bluuebunny/crossref_metadata_2025 huggingface dataset:
Isolation, identification, and monoclonal antibody development of largemouth bass virus
Authors: Qin Yinghui , Liu Haixiang , Mao Shuangshuang , Deng Riying , Wang Yuhang , Deng Si , Zhang Peipei , Yao Lunguang
Published: January 2024

Largemouth bass virus (LMBV) poses a significant threat to largemouth bass farming, leading to substantial economic losses. In December 2022, massive largemouth bass juveniles died at a fish farm in the city of Xinxiang, China. Through a series of experiments, we conclusively identified LMBV as the causative pathogen. The affected fish displayed anorexia, lethargy, and hemorrhage at the pectoral and caudal fin base. No parasites or pathogenic bacteria were detected on the body surface or gills, or isolated from the diseased fish. Severe hemorrhage, lymphocyte infiltration, and extensive necrosis were observed in the liver, spleen, intestine, and stomach of the moribund fish. The tissue homogenate from the diseased fish induced epithelioma papulosum cyprini cells (EPC) cell death, while no such effects were observed in grouper spleen (GS) cells. Sequence similarity analysis of the major capsid protein (MCP) indicated the virus shared 100% similarity with the LMBV-FS2021 strain, placing it within the Ranavirus genus. Transmission electron microscopy (TEM) observations revealed plenty of hexagonal virions accumulated in the cytoplasm of infected EPC cells. Artificial infection demonstrated that LMBV-XX01 was highly fatal to Micropterus salmoides juveniles, with an LD50 of 103.081 TCID50/fish. RT-qPCR detection confirmed that LMBV appeared in all sampled tissues of the challenged largemouth bass, with significantly higher viral loads detected in the liver and heart compared to other tissues. Additionally, we successfully obtained a highly purified recombinant MCP of LMBV and developed two strains of monoclonal antibodies targeting MCP of LMBV-XX01. Overall, our findings provide valuable materials and insights for the design of prevention strategies and the development of detection methods for LMBV.
Molecular diagnosis of opportunistic infections in the central nervous system of HIV-infected adults in Manaus, Amazonas
Authors: de Melo Sabrina Araújo , Pinto Sérgio Damasceno , Ferreira Ewerton da Silva , Brotas Reinan , Marinho Eveny Perlize Melo , da Silva Valderjane Aprigio , Monte Rossiclea Lins , Feitoza Pablo Vinícius Silveira , Reis Monique Freire , Almeida Taynná V. Rocha , Ferreira Luiz Carlos de Lima , Bastos Michele de Souza
Published: January 2024

Background

Opportunistic infections in the central nervous system (CNS) of people with HIV/AIDS (PLWHA) remain significant contributors to morbidity and mortality, especially in resource-limited scenarios. Diagnosing these infections can be challenging, as brain imaging is non-specific and expensive. Therefore, molecular analysis of cerebrospinal fluid (CSF) may offer a more accurate and affordable method for diagnosing pathogens.

Methods

We conducted extensive real-time PCR testing (qPCR) on CSF to evaluate etiological agents in PLWHA with neurological manifestations. Primers targeting DNA from specific pathogens, including cytomegalovirus (CMV), herpes simplex virus (HSV), varicella-zoster virus (VZV), Epstein–Barr virus (EBV), John Cunningham virus (JCV), Toxoplasma gondii, and human T-lymphotropic virus types 1 and 2 (HTLV-1 and HTLV-2), were used.

Results

Cerebrospinal fluid samples revealed 90 pathogens (36.7%). Toxoplasma gondii was the most frequently detected pathogen, found in 22 samples (30.5%). Other pathogens included Cryptococcus sp. (7.7%), EBV (5.3%), CMV, VZV, and JCV (4.0% each).

Conclusion

Despite antiretroviral therapy and medical follow-up, opportunistic central nervous system infections remain frequent in PLWHA. Herpesviruses are commonly detected, but T. gondii is the most prevalent opportunistic pathogen in our study population. Therefore, molecular diagnosis is a crucial tool for identifying opportunistic infections, even in patients undergoing treatment.
Community structure and association network of prokaryotic community in surface sediments from the Bering-Chukchi shelf and adjacent sea areas
Authors: Xie Changliang , Ouyang Hong , Zheng Hu , Wang Maoting , Gu Junning , Wang Zhaohui , Tang Yali , Xiao Lijuan
Published: January 2024

