bluuebunny/crossref_metadata_2025 huggingface dataset:
Phenotypic and proteomic analysis of plasma extracellular vesicles highlights them as potential biomarkers of primary Sjögren syndrome
Authors: Ferrant Juliette , Pontis Adeline , Zimmermann François , Dingli Florent , Poullet Patrick , Loew Damarys , Tarte Karin , Dumontet Erwan
Published: July 2023

Sjögren syndrome (SjS) is an autoimmune disease characterized by the destruction of the exocrine gland epithelia, causing a dryness of mucosa called sicca symptoms, and whose main life-threatening complication is lymphoma. There is a need for new biomarkers in this disease, notably diagnostic biomarkers for patients with genuine sicca symptoms that do not meet current criteria, and prognostic biomarkers for patients at risk of lymphoma. Plasma extracellular vesicles (EVs) are promising biomarker candidates in several diseases, but their potential has not yet been explored in SjS. In this proof-of-concept study, we characterized EVs from primary SjS patients (pSS, n=12) at the phenotypic and proteomic levels, compared to EVs from healthy donor (HD, n=8) and systemic lupus erythematosus patients (SLE, n=12). Specific plasma EVs subpopulations, derived from neutrophils, endothelial, and epithelial cells, were found increased in pSS. We also identified a pSS proteomic signature in plasma EVs, including neutrophil-, epithelial-, and endothelial-related proteins, such as integrin alpha M (ITGAM), olfactomedin-4 (OLFM4), Ras-related protein RAB10, and CD36. Overall, our results support the relevance of plasma EVs as biomarkers in SjS.
A d-peptide-based oral nanotherapeutic modulates the PD-1/PD-L1 interaction for tumor immunotherapy
Authors: Liu Dan , Wang Jingmei , You Weiming , Ma Fang , Sun Qi , She Junjun , He Wangxiao , Yang Guang
Published: July 2023

Background

PD-1/PD-L1 immune checkpoint inhibitors are currently the most commonly utilized agents in clinical practice, which elicit an immunostimulatory response to combat malignancies. However, all these inhibitors are currently administered via injection using antibody-based therapies, while there is a growing need for oral alternatives.

Methods

This study has developed and synthesized exosome-wrapped gold–peptide nanocomplexes with low immunogenicity, which can target PD-L1 and activate antitumor immunity in vivo through oral absorption. The SuperPDL1exo was characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), and gel silver staining. The transmembrane ability of SuperPDL1exo was evaluated by flow cytometry and immunofluorescence. Cell viability was determined using the Cell Counting Kit-8 (CCK-8) assay. ELISA experiments were conducted to detect serum and tissue inflammatory factors, as well as serum biochemical indicators. Tissue sections were stained with H&E for the evaluation of the safety of SuperPDL1exo. An MC38 colon cancer model was established in immunocompetent C56BL/6 mice to evaluate the effects of SuperPDL1exo on tumor growth in vivo. Immunohistochemistry (IHC) staining was performed to detect cytotoxicity factors such as perforin and granzymes.

Results

First, SuperPDL1 was successfully synthesized, and milk exosome membranes were encapsulated through ultrasound, repeated freeze–thaw cycles, and extrusion, resulting in the synthesis of SuperPDL1exo. Multiple characterization results confirmed the successful synthesis of SuperPDL1exo nanoparticles. Furthermore, our data demonstrated that SuperPDL1exo exhibited excellent colloidal stability and superior cell transmembrane ability. In vitro and in vivo experiments revealed that SuperPDL1exo did not cause damage to multiple systemic organs, demonstrating its good biocompatibility. Finally, in the MC38 colon cancer mouse model, it was discovered that SuperPDL1exo could inhibit the progression of colon cancer, and this tumor-suppressive effect was mediated through the activation of tumor-specific cytotoxic T lymphocyte (CTL)-related immune responses.

Conclusion

This study has successfully designed and synthesized an oral nanotherapeutic, SuperPDL1exo, which demonstrates small particle size, excellent colloidal stability, transmembrane ability in tumor cells, and biocompatibility. In vivo experiments have shown that it effectively activates T-cell immunity and exerts antitumor effects.
The effects of sea-bottom plowing on phytoplankton assemblages: a case study of northern Hiroshima Bay, the Seto Inland Sea of Japan
Authors: Ohara Shizuka , Yano Ryoko , Furuya Kenichiro , Sato Takafumi , Ikeda Syunichiro , Koike Kazuhiko
Published: July 2023

Sea-bottom plowing is originally a method used to oxidize sediment by stirring the bottom with a trawl fishing tool, and its effect in increasing primary productivity of the water column was investigated in the western part of the Seto Inland Sea of Japan. Preliminary field sampling showed that diatom resting stage cells were abundant in the sediment of the tested area at 1.5–2.6 × 105 MPN g-1 wet sediment. When the sediment was added to filtered seawater, diatom cells emerged from the sediment after one day and increased more drastically under a light level corresponding to sunny weather than cloudy weather. In the actual trials of sea-bottom plowing on the field for a continuous period of 4 years, dissolved inorganic nutrients increased at the bottom layer after the plowing and promoted photosynthetic activities of the phytoplankton communities in 2018 and 2021. Chlorophyll a concentration at the middle layers increased 1.06–2.15 times after plowing throughout the trials for 4 years. Diatoms contributed to 67–99% of this chlorophyll a concentrations and included the genera Skeletonema and Chaetoceros, which formed resting-stage cells in the sediment. Pseudo-nitzschia spp. often increased after the plowing, which was assumed to be of seawater origin. Estimated primary productivities of the middle layers dropped once the following day due to turbid water caused by the plowing but increased 2.03–4.41 times after two or five days in 2018, 2019, and 2021. These results suggest that sea-bottom plowing has an enrichment effect on diatoms and could be a possible measure to fertilize the sea.
Combination of Chinese herbal medicine and conventional western medicine for coronavirus disease 2019: a systematic review and meta-analysis
Authors: Tong Lei , Ma Zhenyu , Zhou Yixiao , Yang Shuping , Yang Yalin , Luo Jingran , Huang Junbo , Wang Fucai
Published: July 2023

