patients and 19 healthy controls (HC) during anticipation of temporally unpredictable aversive and neutral sounds. We used a phasic and sustained analysis model to disentangle temporally dissociable brain activations.

Results

PD patients compared with HC showed phasic amygdala and sustained BNST responses during anticipation of aversive v. neutral stimuli. Furthermore, increased phasic activation was observed in anterior cingulate cortex (ACC), insula and prefrontal cortex (PFC). Insula and PFC also showed sustained activation. Functional connectivity analyses revealed partly distinct phasic and sustained networks.

Conclusions

We demonstrate a role for the BNST during unpredictable threat anticipation in PD and provide first evidence for dissociation between phasic amygdala and sustained BNST activation and their functional connectivity. In line with a hypersensitivity to uncertainty in PD, our results suggest time-dependent involvement of brain regions related to fear and anxiety.
Internal Reflecting Horizons in Spitsbergen Glaciers
Authors: Bamber J. L.
Published: May 2017

A single pronounced internal reflecting horizon has been observed on radio echo-sounding from over 30 glaciers in Spitsbergen. They are often present along the entire length of the glacier, remaining at a fairly constant depth (100–200 m) below the ice surface. Echo-strength data from radio echo-sounding have been used to obtain reflection coefficients, for these horizons, of between -15 and -25 dB. Combined with results of ice-core studies, the possible causes of this internal layer are investigated. The presence of water is found to be the most likely explanation, indicating the existence, at depth, of a layer of temperate ice.
Stagnant Ice at the Bed of White Glacier, Axel Heiberg Island. N.W.T., Canada
Authors: Blatter Heinz
Published: May 2017

A total of 400 soundings along 15 profiles were obtained on White Glacier, Axel Heiberg Island, N.W.T. with a monopulse radar equipment that was rebuilt according to a model of the US Geological Survey. The resulting data allowed maps to be compiled of the ice thickness for the glacier tongue, The radio echo-sounding data and englacial temperature measurements give some indication of the existence of stagnant ice in depressions of the glacier bed in the accumulation zone of White Glacier.
New Environmental Line Feature-based Vision Navigation: Design and Analysis
Authors: Li Zeyu , Wang Jinling , Chen Kai , Sun Yu
Published: May 2017

Vision navigation using environmental features has been widely applied when satellite signals are not available. However, the matching performance of traditional environmental features such as keypoints degrades significantly in weakly textured areas, deteriorating navigation performance. Further, the user needs to evaluate and assure feature matching quality. In this paper, a new feature, named Line Segment Intersection Feature (LSIF), is proposed to solve the availability problem in weakly textured regions. Then a combined descriptor involving global structure and local gradient is designed for similarity comparison. To achieve reliable point-to-point matching, a coarse-to-fine matching algorithm is developed, which improves the performance of the point set matching algorithm. Finally, a framework of matching quality evaluation is proposed to assure matching performance. Through the comparison, it is demonstrated that the proposed new feature has superior overall performance especially on correctly matched numbers of keypoints and matching correctness. Also, using real image sets with weak texture, it is shown that the proposed LSIF can achieve improved navigation solutions with high continuity and accuracy.
Perceived Support and the Retirement Expectations of Sexual Minority Adults
Authors: Mock Steven E. , Schryer Emily
Published: May 2017

Malgré l’importance de la planification de la retraite chez une population vieillissante, on sait peu sur la planification de la retraite par les adultes appartenant à la minorité sexuelle (homosexuels, lesbiennes, bisexuels) et leurs défis potentiellement uniques. Nous avons comparé les perceptions concernant la planification de la retraite de ces adultes et celle des adultes hétérosexuels, en examinant le rôle potentiel du soutien social. Il n’y avait pas de différences significatives entre les minorités sexuelles et les adultes hétérosexuels concernant l’âge prévu à la retraite, l’âge réel de la retraite et l’adéquation prévue du revenu pendant la retraite; les niveaux plus élevés de soutien perçus et une plus grande certitude dans la planification étaient associés à un âge plus précoce à la retraite. Le soutien perçu a également montré une association plus forte avec la planification de la retraite chez les adultes appartenant à la minorité sexuelle, comparativement aux hétérosexuels, de sorte qu’un faible soutien perçu était associé à la retraite à un âge plus avancé et des niveaux inférieurs de soutien perçu étaient liés à une diminution de l’adéquation du revenu à la retraite pour les adultes de la minorité sexuelle.
The calculus of dependent lambda eliminations
Authors: STUMP AARON
Published: May 2017

