bluuebunny/crossref_metadata_2025 huggingface dataset:
Mapping of a Myosin-binding Domain and a Regulatory Phosphorylation Site in M-Protein, a Structural Protein of the Sarcomeric M Band
Authors: Obermann Wolfgang M.J. , van der Ven Peter F.M. , Steiner Frank , Weber Klaus , Fürst Dieter O.
Published: July 2013

The myofibrils of cross-striated muscle fibers contain in their M bands cytoskeletal proteins whose main function seems to be the stabilization of the three-dimensional arrangement of thick filaments. We identified two immunoglobin domains (Mp2–Mp3) of M-protein as a site binding to the central region of light meromyosin. This binding is regulated in vitro by phosphorylation of a single serine residue (Ser76) in the immediately adjacent amino-terminal domain Mp1. M-protein phosphorylation by cAMP-dependent kinase A inhibits binding to myosin LMM. Transient transfection studies of cultured cells revealed that the myosin-binding site seems involved in the targeting of M-protein to its location in the myofibril. Using the same method, a second myofibril-binding site was uncovered in domains Mp9–Mp13. These results support the view that specific phosphorylation events could be also important for the control of sarcomeric M band formation and remodeling.
Fascin, an Actin-bundling Protein, Induces Membrane Protrusions and Increases Cell Motility of Epithelial Cells
Authors: Yamashiro Shigeko , Yamakita Yoshihiko , Ono Shoichiro , Matsumura Fumio
Published: July 2013

Fascin is an actin-bundling protein that is found in membrane ruffles, microspikes, and stress fibers. The expression of fascin is greatly increased in many transformed cells, as well as in specialized normal cells including neuronal cells and antigen-presenting dendritic cells. A morphological characteristic common to these cells expressing high levels of fascin is the development of many membrane protrusions in which fascin is predominantly present. To examine whether fascin contributes to the alterations in microfilament organization at the cell periphery, we have expressed fascin in LLC-PK1 epithelial cells to levels as high as those found in transformed cells and in specialized normal cells. Expression of fascin results in large changes in morphology, the actin cytoskeleton, and cell motility: fascin-transfected cells form an increased number of longer and thicker microvilli on apical surfaces, extend lamellipodia-like structures at basolateral surfaces, and show disorganization of cell–cell contacts. Cell migration activity is increased by 8–17 times when assayed by modified Boyden chamber. Microinjection of a fascin protein into LLC-PK1 cells causes similar morphological alterations including the induction of lamellipodia at basolateral surfaces and formation of an increased number of microvilli on apical surfaces. Furthermore, microinjection of fascin into REF-52 cells, normal fibroblasts, induces the formation of many lamellipodia at all regions of cell periphery. These results together suggest that fascin is directly responsible for membrane protrusions through reorganization of the microfilament cytoskeleton at the cell periphery.
Distinct Morphological Phenotypes of Cell Fusion Mutants
Authors: Gammie Alison E. , Brizzio Valeria , Rose Mark D.
Published: July 2013

Cell fusion in yeast is the process by which two haploid cells fuse to form a diploid zygote. To dissect the pathway of cell fusion, we phenotypically and genetically characterized four cell fusion mutants,fus6/spa2, fus7/rvs161, fus1, and fus2. First, we examined the complete array of single and double mutants. In all cases but one, double mutants exhibited stronger cell fusion defects than single mutants. The exception was rvs161Δfus2Δ, suggesting that Rvs161p and Fus2p act in concert. Dosage suppression analysis showed that Fus1p and Fus2p act downstream or parallel to Rvs161p and Spa2p. Second, electron microscopic analysis was used to define the mutant defects in cell fusion. In wild-type prezygotes vesicles were aligned and clustered across the cell fusion zone. The vesicles were associated with regions of cell wall thinning. Analysis of Fus−zygotes indicated that Fus1p was required for the normal localization of the vesicles to the zone of cell fusion, and Spa2p facilitated their clustering. In contrast, Fus2p and Rvs161p appeared to act after vesicle positioning. These findings lead us to propose that cell fusion is mediated in part by the localized release of vesicles containing components essential for cell fusion.
Cleavage of β-Catenin and Plakoglobin and Shedding of VE-Cadherin during Endothelial Apoptosis: Evidence for a Role for Caspases and Metalloproteinases
Authors: Herren Barbara , Levkau Bodo , Raines Elaine W. , Ross Russell
Published: July 2013

