bluuebunny/crossref_metadata_2025 huggingface dataset:
Chromosomal mechanisms in adapting Allium hymenorhizum Ledeb. under introduction conditions
Authors: Kalashnik N A , Kutueva A G , Maslova N V , Elizaryeva O A , Muldashev A A
Published: January 2020

The paper presents a karyotypicel description of the rare relict species Allium hymenorhizum Ledeb. illustrated by three samples introduced to the South Ural Botanical Garden-Institute from the Bashkir Cis-Ural and South Ural regions. It was found out that the chromosome number in somatic tissue of this species is 2n=16; chromosomes are of medium size (6.05±0.15 to 9.35±0.22 μm) and belong to the metacentric and submetacentric types. The variability of chromosomal morphometric parameters is characterized by the extremely low, low and average values of the coefficients of variation. The samples introduced from different climatic conditions vary in the number and frequency of secondary constrictions in the chromosomal complements, this evidently being associated with the processes of their adaptation to new growing conditions.
A new look at the etiological structure of pig streptococcosis
Authors: Terekhov P Yu , Matyash E A , Yakimova E A , Kapustin A V , Belyaeva A S , Laishevtsev A I
Published: January 2020

Streptococcosis of pigs is an urgent problem for the pig industry in Russia, manifested in various forms of pathology, causing significant economic damage to enterprises and to the country as a whole. Due to the high tropism of bacteria of the genus Streptococcus, infection can occur in various clinical forms, which is also caused by the initial localization of pathogens such as in the tonsils and nasal cavity of animals, including normal microflora, in the genitourinary system and in the digestive tract. Along with epizootic significance, pathogenic streptococci have epidemiological significance, since pathogenicity extends not only to animals, but also to humans. Within the framework of state epizootic monitoring in Russia the Streptococcus infection is not considered as controlled one, which is why it is difficult to analyze the current prevalence of streptococci, the modern etiological structure of the causative agents of the disease and the features of its epizootic process. So, for a long time, it was believed that pig streptococcosis is caused solely by the species Streptococcus suis, but this conclusion is outdated in reality and needs to be reviewed. In particular, as a result of the work carried out, at least 22 species of bacteria of the genus Streptococcus circulate in pig breeding complexes in Russia, of which Streptococcus dysgalactiae (two subspecies), Streptococcus porcinus, Streptococcus suis, Streptococcus pyogenes, Streptococcus are most often identified alactolyticus, Streptococcus entericus, etc. These isolates were isolated from animals exhibiting any clinical manifestations or pathological changes (posthumous) characteristic of streptococcal infections. When determining the pathogenic properties of the isolated streptococcal isolates, it was determined that most cultures of Streptococcus dysgalactiae, Streptococcus porcinus, Streptococcus suis, Streptococcus pyogenes cause the death of laboratory animals (white mice with the intraperitoneal method of infection), which indicates the ability of these species to provoke pathogen diseases. Thus, the results suggest that the species Streptococcus suis should not be considered the only etiologically significant causative agent of streptococcosis in pigs, and the system of treatment and preventive measures at disease-favorable enterprises should be adjusted, taking into account the actualized this work information.
Legal and organizational basis of aviary keeping of animals
Authors: Moiseeva T A
Published: January 2020

Aviary keeping of wild animals is now becoming more widespread and is used for a variety of purposes: environmental, hunting, agricultural, aesthetic, educational. The article deals with the main Russian regulatory laws, regulations, orders regulating the enclosure of animals, gives a brief analysis and evaluation. The procedure for issuing permits for the maintenance and breeding of hunting resources in semi-wild conditions and artificially created habitat is considered in stages. The scheme of state and public control over the maintenance of animals is considered separately. It is concluded that the organization and technology of work on the preparation and maintenance of the aviary complex must comply with the existing civil legislation, but in the Russian legislation, the aviary kepping today does not have a clear definition and regulation.
Efficiency of crowdfunding as institution of global market for venture capital
Authors: Rutskiy V N , Tsarev R Y , Titov I A
Published: January 2020

The paper discusses the effectiveness of crowdfunding institution. Crowdfunding is the result of evolutionary institutional changes in the global venture capital market. The crowdfunding institution has lower transaction costs and allocates risks more evenly in comparison with the institutions of business angel and venture fund. Regression models of the effectiveness of global fundraising campaigns with the help of three types of institutions were designed. Comparison of models makes it possible to conclude that crowdfunding as an effective mechanism for organizing venture transactions, which significantly expands the audience of investors and projects that attract investments in the early stages.
Agricultural products sales in the Arctic zone of the Krasnoyarsk Territory
Authors: Ozerova M G , Pyzhikova N I , Filimonova N G
Published: January 2020

In the article the problems of organization of traditional northern products sales are considered. An analysis of the activities of enterprises showed that most of them sell products within the region, and few go beyond it. Sale is limited to certain products limits, and many buy them from small businesses. In order to improve the sales efficiency and access to foreign markets, it is proposed to establish a wholesale distribution center that will, on the other hand, create jobs in the native-born population area.
Aspects of environmental safety improving of whole grain bakery products
Authors: Khmeleva E , Berezina N , Khmelev A , Kunitsyna T , Makarova N
Published: January 2020

