bluuebunny/crossref_metadata_2025 huggingface dataset:
LncRNA MHRT Prevents Angiotensin II-Induced Myocardial Oxidative Stress and NLRP3 Inflammasome via Nrf2 Activation
Authors: Liu Pinyi , Dong Xiaoming , Dong Chao , Hou Guowen , Liu Wenyun , Jiang Xin , Xin Ying
Published: March 2023

The development of angiotensin II (Ang II)-induced cardiomyopathies is reportedly mediated via oxidative stress and inflammation. Nuclear factor erythroid 2-related factor (Nrf2) is an important regulator of cellular antioxidant defense, and reactive oxygen species (ROS) can activate the NLRP3 inflammasome. MHRT is a newly discovered lncRNA exhibiting cardioprotective effects, demonstrated by inhibiting myocardial hypertrophy via Brg1 and myocardial apoptosis via Nrf2 upregulation. However, the underlying mechanism of MHRT remains unclear. We explored the potential protective effects of MHRT against Ang II-induced myocardial oxidative stress and NLRP3-mediated inflammation by targeting Nrf2. Chronic Ang II administration induced NLRP3 inflammasome activation (increased NLRP3, caspase-1 and interleukin-1β expression), oxidative stress (increased 3-nitrotyrosine and 4-hydroxy-2-nonenal), cardiac dysfunction and decreased MHRT and Nrf2 expression. Lentivirus-mediated MHRT overexpression inhibited Ang II (100 nM)-induced oxidative stress and NLRP3 inflammasome activation in AC16 human cardiomyocyte cells. Mechanistically, MHRT overexpression upregulated the expression and function of Nrf2, as determined by the increased transcription of downstream genes HO-1 and CAT, subsequently decreasing intracellular ROS accumulation and inhibiting the expression of thioredoxin-interacting protein (NLRP3 activator) and its direct binding to NLRP3. Accordingly, MHRT could protect against Ang II-induced myocardial injury by decreasing oxidative stress and NLRP3 inflammasome activation via Nrf2 activation.
Lightweight Tennis Ball Detection Algorithm Based on Robomaster EP
Authors: Zhao Yuan , Lu Ling , Yang Wu , Li Qizheng , Zhang Xiujie
Published: March 2023

To address the problems of poor recognition effect, low detection accuracy, many model parameters and computation, complex network structure, and unfavorable portability to embedded devices in traditional tennis ball detection algorithms, this study proposes a lightweight tennis ball detection algorithm, YOLOv5s-Z, based on the YOLOv5s algorithm and Robomater EP. The main work is as follows: firstly, the lightweight network G-Backbone and G-Neck network layers are constructed to reduce the number of parameters and computation of the network structure. Secondly, convolutional coordinate attention is incorporated into the G-Backbone to embed location information into channel attention, which enables the network to obtain location information of a larger area through multiple convolutions and enhances the expression ability of mobile network learning features. In addition, the Concat module in the original feature fusion is modified into a weighted bi-directional feature pyramid W-BiFPN with settable learning weights to improve the feature fusion capability and achieve efficient weighted feature fusion and bi-directional cross-scale connectivity. Finally, the Loss function EIOU Loss is introduced to split the influence factor of the aspect ratio and calculate the length and width of the target frame and anchor frame, respectively, combined with Focal-EIOU Loss to solve the problem of imbalance between complex and easy samples. Meta-ACON’s activation function is introduced to achieve an adaptive selection of whether to activate neurons and improve the detection accuracy. The experimental results show that compared with the YOLOv5s algorithm, the YOLOv5s-Z algorithm reduces the number of parameters and computation by 42% and 44%, respectively, reduces the model size by 39%, and improves the mean accuracy by 2%, verifying the effectiveness of the improved algorithm and the lightweight of the model, adapting to Robomaster EP, and meeting the deployment requirements of embedded devices for the detection and identification of tennis balls.
Experimental Investigation on Shear Behavior of Dune Sand Reinforced Concrete Deep Beams
Authors: Guo Zheyi , Qin Yongjun , Zhang Yifei , Li Xiangyang
Published: March 2023

An experimental study on the shear behavior of dune sand reinforced concrete (DSRC) deep beams was conducted to determine the feasibility of using dune sand (DS) in engineering. Nine DSRC deep beams were designed and thoroughly analyzed for failure modes, diagonal cracks, and load–deflection curves in this study. The results showed that the shear strength and ductility of DSRC deep beams increased when the DS replacement rate was 30%, but the opposite effect occurred when the DS replacement rate was 50%. To analyze the differences in the effects of the DS replacement rate, shear span-to-depth ratio, concrete strength, and stirrup ratio on the shear strength of DSRC and normal reinforced concrete (NRC) deep beams, a total of 227 shear experimental tests of NRC deep beams were conducted. Furthermore, four national codes were evaluated and compared based on experimental data. The evaluation results showed that the four codes underestimated the shear strength of DSRC and NRC deep beams. Among them, ACI 318–11 provided more reliable predictions for both NRC and DSRC deep beams. It is in this regard that a new empirical model for predicting the shear strength of DSRC deep beams is proposed, in which a reduction coefficient of the DS replacement rate is incorporated. The verification results indicates that the predicted results of the proposed model are in good agreement with the experimental results.
Efficient Processing of k-Hop Reachability Queries on Directed Graphs
Authors: Tang Xian , Zhou Junfeng , Shi Yunyu , Liu Xiang , Lin Keng
Published: March 2023

