bluuebunny/crossref_metadata_2025 huggingface dataset:
Facile Green Synthesis of Zinc Oxide Nanoparticles with Potential Synergistic Activity with Common Antifungal Agents against Multidrug-Resistant Candidal Strains
Authors: Yassin Mohamed Taha , Mostafa Ashraf Abdel-Fattah , Al-Askar Abdulaziz Abdulrahman , Al-Otibi Fatimah O.
Published: May 2022

The high incidence of fungal resistance to antifungal drugs represents a global concern, contributing to high levels of morbidity and mortality, especially among immunocompromised patients. Moreover, conventional antifungal medications have poor therapeutic outcomes, as well as possible toxicities resulting from long-term administration. Accordingly, the aim of the present study was to investigate the antifungal effectiveness of biogenic zinc oxide nanoparticles (ZnO NPs) against multidrug-resistant candidal strains. Biogenic ZnO NPs were characterized using physicochemical methods, such as UV-vis spectroscopy, transmission electron microscopy (TEM), energy-dispersive X ray (EDX) spectroscopy, FTIR (Fourier transform infrared) spectroscopy and X-ray powder diffraction (XRD) analysis. UV spectral analysis revealed the formation of two absorption peaks at 367 and 506 nm, which preliminarily indicated the successful synthesis of ZnO NPs, whereas TEM analysis showed that ZnO NPs exhibited an average particle size of 22.84 nm. The EDX spectrum confirmed the successful synthesis of ZnO nanoparticles free of impurities. The FTIR spectrum of the biosynthesized ZnO NPs showed different absorption peaks at 3427.99, 1707.86, 1621.50, 1424.16, 1325.22, 1224.67, 1178.22, 1067.69, 861.22, 752.97 and 574.11 cm−1, corresponding to various functional groups. The average zeta potential value of the ZnO NPs was −7.45 mV. XRD analysis revealed the presence of six diffraction peaks at 2θ = 31.94, 34.66, 36.42, 56.42, 69.54 and 76.94°. The biogenic ZnO NPs (100 µg/disk) exhibited potent antifungal activity against C. albicans, C. glabrata and C. tropicalis strains, with suppressive zone diameters of 24.18 ± 0.32, 20.17 ± 0.56 and 26.35 ± 0.16 mm, respectively. The minimal inhibitory concentration (MIC) of ZnO NPs against C. tropicalis strain was found to be 10 μg/mL, whereas the minimal fungicidal concentration (MFC) was found to be 20 μg/mL. Moreover, ZnO NPs revealed a potential synergistic efficiency with fluconazole, nystatin and clotrimazole antifungal drugs against C. albicans strain, whereas terbinafine, nystatin and itraconazole antifungal drugs showed a potential synergism with ZnO NPs against C. glabrata as a multidrug-resistant strain. In conclusion, pomegranate peel extract mediated green synthesis of ZnO NPs with potential physicochemical features and antimicrobial activity. The biosynthesized ZnO NPs could be utilized for formulation of novel drug combinations to boost the antifungal efficiency of commonly used antifungal agents.
Fuzzy Approach for Reliability Modeling of Lead-Free Solder Joints in Elevated Temperature Environmental Conditions
Authors: Al Athamneh Raed , Abueed Mohammed , Bani Hani Dania , Hamasha Sa’d
Published: May 2022

