bluuebunny/crossref_metadata_2025 huggingface dataset:
Chl1, an ATP-Dependent DNA Helicase, Inhibits DNA:RNA Hybrids Formation at DSB Sites to Maintain Genome Stability in S. pombe
Authors: He Deyun , Du Zhen , Xu Huiling , Bao Xiaoming
Published: June 2022

As an ATP-dependent DNA helicase, human ChlR1/DDX11 (Chl1 in yeast) can unwind both DNA:RNA and DNA:DNA substrates in vitro. Studies have demonstrated that ChlR1 plays a vital role in preserving genome stability by participating in DNA repair and sister chromatid cohesion, whereas the ways in which the biochemical features of ChlR1 function in DNA metabolism are not well understood. Here, we illustrate that Chl1 localizes to double-strand DNA break (DSB) sites and restrains DNA:RNA hybrid accumulation at these loci. Mutation of Chl1 strongly impairs DSB repair capacity by homologous recombination (HR) and nonhomologous end-joining (NHEJ) pathways, and deleting RNase H further reduces DNA repair efficiency, which indicates that the enzymatic activities of Chl1 are needed in Schizosaccharomyces pombe. In addition, we found that the Rpc37 subunit of RNA polymerase III (RNA Pol III) interacts directly with Chl1 and that deletion of Chl1 has no influence on the localization of Rpc37 at DSB site, implying the role of Rpc37 in the recruitment of Chl1 to this site.
DNA Demethylation Induces Tree Peony Flowering with a Low Deformity Rate Compared to Gibberellin by Inducing PsFT Expression under Forcing Culture Conditions
Authors: Sun Kairong , Xue Yuqian , Prijic Zeljana , Wang Shunli , Markovic Tatjana , Tian Caihuan , Wang Yingying , Xue Jingqi , Zhang Xiuxin
Published: June 2022

Gibberellin (GA) is frequently used in tree peony forcing culture, but inappropriate application often causes flower deformity. Here, 5-azacytidine (5-azaC), an efficient DNA demethylating reagent, induced tree peony flowering with a low deformity rate by rapidly inducing PsFT expression, whereas GA treatment affected various flowering pathway genes with strong pleiotropy. The 5-azaC treatment, but not GA, significantly reduced the methylation level in the PsFT promoter with the demethylation of five CG contexts in a 369 bp CG-rich region, and eight light-responsive related cis-elements were also predicted in this region, accompanied by enhanced leaf photosynthetic efficiency. Through GO analysis, all methylation-closer differentially expressed genes (DEGs) were located in the thylakoid, the main site for photosynthesis, and were mainly involved in response to stimulus and single-organism process, whereas GA-closer DEGs had a wider distribution inside and outside of cells, associated with 12 categories of processes and regulations. We further mapped five candidate DEGs with potential flowering regulation, including three kinases (SnRK1, WAK2, and 5PTase7) and two bioactive enzymes (cytochrome P450 and SBH1). In summary, 5-azaC and GA may have individual roles in inducing tree peony flowering, and 5-azaC could be a preferable regulation approach; DNA demethylation is suggested to be more focused on flowering regulation with PsFT playing a core role through promoter demethylation. In addition, 5-azaC may partially undertake or replace the light-signal function, combined with other factors, such as SnRK1, in regulating flowering. This work provides new ideas for improving tree peony forcing culture technology.
Approaches to Formaldehyde Measurement: From Liquid Biological Samples to Cells and Organisms
Authors: Lipskerov Fedor A. , Sheshukova Ekaterina V. , Komarova Tatiana V.
Published: June 2022

Formaldehyde (FA) is the simplest aldehyde present both in the environment and in living organisms. FA is an extremely reactive compound capable of protein crosslinking and DNA damage. For a long time, FA was considered a “biochemical waste” and a by-product of normal cellular metabolism, but in recent decades the picture has changed. As a result, the need arose for novel instruments and approaches to monitor and measure not only environmental FA in water, cosmetics, and household products, but also in food, beverages and biological samples including cells and even organisms. Despite numerous protocols being developed for in vitro and in cellulo FA assessment, many of them have remained at the “proof-of-concept” stage. We analyze the suitability of different methods developed for non-biological objects, and present an overview of the recently developed approaches, including chemically-synthesized probes and genetically encoded FA-sensors for in cellulo and in vivo FA monitoring. We also discuss the prospects of classical methods such as chromatography and spectrophotometry, and how they have been adapted in response to the demand for precise, selective and highly sensitive evaluation of FA concentration fluctuations in biological samples. The main objectives of this review is to summarize data on the main approaches for FA content measurement in liquid biological samples, pointing out the advantages and disadvantages of each method; to report the progress in development of novel molecules suitable for application in living systems; and, finally, to discuss genetically encoded FA-sensors based on existing natural biological FA-responsive elements.
Interaction between Melatonin and NO: Action Mechanisms, Main Targets, and Putative Roles of the Emerging Molecule NOmela
Authors: Martínez-Lorente Sara E. , Pardo-Hernández Miriam , Martí-Guillén José M. , López-Delacalle María , Rivero Rosa M.
Published: June 2022

