bluuebunny/crossref_metadata_2025 huggingface dataset:
Interactive Effects of Light Quality during Day Extension and Temperature on Bud Set, Bud Burst and PaFTL2, PaCOL1-2 and PaSOC1 Expression in Norway Spruce (Picea abies (L.) Karst.)
Authors: Chiang Camilo , Viejo Marcos , Aas Oda Toresdatter , Hobrak Katharina T. , Strømme Christian Bianchi , Fløistad Inger Sundheim , Olsen Jorunn E.
Published: March 2021

Light and temperature are crucial factors for the annual growth rhythm of tree seedlings of the boreal and temperate zone. Dormant, vegetative winter buds are formed under short days (SD) and altered light quality. In the conifer Norway spruce, expression of FTL2 increases and PaCOL1-2 and PaSOC1 decrease under light regimes, inducing bud set. Although temperature is known to modulate the timing of bud set, information about combined effects of light climate and temperature on bud phenology and gene expression is limited. We studied the interactive effects of temperature (18, 22/24 °C) and day extension with blue (B), red (R) or far-red (FR) light or different R:FR ratios compared to SD on growth–dormancy cycling and expression of FTL2, PaCOL1-2 and PaSOC1 in Norway spruce seedlings. Day-extension with B light and all treatments involving FR light sustained shoot elongation, with increased growth at higher temperature. The R light treatment resulted in delayed/prevented bud set compared to SD, with more delay/prevented bud set at 24 °C than 18 °C. This was associated with lower PaFTL2-transcript levels at 24 °C and more rapid subsequent bud burst. For the growth-sustaining treatments (long days, FR and B light), the PaFTL2-transcript levels were generally lower and those of PaCO1-2 and PaSOC1 higher compared with SD and R light. In conclusion, our results demonstrate more reduced/prevented bud set and faster bud burst with increased temperature under day extension with R light, indicating less deep dormancy than at lower temperature. Also, sustained shoot elongation under the B light treatment (27 µmol m−2 s−1) in contrast to the lower B light-irradiances tested previously (≤13 µmol m−2 s−1), demonstrates an irradiance-dependent effect of day extension with B light.
Very High Gravity Bioethanol Revisited: Main Challenges and Advances
Authors: Gomes Daniel , Cruz Mariana , de Resende Miriam , Ribeiro Eloízio , Teixeira José , Domingues Lucília
Published: March 2021

Over the last decades, the constant growth of the world-wide industry has been leading to more and more concerns with its direct impact on greenhouse gas (GHG) emissions. Resulting from that, rising efforts have been dedicated to a global transition from an oil-based industry to cleaner biotechnological processes. A specific example refers to the production of bioethanol to substitute the traditional transportation fuels. Bioethanol has been produced for decades now, mainly from energy crops, but more recently, also from lignocellulosic materials. Aiming to improve process economics, the fermentation of very high gravity (VHG) mediums has for long received considerable attention. Nowadays, with the growth of multi-waste valorization frameworks, VHG fermentation could be crucial for bioeconomy development. However, numerous obstacles remain. This work initially presents the main aspects of a VHG process, giving then special emphasis to some of the most important factors that traditionally affect the fermentation organism, such as nutrients depletion, osmotic stress, and ethanol toxicity. Afterwards, some factors that could possibly enable critical improvements in the future on VHG technologies are discussed. Special attention was given to the potential of the development of new fermentation organisms, nutritionally complete culture media, but also on alternative process conditions and configurations.
Cordyceps cicadae NTTU 868 Mycelium with The Addition of Bioavailable Forms of Magnesium from Deep Ocean Water Prevents the Aβ40 and Streptozotocin-Induced Memory Deficit via Suppressing Alzheimer’s Disease Risk Factors and Increasing Magnesium Uptake of Brain
Authors: Wu Yan-Zhong , Lee Chun-Lin
Published: March 2021

Alzheimer’s disease (AD) is a common neurodegenerative disease characterized by continuous accumulation of β-amyloid (Aβ) in the brain. Deep ocean water (DOW) with rich inorganic salts and minerals was proven to promote fungi growth and metabolism. Cordyceps cicada, a functional food fungus, can produce higher anti-oxidant and anti-inflammatory compounds including adenosine, polysaccharide, and N(6)-(2-Hydroxyethyl) adenosine (HEA). This study used DOW as the culture water of C. cicadae NTTU 868 for producing DOW-cultured C. cicadae (DCC), and further investigated the effects and mechanisms on improving the memory deficit and repressing risk factors expressions in Aβ40 and streptozotocin (STZ)-induced Alzheimer’s disease rats model. In the results, DCC including mycelium and filtrate had adenosine, HEA, polysaccharide, and intracellular Mg2+ after fermentation with DOW. DCC had more effect on the improvement of memory deficit because it suppressed Aβ40 and streptozotocin (STZ) infusion caused BACE, pro-inflammatory factors expressions, and Aβ40 accumulation by increasing sRAGE expression in the brain. Furthermore, DCC enhanced the MAGT1 expression due to high organic magnesium, which can reverse Aβ40-induced cortex magnesium deficiency and further repress Aβ40 accumulation.
Deep Model Poisoning Attack on Federated Learning
Authors: Zhou Xingchen , Xu Ming , Wu Yiming , Zheng Ning
Published: March 2021

