bluuebunny/crossref_metadata_2025 huggingface dataset:
Circadian rhythm of PERIOD2::LUCIFERASE expression in the trigeminal ganglion of mice
Authors: Shirakawa Yukie , Ohno Sachi N. , Yamagata Kanae A. , Kuramoto Eriko , Oda Yoshiaki , Nakamura Takahiro J. , Nakamura Wataru , Sugimura Mitsutaka
Published: March 2023

Introduction

The trigeminal nerve conveys delicate sensations such as warmth, pain, and tactile pressure in the oral and facial regions, and most trigeminal afferent cell bodies are located in the trigeminal ganglion. Our previous study has shown that sensations in trigeminal nerve innervated areas, specifically in the maxillofacial region, exhibit diurnal variation and that sensitivity changes time-dependently. In this study, we aimed to clarify the rhythm of expression of clock gene in the trigeminal ganglion of mice to elucidate the mechanism of circadian regulation in the same area.

Methods

Immunohistochemistry examined the expression of the PER2 protein in the suprachiasmatic nucleus and trigeminal ganglion of wild-type mice. To measure gene expression as bioluminescence, PERIOD2::LUCIFERASE knock-in (PER2::LUC) mice were used. Unilateral trigeminal ganglion and brain sections including the suprachiasmatic nucleus were incubated ex vivo. Bioluminescence levels were then measured using a highly sensitive photodetector. The same experiments were then conducted with Cry1 gene-deficient (Cry1−/−) or Cry2 gene-deficient (Cry2−/−) mice.

Results

In the trigeminal ganglion, immunohistochemistry localized PER2 protein expression within the neuronal cell body. Mouse trigeminal ganglion ex vivo tissues showed distinct circadian oscillations in PER2::LUC levels in all genotypes, wild-type, Cry1−/−, and Cry2−/−. The period was shorter in the trigeminal ganglion than in the suprachiasmatic nucleus; it was shorter in Cry1−/− and longer in Cry2−/− mice than in the wild-type mice.

Conclusion

The expression of Per2 in neurons of the trigeminal ganglion in ex vivo culture and the oscillation in a distinct circadian rhythm suggests that the trigeminal ganglion is responsible for the relay of sensory inputs and temporal gating through autonomous circadian oscillations.
Case report: Resolution of malignant canine mast cell tumor using ketogenic metabolic therapy alone
Authors: Seyfried Thomas N. , Mukherjee Purna , Lee Derek C. , Ta Linh , Nations Loren
Published: March 2023

Background

Mast cell tumors (MCT) are common neoplasms in dogs and are similar to most other malignant cancers in requiring glucose for growth, regardless of histological grade. Ketogenic metabolic therapy (KMT) is emerging as a non-toxic nutritional intervention for cancer management in animals and humans alike. We report the case of a 7 years-old Pit Bull terrier that presented in 2011 with a cutaneous mast cell tumor under the right nostril.

Methods

The patient’s parent refused standard of care (SOC) and steroid medication after initial tumor diagnosis due to the unacceptable adverse effects of these treatments. Following tumor diagnosis, the patient’s diet was switched from Ol’Roy dog food to raw vegetables with cooked fish. The tumor continued to grow on this diet until July, 2013 when the diet was switched to a carbohydrate free, raw calorie restricted ketogenic diet consisting mostly of chicken and oils. A dog food calculator was used to reduce calories to 60% (40% calorie restriction) of that consumed on the original diet. A total of 444 kilocalories were given twice/day at 12 h intervals with one medium-sized raw radish given as a treat between each meal.

Results

The tumor grew to about 3–4 cm and invaded surrounding tissues while the patient was on the raw vegetable, cooked fish diet. The tumor gradually disappeared over a period of several months when the patient was switched to the carbohydrate free calorie restricted ketogenic diet. The patient lost 2.5 kg during the course of the calorie restriction and maintained an attentive and active behavior. The patient passed away without pain on June 4, 2019 (age 15 years) from failure to thrive due to an enlarged heart with no evidence of mast cell tumor recurrence.

Conclusion

This is the first report of a malignant cutaneous mast cell tumor in a dog treated with KMT alone. The resolution of the tumor in this canine patient could have been due to the diet-induced energy stress and the restriction of glucose-driven aerobic fermentation that is essential for the growth of most malignant tumors. Further studies are needed to determine if this non-toxic dietary therapeutic strategy could be effective in managing other canine patients with malignant mast cell tumors.
QE evolution of bialkali photocathode at cryogenic temperature
Authors: Zhao Yonglong , Ouyang Dongming , Xie Huamu , Liu Kexin , Huang Senlin
Published: March 2023

Photocathode plays an important role in generating high brightness and low emittance electron beam and K-Cs-Sb photocathode is preferred in SRF injectors for its high quantum efficiency, long lifetime, and low thermal emittance. To predict the QE change of K-Cs-Sb at cryogenic temperature more correctly, we have modified the QE formula of Spicer’s photoemission model considering the temperature dependence of work function and mean free path of photocathode material, which is obtained by fitting experimental data. The calculated QE drop of about 90.1% at 36.4 K fits well with the measured QE change in DC-SRF photoinjector at Peking University. This improved formula could provide a method to evaluate the QE performance of semiconductor photocathodes at cryogenic temperature.
Dual thick and thin filament linked regulation of stretch- and L-NAME-induced tone in young and senescent murine basilar artery
Authors: Lubomirov Lubomir T. , Schroeter Mechthild M. , Hasse Veronika , Frohn Marina , Metzler Doris , Bust Maria , Pryymachuk Galyna , Hescheler Jürgen , Grisk Olaf , Chalovich Joseph M. , Smyth Neil R. , Pfitzer Gabriele , Papadopoulos Symeon
Published: March 2023

