bluuebunny/crossref_metadata_2025 huggingface dataset:
Rapamycin Alternatively Modifies Mitochondrial Dynamics in Dendritic Cells to Reduce Kidney Ischemic Reperfusion Injury
Authors: Namwanje Maria , Bisunke Bijay , Rousselle Thomas V. , Lamanilao Gene G. , Sunder Venkatadri S. , Patterson Elizabeth C. , Kuscu Canan , Kuscu Cem , Maluf Daniel , Kiran Manjari , Mas Valeria , Eason James D. , Bajwa Amandeep
Published: May 2021

Dendritic cells (DCs) are unique immune cells that can link innate and adaptive immune responses and Immunometabolism greatly impacts their phenotype. Rapamycin is a macrolide compound that has immunosuppressant functions and is used to prevent graft loss in kidney transplantation. The current study evaluated the therapeutic potential of ex-vivo rapamycin treated DCs to protect kidneys in a mouse model of acute kidney injury (AKI). For the rapamycin single (S) treatment (Rapa-S-DC), Veh-DCs were treated with rapamycin (10 ng/mL) for 1 h before LPS. In contrast, rapamycin multiple (M) treatment (Rapa-M-DC) were exposed to 3 treatments over 7 days. Only multiple ex-vivo rapamycin treatments of DCs induced a persistent reprogramming of mitochondrial metabolism. These DCs had 18-fold more mitochondria, had almost 4-fold higher oxygen consumption rates, and produced more ATP compared to Veh-DCs (Veh treated control DCs). Pathway analysis showed IL10 signaling as a major contributing pathway to the altered immunophenotype after Rapamycin treatment compared to vehicle with significantly lower cytokines Tnfa, Il1b, and Il6, while regulators of mitochondrial content Pgc1a, Tfam, and Ho1 remained elevated. Critically, adoptive transfer of rapamycin-treated DCs to WT recipients 24 h before bilateral kidney ischemia significantly protected the kidneys from injury with a significant 3-fold improvement in kidney function. Last, the infusion of DCs containing higher mitochondria numbers (treated ex-vivo with healthy isolated mitochondria (10 µg/mL) one day before) also partially protected the kidneys from IRI. These studies demonstrate that pre-emptive infusion of ex-vivo reprogrammed DCs that have higher mitochondria content has therapeutic capacity to induce an anti-inflammatory regulatory phenotype to protect kidneys from injury.
Vascular Endothelial Growth Factor Biology and Its Potential as a Therapeutic Target in Rheumatic Diseases
Authors: Le Thi Hong Van , Kwon Sang-Mo
Published: May 2021

Rheumatic diseases constitute a diversified group of diseases distinguished by arthritis and often involve other organs. The affected individual has low quality of life, productivity even life-threatening in some severe conditions. Moreover, they impose significant economic and social burdens. In recent years, the patient outcome has been improved significantly due to clearer comprehension of the pathology of rheumatic diseases and the effectiveness of “treat to target” therapies. However, the high cost and the adverse effects are the concerns and full remissions are not often observed. One of the main processes that contributes to the pathogenesis of rheumatic diseases is angiogenesis. Vascular endothelial growth factor (VEGF), a central mediator that regulates angiogenesis, has different isoforms and functions in various physiological processes. Increasing evidence suggests an association between the VEGF system and rheumatic diseases. Anti-VEGF and VEGF receptor (VEGFR) therapies have been used to treat several cancers and eye diseases. This review summarizes the current understanding of VEGF biology and its role in the context of rheumatic diseases, the contribution of VEGF bioavailability in the pathogenesis of rheumatic diseases, and the potential implications of therapeutic approaches targeting VEGF for these diseases.
Partial Deficiency of Zfp217 Resists High-Fat Diet-Induced Obesity by Increasing Energy Metabolism in Mice
Authors: Zeng Qianhui , Wang Nannan , Zhang Yaru , Yang Yuxuan , Li Shuangshuang , Zheng Rong , Chai Jin , Qiao Tong , Jiang Siwen
Published: May 2021

Obesity-induced adipose tissue dysfunction and disorders of glycolipid metabolism have become a worldwide research priority. Zfp217 plays a crucial role in adipogenesis of 3T3-L1 preadipocytes, but about its functions in animal models are not yet clear. To explore the role of Zfp217 in high-fat diet (HFD)-induced obese mice, global Zfp217 heterozygous knockout (Zfp217+/−) mice were constructed. Zfp217+/− mice and Zfp217+/+ mice fed a normal chow diet (NC) did not differ significantly in weight gain, percent body fat mass, glucose tolerance, or insulin sensitivity. When challenged with HFD, Zfp217+/− mice had less weight gain than Zfp217+/+ mice. Histological observations revealed that Zfp217+/− mice fed a high-fat diet had much smaller white adipocytes in inguinal white adipose tissue (iWAT). Zfp217+/− mice had improved metabolic profiles, including improved glucose tolerance, enhanced insulin sensitivity, and increased energy expenditure compared to the Zfp217+/+ mice under HFD. We found that adipogenesis-related genes were increased and metabolic thermogenesis-related genes were decreased in the iWAT of HFD-fed Zfp217+/+ mice compared to Zfp217+/− mice. In addition, adipogenesis was markedly reduced in mouse embryonic fibroblasts (MEFs) from Zfp217-deleted mice. Together, these data indicate that Zfp217 is a regulator of energy metabolism and it is likely to provide novel insight into treatment for obesity.
SUMO-Targeted Ubiquitin Ligases and Their Functions in Maintaining Genome Stability
Authors: Chang Ya-Chu , Oram Marissa K. , Bielinsky Anja-Katrin
Published: May 2021

