bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Microalgae Diets on the Biological and Growth Parameters of Oithona nana (Copepoda: Cyclopoida)
Authors: Huanacuni Jordan I. , Pepe-Victoriano Renzo , Lora-Vilchis María C. , Merino Germán E. , Torres-Taipe Fressia G. , Espinoza-Ramos Luis A.
Published: December 2021

Several species of the planktonic free-living genus Oithona have been successfully used in the larviculture of marine fish and shrimp. However, few studies have been published that allow us to estimate the potential of Oithona nana culture under controlled conditions. This work evaluated the effect of the microalgae Isochrysis galbana and Chaetoceros calcitrans as single (200,000 cells/mL) and mixed diets (100,000 + 100,000 cells/mL) on population and individual growth, ingestion rate, number of spawnings, fertility, development time by stage, and sex ratio of O. nana. We cultured this copepod at 28 ± 0.5 °C, 35 PSU salinity, 125 lux, and 12:12 photoperiod. Results showed that diet had no effect on the final population level (6273–7966 ind/L) or on individual growth, nor on sex ratio, with less males than females. With C. calcitrans, O. nana had a higher filtration rate (57 ng C/ind/day). On the other hand, a mixed diet induced a higher number of spawns (0.4 events/day) and nauplii per spawn (23 ind). Similarly, a single or mixed diet, containing I. galbana, accelerated the development rate by 6.33–7.00 days. We concluded that O. nana can be cultured with both microalgae, indicating its potential use in an intensive system for production. However, more research is required to improve the productivity of O. nana rearing.
Effects of Microencapsulated Methionine on Milk Production and Manure Nitrogen Excretions of Lactating Dairy Cows
Authors: King Layla , Wickramasinghe Janaka , Dooley Brooke , McCarthy Carrie , Branstad Emily , Grilli Ester , Baumgard Lance , Appuhamy Ranga
Published: December 2021

The study objective was to determine the effects of rumen-protected methionine (Met) by microencapsulation (RPM) on amino acid (AA) supply to the udder, milk production, and manure nitrogen (N) losses of dairy cows. A corn and soybean-based diet deficient in metabolizable Met (~10 g/d) was supplemented with RPM providing 0, 11.0, 19.3, and 27.5 g/d of Met. Dry matter intake (DMI), milk production, plasma essential AA (EAA), mammary plasma flow (MPF), and fecal (FN) and urinary N (UN) outputs (g/d) were determined. The RPM increased linearly milk yield, milk protein yield, and energy corrected milk yield (p < 0.040) without affecting DMI. Milk protein yield increased by 50 g/d for the 19.3 vs. 0 g/d dose (p = 0.006) but the rate of increment decreased for 27.5 g/d dose. Plasma Met, and MPF increased linearly with RPM dose (p < 0.050). Apparent total tract digestibility of crude protein (p = 0.020) and FN (p = 0.081) decreased linearly with RPM. The UN did not change but total manure N decreased linearly with RPM (p = 0.054). The RPM (19.3 g/d) seemed to help cows overcome the metabolizable Met deficiency while mitigating manure N excretions to the environment.
Effects of Bulk Tank Milk, Waste Milk, and Pasteurized Waste Milk on the Intake, Ruminal Parameters, Blood Parameters, Health, and Performance of Dairy Calves
Authors: Vieira Sabrina de Freitas , Coelho Sandra Gesteira , Diniz Neto Hilton do Carmo , Sá Hemily Cristina Menezes de , Pereira Barbara Pironi , Albuquerque Bianca Souza Ferreira , Machado Fernanda Samarini , Pereira Luiz Gustavo Ribeiro , Tomich Thierry Ribeiro , Renhe Isis Rodrigues Toledo , Campos Mariana Magalhães
Published: December 2021

The aim of this study was to evaluate the effects of bulk tank milk (BTM), WM, and PWM on the intake, ruminal parameters, blood parameters, health, and performance of dairy calves. Forty-five male crossbred dairy calves (Gyr × Holstein) were used. On their fourth day of age, animals were grouped according to body weight, serum protein levels, and genetic composition. Three treatments were assessed: BTM (n = 15), WM from cows in antibiotic treatment (n = 15), and PWM via high-temperature, short-time pasteurization (72–74 °C for 16 s) (n = 15). During the experimental period (from 4 to 60 d of age), animals were fed 6 L of milk/d, divided into two equal meals. Water and concentrate were provided ad libitum. Daily measurements were made for milk, concentrate, and water intakes, as well as for fecal and respiratory scores. Rumen fluid and blood were sampled weekly. The following parameters were evaluated: volatile fatty acids (VFAs), pH and ammonia-N in rumen fluid, and β-hydroxybutyrate (BHB) and glucose in blood. Animals were weighed at birth, 4 d of age, and weekly up to 60 d of age. At the end of the experimental period (60 ± 1 d), all animals were euthanized for pulmonary evaluation. The randomized complete design with an interaction between treatment and week was the experimental method of choice for testing the hypothesis of the treatment’s effect on all evaluated outcomes. Animals in the BTM treatment had higher milk dry matter intake (DMI), followed by WM and PWM calves. Concentrate DMI was lower for BTM in comparison to WM and PWM calves. However, total DMI showed no significant differences between treatments. The rumen fluid from calves receiving PWM had higher concentrations of acetate and propionate than that of BTM and WM animals. No differences were observed between treatments for blood glucose and BHB concentrations. Health parameters (fecal and respiratory scores) and pneumonia occurrence showed no significant difference between treatments. No differences were observed for average daily gain (ADG) or body growth. Feeding WM and PWM did not show significant negative effects on the intake, ruminal parameters, blood parameters, health, or performance of dairy calves.
Determination of the Nutritional Value of Diet Containing Bacillus subtilis Hydrolyzed Feather Meal in Adult Dogs
Authors: Machado Geruza Silveira , Correa Ana Paula Folmer , Pires Paula Gabriela da Silva , Marconatto Letícia , Brandelli Adriano , Kessler Alexandre de Mello , Trevizan Luciano
Published: December 2021

