bluuebunny/crossref_metadata_2025 huggingface dataset:
Resting Physiologic Dead Space as Predictor of Postoperative Pulmonary Complications After Robotic-Assisted Lung Resection: A Pilot Study
Authors: Godbole Rohit , Church Sanford B. , Abolhoda Amir , Porszasz Janos , Sassoon Catherine S. H.
Published: July 2022

Lung resection surgery carries significant risks of postoperative pulmonary complications (PPC). Cardiopulmonary exercise testing (CPET) is performed to predict risk of PPC in patients with severely reduced predicted postoperative forced expiratory volume in one second (FEV1) and diffusion of carbon monoxide (DLCO). Recently, resting end-tidal partial pressure of carbon dioxide (PETCO2) has been shown as a good predictor for increased risk of PPC. However, breath-breath breathing pattern significantly affects PETCO2. Resting physiologic dead space (VD), and physiologic dead space to tidal volume ratio (VD/VT), may be a better predictor of PPC than PETCO2. The objective of this study was to prospectively determine the utility of resting measurements of VD and VD/VT in predicting PPC in patients who underwent robotic-assisted lung resection for suspected or biopsy-proven lung malignancy. Thirty-five consecutive patients were included in the study. Patients underwent preoperative pulmonary function testing, symptom-limited CPET, and a 6-min walk test. In the first 2 min prior to the exercise portion of the CPET, we obtained resting VT, minute ventilation (V˙E), VD (less instrument dead space), VD/VT, PETCO2, and arterial blood gases. PPC within 90 days were recorded. Fourteen (40%) patients had one or more PPC. Patients with PPC had significantly elevated resting VD compared to those without (0.318 ± 0.028 L vs. 0.230 ± 0.017 L (± SE), p < 0.006), and a trend toward increased VD/VT (0.35 ± 0.02 vs. 0.31 ± 0.02, p = 0.051). Area under the receiver operating characteristic (ROC) for VD was 0.81 (p < 0.002), VD/VT was 0.68 (p = 0.077), and PETCO2 was 0.52 (p = 0.840). Peak V˙O2, V˙E/ V˙CO2 slope, pulmonary function tests, 6-min walk distance and arterial blood gases were similar between the two groups. Intensive care unit and total hospital length of stay was significantly longer in those with PPC. In conclusion, preoperative resting VD was significantly elevated in patients with PPC. The observed increase in resting VD may be a potentially useful predictor of PPC in patients undergoing robotic-assisted lung resection surgery for suspected or biopsy-proven lung malignancy. A large prospective study is needed for confirmation.
The Central Circadian Clock Protein TaCCA1 Regulates Seedling Growth and Spike Development in Wheat (Triticum aestivum L.)
Authors: Gong Jie , Tang Yimiao , Liu Yongjie , Sun Renwei , Li Yanhong , Ma Jinxiu , Zhang Shengquan , Zhang Fengting , Chen Zhaobo , Liao Xiangzheng , Sun Hui , Lu Zefu , Zhao Changping , Gao Shiqing
Published: July 2022

The biological functions of the circadian clock on growth and development have been well elucidated in model plants, while its regulatory roles in crop species, especially the roles on yield-related traits, are poorly understood. In this study, we characterized the core clock gene CIRCADIAN CLOCK-ASSOCIATED 1 (CCA1) homoeologs in wheat and studied their biological functions in seedling growth and spike development. TaCCA1 homoeologs exhibit typical diurnal expression patterns, which are positively regulated by rhythmic histone modifications including histone H3 lysine 4 trimethylation (H3K4me3), histone H3 lysine 9 acetylation (H3K9Ac), and histone H3 lysine 36 trimethylation (H3K36me3). TaCCA1s are preferentially located in the nucleus and tend to form both homo- and heterodimers. TaCCA1 overexpression (TaCCA1-OE) transgenic wheat plants show disrupted circadian rhythmicity coupling with reduced chlorophyll and starch content, as well as biomass at seedling stage, also decreased spike length, grain number per spike, and grain size at the ripening stage. Further studies using DNA affinity purification followed by deep sequencing [DNA affinity purification and sequencing (DAP-seq)] indicated that TaCCA1 preferentially binds to sequences similarly to “evening elements” (EE) motif in the wheat genome, particularly genes associated with photosynthesis, carbon utilization, and auxin homeostasis, and decreased transcriptional levels of these target genes are observed in TaCCA1-OE transgenic wheat plants. Collectively, our study provides novel insights into a circadian-mediated mechanism of gene regulation to coordinate photosynthetic and metabolic activities in wheat, which is important for optimal plant growth and crop yield formation.
Physiological and Biochemical Responses of Pepper (Capsicum annuum L.) Seedlings to Nickel Toxicity
Authors: Altaf Muhammad Ahsan , Hao Yuanyuan , He Chengyao , Mumtaz Muhammad Ali , Shu Huangying , Fu Huizhen , Wang Zhiwei
Published: July 2022

