bluuebunny/crossref_metadata_2025 huggingface dataset:
Le concept de la biotenségrité et son utilisation en ostéopathie
Authors: Tarento M.
Published: July 2019

Les termes de tenségrité et de biotenségrité sont utilisés de plus en plus souvent dans les articles et dans les cours d′ostéopathie du monde entier; mais le plus souvent les commentaires qui les précèdent ou les accompagnent montrent que les principes et les concepts qu′ils recouvrent ne sont qu′effl eurés et, bien souvent, peu ou mal compris. Or, les principes des constructions en tenségrité s′appliquent de manière étonnamment adaptée à la compréhension de «comment», en ostéopathie, nous questionnons et réajustons le réseau fascial du corps humain et ce, quel que soit la technique utilisée. En effet le réseau fascial associe des éléments solides en discontinuité et des éléments souples qui créent la continuité et forment une globalité dont tous les éléments réagissent et se réorganisent sous la contrainte. La tension et la compression qui se déploient simultanément dans des directions opposées de la trame fasciale se répartissent depuis l′échelle macroscopique jusqu′à l′échelle microscopique des ensembles Ostéo Myo Fasciaux du corps ; une des conséquences importantes de cette réorganisation étant de préserver l′homéostasie. Ainsi, entrer en relation par le toucher avec le dynamisme tenso-compressionnel ainsi qu′avec l′aspect vibratoire des éléments du vivant selon les modélisations géométriques et les principes architecturaux proposés par la tenségrité, permettra une meilleure compréhension et un accompagnement très abouti de nos gestes ostéopathiques.
Possibilities of osteopathic correction in complex therapy of temporomandibular joint dysfunction
Authors: Milutka Yu. A. , Yushmanov I. G. , Badmaeva A. N.
Published: July 2019

Introduction.Dysfunction of the temporo-mandibular joint is a frequent, complex, multifactorial disease that is traditionally treated by orthodontic methods, but not always with a positive result.

Goal of research— to assess the effect of osteopathic treatment combined with orthodontic treatment on the temporo-mandibular joint dysfunction and osteopathic status of patients with TMJ disfunction.

Materials and methods.The study included 40 patients with the temporo- mandibular joint dysfunction receiving orthodontic treatment (hard and soft splints, brace system, exercises). All patients were women without severe somatic pathology divided into 2 groups: treatment group and control group. The mean (±SD) age of participants was 27,5±5,3 and 29,2±5,7 years, respectively. In both groups, osteopathic, orthodontic status have been evaluated, patients completed a questionnaire on the status of the temporo-mandibular joint. In the main group, the described orthodontic methods of treatment were supplemented by osteopathic correction.

Results.The study showed a decrease in the number of somatic dysfunctions, objective symptoms and subjective sensations arising from dysfunction of the temporo-mandibular joint. The analysis showed a greater effi cacy of combined osteopathic and orthodontic treatment in comparison with exclusively orthodontic treatment.

Conclusion.The obtained results provide possibility of further research of the effectiveness of osteopathic correction in combination with orthodontic treatment of the temporomandibular joint dysfunction.
Сhange in the functional activity of fibroblasts in the process of modelling of compression, hypercapnia and hypoxia
Authors: Aptekar A. I. , Kostolomova E. G. , Sukhovey Y. G.
Published: July 2019

Introduction.In the genesis of the formation of somatic dysfunction, the leading role belongs to the reactions of the organism in the whole and of the connective tissue in particular. The main connective tissue cells are fibroblasts, possessing the full basic properties of cells.

Goal of research— to examine changes in the functional activity of fibroblasts in the process of modelling of compression, hypercapnia and hypoxia.

Materials and methods.During the experiment (in vitro), simulation of compression, hypercapnia and hypoxia in human fibroblast culture was carried out.

Results.In response to simulated changes in environmental conditions, fibroblasts changed their local habitat (intercellular substance) by changing the balance of elastin and collagen, and adapted morphologically (by changing their shape). Compression and hypercapnia had the most damaging effect on the main cells of the connective tissue

Conclusion.Determining of the dependence of the reaction of fibroblasts on damaging effects can form the basis for justifying the sequence of the use of methods aimed to eliminate connective tissue disorders in the process of osteopathic correction (decompression, reduction of edema and hypoxia).
Impact of Hand Dominance on Effectiveness of Chest Compressions in a Simulated Setting: A Randomised, Crossover Trial
Authors: Cross Jamie , Lam Tommy , Arndell Joel , Quach John , Reed Buck , Thyer Liz , Simpson Paul M
Published: July 2019

Aim

External cardiac compressions (ECC) are a critical component in determining the effectiveness of cardiopulmonary resuscitation (CPR). Guidelines prior to the 2010 International Liaison Committee on Resuscitation directed rescuers to place the heel of the dominant hand directly on the chest when performing ECC, however current guidelines are silent on this issue. Existing research is inconsistent in findings, and heterogeneous in design and participants. The aims of this pilot study were to: 1) investigate the impact of hand dominance on effectiveness of ECC; and 2) generate outcome data to inform sample size calculations for a larger future study.

Methods

This study utilised a single blinded, prospective randomised crossover trial design. Each participant was allocated to a ‘dominant hand on chest’ (DHOC) or ‘non-dominant hand on chest’ (NDHOC) group. On a simulation manikin, participants in the DHOC group performed 3 minutes of ECC with dominant hand on the chest and non-dominant hand supporting, followed by a ‘rest and recovery’ period and then a second 3-minute period of ECC with the hand reversed such that the non-dominant hand was on the chest. The NDHOC group performed the same series of compressions but in reverse order. The primary outcome measure was effectiveness of ECC, determined by a percentage-based ‘CPR score’ (‘CS’). Secondary outcomes were compression depth, rate and release. The Wilcoxon rank-sum (Mann-Whitney) test was used due to the non-normal distribution of the data. Due to the crossover design, hierarchical linear regression was used to assess for a period or cross over effect.