Small ubiquitin-like modifier (SUMO)-targeted E3 ubiquitin ligases (STUbLs) are specialized enzymes that recognize SUMOylated proteins and attach ubiquitin to them. They therefore connect the cellular SUMOylation and ubiquitination circuits. STUbLs participate in diverse molecular processes that span cell cycle regulated events, including DNA repair, replication, mitosis, and transcription. They operate during unperturbed conditions and in response to challenges, such as genotoxic stress. These E3 ubiquitin ligases modify their target substrates by catalyzing ubiquitin chains that form different linkages, resulting in proteolytic or non-proteolytic outcomes. Often, STUbLs function in compartmentalized environments, such as the nuclear envelope or kinetochore, and actively aid in nuclear relocalization of damaged DNA and stalled replication forks to promote DNA repair or fork restart. Furthermore, STUbLs reside in the same vicinity as SUMO proteases and deubiquitinases (DUBs), providing spatiotemporal control of their targets. In this review, we focus on the molecular mechanisms by which STUbLs help to maintain genome stability across different species.
α-Synuclein A53T Binds to Transcriptional Adapter 2-Alpha and Blocks Histone H3 Acetylation
Authors: Lee Ji-Yeong , Kim Hanna , Jo Areum , Khang Rin , Park Chi-Hu , Park Soo-Jeong , Kwag Eunsang , Shin Joo-Ho
Published: May 2021

α-Synuclein (α-syn) is a hallmark amyloidogenic protein component of Lewy bodies in dopaminergic neurons affected by Parkinson’s disease (PD). Despite the multi-faceted gene regulation of α-syn in the nucleus, the mechanism underlying α-syn crosstalk in chromatin remodeling in PD pathogenesis remains elusive. Here, we identified transcriptional adapter 2-alpha (TADA2a) as a novel binding partner of α-syn using the BioID system. TADA2a is a component of the p300/CBP-associated factor and is related to histone H3/H4 acetylation. We found that α-syn A53T was more preferentially localized in the nucleus than the α-syn wild-type (WT), leading to a stronger disturbance of TADA2a. Indeed, α-syn A53T significantly reduced the level of histone H3 acetylation in SH-SY5Y cells; its reduction was also evident in the striatum (STR) and substantia nigra (SN) of mice that were stereotaxically injected with α-syn preformed fibrils (PFFs). Interestingly, α-syn PFF injection resulted in a decrease in TADA2a in the STR and SN of α-syn PFF-injected mice. Furthermore, the levels of TADA2a and acetylated histone H3 were significantly decreased in the SN of patients with PD. Therefore, histone modification through α-syn A53T-TADA2a interaction may be associated with α-syn-mediated neurotoxicity in PD pathology.
CRL4-DCAF12 Ubiquitin Ligase Controls MOV10 RNA Helicase during Spermatogenesis and T Cell Activation
Authors: Lidak Tomas , Baloghova Nikol , Korinek Vladimir , Sedlacek Radislav , Balounova Jana , Kasparek Petr , Cermak Lukas
Published: May 2021

Multisubunit cullin-RING ubiquitin ligase 4 (CRL4)-DCAF12 recognizes the C-terminal degron containing acidic amino acid residues. However, its physiological roles and substrates are largely unknown. Purification of CRL4-DCAF12 complexes revealed a wide range of potential substrates, including MOV10, an “ancient” RNA-induced silencing complex (RISC) complex RNA helicase. We show that DCAF12 controls the MOV10 protein level via its C-terminal motif in a proteasome- and CRL-dependent manner. Next, we generated Dcaf12 knockout mice and demonstrated that the DCAF12-mediated degradation of MOV10 is conserved in mice and humans. Detailed analysis of Dcaf12-deficient mice revealed that their testes produce fewer mature sperms, phenotype accompanied by elevated MOV10 and imbalance in meiotic markers SCP3 and γ-H2AX. Additionally, the percentages of splenic CD4+ T and natural killer T (NKT) cell populations were significantly altered. In vitro, activated Dcaf12-deficient T cells displayed inappropriately stabilized MOV10 and increased levels of activated caspases. In summary, we identified MOV10 as a novel substrate of CRL4-DCAF12 and demonstrated the biological relevance of the DCAF12-MOV10 pathway in spermatogenesis and T cell activation.
Clinical Characteristics of POC1B-Associated Retinopathy and Assignment of Pathogenicity to Novel Deep Intronic and Non-Canonical Splice Site Variants
Authors: Weisschuh Nicole , Mazzola Pascale , Bertrand Miriam , Haack Tobias B. , Wissinger Bernd , Kohl Susanne , Stingl Katarina
Published: May 2021

Mutations in POC1B are a rare cause of inherited retinal degeneration. In this study, we present a thorough phenotypic and genotypic characterization of three individuals harboring putatively pathogenic variants in the POC1B gene. All patients displayed a similar, slowly progressive retinopathy (cone dystrophy or cone-rod dystrophy) with normal funduscopy but disrupted outer retinal layers on optical coherence tomography and variable age of onset. Other symptoms were decreased visual acuity and photophobia. Whole genome sequencing revealed a novel homozygous frameshift variant in one patient. Another patient was shown to harbor a novel deep intronic variant in compound heterozygous state with a previously reported canonical splice site variant. The third patient showed a novel nonsense variant and a novel non-canonical splice site variant. We aimed to validate the effect of the deep intronic variant and the non-canonical splice site variant by means of in vitro splice assays. In addition, direct RNA analysis was performed in one patient. Splicing analysis revealed that the non-canonical splice site variant c.561-3T>C leads to exon skipping while the novel deep intronic variant c.1033-327T>A causes pseudoexon activation. Our data expand the genetic landscape of POC1B mutations and confirm the benefit of genome sequencing in combination with downstream functional validation using minigene assays for the analysis of putative splice variants. In addition, we provide clinical multimodal phenotyping of the affected individuals.
Effect of Processed Beverage By-Product-Based Diets on Biological Parameters, Conversion Efficiency and Body Composition of Hermetia illucens (L) (Diptera: Stratiomyidae)
Authors: Sideris Vassilios , Georgiadou Maria , Papadoulis Georgios , Mountzouris Konstantinos , Tsagkarakis Antonios
Published: May 2021

