bluuebunny/crossref_metadata_2025 huggingface dataset:
Intraspecific phylogeny and genomic resources development for an important medical plant Dioscorea nipponica, based on low-coverage whole genome sequencing data
Authors: Hu Ke , Chen Min , Li Pan , Sun Xiaoqin , Lu Ruisen
Published: December 2023

Dioscorea nipponica Makino, a perennial twining herb with medicinal importance, has a disjunctive distribution in the Sino-Japanese Floristic Region. It has a long history in traditional Chinese medicine, with demonstrated efficacy against various health conditions. However, the limited genomic data and knowledge of genetic variation have hindered its comprehensive exploration, utilization and conservation. In this study, we undertook low-coverage whole genome sequencing of diverse D. nipponica accessions to develop both plastome (including whole plastome sequences, plastome-derived SSRs and plastome-divergent hotspots) and nuclear genomic resources (including polymorphic nuclear SSRs and single-copy nuclear genes), as well as elucidate the intraspecific phylogeny of this species. Our research revealed 639 plastome-derived SSRs and highlighted six key mutational hotspots (namely CDS ycf1, IGS trnL-rpl32, IGS trnE-trnT, IGS rps16-trnQ, Intron 1 of clpP, and Intron trnG) within these accessions. Besides, three IGS regions (i.e., ndhD-cssA, trnL-rpl32, trnD-trnY), and the intron rps16 were identified as potential markers for distinguishing D. nipponica from its closely related species. In parallel, we successfully developed 988 high-quality candidate polymorphic nuclear SSRs and identified 17 single-copy nuclear genes for D. nipponica, all of which empower us to conduct in-depth investigations into phylogenetics and population genetics of this species. Although our phylogenetic analyses, based on plastome sequences and single-copy nuclear genes revealed cytonuclear discordance within D. nipponica, both findings challenged the current subspecies classification. In summary, this study developed a wealth of genomic resources for D. nipponica and enhanced our understanding of the intraspecific phylogeny of this species, offering valuable insights that can be instrumental in the conservation and strategic utilization of this economically significant plant.
The multilevel correlates, contributions, and consequences of leader humility in humanitarian aid work
Authors: Davis Edward B. , Barneche Kelly , Aten Jamie D. , Shannonhouse Laura R. , Wang David C. , Van Tongeren Daryl R. , Davis Don E. , Hook Joshua N. , Chen Zhuo Job , Lefevor G. Tyler , McElroy-Heltzel Stacey E. , Elick Emilie L. , Van Grinsven Leif , Lacey Ethan K. , Brandys Tyler R. , Sarpong Philip K. , Osteen Sophia A. , Shepardson Kati
Published: December 2023

Objective

Leader humility has been linked to many positive outcomes but not examined in humanitarian aid work. Three studies examined the multilevel correlates, contributions, and consequences of leader humility in Medair—a large, multinational, faith-based aid organization. Study 1 examined correlates of leader humility in a sample of 308 workers and 167 leaders. Study 2 explored multilevel contributions of leader humility in 96 teams comprised of 189 workers. Study 3 utilized a subsample (50 workers, 34 leaders) to explore consequences of Time 1 leader and team humility on outcomes 6 months later.

Method

Participants completed measures of humility (general, relational, team), leader and team attributions (e.g., effectiveness, cohesion, and growth-mindedness), organizational outcomes (e.g., job engagement and satisfaction; worker and team performance), and psychological outcomes (e.g., depression, anxiety, compassion satisfaction, and flourishing).

Results

Leader and team humility contributed to multilevel positive attributions about leaders (as effective and impactful), teams (as cohesive, psychologically safe, and growth-minded), and oneself (as humble), and those attributions contributed to organizational and psychological outcomes. Teams’ shared attributions of their leader’s humility contributed to higher worker job satisfaction and team performance. Longitudinally, for workers and leaders, leader and team humility were associated with some positive organizational and psychological outcomes over time.

Conclusion

In humanitarian organizations, leader humility seems to act as an attributional and motivational social contagion that affects aid personnel’s positive attributions about their leaders, teams, and themselves. In turn, these multilevel positive attributions contribute to several positive team, organizational, and psychological outcomes among workers and leaders.
Socio-ecological drivers of vulnerabilities of children living within orphan homes and the implications for their nurturance care
Authors: Onayemi Olayinka M. , Hapunda Given
Published: December 2023

