bluuebunny/crossref_metadata_2025 huggingface dataset:
Defining Food Safety Inspection
Authors: Barnes Jason , Whiley Harriet , Ross Kirstin , Smith James
Published: January 2022

Food safety inspections are a key health protection measure applied by governments to prevent foodborne illness, yet they remain the subject of sustained criticism. These criticisms include inconsistency and inadequacy of methods applied to inspection, and ineffectiveness in preventing foodborne illness. Investigating the validity of these criticisms represent important areas for further research. However, a defined construct around the meanings society attributes to food safety inspection must first be established. Through critical examination of available literature, this review identified meanings attributed to food safety inspection and explicates some of the key elements that compose food safety inspection as a social construct. A total of 18 meanings were found to be attributed to food safety inspection. Variation in meanings were found between consumers, food business associates and food safety inspectors. For some, inspection meant a source of assurance, for others a threat to fairness, while most view inspection as a product of resources and inspector training. The meanings were then examined in light of common criticisms directed at food safety inspection, to expound their influence in how food safety inspection is realized, shaped, and rationalized. This review highlights the influence of sociological factors in defining food safety inspection.
Neutrophil Cathepsin G Enhances Thrombogenicity of Mildly Injured Arteries via ADP-Mediated Platelet Sensitization
Authors: Nemmar Abderrahim , Hoylaerts Marc F.
Published: January 2022

Inhalation of particulate matter in polluted air causes direct, size-restricted passage in the circulation and pronounced lung inflammation, provoking platelet activation and (non)-fatal cardiovascular complications. To determine potency and mechanism of platelet sensitization via neutrophil enzymes, we performed in vitro aggregation studies in washed human platelets and in murine and human blood, in the presence of elastase, cathepsin G and regular platelet agonists, present in damaged arteries. The impact of both enzymes on in vivo thrombogenicity was studied in the same thrombosis mouse model, previously having demonstrated that neutrophil activation enhances peripheral thrombogenicity. At 0.05 U/mL, cathepsin G activated washed human platelets via PAR1, whereas at 0.35 U/mL, aggregation occurred via PAR4. In Swiss mouse platelet-rich plasma no aggregation occurred by cathepsin G at 0.4 U/mL. In human and murine blood, aggregations by 0.05–0.1 U/mL cathepsin G were similar and not PAR-mediated, but platelet aggregation was inhibited by ADP antagonists, advocating cathepsin G-released ADP in blood as the true agonist of sustained platelet activation. In the mouse thrombosis model, cathepsin G and elastase amplified mild thrombogenicity at blood concentrations that activated platelets in vitro. This study shows that cathepsin G and elastase secreted in the circulation during mild air pollution-induced lung inflammation lyse red blood cell membrane proteins, leading to ADP-leakage into plasma, sensitizing platelets and amplifying their contribution to cardiovascular complications of ambient particle inhalation.
The Regenerative Potential of Substance P
Authors: Redkiewicz Patrycja
Published: January 2022

Wound healing is a highly coordinated process which leads to the repair and regeneration of damaged tissue. Still, numerous diseases such as diabetes, venous insufficiencies or autoimmune diseases could disturb proper wound healing and lead to chronic and non-healing wounds, which are still a great challenge for medicine. For many years, research has been carried out on finding new therapeutics which improve the healing of chronic wounds. One of the most extensively studied active substances that has been widely tested in the treatment of different types of wounds was Substance P (SP). SP is one of the main neuropeptides released by nervous fibers in responses to injury. This review provides a thorough overview of the application of SP in different types of wound models and assesses its efficacy in wound healing.
Midazolam Ameliorates Hyperglycemia-Induced Glomerular Endothelial Dysfunction by Inhibiting Transglutaminase 2 in Diabetes
Authors: Seo Jae-Ah , Sayyed Nilofar Danishmalik , Lee Yeon-Ju , Jeon Hye-Yoon , Kim Eun-Bin , Hong Seok-Ho , Cho Soyeon , Kim Minsoo , Ha Kwon-Soo
Published: January 2022

Midazolam is an anesthetic widely used for anxiolysis and sedation; however, to date, a possible role for midazolam in diabetic kidney disease remains unknown. Here, we investigated the effect of midazolam on hyperglycemia-induced glomerular endothelial dysfunction and elucidated its mechanism of action in kidneys of diabetic mice and human glomerular microvascular endothelial cells (HGECs). We found that, in diabetic mice, subcutaneous midazolam treatment for 6 weeks attenuated hyperglycemia-induced elevation in urine albumin/creatinine ratios. It also ameliorated hyperglycemia-induced adherens junction disruption and subsequent microvascular leakage in glomeruli of diabetic mice. In HGECs, midazolam suppressed high glucose-induced vascular endothelial-cadherin disruption and endothelial cell permeability via inhibition of intracellular Ca2+ elevation and subsequent generation of reactive oxygen species (ROS) and transglutaminase 2 (TGase2) activation. Notably, midazolam also suppressed hyperglycemia-induced ROS generation and TGase2 activation in glomeruli of diabetic mice and markedly improved pathological alterations in glomerular ultrastructure in these animals. Analysis of kidneys from diabetic Tgm2−/− mice further revealed that TGase2 played a critical role in microvascular leakage. Overall, our findings indicate that midazolam ameliorates hyperglycemia-induced glomerular endothelial dysfunction by inhibiting ROS-mediated activation of TGase2.
Sporulation Strategies and Potential Role of the Exosporium in Survival and Persistence of Clostridium botulinum
Authors: Portinha Inês M. , Douillard François P. , Korkeala Hannu , Lindström Miia
Published: January 2022

