bluuebunny/crossref_metadata_2025 huggingface dataset:
Low-Energy Laser-Driven Ultrashort Pulsed Electron Beam Irradiation-Induced Immune Response in Rats
Authors: Tsakanova Gohar , Babayan Nelly , Karalova Elena , Hakobyan Lina , Abroyan Liana , Avetisyan Aida , Avagyan Hranush , Hakobyan Sona , Poghosyan Arpine , Baghdasaryan Bagrat , Arakelova Elina , Ayvazyan Violetta , Matevosyan Lusine , Navasardyan Arpine , Davtyan Hakob , Apresyan Lilit , Yeremyan Arsham , Aroutiounian Rouben , Osipov Andreyan N. , Grigoryan Bagrat , Karalyan Zaven
Published: October 2021

The development of new laser-driven electron linear accelerators, providing unique ultrashort pulsed electron beams (UPEBs) with low repetition rates, opens new opportunities for radiotherapy and new fronts for radiobiological research in general. Considering the growing interest in the application of UPEBs in radiation biology and medicine, the aim of this study was to reveal the changes in immune system in response to low-energy laser-driven UPEB whole-body irradiation in rodents. Forty male albino Wistar rats were exposed to laser-driven UPEB irradiation, after which different immunological parameters were studied on the 1st, 3rd, 7th, 14th, and 28th day after irradiation. According to the results, this type of irradiation induces alterations in the rat immune system, particularly by increasing the production of pro- and anti-inflammatory cytokines and elevating the DNA damage rate. Moreover, such an immune response reaches its maximal levels on the third day after laser-driven UPEB whole-body irradiation, showing partial recovery on subsequent days with a total recovery on the 28th day. The results of this study provide valuable insight into the effect of laser-driven UPEB whole-body irradiation on the immune system of the animals and support further animal experiments on the role of this novel type of irradiation.
Conductive Polymeric-Based Electroactive Scaffolds for Tissue Engineering Applications: Current Progress and Challenges from Biomaterials and Manufacturing Perspectives
Authors: Marsudi Maradhana Agung , Ariski Ridhola Tri , Wibowo Arie , Cooper Glen , Barlian Anggraini , Rachmantyo Riska , Bartolo Paulo J. D. S.
Published: October 2021

The practice of combining external stimulation therapy alongside stimuli-responsive bio-scaffolds has shown massive potential for tissue engineering applications. One promising example is the combination of electrical stimulation (ES) and electroactive scaffolds because ES could enhance cell adhesion and proliferation as well as modulating cellular specialization. Even though electroactive scaffolds have the potential to revolutionize the field of tissue engineering due to their ability to distribute ES directly to the target tissues, the development of effective electroactive scaffolds with specific properties remains a major issue in their practical uses. Conductive polymers (CPs) offer ease of modification that allows for tailoring the scaffold’s various properties, making them an attractive option for conductive component in electroactive scaffolds. This review provides an up-to-date narrative of the progress of CPs-based electroactive scaffolds and the challenge of their use in various tissue engineering applications from biomaterials perspectives. The general issues with CP-based scaffolds relevant to its application as electroactive scaffolds were discussed, followed by a more specific discussion in their applications for specific tissues, including bone, nerve, skin, skeletal muscle and cardiac muscle scaffolds. Furthermore, this review also highlighted the importance of the manufacturing process relative to the scaffold’s performance, with particular emphasis on additive manufacturing, and various strategies to overcome the CPs’ limitations in the development of electroactive scaffolds.
The Mutation in wbaP cps Gene Cluster Selected by Phage-Borne Depolymerase Abolishes Capsule Production and Diminishes the Virulence of Klebsiella pneumoniae
Authors: Kaszowska Marta , Majkowska-Skrobek Grazyna , Markwitz Pawel , Lood Cédric , Jachymek Wojciech , Maciejewska Anna , Lukasiewicz Jolanta , Drulis-Kawa Zuzanna
Published: October 2021

Klebsiella pneumoniae is considered one of the most critical multidrug-resistant pathogens and urgently requires new therapeutic strategies. Capsular polysaccharides (CPS), lipopolysaccharides (LPS), and exopolysaccharides (EPS) are the major virulence factors protecting K. pneumoniae against the immune response and thus may be targeted by phage-based therapeutics such as polysaccharides-degrading enzymes. Since the emergence of resistance to antibacterials is generally considered undesirable, in this study, the genetic and phenotypic characteristics of resistance to the phage-borne CPS-degrading depolymerase and its effect on K. pneumoniae virulence were investigated. The K63 serotype targeting depolymerase (KP36gp50) derived from Klebsiella siphovirus KP36 was used as the selective agent during the treatment of K. pneumoniae 486 biofilm. Genome-driven examination combined with the surface polysaccharide structural analysis of resistant mutant showed the point mutation and frameshift in the wbaP gene located within the cps gene cluster, resulting in the loss of the capsule. The sharp decline in the yield of CPS was accompanied by the production of a larger amount of smooth LPS. The modification of the surface polysaccharide layers did not affect bacterial fitness nor the insensitivity to serum complement; however, it made bacteria more prone to phagocytosis combined with the higher adherence and internalization to human lung epithelial cells. In that context, it was showed that the emerging resistance to the antivirulence agent (phage-borne capsule depolymerase) results in beneficial consequences, i.e., the sensitization to the innate immune response.
Dissecting the Supramolecular Dispersion of Fullerenes by Proteins/Peptides: Amino Acid Ranking and Driving Forces for Binding to C60
Authors: Marforio Tainah Dorina , Calza Alessandro , Mattioli Edoardo Jun , Zerbetto Francesco , Calvaresi Matteo
Published: October 2021

