bluuebunny/crossref_metadata_2025 huggingface dataset:
A Scoring System to Predict Difficult Laparoscopic Cholecystectomy: A Five-Year Cross-Sectional Study
Authors: Ary Wibowo Agung , Tri Joko Putra Oscar , Noor Helmi Zairin , Poerwosusanta Hery , Kelono Utomo Tjahyo , Marwan Sikumbang Kenanga
Published: September 2022

Background. Laparoscopic cholecystectomy since long time already has become the preferred method because laparoscopic cholecystectomy has many advantages compared to standard open cholecystectomy. However, since it has associated with a higher risk of complication, preoperative prediction of risk factors is needed to assess the intraoperative difficulties. Various scoring systems have a role in predicting intraoperative difficulties; however, there is a need to find a consistent and reliable predictive system. Aim. To validate a preoperative scoring system that will predict difficult laparoscopic cholecystectomy. Design of the Study. Nonrandomized retrospective descriptive study. Setting. Department of General Surgery, Lambung Mangkurat Univeristy Ulin Referral Hospital, Banjarmasin, Kalimantan Selatan, Indonesia. Methodology. A preoperative score was given to all the patients (134 patients from January 2015–December 2020) based on history, clinical examination, and sonographic findings. Using ROC curve, the cutoff for easy—difficult was 3.5 and difficult—very difficult was 7.5. The scores were compared in each patient to conclude the practicality of the preoperative predictive score. SPSS version 25 was used to analyze the data. Results. History of hospitalization for acute cholecystitis (p≤0.001), high BMI (p=0.002), abdominal scar (p=0.005), palpable gallbladder (p≤0.001), thick gallbladder wall (p≤0.001), and leucocyte (p≤0.001) were considered as the significant factors that predict difficult laparoscopic cholecystectomy. Sensitivity and specificity for easy—difficult cutoff of the scoring method were 72.6% and 87.5%, respectively, with the area under the ROC curve being 0.849. Sensitivity and specificity for difficult—very difficult cutoff of the scoring method were 70.0% and 84.5%, respectively, with the area under the ROC curve being 0.779. Conclusion. The preoperative scoring system evaluated in the study is reliable and beneficial in predicting the difficulty of laparoscopic cholecystectomy. However, further randomized prospective multicentric studies with large sample sizes are required to validate the efficiency of the scoring system.
Separating Mixture Glycols in DWC with Flow Rate-Composition Cascade Control Structure
Authors: Ma Zhihui , Wu Yanyang , Xu Jianyuan , Chen Kui , Wu Bin , Ji Lijun
Published: September 2022

Dividing wall column (DWC) is an advanced technology which could decrease energy consumption. As the vertical wall inserted in DWC increases the degree of freedom of systems, the control becomes more difficult than the conventional columns. In this study, the mixture glycols (1, 2-propanediol+ 1, 3-butanediol+ 1, 4-butanediol) were taken as feed, an optimized DWC structure was proposed, and three control structures for DWC were tested by ± 10% feed disturbances. The temperature control structure (CS1) was hard to control the system while composition control structure (CS2) and flow rate-composition cascade control structure (CS3) had good performance on controlling the DWC. It showed that CS3 was superior to CS2, as CS3 could stabilize the DWC within 4 hours, and the maximum deviations of the three products, namely 1, 2-propanediol, 1, 3-butanediol and 1, 4-butanediol, were 0.07%, 1.44%, and 0.46% respectively, while CS2 could realize the stability within 7 hours, and the corresponding numbers were 0.35%, 2.78%, and 0.41%, respectively.
Green Message Framing in Enhancing Sustainable Consumption Behavior of Fashion Based on the Cross‐Theoretical Model
Authors: Chen Lihong , He Lin , Yan Xinfeng , Liu Chunhong
Published: September 2022

Promoting green consumption is key in meeting ambitious sustainable fashion targets being set around the world. This research examined how framing of green message as positive or negative (i.e., benefit framing) influenced formation of sustainable consumption behaviors of fashion (SCBF) based on the cross‐theoretical model and, especially, how self‐efficacy, decision balancing, and perceiving threats‐mediated green message framing effects. Data were collected from 217 Chinese residents in an online experiment. Our findings show that green message framing has different effects on individuals in different change stages of SCBF and loss framing‐based green messages induce more positive responses toward SCBF with greater perceived threats in the pre‐intention and intention stages, while gain framing‐based green messages might stimulate positive behaviors toward SCBF with greater perceived benefits in the preparation and action and maintenance stages. Results suggest that highlighting green message expression in relating to SCBF may be useful for promoting broader sustainable behaviors. Therefore, this article significantly fills the gaps between green message framing and SCBF. The findings of this article have significant implications for fashion companies who wish to explore the fashion green market potential.
The Role of N6-Methyladenosine-Associated lncRNAs in the Immune Microenvironment and Prognosis of Colorectal Cancer
Authors: Yuan Congfei , Liu Caidong , Zhao Shuli , Zhang Xishan , Jia Haifeng , Chen Baiyu , Zhang Maojin , Zheng Yuan , Zhou Jin , Bo Yanzhi
Published: September 2022

