bluuebunny/crossref_metadata_2025 huggingface dataset:
Microbial Lipid Production from Corn Stover by the Oleaginous Yeast Rhodosporidium toruloides Using the PreSSLP Process
Authors: Dai Xiaozan , Shen Hongwei , Li Qiang , Rasool Kamal , Wang Qian , Yu Xue , Wang Lei , Bao Jie , Yu Dayu , Zhao Zongbao K.
Published: March 2019

Dry acid pretreatment and biodetoxification (DryPB) has been considered as an advanced technology to treat lignocellulosic materials for improved downstream bioconversion. In this study, the lipid production from DryPB corn stover was investigated by the oleaginous yeast Rhodosporidium toruloides using a new process designated prehydrolysis followed by simultaneous saccharification and lipid production (PreSSLP). The results found that prehydrolysis at 50 °C and then lipid production at 30 °C improved lipid yield by more than 17.0% compared with those without a prehydrolysis step. The highest lipid yield of 0.080 g/g DryPB corn stover was achieved at a solid loading of 12.5%. The fatty acid distribution of lipid products was similar to those of conventional vegetable oils that are used for biodiesel production. Our results suggested that the integration of DryPB process and PreSSLP process can be explored as an improved technology for microbial lipid production from lignocellulosic materials.
Numerical Simulation and Experimental Investigation of Diesel Fuel Reforming over a Pt/CeO2-Al2O3 Catalyst
Authors: Chen Hanyu , Wang Xi , Pan Zhixiang , Xu Hongming
Published: March 2019

In order to benefit from a realistic hydrogen production device equipped on a vehicle, issues with the effects of the process parameters on H2 and CO yield need to be resolved. In this study, a reduced mechanism for n-heptane (as a surrogate diesel) reforming over a Pt/CeO2-Al2O3 catalyst is adopted to investigate the effects of the process parameters on H2 and CO yield, and the preferred process parameters are concluded. In addition, the comparison of reforming bench tests of diesel fuel and n-heptane under typical diesel engine operating conditions is conducted. The n-heptane reforming simulation results show that the maximum H2 and CO yield moves toward unity with the decreased GHSV and increased reaction temperature, and the GHSV of 10,000 1/h, O2/C ratio of 0.6 and reaction temperature of 500 °C is preferable. The contrast experiments reveal that the change trend of H2 and CO yield displays consistence, although the difference of the average H2 and CO yield results is obvious. The characteristics of n-heptane reforming can represent H2 and CO yield features of diesel fuel reforming at typical reaction temperatures in a way.
Simultaneous Power Flow Decouple and Converter Gain Design for Electric Vehicle to Grid System
Authors: Cai Liangcheng
Published: March 2019

This paper presents a novel idea based on the Port-Hamiltonian and cascade system to control the electric vehicle-to-grid (V2G) system. Based on the proposed method, the relationship between the converter plant gain and the moment of inertia of generator that has been established is effective for decoupled control of the V2G system. It is well known that the existing methods apply the H ∞ control and PSO method to enumerate and choose the value of converter plant gain from the region decided by the state of charge. Since an explicit form of converter plant gain is obtain in this paper, the proposed method that is unnecessary to repeat the above existing details effectively reduces the calculated amount of converter plant gain. Finally, the simulations demonstrate the validity and advantage of the proposed method.
Acquisition of Forest Attributes for Decision Support at the Forest Enterprise Level Using Remote-Sensing Techniques—A Review
Authors: Surový Peter , Kuželka Karel
Published: March 2019

In recent decades, remote sensing techniques and the associated hardware and software have made substantial improvements. With satellite images that can obtain sub-meter spatial resolution, and new hardware, particularly unmanned aerial vehicles and systems, there are many emerging opportunities for improved data acquisition, including variable temporal and spectral resolutions. Combined with the evolution of techniques for aerial remote sensing, such as full wave laser scanners, hyperspectral scanners, and aerial radar sensors, the potential to incorporate this new data in forest management is enormous. Here we provide an overview of the current state-of-the-art remote sensing techniques for large forest areas thousands or tens of thousands of hectares. We examined modern remote sensing techniques used to obtain forest data that are directly applicable to decision making issues, and we provided a general overview of the types of data that can be obtained using remote sensing. The most easily accessible forest variable described in many works is stand or tree height, followed by other inventory variables like basal area, tree number, diameters, and volume, which are crucial in decision making process, especially for thinning and harvest planning, and timber transport optimization. Information about zonation and species composition are often described as more difficult to assess; however, this information usually is not required on annual basis. Counts of studies on forest health show an increasing trend in the last years, mostly in context of availability of new sensors as well as increased forest vulnerability caused by climate change; by virtue to modern sensors interesting methods were developed for detection of stressed or damaged trees. Unexpectedly few works focus on regeneration and seedlings evaluation; though regenerated stands should be regularly monitored in order to maintain forest cover sustainability.
Effects of Hygrothermal Environment in Cooling Towers on the Chemical Composition of Bamboo Grid Packing
Authors: Chen Li-Sheng , Fei Ben-Hua , Ma Xin-Xin , Lu Ji-Ping , Fang Chang-Hua
Published: March 2019