Stretch-induced vascular tone is an important element of autoregulatory adaptation of cerebral vasculature to maintain cerebral flow constant despite changes in perfusion pressure. Little is known as to the regulation of tone in senescent basilar arteries. We tested the hypothesis, that thin filament mechanisms in addition to smooth muscle myosin-II regulatory-light-chain-(MLC20)-phosphorylation and non-muscle-myosin-II, contribute to regulation of stretch-induced tone. In young BAs (y-BAs) mechanical stretch does not lead to spontaneous tone generation. Stretch-induced tone in y-BAs appeared only after inhibition of NO-release by L-NAME and was fully prevented by treatment with 3 μmol/L RhoA-kinase (ROK) inhibitor Y27632. L-NAME-induced tone was reduced in y-BAs from heterozygous mice carrying a point mutation of the targeting-subunit of the myosin phosphatase, MYPT1 at threonine696 (MYPT1-T696A/+). In y-BAs, MYPT1-T696A-mutation also blunted the ability of L-NAME to increase MLC20-phosphorylation. In contrast, senescent BAs (s-BAs; >24 months) developed stable spontaneous stretch-induced tone and pharmacological inhibition of NO-release by L-NAME led to an additive effect. In s-BAs the MYPT1-T696A mutation also blunted MLC20-phosphorylation, but did not prevent development of stretch-induced tone. In s-BAs from both lines, Y27632 completely abolished stretch- and L-NAME-induced tone. In s-BAs phosphorylation of non-muscle-myosin-S1943 and PAK1-T423, shown to be down-stream effectors of ROK was also reduced by Y27632 treatment. Stretch- and L-NAME tone were inhibited by inhibition of non-muscle myosin (NM-myosin) by blebbistatin. We also tested whether the substrate of PAK1 the thin-filament associated protein, caldesmon is involved in the regulation of stretch-induced tone in advanced age. BAs obtained from heterozygotes Cald1+/− mice generated stretch-induced tone already at an age of 20–21 months old BAs (o-BA). The magnitude of stretch-induced tone in Cald1+/− o-BAs was similar to that in s-BA. In addition, truncation of caldesmon myosin binding Exon2 (CaD-▵Ex2−/−) did not accelerate stretch-induced tone. Our study indicates that in senescent cerebral vessels, mechanisms distinct from MLC20 phosphorylation contribute to regulation of tone in the absence of a contractile agonist. While in y-and o-BA the canonical pathways, i.e., inhibition of MLCP by ROK and increase in pMLC20, predominate, tone regulation in senescence involves ROK regulated mechanisms, involving non-muscle-myosin and thin filament linked mechanisms involving caldesmon.
Site-directed mutagenesis identified the key active site residues of 2,3-oxidosqualene cyclase HcOSC6 responsible for cucurbitacins biosynthesis in Hemsleya chinensis
Authors: Li Xia , Chen Geng , Gao Qing-Qing , Xiang Chun-Fan , Yuan Cheng-Xiao , Li Xiao-Ning , Shu Yan-Yu , Zhang Guang-Hui , Liang Yan-Li , Yang Sheng-Chao , Zhai Chen-Xi , Zhao Yan
Published: March 2023

Hemsleya chinensis is a Chinese traditional medicinal plant, containing cucurbitacin IIa (CuIIa) and cucurbitacin IIb (CuIIb), both of which have a wide range of pharmacological effects, including antiallergic, anti-inflammatory, and anticancer properties. However, few studies have been explored on the key enzymes that are involved in cucurbitacins biosynthesis in H. chinensis. Oxidosqualene cyclase (OSC) is a vital enzyme for cyclizing 2,3-oxidosqualene and its analogues. Here, a gene encoding the oxidosqualene cyclase of H. chinensis (HcOSC6), catalyzing to produce cucurbitadienol, was used as a template of mutagenesis. With the assistance of AlphaFold2 and molecular docking, we have proposed for the first time to our knowledge the 3D structure of HcOSC6 and its binding features to 2,3-oxidosqualene. Mutagenesis experiments on HcOSC6 generated seventeen different single-point mutants, showing that single-residue changes could affect its activity. Three key amino acid residues of HcOSC6, E246, M261 and D490, were identified as a prominent role in controlling cyclization ability. Our findings not only comprehensively characterize three key residues that are potentially useful for producing cucurbitacins, but also provide insights into the significant role they could play in metabolic engineering.
Identification of CaPs locus involving in purple stripe formation on unripe fruit, reveals allelic variation and alternative splicing of R2R3-MYB transcription factor in pepper (Capsicum annuum L.)
Authors: Li Ning , Liu Yabo , Yin Yanxu , Gao Shenghua , Wu Fangyuan , Yu Chuying , Wang Fei , Kang Byoung−Cheorl , Xu Kai , Jiao Chunhai , Yao Minghua
Published: March 2023

