bluuebunny/crossref_metadata_2025 huggingface dataset:
Seismic Response of Reinforced-Concrete One-Storey Precast Industrial Buildings with Horizontal Cladding Panels
Authors: Gams Matija , Starešinič Gabrijela , Isaković Tatjana
Published: October 2023

An extensive parametric study of the seismic response of one-storey precast buildings with horizontal cladding panels frequently used in Central Europe was conducted to analyse the panels’ influence on the overall response of buildings and to find out if the panels can be considered non-structural elements when they are attached to the main building with the connections typically used in practice in Central Europe. The studied structural system consisted of reinforced concrete columns and beams connected by dowels. Horizontal cladding panels were attached to columns using one of the most frequently used isostatic fastening systems. The top connections provided out-of-plane stability, and the bottom connections supported the panel in the vertical direction. The parametric study was preceded by extensive experimental research, including cyclic tests on connections and full-scale shaking table tests of whole buildings. The results of experiments were used to reveal the basic response mechanisms of panels and connections and to develop, validate and calibrate numerical models employed in the parametric study presented herein. Fifteen generalised structures with different masses and heights were subjected to 30 accelerograms with two peak ground acceleration (PGA) intensities of 0.3 g and 0.5 g, corresponding to significant damage and near-collapse limit states. The effects of the construction imperfections in connections, the silicon sealant panel-to-panel connections and different types of connections of the bottom panel to the foundation were analysed. The crucial parameter influencing the response was the displacement capacity of the connections, which was considerably affected by the construction imperfections and, consequently, difficult to estimate. It has been observed that in some buildings, particularly in shorter structures with smaller mass, cladding panels can have a somewhat more notable influence on the overall response. However, in general, when the considered types of connections are used, the panels can be considered as non-structural elements, which do not importantly influence the response of the main building. Owing to structural imperfections and relatively short available gaps, the failure of the considered top connections and falling of the panels is very likely in the high seismicity regions. In the most adverse cases, it can occur even in the moderate seismicity regions.
Pyrolyzed POMs@ZIF-67 Exhibiting High Performance as Direct Glucose Fuel Cell Anode Catalysts
Authors: Jiao Shipu , Kang Ning , Zhang Yihao , Li Yang , Maryam Bushra , Zhang Xu , Zhang Pingping , Liu Xianhua
Published: October 2023

Polyoxometalates (POMs) are three-dimensional materials with unique, exceptional physical and chemical characteristics. The performance of POM-derived materials is anticipated to be enhanced by the combination of POM and metal–organic frameworks (MOFs) due to the high surface areas of MOF materials. In this study, three kinds of T-POMs@ZIF-67 (T-PMo@ZIF-67, T-SiW@ZIF-67, and T-PW@ZIF-67) were prepared by doping a cobalt-based MOF (ZIF-67) with three POMs (phosphomolybdic acid, silicotungstic acid, and phosphotungstic acid). The results show that the power density of the T-PMo@ZIF-67 catalyst anode is 3.08 times that of the blank control anode and 1.34 times that of the CoMoO4 catalyst. These findings suggest that the synthesis of MOF derivatives by doping MOFs with POM will have significant potential for use in the fuel cell industry.
Axon-Autonomous Effects of the Amyloid Precursor Protein Intracellular Domain (AICD) on Kinase Signaling and Fast Axonal Transport
Authors: König Svenja , Schmidt Nadine , Bechberger Karin , Morris Sarah , Priego Mercedes , Zaky Hannah , Song Yuyu , Pielage Jan , Brunholz Silke , Brady Scott T. , Kins Stefan , Morfini Gerardo
Published: October 2023

The amyloid precursor protein (APP) is a key molecular component of Alzheimer’s disease (AD) pathogenesis. Proteolytic APP processing generates various cleavage products, including extracellular amyloid beta (Aβ) and the cytoplasmic APP intracellular domain (AICD). Although the role of AICD in the activation of kinase signaling pathways is well established in the context of full-length APP, little is known about intracellular effects of the AICD fragment, particularly within discrete neuronal compartments. Deficits in fast axonal transport (FAT) and axonopathy documented in AD-affected neurons prompted us to evaluate potential axon-autonomous effects of the AICD fragment for the first time. Vesicle motility assays using the isolated squid axoplasm preparation revealed inhibition of FAT by AICD. Biochemical experiments linked this effect to aberrant activation of selected axonal kinases and heightened phosphorylation of the anterograde motor protein conventional kinesin, consistent with precedents showing phosphorylation-dependent regulation of motors proteins powering FAT. Pharmacological inhibitors of these kinases alleviated the AICD inhibitory effect on FAT. Deletion experiments indicated this effect requires a sequence encompassing the NPTY motif in AICD and interacting axonal proteins containing a phosphotyrosine-binding domain. Collectively, these results provide a proof of principle for axon-specific effects of AICD, further suggesting a potential mechanistic framework linking alterations in APP processing, FAT deficits, and axonal pathology in AD.
Editorial for the Special Issue “Current Development of Pediatric Minimally Invasive Surgery” of the Journal Children
Authors: Bergholz Robert , Krebs Thomas Franz
Published: October 2023