Melatonin (MEL), a ubiquitous indolamine molecule, has gained interest in the last few decades due to its regulatory role in plant metabolism. Likewise, nitric oxide (NO), a gasotransmitter, can also affect plant molecular pathways due to its function as a signaling molecule. Both MEL and NO can interact at multiple levels under abiotic stress, starting with their own biosynthetic pathways and inducing a particular signaling response in plants. Moreover, their interaction can result in the formation of NOmela, a very recently discovered nitrosated form of MEL with promising roles in plant physiology. This review summarizes the role of NO and MEL molecules during plant development and fruit ripening, as well as their interactions. Due to the impact of climate-change-related abiotic stresses on agriculture, this review also focuses on the role of these molecules in mediating abiotic stress tolerance and the main mechanisms by which they operate, from the upregulation of the entire antioxidant defense system to the post-translational modifications (PTMs) of important molecules. Their individual interaction and crosstalk with phytohormones and H2S are also discussed. Finally, we introduce and summarize the little information available about NOmela, an emerging and still very unknown molecule, but that seems to have a stronger potential than MEL and NO separately in mediating plant stress response.
Zebrafish Larvae Behavior Models as a Tool for Drug Screenings and Pre-Clinical Trials: A Review
Authors: Rosa João Gabriel Santos , Lima Carla , Lopes-Ferreira Monica
Published: June 2022

To discover new molecules or review the biological activity and toxicity of therapeutic substances, drug development, and research relies on robust biological systems to obtain reliable results. Phenotype-based screenings can transpose the organism’s compensatory pathways by adopting multi-target strategies for treating complex diseases, and zebrafish emerged as an important model for biomedical research and drug screenings. Zebrafish’s clear correlation between neuro-anatomical and physiological features and behavior is very similar to that verified in mammals, enabling the construction of reliable and relevant experimental models for neurological disorders research. Zebrafish presents highly conserved physiological pathways that are found in higher vertebrates, including mammals, along with a robust behavioral repertoire. Moreover, it is very sensitive to pharmacological/environmental manipulations, and these behavioral phenotypes are detected in both larvae and adults. These advantages align with the 3Rs concept and qualify the zebrafish as a powerful tool for drug screenings and pre-clinical trials. This review highlights important behavioral domains studied in zebrafish larvae and their neurotransmitter systems and summarizes currently used techniques to evaluate and quantify zebrafish larvae behavior in laboratory studies.
Mesenchymal Stem/Progenitor Cells and Their Derivates in Tissue Regeneration
Authors: Klimczak Aleksandra
Published: June 2022

Mesenchymal stem/stromal cells (MSC) have been extensively studied over the last 30 years in the context of their regenerative and immunomodulatory activities for potential application in regenerative medicine [...]
Profiling the Effect of Targeting Wild Isocitrate Dehydrogenase 1 (IDH1) on the Cellular Metabolome of Leukemic Cells
Authors: Shait Mohammed Mohammed Razeeth , Alzahrani Faisal , Hosawi Salman , Choudhry Hani , Khan Mohammad Imran
Published: June 2022

Leukemia is one of the most common primary malignancies of the hematologic system in both children and adults and remains a largely incurable or relapsing disease. The elucidation of disease subtypes based on mutational profiling has not improved clinical outcomes. IDH1/2 are critical enzymes of the TCA cycle that produces α-ketoglutarate (αKG). However, their mutated version is well reported in various cancer types, including leukemia, which produces D-2 hydroxyglutarate (D-2HG), an oncometabolite. Recently, some studies have shown that wild-type IDH1 is highly expressed in non-small cell lung carcinoma (NSCLC), primary glioblastomas (GBM), and several hematological malignancies and is correlated with disease progression. This work shows that the treatment of wild-type IDH1 leukemia cells with a specific IDH1 inhibitor shifted leukemic cells toward glycolysis from the oxidative phosphorylation (OXPHOS) phenotype. We also noticed a reduction in αKG in treated cells, possibly suggesting the inhibition of IDH1 enzymatic activity. Furthermore, we found that IDH1 inhibition reduced the metabolites related to one-carbon metabolism, which is essential for maintaining global methylation in leukemic cells. Finally, we observed that metabolic alteration in IDH1 inhibitor-treated leukemic cells promoted reactive oxygen species (ROS) formation and the loss of mitochondrial membrane potential, leading to apoptosis in leukemic cells. We showed that targeting wild-type IDH1 leukemic cells promotes metabolic alterations that can be exploited for combination therapies for a better outcome.
A Methodology to Sequence the Service Management Processes in IT Departments: Application to the Tourism Industry
Authors: Rubio Sánchez Juan Luis , García Revilla Mercedes Raquel , Martínez Moure Olga
Published: June 2022

One of the key elements to consider in business management is the management of IT (Information Technology) departments by implementing processes as described in the ITIL (Information Technology Infrastructure Library) standard. This is particularly important in industries that are not directly related to ICT (Information and Communication Technologies), such as the tourism industry. In this paper, we present a methodology to sequence the implementation of the ITIL processes in any company to support its development. The methodology is based on an optimization algorithm and the information extracted from a survey. The optimal sequence is built from this information and from the information of the restrictions imposed by the company that implements the ITIL processes. We applied the methodology to a company in the tourism industry. The sequence obtained shows the need to implement operative processes (initial positions in the sequence), and afterwards strategic processes (final positions in the sequence). A comparison with other authors’ proposals shows differences in the order of processes proposed by this methodology. The main conclusion is that it is possible to use the information of companies to efficiently generate an optimal sequence of ITIL processes which enhances their management; this sequence is unique for every company that wants to implement ITIL in the tourism industry, and it is independent from the proposals of other authors who designed generic/non-optimal sequences.
Tetraruthenium Macrocycles with Laterally Extended Bis(alkenyl)quinoxaline Ligands and Their F4TCNQ•− Salts
Authors: Das Rajorshi , Linseis Michael , Senft Laura , Ivanović-Burmazović Ivana , Winter Rainer F.
Published: June 2022

