bluuebunny/crossref_metadata_2025 huggingface dataset:
Effectiveness and Safety of Pyrotinib, and Association of Biomarker With Progression-Free Survival in Patients With HER2-Positive Metastatic Breast Cancer: A Real-World, Multicentre Analysis
Authors: Chen Qitong , Ouyang Dengjie , Anwar Munawar , Xie Ning , Wang Shouman , Fan Peizhi , Qian Liyuan , Chen Gannong , Zhou Enxiang , Guo Lei , Gu Xiaowen , Ding Boni , Yang Xiaohong , Liu Liping , Deng Chao , Xiao Zhi , Li Jing , Wang Yunqi , Zeng Shan , Hu Jinhui , Zhou Wei , Qiu Bo , Wang Zhongming , Weng Jie , Liu Mingwen , Li Yi , Tang Tiegang , Wang Jianguo , Zhang Hui , Dai Bin , Tang Wuping , Wu Tao , Xiao Maoliang , Li Xiantao , Liu Hailong , Li Lai , Yi Wenjun , Ouyang Quchang
Published: May 2020

Background: Pyrotinib, an irreversible pan-ERBB inhibitor, has shown promising antitumour activity, and acceptable tolerability. This research was conducted to evaluate the actual use and effectiveness of pyrotinib in China, therefore, contributed to solve the problem of real-world data scarcity.

Methods: In this retrospective study, 168 patients who received pyrotinib treatment for HER2-positive metastatic breast cancer (MBC) in Hunan Province from June 2018 to August 2019 were included. Progression-free survival (PFS), tumor mutation burden (TMB), and drug-related adverse events (AEs) after pyrotinib administration were analyzed.

Results: The median PFS (mPFS) time in the 168 participants was 8.07 months. The mPFS times in patients with pyrotinib in second-line therapy (n = 65) and third-or-higher-line therapy (n = 94) were 8.10 months and 7.60 months, respectively. Patients with brain metastases achieved 8.80 months mPFS time. In patients with pyrotinib in third-or-higher-line therapy, patients who had previously used lapatinib still got efficacy but showed a shorter mPFS time (6.43 months) than patients who had not (8.37 months). TMB was measured in 28 patients, K-M curve (P = 0.0024) and Multivariate Cox analysis (P = 0.0176) showed a significant negative association between TMB and PFS. Diarrhea occurred in 98.2% of participants (in any grade) and 19.6% in grade 3–4 AEs.

Conclusion: Pyrotinib is highly beneficial to second-or-higher-line patients or HER2-positive MBC patients with brain metastases. Pyrotinib seems to be a feasible strategy both in combination of chemotherapeutic drugs or as a replacement of lapatinib if diseases progressed. TMB could be a potential predictor for evaluating pyrotinib's effectiveness in HER2-positive MBC.
Phytotoxic Effect of Herbicides on Various Camelina [Camelina sativa (L.) Crantz] Genotypes and Plant Chlorophyll Fluorescence
Authors: Sobiech Łukasz , Grzanka Monika , Kurasiak-Popowska Danuta , Radzikowska Dominika
Published: May 2020

Camelina is an oil plant classified as a minor crop. The small acreage is the main cause of the small amount of plant protection products that are registered for use on camelina plantations. This contributes to difficulties in the protection of this plant. In the conducted experiment, the genetic similarity of genotypes of camelina was compared. The effect of selected herbicides (propaquizafop at rate 70 g a.i. ha−1, quizalofop-p-ethyl at rate 50 g a.i. ha−1, clopyralid at rate 90 g a.i. ha−1, and picloram at rate 24 g a.i. ha−1 applied in the three-four-leaves growth stage of camelina) on six individual genotypes of the plant and plant chlorophyll fluorescence after the use of these substances was also determined. The Przybrodzka variety showed the lowest level of damage in the assessment carried out 42 days after herbicide application and the damages of plants after quizalofop-p-ethyl and propaquizafop was completely gone. The variety Przybrodzka had the lowest genetic similarity to all analyzed genotypes. In other cases, genetic similarity of analyzed genotypes could not be linked to herbicide-related damage. Picloram contributed to the greatest damage to test plants and had the greatest impact on the operation of photosystem II (PSII). However, the level of plant chlorophyll fluorescence parameter values indicates small PSII damage for all substances and the possibility of subsequent plant regeneration. The results of the presented research indicate that it is worth referring to several plant varieties in phytotoxicity studies of herbicides towards arable crops.
Effect of Borrelia burgdorferi Outer Membrane Vesicles on Host Oxidative Stress Response
Authors: Wawrzeniak Keith , Gaur Gauri , Sapi Eva , Senejani Alireza G.
Published: May 2020