Bamboo grid packing (BGP) is a new kind of cooling packing, used in some Chinese hyperbolic cooling towers, which has excellent potential to complement or replace cooling packing made of polyvinyl chloride (PVC), cement, and glass fiber-reinforced plastic. For bamboo applications, mechanical properties and service life are matters of concern; this is strongly associated with bamboo’s chemical composition and mass loss. To better understand the mechanics of mechanical property deterioration and service life reduction, this study investigated the effects of hygrothermal environments in cooling towers on the chemical and elemental composition, mass loss, Fourier-transform infrared (FTIR) spectrum, and color changes of BGP. The results showed that BGP that had been in service for nine years in cooling towers exhibited major decreases in content of hemicellulose and benzene-ethanol extractives, as well as a significant increases in the content of α-cellulose and lignin. Exposure to the hygrothermal environment led to a decrease of oxygen content and around 8% mass loss, as well as an increase in carbon content compared to control samples. The hot water flow in cooling towers not only hydrolyzed hemicellulose, but also degraded some functional groups in cellulose and lignin. The lightness (L*) and chromaticity (a* and b*) parameters of the used BGP all decreased, except for the a* value of the outer skin. The total color change (ΔE*) of the inner skin of used BGP exceeded that of the outer skin.
Probabilistic Seismic Demand Analysis of a Bridge with Unbonded, Post-Tensioned, Concrete-Filled, Fiber-Reinforced Polymer Tube Columns
Authors: Rai Manisha , ElGawady Mohamed A. , Rodriguez-Marek Adrian
Published: March 2019

Ground motions at sites close to a fault are sometimes affected by forward directivity, where the rupture energy arrives at the site in a form of a very short duration pulse. These pulses impose a heavy demand on structures located in the vicinity of the fault. In this research, a probabilistic seismic demand analysis (PSDA) for a self-centering bridge is carried out. The bridge columns consisted of unbonded, post-tensioned, concrete-filled, fiber-reinforced polymer tubes. A bridge model was developed and non-linear time history analyses were performed. Three different methodologies that used spectral accelerations to predict structural responses were used, and a time-domain approach was used for PSDA. In addition to the three approaches, a time-domain PSDA methodology was also used. The results of the PSDA from the four approaches are compared, and the advantages of using the time-domain methodology are discussed. The results of the PSDA showed that for a site located very close to the fault (6 km in this study), earthquakes having a magnitude (Mw) as small as 6.5 can be significantly hazardous because the periods of pulses generated by small magnitude earthquakes coincide with the periods of the bridge. Since small magnitude events occur with greater frequency than large magnitude events, they can have important contributions to risk.
Development and Characterization of a Pilot-Scale Model Cocoa Fermentation System Suitable for Studying the Impact of Fermentation on Putative Bioactive Compounds and Bioactivity of Cocoa
Authors: Racine Kathryn C. , Lee Andrew H. , Wiersema Brian D. , Huang Haibo , Lambert Joshua D. , Stewart Amanda C. , Neilson Andrew P.
Published: March 2019

Cocoa is a concentrated source of dietary flavanols—putative bioactive compounds associated with health benefits. It is known that fermentation and roasting reduce levels of native flavonoids in cocoa, and it is generally thought that this loss translates to reduced bioactivity. However, the mechanisms of these losses are poorly understood, and little data exist to support this paradigm that flavonoid loss results in reduced health benefits. To further facilitate large-scale studies of the impact of fermentation on cocoa flavanols, a controlled laboratory fermentation model system was increased in scale to a large (pilot) scale system. Raw cocoa beans (15 kg) were fermented in 16 L of a simulated pulp media in duplicate for 168 h. The temperature of the fermentation was increased from 25–55 °C at a rate of 5 °C/24 h. As expected, total polyphenols and flavanol levels decreased as fermentation progressed (a loss of 18.3% total polyphenols and 14.4% loss of total flavanols during fermentation) but some increases were observed in the final timepoints (120–168 h). Fermentation substrates, metabolites and putative cocoa bioactive compounds were monitored and found to follow typical trends for on-farm cocoa heap fermentations. For example, sucrose levels in pulp declined from >40 mg/mL to undetectable at 96 h. This model system provides a controlled environment for further investigation into the potential for optimizing fermentation parameters to enhance the flavanol composition and the potential health benefits of the resultant cocoa beans.
Einstein-Gauss-Bonnet Gravity with Extra Dimensions
Authors: van de Bruck Carsten , Longden Chris
Published: March 2019

We consider a theory of modified gravity possessing d extra spatial dimensions with a maximally symmetric metric and a scale factor, whose ( 4 + d ) -dimensional gravitational action contains terms proportional to quadratic curvature scalars. Constructing the 4D effective field theory by dimensional reduction, we find that a special case of our action where the additional terms appear in the well-known Gauss-Bonnet combination is of special interest as it uniquely produces a Horndeski scalar-tensor theory in the 4D effective action. We further consider the possibility of achieving stabilised extra dimensions in this scenario, as a function of the number and curvature of extra dimensions, as well as the strength of the Gauss-Bonnet coupling. Further questions that remain to be answered such as the influence of matter-coupling are briefly discussed.
Monitoring and Multi-Messenger Astronomy with IceCube
Authors: Reimann René
Published: March 2019