The purple color of unripe pepper fruit is attributed to the accumulation of anthocyanins. Only a few genes controlling the biosynthesis and regulation of anthocyanins have been cloned in Capsicum. In this study, we performed a bulked segregant analysis of the purple striped trait using an F2 population derived from a cross between the immature purple striped fruit line Chen12-4-1-1-1-1 and the normal green fruit line Zhongxian101-M-F9. We mapped the CaPs locus to an 841.39 kb region between markers M-CA690-Xba and MCA710-03 on chromosome 10. CA10g11690 encodes an R2R3-MYB transcription factor that is involved in the biosynthesis of anthocyanins as the best candidate gene. Overexpression and silencing in transformed tobacco (Nicotiana tabacum) lines indicated that CA10g11690 is involved in the formation of purple stripes in the exocarp. A comparison of parental sequences identified an insertion fragment of 1,926 bp in the second intron region of Chen12-4, and eight SNPs were detected between the two parents. Additionally, there were 49 single nucleotide polymorphic variations, two sequence deletions, and four sequence insertions in the promoter region. We found that CA10g11690 undergoes alternative splicing and generates different transcripts. Thus, the functional transcript of CA10g11690 appeared to be primarily involved in the development of purple phenotype in the exocarp. Our data provide new insight into the mechanism of anthocyanin biosynthesis and a theoretical basis for the future breeding of purple striped pepper varieties.
Evaluation of the growth-inducing efficacy of various Bacillus species on the salt-stressed tomato (Lycopersicon esculentum Mill.)
Authors: Patani Anil , Prajapati Dharmendra , Ali Daoud , Kalasariya Haresh , Yadav Virendra Kumar , Tank Jigna , Bagatharia Snehal , Joshi Madhvi , Patel Ashish
Published: March 2023

Plants are affected by salt stress in a variety of ways, including water deficiency, ion toxicity, nutrient imbalance, and oxidative stress, all of which can cause cellular damage or plant death. Halotolerant plant growth-promoting rhizobacteria (PGPR) could be a viable alternative for tomato plants growing in arid and semi-arid environments. The aim of this research was to isolate halotolerant plant growth promoting Bacillus sp. to promote tomato (Lycopersicon esculentum Mill.) growth and salt stress resistance. 107 PGPR strains were isolated from the rhizospheres of ‘Kesudo’ (Butea monosperma Lam.), ‘Kawaria’ (Cassia tora L.), and ‘Arjun’ (Terminalia arjuna Roxb.) plants to test their plant growth promoting abilities, including indole-3-acetic acid, phosphate solubilization, siderophore production, and ACC deaminase activity. Five bacterial strains (Bacillus pumilus (NCT4), Bacillus firmus (NCT1), Bacillus licheniformis (LCT4), Bacillus cereus (LAT3), and Bacillus safensis (LBM4)) were chosen for 16S rRNA on the basis of PGPR traits. Compared to PGPR untreated plants, tomato plants developed from PGPR-treated seeds had considerably increased germination percentage, seedling growth, plant height, dry weight, and leaf area. As comparison to PGPR non-inoculated plants, salt-stressed tomato plants treated with PGPR strains had higher levels of total soluble sugar, proline, and chlorophyll as well as higher levels of SOD, CAT, APX, and GR activity. PGPR-inoculated salt-stressed tomato plants had lower MDA, sodium, and chloride levels than non-inoculated plants. In addition, magnesium, calcium, potassium, phosphorus, and iron levels were higher in PGPR treated plants when subjected to salt stress. These results indicate that halotolerant PGPR strains can increase tomato productivity and tolerance to salt stress by removing salt stress’s negative effects on plant growth.
Conceptual and methodological issues in the study of the personality-and-culture relationship
Authors: Allik Jüri , Realo Anu , McCrae Robert R.
Published: March 2023

Culture-and-personality studies were central to social science in the early 20th century and have recently been revived (as personality-and-culture studies) by trait and cross-cultural psychologists. In this article we comment on conceptual issues, including the nature of traits and the nature of the personality-and-culture relationship, and we describe methodological challenges in understanding associations between features of culture and aspects of personality. We give an overview of research hypothesizing the shaping of personality traits by culture, reviewing studies of indigenous traits, acculturation and sojourner effects, birth cohorts, social role changes, and ideological interventions. We also consider the possibility that aggregate traits affect culture, through psychological means and gene flow. In all these cases we highlight alternative explanations and the need for designs and analyses that strengthen the interpretation of observations. We offer a set of testable hypotheses based on the premises that personality is adequately described by Five-Factor Theory, and that observed differences in aggregate personality traits across cultures are veridical. It is clear that culture has dramatic effects on the thoughts, feelings, and behaviors from which we infer traits, but it is not yet clear whether, how, and in what degree culture shapes traits themselves.
Transmedia storytelling usage of neural networks from a Universal Design for Learning perspective: A systematic review
Authors: Meyerhofer-Parra Rafel , González-Martínez Juan
Published: March 2023

