bluuebunny/crossref_metadata_2025 huggingface dataset:
Re-examining poikilocytosis in goats: prevalence, type and association with age and disease
Authors: Vasilatis Demitria M. , Christopher Mary M.
Published: August 2023

Background

Domestic goats (Capra aegagrus hircus) are a food, fiber and companion animal. Abnormal erythrocyte shapes (poikilocytes) are considered normal in young goats, but their association with disease is not well described. Likewise, there is little information on the significance of poikilocytosis in adult goats.

Objective

The objective of this study was to investigate the prevalence, severity and type of poikilocytosis in young and adult goats and its association with age, sex, breed, laboratory results, and underlying disease.

Methods

We retrospectively examined clinical and laboratory data from 1254 goats presented at the University of California-Davis Veterinary Medical Teaching Hospital from 1997 to 2019. We analyzed 313 blood smears from goats with moderate or marked (MOD-MKD) poikilocytosis on initial blood smear evaluation. Number and type of poikilocytes per 1000 red blood cells (RBCs) were enumerated. Laboratory values and primary disease categories were compared with the severity and type of poikilocytosis.

Results

Kids (<6 mos) and juveniles (>6 mos to <1 year) had a higher prevalence of MOD-MKD poikilocytosis (95/210, 45.2% kids; 27/59, 45.8% juveniles) than adult goats (≥1 year; 190/982, 19.3%) (p < 0.001). Kids had a higher percentage of elliptocytes, dacryocytes, and schistocytes and a lower percentage of polygonal and spiculated RBCs than juvenile and adult goats (p < 0.001). Of goats with MOD-MKD (vs NONE-SLIGHT) poikilocytosis, kids had lower HGB and MCH, and higher RDW (p ≤ 0.02); juveniles and adult goats had lower HCT, MCV, MCH, and albumin concentration (p ≤ 0.01), and all age groups had lower total CO2 concentration and higher PLT counts (p < 0.03). Adult goats with MOD-MKD poikilocytosis also had higher BUN:Cr ratios (p = 0.005). Gastrointestinal parasitism, Johne’s disease, diarrhea/enteritis, lice, hepatic disease and renal disease (but not urolithiasis) were significantly associated with MOD-MKD poikilocytosis (p < 0.001). Goats with hepatic and renal disease had a higher prevalence and percentage of spiculated cells (p = 0.001). Goats with Johne’s disease had a higher prevalence of polygonal cells (93.3%) and dacryocytes (66.7%) than other diseases, and elliptocytes predominated in a higher proportion (36.0%) of adult goats with GI parasitism vs other diseases (p < 0.05).

Conclusion

These findings suggest that iron deficiency is an important pathophysiologic mechanism of poikilocytosis in juvenile and adult goats, and possibly in kids, whether due to iron-restricted erythropoiesis, chronic hemorrhage, functional iron deficiency, or a combination of these mechanisms. Further investigation into the detection and monitoring of iron deficiency and the value of poikilocytosis as a diagnostic marker of iron status in goats is warranted.
Excess Deaths of Gastrointestinal, Liver, and Pancreatic Diseases During the COVID-19 Pandemic in the United States
Authors: Han Lefei , Shi Haoting , Li Yongxuan , Qi Hongchao , Wang Yuhua , Gu Jiawei , Wu Jiayin , Zhao Shi , Cao Peihua , Xu Lan , Deng Xiaobei , Yao Xiaoxin I. , Ran Jinjun
Published: August 2023

Objectives: To evaluate excess deaths of gastrointestinal, liver, and pancreatic diseases in the United States during the COVID-19 pandemic.

Methods: We retrieved weekly death counts from National Vital Statistics System and fitted them with a quasi-Poisson regression model. Cause-specific excess deaths were calculated by the difference between observed and expected deaths with adjustment for temporal trend and seasonality. Demographic disparities and temporal-spatial patterns were evaluated for different diseases.

Results: From March 2020 to September 2022, the increased mortality (measured by excess risks) for Clostridium difficile colitis, gastrointestinal hemorrhage, and acute pancreatitis were 35.9%; 24.8%; and 20.6% higher than the expected. For alcoholic liver disease, fibrosis/cirrhosis, and hepatic failure, the excess risks were 1.4–2.8 times higher among younger inhabitants than older inhabitants. The excess deaths of selected diseases were persistently observed across multiple epidemic waves with fluctuating trends for gastrointestinal hemorrhage and fibrosis/cirrhosis and an increasing trend for C. difficile colitis.

