bluuebunny/crossref_metadata_2025 huggingface dataset:
Nimodipine Treatment Protects Auditory Hair Cells from Cisplatin-Induced Cell Death Accompanied by Upregulation of LMO4
Authors: Fritzsche Saskia , Strauss Christian , Scheller Christian , Leisz Sandra
Published: May 2022

Ototoxicity is one of the main dose-limiting side effects of cisplatin chemotherapy and impairs the quality of life of tumor patients dramatically. Since there is currently no established standard therapy targeting hearing loss in cisplatin treatment, the aim of this study was to investigate the effect of nimodipine and its role in cell survival in cisplatin-associated hearing cell damage. To determine the cytotoxic effect, the cell death rate was measured using undifferentiated and differentiated UB/OC−1 and UB/OC−2 cells, after nimodipine pre-treatment and stress induction by cisplatin. Furthermore, immunoblot analysis and intracellular calcium measurement were performed to investigate anti-apoptotic signaling, which was associated with a reduced cytotoxic effect after nimodipine pre-treatment. Cisplatin’s cytotoxic effect was significantly attenuated by nimodipine up to 61%. In addition, nimodipine pre-treatment counteracted the reduction in LIM Domain Only 4 (LMO4) by cisplatin, which was associated with increased activation of Ak strain transforming/protein kinase B (Akt), cAMP response element-binding protein (CREB), and signal transducers and activators of transcription 3 (Stat3). Thus, nimodipine presents a potentially well-tolerated substance against the ototoxicity of cisplatin, which could result in a significant improvement in patients’ quality of life.
Mutation of Protoporphyrinogen IX Oxidase Gene Causes Spotted and Rolled Leaf and Its Overexpression Generates Herbicide Resistance in Rice
Authors: Liu Xin , Deng Xiao-Jian , Li Chun-Yan , Xiao Yong-Kang , Zhao Ke , Guo Jia , Yang Xiao-Rong , Zhang Hong-Shan , Chen Cong-Ping , Luo Ya-Ting , Tang Yu-Lin , Yang Bin , Sun Chang-Hui , Wang Ping-Rong
Published: May 2022

Protoporphyrinogen IX (Protogen IX) oxidase (PPO) catalyzes the oxidation of Protogen IX to Proto IX. PPO is also the target site for diphenyl ether-type herbicides. In plants, there are two PPO encoding genes, PPO1 and PPO2. To date, no PPO gene or mutant has been characterized in monocotyledonous plants. In this study, we isolated a spotted and rolled leaf (sprl1) mutant in rice (Oryza sativa). The spotted leaf phenotype was sensitive to high light intensity and low temperature, but the rolled leaf phenotype was insensitive. We confirmed that the sprl1 phenotypes were caused by a single nucleotide substitution in the OsPPO1 (LOC_Os01g18320) gene. This gene is constitutively expressed, and its encoded product is localized to the chloroplast. The sprl1 mutant accumulated excess Proto(gen) IX and reactive oxygen species (ROS), resulting in necrotic lesions. The expressions of 26 genes associated with tetrapyrrole biosynthesis, photosynthesis, ROS accumulation, and rolled leaf were significantly altered in sprl1, demonstrating that these expression changes were coincident with the mutant phenotypes. Importantly, OsPPO1-overexpression transgenic plants were resistant to the herbicides oxyfluorfen and acifluorfen under field conditions, while having no distinct influence on plant growth and grain yield. These finding indicate that the OsPPO1 gene has the potential to engineer herbicide resistance in rice.
High Diffusion Permeability of Anion-Exchange Membranes for Ammonium Chloride: Experiment and Modeling
Authors: Skolotneva Ekaterina , Tsygurina Kseniia , Mareev Semyon , Melnikova Ekaterina , Pismenskaya Natalia , Nikonenko Victor
Published: May 2022

It is known that ammonium has a higher permeability through anion exchange and bipolar membranes compared to K+ cation that has the same mobility in water. However, the mechanism of this high permeability is not clear enough. In this study, we develop a mathematical model based on the Nernst–Planck and Poisson’s equations for the diffusion of ammonium chloride through an anion-exchange membrane; proton-exchange reactions between ammonium, water and ammonia are taken into account. It is assumed that ammonium, chloride and OH− ions can only pass through membrane hydrophilic pores, while ammonia can also dissolve in membrane matrix fragments not containing water and diffuse through these fragments. It is found that due to the Donnan exclusion of H+ ions as coions, the pH in the membrane internal solution increases when approaching the membrane side facing distilled water. Consequently, there is a change in the principal nitrogen-atom carrier in the membrane: in the part close to the side facing the feed NH4Cl solution (pH < 8.8), it is the NH4+ cation, and in the part close to distilled water, NH3 molecules. The concentration of NH4+ reaches almost zero at a point close to the middle of the membrane cross-section, which approximately halves the effective thickness of the diffusion layer for the transport of this ion. When NH3 takes over the nitrogen transport, it only needs to pass through the other half of the membrane. Leaving the membrane, it captures an H+ ion from water, and the released OH− moves towards the membrane side facing the feed solution to meet the NH4+ ions. The comparison of the simulation with experiment shows a satisfactory agreement.
Metabolic Profiles Reveal Changes in the Leaves and Roots of Rapeseed (Brassica napus L.) Seedlings under Nitrogen Deficiency
Authors: Shen Xinjie , Yang Ling , Han Peipei , Gu Chiming , Li Yinshui , Liao Xing , Qin Lu
Published: May 2022

