bluuebunny/crossref_metadata_2025 huggingface dataset:
Brief research report: Investigating neurodiversity acceptance in the college classroom: an exploratory study
Authors: Hotez Emily , Rava Julianna , Kuo Alice A.
Published: August 2023

Introduction

Higher education represents a potential opportunity to address autism stigma and, in turn, prevent the negative health consequences of stigma as autistic college students transition into adulthood. The purpose of the current study was to investigate the general college student body and describe their (1) level of autism acceptance based on the neurodiversity framework utilizing the Autism Attitude Acceptance Scale (AAAS) and (2) self-reported knowledge and interests about neurodiversity.

Methods

The sample (n = 90, M age = 21.86, SD = 1.18) featured undergraduates enrolled in a large, diverse, public university in the U.S. Approximately 32% (n = 29) identified as neurodivergent, with 5 identifying as autistic. Separate groups of students completed the AAAS—administered via Qualtrics—in February and September of 2022. In addition, students were provided the opportunity to ask up to three questions each about neurodiversity. We utilized qualitative thematic coding to extract themes from the neurodiversity questions.

Results

We derived frequency and descriptive statistics for each item and the two AAAS subscales: (1) the General Acceptance (GA) subscale (Cronbach’s alpha = 0.89) and (2) the Attitudes toward Treating Autistic Behavior (ATAB) subscale (Cronbach’s alpha = 0.77). Responses reflected high general autism acceptance. Responses demonstrated greater variability, however, with respect to attitudes toward treating autistic behaviors. Qualitative findings revealed both a high degree of interest in the neurodiversity movement, as well as high variability in knowledge and familiarity with its tenets.

Conclusion

This research can inform higher education’s efforts to address and prevent stigma towards autistic and other neurodivergent students.
User experiences during the transition to calibration-free sensors with remote monitoring while using automated insulin delivery - a qualitative study
Authors: Sehgal Shekhar , De Bock Martin , Jones Shirley , Frewen Carla , Wheeler Benjamin J.
Published: August 2023

Introduction

To evaluate the experiences of patients with type 1 diabetes following transition from a calibration-requiring to a calibration-free sensor and remote monitoring in the context of using automated insulin delivery (AID).

Research design and methods

Fifteen participants aged 7–65 years with type 1 diabetes participating in a longitudinal study used a Medtronic® advanced hybrid closed loop (AHCL) device with initially calibration-requiring then calibration-free sensors. Qualitative interviews were conducted ≥20 weeks following use of the calibration-requiring and ≥4 weeks after use of the calibration-free sensors/remote monitoring. Thematic analysis was used to identify key themes and subthemes.

Results

At baseline, mean diabetes duration was 14.5 years ( ± 10.9), mean Hba1c 54.8 mmol/mol ( ± 10.2) (7.2 ± 0.9%) and Time in range 75.4% ( ± 11.6). Participants reported a progressive improvement in digital and lifestyle integration, and device trust following transition to calibration-free sensors with remote monitoring potential. They also reported a reduced need for capillary glucose, increased device satisfaction and trust, and reduced burden of diabetes care. Negative aspects reported included periodic early sensor loss, and for some, impaired integration with mobile devices.

Conclusion

Transitioning to calibration-free sensors with remote monitoring while using AHCL was associated with better user experience, including perceptions of improved quality of life and a reduced burden of diabetes care. Appropriate expectation setting, training, and ongoing support allow for the optimal user experience while using AHCL.

Clinical trial registration

https://www.anzctr.org.au, identifier ACTRN12621000360819
Circulating bile acid profile characteristics in PCOS patients and the role of bile acids in predicting the pathogenesis of PCOS
Authors: Yu Jie , Zhang Yi , Zhu Yuchen , Li Yushan , Lin Siyu , Liu Wei , Tao Tao
Published: August 2023

Background

The metabolic profile of bile acids and their potential role as biomarkers in the pathogenesis of polycystic ovary syndrome (PCOS) have not been thoroughly characterized. Assessing their predictive value for PCOS is of significant importance.

Methods

In this study, we enrolled 408 women with PCOS and 204 non-PCOS controls. The serum bile acid profile was measured using high-performance liquid chromatography-tandem mass spectrometry (LC/MS). We analyzed the differences in serum bile acid profiles between PCOS patients using the OPLS-DA model. Additionally, we examined the relationship between bile acid profiles and parameters related to glucose metabolism and hyperandrogenism. ROC analysis was employed to identify potential biomarkers for PCOS pathogenesis. XGboost was utilized for cross-validation.

Results

The bile acid profile was found to be altered in PCOS patients. Specifically, the primary and secondary unconjugated bile acid fractions were significantly higher in the PCOS population. We identified five bile acid metabolite candidates that exhibited the most significant differences between PCOS and non-PCOS controls. DCA was associated with deposition index, fasting and postprandial insulin but was influenced by testosterone. CDCA and LCA combined with testosterone showed potential as biomarkers for the pathogenesis of PCOS.