Dear colleagues, [...]
A Higher Amount of Nutritional Intake as a Possible Cause of Hyperglycemia in Extremely Premature Infants in Parenteral and Enteral Nutrition at the Tertiary Neonatal Intensive Care Unit
Authors: Predanič Drobne Iza , Kornhauser Cerar Lilijana , Erčulj Vanja , Grosek Štefan
Published: October 2023

Background: This study aimed to find an association between infants who had hyperglycemia and those who did not, those treated with insulin or not and several prenatal and postnatal variables or the suboptimal prescription of parenteral nutrition. Methods: We conducted a retrospective study, which included extremely premature infants (<28 weeks of gestation) admitted to the tertiary NICU, University Medical Centre Ljubljana, between 1 January 2021 and 31 December 2021. Blood glucose measurements, insulin treatment, general characteristics, nutritional data and complications of prematurity were obtained retrospectively from hospital data. RESULTS: There were 21 infants included in the study who did not receive insulin and 17 who were treated with insulin. Infants receiving insulin were younger and lighter compared to the non-insulin treatment group (mean gestational age 178 vs. 188 days; median birth weight 680 g vs. 990 g). The younger insulin group of infants received the same daily number of total macronutrients per kg per day compared to the older non-insulin group, i.e., glucose, lipids and amino acids, as recommended for the gestational age and birth weight. After adjusting for gestational age, no significant association with complications of prematurity was found. Conclusions: The postulated explanation (with the prescription of a higher amount of macronutrients during the first seven days) for hyperglycemia and treatment with insulin in the less mature and lighter infants cannot be supported by the data given.
Mathematical Investigation of the Infection Dynamics of COVID-19 Using the Fractional Differential Quadrature Method
Authors: Mohamed M. , Mabrouk S. M. , Rashed A. S.
Published: October 2023

In recent times, the global community has been faced with the unprecedented challenge of the coronavirus disease (COVID-19) pandemic, which has had a profound and enduring impact on both global health and the global economy. The utilization of mathematical modeling has become an essential instrument in the characterization and understanding of the dynamics associated with infectious illnesses. In this study, the utilization of the differential quadrature method (DQM) was employed in order to anticipate the characterization of the dynamics of COVID-19 through a fractional mathematical model. Uniform and non-uniform polynomial differential quadrature methods (PDQMs) and a discrete singular convolution method (DSCDQM) were employed in the examination of the dynamics of COVID-19 in vulnerable, exposed, deceased, asymptomatic, and recovered persons. An analysis was conducted to compare the methodologies used in this study, as well as the modified Euler method, in order to highlight the superior efficiency of the DQM approach in terms of code-execution times. The results demonstrated that the fractional order significantly influenced the outcomes. As the fractional order tended towards unity, the anticipated numbers of vulnerable, exposed, deceased, asymptomatic, and recovered individuals increased. During the initial week of the inquiry, there was a substantial rise in the number of individuals who contracted COVID-19, which was primarily attributed to the disease’s high transmission rate. As a result, there was an increase in the number of individuals who recovered, in tandem with the rise in the number of infected individuals. These results highlight the importance of the fractional order in influencing the dynamics of COVID-19. The utilization of the DQM approach, characterized by its proficient code-execution durations, provided significant insights into the dynamics of COVID-19 among diverse population cohorts and enhanced our comprehension of the evolution of the pandemic. The proposed method was efficient in dealing with ordinary differential equations (ODEs), partial differential equations (PDEs), and fractional differential equations (FDEs), in either linear or nonlinear forms. In addition, the stability of the DQM and its validity were verified during the present study. Moreover, the error analysis showed that DQM has better error percentages in many applications than other relevant techniques.
The Meaning of Successful School Leadership in Disadvantaged Contexts in Spain: Approach from the International Successful School Principalship Project (ISSPP)
Authors: Moral-Santaella Cristina , Raso-Sánchez Francisco
Published: October 2023

Successful school leadership in disadvantaged contexts is a topic of debate in the context of the Spanish education system. There needs to be clear identification of the concept of school success and what successful school leadership means. Thanks to the research carried out within the ISSPP project in Spain, we have been able to expand our knowledge on how success is defined in disadvantaged contexts: How have principals contributed to their school’s success? How has success grown and been sustained? And how have successful principals overcome internal and external factors to build a strong professional identity that enables them to fight for social justice? The ISSPP research in Spain finally concludes that the leadership variable may be considered decisive in the learning outcomes of pupils. It is not only the context variable that would be decisive in academic results, but rather leadership capable of making the moral purpose of education a reality.
TEM Strata Inversion Imaging with IP Effect Based on Enhanced GCN by Extracting Long-Dependency Features
Authors: Li Ruiheng , Di Yi , Tian Hao , Gan Lu
Published: October 2023

Utilizing neural network models to inverse time-domain electromagnetic signals enables rapid acquisition of electrical structures, a non-intrusive method widely applied in geological and environmental surveys. However, traditional multi-layer perceptron (MLP) feature extraction is limited, struggling with cases involving complex electrical media with induced polarization effects, thereby limiting the inversion model’s predictive capacity. A graph-topology-based neural network model for strata electrical structure imaging with long-dependency feature extraction was proposed. We employ graph convolutional networks (GCN) for capturing non-Euclidean features like resistivity-thickness coupling and Long Short-Term Memory (LSTM) to capture long-dependency features. The LSTM compensates for GCN’s constraints in capturing distant node relationships. Using case studies with 5-strata and 9-strata resistivity models containing induced polarization effects, compared to traditional MLP networks, the proposed model utilizing time-domain features and graph-topology-based electrical structure extraction significantly improves performance. The mean absolute error in inversion misfit is reduced from 10–20% to around 2–3%.
Primary Energy Consumption Patterns in Selected European Countries from 1990 to 2021: A Cluster Analysis Approach
Authors: Kosowski Piotr , Kosowska Katarzyna , Janiga Damian
Published: October 2023