Clostridium botulinum produces the botulinum neurotoxin that causes botulism, a rare but potentially lethal paralysis. Endospores play an important role in the survival, transmission, and pathogenesis of C. botulinum. C. botulinum strains are very diverse, both genetically and ecologically. Group I strains are terrestrial, mesophilic, and produce highly heat-resistant spores, while Group II strains can be terrestrial (type B) or aquatic (type E) and are generally psychrotrophic and produce spores of moderate heat resistance. Group III strains are either terrestrial or aquatic, mesophilic or slightly thermophilic, and the heat resistance properties of their spores are poorly characterized. Here, we analyzed the sporulation dynamics in population, spore morphology, and other spore properties of 10 C. botulinum strains belonging to Groups I–III. We propose two distinct sporulation strategies used by C. botulinum Groups I–III strains, report their spore properties, and suggest a putative role for the exosporium in conferring high heat resistance. Strains within each physiological group produced spores with similar characteristics, likely reflecting adaptation to respective environmental habitats. Our work provides new information on the spores and on the population and single-cell level strategies in the sporulation of C. botulinum.
The Role of Ghrelin/GHS-R1A Signaling in Nonalcohol Drug Addictions
Authors: Sustkova-Fiserova Magdalena , Charalambous Chrysostomos , Khryakova Anna , Certilina Alina , Lapka Marek , Šlamberová Romana
Published: January 2022

Drug addiction causes constant serious health, social, and economic burden within the human society. The current drug dependence pharmacotherapies, particularly relapse prevention, remain limited, unsatisfactory, unreliable for opioids and tobacco, and even symptomatic for stimulants and cannabinoids, thus, new more effective treatment strategies are researched. The antagonism of the growth hormone secretagogue receptor type A (GHS-R1A) has been recently proposed as a novel alcohol addiction treatment strategy, and it has been intensively studied in experimental models of other addictive drugs, such as nicotine, stimulants, opioids and cannabinoids. The role of ghrelin signaling in these drugs effects has also been investigated. The present review aims to provide a comprehensive overview of preclinical and clinical studies focused on ghrelin’s/GHS-R1A possible involvement in these nonalcohol addictive drugs reinforcing effects and addiction. Although the investigation is still in its early stage, majority of the existing reviewed experimental results from rodents with the addition of few human studies, that searched correlations between the genetic variations of the ghrelin signaling or the ghrelin blood content with the addictive drugs effects, have indicated the importance of the ghrelin’s/GHS-R1As involvement in the nonalcohol abused drugs pro-addictive effects. Further research is necessary to elucidate the exact involved mechanisms and to verify the future potential utilization and safety of the GHS-R1A antagonism use for these drug addiction therapies, particularly for reducing the risk of relapse.
Searching for New Z-DNA/Z-RNA Binding Proteins Based on Structural Similarity to Experimentally Validated Zα Domain
Authors: Bartas Martin , Slychko Kristyna , Brázda Václav , Červeň Jiří , Beaudoin Christopher A. , Blundell Tom L. , Pečinka Petr
Published: January 2022

Z-DNA and Z-RNA are functionally important left-handed structures of nucleic acids, which play a significant role in several molecular and biological processes including DNA replication, gene expression regulation and viral nucleic acid sensing. Most proteins that have been proven to interact with Z-DNA/Z-RNA contain the so-called Zα domain, which is structurally well conserved. To date, only eight proteins with Zα domain have been described within a few organisms (including human, mouse, Danio rerio, Trypanosoma brucei and some viruses). Therefore, this paper aimed to search for new Z-DNA/Z-RNA binding proteins in the complete PDB structures database and from the AlphaFold2 protein models. A structure-based similarity search found 14 proteins with highly similar Zα domain structure in experimentally-defined proteins and 185 proteins with a putative Zα domain using the AlphaFold2 models. Structure-based alignment and molecular docking confirmed high functional conservation of amino acids involved in Z-DNA/Z-RNA, suggesting that Z-DNA/Z-RNA recognition may play an important role in a variety of cellular processes.
Synergetic Fermentation of Glucose and Glycerol for High-Yield N-Acetylglucosamine Production in Escherichia coli
Authors: Wang Kaikai , Wang Xiaolu , Luo Huiying , Wang Yaru , Wang Yuan , Tu Tao , Qin Xing , Bai Yingguo , Huang Huoqing , Yao Bin , Su Xiaoyun , Zhang Jie
Published: January 2022

N-acetylglucosamine (GlcNAc) is an amino sugar that has been widely used in the nutraceutical and pharmaceutical industries. Recently, microbial production of GlcNAc has been developed. One major challenge for efficient biosynthesis of GlcNAc is to achieve appropriate carbon flux distribution between growth and production. Here, a synergistic substrate co-utilization strategy was used to address this challenge. Specifically, glycerol was utilized to support cell growth and generate glutamine and acetyl-CoA, which are amino and acetyl donors, respectively, for GlcNAc biosynthesis, while glucose was retained for GlcNAc production. Thanks to deletion of the 6-phosphofructokinase (PfkA and PfkB) and glucose-6-phosphate dehydrogenase (ZWF) genes, the main glucose catabolism pathways of Escherichia coli were blocked. The resultant mutant showed a severe defect in glucose consumption. Then, the GlcNAc production module containing glucosamine-6-phosphate synthase (GlmS*), glucosamine-6-phosphate N-acetyltransferase (GNA1*) and GlcNAc-6-phosphate phosphatase (YqaB) expression cassettes was introduced into the mutant, to drive the carbon flux from glucose to GlcNAc. Furthermore, co-utilization of glucose and glycerol was achieved by overexpression of glycerol kinase (GlpK) gene. Using the optimized fermentation medium, the final strain produced GlcNAc with a high stoichiometric yield of 0.64 mol/mol glucose. This study offers a promising strategy to address the challenge of distributing carbon flux in GlcNAc production.
Crosstalk between Oxidative Stress and Inflammatory Liver Injury in the Pathogenesis of Alcoholic Liver Disease
Authors: Yang Yoon Mee , Cho Ye Eun , Hwang Seonghwan
Published: January 2022