Feathers are naturally made up of non-digestible proteins. Under thermal processing, total tract digestibility can be partially improved. Furthermore, Bacillus subtilis (Bs) has shown a hydrolytic effect In vitro. Then, a Bs FTC01 was selected to hydrolyze enough feathers to produce a meal, and then test the quality and inclusion in the dog’s diet to measure the apparent total tract digestibility coefficient (ATTDC) in vivo and the microorganism’s ability to survive in the gastrointestinal tract. A basal diet was added with 9.09% hydrolyzed Bs feather meal (HFMBs) or 9.09% thermally hydrolyzed feather meal (HFMT). Nine adult dogs were randomized into two 10-day blocks and fed different diets. Microbial counts were performed on feather meal, diets and feces. The Bs was less effective in digesting the feathers, which reduced the ATTDC of dry matter, crude protein, energy and increased the production of fecal DM, but the fecal score was maintained (p > 0.05). The digestible energy of HFMT and HFMBs was 18,590 J/kg and 9196 J/kg, respectively. Bacillus subtilis showed limitation to digest feather in large scale, but the resistance of Bs to digestion was observed since it grown on feces culture.
Antimicrobial Resistance of Salmonella enteritidis and Salmonella typhimurium Isolated from Laying Hens, Table Eggs, and Humans with Respect to Antimicrobial Activity of Biosynthesized Silver Nanoparticles
Authors: Abou Elez Rasha M. M. , Elsohaby Ibrahim , El-Gazzar Nashwa , Tolba Hala M. N. , Abdelfatah Eman N. , Abdellatif Samah S. , Mesalam Ahmed Atef , Tahoun Asmaa B. M. B.
Published: December 2021

Salmonella enterica is one of the most common causes of foodborne illness worldwide. Contaminated poultry products, especially meat and eggs are the main sources of human salmonellosis. Thus, the aim of the present study was to determine prevalence, antimicrobial resistance profiles, virulence, and resistance genes of Salmonella Enteritidis (S. enteritidis) and Salmonella Typhimurium (S. Typhimurium) isolated from laying hens, table eggs, and humans, in Sharkia Governorate, Egypt. The antimicrobial activity of Biosynthesized Silver Nanoparticles (AgNPs) was also evaluated. Salmonella spp. were found in 19.3% of tested samples with laying hens having the highest isolation rate (33.1%). S. Enteritidis) (5.8%), and S. Typhimurium (2.8%) were the dominant serotypes. All isolates were ampicillin resistant (100%); however, none of the isolates were meropenem resistant. Multidrug-resistant (MDR) was detected in 83.8% of the isolates with a multiple antibiotic resistance index of 0.21 to 0.57. Most isolates (81.1%) had at least three virulence genes (sopB, stn, and hilA) and none of the isolates harbored the pefA gene; four resistance genes (blaTEM, tetA, nfsA, and nfsB) were detected in 56.8% of the examined isolates. The AgNPs biosynthesized by Aspergillus niveus exhibit an absorption peak at 420 nm with an average size of 27 nm. AgNPs had a minimum inhibitory concentration of 5 µg/mL against S. enteritidis and S. typhimurium isolates and a minimum bactericidal concentration of 6 and 8 µg/mL against S. enteritidis and S. typhimurium isolates, respectively. The bacterial growth and gene expression of S. enteritidis and S. typhimurium isolates treated with AgNPs were gradually decreased as storage time was increased. In conclusion, this study indicates that S. enteritidis and S. typhimurium isolated from laying hens, table eggs, and humans exhibits resistance to multiple antimicrobial classes. The biosynthesized AgNPs showed potential antimicrobial activity against MDR S. enteritidis and S. typhimurium isolates. However, studies to assess the antimicrobial effectiveness of the biosynthesized AgNPs in laying hen farms are warranted.
Common Infections and Antibiotic Prescribing during the First Year of the COVID-19 Pandemic: A Primary Care-Based Observational Cohort Study
Authors: Boeijen Josi A. , van der Velden Alike W. , Hullegie Saskia , Platteel Tamara N. , Zwart Dorien L. M. , Damoiseaux Roger A. M. J. , Venekamp Roderick P. , van de Pol Alma C.
Published: December 2021