This study delves into the structure of primary energy consumption in European countries, utilizing data from the Eurostat database, and focuses on the years 1990 and 2021. Through cluster analysis, countries were categorized based on their consumption patterns, revealing significant insights into energy security. The findings indicate a discernible shift away from solid fossil fuels, with renewable energy sources witnessing the most substantial growth. Natural gas, serving as a transitional fuel, has seen a rise in consumption, while nuclear energy’s development remained relatively stagnant. Oil, despite its declining share, remains a crucial component in the European energy mix. The study also highlights the challenges and implications of over-reliance on a single energy source, emphasizing the need for a diversified energy strategy. The analysis underscores the importance of diversifying primary energy sources to ensure energy security. While renewable sources are environmentally favorable, their inherent instability necessitates backup from other energy sources. Solid fossil fuels, despite their availability, face challenges due to environmental concerns. Natural gas, while flexible, requires extensive infrastructure and is highly politicized. Nuclear energy, despite its potential as an ideal complement to renewables, faces barriers in terms of investment and public perception. Oil, though convenient, is a fossil source with associated CO2 emissions and largely needs to be imported. In conclusion, the study advocates for a well-diversified set of energy sources tailored to individual country-specific situations, emphasizing the importance of strategic planning in energy consumption to ensure long-term energy security.
Study on Gasoline–Air Mixture Explosion Overpressure Characteristics and Flame Propagation Behaviors in an Annular Cylindrical Confined Space with a Circular Arch
Authors: Jiang Xinsheng , Chen Ri , Zhang Peili , Cai Yunxiong , Zhou Dongliang , He Donghai , Qin Xizhuo , Zhu Shijie
Published: October 2023

Gasoline–air mixture explosions mostly occur in buried tank rooms, which are annular cylindrical confined spaces with circular arches. In this paper, explosion experiments at different gasoline–air mixture volume fractions are carried out in an annular cylindrical steel bench with a circular arch curvature radius of 900 mm and an annular half-perimeter to radial width ratio of 12π. The results show that the development process of explosion overpressure is clearly divided into four stages after first-order differentiation treatment. Compared with other types of confined spaces, 1.70% is still the most dangerous gasoline–air mixture volume fraction. However, this type of confined space has a larger inner surface area in the same volume condition, which will inevitably increase the heat absorption rate, reduce the chemical reaction rate, and slow down the flame propagation speed. Meanwhile, this spatial structure will inevitably make the explosion flames collide, which will promote positive feedback coupling between explosion flames and pressure waves, making the explosion more violent and dangerous. These results can provide theoretical and technical support for the explosion prevention design of buried tank rooms.
Soil Organic Carbon Estimation in Ferrara (Northern Italy) Combining In Situ Geochemical Analyses and Hyperspectral Remote Sensing
Authors: Salani Gian Marco , Lissoni Michele , Bianchini Gianluca , Brombin Valentina , Natali Stefano , Natali Claudio
Published: October 2023

This study investigated whether surface soil organic carbon (SOC) content could be estimated using hyperspectral data provided by the Italian Space Agency PRISMA satellite. We collected 100 representative topsoil samples in an area of 30 × 30 Km2 in the province of Ferrara (Northern Italy), estimated their SOC content and other soil properties through thermo-gravimetric analysis, and matched these to the spectra of the sampled areas that were measured by PRISMA on 7 April 2020. A tentative model was created for SOC estimation using ordinary least-squares (OLS) regression and an artificial neural network (ANN). Repeated k-fold cross-validation of the OLS and ANN models yielded R2 values of 0.64 and 0.49, respectively. The performance of the models was inferior to that obtained from the literature using similar modeling techniques in relatively small areas (up to 3 × 3 Km2) and characterized by restricted SOC variability (0.2–2.1 wt%). However, our data were collected over a wider area with high SOC content variability (0.7–9.3 wt%); consequently, significant variations were observed over a spatial scale of just a few meters. Therefore, this work shows the importance of testing remote sensing techniques for SOC measurements in more complex areas than those reported in the existing literature. Furthermore, our study sheds light on the geolocation errors and missing data of PRISMA.
The Influence of Listening to Preferred versus Non-Preferred Music on Static and Dynamic Balance in Middle-Aged Women
Authors: Waer Fatma Ben , Alexe Cristina Ioana , Tohănean Dragoș Ioan , Čaušević Denis , Alexe Dan Iulian , Sahli Sonia
Published: October 2023

