bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of Storage Time at 2–8 Degrees Celsius on the Stability of von Willebrand Factor in Thawed, Platelet-Poor Plasma
Authors: Nakkinkun Yupa , Binhama Tussnem , U-pratya Yaowaluk , Rungjirajittranon Tarinee , Ruchutrakool Theera
Published: November 2023

thawed samples with plasma stored at 2–8 °C for 24–96 hours.
Materials and Methods: Plasma from healthy subjects with normal coagulation times and VWF panels was stored at -20 °C for one week. After thawing (at 0 hours), VWF:antigen (VWF:Ag), VWF:glycoprotein Ib binding assay (VWF:GPIbM), and VWF:collagen binding assay (VWF:CB) were assayed. The remaining plasma was stored at 2–8 °C and assayed at 24, 48, 72, and 96 hours. Differences between levels at baseline and 24, 48, 72, and 96 hours were deemed significant when P was < 0.05.
Results: Thirty-five samples were enrolled, with 25 from healthy subjects (VWF:Ag levels > 0.50 kIU/L). Median levels (interquartile range) were as follows: VWF:Ag = 0.91 (0.72–1.06) kIU/L; VWF:GPIbM = 0.85 (0.69–1.04) kIU/L; and VWF:CB = 0.78 (0.62–0.97) kIU/L. VWF:Ag remained stable for 72 hours, while VWF:GPIbM decreased significantly after thawing. VWF:CB declined after 48 hours at 2–8 °C. Similar stability trends were observed in 10 additional samples from VWD patients (VWF:Ag = 0.42 (0.36–0.46) kIU/L).
Conclusion: VWF:Ag and VWF:CB are stable in thawed plasma for 72 hours. VWF:GPIbM is less stable and should not be kept longer than 24 hours. Immediate testing of VWF:GPIbM after thawing is recommended.
Evaluating Risk Factors for Cumulative Life Course Impairment in Psoriasis using Patient-Acceptable Symptom State and European Quality of Life 5 Dimensions (EQ-5D)
Authors: Chularojanamontri Leena , Wongpraparut Chanisada , Silpa-archa Narumol , Chaiyabutr Chayada , Wongdama Supisara , Chiowchanwisawakit Praveena
Published: November 2023

Objective: To evaluated the health-utility values and risk factors for cumulative life course impairment (CLCI) using Patient Acceptable Symptom State (PASS) and European Quality of Life 5 Dimensions (EQ-5D).
Materials and Methods: This cross-sectional investigation enrolled patients with psoriasis. Patients were asked PASS questions about their overall self-perceived health state, adaptation, and expectations for current, future, and lifelong conditions. The patients also completed EQ-5D.
Results: The mean age of 139 enrolled patients was 45.8 ± 14.4 years, and 57.6% were women. Most cases had chronic plaque psoriasis 121 (87.1%). For current PASS, satisfaction was significantly associated with older age, being married, and lower disease severity. The mean health-utility value and visual analog scale of the 139 patients were 0.89 ± 0.12 and 77.0 ± 17.4, respectively. Patients would not accept their disease if they had moderate to extreme problems in usual activities and depression/anxiety for the future and lifelong. Univariate analysis revealed that depression and usual activities were significantly associated with satisfaction for current PASS, future PASS, and lifelong PASS.
Conclusion: Disease severity, age, marital status, problems with usual activities, and depression/anxiety were significantly related to CLCI. These findings may allow physicians to identify psychosocial and psychological aspects of psoriatic patients at high risk for developing CLCI. Early and adequate treatment, good coping strategies, and good social support can prevent a negative impact on CLCI and major life-changing decisions.
Validity and Reliability of a Thai Behavioral and Emotional Screening Tool for Children with Enuresis (TBEST-E)
Authors: Manomaivong Varis , Wannapaschaiyong Prakasit , Sirisakpanit Sudarat , Kantasorn Jeeranan , Tarugsa Jariya , Piyaphanee Nuntawan , Chantaratin Sasitorn
Published: November 2023

Objective: Psychological comorbidity in children with enuresis is common and its screening is recommended. A brief validated screening instrument is needed for a busy medical practice. This study focused on the development of the Thai Behavioral and Emotional Screening Tool for children with Enuresis (TBEST-E) and aimed to examine its psychometric properties.  
Materials and Methods: Using the Short Screening Instrument for Psychological Problems in Enuresis (SSIPPE) as a template, the 15-item with a yes/no answer format of the TBEST-E was developed for the screening of emotional problems (7 items), attention and hyperactivity/impulsivity problems (3 items each), and oppositional defiant symptoms (2 items). The parents of the children with enuresis completed the TBEST-E and the behavioral rating scale “Thai Youth Checklist” (TYC). The optimal cut-off for further assessment, the sensitivity, and the specificity the TBEST-E were identified using receiver operating characteristic (ROC) curves.  
Results: After an adaptation following comments from three child psychiatrists, the TBEST-E showed a content validity of 0.9, an internal consistency of 0.71. A total of 33 children with enuresis (median age = 9, interquartile range = 6.5-11 years) were recruited. Further assessment was indicated when one of the following occurred; at least 3 emotional problems; 3 attention problems; or 2 hyperactivity/impulsivity together with 2 oppositional defiant symptoms. The overall sensitivity and specificity were 0.88 and 0.71 respectively with the accuracy of 0.84 (95% CI = 0.68-0.95).
Conclusion: The TBEST-E is time-efficient and has acceptable psychometric properties in early detection of common psychological problems in children with enuresis.
t(11;14) status is stable between diagnosis and relapse, and concordant between detection methodologies based on fluorescence in situ hybridization and next-generation sequencing in patients with multiple myeloma
Authors: Avet-Loiseau Hervé , Thiébaut-Millot Raphaële , Li Xiaotong , Ross Jeremy A. , Hader Carlos
Published: November 2023

