bluuebunny/crossref_metadata_2025 huggingface dataset:
Use of Decellularized Bio-Scaffolds for the Generation of a Porcine Artificial Intestine
Authors: Arcuri Sharon , Pennarossa Georgia , Prasadani Madhusha , Gandolfi Fulvio , Brevini Tiziana A. L.
Published: September 2024

In recent years, great interest has been focused on the development of highly reproducible 3D in vitro models that are able to mimic the physiological architecture and functionality of native tissues. To date, a wide range of techniques have been proposed to recreate an intestinal barrier in vitro, including synthetic scaffolds and hydrogels, as well as complex on-a-chip systems and organoids. Here, we describe a novel protocol for the generation of an artificial intestine based on the creation of decellularized bio-scaffolds and their repopulation with intestinal stromal and epithelial cells. Organs collected at the local slaughterhouse are subjected to a decellularization protocol that includes a freezing/thawing step, followed by sequential incubation in 1% SDS for 12 h, 1% Triton X-100 for 12 h, and 2% deoxycholate for 12 h. At the end of the procedure, the generated bio-scaffolds are repopulated with intestinal fibroblasts and then with epithelial cells. The protocol described here represents a promising and novel strategy to generate an in vitro bioengineered intestine platform able to mimic some of the complex functions of the intestinal barrier, thus constituting a promising 3D strategy for nutritional, pharmaceutical, and toxicological studies.
Fluoride-Treated Nano-HZSM-5 Zeolite as a Highly Stable Catalyst for the Conversion of Bioethanol to Propylene
Authors: Zhou Jian , Zhang Ni , Meng Tao , Guo Qiangsheng , Xue Zhaoteng , Mao Dongsen
Published: September 2024

Fluoride treatment of ZSM-5 zeolite can effectively adjust surface acidity and generate a secondary pore structure. In this study, a series of modified nano-HZSM-5 zeolites were prepared by NH4F-HF mixed solution treatment and applied to the selective conversion of bioethanol to propylene at 500 °C, atmospheric pressure, and a WHSV of 10 h−1. The results showed that NH4F-HF modification weakened the surface acidity of nano-HZSM-5 zeolites, thus inhibiting coke formation. Additionally, the mesopores in the nano-HZSM-5 zeolites increased after NH4F-HF treatment, thereby enhancing the mass transfer rate and improving the coke-resistance ability. The NH4F-HF mixed solution modification significantly improved the stability of nano-HZSM-5 zeolites in catalyzing bioethanol to propylene and greatly extended the working life of nano-HZSM-5 zeolites. It can be seen from the characterization of the deactivated catalysts that coke deposition and weakening of acidity may be the key factors for catalyst deactivation.
Evaluation of Accidental and Intentional Pediatric Poisonings: Retrospective Analysis of Emergency Medical Service Interventions in Wroclaw, Poland
Authors: Wojciechowki Jakub , Czapla Michał , Konop Marek , Juárez-Vela Raúl , Rosińczuk Joanna
Published: September 2024

Background/Objectives: Poisonings among children are a major cause of morbidity and mortality worldwide and present a significant challenge for emergency medical services (EMS). The aim of this study was to analyze the types of substances causing poisonings and the intention of poisonings in children, providing detailed information on the most common causes of poisonings in different age groups. Methods: A retrospective study was conducted analyzing medical records of pediatric patients under the care of emergency medical services (EMS) in Wroclaw, Poland, between 2015 and 2017. The study included all patients under 18 years old diagnosed with poisoning. Data collected included age, sex, cause of poisoning, location of the incident, neurological status (GCS), and the type of healthcare facility. Results: The study included 484 patients, with a mean age of 13 years. The largest age group was 16–18 years (44%), and the majority were female (58%). The most common causes of poisonings were alcohol (29.3%), medications (26%), and intoxicants (24.8%). Over half of the incidents occurred at home (52.5%). Intentional poisonings constituted 75% of cases, particularly involving alcohol (38.6%), intoxicants (32%), and medications (26.7%). Accidental poisonings were mainly due to household chemicals (28.7%) and carbon monoxide (27%). The differences in causes and locations of poisonings were statistically significant (p < 0.001). Conclusions: Our study showed that the most common causes of poisonings among children were alcohol, medications, and intoxicants. Poisonings most frequently occurred at home, especially with medications, while outside the home, alcohol and intoxicants were predominant. This study was not registered.
Interventions to Relieve the Burden on Informal Caregivers of Older People with Dementia: A Scoping Review
Authors: Encinas-Monge Celia , Hidalgo-Fuentes Sergio , Cejalvo Elena , Martí-Vilar Manuel
Published: September 2024

Dementia increases dependence in older adults and decreases their quality of life and that of their family members. These family members often take on the responsibility of caregiving and suffer from burden and health deterioration due to facing various stressors. The aim is to verify the effectiveness of existing interventions aimed at relieving the burden and stress of informal caregivers of older people with dementia. A scoping review was conducted by consulting the Web of Science, Scopus, ProQuest, and PubMed databases, following the guidelines of the PRISMA 2020 Statement. The review protocol has been registered in PROSPERO under number CRD42024558609. Twenty-six articles met the inclusion criteria and were reviewed, studying the type of intervention design, the sample size of caregivers and their main characteristics, the duration and follow-up, and the variables investigated with their respective measurement instruments. The analysis of the different studies showed that the most developed types of interventions are psychoeducational and cognitive-behavioral therapies, both individual and group. These interventions were mainly effective in reducing the burden and depressive symptoms of caregivers. It is considered advisable to implement more randomized controlled trials for further research, because as the number of caregivers increases, so does the need for affordable and effective interventions.
Self-Perceived Competencies and Attitudes on Palliative Care in Undergraduate Nursing Students: A Multicenter Descriptive Study
Authors: Lo Iacono Cinzia , Amodio Emanuele , Vella Giuseppe , Caruso Maria , D’Anna Giuseppe , Gambera Angelo , Soresi Maurizio , Intravaia Giuseppe , Latina Roberto
Published: September 2024