Although many women perform postural tasks while listening to music, no study has investigated whether preferred music has different effects than non-preferred music. Thus, this study aimed to explore the effects of listening to preferred versus non-preferred music on postural balance among middle-aged women. Twenty-four women aged between 50 and 55 years were recruited for this study. To assess their static balance, a stabilometric platform was used, recording the mean center of pressure velocity (CoPVm), whereas the timed up and go test (TUGT) was used to assess their dynamic balance. The results showed that listening to their preferred music significantly decreased their CoPVm values (in the firm-surface/eyes-open (EO) condition: (p < 0.05; 95% CI [−0.01, 2.17])). In contrast, when the women were listening to non-preferred music, their CoPVm values significantly (p < 0.05) increased compared to the no-music condition in all the postural conditions except for the firm-surface/EO condition. In conclusion, listening to music has unique effects on postural performance, and these effects depend on the genre of music. Listening to preferred music improved both static and dynamic balance in middle-aged women, whereas listening to non-preferred music negatively affected these performances, even in challenged postural conditions.
Efferocytosis and Respiratory Disease
Authors: Zheng Wenxue , Zhou Zhengjie , Guo Xiaoping , Zuo Xu , Zhang Jiaqi , An Yiming , Zheng Haoyu , Yue Yuan , Wang Guoqiang , Wang Fang
Published: October 2023

Cells are the smallest units that make up living organisms, which constantly undergo the processes of proliferation, differentiation, senescence and death. Dead cells need to be removed in time to maintain the homeostasis of the organism and keep it healthy. This process is called efferocytosis. If the process fails, this may cause different types of diseases. More and more evidence suggests that a faulty efferocytosis process is closely related to the pathological processes of respiratory diseases. In this review, we will first introduce the process and the related mechanisms of efferocytosis of the macrophage. Secondly, we will propose some methods that can regulate the function of efferocytosis at different stages of the process. Next, we will discuss the role of efferocytosis in different lung diseases and the related treatment approaches. Finally, we will summarize the drugs that have been applied in clinical practice that can act upon efferocytosis, in order to provide new ideas for the treatment of lung diseases.
Interaction of Synthetic Cannabinoid Receptor Agonists with Cannabinoid Receptor I: Insights into Activation Molecular Mechanism
Authors: Gavryushov Sergei , Bashilov Anton , Cherashev-Tumanov Konstantin V. , Kuzmich Nikolay N. , Burykina Tatyana I. , Izotov Boris N.
Published: October 2023

Synthetic cannabinoid receptor agonists (SCRAs) have become a wide group of new psychoactive substances since the 2010s. For the last few years, the X-ray structures of the complexes of cannabinoid receptor I (CB1) with SCRAs as well as the complexes of CB1 with its antagonist have been published. Based on those data, SCRA–CB1 interactions are analyzed in detail, using molecular modeling and molecular dynamics simulations. The molecular mechanism of the conformational transformation of the transmembrane domain of CB1 caused by its interaction with SCRA is studied. These conformational changes allosterically modulate the CB1–Gi complex, providing activation of the Gi protein. Based on the X-ray-determined structures of the CB1–ligand complexes, a stable apo conformation of inactive CB1 with a relatively low potential barrier of receptor activation was modeled. For that model, molecular dynamic simulations of SCRA binding to CB1 led to the active state of CB1, which allowed us to explore the key features of this activation and the molecular mechanism of the receptor’s structural transformation. The simulated CB1 activation is in accordance with the previously published experimental data for the activation at protein mutations or structural changes of ligands. The key feature of the suggested activation mechanism is the determination of the stiff core of the CB1 transmembrane domain and the statement that the entire conformational transformation of the receptor to the active state is caused by a shift of alpha helix TM7 relative to this core. The shift itself is caused by protein–ligand interactions. It was verified via steered molecular dynamics simulations of the X-ray-determined structures of the inactive receptor, which resulted in the active conformation of CB1 irrespective of the placement of agonist ligand in the receptor’s active site.
Intervention of AXL in EGFR Signaling via Phosphorylation and Stabilization of MIG6 in Non-Small Cell Lung Cancer
Authors: Yang Ya-Yu , Lin Sheng-Chieh , Lay Jong-Ding , Cho Chun-Yu , Jang Te-Hsuan , Ku Hsiu-Ying , Yao Chih-Jung , Chuang Shuang-En
Published: October 2023

About 80% of lung cancer patients are diagnosed with non–small cell lung cancer (NSCLC). EGFR mutation and overexpression are common in NSCLC, thus making EGFR signaling a key target for therapy. While EGFR kinase inhibitors (EGFR–TKIs) are widely used and efficacious in treatment, increases in resistance and tumor recurrence with alternative survival pathway activation, such as that of AXL and MET, occur frequently. AXL is one of the EMT (epithelial–mesenchymal transition) signature genes, and EMT morphological changes are also responsible for EGFR–TKI resistance. MIG6 is a negative regulator of ERBB signaling and has been reported to be positively correlated with EGFR–TKI resistance, and downregulation of MIG6 by miR–200 enhances EMT transition. While MIG6 and AXL are both correlated with EMT and EGFR signaling pathways, how AXL, MIG6 and EGFR interplay in lung cancer remains elusive. Correlations between AXL and MIG6 expression were analyzed using Oncomine or the CCLE. A luciferase reporter assay was used for determining MIG6 promoter activity. Ectopic overexpression, RNA interference, Western blot analysis, qRT–PCR, a proximity ligation assay and a coimmunoprecipitation assay were performed to analyze the effects of certain gene expressions on protein–protein interaction and to explore the underlying mechanisms. An in vitro kinase assay and LC–MS/MS were utilized to determine the phosphorylation sites of AXL. In this study, we demonstrate that MIG6 is a novel substrate of AXL and is stabilized upon phosphorylation at Y310 and Y394/395 by AXL. This study reveals a connection between MIG6 and AXL in lung cancer. AXL phosphorylates and stabilizes MIG6 protein, and in this way EGFR signaling may be modulated. This study may provide new insights into the EGFR regulatory network and may help to advance cancer treatment.
Factors Influencing Marker Expressions of Cultured Human Cord Blood-Derived Mast Cells
Authors: Alimohammadi Shahrzad , Masuda-Kuroki Kana , Szöllősi Attila Gábor , Di Nardo Anna
Published: October 2023

