bluuebunny/crossref_metadata_2025 huggingface dataset:
Capturing and Operationalizing Participation in Pediatric Re/Habilitation Research Using Artificial Intelligence: A Scoping Review
Authors: Kaelin Vera C. , Valizadeh Mina , Salgado Zurisadai , Sim Julia G. , Anaby Dana , Boyd Andrew D. , Parde Natalie , Khetani Mary A.
Published: April 2022

Background

There is increased interest in using artificial intelligence (AI) to provide participation-focused pediatric re/habilitation. Existing reviews on the use of AI in participation-focused pediatric re/habilitation focus on interventions and do not screen articles based on their definition of participation. AI-based assessments may help reduce provider burden and can support operationalization of the construct under investigation. To extend knowledge of the landscape on AI use in participation-focused pediatric re/habilitation, a scoping review on AI-based participation-focused assessments is needed.

Objective

To understand how the construct of participation is captured and operationalized in pediatric re/habilitation using AI.

Methods

We conducted a scoping review of literature published in Pubmed, PsycInfo, ERIC, CINAHL, IEEE Xplore, ACM Digital Library, ProQuest Dissertation and Theses, ACL Anthology, AAAI Digital Library, and Google Scholar. Documents were screened by 2–3 independent researchers following a systematic procedure and using the following inclusion criteria: (1) focuses on capturing participation using AI; (2) includes data on children and/or youth with a congenital or acquired disability; and (3) published in English. Data from included studies were extracted [e.g., demographics, type(s) of AI used], summarized, and sorted into categories of participation-related constructs.

Results

Twenty one out of 3,406 documents were included. Included assessment approaches mainly captured participation through annotated observations (n = 20; 95%), were administered in person (n = 17; 81%), and applied machine learning (n = 20; 95%) and computer vision (n = 13; 62%). None integrated the child or youth perspective and only one included the caregiver perspective. All assessment approaches captured behavioral involvement, and none captured emotional or cognitive involvement or attendance. Additionally, 24% (n = 5) of the assessment approaches captured participation-related constructs like activity competencies and 57% (n = 12) captured aspects not included in contemporary frameworks of participation.

Conclusions

Main gaps for future research include lack of: (1) research reporting on common demographic factors and including samples representing the population of children and youth with a congenital or acquired disability; (2) AI-based participation assessment approaches integrating the child or youth perspective; (3) remotely administered AI-based assessment approaches capturing both child or youth attendance and involvement; and (4) AI-based assessment approaches aligning with contemporary definitions of participation.
Active Stabilization of Interventional Tasks Utilizing a Magnetically Manipulated Endoscope
Authors: Barducci Lavinia , Scaglioni Bruno , Martin James , Obstein Keith L. , Valdastri Pietro
Published: April 2022

Magnetically actuated robots have become increasingly popular in medical endoscopy over the past decade. Despite the significant improvements in autonomy and control methods, progress within the field of medical magnetic endoscopes has mainly been in the domain of enhanced navigation. Interventional tasks such as biopsy, polyp removal, and clip placement are a major procedural component of endoscopy. Little advancement has been done in this area due to the problem of adequately controlling and stabilizing magnetically actuated endoscopes for interventional tasks. In the present paper we discuss a novel model-based Linear Parameter Varying (LPV) control approach to provide stability during interventional maneuvers. This method linearizes the non-linear dynamic interaction between the external actuation system and the endoscope in a set of equilibria, associated to different distances between the magnetic source and the endoscope, and computes different controllers for each equilibrium. This approach provides the global stability of the overall system and robustness against external disturbances. The performance of the LPV approach is compared to an intelligent teleoperation control method (based on a Proportional Integral Derivative (PID) controller), on the Magnetic Flexible Endoscope (MFE) platform. Four biopsies in different regions of the colon and at two different system equilibria are performed. Both controllers are asked to stabilize the endoscope in the presence of external disturbances (i.e. the introduction of the biopsy forceps through the working channel of the endoscope). The experiments, performed in a benchtop colon simulator, show a maximum reduction of the mean orientation error of the endoscope of 45.8% with the LPV control compared to the PID controller.
Probiotics for Oral Candidiasis: Critical Appraisal of the Evidence and a Path Forward
Authors: Archambault Linda S. , Dongari-Bagtzoglou Anna
Published: April 2022

Oropharyngeal Candidiasis (OPC) is a mucosal fungal infection that is prevalent among patients with compromised immunity. The success of probiotics in treating chronic diseases with a microbial etiology component at other mucosal sites (i.e., gastro-intestinal, genitourinary and alveolar mucosae) has inspired research into the use of probiotics in the treatment of OPC. A growing body of research in vitro and in animal models indicates that some probiotic species and strains have inhibitory activities against Candida albicans growth, morphological switching, and biofilm formation. However, recent review and meta-analysis studies reveal a dearth of human randomized, controlled clinical trials on the efficacy of probiotics to treat or prevent OPC, while the majority of these have not based their selection of probiotic strains or the type of administration on sound pre-clinical evidence. In this mini-review, we assess the state of the field, outline some of the difficulties in translating lab results to clinical efficacy, and make recommendations for future research needed in order to move the field forward.
How Trees Make Tea
Authors: Stubbins Aron , Ryan Kevin A. , Van Stan John
Published: April 2022