Cereal grains, used as a whole for bread production, can be a source of potentially hazardous substances. The article presents averaged analytic data on the toxic elements content in 30 batches of wheat grown in the Orel region for 2015-2018. It is shown that in some cases grain contains lead, nickel and mercury in quantities exceeding permissible levels. The possibility of using enzyme preparations to reduce the pollution of grain by heavy metals in the technology of whole grain bread at the stage of grain steeping is investigated.
Creation of the automatic machine of the cell pathology recognizer
Authors: Kostarev S N , Sereda T G , Tatarnikova N A , Kochetova O V
Published: January 2020

The histology is carried out for studying of fabrics of different bodies and systems. The histologic research helps to define existence of pathological cells and new growths with high precision. Modern examinations of an organism are conducted in the different ways: surveys, analyses, ultrasonography, but not always these methods allow to make precisely the diagnosis or to find pathological processes at the cellular level. This method is often applied in various fields of medicine and veterinary science. We conducted pilot laboratory studies patholologically of the changed cells with use of a technique of the histologic analysis. Qualitative and quantitative characteristics pathologists of the changed cells, with identification of indicators are defined. The module of support of decision-making for the preliminary diagnosis of pathologies is developed. The table of the validity of communication of indicators and group of pathologies is developed. With use of the theory of finite-state machines minimization of disjunctive normal form is carried out. On the basis of the received logical equations ladder charts for programming of logical matrixes of the microcontroller of OMRON are constructed. Experiments on reliability of results of the automatic machine - the recognizer of pathology of cells, the shown satisfactory results are made.
Issues of compatibility of processor command architectures
Authors: Zmyzgova T R , Solovyev A V , Rabushko A G , Medvedev A A , Adamenko Yu V
Published: January 2020

Modern computers and computing devices are based on the principle of open architecture, according to which the computer consists of several sufficiently independent devices that perform a certain function. These devices must meet certain standards of interaction with each other. Existing standards relate to both the technical characteristics of the devices and the content of the signals exchanged between them. The article considers the issue of creating a universal architecture of processor commands. The brief analysis of the components of devices and interrelations between them (different hardware features of processors, architecture of memory models and registers of peripheral devices, mechanisms of operand processing, the number of registers and processed data types, interruptions, exceptions, etc.) is carried out. The problem of architecture standardization, which generalizes the capabilities of the most common architectures and is suitable for high-performance emulation on most computer architectures, is put.
Territorial differences and structural dynamics of the North Caucasus agriculture
Authors: Kushkhova B A , Bakkuev E S , Ivanova Z M , Tramova A M , Khochueva Z M
Published: January 2020

Agriculture of the North Caucasus is one of the most developed in Russia. At the same time, within this macro-region there are noticeable (and in some aspects strong) differentiations not only in terms of the so-called production specialization, volumes of production, export, import, but also in terms of development (productivity, productivity, profitability, etc.). The reason for the latter is a complex of climatic, spatial, organizational, technological, technical, institutional, logistics and so on. factors and conditions of agricultural organization. The latter affect both the general development path of North Caucasian agriculture and the local paths of individual sectors and types of agricultural production in the macroregion with corresponding consequences for regional economies. However, this so-called a priori understanding of territorial differences needs to be clarified. In particular, it is known that the same factors (especially climatic, weather, etc.) lead to different returns on the so-called factor productivity. In addition, in reality it is important not only to indicate the presence of a particular factor, but to quantify its influence in specific conditions. In this regard, it is necessary to analyze and obtain specific (quantitative) data on territorial differences, determine the factors and conditions that form these differences and form features of the structural dynamics of agriculture in the North Caucasus.
The effectiveness of the modes of the super-high frequency electromagnetic field on the viability and infection of the second generation barley seeds
Authors: Kozulina N S , Vasilenko A A , Shmeleva Zh N
Published: January 2020

This article presents the results of experimental studies, the assessment of barley seeds contamination obtained from effective variants of exposure by the electromagnetic field of super-high frequency, tested in laboratory and field conditions, pathogens genera Bipolaris, Fusarium and Alternaria. The positive effect of the super-high frequency electromagnetic field is manifested not only in barley seeds treated immediately before sowing, but also persists in the second generation, i.e. after harvesting. At the same time, there is a significant decrease in the infection rate of samples for both external and internal infection.
The sources of power on TEG basis for digital technologies of agriculture
Authors: Dolgikh P P , Ibrogimov R I
Published: January 2020

The paper presents scientific and technical solutions to improve the efficiency of decentralized power supply systems for consumers that implement digital technologies in agriculture. The author’s technology is described, which makes it possible to evaluate the effectiveness of TEG in an extended temperature range, as well as to study the modes of their operation with electric load. The results are given that determine the rational temperature range for hot junction 160 … 200 °C, for cold - 30 … 50 °C. The dependence of the output power on the load current, described by a polynomial of the second degree, is obtained. The regularities of the influence of the load on TEG efficiency are determined. Based on the obtained experimental data, a power source circuit based on a heating device with TEG was developed.
The use of mathematical modeling tools to predict the yield of genetically modified crops
Authors: Lyapunova I A , Fomenko N A
Published: January 2020