Globally, heavy metal pollution of soil has remained a problem for food security and human health, having a significant impact on crop productivity. In agricultural environments, nickel (Ni) is becoming a hazardous element. The present study was performed to characterize the toxicity symptoms of Ni in pepper seedlings exposed to different concentrations of Ni. Four-week-old pepper seedlings were grown under hydroponic conditions using seven Ni concentrations (0, 10, 20, 30, 50, 75, and 100 mg L–1NiCl2. 6H2O). The Ni toxicity showed symptoms, such as chlorosis of young leaves. Excess Ni reduced growth and biomass production, root morphology, gas exchange elements, pigment molecules, and photosystem function. The growth tolerance index (GTI) was reduced by 88-, 75-, 60-, 45-, 30-, and 19% in plants against 10, 20, 30, 50, 75, and 100 mg L–1Ni, respectively. Higher Ni concentrations enhanced antioxidant enzyme activity, ROS accumulation, membrane integrity [malondialdehyde (MDA) and electrolyte leakage (EL)], and metabolites (proline, soluble sugars, total phenols, and flavonoids) in pepper leaves. Furthermore, increased Ni supply enhanced the Ni content in pepper’s leaves and roots, but declined nitrogen (N), potassium (K), and phosphorus (P) levels dramatically. The translocation of Ni from root to shoot increased from 0.339 to 0.715 after being treated with 10–100 mg L–1Ni. The uptake of Ni in roots was reported to be higher than that in shoots. Generally, all Ni levels had a detrimental impact on enzyme activity and led to cell death in pepper seedlings. However, the present investigation revealed that Ni ≥ 30 mg L–1lead to a deleterious impact on pepper seedlings. In the future, research is needed to further explore the mechanism and gene expression involved in cell death caused by Ni toxicity in pepper plants.
The Different Classification of Hospitals Impact on Medical Outcomes of Patients in China
Authors: Li Lele , Du Tiantian , Zeng Siyu
Published: July 2022

Background

In China, different classification of hospitals (COH) provide treatment for patients with different degrees of illness. COH play an important role in Chinese medical outcomes, but there is a lack of quantitative description of how much impact the results have. The objective of this study is to examine the correlation between COH on medical outcomes with the hope of providing insights into appropriate care and resource allocation.

Methods

From the perspective of the COH framework, using the Urban Employee Basic Medical Insurance (UEBMI) data of Chengdu City from 2011 to 2015, with a sample size of 512,658 hospitalized patients, this study used the nested multinomial logit model (NMNL) to estimate the impact of COH on the medical outcomes.

Results

The patients were mainly elderly, with an average age of 66.28 years old. The average length of stay was 9.61 days. The female and male gender were split evenly. A high level of hospitals is positively and significantly associated with the death and transfer rates (p < 0.001), which may be related to more severe illness among patients in high COH.

Conclusion

The COH made a difference in the medical outcomes significantly. COH should be reasonably selected according to disease types to achieve the optimal medical outcome. So, China should promote the construction of a tiered delivery system.
Prevalence and Genotype Distribution of Human Papillomavirus Infection Among 40,613 Women: An Outpatient-Based Population Study in Kunming, Yunnan
Authors: Zhang Yu , Xu Ya , Dian Ziqin , Zhang Guiqian , Fan Xin , Zhao Yuan , Sun Yi
Published: July 2022

Human Papillomavirus (HPV) infection is one of the most common sexually transmitted infections worldwide. The current study aimed to investigate the prevalence and genotype distribution of HPV infection among outpatient-based populations. A total of 40,613 women were recruited from the First People's Hospital of the Yunnan Province. Our study retrospectively analyzed the results of cervical HPV screening among 40,613 women. The results of study showed the prevalence and genotype distribution of HPV infection was different among various outpatient-based populations, and the prevalence of HPV infection was the highest in the gynecological outpatients (26.59%), followed by reproductive gynecological outpatients (18.51%), and the prevalence of physically examined population was the lowest (8.15%). The outpatient-based population was facing a huge threat of HPV infection, especially women from the gynecology clinic. The three most common HPV genotypes were HPV-52 (4.79%), 16 (2.95%) and 58 (2.83%). However, the distribution of HPV genotype varied by populations, especially in physically examined population, the infection rate of HPV-81 ranked third among all infections with various genotypes. Two peaks of prevalence of HPV infection were observed among women under 25 years (31.93%) and over 55 years (28.55%), while the prevalence in women aged 46–55 years (20.18%) was the lowest. Our study on the prevalence and genotype distribution of HPV infection among various outpatient-based populations will provide scientific evidence for vaccination strategies of HPV and prevention and control plans of cervical cancer in Kunming area.
Patient safety culture awareness among healthcare providers in a tertiary hospital in Riyadh, Saudi Arabia
Authors: Alsulami Abdulkarim , A'aqoulah Ashraf , Almutairi Nouf
Published: July 2022

