bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Mn Content on the Toughness and Plasticity of Hot-Rolled High-Carbon Medium Manganese Steel
Authors: Wang Menghu , Liang Xiaokai , Ren Wubin , Tong Shuai , Sun Xinjun
Published: March 2023

The tensile and impact deformation behavior of three different Mn content test steels, xMn-1.0C-0.25V-1.5Cr-0.3Mo (5, 8 and 13 wt%), were investigated using mechanical properties testing, SEM-EBSD and TEM. The elongation and −20 °C impact energy of the three types of Mn content test steels increased as the Mn content increased. The room temperature tensile elongation was 9%, 23% and 81%, and the −20 °C impact energy was 9 J, 99 J and 241 J, respectively. The fracture morphologies of 5 Mn and 8 Mn were found to be cleavage fractures with secondary cracks and micro-voids. The 13 Mn fracture morphology was a plastic fracture with many coarse dimples. Transverse cracks perpendicular to the tensile direction occurred on the surface of the gauge area of 5 Mn and 8 Mn tensile specimens, reducing plasticity dramatically. This was mainly related to the martensitic transformation produced by stress. We characterized the martensite near the tensile fracture and speculated the main mode of crack propagation. Furthermore, a little amount of sharp-shaped BCC phase was found in the 5 Mn, which was determined to be a hard phase relative to the austenite matrix by nanoindentation test. These steels have stacking fault energies ranging from ~15 to ~29 mJ/m2 with increasing Mn content 13 Mn has high stacking fault energy (SFE) and austenite stability. Twin-induced plasticity (TWIP) was the deformation mechanism.
Microstructure and Mechanical Properties of Magnesium Matrix Composites Reinforced by In Situ Reduced Graphene Oxide
Authors: Liu Feixiang , Wang Zhaohui , Du Xian , Li Shubo , Du Wenbo
Published: March 2023

Due to their excellent mechanical properties and large specific surface area, graphene and its derivatives are widely used in metal matrix composites as reinforcements. In this study, the thermal reduction behavior of large-size graphene oxide are investigated systematically, and reduced graphene oxide (RGO) with few residual oxygen groups and good structural integrity is obtained. ZK61 matrix composites with varying content of in situ RGO are fabricated using the semi-powder metallurgy method. The results reveal that the addition of RGO can cause the refinement of the grains and the second phase, which is attributed to the uniform distribution of the RGO throughout the matrix. The formation of nano-MgO particles is beneficial in increasing the interfacial bonding strength between the RGO and the matrix, resulting in simultaneous increments in yield strength and elongation in the RGO/ZK61 composites. The composite containing 0.6 wt.% RGO shows a superior mechanical property, including microhardness of 79.9 HV, yield strength of 203 MPa and excellent elongation of 17.5%, with increases of 20.9%, 8.6% and 7.4%, respectively, when compared with the ZK61 alloy. Quantitative analysis indicates that the main strengthening mechanisms of RGO-reinforced magnesium matrix composites are load transfer strengthening and grain refinement strengthening.
Structure and Physical Properties of Mg93−xZnxCa7 Metallic Glasses
Authors: Michalik Štefan , Molčanová Zuzana , Šulíková Michaela , Kušnírová Katarína , Jóvári Pál , Darpentigny Jacques , Saksl Karel
Published: March 2023

The Mg-Zn-Ca system has previously been proposed as the most suitable biodegradable candidate for biomedical applications. In this work, a series of ribbon specimens was fabricated using a melt-spinning technique to explore the glass-forming ability of the Mg-Zn-Ca system along the concentration line of 7 at.% of calcium. A glassy state is confirmed for Mg50Zn43Ca7, Mg60Zn33Ca7, and Mg70Zn23Ca7. Those samples were characterised by standard methods to determine their mass density, hardness, elastic modulus, and crystallisation temperatures during devitrification. Their amorphous structure is described by means of pair distribution functions obtained by high-energy X-ray and neutron diffraction (HEXRD and ND) measurements performed at large-scale facilities. The contributions of pairs Mg-Mg, Mg-Zn, and Zn-Zn were identified. In addition, a transformation process from an amorphous to crystalline structure is followed in situ by HEXRD for Mg60Zn33Ca7 and Mg50Zn43Ca7. Intermetallic compounds IM1 and IM3 and hcp-Mg phase are proposed to be formed in multiple crystallisation eventss.
Decorative Chromium Coatings on Polycarbonate Substrate for the Automotive Industry
Authors: Ponte Filipa , Sharma Pooja , Figueiredo Nuno Miguel , Ferreira Jorge , Carvalho Sandra
Published: March 2023

Metal-coated plastic parts are replacing traditional metallic materials in the automotive industry. Sputtering is an alternative technology that is more environmentally friendly than electrolytic coatings. Most metalized plastic parts are coated with a thin metal layer (~100–200 nm). In this work, the challenge is to achieve thicker films without cracking or without other defects, such as pinholes or pores. Chromium coatings with different thicknesses were deposited onto two different substrates, polycarbonate with and without a base coat, using dc magnetron sputtering in an atmosphere of Ar. Firstly, in order to improve the coating adhesion on the polymer surface, a plasma etching treatment was applied. The coatings were characterized for a wide thickness range from 800 nm to 1600 nm. As the thickness of the coatings increased, there was an increase in the specular reflectivity and roughness of the coatings and changes in morphology due to the columnar growth of the film and a progressive increase in thermal stresses. Furthermore, a decrease in the hardness and the number of pinholes was noticed. The maximum thickness achieved without forming buckling defects was 1400 nm. The tape tests confirmed that every deposited coating showed a good interface adhesion to both polymers.
Improved Crystallinity of Annealed 0002 AlN Films on Sapphire Substrate
Authors: Bersch Bruno Comis , Caminal Ros Tomàs , Tollefsen Vegard , Johannessen Erik Andrew , Johannessen Agne
Published: March 2023

