bluuebunny/crossref_metadata_2025 huggingface dataset:
Monitoring of chemotherapy successfulness of Platina/Taxol chemotherapy protocol by using determination of serum urokinase plasminogen activator (uPA) and soluble urokinase plasminogen activator receptor (suPAR) in patients with ovarian carcinoma FIGO II
Authors: Ljuca Dženita , Fatušić Zlatan , Iljazović Ermina , Ahmetović Begzudin
Published: February 2018

In about 70% of cases, ovarian carcinoma has been diagnosed at an advanced stage. Invasion and metastasis of solid tumors request protease activity resulting in basal membrane destruction and surrounding matrix. In that process, urokinase plasminogen activator (uPA) and its receptor, urokinase plasminogen activator receptor (suPAR) play a key role, that via plasmin activation lead to basal membrane and matrix degradation in surrounding of the tumor, enable to its invasion and metastasis. Determination of serum concentration of those tumor markers can be useful in preoperative as well as in postoperative period. Their serum concentrations in ovarian cancer patients may help in good monitoring of remission or progression during chemotherapy treatment. In late 1950s and eariy 1960s, when it was found out that malignant ovarian tumors were chemosensitive, their chemotherapy treatment has begun. In the beginning it was used only mono-therapy, and by discovering new cytostatics it was replaced by poly-chemotherapy. Now days, in the therapy of advanced stages of ovarian carcinoma combination of cisplatine or carboplatine with paclitaxel is considering as standard treatment. Aim of this study was to determine serum uPA, suPAR and CEA in FIGO II and III patients with different histo-logical type (serous, mucinous, clear cell tumor) before and after PT chemotherapy protocol during following three cycles. In this prospective study we have analyzed 17 patients with ovarian carcinoma, those have been after surgery treated by chemotherapy. Serum levels of uPA and suPAR have been determined by ELISA-test (Imubind uPA, Imubind uPAR, American Diagnostica), and CEA by OPUS Imunoassay method. Results of this study have shown that uPA, suPAR and CEA met criteria for prognostic markers for monitoring of successful-ness of platina/taxol chemotherapy protocol for serous, mucinous and clear cell tumor FIGO II and III stage of ovarian carcinoma. In case of PT chemotherapy protocol suPAR was better prognostic marker for monitoring of chemotherapy successfulness (Pearson coefficient 0,9 do 1,0; p<0,00l) than uPA (Pearson coefficient between 0,86 and 0,92; p<0,02) and CEA (Pearson coefficient 0,5 do 0,89; p<0,04).
Total Content of Phenols and Anthocyanins in Edible Fruits from Bosnia
Authors: Rimpapa Zlatan , Toromanovic Jasmin , Tahirovic Ismet , Šapčanin Aida , Sofic Emin
Published: February 2018

Content of total phenols and total anthocyanins was estimated in edible fruits from Bosnia by photometric methods. Cyanidin-3-galactoside chloride was used as a standard for determination of total anthocyanins, and galic acid served as a standard for determination of total phenols. Total content of phenols was 12.7 mg/g in elderberry fruits, 10.4 mg/g in bilberry, 9.8 mg/g in blackberry, 8.8 mg/g in wild cherry, 6.1 mg/g in cultivated blackberry, 3.5 mg/g in cultivated strawberry, 2.4 mg/g in average in sour cherry fruits from different locations and the lowest quantity of total phenols was in edible parts of melon, only 0.2 mg/g. Total content of anthocyanins was 6.8 mg/g in wild cherry, 6.7 mg/g in elderberry fruits and 4.5 mg/g in bilberry. Wild bilberry fruits from different locations had in average 3.5 mg/g, cherries from different locations 1.3 mg/g, cultivated blackberries 1.0 mg/g, cultivated strawberries 0.8 mg/ g while melon fruit had no anthocyanins at all. Acidity was measured in macerate of edible fruits by direct insertion of electrode. pH values in the macerates were as follows: 3.03 in bilberry, 3.45 in blackberries, 3.59 in sour cherries, 3.92 in wild cherries, 4.44 in elderberries and 6.19 in melon.
Hyperalgesia-Type Response Reveals No Difference in Pain-Related Behavior Between Wistar and Sprague-Dawley Rats
Authors: Vukojević Katarina , Lovrić-Kojundžić Sanja , Sapunar Damir
Published: February 2018

The experience of pain is variable among certain cultures, ethnical groups and among individuals. This variability can be explained by environmental influence, genetic predisposition and plasticity of the existing neuronal pathways. The purpose of this study was to examine a strain-related difference in pain sensitivity between Wistar and Sprague-Dawley rats strains and if there was a difference, could it be overcomes with the robust test. Mechanical sensitivity e.g. existence of paw withdrawal and complex hyperalgesia-type response after needle stimuli has been measured. Both hindpaws (middle, medial and lateral part) were stimulated randomly in appropriate intervals. The results did not demonstrate statistically significant strain difference in pain sensitivity, except in the lateral part of the hindpaw where Sprague-Dawley rats were more sensitive. This data emphasize the importance of selecting a robust behavior test that will be used in investigation of peripheral nerve injury and in neuropathic pain research.
Acute Mastoiditis in Children: Susceptibility Factors and Management
Authors: Spremo Slobodan , Udovčić Biljana
Published: February 2018