Despite the high-tech innovation level of cultivation and processing, damage by insects - pests, is still high. In this connection, a sense of urgency growing new genetically modified (GM) varieties agricultures containing the gene of the soil bacterium called transgenic or genetically modified. This article explains the importance of mathematical modelling for the cultivation of GM crops.
Crops reclamation management based on hybrid neuro-fuzzy systems
Authors: Tokarev K E , Orlova Yu A , Rogachev A F , Kuzmin V A , Tokareva Yu M
Published: January 2020

The article deals the technique of application of neuro-fuzzy systems in problems of management of melioration of agricultural crops is considered. In particular, an adaptive network based on the fuzzy inference system is developed, a method for predicting data flows based on the neuro-fuzzy network is proposed, which is implemented in the MATLAB ANFIS module.
Investigation of elastic properties of helical springs of the control unit of the gearbox 25.01-15-20 SB
Authors: Medvedev V I , Ivanshchikov Yu V , Novikov A M , Pushkarenko N N , Dobrokhotov Yu N , Galtseva O V
Published: January 2020

Paper discuss the elastic properties of helical springs of the control unit of the gearbox 25.01-15-20 SB. During the analysis of the results of the study it was determined that the reason for the failure of the springs was the insufficient difference between the maximum shearing stress and the shearing stress under the working deformation. It was also found that the endurance of the cylindrical springs depends not only on the magnitude of the maximum operating voltage, but also on the amplitude of the oscillations themselves.
Artificial intelligence in the tourism sphere
Authors: Kazak A N , Chetyrbok P V , Oleinikov N N
Published: January 2020

The times of travel agents selecting tourist routes in the office seem to be finally passing. The potential of artificial intelligence (AI) technologies in the tourism industry exceeds the capabilities of traditional search engines and real people. Some travel services have already begun to use elements of artificial intelligence, which help to analyze large volumes of data and learn from their own and other people’s experience of fulfilling customer orders. Currently, the main goal for travel brands is to “learn” using personalized customer experience. Personalized services that are most suitable for a particular client are a strong competitive advantage. It is AI that helps choose such services, since it allows processing a lot of data and creating a personalized product much faster than traditional search technologies
Industrial product platforms and blockchain in aquaculture
Authors: Altoukhov A V
Published: January 2020

Modern technologies and new management concepts - industrial and product platforms - allows create new products and services, changes one of the oldest occupations of mankind, almost biblical, - fishing and fish farming. Due to the rapid development of IT technologies and business in the last decade, the development of platforms has been observed - network business structures engaged in the collection and analysis of heterogeneous information for users. The most important platforms for aquaculture are industrial and product. The newest concept of innovative economy – blockchain, - must be also used in modern industrial aquaculture. We propose the product platform based on an industrial platform, - software and hardware complex for fishes and crustaceans farming using Computer Vision technologies and modern automation systems integrated into a network industrial complex. The environmental parameters for optimizing the growth rate of aquatic organisms or their taste are subsequently distributed (license/sales) among users of their product platform. Platform users could collect fishes and crustaceans into the trading pools and sell them to large retailers for special prices. Product platform helps preserve, repopulate endangered species of fish and crustaceans and also reduces ecological impact of fisheries and extensive aquaculture.
The state of agrolandscapes of the forest-steppe zone of the Krasnoyarsk Territory
Authors: Badmaeva S E , Badmaeva Yu V
Published: January 2020

The landscape concept should give a fundamental assessment of the intensification extent of agricultural prospects. A detailed agroecological examination of all components of the natural-territorial complex, the creation of simulation models of their dynamics and the adoption of optimal decisions based on the results of forecasts should be carried out. The purpose of landscape analysis of natural resources is a comprehensive assessment of climatic, soil, plant and animal factors, establishing the relationship of landscape components with farming systems. Any landscape in the process of its development is exposed to impacts, and its stability has its limits. The stability threshold, expressed through the preservation by the landscape of its parameters and properties, and the critical impact quantities are determined in each case. General criteria for the natural stability of geosystems are their high organization, intensive functioning and geosystems functions balance, including biological productivity and renewability of vegetation cover. These qualities are determined by the optimum ratio of heat and moisture and are expressed by the development of soil cover, and ultimately, soil fertility. Landscape-ecological analysis of the territory makes it possible to establish the composition of agricultural and non-agricultural land, taking into account the heterogeneity and stability of a particular landscape, the occurrence of geochemical and biological processes in it, as well as to link the system of agriculture, land reclamation and specialization of the agricultural organization. The main element of Novosyolovo district landscape is agricultural land; the lands of the forest fund are the second largest area of natural geosystems. The degree of change in agrolandscapes is estimated on the basis of the existing structural and functional organization of agricultural lands, their quality state. It was established that plowing is low and it does not exceed the allowable limit, the forest cover of the district is close to optimal.
The use of poly- and monoclonal antibodies to assess the state of the immune system
Authors: Ezdakova I Yu , Kapustina O V , Popova E V , Grigoriev A G , Kovaikina V M
Published: January 2020