Rapeseed (Brassica napus L.) is an important oil crop species and plays a crucial role in supplying edible oil worldwide. However, rapeseed production in the field is often severely inhibited due to nitrogen (N) deficiency. Metabolites play key roles in plant growth and resistance to environmental stress, but little is known about the differential synthesis and accumulation of metabolites underlying rapeseed adaptation to N deficiency. Here, we studied the phenotypic response and used LC–electrospray ionization (ESI), ESI–MS/MS, and widely untargeted metabolomic approaches to detect differences in rapeseed under normal N (HN) and N-deficient (LN) conditions. The results showed that N deficiency severely inhibited rapeseed shoot growth and promoted rapeseed root architectural changes under LN conditions. In total, 574 metabolites were detected, and there were 175 and 166 differentially accumulated metabolites in the leaves and roots between the HN and LN conditions, respectively. The significantly differentially accumulated metabolites were involved in four primary metabolic pathways, namely, sucrose, phenylalanine, amino acid, and tricarboxylic acid cycle metabolism. Notably, we found that plant hormones have distinct accumulation patterns in rapeseed and coordinate to play crucial roles in both maintaining growth and protecting against damage from plant disease under HN and LN conditions. Moreover, our results indicated that flavonoid compounds, especially anthocyanins and rutin, may play important roles in increasing root cell resistance to oxidative damage and soil pathogen infections. Overall, this work provides valuable information for understanding the overall metabolite changes in rapeseed under N deficiency conditions, which may be beneficial for improving and producing new varieties of rapeseed capable of high yields under low N conditions.
SOX2 Is a Univocal Marker for Human Oral Mucosa Epithelium Useful in Post-COMET Patient Characterization
Authors: Attico Eustachio , Galaverni Giulia , Bianchi Elisa , Losi Lorena , Manfredini Rossella , Lambiase Alessandro , Rama Paolo , Pellegrini Graziella
Published: May 2022

Total bilateral Limbal Stem Cells Deficiency is a pathologic condition of the ocular surface due to loss or impairment of corneal stem cell function, altering homeostasis of the corneal epithelium. Cultivated Oral Mucosa Epithelial Transplantation (COMET) is the only autologous treatment for this pathology. During the follow-up, a proper characterization of the transplanted oral mucosa on the ocular surface supports understanding the regenerative process. The previously proposed markers for oral mucosa identification (e.g., keratins 3 and 13) are co-expressed by corneal and conjunctival epithelia. Here, we propose a new specific marker to distinguish human oral mucosa from the epithelia of the ocular surface. We compared the transcriptome of holoclones (stem cells) from the human oral mucosa, limbal and conjunctival cultures by microarray assay. High expression of SOX2 identified the oral mucosa vs. cornea and conjunctiva, while PAX6 was highly expressed in corneal and conjunctival epithelia. The transcripts were validated by qPCR, and immunological methods identified the related proteins. Finally, the proposed markers were used to analyze a 10-year follow-up aniridic patient treated by COMET. These findings will support the follow-up analysis of COMET treated patients and help to shed light on the mechanism of corneal repair and regeneration.
Making Connections: Mesenchymal Stem Cells Manifold Ways to Interact with Neurons
Authors: Tarasiuk Olga , Ballarini Elisa , Donzelli Elisabetta , Rodriguez-Menendez Virginia , Bossi Mario , Cavaletti Guido , Scuteri Arianna
Published: May 2022

Mesenchymal Stem Cells (MSCs) are adult multipotent cells able to increase sensory neuron survival: direct co-culture of MSCs with neurons is pivotal to observe a neuronal survival increase. Despite the identification of some mechanisms of action, little is known about how MSCs physically interact with neurons. The aim of this paper was to investigate and characterize the main mechanisms of interaction between MSCs and neurons. Morphological analysis showed the presence of gap junctions and tunneling nanotubes between MSCs and neurons only in direct co-cultures. Using a diffusible dye, we observed a flow from MSCs to neurons and further analysis demonstrated that MSCs donated mitochondria to neurons. Treatment of co-cultures with the gap junction blocker Carbenoxolone decreased neuronal survival, thus demonstrating the importance of gap junctions and, more in general, of cell communication for the MSC positive effect. We also investigated the role of extracellular vesicles; administration of direct co-cultures-derived vesicles was able to increase neuronal survival. In conclusion, our study demonstrates the presence and the importance of multiple routes of communication between MSCs and neurons. Such knowledge will allow a better understanding of the potential of MSCs and how to maximize their positive effect, with the final aim to provide the best protective treatment.
APOE Molecular Spectrum in a French Cohort with Primary Dyslipidemia
Authors: Abou Khalil Yara , Marmontel Oriane , Ferrières Jean , Paillard François , Yelnik Cécile , Carreau Valérie , Charrière Sybil , Bruckert Eric , Gallo Antonio , Giral Philippe , Philippi Anne , Bluteau Olivier , Boileau Catherine , Abifadel Marianne , Di-Filippo Mathilde , Carrié Alain , Rabès Jean-Pierre , Varret Mathilde
Published: May 2022

