bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical Impact of COVID-19 on Multi-Drug-Resistant Gram-Negative Bacilli Bloodstream Infections in an Intensive Care Unit Setting: Two Pandemics Compared
Authors: Cogliati Dezza Francesco , Arcari Gabriele , Alessi Federica , Valeri Serena , Curtolo Ambrogio , Sacco Federica , Ceccarelli Giancarlo , Raponi Giammarco , Alessandri Francesco , Mastroianni Claudio Maria , Venditti Mario , Oliva Alessandra
Published: July 2022

Two mutually related pandemics are ongoing worldwide: the COVID-19 and antimicrobial resistance pandemics. This study aims to evaluate the impact of COVID-19 on multi-drug-resistant Gram-negative bacteria (MDR-GN) bloodstream infections (BSIs) in a single intensive care unit (ICU). We conducted a retrospective study including patients admitted to the ICU, reorganized for COVID-19 patients’ healthcare, with at least one confirmed MDR-GN BSI during 2019–2020. We compared clinical and microbiological features, incidence density, antibiotic therapy and mortality rate in pre- and during-COVID-19 pandemic periods. We estimated the impact of COVID-19 on mortality by means of univariate Cox regression analyses. A total of 46 patients were included in the study (28 non-COVID-19/18 COVID-19). Overall, 63 BSI episodes occurred (44/19), and non-COVID-19 patients had a higher incidence of MDR-GN BSIs and were more likely to present K. pneumoniae BSIs, while the COVID-19 group showed more A. baumannii BSIs with higher per pathogen incidence. COVID-19 patients presented more critical conditions at the BSI onset, a shorter hospitalization time from BSI to death and higher 30-day mortality rate from BSI onset. COVID-19 and septic shock were associated with 30-day mortality from MDR-GN BSIs, while early active therapy was a protective factor. In conclusion, COVID-19 showed a negative impact on patients with MDR-GN BSIs admitted to the ICU.
Methods of Isolation of Active Substances from Garlic (Allium sativum L.) and Its Impact on the Composition and Biological Properties of Garlic Extracts
Authors: Bar Monika , Binduga Urszula E. , Szychowski Konrad A.
Published: July 2022

Garlic (Allium sativum L.) is widely used in the human diet and in scientific research due to its biological properties. Various factors, e.g., temperature, pressure, extraction method, type of solvent, size, and territorial origin of garlic, affect the amount and type of bioactive compounds obtained from garlic extracts. In turn, the content of bioactive compounds correlates with the biological activity of the extracts. Therefore, the aim of this review was to summarize the current state of knowledge of the methods and effectiveness of isolation of active substances from garlic and their impact on the garlic extract composition and, consequently, biological properties. According to the literature, extracts obtained using water as a solvent are mainly responsible for antimicrobial properties, which is related to, inter alia, the high content of allicin. The use of alcohols, such as methanol or ethanol, is associated with the outstanding antioxidant power of extracts resulting from the presence of phenolic compounds. In turn, due to the presence of diallyl disulfide and disulfide trisulfide, garlic oil has anticancer potential. Acetone is the most effective organic solvent; however, it is not suitable for immediate consumption.
Anti-Inflammatory, Antioxidant, and Skin Regenerative Potential of Secondary Metabolites from Plants of the Brassicaceae Family: A Systematic Review of In Vitro and In Vivo Preclinical Evidence (Biological Activities Brassicaceae Skin Diseases)
Authors: Mattosinhos Patricia da Silva , Sarandy Mariáurea Matias , Novaes Rômulo Dias , Esposito Debora , Gonçalves Reggiani Vilela
Published: July 2022

The Brassicaceae family constitutes some of the most well-studied natural products in the world, due to their anti-inflammatory, anti-oxidative, and pro-regenerative properties as well as their ubiquitous distribution across the world. To evaluate the potential efficacy of the Brassicaceae family in the treatment of inflammatory skin disorders and wounds, based on preclinical evidence from in vivo and in vitro studies. This systematic review was performed according to the PRISMA guidelines, using a structured search on the PubMed-Medline, Scopus, and Web of Science platforms. The studies included were those that used murine models and in vitro studies to investigate the effect of Brassicaceae on skin disorders. Bias analysis and methodological quality assessments were examined through SYRCLE’s RoB tool. Brassicaceae have shown positive impacts on inflammatory regulation of the skin, accelerating the wound healing process, and inhibiting the development of edema. The studies showed that the Brassicaceae family has antioxidant activity and effects on the modulation of cyclooxygenase 2 and the nuclear factor kappa β (NFκβ) pathway. The secondary metabolites present in Brassicas are polyphenols (68.75%; n = 11), terpenes/carotenoids (31.25%; n = 5), and glycosylates (25%; n = 4), which are responsible for their anti-inflammatory, healing, and antioxidant effects. In addition, the current evidence is reliable because the bias analysis showed a low risk of bias. Our review indicates that compounds derived from Brassicaceae present exceptional potential to treat inflammatory skin diseases and accelerate cutaneous wound healing. We hope that our critical analysis can help to expedite clinical research and to reduce methodological bias, thereby improving the quality of evidence in future research. The registration number on the Prospero platform is CRD42021262953.
GPR and Digital Survey for the Diagnosis and the 3D Representation of the Battle of Issus Mosaic from the House of the Faun, Pompeii (Naples, Italy)
Authors: Cozzolino Marilena , De Simone Antonio , Gentile Vincenzo , Mauriello Paolo , Piezzo Amanda
Published: July 2022

