bluuebunny/crossref_metadata_2025 huggingface dataset:
Silver Nanoparticles Anchored 5-methoxy benzimidazol thiomethanol (MBITM): Modulate, Characterization and Comparative Studies on MBITM and Ag-MBITM Antibacterial Activities
Authors: Alheety Nuaman F , Majeed Abdulwahab H , Alheety Mustafa A
Published: October 2019

This research highlights the synthesis of novel organic molecule that is 5-methoxybenzimidazolthiomethanol (MBITM). This molecule is synthesized via the reaction of 5-Methoxy-2-mercaptobenzimidazole with formaldehyde in ethanol. This resulting molecule was characterized via Fourier-transform infrared spectroscopy (FTIR), proton-nuclear magnetic resonance (1H-NMR) and elemental analysis (CHN). Furthermore, MBITM is used as a base compound for preparation of one novel nano material by mixing it with silver nanoparticles (Ag NPs) that prepared by green method using Zizyphus spina christi L (Seder) leaf, via a simple chemical reaction. The resulting Ag anchored MBITM (Ag-MBITM) was characterized by FTIR, X-ray diffraction (XRD) and Energy-dispersive X-ray spectroscopy (EDX) measurements. All these characterization measurements establish that a stable MBITM can be electrostatically retained on the surfaces of the Ag NPs. MBITM, Ag NPs and their nano derivative were screened for their antibacterial activity against both types of bacteria Gram-positive (S. aureus) and Gram-negative (E. Coli). In general, E-Coli and S. aureus were exposed to be significantly inhibited by all these three compounds but with different inhibition levels. Besides, the study of the biological activity was adopted for the purpose of conducting a comparative study between the raw materials (Ag NPs, MBITM) and the nanomaterial (Ag-MBITM) resulting from their reaction. The results showed that nanosilver-organic hybrid had effectively developed the antibacterial ability for both starting compounds. It has also been observed that this hybrid has been particularly effective with respect to the gram-positive bacteria.
Antioxidant and Antibacterial activity of some 2-amino-1,3,4-thiadiazole Schiff’s bases
Authors: Ibrahim Dheyaa H , Saleem Ali J , Awad Adil A , Ahmed Hiba S , Shneshil Mustafa K
Published: October 2019

Some 2-amino-1,3,4-thiadiazole Schiff’s bases were prepared by the reaction of chloro-acetamide with some aromatic aldehydes and the product directly reacted with 2-1mino-5-phenyl-1,3,4-thiadiazole. The Schiff’s bases prepared were characterized by F.T-IR, 1H-NMR and 13C-NMR. The 2-amino-1,3,4-thiadiazole Schiff’s bases (1-6) were tested for their antibacterial activity against (S. aureus, S. epidermidis, E. coli and P. aeruginosa) by using disc diffusion method. The minimum inhibitory concentrations (MICs) of the compounds also calculated by agar streak dilution method. The bases prepared also tested for their anti-oxidant activity against Fe and Cu ions.
Synthesis, characterazition of some 2-mercapto-5-methoxy-1H-benzimidazole and test with some plant pathogenic fungi
Authors: Jebur Ihmood Kh. , Mohammed Ismail Salih
Published: October 2019

In this research the new starting material 2-mercapto-5-methoxy-1H-benzimidazole (S1) was synthesized by the condensation reaction of 4-methoxy-o-phenylenediamine and (1mmol) and carbon disulfide (1 mmol) in ethanol (10 mL) under basic condition we used potassium hydroxide as base which produced MBI with high yield and purity. 2-mercapto-6-methoxy-1H-benzimidazole(S1), which on treatment with hydrazinehydrate in ethanol yielded hydrazino-6-methoxy-2-mercapto benzothiazole (S2).Condensation of compound (S2) with 4-(N,N-di Methylbenzaldehyde yielded 4-[(N,N-di Methyl bezylidine) hydrazino)]- 6-methoxy -2-mercaptobenzothiazole (S3).The synthesized compounds were identified according to their physical properties, spectroscopic data (FT-IR, 1H-NMR, and 13C-NMR) The study showed three chemical compounds test S1, S2, and S3 to three concentration (100, 200, 300) mg/L.in inhibiting colony diameter of three phytopathoginc fungi Fusarium oxysporum fsp.watermilon,Macro phomina phasiolina and Rhizoctonia solani these compounds effectiveness of inhibition of S1 and to three concentrations of the high against growth of fungi, with an inhibitory rate of 89.39% While S2 was less effective at inhibition of 33.89%.
Preparation, Charecterazation of new nitro-chalcone azo ligands and their divalent ionic complexes
Authors: Mahdi Saad M
Published: October 2019

In this study two of nitro-chalcone azo ligands were synthesized via the reaction of the diazonium salt of amino p-nitro chalcone (pNAC) with the coupling components (4,5-diphenylimidazole &2-naphthol) respectively.The new ligands were charecterized with many identificational tools such (H1NMR,FTIR and UV-Visible) spectroscopies, in addation to (CHNO)analysis for ensurance of the preparation of these ligands.A UV-Visible study for the aqueous mixtures of three divalent metal ions (Co,Ni and Cu) were perfomed to determination of the (λmax) of their colored mixtures, also to confirm the solid complexes structures via the determination of the suitable mole ratio (M:L), that all complexes mole ratios were (1:2, M:L). The solid complexes were synthesized and characterized by the obvious techniques, indeed, to the complementary techniques such (electrical conductivity, magnetic susceptibility) for determination of the complex formula and suggested their geometries, from the whole results, octahedral geometry can conculded for nitro chalcone azo imidazole complexes with the observation of the ionic characteristic of its, while the second ligand complexes differ from tetrahedral for (Co & Cu) complexes and square planer for nickel complex.
Thermophysical properties in deep eutectic solvents with/without water
Authors: Al-Murshedi Azhar Y M. , Alesary Hasan F , Al-Hadrawi Rajaa
Published: October 2019