The effect of spent coffee grounds (SCG), brewer’s spent grains (BSG) and their mixtures with the addition of brewer’s yeast (BY) were tested in two rearing densities of the Black Soldier Fly, Hermetia illucens (L.). Different treatments were investigated on larval development, survival, yield, protein conversion (PrCR) and bioconversion rate (BCR), substrate mass reduction and body composition of the insect. BSF larvae were able to develop sufficiently in all diets, except on sole SCG. The addition of BY enhanced the performance properties of diets, especially in the case of SCG, where larvae underperformed. Substrate mass reduction, PrCR and BCR were affected only by feed and exhibited higher values on reference feed, followed by BSG and SCG+BSG enriched with BY. Density did not have a significant effect on various larval nutrients, except for fat, which was higher on larvae fed enriched feeds with BY and in the 300 larval density. The interaction between feed and density strongly affected the nitrogen and protein levels, larval yield and ash. Generally, diets which contained SCG exhibited high larval crude protein levels. Our results illustrate that low value beverage by-products can be successfully utilized as constituents of a successful BSF diet.
Evaluation of Ethanol Extract of Moringa oleifera Lam. as Acaricide against Oligonychus punicae Hirst (Trombidiformes: Tetranychidae)
Authors: Heinz-Castro Rapucel Tonantzin Quetzalli , Arredondo-Valdés Roberto , Ordaz-Silva Salvador , Méndez-Cortés Heriberto , Hernández-Juárez Agustín , Chacón-Hernández Julio César
Published: May 2021

Tetranychidae family is a major group of mites causing serious damage in agricultural, vegetable and ornamental crops. Avocado bronze mite (ABM), Oligonychus punicae Hirst (Acari: Tetranychidae) causes major crop damage, defoliation and fruit abortion. At present, the control of this mite depends mainly on agrochemicals. Therefore it is necessary to find alternatives to synthetic pesticides that can help minimize environmental impact and health risks for the consumers. The aim of this research was to assess the effect of different concentrations (0.1, 0.5, 1, 5, 10, 15 and 20% (v/v)) of ethanolic extract of Moringa oleifera leaves against adult ABM females. Mites treated with 0.1 and 20% (v/v) of the extract showed mortality of 0.00% and 46.67%, 6.67% and 86.67%, 13.70% and 96.67%, at 24, 48 and 72 h, as compared to the control treatment, respectively. The number of eggs laid and hatch, as well as ABM feeding rates, depended on the extract concentration, which led to a reduction in the growth rate. M. oleifera leaf ethanolic extract has potential to control O. punicae.
Findings of Brain Magnetic Resonance Imaging in Girls with Central Precocious Puberty Compared with Girls with Chronic or Recurrent Headache
Authors: Kim Shin-Hee , Ahn Moon Bae , Cho Won Kyoung , Cho Kyoung Soon , Jung Min Ho , Suh Byung-Kyu
Published: May 2021

In the present study, the results of brain magnetic resonance imaging (MRI) in girls with central precocious puberty (CPP) were compared those in with girls evaluated for headaches. A total of 295 girls with CPP who underwent sellar MRI were enrolled. A total of 205 age-matched girls with chronic or recurrent headaches without neurological abnormality who had brain MRI were included as controls. The positive MRI findings were categorized as incidental non-hypothalamic–pituitary (H–P), incidental H–P, or pathological. Positive MRI findings were observed in 39 girls (13.2%) with CPP; 8 (2.7%) were classified as incidental non-H–P lesions, 30 (10.2%) as incidental H–P lesions, and 1 (0.3%) as a pathological lesion (tuber cinereum hamartoma). The prevalence of positive MRI findings in girls with CPP did not differ from girls with headaches (13.2% vs. 12.2%, p = 0.74). The prevalence of incidental H–P lesions in girls with CPP <6 years of age, 6–6.9 years of age, and 7–7.9 years of age was 21.2%, 13.5%, and 9.6%, respectively (p = 0.21). Known pathological lesions were detected in only one (3.0%) girl with CPP aged <6 years and in no girls with CPP aged 6–7.9 years. Microadenomas were detected in no girls with CPP aged <6 years and in 5 (1.9%) girls with CPP aged of 6–7.9 years. Our findings call into question the routine use of brain MRI in girls with CPP, especially in girls 6 years or older. Current guidelines recommend a follow-up MRI in cases of microadenoma, but few data exist to support this recommendation for children.
Association of GRACE Risk Score with Coronary Artery Disease Complexity in Patients with Acute Coronary Syndrome
Authors: Sofidis Georgios , Otountzidis Nikolaos , Stalikas Nikolaos , Karagiannidis Efstratios , Papazoglou Andreas S. , Moysidis Dimitrios V. , Panteris Eleftherios , Deda Olga , Kartas Anastasios , Zegkos Thomas , Daskalaki Paraskevi , Theodoridou Niki , Stefanopoulos Leandros , Karvounis Haralambos , Gika Helen , Theodoridis Georgios , Sianos Georgios
Published: May 2021