The application of non-invasive geophysical techniques and digital surveys to explore cultural heritage is becoming a very important research field. The capability to detect inner and superficial changes in the inspected surfaces allows for imaging spatial inhomogeneity and material features and planning targeted conservation and restoration interventions. In this work, the results of a research project carried out on the famous Battle of Issus Mosaic, also known as the “Alexander Mosaic”, are presented. It is a masterpiece of ancient art that was found in 1831 in the House of Faun, the most luxurious and spacious house in Pompeii. It is notable for its size (3.41 × 5.82 m), the quality of workmanship and the subject that represents the culminating phase of the battle between Alexander Magno’s army and the Persian one of Darius. In 1916, it was moved inside the National Archaeological Museum of Naples, where the original horizontal location was changed with a vertical arrangement supported by an inner wooden structure, whose exact manufacture is unclear. Today, the mosaic is affected by important instability phenomena highlighted by the appearance of the significant detachment of tiles, superficial lesions and swelling of the surface. Given the important need to preserve it, a high-detail diagnostic study was realized through a digital survey and non-invasive geophysical surveys using ground-penetrating radar (GPR). The investigation was repeated after two years, in 2018 and 2020, with the aim of verifying the evolution of degradation. The work provided a high-resolution estimate of the state of the health of the mosaic and allowed for obtaining a three-dimensional reconstruction of the internal mosaic structure, including the formulation of hypotheses on the engineering supporting works of the twentieth century; this provides an essential tool for the imminent conservation project, which also implies restoring the original horizontal position.
Toward a Practical Digital Twin Platform Tailored to the Requirements of Industrial Energy Systems
Authors: Kasper Lukas , Birkelbach Felix , Schwarzmayr Paul , Steindl Gernot , Ramsauer Daniel , Hofmann René
Published: July 2022

Digitalization and concepts such as digital twins (DT) are expected to have huge potential to improve efficiency in industry, in particular, in the energy sector. Although the number and maturity of DT concepts is increasing, there is still no standardized framework available for the implementation of DTs for industrial energy systems (IES). On the one hand, most proposals focus on the conceptual side of components and leave most implementation details unaddressed. Specific implementations, on the other hand, rarely follow recognized reference architectures and standards. Furthermore, most related work on DTs is done in manufacturing, which differs from DTs in energy systems in various aspects, regarding, for example, multiple time-scales, strong nonlinearities and uncertainties. In the present work, we identify the most important requirements for DTs of IES. We propose a DT platform based on the five-dimensional DT modeling concept with a low level of abstraction that is tailored to the identified requirements. We address current technical implementation barriers and provide practical solutions for them. Our work should pave the way to standardized DT platforms and the efficient encapsulation of DT service engineering by domain experts. Thus, DTs could be easy to implement in various IES-related use cases, host any desired models and services, and help get the most out of the individual applications. This ultimately helps bridge the interdisciplinary gap between the latest research on DTs in the domain of computer science and industrial automation and the actual implementation and value creation in the traditional energy sector.
Sand: A Critical Component for Beach Volleyball Courts
Authors: Kasprzak Marek , Łopuch Michał
Published: July 2022

Beach volleyball is growing in popularity. However, the construction of beach sports courts away from the seashore requires the use of the right quality sand, the desired characteristics of which are defined by FIVB standards. We decided to test sand from 11 volleyball courts in Wrocław, Poland and sand from the Central Sport Centre-Olympic Preparation Centre (CSC-OPC) in Spała, the main training facility for Polish beach volleyball players. Laboratory tests showed that too coarse sand containing admixtures of dark minerals was used for most of the courts in Wrocław. The sand containing the properties most similar to FIVB standards is that at CSC-OPC. Even a small amount of silt found does not guarantee that a sandy surface will be dust-free. Silt can adhere to larger mineral grains and separate from them by mechanical impact or from crushing aggregates. In addition, we surveyed volleyball players. The players unequivocally interpreted the ease of play on finer-grained sands and the reduction of movement dynamics on courts with coarse-grained sand. Nevertheless, these are not the only factors determining their preference for their favourite court. Compliance with FIVB standards, however, guarantees comparable playing conditions.
Variability of the Chemical Composition of the Atmospheric Aerosol in the Coastal Zone of the Southern Basin of Lake Baikal (East Siberia, Russia)
Authors: Golobokova Liudmila P. , Khodzher Tamara V. , Zhamsueva Galina S. , Zayakhanov Alexander S. , Starikov Alexey , Khuriganova Olga I.
Published: July 2022

