bluuebunny/crossref_metadata_2025 huggingface dataset:
Green Balanced Scorecard: A Tool of Sustainable Information Systems for an Energy Efficient Business
Authors: Stavropoulou Eirini , Spinthiropoulos Konstantinos , Ragazou Konstantina , Papademetriou Christos , Passas Ioannis
Published: September 2023

A green balanced scorecard allows businesses to monitor their environmental progress and energy use, as well as quantify their achievements toward decreasing their ecological footprint. The scope of the current research is twofold: (i) to highlight the new sustainable profile of the Balanced Scorecard; and (ii) to investigate the contribution of the Sustainable Balanced Scorecard to the achievement of the energy efficiency and environmental goals of businesses. The present research study applies the rigorous bibliometric analysis technique and the PRISMA method for the years 2011–2023 to investigate the most popular research sources and documents, where the most frequently used keywords and research trends are utilized to answer the research question. In addition, findings from the network, content, and cluster analyses were visualized with the use of the bibliometric tools of Biblioshiny and VOSviewer. The results of the study indicate that businesses have made it a priority to build an energy-efficient management system based on the Sustainable Balanced Scorecard, which will help them achieve their organization’s environmental strategic goals. Moreover, a new era in the Sustainable Balanced Scorecard, that of the Contact Balanced Scorecard, has started emerging in the field. This article explains how the Sustainable Balanced Scorecard benefits organizations by enabling them to enhance their performance on both the economic and social fronts as well as the environmental front. Finally, by employing strategic evaluation methods like the Sustainable Balanced Scorecard to analyze organizations’ sustainability performance, the present study effort supports the sustainable growth of enterprises.
Switching Operations in 110 kV Networks in the Context of Synchro-Check and Mitigation of Switching Stress by Utilizing Proper Control of Renewables and Energy Storages
Authors: Łowczowski Krzysztof , Miller Piotr , Udzik Magdalena , Złotecka Daria
Published: September 2023

Synchro-check (SC) relays are devices that control synchronization between different fragments of power system networks. Synchronism is monitored by controlling the voltage amplitude, angle, and frequency differences. The goal of monitoring is to avoid dangerous transients after the circuit breaker (CB) closing, which occur when the differences between the voltage, angle, and frequency at both sides of the CB are above the threshold. Although the idea of SC is straightforward, one needs to realize that blocking of the CB closing may cause even more severe issues, i.e., the deterioration of power system stability and safety. The conflicting goals of the switching operation result in many different interpretations, regarding the recommended settings and utilization of the SC relays in different locations of power system networks. The paper presents considerations connected with switching operations in power systems, in particular, in 110 kV networks. Different problems connected with switching operations are highlighted and described. Moreover, the switching-relevant parameters and switching constraints are provided. Further, the simulation results connected with switching operations are presented, the realistic possible voltage and angle differences before switching operations are provided, and the impacts of mitigation measures—renewables and energy storage—are estimated. Finally, the proposed procedures and algorithms of switching stress mitigation are provided.
Sex-Related Differences of Ginkgo biloba in Growth Traits and Wood Properties
Authors: Li Jiaqi , Su Xiandi , Guo Jing , Xu Wei , Feng Lei , Wang Tongli , Fu Fangfang , Wang Guibin
Published: September 2023

Ginkgo biloba is one of the most widely cultivated dioecious timber trees in China. Understanding sex-related differences and how they affect growth traits and wood properties is crucial for informed management and optimal utilization of ginkgoes. In the present study, we collected 42 ginkgo samples and conducted DNA molecular identification to determine their sex. The result was a 1:1 ratio of male to female specimens. In addition, we measured 16 growth-trait and wood-property indices for these samples using advanced equipment, such as X-ray diffraction (XRD) and the Hitman ST300 standing tree tool. For growth traits, significant differences were observed between male and female ginkgoes in terms of the diameter at breast height (DBH), clear bole height (CBH), height, and volume. Significant differences were identified in wood properties between male and female ginkgoes in terms of the degree of cellulose crystallinity (DCC), cell length, cell wall thickness, and wall-to-lumen ratio. Tracheids from female trees were found to be wider, with thicker cell walls, than those from male trees. Principal component analysis (PCA) showed that there was a slight separation between the sexes in terms of all growth traits, whereas there was no separation in wood properties. The membership function value (MFV) also showed that male ginkgo exhibited a more robust phenotype than female ginkgo. The selection of male ginkgo for breeding and utilization offers distinct advantages for practical production.
Systematic Review of Research on Reality Technology-Based Forest Education
Authors: Choi Seonhye , Ha Siyeon , Choe Jiwon
Published: September 2023

This study aimed to analyze the effectiveness of reality technology programs in forest education by systematically reviewing prior studies that have verified the effectiveness of reality technology-aided forest education content. Additionally, we checked the current status of reality technology content-based forest-related education. We searched for data on forest education using reality technology published until November 2022 in both domestic and foreign web-based academic databases. In total, 117 research papers were identified, and 13 were selected based on the data selection criteria. After systematic analysis, we inferred the following: First, most reality technology-based forest education programs use augmented reality (AR); the software is customized and developed for mobile devices because AR is effective in two-way communication owing to the nature of the technology. Second, forest education showed greater cognitive and affective effects when reality technology was used (cognitive effect: 71.4%; affective effect: 63%) than when it was not used. Third, forest education using reality technology produced more than 90% cognitive and affective effects (cognitive effect: 90%; affective effect: 100%). Therefore, forest education using realistic technology can have a positive effect indoors. Although these results are difficult to generalize, they can be used as basic data for future research on reality technology-based forest education.
Labeling and Tracking of Individual Human Mesenchymal Stromal Cells Using Photoconvertible Fluorescent Microcapsules
Authors: Sindeeva Olga A. , Demina Polina A. , Kozyreva Zhanna V. , Muslimov Albert R. , Gusliakova Olga I. , Laushkina Valeriia O. , Mordovina Ekaterina A. , Tsyupka Daria , Epifanovskaya Olga S. , Sapach Anastasiia Yu. , Goryacheva Irina Yu. , Sukhorukov Gleb B.
Published: September 2023

