bluuebunny/crossref_metadata_2025 huggingface dataset:
Natural variation in the plant polyadenylation complex
Authors: Zhou Lichun , Li Kai , Hunt Arthur G.
Published: January 2024

Messenger RNA polyadenylation, the process wherein the primary RNA polymerase II transcript is cleaved and a poly(A) tract added, is a key step in the expression of genes in plants. Moreover, it is a point at which gene expression may be regulated by determining the functionality of the mature mRNA. Polyadenylation is mediated by a complex (the polyadenylation complex, or PAC) that consists of between 15 and 20 subunits. While the general functioning of these subunits may be inferred by extending paradigms established in well-developed eukaryotic models, much remains to be learned about the roles of individual subunits in the regulation of polyadenylation in plants. To gain further insight into this, we conducted a survey of variability in the plant PAC. For this, we drew upon a database of naturally-occurring variation in numerous geographic isolates of Arabidopsis thaliana. For a subset of genes encoding PAC subunits, the patterns of variability included the occurrence of premature stop codons in some Arabidopsis accessions. These and other observations lead us to conclude that some genes purported to encode PAC subunits in Arabidopsis are actually pseudogenes, and that others may encode proteins with dispensable functions in the plant. Many subunits of the PAC showed patterns of variability that were consistent with their roles as essential proteins in the cell. Several other PAC subunits exhibit patterns of variability consistent with selection for new or altered function. We propose that these latter subunits participate in regulatory interactions important for differential usage of poly(A) sites.
Legacy of culture heritage building revitalization: place attachment and culture identity
Authors: Chan Suk Ha Grace , Lee Wing Han Helen , Tang Binglin Martin , Chen Ziyi
Published: January 2024

Introduction

Cultural heritage buildings are revitalized to promote culture instead of being neglected or demolished. For locals, the revitalization of heritage buildings symbolizes a commitment to the community and a taste of collective memory. The study attempts to test the effectiveness of heritage cultural building on visitors’ post-experience behavior through cultural identity and place attachment. “Cultural activities engagement” and “knowledge transfer” serves as moderating attributes. Their moderating the effects on cultural identity and place attachment are examined, respectively.

Method

A valid sample size of 348 from four heritage buildings located in Hong Kong: Tai Kwun, Police Married Quarters (PMQ), Mei Ho House and The Mills. A data analysis platform for PLSSEM is chosen for this study.

Results

Results demonstrate that the effectiveness of heritage building revitalization on visitors’ local cultural identity and emotions leading to place attachment.

Discussion

In our study, the effectiveness of heritage building encompasses three elements which includes appearance and components; technology and planning as well as contribution. Knowledge transfer positively moderates the effectiveness of heritage building revitalization and cultural identity. However, cultural activity engagement negatively moderates place attachment. Findings also exhibit that place attachment in heritage building revitalization leads to a positive experience extension. For the practical contribution, the study provides insights to policy makers and planners for historic building design such as appearance and components, technology in facilitating local visitors’ local identity.
Learning analytics for enhanced professional capital development: a systematic review
Authors: de La Hoz-Ruiz Javier , Khalil Mohammad , Domingo Segovia Jesús , Liu Qinyi
Published: January 2024

Background/Motivation

This article presents a systematic review aimed at examining the utilization of learning analytics (LA) to enhance teachers’ professional capital.

Aim

The study focuses on three primary research questions: (1) exploring the characteristics and approaches of LA in professional capital, (2) investigating suggestions from LA for assessing and improving professional capital, and (3) examining variables studied in enhancing the most intricate dimension of professional capital using LA.

Methodology

To address the research objectives, a systematic review was conducted focusing on the key concepts “learning analytics” and “professional capital.” Following the procedures outlined encompassed in four stages: identification, screening, inclusion, and adequacy. The PRISMA 2009 protocol guided the systematic review process.

Principal findings

The findings of the study underscore the efficacy of LA as a catalyst for improving professional capital, particularly through collaborative learning and the utilization of tools like forums and online learning platforms. Social capital emerges as a pivotal component in integrating diverse types of professional capital, fostering opportunities for knowledge creation and social networking.

Conclusion/Significance

In conclusion, the study highlights the paramount significance of addressing teachers’ professional capital development through collaborative approaches and leveraging technology, particularly in primary education. The article concludes by emphasizing the imperative for more research and knowledge dissemination in this field, aiming to ensure equity in learning and address the challenges posed by the COVID−19 pandemic.
Current trends in psychotherapies and psychosocial interventions for people with dementia: a scoping review of randomized controlled trials
Authors: Vicente Célia , Fernandes Sónia , Romão Ana , Fernandes Júlio Belo
Published: January 2024

