bluuebunny/crossref_metadata_2025 huggingface dataset:
A Systematic Analysis of the Clinical Outcome Associated with Multiple Reclassified Desmosomal Gene Variants in Arrhythmogenic Right Ventricular Cardiomyopathy Patients
Authors: Nagyova Emilia , Hoorntje Edgar T. , te Rijdt Wouter P. , Bosman Laurens P. , Syrris Petros , Protonotarios Alexandros , Elliott Perry M. , Tsatsopoulou Adalena , Mestroni Luisa , Taylor Matthew R. G. , Sinagra Gianfranco , Merlo Marco , Wada Yuko , Horie Minoru , Mogensen Jens , Christensen Alex H. , Gerull Brenda , Song Lei , Yao Yan , Fan Siyang , Saguner Ardan M. , Duru Firat , Koskenvuo Juha W. , Cruz Marino Tania , Tichnell Crystal , Judge Daniel P. , Dooijes Dennis , Lekanne Deprez Ronald H. , Basso Cristina , Pilichou Kalliopi , Bauce Barbara , Wilde Arthur A. M. , Charron Philippe , Fressart Véronique , van der Heijden Jeroen F. , van den Berg Maarten P. , Asselbergs Folkert W. , James Cynthia A. , Jongbloed Jan D. H. , Harakalova Magdalena , van Tintelen J. Peter
Published: July 2023

The presence of multiple pathogenic variants in desmosomal genes (DSC2, DSG2, DSP, JUP, and PKP2) in patients with arrhythmogenic right ventricular cardiomyopathy (ARVC) has been linked to a severe phenotype. However, the pathogenicity of variants is reclassified frequently, which may result in a changed clinical risk prediction. Here, we present the collection, reclassification, and clinical outcome correlation for the largest series of ARVC patients carrying multiple desmosomal pathogenic variants to date (n = 331). After reclassification, only 29% of patients remained carriers of two (likely) pathogenic variants. They reached the composite endpoint (ventricular arrhythmias, heart failure, and death) significantly earlier than patients with one or no remaining reclassified variant (hazard ratios of 1.9 and 1.8, respectively). Periodic reclassification of variants contributes to more accurate risk stratification and subsequent clinical management strategy.
Feasibility study for the manufacturing of hybrid pinion shafts with the cross-wedge rolling process
Authors: Merkel Paulina , Budde Laura , Grajczak Jan , Nowroth Christian , Prasanthan Vannila , Kriwall Mareile , Lammers Marius , Nothdurft Sarah , Hermsdorf Jörg , Twiefel Jens , Overmeyer Ludger , Kaierle Stefan , Wallaschek Jörg , Breidenstein Bernd , Behrens Bernd-Arno , Stonis Malte
Published: July 2023

The Collaborative Research Center 1153 is investigating an innovative process chain for the production of hybrid components. The hybrid workpieces are first joined and then formed by cross-wedge rolling. Pinion shafts were manufactured to investigate the behavior of the joining zone under increased complexity of the forming process. For this purpose, six types of workpieces produced by three types of joining processes were formed into pinion shafts. The reference process provides a shaft with a smooth bearing seat. It was found that the increased complexity did not present any challenges compared to the reference processes. A near-net shape geometry was achieved for the pinions made of steel.
Numerical and experimental analysis of the isothermal high temperature pneumoforming process
Authors: Kamaliev Mike , Flesch Jan , Grodotzki Joshua , Tekkaya A. Erman
Published: July 2023

The isothermal high temperature pneumoforming process to form tubes at constant elevated temperatures by means of internal pressure is investigated. Two materials, a ferritic (X2CrTiNb18) and a martensitic stainless steel (X12Cr13) are used for the investigations. The required material characterization is performed at the temperature and strain rate of the actual process. A new method for quantifying thermal softening via the time-dependent decrease in static yield stress is presented. At a temperature of 1000 °C, the static yield stress decreases by 50% within 100 s for both materials. The numerical models are validated on the basis of the formed geometry and used to study the influence of maximum internal pressure, axial feed, holding time under load and die edge length on the final part geometry. It was observed, that with higher internal pressures and longer holding times smaller corner radii are formed for both materials. In contrast, a superimposed axial feed as well as the effective friction coefficient have a negligible influence on the formed geometry. With an increasing die edge length, smaller radii are formed with the ferritic stainless steel numerically and experimentally. By contrast, for the martensitic stainless steel, larger radii are observed numerically. Experimentally, the limited formability of these tubes weld seam becomes apparent. Based on the findings, process windows depending on the process parameters internal pressure and die edge length were derived. Numerically, forming limit curves of tubular semi-finished products under comparable conditions serve as a failure criterion. Good agreement with experiments was observed.
Rethinking Risk in Adults’ Engagement with Sexual Digital Imagery
Authors: Power Jennifer , Dowsett Gary W. , Waling Andrea , James Alexandra , Moor Lily , Shackleton Nicole , Farrell Anne-Maree
Published: July 2023

Introduction

Camera-equipped smartphones and other devices allow people to capture and share images directly with others in ways that are spontaneous, instant and relatively inexpensive. Such sharing is a common part of modern sexual intimacies, despite media and educational discourses warning of potential risks.