Given a directed graph, a k-hop reachability query, u→?kv, is used to check for the existence of a directed path from u to v that has a length of at most k. Addressing k-hop reachability queries is a fundamental task in graph theory and has been extensively investigated. However, existing algorithms can be inefficient when answering queries because they require costly graph traversal operations. To improve query performance, we propose an approach based on a vertex cover. We construct an index that covers all reachability information using a small set of vertices from the input graph. This allows us to answer k-hop reachability queries without performing graph traversal. We propose a linear-time algorithm to quickly compute a vertex cover, S, which we use to develop a novel labeling scheme and two algorithms for efficient query answering. The experimental results demonstrate that our approach significantly outperforms the existing approaches in terms of query response time.
Waste Silt as Filler in Hot Mix Asphalt: A Laboratory Characterization
Authors: Solouki Abbas , Tataranni Piergiorgio , Tarsi Giulia , Sangiorgi Cesare
Published: March 2023

Several studies aimed to improve both the performance and environmental impact of asphalt pavements using waste and recycled materials as fillers. This study focused on the effect of untreated and thermally treated silt as a filler in hot mix asphalt (HMA). The silt used in the study was a byproduct from a local aggregate production plant in Bologna, Italy. Mineral and chemical analyses revealed that the waste silt required thermal treatment at 750 °C for 2 h. The study compared the use of calcined silt, untreated silt, and a common limestone filler in the production of asphalt mastics and HMA specimens. The rheological properties of the mastics were analyzed using frequency sweep and multiple stress creep recovery tests. The physical and mechanical characteristics of the HMAs were evaluated through the air voids content, Marshall stability and indirect tensile strength tests. Additionally, the water susceptibility and thermal sensitivity of the HMAs were evaluated through the indirect tensile strength ratio and indirect tensile stiffness modulus at different testing temperatures. The results showed that the addition of calcined silt had no significant effect on the rheological properties of the mastic or the optimal binder content. However, the samples produced with thermally treated silt showed the highest stiffness and resistance to rutting compared with the other samples. On the other hand, the addition of untreated silt slightly decreased the stiffness value of the samples. In conclusion, the use of waste silt as a filler has potential as a sustainable and eco-friendly solution for HMAs.
Effectiveness of Machine-Learning and Deep-Learning Strategies for the Classification of Heat Treatments Applied to Low-Carbon Steels Based on Microstructural Analysis
Authors: Muñoz-Rodenas Jorge , García-Sevilla Francisco , Coello-Sobrino Juana , Martínez-Martínez Alberto , Miguel-Eguía Valentín
Published: March 2023

This work aims to compare the effectiveness of different machine-learning techniques for the image classification of steel microstructures. For this, we use a set of samples of hypoeutectoid steels subjected to three heat treatments: annealing, quenching and quenching with tempering. Logically, the samples contain the typical constituents expected, and these are different for each treatment. Images are obtained by optical microscopy at 400× magnification and from different low-carbon steels to generate the data with some heterogeneity. Learning models are created with an image dataset for classification into three classes based on the respective heat treatments. Likewise, we develop two kinds of models by using, on the one hand, classical machine-learning methods based on the “bag of features” technique and, on the other hand, convolutional neural networks (CNN) with a transfer-learning approach by using GoogLeNet and ResNet50. We demonstrate the superiority of deep-learning techniques (CNN) over classical machine-learning methods.
The Growth Curve of Microbial Cultures: A Model for a Visionary Reappraisal
Authors: Schiraldi Alberto
Published: March 2023

A phenomenological model of planktonic microbial cultures, reported in previous papers, suggests that the whole growth progress seems planned by the microbial population since a pre-growth latency phase, during which the population level remains at its starting level. This model is in line with recent suggestions about the behavior of complex systems, as long as it allows for the gathering of the growth trends of a number of real batch cultures in a single master plot of reduced variables, in spite of their metabolic and physiological differences. One important issue of the model concerns the origin of the time scale for the microbes that can differ from that for the observer. The present paper reports some consequences of the model in view of its potential use in predictive microbiology and proposes an extension to the steady and decay phases of the culture evolution suggesting that, consistent with the assumptions about the growth phase, the decay occurs by a scan of the cell generation steps. This view leads to the conclusion that the steady phase between growth and decay trends actually corresponds to the loss of the oldest cell generations, which represents minor fractions of the microbial population. Such early decay is almost undetectable in a log scale, looking like a steady phase. To account for cases that show a broad maximum instead of an intermediate steady trend, a single continuous function, still related to the model, can describe the whole growth and decay trend of the microbial culture.
Acts of Unsettling: An Immersive Adaptation of Berger and Mohr’s A Seventh Man
Authors: Babbage Frances , Pinchbeck Michael
Published: March 2023