AlN is a piezoelectric material used in telecommunication applications due to its high surface acoustic wave (SAW) velocity, stability, and mechanical strength. Its performance is linked to film quality, and one method to achieve high-quality films goes through the process of annealing. Consequently, c-orientated AlN film with a thickness of 1.1 μm deposited on sapphire was annealed at temperatures of 1100 °C and 1150 °C in a N2 controlled atmosphere. This was compared to annealing at 1100 °C, 1450 °C, and 1700 °C with N2 flow in an open atmosphere environment. Sample rotation studies revealed a significant impact on the ⍵-2θ X-ray rocking curve. A slight variation in the film crystallinity across the wafer was observed. After the annealing, it was found that the lattice parameter c was increased by up to 2%, whereas the screw dislocation density dropped from 3.31 × 1010 to 0.478 × 1010 cm−2, and the full width at half maximum (FWHM) of reflection (0002) was reduced from 1.16° to 0.41° at 1450 °C. It was shown that annealing in a N2-controlled atmosphere plays a major role in reducing the oxidation of the AlN film, which is important for acoustic wave devices where the electrodes are placed directly on the piezoelectric substrate. The face-to-face arrangement of the samples could further reduce this oxidation effect.
Optimal Design of Bubble Deck Concrete Slabs: Sensitivity Analysis and Numerical Homogenization
Authors: Staszak Natalia , Garbowski Tomasz , Ksit Barbara
Published: March 2023

The use of layered or hollow floors in the construction of buildings obviously reduces the self-weight of the slab, and their design requires some expertise. In the present work, a sensitivity analysis and numerical homogenization were used to select the most important characteristics of bubble deck floors that have a direct or indirect impact on their load capacity. From the extensive case study, conclusions were drawn regarding the optimal selection of geometry, materials, and the arrangement and size of air voids in such a way as to ensure high stiffness of the cross-section and at the same time maximally reduce the self-weight of the slabs. The conducted analyses showed that the height of the slab and the geometry of the voids had the greatest impact on the load-bearing capacity. The concrete class and reinforcement used are of secondary importance in the context of changes in load-bearing capacity. Both the type of steel and the amount of reinforcement has a rather small or negligible influence on the bubble deck stab stiffness. Of course, the geometry of the voids and their arrangement and shape have the greatest influence on the drop in the self-weight of the floor slabs. Based on the presented results of the sensitivity analysis combined with numerical homogenization, a set of the most important design parameters was ordered and selected for use in the optimization procedure.
Residual Stress Formation Mechanisms in Laser Powder Bed Fusion—A Numerical Evaluation
Authors: Kaess Moritz , Werz Martin , Weihe Stefan
Published: March 2023

Additive manufacturing methods, such as the laser powder bed fusion, do not need any special tool or casting mold. This enables the fast realization of complex and individual geometries with integrated functions. However, the local heat input during the manufacturing process often leads to residual stresses and distortion. This in turn causes poor quality, scrap parts or can even terminate a job prematurely if the powder recoating mechanism collides with a distorted part during the process. This study investigates the generation mechanisms of residual stresses and distortion during laser powder bed fusion (LPBF) of stainless steel 316L in order to reduce these effects and thus contribute to improved process safety and efficiency. Therefore, numerical investigations with a finite element model on the scale of a few melt tracks and layers serve to develop a detailed understanding of the mechanisms during production. The work includes an investigation of the build plate temperature, the laser power and speed and the layer thickness. The results show a strong dependency on the build plate preheating and energy per unit length. A higher build plate temperature and a reduction of the energy per unit length both lead to lower residual stresses.
Experimental Research on the Wear Behavior of Materials Used in the Manufacture of Components for Cement Concrete Mixers
Authors: Niță Adrian , Petrescu Marius Gabriel , Dumitru Teodor , Burlacu Andrei , Tănase Maria , Laudacescu Eugen , Ramadan Ibrahim
Published: March 2023

Mixers used in the production of cement concrete operate under special conditions such as mechanical stresses, abrasive–erosive friction phenomena, and corrosive working environments. In this paper, the authors aimed to establish a correlation between the chemical composition of mixer blade materials and their wear behavior. Three types of alloyed (chromium) cast iron were used for an experimental program that included three sets of tests in accelerated wear conditions which replicated the actual working environment (mixture of mineral aggregate, sand, cement, and water). The tribological tests were carried out using a Baroid tribometer. The results indicated that regardless of the test environment, cast iron with the highest chromium content exhibited the best wear resistance. However, it cannot be concluded that the wear resistance of the studied cast iron materials increases as a direct result of an increase in chromium content. For a chromium content of less than 25%, a better tribological behavior was observed for cast iron with a lower chromium content (of about 4%) than for cast irons with a higher chromium content (of about 9%).
Effect of Plasma Argon Pretreatment on the Surface Properties of AZ31 Magnesium Alloy
Authors: Montero Cecilia , Ramírez Cristián Gino , Muñoz Lisa , Sancy Mamié , Azócar Manuel , Flores Marcos , Artigas Alfredo , Zagal José H. , Zhou Xiaorong , Monsalve Alberto , Páez Maritza
Published: March 2023

