bluuebunny/crossref_metadata_2025 huggingface dataset:
Untargeted Metabolomics Reveals Alterations of Rhythmic Pulmonary Metabolism in IPF
Authors: Sun Wei , Ren Jiuqiang , Jia Zixian , Liang Puyang , Li Shengxi , Song Meiyue , Cao Yinghao , Chen Haoran , Luo Qiang , Yang Lifeng , Wang Jing , Wang Chen , Wang Lin
Published: October 2023

Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive condition characterized by the impairment of alveolar epithelial cells. Despite continued research efforts, the effective therapeutic medication is still absent due to an incomplete understanding of the underlying etiology. It has been shown that rhythmic alterations are of significant importance in the pathophysiology of IPF. However, a comprehensive understanding of how metabolite level changes with circadian rhythms in individuals with IPF is lacking. Here, we constructed an extensive metabolite database by utilizing an unbiased reference system culturing with 13C or 15N labeled nutrients. Using LC-MS analysis via ESI and APCI ion sources, 1300 potential water-soluble metabolites were characterized and applied to evaluate the metabolic changes with rhythm in the lung from both wild-type mice and mice with IPF. The metabolites, such as glycerophospholipids and amino acids, in WT mice exhibited notable rhythmic oscillations. The concentrations of phospholipids reached the highest during the fast state, while those of amino acids reached their peak during fed state. Similar diurnal variations in the metabolite rhythm of amino acids and phospholipids were also observed in IPF mice. Although the rhythmic oscillation of metabolites in the urea cycle remained unchanged, there was a significant up-regulation in their levels in the lungs of IPF mice. 15N-ammonia in vivo isotope tracing further showed an increase in urea cycle activity in the lungs of mice with IPF, which may compensate for the reduced efficiency of the hepatic urea cycle. In sum, our metabolomics database and method provide evidence of the periodic changes in lung metabolites, thereby offering valuable insights to advance our understanding of metabolic reprogramming in the context of IPF.
Process Optimization for Robotic Ultrasonic Strengthening of Aviation Blade Surfaces Based on Intelligent Compliance Control
Authors: Fang Shanxiang , Zhu Yukai , Zhang Qinjian , Zhang Yong
Published: October 2023

In order to enhance the automation level and achieve high precision in the ultrasonic strengthening of aviation blade surfaces, this study focuses on investigating the intelligent control strategy and optimizing the machining parameters for robotic ultrasonic surface strengthening. By designing an intelligent compliance control method, the end-effector can achieve the compliant output of contact force. The fuzzy PID control method is used to optimize the regulation performance of the compliant force control system. This compliance control strategy enables the optimization of the compliance device, effectively improving the static and dynamic characteristics of the compliance controller. Based on this, an experimental method (RSM) is designed to analyze the interaction effects of contact force, feed rate, and repetition times on the surface quality of the blade. The optimal combination of robotic strengthening parameters is determined, providing a practical reference for the application of robotic compliance control in the ultrasonic strengthening of aviation blade surfaces.
Phylogenetic Characterization of Rabies Virus Field Isolates Collected from Animals in European Russian Regions in 2009–2022
Authors: Chupin Sergei A. , Sprygin Alexandr V. , Zinyakov Nikolay G. , Guseva Nelly A. , Shcherbinin Sergey V. , Korennoy Fedor I. , Adelshin Renat V. , Mazloum Ali , Sukharkov Andrey Y. , Nevzorova Victoria V.
Published: October 2023

Rabies is a fatal disease of mammals that poses a high zoonotic risk to humans as well. The distribution of rabies is mainly driven by host animal migration and human-mediated dispersion. To contribute to the global understanding of the rabies virus (RABV) molecular epidemiology, 94 RABV field isolates collected from animals in 13 European Russian regions were phylogenetically characterized using the nearly full-size N gene nucleotide sequences. According to phylogenetic inferences, all isolates belonged to one of the two established phylogenetic groups, either group C (n = 54) or group D (n = 40), which are part of the clade Cosmopolitan of RABVs. Some representatives of group C collected from regions located far apart from each other had a remarkably high level of nucleotide identity. The possibility of the contribution of local bat species to the distribution of RABVs was discussed. Interestingly, over the years, the fraction of group D isolates has been constantly decreasing compared with that of group C isolates. The phylogenetic insights generated herein might have an important contribution to the control and surveillance of animal rabies epidemiology in the region.
Gut Microbiota Associated with Clostridioides difficile Carriage in Three Clinical Groups (Inflammatory Bowel Disease, C. difficile Infection and Healthcare Workers) in Hospital Field
Authors: Martinez Elisa , Crevecoeur Sebastien , Thirion Carine , Grandjean Jessica , Fall Papa Abdoulaye , Hayette Marie-Pierre , Michel Moutschen , Taminiau Bernard , Louis Edouard , Daube Georges
Published: October 2023