The Bering-Chukchi shelf is one of the world’s most productive areas and characterized by high benthic biomass. Sedimentary microbial communities play a crucial role in the remineralization of organic matter and associated biogeochemical cycles, reflecting both short-term changes in the environment and more consistent long-term environmental characteristics in a given habitat. In order to get a better understanding of the community structure of sediment-associated prokaryotes, surface sediments were collected from 26 stations in the Bering-Chukchi shelf and adjacent northern deep seas in this study. Prokaryote community structures were analyzed by metabarcoding of the 16S rRNA gene, and potential interactions among prokaryotic groups were analyzed by co-occurrence networks. Relationships between the prokaryote community and environmental factors were assessed. Gammaproteobacteria, Alphaproteobacteria, and Flavobacteriia were the dominant bacterial classes, contributing 35.0, 18.9, and 17.3% of the bacterial reads, respectively. The phototrophic cyanobacteria accounted for 2.7% of the DNA reads and occurred more abundantly in the Bering-Chukchi shelf. Prokaryotic community assemblages were different in the northern deep seas compared to the Bering-Chukchi shelf, represented by the lowered diversity and the increased abundant operational Taxonomic Units (OTU), suggesting that the abundant taxa may play more important roles in the northern deep seas. Correlation analysis showed that latitude, water depth, and nutrients were important factors affecting the prokaryote community structure. Abundant OTUs were distributed widely in the study area. The complex association networks indicated a stable microbial community structure in the study area. The high positive interactions (81.8–97.7%) in this study suggested that symbiotic and/or cooperative relationships accounted for a dominant proportion of the microbial networks. However, the dominant taxa were generally located at the edge of the co-occurrence networks rather than in the major modules. Most of the keystone OTUs were intermediately abundant OTUs with relative reads between 0.01 and 1%, suggesting that taxa with moderate biomass might have considerable impacts on the structure and function of the microbial community. This study enriched the understanding of prokaryotic community in surface sediments from the Bering-Chukchi shelf and adjacent sea areas.
Airborne microbes in five important regions of Chinese traditional distilled liquor (Baijiu) brewing: regional and seasonal variations
Authors: Xu Yan , Qiao Xue , He Lei , Wan Wujie , Xu Zhongjun , Shu Xi , Yang Cheng , Tang Ya
Published: January 2024

Baijiu is one of the six primary distilled spirits in the world. It is produced through the solid-state fermentation of grains in the open environment, so high-quality Baijiu brewing largely depends on terrior. Environmental microbes are one of the most important factors affecting the quality, quantity, and flavors of Baijiu. As atmosphere is a pool and transport pathway for microbes from the ambient environment to Baijiu brewing ecosystems, we explored the functional microbes of Baijiu brewing in five important regions. The regions fell into two topographical types, namely, plain and river-valley. In total, 41 functional microbes were identified rich (relative abundance >0.1%) in at least one of the regions, such as the fungi of Aspergillus, Candida, Cladosporium, Debaryomyces, Penicillium, Pichia, Rhizopus, Saccharomyces, and Wickerhamomyces and the bacteria of Acetobacter, Bacillus, Clostridium, Enterobacter, Lactobacillus, Methanosarcina, Methanobacterium, Methanobrevibacter, and Pseudomonas. However, some functional bacteria (e.g., Clostridia, Gluconacetobacter, and Weissella) and fungi (e.g., Dekkera, Eurotium, Issatchenkia, Mucor, and Phoma) were not rich or were not detected in the atmosphere. Airborne microbiomes and the Phylogenetic Diversity (PD) index were significantly different between the main brewing season (winter) and the summer break in each region, except for the fungi in one region. In winter, airborne microbiomes were significantly different among almost all the regions. The relative abundance of bacterial fermentation function in each region increased from summer to winter. The relative abundances of fungal yeast function were higher in winter for the plain regions but were higher in summer for the river-valley regions. In sum, our results suggested that: (1) atmosphere was one but not the sole important source of functional microbes for Baijiu brewing and (2) microbiomes in different regions might be quite different but they could share some major functions related to Baijiu brewing.
Fasting diets: what are the impacts on eating behaviors, sleep, mood, and well-being?
Authors: Hosseini Elham , Ammar Achraf , Josephson Jessica K. , Gibson Deanna L. , Askari Gholamreza , Bragazzi Nicola L. , Trabelsi Khaled , Schöllhorn Wolfgang I. , Mokhtari Zeinab
Published: January 2024

Fasting diets (FDs) have drawn great attention concerning their contribution to health and disease over the last decade. Despite considerable interest in FDs, the effect of fasting diets on eating behaviors, sleep, and mood-essential components of diet satisfaction and mental health- has not been addressed comprehensively. Understanding the critical role that fasting plays in these elements will open up potential treatment avenues that have not yet been explored. The aim of the present paper was to conduct a comprehensive critical review exploring the effects of fasting on eating behaviors, sleep, and mood. There is currently a lack of clarity regarding which fasting option yields the most advantageous effects, and there is also a scarcity of consistent trials that assess the effects of FDs in a comparable manner. Similarly, the effects and/or treatment options for utilizing FDs to modify eating and sleep behaviors and enhance mood are still poorly understood. Further researches aiming at understanding the impacts of various fasting regimes, providing new insights into the gut-brain axis and offering new treatment avenues for those with resistant anxiety and depression, are warranted. Alteration of eating behaviors can have lasting effects on various physiological parameters. The use of fasting cures can underpin ancient knowledge with scientific evidence to form a new approach to the prevention and treatment of problems associated with co-morbidities or challenges pertaining to eating behaviors. Therefore, a thorough examination of the various fasting regimens and how they impact disease patterns is also warranted.
Sensory, textural, physico-chemical and enzymatic characterization of melted cheese with added potato and carrot peels
Authors: Tiţa Ovidiu , Constantinescu Maria Adelina , Tiţa Mihaela Adriana , Bătuşaru Cristina , Mironescu Ion
Published: January 2024