Patient safety is a serious concern in the health care industry. To enhance patient safety, healthcare providers are expected to minimize accidental harm to patients and enhance the quality of patient-centered care. The main objective of this study is to explore the awareness of the patient safety culture among healthcare providers. It is further intended to assess key fields and factors that hinder patient safety adoption and determine the effects of demographic factors on healthcare providers' awareness of patient safety culture. This study applied a cross-sectional quantitative design. It was conducted in a tertiary hospital in Riyadh, Saudi Arabia. The participants consisted of all healthcare providers working in a specific tertiary hospital in Saudi Arabia. A random sampling technique was applied in this study. The study sample size was 409 participants. A valid and reliable questionnaire was used to collect the required data. The T-tests, ANOVA. And regression was used. The study found that there is a moderate level of patient safety culture awareness among healthcare providers. Moreover, the findings also revealed that the age group “31–40” showed statistically different awareness levels as compared to the “more than 50 years' age group” (p = 0.012). Also, this study has found that gender and education have a significant influence on the awareness level of patient safety culture while position and work area have no influence on the awareness level of patient safety culture among healthcare providers. Managers in healthcare institutions should develop speeder response plans and make them part of the patient safety culture. Institutions offering bachelor's degrees and postgraduate in nursing should pay more attention to the subject of patient safety. The government healthcare sector, together with the private healthcare sector, should continuously train healthcare providers on patient safety procedures to improve the patient safety culture. Healthcare providers should be encouraged to report errors made during diagnosis or treatments to avoid them in the future.
Online Brand Community User Segments: A Text Mining Approach
Authors: Ge Ruichen , Zhao Hong , Zhang Sha
Published: July 2022

There is a trend that customers increasingly join the online brand community. However, evidence shows that there are nuances between different user segments, and only a small group of users are active. Thus, one key concern marketers face is identifying and targeting specific segments and decreasing user churn rates in an online environment. To this end, this study aims to propose a UGC-based segmentation of online brand community users, identify the characteristics of each segment, and consequently reduce online brand community users' churn rate. We used python to obtain users' post data from a well-known online brand community in China between July 2012 and December 2019, resulting in 912,452 posts and 20,493 users. We then use text mining and clustering methods to segment the users and compare the differences between the segments. Three groups—information-oriented users, entertainment-oriented users, and multi-motivation users—were emerged. Our results imply that entertainment-oriented users were the most active, yet, multi-directional users have the lowest probability of churn, with a churn rate of only 0.607 times than that of users who focus either on information or entertainment. Implications for marketing and future research opportunities are discussed.
Changes in a Bottom-Up Vehicular Emissions Inventory and Its Impact on Air Pollution During COVID-19 Lockdown in São Paulo, Brazil
Authors: Ibarra-Espinosa Sergio , Rehbein Amanda , Dias de Freitas Edmilson , Martins Leila , Andrade Maria de Fatima , Landulfo Eduardo
Published: July 2022

Due to coronavirus disease 2019 (COVID-19), many cities implemented strict lockdown to stop the spread of this new disease. Consequently, it was reported lower levels of air pollution due to less human activity outdoors. The changes were registered using surface monitoring stations or satellite observations. However, modeling those environmental changes has remained a challenge because of our limitations in the emissions estimation and also, for the numerical modeling itself. In this study, the vehicular emissions were estimated for March 2020 in the megacity of São Paulo using the Vehicular Emissions INventory model (VEIN). The emissions estimation showed an increment of VOC/NO2 downtown, due to the decrease in circulation of urban transportation and light vehicles. Then, a set of Weather Research and Forecasting models with chemistry (WRF-Chem) simulations were performed with different chemical mechanisms and initial conditions. The modeled diurnal cycles represent the variations observed in March 2020 for the periods pre-lockdown, transition, and lockdown. However, it is imperative to include other sources than vehicular to have a local and comprehensive emissions inventory.
Construction and Application of Standardized Postoperative Pain-Management Procedure for Patients With Perianal Abscess: A Retrospective Study
Authors: Wang Xiu-Mei , Jiang Wei-Lian , Ma Li-Fang , Guo Yue , Cui Li-Ping , Niu Yan-Bin
Published: July 2022

Objective

The present study explored the construction and application of a standardized postoperative pain-management procedure for patients with perianal abscess.

Methods

Two study groups (the observation group and the intervention group) were established retrospectively. The observation group comprised 46 patients with perianal abscess who enrolled in this study between June 2019 and June 2020. The intervention group comprised 48 patients who enrolled in the study between July 2020 and July 2021. All patients were enrolled using the convenience sampling method. A pain-management team was established, and standardized procedure management was implemented in the intervention group, while routine pain management was implemented in the observation group. Indices related to the patients' postoperative pain-control satisfaction and rehabilitation were compared between the two groups.

Results

The patients' pain-control satisfaction, wound edema score, edema disappearance time, urinary retention, and defecation difficulty following intervention were better in the intervention group than in the observation group, and the differences were statistically significant (P < 0.05 for all).

Conclusion

The implementation of the standardized postoperative pain-management procedure in patients with perianal abscess can effectively improve the patient's level of pain and satisfaction and promote rehabilitation.
Fluorescence-guided lung nodule identification during minimally invasive lung resections
Authors: Tajè Riccardo , Gallina Filippo Tommaso , Forcella Daniele , Vallati Giulio Eugenio , Cappelli Federico , Pierconti Federico , Visca Paolo , Melis Enrico , Facciolo Francesco
Published: July 2022

In the last few years, minimally invasive surgery has become the standard routine practice to manage lung nodules. Particularly in the case of robotic thoracic surgery, the identification of the lung nodules that do not surface on the visceral pleura could be challenging. Therefore, together with the evolution of surgical instruments to provide the best option in terms of invasiveness, lung nodule localization techniques should be improved to achieve the best outcomes in terms of safety and sensibility. In this review, we aim to overview all principal techniques used to detect the lung nodules that do not present the visceral pleura retraction. We investigate the accuracy of fluorescence guided thoracic surgery in nodule detection and the differences among the most common tracers used.
About the Grinding of Gear Hob’s Rake Face
Authors: Hodgyai Norbert , Drăgoi Mircea Viorel , Tolvaly-Roşca Ferenc , Máté Márton
Published: July 2022