Federated learning is a novel distributed learning framework, which enables thousands of participants to collaboratively construct a deep learning model. In order to protect confidentiality of the training data, the shared information between server and participants are only limited to model parameters. However, this setting is vulnerable to model poisoning attack, since the participants have permission to modify the model parameters. In this paper, we perform systematic investigation for such threats in federated learning and propose a novel optimization-based model poisoning attack. Different from existing methods, we primarily focus on the effectiveness, persistence and stealth of attacks. Numerical experiments demonstrate that the proposed method can not only achieve high attack success rate, but it is also stealthy enough to bypass two existing defense methods.
Fire from the Sky in the Anthropocene
Authors: Fernandes Paulo M. , Santos João A. , Castedo-Dorado Fernando , Almeida Rui
Published: March 2021

Lightning-caused fires (LCFs) and fire environments influenced by thunderstorms are increasingly implicated in extreme wildfire events around the world, with devastating consequences to society and the environment. However, the disaster potential inherent to LCFs is often neglected, especially where the fire regime is determined mostly by anthropogenic ignitions. Such disconnect between perceived risk and actual risk is illustrated with the Iberian Peninsula, where thunderstorm-driven wildfires are comparatively rare but have resulted in large-scale burning and considerable loss of human life. Even low LCF regions should embrace fire management strategies able to cope with LCFs.
Heat Transfer Study of the Ferrofluid Flow in a Vertical Annular Cylindrical Duct under the Influence of a Transverse Magnetic Field
Authors: Bakalis Panteleimon A. , Papadopoulos Polycarpos K. , Vafeas Panayiotis
Published: March 2021

We studied the laminar fully developed ferrofluid flow and heat transfer phenomena of an otherwise magnetic fluid into a vertical annular duct of circular cross-section and uniform temperatures on walls which were subjected to a transverse external magnetic field. A computational algorithm was used, which coupled the continuity, momentum, energy, magnetization and Maxwell’s equations, accompanied by the appropriate conditions, using the continuity–vorticity–pressure (C.V.P.) method and a non-uniform grid. The results were obtained for different values of field strength and particles’ volumetric concentration, wherein the effects of the magnetic field on the ferrofluid flow and the temperature are revealed. It is shown that the axial velocity distribution is highly affected by the field strength and the volumetric concentration, the axial pressure gradient depends almost linearly on the field strength, while the heat transfer significantly increases due to the generated secondary flow.
Biofilm-Forming Ability of Microbacterium lacticum and Staphylococcus capitis Considering Physicochemical and Topographical Surface Properties
Authors: Zand Elena , Pfanner Hedwig , Domig Konrad J. , Sinn Gerhard , Zunabovic-Pichler Marija , Jaeger Henry
Published: March 2021

Biofilm characteristics of Microbacterium lacticum D84 (M. lacticum) and Staphylococcus capitis subsp. capitis (S. capitis) on polytetrafluoroethylene and AISI-304 stainless steel at early- (24, 48 h) and late-stage (144, 192 h) biofilm formation were investigated. M. lacticum biofilm structure was more developed compared to S. capitis, representing vastly mature biofilms with a strongly developed amorphous matrix, possibly extracellular polymeric substances (EPSs), at late-stage biofilm formation. S. capitis showed faster growth behavior but still resulted in a relatively flat biofilm structure. Strong correlations were found between several roughness parameters and S. capitis surface coverage (r ≥ 0.98), and between total surface free energy (γs) and S. capitis surface coverage (r = 0.89), while M. lacticum remained mostly unaffected. The pronounced ubiquitous biofilm characteristics make M. lacticum D84 a suitable model for biofilm research. Studying biofilm formation of these bacteria may help one understand bacterial adhesion on interfaces and hence reduce biofilm formation in the food industry.
Innovative Fermented Beverages Made with Red Rice, Barley, and Buckwheat
Authors: Cardinali Federica , Osimani Andrea , Milanović Vesna , Garofalo Cristiana , Aquilanti Lucia
Published: March 2021

The increase in food intolerances, allergies, and food-based lifestyle choices has dramatically increased the consumer demand for healthy foods characterized by pleasant sensory traits. In such a context, innovative cereal-based beverages are characterized by high nutritional value, pleasant palatability, and potential healthy properties. In the present study, a pool of 23 lactic acid bacteria strains was preliminary assayed as monocultures for the fermentation of three ad hoc formulated cereal- (red rice and barley) and pseudocereal (buckwheat) -based substrates. Eight strains with the best performance in terms of acidification rate were selected for the formulation of three multiple strain cultures to be further exploited for the manufacture of laboratory-scale prototypes of fermented beverages. The compositional and microbiological features of the three experimental beverages highlighted their high biological value for further exploitation.
Unripe Papaya By-Product: From Food Wastes to Functional Ingredients in Pancakes
Authors: Joymak Waralee , Ngamukote Sathaporn , Chantarasinlapin Praew , Adisakwattana Sirichai
Published: March 2021