The function of the immune system of animals is influenced by a fairly large number of external (environmental, anthropogenic, infectious) and internal (mutations, impaired protein synthesis) factors. The result of their exposure is either the activation of the entire system or its individual links, or its suppression, causing the development of an immunodeficiency state. The quantitative determination of proteins of the immunoglobulin superfamily, namely, natural antibodies and cell receptors, provides valuable clinical information for clarifying the pathogenesis of the disease and differential diagnosis. The immunoglobulin profile of the body determines the functional ability of the immune system to recognize foreign agents. B-cell membrane immunoglobulins are the receptor for the antigen and are identical to its secreted form - the antibody. The modulation of their number reflects the processes of activation and inhibition of cellular reactions. The cell is constantly recycling membrane proteins. Similar processes occur with immunoglobulins, some of which are immersed in the cell, while others, on the contrary, are built into the membrane or secreted into the extracellular space in soluble form. Immunodiagnostics based on the determination of the immunoglobulins that make up the membrane and soluble pools makes it possible to assess the functional state of not only B cells, but also the immunoreactivity of the body as a whole. This paper presents a quantitative assessment of the soluble and membrane forms of clinically healthy cattle immunoglobulins using poly- and monoclonal antibodies.
The effect of nanoparticles of biogenic ferrihydrite on the Lemna minor L. growth
Authors: Subbotin M A , Muchkina E Ya , Tskhay I I , Krayanova T A
Published: January 2020

The article presents the data of experimental studies to identify the effect of biogenic cobalt-doped ferrihydrite nanoparticles on the growth of a model test plant Lemna minor. The test object was exposed for 7 days in a Steinberg medium with the addition of nanoparticles suspension (1.5 mg/l, 4.5 mg/l, and 15.0 mg/l). The analysis of the experimental data showed a suppressive effect of biogenic cobalt-doped ferrihydrite nanoparticles on the number of fronds and the growth rate of Lemna minor at a concentration of 15.0 mg/l. The indicators of root growth (number, length, weight) were similar to the control ones. The obtained data confirm the available data about the negative impact of elements in the form of nanomaterials at high concentrations and require further studies.
Influence of unconventional mineral complexes on the biochemical and hematological parameters of the blood of broiler chickens
Authors: Tyurina L E , Tabakov N A , Lefler T F , Turitsyna E G
Published: January 2020

The research results of the measurements of biochemical and hematological blood parameters of broiler chickens of the cross “Ross 308” depending on the inclusion of mineral additives based on industrial wastes into diet are presented in this article. The researches were conducted in 2017 at the premises of LLC “YeniseiAgroSoyuz” of Sukhobuzimsky district of Krasnoyarsk region and the stock-raising farm of the Institute of Applied Biotechnology and Veterinary Medicine of Krasnoyarsk State Agrarian University of the Ministry of Agriculture of Russia. The purpose of the research was to justify the use of mineral sources of Krasnoyarsk region as a part of animal feed for poultry. It has been established that the use of belite sludge and oxidized brown coal is one of the promising areas of non-waste technology for processing and mining of alumina and brown coal in the territory of Krasnoyarsk region.
Ecological and technological quotas anthropogenic load in basin of the Velikaya River
Authors: Antonov I V , Epifanov A V
Published: January 2020

An algorithm is proposed for environmental and technological standardization anthropogenic load on water bodies basins. An indicator has been developed to assess the level of environmental friendliness of technologies used in production. This indicator, proposed for early levels of environmental impact and the subsequent distribution of permissible loads between enterprises it is within the basins of the normative norm of admissible influence. This approach is implemented in the distribution of permissible load within the water basin of the Velikaya River.
Assessment of cows of Yenisei intra-breed type of red-mottled breed on a complex of signs
Authors: Alekseeva E A , Eremina I Yu
Published: January 2020

The article is devoted to a comprehensive study of cows of the Yenisei inbreeding type of red-mottled breed. The paper studies the level of milk productivity in groups with different values of the production typicality coefficient and the relationship between the genotype and production typicality coefficient. It was established that the coefficient of production typicality can be used to predict productivity, optimize selection and create a model group of cows. The data obtained allowed us to determine the basic requirements for cows of the desired production types: average milk yield of 6487 ± 2020 kg of milk per 305 days of lactation in first-calf heifers and 7891 ± 419 kg in cows of the third lactation and older; live weight - for first-calf heifers 543-574 kg, adults - 616-684 kg. The formation of the herd should be carried out by animals of the desired production types, which will contribute to the creation of highly productive livestock and increase the efficiency of selection and breeding work to improve breeding and productive qualities. When creating a group of animals of the desired types, including for genomic selection, it is possible not to take into account the origin of the animals.
Biotechnological potential of new strains of lactic acid bacteria
Authors: Kitaevskaya S V , Ponomarev V Y , Hasanova A F , Romanova N K
Published: January 2020