Primary hypercholesterolemia is characterized by elevated LDL-cholesterol (LDL-C) levels isolated in autosomal dominant hypercholesterolemia (ADH) or associated with elevated triglyceride levels in familial combined hyperlipidemia (FCHL). Rare APOE variants are known in ADH and FCHL. We explored the APOE molecular spectrum in a French ADH/FCHL cohort of 5743 unrelated probands. The sequencing of LDLR, PCSK9, APOB, and APOE revealed 76 carriers of a rare APOE variant, with no mutation in LDLR, PCSK9, or APOB. Among the 31 APOE variants identified here, 15 are described in ADH, 10 in FCHL, and 6 in both probands. Five were previously reported with dyslipidemia and 26 are novel, including 12 missense, 5 synonymous, 2 intronic, and 7 variants in regulatory regions. Sixteen variants were predicted as pathogenic or likely pathogenic, and their carriers had significantly lower polygenic risk scores (wPRS) than carriers of predicted benign variants. We observed no correlation between LDL-C levels and wPRS, suggesting a major effect of APOE variants. Carriers of p.Leu167del were associated with a severe phenotype. The analysis of 11 probands suggests that carriers of an APOE variant respond better to statins than carriers of a LDLR mutation. Altogether, we show that the APOE variants account for a significant contribution to ADH and FCHL.
The Development of Novel Drug Treatments for Stroke Patients: A Review
Authors: Frank Dmitry , Zlotnik Alexander , Boyko Matthew , Gruenbaum Benjamin Fredrick
Published: May 2022

Acute ischemic stroke is a critical condition that can result in disability and death. The consequences of this medical condition depend on various factors, including the size of the stroke, affected brain region, treatment onset, and the type of treatment. The primary objective of stroke treatment is to restart ischemic penumbra tissue perfusion and reduce infarct volume by sustaining blood flow. Recent research on the condition’s pathological pathways and processes has significantly improved treatment options beyond restoring perfusion. Many studies have concentrated on limiting injury severity via the manipulation of molecular mechanisms of ischemia, particularly in animal research. This article reviews completed and ongoing research on the development of acute ischemic stroke drugs. This study focuses on three main categories of antithrombotic drugs, thrombolytic drugs, and neuroprotective agents. The paper outlines findings from animal and clinical trials and explores the working mechanisms of these drugs.
A Tetranuclear Dysprosium Schiff Base Complex Showing Slow Relaxation of Magnetization
Authors: Gebrezgiabher Mamo , Schlittenhardt Sören , Rajnák Cyril , Sergawie Assefa , Ruben Mario , Thomas Madhu , Boča Roman
Published: May 2022

A tetranuclear dysprosium Schiff base complex was isolated by reacting dysprosium chloride with 2-hydroxy-3-methoxybenzaldehyde and 2-(aminomethyl)pyridine in-situ under basic conditions. The isolated Dy(III) complex was characterized by elemental analyses, single crystal X-ray diffraction and molecular spectroscopy. The complex crystallizes in the triclinic space group P-1 with unit cell parameters of a = 10.2003 (4), b = 13.8602 (5), c = 14.9542 (6), α = 94.523 (3), β = 109.362 (4), and γ = 99.861 (3). The magnetic properties of 1 have been investigated by DC and AC susceptibility measurements. The DC measurements reveal weak exchange coupling of antiferromagnetic nature. In the AC measurement, the complex shows a slow relaxation of magnetization in the absence of an external magnetic field.
What Is Significant in Modern Augmented Reality: A Systematic Analysis of Existing Reviews
Authors: Nikolaidis Athanasios
Published: May 2022

Augmented reality (AR) is a field of technology that has evolved drastically during the last decades, due to its vast range of applications in everyday life. The aim of this paper is to provide researchers with an overview of what has been surveyed since 2010 in terms of AR application areas as well as in terms of its technical aspects, and to discuss the extent to which both application areas and technical aspects have been covered, as well as to examine whether one can extract useful evidence of what aspects have not been covered adequately and whether it is possible to define common taxonomy criteria for performing AR reviews in the future. To this end, a search with inclusion and exclusion criteria has been performed in the Scopus database, producing a representative set of 47 reviews, covering the years from 2010 onwards. A proper taxonomy of the results is introduced, and the findings reveal, among others, the lack of AR application reviews covering all suggested criteria.
Evaluation Simulator Platform for Extended Collision Risk of Autonomous Surface Vehicles
Authors: Vagale Anete
Published: May 2022

Autonomous surface vehicles need to be at least as safe as conventional vessels, if not safer, when navigating on waters. With a great deal of navigation algorithms for surface vessels out there, the safety of their produced paths is questionable, and, in most cases, complicated to assess and compare. Hence, this paper proposes a method for extended collision risk assessment for paths generated by autonomous navigation algorithms as follows: (1) static, dynamic, and historic risk factors are calculated; (2) individual risk value is determined using a fuzzy inference system; (3) the extended collision risk assessment (ECRA) score is acquired using a root-mean-square method. Finally, a comparison of the ECRA score of each path determines the path with the lowest risk. The validation results show that the proposed method is able to detect lower/higher risk scenarios and assign an adequate risk value in most cases. Risk reduction for cautious paths varies up to 8.43%, while risk increases for incautious paths—up to 57.98%. The results indicate that the method could be used for navigation algorithm evaluation and comparison with some improvements. This research also reveals several promising future directions and applications of the method.
Oxygen-Plasma-Induced Hetero-Interface NiFe2O4/NiMoO4 Catalyst for Enhanced Electrochemical Oxygen Evolution
Authors: Xu Nuo , Peng Wei , Lv Lei , Xu Peng , Wang Chenxu , Li Jiantao , Luo Wen , Zhou Liang
Published: May 2022