Presentation and antibiotic prescribing for common infectious disease episodes decreased substantially during the first COVID-19 pandemic wave in Dutch general practice. We set out to determine the course of these variables during the first pandemic year. We conducted a retrospective observational cohort study using routine health care data from the Julius General Practitioners’ Network. All patients registered in the pre-pandemic year (n = 425,129) and/or during the first pandemic year (n = 432,122) were included. Relative risks for the number of infectious disease episodes (respiratory tract/ear, urinary tract, gastrointestinal, and skin), in total and those treated with antibiotics, and proportions of episodes treated with antibiotics (prescription rates) were calculated. Compared to the pre-pandemic year, primary care presentation for common infections remained lower during the full first pandemic year (RR, 0.77; CI, 0.76–0.78), mainly attributed to a sustained decline in respiratory tract/ear and gastrointestinal infection episodes. Presentation for urinary tract and skin infection episodes declined during the first wave, but returned to pre-pandemic levels during the second and start of the third wave. Antibiotic prescription rates were lower during the full first pandemic year (24%) as compared to the pre-pandemic year (28%), mainly attributed to a 10% lower prescription rate for respiratory tract/ear infections; the latter was not accompanied by an increase in complications. The decline in primary care presentation for common infections during the full first COVID-19 pandemic year, together with lower prescription rates for respiratory tract/ear infections, resulted in a substantial reduction in antibiotic prescribing in Dutch primary care.
Levofloxacin Versus Ciprofloxacin-Based Prophylaxis during the Pre-Engraftment Phase in Allogeneic Hematopoietic Stem Cell Transplant Pediatric Recipients: A Single-Center Retrospective Matched Analysis
Authors: Servidio Alessia G. , Simeone Roberto , Zanon Davide , Barbi Egidio , Maximova Natalia
Published: December 2021

Infectious complications are the most common and significant cause of mortality and morbidity after allogeneic hematopoietic stem cell transplantation (HSCT). Antibacterial prophylaxis in pediatric cancer patients is a controversial issue. Our study compared the outcomes of levofloxacin versus ciprofloxacin prophylaxis in allogeneic HSCT pediatric recipients treated for hematological malignancies. A total of 120 patients received levofloxacin prophylaxis, and 60 patients received ciprofloxacin prophylaxis. Baseline characteristics such as age, gender, primary diagnosis, type of conditioning, donor type, stem cell source, and supportive care of the patients were similar, and duration of antibiotics prophylaxis was similar. Both prophylaxis regimens demonstrated the same efficacy on the risk of febrile neutropenia and severe complications such as sepsis, the same rate of overall mortality, hospital readmission, and length of hospital stay. Levofloxacin prophylaxis was associated with significantly lower cumulative antibiotic exposure. The median of Gram-positive infection-related antibiotic days was 10 days in the levofloxacin group versus 25 days in the ciprofloxacin group (p < 0.0001). The median of Gram-negative infection-related antibiotics was 10 days in the levofloxacin group compared with 20 days in the ciprofloxacin group (p < 0.0001). The number of days with body temperature ≥38 °C was significantly less in the levofloxacin group (p < 0.001).
In Vivo Antibacterial Efficacy of Nanopatterns on Titanium Implant Surface: A Systematic Review of the Literature
Authors: Sun Yang , Yang Yang , Jiang Weibo , Bai Haotian , Liu He , Wang Jincheng
Published: December 2021

Background: Bionic surface nanopatterns of titanium (Ti) materials have excellent antibacterial effects in vitro for infection prevention. To date, there is a lack of knowledge about the in vivo bactericidal outcomes of the nanostructures on the Ti implant surfaces. Methods: A systematic review was performed using the PubMed, Embase, and Cochrane databases to better understand surface nanoscale patterns’ in vivo antibacterial efficacy. The inclusion criteria were preclinical studies (in vivo) reporting the antibacterial activity of nanopatterns on Ti implant surface. Ex vivo studies, studies not evaluating the antibacterial activity of nanopatterns or surfaces not modified with nanopatterns were excluded. Results: A total of five peer-reviewed articles met the inclusion criteria. The included studies suggest that the in vivo antibacterial efficacy of the nanopatterns on Ti implants’ surfaces seems poor. Conclusions: Given the small number of literature results, the variability in experimental designs, and the lack of reporting across studies, concluding the in vivo antibacterial effectiveness of nanopatterns on Ti substrates’ surfaces remains a big challenge. Surface coatings using metallic or antibiotic elements are still practical approaches for this purpose. High-quality preclinical data are still needed to investigate the in vivo antibacterial effects of the nanopatterns on the implant surface.
Using Lactobacilli to Fight Escherichia coli and Staphylococcus aureus Biofilms on Urinary Tract Devices
Authors: Carvalho Fábio M. , Mergulhão Filipe J. M. , Gomes Luciana C.
Published: December 2021

The low efficacy of conventional treatments and the interest in finding natural-based approaches to counteract biofilm development on urinary tract devices have promoted the research on probiotics. This work evaluated the ability of two probiotic strains, Lactobacillus plantarum and Lactobacillus rhamnosus, in displacing pre-formed biofilms of Escherichia coli and Staphylococcus aureus from medical-grade silicone. Single-species biofilms of 24 h were placed in contact with each probiotic suspension for 6 h and 24 h, and the reductions in biofilm cell culturability and total biomass were monitored by counting colony-forming units and crystal violet assay, respectively. Both probiotics significantly reduced the culturability of E. coli and S. aureus biofilms, mainly after 24 h of exposure, with reduction percentages of 70% and 77% for L. plantarum and 76% and 63% for L. rhamnosus, respectively. Additionally, the amount of E. coli biofilm determined by CV staining was maintained approximately constant after 6 h of probiotic contact and significantly reduced up to 67% after 24 h. For S. aureus, only L. rhamnosus caused a significant effect on biofilm amount after 6 h of treatment. Hence, this study demonstrated the potential of lactobacilli to control the development of pre-established uropathogenic biofilms.
Early Diagnosis and Antibiotic Treatment Combined with Multicomponent Hemodynamic Support for Addressing a Severe Case of Lemierre’s Syndrome
Authors: Recchia Andreaserena , Cascella Marco , Altamura Sabrina , Borrelli Felice , De Nittis Nazario , Dibenedetto Elisabetta , Labonia Maria , Pavone Giovanna , Del Gaudio Alfredo
Published: December 2021