We report on the tetraruthenium macrocycles Ru4-5 and -6 with a π-conjugated pyrene-appended 5,8-divinylquinoxaline ligand and either isophthalate or thiophenyl-2,5-dicarboxylate linkers and their charge-transfer salts formed by oxidation with two equivalents of F4TCNQ. Both macrocyclic complexes were characterized by NMR spectroscopy, mass spectrometry, cyclic and square-wave voltammetry, and by IR, UV–vis–NIR, and EPR spectroscopy in their various oxidation states.
Double-Balloon Kyphoplasty Results in Better Radiographic Outcomes Than a Single-Balloon Kyphoplasty in Treating Osteo-Porotic Spinal Fractures
Authors: Lotan Raphael , Haimovich Yaron , Schorr Louis , Goldstein Adam Lee , Hershkovich Oded
Published: June 2022

Background: Studies have found that unilateral and bilateral kyphoplasty have comparable clinical outcomes. Only a few studies have compared the radiographic results of using unilateral vs. simultaneous bilateral approaches. We aimed to examine and compare the radiographic results of unilateral (UKP) vs. bilateral simultaneous double-balloon kyphoplasty (DKP) for treating symptomatic vertebral compression fractures (VCF). Methods: A retrospective cohort of all patients treated for VCF by DKP and UKP over five years in a single medical center. From 2009 to 2012, we routinely performed UKP; from 2012, DKP was the routine due to potential benefits in vertebral realignment. We evaluated pre- and post-surgical fracture characteristics including vertebral height, sagittal and coronal Cobb angle, and fracture reduction. Statistical analysis included a t-test for independent variables and Pearson’s correlation. Results: The study cohort consisted of 81 patients (75.8 years ± 10.86) who underwent surgery, with a total of 119 vertebras. We performed 89 UKP on fractured vertebras and 30 DKP on 30 vertebrae. The UKP average fluoroscopy radiation exposure was 15.8 mGy (±11.5) per level compared to 11.2 mGy (±8.7) for DKP, p = 0.03. DKP showed significant fracture reduction, 2.8 degrees of Cobb angle, equaling the patient positioning effect on fracture reduction. Conclusion: DKP results in better fracture reduction than UKP, and equals the effect of patient positioning without increased radiation exposure or adverse events.
Is There a Difference between Perineural Dexamethasone with Single-Shot Interscalene Block (SSIB) and Interscalene Indwelling Catheter Analgesia (IICA) for Early Pain after Arthroscopic Rotator Cuff Repair? A Pilot Study
Authors: Kim Yang-Soo , Park Youngkyung , Koh Hyun Jung
Published: June 2022

Interscalene block is applied to control acute postoperative pain after arthroscopic rotator cuff repair (ARCR), typically with single-shot interscalene block (SSIB) or continuous interscalene indwelling catheter analgesia (IICA), and dexamethasone (Dex) for extending the analgesic effect. This study investigated whether perineural Dex can extend the postoperative analgesic effect of SSIB to match that of IICA. A total of 130 patients were recruited and divided into two groups (Group D, SSIB with perineural Dex, n = 94; Group C, IICA, n = 36). The surgical and anesthetic processes were identical except for the method of nerve block. Pain was measured by a visual analog scale (VAS) at 6, 12, 24, and 48 h after ARCR. The number of each and the total analgesics used and adverse effects were compared. The duration of ARCR was longer in group D. The VAS score was higher in group C 6 h after ARCR, but there was no difference at other time points. More postoperative analgesics were administered to group C, and there was no difference in the number of adverse effects. In conclusion, combining perineural Dex with SSIB can reduce rebound hyperalgesia after 6 h and extend the duration of the analgesic effect to that of IICA. Therefore, IICA could be substituted with SSIB and Dex between at 6 and 48 h after ARCR.
Influence of Probiotics in Prevention and Treatment of Patients Who Undergo Chemotherapy or/and Radiotherapy and Suffer from Mucositis, Diarrhoea, Constipation, Nausea and Vomiting
Authors: Garczyk Aleksandra , Kaliciak Iwona , Drogowski Konstanty , Horwat Paulina , Kopeć Stanisław , Staręga Zuzanna , Bogdański Paweł , Stelmach-Mardas Marta , Mardas Marcin
Published: June 2022