Background. The role of N6-methyladenosine long noncoding RNAs (lncRNAs) in colorectal cancer (CRC) is elusive. Materials and Methods. We identified m6A-associated lncRNAs by using the data gathered from The Cancer Genome Atlas (TCGA) and stratified CRC patients into different subgroups. Cox regression analysis was performed to construct an m6A-associated lncRNA signature. The role of this signature in the immune microenvironment and prognosis was dissected subsequently. Finally, a gene set enrichment analysis (GSEA) was conducted to predict the possible mechanisms based on the signature. Results. Three m6A-associated clusters were constructed from 866 differentially expressed lncRNAs. Cluster 2 had poor prognosis and low immune cell infiltration. An m6A-associated lncRNA signature consisting of 14 lncRNAs was constructed and recognized as an independent prognostic indicator of CRC by using survival analysis and receiver operating characteristic (ROC) curves. The clinical features and immune cell infiltration status were significantly different in patients stratified by the risk score. Furthermore, GSEA showed that the P53 pathway and natural killer cell-mediated cytotoxicity were more enriched in the low-risk group. Conclusion. Our data revealed that m6A-associated lncRNAs could be potential prognostic indicators of immunogenicity in CRC.
From Spatial to Spectral Domain, a New Perspective for Detecting Adversarial Examples
Authors: Liu Zhiyuan , Cao Chunjie , Tao Fangjian , Li Yifan , Lin Xiaoyu
Published: September 2022

Deep neural networks (DNNs) have been closely related to the Pandora’s box from the moment of its birth. Although it achieves a high accuracy significantly in real-world tasks (e.g., object detecting and speech recognition), it still retains fatal vulnerabilities and flaws. Malicious attackers can manipulate DNN model misclassification just by adding tiny perturbations to the original image. These crafted samples are also called adversarial examples. One of the effective defense methods is to detect them before feeding them into the model. In this paper, we delve into the representation of adversarial examples in the original spatial and spectral domains. By qualitative and quantitative analysis, it is confirmed that the high-level representation and high-frequency components of abnormal samples contain richer discriminative information. To further explore the influence mechanism between the two factors, we perform an ablation study and the results show a win-win effect. Utilizing the finding, a detecting method (HLFD) is proposed based on extracting high-level representation and high-frequency components. Compared with other state-of-the-art detection methods, we achieve a better detection performance in most scenarios via a series of experiments conducted on MNIST, CIFAR-10, CIFAR-100, SVHN, and Tiny-ImageNet. In particular, we improve detection rates by a large margin on DeepFool and CW attacks.
Numerical Simulation Study on the Development Effect of Gravity Fire Flooding by Vertical Well Sidetracking
Authors: Yuan Shibao , Song Jia , Li Lehong , Jiang Haiyan , Sun Xinge
Published: September 2022

As a development method to replace steam stimulation of heavy oil, in situ combustion often faces many problems in thick reservoir, such as low vertical sweep efficiency and channeling combustion. According to the characteristics and development history of this kind of reservoir, a method of changing plane fire flooding to gravity fire flooding is proposed by using the existing steam stimulation development well pattern and sidetracking horizontal section of vertical production wells. The influence of different factors on sidetracking gravity fire flooding production effect is analyzed from the aspects of reservoir geology and development engineering. The internal stimulation mechanism of this method is further studied, and the evaluation model between recovery factor and main control factor is established by using multiple linear regression equation. The results show that vertical sidetracking gravity fire flooding can improve the recovery of thick heavy oil reservoir by 42%; the better recovery effect can be obtained when the sidetracking length is about 1/2 of the well spacing; the coincidence degree between the established recovery evaluation model and the results of numerical simulation is more than 85%. The research results of this paper can help the mine fire flooding development to change the mining mode and provide some guidance for the medium- and long-term planning.
Mobile Learning New Trends in Emerging Computing Paradigms: An Analytical Approach Seeking Performance Efficiency
Authors: Mohiuddin Khalid , Miladi Mohamed Nadhmi , Ali Khan Mohiuddin , Khaleel Mohammad Abdul , Ali Khan Sajid , Shahwar Samreen , Nasr Osman A. , Aminul Islam Mohammad
Published: September 2022

Mobile learning (m-learning) adoption has increased and shall be demonstrated superior performance by implementing related computing paradigms, such as IoT, edge, mobile edge, fog, AI, and 5G. Mobile cloud architectures (MCAs) enable m-learning with several benefits and face limitations while executing real-time applications. This study investigates the state-of-the-art m-learning architectures, determines a layered m-learning-MCA obtaining numerous benefits of related computing paradigms, and expands m-learning functional structure. It evaluates m-learning performance across the four physical layer’s MCAs—distance cloud, cloudlet, operator-centric cloud, ad hoc cloud, and emerging computing architectures. Surprisingly, only distance-cloud MCA is adopted for developing m-learning systems by ignoring the other three. Performance evaluation shows m-learning gets terrific benefits and users QoE in related computing paradigms. Mobile edge computing offers ultralow latency, whereas the current architecture improves task execution time (1.87, 2.01, 2.63, and 3.97) for the resource-intensive application (i.e., 4.2 MB). Fog using AI algorithms is exceptional for more complex learning objects, IoT is superior for intelligent learning tools, and 5G ultrawideband services are more significant for intelligent video analytics. These findings help learners, educators, and institutions adopt an appropriate model for achieving their academic objectives across educational disciplines. The presented approach enables future research to design innovative architectures considering resource-intensive m-learning application execution requirements, such as video content analytics and virtual reality learning models.
Hybrid Genetic Algorithm for IOMT-Cloud Task Scheduling
Authors: Hussain Adedoyin A. , Al-Turjman Fadi
Published: September 2022