Conclusion: The persistently observed excess deaths of digestive diseases highlights the importance for healthcare authorities to develop sustainable strategies in response to the long-term circulating of SARS-CoV-2 in the community.
The Potential of Hydroponic Seed Minituber Enrichment with the Endophyte Bacillus subtilis for Improving the Yield Components and Quality of Potato (Solanum tuberosum L.)
Authors: Pusenkova Liudmila , Lastochkina Oksana , Ercişli Sezai
Published: August 2023

This study examined the potential of using the endophytic bacteria Bacillus subtilis (10-4 and 26D) to enrich hydroponically grown potato seed minitubers (Solanum tuberosum L. cv. Bashkirsky) to improve plant growth, photosynthetic pigments, yield, and quality parameters, including nutritional value (i.e., macro-/microelements, vitamin C, anthocyanins). Potato seed minitubers, obtained from in-vitro-grown microplants in a hydroponic system, were inoculated with endophytic B. subtilis and subsequently grown in pots under controlled conditions. The results demonstrated the successful colonization of seed minitubers by B. subtilis, with subsequent distribution into growing plants (roots, shoots). The endophytes accelerated the plant’s phenological shifts, resulting in earlier emergence of sprouts, budding, and flowering compared with control plants. They also had increased leaf photosynthetic pigments (chlorophyll (Chl) a, Chl b, and carotenoids), total leaf area, and positively influenced leaf proline contents. The height of plants and number of stems per plant did not change significantly upon endophyte treatment, but improved root growth was observed throughout the experiment. As a result of endophyte application, there was an increase in stolon weight, number and size of tubers, and overall tuber yield. There were no significant differences in terms of total dry matter and starch content of the tubers compared to the control group, but the sugar levels decreased and the size of the starch grains was larger in endophyte-treated tubers. Furthermore, endophyte treatment resulted in an increased accumulation of nutrients including N, P, K, Cu, and Fe, as well as vitamin C and anthocyanins in harvested tubers. These findings indicate that colonization of hydroponically grown potato seed minitubers with endophytic B. subtilis (10-4 and 26D) before planting has great potential as an eco-friendly approach to obtain higher-quality seeds and to increase tuber yield and nutritional value in field conditions.
Portuguese Castanea sativa Genetic Resources: Characterization, Productive Challenges and Breeding Efforts
Authors: Braga Megan , Araújo Susana de Sousa , Sales Hélia , Pontes Rita , Nunes João
Published: August 2023

Chestnuts are multipurpose trees that grow mainly in the Northern Hemisphere due to their aptitude for fruit and wood production. These trees are vastly significant for the economy and wildlife. The widespread distribution of these trees demonstrates their genetic adaptability to many environmental conditions. The main varieties of European chestnut (Castanea sativa Miller) cultivated in Portugal, their productive challenges and breeding and biotechnological efforts developed over the last decades are described. This paper highlights the efforts focused on the improvement of varieties and rootstocks through selection and hybridization of European chestnut with the Asian species Castanea crenata Siebold and Zuccarini and Castanea mollissima Blume, which are resistant to ink disease, which have been the foundation of the Portuguese chestnut breeding programs. Breeding and biotechnological efforts developed over the last decades, focused on ink disease and chestnut blight resistance, are described. The potentialities of this research to stimulate the competitiveness of bioeconomy-based knowledge and innovation in the productive chestnut sector is also discussed.
Effects of Fertilization Mode on the Growth of Lactuca sativa L. and Soil Nutrients in Facilitated Cultivation
Authors: Li Wei , Wei Yanpeng , Zhao Jiale , Han Weiye , Li Ding , Wang Jianzhong , Zhao Mengfei , Chen Lin , Chen Limei , Zhou Lina
Published: August 2023

This paper aims to find a fertilization method that better matches the growth characteristics of vegetables and reduces the soil and environmental problems caused by unreasonable fertilization methods, in view of the strong buffering of the clayey black soil in the north and the mismatch between the amount of chemical fertilizer applied and the growth characteristics of vegetables during cultivation. In this experiment, Lactuca sativa L. (Lactuca sativa) was studied in a randomized complete block design in greenhouse and five different fertilizer application methods were arranged: exponential fertilization (EF), linear fertilization (LF), average fertilization (AF), one-time fertilization (OF), and no fertilization as a control group (CG). The effects of different fertilization methods on soil nutrients, agronomic traits of Lactuca sativa growth, and related quality were investigated, and the pattern of effects of different fertilization methods on growth and soil nutrients was obtained. The results of the experiment showed that the exponential fertilization method gradually took advantage as the growth time extended. The plant height (PLH), leaf length (LL), leaf width (LW), yield, soluble protein (SP), soluble sugar (SS), vitamin C (VC), and elemental nitrogen (EN) and potassium (EK) of Lactuca sativa were significantly improved under the exponential fertilization method compared with other fertilization methods, by 29.9 cm, 51.5 cm, 5.96 cm, 22, 2.32 kg/m2, 0.23%, 0.44%, 3.93%, 94.66 mg/kg, 1.58 g/kg, and 1.94 g/kg, respectively. The alkali-hydrolyzed nitrogen (SAN), available phosphorus (SAP), and available potassium (SAK) in the soil after fertilization were 139.69 mg/kg, 50.23 mg/kg, and 180.30 mg/kg, respectively. The above results showed that the exponential fertilization method not only improved the quality of Lactuca sativa and thus the quality of the crop growth traits, but also changed the soil nutrients favorably after fertilization, which is of some importance for the protection of black soils.
Transcriptome Analysis of Nitrogen-Deficiency-Responsive Genes in Two Potato Cultivars
Authors: Wei Qiaorong , Yin Yanbin , Deng Bin , Song Xuewei , Gong Zhenping , Shi Ying
Published: August 2023

