bluuebunny/crossref_metadata_2025 huggingface dataset:
Plant Kinesin-12: Localization Heterogeneity and Functional Implications
Authors: Müller Sabine , Livanos Pantelis
Published: August 2019

Kinesin-12 family members are characterized by an N-terminal motor domain and the extensive presence of coiled-coil domains. Animal orthologs display microtubule plus-end directed motility, bundling of parallel and antiparallel microtubules, plus-end stabilization, and they play a crucial role in spindle assembly. In plants, kinesin-12 members mediate a number of developmental processes including male gametophyte, embryo, seedling, and seed development. At the cellular level, they participate in critical events during cell division. Several kinesin-12 members localize to the phragmoplast midzone, interact with isoforms of the conserved microtubule cross-linker MICROTUBULE-ASSOCIATED PROTEIN 65 (MAP65) family, and are required for phragmoplast stability and expansion, as well as for proper cell plate development. Throughout cell division, a subset of kinesin-12 reside, in addition or exclusively, at the cortical division zone and mediate the accurate guidance of the phragmoplast. This review aims to summarize the current knowledge on kinesin-12 in plants and shed some light onto the heterogeneous localization and domain architecture, which potentially conceals functional diversification.
Effects of Grape Seed Extract and Proanthocyanidin B2 on In Vitro Proliferation, Viability, Steroidogenesis, Oxidative Stress, and Cell Signaling in Human Granulosa Cells
Authors: Barbe Alix , Ramé Christelle , Mellouk Namya , Estienne Anthony , Bongrani Alice , Brossaud Adeline , Riva Antonella , Guérif Fabrice , Froment Pascal , Dupont Joëlle
Published: August 2019

Reactive oxygen species (ROS) which lead to oxidative stress affect ovarian function. Grape seed extract (GSE) could be proposed as an effective antioxidant, particularly due to its proanthocyanidin content. In this study, we investigated a dose effect (0, 0.01, 0.1, 1, 10, 50, and 100 μg/mL) of GSE and proanthocyanidin B2 (GSPB2) on the ROS content, cell proliferation, cell viability, and steroidogenesis in both primary luteinized granulosa cells (hGC) and the tumor granulosa cell line (KGN). The levels of ROS were measured using ROS-Glo assay. Cell proliferation and viability were evaluated by [3H]-thymidine incorporation and Cell Counting Kit-8 (CCK8) assay, respectively. Steroid secretion was evaluated by radioimmunoassay. We also analyzed the cell cycle component protein level and signaling pathways by immunoblot and the NOX4 mRNA expression by RTqPCR. From 0.1 to 1 μg/mL, GSE and GSBP2 reduced the ROS cell content and the NOX4 mRNA levels, whereas, GSE and GSBP2 increased the ROS cell content from 50 to 100 μM in both hGC and KGN. GSE and GSPB2 treatments at 50 and 100 μg/mL induced a delay in G1 to S phase cell cycle progression as determined by fluorescence-activated cell sorting. Consequently, they reduced cell growth, cyclin D2 amount, and Akt phosphorylation, and they increased protein levels of p21 and p27 cyclin-dependent kinase inhibitors. These data were also associated with an increase in cell death that could be due to a reduction in Bcl-2-associated death promoter (BAD) phosphorylation and an increase in the cleaved-caspase-3 level. All these negative effects were not observed at lower concentrations of GSE and GSPB2 (0.01 to 10 μg/mL). Interestingly, we found that GSE and GSPB2 treatments (0.1 to 100 μg/mL) improved progesterone and estradiol secretion and this was associated with a higher level of the cholesterol carriers, StAR (steroidogenic acute regulatory protein), CREB (Cyclic adenosine monophosphate Response Element-binding protein), and MAPK ERK1/2 (Mitogen-Activated Protein Kinases Extracellular signal-Regulated Kinases 1/2) phosphorylation in both hGC and KGN cells. Taken together, GSE and GSPB2 (0.1–10 μg/mL) in vitro treatments decrease oxidative stress and increase steroidogenesis without affecting cell proliferation and viability in human granulosa cells.
Synthetic Blocks for Bone Regeneration: A Systematic Review and Meta-Analysis
Authors: Tumedei Margherita , Savadori Paolo , Del Fabbro Massimo
Published: August 2019

This systematic review is aimed at evaluating the effectiveness of synthetic block materials for bone augmentation in preclinical in vivo studies. An electronic search was performed on Pubmed, Scopus, EMBASE. Articles selected underwent risk-of-bias assessment. The outcomes were: new bone formation and residual graft with histomorphometry, radiographic bone density, soft tissue parameters, complications. Meta-analysis was performed to compare new bone formation in test (synthetic blocks) vs. control group (autogenous blocks or spontaneous healing). The search yielded 214 articles. After screening, 39 studies were included, all performed on animal models: rabbits (n = 18 studies), dogs (n = 4), rats (n = 7), minipigs (n = 4), goats (n = 4), and sheep (n = 2). The meta-analysis on rabbit studies showed significantly higher new bone formation for synthetic blocks with respect to autogenous blocks both at four-week (mean difference (MD): 5.91%, 95% confidence intervals (CI): 1.04, 10.79%, p = 0.02) and at eight-week healing (MD: 4.44%, 95% CI: 0.71, 8.17%, p = 0.02). Other animal models evidenced a trend for better outcomes with synthetic blocks, though only based on qualitative analysis. Synthetic blocks may represent a viable resource in bone regenerative surgery for achieving new bone formation. Differences in the animal models, the design of included studies, and the bone defects treated should be considered when generalizing the results. Clinical studies are needed to confirm the effectiveness of synthetic blocks in bone augmentation procedures.
Whole Genome Sequencing of Chinese White Dolphin (Sousa chinensis) for High-Throughput Screening of Antihypertensive Peptides
Authors: Jia Kuntong , Bian Chao , Yi Yunhai , Li Yanping , Jia Peng , Gui Duan , Zhang Xiyang , Lin Wenzhi , Sun Xian , Lv Yunyun , Li Jia , You Xinxin , Shi Qiong , Yi Meisheng , Wu Yuping
Published: August 2019