The paper presents data on the ferment effect of acid bacteria on model protein substrates in order to determine their proteolytic potential for biotransformation of fresh meat. The proteolytic activity of new starter cultures of lactic acid bacteria was evaluated. Strains of lactic acid bacteria Lbm. casei and Lbm. curvatus, exhibiting high proteolytic activity, can be recommended for inclusion in the composition of starter cultures.
Evaluation of long-term pasture chemical composition and productivity
Authors: Baikalova L P , Gorbachev I A , Yedimeichev Yu F , Mashanov A I , Smolin S G , Tabakov N A
Published: January 2020

The assessment of perennial legume-grass plants for pasture purposes was carried out on the basis of data from field experience conducted in the forest-steppe of Krasnoyarsk region and data from a qualitative analysis of pasture grass mixtures. The content of macronutrients: calcium, phosphorus, magnesium; green mass yield, collection of dry matter and feed units in three grazing cycles were determined. The chemical composition and productivity of pastures were depended on the type of grass, the cycle of grazing and the composition of the mixture. It was noted an increase in the calcium content in grass mixtures by 3.2-3.9 times to the control of red fescue. The phosphorus content in pasture feeds was at the control level. A higher content of magnesium in comparison with the control was found in the herbal mixture of brome + cat’s-tail grass + red fescue + goat’s-rue + clover. A grass mixture of brome + cat’s tail grass + red fescue + lucerne was the best in terms of yield of green mass, collection of dry matter and feed units.
Polyazolidinammonium as an adjuvant in immunization with lipopolysaccharide of Yersinia pseudotuberculosis
Authors: Kuznetsova V S , Ivaschenko S V , Domnitsky I Y
Published: January 2020

The use of polyazolidinammonium modified with iodine hydrate ions (PAAG) as an adjuvant made it possible to obtain rabbit hyperimmune serums for the pseudotuberculosis microbe lipopolysaccharide (LPS) with specific specificity. The optimal immunizing dose for obtaining hyperimmune pseudotuberculous sera was a dose of 0.25 mg of LPS Yersinia pseudotuberculosis O:3 serovariant per rabbit. When immunizing rabbits with LPS of the pseudotuberculosis microbe, PAAG showed more pronounced adjuvant properties compared to Freund’s complete adjuvant.
Morphometric indexes of a wall of arterial vessels of various bodies at animals
Authors: Kochetova O V , Kostarev S N , Sidorova K A , Ermolina S A , Sereda T G
Published: January 2020

The Chlamydia infection of farm animals remains quite significant problem in veterinary science and medicine now. At clamidiosis the permeability the gistogematicheskikh of barriers is broken that leads to degenerative changes of cages of an organism and respectively to development of a symptomatology in animals (The basic building block the gistogematicheskikh of barriers should be considered a capillary wall, to be exact the basal membrane which is a base line of the attack through which there pass substances from blood in body tissue). Endothelium cages in blood vessels form the semipermeable barrier between contents of vessels and the fabrics surrounding them. At healthy animals endothelial cells densely adjoin to each other and poorly interact with the cages circulating in blood. At a chlamydial infection we established localization of the activator in a wall of capillaries that leads to a hypertrophy of endothelial cells. As a result of this process a part of endotheliocytes is exfoliated in a gleam of vessels, and collapsing, promotes generalization of an infection on an organism.
The results of interspecific potato hybrids evaluation using marker-assisted, laboratory and field methods
Authors: Biryukova V A , Churakov A A , Zharova V A , Khalipsky A N , Kozlov V A
Published: January 2020

The evaluation of interspecific potato hybrids was carried out in 2018–2019 in Moscow region and Krasnoyarsk region. Using the methods of traditional and marker-assisted selection the interspecific hybrids created at the Scientific and Practical Center for Potato and Horticulture in Belarus were screened. According to the results of molecular genetic analysis, forms with complex resistance to a potato cyst nematode, Y, X viruses were identified: 110xy 0911-19, 89y 06-2a, 38dy-39d, 71-10-10, 212-216-9, KC 211xy 04 -10, 18-06-2, 54-10-3. As sources of economic characteristics, interest in practical selection is represented by samples with a high starch content in different ecological zones: 201.161-11, 213.24-31, 60-10-6, 53-10-5, 2011 14-8; pollen fertility: 201.206-48, 201.13-11, 001125-43; resistance to Phytophthora infestans : 89y 06-2a, 18-06-2, 106y 07-22, 209.3-1, 201.13-11, 60-10-6, 54-10-3 etc.; high marketable yield, mass ofgrocery-ware tuber: 106y07-22, 201.13-11, 110xy 0911-19, 89y 06-2a, 38dy-39d; favorable chemical composition for processing on potato products, chips, fries: 213.24-31, 213.11-32, 201.13-11, 89y 06-2a, 18ay 10-2.
Clostridium spp. detection in food samples using 16S rDNA-based PCR method
Authors: Bataeva D S , Makhova A A , Krylova V B , Gustova T V , Minaev M Yu
Published: January 2020