Multiple myeloma (MM) is associated with a wide variety of recurrent genomic alterations. The most common translocation in MM is t(11;14). In this retrospective, single-center, non-interventional study, patient bone marrow samples were examined at diagnosis and at relapse(s) following treatment with anti-myeloma regimens to determine whether t(11;14) status was stable over time. This Stability Cohort consisted of 272 patients, of whom 118 were t(11;14)-positive at diagnosis and 154 were negative. All patients in the Stability Cohort retained the same t(11;14) status at relapse that they had at diagnosis of MM. Sixteen patients who had t(11;14)-positive MM at diagnosis had multiple longitudinal FISH assessments at relapse events, which remained t(11;14)-positive across all time points. Patients who had t(11;14)-positive disease at diagnosis of monoclonal gammopathy of unknown significance (MGUS) or smoldering multiple myeloma (SMM) also retained t(11;14) positivity through MM diagnosis and relapse. The t(11;14) fusion patterns also remained constant for 90% of patients. For detection of t(11;14), results from fluorescence in situ hybridization (FISH) and next generation sequencing (NGS) were compared to determine the rate of concordance between these 2 methods. This Concordance Cohort contained 130 patients, of whom 66 had t(11;14)-positive disease and 64 were t(11;14)-negative. In this sample set, the concordance between FISH and NGS-based detection of t(11;14) was 100%. These results strongly suggest that the t(11;14) rearrangement remains stable during the full disease course in patients with multiple myeloma and can be detected by FISH- and NGS-based methodologies.
Вплив комплексної фармацевтичної композиції на показники системи ПОЛ-АОЗ печінки за умов експериментального метаболічного синдрому в хом’яків
Authors: Калько К. О. , Духніч Н. Ю.
Published: November 2023

Сьогодні спосіб життя багатьох людей, який називають сучасним західним стилем життя, при­ зводить до поширеності метаболічного синдрому (МС), своєчасна профілактика та лікування якого є край важливою задачею.

Мета дослідження – встановити вплив комплексної фармацевтичної композиції (КФК) (Aevit premium) на показники системи ПОЛ­АОЗ за умов експериментального МС (ЕМС) у сирійських золотавих хом’яків.

МС у сирійських золотавих хом’яків індукували кафе­дієтою, складовими якої були суміш з про­ мислово­оброблених харчових продуктів з вмістом жирів не менше ніж 40 %. Приготовлену суміш давали тваринам з надлишком упродовж 7 тижнів (49 днів). Питну воду було замінено на 10 % р­н фруктози. Досліджувану КФК (25,8 мг/кг) і препарати порівняння метформін (60,0 мг/кг) і вітамін Е (100 мг/кг) застосовували внутрішньошлунково, починаючи з 5 тижня моделювання МС упродовж 3 тижнів (21 день), тобто, у лікувальному режимі.

Тривале перебування хом’яків групи контрольної патології на раціоні кафе­дієти призводило до розвитку МС, дисбалансу в системі ПОЛ­АОЗ, про що свідчило зниження вмісту відновленого глута­ тіону (ВГ), активності супероксиддисмутази (СОД) та каталази в тканині печінки та на тлі зростання вмісту тіобарбітурової кислоти активних продуктів (ТБК­АП). Досліджувана КФК сприяла відновлен­ню балансу в системі ПОЛ­АОЗ: збільшення вмісту ВГ, активності СОД та каталази та одночасне зменшення вмісту ТБК­АП. Встановлена здатність КФК пригнічувати оксидативний стрес та сприяти нормалізації балансу в системі ПОЛ­АОЗ обумовлена реалізацією прямої та непрямої антиокси­ дантної дії її складових (етиловими ефірами омега­3 кислот, вітаміном Е, коензимом Q10, цинком, вітаміном А, біотином, селеном), а також спроможністю останніх чинити метаболітотропну дію. За виразністю коригуючого впливу на стан системи ПОЛ­АОЗ печінки хом’яків в умовах ЕМС КФК пере­ важала препарати порівняння метформін та вітамін Е.

Отримані результати обґрунтовують доцільність застосування КФК у комплексній фармакокорек­ ції МС.
Influence of Nutritional Status of Children with Acute Lymphoblastic Leukemia (ALL) on Treatment Outcome after Completion of Induction
Authors: Rahman SM Rezanur , Jamal Chowdhury Yakub , Khasru Akm Amirul Morshed , Khan Zohora Jameela , Akter Mehnaz , Ara Zannat , Jhahan Hosna
Published: November 2023

Background: Malnutrition and cancer both influences tolerance and response to treatment especially ALL. Malnutrition increases morbidity, mortality, reduces the effectiveness of treatment and impairs the quality of life significantly.
Objective: Assessment of the nutritional status and its influence on treatment outcome children with Acute Lymphoblastic Leukemia (ALL) before and after induction chemotherapy.
Materials & Methods: This prospective observational study included 72 children with ALL, aged 1-15 years, was conducted from January to December 2014 in the Department of Pediatric Hematology and Oncology, BSMMU. The anthropometric measurements (weight-for-age, height-for-age, weight-for-height, MUAC, BMI) hematological and biochemical parameter (serum albumin, total protein) were measured. The children got induction chemotherapy according to the UK ALL 2003 (modified) protocol.
Results: Among the study population, 18(39.1%) were underweight, 8(17.4%) were stunted, 16(34.8%) were wasted, MUAC for age 14(56.0%) and BMI for age 12(57.1%) were malnourished, 10.9% had low total proteins (<5.7g/dl), 37.0% low serum albumin (<3.2g/dL). Mean anthropometric measurements and biochemical parameters were higher among the survivors compared to non-survivors. Significant difference was found between the well-nourished and the malnourished group according to WAZ. Complications like febrile neutropenia, treatment delay, number of transfusion (PRBC) requirement were more in malnourished group.
Conclusion: Malnutrition is widely prevalent in children with ALL and a major determining factor in treatment planning, complications and final outcome.
BANGLADESH J CHILD HEALTH 2022; VOL 46 (1) : 3-8
Newly Diagnosed Type 2 Diabetes in Children and Adolescents in Bangladesh- A Cross Sectional Study
Authors: Nahar Jebun , Zabeen Bedowra , Ahmed Bulbul , Ahmed Tasnima , Islam Nasreen , Azad Kishwar
Published: November 2023

