bluuebunny/crossref_metadata_2025 huggingface dataset:
Application of the Fractal Method to the Characterization of Organic Heterogeneities in Shales and Exploration Evaluation of Shale Oil
Authors: Liu Bo , Yao Liangwen , Fu Xiaofei , He Bo , Bai Longhui
Published: March 2019

The first member of the Qingshankou Formation, in the Gulong Sag in the northern part of the Songliao Basin, has become an important target for unconventional hydrocarbon exploration. The organic-rich shale within this formation not only provides favorable hydrocarbon source rocks for conventional reservoirs, but also has excellent potential for shale oil exploration due to its thickness, abundant organic matter, the overall mature oil generation state, high hydrocarbon retention, and commonly existing overpressure. Geochemical analyses of the total organic carbon content (TOC) and rock pyrolysis evaluation (Rock-Eval) have allowed for the quantitative evaluation of the organic matter in the shale. However, the organic matter exhibits a highly heterogeneous spatial distribution and its magnitude varies even at the millimeter scale. In addition, quantification of the TOC distribution is significant to the evaluation of shale reservoirs and the estimation of shale oil resources. In this study, well log data was calibrated using the measured TOC of core samples collected from 11 boreholes in the study area; the continuous TOC distribution within the target zone was obtained using the △logR method; the organic heterogeneity of the shale was characterized using multiple fractal models, including the box-counting dimension (Bd), the power law, and the Hurst exponent models. According to the fractal dimension (D) calculation, the vertical distribution of the TOC was extremely homogeneous. The power law calculation indicates that the vertical distribution of the TOC in the first member of the Qingshankou Formation is multi-fractal and highly heterogeneous. The Hurst exponent varies between 0.23 and 0.49. The lower values indicate higher continuity and enrichment of organic matter, while the higher values suggest a more heterogeneous organic matter distribution. Using the average TOC, coefficient of variation (CV), Bd, D, inflection point, and the Hurst exponent as independent variables, the interpolation prediction method was used to evaluate the exploration potential of the study area. The results indicate that the areas containing boreholes B, C, D, F, and I in the western part of the Gulong Sag are the most promising potential exploration areas. In conclusion, the findings of this study are of significant value in predicting favorable exploration zones for unconventional reservoirs.
UUV Simulation Modeling and its Control Method: Simulation and Experimental Studies
Authors: Li Ji-Hong , Kim Min-Gyu , Kang Hyungjoo , Lee Mun-Jik , Cho Gun Rae
Published: March 2019

This paper presents the development of an unmanned underwater vehicle (UUV) platform, especially the derivation of the vehicle’s simulation model and its control method to overcome strong sea current. The platform is designed to have a flattened ellipsoidal exterior so as to minimize the hydrodynamic damping on the horizontal plane. Four horizontal thrusters with the identical specifications are symmetrically mounted on the horizontal plane, and each of them has the same thrust dynamics in both forward and reverse directions. In addition, there are three vertical thrusters used to handle the vehicle’s roll, pitch and heave motions. Control strategy proposed in this paper to overcome strong current is that: maximizing the vectored horizontal thrust force against the sea current without or with the least of the vehicle’s rotation on the horizontal plane. For the vehicle model, due to it being symmetric in all of three axes, the vehicle dynamics can be simplified and all of hydrodynamic coefficients are calculated through both of theoretical and empirically-derived formulas. Numerical simulations and experimental studies in both of the water tank and the circulating water channel are carried out to demonstrate the vehicle’s capability of overcoming strong current.
Valuing Our National Parks: An Ecological Economics Perspective
Authors: Sutton Paul C. , Duncan Sophia L. , Anderson Sharolyn J.
Published: March 2019

The annual budget for the United States National Park Service was roughly $3 billion in 2016. This is distributed amongst 405 National Parks, 23 national scenic and historic trails, and 60 wild and scenic rivers. Entrance fees and concessions generate millions of dollars in income for the National Park Service; however, this metric fails to account for the total value of the National Parks. In failing to consider the value of the ecosystem services provided by the National Parks, we fail to quantify and appreciate the contributions our parks make to society. This oversight allows us to continue to underfund a valuable part of our natural capital and consequently damage our supporting environment, national heritage, monetary economy, and many of our diverse cultures. We explore a simple benefits transfer valuation of the United States’ national parks using National Land Cover Data from 2011 and ecosystem service values determined by Costanza et al. This produces an estimate suggesting the parks provide $98 billion/year in ecosystem service value. If the natural infrastructure ‘asset’ that is our national park system had a budget comparable to a piece of commercial real estate of this value, the annual budget of the National Park Service would be roughly an order of magnitude larger at something closer to $30 billion rather than $3 billion.
Expansion of Oil Palm Plantations in Indonesia’s Frontier: Problems of Externalities and the Future of Local and Indigenous Communities
Authors: Andrianto Agus , Komarudin Heru , Pacheco Pablo
Published: March 2019