Chinese white dolphin (Sousa chinensis), also known as the Indo-Pacific humpback dolphin, has been classified as “Vulnerable” on the IUCN Red List of Threatened Species. It is a special cetacean species that lives in tropical and subtropical nearshore waters, with significant differences from other cetaceans. Here, we sequenced and assembled a draft genome of the Chinese white dolphin with a total length of 2.3 Gb and annotation of 18,387 protein-coding genes. Genes from certain expanded families are potentially involved in DNA replication and repairing, suggesting that they may be related to adaptation of this marine mammal to nearshore environments. We also discovered that its historical population had undergone a remarkable bottleneck incident before the Mindel glaciation. In addition, a comparative genomic survey on antihypertensive peptides (AHTPs) among five representative mammals with various residential habitats (such as remarkable differences in exogenous ion concentrations and sea depth) revealed that these small bioactive peptides were highly conserved among these examined mammals, and they had the most abundant hits in collagen subunit proteins, especially for two putative AHTP peptides Gly-Leu-Pro (GLP) and Leu-Gly-Pro (LGP). Our genome assembly will be a valuable resource for further genetic researches on adaptive ecology and conservation biology of cetaceans, and for in-depth investigations into bioactive peptides in aquatic and terrestrial mammals for development of peptide-based drugs to treat various human cardiovascular diseases.
Antioxidant Fucoidans Obtained from Tropical Seaweed Protect Pre-Osteoblastic Cells from Hydrogen Peroxide-Induced Damage
Authors: Fidelis Gabriel Pereira , Silva Cynthia Haynara Ferreira , Nobre Leonardo Thiago Duarte Barreto , Medeiros Valquíria Pereira , Rocha Hugo Alexandre Oliveira , Costa Leandro Silva
Published: August 2019

Some antioxidant compounds decrease the amount of intracellular reactive oxygen species (ROS) and consequently reduce the deleterious effects of ROS in osteoblasts. Thus, these compounds fight against osteoporosis. Brown seaweeds are a rich source of antioxidant fucose-containing sulfated polysaccharides (fucans and fucoidans). We obtained six fucoidans (FRFs)—F0.3, F0.5, F0.7, F1.0, F1.5, and F2.1—from Dictyota mertensii by proteolytic digestion followed by sequential acetone precipitation. Except for F0.3, all FRFs showed antioxidant activity in different in vitro tests. In pre- osteoblast-like cells (MC3T3-L1) exposed to H2O2-oxidative stress, caspase-3 and caspase-9 were activated, resulting in apoptosis of the cells. We also observed a decrease in superoxide dismutase (SOD) and alkaline phosphatase (ALP) activity. The antioxidant FRFs protected the cells from the oxidative damage caused by H2O2, decreasing intracellular ROS and caspase activation, and increasing SOD activity. The most effective protection against damage was provided by F0.7, F1.5, and F2.1. At 0.5 mg/mL, these FRFs also suppressed the H2O2-mediated inhibition of ALP activity. The data indicated that FRFs F0.7, F1.5, and F2.1 from D. mertensii were antioxidants that protected bone tissue from oxidative stress and could represent possible adjuvants for the treatment of bone fragility through counteracting oxidative phenomena.
Using pH-Activable Carbon Nanoparticles as Cell Imaging Probes
Authors: Lin Honggui , Su Jianlong , Kankala Ranjith Kumar , Zeng Mingrong , Zhou Shu-Feng , Lin Xuexia
Published: August 2019

Herein, we demonstrate the fabrication of innovative pH-activable carbon nanoparticles (CNPs) based on urea and citric acid by microwave-assisted green synthesis for application in cell imaging. These CNP-based nanoprobes offer significant advantages of pH responsiveness and excellent biocompatibility. The pH responsiveness ranges from 1.0 to 4.6 and the slightly pH responsiveness ranges from 4.6 to 9.0. In addition, the pH-dependent modification of charge as well as the final diameter of the designed CNPs not only provide support as stable sensors for cell imaging under pH values from 4.6 to 9.0, but can also observe the pH change in cells from 1.0 to 4.6. Importantly, this significantly enhances the cellular internalization process resulting in tumor cell death. Together, we believe that these superior photoluminescence properties of our designed nanomaterials potentially allow for biological labeling, bioimaging, and drug delivery applications.
Comprehensive Provenance Analysis and Its Applications to Eocene Clastic Rocks in the Huimin Depression, Bohai Bay Basin, China
Authors: Zhang Zehua , Wang Hongliang
Published: August 2019

