bluuebunny/crossref_metadata_2025 huggingface dataset:
Anlambilimsel Boyutta Derin ve Yüzey Yapı: Mercân Fersâd’ın Evimiz Adlı Şarkı Sözlerinin Göstergeler Açısından İncelenmesi
Authors: Gedik Ümit
Published: June 2024

Anlamsal açıdan çok boyutluluğu barındıran müzik eserlerinde yer alan şarkı sözlerinde her bir sözcük göstergesinin sembolik karakter taşıdığı gözlemlenmektedir. Şarkı sözlerindeki sembolik karakterlere sahip bu sözcük göstergelerinin somut anlamları ile kullanılma zorunluluğu bulunmamaktadır. Bu nedenle daha ziyade duygusal göstergelerin hâkim olduğu şarkı sözlerinde sanatçılar tarafından tercih edilen göstergelerin somut anlamları dışında sahip olduğu derin anlamların incelenmesi ve yorumlanması önemlidir. Bu önemle belirtilen derin anlamlar, dilbilimin alt dallarından, sözcüklerin farklı anlam boyutlarını ve bu anlamların türlerini belirlemeyi amaçlayan anlambilim ve edimbilim araştırmaları doğrultusunda analiz edilebilmektedir. Bu çalışmada, Mercân Fersâd’ın ilk albümüهای آبی گل /Mavi Çiçekler’de yer alan خانه ما/Evimiz adlı şarkısında yer alan şarkı sözlerinde tercih ettiği göstergeler ve göstergelerin sahip olduğu tasarım, imgelerin anlambilim/edimbilimsel açıdan yüzey ve derin yapı boyutlarıyla analiz edilmesi amaçlanmaktadır. Çalışmada, “Sanatçının, Evimiz adlı şarkı sözlerinde üretmiş olduğu mesajları hangi amaca yöneliktir? Fersâd bu amaç doğrultusunda şarkı sözlerinde hangi göstergeleri tercih etmiştir?” araştırma sorularına yanıt aranacak ve şarkıda dile getirilmiş göstergeler üzerinden anlambilimsel açıdan derin yapıda hangi mesajların verilmek istendiği ortaya konulmaya çalışılacaktır.
MÉTODOS DE INVESTIGACIÓN PARA LA INNOVACIÓN A TRAVÉS DEL DISEÑO. UN ENFOQUE CONTEMPORÁNEO
Authors: Bedón Vaca Concepción del Carmen , Acurio-Maldonado Daniel Marcelo , Quispe Morales Michele Paulina
Published: June 2024

El presente trabajo pretende explorar la importancia de los métodos de investigación para procesos de innovación desde el diseño. Esto se debe a que el diseño es un factor clave para el crecimiento y desarrollo de las organizaciones en un entorno empresarial cada vez más dinámico; por ello, resulta importante comprender y aplicar métodos de investigación efectivos, que fomenten la competitividad. Con respecto al objetivo de estudio, se examina la manera en la que los métodos de investigación influyen en la implementación de proyectos innovadores. Se analizan diferentes procesos utilizados en la investigación, para lo que se usa, como metodología, una revisión de la literatura académica y una investigación relacionada con procesos de innovación. Se examinan estudios empíricos y revisiones sistemáticas, para obtener una visión completa de los diferentes métodos utilizados. El principal resultado de este estudio es proporcionar una síntesis de las metodologías más efectivas y ofrecer recomendaciones prácticas para investigadores y profesionales interesados en impulsar la innovación desde el diseño. Se espera que este artículo contribuya al conocimiento de la investigación y promueva la adopción de enfoques eficientes para fomentar la innovación en diversos sectores.Palabras clave: métodos de investigación, innovación, metodología, generación de ideas, proyectos de innovación, diseño.AbstractThis paper explores the importance of research methods for innovation processes from design. As the latter is a key factor for the growth and development of organizations in an increasingly dynamic business environment, it is important to understand and apply effective research methods that promote competitiveness. Regarding the study's objective, the way research methods influence the implementation of innovative projects is examined. Different processes used in research are analyzed, and their effectiveness is evaluated in terms of identifying opportunities, generating ideas, selecting projects, and managing innovation and design. The methodology is based on a review of academic literature and research related to innovation processes. Empirical studies and systematic reviews are examined to overview the different methods used comprehensively. The main result of this study is to provide a synthesis of the most effective methodologies and offer practical recommendations for researchers and professionals interested in driving innovation from design. This article is expected to contribute to research knowledge
Keywords: research methods, innovation, methodology, generation of ideas, innovation projects, design.
EL DISEÑO DE UNA EXCLUSIÓN POR EFECTO
Authors: Caballero Quiroz Aarón J.
Published: June 2024

