bluuebunny/crossref_metadata_2025 huggingface dataset:
Real-Time Wave Simulation of Large-Scale Open Sea Based on Self-Adaptive Filtering and Screen Space Level of Detail
Authors: Duan Xi , Liu Jian , Wang Xinjie
Published: March 2024

The real-time simulation technology of large-scale open sea surfaces has been of great importance in fields such as computer graphics, ocean engineering, and national security. However, existing technologies typically have performance requirements or platform limitations, and the two are often impossible to balance. Based on the camera-view-based screen space level of detail strategy and virtual camera pose adaptive filtering strategy proposed in this article, we have developed a fast and comprehensive solution for rendering large-scale open sea surfaces. This solution is designed to work without the need for special hardware extensions, making it easy to deploy across various platforms. Additionally, it enhances the degrees of freedom of virtual camera movement. After conducting performance tests under various camera poses, our filtering strategy was found to be effective. Notably, the time cost of simulation using 60 waves at the height of 6 m above sea level was only 0.184 ms. In addition, we conducted comparative experiments with four state-of-the-art algorithms currently in use, and our solution outperformed the others with the best performance and suboptimal visual effects. These results demonstrate the superiority of our approach in terms of both efficiency and effectiveness.
Experimental Analysis of the Influence of the Cross-Section Coefficient on the Ship Squat While the Ship Exits the Shiplift Chamber
Authors: Fu Luzhidan , Hu Yaan , Li Zhonghua
Published: March 2024

Due to the small cross-section coefficient of a shiplift chamber, the hydraulics problem becomes a complex water flow problem in ship operation. To prevent the ship from touching the bottom and ensure the safety of ship navigation and the docking of the chamber, a generalized physical model was used to study the ship squat of a 1000 t ship leaving the chamber. Throughout the experiment, the law of variation law in the ship squat under different chamber widths, water depths, chamber lengths, ship speeds and ship starting acceleration was obtained. In this study, the flow pattern of the ship leaving the chamber was divided into two parts: backflow and bottom flow. According to sensitivity analysis, when the shipping speed was less than 0.9 m/s, the influence of the width of the chamber on the squat was greater than that of the depth of the chamber; when the speed is greater than 0.9 m/s, the influence of the depth of the chamber on the squat was slightly greater than that of the width of the chamber. Based on our theoretical analysis, the basic expression of a ship squat was established.
Redundant and Distinct Roles of Two 14-3-3 Proteins in Fusarium sacchari, Pathogen of Sugarcane Pokkah Boeng Disease
Authors: Chen Yuejia , Yao Ziting , Zhao Lixian , Yu Mei , Chen Baoshan , Zou Chengwu
Published: March 2024

Fusarium sacchari, a key pathogen of sugarcane, is responsible for the Pokkah boeng disease (PBD) in China. The 14-3-3 proteins have been implicated in critical developmental processes, including dimorphic transition, signal transduction, and carbon metabolism in various phytopathogenic fungi. However, their roles are poorly understood in F. sacchari. This study focused on the characterization of two 14-3-3 protein-encoding genes, FsBmh1 and FsBmh2, within F. sacchari. Both genes were found to be expressed during the vegetative growth stage, yet FsBmh1 was repressed at the sporulation stage in vitro. To elucidate the functions of these genes, the deletion mutants ΔFsBmh1 and ΔFsBmh2 were generated. The ΔFsBmh2 exhibited more pronounced phenotypic defects, such as impaired hyphal branching, septation, conidiation, spore germination, and colony growth, compared to the ΔFsBmh1. Notably, both knockout mutants showed a reduction in virulence, with transcriptome analysis revealing changes associated with the observed phenotypes. To further investigate the functional interplay between FsBmh1 and FsBmh2, we constructed and analyzed mutants with combined deletion and silencing (ΔFsBmh/siFsBmh) as well as overexpression (O-FsBmh). The combinations of ΔFsBmh1/siFsBmh2 or ΔFsBmh2/siFsBmh1 displayed more severe phenotypes than those with single allele deletions, suggesting a functional redundancy between the two 14-3-3 proteins. Yeast two-hybrid (Y2H) assays identified 20 proteins with pivotal roles in primary metabolism or diverse biological functions, 12 of which interacted with both FsBmh1 and FsBmh2. Three proteins were specifically associated with FsBmh1, while five interacted exclusively with FsBmh2. In summary, this research provides novel insights into the roles of FsBmh1 and FsBmh2 in F. sacchari and highlights potential targets for PBD management through the modulation of FsBmh functions.
A Comprehensive Evaluation of AI-Assisted Diagnostic Tools in ENT Medicine: Insights and Perspectives from Healthcare Professionals
Authors: Alshehri Sarah , Alahmari Khalid A. , Alasiry Areej
Published: March 2024

