bluuebunny/crossref_metadata_2025 huggingface dataset:
Is Covid-19 “vaccine uptake” in postsecondary education a “problem”? A critical policy inquiry
Authors: Chaufan Claudia
Published: November 2023

Since the launch of the Covid-19 global vaccination campaign, postsecondary institutions have strongly promoted vaccination, often through mandates, and the academic literature has identified “vaccine uptake” among postsecondary students as a problem deserving monitoring, research, and intervention. However, with the admission that vaccines do not stop viral spread, that older-age and co-morbidities are major determinants of poor outcomes, and that many vaccine side effects disproportionately affect the young, it cannot be assumed that a risk-benefit analysis favors vaccinating postsecondary students. Drawing from critical policy studies, I appraise the literature on Covid-19 vaccine uptake in postsecondary education. I find that this literature reflects the “scientific consensus,” hardly acknowledging contradictory medical evidence, ignoring coercive elements underlying “vaccine acceptance,” and neglecting ethical tensions built into the very design of vaccination policies. I discuss potential explanations for my findings, and their implications for academia’s role in society in the COVID-19 era and beyond.
“We’re not the first and we’re not going to be the last”: Perspectives of system-involved black and Latinx young adults on racial injustice during the 2020 black lives matter protests
Authors: Reed Taylor A , Abrams Laura S , Bondoc Christopher , Barnert Elizabeth S
Published: November 2023

This study explores how Black and Latinx young adults (ages 18–25) who were reentering the community from Los Angeles County jails viewed racial injustice in the criminal legal system in the context of the Black Lives Matter (BLM) protests of summer 2020. A sample of nine young adults participated in a series of up to nine monthly interviews between June 2020 and May 2021. The participants included seven young adults who identified as Black and two who identified as Latinx. Overall, participants held negative views of the criminal legal system and felt that police officers harmed Black and Brown people and communities. While most participants expressed support for the BLM protests, others doubted the protests as an effective tactic to address racial injustice. Even those who supported the protests described doubts about the possibility of genuine systemic changes in the criminal legal system and society. Findings pose implications for cultivating optimism for social change and countering legal cynicism among system-involved young adults.
Peer-based research funding as a model for journalism funding
Authors: Latos Maria , Lobigs Frank , Wormer Holger
Published: November 2023

Financing high-quality journalistic reporting is becoming increasingly difficult worldwide and economic pressure has intensified in the wake of the COVID-19 pandemic. While numerous alternative funding possibilities are discussed, ranging from membership models to government funding, they should not compromise the highest possible independence of journalism – a premise that also applies to scientific research. Here, the state is involved in funding, but peer review models reduce funding bias. However, systematic approaches as to how established funding models in research could be transferred to journalism are lacking. We attempt such a systematic transfer using the example of the German Research Foundation ( Deutsche Forschungsgemeinschaft, DFG). The transfer, based on an analysis of the complex DFG funding structures, was validated in 10 interviews with experts from science, journalism and foundations. Building on this, we developed a concept for a German Journalism Foundation ( Deutsche Journalismus-gemeinschaft, DJG), which awards funding to journalists and cooperative projects based on a peer review process. The funding priorities of the proposed organization range from infrastructure support to grants for investigative skills. Thus, unlike other models, it does not focus on funding specific topics in media coverage, but on areas such as innovation support, technology implementation and training. Although the model was designed for Germany, such a systematic transfer could also be tested for other countries.
Clinical Significance of Soluble LAG-3 (sLAG-3) in Patients With Cervical Cancer Determined via Enzyme-Linked Immunosorbent Assay With Monoclonal Antibodies
Authors: Li Yang , Wang Wenwen , Tian Jingluan , Zhou Ying , Shen Yu , Wang Mingyuan , Tang Longhai , Liu Cuiping , Zhang Xueguang , Shen Fangrong , Chen Youguo , Gu Yanzheng
Published: November 2023

Background: The tumor microenvironment and tumor immunity have become the focus of research on tumor diagnosis and treatment. Lymphocyte activation gene-3 (LAG-3, CD223) is a newly discovered immunosuppressive receptor that is abnormally expressed in various tumor microenvironments and plays an important role as an immune checkpoint in the tumor immune response. Objective: We developed a novel enzyme-linked immunosorbent assay kit, examined the levels of soluble LAG-3 (sLAG-3) in the serum of patients with cervical cancer, and identified new biomarkers for cervical cancer development. Methods: To investigate the potential biological function of sLAG-3, we generated and characterized 2 novel anti-LAG-3 monoclonal antibodies, namely 4F4 and 4E12. We performed western blotting, immunofluorescence, and immunohistochemistry using hybridoma technology and an enzyme-linked immunosorbent assay kit for detecting human sLAG-3 based on an improved double-antibody sandwich enzyme-linked immunosorbent assay method. The stability and sensitivity of these kits were also assessed. Results: We screened and characterized 2 novel monoclonal antibodies against human LAG-3. The enzyme-linked immunosorbent assay kit also includes a wide range of tests. Using this enzyme-linked immunosorbent assay system, we found that the expression level of sLAG-3 in the peripheral blood of patients with cervical cancer significantly decreased as the disease progressed ( P < .0001). Multivariate logistic regression analysis revealed that low sLAG-3 expression was an independent predictor of cervical cancer and related diseases ( P < .05). Furthermore, receiver operating characteristic curve analysis showed that sLAG-3 had diagnostic value for cervical cancer metastasis ( P < .0001). Conclusion: These data suggest that sLAG-3 is a potential biomarker for cervical cancer development. Therefore, this kit has a certain application value in the diagnosis of cervical cancer.
The Neuropathological Impacts of COVID-19: Challenges and Alternative Treatment Options for Alzheimer’s Like Brain Changes on Severely SARS-CoV-2 Infected Patients
Authors: Rahman Md. Rashidur , Akter Raushanara , Neelotpol Sharmind , Mayesha Iffat Islam , Afrose Afrina
Published: November 2023

