bluuebunny/crossref_metadata_2025 huggingface dataset:
Algorithmic Design in Virtual Reality
Authors: Castelo-Branco Renata , Leitão António
Published: February 2022

Virtual reality has been shown to facilitate perception and navigation inside 3D models, while stimulating creativity and enhancing architect/client interaction. In this scenario, in order to better explore paths along the design space that are suggested from this interaction, it is important to support quick updates to the model while still immersed in it. Algorithmic design, an approach to architectural design that uses parametric algorithms to represent a design space, rather than a single design instance, provides such support. We present a novel architectural design process based on the integration of live coding with virtual reality, promoting an immersive approach to algorithmic design. The proposed workflow entails the use of an algorithmic design tool embedded in a virtual environment, where the architect not only creates the design but also interacts with said design, changing it by live coding its algorithmic representation from within virtual reality. In this paper, we explain the challenges faced and solutions devised for the implementation of the proposed workflow. Moreover, we discuss the applicability of algorithmic design in virtual reality to different stages of the architectural design process and the future developments that may arise from this proposal.
Adaptation of a Bacterial Bioluminescent Assay to Monitor Bioeffects of Gold Nanoparticles
Authors: Yehia Moustafa R. , Smolyarova Tatyana E. , Shabanov Alexandr V. , Sushko Ekaterina S. , Badun Gennady A. , Kudryasheva Nadezhda S.
Published: February 2022

Our current study aimed to adapt a bioluminescent bacteria-based bioassay to monitor the bioeffects of gold nanoparticles (AuNPs). Luminous marine bacteria Photobacterium phosphoreum and AuNPs modified with polyvinylpyrrolidone were employed; low-concentration (≤10−3 g/L) bioeffects of AuNPs were studied. Bioluminescence intensity was used as an indicator of physiological activity in bacteria. Two additional methods were used: reactive oxygen species (ROS) content was estimated with a chemiluminescent luminol method, and bacterial size was monitored using electron microscopy. The bacterial bioluminescent response to AuNPs corresponded to the “hormesis” model and involved time-dependent bioluminescence activation, as well as a pronounced increase in the number of enlarged bacteria. We found negative correlations between the time courses of bioluminescence and the ROS content in bacterial suspensions, demonstrating the relationship between bioluminescence activation and bacterial ROS consumption. The combined effects of AuNPs and a beta-emitting radionuclide, tritium, revealed suppression of bacterial bioluminescent activity (as compared to their individual effects) and a reduced percentage of enlarged bacteria. Therefore, we demonstrated that our bacteria-based bioluminescence assay is an appropriate tool to study the bioeffects of AuNPs; the bioeffects can be further classified within a unified framework for rapid bioassessment.
Preclinical Identification of Sulfasalazine’s Therapeutic Potential for Suppressing Colorectal Cancer Stemness and Metastasis through Targeting KRAS/MMP7/CD44 Signaling
Authors: Leung Wai-Hung , Shih Jing-Wen , Chen Jian-Syun , Mokgautsi Ntlotlang , Wei Po-Li , Huang Yan-Jiun
Published: February 2022

Approximately 25% of colorectal cancer (CRC) patients will develop metastatic (m)CRC despite treatment interventions. In this setting, tumor cells are attracted to the epidermal growth factor receptor (EGFR) oncogene. Kirsten rat sarcoma (RAS) 2 viral oncogene homolog (KRAS) mutations were reported to drive CRC by promoting cancer progression in activating Wnt/β-catenin and RAS/extracellular signal-regulated kinase (ERK) pathways. In addition, KRAS is associated with almost 40% of patients who acquire resistance to EGFR inhibitors in mCRC. Multiple studies have demonstrated that cancer stem cells (CSCs) promote tumorigenesis, tumor growth, and resistance to therapy. One of the most common CSC prognostic markers widely reported in CRC is a cluster of differentiation 44 (CD44), which regulates matrix metalloproteinases 7/9 (MMP7/9) to promote tumor progression and metastasis; however, the molecular role of CD44 in CRC is still unclear. In invasive CRC, overexpression of MMP7 was reported in tumor cells compared to normal cells and plays a crucial function in CRC cetuximab and oxaliplatin resistance and distant metastasis. Here, we utilized a bioinformatics analysis and identified overexpression of KRAS/MMP7/CD44 oncogenic signatures in CRC tumor tissues compared to normal tissues. In addition, a high incidence of mutations in KRAS and CD44 were associated with some of the top tumorigenic oncogene’s overexpression, which ultimately promoted a poor response to chemotherapy and resistance to some FDA-approved drugs. Based on these findings, we explored a computational approach to drug repurposing of the drug, sulfasalazine, and our in silico molecular docking revealed unique interactions of sulfasalazine with the KRAS/MMP7/CD44 oncogenes, resulting in high binding affinities compared to those of standard inhibitors. Our in vitro analysis demonstrated that sulfasalazine combined with cisplatin reduced cell viability, colony, and sphere formation in CRC cell lines. In addition, sulfasalazine alone and combined with cisplatin suppressed the expression of KRAS/MMP7/CD44 in DLD-1 and HCT116 cell lines. Thus, sulfasalazine is worthy of further investigation as an adjuvant agent for improving chemotherapeutic responses in CRC patients.
The Translational Bridge between Inflammation and Hepatocarcinogenesis
Authors: Gufler Sabine , Seeboeck Rita , Schatz Christoph , Haybaeck Johannes
Published: February 2022