This article examines Michael Pinchbeck and Ollie Smith’s theatrical adaptation of A Seventh Man, the 1975 book by John Berger and photographer Jean Mohr studying the experience of migrant workers in Europe. Pinchbeck and Smith’s 2020 adaptation uses immersive performance strategies in dialogue with a multi-voiced, cross-disciplinary publication that itself aims to produce an immersive or ‘animated’ reader engagement. In this article, Babbage and Pinchbeck present source text and performance as examples of practice-as-research, referencing Nelson’s paradigm that establishes different modes of knowledge and points of connection- and dissension-between them. They discuss the book’s cross-disciplinarity and the attempt to reflect this in a new creative context that is spatiotemporal, embodied, social, visual, verbal and aural. The article’s theoretical context draws on writing by Barthes, Berger, Said and Sontag, applying Barthes’ notion of the studium and the punctum to reflect on the dramaturgical rendering of the source text’s ‘interruptive shocks’. Babbage and Pinchbeck argue that, in book and performance, the juxtaposition of different formal languages elicits an encounter with the material that is productively ‘unsettling’.
SOC Estimation Based on Combination of Electrochemical and External Characteristics for Hybrid Lithium-Ion Capacitors
Authors: Huang Xiaofan , Gao Renjie , Zhang Luyao , Lv Xinrong , Shu Shaolong , Tang Xiaoping , Wang Ziyao , Zheng Junsheng
Published: March 2023

Hybrid lithium-ion capacitors (HyLICs) have received considerable attention because of their ability to combine the advantages of high-energy lithium-ion batteries and high-power supercapacitors. State of charge (SOC) is the main factor affecting the practical application of HyLICs; therefore, it is essential to estimate the SOC accurately. In this paper, a partition SOC-estimation method that combines electrochemical and external characteristics is proposed. The discharge process of the HyLICs was divided into three phases based on test results of electrochemical characteristics. To improve the estimation accuracy and reduce the amount of calculation, the Extended Kalman Filter (EKF) method was applied for SOC estimation at the interval where the capacitor energy storage characteristics dominated, and the Ampere-hour (Ah) method was used to estimate the SOC at the interval where battery energy storage characteristics dominated. The proposed method is verified under different operating conditions. The experimental results show good agreement with the estimation results, which indicates that the proposed method can estimate the SOC of the HyLICs accurately.
The Effect of Cinnamon and Ginger Spices on Anthocyanins in Sweetened Roselle Beverages
Authors: Omoarukhe Esereosa D. , Harbourne Niamh , Jauregi Paula
Published: March 2023

This study explores the potential benefits of spices (cinnamon and ginger) on Roselle anthocyanins within a sweetened Roselle beverage matrix. Anthocyanins and other related properties of the beverage (colour, antioxidant capacity, total phenolics, and pH) were observed from the start and monitored for 30 days at accelerated storage conditions (40 °C). The sweeteners at the amounts used (80 g/L granulated sugar and 0.32 g/L Stevia Reb A) did not have a significant effect on the initial anthocyanin content in the beverage and did not significantly impact degradation. Upon the addition of spices to the sweetened beverage, ginger (1 g/L) did not result in significant changes, initially or during storage. However, following the addition of cinnamon (1 g/L) to the beverages (unsweetened and sweetened), an initial increase in the total phenolic and FRAP antioxidant activity in the Roselle beverages was observed; furthermore, it reduced the degradation of anthocyanins and improved colour stability during storage. This effect is postulated to be due to a co-pigmentation reaction or the acylation of anthocyanins with a complex formed from the reaction of glucose with the phenolic compounds contained in cinnamon.
Investigation of Solid Phase Microextraction Gas Chromatography–Mass Spectrometry, Fourier Transform Infrared Spectroscopy and 1H qNMR Spectroscopy as Potential Methods for the Authentication of Baijiu Spirits
Authors: Fitzgerald Neil , Edwards John C.
Published: March 2023

The baijiu spirit is often the focus of fraudulent activity due to the widely varying prices of the products. In this work, Solid Phase Microextraction Gas Chromatography (SPME GCMS), Fourier Transform Infrared (FTIR) Spectroscopy and 1H qNMR spectroscopy were evaluated as potential methods to authenticate baijiu samples. Data were collected for 30 baijiu samples produced by seven different distilleries. The data from the SPME GCMS and FTIR methods were treated by a Principal Component Analysis to identify clusters that would suggest chemical differences in the products from different distilleries. The results suggest that SPME GCMS has the potential to be a fully portable method for baijiu authentication. FTIR did not appear suitable for authentication but can be used to find the %ABV range of the sample. 1H quantitative NMR (1H qNMR) was utilized to quantify the ethanol concentrations and calculate the observable congener chemistry comprising ester, ethanol, methanol, fusel alcohol, and organic acids. Discrepancies in ethanol content were observed in three samples, and a lack of major congeners in two samples indicates the possible presence of a counterfeit product. Detailed and quantitative congener chemistry is obtainable by NMR and provides a possible fingerprint analysis for the authentication and quality control of baijiu style, producer, and the length of the ageing process.
The Formulation of the N-Acetylglucosamine as Nanoparticles Increases Its Anti-Inflammatory Activities: An In Vitro Study
Authors: Mariano Alessia , Bigioni Irene , Ammendola Sergio , Scotto d’Abusco Anna
Published: March 2023