Modern constructive type theory is based on pure dependently typed lambda calculus, augmented with user-defined datatypes. This paper presents an alternative called the Calculus of Dependent Lambda Eliminations, based on pure lambda encodings with no auxiliary datatype system. New typing constructs are defined that enable induction, as well as large eliminations with lambda encodings. These constructs are constructor-constrained recursive types, and a lifting operation to lift simply typed terms to the type level. Using a lattice-theoretic denotational semantics for types, the language is proved logically consistent. The power of CDLE is demonstrated through several examples, which have been checked with a prototype implementation called Cedille.
Exceptional flooding tolerance in the totipotent recalcitrant seeds ofEugenia stipitata
Authors: Calvi Geângelo P. , Anjos Antônio M. G. , Kranner Ilse , Pritchard Hugh W. , Ferraz Isolde D. K.
Published: May 2017

Eugenia stipitataoccurs along rivers in Western Amazonia and produces berry-type fruits with economic potential. Its large recalcitrant (i.e. desiccation-intolerant) seeds have been proposed as a model to study seed stress response, as no apparent differentiation between the embryonic axis and the fused cotyledons are visible. Here, the longevity of submerged seeds was analysed with a view to understanding adaptive mechanisms to seasonal flooding. Submerged seeds began germinating after 2 months. After 1 year, 87 and 96% total germination was reached when seeds were submerged under a water column of 6 cm (where seedlings could emerge from under the water) and 26 cm (where seedlings could not reach the water surface), respectively. Seedling morphology was altered underwater, with short internodes and rudimentary leaf blades, and when submersion was terminated, seedlings transplanted to nursery conditions recovered a normal phenotype. Furthermore, when seedlings were detached from the seeds, the ‘resown’ seeds produced a second, normal seedling within 9 months. Concentrations of the antioxidant glutathione, which was measured as a stress marker, increased with submersion time in water. Seeds that had developed roots and shoots underwater had higher concentrations of glutathione disulphide than non-germinated seeds, suggesting that the flooding stress was more intense for seedlings than seeds, although more oxidizing cellular redox environments are also consistent with the conditions required for differentiation. Submergence underwater is recommended for storage of the recalcitrant seeds ofE. stipitatafor up to 1 year.
Gender and Politics in Interwar and Vichy France
Authors: CAMPBELL CAROLINE
Published: May 2017

One of the defining paradoxes of interwar France was the coexistence of a deep-rooted belief in national decadence with the development of a wide range of innovative organisations, cumulatively mobilising millions of people, as a means of fighting this supposed decline. While women played a key role in perpetuating the belief that the Republic was deteriorating, created numerous politically-oriented groups and entered into the government as ministers for the first time, these facts have barely entered into scholarly analysis of the state of France's political culture. Beginning in the 1960s a narrative of stagnation tended to dominate scholars’ interpretations of the interwar years. Reflective of the times, gender was absent from such analyses, as scholars defined ‘politics’ in certain ways and assumed that political actors were men. The influential political scientist Stanley Hoffman, for example, insisted that this was a period of stalemate, essentially the consequence of a failure to modernise during the Third Republic (1870–1940). Hoffman argued that peasants, small business and the bourgeoisie coalesced to advocate for protectionist measures and resist social and economic reforms. This conservative agenda was facilitated by governments that sought to limit economic change, which contributed to ministerial instability: during the interwar period, the French government changed forty-seven times, compared to thirty in Poland and Romania, nine in Great Britain and an average of one per year in Weimar Germany, Belgium and Sweden. For Anglophone and Francophone proponents of the idea of a systemic crisis, the Third Republic appears fundamentally flawed, crippled by an intrinsic defect rather than a democratic government that opened spaces for dynamic groups and movements to effect real change.
Land ownership, tax farming and the social structure of local credit markets in the Ottoman Balkans, 1685–1855
Authors: Kokdas Irfan
Published: May 2017

This article studies how the emergence of new political elites and changes in land tenure relationships shaped the socio-economic profile of local credit markets in the Ottoman Balkans between the late seventeenth and early nineteenth centuries. By using probate inventories and court records for the cities of Salonika (including Karaferye), Vidin and Ruse, I compare how the expansion of tax-farming institutions and the concentration of land ownership influenced the social characteristics of lending activities. I find that, in spite of institutional and political similarities, the evolution of local credit markets did not follow a homogeneous pattern. Contrary to the consensus view in the existing literature, local political and military elites, which most tax farmers and large landowners belonged to, did not play a dominant role as moneylenders. Civilians (such as merchants and artisans) together with other social groups, including janissaries and religious functionaries, provided the bulk of informal credit to local communities (including elites) in the three urban areas.
A scalable architecture for data-intensive natural language processing
Authors: BELOKI ZUHAITZ , ARTOLA XABIER , SOROA AITOR
Published: May 2017