Viral infections or persistent alcohol or drug abuse, together with intrinsic factors, lead to hepatitis, which often ends in the development of liver cirrhosis or hepatocellular carcinoma (HCC). With this review, we describe inflammatory liver diseases, such as acute liver failure, virus-induced hepatitis, alcoholic- and non-alcoholic steatohepatitis, and autoimmune hepatitis, and highlight their driving mechanisms. These include external factors such as alcohol misuse, viral infection and supernutrition, as well as intrinsic parameters such as genetic disposition and failure, in immune tolerance. Additionally, we describe what is known about the translational machinery within all these diseases. Distinct eukaryotic translation initiation factors (eIFs) with specific functional roles and aberrant expression in HCC are reported. Many alterations to the translational machinery are already triggered in the precancerous lesions described in this review, highlighting mTOR pathway proteins and eIFs to emphasize their putative clinical relevance. Here, we identified a lack of knowledge regarding the roles of single eIF proteins. A closer investigation will help to understand and treat HCC as well as the antecedent diseases.
ISG15 and ISGylation in Human Diseases
Authors: Mirzalieva Oygul , Juncker Meredith , Schwartzenburg Joshua , Desai Shyamal
Published: February 2022

Type I Interferons (IFNs) induce the expression of >500 genes, which are collectively called ISGs (IFN-stimulated genes). One of the earliest ISGs induced by IFNs is ISG15 (Interferon-Stimulated Gene 15). Free ISG15 protein synthesized from the ISG15 gene is post-translationally conjugated to cellular proteins and is also secreted by cells into the extracellular milieu. ISG15 comprises two ubiquitin-like domains (UBL1 and UBL2), each of which bears a striking similarity to ubiquitin, accounting for its earlier name ubiquitin cross-reactive protein (UCRP). Like ubiquitin, ISG15 harbors a characteristic β-grasp fold in both UBL domains. UBL2 domain has a conserved C-terminal Gly-Gly motif through which cellular proteins are appended via an enzymatic cascade similar to ubiquitylation called ISGylation. ISG15 protein is minimally expressed under physiological conditions. However, its IFN-dependent expression is aberrantly elevated or compromised in various human diseases, including multiple types of cancer, neurodegenerative disorders (Ataxia Telangiectasia and Amyotrophic Lateral Sclerosis), inflammatory diseases (Mendelian Susceptibility to Mycobacterial Disease (MSMD), bacteriopathy and viropathy), and in the lumbar spinal cords of veterans exposed to Traumatic Brain Injury (TBI). ISG15 and ISGylation have both inhibitory and/or stimulatory roles in the etiology and pathogenesis of human diseases. Thus, ISG15 is considered a “double-edged sword” for human diseases in which its expression is elevated. Because of the roles of ISG15 and ISGylation in cancer cell proliferation, migration, and metastasis, conferring anti-cancer drug sensitivity to tumor cells, and its elevated expression in cancer, neurodegenerative disorders, and veterans exposed to TBI, both ISG15 and ISGylation are now considered diagnostic/prognostic biomarkers and therapeutic targets for these ailments. In the current review, we shall cover the exciting journey of ISG15, spanning three decades from the bench to the bedside.
Evaluation of the Versius Robotic Surgical System for Procedures in Small Cavities
Authors: Kayser Marit , Krebs Thomas Franz , Alkatout Ibrahim , Kayser Timo , Reischig Katja , Baastrup Jonas , Meinzer Andreas , Ulrich Katja , Osmonov Daniar , Bergholz Robert
Published: February 2022

Background: The Versius® is a recently approved robotic surgical system for general surgery procedures in adults. Before any application in children, data of its feasibility and safety in small cavities has to be compiled, beginning with inanimate models. Therefore, the aim of this preclinical study was to assess the Versius® system for its performance in small boxes simulating small body cavities. Methods: In total, 8 cardboard boxes of decreasing volumes (15.75 L to 106 mL) were used. The procedures, two single stitches with two square knots each, were performed in every box, starting in the largest and consecutively exchanging the box to the next smaller one. The evaluation included procedure time, port placement and pivot point setup, arrangement of the robotic arms and instrumentation, amount of internal and external instrument–instrument collisions and instrument–box collisions. Results: All procedures could be successfully performed in all boxes. The procedure time decreased due to the learning curve in the first four boxes (15.75 to 1.87 L) and consecutively increased from boxes of 1.22 L up to the smallest box with the dimensions of 4.4 × 4.9 × 4.9 cm3. This may be based on the progress of complexity of the procedures in small cavities, which is also depicted by the synchronous increase of the internal instrument–instrument and instrument–box collisions. Conclusion: With the use of the Versius® robotic surgical system, we were able to perform robotic reconstructive procedures, such as intracorporal suturing and knot tying, in cavities as small as 106 mL. Whether this system is comparable or even superior to conventional laparoscopic surgery in small cavities, such as in children, has to be evaluated. Furthermore, before any application in newborns or infants, ongoing evaluation of this system should be performed in a live animal model.
Family Environment and Portuguese Adolescents: Impact on Quality of Life and Well-Being
Authors: Botelho Guedes Fábio , Cerqueira Ana , Gaspar Susana , Gaspar Tania , Moreno Carmen , Gaspar de Matos Margarida
Published: February 2022