Clostridioides difficile is an anaerobic spore-forming Gram-positive bacterium. C. difficile carriage and 16S rDNA profiling were studied in three clinical groups at three different sampling times: inflammatory bowel disease (IBD) patients, C. difficile infection (CDI) patients and healthcare workers (HCWs). Diversity analysis was realized in the three clinical groups, the positive and negative C. difficile carriage groups and the three analysis periods. Concerning the three clinical groups, β-diversity tests showed significant differences between them, especially between the HCW group and IBD group and between IBD patients and CDI patients. The Simpson index (evenness) showed a significant difference between two clinical groups (HCWs and IBD). Several genera were significantly different in the IBD patient group (Sutterella, Agathobacter) and in the CDI patient group (Enterococcus, Clostridioides). Concerning the positive and negative C. difficile carriage groups, β-diversity tests showed significant differences. Shannon, Simpson and InvSimpson indexes showed significant differences between the two groups. Several genera had significantly different relative prevalences in the negative group (Agathobacter, Sutterella, Anaerostipes, Oscillospira) and the positive group (Enterococcus, Enterobacteriaceae_ge and Enterobacterales_ge). A microbiota footprint was detected in C. difficile-positive carriers. More experiments are needed to test this microbiota footprint to see its impact on C. difficile infection.
Unlocking the Viral Universe: Metagenomic Analysis of Bat Samples Using Next-Generation Sequencing
Authors: Roev German V. , Borisova Nadezhda I. , Chistyakova Nadezhda V. , Agletdinov Matvey R. , Akimkin Vasily G. , Khafizov Kamil
Published: October 2023

Next-generation sequencing technologies have revolutionized the field of virology by enabling the reading of complete viral genomes, extensive metagenomic studies, and the identification of novel viral pathogens. Although metagenomic sequencing has the advantage of not requiring specific probes or primers, it faces significant challenges in analyzing data and identifying novel viruses. Traditional bioinformatics tools for sequence identification mainly depend on homology-based strategies, which may not allow the detection of a virus significantly different from known variants due to the extensive genetic diversity and rapid evolution of viruses. In this work, we performed metagenomic analysis of bat feces from different Russian cities and identified a wide range of viral pathogens. We then selected sequences with minimal homology to a known picornavirus and used “Switching Mechanism at the 5′ end of RNA Template” technology to obtain a longer genome fragment, allowing for more reliable identification. This study emphasizes the importance of integrating advanced computational methods with experimental strategies for identifying unknown viruses to better understand the viral universe.
Application of Electromagnetic Methods for Reservoir Monitoring with Emphasis on Carbon Capture, Utilization, and Storage
Authors: Barajas-Olalde César , Adams Donald C. , Curcio Ana , Davydycheva Sofia , Klapperich Ryan J. , Martinez Yardenia , Paembonan Andri Y. , Peck Wesley D. , Strack Kurt , Soupios Pantelis
Published: October 2023

The Controlled-Source ElectroMagnetic (CSEM) method provides crucial information about reservoir fluids and their spatial distribution. Carbon dioxide (CO2) storage, enhanced oil recovery (EOR), geothermal exploration, and lithium exploration are ideal applications for the CSEM method. The versatility of CSEM permits its customization to specific reservoir objectives by selecting the appropriate components of a multi-component system. To effectively tailor the CSEM approach, it is essential to determine whether the primary target reservoir is resistive or conductive. This task is relatively straightforward in CO2 monitoring, where the injected fluid is resistive. However, for scenarios involving brine-saturated (water-wet) or oil-wet (carbon capture, utilization, and storage—CCUS) reservoirs, consideration must also be given to conductive reservoir components. The optimization of data acquisition before the survey involves analyzing target parameters and the sensitivity of multi-component CSEM. This optimization process typically includes on-site noise measurements and 3D anisotropic modeling. Based on our experience, subsequent surveys tend to proceed smoothly, yielding robust measurements that align with scientific objectives. Other critical aspects to be considered are using magnetotelluric (MT) measurements to define the overall background resistivities and integrating real-time quality assurance during data acquisition with 3D modeling. This integration allows the fine tuning of acquisition parameters such as acquisition time and necessary repeats. As a result, data can be examined in real-time to assess subsurface information content while the acquisition is ongoing. Consequently, high-quality data sets are usually obtained for subsequent processing and initial interpretation with minimal user intervention. The implementation of sensitivity analysis during the inversion process plays a pivotal role in ensuring that the acquired data accurately respond to the target reservoirs’ expected depth range. To elucidate these concepts, we present an illustrative example from a CO2 storage site in North Dakota, USA, wherein the long-offset transient electromagnetic method (LOTEM), a variation of the CSEM method, and the MT method were utilized. This example showcases how surface measurements attain appropriately upscaled log-scale sensitivity. Furthermore, the sensitivity of the CSEM and MT methods was examined in other case histories, where the target reservoirs exhibited conductive properties, such as those encountered in enhanced oil recovery (EOR), geothermal, and lithium exploration applications. The same equipment specifications were utilized for CSEM and MT surveys across all case studies.
From Dust to Disease: A Review of Respirable Coal Mine Dust Lung Deposition and Advances in CFD Modeling
Authors: Madureira Eurico , Aboelezz Ahmed , Su Wei-Chung , Roghanchi Pedram
Published: October 2023