Climate change has evidenced the need to reduce carbon dioxide emissions into the atmosphere, and so for transport applications, lighter weight alloys have been studied, such as magnesium alloys. However, they are susceptible to corrosion; therefore, surface treatments have been extensively studied. In this work, the influence of argon plasma pretreatment on the surface properties of an AZ31 magnesium alloy focus on the enhancement of the reactivity of the surface, which was examined by surface analysis techniques, electrochemical techniques, and gravimetric measurements. The samples were polished and exposed to argon plasma for two minutes in order to activate the surface. Contact angle measurements revealed higher surface energy after applying the pretreatment, and atomic force microscopy showed a roughness increase, while X-Ray photoelectron spectroscopy showed a chemical change on the surface, where after pretreatment the oxygen species increased. Electrochemical measurements showed that surface pretreatment does not affect the corrosion mechanism of the alloy, while electrochemical impedance spectroscopy reveals an increase in the original thickness of the surface film. This increase is likely associated with the high reactivity that the plasma pretreatment confers to the surface of the AZ31 alloy, affecting the extent of oxide formation and, consequently, the increase in its protection capacity. The weight loss measurements support the effect of the plasma pretreatment on the oxide thickness since the corrosion rate of the pretreated AZ31 specimens was lower than that of those that did not receive the surface pretreatment.
Pyrolysis and Oxidative Thermal Decomposition Investigations of Tennis Ball Rubber Wastes through Kinetic and Thermodynamic Evaluations
Authors: Wan Hai-Bo , Huang Zhen
Published: March 2023

Thermal decomposition of tennis ball rubber (TBR) wastes in nitrogen and air has been studied through thermogravimetric analysis. The samples were thermally decomposed from room temperature to 950 K at heating rates of 3 to 20 K/min with a purging flow of 30 cm3/min. The degradation features and specific temperatures for two purging gases are thus compared according to the nonisothermal results. Kinetic analyses of two thermal decomposition processes have been isoconversionally performed using differential or integral methods. The activation energy as a function of mass conversion has been thus obtained over the entire decomposition range, varying from 116.7 to 723.3 kJ/mol for pyrolysis and 98.2 to 383.6 kJ/mol for oxidative thermal decomposition. The iterative Flynn–Wall–Ozawa method combined with the linear compensation effect relationship has been proposed for determining the pre-exponential factor and reaction mechanism function, resulting in chemical order reaction models of f(α) = (1 − α)5.7 and f(α) = (1 − α)5.8 for describing pyrolysis and the oxidative thermal degradation of TBR wastes, respectively. With these kinetic parameters, very satisfactory matching against experimental data has been obtained for both gases. Additionally, the thermodynamic parameters, such as the changes of entropy, enthalpy and Gibbs free energy, over the whole thermal degradation processes have also been evaluated.
Preparation of In Situ Growth Multiscale β-Sialon Grain-Reinforced Al2O3-Based Composite Ceramic Tool Materials
Authors: Zhu Jian , Xue Yunna , Bai Xiaolan , Shen Xuehui , He Jianqun , Zhang Yu , Li Anhai
Published: March 2023

A kind of multiscale β-sialon grain-reinforced Al2O3 matrix composite ceramic tool material, named ASN, was prepared and studied. For the ASN, β-sialon (molecular formula: Si4Al2O2N6) was synthesized in situ by a hot-pressing and solid-solution reaction process. A total of six samples were prepared at varying sintering temperatures and holding times under vacuum conditions. The solid solution reaction mechanism of β-sialon, the phase composition, mechanical properties, microstructure, and strengthening and toughening mechanisms of the composite ASN were investigated. As a result, within the experimental parameters, an optimal ASN tool material was obtained under a pressure of 32 MPa and at a temperature of 1550 °C for 20 min. The tested mechanical properties of the optimal sample were as follows: flexural strength 997 ± 59 MPa, fracture toughness 6.4 ± 0.3 MPa·m1/2, Vickers hardness 18.2 ± 0.4 GPa, and relative density 98.1 ± 0.2%. According to crystal defect theory, the solid solution reaction mechanism of in-situ-synthesized β-sialon in an Al2O3 matrix involves a double mechanism of unequivalence (or hetero-valence) and interstitial filling. The multiscale β-sialon grains mainly consisted of four grains, which were elongated β-sialon grains with a diameter of 0.3–0.4 μm and an aspect ratio of 6–9, elongated β-sialon grains with a diameter of 70 nm and an aspect ratio of 10, β-sialon whiskers with a diameter of 0.2 μm and an aspect ratio of 12–15, and intragranular β-sialon whiskers with a diameter of 70 nm. The mechanical properties were improved due to strengthening and toughening mechanisms, such as mixed structure mode (intergranular and transgranular), elongated grain pullout, interface bonding, crack reflection, pinning, and bridging.
Application of Virtual Reality Systems in Bone Trauma Procedures
Authors: Ugwoke Chiedozie Kenneth , Albano Domenico , Umek Nejc , Dumić-Čule Ivo , Snoj Žiga
Published: March 2023