Computational power needs have greatly increased during the last years, and this is also the case in the Natural Language Processing (NLP) area, where thousands of documents must be processed, i.e., linguistically analyzed, in a reasonable time frame. These computing needs have implied a radical change in the computing architectures and big-scale text processing techniques used in NLP. In this paper, we present a scalable architecture for distributed language processing. The architecture uses Storm to combine diverse NLP modules into a processing chain, which carries out the linguistic analysis of documents. Scalability requires designing solutions that are able to run distributed programs in parallel and across large machine clusters. Using the architecture presented here, it is possible to integrate a set of third-party NLP modules into a unique processing chain which can be deployed onto a distributed environment, i.e., a cluster of machines, so allowing the language-processing modules run in parallel. No restrictions are placed a priori on the NLP modules apart of being able to consume and produce linguistic annotations following a given format. We show the feasibility of our approach by integrating two linguistic processing chains for English and Spanish. Moreover, we provide several scripts that allow building from scratch a whole distributed architecture that can be then easily installed and deployed onto a cluster of machines. The scripts and the NLP modules used in the paper are publicly available and distributed under free licenses. In the paper, we also describe a series of experiments carried out in the context of the NewsReader project with the goal of testing how the system behaves in different scenarios.
The Sufyānī in Early Islamic Kerygma: An Enquiry into His Origins and Early Development
Authors: SHADDEL MEHDY
Published: May 2017

The present study aims to contribute to the quest for the origins of belief in the eschatological figure of the Sufyānī, a matter of hot debate since the late nineteenth century. To this end, three different bodies of evidence are produced and analysed: reports indicating that the Sufyānī was, indeed, thought of as a redemptive personality in some Syrian quarters, traditions on him in the Muslim endtimes literature that contain an ex eventu pronouncement, and reports concerning the propaganda activities of the first Sufyānī claimant, Abū Muḥammad Ziyād ibn ʿAbd Allāh ibn Yazīd “al-Sufyānī”, in the historical record. Making use of hitherto unavailable sources and looking afresh at the previously studied sources, it is argued that the myth of the Sufyānī emerged during the counterrevolutionary revolt of Abū Muḥammad al-Sufyānī in 132ah, with vague residues of it traceable to his earlier military activities, against the Umayyad caliph Yazīd III, in 126ah.
Israel ‘Looks East:’ Besides India and China, Israel's Relations with Japan Seem to Flourish
Authors: AGDEMIR A. MURAT
Published: May 2017

Israel seeks to have good relations with most members of the international community, and establishes friendly relations with as many states as possible. As part of the Asian continent, Israel has been interested from its inception in good ties with China and India in particular. In this regard, besides India and China, another important Asian country with which Israel's relations are flourishing is Japan. Over recent years, the two countries’ relations have steadily progressed. Cooperation between the two countries could be productive, and both Israel and Japan have much to gain from developing a relationship. In this context, this article firstly gives a brief summary of Israel's developing relations with two important countries of Asia, India and China, then tries to explain the nature of the developing relations between Israel and Japan, and aims to point out possible future areas of cooperation.
India's Subnational Governments Foray into the International Arena
Authors: JAIN PURNENDRA , MAINI TRIDIVESH SINGH
Published: May 2017

Subnational diplomacy has come to life in India. Although still nowhere near in the same league as their counterparts in North America, Europe, parts of Asia, and even other developing countries, subnational governments (SNGs) in India are increasingly engaged abroad through involvement in regional and global affairs, questioning the central government on international matters, seeking foreign direct investment, organizing trade fairs and investment summits, leading economic missions, and hosting visiting dignitaries to showcase their jurisdiction's economic potential. These new and emerging actors on India's foreign affairs landscape are pluralizing and decentralizing India's foreign policy, albeit in a limited way and on small scale, as all states and territories are not as yet actively involved in foreign affairs. The paper argues that management of India's external relations is no longer informed or conducted simply by a select group of diplomats, high-ranking political leaders, and other specialist foreign policy advisers in New Delhi. Today subnational actors are becoming an essential component in India's foreign affairs. They are joining the huge traffic of SNGs from countries around the world that are linking with counterparts and others across national borders to serve the interests of local constituencies. It is a trend that is changing not only diplomatic roles, but also the nature of economic and political relationships among government actors worldwide.
A Study of Career Networks and the Geographical Characteristics of CCP's Top Political and Military Positions
Authors: LIN SHYH-CHYANG
Published: May 2017