Physical properties of pure DESs and DESs mixed with different amounts of water were investigated by different techniques, including, rotational viscometer, Quartz Crystal Microbalance (QCM) and conductivity meter. In order to study heterogeneity of those liquids in nature. Analysis of these data showed some characteristics of heterogeneity, the extent of which depends on the number of hydrogen bond donors in the pure DES.
Microwave Assisted Synthesis Characterization and Study of Some Novel Chalcones Compounds Derived From Mefenamic Acid
Authors: Al-Hazam Hanan , Al – Shamkhan Zeki A , Al-Mayah Abdulelah A , Hraishawi Rawaa M
Published: October 2019

New chalcones compounds were synthesis by reaction mefenamyl chloride with P-aminoacetophenone to from N–(4-acetyl phenyl)-2-(2/3- dimethyl phenyl) amino benzamide(A). The chalcones produced by Claisen – Schmidt condensation of compound A with substituted benzaldehydes [(2,4-dihydroxy(1), 3-NH2(2) and 4- OMe (3)] in ethanollic KOH solvent by using microwave irradiation. The new compounds were characterized by M.P, TLC, CHN, FT-IR, 1HNMR and 13CNMR. The biological screening data of the synthesized compounds were also presented. The theoretical study by using AM1 semi-empirical method emphasizes these compounds exists as trans configuration.
Color Removal of Azor A Dye in Aqueous Solution by ZnO and Hydrogen Peroxide Under Solar Irradiation
Authors: Hussein Batool S , Shaker Waheeb Azal , Mohsin Yasir Asstabraq , Alshamsi Hassan A
Published: October 2019

ZnO nanoparticles was synthesized by sol gel process and investigated using XRD, FT-IR, SEM and AFM analysis. Advanced oxidation processes (AOP) have showed to be very effective in degradation of most pollutants in wastewaters. The effect of initial concentration for Azure A dye was studied for five concentrations from (1×10−5 M to 5×10−5 M). The photocatalytic degradation of the dye was observed spectrophotometically. Also, the study involved effect of initial dye concentration, hydrogen peroxide dosage, pH, temperature and photocatalyst dose under solar light. The study involved to find out the optimum operating conditions of the treatment processes. Generally it has been found that the rates of photodegradation of azure A dye was higher in presence of solar radiation and ZnO photocatalyst. Also, photocatalytic ZnO showed the importance with light lead to enhance the generation of hydroxyl radicals which drive the photo oxidation processes. The kinetic and the percentages of degradation were studied at different intervals for 60 min. The experimental results indicated that the maximum photodegradation 89% of Azor A dyes occurred with ZnO photocatalyst at acidic media. COD analysis of The photodegraded samples showed reduction in COD after 60 min.
Quantitative Structure-Activity Relationship Studies Of Amino Acids Conjugated 2-Amnio-Arylthiazole As Antifungal
Authors: Jalal Mahir A , Almalki Ziyad T , Radhi Wisam A
Published: October 2019

Thiazole derivatives as fungi-inhibitors belonging to 16 amino acids conjugated 2-amnio-arylthiazole was subjected computationally to quantitative structure-activity relationship (QSAR) analysis by optimization of chemical structures at minimum energy using molecular mechanics (MM+) theory and the semi-empirical molecular orbital (AM1) method. Correlation of their experimental inhibitory zones against three types of fungi, namely, Fusarium monoliforme, Aspergillus Flavus, and Aspergillus niger with obtained physiochemical parameters was carried out using multiple linear regression (MLR) analysis. As a result, there excellent out of 12 models were correlated with numerous descriptors having correlation coefficient rang (0.967-0.843). discriminant models were selected depending on their correlation coefficients (R2), Fisher ratios (F), and standard errors (S).These QSAR results and the probable pharmacophore features identified in this study offer important structural insight into designing novel amino acids conjugated 2-amnio-arylthiazole. Other 15 thiazole derivatives was proposed and it found that they are in good inhibitory zones.
Synthesis, Characterization, DFT calculation and Antimicrobial Activity of Co(II) and Cu(II) complexes with azo dye
Authors: Shamran Mohammed Hasan , Deepak Tripathi Vishwa , Aldin Darghouth Ala , mothhar Taif
Published: October 2019

In this study, the ligands based on azo dye of 3,6- diamino-10-methyl acridinium chloride (MAA) and its complexes of Co(II) and Cu(II) were synthesized and characterized successfully via mass, UV-Vis, and infrared spectroscopy and elemental analysis.The energies of HOMO and LUMO orbitals of the MAA ligand were calculated via density functional theory (DFT). The LUMO orbital of the MAA dye is appropriate to donate electron injection to the condition band of TiO2. The band energy of MAA dye was 1.8 eV which encourages using it a sensitizing dye in dye-sensitized solar cells. The MAA ligand exhibited interaction ability more than reacting material (3,6- diamino-10-methyl acridinium) according to results of click docking software. The MAA dye and its complexes exhibited attractive biological activity towards streptococcus and staph aureus.
The Synthesis of a Novel Azo Dyes and Study of Photocatalytic Degradation
Authors: Karim Sadiq A , Al-Gubury Hazim Y , Abd Alrazzak Nour
Published: October 2019