The most simple and robust construction of the monolithic gear hobs present a common helical rake face for a given line of teeth, whose generatrix is a straight-line segment perpendicular to the hob’s axis while its directory is a helix, perpendicular to the pitch helix. As a consequence, constructive rake angles are zero on all edges. Total curvatures of such a surface are negative. Thus, it can be grinded only using the conical surface of a platter type grinding wheel, or a grinding bit. Despite this, some industry practices, possibly for reasons of simplicity and cost lowering, involve the plain grinding surface, supposed to a helical motion. This paper deals with the CAD-simulation of the grinding process using the plain wheel surface, and it shows the differences between the theoretical and rake face and the real obtained helical surface.
Adaptive Deployable Thin Spherical Shell Reflectors
Authors: Nielsen Carl Johan G. , Tian Dalong , Wang Kainan , Preumont André
Published: July 2022

This paper begins with a quick survey of potential space applications and a brief review of previous experiments on the shape control of a spherical shell reflector with a thin film of PVDF-TrFE. Next, the problem of thermal sensitivity is addressed numerically; it is found that, because of the large thermal expansion of the active material, the surface figure error generated by a linear thermal gradient on a unimorph reflector is considerable and its correction requires large control voltages. The surface figure accuracy can be greatly improved by a balanced design (i.e., adding a passive layer symmetrical to the PVDF-TrFE layer) and using a low CTE substrate. Finally, the paper considers a petal reflector; the unimorph design is even more sensitive than the full reflector to the thermal gradient, but the balanced design turns out to be better than the full reflector, both from the point of view of the surface figure error and the control voltages.
Influence of Synthetic Jets on Multiscale Features in Wall-Bounded Turbulence
Authors: Li Biaohui , Zhang Jinhao , Jiang Nan
Published: July 2022

This experimental research focuses on the impacts of submerged synthetic jets on a fully-developed turbulent boundary layer (TBL) under a drag reduction working case. Two-dimensional velocity vectors in the flow field are captured with the aid of a particle image velocimetry (PIV) system. Proper orthogonal decomposition (POD) analyses provide evidence that synthetic jets notably attenuate the induction effect of prograde vortex on the low-speed fluid in large-scale fluctuation velocity field, thereby weakening the bursting process of near-wall turbulent events. Furthermore, the introduced perturbance redistributes the turbulent kinetic energy (TKE) and concentrates the TKE onto small-scale coherent structures. Modal time coefficients in various orders of POD are divided into components of multiple frequency bands by virtue of complementary ensemble empirical mode decomposition (CEEMD). It is found that the turbulence signals are shifted from low-frequency to high-frequency bands thanks to synthetic jets, thus revealing the relationship between scales and frequency bands. One further method of scale decomposition is proposed, that is, the large-scale fluctuating flow field will be obtained after removing the high-frequency noise data with the help of continuous mean square error (CMSE) criterion.
OpenAP.top: Open Flight Trajectory Optimization for Air Transport and Sustainability Research
Authors: Sun Junzi
Published: July 2022

Trajectory optimization has been an active area of research for air transport studies for several decades. But almost all flight optimizers proposed in the literature remain close-sourced, which presents a major disadvantage for the advancement of scientific research. This optimization depends on aircraft performance models, emission models, and operational constraints. In this paper, I present a fully open trajectory optimizer, OpenAP.top, which offers researchers easy access to the complex but efficient non-linear optimal control approach. Full flights can be generated without specifying flight phases, and specific flight segments can also be independently created. The optimizer adapts to meteorological conditions and includes conventional fuel and cost index objectives. Based on global warming and temperature potentials, its climate objectives form the basis for climate optimal air transport studies. The optimizer’s performance and uncertainties under different factors like varying mass, cost index, and wind conditions are analyzed. Overall, this new optimizer brings a high performance for optimal trajectory generations by providing four-dimensional and wind-enabled full-phase optimal trajectories in a few seconds.
Grazing Horse Effects on Desert Grassland Soil Gross Nitrification and Denitrification Rates in Northern China
Authors: Wang Xiaonan , Wang Chengjie , Zhou Chengyang , Zuo Shining , Ji Yixin , Lamao Qiezhuo , Huang Ding
Published: July 2022

The aim of this study was to investigate the effects of grazing on soil gross nitrification (GN) and denitrification (DN) rates and soil environmental factors on GN and DN in the desert grassland of northern China. Soil samples were collected from July to November in 2017 and March to June in 2018, with 5-5 soil samples taken from three enclosures (CK) vs. three heavy-grazing (G) randomized treatment blocks. We determined: (1) the soil moisture (SM), pH, bulk density (BD), total nitrogen (TN), soil organic carbon (SOC), and inorganic nitrogen (IN, NH4+-N, and NO3−-N) content, and (2) GN and DN. The relationship between the changes in GN, DN, and the soil environment was analyzed using stepwise multiple-regression analysis. Gross nitrification, DN, pH, BD, C/N, SM, IN, and NO3−-N varied significantly by month. Grazing induced significant increases in SM and NO3− only. GN in the CK treatment was related to NH4+-N and NO3−-N, while GN in the G treatment was related to NH4+-N and SM. DN in the CK treatment was related to NH4+-N, while DN in the G treatment was related to C/N. Additionally, GN and DN had obvious seasonal variations and reached a maximum in July. This highlights the different underlying mechanisms that affect soil GN and DN and the dynamics, particularly in the desert grassland system.
Image Segmentation of UAV Fruit Tree Canopy in a Natural Illumination Environment
Authors: Lu Zhongao , Qi Lijun , Zhang Hao , Wan Junjie , Zhou Jiarui
Published: July 2022