Objective

This study aimed to assess the efficacy and safety of Chinese herbal medicine (CHM) plus conventional western medicine (CWM) in comparison with CWM against COVID-19.

Methods

We searched eight electronic databases and three trial registers spanning from January 1, 2020 to May 18, 2023. We included randomized controlled trials (RCTs) comparing the effectiveness and safety of CHM plus CWM and CWM against COVID-19 in our study. The Cochrane Risk of Bias tool 2.0 (RoB2) was applied to evaluate the methodological quality of the included RCTs. The Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) system was employed to assess the certainty of evidence. Statistical analysis was implemented in R version 4.1.2.

Results

Our study included 50 RCTs involving 11,624 patients. In comparison with sole CWM, CHM plus CWM against COVID-19 significantly enhanced clinical effective rate (RR = 1.18, 95% CI [1.13, 1.22]), improved chest image (RR = 1.19, 95% CI [1.11, 1.28]), inhibited clinical deterioration (RR = 0.45, 95% CI [0.33, 0.60]), lowered mortality (RR = 0.53, 95% CI [0.40, 0.70]), and reduced the total score of TCM syndrome (SMD = −1.24, 95% CI [−1.82, −0.66]). SARS-CoV-2 nucleic acid conversion time (MD = −2.66, 95% CI [−3.88, −1.44]), duration of hospitalization (MD = −2.36, 95% CI [−3.89, −0.82]), and clinical symptom (fever, cough, fatigue, and shortness of breath) recovery times were shorter in CHM plus CWM groups than in CWM groups. Further, CHM plus CWM treatment was more conducive for some laboratory indicators returning to normal levels. No statistical difference was found in the incidence of total adverse reactions between the two groups (RR = 0.97, 95% CI [0.88, 1.07]). We assessed the risk of bias for 246 outcomes, and categorized 55 into “low risk”, 151 into “some concerns”, and 40 into “high risk”. Overall, the certainty of the evidence ranged from moderate to very low.

Conclusions

Potentially, CHM listed in this study, as an adjunctive therapy, combining with CWM is an effective and safe therapy mode for COVID-19. However, more high-quality RCTs are needed to draw more accurate conclusions.

Clinical trial registration

https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=293963.
Aberrations in ion channels interacting with lipid metabolism and epithelial–mesenchymal transition in esophageal squamous cell carcinoma
Authors: Parthasarathi K. T. Shreya , Mandal Susmita , George John Philip , Gaikwad Kiran Bharat , Sasidharan Sruthi , Gundimeda Seetaramanjaneyulu , Jolly Mohit Kumar , Pandey Akhilesh , Sharma Jyoti
Published: July 2023

Esophageal squamous cell carcinoma (ESCC) is the most prevalent malignant gastrointestinal tumor. Ion channels contribute to tumor growth and progression through interactions with their neighboring molecules including lipids. The dysregulation of membrane ion channels and lipid metabolism may contribute to the epithelial–mesenchymal transition (EMT), leading to metastatic progression. Herein, transcriptome profiles of patients with ESCC were analyzed by performing differential gene expression and weighted gene co-expression network analysis to identify the altered ion channels, lipid metabolism- and EMT-related genes in ESCC. A total of 1,081 differentially expressed genes, including 113 ion channels, 487 lipid metabolism-related, and 537 EMT-related genes, were identified in patients with ESCC. Thereafter, EMT scores were correlated with altered co-expressed genes. The altered co-expressed genes indicated a correlation with EMT signatures. Interactions among 22 ion channels with 3 hub lipid metabolism- and 13 hub EMT-related proteins were determined using protein–protein interaction networks. A pathway map was generated to depict deregulated signaling pathways including insulin resistance and the estrogen receptor-Ca2+ signaling pathway in ESCC. The relationship between potential ion channels and 5-year survival rates in ESCC was determined using Kaplan–Meier plots and Cox proportional hazard regression analysis. Inositol 1,4,5-trisphosphate receptor type 3 (ITPR3) was found to be associated with poor prognosis of patients with ESCC. Additionally, drugs interacting with potential ion channels, including GJA1 and ITPR3, were identified. Understanding alterations in ion channels with lipid metabolism and EMT in ESCC pathophysiology would most likely provide potential targets for the better treatment of patients with ESCC.
Probing the conformational changes of in vivo overexpressed cell cycle regulator 6S ncRNA
Authors: Makraki Eleni , Miliara Sophia , Pagkalos Michalis , Kokkinidis Michael , Mylonas Efstratios , Fadouloglou Vasiliki E.
Published: July 2023