Mast cells (MCs) are tissue-resident immune cells of a hematopoietic origin that play vital roles in innate and adaptive immunity. Human MCs can be isolated and differentiated from various tissue sources, including cord blood, when supplemented with cytokines such as stem cell factor, interleukin 3, and interleukin 6. Our current research study has shown significant differences in the marker expressions of human cord blood-derived mast cells (hCBMCs) based on donor dependency and the type of medium used for culturing and differentiation. These findings are particularly relevant given the challenges of obtaining specialty media influencing MC phenotypic marker expressions. We found that hCBMCs cultured in StemSpanTM-XF medium had a moderate expression of mast/stem cell growth factor receptor Kit (c-KIT) (mRNA and protein), low expressions of FcεRI (mRNA) and TLR2 (mRNA and protein) but had high levels of MRGPRX2 (mRNA and protein) expressions. In contrast, hCBMCs cultured in Stem Line II medium expressed FcεRI and TLR2 (mRNA and protein) with higher c-KIT but had lower MRGPRX2 expressions compared to the hCBMCs cultured in the StemSpanTM-XF medium. These results suggest that it is crucial to consider both donor dependency and the medium when investigating MC functions and that further research is needed to fully understand the impact of these factors on the hCBMC marker expressions.
β-Lactam TRPM8 Antagonists Derived from Phe-Phenylalaninol Conjugates: Structure–Activity Relationships and Antiallodynic Activity
Authors: Martín-Escura Cristina , Bonache M. Ángeles , Medina Jessy A. , Medina-Peris Alicia , De Andrés-López Jorge , González-Rodríguez Sara , Kerselaers Sara , Fernández-Ballester Gregorio , Voets Thomas , Ferrer-Montiel Antonio , Fernández-Carvajal Asia , González-Muñiz Rosario
Published: October 2023

The protein transient receptor potential melastatin type 8 (TRPM8), a non-selective, calcium (Ca2+)-permeable ion channel is implicated in several pathological conditions, including neuropathic pain states. In our previous research endeavors, we have identified β-lactam derivatives with high hydrophobic character that exhibit potent and selective TRPM8 antagonist activity. This work describes the synthesis of novel derivatives featuring C-terminal amides and diversely substituted N′-terminal monobenzyl groups in an attempt to increase the total polar surface area (TPSA) in this family of compounds. The primary goal was to assess the influence of these substituents on the inhibition of menthol-induced cellular Ca2+ entry, thereby establishing critical structure–activity relationships. While the substitution of the tert-butyl ester by isobutyl amide moieties improved the antagonist activity, none of the N′-monobencyl derivatives, regardless of the substituent on the phenyl ring, achieved the activity of the model dibenzyl compound. The antagonist potency of the most effective compounds was subsequently verified using Patch-Clamp electrophysiology experiments. Furthermore, we evaluated the selectivity of one of these compounds against other members of the transient receptor potential (TRP) ion channel family and some receptors connected to peripheral pain pathways. This compound demonstrated specificity for TRPM8 channels. To better comprehend the potential mode of interaction, we conducted docking experiments to uncover plausible binding sites on the functionally active tetrameric protein. While the four main populated poses are located by the pore zone, a similar location to that described for the N-(3-aminopropyl)-2-[(3-methylphenyl)methoxy]-N-(2-thienylmethyl)-benzamide (AMTB) antagonist cannot be discarded. Finally, in vivo experiments, involving a couple of selected compounds, revealed significant antinociceptive activity within a mice model of cold allodynia induced by oxaliplatin (OXA).
Modeling the Homologous Recombination Process: Methods, Successes and Challenges
Authors: Sabei Afra , Prentiss Mara , Prévost Chantal
Published: October 2023

Homologous recombination (HR) is a fundamental process common to all species. HR aims to faithfully repair DNA double strand breaks. HR involves the formation of nucleoprotein filaments on DNA single strands (ssDNA) resected from the break. The nucleoprotein filaments search for homologous regions in the genome and promote strand exchange with the ssDNA homologous region in an unbroken copy of the genome. HR has been the object of intensive studies for decades. Because multi-scale dynamics is a fundamental aspect of this process, studying HR is highly challenging, both experimentally and using computational approaches. Nevertheless, knowledge has built up over the years and has recently progressed at an accelerated pace, borne by increasingly focused investigations using new techniques such as single molecule approaches. Linking this knowledge to the atomic structure of the nucleoprotein filament systems and the succession of unstable, transient intermediate steps that takes place during the HR process remains a challenge; modeling retains a very strong role in bridging the gap between structures that are stable enough to be observed and in exploring transition paths between these structures. However, working on ever-changing long filament systems submitted to kinetic processes is full of pitfalls. This review presents the modeling tools that are used in such studies, their possibilities and limitations, and reviews the advances in the knowledge of the HR process that have been obtained through modeling. Notably, we will emphasize how cooperative behavior in the HR nucleoprotein filament enables modeling to produce reliable information.
New Supersonic Nozzle Test Rig Used to Generate Condensing Flow Test Data According to Barschdorff
Authors: Maqueo Martínez Manuel Ernesto , Schippling Stefan , Schatz Markus , Vogt Damian M.
Published: October 2023