A 20-year-old man was admitted to the intensive care unit for septic shock due to Lemierre’s syndrome. It is a rare syndrome that manifests as an upper respiratory infection, although systemic involvement, severe coagulopathy, and multi-organ failure can dangerously complicate the clinical picture. In this syndrome, sepsis-related neuroendocrine dysregulation and microcirculation impairment can have a rapid deleterious progression. Consequently, proper diagnosis, early source control, and appropriate antibiotics administration are mandatory to improve the prognosis. The intensive treatment is aimed at limiting organ damage through hemodynamic optimization. Remarkably, in septic shock due to Lemierre’s syndrome, hemodynamic optimization can be achieved through the synergic effect of norepinephrine, argipressin, and hydrocortisone.
Point Prevalence Survey of Antimicrobial Utilization in Ghana’s Premier Hospital: Implications for Antimicrobial Stewardship
Authors: Ankrah Daniel , Owusu Helena , Aggor Asiwome , Osei Anthony , Ampomah Agneta , Harrison Mark , Nelson Frempomaa , Aboagye Grace Owusu , Ekpale Priscilla , Laryea Jennifer , Selby Julia , Amoah Serwaa , Lartey Linda , Addison Okaikor , Bruce Elizabeth , Mahungu Joyce , Mirfenderesky Mariyam
Published: December 2021

The first comprehensive point prevalence survey at the Korle Bu Teaching Hospital (KBTH) was performed as part of the 2019 Global Point Prevalence Survey (Global-PPS) on antimicrobials. The aim was to establish a PPS baseline for the whole hospital and to identify required stewardship interventions. The PPS was conducted over three days in June 2019 using the GLOBAL-PPS standardized method for surveillance of antimicrobial utilization in hospitals to evaluate antimicrobial prescribing. In all, 988 patients were admitted to 69 wards. Overall antimicrobial prevalence was 53.3%. More community-acquired infections (CAI) were treated empirically compared to health-care associated infections (94.0% vs. 86.1% respectively, p = 0.002). Main indications for prescribing antimicrobials were pneumonia (18.4%), skin and soft tissue infections (11.4%) and sepsis (11.1%). Among antimicrobials, systemic antibiotics accounted for 83.5%, of which amoxicillin with beta-lactam inhibitor (17.5%), metronidazole (11.8%) and ceftriaxone (11.5%) dominated. Guideline compliance was 89.0%. Stop/review dates were completed in 33.4% and documented reason was recorded in 53.0% of all prescriptions. If the findings in this PPS can be addressed antimicrobial stewardship at the KBTH stands to improve significantly.
Impact of the COVID-19 Pandemic on Community Antibiotic Prescribing and Stewardship: A Qualitative Interview Study with General Practitioners in England
Authors: Borek Aleksandra J. , Maitland Katherine , McLeod Monsey , Campbell Anne , Hayhoe Benedict , Butler Christopher C. , Morrell Liz , Roope Laurence S. J. , Holmes Alison , Walker Ann Sarah , Tonkin-Crine Sarah ,   on behalf of the STEP-UP Study Team
Published: December 2021

The COVID-19 pandemic has had a profound impact on the delivery of primary care services. We aimed to identify general practitioners’ (GPs’) perceptions and experiences of how the COVID-19 pandemic influenced antibiotic prescribing and antimicrobial stewardship (AMS) in general practice in England. Twenty-four semi-structured interviews were conducted with 18 GPs at two time-points: autumn 2020 (14 interviews) and spring 2021 (10 interviews). Interviews were audio-recorded, transcribed and analysed thematically, taking a longitudinal approach. Participants reported a lower threshold for antibiotic prescribing (and fewer consultations) for respiratory infections and COVID-19 symptoms early in the pandemic, then returning to more usual (pre-pandemic) prescribing. They perceived the pandemic as having had less impact on antibiotic prescribing for urinary and skin infections. Participants perceived the changing ways of working and consulting (e.g., proportions of remote and in-person consultations) in addition to changing patient presentations and GP workloads as influencing the fluctuations in antibiotic prescribing. This was compounded by decreased engagement with, and priority of, AMS due to COVID-19-related urgent priorities. Re-engagement with AMS is needed, e.g., through reviving antibiotic prescribing feedback and targets/incentives. The pandemic disrupted, and required adaptations in, the usual ways of working and AMS. It is now important to identify opportunities, e.g., for re-organising ways of managing infections and AMS in the future.
Genomic Insights into the Distribution and Phylogeny of Glycopeptide Resistance Determinants within the Actinobacteria Phylum
Authors: Andreo-Vidal Andrés , Binda Elisa , Fedorenko Victor , Marinelli Flavia , Yushchuk Oleksandr
Published: December 2021