The administration of probiotics to patients treated with chemo- and/or radiotherapy is assumed to be beneficial. The aim of this study was to evaluate the effects of probiotic intake on the severity of selected gastrointestinal side effects of chemotherapy and radiotherapy. The searched databases included PubMed, Web of Science, and Scopus from which twenty-one studies were included. Most of them concerned diarrhoea, however, two of the studies examined constipation, another two nausea and vomiting, and eight of the included studies regarded mucositis. The total number of patients equalled 2621. The time of the conducted therapy, the administered species, neoplasm pathology, and adjuvant therapy varied. The outcome was assessed by gathering information about the statistical significance of the improvements. An enhancement was observed in thirteen studies, where probiotics had a significant impact on each of the included chemo- and/or radiotherapy side effects. However, the heterogeneity of the assessed data makes it impossible to state a firm conclusion.
Efficacy of Photobiomodulation in Reducing Symptomatology and Improving the Quality of Life in Patients with Xerostomia and Hyposalivation: A Randomized Controlled Trial
Authors: Ferrandez-Pujante Alba , Pons-Fuster Eduardo , López-Jornet Pia
Published: June 2022

Objectives: To evaluate the efficacy of photobiomodulation (PBM) treatment in patients with xerostomia and hyposalivation and assess their quality of life over a one year of follow-up. Material and methods: A single-blind randomized controlled trial. A total of 60 patients with xerostomia were included (30 PBM cases and 30 placebo controls). Photobiomodulation was performed with a diode laser (810 nm, 6 J/cm2), while the controls underwent simulated treatment. One weekly session was carried out for a total of 6 weeks (total six sessions). The study parameters were recorded at baseline, after three and six weeks, and at one year post-treatment. Xerostomia was assessed using a salivary flow visual analog scale (VAS) and the Xerostomia Inventory (XI). The Hospital Anxiety and Depression (HAD) scale, Oral Health Impact Profile (OHIP-14), Pittsburgh Sleep Quality Index (PSQI) and Epworth Sleepiness Scale (ESS) were also administered. Results: The patients subjected to PBM therapy showed a significant improvement of xerostomia based on the drainage test, and of oral quality of life (p < 0.001). The depression score of the HAD (HAD-D) and the ESS showed improvement, though without reaching statistical significance (p > 0.05). The placebo group showed significant changes in the xerostomia VAS score at 6 weeks (p = 0.009), with no variations in any of the other studied parameters (p > 0.05). The beneficial effects of the diode laser in the PBM group persisted at one year post-treatment. Conclusions: Photobiomodulation with the diode laser is effective in patients with xerostomia and hyposalivation, and thus should be taken into account as a treatment option.
Central Symptoms of Insomnia in Relation to Depression and COVID-19 Anxiety in General Population: A Network Analysis
Authors: Cha Eun Jung , Jeon Hong Jun , Chung Seockhoon
Published: June 2022

Background: Insomnia is prevalent among the general population, and studies have shown an increase in insomnia symptoms during the novel coronavirus (COVID-19) pandemic. Despite numerous studies of insomnia, few studies have investigated insomnia symptoms in detail. In this study, we used network analysis to investigate interactions between insomnia symptoms in the general population. Furthermore, given the effect of COVID-19 on mental health, we also investigated how anxiety response to COVID-19 and depression related to insomnia symptoms. Methods: Data from 785 non-infected participants were used. The Insomnia Severity Index (ISI), Stress and Anxiety to Viral Epidemics—6 Scale (SAVE-6), and Patient Health Questionnaire—9 (PHQ-9) were used to measure insomnia symptoms, anxiety response to COVID-19, and depression, respectively. Network analysis was performed using R Studio. Centrality indices and edge weights were obtained, and each index was evaluated using bootstrapping methods. Results: The network revealed ISI7 (worry about current sleep pattern) to be the most central insomnia symptom. ISI7 was strongly connected to SAVE-6 total score, and ISI2 (difficulty staying asleep) was strongly connected to PHQ-9 total score. Conclusion: High centrality of ISI7 supports the role of dysfunctional cognitions in etiological models of insomnia and thus the cognitive behavioral therapy for insomnia. The relationship between ISI7 and SAVE-6 is explained by transposition of worry and fear of contracting COVID-19 to worry about sleep patterns. The link between ISI2 and PHQ-9 necessitate further investigations of whether specific symptoms of insomnia are more associated with depression.
Impaired Antibody Response Is Associated with Histone-Release, Organ Dysfunction and Mortality in Critically Ill COVID-19 Patients
Authors: Lagedal Rickard , Eriksson Oskar , Sörman Anna , Huckriede Joram B. , Kristensen Bjarne , Franzén Stephanie , Larsson Anders , Bergqvist Anders , Alving Kjell , Forslund Anders , Persson Barbro , Ekdahl Kristina N. , Garcia de Frutos Pablo , Nilsson Bo , Nicolaes Gerry A. F. , Lipcsey Miklos , Hultström Michael , Frithiof Robert
Published: June 2022