Results

For the primary outcome of this study, we have found no significant difference in CS between DHOC and NDHOC (69.9% (SD=29.9) vs. 69.1% (SD=34.1); p=0.92), respectively. There were no differences in the secondary outcomes of compression rate and depth, though compression release was improved in the DHOC group (53% vs. 42%; p=0.02).

Conclusion

In this randomised crossover study conducted in a simulation context there was no difference in ECC effectiveness measured by an overall effectiveness outcome according to placement of the dominant or non-dominant hand on the chest during compressions. A modest improvement in ECC release was seen in the dominant hand on chest group. While the study was underpowered, the results support an approach involving rescuers placing whichever hand they are most comfortable with on the chest irrespective of handedness.
MULTICULTURALISM IN ETHNIC IN CHILDREN'S POPULAR FILM IN INDONESIA POST 2010
Authors: Rozi Romdhi Fatkhur
Published: July 2019

This article discusses about multiculturalism in the popular film industry with the theme of children in Indonesia after 2010. The categorization of this popular film is based on the number of viewers. This study is intended to find many aspects of ethnic multiculturalism contained in child-theme films after 2010. The concept of children's films from Bezalgette and Stapless is used to review the children's theme films. The conclusions of this study are that multiculturalism is present in most children's films in Indonesia through various forms of moral messages, which are delivered from interactions between characters and characterizations, however the films have not succeeded in showing all multi-ethnic diversity in Indonesia yet.
Internações hospitalares por dependência quí­mica no Brasil: um estudo epidemiológico
Authors: Araújo Raiane Jordan da Silva , Sobral Janaí­na Paula Calheiros Pereira , De Souza Roberta Virtuoso , De Souza Yasmin Virtuoso
Published: July 2019

EEsta pesquisa teve como objetivo analisar os dados epidemiológicos acerca das internações hospitalares por transtornos mentais e comportamentais decorrentes do abuso do álcool e de outras substâncias psicoativas no Brasil, de 2007 a 2016. Configura-se uma pesquisa descritiva de corte transversal com abordagem quantitativa baseada em dados documentais oriundos do Sistema de Informações Hospitalares/DATASUS. Os percentuais analisados e discutidos foram: ano, sexo, idade e região. Houve redução de mais de 30% na quantidade de internações no perí­odo de 2011 a 2016, com predominância no sexo masculino, faixa etária de 30 a 49 anos, em São Paulo e Região Sudeste. O presente estudo constatou que houve impacto positivo na saúde pública no sentido da desinstitucionalização nos tratamentos em saúde mental e que o público feminino e a região sudeste precisam ser alvos de mais ações em relação ao uso do álcool e de outras substâncias psicoativas e a redução de danos.Â Palavras-chave: transtornos relacionados ao uso de substâncias, hospitalização, saúde mental.Â
A percepção de cuidadoras sobre os cuidados com a criança soropositiva ao HIV
Authors: Ferreira Samuel Lima , De Sousa Isabelle Vasconcelos , Fernandes Marcos Viní­cius Costa , Esteves Arinete Véras Fontes , Rocha Ellen Pessoa
Published: July 2019

Introdução: A soropositividade implica em desafios para seus portadores, principalmente na infância, pois repercute negativamente na construção interpessoal e no desenvolvimento biopsicossocial da criança. O ser cuidador/orientador atua como auxiliar na transição entre a infância e adolescência, de modo a buscar novas formas para melhorar a qualidade na assistência desses menores soropositivos. Objetivos: Compreender a percepção do cuidador quanto í  rotina de cuidados com a criança soropositiva e a aceitação quanto a construção de uma tecnologia educacional para este contexto, bem como conhecer a rotina e as dificuldades enfrentadas pelas cuidadoras durante a assistência. Métodos: Estudo descritivo exploratório com abordagem qualitativa, realizado na Associação de Apoio í  Criança com HIV (AACH) localizada em Manaus. Os dados foram coletados por meio de entrevista semiestruturada com 8 cuidadoras da instituição e foram analisados conforme a técnica de análise qualitativa de dados. Resultados: Os resultados mostram aspectos importantes a serem considerados durante a realização dos cuidados a criança soropositiva, dentre os quais o medo de infectar-se, a dificuldade de revelar o diagnóstico e a carência de novas metodologias para o cuidar mostram-se como as principais peculiaridades durante o assistir ao menor soropositivo. Conclusão: Nota-se, assim, a necessidade de implementação de tecnologias educativas pautadas para a educação das cuidadoras e crianças com HIV.Palavras-chave: sorodiagnóstico da AIDS, criança, cuidados, educação em saúde.
Significado da histerectomia para a mulher e suas implicações na assistência de enfermagem
Authors: Salimena Anna Maria Oliveira , Ribeiro Marcela Oliveira Souza
Published: July 2019

Objetivou-se refletir sobre o significado da histerectomia para as mulheres e suas implicações na assistência de enfermagem no pré-operatório. Utilizou-se como metodologia de estudo a revisão/reflexão sobre a temática. Foi possí­vel observar que o pré-operatório de histerectomia é vivenciado de maneira singular para cada mulher, representando para algumas um momento doloroso de perda de um órgão repleto de simbolismos em relação ao universo feminino, enquanto que, para outras, é um sentimento de alí­vio frente ao cessar dos sintomas que levaram í  necessidade cirúrgica. Compreendeu-se que a enfermagem tem papel fundamental durante todo o perioperatório, principalmente no perí­odo que antecede a cirurgia. Recorre-se í  visita pré-operatória como uma oportunidade de escuta e orientações, a partir da identificação das necessidades especí­ficas de cada mulher, promovendo acolhimento e minimizando desconfortos contemplando a Sistematização da Assistência de Enfermagem Perioperatória.Palavras-chave: saúde da mulher, histerectomia, assistência de enfermagem.
Autocuidado em hansení­ase: comportamento de usuários atendidos na rede de atenção primária í saúde
Authors: Carvalho Paula Soares , De Brito Karen Krystine Gonçalves , De Santana Emanuelle Malzac Freire , De Lima Siméia Macêdo , Andrade Smalyanna Sgren da Costa , Nóbrega Matheus de Medeiros , Soares Maria Júlia Guimarães Oliveira
Published: July 2019