The role of the atmosphere in the formation of the chemical composition and quality of water in Lake Baikal and its tributaries has been increasing in recent years. In this regard, studies of the chemical composition of the constituents of the atmosphere have an important practical application. In 2020 and 2021, we studied the chemical composition of atmospheric aerosol, one of the indicators of air pollution, in the atmosphere of the coastal zone of the southern basin of Lake Baikal compared to the data from previous years. The studies were carried out in the summer on the southwestern (Bolshiye Koty) and southeastern coast (Boyarsky). In the absence of smoke in the aerosol on the southwest coast, the concentrations of NH4+, NO3− and SO42− ions prevailed. The mean total concentration of ions at the Bolshiye Koty research station was 2.08 ± 1.26 μg/m3. The appearance of smog contributed to the growth of the total ionic concentration in the aerosol on the southwest coast to 6.4 μg/m3 in 2020 and to 17.6 μg/m3 in 2021. On the southeast coast, the minimum concentration of the total amount of ions was 3.3 μg/m3. The concentrations of Ca2+, Na+, K+, Cl−, and SO42− ions prevailed in the aerosol. Under the influence of smog, the total amount of ions increased to 34.1 μg/m3 in 2020 and to 18.6 μg/m3 in 2021. In periods of intense smoke, NH4+ and SO42− became the dominant ions in the aerosols at both stations. The contribution of NO3− ions increased. Although the effect of natural factors is periodic, they contribute significantly to the change in the chemical composition of atmospheric aerosol.
A Review of the Methods of Non-Invasive Assessment of Intracranial Pressure through Ocular Measurement
Authors: Dong Jinhui , Li Qi , Wang Xiaofei , Fan Yubo
Published: July 2022

The monitoring of intracranial pressure (ICP) is essential for the detection and treatment of most craniocerebral diseases. Invasive methods are the most accurate approach to measure ICP; however, these methods are prone to complications and have a limited range of applications. Therefore, non-invasive ICP measurement is preferable in a range of scenarios. The current non-invasive ICP measurement methods comprise fluid dynamics, and ophthalmic, otic, electrophysiological, and other methods. This article reviews eight methods of non-invasive estimation of ICP from ocular measurements, namely optic nerve sheath diameter, flash visual evoked potentials, two-depth transorbital Doppler ultrasonography, central retinal venous pressure, optical coherence tomography, pupillometry, intraocular pressure measurement, and retinal arteriole and venule diameter ratio. We evaluated and presented the indications and main advantages and disadvantages of these methods. Although these methods cannot completely replace invasive measurement, for some specific situations and patients, non-invasive measurement of ICP still has great potential.
Effects of Surface-Type Plyometric Training on Physical Fitness in Schoolchildren of Both Sexes: A Randomized Controlled Intervention
Authors: Marzouki Hamza , Dridi Rached , Ouergui Ibrahim , Selmi Okba , Mbarki Rania , Klai Roudaina , Bouhlel Ezdine , Weiss Katja , Knechtle Beat
Published: July 2022

Plyometric training (PT) has been found to be effective for children’s fitness. However, no study has examined the effects of sex on physical fitness adaptations from surface-type PT in children. This study compared the effects of short-term surface-type PT (firm vs. sand) on the physical fitness of schoolchildren of both sexes. Sixty girls (age = 10.00 ± 1.15 years) and sixty boys (age = 10.02 ± 1.12 years) participated in a short-term (4 weeks), randomized and parallel PT design with pre-to-post measurements. Children were divided into two experimental groups (firm group: PT performed on a clay surface, 20 boys and 20 girls; sand group: PT performed on a dry surface of 20 cm deep sand, 20 boys and 20 girls) and a control group (CG, 20 boys and 20 girls). Squat jump, standing long jump, 20 m sprint, 5-10-5 shuttle, dynamic balance, and maximal aerobic velocity were measured at baseline and after intervention. Both experimental groups showed greater pre-post changes in all assessed variables than the CG (p < 0.0001). No significant differences in pre-post changes were observed relative to surface type or sex (p > 0.05). These findings suggest that a twice-weekly PT program induced physical fitness improvements, which may have transfer to health status during childhood. Additionally, surface type and sex did not affect the training-induced changes in physical fitness.
Culture Media Composition Influences the Antibacterial Effect of Silver, Cupric, and Zinc Ions against Pseudomonas aeruginosa
Authors: Rewak-Soroczynska Justyna , Dorotkiewicz-Jach Agata , Drulis-Kawa Zuzanna , Wiglusz Rafal J.
Published: July 2022

Different metals, such as silver (Ag), copper (Cu), and zinc (Zn), have been broadly investigated as metals and cations used both in medicine and everyday life due to their broad spectrum of antibacterial activity. Although the antibacterial action of those metals and their ions is well known and studied, the main problem remains in the standardization of experimental procedures to determine the antimicrobial activity as bacteriological media composition might significantly influence the outcome. The presented study aimed to evaluate the appropriability of different culture media (four nutritionally rich and four minimal) in the testing of the antibacterial activity of Ag+, Cu2+, and Zn2+ ions against Pseudomonas aeruginosa. Our investigation revealed the influence of medium ingredients and the presence of phosphates, which significantly reduced the activity of tested metal ions. Moreover, the precipitate formation and decrease in pH in the minimal media were additionally observed. It was assumed that the most favorable medium for metal ion activity testing was Luria-Bertani complex medium and MOPS minimal medium.
Fungicidal Activity of AP10W, a Short Peptide Derived from AP-2 Complex Subunit mu-A, In Vitro and In Vivo
Authors: Gong Yi , Li Haoyi , Wu Fei , Li Yishuai , Zhang Shicui
Published: July 2022