Obtaining canopy area, crown width, position, and other information from UAV aerial images and adjusting spray parameters in real-time according to this information is an important way to achieve precise pesticide application in orchards. However, the natural illumination environment in the orchard makes extracting the fruit tree canopy difficult. Hereto, an effective unsupervised image segmentation method is developed in this paper for fast fruit tree canopy acquisition from UAV images under natural illumination conditions. Firstly, the image is preprocessed using the shadow region luminance compensation method (SRLCM) that is proposed in this paper to reduce the interference of shadow areas. Then, use Naive Bayes to obtain multiple high-quality color features from 10 color models was combined with ensemble clustering to complete image segmentation. The segmentation experiments were performed on the collected apple tree images. The results show that the proposed method’s average precision rate, recall rate, and F1-score are 95.30%, 84.45%, and 89.53%, respectively, and the segmentation quality is significantly better than ordinary K-means and GMM algorithms.
Development of an Energy Efficient and Fully Autonomous Low-Cost IoT System for Irrigation Scheduling in Water-Scarce Areas Using Different Water Sources
Authors: Tsiropoulos Zisis , Skoubris Evangelos , Fountas Spyros , Gravalos Ioannis , Gemtos Theofanis
Published: July 2022

Politicians and the general public are concerned about climate change, water scarcity, and the constant reduction in agricultural land. Water reserves are scarce in many regions in the world, negatively affecting agricultural productivity, which makes it a necessity to introduce sustainable water resource management. Nowadays, there is a number of commercial IoT systems for irrigation scheduling, helping farmers to manage and save water. However, these systems focus on using the available fresh water sources, without being able to manage alternative water sources. In this study, an Arduino-based low-cost IoT system for automated irrigation scheduling is developed and implemented, which can provide measurements of water parameters with high precision using low-cost sensors. The system used weather station data combined with the FAO56 model for computing the water requirements for various crops, and it was capable of handling and monitoring different water streams by supervising their quality and quantity. The developed IoT system was tested in several field trials, to evaluate its capabilities and functionalities, including the sensors’ accuracy, its autonomous controlling and operation, and its power consumption. The results of this study show that the system worked efficiently on the management and monitoring of different types of water sources (rainwater, groundwater, seawater, and wastewater) and on automating the irrigation scheduling. In addition, it was proved that the system is can be used for long periods of time without any power source, making it ideal for using it on annual crops.
Mechanical Characteristics of Rice Root–Soil Complex in Rice–Wheat Rotation Area
Authors: Zhu Huibin , Zhao Haoran , Bai Lizhen , Ma Shi’ao , Zhang Xu , Li Hui
Published: July 2022

In order to explore the mechanical characteristics of stubble breaking and provide a theoretical basis for the design of a stubble breaking and crushing blockage prevention device, an orthogonal test with three factors (water content, bulk weight, and root content) and a quick shearing test of remolded soil were carried out in a laboratory. The shear resistance of the rice root–soil complex was studied and the soil mechanical equation of the rice root–soil composite was established. It is found that the shear strength of the root–soil composite is related to water content and root content. When the water content was around 30% and the root content was 1.1%, the cohesion of the root–soil composite was the smallest. With the decrease or increase of water content and the decrease or increase of root content, the cohesion of the root–soil composite showed the trend of increasing layer by layer. When the water content was 40% and the root content was 1.1%, the internal friction angle of the root–soil composite showed the minimum value. With the decrease of water content and the increase of root content, the internal friction angle of the root–soil composite gradually showed an increasing trend; while the root content had a great influence on the internal friction angle, the influence of water content on it was relatively small. The direct shear and fast shear tests of root–soil composite samples showed that the shear strength of the root–soil composite and the normal pressure loaded on it conform to the Coulomb equation. The presence of roots increased the shear strength and cohesion value of the soil and improved the resistance to deformation of the soil, but had little influence on the internal friction angle.
Rural Agrotourism Development Strategies in Less Favored Areas: The Case of Hacienda Guachinango de Trinidad
Authors: Pelegrín Entenza Norberto , Vázquez Pérez Antonio , Pelegrín Naranjo Analién
Published: July 2022