Introdução: A hansení­ase é uma doença negligenciada e incapacitante de relevância para a saúde pública brasileira. O autocuidado se configura como estratégia de prevenção contra suas incapacidades fí­sicas. Objetivo: Investigar as competências dos indiví­duos com hansení­ase atendidos na atenção primária í  saúde, direcionado a ações de autocuidado através da escala Appraisal of Self-care Agency. Métodos: Pesquisa quantitativa realizada nas unidades de saúde da famí­lia em João Pessoa/PB, com 22 participantes de janeiro a março de 2017. Utilizou-se na coleta de dados um instrumento sociodemográfico e a escala Appraisal of Self-care Agency. Os dados foram analisados por técnicas de estatí­stica descritiva e inferencial através dos testes U de Mann-Whitney e Kruskal Wallis. Resultados: Perfil caracterizado por maioria de mulheres, com média de 52 anos, tipo multibacilar, grau de incapacidade não especificado, com média de 92 pontos na escala ASA. Conclusão: Observou-se em uma taxa majoritária dos entrevistados a competência para o autocuidado.Palavras-chave: hansení­ase, atenção primária í  saúde, autocuidado, Enfermagem.
Letramento em saúde dos comunicantes familiares de pacientes com tuberculose pulmonar
Authors: Da Silva Marilena Pinheiro Souza , Botelho Eliã Pinheiro , De Carvalho Jacira Nunes , Gonçalves Lucia Hisako Takase
Published: July 2019

Objetivo: Identificar o ní­vel de letramento em saúde (LS) dos comunicantes de pacientes com tuberculose pulmonar. Métodos: Estudo qualitativo-descritivo, realizado no ambulatório de um Hospital Universitário. Participaram 30 comunicantes selecionados por conveniência cujos dados foram coletados aplicando-se o instrumento "Alfabetização em Saúde". Dados analisados segundo dimensões do construto letramento. Resultados: Baixo ní­vel socioeconômico e de escolaridade refletiram sobre o limitado ní­vel de letramento em saúde dos comunicantes familiares. A postura de omissão ou silêncio sobre a tuberculose e sobre busca e compartilhamento de informações parece ser influenciada pelo preconceito em torno da doença que ainda impera entre as pessoas que não se beneficiam dos conhecimentos e práticas de prevenção da contaminação pela tuberculose pulmonar. Conclusão: De baixo ní­vel de LS detectado, deduz-se que os comunicantes de pacientes tuberculosos correm risco de contaminarem-se como também os demais familiares que costumam ter conví­vio mais í­ntimo e duradouro. Intervenções cuidativo-educacionais por profissionais, especialmente enfermeiros, são essenciais na prevenção de contaminação e de disseminação da doença, junto ao núcleo familiar do paciente tuberculoso.Palavras-chave: tuberculose pulmonar, comunicante familiar, famí­lia, Enfermagem.
Cognição prejudicada de idosos em instituições de longa permanência pública de São Paulo
Authors: Scherrer Júnior Gerson , Simão Marineusa , Passos Kleyton Góes , Ernandes Rita de Cássia , Alonso Angélica Castilho , Belasco Angélica Gonçalves da Silva
Published: July 2019

Introdução: O envelhecimento populacional desencadeia perdas na capacidade funcional, entre elas as cognitivas, que merecem atenção por comprometer a qualidade de vida dos idosos institucionalizados. Objetivo: Caracterizar o perfil dos idosos residentes em instituições de longa permanência pública de São Paulo com perda cognitiva. Métodos: Estudo quantitativo, transversal e descritivo, com 154 idosos residentes em quatro instituições nas zonas norte, sul, leste e oeste da cidade de São Paulo. As instituições estão inseridas no seio da comunidade e acolhem idosos em situação de vulnerabilidade social. A coleta aconteceu durante os meses de agosto a dezembro de 2016. As informações sociais, culturais e epidemiológicas foram transcritas dos prontuários, também avaliado o estado mental, o grau de dependência e o risco de desnutrição. Resultados: 62,4% apresentavam cognição prejudicada, desses prevaleceu o sexo feminino 36,4%, a faixa etária de 70 a 79 anos 28%, solteiros 35,8% e analfabetos 28%, sem queda 46,1%, entre dois a quatro anos de institucionalização 23,5%, totalmente dependentes 29,9% e risco para desnutrição 54%. Conclusão: Os idosos com perda cognitiva são do sexo feminino, com idade avançada, solteiros ou viúvos, com baixa escolaridade, sem queda, entre dois a quatro anos de institucionalização, com dependência e risco de desnutrição.Palavras-chave: instituição de longa permanência para idosos, cognição, Enfermagem.
The implementation of total quality management in vocational high schools
Authors: Rahayuningsih Retna Sundari , Fajaruddin Syarief , Manggalasari Lena Citra
Published: July 2019