Clarification of the source of the Eocene sediments filling the Huimin depression provides significant support for current and future oil and gas exploration in the Bohai Bay Basin, China. A comprehensive assessment of sediment provenance based on sandstone petrography, heavy mineral assemblages, seismic reflection data, and distribution of sandstone bodies of the Shahejie Formation (Es2) to the Dongying Formation (Ed) productive interval based on both its macroscopic and microscopic provenance aspects. This method of analysis has the advantages of easy data acquisition, high accuracy, strong flexibility, wide application range, and making the analysis of sedimentary provenances more systematic. Comprehensive analysis of the Huimin depression, Bohai Bay Basin has revealed further that the origin of the Eocene second member of the Shahejie Formation (Es2) to the Dongying Formation (Ed) provenance system was derived from the Linfanjia high, the Chengning uplift, and the Luxi uplift.
A DFT Approach to the Surface-Enhanced Raman Scattering of 4-Cyanopyridine Adsorbed on Silver Nanoparticles
Authors: López-Tocón Isabel , Valdivia Samuel , Soto Juan , Otero Juan Carlos , Muniz-Miranda Francesco , Menziani Maria Cristina , Muniz-Miranda Maurizio
Published: August 2019

A Surface-Enhanced Raman Scattering (SERS) spectrum of 4-cyanopyridine (4CNPy) was recorded on silver plasmonic nanoparticles and analyzed by using Density Functional Theory (DFT) calculations. Two simple molecular models of the metal–4CNPy surface complex with a single silver cation or with a neutral dimer (Ag+–4CNPy, Ag2–4CNPy), linked through the two possible interacting sites of 4CNPy (aromatic nitrogen, N, and nitrile group, CN), were considered. The calculated vibrational wavenumbers and intensities of the adsorbate and the isolated species are compared with the experimental Raman and SERS results. The analysis of the DFT predictions and the experimental data indicates that 4CNPy adsorbs preferentially on neutral/charged active sites of the silver nanoparticles through the nitrogen atom of the aromatic ring with a perpendicular orientation.
Biocompatibility of Cyclopropylamine-Based Plasma Polymers Deposited at Sub-Atmospheric Pressure on Poly (ε-caprolactone) Nanofiber Meshes
Authors: Chan Ke Vin , Asadian Mahtab , Onyshchenko Iuliia , Declercq Heidi , Morent Rino , De Geyter Nathalie
Published: August 2019

In this work, cyclopropylamine (CPA) monomer was plasma-polymerized on poly (ε-caprolactone) nanofiber meshes using various deposition durations to obtain amine-rich surfaces in an effort to improve the cellular response of the meshes. Scanning electron microscopy and X-ray photoelectron spectroscopy (XPS) were used to investigate the surface morphology and surface chemical composition of the PCL samples, respectively. The measured coating thickness was found to linearly increase with deposition duration at a deposition rate of 0.465 nm/s. XPS analysis revealed that plasma exposure time had a considerable effect on the surface N/C and O/C ratio as well as on amino grafting efficiency and amino selectivity. In addition, cell studies showed that cell adhesion and proliferation significantly improved for all coated samples.
Antibacterial Nanostructured Ti Coatings by Magnetron Sputtering: From Laboratory Scales to Industrial Reactors
Authors: Alvarez Rafael , Muñoz-Piña Sandra , González María U. , Izquierdo-Barba Isabel , Fernández-Martínez Iván , Rico Víctor , Arcos Daniel , García-Valenzuela Aurelio , Palmero Alberto , Vallet-Regi María , González-Elipe Agustín R. , García-Martín José M.
Published: August 2019

Based on an already tested laboratory procedure, a new magnetron sputtering methodology to simultaneously coat two-sides of large area implants (up to ~15 cm2) with Ti nanocolumns in industrial reactors has been developed. By analyzing the required growth conditions in a laboratory setup, a new geometry and methodology have been proposed and tested in a semi-industrial scale reactor. A bone plate (DePuy Synthes) and a pseudo-rectangular bone plate extracted from a patient were coated following the new methodology, obtaining that their osteoblast proliferation efficiency and antibacterial functionality were equivalent to the coatings grown in the laboratory reactor on small areas. In particular, two kinds of experiments were performed: Analysis of bacterial adhesion and biofilm formation, and osteoblasts–bacteria competitive in vitro growth scenarios. In all these cases, the coatings show an opposite behavior toward osteoblast and bacterial proliferation, demonstrating that the proposed methodology represents a valid approach for industrial production and practical application of nanostructured titanium coatings.
Taste Receptors in Upper Airway Innate Immunity
Authors: Carey Ryan M. , Lee Robert J.
Published: August 2019

Taste receptors, first identified on the tongue, are best known for their role in guiding our dietary preferences. The expression of taste receptors for umami, sweet, and bitter have been demonstrated in tissues outside of the oral cavity, including in the airway, brain, gastrointestinal tract, and reproductive organs. The extra-oral taste receptor chemosensory pathways and the endogenous taste receptor ligands are generally unknown, but there is increasing data suggesting that taste receptors are involved in regulating some aspects of innate immunity, and may potentially control the composition of the nasal microbiome in healthy individuals or patients with upper respiratory diseases like chronic rhinosinusitis (CRS). For this reason, taste receptors may serve as potential therapeutic targets, providing alternatives to conventional antibiotics. This review focuses on the physiology of sweet (T1R) and bitter (T2R) taste receptors in the airway and their activation by secreted bacterial products. There is particular focus on T2R38 in sinonasal ciliated cells, as well as the sweet and bitter receptors found on specialized sinonasal solitary chemosensory cells. Additionally, this review explores the impact of genetic variations in these receptors on the differential susceptibility of patients to upper airway infections, such as CRS.
Changes in Plasma Free Fatty Acids Associated with Type-2 Diabetes
Authors: I. S. Sobczak Amélie , A. Blindauer Claudia , J. Stewart Alan
Published: August 2019

