bluuebunny/crossref_metadata_2025 huggingface dataset:
Z3495, a LysR-Type Transcriptional Regulator Encoded in O Island 97, Regulates Virulence Gene Expression in Enterohemorrhagic Escherichia coli O157:H7
Authors: Wang Qian , Wei Yi , Huang Yu , Qin Jingliang , Liu Bin , Liu Ruiying , Chen Xintong , Li Dan , Wang Qiushi , Li Xiaoya , Yang Xinyuan , Li Yuanke , Sun Hao
Published: January 2024

Enterohemorrhagic Escherichia coli (EHEC) is an important foodborne pathogen that infects humans by colonizing the large intestine. The genome of EHEC O157:H7 contains 177 unique O islands (OIs). Certain OIs significantly contribute to the heightened virulence and pathogenicity exhibited by EHEC O157:H7. However, the function of most OI genes remains unknown. We demonstrated here that EHEC O157:H7 adherence to and colonization of the mouse large intestine are both dependent on OI-97. Z3495, which is annotated as a LysR-type transcriptional regulator and encoded in OI-97, contributes to this phenotype. Z3495 activated the locus of enterocyte effacement (LEE) gene expression, promoting bacterial adherence. Deletion of z3495 significantly decreased the transcription of ler and other LEE genes, the ability to adhere to the host cells, and colonization in the mouse large intestine. Furthermore, the ChIP-seq results confirmed that Z3495 can directly bind to the promoter region of rcsF, which is a well-known activator of Ler, and increase LEE gene expression. Finally, phylogenetic analysis revealed that Z3495 is a widespread transcriptional regulator in enterohemorrhagic and enteropathogenic Escherichia coli. As a result of this study, we have gained a deeper understanding of how bacteria control their virulence and provide another example of a laterally acquired regulator that regulates LEE gene expression in bacteria.
Mineralogy of the Steel Mountain Anorthosite Complex, Western Newfoundland Appalachians, Canada: Petrogenesis and Tectonic Affinity
Authors: Pe-Piper Georgia , Piper David J. W. , Dessureau Gilles
Published: January 2024

The Steel Mountain complex in western Newfoundland is one of several possible peri-Gondwanan basement slivers that have been emplaced along the northwestern margin of the Appalachian Orogen. The complex includes feldspathic anorthosite, gabbronorite, and magnetite-ilmenite ore. Megacrysts of plagioclase (andesine), orthopyroxene (hypersthene) and amphibole (hornblende) were analyzed by electron microprobe and they are of similar composition to the groundmass minerals. The ferromagnesian megacrysts have exsolution lamellae of Fe-Ti oxides, magnesio–hornblende, and clinopyroxene. The hornblende megacrysts and the lack of plagioclase in the exsolution lamellae are unusual for Proterozoic anorthosites and result from unusual abundance of water in the parental magma. Whole-rock Nd-Sm isotope determinations give an isochron age of ~1.2 Ga. Ar-Ar dating of biotite rims on hornblende shows an early Ordovician plateau age and the final heating step suggests a ~0.8 Ga age for the amphibole itself. Taken together with published zircon geochronology, these data suggest a provenance in the southern peri-Gondwanan Appalachians.
Genesis of Caoziwa Pb–Zn Deposit in Tengchong Block, SW China: Constraints from Sulfur Isotopic and Trace Elemental Compositions of Sulfides
Authors: Cheng Yan , Yang Chunhai , Deng Mingguo , Bai Fuxiang , Chen Fuchuan
Published: January 2024

The Caoziwa Pb–Zn deposit is one of the typical vein-type Pb–Zn deposits in the western part of the Tengchong block. Due to limited research, the genesis of these deposits is unknown. In this study, the sulfur isotopic and trace elemental compositions of sulfides from the Caoziwa Pb–Zn deposit were analyzed to trace the sources of ore-forming materials, and to reveal the genetic type of this deposit. The results show that abundant Co, Ni, As, and Se, and less Cu, Zn, Ag, Cd, Sn, Sb, Te, Pb, and Bi could enter pyrite by isomorphic substitution. Elemental Mn, Fe, Cd, Co, and Ni could substitute Zn to enter sphalerite, while the contents of Ag, Sn, and Sb are mainly controlled by the Pb-rich inclusions in sphalerite. Elemental Bi, Sb, Cd, Sn, Ag, and Tl mainly enter the galena grains via an isomorphic substitution mechanism of (Bi, Sb)3+ + (Cd, Sn)2+ + (Ag, Tl)+ ↔ 2Pb2+. Both sulfur isotopic compositions and trace elemental compositions indicate that the ore-forming materials and fluids of the Caoziwa Pb–Zn deposit mainly originate from magmatic hydrothermal fluid related to Paleocene granitic magmatism. Combined with the geological facts that some skarnizations developed in the northern part of the ore field near the Paleocene granite, the Caoziwa Pb–Zn deposit is suggested to be a magmatic hydrothermal vein-type deposit that probably belongs to a distal part of a skarn mineralization system developed by the intrusion of Paleocene granitic magmatism in the western part of the Tengchong block.
Early Triassic Episode of the Kresty Volcano–Plutonic Complex Formation in the Maymecha-Kotuy Alkaline Province, Polar Siberia: Geochemistry, Petrology and Uranium–Lead Geochronology
Authors: Sazonov Anatoly M. , Gertner Igor F. , Mustafaev Agababa A. , Krasnova Tatyana S. , Kolmakov Yurii V. , Kingsbury Cole G. , Gogoleva Vera A.
Published: January 2024