Considerable progress has been achieved in recent decades in understanding the phenomena related to the onset of condensation in steam flows, both experimentally and especially numerically. Nevertheless, there is still a certain disagreement between the different numerical models used. Unfortunately, the available experimental validation data are not sufficiently detailed to allow for proper validation of computational fluid dynamics (CFD) simulations. Therefore, this paper presents new experimental data for condensing steam flows, acquired in a supersonic nozzle according to Barschdorff, at the Institute of Thermal Turbomachinery Laboratory (ITSM) at the University of Stuttgart. A steady inlet pressure of approximately 784 mbar was set for three inlet temperatures down to 100.2 ∘C. Condensation onset is accurately captured across the nozzle, using down to 1 mm spatial resolution for both pneumatic and light spectra measurements. CFD simulations were performed using the commercial solver ANSYS CFX. The droplet diameters are numerically overestimated by approximately a factor of 1.5. Disagreement has been found between original Barschdorff’s experiments and measurements at ITSM. However, there is a good agreement in terms of the pressure distribution along the nozzle axis between experimental and numerical results. The reproducibility of the results is excellent.
Development of a Public-Domain Measure of Two-Dimensional Rotation Ability and Preliminary Evidence for Discriminant Validity among Occupations
Authors: Mather Kendall A. , Condon David M.
Published: October 2023

Despite their known influence in science, technology, engineering, and mathematics (STEM) fields, spatial abilities remain an underassessed aspect of cognition, particularly in educational settings. One explanation could be a lack of affordable, valid instruments for measuring various aspects of spatial ability. We evaluate the validity of a set of public-domain, algorithmically generated two-dimensional rotation items using a sample from the Synthetic Aperture Personality Assessment (SAPA) Project (N = 1,020,195). We examine the psychometric properties of the items and their relationship with various other cognitive abilities and personality traits. In addition, we identify the highest performing college majors and occupations on the 2D rotation items and on a set of 3D rotation items. Findings suggest strong unidimensionality for the 2D rotation items and the presence of lower-order factors which reflect differences across items in mental rotation demands. The highest scoring majors and occupations were similar—but not identical—across the 2D and 3D rotation measures and point to potentially meaningful differences across areas of expertise.
CFD Study of the Non-Linear Physical Phenomena of the TLP of a 15-MW-Class FOWT under Extreme Waves
Authors: Ha Yoon-Jin , Kim Kyong-Hwan , Park Ji-Yong
Published: October 2023

In this study, a numerical simulation was conducted to investigate the non-linear physical phenomena of a tension leg platform (TLP) of a 15-MW-class floating offshore wind turbine (FOWT). Computational fluid dynamics was employed as the numerical tool, and a deforming mesh technique was used to describe the moving body. To examine the non-linear physical phenomena, an irregular wave was generated with a focus on head sea conditions. The springing and ringing responses were calculated from the numerical simulation results, and the relations between the motions and dynamic tensions of the 15-MW-class FOWT TLP were investigated. From the irregular wave impact simulation, it was found that the springing response via the wave sum frequencies and the ringing response occurred at approximately three times the wave peak frequency. Additionally, whipping simulations were conducted under a focused wave. The results show that the response in pitch resonance frequency was caused by the wave impact. The numerical results of this study could be used as fundamental data for FOWT TLP design.
Differential Recognition of Clinically Relevant Sporothrix Species by Human Granulocytes
Authors: Galván-Hernández Ana K. , Gómez-Gaviria Manuela , Martínez-Duncker Iván , Martínez-Álvarez José A. , Mora-Montes Héctor M.
Published: October 2023

Sporotrichosis is a cutaneous mycosis that affects humans and animals and has a worldwide distribution. This infection is mainly caused by Sporothrix schenckii, Sporothrix brasiliensis, and Sporothrix globosa. Current research about anti-Sporothrix immunity has been mainly focused on S. schenckii and S. brasiliensis, using different types of human or animal immune cells. Granulocytes are a group of cells relevant for cytokine production, with the capacity for phagocytosis and the generation of neutrophil extracellular traps (NETs). Considering their importance, this study aimed to compare the capacity of human granulocytes to stimulate cytokines, uptake, and form NETs when interacting with different Sporothrix species. We found that conidia, germlings, and yeast-like cells from S. schenckii, S. brasiliensis, and S. globosa play an important role in the interaction with these immune cells, establishing morphology- and species-specific cytokine profiles. S. brasil-iensis tended to stimulate an anti-inflammatory cytokine profile, whilst the other two species had a proinflammatory one. S. globosa cells were the most phagocytosed cells, which occurred through a dectin-1-dependent mechanism, while the uptake of S. brasiliensis mainly occurred via TLR4 and CR3. Cell wall N-linked and O-linked glycans, along with β-1,3-glucan, played a significant role in the interaction of these Sporothrix species with human granulocytes. Finally, this study indicates that conidia and yeast-like cells are capable of inducing NETs, with the latter being a better stimulant. To the best of our knowledge, this is the first study that reports the cytokine profiles produced by human granulocytes interacting with Sporothrix cells.
Hyperbolic-Secant-Function-Based Fast Sliding Mode Control for Pantograph Robots
Authors: Hassan Muhammad Ali , Cao Zhenwei , Man Zhihong
Published: October 2023