Methods

This paper reports on a qualitative study in which we interviewed 23 Australian adults about the ways in which they used with digital sexual imagery in their sex lives. The study aimed to explore participants’ experiences of digital sexual self-image creation and sharing and the ways discourses of risk and safety shape these experiences.

Results

Findings showed that participants tended to view the creation and exchange of sexual images as a form of sexual play that built intimacy, sexual tension and eroticism into their relationships and expanded their sexual and relationship experiences in positive ways. Participants were aware that sending sexual or nude images left them vulnerable to potential harm or unwanted sexual exposure and were concerned that some people would view such practices as foolish or irresponsible given these risks held such high profile. These concerns meant participants were often secretive about their experiences although the sense of risk and vulnerability in these exchanges enhanced intimacy or eroticism for some participants.

Conclusions

Educational approaches to managing digital risk should recognise that people often have positive digital sexual experiences that can lead them to disengage from fear-based messaging.

Policy implications

As digital technologies evolve, their integration with human sexual intimacy will continue to change and develop in unforeseen ways. Educational and regulatory responses will require ongoing scrutiny and innovation to acknowledge users’ positive experiences and desires while responding to risks and challenges.
Hybrid solder joints: the effect of nanosized ZrO2 particles on morphology of as-reflowed and thermally aged Sn–3.5Ag solder joints
Authors: Wodak Irina , Yakymovych Andriy , Svec Peter , Orovcik Lubomir , Khatibi Golta
Published: July 2023

The main number of current researches has been focused on the microstructure and mechanical properties of the Sn-based Sn–Ag–Cu-based solders, while various kinds of nanosized particles have been added. The synthesis and handling of ceramic nanosized powder are much easier than of metal nanoparticles. In addition, metal nanoparticles solved in solder joints during the soldering process or by thermal aging could behave as an alloying element similar to bulk metal additions, while ceramic nanoparticles retain their chemically inactive behavior in various thermal, thermo-mechanical, and electrical constraints. In some cases, the solved metal nanosized inclusions could increase the growth kinetics of the present intermetallic phases or even create new phases, which leads to more complexity in the predictions and simulations of chemical processes in the solder joints. Based on the assertions mentioned above, ceramic nanosized particles are industrially more favorable as reinforcing inclusions. On the other hand, there is no direct comparison in the literature between Sn-based Sn–Ag–Cu and Sn–Ag solder joints with similar ceramic nanoinclusions based on microstructural features and mechanical properties. In the present research, the Cu/flux + NPs/SAC/flux + NPs/Cu solder joints were produced with a nominal amount of 0.2 wt%, 0.5 wt%, and 1.0 wt% nanosized ZrO2 powder. The solder joints prepared via the above-described method are called in the literature as hybrid solder joints. The microstructure of the as-reflowed and thermally aged samples has been studied, especially at the interface solder/substrate. It has been shown that the minor additions of ZrO2 NPs lead to a decrease in the thickness of the Cu6Sn5 interfacial layer in the as-reflowed solder joints and a reduction in the growth kinetics of this layer, while the Cu3Sn interfacial IMC layer remains practically unaffected. Similar investigations were performed in our previous study but for both the hybrid and nanocomposite Sn–3.0Ag–0.5Cu solder joints. A comparative analysis of the impact of the ZrO2 nanoinclusions on the hybrid solder joints using Sn–3.5Ag and Sn–3.0Ag–0.5Cu has been performed.
Guiding the design and implementation of interactive public displays in educational settings
Authors: Müller Maximilian , Otero Nuno , Milrad Marcelo
Published: July 2023

Interactive Public Displays (IPD) enable new ways of interaction as well as communication channels, extending online communities into physical places and supporting a culture of participation. While educational environments have seen how new digital technologies can enhance learning activities beyond the traditional classroom context, the use of IPDs is still an area insufficiently explored. This paper proposes a set of design goals for the implementation and deployment of engaging interactive public display applications in educational settings. Based on findings from a series of design workshops and two deployment studies in authentic settings, seven design goals were identified and defined. The design goals provide clear guidelines for the design of IPDs for schools by making design teams and stakeholders focus on factors fostering user adoption, social interactions and collaboration. The design goals also opened up paths for further explorations regarding display awareness, level of commitment in interactions, the displays’ integration into structured activities, and display management at the educational institutions.
Learning graph structures with transformer for weakly supervised semantic segmentation
Authors: Sun Wanchun , Feng Xin , Ma Hui , Liu Jingyao
Published: July 2023

Weakly supervised semantic segmentation (WSSS) is a challenging task of computer vision. The state-of-the-art semantic segmentation methods are usually based on the convolutional neural network (CNN), which mainly have the drawbacks of inability to explore the global information correctly and failure to activate potential object regions. To avoid such drawbacks, the transformer approach is explored in the WSSS task, but no effective semantic association between different patch tokens can be determined in the transformer. To address this issue, inspired by the graph convolutional network (GCN), this paper proposes a graph structure to learn the semantic category relationships between different blocks in the vector sequence. To verify the effectiveness of the proposed method in this paper, a large number of experiments were conducted on the publicly available PASCAL VOC2012 dataset. The experimental results show that our proposed method achieves significant performance improvement in the WSSS task and outperforms other state-of-the-art transformer-based methods.
Solvability of One-Dimensional Semilinear Hyperbolic Systems and Sine-Gordon Equation
Authors: Huh Hyungjin
Published: July 2023