Recently, some researchers claimed neuropathological changes lead to Alzheimer’s-like brains after severe infection of SARS-CoV-2. Several mechanisms have been postulated on how SARS-CoV-2 neurological damage leads to Alzheimer's disease (AD) development. Neurobiochemical changes during infection may significantly induce Alzheimer's disease in severely COVID-19 infected people. The immune system is also compromised while infected by this novel coronavirus. However, recent studies are insufficient to conclude the relationship between Alzheimer's disease and COVID-19. This review demonstrates the possible pathways of neuropathological changes induced by the SARS-CoV-2 virus in AD patients or leading to AD in COVID-19 patients. Therefore, this study delineates the challenges for COVID-19 infected AD patients and the mechanism of actions of natural compounds and alternative treatments to overcome those. Furthermore, animal studies and a large cohort of COVID-19 survivors who showed neuroinflammation and neurological changes may augment the research to discover the relationship between Alzheimer's disease and COVID-19.
Complexing Properties of Synthesized 1,3,5-Triaza-7-Phosphaadamantane Derivatives Towards Some Lanthanides and Transition Metal Cations With Significant Antimicrobial and Antioxidant Activities
Authors: Baklouti Lassaad , Mellah Besma , Koko Waleed S.
Published: November 2023

The synthesis of new water-soluble N-alkylated derivatives of 1,3,5-triaza-7-phosphaadamantane is presented. Ru(PPh3)2Cl2 has been used to react with 1-(4-nitrobenzyl)-3,5-triaza-1-azonia-7-phosphaadamantane bromide (PTAR). By using elemental analysis, NMR, and IR spectroscopy, the obtained compounds were identified. The UV-visible absorption spectroscopy has been used to monitor the complexation of various transition metal cations. Studies on conductivity have been utilized to validate the complexes’ stoichiometries. Using the disc diffusion method, five bacteria strains were used for the study of the antimicrobial activity of compounds 1-3. All tested pathogens, including M luteus LB 141107, were found to have strong biologic activity against the compounds tested in this study. Additionally, DPPH (2,2-diphenyl-1-picrylhydrazyl) has been tested for its ability to scavenge hydrogen peroxide and free radicals. According to our results, these compounds exhibit excellent radical scavenging properties.
Community and hospital academic performance of working nurse interns: A mixed-methods study in Peru
Authors: Moya-Salazar Jeel , Quispe-Pariona Roxana , Cañari Betsy , Moya-Salazar Belén , Goicochea-Palomino Eliane A
Published: November 2023

Introduction and Objective:

Nursing is a professional career that requires patient-specialized care. To this end, it requires a high academic performance during undergraduate studies. However, some factors that can influence the academic performance of nursing students have been noticed during the internship. We aimed to determine the hospital and community-academic performance of nurse interns who work.

Materials and Methods:

We designed a 3-year mixed study carried out with students of the Universidad Norbert Wiener. We interviewed 15 students about their academic performance, the consequences of studying while working, internship, family, and academic activities. Using the quantitative approach, we analyzed the evaluations of 321 students between 2016 and 2018.

Results:

The qualitative approach showed that students had certain difficulties in their clinical internship because they worked and studied simultaneously. Despite this, their academic performance was remarkable in both internship programs, and 10% of students had an outstanding performance. This performance is subject to personal, economic, and family factors that affect students in their last year of undergraduate studies.

Conclusions:

Working nursing interns had a remarkable academic performance during community and hospital internships. This performance is influenced by personal, financial, and family factors that affect students in their final year of undergraduate study.
Terms-we-serve-with: Five dimensions for anticipating and repairing algorithmic harm
Authors: Rakova Bogdana , Shelby Renee , Ma Megan
Published: November 2023

Power and information asymmetries between people and digital technology companies are further legitimized through contractual agreements that fail to provide meaningful consent and contestability. In particular, the Terms-of-Service (ToS) agreement, is a contract of adhesion where companies effectively set the terms and conditions of the contract. Whereas, ToS reinforce existing structural inequalities, we seek to enable an intersectional accountability mechanism grounded in the practice of algorithmic reparation. Building on existing critiques of ToS in the context of algorithmic systems, we return to the roots of contract theory by recentering notions of agency and mutual assent. We evolve a multipronged intervention we frame as the Terms-we-Serve-with (TwSw) social, computational, and legal framework. The TwSw is a new social imaginary centered on: (1) co-constitution of user agreements, through participatory mechanisms; (2) addressing friction, leveraging the fields of design justice and critical design in the production and resolution of conflict; (3) enabling refusal mechanisms, reflecting the need for a sufficient level of human oversight and agency including opting out; (4) complaint and algorithmic harms reporting, through a feminist studies lens and open-sourced computational tools; and (5) disclosure-centered mediation, to disclose, acknowledge, and take responsibility for harm, drawing on the field of medical law. We further inform our analysis through an exploratory design workshop with a South African gender-based violence reporting AI startup. We derive practical strategies for communities, technologists, and policy-makers to leverage a relational approach to algorithmic reparation and propose there's a need for a radical restructuring of the “take-it-or-leave-it” ToS agreement.
Clinical algorithms, racism, and “fairness” in healthcare: A case of bounded justice
Authors: El-Azab Sarah , Nong Paige
Published: November 2023