Papaya is one of the most economic and valuable fruits in tropical countries. However, the fruit processing industries generate a high volume of unripe papaya waste and by-products. To reduce this waste, unripe papaya powder (UPP) was manufactured and incorporated into pancake formulation. The results showed that a particle size of UPP was 140.8 ± 2.1 µm, which contained polyphenolic compounds, dietary fiber and demonstrated ferric reducing antioxidant power (FRAP). Compared with wheat flour, UPP had higher values of water absorption index, water solubility index and swelling index and lower level of amylose. In the cholesterol-reducing effect, UPP decreased the formation of cholesterol micellization and bound bile acids. Interestingly, incorporation of 5–20% UPP into pancakes could decrease the glucose release with a concomitant increase in the percentage of undigestible starch. The hardness and chewiness of pancake was increased with a higher amount of UPP (10–20%). The results suggest that UPP from fruit processing waste can be regarded as a promising functional ingredient to incorporate with pancakes.
Impact of Rapeseed Press Cake on the Rheological Properties and Expansion Dynamics of Extruded Maize Starch
Authors: Martin Anna , Osen Raffael , Karbstein Heike Petra , Emin M. Azad
Published: March 2021

Rapeseed press cake (RPC), an oil pressing side product rich in protein and fiber, can be combined with starch and valorized into directly expanded products using extrusion technology. The mechanism of starch expansion has been studied in detail, but the impact of RPC on expansion behavior is poorly understood. However, it can be linked to rheological and physicochemical properties and is a key product quality parameter. Blends with different amounts of RPC (0, 10, 40 g/100 g) were extruded at different barrel temperatures (100, 120, 140 °C) and moisture contents (24 or 29 g/100 g). The initial, intermediate and final sectional, longitudinal and volumetric expansion indices (SEI, LEI, VEI) were monitored directly, 10 s and 24 h after die exit to measure extrudate growth and shrinkage. The viscous and elastic properties of the extruded blends were investigated in a closed cavity rheometer. Starch and blends with 10 g/100 g RPC achieved a high initial SEI followed by significant short-term shrinkage. Blends containing 40 g/100 g RPC did not show any initial expansion. With increasing RPC content, the intermediate SEI decreased, but all samples reached a similar final SEI due to time-dependent swelling of the RPC blends. With increasing RPC content, the elasticity of the starch-based extruded samples significantly increased. Our study shows that comprehensive control and understanding of expansion mechanisms can be achieved only by investigating all stages of extrudate growth and shrinkage. We also found that the closed cavity rheometer is a powerful tool to correlate the rheological properties and expansion mechanisms of biopolymers.
Impact of the Resistance Responses to Stress Conditions Encountered in Food and Food Processing Environments on the Virulence and Growth Fitness of Non-Typhoidal Salmonellae
Authors: Guillén Silvia , Nadal Laura , Álvarez Ignacio , Mañas Pilar , Cebrián Guillermo
Published: March 2021

The success of Salmonella as a foodborne pathogen can probably be attributed to two major features: its remarkable genetic diversity and its extraordinary ability to adapt. Salmonella cells can survive in harsh environments, successfully compete for nutrients, and cause disease once inside the host. Furthermore, they are capable of rapidly reprogramming their metabolism, evolving in a short time from a stress-resistance mode to a growth or virulent mode, or even to express stress resistance and virulence factors at the same time if needed, thanks to a complex and fine-tuned regulatory network. It is nevertheless generally acknowledged that the development of stress resistance usually has a fitness cost for bacterial cells and that induction of stress resistance responses to certain agents can trigger changes in Salmonella virulence. In this review, we summarize and discuss current knowledge concerning the effects that the development of resistance responses to stress conditions encountered in food and food processing environments (including acid, osmotic and oxidative stress, starvation, modified atmospheres, detergents and disinfectants, chilling, heat, and non-thermal technologies) exerts on different aspects of the physiology of non-typhoidal Salmonellae, with special emphasis on virulence and growth fitness.
Enrichment of Broiler Chickens’ Meat with Dietary Linseed Oil and Lysine Mixtures: Influence on Nutritional Value, Carcass Characteristics and Oxidative Stress Biomarkers
Authors: El-Bahr Sabry M. , Shousha Saad , Alfattah Mohamed A. , Al-Sultan Saad , Khattab Wasseem , Sabeq Islam I. , Ahmed-Farid Omar , El-Garhy Osama , Albusadah Khalid A. , Alhojaily Sameer , Shehab Ahmed
Published: March 2021

This study aimed to evaluate the effect of four combinations of dietary linseed oil and lysine mixtures on performance, fatty and amino acid profiles, oxidative stress biomarkers, cell energy and meat quality parameters of broiler chickens. One hundred and sixty broiler chicks were allocated into four groups. Birds of groups 1–4 were fed diets containing optimum lysine and 2% of linseed oil, optimum lysine and 4% of linseed oil, high lysine and 2% of linseed oil, and high lysine and 4% of linseed oil, respectively, for a period of 35 days. High linseed oil or lysine levels did not affect the performance of the tested birds, but the high level of dietary linseed oil decreased the concentrations of muscles’ saturated fatty acids (SFA). The highest values of ω-3 polyunsaturated fatty (ω-3 PUFA) and arachidonic acids with lowest levels of monounsaturated fatty (MUFA) were detected in the muscles of birds fed diets containing high linseed oils and/or lysine levels. High linseed oil or lysine levels provided the best essential amino acid profile and improved antioxidant components as well as cell energy, and tenderness and redness of the meat. Conclusively, high dietary lysine and linseed oil combinations improved the nutritional value, antioxidant status and cell energy of broiler chickens’ meat.
Use of Chia by-Products Obtained from the Extraction of Seeds Oil for the Development of New Biodegradable Films for the Agri-Food Industry
Authors: Muñoz-Tebar Nuria , Molina Ana , Carmona Manuel , Berruga María Isabel
Published: March 2021