With the increase in the incidence of fungal infections, and the restrictions of existing antifungal drugs, the development of novel antifungal agents is urgent. Here we prove that AP10W, a short peptide derived from AP-2 complex subunit mu-A, displays conspicuous antifungal activities against the main fungal pathogens of human infections Candida albicans and Aspergillus fumigatus. We also show that AP10W suppresses the fungal biofilm formation, and reduces the pre-established fungal biofilms. AP10W appears to exert its fungicidal activity through a mode of combined actions, including interaction with the fungal cell walls via laminarin, mannan and chitin, enhancement of cell wall permeabilization, induction of membrane depolarization, and increase in intracellular ROS generation. Importantly, we demonstrate that AP10W exhibits little toxicity towards mammalian fibroblasts, and effectively promotes the healing of wounded skins infected by C. albicans. These together indicate that AP10W is a new member of fungicidal agents. It also suggests that AP10W has a considerable potential for future development as a novel antifungal drug.
Therapeutic Potential of Quercetin Loaded Nanoparticles: Novel Insights in Alleviating Colitis in an Experimental DSS Induced Colitis Model
Authors: Khater Safaa I. , Lotfy Marwa M. , Alandiyjany Maher N. , Alqahtani Leena S. , Zaglool Asmaa W. , Althobaiti Fayez , Ismail Tamer Ahmed , Soliman Mohamed Mohamed , Saad Saydat , Ibrahim Doaa
Published: July 2022

Oxidative stress is considered the main etiologic factor involved in inflammatory bowel disease (IBD). Integration of nanocarriers for natural therapeutic agents with antioxidant and anti-inflammatory potential is a novel promising candidate for curing IBD. Herein, the colonic antioxidant and anti-inflammatory effects of different concentrations of quercetin nanoparticles (QT-NPs) were evaluated using a dextran sulfate sodium (DSS)-induced colitis model. Following colitis induction, the efficacy and mechanistic actions of QT-NPs were evaluated by assessing lesion severity, molecular aids controlling oxidative stress and inflammatory response, and histopathological and immunohistochemistry examination of colonic tissues. Administration of QT-NPs, especially at higher concentrations, significantly reduced the disease activity index and values of fecal calprotectin marker compared to the colitic group. Colonic oxidant/antioxidant status (ROS, H2O2, MDA, SOD, CAT, GPX and TAC) was restored after treatment with higher concentrations of QT-NPs. Moreover, QT-NPs at levels of 20 mg/kg and, to a lesser extent, 15 mg/kg reduced Nrf2 and HO-1 gene expression, which was in line with decreasing the expression of iNOS and COX2 in colonic tissues. Higher concentrations of QT-NPs greatly downregulated pro-inflammatory cytokines; upregulated genes encoding occludin, MUC-2 and JAM; and restored the healthy architectures of colonic tissues. Taken together, these data suggest that QT-NPs could be a promising alternative to current IBD treatments.
Role of Erythropoietin Receptor Signaling in Macrophages or Choroidal Endothelial Cells in Choroidal Neovascularization
Authors: Ramshekar Aniket , Bretz Colin A. , Kunz Eric , Cung Thaonhi , Richards Burt T. , Stoddard Gregory J. , Hageman Gregory S. , Chaqour Brahim , Hartnett M. Elizabeth
Published: July 2022

Erythropoietin (EPO) has been proposed to reduce the progression of atrophic age-related macular degeneration (AMD) due to its potential role in neuroprotection. However, overactive EPO receptor (EPOR) signaling increased laser-induced choroidal neovascularization (CNV) and choroidal macrophage number in non-lasered mice, which raised the question of whether EPOR signaling increased CNV through the recruitment of macrophages to the choroid that released pro-angiogenic factors or through direct angiogenic effects on endothelial cells. In this study, we addressed the hypothesis that EPOR signaling increased CNV by direct effects on macrophages or endothelial cells. We used tamoxifen-inducible macrophage-specific or endothelial cell-specific EPOR knockout mice in the laser-induced CNV model, and cultured choroidal endothelial cells isolated from adult human donors. We found that macrophage-specific knockout of EPOR influenced laser-induced CNV in females only, whereas endothelial-specific knockout of EPOR reduced laser-induced CNV in male mice only. In cultured human choroidal endothelial cells, knockdown of EPOR reduced EPO-induced signal transducer and activator of transcription 3 (STAT3) activation. Taken together, our findings suggest that EPOR signaling in macrophages or choroidal endothelial cells regulates the development of CNV in a sex-dependent manner. Further studies regarding the role of EPO-induced signaling are required to assess EPO safety and to select or develop appropriate therapeutic approaches.
Comparing the Effects of Canagliflozin vs. Glimepiride by Body Mass Index in Patients with Type 2 Diabetes and Chronic Heart Failure: A Subanalysis of the CANDLE Trial
Authors: Sezai Akira , Tanaka Atsushi , Imai Takumi , Kida Keisuke , Sekino Hisakuni , Murohara Toyoaki , Sata Masataka , Suzuki Norio , Node Koichi
Published: July 2022