Growth factor deprivation of endothelial cells induces apoptosis, which is characterized by membrane blebbing, cell rounding, and subsequent loss of cell–matrix and cell–cell contacts. In this study, we show that initiation of endothelial apoptosis correlates with cleavage and disassembly of intracellular and extracellular components of adherens junctions. β-Catenin and plakoglobin, which form intracellular links between vascular endothelial cadherin (VE-cadherin) and actin-binding α-catenin in adherens junctions, are cleaved in apoptotic cells. In vitro incubations of cell lysates and immunoprecipitates with recombinant caspases indicate that CPP32 and Mch2 are involved, possibly by initiating proteolytic processing. Cleaved β-catenin from lysates of apoptotic cells does not bind to endogenous α-catenin, whereas plakoglobin retains its binding capacity. The extracellular portion of the adherens junctions is also altered during apoptosis because VE-cadherin, which mediates endothelial cell–cell interactions, dramatically decreases on the surface of cells. An extracellular fragment of VE-cadherin can be detected in the conditioned medium, and this “shedding” of VE-cadherin can be blocked by an inhibitor of metalloproteinases. Thus, cleavage of β-catenin and plakoglobin and shedding of VE-cadherin may act in concert to disrupt structural and signaling properties of adherens junctions and may actively interrupt extracellular signals required for endothelial cell survival.
Formation and Turnover of NSF- and SNAP-containing “Fusion” Complexes Occur on Undocked, Clathrin-coated Vesicle–derived Membranes
Authors: Swanton Eileithyia , Sheehan John , Bishop Naomi , High Stephen , Woodman Philip
Published: July 2013

Specificity of vesicular transport is determined by pair-wise interaction between receptors (SNAP receptors or SNAREs) associated with a transport vesicle and its target membrane. Two additional factors, N-ethylmaleimide-sensitive fusion protein (NSF) and soluble NSF attachment protein (SNAP) are ubiquitous components of vesicular transport pathways. However, the precise role they play is not known. On the basis that NSF and SNAP can be recruited to preformed SNARE complexes, it has been proposed that NSF- and SNAP-containing complexes are formed after SNARE-dependent docking of transport vesicles. This would enable ATPase-dependent complex disassembly to be coupled directly to membrane fusion. Alternatively, binding and release of NSF/SNAP may occur before vesicle docking, and perhaps be involved in the activation of SNAREs. To gain more information about the point at which so-called 20S complexes form during the transport vesicle cycle, we have examined NSF/SNAP/SNARE complex turnover on clathrin-coated vesicle–derived membranes in situ. This has been achieved under conditions in which the extent of membrane docking can be precisely monitored. We demonstrate by UV-dependent cross-linking experiments, coupled to laser light-scattering analysis of membranes, that complexes containing NSF, SNAP, and SNAREs will form and dissociate on the surface of undocked transport vesicles.
Serine and Threonine Phosphorylation of the Paxillin LIM Domains Regulates Paxillin Focal Adhesion Localization and Cell Adhesion to Fibronectin
Authors: Brown Michael C. , Perrotta Joseph A. , Turner Christopher E.
Published: July 2013

We have previously shown that the LIM domains of paxillin operate as the focal adhesion (FA)-targeting motif of this protein. In the current study, we have identified the capacity of paxillin LIM2 and LIM3 to serve as binding sites for, and substrates of serine/threonine kinases. The activities of the LIM2- and LIM3-associated kinases were stimulated after adhesion of CHO.K1 cells to fibronectin; consequently, a role for LIM domain phosphorylation in regulating the subcellular localization of paxillin after adhesion to fibronectin was investigated. An avian paxillin-CHO.K1 model system was used to explore the role of paxillin phosphorylation in paxillin localization to FAs. We found that mutations of paxillin that mimicked LIM domain phosphorylation accelerated fibronectin-induced localization of paxillin to focal contacts. Further, blocking phosphorylation of the LIM domains reduced cell adhesion to fibronectin, whereas constitutive LIM domain phosphorylation significantly increased the capacity of cells to adhere to fibronectin. The potentiation of FA targeting and cell adhesion to fibronectin was specific to LIM domain phosphorylation as mutation of the amino-terminal tyrosine and serine residues of paxillin that are phosphorylated in response to fibronectin adhesion had no effect on the rate of FA localization or cell adhesion. This represents the first demonstration of the regulation of protein localization through LIM domain phosphorylation and suggests a novel mechanism of regulating LIM domain function. Additionally, these results provide the first evidence that paxillin contributes to “inside-out” integrin-mediated signal transduction.
Na–H Exchange Acts Downstream of RhoA to Regulate Integrin-induced Cell Adhesion and Spreading
Authors: Tominaga Tomoko , Barber Diane L.
Published: July 2013

The ubiquitously expressed Na–H exchanger NHE1 functions in regulating intracellular pH and cell volume. NHE1 activity is stimulated by hormones, growth factors, and activation of integrin receptors. We recently determined that NHE1 activity is also stimulated by activation of the low molecular weight GTPase RhoA and that increases in NHE1 activity are necessary for RhoA-induced formation of actin stress fibers. We now show that NHE1 acts downstream of RhoA to modulate initial steps in integrin signaling for the assembly of focal adhesions. Adhesion of CCL39 fibroblasts on fibronectin was markedly delayed in the presence of the NHE inhibitor ethylisopropylamiloride. In mutant PS120 cells, derived from CCL39 fibroblasts but lacking NHE1, adhesion was also delayed but was rescued in PS120 cells stably expressing NHE1. In the absence of NHE1 activity, cell spreading was inhibited, and the accumulation of integrins, paxillin, and vinculin at focal contacts was impaired. Additionally, tyrosine phosphorylation of p125FAKinduced by integrin clustering was also impaired. Inactivation of RhoA with C3 transferase and inhibition of the Rho-kinase p160ROCK with the pyridine derivative Y-27632 completely abolished activation of NHE1 by integrins but not by platelet-derived growth factor. These findings indicate that NHE1 acts downstream of RhoA to contribute a previously unrecognized critical signal to proximal events in integrin-induced cytoskeletal reorganization.
An α-Tubulin Mutant Destabilizes the Heterodimer: Phenotypic Consequences and Interactions with Tubulin-binding Proteins
Authors: Vega Leticia R. , Fleming James , Solomon Frank
Published: July 2013

