bluuebunny/crossref_metadata_2025 huggingface dataset:
Flavobacterium frigoritolerans sp. nov. and Flavobacterium shii sp. nov., isolated from glaciers on the Tibetan Plateau
Authors: Yang Lei-Lei , Liu Hong-Can , Xin Yu-Hua , Liu Qing
Published: March 2023

The genus

Flavobacterium

belongs to the family

Flavobacteriaceae

and its members are widely distributed in the environment. Taxonomic descriptions of strains LS1R47T and LS1R49T isolated from the Laigu glacier on the Tibetan Plateau, China, are presented in this study. Both strains were psychrotolerant, Gram-stain-negative, aerobic and rod-shaped. The comparative analysis of 16S rRNA gene sequences showed that strain LS1R47T was closest to

Flavobacterium bizetiae

CIP 105534T (98.90 %) and strain LS1R49T was closest to

Flavobacterium collinsii

983-08T (98.73 %). The 16S rRNA gene sequence similarity, average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between the two novel isolates were 99.4, 86.0 and 30.9 %, respectively. The ANI and dDDH values between strains LS1R47T and LS1R49T and their closely relatives were below 87.6 and 33.3 %, respectively. Phylogenomic analysis showed that the two strains cluster together with

Flavobacterium hydatis

ATCC 29551T. Both strains contained MK-6 as sole quinone, phosphatidylethanolamine as the principal polar lipid, and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c), iso-C15 : 0 3-OH, C15 : 0 3-OH and iso-C17 : 0 3-OH as the main fatty acids. These results indicated that strains LS1R47T and LS1R49T represented two novel species within the genus

Flavobacterium

. Therefore, we propose two novel species, Flavobacterium frigoritolerans sp. nov. (LS1R47T=CGMCC 1.11577T=NBRC 113654T) and Flavobacterium shii sp. nov. (LS1R49T=CGMCC 1.11581T=NBRC 113652T).
Streptomyces longhuiensis sp. nov, a novel species isolated from soil of Lilium brownii in Hunan Province, PR China
Authors: Li Kaiqin , Man Yilong , Chen Xi , Liu Jia , Jiang Huidan , Liu Zheming , Ma Haihao , Zhu Hang , Zhou Yong , Zhang Chengjia , Zhou Xiaomao
Published: March 2023

An actinobacterium strain, designated BH-MK-02T, was isolated from the soil of Lilium brownii. The taxonomic position was determined using a polyphasic approach. Strain BH-MK-02T grew well on International

Streptomyces

Project series media and formed well-developed, branched substrate hyphae and aerial mycelium that differentiated into straight spore chains with a wrinkled surface. The diagnostic diamino acid was ll-diaminopimelic acid. The major menaquinones were MK-9(H4), MK-9(H6) and MK-9(H8). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannosides, phosphatidylglycerol and unidentified lipid spots. The predominant fatty acids were anteiso-C15 : 0, iso-C16 : 0, C16 : 0 and C16 : 1
 ω7c/C16 : 1
 ω6c. The phenotypic characteristics of strain BH-MK-02T indicated that it belonged to the genus

Streptomyces

. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain BH-MK-02T was most closely related to

Streptomyces aureus

CGMCC 4.1833T (99.7 %). However, the average nucleotide identity and digital DNA–DNA hybridization values between the whole-genome sequences of strain BH-MK-02T and

S. aureus

CGMCC 4.1833T were 78.1 and 23.2 %, respectively, below the 96.7 and 70 % cut-off points respectively recommended for delineating

Streptomyces

species. Furthermore, the novel isolate could be distinguished from

S. aureus

CGMCC 4.1833T by morphological, physiological and biochemical characteristics. Based on all these data, strain BH-MK-02T (=MCCC 1K06237T=JCM 34789T) clearly represents a novel species within the genus

Streptomyces

, for which the name Streptomyces longhuiensis sp. nov. is proposed.
Metabacillus arenae sp. nov., isolated from seashore sand
Authors: Fang Qingjian , Wu Qingjuan , Huang Huiqin , Hu Yonghua , Li Chenghua , Mo Kunlian
Published: March 2023

A Gram-stain-positive, non-motile, rod-shaped, facultatively anaerobic bacterium, designated as IB182487T, was isolated from a seashore sand sample collected from Zhaoshu Island, PR China. Strain IB182487T grew at pH 6.0–10.0 (optimum, pH 8.0), 4–45 °C (optimum, 25–30 °C) and with 0–17 % (w/v) NaCl (optimum, 2–10 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IB182487T belonged to the genus

Metabacillus

and was closely related to

Metabacillus idriensis

SMC 4352-2T, (96.6 %),

Metabacillus indicus

LMG 22858T (96.5 %),

Metabacillus niabensis

DSM 17723T (96.3 %) and

Metabacillus halosaccharovorans

DSM 25387T (96.1 %). Strain IB182487T had meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan and contained menaquinone MK-7 as the predominant isoprenoid quinone. Its polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids and three unidentified glycolipids. The major cellular fatty acids of strain IB182487T were iso-C15 : 0 and anteiso-C15 : 0. The whole genome average nucleotide identity and digital DNA–DNA hybridization analysis between the isolate and its closely related type strains demonstrated that the strain significantly differed from other

Metabacillus

species. The genomic DNA G+C content of strain IB182487T was 37.4 mol%. On the basis of phenotypic and chemotaxonomic properties, phylogenetic relatedness as well as genomic characteristics, strain IB182487T represents a novel species of the genus

Metabacillus

, for which the name Metabacillus arenae sp. nov. is proposed. The type strain of M. arenae is IB182487T (=MCCC 1K04629T=JCM 34523T).
Parapedobacter tibetensis sp. nov., isolated from shore soil of saline lake in Tibet of China and its genome mining of secondary metabolite
Authors: Wang Rui , Xue Lu , Liu Zi-Xuan , Li Dong-Yang , Liu Hong-Can , Li Ai-Hua , Zhou Yu-Guang
Published: March 2023