The behavior and migration of human mesenchymal stromal cells (hMSCs) are focal points of research in the biomedical field. One of the major aspects is potential therapy using hMCS, but at present, the safety of their use is still controversial owing to limited data on changes that occur with hMSCs in the long term. Fluorescent photoconvertible proteins are intensively used today as “gold standard” to mark the individual cells and study single-cell interactions, migration processes, and the formation of pure lines. A crucial disadvantage of this method is the need for genetic modification of the primary culture, which casts doubt on the possibility of exploring the resulting clones in personalized medicine. Here we present a new approach for labeling and tracking hMSCs without genetic modification based on the application of cell-internalizable photoconvertible polyelectrolyte microcapsules (size: 2.6 ± 0.5 μm). These capsules were loaded with rhodamine B, and after thermal treatment, exhibited fluorescent photoconversion properties. Photoconvertible capsules demonstrated low cytotoxicity, did not affect the immunophenotype of the hMSCs, and maintained a high level of fluorescent signal for at least seven days. The developed approach was tested for cell tracking for four days and made it possible to trace the destiny of daughter cells without the need for additional labeling.
Specific Targeting of STAT3 in B Cells Suppresses Progression of B Cell Lymphoma
Authors: Wang Lipei , Zhou Mingqian , Kong Xiangyu , Wu Shouzhen , Ding Chuanlin , Hu Xiaoling , Guo Haixun , Yan Jun
Published: September 2023

The signal transducer and activator of transcription 3 (STAT3), which regulates multiple oncogenic processes, has been found to be constitutively activated in lymphoma, suggesting its potential as a therapeutic target. Here, we constructed an anti-CD19-N-(4-carboxycyclohexylmethyl) maleimide N-hydroxysuccinimide ester (SMCC)-protamine (CSP)-STAT3 small interfering RNA (siRNA) conjugate and demonstrated that the CSP-STAT3 siRNA conjugate could specifically bind to normal B cells and A20 lymphoma cells in vitro. It decreased the STAT3 expression in B cell lymphoma cell lines (A20, SU-DHL-2 and OCI-Ly3), resulting in reduced proliferation of lymphoma cells featured with lower S-phase and higher apoptosis. Using an A20 transplantable lymphoma model, we found that the CSP-STAT3 siRNA conjugate significantly inhibited tumor growth and weight. Ki-67, p-STAT3, STAT3, and serum IL-6 levels were all significantly reduced in A20-bearing mice treated with CSP-STAT3 siRNA. These findings indicate that specifically targeting STAT3 siRNA to B cell lymphoma cell lines can significantly decrease STAT3 activity and inhibit tumor progression in vitro and in vivo, suggesting its potential utilization for cancer treatment.
Targeting Key Players of Neuroendocrine Differentiation in Prostate Cancer
Authors: Zamora Irene , Freeman Michael R. , Encío Ignacio J. , Rotinen Mirja
Published: September 2023

Neuroendocrine prostate cancer (NEPC) is a highly aggressive subtype of prostate cancer (PC) that commonly emerges through a transdifferentiation process from prostate adenocarcinoma and evades conventional therapies. Extensive molecular research has revealed factors that drive lineage plasticity, uncovering novel therapeutic targets to be explored. A diverse array of targeting agents is currently under evaluation in pre-clinical and clinical studies with promising results in suppressing or reversing the neuroendocrine phenotype and inhibiting tumor growth and metastasis. This new knowledge has the potential to contribute to the development of novel therapeutic approaches that may enhance the clinical management and prognosis of this lethal disease. In the present review, we discuss molecular players involved in the neuroendocrine phenotype, and we explore therapeutic strategies that are currently under investigation for NEPC.
Isothiocyanates as Tubulin Polymerization Inhibitors—Synthesis and Structure–Activity Relationship Studies
Authors: Grzywa Renata , Psurski Mateusz , Gajda Anna , Gajda Tadeusz , Janczewski Łukasz
Published: September 2023

Among the various substances that interfere with the microtubule formation process, isothiocyanates (ITCs) are the group of compounds for which the binding mode and mechanism of action have not yet been explained. To better understand the structure–activity relationship of tubulin-isothiocyanate interactions, we designed and synthesized a series of sixteen known and novel, structurally diverse ITCs, including amino acid ester-derived isothiocyanates, bis-isothiocyanates, analogs of benzyl isothiocyanate, and phosphorus analogs of sulforaphane. All synthesized compounds and selected natural isothiocyanates (BITC, PEITC, AITC, and SFN) were tested in vitro to evaluate their antiproliferative activity, tubulin polymerization inhibition potential, and influence on cell cycle progression. The antiproliferative activity of most of the newly tested compounds exceeded the action of natural isothiocyanates, with four structures being more potent as tubulin polymerization inhibitors than BITC. As a confirmation of anti-tubulin activity, the correlation between polymerization inhibition and cell cycle arrest in the G2/M phase was observed for the most active compounds. In light of the biological results indicating significant differences in the impact of structurally diverse isothiocyanate on tubulin polymerization, in silico analysis was conducted to analyze the possible mode of isothiocyanate-tubulin binding and to show how it can influence the polymerization reaction.
Synthesis, Characterization, and Bioactivity of Mesoporous Bioactive Glass Codoped with Zinc and Silver
Authors: Yang Tsung-Ying , Chern Guann-In , Wang Wei-Hsun , Shih Chi-Jen
Published: September 2023

