bluuebunny/crossref_metadata_2025 huggingface dataset:
PENGARUH PENGUNGKAPAN ENVIRONMENTAL, SOCIAL, AND GOVERNANCE (ESG) TERHADAP EFISIENSI INVESTASI
Authors: Hake Sheiladhita Novrina Ruth ,   Unika Widya Mandala Surabaya, Shanti Shanti ,   Unika Widya Mandala Surabaya
Published: July 2024

Investasi merupakan tindakan menyerahkan sejumlah dana atau sumber daya untuk menghasilkan imbalan sebesar mungkin di masa depan. Perusahaan harus berinvestasi secara efektif jika ingin investasi tersebut menambah nilai dan menawarkan berbagai keuntungan. Efisiensi digambarkan sebagai proses di mana sumber daya digunakan dengan hemat untuk mencegah pemborosan sumber daya. Efisiensi investasi menunjukkan pengambilan keputusan yang baik dari perusahaan ketika mengalokasikan sumber daya. Penelitian ini bertujuan untuk menguji pengaruh pengungkaan environment, social, and governance (ESG) terhadap efisiensi investasi dari perusahaan manufaktur yang terdaftar di Bursa Efek Indonesia pada tahun 2019-2021. Penelitian ini mengguanakan variabel dependen yaitu efisiensi investasi dan variabel independen yaitu pengungkapan environmental, social, and governance (ESG). Penelitian ini menggunakan metode kuantitatif, dengan populasi penelitian yang digunakan adalah perusahaan manufaktur yang terdaftar di Bursa Efek Indonesia tahun 2019-2021. Dengan menggunakan purposive sampling dalam pemilihan sampel maka diperoleh sebanyak 153 sampel penelitian untuk tiga tahun berturut-turut (2019-2021). Teknik pengumpulan data menggunakan metode dokumentasi dengan data yang diperoleh dari situs Bursa Efek Indonesia dan app.esgbook.com sedangkan untuk teknik analisis data yang digunakan dalam penelitian ini adalah teknik analisis regresi berganda yang diolah dengan menggunakan SPSS. Temuan dari penelitian ini mengungkapkan pengungkapan ESG berpengaruh positif terhadap efisiensi investasi.
COPING WITH DEMANDING SITUATIONS IN MANAGERIAL WORK IN THE CONTEXT OF THE PERSONALITY TRAITS OF MANAGERS
Authors: GABRHEOVÁ GABRIELA , ONDRIJOVÁ IVANA , SCIRANKOVÁ ANNAMÁRIA
Published: July 2024

Effectively resolving demanding situations in managerial work and the residues of these processes can significantly impact the quality of a manager's life, both mentally and physically. The goal of research project is to focus attention on identifying and specifying selected personality traits of managers that are related to coping with demanding situations. The results confirmed the expected correlations between the ways of behaving in demanding managerial work situations and the features of interpersonal behavior of managers. The acquired knowledge also reveals risk factors that can significantly reduce the level of a manager's work and are thus an important aspect of decision-making not only in the process of selecting people for managerial positions, but also in the education and training of managers.
Effect of Maladjustment Factors on Job Performance of Career Counselor: Mediating Effect of Self-reflection, Professional Occupation, and Intrinsic Job Satisfaction
Authors: Na Young Sook , Park Hui Hyun ,   Korean Correction Counseling Psychological Association
Published: July 2024

This study was conducted to examine the effect of maladjustment factors on the job performance of counselors and to explore ways to improve them. The subjects of this study were a total of 212 career counselors working at institutions of the Employment and Welfare Plus Center of the Ministry of Employment and Labor, private consignment institutions of the Ministry of Employment and Labor, local government job centers, new work support centers, and university Job Plus centers. The survey was conducted online from April 1st to April 26th, 2023 through the in-house bulletin board or e-mail of each institution. The measurement tools used a work environment scale, a self-reflection scale, a professional occupation scale, an intrinsic satisfaction scale, and a job performance scale. Data analysis was performed using the SPSS 23.0 program and the PROCESS macrover. 3.3 program. The research results were as follows.: First, in the relationship between maladaptive factors and job performance of career counselors, a significant multi-mediating effect was found in which self-reflection and professionalism sequentially influence each other. Second, in the relationship between maladaptive factors and career counselors' job performance, a significant multimediating effect was found in which self-reflection and intrinsic job satisfaction sequentially influence each other. These results confirmed that it is important to improve job performance by improving career counselor's self-reflection, intrinsic job satisfaction, and expertise in order to reduce the influence of maladjustment factors. It was confirmed that career counselors with maladjustment factors had a great influence on the performance of career counseling in terms of self-reflection and recognition of professionalism. This suggests that there is a need to provide systematic continuing education for career counselors and opportunities to share field experiences.
Historical Forms and Principles of Democracy
Authors: Patiev N.
Published: July 2024

Examines the political view and historical forms of democracy, democratic values and the democratic regime. It is impossible to give a one-sided definition of democracy - one can observe its versatility in a full reflection of its essence. If we understand it from the point of view of political science, then it manifests itself as a form of state in all its manifestations. This tradition originates from ancient political thought. The methodological basis of the study is relativity, systematicity, scientific objectivity, historical, social and other principles.
Formation of the Idea of Cloud Technology and Its Legal Features in the Kyrgyz Republic
Authors: Semenov N. , Semenov S.
Published: July 2024