Background: We present results of a 24-week comparative study of the effects of the sodium–glucose cotransporter 2 (SGLT2) inhibitor canagliflozin vs. the sulfonylurea glimepiride, by baseline body mass index (BMI), in patients with type 2 diabetes and chronic heart failure. Methods: We conducted a post hoc analysis of the CANDLE trial. This subanalysis evaluated NT-proBNP, BMI, and other laboratory parameters, according to the subgroups stratified by BMI ≥ 25 kg/m2 vs. BMI < 25 kg/m2. Results: A group ratio of proportional changes in the geometric means of NT-proBNP was 0.99 (p = 0.940) for the subgroup with BMI ≥ 25 kg/m2 and 0.85 (p = 0.075) for the subgroup with BMI < 25 kg/m2, respectively. When baseline BMI was modeled as a continuous variable, results for patients with BMI < 30 kg/m2 showed a slightly smaller increase in NT-proBNP in the canagliflozin group vs. the glimepiride group (p = 0.295); that difference was not seen among patients with BMI ≥30 kg/m2 (p = 0.948). Irrespective of obesity, the canagliflozin group was associated with significant reduction in BMI compared to the glimepiride group. Conclusion: There was no significant difference in the effects of canagliflozin, relative to glimepiride, on NT-proBNP concentrations irrespective of baseline obesity. UMIN clinical trial registration number: UMIN000017669.
Classification of Dysphonic Voices in Parkinson’s Disease with Semi-Supervised Competitive Learning Algorithm
Authors: Bao Guidong , Lin Mengchen , Sang Xiaoqian , Hou Yangcan , Liu Yixuan , Wu Yunfeng
Published: July 2022

This article proposes a novel semi-supervised competitive learning (SSCL) algorithm for vocal pattern classifications in Parkinson’s disease (PD). The acoustic parameters of voice records were grouped into the families of jitter, shimmer, harmonic-to-noise, frequency, and nonlinear measures, respectively. The linear correlations were computed within each acoustic parameter family. According to the correlation matrix results, the jitter, shimmer, and harmonic-to-noise parameters presented as highly correlated in terms of Pearson’s correlation coefficients. Then, the principal component analysis (PCA) technique was implemented to eliminate the redundant dimensions of the acoustic parameters for each family. The Mann–Whitney–Wilcoxon hypothesis test was used to evaluate the significant difference of the PCA-projected features between the healthy subjects and PD patients. Eight dominant PCA-projected features were selected based on the eigenvalue threshold criterion and the statistical significance level (p < 0.05) of the hypothesis test. The SSCL algorithm proposed in this paper included the procedures of the competitive prototype seed selection, K-means optimization, and the nearest neighbor classifications. The pattern classification experimental results showed that the proposed SSCL method can provide the excellent diagnostic performances in terms of accuracy (0.838), recall (0.825), specificity (0.85), precision (0.846), F-score (0.835), Matthews correlation coefficient (0.675), area under the receiver operating characteristic curve (0.939), and Kappa coefficient (0.675), which were consistently better than those results of conventional KNN or SVM classifiers.
Full Soft Capacitive Omnidirectional Tactile Sensor Based on Micro-Spines Electrode and Hemispheric Dielectric Structure
Authors: Xu Baochun , Wang Yu , Cui Haoao , Niu Haoran , Liu Yijian , Li Zhongli , Chen Da
Published: July 2022

Flourishing in recent years, intelligent electronics is desirably pursued in many fields including bio-symbiotic, human physiology regulatory, robot operation, and human–computer interaction. To support this appealing vision, human-like tactile perception is urgently necessary for dexterous object manipulation. In particular, the real-time force perception with strength and orientation simultaneously is critical for intelligent electronic skin. However, it is still very challenging to achieve directional tactile sensing that has eminent properties, and at the same time, has the feasibility for scale expansion. Here, a fully soft capacitive omnidirectional tactile (ODT) sensor was developed based on the structure of MWCNTs coated stripe electrode and Ecoflex hemisphere array dielectric. The theoretical analysis of this structure was conducted for omnidirectional force detection by finite element simulation. Combined with the micro-spine and the hemispheric hills dielectric structure, this sensing structure could achieve omnidirectional detection with high sensitivity (0.306 ± 0.001 kPa−1 under 10 kPa) and a wide response range (2.55 Pa to 160 kPa). Moreover, to overcome the inherent disunity in flexible sensor units due to nano-materials and polymer, machine learning approaches were introduced as a prospective technical routing to recognize various loading angles and finally performed more than 99% recognition accuracy. The practical validity of the design was demonstrated by the detection of human motion, physiological activities, and gripping of a cup, which was evident to have great potential for tactile e-skin for digital medical and soft robotics.
TNFα-Induced Oxidative Stress and Mitochondrial Dysfunction Alter Hypothalamic Neurogenesis and Promote Appetite Versus Satiety Neuropeptide Expression in Mice
Authors: Desai Mina , Stiles Linsey , Torsoni Adriana S. , Torsoni Marcio A. , Shirihai Orian S. , Ross Michael G.
Published: July 2022