Due to the overconsumption of antimicrobials, antibiotic-resistant bacteria have become a critical health issue worldwide, especially methicillin-resistant S. aureus (MRSA) and vancomycin-resistant E. faecalis (VRE). Recently, many efforts have been made to load metals into bioactive glasses to enhance the multifunctionality of materials, such as antibacterial and osteoinductive functions. Zinc has been documented to stimulate the gene expression of various regulatory factors in bone cells. Meanwhile, previous studies have reported that silver and zinc could be a promising antibacterial combination with synergistic antimicrobial effects. Here, we sought to develop a biomaterial coreleasing zinc and silver, designated 80S-ZnAg, and to evaluate its antibacterial activity and biocompatibility. The textural analyses demonstrated different coreleasing patterns of zinc and silver for the materials. The chemical characterization revealed that the zinc in 80S-ZnAg could be the network modifier when its molar ratio was high, releasing more zinc; zinc could also be the network former when its molar ratio was low, showing an extremely low rate of release. However, the ICP results for 80S-Zn3Ag2 demonstrated up to 7.5 ppm of zinc and 67.6 ppm of silver. Among all the 80S-ZnAg materials, 80S-Zn3Ag2 demonstrated more marked antibacterial activity against MRSA and VRE than the others, with inhibition zones of 11.5 and 13.4 mm, respectively. The cytotoxicity assay exhibited nearly 90% cell viability at 20 mg/mL of 80-Zn3Ag2. Further clinical study is needed to develop an innovative biomaterial to address the issue of antibiotic resistance.
The Transcription Factor CsgD Contributes to Engineered Escherichia coli Resistance by Regulating Biofilm Formation and Stress Responses
Authors: Yan Cheng-Hai , Chen Fang-Hui , Yang Yu-Lu , Zhan Yu-Fan , Herman Richard A. , Gong Lu-Chan , Sheng Sheng , Wang Jun
Published: September 2023

The high cell density, immobilization and stability of biofilms are ideal characteristics for bacteria in resisting antibiotic therapy. CsgD is a transcription activating factor that regulates the synthesis of curly fimbriae and cellulose in Escherichia coli, thereby enhancing bacterial adhesion and promoting biofilm formation. To investigate the role of CsgD in biofilm formation and stress resistance in bacteria, the csgD deletion mutant ΔcsgD was successfully constructed from the engineered strain E. coli BL21(DE3) using the CRISPR/Cas9 gene-editing system. The results demonstrated that the biofilm of ΔcsgD decreased by 70.07% (p < 0.05). Additionally, the mobility and adhesion of ΔcsgD were inhibited due to the decrease in curly fimbriae and extracellular polymeric substances. Furthermore, ΔcsgD exhibited a significantly decreased resistance to acid, alkali and osmotic stress conditions (p < 0.05). RNA-Seq results revealed 491 differentially expressed genes between the parent strain and ΔcsgD, with enrichment primarily observed in metabolism-related processes as well as cell membrane structure and catalytic activity categories. Moreover, CsgD influenced the expression of biofilm and stress response genes pgaA, motB, fimA, fimC, iraP, ompA, osmC, sufE and elaB, indicating that the CsgD participated in the resistance of E. coli by regulating the expression of biofilm and stress response. In brief, the transcription factor CsgD plays a key role in the stress resistance of E. coli, and is a potential target for treating and controlling biofilm.
APPA Increases Lifespan and Stress Resistance via Lipid Metabolism and Insulin/IGF-1 Signal Pathway in Caenorhabditis elegans
Authors: Wang Shiyao , Lin Dongfa , Cao Jiaofei , Wang Liping
Published: September 2023

Animal studies have proven that 1-acetyl-5-phenyl-1H-pyrrol-3-yl acetate (APPA) is a powerful antioxidant as a novel aldose reductase inhibitor independently synthesized by our laboratory; however, there is no current information on APPA’s anti-aging mechanism. Therefore, this study examined the impact and mechanism of APPA’s anti-aging and anti-oxidation capacity using the Caenorhabditis elegans model. The results demonstrated that APPA increases C. elegans’ longevity without affecting the typical metabolism of Escherichia coli OP50 (OP50). APPA also had a non-toxic effect on C. elegans, increased locomotor ability, decreased the levels of reactive oxygen species, lipofuscin, and fat, and increased anti-stress capacity. QRT-PCR analysis further revealed that APPA upregulated the expression of antioxidant genes, including sod-3, gst-4, and hsp-16.2, and the critical downstream transcription factors, daf-16, skn-1, and hsf-1 of the insulin/insulin-like growth factor (IGF) receptor, daf-2. In addition, fat-6 and nhr-80 were upregulated. However, the APPA’s life-prolonging effects were absent on the daf-2, daf-16, skn-1, and hsf-1 mutants implying that the APPA’s life-prolonging mechanism depends on the insulin/IGF-1 signaling system. The transcriptome sequencing also revealed that the mitochondrial route was also strongly associated with the APPA life extension, consistent with mev-1 and isp-1 mutant life assays. These findings aid in the investigation of APPA’s longevity extension mechanism.
Ion fluxes Involved in the Adaptation of the Estuarine Diatom Coscinodiscus centralis Ehrenberg to Salinity Stress
Authors: Chen Changping , Hu Xiao , Gao Yahui , Liang Junrong , Sun Lin
Published: September 2023