The use of transmedia storytelling (TST) experiences is increasingly common in today's media ecology. Mediated by participatory culture, the role of the prosumer, and competency processes that connect with the reality of learners, the incorporation of storytelling motivates and deploys diverse didactic strategies. Considering the engagement generated by these strategies, and the need to promote literacies to provide competences to a plural society, a systematic review of the literature on transmedia storytelling experiences from the perspective of universal design for learning (UD-L) using PRISMA is carried out: a priori, we start from the idea that, if UD-L is based on the principles of educational neuroscience and TST, in turn, concretizes some of the guidelines of UD-L, TST can naturally result in a didactic approach that capitalizes on educational neuroscientific knowledge in a harmonious way with the digital context in which we live. The review analyzes a total of 50 articles from four databases: ERIC, Scopus, Web of Science, and Dialnet. The results show a low development of the checkpoints of the UD-L guides, and it is concluded that the most worked checkpoints are those closest to the definition of transmedia storytelling, followed by the foundational aspects of UD-L and, finally, aspects of access. Engagement is reflected in the experiences, but scaffolding is required to consolidate learning. In addition to this is the need to guarantee a true participatory culture, which requires the integration of more elements that incorporate accessibility into didactic strategies, offering learning possibilities for different styles and forms.
Gender-moderated effects of academic self-concept on achievement, motivation, performance, and self-efficacy: A systematic review
Authors: Wang Leihong , Yu Zhonggen
Published: March 2023

Although academic self-concept plays a crucial role in promoting students' education, there is a paucity of studies simultaneously exploring the gender-moderated effects of academic self-concept. This study aimed to explore gender-moderated effects of academic self-concept on achievement, motivation, performance, and self-efficacy. With Preferred Reporting Items for Systematic Reviews and Meta-Analyses Protocol (PRISMA-P) and STARLITE criteria, this study screened and assessed the retrieved literature, finally including 53 studies. It was concluded that academic self-concept exerted a positive influence on improving achievement, enhancing motivation, ameliorating performance, and boosting self-efficacy. It should also be noted that interrelations between academic self-concept and other educational constructs may be much more complicated than expected since gender disparities may moderate the effects of academic self-concept. Gender discrepancies in academic self-concept could account for the gap between male students and female students in subject-specific achievement, motivation, performance, and self-efficacy, especially in STEM courses. Teaching interventions and educational policies should be taken to enhance female students' STEM courses self-concept. Future studies should promote educational equality, highlight academic self-concept of special groups, and enhance academic self-concept in online learning.

Systematic review registration

https://osf.io/uxjnv/?view_only=b10db44d34154d96a361c159ca15a5b5.
Examining mindfulness and moral disengagement in doping: Perspective of Turkish wrestlers
Authors: Ayyildiz Erdem , Kayabeşler Hamza , Gülü Mehmet , Yagin Fatma Hilal , Aldhahi Monira I. , García-Grimau Elena , Al-Mhanna Sameer Badri
Published: March 2023

Introduction

Studies related to attitudes toward the use of prohibited substances in Turkish athletes are scarce. The World Anti-Doping Agency (WADA) has implemented anti-doping educational policies emphasizing doping-related education in studies conducted among Turkish wrestlers. However, it is still unclear the extent to which the wrestlers comply and adhere to these anti-doping policies. No research has previously examined the effect of anti-doping education on athletes' mindfulness and moral disengagement in doping (MDD). Therefore, the present study has a two-fold objective: first, to examine whether doping-related education (DRE) and the status of being a national athlete (NA) have an effect on athlete mindfulness and MDD. Second, to analyze the relationship between each sub-dimensions of athlete mindfulness: awareness (ASD), judgment (JSD), and refocus (RSD) with MDD.

Methods

A total of 409 male wrestlers participated in this study. MANOVA analysis showed that NA and DRE alone have no effect on MDD but have a general effect on mindfulness.

Results

The highest effect was on the ASD of being an NA (ηp2 = 0.173). When the interaction effect of NA*DRE was examined, significant difference in MDD (F = 8.218, p = 0.004), ASD (F = 8.476, p = 0.004), JSD (F = 5.844, p = 0.016), and RSD (F = 11.476, p = 0.001) were found. MDD has a weak negative relationship with ASD (r = −0.126) and RSD (r = −0.041) and a weak positive relationship with the JSD sub-dimension (r = 0.140). Those results suggest that being a NA and having received anti-doping education affect moral disengagement in doping and athletes' mindfulness.

Discussion

As a conclusion, it is recommended to increase awareness and anti-doping education among national-standard Turkish wrestlers to prevent them from engaging in doping behaviors.
The central role of symptom severity and associated characteristics for functional impairment in misophonia
Authors: Möllmann Anne , Heinrichs Nina , Illies Lisa , Potthast Nadine , Kley Hanna
Published: March 2023

Misophonia is characterized by a preoccupation with and strong emotional and behavioral reactions to certain triggers, mostly sounds related to eating and breathing. We applied functional impairment due to misophonic symptoms as a central criterion to investigate differences between clinical misophonia and normative decreased sound tolerance in a large non-random sample of n = 1,881 individuals from an online survey. We assessed the frequency of self-reported misophonia symptoms across various symptom measures, compared severity, triggers and emotional reactions, general psychopathology, interpersonal emotion regulation, and quality of life between both groups with and without functional impairing misophonia. Individuals with functional impairment due to misophonia (n = 839) revealed significantly higher general psychopathology symptoms, lower interpersonal emotion regulation skills, and lower quality of life than individuals without impairment (n = 1,042). Anxious/distressed and annoyed reactions to triggers were experienced more frequently compared to emotional reactions of disgust and sadness or depression in both groups. Overall, the group differences were primarily quantitative in nature. We discuss practical implications regarding classification and treatment and provide cutoffs for each symptom measure derived from group assignment for functional impairment.
Planned adaptation and implementation of the Community Guide recommendations for increasing physical activity in rural community settings: A qualitative study
Authors: Hess Julia Meredith , Davis Sally M.
Published: March 2023

Background

The purpose of this paper is to report on the implementation of an evidence-based model, VIVA, which was developed to translate physical activity (PA) recommendations to rural environments and was scaled-up to 12 rural communities across New Mexico. Our longitudinal qualitative research describes processes of planned adaptation in the rural context with an exploration of inner and outer context adaptations that consider important implementation constructs including leadership, partnership and collaboration.