The article discusses the ideas of forming cloud technologies in the general information structure of the Kyrgyz Republic, features, disadvantages, as well as ways to further improve cloud systems. The experience of building cloud technologies at various stages of information development of the state in the form of creating strategies, programs and concepts has been studied. Proposals for the development of cloud technologies aimed at improving individual management methods in the system of state information complexes have been identified.
Once Again on the Concept of Evidence in Civil Procedure Law
Authors: Тoktakunkyzy G. , Matisakova Z.
Published: July 2024

The article discusses the process of proof and its stages, classification of evidence. The role of forensic evidence in resolving disputes in the process is becoming increasingly important. Problems of collecting and interpreting evidence associated with the evidentiary process are of particular importance in legal disputes, since they are means of proof that determine the outcome of the judicial consideration of the dispute. The purpose of the evidence is to establish factual circumstances relevant to the resolution of the civil case. When studying scientific work, the goal is to analyze the place of judicial evidence in civil proceedings and the problems in it using the example of domestic and foreign scientific literature. The methodological basis of the study is relativity, consistency, and formal legal methods. The results obtained in the scientific work are recommended for use in judicial practice, in the science of civil procedural law, in the science of the law of evidence.
Linguistic and Didactic Foundations of Teaching a Non-native Language in Pedagogical Universities of the Kyrgyz Republic
Authors: Segizbaeva N.
Published: July 2024

This article discusses the linguodidactic foundations of teaching a non-native language in universities of the Kyrgyz Republic. The main purpose of the article is to show the theoretical and methodological apparatus that is aimed at learning and teaching a language as a second language. Lingvodidactics is an important area of ​​research in the methodology of teaching non-native languages ​​in pedagogical universities. The main goal of linguodidactics is the development of scientifically based teaching methods and techniques that allow students-future teachers to effectively develop communicative competence in the target language. The key principle of linguodidactics is taking into account the characteristics of students’ native language and interlingual interference when mastering a non-native language. This allows you to identify and prevent typical mistakes of students, as well as optimize the process of mastering language and speech material.
Study of the Effectiveness of Personalized Learning in the Context of the Intellectual Development of Students in the Classroom
Authors: Feng Yuan
Published: July 2024

The research area of this article is the analysis of the form and structure of personalized learning as a research strategy, the traditional approach to the model of intellectual education as a practical direction. As a goal, teach the teacher in the classroom intellectual education. Intellectual education is an educational complex that combines “education based on artificial intelligence and traditional education”, and is also the main direction of the transformation of public education into a digital large-scale education model. In the field of education, using artificial intelligence, big data, blockchain and other new digital technologies, smart education classroom is gradually updating the process of education implementation, turning it into a transformed form of data classroom and personalized classroom, and in the era of big data and information education - into smart education, integrating multimedia information technology into the educational process. It will be. Teaching and assimilation of the subject. The concept of online classrooms has been developed. Finally, according to the direction to think about the development of modern education technology, this article will analyze the results of the introduction of intelligent education technology. Personalized learning as well as the introduction of personalized guidance and support for students, create intelligent classrooms, provide students with a better understanding of the learning environment.
ASPEK COMPETENCY CIRCLE-P SELEBGRAM DALAM MENGKONTRUKSI PERSONAL BRANDING UNTUK MENINGKATKAN KETERLIBATAN DARING AUDIENCE PADA ENDORSEMENT
Authors: Sa'adah Siti , Mutiaz Intan Rizky
Published: July 2024

Abstrak
Instagram sebagai media sosial yang memiliki ekosistem tersendiri mampu menjadi medium bagi para selebgram untuk mengkontruksi dirinya sekaligus memonetisasinya melalui kesepakatan dukungan komersial. Penelitian ini mengulas konsep personal branding yang dikontruksi selebgram, konten endorsement produk fesyen, dan keterlibatan daring audience untuk mengidentifikasi hubungan antara personal branding selebgram dan konten endorsement selebriti instagram dalam menciptakan dan meningkatkan keterlibatan daring audience. Penelitian ini bertujuan untuk mengkaji personal branding selebgram melalui konten endorsement di Instagram yang mempengaruhi keterlibatan daring audience dengan menggunakan pendekatan kualitatif yang didasarkan pada observasi, dokumentasi, dan wawancara kepada audience. Metode penelitian yang dilakukan adalah studi kasus dan reception studies dengan pendekatan deskriptif komparatif dengan melakukan observasi, dokumentasi imej, dan wawancara.  Hasil penelitian yaitu; selebgram mengkontruksi diri dengan cara membangun personal branding Circle-P aspek competency pada profil diri secara keseluruhan, yaitu meliputi: beda autentik (internal dan eksternal), menjadi diri sendiri (ketekukan melatih diri, kegigihan menemukan keberuntungan), asosiasi reputasi, personality (VAK, pikiran, tindakan, kontrol), dan penampilan diri (Outfit dan gesture).
 