Many effectors of microtubule assembly in vitro enhance the polymerization of subunits. However, several Saccharomyces cerevisiae genes that affect cellular microtubule-dependent processes appear to act at other steps in assembly and to affect polymerization only indirectly. Here we use a mutant α-tubulin to probe cellular regulation of microtubule assembly.tub1-724 mutant cells arrest at low temperature with no assembled microtubules. The results of several assays reported here demonstrate that the heterodimer formed between Tub1-724p and β-tubulin is less stable than wild-type heterodimer. The unstable heterodimer explains several conditional phenotypes conferred by the mutation. These include the lethality of tub1-724haploid cells when the β-tubulin–binding protein Rbl2p is either overexpressed or absent. It also explains why theTUB1/tub1-724 heterozygotes are cold sensitive for growth and why overexpression of Rbl2p rescues that conditional lethality. Both haploid and heterozygous tub1-724 cells are inviable when another microtubule effector, PAC2, is overexpressed. These effects are explained by the ability of Pac2p to bind α-tubulin, a complex we demonstrate directly. The results suggest that tubulin-binding proteins can participate in equilibria between the heterodimer and its components.
Binding of Insulin-like Growth Factor (IGF)–Binding Protein-5 to Smooth-Muscle Cell Extracellular Matrix Is a Major Determinant of the Cellular Response to IGF-I
Authors: Parker Alex , Rees Catherine , Clarke Jane , Busby Walker H. , Clemmons David R.
Published: July 2013

Insulin-like growth factor–binding protein-5 (IGFBP-5) has been shown to bind to fibroblast extracellular matrix (ECM). Extracellular matrix binding of IGFBP-5 leads to a decrease in its affinity for insulin-like growth factor-I (IGF-I), which allows IGF-I to better equilibrate with IGF receptors. When the amount of IGFBP-5 that is bound to ECM is increased by exogenous addition, IGF-I’s effect on fibroblast growth is enhanced. In this study we identified the specific basic residues in IGFBP-5 that mediate its binding to porcine smooth-muscle cell (pSMC) ECM. An IGFBP-5 mutant containing alterations of basic residues at positions 211, 214, 217, and 218 had the greatest reduction in ECM binding, although three other mutants, R214A, R207A/K211N, and K202A/R206N/R207A, also had major decreases. In contrast, three other mutants, R201A/K202N/R206N/R208A, and K217N/R218A and K211N, had only minimal reductions in ECM binding. This suggested that residues R207 and R214 were the most important for binding, whereas alterations in K211 and R218, which align near them, had minimal effects. To determine the effect of a reduction in ECM binding on the cellular replication response to IGF-I, pSMCs were transfected with the mutant cDNAs that encoded the forms of IGFBPs with the greatest changes in ECM binding. The ECM content of IGFBP-5 from cultures expressing the K211N, R214A, R217A/R218A, and K202A/R206N/R207A mutants was reduced by 79.6 and 71.7%, respectively, compared with cells expressing the wild-type protein. In contrast, abundance of the R201A/K202N/R206N/R208A mutant was reduced by only 14%. Cells expressing the two mutants with reduced ECM binding had decreased DNA synthesis responses to IGF-I, but the cells expressing the R201A/K202N/R206N/R208A mutant responded well to IGF-I. The findings suggest that specific basic amino acids at positions 207 and 214 mediate the binding of IGFBP-5 to pSMC/ECM. Smooth-muscle cells that constitutively express the mutants that bind weakly to ECM are less responsive to IGF-I, suggesting that ECM binding of IGFBP-5 is an important variable that determines cellular responsiveness.
Phosphorylation of Sic1, a Cyclin-dependent Kinase (Cdk) Inhibitor, by Cdk Including Pho85 Kinase Is Required for Its Prompt Degradation
Authors: Nishizawa Masafumi , Kawasumi Masaoki , Fujino Marie , Toh-e Akio
Published: July 2013

In the yeast Saccharomyces cerevisiae, Sic1, an inhibitor of Clb-Cdc28 kinases, must be phosphorylated and degraded in G1for cells to initiate DNA replication, and Cln-Cdc28 kinase appears to be primarily responsible for phosphorylation of Sic1. The Pho85 kinase is a yeast cyclin-dependent kinase (Cdk), which is not essential for cell growth unless both CLN1 andCLN2 are absent. We demonstrate that Pho85, when complexed with Pcl1, a G1cyclin homologue, can phosphorylate Sic1 in vitro, and that Sic1 appears to be more stable inpho85Δ cells. Three consensus Cdk phosphorylation sites present in Sic1 are phosphorylated in vivo, and two of them are required for prompt degradation of the inhibitor. Pho85 and other G1Cdks appear to phosphorylate Sic1 at different sites in vivo. Thus at least two distinct Cdks can participate in phosphorylation of Sic1 and may therefore regulate progression through G1.
The Dynamics of Golgi Protein Traffic Visualized in Living Yeast Cells
Authors: Wooding Steven , Pelham Hugh R.B.
Published: July 2013