Type 2 diabetes mellitus (T2DM) is associated with increased total plasma free fatty acid (FFA) concentrations and an elevated risk of cardiovascular disease. The exact mechanisms by which the plasma FFA profile of subjects with T2DM changes is unclear, but it is thought that dietary fats and changes to lipid metabolism are likely to contribute. Therefore, establishing the changes in concentrations of specific FFAs in an individual’s plasma is important. Each type of FFA has different effects on physiological processes, including the regulation of lipolysis and lipogenesis in adipose tissue, inflammation, endocrine signalling and the composition and properties of cellular membranes. Alterations in such processes due to altered plasma FFA concentrations/profiles can potentially result in the development of insulin resistance and coagulatory defects. Finally, fibrates and statins, lipid-regulating drugs prescribed to subjects with T2DM, are also thought to exert part of their beneficial effects by impacting on plasma FFA concentrations. Thus, it is also interesting to consider their effects on the concentration of FFAs in plasma. Collectively, we review how FFAs are altered in T2DM and explore the likely downstream physiological and pathological implications of such changes.
Functionalized Poly(arylene ether nitrile) Porous Membrane with High Pb(II) Adsorption Performance
Authors: Liu Xiaocan , Zhou Meirong , Zhou Xuefei , Wang Lingling , Liu Xiaobo
Published: August 2019

Porous materials with high specific surface area possess a broad application prospect in the treatment of wastewater. In this work, sulfonated poly(arylene ether nitrile) (SPEN) functionalized with a carboxylic acid group was successfully synthesized, which was subsequently transformed into SPEN porous membranes with cetyltrimethyl ammonium bromide (CTAB) as pore-forming agents to study the adsorption performance for lead ions in aqueous solution. Then, experiments were conducted to investigate the effect of pH, contact time and initial solution concentration on the adsorption performance of porous membranes, and the adsorption capacities of porous membranes with different content (0, 5 and 15 wt %) of CTAB were 183.60, 161.73 and 127.43 mg/g, respectively, which manifested that the adsorption capacity decreased with the increase of CTAB. The adsorption capacities of porous membranes increased with the increase of the initial concentration of lead ions, and the maximum reached was 246.96 mg/g. The simulation of adsorption kinetics revealed that the adsorption was accorded with the pseudo-second-order kinetic model and Langmuir equation, indicating that the adsorption process followed Langmuir monolayer adsorption. Thermogravimetric analysis demonstrated that the porous membranes had excellent thermodynamic properties both before and after adsorption. In addition, the change of adsorption peak in the Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) spectrum indicated that the absorption performance of porous membranes for lead ions benefited from the chelation between lead ions and the carboxylic acid group on SPEN. Moreover, the porous membranes maintained excellent adsorption properties after circulating five times under the conditions of acidic or alkaline, and the cycle regeneration effect was outstanding.
Creaturely Communal Ontology in Practice: John Zizioulas in Dialogue with Ritual Theory
Authors: Compton John W.
Published: August 2019

This article is born out of a deep concern for our current ecological crisis and serves as a beginning foundational work for how the Christian tradition can address global climate change. Our current way of being gives precedence to the autonomous individual, whose freedom is characterized by disregard for other creatures. John Zizioulas’ communal ontology demonstrates that as the world was created out of God’s loving will, it is comprised of relationship. Living into individuation and division is a refusal of this communion with other creatures and God, but the Eucharist serves as the ritual that brings Christians into communion through the remembrance of Christ. Ian McFarland’s work on the theology of creation provides the helpful nuance that creaturely movement in communion must include the full diversity of creatures. I then turn to Bruce Morrill’s work to demonstrate that the Eucharistic practice must have bearing beyond the walls of the church. It leads practitioners to live into eschatological hope and kenotic service to the world. John Seligman’s ritual theory demonstrates that ritual practice can accomplish these goals because it creates a subjunctive ‘as-if’ world in the face of the world that is perceived as chaotic. Through the continuous practice of the ritual, participants are then formed to live into this subjunctive ‘as-if’ world without ritual precedence. In this way, the Eucharistic practice can prepare practitioners to live into the kenotic service to a world broken by individuation that has led to global climate change and creaturely destruction.
Impact of Signal Quantization on the Performance of RFI Mitigation Algorithms
Authors: Díez-García Raúl , Camps Adriano
Published: August 2019