Background: A healthy and supportive family environment leads to more positive results regarding adolescents’ development. The main objective of this study was to explore and analyze the relationship between adolescents’ quality of life (QoL) and their family environment/relationship. Method: The sample was collected as part of the Health Behavior in School-aged Children 2018 study, which included 8215 adolescents, 52.7% female, with a mean age of 14.36 years (SD = 2.28). Results: Girls are more involved in family activities (such as family meals), report being treated with fairness by their parents and feel less parental pressure to get good grades. Boys have a higher perception regarding their family affluence, better family relationships and support and better QoL. Having an above-average QoL is significantly related to high family affluence, better communication with both parents, greater involvement in family activities, greater perception of help from parents regarding decision-making, greater perception of being treated with fairness by parents and less pressure from parents to get good grades, as well as a better family relationship/support. Conclusion: It is important to determine the impact that parental divorce/separation or a weak parent–child relationship can have on adolescents. It is also necessary to consider the family relationship and structure when devising strategies and public policies related to the promotion of adolescents’ health and well-being.
Short Mindfulness-Based Relaxation Training Has No Effects on Executive Functions but May Reduce Baseline Cortisol Levels of Boys in First Grade: A Pilot Study
Authors: Koncz Adam , Kassai Reka , Demetrovics Zsolt , Takacs Zsofia K.
Published: February 2022

(1) Background: Executive functions are important for academic performance and school readiness. Children’s executive function skills are found to be improved by mindfulness-based interventions, and these programs are also effective in stress reduction. The aim of this study was to evaluate the feasibility and the effects of a short mindfulness-based relaxation training compared to a passive control condition right before school entry on executive function skills and cortisol levels. (2) Methods: The feasibility and the effects of the intervention before school entry were tested with 61 preschoolers. The final sample consisted of 51 participants (Mage = 81.90 months, SD = 5.45; 41% male). Short-term memory, executive function skills and cortisol levels before and after the intervention were assessed. Additionally, cortisol levels were assessed one week and one month after school entry. (3) Results: There was a significant sex difference in the effects of the intervention on children’s cortisol levels (p = 0.026, η2 = 0.134). The mindfulness-based relaxation training applied before school entry prevented a rise in boys’ cortisol levels one week after starting school. (4) Conclusion: A short mindfulness-based intervention before starting school could be effective in fostering physiological stress management in boys.
Molecular Characterization of Two Genes Encoding Novel Ca2+-Independent Phospholipase A2s from the Silkworm, Bombyx mori
Authors: Hu Xin , Zhang Bili , Zheng Xi , Ji Haoyan , Feng Kun , Hu Xiaosong , Gul Isma , Abbas Muhammad Nadeem , Cui Hongjuan , Zhu Yong
Published: February 2022

Eicosanoids are crucial downstream signals in the insect immune responses. Phospholipase A2 (PLA2) catalyzes phospholipids, the initial step in eicosanoid biosynthesis. In mammals, the biological roles of Ca2+-independent Phospholipase A2 (iPLA2) have been extensively studied; however, only a few studies have attempted to explore iPLA2 functions in insects. In this study, we identified two iPLA2 genes (designated as BmiPLA2A and BmiPLA2B) in the silkworm, Bombyx mori. BmiPLA2A had a 2427 base pair (bp) open reading frame (ORF) that coded for a protein with 808 amino acids. In contrast, BmiPLA2B had a 1731 bp ORF that coded for a protein with 576 amino acids. Domain analysis revealed that BmiPLA2A had six ankyrin repeat domains, but BmiPLA2B lacks these domains. BmiPLA2A and BmiPLA2B were transcribed widely in various tissues and developmental stages with different expression patterns. The administration of 20-hydroxyecdysone increased their expression levels in the epidermis and hemocytes. Furthermore, challenged with virus, fungus, Gram-negative bacteria, and Gram-positive bacteria induced the expression of BmiPLA2A and BmiPLA2B with variable degrees along with different time points. Our findings imply that BmiPLA2A and BmiPLA2B may have important biological roles in the development and innate immunity of B. mori.
Assessment of Metabolic Adaptations in Periparturient Dairy Cows Provided 3-Nitrooxypropanol and Varying Concentrate Proportions by Using the GreenFeed System for Indirect Calorimetry, Biochemical Blood Parameters and Ultrasonography of Adipose Tissues
Authors: Schilde Matthias , von Soosten Dirk , Frahm Jana , Kersten Susanne , Meyer Ulrich , Zeyner Annette , Dänicke Sven
Published: February 2022

Methanogenesis in ruminants contributes to both greenhouse gas emissions and feed energy losses whereby the latter becomes specifically important in energy-deficient periparturient cows. It was hypothesized that increased concentrate feed proportions (CFP) and feeding with the methane inhibitor 3-nitrooxypropanol (3-NOP), as well as their potential synergism, improve the energy status of peripartal cows. Periparturient dairy cows were fed low or high dietary CFP either tested without or combined with 3-NOP. The GreenFeed system was used to calculate the metabolic respiration quotient (RQmetabolic) and tissue energy retention (ERtissue) by methods of indirect calorimetry. The calorimetrically estimated ERtissue coincided with a conventionally calculated energy balance except for the antepartal period. Neither CFP nor 3-NOP affected the ultrasonographically assessed lipomobilization in adipose depots. In the group fed 3-NOP and a high concentrate feed proportion, the RQmetabolic significantly rose over the course of the experiment and the ERtissue was also increased. Serum non-esterified fatty acid concentrations were lower in the 3-NOP groups albeit ß-hydroxybutyrate (BHB) remained unaffected. Higher CFP reduced BHB and increased blood glucose levels. In conclusion, 3-NOP and high CFP improved the energy budget of the cows in an interactive manner, which was, however, not apparent in all of the examined parameters. The application of the GreenFeed system for indirect calorimetry is a promising approach, which needs further validation in the future.
The Use of Weather Radar Data: Possibilities, Challenges and Advanced Applications
Authors: Binetti Maria Silvia , Campanale Claudia , Massarelli Carmine , Uricchio Vito Felice
Published: February 2022