Introduction: Caring for the dying can generate anxiety and emotional distress, particularly in nursing students, and perceived competence could play a crucial role in enabling nurses to perform their duties with greater confidence. Unfortunately, few studies describe the relationship between students’ nursing attitudes and perceived self-efficacy in palliative care (PC). To overcome this gap, this survey aimed to assess the attitudes towards dying patients and the perceived competence of nursing students in palliative care at different universities in the south of Italy. Methods: A cross-sectional study was conducted from September 2022 to March 2023 involving nursing students from the three major Sicilian universities (Italy). The study included a survey investigating socio-demographic characteristics, palliative care training, knowledge about pain management, and previous experience with dying. Moreover, the Professional Competence of the Core Curriculum in Palliative Care Nursing (CCPCN) questionnaire and the Frommelt Attitudes Toward Care of the Dying—B Italian version (FATCOD-B-I) assessed competencies and emotional attitudes. Results: A total of 1913 nursing students were recruited, of which 71.3% were females, and 53.9% were in the age range of 18 to 21 years. In the multivariable analysis, practical PC training was a substantial factor in enhancing competencies (Adj-OR 2.78 [95% CI = 2.12–3.65]). Male students had higher competence odds (Adj-OR 1.38 [95% CI = 1.14–1.66]), and perceived knowledge strongly correlated with self-assessed competence. Advancement in academic years also positively influenced competence self-assessment (Adj-OR 1.98 [95% CI = 1.75–2.24]). Regarding emotional attitudes, a per-quartile increase in competence score was found to improve the attitude score (Adj-OR 1.24 [95% CI = 1.13–1.35]). Conclusions: Nursing students gain valuable experience during clinical experience. PC training and perceived knowledge of PC significantly increase nurses’ competencies, and the latter seem to be strongly associated with attitudes. Thus, introducing palliative care education into nurses’ core curricula could be a way to reduce anxiety and emotional distress in young students.
Media Health Literacy in Spanish Nursing Students: A Multicenter Cross-Sectional Study
Authors: Navas-Echazarreta Noelia , Satústegui-Dordá Pedro José , Rodríguez-Velasco Francisco José , García-Perea María Eva , Martínez-Sabater Antonio , Chover-Sierra Elena , Ballestar-Tarín María Luisa , Del Pozo-Herce Pablo , González-Fernández Silvia , de Viñaspre-Hernández Regina Ruiz , Czapla Michal , Juárez-Vela Raúl
Published: September 2024

Background: Amidst current misinformation, media literacy is an essential competency for nursing professionals. This study aimed to analyze the level of media health literacy among Spanish undergraduate nursing students, stratifying the results by gender, region, and other associated factors. Methods: A cross-sectional study was conducted at five Spanish universities (N = 416) using the Spanish version of the Media Health Literacy (MeHLit-SV) Questionnaire. Results: Students presented an average media health literacy score of 48.73 points. The media health literacy level was significantly higher among students from universities in the central and northeastern regions of Spain (ANOVA Test, p = 0.0002), those who had previously studied in a city (ANOVA Test, p = 0.001), those who combined their studies with employment (ANOVA Test, p = 0.001), and those residing in communities with fewer than 500 inhabitants (ANOVA Test, p = 0.001). No differences were found based on gender. Conclusions: The media health literacy level of the students was deficient and varied according to socio-economic and socio-educational factors. Understanding the literacy level of future nurses and promoting the inclusion of this competency in their education will enable them to become leaders in improving the population’s health self-care.
Innovation Support Reduces Quiet Quitting and Improves Innovative Behavior and Innovation Outputs among Nurses in Greece
Authors: Moisoglou Ioannis , Katsiroumpa Aglaia , Prasini Ioanna , Gallos Parisis , Kalogeropoulou Maria , Galanis Petros
Published: September 2024

Background: Innovation is a crucial issue in healthcare services since it can affect job-related variables such as productivity, satisfaction, and burnout. The aim of our study was to examine the impact of innovation support on quiet quitting, innovative behavior, and innovation outputs among nurses. Methods: We conducted a cross-sectional study in Greece during April 2024. We employed a convenience sample of nurses. We followed the reporting of observational studies in epidemiology (STROBE). We used the following instruments: (a) the innovation support inventory (ISI) to measure innovation support; (b) the quiet quitting scale (QQS) to measure quiet quitting; (c) the innovative behavior inventory (IBI) to measure innovative behavior; and (d) the innovation outputs (IO) scale to measure innovation outputs. Our study questionnaire was anonymous, and nurses gave their informed consent to participate. The Ethics Committee of the Faculty of Nursing, National and Kapodistrian University of Athens, approved our study protocol, while we followed the guidelines of the Declaration of Helsinki. Results: Our study population included 328 nurses with a mean age of 42.3 years (standard deviation: 9.7). Among them, 89.9% were females. Our multivariable analysis identified a negative relationship between innovation support and quiet quitting. Moreover, we found that managerial support and cultural support improved several aspects of innovative behavior, such as idea generation, idea search, idea communication, implementation starting activities, involving others, and overcoming obstacles. Additionally, managerial support improved innovation outputs. Conclusions: Our findings suggested the positive impact of innovation support on quiet quitting, innovative behavior, and innovation outputs among nurses. Organizations and nurses’ managers should establish an innovative working environment to improve nurses’ passion, motives, and productivity.
The Impact of Pain, Threat Perception and Emotional Distress on Suicide Risk in Individuals with Colorectal Cancer
Authors: Espuig Alba , Pons-Vinent Maria , Carbajo Eva , Lacomba-Trejo Laura
Published: September 2024