Food waste is one of the biggest societal problems in the globe due to its detrimental consequences on the environment. According to estimates from the Food and Agriculture Organization, this comes to about 1.3 billion tonnes per year. The current study aims to produce sustainable food products with high nutritional value by incorporating food waste. For the extraction of economically relevant products such as dietary fibers, biopolymers, natural antioxidants, and food additives, potato and carrot peel represent an inexpensive, valuable, and conveniently available resource. Cheese is a functional dairy product that people eat for its high nutritional content, which aids in the treatment of conditions including diabetes, obesity, hypertension, and digestive problems in addition to giving them energy. Thus a control sample and 10 samples of melted cheese with various amounts of potato and carrot peelings were prepared. To explore the nutritional value of potato and carrot peels in melted cheese, an analysis was conducted on the fluctuation of physicochemical (acidity, pH, dry matter, water activity, and salt content) and enzymatic (L-lactic acid, lactose, D-glucose, and D-galactose) parameters. Consumer acceptability of the products was assessed by textural and sensory analysis. During the whole storage period, the samples of melted cheese with potato and carrot peels recorded higher values than the control sample, the results obtained for them being better. Samples with added potato or carrot wastes were more stable over time, as compared to control samples.
Barriers to promoting breastfeeding in primary health care in Mexico: a qualitative perspective
Authors: Hoyos-Loya Elizabeth , Pérez Navarro Cecilia , Burrola-Méndez Soraya , Hernández-Cordero Sonia , Omaña-Guzmán Isabel , Sachse Aguilera Matthias , Ancira-Moreno Mónica
Published: January 2024

Objective

This article aimed to identify the main barriers related to promoting and counseling breastfeeding (BF) at the Primary Health Care (PHC) in Mexico.

Methodology

A qualitative study with a phenomenological approach was carried out in 88 health centers of the Ministry of Health in the states of Chihuahua, Oaxaca, Chiapas, Veracruz, Mexico, and Yucatan. From September to November 2021, we interviewed 88 key health professionals (HPs) (physicians, nurses, nutritionists, and others) from the PHC and 80 parents of children under 5 years old. In addition, nine focus groups were conducted with parents and caregivers. The data obtained were triangulated with information from focus groups and semi-structured interviews.

Results

Of the total interviews, 43.2% (n = 38) were nurses, 29.5% (n = 26) were physicians, 19.3% (n = 17) were nutritionists, and the rest were other health professionals. In the group of users, 97.6% (n = 121) were women. We identified contextual barriers, such as the lack of well-trained health professionals and the scarcest nutrition professionals, as material resources in the health units, without mentioning the low user attendance at their control consultations. Furthermore, we identified barriers related to the orientation and promotion of breastfeeding in health units, including a lack of specific strategies, ineffective communication, and the recommendations of commercial milk formulas.

Conclusion

The results presented reflect the reality of Mexico in relation to BF, making it urgent to take immediate action to improve the quality of nutritional care related to the promotion and orientation of BF at the PHC.
Current status of PSMA-targeted imaging and therapy
Authors: Wang Hui , Li GuanNan , Zhao Jie , Eiber Matthias , Tian Rong
Published: January 2024

Currently, the incidence of prostate cancer is increasing, and it has become a great threat to men’s health. The detection, staging, and follow-up of prostate cancer patients are inseparable from morphology or magnetic resonance imaging (MRI). However, these do not fully meet the needs of diagnosis and patient management. In particular, owing to the late diagnosis, metastatic castration-resistant prostate cancer (mCRPC) patients usually have poor survival and few options for further effective treatment. Prostate-specific membrane antigen (PSMA), because of its overexpression on prostate cancer cells, has gained interest due to its application in the imaging and theranostics field. Several PSMA radioligands have been developed for imaging and treating prostate cancer. Many clinical trials have assessed the efficacy and safety profiles of these radionuclide agents and show promise in patients who have exhausted other standard treatment options. To date, several small compounds for targeting PSMA have been developed, and 68Ga-PSMA-11 and 18F-DCFPyL have been approved by the United States (US) Food and Drug Administration (FDA) for imaging of prostate cancer. 111In- or 99mTc-labeled PSMA-ligand can guide surgeons searching for radioactive metastatic lymph nodes, and 177Lu- or 225Ac-labeled PSMA-ligand can be used for internal radiotherapy. Moreover, some molecules for therapeutic application are undergoing different stages of clinical trials. In this review, we present current perspectives on the use of PSMA-targeted imaging and theranostics in prostate cancer. As PSMA-targeted imaging and therapeutics are becoming the standard of care for prostate cancer patients, we emphasize the importance of integrating nuclear medicine physicians into multidisciplinary oncology teams.
Dimethyloxalylglycine pretreatment of living donor alleviates both donor and graft liver ischemia-reperfusion injury in rats
Authors: Jia Degong , Zhao Minjie , Luo Jiefu , Li Shengwei , Gong Jianping , Cheng Mingxiang
Published: January 2024

Background:Under the circumstance of the increasing waiting list for liver transplantation, living donor liver transplantation (LDLT) can alleviate the shortage of liver donors to some extent. However, how to reduce both donor and graft ischemia-reperfusion injury (IRI) is still an unsolved problem in LDLT. Hypoxia-induced transcription factor 1 (HIF1) activation is considered an important mechanism of cellular adaptation to hypoxia, and early activation of HIF1 may be a new way to alleviate liver IRI. Therefore, we aimed to investigate the impact of the HIF1 stabilizer dimethyloxalylglycine (DMOG) on IRI and the survival rate of donors and recipients of rat LDLT.