The potato is the third largest food crop, and nitrogen fertilizer is important for increasing potato yields; however, the shallow root system of potatoes causes the nitrogen fertilizer utilization rate to be low, which results in waste and environmental pollution, meaning that high nitrogen efficiency breeding is highly significant for potatoes. In the high nitrogen efficiency breeding of potatoes, genes with a nitrogen-deficient response should first be identified, and RNA-seq is an efficient method for identifying nitrogen-deficiency-response genes. In this study, two potato cultivars, Dongnong 322 (DN322) and Dongnong 314 (DN314), were utilized, and two nitrogen fertilizer application rates (N0 and N1) were set for both cultivars. Through the determination of physiological indicators, we identified that DN314 is more sensitive to nitrogen fertilizer, while DN322 is relatively insensitive to nitrogen fertilizer. Samples were taken at the seedling and tuber formation stage. At the seedling stage, DN322 and DN314 had 573 and 150 differentially expressed genes (DEGs), while at the tuber formation stage, they had 59 and 1905 DEGs, respectively. A total of three genes related to a low-nitrogen response were obtained via the combined analysis of differentially expressed genes (DEGs) and weighted correlation network analysis (WGCNA), of which two genes were obtained at the tuber formation stage and one gene in the seedling stage, providing theoretical guidance for the high nitrogen efficiency breeding of potatoes.
Analyzing the Impact of Intensive Agriculture on Soil Quality: A Systematic Review and Global Meta-Analysis of Quality Indexes
Authors: Bedolla-Rivera Héctor Iván , Negrete-Rodríguez María de la Luz Xochilt , Gámez-Vázquez Francisco Paúl , Álvarez-Bernal Dioselina , Conde-Barajas Eloy
Published: August 2023

As the global population grows, the demand for food increases and puts a strain on food production systems and agricultural productivity, causing soil degradation. Soil quality indexes (SQIs) have been developed to maintain and improve soil quality. However, due to the variety of soils and SQIs, analyzing and comparing results has historically been difficult. Therefore, in this study, we carried out a systematic review with meta-analysis focused on soil quality studies of agricultural soils under intensive agriculture using the unified weighted additive SQI methodology (SQIU). Sixty-five observations of agricultural soil quality were analyzed. Chemical indicators were the most prevalent in the SQIUs, followed by physical and biological indicators. Conventional soil management had negative effects on soil quality (–7.55%). From the factors analyzed, the minimum database had a significant effect on the soil quality results, but not the number of indicators that made up the SQIU. The SQIU made up of chemical–biological indicators (CB) presented negatively overestimated measurements of soil quality (–32.53%), exaggerating the degradation of the analyzed soils. The indicators that correlated most strongly with the size of the effect on agricultural soil quality were the cation exchange capacity (CEC) and microbial biomass carbon (MBC). The SQIU is a feasible tool to interpret the quality of agricultural soils around the world, as it makes it possible to obtain a simple and generalized view of soil conditions.
Phosphorus Mobility in Heavily Manured and Waterlogged Soil Cultivated with Ryegrass (Lolium multiflorum)
Authors: Rupngam Thidarat , Messiga Aimé J. , Karam Antoine
Published: August 2023

Extended waterlogging (WL) conditions in heavily manured soils can change soil phosphorus (P) dynamics. We assessed the effects of soil moisture regimes (field capacity (FC) and WL) and P rates on (i) dry matter (DM) yield and P offtake of ryegrass, (ii) changes in soil Fe3+, Fe2+, and soil P, and (iii) risk of P leaching. The treatments were tested in a four-month greenhouse experiment using intact soil columns and annual ryegrass (Lolium multiflorum). The DM yield and P offtake were lower under WL compared with FC. The concentration of Fe3+ was 1984 mg kg−1 (0–30 cm) under FC, but 1213 mg kg−1 at 0–5 cm and 2024 mg kg−1 at 25–30 cm depth under WL. The concentration of Fe2+ was 244 mg kg−1 (0–30 cm) under FC, but 2897 at 0–5 cm and 687 mg kg−1 at 25–30 cm under WL. The water extractable P (Pw) was 12.7 mg kg−1 at 0–5 cm and 9.5 mg kg−1 at 25–30 cm under FC, but 8.6 mg kg−1 at 0–5 cm and 10.5 mg kg−1 at 25–30 cm under WL. The P saturation index (PSI) was 27.2% at 0–5 cm and 17.4% at 25–30 cm under FC, but averaged 11.9% at 0–30 cm under WL. We can conclude that extended WL associated with flooding creates reducing conditions in the soil, thus decreasing the concentration of Fe3+, but increasing the concentrations of Fe2+ and the solubility of P which can exacerbate the risk of P loss with runoff and leaching.
A Brief History of Broomcorn Millet Cultivation in Lithuania
Authors: Motuzaitė Matuzevičiūtė Giedrė , Laužikas Rimvydas
Published: August 2023