The United States has witnessed a concerning surge in the incidence of diseases like Coal Workers’ Pneumoconiosis (CWP), despite numerous efforts aimed at prevention. This study delves into the realm of respiratory health by investigating the deposition of dust particles within the respiratory tract and lungs. By analyzing particles of varying sizes, shapes, velocities, and aerodynamic diameters, we aim to gain a comprehensive understanding of their impact on deposition patterns. This insight could potentially drive changes in dust exposure protocols within mining environments and improve monitoring practices. The interplay of several critical factors, including particle characteristics and an individual’s breathing patterns, plays a pivotal role in determining whether particles settle in the lungs or are exhaled. This paper provides a comprehensive literature review on Respirable Coal Mine Dust (RCMD), with a specific focus on examining particle deposition across different regions of the airway system and lungs. Additionally, we explore the utility of Computational Fluid Dynamics (CFD) in simulating particle behavior within the respiratory system. Predicting the precise behavior of dust particles within the respiratory airway poses a significant challenge. However, through numerical simulations, we aspire to enhance our understanding of strategies to mitigate total lung deposition by comprehensively modeling particle interactions within the respiratory system.
Nano-Additive Manufacturing and Non-Destructive Testing of Nanocomposites
Authors: She Yulong , Tang Jie , Wang Chaoyang , Wang Zhicheng , Huang Zhengren , Yang Yong
Published: October 2023

In the present work, the recent advancements in additive manufacturing (AM) techniques for fabricating nanocomposite parts with complex shaped structures are explained, along with defect non-destructive testing (NDT) methods. A brief overview of the AM processes for nanocomposites is presented, grouped by the type of feedstock used in each technology. This work also reviews the defects in nanocomposites that can affect the quality of the final product. Additionally, a detailed description of X-CT, ultrasonic phased array technology, and infrared thermography is provided, highlighting their potential application in non-destructive inspection of nanocomposites in the future. Lastly, it concludes by offering recommendations for the development of NDT methods specifically tailored for nanocomposites, emphasizing the need to utilize NDT methods for optimizing nano-additive manufacturing process parameters, developing new NDT techniques, and enhancing the resolution of existing NDT methods.
Fruit Pouch Consumption Does Not Associate with Early Manifestations of Allergic Disease
Authors: Fredriksson Emmy , Bodén Stina , Domellöf Magnus , West Christina E.
Published: October 2023

Consumption of acidic fruit pouches in infancy may damage the epithelial barrier in the gastrointestinal tract and is suggested to increase allergy risk. We aimed to explore if a high fruit pouch consumption is associated with a higher incidence of early allergic manifestations. We included 2959 parent–child dyads from the Swedish prospective, population-based NorthPop birth cohort study with parentally reported data on frequency of fruit pouch consumption at 9 months of age, as well as parentally reported eczema, wheeze, physician-diagnosed asthma, and food allergy in the first 18 months of life. Immunoglobulin E levels (IgE) in serum (n = 1792), as response to a food mix and an inhalant mix, were determined at age 18 months. Compared with no consumption, daily consumption of one or more pouches at 9 months of age was associated with inhalant sensitization (odds ratio (OR) 2.27, 95% confidence interval (CI) 1.06–4.87, n = 1792) but did not remain significant in the multivariable adjusted model (aOR 2.08, 95% CI 0.95–4.53, n = 1679). There were no associations between fruit pouch consumption and allergic manifestations at this young age. This study suggests that fruit pouch consumption is not associated with allergic phenotypes or IgE sensitization in early childhood.
Special Issue “Novel Anti-Proliferative Agents”
Authors: Onnis Valentina
Published: October 2023

Cancer is a disease that can affect any organ and spread to other nearby or distant organs [...]
Salinity Stress Tolerance in Plants
Authors: Trejo-Téllez Libia Iris
Published: October 2023

Soil salinization negatively impacts plant development and induces land degradation, thus affecting biodiversity, water quality, crop production, farmers’ well-being, and the economic situation in the affected region. Plant germination, growth, and productivity are vital processes impaired by salinity stress; thus, it is considered a serious threat to agriculture. The extent to which a plant is affected by salinity depends mainly on the species, but other factors, including soil attributes, water, and climatic conditions, also affect a plant’s ability to tolerate salinity stress. Unfortunately, this phenomenon is expected to be exacerbated further by climate change. Consequently, studies on salt stress tolerance in plants represent an important theme for the present Special Issue of Plants. The present Special Issue contains 14 original contributions that have documented novel discoveries regarding induced or natural variations in plant genotypes to cope with salt stress, including molecular biology, biochemistry, physiology, genetics, cell biology, modern omics, and bioinformatic approaches. This Special Issue also includes the impact of biostimulants on the biochemical, physiological, and molecular mechanisms of plants to deal with salt stress and on the effects of salinity on plant nutrient status. We expect that readers and academia will benefit from all the articles included in this Special Issue.
Tropical Red Macroalgae Cultivation with a Focus on Compositional Analysis
Authors: Augyte Simona , Sims Neil A. , Martin Keelee , Van Wychen Stefanie , Panczak Bonnie , Alt Hannah , Nelson Robert , Laurens Lieve M. L.
Published: October 2023