Materials & methods

An enhanced version of the RE-AIM framework was used to formulate community-level engagement and process questions essential to implementation science. Qualitative methods, using a thematic approach that included both inductive and deductive coding with attention to processes, was used to explore adaptation at the community level. Data included semi-structured interviews with 17 community leaders at baseline and 10 at follow-up, fieldnotes, and technical assistance tracking forms. Analysis was conducted with NVivo qualitative data analysis software.

Results

Analysis demonstrated how planned adaptation of the implementation model was critical to dissemination in rural communities. Understanding and adapting to local context—including geography, culture, economics—is essential for implementation. Inner context constructs, recognized as important across implementation models, including leadership, partnerships and political engagement were found to be key to implementation success. Moreover, we provide concrete examples of the range and complexity of these issues in rural communities, and how these shaped implementation uptake and success.

Discussion

Studying processes of planned adaptation in rural contexts will further implementation science efforts to move evidence into practice. It is essential to incorporate planned adaptation to local, community contexts to create models which are simple to encourage adoption, are evidence-based, and are adaptable to local conditions without compromising the integrity of the evidence-based model.
Global, regional, and national burden of 10 digestive diseases in 204 countries and territories from 1990 to 2019
Authors: Wang Rui , Li Zhaoqi , Liu Shaojun , Zhang Decai
Published: March 2023

Background

Digestive diseases are very common worldwide and account for considerable health care use and expenditures. However, there are no global population-based estimates of the disease burden and temporal trend of digestive diseases.

Methods

Annual case numbers, age-standardized rates of prevalence, incidence, death, and disability-adjusted life-years (DALYs), and their estimated annual percentage changes (EAPCs) for digestive diseases between 1990 and 2019 were derived from the Global Burden of Disease, Injuries, and Risk Factors Study (GBD) 2019. The association between digestive disease burden and the sociodemographic index (SDI) was investigated. We also calculated DALYs attributable to risk factors that had evidence of causation with digestive diseases.

Results

Globally, in 2019, there were 88.99 million DALYs due to digestive diseases (3.51% of global DALYs). Digestive diseases were the 13th leading cause of DALYs globally in 2019. Global digestive disease DALYs were highest in the middle SDI quintile and in South Asia and were higher in males than females in 2019. Cirrhosis and other chronic liver diseases constituted the highest proportion of categorized digestive disease DALY burdens globally. From 1990 to 2019, the global age-standardized DALY rate of digestive diseases decreased from 1570.35 in 1990 to 1096.99 in 2019 per 1,00,000 population, with the EAPC being −1.32 (95% confidence interval [CI] −1.36 to −1.27). In 2019, the largest contributor to digestive disease DALYs at the global level, for both sexes, was alcohol use.

Conclusion

The results of this systematic analysis suggest that the global burden of digestive diseases is substantial and varies markedly according to age, sex, SDI, and geographical region. These results provide comprehensive and comparable estimates that can potentially inform efforts toward digestive disease control worldwide.
Long-term exposure to particulate matter on cardiovascular and respiratory diseases in low- and middle-income countries: A systematic review and meta-analysis
Authors: Guo Juanmei , Chai Guorong , Song Xuping , Hui Xu , Li Zhihong , Feng Xiaowen , Yang Kehu
Published: March 2023

Background

Long-term exposure to particulate matter (PM) has essential and profound effects on human health, but most current studies focus on high-income countries. Evidence of the correlations between PM and health effects in low- and middle-income countries (LMICs), especially the risk factor PM1 (particles < 1 μm in size), remains unclear.

Objective

To explore the effects of long-term exposure to particulate matter on the morbidity and mortality of cardiovascular and respiratory diseases in LMICs.

Methods

A systematic search was conducted in the PubMed, Web of Science, and Embase databases from inception to May 1, 2022. Cohort studies and case-control studies that examine the effects of PM1, PM2.5, and PM10 on the morbidity and mortality of cardiovascular and respiratory diseases in LMICs were included. Two reviewers independently selected the studies, extracted the data, and assessed the risk of bias. Outcomes were analyzed via a random effects model and are reported as the relative risk (RR) with 95% CI.

Results

Of the 1,978 studies that were identified, 38 met all the eligibility criteria. The studies indicated that long-term exposure to PM2.5, PM10, and PM1 was associated with cardiovascular and respiratory diseases: (1) Long-term exposure to PM2.5 was associated with an increased risk of cardiovascular morbidity (RR per 1.11 μg/m3, 95% CI: 1.05, 1.17) and mortality (RR per 1.10 μg/m3, 95% CI: 1.06, 1.14) and was significantly associated with respiratory mortality (RR 1.31, 95% CI: 1.25, 1.38) and morbidity (RR 1.08, 95% CI: 1.02, 1.04); (2) An increased risk of respiratory mortality was observed in the elderly (65+ years) (RR 1.21, 95% CI: 1.00, 1.47) with long-term exposure to PM2.5; (3) Long-term exposure to PM10 was associated with cardiovascular morbidity (RR 1.07, 95% CI 1.01, 1.13), respiratory morbidity (RR 1.43, 95% CI: 1.21, 1.69) and respiratory mortality (RR 1.28, 95% CI 1.10, 1.49); (4) A significant association between long-term exposure to PM1 and cardiovascular disease was also observed.