Tea is a mix of natural plant chemicals dissolved in water. You should not drink it, but a weak tea drips through a tree’s branches and runs down its trunk when it is raining. The main ingredients in both tea and tree tea are organic molecules. Some, like tannins, are colorful and give tea and tree tea their brownish colors. Others, including sugars, are clear and loaded with energy. Some molecules build up on the tree’s surface as it sweats. Other molecules are deposited on the tree by the wind, building up like grime on a car. When it rains, the built up sweat and dirt are washed away as tree tea. Tree tea is an energy drink that bacteria on the forest floor crave! Tree tea is also a flow of carbon from trees to soils that researchers are just beginning to understand.
What Is Personality?
Authors: Kramer Robin S. S. , Gosling Samuel D.
Published: April 2022

When you describe yourself and the people you know, the words you use might be different for each person. Maybe your sister is creative, and your brother is friendly. You could even describe your dog as playful and your neighbor’s cat as nervous! The descriptions are different because each of you has a different personality—that is, you differ from others in terms of how you feel, think, and behave. But where does your personality come from? Why are people different? Will your personality always be the same as it is now? Do animals have personalities too? In this article, we discuss what researchers know about personality, how it is studied, and what we all can learn from it.
The Social Science of Institutional Transformation: Intersectional Change in the Academy
Authors: Morimoto Shauna A.
Published: April 2022

This article examines intersectional praxis as an approach to institutional transformation, arguing that intersectionality is both a catalyst for and outcome of gender equity efforts in the social sciences and other academic STEM fields. As such, approaching gender equity intersectionally can be understood as a way that theory and practice are co-constitutive in social science and hence an important aspect of transforming academic institutions. Through a case study of the US National Science Foundation (NSF) ADVANCE program for gender equity in STEM, I look at the development of ADVANCE from an effort to support women in scientific fields to becoming a program for institutional transformation grounded in an intersectional understanding of women's inequity in the academic labor force. I ask two related questions in the efforts to address gender inequities in STEM. First, what is the relationship between academic institutions (which are simultaneously sites for the discovery of knowledge and gender inequality) and the National Science foundation, as the premier American academic institutional funding agency? Second, how has this relationship, through those working on ADVANCE, fundamentally shifted the understanding of the social scientific tools and strategies necessary to advance equity for women in academia? In looking at these questions, I argue that, beyond women's representation in social sciences and academia broadly, intersectionality is an important scholarly advance in social science that offers a dialectical tool for change.
Magnetic Field Re-configuration Associated With a Slow Rise Eruptive X1.2 Flare in NOAA Active Region 11944
Authors: Yurchyshyn Vasyl , Yang Xu , Nita Gelu , Fleishman Gregory , Abramenko Valentina , Inoue Satoshi , Lim Eun-Kyung , Cao Wenda
Published: April 2022

Using multi-wavelength observations, we analysed magnetic field variations associated with a gradual X1.2 flare that erupted on January 7, 2014 in active region (AR) NOAA 11944 located near the disk center. A fast coronal mass ejection (CME) was observed following the flare, which was noticeably deflected in the south-west direction. A chromospheric filament was observed at the eruption site prior to and after the flare. We used SDO/HMI data to perform non-linear force-free field extrapolation of coronal magnetic fields above the AR and to study the evolution of AR magnetic fields prior to the eruption. The extrapolated data allowed us to detect signatures of several magnetic flux ropes present at the eruption site several hours before the event. The eruption site was located under slanted sunspot fields with a varying decay index of 1.0-1.5. That might have caused the erupting fields to slide along this slanted magnetic boundary rather than vertically erupt, thus explaining the slow rise of the flare as well as the observed direction of the resulting CME. We employed sign-singularity tools to quantify the evolutionary changes in the model twist and observed current helicity data, and found rapid and coordinated variations of current systems in both data sets prior to the event as well as their rapid exhaustion after the event onset.
ULF Wave Modeling, Effects, and Applications: Accomplishments, Recent Advances, and Future
Authors: Hartinger Michael D. , Takahashi Kazue , Drozdov Alexander Y. , Shi Xueling , Usanova Maria E. , Kress Brian
Published: April 2022

Ultra Low Frequency (ULF) waves play important roles in magnetosphere-ionosphere coupling, ring current and radiation belt dynamics, and modulation of higher frequency wave modes and energetic particle precipitation. The “ULF wave modeling, effects, and applications” (UMEA) focus group - part of the Geospace Environment Modeling effort from 2016 to 2021 - sought to improve understanding of the physics of ULF waves and their specification in geospace models. Through a series of in person and virtual meetings the UMEA focus group brought modelers and experimentalists together to compare ULF wave outputs in different models, plan observation campaigns focused on ULF waves, discuss recent advances in ULF wave research, and identify unresolved ULF wave science questions. This article summarizes major discussion points and accomplishments in the UMEA focus group over the last 6 years, recent advances and their connection to Richard Thorne and Peter Gary’s significant contributions to ULF wave research, and the future of ULF wave research.
Mental Health in German Paralympic Athletes During the 1st Year of the COVID-19 Pandemic Compared to a General Population Sample
Authors: Busch Aglaja , Kubosch Eva Johanna , Bendau Antonia , Leonhart Rainer , Meidl Verena , Bretthauer Berit , Petzold Moritz Bruno , Dallmann Petra , Wrobel Nina , Plag Jens , Ströhle Andreas , Hirschmüller Anja
Published: April 2022

Introduction

The COVID-19 pandemic has huge influences on daily life and is not only associated with physical but also with major psychological impacts. Mental health problems and disorders are frequently present in elite paralympic athletes. Due to the pandemic situation, new stressors (e.g., loss of routine, financial insecurity) might act upon the athletes. Therefore, the assessment of mental health in athletes during the COVID-19 pandemic is important to identify prevalence of psychological problems and propose countermeasures.