Conclusion

The circulating bile acid profile undergoes changes in PCOS. DCA is associated with deposition index, fasting and postprandial insulin and its level is influenced by testosterone. CDCA and LCA combined with testosterone have the potential to serve as biomarkers for the pathogenesis of PCOS.
Unraveling the periprandial changes in brain serotonergic activity and its correlation with food intake-related neuropeptides in rainbow trout Oncorhynchus mykiss
Authors: Chivite Mauro , Ceinos Rosa M. , Cerdá-Reverter José M. , Soengas Jose L. , Aldegunde Manuel , López-Patiño Marcos A. , Míguez Jesús M.
Published: August 2023

This study explored changes in brain serotonin content and activity together with hypothalamic neuropeptide mRNA abundance around feeding time in rainbow trout, as well as the effect of one-day fasting. Groups of trout fed at two (ZT2) and six (ZT6) hours after lights on were sampled from 90 minutes before to 240 minutes after feeding, while additional groups of non-fed trout were also included in the study. Changes in brain amine and metabolite contents were measured in hindbrain, diencephalon and telencephalon, while in the diencephalon the mRNA abundance of tryptophan hydroxylase (tph1, tph2), serotonin receptors (5htr1a, 5htr1b and 5htr2c) and several neuropeptides (npy, agrp1, cartpt, pomca1, crfb) involved in the control of food intake were also assessed. The results showed changes in the hypothalamic neuropeptides that were consistent with the expected role for each in the regulation of food intake in rainbow trout. Serotonergic activity increased rapidly at the time of food intake in the diencephalon and hindbrain and remained high for much of the postprandial period. This increase in serotonin abundance was concomitant with elevated levels of pomca1 mRNA in the diencephalon, suggesting that serotonin might act on brain neuropeptides to promote a satiety profile. Furthermore, serotonin synthesis and neuronal activity appear to increase already before the time of feeding, suggesting additional functions for this amine before and during food intake. Exploration of serotonin receptors in the diencephalon revealed only small changes for gene expression of 5htr1b and 5htr2c receptors during the postprandial phase. Therefore, the results suggest that serotonin may play a relevant role in the regulation of feeding behavior in rainbow trout during periprandial time, but a better understanding of its interaction with brain centers involved in receiving and processing food-related signals is still needed.
Nematicidal effect of Beauveria species and the mycotoxin beauvericin against pinewood nematode Bursaphelenchus xylophilus
Authors: Sánchez-Gómez Tamara , Harte Steven J. , Zamora Paula , Bareyre Matéo , Díez Julio Javier , Herrero Baudilio , Niño-Sánchez Jonathan , Martín-García Jorge
Published: August 2023

Introduction and main objective

Bursaphelenchus xylophilus, commonly known as pine wood nematode (PWN), is considered one of the greatest threats to European and Asian pines. Regarding its management, most efforts have been directed toward control measures for the major vector (Monochamus spp.) and screening for genetic resistance in its hosts. However, an integrated pest management strategy which also implements pinewood nematode control is currently lacking. The aim of this study was to evaluate the nematicidal effect of two Beauveria species, a genus well known for its entomopathogenic activity.

Summary methodology

For this purpose, in vitro antagonism tests of fungi (Beauveria bassiana and B. pseudobassiana) and the mycotoxin beauvericin (C45H57N3O9) on B. xylophilus populations were conducted. Finally, the production of beauvericin in B. bassiana and B. pseudobassiana strains was quantified by high-performance liquid chromatography - mass spectrometry (HPLC-MS).

Results and discussion

Both the B. bassiana and B. pseudobassiana fungal species and the mycotoxin beauvericin showed a clear nematicidal effect on B. xylophilus populations, substantially reducing their survival rate and even attaining 100% mortality in one case. HPLC-MS analysis confirmed and quantified the production of beauvericin by B. bassiana and demonstrated for the first-time beauvericin production in B. pseudobassiana.

Final conclusion

These findings highlight the potential of Beauveria species and the mycotoxin beauvericin to be implemented in an integrated pest management strategy to control both nematode and vector.
A multi-threaded approach to genotype pattern mining for detecting digenic disease genes
Authors: Zhang Qingrun , Bhatia Muskan , Park Taesung , Ott Jurg
Published: August 2023

To locate disease-causing DNA variants on the human gene map, the customary approach has been to carry out a genome-wide association study for one variant after another by testing for genotype frequency differences between individuals affected and unaffected with disease. So-called digenic traits are due to the combined effects of two variants, often on different chromosomes, while individual variants may have little or no effect on disease. Machine learning approaches have been developed to find variant pairs underlying digenic traits. However, many of these methods have large memory requirements so that only small datasets can be analyzed. The increasing availability of desktop computers with large numbers of processors and suitable programming to distribute the workload evenly over all processors in a machine make a new and relatively straightforward approach possible, that is, to evaluate all existing variant and genotype pairs for disease association. We present a prototype of such a method with two components, Vpairs and Gpairs, and demonstrate its advantages over existing implementations of such well-known algorithms as Apriori and FP-growth. We apply these methods to published case-control datasets on age-related macular degeneration and Parkinson disease and construct an ROC curve for a large set of genotype patterns.
Insights on the performance of sintered silt ash-blended cement: experiments and thermodynamic simulation
Authors: Li Zhipeng , Liu Zhengxiang , Zhang Hongguang , Luo Qiling , Long Wu-Jian
Published: August 2023