We describe for the first time the visualization of Golgi membranes in living yeast cells, using green fluorescent protein (GFP) chimeras. Late and early Golgi markers are present in distinct sets of scattered, moving cisternae. The immediate effects of temperature-sensitive mutations on the distribution of these markers give clues to the transport processes occurring. We show that the late Golgi marker GFP-Sft2p and the glycosyltransferases, Anp1p and Mnn1p, disperse into vesicle-like structures within minutes of a temperature shift insec18, sft1, and sed5cells, but not in sec14 cells. This is consistent with retrograde vesicular traffic, mediated by the vesicle SNARE Sft1p, to early cisternae containing the target SNARE Sed5p. Strikingly, Sed5p itself moves rapidly to the endoplasmic reticulum (ER) insec12 cells, implying that it cycles through the ER. Electron microscopy shows that Golgi membranes vesiculate insec18 cells within 10 min of a temperature shift. These results emphasize the dynamic nature of Golgi cisternae and satisfy the kinetic requirements of a cisternal maturation model in which all resident proteins must undergo retrograde vesicular transport, either within the Golgi complex or from there to the ER, as anterograde cargo advances.
Neighbors and Extension Agents in Ethiopia: Who Matters More for Technology Adoption?
Authors: Krishnan Pramila , Patnam Manasa
Published: July 2013

The increased adoption of fertilizer and improved seeds are two key aspects to raising the level of land productivity in Ethiopian agriculture. However, the adoption and diffusion of such technologies has been slow. We use data from Ethiopia between 1999–2009 to examine the role of learning from extension agents versus learning from neighbors for both improved seeds and fertilizer adoption. We combine farmers' spatial networks with panel data to identify these influences, and find that while the initial impact of extension agents was high, the effect wore off after some time, in contrast to learning from neighbors.
Positive End-expiratory Pressure Influences Echocardiographic Measures of Diastolic Function
Authors: Juhl-Olsen Peter , Hermansen Johan Fridolf , Frederiksen Christian Alcaraz , Rasmussen Linda Aagaard , Jakobsen Carl-Johan , Sloth Erik
Published: July 2013

Background:

Ultrasonography of the cardiovascular system is pivotal for hemodynamic assessment. Diastolic function is evaluated with a combination of tissue Doppler (e’ and a’) and pulsed Doppler (E and A) measures of transmitral- and mitral valve annuli velocities. However, accurate echocardiographic evaluation in the intensive care unit or perioperative setting is contingent on relative resistance to positive pressure ventilation and changes in preload. This study aimed to evaluate the effects of positive end-expiratory pressure (PEEP) and positioning on echocardiographic measures of diastolic function.

Methods:

The study was a prospective, randomized, crossover study. Cardiac surgery patients with ejection fraction greater than 45% and averaged e’ of 9 or more were included. Postoperatively, anesthetized patients were randomized into six combinations of PEEP (0, 6, 12 cm H2O) and positions (horizontal, Trendelenburg). At each combination, e’ (primary endpoint), a’, E, and A were obtained with transesophageal echocardiography along with left ventricular area. Image analysis was performed blinded to the protocol.

Results:

Thirty patients completed the study. PEEP decreased lateral e’ from 6.6 ± 3.6 to 5.3 ± 3.0 cm/s (P < 0.001) in the horizontal position and from 7.4 ± 4.2 to 6.5 ± 3.3 cm/s (P < 0.001) in Trendelenburg. Similar results were found for septal e’, a’ bilaterally and transmitral pulsed Doppler measures, and PEEP decreased left ventricular area. E/A, E/e’, and e’/a’ remained unaffected by PEEP and positioning.

Conclusions:

When evaluating diastolic function by echocardiography, the levels of PEEP and its effect on ventricular area have to be taken into account. In addition, this study dissuades the use of E/e’ for tracking changes in left ventricular filling pressures in cardiac surgery patients.
Polymer solar cells
Authors: Greenham Neil C.
Published: July 2013

This article reviews the motivations for developing polymer-based photovoltaics and describes some of the material systems used. Current challenges are identified, and some recent developments in the field are outlined. In particular, recent work to image and control nanostructure in polymer-based solar cells is reviewed, and very recent progress is described using the unique properties of organic semiconductors to develop strategies that may allow the Shockley–Queisser limit to be broken in a simple photovoltaic cell.
Concentrating solar thermal power
Authors: Müller-Steinhagen Hans
Published: July 2013