La asociación que frecuentemente se hace entre método y diseño, o incluso la equivalencia entre uno y otro, revela las representaciones que epistemológicamente hace de sí mismo, de sus prácticas y, por lo tanto, del mundo que genera. La naturaleza cientificista del método al que se acude en el diseño es, por definición, discriminatoria, debido a que, como todo acto objetivo basado en la razón, debe establecer cercos conceptuales que delimiten el problema que se pretende atender. Una práctica como la anterior, delante la autodeterminación en que las personas quedan constituidas, representa severos problemas de exclusión en ellas, por la determinación que la razón hace de estas. Así, puede operar metódicamente, al menos en lo que al diseñose refiere. El presente trabajo expone detenidamente las afinaciones que se hacen a este respecto en términos siempre epistemológicos y con un entendimiento del diseño, ante todo, como un acto de “trabajo” bajo el que es posible conocer y, en consecuencia, autodeterminarse.Palabras clave: diseño, método, exclusión, epistemología, autodeterminación.
AbstractThe association often drawn between method and design, or even the perceived equivalence between the two, reveals the epistemological representations it creates of itself, its practices, and, thus, of the world it generates. The scientific nature of the method used in design is discriminatory because, like any objective act grounded in reason, it must establish conceptual boundaries that define the problem to be addressed. When faced with the self-determination inherent in human beings, this practice poses significant exclusionary issues due to the dictates of reason that it must adhere to function methodically, particularly in the realm of design. This work presents in detail the refinements made in this regard, always in epistemological terms, and underscores the understanding of design as an act of 'work' under which knowledge and, consequently, self-determination can be attained.
Keywords: design, method, exclusion, epistemology, self-determination.
Proyecto de conservación de trece thangkas tibetanos de la Fundación Pública Andaluza Rodríguez-Acosta
Authors: Ángel Gómez Carmen , Fernández González Lourdes , Gómez Morón Auxiliadora , Morales Muñoz Maira
Published: June 2024

La intervención de estos trece thangkas, pertenecientes a la Fundación Pública Andaluza Rodríguez-Acosta, ha supuesto para los profesionales del Instituto Andaluz del Patrimonio Histórico un reto y una oportunidad para ampliar el conocimiento sobre las técnicas pictóricas y textiles empleadas en unas obras procedentes del Tibet. La presencia de tejidos que ya no se realizan, las diferencias pictóricas con las obras occidentales, los hacen ser ejemplares únicos de estudio, que se ha abordado con técnicas no invasivas portátiles de análisis que se ha llevado a cabo para la caracterización de los materiales sin necesidad de tomar muestras.
Investigación y ciencias experimentales del patrimonio: buenas prácticas en el IAPH
Authors: Sameño Puerto Marta , García Lourdes Martín , Sáenz de Lacuesta Sáez de Ocáriz Héctor , Bernáldez Sánchez Eloísa
Published: June 2024

En este artículo, se examinarán en detalle las prácticas y estrategias adoptadas por el Instituto Andaluz del Patrimonio Histórico (IAPH), como entidad científica, para promover la excelencia en la investigación del patrimonio y el papel que desempeñan las ciencias experimentales en el avance del conocimiento patrimonial. El artículo aborda la evolución histórica de la relación entre la ciencia y el patrimonio cultural, destacando la importancia de la interdisciplinariedad en la investigación para abordar los desafíos que presenta la conservación del patrimonio. Se incide en la creación de laboratorios especializados, donde un grupo de investigadores llevan más de treinta años estudiando el patrimonio cultural de Andalucía para un mejor conocimiento de la composición de todo tipo de materiales que forman parte de los bienes culturales y de sus procesos de alteración, así como la evaluación de tratamientos que ayuden a su conservación. Con el desarrollo de técnicas no invasivas de análisis y la integración de la ciencia experimental en la preservación del patrimonio como elementos clave en este campo, se hace hincapié en la importancia de la sostenibilidad y la integración de la innovación y la tradición en la conservación del patrimonio histórico. El artículo también menciona los grupos de investigación, los proyectos I+D y la inversión del IAPH en equipos científicos e infraestructuras tecnológicas para fortalecer la capacidad de investigación y conservación del patrimonio cultural. Por último, la divulgación de las ciencias experimentales en el ámbito del patrimonio se destaca como una herramienta fundamental para sensibilizar a la sociedad sobre la importancia de la conservación, buscando no solo preservar el patrimonio para las generaciones futuras, sino también promover su conocimiento y su valor.
Programa I+ D y de conservación de los efebos de Pedro Abad. Modelo de gestión para la intervención
Authors: Gómez Villa José Luis , Ojeda Calvo Reyes
Published: June 2024

Las necesidades de preservar desde el rigor científico los objetos que nos caracterizan culturalmente como sociedad y que elevamos a las máximas figuras de protección patrimonial vienen siendo uno de los retos del IAPH desde su creación. Estos procesos requieren de una constante y continua renovación científica y técnica, pero también de una capacidad de gestión, interrelación y aportación de recursos que, más allá del rango de las profesiones y especialistas en el ámbito de la cultura, demanda estrategias con las que simplificar procesos y aunar esfuerzos. Este es el caso del Programa de I+D y de conservación de los Efebos de Pedro Abad. Un proyecto que debía responder con excelencia al carácter único de unas piezas singulares que transcienden a nuestra comunidad para convertirse en referencia internacional.
Entrepreneurship Teaching : Design and Activities of 21st Century Learning for Economic Class under Mini-Project (Participatory-Phenomenological Design)
Authors: Fahrurrozi Muh
Published: June 2024