Raw foods of animal and plant origin are the most likely to be contaminated with either spores or vegetative cells of Clostridium. Molecular detection of the Clostridium spp. by polymerase chain reaction (PCR) is one useful method for detecting Clostridium spp. in some samples such as food raw materials. Clostridium detection was performed using 16S rDNA-based PCR method with Clos 58 - f and Clos 780 - r primers. A study of the specificity of the primers used revealed a reaction to Salmonella and Proteus. Therefore, the scope of PCR with these primers can be food products that have undergone high-temperature processing, for example, sterilized meat or meat and vegetable preserves, as well as ingredients after inactivation of vegetative forms of microorganisms.
Comparative evaluation of Yersinia dimethyl-sulfoxide antigens and antibodies obtained from it
Authors: Manieson V E , Ivashchenko S V
Published: January 2020

A comparative assessment of the antigenic properties of dimethyl-sulfoxide fractions (DA) of Yersinia pseudotuberculosis and Yersinia enterocolitica, as well as antibodies to them. DA Yersinia pseudotuberculosis has been shown to have greater antigenic activity than DA Yersinia enterocolitica. Hyperimmune serum obtained for pseudotuberculous DA showed generic yersiniosis specificity and high antibody activity, which allows its use in diagnostic test systems.
Morphophysiological features of spermatozoa of male white rats under experimental stress
Authors: Arestova I Yu , Sharonova E G , Kupriyanova M Yu
Published: January 2020

The results of research on the influence of stressful factors on the morphological parameters of spermatozoa are presented. A series of experiments was carried out using sexually mature outbred white rats (90 days of life). The experimental group of animals was subjected to combined stress (three days - hunger, after the third day - emotional stress, in accordance with The Forced Swim Test). The sperm count per unit volume of the epididymal suspension was calculated, activity was assessed under a microscope, and morphology was studied. The negative effect of stress factors on the number, motility and morphology of spermatozoa has been identified.
Features of free radical oxidation of lipids in cows with fat liver dystrophy
Authors: Abramov A A , Dolgov E P , Yakusheva L I , Kuzminova E V , Semenenko M P , Kovaluk N V , Semenenko K A
Published: January 2020

The mechanisms of the pathogenesis of liver damages of various genesis include destabilization of hepatocyte membranes, depending on the initiation of lipid peroxidation. The article presents re-search to determine the characteristics of lipid peroxidation in cows with fatty liver dystrophy. It was found out that in highly productive Holstein cows with a diagnosis of fatty hepatosis, the pro-cesses of free radical lipid oxidation are activated with an increase in the concentration of all lipoperoxidation products (diene conjugates, ketodienes and malondialdehyde) in blood and milk. Moreover, in sick animals, the level of antioxidant defense indicators such as carotene, vitamin C and glutathione peroxidase decreases.
Cross-resistance in animals with early postnatal cold imprinting
Authors: Selyatitskaya V G , Palchikova N A , Kuzminova O I
Published: January 2020

The article describes the cross-resistance to cold, hypoxia and physical activity of adult animals with early postnatal cold imprinting. It was shown that short-term fractional cooling in the first seven days of the life of Wistar rats increased the resistance of adult animals to low temperatures, but reduced it to hypoxia and physical static performance.
Advanced seedless donors among grape varieties of the Anapa zonal experimental station for grape growing and wine making (AZESGGAWM) selection
Authors: Gorbunov I V , Lukyanova A A , Lukyanov A A , Akhmedova Y A
Published: January 2020

The share of seedless grape varieties in the total grape production in the country is very small. Of the 106 table varieties included in the register, only 4 are seedless grape varieties (Kishmish Luchistiy, Korinka Russkaya, Yuzhnoberezhniy, Yaltinskiy Bessemyanniy). These varieties are very much in demand, as their berries are used both fresh and dried. This article shows the results of multi-year research on the identification of seedless genetic donors among grape varieties of the selection of the Anapa Zonal Experimental Station for Grape Growing and Wine Making, such as Lotos, Zhemchug Anapy and Kishmish Rozovy AZOS. In the frame of this study, agronomic characters and annually changing weather conditions were studied side by side. In addition, an assessment of these varieties was carried out in terms of the intensity of the seedless character in the conditions of the Anapa-Taman grape growing zone. The largest mass of rudiments in all the analyzed samples was found in the Kishmish Rozovy AZOS variety; therefore, it was assigned to the third category of seedless grape, although the ratio of the rudiment mass to the berry mass is negligible. The rest of the studied seedless grape varieties in comparison with the control variety (Kishmish Luchistiy) have a higher seedless class. In particular, this concerns the varieties – Zhemchug Anapy and Lotos belonging to the first and second seedless class, respectively. At the same time, yield of these varieties and their productivity (fruit-bearing and fruitfulness coefficients) together with plant development phenology depending on weather conditions were studied for several years. In particular, it was found that annually high productivity indicators are observed in all the studied varieties, as well as high yields are observed every year in the varieties Lotos and Zhemchug Anapy. This article presents the results of these studies from 2017 to 2019. The work performed showed the prospect of using productive, resistant to biotic and abiotic environmental factors with a high seedless category of grape varieties of the AZESGGaWM selection as future seedless donors.
Morphofunctional changes in the uterine tissues of laboratory animals in chlamydial infection
Authors: Tatarnikova N A
Published: January 2020