This research study attempts to map out the career network of selected members from the Politburo and Central Military Commission (CMC) of the Chinese Communist Party (CCP), by adopting social network analysis techniques, calculating various network indices, and quantifying the value and importance of each position. The purpose of this research is to uncover the crucial positions leading to the CCP's political and military power center, as well as to how these positions interrelate. The results show the following. First, in the Jiang Zemin era (Jiang's era), Politburo members were mainly promoted from the central party committee or Central Government. Secondly, in the Hu Jintao era (Hu's era), key members from administrative regions started to step into the power center. Third, in the era of Xi Jinping (Xi's era), most Politburo members were promoted directly from administrative regions, especially from the coastal areas, such as Shanghai and Guangdong province. The research also indicates that being a military commander for Military Regions appears to provide experience necessary for promotion, whilst being an officer at the central military departments, such as a (Deputy) Chief General of Staff or Commander of Armed Services, is considered a major stepping stone. Although the succession trend defined by the innovative analysis of this research might not match the fixed model used by the CCP for its succession plan, under the circumstances of probable further institutionalization of the CCP, a quantified analysis of network position could be considered a more promising method of prediction.
THE VIRTUES OF A GOOD HISTORIAN IN EARLY IMPERIAL GERMANY: GEORG WAITZ'S CONTESTED EXAMPLE
Authors: PAUL HERMAN
Published: May 2017

Recent literature on the moral economy of nineteenth-century German historiography shares with older scholarship on Leopold von Ranke's methodological revolution a tendency to refer to “the” historical discipline in the third person singular. This would make sense as long as historians occupied a common professional space and/or shared a basic understanding of what it meant to be a historian. Yet, as this article demonstrates, in a world sharply divided over political and religious issues, historians found it difficult to agree on what it meant to be a good historian. Drawing on the case of Ranke's influential pupil Georg Waitz, whose death in 1886 occasioned a debate on the relative merits of the example that Waitz had embodied, this article argues that historians in early imperial Germany were considerably more divided over what they called “the virtues of the historian” than has been acknowledged to date. Their most important frame of reference was not a shared discipline but rather a variety of approaches corresponding to a diversity of models or examples (“scholarly personae,” in modern academic parlance), the defining features of which were often starkly contrasted. Although common ground beneath these disagreements was not entirely absent, the habit of late nineteenth-century German historians to position themselves between Waitz and Heinrich von Sybel, Ranke and Friedrich Christoph Dahlmann, or other pairs of proper names turned into models of virtue, suggests that these historians experienced their professional environment as characterized primarily by disagreement over the marks of a good historian.
Efficient 2-D leaky-wave antenna configurations based on graphene metasurfaces
Authors: Fuscaldo Walter , Burghignoli Paolo , Baccarelli Paolo , Galli Alessandro
Published: May 2017

Different configurations of leaky-wave antennas (LWAs) based on graphene metasurfaces are studied. The electronic properties of a graphene metasurface in the low THz range are investigated in details in order to discuss the reconfigurability features of the presented structures. Simple exact formulas for evaluating the ohmic losses related to the surface plasmon polariton (SPP) propagation along a suspended graphene sheet, and the relevant figures of merit of SPP propagating over a generic metasurface are given. Such formulas allow us to explain the low efficiency of reconfigurable antennas based on SPPs along graphene metasurfaces. Then, the radiative performance and relevant losses of graphene Fabry–Perot cavity antennas (FPCAs) based on non-plasmonic leaky waves (LWs) are investigated and compared with previous solutions based on SPPs. In particular, a single-layer structure, i.e. a grounded dielectric slab covered with a graphene metasurface, and a multilayered structure, i.e. a substrate–superstrate antenna in which the graphene metasurface is embedded at a suitable position within the substrate, are considered in detail. The results show that the proposed LW solutions in graphene FPCAs allow for considerably reducing the ohmic losses, thus significantly improving the efficiency of the proposed radiators.
MIMO antennas with diversity and mutual coupling reduction techniques: a review
Authors: Malviya Leeladhar , Panigrahi Rajib Kumar , Kartikeyan M. V.
Published: May 2017

Multiple input multiple output (MIMO) antenna is at core of the presently available wireless technologies. The design of MIMO antennas over a limited space requires various approaches of mutual coupling reduction, otherwise gain, efficiency, diversity gain, and radiation patterns will be severely affected. Various techniques have been reported in literature to control this degrading factor and to improve the performance of the MIMO antennas. In this review paper, we have carried out an extensive thorough investigation of diversity and mutual coupling (correlation) reduction techniques in compact MIMO antennas.
Chromosomal Aberrations in Monozygotic and Dizygotic Twins Versus Singletons in Denmark During 1968–2009
Authors: Kroeldrup Lone , Larsen Lisbeth A. , Fagerberg Christina , Hertz Jens M. , Christensen Kaare
Published: May 2017