To date, attempts to address racially discriminatory clinical algorithms have largely focused on fairness and the development of models that “do no harm.” While the push for fairness is rooted in a desire to avoid or ameliorate health disparities, it generally neglects the role of racism in shaping health outcomes and does little to repair harm to patients. These limitations necessitate reconceptualizing how clinical algorithms should be designed and employed in pursuit of racial justice and health equity. A useful lens for this work is bounded justice, a concept and research analytic proposed by Melissa Creary to guide multidisciplinary health equity interventions. We describe how bounded justice offers a lens for (1) articulating the deep injustices embedded in the datasets, methodologies, and sociotechnical infrastructure underlying design and implementation of clinical algorithms and (2) envisioning how these algorithms can be redesigned to contribute to larger efforts that not only address current inequities, but to redress the historical mistreatment of communities of color by biomedical institutions. Thus, the aim of this article is two-fold. First, we apply the bounded justice analytic to fairness and clinical algorithms by describing structural constraints on health equity efforts such as medical device regulatory frameworks, race-based medicine, and racism in data. We then reimagine how clinical algorithms could function as a reparative technology to support justice and empower patients in the healthcare system.
Assessment and Analysis of the Complexities in Sustainability of the Transport Projects Under CPEC: A Grounded Theory Approach
Authors: Ahmed Waqas , Ali Sharafat , Asghar Muhammad , Ismailov Alisher
Published: November 2023

This study proposes a modern research framework for establishing a grounded theory perspective on regional development initiatives along the China-Pakistan Economic Corridor (CPEC). Grounded theory is a well-known research approach that uses comparative analysis to generate hypotheses from analyzed data. Thematic event analysis exposes the transitions between domains throughout CPEC project design and execution, including political, economic, social, technical, legal, and environmental elements. The Orange Line Metro Train (OLMT) project’s axial coding highlights contextual variables such as problematic agreements, circumvention of regulations, lack of preparation, public indignation, heritage demolition, tree cutting, and regional tensions. These elements, coupled with China-Pakistan relations, the CPEC framework, infrastructural conditions, and other considerations, impact top-down planning, bureaucratic difficulties, and mismanagement of government plans. Consequently, these techniques cause project delays, unsustainable subsidies, historical damage, and environmental implications, raising questions about accountability and political regime change. This chapter focuses on data collection by using the Grounded Theory Method. These ramifications go beyond the CPEC project, emphasizing the significance of consulting key parties and selecting projects based on scientific and technological criteria. Institutional strengthening, climate change policies, and a focus on sustainable development are all critical. Policy consequences include open and accountable resource allocation, enhanced planning and risk assessment, cost-cutting techniques, cultural heritage preservation, specialized courts, and investment in education and healthcare. It is also critical to strengthen democratic institutions and reduce the environmental dangers.
Perceptions on Birth Preparedness and Complication Readiness: Perspectives of Pregnant Women
Authors: Nachinab Gilbert Ti-enkawol , Yakong Vida Nyagre , Dubik Joshua Dindiok , Klutse Kokui Dziedzom , Asumah Mubarick Nungbaso , Bimpong Beatrice Nyarko , Mensah Cecilia , Sarpong Cynthia Anane
Published: November 2023

Birth preparedness and complication readiness (BPCR) involves preparing for childbirth, bearing in mind the possible complications. The concept of BPCR has the potential to improve the awareness and utilization of suitable medical facilities to ensure safe delivery and minimize maternal and neonatal mortality. The purpose of the study was to explore pregnant women’s perspectives on the benefits and barriers of BPCR in the Northern Ghana. The study employed an exploratory descriptive design using a qualitative approach. Purposive sampling was used to recruit 13 participants. Data was collected through individual face-to-face interviews. The data was analyzed using qualitative content analysis. Out of the 13 participants, the age range for participants was 17 to 37 years; more than half were married, but less than half had no formal education. The participants indicated their understanding of BPCR by explaining the concept of BPCR, dangers signs, and prevention of danger signs. A varied source of information on BPCR was reported including the midwives or clinic, media, and family. The participants indicated that the benefits of BPCR include ensuring adequate preparation, delivery of a healthy child, and arranging for support. The barriers to BPCR were inadequate information, lack of finances, lack of transport, lack of support from the family, and lack of community support. The study findings indicate that the participants were generally informed about the concept of BPCR. However, to enable pregnant women prepare adequately for childbirth, there is a need to involve the husbands in the education on BPCR. The use of mass media to get families and communities educated on the importance of BPCR will enable them to support pregnant women.
A Way of Human Capital Accumulation: Heterogeneous Impact of Shadow Education on Students’ Academic Performance in China
Authors: Tan Minda , Liu Shuiyun
Published: November 2023