Purpose: the pathophysiologic mechanisms explaining differences in clinical outcomes following COVID-19 are not completely described. This study aims to investigate antibody responses in critically ill patients with COVID-19 in relation to inflammation, organ failure and 30-day survival. Methods: All patients with PCR-verified COVID-19 and gave consent, and who were admitted to a tertiary Intensive care unit (ICU) in Sweden during March–September 2020 were included. Demography, repeated blood samples and measures of organ function were collected. Analyses of anti-SARS-CoV-2 antibodies (IgM, IgA and IgG) in plasma were performed and correlated to patient outcome and biomarkers of inflammation and organ failure. Results: A total of 115 patients (median age 62 years, 77% male) were included prospectively. All patients developed severe respiratory dysfunction, and 59% were treated with invasive ventilation. Thirty-day mortality was 22.6% for all included patients. Patients negative for any anti-SARS-CoV-2 antibody in plasma during ICU admission had higher 30-day mortality compared to patients positive for antibodies. Patients positive for IgM had more ICU-, ventilator-, renal replacement therapy- and vasoactive medication-free days. IgA antibody concentrations correlated negatively with both SAPS3 and maximal SOFA-score and IgM-levels correlated negatively with SAPS3. Patients with antibody levels below the detection limit had higher plasma levels of extracellular histones on day 1 and elevated levels of kidney and cardiac biomarkers, but showed no signs of increased inflammation, complement activation or cytokine release. After adjusting for age, positive IgM and IgG antibodies were still associated with increased 30-day survival, with odds ratio (OR) 7.1 (1.5–34.4) and 4.2 (1.1–15.7), respectively. Conclusion: In patients with severe COVID-19 requiring intensive care, a poor antibody response is associated with organ failure, systemic histone release and increased 30-day mortality.
Impact of Continuous Flow Left Ventricular Assist Device on Heart Transplant Candidates: A Multi-State Survival Analysis
Authors: Carrozzini Massimiliano , Bottio Tomaso , Caraffa Raphael , Bejko Jonida , Bifulco Olimpia , Guariento Alvise , Lombardi Carlo Mario , Metra Marco , Azzolina Danila , Gregori Dario , Fedrigo Marny , Castellani Chiara , Tarzia Vincenzo , Toscano Giuseppe , Gambino Antonio , Jorgji Vjola , Ferrari Enrico , Angelini Annalisa , Gerosa Gino
Published: June 2022

(1) Objectives: The aim of this study was to investigate the impact of the prolonged use of continuous-flow left ventricular assist devices (LVADs) on heart transplant (HTx) candidates. (2) Methods: Between January 2012 and December 2019, we included all consecutive patients diagnosed with end-stage heart failure considered for HTx at our institution, who were also eligible for LVAD therapy as a bridge to transplant (BTT). Patients were divided into two groups: those who received an LVAD as BTT (LVAD group) and those who were listed without durable support (No-LVAD group). (3) Results: A total of 250 patients were analyzed. Of these, 70 patients (28%) were directly implanted with an LVAD as BTT, 11 (4.4%) received delayed LVAD implantation, and 169 (67%) were never assisted with an implantable device. The mean follow-up time was 36 ± 29 months. In the multivariate analysis of survival before HTx, LVAD implantation showed a protective effect: LVAD vs. No-LVAD HR 0.01 (p < 0.01) and LVAD vs. LVAD delayed HR 0.13 (p = 0.02). Mortality and adverse events after HTx were similar between LVAD and No-LVAD (p = 0.65 and p = 0.39, respectively). The multi-state survival analysis showed a significantly higher probability of death for No-LVAD vs. LVAD patients with (p = 0.03) or without (p = 0.04) HTx. (4) Conclusions: The use of LVAD as a bridge to transplant was associated with an overall survival benefit, compared to patients listed without LVAD support.
Dependence of the Dynamic Mechanical Properties and Structure of Polyurethane-Clay Nanocomposites on the Weight Fraction of Clay
Authors: Peng Shirley , Iroh Jude O.
Published: June 2022

The effect of clay and chemical cross-linking on the dynamic mechanical properties of polyurethane reinforced with different concentrations of organically modified montmorillonite clay is investigated in this study. The polyurethane matrix is constituted of polytetrahydrofuran soft segment and 4,4′-methylenebis(phenyl isocyanate) hard segment. Glycerin was used as the chemical crosslinking agent, while Cloisite 30B clay was the reinforcing filler. The nanocomposites containing up to 1 wt.% clay showed a uniform dispersion of clay; however, the nanocomposites containing higher concentrations of clay showed the presence of heterogeneities. Dynamic mechanical spectroscopy, DMS revealed that the nanocomposites containing between 2 and 10 wt.% clay had two glass transition temperatures, Tg,1 and Tg,2. The higher-temperature glass transition temperature, Tg,2 increased with increasing clay concentration, while the low-temperature glass transition temperature, Tg,1 decreased with increasing clay concentration. The nanocomposites containing low clay concentrations up to 1 wt.% showed only one glass transition temperature with a narrow glass transition region. The crosslink density for the nanocomposites increased with increasing wt.% clay.
Crucial Convolution: Genetic and Molecular Mechanisms of Coiling during Epididymis Formation and Development in Embryogenesis
Authors: Wong Joanne , Gasperoni Jemma , Fuller Jarrad , Grommen Sylvia V. H. , De Groef Bert , Hogarth Cathryn , Dworkin Sebastian
Published: June 2022

As embryonic development proceeds, numerous organs need to coil, bend or fold in order to establish their final shape. Generally, this occurs so as to maximise the surface area for absorption or secretory functions (e.g., in the small and large intestines, kidney or epididymis); however, mechanisms of bending and shaping also occur in other structures, notably the midbrain–hindbrain boundary in some teleost fish models such as zebrafish. In this review, we will examine known genetic and molecular factors that operate to pattern complex, coiled structures, with a primary focus on the epididymis as an excellent model organ to examine coiling. We will also discuss genetic mechanisms involving coiling in the seminiferous tubules and intestine to establish the final form and function of these coiled structures in the mature organism.
Additive Manufacturing: An Opportunity for the Fabrication of Near-Net-Shape NiTi Implants
Authors: Safavi Mir Saman , Bordbar-Khiabani Aydin , Khalil-Allafi Jafar , Mozafari Masoud , Visai Livia
Published: June 2022