The spread of antimicrobial resistance (AMR) creates a challenge for global health security, rendering many previously successful classes of antibiotics useless. Unfortunately, this also includes glycopeptide antibiotics (GPAs), such as vancomycin and teicoplanin, which are currently being considered last-resort drugs. Emerging resistance towards GPAs risks limiting the clinical use of this class of antibiotics—our ultimate line of defense against multidrug-resistant (MDR) Gram-positive pathogens. But where does this resistance come from? It is widely recognized that the GPA resistance determinants—van genes—might have originated from GPA producers, such as soil-dwelling Gram-positive actinobacteria, that use them for self-protection. In the current work, we present a comprehensive bioinformatics study on the distribution and phylogeny of GPA resistance determinants within the Actinobacteria phylum. Interestingly, van-like genes (vlgs) were found distributed in different arrangements not only among GPA-producing actinobacteria but also in the non-producers: more than 10% of the screened actinobacterial genomes contained one or multiple vlgs, while less than 1% encoded for a biosynthetic gene cluster (BGC). By phylogenetic reconstructions, our results highlight the co-evolution of the different vlgs, indicating that the most diffused are the ones coding for putative VanY carboxypeptidases, which can be found alone in the genomes or associated with a vanS/R regulatory pair.
Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic
Authors: Ferraboschi Patrizia , Ciceri Samuele , Grisenti Paride
Published: December 2021

Lysozyme is a ~14 kDa protein present in many mucosal secretions (tears, saliva, and mucus) and tissues of animals and plants, and plays an important role in the innate immunity, providing protection against bacteria, viruses, and fungi. Three main different types of lysozymes are known: the c-type (chicken or conventional type), the g-type (goose type), and the i-type (invertebrate type). It has long been the subject of several applications due to its antimicrobial properties. The problem of antibiotic resistance has stimulated the search for new molecules or new applications of known compounds. The use of lysozyme as an alternative antibiotic is the subject of this review, which covers the results published over the past two decades. This review is focused on the applications of lysozyme in medicine, (the treatment of infectious diseases, wound healing, and anti-biofilm), veterinary, feed, food preservation, and crop protection. It is available from a wide range of sources, in addition to the well-known chicken egg white, and its synergism with other compounds, endowed with antimicrobial activity, are also summarized. An overview of the modified lysozyme applications is provided in the form of tables.
Dietary Vitamin C and Vitamin C Derived from Vegetables Are Inversely Associated with the Risk of Depressive Symptoms among the General Population
Authors: Wang Anni , Luo Jia , Zhang Tianhao , Zhang Dongfeng
Published: December 2021

Vitamin C is a water-soluble antioxidant. Reducing the level of oxidative stress can alleviate depression. Therefore, we investigated the correlation between dietary vitamin C intake and the risk of depressive symptoms in the general population. Data from the 2007–2018 National Health and Nutrition Examination Survey were used in our study. The dietary intake of vitamin C was assessed by two 24-h dietary recalls. Depressive symptoms were assessed with the Patient Health Questionnaire-9. Logistic regression and restricted cubic spline models were applied to assess the relationship between dietary vitamin C intake and the risk of depressive symptoms. The multivariate adjusted odds ratio (95% confidence interval) of depressive symptoms for the highest vs. lowest category of dietary vitamin C intake and vitamin C intake derived from vegetables were 0.73 (0.58–0.91) and 0.73 (0.56–0.95). In subgroup analyses, dietary vitamin C intake was negatively correlated with the risk of depressive symptoms in females 18–39 years old and 40–59 year-old groups. A dose-response analysis showed that there was a nonlinear relationship between dietary vitamin C intake and the risk of depressive symptoms. Dietary vitamin C intake and vitamin C intake derived from vegetables were inversely associated with the risk of depressive symptoms among the general population. We recommend increasing the intake of vegetables in daily diet.
Cyanidin-3-O-glucoside Regulates the Expression of Ucp1 in Brown Adipose Tissue by Activating Prdm16 Gene
Authors: Han Suping , Yang Yafan , Lu Yanan , Guo Jielong , Han Xue , Gao Yunxiao , Huang Weidong , You Yilin , Zhan Jicheng
Published: December 2021

(1) Background: Brown adipose tissue (BAT) burns energy to produce heat. Cyanidin-3-O-glucoside (C3G) can then enhance the thermogenic ability of BAT in vivo. However, the mechanism by which C3G regulates Ucp1 protein expression remains unclear. (2) Methods: In this study, C3H10T12 brown adipose cells and db/db mice and mice with high-fat, high-fructose, diet-induced obesity were used as the model to explore the effect of C3G on the expression of the Ucp1 gene. Furthermore, the 293T cell line was used for an in vitro cell transgene, a double luciferase reporting system, and yeast single hybridization to explore the mechanism of C3G in regulating Ucp1 protein. (3) Results: we identified that, under the influence of C3G, Prdm16 directly binds to the −500 to −150 bp promoter region of Ucp1 to activate its transcription and, thus, facilitate BAT programming. (4) Conclusions: This study clarified the mechanism by which C3G regulates the expression of the Ucp1 gene of brown fat to a certain extent.
Catalase Overexpression Drives an Aggressive Phenotype in Glioblastoma
Authors: Flor Susanne , Oliva Claudia R. , Ali Md Yousuf , Coleman Kristen L. , Greenlee Jeremy D. , Jones Karra A. , Monga Varun , Griguer Corinne E.
Published: December 2021