Background: Colorectal cancer (CRC) can significantly impact mental health, increasing suicide risk. Variables such as pain and threat perception may be crucial. This study aims to identify predictors of suicide risk in individuals with CRC. Methods: A total of 71 participants (76.06% men) aged 27 to 88 years (M = 65.18, SD = 12.02) were assessed using the SF-36 for pain, the Brief Illness Perception Questionnaire (B-IPQ) for threat perception, the Hospital Anxiety and Depression Scale (HADS) for emotional distress, and the Plutchik suicide risk scale for suicide risk. Descriptive, comparative, and correlational analyses were performed, followed by a linear regression analysis. Results: Nearly 20% of participants exhibited high suicide risk. There was a moderate-to-high association between suicide risk and the perception of threat, pain, and emotional distress. The linear regression model explained 39% of the variance in suicide risk, with threat perception, pain, and emotional distress as significant predictors. Conclusions: These results indicate the need for multidisciplinary care for individuals with CRC, including emotional support from health psychologists, and holistic, human-centered care from nursing and medical professionals. Future research is necessary to further explore these relationships and improve patient care strategies.
Molecular Mechanisms of Drug Resistance in Leishmania spp.
Authors: Moncada-Diaz Maria Juliana , Rodríguez-Almonacid Cristian Camilo , Quiceno-Giraldo Eyson , Khuong Francis T. H. , Muskus Carlos , Karamysheva Zemfira N.
Published: September 2024

The protozoan parasite Leishmania causes leishmaniasis, a neglected tropical disease, that disproportionately affects underdeveloped countries. This disease has major health, economic, and social implications, particularly because of the limited treatment options, high cost, the severe side effects associated with available therapeutics, and the high rate of treatment failure caused by the parasites’ growing resistance to current medications. In this review, we describe first the common strategies used by pathogens to develop drug resistance and then focus on the arsenal of available drugs to treat leishmaniasis, their modes of action, and the molecular mechanisms contributing to drug resistance in Leishmania spp., including the role of genomic, transcriptional, and translational control. We focus more specifically on our recent discovery of translational reprogramming as a major driver of drug resistance leading to coordinated changes in the translation of transcripts and orchestrating changes in metabolome and lipidome to support drug resistance. A thorough understanding of these mechanisms is essential to identify the key elements needed to combat resistance and improve leishmaniasis treatment methods.
Off-Label Pediatric Medication Prescribing and Dispensing: Awareness and Attitudes among Community Pharmacists: A Questionnaire-Based Study
Authors: Jîtcă Carmen-Maria , Jîtcă George , Silvia Imre
Published: September 2024

Off-label practice in pediatrics requires relentless engagement from all the health professionals involved. Community pharmacists are the last ones in the prescribing–dispensing chain; therefore, they have the key responsibility of verifying the correctness of a treatment. A cross-sectional study was conducted for assessing the awareness and views of Romanian community pharmacists, regarding off-label drugs in the pediatric population, through a 28-item questionnaire comprising five sections of different topics (general knowledge, frequency of prescribing and dispensing off-label medication, views, and attitudes). The sample size was 236 questionnaires with a response rate of 41.11%. A statistical analysis of the obtained data was performed with GraphPad Prism v.9. The results indicate that 55.1% of the community pharmacists have a good general knowledge and awareness regarding the off-label practice, although the legal frame is unclear. The responses highlight a high frequency of prescribing and request of medication for respiratory conditions (45.3%) and antibiotics (23.5%), with a concerning gap regarding the adverse events related to the off-label treatments (56.7%). A very small percentage of pharmacists (7.1%) contact a fellow healthcare professional when encountering an off-label prescription. In conclusion, in addition to the pharmacist’s conduct towards the best interest of the patient, there is a clear need to improve the doctor–pharmacist collaboration in order to make an off-label treatment successful.
Enhanced Thermoacoustic Imaging System with Parallel Ultrasonic Velocity Measurement for Distinguishing Types of Microwave-Absorbing Anomalies
Authors: Ding Wenzheng , Zhang Yao
Published: September 2024

