bluuebunny/crossref_metadata_2025 huggingface dataset:
Offset Voltage Reduction in Two-Stage Folded-Cascode Operational Amplifier Using High-Precision Source Degeneration
Authors: Stancu Cristian , Neacsu Andrei , Ionescu Teodora , Stanescu Cornel , Profirescu Ovidiu , Dobrescu Dragos , Dobrescu Lidia
Published: November 2023

The demand for CMOS precision operational amplifiers for critical applications has continuously increased over time due to higher accuracy and sensitivity requirements. Trimming or chopper architectures are advanced solutions that reduce the offset voltage and improve the circuit’s parameters, but the complexity and the increased chip die size are serious downsides. An efficient solution is a source degeneration configuration to control the transistor’s current-mirror transconductance, which impacts the offset voltage, with cost savings and a die area reduction also obtained. This paper focuses on designing and implementing such an approach in a two-stage folded-cascode operational amplifier. State-of-the-art thin-film resistors that use silicon–chromium as the metallic alloy were implemented to reduce mismatch variations between these passive components. Distinct methods that control the offset voltage parameter are also discussed and established. A comparison between the offset voltage standard deviation obtained using different types of resistors and that achieved with the innovative high-precision resistors was also carried out. The source degeneration’s impact on the common-mode rejection ratio, power supply rejection ratio, bandwidth and phase margin was also analyzed, and a comparison between the proposed design and the classical one was performed. The process variation’s influence on the circuit functionality was studied. A pre-layout ±1.273 mV maximum offset voltage at T = 27 °C was achieved using vector/array notations for the amplifier with the best overall performance. Post-layout simulations that included parasitic effects were performed, with a ±1.254 mV maximum offset voltage reached at room temperature.
Economic Analysis of Large-Scale Renewable Energy (RE) Source Investment Incorporating Power System Transmission Costs
Authors: Lee Deukyoung , Joo Sung-Kwan
Published: November 2023

Recently, the proportion of renewable energy (RE) sources in a power system has been increasing worldwide to reduce carbon emissions. To effectively accommodate renewable energy, there is a growing need to develop integrated planning strategies for both renewable energy sources and transmission lines in a power system, taking into account the location-specific characteristics of renewable energy. Economically viable and rapid system expansion plans are required to resolve the problem of delayed integration of renewable energy into existing power system, which arises due to inadequate transmission facilities. To address these problems, this paper presents an integrated economic evaluation method that considers the inherently uncertain output characteristics of renewable energy, contingent on location, the costs associated with installing transmission lines, and environmental benefits. The case study is conducted using a modified power system in Korea. In the case study, it is assumed that by integrating solar power generators in a low-demand area and wind power generators in a high-demand area, wind power plants have higher construction costs than solar power generators. However, the transmission costs for wind power plants are lower than those for solar power generators due to the regional characteristics of the power grid in the installation areas because renewable energy in high-demand areas typically requires less additional power infrastructure than in low-demand areas. The results of this study show that the net benefit for the wind power plant is estimated at USD 225.4 M, while the solar power plant yields a net benefit of USD 22.9 M in the example system. These results demonstrate the effectiveness of the proposed method, underscoring its potential to mitigate the challenges faced in the practical implementation of renewable energy projects.
Machine Learning-Based Energy Consumption Estimation of Wastewater Treatment Plants in Greece
Authors: Karadimos Panagiotis , Anthopoulos Leonidas
Published: November 2023

Amidst a global discourse on energy resources, it is imperative to provide decision makers with a comprehensive overview of energy consumption (EC) associated with various projects, particularly wastewater treatment plants (WWTPs). Ensuring compliance with stringent effluent quality criteria in the treatment of municipal wastewater necessitates a substantial EC, representing a predominant factor contributing to the operational expenses incurred by WWTP. Machine learning (ML) techniques can contribute to the estimation of the WWTPs’ EC, which requires efficient and accurate data. This article uses data from several municipal WWTP projects in Greece, which are examined in order to produce EC estimation models. Data were first statistically analyzed, according to the context of project attributes and the context of EC, and correlation analysis identified the appropriate predictive project variables. Then, the attribute selection function in Waikato Environment for Knowledge Analysis 3.8.4 (WEKA 3.8.4) software emphasized the most effective subset of variables. The extracted variables from the combination of the correlation analysis and the WEKA attribute function were used as input neurons for the construction of neural network (NN) models, in the Fast Artificial Neural Network Tool 1.2 (FANN Tool 1.2). The optimum NN model resulted in a mean squared error (MSE) of 8.99899 × 10−5 and was based on treatment capacity, flow rate, influent load, and served population as its inputs. Notably, the research highlights the potential generalizability of these models in Greece and beyond the Greek context, offering valuable tools for stakeholders to inform decision making, allocate resources efficiently, and improve energy-efficient designs, resulting in cost savings and sustainability benefits.
Projected Reductions in CO2 Emissions by Using Alternative Methanol Fuel to Power a Service Operation Vessel
Authors: Bortnowska Monika
Published: November 2023