The eastern Baltic region represents the world’s most northerly limit of successful broomcorn millet (Panicum miliaceum) (hereafter, millet) cultivation in the past, yet this crop has been almost forgotten today. The earliest millet in the eastern Baltic region has been identified from macrobotanical remains which were directly dated to ca 1000 BCE. Between 800 and 500 BCE, millet was one of the major staple foods in the territory of modern-day Lithuania. Millet continued to play an important role in past agriculture up until the 15th century, with its use significantly declining during the following centuries. This paper analyses both the archaeobotanical records and written sources on broomcorn millet cultivation in Lithuania from its first arrival all the way through to the 19th century. The manuscript reviews the evidence of millet cultivation in the past as documented by archaeobotanical remains and historical accounts. In light of fluctuating records of millet cultivation through time, we present the hypothetical reasons for the decline in millet use as human food. The paper hypothesizes that the significant decrease in broomcorn millet cultivation in Lithuania from the 15th century onwards was likely influenced by several factors, which include climate change (the Little Ice Age) and the agricultural reforms of the 16th century. However, more detailed research is required to link past fluctuations in millet cultivation with climatic and historical sources, thus better understanding the roots of collapsing crop biodiversity in the past.
Representative Survey for Evaluating Housing and Manure Handling Technologies of the Hungarian Pig Sector
Authors: Benedek Zsuzsanna , Dublecz Károly , Koltay Ilona Anna , Fitos Gábor , Várhelyi Vanda Kisanna , Magyar Marianna , Pirkó Béla , Baranyai Nóra Hegedűsné
Published: August 2023

In Hungary, there is a lack of information on the pig production technologies in place in the base year of 2005 and changes since then, as well as a lack of information on the number of pigs kept in different age and production categories, which makes it difficult to calculate ammonia emissions and reductions in the national inventories. Our research team conducted a representative survey of pig farms to assess housing and manure management technologies in the Hungarian pig sector in 2005 and 2015. Novel expert-based calculation methods were developed to convert farm data on pig populations into daily average numbers (DAN) of animals in different statistical categories and feeding phases. The survey resulted in a representative database of housing, manure handling, storage and manure application practices in Hungarian pig production. The data and methodology from the survey helped to develop an ammonia emission calculator and knowledge transfer tool (AGEM-S) for use by farmers.
Supplementation of Freezing Medium with Ginseng Improves Rooster Sperm Quality and Fertility Relative to Free Radicals and Antioxidant Enzymes
Authors: Ratchamak Ruthaiporn , Authaida Supakorn , Koedkanmark Thirawat , Boonkum Wuttigrai , Semaming Yoswaris , Chankitisakul Vibuntita
Published: August 2023

To the best of our knowledge, this study is the first to determine the effect of ginseng as an antioxidant supplement in freezing extenders on the quality of cryopreserved rooster semen. Semen samples were collected from 40 Thai native roosters (Pradu Hang Dum) using the dorso-abdominal massage method and then pooled and divided into five groups according to the concentrations of ginseng supplementation (0, 0.25, 0.50, 0.75, and 1 mg/mL) in a freezing extender. The semen suspensions were loaded into a medium straw and cryopreserved using the liquid nitrogen vapor method. The post-thaw semen was evaluated for sperm quality (sperm motility and membrane integrity), seminal plasma characteristics (lipid peroxidation, superoxide dismutase [SOD], catalase [CAT], and glutathione peroxidase [GPx]), and fertility. The results showed that ginseng extract supplementation at 0.25 mg/mL yielded the highest total motility, progressive motility, and membrane integrity (59.47%, 30.82%, and 48.30%, respectively; p < 0.05) in cryopreserved rooster semen. Higher malondialdehyde concentrations were observed in the control group than in the other groups (p < 0.05). SOD, CAT, and GPx increased compared with those in the control group (p < 0.05). The results showed that the fertility rate with 0.25 mg/mL of ginseng was higher than that of the control group (62.80% vs. 46.28%: p < 0.05). In conclusion, supplementation with 0.25 mg/mL of ginseng is recommended as an alternative component to the freezing extender to improve rooster semen cryopreservation.
Ultrasonography and Sonoelastography Characteristics of Benign vs. Malignant Mesenteric Lymph Nodes in Cats: An Update
Authors: Febo Elettra , Del Signore Francesca , Bernabò Nicola , Paolini Andrea , Simeoni Francesco , De Bonis Andrea , Rosto Martina , Canal Sara , Vignoli Massimo
Published: August 2023

(1) Background: Strain elastography (SE) is an ultrasound-based technique able to non-invasively assess tissue elasticity, with malignant tissues being stiffer than normal tissues. The purpose of the study was to evaluate the diagnostic performance of SE to differentiate feline mesenteric benign and malignant lymph nodes (LNs) using a multivariate approach including both SE results and B-mode ultrasound and color Doppler findings. (2) Methods: Feline enlarged mesenteric LNs were evaluated using B-mode ultrasound, color Doppler ultrasonography, and SE. Short-to-long axis ratios, borders, echogenicity, hilum, vascular flow distribution, elastographic patterns, and strain ratios were recorded. Histological and/or cytological diagnosis was available for each LN. (3) Results: A total of 88 LNs were included, 46 (52.3%) benign and 42 (47.7%) malignant; in the benign group, 40 LNs had a diagnosis of reactive hyperplasia (group A) and 6 eosinophilic sclerosing lymphadenitis (group B), while in the malignant group 42 had a diagnosis of lymphoma (group C). The principal component analysis approach showed evidence that by combining B-mode- and color Doppler-based scores with SE scores, the three groups of LNs can be accurately distinguished. (4) Conclusions: Our results demonstrate that a multivariate sonographic approach combining B-mode ultrasound, color Doppler ultrasonography, and SE can accurately distinguish benign from malignant LNs, thus helping in the clinical advice of feline patients.
Exploring the Multifaceted Potential of a Peptide Fraction Derived from Saccharomyces cerevisiae Metabolism: Antimicrobial, Antioxidant, Antidiabetic, and Anti-Inflammatory Properties
Authors: Branco Patrícia , Maurício Elisabete Muchagato , Costa Ana , Ventura Diogo , Roma-Rodrigues Catarina , Duarte Maria Paula , Fernandes Alexandra R. , Prista Catarina
Published: August 2023