Background:Hall (Embryologic development and monozygotic twinning.Acta Geneticae Medicae et Gemellologiae, Vol. 45, 1996, pp. 53–57) hypothesized that chromosomal aberrations can lead to monozygotic (MZ) twinning. However, twinning and chromosomal aberrations increase prenatal mortality and could reduce the prevalence of chromosomal aberrations in live-born twins. We compared prevalence proportion ratios (PPR) of chromosomal aberrations and trisomy 21 (T21) in live-born twins versus singletons born in Denmark during 1968–2009.Methods:We linked the Danish Twin Registry and a 5% random sample of all singletons to the Danish Cytogenetic Central Register and calculated PPR adjusted for maternal age for MZ, dizygotic (DZ), and all twins versus singletons. Zygosity was based on questionnaires or genetic markers.Results:No overall difference in risk of chromosomal aberrations or T21 in twins versus singletons was found. PPR in MZ and DZ twins was 0.87 (95% CI [0.60, 1.27]) and 1.05 (95% CI [0.88, 1.27]), respectively. For T21 there was a tendency to a lower prevalence in MZ twins compared to singletons (PPR: 0.29, 95% CI [0.07, 1.14]), whereas PPR was significantly increased in DZ twins (1.62, 95% CI [1.20, 2.19]). The observed proportion of MZ twin pairs among twin pairs with aberrations (0.22, 95% CI [0.16, 0.28]) was significantly lower than the proportion expected from the Weinberg method (0.32, 95% CI [CI, 0.26, 0.39]).Conclusion:Based on databases providing complete national coverage on twins with chromosomal aberrations, we found no overall difference in risk of chromosomal aberrations or T21 in twins versus singletons. Around conception twins may have an increased risk of chromosomal aberrations, but loss of especially MZ embryos could lead to similar risk among live-born twins and singletons.
Massively Parallel Sequencing (MPS) of Cell-Free Fetal DNA (cffDNA)
for Trisomies 21, 18, and 13 in Twin Pregnancies
Authors: Du Erqiu , Feng Chun , Cao Yuming , Yao Yanru , Lu Jing , Zhang Yuanzhen
Published: May 2017

Massively parallel sequencing (MPS) technology has become increasingly available
and has been widely used to screen for trisomies 21, 18, and 13 in singleton
pregnancies. This study assessed the performance of MPS testing of cell-free
fetal DNA (cffDNA) from maternal plasma for trisomies 21, 18, and 13 in twin
pregnancies. Ninety-two women with twin pregnancies were recruited. The results
were identified through karyotypes of amniocentesis or clinical examination and
follow-up of the neonates. Fluorescent in-situ hybridization was used to examine
the placentas postnatally in cases of false-positive results. The fetuses with
autosomal trisomy 21 (n = 2) and trisomy 15 (n
= 1) were successfully detected via MPS testing of cffDNA. There was one
false-positive for trisomy 13 (n = 1), and fluorescence in-situ
hybridization (FISH) identified confined placental mosaicism in this case. For
twin pregnancies undergoing second-trimester screening for trisomy, MPS testing
of cffDNA is feasible and can enhance the diagnostic spectrum of non-invasive
prenatal testing, which could effectively reduce invasive prenatal diagnostic
methods. In addition to screening for trisomy 21, 18, and 13 by cffDNA, MPS can
detect fetal additional autosomal trisomy. False-positive results cannot
completely exclude confined placental mosaicism.
Foxp3+ regulatory T cells maintain the bone marrow microenvironment for B cell lymphopoiesis
Authors: Pierini Antonio , Nishikii Hidekazu , Baker Jeanette , Kimura Takaharu , Kwon Hye-Sook , Pan Yuqiong , Chen Yan , Alvarez Maite , Strober William , Velardi Andrea , Shizuru Judith A. , Wu Joy Y. , Chiba Shigeru , Negrin Robert S.
Published: May 2017