Maternal obesity results in programmed offspring hyperphagia and obesity. The increased offspring food intake is due in part to the preferential differentiation of hypothalamic neuroprogenitor cells (NPCs) to orexigenic (AgRP) vs. anorexigenic (POMC) neurons. The altered neurogenesis may involve hypothalamic bHLH (basic helix–loop–helix) neuroregulatory factors (Hes1, Mash1, and Ngn3). Whilst the underlying mechanism remains unclear, it is known that mitochondrial function is critical for neurogenesis and is impacted by proinflammatory cytokines such as TNFα. Obesity is associated with the activation of inflammation and oxidative stress pathways. In obese pregnancies, increased levels of TNFα are seen in maternal and cord blood, indicating increased fetal exposure. As TNFα influences neurogenesis and mitochondrial function, we tested the effects of TNFα and reactive oxidative species (ROS) hydrogen peroxide (H2O2) on hypothalamic NPC cultures from newborn mice. TNFα treatment impaired NPC mitochondrial function, increased ROS production and NPC proliferation, and decreased the protein expression of proneurogenic Mash1/Ngn3. Consistent with this, AgRP protein expression was increased and POMC was decreased. Notably, treatment with H2O2 produced similar effects as TNFα and also reduced the protein expression of antioxidant SIRT1. The inhibition of STAT3/NFκB prevented the effects of TNFα, suggesting that TNFα mediates its effects on NPCs via mitochondrial-induced oxidative stress that involves both signaling pathways.
Tolosa–Hunt Syndrome and Hemorrhagic Encephalitis Presenting in a Patient after COVID-19 Vaccination Followed by COVID-19 Infection
Authors: Gogu Anca Elena , Motoc Andrei Gheorghe , Docu Axelerad Any , Stroe Alina Zorina , Gogu Andreea Alexandra , Jianu Dragos Catalin
Published: July 2022

The presence of neurological symptoms within the clinical range of COVID-19 disease infection has increased. This paper presents the situation of a 45-year-old man having the medical antecedent diabetes mellitus, who presented to the emergency department with fever, headache, and respiratory symptoms, nine days following vaccination with the Ad26.COV2-S COVID-19 vaccine. The patient tested positive for SARS-CoV-2 based on nasal polymerase chain reaction (RT-PCR). Two weeks after the presentation, he developed Tolosa–Hunt Syndrome, an autoimmune phenomenon, with painful left ophthalmoplegia. Significant improvement was seen in terms of his discomfort; however, ptosis and ocular mobility improved only moderately after treatment with intravenous methylprednisolone, and the patient was discharged on a new insulin regimen. The patient returned after four weeks and the neurological exam results showed significant signs of right hemiparesis, mixed aphasia, incomplete left ophthalmoplegia, severe headache, and agitation; after a few days, the patient experienced a depressed level of consciousness and coma. The patient’s clinical condition worsened and, unfortunately, he died. MRI brain images revealed multiple ischemic strokes, meningitis, infectious vasculitis, and hemorrhagic encephalitis, which are all serious complications of COVID-19.
Feasibility of Canine Adenovirus Type 2 (CAV2) Based Vector for the Locus Coeruleus Optogenetic Activation in Non-Transgenic Rats: Implications for Functional Studies
Authors: Kabanova Anna , Cavani Elena , Logothetis Nikos K. , Eschenko Oxana
Published: July 2022

The locus coeruleus norepinephrine (LC-NE) system modulates many visceral and cognitive functions, while LC-NE dysfunction leads to neurological and neurodegenerative conditions such as sleep disorders, depression, ADHD, or Alzheimer’s disease. Innovative viral-vector and gene-engineering technology combined with the availability of cell-specific promoters enabled regional targeting and selective control over phenotypically specific populations of neurons. We transduced the LC-NE neurons in adult male rats by delivering the canine adenovirus type 2-based vector carrying the NE-specific promoter PRSx8 and a light-sensitive channelrhodopsin-2 receptor (ChR2) directly in the LC or retrogradely from the LC targets. The highest ChR2 expression level was achieved when the virus was delivered medially to the trigeminal pathway and ~100 μm lateral to the LC. The injections close or directly in the LC compromised the tissue integrity and NE cell phenotype. Retrograde labeling was more optimal given the transduction of projection-selective subpopulations. Our results highlight a limited inference of ChR2 expression from representative cases to the entire population of targeted cells. The actual fraction of manipulated neurons appears most essential for an adequate interpretation of the study outcome. The actual fraction of manipulated neurons appears most essential for an adequate interpretation of the study outcome. Thus, besides the cell-type specificity and the transduction efficiency, the between-subject variability in the proportion of the remaining viral-transduced targeted cell population must be considered in any functional connectivity study.
Time-Series Associations between Public Interest in COVID-19 Variants and National Vaccination Rate: A Google Trends Analysis
Authors: Cheng Cecilia
Published: July 2022

The emergence of a constantly mutating novel virus has led to considerable public anxiety amid the COVID-19 pandemic. Information seeking is a common strategy to cope with pandemic anxiety. Using Google Trends analysis, this study investigated public interest in COVID-19 variants and its temporal associations with the disease-prevention measure of vaccination during the initial COVID-19 vaccine rollout period (13 December 2020 to 25 September 2021). Public interest was operationalized as the relative search volume of online queries of variant-related terms in the countries first affected by the Alpha, Beta, and Delta variants: the UK, South Africa, and India, respectively. The results show that public interest in COVID-19 variants was greater during the Delta-variant-predominant period than before this period. The time-series cross-correlation analysis revealed positive temporal associations (i.e., greater such public interest was accompanied by an increase in national vaccination rate) tended to occur more frequently and at earlier time lags than the negative temporal associations. This study yielded new findings regarding the temporal changes in public interest in COVID-19 variants, and the between-country variations in these public interest changes can be explained by differences in the rate and pace of vaccination among the countries of interest.
Single-Particle Measurements of Nanocatalysis with Dark-Field Microscopy
Authors: Shang Jing , Fan Jinsong , Qin Weiwei , Li Kun
Published: July 2022