Nanomedicine can represent a new strategy to treat several types of diseases such as those with inflammatory aetiology. Through this strategy, it is possible to obtain nanoparticles with controlled shape, size, and eventually surface charge. Moreover, the use of molecules in nanoform may allow more effective delivery into the diseased cells and tissues, reducing toxicity and side effects of the used compounds. The aim of the present manuscript was the evaluation of the effects of N-acetylglucosamine in nanoform (GlcNAc NP) in an in vitro model of osteoarthritis (OA). Human primary chondrocytes were treated with Tumor Necrosis Factor (TNF)-α to simulate a low-grade inflammation and then treated with both GlcNAc and GlcNAc NP, in order to find the lowest concentrations able to counteract the inflammatory state of the cells and ensure a chondroprotective action. The findings showed that GlcNAc NP was able to decrease the pro-inflammatory mediators, IL-6 and IL-8, which are among the main effectors of inflammation; moreover, the nanoparticles downregulated the production of metalloprotease enzymes. GlcNAc NP was effective at a very low concentration compared to GlcNAc in its native form. Furthermore, GlcNAc NP stimulated an increase in collagen type II synthesis. In conclusion, the GlcNAc in nanoform showed better performance than GlcNAc, at concentrations lower than those reached in the joints after oral administration to patients of 1.5 g/die of glucosamine.
An Overview of Selected Material Properties in Finite Element Modeling of the Human Femur
Authors: Bazyar Pourya , Baumgart Andreas , Altenbach Holm , Usbeck Anna
Published: March 2023

Specific finite detail modeling of the human body gives a capable primary enhancement to the prediction of damage risk through automobile impact. Currently, car crash protection countermeasure improvement is based on an aggregate of testing with installed anthropomorphic test devices (i.e., ATD or dummy) and a mixture of multibody (dummy) and finite element detail (vehicle) modeling. If an incredibly easy finite element detail version can be advanced to capture extra statistics beyond the abilities of the multi-body structures, it might allow advanced countermeasure improvement through a more targeted prediction of overall performance. Numerous research has been done on finite element analysis of broken femurs. However, there are two missing pieces of information: 1- choosing the right material properties, and 2- designing a precise model including the inner structure of the bone. In this research, most of the chosen material properties for femur bone will be discussed and evaluated.
Mitochondrial DNA Polymorphism in HV1 and HV2 Regions and 12S rDNA in Perimenopausal Hypertensive Women
Authors: Kwaśniewski Wojciech , Stupak Aleksandra , Warowicka Alicja , Goździcka-Józefiak Anna , Mosiewicz Jerzy , Mieczkowska Jolanta
Published: March 2023

Estrogens enhance cellular mitochondrial activity. The diminution of female hormones during menopause may have an effect on the mitochondrial genome and the expression of mitochondrial proteins. Hence, oxidative stress and the pro-inflammatory state contribute to the formation of systemic illnesses including arterial hypertension (AH). This study aimed to determine the types and frequency of mutations in the mitochondrial DNA (mtDNA) nucleotide sequence in the hypervariable regions 1 and 2 (HV1 and HV2) and the 12S RNA coding sequence of the D-loop in postmenopausal women with hypertension. In our study, 100 women were investigated, 53 of whom were postmenopausal and 47 of whom were premenopausal (53.9 ± 3.7 years vs. 47.7 ± 4.2 years, respectively). Of those studied, 35 premenopausal and 40 postmenopausal women were diagnosed with AH. A medical checkup with 24 h monitoring of blood pressure (RR) and heart rate was undertaken (HR). The polymorphism of the D-loop and 12S rDNA region of mtDNA was examined. Changes in the nucleotide sequence of mtDNA were observed in 23% of the group of 100 women. The changes were identified in 91.3% of HV1 and HV2 regions, 60.9% of HV1 segments, 47.5% of HV2 regions, and 43.5% of 12S rDNA regions. The frequency of nucleotide sequence alterations in mtDNA was substantially higher in postmenopausal women (34%) than in premenopausal women (10.6%), p = 0.016. A higher frequency of changes in HV1 + HV2 sections in postmenopausal women (30.2%) compared to the premenopausal group (10.6%) was detected, p = 0.011. Only postmenopausal women were found to have modifications to the HV2 segment and the 12S rDNA region. After menopause, polymorphism in the mtDNA region was substantially more frequent in women with arterial hypertension than before menopause (p = 0.030; 37.5% vs. 11.5%). Comparable findings were observed in the HV2 and HV1 regions of the AH group (35% vs. 11.5%), p = 0.015, in the HV1 segment (25% vs. 11.5%), p = 0.529, and in the HV2 segment, 12S rDNA (25% vs. 0%). More than 80% of all changes in nucleotide sequence were homoplasmic. The mtDNA polymorphisms of the nucleotide sequence in the HV1 and HV2 regions, the HV2 region alone, and the 12S RNA coding sequence were associated with estrogen deficiency and a more severe course of arterial hypertension, accompanied by symptoms of adrenergic stimulation.
Carotenoids: Role in Neurodegenerative Diseases Remediation
Authors: Gandla Kumaraswamy , Babu Ancha Kishore , Unnisa Aziz , Sharma Indu , Singh Laliteshwar Pratap , Haque Mahammad Akiful , Dashputre Neelam Laxman , Baig Shahajan , Siddiqui Falak A. , Khandaker Mayeen Uddin , Almujally Abdullah , Tamam Nissren , Sulieman Abdelmoneim , Khan Sharuk L. , Emran Talha Bin
Published: March 2023