The Kresty volcano–plutonic complex (KVPC) is one of the representatives of the alkaline–ultrabasic magmatism in the Maymecha-Kotuy Alkaline Province in Polar Siberia. The geological structure of the KVPC consists of intrusive formations of olivinite–pyroxenite and melilitolite–monticellitolite bodies, a series of rocks that break through dikes of trachydolerites, syenites, granosyenites, alkaline picrites and lamprophyres. This paper summarizes the results of the authors’ long-term research on the geological structure and features of the material composition of the intrusive magmatic rocks, including geochemistry, mineralogy, distribution of rare earth elements (REE), as well as the results of isotope studies. The multielement composition of the KVPC intrusions demonstrates a complex geodynamic paleoenvironment of the formation as plume nature with signs of subduction and collision. For the ultrabasic series with normal alkalinity from the first phase of the KVPC, a Sm-Nd isochron age yielded an Early Triassic (T1) result of 251 ± 25 Ma. Here, we present U-Pb dating of zircons and perovskite of high-calcium intrusive formations and a dyke complex of alkaline syenites. Thus, for the intrusion of kugdite (according to perovskite), the age determination was 249 ± 4 Ma, and for the crosscutting KVPC dykes of syenites (according to zircon) 249 ± 1 Ma and 252 ± 1 Ma. The age of the most recent dike is almost identical to the age of the main intrusive phases of the KVPC (T1), which corresponds to a larger regional event of the Siberian LIP—251 Ma. According to isotopic Sr-Nd parameters, the main source of KVPC magmas is a PREMA-type material. For dyke varieties, we assume there was an interaction of plume melts with the continental crust. The new age results obtained allow us to further constrain the episodes of alkaline–ultrabasic intrusions in Polar Siberia, taking into account the interaction of mantle plume matter and crustal material.
Origin of Zn-Pb Mineralization of the Vein Bt23C, Bytíz Deposit, Příbram Uranium and Base-Metal Ore District, Czech Republic: Constraints from Occurrence of Immiscible Aqueous–Carbonic Fluids
Authors: Ulmanová Jana , Dolníček Zdeněk , Škácha Pavel , Sejkora Jiří
Published: January 2024

The mineralogical, fluid inclusion, and stable isotope (C, O) study was conducted on a Late Variscan Zn-Pb vein Bt23C, Příbram uranium and base-metal district, Bohemian Massif, Czech Republic. The vein is hosted by folded Proterozoic clastic sediments in exo-contact of a Devonian-to-Lower-Carboniferous granitic pluton. Siderite, dolomite-ankerite, calcite, quartz, baryte, galena, sphalerite, V-rich mica (roscoelite to an unnamed V-analogue of illite), and chlorite (chamosite) form the studied vein samples. The banded texture of the vein was modified by the episodic dissolution of earlier carbonates and/or sphalerite. Petrographic, microthermometric, and Raman studies of fluid inclusions proved a complicated fluid evolution, related to the activity of aqueous fluids and to an episode involving an aqueous–carbonic fluid mixture. Homogenization temperatures of aqueous inclusions decreased from ~210 to ~50 °C during the evolution of the vein, and salinity varied significantly from pure water up to 27 wt.% NaCl eq. The aqueous–carbonic fluid inclusions hosted by late quartz show highly variable phase compositions caused by the entrapment of accidental mixtures of a carbonic and an aqueous phase. Carbonic fluid is dominated by CO2 with minor CH4 and N2, and the associated aqueous solution has a medium salinity (6–14 wt.% NaCl eq.). The low calculated fluid δ18O values (−4.7 to +3.6‰ V-SMOW) suggest a predominance of surface waters during the crystallization of dolomite-ankerite and calcite, combined with a well-mixed source of carbon with δ13C values ranging between −8.2 and −10.5‰ V-PDB. The participation of three fluid endmembers is probable: (i) early high-temperature high-salinity Na>Ca-Cl fluids from an unspecified “deep” source; (ii) late low-salinity low-temperature waters, likely infiltrating from overlying Permian freshwater partly evaporated piedmont basins; (iii) late high-salinity chloridic solutions with both high and low Ca/Na ratios, which can represent externally derived marine brines, and/or local shield brines. The source of volatiles can be (i) in deep crust, (ii) from interactions of fluids with sedimentary wall rocks and/or (iii) in overlying Permian piedmont basins containing, in places, coal seams. The event dealing with heterogeneous CO2-bearing fluids yielded constraints on pressure conditions of ore formation (100–270 bar) as well as on the clarification of some additional genetic aspects of the Příbram’s ores, including the reasons for the widespread dissolution of older vein fill, the possible re-cycling of some ore-forming components, pH changes, and occasionally observed carbon isotope shift due to CO2 degassing.
The Application of Box–Behnken Design for Investigating the Supercritical CO2 Foaming Process: A Case Study of Thermoplastic Polyurethane 85A
Authors: Khudaida Salal Hasan , Yen Shih-Kuo , Su Chie-Shaan
Published: January 2024