The study aimed to find out the implementation of teachers’ focus on students and the level of students’ satisfaction, the implementation of the involvement of school’s communities, the implementation of teacher service measurement, the implementation of the commitment of school’s communities, and implementation of teacher services improvement. This particular evaluative research used descriptive-quantitative design. The evaluation model was a discrepancy evaluation model. The research sites were Negeri 2 Pengasih State Vocational High School, Negeri 1 Nanggulan Kulon Program State Vocational High School, Ma’arif 1 Wates Kulon Progo Vocational High School and Muhammadiyah 1 Temon Kulon Progo Vocational High School. The subjects of this study were students, teachers, and principals from four schools. The objects of this research were: (1) the implementation of teachers’ focus on students, (2) the implementation of the involvement of school’s communities, (3) the implementation of teacher service measurement, (4) the implementation of the commitment of school’s communities, and (5) the implementation of teacher service improvement. Data collection techniques were observation, document analysis, and questionnaire. Data collection instruments used observation rubric, document analysis sheets, and questionnaire. The level of implementation reported in a percentage which reports a comparison between the averages score and the maximum score. The results of this study showed that the overall average in four vocational high schools were: (1) the level of teacher's focus on students was 81.13% (very good), and the level of students’ satisfaction was 77.69% (students feel satisfied); (2) the level of involvement of school’s communities was 80.28% (good); (3) the level of teacher service measurement was 77.50% (good); (4) the level of the commitment of school’s communities was 79.77% (good); and (5) the level of teacher service improvement was 77.50% (good).Keywords: total quality management, vocational high school
Vicente Llorente y Matos : un microbiólogo en las Cortes de la Restauración (1899-1905)
Authors: Mateo de la Hoz Mónica , Granda Vega Enrique
Published: July 2019

1.- Breve reseña sobre la legislación sanitaria a finales del siglo XIX y principios del XX. 2.- Otros elementos a considerar para la entrada en política del Dr. Llorente 3.- La situación política de Canarias en el cambio de siglo. 4.- Participación política. 4.1. Legislaturas 1899-1900 y 1900-1901. 4.2. Legislatura 1903-1904. 4.3. Legislatura 1904-1905. 5.- Conclusión. 6.- Bibliografía.
El principio de publicidad parlamentaria en las Cortes de Cádiz
Authors: Fernández Segado Francisco
Published: July 2019

SUMARIO: 1.- Algunas reflexiones generales sobre el nacimiento del principio de publicidad parlamentaria: A) El secreto de los debates y votaciones como parte integrante de los privileges of Parliament en Inglaterra. Su progresivo desuso. B) La publicidad como principio de mediación con vistas a la dirección de la colectividad a través del empleo de la razón. C) La vigorosa y rotunda defensa de la publicidad parlamentaria llevada a cabo por Jeremy Bentham.  D) La constitucionalización del principio de publicidad parlamentaria. E) Los benéficos efectos del principio de publicidad parlamentaria. El principio burkeano del gobierno y la legislación como asuntos de razón y juicio (matters of reason and judgment) y no de voluntad (matter of will). 2.- La publicidad de las sesiones parlamentarias en las Cortes de Cádiz: A) Tacticismo político o mera afirmación de un principio inherente a las señas de identidad del ideario liberal?. B) Las sucesivas regulaciones de la publicidad de las sesiones. 3.- El contrapunto de las sesiones secretas. 4.- La presencia del público en las tribunas: A) Su comportamiento. Los frecuentes alborotos y desórdenes. B) El grave incidente del 26 de octubre de 1811 en el que se vio afectado el diputado José Pablo Valiente. 5.- El periódico de las Cortes.
Informe del Letrado sobre el Proyecto de Ley Orgánica para la mejora de la calidad educativa (621/000048)
Authors: García Mexía Pablo
Published: July 2019

I.- Introducción. II.- Orientación General del Proyecto. II.1. Ordenamiento actualmente en vigor. II.2. Objetivos. III.- Estructura del Proyecto. IV.- Tramitación anterior a la entrada en el Senado. IV.1. Fase de anteproyecto. IV.2. Tramitación del proyecto en el Congreso de los Diputados. IV.2.A. Fase de Ponencia. IV.2.B. Fase en Comisión. IV.2.C. Fase en Pleno. V.- Análisis del contenido. V.1. Consideraciones jurídicas. V.2. Consideraciones de técnica legislativa. V.3. Consideraciones terminológicas y gramaticales. [Anexo. (1) Texto consolidado de la ley orgánica 2/2006, de 3 de mayo, de educación (LOE) tras la incorporación del texto LOMCE aprobado por el Congreso de los Diputados.]
Reflexiones sobre algunas propuestas de reforma de los sistemas electorales autonómicos : de la modificación del tamaño de la Cámara a la aplicación del sistema alemán
Authors: Uribe Otalora Ainhoa
Published: July 2019

El artículo analiza las propuestas de reforma de los sistemas electorales autonómicos abanderadas por el Partido Popular en algunas Comunidades Autónomas, entre 2011 y 2015, que pretendían paliar la desafección ciudadana, mediante la reducción del número de los diputados autonómicos e, incluso, mediante la introducción de distritos uninominales para elegir una parte de los diputados. El estudio analiza cómo hacer posible la combinación de los distritos uninominales con las circunscripciones provinciales, en el marco de la Constitución, así como se estudian otras cuestiones de interés en la reforma (tamaño de la cámara, tipo de fórmula electoral, barrera, etc.). Para ello, se exploran dos alternativas de reforma electoral que se podrían llevar a cabo en las Comunidades Autónomas, ahora o en un futuro: 1) la aplicación del sistema electoral alemán; y 2) la adopción del voto único transferible (empleado en Malta y en Irlanda). En suma, el artículo desgrana los pros y los contras de las medidas propuestas por el PP, desde la perspectiva del objetivo que se persigue y atendiendo a las dificultades jurídicas y políticas de una posible reforma electoral autonómica.
Estudio sobre el grado de reconocimiento de la nación y de las nacionalidades en la Constitución española
Authors: Del Real Alcalá Julio Alberto
Published: July 2019

1. Introducción: el reconocimiento legal de la nación y de las nacionalidades. 2. La concreción normativa de la nación española en la Constitución de 1978. 3. La vaguedad normativa de las nacionalidades en la Constitución Española. 4. Conclusión.
El papel del Consejo Europeo en la construcción europea y en la actualidad
Authors: Calatrava Olga
Published: July 2019