The non-coding 6S RNA is a master regulator of the cell cycle in bacteria which binds to the RNA polymerase-σ70 holoenzyme during the stationary phase to inhibit transcription from the primary σ factor. Inhibition is reversed upon outgrowth from the stationary phase by synthesis of small product RNA transcripts (pRNAs). 6S and its complex with a pRNA were structurally characterized using Small Angle X-ray Scattering. The 3D models of 6S and 6S:pRNA complex presented here, demonstrate that the fairly linear and extended structure of 6S undergoes a major conformational change upon binding to pRNA. In particular, 6S:pRNA complex formation is associated with a compaction of the overall 6S size and an expansion of its central domain. Our structural models are consistent with the hypothesis that the resultant particle has a shape and size incompatible with binding to RNA polymerase-σ70. Overall, by use of an optimized in vivo methodological approach, especially useful for structural studies, our study considerably improves our understanding of the structural basis of 6S regulation by offering a mechanistic glimpse of the 6S transcriptional control.
Focal epilepsy without overt epileptogenic lesions: no evidence of microstructural brain tissue damage in multi-parametric quantitative MRI
Authors: Hamid Celona , Maiworm Michelle , Wagner Marlies , Knake Susanne , Nöth Ulrike , Deichmann Ralf , Gracien René-Maxime , Seiler Alexander
Published: July 2023

Background and purpose

In patients with epilepsies of structural origin, brain atrophy and pathological alterations of the tissue microstructure extending beyond the putative epileptogenic lesion have been reported. However, in patients without any evidence of epileptogenic lesions on diagnostic magnetic resonance imaging (MRI), impairment of the brain microstructure has been scarcely elucidated. Using multiparametric quantitative (q) magnetic resonance imaging MRI, we aimed to investigate diffuse impairment of the microstructural tissue integrity in MRI-negative focal epilepsy patients.

Methods

27 MRI-negative patients with focal epilepsy (mean age 33.1 ± 14.2 years) and 27 matched healthy control subjects underwent multiparametric qMRI including T1, T2, and PD mapping at 3 T. After tissue segmentation based on synthetic anatomies, mean qMRI parameter values were extracted from the cerebral cortex, the white matter (WM) and the deep gray matter (GM) and compared between patients and control subjects. Apart from calculating mean values for the qMRI parameters across the respective compartments, voxel-wise analyses were performed for each tissue class.

Results

There were no significant differences for mean values of quantitative T1, T2, and PD obtained from the cortex, the WM and the deep GM between the groups. Furthermore, the voxel-wise analyses did not reveal any clusters indicating significant differences between patients and control subjects for the qMRI parameters in the respective compartments.

Conclusions

Based on the employed methodology, no indication for an impairment of the cerebral microstructural tissue integrity in MRI-negative patients with focal epilepsy was found in this study. Further research will be necessary to identify relevant factors and mechanisms contributing to microstructural brain tissue damage in various subgroups of patients with epilepsy.
Using MemTrax memory test to screen for post-stroke cognitive impairment after ischemic stroke: a cross-sectional study
Authors: Zhao Xiaoxiao , Dai Shujuan , Zhang Rong , Chen Xinjie , Zhao Mingjie , Bergeron Michael F. , Zhou Xianbo , Zhang Junyan , Zhong Lianmei , Ashford J. Wesson , Liu Xiaolei
Published: July 2023

Background

Whereas the Montreal Cognitive Assessment (MoCA) and Addenbrooke’s cognitive examination-revised (ACE-R) are commonly used tests for the detection of post-stroke cognitive impairment (PSCI), these instruments take 10–30 min to administer and do not assess processing speed, which is a critical impairment in PSCI. MemTrax (MTx) is a continuous recognition test, which evaluates complex information processing, accuracy, speed, and attention, in 2 min.

Aim

To evaluate whether MTx is an effective and practical tool for PSCI assessment.

Methods

This study enrolled acute ischemic stroke (AIS) patients who have assessed the cognitive status including MTx, clinical dementia rating (CDR), MoCA, Neuropsychiatric Inventory (NPI), Hamilton depression scale (HAMD), Hamilton anxiety scale (HAMA), the National Institute of Health Stroke Scale (NIHSS), modified Rankin scale (mRS), and Barthel Index of activity of daily living (BI) combined with the physical examinations of the neurologic system at the 90-day (D90) after the AIS. The primary endpoint of this study was establishing MTx cut-offs for distinguishing PSCI from AIS.

Results

Of the 104 participants, 60 were classified to the PSCI group. The optimized cut-off value of MTx-%C (percent correct) was 78%, with a sensitivity and specificity for detecting PSCI from Non-PSCI of 90.0 and 84.1%, respectively, and an AUC of 0.919. Regarding the MTx-Cp (Composite score = MTx-%C/MTx-RT), using 46.3 as a cut-off value, the sensitivity and specificity for detecting PSCI from Non-PSCI were 80.0 and 93.2%, with an AUC of 0.925. Multivariate linear regression showed that PSCI reduced the MTx-%C (Coef. −14.18, 95% CI −18.41∼−9.95, p < 0.001) and prolonged the MTx-RT (response time) (Coef. 0.29, 95% CI 0.16∼0.43, p < 0.001) and reduced the MTx-CP (Coef. −19.11, 95% CI −24.29∼−13.93, p < 0.001).