Microwave-absorbing suspicious objects (MASOs) found using microwave-induced thermoacoustic imaging (MTI) can be divided into two types—endogenous (such as tumors or hematoceles) and exogenous (such as calculi or foreign bodies). These have different microwave absorption or ultrasonic velocity than normal human tissue, so MTI is efficient in detecting these anomalies. However, the existing MTI techniques can only reflect morphological information, making it difficult to distinguish the type of each anomaly. In this paper, a newly enhanced MTI system composed of a multiple-element ring transducer and a parallel data acquisition system (DAS) is presented. By using ultrasonic velocity and microwave absorption measurements, where the ultrasonic velocity is mainly used as an additional parameter to reflect mechanical characteristics, the type of the detected anomaly can be identified. In our experiments, the MASO can be located through the absorption difference detected by MTI. Due to the use of multiple-element transducers and a parallel DAS, the raw data can be acquired within about 20 ms for a two-dimensional image. Additionally, the ultrasonic velocity of the MASO can be calculated from the time sequence diagram of ultrasound propagation with a maximum time error of 0.084 μs. Apart from distinguishing the type of the anomaly, the proposed ultrasonic velocity-assisted microwave-induced thermoacoustic imaging (US-MTI) system has other advantages, such as being noninvasive, and allowing rapid imaging and a large field of view, which make US-MTI a suitable modality for regular screening.
General Inverse Problem Solution for Two-Level Systems and Its Application to Charge Transfer
Authors: Migliore Agostino , Nakazato Hiromichi , Sergi Alessandro , Messina Antonino
Published: September 2024

Two-level quantum systems are building blocks of quantum technologies, where the qubit is the basic unit of quantum information. The ability to design driving fields that produce prespecified evolutions of relevant physical observables is crucial to the development of such technologies. Using vector algebra and recently developed strategies for generating solvable two-level Hamiltonians, we construct the general solution to the inverse problem for a spin in a time-dependent magnetic field and its extension to any two-level system associated with fictitious spin and field. We provide a general expression for the field that drives the dynamics of the system so as to realize prescribed time evolutions of the expectation values of the Pauli operators and the autocorrelation of the Pauli vector. The analysis is applied to two-state charge transfer systems, showing that the charge transfer process can be seen as a motion of the state of the associated fictitious qubit on the Bloch sphere, and that the expectation values of the related Pauli operators describe the interference between the two differently localized electronic states and their population difference. Our formulation is proposed as a basic step towards potential uses of charge transfer in quantum computing and quantum information transfer.
Biochemical and Proteomic Analyses in Drought-Tolerant Wheat Mutants Obtained by Gamma Irradiation
Authors: Şen Ayşe , Gümüş Tamer , Temel Aslıhan , Öztürk İrfan , Çelik Özge
Published: September 2024

The bread wheat cultivar (Triticum aestivum L. cv. Sagittario) as a parental line and its mutant, drought-tolerant lines (Mutant lines 4 and 5) were subjected to polyethylene glycol (PEG)-induced drought. Drought stress resulted in decreased chlorophyll levels and the accumulation of proline and TBARS, despite increases in activities of catalase, peroxidase, and superoxide dismutase enzymes. Transcription of the genes encoding these enzymes and delta-1-pyrroline 5-carboxylase synthetase was induced by drought. 2-DE gel electrophoresis analysis identified differentially expressed proteins (DEPs) in the mutant lines, which are distinguished by “chloroplast”, “mitochondrion”, “pyruvate dehydrogenase complex”, and “homeostatic process” terms. The drought tolerance of the mutant lines might be attributed to improved photosynthesis, efficient ATP synthesis, and modified antioxidant capacity. In addition to proteomics data, the drought tolerance of wheat genotypes might also be assessed by chlorophyll content and TaPOX gene expression. To our knowledge, this is the first proteomic analysis of gamma-induced mutants of bread wheat. These findings are expected to be utilized in plant breeding studies.
Isolation and Plant Growth Promotion Effect of Endophytic Siderophore-Producing Bacteria: A Study on Halophyte Sesuvium portulacastrum
Authors: Cen Xinyi , Li Hua , Zhang Yanhua , Huang Lingfeng , Luo Yuanrong
Published: September 2024

The objective of the present study was to isolate endophytes from the roots of the halophyte Sesuvium portulacastrum, which is applied for aquatic phytoremediation. From these endophytes, siderophore-producing bacteria were specifically isolated for their potential capacity to promote plant growth. The siderophore production capacity of the isolated bacteria was quantified, and a high-yield siderophore-producing strain was selected for further investigation. A total of 33 endophytic bacteria were successfully isolated and identified using a culturable approach. Of these, 10 siderophore-producing bacteria were identified using the selective agar assay, displaying siderophore unit (SU) values ranging from 11.90% to 80.39%. It is noteworthy that Erwinia sp. QZ-E9 exhibited the highest siderophore production capacity, achieving an SU of 80.39%. A microcosm co-cultivation experiment was conducted with the strain QZ-E9 in iron-deficient conditions (2 μmol/L Fe3⁺). The results demonstrated that strain QZ-E9 significantly enhanced the growth of S. portulacastrum, by increases in both fresh weight (1.41 g) and root length (18.7 cm). Furthermore, fluorescence in situ hybridization (FISH) was utilized to ascertain the colonization pattern of strain QZ-E9 within the plant roots. The analysis demonstrated that strain QZ-E9 exhibited extensive colonization of the epidermal and outer cortical cells of S. portulacastrum roots, as well as the intercellular spaces and vascular tissues. This colonization indicated that Erwinia sp. QZ-E9 plays a crucial role in promoting the growth of S. portulacastrum, presumably through its siderophore-mediated iron acquisition mechanism.
FtMYB163 Gene Encodes SG7 R2R3-MYB Transcription Factor from Tartary Buckwheat (Fagopyrum tataricum Gaertn.) to Promote Flavonol Accumulation in Transgenic Arabidopsis thaliana
Authors: Du Hanmei , Ke Jin , Sun Xiaoqian , Tan Lu , Yu Qiuzhu , Wei Changhe , Ryan Peter R. , Wang An’hu , Li Hongyou
Published: September 2024