The GRACE score constitutes a useful tool for risk stratification in patients with acute coronary syndrome (ACS), while the SYNTAX score determines the complexity of coronary artery disease (CAD). This study sought to correlate these scores and assess the accuracy of the GRACE score in predicting the extent of CAD. A total of 539 patients with ACS undergoing coronary angiography were included in this analysis. The patients were classified into those with a SYNTAX score < 33 and a SYNTAX score ≥ 33. Spearman’s correlation and receiver operator characteristic analysis were conducted to investigate the role of the GRACE score as a predictor of the SYNTAX score. There was a significantly positive correlation between the SYNTAX and the GRACE scores (r = 0.32, p < 0.001). The GRACE score predicted severe CAD (SYNTAX ≥ 33) moderately well (the area under the curve was 0.595 (0.522–0.667)). A GRACE score of 126 was documented as the optimal cut-off for the prediction of a SYNTAX score ≥ 33 (sensitivity = 53.5% and specificity = 66%). Therefore, our study reports a significantly positive correlation between the GRACE and the SYNTAX score in patients with ACS. Notably, NSTEMI patients with a high-risk coronary anatomy have higher calculated GRACE scores. A multidisciplinary approach by a heart team could possibly alter the therapeutic approach and management in patients presenting with ACS and a high calculated GRACE score.
Risk Categories in COVID-19 Based on Degrees of Inflammation: Data on More Than 17,000 Patients from the Spanish SEMI-COVID-19 Registry
Authors: Rubio-Rivas Manuel , Corbella Xavier , Formiga Francesc , Menéndez Fernández Estela , Martín Escalante María , Baños Fernández Isolina , Arnalich Fernández Francisco , Del Corral-Beamonte Esther , Lalueza Antonio , Parra Virto Alejandro , Roy Vallejo Emilia , Loureiro-Amigo José , Álvarez Suárez Ana , Abadía-Otero Jesica , Navarro De La Chica María , Estévez González Raquel , Hernández Milián Almudena , Areses Manrique María , Blázquez Encinar Julio , González Noya Amara , González Ferrer Ruth , Pérez Aguilera María , Gil Sánchez Ricardo , Millán Núñez-Cortés Jesús , Casas-Rojo José ,   on behalf of the SEMI-COVID-19 Network
Published: May 2021

(1) Background: The inflammation or cytokine storm that accompanies COVID-19 marks the prognosis. This study aimed to identify three risk categories based on inflammatory parameters on admission. (2) Methods: Retrospective cohort study of patients diagnosed with COVID-19, collected and followed-up from 1 March to 31 July 2020, from the nationwide Spanish SEMI-COVID-19 Registry. The three categories of low, intermediate, and high risk were determined by taking into consideration the terciles of the total lymphocyte count and the values of C-reactive protein, lactate dehydrogenase, ferritin, and D-dimer taken at the time of admission. (3) Results: A total of 17,122 patients were included in the study. The high-risk group was older (57.9 vs. 64.2 vs. 70.4 years; p < 0.001) and predominantly male (37.5% vs. 46.9% vs. 60.1%; p < 0.001). They had a higher degree of dependence in daily tasks prior to admission (moderate-severe dependency in 10.8% vs. 14.1% vs. 17%; p < 0.001), arterial hypertension (36.9% vs. 45.2% vs. 52.8%; p < 0.001), dyslipidemia (28.4% vs. 37% vs. 40.6%; p < 0.001), diabetes mellitus (11.9% vs. 17.1% vs. 20.5%; p < 0.001), ischemic heart disease (3.7% vs. 6.5% vs. 8.4%; p < 0.001), heart failure (3.4% vs. 5.2% vs. 7.6%; p < 0.001), liver disease (1.1% vs. 3% vs. 3.9%; p = 0.002), chronic renal failure (2.3% vs. 3.6% vs. 6.7%; p < 0.001), cancer (6.5% vs. 7.2% vs. 11.1%; p < 0.001), and chronic obstructive pulmonary disease (5.7% vs. 5.4% vs. 7.1%; p < 0.001). They presented more frequently with fever, dyspnea, and vomiting. These patients more frequently required high flow nasal cannula (3.1% vs. 4.4% vs. 9.7%; p < 0.001), non-invasive mechanical ventilation (0.9% vs. 3% vs. 6.3%; p < 0.001), invasive mechanical ventilation (0.6% vs. 2.7% vs. 8.7%; p < 0.001), and ICU admission (0.9% vs. 3.6% vs. 10.6%; p < 0.001), and had a higher percentage of in-hospital mortality (2.3% vs. 6.2% vs. 23.9%; p < 0.001). The three risk categories proved to be an independent risk factor in multivariate analyses. (4) Conclusion: The present study identifies three risk categories for the requirement of high flow nasal cannula, mechanical ventilation, ICU admission, and in-hospital mortality based on lymphopenia and inflammatory parameters.
Physico-Chemical Properties and Biocompatibility of Thermosensitive Chitosan Lactate and Chitosan Chloride Hydrogels Developed for Tissue Engineering Application
Authors: Pieklarz Katarzyna , Galita Grzegorz , Tylman Michał , Maniukiewicz Waldemar , Kucharska Ewa , Majsterek Ireneusz , Modrzejewska Zofia
Published: May 2021