Methods:Seventy percent partial liver resection and 30% partial liver transplantation were used to simulate donor and recipient of clinical LDLT. Rats were treated with DMOG (40 mg/kg) or with an equivalent amount of saline. The expression of HIF1 and downstream targets was analyzed after 2 h of reperfusion. Liver function and histopathology, apoptosis and oxidative stress levels were detected 6 h after reperfusion. At the same time, the 7-day survival rate of rats was calculated.

Results:DMOG pretreatment significantly reduced IR-induced injury in the donor and recipient, which was manifested by reducing liver function damage and promoting tissue recovery. Meanwhile, compared with the untreated group, the oxidative stress level and the cell apoptosis rate were decreased in the group pretreated with DMOG. In addition, the transcription and expression of HIF1 target genes in the DMOG group were significantly enhanced. Remarkably, DMOG also increased the survival rate of the recipient.

Conclusion:This study provides the first evidence that DMOG pretreatment of donors significantly alleviates liver IRI in both donors and recipients and increases the survival rate of recipients in LDLT. Therefore, DMOG may be a promising strategy for improving LDLT in the future.
Potentially suitable habitats of Daodi goji berry in China under climate change
Authors: Li Jianling , Deng Changrong , Duan Guozhen , Wang Zhanlin , Zhang Yede , Fan Guanghui
Published: January 2024

Introduction

Goji berry (Lycium barbarum L.) is a famous edible and medicinal herb worldwide with considerable consumption. The recent cultivation of goji berries in the Daodi region was seriously reduced due to increased production costs and the influence of policy on preventing nongrain use of arable land in China. Consequently, production of Daodi goji berry was insufficient to meet market demands for high-quality medicinal materials. Searching for regions similar to the Daodi region was necessary.

Methods

The MaxEnt model was used to predicted the current and future potential regions suitable for goji berry in China based on the environmental characteristics of the Daodi region (including Zhongning County of Zhongwei prefecture-level city, and its surroundings), and the ArcGIS software was used to analyze the changes in its suitable region.

Results

The results showed that when the parameters were FC = LQHP and RM = 2.1, the MaxEnt model was optimal, and the AUC and TSS values were greater than 0.90. The mean temperature and precipitation of the coldest quarter were the most critical variables shaping the distribution of Daodi goji berries. Under current climate conditions, the suitable habitats of the Daodi goji berry were 45,973.88 km2, accounting for 0.48% of China’s land area, which were concentrated in the central and western Ningxia Province (22,589.42 km2), and the central region of Gansu Province (18,787.07 km2) bordering western Ningxia. Under future climate scenarios, the suitable area was higher than that under current climate conditions and reached the maximum under RCP 6.0 (91,256.42 km2) in the 2050s and RCP 8.5 (82,459.17 km2) in the 2070s. The expansion regions were mainly distributed in the northeast of the current suitable ranges, and the distributional centroids were mainly shifted to the northeast. The moderately and highly suitable overlapping habitats were mainly distributed in Baiyin (7,241.75 km2), Zhongwei (6,757.81 km2), and Wuzhong (5, 236.87 km2) prefecture-level cities.

Discussion

In this stduy, MaxEnt and ArcGIS were applied to predict and analyze the suitable habitats of Daodi goji berry in China under climate change. Our results indicate that climate warming is conducive to cultivating Daodi goji berry and will not cause a shift in the Daodi region. The goji berry produced in Baiyin could be used to satisfy the demand for high-quality medicinal materials. This study addresses the insufficient supply and guides the cultivation of Daodi goji berry.
The molecular structure, biological roles, and inhibition of plant pathogenic fungal chitin deacetylases
Authors: Mapuranga Johannes , Chang Jiaying , Li Hao , Zhang Yingdan , Li Ruolin , Song Lulu , Zhang Na , Yang Wenxiang
Published: January 2024

Chitin/polysaccharide deacetylases belong to the carbohydrate esterases family 4 (CE4 enzymes). They play a crucial role in modifying the physiochemical characteristics of structural polysaccharides and are also involved in a wide range of biological processes such as fungal autolysis, spore formation, cell wall formation and integrity, and germling adhesion. These enzymes are mostly common in fungi, marine bacteria, and a limited number of insects. They facilitate the deacetylation of chitin which is a structural biopolymer that is abundantly found in fungal cell walls and spores and also in the cuticle and peritrophic matrices of insects. The deacetylases exhibit specificity towards a substrate containing a sequence of four GlcNAc units, with one of these units being subjected to deacetylation. Chitin deacetylation results in the formation of chitosan, which is a poor substrate for host plant chitinases, therefore it can suppress the host immune response triggered by fungal pathogens and enhance pathogen virulence and colonization. This review discusses plant pathogenic fungal chitin/polysaccharide deacetylases including their structure, substrate specificity, biological roles and some recently discovered chitin deacetylase inhibitors that can help to mitigate plant fungal diseases. This review provides fundamental knowledge that will undoubtedly lead to the rational design of novel inhibitors that target pathogenic fungal chitin deacetylases, which will also aid in the management of plant diseases, thereby safeguarding global food security.
Green insurance, technology insurance, and corporate green innovation
Authors: Pu Chengyi , Mo Chou , Li Peihuan
Published: January 2024