Artículo sobre el papel del Consejo Europeo en la construcción europea y en la actualidad
La reforma del Reglamento del Congreso de los Diputados: una asignatura histórica
Authors: Pérez Alamillo Ricardo
Published: July 2019

Notas sobre la reforma del Reglamento del Congreso de los Diputados
Pablo Santolaya Machetti : bibliografía e intervenciones en el Parlamento
Authors: Buñuel Adán Marta
Published: July 2019

Obra sobre Pablo Santolaya Machetti y sus intervenciones en el Parlamento
Claro José Fernández-Carnicero : la inteligencia y la palabra
Authors: Jiménez Díaz Andrés
Published: July 2019

Notas sobre Claro José Fernández-Carnicero
Gene Expression in Patients With Abdominal Aortic Aneurysm – More Than Immunological Mechanisms Involved
Authors: PRUCHA M , SEDIVY P , STADLER P , ZDRAHAL P , MATOSKA V , STRNAD H
Published: July 2019

Abdominal aortic aneurysm (AAA) is a serious condition of unclear pathogenesis and progression. Two samples were collected from 48 patients during AAA surgery. One sample was collected from the aneurysm, the other from the aneurysm proximal neck where the tissue did not exhibit any aneurysmal changes. Subsequently, gene expression profiles using microarrays (Illumina) were compared in RNA extracted from the samples. Overall, 2,185 genes were found to be upregulated and 2,100 downregulated; from which 158 genes had a different expression with FDR<0.05 (False Discovery Rate) and FC≥2 (Fold Change). Of this number, 115 genes were over-expressed and 43 under-expressed. The analysis of the gene list based on their biological pathways revealed that the regulation of inflammation was mediated by chemokine and cytokine signaling pathways, the integrin signaling pathway, and T and B cell activation. Moreover, a change was identified in the expression of genes involved in both intercellular and intracellular signaling systems.
Various AKIP1 Expression Levels Affect Its Subcellular Localization but Have no Effect on NF-κB Activation
Authors: KEPROVÁ A. , KOŘÍNKOVÁ L. , KŘÍŽOVÁ I. , HADRAVOVÁ R. , KAUFMAN F. , PICHOVÁ I. , RUML T. , RUMLOVÁ M.
Published: July 2019

A-kinase interacting protein 1 (AKIP1) has been shown to interact with a broad range of proteins involved in various cellular processes, including apoptosis, tumorigenesis, and oxidative stress suggesting it might have multiple cellular functions. In this study, we used an epitope-tagged AKIP1 and by combination of immunochemical approaches, microscopic methods and reporter assays we studied its properties. Here, we show that various levels of AKIP1 overexpression in HEK-293 cells affected not only its subcellular localization but also resulted in aggregation. While highly expressed AKIP1 accumulated in electron-dense aggregates both in the nucleus and cytosol, low expression of AKIP1 resulted in its localization within the nucleus as a free, non-aggregated protein. Even though AKIP1 was shown to interact with p65 subunit of NF-κB and activate this transcription factor, we did not observe any effect on NF-κB activation regardless of various AKIP1 expression level.
Hydrogen Sulfide Plays an Important Protective Role by Influencing Autophagy in Diseases
Authors: WANG J. , WU D. , WANG H.
Published: July 2019

Autophagy can regulate cell growth, proliferation, and stability of cell environment. Its dysfunction can be involved in a variety of diseases. Hydrogen sulfide (H2S) is an important signaling molecule that regulates many physiological and pathological processes. Recent studies indicate that H2S plays an important protective role in many diseases through influencing autophagy, but its mechanism is not fully understood. This article reviewed the progress about the effect of H2S on autophagy in diseases in recent years in order to provide theoretical basis for the further research on the interaction of H2S and autophagy and the mechanisms involved.
Correlation of Tumor Necrosis Factor Alpha, Interleukin 6 and Interleukin 10 With Blood Pressure, Risk of Preeclampsia and Low Birth Weight in Gestational Diabetes
Authors: ŽÁK P. , SOUČEK M.
Published: July 2019

Several pathophysiological mechanisms have been proposed in the development of pregnancy complications, including endothelial dysfunction, an inflammatory pathway and oxidative stress. The aim of the present study was to evaluate the correlation between proinflammatory cytokines TNF-α, IL-6 and dual cytokine IL-10 in the mother’s peripheral blood and systolic blood pressure, risk of preeclampsia and low birth weight in gestational diabetes (GDM). We observed 40 women with GDM divided into a gestational hypertension group (n=20) and comparison group (n=20) with normal blood pressure. We found a significant positive correlation between TNF-α; IL-6; IL-10 levels and systolic blood pressure (SBP) in the second trimester (p<0.001; p<0.001; p<0.001); the third trimester (p<0.001; p<0.001; p<0.05). We also proved correlations for diastolic blood pressure (DBP) during the second; third trimester (p<0.001; p<0.001; p<0.001); (p<0.001; p<0.001; p<0.0015). We demonstrated a statistically significant positive association between high TNF-α group and preeclampsia risk in the third trimester (p=0.04). We also determined the negative correlation in the second trimester between birth weight and TNF-α; IL-6, IL-10 levels (p<0.05; p<0.001; p<0.001). To conclude, our data highlight the importance of cytokines TNF-α, IL-6 and IL-10 in blood pressure regulation. In addition, high levels of TNF-α have been associated with increased risk of preeclampsia. We found a significant negative correlation between levels of TNF-α, IL-6, IL-10 and birth weight.
Relationship Between Steroid Hormones and Metabolic Profile in Women With Polycystic Ovary Syndrome
Authors: LAZÚROVÁ I. , LAZÚROVÁ Z. , FIGUROVÁ J. , UJHÁZI S. , DRAVECKÁ I. , MAŠLANKOVÁ J. , MAREKOVÁ M.
Published: July 2019