The integration of Artificial Intelligence (AI) into healthcare has the potential to revolutionize medical diagnostics, particularly in specialized fields such as Ear, Nose, and Throat (ENT) medicine. However, the successful adoption of AI-assisted diagnostic tools in ENT practice depends on the understanding of various factors; these include influences on their effectiveness and acceptance among healthcare professionals. This cross-sectional study aimed to assess the usability and integration of AI tools in ENT practice, determine the clinical impact and accuracy of AI-assisted diagnostics in ENT, measure the trust and confidence of ENT professionals in AI tools, gauge the overall satisfaction and outlook on the future of AI in ENT diagnostics, and identify challenges, limitations, and areas for improvement in AI-assisted ENT diagnostics. A structured online questionnaire was distributed to 600 certified ENT professionals with at least one year of experience in the field. The questionnaire assessed participants’ familiarity with AI tools, usability, clinical impact, trust, satisfaction, and identified challenges. A total of 458 respondents completed the questionnaire, resulting in a response rate of 91.7%. The majority of respondents reported familiarity with AI tools (60.7%) and perceived them as generally usable and clinically impactful. However, challenges such as integration with existing systems, user-friendliness, accuracy, and cost were identified. Trust and satisfaction levels varied among participants, with concerns regarding data privacy and support. Geographic and practice setting differences influenced perceptions and experiences. The study highlights the diverse perceptions and experiences of ENT professionals regarding AI-assisted diagnostics. While there is general enthusiasm for these tools, challenges related to integration, usability, trust, and cost need to be addressed for their widespread adoption. These findings provide valuable insights for developers, policymakers, and healthcare providers aiming to enhance the role of AI in ENT practice.
Associations between Chronic Kidney Disease and Migraine Incidence: Findings from a Korean Longitudinal Big Data Study
Authors: Kwon Mi Jung , Kim Jwa-Kyung , Kim Min-Jeong , Yoo Dae Myoung , Lee Na-Eun , Han Kyeong Min , Kim Nan Young , Kang Ho Suk , Choi Hyo Geun , Kim Eun Soo
Published: March 2024

While headaches frequently occur in individuals with chronic kidney disease (CKD), there are few statistical evaluations of their connection to migraines in population-based studies. In this nationwide longitudinal follow-up study of Korean health examination data (2002–2019), a total of 15,443 participants with CKD and 61,772 matched controls were enrolled. We applied overlap-weighted Cox proportional hazard regression models to assess hazard ratios, examining the correlation between CKD and the development of migraines. After accounting for various factors, we observed a modest reduction of approximately 11% in the likelihood of migraine occurrence among CKD patients (95% confidence intervals = 0.81–0.97) during the 16-year monitoring period. Subgroup analysis revealed a significant association among specific demographic and health conditions, including individuals aged 70 or older, females, overweight individuals, nonsmokers, and those without hypertension or diabetes. Our research may indicate a potential relationship between CKD and the onset of migraines in Korean adults, suggesting a slight reduction in the probability of the occurrence of migraines among those with CKD. These findings emphasize the need for attentive follow-up and preventive management in individuals without the identified protective factors, particularly in male CKD patients under the age of 70 with hypertension.
Pathophysiology and Clinical Management of Dyslipidemia in People Living with HIV: Sailing through Rough Seas
Authors: Papantoniou Eleni , Arvanitakis Konstantinos , Markakis Konstantinos , Papadakos Stavros P. , Tsachouridou Olga , Popovic Djordje S. , Germanidis Georgios , Koufakis Theocharis , Kotsa Kalliopi
Published: March 2024

Infections with human immunodeficiency virus (HIV) and acquired immune deficiency syndrome (AIDS) represent one of the greatest health burdens worldwide. The complex pathophysiological pathways that link highly active antiretroviral therapy (HAART) and HIV infection per se with dyslipidemia make the management of lipid disorders and the subsequent increase in cardiovascular risk essential for the treatment of people living with HIV (PLHIV). Amongst HAART regimens, darunavir and atazanavir, tenofovir disoproxil fumarate, nevirapine, rilpivirine, and especially integrase inhibitors have demonstrated the most favorable lipid profile, emerging as sustainable options in HAART substitution. To this day, statins remain the cornerstone pharmacotherapy for dyslipidemia in PLHIV, although important drug–drug interactions with different HAART agents should be taken into account upon treatment initiation. For those intolerant or not meeting therapeutic goals, the addition of ezetimibe, PCSK9, bempedoic acid, fibrates, or fish oils should also be considered. This review summarizes the current literature on the multifactorial etiology and intricate pathophysiology of hyperlipidemia in PLHIV, with an emphasis on the role of different HAART agents, while also providing valuable insights into potential switching strategies and therapeutic options.
Ablative Treatments for Small Renal Masses and Management of Recurrences: A Comprehensive Review
Authors: Aveta Achille , Iossa Vincenzo , Spena Gianluca , Conforti Paolo , Pagano Giovanni , Dinacci Fabrizio , Verze Paolo , Manfredi Celeste , Ferro Matteo , Lasorsa Francesco , Spirito Lorenzo , Napolitano Luigi , Tufano Antonio , Fiorenza Alessandra , Russo Pierluigi , Crocerossa Fabio , Lucarelli Giuseppe , Perdonà Sisto , Sanseverino Roberto , Siracusano Salvatore , Cilio Simone , Pandolfo Savio Domenico
Published: March 2024