Shadow education helps students become academically competitive. Currently, little is known about whether shadow education can effectively fulfill either remedial or enrichment purposes in China. Using the nationally representative data from the China Education Panel Survey (CEPS) collected in 2013 and 2014, this paper explored the opportunity gap in shadow education and its impact on Chinese eighth-grade students’ mathematics and English reading performance. This study further examined how this effect differs by students’ prior performance and school ranks by hierarchical linear modeling (HLM). The results show that affluent socioeconomic resources and parental pressure predicted larger chances of students using shadow education. On average, supplementary tutoring had a positive effect on academic achievement. After re-estimating the effect by groups, this study found that students disadvantaged by prior knowledge and quality of schooling benefited more from supplementary tutoring. The findings suggest that the expansion of shadow education may contribute to social reproduction within the current education system.
Pedagogic Exploration Into Adapting Automated Writing Evaluation and Peer Review Integrated Feedback Into Large-Sized University Writing Classes
Authors: Li Wei-Yan , Kau Kevin , Shiung Yi-Jiun
Published: November 2023

Automated Writing Evaluation (AWE) software has been viewed as a promising tool for assisting writing. This study integrated AWE and its combination with peer review discussion into writing practice in a large college writing class in the Asian context. Adopting a mixed-method approach, this study employed a quantitative questionnaire to investigate how students perceived the integration at different writing stages and a qualitative interview to examine what prompted their quantitative decisions. The results show that the integration of AWE and peer review feedback significantly reinforced the perceived usefulness of AWE at the revision stage, and students with different proficiency levels had notably different attitudes toward AWE. Their writing anxieties intensified as they became increasingly aware of their own weaknesses during the writing process. Therefore, student writing challenges involve not only language proficiency and writing skills but also psychological variables.
Factor Structure Validation and Measurement Invariance Testing of the Five C’s Model of Positive Youth Development among Emerging Adults in Malaysia
Authors: Zaremohzzabieh Zeinab , Krauss Steven , Nouri Katayoun Mehdinezhad
Published: November 2023

While validation of positive youth development (PYD) measures has increased considerably of late, validation of the Five C’s of PYD among emerging adults in non-WEIRD countries remains limited. The main objective of this study was to examine the factor structure, measurement invariance, and criterion-related validity of the Five C’s model of PYD through the use of the Positive Youth Development Inventory (PYDI). Data were collected from 436 university students in Malaysia, with a mean age of 21.19 ( SD = 1.19). The findings supported a modified bifactor model of the PYDI, which demonstrated strong measurement invariance across gender and race, but not across age categories. Connection showed the strongest latent factor correlations with the other four C’s. Criterion-related validity was supported through its association with psychological hardiness. The findings demonstrate adequate psychometric properties and cultural applicability of a modified bifactor model of the Five C’s model of PYD among emerging adults in Malaysia.
Metabolic and Sensory Components of Age-Related Hearing Loss: Associations With Distortion- and Reflection-Based Otoacoustic Emissions
Authors: Vaden Kenneth I. , Neely Stephen T. , Harris Sara E. , Dubno Judy R.
Published: November 2023

Age-related hearing loss is difficult to study in humans because multiple genetic and environmental risk factors may contribute to pathology and cochlear function declines in older adults. These pathologies, including degeneration of the stria vascularis, are hypothesized to affect outer hair cells responsible for active cochlear amplification of low-level sounds. Otoacoustic emission (OAE) measures are used to quantify the energy added to the traveling wave in cochlear amplification, which typically weakens with increased pure-tone thresholds and for older individuals. Thus, the current study evaluated two OAE measures for individuals with different components of age-related hearing loss. We examined two retrospective adult lifespan datasets (18 to 89+ years of age) from independent sites (Medical University of South Carolina and Boys Town National Research Hospital), which included demographics, noise history questionnaires, distortion-product otoacoustic emissions (DPOAE), and cochlear reflectance (CR). Metabolic and sensory estimates of age-related hearing loss were derived from the audiograms in each dataset, and then tested for associations with DPOAE and CR. The results showed that metabolic estimates increased for older participants and were associated with lower overall DPOAE and CR magnitudes across frequency (i.e., lower fitted intercepts). Sensory estimates were significantly higher for males, who reported more positive noise histories compared to females and were associated with steeper negative across-frequency slopes for DPOAEs. Although significant associations were observed between OAE configurations, DPOAEs appeared uniquely sensitive to metabolic estimates. The current findings suggest that distortion-based measures may provide greater sensitivity than reflection-based measures to the components of age-related hearing loss.
Anterior Cruciate Ligament Reconstruction With Iliotibial Band Autograft in Skeletally Immature Prepubescent Children: A Physeal-Sparing Technique
Authors: Schumacher Felipe C. , Galvão Pedro H. S. A. F. , Mameri Enzo S. , Credidio Marcos V. , Franciozi Carlos E. , Kubota Marcelo S.
Published: November 2023

Background:

As there has been an increasing incidence of anterior cruciate ligament (ACL) tears in children and adolescents in the past few decades, nonoperative management is seen to result in less favorable clinical outcomes, resulting in meniscal and cartilage damage, and arthritic changes. Despite the risk of iatrogenic physeal injuries with operative approach, several reconstruction options, such as physeal-sparing techniques for skeletally immature patients, are described to restore joint stability.