Five aerobic, Gram-stain-negative, non-motile, non-spore-forming, short rod bacteria strains, designated as C3-1-R+6T, C3-2-M9, B3-2-R-7, B3-2-R-21 and C3-2-M2, were isolated from shore soil of LungmuCo Lake in Tibet of China. The 16S rRNA gene sequence comparisons confirmed their affiliation to the genus

Parapedobacter

of the family

Sphingobacteriaceae

, and showed that they were most closely related to

Parapedobacter lycopersici

KACC 18788T with 94.26 % similarities. The average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA–DNA hybridization (dDDH) values between them and the validly published

Parapedobacter

species were all below the thresholds for delineating species, supporting that they were novel species of genus

Parapedobacter

. The ANI, AAI and dDDH values between strains C3-1-R+6T and

Parapedobacter lycopersici

KACC 18788T were 72, 75, and 18% respectively. Meanwhile, the ANI/AAI and dDDH values between these five isolates were higher than the threshold values, showing that they belonged to the same species of

Parapedobacter

. According to genome comparison, the novel isolates have some special biosynthetic gene clusters of secondary metabolites including bacteriton, aryl-polyene, lantipeptide and t1pks, which were absent from their most related phylogenetic neighbours

P. lycopersici

KACC 18788T and

P. pyrenivorans

CGMCC 1.12195T. The main polar lipids contained phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid, one unidentified glycolipid and five unidentified lipids. The predominant respiratory quinone was MK-7. The major cellular fatty acids were iso-C15 : 0, summed feature 3 (C16 : 1
 ω7c and/or C16 : 1
 ω6c) and iso-C17 : 0 3-OH. The genome size of strain C3-1-R+6T was 5 984 948 bp, and its genomic DNA G+C content was 46.21 mol%. To sum up, the five strains were identified as a novel species of the genus

Parapedobacter

, for which the name Parapedobacter tibetensis sp. nov. was proposed. The type strain was C3-1-R+6T (=CGMCC 1.19194T=KCTC 92150T).
A novel proteinaceous molecule produced by Lysinibacillus sp. OF-1 depends on the Ami oligopeptide transporter to kill Streptococcus pneumoniae
Authors: Hauge Ingvild Hals , Sandegren Vilde , Winther Anja Ruud , Bøe Cathrine Arnason , Salehian Zhian , Håvarstein Leiv Sigve , Kjos Morten , Straume Daniel
Published: March 2023

Infections caused by antibiotic-resistant

Streptococcus pneumoniae

are of growing concern for healthcare systems, which need new treatment options. Screening microorganisms in terrestrial environments has proved successful for discovering antibiotics, while production of antimicrobials by marine microorganisms remains underexplored. Here we have screened microorganisms sampled from the Oslo Fjord in Norway for production of molecules that prevent the human pathogen

S. pneumoniae

from growing. A bacterium belonging to the genus

Lysinibacillus

was identified. We show that this bacterium produces a molecule that kills a wide range of streptococcal species. Genome mining in BAGEL4 and AntiSmash suggested that it was a new antimicrobial compound, and we therefore named it lysinicin OF. The compound was resistant to heat (100 °C) and polymyxin acylase but susceptible to proteinase K, showing that it is of proteinaceous nature, but most probably not a lipopeptide.

S. pneumoniae

became resistant to lysinicin OF by obtaining suppressor mutations in the ami locus, which encodes the AmiACDEF oligo peptide transporter. We created ΔamiC and ΔamiEF mutants to show that pneumococci expressing a compromised Ami system were resistant to lysinicin OF. Furthermore, by creating mutants expressing an intact but inactive Ami system (AmiED184A and AmiFD175A) we could conclude that the lysinicin OF activity depended on the active form (ATP-hydrolysing) of the Ami system. Microscopic imaging and fluorescent labelling of DNA showed that

S. pneumoniae

treated with lysinicin OF had an average reduced cell size with condensed DNA nucleoid, while the integrity of the cell membrane remained intact. The characteristics and possible mode of action of lysinicin OF are discussed.
A model of health-related quality of life during COVID-19 pandemic: the role of food customer loyalty and affordability
Authors: Sumaedi Sik , Sumardjo Sumardjo , Saleh Amiruddin , Syukri Agus Fanar
Published: March 2023

Purpose

This research aims to test the simultaneous effects of the perceived threat of COVID-19, e-health literacy, e-health access barrier, loyalty toward healthy foods in general, loyalty toward functional foods, the affordability of healthy foods in general and the affordability of functional foods on health-related quality of life (HrQoL) during the COVID-19 pandemic.

Design/methodology/approach

A survey with 400 respondents in Banten, Indonesia, was performed. The data were analyzed using multiple regression analysis.

Findings

The results of the research showed that HrQoL during the COVID-19 pandemic was positively affected by e-health literacy and the affordability of healthy foods in general while negatively affected by the e-health access barrier, the perceived threat of COVID-19 and loyalty toward healthy foods in general. Furthermore, HrQoL was not influenced by loyalty toward and affordability of functional foods.

Research limitations/implications

This research was conducted in Banten. Due to the operational limitations during the COVID-19 pandemic, this research used a purposive sampling technique. Therefore, the next research should retest the model in different contexts and locations.

Practical implications

To improve HrQoL during the COVID-19 pandemic, citizens need to be educated on finding and utilizing credible online health information during the COVID-19 pandemic. Governments and health service providers should also strive to offer ease of access to credible online health information. Furthermore, the affordability of healthy foods, in general, should be managed well.