In addition to wind and photovoltaic power, concentrating solar thermal power (CSP) will make a major contribution to electricity provision from renewable energies. Drawing on almost 30 years of operational experience in the multi-megawatt range, CSP is now a proven technology with a reliable cost and performance record. In conjunction with thermal energy storage, electricity can be provided according to demand. To date, solar thermal power plants with a total capacity of 1.3 GW are in operation worldwide, with an additional 2.3 GW under construction and 31.7 GW in advanced planning stage. Depending on the concentration factors, temperatures up to 1000
°
C can be reached to produce saturated or superheated steam for steam turbine cycles or compressed hot gas for gas turbine cycles. The heat rejected from these thermodynamic cycles can be used for sea water desalination, process heat and centralized provision of chilled water. While electricity generation from CSP plants is still more expensive than from wind turbines or photovoltaic panels, its independence from fluctuations and daily variation of wind speed and solar radiation provides it with a higher value. To become competitive with mid-load electricity from conventional power plants within the next 10–15 years, mass production of components, increased plant size and planning/operating experience will be accompanied by technological innovations. On 30 October 2009, a number of major industrial companies joined forces to establish the so-called DESERTEC Industry Initiative, which aims at providing by 2050 15 per cent of European electricity from renewable energy sources in North Africa, while at the same time securing energy, water, income and employment for this region. Solar thermal power plants are in the heart of this concept.
Using a standard sample to estimate the X-ray wavelength of the 1W2A SAXS beamline at BSRF
Authors: Liu Jun , Li Zhihong
Published: July 2013

This contribution describes a method for measuring diffraction peaks of a standard sample to estimate the incident X-ray wavelength at the 1W2A SAXS beamline at BSRF. A simple simulation has been performed to establish the factors influencing the accuracy of the wavelength measurement. Appropriate measurement conditions and error control measures are presented. An actual experimental example further verifies the effectiveness of the simulation. This method is particularly suitable for synchrotron radiation beamlines using bent triangular crystal monochromators.
An efficient numerical tool for dose deposition prediction applied to synchrotron medical imaging and radiation therapy
Authors: Mittone Alberto , Baldacci Fabien , Bravin Alberto , Brun Emmanuel , Delaire François , Ferrero Claudio , Gasilov Sergei , Freud Nicolas , Létang Jean Michel , Sarrut David , Smekens François , Coan Paola
Published: July 2013

Medical imaging and radiation therapy are widely used synchrotron-based techniques which have one thing in common: a significant dose delivery to typically biological samples. Among the ways to provide the experimenters with image guidance techniques indicating optimization strategies, Monte Carlo simulation has become the gold standard for accurately predicting radiation dose levels under specific irradiation conditions. A highly important hampering factor of this method is, however, its slow statistical convergence. A track length estimator (TLE) module has been coded and implemented for the first time in the open-source Monte Carlo codeGATE/Geant4. Results obtained with the module and the procedures used to validate them are presented. A database of energy-absorption coefficients was also generated, which is used by the TLE calculations and is now also included inGATE/Geant4. The validation was carried out by comparing the TLE-simulated doses with experimental data in a synchrotron radiation computed tomography experiment. The TLE technique shows good agreementversusboth experimental measurements and the results of a classical Monte Carlo simulation. Compared with the latter, it is possible to reach a pre-defined statistical uncertainty in about two to three orders of magnitude less time for complex geometries without loss of accuracy.
Real exchange rate misalignment and economic growth in Malaysia
Authors: Tsen Wong Hock
Published: July 2013

Purpose

The purpose of this study is to examine real exchange rate misalignment and economic growth in Malaysia.

Design/methodology/approach

The result of the autoregressive distributed lag (ARDL) approach and the generalized forecast error variance decomposition.

Findings

The result of the ARDL approach shows an increase in the real interest rate differential, productivity differential, the real oil price or reserve differential will lead to an appreciation of the real exchange rate in the long run. The result of the generalized forecast error variance decomposition shows that the real interest rate differential, productivity differential, the real oil price, and reserve differential are generally important to the real exchange rate determination. Moreover, the result of the ARDL approach shows that an increase in real exchange rate misalignment will lead to a decrease in economic growth. More specifically, devaluation will promote economic growth and appreciation will hurt economic growth. Exchange rate can be a policy variable to influence economic growth. Real exchange rate misalignment should be avoided to enable the allocation of resources in the economy according to fundamentals.

Originality/value

A managed floating exchange rate regime could be a choice of exchange rate regime in other developing countries to achieve rapid economic growth.
Collaborative design: outdoor environments for veterans with PTSD
Authors: Wagenfeld Amy , Roy‐Fisher Connie , Mitchell Carolyn
Published: July 2013

Purpose

Providing veterans diagnosed with post‐traumatic stress disorder (PTSD), their families, and staff opportunities to experience physical and mental restoration in outdoor environments designed based on evidence is important. The purpose of this paper is to explore the relationship between evidence‐based collaborative design of outdoor environments and their potential capacity to contribute to a veteran's journey to wellness.

Design/methodology/approach

There is no existing precedent in the peer‐reviewed literature linking positive health outcomes associated with outdoor environments to veterans with PTSD. This review of the literature is conceptualized as a means to extrapolate these benefits to this unique population.

Findings

Access to nature improves physiological and psychological health outcomes. A collaborative design approach ensures that design outcomes meet specific populations' needs.