At the heart of the Sustainable Development Goals (SDG) is the vision to “leave no one behind, and to see that all children survive, thrive and transform. However, some categories of children may remain left behind owing to their disproportionate exposure to the risk of threats and deficit of attention to the social and ecological climate that characterizes the various systems in which they are found. This study is concerned with one major question: Despite diverse local and international instruments that favor full nurturance and development of children, what social forces play as threat to full nurturance care in the context of children living in Orphan homes? Nurturing care framework and Brofenbrener’s ecological system theory were adopted as the analytical frameworks. Research design was exploratory. Data were collected through sessions of in-depth-interview with orphanage managers, caregivers, and social workers on the socio-ecology drivers of threat to children living within the orphan home space and its implications for nurturance care across the various complex systems of the child’s environment. The study found various factors across the complex systems of child development – microsystem, mesosystem, exosystem, microsysm and lastly, chronosystem- which undermine caregivers’ delivery and increases children’s vulnerability and risk of missing out on effective nurturance care. These vulnerabilities are endemic realities of social, and bio-ecologcal space in which child development occurs. This study recommends specialized interventions and policy directives relevant for each identified threat. It also calls for a stronger political will in improving the conditions of this category of the children while within the orphan home space and ultimately, actions towards deinstitutionalization of children.
Baseline and early changes in laboratory parameters predict disease severity and fatal outcomes in COVID-19 patients
Authors: Gize Addisu , Belete Yerega , Kassa Melkayehu , Tsegaye Wondewosen , Hundie Gadissa Bedada , Belete Birhan Mesele , Bekele Mahteme , Ababaw Berhan , Tadesse Yosef , Fantahun Bereket , Sirgu Sisay , Ali Solomon , Tizazu Anteneh Mehari
Published: December 2023

Introduction

Coronavirus disease 2019 (COVID-19) has become the worst catastrophe of the twenty-first century and has led to the death of more than 6.9 million individuals across the globe. Despite the growing knowledge of the clinicopathological features of COVID-19, the correlation between baseline and early changes in the laboratory parameters and the clinical outcomes of patients is not entirely understood.

Methods

Here, we conducted a time series cross-sectional study aimed at assessing different measured parameters and socio-demographic factors that are associated with disease severity and the outcome of the disease in 268 PCR-confirmed COVID-19 Patients.

Results

We found COVID-19 patients who died had a median age of 61 years (IQR, 50 y – 70 y), which is significantly higher (p < 0.05) compared to those who survived and had a median age of 54 years (IQR, 42y – 65y). The median RBC count of COVID-19 survivors was 4.9 × 106/μL (IQR 4.3 × 106/μL – 5.2 × 106/μL) which is higher (p < 0.05) compared to those who died 4.4 × 106/μL (3.82 × 106/μL – 5.02 × 106/μL). Similarly, COVID-19 survivors had significantly (p < 0.05) higher lymphocyte and monocyte percentages compared to those who died. One important result we found was that COVID-19 patients who presented with severe/critical cases at the time of first admission but managed to survive had a lower percentage of neutrophil, neutrophil to lymphocyte ratio, higher lymphocyte and monocyte percentages, and RBC count compared to those who died.

Conclusion

To conclude here, we showed that simple laboratory parameters can be used to predict severity and outcome in COVID-19 patients. As these parameters are simple, inexpensive, and radially available in most resource-limited countries, they can be extrapolated to future viral epidemics or pandemics to allocate resources to particular patients.
Exploring the microbial landscape: uncovering the pathogens associated with community-acquired pneumonia in hospitalized patients
Authors: Hansen Karin , Yamba Yamba Linda , Wasserstrom Lisa , Rünow Elisabeth , Göransson Tommy , Nilsson Anna , Ahl Jonas , Riesbeck Kristian
Published: December 2023

Objectives

This study aimed to investigate the etiology, clinical features, and outcomes of community-acquired pneumonia (CAP) in adults. Understanding the causative pathogens is essential for effective treatment and prevention.

Design

Between 2016–2018, 518 hospitalized adults with CAP and 241 controls without symptoms were prospectively enrolled. Urine samples were collected for pneumococcal urinary antigen tests and nasopharyngeal swabs for viral and bacterial analysis, combined with routine diagnostic care.

Results

Among the included CAP patients, Streptococcus pneumoniae was the most common pathogen, detected in 28% of patients, followed by Haemophilus influenzae in 16%. Viruses were identified in 28%, and concurrent viruses and bacteria were detected in 15%. There was no difference in mortality, length of stay, or symptoms at hospitalization when comparing patients with bacterial, viral, or mixed etiologies. Among the control subjects without respiratory symptoms, S. pneumoniae, H. influenzae, or Moraxella catarrhalis were detected in 5–7%, and viruses in 7%.

Conclusion

Streptococcus pneumoniae emerged as the predominant cause of CAP, followed closely by viruses and H. influenzae. Intriguingly, symptoms and outcome were similar regardless of etiology. These findings highlight the complexity of this respiratory infection and emphasize the importance of comprehensive diagnostic and treatment strategies.