The “dual-carbon” strategy was first written into China's “Government Work Report” in 2021, bringing green low-carbon development and green innovation to an unprecedented height. At the financial policy level, the Chinese government actively promotes the development of a green financial system represented by green bonds, green insurance, science and technology insurance, and green credit. Existing literature studies on green insurance and science and technology insurance mainly focus on the relevant legal system, the impact on corporate and risk management system, insurance types and service models, subsidy policies, and the problems and challenges they face. However, existing studies have studied the impact of green insurance and science and technology insurance on corporate' green innovation. In particular, the impact of their interaction on corporate' green innovation is rarely mentioned. From the perspective of green insurance and science and technology insurance to promote corporate' green innovation, based on the data sample of Shanghai and Shenzhen A-share listed companies from 2014 to 2015, this paper comprehensively investigated and analyzed the impact of green insurance and science and technology insurance on corporate' green innovation, and analyzed the interaction effect of the two. The research perspective of enterprise green innovation is expanded. The analysis conclusion of this paper provides new ideas and empirical evidence for Chinese corporate to realize faster and more efficient green transformation, which is conducive to stimulating the vitality of enterprise green innovation and development.
Using skeletal position to estimate human error rates in telemanipulator operators
Authors: Piercy Thomas , Herrmann Guido , Cangelosi Angelo , Zoulias Ioannis Dimitrios , Lopez Erwin
Published: January 2024

In current telerobotics and telemanipulator applications, operators must perform a wide variety of tasks, often with a high risk associated with failure. A system designed to generate data-based behavioural estimations using observed operator features could be used to reduce risks in industrial teleoperation. This paper describes a non-invasive bio-mechanical feature capture method for teleoperators used to trial novel human-error rate estimators which, in future work, are intended to improve operational safety by providing behavioural and postural feedback to the operator. Operator monitoring studies were conducted in situ using the MASCOT teleoperation system at UKAEA RACE; the operators were given controlled tasks to complete during observation. Building upon existing works for vehicle-driver intention estimation and robotic surgery operator analysis, we used 3D point-cloud data capture using a commercially available depth camera to estimate an operator’s skeletal pose. A total of 14 operators were observed and recorded for a total of approximately 8 h, each completing a baseline task and a task designed to induce detectable but safe collisions. Skeletal pose was estimated, collision statistics were recorded, and questionnaire-based psychological assessments were made, providing a database of qualitative and quantitative data. We then trialled data-driven analysis by using statistical and machine learning regression techniques (SVR) to estimate collision rates. We further perform and present an input variable sensitivity analysis for our selected features.
Novel calcaneal plate versus traditional philos plate for treating split fractures of humeral greater tuberosity
Authors: Wang Fei , Niu Xiaohui , Xia Haibo , Liang Wei , Hu Zhen , Lan Jun
Published: January 2024

Purpose

To compare the effect of two internal fixation methods of calcaneal plate fixation and Philos plate fixation in treating split fractures of humeral greater tuberosity.

Patients and methods

A total of 37 patients with split fractures of humeral greater tuberosity were retrospectively analyzed from September 2016 to April 2021. Enrolled patients were divided into Group A (calcaneal anatomical locking plates), and Group B [Proximal Humeral Internal Locking System (PHILOS)]. The demographics, injury-related variables, surgery-related variables, Constant-Murley score, and postoperative complication between the two groups were compared.

Results

There were 16 in Group A and 21 in Group B. Fracture union was achieved in all patients, with an average of 11.9 months. The mean length of incision was significantly different between groups (Group A, 6.16 cm ± 1.07 cm; Group B, 9.09 cm ± 1.30 cm, p < 0.05). Significant difference was observed by comparing bleeding loss between Group A and Group B (Group A, 45.41 ± 11.19 ml; Group B, 106.06 ± 11.12 ml, p < 0.05). After 6 months of treatment, the average Constant-Murley score of Group A was significantly higher than that of Group B (p < 0.05). In terms of operation, no significant difference was observed between groups. In addition, two patients had shoulder impingement syndrome in Group B, and none in Group A.

Conclusion

Calcaneal anatomical locking plate fixation is superior to Philos plate fixation in surgical trauma and bleeding loss. Our study provides an alternative technique for treating split fractures of humeral greater tuberosity.
Review: Salmonella Dublin in dairy cattle
Authors: Velasquez-Munoz Ana , Castro-Vargas Rafael , Cullens-Nobis Faith M. , Mani Rinosh , Abuelo Angel
Published: January 2024

Salmonella entericaserovar Dublin (S. Dublin) is a bacterium host-adapted to cattle with increasing prevalence in dairy facilities. It can severely affect cattle health, producing high morbidity and mortality in young calves and reducing the performance of mature animals.SalmonellaDublin is difficult to control and eradicate from herds, as it can be shed from clinically normal animals. In addition,S. Dublin is a zoonotic bacterium that can be lethal for humans and pose a risk for human and animal health due to its multi-drug resistant characteristics. This review provides an overview ofS. Dublin as a pathogen in dairy facilities, the risk factors associated with infection, and current strategies for preventing and controlling this disease. Furthermore, current gaps in knowledge are also discussed.
Experimental Study on Hypersonic Double-Wedge Induced Flow Based on Plasma Active Actuation Array
Authors: Yang Bo , Yang Hesen , Zhao Ning , Liang Hua , Su Zhi , Zhang Dongsheng
Published: January 2024