Due to the complexity of heterogeneous reactions and heterogeneities of individual catalyst particles in size, morphology, and the surrounding medium, it is very important to characterize the structure of nanocatalysts and measure the reaction process of nanocatalysis at the single-particle level. Traditional ensemble measurements, however, only provide averaged results of billions of nanoparticles (NPs), which do not help reveal structure–activity relationships and may overlook a few NPs with high activity. The advent of dark-field microscopy (DFM) combined with plasmonic resonance Rayleigh scattering (PRRS) spectroscopy provides a powerful means for directly recording the localized surface plasmon resonance (LSPR) spectrum of single plasmonic nanoparticles (PNPs), which also enables quantitative measurements. In recent years, DFM has developed rapidly for a series of single-particle catalytic reactions such as redox reactions, electrocatalytic reactions, and DNAzyme catalysis, with the ability to monitor the catalytic reaction process in real time and reveal the catalytic mechanism. This review provides a comprehensive overview of the fundamental principles and practical applications of DFM in measuring various kinds of catalysis (including chemocatalysis, electrocatalysis, photocatalysis, and biocatalysis) at the single-particle level. Perspectives on the remaining challenges and future trends in this field are also proposed.
Production of 1,3-Butadiene from Ethanol Using Treated Zr-Based Catalyst
Authors: Bojang Adama A. , Wu Ho-Shing
Published: July 2022

The conversion of ethanol to 1,3-butadiene was carried out using a treated Zr-based catalyst at a temperature of 350–400 °C with different weight hourly space velocities in a fixed bed reactor. The catalysts used are commercial, but they underwent pretreatment. The commercial catalysts used were ZrO2, Zr(OH)2, 2% CaO-ZrO2, 30% TiO2-ZrO2, 50% CeO2-ZrO2 and 10% SiO2-ZrO2 in their modified or treated form. The characterizations of the catalysts were carried out using XRD, XPS, and TGA. The results indicated that ethanol conversion, yield, and selectivity of 1,3-butadiene operated weight hourly space velocity of 2.5 h−1 using 10% SiO2-ZrO2 were 95%, 80%, and 85%, respectively, at 350 °C. Using 50% CeO2-ZrO2 converted 70% ethanol with a 1,3-butadiene yield of 65%. The best Zr-based catalyst was 10% SiO2-ZrO2 as it gives a steady 1,3-butadiene yield, the Si-composition with ZrO2 gives a good catalytic pour of the catalyst-bed structure; hence, the life span was good. Using 30% TiO2-ZrO2 has an ethanol conversion of 70% with a 1,3-butadiene yield of 43%.
The Functional Role and Regulatory Mechanism of Bromodomain-Containing Protein 9 in Human Uterine Leiomyosarcoma
Authors: Yang Qiwei , Bariani Maria Victoria , Falahati Ali , Khosh Azad , Lastra Ricardo R. , Siblini Hiba , Boyer Thomas G. , Al-Hendy Ayman
Published: July 2022

Uterine leiomyosarcoma (uLMS) is the most common type of uterine sarcoma associated with poor prognosis, high rates of recurrence, and metastasis. There is currently limited information about uLMS molecular mechanisms of origin and development. Bromodomain (BRD)-containing proteins are involved in many biological processes, most notably epigenetic regulation of transcription, and BRD protein dysfunction has been linked to many diseases including tumorigenesis. However, the role of BRD proteins in the pathogenesis of uLMS is unknown. Here, we show for the first time that BRD9 is aberrantly overexpressed in uLMS tissues compared to adjacent myometrium. BRD9 expression is also upregulated in uLMS cell lines compared to benign uterine fibroid and myometrium cell lines. Inhibition of BRD9 using the specific inhibitor (TP-472) suppressed uLMS cell proliferation via inducing apoptosis and cell cycle arrest. To further characterize the mechanistic basis for TP-472 inhibition of uLMS cell growth, we performed a comparative RNA-seq analysis of vehicle-treated and TP-472-treated uLMS cells (n = 4 each). Bioinformatics analysis revealed that TP-472 treatment distinctly altered the uLMS cell transcriptome. Gene set enrichment analysis identified critical pathways altered by BRD9 inhibition, including interferon-alpha response, KRAS signaling, MYC targets, TNF-a signaling via NFkB, and MTORC1 signaling. Parsimonious gene correlation network analysis identified nine enriched modules, including cell cycle and apoptosis modules. Moreover, the ENCODE Histone Modifications gene set and TargetScan microRNA analysis in Enrichr suggested that TP-472-induced BRD9 inhibition may alter the uLMS cell transcriptome by reprograming the oncogenic epigenome and inducing miRNA-mediated gene regulation. Therefore, BRD9 constitutes a specific vulnerability in malignant uLMS, and targeting non-BET BRD proteins in uLMS may provide a promising and novel strategy for treating patients with this aggressive uterine cancer.
The Effects of Different Pediatric Drugs and Brushing on the Color Stability of Esthetic Restorative Materials Used in Pediatric Dentistry: An In Vitro Study
Authors: Almutairi Manal , Moussa Ihab , Alsaeri Norah , Alqahtani Alhanouf , Alsulaiman Shahad , Alhajri Maram
Published: July 2022