Glioblastoma remains the deadliest form of brain cancer, largely because these tumors become resistant to standard of care treatment with radiation and chemotherapy. Intracellular production of reactive oxygen species (ROS) is necessary for chemo- and radiotherapy-induced cytotoxicity. Here, we assessed whether antioxidant catalase (CAT) affects glioma cell sensitivity to temozolomide and radiation. Using The Cancer Genome Atlas database, we found that CAT mRNA expression is upregulated in glioma tumor tissue compared with non-tumor tissue, and the level of expression negatively correlates with the overall survival of patients with high-grade glioma. In U251 glioma cells, CAT overexpression substantially decreased the basal level of hydrogen peroxide, enhanced anchorage-independent cell growth, and facilitated resistance to the chemotherapeutic drug temozolomide and ionizing radiation. Importantly, pharmacological inhibition of CAT activity reduced the proliferation of glioma cells isolated from patient biopsy samples. Moreover, U251 cells overexpressing CAT formed neurospheres in neurobasal medium, whereas control cells did not, suggesting that the radio- and chemoresistance conferred by CAT may be due in part to the enrichment of glioma stem cell populations. Finally, CAT overexpression significantly decreased survival in an orthotopic mouse model of glioma. These results demonstrate that CAT regulates chemo- and radioresistance in human glioma.
Assessment of the Impacts of Climate Change on Power Systems: The Italian Case Study
Authors: Tina Giuseppe Marco , Nicolosi Claudio F.
Published: December 2021

Climate change due to the greenhouse effect will affect meteorological variables, which in turn will affect the demand for electrical energy and its generation in coming years. These impacts will become increasingly important in accordance with the increasing penetration of renewable, non-programmable energy sources (e.g., wind and solar). Specifically, the speed and amplitude of power system transformation will be different from one country to another according to many endogenous and exogenous factors. Based on a literature review, this paper focuses on the impact of climate change on the current, and future, Italian power system. The paper shows a wide range of results, due not just to the adopted climate change models used, but also to the models used to assess the impact of meteorological variables on electricity generation and demand. Analyzing and interpreting the reasons for such differences in the model results is crucial to perform more detailed numerical analyses on the adequacy and reliability of power systems. Concerning Italian future scenarios, the double impact of uncertainties in national policies and changes in power plant productivity and demand, has been considered and addressed.
Production of Synthetic Models for Neuro-Oncology Training by Additive Manufacturing
Authors: Saceleanu Vicentiu , Paz Rubén , García Joshua , Rivero Yamilet , Cîndea Cosmin-Nicodim , Cacciotti Ilaria , Monzón Mario
Published: December 2021

Neurosurgery is one of the medical specialties in which the practical training of students is more limiting since it requires a high degree of preparation for the interventions to be satisfactory. That is why the manufacture of synthetic models through additive manufacturing (AM) arises to develop the skills that the neurosurgeon requires. The present work is aimed at validating the use of AM for the neurosurgery training. To this regard, a meningioma case study was considered, and suitable materials and more appropriate AM technology were identified for a low-cost production of synthetic models of both skulls and brains with tumors. The skull was manufactured by material extrusion AM with two materials, a commercial composite filament composed of polylactic acid (PLA) with calcium carbonate (used in the area to be treated during the cutting process, due to its mechanical properties more comparable to those of the native bone, with 30% infill density) and standard PLA without additives (used in the rest of the model, with 20% infill density). On the other hand, different casting silicones in different proportions were tested under compression molding to find the best combination to mimic the brain and tumor. Ten synthetic models of a real-case meningioma were manufactured and used as training material by students in the neurosurgery sector, who rated the proposed training approach very highly, considering the employment of printed models as a key resource for improving their surgical skills.
Removal of Inflammatory Tissue/Product by Sinus Membrane Puncturing during Lateral Sinus Augmentation in Asymptomatic Patients with Severely Opacified Sinuses: A Case Series
Authors: Park Won-Bae , Park Jung Soo , Han Ji-Young , Shin Seung-Il , Lim Hyun-Chang
Published: December 2021

It is generally recommended that severe sinus membrane (SM) thickening should be treated prior to maxillary sinus augmentation (MSA), but during lateral MSA, inflammatory tissue/product may be removed by puncturing the SM. The present case report demonstrates surgical experience of lateral MSA with simultaneous inflammatory tissue/product removal for sinuses with severe opacification. In three patients requiring dental implant placement in the posterior maxilla, severe SM thickening was observed, but they were asymptomatic. The SM was gently elevated, followed by puncturing the SM, removing inflammatory tissue via the punctured site, draining, and thorough saline irrigation. Then, bone grafting and implant placement were performed with extra care not to spread bone substitute material into the punctured area. The postoperative pain following this procedure was more severe as compared to conventional MSA. Nasal bleeding was reported for 2–3 days. All implants were successfully integrated and demonstrated adequate function. Tissue samples retrieved during the surgery showed advanced inflammatory cell infiltration. The follow-up cone-beam computed tomographic scans revealed a significant reduction in SM thickening. In conclusion, inflammatory tissue/product removal by puncturing the SM can be applied during lateral MSA. However, more data should be needed due to the empirical nature of the present outcomes.
Influence of Contamination of Gear Oils in Relation to Time of Operation on Their Lubricity
Authors: Gil Leszek , Przystupa Krzysztof , Pieniak Daniel , Kozłowski Edward , Antosz Katarzyna , Gauda Konrad , Izdebski Paweł
Published: December 2021