Background: Type 2 diabetes (T2D) has become a serious health problem in children and adolescents as the prevalence of obesity is increasing.
Objective: To observe the baseline clinical and laboratory findings and treatment modalities of children and adolescents with newly diagnosed T2D.
Materials & Methods: This cross-sectional study was done from Jan- Dec.2017 in Changing Diabetes in Children (CDiC), Paediatric diabetes OPD clinic in BIRDEM among newly diagnosed T2D children and adolescents.
Results: Among 68 patients majority were female (64.7%). Mean age was 13.1 ± 2.5 years. Ten patients were below 10 years. Most of the patients were from urban & semi urban area and high to middle socioeconomic status. Family history was present in 91.2% and 22.1 % mother had H/O GDM. Forty two patients (61.8%) had typical and 22 (32.3%) had atypical symptoms. Thirty seven children had acanthosis nigricans (54.4%). Majority were found overweight (53/68), and (12/68) were obese. Mean HbA1c was 10.2 ± 2.8. Life style modification was advised to all. Insulin was started along with metformin in patients with high HbA1c and 33.8% patients were given metformin only. While comparing, BMI was found high in basal and metformin group. Lower fasting blood glucose and HbA1c were observed in patients with metformin only.
Conclusion: T2D is emerging in children and adolescents. The screening of children at high risk of T2D is essential for preventive and timely remedial measures.
BANGLADESH J CHILD HEALTH 2022; VOL 46 (1) : 9-13
Efficacy of Combined Therapy of ACTH plus Vigabatrin Compared to ACTH Alone for Treatment of Infantile Spasm-A RCT
Authors: Sumi Samsun Nahar , Mistry Banita , Habib Md Ahsan , Chandra Mahua , Hoque Seikh Azimul , Saha Narayan Chandra
Published: November 2023

Background: Infantile spasms (epileptic spasm) is difficult to treat and has a high morbidity. So it’s crucial to initiate an early effective therapy. ACTH, Prednisolone and Vigabatrin have shown better efficacy on cessation of spasms.

Objective: To compare the efficacy and tolerability of combined ACTH and Vigabatrin with ACTH alone in treatment of infantile spasms.

Materials & Methods: This randomized controlled trial was conducted from June, 2017 to June, 2018 at outdoor and indoor, department of Paediatric Neurology of National Institute of Neurosciences and Hospital, Dhaka. Fifty (50) patients aged 2 months to 24 months who had a clinical diagnosis of infantile spasms and hypsarrhythmia (classical or modified hypsarrhythmia) in EEG were enrolled. With parent’s written informed consent, they were randomized (1:1 by lottery method) into two groups, 25 in each. One group got ACTH only and another group got both ACTH and vigabatrin. At the end of the treatment two groups were compared regarding efficacy and safety of the drugs.

Result: The primary outcome was assessed in 50 children at 91st day. The primary outcome was cessation of spasms from 14th day to 42nd day after initiation of therapy. Cessation of spasms (between 14 to 42 days) occurred in 18 children (72%) in combination therapy and 11 children (44%) in hormonal therapy (p=0.045). Treatment response was faster on combination therapy (p=0.001). Treatment was well tolerated in both groups with few adverse effects.

Conclusion: Combination therapy of ACTH plus Vigabatrin was found better than ACTH therapy alone in cessation of infantile spasms.

BANGLADESH J CHILD HEALTH 2022; VOL 46 (1) : 14-18
Anti-tuberculosis Drug-induced Hepatitis
Authors: Karim ASM Bazlul , Musabbir Nadira
Published: November 2023

Among the first line anti-TB drugs, INH, Rifampicin and Pyrazinamide are considered to be the most hepatotoxic drugs and cause drug induced hepatitis. Pyrazinamide causes both dose-dependent and idiosyncratic hepatitis whereas Isoniazid and Rifampicin induced hepatotoxicity are considered to be idiosyncratic. There are several risk factors for hepatotoxicity such as old age, malnutrition, genetic predisposition, HIV infection as well as chronic Hepatitis B and C infections. Anti-TB drug induced hepatitis usually occurs within 2 months after starting treatment. Patient may present with mild symptoms to severe acute hepatitis or even acute liver failure. Various guidelines have been published regarding the management of ATT and restarting anti TB drugs. This article reviews the incidence, risk factors, mechanism, diagnosis and management strategies of anti-TB drug induced hepatitis.

BANGLADESH J CHILD HEALTH 2022; VOL 46 (1) : 23-27
Idiopathic Short Stature - A Case Report
Authors: Begum Suraiya , Biswas Dhiraj Chandra
Published: November 2023

Short stature in childhood is the most common reason for referral to pediatric endocrinologists. Idiopathic short stature is defined as ³2 SD below the corresponding mean height for a given age, sex, and population in a child with a normal birth size and normal body proportions and without evidence of any systemic, endocrine, nutritional, or chromosomal abnormalities. ISS children have normal GH responses to stimulation test and GH therapy has approved for the treatment of children with ISS. A six-year-old girl presented with not growing well in comparison to other peers since 6 months of her age. Her WAZ was -4.5 SD, HAZ was -7.7 SD, and projected height is far below parental target height. Bone age was 2-3 year and growth hormone stimulation showed normal growth hormone secretion. Here we reported a six-yearold girl diagnosed as a case of non-familial ISS and treated with growth hormone. Diagnosis of Idiopathic short stature was done by exclusion of other causes of short stature and growth hormone therapy was indicated.

BANGLADESH J CHILD HEALTH 2022; VOL 46 (1) : 28-29
Liệu chính sách có ảnh hưởng đến quyết định cấp tín dụng cho các dự án xanh? – Bằng chứng thực nghiệm tại Việt Nam
Authors: Lê Đức Lữ , Khúc Thế Anh , Bùi Kiên Trung
Published: November 2023

Bài viết này tập trung trả lời câu hỏi: chính sách có ảnh hưởng đến việc lập kế hoạch và tài trợ cho các dự án xanh của các tổ chức tài chính không phải ngân hàng hay không? Nghiên cứu định tính được sử dụng để phỏng vấn các chuyên gia lý thuyết và thực tiễn nhằm hiệu chỉnh bảng hỏi, thang đo và thảo luận một số chính sách. Chúng tôi sử dụng SPSS26 và AMOS24 để xử lý 1.383 bảng hỏi thu thập từ khảo sát. Kết quả cho thấy chính sách của các cơ quan quản lý nhà nước và của chính các tổ chức có ảnh hưởng tích cực đến ý định và quyết định cấp tín dụng xanh. Dựa trên kết quả nghiên cứu, chúng tôi đưa ra một số hàm ý chính sách đến Chính phủ, ngân hàng nhà nước, các Bộ có liên quan (như Bộ Kế hoạch và Đầu tư; Bộ Tài chính) và chính các tổ chức tài chính.
Bantuan Teknis Dalam Penyusunan Rencana Anggaran Biaya (Rab) Program Kerja Desa Berbasis Dana Desa
Authors: Saputra Taufiq Yuda , Ahadian Edward Rizky , Amal Ihlasul
Published: November 2023