The rising demand for minimally processed, natural, and healthier food products has led to the search for alternative and multifunctional bioactive food components. Therefore, the present study focuses on the functional proprieties of a peptide fraction derived from Saccharomyces cerevisiae metabolism. The antimicrobial activity of the peptide fraction is evaluated against various foodborne pathogens, including Candida albicans, Candida krusei, Escherichia coli, Listeria monocytogenes, and Salmonella sp. The peptide fraction antioxidant properties are assessed using FRAP and DPPH scavenging capacity assays. Furthermore, the peptide fraction’s cytotoxicity is evaluated in colorectal carcinoma and normal colon epithelial cells while its potential as an antidiabetic agent is investigated through α-amylase and α-glucosidase inhibitory assays. The results demonstrate that the 2–10 kDa peptide fraction exhibits antimicrobial effects against all tested microorganisms, except C. krusei. The minimal inhibitory concentration for E. coli, L. monocytogenes, and Salmonella sp. remains consistently low, at 0.25 mg/mL, while C. albicans requires a higher concentration of 1.0 mg/mL. Furthermore, the peptide fraction displays antioxidant activity, as evidenced by DPPH radical scavenging activity of 81.03%, and FRAP values of 1042.50 ± 32.5 µM TE/mL at 1.0 mg/mL. The peptide fraction exhibits no cytotoxicity in both tumor and non-tumoral human cells at a concentration up to 0.3 mg/mL. Moreover, the peptide fraction presents anti-inflammatory activity, significantly reducing the expression of the TNFα gene by more than 29.7% in non-stimulated colon cells and by 50% in lipopolysaccharide-stimulated colon cells. It also inhibits the activity of the carbohydrate digestive enzymes α-amylase (IC50 of 199.3 ± 0.9 µg/mL) and α-glucosidase (IC20 of 270.6 ± 6.0 µg/mL). Overall, the findings showed that the peptide fraction exhibits antibacterial, antioxidant, anti-inflammatory, and antidiabetic activity. This study represents a step forward in the evaluation of the functional biological properties of S. cerevisiae bioactive peptides.
Managing Corneal Infections: Out with the old, in with the new?
Authors: Marasini Sanjay , Craig Jennifer P. , Dean Simon J. , Leanse Leon G.
Published: August 2023

There have been multiple reports of eye infections caused by antibiotic-resistant bacteria, with increasing evidence of ineffective treatment outcomes from existing therapies. With respect to corneal infections, the most commonly used antibiotics (fluoroquinolones, aminoglycosides, and cephalosporines) are demonstrating reduced efficacy against bacterial keratitis isolates. While traditional methods are losing efficacy, several novel technologies are under investigation, including light-based anti-infective technology with or without chemical substrates, phage therapy, and probiotics. Many of these methods show non-selective antimicrobial activity with potential development as broad-spectrum antimicrobial agents. Multiple preclinical studies and a limited number of clinical case studies have confirmed the efficacy of some of these novel methods. However, given the rapid evolution of corneal infections, their treatment requires rapid institution to limit the impact on vision and prevent complications such as scarring and corneal perforation. Given their rapid effects on microbial viability, light-based technologies seem particularly promising in this regard.
Microbiome–Metabolomic Analysis Reveals Beneficial Effects of Dietary Kelp Resistant Starch on Intestinal Functions of Hybrid Snakeheads (Channa maculata ♀ × Channa argus ♂)
Authors: Wang Shaodan , Zuo Zhiheng , Ye Bin , Zhang Li , Cheng Yanbo , Xie Shaolin , Zou Jixing , Xu Guohuan
Published: August 2023

The benefits of resistant starch on hypoglycemia, obesity prevention, antioxidant status and the alleviation of metabolic syndrome have received considerable attention. In this study, we explored how dietary kelp resistant starch (KRS) enhances intestinal morphology and function through a microbiome–metabolomic analysis. Hybrid snakeheads (initial weight: 11.4 ± 0.15 g) were fed experimental diets for 60 days. Fish were fed a basic wheat starch diet and the KRS diet. Dietary KRS improved intestinal morphology and enhanced intestinal antioxidant and digestive capabilities, as evidenced by decreased intestinal damage and upregulated intestinal biochemical markers. The microbiome analysis showed that KRS administration elevated the proportion of butyrate-producing bacteria and the abundance of beneficial bacteria that increases insulin sensitivity. Furthermore, significant alterations in metabolic profiles were observed to mainly associate with the amino acid metabolism (particularly arginine production), the metabolism of cofactors and vitamins, fat metabolism, glutathione metabolism, and the biosynthesis of other secondary metabolites. Additionally, alterations in intestinal microbiota composition were significantly associated with metabolites. Collectively, changes in intestinal microbiota and metabolite profiles produced by the replacement of common starch with dietary KRS appears to play an important role in the development of intestinal metabolism, thus leading to improved intestinal function and homeostasis.
A Stave-Aware Optical Music Recognition on Monophonic Scores for Camera-Based Scenarios
Authors: Liu Yipeng , Wu Ruimin , Wu Yifan , Luo Lijie , Xu Wei
Published: August 2023