Nickel–titanium (NiTi) is a shape-memory alloy, a type of material whose name is derived from its ability to recover its original shape upon heating to a certain temperature. NiTi falls under the umbrella of metallic materials, offering high superelasticity, acceptable corrosion resistance, a relatively low elastic modulus, and desirable biocompatibility. There are several challenges regarding the processing and machinability of NiTi, originating from its high ductility and reactivity. Additive manufacturing (AM), commonly known as 3D printing, is a promising candidate for solving problems in the fabrication of near-net-shape NiTi biomaterials with controlled porosity. Powder-bed fusion and directed energy deposition are AM approaches employed to produce synthetic NiTi implants. A short summary of the principles and the pros and cons of these approaches is provided. The influence of the operating parameters, which can change the microstructural features, including the porosity content and orientation of the crystals, on the mechanical properties is addressed. Surface-modification techniques are recommended for suppressing the Ni ion leaching from the surface of AM-fabricated NiTi, which is a technical challenge faced by the long-term in vivo application of NiTi.
A Quantitative Parametric Study on Output Time Delays for Autonomous Underwater Cleaning Operations
Authors: Sørensen Fredrik Fogh , von Benzon Malte , Liniger Jesper , Pedersen Simon
Published: June 2022

Offshore pipelines and structures require regular marine growth removal and inspection to ensure structural integrity. These operations are typically carried out by Remotely Operated Vehicles (ROVs) and demand reliable and accurate feedback signals for operating the ROVs efficiently under harsh offshore conditions. This study investigates and quantifies how sensor delays impact the expected control performance without the need for defining the control parameters. Input-output (IO) controllability analysis of the open-loop system is applied to find the lower bound of the H-infinity peaks of the unspecified optimal closed-loop systems. The performance analyses have shown that near-structure operations, such as pipeline inspection or cleaning, in which small error tolerances are required, have a small threshold for the time delays. The IO controllability analysis indicates that off-structure navigation allow substantial larger time delays. Especially heading is vulnerable to time delay; however, fast-responding sensors usually measure this motion. Lastly, a sensor comparison is presented where available sensors are evaluated for each ROV motion’s respective sensor-induced time delays. It is concluded that even though off-structure navigation have larger time delay tolerance the corresponding sensors also introduce substantially larger time delays.
Environmental Assessment of Potentially Toxic Elements Using Pollution Indices and Data-Driven Modeling in Surface Sediment of the Littoral Shelf of the Mediterranean Sea Coast and Gamasa Estuary, Egypt
Authors: El-Safa Magda M. Abou , Elsayed Salah , Elsherbiny Osama , Elmetwalli Adel H. , Gad Mohamed , Moghanm Farahat S. , Eid Ebrahem M. , Taher Mostafa A. , El-Morsy Mohamed H. E. , Osman Hanan E. M. , Saleh Ali H.
Published: June 2022

Coastal environmental assessment techniques have evolved into one of the most important fields for the long-term development and management of coastal zones. So, the overall aim of the present investigation was to provide effective approaches for making informed decisions about the Gamasa coast sediment quality. Over a two-year investigation, sediment samples were meticulously collected from the Gamasa estuary and littoral shelf. The inductively coupled plasma mass spectra (ICP-MS) was used to the total concentrations of Al, Fe, Ti, Mg, Mn, Cu, P, V, Ba, Cr, Sr, Co, Ni, Zn, Pb, Zr, and Ce. Single elements environmental pollution indices including the geoaccumulation index (Igeo), contamination factor (CF), and enrichment factor (EF), as well as multi-elements pollution indices comprising the potential ecological risk index (RI), degree of contamination (Dc), and pollution load index (PLI) were used to assess the sediment and the various geo-environmental variables affecting the Mediterranean coastal system. Furthermore, the Dc, PLI, and RI were estimated using the random forest (RF) and Back-Propagation Neural Network (BPNN) depending on the selected elements. According to the Dc results, all the investigated sediment samples categories were considerably contaminated. Cr, Co, Ni, Cu, Zr, V, Zn, P, and Mn showed remarkable enrichment in sediment samples and were originated from anthropogenic sources based on the CF, EF, and Igeo data. Moreover, the RI findings revealed that all the samples tested pose a low ecologically risk. Meanwhile, based on PLI, 70% of the Gamasa estuary samples were polluted, while 93.75% of littoral shelf sediment was unpolluted. The BPNNs -PCs-CD-17 model performed the best and demonstrated a better association between exceptional qualities and CD. With R2 values of 1.00 for calibration (Cal.) and 1.00 for validation (Val.). The BPNNs -PCs-PLI-17 models performed the best in terms of measuring PLI with respective R2 values of 1.00 and 0.98 for the Cal. and Val. datasets. The findings showed that the RF and BPNN models may be used to precisely quantify the pollution indices (Dc, PLI, and RI) in calibration (Cal.) and validation (Val.) datasets utilizing potentially toxic elements of surface sediment.
Perceived Qualities, Visitation and Felt Benefits of Preferred Nature Spaces during the COVID-19 Pandemic in Australia: A Nationally-Representative Cross-Sectional Study of 2940 Adults
Authors: Feng Xiaoqi , Astell-Burt Thomas
Published: June 2022