Conclusion

MemTrax (MTx) is valid and effective for screening for PSCI among target patients and is a potentially valuable and practical tool in the clinical follow-up, monitoring, and case management of PSCI.
Normative uncertainty and societal preferences: the problem with evaluative standards
Authors: Kuilman Sietze Kai , Andriamahery Koji , Jonker Catholijn M. , Siebert Luciano Cavalcante
Published: July 2023

Many technological systems these days interact with their environment with increasingly little human intervention. This situation comes with higher stakes and consequences that society needs to manage. No longer are we dealing with 404 pages: AI systems today may cause serious harm. To address this, we wish to exert a kind of control over these systems, so that they can adhere to our moral beliefs. However, given the plurality of values in our societies, which “oughts” ought these machines to adhere to? In this article, we examine Borda voting as a way to maximize expected choice-worthiness among individuals through different possible “implementations” of ethical principles. We use data from the Moral Machine experiment to illustrate the effectiveness of such a voting system. Although it appears to be effective on average, the maximization of expected choice-worthiness is heavily dependent on the formulation of principles. While Borda voting may be a good way of ensuring outcomes that are preferable to many, the larger problems in maximizing expected choice-worthiness, such as the capacity to formulate credences well, remain notoriously difficult; hence, we argue that such mechanisms should be implemented with caution and that other problems ought to be solved first.
Factors that predict weight loss success differ by diet intervention type
Authors: Losavio Jordan , Keenan Michael J. , Gollub Elizabeth A. , Silver Heidi J.
Published: July 2023

Background

Many types of diet intervention can achieve negative energy balance and successful weight loss in persons with obesity. However, within any dietary strategy, there is large inter-individual variation in the weight loss response. The aim of this study is to determine factors that predict weight loss success for diet interventions that vary by macronutrient and caloric composition.

Methods

Participants with BMI 30.0 to 49.9 kg/m2 self-selected one of three diet intervention trials for weight loss: low carbohydrate (LOW CHO), low fat (LOW FAT), or low calorie (LOW KCAL). Multivariable regression models were developed to determine the significance of predictor demographic, body composition, metabolic, clinical, and dietary variables for each diet type.

Results

Weight loss over 12–16 weeks averaging −5.1 ± 4.0 kg from baseline weight, p < 0.001, was not significantly different among diet types. Several different factors were identified that account for the inter-individual variance in weight loss success. Regardless of diet type, the most robust predictor of weight loss success was completion of the intervention, accounting for 20–30% of the variance. Factors predicting diet intervention completion were age, physical activity level, blood leptin level, blood pressure, and the amount of weight loss occurring. Differences by diet type in cardiometabolic risk factor reduction were identified with LOW CHO decreasing glycemia/insulinemia factors, LOW FAT decreasing lipidemia factors, and LOW KCAL decreasing inflammatory factors.

Conclusion

These data provide evidence to inform more precise and personalized approaches to diet intervention for weight loss and cardiometabolic health.
Severe congenital neutropenia due to jagunal homolog 1 (JAGN1) mutation: a case report and literature review
Authors: Thomas Sanya , Guenther Geoffrey , Rowe Jared H. , Platt Craig D. , Shimamura Akiko , Levy Ofer , Ganapathi Lakshmi
Published: July 2023

Severe congenital neutropenia caused by jagunal homolog 1 (JAGN1) mutation is a rare condition resulting from maturation arrest secondary to endoplasmic reticulum stress response from impaired neutrophil protein glycosylation. Here, we report a case of a 4-year-old boy who presented with a history of recurrent infections and manifestations, including recurrent intracranial hemorrhage. A review of similar cases reported in the literature indicates that a bleeding diathesis has not been previously described in these patients. We hypothesize that this newly described association of bleeding complications in this patient with JAGN1 mutation is secondary to defective glycosylation in the normal functioning of platelets or clotting factors. Recurrent infections with intracranial hemorrhage, new focal neurologic defects, or altered mental status in a child should warrant a suspicion for this immunodeficiency for the prompt initiation of treatment and prophylaxis for life-threatening infections or trauma.
T-type calcium channel modulation by hydrogen sulfide in neuropathic pain conditions
Authors: Rangel-Galván Maricruz , Rangel-Galván Violeta , Rangel-Huerta Alejandro
Published: July 2023

Neuropathic pain can appear as a direct or indirect nerve damage lesion or disease that affects the somatosensory nervous system. If the neurons are damaged or indirectly stimulated, immune cells contribute significantly to inflammatory and neuropathic pain. After nerve injury, peripheral macrophages/spinal microglia accumulate around damaged neurons, producing endogenous hydrogen sulfide (H2S) through the cystathionine-γ-lyase (CSE) enzyme. H2S has a pronociceptive modulation on the Cav3.2 subtype, the predominant Cav3 isoform involved in pain processes. The present review provides relevant information about H2S modulation on the Cav3.2 T-type channels in neuropathic pain conditions. We have discussed that the dual effect of H2S on T-type channels is concentration-dependent, that is, an inhibitory effect is seen at low concentrations of 10 µM and an augmentation effect on T-current at 100 µM. The modulation mechanism of the Cav3.2 channel by H2S involves the direct participation of the redox/Zn2+ affinity site located in the His191 in the extracellular loop of domain I of the channel, involving a group of extracellular cysteines, comprising C114, C123, C128, and C1333, that can modify the local redox environment. The indirect interaction pathways involve the regulation of the Cav3.2 channel through cytokines, kinases, and post-translational regulators of channel expression. The findings conclude that the CSE/H2S/Cav3.2 pathway could be a promising therapeutic target for neuropathic pain disorders.
The effect of auricular vagus nerve stimulation on electroencephalography and electromyography measurements in healthy persons
Authors: Konakoğlu Gülşah , Özden Ali Veysel , Solmaz Hakan , Bildik Celaleddin
Published: July 2023

Objectives: Auricular vagus nerve stimulation (VNS) is a non-invasive treatment modality. Opinions that it can be used in the treatment of various clinical problems have gained importance in recent years. In this study, it was aimed to lay the groundwork for the use of the auricular VNS in different ears.