The dredged silt exposed to the air is highly expected to be used to replace cement in large quantities due to its richness in aluminosilicates, which is in line with the dual-carbon background. Herein, a systematic exploration on the basic properties and hydration products of sintered silt ash-blended (SSA-blended) cement is conducted, using experiments and thermodynamic simulations. The results show that in the process of adding SSA to 50%, the compressive property and chloride ion permeation resistance are enhanced first and then weakened, whereas the flexural property is weakened gradually. Among them, with the addition of 10% SSA, the compressive strength can reach 65.2 MPa and the electric flux is 37.3% lower than that of Portland cement (PC), and the chloride ion permeability is rated as the lowest. As SSA increases, the FTIR spectra show that the reduction rates of bound water, ettringite and Ca(OH)2 are accelerated gradually; SEM observation shows that the cement-based gelling skeleton has undergone a transition from dense to loose; the thermodynamic simulation results show that C-S-H increases first and then decreases, implying that SSA has a certain pozzolanic activity. In addition, the evolution laws of other hydration products are highly consistent with the analysis results of FTIR and SEM.
Development and validation of a nomogram for predicting persistent inflammation, immunosuppression, and catabolism syndrome in trauma patients
Authors: Xu Ligang , Kang Zhaofeng , Wang Dongfang , Liu Yukun , Wang Chuntao , Li Zhanfei , Bai Xiangjun , Wang Yuchang
Published: August 2023

Background

Persistent Inflammation, Immunosuppression, and Catabolism Syndrome (PIICS) is a significant contributor to adverse long-term outcomes in severe trauma patients.

Objective

The objective of this study was to establish and validate a PIICS predictive model in severe trauma patients, providing a practical tool for early clinical prediction.

Patients and methods

Adult severe trauma patients with an Injury Severity Score (ISS) of ≥16, admitted between October 2020 and December 2022, were randomly divided into a training set and a validation set in a 7:3 ratio. Patients were classified into PIICS and non-PIICS groups based on diagnostic criteria. LASSO regression was used to select appropriate variables for constructing the prognostic model. A logistic regression model was developed and presented in the form of a nomogram. The performance of the model was evaluated using calibration and ROC curves.

Results

A total of 215 patients were included, consisting of 155 males (72.1%) and 60 females (27.9%), with a median age of 51 years (range: 38–59). NRS2002, ISS, APACHE II, and SOFA scores were selected using LASSO regression to construct the prognostic model. The AUC of the ROC analysis for the predictive model in the validation set was 0.84 (95% CI 0.72–0.95). The Hosmer-Lemeshow test in the validation set yielded a χ2 value of 14.74, with a value of p of 0.098.

Conclusion

An accurate and easily implementable PIICS risk prediction model was established. It can enhance risk stratification during hospitalization for severe trauma patients, providing a novel approach for prognostic prediction.
Characterization of a novel galectin in Sarcoptes scabiei and its role in regulating macrophage functions
Authors: He Ran , Zhang Qian , Xu Luyang , Guo Maochuan , Gu Xiaobin , Xie Yue , Xu Jing , Shen Zhaoli
Published: August 2023

Sarcoptes scabiei (S. scabiei) endangers human and other mammalian health. There has been limited research into S. scabiei pathogenic mechanisms and the immunological interaction between S. scabiei and hosts. Galectins have critical roles in biological processes such as cell adhesion, signal transduction, and immune response mediation. Galectins of S. scabiei (SsGalectins) were cloned, expressed, and identified, and their transcriptional levels in S. scabiei were measured at various developmental stages. Fluorescent tissue localization was performed on SsGalectins of S. scabiei and scabies skin. A mouse AD model was constructed to evaluate the effect of rSsGalectins on skin pathogenic changes. Quantitative polymerase chain reaction and enzyme-linked immunoassay were used to identify macrophage polarization-related components and investigate the immunoregulatory effect of rSsGalectins on mouse macrophages. The results demonstrated that the S. scabiei infection causes macrophage infiltration in the scabies skin. The rSsGalectins displayed strong reactogenicity, and distinct genes of the SsGalectins were differently expressed in different developmental stages of S. scabiei. Fluorescence tissue localization revealed that the SsGalectins were mainly in the mouthparts, intestines, and body surface. Additionally, S. scabiei could secrete SsGalectins into the infected skin, proving that SsGalectins were excretion and secretion proteins of S. scabiei. In the mouse atopic dermatitis model, cutaneous macrophage infiltration and inflammation increase after rSsGalectins injection. Simultaneously, when rSsGalectins acted on bone marrow-derived macrophages, M1 macrophage-related polarization factors IL-1β, IL-6, and inducible nitric oxide synthase all increased, demonstrating that rSsGalectins can induce M1 polarization and produce pro-inflammatory cytokines. In conclusion, the SsGalectins are involved in the pathogenic process of S. scabiei by regulating the polarization of host macrophages to the M1 type when S. scabiei invade the host and promoting the incidence and development of the host's inflammatory response. This study offers fresh light on the pathogenic process of scabies mites, investigates the immunological interaction mechanism between S. scabiei and the host, and offers new insights into S. scabiei prevention and therapy.
The role of Bacillus Calmette-Guérin administration on the risk of dementia in bladder cancer patients: a systematic review and meta-analysis
Authors: Han Chao , Wang Juan , Chen Ya-Li , Guan Cui-Ping , Zhang Yan-An , Wang Mao-Shui
Published: August 2023