An outcome of dementia is a progressive decline in cognitive function. Implementing psychotherapies and psychosocial interventions is crucial for bolstering cognitive abilities, promoting independence, and elevating the quality of life for individuals with dementia. This review aims to identify current trends in psychotherapies and psychosocial interventions for people with dementia. A Scoping review was developed based on the framework proposed by Arksey and O’Malley. The literature search was conducted on electronic databases, including Scopus, Cochrane Central Register of Controlled Trials, MEDLINE, CINAHL, Nursing & Allied Health Collection, and MedicLatina. Executed in June 2023, the search focused on articles published in English, Portuguese, and Spanish between 2013 and 2023. Through this search, 1409 articles were initially identified. After selecting and analyzing the reports, sixteen trials were included in this review. Eight distinct categories were identified, covering different strategies. These categories run from computerized game-based cognitive training and reminiscence therapy to compensatory and restorative strategies, memory and attention training, calculation training, dual-task training, counseling, and personalized goal attainment. The findings of this scoping review highlight the diverse landscape of psychotherapies and psychosocial interventions for people with dementia.
Understanding profound autism: implications for stigma and supports
Authors: Clarke Elaine B. , McCauley James B. , Lutz Amy , Gotelli Marina , Sheinkopf Stephen J. , Lord Catherine
Published: January 2024

Introduction

Societal perceptions and lack of understanding of autism spectrum disorder can be stigmatizing for autistic individuals and their families. This may be particularly the case for individuals who meet criteria for profound autism. Despite the considerable service needs of this marginalized group, there is little data on the prevalence of profound autism, nor on the experiences of those with profound autism and their families.

Methods

The current study leveraged a mixed-methods approach to address these gaps. First, the prevalence of profound autism was examined in six samples—three from the United States and three from Western Europe. Second, inductive thematic analysis was used to code interviews from 20 caregivers of profoundly autistic adults.

Results

The prevalence of profound autism varied widely across the six samples—from 11% to 48%. There were also notable differences between samples in prevalence by gender, race, and ethnicity. Two overarching themes were identified via inductive thematic analysis: Community Perceptions of Autism and Family Support Needs and Advocacy Challenges. Though caregivers were not directly asked about stigmatization during interviews, 85% of caregivers reported at least one instance of perceived stigma.

Discussion

Future research should continue to examine the unique needs and stigmatization experiences of profoundly autistic individuals and their families across the life course.
Prevalence, predictors, and patient-reported outcomes of long COVID in hospitalized and non-hospitalized patients from the city of São Paulo, Brazil
Authors: Malheiro Daniel Tavares , Bernardez-Pereira Sabrina , Parreira Kauê Capellato Junqueira , Pagliuso João Gabriel Dias , de Paula Gomes Emerson , de Mesquita Escobosa Daisa , de Araújo Carolina Ivo , Pimenta Beatriz Silva , Lin Vivian , de Almeida Silvana Maria , Tuma Paula , Laselva Claudia Regina , Neto Miguel Cendoroglo , Klajner Sidney , Teich Vanessa Damazio , Kobayashi Takaaki , Edmond Michael B. , Marra Alexandre R.
Published: January 2024

Background

Robust data comparing long COVID in hospitalized and non-hospitalized patients in middle-income countries are limited.

Methods

A retrospective cohort study was conducted in Brazil, including hospitalized and non-hospitalized patients. Long COVID was diagnosed at 90-day follow-up using WHO criteria. Demographic and clinical information, including the depression screening scale (PHQ-2) at day 30, was compared between the groups. If the PHQ-2 score is 3 or greater, major depressive disorder is likely. Logistic regression analysis identified predictors and protective factors for long COVID.

Results

A total of 291 hospitalized and 1,118 non-hospitalized patients with COVID-19 were included. The prevalence of long COVID was 47.1% and 49.5%, respectively. Multivariable logistic regression showed female sex (odds ratio [OR] = 4.50, 95% confidence interval (CI) 2.51–8.37), hypertension (OR = 2.90, 95% CI 1.52–5.69), PHQ-2 > 3 (OR = 6.50, 95% CI 1.68–33.4) and corticosteroid use during hospital stay (OR = 2.43, 95% CI 1.20–5.04) as predictors of long COVID in hospitalized patients, while female sex (OR = 2.52, 95% CI 1.95–3.27) and PHQ-2 > 3 (OR = 3.88, 95% CI 2.52–6.16) were predictors in non-hospitalized patients.

Conclusion

Long COVID was prevalent in both groups. Positive depression screening at day 30 post-infection can predict long COVID. Early screening of depression helps health staff to identify patients at a higher risk of long COVID, allowing an early diagnosis of the condition.
Discovering the link between IL12RB1 gene polymorphisms and tuberculosis susceptibility: a comprehensive meta-analysis
Authors: Huang Jie , He Qiurong , Huang Lijun , Liu Liping , Yang Pei , Chen Min
Published: January 2024

Introduction

Numerous studies suggest that the risk of tuberculosis (TB) is linked to gene polymorphisms of the interleukin-12 receptor b subunit 1 (IL12RB1), but the association between IL12RB1 polymorphisms and TB susceptibility has not been thoroughly investigated.

Methods

A meta-analysis was conducted based on eight case-control studies with 10,112 individuals to further explore this topic. A systematic search of PubMed, Web of Science, Excerpt Medica Database, and Google Scholar up until April 6th, 2023 was performed. ORs and 95% CIs were pooled using the random-effect model. The epidemiological credibility of all significant associations was assessed using the Venice criteria and false-positive report probability (FPRP) analyses.

Results

The IL12RB1 rs11575934 and rs401502 showed solid evidence of no significant association with TB susceptibility. However, a weak association was observed between the IL12RB1 rs375947 biomarker and pulmonary tuberculosis (PTB) susceptibility (OR = 1.64, 95% CI: 1.22, 2.21).