We investigated how the perceived quality of natural spaces influenced levels of visitation and felt benefits during the COVID-19 pandemic in Australia via a nationally representative online and telephone survey conducted on 12–26 October (Social Research Centre’s Life in AustraliaTM panel aged > 18 years, 78.8% response, n = 3043). Our sample was restricted to those with complete information (n = 2940). Likert scale responses to 18 statements regarding the quality of local natural spaces that participants preferred to visit were classified into eight quality domains: access; aesthetics; amenities; facilities; incivilities; potential usage; safety; and social. These domains were then summed into an overall nature quality score (mean = 5.8, range = 0–16). Associations between these quality variables and a range of nature visitation and felt benefits were tested using weighted multilevel models, adjusted for demographic and socioeconomic confounders. Compared with participants in the lowest perceived nature quality quintile, those in the highest quality quintile had higher odds of spending at least 2 h in their preferred local nature space in the past week (Odds Ratio [OR] = 3.40; 95% Confidence Interval [95%CI] = 2.38–4.86), of visiting their preferred nature space almost every day in the past four weeks (OR = 3.90; 2.77–5.47), and of reporting increased levels of nature visitation in comparison with before the COVID-19 pandemic (OR = 3.90; 2.54–6.00). Participants in the highest versus lowest perceived nature quality quintile also reported higher odds of feeling their visits to nature enabled them to take solace and respite during the pandemic (OR = 9.49; 6.73–13.39), to keep connected with their communities (OR = 5.30; 3.46–8.11), and to exercise more often than they did before the pandemic (OR = 3.88; 2.57–5.86). Further analyses of each quality domain indicated time in and frequency of visiting nature spaces were most affected by potential usage and safety (time in nature was also influenced by the level of amenity). Feelings of connection and solace were most affected by potential usage and social domains. Exercise was most influenced by potential usage, social and access domains. In conclusion, evidence reported in this study indicates that visits to nature and various health-related benefits associated with it during the COVID-19 pandemic were highly contingent upon numerous qualities of green and blue spaces.
Mitigation of Deicing Salt Loading to Water Resources by Transpiration from Green Infrastructure Vegetation
Authors: Zhang Wuhuan , Burgis Charles R. , Hayes Gail M. , Henderson Derek A. , Smith James A.
Published: June 2022

Green infrastructure (GI) protects aquatic ecosystems from stormwater runoff caused by urban development. Bioretention (BR) is a typical GI system wherein stormwater runoff is routed to a soil basin planted with vegetation and has been shown to reduce deicing salt loads in surface runoff, but the removal mechanism of salt is poorly understood. This study explores the potential of different vegetation types to reduce deicing salt released from a BR by transpiration. Six engineered soil media columns were built in a laboratory greenhouse to simulate a 1012 m2 BR basin along Lorton Road, Fairfax County, VA, USA. The effect of vegetation type (Blue Wild Indigo and Broadleaf Cattail) and influent salt concentration on flow volume and salt mass reduction were quantified for multiple storm events. For all storm events, chloride inflow concentrations, and vegetation types, Cl− load reduction ranged from 26.1% to 33.5%, Na+ load reduction ranged from 38.2% to 47.4%, and volume reductions ranged from 11.4% to 41.9%. Different inflow salt concentrations yielded different removal rates of deicing salt, and for a given column, salt removal decreased over sequential storm events. For each influent salt concentration, columns planted with Broadleaf Cattail (BC) performed better for volume and salt mass reductions than columns planted with Blue Wild Indigo (BWI), which in turn performed better than the controls.
cAMP Is a Promising Regulatory Molecule for Plant Adaptation to Heat Stress
Authors: Liang Shuang , Sun Jinfeng , Luo Yanmin , Lv Shanshan , Chen Jiajia , Liu Yanpei , Hu Xiuli
Published: June 2022

With gradual warming or increased frequency and magnitude of high temperature, heat stress adversely affects plant growth and eventually reduces plant productivity and quality. Plants have evolved complex mechanisms to sense and respond to heat stress which are crucial to avoiding cell damage and maintaining cellular homeostasis. Recently, 33″,55″-cyclic adenosine monophosphate (cAMP) has been proved to be an important signaling molecule participating in plant adaptation to heat stress by affecting multi-level regulatory networks. Significant progress has been made on many fronts of cAMP research, particularly in understanding the downstream signaling events that culminate in the activation of stress-responsive genes, mRNA translation initiation, vesicle trafficking, the ubiquitin-proteasome system, autophagy, HSPs-assisted protein processing, and cellular ion homeostasis to prevent heat-related damage and to preserve cellular and metabolic functions. In this present review, we summarize recent works on the genetic and molecular mechanisms of cAMP in plant response to heat stress which could be useful in finding thermotolerant key genes to develop heat stress-resistant varieties and that have the potential for utilizing cAMP as a chemical regulator to improve plant thermotolerance. New directions for future studies on cAMP are discussed.
Hypofractionation and Concomitant Boost in Ductal Carcinoma In Situ (DCIS): Analysis of a Prospective Case Series with Long-Term Follow-Up
Authors: Cante Domenico , Paolini Marina , Piva Cristina , Petrucci Edoardo , Radici Lorenzo , Ferrario Silvia , Mondini Guido , Bagnera Silvia , La Porta Maria Rosa , Franco Pierfrancesco
Published: June 2022