Indications:

We bring a combined extra-articular and intra-articular ACL reconstruction technique using autogenous iliotibial band (ITB), which is indicated for prepubescent patients (Tanner stages 1 or 2) with open physes, significant growth potential (≤11 years for girls and ≤12 years for boys) and smaller knees, where an all-epiphyseal ACL reconstruction would lack epiphyseal space

Technique Description:

The technique consists of a modified MacIntosh intra-articular and extra-articular ITB reconstruction, described by Micheli and further characterized by Kocher. The central portion of the ITB is harvested proximally (at least 15 cm) and left attached to Gerdy's tubercle distally. The harvested graft is brought from over-the-top posteriorly and passed under the intermeniscal ligament anteriorly through an epiphyseal groove on the tibia.

Results:

The outcomes shown for the Kocher ITB ACL reconstruction technique are favorable at a mean follow-up of 5.3 years: from 44 patients, 41 returned to sports involving cutting or pivoting, and the revision rate was low at 4.5%.

Discussion/Conclusion:

The awareness of physeal injury related to the operative treatment of ACL tears in the pediatric population has resulted in several physeal-sparing and physeal-respecting techniques of reconstruction. Some of these have shown to reduce the risk of physeal damage, maintaining stability and providing excellent functional outcomes, with low revision rates in long-term follow-up and minimal risk of growth disturbance.

Patient Consent Disclosure Statement:

The author(s) attests that consent has been obtained from any patient(s) appearing in this publication. If the individual may be identifiable, the author(s) has included a statement of release or other written form of approval from the patient(s) with this submission for publication.
Whole genome sequencing and pan-genome analysis of Staphylococcus/Mammaliicoccus spp. isolated from diabetic foot ulcers and contralateral healthy skin of Algerian patients
Authors: Ferhaoui Nerdjes , Tanaka Rina , Sekizuka Tsuyoshi , Kuroda Makoto , Sebaihia Mohammed
Published: November 2023

Background

Diabetic foot infections (DFIs) are the most common complications of diabetic foot ulcers (DFUs), and a significant cause of lower extremity amputation. In this study we used whole genome sequencing to characterize the clonal composition, virulence and resistance genetic determinants of 58 Staphylococcus/Mammaliicoccus spp. isolates from contralateral healthy skin and DFU from 44 hospitalized patients.

Results

S. aureus (n = 32) and S. epidermidis (n = 10) isolates were recovered from both DFUs and healthy skin, whereas, S. haemolyticus (n = 8), M. sciuri (n = 1), S. hominis (n = 1) and S. simulans (n = 3) were recovered exclusively from healthy skin. In contrast, S. caprae (n = 2) and S. saprophyticus (n = 1) were recovered only from DFUs. Among S. aureus isolates, MRSA were present with high prevalence (27/32, 84.4%), 18 of which (66.7%) were from DFUs and 9 (33.3%) from healthy skin. In contrast, the coagulase-negative Staphylococcus (CoNS)/Mammaliicoccus isolates (n = 26), in particular S. epidermidis and S. haemolyticus were more prevalent in healthy skin, (10/26, 38.5%) and (8/26, 30.8%), respectively. MLST, spa and SCCmec typing classified the 32 S. aureus isolates into 6 STs, ST672, ST80, ST241, ST1, ST97, ST291 and 4 unknown STs (STNF); 8 spa types, t044, t037, t3841, t1247, t127, t639, t937 and t9432 and 2 SCCmec types, type IV and type III(A). Among CoNS, the S. epidermidis isolates belonged to ST54, ST35 and ST640. S. haemolyticus belonged to ST3, ST25, ST29, ST1 and ST56. The sole M. sciuri isolate was found to carry an SCCmec type III(A). A wide range of virulence genes and antimicrobial resistance genes were found among our isolates, with varying distribution between species or STs. The pan-genome analysis revealed a highly clonal population of Staphylococcus isolates, particularly among S. aureus isolates. Interestingly, the majority of S. aureus isolates including MRSA, recovered from the healthy skin and DFUs of the same patient belonged to the same clone and exhibited similar virulence/resistance genotype.

Conclusions

Our study provides clinically relevant information on the population profile, virulence and antibiotic resistance of Staphylococcus/Mammaliicoccus spp. in DFIs, which could serve as a basis for further studies on these as well as other groups of pathogens associated with DFIs.
Transcriptomics and physiology reveal the mechanism of potassium indole-3-butyrate (IBAK) mediating rice resistance to salt stress
Authors: Zhou Hang , Liu Meiling , Meng Fengyan , Zheng Dianfeng , Feng Naijie
Published: November 2023

Background

IBAK, as a plant growth regulator, has broad application prospects in improving crop resistance to abiotic stress.