Task scheduling for the cloud is one of the main advances in IoMT stage, which impacts the whole execution of the cloud resource. Cloud is a proficient headway for computation, and it encompasses data storage, management, and manipulation in large volumes. Thus, a proposition is being made a better approach to proffer task scheduling in the cloud. In this case, a new hybrid genetic algorithm (HGA) is proposed. The proposed HGA method will be justified by contrasting it with the previous researches and approaches. The CloudSim is utilized to quantify their effect on various metrics like timing factors and resource utilization. The proposed HGA technique enhanced the viability of task scheduling with a better execution rate of 32.57 ms. Thus, the experimented outcomes show that the HGA also reduces cost profoundly.
A Sub‐Supersolution Method for p‐Laplacian Equation with Non‐Local Term
Authors: Rong Mei , Miao Qing
Published: September 2022

This paper is concerned with the existence of solutions for p‐Laplace problems with non‐local term. We prove the sub‐supersolution theorem using the pseudomonotone operator theorem and Minty–Browder theorem with appropriate assumptions on M, gi(i = 1,2). Then, using the sub‐supersolution approach, we determine the existence of positive solutions to specific problems.
Response to Direct Selection against Drought Stress in Black Cumin (Nigella sativa L.)
Authors: Kayacetin Fatma
Published: September 2022

Central Anatolia is prone to drought with low precipitation and high evapotranspiration which influence the growth of a several crops. The selection and cultivation of drought-tolerant crops that can survive and maintain under poor climatic conditions are very important. Black cumin (Nigella sativa L. Umbelliferae) is famous for aromatic, medicinal, and therapeutic uses in the Mediterranean region and elsewhere. Drought stress most often delays or reduces seed germination ending up in irregular and poor crop growth. The study aimed to compare the effects of −0.1, −0.2, and −0.4 MPa of PEG 6000 osmo-priming pretreatments for 12, 24, and 36 h and two PEG 6000 post-treatments of −0.05 and −0.15 MPa along with their respective controls for 14 days as post-treatment on seed germination and seedling establishment potential of Turkish cv. Cameli. The results showed significant differences among germination index, germination stresses tolerance index, germination speed, final germination percentage, seedling vigor index, mean germination time, shoot, root and seedling length, and seedling fresh and dry weight of pretreatments and post-treatments when compared to nonprimed and hydro-primed seeds used as control treatments. Osmo-priming pretreatments of black cumin using −0.2 or −0.4 MPa PEG 6000 for 24 or 36 hours in a medium containing −0.05 MPa PEG 6000 as post-treatment showed improved germination efficiency, with increased adaptation ability.
Multitech-Based Study on Medicinal Material Basis and Action Mechanism of Herbal Formula Xian-Ling-Gu-Bao Capsule in Treatment of Osteoarthritis
Authors: Wu Xiaowen , Sun Shuai , Wu Xiaoyi , Sun Zengxian
Published: September 2022

Currently, osteoarthritis (OA) is thought to be the most prevalent chronic joint disease worldwide. The epidemiology of this disorder is complex, and the treatment is challenging. Xian-Ling-Gu-Bao (XLGB) capsule, a herbal compound preparation, is widely used for the treatment of bone disorders, including OA. Although its efficacy and safety have been demonstrated in clinical trials and practice, the underlying medicinal constituents and mechanism have not been clearly elucidated. Therefore, this study aimed to explore the medicinal constituents and mechanism of XLGB for OA treatment. The phytochemical constituents in XLGB capsule were detected by liquid chromatography-mass spectrometry (LC-MS), the medicinal constituents and therapeutic mechanism for OA treatment were deduced by network analysis, and the deduced mechanism was validated by in vitro experiment. As a result, a total of 55 constituents were detected in XLGB extract, in which 16 constituents were screened out for target collection. Based on the analysis of target profile, XLGB targets showed a high degree of similarity with OA targets. Network analysis revealed that XLGB had a holistic effect of multiple active constituents on multiple targets and pathways. The core targets of XLGB were presumed to be MAPKs, PI3K, AKT, BCL2, RELA, TNF, NOS2, and so on, and the mechanism was speculated to mainly inhibit chondrocyte apoptosis and inflammatory response through JNK and PI3K/AKT/NF-κB signaling cascades. Finally, in vitro study confirmed that XLGB extract protected ATDC5 cells against lipopolysaccharide- (LPS-) induced apoptosis and inflammatory response, and these effects were supposed to be involved in the inhibition of JNK and PI3K/AKT/NF-κB pathways. Our study could provide a scientific basis for further research and clinical use of XLGB capsule.
Recurrent Episodes of Dissociative Fugue with Comorbid Severe Depression and Alcohol Dependence Syndrome
Authors: Daliri Dennis Bomansang , Afaya Agani , Koomson William H. F. , Akatibo Emmanuel
Published: September 2022

The rarity and close resemblance to other mental health conditions of dissociative fugue make it difficult to diagnose. Akin to a culture-bound syndrome, most African countries have their local explanation for this unique presentation and therefore people may not seek evidence-based health care but rather may resort to faith-based-treatment which may not give the best results. This is the case of a 39-year-old man who has experienced about five episodes of dissociative fugue over nine years. This case points out the comorbidities associated with the condition. We report this case to increase awareness of the condition and to bring to the fore the need for further studies into psychopathology and the treatment modalities.
Molecular Mechanism of YuPingFeng in the Treatment of Asthma Based on Network Pharmacology and Molecular Docking Technology
Authors: Shen Li , Lu Jinmiao , Wang Guangfei , Wang Cheng , Li Zhiping
Published: September 2022