Six novel aromatic azo dyes were synthesized from 4-amino-2-nitrobenzoic acid with phenol, catechol, resorcinol, hydroquinone, salicylic acid and acetylsalicylic acid with very good yield. These azo dyes confirm by FTIR, CHNS and study the photo stability used TiO2 and ZnO. The photocatalytic degradation of novel aromatic azo dyes was investigated using TiO2 and ZnO as a catalyst under 125 watts UV lamp. A series of experiments was performed to reaching to the optimum condition of degradation. The photocatalytic degradation of azo dye follows the first order reaction. In all experiments photocatalytic degradation of the azo dye has been studied using different weight of TiO2 and ZnO suspended in an aqueous solution of azo dye irradiated by irradiated by 125–UV lamp in 100 cm3 a vessel reactor at room temperature with 10 cm3/min air bubble has been passed through the solution. In addition, all result was measured using UV-Visible spectrophotometer.
Synthesis of mannich and triazole derivatives for barbiturate derivatives with some analytical applications
Authors: Jabbar Hussain Bashaer , Thamer Salim Ahmed
Published: October 2019

Mannich bases and heterocyclic compounds derivatives were prepared by reacting Phenobarbital and diethylbarbiturate with benzaldehyde derivatives and morpholine in THF as solvent, in another hand triazoles derivatives prepared by reacting azides barbiturates with unsaturated compounds We did some analytical application prepared compounds for some All compounds were identified by using TLC, FTIR, melting point and H1NMR C13NMR spectroscopy
Removal of Antibiotic Tetracycline (TCs) from aqueous solutions by using Titanium dioxide (TiO2) nanoparticles as an alternative material
Authors: Aljeboree Aseel M , Alkaim Ayad F
Published: October 2019

In this work Titanium dioxide (TiO2) can be used as a sorbent for drug eliminate from industrial Water treatment, drinking water such as tetracycline (TCs). Several operational standards like primary drug concentration, large dose, pH, and the temperature of the solution by use of TiO2 in the adsorption of drug TCs. The best result of the percentage removal E% (51-87%), (59-94%) and (70-97%) at temperature (288to 323K).The removal rate decreased with an increase in drug concentrations from 93.706 to 60.227% and Raising the concentration of the pipette adsorbent enhances rising ratio of eliminate till fullness of the adsorbent. This study appears that the adsorption emphasize on mechanism of decomposition TiO2 applied as a substitutional, economical and environmentally harmless condenser for water purification.
Statistical evaluation of spectrophotometric determination of nickel(II) using Micelle-mediated extraction.
Authors: Tariq Zianab , Adnan Shaimaa
Published: October 2019

An organic reagent namely 2-(2-bromophenyl)imino)methyl)-4- (5,6-dimethylpyridin-2-yl)diazenyl)phenol was synthesized and used for the determination of nickel after preconcentration using Micelle-mediated extraction.In this precocentration method the ligand (2-(2-bromophenyl)imino)methyl)-4- (5,6-dimethylpyridin-2-yl)diazenyl)phenol was used as a chelating agent for the extraction of Ni(II), using the nonionic surfactant Triton X-114 medium. Uv-Vis spectrophotometry was applied for the quantitation of the analyte at λmax(650)nm. All factors affecting the extraction and determination of Ni(II) ion using micelle mediation extraction(e.g. pH, surfactant and reagent concentrations, incubation time and temperature) were executed by using a classical optimization, the calibration plot was subjected to the statistical evalution by using the analysis of variance (ANOVA), The method was applied to the determination of Ni (II) in invironmental samples.
Synthesis, morphological, structural and topological characteristics of carbon nanosphere derived from Iraqi diesel
Authors: Hussain Nadia B , Habeeb Alshamsi Hassan A
Published: October 2019

The objective of this study involves the synthesis of carbon nanospheres(CNS) from incomplete burning of Iraqi diesel.Also, the synthesized CNS were further treated with hydrochloric acid and sonicated for 1 hour to achieve modified CNS.The results reveal that the produced carbon nanoparticles much smaller than those from the conventional diesel combustion.CNS were characterized using FT-IR spectroscopy (FTIR), scanning electron microscopy(SEM), atomic force microscopy(AFM), X-rays diffraction(XRD), Energy dispersive x-ray microanalysis (EDX), Brunauer-Emmett-Teller (BET) and Thermo gravimetric analysis (TGA). FTIR spectra showed a change is in nature of functional groups and exiting of adsorbed impurities on surfaceof synthesized carbonic materials. The results obtained from of SEM and XRD show a similar structure and morphology of the new materials. Thermal gravimetric analysis (TGA) analyses indicate a gradual weight loss in the temperature range from 0°C to about 700°C.AFM and BET analysis indicted a similar topology and surface area values of both materials. EDS analysis exhibited that carbon was the main product of diesel combustion.
Synthesis, Characterization, and Antibacterial activity of some new Bis β-Lactam Derivatives from Folic acid
Authors: Abdul wahab Alkuwaity Eman , Kadhim Oleiwi Zeyad
Published: October 2019

Firstly, two Schiff bases have been prepared by condensation reaction of folic acid with (4-chloroacetophenone and 4-hydroxyacetophenone) in ethanol. Then the resultant compounds (1,2) react with another Schiff bases by reacting of 4-bromobenzaldehyde with an amino acid (Phenylalanine, Tryptophan, Tyrosine, and Histidine) to give bis-β-lactam rings. The structures of target compounds were characterized by many spectroscopic methods including NMR, FTIR technique and their melting points. The β- lactam derivatives were checked and examined for antibacterial activity.
The expression of Interlukin 2(IL-2), Interlukin 8(IL-8) and Interlukin 6(IL-6) in patients with oral and oropharyngeal squamous cell carcinoma in Basrah city (A case control study)
Authors: Al-mahfoud Maha M , AlSaimary Ihsan E , Al shawi Ali A
Published: October 2019