This study aims to explain the process of teaching entrepreneurship education courses in economics classes under a detailed mini-project. This research used Participatory action research (PAR) under qualitative approach. The sample size was only taken purposefully from one semester's three economics classes. This research used observation sheets in the form of learning activities and interviews.  All data collected were analyzed descriptively with the step of reducing, displaying and drawing conclusion. The research results showed the effectiveness of a bidirectional teaching model in entrepreneurship education. This model, which combines theoretical instruction with practical application, significantly enhances student engagement and learning outcomes. The bidirectional approach allows students to relate theoretical concepts to real-world examples, fostering a deeper understanding and active participation. An essential element of this teaching model is the inclusion of mini-projects. These projects enable students to apply theoretical knowledge in practical scenarios, such as developing business plans and analyzing recruitment strategies. The hands-on experience gained through mini-projects not only solidifies students' grasp of the material but also hones their problem-solving and critical-thinking skills. The study's findings underscore the superiority of the bidirectional teaching model over traditional methods. The experimental class, which utilized this approach, showed a 93% success rate in meeting various evaluation criteria, compared to just 30% in the control class. This stark contrast highlights the importance of integrating mini-projects and practical activities into the curriculum to enhance educational outcomes. The bidirectional teaching model, complemented by mini-projects, proves to be a highly effective approach in entrepreneurship education. It not only engages students more deeply but also equips them with practical skills and knowledge that are essential for their future careers.
Understanding Vocational School Students’ Work Readiness : The Synergistic Role of Fieldwork Practice and Self-Efficacy
Authors: Yuliyanto Riyan , Susilaningsih Susilaningsih , Indriayu Mintasih
Published: June 2024

This study aims to analyze the synergy between fieldwork practices and self-efficacy in improving the work readiness of vocational school students. This research used a survey method with a quantitative approach. This study involved 1289 populations and 306 samples from three state vocational schools in Surakarta, selected using a proportionate random sampling method. The instruments used in this study consisted of Fieldwork Practice (FWP), Self-efficacy (SE), and Work Readiness (WR) questionnaires. The data obtained were analyzed by statistical testing of descriptive analysis, correlation, and mediation using path analysis. The results of this study revealed that fieldwork practices and self-efficacy contribute significantly to students’ work readiness. The analysis shows that self-efficacy serves as an important mediator in the relationship between fieldwork practices and work readiness, reinforcing the role of practice-based education in preparing students for the world of work. In this study, it was found that the development of education and training programs that focus on improving students’ self-efficacy through fieldwork practices can be a major key in the school-to-work transition.
Developing A Differentiated Learning Model Based on Artificial Intelligence : Implementation in The Mathematics Classroom
Authors: Sunarto M. J. Dewiyani , Hariadi Bambang , Lemantara Julianto
Published: June 2024

This study aims to produce a model that can meet the needs of Differentiated Learning based on AI. This method used research and development (R&D) with the Generic Desain Research Model (GDRM). The subject of this study was mathematics education experts and mathematics learning model experts. Instruments used in this study include quality validity assessment and feasibility assessment analysis. The data analysis techniques used were quantitative methods with learning model validity assessment criteria that use A Linkert Scale of 1-4. The test results of the AI-based Mathematics learning model show that (1) the Content Validity test resulted in an average of 3.47 which means the model is very valid, and (2) The average feasibility test is 3.43, which means it is also very valid. The results showed that the AI-based Mathematics learning model can be implemented in differentiated mathematics classes and train students to learn independently, think critically, provide solutions to problems, and have the courage to convey solutions.
Development of a Higher-Order Thinking Skills (HOTS) Test Instrument on Electrochemical Material for High School Students
Authors: Nugrahnastiti Nuzulul , Kamaludin Agus
Published: June 2024

This research aims to develop a HOTS test instrument on electrochemistry topics that meets valid and reliable criteria. Apart from that, the level of difficulty, distinguishing power, ability level, and detection of respondent bias were also analyzed. This research is development research using the Tessmer model, which includes preliminary and formative evaluation stages. The HOTS questions consist of 15 multiple-choice questions. The validation data from experts is measured using a Likert scale. Coefficient Aikens’ V is then used to measure the validation data from reviewers. Experts and reviewer assessments indicated that the HOTS question instrument developed was very good. Analysis of questions using the Rasch model in a field testing of 33 high school students using a purposive sampling technique resulted in 14 valid questions and 1 invalid question with a reliability value of 0,69. There were 3 questions in the easy category, 7 in the moderate category, 1 in the hard category, and 4 in the extremely hard category. The analysis of the distinguishing power of the questions obtained 9 questions with very good criteria, 3 questions with good criteria, and 2 with fair criteria. The results of the validity of the students` responses showed that there were 4 invalid responses. The ability level shows that 5 students have high ability, 7 have moderate ability, and 21 have low ability. In conclusion, the developed HOTS test instrument for electrochemical material has a good quality and is suitable for evaluation.
Public Libraries as Incubators for Social Inclusion and Entrepreneurship for Achieving Sustainable Development Goals (SDGs) : A Progressive Transformation
Authors: Haryanto Haryanto , Laugu Nurdin , Zulaikha Sri Rohyati
Published: June 2024

This research aims to explore the role of public libraries as incubators of social inclusion and entrepreneurship in supporting the achievement of the Sustainable Development Goals (SDGs). This study employed a qualitative approach with a case study method, focusing on public libraries in Karanganyar Regency, Central Java, Indonesia, that have implemented social inclusion and entrepreneurship programs. The data were collected through in-depth interviews with librarians, program participants, and relevant stakeholders, as well as direct observation and program document analysis. The data analysis followed the framework proposed by Miles and Huberman, which involves a systematic approach to qualitative data analysis, including data reduction, data display, and conclusion drawing/verification. The results showed that libraries functioning as entrepreneurship incubators can effectively provide platforms for business training, technology access, and network development. These programs enhanced individual's skills and knowledge and drived local economic growth by creating new businesses and increasing employment opportunities. Additionally, library social inclusion initiatives boost community participation and engagement, strengthen social cohesion, and reduce inequality. The research concludes that public libraries in Karanganyar Regency have significant potential as agents of social change by integrating social inclusion and entrepreneurship programs. To optimize this role, adequate policy support, enhanced librarian competencies, and collaboration with various stakeholders are necessary. Thus, libraries can significantly contribute to achieving the SDGs, particularly in quality education, decent work and economic growth, reduced inequalities, and partnerships for the goals.
Development of An Interactive E-Module Based on Blended Learning to Enhance Student Independence and Learning Outcomes
Authors: Andriani Novia , Dwiningsih Kusumawati
Published: June 2024