Methods

The mental health of German paralympic athletes was longitudinally monitored (starting in May 2019). The athletes completed the Patient Health Questionnaire 4 (PHQ-4) on a weekly basis and reported a stress level, training hours, and training load. During the pandemic, 8 measurement time points (March 2020 to April 2021) were used to reflect the psychological health course of the athletes. In parallel, a convenience sample of the general population was questioned about their psychological distress, including the PHQ-4. To be included in the analysis, participants of both groups had to complete at least 4 measurement time points. Matching of the para-athletes and the general population sample was prioritized upon completion of the same measurement time points, gender, and age.

Results

Seventy-eight paralympic athletes (40 women, 38 men, age: 29.8 ± 11.4 years) met the inclusion criteria. Seventy-eight matched pairs of the general population (40 women; 38 men; age: 30.5 ± 10.9 years) were identified. The para-athletes had a significantly (p <0.0001; 0.39 < r <0.48) lower PHQ-4 value at each measurement time point compared to the matched control group. No significant age or sex differences were evident regarding the symptom burden. In para-athletes, no significant and a weak positive correlation was found between decreased training load and PHQ-4 values and a stress level, respectively. Reduced physical activity was significantly (p <0.0001) associated with higher PHQ-4 values in the general population sample.

Discussion

Lower PHQ-4 values were reported by the para-athletes compared to the general population sample. However, small sample sizes must be considered while interpreting the data. Nevertheless, adequate support for individuals suffering from severe psychopathological symptoms should be provided for para-athletes as well as for the general population.
European Food Systems in a Regional Perspective: A Comparative Study of the Effect of COVID-19 on Households and City-Region Food Systems
Authors: Millard Jeremy , Sturla Alberto , Smutná Zdeňka , Duží Barbora , Janssen Meike , Vávra Jan
Published: April 2022

The concept of the city-region food system is gaining attention due to the need to improve food availability, quality and environmental benefits, for example through sustainable agri-food strategies. The COVID-19 pandemic has reinforced the importance of coherent and inclusive food governance, especially regarding food resilience, vulnerability and justice. Given that evidence from good practices is relatively sparse, it is important to better understand the role of different types of cities, regions and household characteristics. The paper's aim is to describe, analyze and attempt to explain (sub-national) regional variations of household food behavior before and during the first wave of COVID-19 in 2020 using a city-region food system perspective. Informed by the literature, comprehensive survey data from 12 countries across Europe is used to describe the pre-pandemic landscape of different household food behaviors across comparable regional types. We examine how a specific economic and social shock can disrupt this behavior and the implications for city-region food systems and policies. Conclusions include the huge disruptions imposed on income-weak households and that the small city scale is the most resilient. Proposals are made that can strengthen European city-region food system resilience and sustainability, especially given that future shocks are highly likely.
Rural Consumer Preferences for Inland Fish and Their Substitutes in the Dry-Zone of Sri Lanka and Implications for Aquaculture Development
Authors: Murray Francis Joseph , Little David Colin
Published: April 2022

Poor understanding of market demand is a key factor in the recurrent failure of many production-orientated aquaculture interventions. In the Dry-Zone of Sri Lanka stocking-based fisheries enhancements in small-scale reservoirs have been promoted by the State since the 1980's with no evidence of sustainable adoption. Through an evaluation of positive (actual) and normative (aspirational) consumer preferences for inland fish and its substitutes, we aimed to assess underlying reasons for this recurrent failure and future prospects for the policy and rural food-security. Work commenced with a detailed scoping-analysis to establish socio-economic context and a sample frame for systematic surveys. Consumption recall and preference ranking/scoring techniques were applied in two iterative survey phases across 6 villages of Northwest Province. Totals of 39 and 165 respondents were surveyed across the two phases with selection stratified on age, gender, caste and wealth criteria. Results underscored the importance of inland fish to rural food security. Fresh inland fish was consumed by 60% of all respondents at least once per fortnight and by 82% at least once per month with tilapias accounting for 75–85% of meals. Preference for inland fish compared over similarly priced substitutes is attributable to their freshness at point of sale above any other intrinsic product quality. Larger inland and marine fish were more popular than smaller sizes of the same varieties though poorer households consumed lower cost small fresh tilapia (<180 g) and dried marine fish at higher frequency. Common carp an exotic candidate for culture-based reservoir stocking enhancements, held an intermediate position. National policies on inland aquaculture development and rural food security are discussed and recommendations made in the light of these findings.
Preliminary Results of the First 50 Patients Undergoing Sclerotherapy for II-Degree Hemorrhoidal Disease Using an Automated Device
Authors: Goglia Marta , Nigro Casimiro , Aurello Paolo , Diaco Elia , Trompetto Mario , Gallo Gaetano
Published: April 2022

Background

Sclerotherapy is defined as the injection of sclerosant agents causing fibrosis and scarring of the surrounding tissue. It is currently employed for the treatment of I-III degree hemorrhoidal disease (HD). The aim of this study is to investigate the use of a new automated device for the injection of 3% polidocanol foam.