Outer membrane vesicles (OMVs) are spherical bodies containing proteins and nucleic acids that are released by Gram-negative bacteria, including Borrelia burgdorferi, the causative agent of Lyme disease. The functional relationship between B. burgdorferi OMVs and host neuron homeostasis is not well understood. The objective of this study was to examine how B. burgdorferi OMVs impact the host cell environment. First, an in vitro model was established by co-culturing human BE2C neuroblastoma cells with B. burgdorferi B31. B. burgdorferi was able to invade BE2C cells within 24 h. Despite internalization, BE2C cell viability and levels of apoptosis remained unchanged, but resulted in dramatically increased production of MCP-1 and MCP-2 cytokines. Elevated secretion of MCP-1 has previously been associated with changes in oxidative stress. BE2C cell mitochondrial superoxides were reduced as early as 30 min after exposure to B. burgdorferi and OMVs. To rule out whether BE2C cell antioxidant response is the cause of decline in superoxides, superoxide dismutase 2 (SOD2) gene expression was assessed. SOD2 expression was reduced upon exposure to B. burgdorferi, suggesting that B. burgdorferi might be responsible for superoxide reduction. These results suggest that B. burgdorferi modulates cell antioxidant defense and immune system reaction in response to the bacterial infection. In summary, these results show that B. burgdorferi OMVs serve to directly counter superoxide production in BE2C neurons, thereby ‘priming’ the host environment to support B. burgdorferi colonization.
Isoniazid Conjugated Magnetic Nanoparticles Loaded with Amphotericin B as a Potent Antiamoebic Agent against Acanthamoeba castellanii
Authors: Iqbal Kawish , Abdalla Sumayah Abdelnasir Osman , Anwar Ayaz , Iqbal Kanwal Muhammad , Shah Muhammad Raza , Anwar Areeba , Siddiqui Ruqaiyyah , Khan Naveed Ahmed
Published: May 2020

The pathogenic free-living amoeba, Acanthamoeba castellanii, is responsible for a rare but deadly central nervous system infection, granulomatous amoebic encephalitis and a blinding eye disease called Acanthamoeba keratitis. Currently, a combination of biguanides, amidine, azoles and antibiotics are used to manage these infections; however, the host cell cytotoxicity of these drugs remains a challenge. Furthermore, Acanthamoeba species are capable of transforming to the cyst form to resist chemotherapy. Herein, we have developed a nano drug delivery system based on iron oxide nanoparticles conjugated with isoniazid, which were further loaded with amphotericin B (ISO-NPs-AMP) to cause potent antiamoebic effects against Acanthamoeba castellanii. The IC50 of isoniazid conjugated with magnetic nanoparticles and loaded with amphotericin B was found to be 45 μg/mL against Acanthamoeba castellanii trophozoites and 50 μg/mL against cysts. The results obtained in this study have promising implications in drug discovery as these nanomaterials exhibited high trophicidal and cysticidal effects, as well as limited cytotoxicity against rat and human cells.
Total Oxidant and Antioxidant Capacity of Gingival Crevicular Fluid and Saliva in Patients with Periodontitis: Review and Clinical Study
Authors: Toczewska Joanna , Maciejczyk Mateusz , Konopka Tomasz , Zalewska Anna
Published: May 2020

Periodontitis is inextricably linked to oxidative-reductive (redox) imbalance. However, little is still known about the resultant ability to scavenge oxygen free radicals in saliva and gingival crevicular fluid in patients with periodontitis. The multitude of enzymatic and non-enzymatic antioxidants and their synergistic effects cause an interest in the evaluation of the total antioxidative capacity. Thus, our study aimed to evaluate the total oxidative and antioxidative activity of gingival crevicular fluid and saliva in the periodontitis, as well as to relate these biomarkers to clinical indices of periodontopathy. Additionally, by calculating the oxidative stress index (OSI), the intensity of redox disturbances was also evaluated. Fifty-eight periodontitis patients were included in the study and divided into two subgroups depending on the severity of the disease. In the non-stimulated/stimulated saliva as well as a gingival crevicular fluid of the study group, we found significantly higher OSI and total oxidant status (TOS) as well as lower total antioxidant capacity (TAC). However, the ability to reduce iron ions (FRAP) was significantly lower only in stimulated and non-stimulated saliva of patients with periodontitis. The examined parameters correlated with the periodontium’s clinical condition, which indicates the exacerbation of the inflammatory process. However, TAC, TOS, OSI, and FRAP did not differentiate individual stages of periodontitis.
State of the Art of Anthocyanins: Antioxidant Activity, Sources, Bioavailability, and Therapeutic Effect in Human Health
Authors: Tena Noelia , Martín Julia , Asuero Agustín G.
Published: May 2020

The antioxidant activity of anthocyanins in food is well known. Numerous antioxidant assays have been proposed to measure the capacity of anthocyanins to prevent the oxidation process that naturally occurs. Different solvents, temperatures, and pH levels are applied in each assay, and these factors should be taken into account in order to obtain useful and reproducible results. The concentration and the structure of these compounds are directly related to their antioxidant capacity and their environment. However, the effectiveness of the anthocyanin ingestion against diseases is also influenced by its bioavailability. Novel methodologies that simulate the digestion process have been developed in order to facilitate the current knowledge of anthocyanins bioavailability. Studies highlight the potential synergy effect between parent compounds and their derivatives (metabolites, conjugated products, and microbe-generated metabolites). The aim of this review is to provide an overview of advantages and disadvantages of the most common methods to determine the antioxidant activity of anthocyanins, chemical structure, and concentration of these compounds in different edible fruits, vegetables, and plants; their bioavailability after intake; as well as the main therapeutic effect described in the scientific literature.
Biological Activity of Phenolic Compounds in Extra Virgin Olive Oils through Their Phenolic Profile and Their Combination with Anticancer Drugs Observed in Human Cervical Carcinoma and Colon Adenocarcinoma Cells
Authors: Torić Jelena , Brozovic Anamaria , Baus Lončar Mirela , Jakobušić Brala Cvijeta , Karković Marković Ana , Benčić Đani , Barbarić Monika
Published: May 2020