The expansion of oil palm plantations in Papua province, Indonesia, involves the conversion of forests, among other land types in the landscapes, which are a source of clan members’ livelihoods. The way in which this expansion occurs makes it necessary to understand the factors associated with why companies look for frontier lands and what externalities are generated during both the land acquisition and plantation development periods. Using a spatial analysis of the concession areas, along with data from household surveys of each clan from the Auyu, Mandobo, and Marind tribes who release land to companies, we find that investors are motivated to profit from timber harvested from the clearing of lands for plantations, activity that is facilitated by the local government. Land acquisition and plantation development have resulted in externalities to indigenous landowners in the form of time and money lost in a series of meetings and consultations involving clan members and traditional elders. Other externalities include the reduced welfare of people due to loss of livelihoods, and impacts on food security.
Sound Symbolism Facilitates Long-Term Retention of the Semantic Representation of Novel Verbs in Three-Year-Olds
Authors: Kantartzis Katerina , Imai Mutsumi , Evans Danielle , Kita Sotaro
Published: March 2019

Previous research has shown that sound symbolism facilitates action label learning when the test trial used to assess learning immediately followed the training trial in which the (novel) verb was taught. The current study investigated whether sound symbolism benefits verb learning in the long term. Forty-nine children were taught either sound-symbolically matching or mismatching pairs made up of a novel verb and an action video. The following day, the children were asked whether a verb can be used for a scene shown in a video. They were tested with four videos for each word they had been taught. The four videos differed as to whether they contained the same or different actions and actors as in the training video: (1) same-action, same-actor; (2) same-action, different-actor; (3) different-action, same-actor; and (4) different-action, different-actor. The results showed that sound symbolism significantly improved the childrens’ ability to encode the semantic representation of the novel verb and correctly generalise it to a new event the following day. A control experiment ruled out the possibility that children were generalising to the “same-action, different-actor” video because they did not recognize the actor change due to the memory decay. Nineteen children were presented with the stimulus videos that had also been shown to children in the sound symbolic match condition in Experiment 1, but this time the videos were not labeled. In the test session the following day, the experimenter tested the children’s recognition memory for the videos. The results indicated that the children could detect the actor change from the original training video a day later. The results of the main experiment and the control experiment support the idea that a motivated (iconic) link between form and meaning facilitates the symbolic development in children. The current study, along with recent related studies, provided further evidence for an iconic advantage in symbol development in the domain of verb learning. A motivated form-meaning relationship can help children learn new words and store them long term in the mental lexicon.
Application of Nanoindentation and 2D and 3D Imaging to Characterise Selected Features of the Internal Microstructure of Spun Concrete
Authors: Michałek Jarosław , Pachnicz Michał , Sobótka Maciej
Published: March 2019

The spinning of concrete is a process in which concrete mixture is moulded and compacted under the action of the centrifugal force arising during the fast rotational motion of the mould around its longitudinal axis. As a result of the spinning of the liquid concrete mixture, an element annular in cross section, characterised by an inhomogeneous layered wall structure, is produced. The heavier constituents tend towards the cross-section wall’s outer side, while the lighter components tend towards its inner side. The way in which the particular constituents are distributed in the element’s cross section is of key importance for the macro properties of the manufactured product. This paper presents procedures for investigating spun concrete and interpreting the results of such investigations, which make it possible to characterise the microstructure of the concrete. Three investigative methods were used to assess the distribution of the constituents in the cross section of the element: micro-computed tomography (µCT), 2D imaging (using an optical scanner) and nanoindentation. A procedure for interpreting and analysing the results is proposed. The procedure enables one to quantitatively characterise the following features of the microstructure of spun concrete: the mechanical parameters of the mortar, the aggregate content, the pore content, the cement paste content, the aggregate grading and the size (dimensions) of the pores. Special attention is devoted to the determination of the variation of the analysed quantities in the cross section of the element. The result of the application of the investigative procedures is presented for an exemplary spun concrete element. The proposed procedures constitute a valuable tool for evaluating the process of manufacturing spun concrete elements.
Conductance Changes in Bovine Serum Albumin Caused by Drug-Binding Triggered Structural Transitions
Authors: Yu Jing , Chen Yun , Xiong Liqun , Zhang Xiaoyue , Zheng Yue
Published: March 2019

Proteins, due to their binding selectivity, are promising candidates for fabricating nanoscale bio-sensors. However, the influence of structural change on protein conductance caused by specific protein-ligand interactions and disease-induced degeneration still remains unknown. Here, we excavated the relationship between circular dichroism (CD) spectroscopy and conductive atomic force microscopy (CAFM) to reveal the effect of the protein secondary structures changes on conductance. The secondary structure of bovine serum albumin (BSA) was altered by the binding of drugs, like amoxicillin (Amox), cephalexin (Cefa), and azithromycin (Azit). The CD spectroscopy shows that the α-helical and β-sheet content of BSA, which varied according to the molar ratio between the drug and BSA, changed by up to 6%. The conductance of BSA monolayers in varying drug concentrations was further characterized via CAFM. We found that BSA conductance has a monotonic relation with α-helical content. Moreover, BSA conductance seems to be in connection with the binding ability of drugs and proteins. This work elucidates that protein conductance variations caused by secondary structure transitions are triggered by drug-binding and indicate that electrical methods are of potential application in protein secondary structure analysis.
Investigation of Shear-Induced Deformation of Reinforcing Textiles by Optical Measurement Devices
Authors: Rothe Stefan , Wendt Ellen , Krzywinski Sybille , Halász Marianna , Bakonyi Peter , Tamás Peter , Bojtos Attila
Published: March 2019