Kata Kunci: konten endorsement, keterlibatan daring audience, personal branding, selebgram
 
Abstract
Instagram as a social media that has its own ecosystem can be a medium for celebrities to construct themselves as well as monetize it through commercial support deals. This study reviews the concept of personal branding constructed by celebgrams, fashion product endorsement content, and Online audience engagement to identify the relationship between personal branding celebgrams and Instagram celebrity endorsement content in creating and increasing Online audience engagement. This study aims to examine the personal branding of celebgrams through endorsement content on Instagram that affects the Online engagement of the audience using a qualitative approach based on observation, documentation, and interviews to the audience. The research methods carried out are case studies and reception studies with a comparative descriptive approach by making observations, image documentation, and interviews.  The findings obtained are; Celebgrams construct themselves by building personal branding Circle-P aspects of competency on the overall self-profile, which include: authentic differences (internal and external), being yourself (bending self, persistence in finding luck), reputation associations, personality (VAK, thoughts, actions, controls), and self-appearance (outfits and gestures).
 
Keywords: celebgram, endorsement content, online audience engagement, personal branding
PERANCANGAN KARTU PERMAINAN “MITKIT” TENTANG MITIGASI BENCANA ALAM UNTUK ANAK USIA 9-12 TAHUN
Authors: Streit Aprilia Kartini , Seputro Vania Nasya
Published: July 2024

Abstrak
Indonesia sebagai negara dengan letak geografis yang unik membuat potensi dan intensitas bencana alamnya tergolong tinggi. Anak-anak memiliki kerentanan bencana yang lebih tinggi dibandingkan dengan orang dewasa. Maka dari itu, dirancanglah kartu permainan MitKit sebagai media pengenalan dan edukasi tentang bencana alam serta cara mitigasinya untuk anak usia 9-12 tahun. Jenis penelitian yang digunakan adalah kualitatif-deskriptif, didukung dengan kuisioner dalam pengumpulan data. Adapun metode pengumpulan data yaitu studi kepustakaan dan observasi. Metode perancangan yang digunakan adalah Research and Development dengan pendekatan model ADDIE yang terdiri dari 5 tahap, yaitu: Analysis, Design, Development, Implementation dan Evaluation. Hasil penelitian berupa kartu permainan tentang mitigasi bencana alam, dilengkapi dengan maskot kucing, sebagai salah satu hewan paling populer di dunia, untuk mengedukasi anak-anak tentang bencana alam dan serangkaian upaya yang dapat dilakukan untuk mengurangi risiko bencana alam. Berdasarkan penelitian dan perancangan ini, penulis berharap MitKit dapat menjadi media belajar yang interaktif dan menyenangkan bagi anak, serta menjadi solusi bagi orang tua dalam memberikan penjelasan tentang bencana alam kepada anaknya. Dengan mengetahui dan mengingat berbagai cara mitigasi bencana alam, diharapkan anak dapat tetap tenang di saat darurat dan mampu meminimalisir dampak bencana alam.
 
Kata Kunci: kartu permainan, bencana alam, mitigasi, edukasi
 
Abstract
Indonesia as a country with a unique geographical location makes the potential and intensity of natural disasters relatively high. Children have a higher vulnerability to disasters than adults. Therefore, the MitKit card game was designed as a medium for introduction and education about natural disasters and how to mitigate them for 9-12 years old children. The type of research used is qualitative-descriptive, supported by questionnaires in data collection. The data collection methods are literature study and observation. The design method used is Research and Development with the ADDIE model approach which consists of 5 stages: Analysis, Design, Development, Implementation and Evaluation. The results of the research are a playing cards about natural disaster and ways to reduce the risk of natural disasters, designed with a cat mascot, which is one of the most popular animals in the world. From this research and design, the author hopes that MitKit can become an interactive and fun learning media for children, also a solution for parents in providing explanations about natural disasters to their children. By knowing and remembering various ways of mitigating natural disasters, it is hoped that children can remain calm in times of emergency and be able to minimize the impact of natural disasters.
 
Keywords: card game, natural disasters, mitigation, education
Urgensi kemampuan analogi siswa terhadap pembelajaran matematika
Authors: Damayanti Desi , Maryati Iyam , Sundayana Rostina
Published: July 2024

Kemampuan analogi sangat penting bagi siswa dalam pembelajaran matematika, dengan kemampuan analogi, siswa dapat meningkatkan daya ingat dalam hal konsep matematika untuk jangka panjang, namun pada kenyataannya Kemampuan analogi matematis siswa tergolong masih rendah. Hal tersebut dikarenakan masih belum terbiasanya siswa berlatih menggunakan analogi dalam bermatematika. Tujuan penelitian ini adalah untuk mengkaji urgensi kemampuan analogi terhadap pembelajaran matematika. Metode yang digunakan dalam penelitian ini adalah kajian pustaka (literatur review). Peneliti memilih 32 artikel dari Google Scholar, ResearchGate, DOAJ dan SINTA, serta mempunyai keterkaitan dengan kemampuan analogi matematis kemudian diidentifikasi sesuai dengan kesamaan karakteristiknya. Hasil penelitian ini menyatakan bahwa kemampuan analogi matematis siswa mempunyai urgensi yang tinggi dalam pembelajaran matematika.
FACTORS ASSOCIATED WITH THE ULTRASOUND EXAMINATION SELECTION BY PREGNANT WOMEN IN LEREP HEALTH CENTER
Authors: Alfiani Novita , Suraningsih Nanik
Published: July 2024