Although estuarine diatoms have a wide range of salt tolerance, they are often severely stressed by elevated salt concentrations. It remains poorly understood how estuarine diatoms maintain ionic homeostasis under high-salinity conditions. Using a scanning ion-selective electrode technique, this study determined the fluxes of H+, Na+, and K+ involved in the acclimatization of the estuarine diatom Coscinodiscus centralis Ehrenberg after an elevation in salinity from 15 psu to 35 psu. The C. centralis cells exhibited marked H+ effluxes after a transient treatment (TT, 30 min) and short-term treatment (ST, 24 h). However, a drastic shift of H+ efflux toward an influx was induced in the long-term treatment (LT, 10 days). The Na+ flux under TT, ST, and LT salinity conditions was found to accelerate the Na+ efflux. More pronounced effects were observed under the ST and LT salinity conditions compared to the TT salinity condition. The K+ influx showed a significant increase under the LT salinity condition. However, the salinity-induced Na+/H+ exchange in the estuarine diatom was inhibited by amiloride and sodium orthovanadate. These results indicate that the Na+ extrusion in salt-stressed cells is mainly the result of an active Na+/H+ antiport across the plasma membrane. The pattern of ion fluxes under the TT and ST salinity conditions were different from those under the LT salinity conditions, suggesting an incomplete regulation of the acclimation process in the estuarine diatom under short-term salinity stress.
High Tumor-Infiltrating Lymphocyte Count Is Associated with Distinct Gene Expression Profile and Longer Patient Survival in Advanced Ovarian Cancer
Authors: Barna Andras Jozsef , Herold Zoltan , Acs Miklos , Bazsa Sandor , Gajdacsi Jozsef , Garay Tamas Marton , Herold Magdolna , Madaras Lilla , Muhl Dorottya , Nagy Akos , Szasz Attila Marcell , Dank Magdolna
Published: September 2023

Cancer-related immunity plays a significant role in the outcome of ovarian cancer, but the exact mechanisms are not fully explored. A retrospective, real-life observational study was conducted including 57 advanced ovarian cancer patients. Immunohistochemistry for CD4+, CD8+, and CD45+ was used for assessing tumor-infiltrating immune cells. Furthermore, an immune-related gene expression assay was performed on 12–10 samples from patients with less than and more than 1-year overall survival (OS), respectively. A higher number of CD4+ (p = 0.0028) and CD45+ (p = 0.0221) immune cells within the tumor microenvironment were associated with longer OS of patients. In a multivariate setting, higher CD4+ T cell infiltration predicted longer OS (p = 0.0392). Twenty-three differentially expressed genes—involved in antigen presentation, costimulatory signaling, matrix remodeling, metastasis formation, and myeloid cell activity—were found when comparing the prognostic groups. It was found that tumor-infiltrating immune cell counts are associated with peculiar gene expression patterns and bear prognostic information in ovarian cancer. SOX11 expression emerged and was validated as a predictive marker for OS.
The Benefits of Water from Nitrodi’s Spring: The In Vitro Studies Leading the Potential Clinical Applications
Authors: Mormile Ilaria , Tuccillo Fabiana , Della Casa Francesca , D’Aiuto Valentina , Montuori Nunzia , De Rosa Marina , Napolitano Filomena , de Paulis Amato , Rossi Francesca Wanda
Published: September 2023

Natural products (water, plants, and minerals) have been studied for diverse applications in health and disease. Since there has been a growing interest in the introduction of thermal water as a clinical complementary approach in the treatment of low-grade inflammation and stress-related conditions, this review focuses on the oldest spa in the world: Nitrodi’s spring. Substantial studies in the 1960s showed that both the internal and external use of Nitrodi’s water yielded several benefits in physiological processes and in treating certain disorders, mainly allergic and autoimmune inflammatory conditions. More recently, a novel interest in Nitrodi’s water has prompted researchers to further explore the effects of this water and shed light on the molecular mechanisms sustaining its therapeutic efficacy. In different epithelial cell models, Nitrodi’s water had strong promotional effects on proliferation, cell migration, cell viability, and fibroblast to myofibroblast transition, all of which essential for wound healing and tissue remodeling. Moreover, Nitrodi’s water exhibited anti-oxidant and anti-inflammatory properties through the inhibition of ROS production and protein S-nitrosylation. Here, we have collected the clinical and basic data on Nitrodi’s water and reviewed articles that have discussed its use as a potential treatment for several inflammatory and autoimmune diseases and age-related skin deterioration.
Unraveling the Kinetics of the 10–23 RNA-Cleaving DNAzyme
Authors: Montserrat Pagès Aida , Hertog Maarten , Nicolaï Bart , Spasic Dragana , Lammertyn Jeroen
Published: September 2023