IceCube currently is the largest neutrino observatory with an instrumented detection volume of 1 km3 buried in the ice-sheet close to the antarctic South Pole station. With a 4 π field of view and an up-time of >99%, it is continuously monitoring the full sky to detect astrophysical neutrinos. With the detection of an astrophysical neutrino flux in 2013, IceCube opened a new observation window to the non-thermal Universe. The IceCube collaboration has a large program to search for astrophysical neutrinos, including measurements of the energy spectrum of the diffuse astrophysical flux, auto- and cross-correlation studies with other multi-messenger particles, and a real-time alert and follow-up system. On 22 September 2017, the IceCube online system sent out an alert reporting a high-energy neutrino event. This alert triggered a series of multi-wavelength follow-up observations that revealed a spatially-coincident blazar TXS 0506+056, which was also in an active flaring state. This correlation was estimated at a 3 σ level. Further observations confirmed the flaring emission in the very-high-energy gamma-ray band. In addition, IceCube found an independent 3.5 σ excess of a time-variable neutrino flux in the direction of TXS 0506+056 two years prior to the alert by examining 9.5 years of archival neutrino data. These are the first multi-messenger observations of an extra-galactic astrophysical source including neutrinos since the observation of the supernova SN1987A. This review summarizes the different detection and analysis channels for astrophysical neutrinos in IceCube, focusing on the multi-messenger program of IceCube and its major scientific results.
Novel Insights into Plant Genome Evolution and Adaptation as Revealed through Transposable Elements and Non-Coding RNAs in Conifers
Authors: Liu Yang , El-Kassaby Yousry A.
Published: March 2019

Plant genomes are punctuated by repeated bouts of proliferation of transposable elements (TEs), and these mobile bursts are followed by silencing and decay of most of the newly inserted elements. As such, plant genomes reflect TE-related genome expansion and shrinkage. In general, these genome activities involve two mechanisms: small RNA-mediated epigenetic repression and long-term mutational decay and deletion, that is, genome-purging. Furthermore, the spatial relationships between TE insertions and genes are an important force in shaping gene regulatory networks, their downstream metabolic and physiological outputs, and thus their phenotypes. Such cascading regulations finally set up a fitness differential among individuals. This brief review demonstrates factual evidence that unifies most updated conceptual frameworks covering genome size, architecture, epigenetic reprogramming, and gene expression. It aims to give an overview of the impact that TEs may have on genome and adaptive evolution and to provide novel insights into addressing possible causes and consequences of intimidating genome sizes (20–30 Gb) in a taxonomic group, conifers.
Tubulin-Based DNA Barcode: Principle and Applications to Complex Food Matrices
Authors: Morello Laura , Braglia Luca , Gavazzi Floriana , Gianì Silvia , Breviario Diego
Published: March 2019

The DNA polymorphism diffusely present in the introns of the members of the Eukaryotic beta-tubulin gene families, can be conveniently used to establish a DNA barcoding method, named tubulin-based polymorphism (TBP), that can reliably assign specific genomic fingerprintings to any plant or/and animal species. Similarly, many plant varieties can also be barcoded by TBP. The method is based on a simple cell biology concept that finds a conveniently exploitable molecular basis. It does not depend on DNA sequencing as the most classically established DNA barcode strategies. Successful applications, diversified for the different target sequences or experimental purposes, have been reported in many different plant species and, of late, a new a version applicable to animal species, including fishes, has been developed. Also, the TBP method is currently used for the genetic authentication of plant material and derived food products. Due to the use of a couple of universal primer pairs, specific for plant and animal organisms, respectively, it is effective in metabarcoding a complex matrix allowing an easy and rapid recognition of the different species present in a mixture. A simple, dedicated database made up by the genomic profile of reference materials is also part of the analytical procedure. Here we will provide some example of the TBP application and will discuss its features and uses in comparison with the DNA sequencing-based methods.
Structure-Specific Endonucleases and the Resolution of Chromosome Underreplication
Authors: Falquet Benoît , Rass Ulrich
Published: March 2019

Complete genome duplication in every cell cycle is fundamental for genome stability and cell survival. However, chromosome replication is frequently challenged by obstacles that impede DNA replication fork (RF) progression, which subsequently causes replication stress (RS). Cells have evolved pathways of RF protection and restart that mitigate the consequences of RS and promote the completion of DNA synthesis prior to mitotic chromosome segregation. If there is entry into mitosis with underreplicated chromosomes, this results in sister-chromatid entanglements, chromosome breakage and rearrangements and aneuploidy in daughter cells. Here, we focus on the resolution of persistent replication intermediates by the structure-specific endonucleases (SSEs) MUS81, SLX1-SLX4 and GEN1. Their actions and a recently discovered pathway of mitotic DNA repair synthesis have emerged as important facilitators of replication completion and sister chromatid detachment in mitosis. As RS is induced by oncogene activation and is a common feature of cancer cells, any advances in our understanding of the molecular mechanisms related to chromosome underreplication have important biomedical implications.
Quantification of the Effect of Bridge Pier Encasement on Headwater Elevation Using HEC-RAS
Authors: Subedi Abhijit Sharma , Sharma Suresh , Islam Anwarul , Lamichhane Niraj
Published: March 2019