Originality/value

A few studies on HrQoL during COVID-19 pandemic were performed. However, there is a lack of paper that examines the role of food customer loyalty and affordability in a model of HrQoL during the COVID-19 pandemic. To the best of the authors’ knowledge, this paper is the first that involved and tested the role of food customer loyalty and affordability in a model of HrQoL during the COVID-19 pandemic.
Whale shark abundance forecast: The interannual hotspot effect
Authors: Jaramillo‐Gil Salomé , Pardo Mario A. , Vázquez‐Haikin Abraham , Bolaños‐Jiménez Jaime , Sosa‐Nishizaki Oscar
Published: March 2023

Each year, whale sharks (Rhincodon typus) aggregate in the northern Gulf of California at Bahia de los Angeles, a small coastal village that recently developed whale shark swimming as economic activity during the summer and autumn. Since the number of animals using the bay fluctuates annually, we aimed to predict it 1 month before each whale shark season. We based our modelling approach on the hypothesis that interannual warming conditions would spatially restrict whale shark habitats, triggering larger aggregations in areas that remain productive.

We photo‐identified whale sharks while surface feeding in Bahia de los Angeles during a 13‐year period, which allowed us to estimate a local abundance index per year through mark–recapture techniques. Then, we fitted these abundances as a function of several competing model structures, based on means of sea surface temperature and chlorophyll‐a, as well as their interannual anomalies from the linear trend, during spring, within the best‐known range of the species in the northern Gulf of California.

The results of the ecological model showed that whale sharks visit Bahia de los Angeles in larger numbers during extreme warming conditions, with relatively high surface chlorophyll‐a concentrations, which was especially evident during the 2014–2016 northeast Pacific Marine Heatwave and El Niño. Presumably, the overall reduction in surface productivity associated with these events forced whale sharks to restrict their summer–autumn habitat to productivity hotspots, including Bahia de los Angeles.

Our model allowed us to predict the number of animals visiting the site before each season starts, which has crucial proactive management implications, such as allowing authorities to regulate the ecotourism effort dynamically to reduce the probability of ship strikes and/or excessive stress to the animals. We propose an abundance baseline from the prediction for neutral interannual conditions.

Synthesis and applications. We recommend local authorities apply the model described in this study during the late spring of each year to obtain predictions of the upcoming whale shark abundance and establish the maximum ecotourism effort allowed accordingly.
MIGRANT URBANISM: Cape Verdean Djunta‐mon and its Impact on the Built Environment of Cova da Moura (Lisbon)
Authors: Cuberos‐Gallardo Francisco J.
Published: March 2023

International migrations are transforming the urban landscape in cities all around the world. In this article I address the effect of Cape Verdean migration on the built environment of Lisbon's periphery. Specifically, I analyse a traditional practice of mutual aid originating in rural Cape Verde, the djunta‐mon system, as a device of migrant urbanism projected onto the urban landscape of the Cova da Moura neighbourhood. In this article, which is based on ethnographic fieldwork, I discuss the role of the djunta‐mon in the urban evolution of the neighbourhood from its formation in the 1970s to the present day. The data collected shows that this practice has had an important effect on the appropriation, functional delimitation and significance of the urban space by residents. The results thus point to the importance of understanding how the cultures and identities of human groups crystallize through the processes of social production of space.
Replicate DNA metabarcoding can discriminate seasonal and spatial abundance shifts in river macroinvertebrate assemblages
Authors: Bush A. , Compson Z. , Rideout N. K. , Levenstein B. , Kattilakoski M. , Hajibabaei M. , Monk W. A. , Wright M. T. G. , Baird D. J.
Published: March 2023

The delivery of consistent and accurate fine‐resolution data on biodiversity using metabarcoding promises to improve environmental assessment and research. Whilst this approach is a substantial improvement upon traditional techniques, critics note that metabarcoding data are suitable for establishing taxon occurrence, but not abundance. We propose a novel hierarchical approach to recovering abundance information from metabarcoding, and demonstrate this technique using benthic macroinvertebrates. To sample a range of abundance structures without introducing additional changes in composition, we combined seasonal surveys with fish‐exclusion experiments at Catamaran Brook in northern New Brunswick, Canada. Five monthly surveys collected 31 benthic samples for DNA metabarcoding divided between caged and control treatments. A further six samples per survey were processed using traditional morphological identification for comparison. By estimating the probability of detecting a single individual, multispecies abundance models infer changes in abundance based on changes in detection frequency. Using replicate detections of 184 genera (and 318 species) from metabarcoding samples, our analysis identified changes in abundance arising from both seasonal dynamics and the exclusion of fish predators. Counts obtained from morphological samples were highly variable, a feature that limited the opportunity for more robust comparison, and emphasizing the difficulty standard methods also face to detect changes in abundance. Our approach is the first to demonstrate how quantitative estimates of abundance can be made using metabarcoding, both among species within sites as well as within species among sites. Many samples are required to capture true abundance patterns, particularly in streams where counts are highly variable, but few studies can afford to process entire samples. Our approach allows study of responses across whole communities, and at fine taxonomic resolution. We discuss how ecological studies can use additional sampling to capture changes in abundance at fine resolution, and how this can complement broad‐scale biomonitoring using DNA metabarcoding.
Evaluation of practices used to reduce the incidence of bovine respiratory disease in Australian feedlots (to November 2021)
Authors: Cusack PMV
Published: March 2023