Radio Frequency Interference (RFI) is currently a major problem in Communications and Earth Observation, but it is even more dramatic in Microwave Radiometry because of the low power levels of the received signals. Its impact has been attested in several Earth Observation missions. On-board mitigation systems are becoming a requirement to detect and remove affected measurements, increasing thus radiometric accuracy and spatial coverage. However, RFI mitigation methods have not been tested yet in the context of some particular radiometer topologies, which rely on the use of coarsely quantized streams of data. In this study, the impact of quantization and sampling in the performance of several known RFI mitigation algorithms is studied under different conditions. It will be demonstrated that in the presence of clipping, quantization changes fundamentally the time-frequency properties of the contaminated signal, strongly impairing the performance of most mitigation methods. Important design considerations are derived from this analysis that must be taken into account when defining the architecture of future instruments. In particular, the use of Automatic Gain Control (AGC) systems is proposed, and its limitations are discussed.
Low-Cost Radon Detector with Low-Voltage Air-Ionization Chamber
Authors: Studnička Filip , Štěpán Jan , Šlégr Jan
Published: August 2019

This paper describes the design of a low-cost radon detector that can easily be fabricated in large quantities for the purposes of earthquake prediction. The described detector can also be used for monitoring radon levels in houses because high radon levels pose a great health risk. A very simple air-ionization chamber for alpha particles was used, considering the experimental results. Chamber current-sensing circuitry is also suggested, and an Internet of Things (IoT) sensor grid is described. The main advantages of this detector are the low cost, low power consumption, and complete elimination of high-voltage power sources. The minimum detectable activity achieved with the proposed detector for one measurement was around 50 Bq · m − 3 , with time of measurement comparable to that featured on commercial devices, while the price of the described detector is one order of magnitude lower.
A Method of Short Text Representation Based on the Feature Probability Embedded Vector
Authors: Zhou Wanting , Wang Hanbin , Sun Hongguang , Sun Tieli
Published: August 2019

Text representation is one of the key tasks in the field of natural language processing (NLP). Traditional feature extraction and weighting methods often use the bag-of-words (BoW) model, which may lead to a lack of semantic information as well as the problems of high dimensionality and high sparsity. At present, to solve these problems, a popular idea is to utilize deep learning methods. In this paper, feature weighting, word embedding, and topic models are combined to propose an unsupervised text representation method named the feature, probability, and word embedding method. The main idea is to use the word embedding technology Word2Vec to obtain the word vector, and then combine this with the feature weighted TF-IDF and the topic model LDA. Compared with traditional feature engineering, the proposed method not only increases the expressive ability of the vector space model, but also reduces the dimensions of the document vector. Besides this, it can be used to solve the problems of the insufficient information, high dimensions, and high sparsity of BoW. We use the proposed method for the task of text categorization and verify the validity of the method.
Assessment of Ultra-Short Heart Variability Indices Derived by Smartphone Accelerometers for Stress Detection
Authors: Landreani Federica , Faini Andrea , Martin-Yebra Alba , Morri Mattia , Parati Gianfranco , Caiani Enrico Gianluca
Published: August 2019

Body acceleration due to heartbeat-induced reaction forces can be measured as mobile phone accelerometer (m-ACC) signals. Our aim was to test the feasibility of using m-ACC to detect changes induced by stress by ultra-short heart rate variability (USV) indices (standard deviation of normal-to-normal interval—SDNN and root mean square of successive differences—RMSSD). Sixteen healthy volunteers were recruited; m-ACC was recorded while in supine position, during spontaneous breathing at rest conditions (REST) and during one minute of mental stress (MS) induced by arithmetic serial subtraction task, simultaneous with conventional electrocardiogram (ECG). Beat occurrences were extracted from both ECG and m-ACC and used to compute USV indices using 60, 30 and 10 s durations, both for REST and MS. A feasibility of 93.8% in the beat-to-beat m-ACC heart rate series extraction was reached. In both ECG and m-ACC series, compared to REST, in MS the mean beat duration was reduced by 15% and RMSSD decreased by 38%. These results show that short term recordings (up to 10 s) of cardiac activity using smartphone’s accelerometers are able to capture the decrease in parasympathetic tone, in agreement with the induced stimulus.
Limitations of Water Resources to Crop Water Requirement in the Irrigation Districts along the Lower Reach of the Yellow River in China
Authors: Liu Lei , Ma Jianqin , Hao Xiuping , Li Qingyun
Published: August 2019

To analyze the water-resource limitations for crops in irrigation districts along the lower reach of the Yellow River, we used the single-crop coefficient method provided by FAO-56 to analyze crop water demand (CWD) and irrigation water requirement (IWR) for the main crops (winter wheat, summer maize, and cotton) from 1971 to 2015. The impact of climate threats on IWR was then quantified based on the standardized precipitation evapotranspiration index (SPEI), following which the conflicts between water demand and water supply were analyzed. The results show that about 75.4% of the total annual IWR volume is concentrated from March to June. Winter wheat is the largest water consumer; it used an average of 67.9% of the total IWR volume. The study area faced severe water scarcity, and severe water deficits occurred mainly between March and June, which is consistent with the occurrence of drought. With the runoff from the Yellow River Basin further decreasing in the future, the water supply is expected to become more limited. IWR is negatively correlated with the SPEI. Based on the relationship between SPEI and IWR, the water allocation for irrigation can be planned at different timescales to meet the CWD of different crops.
Liquid Biphasic Systems for Oil-Rich Algae Bioproducts Processing
Authors: Leong Hui Yi , Chang Chih-Kai , Lim Jun Wei , Show Pau Loke , Lin Dong-Qiang , Chang Jo-Shu
Published: August 2019