The roles of phenolics from olive oils as effective anticancer agents have been documented in various in vitro studies of different cancer cells lines, but the relationship between the phenolic profile of olive oil and its biological activity needs more elucidation. In this study, we analysed phenolic profiles of extra virgin olive oils (EVOOs) from different autochthonous cultivars from Croatia (Oblica, Bjelica, Buža, Žižolera) and investigated the biological effect of EVOO phenolic extracts (EVOO-PEs) on human cervical (HeLa) and human colon (SW48) cancer cell lines alone and in combination with cisplatin (cDDP), carboplatin (CBP), 5-fluorouracil (5-FU) and irinotecan. The quantitative evaluation of olive oil polyphenols was performed by HPLC-DAD and spectrophotometric analysis. The biological effect of EVOO-PEs alone and in combination with anticancer drugs was measured by MTT assay. Analysed EVOO-PEs differ in phenolic profile and inhibited HeLa and SW48 cells in a dose-dependent manner. Further, it is shown that EVOO-PEs (Oblica-Sea, Buža and Žižolera), in combination with anticancer drugs, increase the metabolic activity of HeLa and SW48 cells and have a protective role. These data imply careful consummation of olive oil during chemotherapy of cancer patients.
Role of Cytochrome P450 Enzymes in Plant Stress Response
Authors: Pandian Balaji Aravindhan , Sathishraj Rajendran , Djanaguiraman Maduraimuthu , Prasad P.V. Vara , Jugulam Mithila
Published: May 2020

Cytochrome P450s (CYPs) are the largest enzyme family involved in NADPH- and/or O2-dependent hydroxylation reactions across all the domains of life. In plants and animals, CYPs play a central role in the detoxification of xenobiotics. In addition to this function, CYPs act as versatile catalysts and play a crucial role in the biosynthesis of secondary metabolites, antioxidants, and phytohormones in higher plants. The molecular and biochemical processes catalyzed by CYPs have been well characterized, however, the relationship between the biochemical process catalyzed by CYPs and its effect on several plant functions was not well established. The advent of next-generation sequencing opened new avenues to unravel the involvement of CYPs in several plant functions such as plant stress response. The expression of several CYP genes are regulated in response to environmental stresses, and they also play a prominent role in the crosstalk between abiotic and biotic stress responses. CYPs have an enormous potential to be used as a candidate for engineering crop species resilient to biotic and abiotic stresses. The objective of this review is to summarize the latest research on the role of CYPs in plant stress response.
The Influence of Dissolved Organic Carbon on the Microbial Community Associated with Tetraselmis striata for Bio-Diesel Production
Authors: Kim Sae-Hee , Kim Jin Ho , Baek Seung Ho , Kim Joo-Hwan , Ajani Penelope A. , Park Bum Soo , Han Myung-Soo
Published: May 2020

The green alga Tetraselmis striata is regarded as a suitable candidate microalga for bio-diesel production. Recently, T. striata was cultured near Yeonghueung Island, Korea, in a “marine culturing field”; however, its environmental impacts are not yet studied. We estimated the amount of dissolved organic carbon (DOC) released from T. striata cultivation in the marine culturing field, and we investigated the changes in bacterial composition. Then, we designed and installed a mesocosm for further understanding. From the mesocosm results, the DOC released from the cultivation of T. striata led to changes in bacterial communities, disturbance of the microbial food web structure, rapid depletion of nutrients, and a decrease in dissolved oxygen (DO) and pH. Our novel work demonstrates that large amounts of DOC secreted by large-scale microalgal cultures such as that of T. striata can potentially have a significant impact on the structure and function of the surrounding microbial ecosystem.
Empirical Modeling of Liquefied Nitrogen Cooling Impact during Machining Inconel 718
Authors: Hribersek Matija , Berus Lucijano , Pusavec Franci , Klancnik Simon
Published: May 2020

This paper explains liquefied nitrogen’s cooling ability on a nickel super alloy called Inconel 718. A set of experiments was performed where the Inconel 718 plate was cooled by a moving liquefied nitrogen nozzle with changing the input parameters. Based on the experimental data, the empirical model was designed by an adaptive neuro-fuzzy inference system (ANFIS) and optimized with the particle swarm optimization algorithm (PSO), with the aim to predict the cooling rate (temperature) of the used media. The research has shown that the velocity of the nozzle has a significant impact on its cooling ability, among other factors such as depth and distance. Conducted experimental results were used as a learning set for the ANFIS model’s construction and validated via k-fold cross-validation. Optimization of the ANFIS’s external input parameters was also performed with the particle swarm optimization algorithm. The best results achieved by the optimized ANFIS structure had test root mean squared error ( t e s t   R M S E ) = 0.2620 , and t e s t   R 2 = 0.8585 , proving the high modeling ability of the proposed method. The completed research contributes to knowledge of the field of defining liquefied nitrogen’s cooling ability, which has an impact on the surface characteristics of the machined parts.
Integration of Wavelet Denoising and HHT Applied to the Analysis of Bridge Dynamic Characteristics
Authors: Wang Xinpeng , Huang Shengxiang , Kang Chao , Li Guanqing , Li Chenfeng
Published: May 2020