Alcoholic liver disease (ALD) is characterized by the injury, inflammation, and scarring in the liver owing to excessive alcohol consumption. Currently, ALD is a leading cause for liver transplantation. Therefore, extensive studies (in vitro, in experimental ALD models and in humans) are needed to elucidate pathological features and pathogenic mechanisms underlying ALD. Notably, oxidative changes in the liver have been recognized as a signature trait of ALD. Progression of ALD is linked to the generation of highly reactive free radicals by reactions involving ethanol and its metabolites. Furthermore, hepatic oxidative stress promotes tissue injury and, in turn, stimulates inflammatory responses in the liver, forming a pathological loop that promotes the progression of ALD. Accordingly, accumulating further knowledge on the relationship between oxidative stress and inflammation may help establish a viable therapeutic approach for treating ALD.
Climate Change Increases the Expansion Risk of Helicoverpa zea in China According to Potential Geographical Distribution Estimation
Authors: Zhao Haoxiang , Xian Xiaoqing , Zhao Zihua , Zhang Guifen , Liu Wanxue , Wan Fanghao
Published: January 2022

Helicoverpa zea, a well-documented and endemic pest throughout most of the Americas, affecting more than 100 species of host plants. It is a quarantine pest according to the Asia and Pacific Plant Protection Commission (APPPC) and the catalog of quarantine pests for plants imported to the People’s Republic of China. Based on 1781 global distribution records of H. zea and eight bioclimatic variables, the potential geographical distributions (PGDs) of H. zea were predicted by using a calibrated MaxEnt model. The contribution rate of bioclimatic variables and the jackknife method were integrated to assess the significant variables governing the PGDs. The response curves of bioclimatic variables were quantitatively determined to predict the PGDs of H. zea under climate change. The results showed that: (1) four out of the eight variables contributed the most to the model performance, namely, mean diurnal range (bio2), precipitation seasonality (bio15), precipitation of the driest quarter (bio17) and precipitation of the warmest quarter (bio18); (2) PGDs of H. zea under the current climate covered 418.15 × 104 km2, and were large in China; and (3) future climate change will facilitate the expansion of PGDs for H. zea under shared socioeconomic pathways (SSP) 1-2.6, SSP2-4.5, and SSP5-8.5 in both the 2030s and 2050s. The conversion of unsuitable to low suitability habitat and moderately to high suitability habitat increased by 8.43% and 2.35%, respectively. From the present day to the 2030s, under SSP1-2.6, SSP2-4.5 and SSP5-8.5, the centroid of the suitable habitats of H. zea showed a general tendency to move eastward; from 2030s to the 2050s, under SSP1-2.6 and SSP5-8.5, it moved southward, and it moved slightly northward under SSP2-4.5. According to bioclimatic conditions, H. zea has a high capacity for colonization by introduced individuals in China. Customs ports should pay attention to host plants and containers of H. zea and should exchange information to strengthen plant quarantine and pest monitoring, thus enhancing target management.
DSP Processer-in-the-Loop Tests Based on Automatic Code Generation
Authors: Zhang Qi , Pei Wenhui
Published: January 2022

The digital signal processing (DSP) processor-in-the-loop tests based on automatic code generation technology are studied. Firstly, the idea of model-based design is introduced, and the principle and method of embedded code automatic generation technology are analyzed by taking the automatic code generation of the DSP control algorithm for pulse width modulation (PWM) output as an example. Then, the control system model is established on MATLAB/Simulink. After verifying the model through simulation, the target board platform is established with DSP as the core processor, and the automatically generated code is tested by the processor-in-the-loop (PIL). The results show that the technology greatly shortens the development cycle of the project, improves the robustness and consistency of the control code, and can be widely used in the complex algorithm development process of the controller, from intelligent design and modeling to implementation.
Clinical Determinants and Prognosis of Left Ventricular Reverse Remodelling in Non-Ischemic Dilated Cardiomyopathy
Authors: Díez-López Carles , Salazar-Mendiguchía Joel , García-Romero Elena , Fuentes Lara , Lupón Josep , Bayés-Genis Antoni , Manito Nicolás , de Antonio Marta , Moliner Pedro , Zamora Elisabet , Catalá-Ruiz Pablo , Caínzos-Achirica Miguel , Comín-Colet Josep , González-Costello José
Published: January 2022