We study semilinear hyperbolic Eq. (1.1). We derive an explicit solution representation for some nonlinear terms F and G. For other nonlinear terms, it is shown that the solutions of the equations are related with the variable coefficient sine-Gordon equation.
Assessment of global and local neural network’s performance for model-free estimation of flow angles
Authors: Lerro A. , de Pasquale L.
Published: July 2023

A synthetic flow angle sensor, able to estimate angle-of-attack and angle-of-sideslip, can exploit different methods to solve a set of equations modelling data fusion from other onboard systems. In operative scenarios, measurements used for data fusion are characterised by several uncertainties that would significantly affect the synthetic sensor performance. The off-line use of neural networks is not a novelty to model deterministic synthetic flow angle sensors and to mitigate issues arising from real flight applications. A common practice is to train the neural network with corrupted data that are representative of uncertainties of the current application. However, this approach requires accurate tuning on the target aircraft and extensive flight test campaigns, therefore, making the neural network tightly dependent on the specific aircraft. In order to overcome latter issues, this work proposes the use of neural networks to solve a model-free scheme, derived from classical flight mechanics, that is independent from the target aircraft, flight regime and avionics. It is crucial to make use of a training dataset that is not related to any specific aircraft or avionics to preserve the generality of the scheme. Under these circumstances, global and local neural networks are herein compared with an iterative method to assess the neural capabilities to generalise the proposed model-free solver. The final objective of the present work, in fact, is to select the neural technique that can enable a flow angle synthetic sensor to be used on board any flying body at any flight regime without any further training sessions.
Selecting robust features for machine-learning applications using multidata causal discovery
Authors: S. Saranya Ganesh , Beucler Tom , Tam Frederick Iat-Hin , Gomez Milton S. , Runge Jakob , Gerhardus Andreas
Published: July 2023

Robust feature selection is vital for creating reliable and interpretable machine-learning (ML) models. When designing statistical prediction models in cases where domain knowledge is limited and underlying interactions are unknown, choosing the optimal set of features is often difficult. To mitigate this issue, we introduce a multidata (M) causal feature selection approach that simultaneously processes an ensemble of time series datasets and produces a single set of causal drivers. This approach uses the causal discovery algorithms PC

$ {}_1 $

or PCMCI that are implemented in the Tigramite Python package. These algorithms utilize conditional independence tests to infer parts of the causal graph. Our causal feature selection approach filters out causally spurious links before passing the remaining causal features as inputs to ML models (multiple linear regression and random forest) that predict the targets. We apply our framework to the statistical intensity prediction of Western Pacific tropical cyclones (TCs), for which it is often difficult to accurately choose drivers and their dimensionality reduction (time lags, vertical levels, and area-averaging). Using more stringent significance thresholds in the conditional independence tests helps eliminate spurious causal relationships, thus helping the ML model generalize better to unseen TC cases. M-PC

$ {}_1 $

with a reduced number of features outperforms M-PCMCI, noncausal ML, and other feature selection methods (lagged correlation and random), even slightly outperforming feature selection based on explainable artificial intelligence. The optimal causal drivers obtained from our causal feature selection help improve our understanding of underlying relationships and suggest new potential drivers of TC intensification.
Risk of fibromyalgia in patients with primary muscle tension dysphonia: a possible local manifestation of a musculoskeletal systemic disorder
Authors: Hamdan Abdul-Latif , Alam Elie , Feghali Patrick A R , Fadel Charbel , Jabbour Christopher , Ghanem Anthony
Published: July 2023

Objective

To investigate the risk of fibromyalgia in patients with primary muscle tension dysphonia.

Methods

A retrospective review was conducted of patients with primary muscle tension dysphonia, diagnosed based on history of dysphonia with evidence of laryngeal muscle tension on examination. Fibromyalgia was assessed using the Fibromyalgia Rapid Screening Tool (‘FiRST’).

Results

Fifty patients were enrolled: 25 with primary muscle tension dysphonia (study group) and 25 matched controls. The mean age of the study group was 50.7 ± 15.2 years versus 49.5 ± 18.6 years for the controls, with a male to female ratio of 3:2 for both groups. Fifty-six per cent tested positive for fibromyalgia in the study group versus 4 per cent in the controls (p < 0.001). The mean Voice Handicap Index 10 score in the study group was significantly higher for those who screened positive for fibromyalgia compared to those who screened negative. There was a positive, strong point-biserial correlation between Fibromyalgia Rapid Screening Tool and Voice Handicap Index 10 scores (r = 0.39; p = 0.09).