When fiber-reinforced plastic (FRP) components are designed, it is very important to ensure that textiles are formed into complex 3D geometries without folds, and that the reinforcing structure is oriented appropriately. Most research in this context is focused on finite element (FE) forming simulations and the required characterization of textile reinforcements. However, the early stage of the design of FRPs, where kinematic draping simulations are used, is barely considered. In particular, the need for a critical shear angle for the execution and evaluation of kinematic draping simulations is often neglected. This paper presents an extended picture frame test stand with an optical device recording shear-induced deformations with the help of a laser line emitter. Associated hardware and software for detecting and quantifying the fold formation during a picture frame test were developed. With the additional recorded information, a material-specific critical shear angle can be determined, material behaviors can be compared, and FE-based simulation methods can be evaluated. This innovative test stand and the associated software tools will help engineers to decide on suitable materials and improve transparency in the early stages of the design process.
Techno-Economic Assessment of a Scaled-Up Meat Waste Biorefinery System: A Simulation Study
Authors: Okoro Oseweuba Valentine , Sun Zhifa , Birch John
Published: March 2019

While exports from the meat industry in New Zealand constitute a valuable source of foreign exchange, the meat industry is also responsible for the generation of large masses of waste streams. These meat processing waste streams are largely biologically unstable and are capable of leading to unfavourable environmental outcomes if not properly managed. To enable the effective management of the meat processing waste streams, a value-recovery based strategy, for the complete valorisation of the meat processing waste biomass, is proposed. In the present study therefore, a biorefinery system that integrates the biomass conversion technologies of hydrolysis, esterification, anaerobic digestion and hydrothermal liquefaction has been modelled, simulated and optimized for enhanced environmental performance and economic performance. It was determined that an initial positive correlation between the mass feed rate of the waste to the biorefinery system and its environmental performance exists. However, beyond an optimal total mass feed rate of the waste stream there is a deterioration of the environmental performance of the biorefinery system. It was also determined that economies of scale ensure that any improvement in the economic performance of the biorefinery system with increasing total mass feed rate of the waste stream, is sustained. The present study established that the optimized meat waste biorefinery system facilitated a reduction in the unit production costs of the value-added products of biodiesel, biochar and biocrude compared the literature-obtained unit production costs of the respective aforementioned products when generated from stand-alone systems. The unit production cost of biogas was however shown to be comparable to the literature-obtained unit production cost of biogas. Finally, the present study showed that the optimized meat processing waste biorefinery could achieve enhanced economic performance while simultaneously maintaining favourable environmental sustainability.
Pharmacotherapy Literacy and Parental Practice in Use of Over-the-Counter Pediatric Medicines
Authors: Krajnović Dušanka , Ubavić Stana , Bogavac-Stanojević Nataša
Published: March 2019

Background and objectives: Pharmaceutical literacy skills of parents are crucial for appropriate and safe medication use in pre-school children (ages 1–7 years). A recent study on pharmacotherapy literacy from Serbia showed that one in five parents have difficulty understanding common information about the use of medicines. Because antipyretics are considered to be the most frequently used group of over-the-counter (OTC) medications during the pre-school period, we aimed to: (i) examine parental practice and expectations in antipyretic medication use, and (ii) analyze associations of parental practice and expectations related to socio-economic status and pharmacotherapy literacy. Materials and methods: A cross-sectional survey using a self- report validated specific instrument was conducted with the parents of pre-school children in kindergartens in Belgrade, Serbia. Pharmacotherapy literacy refers to the knowledge and personal skills needed to meet the complex demands of medicine use in both healthcare and non-healthcare settings. A comprehensive literature review, expert-focus group consultation, and pre-testing were employed in 4-item multiple-choice test development to explore practice and expectations related to the use of OTC pediatric antipyretic medicines. Results: The final analytical cohort was comprised of 813 participants, the majority (63.3%) chose a medicine based on a physician’s suggestion and only 15.4% of parents reported they would follow the advice of a pharmacist. More than a half of parents (54.1%) would need advice about antipyretic medicine from a pharmacist, firstly in a simpler language. Parents satisfied with the information given by a pharmacist had higher pharmacotherapy literacy, compared to parents with lower levels (OR–0.718, 95%CI (0.597–0.865), p < 0.001). Men had a higher expectation of pharmacists to explain medicine use in a simpler language (OR–1.630, 95%CI (1.063–2.501), p = 0.025), as well as parents with three or more children (OR–2.527, 95%CI (1.43–4.459), p = 0.001). Parents with higher knowledge about medicine use were less likely to ask for simpler information (OR–0,707; 95%CI (0,583–0,856), p < 0,001). Conclusions: Our main finding is that practice in antipyretic OTC medicine use was associated with levels of parental pharmacotherapy literacy. The expectations of pharmacists were higher among parents with lower levels of pharmacotherapy literacy, who expected more information in a simpler and more precise language. This study highlighted the need for pharmacists to identify risks in parental practice and to provide information about medicines to parents of pre-school children in a simpler and more appropriate way.
Nanoemulsions of Essential Oils: New Tool for Control of Vector-Borne Diseases and In Vitro Effects on Some Parasitic Agents
Authors: Echeverría Javier , Albuquerque Ricardo
Published: March 2019