Recently, the modification of the initial structure of biopolymers, mainly chitosan, has been gaining importance with a view to obtain functional forms with increased practicality and specific properties enabling their use in tissue engineering. Therefore, in this article, the properties (structural and biological) of thermosensitive hydrogels obtained from chitosan lactate/chloride and two types of crosslinking agents (β-glycerol phosphate disodium salt pentahydrate and uridine 5′-monophosphate disodium salt) are discussed. The aim of the research is to identify changes in the structure of the biomaterials during conditioning in water. Structural investigations were carried out by FTIR spectroscopy. The crystallinity of gels was determined by X-ray diffraction analysis. The biocompatibility (evaluation of cytotoxicity and genotoxicity) of chitosan hydrogels was investigated by contact with human colon adenocarcinoma cell line for 48 h. The cytotoxicity was verified based on the colorimetric resazurin assay, and the genotoxicity was checked by the comet assay (percentage of DNA in the comet tail). The conducted research showed that the analyzed types of chitosan hydrogels are non-cytotoxic and non-genotoxic materials. The good biocompatibility of chitosan hydrogels surfaces makes them interesting scaffolds with clinical potential in tissue regeneration engineering.
Transcriptome Analysis Identifies a Gene Cluster for the Biosynthesis of Biruloquinone, a Rare Phenanthraquinone, in a Lichen-Forming Fungus Cladonia macilenta
Authors: Kim Wonyong , Jeong Min-Hye , Yun Sung-Hwan , Hur Jae-Seoun
Published: May 2021

Lichens are prolific producers of natural products of polyketide origin. We previously described a culture of lichen-forming fungus (LFF) Cladonia macilenta that produces biruloquinone, a purple pigment that is a phenanthraquinone rarely found in nature. However, there was no genetic information on the biosynthesis of biruloquinone. To identify a biosynthetic gene cluster for biruloquinone, we mined polyketide synthase (PKS) genes from the genome sequence of a LFF isolated from thalli of C. macilenta. The 38 PKS in C. macilenta are highly diverse, many of which form phylogenetic clades with PKS previously characterized in non-lichenized fungi. We compared transcriptional profiles of the 38 PKS genes in two chemotypic variants, one producing biruloquinone and the other producing no appreciable metabolite in vitro. We identified a PKS gene (hereafter PKS21) that was highly upregulated in the LFF that produces biruloquinone. The boundaries of a putative biruloquinone gene cluster were demarcated by co-expression patterns of six clustered genes, including the PKS21. Biruloquinone gene clusters exhibited a high degree of synteny between related species. In this study we identified a novel PKS family responsible for the biosynthesis of biruloquinone through whole-transcriptome analysis.
Rethinking of the Roles of Endophyte Symbiosis and Mycotoxin in Oxytropis Plants
Authors: Guan Huirui , Liu Xin , Mur Luis A. J. , Fu Yanping , Wei Yahui , Wang Jing , He Wei
Published: May 2021

Plants in the Oxytropis genus can live with the endophytic fungi Alternaria sect. Undifilum. Swainsonine, the mycotoxin produced by the endophyte render the host plant toxic and this has been detrimental to grazing livestock in both China and U.S.A. Despite previous efforts, many questions remain to be solved, such as the transmission mode and life cycle of host–endophyte symbiont, the biosynthesis pathway of swainsonine, and in particular the ecological role and evolution of such symbiosis. In this review, we compile the literature to synthesize ideas on the diversity of the symbiosis and propagation of the endophyte. We further compare the previous work from both Alternaria sect. Undifilum and other swainsonine producing fungi to orchestrate a more comprehensive biosynthesis pathway of swainsonine. We also connect swainsonine biosynthesis pathway with that of its precursor, lysine, and link this to a potential role in modulating plant stress response. Based on this we hypothesize that this host–endophyte co-evolution originated from the needs for host plant to adapt for stress. Validation of this hypothesis will depend on future research on endophytic symbiosis in Oxytropis and help in better understanding the roles of plant–endophyte symbiosis in non-Poaceae species.
Complications after Thoracocentesis and Chest Drain Insertion: A Single Centre Study from the North East of England
Authors: Jackson Karl , Kafi Opeyemi , Bhullar Dilraj S. , Scott Jordan , Storey Claire , Hyatali Saara , Carlin Hannah , Brown Andrew , Grimshaw Emily , Miller Joseph , Rank Hannah , Porritt Sean , Carling Michael , Aujayeb Avinash
Published: May 2021

Introduction: There are no prospective studies looking at complications of pleural procedures. Previous British Thoracic Society Pleural audits and retrospective case series inform current practice. Incidence of any complication is between 1–15%. We sought to add to the existing literature and inform local practice with regards to intercostal drains and thoracocenteses. Methods: Local Caldicott approval was sought for a review of all inpatient adult pleural procedures coded as ‘T122 drainage of pleural cavity’ and ‘T124 insertion of tube drain into pleural cavity’. Those undergoing thoracocentesis (all with a Rocket 6 Fg catheter) and intercostal drain insertion (ICD, all with Rocket 12 Fg drain) were identified. Continuous variables are presented as mean (±range) and categorical variables as percentages where appropriate. Results: 1159 procedures were identified. A total of 199 and 960 were done for pneumothorax and effusions respectively. Mean age was 68.1 years (18–97). There were 280 thoracocenteses and 879 ICDs. Bleeding occurred in 6 (0.5%), all ICDs (clotting and platelets were within normal range; one patient was on aspirin and one on aspirin and clopidogrel). All settled except for one who had intercostal artery rupture needing cardiothoracic intervention (no anti-coagulation). Nine pneumothoraces occurred (0.78%) in seven ICDs and two aspirations). There were three definite pleural space infections (0.3%) with three ICDs. Fall out rates for ICDs were 35 (3%). Nine were not sutured, and out of those, seven inserted in the Accident and Emergency department, out of hours. All others ‘came out’ due to patient factors (previous quoted rates up to 14%). Surgical emphysema occurred in 43 (41 ICDs), 3.7%. Eight were due to fall outs and three required surgical intervention. There was no re-expansion pulmonary oedema nor direct deaths. Conclusions: Complication rates of ICD and thoracocenteses are low. Checklists might help to remind operators of the need for suturing. Limitations of this study are its retrospective nature and reliance on correct hospital coding. We are currently contributing to a prospective observational study on pleural complications.
Human Body Rhythms in the Development of Non-Invasive Methods of Closed-Loop Adaptive Neurostimulation
Authors: Fedotchev Alexander , Parin Sergey , Polevaya Sofia , Zemlianaia Anna
Published: May 2021