Methods

This is an observational study including 50 patients who underwent a sclerotherapy procedure with 3% polidocanol foam for II-degree HD according to Goligher classification. Patients were evaluated through validated scores [Giamundo score, Hemorrhoidal Disease Symptom Score (HDSS), Short Health Scale (SHS-HD) and Vaizey score]. Follow-up was conducted until 3 months from the procedure.

Results

Complete resolution of bleeding was achieved in 72% and 78% of patients, respectively, at 1 week and after 3 months from the procedure. Forty eight percent of patients were symptom free after the last follow-up visit (HDSS = 0). No major surgical complications were reported. Three patients out of 36 successfully treated, recurred, and needed a second sclerotherapy injection, which was successful in 2 of them.

Conclusion

These preliminary results of 3% polidocanol foam injection on 50 patients suggest the efficacy and reproducibility of the technique with this new device in the short-term follow-up.
Plumage and Fat Condition Scores as Well-Being Assessment Indicators in a Small Passerine Bird, the Zebra Finch (Taeniopygia guttata)
Authors: Kalnins Lisa , Krüger Oliver , Krause E. Tobias
Published: April 2022

The well-being of animals kept by humans in laboratories, farms, or as pets should always be of the highest importance, and a prerequisite for this is adequate housing. To assess the potential indicators for animal well-being, ideally simple, non-invasive, and reliable methods are necessary. Here, we propose a novel plumage scoring system for small songbirds, using the example of the Zebra Finch, and examine its reliability in comparison with a well-known body condition index, that is, the fat score. We used up to five different observers of different experience levels to assess inter- and intra-observer reliability of the proposed plumage score and also the fat score. We found substantial inter-observer reliability for the proposed novel plumage score, and lower inter-observer reliability for the fat score, which seems to require more training of observers. The intra-observer reliability of the experienced observer who trained the others also showed a very strong reliability for the plumage score and for the fat score. Thus, we conclude that our proposed novel plumage score is a simple, reliable, and non-invasive way to estimate an important indicator of captive Zebra Finches' well-being. Furthermore, the plumage score can be reliably taught to other observers. The plumage score, maybe in combination with the fat score, may be an important tool to reliably assess well-being on a regular basis in captive populations in zoos, laboratories, or pet stocks.
Assessing the Risk of Commercial Vaccines Against Pseudorabies Virus in Cats
Authors: Tu Lu , Zhao Jingjie , Chen Qiuyang , Zhang Shan , Liang Lin , Tang Xinming , Hou Shaohua , Yang Weifang , Liang Ruiying
Published: April 2022

Pseudorabies virus (PRV) is a zoonotic agent that causes significant economic losses in animal husbandry worldwide, and gE-deleted vaccines play an important role in its treatment in the swine industry. However, the potential risk of attenuated PRV strains in commercial vaccines for other hosts remains unclear. Especially, cats are important companion animals for human beings. In this study, we investigated the prevalence and pathogenicity of the PRV wild strain in the cat population. We found that the occurrence of PR diseases in cats is sporadic, that the attenuated PRV strain causes slight clinical signs in cats, and that the virus is excreted 3 days post-infection. Our findings will be beneficial in furthering our understanding of the epidemiology and pathogenicity of PRV in cats and implying the great risk of RPV transmission from pigs to cats.
Evaluation of a Standardized Protocol for Plasma Rich in Growth Factors Obtention in Cats: A Prospective Study
Authors: Miguel-Pastor Laura , Satué Katy , Chicharro Deborah , Torres-Torrillas Marta , del Romero Ayla , Peláez Pau , Carrillo José M. , Cuervo Belén , Sopena Joaquín J. , Cerón José J. , Rubio Mónica
Published: April 2022

Introduction

Platelet-rich plasma (PRP) is an autologous plasma with platelet (PLT) concentration above that of whole blood (WB). PLTs contain growth factors (GFs) that promote tissular repair.

Objectives

To determine and compare the concentrations of PLT, red blood cells (RBC) and white blood cells (WBC) between WB samples, PRP and platelet poor plasma (PPP) samples; and to analyze the concentrations of platelet-derived growth factor BB (PDGF-BB) and transforming growth factor β1 (TGF-β1) in the PRP and PPP of healthy adult cats using a standardized protocol with PRGF®-Endoret® characteristics.

Material and Methods

WB was collected from 30 cats. PRP was obtained following three centrifugation protocols using PRGF®-Endoret® technology: 255, 260, and 265 g for 10 min each. The cellular components, RBC, WBC, PLT, and the concentrations of PDGF-BB and TGF-β1 in the PRP and PPP fractions were determined for each protocol.