The objective was to review our experience with clinical course, diagnostic and therapeutic profile of children treated for acute mastoiditis, and to investigate for possible susceptibility factors. Study was designed as retrospective review of pediatric patients presenting with acute mastoiditis secondary to acute otitis media over the last 6 years, from 2000 to 2006. The study involved children aged from 1 to 16 years treated for acute mastoiditis and subsequent intratemporal and intracranial complications in Clinic for otorhinolaryngology, Clinic Center Banja Luka. Selected clinical parameters, mastoid coalescence and risk factors for necessity of surgical intervention were analyzed. Medical history review of a total of 13 patients with acute mastoiditis was analyzed. Acute coalescent mastoiditis occurred 11 patients (84%) while noncoalescent form of acute mastoiditis occurred in 2 cases (16%). Intracranial complication occurred in 3 patients (2 meningitis and 1 peridural intracranial abscess), while 2 patients had intratemporal complication (subperiostal abscess) associated to coalescent mastoiditis. We observed clinical profile of acute mastoiditis in regard to pathology found on the tympanic membrane, middle ear mucosa and destructions on the bony wall of the middle ear and mastoid. The main signs of progressive infection were tympanic membrane perforation, pulsatile suppurative secretion from the mucosa, and intratemporal abscess. All patients with coalescent mastoiditis required mastoidectomy, while noncoalescent mastoiditis was treated conservatively with broad-spectrum intravenous antibiotics and myringotomy. In conclusion acute mastoiditis is uncommon but serious complication of acute otitis media in children associated with significant morbidity. Coalescent mastoiditis concomitant with subperiostal abscess, intracranial complications and mastoiditis not responsive after 48 hours to intravenous antibiotics should urge clinician to timely mastoid surgery.
Intensive Observation of Toxic Side Effects After Several-Year of Cyclosporin Treatment in Kidney Transplant Patient
Authors: Ašćerić Mensura , Avdić Sevleta , Nukić Sabrija , Vrabac-Mujčinagić Muamera
Published: February 2018

In this work we are going to show results of intensive observation of adverse reactions of cyclosporine therapy during 18 months. The research was applied on 30 patients with kidney transplant. The medium time of kidney transplant survival was 9,7+/-2,3 years, with time span of 6 to 15 years. All the patients were subjects to several years' cyclosporine treatment, which was applied on a daily basis with a dosage of 2 to 5 mg/kg of body weight. The concentration of cyclosporine in blood was measured once a month. The concentration of cyclosporine in blood in 19 patients was in referent values of 122,50 nag/ml up to 280,50 nag/ml of blood. In 4 of the patients the concentration was heightened up to 370 to 538 nag/ml (X=766,37 nag/ml), and in 7 patients cyclosporine was below normal dosage down to 30,78 to 96,30 nag/ml in blood (x=77,12 nag/ml). We noticed these toxic side effects: increased values of systolic and diastolic arterial blood pressure in 5 patients, neurotoxic tremor effects in 4 patients, hyperplasia gingival and hirsute in 1 patient each.
Diverse Stature Estimation Formulae Applied to a Bosnian Population
Authors: Sarajlić Nermin , Cihlarž Zdenko
Published: February 2018

Since 1996, the Trotter formulae, developed on American Whites, have been used almost without exception to determine the stature of the population in Bosnia and Herzegovina. In 2002, Ross and Konigsberg presented new formulae for stature estimation for Balkans, using bones from unidentified Bosnian and Croatian males, victims of the recent war. The same year, 2002, in his master thesis, Sarajlić also presented formulae for stature estimation of Bosnian population. The research was undertaken on male cadavers. The cadaver length was measured directly and the length of the long bones was obtained from radiographs. However, none of these formulae were tested on the bones from exhumed persons. This study compares all three methods on a large independent sample in order to determine which formulae provide the most accurate stature estimation for the male population in Bosnia and Herzegovina. The sample for this research consists of long bones (humerus, femur, tibia and fibula) and was obtained from 369 exhumed and identified Bosnian males. Bones from the left side of the body were tested. Not all of them were present in each case: 211 left humeri, 270 left femura, 273 left tibiae and 175 left fibulae. Data about height was recollected by family members. Apart of whole sample, all three methods were applied separately on individuals taller then 180 cm. Of all three tested methods formulae proposed by Sarajlić et al. folowed by Ross and Konigsberg formulae, produced significantly better results in stature estimation of tall persons.
Epileptic Psychoses – Evaluation of Clinical Aspects
Authors: Fišeković Saida , Burnazović Lejla
Published: February 2018

Epileptic psychoses as the most complex psychopathological phenomena represent unexplored states for prognosis. Clinical trials, conveyed in order to found risk factors still are not consistent in their conclusions. By this research results of all biological, clinical, psychological and social, as well as demographic factors will contribute to opportunity to find variables which could finally prevent these conditions. In this study we tried to evaluate clinical variables which could foresee manifestation of interictal and postictal psychosis. This research study is epidemiological, clinical, retrospective and analytical. In total 567 patients were included in this study, which belonged to the diagnostic criteria F 0.6 according to ICD-10 classification, among which 14 patients with the diagnosed epileptic psychosis (06,8) were followed. All patients were hospitalized and evaluated at the Psychiatric Clinic in University of Sarajevo Clinics Centre, during time period between 01.01.2000 - 31.08.2006. Within baseline all relevant clinical and demographic variables were evaluated. Among patients most dominant form of behavior was expansive, with emphasized paranoid ideations and perception of auditory hallucinations. A correlation between intensive psychological trauma as an exacerbation factor and prolonged illness is determined, as well as between expression of psychotic symptoms and forced normalization by antiepileptic medications. Also is proven that among postictal psychosis more dominant are suicide attempts and aggressive behavior.
The Presence of Health-Risk Behaviour in Roma Family
Authors: Nikšić Dragana , Kurspahić - Mujčić Amira
Published: February 2018