The control of infectious/parasitic diseases is a continuing challenge for global health, which in turn requires new methods of action and the development of innovative agents to be used in its prevention and/or treatment. In this context, the control of vectors and intermediate hosts of etiological agents is an efficient method in the prevention of human and veterinary diseases. In later stages, it is necessary to have bioactive compounds that act efficiently on the agents that produce the disease. However, several synthetic agents have strong residual effects in humans and other animals and cause environmental toxicity, affecting fauna, flora and unbalancing the local ecosystem. Many studies have reported the dual activity of the essential oils (EOs): (i) control of vectors that are important in the cycle of disease transmission, and (ii) relevant activity against pathogens. In general, EOs have an easier degradation and cause less extension of environmental contamination. However, problems related to solubility and stability lead to the development of efficient vehicles for formulations containing EOs, such as nanoemulsions. Therefore, this systematic review describes several studies performed with nanoemulsions as carriers of EOs that have larvicidal, insecticidal, repellent, acaricidal and antiparasitic activities, and thus can be considered as alternatives in the vector control of infectious and parasitic diseases, as well as in the combat against etiological agents of parasitic origin.
Recovery of Lignosulfonates from Spent Sulfite Liquor Using Ceramic Hollow-Fiber Membranes
Authors: Humpert Daniel , Ebrahimi Mehrdad , Stroh Annika , Czermak Peter
Published: March 2019

Spent sulfite liquor is an abundant but currently less used wastewater stream from the pulp and paper industry. The recovery of lignin from this resource would provide an inexpensive raw material for the manufacture of fuels and fine chemicals. Here we investigated the suitability of ceramic hollow-fiber membranes for the concentration of spent sulfite liquor as an alternative to common membrane technologies. We tested three ceramic hollow-fiber membranes (3, 8, and 30 nm) in different membrane processes (fed-batch and total recycle mode) and compared their performance with the widely-used tubular membrane geometry. We also evaluated backflushing as a strategy to reduce membrane fouling during filtration. The juxtaposition of the two membrane geometries revealed that wall shear stress is the most important process parameter for the assessment of membrane performance according to permeate flux. The higher the wall shear stress, the higher the permeate flux. Due to the smaller inner diameter of the hollow-fiber membranes, higher wall shear stress can be achieved more easily. Backflushing had no effect on the permeate flux during the concentration experiments.
Oxidation of Thiosulfate with Oxygen Using Copper (II) as a Catalyst
Authors: González Lara Juan Manuel , Cardona Francisco Patiño , Vallmajor Antonio Roca , Cadevall Montserrat Cruells
Published: March 2019

Thiosulfate effluents are generated in the photography and radiography industrial sectors, and in a plant in which thiosulfates are used to recover the gold and silver contained in ores. Similar effluents also containing thiosulfate are those generated from the petrochemical, pharmaceutical and pigment sectors. In the future, the amounts of these effluents may increase, particularly if the cyanides used in the extraction of gold and silver from ores are substituted by thiosulfates, or if the same happens to electronic scrap or in metallic coating processes. This paper reports a study of the oxidation of thiosulfate, with oxygen using copper (II) as a catalyst, at a pH between 4 and 5. The basic idea is to avoid the formation of tetrathionate and polythionate, transforming the thiosulfate into sulfate. The nature of the reaction and a kinetic study of thiosulfate transformation, by reaction with oxygen and Cu2+ at a ppm level, are determined and reported. The best conditions were obtained at 60 °C, pH 5, with an initial concentration of copper of 53 ppm and an oxygen pressure of 1 atm. Under these conditions, the thiosulfate concentration was reduced from 1 g·L−1 to less than 20 ppm in less than three hours.
Corrosion Study of Implanted TiN Electrodes Using Excessive Electrical Stimulation in Minipigs
Authors: Meijs Suzan , Rechendorff Kristian , Sørensen Søren , Rijkhoff Nico J.M.
Published: March 2019

(1) Background: Titanium nitride (TiN) electrodes have been used for implantable stimulation and sensing electrodes for decades. Nevertheless, there still is a discrepancy between the in vitro and in vivo determined safe charge injection limits. This study investigated the consequences of pulsing implanted electrodes beyond the in vivo safe charge injection limits. (2) Methods: The electrodes were implanted for a month and then pulsed at 20 mA and 50 mA and 200 Hz and 400 Hz. Afterwards, the electrodes were investigated using electrochemical and analytical methods to evaluate whether electrode degradation had occurred. (3) Results: Electrochemical tests showed that electrodes that pulsed at 20 mA and 200 Hz (lowest electrical dose) had a significantly lower charge injection capacity and higher impedance than the other used and unused electrodes. (4) Conclusions: The electrodes pulsed at the lowest electrical dose, for which no tissue damage was found, appeared to have degraded. Electrodes pulsed at higher electrical doses for which tissue damage did occur, on the other hand, show no significant degradation in electrochemical tests compared to unused implanted and not implanted electrodes. It is thus clear that the tissue surrounding the electrode has an influence on the charge injection properties of the electrodes and vice versa.
Computational Methods for Lifetime Prediction of Metallic Components under High-Temperature Fatigue
Authors: Kindrachuk Vitaliy , Fedelich Bernard , Rehmer Birgit , Peter Frauke
Published: March 2019