This review focuses on ablative techniques for small renal masses (SRMs), including radiofrequency ablation (RFA), cryoablation (CA), microwave ablation (MWA), and irreversible electroporation (IRE), and discusses recurrence management. Through an extensive literature review, we outline the procedures, outcomes, and follow-up strategies associated with each ablative method. The review provides a detailed examination of these techniques—RFA, CA, MWA, and IRE—elucidating their respective outcomes. Recurrence rates vary among them, with RFA and CA showing comparable rates, MWA demonstrating favorable short-term results, and IRE exhibiting promise in experimental stages. For managing recurrences, various strategies are considered, including active surveillance, re-ablation, or salvage surgery. Surveillance is preferred post-RFA and post-CA, due to slow SRM growth, while re-ablation, particularly with RFA and CA, is deemed feasible without additional complications. Salvage surgery emerges as a viable option for larger or resistant tumors. While ablative techniques offer short-term results comparable to surgery, further research is essential to understand their long-term effects fully. Decisions concerning recurrence management should consider individual and tumor-specific factors. Imaging, notably contrast-enhanced ultrasounds, plays a pivotal role in assessing treatment success, emphasizing the necessity of a multidisciplinary approach for optimal outcomes. The lack of randomized trials highlights the need for further research.
Endovascular Drug Delivery
Authors: Lungu Claudiu N. , Creteanu Andreea , Mehedinti Mihaela C.
Published: March 2024

Drug-eluting stents (DES) and balloons revolutionize atherosclerosis treatment by targeting hyperplastic tissue responses through effective local drug delivery strategies. This review examines approved and emerging endovascular devices, discussing drug release mechanisms and their impacts on arterial drug distribution. It emphasizes the crucial role of drug delivery in modern cardiovascular care and highlights how device technologies influence vascular behavior based on lesion morphology. The future holds promise for lesion-specific treatments, particularly in the superficial femoral artery, with recent CE-marked devices showing encouraging results. Exciting strategies and new patents focus on local drug delivery to prevent restenosis, shaping the future of interventional outcomes. In summary, as we navigate the ever-evolving landscape of cardiovascular intervention, it becomes increasingly evident that the future lies in tailoring treatments to the specific characteristics of each lesion. By leveraging cutting-edge technologies and harnessing the potential of localized drug delivery, we stand poised to usher in a new era of precision medicine in vascular intervention.
(E)-1-(4-Methoxyphenyl)-5-methyl-4-(1-phenyl-4-((2-(2,4,6-trichlorophenyl)hydrazineylidene)methyl)-1H-pyrazol-3-yl)-1H-1,2,3-triazole
Authors: Abdel-Wahab Bakr F. , Mohamed Hanan A. , Kariuki Benson M. , El-Hiti Gamal A.
Published: March 2024

The reaction of equimolar quantities of 3-(1-(4-methoxyphenyl)-5-methyl-1H-1,2,3-triazol-4-yl)-1-phenyl-1H-pyrazole-4-carbaldehyde and (2,4,6-trichlorophenyl)hydrazine in ethanol containing concentrated hydrochloric acid (0.2 mL; 37%) as a catalyst under reflux for 2 h yielded 1-(1-(benzofuran-2-yl)ethylidene)-2-(2,4,6-trichlorophenyl)hydrazine. The crude produced was purified by crystallization using dimethylformamide to provide the title heterocycle in a 95% yield. The structure of the newly synthesized heterocycle was confirmed through X-ray diffraction and spectral analyses.
Investigation of the Effect of Stress on Oxygen Diffusion in Pure Titanium Using a Phase-Field Model
Authors: Wang Yaomian , Zhang Mengqi , Yang Huanping
Published: March 2024

Diffusion plays a vital role during the fabrication of many materials. It is a well-known fact that stress can influence diffusion behavior. In order to optimize material processing techniques, a quantitative evaluation of the effect of stress on diffusion is essentially required. By analyzing the free energy change in a Ti-O system during diffusion, a phase-field model was developed to address this issue. Using this model, the diffusion of oxygen atoms in pure titanium under different stress states was investigated. It was observed that the true equilibrium concentration of oxygen was proportional to its hydrostatic pressure. Tensile stress can increase the oxygen concentration. This raise in concentration decreased with temperature. However, the promotion of diffusion can be attained in deeper regions at a higher temperature. On the contrary, compressive stress inhibited the diffusion of oxygen in pure titanium. Under a certain compressive stress, the decrease in the oxygen concentration at the surface layer was more significant at a lower temperature, while a decrease could be observed at a deeper distance from the surface at a higher temperature. A thermodynamic explanation of the effect of stress on diffusion was given based on the proposed phase-field model.
Metallic and Ceramic Materials Integrity—Surface Engineering for Wear, Corrosion and Erosion Prevention
Authors: Szala Mirosław , Walczak Mariusz
Published: March 2024

The literature systematically describes the wear behavior and phenomena responsible for the degradation resistance of materials [...]
Theoretical Study of Electric, Dielectric, and Optical Properties in Ion Doped Multiferroic SrFe12O19 Nanoparticles
Authors: Apostolov Angel T. , Apostolova Iliana N. , Wesselinowa Julia Mihailowa
Published: March 2024

Electric, dielectric, and optical (band gap) properties of pure multiferroic as well as La- and Ni-doped SrFe12O19 (SFO) (at different sites) are investigated using a microscopic model and Green’s function technique. The concentration dependence of the polarization P is considered for substitution of rare earths ions on the Sr sites. For a small La ion doping concentration, x = 0.1, La-doped SFO is ferroelectric, whereas for a larger doping concentration, for example x = 0.5, it is antiferroelectric. The real part of the dielectric constant ϵ increases with an increasing magnetic field h. ϵ decreases with an increasing frequency and La dopants. Therefore, La-doped SFO is suitable for microwave application with a low dielectric constant. The magnetic properties of pure SFO NPs are also studied. Ni doping at the Fe site of SFO leads to enhanced ferroelectric polarization and dielectric constant. The band gap decreases or increases by substitution of Ni or In ions on the Fe site, respectively. The results reveal that the tuned band gap of Ni-doped SFO makes it a crucial candidate for optoelectronic and solid oxide fuel cell applications.
Towards Zero-Defect Manufacturing Based on Artificial Intelligence through the Correlation of Forces in 5-Axis Milling Process
Authors: Cascón-Morán Itxaso , Gómez Meritxell , Fernández David , Gil Del Val Alain , Alberdi Nerea , González Haizea
Published: March 2024