Discussion

These findings should be confirmed through larger, better-designed studies to clarify the relationship between biomarkers in IL12RB1 gene and different types of TB susceptibility.
Co-occurrence of bacteria and viruses and serotype distribution of Streptococcus pneumoniae in the nasopharynx of Tanzanian children below 2 years of age following introduction of the PCV13
Authors: Emgård Matilda , Andersson Maria , Gonzales-Siles Lucia , Msuya Sia E. , Nyombi Balthazar M. , Nordén Rickard , Muro Florida , Lindh Magnus , Andersson Rune , Skovbjerg Susann
Published: January 2024

Introduction

Pneumococcal conjugate vaccines have reduced severe disease attributed to vaccine-type pneumococci in children. However, the effect is dependent on serotype distribution in the population and disease development may be influenced by co-occurrence of viral and bacterial pathogens in the nasopharynx.

Methods

Following introduction of the 13-valent pneumococcal conjugate vaccine (PCV13) in Tanzania we performed repeated cross-sectional surveys, including 775 children below 2 years of age attending primary healthcare centers. All children were sampled from nasopharynx and pneumococci were detected by single-target PCR. Pneumococcal serotypes/groups and presence of viruses and other bacteria were determined by two multiplex PCR assays.

Results

The prevalence of PCV13 vaccine-type pneumococci decreased by 50%, but residual vaccine-types were still detected in 21% of the children 2 years after PCV13 introduction. An increase in the non-vaccine-type 15 BC was observed. Pneumococci were often co-occurring with Haemophilus influenzae, and detection of rhino/enterovirus was associated with higher pneumococcal load.

Discussion

We conclude that presence of residual vaccine-type and emerging non-vaccine-type pneumococci in Tanzanian children demand continued pneumococcal surveillance. High co-occurrence of viral and bacterial pathogens may contribute to the disease burden and indicate the need of multiple public health interventions to improve child health in Tanzania.
Development and user evaluation of an immersive light field system for space exploration
Authors: Goriachev Vladimir , Kuula Timo , Tikka Petri , Karjalainen Jaakko , Helin Kaj
Published: January 2024

This paper presents the developmental work and user evaluation results of an immersive light field system built for the European Space Agency’s (ESA) project called “Light field-enhanced immersive teleoperation system for space station and ground control.” The main aim of the project is to evaluate the usefulness and feasibility of light fields in space exploration, and compare it to other types of immersive content, such as 360° photos and point clouds. In the course of the project, light field data were captured with a robotically controlled camera and processed into a suitable format. The light field authoring process was performed, and a light field renderer capable of displaying immersive panoramic or planar light fields on modern virtual reality hardware was developed. The planetary surface points of interest (POIs) were modeled in the laboratory environment, and three distinct test use cases utilizing them were developed. The user evaluation was held in the European Astronaut Centre (EAC) in the summer of 2023, involving prospective end-users of various backgrounds. During the evaluation, questionnaires, interviews, and observation were used for data collection. At the end of the paper, the evaluation results, as well as a discussion about lessons learned and possible improvements to the light field system, are presented.
Flexible Pressure and Temperature Microsensors for Textile-Integrated Wearables
Authors: dos Santos Dimitri Emmanuel , Queiroz José Bento , Garcia Inês Sofia , Vieira João , Fernandes José , Sotgiu Edoardo , Minas Graça , Bouçanova Maria , Arruda Luisa Mendes , Fangueiro Raul , Salgueiro-Oliveira Anabela , Ainla Alar , Serra Alves Filipe , Alves Dias Rosana
Published: January 2024

Environmental factors, such as pressure and temperature, are known to contribute to the formation of ulcers that seriously affect bedridden individuals. Researchers have proposed several technologies to achieve the long-term monitoring of those parameters, usually relying on sensing mats, which poses difficulties in correlating the measurements with specific parts of the body. In this work, we aim to develop microsensors to be integrated into patient clothing. They should be highly flexible, thin with a small footprint, and can be achieved by taking advantage of the microfabrication on polyimide (PI) thin-film substrates (total device thicknesses below 30 µm). Both resistive and capacitance transduction mechanisms were explored, targeting operation ranges of 1 to 40 kPa and 24 to 42 °C. The sensors were integrated into textiles using silicone elastomers and electrical connections based on conductive silver yarn. The experimental characterization showed a nominal capacitance of 21 pF, a sensitivity of −8.44 fF/kPa for the pressure sensors, and a 0.0021 Ω/Ω°C sensitivity of the temperature sensor (with resistance of 29 kΩ at 22 °C). The proposed approach can potentially be implemented not only in wearable devices but also in many other applications for health monitoring or human–machine interfaces.
Methods and Technologies for Supporting Knowledge Sharing within Learning Communities: A Systematic Literature Review
Authors: Zamiri Majid , Esmaeili Ali
Published: January 2024