DNA-based enzymes, or DNAzymes, are single-stranded DNA sequences with the ability to catalyze various chemical reactions, including the cleavage of the bond between two RNA nucleotides. Lately, an increasing interest has been observed in these RNA-cleaving DNAzymes in the biosensing and therapeutic fields for signal generation and the modulation of gene expression, respectively. Additionally, multiple efforts have been made to study the effects of the reaction environment and the sequence of the catalytic core on the conversion of the substrate into product. However, most of these studies have only reported alterations of the general reaction course, but only a few have focused on how each individual reaction step is affected. In this work, we present for the first time a mathematical model that describes and predicts the reaction of the 10–23 RNA-cleaving DNAzyme. Furthermore, the model has been employed to study the effect of temperature, magnesium cations and shorter substrate-binding arms of the DNAzyme on the different kinetic rate constants, broadening the range of conditions in which the model can be exploited. In conclusion, this work depicts the prospects of such mathematical models to study and anticipate the course of a reaction given a particular environment.
Three/Four-Dimensional Printed PLA Nano/Microstructures: Crystallization Principles and Practical Applications
Authors: Zhou Yufeng , Chen Jingbo , Liu Xuying , Xu Jianwei
Published: September 2023

Compared to traditional methods, three/four-dimensional (3D/4D) printing technologies allow rapid prototyping and mass customization, which are ideal for preparing nano/microstructures of soft polymer materials. Poly (lactic acid) (PLA) is a biopolymer material widely used in additive manufacturing (AM) because of its biocompatibility and biodegradability. Unfortunately, owing to its intrinsically poor nucleation ability, a PLA product is usually in an amorphous state after industrial processing, leading to some undesirable properties such as a barrier property and low thermal resistance. Crystallization mediation offers a most practical way to improve the properties of PLA products. Herein, we summarize and discuss 3D/4D printing technologies in the processing of PLA nano/microstructures, focusing on crystallization principles and practical applications including bio-inspired structures, flexible electronics and biomedical engineering mainly reported in the last five years. Moreover, the challenges and prospects of 3D/4D printing technologies in the fabrication of high-performance PLA materials nano/microstructures will also be discussed.
Anti-Rheumatoid Arthritis Pharmacodynamic Substances Screening of Periploca forrestii Schltr.: Component Analyses In Vitro and In Vivo Combined with Multi-Technical Metabolomics
Authors: Sun Jia , Zhou Zuying , Zhou Yang , Liu Ting , Li Yueting , Gong Zipeng , Jin Yang , Zheng Lin , Huang Yong
Published: September 2023

The purpose of this study was to elucidate the metabolic action patterns of P. forrestii against rheumatoid arthritis (RA) using metabolomics, and to obtain its potential effective substances for treating RA. First, the therapeutic effects of P. forrestii against RA were confirmed; second, the chemical composition of P. forrestii was analyzed, and 17 prototypes were absorbed into blood; subsequently, plasma metabolomics studies using UPLC-Triple-TOF-MS/MS and GC-MS were performed to disclose the metabolomics alterations in groups, which revealed 38 altered metabolites after drug intervention. These metabolites were all associated with the arthritis pathophysiology process (−log(p) > 1.6). Among them, sorted by variable important in projection (VIP), the metabolites affected (VIP ≥ 1.72) belonged to lipid metabolites. Finally, Pearson’s analysis between endogenous metabolites and exogenous compounds was conducted to obtain potential pharmacological substances for the P. forrestii treatment of RA, which showed a high correlation between five blood-absorbed components and P. forrestii-regulated metabolites. This information provides a basis for the selection of metabolic action modes for P. forrestii clinical application dosage, and potential pharmacological substances that exerted anti-RA effects of P. forrestii were discovered. The study provided an experimental basis for further research on pharmacoequivalence, molecular mechanism validation, and even the development of new dosage forms in the future.
Transcranial Photosensitizer-Free Laser Treatment of Glioblastoma in Rat Brain
Authors: Semyachkina-Glushkovskaya Oxana , Sokolovski Sergey , Fedosov Ivan , Shirokov Alexander , Navolokin Nikita , Bucharskaya Alla , Blokhina Inna , Terskov Andrey , Dubrovski Alexander , Telnova Valeria , Tzven Anna , Tzoy Maria , Evsukova Arina , Zhlatogosrkaya Daria , Adushkina Viktoria , Dmitrenko Alexander , Manzhaeva Maria , Krupnova Valeria , Noghero Alessio , Bragin Denis , Bragina Olga , Borisova Ekaterina , Kurths Jürgen , Rafailov Edik
Published: September 2023

Over sixty years, laser technologies have undergone a technological revolution and become one of the main tools in biomedicine, particularly in neuroscience, neurodegenerative diseases and brain tumors. Glioblastoma is the most lethal form of brain cancer, with very limited treatment options and a poor prognosis. In this study on rats, we demonstrate that glioblastoma (GBM) growth can be suppressed by photosensitizer-free laser treatment (PS-free-LT) using a quantum-dot-based 1267 nm laser diode. This wavelength, highly absorbed by oxygen, is capable of turning triplet oxygen to singlet form. Applying 1267 nm laser irradiation for a 4 week course with a total dose of 12.7 kJ/cm2 firmly suppresses GBM growth and increases survival rate from 34% to 64%, presumably via LT-activated apoptosis, inhibition of the proliferation of tumor cells, a reduction in intracranial pressure and stimulation of the lymphatic drainage and clearing functions. PS-free-LT is a promising breakthrough technology in non- or minimally invasive therapy for superficial GBMs in infants as well as in adult patients with high photosensitivity or an allergic reaction to PSs.
Association of Phosphorylated Pyruvate Dehydrogenase with Pyruvate Kinase M2 Promotes PKM2 Stability in Response to Insulin
Authors: Hossain Abu Jubayer , Islam Rokibul , Seo Jong-Bok , Park Hwee-Seon , Kim Jong-Il , Kumar Vikas , Lee Keun Woo , Park Jae-Bong
Published: September 2023