When the dynamic characteristics of a bridge structure are analyzed though Hilbert–Huang transform (HHT), the noise contained in the bridge dynamic monitoring data may seriously affect the performance of the first natural frequency identification. A time-frequency analysis method that integrates wavelet threshold denoising and HHT is proposed to overcome this deficiency. The denoising effect of the experimental analysis on the simulated noisy signals proves the effectiveness of the proposed method. This method is used to perform denoising pre-processing on the dynamic monitoring data of Sutong Bridge, and the denoised results of different methods are compared and analyzed. Then, the best denoising data are selected as the input data of Hilbert spectrum analysis to identify the structural first natural frequency of the bridge. The results indicate that the wavelet-empirical mode decomposition (EMD) method effectively reduces the interference of random noise and eliminates useless intrinsic modal function (IMF) components, and the excellent properties of the signal evaluation index after denoising make the method suitable for processing non-stationary signals with noise. When Hilbert spectrum analysis is applied to the denoised data, the first natural frequency of the bridge structure can be identified clearly and is consistent with the theoretical calculation. The proposed method can effectively determine the natural vibration characteristics of the bridge structure.
A Compact Laser Pumped 4He Magnetometer with Laser-Frequency Stabilization by Inhomogeneous Light Shifts
Authors: Wang Haidong , Wu Teng , Wang He , Li Sheng , Lin Zaisheng , Peng Xiang , Guo Hong
Published: May 2020

We propose a compact 4He magnetometer realizing magnetic field measurement and laser-frequency stabilization simultaneously in a single 4He atomic cell. The frequency stabilization scheme is based on the asymmetric line shape of magnetic resonance which is induced by spatially inhomogeneous light shifts. We investigate the asymmetric line shape of the magnetic resonance signal theoretically and experimentally in laser pumped 4He magnetometer with the magneto-optical double-resonance configuration. Notice that, due to the asymmetric line shape, the in-phase component of the magnetic resonance signal is shown to have a linear dependence with respect to the laser frequency detuning and is used to actively lock the laser frequency to the resonant point. The method reduces the complexity of the system and improves the stability of the magnetometer, making the laser-pumped 4He magnetometer more compact and portable.
Effect of Missing Vines on Total Leaf Area Determined by NDVI Calculated from Sentinel Satellite Data: Progressive Vine Removal Experiments
Authors: Vélez Sergio , Barajas Enrique , Rubio José Antonio , Vacas Rubén , Poblete-Echeverría Carlos
Published: May 2020

Remote Sensing (RS) allows the estimation of some important vineyard parameters. There are several platforms for obtaining RS information. In this context, Sentinel satellites are a valuable tool for RS since they provide free and regular images of the earth’s surface. However, several problems regarding the low-resolution of the imagery arise when using this technology, such as handling mixed pixels that include vegetation, soil and shadows. Under this condition, the Normalized Difference Vegetation Index (NDVI) value in a particular pixel is an indicator of the amount of vegetation (canopy area) rather than the NDVI from the canopy (as a vigour expression), but its reliability varies depending on several factors, such as the presence of mixed pixels or the effect of missing vines (a vineyard, once established, generally loses grapevines each year due to diseases, abiotic stress, etc.). In this study, a vine removal simulation (greenhouse experiment) and an actual vine removal (field experiment) were carried out. In the field experiment, the position of the Sentinel-2 pixels was marked using high-precision GPS. Controlled removal of vines from a block of cv. Cabernet Sauvignon was done in four steps. The removal of the vines was done during the summer of 2019, matching with the start of the maximum vegetative growth. The Total Leaf Area (TLA) of each pixel was calculated using destructive field measurements. The operations were planned to have two satellite images available between each removal step. As a result, a strong linear relationship (R2 = 0.986 and R2 = 0.72) was obtained between the TLA and NDVI reductions, which quantitatively indicates the effect of the missing vines on the NDVI values.
An Efficient Ultra-Tight GPS/RISS Integrated System for Challenging Navigation Environments
Authors: Karaim Malek , Tamazin Mohamed , Noureldin Aboelmagd
Published: May 2020

The Global Positioning System (GPS) provides an accurate navigation solution in the open sky. However, in some environments such as urban areas or in the presence of signal jamming, GPS signals cannot be easily tracked since they could be harshly attenuated or entirely blocked. This often requires the GPS receiver to go into a signal re-acquisition phase for the corresponding satellite. To avoid the intensive computations necessary for the signal re-lock in a GPS receiver, a robust signal-tracking mechanism that can hold and/or rapidly re-lock on the signals and keep track of their dynamics becomes a necessity. This paper augments a vector-based GPS signal tracking system with a Reduced Inertial Sensor System (RISS) to produce a new ultra-tight GPS/INS integrated system that enhances receivers’ tracking robustness and sensitivity in challenging navigation environments. The introduced system is simple, efficient, reliable, yet inexpensive. To challenge the proposed method with real jamming conditions, real experiment work was conducted inside the Anechoic Chamber room at the Royal Military College of Canada (RMC). The Spirent GSS6700 signal simulator was used to generate GPS signals, and an INS Simulator is used for simulating the inertial measurement unit (IMU) to generate the corresponding trajectory raw data. The NEAT jammer, by NovAtel, was used to generate real jamming signals. Results show a good performance of the proposed method under real signal jamming conditions.
Snap-Through Buckling Mechanism for Frequency-up Conversion in Piezoelectric Energy Harvesting
Authors: Speciale Alessandro , Ardito Raffaele , Baù Marco , Ferrari Marco , Ferrari Vittorio , Frangi Attilio A.
Published: May 2020