Aims: Non-ischaemic dilated cardiomyopathy (NIDCM) is characterized by left ventricular (LV) chamber enlargement and systolic dysfunction in the absence of coronary artery disease. Left ventricular reverse remodelling (LVRR) is the ability of a dilated ventricle to restore its normal size, shape and function. We sought to determine the frequency, clinical predictors and prognostic implications of LVRR, in a cohort of heart failure (HF) patients with NIDCM. Methods: We conducted a multicentre observational, retrospective cohort study of patients with NIDCM, with prospective serial echocardiography evaluations. LVRR was defined as an increase of ≥15% in left ventricular ejection fraction (LVEF) or as a LVEF increase ≥ 10% plus reduction of LV end-systolic diameter index ≥ 20%. We used multivariable logistic regression analyses to identify the baseline clinical predictors of LVRR and evaluate the prognostic impact of LVRR. Results: LVRR was achieved in 42.5% of 527 patients with NIDCM during the first year of follow-up (median LVEF 49%, median change +22%), Alcoholic aetiology, HF duration, baseline LVEF and the absence of LBBB (plus NT-proBNP levels when in the model), were the strongest predictors of LVRR. During a median follow-up of 47 months, 134 patients died (25.4%) and 7 patients (1.3%) received a heart transplant. Patients with LVRR presented better outcomes, regardless of other clinical conditions. Conclusions: In patients with NIDCM, LVRR was frequent and was associated with improved prognosis. Major clinical predictors of LVRR were alcoholic cardiomyopathy, absence of LBBB, shorter HF duration, and lower baseline LVEF and NT-proBNP levels. Our study advocates for clinical phenotyping of non-ischaemic dilated cardiomyopathy and intense gold-standard treatment optimization of patients according to current guidelines and recommendations in specialized HF units.
Predictive Role of Admission Venous Lactate Level in Patients with Upper Gastrointestinal Bleeding: A Prospective Observational Study
Authors: Strzałka Marcin , Winiarski Marek , Dembiński Marcin , Pędziwiatr Michał , Matyja Andrzej , Kukla Michał
Published: January 2022

Upper gastrointestinal bleeding (UGIB) is one of the most common emergencies. Risk stratification is essential in patients with this potentially life-threatening condition. The aim of this prospective study was to evaluate the usefulness of the admission venous lactate level in predicting clinical outcomes in patients with UGIB. All consecutive adult patients hospitalized due to UGIB were included in the study. The clinical data included the demographic characteristics of the observed population, etiology of UGIB, need for surgical intervention and intensive care, bleeding recurrence, and mortality rates. Venous lactate was measured in all patients on admission. Logistic regression analyses were used to calculate the odds ratios (OR) of lactate levels for all outcomes. The receiver operating characteristic (ROC) curve was used to determine the accuracy of lactate levels in measuring clinical outcomes, while Youden index was used to calculate the best cut-off points. A total of 221 patients were included in the study (151M; 70F). There were 24 cases of UGIB recurrence (10.8%), 19 patients (8.6%) required surgery, and 37 individuals (16.7%) required intensive care. Mortality rate was 11.3% (25 cases). The logistic regression analysis showed statistically significant association between admission venous lactate and all clinical outcomes: mortality (OR = 1.39, 95%CI: 1.22–1.58, p < 0.001), recurrence of bleeding (OR = 1.16, 95%CI: 1.06; 1.28, p = 0.002), surgical intervention (OR = 1.17, 95%CI: 1.06–1.3, p = 0.002) and intensive care (OR = 1.33, 95%CI: 1.19–1.5, p < 0.001). The ROC curve analysis showed a high predictive value of lactate levels for all outcomes, especially mortality: cut-off point 4.3 (AUC = 0.82, 95%CI: 0.72–0.92, p < 0.001) and intensive care: cut-off point 4.2 (AUC = 0.76, 95%CI: 0.66–0.85, p < 0.001). Admission venous lactate level may be a useful predictive factor of clinical outcomes in patients with UGIB.
CDCP1 Expression Is a Potential Biomarker of Poor Prognosis in Resected Stage I Non-Small-Cell Lung Cancer
Authors: Nam Yunha , Choi Chang-Min , Park Young Soo , Jung HyunA , Hwang Hee Sang , Lee Jae Cheol , Lee Jung Wook , Lee Jung Eun , Kang Jung Hee , Jung Byung Hun , Ji Wonjun
Published: January 2022

Background: Although early-stage lung cancer has increased owing to the introduction of screening programs, high recurrence rate remains a critical concern. We aimed to explore biomarkers related to the prognosis of surgically resected non-small-cell lung cancer (NSCLC). Methods: In this retrospective study, we collected medical records of patients with NSCLC and matched tissue microarray blocks from surgical specimens. Semiquantitative immunohistochemistry was performed for measuring the expression level of fibroblast activation protein-alpha (FAP-α), Jagged-1 (JAG1), and CUB-domain-containing protein 1 (CDCP1). Results: A total of 453 patients who underwent complete resection between January 2011 and February 2012 were enrolled; 55.2% patients had stage I NSCLC, and 31.1% presented squamous cell carcinoma. Disease stage was a significant risk factor for recurrence and death, and age ≥ 65 years and male sex were associated with poor overall survival. FAP-a and JaG1 were not related to survivals, while CDCP1-expressing patients exhibited poor disease-free and overall survival. Moreover, CDCP1 expression in stage I NSCLC was significantly associated with recurrence. Conclusions: Old age, male sex, and high pathological stage were poor prognostic factors in patients with NSCLC who underwent surgical resection. Furthermore, CDCP1 expression could serve as a biomarker for poor prognosis in stage I NSCLC.
Thermal Qualification of the UHTCMCs Produced Using RF-CVI Technique with VMK Facility at DLR
Authors: Venkatachalam Vinothini , Blem Sergej , Gülhan Ali , Binner Jon
Published: January 2022