The deterioration of bridge substructure is a serious concern across the United States. The pier encasement is one of the most common practices for repairing and strengthening the bridge substructure. It is a rehabilitation process of existing pile piers during the repair or replacement of the bridge superstructure, which involves enclosing part of an existing pile pier with a polyethylene or PVC pipe large enough to provide at least three inches of concrete cover over the existing pier when filled. However, this process of enclosing pile piers might elevate water level due to increase in pier width, which could be hazardous in high-risk flood zones. Furthermore, it may create an adverse impact on the stability of the bridge due to scouring around the pier foundation. In order to gain knowledge on the backwater effect due to pile encasement, Hydraulic Engineering Center-River Analysis System (HEC-RAS) was used in this research to perform hydraulic simulations near the bridge sites. These simulations were carried out for various channel configurations and pier sizes with a wide range of flows, which resulted into 224 HEC-RAS models in order to investigate the effects of pile pier encasement on the headwater elevation. This study demonstrated that the water elevation measured in the upstream of the bridge showed no-rise condition, especially for wider channel sections with flatter slopes. However, the water elevation at the immediate upstream of the bridge was slightly higher, and the increasing pattern was only noticeable for a smaller channel width (20 ft), and specifically, for increased flow rate. As the area of flow was decreased resulting in increased water surface elevation due to encasement, a generic power equation in the form of Y = aXb was suggested for various channel slopes for the increased water surface elevation (Y) for each percentage decrease in channel area (X).
Optimization of Polyaluminum Chloride-Chitosan Flocculant for Treating Pig Biogas Slurry Using the Box–Behnken Response Surface Method
Authors: Li Yu , Li Leigang , Yasser Farouk Reham , Wang Yuanyuan
Published: March 2019

Flocculation can remove large amounts of nitrogen and phosphorus from wastewater, and the resulting nitrogen- and phosphorus-rich floc can be used to produce organic fertilizer. For biogas slurries containing high levels of nitrogen and phosphorus, ordinary flocculants can no longer meet the flocculation requirements. In this study, to fully utilize the advantages of the two flocculants and achieve efficient removal rates of nitrogen and phosphorus from a biogas slurry, chitosan (CTS) and polyaluminum chloride (PAC) were used as a composite flocculation agent to flocculate pig biogas slurries. The response surface method was used to study the effect of PAC added (PACadded) to the composite flocculant (CF), composite flocculant added (CFadded) to the biogas slurry and the pH on flocculation performance, and optimize these three parameters. In the tests, when the PACadded was 6.79 g·100 mL−1CF, the CFadded was 20.05 mL·L−1 biogas slurry and the pH was 7.50, the flocculation performance was the best, with an absorbance of 0.132 at a wavelength of 420 nm. The total phosphorus (TP) concentration was reduced from 214.10 mg·L−1 to 1.38 mg·L−1 for a removal rate of 99.4%. The total ammonia nitrogen (TAN) concentration was reduced from 1568.25 mg·L−1 to 150.27 mg·L−1 for a removal rate of 90.4%. The results showed that the CF could form larger flocs, and had greater adsorption capacity and more stable flocculation performance than ordinary flocculants. Furthermore, the CF could exhibit better chelation, electrical neutralization and bridge adsorption.
Environmental Justice in Industrially Contaminated Sites. A Review of Scientific Evidence in the WHO European Region
Authors: Pasetto Roberto , Mattioli Benedetta , Marsili Daniela
Published: March 2019

In the WHO European Region the topic of contaminated sites is considered a priority among environment and health themes. Communities living in or close to contaminated sites tend to be characterized by a high prevalence of ethnic minorities and by an unfavorable socioeconomic status so rising issues of environmental justice. A structured review was undertaken to describe the contents of original scientific studies analyzing distributive and procedural justice in industrially contaminated sites carried out in the WHO European Region in the period 2010–2017. A systematic search of the literature was performed. In total, 14 articles were identified. Wherever assessments on environmental inequalities were carried out, an overburden of socioeconomic deprivation or vulnerability, with very few exemptions, was observed. The combined effects of environmental and socioeconomic pressures on health were rarely addressed. Results show that the studies on environmental and health inequalities and mechanisms of their generation in areas affected by industrially contaminated sites in the WHO European Region are in their early stages, with exemption of UK. Future efforts should be directed to improve study strategies with national and local assessments in order to provide evidence for equity-oriented interventions to reduce environmental exposure and related health risks caused by industrial contamination.
Application of Ordinary Kriging and Regression Kriging Method for Soil Properties Mapping in Hilly Region of Central Vietnam
Authors: Gia Pham Tung , Kappas Martin , Van Huynh Chuong , Hoang Khanh Nguyen Linh
Published: March 2019

Soil property maps are essential resources for agricultural land use. However, soil properties mapping is costly and time-consuming, especially in the regions with complicated topographic conditions. This study was conducted in a hilly region of Central Vietnam with the following objectives: (i) to evaluate the best environmental variables to estimate soil organic carbon (SOC), total nitrogen (TN), and soil reaction (pH) with a regression kriging (RK) model, and (ii) to compare the accuracy of the ordinary kriging (OK) and RK methods. SOC, TN, and soil pH data were measured at 155 locations within the research area with a sampling grid of 2 km × 2 km for a soil layer from 0 to 30 cm depth. From these samples, 117 were used for interpolation, and the 38 randomly remaining samples were used for evaluating accuracy. The chosen environmental variables are land use type (LUT), topographic wetness index (TWI), and transformed soil adjusted vegetation index (TSAVI). The results indicate that the LUT variable is more effective than TWI and TSAVI for determining TN and pH when using the RK method, with a variance of 7.00% and 18.40%, respectively. In contrast, a combination of the LUT and TWI variables is the best for SOC mapping with the RK method, with a variance of 14.98%. The OK method seemed more accurate than the RK method for SOC mapping by 3.33% and for TN mapping by 10% but the RK method was found more precise than the OK method for soil pH mapping by 1.81%. Further selection of auxiliary variables and higher sampling density should be considered to improve the accuracy of the RK method.
Comparative Physiological Analysis Reveals the Role of NR-Derived Nitric Oxide in the Cold Tolerance of Forage Legumes
Authors: Zhang Peipei , Li Shuangshuang , Zhao Pengcheng , Guo Zhenfei , Lu Shaoyun
Published: March 2019