Results

PLTs in the PRP fraction were statistically higher than WB, with no statistical differences between PPP and WB. In PRP fraction, PLT concentration was increased 1.4 times on average at 255 g; 1.3 times at 260 g and, 1.5 times at 265 g without statistical differences among them. The mean platelet volume (MPV) was significantly higher in WB compared to PRP and PPP fractions without significant differences between protocols. Compared to WB, the number of RBCs and WBCs was reduced by 99% and by more than 95% in PRP and PPP respectively, without significant differences between protocols. PDGF-BB concentrations were statistically higher in PRP than in PPP fractions, however, TGF-ß1 concentrations did not vary between fractions at 260 g. Comparing the three protocols within PRP and PPP fractions, no differences in PDGF-BB and TGF-ß1 concentrations were observed.

Clinical Relevance

The study shows scientific evidence regarding the obtention of PRP in cats using the PRGF®-Endoret® technology for the quantification of PDGF-BB and TGF-ß1. At 265 g for 10 min, PLT concentration was increased 1.5 times with unnoticeable erythrocytes and leukocytes in the samples. These results clearly show that the PRGF®-Endoret® methodology is suitable to obtain PRP in cats. Further studies are needed to determine the clinical efficacy of the obtained PGRF in the treatment of different pathologies in cats.
Investigation of Beraprost Sodium on Cardiac Function and Hemodynamics in Canine Models of Chronic Pulmonary Hypertension
Authors: Suzuki Ryohei , Yuchi Yunosuke , Saito Takahiro , Teshima Takahiro , Matsumoto Hirotaka , Koyama Hidekazu
Published: April 2022

Pulmonary hypertension (PH) is a life-threatening disease in dogs characterized by increased pulmonary arterial pressure (PAP) and/or pulmonary vascular resistance. No study has evaluated the utility of Beraprost sodium (BPS) in dogs with PH. This study aimed to evaluate the effect of BPS on cardiac function and hemodynamics and examine the optimal dose of BPS in canine models of chronic embolic PH. In this prospective crossover study, three doses of BPS (5, 15, and 25 μg/kg, twice a day) were examined in eight canine models of chronic embolic PH. All model dogs underwent invasive PAP measurement, echocardiography, and non-invasive systemic blood pressure measurement before and after continuous administration of oral BPS for 1 week. No side effects of BPS were observed in any dog during the study. All doses of BPS significantly decreased systolic PAP and pulmonary vascular impedance. Additionally, systemic vascular impedance significantly decreased with 15 and 25 μg/kg of BPS. The right ventricular stroke volume and longitudinal strain significantly decreased with all doses of BPS. The left ventricular stroke volume and circumferential strain decreased with 15 μg/kg BPS. BPS was well-tolerated in this study. A dose-dependent vasodilating effect on pulmonary vessels was observed in canine models of chronic PH. Additionally, 15 μg/kg BPS showed a balanced vasodilating effect on systemic and pulmonary vessels. Furthermore, with a decrease in systemic and pulmonary vascular impedance, the left and right ventricular functions were significantly improved. Our results suggest that BPS may be useful in the treatment of canine PH.
Mortality Burden due to Exposure to Outdoor Fine Particulate Matter in Hanoi, Vietnam: Health Impact Assessment
Authors: Nhung Nguyen T. T. , Jegasothy Edward , Ngan Nguyen T. K. , Truong Ngo X. , Thanh Nguyen T. N. , Marks Guy B. , Morgan Geoffrey G.
Published: April 2022

Objective: This study reports the mortality burden due to PM2.5 exposure among adults (age >25) living in Hanoi in 2017.

Methods: We applied a health impact assessment methodology with the global exposure mortality model and a PM2.5 map with 3 × 3 km resolution derived from multiple data sources.

Results: The annual average PM2.5 concentration for each grid ranged from 22.1 to 37.2 µg/m³. The district average concentration values ranged from 26.9 to 37.2 µg/m³, which means that none of the 30 districts had annual average values below the Vietnam Ambient National Standard of 25 µg/m3. Using the Vietnam Ambient National Standard as the reference standard, we estimated that 2,696 deaths (95% CI: 2,225 to 3,158) per year were attributable to exposure to elevated PM2.5 concentrations in Hanoi. Using the Interim Target 4 value of 10 µg/m3 as the reference standard, the number of excess deaths attributable to elevated PM2.5 exposure was 4,760 (95% CI: 3,958–5,534).

Conclusion: A significant proportion of deaths in Hanoi could be avoided by reducing air pollution concentrations to a level consistent with the Vietnam Ambient National Standard.
The Ideological Component in 21st Century Terrorism
Authors: Tomasiewicz Jarosław
Published: April 2022

The article analyzes transformation of the ideological factor of terrorism in 21st century. The author emphasizes transition from grand narratives (Marxism, fascism) to little narratives of new social movements. In his opinion terrorism without ideology is more dangerous than ideological terrorism.
Grassland Model Based Evaluation of Drought Indices: A Case Study from the Slovenian Alpine Region
Authors: Pogačar Tjaša , Žnidaršič Zala , Vlahović Živa , Črepinšek Zalika , Sušnik Andreja
Published: April 2022