To create carbon efficient sources of bioenergy feedstocks and feedstuff for aquaculture and terrestrial livestock, it is critical to develop and commercialize the most efficient seaweed cultivation approach with a sustainable nutrient input supply. Here, we present data for a novel, onshore tropical macroalgae cultivation system, based on influent deep seawater as the nutrient and carbon sources. Two red algal species were selected, Agardhiella subulata and Halymenia hawaiiana, as the basis for growth optimization. Highest productivity in small-scale cultivation was demonstrated with A. subulata in the 10% deep seawater (64.7 µg N L−1) treatment, growing at up to 26% specific growth rate day−1 with highest yields observed at 247.5 g m−2 day−1 fresh weight. The highest yields for H. hawaiiana were measured with the addition of 10% deep seawater up to 8.8% specific growth rate day−1 and yields at 63.3 g fresh weight m−2 day−1 equivalent. Biomass should be culled weekly or biweekly to avoid density limitations, which likely contributed to a decrease in SGR over time. With a measured 30–40% carbon content of the ash-free dry weight (20–30% of the dry weight) biomass, this translates to an almost 1:1 CO2 capture to biomass ratio. The compositional fingerprint of the high carbohydrate content of both Agardhiella and Halymenia makes for an attractive feedstock for downstream biorefinery applications. By focusing on scaling and optimizing seaweed farming technologies for large-scale onshore farms, the opportunities for yield potential, adaptability to cultivation conditions, and meeting global sustainability goals through novel, carbon-negative biomass sources such as seaweed can be realized.
Receptor-Targeted Carbon Nanodot Delivery through Polymer Caging and Click Chemistry-Supported LRP1 Ligand Attachment
Authors: Zhang Fengrong , Benli-Hoppe Teoman , Guo Wei , Seidl Johanna , Wang Yi , Huang Rongqin , Wagner Ernst
Published: October 2023

Carbon nanodots present resistance to photobleaching, bright photoluminescence, and superior biocompatibility, making them highly promising for bioimaging applications. Herein, nanoprobes were caged with four-armed oligomers and subsequently modified with a novel DBCO–PEG-modified retro-enantio peptide ligand reL57, enhancing cellular uptake into U87MG glioma cells highly expressing low-density lipoprotein receptor-related protein 1 (LRP1). A key point in the development of the oligomers was the incorporation of ε-amino-linked lysines instead of standard α-amino-linked lysines, which considerably extended the contour length per monomer. The four-armed oligomer 1696 was identified as the best performer, spanning a contour length of ~8.42 nm for each arm, and was based on an altering motive of two cationic ε-amidated lysine tripeptides and two tyrosine tripeptides for electrostatic and aromatic stabilization of the resulting formulations, cysteines for disulfide-based caging, and N-terminal azidolysines for click-modification. This work highlights that well-designed four-armed oligomers can be used for noncovalent coating and covalent caging of nanoprobes, and click modification using a novel LRP1-directed peptide ligand facilitates delivery into receptor-expressing target cells.
Structure and Mechanical Response of Polybutylcarbosilane Dendrimers Confined in a Flat Slit: Effect of Molecular Architecture and Generation Number
Authors: Kurbatov Andrey O. , Balabaev Nikolay K. , Litvin Kirill A. , Kramarenko Elena Yu.
Published: October 2023

Due to the absence of specific interactions, carbosilane dendrimers are ideal models to study the effect of a hyperbranched regular structure on the molecular response to external influences. In this work, we have studied the conformational behavior of single polybutylcarbosilane dendrimers under confinement between impermeable flat surfaces using atomistic molecular dynamics simulations. Dendrimers of different generations belonging to two homologous series with a tetra-functional core and three- and four-functional branches were simulated. The analysis of the dependence of the internal energy of the dendrimers on the wall distance allowed us to determine the critical degree of compression at which the dendrimers are able to change their shape without energy loss. The effects of generation number and branching functionality on the number of wall contacts, density distribution and shape changes were elucidated. It was found that for high generation dendrimers, the inner layers are not accessible for external interaction. It was shown that the excess stresses occurring at high compressions are concentrated in the structural center of the dendrimer. The nature of the elastic response, which is strongly nonlinear, was analyzed at different compressions depending on the dendrimer architecture and generation. We believe that our results are useful for further studies of dendrimer films under compression and can also serve as a basis for developing model concepts to describe the dynamics of dendrimer melts.
Fluoride Release and Rechargeability of Poly(lactic acid) Composites with Glass Ionomer Cement
Authors: Wongphattarakul Sudarat , Kuson Rungroj , Sastraruji Thanapat , Suttiat Kullapop
Published: October 2023