This paper describes a piezoelectric energy harvester employing a snap-through buckling (STB) mechanism for frequency-up conversion (FuC). The harvester consists of two main components: a bistable mechanical structure and one piezoelectric cantilever beam. The device is designed by means of analytical methods and numerical simulations. A proof-of-concept prototype is manufactured and tested under low frequency mechanical excitation. Experimental results show that, if the STB is induced, from the second stable configuration back to the undeformed one, the FuC is obtained and the response of the beam presents frequency components in a wide range, even though the resonant frequency of the cantilever beam is not excited. The results are hence in agreement with the expected behavior: if the device, forced in the second stable configuration, is subject to a low-frequency excitation whose amplitude exceeds a threshold, STB is triggered and the ensuing FuC provokes a widening of the beam vibrations frequency range and consequently a significant effectiveness in terms of power output. A maximum power of 4 mW is obtained by using an optimal resistive load as STB from a stable configuration of the bistable mechanism to the other one is triggered; a maximum energy of 4.5 μ J is obtained in case of a rectifier circuit with storage capacitor.
Surface Defect Detection for Mobile Phone Back Glass Based on Symmetric Convolutional Neural Network Deep Learning
Authors: Jiang Jiabin , Cao Pin , Lu Zichen , Lou Weimin , Yang Yongying
Published: May 2020

Defect detection based on machine vision and machine learning techniques has drawn much attention in recent years. Deep learning is very suitable for such segmentation and detection tasks and has become a promising research area. Surface quality inspection is essentially important in the manufacturing of mobile phone back glass (MPBG). Different types of defects are produced because of the imperfection of the manufacturing technique. Unlike general transparent glass, screen printing glass has totally different reflection and scattering characteristics, which means the traditional dark-field imaging system is not suitable for this task. Meanwhile, the imaging system requires high resolution since the minimum defect size can be 0.005 mm2. According to the imaging characteristics of screen printing glass, this paper proposes a coaxial bright-field (CBF) imaging system and low-angle bright-field (LABF) imaging system, and 8K line-scan complementary metal oxide semiconductor(CMOS) cameras are utilized to capture images with the resolution size of 16,000*8092. The CBF system is applied for the weak-scratch and discoloration defects while the LABF system is applied for the dent defect. A symmetric convolutional neural network composed of encoder and decoder structures is proposed based on U-net, which produces a semantic segmentation with the same size as the original input image. More than 10,000 original images were captured, and more than 30,000 defective and non-defective images were manually annotated in the glass surface defect dataset (GSDD). Verified by the experiments, the results showed that the average precision reaches more than 91% and the average recall rate reaches more than 95%. The method is very suitable for the surface defect inspection of screen printing mobile phone back glass.
Safety of Nuclear Power Plants with Respect to the Fault Displacement Hazard
Authors: Katona Tamás János
Published: May 2020

The hazard of permanent ground displacements/deformations can challenge the safety of the nuclear power plants. Increasing knowledge of the hazard and development of methods for structure–fault–displacement interaction motivates the designing of nuclear power plants for permanent ground displacement instead of abandoning the sites that could be affected by this kind of hazard. For the design basis, permanent ground displacement should be defined at the hazard level that complies with the probabilistic criteria for accounting for the natural hazards in the design that also ensure compliance with probabilistic safety criteria. In this paper, a procedure is proposed for the definition of the design basis permanent ground displacement that is based on the deaggregation of seismic design basis hazard. The definition of the displacement for the margin evaluation is also proposed. The feasibility of safe design is also demonstrated for the proposed definition of design basis hazard via qualitative judgement on the sensitivity of the structures, systems and components ensuring the fundamental safety functions with respect to the permanent ground displacement that is supported by existing case studies.
A One-Pot Ultrasound-Assisted Almond Skin Separation/Polyphenols Extraction and its Effects on Structure, Polyphenols, Lipids, and Proteins Quality
Authors: Tabib Malak , Tao Yang , Ginies Christian , Bornard Isabelle , Rakotomanomana Njara , Remmal Adnane , Chemat Farid
Published: May 2020

Almond skin is an important by-product in the almond processing industry, rich in potentially health-promoting phenolic compounds. The objective of this present study is to separate the skin from the almond and extract its polyphenol contents using Ultrasound-Assisted Extraction (UAE) at room temperature. Optimization was performed according to a two-variable central composite design (CCD), and the optimum combination of ultrasonic intensity and extraction temperature was obtained through multi-response optimization: ultrasonic intensity (UI), 9.47 W.cm−2; and temperature, 20 °C for an extraction time of 20 min. Under the above-mentioned conditions, total phenolic content was 258% higher with UAE than silent experiment. Mathematic modelling and microscopic investigations were achieved to enable understanding physical and structural effects of ultrasound on almond skins and comprehension of the mechanism behind the enhancement of mass transfer phenomena. Scanning Electron Microscopy (SEM) showed different acoustic cavitation impacts including fragmentation, sonoporation, and erosion. Extracts were analyzed by ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS), identifying a combination of flavanols, flavanones and non-flavonoids. UAE shows no negative effect on almond proteins and lipids when compared to natural almonds (NS).
Secrecy Energy Efficiency Maximization in an Underlying Cognitive Radio–NOMA System with a Cooperative Relay and an Energy-Harvesting User
Authors: Garcia Carla E. , Camana Mario R. , Koo Insoo
Published: May 2020