The role of nitric oxide (NO) signaling in the cold acclimation of forage legumes was investigated in this study. Medicago sativa subsp. falcata (L.) Arcang. (hereafter M. falcata) is a forage legume with a higher cold tolerance than Medicago truncatula, a model legume. Cold acclimation treatment resulted in increased cold tolerance in both M. falcata and M. truncatula, which was suppressed by pretreatment with tungstate, an inhibitor of nitrate reductase (NR), and 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (PTIO), a scavenger of NO. Likely, NITRATE REDUCTASE 1 (NIA1), but not NIA2 transcript, NR activity, and NO production were increased after cold treatment. Treatments with exogenous NO donors resulted in increased cold tolerance in both species. Superoxide dismutase (SOD), catalase (CAT), and ascorbate-peroxidase (APX) activities and Cu,Zn-SOD2, Cu,Zn-SOD3, cytosolic APX1 (cAPX1), cAPX3 and chloroplastic APX1 (cpAPX1) transcript levels were induced in both species after cold treatment, which was suppressed by tungstate and 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (PTIO). Treatment with exogenous NO resulted in enhanced activities of SOD, CAT, and APX. Moreover, higher levels of NIA1 transcript, NR activity, NO production, and antioxidant enzyme activities and transcripts were observed in M. falcata as compared with M. truncatula after cold treatment. The results suggest that NR-derived NO production and upregulated antioxidant defense are involved in cold acclimation in both species, while the higher levels of NO production and its derived antioxidant enzymes are associated with the higher cold tolerance in M. falcata as compared with M. truncatula.
Biological Activity and In Silico Study of 3-Modified Derivatives of Betulin and Betulinic Aldehyde
Authors: Bębenek Ewa , Chrobak Elwira , Marciniec Krzysztof , Kadela-Tomanek Monika , Trynda Justyna , Wietrzyk Joanna , Boryczka Stanisław
Published: March 2019

A series of 3-substituted derivatives of betulin and betulinic aldehyde were synthesized as promising anticancer agents. The newly triterpenes were tested against five human cancer cell lines like biphenotypic B myelomonocytic leukaemia (MV-4-11), adenocarcinoma (A549), prostate (Du-145), melanoma (Hs294T), breast adenocarcinoma (MCF-7) and normal human mammary gland (MCF-10A). The compound 9 showed towards Du-145, MCF-7 and Hs294T cells significant antiproliferative activity with IC50 ranging from 7.3 to 10.6 μM. The evaluation of ADME properties of all compounds also includes their pharmacokinetic profile. The calculated TPSA values for synthetized derivatives are in the range between 43.38 Å2 and 55.77 Å2 suggesting high oral bioavailability. The molecular docking calculations showed that triterpene 9 fits the active site of the serine/threonine protein kinase Akt.
UV-B Filter Octylmethoxycinnamate Induces Vasorelaxation by Ca2+ Channel Inhibition and Guanylyl Cyclase Activation in Human Umbilical Arteries
Authors: Lorigo Margarida , Quintaneiro Carla , Lemos Manuel C. , Martinez-de-Oliveira José , Breitenfeld Luiza , Cairrao Elisa
Published: March 2019

Ultraviolet (UV) filters are chemicals widely used in personal care products (PCPs). Due to their effect as endocrine disruptor compounds (EDCs), the toxicity of UV filters is a current concern for human health. EDC exposure may be correlated to cardiovascular diseases (CVD), but to our knowledge, no studies assessed the UV filters effects as human EDCs at the vascular level. Octylmethoxycinnamate (OMC) is the world’s most widely used UV-B filter, present in more than 90% of PCPs. Due to its demonstrated multiple hormonal activities in animal models, this substance is also suspected to be a human EDC. The purpose of this study was to assess the rapid/short-term effects of OMC on arterial tonus and analyse its mode of action (MOA). Using human umbilical arteries, the endocrine effects of OMC were evaluated in in vitro (cellular and organ) experiments by planar cell surface area (PCSA) and organ bath, respectively. Our data show that OMC induces a rapid/short-term smooth muscle relaxation acting through an endothelium-independent MOA, which seems to be shared with oestrogens, involving an activation of soluble guanylyl cyclase (sGC) that increases the cyclic guanosine monophosphate (cGMP) intracellular levels and an inhibition of L-type voltage-operated Ca2+ channels (L-Type VOCC).
Current Status and Future Prospects of Clinically Exploiting Cancer-specific Metabolism—Why Is Tumor Metabolism Not More Extensively Translated into Clinical Targets and Biomarkers?
Authors: Muthu Magesh , Nordström Anders
Published: March 2019

Tumor cells exhibit a specialized metabolism supporting their superior ability for rapid proliferation, migration, and apoptotic evasion. It is reasonable to assume that the specific metabolic needs of the tumor cells can offer an array of therapeutic windows as pharmacological disturbance may derail the biochemical mechanisms necessary for maintaining the tumor characteristics, while being less important for normally proliferating cells. In addition, the specialized metabolism may leave a unique metabolic signature which could be used clinically for diagnostic or prognostic purposes. Quantitative global metabolic profiling (metabolomics) has evolved over the last two decades. However, despite the technology’s present ability to measure 1000s of endogenous metabolites in various clinical or biological specimens, there are essentially no examples of metabolomics investigations being translated into actual utility in the cancer clinic. This review investigates the current efforts of using metabolomics as a tool for translation of tumor metabolism into the clinic and further seeks to outline paths for increasing the momentum of using tumor metabolism as a biomarker and drug target opportunity.
Efficacy of Valganciclovir Treatment Depends on the Severity of Hearing Dysfunction in Symptomatic Infants with Congenital Cytomegalovirus Infection
Authors: Ohyama Shohei , Morioka Ichiro , Fukushima Sachiyo , Yamana Keiji , Nishida Kosuke , Iwatani Sota , Fujioka Kazumichi , Matsumoto Hisayuki , Imanishi Takamitsu , Nakamachi Yuji , Deguchi Masashi , Tanimura Kenji , Iijima Kazumoto , Yamada Hideto
Published: March 2019