Oleaginous algae are nowadays of significance for industrial biotechnology applications and for the welfare of society. Tremendous efforts have been put into the development of economically feasible and effective downstream processing techniques in algae research. Currently, Liquid Biphasic Systems (LBSs) are receiving much attention from academia and industry for their potential as green and effective downstream processing methods. This article serves to review the applications of LBSs (LBS and Liquid Biphasic Flotation System (LBFS)) in the separation, recovery and purification of algae products, as well as their basic working principles. Moreover, cell disruptive technologies incorporated into LBSs in algae research are reported. This review provides insights into the downstream processing in algae industrial biotechnology which could be beneficial for algae biorefinement.
Radon Gas as an Indicator for Air Quality Control in Buried Industrial Architecture: Rehabilitation of the Old Británica Warehouses in Alicante for a Tourist Site
Authors: Rizo-Maestre Carlos , Echarri-Iribarren Víctor , Prado-Govea Raúl , Pujol-López Francisco
Published: August 2019

The infrastructure of the Británica warehouses in Alicante is a very important industrial architectural element in the history of Spain, although it is unknown to almost all of the inhabitants of the city. The former fuel refinery is located in the Serra Grossa Mountains and served much of the country until 1966. This research is based on the plans of the city of Alicante to convert a historical element, the Británica warehouses, into a unique tourist site. Currently, the network of storage domes in this facility, which has an approximate footprint of 20,000 m2 and domes approximately 20 m high, is in a state of neglect, and there are neighborhood initiatives for its rehabilitation to become a cultural or tourist site. Therefore, it is necessary to take into account the quality of the indoor air. Radon gas is analyzed as a control element for future refurbishment of the facility. Alicante is a nongranite area and therefore is not very susceptible to generation of radon gas indoors, but the conditions of a buried and poorly ventilated space make the site appropriate for analysis. Most scientific agencies in the field of medicine and health, including the World Health Organization, consider radon gas to be very harmful to humans. This element in its gaseous state is radioactive and is present in almost all the land in which the buildings are implanted, with granitic type soils presenting higher levels of radon gas. Nongranitic soils have traditionally been considered to have low radon levels. The city of Alicante, where the installation is located, is a nongranitic area and therefore is not very susceptible to generating radon gas in buildings, but the conditions of buried and poorly ventilated places make the site appropriate for analysis to support air quality control and decision-making.
Prototiles and Tilings from Voronoi and Delone Cells of the Root Lattice An
Authors: Ozdes Koca Nazife , Al-Siyabi Abeer , Koca Mehmet , Koc Ramazan
Published: August 2019

The orthogonal projections of the Voronoi and Delone cells of root lattice A n onto the Coxeter plane display various rhombic and triangular prototiles including thick and thin rhombi of Penrose, Amman–Beenker tiles, Robinson triangles, and Danzer triangles to name a few. We point out that the symmetries representing the dihedral subgroup of order 2 h involving the Coxeter element of order h = n + 1 of the Coxeter–Weyl group a n play a crucial role for h -fold symmetric tilings of the Coxeter plane. After setting the general scheme we give samples of patches with 4-, 5-, 6-, 7-, 8-, and 12-fold symmetries. The face centered cubic (f.c.c.) lattice described by the root lattice A 3 , whose Wigner–Seitz cell is the rhombic dodecahedron projects, as expected, onto a square lattice with an h = 4 -fold symmetry.
Impact of integrating pharmacists into primary care teams on health systems indicators: a systematic review
Authors: Hayhoe Benedict , Cespedes Jose Acuyo , Foley Kimberley , Majeed Azeem , Ruzangi Judith , Greenfield Geva
Published: August 2019

Background

Evidence suggests that pharmacists integrated into primary care can improve patient outcomes and satisfaction, but their impact on healthcare systems is unclear.

Aim

To identify the key impacts of pharmacists’ integration into primary care on health system indicators, such as healthcare utilisation and costs.

Design and setting

A systematic review of literature.

Method

Embase, MEDLINE, Scopus, the Health Management Information Consortium, CINAHL, and the Cochrane Central Register of Controlled Trials databases were examined, along with reference lists of relevant studies. Randomised controlled trials (RCTs) and observational studies published up until June 2018, which considered health system outcomes of the integration of pharmacists into primary care, were included. The Cochrane risk of bias quality assessment tool was used to assess risk of bias for RCTs; the National Institute of Health National Heart, Lung, and Blood Institute quality assessment tool was used for observational studies. Data were extracted from published reports and findings synthesised.

Results

Searches identified 3058 studies, of which 28 met the inclusion criteria. Most included studies were of fair quality. Pharmacists in primary care resulted in reduced use of GP appointments and reduced emergency department (ED) attendance, but increased overall primary care use. There was no impact on hospitalisations, but some evidence of savings in overall health system and medication costs.

Conclusion

Integrating pharmacists into primary care may reduce GP workload and ED attendance. However, further higher quality studies are needed, including research to clarify the cost-effectiveness of the intervention and the long-term impact on health system outcomes.
GP incentives to design hypertension and atrial fibrillation local quality-improvement schemes: a controlled before–after study in UK primary care
Authors: Smith Timothy , Fell Christopher , Otete Harmony , Chauhan Umesh
Published: August 2019

Background

Financial incentives in the UK such as the Quality and Outcomes Framework (QOF) reward GP surgeries for achievement of nationally defined targets. These have shown mixed results, with weak evidence for some measures, but also possible unintended negative effects.