Thermoplastic polyurethane (TPU) is a versatile polymer with unique characteristics such as flexibility, rigidity, elasticity, and adjustable properties by controlling its soft and hard segments. To properly design and understand the TPU foaming process through supercritical CO2, a design of experiments approach, the Box–Behnken design (BBD) was adopted using commercial TPU 85A as the model compound. The effect of saturation pressure, saturation temperature, and immersion time on the mean pore size and expansion ratio were investigated. The design space for the production of TPU foam was shown, and the significance of process parameters was confirmed using the analysis of variance (ANOVA). In addition, extrapolation foaming experiments were designed and validated the feasibility of the response surface model developed via BBD. It was found that the pore size of TPU 85A foam could be controlled within 13 to 60 μm, and a stable expansion ratio could be designed up to six.
Experimental and DFT Study of Monensinate and Salinomycinate Complexes Containing {Fe3(µ3–O)}7+ Core
Authors: Petkov Nikolay , Tadjer Alia , Encheva Elzhana , Cherkezova-Zheleva Zara , Paneva Daniela , Stoyanova Radostina , Kukeva Rositsa , Dorkov Petar , Pantcheva Ivayla
Published: January 2024

Two trinuclear oxo-centred iron(III) coordination compounds of monensic and salinomycinic acids (HL) were synthesized and their spectral properties were studied using physicochemical/thermal methods (FT–IR, TG–DTA, TG–MS, EPR, Mössbauer spectroscopy, powder XRD) and elemental analysis. The data suggested the formation of [Fe3(µ3–O)L3(OH)4] and the probable complex structures were modelled using the DFT method. The computed spectral parameters of the optimized constructs were compared to the experimentally measured ones. In each complex, three metal centres were joined together at the axial position by a μ3–O unit to form a {Fe3O}7+ core. The antibiotics monoanions served as bidentate ligands through the carboxylate and hydroxyl groups located at the termini. The carboxylate moieties played a dual role bridging each two metal centres. Hydroxide anions secured the overall neutral character of the coordination species. Mössbauer spectra displayed asymmetric quadrupole doublets that were consistent with the existence of two types of high-spin iron(III) sites with different environments—two Fe[O5] and one Fe[O6] centres. The solid-state EPR studies confirmed the +3 oxidation state of iron with a total spin St = 5/2 per trinuclear cluster. The studied complexes are the first iron(III) coordination compounds of monensin and salinomycin reported so far.
Dual/Multi-Modal Image-Guided Diagnosis and Therapy Based on Luminogens with Aggregation-Induced Emission
Authors: Zhu Linlin , Wu Wenbo
Published: January 2024

The combination of multiple imaging methods has made an indelible contribution to the diagnosis, surgical navigation, treatment, and prognostic evaluation of various diseases. Due to the unique advantages of luminogens with aggregation-induced emission (AIE), their progress has been significant in the field of organic fluorescent contrast agents. Herein, this manuscript summarizes the recent advancements in AIE molecules as contrast agents for optical image-based dual/multi-modal imaging. We particularly focus on the exceptional properties of each material and the corresponding application in the diagnosis and treatment of diseases.
Research Progress of Non-Noble Metal Catalysts for Carbon Dioxide Methanation
Authors: Cui Yingchao , He Shunyu , Yang Jun , Gao Ruxing , Hu Kehao , Chen Xixi , Xu Lujing , Deng Chao , Lin Congji , Peng Shuai , Zhang Chundong
Published: January 2024

The extensive utilization of fossil fuels has led to a rapid increase in atmospheric CO2 concentration, resulting in various environmental issues. To reduce reliance on fossil fuels and mitigate CO2 emissions, it is important to explore alternative methods of utilizing CO2 and H2 as raw materials to obtain high-value-added chemicals or fuels. One such method is CO2 methanation, which converts CO2 and H2 into methane (CH4), a valuable fuel and raw material for other chemicals. However, CO2 methanation faces challenges in terms of kinetics and thermodynamics. The reaction rate, CO2 conversion, and CH4 yield need to be improved to make the process more efficient. To overcome these challenges, the development of suitable catalysts is essential. Non-noble metal catalysts have gained significant attention due to their high catalytic activity and relatively low cost. In this paper, the thermodynamics and kinetics of the CO2 methanation reaction are discussed. The focus is primarily on reviewing Ni-based, Co-based, and other commonly used catalysts such as Fe-based. The effects of catalyst supports, preparation methods, and promoters on the catalytic performance of the methanation reaction are highlighted. Additionally, the paper summarizes the impact of reaction conditions such as temperature, pressure, space velocity, and H2/CO2 ratio on the catalyst performance. The mechanism of CO2 methanation is also summarized to provide a comprehensive understanding of the process. The objective of this paper is to deepen the understanding of non-noble metal catalysts in CO2 methanation reactions and provide insights for improving catalyst performance. By addressing the limitations of CO2 methanation and exploring the factors influencing catalyst effectiveness, researchers can develop more efficient and cost-effective catalysts for this reaction.
Improving the Diagnosis of Dermatitis Herpetiformis Using the Intraepithelial Lymphogram
Authors: Fernández-Bañares Fernando , Crespo Laura , Planella Montserrat , Farrais Sergio , Izquierdo Sandra , López-Palacios Natalia , Roy Garbiñe , Vidal Judith , Núñez Concepción
Published: January 2024