Objective. To explore the molecular targets and mechanism of YuPingFeng (YPF) for the treatment of asthma by using network pharmacology and molecular docking. Methods. The potential active ingredients and relevant targets of YPF were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). Asthma-related gene targets were retrieved from GeneCards, OMIM, DrugBank, PharmGKB, and TTD databases. The protein-protein (PPI) network between YPF and asthma common targets was constructed by SRING online database and Cytoscape software. GO and KEGG analyses were performed to explore the complicated molecular biological processes and potential pathways. Finally, a molecular docking approach was carried out to verify the results. Results. We obtained 100 potential targets of the 35 active ingredients in YPF and 1610 asthma-related targets. 60 YPF-asthma common targets were selected to perform PPI analysis. Seven core genes were screened based on two topological calculation methods. GO and KEGG results showed that the main pathways of YPF in treating asthma include TNF signaling pathway and PI3K-Akt signaling pathway. Finally, the molecular docking results indicated that the key ingredients of YPF had a good affinity with the relevant core genes. Conclusion. This study reflects the multicomponent, multitarget, and multipathway characteristics of YPF in treating asthma, providing a theoretical and scientific basis for the intervention of asthma by traditional Chinese medicine YPF.
Is There Any Relationship between Trichomonas vaginalis Infection and Male Urethritis Risk? A Systematic Review and Meta-Analysis
Authors: Ziaei Hezarjaribi Hajar , Saberi Reza , Fakhar Mahdi , Sadeghian Najmeh
Published: September 2022

Background. Male urethritis is one of the most common genital tract syndromes. Though the number of patients with urethritis is increasing worldwide, the cause of many cases of non-gonococcal urethritis (NGU) is still unknown. Objectives. This study aimed to delineate the association between Trichomonas vaginalis (T. vaginalis) infection and male urethritis. Methods. The literature was searched in PubMed, Scopus, and Web of Science databases using the search terms “urethritis,” “Trichomonas vaginalis,” “trichomoniasis,” and “male urethritis” up to February 2020. Overall risk difference(RD) was applied to assess the relationship between T. vaginalis infection and male urethritis. Results. In total, seven articles were included in this systematic review and meta-analysis study. Our meta-analysis involved the review of case-control studies, including 2,242 urethritis cases and 929 individuals as controls. Among subjects examined for trichomoniasis, in the case group, 211 males were infected, and in the control group, 32 individuals were infected. The overall risk difference (RD) was 0.06, and the total reported

p

value was 0.00001. Although the result of our meta-analysis was not significant, it was shown that the risk of urethritis is 0.06 more in trichomoniasis patients than in the non-exposed group. Conclusion. Findings from the included papers showed that trichomoniasis is not a risk factor for male urethritis. Although trichomoniasis alone is not the main cause of urethritis, it can be considered one of the risk factors in male urethritis. Therefore, in the future, it is necessary to perform further studies to clarify the detailed association between T. vaginalis infection and urethritis risk in male patients.
Energy and Path-Aware-Reliable Routing in Underwater Acoustic Wireless Sensor Networks
Authors: Ali Munsif , Khan Anwar , Bertozzi Massimo , Ullah Ubaid , Altowaijri Saleh M. , Ali Ihsan , Iqbal Salman
Published: September 2022

In underwater acoustic sensor networks (UASNs), energy awareness, best path selection, reliability, and scalability are among the key factors that decide information delivery to the sea surface. Existing protocols usually do not combine such performance-affecting factors in information routing. As a result, the performance of such protocols usually deteriorates if multiple performance factors are taken into account. To cope with such performance deterioration, this article proposes two routing protocols for UASNs: energy and path-aware reliable routing (EPRR) and cooperative EPRR (Co-EPRR). Compared with the counterpart systems, the proposed protocols have been designed to deal with the problem of long propagation delays and achieve network reliability. The EPRR scheme uses nodes’ physical distance from the surface with its depth, which minimized the delay of packet transmission. The channel interaction time has been reduced, therefore, reducing unwanted channel effects on the data. Furthermore, the density of the nodes in the upper part of the network prevents data loss and limits the rapid death of the nodes. The second proposed scheme, Co-EPRR, uses the concept of routing information from the source to the destination on multiple paths. In Co-EPRR routing, the destination node can receive more than one copy of the data packet. This reduces unfavorable channel effects during data delivery. Both the schemes show good performance in terms of packet delivery ratio, received packet analysis, and end-to-end delay.
Purification and Analysis of the CREPT Antibody from Mouse Ascites
Authors: Xie Zhihao , Li Jun , Hao Xinbao , Xu Lu
Published: September 2022