Zero-Defect Manufacturing (ZDM) is a promising strategy for reducing errors in industrial processes, aligned with Industry 4.0 and digitalization, aiming to carry out processes correctly the first time. ZDM relies on digital tools, notably Artificial Intelligence (AI), to predict and prevent issues at both product and process levels. This study’s goal is to significantly reduce errors in machining large parts. It utilizes data from process models and in situ monitoring for AI-driven predictions. AI algorithms anticipate part deformation based on manufacturing data. Mechanistic models simulate milling processes, calculating tool deflection from cutting forces and assessing geometric and dimensional errors. Process monitoring provides real-time data to the models during execution. The research focuses on a high-value component from the oil and gas industry, serving as a test piece to predict geometric errors in machining based on the deviation of cutting forces using AI techniques. Specifically, an AISI 1095 steel forged flange, intentionally misaligned to introduce error, undergoes multiple milling operations, including 3-axis roughing and 5-axis finishing, with 3D scans after each stage to monitor progress and deviations. The work concludes that Support Vector Machine algorithms provide accurate results for the estimation of geometric errors from the machining forces.
Polynomial Intermediate Checksum for Integrity under Releasing Unverified Plaintext and Its Application to COPA
Authors: Zhang Ping
Published: March 2024

COPA, introduced by Andreeva et al., is the first online authenticated encryption (AE) mode with nonce-misuse resistance, and it is covered in COLM, which is one of the final CAESAR portfolios. However, COPA has been proven to be insecure in the releasing unverified plaintext (RUP) setting. This paper mainly focuses on the integrity under RUP (INT-RUP) defect of COPA. Firstly, this paper revisits the INT-RUP security model for adaptive adversaries, investigates the possible factors of INT-RUP insecurity for “Encryption-Mix-Encryption”-type checksum-based AE schemes, and finds that these AE schemes with INT-RUP security vulnerabilities utilize a common poor checksum technique. Then, this paper introduces an improved checksum technique named polynomial intermediate checksum (PIC) for INT-RUP security and emphasizes that PIC is a sufficient condition for guaranteeing INT-RUP security for “Encryption-Mix-Encryption”-type checksum-based AE schemes. PIC is generated by a polynomial sum with full terms of intermediate internal states, which guarantees no information leakage. Moreover, PIC ensures the same level between the plaintext and the ciphertext, which guarantees that the adversary cannot obtain any useful information from the unverified decryption queries. Again, based on PIC, this paper proposes a modified scheme COPA-PIC to fix the INT-RUP defect of COPA. COPA-PIC is proven to be INT-RUP up to the birthday-bound security if the underlying primitive is secure. Finally, this paper discusses the properties of COPA-PIC and makes a comparison for AE modes with distinct checksum techniques. The proposed work is of good practical significance. In an interactive system where two parties communicate, the receiver can effectively determine whether the information received from the sender is valid or not, and thus perform the subsequent operation more effectively.
An Integrated Instruction and a Dynamic Fuzzy Inference System for Evaluating the Acquirement of Skills through Learning Activities by Higher Middle Education Students in Mexico
Authors: Leal-Ramírez Cecilia , Echavarría-Heras Héctor Alonso
Published: March 2024

Background: The evaluation of the development of a student’s abilities and skills through a learning activity is a topic strongly questioned by the education system in Mexico. Several instruments have been developed to achieve said evaluation. However, these involve both qualitative and subjective assessment, thereby avoiding the possibility of unambiguously verifying the development of a student’s aptitudes. Methods: We developed a new instrument composed of an integrated instruction and a dynamic fuzzy inference system. Integrated instruction is a table that contains a set of instructions and a set of indicators that make it possible to evaluate knowledge, procedure, and attitude without establishing qualitative or subjective criteria to rank them. The dynamic fuzzy inference system assesses indicators under a criterion to demonstrate the development of a student’s abilities and skills. Results: The method was applied to three different learning activities, where the assessment was precise and transparent for the student, contributing to an extraordinary identification of the acquainted knowledge, procedure, and attitude that the student displayed to develop the activity. Conclusions: Our instrument evaluates the development of abilities and skills without ambiguity or subjectivity, making efficient feedback possible and allowing it to be perfected without difficulties for future adaptations.
On Dynamics of Double-Diffusive Convection in a Rotating Couple-Stress Fluid Layer
Authors: Li Liang , Mao Yiqiu
Published: March 2024