The issue of service life prediction of hot metallic components subjected to cyclic loadings is addressed. Two classes of lifetime models are considered, namely, the incremental lifetime rules and the parametric models governed by the fracture mechanics concept. Examples of application to an austenitic cast iron are presented. In addition, computational techniques to accelerate the time integration of the incremental models throughout the fatigue loading history are discussed. They efficiently solve problems where a stabilized response of a component is not observed, for example due to the plastic strain which is no longer completely reversed and accumulates throughout the fatigue history. The performance of such an accelerated integration technique is demonstrated for a finite element simulation of a viscoplastic solid under repeating loading–unloading cycles.
Creation of Artificial Cell-Like Structures Promoted by Microfluidics Technologies
Authors: Sato Yusuke , Takinoue Masahiro
Published: March 2019

The creation of artificial cells is an immensely challenging task in science. Artificial cells contribute to revealing the mechanisms of biological systems and deepening our understanding of them. The progress of versatile biological research fields has clarified many biological phenomena, and various artificial cell models have been proposed in these fields. Microfluidics provides useful technologies for the study of artificial cells because it allows the fabrication of cell-like compartments, including water-in-oil emulsions and giant unilamellar vesicles. Furthermore, microfluidics also allows the mimicry of cellular functions with chip devices based on sophisticated chamber design. In this review, we describe contributions of microfluidics to the study of artificial cells. Although typical microfluidic methods are useful for the creation of artificial-cell compartments, recent methods provide further benefits, including low-cost fabrication and a reduction of the sample volume. Microfluidics also allows us to create multi-compartments, compartments with artificial organelles, and on-chip artificial cells. We discuss these topics and the future perspective of microfluidics for the study of artificial cells and molecular robotics.
Effect of Injection Speed on Oocyte Deformation in ICSI
Authors: Hajiyavand Amir , Saadat Mozafar , Abena Alessandro , Sadak Ferhat , Sun Xiaochen
Published: March 2019

Oocyte deformation during injection is a major cause of potential cell damage which can lead to failure in the Intracytoplasmic Sperm Injection (ICSI) operation used as an infertility treatment. Injection speed plays an important role in the deformation creation. In this paper the effect of different speeds on deformation of zebrafish embryos is studied using a specially designed experimental set-up. An analytical model is developed in order to link injection force, deformation, and injection speed. A finite element (FE) model is also developed to analyse the effect of injection speed, allowing the production of additional information that is difficult to obtain experimentally, e.g., deformation and stress fields on the oocyte. The numerical model is validated against experimental results. Experimental results indicate that by increasing the injection speed, the deformation decreases. However, higher speeds cause higher levels of injection force and force fluctuation, leading to a higher vibration during injection. For this reason, an optimum injection speed range is determined. Finally, the FE model was validated against experimental results. The FE model is able to predict the force-deformation variation during injection for different speeds. This proves to be useful for future studies investigating different injection conditions.
Confirmation of Interlayer Sulfidization of Malachite by TOF-SIMS and Principal Component Analysis
Authors: Deng Jiushuai , Lai Hao , Wen Shuming , Li Shimei
Published: March 2019

In this work, a sulfidization mechanism of malachite was confirmed based on the depth profile product, principal component, and depth profile curve analyses of time-of-flight secondary ion mass spectrometry (TOF-SIMS). The results showed that Cu/S species, including fragment ion peaks of Cu2S+, Cu3S+, S−, HS−, S2−, CuS2−, and CuS3−, were present in the inner layers of sulfidized malachite in the positive and negative spectral ranges 75–400 and 30–470 m/z. Na2S reacted with the surface and inner atoms, causing simultaneous sulfidization of malachite on the surface and in the inner layers. The inner layer mainly contained positive fragment ions with large Cu/S ratios. In summary, the interlayer sulfidization phenomenon was confirmed and the differences in sulfidization products between the surface and inner layers were determined.
The Effects of Ergosta-7,9(11),22-trien-3β-ol from Antrodia camphorata on the Biochemical Profile and Exercise Performance of Mice
Authors: Chen Yi-Ming , Sung Hsin-Ching , Kuo Yueh-Hsiung , Hsu Yi-Ju , Huang Chi-Chang , Liang Hsin-Li
Published: March 2019

Antrodia camphorata (AC) is a rare and unique mushroom that is difficult to cultivate. Previous studies have demonstrated the bioactivity of the compound Ergosta-7,9(11),22-trien-3β-ol (EK100) from AC in submerged culture. The purpose of this study is to evaluate the potential beneficial effects of EK100 on fatigue and ergogenic functions following physiological challenge. Male ICR (Institute of Cancer Research) mice were randomly divided into three groups (n = 8 per group) and orally administered EK100 for six weeks at 0 (Vehicle), 10 (EK100-1X), and 20 (EK100-2X) mg/kg/day. The six-week Ek100 supplementation significantly increased grip strength (p = 0.0051) in trend analysis. Anti-fatigue activity was evaluated using 15-min. acute exercise testing and measuring the levels of serum lactate, ammonia, glucose, blood urea nitrogen (BUN), and creatine kinase (CK) after a 15-min. swimming exercise. Our results indicate that AC supplementation leads to a dose-dependent decrease in serum lactate, ammonia, BUN, and CK activity after exercise and significantly increases serum glucose and glycogen content in liver tissues. Biochemical and histopathological data demonstrated that long term daily administration of EK100 for over six weeks (subacute toxicity) was safe. EK100’s anti-fatigue properties appear to be through the preservation of energy storage, increasing blood glucose and liver glycogen content, and decreasing the serum levels of lactate, ammonia, BUN, and CK. EK100 could potentially be used to improve exercise physiological adaptation, promote health, and as a potential ergogenic aid in combination with different nutrient strategies.
Hybrid Interface in Sepiolite Rubber Nanocomposites: Role of Self-Assembled Nanostructure in Controlling Dissipative Phenomena
Authors: Cobani Elkid , Tagliaro Irene , Geppi Marco , Giannini Luca , Leclère Philippe , Martini Francesca , Nguyen Thai Cuong , Lazzaroni Roberto , Scotti Roberto , Tadiello Luciano , Di Credico Barbara
Published: March 2019