The electrolysis of water to produce hydrogen is an effective method for solving the rapid consumption of fossil fuel resources and the problem of global warming. The key to its success is to design an oxygen evolution reaction (OER) electrocatalyst with efficient conversion and reliable stability. Interface engineering is one of the most effective approaches for adjusting local electronic configurations. Adding other metal elements is also an effective way to enrich active sites and improve catalytic activity. Herein, high-valence iron in a heterogeneous interface of NiFe2O4/NiMoO4 composite was obtained through oxygen plasma to achieve excellent electrocatalytic activity and stability. In particular, 270 mV of overpotential is required to reach a current density of 50 mA cm−2, and the overpotential required to reach 500 mA cm−2 is only 309 mV. The electron transfer effect for high-valence iron was determined by X-ray photoelectron spectroscopy (XPS). The fast and irreversible reconstruction and the true active species in the catalytic process were identified by in situ Raman, ex situ XPS, and ex situ transmission electron microscopy (TEM) measurements. This work provides a feasible design guideline to modify electronic structures, promote a metal to an active oxidation state, and thus develop an electrocatalyst with enhanced OER performance.
Development of a Kinetic Model for the Redox Reactions of Co2.4Ni0.6O4 and SiO2/Co2.4Ni0.6O4 Oxides for Thermochemical Energy Storage
Authors: Portilla-Nieto Yasmina , Bielsa Daniel , Dauvergne Jean-Luc , Hernaiz Marta , Aranzabe Estibaliz , Doppiu Stefania , Palomo del Barrio Elena
Published: May 2022

One of the possible solutions for the transition of the actual energetic model is the use of thermal energy storage technologies. Among them, thermochemical energy storage based on redox reactions involving metal oxides is very promising due to its high energy density. This paper deals with the development of the kinetic study based on data extracted from the thermogravimetric analysis of a cobalt-nickel mixed oxide (Co2.4Ni0.6O4) without and with the addition of SiO2 particles to improve the cyclability. The results show that in the reduction reaction the activation energy is not affected by the addition of SiO2 particles while in the oxidation reaction an increase in the activation energy is observed. The theoretical models fitting with the experimental data are different for each material in the reduction reaction. The mixed oxide is controlled by a nucleation and growth mechanism for conversion ratios higher than 0.5, while the added material is controlled by diffusion mechanisms. In the oxidation reaction, the two materials are controlled by a nucleation and growth mechanism for conversion ratios higher than 0.5.
A Review of Non-Destructive Testing (NDT) Techniques for Defect Detection: Application to Fusion Welding and Future Wire Arc Additive Manufacturing Processes
Authors: Shaloo Masoud , Schnall Martin , Klein Thomas , Huber Norbert , Reitinger Bernhard
Published: May 2022

In Wire and Arc Additive Manufacturing (WAAM) and fusion welding, various defects such as porosity, cracks, deformation and lack of fusion can occur during the fabrication process. These have a strong impact on the mechanical properties and can also lead to failure of the manufactured parts during service. These defects can be recognized using non-destructive testing (NDT) methods so that the examined workpiece is not harmed. This paper provides a comprehensive overview of various NDT techniques for WAAM and fusion welding, including laser-ultrasonic, acoustic emission with an airborne optical microphone, optical emission spectroscopy, laser-induced breakdown spectroscopy, laser opto-ultrasonic dual detection, thermography and also in-process defect detection via weld current monitoring with an oscilloscope. In addition, the novel research conducted, its operating principle and the equipment required to perform these techniques are presented. The minimum defect size that can be identified via NDT methods has been obtained from previous academic research or from tests carried out by companies. The use of these techniques in WAAM and fusion welding applications makes it possible to detect defects and to take a step towards the production of high-quality final components.
Tribological Performance of High-Entropy Coatings (HECs): A Review
Authors: Patel Payank , Roy Amit , Sharifi Navid , Stoyanov Pantcho , Chromik Richard R. , Moreau Christian
Published: May 2022

Surface coatings that operate effectively at elevated temperatures provide compatibility with critical service conditions as well as improved tribological performance of the components. High-entropy coatings (HECs), including metallic, ceramics, and composites, have gained attention all over the world and developed rapidly over the past 18 years, due to their excellent mechanical and tribological properties. High-entropy alloys (HEAs) are defined as alloys containing five or more principal elements in equal or close to equal atomic percentage. Owing to the high configurational entropy compared to conventional alloys, HEAs are usually composed of a simple solid solution phase, such as the BCC and FCC phases, instead of complex, brittle intermetallic phases. Several researchers have investigated the mechanical, oxidation, corrosion and wear properties of high-entropy oxides, carbides, borides, and silicates using various coating and testing techniques. More recently, the friction and wear characteristics of high-entropy coatings (HECs) have gained interest within various industrial sectors, mainly due to their favourable mechanical and tribological properties at high temperatures. In this review article, the authors identified the research studies and developments in high-entropy coatings (HECs) fabricated on various substrate materials using different synthesis methods. In addition, the current understanding of the HECs characteristics is critically reviewed, including the fabrication routes of targets/feedstock, synthesis methods utilized in various research studies, microstructural and tribological behaviour from room temperature to high temperatures.
Topology Identification of Time-Scales Complex Networks
Authors: Pei Yong , Chen Churong , Pi Dechang
Published: May 2022