Pulau MaitaraÂ  merupakan salah satu kawasan wisata di Kota Tidore Kepulauan Provinsi Maluku Utara yang terletak diantara Pulau Tidore dan sekatan Pulau Ternate adalah suatu bongkahan pulau kecil yang alamiah berpenduduk 1900 jiwa denga keramahtamaan, budaya dan jiwa sosialnya yang tinggi serta menyimpang kekayaan yang potensial untuk pengembangan sebagai kawasan wisata. Dengan demikian, berdasarkan potensi yang ada di daerah ini, kegiatan dariÂ  PKM ini sangat berpeluang besar sebaggai upaya peningkatan ekonomiÂ  di tingkat keluarga guna mewujudkan kesejahteraan desa. Dalam pelaksanaannya, dana desa mulai dialokasikan dalam APBN sejak 2015 sebesar Rp. 20,76 triliun. Dalam periode 2015-2020, alokasi dana desa melalui APBN telah mencapai Rp. 328,07 triliun. Pagu Dana Desa Tahun 2022 telah ditetapkan sebesar Rp. 68 triliun dan dialokasikan kepada 74.961 desa di 434 kabupaten/kota se-Indonesia. Berdasarkan pantauan, selama tahun 2021, secara umum permasalahan dana desa dapat dilihat dari aspek perencanaan, pengelolaan dan pertanggungjawaban. Hal ini menunjukkan bahwa pendampingan dan pengawasan pengelolaan dana desa belum berjalan dengan optimal. Oleh karena itu, peningkatan pembinaan, pendampingan dan pengawasan pengelolaan dana desa menjadi kewajiban yang harus DilakukanKata Kunci: Dana Desa, Program Kerja
Penyuluhan Rumah Sehat di Moti
Authors: Quraisy Sayyid , Tayeb Mustamin Muhammad
Published: November 2023

ABSTRAKRumah merupakan kebutuhan dasar manusia yang berfungsi sebagai tempat berteduh dan melakukan kegiatan sehari-hari dalam keluarga. Rumah tidak sekedar sebagai tempat untuk melepas lelah setelah bekerja seharian, namun didalamnya terkandung arti yang penting sebagai tempat untuk membangun keluarga sehat dan sejahtera. Rumah dikatakan sehat menurut Dinkes (2007), apabila memenuhi kriteria yaitu: (1) memenuhi kebutuhan fisiologis; (2) memenuhi kebutuhan psikologis; (3) memenuhi persyaratan pencegahan penularan penyakit antar penghuni rumah dan; (4) memenuhi persyaratan pencegahan terjadinya kecelakaan. Penyuluhan dilaksanakan secara langsung dengan menghadirkan warga setempat di Desa Moti.Kata Kunci: Penyuluhan, Rumah, Sehat, Moti
Sosialisasi dan Edukasih Zona Selamat Sekolah Pada Murid Kelas V SD Mononutu Kota Ternate
Authors: M. Nurmaiyasa , Gaus Abdul , Amalia Ema
Published: November 2023

Pendidikan mengenai keselamatan berlalu lintas harus ditanamkan sejak dini di kalangan anak-anak sekolah. Untuk itu Pemerintah pun memberi perhatian khusus terhadap anak-anak usia sekolah ini yang ditandai dengan inisiatif untuk menggulirkan program Zona Selamat Sekolah (ZoSS) yaitu dengan memberikan edukasi serta dukungan sarana dan prasarana dalam penyelenggaraan perlindungan anak. Lokasi yang dipilih dalam pengabdian kepada masyarakat ini adalah sekolah dasar yang lokasi sekolahnya masuk dalam kategori rawan lalu lintas, lokasinya berada pada SD Mononutu kota TernateÂ Ketersedian Zona Selamat Sekolah (ZoSS) di SD Mononutu belum berfungsi maksimal, ini terlihat Â masih adanya terjadi kecelakaan ringan maupun berat yang terjadi di seputaran ruas jalan sekolah, dari hasil sosialisasi pemaparan zona selamat sekola yang di sampikan ke pada siswa SD kelas V hampir seluruh murid kelas V belum paham dan mengerti tentang atribut dan rambu-rambu zona selamat sekolah serta fungsinya-fungsi dari marka dan rambu-rambu yang ada di lingkungan ruas jalan sekolah yang berada di lokasi sekolah mereka, hasil dari sosialisasi Â dan edukasi Zona Selamat Sekolah diharapkan siswaseluruh siswa SD Mononuti dapat memahami dan mengerti tentang edukasi fungsi dan kegunaan zona selamat sekolah, serta memberikan rasa aman kepada para murid yang akan menyeberang di jalan. DiharapkanÂ  dengan adanya kegiatan PKM dapat membantu penerapan tentang zona selamat sekolah dan dapat meminimalisir kecelakaan lalu lintas, yang ada di ruas jalan depan sekolah mereka.Kata Kunci: Zona, Selamat, Sekolah, Murid
Dampak Penggunaan Gadget Terhadap Perkembangan Anak Usia Dini di SD Insantama Kota Ternate
Authors: Ahmad Badrun , Misbah Zulkarnain K , Y.S Muhammad Taufiq
Published: November 2023