Due to increasingly stringent IMO and European Commission requirements for greenhouse gas emissions, the present study analysed the projected reductions in CO2 emissions achieved by using methanol as an alternative fuel to power custom service operation vessels (SOVs) serving wind platforms in the Baltic Sea. Methanol is a relatively new fuel, approved for use as a safe marine fuel in the late 2020s. In these analyses, reference was made to the current interim guidelines, supplementing the IGF Code in the form of MSC.1/Circ.1621. The SOV type was chosen because of the current growing demand for these ships (the dynamic development of offshore wind power) and the lack of analyses of this type of small craft. The importance of assessing CO2 emissions in this case is due to the specifics of the vessel’s operation in different modes, and thus the variable load on the propulsion system and the area of operation close to the coastline. A computational research method was used to evaluate CO2 emissions, as well as the cost of methanol fuel, using current regulations and technical data. A comparison was also made between conventional MDO and LNG fuels. The first results of the analysis showed that methanol fuel is only competitive with MDO (a few-percent advantage) in terms of the average estimated index value EIV. Economically, it will require a higher investment, despite the favourable unit price of methanol compared to LNG and MDO.
Smart Energy Borrowing and Relaying in Wireless-Powered Networks: A Deep Reinforcement Learning Approach
Authors: Mondal Abhishek , Alam Md. Sarfraz , Mishra Deepak , Prasad Ganesh
Published: November 2023

Wireless energy harvesting (EH) communication has long been considered a sustainable networking solution. However, it has been limited in efficiency, which has been a major obstacle. Recently, strategies such as energy relaying and borrowing have been explored to overcome these difficulties and provide long-range wireless sensor connectivity. In this article, we examine the reliability of a wireless-powered communication network by maximizing the net bit rate. To accomplish our goal, we focus on enhancing the performance of hybrid access points and information sources by optimizing their transmit power. Additionally, we aim to maximize the use of harvested energy, by using energy-harvesting relays for both information transmission and energy relaying. However, this optimization problem is complex, as it involves non-convex variables and requires combinatorial relay selection indicator optimization for decode and forward (DF) relaying. To simplify this problem, we utilize the Markov decision process and deep reinforcement learning framework based on the deep deterministic policy gradient algorithm. This approach enables us to tackle this non-tractable problem, which conventional convex optimization techniques would have difficulty solving in complex problem environments. The proposed algorithm significantly improved the end-to-end net bit rate of the smart energy borrowing and relaying EH system by 13.22%, 27.57%, and 14.12% compared to the benchmark algorithm based on borrowing energy with an adaptive reward for Quadrature Phase Shift Keying, 8-PSK, and 16-Quadrature amplitude modulation schemes, respectively.
Analysis of the Use of a Low-Power Photovoltaic System to Power a Water Pumping Station in a Tourist Town
Authors: Świętochowski Kamil , Świętochowska Martyna , Kalenik Marek , Gwoździej-Mazur Joanna
Published: November 2023

The increase in electricity generation prices represents a reason why water utility companies are looking for ways to reduce costs. One of the first ideas of users was to build photovoltaic installations. Water treatment plants or sewage treatment plants usually have large unused areas. They look different in facilities that consume a lot of energy but occupy little land, and include water intakes (wells) and water pumping stations. Facilities equipped with pumps are characterized by high electricity consumption. This article assesses the possibility of using PV installations at the water intake. An analysis of energy production from the 3.0 kW PV installation in Polanica-Zdrój was carried out, and then, simulations of the possibility of providing energy via installations with capacities of 3.0 kW, 4.2 kW, and 6.0 kW were performed. Analyses of energy production and demand, as well as analyses of water production based on annual, monthly, daily, and hourly data, were performed. An analysis of the hourly coverage of the WPS’s demand for electricity was carried out with regard to the current production of energy from the PV installation, as was an analysis of the overproduction of energy from the PV installation regarding the energy demand of the WPS. The simulation results are presented for cloudy and sunny days.
Characterization Specifications for FRP Pultruded Materials: From Constituents to Pultruded Profiles
Authors: Harati Khalilabad Ehsan , Ruiz Emparanza Alvaro , De Caso Francisco , Roghani Hossein , Khodadadi Nima , Nanni Antonio
Published: November 2023

Pultruded FRP composites have emerged as a promising alternative to traditional materials like concrete, steel, and timber, especially in corrosive environmental conditions. However, the unique properties of these composites necessitate careful consideration during their implementation, as they differ significantly from conventional materials. Proper testing and characterization of FRP pultruded materials is key for their efficient and safe implementation. However, the existing specifications are not unified, resulting in ambiguity among stakeholders. This paper aims to bridge this gap by thoroughly reviewing current destructive and non-destructive test methods for FRP pultruded materials, specifications, quality control, and health monitoring of FRP structures. Each subsection is further divided into subtopics, providing a comprehensive overview of the subject. By shedding light on these crucial aspects, this article aims to accelerate the adoption and utilization of these innovative materials in practical applications.
Thermal Characteristics of Epoxy Fire-Retardant Coatings under Different Fire Regimes
Authors: Gravit Marina , Shabunina Daria , Shcheglov Nikita
Published: November 2023