The first part of the digestive tract is the oral mucosa, which exposed to different exogenous toxins, long period of exposure could lead to malignant changes/tumors. One of the prevalent cancers of the body is Oral squamous cell carcinoma, oral cancer sometimes may be resemble benign lesions in the mouth therefor, the diagnosis may be not easy clinically. There appear the importance of serum cytokines in distinguishing different pathologies.Aims: The aim of this study was to identify the role of serum Interlukins (IL2, IL6, IL8) in the early detection and pathogenesis of oral and oropharyngeal squamous cell carcinoma. Patients and Methods: This study included 20 patients with oral and oropharyngeal squamous cell carcinoma were clinically diagnosed and then confirmed by histopathological examination and 26 healthy control. Age, gender, as well as the level of interlukins in the serum of patients and healthy control were measured, in addition clinical signs and the site of lesions were recorded for patients group. Results: There were 20 new cases of oral and oropharyngeal squamous cell carcinoma in Basrah from September 2015 to December 2016, 12 in men and 8 in women. 26 healthy control individuals 17 men and 9 female were included in this study. Cancer at all oral sites affected men more than women. The Tongue is the most frequent site. The level of serum Interlukins (IL2, IL6, IL8) in patients group were much more than those in healthy control group.
Autoantibody Profile in Systemic Lupus Erythematosus Patients
Authors: Hafedh Abbas Ali , Krikor Melconian Alice , Hussein Ad’hiah Ali
Published: October 2019

Systemic lupus erythematosus (SLE) is an autoimmune disease, in which the etiology is not well-understood; however, interactions between environmental and genetic factors in predisposed individuals have been recognized. As a consequence, immunological alternations occur and immune cells are involved, especially T and B lymphocytes that are activated to produce different immune components. Among these components are autoantibodies that react with self-antigens aside from non-self-antigens due to the proposed theory of molecular mimicry. Accordingly, the current study was designed to examine the profile of different autoantibodies in SLE patients by using the indirect membrane based enzyme immunoassay for the quantitative measurement of IgG class antibodies.Subjects:Sixty-four SLE patients (32 arthritis and 32 nephritis patients) and 32 healthy subjects (control) were enrolled in the study, and their sera were tested for anti-nucleosome, anti-histone, anti-smD1, anti-PCNA, anti-PO, anti-SS-A/Ro-60, anti-SS-A/Ro-52, anti-SS-B/La, anti-CENP, anti-SCI-70, anti-U1snRNP, anti-AMA-M2, anti-Jo-1, anti-PM-SCI, anti-Mi2 and anti-Ku autoantibodies in order to evaluate the autoimmunity status in SLE patients.Results:The sera of control subjects were negative for these antibodies; therefore, the comparisons were limited to the two groups of SLE patients; arthritis and nephritis. The highest percentage of seropositive arthritis patients was observed for anti-SS-A/Ro-60, anti-CENP and anti-U1snRNP antibodies (100.0%), while the lowest percentage was recorded for anti-Jo-1 antibody (15.6%). For nephritis patients, anti-U1snRNP antibody (87.5%) was also observed to have the highest percentage, and anti-Jo-1 antibody (3.1%) also recoded the lowest percentage. However, four autoantibodies (anti-PCNA, anti-SS-A/Ro-60, anti-SS-B/La and anti-CENP antibodies) showed different profiles in arthritis and nephritis SLE patients. They showed a significant increased percentage in arthritis patients compared to nephritis patients (anti-PCNA: 87.5vs. 50.0%,p= 0.003; anti-SS-A/Ro-60: 100.0vs. 81.2%,p= 0.02; anti-SS-B/La: 75.0vs. 43.8%,p= 0.02; anti-CENP: 100.0vs. 43.8%,p= 0.001).Conclusion:These findings suggest the diagnostic potential of autoantibodies as early markers for SLE development.
The toxicity of phenolic compounds to some plants in the cumulative loss of the adult stages of domestic flies. Musca domestica (Diptera:Muscidae)
Authors: Mohammed Abed Falhi Al-Murmidhi Majedah , Ridha Anoun Al - Hasnawi Mohammad
Published: October 2019

The toxicity of phenolic compounds derived from Citrulls colocynthis, Rubus sanctus, and Lycium barbarum was tested in some aspects of the domestic fly. The results showed that the extract of phenolic compounds of al-Hantal plant achieved the highest percentage of eggs loss of 85.44% in the concentration of 20 mg / ml followed by the extract of the plant of the grouper with a loss of 76.44% and finally the blackberry plant with a loss of 63.94% with the same concentration compared to the control of 18.43 and 16.60 14.76% for the plants listed respectively. As for the effect of the phenolic extract on the destruction of the three larval stages, the effect was clear. The highest mortality rates for larval stages were achieved by exposure to the phenolic extract of all plants and in the concentration of 20 mg / ml.
Effect exposure of non-specialist tissue to ultraviolet treatments on gene expression and growth under cold stress in some breeds of beans
Authors: Kh Jabbar M
Published: October 2019

the experiment was conducted in laboratories of institute of genetic engineering and biotechnology of higher studies – university of Baghdad and college of sciences – university of Babylon, to study five breeds of beans to different mutation treatments on shoot apexes and its growth under low heat environments to product new winter lines, the irradiation of shoot apexes was after cut off it from shoots, ultraviolet radiation was used in three wavelengths (220,320 and 400 nm) interaction with two exposure periods (2 and 4 hours per day). the study traits were: Number days to 100% flowering - biological weight– weight of plant seeds, randomized complete block design was used with three replications, the results were indicated that B2 gave less number of days, higher biological and seeds weight while B1,B4 and B5 could not complete growth under W2,W3 treatments with different exposure periods, all breads gave flowering, biological and seeds weight with its variation at P1W1 and P1W2 treatments, the atpA gene was active in all breeds in P1W1 and P1W2 treatments while B2 and B3 were expressed gene in all treatments. From the results, there were new lines of beans added to winter plants as new crop was entered into food diversity program in Iraq.
Study role of hematological and leptin biomarkers in human infected with Entamoeba histolytica parasite
Authors: Khteer Al-Hadraawy Saleem , Hussein Abod Al-Khafaji Khama’al , Deqeem Fahim M. , Jawad Kadhim Naser
Published: October 2019