Background and objective. The cell cycle-related and expression-elevated protein in tumor (CREPT) is overexpressed in several human cancers. Establishing a method for purifying CREPT from mouse ascites is vital for further research. This study was aimed at establishing a method for purifying CREPT from mouse ascites. Methods. Cells were cultivated properly to obtain 3E10 CREPT monoclonal antibody cells in the logarithmic growth stage. Monoclonal antibody cells were injected into the abdominal cavity of sensitized mice. The flowing ascites were observed for 7-15 days. The antibody protein was obtained by collection, filtration, dilution, loading, and chromatography. Furthermore, its binding force was detected by SDS-PAGE and Western blot techniques. Results. The antibody protein was successfully obtained with a purity of 1895 μg/mL with high liveness. Conclusion. This study establishes a one-step purification method for obtaining monoclonal antibody with high liveness and purity for CREPT ascites antibody. This method is simple to perform and lays a foundation for the preparation and purification of humanized monoclonal antibodies in the future. In addition, it provides a basis for further research to investigate how CREPT affects the occurrence and development of different tumors.
A Potential Anticancer Mechanism of Finger Root (Boesenbergia rotunda) Extracts against a Breast Cancer Cell Line
Authors: Widyananda Muhammad Hermawan , Wicaksono Septian Tri , Rahmawati Kurnia , Puspitarini Sapti , Ulfa Siti Mariyah , Jatmiko Yoga Dwi , Masruri Masruri , Widodo Nashi
Published: September 2022

Breast cancer is the most common type of cancer women suffer from worldwide in 2020 and the 4th leading cause of cancer death. Boesenbergia rotunda is an herb with high potential as an anticancer agent. This study explores the potential bioactive compounds in B. rotunda as anti-breast cancer agents using in silico and in vitro approaches. The in silico study was used for active compound analysis, selection of anticancer compound candidates, prediction of target protein, functional annotation, molecular docking, and molecular dynamics simulation, respectively. The in vitro study was conducted by measurement toxicity, rhodamine 123, and apoptosis assays on T47D cells. Based on the KNApSAcK database, B. rotunda contained 20 metabolites, which are dominated by chalcone and flavonoid groups. Seven of them were predicted to have anticancer activity, namely, sakuranetin, cardamonin, alpinetin, 2S-pinocembrin, 7.4′-dihydroxy-5-methoxyflavanone, 5.6-dehydrokawain, and pinostrobin chalcone. These compounds targeted proteins related to cancer progression pathways such as the PI3K/Akt, FOXO, JAK/STAT, and estrogen signaling pathways. Therefore, these compounds are predicted to inhibit growth and induce apoptosis of cancer cells through their interactions with MMP12, MMP13, CDK4, JAK3, VEGFR1, VEGFR2, and KCNA3. Anticancer activity of B. rotunda through in vitro study confirmed that B. rotunda extract is strong cytotoxic and induces apoptosis of breast cancer cell lines. This study concludes that Boesenbergia rotunda has potency as an anticancer candidate.
Novel Method of Analysis for the Determination of Residual Formaldehyde by High-Performance Liquid Chromatography
Authors: Delbono Vittoria , Larch Christopher P. , Newlands Katrina Carol , Rhydderch Shona , Baddeley Thomas Craven , Storey John Mervyn David
Published: September 2022

Formaldehyde is commonly used as an alkylating agent in the pharmaceutical industry. Consequently, its residual level in drug substances and/or their intermediates needs to be accurately quantified. Formaldehyde is a small, volatile molecule with a weak chromophore (the carbonyl group), and its direct analysis by GC-FID and HPLC-UV is difficult. For these reasons, the majority of papers found in the literature are based upon a derivatisation process (most commonly using the desensitised explosive 2,4-dinitrophenylhydrazine) prior to the analysis of formaldehyde. A novel high-performance liquid chromatography (HPLC) method with UV detection for its quantification in a pharmaceutical is described in this paper. The method proposed herein is based upon a derivatisation reaction between formaldehyde and 4-methylbenzenesulfonohydrazide (MBSH) before analysis by HPLC-UV. Selectivity, linearity, limit of quantification, accuracy, repeatability, intermediate precision, and solution stability were successfully assessed as per ICH guideline Q2(R1), and the method has also been validated in a good manufacturing practice (GMP) laboratory in the UK.
Ecological Integrity Evaluation of Organically Evolved Cultural Landscape
Authors: Xu Wenting
Published: September 2022

When the traditional method is used to evaluate the cultural landscape as a whole, due to the lack of historical and cultural factors, the impact of the ecological environment on the landscape change is not considered. Therefore, it cannot objectively reflect the law of landscape change. Therefore, from the perspective of the combination of cultural landscape composition characteristics and cultural ecological characteristics, this paper puts forward the concept of cultural ecological integrity based on the existing concept of cultural heritage integrity, aiming at the organic evolution of cultural landscape in cultural landscape heritage, and expounded the dynamic sustainability and effectiveness of natural environment and social environment on the process of cultural landscape. Considering the relevant characteristics and different forms of cultural landscape and cultural ecological integrity, according to the concept of cultural ecological integrity, this paper gave the principles and standards of cultural landscape ecological integrity evaluation and put forward the evaluation method of cultural ecological integrity. At the same time, each index in the ecological integrity evaluation model was decomposed and explained. Finally, taking the cultural heritage of Long-men Grottoes as an example, the paper used the cultural and ecological integrity evaluation model proposed in this paper to evaluate the material cultural landscape, intangible cultural landscape, and local traditional dwellings of Long-men Grottoes and made a comparative analysis between the control group and the observation group. The results showed that the cultural landscape ecological integrity evaluation model proposed in this paper can not only objectively evaluate the cultural landscape but also provide a certain theoretical and practical reference for the effective classification, protection and sustainable development, and utilization of the subsequent cultural landscape.
An Integrated Multiattribute Group Decision-Making Approach for Risk Assessment in Aviation Emergency Rescue
Authors: Wang Xia , Liang Xue-dong , Li Xiao-yan , Luo Peng
Published: September 2022