Bovine respiratory disease (BRD) has been identified as the most significant infectious disease of feedlot cattle in eastern Australia.1Bovine respiratory disease causes economic loss due to medication costs, mortalities, excessive feed inputs associated with increased time on feed, reduced sale prices and associated labour costs. Bovine respiratory disease is a complex multifactorial condition with multiple animal, environmental and management risk factors predisposing cattle to illness. A range of microorganisms are implicated in BRD with at least four viral and five bacterial species commonly involved individually or in combination. The viruses most commonly associated with BRD in Australia are bovine herpesvirus 1 (BHV1), bovine viral diarrhoea virus (BVDV or bovine pestivirus), bovine parainfluenza 3 virus (PI3) and bovine respiratory syncytial virus (BRSV). More recently, bovine coronavirus has been identified as a potential viral contributor to BRD in Australia.2A number of bacterial species have also been recognised as important to the BRD complex; these includeMannheimia haemolytica,Pasteurella multocida,Histophilus somni,Trueperella pyogenesandMycoplasma bovis. Although one or more of the pathogens listed above can be isolated from clinical cases of BRD, there is no evidence that infection alone causes serious illness. This indicates that, in addition to specific infectious agents, other factors are crucial for the development of BRD under field conditions. These can be categorised as environmental, animal and management risk factors. These risk factors are likely to exert their effects through multiple pathways including reductions in systemic and possibly local immunity. For example, stressors such as weaning, handling at saleyards, transport, dehydration, weather conditions, dietary changes, comingling and pen competition might reduce the effectiveness of the immune system. Reduced immunocompetence can allow opportunistic infection of the lower airways with potential pathogens leading to the development of BRD. The objective of this paper is to critically review the evidence for management practices aimed at reducing the incidence of BRD in Australian feedlot cattle. Predisposing factors (Table 1) largely beyond the control of most feedlots, such as weather and exposure to respiratory viruses, are discussed separately, but these factors can generate indirect prevention responses that are discussed under the preventative practices categories. The current practices are classified as either animal preparation practices (Table 2) or feedlot management practices (Table 3).
Psychological distress and workplace risk inequalities among conservation professionals
Authors: Pienkowski Thomas , Keane Aidan , de Lange Emiel , Kapoor Vena , Khanyari Munib , Ravi Roshni , Smit Izak P. J. , Castelló y Tickell Sofia , Hazenbosch Mirjam , Arlidge William N. S. , Baranyi Gergő , Brittain Stephanie , Papworth Sarah , Saxena Sonakshi , Hout Vimean , Milner‐Gulland E. J.
Published: March 2023

Workplaces can be sources of both stress and support, affecting employees' mental health and productivity. Yet, little research has investigated variability in workplace risk factors for poor mental health in conservation. We aimed to explore how patterns of psychological distress—a state of emotional disturbance—and associated workplace risk factors vary between conservation job roles. Working with three case study organizations in India, South Africa, and Cambodia, we surveyed 280 field‐based, office‐based, and research staff. Moderate or severe psychological distress was reported by 28.9%. Field‐based practitioners reported a greater imbalance between workplace efforts and rewards (0.35 standard deviation (SD), 95% credibility interval (CI) 0.03–0.67) than their colleagues, which was associated with greater psychological distress (0.24 SD, 95% CI 0.10–0.39). After controlling for this mediated relationship, researchers reported greater psychological distress than field‐based practitioners (0.37 SD, 95% CI 0.02–0.72). However, when accounting for all direct and indirect effects, there was no overall difference in distress between roles. Employers, funders, professional societies, and other institutions seeking to support conservationists' mental health should understand and offer support tailored to role‐specific challenges. Doing so might enhance conservationists' wellbeing while strengthening their ability to reverse global nature loss.
Thymectomy in thymomatous generalized myasthenia gravis: An analysis of the prognosis and risk factors
Authors: Zhang Jinwei , Zhang Peng , Zhang Hui , Cui Yuantao , Chen Yuan , Lv Peng , Li Xin , Zhang Peng
Published: March 2023

Background and purpose

This study investigated the postthymectomy outcomes and factors affecting the prognosis of thymomatous generalized myasthenia gravis (TGMG).

Methods

Clinical records of 86 patients with TGMG who underwent thymectomy at our institution between 2012 and 2020 were retrospectively reviewed. Predictors of complete stable remission (CSR) and exacerbation were analyzed using multivariate regression analysis.

Results

A total of 16 patients achieved CSR, four achieved pharmacological remission, six exhibited deterioration, and eight died of myasthenia gravis (MG; mean follow‐up = 75.1 months). Male sex (p = 0.049) and disease duration < 11.5 weeks before surgery (p = 0.003) were significant positive predictors of CSR. Onset age < 52.8 years and symptoms of ocular and limb muscle weakness had a higher CSR rate than onset age > 52.8 years (p = 0.056) and symptoms of bulbar muscles (p = 0.071). Female patients had a significant higher risk of exacerbation (p = 0.042).

Conclusions

Male sex and disease duration < 11.5 weeks were independent predictors of CSR in TGMG postthymectomy. Onset age < 52.8 years and ocular and limb muscle weakness at onset were associated with a higher probability of achieving CSR than onset age > 52.8 years and bulbar muscle weakness. Female sex was an independent predictor of MG symptom exacerbation in TGMG postthymectomy.
Relation and effect of resilience on burnout in nurses: A literature review and meta‐analysis
Authors: Castillo‐González Andrea , Velando‐Soriano Almudena , De La Fuente‐Solana Emilia I. , Martos‐Cabrera Begoña M. , Membrive‐Jiménez María José , Lucía Ramírez‐Baena , Cañadas‐De La Fuente Guillermo A.
Published: March 2023

Aim

To study the relation between burnout and resilience and to identify the profile of nurses presenting this quality.

Background and Introduction

Healthcare professionals are subject to high rates of burnout. Resilience could be an important factor in preventing or alleviating this condition.

Methods

The PubMed, ProQuest, Scopus and ScienceDirect databases were consulted in February 2022 using the equation ‘burnout AND resilience AND nurs*’. The inclusion criteria applied were that the texts should describe quantitative studies, be published in English or Spanish, in any year, and be directly related to the question considered. The meta‐analysis was performed using StatsDirect statistical software.