In an era marked by swift technological advancements and an escalating emphasis on collaborative learning, understanding effective methods and technologies for sharing knowledge is imperative to optimize educational outcomes. This study delves into the varied methods and technologies applied to facilitate and support knowledge sharing within learning communities. To achieve this, a systematic literature review was conducted, systematically collecting and scrutinizing pertinent literature. Employing automated searches, title-based selection, and reputation-based filters ensured the inclusion of high-quality studies. The chosen studies underwent a meticulous evaluation, considering factors like relevance, methodological robustness, and currency. The literature review unveiled a diverse array of methods and technologies employed in learning communities to facilitate effective knowledge sharing. The outcomes of this study offer a comprehensive snapshot of the existing literature, underscoring the significance of methods and technologies in supporting knowledge sharing within learning communities. By comprehending the strengths, challenges, and potential future trajectories, educators, researchers, and policymakers can make informed decisions to enhance the efficacy of knowledge sharing within learning communities.
Exploratory Study of Romanian Generation Z Perceptions of Green Restaurants
Authors: Chiciudean Daniel I. , Shonkwiler Vanessa P. , Mureșan Iulia C. , Zaharia Alina , Chiciudean Gabriela O.
Published: January 2024

The hospitality industry has taken advantage of consumers’ increasing concerns regarding environmental issues. Worldwide, green restaurants have increased their profitability by using environmental attributes as a competitive advantage. In Romania, restaurants could profit from this new trend. This study aims to determine the perception of Generation Z consumers regarding green restaurants, since these younger consumers seem to have adopted more eco-friendly behavior compared to previous generations. Another objective of the study refers to assessing the importance of attributes relating to environmental protection and organic food when choosing a restaurant, consequently revealing a suitable market segment for this type of restaurant. In order to determine their degree of knowledge related to the concept of green restaurant, potential consuming behavior if implemented, and identifying the factors considered important when choosing a restaurant, this research compiles information from individuals belonging to Generation Z. Using principal component analysis, five factors were obtained, among which the “health and fresh food” was the most important. Based on the five dimensions, K-means cluster analysis led to three profiles of consumers. “Pro-environmental and convenience seekers” represent the largest cluster and should be considered the main target for future green restaurant practitioners. Additionally, our study finds an overall positive attitude toward green restaurants among the respondents and, more specifically, opinions on how these restaurants could address environmental issues and offer organic food, both important factors for Generation Z. This translates into a strong incentive for future entrepreneurs considering this business model in Romania.
Integrating Life Cycle Assessment in Conceptual Aircraft Design: A Comparative Tool Analysis
Authors: Mazur Kristina , Saleh Mischa , Hornung Mirko
Published: January 2024

Early and rapid environmental assessment of newly developed aircraft concepts is eminent in today’s climate debate. This can shorten the decision-making process and thus accelerate the entry into service of climate-friendly technologies. A holistic approach within the conceptual aircraft design is taken into consideration in terms of a life cycle assessment (LCA) to properly model and evaluate these concepts. To provide an understanding of how different LCA software affects the assessment, the goals of this study are to establish a baseline metrics definition for comparative evaluation and apply them to two tools. The first tool is an existing simplified derivative of openLCA, while the second, developed in this study, is an automated interface to the same software. The main finding is that researchers and practitioners must carefully consider the intended use of the tool. The simplified tool is suitable for training and teaching purposes and assessments on single score level. In contrast, an advanced tool is required in order to appropriately analyze the overall impact categories requiring high levels of LCA expertise, modeling, and time effort.
Farming of Medicinal and Aromatic Plants in Italy: Structural Features and Economic Results
Authors: Macaluso Dario , Licciardo Francesco , Carbone Katya
Published: January 2024

In recent years, the primary sector in Italy and elsewhere has been profoundly affected by climate change and a deep economic crisis, mainly linked to stagnating prices and rising production costs. Because of this situation, we are witnessing renewed interest in alternative agricultural productions, which are characterized by their resilience and sustainability, including medicinal and aromatic plants (MAPs). This sector is characterized by a certain heterogeneity due to the great variety of species and their wide range of uses. Although these characteristics contribute to the sector’s economic success, they also hinder its study due to commodity complexity and limited data availability. At the farm level, the situation is complicated by the fact that MAP cultivation is often embedded in complex cropping systems, and more rarely, is practiced exclusively or predominantly. In light of these considerations, we concentrated solely on the agricultural phase of the supply chain, using data available in the Farm Accountancy Data Network. We aimed to examine the main structural characteristics and economic outcomes of Italian farms that grow MAP, as well as the profitability of some of the species. To ensure accurate species classification, only MAPs exclusively designated for botanical use in the Italian National List were considered. The analysis of farm economic performance indicators (gross output, variable costs, gross margins, etc.) focused mainly on the species most represented in the sample: saffron, rosemary, lavender, oregano, and sage. The results indicate that the total gross output and gross margin show the best performance in the case of saffron (66,200 and 57,600 EUR/ha, respectively) and rosemary (27,500 and 22,000 EUR/ha, respectively). However, for saffron, the biggest cost concerns propagation (purchase of bulbs), amounting to 50% of the variable costs, whereas fertilization ones are particularly high for sage and rosemary.
Ecological and Economic Benefits of Greenhouse Gas Emission Reduction Strategies in Rice Production: A Case Study of the Southern Rice Propagation Base in Hainan Province
Authors: Zhang Xianxian , Bi Junguo , Wang Weikang , Sun Donglai , Sun Huifeng , Bi Qingyu , Wang Cong , Zhang Jining , Zhou Sheng , Luo Lijun
Published: January 2024