Sepiolite (Sep)–styrene butadiene rubber (SBR) nanocomposites were prepared by using nano-sized sepiolite (NS-SepS9) fibers, obtained by applying a controlled surface acid treatment, also in the presence of a silane coupling agent (NS-SilSepS9). Sep/SBR nanocomposites were used as a model to study the influence of the modified sepiolite filler on the formation of immobilized rubber at the clay-rubber interface and the role of a self-assembled nanostructure in tuning the mechanical properties. A detailed investigation at the macro and nanoscale of such self-assembled structures was performed in terms of the organization and networking of Sep fibers in the rubber matrix, the nature of both the filler–filler and filler–rubber interactions, and the impact of these features on the reduced dissipative phenomena. An integrated multi-technique approach, based on dynamic measurements, nuclear magnetic resonance analysis, and morphological investigation, assessed that the macroscopic mechanical properties of clay nanocomposites can be remarkably enhanced by self-assembled filler structures, whose formation can be favored by manipulating the chemistry at the hybrid interfaces between the clay particles and the polymers.
1-Laurin-3-Palmitin as a Novel Matrix of Solid Lipid Particles: Higher Loading Capacity of Thymol and Better Stability of Dispersions Than Those of Glyceryl Monostearate and Glyceryl Tripalmitate
Authors: Shi Hao , Huang Shuangshuang , He Junbo , Han Lijuan , Zhang Weinong , Zhong Qixin
Published: March 2019

To develop solid lipid nanoparticles (SLNs) with a new lipid matrix for delivery of hydrophobic bioactive molecules, high purity 1-laurin-3-palmitin (1,3-LP) was synthesized and the prepared 1,3-LP SLNs were compared with those of two common SLN matrices in glyceryl monostearate (GMS) and glyceryl tripalmitate (PPP). Conditions of preparing SLNs were first optimized by evaluating the particle size, polydispersity index (PDI), zeta-potential, and stability. Thereafter, the performance of SLN loading of a model compound in thymol was studied. The loading capacity of thymol in 1,3-LP SLNs was 16% of lipids and higher than 4% and 12% for GMS- and PPP-SLNs, respectively. The 1,3-LP SLNs also had the best efficiency to entrapment thymol during the prolonged storage. X-ray diffraction (XRD) analyses confirmed the excellent crystalline stability of 1,3-LP leading to the stable entrapment efficiency and better stability of thymol-loaded SLNs. Conversely, the polymorphic transformation of GMS and PPP resulted in the declined entrapment efficiency of thymol in the corresponding SLNs. This work indicated the 1,3-diacylglycerol (DAG) SLNs could be used as a promising delivery system for the encapsulation of hydrophobic bioactive molecules with high loading capacity and stability.
Structural and Functional Stability of DNA Nanopores in Biological Media
Authors: Burns Jonathan R. , Howorka Stefan
Published: March 2019

DNA nanopores offer a unique nano-scale foothold at the membrane interface that can help advance the life sciences as biophysical research tools or gate-keepers for drug delivery. Biological applications require sufficient physiological stability and membrane activity for viable biological action. In this report, we determine essential parameters for efficient nanopore folding and membrane binding in biocompatible cell media. The parameters are identified for an archetypal DNA nanopore composed of six interwoven strands carrying cholesterol lipid anchors. Using gel electrophoresis and fluorescence spectroscopy, the nanostructures are found to assemble efficiently in cell media, such as LB and DMEM, and remain structurally stable at physiological temperatures. Furthermore, the pores’ oligomerization state is monitored using fluorescence spectroscopy and confocal microscopy. The pores remain predominately water-soluble over 24 h in all buffer systems, and were able to bind to lipid vesicles after 24 h to confirm membrane activity. However, the addition of fetal bovine serum to DMEM causes a significant reduction in nanopore activity. Serum proteins complex rapidly to the pore, most likely via ionic interactions, to reduce the effective nanopore concentration in solution. Our findings outline crucial conditions for maintaining lipidated DNA nanodevices, structurally and functionally intact in cell media, and pave the way for biological studies in the future.
Dietary Long-Chain Fatty Acids and Cognitive Performance in Older Australian Adults
Authors: MacDonald-Wicks Lesley , McEvoy Mark , Magennis Eliza , Schofield Peter W. , Patterson Amanda J. , Zacharia Karly
Published: March 2019