The aim of the current study is to observe how different pediatric drugs and tooth brushing affect the color stability of different esthetic restorative materials. Three restorative materials (composite, compomer, and glass ionomer cement (GIC)) were each used to produce 72 specimens (10 mm × 2 mm). The specimens were divided into six groups and immersed in distilled water and five different pediatric drugs (amoxicillin, ibuprofen, ventolin, paracetamol, and multivitamins). Each group was divided into two subgroups (brushed and non-brushed). Over the course of two weeks, the specimens were agitated for one minute every eight hours. Color changes in all the specimens were evaluated using a spectrophotometer at 1 and 2 weeks. GIC showed a change in color that was significantly greater than that in all the other materials in each solution, except for those in amoxicillin. After a period of 1 to 2 weeks, the most noticeable change in color was detected in the amoxicillin composite and amoxicillin GIC unbrushed groups, and after 2 weeks, a significant difference was found in the ventolin GIC unbrushed group. The color stability of the restorative materials used in pediatric dentistry can be influenced by using popular liquid pediatric medications. GIC was the least color-stable material when subjected to liquid medications.
Evaluation of Half-Cell Potential Measurements for Reinforced Concrete Corrosion
Authors: Almashakbeh Yousef , Saleh Eman , Al-Akhras Nabil M.
Published: July 2022

The evaluation of half-cell potential measurements in reinforced concrete (RC) members can be a key issue for civil engineers. The primary reason for this is that the interpretation of half-cell potential measurements based on the available standards provides information related only to the possibility of corrosion in concrete, but it does not provide a clear perception of the influence of corrosion on the capacity of the RC members. The objective of this study is two-fold: (1) to explore the influence of corrosion level on the flexural capacity of RC members; and (2) to provide engineers with a better understanding of the correlation between half-cell potential measurements and flexural capacity of RC members. To establish this, twelve RC beams were cast and then exposed to accelerated corrosion utilizing an impressed current. After that, half-cell potential tests were performed on the entire surface of the beams. Next, a four-point loading test was performed on the beams to determine their flexural behavior. The analysis of measurements showed that there is a high positive correlation between the half-cell potential measurements and the flexural capacity of the tested beams which demonstrates the potential of half-cell measurements to predict the capacity degradation level of the RC beams due to corrosion.
Concept of High-Tech Enterprise Development Management in the Context of Digital Transformation
Authors: Pronchakov Yurii , Prokhorov Oleksandr , Fedorovich Oleg
Published: July 2022

The purpose of this article is to check and identify management gaps that lead to the formation of digitalization problems in enterprises in the context of Industry 4.0 and to offer a conceptual approach to managing the development of high-tech enterprises in digital transformation. The paper substantiates the concept of digital transformation management in a high-tech enterprise based on interdependent adaptive systems for planning digital transformation processes, monitoring, and change management. The paper considers the idea of the Industry 4.0 concept and presents principal technologies and tools that contribute to the gradual transition to digital transformation. It is determined that digital transformation is a process of transition to digital business, which involves the use of digital technologies to change business processes in the company and provision of new opportunities for additional income and development prospects. A conceptual model of enterprise competitiveness formation in the process of digital transformation has been developed, which includes organizational and economical digital tools for sustainable development of high-tech enterprises and synergies from the organization of new forms of digital interaction. The proposed methodology for managing the development of high-tech enterprises in the context of digital transformation is based on the formation of an ecosystem model of decentralization in a single distributed digital space, based on interconnected adaptive systems of planning, monitoring, and change management, and, on the basis of modeling and forecasting of complex manufacturing and logistics processes of high-tech industries, it allows effective implementation of the innovative order portfolio in the short term and with limited opportunities while coordinating the priorities of the business strategy and strategy of digital transformation of high-tech enterprises.
Medical-Waste Chain: A Medical Waste Collection, Classification and Treatment Management by Blockchain Technology
Authors: Le Hai Trieu , Quoc Khoi Le , Nguyen The Anh , Dang Khoa Tran , Vo Hong Khanh , Luong Huong Hoang , Le Van Hieu , Gia Khiem Huynh , Cao Phu Loc Van , Nguyen Truong Quoc Duy , Huyen Nguyen Tran , Son Ha Xuan , Duong-Trung Nghia
Published: July 2022

To prevent the spread of the COVID-19 pandemic, 2019 has seen unprecedented demand for medical equipment and supplies. However, the problem of waste treatment has not yet been given due attention, i.e., the traditional waste treatment process is done independently, and it is not easy to share the necessary information. Especially during the COVID-19 pandemic, the interaction between parties is minimized to limit infections. To evaluate the current system at medical centers, we also refer to the traditional waste treatment processes of four hospitals in Can Tho and Ho Chi Minh cities (Vietnam). Almost all hospitals are handled independently, lacking any interaction between the stakeholders. In this article, we propose a decentralized blockchain-based system for automating waste treatment processes for medical equipment and supplies after usage among the relevant parties, named Medical-Waste Chain. It consists of four components: medical equipment and supplies, waste centers, recycling plants, and sorting factories. Medical-Waste Chain integrates blockchain-based Hyperledger Fabric technology with decentralized storage of medical equipment and supply information, and securely shares related data with stakeholders. We present the system design, along with the interactions among the stakeholders, to ensure the minimization of medical waste generation. We evaluate the performance of the proposed solution using system-wide timing and latency analysis based on the Hyperledger Caliper engine. Our system is developed based on the hybrid-blockchain system, so it is fully scalable for both on-chain and off-chain-based extensions. Moreover, the participants do not need to pay any fees to use and upgrade the system. To encourage future use of Medical-Waste Chain, we also share a proof-of-concept on our Github repository.