The current article focuses on the examination of nonlinear instability and dynamic transitions in a double-diffusive rotating couple-stress fluid layer. The analysis was based on the newly developed dynamic transition theory by T. Ma and S. Wang. Through a comprehensive linear spectrum analysis and investigation of the principle of exchange of stability (PES) as the thermal Rayleigh number crosses a threshold, the nonlinear orbital changes during the transition were rigorously elucidated utilizing reduction methods. For both single real and complex eigenvalue crossings, local pitch-fork and Hopf bifurcations were discovered, and directions of these bifurcations were identified along with transition types. Furthermore, nondimensional transition numbers that signify crucial factors during the transition were calculated and the orbital structures were illustrated. Numerical studies were performed to validate the theoretical results, revealing the relations between key parameters in the system and the types of transition. The findings indicated that the presence of couple stress and a slow diffusion rate of solvent and temperature led to smoother nonlinear transitions during convection.
A Characterization of Procyclic Groups via Complete Exterior Degree
Authors: Rodrigues Bernardo G. , Russo Francesco G.
Published: March 2024

We describe the nonabelian exterior square G∧^G of a pro-p-group G (with p arbitrary prime) in terms of quotients of free pro-p-groups, providing a new method of construction of G∧^G and new structural results for G∧^G. Then, we investigate a generalization of the probability that two randomly chosen elements of G commute: this notion is known as the “complete exterior degree” of a pro-p-group and we will use it to characterize procyclic groups. Among other things, we present a new formula, which simplifies the numerical aspects which are connected with the evaluation of the complete exterior degree.
Behavior Prediction and Inverse Design for Self-Rotating Skipping Ropes Based on Random Forest and Neural Network
Authors: Qiu Yunlong , Wu Haiyang , Dai Yuntong , Li Kai
Published: March 2024

Self-oscillatory systems have great utility in energy harvesting, engines, and actuators due to their ability to convert ambient energy directly into mechanical work. This characteristic makes their design and implementation highly valuable. Due to the complexity of the motion process and the simultaneous influence of multiple parameters, computing self-oscillatory systems proves to be challenging, especially when conducting inverse parameter design. To simplify the computational process, a combined approach o0f Random Forest (RF) and Backpropagation Neural Network (BPNN) algorithms is employed. The example used is a self-rotating skipping rope made of liquid crystal elastomer (LCE) fiber and a mass block under illumination. Numerically solving the governing equations yields precise solutions for the rotation frequency of the LCE skipping rope under various system parameters. A database containing 138,240 sets of parameter conditions and their corresponding rotation frequencies is constructed to train the RF and BPNN models. The training outcomes indicate that RF and BPNN can accurately predict the self-rotating skipping rope frequency under various parameters, demonstrating high stability and computational efficiency. This approach allows us to discover the influences of distinct parameters on the rotation frequency as well. Moreover, it is capable of inverse design, meaning it can derive the corresponding desired parameter combination from a given rotation frequency. Through this study, a deeper understanding of the dynamic behavior of self-oscillatory systems is achieved, offering a new approach and theoretical foundation for their implementation and construction.
Glucoregulatory Properties of a Protein Hydrolysate from Atlantic Salmon (Salmo salar): Preliminary Characterization and Evaluation of DPP-IV Inhibition and Direct Glucose Uptake In Vitro
Authors: Bjerknes Christian , Wubshet Sileshi Gizachew , Rønning Sissel Beate , Afseth Nils Kristian , Currie Crawford , Framroze Bomi , Hermansen Erland
Published: March 2024

Metabolic disorders are increasingly prevalent conditions that manifest pathophysiologically along a continuum. Among reported metabolic risk factors, elevated fasting serum glucose (FSG) levels have shown the most substantial increase in risk exposure. Ultimately leading to insulin resistance (IR), this condition is associated with notable deteriorations in the prognostic outlook for major diseases, including neurodegenerative diseases, cancer risk, and mortality related to cardiovascular disease. Tackling metabolic dysfunction, with a focus on prevention, is a critically important aspect for human health. In this study, an investigation into the potential antidiabetic properties of a salmon protein hydrolysate (SPH) was conducted, focusing on its potential dipeptidyl peptidase-IV (DPP-IV) inhibition and direct glucose uptake in vitro. Characterization of the SPH utilized a bioassay-guided fractionation approach to identify potent glucoregulatory peptide fractions. Low-molecular-weight (MW) fractions prepared by membrane filtration (MWCO = 3 kDa) showed significant DPP-IV inhibition (IC50 = 1.01 ± 0.12 mg/mL) and glucose uptake in vitro (p ≤ 0.0001 at 1 mg/mL). Further fractionation of the lowest MW fractions (<3 kDa) derived from the permeate resulted in three peptide subfractions. The subfraction with the lowest molecular weight demonstrated the most significant glucose uptake activity (p ≤ 0.0001), maintaining its potency even at a dilution of 1:500 (p ≤ 0.01).
Protein Hydrolysis as a Way to Valorise Squid-Processing Byproducts: Obtaining and Identification of ACE, DPP-IV and PEP Inhibitory Peptides
Authors: Bougatef Hajer , Sila Assaad , Bougatef Ali , Martínez-Alvarez Oscar
Published: March 2024