Background and Objectives: Bone fractures contribute significantly to the global disease and disability burden and are associated with a high and escalating incidence and tremendous economic consequences. The increasingly challenging climate of orthopaedic training and practice re-echoes the established potential of leveraging computer-based reality technologies to support patient-specific simulations for procedural teaching and surgical precision. Unfortunately, despite the recognised potential of virtual reality technologies in orthopaedic surgery, its adoption and integration, particularly in fracture procedures, have lagged behind other surgical specialities. We aimed to review the available virtual reality systems adapted for orthopaedic trauma procedures. Materials and Methods: We performed an extensive literature search in Medline (PubMed), Science Direct, SpringerLink, and Google Scholar and presented a narrative synthesis of the state of the art on virtual reality systems for bone trauma procedures. Results: We categorised existing simulation modalities into those for fracture fixation techniques, drilling procedures, and prosthetic design and implantation and described the important technical features, as well as their clinical validity and applications. Conclusions: Over the past decade, an increasing number of high- and low-fidelity virtual reality systems for bone trauma procedures have been introduced, demonstrating important benefits with regard to improving procedural teaching and learning, preoperative planning and rehearsal, intraoperative precision and efficiency, and postoperative outcomes. However, further technical developments in line with industry benchmarks and metrics are needed in addition to more standardised and rigorous clinical validation.
In Situ Measurement of Spindle Radial Error for Ultra-Precision Machining Based on Three-Point Method
Authors: Xu Hanwei , Sun Zizhou , Dai Yifan , Guan Chaoliang , Hu Hao , Wang Yu
Published: March 2023

The radial error is an important parameter to evaluate the performance of ultra-precision spindles. The three-point method has not yet been well applied in nanometer-scale measurement due to its disadvantages of harmonic suppression and the complicated error separation process. In order to verify that the three-point method can realize the nanometer-scale measurement of the radial error in the machining environment, an in situ measurement and evaluation system is established. Experiments are performed using the system, and a comparative experiment is conducted to verify the accuracy of the system. The average value and standard deviation of the measurement results are 23.096 nm and 0.556 nm, respectively. The in situ measurement result was in good agreement with the Donaldson reversal method using a commercially available spindle analyzer.
Numerical and Experimental Study on the Colliding Flow Pulp Conditioning for the Separation Intensification of Unburned Carbon from Coal Gasification Slag
Authors: Chen Ruifeng , Wang Hainan , Li Danlong , Liao Yinfei , Tian Quanzhi , Su Wanli , Li Lei , Zhang Haijun
Published: March 2023

As a byproduct of the coal gasification process, a large amount of coal gasification slag is generated. The failure to fully dispose of it has caused the occupation of land resources and environmental pollution. Before its comprehensive utilization, the carbon and ash constituents must be separated, for which flotation is an effective method. However, the small difference in surface hydrophobicity of them cannot result in a high-efficiency separation. Therefore, a colliding flow pulp conditioning device (CFPCD) was proposed in this work to improve the interaction between the collector droplets and fine particles, and strengthen the modification of collector on the particle surface by generating a properly constructed turbulent flow field. Computational fluid dynamics (CFD) was employed to simulate the internal flow field of CFPCD to obtain the critical flow field parameters, such as the velocity, strain rate, turbulent kinetic energy, turbulent dissipation rate, and turbulent eddy scale. Additionally, particle wrap angle measurements and flotation tests were conducted to verify the performance of pulp conditioning. The results showed that a velocity gradient was obvious in the inner cylinder colliding flow area, thereby inducing the large strain rate and the intense turbulence, which were responsible for the pulp homogenization and the enhanced particle-collector interaction. With the feeding velocity increased, the fluid shear was larger and the improved performance was more obvious. According to the flotation results, the maximum recovery of unburned carbon was obtained with the feeding velocity equal to 2.5 m/s, which was consistent with the tendency of wrap angle. Meanwhile, the loss on ignition of the tailings reached the optimal value, corresponding to 9.94%.
Nutritional Assessment of the Symptomatic Patient on a Plant-Based Diet: Seven Key Questions
Authors: Plotnikoff Gregory A. , Dobberstein Linda , Raatz Susan
Published: March 2023

Plant-based diets, both vegan and vegetarian, which emphasize grains, vegetables, fruits, legumes, nuts, and seeds are increasingly popular for health as well as financial, ethical, and religious reasons. The medical literature clearly demonstrates that whole food plant-based diets can be both nutritionally sufficient and medically beneficial. However, any person on an intentionally restrictive, but poorly-designed diet may predispose themselves to clinically-relevant nutritional deficiencies. For persons on a poorly-designed plant-based diet, deficiencies are possible in both macronutrients (protein, essential fatty acids) and micronutrients (vitamin B12, iron, calcium, zinc, and vitamin D). Practitioner evaluation of symptomatic patients on a plant-based diet requires special consideration of seven key nutrient concerns for plant-based diets. This article translates these concerns into seven practical questions that all practitioners can introduce into their patient assessments and clinical reasoning. Ideally, persons on plant-based diets should be able to answer these seven questions. Each serves as a heuristic prompt for both clinician and patient attentiveness to a complete diet. As such, these seven questions support increased patient nutrition knowledge and practitioner capacity to counsel, refer, and appropriately focus clinical resources.
Systematic Review and Dose-Response Meta-Analysis on the Relationship between Different Gluten Doses and Risk of Coeliac Disease Relapse
Authors: Rostami-Nejad Mohammad , Asri Nastaran , Olfatifar Meysam , Khorsand Babak , Houri Hamidreza , Rostami Kamran
Published: March 2023