Numerous factors can contribute to the development of neurodegenerative disorders (NDs), such as Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis, Huntington’s disease, and multiple sclerosis. Oxidative stress (OS), a fairly common ND symptom, can be caused by more reactive oxygen species being made. In addition, the pathological state of NDs, which includes a high number of protein aggregates, could make chronic inflammation worse by activating microglia. Carotenoids, often known as “CTs”, are pigments that exist naturally and play a vital role in the prevention of several brain illnesses. CTs are organic pigments with major significance in ND prevention. More than 600 CTs have been discovered in nature, and they may be found in a wide variety of creatures. Different forms of CTs are responsible for the red, yellow, and orange pigments seen in many animals and plants. Because of their unique structure, CTs exhibit a wide range of bioactive effects, such as anti-inflammatory and antioxidant effects. The preventive effects of CTs have led researchers to find a strong correlation between CT levels in the body and the avoidance and treatment of several ailments, including NDs. To further understand the connection between OS, neuroinflammation, and NDs, a literature review has been compiled. In addition, we have focused on the anti-inflammatory and antioxidant properties of CTs for the treatment and management of NDs.
Relationship between Corticospinal Excitability While Gazing at the Mirror and Motor Imagery Ability
Authors: Iwanami Jun , Mutai Hitoshi , Sagari Akira , Sato Masaaki , Kobayashi Masayoshi
Published: March 2023

Mirror therapy (MT) helps stroke survivors recover motor function. Previous studies have reported that an individual’s motor imagery ability is related to the areas of brain activity during motor imagery and the effectiveness of motor imagery training. However, the relationship between MT and motor imagery ability and between corticospinal tract excitability during mirror gazing, an important component of MT, and motor imagery ability is unclear. This study determined whether the motor-evoked potential (MEP) amplitude while gazing at the mirror relates to participants’ motor imagery abilities. Twenty-four healthy right-handed adults (seven males) were recruited. Transcranial magnetic stimulation was performed while gazing at the mirror, and MEP of the first dorsal interosseous muscle of the right hand were measured. Motor imagery ability was measured using the Kinesthetic and Visual Imagery Questionnaire (KVIQ), which assesses the vividness of motor imagery ability. Additionally, a mental chronometry (MC) task was used to assess time aspects. The results showed a significant moderate correlation between changes in MEP amplitude values while gazing at the mirror, as compared with resting conditions, and assessment scores of KVIQ. This study shows that corticospinal excitability because of mirror gazing may be related to the vividness of motor imagery ability.
HPV-Based Self-Sampling in Cervical Cancer Screening: An Updated Review of the Current Evidence in the Literature
Authors: Daponte Nikoletta , Valasoulis George , Michail Georgios , Magaliou Ioulia , Daponte Athina-Ioanna , Garas Antonios , Grivea Ioanna , Bogdanos Dimitrios P. , Daponte Alexandros
Published: March 2023

Identifying and reaching women at higher risk for cervical cancer is all-important for achieving the ambitious endpoints set in 2020 by the WHO for global cervical cancer control by 2030. HPV-based (vaginal) self-sampling (SS) represents a cost-effective screening strategy, which has been successfully implemented during the last decade both in affluent and constrained settings. Among other advantages, SS strategies offer convenience, diminished costs, flexibility to obtain a sample in the office or home, avoiding a pelvic exam and uncomfortable appointment with a healthcare professional, as well as social and cultural acceptability. SS implementation has been globally boosted during the COVID-19 pandemic. In pragmatic terms, social distancing, local lockdowns, discontinuation of clinics and reallocation of human and financial resources challenged established clinician-based screening; self-collection strategies apparently surpassed most obstacles, representing a viable and flexible alternative. With time, sufficient reassuring data has accumulated regarding specially designed SS devices, aspects of sample preparation, transport and storage and, importantly, optimization of validated PCR-based HPV testing platforms for self-collected specimens. Suboptimal rates of clinical follow-up post-SS screening, as well as overtreatment with reliance solely on molecular assays, have both been documented and remain concerning. Therefore, effective strategies are still required to ensure linkage to follow-up testing and management following positive SS results by trained health professionals with knowledge of HPV biology and management algorithms. Because of the prolonged SS screening intervals, implementation data are limited regarding subsequent screening rounds of SS-screened individuals; however, these are accumulating gradually. With further refinement of assays and validation of novel biomarkers in self-collected samples, there is a clear potential for increasing SS accuracy and PPV. The potential differentiation of self-collection protocols for vaccinated versus non-vaccinated individuals also represents an open issue. In conclusion, HPV-based self-collection techniques can effectively address limited uptake alongside other conventional cervical screening drawbacks; however, assays, logistics and infrastructures need further optimization to increase the efficacy, effectiveness and cost-effectiveness of SS approaches.
Is It Time to Integrate Frailty Assessment in Onconephrology?
Authors: Wu Henry H. L. , Chinnadurai Rajkumar , Walker Robert J. , Tennankore Karthik K.
Published: March 2023

Onconephrology has emerged as a novel sub-specialty of nephrology dedicated to the intersection between the kidney and cancer. This intersection is broad and includes a number of important areas of focus, including concurrent chronic kidney disease (CKD) and cancer, acute kidney complications of cancer, and cancer-treatment-induced nephrotoxicity. The importance of onconephrology is even more evident when considering the global growth in the population of older adults, many of whom are living with some degree of frailty. Furthermore, a considerable proportion of older adults have CKD (some of whom eventually progress to kidney failure) and are at high risk of developing solid tumour and hematologic malignancies. Specific to kidney disease, the association between frailty status and kidney disease has been explored in depth, and tools to capture frailty can be used to guide the management and prognostication of older adults living with kidney failure. Whilst there is emerging data regarding the assessment and impact of frailty in onconephrology, there remains a relative paucity of knowledge within this topic. In this article, we evaluate the definition and operationalization of frailty and discuss the significance of frailty within onconephrology. We review evidence on current approaches to assessing frailty in onconephrology and discuss potential developments and future directions regarding the utilization of frailty in this patient population. A greater awareness of the intersections and interactions between frailty and onconephrology and further efforts to integrate frailty assessment in onconephrology to optimize the delivery of realistic and goal-directed management strategies for patients is needed.
Design of a New Chemoenzymatic Process for Producing Epoxidized Monoalkyl Esters from Used Soybean Cooking Oil and Fusel Oil
Authors: Mattos Fernanda R. , Júnior José Miguel , Sabi Guilherme J. , Garcia Pedro H. D. , Carvalho Patrícia O. , Luiz Jaine H. H. , Mendes Adriano A.
Published: March 2023