Results

In this study, the regulation mechanism of IBAK on rice was revealed by physiology and transcriptomics by spraying 80 mg·L−1 IBAK solution on rice leaves at the early jointing stage under salt stress. The results showed that spraying IBAK solution on leaves under salt stress could significantly increase K+ content, decrease Na+ content, increase net photosynthetic rate (Pn), and increase the activity of catalase (CAT) and the contents of glutathione (GSH) and soluble protein in rice leaves. Using IBAK under salt stress increased the expression of plant hormone signal transduction pathway-related genes LOC4332548 and LOC4330957, which may help rice to more effectively sense and respond to plant hormone signals and enhance resistance to salt stress. In addition, the photosynthesis pathway-related genes LOC4339270, LOC4327150, and LOC4346326 were upregulated after using IBAK under salt stress, and the upregulation of these genes may be beneficial to improve the efficiency of photosynthesis and increase the photosynthetic capacity of rice. Regarding starch and sucrose metabolism pathway, spraying IBAK on leaves could promote the expression of sucrose synthesis-related gene LOC4347800 and increase the expression of starch synthesis-related genes LOC4330709 and LOC4343010 under salt stress. Finally, IBAK spraying resulted in the upregulation of multiple 50 S and 30 S ribosomal protein genes in the ribosome pathway, which may increase protein synthesis, help maintain cell function, and promote rice growth and development.

Conclusion

The results of this study revealed the mechanism of IBAK mediating resistance to salt stress in rice.
Integrated transcriptome and metabolome analysis unveils the mechanism of color-transition in Edgeworthia chrysantha tepals
Authors: Zhou Ningzhi , Yan Yujuan , Wen Yafeng , Zhang Minhuan , Huang Yu
Published: November 2023

Background

Edgeworthia chrysantha, a deciduous shrub endemic to China, is known for its high ornamental value, extensive cultivation history, and wide-ranging applications. However, theoretical research on this plant is severely lacking. While its flowering process displays striking color transitions from green (S1) to yellow (S2) and then to white (S3), the scientific exploration of this phenomenon is limited, and the underlying regulatory mechanisms are yet to be elucidated.

Results

Correlation analysis between phenotypic measurements and pigment content revealed that carotenoids and chlorophyll are the key pigments responsible for the color changes. Metabolomic analysis of carotenoids demonstrated that lutein and β-carotene were present at higher levels in S1, while S2 exhibited increased diversity and quantity of carotenoids compared to other stages. Notably, antheraxanthin, zeaxanthin, lycopene, and α-cryptoxanthin showed significant increases. In S3, apart from the colorless phytoene, other carotenoid metabolites were significantly reduced to extremely low levels. Transcriptomic data indicated that PSY, Z-ISO, crtZ, ZEP, PDS and ZDS are key genes involved in carotenoid biosynthesis and accumulation, while NCED plays a crucial role in carotenoid degradation. SGR was identified as a key gene contributing to the progressive decline in chlorophyll content. Additionally, three transcription factors potentially regulating carotenoid metabolism were also identified.

Conclusions

This study represents the first systematic investigation, spanning from phenotypic to molecular levels, of the color-changing phenomenon in E. chrysantha. The study elucidates the crucial pigments, metabolites, genes, and transcription factors responsible for flower color changes during the flowering process, thereby providing preliminary understanding of the intrinsic regulatory mechanisms. These findings establish a theoretical foundation for the genetic improvement of flower color in E. chrysantha.
Inflammation and renal function decline in chronic coronary syndrome: a prospective multicenter cohort study
Authors: Kao Ting-Wei , Huang Chin-Chou , Leu Hsin-Bang , Yin Wei-Hsian , Tseng Wei-Kung , Wu Yen-Wen , Lin Tsung-Hsien , Yeh Hung-I , Chang Kuan-Cheng , Wang Ji-Hung , Wu Chau-Chung , Chen Jaw-Wen
Published: November 2023

Background

Renal function decline is a frequently encountered complication in patients with chronic coronary syndrome. Aside from traditional cardiovascular risk factors, the inflammatory burden emerged as the novel phenotype that compromised renal prognosis in such population.

Methods

A cohort with chronic coronary syndrome was enrolled to investigate the association between inflammatory status and renal dysfunction. Levels of inflammatory markers, including high-sensitivity C-reactive protein (hs-CRP), tumour necrosis factor-α (TNF-α), adiponectin, matrix metalloproteinase-9, interleukin-6, lipoprotein-associated phospholipase A2, were assessed. Renal event was defined as > 25% decline in estimated glomerular filtration rate (eGFR). Inflammatory scores were calculated based on the aggregate of hs-CRP, TNF-α, and adiponectin levels.

Results

Among the 850 enrolled subjects, 145 patients sustained a renal event during an averaged 3.5 years follow-up. Multivariate analysis with Cox regression suggested elevations in hs-CRP, TNF-α, and adiponectin levels were independent risk factors for the occurrence of a renal event. Whereas, Kaplan-Meier curve illustrated significant correlation between high TNF-α (P = 0.005), adiponectin (P < 0.001), but not hs-CRP (P = 0.092), and eGFR decline. The aggregative effect of these biomarkers was also distinctly correlated with renal events (score 2: P = 0.042; score 3: P < 0.001).

Conclusions

Inflammatory burden was associated with eGFR decline in patients with chronic coronary syndrome.
Circulating mir-199-3p screens the onset of type 2 diabetes mellitus and the complication of coronary heart disease and predicts the occurrence of major adverse cardiovascular events
Authors: Ruan Renjie , Liu Yanwei , Zhang Xiang
Published: November 2023

Background

Coronary heart disease (CHD) is a major complication of type 2 diabetes mellitus (T2DM), which causes an adverse prognosis. There is an urgent need to explore effective biomarkers to evaluate the patients’ adverse outcomes.