Background

Previous cohort studies have found an association between Bacillus Calmette–Guérin (BCG) administration and incident dementia. In the systematic review and meta-analysis, we aimed to summarize the current evidence of the effect of BCG use on the risk of developing dementia.

Methods

We searched six databases until 20 May 2023 for studies investigating the risk of dementia and BCG administration. Hazard ratios (HRs) and 95% confidence intervals (95% CIs) were pooled in the meta-analysis. Meta-regression, subgroup, and sensitivity analysis were conducted as well.

Results

Of the 4,043 records initially evaluated, five articles were included for final analysis, with a total of 45,407 bladder cancer (BC) patients. All five studies were evaluated and rated as with high quality, and a low possibility of publication bias was indicated. A significant association between BCG and the incidence of dementia in BC patients was found in all five studies. Although a high heterogeneity (I2 = 84.5%, p < 0.001) was observed, the pooled HR was 0.55 (0.42–0.73), indicating that BCG exposure or treatment reduced the risk of incident dementia by 45%. Moreover, the sensitivity analysis showed good robustness of the overall effect with no serious publication bias.

Conclusion

BCG administration is associated with a significantly lower risk of developing dementia. However, an epidemiological cohort is needed to establish a relationship between BCG use and incident dementia in the normal population. Once the relationship is confirmed, more people may benefit from the association.

Systematic review registration

identifier: CRD42023428317.
The Shank3-InsG3680(+/+) mouse model of autism spectrum disorder displays auditory avoidance in a novel behavioral test
Authors: Gonçalves Ana Margarida , Sousa Nuno , Jacinto Luis , Monteiro Patricia
Published: August 2023

Introduction

Autism spectrum disorder (ASD) is characterized by deficits in communication and social interaction, restricted interests, repetitive behaviors, and sensory alterations, with auditory hypersensitivity being one of the most commonly reported sensory–perceptual abnormalities. Several candidate genes for involvement in this disorder have emerged from patient studies, including SHANK3, a gene that encodes a protein (SHANK3) in the postsynaptic density of excitatory synapses. Previous work has shown that mutant mice carrying a human ASD mutation in the Shank3 gene (InsG3680) exhibit repetitive behaviors and social interaction deficits, indicating important construct and face validity for this genotype as an animal model of ASD.

Methods

To further address whether these mice also present auditory sensory–perceptual alterations, we developed a novel behavioral test in which mice can choose between different soundscapes.

Results

Our results reveal that, in comparison to wild-type mice, Shank3 mutants display a strong behavioral preference toward silent regions of the arena.

Discussion

These data suggest that Shank3- mutant mice might express an auditory hypersensitivity phenotype, further adding to the face validity of this genotype as an animal model of ASD.
Quantifying conditioned place preference: a review of current analyses and a proposal for a novel approach
Authors: Yates Justin R.
Published: August 2023

Conditioned place preference (CPP) is used to measure the conditioned rewarding effects of a stimulus, including food, drugs, and social interaction. Because various analytic approaches can be used to quantify CPP, this can make direct comparisons across studies difficult. Common methods for analyzing CPP involve comparing the time spent in the CS+ compartment (e.g., compartment paired with drug) at posttest to the time spent in the CS+ compartment at pretest or to the CS– compartment (e.g., compartment paired with saline) at posttest. Researchers can analyze the time spent in the compartment(s), or they can calculate a difference score [(CS+post – CS+pre) or (CS+post – CS–post)] or a preference ratio (e.g., CS+post/(CS+post + CS–post)). While each analysis yields results that are, overall, highly correlated, there are situations in which different analyses can lead to discrepant interpretations. The current paper discusses some of the limitations associated with current analytic approaches and proposes a novel method for quantifying CPP, the adjusted CPP score, which can help resolve the limitations associated with current approaches. The adjusted CPP score is applied to both hypothetical and previously published data. Another major topic covered in this paper is methodologies for determining if individual subjects have met criteria for CPP. The paper concludes by highlighting ways in which researchers can increase transparency and replicability in CPP studies.
Uncertainty-aware automated assessment of the arm impedance with upper-limb exoskeletons
Authors: Tesfazgi Samuel , Sangouard Ronan , Endo Satoshi , Hirche Sandra
Published: August 2023