The aim of this study was to produce epoxidized monoalkyl esters (EMAE), a valuable class of oleochemicals used in a wide range of products and industries, from used soybean cooking oil (USCO) and fusel oil via a three-step chemoenzymatic process. This process consists of a first enzymatic hydrolysis of USCO to produce free fatty acids (FFA). Here, five microbial lipases with different specificities were tested as biocatalysts. Full hydrolysis of USCO was obtained after a 180 min reaction time under vigorous stirring (1500 rpm) using a non-specific lipase from Candida rugosa (CRL). Then, monoalkyl esters (MAE) were produced via the esterification of FFA and fusel oil in a solvent-free system using the lipase Eversa® Transform 2.0 (ET2.0) immobilized via physical adsorption on poly(styrenene-divinylbenzene) (PSty-DVB) beads as a biocatalyst. Different water removal strategies (closed and open reactors in the presence or absence of molecular sieves at 5% m.m−1) on the reaction were evaluated. Maximum FFA conversions of 64.3 ± 2.3% (open reactor after a 30 min reaction time) and 73.5 ± 0.4% (closed reactor after a 45 min reaction time) were observed at 40 °C, using a stoichiometric FFA:fusel oil molar ratio (1:1), without molecular sieves, and 5 mg of immobilized protein per gram of reaction mixture. Under these conditions, maximum FFA conversion was only 30.2 ± 2.7% after a 210 min reaction time in a closed reactor using soluble lipase. Reusability tests showed better retention of the original activity of immobilized ET2.0 (around 82%) after eight successive batches of esterification reactions conducted in an open reactor. Finally, the produced MAE was epoxidized via the Prilezhaev reaction, a classical chemical epoxidation process, using hydrogen peroxide and formic acid as a homogeneous catalyst. The products were characterized by standard methods and identified using proton nuclear magnetic resonance (1H NMR). Maximum unsaturated bond conversions into epoxy groups were at approximately 33%, with the experimental epoxy oxygen content (OOCexp.) at 1.75–1.78%, and selectivity (S) at 0.81, using both MAEs produced (open or closed reactors). These results show that this new process is a promising approach for value-added oleochemical production from low-cost and renewable raw materials.
Targeting Post-Translational Modifications to Improve Combinatorial Therapies in Breast Cancer: The Role of Fucosylation
Authors: Antonarelli Gabriele , Pieri Valentina , Porta Francesca Maria , Fusco Nicola , Finocchiaro Gaetano , Curigliano Giuseppe , Criscitiello Carmen
Published: March 2023

Various tumors rely on post-translational modifications (PTMs) to promote invasiveness and angiogenesis and to reprogram cellular energetics to abate anti-cancer immunity. Among PTMs, fucosylation is a particular type of glycosylation that has been linked to different aspects of immune and hormonal physiological functions as well as hijacked by many types of tumors. Multiple tumors, including breast cancer, have been linked to dismal prognoses and increased metastatic potential due to fucosylation of the glycan core, namely core-fucosylation. Pre-clinical studies have examined the molecular mechanisms regulating core-fucosylation in breast cancer models, its negative prognostic value across multiple disease stages, and the activity of in vivo pharmacological inhibition, instructing combinatorial therapies and translation into clinical practice. Throughout this review, we describe the role of fucosylation in solid tumors, with a particular focus on breast cancer, as well as physiologic conditions on the immune system and hormones, providing a view into its potential as a biomarker for predicating or predicting cancer outcomes, as well as a potential clinical actionability as a biomarker.
Complete Freund’s Adjuvant Induces a Fibroblast-like Synoviocytes (FLS) Metabolic and Migratory Phenotype in Resident Fibroblasts of the Inoculated Footpad at the Earliest Stage of Adjuvant-Induced Arthritis
Authors: González-Chávez Susana Aideé , Chaparro-Barrera Eduardo , Alvarado-Jáquez María Fernanda , Cuevas-Martínez Rubén , Ochoa-Albíztegui Rosa Elena , Pacheco-Tena César
Published: March 2023