This study aims to analyze the feasibility and effectiveness of interactive e-modules based on blended learning to increase independence and learning outcomes on acid-base materials. This research is development research using the Borg & Gall model which consists of ; 1) Research and Information collection, 2) Product planning, 3) Product development, 4) Product validation, 5) Product revision, 6) Product trial. The subjects of this study were chemistry lecturers and chemistry teachers as well as students of SMA Negeri 1 Krembung Sidoarjo who took chemistry specialization. The instruments used were review and validation sheets, learning independence questionnaires, response questionnaires, and learning outcomes test sheets. Data analysis using the N-gain to determine the increase in student independence and learning outcomes. The validation results are processed using the mode of each statement, the results of content validity get mode 5 with very valid criteria, and construct validity get mode 4 with valid criteria. The results of this study indicate that learning chemistry using interactive e-modules based on blended learning can increase learning independence with an n-gain value at 3 meetings of 0.49 in the medium category and learning outcomes with an average n-gain value of 0.69 in the moderate category. Therefore, the interactive e-module based on blended learning developed is feasible and effective to be used to improve students' independence and learning outcomes on acid-base materials.
Burden of mortality from hepatocellular carcinoma and biliary tract cancers by race and ethnicity and sex in US, 2018–2023
Authors: Kim Donghee , Manikat Richie , Wijarnpreecha Karn , Cholankeril George , Ahmed Aijaz
Published: June 2024

Backgrounds/Aims: The trends in mortality of hepatocellular carcinoma (HCC) and biliary tract cancers stratified by sex and race/ethnicity in the US continue to evolve. We estimated the sex- and race/ethnicity-based trends in HCC and biliary tract cancers-related mortality in US adults with a focus on disease burden.Methods: We performed a population-based analysis using the US national mortality records from 2018 to 2023. We identified HCC and biliary tract cancer using appropriate ICD-10 codes. Temporal trends in mortality were calculated by joinpoint analysis with annual percentage change (APC).Results: Annual age-standardized mortality from HCC decreased steadily with an APC of –1.4% (95% confidence interval [CI]: –2.0% to –0.7%). While there was a linear increase in intrahepatic cholangiocarcinoma-related mortality (APC: 3.1%, 95% CI: 1.2–4.9%) and ampulla of Vater cancer-related mortality (APC: 4.1%, 95% CI: 0.5–7.9%), gallbladder cancer-related mortality decreased (APC: –1.9%, 95% CI: –3.8% to –0.0%). Decreasing trends in mortality from HCC were noted in males, not females. HCC-related mortality decreased more steeply in racial and ethnic minority individuals compared with non-Hispanic White individuals. Racial and ethnic differences in trends in mortality for biliary tract cancers depended on the malignancy’s anatomical site.Conclusions: While the annual mortality for HCC and gallbladder cancer demonstrated declining trends, ICC- and AVC-related mortality continued to increase from 2018 to 2023. Although racial and ethnic minority individuals in the US experienced disproportionately higher HCC and biliary tract cancer, recent declines in HCC may be primarily due to declines among racial and ethnic minority individuals and males.
EVALUASI KEBIJAKAN PEMENUHAN HAK PEKERJAAN SEKTOR FORMAL BAGI PENYANDANG DISABILITAS DI LINGKUNGAN PEMERINTAH KOTA BANDUNG
Authors: Syahfitri Cut Novi , Ilham Muh. , Mulyati Deti , Tjenreng M. Zubakhrum B.
Published: June 2024

Penelitian ini difokuskan pada evaluasi kebijakan penyelenggaraan penyandang disabilitas dalam pemenuhan hak pekerjaan sektor formal di Kota Bandung berdasarkan pada teori evaluasi William N. Dunn yang mencakup 6 (enam) kriteria, yaitu efektivitas, efisiensi, kecukupan, pemerataan, responsivitas, ketepatan. Penelitian ini bertujuan untuk menganalisis evaluasi kebijakan penyelenggaraan perlindungan panyandang disabilitas dalam pemenuhan hak pekerjaan sektor formal di Kota Bandung dan memberikan rekomendasi kebijakan dan model implementasi kebijakan terkait dengan penyelenggaraan perlindungan penyandang disabilitas dalam pemenuhan hak pekerjaan sektor formal di Kota Bandung.
Penelitian menggunakan ini menggunakan metode kualitatif eksploratif dengan pendekatan induktif. Wawancara, teknik dokumentasi, observasi, dan triangulasi merupakan teknik pengumpulan data yang digunakan dalam penelitian ini. Hasil penelitian ini dapat disimpulkan bahwa evaluasi kebijakan penyelenggaraan penyandang disabilitas dalam pemenuhan hak pekerjaan sektor formal di Kota Bandung belum optimal hal ini dapat dilihat kebijakan yang sudah berjalan sejak diterbitkannya sampai dengan penelitian ini dibuat belum memenuhi keenam kriteria, yaitu efektivitas, efisiensi, kecukupan, kesamaan, reponsivitas, dan ketepatan.
Berdasarkan analisis terhadap hasil tersebut, penelitian ini mengusulkan rekomendasi kebijakan yaitu meningkatkan kolaborasi dan integritas pemerintah, penguatan peran pemerintah, mempercepat revisi Peraturan Daerah Provinsi Jawa Barat terkat penyandang disabililitas yang nantinya akan dijabarkan melalui Peraturan Wali Kota Bandung, sinkronisasi data dan informasi yang tepat dan akurat, dan penyusunan kebijakan pendukung Peraturan Daerah Pemerintah Provinsi Jawa Barat tentang Penyandang Disabilitas. Adapun model implementasi kebijakan yaitu kepemimpinan, komunikasi, substansi kebijakan, pengawasan, dan sumber daya.
The Presentation of the Risks That Oil Pollution Poses to Aquaculture
Authors: Aşan Cihat
Published: June 2024