Sosialisasi dampak kecanduan penggunaan hp kepada siswa-siswi SD insantama melibatkan beberapa kelas dari kelas 4 sampai kelas 6. PKM ini bertujuan untuk memberikan informasi positif kepada siswa-siswa SD Insantama agar tidak kecanduan menggunakan handphone. Informasi yang disosialisasikan yaitu bahaya kecanduan penggunaan hp. Kecanduan penggunaan hp memberikan efek fisik dan efek psikologi. Sosialisasi ke depan terkait bahaya kecanduan penggunaan hp kepada siswa-siswi SD Insantama perlu dilakukan secara rutin oleh guru-guru SD Insantama. Sosialiasi ini perlu melibatkan program studi psikologi untuk melakukan pendampingan saat sosialisasi. Kegiatan sosialisasi perlu didukung dengan media dan alat-alat bantu yang lebih lengkap sehingga siswa-siswi lebih memahami efek negatif kecanduan penggunaan hp.Kata Kunci: dampak, gadget, anak, insantama, sosialisasi, kelas, ternate.
TINGKAT KECEMASAN KLIEN GAGAL GINJAL TERMINAL PADA TINDAKAN HEMODIALISIS
Authors: Taryana Abay
Published: November 2023

Tindakan hemodialisis (HD) pada klien gagal ginjal terminal dianggap sebagai sumber stressor sehingga rentan menimbulkan kecemasan yang dapat menurunkan adekuasi HD. Penelitian ini dilakukan untuk mengidentifikasi tingkat kecemasan di setiap tahapan tindakan HD yaitu sebelum, intra dan setelah HD di Rumah Sakit Al Islam Bandung. Metode penelitian deskriptif kuantitatif. Pengambilan sampel dilakukan dengan teknik accidental samplingselama 6 hari dan didapatkan 119 responden. Pengumpulan data menggunakan State Trait Anxiety Inventory (STAI) for Adult Form Y. Data hasil penelitian dianalisis dengan analisis deskriptif kuantitatif. Hasil penelitian menunjukkan bahwa tingkat state anxietysebagian besar klien HD termasuk dalam kategori cemas ringan. Skor mean state anxietyuntuk setiap tahapan tindakan HD adalah sebelum HDmean(SD)=37,7(11,2), intra HDmean(SD)=35,2(9,4) dan post HD mean(SD)=36,3(10,7)]. Sedangkan tingkat trait anxietysebagian besar klien dalam kategori cemas sedang. Skor mean trait anxiety untuk setiap tahapan tindakan HD adalah sebelum HD mean(SD)=42,1(9,3), intra HD mean(SD)=41,6(9,5) dan setelah HD mean(SD)=40,9(9,9).Berdasarkan penelitian ini skor tertinggi untuk state anxietydan trait anxietyditemukan pada tahap sebelum HD, sehingga diperlukan penatalaksanaan yang tepat misalnya dengan memodifikasi ruang HD untuk meningkatkan kenyamanan klien, penggunaan teknik relaksasi dan distraksi sebelum dan saat pelaksanaan tindakan akses vaskuler untuk mengurangi dan mencegah kecemasan klien HD.
Kesiapan Perawat Gawat Darurat Rumah Sakit Umum Daerah Kabupaten Bandung Dalam Menghadapi Bencana
Authors: Lestari Diah Ayu Sri
Published: November 2023

Bencana merupakan peristiwa yang mengancam serta mengganggu kehidupan masyarakat. Respon cepat dalam situasi tanggap bencana merupakan hal penting yang dilakukan oleh seorang first responder untuk meminimalisir korban jiwa. Kesiapan perawat merupakan hal krusial, termasuk kesiapan perawat sebagai first responder bencana dalam fase tanggap darurat. Sementara dalam pelaksanaannya, perawat tidak melakukan implementasi secara maksimal. Hal ini diperkuat oleh sebuah penelitian yang mengatakan bahwa perawat tidak memiliki persiapan yang baik dalam penanganan bencana. Penelitian ini bertujuan untuk mengetahui kesiapan perawat gawat darurat RSUD Kabupaten Bandung dalam menghadapi bencana.
Penelitian ini menggunakan metode deskriptif kuantitatif dengan jumlah populasi sebanyak 33 perawat IGD RSUD Soreang dan RSUD Majalaya dengan menggunakan teknik total sampling. Penelitian menggunakan instrumen Emergency Preparedness Information Questionnaire (EPIQ) yang membagi kesiapan perawat menjadi 8 dimensi dengan jumlah 24 item pernyataan diukur menggunakan skala likert dan hasil pengukurannya dihitung dengan menggunakan analisis deskriptif.
Hasil penelitian ini menunjukkan bahwa sebanyak 85% perawat memiliki kesiapan sedang dalam menghadapi bencana, dengan skor mean tertinggi (3,16) berada pada dimensi sistem komando kejadian dan terendah (2,80) berada pada populasi khusus.
Kesiapan perawat berada dalam kesiapan sedang berarti dikatakan sudah cukup siap terlebih dalam sistem komando kejadian. Meskipun ada dimensi yang masih kurang, yaitu populasi khusus. Hal ini dapat dikarenakan penanganan pada populasi khusus paling banyak memerlukan perhatian pada perawatannya dan kegagalan dalam penanganannya akan berdampak kurang baik bagi kehidupan mereka selanjutnya. Maka untuk memenuhi kebutuhan tersebut dapat dilakukan program pelatihan penanganan populasi khusus.
 
Kata Kunci      : Bencana, EPIQ, Kesiapan Perawat
Pengaruh Metoda Ceramah Dan Demonstrasi Terhadap Pengetahuan Kader Kesehatan Mengenai Perawatan Infeksi Saluran Nafas Akut (Ispa) Dan Deteksi Dini Pneumonia Pada Balita di Cikijing Kabupaten Majalengka
Authors: Adistie Fanny , Nurhidayah Ikeu , Mardhiyah Ai , Hendrawati Sri , Maryam Nenden Nur Asriyani
Published: November 2023