Clinical Trial Registration: ClinicalTrials.gov, identifier [NCT03606135].
Wellbeing-responsive community: a growth target for intentional mental health promotion
Authors: Raymond Ivan J. , Burke Karena J. , Agnew Kylie J. , Kelly David M.
Published: December 2023

With mental illness remaining a significant burden of disease, there is an ongoing need for community-based health promotion, prevention, and responses (or “mental health promotion activities”). The health promotion, community development, and positive psychology literature identifies significant heterogeneity in the design and delivery of these activities. This variability spans: (1) individual vs. group outcomes, (2) psychological vs. sociological determinants of change, (3) promoting wellbeing vs. reducing mental health symptoms, and (4) the degree activities are contextualized vs. standardized in design and delivery. Mental health promotion activities do not easily accomplish this level of complexity within design and implementation. This has led to the emergence of the complexity-informed health promotion literature and the need for innovative tools, methods, and theories to drive this endeavor. This article directly responds to this call. It introduces “wellbeing-responsive community”: a vision and outcome hierarchy (or growth target) for intentionally delivered mental health promotion. The construct enables the design and implementation of interventions that intentionally respond to complexity and contextualization through the drivers of co-creation, intentionality, and local empowerment. It represents a community (support team, programme, agency, network, school, or region) that has the shared language, knowledge, methods, and skills to work together in shared intent. In other words, to integrate best-practice science with their local knowledge systems and existing strengths, and intentionally co-create and deliver contextualized wellbeing solutions at both the individual and community levels that span the “system” (e.g., whole-of-community) to the “moment” (e.g., intentional support and care). Co-creation, as applied through a transdisciplinary lens, is emerging as an evidence-based method to respond to complexity. This article describes the rationale and evidence underpinning the conceptualization of a wellbeing-responsive community through the integration of three key disciplines: (1) positive psychology, (2) ecological or systems approaches, and (3) intentional practice (implementation science). A definitional, contextual, and applied overview of the wellbeing-responsive community is provided, including a hierarchy of outcomes and associated definitions. Its purported application across education, mental health, community service, and organizational settings is discussed, including its potential role in making complexity-informed health promotion practical for all knowledge users.
Uses of Andersen health services utilization framework to determine healthcare utilization for mental health among migrants—a scoping review
Authors: Krzyż Ewa Zuzanna , Antunez Martinez Oscar Fidel , Lin Hung-Ru
Published: December 2023

Background

Migration is a worldwide occurrence that carries significant implications for healthcare systems, and it entails challenges to mental healthcare. The Andersen Behavioral Model is widely used by researchers to determine healthcare service utilization among many populations, including migrants. Our study aimed to explore the ways of using the Andersen Health System Utilization Framework in the literature to discover the utilization of mental healthcare by migrants.

Methods

This scoping review was based on Arksey and O’Malley’s framework. A comprehensive search was performed across five electronic databases.

Results

A total of 12 articles from January 1992 to July 2023 identified various versions of the Andersen Behavioral Model to provide an overview of mental health services utilization among migrants. The analysis identified four significant trends in the literature. First, there is a predominant focus on individual characteristics over contextual factors. Second, researchers tend to integrate multiple versions of the Andersen Behavioral Model, and the most is the version from 1995. Third, additional factors specific to migrant populations are incorporated into the model, but the categorization is sometimes unclear. Finally, the majority of studies have used a quantitative approach and are based in North America, suggesting a focus on the significance of mental health in migrant communities in that context.

Conclusion

In summary, our scoping review calls for further research using the Andersen Behavioral Model to study mental healthcare utilization among migrants. Notable findings include the adaptation of the model to migrant populations, a focus on individual characteristics, a need for more diverse research methods, and the proposal of a new conceptual model to guide research and policy development in this field.
The determinants of public acceptance of telemedicine apps: an innovation diffusion perspective
Authors: Liu Dong , Son Sangbum , Cao Junwei
Published: December 2023

With the rapid advancement of information technology, telemedicine apps have gradually become an indispensable tool for providing patients with more convenient, efficient, and accessible healthcare services. However, the successful implementation of these apps largely depends on widespread acceptance among the public. To thoroughly investigate the factors influencing the public’s acceptance of these apps and the relationships between these factors, this study developed a theoretical model based on the Diffusion of Innovation theory and the Theory of Perceived Value. To validate this model, we conducted a survey of 387 residents in Beijing, China, and employed structural equation modeling to analyze the collected data. The research findings indicate that attributes of innovation diffusion, including relative advantage, compatibility, complexity, trialability, and observability, significantly and positively influence the public’s perceived value. Particularly noteworthy is that perceived value partially mediates the relationship between innovation attributes and public acceptance, emphasizing the crucial role of perceived value in the public decision-making process. This study employed a theory-driven approach to elucidate the acceptance of telemedicine apps and offers fresh insights into the existing literature. By integrating the research paradigms of innovation diffusion and customer perceived value, we provide a coherent explanation of how individual cognitive processes lead to acceptance behavior. In summary, this research enriches the existing theoretical studies on the acceptance of telemedicine apps and holds positive implications for healthcare practice.
Electron diffusion by chorus waves: effects of latitude-dependent wave power spectrum
Authors: Yu Jiang , Wang Jing , He Zhaoguo , Chen Zuzheng , Li Liuyuan , Cui Jun , Cao Jinbin
Published: December 2023