Developing tailored emission reduction strategies and estimating their potential is crucial for achieving low-carbon rice production in a specific region, as well as for advancing China’s dual carbon goals in the agricultural sector. By utilizing water-saving and drought-resistant rice (WDR) with enhanced water and nitrogen utilization efficiency, the mitigation strategies were constructed for rice production systems, and their potential for emission reduction was estimated in the southern rice propagation base of Hainan Province. This study revealed that the implementation of a reduction strategy, which involves dry direct seeding and dry cultivation, combined with a 53% reduction in nitrogen fertilizer, can effectively synergize the mitigation of methane (CH4) and nitrous oxide (N2O) emissions from rice paddies. Compared with traditional flooded rice cultivation, this integrated approach exhibits an impressive potential for reducing net greenhouse gas (GHG) emissions by 97% while simultaneously doubling economic benefits. Moreover, when combined with plastic film mulching, the strategy not only sustains rice yields but also achieves a remarkable emission reduction of 92%, leading to a fourfold increase in economic benefits. Our study provides a comprehensive low-carbon sustainable development strategy for rice production in the southern rice propagation base of Hainan Province and offers valuable insights for researching GHG emissions in other regions or crops. These emission reduction pathways and the assessment method could contribute to the realization of low-carbon agriculture.
Characteristics of N Transformation of Humic Acid Urea in Different Circle Layers of the Fertisphere: A Simulated Experiment
Authors: Liu Min , Xu Meng , Yuan Liang , Zhang Shuiqin , Li Yanting , Zhao Bingqiang
Published: January 2024

Due to its broad yield-increasing effect and low cost, humic acid urea (HAU) has become one of the leading modified fertilizers worldwide. The fertisphere is the primary space where urea (U) granules participate in the soil nitrogen cycle, forming a nutrient concentration gradient centered on the point of fertilization. The closer the circle layers to the urea granule in the fertisphere, the higher the nitrogen concentration. However, HAU in this microregion remains poorly understood. The differences in the transformation process from the inside to outside circle layers of the U and HAU fertispheres were simulated and studied using soil incubation experiments under 20, 10, 2, 1, and 0.2 g kg−1 nitrogen inputs. The 20 and 10 g kg−1 inputs represent the layers closest to the urea granule. Within the first seven days, HAU treatment showed higher concentrations of soil ammonia-N content than U treatment within the two layers closest to the fertilizer core, while exhibiting lower concentrations under the farthest two layers. Under 2 g kg−1 nitrogen input, the nitrate nitrogen under the HAU treatment was significantly higher than that in the U treatment, indicating a higher nitrification rate. During the 42-day incubation period, soil mineral nitrogen content under the HAU treatment was higher than that for the U treatment in the two closest circles. On the 42nd day, the residual urea-N under the HAU treatment was significantly higher than that for the U treatment when the nitrogen input was higher than 1 g kg−1. The effect of higher fertilizer preservation and supply capacity of HAU in Fluvo-aquic soil was achieved by changing the urease activity and nitrification rate in fertisphere ranges closer to the fertilizer core. An improved understanding of the high-efficiency mechanism of HAU in the fertisphere process will contribute to the development of new-generation high-efficiency urea products.
Effects of Pre-Germinative Treatments and Temperatures on Tassel Hyacinth [Muscari comosum (L.) Mill.] Seeds
Authors: Castronuovo Donato , Cardone Loriana , Candido Vincenzo
Published: January 2024

Muscari comosum (L.) Mill. is a spontaneous plant that grows in the whole Mediterranean area, including the Basilicata and Puglia regions (southern Italy), where it has received inclusion in the Italian National List for Traditional Agri-Food Product (TAP). The food and medicinal uses of bulb are ancient due to its antioxidant properties and high variety of nutrients, such as starch, sugars, and minerals. Muscari seed is characterized by morpho-physiological dormancy, and in order to achieve uniform germination, some pre-germinative treatments are needed. In this research, the effects of hydro-priming and osmo-priming, i.e., PEG 8000 and KNO3, as well as three germination temperatures (4, 10, and 20 °C), have been evaluated. In general, the average results pointed out that the pre-treatments increased the germination index (GI) by 5% and the germination percentage (GP) by 3% compared to the no-primed control. The germination temperature of 10 °C significantly reduced the median germination time (T50) by 5.4 days and the mean germination time (MGT) by 5 days compared to temperature at 4 °C. In particular, the best results were obtained by “hydro-priming treatment × 10 °C” interaction, in terms of T50 (34.9 days) and MGT (36.3 days). This combination decreased the T50 by 10.5 days and the MGT by 9.6 days compared to the “control × 4 °C” interaction. Pearson’s correlation matrix results highlighted a significant positive link between T50 and MGT (r = 0.993). In conclusion, these techniques enhanced the germination potential so that the use of pre-treated seeds could be included in a cultivation protocol to improve the germination phase and satisfy the growing demand for Italian bulbs.
Detection and Analysis of Chili Pepper Root Rot by Hyperspectral Imaging Technology
Authors: Shao Yuanyuan , Ji Shengheng , Xuan Guantao , Ren Yanyun , Feng Wenjie , Jia Huijie , Wang Qiuyun , He Shuguo
Published: January 2024