Roma people in B&H are a marginalised population group. Their health condition; is considerably worse than the condition of other population groups. The health problems of Roma people correlate with inadequate living and dwelling conditions. Roma children are facing the impossibility of being health care beneficiaries, because their parents are unemployed. The objective of this survey was to examine the family surrounding of children in age up to 8 years, including the social conditions under which they live and the presence of health risk behaviour. The research presents a descriptive cross-section study. We interviewed 1100 non-Roma parents and children and 383 Roma parents and children (in the communities of domicile Roma people) in B&H Federation. The results obtained indicate that only 17,8 % of Roma parents are secondary-school leavers, while remaining percentage covers those with incomplete primary school or without education at all, against 63,6% of non-Roma parents who have secondary education. The parents consider themselves good providers for their children (59,3% of Roma parents and 75% of non-Roma parents often play with their children). The Roma parents seek for medical attention for their children only in the cases when urgent health problems occur, such as fever/increased body temperature (one-half of the interviewed parents) or diarrhoea (31,9%). Physical punishment of children occurs more frequently in Roma families (23,7% - this is only the top of an iceberg) then in non-Roma families (11,4%). The parents usually beat children by using their hands or punish them by flogging. The domestic violence is accepted amongst Roma people and it has most sever impact on children, who suffer emotionally and physically. In the future, it will be necessary to create the kind of family environment that would enable improvement of health condition and decrease the behaviour that endangers the health of children.
Pleural Drainage and its Role in Management of the Isolated Penetrating Chest Injuries During the War Time in Sarajevo, 1992.-1995.
Authors: Hadžismajlović Ademir , Pilav Alen
Published: February 2018

Penetrating chest injuries are the most frequent causes of serious demage and death in wounded indivisuals. In reports from the last wars where wounds caused by high velocity projectiles predominated, thoracotomies were perfomed in about 15% of the wounded individuals, mostly encompassing injuries of the heart and great vessels, accomanied by massive bleeding that could not be resolved by chest tube insertion. This retrospective analysis was performed on the medical records of 477 patients tretaed for isolated penetrating chest injuries in Department of Thoracic Surgery Clinical Center of the University in Sarajevo between april 1992 - june 1995. We analised the ways of their menagement with special view on pleural drainage, indication for this method and results of treatment. 398 (83,4%) wounded individuals have been treated with pleural tube inserting as definitive mesaure and for the urgent thoracotomy there were 79 (16,6%) patients left. Average hospital treatment in wounded drained patients was 7,68 days. With shrapnels there were 357 (74,84%) wounded individuals, and with bullet 120 (25,16%) wounded individuals. The complications of plaural tube inserting were - empyema in 34 (7,13%) patients and there were no other complications. Chest tube inserting as definitive mesaure was used in 398 (83,44%) patients. Chest tube inserting as preoperative measure (urgent thoracotomy) was used in 79 (16,56%) patients. There were 460 (96,44%) healed patients. Death occurred in 17 (3,56%) patients.
Registration and Analysis of Bioelectric Activity of Sensory-Motor Cortex During the Electrical Stimulation of Nucleus Caudate in Rats
Authors: Medenica-Milanović Snežana , Ristić Siniša , Turuntaš Vladimir , Mirić Mirjana , Kulić Milan
Published: February 2018

Background and purposeThe caudate circuit takes part in cognitive control of motor activity The purpose of the present work was registration and analysis of basic bioelectrical activity of ventral and dorsal sensory-motor cortex and nucleus caudate, study of the changes in EEG after nucleus caudate electrical stimulation and to identify of threshold level of electrical stimuli responsible for changes of electrical activity in registered brain area.Materials and methodsWe used 28 albino Wistar rat of both genders. After the animal fixation on stereotaxic apparatus to dry bone, the places for electrode fixation were marked. Two days after the electrodes had been implanted an EEG was registered so that the animals would adjust to the conditions and so they would repair the tissue reactions. EEG was registered with bipolar electrodes with ten-channeled apparatus. For first half an hour spontaneous activity of the brain was registered, and after that the head of nucleus caudate was stimulated with altered impulses of various voltages, frequency and duration.Results and conclusionsThreshold values of electric stimulus intensity from 3 to 5 V, frequency from 3 to 5 Hz, duration from 3 to 5 ms, by stimulation the head of nucleus caudate of rat, lead to the change of basal bioelectric activity of cerebrum. The change of bioelectric activity is firstly recorded in equilateral cortex, and with the higher intensity of the stimulus the changes overtake the contra lateral cortex.
Detection of the Hippocampal Formation Asymmetry in Patients With Posttraumatic Stress Disorder
Authors: Sarač-Hadžihalilović Aida , Dilberović Faruk
Published: February 2018