Methods: Healthy individuals (n = 90) were divided into three groups: unilateral left (n = 30), unilateral right (n = 30), and bilateral (n = 30) auricular VNS. Electroencephalography (EEG) and electromyography (EMG) measurements were performed before and after auricular VNS (10 Hz, 300 µs, 20 min) for a single session.

Results: An increase in wrist extensor muscles activation was detected on the contralateral side of the auricular VNS application side. It has been observed that there is a general decrease in the power of high-frequency waves and an increase in the power of lower-medium frequency waves in various parts of the brain.

Conclusion: Our findings suggest that the projection of the auricular VNS in the central nervous system may also affect the corticospinal tracts.
Development of InDels markers for the identification of cytoplasmic male sterility in Sorghum by complete chloroplast genome sequences analysis
Authors: Choe Myeong-Eun , Kim Ji-Young , Syed Nabi Rizwana Begum , Han Sang-Ik , Cho Kwang-Soo
Published: July 2023

Cytoplasmic male sterility (CMS) is predominantly used for F1 hybrid breeding and seed production inSorghum. DNA markers to distinguish between normal fertile (CMS-N) and sterile (CMS-S) male cytoplasm can facilitate F1 hybrid cultivar development inSorghumbreeding programs. In this study, the complete chloroplast (cp) genome sequences of CMS-S and KoreanSorghumcultivars were obtained using next-generation sequencing. Thede novoassembled genome size of ATx623, the CMS-S line of the chloroplast, was 140,644bp. When compared to the CMS–S and CMS-N cp genomes, 19 single nucleotide polymorphisms (SNPs) and 142 insertions and deletions (InDels) were identified, which can be used for marker development for breeding, population genetics, and evolution studies. Two InDel markers with sizes greater than 20 bp were developed to distinguish cytotypes based on the copy number variation of lengths as 28 and 22 bp tandem repeats, respectively. Using the newly developed InDel markers with five pairs of CMS-S and their near isogenic maintainer line, we were able to easily identify their respective cytotypes. The InDel markers were further examined and applied to 1,104 plants from six KoreanSorghumcultivars to identify variant cytotypes. Additionally, the phylogenetic analysis of sevenSorghumspecies with complete cp genome sequences, including wild species, indicated that CMS-S and CMS-N containedMiloandKafircytotypes that might be hybridized fromS. propinquumandS. sudanese, respectively. This study can facilitate F1 hybrid cultivar development by providing breeders with reliable tools for marker-assisted selection to breed desirableSorghumvarieties.
Comparative cytological and transcriptome analyses of ny2 mutant delayed degeneration of tapetal cells and promotes abnormal microspore development in neo-tetraploid rice
Authors: Kamara Nabieu , Jiao Yamin , Huang Weicong , Cao Lichong , Zhu Lianjun , Zhao Chongchong , Huang Xu , Shivute Fimanekeni Ndaitavela , Liu Xiangdong , Wu Jinwen , Shahid Muhammad Qasim
Published: July 2023

We aimed to investigate the genetic defects related to pollen development and infertility in NY2, a novel tetraploid rice germplasm known as Neo-tetraploid rice. This rice variety was created through the crossbreeding and selective breeding of various autotetraploid rice lines and has previously shown high fertility. Our previous research has revealed that the NY2 gene, encoding a eukaryotic translation initiation factor 3 subunit E, regulates pollen fertility. However, the underlying mechanism behind this fertility is yet to be understood. To shed light on this matter, we performed a combined cytological and transcriptome analysis of the NY2 gene. Cytological analysis indicated that ny2 underwent abnormal tapetal cells, microspore, and middle layer development, which led to pollen abortion and ultimately to male sterility. Genetic analysis revealed that the F1 plants showed normal fertility and an obvious advantage for seed setting compared to ny2. Global gene expression analysis in ny2 revealed a total of 7545 genes were detected at the meiosis stage, and 3925 and 3620 displayed upregulation and downregulation, respectively. The genes were significantly enriched for the gene ontology (GO) term “carbohydrate metabolic process. Moreover, 9 genes related to tapetum or pollen fertility showed down-regulation, such as OsABCG26 (ATP Binding Cassette G26), TMS9-1 (Thermosensitive Male Sterility), EAT1 (Programmed cell death regulatory), KIN14M (Kinesin Motor), OsMT1a (Metallothionein), and OsSTRL2 (Atypical strictosidine synthase), which were validated by qRT-PCR. Further analyses of DEGs identified nine down-regulated transcription factor genes related to pollen development. NY2 is an important regulator of the development of tapetum and microspore. The regulatory gene network described in this study may offer important understandings into the molecular processes that underlie fertility control in tetraploid rice.
Meaning in life as a protective factor against depression
Authors: Baquero-Tomás Marina , Grau Mª Dolores , Moliner Adoración-Reyes , Sanchis-Sanchis Alejandro
Published: July 2023