Convincing evidence exists for the positive effect of an improvement in diet quality on age-related cognitive decline, in part due to dietary fatty acid intake. A cross-sectional analysis of data from the Hunter Community Study (HCS) (n = 2750) was conducted comparing dietary data from a validated Food Frequency Questionnaire (FFQ) with validated cognitive performance measures, Audio Recorded Cognitive Screen (ARCS) and Mini Mental State Examination (MMSE). Adjusted linear regression analysis found statistically significant associations between dietary intake of total n-6 fatty acids (FA), but no other FAs, and better cognitive performance as measured by the ARCS (RC = 0.0043; p = 0.0004; R2 = 0.0084). Multivariate regression analyses of n-6 FA intakes in quartiles showed that, compared with the lowest quartile (179.8–1150.3 mg), those in the highest quartile (2315.0–7449.4 mg) had a total ARCS score 2.1 units greater (RC = 10.60466; p = 0.006; R2 = 0.0081). Furthermore, when n-6 FA intake was tested against each of the ARCS domains, statistically significant associations were observed for the Fluency (RC = 0.0011432; p = 0.007; R2 = 0.0057), Visual (RC = 0.0009889; p = 0.034; R2 = 0.0050), Language (RC = 0.0010651; p = 0.047; R2 = 0.0068) and Attention (RC = 0.0011605; p = 0.017; R2 = 0.0099) domains, yet there was no association with Memory (RC = −0.000064; p = 0.889; R2 = 0.0083). No statistically significant associations were observed between FA intakes and MMSE. A higher intake of total n-6 FA, but not other types of FA, was associated with better cognitive performance among a representative sample of older aged Australian adults.
The Influence of Meal Frequency and Timing on Health in Humans: The Role of Fasting
Authors: Paoli Antonio , Tinsley Grant , Bianco Antonino , Moro Tatiana
Published: March 2019

The influence of meal frequency and timing on health and disease has been a topic of interest for many years. While epidemiological evidence indicates an association between higher meal frequencies and lower disease risk, experimental trials have shown conflicting results. Furthermore, recent prospective research has demonstrated a significant increase in disease risk with a high meal frequency (≥6 meals/day) as compared to a low meal frequency (1–2 meals/day). Apart from meal frequency and timing we also have to consider breakfast consumption and the distribution of daily energy intake, caloric restriction, and night-time eating. A central role in this complex scenario is played by the fasting period length between two meals. The physiological underpinning of these interconnected variables may be through internal circadian clocks, and food consumption that is asynchronous with natural circadian rhythms may exert adverse health effects and increase disease risk. Additionally, alterations in meal frequency and meal timing have the potential to influence energy and macronutrient intake.A regular meal pattern including breakfast consumption, consuming a higher proportion of energy early in the day, reduced meal frequency (i.e., 2–3 meals/day), and regular fasting periods may provide physiological benefits such as reduced inflammation, improved circadian rhythmicity, increased autophagy and stress resistance, and modulation of the gut microbiota
Muscle Antioxidant Enzymes Activity and Gene Expression Are Altered by Diet-Induced Increase in Muscle Essential Fatty Acid (α-linolenic acid) Concentration in Sheep Used as a Model
Authors: Ponnampalam Eric N. , Vahedi Vahid , Giri Khageswor , Lewandowski Paul , Jacobs Joe L. , Dunshea Frank R.
Published: March 2019

This study investigated the effect of dietary manipulations on muscle fatty acid composition, the activities and relative mRNA expressions of antioxidant enzymes and the relationship between muscle enzyme activity or mRNA expression and alpha linolenic acid (ALA) concentration in sheep. Eighty-four lambs blocked on liveweight were randomly allocated to four dietary treatments, lucerne pasture (Lucerne), annual ryegrass pasture (Ryegrass), feedlot pellets (Feedlot) or annual ryegrass plus feedlot pellets (RyeFeedlot). After six weeks of feeding, lambs were slaughtered and within 30 min post-mortem, samples collected from the longissimus lumborum (LL) muscle for RNA isolation and measurement of antioxidant enzyme activities. At 24 h post-mortem, LL samples were collected for determination of fatty acid concentrations. Feedlot treatment decreased ALA, eicosapentaenoic (EPA), docosapentaenoic (DPA) and docosahexaenoic (DHA) concentrations compared with other treatments and increased linoleic acid (LA) and arachidonic acid (AA) compared with Lucerne and Ryegrass (p < 0.001). The activity of Glutathione peroxidase (GPX1, p < 0.001) and Superoxide dismutase (SOD2, p < 0.001) enzymes in the muscle increased with Lucerne compared to other treatments. Lucerne increased muscle gpx1 mRNA expression by 1.74-fold (p = 0.01) and 1.68-fold (p = 0.05) compared with Feedlot and other diets, respectively. The GPX1 (r2 = 0.319, p = 0.002) and SOD2 (r2 = 0.244, p = 0.009) enzyme activities were positively related to ALA. There was a positive linear relationship between muscle gpx1 (r2 = 0.102, p = 0.017) or sod2 (r2 = 0.049, p = 0.09) mRNA expressions and ALA concentration. This study demonstrates that diet can affect concentrations of ALA and other fatty acids as well as change activities and gene expression of antioxidant enzymes in muscle. Increased antioxidant activity may, in turn, have beneficial effects on the performance, health and wellbeing of animals and humans.
Post Meal Energy Boluses Do Not Increase the Duration of Muscle Protein Synthesis Stimulation in Two Anabolic Resistant Situations
Authors: Mosoni Laurent , Jarzaguet Marianne , David Jérémie , Polakof Sergio , Savary-Auzeloux Isabelle , Rémond Didier , Dardevet Dominique
Published: March 2019