The climate in recent decades has aroused interest in the scientific community, prompting us to analyse the mechanisms that regulate it, to understand the climate change responsible for an increase in extreme phenomena. Consequently, the increase in hydrogeological instability in the Italian territory has led to an in-depth study of atmospheric parameters to understand the variations of the atmospheric system. One tool capable of detecting such variations is the weather radar. The weather radar data available in the area provided by the National Radar Network of the Department of Civil Protection allow the evaluation of variations on a national scale for hydro-meteorological-climatic monitoring as well as the disasters that have occurred. Using open-source programming software, the servers can be queried and data retrieved from a source to perform processing for specific purposes through data extraction techniques.
Effect of Battery Degradation on the Probabilistic Optimal Operation of Renewable-Based Microgrids
Authors: Javidsharifi Mahshid , Pourroshanfekr Arabani Hamoun , Kerekes Tamas , Sera Dezso , Spataru Sergiu , Guerrero Josep M.
Published: February 2022

In order to maximize the use of renewable-based distributed generators (DGs), in addition to dealing with the effects of the inherent power management uncertainties of microgrids (MGs), applying storage devices is essential in the electrical system. The main goal of this paper is to minimize the total operation cost as well as the emissions of MG energy resources, alongside the better utilization of renewable energy sources (RES) and energy storage systems. The uncertainties of wind speed, solar irradiation, market price and electrical load demand are modeled using reduced unscented transformation (RUT) method. Simulation results reveal that, as expected, by increasing the battery efficiency, the achievable minimum daily operational cost of the system is reduced. For example, with 93% battery efficiency, the operational cost equals EUR 9200, while for an efficiency of 97%, the achievable minimum daily operational cost is EUR 8900. Moreover, the proper economic/environmental performance of the suggested approach, which contributes to the possibility of selecting a compromise solution for the MG operator in accordance with technical and economic constraints, is justified.
Passive Intermodulation at Contacts of Rough Conductors
Authors: Dayan Amir , Huang Yi , Schuchinsky Alex
Published: February 2022

Passive intermodulation (PIM) is a niggling phenomenon that debilitates the performance of modern communications and navigation systems. PIM products interfere with information signals and cause their nonlinear distortion. The sources and basic mechanisms of PIM have been studied in the literature but PIM remains a serious problem of signal integrity. In this paper, the main sources and mechanisms of PIM generation by joints of good conductors are discussed. It is shown that the passive electrical, thermal and mechanical nonlinearities are intrinsically linked despite their distinctively different time scales. The roughness of the contact surfaces plays an important role in PIM generation by conductor joints. A review of the PIM phenomenology at the contacts of the good conductors suggests that novel multiphysics models are necessary for the analysis and reliable prediction of PIM products generated by several concurrent nonlinearities of a diverse physical nature.
Transient Anomalous Diffusion MRI Measurement Discriminates Porous Polymeric Matrices Characterized by Different Sub-Microstructures and Fractal Dimension
Authors: Palombo Marco , Barbetta Andrea , Cametti Cesare , Favero Gabriele , Capuani Silvia
Published: February 2022

Considering the current development of new nanostructured and complex materials and gels, it is critical to develop a sub-micro-scale sensitivity tool to quantify experimentally new parameters describing sub-microstructured porous systems. Diffusion NMR, based on the measurement of endogenous water’s diffusion displacement, offers unique information on the structural features of materials and tissues. In this paper, we applied anomalous diffusion NMR protocols to quantify the subdiffusion of water and to measure, in an alternative, non-destructive and non-invasive modality, the fractal dimension dw of systems characterized by micro and sub-micro geometrical structures. To this end, three highly heterogeneous porous-polymeric matrices were studied. All the three matrices composed of glycidylmethacrylate-divynilbenzene porous monoliths obtained through the High Internal Phase Emulsion technique were characterized by pores of approximately spherical symmetry, with diameters in the range of 2–10 μm. Pores were interconnected by a plurality of window holes present on pore walls, which were characterized by size coverings in the range of 0.5–2 μm. The walls were characterized by a different degree of surface roughness. Moreover, complementary techniques, namely Field Emission Scanning Electron Microscopy (FE-SEM) and dielectric spectroscopy, were used to corroborate the NMR results. The experimental results showed that the anomalous diffusion α parameter that quantifies subdiffusion and dw = 2/α changed in parallel to the specific surface area S (or the surface roughness) of the porous matrices, showing a submicroscopic sensitivity. The results reported here suggest that the anomalous diffusion NMR method tested may be a valid experimental tool to corroborate theoretical and simulation results developed and performed for describing highly heterogeneous and complex systems. On the other hand, non-invasive and non-destructive anomalous subdiffusion NMR may be a useful tool to study the characteristic features of new highly heterogeneous nanostructured and complex functional materials and gels useful in cultural heritage applications, as well as scaffolds useful in tissue engineering.
The Role of Vitamin K in Cirrhosis: Do Pharmaco-K-Netics Matter?
Authors: Jin Stephanie , Hong Lisa , FakhriRavari Alireza
Published: February 2022