Molecular dynamics simulations were used to quantitatively investigate the interactions between the twenty proteinogenic amino acids and C60. The conserved amino acid backbone gave a constant energetic interaction ~5.4 kcal mol−1, while the contribution to the binding due to the amino acid side chains was found to be up to ~5 kcal mol−1 for tryptophan but lower, to a point where it was slightly destabilizing, for glutamic acid. The effects of the interplay between van der Waals, hydrophobic, and polar solvation interactions on the various aspects of the binding of the amino acids, which were grouped as aromatic, charged, polar and hydrophobic, are discussed. Although π–π interactions were dominant, surfactant-like and hydrophobic effects were also observed. In the molecular dynamics simulations, the interacting residues displayed a tendency to visit configurations (i.e., regions of the Ramachandran plot) that were absent when C60 was not present. The amino acid backbone assumed a “tepee-like” geometrical structure to maximize interactions with the fullerene cage. Well-defined conformations of the most interactive amino acids (Trp, Arg, Met) side chains were identified upon C60 binding.
Compatibility between Rice Straw Fibers with Different Pretreatments and Ordinary Portland Cement
Authors: Xie Xiaoli , Li Hongbo
Published: October 2021

The compatibility between crop straw and Portland cement greatly restrict the application of crop straw in cement-based materials. In this study, rice straw fibers with different pretreatments were added to ordinary Portland cement (OPC), and the influence of different rice straw fiber (RF) content on the hydration process of OPC was measured using calorimeter tests. Additionally, compatibility between RF and OPC was evaluated using the inhibitory index. As a result, steam explosion treatment of rice straw removed most hemicellulose and post-treatment bleaching was used for delignification. As compared with the pure OPC, addition of RF inhibited the hydration of OPC, and the inhibition degree reduced with the increase in pretreatment degree of RF. The inhibitory index grade of different RF filled OPC (RF-OPC) samples is directly related to hemicellulose and lignin content. Compared with lignin, hemicellulose has a greater influence on cement hydration. Without considering the influence of other components, the RF-OPC samples with hemicellulose content of 1.54 wt.% reached the inhibitory index extreme grade, and the hemicellulose content of 2.05 wt.% led to the cessation of cement hydration. The inhibitory index of the samples with 2.05 and 0.85 wt.% lignin content is moderate and low grade, respectively. In addition, the results of XRD patterns and SEM images are consistent with those of heat of hydration. In terms of mechanical properties of cement-based composites with 10 wt.% rice straw fibers, pretreatment of fibers is beneficial to improving the fracture toughness of the samples.
The Correlation of Regional Microstructure and Mechanics of the Cervical Articular Process in Adults
Authors: Feng Huimei , Ma Yuan , Wang Stephen Jia , Zhang Shaojie , Li Zhijun
Published: October 2021

Purpose: Using micro-CT and finite element analysis to establish regional variation microarchitectures and correlation with mechanical properties of cervical articular facet trabecular bone to predict cervical spine security and material properties. Methods: A total of 144 cervical articular processes (each articular was separate to four region of interest (ROI), superior-anterior (SA), superior-posterior (SP), inferior-anterior (IA), and inferior-posterior (IP) regions) specimens with a volume of 5 × 5 × 5 mm3 were scanned by micro-CT, and allowable stress and other mechanical properties parameters in each region were calculated after mechanical testing, then the effectiveness was verified of finite element models by ABAQUS software. Results: Maximum and minimum values of C2–C7 articular processes and regions are C5 and C7 level, SA and SP regions for bone volume fraction (BV/TV) and trabecular thickness (Tb.Th), whose variation tendency is similar to the Young’s modulus, allowable stress, BMD, maximum force and strain. Between Young’s modulus and all microstructure parameters, especially between BV/TV, bone mineral density (BMD) and Tb.Th, had higher linear regression coefficients R2 = 0.5676, 0.6382, 0.3535, respectively. BMD and yield strength, BV/TV, and allowable stress also had better regression coefficients, R2 = 0.5227, 0.5259, 0.5426, respectively. Conclusions: The contribution of the microstructure and mechanical properties of the C2–C7 cervical spine to the movement of the cervical spine is different and has a good correlation and the effectiveness of the finite element model is also verified that we can correctly calculate the microstructure and mechanical properties of the cervical articular process to evaluate the stability and injury risk of cervical vertebrae by the established model.
Regeneration of Aluminum Matrix Composite Reinforced by SiCp and GCsf Using Gas Tungsten Arc Welding Technology
Authors: Łyczkowska Katarzyna , Adamiec Janusz , Dolata Anna Janina , Dyzia Maciej , Wieczorek Jakub
Published: October 2021