Background: When given in the long term, whey proteins alone do not appear to be an optimal nutritional strategy to prevent or slow down muscle wasting during aging or catabolic states. It has been hypothesized that the digestion of whey may be too rapid during a catabolic situation to sustain the anabolic postprandial amino acid requirement necessary to elicit an optimal anabolic response. Interestingly, it has been shown recently that the duration of the postprandial stimulation of muscle protein synthesis in healthy conditions can be prolonged by the supplementary ingestion of a desynchronized carbohydrate load after food intake. We verified this hypothesis in the present study in two different cases of muscle wasting associated with anabolic resistance, i.e., glucocorticoid treatment and aging. Methods: Multi-catheterized minipigs were treated or not with glucocorticoids for 8 days. Muscle protein synthesis was measured sequentially over time after the infusion of a 13C phenylalanine tracer using the arterio-venous method before and after whey protein meal ingestion. The energy bolus was given 150 min after the meal. For the aging study, aged rats were fed the whey meal and muscle protein synthesis was measured sequentially over time with the flooding dose method using 13C Valine. The energy bolus was given 210 min after the meal. Results: Glucocorticoid treatment resulted in a decrease in the duration of the stimulation of muscle protein synthesis. The energy bolus given after food intake was unable to prolong this stimulation despite a simultaneous increase of insulin and glucose following its absorption. In old rats, a similar observation was made with no effect of the energy bolus on the duration of the muscle anabolic response following whey protein meal intake. Conclusions. Despite very promising observations in healthy situations, the strategy aimed at increasing muscle protein synthesis stimulation by giving an energy bolus during the postprandial period remained inefficient in our two anabolic resistance models.
Difficulties in Translating Appetite Sensations Effect of Turmeric-Based Beverage When Given Prior to Isoenergetic Medium- or High-Fat Meals in Healthy Subjects
Authors: Zanzer Yoghatama , Batista Ângela , Dougkas Anestis , Tovar Juscelino , Granfeldt Yvonne , Östman Elin
Published: March 2019

The established effect of turmeric and its curcuminoids on appetite sensations was previously shown to be mediated by gut hormones release. In in vitro and preclinical studies, curcumin was shown to induce GLP-1 secretion and improve postprandial glycemia. In humans, consumption of 220 mL turmeric-based beverage (TUR, containing 185 mg gallic acid equivalents (GAE)) prior to white wheat bread (WWB, 50 g available carbohydrate) reduced early postprandial glucose levels and induced peptide tyrosine–tyrosine (PYY) release, as well as lowered ‘desire to eat’ and ‘prospective consumption’ in a postprandial setting, compared to control. In the present study, 12 healthy participants (5 men, 7 women) were admitted. An identical beverage was given and consumed prior to isoenergetic (423 kcal) medium-fat (MF) or high-fat (HF) meals. Appetite sensations including perceived ‘hunger’, ‘desire to eat’, ‘satiety’, ‘fullness’, ‘prospective consumption’, and ‘thirst’ were measured using visual analogue scales. MF induced 18% (p = 0.039) higher ‘satiety’ compared to HF. TUR consumption prior to either MF or HF did not modulate the perceived appetite sensations. Whether macronutrient-induced appetite sensations override the actual turmeric effects warrants further investigation.
Genetic Risk Score Predictive of the Plasma Triglyceride Response to an Omega-3 Fatty Acid Supplementation in a Mexican Population
Authors: Vallée Marcotte Bastien , Guénard Frédéric , Marquis Julien , Charpagne Aline , Vadillo-Ortega Felipe , Tejero Maria Elizabeth , Binia Aristea , Vohl Marie-Claude
Published: March 2019

Our group built a genetic risk score (GRS) of the plasma triglyceride (TG) response to an omega-3 (n-3) fatty acid (FA) supplementation in Caucasian Canadians that explained 21.53% of the TG variance. The objective was to refine the GRS by fine mapping and to test its association with the TG response in young Mexican adults. A total of 191 participants underwent a 6-week n-3 FA supplementation providing 2.7g/day of docosahexaenoic and eicosapentaenoic acids. Using quantitative polymerase chain reaction (PCR), 103 single-nucleotide polymorphisms (SNPs) were genotyped. A stepwise regression adjusted for age, sex, and body mass index (BMI) was used to select the strongest SNPs to include in the genetic risk model. A GRS was calculated from the sum of at-risk alleles. The contribution of the GRS to the TG response was assessed by ANCOVA with age, sex, and BMI included in the model. Several differences in allele frequency were observed between Canadians and Mexicans. Five lead SNPs were included in the genetic risk model, in which the GRS accounted for 11.01% of the variance of the TG response (p < 0.0001). These findings highlight the important contribution of genetic factors to the heterogeneity of the TG response to an n-3 FA supplementation among Mexicans.