Different systems of fire protection coatings are used to protect the metal structures of stories and trestles at oil and gas facilities from low (when filling cryogenic liquids) and high temperatures (in case of the possible development of a hydrocarbon fire regime). This paper presents the results of experiments of fireproof coatings on an epoxy binder after the simulation of a liquefied hydrocarbons spill and subsequent development of a hydrocarbon fire regime at the object of protection and exposure of structures to a standard fire regime. According to the experimental results, the temperatures on the samples at the end of the cryogenic exposure were determined and the time from the beginning of the thermal exposure to the limit state of the samples at a hydrocarbon and standard temperature fire regime was determined. As a result, temperature–time curves in the hydrocarbon and standard fire regimes were obtained, showing good convergence with the simulation results. The solution of the inverse task of heat conduction using finite element modeling made it possible to determine the thermophysical properties of the formed foam coke at the end of the fire tests of steel structures with intumescent coatings. It was determined that an average of 12 mm of intumescent coating thickness is required to achieve a fire protection efficiency of 120 min and for the expected impact of the hydrocarbon fire regime, the coating consumption should be increased by 1.5–2 times compared to the coating consumption for the standard regime.
In Vitro Fermentation Characteristics of Fungal Polysaccharides Derived from Wolfiporia cocos and Their Effect on Human Fecal Microbiota
Authors: Ma Ka Lee , Kei Nelson , Yang Fan , Lauw Susana , Chan Po Lam , Chen Lei , Cheung Peter Chi Keung
Published: November 2023

Gut microbiota has been described as a new ‘organ’ that interferes with host physiology by its metabolites produced from the utilization and biotransformation of undigested food components. Fu Ling (FL), the sclerotia of fungi Wolfiporia cocos, contains β-glucan, which is a known natural polysaccharide with strong medicinal efficacy. This study endeavors to evaluate the fermentability of FL and polysaccharides extracted from its sclerotia. An in vitro fermentation of structurally characterized FL and its β-glucan by human fecal microbiota was conducted. Total bacterial count, pH change, short-chain fatty acid profile and microbiota profile were assessed post-fermentation. FL containing over 70% of β-(1 → 3) and (1 → 6)-glucans with a low degree of branching of 0.24 could enhance acetic acid (a major microbial metabolite) production. Both FL and its extracted β-glucan had similar modulation on microbial composition. They enriched Phascolarctobacterium faecium, Bacteroides dorei and Parabacteroides distasonis, all of which are shown to possess anti-inflammatory effects. FL polysaccharide can be utilized as a natural whole food for its potential health benefits to human gut bacteria.
Dynamic Thermal Treatments in Green Coconut Water Induce Dynamic Stress Adaptation of Listeria innocua That Increases Its Thermal Resistance
Authors: González-Tejedor Gerardo A. , Garre Alberto , Iguaz Asunción , Wong-Zhang Ricardo , Fernández Pablo S. , Possas Arícia
Published: November 2023

The global coconut water market is projected to grow in the upcoming years, attributed to its numerous health benefits. However, due to its susceptibility to microbial contamination and the limitations of non-thermal decontamination methods, thermal treatments remain the primary approach to ensure the shelf-life stability and the microbiological safety of the product. In this study, the thermal inactivation of Listeria innocua, a Listeria monocytogenes surrogate, was evaluated in coconut water and in tryptone soy broth (TSB) under both isothermal (50–60 °C) and dynamic conditions (from 30 to 60 °C, with temperature increases of 0.5, 1 and 5 °C/min). Mathematical models were used to analyse the inactivation data. The Geeraerd model effectively described the thermal inactivation of L. innocua in both TSB and coconut water under isothermal conditions, with close agreement between experimental data and model fits. Parameter estimates and analysis revealed that acidified TSB is a suitable surrogate medium for studying the thermal inactivation of L. innocua in coconut water, despite minor differences observed in the shoulder length of inactivation curves, likely attributed to the media composition. The models fitted to the data obtained at isothermal conditions fail to predict L. innocua responses under dynamic conditions. This is attributed to the stress acclimation phenomenon that takes place under dynamic conditions, where bacterial cells adapt to initial sub-lethal treatment stages, leading to increased thermal resistance. Fitting the Bigelow model directly to dynamic data with fixed z-values reveals a three-fold increase in D-values with lower heating rates, supporting the role of stress acclimation. The findings of this study aid in designing pasteurization treatments targeting L. innocua in coconut water and enable the establishment of safe, mild heat treatments for refrigerated, high-quality coconut water.
Advances in Designing Essential Oil Nanoformulations: An Integrative Approach to Mathematical Modeling with Potential Application in Food Preservation
Authors: Soni Monisha , Yadav Arati , Maurya Akash , Das Somenath , Dubey Nawal Kishore , Dwivedy Abhishek Kumar
Published: November 2023