Ultra high-temperature ceramic matrix composites (UHTCMCs) based on carbon fibre (Cf) have been shown to offer excellent temperature stability exceeding 2000 °C in highly corrosive environments, which are prime requirements for various aerospace applications. In C3Harme, a recent European Union-funded Horizon 2020 project, an experimental campaign has been carried out to assess and screen a range of UHTCMC materials for near-zero ablation rocket nozzle and thermal protection systems. Samples with ZrB2-impregnated pyrolytic carbon matrices and 2.5D woven continuous carbon fibre preforms, produced by slurry impregnation and radio frequency aided chemical vapour infiltration (RF-CVI), were tested using the vertical free jet facility at DLR, Cologne using solid propellants. When compared to standard CVI, RFCVI accelerates pyrolytic carbon densification, resulting in a much shorter manufacturing time. The samples survived the initial thermal shock and subsequent surface temperatures of >2000 °C with a minimal ablation rate. Post-test characterisation revealed a correlation between surface temperature and an accelerated catalytic activity, which lead to an understanding of the crucial role of preserving the bulk of the sample.
Modelling the Heating Process in the Transient and Steady State of an In Situ Tape-Laying Machine Head
Authors: de Sá Rodrigues Jhonny , Gonçalves Paulo Teixeira , Pina Luis , Gomes de Almeida Fernando
Published: January 2022

As the use of composite materials increases, the search for suitable automated processes gains relevance for guaranteeing production quality by ensuring the uniformity of the process, minimizing the amount of scrap generated, and reducing the time and energy consumption. Limitations on production by traditional means such as hand lay-up, vacuum bagging, and in-autoclave methods tend not to be as efficient when the size and shape complexity of the part being produced increases, motivating the search for alternative processes such as automated tape laying (ATL). This work aims to describe the process of modelling and simulating a composite ATL with in situ consolidation by characterizing the machine elements and using the finite differences method in conjunction with energy balances in order to create a digital twin of the process for further control design. The modelling approach implemented is able to follow the process dynamics when changes are made to the heating element and to predict the composite material temperature response, making it suitable for use as a digital twin of a production process using an ATL machine.
Characterization of Wire-Bonding on LDS Materials and HF-PCBs for High-Frequency Applications
Authors: Guenther Thomas , Werum Kai , Müller Ernst , Wolf Marius , Zimmermann André
Published: January 2022

Thermosonic wire bonding is a well-established process. However, when working on advanced substrate materials and the associated required metallization processes to realize innovative applications, multiple factors impede the straightforward utilization of the known process. Most prominently, the surface roughness was investigated regarding bond quality in the past. The practical application of wire bonding on difficult-to-bond substrates showed inhomogeneous results regarding this quality characteristic. This study describes investigations on the correlation among the surface roughness, profile peak density and bonding quality of Au wire bonds on thermoplastic and thermoset-based substrates used for high-frequency (HF) applications and other high-end applications. FR4 PCB (printed circuit board flame resitant class 4) were used as references and compared to HF-PCBs based on thermoset substrates with glass fabric and ceramic filler as well as technical thermoplastic materials qualified for laser direct structuring (LDS), namely LCP (liquid crystal polymer), PEEK (polyether ether ketone) and PTFE (polytetrafluoroethylene). These LDS materials for HF applications were metallized using autocatalytic metal deposition to enable three-dimensional structuring, eventually. For that purpose, bond parameters were investigated on the mentioned test substrates and compared with state-of-the-art wire bonding on FR4 substrates as used for HF applications. Due to the challenges of the limited thermal conductivity and softening of such materials under thermal load, the surface temperatures were matched up by thermography and the adaptation of thermal input. Pull tests were carried out to determine the bond quality with regard to surface roughness. Furthermore, strategies to increase reliability by the stitch-on-ball method were successfully applied.
Investigations upon the Improvement of Dermatophyte Identification Using an Online Mass Spectrometry Application
Authors: Jabet Arnaud , Normand Anne-Cécile , Moreno-Sabater Alicia , Guillot Jacques , Risco-Castillo Veronica , Brun Sophie , Demar Magalie , Blaizot Romain , Nabet Cécile , Packeu Ann , Piarroux Renaud
Published: January 2022

Online MALDI-TOF mass spectrometry applications, such as MSI-2, have been shown to help identify dermatophytes, but recurrent errors are still observed between phylogenetically close species. The objective of this study was to assess different approaches to reduce the occurrence of such errors by adding new reference spectra to the MSI-2 application. Nine libraries were set up, comprising an increasing number of spectra obtained from reference strains that were submitted to various culture durations on two distinct culture media: Sabouraud gentamicin chloramphenicol medium and IDFP Conidia medium. The final library included spectra from 111 strains of 20 species obtained from cultures on both media collected every three days after the appearance of the colony. The performance of each library was then analyzed using a cross-validation approach. The spectra acquisitions were carried out using a Microflex Bruker spectrometer. Diversifying the references and adding spectra from various culture media and culture durations improved identification performance. The percentage of correct identification at the species level rose from 63.4 to 91.7% when combining all approaches. Nevertheless, residual confusion between close species, such as Trichophyton rubrum, Trichophyton violaceum and Trichophyton soudanense, remained. To distinguish between these species, mass spectrometry identification should take into account basic morphological and/or clinico-epidemiological features.
Time Evolution of Market Efficiency and Multifractality of the Japanese Stock Market
Authors: Takaishi Tetsuya
Published: January 2022