Insulin is a crucial signalling molecule that primarily functions to reduce blood glucose levels through cellular uptake of glucose. In addition to its role in glucose homeostasis, insulin has been shown to regulate cell proliferation. Specifically, insulin enhances the phosphorylation of pyruvate dehydrogenase E1α (PDHA1) at the Ser293 residue and promotes the proliferation of HepG2 hepatocellular carcinoma cells. Furthermore, we previously observed that p-Ser293 PDHA1 bound with pyruvate kinase M2 (PKM2) as confirmed by coimmunoprecipitation. In this study, we used an in silico analysis to predict the structural conformation of the two binding proteins. However, the function of the protein complex remained unclear. To investigate further, we treated cells with si-PDHA1 and si-PKM2, which led to a reduction in PKM2 and p-Ser293 PDHA1 levels, respectively. Additionally, we found that the PDHA S293A dephospho-mimic reduced PKM2 levels and its associated enzyme activity. Treatment with MG132 and leupeptin impeded the PDHA1 S293A-mediated PKM2 reduction. These results suggest that the association between p-PDHA1 and PKM2 promotes their stability and protects them from protein degradation. Of interest, we observed that p-PDHA1 and PKM2 were localized in the nucleus in liver cancer patients. Under insulin stimulation, the knockdown of both PDHA1 and PKM2 led to a reduction in the expression of common genes, including KDMB1. These findings suggest that p-PDHA1 and PKM2 play a regulatory role in these proteins’ expression and induce tumorigenesis in response to insulin.
Advanced Optical Microscopy: Unveiling Functional Insights Regarding a Novel PPP2R1A Variant and Its Unreported Phenotype
Authors: Roldán Mònica , Nolasco Gregorio Alexander , Armengol Lluís , Frías Marcos , Morell Marta , García-Aragonés Manel , Epifani Florencia , Muchart Jordi , Ramírez-Almaraz María Luisa , Martorell Loreto , Hernando-Davalillo Cristina , Urreizti Roser , Serrano Mercedes
Published: September 2023

The number of genes implicated in neurodevelopmental conditions is rapidly growing. Recently, variants in PPP2R1A have been associated with syndromic intellectual disability and a consistent, but still expanding, phenotype. The PPP2R1A gene encodes a protein subunit of the serine/threonine protein phosphatase 2A enzyme, which plays a critical role in cellular function. We report an individual showing pontocerebellar hypoplasia (PCH), microcephaly, optic and peripheral nerve abnormalities, and an absence of typical features like epilepsy and an abnormal corpus callosum. He bears an unreported variant in an atypical region of PPP2R1A. In silico studies, functional analysis using immunofluorescence, and super-resolution microscopy techniques were performed to investigate the pathogenicity of the variant. This analysis involved a comparative analysis of the patient’s fibroblasts with both healthy control cells and cells from an individual with the previously described phenotype. The results showed reduced expression of PPP2R1A and the presence of aberrant protein aggregates in the patient’s fibroblasts, supporting the pathogenicity of the variant. These findings suggest a potential association between PPP2R1A variants and PCH, expanding the clinical spectrum of PPP2R1A-related neurodevelopmental disorder. Further studies and descriptions of additional patients are needed to fully understand the genotype–phenotype correlation and the underlying mechanisms of this novel phenotype.
Activation of G-Protein-Coupled Estrogen Receptor 1 (GPER1) Reduces Progression of Vulvar Carcinoma Cells
Authors: Loris Johanna , Hanesch Lena , Bauerschmitz Gerd , Gallwas Julia , Gründker Carsten
Published: September 2023

Whether G protein-coupled estrogen receptor 1 (GPER1) is tumor-promoting or tumor-suppressive depends in part on tumor entity. Little is known about the function of GPER1 in vulvar carcinoma. In this work, we aim to clarify what role GPER1 plays in vulvar cancer, tumor-promoting or tumor-suppressive. Localization of GPER1 in A431 and CAL-39 vulvar carcinoma cells was examined by immunofluorescence. Using a tissue microarray of vulvar neoplasias, the correlation between GPER1 expression and grade of malignancy was investigated. A431 and CAL-39 cells were treated either with GPER1 agonist G1 or antagonist G36. Proliferation was quantified by BrdU assay and viability examined using Resazurin assay. Morphological changes were analyzed by microscopy and measured using ImageJ. Cell migration was analyzed by gap closure assay. Clonogenic potential was tested by colony and sphere formation. Expression of estrogen receptors was examined by Western blot. GPER1 was found consistently expressed in vulvar neoplasia tissues. The immune-reactive score was found to be significantly higher in tissue samples of lymph node metastases and neoplasias with grade 3. In A431 and CAL-39 vulvar carcinoma cells, GPER1 expression was mainly found in the cytoplasm and nuclei. Treatment of A431 and CAL-39 cells with GPER1 agonist G1 resulted in a decrease in proliferation and migration. In addition, colony formation and tumor sphere formation were reduced. Furthermore, morphological signs of necrosis and reduction in cell viability after G1 treatment were observed. The GPER1 antagonist G36 did not have significant effects on vulvar carcinoma cells. Neither agonist G1 nor antagonist G36 treatment resulted in altered expression of estrogen receptors. Activation of GPER1 with GPER1 agonist G1 reduces the tumorigenic potential of the vulvar carcinoma cells. It can be deduced from this that GPER1 appears to have a tumor-suppressive effect in vulvar carcinoma.
A Two-Step Transcriptome Analysis of the Human Heart Reveals Broad and Disease-Responsive Expression of Ectopic Olfactory Receptors
Authors: Ashraf Sadia , Frazier O. Howard , Carranza Sylvia , McPherson David D. , Taegtmeyer Heinrich , Harmancey Romain
Published: September 2023