Results

Analysis of the 29 studies shows that among the dimensions of burnout, nurses are especially prone to emotional exhaustion, and are less affected by depersonalisation and low personal accomplishment. Those who score highly for resilience tend to have longer service experience, acceptable salaries and less work overload. Meta‐analysis reveals an inverse correlation between resilience and burnout (r= −0.41;n= 2750), exhaustion (r= −0.27;n= 6966) and depersonalisation (r= −0.23;n= 6115).

Conclusion

Many nurses present low levels of resilience and suffer from burnout syndrome. The application of programmes to enhance their resilience would help prevent burnout and optimise the potential to provide quality health care.

Implications for nursing and health policy

Resilience is inversely correlated with burnout, depersonalisation and emotional exhaustion. Accordingly, healthcare organisations should develop and implement evidence‐based programmes to foster nurses’ resilience and thus reduce their susceptibility to burnout.
Reassessment of the effect of space charge potential on the electrical conductivity of polycrystalline ceramics
Authors: Fang Tsang‐Tse , Lu Yu‐Chi , Hsiang Hsing‐I
Published: March 2023

The calculation of space charge potential, the difference of the activation energy of grain boundary and grain, and the ratio of conductivity of grain boundary and grain at different temperatures of the polycrystalline ceramics have been reappraised in the present model, in which charge carriers hopping through bulk and grain boundary barriers with different mobility are taken into account. It is evidenced that the present model is valid and more precise than previous models, and thus, it is concluded that the assumption that the mobility of charge carriers hopping through bulk and grain boundary barrier is the same made by the previous studies is unsuitable.
Application of subcutaneous radiofrequency after liposuction on midface: A minimally invasive technique for midface rejuvenation
Authors: Zou Chong , Wang Jiaqi , Wang Tailing
Published: March 2023

Background

Popular demand for minimally invasive soft tissue tightening is continuously on the rise. In recent years, subcutaneous radiofrequency or radiofrequency‐assisted liposuction (RFAL) has been reported to produce the desired surgical outcome on lower‐face and body tightening. However, few studies are found using subcutaneous radiofrequency for midface rejuvenation.

Aims

This study was performed to review our surgical method for the midface rejuvenation using subcutaneous radiofrequency combined with liposuction and to evaluate the clinical outcomes.

Patients/Methods

This retrospective observational study involved 31 patients with mild‐to‐moderate mid‐face laxity. All patients received liposuction combined with subcutaneous radiofrequency on midface from June 2020 to June 2022. Clinical results were assessed objectively using photographs and subjectively by a patient satisfaction survey.

Results

All patients recovered well without major complications. High patient satisfaction was attained. The mean score of midface laxity (GGS) as evaluated by jury decreased from 3.3 preoperatively to 1.6 postoperatively.

Conclusions

Our midface tightening technique is safe and effective for patients with a mild‐to‐moderate midface aging appearance.

Level of Evidence

Therapeutic IV.
Closure of Labor & Delivery units in rural counties is associated with reduced adequacy of prenatal care, even when prenatal care remains available
Authors: Radke Stephanie M. , Smeins Laurel , Ryckman Kelli K. , Gruca Thomas S.
Published: March 2023

Purpose

Closure of rural Labor & Delivery (L&D) units can impact timely access to hospital‐based obstetrical care. Iowa has lost over a quarter of its L&D units in the previous decade. Assessing the effect of these closures on prenatal care in those rural communities is important to understanding the full effect of unit closures on maternal health care.

Methods

Using birth certificate data in Iowa from 2017 to 2019, the initiation of prenatal care and adequacy of prenatal visits were assessed for 47 rural counties in Iowa. Of these, 7 experienced a closure of the only L&D unit between 1/1/2018 and 1/1/2019. The impact of these closures is modeled for all birthing parents and compared for Medicaid versus non‐Medicaid recipients.

Findings

All 7 counties that experienced the loss of their only L&D unit continued to have prenatal care services available. Experiencing a closure of an L&D unit was associated with a lower likelihood of overall adequate prenatal care but not significantly associated with a lower rate of first‐trimester prenatal care utilization. Among Medicaid recipients of the communities where an L&D unit closed, there was an association of closure with both a lower likelihood of adequate prenatal care and entry to prenatal care after the first trimester.

Conclusions

Utilization of prenatal care is lower in rural communities following L&D unit closure, especially among Medicaid recipients. This suggests that the overall maternal health systems were disrupted by the closure of the L&D unit, impacting the utilization of services that remained available to the community.
Morphological shifts in response to spatial sorting of dispersal behaviour in red flour beetles across multiple generations
Authors: Arnold P. A. , Cassey P. , White C. R.
Published: March 2023

Dispersal is a key component of life history that influences individual fitness, population dynamics and range expansion. Morphological traits that are functionally related to dispersal may change through the spatial sorting of phenotypes at population range edges and invasion fronts. Generally, since larger invertebrates are expected to have greater dispersal capacity, larger body size and mass is expected to evolve in dispersers. Yet, different study systems exhibit a range of trait‐specific and sex‐specific responses to spatial sorting. Furthermore, a persistent issue in this field is that spatial sorting can be challenging to separate from natural selection and population dynamics. To address this, here we investigated the responses of dispersal and morphological traits to simulated spatial sorting into disperser and non‐disperser red flour beetles (Tribolium castaneum) using a controlled laboratory dispersal system that isolated spatial sorting from natural selection and allowed sex‐specific dispersal traits to evolve independently. After seven generations of spatial sorting, the time taken to disperse decreased in dispersers and increased in non‐dispersers, with males dispersing more quickly than females. In contrast to expectations that dispersers would increase in body mass and size, we found the opposite. Body mass and morphology diverged such that dispersers became smaller and narrower, while non‐dispersers became larger relative to the randomly selected controls, but femur length did not change. The trait responses across generations were also sex specific. Divergence between male dispersers and non‐dispersers was more substantial than females, both in dispersal and in some morphological traits. We hypothesize that small individuals were more biomechanically efficient at dispersing through the dispersal apparatus and were therefore the ones that dispersed more readily. We suggest that key differences in dispersal modes across biological systems may impact the morphological and phenotypic trait changes from spatial sorting processes in range expansions and biological invasions.
The Structural Transformation of Thailand: The Role of Policy Distortion
Authors: TEMSUMRIT NAVARAT , SRIKET HONGSILP
Published: March 2023