Practical implications

Many service‐members are reluctant to seek traditional treatments for PTSD, fearing threat to future military service and limited available resources. Alternative treatments, access to sensitively designed outdoor environments and/or a re‐examination of traditional treatments and the environments in which they are provided supports best practice approaches to ameliorating the debilitating effects of this disorder.

Social implications

An integrated design approach blending the skills of landscape architecture and occupational therapy is key to achieve design outcomes that support the healing process to meet the needs of this vulnerable population.

Originality/value

An inter and/or trans‐disciplinary team approach to design and programming of outdoor environments for veterans with PTSD blends landscape architecture with occupational therapy to ensure both form and function are achieved, thus positing positive health outcomes.
Retrocomputing as preservation and remix
Authors: Takhteyev Yuri , DuPont Quinn
Published: July 2013

Purpose

The paper's aim is to describe the world of retrocomputing, a constellation of largely non‐professional practices involving old computing technology. It seeks to show how retrocomputing serves the goals of collection and preservation, particularly in regards to historic software, and how retrocomputing practices challenge traditional notions of authenticity. It then seeks to propose an alternative conceptualization and suggest new avenues for collaboration between retrocomputing practitioners and memory institutions.

Design/methodology/approach

The paper is based on extensive observation of retrocomputing projects, conducted primarily online.

Findings

Retrocomputing includes many activities that can be seen as constituting collection and preservation. At the same time, it is often transformative, producing assemblages that “remix” fragments from the past with newer elements or joining together historic components that were never combined before. While such “remix” may seem to undermine preservation, it also allows for fragments of computing history to be reintegrated into a living, ongoing practice, contributing to preservation in a broader sense. The seemingly unorganized nature of retrocomputing assemblages also provides space for alternative “situated knowledges” and histories of computing, which can sometimes be quite sophisticated.

Research limitations/implications

Retrocomputing challenges established notions of collection and preservation. A “situated knowledges” perspective provides a possible resolution.

Practical implications

Retrocomputing presents memory institutions (and libraries in particular) with an opportunity for new forms of collaboration in collection and preservation of software applications.

Originality/value

The paper puts at the center the ways in which retrocomputing challenges the established notions of collection and preservation. It offers alternative conceptualizations that suggest new forms of collaboration.
Beyond loyalty: customer satisfaction, loyalty, and fortitude
Authors: Fraering Martin , S. Minor Michael
Published: July 2013

Purpose

– This paper aims to discuss the first effort to examine the relationships between satisfaction, the four loyalty phases, fortitude, and a sense of virtual community.

Design/methodology/approach

– Oliver proposed an innovative framework to explain the relationships between satisfaction, loyalty, fortitude, and a sense of community.

Findings

– Analysis of questionnaire responses of 493 customers of banks and credit unions indicated that satisfaction, cognitive, affective, conative, and action loyalty are positively related to fortitude.

Research limitations/implications

– The Beyond Loyalty Model (BLM) does not address important strategic issues often associated with loyalty, such as firm profitability, complaint resolution, and firm profitability.

Practical implications

– This research is the first to find that customers of financial institutions acquire satisfaction and strong loyalty ties with their bank or credit union after dealing with their financial services provider for a relatively short period of time. Thus financial institutions should consistently seek relationship-building opportunities from the outset of their relationships with their customers.

Originality/value

– The resulting Beyond Loyalty Model (BLM) improves upon the American Bankers Association
'
s ABA Financial Client Satisfaction Index, and is a means by which financial institutions can monitor and enhance the satisfaction, loyalty, and fortitude of the customers of financial institutions. Further, the increasing acceptance of virtual banking calls for additional study of this area.
Carbon emission reduction strategies in the UK industrial sectors: an empirical study
Authors: Renukappa Suresh , Akintoye Akintola , Egbu Charles , Goulding Jack
Published: July 2013

Purpose

The problem of climate change is one aspect of the broader problem of sustainability. Many businesses in most sectors now accept that they must address the issue of climate change in order to survive and grow in ever‐changing entangled business economies. Due to mounting pressure from stakeholders, top executives of many organisations are now implementing various carbon emissions reduction strategies. However, the extent to which businesses embrace climate change and carbon management as an integral pillar of their business models remains unclear and poorly understood. This paper seeks to address these issues.

Design/methodology/approach

The aim of this research is to investigate the key carbon emissions reduction initiatives currently being implemented in the UK industrial sectors so as to improve their competitiveness. In order to achieve this aim, a mixed research methodological approach was adopted to collect and analyse data. Four industry sectors were examined, specifically: energy and utilities, transportation, construction and not‐for‐profit organisations; with specific respect to their environmental, social and economic impact on the UK society.

Findings

The level of implementation of carbon emissions reduction strategies within the UK industrial sectors is fairly “low” and varies significantly across the four sectors; with relatively high uptake in the energy and utilities sector, and low uptake in the construction sector. The level of implementation of change management initiatives to deal with carbon emissions reduction initiatives is also relatively “low”.

Practical implications

This study suggests that carbon emissions reduction strategies are in their infancy. Taken together, the impact of management commitment and leadership, climate change‐related policies, structures, reward systems, training programmes and performance reporting are key factors in successful implementation of low carbon strategies. The paper concludes that there is a need for cross‐sector collaboration to capture and share best and worst practices relating to low carbon strategies.