The recognition of printed music sheets in camera-based realistic scenarios is a novel research branch of optical music recognition (OMR). However, special factors in realistic scenarios, such as uneven lighting distribution and curvature of staff lines, can have adverse effects on OMR models designed for digital music scores. This paper proposes a stave-aware method based on object detection to recognize monophonic printed sheet music in camera-based scenarios. By detecting the positions of staff lines, we improve the accuracy of note pitch effectively. In addition, we present the Camera Printed Music Staves (CPMS) dataset, which consists of labels and images captured by mobile phones under different angles and lighting conditions in realistic scenarios. We compare our method after training on different datasets with a sequence recognition method called CRNN-CTC on the test set of the CPMS dataset. The results show that the accuracy, robustness, and data dependency of our method perform better.
Determination of Vehicle Emission Rates for Ammonia and Organic Molecular Markers Using a Chassis Dynamometer
Authors: Yu Geun-Hye , Song Myoung-Ki , Oh Sea-Ho , Choe Seo-Yeong , Kim Min-Wook , Bae Min-Suk
Published: August 2023

Stringent regulations have been implemented to address vehicle exhaust emissions and mitigate air pollution. However, the introduction of exhaust gas reduction devices, such as Three-Way Catalytic converters, has raised concerns about the generation and release of additional pollutants such as NH3. This study utilized a chassis dynamometer to investigate the characteristics of exhaust pollutants, including carbon monoxide (CO), hydrocarbons (HC), nitrogen oxides (NOx), particulate matter (PM), ammonia (NH3), organic carbon (OC), and elemental carbon (EC). The emissions were examined across various vehicle fuel types, namely liquefied petroleum gas, gasoline, and diesel (EURO4, EURO6), to assess their individual contributions to exhaust emissions. The results revealed significant variations in the emission levels of regulated pollutants (CO, HC, NOx, and PM) during driving, depending on factors such as engine technology, emissions control strategies, fuel type, and test cycle. Notably, NH3 emissions analysis according to driving mode indicated that gasoline vehicles exhibited the highest NH3 emissions, while diesel vehicles emitted negligible amounts. This observation can be attributed to the production of NH3 as a byproduct of catalytic reduction processes implemented by exhaust gas reduction devices targeting CO, HC, and NOx. In addition, EURO4 vehicles demonstrated higher emission levels of OC and EC compared with other fuel types. Furthermore, the presence of diesel particulate filters (DPFs) in diesel vehicles effectively reduced PM emissions. Moreover, this study investigated the emission characteristics of organic molecular markers within the organic carbon fraction, revealing distinct emission profiles for each vehicle and fuel type. These findings contribute to the identification of emission sources by discerning the primary components emitted by specific fuel types.
A Multi-Bandwidth Reconfigurable Patch Antenna for Devices in WLAN and UWB Technology Applications
Authors: Bikrat Mohamed , Bri Seddik , Bravo Alberto Gascón , Manterola Alejandro Muñoz , Gonzalez-Atienza Miriam , Amador Farah
Published: August 2023

This article introduces a process to design, simulate, and measure a novel multi-band patch antenna with different operation modes, i.e., band centers and bandwidths. Switching between operation modes is possible using a pair of PIN diodes to connect different parts of the antenna with the main antenna patch. Such a reconfigurable design allows for individual control of each frequency range. The main operation mode of the resulting antenna has an impedance bandwidth with two bands, one from 2.4 GHz to 2.73 GHz and another from 3.4 GHz to 5.73 GHz, with a maximum gain of 4.85 dBi and stable radiation patterns. The resulting antenna is suitable for applications using both ultra-wideband technologies and wireless local-area network (WLAN) technologies.
Immediate versus Delayed Implant Placement in Patients with Tooth Agenesis: An In-Line Retrospective Pilot Study Comparing Clinical and Patient-Related Outcomes
Authors: Alexandre Perez , Hamzah Shabana , Lombardi Tommaso
Published: August 2023

This retrospective study compared clinical, radiological, and patient- and clinician-reported outcomes between immediate and delayed implants placed in patients with tooth agenesis as part of one-stage implant therapy with a split-mouth design. A total of 12 sites of permanent tooth agenesis in five patients received 12 implants (2.4 implants per patient), six being immediate implants in deciduous teeth post-extraction sites, and six being late implants replacing already extracted or spontaneously lost deciduous teeth. Data reporting was performed descriptively without statistical comparative analysis between study groups. Radiographic crestal bone level changes between the time of surgery and 1-year post-surgery did not indicate any marked difference between treatment groups. Patient-reported outcomes related to post-surgical pain and patient preference indicated less pain in immediately treated sites, resulting in a corresponding patient preference related to this treatment modality. Clinician-rated satisfaction levels did not differ. The results of this pilot study support the use of immediate implant placement as a suitable procedure in cases of tooth agenesis.
Test Trials and Analysis of Pod-Shattering Characteristics of Harvested Rapeseed Silique
Authors: Zhang Min , Li Gang , Yang Yao , Jin Mei , Wang Gang
Published: August 2023