An effective risk assessment approach for assessing the risk level of aviation emergency rescue is essential to aviation emergency management. Therefore, this study is aimed at extending a novel integrated multiattribute group decision-making (MAGDM) approach to conduct risk assessment in aviation emergency rescue. First, a combined improved Swiss cheese model and fault tree analysis method are used to build the index system for risk assessment. Next, the weight vector for risk assessment is calculated using the consensus-based improved analytic hierarchy process (IAHP). Then, based on the traditional technique for order preference by similarity to the ideal solution (TOPSIS) method, we improve the definition express of ideal solution and the standardized formula of indexes, thereby providing a consensus-based improved TOPSIS method to compute the evaluation matrix for risk assessment. Finally, the weight vector and evaluation matrix are combined to estimate the risk level of aviation emergency rescue. As aviation emergency rescue in a sudden natural disaster an example, we illustrate the feasibility of the proposed method and demonstrate its effectiveness by comparative analysis with some similar approaches. The proposed approach can evaluate the safety status of aviation emergency rescue operations, which provides a starting point for the improvement of aviation emergency rescue.
Sleep Patterns and Risk of Prostate Cancer: A Population-Based Case Control Study in France (EPICAP)
Authors: Cordina-Duverger Emilie , Cénée Sylvie , Trétarre Brigitte , Rebillard Xavier , Lamy Pierre-Jean , Wendeu-Foyet Gaelle , Menegaux Florence
Published: September 2022

Background:

Sleep disturbances have been singled out for their implication in the risk of several cancer sites. However, results for prostate cancer are still inconsistent.

Methods:

We used data from the EPICAP study, a French population-based case–control study including 819 incident prostate cancer cases and 879 controls frequency matched by age. Detailed information on sleep duration on work/free days, and sleep medication over lifetime was collected.

Results:

Sleep duration and sleep deprivation were not associated with prostate cancer, whatever the aggressiveness of prostate cancer. However, sleep deprivation was associated with an increased prostate cancer risk among men with an evening chronotype [OR, 1.96; 95% confidence interval (CI), 1.04–3.70]. We also observed an increased risk of prostate cancer with higher duration of sleep medication use (Ptrend = 0.008). This association with long duration of sleep medication use (≥10 years) was more pronounced among men who worked at night 15 years or more (OR, 3.84; 95% CI, 1.30–11.4) and among nonusers of NSAID (OR, 2.08; 95% CI, 1.15–3.75).

Conclusions:

Our results suggested that chronotype, night work, or NSAID use could modify the association between sleep disorders and prostate cancer occurrence needing further investigations to go further.

Impact:

EPICAP is the first study, which investigates several sleep indicators taking into account potential effect modifiers. If our findings were confirmed, we could identify subgroups of men at higher risk of prostate cancer that may be accessible to preventive measures.
Plasma CD27, a Surrogate of the Intratumoral CD27–CD70 Interaction, Correlates with Immunotherapy Resistance in Renal Cell Carcinoma
Authors: Benhamouda Nadine , Sam Ikuan , Epaillard Nicolas , Gey Alain , Phan Letuan , Pham Hang Phuong , Gruel Nadège , Saldmann Antonin , Pineau Joséphine , Hasan Milena , Quiniou Valentin , Nevoret Camille , Verkarre Virginie , Libri Valentina , Mella Sebastien , Granier Clémence , Broudin Chloe , Ravel Patrice , De Guillebon Eléonore , Mauge Laetitia , Helley Dominique , Jabla Bernd , Chaput Nathalie , Albiges Laurence , Katsahian Sandrine , Adam Julien , Mejean Arnaud , Adotevi Olivier , Vano Yann A. , Oudard Stéphane , Tartour Eric
Published: September 2022

Purpose:

CD70 is a costimulatory molecule known to activate CD27-expressing T cells. CD27–CD70 interaction leads to the release of soluble CD27 (sCD27). Clear-cell renal cell carcinoma (ccRCC) expresses the highest levels of CD70 among all solid tumors; however, the clinical consequences of CD70 expression remain unclear.

Experimental Design:

Tumor tissue from 25 patients with ccRCC was assessed for the expression of CD27 and CD70 in situ using multiplex immunofluorescence. CD27+ T-cell phenotypes in tumors were analyzed by flow cytometry and their gene expression profile were analyzed by single-cell RNA sequencing then confirmed with public data. Baseline sCD27 was measured in 81 patients with renal cell carcinoma (RCC) treated with immunotherapy (35 for training cohort and 46 for validation cohort).

Results:

In the tumor microenvironment, CD27+ T cells interacted with CD70-expressing tumor cells. Compared with CD27− T cells, CD27+ T cells exhibited an apoptotic and dysfunctional signature. In patients with RCC, the intratumoral CD27–CD70 interaction was significantly correlated with the plasma sCD27 concentration. High sCD27 levels predicted poor overall survival in patients with RCC treated with anti–programmed cell death protein 1 in both the training and validation cohorts but not in patients treated with antiangiogenic therapy.