The goal of this work was to analyze asymmetry of hippocampal formation in PTSD patients. The objects of the analysis were 10 MRI scans of patients with PTSD with established cognitive function damage. MRI scans were obtained using MAGNET IMPACT SIEMENS 1,0 TESLA in T1 and T2 relaxation. The measurement of hippocampal formation dimensions, and comparisons between the right and the left side were done using evaluate - distance program on the MRI instrument of the Institute of Radiology at Sarajevo Clinics Center. We tested 20 patients with PTSD 49,9 years of average age with standard deviation of 4,62 years. Based on the analysis of our results regarding the size of hippocampal formation in all the three projections (axial, coronal and sagital), in the group of patients with PTSD, we can the make the following observations: In axial slice the length of hippocampal formation measured on the left and right side is significantly asymmetric in all patients. In the sagital slice, the hippocampal formation measured on the left side is in many cases longer than in the right - 50 %.In coronal slice no significant differences were found in the proportion of patients according to symmetry/asymmetry of hippocampal formation width on the left and right.The difference in average size of hippocampal formation between the left and right side measured in axial and coronal slices is not statistically significant, but it is significant in sagitall slice.So, the results of this new research, showed smaller hippocampal dimensions in PTSD as measured using MRI. We have to be careful about what projection we are refusing to as being watched hippocampal formation because the results will depend on that. We suggest that future studies be done in more projections because of the importance of statistically significant conclusions. MRI - volumetric measurements have their value.
The Prevalence of Cariogenic Salivary Micro-Organisms in Children of Various Ages
Authors: Nakaš Enita , Zukanović Amila
Published: February 2018

The objective of the research was to obtain data on the values of salivary Streptococcus mutans and Lactobacillus spp. in 12 years old children and teenagers (13 - 18 years old children), and to compare the values of these cariogenic microoorganisms in children of various ages, for the purpose of valuating the risk of caries. The sample comprised 32 persons divided into two groups. The first group included 17 children of the age of 12. The second group included 15 teenagers. The specimen of stimulated saliva, needed for the purpose of establishing the quantities of Streptococcus mutans and Lactobacillus spp., was taken by the method of CRT’-bacteria, according to manufacturers instruction.In the group of 12 years old children, the distribution of salivary Lactobacillus spp. was as follows: very low values 41,1%, low values 35,2%, high values 17,6%, very high values 5,8%, while in the group of teenagers, the results were the following: very low values 26,66%, low values 33,33%, high values 40%, very high values 0%. The levels of Streptococcus mutans were the following: 12 years old children had very low values in 41,7% cases, low values in 11,7% cases, high values in 41,7% cases, very high values in 5,8% cases, while the group of teenagers had very low values in 0%, low values in 33,3%, high values in 33,3%, and very high values in 33,3% cases.The levels of Streptococcus mutans are higher in our teenage children than in younger children, while the differences in levels of Lactobacillus spp. among children of various ages do not exist. In terms of global distribution, the levels of Streptococcus mutans and Lactobacillus spp. are higher in our population than in other countries in both control groups.
Clinical Outcome and Hormone Profiles Before and After Laparoscopic Electroincision of the Ovaries in Women With Polycystic Ovary Syndrome
Authors: Godinjak Zulfo , Javorić Ranka
Published: February 2018

The aim of study was to evaluate clinical outcome and hormone profiles of laparoscopic elec-troincision of the ovaries in women with polycystic ovary syndrome (PCOS) before and after treatment. Forty five clomiphene-citrate resistant women with polycystic ovary syndrome underwent laparoscopic electroincision of the ovaries. Serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T), androstenedione, 17 OH progesterone and beta endorphins were recorded before and 24 hours after the treatment. Clinical and reproductive outcome and hormone profiles were analyzed. Patients were observed during 12 months period. Laparoscopic electroincision of the ovaries was successfully performed without complications in all patients. LH/FSH ratio was 1,66 24 hours after treatment. Serum levels of T, androstenedione, 17 OH progesterone, and beta endorphins were significantly reduced 24 hours after laparoscopic electroincision of the ovaries. In follow-up period 87% of patients were recorded to have regular menstrual cycles and 61% pregnancy rate was achieved spontaneously. Laparoscopic electroincision of the ovaries is an effective treatment in clomiphene-citrate resistant women with polycystic ovary syndrome. The high pregnancy rate of the procedure offers a promising management for patients with polycystic ovary syndrome.
Testicular Metastasis of Gastrointestinal Stromal Tumor of the Jejunum
Authors: Dorić Mirsad , Radović Svjetlana , Babić Mirsad , Hukić Ajna , Kuskunović Suada , Tomić Ivana , Selak Ivan
Published: February 2018

Gastrointestinal stromal tumors (GIST) are neoplasm of mesenchymal origin that usually begins in cells of the wall of the gastrointestinal tract. It can be benign or malignant. In this report, we have presented a case of malignant GIST with uncommon site of metastasis. This is of interest because of three reasons. Firstly, metastases to the testis are extremely rare. However, metastases to distally localized organs are not commonly associated with GIST, and finally, to our knowledge this is the first case of malignant GIST metastasis to the testis reported in the world.
Physico-Chemical Characterisation Of Different Clindamycin Phosphate Samples
Authors: Vranić Edina , Planinšek Odon , Tivadar Andrijana , Hadžović Sabira , Srčič Stanko
Published: February 2018