The study was conducted on 58 out patients with Entamoeba histolytica parasite and 28 healthy people as control group to determine the influences of infected on levels of iron, ferritin and leptin in patients infected with E. histolytica in compared with healthy group. Who have visited Al-Sadder medical city and Al- Hakeem Hospital in Al- Najaf Province during the period from January till August 2016. This disease diagnosis by using the wet amount microscope for stool from patients. The results showed significant decrease (P<0.05) iron and ferritin in E. histolytica infection patients in compared to control group. Also the results showed significant decreased (P<0.05) in serum leptin of patients with E. histolytica in compared to control group.
Assessment Of Visfatin Level In Patients With Coronary Heart Disease
Authors: Rajh Hamza Al-Kraity Wijdan , Jawad Murtadha M
Published: October 2019

In the current study, seventy of patients were divided into three study groups: acute myocardial infarction (AMI) patients group included 21 subjects, unstable angina (UA) patients group included 23 subjects and stable angina (SA) patients group included 26 subjects, the control group was composed of 20 healthy. The samples were collected from the Coronary Care Unit (CCU) in Al-Sadder Teaching Hospital in Al-Najaf province/Iraq, during the period from September till December 2018. The ages of patients and control ranged from 30 to 69 years old, Each patients was divided into subgroups according gender, type of disease, and age.The current study indicated a significant increase (P<0.05) in serum VF level of CHD compared with healthy group. The results also revealed a significant increase (p<0.05) in serum V F the level in AMI group as compared with UA group and SA group. The current study indicated no significant differences (p>0.05) in serum level between males and females of CHD patients. The results also indicated when compared with (30-39y), (40-49y), (50-59y) and (60-69y ) of CHD, a significant increase (p<0.05) in serum level VF there was a significant increase (p<0.05) among different age.Conclusion: The present study concluded that Visfatin level were good marker for detection and diagnosis of coronary heart disease in male and female patients.
Growth and productivity of Onion (Allium cepa L.) as influenced by set size and spraying with Nanocarbon.
Authors: Ali Abdul-Ameer Maha , Almousawy Nada A
Published: October 2019

A field study was conducted in Diwaniyah city at the winter season 2017-2018 for studying the effect of the set size and Nanocarbon compounds on the growth and yield of onion plant Allium cepa L. three set sizes (small, medium and large ) and two Nanocarbon concentrations(0 and 500 mg / L) were used in the experiment. A range of vegetative traits including plant height and number of leaves, total leaf area, chlorophyll rate and number of roots were studied. In addition to yield characteristics that includes number of bulbs, fresh and dry weight and productivity. The results showed that set size and Nanocarbon compounds significantly affected the vegetative growth of the studied plants. plants planted with large sets and treated by 500 mg/L Nano-carbon scored the best results for plant height, chlorophyll rate and leaf area. In which, it recorded the highest plant height (70.1 cm), and the maximum chlorophyll content (80.18 mg/ml), and the maximum leaf area (5.21 cm2), compared to control treatment which scored (26.5 cm), (47.59 mg/ml), (2.21cm2) for the same parameters above. The number of leaves/plants increased with the increase in set size while it decreased when treated by all Nano-carbon concentration. The large set gave the highest number of leaves/plants when not treated by Nanocarbon as it reached 8.5 leaves, in comparison with those treated by Nanocarbon and the comparison treatment which reached 7.1and 7.5 respectively. The plants planted with medium sets and treated by (500 mg/L) of Nanocarbon gave the largest number of roots/plant reached 90.1 root, compared to control plants, which have 68 root only. large set plants treated by 500mg/L. carbon nanotubes increased The number of double bulbs, fresh weight, dry weight and productivity increased to 7.9, 31.01g, 9.0 g and 70.017respectively in compare to control plants which scored 1.8, 27.59g, 2.531g and 64.636 for the same parameters respectively.
Molecular detection of CTX-M extended spectrum beta-lactamase among carbapenem- resistant Klebsiella pneumoniae from Al-Hillah Teaching Hospital environment, Babylon Province, Iraq
Authors: Moeen Abbas Fatima
Published: October 2019

This study analyze the prevalence of bla
CTX-M containing Klebsiella pneumoniae. During the period from October, 2017 to the end of January, 2018, a total of one hundred swab samples were collected from environment of Al-Hillah Teaching Hospital / Hillah city. Thirteen (13%) isolates were identified as K.pneumoniae. All K.pneumoniae isolates were subjected to antibiotic susceptibility testing using Kirby-Bauer disk diffusion method. Higher resistance rates were observed for penicillin antibiotics (ampicillin and cloxacillin) with resistance rate of (84.61%) and (69.23%), respectively. Extended spectrum beta-lactamase (ESBL) production was assayed phenotypically using disk combination method. Five (38%) isolates were screen-positive. Carbapenem resistance was detected in 2 isolates of K.pneumoniae, these were checked further by Polymerase Chain Reaction (PCR) method for the presence of bla
CTX-M gene, 1 (50%) isolate gave positive result.
The Impact of Genetic variation at TNF-α -308 G/A on their serum production and severity of Asthma disease
Authors: Darweesh Mayyada F , Kadhem Esraa J
Published: October 2019