Gluten proteins are known as immunological triggers for inflammation resulting in mucosal lesions in patients with coeliac disease (CD). Adherence to a strict gluten-free diet (GFD) is currently known as the only effective treatment for CD. In this study, we performed a systematic review and dose-response meta-analysis on data from previous studies to investigate the association between different gluten doses administered and the risk of CD relapse. Electronic databases were systematically searched to retrieve studies that investigated the response of CD patients to different amounts of gluten intake and evaluated the clinical, serologic, and/or histologic evidence to recognize disease relapse. Study-specific relative risks (RRs) were combined using a random effects model. A total of 440 identified published papers were screened, of which 7 records were selected following full-text reviewing and eligibility assessment for dose-response meta-analysis. According to our analysis, the risk of CD relapse is estimated to be 0.2% (RR: 1.002; 95% CI: 1.001 to 1.004) following the consumption of 6 mg gluten/day, which was increased to 7% (RR: 1.07; 95% CI: 1.03 to 1.10), 50% (RR: 1.50; 95% CI: 1.23 to 1.82), 80% (RR: 1.80; 95% CI: 1.36 to 2.38), and 100% (RR: 2.00; 95% CI: 1.43 to 2.78) by the daily intake of 150, 881, 1276, and 1505 mg gluten, respectively. Although good adherence to a GFD can adequately control CD-related symptoms, disease relapse might happen even with a very low dose of gluten, and the duration of exposure to gluten is also an important matter. The current literature has substantial limitations, such as relying on the data from just a few countries that were different in terms of the amount of gluten administered, the duration of the challenge, etc. Therefore, more randomized clinical trials using a standardized gluten challenge protocol are needed to confirm the findings of the present study.
Dietary Trehalose as a Bioactive Nutrient
Authors: Chen Anqi , Gibney Patrick A.
Published: March 2023

Trehalose is a naturally occurring, non-reducing disaccharide comprising two covalently-linked glucose molecules. It possesses unique physiochemical properties, which account for multiple biological roles in a variety of prokaryotic and eukaryotic organisms. In the past few decades, intensive research on trehalose has uncovered its functions, and extended its uses as a sweetener and stabilizer in the food, medical, pharmaceutical, and cosmetic industries. Further, increased dietary trehalose consumption has sparked research on how trehalose affects the gut microbiome. In addition to its role as a dietary sugar, trehalose has gained attention for its ability to modulate glucose homeostasis, and potentially as a therapeutic agent for diabetes. This review discusses the bioactive effects of dietary trehalose, highlighting its promise in future industrial and scientific contributions.
How Does Dietary Intake Relate to Dispositional Optimism and Health-Related Quality of Life in Germline BRCA1/2 Mutation Carriers?
Authors: Esser Anne , Neirich Leonie , Grill Sabine , Bischoff Stephan C. , Halle Martin , Siniatchkin Michael , Yahiaoui-Doktor Maryam , Kiechle Marion , Lammert Jacqueline
Published: March 2023

Background: The Mediterranean diet (MD) is an anti-inflammatory diet linked to improved health-related quality of life (HRQoL). Germline (g)BRCA1/2 mutation carriers have an increased risk of developing breast cancer and are often exposed to severe cancer treatments, thus the improvement of HRQoL is important. Little is known about the associations between dietary intake and HRQoL in this population. Methods: We included 312 gBRCA1/2 mutation carriers from an ongoing prospective randomized controlled lifestyle intervention trial. Baseline data from the EPIC food frequency questionnaire was used to calculate the dietary inflammatory index (DII), and adherence to MD was captured by the 14-item PREDIMED questionnaire. HRQoL was measured by the EORTC QLQ-C30 and LOT-R questionnaires. The presence of metabolic syndrome (MetS) was determined using anthropometric measurements, blood samples and vital parameters. Linear and logistic regression models were performed to assess the possible impact of diet and metabolic syndrome on HRQoL. Results: Women with a prior history of cancer (59.6%) reported lower DIIs than women without it (p = 0.011). A greater adherence to MD was associated with lower DII scores (p < 0.001) and reduced odds for metabolic syndrome (MetS) (p = 0.024). Women with a more optimistic outlook on life reported greater adherence to MD (p < 0.001), whereas a more pessimistic outlook on life increased the odds for MetS (OR = 1.15; p = 0.023). Conclusions: This is the first study in gBRCA1/2 mutation carriers that has linked MD, DII, and MetS to HRQoL. The long-term clinical implications of these findings are yet to be determined.
Lyophilization for Formulation Optimization of Drug-Loaded Thermoresponsive Polyelectrolyte Complex Nanogels from Functionalized Hyaluronic Acid
Authors: Le Huu Van , Dulong Virginie , Picton Luc , Le Cerf Didier
Published: March 2023

The lyophilization of nanogels is practical not only for their long-term conservation but also for adjusting their concentration and dispersant type during reconstitution for different applications. However, lyophilization strategies must be adapted to each kind of nanoformulation in order to minimize aggregation after reconstitution. In this work, the effects of formulation aspects (i.e., charge ratio, polymer concentration, thermoresponsive grafts, polycation type, cryoprotectant type, and concentration) on particle integrity after lyophilization and reconstitution for different types of polyelectrolyte complex nanogels (PEC-NGs) from hyaluronic acid (HA) were investigated. The main objective was to find the best approach for freeze-drying thermoresponsive PEC-NGs from Jeffamine-M-2005-functionalized HA, which has recently been developed as a potential platform for drug delivery. It was found that freeze-drying PEC-NG suspensions prepared at a relatively low polymer concentration of 0.2 g.L−1 with 0.2% (m/v) trehalose as a cryoprotectant allow the homogeneous redispersion of PEC-NGs when concentrated at 1 g.L−1 upon reconstitution in PBS without important aggregation (i.e., average particle size remaining under 350 nm), which could be applied to concentrate curcumin (CUR)-loaded PEC-NGs for optimizing CUR content. The thermoresponsive release of CUR from such concentrated PEC-NGs was also reverified, which showed a minor effect of freeze-drying on the drug release profile.
Real-Time Monitoring of Colorectal Cancer Location and Lymph Node Metastasis and Photodynamic Therapy Using Fucoidan-Based Therapeutic Nanogel and Near-Infrared Fluorescence Diagnostic–Therapy System
Authors: Shin Yoo-kyoung , Park You-rim , Lee Hyeri , Choi Yongdoo , Eom Joo Beom
Published: March 2023