Conclusion

These results suggest that fibromyalgia is a significant co-morbid condition in primary muscle tension dysphonia.
Instability of axisymmetric flow in thermocapillary liquid bridges: Kinetic and thermal energy budgets for two-phase flow with temperature-dependent material properties
Authors: Stojanović Mario , Romanò Francesco , Kuhlmann Hendrik C.
Published: July 2023

In numerical linear stability investigations, the rates of change of the kinetic and thermal energy of the perturbation flow are often used to identify the dominant mechanisms by which kinetic or thermal energy is exchanged between the basic and the perturbation flow. Extending the conventional energy analysis for a single-phase Boussinesq fluid, the energy budgets of arbitrary infinitesimal perturbations to the basic two-phase liquid–gas flow are derived for an axisymmetric thermocapillary bridge when the material parameters in both phases depend on the temperature. This allows identifying individual transport terms and assessing their contributions to the instability if the basic flow and the critical mode are evaluated at criticality. The full closed-form energy budgets of linear modes have been derived for thermocapillary two-phase flow taking into account the temperature dependence of all thermophysical parameters. The influence of different approximations to the temperature dependence on the linear stability boundary of the axisymmetric flow in thermocapillary liquid bridges is tested regarding their accuracy. The general mechanism of symmetry breaking turns out to be very robust.
Fill level measurement of low-permittivity material using an M-sequence UWB radar
Authors: Wegner Tim Erich , Gebhardt Stefan , Del Galdo Giovanni
Published: July 2023

Due to increasingly complex and automated manufacturing processes, the demands on the control parameters of these processes are also increasing. One parameter is the fill quantity of, e.g., liquids in production plants, whose precise determination is of ever-growing importance. Up to now, the exact level of determination under difficult conditions, such as high ambient temperatures, has been a particular challenge. This paper demonstrates a novel method by which an M-sequence UWB radar can determine levels of low-permittivity materials in small metal containers. For this purpose, hot melt is used as an example. Thus, the influence of large temperature differences on the long-term stability of level measurement can also be investigated. The measurements show that the level of hot melt can be measured to be long-term stable with an accuracy of better than 3 mm. Furthermore, the precise determination of the empty state is highly important for many applications. For this reason, this paper shows a method for determining the empty state without complex calibration procedures. For the empty level indication, an accuracy of up to 0.5 mm could be achieved for molten hot glue and 3% of the tank volume, independent of the shape or aggregate state of the medium.
Stone Chemistry Records Pacific Migration
Authors: Hasler Caroline
Published: July 2023

Scientists used the chemistry of stone artifacts to trace human migration in the Pacific, revealing evidence of long voyages and cultural exchange.
Expected Geomagnetically Induced Currents in the Spanish Islands Power Transmission Grids
Authors: Torta J. M. , Marsal S. , Piña‐Varas P. , Hafizi R. , Martí A. , Campanyà J. , Canillas‐Pérez V. , Curto J. J. , Ledo J. , Queralt P. , Marcuello A.
Published: July 2023

The aim of this study is to evaluate the geomagnetically induced current (GIC) hazard in the power networks of the Canary and Balearic archipelagos. This is done in order to strictly complete the detailed assessment at national level of the power transmission system of mainland Spain, including the 400 and 220 kV levels. We have constructed models for the grids in each of the individual systems and used resistivity models of the lithosphere for each group of islands, from which we have calculated the surface impedances. The respective models of electrical admittances of the grids have been combined with the geoelectric field derived from the convolution of the recorded (or expected in an extreme scenario) geomagnetic storms and the impedances calculated from the geoelectrical models to derive the expected GICs in the power lines, substations, and transformers. The low geomagnetic latitude of the Canary Islands combined with the small size of their power networks, makes them one of the least likely electrified locations to record significant GICs, with less than 3 A for the 100‐year return period. Even the 13 A that could be reached for the upper limit of the 95% confidence interval at the 500‐year return period does not seem likely to have a significant impact. The Balearic Islands, being at higher latitude and with a system length of approximately 300 km, including alternating current power lines connecting the Islands, shows GIC signals of moderate amplitude with up to 40 A for the 100‐year return period.
Contribution of Fresh Submarine Groundwater Discharge to the Gulf of Alaska
Authors: Russo A. A. , Boutt D. F. , Munk L. A. , Jenckes J.
Published: July 2023

High latitude mountain environments are experiencing disproportionately adverse effects from climate change. The Gulf of Alaska (GoA) region is an embodiment of this change, particularly concerning a shifting hydrologic balance. Even so, the magnitude and contribution of fresh submarine groundwater discharge (fresh SGD) remains virtually unexplored within the region, though it has gained increasing attention globally due to its chemical significance and influence on coastal ecosystems. Here we provide the first regional estimates of fresh SGD to the GoA using two established water balance approaches. This is an effective way to distinguish the contribution of terrestrially derived fresh SGD, rather than the more commonly quantified total SGD which includes discharge that is driven by marine forces such as sea‐level oscillations and density gradients. We compare the approaches and assess their capabilities in computing the magnitude of fresh SGD over a large regional scale. Mean annual fresh SGD flux ranges between 26.5 and 86.8 km3 yr−1 to the GoA, equivalent to 3.5%–11.4% of the total freshwater discharge. Contributions are highest in the Southeastern panhandle and lowest in the Cook Inlet basin, with the highest area normalized contribution occurring in the Prince William Sound. Fresh SGD exhibits high spatial and temporal variability throughout the region. Although freshwater discharge to the GoA is investigated considerably, the importance of fresh SGD has, thus far, been overlooked.
Lanthanide complexes with d-f transition: new emitters for single-emitting-layer white organic light-emitting diodes
Authors: Fang Peiyu , Huo Peihao , Wang Liding , Zhao Zifeng , Yu Gang , Huang Yanyi , Bian Zuqiang , Liu Zhiwei
Published: July 2023