For the majority of the pharmaceutical dosage forms, the substances that are used maintain solid state under the standard storage conditions, i.e. powders. The interactions of pharmaceutical powders (active ingredient(s) and excipients) with liquids and vapors (particularly aqueous solutions and their vapors) occur almost always during the production process. From the physical point of view, the interactions among individual components may differ from the expected because chemically identical substances obtained from different producers vary very much. These differences influence either the production process and/or the pharmaceutical form properties. In order to overcome these problems it is necessary to establish a control over the physico-chemical properties of the used materials.The aim of this work was to determine physico-chemical properties of three powder clindamycin phosphate samples (labeled as sample S1, S2 and S3) acquired through different suppliers. All the analysis were made for the purpose of establishing possible differences among the tested samples that showed variable physical stability in the solution: recrystallization of the S3 sample in the aqueous solution has been established during storage under standard conditions. On the basis of the obtained data it was possible to recognize the differences among the tested clindamycin phosphate samples and to explain the anomalous behavior of one sample.The surface free energy components for the investigated clindamycin phosphate samples were determined using Wu and Goodvan Oss method. The investigated clindamycin phosphate samples exhibit certain differences in surface free energy values as well as in surface morphology and thermal behavior. Comparison of γ+ and γ- values leads to the conclusion that all three clindamycin phosphate samples perform as monopolar, more electron acceptors, i.e. Lewis acids. However, an important difference exists between samples S1 and S2 on one and S3 on the other side. Sample S3 exhibits stronger acidic behavior, what could be connected with its recrystallization during the storage.The samples S1, S2 and S3 have different melting points e.g. “onset” temperatures. When the melting points move towards 200oC, the width of the “onset” temperature peak is especially important. In the case of wider peak, the potential for recrystallization seems to be higher.According to the stated, the sample S1 would be the “sample of choice” for the formulation of the stable pharmaceutical dosage form and has not shown any recrystallization tendencies during the storage period.
Tumor Suppresser Gene p53 Expression in Premalignant Lesions and Gastric Carcinoma - Prognostic Value
Authors: Vukobrat-Bijedić Zora , Radović Svjetlana , Husić-Selimović Azra , Gornjaković Srđan
Published: February 2018

The aim of the study was to verify the presence of mutated tumor suppresser gene p53 in intestinal mucosa with histologically confirmed premalignant lesions and gastric carcinoma, and assess its prognostic value. The paper presents prospective study that included 50 patients with gastric adeno-carcinoma of intestinal type that were treated at Gastroenterohepatology Clinic, and 50 patients with histologically confirmed chronic atrophic H. pylori positive gastritis. In the mucosa biopsy samples, we analyzed presence, frequency and severity of inflammatory-regenerative, metaplastic and dysplastic changes. We typed intestinal metaplasia immunohistochemically and confirmed the presence of p53 onco-protein in antigen positive gastric carcinoma cells, and evaluated its prognostic value. Our results suggest that H. pylori acts as an initiator of inflammatory processes in gastric mucosa, which are followed by emergence of precancerous lesions. p53 is expressed late in carcinogenesis (14%) and as such, may be considered as an indicator of transformation of premalignant into malignant lesion.
ß-endorphins as Possible Markers for Therapeutic Drug Monitoring
Authors: Jadrić Radivoj , Kiseljaković Emina , Hasić Sabaheta , Winterhalter-Jadrić Mira
Published: February 2018

This study was performed in order to investigate possible role of brain beta-endorphins as markers of antidepressive drugs therapy monitoring. Experiment was done using amitriptyline and trazodone as antidepressants. For quantification of brain beta-endorphins we used RIA technique. Our results showed significant decrease of brain beta-endorphins concentration in drug-pretreated animals, vs. those in of control group treated with 0,95% NaCl. The lower values were obtained in trazodone pre-treated animals. This study shows that use of psychoactive drugs have influence on brain beta-endorphins concentration. beta-endorphins could be of great importance, used as markers for evaluation of patient treatment.
Left Venticular Diastolic Dysfunction in Essential Hypertension
Authors: Avdić Sevleta , Mujčinović Zulfo , Ašćerić Mensura , Nukić Sabrija , Kušljugić Zumreta , Smajić Elnur , Arapčić Sedija
Published: February 2018