Aquaculture has emerged as the most rapidly growing technology for food production on a global scale. The current growth trajectory of aquaculture production surpasses that of all other meat production types and is anticipated to persist in its ascent with the continuous expansion of the agriscience industry. The contribution of aquaculture to food security varies based on species and country, either directly through domestic consumption or indirectly through the stimulation of economic growth via exports. In Türkiye, the share of aquaculture in overall production has increased over the years and has become a significant contributor to the country’s economic development through exports. Fish farms concentrated in the Eastern Black Sea and Southern Aegean regions also draw attention due to their proximity to sea areas with intense maritime activities. Potential marine pollution in these regions poses a serious risk to these economically important resources, making it crucial to predict the extent of this risk in advance and take preventive measures. This study aims to simulate, through a scenario, how a possible oil spill in the Southern Aegean region would spread in the sea, how quickly it would reach the fish farms in this region, and how long it would take to impact the fish population to a certain extent. The values derived from this simulation will shed light on intervention plans to be implemented by both local and central authorities, serving as exemplary models for formulating similar plans for all aquaculture resources.
Penyelesaian Kumulasi Perkara Isbat Nikah dan Cerai Talak dalam Putusan Nomor 0307/Pdt.G/2018/PA.Sby
Authors: Arif Alfiyah Faizatul
Published: June 2024

In the practice of proceedings in the Religious Courts, judges generally immediately apply Article 7 Paragraphs (2) and (3) of the Compilation of Islamic Law (KHI) without testing the strength of its enforceability before the law. Thus, even though these provisions are difficult to understand, the majority of Religious Court judges, with their interpretation, understand the provisions in Paragraph (3) letter a, as if it is mandatory to accept the application for itsbat marriage if it is submitted in combination with the application for talak divorce, even though the marriage was carried out after its entry into force. Law no. 1 of 1974. The consideration of the marriage isbat law itself is still a matter of polemic. Bearing in mind that the position of KHI which is the basic reference for legal considerations by judges is not included in the statutory hierarchy. This happened in the divorce divorce case Decision Number 0307/Pdt.G/2018/PA.Sbg. Several issues can be raised that become a polemic regarding the marriage isbat law, namely how the essence of the marriage isbat law is related to the essence of the marriage isbat law in the marriage legal system in Indonesia. Then what is the legal position of marriage isbat in the Religious Courts which was implemented after Law Number 1 of 1974. The provisions for marriage isbat in Indonesia only came into existence after the birth of Law Number 1 of 1974 concerning Marriage. In addition, the law has given legitimacy to judges to further explore the values ??and sense of justice in society.
An Evaluation of The Relationship Between Falls, Osteoporosis And The Parkinson’s Disease Rating Scale In Patients With Parkinson’s Disease
Authors: Keles Onal Asli , Özdolap Çoban Şenay , Sarıkaya Selda , Aciman Demirel Esra , Özdemir Sibel
Published: June 2024

Objective: The purpose of this study is to examine conditions such as balance disorder, risk of falling, fear of falling, vitamin D deficiency and osteoporosis in people with Parkinson's Disease (PD), and their association with the Movement Disorders Society Unified Parkinson’s Disease Rating Scale (MDS-UPDRS) sub-components, which are used to follow-up these patients.
Methods: The study comprised 38 patients who were followed up for idiopathic PD. All the patients’ demographic data, falls efficacy scale, number of falls within the last year, history of fractures, Berg Balance Scale, the MDS-UPDRS sub-components, vitamin D levels, and bone mineral densitometry values were recorded.
Results: There was a positive correlation between the MDS-UPDRS Part I and the number of falls and the history of fractures, and a positive correlation with the Hoehn and Yahr scale, and the MDS-UPDRS Part II, III and total and the Berg Balance Scale, the Falls Efficacy Scale, and the number of falls. Our study found that the bone mineral densitometry values for the femoral neck were lower in women than in men, and there was a positive correlation between the bone mineral densitometry values for the femoral neck and the body mass index. A positive correlation was established between levodopa use and the falls efficacy scale.
Conclusion: Falls, imbalance, osteoporosis are life-threatening conditions in patients with PD. This study established that the MDS-UPDRS, used to follow-up patients, was associated with these conditions. It is believed that this assessment method may also give an idea about these conditions in PD patients who are followed up using this scale.
The Used Masks of The Food Industry Employees During the Covid-19 Pandemic: Did the Mask Promote Other Diseases While Protecting from the Coronavirus? A Survey Study Supported by Microbiological Data
Authors: Dinçoğlu Ahmet Hulusi , Özgür Mustafa , Özgür Elif Büşra , Çalışkan Zühal , Rugji Jerina
Published: June 2024