Chia oil production and consumption have increased in recent years, producing a large number of by-products that had low utility or economic value for the industry. In this study, a biodegradable film was successfully prepared from mucilage extracted from defatted chia flour. The physical-chemical, optical, water vapor permeability (WVP), and mechanical properties of films made with two different types of chia matrixes (defatted flour and whole seeds) were determined. In general, defatted chia flour films exhibited a slightly reddish and yellowish color but still transparent in appearance, were good visible light barriers, and had better mechanical properties than films made with whole seeds. They also have greater WVP values than synthetic films such as low-density polyethylene. The results of the present study demonstrated that defatted chia flour can be used in producing edible films with improved quality characteristics.
The Creaming of Short Doughs and Its Impact on the Quality Attributes of Rotary-Molded Biscuits
Authors: Molina M. , Vaz Sandra , Bouchon Pedro
Published: March 2021

Scant attention has been given to understanding the impact of creaming stability on the final structure of semi-sweet biscuits, an aspect that has traditionally concerned the biscuit industry. Accordingly, the aim of this study was to analyze the influence of the creaming phase stability on the quality attributes of rotary-molded biscuits. Doughs were formulated with 10.2% of fat (wet basis) and 16.3% of sucrose (w.b.), using two sucrose particle sizes, which were either added directly or after dilution in water at different concentrations. Additionally, the creaming phase was prepared using either a low-shear or a high-shear mixer. The results show that an aqueous-phase migration occurred when the creaming was blended in a low-shear mixer, when using either powdered sucrose or granular sucrose diluted in water at a high concentration. The phase separation was inhibited with the high-shear mixer, which provided a stable creaming. Notwithstanding the variation in creaming stability, no differences were observed in hardness, aeration, sweetness, color and noise intensity. Additionally, the micro-CT analysis revealed that biscuits had a similar microstructure (air porosity and thickness of biscuit walls) when they were prepared with either an unstable or a stable creaming phase. Consequently, creaming stability does not seem to affect the structure and the most relevant sensory attributes of rotary-molded biscuits under this set of experimental conditions, which are representative of those used by the industry for this product category.
An Insight by Molecular Sensory Science Approaches to Contributions and Variations of the Key Odorants in Shiitake Mushrooms
Authors: Wang Si-Lu , Lin Song-Yi , Du Han-Ting , Qin Lei , Lei Li-Ming , Chen Dong
Published: March 2021

An insight using molecular sensory science approaches to the contributions and variations of the key odorants in shiitake mushrooms is revealed in this study. Odorants were extracted by headspace solid phase microextraction (HS-SPME) and direct solvent extraction combined with solvent-assisted flavor evaporation (DSE-SAFE) in fresh and hot-air-dried shiitake mushrooms. Among them, 18 and 22 predominant odorants were determined by detection frequency analysis (DFA) and aroma extract dilution analysis (AEDA) combined with gas chromatography-olfactometry (GC-O) in the fresh and dried samples, respectively. The contributions of these predominant odorants in the food matrix were determined by quantification and odor activity values (OAVs) with aroma recombination verification. There were 13 and 14 odorants identified as key contributing odorants to overall aroma, respectively. 1-Octen-3-ol and 1-octen-3-one were the most key contributing odorants in the fresh samples in contributing mushroom-like odor. After hot-air-drying, the OAV and concentrations on dry basis of the key contributing odorants changed, due to oxidation, degradation, caramelization and Maillard reactions of fatty acids, polysaccharides and amino acids. 1-Octen-3-ol was reduced most significantly and degraded to 1-hydroxy-3-octanone, while phenylethyl alcohol increased the most and was formed by phenylalanine. In hot-air-dried samples, lenthionine became the most important contributor and samples were characterized by a sulfury odor. Overall contributions and variations of odorants to the aroma of shiitake mushrooms were revealed at the molecular level.
Structural Violence of Schooling: A Genealogy of a Critical Family History of Three Generations of African American Women in a Rural Community in Florida
Authors: Phillips Evelyn Newman , Gichiru Wangari
Published: March 2021

Through the lens of structural violence, Black feminism and critical family history, this paper explores how societal structures informed by white supremacy shaped the lives of three generations of rural African American women in a family in Florida during the middle to the late twentieth century. Specifically, this study investigates how disparate funding, segregation, desegregation, poverty and post-desegregation policies shaped and limited the achievement trajectories among these women. Further, an oral historical examination of their lives reveals the strategies they employed despite their under-resourced and sometimes alienating schooling. The paper highlights the experiences of the Newman family, descendants of captive Africans in the United States that produced three college-educated daughters and a granddaughter despite structural barriers that threatened their progress. Using oral history interviews, archival resources and first-person accounts, this family’s story reveals a genealogy of educational achievement, barriers and agency despite racial and gendered limitations in a Southern town. The findings imply that their schooling mirrors many of the barriers that other Blacks face. However, this study shows that community investment in African American children, plus teachers that affirm students, and programs such as Upward Bound, help to advance Black students in marginalized communities. Further, these women’s lives suggest that school curriculums need to be anti-racist and public policies that affirm each person regardless of the color of their skin. A simple solution that requires the structural violence of whiteness be eliminated from the schooling spheres.
Set-Based Rare Variant Expression Quantitative Trait Loci in Blood and Brain from Alzheimer Disease Study Participants
Authors: Patel Devanshi , Zhang Xiaoling , Farrell John J. , Lunetta Kathryn L. , Farrer Lindsay A.
Published: March 2021