Security is considered a critical issue in the deployment of 5G networks because of the vulnerability of information that can be intercepted by eavesdroppers in wireless transmission environments. Thus, physical layer security has emerged as an alternative for the secure enabling of 5G technologies and for tackling this security issue. In this paper, we study the secrecy energy efficiency (SEE) in a downlink underlying cognitive radio (CR)—non-orthogonal multiple access (NOMA) system with a cooperative relay. The system has an energy-harvesting (EH) user and an eavesdropper, where the transmitter provides direct communication with a close secondary user and a distant secondary user via the relay. Our objective is to maximize the SEE of the CR-NOMA system under the constraints of a minimum information rate for the secondary users, a minimum amount of energy harvested by the EH user, and maximum power availability at the transmitter and the relay that still prevents them from causing unacceptable interference with the primary user. The proposed solution to maximize the SEE is based on the low-computational—complexity particle swarm optimization (PSO) algorithm. For validation purposes, we compare the optimization outcomes obtained by the PSO algorithm with the optimal exhaustive search method. Furthermore, we compare the performance of our proposed CR-NOMA scheme with the conventional orthogonal multiple access (OMA) scheme.
An Augmented Reality Tool for Teaching Application in the Agronomy Domain
Authors: Parras-Burgos Dolores , Fernández-Pacheco Daniel G. , Polhmann Barbosa Thomas , Soler-Méndez Manuel , Molina-Martínez José Miguel
Published: May 2020

Nowadays, the combination of new technologies and the use of mobile devices opens up a new range of teaching–learning strategies in different agricultural engineering degrees. This article presents an augmented reality tool that allows for improved spatial viewing for students who have certain difficulties with viewing graphic representations of agronomic systems and devices. This tool is known as ARTID (Augmented Reality for Teaching, Innovation and Design) and consists in a free-access mobile application for devices using the Android operating system. The proposed method provides each exploded drawing or overall drawing with a QR code that can be used by students to view their 3D models by augmented reality in their own mobile devices. An evaluation experience was carried out to assess the validity of the tool on different devices and the acceptance and satisfaction level of this kind of resources in subjects of graphic expression in engineering. Finally, an example of application in the agronomic domain is provided by the 3D virtual model of portable ferticontrol equipment that comprises the different structures and tanks, which, if viewed by conventional graphical representations, may entail a certain level of difficulty. Thanks to this tool, reality can be merged with the virtual world to help favour the understanding of certain concepts and to increase student motivation in agronomy studies.
Fuzzy Supplier Selection Method Based on Smaller-The-Better Quality Characteristic
Authors: Yu Chun-Min , Chen Kuen-Suan , Lai Kuei-Kuei , Hsu Chang-Hsien
Published: May 2020

Many important parts of tool machines all have the important smaller-the-better (STB) quality characteristics. The important STB quality characteristics will impact on the quality of the end-product. At the same time, supplier quality influences the quality and functionality of the end-product, so suppliers must be selected with caution. The six sigma quality index for the STB quality characteristics can directly reflect process quality levels. Besides, this index possesses a mathematical relationship with process yield. Nevertheless, the point estimation will cause the risk of misjudgment, due to sampling errors. As a result, this study applies the confidence interval of the index to a two-tailed fuzzy testing method, in order to select appropriate suppliers. Now that this method is on the basis of the confidence interval, the possibility of misjudgment caused by sampling errors will be reduced, while the precision of the selection will be enhanced. The method can help companies increase product quality, as well as the competitiveness of the industry chain as a whole. Finally, a numerical example is presented to show how to approach this method and its efficacy.
Spectral Reflectance Characteristics and Chlorophyll Content Estimation Model of Quercus aquifolioides Leaves at Different Altitudes in Sejila Mountain
Authors: Zhu Jiyou , He Weijun , Yao Jiangming , Yu Qiang , Xu Chengyang , Huang Huaguo , Mhae B. Jandug Catherine
Published: May 2020

Quercus aquifolioides is one of the most representative broad-leaved plants in Qinghai-Tibet Plateau with important ecological status. So far, understanding how to quickly estimate the chlorophyll content of plants in plateau areas is still an urgent problem. Field Spec 3 spectrometer was used to measure hyperspectral reflectance data of Quercus aquifolioides leaves at different altitudes, and CCI (chlorophyll relative content) of corresponding leaves was measured by a chlorophyll meter. The correlation and univariate linear fitting analysis techniques were used to establish their relationship models. The results showed that: (1) Chlorophyll relative content of Quercus aquifolioides, under different altitude gradients, were significantly different. From 2905 m to 3500 m, chlorophyll relative content increased first and then decreased. Altitude 3300 m was the most suitable growth area. (2) In 350~550 nm, the spectral reflectance was 3500 m > 3300 m > 2905 m. In 750~1100 nm, the spectral reflectivity was 2905 m > 3500 m > 3300 m. (3) There were 4 main reflection peaks and 5 main absorption valleys in the leaf surface spectral reflection curve. While, 750~1400 nm was the sensitive range of leaf spectral response of Quercus aquifolioides. (4) The red edge position and red valley position moved to short wave direction with the increase of altitude, while the yellow edge position and green peak position moved to long wave direction first and then to short wave direction. (5) The correlation curve between the original spectrum and the CCI value was the best between the wavelengths 509~650 nm. The correlation between the first derivative spectrum and CCI value was the best and most stable at 450~500 nm. The green peak reflectance was most sensitive to the relative chlorophyll content of Quercus aquifolioides. The estimation model R2 of green peak reflectance was the highest (y = 206.98e−10.85x, R2 = 0.8523), and the prediction accuracy was 95.85%. The research results can provide some technical and theoretical support for the protection of natural Quercus aquifolioides forests in Tibet.
An Integrated Cooling Jet and Air Curtain System for Stadiums in Hot Climates
Authors: Zhong Fangliang , Calautit John
Published: May 2020