Providing high degree of personalization to a specific need of each patient is invaluable to improve the utility of robot-driven neurorehabilitation. For the desired customization of treatment strategies, precise and reliable estimation of the patient's state becomes important, as it can be used to continuously monitor the patient during training and to document the rehabilitation progress. Wearable robotics have emerged as a valuable tool for this quantitative assessment as the actuation and sensing are performed on the joint level. However, upper-limb exoskeletons introduce various sources of uncertainty, which primarily result from the complex interaction dynamics at the physical interface between the patient and the robotic device. These sources of uncertainty must be considered to ensure the correctness of estimation results when performing the clinical assessment of the patient state. In this work, we analyze these sources of uncertainty and quantify their influence on the estimation of the human arm impedance. We argue that this mitigates the risk of relying on overconfident estimates and promotes more precise computational approaches in robot-based neurorehabilitation.
A patient with MELAS syndrome combined with autoimmune abnormalities: a case report
Authors: Zhao Mingmin , Zuo Chun , Hao Hongyu , Xing Xing , Zhao Lei , Li Na
Published: August 2023

Background

Mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS) is a group of maternally inherited disorders caused by mutations or deletions in mitochondrial genes with mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes as the main clinical manifestations.

Case presentation

We reported a 20-year-old female patient with MELAS syndrome combined with autoimmune abnormalities. She suffered from an intermittent headache in the right temporal region with no obvious cause, and then, after strenuous exercise in dance class, the headache became aggravated, accompanied by unresponsiveness, blurred vision, and diplopia. Her blood lactate levels were elevated, her antinuclear antibodies were positive, and the antimetabolic glutamate receptors 5 in her serum were positive. Brain DWI showed a hypertensive signal in the right temporo-parietal-occipital cortex and subcortical area. Brain MRS showed decreased NAA peak and increased Lac peak. Muscle biopsy showed myogenic damage, and the modified Gomori trichrome (MGT) staining showed ragged red fibers (RRF). A genetic study revealed a mitochondrial DNA A3243G mutation.

Conclusion

Mitochondrial encephalomyopathy is a rare clinical condition; however, the association with autoimmune diseases is not yet clear and still needs further research and analysis.
SHH medulloblastoma and very early onset of bowel polyps in a child with PTEN hamartoma tumor syndrome
Authors: Caroleo Anna Maria , Rotulo Silvia , Agolini Emanuele , Macchiaiolo Marina , Boccuto Luigi , Antonelli Manila , Colafati Giovanna Stefania , Cacchione Antonella , Megaro Giacomina , Carai Andrea , De Ioris Maria Antonietta , Lodi Mariachiara , Tornesello Assunta , Simone Valeria , Torroni Filippo , Cinalli Giuseppe , Mastronuzzi Angela
Published: August 2023

Phosphatase and tensin homolog (PTEN) hamartoma tumor syndrome (PHTS) is a cancer predisposition syndrome characterized by an increased risk of developing benign and malignant tumors, caused by germline pathogenic variants of the PTEN tumour suppressor gene. PTEN gene variants often present in childhood with macrocephaly, developmental delay, and/or autism spectrum disorder while tumors and intestinal polyps are commonly detected in adults. PHTS is rarely associated with childhood brain tumors with only two reported cases of medulloblastoma (MB). We report the exceptional case of an infant carrying a germline and somatic pathogenic variant of PTEN and a germline and somatic pathogenic variant of CHEK2 who developed a MB SHH in addition to intestinal polyposis.
Challenges and opportunities for research clinicians interested in pain: results of a survey
Authors: Siddons Andrew , Wandner Laura Dover , Porter Linda L.
Published: August 2023

The National Institutes of Health and its independent advisors recognize the need to develop a strong pain research workforce and provide opportunities, particularly for clinicians, to pursue research careers. A survey was conducted to better understand the challenges facing the clinical pain research community. Respondents reported that time and funding to pursue research were the most critical factors either enabling or holding them back from a research career. Respondents who received some kind of formal research training or mentorship were more likely than those who did not to have federal research funding and to be at more advanced stages of their careers. The findings point to a need for all stakeholders in the pain research community to help formalize research training and provide funding or protected time to support the ambitions of aspiring researchers.
Vessels characteristics in familial exudative vitreoretinopathy and retinopathy of prematurity based on deep convolutional neural networks
Authors: Deng Xinyi , Chen Kun , Chen Yijing , Xiang Ziyi , Zhang Shian , Shen Lijun , Sun Mingzhai , Cai Lingzhi
Published: August 2023

Purpose

The purpose of this study was to investigate the quantitative retinal vascular morphological characteristics of Retinopathy of Prematurity (ROP) and Familial Exudative Vitreoretinopathy (FEVR) in the newborn by the application of a deep learning network with artificial intelligence.

Methods

Standard 130-degree fundus photographs centered on the optic disc were taken in the newborns. The deep learning network provided segmentation of the retinal vessels and the optic disc (OD). Based on the vessel segmentation, the vascular morphological characteristics, including avascular area, vessel angle, vessel density, fractal dimension (FD), and tortuosity, were automatically evaluated.