The industrial processing of Argentine shortfin squid to obtain rings generates a significant amount of protein-rich waste, including the skin, which is rich in collagen and attached myofibrillar proteins. This waste is generally discarded. In this study, skin was used as a source of proteins that were hydrolysed using Trypsin, Esperase® or Alcalase®, which released peptides with antioxidant potential and, in particular, antihypertensive (ACE inhibition), hypoglycemic (DPP-IV inhibition) and/or nootropic (PEP inhibition) potential. Among the three enzymes tested, Esperase® and Alcalase produced hydrolysates with potent ACE-, DPP-IV- and PEP-inhibiting properties. These hydrolysates underwent chromatography fractionation, and the composition of the most bioactive fractions was analysed using HPLC-MS-MS. The fractions with the highest bioactivity exhibited very low IC50 values (16 and 66 µg/mL for ACE inhibition, 97 µg/mL for DPP-IV inhibition and 55 µg/mL for PEP inhibition) and were mainly derived from the hydrolysate obtained using Esperase®. The presence of Leu at the C-terminal appeared to be crucial for the ACE inhibitory activity of these fractions. The DPP-IV inhibitory activity of peptides seemed to be determined by the presence of Pro or Ala in the second position from the N-terminus, and Gly and/or Pro in the last C-terminal positions. Similarly, the presence of Pro in the peptides present in the best PEP inhibitory fraction seemed to be important in the inhibitory effect. These results demonstrate that the skin of the Argentine shortfin squid is a valuable source of bioactive peptides, suitable for incorporation into human nutrition as nutraceuticals and food supplements.
A Comparison of Different Strength Measurement in Taekwondo: Herman Trainer, Manual Tester, and Standing Long Jump
Authors: Boyanmış Ayşe Hazal , Kesilmiş İnci , Akın Manolya , Yilmaz Buse , Uslular Aşina , Karac Ocal Yesim , Andre Hulya
Published: March 2024

Background and Objective: The accurate prediction of strength development relies on identifying the most appropriate measurement methods. This study compared diverse strength measurement techniques to assess their effectiveness in predicting strength development. Participants were taekwondo athletes competing at the red–black belt level or above. Methods: Technical striking forces (palding, dollyeo chagi, dwit chagi, and yeop chagi) were measured using a Herman Digital Trainer fixed to a striking stand. Quadriceps and hamstring strength were assessed with a Lafayette force measuring device. Explosive leg strength was evaluated through a standing long jump test, normalized for leg length. The Pearson correlation coefficient was used to examine relationships between measurement methods. Results: The standing long jump test showed no significant correlation with other strength assessments. A moderate positive correlation was found between Herman digital trainer measurements and Lafayette digital hand-held dynamometer results. A high positive correlation (r = 0.736, p < 0.001) emerged between hamstring strength and palding chagi technical strike force results. Technical strike kicks showed a significant positive correlation with each other and, also, a right foot–left foot correlation was observed. Conclusions: It was concluded that the standing long jump test, which was shown as one of the explosive leg strength measurement methods in field studies as an alternative to laboratory tests, did not correlate with other strength tests; therefore, this test is weak and insufficient to predict strength skills in taekwondo. In addition, this study showed that the hamstring muscle was more predictive in the measurement of technical strength. In future studies, it might be more useful to measure hamstring muscle strength or technical kick strength instead of a standing long jump field test.
Paraneoplastic Syndromes in Hepatocellular Carcinoma, Epidemiology, and Survival: A Retrospective Seven Years Study
Authors: Burciu Calin , Sirli Roxana , Bende Renata , Vuletici Deiana , Miutescu Bogdan , Moga Tudor , Bende Felix , Popescu Alina , Sporea Ioan , Koppandi Oana , Miutescu Eftimie , Iovanescu Dana , Danila Mirela
Published: March 2024

Background and Objectives: Liver cancer poses a significant global health threat, ranking among the top three causes of cancer-related deaths. Patients with hepatocellular carcinoma (HCC) often present with symptoms associated with neoplasms or unusual clinical features such as paraneoplastic syndromes (PNS), including hypoglycemia, hypercholesterolemia, thrombocytosis, and erythrocytosis. Our study aimed to investigate the prevalence, clinical characteristics, and survival outcomes associated with PNS in HCC patients and assess each PNS’s impact on patient survival. Materials and Methods: We conducted a retrospective analysis of PNS clinical features and survival among consecutive HCC patients diagnosed at our department over seven years, comparing them with HCC patients without PNS. The study involved a retrospective data evaluation from 378 patients diagnosed with HCC between January 2016 and October 2023. Results: We obtained a PNS prevalence of 25.7%, with paraneoplastic hypercholesterolemia at 10.9%, hypoglycemia at 6.9%, erythrocytosis at 4.5%, and thrombocytosis at 3.4%. Patients with PNS tended to be younger and predominantly male. Multivariate analysis revealed a strong correlation between PNS and levels of alpha-fetoprotein and tumor size, with diabetes also showing a significant statistical association (p < 0.05). Subgroup analysis based on specific paraneoplastic syndromes demonstrated shorter survival in patients with PNS, albeit without significant statistical differences, except for hypoglycemia (p < 0.0001). Matched analysis indicated a shorter survival rate for patients with PNS, although no significant statistical differences were observed. Conclusions: PNS are frequently observed in HCC cases and are associated with unfavorable prognoses and decreased survival rates due to their correlation with increased tumor burdens. However, they do not independently predict poor survival. The impact of individual PNS on HCC prognosis varies.
Strategies for Enhancing the Stability of Lithium Metal Anodes in Solid-State Electrolytes
Authors: Lee Hanbyeol , Yoon Taeho , Chae Oh
Published: March 2024