Foxp3+ regulatory T cells (Treg cells) modulate the immune system and maintain self-tolerance, but whether they affect haematopoiesis or haematopoietic stem cell (HSC)-mediated reconstitution after transplantation is unclear. Here we show that B-cell lymphopoiesis is impaired in Treg-depleted mice, yet this reduced B-cell lymphopoiesis is rescued by adoptive transfer of affected HSCs or bone marrow cells into Treg-competent recipients. B-cell reconstitution is abrogated in both syngeneic and allogeneic transplantation using Treg-depleted mice as recipients. Treg cells can control physiological IL-7 production that is indispensable for normal B-cell lymphopoiesis and is mainly sustained by a subpopulation of ICAM1+ perivascular stromal cells. Our study demonstrates that Treg cells are important for B-cell differentiation from HSCs by maintaining immunological homoeostasis in the bone marrow microenvironment, both in physiological conditions and after bone marrow transplantation.
Quantum-coherent mixtures of causal relations
Authors: MacLean Jean-Philippe W. , Ried Katja , Spekkens Robert W. , Resch Kevin J.
Published: May 2017

Understanding the causal influences that hold among parts of a system is critical both to explaining that system’s natural behaviour and to controlling it through targeted interventions. In a quantum world, understanding causal relations is equally important, but the set of possibilities is far richer. The two basic ways in which a pair of time-ordered quantum systems may be causally related are by a cause-effect mechanism or by a common-cause acting on both. Here we show a coherent mixture of these two possibilities. We realize this nonclassical causal relation in a quantum optics experiment and derive a set of criteria for witnessing the coherence based on a quantum version of Berkson’s effect, whereby two independent causes can become correlated on observation of their common effect. The interplay of causality and quantum theory lies at the heart of challenging foundational puzzles, including Bell’s theorem and the search for quantum gravity.
Earliest signs of life on land preserved in ca. 3.5 Ga hot spring deposits
Authors: Djokic Tara , Van Kranendonk Martin J. , Campbell Kathleen A. , Walter Malcolm R. , Ward Colin R.
Published: May 2017

The ca. 3.48 Ga Dresser Formation, Pilbara Craton, Western Australia, is well known for hosting some of Earth’s earliest convincing evidence of life (stromatolites, fractionated sulfur/carbon isotopes, microfossils) within a dynamic, low-eruptive volcanic caldera affected by voluminous hydrothermal fluid circulation. However, missing from the caldera model were surface manifestations of the volcanic-hydrothermal system (hot springs, geysers) and their unequivocal link with life. Here we present new discoveries of hot spring deposits including geyserite, sinter terracettes and mineralized remnants of hot spring pools/vents, all of which preserve a suite of microbial biosignatures indicative of the earliest life on land. These include stromatolites, newly observed microbial palisade fabric and gas bubbles preserved in inferred mineralized, exopolymeric substance. These findings extend the known geological record of inhabited terrestrial hot springs on Earth by ∼3 billion years and offer an analogue in the search for potential fossil life in ancient Martian hot springs.
Ultra-large supramolecular coordination cages composed of endohedral Archimedean and Platonic bodies
Authors: Byrne Kevin , Zubair Muhammad , Zhu Nianyong , Zhou Xiao-Ping , Fox Daniel S. , Zhang Hongzhou , Twamley Brendan , Lennox Matthew J. , Düren Tina , Schmitt Wolfgang
Published: May 2017

Pioneered by Lehn, Cram, Peterson and Breslow, supramolecular chemistry concepts have evolved providing fundamental knowledge of the relationships between the structures and reactivities of organized molecules. A particular fascinating class of metallo-supramolecular molecules are hollow coordination cages that provide cavities of molecular dimensions promoting applications in diverse areas including catalysis, enzyme mimetics and material science. Here we report the synthesis of coordination cages with exceptional cross-sectional diameters that are composed of multiple sub-cages providing numerous distinctive binding sites through labile coordination solvent molecules. The building principles, involving Archimedean and Platonic bodies, renders these supramolecular keplerates as a class of cages whose composition and topological aspects compare to characteristics of edge-transitive {Cu2} MOFs with A3X4 stoichiometry. The nature of the cavities in these double-shell metal-organic polyhedra and their inner/outer binding sites provide perspectives for post-synthetic functionalizations, separations and catalysis. Transmission electron microscopy studies demonstrate that single molecules are experimentally accessible.
Charting organellar importomes by quantitative mass spectrometry
Authors: Peikert Christian D. , Mani Jan , Morgenstern Marcel , Käser Sandro , Knapp Bettina , Wenger Christoph , Harsman Anke , Oeljeklaus Silke , Schneider André , Warscheid Bettina
Published: May 2017