In diseases caused by Chl psittaci, a hematogenic type of infection is often observed, accompanied by damage to the CNS and internal organs: liver, spleen, lung. Lymphogenic spread of chlamydia (Chl trachomatis, venereal lymphogranulematosis) is possible. The hematogenic pathway of infection is most frequently observed in Chl trachomatis diseases (serovars D and K). Different biological variants (subspecies) of chlamydia cause lesions of different organs and systems. Chl trachomatis causes conjunctivitis, locally affects the mucous eye and urogenital tract.
Improving the efficiency of technological measurements when monitoring production and environmental indicators of thermal power plants
Authors: Tynchenko Ya A , Kovalev I V
Published: January 2020

The approaches are considered that provide an increase in the efficiency of technological measurements when monitoring production and environmental indicators of thermal power plants. The experience of expert evaluation of gas analytical systems is analyzed taking into account the technological parameters of equipment used in production processes at thermal power plants. The need for expert assessment is justified by the fact that currently there are no universal (generalized) formulas expressing the dependence of production and environmental parameters on each other or on the parameters of the technological process at thermal power plants. As practice shows, such dependencies are determined experimentally and most often with the involvement of specialists from commissioning organizations.
Desertification assessment of the territory of Atyrau region
Authors: Yessenamanova M S , Yessenamanova Zh S , Tlepbergenova A E , Abdinov R Sh , Ryskalieva D K
Published: January 2020

The article assesses the dynamics of desertification based on changes in the area of moving sands in the territories of the villages Issatay, Zineden, Maykomgen, Miyaly Zhaskairat over the past 17 years based on the processed data of remote sensing satellite images. It should be noted that the research area is located almost on the border of the semi-desert and desert zones, which within Kazakhstan occupy 57.7% of the territory. Ecosystems of these arid territories are very vulnerable to impacts, especially in modern conditions of climate change, reduction of water resources and active anthropogenic development. It should be noted that land degradation has a serious socio-economic impact on the standard of living and health of the population, especially on socially vulnerable sections of the rural population.
Rational use of wheat processing products to create a composite flour mixture
Authors: Gubanenko G A , Naimushina L V , Zhukova K O , Rechkina A , Mayurnikova L A , Kiseleva O V
Published: January 2020

The article presents data on the chemical composition of the powder from sprouted wheat pomace, which determines its high nutritional value: dietary fiber (45%), vitamins, mg / 100g (C - 450, P-active substances 390), minor components, mg / 100g (flavonoids 690, chlorophyll 41, tannins 1310) and minerals, mg / 100g (calcium 60, magnesium 85, phosphorus 300, iron 6.8). The resulting powder is able to provide a daily physiological need for minor components from 41 to 1950%, for micro and macromineral substances from 6 to 48%. It also strengthens the functional significance of the main components in the composition of enriched products due to biologically active substances and dietary fiber. Titrimetric, photometric, and spectrophotometric standardized methods were used in the study of the powder chemical composition and the quality indicators of the composite flour mixture. The composition is justified. A rational ratio of wheat, hemp flour, and powder from sprouted wheat pomace - 70:20:10 respectively is determined, based on the results of organoleptic and physico-chemical indicators. The study revealed increased nutritional value compared to wheat flour. The developed composite flour mixture is able to meet 40 % of physiological norm in terms of nutrients and more than 5000% in selenium; it can be considered as an alternative to wheat flour for the development of enriched food products.
Simulation of agricultural landscape catenas based on the analysis of hydrodynamic characteristics of the underlying surfaces of slope lands
Authors: Vasiliev S A
Published: January 2020

The agricultural landscape of slope lands is characterized by ecological instability and to ensure its equilibrium state a system of agronomic, melioration and environmental measures is carried out. Assessing the state of slope agricultural landscapes, it is necessary to take into account slope parameters, contour sizes, hydrological regime, types and varieties of soils, erosion processes, economic conditions, location, etc. Monitoring the above parameters will allow to detect hidden impacts or changes and restore the stability of the agricultural ecosystem early, for example, to maintain the hydrodynamic characteristics of the underlying surface for a certain period. Comprehensive research including methods of mathematical simulation and analysis carried out on the agricultural landscape catena is most effective. One of the fundamental scientific problems to be solved in this is to study and construct agricultural landscape catenas using the analysis of hydrodynamic characteristics of soil and vegetation cover of slope lands. It has been established that for compensatory melioration measures and engineering melioration systems planned on agricultural landscapes of slope lands, the following water flow rates occur: from 0.022 to 1.5 m/s for phytomelioration measures when slopes are rather steep; from 0.138 m/s for agricultural melioration measures at a slope of 0.08; and for traditional tillage rates exceed the critical ones and reach 0.8 m/s. According to the nomogram for determining the velocity diagram on a particular catchment slope, it is possible to assess and control anti-erosion melioration measures, revealing the actual speed regime of water flow in problem areas, and, therefore, it is possible to prevent erosion processes and preserve soil fertility on meliorated slope lands early.
Comparative evaluation of seeding spring wheat methods when using different types of coulters
Authors: Zaitsev A M , Solodun V I , Gorbunova M S
Published: January 2020