This study investigates the time evolution of market efficiency in the Japanese stock markets, considering three indices: Tokyo Stock Price Index (TOPIX), Tokyo Stock Exchange Second Section Index, and TOPIX-Small. The Hurst exponent reveals that the Japanese markets are inefficient in their early stages and improve gradually. TOPIX and TOPIX-Small showed an anti-persistence around the year 2000, which still persists. The degree of multifractality varies over time and does not show that the Japanese markets are permanently efficient. The multifractal properties of the Japanese markets changed considerably around the year 2000; this may have been caused by the complete migration from the stock trading floor to the Tokyo Stock Exchange’s computer trading system and the financial system reform, also known as the “Japanese Big Bang”.
Global Trends in Urban Agriculture Research: A Pathway toward Urban Resilience and Sustainability
Authors: Yan Dan , Liu Litao , Liu Xiaojie , Zhang Ming
Published: January 2022

Urban agriculture has been proposed as an important urban element to deal with the challenges of food insecurity and environmental deterioration. In order to track current popular topics and global research trends in urban agriculture, we used bibliometric analysis and visualization mapping to evaluate and analyze the developments in the knowledge of urban agriculture based on 605 papers from the core collection database Web of Science from 2001–2021. The results were as follows. (1) The number of urban agriculture publications increased substantially year by year, indicating that the field is attracting increasing attention. The University of Kassel, Chinese Academy of Sciences, and University of Freiburg are the most productive research institutions in the field of urban agriculture. The top-five most influential countries are the Unites States, Germany, the United Kingdom, Italy, and China, of which the Unites States plays a central role in the cooperative linkage between countries. (2) Research on urban agriculture focuses not only on food production and different styles but also on how to realize the various functions of urban agriculture. In addition, UA-related sustainability and the water-energy-food nexus have become two emerging research topics. (3) Urban agriculture does not necessarily mean a resource-conserving and environmentally friendly food system. To achieve sustainable development, a transition based on technological innovation is needed. How to improve the sustainable development level of the food system while fully considering the resilience, sustainability, and versatility of urban agriculture is the main direction of future research.
AntiGan: An Epinutraceutical Bioproduct with Antitumor Properties in Cultured Cell Lines
Authors: Martínez-Iglesias Olaia , Carrera Ivan , Naidoo Vinogran , Cacabelos Ramón
Published: January 2022

Novel and effective chemotherapeutic agents are needed to improve cancer treatment. Epidrugs are currently used for cancer therapy but also exhibit toxicity. Targeting the epigenetic apparatus with bioproducts may aid cancer prevention and treatment. To determine whether the lipoprotein marine extract AntiGan shows epigenetic and antitumor effects, cultured HepG2 (hepatocellular carcinoma) and HCT116 (colorectal carcinoma) cell lines were treated with AntiGan (10, 50, 100, and to 500 µg/mL) for 24 h, 48 h, and 72 h. AntiGan (10 µg/mL) reduced cell viability after 48 h and increased Bax expression; AntiGan (10 and 50 µg/mL) increased caspase-3 immunoreactivity in HepG2 and HCT116 cells. AntiGan (10 and 50 µg/mL) attenuated COX-2 and IL-17 expression in both cell lines. AntiGan (10 µg/mL) increased 5mC levels in both cell types and reduced DNMT1 and DNMT3a expression in these cells. AntiGan (10 and 50 µg/mL) promoted DNMT3a immunoreactivity and reduced SIRT1 mRNA expression in both cell types. In HCT116 cells treated with AntiGan (10 µg/mL), SIRT1 immunoreactivity localized to nuclei and the cytoplasm; AntiGan (50 µg/mL) increased cytoplasmic SIRT1 localization in HCT116 cells. AntiGan is a novel antitumoral bioproduct with epigenetic properties (epinutraceutical) for treating liver and colorectal cancer.
Photobiomodulation for the Treatment of Primary Headache: Systematic Review of Randomized Clinical Trials
Authors: Gomes Andréa Oliver , Martimbianco Ana Luiza Cabrera , Brugnera Junior Aldo , Horliana Anna Carolina Ratto Tempestini , da Silva Tamiris , Santos Elaine Marcílio , Fragoso Yara Dadalti , Fernandes Kristianne Porta Santos , Nammour Samir , Bussadori Sandra Kalil
Published: January 2022

The purpose of this study was to evaluate the efficacy and safety of photobiomodulation as an adjuvant treatment for primary headache. A systematic review of randomized clinical trials was performed. For such, electronic searches were performed in the MEDLINE, Embase, Cochrane Library, LILACS, PEDro, PsycInfo, Clinicaltrials.gov., and WHO/ICTRP databases, with no restrictions imposed regarding language or year of publication. We included studies that assessed any photobiomodulation therapy as an adjuvant treatment for primary headache compared to sham treatment, no treatment, or another intervention. The methodological assessment was conducted using the Cochrane Risk of Bias tool. The certainty of the evidence was classified using the GRADE approach. Four randomized clinical trials were included. Most of the included studies had an overall high risk of bias. Compared to sham treatment, photobiomodulation had a clinically important effect on pain in individuals with primary headache. Despite the benefits reported for other outcomes, the estimates were imprecise, and the certainty of the evidence was graded as low. These findings are considered insufficient to support the use of photobiomodulation in the treatment of primary headache. Randomized clinical trials, with higher methodological quality, are needed to enhance the reliability of the estimated effects.
Composing Vessel Fleets for Maintenance at Offshore Wind Farms by Solving a Dual-Level Stochastic Programming Problem Using GRASP
Authors: Bolstad Kamilla Hamre , Joshi Manu , Hvattum Lars Magnus , Stålhane Magnus
Published: January 2022