This study investigates the fluoride release, rechargeability and degradation behaviors of newly developed anticariogenic poly(lactic acid) (PLA) composites. The PLA composite with various concentrations (0%, 5%, 10%, 15% and 20% by weight) of glass ionomer cement (GIC) and sodium fluoride (NaF) were prepared using solvent casting method. The fluoride release, fluoride rechargeability and degradation behavior were evaluated. All experimental groups demonstrated fluoride-releasing ability. The highest level of fluoride ions released was found in PLA composite with sodium fluoride (PLA/NaF). Following the 28-day period, both groups showed a gradual reduction in fluoride ion released, ranging between 0.03 ± 0.01 and 0.53 ± 0.06 ppm, although remaining within the effective range for tooth remineralization. However, the rechargeability was only observed in PLA composite with GIC (PLA/GIC). Following an eight-week in vitro degradation test, all PLA/NaF groups displayed a significantly higher percentage of weight change and water absorption compared to the PLA/GIC and the control group. In SEM analysis, the formation of surface porosities was clearly noticed in all PLA/NaF. All specimens retained their structural integrity throughout the study. In conclusion, the newly developed PLA/GIC displays promising possibilities as an anticariogenic material. Furthermore, the rechargeability of these ions are repeatable, ensuring their long-term utility.
Simulating the Rapid Devolatilization of Mineral-Free Lignins
Authors: Niksa Stephen
Published: October 2023

Lig-FC is a network depolymerization mechanism for the rapid primary devolatilization of mineral-free lignins that has already been validated with test data on 16 lignin samples. This paper expands the validation with an additional 13 lignins, including cases that applied different lignin preparations to the same feedstock. The validations reported here cover 27 mineral-free lignins for temperatures to 1150 °C, heating rates from 30 to 8000 °C/s, contact times after heatup to 90 s, and pressures from vacuum to 0.13 MPa. Lig-FC accurately depicts the impacts of lignin quality, heating rate, temperature, contact time, and pressure on the major products and oils’ molecular weight distributions (MWDs). All raw lignins contain abundant oil precursors that are released as oils via flash distillation as soon as a flow of noncondensables carries them into the free stream. Consequently, lignin MWD is an essential aspect of lignin constitution because it determines the inventory of inherent volatile chains subject to unhindered flash distillation. Lighter lignin MWDs have larger inherent inventories and therefore produce more oils than heavier MWDs at the onset of devolatilization. Oil yields diminish and char yields increase for progressively heavier MWDs and heavier mean monomer weights and for lignins with relatively less H and more O compared to C.
Evaluating the Cooling Efficiency of Polymer Injection Molds by Computer Simulation Using Conformal Channels
Authors: Vargas-Isaza Carlos , Benitez-Lozano Adrian , Rodriguez Johnnatan
Published: October 2023

Injection molds are production tools that require detailed analysis based on the quality of the resulting part, the impact on cycle times, and the expected production volume. Cooling channels also play a critical role in mold performance and product quality as they largely determine cycle time. Designs that incorporate conformal cooling channel (CCC) geometries that conform to or align with the part contour are currently being explored as an alternative to conventional cooling channel designs in injection molds. In this study, a simulation of CCC geometries was performed and their effects on mold temperatures and warpage were investigated. Two cross-sectional geometries, circular and square, were selected for a three-factor level design of experiments (DOE) analysis. The response variables used were mold temperatures and part warpage. A cup-shaped part with upper and lower diameters of 54 and 48 mm, respectively, a height of 23 mm and a thickness of 3 mm was used for the injection molded part. A comparison was also made between two materials for the injection mold, steel and polycarbonate. The DOE results showed that the distance between the CCC and the injected part and the diameter or side of the square have significant effects on the response variables for both systems (steel and polycarbonate molds). In addition, a comparison between conventional and conformal cooling channels was analyzed using a cup-shaped part and a less rigid part geometry. The finite element simulation results show a 9.26% reduction in final warpage in the cup-shaped part using CCCs compared with the conventional cooling methods in steel. When using parts with lower geometry stiffness, the use of CCCs reduced final part warpage by 32.4% in metal molds and by 59.8% in polymer molds.
Automated Production at Scale of Induced Pluripotent Stem Cell-Derived Mesenchymal Stromal Cells, Chondrocytes and Extracellular Vehicles: Towards Real-Time Release
Authors: Herbst Laura , Groten Ferdinand , Murphy Mary , Shaw Georgina , Nießing Bastian , Schmitt Robert H.
Published: October 2023

Induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) are amenable for use in a clinical setting for treatment of osteoarthritis (OA), which remains one of the major illnesses worldwide. Aside from iPSC-derived iMSCs, chondrocytes (iCHO) and extracellular vesicles (EV) are also promising candidates for treatment of OA. Manufacturing and quality control of iPSC-derived therapies is mainly manual and thus highly time consuming and susceptible to human error. A major challenge in translating iPSC-based treatments more widely is the lack of sufficiently scaled production technologies from seeding to fill-and-finish. Formerly, the Autostem platform was developed for the expansion of tissue-derived MSCs at scale in stirred tank bioreactors and subsequent fill-and-finish. Additionally, the StemCellDiscovery platform was developed to handle plate-based cultivation of adherent cells including their microscopic analysis. By combining the existing automation technology of both platforms, all required procedures can be integrated in the AutoCRAT system, designed to handle iPSC expansion, differentiation to iMSCs and iCHOs, pilot scale expansion, and formulation of iMSCs as well as extracellular vesicles and their purification. Furthermore, the platform is equipped with several in-line and at-line assays to determine product quality, purity, and safety. This paper highlights the need for adaptable and modular automation concepts. It also stresses the importance of ensuring safety of generated therapies by incorporating automated release testing and cleaning solutions in automated systems. The adapted platform concepts presented here will help translate these technologies for clinical production at the necessary scale.
Caught in Narrative Patterns? Analysis of the Swiss News Coverage of Christians, Muslims, and Jews
Authors: Koch Carmen , Hüsser Angelica
Published: October 2023

The media coverage of religious communities can have an impact on society and contribute to the manifestation of certain images in society. Since recent surveys show social tensions concerning Muslims and Jews, it is important to monitor media coverage. In this study, we investigate the images of Christianity, Islam, and Judaism in the news media. Theoretically, the approaches of framing and narrative patterns are used. The study was conducted in two steps. (1) Focus group discussions were held with members of the three religions, which showed, among other things, that all three religious groups do not feel adequately represented. (2) A quantitative content analysis of selected Swiss newspaper articles (online and print) was conducted. The results of the content analysis suggest that a different perspective is taken depending on the religious community: while Christianity is framed from an internal perspective, Judaism and Islam are framed from an external perspective.
Inversion of the Lunar Subsurface Rock Abundance Using CE-2 Microwave Brightness Temperature Data
Authors: Yang Wei , Hu Guoping , Yang Fan , Zheng Wenchao
Published: October 2023

The rock strongly affects the surface and subsurface temperature due to its different thermophysical properties compared to the lunar regolith. The brightness temperature (TB) data observed by Chang’E-1 (CE-1) and Chang’E-2 (CE-2) microwave radiometers (MRM) give us a chance to retrieve the lunar subsurface rock abundance (RA). In this paper, a thermal conductivity model with an undetermined parameter β of the mixture has been employed to estimate the physical temperature profile of the mixed layer (rock and regolith). Parameter β and the physical temperature profile of the mixed layer are constrained by the Diviner Channel 7 observations. Then, the subsurface RA on the 16 large (Diameter > 20 km) Copernican-age craters of the Moon is extracted from the average nighttime TB of the CE-2 37 GHz channel based on our previous rocky TB model. Two conclusions can be derived from the results: (1) the subsurface RA values are usually greater than the surface RA values retrieved from Diviner observations of the studied craters; (2) the spatial distribution of subsurface RA extracted from CE-2 MRM data is not necessarily consistent with the surface RA detected by Diviner data. For example, there are similar RA spatial distributions on both the surface and subsurface in Giordano Bruno, Necho, and Aristarchus craters. However, the distribution of subsurface RA is obviously different from that of surface RA for Copernicus, Ohm, Sharonov, and Tycho craters.
An Automated System to Monitor River Ice Conditions Using Visible Infrared Imaging Radiometer Suite Imagery
Authors: Temimi Marouane , Abdelkader Mohamed , Tounsi Achraf , Chaouch Naira , Carter Shawn , Sjoberg Bill , Macneil Alison , Bingham-Maas Norman
Published: October 2023

This study presents an innovative, automated deep learning-based technique for near real-time satellite monitoring of river ice conditions in northern watersheds of the United States and Canada. The method leverages high-resolution imagery from the VIIRS bands onboard the NOAA-20 and NPP satellites and employs the U-Net deep learning algorithm for the semantic segmentation of images under varying cloud and land surface conditions. The system autonomously generates detailed maps delineating classes such as water, land, vegetation, snow, river ice, cloud, and cloud shadow. The verification of system outputs was performed quantitatively by comparing with existing ice extent maps in the northeastern US and New Brunswick, Canada, yielding a Probability of Detection of 0.77 and a False Alarm rate of 0.12, suggesting commendable accuracy. Qualitative assessments were also conducted, corroborating the reliability of the system and underscoring its utility in monitoring hydraulic and hydrological processes across northern watersheds. The system’s proficiency in accurately capturing the phenology of river ice, particularly during onset and breakup times, testifies to its potential as a valuable tool in the realm of river ice monitoring.
Colocation in Time and Space of High-Precision Two-Way Optical and Microwave Observations for Calibration of a Microwave Ranging Link—The ACES Mission Case
Authors: Vollmair Peter , Schlicht Anja , Hugentobler Urs
Published: October 2023