Conclusions

Long-term exposure to PM2.5, PM10 and PM1 was all related to cardiovascular and respiratory disease events. PM2.5 had a greater effect than PM10, especially on respiratory diseases, and the risk of respiratory mortality was significantly higher for LMICs than high-income countries. More studies are needed to confirm the effect of PM1 on cardiovascular and respiratory diseases.
Supplementation of feed and water after long-duration road transportation: The effects on welfare and rumen fermentation in goats
Authors: Xu Ke , Yang Kang , Yang Yi , Wu Wenxuan , Zhou Chuanshe
Published: March 2023

Water and feed are needed for livestock during their long-duration road transportation. However, limited information is available on the need to supply water and feed to livestock at temporary holding stations after road transportation. This study aimed to evaluate the effect of providing water and feed at holding stations on the welfare of goats in mimic surroundings. A total of 24 Guizhou black goats were randomly divided into three groups of eight goats each as follows: deprived of water and feed (TRT0), supplemented with water ad libitum (TRT1), and supplemented with water and feed ad libitum (TRT2). Blood and rumen fluid samples were collected before loading (denoted as “PRE” in this article) and after transport (denoted as “POST” in this article). Statistical analysis was performed via the SAS procedure PROC MIXED. The 10-h road transportation period reduced body weight in TRT0 goats (p < 0.05) but not in TRT1 and TRT2 (p > 0.05). TRT0 and TRT1 goats had POST plasma glucose concentrations above their PRE values (p < 0.05). The PRE-plasma urea nitrogen (PUN) levels were higher in TRT2 compared to TRT0 (p < 0.05) goats, while the POST–PUN levels increased in TRT1 compared to TRT0 goats. The POST non-esterified fatty acid (NEFA) concentration was higher for TRT0 compared to that in TRT1 and TRT2 (p < 0.05) goats. No difference was observed for plasma profiles of malondialdehyde (MDA) and superoxide dismutase (SOD) (p > 0.05). TRT2 goats had higher POST glutathione peroxidase (GSH-Px) activity than TRT0 and TRT1 (p < 0.05) goats. TRT0 goats had higher POST plasma glucagon (GC) compared to TRT2 (p < 0.05) and had increased values compared to their own PRE level as a result of road transportation (p < 0.05). TRT2 goats resulted in a lower POST plasma heat-stressed protein-70 (HSP-70) level than TRT0. There was no difference in ruminal pH (p > 0.05). Ruminal total VFA (acetate, propionate, butyrate), and the NH3-ammonia profiles showed a decrease (p < 0.05) after transportation in all groups. Ruminal microcrystalline cellulose, xylanase, cellobiase, and carboxymethyl cellulose activities were unaffected (p > 0.05). These combined results imply that water and feed supplementation to livestock can effectively alleviate stress responses in goats subjected to road transportation and emphasize the necessity to establish water and feed supplies even at a temporary holding pen.
Variation of existence and location of aquaporin 3 in relation to cryoresistance of ram spermatozoa
Authors: Pequeño Belén , Castaño Cristina , Alvarez-Rodriguez Manuel , Bóveda Paula , Millán de la Blanca María Gemma , Toledano-Díaz Adolfo , Galarza Diego Andres , Rodriguez-Martinez Heriberto , Martínez-Madrid Belén , Santiago-Moreno Julián
Published: March 2023

Introduction and objective

Osmotic changes during the process of freeze-thawing involve changes in the location of aquaporins (AQPs) in membrane domains of spermatozoa. Some AQPs, like aquaporin 3 (AQP3), are linked to sperm cryotolerance in the porcine species. Conspicuous individual variability exists between rams and their ejaculates, which may be classified as displaying good freezability (GFE) or poor freezability (PFE), depending on several endogenous and environmental factors. The present work aimed to examine whether differences in freezability could even involve changes in location and expression of AQP3 in ram spermatozoa.

Methods

Thirty ejaculates from 10 rams (three of each) were evaluated and subsequently classified as GFE (n= 13) or PFE (n= 17) through a principal component analysis (PCA) and k-means cluster analysis. Spermatozoa were examined for the presence, abundance and distribution of AQP3 by western blot and immunocytochemistry, employing a commercial rabbit polyclonal antibody (AQP3 - ab125219).

Results and discussion

Although AQP3 was found in the sperm acrosome, midpiece, principal and end piece of the tail in both fresh and after frozen-thawed samples, its highest immunolabeling was found in the mid- and principal piece. In the GFE group, the expression of AQP3 in the mid- and principal piece was greater (P< 0.05) in frozen-thawed samples than in fresh specimens while such differences were not detected in the PFE group. Sperm cryotolerance relates to changes in AQP3 expression and thus AQP3 could be used as a biomarker for cryotolerance.