Dermatitis herpetiformis is a cutaneous manifestation of celiac disease. Phenotyping of intraepithelial lymphocytes in the small bowel mucosa can strengthen the diagnosis of celiac disease when it is not clear-cut. We aim to evaluate the usefulness of the intraepithelial lymphogram to confirm dermatitis herpetiformis in equivocal cases. We performed a retrospective multicenter study on patients diagnosed with dermatitis herpetiformis and collected data from the intraepithelial lymphogram assessed by flow cytometry. A total of 36 patients were analyzed in relation to the severity of intestinal damage (18 had non-atrophic mucosa) at baseline (N = 28) and/or after the adoption of a gluten-free diet (median follow-up of three years, N = 16). We observed that patients with atrophy more often had positive celiac serology (p = 0.019), celiac clinical symptoms (p = 0.018), and iron-deficiency anemia (p = 0.018), but the severity of skin damage was similar in both groups (p = 0.79). At baseline, increased TCRγδ+ cells were present in 94% of patients with atrophy and 67% with non-atrophic lesions (p = 0.13). After a gluten-free diet, increased TCRγδ+ cells persisted in 100% and 63% of cases, respectively (p = 0.21). We concluded that increased TCRγδ+ cells may be helpful in confirming the diagnosis of dermatitis herpetiformis in equivocal cases, even in patients who were started on a gluten-free diet.
Frequency and Overlap of Cachexia, Malnutrition, and Sarcopenia in Elderly Patients with Diabetes Mellitus: A Study Using AWGC, GLIM, and AWGS2019
Authors: Ida Satoshi , Imataka Kanako , Morii Shoki , Katsuki Keitaro , Murata Kazuya
Published: January 2024

This study aimed to estimate the frequency and overlap of cachexia, malnutrition, and sarcopenia in elderly patients with diabetes mellitus. Patients who were aged at least 65 years, had diabetes mellitus, and were regularly visiting the Ise Red Cross Hospital on an outpatient basis were included. The patients were assessed to determine whether they had cachexia, malnutrition, and sarcopenia according to the Asian Working Group for Cachexia criteria, the Global Leadership Initiative on Malnutrition criteria, and the Asian Working Group for Sarcopenia 2019 criteria. A total of 510 patients (310 men and 200 women) were analyzed in this study. Sarcopenia, cachexia, and malnutrition were found in 84 patients (16.4%), 40 patients (7.8%) (17.8% among patients with chronic diseases), and 110 patients (21.5%), respectively. Among patients with sarcopenia, the frequencies of cachexia and malnutrition were 30% and 71.4%, respectively. Among patients with cachexia, the frequencies of sarcopenia and malnutrition were 65% and 90%, respectively, and among those with malnutrition, the frequencies of sarcopenia and cachexia were 54% and 32.7%, respectively. The overlap among cachexia, malnutrition, and sarcopenia appears to be an important factor to be considered in the treatment of elderly patients with diabetes mellitus.
From Stress Tolerance to Virulence: Recognizing the Roles of Csps in Pathogenicity and Food Contamination
Authors: Cardoza Evieann , Singh Harinder
Published: January 2024

Be it for lab studies or real-life situations, bacteria are constantly exposed to a myriad of physical or chemical stresses that selectively allow the tolerant to survive and thrive. In response to environmental fluctuations, the expression of cold shock domain family proteins (Csps) significantly increases to counteract and help cells deal with the harmful effects of stresses. Csps are, therefore, considered stress adaptation proteins. The primary functions of Csps include chaperoning nucleic acids and regulating global gene expression. In this review, we focus on the phenotypic effects of Csps in pathogenic bacteria and explore their involvement in bacterial pathogenesis. Current studies of csp deletions among pathogenic strains indicate their involvement in motility, host invasion and stress tolerance, proliferation, cell adhesion, and biofilm formation. Through their RNA chaperone activity, Csps regulate virulence-associated genes and thereby contribute to bacterial pathogenicity. Additionally, we outline their involvement in food contamination and discuss how foodborne pathogens utilize the stress tolerance roles of Csps against preservation and sanitation strategies. Furthermore, we highlight how Csps positively and negatively impact pathogens and the host. Overall, Csps are involved in regulatory networks that influence the expression of genes central to stress tolerance and virulence.
The Study on Timolol and Its Potential Phototoxicity Using Chemical, In Silico and In Vitro Methods
Authors: Lejwoda Karolina , Gumieniczek Anna , Filip Agata , Naumczuk Beata
Published: January 2024

Timolol (TIM) is a non-selective ß-adrenergic receptor antagonist used orally for the treatment of hypertension and heart attacks, and topically for treating glaucoma; lately, it has also been used in some specific dermatological problems. In the present study, its photodegradation and potential risk of phototoxicity were examined using chemical, in silico and in vitro methods. The UV/VIS irradiated solutions of TIM at pH 1–13 were subjected to LC-UV and UPLC-HRMS/MS analyses showing pseudo first-order kinetics of degradation and several degradation products. The structures of these photodegradants were elucidated by fragmentation path analysis based on high resolution (HR) fragmentation mass spectra, and then used for toxicity evaluation using OSIRIS Property Explorer and Toxtree. Potential risk of phototoxicity was also studied using chemical tests for detecting ROS under UV/VIS irradiation and in vitro tests on BALB/c 3T3 mouse fibroblasts (MTT, NRU and Live/Dead tests). TIM was shown to be potentially phototoxic because of its UV/VIS absorptive properties and generation ROS during irradiation. As was observed in the MTT and NRU tests, the co-treatment of fibroblasts with TIM and UV/VIS light inhibited cell viability, especially when concentrations of the drug were higher than 50 µg/mL.
Caregivers and Family Members’ Vulnerability in End-of-Life Decision-Making: An Assessment of How Vulnerability Shapes Clinical Choices and the Contribution of Clinical Ethics Consultation
Authors: Nicoli Federico , Grossi Alessandra Agnese , Picozzi Mario
Published: January 2024