Objective

This study aimed to identify a novel indicator for screening T2DM and T2DM-CHD and predicting adverse prognosis.

Materials and methods

The study enrolled 52 healthy individuals, 85 T2DM patients, and 97 T2DM patients combined with CHD. Serum miR-199-3p levels in all study subjects were detected with PCR, and its diagnostic significance was evaluated by receiver operating curve (ROC) analysis. The involvement of miR-199-3p in disease development was assessed by the Chi-square test, and the logistic regression analysis was performed to estimate the risk factor for major adverse cardiovascular events (MACE) in T2DM-CHD patients.

Results

Significant downregulation of miR-199-3p was observed in the serum of both T2DM and T2DM-CHD patients, which discriminated patients from healthy individuals and distinguished T2DM and T2DM-CHD patients. Reduced serum miR-199-3p was associated with the increasing blood glucose, glycated hemoglobin (HbA1c), and homeostasis model assessment-insulin resistance index (HOMA-IR) of T2DM patients and the increasing triglycerides (TG), low-density lipoprotein (LDL), fibrinogen, and total cholesterol (TC) and decreasing high-density lipoprotein (HDL) of T2DM-CHD patients. miR-199-3p was also identified as a biomarker predicting the occurrence of MACE.

Conclusion

Downregulated miR-199-3p could screen the onset of T2DM and its complication with CHD. Reduced serum miR-199-3p was associated with the severe development of T2DM and T2DM-CHD and predicted the adverse outcomes of T2DM-CHD patients.
Feasibility and outcomes of using DIALOG+ in primary care to improve quality of life and mental distress of patients with long-term physical conditions: an exploratory non-controlled study in Bosnia and Herzegovina, Colombia and Uganda
Authors: van Loggerenberg Francois , Akena Dickens , Alinaitwe Racheal , Birabwa-Oketcho Harriet , Méndez Camilo Andrés Cabarique , Gómez-Restrepo Carlos , Kulenović Alma Džubur , Selak Nejra , Kiseljaković Meliha , Musisi Seggane , Nakasujja Noeline , Sewankambo Nelson K. , Priebe Stefan
Published: November 2023

Introduction

The management of long-term physical conditions is a challenge worldwide, absorbing a majority resources despite the importance of acute care. The management of these conditions is done largely in primary care and so interventions to improve primary care could have an enormous impact. However, very little data exist on how to do this. Mental distress is frequently comorbid with long term physical conditions, and can impact on health behaviour and adherence, leading to poorer outcomes. DIALOG+ is a low-cost, patient-centred and solution-focused intervention, which is used in routine patient-clinician meetings and has been shown to improve outcomes in mental health care. The question arises as to whether it could also be used in primary care to improve the quality of life and mental health of patients with long-term physical conditions. This is particularly important for low- and middle-income countries with limited health care resources.

Methods

An exploratory non-controlled multi-site trial was conducted in Bosnia and Herzegovina, Colombia, and Uganda. Feasibility was determined by recruitment, retention, and session completion. Patient outcomes (quality of life, anxiety and depression symptoms, objective social situation) were assessed at baseline and after three approximately monthly DIALOG+ sessions.

Results

A total of 117 patients were enrolled in the study, 25 in Bosnia and Herzegovina, 32 in Colombia, and 60 in Uganda. In each country, more than 75% of anticipated participants were recruited, with retention rates over 90% and completion of the intervention exceeding 92%. Patients had significantly higher quality of life and fewer anxiety and depression symptoms at post-intervention follow-up, with moderate to large effect sizes. There were no significant improvements in objective social situation.

Conclusion

The findings from this exploratory trial suggest that DIALOG+ is feasible in primary care settings for patients with long-term physical conditions and may substantially improve patient outcomes. Future research may test implementation and effectiveness of DIALOG+ in randomized controlled trials in wider primary care settings in low- and middle-income countries.

Trial registration

All studies were registered prospectively within the ISRCTN Registry. ISRCTN17003451, 02/12/2020 (Bosnia and Herzegovina), ISRCTN14018729, 01/12/2020 (Colombia) and ISRCTN50335796, 02/12/2020 (Uganda).
Public healthcare system utilization for chronic hepatitis C infection in Vietnam
Authors: Thi Ngoc Phuong Nguyen , My Ngoc Nghiem , Rasheed Sabrina , Khatun Fatema , Van Nuil Jennifer , Thanh Dung Nguyen , Mạnh Hung Le , Rahman Motiur
Published: November 2023

Background

Healthcare utilization is typically adversely affected when the treatment is expensive and requires multiple visits. We examined the determinants of healthcare-seeking for Hepatitis C virus (HCV) infection which is asymptomatic, chronic, and requires costly treatment in an urban tertiary care referral hospital in Vietnam.

Methods

We conducted a secondary analysis of hospital data for patients attending the Hospital for Tropical Diseases in Ho Chi Minh City, Vietnam between 2017 and 2020 specifically for HCV infection treatment. Poisson regression was used to determine the effect of personal factors (age, sex, comorbidities) and structural factors (health insurance, proximity to the facility, seasonality, year of visit) on the number of hospital visits.