In the present paper, we investigate the effects of latitude-dependent wave power spectrum on the interactions of chorus with electrons. Great errors in evaluating the electron diffusion coefficients and the resultant electron temporal evolutions are introduced by the widely adopted latitudinally constant model, compared with the latitudinally varying model. The latitudinally constant model tends to overestimate (underestimate) the diffusion coefficients for electrons below (above) 200 keV. The overestimation and underestimation are mainly confined in small to intermediate pitch angles, increase with decreasing pitch angles, and can reach up to several orders of magnitude. The large differences in diffusion coefficients significantly alter the net changes of electron phase space densities and the resultant shapes of electron pitch angle distributions. Our simulations demonstrate that the wave power spectrum distribution along the magnetic field line plays an important role in controlling the dynamics of radiation belt electrons.
Association between daily screen time and risk of stroke among middle-aged and elderly people: research based on China health and nutrition survey
Authors: Ren Yaxin , Sun Kejuan , Rong Yueqing , Song Shiming , Zhai Yijing , Miao Junjie , Shi Hongmei , Xue Hongmei , Li Zengning
Published: December 2023

Background

We aimed to explore the independent associations between screen time and the risk of stroke among Chinese adults based on the China Health and Nutrition Survey (CHNS).

Methods

Data on Chinese adults aged older than 40 years from the CHNS in during 2004–2009 were selected. A total of 4,587 individuals were included in 2009, including screen time and the risk of stroke. Simultaneously, we traced the previous screen time to 2004 for those with outcome measures in 2009 (n = 2,100). Basic information, lifestyle, and screen behavior were obtained through face-to-face interviews and self-completed questionnaires. Anthropometric data collected included blood pressure, body weight, height, hip circumference, and waist circumference. Fasting blood was obtained for measurements of lipid and glucose levels. Cross-sectional analysis and cohort analysis were both performed using multivariate logistic regression.

Results

Of all participants, 3,004 (65.49%) participants spent more than 2 h per day on screen time. Taking the men who spent less than 2 h on screen per day as reference, the crude odds ratio (OR) of the high risk of stroke was 1.53 [95% confidence interval (CI), 1.20–1.95] for the men who spent 2–3 h per day on screen and 2.37 (95% CI, 1.78–3.16) for the men who spent more than 3 h per day on screen. This difference remained significant after adjusting for confounding factors. No association was observed among women. However, in the cohort analysis with screen time in 2006 as the independent variable, the association between screen time and stroke risk was found both in men [OR, 1.83 (95% CI, 1.19–2.82)] and women [OR, 1.48 (95% CI, 1.10–1.99)]).

Conclusion

We found that the high screen time was associated with an increased stroke risk, which was pronounced in men, warranting a universal need to limit screen time in order to improve health.
Algae as a source of protein in the sustainable food and gastronomy industry
Authors: Procházka Petr , Abrham Josef , Cerveny Jaroslav , Soukupová Jana , Ouma Christine Nabwire , Mullen Kevin Jan , Sanova Petra , Smutka Lubos
Published: December 2023

Introduction

The paper aims to examine the prospects of algae production as a source of protein in the European market. As well as highlighting the promising developments in the algae food industry in Europe. By 2027, it is expected that the algae protein market will be worth approximately USD 300 million.

Methods

We conducted thematic analysis and synthesis of scientific literature and conceptual documents at the European level.

Results

The studies reviewed show that the nutritional value of food products can be increased by using algae. The production of algae for food should be encouraged in Europe because it is a viable alternative protein source. To fully utilize algae as a source of protein, however, a number of technological, regulatory, and market-related obstacles must be resolved despite the indicative advantages. Developing cost-effective and efficient methods for algae cultivation, harvesting, and processing are also necessary. Uniform and consistent regulations are needed to guarantee the safety and quality of products that contain algae, as well as consumer awareness campaigns and education about the advantages of algae protein.

Conclusion

In addition to providing evidence of the viability of algae production as a source of food, this study demonstrates that algae land needs are negligible compared to other protein sources—animal-based like pork, chicken and beef production but also plant-based alternatives such as nuts, pulses, grains and peas. Furthermore, results in this study may inspire a more targeted focus on algae production as a source of nutrition and inspire more organizations around the world to move ahead with the alternative protein source production from algae.
Molecular genetic characterization and meat-use functional gene identification in Jianshui yellow–brown ducks through combined resequencing and transcriptome analysis
Authors: Li Xinpeng , Xin Aiguo , Ma Li , Gou Xiao , Fang Suyun , Dong Xinxing , Ni Bin , Tang Lin , Zhu Li , Yan Dawei , Kong Xiaoyan
Published: December 2023