The quality and reliability of consumables, including gear oils, results in the failure-free operation of the transmission components in heavy trucks. It is known that oil viscosity is essential for all lubricated tribopairs for wear and friction reduction in all vehicles with a gearbox. Viscosity may be influenced by the contamination that wear products can impart on the oil. Oil contamination can also affect lubrication efficiency in the boundary friction conditions in gearboxes where slips occur (including bevel and hypoid gearboxes). The present research focused on this issue. An obvious hypothesis was adopted, where it was theorized that exploiting the contaminants that are present in gear oil may affect how the lubricating properties of gear oils deteriorate. Laboratory tests were performed on contaminants that are commonly found in gear oil using the Parker Laser CM20. The study was designed to identify a number of different solid particles that are present in oil. At the second stage, friction tests were conducted for a friction couple “ball-on-disc” in an oil bath at 90 °C on a CSM microtribometer. The quantitative contamination of the gear oils that contained solid particles and the curves representing the friction coefficients of fresh oils with a history of exploitation were compared. The test results were statistically analysed. Exploitation was shown to have a significant impact on the contamination of gear oils. It was revealed that the contamination and the mileage had no effect on the tested oils.
Research on Shear Behavior of Sand–Structure Interface Based on Monotonic and Cyclic Tests
Authors: Zhang Pei , Ding Shijia , Fei Kang
Published: December 2021

In order to study the shear behavior of the interface between sand and structure, a series of shear tests were carried out using an HJ-1 ring shear apparatus (Nanjing, China). First, through the monotonic shear tests, the loose sand and dense sand were sheared at the steel interface with different roughnesses. The results showed that when the interface was relatively smooth, the shear stress–shear displacement curves of loose sand and dense sand both exhibit strain hardening characteristics. When the interface was rough, the dense sand showed strain softening. The initial shear stiffness of the sand–steel interface increased with the increase in normal stress, interface roughness, or sand relative density. Then, considering the influence of initial shear stress, through the cyclic shear test, this work analyzed the shape of the loading and unloading curves and the development law of cumulative normal deformation, and discussed the change of loading and unloading shear stiffness under different stress level amplitudes and the residual deformation generated during the cycle. The research results showed that loose sand and dense sand generally shrunk in volume during the cycle. The initial loading process was similar to the case of static loading. In the later dynamic loading process, the shear shrinkage per cycle was relatively small and continued to develop. Additionally, it was found that the unloading stiffness of the sand–steel interface is always greater than the initial loading stiffness. As the number of cycles increases, the loading stiffness increases, and it may eventually approach the unloading stiffness.
Few-Shot Wideband Tympanometry Classification in Otosclerosis via Domain Adaptation with Gaussian Processes
Authors: Nie Leixin , Li Chao , Bozorg Grayeli Alexis , Marzani Franck
Published: December 2021

Otosclerosis is a common middle ear disease that requires a combination of examinations for its diagnosis in routine. In a previous study, we showed that this disease could be potentially diagnosed by wideband tympanometry (WBT) coupled with a convolutional neural network (CNN) in a rapid and non-invasive manner. We showed that deep transfer learning with data augmentation could be applied successfully on such a task. However, the involved synthetic and realistic data have a significant discrepancy that impedes the performance of transfer learning. To address this issue, a Gaussian processes-guided domain adaptation (GPGDA) algorithm was developed. It leveraged both the loss about the distribution distance calculated by the Gaussian processes and the loss of conventional cross entropy during the transferring. On a WBT dataset including 80 otosclerosis and 55 control samples, it achieved an area-under-the-curve of 97.9±1.1 percent after receiver operating characteristic analysis and an F1-score of 95.7±0.9 percent that were superior to the baseline methods (r=10, p<0.05, ANOVA). To understand the algorithm’s behavior, the role of each component in the GPGDA was experimentally explored on the dataset. In conclusion, our GPGDA algorithm appears to be an effective tool to enhance CNN-based WBT classification in otosclerosis using just a limited number of realistic data samples.
Sequential Movie Genre Prediction Using Average Transition Probability with Clustering
Authors: Kim Jihyeon , Kim Jinkyung , Choi Jaeyoung
Published: December 2021

In recent movie recommendations, one of the most important issues is to predict the user’s sequential behavior to be able to suggest the next movie to watch. However, capturing such sequential behavior is not easy because each user’s short-term or long-term behavior must be taken into account. For this reason, many research results show that the performance of recommending a specific movie is not good in a sequential recommendation. In this paper, we propose a cluster-based method for classifying users with similar movie purchase patterns and a movie genre prediction algorithm rather than the movie itself considering their short-term and long-term behaviors. The movie genre prediction does not recommend a specific movie, but it predicts the genre for the next movie to watch in consideration of each user’s preference for the movie genre based on the genre included in the movie. Using this, it will be possible to provide appropriate guidelines for recommending movies including the genres to users who tend to prefer a specific genre. In particular, in this study, users with similar genre preferences are organized into clusters to recommend genres. For clusters that do not have relatively specific tendencies, genre prediction is performed by appropriately trimming genres that are not necessary for recommendation in order to improve performance. We evaluate our method on well-known movie data sets and qualitatively determine that it captures personalized dynamics and is able to make meaningful recommendations.
Efficient Key-Value Data Placement for ZNS SSD
Authors: Oh Gijun , Yang Junseok , Ahn Sungyong
Published: December 2021