The main motivation behind the presented research was the regeneration of the damaged surface of composite materials. The testing of melting and pad welding of the composite surface by Gas Tungsten Arc Welding (GTAW) with alternating current (AC) were carried out. The material of investigation was an AlSi12/SiCp + GCsf hybrid composite made by a centrifugal casting process. The composite was reinforced with 5 wt.% of silicon carbide particles and 5 wt.% of glassy carbon spheres. The composites were investigated in tribological tests. It was found that there was a possibility for modification or regeneration of the surface with pad welding technology. Recommended for the repairs was the pad welding method with filler metal with a chemical composition similar to the aluminum matrix composite (ISO 18273 S Al4047A (AlSi12 [A])). The surface of the pad welding was characterized by the correct structure with visible SiCp. No gases or pores were observed in the pad welding; this was due to a better homogeneity of the silicon carbide (SiCp) distribution in the composite and better filling spaces between liquid metal particles in comparison to the base material. Based on the tribological tests, it was found that the lowest wear was observed for the composite surface after pad welding. This was related to the small number of reinforcing particles and their agreeable bonding with the matrix. The plastic deformation of the Al matrix and scratching by worn particles were a dominant wear mechanism of the surface.
Hydrothermal Aging of ATZ Composites Based on Zirconia Made of Powders with Different Yttria Content
Authors: Grabowy Marek , Wilk Agnieszka , Lach Radosław , Pędzich Zbigniew
Published: October 2021

The presented work concerns the development and investigation of three different grades of ZrO2 materials containing Al2O3 particles (ATZ-Alumina Toughened Zirconia ceramics with 2.3–20 vol.% of alumina). The zirconia powders containing 3 mol.% of yttria were synthesized by a precipitation/calcination method and fabricated from two different zirconia powders with different yttria content. Then, the selected ATZ composites (ATZ-B, ATZ-10 and ATZ-20) were prepared by means of conventional mixing, compacting and sintering at 1450 °C for 1.5 h. The phase composition, microstructure, relative density and basic mechanical properties were determined. Uniform microstructures with relative densities over 99% of theoretical density, hardness values between 12.0–13.8 GPa, flexural strength up to 1 GPa and outstanding fracture toughness of 12.7 MPa⋅m1/2 were obtained. The aging susceptibility of alumina toughened zirconia materials, as a consequence of hydrothermal treatment, was investigated. The aim of this study was to determine the influence of LTD (low temperature degradation) on the tetragonal to monoclinic phase transitions and on the flexural strength of hydrothermally aged specimens. The results were compared to those obtained for commercially available tetragonal zirconia-based materials containing 3 mol.% of yttria. This research shows that ATZ composites that have excellent mechanical properties and sufficient hydrothermal aging resistance can be attained and later used in technical and biomedical applications.
Investigation of Cation Exchange Behaviors of FAxMA1−xPbI3 Films Using Dynamic Spin-Coating
Authors: Yu Hyang Mi , Jeong Byeong Geun , Park Dae Young , Lim Seong Chu , Namkoong Gon , Jeong Mun Seok
Published: October 2021

In this study, we fabricated and characterized uniform multi-cation perovskite FAxMA1−xPbI3 films. We used the dynamic spin-coating method to control the cation ratio of the film by gradually increasing the FA+, which replaced the MA+ in the films. When the FA+ concentration was lower than xFA ~0.415 in the films, the stability of the multi-cation perovskite improved. Above this concentration, the film exhibited δ-phase FAPbI3 in the FAxMA1−xPbI3 films. The formation of δ-phase FAPbI3 disturbed the homogeneity of the photoluminescence spatial distribution and suppressed the absorption spectral bandwidth with the increasing bandgap. The precise control of the cation ratio of multi-cation perovskite films is necessary to optimize the energy-harvesting performance.
New Method for Preparing Small-Caliber Artificial Blood Vessel with Controllable Microstructure on the Inner Wall Based on Additive Material Composite Molding
Authors: Hu Junchao , Jian Zhian , Lu Chunxiang , Liu Na , Yue Tao , Lan Weixia , Liu Yuanyuan
Published: October 2021

The diameter of most blood vessels in cardiovascular and peripheral vascular system is less than 6 mm. Because the inner diameter of such vessels is small, a built-in stent often leads to thrombosis and other problems. It is an important goal to replace it directly with artificial vessels. This paper creatively proposed a preparation method of a small-diameter artificial vascular graft which can form a controllable microstructure on the inner wall and realize a multi-material composite. On the one hand, the inner wall of blood vessels containing direct writing structure is constructed by electrostatic direct writing and micro-imprinting technology to regulate cell behavior and promote endothelialization; on the other hand, the outer wall of blood vessels was prepared by electrospinning PCL to ensure the stability of mechanical properties of composite grafts. By optimizing the key parameters of the graft, a small-diameter artificial blood vessel with controllable microstructure on the inner wall is finally prepared. The corresponding performance characterization experimental results show that it has advantages in structure, mechanical properties, and promoting endothelialization.
1H NMR Combined with Multivariate Statistics for Discrimination of Female and Male Flower Buds of Populus tomentosa
Authors: Xu Bo , Wu Cui , Li Zhuojun , Song Pingping , Chao Zhimao
Published: October 2021