Considering that the relationships between agrometeorological drought indices and the impact of agricultural drought in Slovenia are not yet well understood, the aim of this study was to make an indicative evaluation of the success of selected drought indices, addressing topsoil layer and vegetation condition, in detecting drought in agriculture. In this study, the performance of two established drought indices—the SPEI (standardised precipitation evapotranspiration index) and the VHI (vegetation health index)—was evaluated with respect to yield values calculated with the LINGRA-N model, specifically, the ratio between actual and potential transpiration, also known as drought factor (TRANRF), actual root zone water content (SMACT), leaf area index (LAI), reserve dry weight (WRE), and root dry weight (WRT). The two grassland species selected for analysis were Dactylis glomerata L. (dg) and Lolium perenne L. (lp). For the period 2002–2020 or 2015–2020, three farm sites in Slovenia were considered for evaluation, with two farms at a higher altitude site and one farm at a lower altitude site in the Alpine region. Evaluation of the yield data with the drought indices showed that the r2 values of the linear regression for the selected years with the highest drought impacts (2003, 2013, and 2017) were highest between the two SPEI indices (SPEI-2, SPEI-3) and the model variables TRANRF, SMACT, and WRT, with r2 higher than 0.5 and statistically significant for the lower situated farm in 2013. For 2003 and 2017, the r2 values were less significant as well as for the model variable WRE for all three years selected for analysis (2003, 2013, and 2017).
Effect of Castration Type and Diet on Growth Performance, Serum Sex Hormones and Metabolites, and Carcass Quality of Heavy Male Pigs
Authors: Pérez-Ciria Leticia , Miana-Mena Francisco Javier , Álvarez-Rodríguez Javier , Latorre Maria Angeles
Published: April 2022

A trial was carried out to study the effect of type of castration and diet on pigs destined for Teruel ham production, which is a Spanish protected designation of origin for dry-cured ham. A total of 144 Duroc × (Landrace × Large White) male pigs were used. Half of them were surgically castrated and the other half were immunocastrated with three doses at approximately 25, 58 and 79 kg of body weight. Furthermore, three diets (control vs. high energy vs. low crude protein-CP- and amino acids-AA) were tested from 80 to 137 kg of body weight. Growth performance, serum sex hormones and metabolites, and carcass quality were evaluated. Immunocastrated males grew faster and had better feed conversion ratio than surgically castrated males, but presented lower carcass fatness. Pigs fed the high-energy diet and the low-CP and -AA diet were more efficient at transforming feed into gain than those fed the control diet, but no effect was detected on carcass quality. In conclusion, surgically castrated males are preferable than immunocastrated males for Teruel dry-cured ham elaboration. Besides, a high-energy diet or a low-CP and -AA diet might improve productive performances, but does not provide any benefit in terms of carcass quality.
Bicarbonate-Triggered In Vitro Capacitation of Boar Spermatozoa Conveys an Increased Relative Abundance of the Canonical Transient Receptor Potential Cation (TRPC) Channels 3, 4, 6 and 7 and of CatSper-γ Subunit mRNA Transcripts
Authors: Lacalle Estíbaliz , Consuegra César , Martínez Cristina A. , Hidalgo Manuel , Dorado Jesús , Martínez-Pastor Felipe , Álvarez-Rodríguez Manuel , Rodríguez-Martínez Heriberto
Published: April 2022

Sperm capacitation is a stepwise complex biochemical process towards fertilization. It includes a crucial early calcium (Ca2+) transport mediated by CatSper channels and Canonical Transient Potential Channels (TRPC). We studied the relative abundance of mRNA transcripts changes of the CatSper β, γ and δ subunits and TRPC-channels 1, 3, 4, 6 and 7 in pig spermatozoa, after triggering in vitro capacitation by bicarbonate ions at levels present in vivo at the fertilization site. For this purpose, we analyzedfive5 ejaculate pools (from three fertile adult boars) before (control-fresh samples) and after in vitro exposure to capacitation conditions (37 mM NaHCO3, 2.25 mM CaCl2, 2 mM caffeine, 0.5% bovine serum albumin and 310 mM lactose) at 38 °C, 5% CO2 for 30 min. In vitro capacitation using bicarbonate elicits an increase in the relative abundance of mRNA transcripts of almost all studied Ca2+ channels, except CatSper-δ and TRPC1 (significantly reduced). These findings open new avenues of research to identify the specific role of each channel in boar sperm capacitation and elucidate the physiological meaning of the changes on sperm mRNA cargo.
Apoptotic p53 Gene Expression in the Regulation of Persistent Organic Pollutant (POP)-Induced Oxidative Stress in the Intertidal Crab Macrophthalmusjaponicus
Authors: Park Kiyun , Kwak Ihn-Sil
Published: April 2022