Log-structured merge-tree (LSM-Tree)-based key–value stores are attracting attention for their high I/O (Input/Output) performance due to their sequential write characteristics. However, excessive writes caused by compaction shorten the lifespan of the Solid-state Drive (SSD). Therefore, there are several studies aimed at reducing garbage collection overhead by using Zoned Namespace ZNS; SSD in which the host can determine data placement. However, the existing studies have limitations in terms of performance improvement because the lifetime and hotness of key–value data are not considered. Therefore, in this paper, we propose a technique to minimize the space efficiency and garbage collection overhead of SSDs by arranging them according to the characteristics of key–value data. The proposed method was implemented by modifying ZenFS of RocksDB and, according to the result of the performance evaluation, the space efficiency could be improved by up to 75%.
Accurate Estimation of Bicarbonate and Acetic Acid Concentrations with Wider Ranges in Anaerobic Media Using Classical FOS/TAC Titration Method
Authors: Liu Xiaojun , André Laura , Mercier-Huat Maël , Grosmaître Jean-Marie , Pauss André , Ribeiro Thierry
Published: December 2021

The determination of a volatile fatty acid content (FOS) and total alkalinity (TAC) can be carried out using Nordmann’s FOS/TAC titration method developed in the 1970s. This two-point titration (pH = 5 and 4.4) can be simply implemented and is widely employed by both the academic and industrial worlds. However, the present study proves that Nordmann’s method is only valid in limited ranges, since the titration of one FOS and TAC has an impact on the determination of the other, especially in extreme conditions. The present work develops a numerical tool with Scilab simulating the acid–base equilibria of titration. The program is efficient in predicting the experimental equivalent volumes obtained from Nordmann’s method with different combinations of sodium acetate and sodium bicarbonate contents. The mean absolute percentage errors (MAPE) between the simulation and experiment are below 7%. Two new formulas are developed, considering both equivalent volumes at pH = 5 and 4.4 to calibrate FOS and TAC values. The proposed formulas show their good performance in predicting various combinations of FOS and TAC contents in an anaerobic digestate at TAC ranging from 0 to 20,000 mg CaCO3·L−1 and FOS ranging from 0 to 31,000 mg HAc·L−1.
The Calculated Circadian Effects of Light Exposure from Commuting
Authors: Lu Yihan , Hu Wenye , Davis Wendy
Published: December 2021

Light entrains human circadian rhythms, but increased time spent indoors and decreased daylight exposure may disrupt human circadian regulation and cause health problems. Much research is focused on improving indoor lighting conditions to minimize the adverse circadian impact of electric lights, and few studies investigate the circadian impact of daylight during the incidental time that people spend outdoors. For instance, when people commute from home to work, they are exposed to daylight. The purpose of this study is to investigate daylight’s impact on commuters’ circadian rhythms. Measurements of the illuminance and the spectral irradiance distribution (SID) of daylight were taken for three modes of commuting: driving, riding on trains, and walking; and under different weather conditions, on different days, and at different locations throughout the summer and autumn in the Sydney metropolitan region in Australia. With the SID data, three metrics were calculated to estimate the circadian impacts: α-opic irradiance, circadian stimulus (CS), and equivalent melanopic lux (EML). The results suggest that driving or walking on sunny or cloudy days and riding trains on sunny days are beneficial for the commuters’ circadian synchronization.
Rapid Estimation of Earthquake Magnitude and Source Parameters Using Genetic Algorithms
Authors: Novianty Astri , Meilano Irwan , Machbub Carmadi , Widiyantoro Sri , Susilo Susilo
Published: December 2021

To minimize the impacts of large losses and optimize the emergency response when a large earthquake occurs, an accurate early warning of an earthquake or tsunami is crucial. One important parameter that can provide an accurate early warning is the earthquake’s magnitude. This study proposes a method for estimating the magnitude, and some of the source parameters, of an earthquake using genetic algorithms (GAs). In this study, GAs were used to perform an inversion of Okada’s model from earthquake displacement data. In the first stage of the experiment, the GA was used to inverse the displacement calculated from the forward calculation in Okada’s model. The best performance of the GA was obtained by tuning the hyperparameters to obtain the most functional configuration. In the second stage, the inversion method was tested on GPS time series data from the 2011 Tohoku Oki earthquake. The earthquake’s displacement was first estimated from GPS time series data using a detection and estimation formula from previous research to calculate the permanent displacement value. The proposed method can estimate an earthquake’s magnitude and four source parameters (i.e., length, width, rake, and slip) close to the real values with reasonable accuracy.
The Application of Microplasma in the Terahertz Field: A Review
Authors: Guo Yue , Wu Shuqun , Liu Xuhui , Yang Lu , Zhang Chaohai
Published: December 2021

Terahertz functional devices are essential to the advanced applications of terahertz radiation in biology and medicine, nanomaterials, and wireless communications. Due to the small size and high plasma frequency of microplasma, the interaction between terahertz radiation and microplasma provides opportunities for developing functional terahertz devices based on microplasma. This paper reviews the applications of microplasma in terahertz sources, terahertz amplifiers, terahertz filters, and terahertz detectors. The prospects and challenges of the interdisciplinary research between microplasma and terahertz technology are discussed.