1H Nuclear Magnetic Resonance (1H NMR) combined with multivariate statistics was adopted to discriminate female and male flower buds of Populus tomentosa in the study. Samples of 11 female and 16 male flower buds of P. tomentosa were collected in Beijing, China. 1H NMR spectra were acquired on a 400 MHz spectrometer. In total, 30 chemical compounds were identified with standards and literature according to chemical shifts, peak areas, and multiplicity. Principal component analysis (PCA), hierarchical clustering analysis (HCA), and supervised orthogonal partial least squares-discriminant analysis (OPLS-DA) were applied to discriminate female and male flower buds. An apparent grouping trend (R2X, 0.809; Q2, 0.903) between female and male groups was exhibited with PCA and HCA. The two groups were also well discriminated with OPLS-DA (R2X, 0.808; R2Y, 0.976; Q2, 0.960). Combined with variable importance in projection (VIP) > 1.0 and p < 0.05 of OPLS-DA, it was found that the content of daucosterol, β-sitosterol, ursolic acid, and betulonic acid in male group was higher than that in female, which should be the key differences of chemical constituents in female and male flower buds of P. tomentosa. The study demonstrated that 1H NMR combined with multivariate statistics could be used to discriminate female and male plants and clarify differences, which provided a novel method to identify the gender of dioecious plants.
Potential Vasculoprotective Effects of Blackcurrant (Ribes nigrum) Extract in Diabetic KK-Ay Mice
Authors: Horie Kayo , Maeda Hayato , Nanashima Naoki , Oey Indrawati
Published: October 2021

Polyphenols are bioactive compounds found naturally in fruits and vegetables; they are widely used in disease prevention and health maintenance. Polyphenol-rich blackcurrant extract (BCE) exerts beneficial effects on vascular health in menopausal model animals. However, the vasculoprotective effects in diabetes mellitus (DM) and atherosclerotic vascular disease secondary to DM are unknown. Therefore, we investigated whether BCE is effective in preventing atherosclerosis using KK-Ay mice as a diabetes model. The mice were divided into three groups and fed a high-fat diet supplemented with 1% BCE (BCE1), 3% BCE (BCE2), or Control for 9 weeks. The mice in the BCE2 group showed a considerable reduction in the disturbance of elastic lamina, foam cell formation, and vascular remodeling compared to those in the BCE1 and Control groups. Immunohistochemical staining indicated that the score of endothelial nitric oxide synthase staining intensity was significantly higher in both BCE2 (2.9) and BCE1 (1.9) compared to that in the Control (1.1). Furthermore, the score for the percentage of alpha-smooth muscle actin was significantly lower in the BCE2 (2.9%) than in the Control (2.1%). Our results suggest that the intake of anthocyanin-rich BCE could have beneficial effects on the blood vessels of diabetic patients.
Progress on Noble Metal-Based Catalysts Dedicated to the Selective Catalytic Ammonia Oxidation into Nitrogen and Water Vapor (NH3-SCO)
Authors: Jabłońska Magdalena
Published: October 2021

A recent development for selective ammonia oxidation into nitrogen and water vapor (NH3-SCO) over noble metal-based catalysts is covered in the mini-review. As ammonia (NH3) can harm human health and the environment, it led to stringent regulations by environmental agencies around the world. With the enforcement of the Euro VI emission standards, in which a limitation for NH3 emissions is proposed, NH3 emissions are becoming more and more of a concern. Noble metal-based catalysts (i.e., in the metallic form, noble metals supported on metal oxides or ion-exchanged zeolites, etc.) were rapidly found to possess high catalytic activity for NH3 oxidation at low temperatures. Thus, a comprehensive discussion of property-activity correlations of the noble-based catalysts, including Pt-, Pd-, Ag- and Au-, Ru-based catalysts is given. Furthermore, due to the relatively narrow operating temperature window of full NH3 conversion, high selectivity to N2O and NOx as well as high costs of noble metal-based catalysts, recent developments are aimed at combining the advantages of noble metals and transition metals. Thus, also a brief overview is provided about the design of the bifunctional catalysts (i.e., as dual-layer catalysts, mixed form (mechanical mixture), hybrid catalysts having dual-layer and mixed catalysts, core-shell structure, etc.). Finally, the general conclusions together with a discussion of promising research directions are provided.
Modulation and Pharmacology of the Mitochondrial Permeability Transition: A Journey from F-ATP Synthase to ANT
Authors: Carrer Andrea , Laquatra Claudio , Tommasin Ludovica , Carraro Michela
Published: October 2021

The permeability transition (PT) is an increased permeation of the inner mitochondrial membrane due to the opening of the PT pore (PTP), a Ca2+-activated high conductance channel involved in Ca2+ homeostasis and cell death. Alterations of the PTP have been associated with many pathological conditions and its targeting represents an incessant challenge in the field. Although the modulation of the PTP has been extensively explored, the lack of a clear picture of its molecular nature increases the degree of complexity for any target-based approach. Recent advances suggest the existence of at least two mitochondrial permeability pathways mediated by the F-ATP synthase and the ANT, although the exact molecular mechanism leading to channel formation remains elusive for both. A full comprehension of this to-pore conversion will help to assist in drug design and to develop pharmacological treatments for a fine-tuned PT regulation. Here, we will focus on regulatory mechanisms that impinge on the PTP and discuss the relevant literature of PTP targeting compounds with particular attention to F-ATP synthase and ANT.
Structural Characterization of Unusual Fatty Acid Methyl Esters with Double and Triple Bonds Using HPLC/APCI-MS2 with Acetonitrile In-Source Derivatization
Authors: Horká Petra , Vrkoslav Vladimír , Kindl Jiří , Schwarzová-Pecková Karolina , Cvačka Josef
Published: October 2021