Because studies of rare variant effects on gene expression have limited power, we investigated set-based methods to identify rare expression quantitative trait loci (eQTL) related to Alzheimer disease (AD). Gene-level and pathway-level cis rare-eQTL mapping was performed genome-wide using gene expression data derived from blood donated by 713 Alzheimer’s Disease Neuroimaging Initiative participants and from brain tissues donated by 475 Religious Orders Study/Memory and Aging Project participants. The association of gene or pathway expression with a set of all cis potentially regulatory low-frequency and rare variants within 1 Mb of genes was evaluated using SKAT-O. A total of 65 genes expressed in the brain were significant targets for rare expression single nucleotide polymorphisms (eSNPs) among which 17% (11/65) included established AD genes HLA-DRB1 and HLA-DRB5. In the blood, 307 genes were significant targets for rare eSNPs. In the blood and the brain, GNMT, LDHC, RBPMS2, DUS2, and HP were targets for significant eSNPs. Pathway enrichment analysis revealed significant pathways in the brain (n = 9) and blood (n = 16). Pathways for apoptosis signaling, cholecystokinin receptor (CCKR) signaling, and inflammation mediated by chemokine and cytokine signaling were common to both tissues. Significant rare eQTLs in inflammation pathways included five genes in the blood (ALOX5AP, CXCR2, FPR2, GRB2, IFNAR1) that were previously linked to AD. This study identified several significant gene- and pathway-level rare eQTLs, which further confirmed the importance of the immune system and inflammation in AD and highlighted the advantages of using a set-based eQTL approach for evaluating the effect of low-frequency and rare variants on gene expression.
Nisyros Volcanic Island: A Geosite through a Tailored GIS Story
Authors: Antoniou Varvara , Nomikou Paraskevi , Panousis Dimitrios , Zafeirakopoulou Effrosyni
Published: March 2021

The volcanic island of Nisyros (Greece) is here presented as it presents unique characteristics being a Quaternary volcano in the eastern Aegean Sea, composed of volcanic rocks, and featuring a central caldera that is surrounded by volcanic domes and thick lavas. Its history is strongly connected to the volcano impressing the visitors with steaming hydrothermal craters, intensive smell of sulfur and fumarolic gases, and hot springs. Due to its morphology and geographical position, its cultural and historical heritage has been unchanged in time, bequeath to the island plenty of churches, monasteries with hagiographic frescoes, castles, caves, and spas. To present the geodiversity, the cultural environment, and the biodiversity of this geosite, a relatively new geographic approach was used, ESRI Story Maps. Being web-based applications, they are widespread as an interactive responsive tool used for spatial data communication and dissemination, by combining thematic 2D and 3D webmaps, narrative text, and multimedia content. Such applications can be an ideal way for presenting the available information of places characterized as geosites or protected areas worldwide, providing quick access to the available information to a broader, non-technical audience, developing the interest, and possibly motivating the public to learn more or visit them.
On the Network Transmission Mechanisms of Disease-Specific Healthcare Expenditure Spillovers: Evidence from the Connectedness Network Analyses
Authors: Chen Wen-Yi
Published: March 2021

Previous studies investigating factors influencing healthcare expenditure growth ignored the network transmission mechanisms of disease-specific healthcare expenditure spillovers and regarded the processes culminating in healthcare expenditure growth as a black box. In this study, we investigated factors influencing the network transmission mechanisms underlying the determinants of healthcare expenditure growth through the dynamic connectedness network and the robust least square regression analyses. Our results indicate that demographic transition and business cycles are key factors increasing interconnectedness of different disease-specific healthcare expenditures, and that promotion of primary care utilization would reduce total healthcare expenditure spillovers. In order to reduce diffusion of disease-specific healthcare expenditures, health promotion activities should focus on those clinical diagnosis-related groups of diseases classified as pure net transmitters of spillover, and preventive interventions targeting different diseases should be activated in different phrases of the business cycle.
Characteristics of Osteoporosis & Osteoporotic Fractures in Korea Based on Health Insurance Review and Assessment (HIRA) Database: 2009–2017
Authors: Oh Ho-Seok , Kim Sung-Kyu , Seo Hyoung-Yeon
Published: March 2021

To investigate the incidence and characteristics of osteoporosis and osteoporotic fractures in Korea, we used the Health Insurance Review and Assessment Service (HIRA) database. Patients over 50 years old, who were diagnosed or treated for osteoporosis and osteoporotic fractures in all hospitals and clinics, were analyzed between 1 January 2009 and 31 December 2017 by using the HIRA database that contains prescription data and diagnostic codes. These data were retrospectively analyzed by decade and age-specific and gender-specific incidents in each year. We also evaluated other characteristics of patients including medication state of osteoporosis, primary used medical institution, regional-specific incidence of osteoporosis, and incidence of site-specific osteoporotic fractures. The number of osteoporosis patients over 50 years old, as diagnosed by a doctor, steadily increased from 2009 to 2017. The number of osteoporosis patients was notably greatest in the 60′s and 70′s age groups in every study period. Patients undergoing treatment for osteoporosis increased significantly (96%) from 2009 to 2017. Among the patients diagnosed with osteoporosis, the proportion who experienced osteoporotic fracture increased gradually (60%) from 2009 to 2017. The number of patients with osteoporotic fractures of the spine and hip was highest in the 70 to 90 age range, and the number of patients with osteoporotic fractures in the upper and lower extremities was highest in the 50 to 70 age range. Understanding the trends of osteoporosis in Korea will contribute to manage the increased number of patients with osteoporosis and osteoporotic fractures.
Developing Bilingualism in Nursing Students: Learning Foreign Languages beyond the Nursing Curriculum
Authors: Dos Santos Luis M.
Published: March 2021