The fibroblast-like synoviocytes (FLS) have a crucial role in the pathogenesis of Rheumatoid Arthritis (RA); however, its precise mechanisms remain partially unknown. The involvement of the fibroblast in activating adjuvant-induced arthritis (AA) has not been previously reported. The objective was to describe the participation of footpads’ fibroblasts in the critical initial process that drives the AA onset. Wistar rats were injected with Complete Freund’s Adjuvant (CFA) or saline solution in the hind paws’ footpads and euthanized at 24 or 48 h for genetic and histological analyses. Microarrays revealed the differentially expressed genes between the groups. The CFA dysregulated RA-linked biological processes at both times. Genes of MAPK, Jak-STAT, HIF, PI3K-Akt, TLR, TNF, and NF-κB signaling pathways were altered 24 h before the arrival of immune cells (CD4, CD8, and CD68). Key markers TNF-α, IL-1β, IL-6, NFκB, MEK-1, JAK3, Enolase, and VEGF were immunodetected in fibroblast in CFA-injected footpads at 24 h but not in the control group. Moreover, fibroblasts in the CFA inoculation site overexpressed cadherin-11, which is linked to the migration and invasion ability of RA-FLS. Our study shows that CFA induced a pathological phenotype in the fibroblast of the inoculation site at very early AA stages from 24 h, suggesting a prominent role in arthritis activation processes.
Cannabinoid Receptor 1 Agonist ACEA and Cannabinoid Receptor 2 Agonist GW833972A Attenuates Cell-Mediated Immunity by Different Biological Mechanisms
Authors: Takheaw Nuchjira , Jindaphun Kanyaruck , Pata Supansa , Laopajon Witida , Kasinrerk Watchara
Published: March 2023

Cannabinoid receptor 1 (CB1) and cannabinoid receptor 2 (CB2) are components in the endocannabinoid system that play significant roles in regulating immune responses. There are many agonists for the cannabinoid receptors; however, their effects on T cell regulation have not been elucidated. In the present study, we determined the effects of the CB1 selective agonist ACEA and the CB2 selective agonist GW833972A on T cell responses. It was found that both agonists impaired anti-CD3 monoclonal antibody induced T cell proliferation. However, ACEA and GW833972A agonists down-regulated the expression of activation markers on CD4+ and CD8+ T cells and co-stimulatory molecules on B cells and monocytes in different manners. Moreover, only GW833972A suppressed the cytotoxic activities of CD8+ T cells without interfering in the cytotoxic activities of CD4+ T cells and NK cells. In addition, the CB2 agonist, but not CB1 agonist, caused the reduction of Th1 cytokine production. Our results demonstrated that the CB1 agonist ACEA and CB2 agonist GW833972A attenuated cell-mediated immunity in different mechanisms. These agonists may be able to be used as therapeutic agents for inducing T cell hypofunction in inflammatory and autoimmune diseases.
Identification of a miRNA Panel with a Potential Determinant Role in Patients Suffering from Periodontitis
Authors: Baru Oana , Raduly Lajos , Bica Cecilia , Chiroi Paul , Budisan Liviuta , Mehterov Nikolay , Ciocan Cristina , Pop Laura Ancuta , Buduru Smaranda , Braicu Cornelia , Badea Mandra , Berindan-Neagoe Ioana
Published: March 2023

In recent years, the role of microRNA (miRNA) in post-transcriptional gene regulation has advanced and supports strong evidence related to their important role in the regulation of a wide range of fundamental biological processes. Our study focuses on identifying specific alterations of miRNA patterns in periodontitis compared with healthy subjects. In the present study, we mapped the major miRNAs altered in patients with periodontitis (n = 3) compared with healthy subjects (n = 5), using microarray technology followed by a validation step by qRT-PCR and Ingenuity Pathways Analysis. Compared to healthy subjects, 159 differentially expressed miRNAs were identified among periodontitis patients, of which 89 were downregulated, and 70 were upregulated, considering a fold change of ±1.5 as the cut-off value and p ≤ 0.05. Key angiogenic miRNAs (miR-191-3p, miR-221-3p, miR-224-5p, miR-1228-3p) were further validated on a separate cohort of patients with periodontitis versus healthy controls by qRT-PCR, confirming the microarray data. Our findings indicate a periodontitis-specific miRNA expression pattern representing an essential issue for testing new potential diagnostic or prognostic biomarkers for periodontal disease. The identified miRNA profile in periodontal gingival tissue was linked to angiogenesis, with an important molecular mechanism that orchestrates cell fate.
Graphene Embedded with Transition Metals for Capturing Carbon Dioxide: Gas Detection Study Using QM Methods
Authors: Mollaamin Fatemeh , Monajjemi Majid
Published: March 2023

Carbon dioxide (CO2) adsorption on decorated graphene (GR) sheets with transition metals (TMs) including iron, nickel and zinc was investigated for removing this hazardous gas from the environment. TM-doped GR results in higher activity toward gas detecting than pristine graphene nanosheets. TM embedding restrains hydrogen evolution on the C sites, leaving more available sites for a CO2 decrease. The Langmuir adsorption model with ONIOM using CAM-B3LYP functional and LANL2DZ and 6-31+G (d,p) basis sets due to Gaussian 16 revision C.01 program on the complexes of CO2→(Fe, Ni, Zn) embedded on the GR was accomplished. The changes of charge density illustrated a more considerable charge transfer for Zn-embedded GR. The thermodynamic results from IR spectroscopy indicated that ΔGads,CO2→Zn@C−GRo has the notable gap of Gibbs free energy adsorption with a dipole moment which defines the alterations between the Gibbs free energy of the initial compounds (ΔGCO2 o and ΔGZn@C−GRo) and product compound (ΔGCO2→Zn@C−GRo) through polarizability. Frontier molecular orbital and band energy gaps accompanying some chemical reactivity parameters represented the behavior of molecular electrical transport of the (Fe, Ni, Zn) embedding of GR for the adsorption of CO2 gas molecules. Our results have provided a favorable understanding of the interaction between TM-embedded graphene nanosheets and CO2.
In Vitro Photoprotection and Functional Photostability of Sunscreen Lipsticks Containing Inorganic Active Compounds
Authors: da Silva Marcelino Priscila , Martinez Renata Miliani , Daneluti André Luís Maximo , Morocho-Jácome Ana Lucía , Pessoa Fabiana Vieira Lima Solino , Rijo Patrícia , Rosado Catarina , Velasco Maria Valeria Robles , Baby André Rolim
Published: March 2023