Originality/value

The paper provides a richer insight into the understanding and awareness of low carbon strategies for competitive advantage.
Eve's emancipation or lingering subordination to Adam?
Authors: Aloni Gil , Syna Desivilya Helena
Published: July 2013

Purpose

The current study aims to examine couples' conjoint negotiation with a third party, testing the effects of asymmetrical contextual ambiguity, gender stereotypes' priming and egalitarianism. It predicted differences in the processes of decision making between egalitarian and traditional couples, reflected in choices of female or male negotiator.

Design/methodology/approach

Egalitarianism levels were measured by the Altrocchi and Crosby Marriage Questionnaire. The asymmetrical contextual ambiguity was manipulated through two newly constructed negotiation cases – one feminine‐stereotyped and the other masculine‐stereotyped, based on Miles and LaSalle. Priming of gender stereotypes was manipulated using two passages inducing explicit or implicit priming, based on Kray, Galinsky and Thompson. Primary statistical analysis was χ2 test for equal proportions.

Findings

The hypotheses were by and large supported: as expected in all four experimental conditions, traditional couples chose men as their negotiator. By contrast, egalitarian couples tended to nominate their negotiator depending on the situation (feminine, masculine, and under implicit priming). In addition, under explicit priming their selection was in the predicted direction but not significant.

Practical implications

This study provides insights with respect to effective ways to conduct conjoint negotiations. In addition, it indicates the need to enhance women's negotiation self‐efficacy, so that they can become more active in negotiation processes.

Originality/value

The current study explored real‐life couples' conjoint negotiation with a third party, rather than examining couples' internal negotiation processes or individuals' dyadic negotiation, which prevailed in extant research. Future research should adopt the focus on genuine couples' conjoint negotiation, employed in this study.
Collective narratives as indicators of examining intergroup relations
Authors: Srour Anan , Sagy Shifra , Mana Adi , Mjally‐Knani Serene
Published: July 2013

Purpose

This study aims to examine inter‐group relations between two religious minorities, Palestinian Christians and Muslim citizens of Israel, by measuring perceptions of in‐group and “other” group collective narratives.

Design/methodology/approach

Data were collected from a representative sample of 1,121 Muslims and 756 Christians in Israel. The questionnaire presents narratives that are common among Muslim and Christian populations in Israel, relating to eight different historical, social, or political events. Subjects were asked to rate levels of legitimacy, feeling of empathy, and anger towards each one of the presented narratives.

Findings

In comparison to Muslims, Christians reported a lower tendency to give legitimacy to the narrative of the other, showed more emotions of anger, and lower feeling of empathy towards the Muslim narrative. In addition, a content analysis of the narratives that were used in the questionnaire revealed two different patterns of response to narratives. One related to narratives with national issues, where Christians seemed to be excluded from the Palestinian nation, and the other related to narratives with religious issues, where Muslims reported more anger, less empathy and less legitimacy towards the Christian narratives.

Originality/value

The paper presents a new tool based on Sagy, Adwan and Kaplan, developed to examine perceptions of in‐group and “other” group collective narratives. This study examined the tool in a different context of conflict. In addition to the quantitative measures of the perceptions of the collective narratives, the paper describes a new method for analyzing the data collected by this tool.
Urban poverty in Vietnam: determinants and policy implications
Authors: Nguyen Cuong , Linh Vu , Nguyen Thang
Published: July 2013

Purpose

The objective of the paper is to examine the profile and determinants of urban poverty in the two largest cities in Vietnam – Hanoi and Ho Chi Minh. The paper also investigates the dynamic aspect of urban poverty in Vietnam.

Design/methodology/approach

The authors use regression and data from the 2009 Urban Poverty Survey to examine the determinants of poverty in Vietnam. To analyse the poverty dynamics, an approach by Carter and May is used to decompose poverty into structural and stochastic poverty.

Findings

Using the poverty line of 12,000 thousand VND/person/year, the poverty incidence is estimated at 17.4 percent for Hanoi and 12.5 percent for Ho Chi Minh (HCM) City. There is a large proportion of the poor who are found stochastically poor. Hanoi has higher rates of structural poverty than HCM City. The proportion of structurally poor and stochastically non‐poor is rather small. Overall, the poor have fewer assets than the non‐poor. The poor also have poorer housing conditions, especially substantially lower access to tap water than the non‐poor. Heads of the poor households tend to have lower education and unskilled work than the heads of the non‐poor households.

Originality/value

In cities of Vietnam, a large proportion of the poor are found stochastically poor.
Innovation systems in motion: an early music case
Authors: Castro‐Martínez Elena , Recasens Albert , Jiménez‐Sáez Fernando
Published: July 2013

Purpose

This study aims to provide an in‐depth understanding of the innovation system and the learning processes involved in a very specific cultural field: the production of early music.

Design/methodology/approach

A single case study of the generic value chain in the music production industry describes and analyses the process and the actors involved in editing a new early music collection resulting from the collaboration between a record company and a public research organization.

Findings

There is a need for new knowledge in the various stages of performance and publication of a new recording. The early music sector is a knowledge‐intensive, science‐driven sector that can be characterized as a system because the interactions among actors substantially influence final products.