Preservation of foods, along with health and safety issues, is a growing concern in the current generation. Essential oils have emerged as a natural means for the long-term protection of foods along with the maintenance of their qualities. Direct applications of essential oils have posed various constraints to the food system and also have limitations in application; hence, encapsulation of essential oils into biopolymers has been recognized as a cutting-edge technology to overcome these challenges. This article presents and evaluates the strategies for the development of encapsulated essential oils on the basis of fascination with the modeling and shuffling of various biopolymers, surfactants, and co-surfactants, along with the utilization of different fabrication processes. Artificial intelligence and machine learning have enabled the preparation of different nanoemulsion formulations, synthesis strategies, stability, and release kinetics of essential oils or their bioactive components from nanoemulsions with improved efficacy in food systems. Different mathematical models for the stability and delivery kinetics of essential oils in food systems have also been discussed. The article also explains the advanced application of modeling-based encapsulation strategies on the preservation of a variety of food commodities with their intended implication in food and agricultural industries.
Characterization of the Toxigenic Potential of Bacillus cereus sensu lato Isolated from Raw Berries and Their Products
Authors: Oliveira Márcia , Carvalho Marta , Teixeira Paula
Published: November 2023

Bacillus cereus is estimated to be responsible for 1.4–12% of all food poisoning outbreaks worldwide. The objective of this study was to investigate the toxigenic potential of 181 isolates of B. cereus previously recovered from different types of berries and berry products (strawberries, raspberries, blackberries, and blueberries) by assessing the presence of enterotoxin genes (hblA, hblC, hblD, nheA, nheB, nheC, and cytK) and an emetic toxin cereulide synthetase gene (ces). The cytotoxic activity on Caco-2 cells was also evaluated for the two isolates containing the gene cytK. Twenty-three toxigenic profiles were found. The nheABC (91.7%) and hblACD (89.0%) complexes were the most prevalent among the isolates, while the cytK and ces genes were detected in low percentages, 1.1% and 3.3%, respectively. In addition, the nheABC/hblACD complex and ces genes were detected in isolates recovered throughout the production process of blackberries and strawberries. The cytotoxic activity on Caco-2 cells was also observed to be greater than 60% for isolates containing the cytK gene.
Heat Extraction in Geothermal Systems with Variable Thermo-Poroelastic Fracture Apertures
Authors: Singh Mrityunjay , Mahmoodpour Saeed , Bär Kristian , Sass Ingo
Published: November 2023

The fracture network largely determines the efficiency of heat extraction from fractured geothermal reservoirs. Fracture openings are influenced by thermo-poroelastic stresses during cold fluid flow, with the interplay between fracture length and fracture opening regulating heat transfer. The lack of field data concerning fluctuating fracture openings underscores the necessity for computational models. This work emphasizes the impact of such gaps in the literature. Factors such as temperature, pressure, stress, thermal breakthrough time, and cumulative energy are evaluated to analyze the system’s behavior. A sensitivity analysis is employed to ascertain the significance of stress on fracture opening, compared with thermo-hydraulic behavior. The results show that stress field alterations, due to intersections with minor fractures, can cause up to a 15% variation in the largest fracture’s opening. The impact of thermoelastic stress outweighs the impact of poroelastic stress approximately threefold. Such stress-induced variations in fracture openings can lead to an up to 30% increase in cumulative heat extraction, while the drop in production temperature is limited to around 50%.
Get In and Get Out: White Racial Transformation and the US Gothic Imagination
Authors: Murray Hannah Lauren
Published: November 2023

This article examines the Gothic trope of White racial transformation in Robert Montgomery Bird’s Sheppard Lee (1836) and Jordan Peele’s Get Out (2017). These seemingly disparate texts both feature White men who turn Black via supernatural body hopping or experimental surgery. In these texts, Blackness acts as an emotional and material resource for White characters that perversely bolsters Whiteness by escaping it. Little-known outside of antebellum specialisms, Sheppard Lee enhances our understanding of race in the Gothic by considering why Whiteness may be rejected in the early nation. Written in the context of blackface minstrelsy, the novel transforms downwardly mobile Sheppard into an enslaved man as a respite from the pressures of economic success. Get Out builds on its nineteenth-century precursors by showing the Black body as a desired and necessary vessel for the “post-racial” White American self, who swaps their physical Whiteness for Blackness to extend or enhance their own life, turning Black men into extensions and enforcers of White middle-class culture. In uniting these texts through the lens of critical Whiteness studies, this article argues that White racial transformation is a long-held tradition in the US Gothic that not only expresses White desires and anxieties, but itself transforms in each specific historical racial context.
The Use of Prehospital Intensive Care Units in Emergencies—A Scoping Review
Authors: Alruwaili Abdullah , Khorram-Manesh Amir , Ratnayake Amila , Robinson Yohan , Goniewicz Krzysztof
Published: November 2023