The objective of the work was to assess and identify potential perspectives for the development of agrotourism activity in the Hacienda Guachinango del Valle de los Ingenios in the Trinidad municipality, so that the methodology applied and the results achieved can serve as a reference for application in other similar contexts in the country and in the Central American area. For this, the analysis and synthesis of spatial elements, discussion groups, the interpretation of descriptive statistics, and the study of the natural and social environment were applied. The diagnosis of resources was carried out in this work to determine the degree of competitiveness and attraction of the agrotourism product, and it was possible to establish the most convenient strategies to follow, considering the real possibilities of prospective development of the place studied. It was concluded that the proposal to develop agrotourism in Hacienda Guachinango constitutes a viable option to design and introduce a strategy for growth and socioeconomic reactivation based on the use of the natural, historical, cultural, and social potentialities of the small families dedicated to agricultural work.
Advances in Barley Breeding for Improving Nitrogen Use Efficiency
Authors: Chen Zhiwei , Li Luli , Halford Nigel G. , Xu Hongwei , Huang Linli , Gao Runhong , Lu Ruiju , Liu Chenghong
Published: July 2022

Crop breeding for high nitrogen use efficiency (NUE) or tolerance to low nitrogen fertilization is thought to be an ideal solution to reduce the cost, carbon footprint, and other environmental problems caused by the excess use of nitrogen fertilizers. As a model plant for cereal crops, barley has many advantages, including good adaptability, a short growth period, and high natural stress resistance or tolerance. Therefore, research on improving NUE in barley is not only beneficial for nitrogen-efficient barley breeding but will also inform NUE improvement in other cereal crops. In this review, recent progress in understanding barley’s response to nitrogen nutrition, evaluation of NUE or low-nitrogen tolerance, quantitative trait loci (QTL) mapping and gene cloning associated with improving NUE, and breeding of nitrogen-efficient barley is summarized. Furthermore, several biotechnological tools that could be used for revealing the molecular mechanisms of NUE or breeding for improving NUE in barley are introduced, including GWAS, omics, and gene editing. The latest research ideas in unraveling the molecular mechanisms of improving NUE in other crops are also discussed. Thus, this review provides a better understanding of improving the NUE of barley and some directions for future research in this area.
Fatal Tractor Accidents in the Agricultural Sector in Spain during the Past Decade
Authors: Jarén Carmen , Ibarrola Alicia , Mangado Txuma , Adin Aritz , Arnal Pedro , López-Maestresalas Ainara , Ríos Alonso , Arazuri Silvia
Published: July 2022

Currently, there is a discrepancy between the number of occupational accidents in the agricultural sector reported by Spanish governmental sources and those actually occurring in general. This is mainly due to the official definition of ‘occupational accident’ in the current regulations. In order to be able to analyse all fatal accidents involving tractors, other sources of information must therefore be used. In this study, we have collected the news published in different media during the period 2010–2019. Statistical models that take into account the spatial and temporal dependence of the data were used to estimate the rates of fatal accidents in the provinces of Spain using the Bayesian inference technique INLA (Integrated Nested Laplace Approximation). The results obtained showed that the total number of fatal accidents in that period was 644. The crude rates of fatal accidents per province ranged from 0 to 223.5 fatal accidents per 100,000 registered tractors. In addition, the overall rate for Spain as a whole was 6.87 fatal accidents per 100,000 tractors. As in other EU countries, it was found that the regions with the highest number of accidents were also related to steep terrain, to an older tractor fleet and to horticultural crops and vineyards.
Beneficial Shifts in the Gut Bacterial Community of Gilthead Seabream (Sparus aurata) Juveniles Supplemented with Allium-Derived Compound Propyl Propane Thiosulfonate (PTSO)
Authors: Rabelo-Ruiz Miguel , Newman-Portela Antonio M. , Peralta-Sánchez Juan Manuel , Martín-Platero Antonio Manuel , Agraso María del Mar , Bermúdez Laura , Aguinaga María Arántzazu , Baños Alberto , Maqueda Mercedes , Valdivia Eva , Martínez-Bueno Manuel
Published: July 2022

This study analyzes the potential use of an Allium-derived compound, propyl propane thiosulfonate (PTSO), as a functional feed additive in aquaculture. Gilthead seabream (Sparus aurata) juveniles had their diet supplemented with this Allium-derived compound (150 mg/kg of PTSO) and were compared with control fish. The effects of this organosulfur compound were tested by measuring the body weight and analyzing the gut microbiota after 12 weeks. The relative abundance of potentially pathogenic Vibrio and Pseudomonas in the foregut and hindgut of supplemented fish significantly decreased, while potentially beneficial Lactobacillus increased compared to in the control fish. Shannon’s alpha diversity index significantly increased in both gut regions of fish fed with a PTSO-supplemented diet. Regarding beta diversity, significant differences between treatments only appeared in the hindgut when minority ASVs were taken into account. No differences occurred in body weight during the experiment. These results indicate that supplementing the diet with Allium-derived PTSO produced beneficial changes in the intestinal microbiota while maintaining the productive parameters of gilthead seabream juveniles.
Quality of Life within Horse Welfare Assessment Tools: Informing Decisions for Chronically Ill and Geriatric Horses
Authors: Long Mariessa , Dürnberger Christian , Jenner Florien , Kelemen Zsófia , Auer Ulrike , Grimm Herwig
Published: July 2022