In order to reduce the silique shattering loss of the rapeseed mechanical harvesting process, based on the state of force on the silique during the rapeseed harvesting reel branch stage, Ningza 1810, Zhenyou 8, and Fengyou 306 were used as research objects, and the experimental research on the factors affecting rapeseed silique shattering was carried out using the swing impact method. The experimental analysis showed that rapeseed varieties, silique moisture content, silique growth position, collision material, impact speed, force position, and other factors had significant effects on silique shattering. The impact velocity was less than 1.5 m·s−1, the difference in the effect of each factor on pod shattering was not significant, and it was not easy to shatter when the moisture content of the rapeseed silique was higher. The impact resistance of the front side of rapeseed was two to four times that of the bonding surface of rapeseed petals, the shattering rate of the top rapeseed silique was twice that of the bottom siliques, and when siliques were supported, they were more likely to shatter under external forces than when they were unsupported. The experimental study of the mechanical properties of rapeseed siliques was carried out using the impending fracture method; the experimental analyses showed that the support position and force position of the silique, the loading speed, and the growth position of the silique had a significant effect on the mechanical properties of the silique. The maximum cracking force was higher and the bending strength was stronger when the body of the silique was supported; the range of the maximum cracking force was 3.05 N to 4.16 N, and the bending strength range was 8.48 MPa to 11.57 MPa. The maximum cracking force and bending strength of the silique were stronger when the front side of the silique petal was pressurized than when the bonding surface of the petal was pressurized. Based on Pearson’s correlation and grey correlation analysis, the morphological characteristics of rapeseed siliques were ranked in order of their influence on the performance of siliques in terms of the angle between the silique and stalk, stalk diameter, petal thickness, beak length, silique thickness, silique width, and silique length. This study can be used as a reference for the design and optimization of the rapeseed harvesting reel branch mechanism and the selection of machine-harvestable rapeseed varieties.
Study the Effect of Operating Temperature on Performance in Dry Scroll Vacuum Pump
Authors: Zhang Ying-Li , Yue Xiang-Ji , Ding Jia-Nan , Ba De-Chun
Published: August 2023

The dry scroll vacuum pump is a kind of variable capacity dry vacuum pump that is applied in chemical, pharmaceutical, semiconductor, and scientific instruments. Analyzing the thermodynamic characteristics of the scroll vacuum pump and studying the impact of operating temperature on its consistent performance are necessary for the design and application of the cooling system of the scroll vacuum pump. Based on the dynamics mesh method, the computational fluid dynamics (CFD) simulation model of a multi-stage dry scroll vacuum pump is established in this study. Under the suction pressure of 1000 Pa, the simulation calculation is carried out at different operating temperatures of fixed scrolls (25 °C, 45 °C, and 65 °C). The results show that the pumping speed, the gas compression power, and the energy efficiency ratio are significantly affected by different temperatures. The operating temperature of the pump has been reduced from 65 °C to 25 °C, the pumping speed is increased by approximately 5.97%, the gas compression power is increased by approximately 4.32%, and the energy efficiency ratio is increased by approximately 1.745%. This research provides a transient simulation method using dynamic mesh technology for the study of the dry scroll vacuum pump, and the results can provide a theoretical basis for the design and application of the cooling system of the dry scroll vacuum pump.
Intelligent Design of Hairpin Filters Based on Artificial Neural Network and Proximal Policy Optimization
Authors: Ye Yunong , Wu Yifan , Chen Jiayu , Su Guodong , Wang Junchao , Liu Jun
Published: August 2023

Microstrip filters are widely used in high-frequency circuit design for signal frequency selection. However, designing these filters often requires extensive trial and error to achieve the desired performance metrics, leading to significant time costs. In this work, we propose an automated design flow for hairpin filters, a specific type of microstrip filter. We employ artificial neural network (ANN) modeling techniques to predict the circuit performance of hairpin filters, and leverage the efficiency of low-cost models to deploy reinforcement learning agents. Specifically, we use the proximal policy optimization (PPO) reinforcement learning algorithm to learn abstract design actions for the filters, allowing us to achieve automated optimization design. Through simulation results, we demonstrate the effectiveness of the proposed approach. By optimizing the geometric dimensions, we significantly improve the performance metrics of hairpin filters, and the trained agent successfully meets our specified design goals within 5 to 15 design steps. This work serves as a conceptual validation attempt to apply reinforcement learning techniques and pre-trained ANN models to automate MMIC filter design. It exhibits clear advantages in terms of time-saving and performance efficiency when compared to other optimization algorithms.
Body-Pose-Guided Action Recognition with Convolutional Long Short-Term Memory (LSTM) in Aerial Videos
Authors: Saeed Sohaib Mustafa , Akbar Hassan , Nawaz Tahir , Elahi Hassan , Khan Umar Shahbaz
Published: August 2023