Asthma is a chronic respiratory disease leading to morbidity and impaired quality of life worldwide. TNF-α cytokine responsible for the smooth muscle activation and late-phase airway hyper responsiveness. So this study aimed to investigateTNF-α polymorphisms situated at positions –308 G/Awith their serum level and asthma severity. A case–control study included 40 asthmatic patients 16 females and 24 males and matched with age and gender 40 healthy subjects as a control group. Blood samples were collected from both groups, genomic DNA was extracted from peripheral blood for further molecular study. The study appeared that TNF-α-308 G/A significantly (P<0.05) high frequencies of homozygous AA, and A allele carrier in asthmatic patients, in contrast homozygous GG genotype and G allele appear at low frequency with patients. The data showed that genetic variants of theTNF-α affect the TNF-α production and associated with the severity of asthma, AA and AG genotype associated with high serum level (58.2 pg/ml and 49.5 pg/ml) respectively and GG genotype associated with low serum level (24.06pg/ml).The study confirmed the distinct role of TNF-α in cases of asthma compared to healthy controls with significantly higher level among patients, mean serum level for patients and control were (50.3 and 5.88 ) pg/ml respectively. Also, mean serum levels of TNF-α were significantly higher in asthmatic patients that suffering from allergic rhinitis(65.39 pg/ml) compared to non-rhinitis patients (34.97pg/ml). In conclusion,TNF-α-308 GG, genotype contribute to the predisposition of asthma and there was significant association among genotype of this gene and level of cytokine as well as development of the disease. Also observed an association between this gene and serum level of cytokine. AA, AG genotype and allergic rhinitis consider a risk factor of asthma and progress the disease.
Effect of Silica Nanoparticles on level Cyp19a1and Cyp17a1genes in Male Rats
Authors: Allawi Luaibi Al-khauzay Hawraa , Mohammad Hasson Al-Husseini Alaa
Published: October 2019

The current study has been designed to evaluate the effect of silica Oxide nanoparticles (SiO2NPs) exposure on the reproductive performance of male rats. Forty adult
Wistar albino
male rats ( aged 60-70 days with body weight 130-140 g), were randomly divided into four equal groups, 10 rats per group: control group were received physiological saline, and three treated groups were administered by gavage at dose (1 mg, 10 mg and 100 mg/kg Body weight) of SiO2NPs suspension daily for 22 days, to study the effect of treated groups with silica nanoparticles on the expression of mRNA for Cyp19a1and Cyp17a1genes in testis by using qRT-PCR technique. Experimental results obtained from SiO2NPs treated male rats at the molecular level, the results appear that there is a significant (p<0.05) decrease in expression level of Cyp19a1and Cyp17a1genes in testis tissue for groups treated compared to control group.
Lipid Profile Survey in an adults in An-Najaf/Iraq: A cross-Sectional study
Authors: Al-Koofee Dhafer A F , Mohammed Ismail Jawad , Algenabi Abdulhussein A
Published: October 2019

The idea for this work was to assess the frequency of lipid profile in patients at the clinical chemistry laboratory of Al-Sader Medical city in An Najaf/Iraq from January to April 2018. The current work was conducted in clinical chemistry laboratory, Al-Sader medical city, An-Najaf, Iraq after institutional ethical approval and informed, to describe an observational and analytical study of outpatients, between January 2018 and April 2018. A total outpatient number of 820 who were visit the medical city for either check up or complain from an abnormal health state were involved in the this study. Age and sex of patients were ⩾ 18 years ( 404male, 414 female). Triglyceride levels and HDL –c levels were associated with gender and age not associated with occupational class or deprivation category. This contributed to the prevalence of hyperlipidemia. The findings of this study hyperlipidemia affects on the large number of Najaf population. This study suggest the variations in lipid profile association with gender and age may be largely attributed to potentially modifiable factors such as obesity, physical activity and dietary intake.
Seroprevalance of ToRCH Infection in pregnant women in Basra city Southern of Iraq
Authors: Dawood Ahmed H
Published: October 2019

TORCH infection (Toxoplasma gondii, Rubella virus, Cytomegalovirus, Herpes simplex virus) causes severe consequences in child in ongoing pregnancy. The goal of the study was to determine the rates of infection of the toxoplasmosis and viruses that cause abortion in pregnant women. Among 50 adult female serums From the date of 2018 –Jan-10 to 2018-Oct-12. The total number of samples of Qurnah 25 number of positive infection 15 and the infection estimated 15/25 Which accounted for 60%.while the infection rate in Abi al-Khasib district of the total 25 samples of the number of infection 8 and estimated the proportion of infection 8/25, which constitutes 32% of the toxoplasmosis. The results of the current epidemiological study confirmed that the viruses that cause abortion in the Qurnah& Abe al-Khasib district of Rubella were 4of the 50 blood samples (serum) showing that the percentage of infection is4/50, which is 8% and the rate of infection of Cytomegalovirus 8/50 which is 16% The results showed that herpes virus was more infected than the virus above 24/50 and that the percentage was estimated to be 48%. These results indicate that the most common percentage among pregnant women Toxoplasmosis& herpes virus Cytomegalovirus, Rubella).( 60%, 48%, 16%, and 8%, respectively.
Investigation of toxoplasmosis in sheep in Al-Diwaniya city by using modern technique
Authors: Al-Shaibani Khaled Thamer Matar , almaeahi Asifa matrood yassin , Sami alwan Alsheabani Marwa
Published: October 2019