Although earlier studies have shown that antiviral treatment regimens using valganciclovir (VGCV) improved hearing function in some infants with congenital cytomegalovirus (CMV) infection; its efficacy on the severity of hearing dysfunction is unclear. We conducted a prospective study among 26 infants with congenital CMV infections from 2009 to 2018. Oral VGCV (32 mg/kg/day) was administered for 6 weeks (November 2009 to June 2015; n = 20) or 6 months (July 2015 to March 2018, n = 6). Hearing function was evaluated by measuring the auditory brainstem response before VGCV treatment and at 6 months. Hearing dysfunction, defined as a V-wave threshold >40 dB, was categorized into: most severe, ≥91 dB; severe, 61–90 dB; and moderate, 41–60 dB. Hearing improvement was defined as a decrease of ≥20 dB from the pretreatment V-wave threshold. Of 52 ears in 26 infants with congenital CMV infection, 29 (56%) had hearing dysfunction, and of 29 ears, 16 (55%) improved after VGCV treatment. Although, 16 (84%) of 19 ears with moderate or severe hearing dysfunction improved after treatment (p < 0.001), 10 ears with the most severe form did not. In conclusion, VGCV treatment is effective in improving moderate and severe hearing dysfunction in infants with congenital CMV infection.
High Incidence of Diabetes in People with Extremely High High-Density Lipoprotein Cholesterol: Results of the Kanagawa Investigation of Total Checkup Data from the National Database-1 (KITCHEN-1)
Authors: Nakajima Kei , Higuchi Ryoko , Iwane Taizo , Shibata Michi , Takada Kento , Sugiyama Michiko , Matsuda Masafumi , Nakamura Teiji
Published: March 2019

Background: It is unknown whether extremely high high-density lipoprotein cholesterol (HDL-C) has a protective effect against diabetes, which plays a key role in cardiovascular disease. Methods: In a community-based cohort study of 387,642 subjects (40–68 years old) without diabetes, the incidence of diabetes 6 years later was determined according to baseline HDL-C (≤39, 40–49, 50–59, 60–69, 70–79, 80–89, 90–99, 100–109, or ≥110 mg/dL). Results: At baseline, HDL-C ≥100 mg/dL was present in 12,908 subjects (3.3%), who had a better lipid profile and a high prevalence of heavy alcohol consumption and habitual exercise. The incidences of diabetes according to baseline HDL-C were 14.7, 11.2, 7.7, 5.3, 3.8, 2.8, 2.7, 2.5, and 3.5 per 1000 person-years, respectively. The adjusted relative risks (ARRs) for diabetes showed concave relationships with HDL-C, with minima at 80–89 mg/dL. The ARR (95% CI) of the lowest HDL-C category was 1.56 (1.40–1.74) and of the highest HDL-C category was 1.46 (1.18–1.81) (both p < 0.001), regardless of alcohol consumption. The latter ARR was higher in men (n = 219,047) (2.45 (1.70–3.53), p < 0.0001) after adjustment for baseline glycemic index. Conclusion: Both extremely high and low HDL-C represent risks for diabetes, which deserves further study.
Prediction of Glucose Intolerance in Early Postpartum in Women with Gestational Diabetes Mellitus Based on the 2013 WHO Criteria
Authors: Benhalima Katrien , Van Crombrugge Paul , Moyson Carolien , Verhaeghe Johan , Vandeginste Sofie , Verlaenen Hilde , Vercammen Chris , Maes Toon , Dufraimont Els , De Block Christophe , Jacquemyn Yves , Mekahli Farah , De Clippel Katrien , Van Den Bruel Annick , Loccufier Anne , Laenen Annouschka , Minschart Caro , Devlieger Roland , Mathieu Chantal
Published: March 2019

Predictors for glucose intolerance postpartum were evaluated in women with gestational diabetes mellitus (GDM) based on the 2013 World Health Organization (WHO) criteria. 1841 women were tested for GDM in a prospective cohort study. A postpartum 75g oral glucose tolerance test (OGTT) was performed in women with GDM at 14 ± 4.1 weeks. Of all 231 mothers with GDM, 83.1% (192) had a postpartum OGTT of which 18.2% (35) had glucose intolerance. Women with glucose intolerance were more often of Asian origin [15.1% vs. 3.7%, OR 4.64 (1.26–17.12)], had more often a recurrent history of GDM [41.7% vs. 26.7%, OR 3.68 (1.37–9.87)], higher fasting glycaemia (FPG) [5.1 (4.5–5.3) vs. 4.6 (4.3–5.1) mmol/L, OR 1.05 (1.01–1.09)], higher HbA1c [33 (31–36) vs. 32 (30–33) mmol/mol, OR 4.89 (1.61–14.82)], and higher triglycerides [2.2 (1.9–2.8) vs. 2.0 (1.6–2.5) mmol/L, OR 1.00 (1.00–1.01)]. Sensitivity of glucose challenge test (GCT) ≥7.2 mmol/l for glucose intolerance postpartum was 80% (63.1%–91.6%). The area under the curve to predict glucose intolerance was 0.76 (0.65–0.87) for FPG, 0.54 (0.43–0.65) for HbA1c and 0.75 (0.64–0.86) for both combined. In conclusion, nearly one-fifth of women with GDM have glucose intolerance postpartum. A GCT ≥7.2 mmol/L identifies a high risk population for glucose intolerance postpartum.
The Snell’s Window Image for Remote Sensing of the Upper Sea Layer: Results of Practical Application
Authors: Molkov Alexander A. , Dolin Lev S.
Published: March 2019