ABSTRACT
Ultrasound is a way to find out the fetus’s condition in the body during prenatal care (antenatal care). This research aim was to determine the correlation between knowledge, parity, motivation, and husband's support with the choice of ultrasound examination by pregnant women at the Lerep Primary Health Care, Semarang Regency. This research is a descriptive study which is an analytic survey with a cross-sectional design. The population used in this research were third-trimester pregnant women who had their pregnancies checked at the Lerep Primary Health Care, Semarang Regency, with as many as 98 people. Sampling was carried out using non-probability sampling with accidental sampling techniques and a total of 50 people were obtained. Data collection was carried out using a questionnaire. Data analysis using chi-square.
The results showed that there was a significant relationship between the knowledge of pregnant women and the choice of ultrasound examination, there was no significant relationship between parity and the choice of ultrasound examination, and there was no significant relationship between the husband's support with the choice of ultrasound examination. With this research, it is hoped that primary health care can give some improvement in education and information for every pregnant woman who visits prenatal care about the choice of doing an ultrasound examination so that pregnant women understand the benefits of ultrasound examination.

Keywords: pregnant woman, Pregnancy test, USG
Investigating the Efficacy of Dry Region Zinc Solubilizing Bacteria for Growth Promotion of Maize and Wheat under Axenic Conditions
Authors: Asghar Muhammad Umair , Hussain Azhar , Anwar Hammad , Dar Abubakar , Ahmad Hafiz Tanvir , Nazir Qudsia , Tariq Nadeem , Jamshaid Muhammad Usman
Published: July 2024

Zinc (Zn) plays a crucial role as a micronutrient, essential for the growth, development, and proper functioning of plants. Pakistani soils are deficient of micronutrient especially zinc. Supplementation of Zn fertilizers cannot do the job because only a small fraction of Zn is available to plants and rest is fixed in soil. To enhance the Zn availability different methods are being used which are expensive. Zinc solubilizing bacteria (ZSB) can be a potential candidate for solubilization of Zn and growth promoting activities in cereals. The present study aimed to evaluate the potential of dry region bacterial strains of Bahawalpur for improving Zn uptake and growth improvement in wheat and maize. Rhizosphere samples of wheat and maize were taken from the dry regions of Bahawalpur and bacterial strains were isolated and tested for their Zn solubilizing ability and screened for urease, siderophore activity, exopolysaccharide production, phosphorus solubilization and plant growth promoting abilities. Under axenic conditions selected isolates were further screened to assessed for improving the growth of wheat and maize seedlings. The rhizobacterial isolates IUB-34, IUB-80, IUB-93 and IUB-96 performed best to improve growth, physiology, biochemical attributes and root colonization. The maximum increase in root and shoot lengths were recorded 32.2 and 35.7% in wheat under IUB-34 application and in maize 43.8 and 39.9%, respectively, under IUB-96 application as compared to uninoculated control. Furthermore, maximum root colonization was also recorded under IUB-34 in wheat and IUB-96 in maize seedlings. Moreover, the biochemical characterization of selected isolates showed IAA (Auxins) production, protease, catalase, cellulose degradation, HCN and ACC-deaminase activities by these strains. However, only IUB-34 and IUB-96 were found positive for oxidase activity. Therefore, study concluded that use of dry region ZSB can solubilize Zn and make it available for plant to improve growth of wheat and maize. So, these isolates may be utilized for coating urea as a biofertilizer to increase the biofortification in cereals.
Return Intentions of Romanian Migrants From the United Kingdom: An Exploratory Approach
Authors: Matei Aniela , Ghinăraru Cătălin , Ghența Mihaela , Mladen-Macovei Louise
Published: July 2024

Return migration is a complex phenomenon driven by a combination of individual, familial, societal, and economic factors. This study aims to discover the key reasons that limit return intentions of Romanian migrants from United Kingdom to their country of origin. Using a mix of qualitative and quantitative research methods, semi-structured interviews with experts involved in the integration of Romanians in the United Kingdom, and an online survey among Romanian migrants, this study identifies a series of factors that determine Romanian workers’ return intentions. The results indicate that available jobs in the communities of origin in Romania where they have families, and where family solidarity networks are created, are important drivers of return migration. The need to improve government measures to stimulate the return of Romanian workers must be seen in the broader context of the functionality of families with migrant workers, considering both the positive and negative consequences of labour migration for the quality of family life, and the care provision for dependents (children and elderly people).
Availability, distribution and quality of agro-industrial byproducts and compound feeds in Ethiopia
Authors: Feyisa Tesfaye , Tolera Adugna , Nurfeta Ajebu , Balehegn Mulubrhan , Adesogan Adegbola
Published: July 2024