The creation and improvement of non-invasive closed-loop brain stimulation technologies represent an exciting and rapidly expanding field of neuroscience. To identify the appropriate way to close the feedback loop in adaptive neurostimulation procedures, it was previously proposed to use on-line automatic sensory stimulation with the parameters modulated by the patient’s own rhythmical processes, such as respiratory rate, heart rate, and electroencephalogram (EEG) rhythms. The current paper aims to analyze several recent studies demonstrating further development in this line of research. The advantages of using automatic closed-loop feedback from human endogenous rhythms in non-invasive adaptive neurostimulation procedures have been demonstrated for relaxation assistance, for the correction of stress-induced functional disturbances, for anxiety management, and for the cognitive rehabilitation of an individual. Several distinctive features of the approach are noted to delineate its further development.
Understanding Neuromuscular Health and Disease: Advances in Genetics, Omics, and Molecular Function
Authors: Duddy William J. , Duguez Stephanie
Published: May 2021

The field of neuromuscular research has seen considerable recent advances in the molecular and cellular understanding of muscle biology, and the treatment of neuromuscular disease [...]
Do You See It Clearly? The Effect of Packaging and Label Format on Google Ads
Authors: Gómez-Carmona Diego , Cruces-Montes Serafín , Marín-Dueñas Pedro Pablo , Serrano-Domínguez César , Paramio Alberto , García Antonio Zayas
Published: May 2021

The nature of e-commerce prevents the perception of the intrinsic and sensory attributes of wine. In the virtual environment, visual cues allow consumers to perceive the product, determine their attitude and form a preference. Users will choose one product or another based on the visual appeal of the advertisements they have seen. Wine marketers must consider the importance of the advertisement elements and attract the consumer’s attention. Optimizing the elements included in these messages can help capture consumers’ attention and achieve a higher click-through rate on the ads. The main objective of this work is to analyse the awareness that different advertisements achieve. Specifically, we use a 2 × 2 × 2 experimental design where we manipulate the packaging format (single bottle vs. pack), labelling (bottle without label vs. labelled bottle) for wine ads (white and red). To analyse attention, we used an eye-tracking methodology. The main results suggest that attention is captured more quickly with an individual bottle without a label than with a particular bottle with a label in Google ads. However, ads showing packs of bottles with labels get more attention than ads using packs of bottles without labels.
The Spatial Effect of Administrative Division on Land-Use Intensity
Authors: Wang Pengrui , Zeng Chen , Song Yan , Guo Long , Liu Wenping , Zhang Wenting
Published: May 2021

Land-use intensity (LUI) is one of the most direct manifestations of regional land use efficiency. The study of cross-administrative LUI in urban agglomerations is of great importance for the sustainable development of land, new urbanization, and territorial spatial planning. In this study, the urban agglomeration in the middle reaches of the Yangtze River in China was used as the case study area to explore the spatial spillover effect through the administrative division, underlying driving mechanism, and spatial interactions or constraints of LUI. First, LUI was measured using the index of the proportion of construction land to the total area of the administrative region. Second, the adjacency relationship of the county-level administrative units was identified on the basis of the queen-type adjacency criterion under the county-level administrative division system. Thereafter, spatial weight matrix for spatial modeling was constructed. Last, a spatial model using the “Spatial adjacency matrix” was devised to examine the influencing factors and the potential spatial interactions or constraints of administrative units. Results revealed that the level of LUI of different county-level administrative units were quite different, and the gap of LUI among county-level administrative units widened from 2010 to 2017. The fixed asset investment per land (FAIL), gross domestic product per capital (PGDP), and proportion of tertiary sector (PTS) are the driving factors of LUI. County-level administrative units not only had a significant and increasing spatial interaction effect based on the relationship of cooperation, but also had an influence of restraint mutually which was caused by the competition. The direct spatial spillover effect was remarkable. In the future, the effect of interaction among administrative units under the administrative division should be considered to promote the reasonable use and optimal layout of regional urban land to realize the optimal allocation of land resources.
A Comprehensive Review of Urban Regeneration Governance for Developing Appropriate Governance Arrangements
Authors: Xie Fangyun , Liu Guiwen , Zhuang Taozhi
Published: May 2021

Urban regeneration governance (URG) has become a popular issue in academia, politics and civil society because it has a significant influence on the success of urban regeneration activities. However, a comprehensive review on URG has yet to be produced, which hinders providing references to developing appropriate governance arrangements. Therefore, this study selected 88 relevant literatures from 1990 to 2019 to conduct a critical review. The goal of this review is to conceptualize URG, refine the signature elements of URG, compare the main modes of URG, and analyze the influential factors of URG. As a decision-making mode or a partnership, URG consists of three elements—partner, power and procedure—and influenced by three factors, the plan, place and person. There are three main modes of URG and each has pros and cons. Based on a comprehensive review, this paper concludes some findings and draws an 8p model that can provide an analysis framework for decision makers. Finally, four avenues for future research are proposed.
Networking in the Time of COVID
Authors: Militello Jacqueline
Published: May 2021