Di Indonesia, penyakit infeksi saluran pernapasan akut (ISPA) menjadi fokus program kesehatan terutama pneumonia karena merupakan penyebab utama mortalitas anak balita di Indonesia. Maka dari itu, mengoptimalkan peran kader kesehatan dapat membantu masyarakat untuk dapat melakukan perawatan ISPA dengan benar serta mendeteksi adanya tanda bahaya dari ISPA tersebut seperti pneumonia. Tujuan dari penelitian ini adalah untuk mengidentifikasi pengaruh metode ceramah dan demonstrasi terhadap pengetahuan kader kesehatan mengenai perawatan ISPA dan deteksi dini pneumonia pada anak. Penelitian ini menggunakan desain penelitian pre-eksperimental dengan one group pretest-posttest. Responden pada penelitian ini sebanyak 39 kader kesehatan, dengan intervensi yang dilakukan adalah pelatihan pada kader kesehatan mengenai perawatan ISPA dan deteksi dini pneumonia pada anak dengan menggunakan metode ceramah serta demonstrasi. Hasil penelitian didapatkan sebelum dilakukan intervensi pengetahuan kader kesehatan menunjukkan 44,4% responden memiliki pengetahuan yang cukup dan sebagian besar responden 55,6% responden memiliki pengetahuan yang kurang. Setelah dilakukan intervensi pengetahuan kader kesehatan menunjukkan sebagian besar 77,8% responden memiliki pengetahuan yang baik dan hanya 22,2% responden yang memiliki pengetahuan yang cukup. Hasil Uji dengan Wilcoxon didapatkan pengaruh yang signifikan dari metode ceramah dan demonstrasi terhadap pengetahuan kader kesehatan mengenai perawatan ISPA dan deteksi dini pneumonia pada anak  (p = 0.000). Implikasi dari penelitian ini adalah metode ceramah yang dikombinasikan dengan demonstrasi terbukti efektif untuk meningkatkan pengetahuan para akder kesehatan, maka dari itu metode pada intervensi ini dapat digunakan dalam memberikan pelatihan kepada para kader kesehatan.
Impact of food processing on the allergenic properties of amylase trypsin inhibitors from wheat
Authors: Weegels Peter L. , America Antoine H. P.
Published: November 2023

Amylase trypsin inhibitors (ATIs) play an important role in wheat allergies and potentially in non-coeliac wheat sensitivity. Food processing could be important to mitigate the pathogenic properties of ATIs, e.g., by denaturation, glycation, enzymatic hydrolysis, cross-linking, and oxidation and reduction. These modifications also impact the solubility and extractability. The complex solubility behaviour of ATI isoforms (water and salt soluble, but also chloroform–methanol soluble, solubility depending on the redox state) becomes even more complex upon processing due to denaturation and (bio)chemical modifications. This significantly hinders the feasibility of quantitative extraction. Moreover, changes in biofunctionality may occur during the process of extraction, and the changes in ATI due to food processing will be more difficult to assess. Heat treatment decreases the extractability of ATIs with water, NaCl, and other buffer extracts, and binding of IgE from wheat-allergic persons to ATIs as observed with Western blotting is decreased or absent. IgE binding is reduced with the total extract in chaotropic and reducing agents. However, it can be increased when the proteins are hydrolyzed by proteases. Fermentation involving certain species of Fructolactobacilli (FLB), followed by baking, decreases the amount of ATIs and IgE binding to ATIs. In yeast-fermented bread, the amount of ATIs decreased in a similar manner, but IgE binding was more prominent, indicating that there was a modification of ATIs that affected the epitope recognition. When isolated ATIs are ingested with high ATI degrading FLB, the immune response in mice is less elevated in vivo, when compared with ATI without high ATI degrading FLB. The pathogenic effects on the skin of dogs and one wheat-allergic child are also decreased when soluble proteins or isolated ATIs are reduced with the thioredoxin/thioredoxin reductase NADPH system. Glycation on the other hand has been shown to potentiate the allergenic properties of ATIs as evidenced by the large increase in IgE binding. The impact of food processing on the pathogenic properties of ATIs is hardly studied in vivo in humans. There seem to be opportunities to mitigate the pathogenic properties in vitro, but potentiation of pathogenic properties is also frequently observed. This requires a deeper understanding on the impact of food processing on the pathogenicity of ATIs.
Establishing a three-dimensional scaffold model of hepatoblastoma
Authors: Weigl Elena Johanna , Demir Salih , Schmid Tanja , Hotes Alina , Muensterer Oliver , Kappler Roland
Published: November 2023

Introduction: Emerging technologies such as three-dimensional (3D) cell culture and the generation of biological matrices offer exciting new possibilities in disease modelling and tumour therapy. The paucity of laboratory models for hepatoblastoma (HB), the most prevalent malignant liver tumour in children, has hampered the identification of new treatment options for HB patients. We aimed to establish a reliable 3D testing platform using liver-derived scaffolds and HB cell lines that reflect the heterogeneous biology of the disease so as to allow reproducible preclinical research and drug testing.

Methods: In a sequence of physical, chemical and enzymatic decellularisation techniques mouse livers were stripped off all cellular components to obtain a 3D scaffold. HB cell lines were then seeded onto these scaffolds and cultivated for several weeks.

Results: Our newly generated biological scaffolds consist of liver-specific extracellular matrix components including collagen IV and fibronectin. A cultivation of HB cell lines on these scaffolds led to the formation of 3D tumour structures by infiltration into the matrix. Analyses of drug response to standard-of-care medication for HB showed reliable reproducibility of our stocked models.

Discussion: Our HB models are easy-to-handle, producible at large scale, and can be cryopreserved for ready-to-use on-demand application. Our newly generated 3D HB platform may therefore represent a faithful preclinical model for testing treatment response in precision cancer medicine.
An in vitro model of cancer invasion with heterogeneous ECM created with droplet microfluidics
Authors: Jouybar Mohammad , Sleeboom Jelle J. F. , Vaezzadeh Elnaz , Sahlgren Cecilia M. , den Toonder Jaap M. J.
Published: November 2023

Metastasis is a multi-step process that is critically affected by cues from the tumor micro-environment (TME), such as from the extracellular matrix (ECM). The role of the ECM in the onset of metastasis, invasion, is not yet fully understood. A further complicating factor is that the ECM in the TME is mostly heterogeneous, in particular presenting a basement membrane (BM) directly enveloping the tumor, which acts as a barrier to invasion into the surrounding stromal ECM. To systematically investigate the role of ECM in invasion, appropriate in vitro models with control over such ECM heterogeneity are essential. We present a novel high-throughput microfluidic approach to build such a model, which enables to capture the invasion of cancer cells from the tumor, through the BM and into the stromal tissue. We used a droplet-maker device to encapsulate cells in beads of a primary hydrogel mimicking BM, Matrigel, which were then embedded in a secondary hydrogel mimicking stromal ECM, collagen I. Our technology ultimately provides control over parameters such as tissue size, cell count and type, and ECM composition and stiffness. As a proof-of-principle, we carried out a comparative study with two breast cancer cell types, and we observed typical behavior consistent with previous studies. Highly invasive MDA-MB-231 cells showed single cell invasion behavior, whereas poorly invasive MCF-7 cells physically penetrated the surrounding matrix collectively. A comparative analysis conducted between our heterogeneous model and previous models employing a single type of hydrogel, either collagen I or Matrigel, has unveiled a substantial difference in terms of cancer cell invasion distance. Our in vitro model resembles an in vivo heterogeneous cancer microenvironment and can potentially be used for high throughput studies of cancer invasion.
Novel venous balloon for compliance measurement and stent sizing in a post-thrombotic swine model
Authors: Wang Mengjun , Lu Xiao , Han Ling , Wang Amy M. , Raju Seshadri , Kassab Ghassan S.
Published: November 2023