Livestock production is a major contributor to the national economy in Ethiopia, of which ruminants, particularly cattle, are the dominant and most important livestock species. Limited supply of quality feeds is a major constraint to the improvement of livestock production in Ethiopia. The expansion of agro-industries in Ethiopia, over the last three decades, has created an opportunity for an increased supply of agro-industrial byproducts as a source of feed for livestock. This study was conducted to assess the availability, distribution, and quality of agro-industrial byproducts and compound feeds in Ethiopia. About 310 flour mill factories, 194 oil factories, 13 brewery factories, 7 sugar factories, 4 malt factories, 2 meat and bone meal processing plants, 8 limestone factories, and 112 feed processing plants were surveyed to assess the availability and distributions of agro-industrial byproducts and compound feeds. A total of 757 feed samples were collected for evaluating nutritional values. The annual production of agro-industrial byproducts ranges from 18,065 tons DM/year (abattoir byproducts) to 3,092,035 tons DM/year (malt byproducts) and the total annual production of the different agro-industrial by-products amounts to 5,245,854 tons DM. The annual production of compound feeds was reported to be 5,812,608 tons DM. All agro-industries are processing under their capacity, ranging from 11.7% in sugar factories to 93% in breweries. Shortage and seasonal fluctuation of supply of raw materials (34.8%), high price and price fluctuations of raw materials (17.5%) and electric power interruptions (17.5%) were the major challenges faced by different agro-industries. The crude protein (CP) concentrations of agro-industrial byproducts ranges from 6.2% in maize grain screening to 15.9% in wheat bran for flour mill byproducts; 28% in cotton seed cake to 49.7% in groundnut cake for oilseed cakes and 14.8% in areqe atela to 24.8% in brewery spent grains for brewery and local distillery byproducts. High protein or energy contents of agro-industrial byproducts indicated their potential to be utilized in intensive livestock rations. Thus, it is necessary to create enabling conditions to allow the existing agro-industries to operate at full capacity and to attract new ones into the business to boost the production and availability of agro-industrial byproducts needed as main inputs to manufacture compound feeds.
Genomic characterisation of generic Escherichia coli from food-producing animals and products of animal origin in South Africa
Authors: Malesa Refiloe , Pierneef Rian , Magwedere Kudakwashe , Mafuna Thendo , Matle Itumeleng
Published: July 2024

Escherichia coli is found in diverse environmental niches, including meat and meat products, and is known for its significance in both food safety and public health. In South Africa, whole genomic sequencing (WGS) efforts for E. coli are overwhelmingly skewed toward human isolates with limited studies conducted on non-human isolates. Therefore, the aim of this study was to use WGS to characterise generic strains of E. coli isolated from animal specimens, meat, and meat-based products in South Africa. Based on WGS analysis, a total of 35 E. coli strains were grouped into five phylogroups (A, B1, B2, C, and E), with A (46%) being the most predominant. Virulence-associated genes identified the isolates as either extra-intestinal pathogenic E. coli (69%) or intestinal pathogenic E. coli (31%) pathotypes. Twenty-three different serotypes were identified, with O101:H37 (17%), O2:H4 (17%), O6:H5 (13%), and O64:H19 (9%) being the predominant ones. Among the 19 different sequence types (STs), ST1858, ST975, and ST10 were the most prevalent (11% each). Various virulence genes, antimicrobial resistance genes, and genetic mobile elements carrying Tn2, IS26, and Tn6196 elements were detected, with the disinfectant resistance sitABCD being the most predominant. The type 1 CRISPR system which functions by storing records of previous invasions to provide immunological memory for a rapid and robust response upon subsequent viral infections was detected in all isolates, consisting of subtypes I-E (86%), I-A (57%), and I-F (11%). The findings of this study provide an insight into the genetic diversity of generic E. coli isolates from animal species, meat, and meat-based products in South Africa.
Synthesis, characterization, and anti-cancer potential of novel p53-mediated Mdm2 and Pirh2 modulators: an integrated In silico and In vitro approach
Authors: Niazi Sarfaraj , Kavana C. P. , Aishwarya H. K. , Dharmashekar Chandan , Jain Anisha , Wani Tanveer A. , Shivamallu Chandan , Purohit Madhusudan N. , Kollur Shiva Prasad
Published: July 2024

Introduction: Leukemia is a global health concern that requires alternative treatments due to the limitations of the FDA-approved drugs. Our focus is on p53, a crucial tumor suppressor that regulates cell division. It appears possible to stabilize p53 without causing damage to DNA by investigating dual-acting inhibitors that target both ligases. The paper aims to identify small molecule modulators of Mdm2 and Pirh2 by using 3D structural models of p53 residues and to further carry out the synthesis and evaluation of hit candidates for anti-cancer potency by in vitro and in silico studies.

Methods: We synthesized structural analogues of MMs02943764 and MMs03738126 using a 4,5-(substituted) 1,2,4-triazole-3-thiols with 2-chloro N-phenylacetamide in acetone with derivatives of PAA and PCA were followed. Cytotoxicity assays, including MTT, Trypan Blue Exclusion, and MTS assays, were performed on cancer cell lines. Anti-proliferation activity was evaluated using K562 cells. Cell cycle analysis and protein expression studies of p53, Mdm2, and Pirh2 were conducted using flow cytometry.

Results: As for results obtained from our previous studies MMs02943764, and MMs03738126 were selected among the best-fit hit molecules whose structural analogues were further subjected to molecular docking and dynamic simulation. Synthesized compounds exhibited potent anti-proliferative effects, with PAC showing significant cytotoxicity against leukemia cells. PAC induced cell cycle arrest and modulated p53, Mdm2, and Pirh2 protein expressions in K562 cells. Molecular docking revealed strong binding affinity of PAC to p53 protein, further confirmed by molecular dynamics simulation.