Objective: Use of disposable face masks helps prevent infection by airborne pathogens. The effectiveness of such masks in excluding diseases and contaminants depends on many factors. As a result of misuse, mask loses its protective role and becomes a source of microbial contamination. It is aimed to investigate the attitudes of food workers towards use of masks in proportion to the bacterial load and microorganism species in the masks they wear.
Methods: Total aerobic mesophilic, Yeast-mould and Coliform counts were determined as log colony forming units per mL. Phenotypically different colonies grown on three different agar plates were purified and fresh cultures were classified using matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
Results:In this study, bacterial contamination was found at different levels on all tested (103) disposable face masks. The screened bacterial pathogens by MALDI-TOF MS Bacillus cereus was detected at the highest level 17.86%, Enterobacter cloacae, Klebsiella oxytoca, Kurthia gibsonii and Pseudomonas aeruginosa with rates 14.29%, 10.72%, 7.14%, and 7.14%, respectively. Long-term working, inappropriate mask usage, poor hygiene attitudes of employees, and the fact that staff wore the mask out of need rather than for its protective advantage all signal that the investigated masks had low microbiological quality.
Conclusion:The findings show that because most masks used by food industry employees to protect themselves from COVID-19 and avoid infecting others contain bacteria of intestinal origin, serious health problems may occur in both employees who use contaminated masks and consumers who consume food contaminated by mask contamination.
Mechanical loading and orthobiologic therapies in the treatment of post-traumatic osteoarthritis (PTOA): a comprehensive review
Authors: Gardashli Mahammad , Baron Max , Huang Charles , Kaplan Lee D. , Meng Zhipeng , Kouroupis Dimitrios , Best Thomas M.
Published: June 2024

The importance of mechanical loading and its relationship to orthobiologic therapies in the treatment of post-traumatic osteoarthritis (PTOA) is beginning to receive attention. This review explores the current efficacy of orthobiologic interventions, notably platelet-rich plasma (PRP), bone marrow aspirate (BMA), and mesenchymal stem/stromal cells (MSCs), in combating PTOA drawing from a comprehensive review of both preclinical animal models and human clinical studies. This review suggests why mechanical joint loading, such as running, might improve outcomes in PTOA management in conjunction with orthiobiologic administration. Accumulating evidence underscores the influence of mechanical loading on chondrocyte behavior and its pivotal role in PTOA pathogenesis. Dynamic loading has been identified as a key factor for optimal articular cartilage (AC) health and function, offering the potential to slow down or even reverse PTOA progression. We hypothesize that integrating the activation of mechanotransduction pathways with orthobiologic treatment strategies may hold a key to mitigating or even preventing PTOA development. Specific loading patterns incorporating exercise and physical activity for optimal joint health remain to be defined, particularly in the clinical setting following joint trauma.
RNAseq of INOCA patients identifies innate, invariant, and acquired immune changes: potential autoimmune microvascular dysfunction
Authors: Jaatinen Kevin , Shah Palak , Mazhari Ramesh , Hayden Zane , Wargowsky Richard , Jepson Tisha , Toma Ian , Perkins John , McCaffrey Timothy A.
Published: June 2024

Background

Ischemia with non-obstructive coronary arteries (INOCA) is a major clinical entity that involves potentially 20%–30% of patients with chest pain. INOCA is typically attributed either to coronary microvascular disease and/or vasospasm, but is likely distinct from classical coronary artery disease (CAD).

Objectives

To gain insights into the etiology of INOCA and CAD, RNA sequencing of whole blood from patients undergoing both stress testing and elective invasive coronary angiography (ICA) was conducted.

Methods

Stress testing and ICA of 177 patients identified 40 patients (23%) with INOCA compared to 39 controls (stress-, ICA-). ICA+ patients divided into 38 stress- and 60 stress+. RNAseq was performed by Illumina with ribosomal RNA depletion. Transcriptome changes were analyzed by DeSeq2 and curated by manual and automated methods.

Results

Differentially expressed genes for INOCA were associated with elevated levels of transcripts related to mucosal-associated invariant T (MAIT) cells, plasmacytoid dendritic cells (pcDC), and memory B cells, and were associated with autoimmune diseases such as rheumatoid arthritis. Decreased transcripts were associated with neutrophils, but neutrophil transcripts, per se, were not less abundant in INOCA. CAD transcripts were more related to T cell functions.

Conclusions

Elevated transcripts related to pcDC, MAIT, and memory B cells suggest an autoimmune component to INOCA. Reduced neutrophil transcripts are likely attributed to chronic activation leading to increased translation and degradation. Thus, INOCA could result from stimulation of B cell, pcDC, invariant T cell, and neutrophil activation that compromises cardiac microvascular function.
Association between life’s essential 8 and testosterone deficiency in US men: findings from national health and nutrition examination survey (NHANES) 2011–2016
Authors: Mei Yangyang , Ji Nuo , Zhang Bo , Xia Wei , Feng Xingliang , Xu Renfang , Xue Dong
Published: June 2024

Background

Testosterone deficiency (TD) is closely associated with cardiovascular diseases (CVD). We intended to explore the association of Life’s Essential 8 (LE8), the recently updated measurement of cardiovascular health, with the prevalence of TD among US male adults.