The objective is to develop a portable device capable of promptly identifying root rot in the field. This study employs hyperspectral imaging technology to detect root rot by analyzing spectral variations in chili pepper leaves during times of health, incubation, and disease under the stress of root rot. Two types of chili pepper seeds (Manshanhong and Shanjiao No. 4) were cultured until they had grown two to three pairs of true leaves. Subsequently, robust young plants were infected with Fusarium root rot fungi by the root-irrigation technique. The effective wavelength for discriminating between distinct stages was determined using the successive projections algorithm (SPA) after capturing hyperspectral images. The optimal index related to root rot between each normalized difference spectral index (NDSI) was obtained using the Pearson correlation coefficient. The early detection of root rot illness can be modeled using spectral information at effective wavelengths and in NDSI, together with the application of partial least squares discriminant analysis (PLS-DA), least squares support vector machine (LSSVM), and back-propagation (BP) neural network technology. The SPA-BP model demonstrates outstanding predictive capabilities compared with other models, with a classification accuracy of 92.3% for the prediction set. However, employing SPA to acquire an excessive number of efficient wave-lengths is not advantageous for immediate detection in practical field scenarios. In contrast, the NDSI (R445, R433)-BP model uses only two wavelengths of spectral information, but the prediction accuracy can reach 89.7%, which is more suitable for rapid detection of root rot. This thesis can provide theoretical support for the early detection of chili root rot and technical support for the design of a portable root rot detector.
Improve the Simulation of Radiation Interception and Distribution of the Strip-Intercropping System by Considering the Geometric Light Transmission
Authors: Dong Liming , Lu Yuchao , Lei Guoqing , Huang Jiesheng , Zeng Wenzhi
Published: January 2024

Intercropping radiation interception model is a promising tool for quantifying solar energy utilization in the intercropping system. However, few models have been proposed that can simulate intercropping radiation interception accurately and with simplicity. This study proposed a new statistical model (DRT model), which enables the simulation of daily radiation distribution by considering the geometric light transmission in the intercropping system. To evaluate model performance, the radiation interception and distribution in two wheat/maize strip intercropping experiments (A and B) were simulated with the DRT model and other two statistical models, including the horizontal homogeneous canopy model (HHC model) and the Gou Fang model (GF model). Experiment A was conducted in different intercropping configurations, while Experiment B was conducted in soils with different salinity levels. In both experiments, the HHC model exhibited the poorest performance (0.120 < RMSE < 0.172), while the DRT model obtained a higher simulation accuracy in the fraction of photosynthetically active radiation (PAR) interception, with RMSE lower by 0.008–0.022 and 0.022–0.125 than the GF and the HHC models, respectively. Especially, the DRT model showed stronger stability than the other two models under soil salinity stress, with R2 higher by 0.129–0.354 and RMSE lower by 0.011–0.094. Moreover, the DRT model demonstrated a relatively ideal simulation of the daily radiation distribution in Experiment A (0.840 < R2 < 0.893, 0.105 < RMSE < 0.140) and Experiment B (0.683 < R2 < 0.772, 0.111 < RMSE < 0.143), especially when the continuous canopy formed during the later crop growth stages. These results indicate the superiority of the DRT model and could improve our understanding of radiation utilization in the intercropping system.
Effects of Different Proportions of Organic Fertilizer Replacing Chemical Fertilizer on Soil Nutrients and Fertilizer Utilization in Gray Desert Soil
Authors: Lu Weidan , Hao Zhiqiang , Ma Xiaolong , Gao Jianglong , Fan Xiaoqin , Guo Jianfu , Li Jianqiang , Lin Ming , Zhou Yuanhang
Published: January 2024

Organic fertilizer can improve soil management and alleviate soil nutrient loss caused by excessive fertilization. This study determines a fertilization scheme that can achieve high and stable crop yield and effective soil fertilization by exploring the effects of different organic fertilizer proportions on soil nutrient content, fertilizer utilization rate, and wheat yield. The experiment was conducted from 2018 to 2020 using the Xinchun 38 wheat variety and gray desert soil. The experiment used six treatments: no fertilizer (CK), with normal nitrogen and phosphorus fertilizer (CF). The amount of conventional chemical nitrogen fertilizer was reduced by 6%, 12%, 18%, and 24%, respectively, and supplemented with organic nitrogen fertilizer of the same proportion. The experimental treatment codes were SF6, SF12, SF18, and SF24, respectively. Plant samples from six wheat growth stages and 0–20 cm soil samples were collected to analyze the nitrogen and phosphorus content and organic matter; dry matter accumulation, crop yield, and yield components were measured at the harvest stage. Soil available nitrogen, phosphorus, potassium, and soil organic matter contents increased with the replacement ratio of organic fertilizer after three consecutive years of application. The available nutrients and organic matter in soil treated with SF18 and SF24 were significantly higher than those in CK and CF. The dry matter accumulation and nutrient accumulation of wheat increased with increasing organic fertilizer replacement rates of SF18 and SF24, respectively. Nitrogen and phosphorus fertilizer utilization rates, partial productivity, and agricultural use efficiency also increased, with the SF18 and SF24 treatments having higher organic fertilizer replacement rates than those in the other treatments. The number of ears, 1000-grain weight, and yield of wheat treated with SF18 treatment produced the best results. Therefore, continuously using organic fertilizer as a partial replacement for conventional fertilizer can increase wheat growth and soil nutrient availability, which can improve the utilization rate of fertilizer, thereby achieving stability and even a significant increase in yield.
The Effects of Soil Salt Stress on the Nitrogen Uptake, Yield Response and Nitrogen Use Efficiency of Cotton in Arid Areas
Authors: Heng Tong , Ma Yingjie , Ai Pengrui , Liu Zhenyue , Wu Miao , Liu Changjiang
Published: January 2024