The double-wedge configuration is a typical characteristic shape of the rudder surface of high-speed aircraft. The impact of the shock wave/boundary layer interaction and the shock wave/shock wave interaction resulting from the double wedge on aircraft aerodynamics cannot be ignored. The aerodynamic performance of the aircraft would be seriously affected. Accordingly, to reduce the wave drag, and to relieve the thermal load and pressure load, flow control is required for the shock wave/shock wave interaction and the shock wave/boundary layer interaction induced by the double-wedge configuration. In this paper, double-wedge shock wave/shock wave interaction is controlled by a high-energy surface arc discharge array and observed by high-speed schlieren flow field measurement at Mach 8. The 30-channel discharge array is set on the primary wedge plane, and actuation is generated. Hypersonic V shock wave/shock wave interaction is effectively controlled by the shock wave array induced by the high-energy surface arc discharge array, which makes the shock wave/shock wave interaction structure disappear or intermittent. The potential control mechanism is to reduce strong shock wave interaction by transforming the type of shock wave interaction. Therefore, the ability of plasma array actuation to control complex shock wave/shock wave interaction is verified, which provides a new method for hypersonic shock wave/shock wave interaction control.
Study of Starting Performance of a Series Hybrid Aero Propulsion System
Authors: Zhu Jianfeng , Huang Guochen , Xu Maoguang , Liu Ming , Diao Bo , Li Po
Published: January 2024

Combined with the development trend of high speed generators and the high voltage of DC microgrids in high-power series hybrid aero propulsion system, a set of hybrid systems with a power of 200 kW, voltage of 540 V, and speed of 21,000 r/min is established in this article. Ground starting tests were conducted, focusing on the analysis of the coupling characteristics between the engine, generator and motors/propellers during the starting process, and further facilitated the optimization of the strategy of starting control. Firstly, the starting process of the series hybrid aero propulsion system mainly consists of four stages: the turboshaft engine is started, the gas turbine speed is increased, the controlled rectification intervenes, and the electric propeller is activated. The recommended definition of the idle state of the 200 kW hybrid propulsion system in this paper is as follows: power turbine speed NP = 10,500 rpm, grid system voltage UDC = 540 V, and the minimum stable power state of the electric motor PM = 150 W. Furthermore, experiments reveal that during the starting process, the resistance value and the rectification strategy, respectively, affect the steady-state and dynamic characteristics of the power turbine speed. By comparing multiple sets of experiments and utilizing data fitting software for optimal design, the results indicate that, based on the starting strategy of no-load protection and two-step controlled rectification, the total duration of the optimized starting process is shortened by 64.7%, and the gas turbine speed is reduced by 22.7% compared to the pre-optimized state. The starting control sequence is clearer, and the optimization effect is significant.
The Advancement and Prospects of the Tree Trunk Injection Technique in the Prevention and Control of Diseases and Pests
Authors: Shang Qingqing , Lu Hongcai , Yang Mengdi , Wu Yujie , Chen Qing
Published: January 2024

Traditional spraying of pesticides causes significant drift losses, and the residues of pesticides can also affect non-targeted organisms in the environment. Tree injection technology is a precise and targeted pesticide delivery method used in the prevention and treatment of tree and fruit tree pest infestations. It uses the tree’s xylem to transport the injected pesticides throughout the entire plant, reducing pesticide exposure in an open environment. This review summarizes the basic principles and development process of tree injection technology, compares its advantages with other application techniques, describes the development of injection equipment and key information to be aware of, and proposes suggestions for future research directions in injection application techniques.
The Influencing Factors Analysis of Aquaculture Mechanization Development in Liaoning, China
Authors: Yu Lixingbo , Wang Haiheng , Ren Anqi , Han Fengfan , Jia Fei , Hou Haochen , Liu Ying
Published: January 2024

Promoting the mechanization of aquaculture is one of the most important supporting measures to ensure the high-quality development of the aquaculture industry in China. In order to solve the problems of predominantly manual work and to decrease the costs of aquaculture, the influencing factors of China’s aquaculture mechanization were systematically analyzed. The triple bottom theory was selected, and three aspects were identified, including environmental, economic, and social aspects. Through the literature review, the Delphi method, and the analytic hierarchy process, the comprehensive evaluation indicator system, including 18 influencing factors, was proposed. Moreover, the fuzzy comprehensive evaluation method was combined with the model to solve the evaluation results. A case study in Liaoning Province was offered and, according to the analysis results, the economic aspect at the first level was the most critical factor; the financial subsidy for the purchase of aquaculture machinery, the energy consumption of the machinery and equipment, and the promotion and use of aquaculture technology were the most important factors and had the greatest impact on the development of aquaculture mechanization in China. The effective implementation paths and countermeasures were proposed, such as the promotion of mechanized equipment and the enhancement of the machinery purchase subsidies, in order to provide an important decision-making basis for the improvement of the level of aquaculture mechanization.
Unveiling the Relationship between Ceftobiprole and High-Molecular-Mass (HMM) Penicillin-Binding Proteins (PBPs) in Enterococcus faecalis
Authors: Conti Paola , Lazzaro Lorenzo Mattia , Longo Fabio , Lenzo Federica , Giardina Alessandra , Fortuna Sebastiano Alberto , Stefani Stefania , Campanile Floriana
Published: January 2024