The current commercially used anode material, graphite, has a theoretical capacity of only 372 mAh/g, leading to a relatively low energy density. Lithium (Li) metal is a promising candidate as an anode for enhancing energy density; however, challenges related to safety and performance arise due to Li’s dendritic growth, which needs to be addressed. Owing to these critical issues in Li metal batteries, all-solid-state lithium-ion batteries (ASSLIBs) have attracted considerable interest due to their superior energy density and enhanced safety features. Among the key components of ASSLIBs, solid-state electrolytes (SSEs) play a vital role in determining their overall performance. Various types of SSEs, including sulfides, oxides, and polymers, have been extensively investigated for Li metal anodes. Sulfide SSEs have demonstrated high ion conductivity; however, dendrite formation and a limited electrochemical window hinder the commercialization of ASSLIBs due to safety concerns. Conversely, oxide SSEs exhibit a wide electrochemical window, but compatibility issues with Li metal lead to interfacial resistance problems. Polymer SSEs have the advantage of flexibility; however their limited ion conductivity poses challenges for commercialization. This review aims to provide an overview of the distinctive characteristics and inherent challenges associated with each SSE type for Li metal anodes while also proposing potential pathways for future enhancements based on prior research findings.
Fabrication of a Three-Dimensional Microfluidic System from Poly(methyl methacrylate) (PMMA) Using an Intermiscibility Vacuum Bonding Technique
Authors: Li Shu-Cheng , Chiang Chao-Ching , Tsai Yi-Sung , Chen Chien-Jui , Lee Tien-Hsi
Published: March 2024

In this study, the fabrication of microfluidic chips through the bonding of poly (methyl methacrylate) (PMMA) boards featuring designed patterns to create a three-dimensional sandwich structure with embedded microchannels was explored. A key focus was optimization of the interface quality of bonded PMMA pairs by adjusting the solvent, such as such as acetone, alcohol, and their mixture. Annealing was conducted below 50 °C to leverage the advantages of low-temperature bonding. Because of the differences in the chemical reactivity of PMMA toward acetone, alcohol, and their combinations, the resulting defect densities at the bonding interfaces differed significantly under low-temperature annealing conditions. To achieve the optimal sealing integrity, bonding pressures of 30 N, 40 N, and 50 N were evaluated. The interface was analyzed through microstructural examination via optical microscopy and stress measurements were determined using digital photoelasticity, while the bonding strength was assessed through tensile testing.
Micro-Satellite Systems Design, Integration, and Flight
Authors: Naumann Philip , Sands Timothy
Published: March 2024

Within the past decade, the aerospace engineering industry has evolved beyond the constraints of using single, large, custom satellites. Due to the increased reliability and robustness of commercial, off-the-shelf printed circuit board components, missions have instead transitioned towards deploying swarms of smaller satellites. Such an approach significantly decreases the mission cost by reducing custom engineering and deployment expenses. Nanosatellites can be quickly developed with a more modular design at lower risk. The Alpha mission at the Cornell University Space Systems Studio is fabricated in this manner. However, for the purpose of development, the initial proof of concept included a two-satellite system. The manuscript will discuss system engineering approaches used to model and mature the design of the pilot satellite. The two systems that will be primarily focused on are the attitude control system of the carrier nanosatellite and the radio frequency communications on the excreted femto-satellites. Milestones achieved include ChipSat to ChipSat communication, ChipSat to ground station communication, packet creation, error correction, appending a preamble, and filtering the signal. Other achievements include controller traceability/verification and validation, software rigidity tests, hardware endurance testing, Kane damper, and inertial measurement unit tuning. These developments matured the technological readiness level (TRL) of systems in preparation for satellite deployment.
Intelligent Detection and Odor Recognition of Cigarette Packaging Paper Boxes Based on a Homemade Electronic Nose
Authors: Wang Xingguo , Li Hao , Wang Yunlong , Fu Bo , Ai Bin
Published: March 2024

The printing process of box packaging paper can generate volatile organic compounds, resulting in odors that impact product quality and health. An efficient, objective, and cost-effective detection method is urgently needed. We utilized a self-developed electronic nose system to test four different cigarette packaging paper samples. Employing multivariate statistical methods like Principal Component Analysis (PCA), Linear Discriminant Analysis (LDA), Statistical Quality Control (SQC), and Similarity-based Independent Modeling of Class Analogy (SIMCA), we analyzed and processed the collected data. Comprehensive evaluation and quality control models were constructed to assess sample stability and distinguish odors. Results indicate that our electronic nose system rapidly detects odors and effectively performs quality control. By establishing models for quality stability control, we successfully identified samples with acceptable quality and those with odors. To further validate the system’s performance and extend its applications, we collected two types of cigarette packaging paper samples with odor data. Using data augmentation techniques, we expanded the dataset and achieved an accuracy rate of 0.9938 through classification and discrimination. This highlights the significant potential of our self-developed electronic nose system in recognizing cigarette packaging paper odors and odorous samples.
Characterization of Probiotic Properties and Whole-Genome Analysis of Lactobacillus johnsonii N5 and N7 Isolated from Swine
Authors: Wang Kun , Wang Yu , Gu Lifang , Yu Jinyan , Liu Qianwen , Zhang Ruiqi , Liang Guixin , Chen Huan , Gu Fang , Liu Haoyu , Jiao Xin’an , Zhang Yunzeng
Published: March 2024