Results

201 eyes of FEVR, 289 eyes of ROP, and 195 eyes of healthy individuals were included in this study. The deep learning system of blood vessel segmentation had a sensitivity of 72% and a specificity of 99%. The vessel angle in the FEVR group was significantly smaller than that in the normal group and ROP group (37.43 ± 5.43 vs. 39.40 ± 5.61, 39.50 ± 5.58, P = 0.001, < 0.001 respectively). The normal group had the lowest vessel density, the ROP group was in between, and the FEVR group had the highest (2.64 ± 0.85, 2.97 ± 0.92, 3.37 ± 0.88 respectively). The FD was smaller in controls than in the FEVR and ROP groups (0.984 ± 0.039, 1.018 ± 0.039 and 1.016 ± 0.044 respectively, P < 0.001). The ROP group had the most tortuous vessels, while the FEVR group had the stiffest vessels, the controls were in the middle (11.61 ± 3.17, 8.37 ± 2.33 and 7.72 ± 1.57 respectively, P < 0.001).

Conclusions

The deep learning technology used in this study has good performance in the quantitative analysis of vascular morphological characteristics in fundus photography. Vascular morphology was different in the newborns of FEVR and ROP compared to healthy individuals, which showed great clinical value for the differential diagnosis of ROP and FEVR.
Effect of anti-obesity agent HSG4112 on overweight and obese patients following 12 weeks of oral treatment: a study protocol for a randomised, double-blind, placebo-controlled, parallel-group, phase 2a clinical trial
Authors: Min Kyungha , Oh Bumjo , Koo Hye Yeon , Kim Yang-Hyun , Lee Ji-Won , Lee Sangsub , Kim Youngah , Kwon Hyuktae
Published: August 2023

Background: Glaceum Inc. has proposed HSG4112, a structural analogue of glabridin, as a novel anti-obesity compound. Animal studies and phase I human trials have shown that HSG4112 improves energy consumption, normalises weight, and is safe and drug-resistant. Based on these results, the company plans to conduct a phase 2a clinical trial to determine the safety and efficacy of HSG4112 in overweight and obese patients. Methods: A 16-week randomised, double-blind, placebo-controlled, parallel-group trial will be conducted at five large hospitals in South Korea to assess the safety and efficacy of HSG4112 in overweight and obese patients. Participants who meet the inclusion/exclusion criteria will be assigned a subject number and randomly assigned to one of the four treatment groups (one group receiving a placebo) in a 1:1:1:1 ratio. The study’s primary outcome will be to monitor the change in body weight (kg) from baseline to the end of treatment while monitoring safety and tolerability. Discussion: This trial will evaluate the efficacy and safety of HSG4112 in overweight and obese adults. Upon proving the safety and effectiveness of the newly developed mechanism, it might significantly improve the perception of the product among medical personnel and obese patients. Furthermore, it may aid in managing chronic conditions that require long-term treatment.

Trial registration:ClinicalTrials.gov, identifier [NCT05197556].
An effective prognostic model in colon adenocarcinoma composed of cuproptosis-related epigenetic regulators
Authors: Liu Yang , Wang Yizhao , Li Chang , Feng Huijin , Liu Yanqing , Ma Lianjun
Published: August 2023

Background: Colorectal adenocarcinoma (COAD) is a common malignant tumor with little effective prognostic markers. Cuproptosis is a newly discovered mode of cell death that may be related to epigenetic regulators. This study aimed to explore the association between epigenetic regulators and cuproptosis, and to establish a prognostic prediction model for COAD based on epigenetic regulators associated with cuproptosis (EACs).

Methods: RNA sequencing data and clinical data of 524 COAD patients were obtained from the TCGA-COAD database, cuproptosis-related genes were from the FerrDb database, and epigenetic-related genes were from databases such as GO and EpiFactors. LASSO regression analysis and other methods were used to screen out epigenetic regulators associated with cuproptosis and prognosis. The risk score of each patient was calculated and the patients were divided into high-risk group and low-risk group. Next, the survival difference, functional enrichment analyses, tumor mutation burden, chemotherapy drug sensitivity and other indicators between the two groups were compared and analyzed.

Results: We found 716 epigenetic regulators closely related to cuproptosis, among which 35 genes were related to prognosis of COAD. We further screened out 7 EACs from the 35 EACs to construct a prognostic prediction model. We calculated the risk score of each patient based on these 7 genes, and divided the patients into high-risk group and low-risk group. We found that the overall survival rate and progression-free survival rate of the high-risk group were significantly lower than those of the low-risk group. This model showed good predictive ability in the training set, test set and overall data set. We also constructed a prognostic prediction model based on risk score and other clinical features, and drew the corresponding Nomogram. In addition, we found significant differences between the high-risk group and the low-risk group in tumor mutation burden, chemotherapy drug sensitivity and other clinical aspects.