Aim

To look at the effects of a local intervention for atrial fibrillation (AF) and hypertension, with surgeries rewarded financially for work, including appointing designated practice leads, attendance at peer review workshops, and producing their own protocols.

Design and setting

A controlled before–after study comparing surgery performance measures in UK primary care.

Method

This study used published QOF data to analyse changes from baseline in mean scores per surgery relating to AF and hypertension prevalence and management at T1 (12 months) and T2 (24 months) for the intervention group, which consisted of all 58 surgeries in East Lancashire Clinical Commissioning Group (CCG), compared to the control group, which consisted of all other surgeries in north-west England.

Results

There was a small acceleration between T0 (baseline) and T2 in recorded prevalence of hypertension in the intervention group compared to the controls, difference 0.29% (95% confidence interval [CI] = 0.05 to 0.53), P = 0.017, but AF prevalence did not increase more in the intervention group. Improvement in quality of management of AF was significantly better in the intervention group, difference 3.24% (95% CI = 1.37 to 5.12), P = 0.001.

Conclusion

This intervention improved diagnosis rates of hypertension but not AF, though it did improve quality of AF management. It indicates that funded time to develop quality-improvement measures targeted at a local population and involving peer support can engage staff and have the potential to improve quality.
Mechanism of DNA Lesion Homing and Recognition by the Uvr Nucleotide Excision Repair System
Authors: Lee Seung-Joo , Sung Rou-Jia , Verdine Gregory L.
Published: August 2019

Nucleotide excision repair (NER) is an essential DNA repair system distinguished from other such systems by its extraordinary versatility. NER removes a wide variety of structurally dissimilar lesions having only their bulkiness in common. NER can also repair several less bulky nucleobase lesions, such as 8-oxoguanine. Thus, how a single DNA repair system distinguishes such a diverse array of structurally divergent lesions from undamaged DNA has been one of the great unsolved mysteries in the field of genome maintenance. Here we employ a synthetic crystallography approach to obtain crystal structures of the pivotal NER enzyme UvrB in complex with duplex DNA, trapped at the stage of lesion-recognition. These structures coupled with biochemical studies suggest that UvrB integrates the ATPase-dependent helicase/translocase and lesion-recognition activities. Our work also conclusively establishes the identity of the lesion-containing strand and provides a compelling insight to how UvrB recognizes a diverse array of DNA lesions.
Fine-scale to Flora-wide Phylogenetic Perspectives on Californian Plant Diversity, Endemism, and Conservation
Authors: Baldwin Bruce G.
Published: August 2019

The California Floristic Province (CA-FP) encompasses North America’s Mediterranean-type climatic region and is a global biodiversity hotspot in temperate North America. Fine-scale phylogenetic studies and increasing scrutiny of morphological, ecological, and geographic variation in the CA-FP vascular flora continue to reveal novel, often cryptic diversity warranting taxonomic attention. The prevalence of such discoveries warrants caution about potential for loss of undescribed or unrecognized biodiversity from human-related impacts, including insufficiently informed conservation-related actions (e.g., genetic augmentation or assisted migration). Broader-scale molecular phylogenetic studies have yielded other examples of more extensive CA-FP diversification than previously resolved. For example, clades uniting CA-FP taxa not earlier thought to be such close relatives have been discovered in a wide diversity of tribes of composites and legumes. This understanding adds additional weight to the conclusions of Raven and Axelrod (1978) that in situ evolution, especially since the pronounced mid-Miocene transition toward summer-drying, has been a major factor in the origins of Californian vascular plant diversity. The importance of paleo-endemism in gymnosperms and (especially woody) angiosperms of the CA-FP flora also has been corroborated and refined by molecular phylogenetic studies, with improved understanding of the timing of divergence and relationships of isolated lineages, such as the extinct island-endemic Hesperelaea A. Gray (Oleaceae). On a regional scale, studies of spatial patterns of Californian species richness, phylogenetic diversity, and phylogenetic endemism by the California Plant Phylodiversity Project (CPPP) have reinforced the floristic importance of areas of high topographic complexity, or environmental heterogeneity more generally, where relatively high levels of habitat diversity, speciation, and lineage persistence may be expected. The CPPP’s finding that particularly young plant lineages and significantly high phylogenetic endemism are concentrated in drier regions of the CA-FP and in the adjacent Californian deserts has corroborated earlier preliminary findings and aligns with Stebbins’s (1952) hypothesis of aridity as an evolutionary stimulus. A recent conservation gap analysis by the CPPP incorporating flora-wide phylogenetic and geographic occurrence data plus land-protection status and habitat-integrity information for California has demonstrated the potential of an integrative, evolutionary approach for identifying high-priority land conservation targets.
Where coasts and rivers meet: Living on the edge requires us all to work together
Authors: Cunniff Shannon
Published: August 2019

By recognizing the interconnected nature of our coastal and riparian systems — the physical, economic, and social systems — we can make progress toward creating and implementing better approaches to realize communities that are vibrant and increasingly resilient to higher sea levels and storm surges, inland flooding, erosion, and myriad other climate-related changes.
Persepsi Peserta Didik terhadap Metode Blended Learning dengan Google Classroom
Authors: Endah Wulantina Sugama Maskar,
Published: August 2019