Results

From 2017 to 2020 a total of 22,052 eligible patients sought treatment in the hospital. Among the patients, 50.4% were males and 58.7% were > 50 years of age. The mean number of visits per person was 2.17. In the multivariate analysis compared to 2017, the number of hospital visits increased by 4% in 2018 and then significantly decreased in 2019 and 2020. Visit numbers were significantly lower (6%) among South East region residents compared to those from Central Highlands and for those who lived further away from the hospital. The visit numbers were significantly higher among older age groups (5–11%), those with health insurance (6%), and those with comorbidities (5%) compared to others. Although the number of hospital visits by females was higher (7%) than males in 2017, it significantly decreased in subsequent years.

Conclusions

Our study indicated that there are both structural and individual factors affecting the number of visits for HCV treatment. To meet the global strategy for elimination of HCV, Vietnam Government needs to address the structural and personal barriers to healthcare seeking, with a special focus on women.
Impact of vaccination and variants of concern on long COVID clinical phenotypes
Authors: Kenny Grace , McCann Kathleen , O’Brien Conor , O’Broin Cathal , Tinago Willard , Yousif Obada , O’Gorman Tessa , Cotter Aoife G. , Lambert John S. , Feeney Eoin R. , de Barra Eoghan , Sadlier Corinna , Landay Alan , Doran Peter , Savinelli Stefano , Mallon Patrick W. G. ,   The All Ireland Infectious Diseases Cohort Study, MacCann Rachel , Garcia Leon Alejandro , Miles Sarah , Alalwan Dana , Negi Riya , Muldoon Eavan , Sheehan Gerard , McGinty Tara , Green Sandra , Leamy Kelly , Kelly Christine , de Barra Eoin , McConkey Samuel , Hurley Killain , Sulaiman Imran , Horgan Mary , Eustace Joseph , Bracken Tommy , Whelan Bryan , Low Justin , McNicholas Bairbre , Courtney Garry , Gavin Patrick
Published: November 2023

Background

Defining patterns of symptoms in long COVID is necessary to advance therapies for this heterogeneous condition. Here we aimed to describe clusters of symptoms in individuals with long COVID and explore the impact of the emergence of variants of concern (VOCs) and vaccination on these clusters.

Methods

In a prospective, multi centre cohort study, individuals with symptoms persisting > 4 weeks from acute COVID-19 were divided into two groups based on timing of acute infection; pre-Alpha VOC, denoted wild type (WT) group and post-Alpha VOC (incorporating alpha and delta dominant periods) denoted VOC group. We used multiple correspondence analysis (MCA) and hierarchical clustering in the WT and VOC groups to identify symptom clusters. We then used logistic regression to explore factors associated with individual symptoms.

Results

A total of 417 individuals were included in the analysis, 268 in WT and 149 in VOC groups respectively. In both groups MCA identified three similar clusters; a musculoskeletal (MSK) cluster characterised by joint pain and myalgia, a cardiorespiratory cluster and a less symptomatic cluster. Differences in characteristic symptoms were only seen in the cardiorespiratory cluster where a decrease in the frequency of palpitations (10% vs 34% p = 0.008) and an increase in cough (63% vs 17% p < 0.001) in the VOC compared to WT groups was observed. Analysis of the frequency of individual symptoms showed significantly lower frequency of both chest pain (25% vs 39% p = 0.004) and palpitations (12% vs 32% p < 0.001) in the VOC group compared to the WT group. In adjusted analysis being in the VOC group was significantly associated with a lower odds of both chest pain and palpitations, but vaccination was not associated with these symptoms.

Conclusion

This study suggests changes in long COVID phenotype in individuals infected later in the pandemic, with less palpitations and chest pain reported. Adjusted analyses suggest that these effects are mediated through introduction of variants rather than an effect from vaccination.
Understanding the lived experience of pregnancy and birth for survivors of rape and sexual assault
Authors: Lissmann Rebecca , Lokot Michelle , Marston Cicely
Published: November 2023

Background

One in five women in the UK are survivors of rape and sexual assault, and four in five women will give birth. This implies that a substantial number of women experience rape and sexual assault before pregnancy. We highlight and explore the voices and lived experiences of survivors during pregnancy and birth, to better understand the relationship between sexual violence, biomedicine, and pregnancy and to inform maternity care practice.

Methods

This qualitative research took an intersectional feminist approach. We conducted in-depth individual interviews in England with fourteen women who self-identified as survivors of rape or sexual assault, and who had experienced pregnancy and birth after the assault. We conducted open line-by-line coding of the interview transcripts, and identified key themes and sub-themes inductively.

Results

Three themes help summarise the narratives: control, safety and trauma. Maintaining a sense of control was important to survivors but they often reported objectification by healthcare staff and lack of consent or choice about healthcare decisions. Participants’ preferences for giving birth were often motivated by their desire to feel in control and avoid triggering traumatic memories of the sexual assault. Survivors felt safer when they trusted staff. Many participants said it was important for staff to know they were survivors but none were asked about this. Pregnancy and birth experiences were triggering when they mirrored the assault, for instance if the woman was prevented from moving. Many of our participants reported having unmet mental health care needs before, during or after pregnancy.

Conclusions

Survivors of sexual violence have specific maternity care needs. For our participants, these needs were often not met, leading to negative or traumatic experiences of pregnancy and birth. Systemic biases and poor birth experience jeopardise both psychological and physical safety. Funding for maternity and mental health services must be improved, so that they meet minimum staffing and care standards. Maternity services should urgently introduce trauma-informed models of care.