Technological advancements in control systems are indispensable and require a robust controller with fast convergence in numerous sectors like robotics, space engineering, etc. A sliding mode controller (SMC) is a robust controller when subjected to nonlinearities, external disturbances, and uncertainties in system dynamics. However, SMC is prone to chattering, which can damage the plant in long term and results in low tracking accuracy. In this research paper, a novel hyperbolic secant function (HSF)-based SMC is proposed that achieves fast convergence, reduces chattering, and improves tracking performance. Stability analysis is conducted based on Lyapunov stability criteria. Comparative simulations and experiments on a pantograph robot (PR) show the effectiveness of the proposed algorithm.
Monoclonal Gammopathy of Renal Significance with Deposits of Infrequent Morphology: Two Case Reports of Light and Heavy Chain Deposition Disease with Atypical Presentation and Literature Review
Authors: De La Flor José C. , Monroy-Condori Maribel , Apaza-Chavez Jacqueline , Arenas-Moncaleano Iván , Díaz Francisco , Guerra-Torres Xavier E. , Morales-Montoya Jorge L. , Lerma-Verdejo Ana , Sandoval Edna , Villa Daniel , Vieru Coca-Mihaela
Published: October 2023

Background: Monoclonal immunoglobulin deposition disease (MIDD) includes three entities: light chain deposition disease (LCDD), heavy chain deposition disease (HCDD) and light and heavy chain deposition disease (LHCDD). The renal presentation can manifest with varying degrees of proteinuria and/or nephrotic syndrome, microhematuria, and often leads to end-stage renal disease. Given the rarity of LHCDD, therapeutic approaches for this condition remain inconclusive, as clinical trials are limited. Case presentation: We report two male patients with underlying monoclonal gammopathy of renal significance (MGRS) associated with LHCDD lesions. Both cases had non-nephrotic proteinuria, moderately impaired renal function, and normal levels of C3 and C4. Light microscopy of the renal biopsies in both patients did not show lesions of nodular glomerulosclerosis. Immunofluorescence showed a staining pattern with interrupted linear IgA-κ in patient #1 and IgA-λ in patient #2 only along the glomerular basement membrane (GBM). Electron microscopy of patient #1 revealed electrodense deposits in the subendothelial and mesangial areas only along the GBM. Discussion: In this case series, we discuss the clinical, analytical, and histopathological findings of two rare cases of LHCDD. Both patients exhibited IgA monoclonality and were diagnosed with monoclonal gammopathy of undetermined significance (MGUS) by the hematology department at the time of renal biopsy. Treatment with steroids and cytotoxic agents targeting the clone cells responsible for the deposition disease resulted in a favorable renal and hematologic response.
New Monoterpene Glycoside Paeoniflorin Derivatives as NO and IL-1β Inhibitors: Synthesis and Biological Evaluation
Authors: Chen Yongjie , Zhang Guoqing , Cao Dongyi , Wang Fei , Zhang Fan , Shao Huawu , Jiao Wei
Published: October 2023

Several monoterpene glycoside compounds were extracted from Paeonia lactiflora Pall. Among them, paeoniflorin, a water-soluble monoterpene glycoside found in the root of Paeonia lactiflora Pall, exhibits excellent antioxidant pharmacological functions. Initially, Sc(CF3SO3)3 was employed as the catalyst for paeoniflorin’s dehydration and rearrangement reactions with alcohols. Subsequently, structural modifications were performed on paeoniflorin through a series of responses, including acetylation, deacetylation, and debenzoylation, ultimately yielding 46 monoterpene glycoside derivatives. The potential inhibitory effects on the pro-inflammatory mediators interleukin-1 beta (IL-1β) and nitric oxide (NO) were assessed in vitro. The results revealed that compounds 29 and 31 demonstrated notable inhibition of NO production, while eight derivatives (3, 8, 18, 20, 21, 29, 34, and 40) displayed substantial inhibitory effects on the secretion of IL-1β. Computational research was also undertaken to investigate the binding affinity of the ligands with the target proteins. Interactions between the proteins and substrates were elucidated, and corresponding binding energies were calculated accordingly. The findings of this study could provide valuable insights into the design and development of novel anti-inflammatory agents with enhanced pharmacological properties.
The Formulation and Evaluation of Deep Eutectic Vehicles for the Topical Delivery of Azelaic Acid for Acne Treatment
Authors: Luhaibi Dhari K. , Ali Hiba H. Mohammed , Al-Ani Israa , Shalan Naeem , Al-Akayleh Faisal , Al-Remawi Mayyas , Nasereddin Jehad , Qinna Nidal A. , Al-Adham Isi , Khanfar Mai
Published: October 2023