Conclusions:

In conclusion, we demonstrated that sCD27, a surrogate marker of T-cell dysfunction, is a predictive biomarker of resistance to immunotherapy in RCC. Given the frequent expression of CD70 and CD27 in solid tumors, our findings may be extended to other tumors.
An Ex Vivo Organotypic Culture Platform for Functional Interrogation of Human Appendiceal Cancer Reveals a Prominent and Heterogenous Immunological Landscape
Authors: Weitz Jonathan , Hurtado de Mendoza Tatiana , Tiriac Herve , Lee James , Sun Siming , Garg Bharti , Patel Jay , Li Kevin , Baumgartner Joel , Kelly Kaitlin J. , Veerapong Jula , Hosseini Mojgan , Chen Yuan , Lowy Andrew M.
Published: September 2022

Purpose:

Epithelial neoplasms of the appendix are difficult to study preclinically given their low incidence, frequent mucinous histology, and absence of a comparable organ in mice for disease modeling. Although surgery is an effective treatment for localized disease, metastatic disease has a poor prognosis as existing therapeutics borrowed from colorectal cancer have limited efficacy. Recent studies reveal that appendiceal cancer has a genomic landscape distinct from colorectal cancer and thus preclinical models to study this disease are a significant unmet need.

Experimental Design:

We adopted an ex vivo slice model that permits the study of cellular interactions within the tumor microenvironment. Mucinous carcinomatosis peritonei specimens obtained at surgical resection were cutoff using a vibratome to make 150-μm slices cultured in media.

Results:

Slice cultures were viable and maintained their cellular composition regarding the proportion of epithelial, immune cells, and fibroblasts over 7 days. Within donor specimens, we identified a prominent and diverse immune landscape and calcium imaging confirmed that immune cells were functional for 7 days. Given the diverse immune landscape, we treated slices with TAK981, an inhibitor of SUMOylation with known immunomodulatory functions, in early-phase clinical trials. In 5 of 6 donor samples, TAK981-treated slices cultures had reduced viability, and regulatory T cells (Treg). These data were consistent with TAK981 activity in purified Tregs using an in vitro murine model.

Conclusions:

This study demonstrates an approach to study appendiceal cancer therapeutics and pathobiology in a preclinical setting. These methods may be broadly applicable to the study of other malignancies.
Abstract IA26: Understanding CLL and Richter’s syndrome biology through mouse models of human genetics
Authors: ten Hacken Elisa
Published: September 2022

Large-scale human next generation sequencing studies have progressively defined the complex genetic landscape of chronic lymphocytic leukemia (CLL) and Richter’s syndrome (RS), yet the difficulties in rapid manipulation of primary CLL cells and the limited availability of cell lines have challenged studies aimed at interrogating the function of the so-called disease “drivers”, defined in human studies through statistical inference. Mouse models represent powerful tools to study mechanisms of normal and malignant B cell biology. Conditional knock-in/knock-out systems have allowed B-cell restricted modeling of CLL genetic lesions, with MDR mice being the first example of a genetically-faithful CLL model, recapitulating the most common genomic aberration of human disease, del(13q). Since then, a series of models generated by our group and others have allowed to uncover key functional changes underlying disease onset and transformation, including in the context of combined mutation in the splicing factor Sf3b1 and the DNA damage response gene Atm, sole mutation in the B cell developmental factor Ikzf3, the nuclear export receptor Xpo1, the ribosomal protein Rps15, and the DNA methyltransferase Dnmt3a (the latter not a CLL lesion per se, but recapitulating disordered methylation patterns typical of CLL). The CRISPR-Cas9 gene editing technology has further enabled B-cell restricted introduction of multiple combinatorial loss-of-function lesions, allowing the generation of faithful models not only of CLL but, importantly, of transformation into RS. In this presentation, I will describe the application of mouse models to studies of CLL and RS pathogenesis and treatment, with a focus on key aspects uncovered through the characterization of genetically- inspired mouse lines of recurrent disease drivers. I will provide a perspective on how these novel models combined with new technology allowed the dissection of mechanisms of disease evolution and response to therapy with greater depth than previously possible, representing valuable platforms for functional genomic analyses.

Citation Format: Elisa ten Hacken. Understanding CLL and Richter’s syndrome biology through mouse models of human genetics [abstract]. In: Proceedings of the Third AACR International Meeting: Advances in Malignant Lymphoma: Maximizing the Basic-Translational Interface for Clinical Application; 2022 Jun 23-26; Boston, MA. Philadelphia (PA): AACR; Blood Cancer Discov 2022;3(5_Suppl):Abstract nr IA26.
Abstract IA31: A multi-scale, multiomic atlas of human and follicular lymphoma lymph nodes
Authors: Radtke Andrea J.
Published: September 2022

Reference atlases, molecular and spatial maps of mammalian tissues, are critical resources for discovery efforts and translational research. Their utility is dependent on operationalizing the resulting data by identifying cell types, histological patterns, and predictive biomarkers underlying health and disease. The human lymph node (LN) offers a compelling use case because of its importance in immunity, structural and cellular diversity, and neoplastic involvement. One hematological malignancy, follicular lymphoma (FL), evolves from developmentally blocked germinal center B cells residing in and trafficking through these tissues. To promote survival and immune escape, tumor B cells undergo significant genetic changes and extensively remodel the lymphoid microenvironment. Here, we present an integrated portrait of healthy and FL LNs using multiple genomic and advanced imaging technologies. By leveraging the strengths of each platform, we identified several tumor-specific features and microenvironmental patterns enriched in individuals who experience early relapse, the most high-risk of FL patients.