The main objective of this study was to analyze the mediating role that meaning in life has between emotion dysregulation and depressive symptomatology in Spanish university students. Five hundred and sixty-six Spanish university students participated in the study. All of them completed the Difficulties in Emotion Regulation Scale (DERS), the Patient Health Questionnaire (PHQ-9) and the Purpose in Life Test (PIL-10). A mediation model was performed to test the hypothesis that meaning in life mediates the effect of emotional dysregulation on depressive symptomatology in college students. The results show a positive, moderate, and statistically significant correlation between depression and emotional dysregulation. In addition, a negative, moderate, and statistically significant correlation was found between depression and meaning in life, and a negative, small, and statistically significant correlation between emotional dysregulation and meaning in life. Finally, the results of the mediation model evidence the role of meaning in life in different emotional dysregulation strategies and depressive symptomatology. These findings suggest the importance of incorporating the meaning in life variable in the development and implementation of prevention and treatment programs for psychological disorders.
Anger and psychological symptoms relationship: mediator role of maladaptive schemas
Authors: Özmen Ahmet
Published: July 2023

Trait anger is the strong predictor of various psychological symptoms like anxiety, depression, and hostility. Explaining how and why this relationship occurs is crucial to come up with more effective prevention and intervention strategies in the field. To this end, the current study aimed to reveal the mediating effect of early maladaptive schemas, which is the basic concept of schema therapy, on the relations of trait anger and psychological symptoms. Data was collected from 301 university students by using the measurement tools of Brief Symptom Inventory, Trait Anger Scale and Young Schema Scale. Findings revealed that trait anger positively predicted psychological symptoms. Secondly, a set of predictive models were prepared to detect the mediating effect of early maladaptive schemas. According to the analysis in the last stage, early maladaptive schemas fully mediated the relationship between trait anger and psychological symptoms.
The role of the body in the experience of installation art: a case study of visitors' bodily, emotional, and transformative experiences in Tomás Saraceno's “in orbit”
Authors: Kühnapfel Corinna , Fingerhut Joerg , Pelowski Matthew
Published: July 2023

Installation art, with its immersive and participatory character, has been argued to require the use and awareness of the body, which potentially constitute key parts of the artwork's experience and appreciation. Heightened body awareness is even argued to be a key to particularly profound emotional or even transformative states, which have been frequently ascribed to this genre. However, the body in the experience of installation art has rarely been empirically considered. To address this gap, we investigated the body's role in the experience of Tomás Saraceno's in orbit installation. Based on a list of self-report items created from a review of the theoretical literature, we—for the first time—captured (quantitatively and qualitatively): what kind of subjective bodily experiences visitors (N = 230) reported, how these items grouped into clusters (using network science), and how these relate to emotion, art appraisal, and transformative outcomes. Network analysis of the items determined four communities related to “interoception,” “presence,” “disturbance,” and “proprioception.” Proprioception (e.g., awareness of balance/movement/weight) turned out to be a significant determinant of art appreciation in our study, and, together with “disturbing” body experiences (feeling awkward/watched/chills), coincided with transformation. We also assessed individual differences in body awareness yet did not find that these moderate those relationships. We suggest future research on installation art based on a more unified assessment of the role of the body in embodied-enactive aesthetics and its relation to the intensity and impact of art experience in general.
#EntreViagenseAprendizagens: study protocol of a school-based intervention to promote well-being and healthy lifestyles among adolescents
Authors: Francisco Rita , Raposo Beatriz , Hormigo Mafalda , Sesifredo Mónica , Carvalho Ana , Justo Ana , Godinho Cristina Albuquerque
Published: July 2023

Background

Adolescence is a critical period of development in which well-being usually decreases, mental health problems (e.g., depression, anxiety) increase, and lifestyles become less healthy. Schools are a primary setting for the promotion of the well-being and overall health of adolescents, and preventive actions should be a priority within the scope of health-promoting schools. #EntreViagenseAprendizagens is a school-based intervention aiming to promote well-being and healthy lifestyles among adolescents based on social and emotional learning, positive psychology, and health education approaches.

Methods

This protocol describes a school-based intervention, #EntreViagenseAprendizagens, that will be implemented in several schools in Portugal. The program is aimed at 8th and 9th grade students (14–16 years old) and comprises 20 weekly sessions. One of the sessions is aimed at the students’ parents/guardians. The intervention content targets social and emotional skills, health literacy (physical and mental health), healthy lifestyles, character strengths, and well-being. An experimental design will be used in the intervention evaluation. Eighth grade classes will be randomly assigned to the intervention group or the control group. All students complete the same assessment protocol at baseline, post-intervention, and 9-month follow-up. The impact assessment protocol includes measures related to well-being, health literacy, health-related knowledge, attitudes and behaviors, relationships with others, social and emotional skills, and sociodemographic data. Process evaluation includes evaluation forms at the end of each session and at the end of the program and focus groups with students, parents, and teachers at the end of the program.