Patient-and-family-centered care (PFCC) is critical in end-of-life (EOL) settings. PFCC serves to develop and implement patient care plans within the context of unique family situations. Key components of PFCC include collaboration and communication among patients, family members and healthcare professionals (HCP). Ethical challenges arise when the burdens (e.g., economic, psychosocial, physical) of family members and significant others do not align with patients’ wishes. This study aims to describe the concept of vulnerability and the ethical challenges faced by HCPs in these circumstances. Further, it assesses the contribution of clinical ethics consultation (CEC) in assisting HCPs to face these difficult ethical conundrums. Two clinical cases are analyzed using the Circle Method of CEC. The first regards the difficulty faced by the doctor in justifying treatments previously agreed upon between the patient and his/her friends. The second regards the patient’s concern about being a burden on their family. Family burdens in EOL settings challenge PFCC in that patient autonomy may be disregarded. This compromises shared decision-making between the patient, family and HCPs as a core component of PFCC. In their ability to promote a collaborative approach, CECs may assist in the successful implementation of PFCC.
Species Survey of Leaf Hyponasty Responses to Warming Plus Elevated CO2
Authors: Thomas Michael D. , Roberts Reagan , Heckathorn Scott A. , Boldt Jennifer K.
Published: January 2024

Atmospheric carbon dioxide (CO2) concentrations are increasing and may exceed 800 ppm by 2100. This is increasing global mean temperatures and the frequency and severity of heatwaves. Recently, we showed for the first time that the combination of short-term warming and elevated carbon dioxide (eCO2) caused extreme upward bending (i.e., hyponasty) of leaflets and leaf stems (petioles) in tomato (Solanum lycopersicum), which reduced growth. Here, we examined additional species to test the hypotheses that warming + eCO2-induced hyponasty is restricted to compound-leaved species, and/or limited to the Solanaceae. A 2 × 2 factorial experiment with two temperatures, near-optimal and supra-optimal, and two CO2 concentrations, ambient and elevated (400, 800 ppm), was imposed on similarly aged plants for 7–10 days, after which final petiole angles were measured. Within Solanaceae, compound-leaf, but not simple-leaf, species displayed increased hyponasty with the combination of warming + eCO2 relative to warming or eCO2 alone. In non-solanaceous species, hyponasty, leaf-cupping, and changes in leaf pigmentation as a result of warming + eCO2 were variable across species.
Enhancing Road Extraction in Large-Scale Complex Terrain through Multi-Source Remote Sensing Image Fusion and Optimization
Authors: Fu Longqiang , Chai Huiming , Lv Xiaolei
Published: January 2024

In large-scale remote sensing scenarios characterized by intricate terrain, the straightforward road imaging features in synthetic aperture radar (SAR) images make them susceptible to interference from other elements such as ridges, compromising the robustness of conventional SAR image road extraction methods. This paper introduces a method that integrates Gaofen-3 (GF-3) with a resolution of 3.0 m, Digital Elevation Models (DEMs), and Gaofen-2 (GF-2) remote sensing image data with a resolution of 4.0 m, aiming to improve the performance of road extraction in complex terrain. Leveraging DEMs, this study addresses the limitations in feature-based SAR algorithms, extending their application to complex remote sensing scenarios. Decision-level fusion, integrating SAR and multispectral images, further refines road extraction precision. To overcome issues related to terrain interference, including fragmented road segments, an adaptive rotated median filter and graph-theory-based optimization are introduced. These advancements collectively enhance road recognition accuracy and topological precision. The experimental results validate the effectiveness of the multi-source remote sensing image fusion and optimization methods. Compared to road extraction from multispectral images, the F1-score of the proposed method on the test images increased by 2.18%, 4.22%, and 1.4%, respectively.
Examining the Dynamics of Vegetation in South Korea: An Integrated Analysis Using Remote Sensing and In Situ Data
Authors: Pradhan Biswajeet , Yoon Sungsoo , Lee Sanghun
Published: January 2024