Thailand and the Republic of Korea were developing at a similar pace during the 1960s. However, the Republic of Korea’s economic development rapidly expanded from the 1970s onward, leaving Thailand lagging far behind. This paper investigates the labor productivity slowdown in Thailand using a nine-sector, structural transformation model setting with policy distortions. Our findings suggest that the economic underdevelopment of Thailand, when compared with the Republic of Korea, lies in a tendency toward relative labor productivity slowdowns resulting from both direct and indirect policy distortions in the agriculture sector. The agriculture sector has consistently been overlooked by the government in favor of the infant industrial sector for many decades. Moreover, as the agriculture sector accounts for a relatively considerable share of employment, the magnitude of the negative impact from policy distortions is amplified, inducing a delay in Thailand’s structural transformation.
Chemical reactive second-grade nanofluid flow past an exponential curved stretching surface: Numerically
Authors: Abbas Nadeem , Ali Mohsin , Shatanawi Wasfi
Published: March 2023

The exponentially stretching curved surface is considered for second-grade fluid flow. The slip effects are applied at the assumed surface. The Brownian motion, chemical reaction, and thermophoresis effects are studied to determine the influence of the second-grade fluid. The boundary layer approximation is applied to the governing equation which is reduced in terms of partial differential equations. These partial differential equations become the dimensionless form after using the appropriate transformations. Further, the numerical approach is imposed on the dimensionless system to calculate the problem-related results. Graphs and tables are used to show how involving physical parameters have an effect. The velocity profile and skin friction have been increased due to increasing values of curvature parameters. The velocity function declined due to an increment in the second-grade fluid parameter. It means that the thickness of the boundary layer shown as decline when the second-grade fluid parameter rises while the second-grade fluid parameter has an inverse relation with viscosity. The Prandtl number increased which increased the temperature function. Physically, the thickness of the thermal boundary is controlled by the Prandtl number, and temperature increases by increasing the value of the Prandtl number.
EXTERNAL FIXATION COMBINED WITH A MODIFIED MINIMALLY INVASIVE AC JOINT RECONSTRUCTION SYSTEM IN ACUTE AND CHRONIC ACROMIOCLAVICULAR DISLOCATIONS
Authors: Nicola Lollino , Giulia Nicolaci , Alice Piccato , Marco Assom
Published: March 2023

Background: Acromioclavicular joint (ACJ) dislocations are among one of the most common injuries of the shoulder and still a challenge for surgeons. We propose a technique that combines the advantages of external fixation (to obtain a stable reduction of ACJ) and a modified suspension device system (for minimally invasive ACJ reconstruction) in acute dislocations. We tend to add associate augmentation of autologous semitendinosus graft to supply a biological reconstruction of the coracoclavicular (CC) ligaments in chronic dislocations.

Material and Methods: We enrolled 8 patients, 6 with acute and 2 with chronic injuries. Inclusion criteria were the following: types IV, V, and VI ACJ dislocations; type III ACJ dislocations in high-demand patients. Exclusion criteria were the following: asymptomatic chronic ACJ dislocation without functional impairment, previous coracoid or clavicle fracture, nonparticipating patients, alcohol abusers, previous infection, and neurological problems. The external fixator was removed at 40 days after surgery and patients were followed up clinically (through Constant Score and Simple Shoulder Test Questionnaires) and radiologically for the first three months, at six months and one year after surgery.

Results: In all cases, radiographic ACJ reduction was maintained at a minimum one-year follow-up. Steel wire breakage occurred in one patient but the retained tip in the clavicle was asymptomatic; we report no further complications. Shoulder active and passive range of motion was painless in all patients; the Constant score (CS) was 85/100 and the Simple Shoulder Test (SST) score was 9/10 on average at one year of follow-up.

Conclusions: Our technique demonstrated stable joint reduction and good functional outcomes at one-year follow-up and it seems to be promising, but more clinical and biomechanical studies are required to confirm these encouraging results.
Supplementary Table 6 from MicroRNA Signatures in Human Ovarian Cancer
Authors: Iorio Marilena V. , Visone Rosa , Di Leva Gianpiero , Donati Valentina , Petrocca Fabio , Casalini Patrizia , Taccioli Cristian , Volinia Stefano , Liu Chang-Gong , Alder Hansjuerg , Calin George A. , Ménard Sylvie , Croce Carlo M.
Published: March 2023

Supplementary Table 6 from MicroRNA Signatures in Human Ovarian Cancer
Supplementary Figure 2 from MicroRNA Signatures in Human Ovarian Cancer
Authors: Iorio Marilena V. , Visone Rosa , Di Leva Gianpiero , Donati Valentina , Petrocca Fabio , Casalini Patrizia , Taccioli Cristian , Volinia Stefano , Liu Chang-Gong , Alder Hansjuerg , Calin George A. , Ménard Sylvie , Croce Carlo M.
Published: March 2023

Supplementary Figure 2 from MicroRNA Signatures in Human Ovarian Cancer
Supplementary Figure 1 from MicroRNA Signatures in Human Ovarian Cancer
Authors: Iorio Marilena V. , Visone Rosa , Di Leva Gianpiero , Donati Valentina , Petrocca Fabio , Casalini Patrizia , Taccioli Cristian , Volinia Stefano , Liu Chang-Gong , Alder Hansjuerg , Calin George A. , Ménard Sylvie , Croce Carlo M.
Published: March 2023