This study examines how during COVID professionals in the financial sector in Hong Kong experienced adaptations to previous ways of networking and what the material outcomes were. Becoming acquainted traditionally relies heavily on face-to-face interaction to advance and cement feelings of trust that eventually lead to successfully concluded transactions. Using linguistic ethnography, I interviewed 36 professionals about networking during COVID. For all three aspects of networking (creating, cultivating, and utilizing relationships for attaining professional goals), participants indicated significant changes as embodied co-present interactions all but ceased and were replaced by computer-mediated communication, including video platforms such as Zoom. Many, but not all, participants indicated that they had made either no new, or a greatly decreased number of new professional acquaintances, compared to pre-COVID times. The cues that would be present in face-to-face interaction were largely viewed as essential for establishing trust in deepening relationships and achieving professional goals such as concluding transactions. There were some compensatory affordances such as more ‘objective’ evaluations and equalization for those in more peripheral geographic locations. The material outcomes were that, for most, new relationships were significantly handicapped, resulting in networks in a state of stasis, a situation that privileged extant connections and those with strong professional networks.
Threats to Women/Women as Threats: Male Supremacy and the Anti-Statist Right
Authors: Ebin Chelsea
Published: May 2021

Throughout the Trump administration, media coverage of extremist factions of the American right grew considerably, as did the actual membership and numbers of those factions. Included among these factions, and operating on a spectrum that ranges from the center-to-fringe right, are white supremacist, Christian nationalist, and militia/patriot/sovereign citizen (broadly termed constitutionalist) movements. While the American right is heterogeneous, most of these groups are composed of white men, and male supremacism is often a common ideological denominator. Based on historical trends, recent activity, and ongoing movement mobilizations, we should anticipate increased recruitment and activism on the part of anti-statist right-wing groups during the Biden administration. While much has been written about the threat of terroristic violence these groups pose and their varying levels of engagement with white supremacist beliefs, examinations of gender have largely focused on masculinity. This note takes up the relationship between anti-statist right-wing movements and women by sketching three key areas that warrant further examination: (1) how collective interpretations of the law leave women vulnerable by refusing the legitimacy of federal legislation; (2) the threat of militia violence against women, particularly those who hold elected office; (3) how racial and gender exclusions preclude women from having their claims to membership in anti-statist right-wing movements be fully recognized. As we take stock of the growing threat posed by these movements, it is incumbent on us to critically examine the threats to women’s rights posed by the anti-statist right.
The Use of Response Surface Methodology as a Statistical Tool for the Optimisation of Waste and Pure Canola Oil Biodegradation by Antarctic Soil Bacteria
Authors: Zahri Khadijah Nabilah Mohd , Zulkharnain Azham , Gomez-Fuentes Claudio , Sabri Suriana , Abdul Khalil Khalilah , Convey Peter , Ahmad Siti Aqlima
Published: May 2021

Hydrocarbons can cause pollution to Antarctic terrestrial and aquatic ecosystems, both through accidental release and the discharge of waste cooking oil in grey water. Such pollutants can persist for long periods in cold environments. The native microbial community may play a role in their biodegradation. In this study, using mixed native Antarctic bacterial communities, several environmental factors influencing biodegradation of waste canola oil (WCO) and pure canola oil (PCO) were optimised using established one-factor-at-a-time (OFAT) and response surface methodology (RSM) approaches. The factors include salinity, pH, type of nitrogen and concentration, temperature, yeast extract and initial substrate concentration in OFAT and only the significant factors proceeded for the statistical optimisation through RSM. High concentration of substrate targeted for degradation activity through RSM compared to OFAT method. As for the result, all factors were significant in PBD, while only 4 factors were significant in biodegradation of PCO (pH, nitrogen concentration, yeast extract and initial substrate concentration). Using OFAT, the most effective microbial community examined was able to degrade 94.42% and 86.83% (from an initial concentration of 0.5% (v/v)) of WCO and PCO, respectively, within 7 days. Using RSM, 94.99% and 79.77% degradation of WCO and PCO was achieved in 6 days. The significant interaction for the RSM in biodegradation activity between temperature and WCO concentration in WCO media were exhibited. Meanwhile, in biodegradation of PCO the significant factors were between (1) pH and PCO concentration, (2) nitrogen concentration and yeast extract, (3) nitrogen concentration and PCO concentration. The models for the RSM were validated for both WCO and PCO media and it showed no significant difference between experimental and predicted values. The efficiency of canola oil biodegradation achieved in this study provides support for the development of practical strategies for efficient bioremediation in the Antarctic environment.
A Digital Twin for Friction Prediction in Dynamic Rubber Applications with Surface Textures
Authors: Zambrano Valentina , Brase Markus , Hernández-Gascón Belén , Wangenheim Matthias , Gracia Leticia A. , Viejo Ismael , Izquierdo Salvador , Valdés José Ramón
Published: May 2021