Patients with advanced liver disease who are not taking vitamin K antagonists often have an elevated international normalized ratio, potentially due to vitamin K deficiency and the decreased synthesis of clotting factors by the liver. It is possible that vitamin K deficiency is due to dietary deficiency, impaired absorption in the small intestine, or both. This has led to the practice of the administration of phytonadione to limit the risks of bleeding in these patients. However, phytonadione is available in different formulations with varying pharmacokinetics and there is a paucity of data in the literature to guide optimal management. The routine use of phytonadione to correct INR in cirrhotic patients not taking warfarin should be avoided due to the lack of proven benefits. However, intravenous phytonadione may be considered in actively bleeding or critically ill patients with vitamin K deficiency. Oral formulation is unlikely to be absorbed in cirrhotic patients and should be avoided.
Reliability Study of the Items of the Alberta Infant Motor Scale (AIMS) Using Kappa Analysis
Authors: Ko Jooyeon , Lim Hyun Kyoon
Published: February 2022

Purpose: We evaluated the interrater and intrarater reliabilities of the Korean version of the Alberta Infant Motor Scale (K-AIMS). Methods: For the interrater reliability test, six raters participated in the K-AIMS evaluation using video clips of 70 infants (aged between 0 and 18 months). One rater participated in an intrarater reliability test. Among 70 infants, 46 were born preterm and 24 were born full term. A total of 58 AIMS items were evaluated for supine, prone, sitting, and standing positions. A reliability analysis was conducted using ICC and Fleiss’ kappa. Results: The highest Fleiss’ kappa was found for the 4–7 months group for sitting (K = 0.701–1.000) and standing (K = 0.721–1.000), while the lowest K was the 3 months or under group for standing (K = 0.153–1.000). We found higher Fleiss’ kappa statistics when all infants were evaluated without grouping for the three positions (K = 0.727–1.000), except standing (K = 0.192–1.000), for the interrater analysis. Conclusion: Our results demonstrate the good reliability for the Korean version of the AIMS for Korean infants (preterm and full term).
The Incidence, Electrophysiological Characteristics and Ablation Outcome of Left Atrial Tachycardias after Pulmonary Vein Isolation Using Three Different Ablation Technologies
Authors: Leitz Patrick , Wasmer Kristina , Andresen Christian , Güner Fatih , Köbe Julia , Rath Benjamin , Reinke Florian , Wolfes Julian , Lange Philipp S. , Ellermann Christian , Frommeyer Gerrit , Eckardt Lars
Published: February 2022

Background: Left atrial tachycardias (LAT) are a well-known outcome of pulmonary vein isolation (PVI). Few data are available on whether the catheter used to perform PVI influences the incidence, as well as the characteristics of post PVI LAT. We present data on LAT following PVI by the following three ablation technologies: (1) phased multi-electrode radiofrequency catheter (PVAC), (2) irrigated single-tip catheter (iRF), and (3) cryoballoon ablation. Methods: Using a prospectively designed single-center database, we analyzed 650 patients (300 iRF, 150 PVAC, and 200 cryoballoon) with paroxysmal (n = 401) and persistent atrial fibrillation (AF), who underwent their first PVI at our center. Results: The three populations were comparable in their baseline characteristics; however, the cryoballoon group comprised a higher percentage of patients with persistent AF (p = 0.05). The LAT rates were 3.7% in the iRF group (mean follow-up 22 ± 14 months), 0.7% in the PVAC group (mean follow-up 21 ± 14 months), and 4% in the cryoballoon group (mean follow-up 15 ± 8 months). The predominant mechanism of LAT was macro-reentrant tachycardia. Reconnection of at least one pulmonary vein was observed in 87% of the patients who underwent 3D mapping. No predictors for LAT occurrence were identified. Conclusion: The occurrence of LAT post PVI is rare; the predominant mechanism was macro-reentrant tachycardia. Reconnection of at least one pulmonary vein was observed in nearly all the LAT patients. In our retrospective analysis, the lowest rate of LAT was observed with the PVAC. No predictors for LAT occurrence were identified.
Environmental Education in Zoos—Exploring the Impact of Guided Zoo Tours on Connection to Nature and Attitudes towards Species Conservation
Authors: Kleespies Matthias Winfried , Feucht Viktoria , Becker Martin , Dierkes Paul Wilhelm
Published: February 2022

In recent decades, zoos have been increasingly transformed into education centers with the goal of raising awareness about environmental issues and providing environmental education. Probably the simplest and most widespread environmental education program in the zoo is the guided tour. This study therefore aims to test whether a one hour zoo tour has an influence on the participants’ connection to nature and attitude towards species conservation. For this purpose, 269 people who had voluntarily registered for a zoo tour were surveyed before and after the tour. In addition to the regular zoo tour, special themed tours and tours with animal feedings were included. The results show a positive increase in connection to nature and a strengthening of positive attitudes towards species conservation for all tour types. For nature connectedness, in particular, people with an initial high connection to nature benefitted from the special themed tours and the tours, including animal feedings. For attitudes towards species conservation, no difference was found between the tour types. The results prove the positive influence of a very simple environmental education program, even for people with a preexisting high level of connection to nature and positive attitude towards species conservation.
Prone Positioning May Improve the Treatment of Diffuse Alveolar Hemorrhage and Severe Acute Respiratory Distress Syndrome (ARDS) Secondary to ANCA Associated Vasculitis: A Case Report
Authors: Wu Shang-Ju , Hsu Yong-Chen , Wang Kao-Lun , Fu Pin-Kuei
Published: February 2022