The 2022 FIFA World Cup brings Qatar great challenges in terms of minimizing the cooling energy consumption and providing thermal comfort for both spectators and players. This paper presents comparisons among the results of thermal and wind environment modelling of a semi-outdoor stadium under three different cooling configurations and a baseline configuration without cooling using the Computational Fluid Dynamics (CFD) tool ANSYS Fluent 18.2. The three cooling configurations are: (1) vertical jets only above upper tiers, (2) vertical jets above upper tiers and horizontal jets at the back of lower tiers and around the pitch, (3) integrated vertical jets above upper tiers, horizontal jets at the back of lower tiers and air curtains at gates. De-coupled solar radiation simulations are implemented using the solar irradiance data in Doha under fair weather conditions method in Fluent in order to capture realistic thermal boundary conditions for the ground, stadium and surrounding buildings. On the basis of the set conditions, the results show that air curtains, employed in configuration 3 are effective in preventing the penetration of hot outside air through the gates of the stadium, which is an existing issue for stadiums in hot climates, and also contribute to lower energy consumption per match than the other configurations of cooling jets. The results presented in this study are useful not only for future design and retrofits of stadiums in hot climates but also for stadiums that incorporate mechanical cooling.
Spatiotemporal Variations and Health Implications of Hazardous Air Pollutants in Ulsan, a Multi-Industrial City in Korea
Authors: Baek Kyung-Min , Kim Min-Ji , Seo Young-Kyo , Kang Byung-Wook , Kim Jong-Ho , Baek Sung-Ok
Published: May 2020

We measured a wide range of hazardous air pollutants (HAPs) simultaneously at five sites over four seasons in 2009–2010 in Ulsan, the largest industrial city in Korea. Target analytes included volatile organic compounds (VOCs), carbonyls, polycyclic aromatic hydrocarbons (PAHs), phthalates, and heavy metals (HMs). The objectives of this study were to evaluate the occurrence and spatiotemporal distributions of HAPs, and to identify important HAPs based on health risk assessment. Industrial emissions affected ambient levels of VOCs and HMs, as demonstrated by spatial distribution analysis. However, concentrations of PAHs and phthalates were relatively uniform at all sites. VOCs and HMs exhibited little seasonal variation, while formaldehyde increased in the summer due to its secondary formation. PAHs exhibited notable seasonal variation; higher in cold seasons and lower in warm seasons. Cumulative cancer risks imposed by 35 HAPs were 4.7 × 10−4 and 1.7 × 10−4 in industrial and residential areas, respectively. The top five major cancer risk drivers appeared to be formaldehyde, benzene, benzo[a]pyrene, As, and Co. The sums of hazard quotients (HQ) derived by 47 HAPs were 10.0 (industrial) and 2.4 (residential). As the individual species, only two HAPs exceeded the HQ of 1, which are As (3.1) and Pb (2.1) in the industrial area. This study demonstrated the importance of a comprehensive monitoring and health risk assessment to prioritize potentially toxic pollutants in the ambient air of a large industrial city.
On the Stability of the Generalized Psi Functional Equation
Authors: Kim Gwang Hui , Rassias Themistocles M.
Published: May 2020

In this paper, we investigate the generalized Hyers–Ulam stability for the generalized psi functional equation f ( x + p ) = f ( x ) + φ ( x ) by the direct method in the sense of P. Gǎvruta and the Hyers–Ulam–Rassias stability.
Viridistratins A−C, Antimicrobial and Cytotoxic Benzo[j]fluoranthenes from Stromata of Annulohypoxylon viridistratum (Hypoxylaceae, Ascomycota)
Authors: Becker Kevin , Wessel Anna-Charleen , Luangsa-ard J. Jennifer , Stadler Marc
Published: May 2020

During the course of our search for novel biologically active metabolites from tropical fungi, we are using chemotaxonomic and taxonomic methodology for the preselection of interesting materials. Recently, three previously undescribed benzo[j]fluoranthenes (1−3) together with the known derivatives truncatones A and C (4, 5) were isolated from the stromata of the recently described species Annulohypoxylon viridistratum collected in Thailand. Their chemical structures were elucidated by means of spectral methods, including nuclear magnetic resonance (NMR) spectroscopy and high-resolution mass spectrometry (HR-MS). The new compounds, for which we propose the trivial names viridistratins A−C, exhibited weak-to-moderate antimicrobial and cytotoxic activities in cell-based assays.
Novel Barbiturate-Nitrate Compounds Inhibit the Upregulation of Matrix Metalloproteinase-9 Gene Expression in Intestinal Inflammation through a cGMP-Mediated Pathway
Authors: O’Sullivan Shane , Wang Jun , Radomski Marek W. , Gilmer John F. , Medina Carlos
Published: May 2020