The ACES mission of the European Space Agency combines optical and microwave-based geodetic observation techniques with highly accurate atomic clocks to achieve a new level of accuracy for geodesy and fundamental physics applications. In addition, the combination of two high-precision measurement techniques provides an even more exciting insight into their errors. Fundamental physics is particularly interested in experiments that require high precision between the results of the successive passes of a satellite. An example of such an experiment is the determination of gravitational redshift. Geodesy applications, in contrast, require both high accuracy and precision. Especially for applications like precise ranging or time synchronization, all possible error influences must be characterized and determined with high precision. Therefore, electronic delays of microwave link terminals pose a challenge to achieving high accuracy. They must, therefore, be calibrated, and the stability of the electronic delays must be monitored. While optical observation techniques can be calibrated sufficiently on the ground, the calibration of microwave measurements before a launch is not precise enough, and continuous monitoring is also not possible. In this study, four calibration methods were tested, all based on colocating optical and microwave measurements onboard a satellite and on the ground. The results of two methods achieved the required accuracy of 100 ps for time synchronization, with a mean error and standard deviation of better than 4 ps and 55 picoseconds, respectively. Correlations between the measured parameters were identified, and the impact of the different approaches on accuracy was investigated. It will be shown that the satellite-based colocation of two different geodetic observation techniques has clear advantages, and the calibration results achieved the required accuracy for geodetic applications in this simulation study.
Multi-Scale Influence Analysis of Urban Shadow and Spatial Form Features on Urban Thermal Environment
Authors: Lin Liqun , Deng Yangyan , Peng Man , Zhen Longxiang , Qin Shuwei
Published: October 2023

In urban thermal environment research (UTE), urban shadows formed by buildings and trees contribute to significant variations in thermal conditions, particularly during the mid-day period. This study investigated the multi-scale effects of indicators, including urban shadows, on UTE, focusing specifically on the mid-day hours. It integrated field temperature measurements and drone aerial data from multiple city blocks. Considering both urban shadows and direct solar radiation, a linear mixed-effects model was employed to study the multi-scale effects of urban morphological indicators. Results showed that: (1) UTE is a multi-scale, multi-factor phenomenon, with thermal effects manifesting at specific scales. Under shadow conditions, smaller scales (10–20 m) of landscape heterogeneity and larger scales (300–400 m) of landscape consistency better explained temperature variations mid-day. Conversely, under direct sunlight, temperature was primarily influenced by larger scales (150–300 m). (2) Trees significantly reduced temperature; 100% tree canopy cover within a 10-m radius reduced air temperatures by approximately 2 °C mid-day. However, there is no significant correlation between temperature and green spaces. (3) Building area and height were significantly correlated with temperature. Specifically, an increase in building area beyond 150 m, especially within a 300-m radius, leads to higher temperatures. Conversely, building height within a 10–20 m range exhibits significant cooling effects. These findings provide crucial reference data for micro-scale UTE investigations during mid-day hours and offer new strategies for urban planning and design.
Multilateralism under Fire: How Public Narratives of Multilateralism and Ideals of a Border-Free World Repudiate the Populist Re-Bordering Narrative
Authors: Mahendran Kesi , English Anthony , Nieland Sue
Published: October 2023

How do global multilateral arrangements such as the United Nations (UN) and World Health Organization (WHO), vital to post-pandemic recovery, connect to the public understanding of multilateralism? The Citizen Worldview Mapping Project (CWMP) conducted in England, Scotland and Sweden examines how the degree of migration–mobility interacts with worldviews. CWMP asked participants (N = 24) to rule the world using an online interactive world mapping tool. Citizens were first interviewed on their migration–mobility, then invited to draw or remove borders on the world to manage human mobility. Citizens then engaged in a dialogue with António Guterres’ 2018 address to the United Nations General Assembly on multilateralism. Dialogical analysis showed how, when empowered to rule the world, the majority of participants, irrespective of the degree of migration–mobility, expressed an ideal of a border-free world, even if they then went on to construct borders around the world. We understand this as a democratic dialogical ideal of a border-free world. Participants articulated rich narratives and social representations of international relations, yet did not have a formal understanding of the reified concept of multilateralism. Bridging this gap between the consensual sphere of the public’s ideals based on social representations of cooperation and conflict and the reified sphere containing political narratives of multilateralism is a key step to longer-term post-pandemic recovery. A first step will be further studies into how an ideal of a border-free world can reconfigure political resistance to xenophobic populist re-bordering.
The Effect of Intergenerational Parenting Mode on Children’s Outdoor Activities: A Case Study of Downtown Shanghai Communities
Authors: Tang Pai , Yang Dan , Dong Nannan
Published: October 2023

The miniaturization of Chinese urban families has led to an increasing trend of “intergenerational parenting” among urban families. However, the influence of different types of caregivers on children’s outdoor activities remains unclear. This study aimed to investigate the impact of various accompanying modes used by different caregivers on the outdoor activities of preschool children. The research employed mixed methods, including questionnaires and interviews, and focused on the intergenerational relationship in communities in Shanghai, China. The findings of this research reveal that caregivers from different generations adopt distinct accompanying modes when engaging in children’s outdoor activities. Grandparents tend to prioritize protection, while parents prefer to spend more time playing with their children. These preferences can be attributed to the division of labor within families and the social and physical environment of the communities in urban central areas. By shedding light on the intergenerational dynamics and caregiving approaches, this study provides a unique Chinese perspective on understanding the factors influencing children’s outdoor activities and experiences.
Optimizing Urban Children’s Outdoor Play Spaces: Affordances, Supervision, and Design Dynamics
Authors: Tang Pai , Woolley Helen
Published: October 2023