Conclusion: We established an effective predictive prediction model for COAD based on EACs, revealing the association between epigenetic regulators and cuproptosis in COAD. We hope that this model can not only facilitate the treatment decision of COAD patients, but also promote the research progress in the field of cuproptosis.
Construction and application of prone position ventilation management scheme for severe COVID-19 patients
Authors: Chen Xiuwen , Peng Cao , Xiao Yao , Liu Shiqing
Published: August 2023

Background: Prone position ventilation (PPV) can significantly improve oxygenation index and blood oxygen saturation in most (70%–80%) patients with acute respiratory distress syndrome. However, although PPV is not an invasive procedure, there are many potential PPV-related complications, such as nerve compression, crush injury, venous stasis (e.g., facial oedema), pressure sores, retinal damage, vomiting, and arrhythmia, with an incidence of up to 56.9%. Nursing managers have focused on reducing the occurrence of PPV-related complications and improving safety.

Objective: To construct a prone ventilation management scheme for patients with severe coronavirus disease 2019 (COVID–19) and analyse its application effect.

Methods: Based on a previous evidence-based study combined with the COVID-19 Diagnosis and Treatment Protocol (Trial Edition 9), a prone ventilation management protocol for severe COVID-19 was formulated and applied to COVID-19 patients in the intensive care unit of a designated hospital. A prospective self-control study was used to compare changes in the oxygenation index and other outcome indicators before and after the intervention.

Results: The oxygenation index of patients after intervention (321.22 ± 19.77 mmHg) was significantly higher (p < 0.05) than before intervention (151.59 ± 35.49 mmHg). The difference in oxygenation index in different prone position ventilation durations was statistically significant (p < 0.05). Nursing quality evaluation indicators showed that the implementation rate of gastric residual volume assessment was 100% and the incidence of occupational exposure and cross-infection was 0%; the incidences of pressure ulcers, drug extravasation, and facial oedema were 13.64% (3/22), 4.54% (1/22), and 4.54% (1/22), respectively. The incidence of unplanned extubation, aspiration, and falls/falls was 0%.
Important but challenging: Chinese in-service EFL teachers’ research attitudes in a graduate program
Authors: Chen Lei , Liu Quanguo
Published: August 2023

In recent decades, the practice of involving teachers in research in degree programs is becoming popular. Yet, little is known about the impact of research experiences on teachers’ behavior: whether research experiences change their teaching practices and lead to further research efforts in future careers, especially in the unique social, cultural, and educational culture of China. Thus, this study examines Chinese IETs’ (in-service EFL teachers’) research attitudes in a graduate program with a reasoned action approach. We used an embedded mixed methods research design to investigate a cohort of 197 IETs who completed the Research Attitudes in Vocational Education Questionnaire (RAVE-Q). The quantitative data validates the survey and shows that, in general, the IETs hold positive attitudes toward research. Next, semi-structured interviews investigating IETs and their advisors’ perceptions of teacher research were conducted. The qualitative data shows a variety of research experiences among the IETs. Specifically, this study highlighted some interviewed IETs who had the desire to be reflective about their teaching and to apply research in their practices, nevertheless, the educational contexts made such efforts impossible. Thus, this study questioned the previous assumptions that positive research attitudes lead to corresponding research behaviors. This study offers implications for EFL graduate programs seeking to improve IETs’ research attitudes both within and outside China.
Combining differentiating and integrating over time to sustain multiple institutional logics: a case study of a higher education institution
Authors: Jiang Ye , Zhang Xiaojun
Published: August 2023

To understand how organizations adopt varying configurations of differentiating (i.e., compartmentalizing logics into different subunits or roles) and integrating (i.e., combining logics to highlight synergies) over time to navigate logic contestations and extract logic complementarities for organizational innovation, we conduct a 15-year in-depth longitudinal case study of a higher education institution to examine how it devised innovative hybrid practices to manage and support college student development. By employing the grounded theory method, we develop a four-stage process model of the varying configurations of differentiating and integrating that expounds on how two contested logics are sustained and exploited over time. We assert that selective bridging—the instrumental use of one logic enables some practices of one logic to be selectively integrated with the other, while irreconcilable practices remain differentiated to play their respective roles, is vital in allowing organizations to leverage respective advantages in combining differentiating and integrating over time. Further, we show that combining integrating and differentiating features simultaneously transforms contested logics into compatible and complementary, offering a novel way for logic hybridization. These findings advance the understanding of how organizations can differentiate and integrate contested logics over time through a dynamic and paradoxical view, thus being manageable and manipulated for organizational innovation.
Child self-care autonomy in health (scale for parents): development, internal structure, and sex/age correlates
Authors: Mikhaylova Oxana , Bochkor Anastasia , Osipova Polina , Popov Denis , Chepeleva Maria , Rybakova Evgenia
Published: August 2023