Nursing curriculum usually focuses on vocational development to train students to become nursing professionals after graduation. However, due to the packed major schedule and curriculum, many students are not required to take additional foreign language courses for their associate degree. Based on the lens of social cognitive career theory, the researcher sought to understand the motivations and reasons behind the learning behaviours. One research question was guided in this study, which was, what are the motivations and reasons for taking foreign language courses beyond their (i.e., nursing students) major curriculum and coursework plan? A qualitative research method was employed to collect interview data from 60 nursing students. The finding of this study indicated that the interest in career development and personal consideration were two of the most important factors for foreign language learning for these groups of nursing students. The results of this study provided recommendations for college leaders, government agencies, and policymakers to reform and polish foreign language courses and offer directions to contemporary students of the nursing curriculum. Students may also be benefitted as the study outlined the motivations and reasons for foreign language learning. Therefore, all parties may take this study as a blueprint to exercise their future developments.
Human Bone Marrow Mesenchymal Stromal Cell-Derived CXCL12, IL-6 and GDF-15 and Their Capacity to Support IgG-Secreting Cells in Culture Are Divergently Affected by Doxorubicin
Authors: Lasaviciute Gintare , Höbinger Anna , Ujvari Dorina , Salamon Daniel , Yusuf Aisha , Sundin Mikael , Sverremark-Ekström Eva , Chikhi Rayan , Nilsson Anna , Saghafian-Hedengren Shanie
Published: March 2021

Various subsets of bone marrow mesenchymal stromal cells (BM MSCs), including fibroblasts, endothelial, fat and reticular cells, are implicated in the regulation of the hematopoietic microenvironment and the survival of long-lived antibody-secreting cells (ASCs). Nowadays it is widely acknowledged that vaccine-induced protective antibody levels are diminished in adults and children that are treated for hematological cancers. A reason behind this could be damage to the BM MSC niche leading to a diminished pool of ASCs. To this end, we asked whether cell cytotoxic treatment alters the capacity of human BM MSCs to support the survival of ASCs. To investigate how chemotherapy affects soluble factors related to the ASC niche, we profiled a large number of cytokines and chemokines from in vitro-expanded MSCs from healthy donors or children who were undergoing therapy for acute lymphoblastic leukemia (ALL), following exposure to a widely used anthracycline called doxorubicin (Doxo). In addition, we asked if the observed changes in the measured soluble factors after Doxo exposure impacted the ability of the BM niche to support humoral immunity by co-culturing Doxo-exposed BM MSCs with in vitro-differentiated ASCs from healthy blood donors, and selective neutralization of cytokines. Our in vitro results imply that Doxo-induced alterations in BM MSC-derived interleukin 6 (IL-6), CXCL12 and growth and differentiation factor 15 (GDF-15) are not sufficient to disintegrate the support of IgG-producing ASCs by the BM MSC niche, and that serological memory loss may arise during later stages of ALL therapy.
Response Mechanism of Plants to Drought Stress
Authors: Yang Xinyi , Lu Meiqi , Wang Yufei , Wang Yiran , Liu Zhijie , Chen Su
Published: March 2021

With the global climate anomalies and the destruction of ecological balance, the water shortage has become a serious ecological problem facing all mankind, and drought has become a key factor restricting the development of agricultural production. Therefore, it is essential to study the drought tolerance of crops. Based on previous studies, we reviewed the effects of drought stress on plant morphology and physiology, including the changes of external morphology and internal structure of root, stem, and leaf, the effects of drought stress on osmotic regulation substances, drought-induced proteins, and active oxygen metabolism of plants. In this paper, the main drought stress signals and signal transduction pathways in plants are described, and the functional genes and regulatory genes related to drought stress are listed, respectively. We summarize the above aspects to provide valuable background knowledge and theoretical basis for future agriculture, forestry breeding, and cultivation.
Effects of River Discharge and Sediment Load on Sediment Plume Behaviors in a Coastal Region: The Yukon River, Alaska and the Bering Sea
Authors: Chikita Kazuhisa A. , Wada Tomoyuki , Kudo Isao , Saitoh Sei-Ichi , Toratani Mitsuhiro
Published: March 2021