The current work was aimed at the development of a topical drug delivery system for azelaic acid (AzA) for acne treatment. The systems tested for this purpose were deep eutectic systems (DESs) prepared from choline chloride (CC), malonic acid (MA), and PEG 400. Three CC to MA and eight different MA: CC: PEG400 ratios were tested. The physical appearance of the tested formulations ranged from solid and liquid to semisolid. Only those that showed liquid formulations of suitable viscosity were considered for further investigations. A eutectic mixture made from MA: CC: PEG400 1:1:6 (MCP 116) showed the best characteristics in terms of viscosity, contact angle, spreadability, partition coefficient, and in vitro diffusion. Moreover, the MCP116 showed close rheological properties to the commercially available market lead acne treatment product (Skinorin®). In addition, the formula showed synergistic antibacterial activity between the MA moiety of the DES and the AzA. In vitro diffusion studies using polyamide membranes demonstrated superior diffusion of MCP116 over the pure drug and the commercial product. No signs of skin irritation and edema were observed when MCP116 was applied to rabbit skin. Additionally, the MCP116 was found to be, physically and chemically, highly stable at 4, 25, and 40 °C for a one-month stability study.
DFT Study of WS2-Based Nanotubes Electronic Properties under Torsion Deformations
Authors: Domnin Anton V. , Mikhailov Ilia E. , Evarestov Robert A.
Published: October 2023

In this study, the influence of torsional deformations on the properties of chiral WS2-based nanotubes was investigated. All calculations presented in this study were performed using the density functional theory (DFT) and atomic gaussian type orbitals basis set. Nanotubes with chirality indices (8, 2), (12, 3), (24, 6) and (36, 9) corresponding to diameters of 10.68 Å, 14.90 Å, 28.26 Å and 41.90 Å, respectively, are examined. Our results reveal that for nanotubes with smaller diameters, the structure obtained through rolling from a slab is not optimal and undergoes spontaneous deformation. Furthermore, this study demonstrates that the nanotube torsion deformation leads to a reduction in the band gap. This observation suggests the potential for utilizing such torsional deformations to enhance the photocatalytic activity of the nanotubes.
Re-Emergence of HMPV in Gwangju, South Korea, after the COVID-19 Pandemic
Authors: Cho Sun-Ju , Kim Sun-Hee , Lee Hongsu , Lee Yeong-Un , Mun Jeongeun , Park Sujung , Park Jungwook , Park Ji-Su , Lee Kwangho , Lee Cheong-mi , Seo Jinjong , Kim Yonghwan , Chung Yoon-Seok
Published: October 2023

The non-pharmaceutical interventions implemented to prevent the spread of COVID-19 have affected the epidemiology of other respiratory viruses. In South Korea, Human metapneumovirus (HMPV) typically occurs from winter to the following spring; however, it was not detected for two years during the COVID-19 pandemic and re-emerged in the fall of 2022, which is a non-epidemic season. To examine the molecular genetic characteristics of HMPV before and after the COVID-19 pandemic, we analyzed 427 HMPV-positive samples collected in the Gwangju area from 2018 to 2022. Among these, 24 samples were subjected to whole-genome sequencing. Compared to the period before the COVID-19 pandemic, the incidence rate of HMPV in 2022 increased by 2.5-fold. Especially in the age group of 6–10 years, the incidence rate increased by more than 4.5-fold. In the phylogenetic analysis results, before the COVID-19 pandemic, the A2.2.2 lineage was predominant, while in 2022, the A2.2.1 and B2 lineage were observed. The non-pharmaceutical interventions implemented after COVID-19, such as social distancing, have reduced opportunities for exposure to HMPV, subsequently leading to decreased acquisition of immunity. As a result, HMPV occurred during non-epidemic seasons, influencing the age distribution of its occurrences.
Tailoring Wavelength-Selective Diffraction Efficiency Using Triple-Layer Double-Relief Blazed Gratings Incorporating Materials with Intersecting Dispersion Curves
Authors: Schmidt Lia , Hillmer Hartmut , Brunner Robert
Published: October 2023

Diffractive optical elements (DOEs) fundamentally provide the possibility to simultaneously utilize multiple orders for different imaging functions within a system. However, to take advantage of this property, it is necessary to tailor the assignment of specific wavelengths or wavelength ranges with high diffraction efficiency to specific diffraction orders. To achieve this wavelength-selective assignment to different orders, simple diffractive profile shapes are not suitable; instead, multilayer DOEs are required. In this study, we conducted theoretical, scalar investigations on the diffraction efficiency of triple-layer double-relief DOEs for the purpose of tailored wavelength selectivity. Specific materials such as nanocomposites, layer materials, and high-refractive-index liquids with strong dispersion were included, in addition to inorganic glasses, to enable wide design freedom for wavelength selectivity across multiple orders. To simultaneously account for both positive and negative orders, specific material combinations featuring intersecting or touching dispersion curves were utilized. For various material combinations, we calculated significantly different efficiency profiles for multiple orders by varying the relief depths. Further, we discuss the possibility of fine-tuning the efficiency profiles by using high-index liquids as an intermediate layer between two solid profiles, whose dispersion properties can be varied continuously or at least in small steps.