The accurate detection and recognition of human actions play a pivotal role in aerial surveillance, enabling the identification of potential threats and suspicious behavior. Several approaches have been presented to address this problem, but the limitation still remains in devising an accurate and robust solution. To this end, this paper presents an effective action recognition framework for aerial surveillance, employing the YOLOv8-Pose keypoints extraction algorithm and a customized sequential ConvLSTM (Convolutional Long Short-Term Memory) model for classifying the action. We performed a detailed experimental evaluation and comparison on the publicly available Drone Action dataset. The evaluation and comparison of the proposed framework with several existing approaches on the publicly available Drone Action dataset demonstrate its effectiveness, achieving a very encouraging performance. The overall accuracy of the framework on three provided dataset splits is 74%, 80%, and 70%, with a mean accuracy of 74.67%. Indeed, the proposed system effectively captures the spatial and temporal dynamics of human actions, providing a robust solution for aerial action recognition.
Development and Evaluation of a Tethered Class C3 Hexacopter in Maritime Conditions on the Helipad of a Ferry
Authors: Kownacki Cezary , Ambroziak Leszek , Ciężkowski Maciej , Wolniakowski Adam , Romaniuk Sławomir , Kulesza Zbigniew , Bożko Arkadiusz , Ołdziej Daniel
Published: August 2023

Various unmanned aerial vechicle (UAV) applications, especially those based on reconnaissance and observation missions, often require an unlimited time of flight. This is possible only when a UAV is continuously supplied with power from a ground-based power source, which is why tethered UAV systems were developed. Tethered UAV systems are based on multicopters, which can hover above a landing pad or track its position if it is movable. The presented research concerned the development of a large C3 class hexacopter with a maximal payload of about 1 kg and a takeoff mass of 16 kg, which was tested in maritime conditions during a ferry’s cruise. The main purpose of the hexacopter was to continuously observe the area ahead of the vessel to detect and localize obstacles in the water. During the experimental tests, critical phases of flight were identified, the AC/DC power supply unit and power cord unwinder were tested, and the power required by six brushless direct current (BLDC) motors was registered. The obtained results could be useful in future work on tethered UAV systems applied in windy maritime conditions.
MT-SOTA: A Merkle-Tree-Based Approach for Secure Software Updates over the Air in Automotive Systems
Authors: Bazzi Abir , Shaout Adnan , Ma Di
Published: August 2023

The automotive industry has seen a dynamic transformation from traditional hardware-defined to software-defined architecture enabling higher levels of autonomy and connectivity, better safety and security features, as well as new in-vehicle experiences and richer functions through software and ongoing updates of both functional and safety-critical features. Service-oriented architecture plays a pivotal role in realizing software-defined vehicles and fostering new business models for OEMs. Such architecture evolution demands new development paradigms to address the increasing complexity of software. This is crucial to guarantee seamless software development, integration, and deployment—all the way from cloud or backend repositories to the vehicle. Additionally, it calls for enhanced collaboration between car manufacturers and suppliers. Simultaneously, it introduces challenges associated with the necessity for ongoing updates and support ensuring vehicles remain safe and up to date. Current approaches to software updates have primarily been implemented for traditional vehicle architectures, which mostly comprise specialized electronic control units (ECUs) designed for specific functions. These ECUs are programmed with a single comprehensive executable that is then flashed onto the ECU all at once. Different approaches should be considered for new software-based vehicle architectures and specifically for ECUs with multiple independent software packages. These packages should be updated independently and selectively for each ECU. Thus, we propose a new scheme for software updates based on a Merkle tree approach to cope with the complexity of the new software architecture while addressing safety and security requirements of real-time and resource-constrained embedded systems in the vehicle. The Merkle-tree-based software updates over the air (MT-SOTA) proposal enables secure updates for individual software clusters. These clusters are developed and integrated by diverse entities with varying release timelines. Our study demonstrates that the MT-SOTA scheme can enhance the speed of software update execution without significantly increasing the process overhead. Additionally, it offers necessary defense against potential cyberthreats. The results of the performed technical analysis and experiments of the MT-SOTA implementation are presented in this paper.
Development and Optimisation of Hydrogel Scaffolds for Microvascular Network Formation
Authors: Fuenteslópez Carla V. , Thompson Mark S. , Ye Hua
Published: August 2023

Traumatic injuries are a major cause of morbidity and mortality worldwide; however, there is limited research on microvascular traumatic injuries. To address this gap, this research aims to develop and optimise an in vitro construct for traumatic injury research at the microvascular level. Tissue engineering constructs were created using a range of polymers (collagen, fibrin, and gelatine), solvents (PBS, serum-free endothelial media, and MES/NaCl buffer), and concentrations (1–5% w/v). Constructs created from these hydrogels and HUVECs were evaluated to identify the optimal composition in terms of cell proliferation, adhesion, migration rate, viability, hydrogel consistency and shape retention, and tube formation. Gelatine hydrogels were associated with a lower cell adhesion, whereas fibrin and collagen ones displayed similar or better results than the control, and collagen hydrogels exhibited poor shape retention; fibrin scaffolds, particularly at high concentrations, displayed good hydrogel consistency. Based on the multipronged evaluation, fibrin hydrogels in serum-free media at 3 and 5% w/v were selected for further experimental work and enabled the formation of interconnected capillary-like networks. The networks formed in both hydrogels displayed a similar architecture in terms of the number of segments (10.3 ± 3.21 vs. 9.6 ± 3.51) and diameter (8.6446 ± 3.0792 μm vs. 7.8599 ± 2.3794 μm).