Protein import into organelles is essential for all eukaryotes and facilitated by multi-protein translocation machineries. Analysing whether a protein is transported into an organelle is largely restricted to single constituents. This renders knowledge about imported proteins incomplete, limiting our understanding of organellar biogenesis and function. Here we introduce a method that enables charting an organelle’s importome. The approach relies on inducible RNAi-mediated knockdown of an essential subunit of a translocase to impair import and quantitative mass spectrometry. To highlight its potential, we established the mitochondrial importome of Trypanosoma brucei, comprising 1,120 proteins including 331 new candidates. Furthermore, the method allows for the identification of proteins with dual or multiple locations and the substrates of distinct protein import pathways. We demonstrate the specificity and versatility of this ImportOmics method by targeting import factors in mitochondria and glycosomes, which demonstrates its potential for globally studying protein import and inventories of organelles.
Amorphous nickel-cobalt complexes hybridized with 1T-phase molybdenum disulfide via hydrazine-induced phase transformation for water splitting
Authors: Li Haoyi , Chen Shuangming , Jia Xiaofan , Xu Biao , Lin Haifeng , Yang Haozhou , Song Li , Wang Xun
Published: May 2017

Highly active and robust eletcrocatalysts based on earth-abundant elements are desirable to generate hydrogen and oxygen as fuels from water sustainably to replace noble metal materials. Here we report an approach to synthesize porous hybrid nanostructures combining amorphous nickel-cobalt complexes with 1T phase molybdenum disulfide (MoS2) via hydrazine-induced phase transformation for water splitting. The hybrid nanostructures exhibit overpotentials of 70 mV for hydrogen evolution and 235 mV for oxygen evolution at 10 mA cm−2 with long-term stability, which have superior kinetics for hydrogen- and oxygen-evolution with Tafel slope values of 38.1 and 45.7 mV dec−1. Moreover, we achieve 10 mA cm−2 at a low voltage of 1.44 V for 48 h in basic media for overall water splitting. We propose that such performance is likely due to the complete transformation of MoS2 to metallic 1T phase, high porosity and stabilization effect of nickel-cobalt complexes on 1T phase MoS2.
Erratum: Origin and structure of polar domains in doped molecular crystals
Authors: Meirzadeh E. , Azuri I. , Qi Y. , Ehre D. , Rappe A. M. , Lahav M. , Kronik L. , Lubomirsky I.
Published: May 2017

Nature Communications 7: Article number: 13351 (2016); Published: 8 November 2016; Updated: 9 May 2017 In Fig. 1 of this Article, the top crystallographic face of the crystal was inadvertently mislabelled ‘(0͞10)’ during the production process. It should read ‘(010)’. The correct version of Fig. 1 appears below.
Sleep disorder, an independent risk associated with arterial stiffness in menopause
Authors: Zhou Yang , Yang Ruwei , Li Changbin , Tao Minfang
Published: May 2017

As women age and go through menopause, they suffer a higher incidence of sleep disorder, cardiovascular morbidity and mortality. In addition, evidences suggested that sleep disorder was an important pathological indicator for coronary heart disease. However, the relationship between different menopausal status, sleep disorder and cardiovascular diseases was unclear. Thus, we aim to assess the association between sleep disorder with arterial stiffness in females of 40–60 years free of cardiovascular diseases through self-administered Pittsburgh Sleep Quality Index (PSQI) and brachial-ankle pulse wave velocity (baPWV). Logistic regression revealed that sleep disorder (PSQI score ≥ 8) was an independent indicator for higher risk of elevated arterial stiffness (baPWV ≥ 1465.5 cm/s, upper tertile) beyond other established cardiovascular confounders in peri-postmenopause (OR 2.83, 95% confidence interval (CI) 2.00–4.00, p < 0.001), but not in premenopause (OR 1.67, 95% CI 0.71–3.90, p = 0.223). Collectively, it clearly indicates that sleep disorder in menopausal women is of prominent value to predict arterial stiffness.
Increased leukotoxin production: Characterization of 100 base pairs within the 530 base pair leukotoxin promoter region of Aggregatibacter actinomycetemcomitans
Authors: Sampathkumar Vandana , Velusamy Senthil Kumar , Godboley Dipti , Fine Daniel H.
Published: May 2017

Aggregatibacter actinomycetemcomitans leukotoxin (LtxA) is a major virulence factor that kills leukocytes permitting it’s escape from host immune surveillance. A. actinomycetemcomitans strains can produce high or low levels of toxin. Genetic differences reside in the “so called JP2” ltxA promoter region. These hyper-leukotoxin producing strains with the 530 bp deletion have been studied in detail. However, regions contained within the 530 bp deletion that could be responsible for modulation of leukotoxin production have not been defined. Here, we report, for the first time, on regions within the 530 bp that are responsible for high-levels of ltxA expression. We constructed a deletion of 530 bps in a primate isolate of A. actinomycetemcomitans, which produced leukotoxin equivalent to the JP2 strain. We then constructed sequential deletions in regions that span the 530 bps. Results indicated that expression of the ltxA transcript was reduced by a potential transcriptional terminator in promoter region 298 to 397 with a ΔG = −7.9 kcal/mol. We also confirmed previous findings that transcriptional fusion between the orfX region and ltxC increased ltxA expression. In conclusion, we constructed a hyper-leukotoxin producing A. actinomycetemcomitans strain and identified a terminator located in the promoter region extending from 298–397 that alters ltxA expression.
Cells reprogramming to stem cells inhibit the reprogramming of adjacent cells in the moss Physcomitrella patens
Authors: Sato Yoshikatsu , Sugimoto Nagisa , Hirai Tadayoshi , Imai Akihiro , Kubo Minoru , Hiwatashi Yuji , Nishiyama Tomoaki , Hasebe Mitsuyasu
Published: May 2017