Tartary buckwheat (Fagopyrum tataricum Gaertn.) is a coarse grain crop rich in flavonoids that are beneficial to human health because they function as anti-inflammatories and provide protection against cardiovascular disease and diabetes. Flavonoid biosynthesis is a complex process, and relatively little is known about the regulatory pathways involved in Tartary buckwheat. Here, we cloned and characterized the FtMYB163 gene from Tartary buckwheat, which encodes a member of the R2R3-MYB transcription factor family. Amino acid sequence and phylogenetic analysis indicate that FtMYB163 is a member of subgroup 7 (SG7) and closely related to FeMYBF1, which regulates flavonol synthesis in common buckwheat (F. esculentum). We demonstrated that FtMYB163 localizes to the nucleus and has transcriptional activity. Expression levels of FtMYB163 in the roots, stems, leaves, flowers, and seeds of F. tataricum were positively correlated with the total flavonoid contents of these tissues. Overexpression of FtMYB163 in transgenic Arabidopsis enhanced the expression of several genes involved in early flavonoid biosynthesis (AtCHS, AtCHI, AtF3H, and AtFLS) and significantly increased the accumulation of several flavonoids, including naringenin chalcone, naringenin-7-O-glucoside, eriodictyol, and eight flavonol compounds. Our findings demonstrate that FtMYB163 positively regulates flavonol biosynthesis by changing the expression of several key genes in flavonoid biosynthetic pathways.
Photosynthetic Performance of Oil Palm Genotypes under Drought Stress
Authors: Montoya Carmenza , Daza Edison , Mejía-Alvarado Fernan Santiago , Caicedo-Zambrano Arley Fernando , Ayala-Díaz Iván , Ruiz-Romero Rodrigo , Romero Hernán Mauricio
Published: September 2024

Water deficiency and potential drought periods could be important ecological factors influencing cultivation areas and productivity once different crops are established. The principal supply of vegetable oil for oil crops is oil palm, and new challenges are emerging in the face of climatic changes. This study investigated the photosynthetic performance of 12 genotypes of Elaeis exposed to drought stress under controlled conditions. The assay included genotypes of Elaeis guineensis, Elaeis oleifera, and the interspecific O×G hybrid (E. oleifera × E. guineensis). The principal results showed that the E. guineensis genotype was the most efficient at achieving photosynthesis under drought stress conditions, followed by the hybrid and E. oleifera genotypes. The physiological parameters showed good prospects for vegetal breeding with different O×G hybrids, mainly because of their ability to maintain the equilibrium between CO2 assimilation and stomatal aperture. We validated 11 genes associated with drought tolerance, but no differences were detected. These results indicate that no allelic variants were represented in the RNA during sampling for the validated genotypes. In conclusion, this study helps to define genotypes that can be used as parental lines for oil palm improvement. The gas exchange data showed that drought stress tolerance could define guidelines to incorporate the available genetic resources in breeding programs across the early selection in nursery stages.
Quantifying Genetic Parameters for Blackleg Resistance in Rapeseed: A Comparative Study
Authors: Bocianowski Jan , Starosta Ewa , Jamruszka Tomasz , Szwarc Justyna , Jędryczka Małgorzata , Grynia Magdalena , Niemann Janetta
Published: September 2024

Selection is a fundamental part of the plant breeding process, enabling the identification and development of varieties with desirable traits. Thanks to advances in genetics and biotechnology, the selection process has become more precise and efficient, resulting in faster breeding progress and better adaptation of crops to environmental challenges. Genetic parameters related to gene additivity and epistasis play a key role and can influence decisions on the suitability of breeding material. In this study, 188 rapeseed doubled haploid lines were assessed in field conditions for resistance to Leptosphaeria spp. Through next-generation sequencing, a total of 133,764 molecular markers (96,121 SilicoDArT and 37,643 SNP) were obtained. The similarity of the DH lines at the phenotypic and genetic levels was calculated. The results indicate that the similarity at the phenotypic level was markedly different from the similarity at the genetic level. Genetic parameters related to additive gene action effects and epistasis (double and triple) were calculated using two methods: based on phenotypic observations only and using molecular marker observations. All evaluated genetic parameters (additive, additive-additive and additive-additive-additive) were statistically significant for both estimation methods. The parameters associated with the interaction (double and triple) had opposite signs depending on the estimation method.
Challenges Associated with the Production of Nanofibers
Authors: Maduna Lebo , Patnaik Asis
Published: September 2024