Methods

The population-based cross-sectional study selected male adults aged 20 years or older from the National Health and Nutrition Examination Survey (NHANES) from 2011 to 2016. According to the American Heart Association definitions, the LE8 score was measured on a scale of 0–100, and divided into health behavior and health factor scores, simultaneously. Furthermore, these scores were categorized into low (0–49), moderate (50–79), and high (80–100) classifications. TD is defined as a total testosterone level below 300ng/dL. Correlations were investigated by weighted multivariable logistic regression, and the robustness of the results were verified by subgroup analysis.

Results

A total of 4971 male adults with an average age of 47.46 ± 0.41 years were eligible for the final analyses, of whom 1372 were determined to have TD. The weighted mean LE8 score of the study population was 68.11 ± 0.41. After fully adjusting potential confounders, higher LE8 scores were significantly associated with low risk of TD (odd ratio [OR] for each 10-point increase, 0.79; 95% CI, 0.71–0.88) in a linear dose-response relationship. Similar patterns were also identified in the association of health factor scores with TD (OR for each 10-point increase, 0.74; 95% CI, 0.66–0.83). These results persisted when LE8 and health factor scores was categorized into low, moderate, and high groups. The inversed association of LE8 classifications and TD remained statistically significant among older, obese, and men without CVD.

Conclusions

LE8 and its health factor subscales scores were negatively associated with the presence of TD in linear fashions. Promoting adherence to optimal cardiovascular health levels may be advantageous to alleviate the burden of TD.
Novel perspectives on autophagy-oxidative stress-inflammation axis in the orchestration of adipogenesis
Authors: Hong Chun , Li Xinming , Zhang Kunli , Huang Qiuyan , Li Baohong , Xin Haiyun , Hu Bin , Meng Fanming , Zhu Xiangxing , Tang Dongsheng , Hu Chuanhuo , Tao Chenyu , Li Jianhao , Cao Yang , Wang Hai , Deng Bo , Wang Sutian
Published: June 2024

Adipose tissue, an indispensable organ, fulfils the pivotal role of energy storage and metabolism and is instrumental in maintaining the dynamic equilibrium of energy and health of the organism. Adipocyte hypertrophy and adipocyte hyperplasia (adipogenesis) are the two primary mechanisms of fat deposition. Mature adipocytes are obtained by differentiating mesenchymal stem cells into preadipocytes and redifferentiation. However, the mechanisms orchestrating adipogenesis remain unclear. Autophagy, an alternative cell death pathway that sustains intracellular energy homeostasis through the degradation of cellular components, is implicated in regulating adipogenesis. Furthermore, adipose tissue functions as an endocrine organ, producing various cytokines, and certain inflammatory factors, in turn, modulate autophagy and adipogenesis. Additionally, autophagy influences intracellular redox homeostasis by regulating reactive oxygen species, which play pivotal roles in adipogenesis. There is a growing interest in exploring the involvement of autophagy, inflammation, and oxidative stress in adipogenesis. The present manuscript reviews the impact of autophagy, oxidative stress, and inflammation on the regulation of adipogenesis and, for the first time, discusses their interactions during adipogenesis. An integrated analysis of the role of autophagy, inflammation and oxidative stress will contribute to elucidating the mechanisms of adipogenesis and expediting the exploration of molecular targets for treating obesity-related metabolic disorders.
Functional composition of initial soil fungi explains the difference in mass loss of Phragmites australis litter in different habitat conditions across multiple coastal wetlands
Authors: Ping Yunmei , Pan Xu , Cui Lijuan , Li Wei , Hu Yukun , Cornelissen Johannes H. C.
Published: June 2024

The differences in mass loss of leaf litter are primarily thought to be driven by microbial activity, especially by fungi. However, the existence of such differences across large spatial scales has not been well explored in field studies and the underlying mechanisms of difference are still unclear, especially for the role of different fungal guilds in driving different mass losses. We conducted a 1-year decomposition study within each of four coastal wetlands in China to test the difference in mass loss across a large spatial scale (ranging from 26° N to 41° N in latitude). In each wetland, six sites including three composed of P. australis and three composed of another dominant plant species typically in coastal ecosystems were selected. We used P. australis leaf litter as the standard decomposition material, placing it into litter bags with mesh sizes 1 mm and 4 mm, respectively. Final litter mass loss was examined approximately after 3, 9 and 12 months. The different mass loss was quantified using additional mass loss at P. australis sites compared to that at another species sites. We found that the mass loss of leaf litter of P. australis showed a clear difference across multiple coastal wetlands only at later stages of decomposition, which was independent of mesofauna (mesh size) contribution to decomposition. Furthermore, the observed difference in mass loss was primarily attributed to the dissimilarities in initial soil fungal community, particularly the symbiotrophic fungi, rather than the soil bacterial community. Our results provide empirical evidence of a large-scale difference in mass loss in litter decomposition and have linked the observed difference to different soil fungal guilds. These results indicate that symbiotrophic fungi might play a direct or indirect role in driving difference in mass loss, which contributes to a better understanding and invites in-depth further investigation on the underlying microbe-driven mechanisms of the difference.
Integrated analysis of single-cell sequencing and machine learning identifies a signature based on monocyte/macrophage hub genes to analyze the intracranial aneurysm associated immune microenvironment
Authors: Xu Yifan , Guo Pin , Wang Guipeng , Sun Xiaojuan , Wang Chao , Li Huanting , Cui Zhenwen , Zhang Pining , Feng Yugong
Published: June 2024