Diffuse alveolar hemorrhage (DAH) secondary to anti-neutrophil cytoplasmic antibodies (ANCA)-associated vasculitis is rare in clinical practice and may present as severe acute respiratory distress syndrome (ARDS) with high mortality. Extracorporeal membrane oxygenation (ECMO) has been reported to be a salvage treatment providing the time necessary for immunosuppressive treatment in cases accompanied by severe ARDS. Prone positioning (PP) has been proven to reduce the mortality in patients with severe ARDS. However, there is no consensus about choosing PP or ECMO in severe ARDS due to DAH secondary to ANCA-associated vasculitis. We reported a case of microscopic polyangiitis (MPA)-related DAH and severe ARDS treated with PP successfully providing the time necessary for early glucocorticoids and plasma exchange to control the underlying disease. Since anticoagulation therapy is not necessary in PP, it does not increase the risk of bleeding tendency unlike ECMO. PP has a life-saving role in the management of patients with severe ARDS due to ANCA-associated pulmonary vasculitis.
Local Linear Approximation Algorithm for Neural Network
Authors: Zeng Mudong , Liao Yujie , Li Runze , Sudjianto Agus
Published: February 2022

This paper aims to develop a new training strategy to improve efficiency in estimation of weights and biases in a feedforward neural network (FNN). We propose a local linear approximation (LLA) algorithm, which approximates ReLU with a linear function at the neuron level and estimate the weights and biases of one-hidden-layer neural network iteratively. We further propose the layer-wise optimized adaptive neural network (LOAN), in which we use the LLA to estimate the weights and biases in the LOAN layer by layer adaptively. We compare the performance of the LLA with the commonly-used procedures in machine learning based on seven benchmark data sets. The numerical comparison implies that the proposed algorithm may outperform the existing procedures in terms of both training time and prediction accuracy.
Impact of Sensorineural Hearing Loss during the Pandemic of COVID-19 on the Appearance of Depressive Symptoms, Anxiety and Stress
Authors: Zivkovic Marinkov Emilija M. , Rancic Natasa K. , Milisavljevic Dusan R. , Stankovic Milan D. , Milosevic Vuk D. , Malobabic Marina M. , Popovic Irena N. , Ignjatovic Aleksandra M. , Bojanovic Mila R. , Stojanovic Jasmina D.
Published: February 2022

Background. The incidence of hearing loss is constantly increasing and according to the World Health Organization, by 2050, 900 million people will suffer from hearing loss. The main Objective of the study was to determine the differences between the severity of the symptoms of stress, anxiety and depression in participants with varying degrees of sensorineural hearing loss during the COVID-19 pandemic. An additional aim was to examine the extent and manner in which protective face masks impact the communication of people with hearing loss. Matrials and Methods: A cross-sectional study was conducted, which included 160 patients (81 men and 79 women) with bilateral sensorineural hearing loss. The patients’ age range was 50 to 80 years. Depending on the degree of hearing loss or pure-tone threshold, the participants were divided into four groups: mild hearing loss, moderate hearing loss, severe hearing loss and profound hearing loss. The research used the Depression, Anxiety and Stress Scale (DASS-21) and a questionnaire in which the participants reported whether surgical face masks (medical three-layer masks) worn by speakers makes communication difficult, to what extent and in what way. Results: The average age of the patients was 67.97 ± 8.16. A significant correlation was found between the degree of hearing loss and communication difficulties caused by the use of protective face masks (p < 0.001). For patients with severe and profound hearing loss, communication is significantly more difficult (50.0% and 45.0% respectively) when the interlocutor wears a face mask. There is a significant correlation between the degree of hearing loss and the way in which communication is made more difficult when the interlocutor wears a face mask (p < 0.001). A statistically significant difference was determined between the degrees of hearing loss in all measured subscales: stress (p = 0.024), anxiety (p = 0.026) and depression (p = 0.016). Conclusions: We have determined that face masks used during the COVID-19 pandemic significantly hamper communication among the study groups (p = 0.007) and there is a significant correlation between the degree of sensorineural hearing loss and the presence of symptoms in all three DASS-21 subscales, meaning that the symptoms of stress, anxiety and depression were more intense in severe and profound hearing loss.
Differences in UV-C LED Inactivation of Legionella pneumophila Serogroups in Drinking Water
Authors: Buse Helen Y. , Hall John S. , Hunter Gary L. , Goodrich James A.
Published: February 2022

Legionella pneumophila (Lp) is an opportunistic pathogen that causes respiratory infections primarily through inhalation of contaminated aerosols. Lp can colonize premise plumbing systems due to favorable growth conditions (e.g., lower disinfectant residual, stagnation, warm temperatures). UV-C light-emitting diodes (UV-C LEDs) are an emerging water treatment technology and have been shown to effectively inactivate waterborne pathogens. In this study, the inactivation of four Lp strains (three clinical sg1, 4, and 6; and one sg1 drinking water (DW) isolate) was evaluated using a UV-C LED collimated beam at three wavelengths (255, 265, and 280 nm) and six fluence rates (0.5–34 mJ/cm2). Exposure to 255 nm resulted in higher log reductions at the lower fluences compared to exposures at 265 and 280 nm. Efficacy testing was also performed using a UV-C LED point-of-entry (POE) flow-through device. Based on the log inactivation curves, at 255 nm, the sg4 and sg6 clinical isolates were more susceptible to inactivation compared to the two sg1 isolates. However, at 265 and 280 nm, the sg1 and sg4 clinical isolates were more resistant to inactivation compared to the sg6 clinical and sg1 DW isolates. Differential log reductions were also observed using the POE device. Results indicate that although UV-C LED disinfection is effective, variations in Lp inactivation, wavelengths, and technology applications should be considered, especially when targeting specific isolates within premise plumbing systems.
Redetermination of the Zalantun Group in the ARong Qi Area of Da Hinggan Mountains (Northeastern China): Evidence from Petrology, Geochronology and Geochemistry
Authors: Wu Datian , Li Zhumin , Lv Junchao , Xu Jia , Shu Guanglong
Published: February 2022