Double and triple bonds have significant effects on the biological activities of lipids. Determining multiple bond positions in their molecules by mass spectrometry usually requires chemical derivatization. This work presents an HPLC/MS method for pinpointing the double and triple bonds in fatty acids. Fatty acid methyl esters were separated by reversed-phase HPLC with an acetonitrile mobile phase. In the APCI source, acetonitrile formed reactive species, which added to double and triple bonds to form [M + C3H5N]+• ions. Their collisional activation in an ion trap provided fragments helpful in localizing the multiple bond positions. This approach was applied to fatty acids with isolated, cumulated, and conjugated double bonds and triple bonds. The fatty acids were isolated from the fat body of early-nesting bumblebee Bombus pratorum and seeds or seed oils of Punicum granatum, Marrubium vulgare, and Santalum album. Using the method, the presence of the known fatty acids was confirmed, and new ones were discovered.
Haematococcus pluvialis as a Potential Source of Astaxanthin with Diverse Applications in Industrial Sectors: Current Research and Future Directions
Authors: Oslan Siti Nur Hazwani , Tan Joo Shun , Oslan Siti Nurbaya , Matanjun Patricia , Mokhtar Ruzaidi Azli Mohd , Shapawi Rossita , Huda Nurul
Published: October 2021

Haematococcus pluvialis, a green microalga, appears to be a rich source of valuable bioactive compounds, such as astaxanthin, carotenoids, proteins, lutein, and fatty acids (FAs). Astaxanthin has a variety of health benefits and is used in the nutraceutical and pharmaceutical industries. Astaxanthin, for example, preserves the redox state and functional integrity of mitochondria and shows advantages despite a low dietary intake. Because of its antioxidant capacity, astaxanthin has recently piqued the interest of researchers due to its potential pharmacological effects, which include anti-diabetic, anti-inflammatory, and antioxidant activities, as well as neuro-, cardiovascular-, ocular, and skin-protective properties. Astaxanthin is a popular nutritional ingredient and a significant component in animal and aquaculture feed. Extensive studies over the last two decades have established the mechanism by which persistent oxidative stress leads to chronic inflammation, which then mediates the majority of serious diseases. This mini-review provides an overview of contemporary research that makes use of the astaxanthin pigment. This mini-review provides insight into the potential of H. pluvialis as a potent antioxidant in the industry, as well as the broad range of applications for astaxanthin molecules as a potent antioxidant in the industrial sector.
The Role of 20-HETE, COX, Thromboxane Receptors, and Blood Plasma Antioxidant Status in Vascular Relaxation of Copper-Nanoparticle-Fed WKY Rats
Authors: Majewski Michał , Juśkiewicz Jerzy , Krajewska-Włodarczyk Magdalena , Gromadziński Leszek , Socha Katarzyna , Cholewińska Ewelina , Ognik Katarzyna
Published: October 2021

Recently, the addition of copper nanoparticles (NPs) in a daily diet (6.5 mg/kg) was studied in different animal models as a possible alternative to ionic forms. Male Wistar–Kyoto rats (24-week-old, n = 11) were fed with copper, either in the form of carbonate salt (Cu6.5) or metal-based copper NPs (NP6.5), for 8 weeks. The third group was fed with a half dose of each (NP3.25 + Cu3.25). The thoracic aorta and blood plasma was studied. Supplementation with NP6.5 decreased the Cu (×0.7), Cu/Zn-ratio (×0.6) and catalase (CAT, ×0.7), and increased Zn (×1.2) and superoxide dismutase (SOD, ×1.4). Meanwhile, NP3.25 + Cu3.25 decreased the Cu/Zn-ratio (×0.7), and CAT (×0.7), and increased the daily feed intake (×1.06). Preincubation with either the selective cyclooxygenase (COX)-2 inhibitor, or the non-selective COX-1/2 inhibitor attenuated vasodilation of rat thoracic aorta in the NP6.5 group exclusively. However, an increased vasodilator response was observed in the NP6.5 and NP3.25 + Cu3.25 group of rats after preincubation with an inhibitor of 20-hydroxyeicosatetraenoic acid (20-HETE) formation, and the thromboxane receptor (TP) antagonist. Significant differences were observed between the NP6.5 and NP3.25 + Cu3.25 groups of rats in: dietary intake, acetylcholine-induced vasodilation, and response to COX-inhibitors. Copper NPs in a standard daily dose had more significant effects on the mechanism(s) responsible for the utilization of reactive oxygen species in the blood plasma with the participation of prostanoids derived from COX-2 in the vascular relaxation. Dietary copper NPs in both doses modified vasodilation through the vasoconstrictor 20-HETE and the TP receptors.
Natural Products for Pancreatic Cancer Treatment: From Traditional Medicine to Modern Drug Discovery
Authors: Kim Ahyeon , Ha Jiwon , Kim Jeongeun , Cho Yongmin , Ahn Jimyung , Cheon Chunhoo , Kim Sung-Hoon , Ko Seong-Gyu , Kim Bonglee
Published: October 2021

Pancreatic cancer, the seventh most lethal cancer around the world, is considered complicated cancer due to poor prognosis and difficulty in treatment. Despite all the conventional treatments, including surgical therapy and chemotherapy, the mortality rate is still high. Therefore, the possibility of using natural products for pancreatic cancer is increasing. In this study, 68 natural products that have anti-pancreatic cancer effects reported within five years were reviewed. The mechanisms of anti-cancer effects were divided into four types: apoptosis, anti-metastasis, anti-angiogenesis, and anti-resistance. Most of the studies were conducted for natural products that induce apoptosis in pancreatic cancer. Among them, plant extracts such as Eucalyptus microcorys account for the major portion. Some natural products, including Moringa, Coix seed, etc., showed multi-functional properties. Natural products could be beneficial candidates for treating pancreatic cancer.
Nanoencapsulation as a General Solution for Lyophilization of Labile Substrates
Authors: Vallerinteavide Mavelli Girish , Sadeghi Samira , Vaidya Siddhesh Sujit , Kong Shik Nie , Drum Chester Lee
Published: October 2021