The studies were carried out with the aim of identifying the most effective method for sowing spring wheat for the Pre-Baikal region through a comparative study of sowing machines of domestic and foreign production with different types of openers according to the backgrounds of dump, non-dump and zero tillage. The research was carried out on the basis of advanced farms in the Irkutsk region, widely using modern sowing units in 2016-2018 on typical gray forest loamy soils. In experiments, in comparison with the SZ-3,6 seeder, the Case IH Concord 2000, John Deere 1830, Morris Concept 2000, Tom-10 sowing complexes were studied, providing strip-spread, tape, single-line sowing with paw, anchor and disk coulters. As a result, it was found that the application of the band-spread method of sowing with the Concord 2000, John Deere 1830, Morris Concept 2000 sowing complexes increases the field germination of spring wheat seeds by 10-20%, plant standing density by 34-42%, and yield by 0.38- 0.47 t/ha. An increase in the depth of seed placement to 5-6 cm led to an increase in the yield of wheat by 0.16 t/ha when sowing SZ-3.6; 0.26 t/ha when sowing John Deere 1830; 0.23 t/ha when sowing Concord 2000. In the pre-Baikal agricultural sector, to increase the yield of grain crops, it is advisable instead of the ordinary method of sowing with the SZ-3.6 seeders to apply the tape and strip-spread methods of sowing with the paw and anchor coulters both for dump and for subsurface processing and for direct sowing. The most promising sowing machines are the sowing complexes Concord 2000, John Deere 1830, Morris Concept 2000 and their domestic counterparts Kuzbass-8.5.
Rapeseed productivity in mixed crops of Tuva
Authors: Zhulanova V N , Ulyanova O A , Rylova O V , Saaya Ch S
Published: January 2020

In the steppe zone, the productivity of spring rape for green fodder was studied in single-species and poly-species crops with annual bluegrass and leguminous crops. It was established that single-species crops have high productivity; but they are characterized by low adaptive ability. Thus, their productivity in the steppe zone of Tuva depends on weather conditions. It was determined that in the crops of spring rape with peas, reliable increases in the yield of green mass were obtained. The economic efficiency of spring rape cultivation in two-component crops on the territory of Tuva republic is determined.
Fluorescence procedures to assess the photosynthetic resilience in Scots pines after a surface fire
Authors: Gette I G , Pakharkova N V
Published: January 2020

Forest fire represents one of the most serious abiotic stress factors that influence the function and productivity of ecosystems globally. Siberian pine forests are often exposed to forest fires but they are not always harmful to them. We have analyzed the effects of repeated heat stress on the photosynthetic apparatus of Scotch pines before and after surface fire exposure that occurred in the growing season in 2014. The survey area was the forest steppe zone of Krasnoyarsk region (South Siberia). First, we investigated the changes in the temperature-dependent responses of photosynthesis in the needles by means of fast and delayed chlorophyll fluorescence. Our results are indicative of some acclimation of Scotch pines after fire to the repeated high temperature stress. Also, this paper discusses the possibility of using chlorophyll fluorescence parameters for evaluating the presence of physiological changes after the fire affects. The time after fire effect and growth season were found to be important to assess the repair of photosynthesis and pigment content.
Estimation of the time dependence of fires quantity in Russia
Authors: Kalach A V , Kaybichev I A , Tararykin A M , Porkhachev M Yu , Shcherbin A N , Oblienko A V
Published: January 2020

Using correlation analysis of the statistical data for 2001-2016, the fact of strong dependence between the number of fires in the regions of the Russian Federation and the number per year was established. It has been established that Kabardino-Balkaria republic is an exception to this dependence. A hypothesis test of the relevance of the correlation coefficient found that the correlation coefficient between the number of fires and the number of years was 0.99.
Changes in physico-chemical and biological properties of rocks during weathering and soil formation
Authors: Dolgopolova N V , Batrachenko E A
Published: January 2020

Currently great attention is paid around the world to the problem of protecting the biosphere and the rational use of biological resources. The issues of protection of the atmosphere, water sources, flora and fauna, soil cover are becoming increasingly acute. Research is underway to develop the most rational principles for organizing industrial production that exclude environmental pollution. Particularly noteworthy are the issues of protection and rational use of soil cover. In the entire history of the development of human society, it has lost 20 million km2 of land resources. Currently, the loss of land on the planet for agricultural use is 5-7 million hectares annually. These lands go for building settlements on them, laying roads, mining, are lost due to erosion and salinization, the creation of industrial complexes, reservoirs, etc. Scientists estimate that the world’s population is growing by 68–70 million people every year, population growth and its increase is associated with the solution of major national and international problems, and this is the focus of the work.