White organic light-emitting diodes (WOLEDs) is a new generation of lighting technology and has stimulated wide-ranging studies. Despite the advantage of simple device structure, single-emitting-layer WOLEDs (SEL-WOLEDs) still face the challenges of difficult material screening and fine energy level regulation. Herein, we report efficient SEL-WOLEDs with a sky-blue emitting cerium(III) complex Ce-TBO2Et and an orange-red emitting europium(II) complex Eu(Tp2Et)2 as the emitters, showing a maximum external quantum efficiency of 15.9% and Commission Internationale de l’Eclairage coordinates of (0.33, 0.39) at various luminances. Most importantly, the electroluminescence mechanism of direct hole capture and hindered energy transfer between the two emitters facilitate a manageable weight doping concentration of 5% for Eu(Tp2Et)2, avoiding the low concentration (<1%) of the low-energy emitter in typical SEL-WOLEDs. Our results indicate that d-f transition emitters may circumvent fine energy level regulation and provide development potential for SEL-WOLEDs.
Extreme summers impact cropland and grassland soil microbiomes
Authors: Bei Qicheng , Reitz Thomas , Schnabel Beatrix , Eisenhauer Nico , Schädler Martin , Buscot François , Heintz-Buschart Anna
Published: July 2023

The increasing frequency of extreme weather events highlights the need to understand how soil microbiomes respond to such disturbances. Here, metagenomics was used to investigate the effects of future climate scenarios (+0.6 °C warming and altered precipitation) on soil microbiomes during the summers of 2014–2019. Unexpectedly, Central Europe experienced extreme heatwaves and droughts during 2018–2019, causing significant impacts on the structure, assembly, and function of soil microbiomes. Specifically, the relative abundance of Actinobacteria (bacteria), Eurotiales (fungi), and Vilmaviridae (viruses) was significantly increased in both cropland and grassland. The contribution of homogeneous selection to bacterial community assembly increased significantly from 40.0% in normal summers to 51.9% in extreme summers. Moreover, genes associated with microbial antioxidant (Ni-SOD), cell wall biosynthesis (glmSMU, murABCDEF), heat shock proteins (GroES/GroEL, Hsp40), and sporulation (spoIID, spoVK) were identified as potential contributors to drought-enriched taxa, and their expressions were confirmed by metatranscriptomics in 2022. The impact of extreme summers was further evident in the taxonomic profiles of 721 recovered metagenome-assembled genomes (MAGs). Annotation of contigs and MAGs suggested that Actinobacteria may have a competitive advantage in extreme summers due to the biosynthesis of geosmin and 2-methylisoborneol. Future climate scenarios caused a similar pattern of changes in microbial communities as extreme summers, but to a much lesser extent. Soil microbiomes in grassland showed greater resilience to climate change than those in cropland. Overall, this study provides a comprehensive framework for understanding the response of soil microbiomes to extreme summers.
Influence of heterospecifics on mesocarnivore behaviour at shared scavenging opportunities in the Canadian Rocky Mountains
Authors: Bell Elicia , Fisher Jason T. , Darimont Chris , Hart Henry , Bone Christopher
Published: July 2023

In seasonal environments, the ability of mustelid species to acquire carrion—a dietary resource heavily depended upon—is driven by a collection local habitat characteristics and competition dynamics. In resource-scarce winter, sympatric mesocarnivores must balance energetic rewards of carrion with avoiding antagonistic interactions with conspecifics. We examined scavenging interactions among three mustelid species in the northern Canadian Rocky Mountains. Camera traps (n = 59) were baited with carrion during winter between 2006 to 2008. Spatial and temporal dimensions of scavenger behaviour (i.e., carcass use) were evaluated using a multi-model approach, which enabled us to recognize potentially adaptive behavioural mechanisms for mitigating competition at carcass sites. Best performing models indicated that carrion site use is governed by a combination of competition threats and environmental factors. A decrease in scavenging with increasing snow depth was observed across all species. Mustelids adopted a host of adaptive behavioural strategies to access shared scavenging opportunities. We found evidence that wolverine (Gulo gulo) and American marten (Martes americana) segregate in space but temporally tracked one another. Short-tailed weasel (Mustela erminea) scavenging decreased with greater site use by marten. Carcass availability across a spatially complex environment, as well as spatial–temporal avoidance strategies, can facilitate carrion resource partitioning.
Identification and elucidation of cross talk between SLAM Family Member 7 (SLAMF7) and Toll-like receptor (TLR) pathways in monocytes and macrophages
Authors: Choe Uyory , Pham Quynhchi , Kim Young S. , Yu Liangli , Wang Thomas T. Y.
Published: July 2023