Estimation of water optical properties can be performed by photo or video registration of rough sea surface from underwater at an angle of total internal reflection in the away from the sun direction at several depths. In this case, the key characteristic of the obtained image will be the border of the Snell’s window, which is a randomly distorted image of the sky. Its distortion changes simultaneously under the action of the sea roughness and light scattering; however, after correct “decoding” of this image, their separate determination is possible. This paper presents the corresponding algorithms for achieving these possibilities by the Snell’s window images. These images were obtained in waters with different optical properties and wave conditions under several types of illumination. Practical guidelines for recording, processing and analyzing images of the Snell’s window are also formulated.
Monetary Policy, Cash Flow and Corporate Investment: Empirical Evidence from Vietnam
Authors: My Tran Linh , Mai Chi , Huu Le Phuoc , Bui Chi , Nguyen Linh , Huynh Toan
Published: March 2019

This paper examines the relationships between macroscopic determinants (typically, monetary policies) and microscopic factors (mainly, cash flows and other controlling variables) on corporate investment. By employing system-GMM estimation for the 250 Vietnamese non-financial firms, the authors find that the expansionary monetary policy not only encourages the borrowing activities but also results in more corporate investment activities over the period from 2006 to 2016. Noticeably, the internal cash flow is also significant factor, which enhances the activities of corporate investment. Finally, there are differences between internal cash flow effects on corporate investments between two groups, divided by three theoretical criteria. To recapitulate, our implications highlight the importance of monetary policy stability for sustainable growth in corporate investment in Vietnam.
Re-Evaluating the Climate Factor in Agricultural Land Assessment in a Changing Climate—Saskatchewan, Canada
Authors: Kerr Samantha A. , Andreichuk Yuliya , Sauchyn David J.
Published: March 2019

We established the statistical relationships between seasonal weather variables and average annual wheat yield (Hard Red Spring and Durum wheat: Triticum spp.) for the period of 1979–2016 for 296 rural municipalities (RMs) throughout six soil zones comprising the arable agricultural zone of Saskatchewan, Canada. Controlling climate variables were identified through Pearson’s product moment correlation analysis and used in stepwise regression to predict wheat yields in each RM. This analysis provided predictive regression equations and summary statistics at a fine spatial resolution, explaining up to 75% of the annual variance of wheat yield, in order to re-evaluate the climate factor rating in the arable land productivity model for the Saskatchewan Assessment and Management Agency (SAMA). Historical climate data (1885–2016) and Regional Climate Model (RCM) projections for the growing season (May–August) were also examined to put current climatic trends into longer-term perspective, as well as develop a better understanding of possible future climatic impacts on wheat yield in Saskatchewan. Historical trends demonstrate a decrease in maximum temperature and an increase in minimum temperature and precipitation throughout all soil zones. RCM projections also show a potential increase in temperatures and total precipitation by 5 °C and 10%, respectively. We recommended against a modification of the climate factor rating at this time because (1) any increase in wheat yield could not be attributed directly to the weather variables with the strongest trends, and (2) climate and wheat yield are changing more or less consistently across the zone of arable land, and one soil zone is not becoming more productive than another.
Land Use Evaluation over the Jema Watershed, in the Upper Blue Nile River Basin, Northwestern Highlands of Ethiopia
Authors: Taye Mintesinot , Simane Belay , F. Zaitchik Benjamin , G. Selassie Yihenew , Setegn Shimelis
Published: March 2019

Generating land capability class guidelines at a watershed scale has become a priority in sustainable agricultural land use. This study analyzed the area of cultivated land use situated on the non-arable land-capability class in the Jema watershed in the Upper Blue Nile River Basin. Soil surveys, meteorological ground observations, a digital elevation model (DEM) at 30 m, Meteosat at 10 km × 10 km and Landsat at 30 m were used to generate the sample soil texture class, average annual total rainfall (ATRF in mm), terrain, slope (%), elevation (m a.s.l) and land-use land cover (%). The land capability class was analyzed by considering raster layers of terrain, the average ATRF and soil texture. Geo-statistics was employed to fit a surface of soil texture and average ATRF estimates. An overlay technique was used to compute the proportion of cultivated land placed on non-arable land. As per the results of the terrain analysis, the elevation (m a.s.l) of the watershed is in the range of 1895 to 3518 m. The slope was found to be in the range of 0 to 45%. The amount of estimated rainfall ranged from 1640 to 131 mm with value declined from the lower to the higher elevation. Clay loam, clay and heavy clay were found to be the major soil texture classes. Four land capability classes, i.e., II, III, IV (arable) and V (non-arable), were identified with proportions of 28.56%, 45.74%, 22.16% and 3.54%, respectively. Seven land-use land covers were identified, i.e., annual crop land, grazing land, bush land, bare land, settlement land, forestland and water bodies, with proportions of 42.1, 35.9, 8.90, 8.3, 2.6, 2.1, and 0.2, respectively. Around 1707.7 ha of land in the watershed is categorized under non-arable land that cannot be used for annual crop cultivation at any level of intensity. Around 437 ha (3.5%) of land was cultivated on non-arable land. To conclude, the observed unsustainable crop land use could maximize soil loss in upstream regions and siltation and flooding downstream. The annual crop land use that was observed on non-arable land needs to be replaced with perennial crops, pasture and/or forest land uses.
Regenerative Endodontic Procedures Using Contemporary Endodontic Materials
Authors: Staffoli Simone , Plotino Gianluca , Nunez Torrijos Barbara G. , Grande Nicola M. , Bossù Maurizio , Gambarini Gianluca , Polimeni Antonella
Published: March 2019