Autonomy in self-care practices in the health sphere is a critical characteristic for the survival of humans throughout the life span. Notably, however, the current literature lacks psychometrically sound instruments that measure this phenomenon among children without diagnosed chronic health conditions. The purpose of the present exploratory study was to develop, test, and provide data regarding the reliability and validity of the Child Self-Care Autonomy in Health (CSAH) scale. The piloted version of the CSAH yielded an 11-item instrument designed to reflect the parent’s perspective in measuring the extent of autonomy in self-care actions related to health for a child, whether diagnosed with a chronic illness or not. Data were collected through an online survey of a non-random sample of Russian-speaking parents currently residing in Russia (N = 349). The analysis focused on scale structure via principal component analysis and age/sex associations. The proposed CSAH may be of interest to social workers, health professionals, and parents seeking to ascertain schoolers’ autonomy in self-care practices in the health sphere and support building a stronger self-care mindset.
Elementary school staff perspectives on the implementation of physical activity approaches in practice: an exploratory sequential mixed methods study
Authors: Walker Timothy J. , Pfledderer Christopher D. , Craig Derek W. , Robertson Michael C. , Heredia Natalia I. , Bartholomew John B.
Published: August 2023

Introduction

A whole-of-school approach is best to promote physical activity before, during, and after school. However, multicomponent programming is often complex and difficult to deliver in school settings. There is a need to better understand how components of a whole-of-school approach are implemented in practice. The objectives of this mixed methods study were to: (1) qualitatively explore physical activity approaches and their implementation in elementary schools, (2) quantitatively assess implementation levels, and (3) examine associations between school-level physical activity promotion and academic ratings.

Methods

We used an exploratory sequential mixed methods design. We conducted semi-structured qualitative interviews with elementary school staff from a Texas school district and used a directed content analysis to explore physical activity approaches and their implementation. Using qualitative findings, we designed a survey to quantitatively examine the implementation of physical activity approaches, which we distributed to elementary staff district wide. We used Pearson correlation coefficients to examine the association between the amount of physical activity opportunities present in individual schools and school-level academic ratings.

Results

We completed 15 interviews (7 principals/assistant principals, 4 physical educators, and 4 classroom teachers). Elementary school teachers and staff indicated PE and recess implementation was driven from the top-down by state and district policies, while implementation of classroom-based approaches, before and after school programming, and active transport were largely driven from the bottom-up by teachers and school leaders. Teachers and staff also discussed implementation challenges across approaches. Survey respondents (n = 247 from 22 schools) indicated 54.6% of schools were implementing ≥135 min/week of physical education and 72.7% were implementing 30 min/day of recess. Classroom-based approaches were less common. Twenty-four percent of schools reported accessible before school programs, 72.7% reported accessible after school programs, and 27% promoted active transport. There was a direct association between the number of physical activity opportunities provided and school-level academic ratings r(22) = 0.53, p = 0.01.

Conclusion

Schools provided physical activity opportunities consistent with a whole-of-school approach, although there was variability between schools and implementation challenges were present. Leveraging existing school assets while providing school-specific implementation strategies may be most beneficial for supporting successful physical activity promotion in elementary schools.
Using mobile health to encourage physical activity in individuals with intellectual disability: a pilot mixed methods feasibility study
Authors: Michalsen Henriette , Henriksen André , Pettersen Gunn , Hartvigsen Gunnar , Wangberg Silje , Thrane Gyrd , Jahnsen Reidun , Anke Audny
Published: August 2023

Background

Many individuals with intellectual disability (ID) have a sedentary lifestyle. Few interventions aimed at increasing their level of physical activity (PA) have shown lasting effects.

Aim

To assess the feasibility and acceptability of a pilot intervention study using innovative mobile health (mHealth) support systems to encourage PA in individuals with ID.

Methods

Nine individuals with ID and a low level of PA, aged 16–36 years, were included in the present convergent triangulation mixed method design. Two mHealth support systems (apps) were developed and tested. PA was measured with a Fitbit smartwatch, accelerometer, the International Physical Activity Questionnaire—Short Form (IPAQ-S), and Goal attainment scaling. Data were collected through online pre-, mid- (4 weeks), and post-intervention (12 weeks) questionnaires and activity trackers. Semi-structured qualitative interviews with participants and/or a family or staff member were held after the 12-week follow-up. Data were analyzed using conventional nonparametric statistics and thematic analyses.

Results

The response rate and retention to the trial were 16% and 100%, respectively. Data quality was high, except for missing data from Fitbit activity trackers of approximately 30% from the 4- and 12-week follow-up stages. The feasibility challenges with activity trackers include rashes, size, non-acceptance, and loss of motivation. Participants and family members/staff reported interest in the study theme and were pleased with the data collection method. All but one participant achieved their PA goals. Most participants reported being satisfied with the apps as they were enjoyable or provided a reminder for performing physical and other activities. Social support for PA among family members also increased. However, app support from staff and family members was needed, and apps were not used regularly. Two of nine participants (22%) had increased their PA measured as steps per day with Fitbit at the 12-week follow-up.

Conclusions

The acceptability and feasibility of using tailored mobile applications in natural settings to increase PA among adults with ID are promising. This study aligns with previous studies in showing the challenges to increasing PA, which requires the inclusion of family members, staff, and stakeholders. The intervention requires modifications before a randomized controlled trial can be conducted.