Protein macromolecules occur naturally at the nanoscale. The use of a dedicated nanoparticle as a lyophilization excipient, however, has not been reported. Because biopolymeric and lipid nanoparticles often denature protein macromolecules and commonly lack the structural rigidity to survive the freeze-drying process, we hypothesized that surrounding an individual protein substrate with a nanoscale, thermostable exoshell (tES) would prevent aggregation and protect the substrate from denaturation during freezing, sublimation, and storage. We systematically investigated the properties of tES, including secondary structure and its homogeneity, throughout the process of lyophilization and found that tES have a near 100% recovery following aqueous reconstitution. We then tested the hypothesis that tES could encapsulate a model substrate, horseradish peroxidase (HRP), using charge complementation and pH-mediated controlled assembly. HRP were encapsulated within the 8 nm internal tES aqueous cavity using a simplified loading procedure. Time-course experiments demonstrated that unprotected HRP loses 95% of activity after 1 month of lyophilized storage. After encapsulation within tES nanoparticles, 70% of HRP activity was recovered, representing a 14-fold improvement and this effect was reproducible across a range of storage temperatures. To our knowledge, these results represent the first reported use of nanoparticle encapsulation to stabilize a functional macromolecule during lyophilization. Thermostable nanoencapsulation may be a useful method for the long-term storage of labile proteins.
Folate Functionalized Lipid Nanoparticles for Targeted Therapy of Methicillin-Resistant Staphylococcus aureus
Authors: Vanamala Kushal , Bhise Ketki , Sanchez Hiram , Kebriaei Razieh , Luong Duy , Sau Samaresh , Abdelhady Hosam , Rybak Michael J. , Andes David , Iyer Arun K.
Published: October 2021

Methicillin-resistant Staphylococcus aureus (MRSA), commonly called a superbug, is a highly alarming antibiotic-resistant population of Staphylococcus aureus (S. aureus) bacteria. Vancomycin (VAN) was first approved by the FDA in 1988, and it is still regarded as the treatment of choice for MRSA. The efficacy of VAN treatment has become less effective due to the development of VAN resistance in MRSA and the potential for nephrotoxicity. This study aims to improve the efficacy of VAN treatment by identifying the folate receptor for MRSA infected tissues and developing folate decorated lipid nanoparticles containing VAN (LVAN). In comparison to conventional VAN, LVAN showed a higher bactericidal effect and a superior ability to inhibit biofilm in MRSA with an enhanced accumulation in MRSA infected thigh tissues and a reduced accumulation in kidney. The results suggested that LVAN is a promising candidate to overcome the current limitations of bacterial resistance and adverse side effects in kidneys found in VAN.
The Influence of Hop Latent Viroid (HLVd) Infection on Gene Expression and Secondary Metabolite Contents in Hop (Humulus lupulus L.) Glandular Trichomes
Authors: Patzak Josef , Henychová Alena , Krofta Karel , Svoboda Petr , Malířová Ivana
Published: October 2021

Viroids are small infectious pathogens, composed of a short single-stranded circular RNA. Hop (Humulus lupulus L.) plants are hosts to four viroids from the family Pospiviroidae. Hop latent viroid (HLVd) is spread worldwide in all hop-growing regions without any visible symptoms on infected hop plants. In this study, we evaluated the influence of HLVd infection on the content and the composition of secondary metabolites in maturated hop cones, together with gene expression analyses of involved biosynthesis and regulation genes for Saaz, Sládek, Premiant and Agnus cultivars. We confirmed that the contents of alpha bitter acids were significantly reduced in the range from 8.8% to 34% by viroid infection. New, we found that viroid infection significantly reduced the contents of xanthohumol in the range from 3.9% to 23.5%. In essential oils of Saaz cultivar, the contents of monoterpenes, terpene epoxides and terpene alcohols were increased, but the contents of sesquiterpenes and terpene ketones were decreased. Secondary metabolites changes were supported by gene expression analyses, except essential oils. Last-step biosynthesis enzyme genes, namely humulone synthase 1 (HS1) and 2 (HS2) for alpha bitter acids and O-methytransferase 1 (OMT1) for xanthohumol, were down-regulated by viroid infection. We found that the expression of ribosomal protein L5 (RPL5) RPL5 and the splicing of transcription factor IIIA-7ZF were affected by viroid infection and a disbalance in proteosynthesis can influence transcriptions of biosynthesis and regulatory genes involved in of secondary metabolites biosynthesis. We suppose that RPL5/TFIIIA-7ZF regulatory cascade can be involved in HLVd replication as for other viroids of the family Pospiviroidae.
Effect of Fiber Reinforcement Type on the Performance of Large Posterior Restorations: A Review of In Vitro Studies
Authors: Mangoush Enas , Garoushi Sufyan , Lassila Lippo , Vallittu Pekka K. , Säilynoja Eija
Published: October 2021