Revolusi Industri 4.0 mendorong perubahan pada proses pendidikan dan pembelajaran daring merupakan salah satu tantangan yang harus dihadapi. Blended Learning merupakan salah satu solusi untuk menuju perubahan tersebut. Tujuan penelitian ini adalah untuk mengetahui sikap siswa terhadap proses pembelajaran berbasis Blended Learning pada sampel kelas Statistika di Universitas Teknokrat Indonesia. Proses pembelajaran daring dilakukan dengan menggunakan Google Classroom. Teknik pengumpulan dan analisa data menggunakan angket skala sikap yang terdiri dari dua variabel, sikap terhadap proses pembelajaran dan sikap terhadap pemahaman materi, yang didukung oleh 9 indikator dan 21 pernyataan. Hasil penelitian ini menunjukan bahwa peserta didik merasa proses pembelajaran dengan Metode Blended Learning melalui Google Classroom ini membuat proses pembelajaran menjadi menarik, efektif, menumbuhkan motivasi, menumbuhkan sikap belajar mandiri, aktif, dan kreatif. Selain itu, metode ini juga dapat meningkatkan pemahaman dan hasil belajar peserta didik. Namun, peserta didik merasa proses pembelajaran menjadi tidak efisien karena mengharuskan mereka mempunyai paket data. Oleh karena itu, peran pemerintah dirasa sangat penting, salah satunya dalam hal pengadaan sarana dan prasarana untuk mendukung proses pembelajaran Blended Learning agar dapat memudahkan peserta didik untuk tetap terhubung dengan internet secara gratis.
Development of the Strategic Framework for Sustainable Development of the Special Economic Zones in ASEAN Economic Community: A Case Study of Savannakhet - Mukdahan
Authors: Siwapimon Kantachon , Sriburi Thavivongse
Published: August 2019

The study proposes a strategic sustainable development (SD) framework for the special economic zones in the ASEAN Economic Community (AEC) by investigating 11 key factors to the success of SD, using the concepts of Strategic Environment Assessment (SEA) and Sustainability Assessment (SA). The key factors are 1) public education, 2) educational system, 3) international SDstandard, 4) policy, 5) management structure, 6) environmental management, 7) zoning, 8) expenditure, 9) public participation, 10) public health and 11) local business protection. The study use qualitative methods including a literature review as well as collection of primary data through a semi-structured interview form by face-to-face in-depth interviewing with 56 stakeholders selected through the triangulation and appreciative inquiry techniques. The data were qualitatively analyzed to present opinions, problems, needs and results according to SEA and SA. Using content analysis, an analysis was conducted of the state of SD in the target areas of Mukdahan and Savannakhet special economic zones. A strategic SD framework was designed for use in the AEC region. The study recommends further investigation of key factors to identify those that may be specific to each area.
Investigation of Formaldehyde in Gross Anatomy Laboratory: Area-Based and Exposure Levels, Ventilation, Health Risk and Clinical Symptoms
Authors: Homwutthiwong Kritsana , Ongwandee Maneerat
Published: August 2019

Formaldehyde is commonly used in embalming solution in medical fields to preserve tissues. Formaldehyde vapor released from cadavers during dissection practice can adversely affect students and instructors in gross anatomy laboratories. Therefore, this study investigated formaldehyde concentrations in a gross anatomy laboratory of Mahasarakham University, Thailand. Area-based sampling was conducted for three scenarios: (1) a laboratory cleaning period, (2) three periods of teaching classes and (3) a non-teaching class period. Personal samples were also collected for five consecutive working days from two anatomy lab instructors and a non-lab instructor. Measurements were conducted during May to October 2016 using cartridges filled with dinitrophenylhydrazine coated silica gel. Samples were desorbed and analyzed by high performance liquid chromatography. Results showed that the average area-based concentrations during the dissection classes ranged from 9.3 to 17.6 ppb, while the non-teaching class concentration was 5.5 ppb. The concentration increased to 3.1×103 ppb during the laboratory cleaning. The five-day average exposure concentrations were 7.6 ppb and 4.7 ppb for the two lab instructors and 1.1 ppb for the non-lab instructor. The lifetime cancer risk estimates were 5.8×10-6, 2.9×10-6 and 8.1×10-7, respectively. The three most reported clinical symptoms by the gross anatomy students were associated with formaldehyde concentrations above 16 ppb at the significance level of 0.05. These included unpleasant odor, general fatigue or fatigue after awakening and dizziness with response rates of 57.5 %, 38.5 % and 33.1 %, respectively. Improvement of the ventilation and source control measures are essential for reducing formaldehyde emissions in the gross anatomy laboratory.
Effect of Hydrological Cycle on Climate
Authors: Sung Lian
Published: August 2019

The water cycle, also known as the hydrological cycle or the hydrologic cycle, describes the continuous movement of water on, above and below the surface of the Earth. The mass of water on Earth remains constant over time but the partitioning of the water into the major reservoirs of ice, fresh water, saline water and atmospheric water is variable depending on a wide range of climatic variables. The water moves from one reservoir to another, such as from river to ocean, or from the ocean to the atmosphere, by the physical processes of evaporation, condensation, precipitation, infiltration, surface runoff, and subsurface flow. In doing so, the water goes through different forms: liquid, solid (ice) and vapor.
Child and Their Development
Authors: Agnew Rebecca
Published: August 2019

We know that what is life. Being child is a period of life circle. If you want to know that what about child, then the term development comes automatically. Chide development is an important matter. There many problems become while developing a child. In this article, I will describe these points thoroughly. This discussion thus will be an important discussion about child.