Supplementary Figure 1 from MicroRNA Signatures in Human Ovarian Cancer
Supplementary Data from Glutathione S-Transferase P1: Gene Sequence Variation and Functional Genomic Studies
Authors: Moyer Ann M. , Salavaggione Oreste E. , Wu Tse-Yu , Moon Irene , Eckloff Bruce W. , Hildebrandt Michelle A.T. , Schaid Daniel J. , Wieben Eric D. , Weinshilboum Richard M.
Published: March 2023

Supplementary Data from Glutathione S-Transferase P1: Gene Sequence Variation and Functional Genomic Studies
Supplementary Table 1 from Genome-Scale MicroRNA and Small Interfering RNA Screens Identify Small RNA Modulators of TRAIL-Induced Apoptosis Pathway
Authors: Ovcharenko Dmitriy , Kelnar Kevin , Johnson Charles , Leng Nan , Brown David
Published: March 2023

Supplementary Table 1 from Genome-Scale MicroRNA and Small Interfering RNA Screens Identify Small RNA Modulators of TRAIL-Induced Apoptosis Pathway
Supplementary Table 1 from Mitochondrial/Cell-Surface Protein p32/gC1qR as a Molecular Target in Tumor Cells and Tumor Stroma
Authors: Fogal Valentina , Zhang Lianglin , Krajewski Stan , Ruoslahti Erkki
Published: March 2023

Supplementary Table 1 from Mitochondrial/Cell-Surface Protein p32/gC1qR as a Molecular Target in Tumor Cells and Tumor Stroma
Supplementary Tables 1-4 from A Transgenic Mouse Model of Plasma Cell Malignancy Shows Phenotypic, Cytogenetic, and Gene Expression Heterogeneity Similar to Human Multiple Myeloma
Authors: Boylan Kristin L.M. , Gosse Mary A. , Staggs Sarah E. , Janz Siegfried , Grindle Suzanne , Kansas Geoffrey S. , Van Ness Brian G.
Published: March 2023

Supplementary Tables 1-4 from A Transgenic Mouse Model of Plasma Cell Malignancy Shows Phenotypic, Cytogenetic, and Gene Expression Heterogeneity Similar to Human Multiple Myeloma
Supplementary Figures 1-7 from Hoxb7 Inhibits Transgenic HER-2/neu–Induced Mouse Mammary Tumor Onset but Promotes Progression and Lung Metastasis
Authors: Chen Hexin , Lee Ji Shin , Liang Xiaohui , Zhang Huiping , Zhu Tao , Zhang Zhe , Taylor M. Evangeline , Zahnow Cynthia , Feigenbaum Lionel , Rein Alan , Sukumar Saraswati
Published: March 2023

Supplementary Figures 1-7 from Hoxb7 Inhibits Transgenic HER-2/neu–Induced Mouse Mammary Tumor Onset but Promotes Progression and Lung Metastasis
Supplementary Figure 4 from Reactive Oxygen Species Regulate Angiogenesis and Tumor Growth through Vascular Endothelial Growth Factor
Authors: Xia Chang , Meng Qiao , Liu Ling-Zhi , Rojanasakul Yongyut , Wang Xin-Ru , Jiang Bing-Hua
Published: March 2023

Supplementary Figure 4 from Reactive Oxygen Species Regulate Angiogenesis and Tumor Growth through Vascular Endothelial Growth Factor
Supplementary Table 4 from Genome-Wide Analysis of Aromatase Inhibitor-Resistant, Tamoxifen-Resistant, and Long-Term Estrogen-Deprived Cells Reveals a Role for Estrogen Receptor
Authors: Masri Selma , Phung Sheryl , Wang Xin , Wu Xiwei , Yuan Yate-Ching , Wagman Lawrence , Chen Shiuan
Published: March 2023

Supplementary Table 4 from Genome-Wide Analysis of Aromatase Inhibitor-Resistant, Tamoxifen-Resistant, and Long-Term Estrogen-Deprived Cells Reveals a Role for Estrogen Receptor
Supplementary Figures 1-5, Table 1 from Identification of Pbx1, a Potential Oncogene, as a Notch3 Target Gene in Ovarian Cancer
Authors: Park Joon T. , Shih Ie-Ming , Wang Tian-Li
Published: March 2023

Supplementary Figures 1-5, Table 1 from Identification of Pbx1, a Potential Oncogene, as a Notch3 Target Gene in Ovarian Cancer
Supplementary Tables S1 and S2 from Silencing of DUOX NADPH Oxidases by Promoter Hypermethylation in Lung Cancer
Authors: Luxen Sylvia , Belinsky Steven A. , Knaus Ulla G.
Published: March 2023

Supplementary Tables S1 and S2 from Silencing of DUOX NADPH Oxidases by Promoter Hypermethylation in Lung Cancer
Supplementary Figure Legend, Methods and Materials from Retinoblastoma Function Is Essential for Establishing Lung Epithelial Quiescence after Injury
Authors: Mason-Richie Nicole A. , Mistry Meenakshi J. , Gettler Caitlin A. , Elayyadi Asmaa , Wikenheiser-Brokamp Kathryn A.
Published: March 2023

Supplementary Figure Legend, Methods and Materials from Retinoblastoma Function Is Essential for Establishing Lung Epithelial Quiescence after Injury
Supplementary Figures 1-3 from Knockdown of the Transforming Growth Factor-β Type III Receptor Impairs Motility and Invasion of Metastatic Cancer Cells
Authors: Criswell Tracy L. , Dumont Nancy , Barnett Joey V. , Arteaga Carlos L.
Published: March 2023

Supplementary Figures 1-3 from Knockdown of the Transforming Growth Factor-β Type III Receptor Impairs Motility and Invasion of Metastatic Cancer Cells
Supplementary Figure 3 from Development of Cellular Immune Responses against PAX5, a Novel Target for Cancer Immunotherapy
Authors: Yan Mengyong , Himoudi Nourredine , Pule Martin , Sebire Neil , Poon Edmund , Blair Allison , Williams Owen , Anderson John
Published: March 2023