Diastolic dysfunction is very frequent and is actually sign of manifest heart weakness. Over 40% of patients with heart weakness have isolated left ventricular diastolic dysfunction (LVDD). New diagnostics methods as Doppler Echocardiography with close monitoring enables precise and early LVDD diagnose. In all diastolic phases artery hypertension weakens relaxation and left ventricular hypertrophy (LVH) weakens compliance also. The purpose of this study is to demonstrate importance of all LVDD. Doppler echocardiography parameters usage and its important echocardiography characteristic in case of hypertensive patients. This study represents 64 patients with essential hypertension - random sample. Three patients had atrial fibrillation. Besides anamnestic data collection, echocardiography evaluation was undertaken on all patients. For LVDD diagnose following parameters were used: isovolumic relaxation time (IVRT), peak early filling velocity (E), peak atrial filling velocity (A), E/A ratio, DT (deceleration time), left ventricular (LV) mass. Left ventricular hypertrophy (LVH) was verified for 57 patients. Seven hypertensive patients didn't have verified LVH. Comparing patients with LVH with those without LVH differences were observed: patients with LVH had a longer IVRT, lower E/A ratio, A wave growth, IVRT directly correlates with LV mass increase and backward correlation LV mass with E/A was noticed. Among patients with LVH with E/A ratio =or> 1-1,5 and based on transmitral flow we used IVRT duration and pulse Doppler with volume sample over lateral mitral annulus measuring mitral annulus velocity. It appeared that it corresponds with IVRT duration in LVDD evaluation. Patients with atrial fibrillation had considerably extended IVRT that indicates LVDD existence. Patients with left ventricular hypertrophy were older and they have higher left ventricular mass comparing with patients without left ventricular hypertrophy. In case of patients with essential hypertension all above mentioned LVDD parameters have to be defined, specially IVRT duration for determination of LVDD existence in case of all patients with essential hypertension with and without LVH and in case of associated atrial fibrillation presence. It is necessary to tend to, as early as possible, detect LVDD and it's prevention with improved essential hypertension monitoring.
Observing the Asymmetry of Amygdaloid Complex in Patients with Complex Partial Attacks
Authors: Sarač - Hadžihalilović Aida , Dilberović Faruk , Kučukalić Abdulah
Published: February 2018

Lobus limbicus is an anatomical basis for understanding the temporal epilepsy because it includes not only the focus of temporal lobe infection but of the frontal lobe as well. With it we can explain many of the phenomena accompanying epilepsy (hallucinations, the change of the effects, and so on). The goal of this assignment was to explore the asymmetry of amygdaloidal complex in the patients with complex partial attacks. The results show that the smallest number of patients with epilepsy have a symmetric (same size) amygdaloidal complex on the left side and the right. According to the asymmetry direction the difference in the number of patients with epilepsy is not statistically significant. Coefficient of asymmetry shows that the asymmetry on the left side is more frequent in men, while women have the same distribution on both sides. The greatest differences were found when considering the age factor. So, in all the three groups of evaluated data the differences in average age of patients with epilepsy according to total symm./asymm. were not statistically significant. But, the differences in average age depending on the direction of asymmetry were significant. Patients with longer amygdaloidal complex on the left side are significantly younger, both male and female (related to the axial slice, ant. - post. diameter). Thus, we propose the application of MRI technique in examining the asymmetry of the amygdaloidal complex that we used in this assignment as a template for future examinations in a sense of shedding light on the anatomical functions that underlie neuro-psychiatric dysfunctions.
Effects of Physical Therapy in the Treatment of the Posttraumatic Elbow Contractures in the Children
Authors: Jandrić Slavica
Published: February 2018

Pediatric post-traumatic elbow contracture can occur as result of the various injuries of area of the elbow joint. The aim of the study was to investigate the effect of the physical therapy and rehabilitation on the range of the motion of the elbow joint in the post-traumatic elbowcontractures in the childhood. We analyzed in this research 54 children (average age 9,4+/-3,15 years) that were treated because of posttraumatic elbow contractures. Kinesitherapy, occupational therapy and other procedures physical therapy are used in the management. Range of the motion of the elbow was measured at the beginning and the end of the therapy for every child. Functional outcome was presented in degree from 1 to 3 (Flynn). Satisfied result of the therapy at the discharge was in the 94, 45% of the children and 74, 07 % of the children had excellent result The difference in the grade at the beginning and the end of the therapy is statistically significant (t= 13,72, p<0,001). Significant improvement of the range of the motion in the elbow joint in the children with post-traumatic elbow contracture is attained by physical therapy.
Angiotensin Converting Enzyme Activity and Nitric Oxide Level in Serum of Patients with Dehydration
Authors: Huskić Jasminko , Čulo Filip , Dautović Sajma , Mulabegović Nedžad
Published: February 2018

Pediatric post-traumatic elbow contracture can occur as result of the various injuries of area of the elbow joint. The aim of the study was to investigate the effect of the physical therapy and rehabilitation on the range of the motion of the elbow joint in the post-traumatic elbowcontractures in the childhood. We analyzed in this research 54 children (average age 9,4+/-3,15 years) that were treated because of posttraumatic elbow contractures. Kinesitherapy, occupational therapy and other procedures physical therapy are used in the management. Range of the motion of the elbow was measured at the beginning and the end of the therapy for every child. Functional outcome was presented in degree from 1 to 3 (Flynn). Satisfied result of the therapy at the discharge was in the 94, 45% of the children and 74, 07 % of the children had excellent result The difference in the grade at the beginning and the end of the therapy is statistically significant (t= 13,72, p<0,001). Significant improvement of the range of the motion in the elbow joint in the children with post-traumatic elbow contracture is attained by physical therapy.
Surgical Treatment and Complications of Penile Fractures
Authors: Kulovac Benjamin , Aganović Damir , Junuzović Dželaludin , Prcić Alden , Hadžiosmanović Osman , Bazardžanović Mustafa , Hodžić Harun
Published: February 2018