We report real-time monitoring of colorectal cancer, lymph node metastasis of colorectal cancer cells, and tumor growth inhibition through photodynamic therapy (PDT) using a near-infrared fluorescence diagnostic–therapy system with a light source for PDT and a fucoidan-based theranostic nanogel (CFN-gel) with good accumulation efficiency in cancer cells. To confirm the effect of the fabricated system and developed CFN-gel, in vitro and in vivo experiments were performed. Chlorin e6 (Ce6) and 5-aminolevulinic acid (5-ALA) were used for comparison. We confirmed that CFN-gel has a high accumulation efficiency in cancer cells and high fluorescence signals in near-infrared light for a long period, and only CFN-gel delayed the growth rate of cancer in terms of its size in PDT. In addition, using the near-infrared fluorescence diagnostic–therapy system and CFN-gel prepared for these experiments, the lymph node metastasis of cancer cells was imaged in real time, and the metastasis was confirmed through H&E staining. The possibility of image-guided surgery and identification of lymph node metastasis in colorectal cancer can be confirmed through CFN-gel and a near-infrared fluorescence diagnostic–therapy system that includes various light sources.
Utilization of Functionalized Metal–Organic Framework Nanoparticle as Targeted Drug Delivery System for Cancer Therapy
Authors: Tran Vy Anh , Thuan Le Van , Doan Van Dat , Vo Giang N. L.
Published: March 2023

Cancer is a multifaceted disease that results from the complex interaction between genetic and environmental factors. Cancer is a mortal disease with the biggest clinical, societal, and economic burden. Research on better methods of the detection, diagnosis, and treatment of cancer is crucial. Recent advancements in material science have led to the development of metal–organic frameworks, also known as MOFs. MOFs have recently been established as promising and adaptable delivery platforms and target vehicles for cancer therapy. These MOFs have been constructed in a fashion that offers them the capability of drug release that is stimuli-responsive. This feature has the potential to be exploited for cancer therapy that is externally led. This review presents an in-depth summary of the research that has been conducted to date in the field of MOF-based nanoplatforms for cancer therapeutics.
Half a Century of Fragmented Research on Deviations from Advised Therapies: Is This a Good Time to Call for Multidisciplinary Medication Adherence Research Centres of Excellence?
Authors: Kardas Przemysław , Ágh Tamás , Dima Alexandra , Goetzinger Catherine , Potočnjak Ines , Wettermark Björn , van Boven Job F. M.
Published: March 2023

Medication adherence is a key precondition of the effectiveness of evidence-based therapies. However, in real-life settings, non-adherence to medication is still very common. This leads to profound health and economic consequences at both individual and public health levels. The problem of non-adherence has been extensively studied in the last 50 years. Unfortunately, with more than 130,000 scientific papers published on that subject so far, we are still far from finding an ultimate solution. This is, at least partly, due to fragmented and poor-quality research that has been conducted in this field sometimes. To overcome this deadlock, there is a need to stimulate the adoption of best practices in medication adherence-related research in a systematic way. Therefore, herein we propose the establishment of dedicated medication adherence research Centres of Excellence (CoEs). These Centres could not only conduct research but could also create a profound societal impact, directly serving the needs of patients, healthcare providers, systems and economies. Additionally, they could play a role as local advocates for good practices and education. In this paper, we propose some practical steps that might be taken in order to establish such CoEs. We describe two success stories, i.e., Dutch and Polish Medication Adherence Research CoEs. The COST Action “European Network to Advance Best practices & technoLogy on medication adherencE” (ENABLE) aims to develop a detailed definition of the Medication Adherence Research CoE in the form of a list of minimal requirements regarding their objectives, structure and activities. We hope that it will help to create a critical mass and catalyse the setup of regional and national Medication Adherence Research CoEs in the near future. This, in turn, may not only increase the quality of the research but also raise the awareness of non-adherence and promote the adoption of the best medication adherence-enhancing interventions.
Physiologically Based Pharmacokinetic Modelling to Predict Pharmacokinetics of Enavogliflozin, a Sodium-Dependent Glucose Transporter 2 Inhibitor, in Humans
Authors: Kim Min-Soo , Song Yoo-Kyung , Choi Ji-Soo , Ji Hye Young , Yang Eunsuk , Park Joon Seok , Kim Hyung Sik , Kim Min-Joo , Cho In-Kyung , Chung Suk-Jae , Chae Yoon-Jee , Lee Kyeong-Ryoon
Published: March 2023