In the Bering Sea around and off the Yukon River delta, surface sediment plumes are markedly formed by glacier-melt and rainfall sediment runoffs of the Yukon River, Alaska, in June– September. The discharge and sediment load time series of the Yukon River were obtained at the lowest gauging station of US Geological Survey in June 2006–September 2010. Meanwhile, by coastal observations on boat, it was found out that the river plume plunges at a boundary between turbid plume water and clean marine water at the Yukon River sediment load of more than ca. 2500 kg/s. Grain size analysis with changing salinity (‰) for the river sediment indicated that the suspended sediment becomes coarse at 2 to 5‰ by flocculation. Hence, the plume’s plunging probably occurred by the flocculation of the Yukon suspended sediment in the brackish zone upstream of the plunging boundary, where the differential settling from the flocculation is considered to have induced the turbid water intrusion into the bottom layer.
Insight into Kytococcus schroeteri Infection Management: A Case Report and Review
Authors: Bagelman Shelly , Zvigule-Neidere Gunda
Published: March 2021

Background: Kytococcus schroeteri is a member of normal skin microflora, which can cause lethal infections in immunosuppressed hosts. In this review we attempted to draw patterns of its pathogenicity, which seem to vary regarding host immune status and the presence of implantable devices. Evidence suggests this pathogen houses many resistance-forming proteins, which serve to exacerbate the challenge in curing it. Available information on K. schroeteri antibacterial susceptibility is scarce. In this situation, a novel, genome-based antibiotic resistance analysis model, previously suggested by Su et al., could aid clinicians dealing with unknown infections. In this study we merged data from observed antibiotic resistance patterns with resistance data demonstrated by DNA sequences. Methods: We reviewed all available articles and reports on K. schroeteri, from peer-reviewed online databases (ClinicalKey, PMC, Scopus and WebOfScience). Information on patients was then subdivided into patient profiles and tabulated independently. We later performed K. schroeteri genome sequence analysis for resistance proteins to understand the trends K. schroeteri exhibits. Results: K. schroeteri is resistant to beta-lactams, macrolides and clindamycin. It is susceptible to aminoglycosides, tetracyclines and rifampicin. We combined data from the literature review and sequence analysis and found evidence for the existence of PBP, PBP-2A and efflux pumps as likely determinants of K. schroeteri. Conclusions: Reviewing the data permits the speculation that baseline immune status plays a role in the outcome of a Kytococcal infection. Nonetheless, our case report demonstrates that the outcome of a lower baseline immunity could still be favorable, possibly using rifampicin in first-line treatment of infection caused by K. schroeteri.
Tracking the Deposition and Sources of Soil Carbon and Nitrogen in Highly Eroded Hilly-Gully Watershed in Northeastern China
Authors: Li Na , Zhang Yanqing , Sun Zhanxiang , Yang John , Liu Enke , Li Chunqian , Li Fengming
Published: March 2021

Understanding the deposition and tracking the source of soil organic carbon (C) and nitrogen (N) within agricultural watersheds are critical for assessing soil C and N budgets and developing watershed-specific best management practices. Few studies have been conducted and reported on highly eroded hilly-gully watersheds. In this field study, a constructed dam-controlled hilly-gully watershed in northeastern China was selected to identify the sources of soil C and N losses. Soils at various land uses and landscape positions, and sediments near the constructed dam, were collected and analyzed for selected physiochemical properties, total organic carbon (TOC), total nitrogen (TN), and stable isotopes (13C and 15N). Soil C and N loss and deposition in the watershed were assessed and the relative contributions of each source quantified by a stable isotope mixing model (SIAR). Results indicated that soil C loss was primarily from cropland, accounting for 58.75%, followed by gully (25.49%), forest (9.2%), and grassland (6.49%). Soil N loss was similar to soil C, with cropland contribution of 80.58%, gully of 10.30%, grassland of 7.54%, and forest of 1.59%. The C and N deposition gradually decreased along the direction of the runoff pathway near the constructed dam, and the deposited C and N from cropland and gullies showed an order: middle-dam > bottom-dam > upper-dam and upper-dam > bottom-dam > middle-dam, respectively. A high correlation between soil TOC or TN and the sediment properties suggested that the deposition conditions could be the major factors affecting the C and N pools in the sedimentary zones. This study would provide a scientific insight to develop effective management practices for soil erosion and nutrient loss control in highly eroded agriculture watersheds.
The Impact of Community Happenings in OpenStreetMap—Establishing a Framework for Online Community Member Activity Analyses
Authors: Schott Moritz , Grinberger Asher Yair , Lautenbach Sven , Zipf Alexander
Published: March 2021

The collaborative nature of activities in Web 2.0 projects leads to the formation of online communities. To reinforce this community, these projects often rely on happenings centred around data creation and curation activities. We suggest an integrated framework to directly assess online community member performance in a quantitative manner and applied it to the case study of OpenStreetMap. A set of mappers who participated in both field and remote mapping-related happenings was identified. To measure the effects of happenings, we computed attributes characterising the mappers’ contribution behaviour before and after the happenings and tested for significant impacts in relation to a control group. Results showed that newcomers to OpenStreetMap adopted a contribution behaviour similar to the contribution behaviour typical for the respective happening they attended: When contributing after the happening, newcomers who attended a remote mapping event tended to concentrate on creating new data with lower quality but high quantity in places foreign to their home region; newcomers who attended a field mapping event updated and enhanced existing local data with high accuracy. The behaviour of advanced mappers stayed largely unaffected by happenings. Unfortunately, our results did not reveal a positive effect on the community integration of newcomers through happenings.
The Role of TNF-α and Anti-TNF-α Agents during Preconception, Pregnancy, and Breastfeeding
Authors: Romanowska-Próchnicka Katarzyna , Felis-Giemza Anna , Olesińska Marzena , Wojdasiewicz Piotr , Paradowska-Gorycka Agnieszka , Szukiewicz Dariusz
Published: March 2021