Polycystic ovary syndrome (PCOS) is commonly associated with a higher cardiometabolic risk. The relationship between steroid hormones and cardiometabolic profile in PCOS has been evaluated, but no single hormonal predictor of this association has been identified to determine. To determine the relationship between steroid hormones and cardiometabolic risk factors in PCOS women. Study included 64 women diagnosed with PCOS. Fasting blood samples were analyzed for biochemical, metabolic parameters and sex steroid hormones. PCOS women with BMI≥27 had significantly higher serum free testosterone (FT), free androgen index (FAI), estrone (E1) (p=0.014, p=0.02, p=0.01) than those with normal weight. In all subjects E1 positively correlated with BMI (p=0.0067), serum insulin (p=0.0046), HOMA-IR (p=0.0125) and negatively with HDL-cholesterol (p=0.009). FAI positively correlated with serum cholesterol (p=0.0457), triacylglycerols (TAG) (p=0.0001), HOMA-IR (p=0.037), and glycemia (p=0.0001), negatively with HDL-cholesterol (p=0.029). In multiple linear regression model E1 most significantly predicted HOMA-IR, whereas FT/FAI predicted HDL-cholesterol and BMI. We conclude that PCOS women with marked overweight or obesity have higher FT, FAI and E1 as compared with nonobese PCOS subjects. E1 and FT may predict worse cardiometabolic profile in PCOS.
Transient Increase in Cellular Dehydrogenase Activity After Cadmium Treatment Precedes Enhanced Production of Reactive Oxygen Species in Human Proximal Tubular Kidney Cells
Authors: HANDL J. , ČAPEK J. , MAJTNEROVÁ P. , PETIRA F. , HAUSCHKE M. , ROUŠAROVÁ E. , ROUŠAR T.
Published: July 2019

Cadmium is a heavy metal causing toxicity especially in kidney cells. The toxicity is linked also with enhanced oxidative stress leading to cell death. On the other hand, our recent experiments have shown that an increase of total intracellular dehydrogenases activity can also occur in kidney cells before declining until cell death. The aim of the present study, therefore, was to evaluate this transient enhancement in cell viability after cadmium treatment. The human kidney HK-2 cell line was treated with CdCl2 at concentrations 0-200 µM for 2-24 h and intracellular dehydrogenase activity was tested. In addition, we measured reactive oxygen species (ROS) production, glutathione levels, mitochondrial membrane potential, and C-Jun-N-terminal kinase (JNK) activation. We found that significantly increased dehydrogenase activity could occur in cells treated with 25, 100, and 200 µM CdCl2. Moreover, the results showed an increase in ROS production linked with JNK activation following the enhancement of dehydrogenase activity. Other tests detected no relationship with the increased in intracellular dehydrogenase activity. Hence, the transient increase in dehydrogenase activity in HK-2 cells preceded the enhancement of ROS production and our finding provides new evidence in cadmium kidney toxicity.
Dynamic Rabbit Model of Ear Barotrauma
Authors: Wang Binru , Xu Xianrong , Lin Jianhong , Jin Zhanguo
Published: July 2019

BACKGROUND: Establishing animal models of ear barotrauma (EB) to provide evaluation criteria for Eustachian tube dysfunction.METHODS: Using expansive sponges, 70 rabbits’ right pharyngeal openings of the auditory tubes were blocked to cause dysfunction in the
right Eustachian tubes. The right tympanic cavities of 65 rabbits were the Model Group (Subgroups 1–13) and these rabbits’ left tympanic cavities were the Nonblockage Group. Hypobaric chamber tests (HCTs) at various vertical speeds (100 m · s−1, 75 m ·
s−1, 50 m · s−1, and 15 m · s−1) and altitudes (13,123 ft and 6562 ft) were conducted. The remaining five rabbits’ right tympanic cavities were the Control Group and no HCTs were conducted. After HCTs, observations were
made on rabbits’ behavioral changes, oto-endoscope and tympanometry results, and pathological changes of the tympanic mucosae.RESULTS: 1) Rabbits in Subgroups 1–12 demonstrated EB, while Subgroup 13 and the Control Group did not. 2) Histopathology showed EB caused by
rapid ascent/descent at 100 m · s−1 was more severe than that of 75 m · s−1 and 50 m · s−1 (P < 0.01), and that there were no significant differences in EB caused by rapid ascent/descent at 75 m · s−1
and 50 m · s−1 (P > 0.05). There were no significant differences in pathological injuries at the altitudes of 6562 ft and 13,123 ft (P > 0.05). 3) Based on tympanic membrane structures, tympanometry, and histopathological results, rabbits’
EB can be classified into mild, moderate, and severe.DISCUSSION: EB’s dynamic models could be established through HCTs on rabbits with Eustachian tube dysfunction.Wang B, Xu X, Lin J, Jin Z. Dynamic rabbit model of ear barotrauma. Aerosp Med Hum Perform. 2019;
90(8):696–702.
Recurrent Middle Ear Barotrauma in Student Pilots
Authors: Sohn Jung Heob
Published: July 2019

INTRODUCTION: The present study evaluated the efficacy of the nine-step inflation-deflation tympanometric test for predicting recurrence of middle ear barotrauma.METHODS: Student pilots who were diagnosed with middle ear barotrauma from October 2010 to April 2011 were
enrolled. The grade of barotrauma was assigned using Teed’s classification. All subjects underwent tympanometry and nine-step inflation-deflation testing at first visit and after improvement. Recurrence was monitored for 2 wk after they resumed flight. The relationships among the recurrence
of middle ear barotrauma, the grade and duration of disease, and tympanometric and nine-step test results were evaluated.RESULTS: There were 35 cases enrolled. According to Teed’s classification, 16 cases were Grade 0 (45.7%) and 11 cases were Grade I (31.4%). Grade III was
shown in seven cases (20.0%) and one subject was Grade IV (2.9%). The mean duration of illness was 5.9 d. In the initial 9-step tests, 29 subjects (85.3%) failed to pass the entire test. On follow-up tests, eight cases (22.9%) failed. Seven pilots (20.0%) showed recurrent middle ear barotrauma
after resumed flight. All of the cases with recurrence failed the follow-up nine-step tests. The nine-step test showed high sensitivity and specificity values for predicting recurrence. A clear correlation was observed between recurrence and nine-step test results.DISCUSSION: The
nine-step inflation-deflation test provides useful information about Eustachian tube function. The nine-step test can be useful to reduce the risk of recurrence of middle ear barotrauma in pilots.Sohn JH. Recurrent middle ear barotrauma in student pilots. Aerosp Med Hum Perform.
2019; 90(8):681–687.
Spacecraft Maximum Allowable Concentrations for Manganese Compounds in Mars Dust
Authors: Romoser Amelia A. , Ryder Valerie E. , McCoy J. Torin
Published: July 2019