Enavogliflozin is a sodium-dependent glucose cotransporter 2 (SGLT2) inhibitor approved for clinical use in South Korea. As SGLT2 inhibitors are a treatment option for patients with diabetes, enavogliflozin is expected to be prescribed in various populations. Physiologically based pharmacokinetic (PBPK) modelling can rationally predict the concentration–time profiles under altered physiological conditions. In previous studies, one of the metabolites (M1) appeared to have a metabolic ratio between 0.20 and 0.25. In this study, PBPK models for enavogliflozin and M1 were developed using published clinical trial data. The PBPK model for enavogliflozin incorporated a non-linear urinary excretion in a mechanistically arranged kidney model and a non-linear formation of M1 in the liver. The PBPK model was evaluated, and the simulated pharmacokinetic characteristics were in a two-fold range from those of the observations. The pharmacokinetic parameters of enavogliflozin were predicted using the PBPK model under pathophysiological conditions. PBPK models for enavogliflozin and M1 were developed and validated, and they seemed useful for logical prediction.
Facile Fabrication of High-Performance Composite Films Comprising Polyvinyl Alcohol as Matrix and Phenolic Tree Extracts
Authors: Xu Ying , Liu Bowen , Zheng Lulu , Zhou Yunxia , Essawy Hisham , Chen Xinyi , Zhou Xiaojian , Zhang Jun
Published: March 2023

Given that tree extracts such as tannin and lignin can be efficiently used as modifying materials, this helps to verify the global trend of energy saving and environment protection. Thus, bio-based biodegradable composite film incorporating tannin and lignin as additives, together with polyvinyl alcohol (PVOH) as a matrix (denoted TLP), was prepared. Its easy preparation process endows it with high industrial value in comparison to some bio-based films with complex preparation process such as cellulose-based films. Furthermore, imaging with scanning electron microscopy (SEM) shows that the surface of tannin- and lignin-modified polyvinyl alcohol film was smooth, free of pores or cracks. Moreover, the addition of lignin and tannin improved the tensile strength of the film, which reached 31.3 MPa as indicated by mechanical characterization. This was accounted for by using Fourier transform infrared (FTIR) and electrospray ionization mass (ESI-MS) spectroscopy, which showed that the physical blending of lignin and tannin with PVOH was accompanied by chemical interactions that gave rise to weakening of the prevailing hydrogen bonding in PVOH film. In consequence, the addition of tannin and lignin acquired the composite film good resistance against the light in the ultraviolet and visible range (UV-VL). Furthermore, the film exhibited biodegradability with a mass loss about 4.22% when contaminated with Penicillium sp. for 12 days.
Nonlinear Mechanical Property of 3D Braided Composites with Multi-Types Micro-Distortion: A Quantitative Evaluation
Authors: Zhai Junjun , Kong Xiangxia , Wang Luchen , Yan Shi , Jiang Lili , Cai Zhiwei
Published: March 2023

A new alternative calculation procedure is developed to quantify the effect of yarn distortion characteristics on the mechanical properties of three-dimensional (3D) braided carbon/resin composites. Firstly, the multi-type yarn distortion characteristics factors including path, cross-section shape and cross-section torsion effects are described based on the stochastic theory. Then, the multiphase finite element method is employed to overcome the complex discretization in traditional numerical analysis, and the parametric studies including multi-type yarn distortion and different braided geometrical parameters on the resulting mechanical properties are performed. It is shown that the proposed procedure can simultaneously capture the yarn path and cross-section distortion characteristics caused by the mutual squeeze of component materials, which is difficult to characterize by experimental methods. In addition, it is found that even small distortions of yarn may significantly affect the mechanical properties for 3D braided composites, and the 3D braided composites with different braiding geometric parameters will show different sensitivity to the distortion characteristics factors of yarn. The procedure, which could be implemented into commercial finite element codes, is an efficient tool for the design and structural optimization analysis of a heterogeneous material with anisotropic properties or complex geometries.
Exploring the Antimicrobial and Antitumoral Activities of Naphthoquinone-Grafted Chitosans
Authors: Pauli Fernanda Petzold , Freitas Cyntia Silva , Pereira Patricia Ribeiro , Magalhães Alviclér , de Carvalho da Silva Fernando , Paschoalin Vania M. F. , Ferreira Vitor Francisco
Published: March 2023

Biopolymers obtained from natural macromolecules are noteworthy among materials presenting high biocompatibility and adequate biodegradability, as is the case of chitosan (CS), making this biopolymeric compound a suitable drug delivery system. Herein, chemically-modified CS were synthetized using 2,3-dichloro-1,4-naphthoquinone (1,4-NQ) and the sodium salt of 1,2-naphthoquinone-4-sulfonic acid (1,2-NQ), producing 1,4-NQ-CS and 1,2-NQ-CS by three different methods, employing an ethanol and water mixture (EtOH:H2O), EtOH:H2O plus triethylamine and dimethylformamide. The highest substitution degree (SD) of 0.12 was achieved using water/ethanol and triethylamine as the base for 1,4-NQ-CS and 0.54 for 1,2-NQ-CS. All synthesized products were characterized by FTIR, elemental analysis, SEM, TGA, DSC, Raman, and solid-state NMR, confirming the CS modification with 1,4-NQ and 1,2-NQ. Chitosan grafting to 1,4-NQ displayed superior antimicrobial activities against Staphylococcus aureus and Staphylococcus epidermidis associated with improved cytotoxicity and efficacy, indicated by high therapeutic indices, ensuring safe application to human tissue. Although 1,4-NQ-CS inhibited the growth of human mammary adenocarcinoma cells (MDA-MB-231), it is accompanied by cytotoxicity and should be considered with caution. The findings reported herein emphasize that 1,4-NQ-grafted CS may be useful in protecting injured tissue against bacteria, commonly found in skin infections, until complete tissue recovery.
Rheological Behavior and Printability Study of Tri-Calcium Phosphate Ceramic Inks for Direct Ink Writing Method
Authors: Paul D L Belgin , Praveen Ayyappan Susila , Čepová Lenka , Elangovan Muniyandy
Published: March 2023