Background: Amidst a rising tide of trauma-related emergencies, emergency departments worldwide grapple with the challenges of overcrowding and prolonged patient wait times. Addressing these challenges, the integration of prehospital intensive care units has appeared as a promising solution, streamlining trauma care and enhancing patient safety. Nevertheless, the feasibility of such an initiative becomes murky when considered globally. This review delves into the intricacies of prehospital intensive care units’ deployment for trauma care, scrutinizing their configurations, operational practices, and the inherent challenges and research priorities. Methods: A scoping review was performed for eligible studies. The result was uploaded to the RAYYAN research platform, facilitating simultaneous evaluation of the studies by all researchers. Results: A total of 42 studies were initially selected. Four studies were duplicates, and 25 studies were unanimously removed as irrelevant. The remaining studies (n = 13) were included in the review, and the outcomes were categorized into diverse subgroups. Conclusions: A country’s emergency medical services must achieve specific milestones in education, competency, resource availability, and performance to effectively harness the potential of a prehospital intensive care unit. While certain nations are equipped, others lag, highlighting a global disparity in readiness for such advanced care modalities.
The Impact of Resilience on Post-Traumatic Growth among Nurses in COVID-19-Designated Hospitals: The Mediating Effect of Meaning in Life
Authors: Han Suk-Jung , Yeun Young-Ran , Roh Hyunseung
Published: November 2023

This study aimed to confirm the relationship between resilience, meaning in life, and post-traumatic growth (PTG) among nurses during the COVID-19 pandemic. In particular, the mediating effect of meaning in life on the relationship between resilience and PTG was examined. A cross-sectional descriptive research design was used. The participants were 220 nurses at COVID-19-designated hospitals in Seoul. Descriptive statistics, a t-test, ANOVA, Pearson’s correlation coefficient analysis, hierarchical regression, and SPSS PROCESS macro (Model 4) were used for data analyses. As a result of the study, resilience and meaning in life each had a significant positive effect on PTG. In addition, the indirect effect of meaning in life was significant, suggesting that meaning in life partially mediated the relationship between resilience and PTG. The results indicate that nurses’ resilience directly contributes to the improvement of PTG, and this relationship is further strengthened indirectly through the presence of meaning in life. Therefore, it is suggested that strategies should be developed to enhance resilience and promote a sense of meaning in the profession in order to support the mental health and foster growth among nurses.
Technologies for Managing the Health of Older Adults with Multiple Chronic Conditions: A Systematic Literature Review
Authors: Cajamarca Gabriela , Proust Valentina , Herskovic Valeria , Cádiz Rodrigo F. , Verdezoto Nervo , Fernández Francisco J.
Published: November 2023

Multimorbidity is defined as the presence of two or more chronic medical conditions in a person, whether physical, mental or long-term infectious diseases. This is especially common in older populations, affecting their quality of life and emotionally impacting their caregivers and family. Technology can allow for monitoring, managing, and motivating older adults in their self-care, as well as supporting their caregivers. However, when several conditions are present at once, it may be necessary to manage several types of technologies, or for technology to manage the interaction between conditions. This work aims to understand and describe the technologies that are used to support the management of multimorbidity for older adults. We conducted a systematic review of ten years of scientific literature from four online databases. We reviewed a corpus of 681 research papers, finally including 25 in our review. The technologies used most frequently by older adults with multimorbidity are mobile applications and websites, and they are mostly focused on communication and connectivity. We then propose opportunities for future research on addressing the challenges in the management of several simultaneous health conditions, potentially creating a better approach than managing each condition as if it were independent.
Differences in Motor Imagery Ability between People with Parkinson’s Disease and Healthy Controls, and Its Relationship with Functionality, Independence and Quality of Life
Authors: Ferreira-Sánchez María , Moreno-Verdú Marcos , Atín-Arratibel María de los Ángeles , Martín-Casas Patricia
Published: November 2023

Motor imagery (MI) has been shown to be effective for the acquisition of motor skills; however, it is still unknown whether similar benefits can be achieved in neurological patients. Previous findings of differences in MI ability between people with Parkinson’s disease (PwPD) and healthy controls (HCs) are mixed. This study examined differences in the ability to both create and maintain MI as well as investigating the relationship between the ability to create and maintain MI and motor function, independence and quality of life (QoL). A case–control study was conducted (31 PwPD and 31 HCs), collecting gender, age, dominance, socio-demographic data, duration and impact of the disease. MI intensity (MIQ-RS and KVIQ-34) and temporal accuracy of MI (imagined box and block test [iBBT], imagined timed stand and walk test [iTUG]) were assessed. Functional and clinical assessments included upper limb motor function, balance, gait, independence in activities of daily living and quality of life measures. Statistically significant differences in temporal accuracy were observed and partial and weak relationships were revealed between MI measures and functioning, independence and QoL. PwPD retain the ability to create MI, indicating the suitability of MI in this population. Temporal accuracy might be altered as a reflection of bradykinesia on the mentally simulated actions.
Optimization and Simulation of Mountain City Land Use Based on MOP-PLUS Model: A Case Study of Caijia Cluster, Chongqing
Authors: Zhong Yuqing , Zhang Xiaoxiang , Yang Yanfei , Xue Minghui
Published: November 2023