Paper goal is to determine complications after urgent surgical treatment of patient with penile fractures, by using circumferential-degloving technique (degloving penile skin to root of penis). In period between 1998-2006 year, 23 patients have been treated as urgent cases with clinically proven penis fracture, age between 18 and 35. Patients were treated in 3 medical centers in Bosnia and Herzegovina(Sarajevo, Tuzla and Zenica). All 23 (100%) patients were injured during sexual intercourse. In the case of 20 (86,9%) patients partial rupture of corpus cavernosum was verified. Two (8,6%) patients had a complete rupture of urethra and it was primary sutured. In 22 cases (95,6%) spontaneous erection appeared, and in the case of 2 (8,6%) patients penile curvature was verified. Urgent surgical treatment is the best therapy choice, which enables preservation of erection in great number of cases in patients with penile fracture.
Acute Heart Failure After Myocardial Infarction
Authors: Macić-Džanković Amra , Pojskić Belma
Published: February 2018

We compared two groups of patients after acute myocardial infarction. First group was treated with fibrinolytics and they were hospitalized within six hours from the beginning of the first chest-symptoms, and second group that did not come within optimal time was treated with anticoagulants only. The patients were classified according to Killip-classification, shock-index and TIMI-risk-score after myocardial infarction. Results prove great benefit of fibrinolytic therapy in optimal time, concerning both keeping myocardial muscle mass and myocardial pump function.
A Comparation of Coronary Artery Bypass Grafting with and without Cardiopulmonary Bypass in Euroscore High Risk Patients
Authors: Kabil Emir , Mujanović Emir , Bergsland Jacob
Published: February 2018

Angiotensin converting enzyme (ACE) and nitric oxide (NO) have been suggested to be in - The objective of this study is to compare outcomes of coronary artery bypass grafting (CABG) in high-risk patients performed with- (ONCAB) and without -(OPCAB) use of cardiopulmonary bypass. From October 2001 till October 2005, 210 high-risk patients classified according to European System for Cardiac Operative Risk Evaluation (EuroSCORE) (score =or> 5) underwent CABG in Cardiovascular Clinic, University Clinical Centre Tuzla, Bosnia and Herzegovina. 138 patients operated as OPCAB were compared to 72 patients operated as ONCAB. All data were entered in a patient database (DATACOR) and analyzed in SPSS. OPCAB patients received insignificantly less number of grafts than those treated by ONCAB (3,0 vs. 3,2) (p=0,071). Stroke was significantly more common in ONCAB group (2,9 vs. 11,1%) (p=0,034) while the incidence of other postoperative complications and mortality were similar. The ventilation time (4,3 vs. 6,7 hours) (p=0,007), retransfusion volume (392,7 vs. 633,7 ml) (p=0,041) and hospital stay (8,2 vs. 10,1 days) (p=0,031) was significantly less in OPCAB group. OPCAB is safe and effective in treatment of high-risk patients. Avoidance of cardiopulmonary bypass is associated with reduced incidence of neurologic complications, lower intubation time, retransfusion rate and shorter hospital stay, and in our experience the preferred operative method in such patients.
Application of Euroscore in “Heart Center Sarajevo”
Authors: Mujičić Ermina , Ivanuša Mario , Omerbašić Edin , Štraus Slavenka , Perva Omer , Granov Nermir
Published: February 2018

Our aim was to evaluate risk stratification model, European System for Cardiac Risk Evaluation (logistic EuroSCORE) for patients treated in clinical hospital. EuroSCORE is useful to separate patients into risk groups so that the mortality and morbidity risk can be compared. From 1st January 2006 to 31st July 2006 the total of 124 adults have been operated and were classified according to the EuroSCORE algorithm. We have compared correlation of the predicted mortality and observed mortality (as death within the 30 days following the operation) and frequency of postoperative complications. All patients were divided into three risk groups. The low risk group had 30 patients with 0 death (0%) and 1 morbidity (3,33%). The medium risk group had 59 patients with 0 death (0%) and 4 morbidity (6,77%). The high risk group had 35 patients with 2 death (5,71%) and 5 morbidity (14,28%). Mortality in our clinic is much less than predicted mortality and we can be satisfied with our results. Incidence of complications after cardiac surgery is between 25 and 40% (STS database). Our results are within that range. We recommend logistic version of EuroSCORE as good and simple method to predict postoperative prognosis.
Inhibition of Neutral Red Photolysis with Different Antioxidants
Authors: Rimpapa Zlatan , Sofić Emin , Šapčanin Aida , Toromanović Jasmin , Tahirović Ismet
Published: February 2018

Neutral red is a dye the azine structure which has been used as an acido-base indicator and a dye in histochemistry. In 1960 Goldhaber introduced Neutral red into the medium of resorbing bone cultures to localize the osteoclast in the living cultures. Using time-lapse microcinematography in order to follow the osteoclasts, he reported excellent contrast could be obtained with Neutral red due to the avidity of osteoclasts for this dye. Unfortunately, however, the photodynamic effect resulting from subsequent exposure of these cultures to light precluded this approach, and again in 1963. it was observed that the death of the osteoclasts was probably due to a photodynamic effect related to the dye in the cell, the presence of oxygen and the frequent exposure of light by our time-lapse photography. VIS and UV irradiation induced photolysis of Neutral red, and from Neutral red cation produced with photons a Neutral red radical. This Neutral red radical can be inhibited with action of an antioxidant, such as melatonin, glutathione, ascorbic acid, E vitamin, etc. We developed an assay with Neutral redphotolysis which utilizes a VIS and UV irradiation technique for quantification the inhibition of photolysis with action of an antioxidant. In this method Neutral red acts double, as a free radical generator and as a photosensitizer.
Association of Hodgkin's lymphoma with Epstein Barr virus infection
Authors: Čičkušić Elmir , Mustedanagić-Mujanović Jasminka , Iljazović Ermina , Karasalihović Zinaida , Škaljić Ina
Published: February 2018