Tumor necrosis factor-alpha (TNF-α) is a multifunctional Th1 cytokine and one of the most important inflammatory cytokines. In pregnancy, TNF-α influences hormone synthesis, placental architecture, and embryonic development. It was also shown that increased levels of TNF-α are associated with pregnancy loss and preeclampsia. Increased TNF-α levels in complicated pregnancy draw attention to trophoblast biology, especially migratory activity, syncytialisation, and endocrine function. Additionally, elevated TNF-α levels may affect the maternal-fetal relationship by altering the secretory profile of placental immunomodulatory factors, which in turn affects maternal immune cells. There is growing evidence that metabolic/pro-inflammatory cytokines can program early placental functions and growth in the first trimester of pregnancy. Furthermore, early pregnancy placenta has a direct impact on fetal development and maternal immune system diseases that release inflammatory (e.g., TNF-α) and immunomodulatory factors, such as chronic inflammatory rheumatic, gastroenterological, or dermatological diseases, and may result in an abnormal release of cytokines and chemokines in syncytiotrophoblasts. Pregnancy poses a challenge in the treatment of chronic disease in patients who plan to have children. The activity of the disease, the impact of pregnancy on the course of the disease, and the safety of pharmacotherapy, including anti-rheumatic agents, in pregnancy should be considered.
Probing the Structure and Function of the Cytosolic Domain of the Human Zinc Transporter ZnT8 with Nickel(II) Ions
Authors: Catapano Maria Carmen , Parsons Douglas S. , Kotuniak Radosław , Mladěnka Přemysl , Bal Wojciech , Maret Wolfgang
Published: March 2021

The human zinc transporter ZnT8 provides the granules of pancreatic β-cells with zinc (II) ions for assembly of insulin hexamers for storage. Until recently, the structure and function of human ZnTs have been modelled on the basis of the 3D structures of bacterial zinc exporters, which form homodimers with each monomer having six transmembrane α-helices harbouring the zinc transport site and a cytosolic domain with an α,β structure and additional zinc-binding sites. However, there are important differences in function as the bacterial proteins export an excess of zinc ions from the bacterial cytoplasm, whereas ZnT8 exports zinc ions into subcellular vesicles when there is no apparent excess of cytosolic zinc ions. Indeed, recent structural investigations of human ZnT8 show differences in metal binding in the cytosolic domain when compared to the bacterial proteins. Two common variants, one with tryptophan (W) and the other with arginine (R) at position 325, have generated considerable interest as the R-variant is associated with a higher risk of developing type 2 diabetes. Since the mutation is at the apex of the cytosolic domain facing towards the cytosol, it is not clear how it can affect zinc transport through the transmembrane domain. We expressed the cytosolic domain of both variants of human ZnT8 and have begun structural and functional studies. We found that (i) the metal binding of the human protein is different from that of the bacterial proteins, (ii) the human protein has a C-terminal extension with three cysteine residues that bind a zinc(II) ion, and (iii) there are small differences in stability between the two variants. In this investigation, we employed nickel(II) ions as a probe for the spectroscopically silent Zn(II) ions and utilised colorimetric and fluorimetric indicators for Ni(II) ions to investigate metal binding. We established Ni(II) coordination to the C-terminal cysteines and found differences in metal affinity and coordination in the two ZnT8 variants. These structural differences are thought to be critical for the functional differences regarding the diabetes risk. Further insight into the assembly of the metal centres in the cytosolic domain was gained from potentiometric investigations of zinc binding to synthetic peptides corresponding to N-terminal and C-terminal sequences of ZnT8 bearing the metal-coordinating ligands. Our work suggests the involvement of the C-terminal cysteines, which are part of the cytosolic domain, in a metal chelation and/or acquisition mechanism and, as now supported by the high-resolution structural work, provides the first example of metal-thiolate coordination chemistry in zinc transporters.
The Impact of Modern Technologies on Molecular Diagnostic Success Rates, with a Focus on Inherited Retinal Dystrophy and Hearing Loss
Authors: de Bruijn Suzanne E. , Fadaie Zeinab , Cremers Frans P. M. , Kremer Hannie , Roosing Susanne
Published: March 2021

The identification of pathogenic variants in monogenic diseases has been of interest to researchers and clinicians for several decades. However, for inherited diseases with extremely high genetic heterogeneity, such as hearing loss and retinal dystrophies, establishing a molecular diagnosis requires an enormous effort. In this review, we use these two genetic conditions as examples to describe the initial molecular genetic identification approaches, as performed since the early 90s, and subsequent improvements and refinements introduced over the years. Next, the history of DNA sequencing from conventional Sanger sequencing to high-throughput massive parallel sequencing, a.k.a. next-generation sequencing, is outlined, including their advantages and limitations and their impact on identifying the remaining genetic defects. Moreover, the development of recent technologies, also coined “third-generation” sequencing, is reviewed, which holds the promise to overcome these limitations. Furthermore, we outline the importance and complexity of variant interpretation in clinical diagnostic settings concerning the massive number of different variants identified by these methods. Finally, we briefly mention the development of novel approaches such as optical mapping and multiomics, which can help to further identify genetic defects in the near future.