Titanium dioxide (TiO2) is a safe inorganic ultraviolet (UV) filter with activity against UV damage. However, the recombination of the carrier’s charge and the tendency for TiO2 aggregation are the main disadvantages. Substrate supports, such as mesoporous silica, are biocompatible strategies to incorporate TiO2, altering its interaction with the skin. Since the lips are sensitive to the adversities of the environment, including UV radiation, the application of lipstick sunscreens is of great importance and expected to provide protection for this particular area against sunburn and photoaging, among other unfavorable responses unprotected UV exposure. We investigated the in vitro photoprotective efficacy and photostability of lipstick formulations containing TiO2 incorporated into mesoporous silica (SBA-15). The samples were the lipstick base; SBA-15; TiO2 (free form); and TiO2 incorporated into SBA-15. The photoprotective efficacy was characterized in vitro using a Labsphere UV2000S. Lipsticks were irradiated in a Suntest CPS+ chamber to evaluate functional photostability. Lipstick base and SBA-15 alone did not display photoprotective efficacy. The sample containing 10.0% TiO2 incorporated into the mesoporous silica generated greater photostability and sun protection factor (SPF) value compared to the one containing only 10.0% TiO2 (free state). Our findings suggest that TiO2 + SBA-15 can be considered a broad-spectrum ingredient for innovative sunscreens, particularly for the photoprotection of the lips.
It Didn’t Go Away: The Political and Social Determinants of COVID-19 Mortality Rates across Counties in the United States
Authors: Rambotti Simone , Wolski Caroline , Anderson Kathryn Freeman
Published: March 2023

Research over the last several years has demonstrated a wide variety of inequalities in the COVID-19 pandemic by socio-demographic characteristics, place, and political and religious ideology. In this study, by combining several county-level data sources, we examine how the social conditions of counties across the United States relate to their differential COVID-19 mortality rates. We find that percent Black, percent Hispanic, and income inequality are all positively related to higher mortality rates at the county level. Moreover, the percentage of the population that voted for Trump in the 2020 election was a significant and substantively large predictor of higher mortality rates. We also include healthcare-related variables, but compared to the social circumstances of the pandemic, these effects are relatively small. These results indicate that the social conditions of areas are strong predictors of how counties have experienced the pandemic and where the greatest loss of life has occurred.
Analysis on Cascading Failures of Directed–Undirected Interdependent Networks with Different Coupling Patterns
Authors: Xu Xiaojie , Fu Xiuwen
Published: March 2023

Most existing studies model interdependent networks as simple network systems consisting of two or more undirected subnets, and the interdependent edges between the networks are undirected. However, many real-world interdependent networks are coupled by a directed subnet and an undirected subnet, such as supply chain networks coupled with cyber networks, and cyber manufacturing networks coupled with service networks. Therefore, in this work, we focus on a ubiquitous type of interdependent network—the directed–undirected interdependent network—and research the cascading failures of directed–undirected interdependent networks with different coupling patterns. Owing to the diversity of coupling patterns to realistic interdependent network systems, we introduce two types of interdependent edges (i.e., directed-to-undirected and undirected-to-directed interdependent edges). On this basis, we generated different types of directed–undirected interdependent networks with varying coupling patterns (i.e., one-to-one, one-to-many, and many-to-one) and investigated the cascading failure robustness of these types of networks. Finally, we explored the cascading robustness of directed–undirected interdependent networks under two different attack strategies (single-node attack and multi-node attack). Through extensive experiments, we have obtained some meaningful findings: (1) the cascading robustness of directed–undirected interdependent networks is positively related to the overload tolerance coefficient and load exponential coefficient; (2) high-degree nodes and high-in-degree nodes should be protected to improve the cascading robustness of directed–undirected interdependent networks; (3) the cascading robustness of one-to-many interdependent networks can be improved by adding directed-to-undirected interdependent edges; and the cascading robustness of many-to-one interdependent networks can be improved by adding undirected-to-directed interdependent edges.
Airborne Quantum Key Distribution Performance Analysis under Supersonic Boundary Layer
Authors: Yu Huicun , Tang Bangying , Ding Haolin , Xue Yang , Tang Jie , Wang Xingyu , Liu Bo , Shi Lei
Published: March 2023

Airborne quantum key distribution (QKD) that can synergize with terrestrial networks and quantum satellite nodes is expected to provide flexible and relay links for the large-scale integrated communication network. However, the photon transmission rate would be randomly reduced, owing to the random distributed boundary layer that surrounding to the surface of the aircraft when the flight speed larger than Mach 0.3. Here, we investigate the airborne QKD performance with the BL effects. Furthermore, we take experimental data of supersonic BL into the model and compare the airborne QKD performance under different conditions. Simulation results show that, owing to the complex small-scale turbulence structures in the supersonic boundary layer, the deflection angle and correspondingly drifted offset of the beam varied obviously and randomly, and the distribution probability of photons are redistributed. And the subsonic and supersonic boundary layer would decrease ~35.8% and ~62.5% of the secure key rate respectively. Our work provides a theoretical guidance towards a possible realization of high-speed airborne QKD.