Mountainous cities face various land use challenges, including complex topography, low land use efficiency, and the insufficient control of land use in small-scale areas at the urban fringe. Considering population changes, environmental conservation, and urban planning, this study first established three scenarios: economic priority (Econ. Prior.), ecological priority (Ecol. Prior.), and balanced development (BD), and then used the Multi-Objective Planning (MOP) model to calculate the optimal land use structure. Finally, it carried out land use spatial layout optimization based on the Patch-generating Land Use Simulation (PLUS) model in 2035, Caijia Cluster, Chongqing, China. This approach, known as MOP-PLUS modeling, aimed to optimize land use. Meanwhile, the applicability of the PLUS model in simulating land use changes was discussed in small-scale mountainous areas. The results show the following: (1) The “quantity + space” approach in the MOP-PLUS model demonstrated the feasibility of the PLUS model in simulating land use change in small-scale mountainous areas. The overall accuracy (OA) of land use change simulation reached 81.60%, with a Kappa value of 0.73 and a Figure of Merit (FoM) coefficient of 0.263. (2) Land use optimization: Under the Econ. Prior. scenario, economic benefits peaked at 4.06 × 1010 CNY. Urban expansion was the largest, leading to increased patch fragmentation. The Ecol. Prior. scenario yielded the highest ecological benefits, reaching 7.46 × 107 CNY. The urban development pattern exhibited inward contraction, accompanied by urban retrogression. In the BD scenario, economic benefits totaled 3.89 × 1010 CNY, and ecological benefits amounted to 7.16 × 107 CNY. Construction land tended to concentrate spatially, leading to relatively optimal land use efficiency. Therefore, based on a comprehensive consideration of the regional land use constraint policies and spatial layout, we believe that a balance point for land use demands can be found in the BD scenario. It can ensure economic growth without compromising the ecological environment.
Cucurbitacin I Reverses Tumor-Associated Macrophage Polarization to Affect Cancer Cell Metastasis
Authors: Gong Xiaocheng , Liu Yunfei , Liang Keying , Chen Zixi , Ding Ke , Qiu Li , Wei Jinfen , Du Hongli
Published: November 2023

The tumor microenvironment plays a critical role in tumor progression and immune regulation. As one of the most important components of the tumor microenvironment, macrophages have become a new therapeutic target for inhibiting tumor progression. Despite the well-documented anticancer activity of cucurbitacin I, its effect on macrophages remains unclear. In this study, we established a coculture system of macrophages and cancer cells under hypoxic conditions to simulate the tumor-promoting environment mediated by M2-like macrophages. We determined whether cucurbitacin I modulates M2-like polarization in macrophages in vitro and conducted RNA sequencing to identify gene expression changes induced by cucurbitacin I in macrophages. The results indicated a remarkable inhibition of the M2-like polarization phenotype in macrophages following treatment with cucurbitacin I, which was accompanied by the significant downregulation of heme oxygenase-1. Moreover, we found that cucurbitacin I-treated macrophages reduced the migration of cancer cells by inhibiting the M2 polarization in vitro. These findings highlight the potential of cucurbitacin I as a therapeutic agent that targets M2-like macrophages to inhibit cancer cell metastasis. Our study provides novel insights into the intricate interplay among macrophage polarization, cucurbitacin I, and heme oxygenase-1, thereby opening new avenues for cancer treatment.
Modeling Liver Development and Disease in a Dish
Authors: Iqbal Waqas , Wang Yaru , Sun Pingnan , Zhou Xiaoling
Published: November 2023

Historically, biological research has relied primarily on animal models. While this led to the understanding of numerous human biological processes, inherent species-specific differences make it difficult to answer certain liver-related developmental and disease-specific questions. The advent of 3D organoid models that are either derived from pluripotent stem cells or generated from healthy or diseased tissue-derived stem cells have made it possible to recapitulate the biological aspects of human organs. Organoid technology has been instrumental in understanding the disease mechanism and complements animal models. This review underscores the advances in organoid technology and specifically how liver organoids are used to better understand human-specific biological processes in development and disease. We also discuss advances made in the application of organoid models in drug screening and personalized medicine.
The Effect of Anti-Autotaxin Aptamers on the Development of Proliferative Vitreoretinopathy
Authors: Hanazaki Hirotsugu , Yokota Harumasa , Yamagami Satoru , Nakamura Yoshikazu , Nagaoka Taiji
Published: November 2023