Research limitations/implications

The single case study represents a specific sector within the music industry. However, its conclusions can be applied to other fields in the cultural heritage sector.

Originality/value

The literature on innovation in the cultural field primarily focuses on the relationship between art and information and communication technology (ICT). This paper is novel in analysing a case where scientific knowledge is key to new product development, and suggesting that we need to take account of the interactions among cultural heritage entities, universities and other knowledge production organizations. It concludes that these organizations should be involved institutionally in other aspects of the innovation process.
Quality expectations in Thai higher education institutions: multiple stakeholder perspectives
Authors: Sandmaung Mukdashine , Ba Khang Do
Published: July 2013

Purpose

The purpose of this paper is to determine the quality indicators that are suitable for assuring quality in higher education institutions (HEIs) in Thailand, from the perspectives of students, teaching staff, managerial staff and employers. These quality expectations are then to be compared with the quality indicators of the Office of Higher Education Commission (OHEC) in Thailand.

Design/methodology/approach

A list of quality expectations was first established based on a review of the literature and the OHEC quality indicators, then finalized with input from interviews with experts and a pilot survey. Over 2,000 questionnaires were then delivered to stakeholders. Statistical analyses of differences and factors were conducted and compared with OHEC's official list of quality indicators.

Findings

In terms of relative perceived importance, while the rank correlations among quality expectations of different stakeholder groups were generally high, a correlation between students and teaching/managerial staff was the lowest. The most important expectations of stakeholders are found missing from the OHEC list, and several indicators used by OHEC were among the lowest ranked items. Factor analysis also suggested a different structure for categorizing the quality indicators compared to what is being mandated by OHEC.

Practical implications

The stakeholders' perspectives and their priorities observed in this study could serve as initial empirical inputs for OHEC and HEIs in developing or reviewing quality assurance systems.

Originality/value

The paper shows that assessing and comparing similarities and differences among multiple stakeholder perspectives can present a more comprehensive and accurate understanding for the development of quality assurance indicators suitable for Thai HEIs.
Immunogenicity and safety of H1N1 influenza hemagglutinin protein expressed in a baculovirus system
Authors: Na Ha‐Na , Kim Kyung Hyun , Song Man Ki , Park Hye‐lim , Lee Eun‐young , Shim Seung‐Hyun , Park Sooho , Nam Jae‐Hwan
Published: July 2013

Although most influenza vaccines are produced in eggs, new types of vaccines must be developed. In this study, the immunogenicity and safety of a baculovirus‐expressed hemagglutinin (HA) of H1N1 influenza virus (Korea/01/2009; designated “HA‐Bac‐K”) was compared with those of a commercially available baculovirus‐expressed HA (designated “HA‐Bac‐C”) and an Escherichia coli‐expressed HA (designated “HA‐E. Coli‐K”). HA‐Bac‐K succeeded in inducing hemagglutination inhibition and neutralization antibodies in mouse and ferret models. The different immunogenicities observed may be attributable to the different expression systems and purification protocols used. Our work suggests that HA expressed in a baculovirus system is an effective and safe candidate influenza vaccine.
Speech and language therapy students: how do those with ‘non traditional’ university entry qualifications perform?
Authors: Smith Christina H. , Mahon Matt , Newton Caroline
Published: July 2013

Background

Entry to speech and language therapy (SLT) undergraduate pre‐registration programmes in the UK is usually achieved through qualifications attained at school (e.g. A‐levels). A smaller number of people who did not succeed academically at school enter through qualifications achieved post‐schooling, e.g. Access to Higher Education courses. This second group of students are attractive recruits to SLT training programmes as they bring different experiences and backgrounds to the programme and to the SLT profession. However, there are no published studies that explore the academic performance or degree and employment outcomes of students who enter university through this route.

Aims

To investigate the success of non‐traditional entry students, specifically those with Access qualifications, on one pre‐registration SLT undergraduate degree at a university in the UK. Success is measured in terms of module results, obtaining a degree, the ability to register as an SLT and first employment destination post‐graduation.

Methods & Procedures

University applications were reviewed and students who entered via an Access course were identified for a ten‐year period. Results for modules, individual assessments and final degree classification were obtained from their files and were compared against the mean score of traditional entry students on the years of the course covered by this study using a series of one sample t‐tests.

Outcomes & Results

The students who entered through the non‐traditional academic route are generally successful in completing their degree and in registering to work as an SLT. However, as a group they perform less well on the degree overall, on individual modules and on written examinations and they do not close this performance gap over the four years of the degree. In contrast, however, they perform on a par with their peers on other types of assessment (e.g. clinical placement, case study, data exercise). The Access students were more likely than traditional entry students to be working as an SLT six‐months post‐qualification.

Conclusions & Implications

These results suggest that some types of assessment may be more effective at tapping the skills of students from non‐traditional academic backgrounds, and that more tailored support could be provided for this group of students in tackling university written examinations throughout all years of the programme. Despite limited preparation for university‐level study and mixed performance on assessment, students from non‐traditional academic backgrounds can do well at university and successfully gain employment as SLTs.