Persistent organic pollutants (POPs), some of the most dangerous chemicals released into the aquatic environment, are distributed worldwide due to their environmental persistence and bioaccumulation. In the study, we investigated p53-related apoptotic responses to POPs such as hexabromocyclododecanes (HBCDs) or 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) in the mud crab Macrophthalmus japonicus. To do so, we characterized M. japonicus p53 and evaluated basal levels of p53 expression in different tissues. M. japonicus p53 has conserved amino acid residues involving sites for protein dimerization and DNA and zinc binding. In phylogenetic analysis, the homology of the deduced p53 amino acid sequence was not high (67–70%) among crabs, although M. japonicus p53 formed a cluster with one clade with p53 homologs from other crabs. Tissue distribution patterns revealed that the highest expression of p53 mRNA transcripts was in the hepatopancreas of M. japonicus crabs. Exposure to POPs induced antioxidant defenses to modulate oxidative stress through the upregulation of catalase expression. Furthermore, p53 expression was generally upregulated in the hepatopancreas and gills of M. japonicus after exposure to most concentrations of HBCD or BDE-47 for all exposure periods. In hepatopancreas tissue, significant increases in p53 transcript levels were observed as long-lasting apoptotic responses involving cellular defenses until day 7 of relative long-term exposure. The findings in this study suggest that exposure to POPs such as HBCD or BDE-47 may trigger the induction of cellular defense processes against oxidative stress, including DNA repair, cell cycle arrest, and apoptosis through the transcriptional upregulation of p53 expression in M. japonicus.
Protective Effects against the Development of Alzheimer’s Disease in an Animal Model through Active Immunization with Methionine-Sulfoxide Rich Protein Antigen
Authors: Smith Adam S. , Gossman Kyle R. , Dykstra Benjamin , Gao Fei Philip , Moskovitz Jackob
Published: April 2022

The brain during Alzheimer’s disease (AD) is under severe oxidative attack by reactive oxygen species that may lead to methionine oxidation. Oxidation of the sole methionine (Met35) of beta-amyloid (Aβ), and possibly methionine residues of other extracellular proteins, may be one of the earliest events contributing to the toxicity of Aβ and other proteins in vivo. In the current study, we immunized transgenic AD (APP/PS1) mice at 4 months of age with a recombinant methionine sulfoxide (MetO)-rich protein from Zea mays (antigen). This treatment induced the production of anti-MetO antibody in blood-plasma that exhibits a significant titer up to at least 10 months of age. Compared to the control mice, the antigen-injected mice exhibited the following significant phenotypes at 10 months of age: better short and long memory capabilities; reduced Aβ levels in both blood-plasma and brain; reduced Aβ burden and MetO accumulations in astrocytes in hippocampal and cortical regions; reduced levels of activated microglia; and elevated antioxidant capabilities (through enhanced nuclear localization of the transcription factor Nrf2) in the same brain regions. These data collected in a preclinical AD model are likely translational, showing that active immunization could give a possibility of delaying or preventing AD onset. This study represents a first step toward the complex way of starting clinical trials in humans and conducting the further confirmations that are needed to go in this direction.
Experimental Investigation of the Near-Surface Flow Dynamics in Downburst-like Impinging Jets Immersed in ABL-like Winds
Authors: Canepa Federico , Burlando Massimiliano , Hangan Horia , Romanic Djordje
Published: April 2022

Downburst winds are strong downdrafts of cold air that embed into the atmospheric boundary layer (ABL) and produce intense horizontal outflow upon impingement on the ground. They are highly transient and three-dimensional extreme wind phenomena with a limited spatiotemporal structure that often makes the anemometric measurements in nature inadequate for reconstructing their complex flow fields. In the framework of the project THUNDERR, an experimental campaign on downburst outflows has been carried out at the WindEEE Dome at Western University, Canada. The present study analyzes the three-dimensional interaction between downburst (DB) outflows produced as large-scale impinging jets and ABL winds. Most experimental, numerical and analytical models in the literature neglect this flow interplay or treat it in an oversimplistic manner through a vector superposition. We found that the generated near-surface outflow is asymmetric, and a high-intensity wind zone develops at the interface between DB and ABL winds. The time variability of the leading edge of the outflow was investigated by synchronizing all wind measurements across the testing chamber. The three-dimensional flow structure was studied using a refined grid of Cobra probes that sampled the flow at high frequencies. The passage of the primary vortex produced a significant decrease in the height of maximum radial wind speed, predominantly in the ABL-streamwise direction. The turbulence intensity was the highest in the region where DB propagates into oppositely directed ABL winds.
Study of the Treatment of Organic Waste Gas Containing Benzene by a Low Temperature Plasma-Biological Degradation Method
Authors: Li Yu , Lv Jialin , Xu Qi , Cai Yalan , Yang Hailong , Li Yingying , Yao Yanyan , Wang Wenjuan , Liu Nan
Published: April 2022

Volatile organic compounds (VOCs) from the pharmaceutical and chemical industries have been a matter of concern for some years in China. Achieving efficient degradation of chlorobenzene (CB) in waste gas is difficult because of its high volatility and molecular stability. A DBD (dielectric barrier discharge) biological method was proposed to treat chlorobenzene, aiming to control high operating costs and prevent secondary pollution. In this investigation, a DBD biological method was introduced to deal with chlorobenzene by optimization of process parameters. The results showed that the degradation efficiency of chlorobenzene was close to 80% at a hydraulic retention time (HRT) of 85 s when the inlet concentration was 700 mg·m−3 for the biological method. The degradation efficiency of chlorobenzene reached 80% under a discharge voltage of 7 kV, an inlet concentration of 700 mg·m−3 and an HRT of 5.5 s. The degradation efficiency of an integrated system can be increased by 15–20% compared with that of a single biological system. Therefore, this method can be used as a new way to address chlorobenzene pollution in the pharmaceutical and chemical industries.
Honeybees as Bioindicators of Heavy Metal Pollution in Urban and Rural Areas in the South of Italy
Authors: Di Fiore Cristina , Nuzzo Angelo , Torino Valentina , De Cristofaro Antonio , Notardonato Ivan , Passarella Sergio , Di Giorgi Sabrina , Avino Pasquale
Published: April 2022