Toxoplasma gondii, it’s not limited to the disease in sheep on the abortions and loss of births only, but beyond the transfer of infection to humans through consumption of sheep not cooked well meat infected with parasite, its considered one of the important sources of infection human with this disease, T. gondii were diagnosed in 80 blood samples of sheep at Al- Diwaniya city by using latex test and molecular diagnosis with Polymerase Chain Reaction depending of presence B1 gene of T. gondii, the results showed that antibodies were detected in 13 samples out of 80 (16.25%). of sheep since significant(P⩽ 0.05) in highest titration indicated at 1/80 (38.17%), while the lowest titer indicated at 1/40 (10.09), the results of Polymerase chain reaction for detection of B1 gene in blood of sheep showed that (7 of 80)(8.75%) of positive samples from the sheep and (73 of 80) negative samples. It had been concluded that sheep were found infected with T. gondii with possible transmission this disease to human and extends to public health through the consumption of infected meat that facilitates transmission of the disease to humans.
Qualitative and Quantitative Detection of Some Bacterial Vaginosis Pathogenic Markers Using Real-Time PCR
Authors: Al-Taweel Rana S , Naher Habeeb S , Albeldawi Abdaladeem Y
Published: October 2019

Bacterial vaginosis is a vaginal condition characterized by an abnormal vaginal discharge due to an overgrowth of normal bacteria in the vagina. Women with bacterial vaginosis also have fewer than the usual population of vaginal bacteria, i.e lactobacilli. In this study vaginal samples from 75 women were taken to detect and quantify two putative positive-indicator of bacterial vaginosis namely,Atopobium vaginaeandMegasphaeratype1, in addition to a negative-indicator of bacterial vaginosis (Lactobacillus crispatus) using real-time polymerase chain reaction technique. The prevalence of bacterial vaginosis was 34.66% according to scoring system, the most dominant species in patients and in women without the syndrome wasL. crispatus(80.76 and 79.59% respectively), but its concentration of16S rRNAgenes was significantly higher in bacterial vaginosis negative women (2.8×108vs. 4.74×107copy/swab). In bacterial vaginosis patients, the highest rDNA concentration was forMegasphaera1 (3.10×10816S rRNAcopy/swab).
Detection OF β- lactamase genes in clinical Klebsiella pneumonia strains isolated from hilla hospitals
Authors: Kadhim Kadher Marwa , Mohamme Jarallah Eman
Published: October 2019

This study aimed for isolation and identification of Klebsiella pneumonia, from clinical sample and from different hospitals in Hilla city. during the period from April to October 2017. 122/1056 (12%) isolates were belonged to Klebsiella pneumonia were collected. Growth on blood agar, MacConkey agar and Eosin methylene blue agar was identified by cultural, morphological and biochemical tests and confirmed by VITEK 2 system. The results showed that all the tested isolates were resistant to Ampicillin and Amoxicillin 122(100%), while 119(98%) for penicillin, whereas 100(82%) for piperacillin. Resistance to other drug classes varied among the isolates, a higher resistance was also detected with 95(78 %) to cefotaxime 99(81%) ceftazidime, 94(77%) to ceftriaxone and 92(75%) to ceftriaxone, The resistance to quinolones, nalidixic acid, ciprofloxacin and levofloxacin was detected 57(47%), 39(32%), 35(29%), respectively.majority of isolates 42 (34.4%) were obtained from sputum samples this result, 34 (27.8%) from urine, 17 (14 %) from stool, 15 (12.2 %) from burn, 8 ( 6.6 %) from vagina, 4 (3.3%) from wound, 1 (0.8%) from blood, 1 (0.8%) from ear and 0 (0 %) from both eye and throat. High prevalence rate for MDR, XDR and PDR in Merjan Teaching Hospital. The proportion of the patients from Merjan Teaching Hospital 27 (35.5%), 16 (44.4%) and 1 (50%) respectively. Detection of β - lactamase genes (blaCTX-M-9, blaCTX-M-10, and blaNDM-1) was performed by the conventional PCR technique. The results revealed that 36 isolates analyzed, blaCTX-M-9 12 (33.3%), blaCTX-M-10 14 (38.9%), and blaNDM-1 (0%).
Changes of gene expression profiles of infants inflammatory genes after Rota virus infection.
Authors: AL-Ghazal Abdulrhem T , Abdulghani Musto Mohammad
Published: October 2019

Rotavirus is a medical important pediatric pathogen causes severe gastroenteritis in infants, leading to 450,000 deaths globally each year. RT-PCR-Array technique analysis is a powerful method to determine global profiles of gene expression in cells and tissues after viral infections. Using this analysis, the Ct values of 14 immune inflammatory genes were subjected to determine their gene expression rates compared with Ct values of control (non-infected persons). Five housekeeping genes were used as internal control genes. All mRNA transcripts of these genes were upregulated (from 2.2974 fold change for CCL3, CCR1, CCR3, CXCL2, IFN-a, IFNA-g, IL1B, IL1R and 21.1121 fold change for TNF-b) after infection with rotavirus. This results revealed that rotavirus infection increase the gene expression rate of 14 inflammatory genes and lead to high impact on key immune pathways in infected infants.
Investigating Some Of Blood Parameters In Women With β-Thalassemia
Authors: AlAthari Rasha A , Mahdi Wijdan T
Published: October 2019

The current study was conducted in the Department of Life Sciences - Faculty of Science - University of Qadisiyah in cooperation with Maternity Hospital and Educational Children and the Center for Hematology in Diwaniyah for the period from 1/10/2017 to 1/4/2018. Fifty (50) samples were taken from women aged 15 to 35 years, and were divided into two groups: 30 women with thalassemia (experimental group) and 20 women without thalassemia (control group). Two (2) milliliter were taken from thalassemia-treated women and 2ml from healthy women for the purpose of conducting blood tests for phytoplankton, blood groups and the level of ferritin in the body. The results of the present study showed a significant decrease (P <0.01) in all blood parameters, hemoglobin, blood corpuscular, corpuscular volume, mean corpuscular hemoglobin in corpuscular and mean corpuscular hemoglobin contraction, compared with control group. Chemo-biological tests like ferritin testing confirmed significant ferritin increase by P<0.01 for the experimental group compared to the control group.
Histopathological study of Human Papillomavirus (HPV) in breast cancer patients
Authors: Al-Badry Bushra J , Talib Al-Muswie Rana , Jameel Sameera
Published: October 2019