Conclusion

A greater capacity of AQP3 localization in mid- and principal piece of the spermatozoa could be linked to an increase the osmo-adaptative capacity of ejaculates with better capacity to withstand freeze-thawing processes.
Effect of Ecologically Restored Vegetation Roots on the Stability of Shallow Aggregates in Ionic Rare Earth Tailings Piles
Authors: Zhong Wen , Shuai Qi , Zeng Peng , Guo Zhongqun , Hu Kaijian , Wang Xiaojun , Zeng Fangjin , Zhu Jianxin , Feng Xiao , Lin Shengjie , Feng Zhiqi
Published: March 2023

Aggregate stability is considered an essential indicator of changes in the physical properties of soils, and vegetation roots play a crucial role in the stability of shallow soil aggregates in ionic rare earth tailings piles during ecological remediation. In this paper, the influence of the law of ecologically restored vegetation roots on the stability of shallow aggregates of ionic rare earth tailing piles was investigated by means of field investigation tests, indoor experiments and mathematical statistics. The influence of different types of root systems on the stability of the shallow depth range aggregates of tailings piles was investigated; the correlation between vegetation root systems and the main physical parameters of rare earth tailings was clarified; and a mathematical correlation model characterizing the characteristic parameters of vegetation root systems was constructed. The evaluation index of the stability of rare earth tailings piles was constructed, and the influence of the law of the ecological restoration of vegetation root systems on the strength of shallow aggregates of ionic rare earth tailings piles was revealed. The results of the study showed that compared with the RD (root density), the root characteristic parameter with the largest response weight to the rare earth tailings pile is the RL (root length density), and the root characteristic parameter with the largest response weight to the water content is the RV (root volume). Suitable vegetation roots can effectively enhance the content of shallow large aggregates of rare ionic earth tailing piles. With the increase of the depth of a tailing pile, the content of large aggregates continues to decrease, and the content of micro aggregates continues increasing. This indicates that the vegetation root system changed the shallow soil of the rare earth tailing pile from disorderly to orderly through its own growth pattern, which effectively improved the stability of the shallow aggregates of the tailing pile and improved the physical properties of the tailing.
Impact of COVID-19 Pandemic on Antibiotic Utilisation in Malaysian Primary Care Clinics: An Interrupted Time Series Analysis
Authors: Lim Audrey Huili , Ab Rahman Norazida , Hashim Hazimah , Kamal Mardhiyah , Velvanathan Tineshwaran , Chok Mary Chiew Fong , Sivasampu Sheamini
Published: March 2023

The COVID-19 pandemic has resulted in a dramatic change in the delivery of primary healthcare across the world, presumably changing trends in consultations for infectious diseases and antibiotic use. This study aimed at describing and evaluating the impact of COVID-19 on antibiotic use in public primary care clinics in Malaysia between 2018 and 2021. Data from the nationwide procurement database of systemic antibiotics from public primary care clinics in Malaysia between January 2018 and December 2021 were analysed using interrupted time series analysis. The monthly number of defined daily doses per 1000 inhabitants per day (DID) was calculated and grouped by antibiotic class. The trend of antibiotic utilisation rates had been decreasing by 0.007 DID monthly before March 2020 (p = 0.659). With the introduction of national lockdown due to COVID-19 beginning March 2020, there was a significant reduction in the level of antibiotic utilisation rates of 0.707 (p = 0.022). Subsequently, the monthly trend showed a slight upward change until the end of the study period (p = 0.583). Our findings indicate that there was a significant decrease in antibiotic utilisation for systemic use in primary care following the COVID-19 pandemic compared with the preceding years (January 2018–March 2020).
The Nrf2 Pathway in Depressive Disorders: A Systematic Review of Animal and Human Studies
Authors: Sani Gabriele , Margoni Stella , Brugnami Andrea , Ferrara Ottavia Marianna , Bernardi Evelina , Simonetti Alessio , Monti Laura , Mazza Marianna , Janiri Delfina , Moccia Lorenzo , Kotzalidis Georgios D. , Chieffo Daniela Pia Rosaria , Janiri Luigi
Published: March 2023

There is increasing interest in the involvement of antioxidative systems in protecting from depression. Among these, Nrf2 occupies a central place. We aimed to review the role of Nrf2 in depression. For this reason, we conducted a PubMed search using as search strategy (psychiatr*[ti] OR schizo*[ti] OR psychot*[ti] OR psychos*[ti] OR depress*[ti] OR MDD[ti] OR BD[ti] OR bipolar[ti] OR Anxiety[ti] OR antidepress*[ti] OR panic[ti] OR obsess*[ti] OR compulsio*[ti] OR “mood disord*”[ti] OR phobi*[ti] OR agoraphob*[ti] OR anorex*[ti] OR anorect*[ti] OR bulimi*[ti] OR “eating disorder*”[ti] OR neurodevelopm*[ti] OR retardation[ti] OR autism[ti] OR autistic[ti] OR ASM[ti] OR adhd[ti] OR “attention-deficit”[ti]) AND nrf2, which on the 9th of March produced 208 results of which 89 were eligible for our purposes. Eligible articles were studies reporting data of Nrf2 manipulations or content by any treatment in human patients or animals with any animal model of depression. Most studies were on mice only (N = 58), 20 on rats only, and three on both rats and mice. There were two studies on cell lines (in vitro) and one each on nematodes and fish. Only four studies were conducted in humans, one of which was post mortem. Most studies were conducted on male animals; however, human studies were carried out on both men and women. The results indicate that Nrf2 is lower in depression and that antidepressant methods (drugs or other methods) increase it. Antioxidant systems and plasticity-promoting molecules, such as those in the Nrf2–HO-1, BDNF–TrkB, and cyclic AMP–CREB pathways, could protect from depression, while glycogen synthase kinase-3β and nuclear factor κB oppose these actions, thus increasing depressive-like behaviours. Since Nrf2 is also endowed with tumorigenic and atherogenic potential, the balance between benefits and harms must be taken into account in designing novel drugs aiming at increasing the intracellular content of Nrf2.
Properties of Selenolate-Diselenide Redox Equilibria in View of Their Thiolate-Disulfide Counterparts
Authors: Pálla Tamás , Mirzahosseini Arash , Noszál Béla
Published: March 2023