This research explores how outdoor spaces play a crucial role in providing urban children with opportunities for physical activity and relaxation, together with the supervision of the children’s outdoor activities, particularly in densely populated areas with limited open spaces. Effective design of such spaces is vital for creating functional recreational areas within urban landscapes. Drawing from ecological perceptual psychology’s theory of affordance, this research examines the positive attributes of environmental affordances, specifically in Beijing’s central area. Using a children’s park as a case study, systematic observations are conducted to understand the park’s potential for diverse play opportunities and facilitating social interactions across age groups. However, the results reveal that the children’s park falls short of realizing its affordances fully, restricting available play opportunities. Specific play activities require distinct spatial arrangements influenced by children’s ages. Maternal supervision significantly affects children’s play experiences. The study also highlights grandparent involvement in overseeing children’s outdoor play, revealing differing supervision styles. In conclusion, while fixed play equipment and rubber carpet ground enhance engagement and safety, realizing the complete potential of children’s outdoor play spaces demands more inclusive design. The research underscores the intricate interplay between environmental affordances, adult supervision patterns, and children’s play experiences, paving the way for further exploration of these dynamic relationships, and contribute to build environmentally and socially sustainable urban environments.
Modelling Interrelationships of the Factors Impeding Community Engagement in Risk-Sensitive Urban Planning: Evidence from Sri Lanka
Authors: Geekiyanage Devindi , Fernando Terrence , Keraminiyage Kaushal
Published: October 2023

During the last two decades, global disasters have impacted over 5.2 billion people, with economic losses exceeding USD 2.97 trillion. This underscores the critical need for inclusive risk-sensitive urban planning (RSUP) that integrates community insights. Community-based disaster risk reduction (CBDRR) has demonstrated a potential reduction of up to 40% in mortality rates during disasters and cost savings in disaster response and recovery. However, research has shown that only 20% felt they are involved in decisions related to neighborhood planning, despite communities’ lived experience in surviving local hazards. This highlights a gap where practitioners dominate the development of mitigation and development plans, sidelining local perspectives. Using Sri Lanka as a case study, this study investigated the barriers to effective community participation in the decision-making of RSUP and thereby developed an interpretive logic model to establish an understanding of why they occur and how each barrier is interlinked. The data gathered from a sample of 44 experts and community representatives revealed 19 factors that impede community inclusion in the decision-making of RSUP in Sri Lanka. The Total Interpretive Structural Modelling (TISM) analysis adopted identified that the absence of legal provisions for inclusive development, political dynamics, and corruption are the most significant barriers. The Matrix Impact of Cross Multiplication Applied to Classification (MICMAC) further revealed that fewer financial provisions and the absence of an institutional framework for community engagement are the linking barriers to the other 17 barriers. This study not only extends the theoretical debate on barriers to community engagement for risk-responsive and equitable development but also helps urban planners, disaster management practitioners, and strategy policymakers focus on critical areas that need major reforms.
The Spatial Distribution and Impacts of Organic Certificates in Southwest China
Authors: Guo Haixia , Li Yike , Hou Meiting , Wang Xie
Published: October 2023

Organic farming is a good choice for agricultural development in southwest China under the trade-off between environmental protection and agricultural development. However, no researcher has investigated the current state and development of organic agriculture in southwest China. As a result, this paper explored the spatial structure of organic agriculture in southwest China by examining the distribution of organic certificates, as well as the environmental and socioeconomic impacts. The results show a dramatically uneven distribution of certified organics among different provinces, cities, and organic certificate types. On the province scale, Guizhou has the highest number (1174) and density (73.40 per 1010 m2) of organic certificates. On the city scale, Zunyi and Chengdu have the highest densities (218.77 and 342.52 per 1010 m2, respectively). Most of the certified organics are plants, accounting for 76.95%. The spatial distribution of organic farming is influenced by the interaction of several factors, including precipitation, temperature, GDP, highway density, gross agriculture output, agriculture machinery, and rural employed persons. However, the related and determinant factors of organic certificate distribution vary greatly across different regions, spatial scales, economic development levels, and agricultural development statuses. For the entirety of southwestern China, the factors significantly related to the distribution of organic certificates are GDP, highway density, gross agriculture output, agriculture machinery, and rural employed persons. However, these factors are GDP, gross agriculture output, agriculture machinery, and rural employed persons for Sichuan, and gross agriculture output and rural employed persons for Guizhou. Factors constraining the development of organic agriculture in regions with better economic and agricultural conditions are much fewer than in poorer regions. All of the nine selected variables, except global radiation, are significantly related to organic certificate distribution in regions with better economic conditions, while none of them had a significant correlation with organic certificate distribution in poorer regions. Furthermore, climate is no longer a constraint in regions with better agricultural conditions. These findings are of great significance for the development and research of organic agriculture in southwest China. The development of organic agriculture in southwest China requires consideration of both the combination of multiple factors and the stage of regional economic and agricultural development.