Objective: Real-time accurate venous lesion characterization is needed during endovenous interventions for stent deployment. The goal of this study is to validate a novel device for venoplasty sizing and compliance measurements.

Methods: A compliance measuring sizing balloon (CMSB) uses real-time electrical conductance measurements based on Ohm’s Law to measure the venous size and compliance in conjunction with pressure measurement. The sizing accuracy and repeatability of the CMSB system were performed with phantoms on the bench and in a swine model with an induced post thrombotic (PT) stenosis in the common femoral vein of swine.

Results: The accuracy and repeatability of the CMSB system were validated with phantom bench studies of known dimensions in the range of venous diameters. In 9 swine (6 experimental and 3 control animals), the luminal cross-sectional areas (CSA) increased heterogeneously along the PT stenosis when the CMSB system was inflated by stepwise pressures. The PT stenosis showed lower compliance compared to the non-PT vein segments (5 mm2 vs. 10 mm2 and 13 mm2 at a pressure change of 40 cm H2O). Compliance had no statistical difference between venous hypertension (VHT) and Control. Compliance at PT stenosis, however, was significantly smaller than that at Control and VHT (p < 0.05, ANOVA).

Conclusion: The CMSB system provides accurate, repeatable, real-time measurements of CSA and compliance for assessment of venous lesions to guide interventions. These findings provide the impetus for future first-in-human studies.
Targeting c-MYC: a potential non-hormonal therapeutic approach for endometriosis treatment
Authors: Nothnick Warren B. , Arachchige Sachith Polpitiya , Minchella Paige , Stephens Edward B. , Graham Amanda
Published: November 2023

Endometriosis is a benign gynecological disease in which eutopic endometrial tissue composed of glands and stroma grow within the pelvic cavity. The disease affects females of reproductive age and is characterized by pelvic pain, infertility and reduced quality of life. The majority of pharmacologic treatment modalities for endometriosis focus on suppression of estradiol production and/or action; an approach associated with adverse side effects. c-MYC is elevated in eutopic endometrium and endometriotic lesion tissue in patients with endometriosis and the disease shares many similar pathological characteristics with that of endometrial carcinoma. While targeting of c-MYC with Omomyc has recently gained substantial interest in the field of cancer research, there has been no recent attempt to evaluate the potential utility in targeting c-MYC for endometriosis treatment. The following perspective article compares the similarities between endometriosis and endometrial cancer and presents preliminary data suggesting that targeting c-MYC with Omomyc reduces endometriotic cell proliferation and viability in vitro. Future application of targeting c-MYC in endometriosis treatment and potential pros and cons are then discussed.
Quercetin improves and protects Calu-3 airway epithelial barrier function
Authors: DiGuilio K. M. , Rybakovsky E. , Valenzano M. C. , Nguyen H. H. , Del Rio E. A. , Newberry E. , Spadea R. , Mullin J. M.
Published: November 2023

Introduction: In light of the impact of airway barrier leaks in COVID-19 and the significance of vitamin D in COVID-19 outcomes, including airway barrier protection, we investigated whether the very common dietary flavonoid quercetin could also be efficacious in supporting airway barrier function.

Methods: To address this question, we utilized the widely used airway epithelial cell culture model, Calu-3.

Results: We observed that treating Calu-3 cell layers with quercetin increased transepithelial electrical resistance while simultaneously reducing transepithelial leaks of 14C-D-mannitol (Jm) and 14C-inulin. The effects of quercetin were concentration-dependent and exhibited a biphasic time course. These effects of quercetin occurred with changes in tight junctional protein composition as well as a partial inhibition of cell replication that resulted in decreased linear junctional density. Both of these effects potentially contribute to improved barrier function. Quercetin was equally effective in reducing the barrier compromise caused by the pro-inflammatory cytokine TNF-α, an action that seemed to derive, in part, from reducing the elevation of ERK 1/2 caused by TNF-α.

Discussion: Quercetin improved Calu-3 barrier function and reduced TNF-α-induced barrier compromise, mediated in part by changes in the tight junctional complex.
Establishment of the mechanism of purification and levigation of green chemistry-assisted biocomposites of red ochre (Gairika): synthesis, characterization, and antibacterial, prebiotic, antioxidant, and antacid activities of the traditional Ayurvedic medicine Laghu Sutashekhara Rasa
Authors: Charde Vaibhav , Kumar Vijay , Dane Ganesh , Gandhi Yashika , Soni Hemant , Jagtap Chandrashekar , Mishra Sujeet K. , Shakya Santosh K. , Singh Arjun , Singh Ravindra , Sharma Bhagwan S. , Khanduri Shruti , Srikanth Narayanam , Acharya Rabinarayan , Webster Thomas J.
Published: November 2023