This paper studies a topology identification problem of complex networks with dynamics on different time scales. Using the adaptive synchronization method, some criteria for a successful estimation are obtained. In particular, by regulating the original network to synchronize with an auxiliary chaotic network, this work further explores a way to avoid the precondition of linear independence. When the adaptive controller fails to achieve the outer synchronization, an impulsive control method is used. In the end, we conclude with three numerical simulations. The results obtained in this paper generalize continuous, discrete with arbitrary time step size and mixed cases.
One- and Two-Dimensional Analytical Solutions of Thermal Stress for Bimodular Functionally Graded Beams under Arbitrary Temperature Rise Modes
Authors: Xue Xuan-Yi , Wen Si-Rui , Sun Jun-Yi , He Xiao-Ting
Published: May 2022

In this study, we analytically solved the thermal stress problem of a bimodular functionally graded bending beam under arbitrary temperature rise modes. First, based on the strain suppression method in a one-dimensional case, we obtained the thermal stress of a bimodular functionally graded beam subjected to bending moment under arbitrary temperature rise modes. Using the stress function method based on compatibility conditions, we also derived two-dimensional thermoelasticity solutions for the same problem under pure bending and lateral-force bending, respectively. During the solving, the number of unknown integration constants is doubled due to the introduction of bimodular effect; thus, the determination for these constants depends not only on the boundary conditions, but also on the continuity conditions at the neutral layer. The comparisons indicate that the one- and two-dimensional thermal stress solutions are consistent in essence, with a slight difference in the axial stress, which exactly reflects the distinctions of one- and two-dimensional problems. In addition, the temperature rise modes in this study are not explicitly indicated, which further expands the applicability of the solutions obtained. The originality of this work is that the one- and two-dimensional thermal stress solutions for bimodular functionally graded beams are derived for the first time. The results obtained in this study may serve as a theoretical reference for the analysis and design of beam-like structures with obvious bimodular functionally graded properties in a thermal environment.
Exact Solvability Conditions for the Non-Local Initial Value Problem for Systems of Linear Fractional Functional Differential Equations
Authors: Dilna Natalia , Fečkan Michal
Published: May 2022

The exact conditions sufficient for the unique solvability of the initial value problem for a system of linear fractional functional differential equations determined by isotone operators are established. In a sense, the conditions obtained are optimal. The method of the test elements intended for the estimation of the spectral radius of a linear operator is used. The unique solution is presented by the Neumann’s series. All theoretical investigations are shown in the examples. A pantograph-type model from electrodynamics is studied.
A Fuzzy Simheuristic for the Permutation Flow Shop Problem under Stochastic and Fuzzy Uncertainty
Authors: Castaneda Juliana , Martin Xabier , Ammouriova Majsa , Panadero Javier , Juan Angel
Published: May 2022

Stochastic, as well as fuzzy uncertainty, can be found in most real-world systems. Considering both types of uncertainties simultaneously makes optimization problems incredibly challenging. In this paper, we analyze the permutation flow shop problem (PFSP) with both stochastic and fuzzy processing times. The main goal is to find the solution (permutation of jobs) that minimizes the expected makespan. However, due to the existence of uncertainty, other characteristics of the solution are also taken into account. In particular, we illustrate how survival analysis can be employed to enrich the probabilistic information given to decision-makers. To solve the aforementioned optimization problem, we extend the concept of a simheuristic framework so it can also include fuzzy elements. Hence, both stochastic and fuzzy uncertainty are simultaneously incorporated in the PFSP. In order to test our approach, classical PFSP instances have been adapted and extended, so that processing times become either stochastic or fuzzy. The experimental results show the effectiveness of the proposed approach when compared with more traditional ones.
Simulation and State Feedback Control of a Pressure Swing Adsorption Process to Produce Hydrogen
Authors: Martínez García Mario , Rumbo Morales Jesse Y. , Torres Gerardo Ortiz , Rodríguez Paredes Salvador A. , Vázquez Reyes Sebastián , Sorcia Vázquez Felipe de J. , Pérez Vidal Alan F. , Valdez Martínez Jorge S. , Pérez Zúñiga Ricardo , Renteria Vargas Erasmo M.
Published: May 2022

One of the separation processes used for the production and purification of hydrogen is molecular sieve adsorption using the Pressure Swing Adsorption (PSA) method. The process uses two beds containing activated carbon and a sequence of four steps (adsorption, depressurization, purge, and repressurization) for hydrogen production and purification. The initial composition is 0.11 CO, 0.61 H2, and 0.28 CH4 in molar fractions. The aim of this work is to bring the purity of hydrogen to 0.99 in molar fraction and implement controllers that can maintain the desired purity even in the presence of the disturbances that occur in the PSA process. The controller design (discrete PID and state feedback control) was based on the Hammerstein–Wiener model, which had an 80% fit over the rigorous PSA model. Both controllers were validated on a virtual plant of the PSA process, showing great performance and robustness against disturbances. The results obtained show that it is possible to follow the desired trajectory and attenuate double disturbances, while managing to maintain the purity of hydrogen at a value of 0.99 in molar fraction, which meets the international standards to be used as a biofuel.
Intent-Controllable Citation Text Generation
Authors: Jung Shing-Yun , Lin Ting-Han , Liao Chia-Hung , Yuan Shyan-Ming , Sun Chuen-Tsai
Published: May 2022