This study investigated the effect of anti-autotaxin (ATX) aptamers on the development of proliferative vitreoretinopathy (PVR) in both in vivo and in vitro PVR swine models. For the in vitro study, primary retinal pigment epithelial (RPE) cells were obtained from porcine eyes and cultured for cell proliferation and migration assays. For the in vivo study, a swine PVR model was established by inducing retinal detachment and injecting cultured RPE cells (2.0 × 106). Concurrently, 1 week after RPE cell injection, the anti-ATX aptamer, RBM-006 (10 mg/mL, 0.1 mL), was injected twice into the vitreous cavity. Post-injection effects of the anti-ATX aptamer on PVR development in the in vivo swine PVR model were investigated. For the in vitro evaluation, the cultured RPE cell proliferation and migration were significantly reduced at anti-ATX aptamer concentrations of 0.5–0.05 mg and at only 0.5 mg, respectively. Intravitreal administration of the anti-ATX aptamer also prevented tractional retinal detachment caused by PVR in the in vivo PVR model. We observed that the anti-ATX aptamer, RBM-006, inhibited PVR-related RPE cell proliferation and migration in vitro and inhibited the progression of PVR in the in vivo model, suggesting that the anti-ATX aptamer may be effective in preventing PVR.
Network Pharmacology Combined with Machine Learning to Reveal the Action Mechanism of Licochalcone Intervention in Liver Cancer
Authors: Guo Fangfang , Yang Xiaotang , Hu Chengxiang , Li Wannan , Han Weiwei
Published: November 2023

There are reports indicating that licochalcones can inhibit the proliferation, migration, and invasion of cancer cells by promoting the expression of autophagy-related proteins, inhibiting the expression of cell cycle proteins and angiogenic factors, and regulating autophagy and apoptosis. This study aims to reveal the potential mechanisms of licochalcone A (LCA), licochalcone B (LCB), licochalcone C (LCC), licochalcone D (LCD), licochalcone E (LCE), licochalcone F (LCF), and licochalcone G (LCG) inhibition in liver cancer through computer-aided screening strategies. By using machine learning clustering analysis to search for other structurally similar components in licorice, quantitative calculations were conducted to collect the structural commonalities of these components related to liver cancer and to identify key residues involved in the interactions between small molecules and key target proteins. Our research results show that the seven licochalcones molecules interfere with the cancer signaling pathway via the NF-κB signaling pathway, PDL1 expression and PD1 checkpoint pathway in cancer, and others. Glypallichalcone, Echinatin, and 3,4,3′,4′-Tetrahydroxy-2-methoxychalcone in licorice also have similar structures to the seven licochalcones, which may indicate their similar effects. We also identified the key residues (including ASN364, GLY365, TRP366, and TYR485) involved in the interactions between ten flavonoids and the key target protein (nitric oxide synthase 2). In summary, we provide valuable insights into the molecular mechanisms of the anticancer effects of licorice flavonoids, providing new ideas for the design of small molecules for liver cancer drugs.
The Reactions of Alkenes with Phenyl-N-triflylimino-λ3-iodane: Solvent and Oxidant Impact
Authors: Moskalik Mikhail Yu. , Ganin Anton S. , Shainyan Bagrat A.
Published: November 2023

The reactions of alkenes with phenyl-N-triflylimino-λ3-iodane PhI=NTf (1) have been studied in different conditions. In methylene chloride, in the presence of N-halosuccinimides, the products of mono and bis-triflamidation were obtained. In MeCN, the product of bromotriflamidation (with NBS) with solvent interception or of bis-triflamidation (with NIS) is formed. The reaction with trans-stilbene in acetonitrile with NBS gave rise to cyclization to 2-methyl-4,5-diphenyl-1-triflyl-4,5-dihydro-1H-imidazole. In contrast, with NIS as an oxidant, both in CH2Cl2 and MeCN, the major product was 2,3-diphenyl-1-triflylaziridine formed in good yield. With NBS, aziridine is also formed but as a minor product, the major one being a mixture of diastereomers of the product of bromotriflamidation. The reaction of compound 1 with vinylcyclohexane in methylene chloride affords the mixtures of regioisomers of the products of halotriflamidation, whereas in acetonitrile, the products of solvent interception and cyclization to the imidazoline are formed. A mechanism explaining the formation of all isolated products is proposed.
Toward a Transportable Cell Culture Platform for Evaluating Radiotherapy Dose Modifying Factors
Authors: Carlson Nicholas , House Carrie D. , Tambasco Mauro
Published: November 2023

The current tools for validating dose delivery and optimizing new radiotherapy technologies in radiation therapy do not account for important dose modifying factors (DMFs), such as variations in cellular repair capability, tumor oxygenation, ultra-high dose rates and the type of ionizing radiation used. These factors play a crucial role in tumor control and normal tissue complications. To address this need, we explored the feasibility of developing a transportable cell culture platform (TCCP) to assess the relative biological effectiveness (RBE) of ionizing radiation. We measured cell recovery, clonogenic viability and metabolic viability of MDA-MB-231 cells over several days at room temperature in a range of concentrations of fetal bovine serum (FBS) in medium-supplemented gelatin, under both normoxic and hypoxic oxygen environments. Additionally, we measured the clonogenic viability of the cells to characterize how the duration of the TCCP at room temperature affected their radiosensitivity at doses up to 16 Gy. We found that (78±2)% of MDA-MB-231 cells were successfully recovered after being kept at room temperature for three days in 50% FBS in medium-supplemented gelatin at hypoxia (0.4±0.1)% pO2, while metabolic and clonogenic viabilities as measured by ATP luminescence and colony formation were found to be (58±5)% and (57±4)%, respectively. Additionally, irradiating a TCCP under normoxic and hypoxic conditions yielded a clonogenic oxygen enhancement ratio (OER) of 1.4±0.6 and a metabolic OER of 1.9±0.4. Our results demonstrate that the TCCP can be used to assess the RBE of a DMF and provides a feasible platform for assessing DMFs in radiation therapy applications.
Sperm Chromatin Status and DNA Fragmentation in Mouse Species with Divergent Mating Systems
Authors: Agudo-Rios Clara , Sanchez-Rodriguez Ana , Idrovo Ingrid I. D. , Laborda-Gomariz Juan Ángel , Soler Ana J. , Teves Maria E. , Roldan Eduardo R. S.
Published: November 2023