The Jianshui yellow–brown duck is a unique country-specific waterfowl species in Yunnan Province, well known for its tender meat. However, there is a lack of comprehensive systematic research on the molecular genetic characteristics, especially germplasm resources and economic traits, of the Jianshui yellow–brown ducks. This study investigated the molecular genetic characteristics of Jianshui yellow–brown ducks, compared their selection signals with those of ancestral mallard and meat-type Pekin ducks, and identified genes specific to their meat-use performance. Furthermore, this study also evaluated the breeding potential for its meat performance. In this study, phylogenetic trees, PCA and Admixture analysis were used to investigate the population genetic structure among local duck breeds in China; population genetic differentiation index (Fst), nucleotide diversity and Tajima’s D were used to detect selected loci and genes in the population of Jianshui yellow–brown ducks; and transcriptome technology was used to screen for differentially expressed genes in the liver, sebum and breast muscle tissues, and finally, the results of the genome selection signals and transcriptome data were integrated to excavate functional genes affecting the meat performance of the Jianshui yellow–brown ducks. The results of the genetic structure of the population showed that Jianshui yellow–brown ducks were clustered into a separate group. Selection signal analysis indicated significant selection pressure on certain genes related to meat characteristics (ELOVL2, ELOVL3, GDF10, VSTM2A, PHOSPHO1, and IGF2BP1) in both Jianshui yellow–brown ducks and mallards. Transcriptomic data analysis suggested that ELOVL3, PHOSPHO1, and GDF10 are vital candidate genes influencing meat production and quality in Jianshui yellow–brown ducks. A comparison of selection signals between Jianshui yellow–brown ducks and Pekin ducks revealed only 21 selected genes in the Jianshui yellow–brown duck population, and no significant genes were related to meat traits. Moreover, whole-genome resequencing data suggested that the Jianshui yellow–brown duck represents a unique category with distinct genetic mechanisms. Through selection signaling and transcriptomic approaches, we successfully screened and identified important candidate genes affecting meat traits in Jianshui yellow–brown ducks. Furthermore, the Jianshui yellow–brown duck has good potential for improved meat performance, highlighting the need for further improvement.
Supplementation of dietary areca nut extract modulates the growth performance, cecal microbiota composition, and immune function in Wenchang chickens
Authors: Wang Shiping , Wang Hong , Jiang Qicheng , Dai Jiahui , Dai Wenting , Kang Xiaoning , Xu Tieshan , Zheng Xinli , Fu An , Xing Zengyang , Chen Yiyong , He Zhongchun , Lu Lizhi , Gu Lihong
Published: December 2023

Introduction

The study was aimed at evaluating the effects of areca nut extract (ANE) on the growth performance, cecal microbiota, and immunity of Wenchang chickens.

Methods

For this study, 42-day-old healthy Wenchang chickens (n = 450) with similar body weight were chosen. The animals were randomly divided into five groups, with six replicates per group and 15 chickens per replicate. One group was fed a basal diet (control; CCK). The remaining four groups were fed a basal diet supplemented with varying ANE concentrations: 0.038, 0.063, 0.100, and 0.151 g/kg, with the groups denoted as CNT1, CNT2, CNT3, and CNT4, respectively. The feeding experiment lasted 35 days. The ligated cecum segments of the control and experimental groups were collected for metabolomic and metagenomic analysis, while the bone marrow samples were extracted for tandem mass tag (TMT)-based proteomic analysis.

Results

All the experimental groups exhibited significantly higher average daily gain (ADG) and significantly lower feed-to-weight (F/G) ratios than CCK. Metabolomic screening of the cecum contents revealed the presence of 544 differential metabolites, including several gut health–related metabolites, such as xanthine, hydroxy hypoxanthine, 2,5-dimethylhydrazine, ganoderic acid, and 2-aminohexanoic acid. Metagenomic analysis of the cecum contents showed an upregulation in the abundance of Prevotella spp. in the experimental groups. However, we observed no significant differences in the abundances of other cecal microbes at phylum and genus levels. Furthermore, we observed significant associations between Prevotella spp. and the differentially abundant metabolites, such as cherubins, thiaburimamide, and 3,4-dihydroxy-L-phenylalanine, (r)-mevalonate, 5-O-methylalloptaeroxylin, nalidixic acid, and deoxyloganin (p < 0.05). Proteomic analysis revealed that the differentially expressed proteins (such as interferon-induced protein with tetratricopeptide repeats 5 (IFIT5), MHC-BF1, and death domain-associated protein (Daxx)) in the bone marrow of the chickens were primarily enriched in the immune network for IgA production and B cell receptor signaling pathway.

Conclusion

In conclusion, dietary ANE supplementation was found to enhance metabolic activity and energy utilization, improve growth performance, modulate cecal microbiota, and strengthen the immunity of Wenchang chickens.
Robustness of Single- and Dual-Energy Deep-Learning-Based Scatter Correction Models on Simulated and Real Chest X-rays
Authors: Freijo Clara , Herraiz Joaquin L. , Arias-Valcayo Fernando , Ibáñez Paula , Moreno Gabriela , Villa-Abaunza Amaia , Udías José Manuel
Published: December 2023

Chest X-rays (CXRs) represent the first tool globally employed to detect cardiopulmonary pathologies. These acquisitions are highly affected by scattered photons due to the large field of view required. Scatter in CXRs introduces background in the images, which reduces their contrast. We developed three deep-learning-based models to estimate and correct scatter contribution to CXRs. We used a Monte Carlo (MC) ray-tracing model to simulate CXRs from human models obtained from CT scans using different configurations (depending on the availability of dual-energy acquisitions). The simulated CXRs contained the separated contribution of direct and scattered X-rays in the detector. These simulated datasets were then used as the reference for the supervised training of several NNs. Three NN models (single and dual energy) were trained with the MultiResUNet architecture. The performance of the NN models was evaluated on CXRs obtained, with an MC code, from chest CT scans of patients affected by COVID-19. The results show that the NN models were able to estimate and correct the scatter contribution to CXRs with an error of <5%, being robust to variations in the simulation setup and improving contrast in soft tissue. The single-energy model was tested on real CXRs, providing robust estimations of the scatter-corrected CXRs.
Loss of Traditional Orchards and Its Impact on the Occurrence of Threatened Tree-Dwelling Bird Species
Authors: Kajtoch Łukasz
Published: December 2023