Discussion: The study presents novel anticancer compounds targeting the p53 ubiquitination pathway, exemplified by PAC. Future perspectives involve further optimization and preclinical studies to validate PAC’s potential as an effective anticancer therapy.
Do COVID-19 related primary emotions affect risk perceptions, efficacy beliefs, and information seeking and behavior? Examining emotions as audience segments
Authors: Turner Monique M. , Lim Jong In , Jang Youjin , Heo Ruth Jin-Hee , Ye Qijia , Kim Miyeon , Lapinski Maria Knight , Peng Tai-Quan
Published: July 2024

Introduction

Primary emotions among the populace during global health crises, such as the COVID-19 pandemic, provide valuable insights. Guided by appraisal theories of emotions, this study explores emotional segmentation by mapping primary emotions related to COVID-19 and investigating their impact on cognitive and behavioral outcomes, including risk perceptions, efficacy beliefs, behavioral intentions, prevention behaviors, and information seeking/avoidance.

Methods

Study 1 surveyed young adults (N = 1,368) to investigate their emotions about COVID-19 and examine the effect of these emotions on risk perceptions, efficacy beliefs, and behavioral intentions regarding mask-wearing and physical distancing. Study 2 replicated Study 1 with a quota-based national sample of U.S. adults (N = 8,454) and further tested the effect of primary emotions on preventive behaviors and information seeking/avoidance.

Results

Results indicated that most people experienced negative emotions, such as sadness, anger, and anxiety. These emotions were associated with varying levels of risk perceptions, efficacy beliefs, behavioral intentions, actual behaviors, and information seeking/avoidance. Notably, across both studies, anxious individuals demonstrated more favorable health-related perceptions and behavioral outcomes compared to those experiencing anger.

Discussion

These findings offer insights into the emotional experiences of individuals during the COVID-19 pandemic and highlight the significant impact of these emotions on risk perceptions and health-related behaviors. Understanding these emotional responses can inform public health strategies and communication efforts during health crises.
Preliminary results on novel adjuvant combinations suggest enhanced immunogenicity of whole inactivated pandemic influenza vaccines
Authors: Peletta Allegra , Marmy Aurélie , Guzelj Samo , Ramos Barros Alcidia , Jakopin Žiga , Borchard Gerrit
Published: July 2024

Due to the inherent risk of a further pandemic influenza outbreak, there is a need and growing interest in investigating combinations of prophylactic vaccines and novel adjuvants, particularly to achieve antigen dose sparing and improved immunogenicity. Influenza is a highly variable virus, where the specific vaccine target is constantly changing, representing a major challenge to influenza vaccine development. Currently, commercial inactivated influenza vaccines have a poor CD8+ T response, which impacts cross-reactivity and the duration of response. Adjuvanted influenza vaccines can increase immune responses, thereby achieving better protection and cross-reactivity to help contain the spread of the disease. An early exploration of a hybrid cholesterol-PLGA nanoparticle delivery system containing the saponin tomatine and a NOD2 (nucleotide-binding oligomerization domain 2) agonist called SG101 was conducted. This combination was preliminarily evaluated for its ability to induce cellular immunity when combined with whole inactivated virus (WIV) influenza vaccine. After the adjuvants were manufactured using a single emulsion process, two formulations with different drug loadings were selected and physico-chemically characterized, showing sizes between 224 ± 32 and 309 ± 45 nm and different morphologies. After ensuring the lack of in vitro toxicity and hemolytic activity, a pilot in vivo assay evaluated the hybrid nanoparticle formulation for its ability to induce humoral and cellular immunity when combined with whole inactivated virus (WIV) H5N1 influenza vaccine by intramuscular administration in mice. Hemagglutinin inhibition (HAI) titers for adjuvanted groups showed no significant difference compared to the group vaccinated with the antigen alone. It was similar for CD4+ and CD8+ T cell responses, although the high drug loading formulation induced higher titers of IFNγ-positive CD8+ T cells. These proof-of-concept results encourage further investigations to develop the hybrid formulation with increased or different loading ratios, to investigate manufacturing optimization, and to evaluate the role of the individual immunostimulatory compounds in immune responses.
Identification of dominant tree species based on Resource-1 02D hyperspectral image data
Authors: Zhou Jingchun , Feng Zhanyong , Li Yiping , Wang Jinliang , Meng Xiangrui , Liu Yuan , Qiu Shaobo
Published: July 2024

Fine-grained classification of tree species by using high-spectral image data has garnered considerable attention from scholars. In this study, through field measurements from Maguan County, Wenshan Prefecture, Yunnan Province, China, high-spectral image data from the Chinese Resource-1 02D satellite were used as the data source. Various analyses were conducted on the original image’s spectral curve, the spectral curve after envelope removal, the spectral curve after first-order differential transformation, and the spectral curve after second-order differential transformation. A spectral angle mapping classification method was employed to classify and identify four dominant tree species in Maguan County, and the accuracy of the classification results was validated using a confusion matrix. Results indicate that the highest accuracy in tree species classification was achieved when first-order differential transformation and envelope removal were used for the spectral curve; the overall accuracy exceeded 95%, and the kappa value was approximately 0.95. The classification results for the spectral curve after second-order differential transformation were the lowest, with an overall accuracy of 81.69% and a kappa value of 0.76. This research demonstrates that applying first-order differential transformation or envelope removal in combination with spectral angle mapping classification considerably reduces data processing time and improves tree species classification accuracy.
DiffSim: a user-friendly tool for precise magmatic timescale determination and error propagation via major element diffusion chronometry in magmatic phases
Authors: Morgado Eduardo , Morgan Daniel J.
Published: July 2024