Discussion

This school-based intervention may play an important role in promoting students’ well-being and in preventing unhealthy lifestyles and socio-emotional maladjustment, by focusing on the development of social and emotional skills and health literacy among adolescents, empowering them to face the changing future and grow up healthy. Furthermore, this project aims to provide relevant scientific findings that can contribute to the development of better health-promoting schools.
Effects of use motivations and alexithymia on smartphone addiction: mediating role of insecure attachment
Authors: Jin Xinquan , Jiang Qiang , Xiong Weiyan , Zhao Wei
Published: July 2023

Background and objectives

Concern exists regarding the potential negative consequences of smartphone addiction among adolescents. This study investigated the effect of use motivations and alexithymia on smartphone addiction among adolescents with two insecure attachment styles, namely, anxious and avoidant attachment. These attachment styles were regarded as mediating variables.

Methods

Self-report measures were used to assess use motivations, alexithymia, insecure attachment and smartphone addiction. Data were collected from 748 junior high school students (382 males and 366 females) in northeastern China. Structural equation modeling was used to test our hypothesis that use motivations and alexithymia are directly associated with smartphone addiction and also through the mediation of attachment insecurities.

Results

The structural equation modeling results showed a strong and positive correlation between use motivation and smartphone addiction, with avoidant attachment mediating such a relationship. Meanwhile, the two components of alexithymia, difficulty identifying feelings and externally oriented thinking, positively predicted smartphone addiction, with avoidant attachment serving as a mediator of this effect. In addition, the mediation analysis results demonstrated that attachment anxiety mediated the connection between escape drive, extrinsically focused thought, and smartphone addiction.

Conclusion

Findings describe how attachment insecurities, smartphone use motivations, and alexithymia can interact with one another to predict smartphone addiction. Smartphone use motivation types and alexithymia symptoms should be taken into consideration when designing targeted intervention programs for smartphone addiction to address the different attachment needs of adolescents, which would be helpful to reduce their smartphone addiction behaviors.
The genesis of perinatal clinical psychology and its contemporary issues
Authors: Missonnier Sylvain
Published: July 2023

The main aim of French clinical psychology is to explain the psychic processes of transformation, to which the subject is central. In this context, transformations in the perinatal period open an innovating field in perinatal clinical psychology focused on the conscious/subconscious, subjective/inter-subjective psychic reality of a subject who is in the process of becoming (or becoming once again) a parent and being born a human.
Hematochezia caused by tandospirone in a patient with major depressive disorder and anxious distress: a case report
Authors: Jiang Xingmei , Ma Zhongrui , Li Zhixiong , Ou Ying , Luo Zhenhua , Li Zhe
Published: July 2023

Background

Major depressive disorder (MDD) with anxious distress is a relatively common condition that is often associated with a poor treatment response. In order to enhance the effectiveness of MDD treatment, 5-HT1A agonists like tandospirone are often prescribed in conjunction with antidepressants. While it is known that antidepressants can increase the risk of bleeding, whether tandospirone poses a similar risk remains uncertain.

Case presentation

We presented the case of a 55-year-old Chinese woman diagnosed with MDD and anxious distress. After receiving various types of antidepressants, she experienced hematochezia following the administration of tandospirone, sertraline, and agomelatine. The occurrence of hematochezia ceased after tandospirone was discontinued. The patient was subsequently discharged with a treatment regime consisting of sertraline and agomelatine. During the 1-month follow-up, she reported no hematochezia.

Conclusion

Tandospirone may potentially increase the risk of hematochezia in patients with MDD and anxious distress.
Hospital preparedness for COVID-19 in Indonesia
Authors: Lestari Fatma , Kadir Abdul , Puspitasari Attika , Suparni  , Wijaya Oktomi , EL-Matury Herlina J. , Liana Duta , Sunindijo Riza Yosia , Yani Hamid Achir , Azzahra Fira
Published: July 2023

Introduction

As a disaster-prone country, hospital preparedness in dealing with disasters in Indonesia is essential. This research, therefore, focuses specifically on hospital preparedness for COVID-19 in Indonesia, which is important given the indication that the pandemic will last for the foreseeable future.

Methods

During March to September 2022, a cross-sectional approach and a quantitative study was conducted in accordance with the research objective to assess hospital preparedness for the COVID-19 pandemic. This research shows the level of readiness based on the 12 components of the rapid hospital readiness checklist for COVID-19 published by the World Health Organization (WHO). Evaluators from 11 hospitals in four provinces in Indonesia (Capital Special Region of Jakarta, West Java, Special Region of Yogyakarta, and North Sumatra) filled out the form in the COVID-19 Hospital Preparedness Information system, which was developed to assess the level of hospital readiness.

Results

The results show that hospitals in Capital Special Region of Jakarta and Special Region of Yogyakarta have adequate level (≥ 80%). Meanwhile, the readiness level of hospitals in West Java and North Sumatra varies from adequate level (≥ 80%), moderate level (50% – 79%), to not ready level (≤ 50%).

Conclusion

The findings and the methods adopted in this research are valuable for policymakers and health professionals to have a holistic view of hospital preparedness for COVID-19 in Indonesia so that resources can be allocated more effectively to improve readiness.
Socio-geographical disparities of obesity and excess weight in adults in Spain: insights from the ENE-COVID study
Authors: Gutiérrez-González Enrique , García-Solano Marta , Pastor-Barriuso Roberto , Fernández de Larrea-Baz Nerea , Rollán-Gordo Almudena , Peñalver-Argüeso Belén , Peña-Rey Isabel , Pollán Marina , Pérez-Gómez Beatriz ,   the ENE-COVID Study Group
Published: July 2023

Background

In Spain, differences in the prevalence of obesity and excess weight according to sex and sociodemographic factors have been described at the national level, although current data do not allow to delve into geographical differences for these conditions. The aim was to estimate national and regional prevalences of adult obesity and excess weight in Spain by sex and sociodemographic characteristics, and to explore difference sources of inequalities in its distribution, as well as its geographical pattern.