We study the problem of controllable citation text generation by introducing a new concept to generate citation texts. Citation text generation, as an assistive writing approach, has drawn a number of researchers’ attention. However, current research related to citation text generation rarely addresses how to generate the citation texts that satisfy the specified citation intents by the paper’s authors, especially at the beginning of paper writing. We propose a controllable citation text generation model that extends a pre-trained sequence to sequence models, namely, BART and T5, by using the citation intent as the control code to generate the citation text, meeting the paper authors’ citation intent. Experimental results demonstrate that our model can generate citation texts semantically similar to the reference citation texts and satisfy the given citation intent. Additionally, the results from human evaluation also indicate that incorporating the citation intent may enable the models to generate relevant citation texts almost as scientific paper authors do, even when only a little information from the citing paper is available.
Analysis of Industrial Engineering Students’ Perception after a Multiple Integrals-Based Activity with a Fourth-Year Student
Authors: Giménez Anuar R. , Martín-Vaquero Jesús , Rodríguez-Martín Manuel
Published: May 2022

In industrial engineering degrees in Spain, mathematics subjects are usually taught during the first two academic years. Consequently, it is often the case that students sometimes do not feel motivated to learn subjects such as Mathematics II (calculus). Nevertheless, this subject is fundamental for understanding other subjects in the degree study plan, as well as for the graduate’s future professional career as an engineer. To address this, a problem-based teaching methodology was carried out with the help of a fourth-year student who explained an activity to first-year students in a manner which was both friendly and approachable. In this experiment, the student went through a series of practical problems taken from different engineering subjects, which required multivariable integrals to be calculated and which he had learned in mathematics as a first-year student. In addition, a method based on pre-test and post-test assessments was applied. From this work, various benefits were observed in terms of learning, as well as an increase in the level of motivation of first-year students. There was a greater appreciation of the usefulness of calculus and computer programs to solve real-life problems, and the students generally responded positively to this type of activity.
A General, Label-Free and Homogeneous Electrochemical Strategy for Probing of Protease Activity and Screening of Inhibitor
Authors: Feng Yunxiao , Liu Gang , Zhang Fan , Liu Jianwen , La Ming , Xia Ning
Published: May 2022

Proteases play a critical role in regulating various physiological processes from protein digestion to wound healing. Monitoring the activity of proteases and screening their inhibitors as potential drug molecules are of great importance for the early diagnosis and treatment of many diseases. In this work, we reported a general, label-free and homogeneous electrochemical method for monitoring protease activity based on the peptide–copper interaction. Cleavage of peptide substrate results in the generation of a copper-binding chelator peptide with a histidine residue in the first or third position (His1 or His3) at the N-terminal. The redox potential and current of copper coordinated with the product are different from the free copper or the copper complex with the substrate, thus allowing for the detection of protease activity. Angiotensin-converting enzyme (ACE) and thrombin were determined as the model analytes. The label-free and homogeneous electrochemical method can be used for screening protease inhibitors with high simplicity and sensitivity.
An Assessment of the Lactic Acid-Producing Potential of Bacterial Strains Isolated from Food Waste
Authors: Uwamahoro Henriette Peace , Li Fuyu , Timilsina Arbindra , Liu Binbin , Wang Xinzhen , Tian Yinping
Published: May 2022

Lactic acid (LA) is widely used in many industries as a crucial starting material in food products, bio-based materials, and biodegradable polymers. The goals of this research were to isolate LA bacteria from food wastes, assess their potential for LA production, and study their growth characteristics. In this study, six bacterial strains were isolated from food waste and identified using 16S rRNA gene sequencing; namely, Weissella viridescens WJ39, Leuconostoc lactis YS33, Leuconostoc citreum KD42, Leuconostoc mesenteroides VN60, Macrococcus caseolyticus FCI29, and Weissella confusa RG41. W. viridescens WJ39 showed the highest potential for lactic acid production (17.56 g L−1day−1), and the lowest potential was found in L. lactis YS33 (14.09 g L−1day−1). There were significant differences (p < 0.05) in the LA production rates among Weissella spp., Leuconostoc spp., and Macrococcus spp. Moreover, dramatic differences in growth rate were observed among the six strains. W. viridescens WJ39 exhibited the highest growth rate (0.80 h−1), while M. caseolyticus FCI29 exhibited the lowest growth rate (0.57 h−1). W. viridescens WJ39 also exhibited lactic acid production (at a rate around 2 g L−1day−1) in a lab incubation experiment with food waste as a nutrient source. The draft genome of W. viridescens WJ39 with 16 contigs was constructed with an N50 of 215217 bp. The genome size was approximately 1.54 Mb, with a GC content of 41%. A hicD gene, known to catalyze the conversion of pyruvate to D-lactate, was discovered in the genome. This study illustrated the potential for the production of lactic acid from food waste with lactic acid bacteria.
Functional Foods, Nutraceuticals and Probiotics: A Focus on Human Health
Authors: Damián Morayma Ramírez , Cortes-Perez Naima G. , Quintana Erika T. , Ortiz-Moreno Alicia , Garfias Noguez Cynthia , Cruceño-Casarrubias Carlos Eugenio , Sánchez Pardo María Elena , Bermúdez-Humarán Luis G.
Published: May 2022