To reinforce extensively prepared cavities, different types of fiber reinforcement are utilized. Polyethylene and glass fibers are the most commonly used fibers in that purpose; each type has its own advantages over the other type. Therefore, the aim of this study is to review the literature to evaluate and compare the influence of different fiber reinforcement types on the performance of posterior large composite restorations. Two independent authors performed a comprehensive literature search using MEDLINE/PubMed, Google Scholar, and a manual search for cross references until July 2021. Authors selected only studies that contain comparisons between glass (continuous or short) and polyethylene (woven) fiber-reinforced composites (FRCs) in posterior cavities of human teeth, and that report the effect of fiber inclusion on fracture resistance, microleakage, and marginal adaptation of restorations. A number of 2711 potentially relevant articles were obtained from the electronic search. After extensive assessment, 2696 articles were ineligible to be included in the review, and only 15 articles met the inclusion criteria. Four out of nine studies, which tested the fracture resistance of FRC restorations, revealed similar performance of the glass and polyethylene fibers. The rest of the studies (n = 5) revealed statistically significant differences between the two types of fiber reinforcement, with the majority showed superior reinforcement of glass fiber. Moreover, the reviewed studies revealed that, using fibers within the composite restorations would reduce the microleakage and improve the marginal adaptation of the restoration regardless of the fiber type. FRCs tend to strengthen the restorations of structurally compromised teeth and improve their performance compared to plain composite restorations.
Improving Ship Detection in Clutter-Edge and Multi-Target Scenarios for High-Frequency Radar
Authors: Yang Zhiqing , Zhou Hao , Tian Yingwei , Huang Weimin , Shen Wei
Published: October 2021

As one of the main sensors for continuous maritime measurements of sea state parameters, high-frequency surface wave radar (HFSWR) also plays an important role in ship detection and tracking. Compact HFSWR often suffers from missing targets, especially when the target appears near the Doppler region with heavy sea clutter or near another target in a multi-target scenario. To address this problem, an automatic ship detection method based on time–frequency (TF) analysis is presented in this paper. The TF target ridge areas are extracted in the TF image via the eigenvalues of the Hessian matrix, image edge detection, and local maximum search. Then, whether ship signals exist in the TF ridges or not is decided by a decision threshold that is calculated by fitting the probability distribution function (PDF) of sea clutter in the TF domain. The proposed TF method can separate TF ridges of similar Doppler frequency and performs constant false alarm rate (CFAR) detection for TF targets, which facilitates detecting these targets that are masked by sea clutter and other large targets. Experimental results show that the number of detected ships that match with the automatic identification system (AIS) records is four times more than that obtained by the conventional constant false alarm rate (CFAR) detectors and 1.3 times more than that by the state-of-the-art TF method in consideration of approximately the same number of detected targets.
GNSS-IR Snow Depth Retrieval from Multi-GNSS and Multi-Frequency Data
Authors: Tu Jinsheng , Wei Haohan , Zhang Rui , Yang Lei , Lv Jichao , Li Xiaoming , Nie Shihai , Li Peng , Wang Yanxia , Li Nan
Published: October 2021

Global navigation satellite system interferometric reflectometry (GNSS-IR) represents an extra method to detect snow depth for climate research and water cycle managing. However, using a single frequency of GNSS-IR for snow depth retrieval is often found to be challenging when attempting to achieve a high spatial and temporal sensitivity. To evaluate both the capability of the GNSS-IR snow depth retrieved by the multi-GNSS system and multi-frequency from signal-to-noise ratio (SNR) data, the accuracy of snow depth retrieval by different frequency signals from the multi-GNSS system is analyzed, and a joint retrieval is carried out by combining the multi-GNSS system retrieval results. The SNR data of the global positioning system (GPS), global orbit navigation satellite system (GLONASS), Galileo satellite navigation system (Galileo), and BeiDou navigation satellite system (BDS) from the P387 station of the U.S. Plate Boundary Observatory (PBO) are analyzed. A Lomb–Scargle periodogram (LSP) spectrum analysis is used to compare the difference in reflector height between the snow-free and snow surfaces in order to retrieve the snow depth, which is compared with the PBO snow depth. First, the different frequency retrieval results of the multi-GNSS system are analyzed. Then, the retrieval accuracy of the different GNSS systems is analyzed through multi-frequency mean fusion. Finally, the joint retrieval accuracy of the multi-GNSS system is analyzed through mean fusion. The experimental shows that the retrieval results of different frequencies of the multi-GNSS system have a strong correlation with the PBO snow depth, and that the accuracy is better than 10 cm. The multi-frequency mean fusion of different GNSS systems can effectively improve the retrieval accuracy, which is better than 7 cm. The joint retrieval accuracy of the multi-GNSS system is further improved, with a correlation coefficient (R) between the retrieval snow depth and the PBO snow depth of 0.99, and the accuracy is better than 3 cm. Therefore, using multi-GNSS and multi-frequency data to retrieve the snow depth has a good accuracy and feasibility.
Sensitivity Improvement of Phi-OTDR by Fiber Cable Coils
Authors: Stepanov Konstantin V. , Zhirnov Andrey A. , Koshelev Kirill I. , Chernutsky Anton O. , Khan Roman I. , Pnev Alexey B.
Published: October 2021