To further elucidate the expression, regulation and function of Signaling Lymphocytic Activation Molecule Family (SLAMF) protein members in human monocytes and macrophages. Un-differentiated monocytic THP-1 cell (u-THP-1) and differentiated THP-1 macrophage (d-THP-1) were used as culture models in the study. Responses of cells to the differentiation agents phorbol ester (25 ng/ml) and TLR (Toll-like receptor) ligands were assessed. RT-PCR and Western blot analysis were used to determine mRNA and protein level. Pro-inflammatory cytokine mRNA expression levels and phagocytosis were used as functional markers. Data analyzed using t-test, one-way or two-way ANOVA followed by post hoc test. SLAMFs were differentially expressed in THP-1 cells. Differentiation of u-THP-1 to d-THP-1 led to significantly higher SLAMF7 mRNA and protein levels than other SLAMF. In addition, TLR stimuli increased SLAMF7 mRNA expression but not protein expression. Importantly, SLAMF7 agonist antibody and TLR ligands synergistically increased the mRNA expression levels of IL-1β, IL-6 and TNF-α, but had no effect on phagocytosis. SLAMF7 knocked-down in d-THP-1 significantly lowered TLR-induced mRNA expressions of pro-inflammatory markers. SLAM family proteins are differentially regulated by differentiation and TLRs. SLAMF7 enhanced TLR-mediated induction of pro-inflammatory cytokines in monocytes and macrophages but not phagocytosis.
Seasonal changes in physiological and psychological parameters of stress in collegiate swimmers
Authors: Wang Haoyan , Theall Bailey M. , Early Kate S. , Vincellette Cullen , Robelot Lyle , Sharp Rick L. , Marucci Jack , Mullenix Shelly , Calvert Derek , Lemoine Nathan P. , Irving Brain A. , Spielmann Guillaume , Johannsen Neil M.
Published: July 2023

To investigate the seasonal changes in physiological and psychological parameters of stress in collegiate swimmers. Fifteen NCAA Division I swimmers (8 men) participated in a tethered anaerobic swim test to determine physiological responses in an ecologically-relevant, graded exercise test. Wisconsin Upper Respiratory Symptom Survey (WURSS-21), Activation-Deactivation Adjective Check List (AD-ACL), Daily Analysis of Life Demands of Athletes (DALDA), and Pittsburgh Sleep Quality Index were assessed at post-season in April (V1), the end of off-season in June (V2), and pre-season in October (V3). The percent change was determined from V2–V1 (off-season phase), V3–V2 (pre-season phase), V1–V3 (in-season phase). Spearman’s rho correlation was used to examine associations between change in physiological and psychological outcomes. All data results showed a better swim performance occurred at V2. Men tended to have faster speed (p = 0.07) in fewer strokes (p = 0.10) and greater work per stroke (p = 0.10) at V2 than V1. Women were faster during V2 compared to V1 (p = 0.02) and V3 (p = 0.05). Women had fewer strokes (p = 0.02) and greater work per stroke (p = 0.01) at V2 compared to V3. Women had the lowest HR and lactate concentration at V3 compared to other visits (p < 0.05). During the in-season phase, swim speed decreased the greatest extent and stress sources and symptoms assessed by DALDA had greatest elevation (p < 0.05). An increased in stress sources and symptoms assessed by DALDA was associated with an increase in upper respiratory illness from WURSS-21 (rho = 0.44, p = 0.009), being less energetic (rho = − 0.35, p = 0.04) and greater tension state (rho = 0.49, p = 0.003; AD-ACL), and a decrease in swim speed (rho =− 0.38, p = 0.03). Swim performance peaked at off-season when psychological stress was at its lowest. The relationship between DALDA scores with psychological parameters and swim performance suggested physiological and psychological parameters of stress is an important aspect to avoid overtraining when approaching high swim performance.
Metagenomic surveillance for bacterial tick-borne pathogens using nanopore adaptive sampling
Authors: Kipp Evan J. , Lindsey Laramie L. , Khoo Benedict , Faulk Christopher , Oliver Jonathan D. , Larsen Peter A.
Published: July 2023

Technological and computational advancements in the fields of genomics and bioinformatics are providing exciting new opportunities for pathogen discovery and genomic surveillance. In particular, single-molecule nucleotide sequence data originating from Oxford Nanopore Technologies (ONT) sequencing platforms can be bioinformatically leveraged, in real-time, for enhanced biosurveillance of a vast array of zoonoses. The recently released nanopore adaptive sampling (NAS) strategy facilitates immediate mapping of individual nucleotide molecules to a given reference as each molecule is being sequenced. User-defined thresholds then allow for the retention or rejection of specific molecules, informed by the real-time reference mapping results, as they are physically passing through a given sequencing nanopore. Here, we show how NAS can be used to selectively sequence DNA of multiple bacterial tick-borne pathogens circulating in wild populations of the blacklegged tick vector, Ixodes scapularis.
Novel methods of immunogenic antigen selection for serological diagnosis of Parelaphostrongylus tenuis infection
Authors: Richards Jessie , Kania Stephen , Wilson Abigail , Kent Emily , Gerhold Richard
Published: July 2023