Nanofibers, with their high surface area-to-volume ratio and unique physical properties, hold significant promise for a wide range of applications, including medical devices, filtration systems, packaging, electronics, and advanced textiles. However, their development and commercialization are hindered by several key challenges and hazards. The main issues are production cost and yield, high voltage, clogging, and toxic materials driven by complex production techniques, which limit their adoption. Additionally, there are environmental and health concerns associated with nanofiber production and disposal, necessitating the development of safer and more sustainable processes and materials. Addressing these challenges requires continued innovation in materials science and industrial practices, as well as a concerted effort to balance production, material, and surrounding condition parameters. This study emphasizes the challenges and hazards associated with nanofiber materials and their production techniques, including electrospinning, centrifugal spinning, solution blow spinning, electro-blown spinning, wet spinning, and melt spinning. It also emphasizes biopolymers and recycling as sustainable and eco-friendly practices to avoid harming the environment and human beings.
Harnessing Chitosan Beads as an Immobilization Matrix for Zero-Valent Iron Nanoparticles for the Treatment of Cr(VI)-Contaminated Laboratory Residue
Authors: Rychluk Ignacio Daniel , Casado Ulises , Montesinos Víctor Nahuel , Quici Natalia
Published: September 2024

Nanocomposites (NCs) consisting of zero-valent iron nanoparticles (nZVI) immobilized in chitosan (CS) were prepared and employed for the removal of hexavalent chromium (Cr(VI)) from both synthetic and real wastewater. Medium (MCS)- and high (HCS)-molecular-weight chitosan and stabilization with carboxymethylcellulose (CMC) and different nZVI loads were explored. Characterization through scanning electron microscopy with energy dispersive X-ray analysis (SEM-EDS) and X-ray diffraction (XRD) revealed millimeter-sized spheres with micrometer-sized nZVI clusters randomly distributed. Better nanoparticle dispersion was observed in NCs from the CMC-MCS and HCS combinations. Fourier-transform infrared spectroscopy (FTIR) analysis indicated that CS binds to Fe(II) or Fe(III) on the surface of nZVI through its functional groups -CONH-, -N-H, and -C-OH and through the -COO− functional group of CMC, forming a bidentate bridge complex. Through experiments with synthetic waters, it was found that the elimination of Cr(VI) was favored by lowering the pH, obtaining the maximum percentage of Cr(VI) removal at pH 5.5. With real waters, it was shown that increasing the mass of NCs also improved the removal of Cr(VI), following a pseudo-second-order adsorption kinetics. The synthesized materials show great potential for applications in environmental remediation, showing good efficiency in the removal of Cr(VI) in wastewater.
Stage Definition and Recovery Calibration of Complex High-Water Cut Gas Wells in S Gas Field
Authors: Ning Pengzhan , Nie Xiangrong , Deng Dingtian , Xiong Jiao , Wei Benchi
Published: September 2024

The exploration and development of tight gas is a significant area of interest, yet the efficient development of this resource still faces considerable challenges. Statistical data indicate that China possesses approximately 1.5 × 1012 m3 of proven dense gas reserves, which are concentrated in areas with high water content and a low abundance of pores. This makes it challenging to utilise these reserves effectively and to extract them in a cost-effective manner. As exploration and development continue, numerous challenges emerge, including low production capacity, significant discrepancies between single gas wells, uncertain control reserves of horizontal gas wells, and the unclear distribution law of the remaining gas. In light of the aforementioned issues, this study examined a representative dense and high-water-bearing gas reservoir in the D gas wells region of the S gas field. The gas wells within the target gas block were classified and analysed. By elucidating the seepage mechanism of the dense and high-water-bearing gas reservoir and integrating it with the material balance equation, a recovery prediction method for the dense and high-water-bearing gas reservoir was devised.
Fucosylated Chondroitin Sulfate from Bohadschia ocellata: Structure Analysis and Bioactivities
Authors: Thinh Pham Duc , Cao Hang Thi Thuy , Trung Dinh Thanh , Minh Duong Khanh , Cao Thao Quyen , Van Tran Thi Thanh , Zueva Anastasia O. , Ermakova Svetlana P. , Nguyen Thanh-Danh
Published: September 2024

Fucosylated chondroitin sulfate (FCS) was prepared from Bohadschia ocellata using protease hydrolysis. The structural characteristics of FCS were confirmed through chemical composition analysis using FTIR spectroscopy, 1H NMR, and 13C NMR. FCS from B. ocellata (FCS-Bo) exhibited an average molecular weight of approximately 122 kDa. The biological activities of FCS-Bo, including anticoagulant, anti-cancer, and Protein Tyrosine Phosphatase 1B (PTP1B) inhibition, were evaluated. FCS-Bo displayed potent anticoagulant properties, markedly extending activated partial thromboplastin time, prothrombin time, and thrombin time when compared to the heparin control. In anti-cancer bioactivity research, FCS-Bo efficiently inhibited colony formation in the colon cancer cell lines HCT-116, HT-29, and DLD-1, achieving inhibition rates of up to 65%. Additionally, FCS-Bo exhibited significant inhibition of PTP1B, with an IC50 as low as 0.0326 µg/mL, suggesting its potential for improving insulin sensitivity and managing conditions such as type 2 diabetes and obesity.
The Antimicrobial Effect of the Incorporation of Inorganic Substances into Heat-Cured Denture Base Resins—A Systematic Review
Authors: Lima Mariana , Salgado Helena , Correia André , Fonseca Patrícia
Published: September 2024