The Da Hinggan Mountains are an important area in the tectonic evolution of the Central Asian Orogenic Belt (CAOB), and there are disputes over the closure time of the Paleo-Asian Ocean and the amalgamation spatiotemporal relationship between the Ergun-Hinggan Massif and the Songnun Massif. The geochronology and geochemistry of the Late Cambrian-Late Silurian volcanic rock assemblages in the ARong Qi area at the eastern margin of the Da Hinggan Mountains are studied in this paper. The results suggest that the U-Pb zircon ages of the Late Cambrian, Late Ordovician and Late Silurian volcanic rock assemblages are 507.5 ± 1.0 Ma, 456.2 ± 1.0 Ma, 446.1 ± 0.95 Ma and 423.3 ± 1.4 Ma, respectively. The Late Cambrian-Late Silurian volcanic rocks are quasi-aluminous-peraluminous, belonging to calc-alkaline-shoshonite series, which is rich in HREE but has insignificant europium anomalies. There are abundant large ion lithophile elements (LILE) in the rock, and remarkable Nb, Ta and Ti negative anomalies. The previous data and the current study indicate that a continental margin arc tectonic setting existed in the ARong Qi-Zalantun region during the Early Paleozoic, which is inferred to be the product of the subduction-accretion-amalgamation of the plates along the eastern margin of the Ergun Massif during the Early Paleozoic.
The Mechanism of a Retro-Diels–Alder Fragmentation of Luteolin: Theoretical Studies Supported by Electrospray Ionization Tandem Mass Spectrometry Results
Authors: Śliwka-Kaszyńska Magdalena , Anusiewicz Iwona , Skurski Piotr
Published: February 2022

The mechanisms of retro-Diels–Alder fragmentation of luteolin are studied theoretically using the Density Functional Theory method (B3LYP hybrid functional) together with the 6-311++G(d,p) basis set and supported by electrospray ionization tandem mass spectrometry (ESI-MS) results. The reaction paths leading to the formation of 1,3A− and 1,3B− fragment ions observed as the main spectral features in the ESI-MS spectrum are described and discussed, including the structures of the transition states and intermediate products. The heights of the activation energy barriers which have to be overcome along the reaction paths corresponding to 1,3-retrocyclization cleavage of the ionized luteolin are predicted to span the 69–94 kcal/mol range (depending on the initial isomeric structure) for the concerted retrocyclization mechanism and the 60–89 kcal/mol (first barrier) and 24–52 kcal/mol (second barrier) barriers for the stepwise mechanism (also depending on the initial isomeric structure). It is also demonstrated that the final fragmentation products (1,3A− and 1,3B−) are in fact represented by various isomeric systems which are not experimentally distinguishable. In addition, the absence of the spectral feature corresponding to the [M-B]− fragment ion formed by the rupture of the C-C bond connecting luteolin’s B and C rings (which does not occur during the ESI-MS experiment) is explained by much larger energy barriers predicted for such a process.
Unprecedented Epimerization of an Azithromycin Analogue: Synthesis, Structure and Biological Activity of 2′-Dehydroxy-5″-Epi-Azithromycin
Authors: Kragol Goran , Steadman Victoria A. , Marušić Ištuk Zorica , Čikoš Ana , Bosnar Martina , Jelić Dubravko , Ergović Gabrijela , Trzun Marija , Bošnjak Berislav , Bokulić Ana , Padovan Jasna , Glojnarić Ines , Eraković Haber Vesna
Published: February 2022

Certain macrolide antibiotics, azithromycin included, possess anti-inflammatory properties that are considered fundamental for their efficacy in the treatment of chronic inflammatory diseases, such as diffuse pan-bronchiolitis and cystic fibrosis. In this study, we disclose a novel azithromycin analog obtained via Barton–McCombie oxidation during which an unprecedented epimerization on the cladinose sugar occurs. Its structure was thoroughly investigated using NMR spectroscopy and compared to the natural epimer, revealing how the change in configuration of one single stereocenter (out of 16) profoundly diminished the antimicrobial activity through spatial manipulation of ribosome binding epitopes. At the same time, the anti-inflammatory properties of parent macrolide were retained, as demonstrated by inhibition of LPS- and cigarette-smoke-induced pulmonary inflammation. Not surprisingly, the compound has promising developable properties including good oral bioavailability and a half-life that supports once-daily dosing. This novel anti-inflammatory candidate has significant potential to fill the gap in existing anti-inflammatory agents and broaden treatment possibilities.
Comparative analysis of some parameters of social and dental statuses, behavioral habits of patients presented to a dentist for different reasons
Authors: Aliamovskii V. V. , Duzh A. N.
Published: February 2022