Supplementary Figure 3 from Development of Cellular Immune Responses against PAX5, a Novel Target for Cancer Immunotherapy
Supplementary Materials and Methods from Oct-3/4 Expression Reflects Tumor Progression and Regulates Motility of Bladder Cancer Cells
Authors: Chang Chao-Ching , Shieh Gia-Shing , Wu Pensee , Lin Chia-Cheng , Shiau Ai-Li , Wu Chao-Liang
Published: March 2023

Supplementary Materials and Methods from Oct-3/4 Expression Reflects Tumor Progression and Regulates Motility of Bladder Cancer Cells
Supplementary Table 1 from Attenuation of Melanoma Invasion by a Secreted Variant of Activated Leukocyte Cell Adhesion Molecule
Authors: van Kilsdonk Jeroen W.J. , Wilting Roel H. , Bergers Mieke , van Muijen Goos N.P. , Schalkwijk Joost , van Kempen Léon C.L.T. , Swart Guido W.M.
Published: March 2023

Supplementary Table 1 from Attenuation of Melanoma Invasion by a Secreted Variant of Activated Leukocyte Cell Adhesion Molecule
Supplementary Figure 2 from Attenuation of Melanoma Invasion by a Secreted Variant of Activated Leukocyte Cell Adhesion Molecule
Authors: van Kilsdonk Jeroen W.J. , Wilting Roel H. , Bergers Mieke , van Muijen Goos N.P. , Schalkwijk Joost , van Kempen Léon C.L.T. , Swart Guido W.M.
Published: March 2023

Supplementary Figure 2 from Attenuation of Melanoma Invasion by a Secreted Variant of Activated Leukocyte Cell Adhesion Molecule
Supplementary Figure 4 from Fibroblasts Isolated from Common Sites of Breast Cancer Metastasis Enhance Cancer Cell Growth Rates and Invasiveness in an Interleukin-6–Dependent Manner
Authors: Studebaker Adam W. , Storci Gianluca , Werbeck Jillian L. , Sansone Pasquale , Sasser A. Kate , Tavolari Simona , Huang Tim , Chan Michael W.Y. , Marini Frank C. , Rosol Thomas J. , Bonafé Massimiliano , Hall Brett M.
Published: March 2023

Supplementary Figure 4 from Fibroblasts Isolated from Common Sites of Breast Cancer Metastasis Enhance Cancer Cell Growth Rates and Invasiveness in an Interleukin-6–Dependent Manner
Supplementary Figure Legend from Nicotine Promotes Mammary Tumor Migration via a Signaling Cascade Involving Protein Kinase C and cdc42
Authors: Guo Jinjin , Ibaragi Soichiro , Zhu Tongbo , Luo Ling-Yu , Hu Guo-Fu , Huppi Petra S. , Chen Chang Yan
Published: March 2023

Supplementary Figure Legend from Nicotine Promotes Mammary Tumor Migration via a Signaling Cascade Involving Protein Kinase C and cdc42
Supplementary Figure 3 from Fibroblasts Isolated from Common Sites of Breast Cancer Metastasis Enhance Cancer Cell Growth Rates and Invasiveness in an Interleukin-6–Dependent Manner
Authors: Studebaker Adam W. , Storci Gianluca , Werbeck Jillian L. , Sansone Pasquale , Sasser A. Kate , Tavolari Simona , Huang Tim , Chan Michael W.Y. , Marini Frank C. , Rosol Thomas J. , Bonafé Massimiliano , Hall Brett M.
Published: March 2023

Supplementary Figure 3 from Fibroblasts Isolated from Common Sites of Breast Cancer Metastasis Enhance Cancer Cell Growth Rates and Invasiveness in an Interleukin-6–Dependent Manner
Supplementary Figure Legends 1-3 from Dissecting and Targeting the Growth Factor–Dependent and Growth Factor–Independent Extracellular Signal-Regulated Kinase Pathway in Human Schwannoma
Authors: Ammoun Sylwia , Flaiz Christine , Ristic Natalia , Schuldt Jennifer , Hanemann C. Oliver
Published: March 2023

Supplementary Figure Legends 1-3 from Dissecting and Targeting the Growth Factor–Dependent and Growth Factor–Independent Extracellular Signal-Regulated Kinase Pathway in Human Schwannoma
Supplementary Figures 1-6 from CSN5 Isopeptidase Activity Links COP9 Signalosome Activation to Breast Cancer Progression
Authors: Adler Adam S. , Littlepage Laurie E. , Lin Meihong , Kawahara Tiara L.A. , Wong David J. , Werb Zena , Chang Howard Y.
Published: March 2023

Supplementary Figures 1-6 from CSN5 Isopeptidase Activity Links COP9 Signalosome Activation to Breast Cancer Progression
Supplementary Video 8 from Vimentin Filaments Support Extension of Tubulin-Based Microtentacles in Detached Breast Tumor Cells
Authors: Whipple Rebecca A. , Balzer Eric M. , Cho Edward H. , Matrone Michael A. , Yoon Jennifer R. , Martin Stuart S.
Published: March 2023

Supplementary Video 8 from Vimentin Filaments Support Extension of Tubulin-Based Microtentacles in Detached Breast Tumor Cells
Supplementary Figure 2 from DNA Damage–Dependent Translocation of B23 and p19ARF Is Regulated by the Jun N-Terminal Kinase Pathway
Authors: Yogev Orli , Saadon Keren , Anzi Shira , Inoue Kazushi , Shaulian Eitan
Published: March 2023

Supplementary Figure 2 from DNA Damage–Dependent Translocation of B23 and p19ARF Is Regulated by the Jun N-Terminal Kinase Pathway