Introduction: Polymethylmethacrylate (PMMA) is the most widely used denture base material due to its favourable properties. Several studies have tested the incorporation of anti-infective agents into PMMA as a strategy to prevent biofilm growth on the denture surface. This systematic review aims to evaluate the efficacy of incorporating inorganic antimicrobial particles into denture base resins in preventing antimicrobial growth, thereby identifying the most effective agents for enhancing PMMA’s antimicrobial properties. Materials and methods: This systematic review followed the PRISMA guidelines, and the research protocol was registered in PROSPERO. The search was performed by using Medical Subject Headings and free text combined with Boolean operators in PubMed/Medline® and in Cochrane® and a free text combination in Web of Science® Core Collection. Data regarding the inorganic particles studied, their antimicrobial effect, and the type of samples produced were collected and analysed. Results: After screening, a total of fifteen studies were included in this review. Most samples were disk-shaped and of varying sizes, and the most tested microbial strain was Candida albicans. Silver was the most used antimicrobial particle, followed by gold, titanium, and copper. Conclusions: Overall, incorporating inorganic particles into PMMA has produced promising antimicrobial results, depending on the concentration. Due to the high heterogeneity observed in the samples, more studies are recommended, particularly clinical trials.
Craniosacral Breathing in a Removable Partial Denture: A Novel Approach to Building a Denture
Authors: Ceraulo Saverio , Cremonini Francesca , Barbarisi Antonio , Lauritano Dorina , Caccianiga Gianluigi , Biagi Roberto
Published: September 2024

Craniosacral rhythm is a manual therapy technique that focuses on the subtle, rhythmic movement of cerebrospinal fluid as it flows through the central nervous system and musculoskeletal system. Through light and delicate manipulation of the cranial bones, membranes and soft tissues of the head and neck, it promotes rebalancing and release from tension in the body, improving the circulation of the cerebrospinal fluid and the individual’s health. In the field of prosthetic dentistry, in rare cases, such as a full arch or removable partial denture, the patient perceives a sensation of constriction even though all procedures have been used correctly. In this work, a new design fora removable partial denture is proposed that does not interfere with the primary respiratory mechanism, reducing the sensation of constriction in the patient. The materials used are the same as those used for a normal removable partial denture, and the technique used is the “lost wax” one: the novelty is that the prosthesis is made in two separate parts that are joined by a central connection; the result is a removable partial denture with a small central thickening in the main joint represented by the connection of the two pieces. Craniosacral breathing movements are favored with this prosthesis as the metal structure is hyperstatic towards intrusion movements, more or less virtual in the opposite direction, i.e., extrusion towards the vestibule.
Epigraphy and New Testament Exegesis
Authors: Hill Deborah , Oehler Markus
Published: September 2024

Within the diverse paths of New Testament exegesis, a new approach is presented here, namely, interpretation against the background of epigraphic sources. Although this approach has a prehistory in the 19th and 20th centuries, it is only now being taken up again with the project of an Epigraphical Commentary on the New Testament (ECNT). The article briefly describes the more precise procedure for compiling such a commentary and presents three examples from different areas of the New Testament to illustrate the types of insights that can be gained from inscriptions: on κατάκριμα (Rom 5:15, 18; 8:1); on the statement that someone is bound or in bonds (Phlm); and on the meaning of δικαιοσύνη as a virtuous quality in inscriptions, which influences interpretation of 1Tim, Mt, and Luke-Acts. The authors argue for recognizing the critically important role inscriptions in particular can play in illuminating the language and culture of the Mediterranean in the first century, and thus also of early Christian texts.
Assessing the Impact of Aridity on Argan Trees in Morocco: Implications for Conservation in a Changing Climate
Authors: Afi Chaima , Telmoudi Maryem , Labbassi Said , Chabbi Naima , Hallam Jamal , Msanda Fouad , Ait Aabd Naima
Published: September 2024

Climate change, through increased aridity, threatens ecosystems, including Morocco’s endemic Argania Spinosa L. Skeels. This study assesses the impact of aridity on argan trees by analyzing morphological, ecophysiological, and biochemical parameters across various regions and comparing them with historical data. Significant variations were observed in leaf area, leaf length, chlorophyll content, relative water content, polyphenols, flavonoids, soluble sugars, and antioxidant activity, while leaf width ratio and chlorophyll ratio remained stable. Tioughza exhibited the largest leaf area (136.07 mm2), the highest chlorophyll content (436.76 mg/m2), and superior water retention (52.27%). Conversely, Ezzaouite showed the smallest leaf area (85.76 mm2) and lowest water content (37.68%). Increased aridity has intensified these differences, revealing the argan tree’s vulnerability to climate change. The findings underscore the need for targeted conservation efforts, including reforestation, strengthened legislation, and enhanced genetic research, to sustain this vital species.
RoboMan: An Adult-Sized Humanoid Robot with Enhanced Performance, Inherent Stability, and Two-Stage Balance Control to Facilitate Research on Humanoids
Authors: Mohammadi Vahid , Hosseini Mojtaba , Jafari Farhad , Behboodi Ahad
Published: September 2024