A large number of epidemiological studies have evaluated the association between Human papillomavirus infection and breast carcinoma, but the results have been inconsistent.Numerous types of cytopathic effect are distinct in living cultures, but fixation and staining of the cells is essential to realize appearances of viral infection such as inclusion bodies. Identifying cytopathic effect and using it as a investigative tool needs much practice in observing stained section of numerous cell types. The present study aimed at diagnosing the histological changes in the malignant breast tissue, which caused by the HPV and comparing them with malignant breast tissue caused by other factors in order to find a way to diagnose the virus through the histopathic effect. A total of 100 samples was included in the study, 20 from benign tumor and 80 carcinomas, including different histopathological change. HPV was detected by using the conventional PCR. The breast cancer sections were examined for the expression of the effect of Human papillomavirus. In separate series the results of using formalin-fixed, paraffin -embedded sections were compared. Overall prevalence of HPV in malignant breast tissue was difference, we found that the histopathological characteristics of HPVassociated breast cancer are very different to breast cancercaused by other factors. Specifically, we showed the presence of HPV in breast cancer associates with the adenocarcinoma, The virus caused more activity in the tumor. Tumor is acinar carcinoma with increased cellularty and proliferative, While, in none infected with virus, there is no increased in cellularty or acinar, in some arease there was dilution of ducts.The observations indicate a likely causal role of HPV in causing a different histopathological change than breast cancercaused by other factors, This helps in the diagnosing of the HPV through cytopathic effect.
Study of some properties and antibacterial activity of (Alhagi maurorum Medik) collected from the desert of Al – Najaf
Authors: Abdulbary Meison
Published: October 2019

In traditional medicine, Alhagi maurorum is one member of medicinal plants which is used for many diseases therapy, that it is used as diuretic and Kidney stones treatments. This study was founded to identify some morphological characters and antibacterial activity of methanolic extract of A. maurorum. The morphological properties studied with macro and microscopical examination while the antibacterial activity by screening with well diffusion method against Bacillus cereus and Proteus mirabilis. The results showed that the plant is shrubby herb with evergreen appearance and its leaves are small obovate shape and the polygonal epidermal cells covered with thick cuticle layer. Stomata are frequently Paracytic type in addition to a few anomocytic type with unicellular covering trichome and prismatic crystals. The plant did not showed antibacterial activity against studied bacteria ( Bacillus cereus and Proteus mirabilis ) with concentrations up to 200 mg / ml.
Dissemination of 14bp deletion/insertion gene polymorphism of Human Leukocyte Antigen class I (G) with recurrent Spontaneous abortion in Baghdad
Authors: Jamal Abdulkhaliq Roua , Taher Mohammed Sabaa , Mohammed Abdul wahhab Alkhateeb Hiba , Abdul-Hassan Abbas Ahmed
Published: October 2019

Recurrent spontaneous abortion (RSA) is a surprisingly common occurrence in various populations, risk factors associated with pregnancy losses are largely variable and often changes among different communities. This study was designed to determine the possible association between HLA-G 14bp insertion/deletion gene polymorphism with recurrent spontaneous abortion. Peripheral blood was collected from 210 women (180 women with recurrent abortion three or more abortions and 30 women with normal pregnancy to three or more birth and without any previous abortion) in the first trimester. Based on clinical examination and diagnostic laboratory findings of ELISA for TORCH test were selected ninety from 180 women with recurrent abortion in the current study were divided into three groups: group one included 30 women with recurrent abortion with sero-negative for TORCH test, group two also 30 women with recurrent abortion with ser-positive for anti-toxoplasmaantibodies, while control group included 30 women with a healthy pregnancy. In the current study not found any significant alteration between heterozygous and homozygous amongst three groups, also not found any implication between recurrent abortion and healthy pregnant in the field of alleles (+14bp insertion or -14bp deletion). The genotyping and alleles of HLA-G 14bp (insertion/deletion) were not give in to the hypothesis of connotation between HLA-G and recurrent spontaneous abortion.
Detection of Rhamnolipid Production in Pseudomonas aeruginosa
Authors: Shamaa Sarab al- , Bahjat Shababa
Published: October 2019

Large variety of microorganisms produce potent surface-active agents, biosurfactants, which vary in their chemical properties and molecular size. Rhamnolipids are naturally occurring glycolipids produced by Pseudomonas aeruginosa species of bacteria. The enormous diversity of biosurfactants makes them an interesting group of materials for application in many areas such as agriculture, public health, food, health care, waste utilization, and environmental pollution control such as in degradation of hydrocarbons present in soil. Their ability to reduce surface tension is a major characteristic of surfactants, which is the key ingredient used in detergents, shampoo, toothpastes. Four strains of Pseudomonas aeruginosa obtained from oil contaminated soil with diesel, kerosene or benzene using four detection methods: Blood hemolysis, Oil spreading technique, cetyl trimethylammonium bromide (CTAB) agar plate, and Emulsifying activity. Ten out of eleven strains tested demonstrated rhamnolipid production in one or more of the techniques used and with variable amounts, with Pseudomonas aeruginosa strain D and T displaying positive and high productivity in all methods used. A new modification of oil spread technique was performed in the present study as this method was found to be the most efficient, easiest, and reliable among the others.