Soil salinity is a major soil threat, causing severe environmental problems. Soil salt stress limits N uptake and cotton growth, especially in arid regions. However, the mechanism underlying stress tolerance in cotton plants under different soil salinity levels has not been fully elucidated. Therefore, the aim of this study was to examine the proportion and mechanism of cotton N uptake and transport under salt stress using the 15N isotope labeling technique. Cotton plants were grown in four undisturbed loamy soils (CK, non-salinity, <2 dS m−1; C1, low salinity, 2–4 dS m−1; C2, mid-salinity, 4–8 dS m−1; and C3, high salinity, 8–15 dS m−1) in the test pit experiments. The findings indicated that the cotton N uptake was limited by the soil salt stress and total N content, and the cotton 15N use efficiency decreased by 25–27% with increasing salinity (>4.0 dS m−1). The N transported to cotton from the 0–20 cm soil layer in C1 treatment was higher than from the 40–60 cm layer. Furthermore, the cotton fruit cultivated in C1 exhibited a notably elevated dry matter content compared to those cultivated in the control treatment. As the soil salt stress increased from the control treatment to C1, the cotton stems and fruits demonstrated a gradual enhancement in their N uptake and regulatory capabilities, albeit with a slight decrease observed in the leaves. These findings emphasize that soil salt stress diminishes cotton nitrogen uptake and transport, potentially exacerbating nitrogen pollution in the absence of optimized nitrogen fertilization.
Arbuscular Mycorrhizal Fungi as Biofertilizers to Increase the Plant Quality of Sour-Orange Seedlings
Authors: Navarro Josefa María , Morte Asunción
Published: January 2024

In addressing the agricultural challenges posed by climate change, the use of biofertilizers, derived from living organisms, promotes environmentally friendly crop cultivation, and represents an adaptive strategy for sustainable agriculture in the face of climate uncertainty. Careful selection of the arbuscular mycorrhizal fungus (AMF) would represent a crucial step in mycorrhizal inoculation, considering the varying levels of compatibility between the AMF and the host plant. This study aimed to assess the impact of two AMF species that are prevalent in citrus soils of south-eastern Spain (Rhizophagus irregularis and Funneliformis mosseae) on the Citrus aurantium seedlings’ behavior. Sour-orange plants showed a high mycorrhizal dependence regardless of the specific AMF species. Both R. irregularis and F. mosseae fungi exhibited high colonization percentages, with R. irregularis outperforming F. mosseae in root colonization. Inoculation with both AMF yielded notable growth improvements, but R. irregularis exhibited higher positive effects in the long term. The heightened P nutrition and increased chlorophyll concentration significantly enhanced the performance of AMF-inoculated plants. With F. mosseae, plants showed more pronounced improvements in P nutrition and a stronger correlation of their dry mass with P concentration; however, in general, inoculation with R. irregularis produced a higher sour-orange-plant performance. Both R. irregularis and F. mosseae fungi produced strong positive effects in sour-orange growth, which positioned them as viable biofertilizer options. These results can contribute to enhancing understanding for the development of an improved design of biofertilizers used in regions that are vulnerable to climate change, such as south-eastern Spain. This promotes a shift towards more sustainable and environmentally friendly agricultural practices by reducing dependence on chemical fertilizers.
The Role of Early Rehabilitation and Functional Electrical Stimulation in Rehabilitation for Cats with Partial Traumatic Brachial Plexus Injury: A Pilot Study on Domestic Cats in Portugal
Authors: Gouveia Débora , Cardoso Ana , Carvalho Carla , Rijo Inês , Almeida António , Gamboa Óscar , Lopes Bruna , Sousa Patrícia , Coelho André , Balça Maria Manuel , Salgado António J. , Alvites Rui , Varejão Artur Severo P. , Maurício Ana Colette , Ferreira António , Martins Ângela
Published: January 2024