Low-affinity PBP4, historically linked to penicillin resistance in Enterococcus faecalis, may still have affinity for novel cephalosporins. Ceftobiprole (BPR) is a common therapeutic choice, even with PBP4-related overexpression and amino acid substitution due to mutations. Our study aims to explore the interaction between BPR and High-Molecular-Mass (HMM) low-reactive PBPs in Penicillin-Resistant-Ampicillin-Susceptible/Ceftobiprole Non-Susceptible (PRAS/BPR-NS) E. faecalis clinical isolates. We conducted competition assays examining class A and B HMM PBPs from four PRAS/BPR-NS E. faecalis strains using purified membrane proteins and fluorescent penicillin (Bocillin FL), in treated and untreated conditions. Interaction strength was assessed calculating the 50% inhibitory concentration (IC50) values for ceftobiprole, by analyzing fluorescence intensity trends. Due to its low affinity, PBP4 did not display significant acylation among all strains. Moreover, both PBP1a and PBP1b showed a similar insensitivity trend. Conversely, other PBPs showed IC50 values ranging from 1/2-fold to 4-fold MICs. Upon higher BPR concentrations, increased percentages of PBP4 inhibition were observed in all strains. Our results support the hypothesis that PBP4 is necessary but not sufficient for BPR resistance, changing the paradigm for enterococcal cephalosporin resistance. We hypothesize that cooperation between class B PBP4 and at least one bifunctional class A PBP could be required to synthesize peptidoglycan and promote growth.
Antimicrobial Resistance in Streptococcus pneumoniae before and after the Introduction of Pneumococcal Conjugate Vaccines in Brazil: A Systematic Review
Authors: Knupp-Pereira Patricia Alice , Cabral Amanda Seabra , Dolores Ítalo Moraes , da Silva Amanda Beiral , Póvoa Helvécio Cardoso Correa , Neves Felipe Piedade Gonçalves
Published: January 2024

Streptococcus pneumoniae causes serious illnesses, such as pneumonia, bacteremia, and meningitis, mainly in immunocompromised individuals and those of extreme ages. Currently, pneumococcal conjugate vaccines (PCVs) are the best allies against pneumococcal diseases. In Brazil, the 10-valent and 13-valent PCVs have been available since 2010, but the threat of antimicrobial resistance persists and has been changing over time. We conducted a systematic review of the literature with works published since 2000, generating a parallel between susceptibility data on isolates recovered from colonization and invasive diseases before and after the implementation of PCVs for routine childhood use in Brazil. This systematic review was based on the Cochrane Handbook for Systematic Reviews of Interventions and Preferred Reporting Items for Systematic Literature Reviews and Meta-Analyses (PRISMA) guidelines. Despite the inclusion of PCVs at a large scale in the national territory, high frequencies of non-susceptibility to important drugs used in pneumococcal diseases are still observed, especially penicillin, as well as increasing resistance to macrolides. However, there are still drugs for which pneumococci have a comprehensive sensitivity profile.
Pathological Gait Classification Using Early and Late Fusion of Foot Pressure and Skeleton Data
Authors: Naseem Muhammad Tahir , Seo Haneol , Kim Na-Hyun , Lee Chan-Su
Published: January 2024

Classifying pathological gaits is crucial for identifying impairments in specific areas of the human body. Previous studies have extensively employed machine learning and deep learning (DL) methods, using various wearable (e.g., inertial sensors) and non-wearable (e.g., foot pressure plates and depth cameras) sensors. This study proposes early and late fusion methods through DL to categorize one normal and five abnormal (antalgic, lurch, steppage, stiff-legged, and Trendelenburg) pathological gaits. Initially, single-modal approaches were utilized: first, foot pressure data were augmented for transformer-based models; second, skeleton data were applied to a spatiotemporal graph convolutional network (ST-GCN). Subsequently, a multi-modal approach using early fusion by concatenating features from both the foot pressure and skeleton datasets was introduced. Finally, multi-modal fusions, applying early fusion to the feature vector and late fusion by merging outputs from both modalities with and without varying weights, were evaluated. The foot pressure-based and skeleton-based models achieved 99.04% and 78.24% accuracy, respectively. The proposed multi-modal approach using early fusion achieved 99.86% accuracy, whereas the late fusion method achieved 96.95% accuracy without weights and 99.17% accuracy with different weights. Thus, the proposed multi-modal models using early fusion methods demonstrated state-of-the-art performance on the GIST pathological gait database.
The Validity and Absolute Reliability of Lower Extremity Angle Values on Full-Leg Standing Radiographs Using the TraumaMeter Software
Authors: León-Muñoz Vicente J. , Hurtado-Avilés José , Moya-Angeler Joaquín , Valero-Cifuentes Gregorio , Hernández-Martínez Irene , Castillo-Botero Alejandro J. , Lante Erica , Martínez-Sola Rocío , Santonja-Renedo Fernando , Sánchez-Martínez Francisco J. , Ferrer-López Vicente , Salmerón-Martínez Emilio José , Santonja-Medina Fernando
Published: January 2024