Monocytes are pivotal immune cells in eliciting specific immune responses and can exert a significant impact on the progression, prognosis, and immunotherapy of intracranial aneurysms (IAs). The objective of this study was to identify monocyte/macrophage (Mo/MΦ)-associated gene signatures to elucidate their correlation with the pathogenesis and immune microenvironment of IAs, thereby offering potential avenues for targeted therapy against IAs. Single-cell RNA-sequencing (scRNA-seq) data of IAs were acquired from the Gene Expression Synthesis (GEO) database. The significant infiltration of monocyte subsets in the parietal tissue of IAs was identified using single-cell RNA sequencing and high-dimensional weighted gene co-expression network analysis (hdWGCNA). The integration of six machine learning algorithms identified four crucial genes linked to these Mo/MΦ. Subsequently, we developed a multilayer perceptron (MLP) neural model for the diagnosis of IAs (independent external test AUC=1.0, sensitivity =100%, specificity =100%). Furthermore, we employed the CIBERSORT method and MCP counter to establish the correlation between monocyte characteristics and immune cell infiltration as well as patient heterogeneity. Our findings offer valuable insights into the molecular characterization of monocyte infiltration in IAs, which plays a pivotal role in shaping the immune microenvironment of IAs. Recognizing this characterization is crucial for comprehending the limitations associated with targeted therapies for IAs. Ultimately, the results were verified by real-time fluorescence quantitative PCR and Immunohistochemistry.
SPI1-mediated macrophage polarization aggravates age-related macular degeneration
Authors: Qi Siyi , Zhang Yihan , Kong Lingjie , Bi Daode , Kong Hongyu , Zhang Shujie , Zhao Chen
Published: June 2024

Objective

This study revealed a core regulator and common upstream mechanisms for the multifaceted pathological processes of age-related macular degeneration (AMD) and provided proof-of-concept for this new therapeutic target.

Methods

Comprehensive gene expression analysis was performed using RNA sequencing of eye cup from old mice as well as laser-induced choroidal neovascularization (CNV) mouse model. Through integrative analysis and protein-protein interaction (PPI) analysis, common pathways and key transcription factor was identified simultaneously engaged in age-related retinal degeneration and CNV, the two typical pathological process of AMD. Subsequently, the expression changes of Spi1, the key regulator, as well as the alternation of the downstream mechanisms were validated in both models through qRT-PCR, Elisa, flow cytometry and immunofluorescence. Further, we assessed the impact of Spi1 knockdown in vitro and in vivo using gene intervention vectors carried by adeno-associated virus or lentivirus to test its potential as a therapeutic target.

Results

Compared to corresponding controls, we found 1,939 and 1,319 genes differentially expressed in eye cups of old and CNV mice respectively. The integrative analysis identified a total of 275 overlapping DEGs, of which 150 genes were co-upregulated. PPI analysis verified a central transcription factor, SPI1. The significant upregulation of Spi1 expression was then validated in both models, accompanied by macrophage polarization towards the M1 phenotype. Finally, SPI1 suppression significantly inhibited M1 polarization of BMDMs and attenuated neovascularization in CNV mice.

Conclusion

This study demonstrates that SPI1 exerts a pivotal role in AMD by regulation of macrophage polarization and innate immune response, offering promise as an innovative target for treating AMD.
Methodology for prioritizing marine environmental pressures under various management scenarios in the Black Sea
Authors: Lazar Luminita , Spanu Alina , Boicenco Laura , Oros Andra , Damir Nicoleta , Bisinicu Elena , Abaza Valeria , Filimon Adrian , Harcota George , Marin Oana , Pantea Elena , Timofte Florin , Vlas Oana , Korpinen Samuli
Published: June 2024

This study aims to develop a methodology for identifying predominant pressures on the marine ecosystem, emphasizing the significance of examining these pressures and the necessity for management scenarios. The research focuses on how the Black Sea ecosystem responds to the combined effects of human pressures, climate change, and policies. An in-depth analysis was conducted on environmental pressures affecting the Romanian Black Sea, highlighting dominant pressures such as physical habitat loss, hydrocarbon introduction, and non-indigenous species invasion. The research employs a novel methodological approach to assess the implications of these pressures under different Shared Socioeconomic Pathways (SSPs): SSP1 “Taking the Green Road”, SSP2 “Middle of the Road”, and SSP5 “Taking the Highway”. The findings reveal a complex interplay between economic development and environmental conservation, with each pathway presenting distinct outcomes for marine ecosystems. Recent developments, including beach rehabilitation, maritime transport, and oil and gas exploitation, have overshadowed traditional pressures such as nutrient introduction and fishing. The study identifies the increasing vulnerability of critical habitats to anthropogenic pressures, with the rehabilitation of these ecosystems remaining challenging even under reduced pressures. The results underscore the need for adaptive management strategies to enhance the Black Sea ecosystem’s sustainability and resilience. The study’s insights are important for developing management strategies that address ongoing environmental challenges. This research provides knowledge for policymakers and stakeholders involved in marine management and conservation efforts in the Black Sea region, emphasizing the importance of adaptive strategies to mitigate the adverse effects of human activities and climate change on marine ecosystems.