Under certain circumstances differentiated cells can be reprogrammed to form stem cells in land plants, but only a portion of the cells reprograms successfully. A long-distance inhibitory signal from reprogrammed cells to surrounding cells has been reported in some ferns. Here we show the existence of anisotropic inhibitory signal to regulate stem cell formation in the moss Physcomitrella patens. When single cells were isolated from a gametophore leaf, over 90% of them were reprogrammed to stem cells with characteristic nuclear expansion. By contrast, when two adjacent cells were isolated, the nuclei of both cells expanded, but successful reprogramming of both cells occurred only in approximately one fifth of the pairs. When three aligned cells were isolated, the reprogramming rate of both edge cells decreased with a living middle cell but did not with a dead middle cell. Furthermore, unequal conversion into stem cells was more prominent in cell pairs aligned parallel to the proximal-distal leaf axis than in those perpendicular to the axis. This study gives an insight into the role of the inhibitory signal in development and evolution as well as the efficient stem cell induction from differentiated cells.
Mycoviruses of an endophytic fungus can replicate in plant cells: evolutionary implications
Authors: Nerva L. , Varese G. C. , Falk B. W. , Turina M.
Published: May 2017

So far there is no record of a specific virus able to infect both fungal and plant hosts in nature. However, experimental evidence shows that some plant virus RdRPs are able to perform replication in trans of genomic or DI RNAs in the yeast Saccharomyces cerevisiae. Furthermore, tobacco mosaic virus was recently shown to replicate in a filamentous ascomycetous fungus. Thus, at least experimentally, some plant viruses can infect some fungi. Endophytic fungi have been reported from many plants and several of these fungi have been shown to contain viruses. Here we tested if mycoviruses derived from a marine plant endophyte can replicate in plant cells. For this purpose, we used partially purified viral particles from isolate MUT4330 of Penicillium aurantiogriseum var. viridicatum which harbors six virus species, some having dsRNA and some positive-strand ssRNA genomes. These were transfected into three distinct plant protoplast cell systems. Time-course analysis of absolute RNA accumulation provided for the first time evidence that viruses of two species belonging to the Partitiviridae and Totiviridae families, can replicate in plant cells without evidence of host adaptation, i.e, changes in their nucleotide sequence.
Design and evaluation of EphrinA1 mutants with cerebral protective effect
Authors: Zhu Yuanjun , Gao Yuanqing , Zheng Danping , Shui Mengyang , Yu Kuai , liu Xiaoyan , Lin Yuan , Su Li , Yang Wenxing , Wang Yinye
Published: May 2017

The activation of EphA2 receptor by its natural ligand EphrinA1 causes blood brain barrier dysfunction, and inactivation of EphA2 reduces BBB damage in ischemic stroke. Thus, EphA2 targeted antagonists may serve as neuroprotective agents. We engineered four mutants of EphrinA1, EM1, EM2, EM3 and EM4, respectively. The computational analysis showed that these four mutants were capable of interacting with EphA2. Their potential neuroprotective effects were examined in mouse focal ischemia/reperfusion (I/R) model. EM2 exhibited strong neuroprotective effects, including reduced brain infarct volume, neuronal apoptosis, cerebral edema, and improved neurological scores. The EM2-mediated protection was associated with a comparative decrease in BBB leakage, inflammatory infiltration, and higher expression levels of tight junction proteins, such as zonula occludens-1 and Occludin. I/R-induced high expression of Rho-associated protein kinase 2 (ROCK2) was down-regulated after EM2 treatment. Moreover, EM2 reduced agonist doxazosin-induced EphA2 phosphorylation and cells rounding in PC3 cells, indicating EphA2-antagonizing activity of EM2. These finding provided evidences of the neuroprotection of EphA2 antagonist and a novel approach for ischemic stroke treatment. These results also suggested that a receptor agonist can be switched to an antagonist by substituting one or more relevant residues.