Horticulture is one of the land use types in agricultural landscapes, which is beneficial for nature if traditional ways of management are implemented. Orchards are affected by three negative transformations: abandonment that leads to afforestation; grubbing as a result of the cessation of fruit plantation; or intensification with the use of chemicals. In this study, changes in orchard management and structure were examined over a decade (2014–2023) in southern Poland (the Carpathians). Additionally, changes in the distribution of Syrian woodpeckers were assessed—a rare species of special concern in the European Union being a major nest hole excavator in orchards. Over a decade, trees in nearly one-fourth of orchards were removed, 15% of orchards were overgrown by forests due to abandonment, and only 40% remained unchanged. The changes were most pronounced in already abandoned orchards and many traditionally used ones. Fruit trees were grubbed in orchards in areas with a high density of people and roads, whereas succession prevailed in orchards in the vicinity of forests. During the same period, around 40% of woodpecker territories vanished, and this phenomenon was associated with tree grubbing or succession by forests. As the Syrian woodpecker requires protection in Europe, it is recommended to preserve traditional horticulture. Moreover, conservative cultivation of traditional varieties of fruit trees and agro-tourism in traditional orchards could be implemented in synergy with nature conservation.
Investigating the Profitability of Government-Funded Small-Scale Broiler Projects in Northern KwaZulu-Natal, South Africa
Authors: Mdletshe Sifiso Themba Clement , Obi Ajuruchukwu
Published: December 2023

The frequent failures of government-funded broiler enterprises raise concerns about the viability and wisdom of government funding for smallholders. This study therefore investigates the scope for the profitability of the small-scale broiler production and the range of socio-demographic and production issues that are implicated. The study area was the Northern KwaZulu-Natal (KZN) Province of South Africa, where smallholder broiler production is popular. A total of 75 randomly selected, small-scale broiler producers from three districts in Northern KZN, namely, King Cetshwayo, uMkhanyakude, and Zululand, participated in the survey out of the 134 small-scale broiler producers supported by the government. The analyses employed diverse descriptive analysis and included the calculation of the gross margin to proxy broiler chicken profitability. Three models were fitted for the empirical analysis, namely, the OLS, the Two-Stage Least Squares, and the Stochastic Frontier models, to determine the factors influencing profitability, correcting for endogeneity, and computing the technical efficiency and inefficiency of the small-scale broiler production system. The results show that the primary production and marketing challenges were the lack of infrastructure (abattoirs and refrigeration) and the lack of formal markets, including the lack of market information and high transport costs. On average, the sampled government-funded small-scale broiler enterprises achieved a positive gross profit margin of 31 percent, which is relatively low when compared to the small-scale farmers that work for a large-scale enterprise—the Commercial Chicken Farm, near Pietermaritzburg. It was revealed that the profits are significantly influenced by gender, farmgate price, access to market information, and access to extension services. The production system was also shown to be operating at a reasonably high technical efficiency, which is strongly influenced by flock size, feeds, and labour input, while age, gender, and educational level contributed to technical inefficiency. The recent crisis that was experienced by the poultry industry in South Africa linked to the outbreak of the Avian Flu and its devastating consequences point up the urgency for more investment in infrastructure to enhance bird safety at affordable costs. Although the government-funded small-scale broiler enterprises in Northern KZN were found to be viable, it is evident that they can be better, possibly through more capacity building and collective action to take full advantage of the economies of scale.
Validation of Criteria for Predicting Tractor Fuel Consumption and CO2 Emissions When Ploughing Fields of Different Shapes and Dimensions
Authors: Damanauskas Vidas , Janulevičius Algirdas
Published: December 2023

Climate change is linked to CO2 emissions, the reduction of which has become a top priority. In response to these circumstances, scientists must constantly develop new technologies that increase fuel efficiency and reduce emissions. Agriculture today is dominated by arable fields of various sizes, shapes, and dimensions, and to achieve fuel economy and environmental impact requirements, it is not enough to know only the principles of optimization of tillage processes; it is also necessary to understand the influence of field size and its shape and dimensions on tillage performance. The purpose of this research is to present a methodology that allows predicting tractor fuel demand and CO2 emissions per unit of ploughed area when ploughing field plots with different shapes and dimensions and to confirm a suitable variable for such a prediction. Theoretical calculations and experimental tests have shown that the field ploughing time efficiency coefficient is a useful metric for comparing field plots of different shapes and dimensions. This coefficient effectively describes tractor fuel consumption and CO2 emissions during ploughing operations on differently configured field plots. A reasonable method for calculating the real field ploughing time efficiency coefficient is based on field and tillage data and a practical determination method using tractor engine load reports. It was found that during the research, when ploughing six field plots of different shapes and dimensions, with an area of 6 ha, the field ploughing time efficiency coefficient varied from 0.68 to 0.82, and fuel consumption between 15.6 and 16.5 kg/ha. In the field plot of 6 ha, where the field ploughing time efficiency coefficient was 15% higher, the fuel consumption per unit area was lower by about 5.5%. The results of this study will help to effectively predict tillage time and tractor fuel consumption required for different field shapes and dimensions.
Intercropping Forage Sorghum with Sunnhemp at Different Seeding Rates to Improve Forage Production
Authors: Mosqueda Haley M. , Blaser Brock C. , O’Shaughnessy Susan A. , Rhoades Marty B.
Published: December 2023