Method

ENE-COVID study was a nationwide representative seroepidemiological survey with 57,131 participants. Residents in 35,893 households were selected from municipal rolls using a two-stage random sampling stratified by province and municipality size (April–June 2020). Participants (77.0% of contacted individuals) answered a questionnaire which collected self-reported weight and height, as well as different socioeconomic variables, that allowed estimating crude and standardized prevalences of adult obesity and excess weight.

Results

Crude prevalences of obesity and excess weight were higher in men (obesity: 19.3% vs. 18.0%; excess weight: 63.7% vs. 48.4%), while severe obesity was more prevalent in women (4.5% vs. 5.3%). These prevalences increased with age and disability, and decreased with education, census tract income and municipality size. Differences by educational level, relative census income, nationality or disability were clearly higher among women. Obesity by province ranged 13.3–27.4% in men and 11.4–28.1% in women; excess weight ranged 57.2–76.0% in men and 38.9–59.5% in women. The highest prevalences were located in the southern half of the country and some north-western provinces. Sociodemographic characteristics only explained a small part of the observed geographical variability (25.2% obesity).

Conclusion

Obesity and overweight have a high prevalence in Spain, with notable geographical and sex differences. Socioeconomic inequalities are stronger among women. The observed geographical variability suggests the need to implement regional and local interventions to effectively address this public health problem.
Knowledge, attitude, and practice of medication therapy management: a national survey among pharmacists in Indonesia
Authors: Rendrayani Farida , Alfian Sofa Dewi , Wahyudin Wawan , Puspitasari Irma Melyani
Published: July 2023

Introduction

The use of medication therapy management (MTM) is a proven method for reducing medication errors. MTM services rely heavily on pharmacists as service providers, particularly in community health centers (CHCs). Thus, understanding the knowledge, attitudes, and practices (KAP) of MTM among pharmacists in CHCs is crucial to the strategy for the implementation of MTM program in Indonesia. This study aimed to assess the level of KAP regarding MTM among pharmacists working at CHCs and its associated factors and investigate pharmacists’ perceptions of the barriers and facilitators of MTM provision in the future.

Methods

A cross-sectional online survey was conducted. The respondents were pharmacists working at CHCs in 28 provinces in Indonesia. Descriptive statistics were used to summarize the responses. Demographic differences were determined using Chi-square and Kruskal–Wallis tests, and associations were identified using multivariable ordinal regression for knowledge and multivariable logistic regression for attitude and practice. Barriers and facilitators were determined from codes and categories of frequency derived from pharmacists’ responses to the open-ended questions.

Results

Of the 1,132 pharmacists, 74.9% had a high level of knowledge, 53.6% had a positive attitude, and 57.9% had a positive practice toward MTM. Gender, practice settings, province of CHCs, years of practice, and experience in MTM services were factors associated with the KAP level. Respondents perceived that the chronic disease conditions in Indonesia, MTM service features, and current practices were facilitators of MTM provision. The lack of interprofessional collaboration, staff, pharmacist knowledge, patient cooperation, facilities/drug supply/documentation systems, stakeholder support, and patient compliance were the most common barriers to MTM implementation in the future.

Conclusion

Most of the pharmacists had high knowledge of MTM; however, only half had positive attitudes and practices toward MTM. Information about factors associated with the KAP level suggests that direct involvement is essential to improve pharmacists’ understanding and view of MTM. Pharmacists also perceived barriers to the MTM provision in the future, such as interprofessional and pharmacist-patient relationships. A training program is needed to improve the KAP of MTM and develop skills for collaborating with other healthcare professionals and communicating with patients.
Research on influencing factors of cultivated land productivity of high-standard farmland projects in Hanzhong city of China – an empirical study based on PLS-SEM
Authors: Dong Hao , Han Jichang , Zhang Yang , Chen Tianqing , Fan Hongjian , Wang Chuangjiang
Published: July 2023

Based on 295 capacity monitoring sites in Hanzhong city, we used partial least squares structural equation model to construct a model of the factors influencing cultivated land productivity using soil nutrients, farmland water resources, geological characteristics and soil properties as latent variables to explore the main factors affecting cultivated land productivity in Hanzhong city. The research results show that: (1) Soil nutrients, farmland water conservancy and soil properties were the main factors influencing cropland productivity, and had direct or indirect positive effects on cropland productivity, with the total effects ranging from soil properties (0.587) > farmland water conservancy (0.552) > soil nutrients (0.464). (2) Geological characteristics had no direct effect on cropland productivity, but indirectly influenced cropland productivity by affecting soil nutrients. (3) Slope had a negative effect on cropland productivity. Cultivated land productivity in Hanzhong city is influenced by various factors. Integrating the interactions and influence effects among the influencing factors, it is recommended to achieve coordinated and sustainable regional food production by improving water conservancy facilities, strengthening farmland quality, improving agricultural product quality, and building healthy capacity of cultivated land.