Gairika (red ochre) has a long history of influencing human civilization. Gairika is a rich source of nutrients used for reproductive and brain health. Gairika is mentioned as an antacid drug in Indian Ayurvedic medicine under Laghu Sutashekhara Rasa (LSR). However, a detailed study on LSR has not been reported to date. In the present study, LSR was prepared, and a pharmaceutical SOP (standardization procedure) was reported to obtain batch-to-batch reproducibility. LSR was characterized using FTIR, XRD, SEM-EDX, and TGA analyses. LSR was tested in vitro for its antacid activity. Advanced instrumentation revealed that LSR formation produced symmetrical particles (5–8 µm) with kaolin, kaolinite, quartz, goethite, and hematite, along with the phytoconstituents of Goghrita (clarified cow’s butter), Shunthi, and Nagawalli, as confirmed by GC-MS/MS analysis. The FTIR study revealed the formation of a chelating complex of goethite and hematite along with their phytoconstituents. XRD analysis confirmed the presence of kaolin, kaolinite, quartz, goethite, and hematite. Using in vitro antacid experiments, LSR and Shunthi demonstrated significant antacid activity as compared to antacid drugs and standards in the market, such as CaCO3. The DPPH assay revealed IC50 values of 12.16 ± 1.23 mg/mL, which is 0.0029 of Trolox-equivalent antioxidant activity. The inhibition (18 ± 4 mm) against pathogens (S. aureus, E. coli, P. aeruginosa, and B. subtilis) and the prominent growth of gut microbiota-supported strains (S. boulardii, L. paracasei, and L. plantarum) observed on LSR formulation were indicative of LSR application as a prebiotic. Here, the mechanism of purification and levigation mentioned in the classical literature of LSR was established. Overall, purification of Gairika with cow ghee and levigation with Nagawalli may enhance the solubility, bioavailability, and shelf-life of LSR through hydration and co-crystallization mechanisms. This is the first comprehensive report on the pharmaceutical validation of LSR and its characterization. The results of the present study could contribute to the development and reliable reproduction of LSR and the utility of environmental red ochre as a medicine in combination with Shunthi (Zingiber officinale Roxb.), as prescribed under Indian Ayurvedic medicine.
Association between the variability of non-high-density lipoprotein cholesterol and the neutrophil-to-lymphocyte ratio in patients with coronary heart disease
Authors: Chen Yifan , Zhang Sisi , Tao Yecheng , Hu Wenyi , Li Duanbin , Shen Xiaohua , Li Ya , Lin Maoning , Zhang Wenbin , Liu Xianglan , Xie DaQi
Published: November 2023

Background

Lowering lipid variability may be a potential strategy for improving the inflammatory state in patients with coronary heart disease (CHD). This study investigated the association between the variability of non-high-density lipoprotein cholesterol (non-HDL-C) and the neutrophil-to-lymphocyte ratio (NLR).

Methods

This study enrolled 2,711 CHD patients subjected to percutaneous coronary intervention (PCI). During the 1-year follow-up period after PCI, the variability of non-HDL-C was assessed using standard deviation (SD), coefficient of variation (CV), and variability independent of mean (VIM). NLR was calculated as the ratio of absolute neutrophil count to absolute lymphocyte count. The relationship between the non-HDL-C variability and the average NLR level during follow-ups was examined using a linear regression analysis.

Results

The mean age of the patients was 64.4 ± 10.8 years, with 72.4% being male. The average NLR level was 2.98 (2.26–4.14) during the follow-up (1 year after PCI). The variability of non-HDL-C was 0.42 (0.26–0.67) for SD, 0.17 (0.11–0.25) for CV, and 0.02 (0.01–0.03) for VIM. A locally weighted scatterplot smoothing curve indicates that the average levels of NLR increased with increasing variability of non-HDL-C. Regardless of the variability assessment method used, non-HDL-C variability was significantly positively associated with the average NLR level during follow-ups: SD [β (95% CI) = 0.681 (0.366–0.996)], CV [β (95% CI) = 2.328 (1.458–3.197)], and VIM [β (95% CI) = 17.124 (10.532–23.715)]. This association remained consistent across subgroups stratified by age, gender, diabetes, and hypertension.

Conclusion

The variability of non-HDL-C was positively associated with NLR in patients with CHD, suggesting that reducing non-HDL-C variability may improve the low-grade inflammatory state in CHD patients.
The association between EPCR gene p.Ser219Gly polymorphism and venous thromboembolism risk: a case–control study, meta-analysis, and a reproducibility study
Authors: Pituk Dóra , Miklós Tünde , Schlammadinger Ágota , Rázsó Katalin , Bereczky Zsuzsanna
Published: November 2023

Background

The rs867186 single-nucleotide polymorphism in the PROCR gene (g.6936A > G, c.4600A > G) results in a serine-to-glycine substitution at codon 219 of endothelial protein C receptor (EPCR). We performed a case–control study followed by an updated meta-analysis of the association between this polymorphism and the risk of venous thromboembolism (VTE).

Objective and methods

We enrolled 263 VTE patients and 320 unrelated healthy controls for the case–control study. The total number of cases and controls for the meta-analysis were 5,768 and 30,017, respectively. A new online MetaGenyo Statistical Analysis System software was used to perform the current meta-analysis. Furthermore, a reproducibility study was conducted to validate our results.

Results

Among well-defined thrombosis risk factors, Factor V Leiden was more frequent in the VTE group (p < 0.001), while there was no difference in mutation frequency of prothrombin 20210G>A polymorphism between the two groups. There was no difference in the mutation frequency of Factor V Leiden and prothrombin 20210G>A between cases with and without provoking factors and cases with and without VTE recurrence. The rs867186 “G” carriership did not influence the risk of VTE [odds ratio (OR) 1.339; 95% confidence interval (CI): 0.904–1.984] in our study. No significant differences could be demonstrated among the rs867186 genotype frequencies between VTE cases with and without provoking factors (p = 0.430). PROCR rs867186 was associated with an OR of 1.72 (95% CI: 0.95–3.13, p = 0.075) in terms of VTE recurrence. In the meta-analysis, a significant association was found between EPCR Ser219Gly polymorphism and VTE under the dominant model (OR = 1.27, 95% CI: 1.11–1.46, p = 0.0006), the recessive model (OR = 1.60, 95% CI: 1.26–2.04, p = 0.0001), the GG vs. AA contrast model (OR = 1.64, 95% CI: 1.28–2.09, p = 0.0001), and the GA vs. AA contrast model (OR = 1.24, 95% CI: 1.08–1.43, p = 0.002).

Conclusion

The rs867186 was not associated with the first VTE risk in our case–control study; however, a tendency to VTE recurrence was observed. Based on the results of our reproducibility study, MetaGenyo is acceptable for meta-analysis in case of genetic epidemiology studies. Although the risk conferred by the rs867186 is mild in all meta-analyses, including ours, identifying patients carrying the minor allele might have an impact on personalized VTE risk assessment, risk-score calculation, and patient management.