INTRODUCTION: Exposure to excess manganese (Mn) can cause multiple toxicological outcomes in humans, most notably neurotoxicity. Ample epidemiological evidence suggests that chronic, low-level exposure causes subclinical cognitive effects. Because NASA astronauts will be exposed
to Mars regolith, Spacecraft Maximum Allowable Concentrations (SMACs) were developed following an extensive literature review.METHODS: Multiple databases were searched for information relevant to derivation of Mn SMAC values. An additional search for Mars dust data was performed.
Risk assessment approaches were applied, including adjustments for space-relevant susceptibility to Mn effects, to develop limits for 1-h to 1000-d exposures. Rover data informed the assessment and enabled calculation of allowable total dust exposure based on Mn content.RESULTS:
Over 400 relevant sources were identified. Applicability of exposure characteristics and data collection methods influenced key study choice. SMACs ranging from 3 mg · m−3 (1 h) – 0.0079 mg · m−3 (1000 d) were set to protect primarily against
neurocognitive and respiratory effects. Considering 0.38 wt% total Mn presence in the dust, maximum recommended total dust exposure should not exceed 790 mg · m−3 (1 h) – 2 mg · m−3 (1000 d).DISCUSSION: This literature review
allowed for identification of relevant studies to inform SMAC development. Manganese is one of several components to consider when developing an appropriate total dust limit for Martian dust; other dust elements may alter Mn bioavailability. Mission-specific activities may require alteration
of assumptions regarding Mn dust concentration and exposure duration. However, based on expected toxicity of particulate matter itself, the acute SMACs are protective, even with transient exposure during activities that could produce higher concentrations.Romoser AA, Ryder VE, McCoy
JT. Spacecraft maximum allowable concentrations for manganese compounds in Mars dust. Aerosp Med Hum Perform. 2019; 90(8):709–719.
Adaptation of Systemic and Pulmonary Circulation to Acute Changes in Gravity and Body Position
Authors: Hoffmann Uwe , Koschate Jessica , Appell Coriolano Hans-Joachim , Drescher Uwe , Thieschäfer Lutz , Dumitrescu Daniel , Werner Andreas
Published: July 2019

INTRODUCTION: Changes in gravity or body position provoke changes in hydrostatic pressure in the arterial system and in venous return. Potential asymmetries between left (QLV) and right ventricular (QRV) cardiac output during transient gravity changes were
investigated. It was hypothesized that blood volume is temporarily stored in the pulmonary vessels, with amount and duration depending on the level and directions of gravity.METHODS: Eight healthy, male subjects (32 ± 3 yr, 182 ± 7 cm, 82 ± 6 kg) were tested
on a tilt seat (TS), in a long arm human centrifuge (laHC), and during parabolic flights (PF). The gravitational changes during PF were reconstructed by changing gravity in a laHC and different body positions on a TS. All participants were tested in the seated, resting position. Heart rate
and blood pressure were recorded continuously and QLV was calculated, applying the Modelflow Algorithm. Gas exchange was measured breath-by-breath. QRV was calculated from these data according to the Fick Principle. Four sequences were superimposed and analyzed by ANOVA
with the factors Time, Ventricle (QRV, QLV), and Mode (TS, PF, laHC).RESULTS: After reductions in gravity QRV and QLV were transiently desynchronized. ANOVA showed no main effect for Mode, but significant changes were found for Time and
Ventricle and all interactions.DISCUSSION: Phases of reduced gravity seem to lead to transiently increased storage of blood volume inside the pulmonary vascular system. A more detailed understanding of these mechanisms might help to describe the compliance of the pulmonary vascular
system in diseases of the pulmonary circulation.Hoffmann U, Koschate J, Appell Coriolano H-J, Drescher U, Thieschäfer L, Dumitrescu D, Werner A. Adaptation of systemic and pulmonary circulation to acute changes in gravity and body position. Aerosp Med Hum Perform. 2019;
90(8):688–695.
Cardiovascular Risk Assessment in Pilots
Authors: Mulloy Andrew , Wielgosz Andreas
Published: July 2019

BACKGROUND: For over 30 yr the global medical community has attempted to define the acceptable cardiovascular risk in pilots. This challenge is compounded by the ever-changing technological and medical landscape of air travel. We aimed to review the existing literature on estimating
the risk of pilot cardiovascular incapacitation and determine if the current guidelines are founded in the best available evidence.METHODS: A detailed review of the guidelines and literature that supports them was completed. Relevant articles were identified by review of the source
literature of the guidelines and the references of these source documents. All articles referenced were reviewed in full by both authors. Data that informed the existing recommendations were reviewed and compared to available modern data. The results of these findings were incorporated into
a formula that allows for the calculation of acceptable pilot cardiovascular risk given any operator-determined set of variables.RESULTS: Among the evidence that informs current guidelines, there exists a need for further updating. A number of assumptions have been made in creating
guidelines and these may no longer reflect the current technological or medical aviation environment. Incorporating the identified variables into a formula allows for the calculation of acceptable cardiovascular risk. This formula was tested using past data and reproduced existing results.DISCUSSION:
Current guidelines for pilot cardiovascular risk assessment require review by the international aviation medical community. We propose a novel formula that may serve as a template for future guidelines and may be adapted as aviation technology and health data evolve.Mulloy A, Wielgosz
A. Cardiovascular risk assessment in pilots. Aerosp Med Hum Perform. 2019; 90(8):730–734.
Peculiarities of Changes of Young Swimmers’ Anthropometric Profile in the Time of Intensive Training and Its Correlation with Sports Results
Authors: Zuozienė Ilona Judita , Drevinskaitė Asta
Published: July 2019