Relevance. The health of the population is an essential criterion of the well-being of society. The high prevalence of dental diseases, which reduce the quality of life, dictates a broader implementation of preventive measures. Correcting the regularity of oral hygiene and consumption of fast digesting carbohydrates can achieve significant results. Purpose – to study some parameters of socio-hygienic, dental statuses and habits of patients who presented to the Health Center.Materials and methods. To achieve the purpose, we interviewed and examined 550 people: 196 men and 354 women aged 18-85 who presented to the Health Center of Krasnoyarsk Interdistrict Hospital No. 3. The study analysed the structure of patients' visits to dentists and determined dental caries prevalence and intensity. The following definition was chosen to assess the behavioral habits of patients: the frequency of oral care during the day, the frequency of consuming fast digesting carbohydrates, the rate of toothbrush replacement. Statistical data was processed using the IBM SPSS Statistics 18.0 software package.Results. 9.45% of patients exhibited low motivation for dental check-ups, most pronounced at a young age – 30,02 ± 2,00 years. The best motivation to visit a dentist for a dental check-up was among young people with higher or incomplete higher educa-tion – 57,7%. Visits to dentists for unspecified reasons were most pronounced in patients with primary or secondary education – 63,8%. The patients who presented for a dental check-up and prophylaxis exhibited the lowest caries intensity rate – 9,27 ± 1,01, and there were statistically significant differences in the DMF index depending on the education of the respondents (p = 0,016), including the components of the "F" and "M" (p < 0,001). Patients, who come for regular dental check-ups, have better oral hygiene, but very few of them limit the consumption of fast digesting carbohydrates.Conclusions. The patient motivation for dental check-ups is extremely low and depends on age and education. Patients, who visit dentists for a check-up, have the lowest intensity of dental caries and statistically significant differences in the components of the DMF index depending on education.The establishing of health centres in the domestic health care system to promote a healthy lifestyle in Russian citizens, including smoking and alcohol consumption reduction, allows using their organisational, methodological and material and technical capabilities for further studying the issues of dental disease prevention, creating the most balanced questionnaires and other tools for assessing and monitoring dental health.
Prevalence of malocclusion and orthodontic treatment needs among 15-year-old adolescents of the Arkhangelsk region
Authors: Simakova A. A. , Gorbatova M. A. , Grjibovski A. M. , Gorbatova L. N.
Published: February 2022

Relevance. Purpose of the study to assess the prevalence of malocclusion and orthodontic treatment needs among 15-year-old adolescents of the Arkhangelsk region.Materials and methods. This cross-sectional study is a part of the Third National dental examination of children and adolescents of the Russian Federation. The sample consisted of 1091 teenagers from 7 urban and 5 rural settlements. The study assessed the prevalence of malocclusion and calculated the Dental Aesthetic Index (DAI) to evaluate orthodontic treatment needs according to the sex and place of residence. The prevalence of malocclusion was presented by proportions with 95 % confidence intervals (CI) calculated by the Wilson method. Categorical variables were compared using Pearson’s chi-squared test. The numeric variables were analyzed by the Mann-Whitney test. Results. The malocclusion prevalence was 67% (95% CI: 64,2–69,8) without sex or location of residence differences. The mean DAI score was 24,0 (95% CI: 23,2–24,8) for the rural and 22,9 (22,5–23,4) for the urban adolescents (p < 0,001). 33,5 % of the 15-year-olds needed orthodontic treatment, with no differences in sex or place of residence. 18,7% of adolescents exhibited overt malocclusion, 9,7% had severe malocclusion, and 5,0% – very severe.Conclusions. The prevalence of malocclusion and orthodontic treatment needs in the Arkhangelsk region remain high. The mean DAI score was higher in rural than in urban adolescents. All the above mentioned indicates the necessity to prevent and timely treat orthodontic pathology in adolescents.
TINJAUAN KEGIATAN PEMBERANTASAN SARANG NYAMUK (PSN) DAN INDEKS AEDES AEGYPTI INVESTATION LEVEL DI KELURAHAN PADANG SAMBIAN KAJA KECAMATAN DENPASAR BARAT TAHUN 2017
Authors: Rahma Primantari Ni Luh Ayu , Suyasa I Nyoman Gede
Published: February 2022

Abstract:The high cases of DHF are strongly influenced by environmental factors that include geographical and demographic conditions. These factors are very influential on the population density of Aedes Aegypti mosquitoes. The presence of larva Aedes Aegypti in a region is an indicator of the presence of Aedes Aegypti mosquito populations in the area so that it can lead to the occurrence of cases of DHF, therefore the eradication strategy needs to be done through mosquitoes eradication activities (PSN) by the community continuously and periodically. The purpose of this research is to know the review of Mosquito Nest (PSN) and Aedes aegypti investation level in Padang Sambian Kaja Subdistrict, West Denpasar District. The type of descriptive study with cross-sectional design. The number of samples is 97 people with random sampling method. Data collection by doing larvae and kusioner examination. Result of research got result of implementation of eradication of mosquito nest by society in sub district of Padang Sambian Kaja District of West Denpasar mostly in less category that is counted 41 person (42,3%). The index of Aedes Aegypti Infestation Level record in Padang Sambian Kaja Village is included in the high risk of HI = 82.97%, CI = 19.61%, and BI = 156.70%. Suggestion to involve the community in larva examination by forming cadres jumantik so it can perform periodic larvae examination at each region.