Functional foods are classified as traditional or staple foods that provide an essential nutritional level and share potentially positive effects on host health, including the reduction of disease by optimizing the immune system’s ability to prevent and control infections by pathogens, as well as pathologies that cause functional alterations in the host. This chapter reviews the most recent research and advances in this area and discusses some perspectives on what the future holds in this area.
Boron Impurity Deposition on a Si(100) Surface in a SiHCl3-BCl3-H2 System for Electronic-Grade Polysilicon Production
Authors: Yang Qinghong , Chen Fengyang , Tian Lin , Wang Jianguo , Yang Ni , Hou Yanqing , Huang Lingyun , Xie Gang
Published: May 2022

A study of boron impurities deposited on a Si(100) surface in a SiHCl3-BCl3-H2 system is reported in this paper, using periodic density functional theory with generalized gradient approximation (GGA). The results show that the discrete distances of BCl3 and SiHCl3 from the surface of the Si(100) unit cell are 1.873 Å and 2.340 Å, respectively, and the separation energies are −35.2549 kcal/mol and −10.64 kcal/mol, respectively. BCl3 and SiHCl3 are mainly adsorbed on the surface of the Si(100) unit cell in particular molecular orientations: the positive position and the hydrogen bottom-two-front position from the analysis of the bond length change and adsorption energy. The adsorption of SiHCl3 and BCl3 is accompanied by a charge transfer from the molecule to the surface of the unit cell of 0.24 and 0.29 eV, respectively. BCl3 reacts more readily than SiHCl3 with the Si(100) surface, resulting in the deposition of boron impurities on the polysilicon surface.
Satiety of Edible Insect-Based Food Products as a Component of Body Weight Control
Authors: Skotnicka Magdalena , Mazurek Aleksandra , Karwowska Kaja , Folwarski Marcin
Published: May 2022

Among the many aspects determining the nutritional potential of insect-based foods, research into the satiating potential of foods is an important starting point in the design of new functional foods, including those based on edible insects. The aim of this study was to assess the satiating value of products with the addition of freeze-dried insect flour. The test material included wheat pancakes in which corresponding proportions of wheat flour were substituted with 10% Mw, 0% Mw, and 30% Mw of flour from freeze-dried Tenebrio molitor, 10% Bw, 20% Bw, and 30% Bw of flour from Alphitobius diaperinus, and 10% Cr, 20% Cr, and 30% Cr of flour from Acheta domesticus. The study included the characterisation of physico-chemical properties and their effect on the satiating potential of the analysed pancakes. A total of 71 healthy volunteers (n = 39 women, n = 32 men) with no food phobias were qualified for the study. Each subject rated the level of hunger and satiety before and after ingestion at 30 min intervals over the subsequent 180 min on two separate graphical scales. The rating was done on an unstructured 100 mm visual analogue scale (VAS). A portion intended for testing had a value of 240 kcal. The highest average satiety values were noted for the pancakes with an addition of 30% Alphitobius diaperinus (Bw) and with the addition of 20% and 30% addition of Acheta domesticus flour (Cr). The Tenebrio molitor-based products were the least satiating. However, the largest addition of 30% of an insect flour for each variant considerably increased the satiating potential as compared to the control sample. Satiety was influenced the most by the protein content in the test wheat pancakes. The results support the idea of a possible usage of insect-based food products in the composition of obesity treatment diets, carbohydrate-limiting diets, and as alternative sources of protein.
Longitudinal Association of Dietary Energy Density with Abdominal Obesity among Chinese Adults from CHNS 1993–2018
Authors: Hu Haojie , Zuo Lijun , Song Xiaoyun , Su Chang , Wang Huijun , Zhang Bing , Ding Gangqiang
Published: May 2022

Few studies have explored the longitudinal association between dietary energy density and waist circumference and abdominal obesity in adults in China. This study aimed to analyze the relationship between dietary energy density and waist circumference and abdominal obesity in Chinese residents aged 18–64. Using data from the CHNS from 1993 to 2018, 25,817 adult residents aged 18 to 64 were selected for the study. Three consecutive 24-h dietary recalls and home-weighed seasonings were used to assess food intake. A multilevel model was used to analyze the relationship between dietary energy density and waist circumference and abdominal obesity. The results showed that compared with the lowest dietary energy density group, females had an increased risk of abdominal obesity (OR = 1.16, 95% CI: 1.05, 1.29), and females’ waist circumference increased significantly by 0.24 cm (95% CI: 0.39–1.09) in the highest dietary energy density group. No association between dietary energy density and waist circumference and abdominal obesity was observed in males. This study shows that higher dietary energy density is significantly associated with females’ waist circumference and abdominal obesity. Further research on high dietary energy density and abdominal obesity will provide scientific basis for the effective control of abdominal obesity.
Safety of Short-Term Treatments with Oral Chloroquine and Hydroxychloroquine in Patients with and without COVID-19: A Systematic Review
Authors: Marin Sergio , Martin Val Alba , Bosch Peligero Maite , Rodríguez-Bernuz Cristina , Pérez-Ricart Ariadna , Vilaró Jaques Laia , Paredes Roger , Roca Josep , Quiñones Carles
Published: May 2022