Sperm DNA integrity and chromatin status serve as pivotal indicators of sperm quality, given their intricate link to sperm function, embryo development, and overall fertility. Defects in chromatin compaction, which are often associated with compromised protamine content, can lead to damaged DNA strands. In this study, the chromatin status and possible correlation with DNA damage was assessed in males of three mouse species: Mus musculus, M. spretus, and M. spicilegus. We employed various staining methods, including aniline blue, methylene blue (Diff-Quik), toluidine blue, and chromomycin A3, to assess chromatin compaction in cauda epididymal sperm. Samples were also analyzed by the sperm chromatin structure assay (SCSA) to estimate DNA fragmentation (%tDFI, %HDS). Analyses were carried out on freshly collected sperm and cells incubated for 3 h in a HEPES-buffered modified Tyrode’s medium simulating conditions of the female reproductive tract. Notably, the analysis of chromatin status yielded minimal abnormal values across all three species employing diverse methodologies. SCSA analyses revealed distinct variations in %tDFI between species. Following sperm incubation, the percentages of sperm stained with methylene blue exhibited differences among the species and were significantly correlated to the DNA fragmentation index. HDS demonstrated correlations with the percentages of sperm stained by aniline blue, methylene blue, and chromomycin A3. Overall, chromatin compaction was high across all species, with limited differences among them. The relationship between chromatin status and DNA integrity appeared to be related to levels of sperm competition among species.
Temporal Convolutional Networks and BERT-Based Multi-Label Emotion Analysis for Financial Forecasting
Authors: Liapis Charalampos M. , Kotsiantis Sotiris
Published: November 2023

The use of deep learning in conjunction with models that extract emotion-related information from texts to predict financial time series is based on the assumption that what is said about a stock is correlated with the way that stock fluctuates. Given the above, in this work, a multivariate forecasting methodology incorporating temporal convolutional networks in combination with a BERT-based multi-label emotion classification procedure and correlation feature selection is proposed. The results from an extensive set of experiments, which included predictions of three different time frames and various multivariate ensemble schemes that capture 28 different types of emotion-relative information, are presented. It is shown that the proposed methodology exhibits universal predominance regarding aggregate performance over six different metrics, outperforming all the compared schemes, including a multitude of individual and ensemble methods, both in terms of aggregate average scores and Friedman rankings. Moreover, the results strongly indicate that the use of emotion-related features has beneficial effects on the derived forecasts.
Impact of Pemafibrate Therapy on Reducing Small Dense Low-Density-Lipoprotein-Cholesterol Levels in Patients with Hypertriglyceridemia
Authors: Hida Yuki , Imamura Teruhiko , Kinugawa Koichiro
Published: November 2023

Background: Small dense LDL-cholesterol is a recently discovered cardiovascular risk factor beyond LDL-cholesterol. Pemafibrate is a novel selective peroxisome proliferator-activated receptor-α modulator that reduces triglyceride levels. Given the significant association between triglycerides and small dense LDL-cholesterol levels, pemafibrate may reduce the levels of small dense LDL-cholesterol. Methods: Patients with hypertriglyceridemia who started pemafibrate therapy and continued it for >3 months between 2018 and 2022 were included in this retrospective study. The levels of small dense LDL-cholesterol, which was estimated using Sampson’s equation, consisting of the LDL-cholesterol and triglyceride levels, were compared between baseline and 3-month follow-up. Results: A total of 98 patients receiving pemafibrate therapy (median age: 63 years, 69 male) were eligible, including 33 patients (34%) who received concomitant statins. Small dense LDL-cholesterol levels decreased significantly during the course of 3-month pemafibrate therapy from 48.9 (IQR: 35.7, 57.9) mg/dL to 38.8 (IQR: 30.0, 45.1) mg/dL, regardless of the concomitant administration of statins (p < 0.001). The rate of cardiovascular events decreased significantly from the pre-treatment 1-year period to the treatment 1-year period (from 13 to 2 events, from 0.133 to 0.021 events per year, incidence rate ratio: 0.16, 95% confidence interval: 0.14–0.17, p < 0.001). Conclusions: Pemafibrate therapy may mitigate the concentrations of small dense LDL-cholesterol autonomously in patients manifesting hypertriglyceridemia within the authentic clinical milieu. The clinical importance of the diminishment in small dense LDL-cholesterol instigated via pemafibrate merits further scrutiny.