Selenium, the multifaceted redox agent, is characterized in terms of oxidation states, with emphasis on selenol and diselenide in proteinogenic compounds. Selenocysteine, selenocystine, selenocysteamine, and selenocystamine are depicted in view of their co-dependent, interfering acid-base, and redox properties. The pH-dependent, apparent (conditional), and pH-independent, highly specific, microscopic forms of the redox equilibrium constants are described. Experimental techniques and evaluation methods for the determination of the equilibrium and redox parameters are discussed, with a focus on nuclear magnetic resonance spectroscopy, which is the prime technique to observe selenium properties in organic compounds. The correlation between redox, acid-base, and NMR parameters is shown in diagrams and tables. The fairly accessible NMR and acid-base parameters are discussed to assess the predictive power of these methods to estimate the site-specific redox properties of selenium-containing moieties in large molecules.
The Association of Physical Activity and Sedentary Behavior with Maternal and Cord Blood Anti-Oxidative Capacity and HDL Functionality: Findings of DALI Study
Authors: Zafaranieh Saghi , Stadler Julia T. , Pammer Anja , Marsche Gunther , van Poppel Mireille N. M. , Desoye Gernot ,   DALI Core Investigator Group
Published: March 2023

Obesity is one of the most common health issues in pregnancy with short and long-term consequences for both mother and her offspring. Promoting moderate to vigorous physical activity (MVPA) and decreasing sedentary time (ST) could have a positive impact on weight and obesity management, and therefore adiposity-induced oxidative stress, inflammation, and atherogenesis. However, the effects of MVPA and ST on anti-oxidative and anti-atherogenic markers in pregnancy have not been studied to date. This study aimed to assess the association of longitudinally and objectively measured MVPA and ST in 122 overweight/obese women (BMI ≥ 29 kg/m2) with maternal and cord blood markers of oxidative stress measured by advanced oxidation protein products (AOPP), anti-oxidative capacity, as well as high-density lipoproteins (HDL) related paraoxonase-1 (PON-1) activity and cholesterol efflux. Linear regression models showed no associations of MVPA and ST with outcomes in maternal blood. In contrast, MVPA at <20 weeks and 24–28 weeks of gestation were positively associated with anti-oxidative capacity, as well as PON-1 activity of HDL in cord blood. MVPA at 35–37 weeks correlated with higher AOPP, as well as higher anti-oxidative capacity. ST <20 weeks was also positively associated with inhibition of oxidation in cord blood. We speculate that increasing MVPA of overweight/obese women during pregnancy attenuates the oxidative stress state in the new-born.
Flexible-Imaging-Fiber-Guided Intratracheal Intubation in Rodents
Authors: Jeong Sang Hoon , Kim Cherry , Lee Hong , Nam Yoon Jeong , Oh Chil hwan , Baek Yong-Wook , Lim Jungyun , Lee Ju-Han , Kim Jaeyoung
Published: March 2023

Although experiments on intratracheal intubation for animals are essential for research, it remains challenging. This study aimed to validate an animal model using a flexible imaging guide system that can be conveniently and safely used as a new method to provide easy access to organs in small animals. PBS (Phosphate Buffered Saline) and PHMG (Polyhexamethylene guanidine) were administered by intratracheal intubation to 20 rodents (10 mice and 10 rats), and the changes in the lungs were observed. Results were verified using lung tissue histopathologic staining through the intratracheally administered material, which confirmed that 100% of changes in lung tissue occurred in the PHMG-injected group, where intubation was facilitated using the flexible imaging guide. The drug was conveniently and safely administered. The flexible-imaging-fiber-guide-based intratracheal drug injectable system may be conveniently used by researchers. It allows drugs to be administered quantitatively, suggesting its potential wide use in drug development and toxicity evaluation.
Full-Field Vibration Measurements by Using High-Speed Two-Dimensional Digital Image Correlation
Authors: Lin Yuankun , Huang Pinbo , Ni Zihao , Xie Shengli , Bai Yulei , Dong Bo
Published: March 2023

This work developed a method that uses a single monochrome high-speed camera without sacrificing the spatial resolution to measure both in-plane and out-of-plane full-field vibrations. By using the high-speed camera and a two-dimensional digital image correlation (2D-DIC) algorithm, the method first extracts the out-of-plane displacement field from the measured virtual in-plane strains. Then it retrieves the in-plane displacement field after eliminating the out-of-plane motion-induced virtual component. For validation, in-plane and out-of-plane translation tests and single-frequency vibration experiments were carried out. The measurement results show good agreement with the reference values, indicating the effectiveness of the proposed high-speed 2D-DIC (HS-2D-DIC). Further, the natural frequencies and mode shapes of a rectangular cantilever panel were also measured successfully, exhibiting the method’s effectiveness in practical applications. Since the HS-2D-DIC requires only a single monochrome camera, no complex optical setup, and no complicated calibration process, the method can be developed as a competitive tool for full-field vibration characterizations.