Chloroquine (CQ) and hydroxychloroquine (HCQ) have recently become the focus of global attention as possible treatments for Coronavirus Disease 2019 (COVID-19). The current systematic review aims to assess their safety in short treatments (≤14 days), whether used alone or in combination with other drugs. Following the PRISMA and SWiM recommendations, a search was conducted using four health databases for all relevant English-, Chinese-, and Spanish-language studies from inception through 30 July 2021. Patients treated for any condition and with any comparator were included. The outcomes of interest were early drug adverse effects and their frequency. A total of 254 articles met the inclusion criteria, including case and case-control reports as well as cross-sectional, cohort, and randomised studies. The results were summarised either qualitatively in table or narrative form or, when possible (99 studies), quantitatively in terms of adverse event frequencies. Quality evaluation was conducted using the CARE, STROBE, and JADAD tools. This systematic review showed that safety depended on drug indication. In COVID-19 patients, cardiac adverse effects, such as corrected QT interval prolongation, were relatively frequent (0–27.3% and up to 33% if combined with azithromycin), though the risk of torsade de pointes was low. Compared to non-COVID-19 patients, COVID-19 patients experienced a higher frequency of cardiac adverse effects regardless of the regimen used. Dermatological adverse effects affected 0–10% of patients with autoimmune diseases and COVID-19. A broad spectrum of neuropsychiatric adverse effects affected patients treated with CQ for malaria with variable frequencies and some cases were reported in COVID-19 patients. Gastrointestinal adverse effects occurred regardless of drug indication affecting 0–50% of patients. In conclusion, CQ and HCQ are two safe drugs widely used in the treatment of malaria and autoimmune diseases. However, recent findings on their cardiac and neuropsychiatric adverse effects should be considered if these drugs were to be proposed as antivirals again.
A Combination of Pharmacophore-Based Virtual Screening, Structure-Based Lead Optimization, and DFT Study for the Identification of S. epidermidis TcaR Inhibitors
Authors: Vuppala Srimai , Kim Jaeyoung , Joo Bo-Sun , Choi Ji-Myung , Jang Joonkyung
Published: May 2022

The transcriptional regulator (TcaR) enzyme plays an important role in biofilm formation. Prevention of TcaR-DNA complex formation leads to inhibit the biofilm formation is likely to reveal therapeutic ways for the treatment of bacterial infections. To identify the novel ligands for TcaR and to provide a new idea for drug design, two efficient drug design methods, such as pharmacophore modeling and structure-based drug design, were used for virtual screening of database and lead optimization, respectively. Gemifloxacin (FDA-approved drug) was considered to generate the pharmacophore model for virtual screening of the ZINC database, and five hits, namely ZINC77906236, ZINC09550296, ZINC77906466, ZINC09751390, and ZINC01269201, were identified as novel inhibitors of TcaR with better binding energies. Using structure-based drug design, a set of 7a–7p inhibitors of S. epidermidis were considered, and Mol34 was identified with good binding energy and high fitness score with improved pharmacological properties. The active site residues ARG110, ASN20, HIS42, ASN45, ALA38, VAL63, VAL68, ALA24, VAL43, ILE57, and ARG71 are playing a promising role in inhibition process. In addition, we performed DFT simulations of final hits to understand the electronic properties and their significant role in driving the inhibitor to adopt apposite bioactive conformations in the active site. Conclusively, the newly identified and designed hits from both the methods are promising inhibitors of TcaR, which can hinder biofilm formation.
Controlled-Release Nanosystems with a Dual Function of Targeted Therapy and Radiotherapy in Colorectal Cancer
Authors: Cruz-Nova Pedro , Ancira-Cortez Alejandra , Ferro-Flores Guillermina , Ocampo-García Blanca , Gibbens-Bandala Brenda
Published: May 2022

Nanoparticles are excellent platforms for several biomedical applications, including cancer treatment. They can incorporate different molecules to produce combinations of chemotherapeutic agents, radionuclides, and targeting molecules to improve the therapeutic strategies against cancer. These specific nanosystems are designed to have minimal side effects on healthy cells and better treatment efficacy against cancer cells when compared to chemotherapeutics, external irradiation, or targeted radiotherapy alone. In colorectal cancer, some metal and polymeric nanoparticle platforms have been used to potentialize external radiation therapy and targeted drug delivery. Polymeric nanoparticles, liposomes, albumin-based nanoparticles, etc., conjugated with PEG and/or HLA, can be excellent platforms to increase blood circulation time and decrease side effects, in addition to the combination of chemo/radiotherapy, which increases therapeutic efficacy. Additionally, radiolabeled nanoparticles have been conjugated to target specific tissues and are mainly used as agents for diagnosis, drug/gene delivery systems, or plasmonic photothermal therapy enhancers. This review aims to analyze how nanosystems are shaping combinatorial therapy and evaluate their status in the treatment of colorectal cancer.