This paper outlines methods used to identify novel antigens for use in the development of serological assays. Specifically, we applied these methods to a neurogenic parasitic nematode of cervids called Parelaphostrongylus tenuis. This parasite is of particular concern in both wild and domestic ungulates as it causes significant neurological signs and definitive diagnosis is only possible post-mortem, necessitating the development of serologic assays for antemortem diagnosis. Proteins extracted from P. tenuis organisms were affinity isolated using antibodies enriched from seropositive moose (Alces alces). The proteins were analyzed using mass spectrometry and liquid chromatography to obtain amino acid sequences that were then cross-referenced to open reading frames predicted from an assembled transcriptome. An antigen of interest was assessed for immunogenic epitopes and subsequently synthesized into 10-mer synthetic overlapping peptides representing these regions. These synthetic peptides were then assessed for reactivity against positive and negative moose sera and demonstrated potential use as a serological assay in diagnostic laboratories. Known negative moose sera revealed significantly lower optical density when compared to the positive samples (p < 0.05). This method serves as a pipeline for the construction of diagnostic assays of pathogens in both human and veterinary medicine.
Characterizing endogenous delta oscillations in human MEG
Authors: Gunasekaran Harish , Azizi Leila , van Wassenhove Virginie , Herbst Sophie K.
Published: July 2023

Rhythmic activity in the delta frequency range (0.5–3 Hz) is a prominent feature of brain dynamics. Here, we examined whether spontaneous delta oscillations, as found in invasive recordings in awake animals, can be observed in non-invasive recordings performed in humans with magnetoencephalography (MEG). In humans, delta activity is commonly reported when processing rhythmic sensory inputs, with direct relationships to behaviour. However, rhythmic brain dynamics observed during rhythmic sensory stimulation cannot be interpreted as an endogenous oscillation. To test for endogenous delta oscillations we analysed human MEG data during rest. For comparison, we additionally analysed two conditions in which participants engaged in spontaneous finger tapping and silent counting, arguing that internally rhythmic behaviours could incite an otherwise silent neural oscillator. A novel set of analysis steps allowed us to show narrow spectral peaks in the delta frequency range in rest, and during overt and covert rhythmic activity. Additional analyses in the time domain revealed that only the resting state condition warranted an interpretation of these peaks as endogenously periodic neural dynamics. In sum, this work shows that using advanced signal processing techniques, it is possible to observe endogenous delta oscillations in non-invasive recordings of human brain dynamics.
Transcription and DNA methylation signatures of paternal behavior in hippocampal dentate gyrus of prairie voles
Authors: Waddell Nicholas J. , Liu Yan , Chitaman Javed M. , Kaplan Graham J. , Wang Zuoxin , Feng Jian
Published: July 2023

In socially monogamous prairie voles (Microtus ochrogaster), parental behaviors not only occur in mothers and fathers, but also exist in some virgin males. In contrast, the other virgin males display aggressive behaviors towards conspecific pups. However, little is known about the molecular underpinnings of this behavioral dichotomy, such as gene expression changes and their regulatory mechanisms. To address this, we profiled the transcriptome and DNA methylome of hippocampal dentate gyrus of four prairie vole groups, namely attacker virgin males, parental virgin males, fathers, and mothers. While we found a concordant gene expression pattern between parental virgin males and fathers, the attacker virgin males have a more deviated transcriptome. Moreover, numerous DNA methylation changes were found in pair-wise comparisons among the four groups. We found some DNA methylation changes overlapping with transcription differences, across gene-bodies and promoter regions. Furthermore, the gene expression changes and methylome alterations are selectively enriched in certain biological pathways, such as Wnt signaling, which suggest a canonical transcription regulatory role of DNA methylation in paternal behavior. Therefore, our study presents an integrated view of prairie vole dentate gyrus transcriptome and epigenome that provides a DNA epigenetic based molecular insight of paternal behavior.
Hyperglycemia is associated with duodenal dysbiosis and altered duodenal microenvironment
Authors: Darra Aarti , Singh Vandana , Jena Anuraag , Popli Priyanka , Nada Ritambhra , Gupta Pankaj , Bhadada Sanjay Kumar , Singh Anupam Kumar , Sharma Vishal , Bhattacharya Anish , Agrawal Anurag , Dutta Usha
Published: July 2023

The gut microbiome influences the pathogenesis and course of metabolic disorders such as diabetes. While it is likely that duodenal mucosa associated microbiota contributes to the genesis and progression of increased blood sugar, including the pre-diabetic stage, it is much less studied than stool. We investigated paired stool and duodenal microbiota in subjects with hyperglycemia (HbA1c ≥ 5.7% and fasting plasma glucose > 100 mg/dl) compared to normoglycemic. We found patients with hyperglycemia (n = 33) had higher duodenal bacterial count (p = 0.008), increased pathobionts and reduction in beneficial flora compared to normoglycemic (n = 21). The microenvironment of duodenum was assessed by measuring oxygen saturation using T-Stat, serum inflammatory markers and zonulin for gut permeability. We observed that bacterial overload was correlated with increased serum zonulin (p = 0.061) and higher TNF-α (p = 0.054). Moreover, reduced oxygen saturation (p = 0.021) and a systemic proinflammatory state [increased total leukocyte count (p = 0.031) and reduced IL-10 (p = 0.015)] characterized the duodenum of hyperglycemic. Unlike stool flora, the variability in duodenal bacterial profile was associated with glycemic status and was predicted by bioinformatic analysis to adversely affect nutrient metabolism. Our findings offer new understanding of the compositional changes in the small intestine bacteria by identifying duodenal dysbiosis and altered local metabolism as potentially early events in hyperglycemia.
Implementing IPM in crop management simultaneously improves the health of managed bees and enhances the diversity of wild pollinator communities
Authors: Pecenka Jacob R. , Ingwell Laura L. , Krupke Christian H. , Kaplan Ian
Published: July 2023