Forage sorghum (Sorghum bicolor (L.) Moench) is well established in the Texas High Plains as a drought-tolerant forage that often requires additional feed to provide adequate protein for livestock. Intercropping sunnhemp (Crotalaria juncea L.), a legume, with forage sorghum, may increase crude protein. However, the optimal intercrop seeding ratio of sunnhemp to sorghum to improve crude protein content and maintain sufficient biomass is unknown. A two-year field experiment was conducted near Canyon, TX, USA, in 2020 and 2021 using sunnhemp intercropped at three seeding rates (16.8, 33.6, and 50.4 kg ha−1) with forage sorghum at four seeding rates (0, 2.8, 5.6, and 11.2 kg ha−1) under drip irrigation. This study was conducted to (1) evaluate growth potential for sunnhemp in a semiarid environment, (2) find a seeding ratio that can maintain forage sorghum dry matter production and improve forage quality, and (3) determine if a midseason harvest can be supported and further improve quality of the forage produced. Midseason, full-season, regrowth biomass, and forage quality were evaluated. Results indicated that a sunnhemp–forage-sorghum intercrop produced dry matter comparable to forage sorghum when sufficient heat units were obtained in the growing season. Forage with higher nutritive value was produced when the intercrop was harvested twice.
Ameliorating Forage Crop Resilience in Dry Steppe Zone Using Millet Growth Dynamics
Authors: Kurbanbayev Almas , Zargar Meisam , Yancheva Hristina , Stybayev Gani , Serekpayev Nurlan , Baitelenova Aliya , Mukhanov Nurbolat , Nogayev Adilbek , Akhylbekova Balzhan , Abdelkader Mostafa
Published: December 2023

Introducing new forage crops such as Japanese millet (Echinochloa frumentacea) and pearl millet (Pennisetum glaucum) is crucial for mitigating the impacts of climate change in the dry steppe zone, expanding forage crop options, and obtaining nutritious feed for the development of animal husbandry. The aim of this study was to assess the productivity and feed value of these crops. Field experiments were conducted in 2021 and 2022 to investigate the changes and variations in the yields and chemical compositions of Japanese millet and pearl millet when grown as sole crops or in mixed cropping in the dry steppe zone of northern Kazakhstan. Among the annual crops sown via sole cropping, the hay of the Japanese millet, sown in the third decade of May and harvested during full heading, was observed to have a higher content of raw protein and other nutrients than Sudanese grass hay, that is, the raw protein content was higher at 1.81%, the raw oil content at 0.12, and the raw ash content at 1.88%. In addition, among the mixtures of crops, the hay of the crop mixtures containing pearl millet, sown in the above period and harvested as hay during the milky stage, the full heading stage, and the formation of spikelets in the grain family stage, differed from the hay of Sudanese grass sown in the same period and collected during full heading in terms of the contents of raw protein and other nutrients and the low content of raw ash, that is, the content of raw protein was at 2.16%, raw oil at 0.39, raw ash at 0.95, without nitrogen extractives (WNEs) at 3.78, and starch at 0.11. The calcium content was higher by 0.08% and carotene by 0.11 mg/kg, and raw lentils were lower by 0.94%. The analysis of the results revealed that the variation in the crops’ phenological phases depended on the moisture availability and the sowing time.
Comparative Meropenem Pharmacodynamics and Emergence of Resistance against Carbapenem-Susceptible Non-Carbapenemase-Producing and Carbapenemase-Producing Enterobacterales: A Pharmacodynamic Study in a Hollow-Fiber Infection Model
Authors: Golikova Maria V. , Alieva Kamilla N. , Strukova Elena N. , Kondratieva Daria A. , Petrova Nika F. , Petrova Mayya A. , Zinner Stephen H.
Published: December 2023