Creating an adult-sized humanoid robot with stable walking capabilities is a major challenge in robotics. While many renowned research groups focus on robots for perilous work environments and precision tasks, our approach simplifies balance control, making it accessible to robotics research groups and educational institutes. This facilitates the development of complex functionalities such as vision and object manipulation for adult-sized humanoids. This research article introduces RoboMan II, an advanced version of RoboMan I, which won the most prestigious award in all humanoid robot leagues at RoboCup 2016 due to its exceptional performance in walking and playing soccer. RoboMan II features significant improvements in performance, inherent stability, recovery after falls, and balance control. To facilitate its development, RoboMan II is lighter and incorporates a modified foot and parallel structure for its leg to boost its inherent stability, along with a two-stage balance control system for Immediate Response and Gradual Adaptation, enhancing its adaptability in various environments. Our simulation results demonstrate that RoboMan II’s walking stability on flat surfaces improved significantly in the face of minor perturbations, with the number of steps within the stable region increasing from 24%, with only the immediate controller to 58% when both controllers were used. Similar improvements were observed on inclined surfaces. Additionally, the 3D CAD files for all of the robot parts are released as open source in conjunction with this paper to facilitate reproduction and further innovation. The forthcoming RoboMan III will incorporate custom servo motors for increased speed, torque, and enhanced fall recovery, preventing disengagement of the gear box after a fall. It promises to be an invaluable asset for research and practical applications in humanoid robotics.
Dynamic Analysis and Risk Assessment of Vegetation Net Primary Productivity in Xinjiang, China
Authors: Zhang Wenjie , Zhao Xiang , Li Hao , Fang Yutong , Shi Wenxi , Zhao Siqing , Guo Yinkun
Published: September 2024

Vegetation net primary productivity (NPP) is a key indicator for assessing vegetation dynamics and carbon cycle balance. Xinjiang is located in an arid and ecologically fragile region in northwest China, but the current understanding of vegetation dynamics in the region is still limited. This study aims to analyze Xinjiang’s NPP spatial and temporal trends, using random forest regression to quantify the extent to which climate change and human activities affect vegetation productivity. CMIP6 (Coupled Model Intercomparison Project Phase 6) climate scenario data help assess vegetation restoration potential and future risks. Our findings indicate that (1) Xinjiang’s NPP exhibits a significant increasing trend from 2001 to 2020, with three-quarters of the region experiencing an increase, 2.64% of the area showing significant decrease (p < 0.05), and the Ili River Basin showing a nonsignificant decreasing trend; (2) precipitation and radiation are major drivers of NPP variations, with contribution ratios of 35.13% and 30.17%, respectively; (3) noteworthy restoration potential exists on the Tian Shan northern slope and the Irtysh River Basin, where average restoration potentials surpass 80% relative to 2020, while the Ili River Basin has the highest future risk. This study explores the factors influencing the current vegetation dynamics in Xinjiang, aiming to provide references for vegetation restoration and future risk mitigation, thereby promoting sustainable ecological development in Xinjiang.
Quality Evaluation of Multi-Source Cropland Data in Alpine Agricultural Areas of the Qinghai-Tibet Plateau
Authors: Lv Shenghui , Xia Xingsheng , Chen Qiong , Pan Yaozhong
Published: September 2024

Accurate cropland distribution data are essential for efficiently planning production layouts, optimizing farmland use, and improving crop planting efficiency and yield. Although reliable cropland data are crucial for supporting modern regional agricultural monitoring and management, cropland data extracted directly from existing global land use/cover products present uncertainties in local regions. This study evaluated the area consistency, spatial pattern overlap, and positional accuracy of cropland distribution data from six high-resolution land use/cover products from approximately 2020 in the alpine agricultural regions of the Hehuang Valley and middle basin of the Yarlung Zangbo River (YZR) and its tributaries (Lhasa and Nianchu Rivers) area on the Qinghai-Tibet Plateau. The results indicated that (1) in terms of area consistency analysis, European Space Agency (ESA) WorldCover cropland distribution data exhibited the best performance among the 10 m resolution products, while GlobeLand30 cropland distribution data performed the best among the 30 m resolution products, despite a significant overestimation of the cropland area. (2) In terms of spatial pattern overlap analysis, AI Earth 10-Meter Land Cover Classification Dataset (AIEC) cropland distribution data performed the best among the 10 m resolution products, followed closely by ESA WorldCover, while the China Land Cover Dataset (CLCD) performed the best for the Hehuang Valley and GlobeLand30 performed the best for the YZR area among the 30 m resolution products. (3) In terms of positional accuracy analysis, the ESA WorldCover cropland distribution data performed the best among the 10 m resolution products, while GlobeLand30 data performed the best among the 30 m resolution products. Considering the area consistency, spatial pattern overlap, and positional accuracy, GlobeLand30 and ESA WorldCover cropland distribution data performed best at 30 m and 10 m resolutions, respectively. These findings provide a valuable reference for selecting cropland products and can promote refined cropland mapping of the Hehuang Valley and YZR area.
Study on the Driver Visual Workload in High-Density Interchange-Merging Areas Based on a Field Driving Test
Authors: Zhang Yue , Jiang Pei , Wang Siqi , Cheng Shuang , Xu Jin , Liu Yawen
Published: September 2024

A visual workload model was constructed to determine and evaluate drivers’ visual workload characteristics in high-density interchange-merging areas. Five interchanges were selected, and a real-vehicle driving test was conducted with 47 participants. To address the differences in drivers’ visual characteristics in the interchange cluster merging areas, the Criteria Importance Through Intercriteria Correlation (CRITIC) objective weighting method was employed. Six visual parameters were selected to establish a comprehensive evaluation model for the visual workload in high-density interchange-merging areas. The results show that the average scanning frequency and average pupil area change rate are most strongly correlated with the visual workload, whereas the average duration of a single gaze has the lowest weight in the visual workload assessment system. Different driver visual workloads were observed depending on the environment of the interchange-merging areas, and based on these, recommendations are proposed to decrease drivers’ workload, thereby increasing road safety.