Matrix metalloproteinase-9 is upregulated in inflammatory bowel disease. Barbiturate nitrate hybrid compounds have been designed to inhibit MMP secretion and enzyme activity. In this study, we investigated the mechanism of action of barbiturate-nitrate hybrid compounds and their component parts using models of intestinal inflammation in vitro. Cytokine-stimulated Caco-2 cells were used in all in vitro experiments. The NO donors SNAP and DETA-NONOate were used to study the effect of NO on MMP-9 mRNA. Mechanistic elucidation was carried out using the soluble guanylate cyclase (sGC) inhibitor, ODQ, and the cGMP analogue, 8-Bromo-cGMP. Further experiments were carried out to elucidate the role of NF-κB. NO donors exerted an inhibitory effect on MMP-9 mRNA in cytokine-stimulated cells. While the non-nitrate barbiturates had a limited effect on MMP-9 expression, the hybrid compounds inhibited MMP-9 expression through its NO-mimetic properties. No effect could be observed on mRNA for MMP-1 or MMP-2. The sGC inhibitior, ODQ, abolished the nitrate-barbiturate inhibition of MMP-9 gene expression, an effect which was reversed by 8-Br-cGMP. This study shows that the barbiturate scaffold is suitable for hybrid design as an MMP-9 inhibitor in cytokine-stimulated Caco-2 cells. The inhibition of MMP-9 levels was largely mediated through a reduction in its mRNA by a sGC/cGMP pathway mediated mechanism.
Evaluation of Aptamers as Affinity Reagents for an Enhancement of SRM-Based Detection of Low-Abundance Proteins in Blood Plasma
Authors: Radko Sergey , Ptitsyn Konstantin , Novikova Svetlana , Kiseleva Yana , Moysa Alexander , Kurbatov Leonid , Mannanova Maria , Zgoda Victor , Ponomarenko Elena , Lisitsa Andrey , Archakov Alexander
Published: May 2020

Selected reaction monitoring (SRM) is a mass spectrometric technique characterized by the exceptionally high selectivity and sensitivity of protein detection. However, even with this technique, the quantitative detection of low- and ultralow-abundance proteins in blood plasma, which is of great importance for the search and verification of novel protein disease markers, is a challenging task due to the immense dynamic range of protein abundance levels. One approach used to overcome this problem is the immunoaffinity enrichment of target proteins for SRM analysis, employing monoclonal antibodies. Aptamers appear as a promising alternative to antibodies for affinity enrichment. Here, using recombinant protein SMAD4 as a model target added at known concentrations to human blood plasma and SRM as a detection method, we investigated a relationship between the initial amount of the target protein and its amount in the fraction enriched with SMAD4 by an anti-SMAD4 DNA-aptamer immobilized on magnetic beads. It was found that the aptamer-based enrichment provided a 30-fold increase in the sensitivity of SRM detection of SMAD4. These results indicate that the aptamer-based affinity enrichment of target proteins can be successfully employed to improve quantitative detection of low-abundance proteins by SRM in undepleted human blood plasma.
Comprehensive Exonic Sequencing of Known Ataxia Genes in Episodic Ataxia
Authors: Maksemous Neven , Sutherland Heidi G. , Smith Robert A. , Haupt Larisa M. , Griffiths Lyn R.
Published: May 2020

Episodic Ataxias (EAs) are a small group (EA1–EA8) of complex neurological conditions that manifest as incidents of poor balance and coordination. Diagnostic testing cannot always find causative variants for the phenotype, however, and this along with the recently proposed EA type 9 (EA9), suggest that more EA genes are yet to be discovered. We previously identified disease-causing mutations in the CACNA1A gene in 48% (n = 15) of 31 patients with a suspected clinical diagnosis of EA2, and referred to our laboratory for CACNA1A gene testing, leaving 52% of these cases (n = 16) with no molecular diagnosis. In this study, whole exome sequencing (WES) was performed on 16 patients who tested negative for CACNA1A mutations. Tiered analysis of WES data was performed to first explore (Tier-1) the ataxia and ataxia-associated genes (n = 170) available in the literature and databases for comprehensive EA molecular genetic testing; we then investigated 353 ion channel genes (Tier-2). Known and potential causal variants were identified in n = 8/16 (50%) patients in 8 genes (SCN2A, p.Val1325Phe; ATP1A3, p.Arg756His; PEX7, p.Tyr40Ter; and KCNA1, p.Arg167Met; CLCN1, p.Gly945ArgfsX39; CACNA1E, p.Ile614Val; SCN1B, p.Cys121Trp; and SCN9A, p.Tyr1217Ter). These results suggest that mutations in these genes might cause an ataxia phenotype or that combinations of more than one mutation contribute to ataxia disorders.
Ataxia as the Major Manifestation of Fragile X-Associated Tremor/Ataxia Syndrome (FXTAS): Case Series
Authors: Salcedo-Arellano Maria Jimena , Cabal-Herrera Ana Maria , Tassanakijpanich Nattaporn , McLennan Yingratana A. , Hagerman Randi J.
Published: May 2020

Fragile X-associated tremor and ataxia syndrome (FXTAS) is a neurodegenerative disease developed by carriers of a premutation in the fragile X mental retardation 1 (FMR1) gene. The core clinical symptoms usually manifest in the early 60s, typically beginning with intention tremor followed by cerebellar ataxia. Ataxia can be the only symptom in approximately 20% of the patients. FXTAS has a slow progression, and patients usually experience advanced deterioration 15 to 25 years after the initial diagnosis. Common findings in brain imaging include substantial brain atrophy and white matter disease (WMD). We report three cases with an atypical clinical presentation, all presenting with gait problems as their initial manifestation and with ataxia as the dominant symptom without significant tremor, as well as a faster than usual clinical progression. Magnetic resonance imaging (MRI) was remarkable for severe brain atrophy, ventriculomegaly, thinning of the corpus callosum, and periventricular WMD. Two cases were diagnosed with definite FXTAS on the basis of clinical and radiological findings, with one individual also developing moderate dementia. Factors such as environmental exposure and general anesthesia could have contributed to their clinical deterioration. FXTAS should be considered in the differential diagnosis of patients presenting with ataxia, even in the absence of tremor, and FMR1 DNA testing should be sought in those with a family history of fragile X syndrome or premutation disorders.