Diffusion chronometry is a technique gaining interest in the scientific community related to volcanology and petrology; however, modelling can be challenging for non-expert users. Here, we present DiffSim, a user-friendly standalone freeware that allows users to calculate magmatic timescales simulating 1D diffusion of major elements in olivine, orthopyroxene, titanomagnetite, and melt (inclusions). The freeware works solving the Fick’s second law equation (for both Cartesian and spherical polar coordinates, depending on the phase) using finite differences through the Crank-Nicolson method. Users must specify the initial composition vs. distance profile, the time resolution, and the intensive conditions (such as temperature, pressure, and oxygen fugacity). For orthorhombic phases, such as olivine and orthopyroxene, users must also specify the plunge and the trend of the (001)-axis and the angle traverse of the 2D section being studied. The best-fitting profile, comparing the natural (measured) and the modelled (calculated) profiles, is obtained using the least-squares fitting method in accordance with the total time specified by the user for performing the diffusion modelling. To determine the uncertainties of the timescale calculation, DiffSim propagates errors based on the uncertainties associated with each intensive condition and the experimental diffusivity measurements. DiffSim is available as executable freeware, allowing researchers and students to use diffusion chronometry to elucidate information about crustal processes with ease and precision.
SAGA: results of a second trial testing a mentalizing-based reading intervention on children and staff in early childhood education
Authors: Linnavalli Tanja , Martikainen Silja , Belfrage Filippa , Kalland Mirjam
Published: July 2024

Social–emotional development is a key factor in child well-being and development, and studying how it can be supported in early childhood is crucial. This study acted as a second trial testing the efficacy of a shared story book reading intervention combined with mentalizing discussions (SAGA), on children’s (N = 196) social–emotional development. In contrast to the first trial, the current trial utilized a group comprised of mostly multilingual children, attending daycare in a minority language. In addition, we investigated the effect of the intervention on the mentalizing capacity of the staff. The staff of the early childhood education and care (ECEC) centers were trained to lead discussions about story characters’ mental states with children three times a week. The staff’s mentalization ability was measured by the self-reported Mentalization Scale (MentS). Children’s social–emotional development was evaluated via the teacher-reported Strengths and Difficulties Questionnaire SDQ, and verbal fluency via a subtest from the NEPSY II neuropsychological test battery. After 12 weeks, the children in the SAGA group showed improvement in prosocial behavior, whereas no change was observed in the control group. Furthermore, within the SAGA group, the children showing initially lower scores for prosocial behavior displayed larger improvement compared to their peers with higher scores at baseline. No such intervention-based improvement emerged in verbal fluency. Unlike in the first trial, the intervention did not have an impact on children’s internalizing or externalizing problems. The results suggest that story reading sessions combined with mentalizing discussions about emotions, thoughts, and intentions of the story characters may support children’s social–emotional development within the realm of prosocial behavior, although the possibility to decrease children’s internalizing and externalizing problems with these sessions remains unclear based on the two trials. In addition, training the ECEC staff in mentalization theory and guiding them toward mind-related dialogs improved staff motivation to mentalize, as well as their child-related mentalization capacity.
Developing a self-determination motivation scale for English learners with Chinese background
Authors: Chiang Juiching
Published: July 2024

“Interest” is one of the most important factors that trigger English learning behavior, and self-determination theory believes that “interest” and “value” are important factors associated with target behavior. Therefore, the purpose of this study is to construct and establish a “Self-Determination Motivation Scale of English Learners” based on the core concept of self-determination theory, which encompasses the three innate needs. There were 169 participants, all university students from various majors with Chinese backgrounds, who were taking English classes at a university. The research tool is extracted from the Learning Climate Questionnaire (LCQ) (six questions on autonomy), perceived competence for learning (PCS) (four questions on competence), and basic psychological needs (BPN) in general (eight questions on relatedness) and developed an 18-item questionnaire. The data were obtained from the existing three different self-determination questionnaires and analyzed using confirmatory factor analysis (CFA). The results show that after CFA, 15 items of the scale, which were extracted from the existing three different self-made questionnaires, were maintained, and the removed three items are all reverse items. The reliability of the developed 15-item scale is 0.95. The conclusion is that the developed 15-item scale is more suitable for university English learners with a Chinese background, although the existing three different self-determination questionnaires are still valid and reliable. Three suggestions for future research include adding expert validity for item review, adjusting the number of reverse items, and having more participants to master the scale. The implications, limitations, and future research plans are presented in this paper.
Association between triglyceride to high-density lipoprotein cholesterol ratio and nonalcoholic fatty liver disease and liver fibrosis in American adults: an observational study from the National Health and Nutrition Examination Survey 2017–2020
Authors: Wang Jianjun , Li Han , Wang Xiaoyi , Shi Ruizi , Hu Junchao , Zeng Xintao , Luo Hua , Yang Pei , Luo Huiwen , Cao Yuan , Cai Xianfu , Chen Sirui , Wang Decai
Published: July 2024

Objective

This study investigated the link between triglyceride to high-density lipoprotein cholesterol (TG/HDL-C) ratio and nonalcoholic fatty liver disease (NAFLD) and liver fibrosis in American adults.

Methods

Information for 6495 participants from the National Health and Nutrition Examination Survey (NHANES) 2017–2020.03 was used for this cross-sectional study. The link between TG/HDL-C ratios and NAFLD and liver fibrosis was assessed by multiple linear regression before evaluating nonlinear correlations based on smoothed curve fitting models. Stratification analysis was then applied to confirm whether the dependent and independent variables displayed a stable association across populations.