Calcium hydroxide apexification and Mineral Trioxide Aggregate (MTA) apexification are classical treatments for necrotic immature permanent teeth. The first tend to fail for lack of compliance given the high number of sessions needed; the second has technical difficulties such as material manipulation and overfilling. With both techniques, the root development is interrupted leaving the tooth with a fragile root structure, a poor crown-to-root ratio, periodontal breakdown, and high risk of fracture, compromising long-term prognosis of the tooth. New scientific literature has described a procedure that allows complete root development of these specific teeth. This regenerative endodontic procedure (REP) proposes the use of a combination of antimicrobials and irrigants, no canal walls instrumentation, induced apical bleeding to form a blood clot and a tight seal into the root canal to promote healing. MTA is the most used material to perform this seal, but updated guidelines advise the use of other bioactive endodontic cements that incorporate calcium and silicate in their compositions. They share most of their characteristics with MTA but claim to have fewer drawbacks with regards to manipulation and aesthetics. The purpose of the present article is to review pertinent literature and to describe the clinical procedures protocol with its variations, and their clinical application.
Evaluation and Selection of De-Icing Salt Based on Multi-Factor
Authors: Ke Guoju , Zhang Jun , Tian Bo
Published: March 2019

De-icing salts can greatly ease traffic congestion but introduce corrosion of concrete and damage to plant growth. The decision of which de-icing salt to use becomes a crucial issue. In this study, several representative de-icing salts were investigated, and the effects of de-icing ability, salt freezing corrosion on concrete, and plant growth were comprehensively tested. Finally, the decision of de-icing salt was made based on analytic hierarchy process (AHP). Results show that de-icing salts achieving the best de-icing effect are not the same at different concentrations. De-icing salts of 3% concentration have the greatest corrosion to concrete. Notably, magnesium chloride and calcium magnesium acetate have the least impact on plants among all studied de-icing salts. Using AHP, ethylene glycol and calcium magnesium acetate are selected as optimal items under different priorities.
Advantages of the Application of the Temper Bead Welding Technique During Wet Welding
Authors: Tomków Jacek , Rogalski Grzegorz , Fydrych Dariusz , Łabanowski Jerzy
Published: March 2019

Thermo-mechanically rolled S460ML steel was chosen for welding in underwater wet welding conditions by covered electrodes. The main aim of this study was to check the weldability for fillet welds in a water environment by controlled thermal severity (CTS) tests and to check the influence of temper bead welding (TBW) on the weldability of the investigated steel. Non-destructive and destructive tests showed that S460ML steel has a high susceptibility to cold cracking. In all joints, hardness in the heat-affected zone (HAZ) was extended to the 400 HV10 values. Microscopic testing showed the presence of microcracks in the HAZ of all welded joints. TBW was chosen as the method to improve the weldability of the investigated steel. This technique allows for the reduction of the maximum hardness in the HAZ below the critical value of 380 HV10, as stated by the EN-ISO 15614-1:2017. It was determined that for S460ML steel, from the point of view of weldability, the pitch between two beads should be in the range 75%–100%. Also, if the pitch between two beads increases, the hardness, grain size, and number of cracks decreases. In all specimens where the hardness of the HAZ was below 380 HV10, there were no microcracks.
Mechanical and Cytocompatibility Evaluation of UHMWPE/PCL/Bioglass® Fibrous Composite for Acetabular Labrum Implant
Authors: Anindyajati Adhi , Boughton Philip , Ruys Andrew J.
Published: March 2019

In this study, a fibrous composite was developed as synthetic graft for labral reconstruction treatment, comprised of ultra-high molecular weight polyethylene (UHMWPE) fabric, ultrafine fibre of polycaprolactone (PCL), and 45S5 Bioglass®. This experiment aimed to examine the mechanical performance and cytocompatibility of the composite. Electrospinning and a slurry dipping technique were applied for composite fabrication. To assess the mechanical performance of UHMWPE, tensile cyclic loading test was carried out. Meanwhile, cytocompatibility of the composite on fibroblastic cells was examined through a viability assay, as well as SEM images to observe cell attachment and proliferation. The mechanical test showed that the UHMWPE fabric had a mean displacement of 1.038 mm after 600 cycles, approximately 4.5 times greater resistance compared to that of natural labrum, based on data obtained from literature. A viability assay demonstrated the predominant occupation of live cells on the material surface, suggesting that the composite was able to provide a viable environment for cell growth. Meanwhile, SEM images exhibited cell adhesion and the formation of cell colonies on the material surface. These results indicated that the UHMWPE/PCL/Bioglass® composite could be a promising material for labrum implants.