Citation Format: Andrea J. Radtke. A multi-scale, multiomic atlas of human and follicular lymphoma lymph nodes [abstract]. In: Proceedings of the Third AACR International Meeting: Advances in Malignant Lymphoma: Maximizing the Basic-Translational Interface for Clinical Application; 2022 Jun 23-26; Boston, MA. Philadelphia (PA): AACR; Blood Cancer Discov 2022;3(5_Suppl):Abstract nr IA31.
Human Papillomavirus Impact on Temporal Treatment Trends in Oropharyngeal Carcinoma: 2010–2016
Authors: Amin Neha , Thompson Joshua , Goloubeva Olga , Witek Matthew , Taylor Rodney J. , Wolf Jeffrey S. , Moyer Kelly , Mehra Ranee , Hatten Kyle M.
Published: September 2022

Introduction: The study objective was to identify practice patterns in oropharyngeal cancer management from 2010 to 2016 among human papillomavirus (HPV)-associated and non-HPV-associated oropharyngeal squamous-cell carcinoma (OPSCC) patients. Methods: The National Cancer Database was utilized to identify OPSCC patients from 2010 to 2016. Frequency distributions and multivariable analyses were generated to identify practice patterns and predictors of treatment modality. Results: A total of 35,956 patients with nonmetastatic OPSCC were included. HPV status was not associated with a treatment modality preference. At academic centers, the proportion of HPV-associated OPSCC patients versus non-HPV-associated OPSCC patients undergoing surgical management was similar (35.7%; 35.9%). Community cancer programs treated patients less often surgically but with no significant treatment preference based on HPV status. Within each facility type, HPV status was not a predictor of surgical or nonsurgical management. Conclusion: HPV association does not appear to significantly influence treatment modality preference among OPSCC patients. The proportion of OPSCC patients undergoing surgical treatment declined from 2010 to 2016.
A Pilot Study Examining the Effects of Ischemic Conditioning on Walking Capacity and Lower Extremity Muscle Performance in Patients with Claudication
Authors: Loo Rory J. , Wohlauer Max V. , Tarima Sergey S. , Weseman Elizabeth , Nguyen Jennifer N. , Mansukhani Neel A. , Durand Matthew J.
Published: September 2022

Introduction: This study investigated whether a novel therapy called ischemic conditioning (IC) improves walking capacity and lower extremity muscle performance in patients with peripheral vascular disease who experience intermittent claudication. Methods: Forty-three patients with claudication were enrolled and received either IC or IC Sham for 2 weeks in this randomized, controlled, double-blinded, prospective study. IC sessions involved five cycles of alternating 5-min inflations of a blood pressure cuff to 225 mm Hg (25 mm Hg for IC Sham) and 5-min deflations, around the thigh of the affected lower extremity. Results: There was no difference in the change in claudication onset time (Δ = 114 ± 212 s IC vs. 104 ± 173 s IC Sham; p = 0.67) or peak walking time (Δ = 42 ± 139 s IC vs. 12 ± 148 s IC Sham; p = 0.35) between the IC and IC Sham groups. At the level of the knee, participants in the IC group performed more work (Δ = 3,029 ± 4,999 J IC vs. 345 ± 2,863 J IC Sham; p = 0.03) and displayed a greater time to muscle fatigue (Δ = 147 ± 221 s IC vs. −27 ± 236 s IC Sham; p = 0.01). Discussion/Conclusion: In patients with claudication, IC improved total work performed and time to fatigue at the knee but did not change walking parameters.
Postcolonoscopy Colorectal Cancer in a Referral Center for Colorectal Cancer: Prevalence and Risk Factors
Authors: Gonçalves Margarida Gomes , Silva Joana , Carvalho Tânia , Costa Dalila , Gonçalves Raquel , Rebelo Ana
Published: September 2022

Background and Aims: Colonoscopy is effective to detect and remove colorectal lesions. However, after a negative colonoscopy, cancers could be detected during the interval follow-up. This study was designed to identify characteristics and risk factors for postcolonoscopy colorectal cancer – interval type. Methods: Medical records of individuals who were newly diagnosed with colorectal cancer between January 2018 and December 2019 were reviewed. Clinical, demographic, and endoscopic variables were analyzed. Those with the diagnosis of colorectal cancer between two consecutive colonoscopies performed within the appropriated surveillance range were considered to have postcolonoscopy colorectal cancer – interval type. A comparison between the group of patients with non-postcolonoscopy colorectal cancer – interval type and the group of patients with postcolonoscopy colorectal cancer – interval type was then performed. Results: During the study period, 491 patients were newly diagnosed with colorectal cancer. Among them, 61 (12.4%) had postcolonoscopy colorectal cancer – interval subtype. Postcolonoscopy colorectal cancer – interval type was three times more prevalent on the proximal colon (p = 0.014) and was associated with the presence of two or more cardiovascular risk factors (aOR = 4.25; p = 0.016), cholecystectomy in the past (aOR = 10.09; p = 0.019), and family history of colorectal cancer on a first-degree relative (aOR = 4.25; p = 0.006). Moreover, isolated cardiovascular risk factors revealed a protective effect for the absence of all cardiovascular risk factors (aOR = 20; p = 0.034). The ROC curve associated with the multivariate model revealed a predictive power of 77.8% (p < 0.001). Conclusions: Postcolonoscopy colorectal cancer – interval type is more common in the proximal colon and in patients with a family history (first-degree relative) of colorectal cancer, two or more cardiovascular risk factors, and a history of cholecystectomy. All of these are easily detectable in clinical practice and may be of extreme importance in the control of postcolonoscopy colorectal cancer in the near future.