Background: Dual-level stochastic programming is a technique that allows modelling uncertainty at two different levels, even when the time granularity differs vastly between the levels. In this paper we study the problem of determining the optimal fleet size and mix of vessels performing maintenance operations at offshore wind farms. In this problem the strategic planning spans decades, while operational planning is performed on a day-to-day basis. Since the operational planning level must somehow be taken into account when making strategic plans, and since uncertainty is present at both levels, dual-level stochastic programming is suitable. Methods: We present a heuristic solution method for the problem based on the greedy randomized adaptive search procedure (GRASP). To evaluate the operational costs of a given fleet, a novel fleet deployment heuristic (FDH) is embedded into the GRASP. Results: Computational experiments show that the FDH produces near optimal solutions to the operational day-to-day fleet deployment problem. Comparing the GRASP to exact methods, it produces near optimal solutions for small instances, while significantly improving the primal solutions for larger instances, where the exact methods do not converge. Conclusions: The proposed heuristic is suitable for solving realistic instances, and produces near optimal solution in less than 2 h.
A Study on Bearing Dynamic Features under the Condition of Multiball–Cage Collision
Authors: Ma Shuaijun , Zhang Xiaohong , Yan Ke , Zhu Yongsheng , Hong Jun
Published: January 2022

Cage stability directly affects the dynamic performance of rolling bearing, which, in turn, affects the operating state of rotating equipment. The random collision between the rolling elements and the cage pocket is the main reason for cage instability. In this paper, from the perspective of the relative sliding velocity between the rolling elements and the bearing raceway, the interactions of the rolling elements and the cage pockets were analyzed, and the four zones with different collision features were defined. On this basis, and on the basis of the bearing dynamics model, the interaction of two adjacent rolling elements and the cage pockets in the a’–b’ area is discussed, and the peak impact force of the adjacent two balls and the cage pockets was investigated in terms of the rotation speed, radial load, acceleration/deceleration, and materials. When the ball runs close to the loaded zone, the probability of multiball random collision increases, which leads to an increase in the cage instability. At the entrance of the loaded zone, the peak impact force has the greatest impact on the cage stability during the acceleration process. Compared to the radial load applied to the bearing, the peak impact force is more sensitive to the bearing speed changes. The multiball collision analysis method provides a new idea for the research of cage stability.
Assessment of Soft Computing Techniques for the Prediction of Compressive Strength of Bacterial Concrete
Authors: Almohammed Fadi , Sihag Parveen , Sammen Saad Sh. , Ostrowski Krzysztof Adam , Singh Karan , Prasad C. Venkata Siva Rama , Zajdel Paulina
Published: January 2022

In this investigation, the potential of M5P, Random Tree (RT), Reduced Error Pruning Tree (REP Tree), Random Forest (RF), and Support Vector Regression (SVR) techniques have been evaluated and compared with the multiple linear regression-based model (MLR) to be used for prediction of the compressive strength of bacterial concrete. For this purpose, 128 experimental observations have been collected. The total data set has been divided into two segments such as training (87 observations) and testing (41 observations). The process of data set separation was arbitrary. Cement, Aggregate, Sand, Water to Cement Ratio, Curing time, Percentage of Bacteria, and type of sand were the input variables, whereas the compressive strength of bacterial concrete has been considered as the final target. Seven performance evaluation indices such as Correlation Coefficient (CC), Coefficient of determination (R2), Mean Absolute Error (MAE), Root Mean Square Error (RMSE), Bias, Nash-Sutcliffe Efficiency (NSE), and Scatter Index (SI) have been used to evaluate the performance of the developed models. Outcomes of performance evaluation indices recommend that the Polynomial kernel function based SVR model works better than other developed models with CC values as 0.9919, 0.9901, R2 values as 0.9839, 0.9803, NSE values as 0.9832, 0.9800, and lower values of RMSE are 1.5680, 1.9384, MAE is 0.7854, 1.5155, Bias are 0.2353, 0.1350 and SI are 0.0347, 0.0414 for training and testing stages, respectively. The sensitivity investigation shows that the curing time (T) is the vital input variable affecting the prediction of the compressive strength of bacterial concrete, using this data set.
Ag2O versus Cu2O in the Catalytic Isomerization of Coordinated Diaminocarbenes to Formamidines: A Theoretical Study
Authors: Van der Maelen Juan F. , Ruiz Javier
Published: January 2022

DFT theoretical calculations for the Ag2O-induced isomerization process of diaminocarbenes to formamidines, coordinated to Mn(I), have been carried out. The reaction mechanism found involves metalation of an N-H residue of the carbene ligand by the catalyst Ag2O and the formation of a key transition state showing a μ-η2:η2 coordination of the formamidinyl ligand between manganese and silver, which allows a translocation process of Mn(I) and silver(I) ions between the carbene carbon atom and the nitrogen atom, before the formation of the formamidine ligand is completed. Calculations carried out using Cu2O as a catalyst instead of Ag2O show a similar reaction mechanism that is thermodynamically possible, but highly unfavorable kinetically and very unlikely to be observed, which fully agrees with experimental results.
Where ppm Quantities of Silsesquioxanes Make a Difference—Silanes and Cage Siloxanes as TiO2 Dispersants and Stabilizers for Pigmented Epoxy Resins
Authors: Brząkalski Dariusz , Przekop Robert E. , Frydrych Miłosz , Pakuła Daria , Dobrosielska Marta , Sztorch Bogna , Marciniec Bogdan
Published: January 2022