Impacts of insecticide use on the health of wild and managed pollinators have been difficult to accurately quantify in the field. Existing designs tend to focus on single crops, even though highly mobile bees routinely forage across crop boundaries. We created fields of pollinator-dependent watermelon surrounded by corn, regionally important crops in the Midwestern US. These fields were paired at multiple sites in 2017–2020 with the only difference being pest management regimes: a standard set of conventional management (CM) practices vs. an integrated pest management (IPM) system that uses scouting and pest thresholds to determine if/when insecticides are used. Between these two systems we compared the performance (e.g., growth, survival) of managed pollinators—honey bees (Apis mellifera), bumble bees (Bombus impatiens)—along with the abundance and diversity of wild pollinators. Compared to CM fields, IPM led to higher growth and lower mortality of managed bees, while also increasing the abundance (+ 147%) and richness (+ 128%) of wild pollinator species, and lower concentrations of neonicotinoids in the hive material of both managed bees. By replicating realistic changes to pest management, this experiment provides one of the first demonstrations whereby tangible improvements to pollinator health and crop visitation result from IPM implementation in agriculture.
Experimental study on basic engineering properties of loess improved by burnt rock
Authors: Chen Kai , Shao Dan , Liu Zhiqi , Chen Lifeng , He Genyi
Published: July 2023

Modifying the loess foundation effectively solved the deformation and settlement of the building foundation and improved its stability. However, burnt rock-solid waste was often used as filling material and light aggregate, while there were few studies on the engineering mechanical properties of modified soil. This paper proposed a method of burnt rock solid waste-modified loess. Therefore, we conducted compression-consolidation and direct shear tests on burnt rock solid waste-modified loess under different burnt rock contents to explore its improved loess’s deformation and strength characteristics. Then, we used an SEM to investigate the modified loess’s micro-structures under different burnt rock contents. The results showed that as the burnt rock-solid waste particle content continued to increase, the void ratio and compressibility coefficient of the samples with different ranges of burnt rock-solid waste particles gradually decreased with rising vertical pressure, while the compressive modulus increased first, then reduced and then increased with the increase of vertical pressure; the shear strength indexes all showed an increasing trend with the increased content of burnt rock-solid waste particles; when the content of burnt rock-solid waste particles was 50%, the compressibility of mixed soil was the lowest, the shear strength was the largest, and the compaction effect and shear resistance were the best. However, when the content of burnt rock particles was 10–20%, the shear strength of the soil improved significantly within the content range. The mechanism of burnt rock-solid waste to enhance the strength of the loess structure was mainly to reduce the porosity and average area of soil, significantly improve the strength and stability of mixed soil particles, and thus significantly improve the mechanical properties of soil. The results of this research will provide technical support for safe engineering construction and geological disaster prevention and control in loess areas.
Short-term impact of diurnal temperature range on cardiovascular diseases mortality in residents in northeast China
Authors: Kai Xuan , Hong Zhimin , Hong Yang , Wang Xiaolei , Li Chunyang
Published: July 2023

It has been reported that cardiovascular disease (CVD) has become one of the major threats to global public health and is associated with climate change. Several previous studies have shown the influence of ambient temperature on CVD, but lack some evidence for the short-term effect of diurnal temperature range (DTR) on CVD mortality in northeast China. This is the first study to assess the correlation between DTR and CVD mortality in Hulunbuir located in northeast China. Daily CVD mortality data and meteorological data were collected from 2014 to 2020. A quasi-Poisson generalized linear regression with a distributed lag non-linear model (DLNM) was applied to exploring the short-term impact of DTR on CVD mortality. Stratified analyses by gender, age, and season were conducted and the short-term impacts of extremely high DTR on CVD mortality were investigated. In this study, a total of 21,067 CVD mortality cases were recorded in Hulunbuir, China from 2014 to 2020. Compared to the reference value (11.20 $$^{\circ }$$

∘

C, 50$${\text{ th }}$$

th

percentile), a “U-shaped” non-linear relationship between DTR and CVD mortality was observed, and extremely high DTR increased the risk of CVD mortality. The short-term effect of extremely high DTR occurred immediately and lasted up to 6 days. In addition, the male and the age $$\ge$$
≥
65 groups were more likely to be affected by extremely high DTR compared with the female and the age < 65 groups, respectively. The results also showed that extremely high DTR in cold season had a more adverse effect on CVD mortality than warm season. This study suggests that extremely high DTR for cold season should be paid enough attention to for residents in northeast China. The male and the age $$\ge$$
≥
65 groups were more vulnerable to the impacts of DTR. The study results may provide some suggestions for decision-making by local public health authorities to avoid the adverse impacts of high DTR, and improve the health of residents, especially vulnerable groups in cold season.