The honeybee (Apis mellifera L.) has been used in several studies for monitoring the environmental health status in terms of pollution, due to its wide-ranging foraging flights. Based on this consideration, this study aimed to analyze heavy metal pollution in Molise Region (Italy), by investigating five sites characterized by different levels of contamination. Furthermore, the authors carried out a sampling activity for a long period, in order to obtain a complete dataset. In this way, detailed information about the status of the environments was able to be obtained. The main purpose of this work was to assess the health status of Molise Region and to confirm the suitability of honeybees as environmental bioindicators of heavy metal pollution, by analyzing their variability over time and space. Furthermore, the study compared the health status associated with contamination in terms of heavy metals with that in two different areas of Italy, using hierarchical cluster analysis and principal component analysis, to evaluate the correlation existing among the three different areas of Italy. Following the findings, the authors suggest the use of honeybees as a bioindicator for heavy metal pollution in air quality studies.
The Selenoprotein Glutathione Peroxidase 4: From Molecular Mechanisms to Novel Therapeutic Opportunities
Authors: Weaver Kamari , Skouta Rachid
Published: April 2022

The selenoprotein glutathione peroxidase 4 (GPX4) is one of the main antioxidant mediators in the human body. Its central function involves the reduction of complex hydroperoxides into their respective alcohols often using reduced Glutathione (GSH) as a reducing agent. GPX4 has become a hotspot therapeutic target in biomedical research following its characterization as a chief regulator of ferroptosis, and its subsequent recognition as a specific pharmacological target for the treatment of an extensive variety of human diseases including cancers and neurodegenerative disorders. Several recent studies have provided insights into how GPX4 is distinguished from the rest of the glutathione peroxidase family, the unique biochemical properties of GPX4, how GPX4 is related to lipid peroxidation and ferroptosis, and how the enzyme may be modulated as a potential therapeutic target. This current report aims to review the literature underlying all these insights and present an up-to-date perspective on the current understanding of GPX4 as a potential therapeutic target.
Recombinant Klotho Protein Ameliorates Myocardial Ischemia/Reperfusion Injury by Attenuating Sterile Inflammation
Authors: Myung Jinwoo , Beom Jin-Ho , Kim Ju-Hee , Woo Ji-Sun , Park Incheol , Chung Sung-Phil , Chung Yong-Eun , You Je-Sung
Published: April 2022

Currently, no effective therapy and potential target have been elucidated for preventing myocardial ischemia and reperfusion injury (I/R). We hypothesized that the administration of recombinant klotho (rKL) protein could attenuate the sterile inflammation in peri-infarct regions by inhibiting the extracellular release of high mobility group box-1 (HMGB1). This hypothesis was examined using a rat coronary artery ligation model. Rats were divided into sham, sham+ rKL, I/R, and I/R+ rKL groups (n = 5/group). Administration of rKL protein reduced infarct volume and attenuated extracellular release of HMGB1 from peri-infarct tissue after myocardial I/R injury. The administration of rKL protein inhibited the expression of pro-inflammatory cytokines in the peri-infarct regions and significantly attenuated apoptosis and production of intracellular reactive oxygen species by myocardial I/R injury. Klotho treatment significantly reduced the increase in the levels of circulating HMGB1 in blood at 4 h after myocardial ischemia. rKL regulated the levels of inflammation-related proteins. This is the first study to suggest that exogenous administration of rKL exerts myocardial protection effects after I/R injury and provides new mechanistic insights into rKL that can provide the theoretical basis for clinical application of new adjunctive modality for critical care of acute myocardial infarction.
Novel Screen-Printed Sensor with Chemically Deposited Boron-Doped Diamond Electrode: Preparation, Characterization, and Application
Authors: Matvieiev Oleksandr , Šelešovská Renáta , Vojs Marian , Marton Marián , Michniak Pavol , Hrdlička Vojtěch , Hatala Michal , Janíková Lenka , Chýlková Jaromíra , Skopalová Jana , Cankař Petr , Navrátil Tomáš
Published: April 2022

New screen-printed sensor with a boron-doped diamond working electrode (SP/BDDE) was fabricated using a large-area linear antenna microwave chemical deposition vapor system (LA-MWCVD) with a novel precursor composition. It combines the advantages of disposable printed sensors, such as tailored design, low cost, and easy mass production, with excellent electrochemical properties of BDDE, including a wide available potential window, low background currents, chemical resistance, and resistance to passivation. The newly prepared SP/BDDEs were characterized by scanning electron microscopy (SEM) and Raman spectroscopy. Their electrochemical properties were investigated by cyclic voltammetry and electrochemical impedance spectroscopy using inner sphere ([Fe(CN)6]4−/3−) and outer sphere ([Ru(NH3)6]2+/3+) redox probes. Moreover, the applicability of these new sensors was verified by analysis of the anti-inflammatory drug lornoxicam in model and pharmaceutical samples. Using optimized differential pulse voltammetry in Britton–Robinson buffer of pH 3, detection limits for lornoxicam were 9 × 10−8 mol L−1. The oxidation mechanism of lornoxicam was investigated using bulk electrolysis and online electrochemical cell with mass spectrometry; nine distinct reaction steps and corresponding products and intermediates were identified.