This prospective observational cohort pilot study included 22 cats diagnosed with partial traumatic brachial plexus injury (PTBPI), aiming to explore responses to an early intensive neurorehabilitation protocol in a clinical setting. This protocol included functional electrical stimulation (FES), locomotor treadmill training and kinesiotherapy exercises, starting at the time with highest probability of nerve repair. The synergetic benefits of this multimodal approach were based on the potential structural and protective role of proteins and the release of neurotrophic factors. Furthermore, FES was parametrized according to the presence or absence of deep pain. Following treatment, 72.6% of the cats achieved ambulation: 9 cats within 15 days, 2 cats within 30 days and 5 cats within 60 days. During the four-year follow-up, there was evidence of improvement in both muscle mass and muscle weakness, in addition to the disappearance of neuropathic pain. Notably, after the 60 days of neurorehabilitation, 3 cats showed improved ambulation after arthrodesis of the carpus. Thus, early rehabilitation, with FES applied in the first weeks after injury and accurate parametrization according to the presence or absence of deep pain, may help in functional recovery and ambulation, reducing the probability of amputation.
A Thorny Tale of Parasites: Screening for Enteric Protozoan Parasites in Hedgehogs from Portugal
Authors: Gomes-Gonçalves Sara , Santos-Silva Sérgio , Cruz Andreia V. S. , Rodrigues Clarisse , Soeiro Vanessa , Barradas Patrícia , Mesquita João R.
Published: January 2024

Enteric protozoan parasites, such as Blastocystis sp., Balantioides coli, Cryptosporidium spp., and Giardia duodenalis, may have implications for both animal and human health.Transmitted through the fecal–oral route, these parasites cause symptoms such as diarrhea, abdominal pain, and weight loss. This study investigated the presence of these enteric protozoan parasites and genetically characterized them in hedgehogs from Portugal. A total of 110 hedgehog stool samples were collected. Molecular detection methods showed an overall occurrence of protozoa in 1.82% (2/110 95% CI: 0.22–6.41) of hedgehogs, with Blastocystis being found in one hedgehog and Cryptosporidium being found in another. No evidence for the presence of B. coli or G. duodenalis was found. This study suggests that there is a need to stay aware of hedgehogs as potential hosts of enteric protozoa. Ongoing research and surveillance efforts are recommended to explore practical prevention and control strategies. The results contribute to the limited knowledge of these parasites in Portuguese hedgehog populations and underscore their potential relevance to both veterinary and public health.
Knowledge Gaps on the Utilization of Fossil Shell Flour in Beef Production: A Review
Authors: Soji-Mbongo Zimkhitha , Mpendulo Thando Conference
Published: January 2024

Population growth in many countries results in increased demand for livestock production and quality products. However, beef production represents a complex global sustainability challenge, including meeting the increasing demand and the need to respond to climate change and/or greenhouse gas emissions. Several feed resources and techniques have been used but have some constraints that limit their efficient utilization which include being product-specific, not universally applicable, and sometimes compromising the quality of meat. This evokes a need for novel techniques that will provide sustainable beef production and mitigate the carbon footprint of beef while not compromising beef quality. Fossil shell flour (FSF) is a natural additive with the potential to supplement traditional crops in beef cattle rations in response to this complex global challenge as it is cheap, readily available, and eco-friendly. However, it has not gained much attention from scientists, researchers, and farmers, and its use has not yet been adopted in most countries. This review seeks to identify knowledge or research gaps on the utilization of fossil shell flour in beef cattle production, with respect to climate change, carcass, and meat quality. Addressing these research gaps would be a step forward in developing sustainable and eco-friendly beef production.
The Cetacean Sanctuary: A Sea of Unknowns
Authors: Bruck Jason N.
Published: January 2024

Housing cetaceans in netted sea pens is not new and is common for many accredited managed-care facilities. Hence, the distinction between sanctuary and sea pen is more about the philosophies of those who run these sanctuary facilities, the effects of these philosophies on the animals’ welfare, and how proponents of these sanctuaries fund the care of these animals. Here, I consider what plans exist for cetacean sanctuaries and discuss the caveats and challenges associated with this form of activist-managed captivity. One goal for stakeholders should be to disregard the emotional connotations of the word “sanctuary” and explore these proposals objectively with the best interest of the animals in mind. Another focus should be related to gauging the public’s understanding of proposed welfare benefits to determine if long-term supporters of donation-based sanctuary models will likely see their expectations met as NGOs and their government partners consider moving forward with cetacean sanctuary experiments.
Prevalence and Risk Factors Associated with Rib Lesions in Dairy Cows
Authors: Stilwell George , Azevedo João M. B. , Nunes Telmo
Published: January 2024

Unilateral or bilateral hard bony enlargement is sometimes palpated over dairy cows’ ribs. Although usually not painful, these injuries may have welfare and economic implications and can be used as indicators of poor husbandry conditions. The objective of this study was to determine the prevalence of rib lesions in dairy cows kept in intensive systems and to identify the risk factors either associated with the housing or with the animal’s clinical history. We examined the ribs of all 1319 lactating cows from 22 Portuguese dairy farms. In each farm, housing and animal potential risk factors were recorded. Additionally, clinical examination, including lameness scoring, was performed on all cows showing rib lesions. The global rib lesions’ prevalence per farm was 2.3%, ranging from 0% (n = 12 farms) to 6.1% (n = 1 farm). Lesions were found in cows with an average of 3.7 lactations. The main farm risk factors were narrow stalls; insufficient feeding places; insufficient cubicles; and the presence of an exposed curb at the back end of the stalls. Rib lesions were associated with a history of lameness but not with lame cows at the moment of examination or a history of being a downer cow. In conclusion, rib lesions’ prevalence can be high in some farms, being significantly associated with cubicle design and lameness. By being associated with chronic lameness and inadequate housing, rib lesions should be included in dairy cows’ welfare assessment protocols.