Equine Quality of Life (QoL) is an important concern in decision making in veterinary medicine and is especially relevant for chronically ill or geriatric horses towards the end of their lives. To our knowledge, there is no currently available QoL assessment tool for chronically ill or geriatric horses that assesses equine QoL defined as the horse’s evaluation of their life. However, tools exist to assess equine welfare in different contexts. Hence, the aims of this study were to analyse how equine welfare, QoL, well-being and happiness assessment tools label, define and operationalise the concepts and to discuss the tools’ suitability to assess equine QoL in the context of end-of-life decisions for chronically ill or geriatric horses. Fourteen articles were found through a systematic literature search, describing ten equine welfare assessment tools and one approach to integrating equine QoL in veterinary practice that suggests QoL assessment parameters. We discuss that some welfare assessment tools have the potential to support the development of a QoL assessment tool informing decisions towards the end of horses’ lives if they are adjusted to focus on the horses’ experiences, to provide an integration into an overall QoL grade and are tailored to chronically ill or geriatric horses.
Routine Decontamination of Surfaces Relevant to Working Dogs: Neutralization of Superficial Coronavirus Contamination
Authors: Grady Sarah L. , Sebeck Natalie M. , Theodore Mellisa , Meidenbauer Karen L.
Published: July 2022

Given the increased deployment of working dogs to settings with pathogenic biological agents, a safe, effective, and logistically feasible surface decontamination protocol is essential to protect both the animals and their human handlers. Our group previously found that superficial contamination on surfaces relevant to the working dog community, including leashes and toys, could be significantly reduced using a standardized wiping protocol with various cleansing products. To expand upon this work, we analyzed the ability of this protocol to decontaminate surface-deposited bovine coronavirus, which was used as a BSL2 surrogate for SARS-CoV-2. Unsurprisingly, the physical characteristics of a given surface, including porosity and texture, had a significant effect on the ability to recover viable virus remaining on the surface post treatment. After correcting for these differences, however, wiping with 70% isopropyl alcohol (IPA) and 0.5% chlorhexidine performed best, reducing viral titers by >3 log on plastic bumper toys and nylon collars, and by >2 log on rubber toys and tennis balls. Leather leashes and Velcro proved more difficult to decontaminate, but both still showed significant loss of viral contamination following wiping with IPA or chlorhexidine. This work (i) validates the utility of a simple protocol for the neutralization of viruses on several surfaces, (ii) identifies materials that are more difficult to decontaminate, which should, thus, be considered for removal from field use, and (iii) highlights the need for further development of protocols testing porous or textured surfaces.
One-Step In Vitro Generation of ETV2-Null Pig Embryos
Authors: Moya-Jódar Marta , Coppiello Giulia , Rodríguez-Madoz Juan Roberto , Abizanda Gloria , Barlabé Paula , Vilas-Zornoza Amaia , Ullate-Agote Asier , Luongo Chiara , Rodríguez-Tobón Ernesto , Navarro-Serna Sergio , París-Oller Evelyne , Oficialdegui Maria , Carvajal-Vergara Xonia , Ordovás Laura , Prósper Felipe , García-Vázquez Francisco Alberto , Aranguren Xabier L.
Published: July 2022

Each year, tens of thousands of people worldwide die of end-stage organ failure due to the limited availability of organs for use in transplantation. To meet this clinical demand, one of the last frontiers of regenerative medicine is the generation of humanized organs in pigs from pluripotent stem cells (PSCs) via blastocyst complementation. For this, organ-disabled pig models are needed. As endothelial cells (ECs) play a critical role in xenotransplantation rejection in every organ, we aimed to produce hematoendothelial-disabled pig embryos targeting the master transcription factor ETV2 via CRISPR-Cas9-mediated genome modification. In this study, we designed five different guide RNAs (gRNAs) against the DNA-binding domain of the porcine ETV2 gene, which were tested on porcine fibroblasts in vitro. Four out of five guides showed cleavage capacity and, subsequently, these four guides were microinjected individually as ribonucleoprotein complexes (RNPs) into one-cell-stage porcine embryos. Next, we combined the two gRNAs that showed the highest targeting efficiency and microinjected them at higher concentrations. Under these conditions, we significantly improved the rate of biallelic mutation. Hence, here, we describe an efficient one-step method for the generation of hematoendothelial-disabled pig embryos via CRISPR-Cas9 microinjection in zygotes. This model could be used in experimentation related to the in vivo generation of humanized organs.
Prediction of Acute and Chronic Mastitis in Dairy Cows Based on Somatic Cell Score and Mid-Infrared Spectroscopy of Milk
Authors: Rienesl Lisa , Khayatzdadeh Negar , Köck Astrid , Egger-Danner Christa , Gengler Nicolas , Grelet Clément , Dale Laura Monica , Werner Andreas , Auer Franz-Josef , Leblois Julie , Sölkner Johann
Published: July 2022