The role of Epstein Barr virus (EBV) in the onset of Hodgkin's lymphoma has been a subject of ongoing research. However, confirmation of EBV oncogenic involvement was not possible due to the small number of neoplastic cells characteristic for this type of tumor. Presence of EBV infection in neoplastic and non-neoplastic cells was analyzed in 81 cases of Hodgkin's lymphoma. In neoplastic cells, using an immunohistochemical method, latent membrane protein 1 (LMP1) was found in 33,3% of cases, while in situ hybridization results demonstrated the presence of EBER RNA in 48,1% of the cases. EBER RNA was found in non-neoplastic lymphocytes in 38,3% of cases. EBV is most frequently associated with Hodgkin's lymphoma in the first and seventh decade of life, specifically the nodular sclerosis subtype. No apparent difference was observed in the association of Hodgkin's lymphoma with EBV between genders, or in relation to clinical stage of the disease and average age of the patient. However, association with childhood age is significantly greater in comparison to adults. EBV associated disease shows a significantly greater prevalence in T lymphocytes. Slightly more abundant are cytotoxic T lymphocytes, which are also more frequently in contact with Reed-Sternberg cells, although there is no difference in number and positioning of histiocytes. Variations between the data on the association of EBV with Hodgkin's lymphoma among studies from different parts of the world suggest that factors of age, gender, ethnic background and social status might present biological modifiers of EBV influence on the pathogenesis of this neoplasm. The differences in non-neoplastic infiltrate EBV+ and EBV- lymphoma indicate the effect of the virus on the immune interaction of tumor and host in this disease.
Zearalenone-induced Lymphophagocytosis (T Cell Apoptosis) on the Rat’s Thymus
Authors: Dorić Mirsad , Radović Svjetlana , Babić Mirsad , Kuskunović Suada , Tomić Ivana , Selak Ivan
Published: February 2018

The effects of nonsteroidal mycotoxin zearalenone on the lymphoid tissue of thymus in a sense of investigating the subacute toxicity Wistar-albino rats have been examined in the course of the study. We analyzed 42 rats' specimens of both gender, treated with three dosage levels: 0,5; 2 and 4 mg/kg of body weight, after oral submission of the compound, and observed during three different time intervals: 10, 20 and 30 days. Microscopically was semiquantitatively determined lymphophagocytosis (apoptosis) and cortical thymic cellularity. It was percepted statistically significant growth of lymphophagocytosis compared to a dosage (p<0,01), as well as combination of dosage and interval (p<0,001), while gender had no statistically significant influence on tested parameter (p>0,05). Changes in cortical thyme cellularity were not percepted. Effects of applied doses of zearalenone on the lymphoid tissue of thymus were very mild and in correlation with estrogenicity. They are probably the result of interaction with estrogenic receptors.
Martin-gruber Anastomosis and Transposition in Cubital Tunnel
Authors: Isaković Eldar , Delić Jasmin , Bajtarević Alma
Published: February 2018

This describes the indivisible anastomosis of the main stem of median nerve with ulnar nerve through cubital tunnel followed by the anomaly of ulnar artery that appears as superficial ulnar artery. Both anomalies are found during the anatomical dissection of a grown-up male cadaver, on his right arm. Such case is very rare as, in the literature available to us, it has not been described.
Angiotensin Converting Enzyme Activity in Compensatory Renal Hypertrophy
Authors: Babić Nermina , Huskić Jasminko , Nakaš-Ićindić Emina
Published: February 2018

Serum and tissue (kidney's) angiotensin-converting enzyme (ACE) activity has been examined in Wistar rats (10 males and 10 females), seven days after unilateral nephrectomy. Renal hypertrophy was determined by measurement of kidney absolute mass. Serum and tissue ACE activity was determined by spectrophotometric method using hippuryl-l-histidyl-l-leucine (Hip-His-Leu) as a substrate. The ACE serum activity was expressed in units that correspond to 1 nmol of hippuric acid released by enzymatic hydrolysis of Hip-His-Leu substrate per minute/ml serum. The ACE tissue activity was expressed in units that correspond to 1 nmol of hippuric acid released by enzymatic hydrolysis of Hip-His-Leu substrate per minute/mg protein or mg kidney's tissue. The ACE serum activity significantly increased (p<0,05) seven days after unilateral nephrectomy. The ACE tissue activity, expressed in units that corresponds to 1 nmol of hippuric acid released by hydrolysis of Hip-His-Leu substrate per minute/mg protein, was higher seven days after unilateral nephrectomy then in kidney control, but the difference was not significant compared to the values determined in kidney control. The ACE tissue activity, expressed in units that correspond to 1 nmol of hippuric acid released by hydrolysis of Hip-His-Leu substrate per minute/mg tissue, was increased seven days after unilateral nephrectomy, which is statistically significant compared to the activity of the same enzyme in kidney control (p<0,01). The results indicate that ACE, probably has an important role in development of adaptive compensatory mechanisms after unilateral nephrectomy.