Vegetation constitutes the primary component of terrestrial ecosystems and plays a crucial role in examining global climate change and its impacts. Assessing vegetation dynamics over significant periods of time can provide critical information on changes in vegetation cover and regional climate. Satellite-based remote sensing products offer a unique opportunity to analyze these changes at a country scale. South Korea has a unique history of forest regeneration, and studying this relationship provides an opportunity to examine its impact. To achieve this, we employ the vegetation–precipitation relationship using time series segmentation on residual trends (TSS-RESTREND), a statistical method that calculates the correlation between the annual maximum vegetation index and cumulative rainfall. This method helps identify time-step changes related to structural changes in the ecosystem. Using the enhanced vegetation index (EVI) and rainfall, this study aims to uncover the underlying dynamics and driving forces behind observed ecosystem changes, facilitating a deeper comprehension of the intricate interactions between vegetation and precipitation through the application of TSS-RESTREND. The findings increase knowledge of the complex relationship between vegetation growth and rainfall, given the country’s commendable forest regeneration program, even in the face of rapid urbanization. Moreover, the integration of finer resolution land cover images derived from in situ data not only provides a nuanced understanding of vegetation dynamics across diverse regions, but also underscores the critical need for continued collection of in situ data to provide more accurate and comprehensive analyses. The findings underline the pivotal role played by land resource management practices and underscores the significance of implementing adaptive conservation strategies, thereby contributing to the preservation of a stable and sustainable vegetation cover. The findings serve as a call for the ecosystem monitoring and strategic adaptation required to examine the environmental and ecosystem structural changes in the region. This study sheds light on the critical relationship between land management practices, environmental sustainability, and the resilience of the region’s vegetation amidst an ever-changing landscape.
Toward Precision Agriculture in Outdoor Vertical Greenery Systems (VGS): Monitoring and Early Detection of Stress Events
Authors: Zuckerman Noa , Cohen Yafit , Alchanatis Victor , Lensky Itamar M.
Published: January 2024

Vertical greenery systems (VGS) have been proposed as a nature-based solution to mitigate the adverse effects of urban heat islands and climate change in cities. However, large-scale VGS are costly and require ongoing maintenance, typically carried out manually through trial and error based on professional experience. Advanced management is essential for the sustainability of VGS due to its limited accessibility and associated costs. To address these challenges, we examined the use of remote sensing methods for outdoor VGS monitoring as a basis for a precision agriculture approach for VGS management and maintenance. This study presents the first ongoing monitoring of real-scale VGS using thermal, hyperspectral, and RGB vegetation indices. These indices were employed for the early detection of vegetation stress, focusing on two case studies exhibiting visible yellowing symptoms. Through the application of unsupervised classification techniques, stressed pixels were successfully detected 14–35 days before visual yellowing, achieving an accuracy of 0.85–0.91. Additionally, the thermal index provided valuable information regarding the spatial distribution of watering along the VGS. Stress maps based on noninvasive methods were demonstrated, forming the basis of a spatial decision support system capable of detecting issues related to plant vitality and VGS irrigation management.
Inter-Domain Invariant Cross-Domain Object Detection Using Style and Content Disentanglement for In-Vehicle Images
Authors: Jiang Zhipeng , Zhang Yongsheng , Wang Ziquan , Yu Ying , Zhang Zhenchao , Zhang Mengwei , Zhang Lei , Cheng Binbin
Published: January 2024

The accurate detection of relevant vehicles, pedestrians, and other targets on the road plays a crucial role in ensuring the safety of autonomous driving. In recent years, object detectors based on Transformers or CNNs have achieved excellent performance in the fully supervised paradigm. However, when the trained model is directly applied to unfamiliar scenes where the training data and testing data have different distributions statistically, the model’s performance may decrease dramatically. To address this issue, unsupervised domain adaptive object detection methods have been proposed. However, these methods often exhibit decreasing performance when the gap between the source and target domains increases. Previous works mainly focused on utilizing the style gap to reduce the domain gap while ignoring the content gap. To tackle this challenge, we introduce a novel method called IDI-SCD that effectively addresses both the style and content gaps simultaneously. Firstly, the domain gap is reduced by disentangling it into the style gap and content gap, generating corresponding intermediate domains in the meanwhile. Secondly, during training, we focus on one single domain gap at a time to achieve inter-domain invariance. That is, the content gap is tackled while maintaining the style gap, and vice versa. In addition, the style-invariant loss is used to narrow down the style gap, and the mean teacher self-training framework is used to narrow down the content gap. Finally, we introduce a multiscale fusion strategy to enhance the quality of pseudo-labels, which mainly focus on enhancing the detection performance for extreme-scale objects (very large or very small objects). We conduct extensive experiments on four mainstream datasets of in-vehicle images. The experimental results demonstrate the effectiveness of our method and its superiority over most of the existing methods.
SatellStitch: Satellite Imagery-Assisted UAV Image Seamless Stitching for Emergency Response without GCP and GNSS
Authors: Wei Zijun , Lan Chaozhen , Xu Qing , Wang Longhao , Gao Tian , Yao Fushan , Hou Huitai
Published: January 2024

Rapidly stitching unmanned aerial vehicle (UAV) imagery to produce high-resolution fast-stitch maps is key to UAV emergency mapping. However, common problems such as gaps and ghosting in image stitching remain challenging and directly affect the visual interpretation value of the imagery product. Inspired by the data characteristics of high-precision satellite images with rich access and geographic coordinates, a seamless stitching method is proposed for emergency response without the support of ground control points (CGPs) and global navigation satellite systems (GNSS). This method aims to eliminate stitching traces and solve the problem of stitching error accumulation. Firstly, satellite images are introduced to support image alignment and geographic coordinate acquisition simultaneously using matching relationships. Then a dynamic contour point set is constructed to locate the stitching region and adaptively extract the fused region of interest (FROI). Finally, the gradient weight cost map of the FROI image is computed and the Laplacian pyramid fusion rule is improved to achieve seamless production of the fast-stitch image map with geolocation information. Experimental results indicate that the method is well adapted to two representative sets of UAV images. Compared with the Laplacian pyramid fusion algorithm, the peak signal-to-noise ratio (PSNR) of the image stitching results can be improved by 31.73% on average, and the mutual information (MI) can be improved by 19.98% on average. With no reliance on CGPs or GNSS support, fast-stitch image maps are more robust in harsh environments, making them ideal for emergency mapping and security applications.
Effect of Different Anthelmintic Drugs on the Development and Efficacy of Duddingtonia flagrans
Authors: Zegbi Sara , Sagües Federica , Saumell Carlos , Ceballos Laura , Domínguez Paula , Guerrero Inés , Junco Milagros , Iglesias Lucía , Fernández Silvina
Published: January 2024