G-protein-coupled receptors (GPCRs) are critical regulators of cardiac physiology and a key therapeutic target for the treatment of heart disease. Ectopic olfactory receptors (ORs) are GPCRs expressed in extra-nasal tissues which have recently emerged as new mediators in the metabolic control of cardiac function. The goals of this study were to profile OR gene expression in the human heart, to identify ORs dysregulated by heart failure caused by ischemic cardiomyopathy, and to provide evidence suggestive of a role for those altered ORs in the pathogenesis of heart failure. Left ventricular tissue from heart failure patients (n = 18) and non-failing heart samples (n = 4) were subjected to a two-step transcriptome analysis consisting of the quantification of 372 distinct OR transcripts on real-time PCR arrays and simultaneous determination of global cardiac gene expression by RNA sequencing. This strategy led to the identification of >160 ORs expressed in the human heart, including 38 receptors differentially regulated with heart failure. Co-expression analyses predicted the involvement of dysregulated ORs in the alteration of mitochondrial function, extracellular matrix remodeling, and inflammation. We provide this dataset as a resource for investigating roles of ORs in the human heart, with the hope that it will assist in the identification of new therapeutic targets for the treatment of heart failure.
Chitosan and Its Derivatives: Preparation and Antibacterial Properties
Authors: Egorov Anton R. , Kirichuk Anatoly A. , Rubanik Vasili V. , Rubanik Vasili V. , Tskhovrebov Alexander G. , Kritchenkov Andreii S.
Published: September 2023

This comprehensive review illuminates the various methods of chitosan extraction, its antibacterial properties, and its multifarious applications in diverse sectors. We delve into chemical, physical, biological, hybrid, and green extraction techniques, each of which presents unique advantages and disadvantages. The choice of method is dictated by multiple variables, including the desired properties of chitosan, resource availability, cost, and environmental footprint. We explore the intricate relationship between chitosan’s antibacterial activity and its properties, such as cationic density, molecular weight, water solubility, and pH. Furthermore, we spotlight the burgeoning applications of chitosan-based materials like films, nanoparticles, nonwoven materials, and hydrogels across the food, biomedical, and agricultural sectors. The review concludes by highlighting the promising future of chitosan, underpinned by technological advancements and growing sustainability consciousness. However, the critical challenges of optimizing chitosan’s production for sustainability and efficiency remain to be tackled.
Pyrolysis Kinetic Behavior and Thermodynamic Analysis of PET Nonwoven Fabric
Authors: Yousef Samy , Eimontas Justas , Striūgas Nerijus , Mohamed Alaa , Ali Abdelnaby Mohammed
Published: September 2023

This research aims to maximize polyethylene terephthalate (PET) nonwoven fabric waste and make it as a new source for benzoic acid extraction using a pyrolysis process. The treatment was performed using a thermogravimetric analyzer (TGA) and released products were characterized using FTIR spectroscopy and gas chromatography–mass spectrometry (GC–MS). The pyrolysis kinetic and thermodynamic behavior of PET fabric was also studied and simulated using different linear and nonlinear models. The results show that the PET fabric is very rich in volatile matter (80 wt.%) and can completely degrade under 490 °C with a weight loss of 84%. Meanwhile, the generated vapor was rich in the carbonylic C=O functional group (FTIR), and the GC–MS analysis concluded that benzoic acid was the major compound with an abundance of 75% that was achieved at the lowest heating rate (5 °C/min). The linear kinetic results showed that PET samples had an activation energy in the ranges of 193–256 kJ/mol (linear models) and ~161 kJ/mol (nonlinear models). The thermodynamic parameters, including enthalpy, Gibbs free energy, and entropy, were estimated in the ranges of 149–250 kJ/mol, 153–232 kJ/mol, and 256–356 J/mol K, respectively. Accordingly, pyrolysis treatment can be used to extract benzoic acid from PET fabric waste with a 134% increase in the benzoic acid abundance that can be recovered from PET bottle plastic waste.
Preparation, Features, and Efficiency of Nanocomposite Fertilisers Based on Glauconite and Ammonium Dihydrogen Phosphate
Authors: Rudmin Maxim , Makarov Boris , López-Quirós Adrián , Maximov Prokopiy , Lokteva Valeria , Ibraeva Kanipa , Kurovsky Alexander , Gummer Yana , Ruban Alexey
Published: September 2023

This paper studies the chemical and mechanochemical preparation of glauconite with ammonium dihydrogen phosphate (ADP) nanocomposites with a ratio of 9:1 in the vol.% and wt.%, respectively. The methods include X-ray diffraction analysis, scanning electron microscope with energy-dispersive X-ray spectroscopy, transmission electron microscopy, infrared spectroscopy, and differential thermal analysis with a quadruple mass spectrometer. The manufactured nanocomposites keep the flaky glauconite structure. Some glauconite unit structures have been thickened due to minimal nitrogen (ammonium) intercalation into the interlayer space. The globular, granular, or pellet mineral particles of nanocomposites can be preserved via chemical techniques. Globular and micro-aggregate particles in nanocomposites comprise a thin film of adsorbed ADP. The two-step mechanochemical method makes it possible to slightly increase the proportion of adsorbed (up to 3.2%) and intercalated (up to 6.0%) nutrients versus chemical ways. Nanocomposites prepared via chemical methods consist of glauconite (90%), adsorbed (1.8–3.6%), and intercalated (3.0–3.7%) substances of ADP. Through the use of a potassium-containing clay mineral as an inhibitor, nitrogen, phosphorus, and potassium (NPK), nanocomposite fertilisers of controlled action were obtained. Targeted and controlled release of nutrients such as phosphate, ammonium, and potassium are expected due to various forms of nutrients on the surface, in the micropores, and in the interlayer space of glauconite. This is confirmed via the stepwise dynamics of the release of ammonium, nitrate, potassium, and phosphate from their created nanocomposites. These features of nanocomposites contribute to the stimulation of plant growth and development when fertilisers are applied to the soil.
Palladium–Copper Bimetallic Aerogel as New Modifier for Highly Sensitive Determination of Bisphenol A in Real Samples
Authors: Fang Zehao , Wang Junyan , Xue Yilei , Khorasani Motlagh Mozhgan , Noroozifar Meissam , Kraatz Heinz-Bernhard
Published: September 2023