In the biomedical industry, tricalcium phosphate is a bioceramic substance that is frequently employed in the fabrication of scaffolds and bone structures. Fabrication of porous ceramic structures using conventional manufacturing techniques is very challenging because of the brittle nature of the ceramics, which has led to a newly adapted direct ink writing additive manufacturing method. This work investigates the rheology and extrudability of TCP inks to produce near-net-shape structures. Viscosity and extrudability tests found that stable TCP: Pluronic ink of 50 vol.% was more reliable compared to other tested inks prepared from a functional polymer group polyvinyl alcohol. A line study was carried out to identify the printing parameters suitable for printing structures from the selected ink with lesser dimensional error. Printing speed 5 mm/s and extrusion pressure 3 bar was found suitable to print a scaffold through a nozzle of 0.6 mm, keeping the stand-off distance equal to the nozzle diameter. The printed scaffold was further investigated for its physical and morphological structure of the green body. A suitable drying behavior was studied to remove the green body without cracking and wrapping before the sintering of the scaffold.
Fine Tuning of the Mechanical Properties of Bio-Based PHB/Nanofibrillated Cellulose Biocomposites to Prevent Implant Failure Due to the Bone/Implant Stress Shielding Effect
Authors: Ferri Martina , Chiromito Emanoele Maria Santos , de Carvalho Antonio Jose Felix , Morselli Davide , Degli Esposti Micaela , Fabbri Paola
Published: March 2023

A significant mechanical properties mismatch between natural bone and the material forming the orthopedic implant device can lead to its failure due to the inhomogeneous loads distribution, resulting in less dense and more fragile bone tissue (known as the stress shielding effect). The addition of nanofibrillated cellulose (NFC) to biocompatible and bioresorbable poly(3-hydroxybutyrate) (PHB) is proposed in order to tailor the PHB mechanical properties to different bone types. Specifically, the proposed approach offers an effective strategy to develop a supporting material, suitable for bone tissue regeneration, where stiffness, mechanical strength, hardness, and impact resistance can be tuned. The desired homogeneous blend formation and fine-tuning of PHB mechanical properties have been achieved thanks to the specific design and synthesis of a PHB/PEG diblock copolymer that is able to compatibilize the two compounds. Moreover, the typical high hydrophobicity of PHB is significantly reduced when NFC is added in presence of the developed diblock copolymer, thus creating a potential cue for supporting bone tissue growth. Hence, the presented outcomes contribute to the medical community development by translating the research results into clinical practice for designing bio-based materials for prosthetic devices.
Immobilization and Release of Platelet-Rich Plasma from Modified Nanofibers Studied by Advanced X-ray Photoelectron Spectroscopy Analyses
Authors: Manakhov Anton M. , Permyakova Elizaveta S. , Solovieva Anastasiya O. , Sitnikova Natalya A. , Kiryukhantsev-Korneev Philipp V. , Konopatsky Anton S. , Shtansky Dmitry V.
Published: March 2023

Platelet-rich Plasma (PRP) is an ensemble of growth factors, extracellular matrix components, and proteoglycans that are naturally balanced in the human body. In this study, the immobilization and release of PRP component nanofiber surfaces modified by plasma treatment in a gas discharge have been investigated for the first time. The plasma-treated polycaprolactone (PCL) nanofibers were utilized as substrates for the immobilization of PRP, and the amount of PRP immobilized was assessed by fitting a specific X-ray Photoelectron Spectroscopy (XPS) curve to the elemental composition changes. The release of PRP was then revealed by measuring the XPS after soaking nanofibers containing immobilized PRP in buffers of varying pHs (4.8; 7.4; 8.1). Our investigations have proven that the immobilized PRP would continue to cover approximately fifty percent of the surface after eight days.
Application of Amyloid-Based Hybrid Membranes in Drug Delivery
Authors: Lai You-Ren , Wang Steven S.-S. , Hsu Ti-Lun , Chou Szu-Hui , How Su-Chun , Lin Ta-Hsien
Published: March 2023

The properties of amyloid fibrils, e.g., unique structural characteristics and superior biocompatibility, make them a promising vehicle for drug delivery. Here, carboxymethyl cellulose (CMC) and whey protein isolate amyloid fibril (WPI-AF) were used to synthesize amyloid-based hybrid membranes as vehicles for the delivery of cationic and hydrophobic drugs (e.g., methylene blue (MB) and riboflavin (RF)). The CMC/WPI-AF membranes were synthesized via chemical crosslinking coupled with phase inversion. The zeta potential and scanning electron microscopy results revealed a negative charge and a pleated surface microstructure with a high content of WPI-AF. FTIR analysis showed that the CMC and WPI-AF were cross-linked via glutaraldehyde and the interacting forces between membrane and MB or RF was found to be electrostatic interaction and hydrogen bonding, respectively. Next, the in vitro drug release from membranes was monitored using UV-vis spectrophotometry. Additionally, two empirical models were used to analyze the drug release data and relevant rate constant and parameters were determined accordingly. Moreover, our results indicated that in vitro drug release rates depended on the drug–matrix interactions and transport mechanism, which could be controlled by altering the WPI-AF content in membrane. This research provides an excellent example of utilizing two-dimensional amyloid-based materials for drug delivery.