Nematophagous fungi are a biological control tool used against gastrointestinal nematodes in livestock. These fungi prey on free-living larvae in faeces and could be affected by active drugs excreted post-treatment. This study aimed to determine in vitro and under environmental conditions the effect of the following anthelmintics on the fungus Duddingtonia flagrans: ivermectin, levamisole, albendazole, fenbendazole and ricobendazole. The in vitro effect of anthelmintics on fungal growth and predatory capacity was assessed in corn meal agar and coprocultures, respectively. Ivermectin (1, 2 and 10 ppm), fenbendazole (0.027, 0.054 and 1 ppm) and albendazole (1 ppm) significantly affected fungal development. The fungal efficacy against L3 was high in the control and levamisole coprocultures but decreased significantly in the presence of albendazole, fenbendazole, ricobendazole and ivermectin. The impact of levamisole on D. flagrans was further assessed under environmental conditions in autumn and winter; the fungal efficacy measured in faecal pats and the surrounding herbage was not affected by levamisole at any time. This study shows that using albendazole, fenbendazole, ricobendazole or ivermectin may compromise fungal activity, as these drugs affect the free-living stages of nematodes in faeces, but levamisole can be safely considered in parasite control strategies involving D. flagrans and anthelmintic treatments.
The Development of a Measuring System for Intraoral SpO2
Authors: Kashima Yuki , Onimaru Minako , Isogai Ryosuke , Kawai Noboru , Yoshida Yoshifumi , Maki Koutaro
Published: January 2024

Blood oxygen saturation (SpO2) is an essential indicator of a patient’s general condition. However, conventional measurement methods have some issues such as time delay and interference by ambient light. Improved measurement methods must be developed, and there are no reports on intraoral measurements of SpO2 using wearable devices. Therefore, we aimed to establish an intraoral SpO2 measurement method for the first time. Twelve healthy adults participated in this study. The following steps were taken: (1) to identify the optimal measurement location, mid-perfusion index (PI) values were measured at six places on the mucosa of the maxilla, (2) to validate the optimal measurement pressure, PI values were obtained at different pressures, and (3) using the proposed mouthpiece device, SpO2 values in the oral cavity and on the finger were analyzed during breath-holding. The highest PI values were observed in the palatal gingiva of the maxillary canine teeth, with high PI values at pressures ranging from 0.3 to 0.8 N. In addition, changes in SpO2 were detected approximately 7 s faster in the oral cavity than those on the finger, which is attributed to their proximity to the heart. This study demonstrates the advantage of the oral cavity for acquiring biological information using a novel device.
Ultra-Short-Term Offshore Wind Power Prediction Based on PCA-SSA-VMD and BiLSTM
Authors: Wang Zhen , Ying Youwei , Kou Lei , Ke Wende , Wan Junhe , Yu Zhen , Liu Hailin , Zhang Fangfang
Published: January 2024

In order to realize the economic dispatch and safety stability of offshore wind farms, and to address the problems of strong randomness and strong time correlation in offshore wind power forecasting, this paper proposes a combined model of principal component analysis (PCA), sparrow algorithm (SSA), variational modal decomposition (VMD), and bidirectional long- and short-term memory neural network (BiLSTM). Firstly, the multivariate time series data were screened using the principal component analysis algorithm (PCA) to reduce the data dimensionality. Secondly, the variable modal decomposition (VMD) optimized by the SSA algorithm was applied to adaptively decompose the wind power time series data into a collection of different frequency components to eliminate the noise signals in the original data; on this basis, the hyperparameters of the BiLSTM model were optimized by integrating SSA algorithm, and the final power prediction value was obtained. Ultimately, the verification was conducted through simulation experiments; the results show that the model proposed in this paper effectively improves the prediction accuracy and verifies the effectiveness of the prediction model.
An IoT System for Air Pollution Monitoring with Safe Data Transmission
Authors: Bobulski Janusz , Szymoniak Sabina , Pasternak Kamila
Published: January 2024

Air pollution has become a global issue due to rapid urbanization and industrialization. Bad air quality is Europe’s most significant environmental health risk, causing serious health problems. External air pollution is not the only issue; internal air pollution is just as severe and can also lead to adverse health outcomes. IoT is a practical approach for monitoring and publishing real-time air quality information. Numerous IoT-based air quality monitoring systems have been proposed using micro-sensors for data collection. These systems are designed for outdoor air quality monitoring. They use sensors to measure air quality parameters such as CO2, CO, PM10, NO2, temperature, and humidity. The data are acquired with a set of sensors placed on an electric car. They are then sent to the server. Users can subscribe to the list and receive information about local pollution. This system allows real-time localized air quality monitoring and sending data to customers. The work additionally presents a secure data transmission protocol ensuring system security. This protocol provides system-wide attack resiliency and interception, which is what existing solutions do not offer.
Application and Development of Chemical Heat Washing Technology in Petroleum Oily Sludge Treatment: A Review
Authors: Liu Huapeng , Wang Xiyuan , Zhai Youqian , Xu Tingting
Published: January 2024