Background. Knowledge of the peculiarities of anthropometric indicators, body weight components, and somatotype of athletes is important for coaches, sports scientists, and sports physicians at all stages of perennial training, starting with children’s sport. Often this is seen as an important indicator for identifying talented children in sport. The aim of this study was to determine how the anthropometric and body weight profile of young swimmers (11–12 years) significantly changes during the training period, and the correlation of these indicators with the competition results in the chosen event.Methods. The study included 24 young (aged 11.7 ± 0.5 years) national level swimmers – girls (n = 14) and boys (n = 10) with 3 to 4 years of sports experience in the national competitions and demonstrating high results in their age group. We investigated longitudinal anthropometric measurements, body mass components, established the somatotype and handgrip strength. The change of indicators was analysed during the training period of five months as well as its correlation with the result in the chosen sport. Sports results were rated by FINA ranking points.Results. The anthropometric longitudinal and transverse dimensions of girls and boys increased during the study period, however, there was no difference between the sex groups except for the length of the foot (it was higher in the group of boys, p < .05). The analysis of body weight components showed that girls had higher body fat mass and a percentage of body fat than boys (p < .05). The results of boys’ swimming had statistically significant links with their height (r = .857), foot length (r = .805), body weight (r = .857), lean mass and muscle mass (r = .927) and right handgrip strength (.786). There was no significant correlation between these indicators in the group of girls.Conclusion. The results of the study suggest that the characteristics of the anthropometric profile as a prognostic indicator for the viability in the chosen sport for boys were more significant than for girls.
PENGARUH LISAT PLATELET FASE SIKLUS MENSTRUASI TERHADAP AKTIVITAS FIBROBLAS KULIT MANUSIA
Authors: - Thianti Sylviningrum , - Flandiana Yogianti , - Sunardi Radiono , Wirohadidjojo Yohanes Widodo
Published: July 2019

Lisat platelet dari platelet rich fibrin (PRF) adalah material berisi growth factors, estradiol (E2), dan leptin. Pada fase ovulasi (FO) dijumpai kadar E2, jumlah platelet, dan leukosit tertinggi, dimana interaksinya dapat menghasilkan growth factors yang meningkatkan aktivitas fibroblas dermis. Penelitian ini bertujuan untuk mengetahui efek lisat platelet dari PRF fase bleeding (FB) dan FO pada aktivitas fibroblas dermis.Biakan fibroblas dermis dibedakan menjadi 3 kelompok, biakan dalam media kultur dan fetal bovine serum (FBS) 1% sebagai kontrol negatif, biakan dalam media kultur dan FBS 10% sebagai kontrol positif. Kelompok perlakuan adalah biakan fibroblas yang diberi lisat platelet dari PRF FB dan FO 10%, 20%, dan 40% selama 72 jam.Perbedaan bermakna (p<0,05) dijumpai pada rerata indeks proliferasi antara kelompok kontrol positif dengan fibroblas terpajan lisat platelet dari PRF FB dan FO 20% dibandingkan dengan kontrol negatif, serta rerata densitas optik (DO) timbunan kolagen dari fibroblas yang diberi lisat platelet dari PRF FB dan FO 20% dibandingkan dengan semua kelompok kontrol. Tidak ada perbedaan bermakna (p>0,05) pada rerata indeks proliferasi dan DO timbunan kolagen antara kelompok fibroblas yang terpajan semua konsentrasi lisat platelet dari PRF FB dan FO. Peningkatan aktivitas fibroblas dermis oleh lisat platelet dari PRF FO tidak berbeda dibandingkan dengan lisat platelet dari PRF FB.Kata kunci : lisat platelet, platelet rich fibrin, fase bleeding, fase ovulasi, fibroblas, kolagen
DERMATOFITOSIS GENERALISATA PADA PASIEN LIMFOMA NON HODGKIN
Authors: Wardani Meidina Kusuma , Kartayana Johan
Published: July 2019

Pada beberapa keadaan, infeksi dermatofita dapat menyebabkan suatu keadaan infeksi yang luas, khususnya pada pasien imunokompromais, misalnya pada kasus keganasan dan HIV.Seorang perempuan berusia 50 tahun dengan diagnosis Limfoma Non Hodgkin dikonsulkan dari poliklinik Penyakit Dalam dengan lesi berupa plak eritematosa, multipel, berbentuk anular, polisiklik dengan tepi aktif (central healing) pada regio kapitis, fasialis, colli, torakalis, abdominalis, braki dekstra, antebrachii dextra et sinistra, manus dekstra, inguinal, gluteal, dan ekstremitas inferior yang disertai dengan rasa gatal. Pada pasien kemudian dilakukan pemeriksaan KOH 10% didapatkan gambaran struktur jamur berupa hifa dan pemeriksaan biakan ditemukan gambaran Trichophyton rubrum. Pasien diberikan terapi berupa itrakonazol oral sebanyak 2 x 100 mg yang diberikan selama 2 minggu.Pada kasus ini infeksi dermatofita luas terjadi akibat keadaan imunokompromais yang disebabkan oleh Limfoma Non Hodgkin yang diderita oleh pasien maupun akibat kemoterapi. Terapi pilihan pada kasus ini adalah itrakonazol oral yang memberikan hasil sangat baik setelah 2 minggu.Kata kunci: dermatofitosis, imunokompromais, Limfoma Non Hodgkin