To establish classifications and to obtain pre- and post-operative information on patient-specific alignments, it is necessary to measure different angular values accurately and precisely, mainly on weight-bearing, full-length anteroposterior X-rays of the lower limbs (LLRs). This study evaluated angular measurements’ validity and absolute reliability on LLRs with a self-developed, computer-aided measurement system (TraumaMeter v.873). Eight independent observers measured the preoperative mechanical hip-knee-ankle (mHKA) angle of 52 lower extremities (26 cases) in a blinded fashion on three occasions separated by two weeks. We obtained an intra-observer mean bias error (MBE) of 0.40°, a standard deviation (SD) of 0.11°, and a 95% confidence interval (CI) of 0.37°–0.43°. We also obtained an inter-observer MBE of 0.49°, an SD of 0.15°, and a 95% C of 0.45°–0.53°. The intra-observer MBE for the measurement pair between the second and the first measurement round (T2T1) was 0.43°, the SD was 0.13°, and the 95% CI was 0.39°–0.47°; the MBE between the third and the second round (T3T2) was 0.37°, with an SD of 0.10° and a 95% CI of 0.34°–0.40°; and the MBE between the third and the first round (T3T1) was 0.40°, with an SD of 0.10° and a 95% CI of 0.37°–0.43°. The interobserver MBE for the first round of measurements was 0.52°, with an SD of 0.16° and a 95% CI of 0.48°–0.56°; the MBE for the second round was 0.50°, with an SD of 0.15° and a 95% CI of 0.46°–0.54°; and the MBE for the third round was 0.46°, with an SD of 0.14° and a 95% CI of 0.42°–0.50°. There were no statistically significant differences in the inter-observer errors for the three tests. In the case of the intra-observer analysis, there were differences between T2T1 and between T3T2, but these differences were minimal, with no overlaps in the lower or upper values, respectively, of the confidence intervals. These results led us to conclude that the TraumaMeter v.873 software extension for measuring lower-limb angles in LLRs is an accurate tool with low intra- and inter-observer variability.
Insights into Genetic and Physiological Characteristics of Clover Rhizobia in Afghanistan Soils
Authors: Habibi Safiullah , Yasuda Michiko , Aryan Shafiqullah , Yokoyama Tadashi , Saighani Kalimullah , Ohkama-Ohtsu Naoko
Published: January 2024

Livestock production in Afghanistan highly relies on grazing and clover feed, which is a key component of pastures and forage crops. This study elucidated the genetic diversity of clover-nodulating rhizobia in different ecological regions and their effects on clover growth. A total of 57 rhizobia were isolated and their genetic diversities were studied through 16S rRNA and nifD genes. The isolates were inoculated to clover (Afghan local variety), to investigate the potential of nitrogen fixation and influences of clover growth. The 16S rRNA gene analysis showed two distinct groups of Rhizobium (94.7%) and Ensifer (5.3%) species. The nifD phylogenetic relationship revealed a high similarity to Rhizobium and a novel lineage group close to Rhizobium leguminosarum species. In the plant test, different genotypes significantly (p < 0.01) exhibited an increase in plant biomass production, compared to the un-inoculated plants. Among genotypes, the highest plant biomass was recorded in PC8 (1769.0 mg/plant) and PC9 (1409.2 mg/plant) isolates as compared to un-inoculated plants (144.0 mg/plant). Moreover, these isolates showed maximum nitrogen fixation rates of 8.2 and 6.5 µM/plant, respectively. These isolates were identified as the most promising rhizobial strains for developing biofertilizers in the context of Afghanistan.
An Industrial Perspective and Intellectual Property Landscape on Solid-State Battery Technology with a Focus on Solid-State Electrolyte Chemistries
Authors: Karkar Zouina , Houache Mohamed S. E. , Yim Chae-Ho , Abu-Lebdeh Yaser
Published: January 2024

This review focuses on the promising technology of solid-state batteries (SSBs) that utilize lithium metal and solid electrolytes. SSBs offer significant advantages in terms of high energy density and enhanced safety. This review categorizes solid electrolytes into four classes: polymer, oxide, hybrid, and sulfide solid electrolytes. Each class has its own unique characteristics and benefits. By exploring these different classes, this review aims to shed light on the diversity of materials and their contributions to the advancement of SSB technology. In order to gain insights into the latest technological developments and identify potential avenues for accelerating the progress of SSBs, this review examines the intellectual property landscape related to solid electrolytes. Thus, this review focuses on the recent SSB technology patent filed by the main companies in this area, chosen based on their contribution and influence in the field of batteries. The analysis of the patent application was performed through the Espacenet database. The number of patents related to SSBs from Toyota, Samsung, and LG is very important; they represent more than 3400 patents, the equivalent of 2/3 of the world’s patent production in the field of SSBs. In addition to focusing on these three famous companies, we also focused on 15 other companies by analyzing a hundred patents. The objective of this review is to provide a comprehensive overview of the strategies employed by various companies in the field of solid-state battery technologies, bridging the gap between applied and academic research. Some of the technologies presented in this review have already been commercialized and, certainly, an acceleration in SSB industrialization will be seen in the years to come.