Results

TG/HDL-C ratios were positively correlated with NAFLD, with higher ratios being linked to increased prevalence of NAFLD. After adjusting for potential confounders, the odds ratios (OR) for NAFLD patients in the fourth TG/HDL-C quartile were 3.61 (95% confidence interval [CI], 2.94–4.38) (P for trend < 0.001) in comparison with those in the first quartile after adjusting for clinical variables. However, no statistical significance was noted for the ratio for liver fibrosis after adjusting for potential confounders (P for trend = 0.07). A nonlinear correlation between TG/HDL-C ratios and NAFLD was observed based on smoothed curve fitting models. However, a nonlinear relationship between the ratios and liver fibrosis was not established. In subgroup analyses, there was an interaction between smoking status and TG/HDL-C ratio in relation to the prevalence of liver fibrosis (P for interaction < 0.001).

Conclusions

Among American adults, the TG/HDL-C ratio was noted to be nonlinearly positively associated with the prevalence of NAFLD; however, this relationship was not present in liver fibrosis.
Postpartum glucose intolerance after gestational diabetes mellitus: tailored prediction according to data-driven clusters and BMI-categories
Authors: Lesniara-Stachon Anna , Cosson Emmanuel , Lacroix Alain , Schenk Sybille , Quansah Dan Yedu , Puder Jardena J.
Published: July 2024

Objectives

To account for the heterogeneity of gestational diabetes (GDM), this study investigated tailored predictors during pregnancy and at 6-8 weeks postpartum of glucose intolerance (GI) at 1-year postpartum. We identified predictors according to data-driven clusters, analogous to the newly proposed diabetes classification, and for clinical ease also based on BMI-categories.

Methods

This is a secondary analysis of the MySweetheart trial. It included 179 women with GDM who underwent a 75g oral glucose tolerance test and HbA1c measurement at 1-year postpartum. Predictors were determined according to: a) cluster analysis based on age, BMI, HOMA-IR and HOMA-B; and b) BMI-categories (normal weight [NW], and overweight/obesity [OW/OB]).

Results

We identified two clusters during pregnancy and at 6-8 weeks postpartum (for both time points an “insulin-resistant”, and an “insulin-deficient” cluster). The “insulin-resistant” cluster was associated with a 2.9-fold (CI: 1.46-5.87; pregnancy) and 3.5-fold (CI: 1.63-7.52; at 6-8 weeks postpartum) increased risk of GI at 1-year postpartum. During pregnancy, the most relevant predictors of GI were history of previous GDM and fasting glucose for the “insulin-deficient” and NW category and HOMA-IR for the “insulin-resistant” and OW/OB category (all p ≤0.035). In the postpartum, predictors were more heterogenous and included the insulin-sensitivity-adjusted-secretion index and 1-h glucose in the “insulin-deficient” and NW women.

Main conclusions

In women with GDM, we identified “insulin-resistant” and “insulin-deficient” clusters with distinct risks of future GI. Predictors varied according to clusters or BMI-categories emphasizing the need for tailored risk assessments.
Identification of the CDH18 gene associated with age-related macular degeneration using weighted gene co-expression network analysis
Authors: Liu Guina , Tan Mingqi , Liu Rui , Lu Xuejin , Jiang Xiaoshuang , Bai Yunpeng , Guo Zhigang , Lu Fang
Published: July 2024

Purpose: Age-related macular degeneration (AMD) is a chronic and progressive macular degenerative disease that culminates in a gradual deterioration of central vision. Despite its prevalence, the key biomarkers for AMD have not yet been fully elucidated. In this study, we aimed to efficiently identify biomarkers crucial for diagnosing AMD.

Methods: Three datasets pertaining to retinal pigment epithelium (RPE)/choroid tissues associated with AMD were selected from the GEO database. The GSE50195 dataset was utilized to conduct weighted gene co-expression network analysis (WGCNA) for identifying module genes linked to AMD. KEGG and GO enrichment analyses were subsequently conducted on these module genes. GSE29801 and GSE135092 datasets were subjected to differential expression analysis to pinpoint the DEGs intersecting with the module genes. Subsequently, wet AMD (wAMD) and dry AMD (dAMD) mouse models were developed, from which RPE/choroid tissues were harvested to validate the hub genes via RT-qPCR and Western blot.

Results: Using the WGCNA, we selected the “antiquewhite4” module (r = 0.91 and p = 7e-07), which contains a total of 325 genes. Through the intersection of module genes with DEGs, nine hub genes were identified. Pathways involved in complement and coagulation cascades, ECM–receptor interactions, unsaturated fatty acid biosynthesis, and fatty acid elongation play important roles in AMD. Notably, CDH18 demonstrated notable variance across all three datasets. Post validation using RT-qPCR experiments revealed a significant downregulation of CDH18 in both dAMD and wAMD. EGLN3 was expressed at low levels in wAMD. In dAMD, EYA2, LTB, and PODXL were significantly downregulated, whereas APOC1 was notably upregulated. Western blot confirmed that CDH18 was lowly expressed in dAMD and wAMD mouse models.

Conclusion: CDH18 was identified as the key gene involved in the pathogenesis of AMD. An imbalance of the complement and coagulation cascades is a potential mechanism of AMD. This study provides a novel idea for diagnosing and treating AMD in the future.