Resistance to carbapenems has become a problem due to Klebsiella pneumoniae (K. pneumoniae), harboring carbapenemases. Among them, there are isolates that are recognized as carbapenem-susceptible; however, these carbapenemase-producing strains with low meropenem minimal inhibitory concentrations (MICs) may pose a threat to public health. We aimed to investigate the impact of the ability to produce carbapenemases by a bacterial isolate on the effectiveness of meropenem in the hollow-fiber infection model. K. pneumoniae and Escherichia coli (E. coli) strains with equal meropenem MICs but differing in their ability to produce carbapenemases were used in pharmacodynamic simulations with meropenem. In addition to standard MIC determination, we assessed the MICs against tested strains at high inoculum density to test if the inoculum effect occurs. According to pharmacodynamic data, the carbapenemase-producing strains were characterized with a relatively decreased meropenem effectiveness compared to non-producers. Meanwhile, the effect of meropenem perfectly correlated with the meropenem exposure expressed as the DOSE/MIC ratio when high-inoculum (HI) MICs but not standard-inoculum (SI) MICs were used for regression analysis. It could be concluded that meropenem-susceptible carbapenemase-producing strains may not respond to meropenem therapy; the antibiotic inoculum effect (IE) may have a prognostic value to reveal the meropenem-susceptible Enterobacterales that harbor carbapenemase genes.
Development of Pharmacological Strategies with Therapeutic Potential in Ischemic Stroke
Authors: Escobar-Peso Alejandro , Martínez-Alonso Emma , Masjuan Jaime , Alcázar Alberto
Published: December 2023

Acute ischemic stroke constitutes a health challenge with great social impact due to its high incidence, with the social dependency that it generates being an important source of inequality. The lack of treatments serving as effective neuroprotective therapies beyond thrombolysis and thrombectomy is presented as a need. With this goal in mind, our research group’s collaborative studies into cerebral ischemia and subsequent reperfusion concluded that there is a need to develop compounds with antioxidant and radical scavenger features. In this review, we summarize the path taken toward the identification of lead compounds as potential candidates for the treatment of acute ischemic stroke. Evaluations of the antioxidant capacity, neuroprotection of primary neuronal cultures and in vivo experimental models of cerebral ischemia, including neurological deficit score assessments, are conducted to characterize the biological efficacy of the various neuroprotective compounds developed. Moreover, the initial results in preclinical development, including dose–response studies, the therapeutic window, the long-term neuroprotective effect and in vivo antioxidant evaluation, are reported. The results prompt these compounds for clinical trials and are encouraging regarding new drug developments aimed at a successful therapy for ischemic stroke.
Exploring the Chemical Composition of Female Zucchini Flowers for Their Possible Use as Nutraceutical Ingredient
Authors: Castaldo Luigi , Lombardi Sonia , Izzo Luana , Ritieni Alberto
Published: December 2023

The zucchini (Cucurbita pepo L.) plant is well known for its fruits; however, its edible flowers appear to contain several active molecules, including polyphenols, which display poor bioaccessibility after gastrointestinal digestion (GiD). This study explores the bioaccessibility of polyphenols and antioxidant capacity within zucchini flower extracts during simulated GiD. Two nutraceutical formulations, non-acid-resistant (NAcR) and acid-resistant (AcR) capsules containing an aqueous extract of zucchini flowers, were employed in this investigation. Additionally, high-resolution mass spectrometry (Q-Orbitrap HRMS) was utilized for a comprehensive analysis of their polyphenolic constituents. Predominantly, rutin and isorhamnetin-3-rutinoside were the most prevalent compounds detected in the samples (514.62 and 318.59 mg/kg, respectively). Following in vitro GiD, the extract encapsulated in AcR capsules exhibited enhanced bioaccessibility during both the duodenal (189.2 and 162.5 mg GAE/100 g, respectively) and colonic stages (477.4 and 344.7 mg GAE/100 g, respectively) when compared with the extract encapsulated in NAcR capsules. This suggests that gastric acidity adversely impacted the release of polyphenols from NAcR capsules. In conclusion, the aqueous zucchini flower extract emerges as a promising and readily accessible source of dietary polyphenols. Moreover, the utilization of AcR capsules presents a potential nutraceutical formulation strategy to improve polyphenol bioaccessibility, enhancing its applicability in promoting health and well-being.
Optical Pupil Shift Correction Method for Large Ground-Based Optical Telescopes
Authors: Wang Honghao , Wang Jianli , Cao Yuyan , Chen Tao , Li Hongwen
Published: December 2023

Cassegrain telescopes with larger apertures suffer significant optical pupil shifting errors caused by gravity and temperature gradients. This study constructs an optical model of a large Cassegrain telescope and a method to calculate and compensate its shifting error. First, the optical structure is simplified by incorporating only the most relevant telescope components, and the system optics is modeled using ray tracing. A computer-aided mounting-based method for calculating the misalignment error of primary and secondary mirrors is proposed, with the spatial position change of secondary mirrors as the input. Next, a compensation method based on the coma-free point theory of the Cassegrain system is proposed, with the main mirror’s optical axis as the reference. Finally, using a 4 m aperture telescope as an example, the gravity- and thermal-deformation-induced shifting error is simulated. Based on this simulation, the secondary mirror position is adjusted using a secondary mirror-adjustment mechanism Hexapod platform to compensate for the misalignment error. Both gravity and temperature are major causes of shift in the pupil position, with a maximum shift of 18 mm. After compensation, this shift is controlled within 1 mm. The modeling method and the simulation process mentioned in this research can also be used in the other relevant fields.