In this work, silsesquioxane and spherosilicate compounds were assessed as novel organosilicon coupling agents for surface modification of TiO2 in a green process, and compared with their conventional silane counterparts. The surface-treated TiO2 particles were then applied in preparation of epoxy (EP) composites and the aspects of pigment dispersion, suspension stability, hiding power, as well as the composite mechanical and thermal properties were discussed. The studied compounds loading was between 0.005–0.015% (50–150 ppm) in respect to the bulk composite mass and resulted in increase of suspension stability and hiding power by over an order of magnitude. It was found that these compounds may be an effective alternative for silane coupling agents, yet due to their low cost and simplicity of production and manipulation, silanes and siloxanes are still the most straight-forward options available. Nonetheless, the obtained findings might encourage tuning of silsesquioxane compounds structure and probably process itself if implementation of these novel organosilicon compounds as surface treatment agents is sought for special applications, e.g., high performance coating systems.
Organic Nanoparticles Based on D-A-D Small Molecule: Self-Assembly, Photophysical Properties, and Synergistic Photodynamic/Photothermal Effects
Authors: Yue Liangliang , Li Haolan , Sun Qi , Luo Xiaogang , Wu Fengshou , Zhu Xunjin
Published: January 2022

Cancer is one of the major diseases threatening human health. Traditional cancer treatments have notable side-effects as they can damage the immune system. Recently, phototherapy, as a potential strategy for clinical cancer therapy, has received wide attention due to its minimal invasiveness and high efficiency. Herein, a small organic molecule (PTA) with a D-A-D structure was prepared via a Sonogashira coupling reaction between the electron-withdrawing dibromo-perylenediimide and electron-donating 4-ethynyl-N,N-diphenylaniline. The amphiphilic organic molecule was then transformed into nanoparticles (PTA-NPs) through the self-assembling method. Upon laser irradiation at 635 nm, PTA-NPs displayed a high photothermal conversion efficiency (PCE = 43%) together with efficient reactive oxygen species (ROS) generation. The fluorescence images also indicated the production of ROS in cancer cells with PTA-NPs. In addition, the biocompatibility and photocytotoxicity of PTA-NPs were evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and live/dead cell co-staining test. Therefore, the as-prepared organic nanomaterials were demonstrated as promising nanomaterials for cancer phototherapy in the clinic.
The Effect of Commercially Available Desensitizers on Bond Strength Following Cementation of Zirconia Crowns Using Self-Adhesive Resin Cement—An In Vitro Study
Authors: Dewan Harisha , Sayed Mohammed E. , Alqahtani Nasser M. , Alnajai Tariq , Qasir Abdulaziz , Chohan Hitesh
Published: January 2022

The improvement of the tensile strength of zirconia crowns after the application of commercially available desensitizers can provide added advantages for the durability and strength of zirconia prostheses. We assessed the retention of zirconia crowns when Gluma, Shield Force Plus, and Telio CS desensitizers were used with resin luting cement. Four groups with ten specimens each (n = 10) were considered as Group 1 (Control group, with no desensitizer application before crown cementation with resin cement) and Groups 2, 3, and 4 (with a single coat of Gluma dentin desensitizer, Telio CS desensitizer, or Shield Force Plus desensitizer applied before crown cementation, respectively). Thermocycling was then carried out, and each group was tested to determine the associated retentive forces and type of failure. The data were statistically analyzed, which showed that the mean tensile-strength values were significantly higher in Group 2 (p-value = 0.001), Group 3 (p-value = 0.027), and Group 4 (p-value = 0.014), when compared with the Control group. Clinicians should consider the application of any of these three desensitizers, as they can successfully abate dentin hypersensitivity after tooth preparation, as well as increase the durability and strength of the zirconia prosthesis.
Starch as the Flame Retardant for Electrolytes in Lithium-Ion Cells
Authors: Pigłowska Marita , Kurc Beata , Rymaniak Łukasz
Published: January 2022

The main purpose of this work is to illustrate the flame retardant properties of corn starch that is used as an additive to the classic electrolytes in lithium-ion cells. The advantages of using natural biomass include the increased biodegradability of the cell, compliance with the slogan of green chemistry, as well as the widespread availability and easy isolation of this ingredient. Due to the non-Newtonian properties of starch, it increases work safety and prevents the occurrence of thermal runaway as a shear-thinning fluid in the event of a collision. Thus, its use may, in the future, prevent explosions that affect electric cars with lithium-ion batteries without significantly degrading the electrochemical parameters of the cell. In the manuscript, the viscosity test, flash point measurements, the SET (self-extinguishing time) test and conductivity measurements were performed, in addition to the determination of electrochemical impedance spectroscopy (EIS) for the anode system. Additionally, the kinetic and thermodynamic parameters, for both flow and conductivity, were determined for a deeper analysis; this constitutes the scientific novelty of this study. Through mathematical analysis, it was shown that the optimal amount of added starch is 5%. This is supported primarily by the determined kinetic and thermodynamic parameters and the fact that the system did not gel during heating.