Surface texturing is an effective method to reduce friction without the need to change materials. In this study, surface textures were transferred to rubber samples in the form of dimples, using a novel laser surface texturing (LST)—based texturing during moulding (TDM) production process, developed within the European Project MouldTex. The rubber samples were used to experimentally determine texture-induced friction variations, although, due to the complexity of manufacturing, only a limited amount was available. The tribological friction measurements were hence combined with an artificial intelligence (AI) technique, i.e., Reduced Order Modelling (ROM). ROM allows obtaining a virtual representation of reality through a set of numerical strategies for problem simplification. The ROM model was created to predict the friction outcome under different operating conditions and to find optimised dimple parameters, i.e., depth, diameter and distance, for friction reduction. Moreover, the ROM model was used to evaluate the impact on friction when manufacturing deviations on dimple dimensions were observed. These results enable industrial producers to improve the quality of their products by finding optimised textures and controlling nominal surface texture tolerances prior to the rubber components production.
Clinical Performance of CAD/CAM All-Ceramic Tooth-Supported Fixed Dental Prostheses: A Systematic Review and Meta-Analysis
Authors: Saravi Babak , Vollmer Andreas , Hartmann Maja , Lang Gernot , Kohal Ralf-Joachim , Boeker Martin , Patzelt Sebastian B. M.
Published: May 2021

Although CAD/CAM ceramics present a promising alternative to metal-ceramic fixed dental prostheses, little is known about their mid- and long-term clinical performance. This systematic review aims to estimate the survival and success rates and describes the underlying complication characteristics for CAD/CAM tooth-supported zirconia- and lithium disilicate-based fixed dental prostheses (FDPs). We systematically searched MEDLINE and Web of Science to find relevant prospective studies with a follow-up of at least one year. We estimated pooled 1-, 5-, and 10-year survival and success rates by combining the collected data in a Poisson regression model. Descriptive statistics were conducted to evaluate the distribution of failures and complications in the included studies. Risk of bias for the included studies was assessed with an adapted checklist for single-arm trials. Pooled estimated 1-, 5-, and 10-year survival rates ranged from 93.80% to 94.66%, 89.67% to 91.1%, and 79.33% to 82.20%, respectively. The corresponding success rates excluding failures, but including any other types of intervention were 94.53% to 96.77%, 90.89% to 94.62%, and 81.78% to 89.25%. Secondary caries was the most frequent cause of failure, followed by chipping of the veneering. The most common cause of complication excluding failures but requiring intervention was chipping of the veneering. Risk of bias was generally acceptable for the included studies, with seven studies associated with low risk of bias, eight studies with a moderate risk of bias, and three studies with serious risk of bias. The current meta-analysis on CAD/CAM-supported FDPs revealed satisfying survival and success rates for up to 10 years of exposure. More prospective studies focusing on long-term performance are needed to strengthen the evidence currently available in the literature.
Impact of Sulphate Ions Content on Performance of Maleic and Acrylic Superplasticizers in Cement Paste
Authors: Janowska-Renkas Elżbieta
Published: May 2021

The paper presents test results of the impact of sulphate ions from calcium sulphates: Hemihydrate, dihydrate and anhydrite, on rheological properties and hydration heat of cement pastes with, and without, superplasticizers, derivatives of maleic (SP-2) and acrylic (SP-1) acids. It is demonstrated that cement pastes fluidity depends on superplasticizer chemical structure, and its effect is expressed by a hydrophilic coefficient. As maleic superplasticizers have flexible comb-like structure composed of a shorter backbone chain containing COO− carboxylate groups and very long side chains, cement pastes showed higher fluidity than with acrylic superplasticizer with ladder-like structure, longer backbone chains with shorter side chains. SP-1 showing lower hydrophilicity coefficient and fewer COO− groups was found to be less sensitive to increased sulphate ion content in pastes. However, with SP-2 with higher hydrophilicity, a gradual fluidity loss (increased paste viscosity) was observed. Plastic viscosity was approximately at the same level in SP-1-containing pastes. Tests showed that sulphates definitely changed polycarboxylate superplasticizers performance. A high concentration of sulphate ions reduced maleic superplasticizer efficiency. Under these conditions, SP-1 is more effective and therefore more suitable for fluidity of pastes containing higher SO42− ions content. Thus, sulphate ions concentration in the paste should be considered when selecting superplasticizer.
Effect of Heat Treatment on the Microstructure and Performance of Cu Nanofoams Processed by Dealloying
Authors: Gubicza Jenő , Jenei Péter , Han Gigap , Hung Pham-Tran , Song Youngseok , Park Dahye , Szabó Ábel , Kádár Csilla , Kim Jae-Hun , Choe Heeman
Published: May 2021

Cu nanofoams are promising materials for a variety of applications, including anodes in high-performance lithium-ion batteries. The high specific surface area of these materials supports a high capacity and porous structure that helps accommodate volume expansion which occurs as batteries are charged. One of the most efficient methods to produce Cu nanofoams is the dealloying of Cu alloy precursors. This process often yields nanofoams that have low strength, thus requiring additional heat treatment to improve the mechanical properties of Cu foams. This paper provides the effects of heat treatment on the microstructures, mechanical properties, and electrochemical performance of Cu nanofoams. Annealing was conducted under both inert and oxidizing atmospheres. These studies ultimately reveal the underlying mechanisms of ligament coarsening during heat treatment.
Fractional Line Integral
Authors: Bengochea Gabriel , Ortigueira Manuel
Published: May 2021

This paper proposed a definition of the fractional line integral, generalising the concept of the fractional definite integral. The proposal replicated the properties of the classic definite integral, namely the fundamental theorem of integral calculus. It was based on the concept of the fractional anti-derivative used to generalise the Barrow formula. To define the fractional line integral, the Grünwald–Letnikov and Liouville directional derivatives were introduced and their properties described. The integral was defined for a piecewise linear path first and, from it, for any regular curve.