In our previous microbiome profiling analysis, Lactobacillus (L.) johnsonii was suggested to contribute to resistance against chronic heat stress-induced diarrhea in weaned piglets. Forty-nine L. johnsonii strains were isolated from these heat stress-resistant piglets, and their probiotic properties were assessed. Strains N5 and N7 exhibited a high survival rate in acidic and bile environments, along with an antagonistic effect against Salmonella. To identify genes potentially involved in these observed probiotic properties, the complete genome sequences of N5 and N7 were determined using a combination of Illumina and nanopore sequencing. The genomes of strains N5 and N7 were found to be highly conserved, with two N5-specific and four N7-specific genes identified. Multiple genes involved in gastrointestinal environment adaptation and probiotic properties, including acidic and bile stress tolerance, anti-inflammation, CAZymes, and utilization and biosynthesis of carbohydrate compounds, were identified in both genomes. Comparative genome analysis of the two genomes and 17 available complete L. johnsonii genomes revealed 101 genes specifically harbored by strains N5 and N7, several of which were implicated in potential probiotic properties. Overall, this study provides novel insights into the genetic basis of niche adaptation and probiotic properties, as well as the genome diversity of L. johnsonii.
Risk Factors Associated with Toxoplasma gondii in Patients with Cardiovascular Diseases from Western Romania
Authors: Dragomir Angela , Lupu Maria Alina , Maciuceanu Cosmin Gheorghe , Chicea Liana Maria , Olariu Tudor Rares
Published: March 2024

Background: Limited data are available in the existing literature regarding the seroepidemiology of T. gondii infection among cardiovascular patients. We aimed to comprehensively assess the prevalence of T. gondii infection and associated risk factors among Romanian cardiovascular patients. Methods: Serologic testing was conducted in 1205 patients with cardiovascular diseases to demonstrate the presence of T. gondii antibodies. An avidity test was performed in patients with detectable IgG and IgM antibodies. A structured questionnaire was designed to identify the potential risk factors associated with T. gondii. Results: The overall seroprevalence of T. gondii antibodies was 52.1%, with the highest value observed in patients diagnosed with dilated cardiomyopathy (66.66%) and the lowest in patients with myopericarditis (30.0%). The 11 patients found with detectable IgM and IgG antibodies had a high avidity test result. A patient’s area of residence, gender, educational level, owning dogs, owning any pet, and toxoplasmosis awareness were significantly associated with T. gondii seropositivity in multiple logistic regression analyses. Conclusions: This study provides novel and valuable insights into the seroprevalence and risk factors associated with T. gondii among Romanian cardiovascular patients. Our findings reiterate the importance of toxoplasmosis awareness and health education for better control and prevention of infection with T. gondii.
The Presence of a Virulent Clone of Leptospira interrogans Serovar Canicola in Confirmed Cases of Asymptomatic Dog Carriers in Mexico
Authors: Carmona Gasca Carlos Alfredo , Martínez González Sergio , Castillo Sánchez Luz Olivia , Rodríguez Reyes Ernesto Armando , Cárdenas Marrufo María Fidelia , Vado Solís Ignacio , Castañeda Miranda Giselle , López Huitrado Lilia Patricia , de la Peña-Moctezuma Alejandro
Published: March 2024

Leptospirosis is a neglected zoonotic disease that commonly affects cattle, pigs, horses, and dogs in many countries. Infection in dogs is usually subclinical, but acute cases of leptospirosis may occur along with systemic failure, which may become fatal. After recovery from an acute infection, dogs may become asymptomatic carriers and shed pathogenic leptospires through urine for long periods of time. Here, a study of ten different cases of leptospirosis is presented, showing the relevance of dogs as asymptomatic carriers of pathogenic Leptospira. The diagnosis was confirmed via isolation and further serological and genetic identification. Four Leptospira isolates (LOCaS28, 31, 34, and 46) were obtained from the kidneys and urine samples of 58 dogs destined for destruction (6.89%) at a Canine Control Center in Mexico City. No spirochetes were observed in the urine samples of those Leptospira-positive dogs examined under dark-field microscopy, and no clinical signs of disease were observed either. Six additional isolates were obtained: two came from asymptomatic carrier dogs (CEL60 and UADY22); another isolate came from an asymptomatic dog that was a pack companion of a clinically ill dog with fatal leptospirosis (AGFA24); and finally, three isolates were taken from dogs that died of leptospirosis (LOCaS59, Citlalli, and Nayar1). Nine out of the ten isolates were identified as being from the serogroup Canicola via cross-absorption MAT using reference strains and specific antisera, and their identity was genetically confirmed as Canicola ST34 via multi-locus sequencing typing (MLST). In contrast, the isolate Nayar1 was identified as serovar Copenhageni ST2. Interestingly, the asymptomatic dogs from which Leptospira isolates were recovered consistently showed high antibody titers in the microscopic agglutination test (MAT), revealing values of at least 1:3200 against serogroup Canicola and lower titer values against other serogroups. Isolates showed different virulence levels in the hamster model. Taken as a whole, all these findings confirmed that dogs may act as asymptomatic carriers of pathogenic leptospires and possibly spread them out to the environment, thus representing an active public health risk. The results also showed that the Canicola ST34 clone is the most prevalent Leptospira serovar in dogs in Mexico, and finally that the old-fashioned MAT is a good alternative for the detection of presumptive Leptospira asymptomatic carrier dogs.