We present a theoretical and experimental study in which we increased the sensitivity of a phase-sensitive optical time-domain reflectometer (phi-OTDR). This was achieved by constructing coils in the sensor cable, which increased the total amplitude of the impact on the fiber. We demonstrate this theoretically using the example of a phase-sensitive reflectometer model and practically in testing grounds with a buried nearby conventional sensor and a sensor with coils. The sensitivity increased 2.2 times. We detected 95% of events when using coils, versus 20% when using a straight cable. The suggested method does not require any modifications to the device.
Detecting Proximity with Bluetooth Low Energy Beacons for Cultural Heritage
Authors: Barsocchi Paolo , Girolami Michele , La Rosa Davide
Published: October 2021

The RE.S.I.STO project targets visitors of Pisa medieval city, with the goal of providing high-quality digital contents accessible with smart devices. We describe the design, implementation and the test phases of the RE.S.I.STO application, whose goal is to automatically detect the proximity between visitors and artworks. Proximity is detected with a set of algorithms based on the analysis of Bluetooth Low Energy beacons. We detail our experimental campaigns which reproduce several museum layouts of increasing complexity at two pilot sites, and we compute the performance of the implemented algorithms to detect the nearby artworks. In particular, we test our solution in a wide open space located in our research institute and by performing a real deployment at the Camposanto Monumentale located in Pisa (Italy). The obtained performance varies in the range of 40% to perfect accuracy, according to the complexity of the considered museum layouts. We also describe a set of stress and stability tests aimed at verifying the robustness of the application during the data collection process. Our results show that the mobile application is able to reduce the beacon loss rate, with an average value of 77% of collected beacons.
Direct-to-Satellite IoT Slotted Aloha Systems with Multiple Satellites and Unequal Erasure Probabilities
Authors: Tondo Felipe Augusto , Montejo-Sánchez Samuel , Pellenz Marcelo Eduardo , Céspedes Sandra , Souza Richard Demo
Published: October 2021

Direct-to-satellite Internet of Things (IoT) solutions have attracted a lot of attention from industry and academia recently, as promising alternatives for large scale coverage of a massive number of IoT devices. In this work, we considered that a cluster of IoT devices was under the coverage of a constellation of low-Earth orbit (LEO) satellites, while slotted Aloha was used as a medium access control technique. Then, we analyzed the throughput and packet loss rate while considering potentially different erasure probabilities at each of the visible satellites within the constellation. We show that different combinations of erasure probabilities at the LEO satellites and the IoT traffic load can lead to considerable differences in the system’s performance. Next, we introduce an intelligent traffic load distribution (ITLD) strategy, which, by choosing between a non-uniform allocation and the uniform traffic load distribution, guarantees a high overall system throughput, by allocating more appropriate amounts of traffic load at different positions (i.e., different sets of erasure probabilities) of the LEO constellation with respect to the IoT cluster. Finally, the results show that ITLD, a mechanism with low implementation complexity, allows the system to be much more scalable, intelligently exploiting the potential of the different positions of the satellite constellation.
Study of a Mode Separation Due to Polarization Existing in a Cavity-Enhanced Absorption Spectroscopy
Authors: Guan Shiyu , Chen Dingbo , Cao Huilin , Tan Zhongqi
Published: October 2021

A special phenomenon of resonance mode separation is observed during the study of a high sensitivity folded-cavity enhanced absorption spectroscopy for the measurement of trace gases. The phenomenon affects the measurement of gas absorption spectrum in the cavity. This resonant mode separation phenomenon of the resonant cavity is different from the resonant modes previously observed in linear-cavity enhanced absorption spectroscopy systems. To explore the mechanism of this phenomenon, a series of hypotheses are proposed. The most likely reason among these hypotheses is based on the different reflectance properties of the plane mirror at the fold of the cavity for S-polarized light and P-polarized light. Based on the matrix calculation method, the different reflectance and phase shift of the plane mirror for S-polarized light and P-polarized light are analyzed theoretically, and the results are in better agreement with the phenomena observed in the experiment. Finally, in order to eliminate the resonant mode separation phenomenon, line polarizers were added. By improving the system, the cavity enhanced absorption spectrum of residual water vapor in the cavity was successfully measured, and a minimum detectable absorption coefficient of αmin = 7.6 × 10−9 cm−1 can be obtained in a single laser scan of 10 s.
Scoring Performance on the Y-Balance Test Using a Deep Learning Approach
Authors: Gil-Martín Manuel , Johnston William , San-Segundo Rubén , Caulfield Brian
Published: October 2021

The Y Balance Test (YBT) is a dynamic balance assessment typically used in sports medicine. This work proposes a deep learning approach to automatically score this YBT by estimating the normalized reach distance (NRD) using a wearable sensor to register inertial signals during the movement. This paper evaluates several signal processing techniques to extract relevant information to feed the deep neural network. This evaluation was performed using a state-of-the-art human activity recognition system based on recurrent neural networks (RNNs). This deep neural network includes long short-term memory (LSTM) layers to learn features from time series by modeling temporal patterns and an additional fully connected layer to estimate the NRD (normalized by the leg length). All analyses were carried out using a dataset with YBT assessments from 407 subjects, including young and middle-aged volunteers and athletes from different sports. This dataset allowed developing a global and robust solution for scoring the YBT in a wide range of applications. The experimentation setup considered a 10-fold subject-wise cross-validation using training, validation, and testing subsets. The mean absolute percentage error (MAPE) obtained was 7.88 ± 0.20%. Moreover, this work proposes specific regression systems to estimate the NRD for each direction separately, obtaining an average MAPE of 7.33 ± 0.26%. This deep learning approach was compared to a previous work using dynamic time warping and k-NN algorithms, obtaining a relative MAPE reduction of 10%.