Monitoring for mastitis on dairy farms is of particular importance, as it is one of the most prevalent bovine diseases. A commonly used indicator for mastitis monitoring is somatic cell count. A supplementary tool to predict mastitis risk may be mid-infrared (MIR) spectroscopy of milk. Because bovine health status can affect milk composition, this technique is already routinely used to determine standard milk components. The aim of the present study was to compare the performance of models to predict clinical mastitis based on MIR spectral data and/or somatic cell count score (SCS), and to explore differences of prediction accuracies for acute and chronic clinical mastitis diagnoses. Test-day data of the routine Austrian milk recording system and diagnosis data of its health monitoring, from 59,002 cows of the breeds Fleckvieh (dual purpose Simmental), Holstein Friesian and Brown Swiss, were used. Test-day records within 21 days before and 21 days after a mastitis diagnosis were defined as mastitis cases. Three different models (MIR, SCS, MIR + SCS) were compared, applying Partial Least Squares Discriminant Analysis. Results of external validation in the overall time window (−/+21 days) showed area under receiver operating characteristic curves (AUC) of 0.70 when based only on MIR, 0.72 when based only on SCS, and 0.76 when based on both. Considering as mastitis cases only the test-day records within 7 days after mastitis diagnosis, the corresponding areas under the curve were 0.77, 0.83 and 0.85. Hence, the model combining MIR spectral data and SCS was performing best. Mastitis probabilities derived from the prediction models are potentially valuable for routine mastitis monitoring for farmers, as well as for the genetic evaluation of the trait udder health.
Effectiveness of Intramammary Antibiotics, Internal Teat Sealants, or Both at Dry-Off in Dairy Cows: Clinical Mastitis and Culling Outcomes
Authors: Aly Sharif S. , Okello Emmanuel , ElAshmawy Wagdy R. , Williams Deniece R. , Anderson Randall J. , Rossitto Paul , Tonooka Karen , Glenn Kathy , Karle Betsy , Lehenbauer Terry W.
Published: July 2022

Intramammary antibiotic (AB) and internal teat sealants (TS) infusion at dry-off have been used to prevent intramammary infections (IMI) in dairy cows during the dry period and reduce the risk of mastitis during the dry period and subsequent lactation. A randomized clinal trial was completed on eight California dairy herds to estimate the effects of different dry cow therapies (AB, TS, AB + TS or None) on clinical mastitis and culling. A total of 1273 cows were randomized to one of the four treatment groups over summer and winter seasons. For each enrolled cow, microbiological testing was done on quarter milk samples collected from the first detection of clinical mastitis within the first 150 days in milk (DIM) in the subsequent lactation. Statistical analysis was done using generalized linear mixed models. There were no significant differences in the odds of clinical mastitis or culling between cows treated with AB, TS, or AB + TS compared to the controls. Dry cow therapy with AB and/or TS had no statistically significant effect on clinical mastitis and cow culling during the first 150 DIM.
Pharmacokinetics and Safety of Doripenem in Healthy Chinese Subjects and Monte Carlo Dosing Simulations
Authors: Wang Yu , Liu Xiaofen , Li Kun , Fan Yaxin , Yu Jicheng , Wu Hailan , Li Yi , Wu Xiaojie , Guo Beining , Li Xin , Hu Jiali , Wu Jufang , Cao Guoying , Zhang Jing
Published: July 2022

The aim of this study was to investigate the pharmacokinetics (PK) of doripenem in healthy Chinese subjects and evaluate the optimal dosage regimens of doripenem. A randomized, single-dose, three-period, self-crossover controlled extended-infusion clinical trial was conducted with 12 healthy Chinese subjects. Plasma and urine samples were collected to determine doripenem concentrations. Non-compartmental and population PK analysis were performed to characterize the PK of doripenem. The Monte Carlo simulation was employed to optimize dosing regimens based on the probability of target attainment of doripenem against pathogens with different minimum inhibitory concentrations (MIC). All 12 healthy Chinese subjects completed the study, and the doripenem was well tolerated. The study showed linearity relationships in the peak plasma concentration and the area under the concentration-time curve after intravenous infusion of doripenem from 0.25 g to 1.0 g. The cumulative urinary recovery rate of doripenem was 68.1–72.0% within 24 h. PPK modeling showed a two-compartmental model, with first-order elimination presenting the best fit for doripenem PK. Monte Carlo simulation results showed that 1.0 g q12h or 0.5 g q8h was an optimal regimen for pathogens susceptible to doripenem (MIC ≤ 1 mg/L); while high dose and extended infusion (1 g, q8h, 4 h infusion) was proposed for unsusceptible pathogens (2 ≤ MIC ≤ 8 mg/L). In the dose range of 0.25 to 1.0 g, doripenem showed linear pharmacokinetics. Doripenem at 1.0 g with a prolonged infusion time of 4 h was predicted to be effective against pathogens with MICs as high as 8 mg/L.
Immunogenicity of Endolysin PlyC
Authors: Harhala Marek Adam , Gembara Katarzyna , Nelson Daniel C. , Miernikiewicz Paulina , Dąbrowska Krystyna
Published: July 2022

Endolysins are bacteriolytic enzymes derived from bacteriophages. They represent an alternative to antibiotics, since they are not susceptible to conventional antimicrobial resistance mechanisms. Since non-human proteins are efficient inducers of specific immune responses, including the IgG response or the development of an allergic response mediated by IgE, we evaluated the general immunogenicity of the highly active antibacterial enzyme, PlyC, in a human population and in a mouse model. The study includes the identification of molecular epitopes of PlyC. The overall assessment of potential hypersensitivity to this protein and PlyC-specific IgE testing was also conducted in mice. PlyC induced efficient IgG production in mice, and the molecular analysis revealed that PlyC-specific IgG interacted with four immunogenic regions identified within the PlyCA subunit. In humans, approximately 10% of the population demonstrated IgG reactivity to the PlyCB subunit only, which is attributed to cross-reactions since this was a naïve serum. Of note, in spite of being immunogenic, PlyC induced a normal immune response, without hypersensitivity, since both the animals challenged with PlyC and in the human population PlyC-specific IgE was not detected.