Because of the requirements for accelerating the construction of a green, low-carbon, and recycling economy development system, the petrochemical industry has been paying attention to green and low-carbon transformations and innovations. Oily sludge is a type of bulk hazardous solid waste from various sources that significantly harms the environment. Chemical heat washing of oily sludge represents a large proportion of the treatment technology for oily sludge, which can be used individually and has many applications in joint treatment with other processes. However, research on the formulation of cleaning agents for this process has mainly focused on the surfactant at this stage, and many studies have demonstrated the secondary pollution of surfactant; thus, research on reducing or replacing surfactants as cleaning agents has been conducted. This review highlights the research progress of chemical heat washing of oily sludge according to the classification of cleaning agents and describes the reasons for the stabilization of currently recognized oily sludge, the chemical heat washing process, and the mechanism of oil–soil separation. Finally, the research direction for new emulsions as new cleaning agents to replace surfactants is set out.
Peer Support Provided by People with Intellectual and Developmental Disabilities: A Rapid Scoping Review to Develop a Toolkit for Inclusive Research
Authors: Pfeiffer Beth , Hallock Taye , Tomczuk Luke , Kramer Jessica
Published: January 2024

Inclusive research teams actively engage people with intellectual and developmental disabilities at all stages of research development, implementation, and dissemination. There is a dearth of research that specifically addresses the use of peer support in research engagement, yet research using peer support may provide a useful framework for engagement in inclusive research teams. A rapid scoping review was completed following the reporting guidelines for PRISMA-SCR. The scoping review identified five peer support roles (communication, sharing experiences, helping peers to learn, peer development, and creating a welcoming environment) and two types of support and accommodation for peer supporters (individual and environmental). The findings of the rapid scoping review aided in developing key sections of a Peer Support Toolkit to help people with intellectual and developmental disabilities engage in research to create more inclusive research teams and research that is informed directly by the needs of people with lived experience. The scoping review and toolkit were completed by an inclusive team.
Valorisation of Forest and Agriculture Residual Biomass—The Application of Life Cycle Assessment to Analyse Composting, Mulching, and Energetic Valorisation Strategies
Authors: Brás Isabel , Silva Elisabete , Raimondo Roberta , Saetta Raffaella , Mignano Vincenzo , Fabbricino Massimiliano , Ferreira José
Published: January 2024

The treatment of agricultural and forest residues (AFRs) has become an important issue nowadays, both to avoid improper management and for their enhancement. In the study area of Viseu (Portugal), the AFRs are taken to a Residual Biomass Collection Centre. These are valorised in a Biomass Power Plant to produce electricity. Two further processes could be implemented to valorise this biomass: mulching and composting. This study aims to understand the best strategy to enhance this type of biomass residual considering their environmental performance. The Life Cycle Assessment (LCA) was applied considering a cradle-to-grave approach. Different processes of all the technologies were analysed, and the data collected enabled a comparison of 11 environmental impact categories. The results show that composting is the best alternative, except for global warming and ozone layer depletion, and energy valorisation has the greatest impact on five of the considered categories. In the three processes, impacts are mainly associated with the production and shredding phases of the residual biomass, rather than the transportation stages, due to the short distances covered. In all cases, the value of the final product generates market consequences in terms of electricity and fertiliser production. In line with the aim of the study, its outcomes may provide scientific support to local decision makers in defining best practices in the management of the AFRs.
Impact of Autonomous Vehicles on Traffic Flow in Rural and Urban Areas Using a Traffic Flow Simulator
Authors: Fujiu Makoto , Morisaki Yuma , Takayama Jyunich
Published: January 2024

Autonomous vehicles have the potential to significantly improve modes of transportation, and many businesses and research facilities are developing such systems. Although there are studies on the social implementation of autonomous vehicles, these studies are based on limited conditions such as predetermined driving environments. Therefore, in this study, we target urban areas and rural areas, and we simulate a behavioral algorithm for autonomous vehicles being developed and owned by Kanazawa University. In this study, a traffic flow simulation system (Aimsun) was constructed to reproduce the current situation of traffic flow in the city during normal times, using data from a person-trip survey conducted by the local government. In addition, we varied the mixing rate of automated vehicles and evaluated its effect on the delay time between ODs. We assume the gradual replacement of existing vehicles by autonomous vehicles on actual road networks and for realistic traffic volumes, and we investigate their impact on traffic flow. We vary the mixing rate of autonomous vehicles into actual traffic environments, and we measure the delay in the origin-destination (OD) interval to evaluate the impact of autonomous vehicles on traffic flow. The results obtained show that as the mixing rate of autonomous vehicles increases, the delay between OD intervals increases. Then, once the mixing rate exceeds a certain value, the delay between OD intervals gradually decreased. The delay time for all vehicles slightly increases as the mixing rate of autonomous vehicles increased from 10 to 45%. When the mixing rate increased from 45 to 50%, the delay time for all vehicles decreased notably, and when the mixing rate was 50 to 100%, it remained constant. Analytical results showed that when socially implementing autonomous vehicles, their mixing rate impacts the traffic flow; thus, there is a need to determine appropriate distribution scenarios and areas for implementation.