We previously reported on a cohort of breast cancer patients affected with ductal carcinoma in situ (DCIS) that were treated with breast conservative surgery and hypofractionated whole-breast radiotherapy with a concomitant boost to the lumpectomy cavity. We now report on the long-term results of the oncological and toxicity outcomes, at a median follow-up of 11.2 years. We also include an analysis of the predictive factors for local recurrence (LR). Eighty-two patients with long-term observation were considered for this report. All received hypofractionated post-operative radiotherapy with a concomitant boost (45 Gy/20 fractions to the whole breast and 50 Gy/20 fractions to the lumpectomy cavity). We report on LC rates at 5 and 10 years, overall survival (OS), and breast-cancer-specific survival (BCSS), employing the Kaplan–Meier method. Cox proportional regression analysis was used to determine the role of selected clinical parameters on the risk of local recurrence, by the univariate and multivariate models. After a median follow-up of 11.2 years (range 5–15 years), 9 pts (11%) developed LR. The LR rates at 5 years and 10 years were 2.4% and 8.2%, respectively. The 5- and 10-year overall survival rates were 98.8% and 91.6%, respectively. The 5- and 10-year breast-cancer-specific survival rates were 100.0% and 99.0%. Late skin and subcutaneous toxicities were generally mild, and cosmetic results were good–excellent for most patients. For the univariate regression analysis, ER positive status (HR; 95% CI, p = 0.021), PgR positive status (HR; 95% CI, p = 0.012), and the aggregate data of positive hormonal status (HR; 95% CI, p = 0.021) were inversely correlated to LR risk. Conversely, a high tumor grade (G3) was directly correlated with the risk of LR (HR; 95% CI, p = 0.048). For the multivariate regression analysis, a high tumor grade (G3) confirmed its negative impact on LR (HR 0.40; 95% CI 0.19–0.75, p = 0.047). Our long-term data demonstrate hypofractionated whole-breast radiotherapy with a concomitant boost to be feasable, effective, and tolerable. Our experience suggests positive hormonal status to be protective with respect to LR risk. A high tumor grade is a risk factor for LR.
Compressive Strength of Steel Fiber-Reinforced Concrete Employing Supervised Machine Learning Techniques
Authors: Li Yongjian , Zhang Qizhi , Kamiński Paweł , Deifalla Ahmed Farouk , Sufian Muhammad , Dyczko Artur , Kahla Nabil Ben , Atig Miniar
Published: June 2022

Recently, research has centered on developing new approaches, such as supervised machine learning techniques, that can compute the mechanical characteristics of materials without investing much effort, time, or money in experimentation. To predict the 28-day compressive strength of steel fiber–reinforced concrete (SFRC), machine learning techniques, i.e., individual and ensemble models, were considered. For this study, two ensemble approaches (SVR AdaBoost and SVR bagging) and one individual technique (support vector regression (SVR)) were used. Coefficient of determination (R2), statistical assessment, and k-fold cross validation were carried out to scrutinize the efficiency of each approach used. In addition, a sensitivity technique was used to assess the influence of parameters on the prediction results. It was discovered that all of the approaches used performed better in terms of forecasting the outcomes. The SVR AdaBoost method was the most precise, with R2 = 0.96, as opposed to SVR bagging and support vector regression, which had R2 values of 0.87 and 0.81, respectively. Furthermore, based on the lowered error values (MAE = 4.4 MPa, RMSE = 8 MPa), statistical and k-fold cross validation tests verified the optimum performance of SVR AdaBoost. The forecast performance of the SVR bagging models, on the other hand, was equally satisfactory. In order to predict the mechanical characteristics of other construction materials, these ensemble machine learning approaches can be applied.
Casting Welding from Magnesium Alloy Using Filler Materials That Contain Scandium
Authors: Shalomeev Vadym , Tabunshchyk Galyna , Greshta Viktor , Korniejenko Kinga , Duarte Guigou Martin , Parzych Sławomir
Published: June 2022

Based on the results achieved in systematic studies of structure formation and the formation of multicomponent phases, a scandium-containing filler metal from system alloy Mg-Zr-Nd for welding of aircraft casting was developed. The influence of scandium in magnesium filler alloy on its mechanical and special properties, such as long-term strength at elevated temperatures, was studied by the authors. It is established that modification of the magnesium alloy with scandium in an amount between 0.05 and 0.07% allows a fine-grained structure to be obtained, which increases its plasticity up to 70% and heat resistance up to 1.8 times due to the formation of complex intermetallic phases and the microalloying of the solid solution. Welding of the aircraft castings made of magnesium alloy with scandium-containing filler material allows obtaining a weld with a dense homogeneous fusion zone and the surrounding area without any defects. The developed filler material for welding surface defects (cracks, chips, etc.) formed during operation on aircraft engine bodies makes it possible to restore cast body parts and reuse them. The proposed filler material composition with an improved set of properties for the welding of body castings from Mg-Zr-Nd system alloy for aircraft engines makes it possible to increase their reliability and durability in general, extend the service life of aircraft engines, and obtain a significant economic effect.