In this study, a bimetallic palladium–copper aerogel was synthesized and used for modification of a graphite paste electrode (Pd-Cu/GPE), allowing the sensitive determination of bisphenol A (BPA). Different techniques, such as SEM, TEM, XPS, and AFM, were used for characterization of the Pd-Cu aerogel. To elucidate the properties of the Pd-Cu/GPE, the electrochemistry methods such as differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy were used. DPV measurements were conducted in phosphate electrolyte and buffer solution (0.2 M PBS, pH 5) at a potential range from 0.4 to 0.9 V vs. Ag/AgCl. The DPVs peaks currents increased linearly with BPA concentrations in the 0.04–85 and 85–305 µM ranges, with a limit of detection of 20 nM. The modified electrode was successfully used in real samples to determine BPA, and the results were compared to the standard HPLC method. The results showed that the Pd-Cu/GPE had good selectivity, stability, and sensitivity for BPA determination.
The Impact of COVID-19 Pandemic on Weight Loss, Eating Behaviour and Quality of Life after Roux-en-Y Gastric Bypass
Authors: Bauraitė Karolina , Gudaitytė Rita , Maleckas Almantas
Published: September 2023

Background and Objectives: The global pandemic of coronavirus disease (COVID-19), declared on 11 March 2020, had an extensive impact on bariatric patients. The aim of this study was to evaluate short-term weight loss outcomes, changes in eating behaviour, and health-related quality of life (HRQoL) among patients who had Roux-en-Y gastric bypass (RYGB) before and during the COVID-19 pandemic. Materials and Methods: This cohort study included 72 patients (Group S) who underwent RYGB surgery in the Surgery Department of the Lithuanian University of Health Sciences during the COVID-19 pandemic in the years 2020–2022. Data for the control group (Group C) of 87 patients (operated on in 2010–2012) were collected from a prospective study. The data referred to the period before and a year after the RYGB. The information about patients’ weight changes, hunger, satiety, fullness sensations, appetite, diet, and eating patterns was queried. Eating behaviour and HRQoL evaluation were conducted by the Three-Factor Eating Questionnaire (TFEQ-R18) and the medical outcomes study Short-Form-36 (SF-36), respectively. Results: One year after the surgery, % excess body mass index loss (%EBMIL) was 77.88 (26.33) in Group S, 76.21 (19.98) in Group C, p = 0.663. Patients in Group S tended more to choose snacks between main meals: 79.2% versus 28.7%, p < 0.0001. Cognitive restraint significantly increased in Group S from 45.93 (13.37) up to 54.48 (13.76), p = 0.001; additionally, significantly worse overall health status was found in Group S compared to Group C, 53.27 (24.61) versus 70.11 (31.63), p < 0.0001. Mental HRQoL (50.76 versus 60.52 score, p < 0.0001) and social functioning (44.79 versus 57.90, p < 0.0001) were worse in Group S. Conclusions: In this study, the COVID-19 pandemic had no impact on short-term weight loss after RYGB. However, one year after, RYGB patients tended to snack more, and mental HRQoL and social functioning were worse in the study group.
A Multi-Dimensional Calibration Based on Genetic Algorithm in a 12-Bit 750 MS/s Pipelined ADC
Authors: Jia Hanbo , Guo Xuan , Zhai Huaiyu , Wu Feitong , Zhang Yuzhen , Wang Dandan , Sun Kai , Wu Danyu , Liu Xinyu
Published: September 2023

As the preferred architecture for high-speed and high-resolution analog-to-digital converters (ADC), the accuracy of pipelined ADC is limited mainly by various errors arising from multiple digital-to-analog converters (MDAC). This paper presents a multi-dimensional (M-D) MDAC calibration based on a genetic algorithm (GA) in a 12-bit 750 MS/s pipelined ADC. The proposed M-D MDAC compensation model enables capacitor mismatch and static interstage gain error (IGE) compensation on the chip and prepares for subsequent background calibration based on a pseudo-random number (PN) injection to achieve accurate compensation for dynamic IGE. An M-D coefficient extraction scheme based on GA is also proposed to extract the required compensation coefficients of the foreground calibration, which avoids falling into local traps through MATLAB. The above calibration scheme has been verified in a prototype 12-bit 750 MS/s pipelined ADC. The measurement results show that the signal-to-noise and distortion ratio (SNDR) and spurious-free dynamic range (SFDR) are increased from 49.9 dB/66.7 dB to 59.6 dB/77.5 dB with the proposed calibration at 25 °C. With the help of background calibration at 85 °C, the SNDR and SFDR are improved by 3.4 dB and 8.8 dB, respectively.