The mechanical and fatigue properties of microelectronic interconnection materials are important issues in critical applications in the life of electronic assemblies. Due to the growth in the applications of electronic components in new technological products used in tough conditions, evaluating the reliability of solder alloys has become crucial to the prediction of product life. SAC (Sn-Ag-Cu) solder alloys are common lead-free alloys used to form solder joints. In the current study, the reliability of individual SAC305 solder joints in actual setting conditions with an organic solderability preservative (OSP) surface finish was examined using an accelerated shear fatigue test at different load levels (16 MPa, 20 MPa, and 24 MPa). Four operating temperature levels (−10, 25, 60, and 100 °C) were also used. Seven samples were utilized at each experimental condition. An orthogonal array (L12) was employed for this experiment. The fatigue behavior of the SAC305 solder joints in actual setting conditions was described by a two-parameter Weibull distribution. The characteristic life and the shape parameter were extrapolated from the Weibull distribution for each experimental condition. Characteristic life was employed to represent the fatigue resistance of the solder joints. For each sample, the inelastic work per cycle and plastic deformation were determined. The results indicated a notable increase in the inelastic work per cycle and the plastic strain as a result of increasing either the stress load or the working temperature. Combinations of stress amplitude, working temperature, inelastic work, and plastic deformation were applied as inputs to the fuzzy system for computing a comprehensive output measure (COM-Value) using the Mamdani method. A negative relationship was observed between the solder life and the four fuzzy inputs. To fuzzify the inputs of the fuzzy system, two membership functions, “MFs”, were formed for each input, and five MFs were set for the output. The center-of-gravity (COG) theorem was utilized as a defuzzification method in the fuzzy inference system. The characteristic life of the solder joints was predicted by the COM-Value, which was used as an independent variable. Finally, The COM-Value was used to generate a general predicted reliability model for SAC305 solder joints with an acceptable model adequacy index.
Imaging Features of Post Main Hepatectomy Complications: The Radiologist Challenging
Authors: Cutolo Carmen , De Muzio Federica , Fusco Roberta , Simonetti Igino , Belli Andrea , Patrone Renato , Grassi Francesca , Dell’Aversana Federica , Pilone Vincenzo , Petrillo Antonella , Izzo Francesco , Granata Vincenza
Published: May 2022

In the recent years, the number of liver resections has seen an impressive growth. Usually, hepatic resections remain the treatment of various liver diseases, such as malignant tumors, benign tumors, hydatid disease, and abscesses. Despite technical advancements and tremendous experience in the field of liver resection of specialized centers, there are moderately high rates of postoperative morbidity and mortality, especially in high-risk and older patient populations. Although ultrasonography is usually the first-line imaging examination for postoperative complications, Computed Tomography (CT) is the imaging tool of choice in emergency settings due to its capability to assess the whole body in a few seconds and detect all possible complications. Magnetic resonance cholangiopancreatography (MRCP) is the imaging modality of choice for delineating early postoperative bile duct injuries and ischemic cholangitis that may arise in the late postoperative phase. Moreover, both MDCT and MRCP can precisely detect tumor recurrence. Consequently, radiologists should have knowledge of these surgical procedures for better comprehension of postoperative changes and recognition of the radiological features of various postoperative complications.
Breast Cancer Screening Based on Supervised Learning and Multi-Criteria Decision-Making
Authors: Mustapha Mubarak , Ozsahin Dilber , Ozsahin Ilker , Uzun Berna
Published: May 2022

On average, breast cancer kills one woman per minute. However, there are more reasons for optimism than ever before. When diagnosed early, patients with breast cancer have a better chance of survival. This study aims to employ a novel approach that combines artificial intelligence and a multi-criteria decision-making method for a more robust evaluation of machine learning models. The proposed machine learning techniques comprise various supervised learning algorithms, while the multi-criteria decision-making technique implemented includes the Preference Ranking Organization Method for Enrichment Evaluations. The Support Vector Machine, having achieved a net outranking flow of 0.1022, is ranked as the most favorable model for the early detection of breast cancer. The net outranking flow is the balance between the positive and negative outranking flows. This indicates that the higher the net flow, the better the alternative. K-nearest neighbor, logistic regression, and random forest classifier ranked second, third, and fourth, with net flows of 0.0316, −0.0032, and −0.0541, respectively. The least preferred alternative is the naive Bayes classifier with a net flow of −0.0766. The results obtained in this study indicate the use of the proposed method in making a desirable decision when selecting the most appropriate machine learning model. This gives the decision-maker the option of introducing new criteria into the decision-making process.
Are Experts Well-Calibrated? An Equivalence-Based Hypothesis Test
Authors: Dharmarathne Gayan , Hanea Anca M. , Robinson Andrew
Published: May 2022

Estimates based on expert judgements of quantities of interest are commonly used to supplement or replace measurements when the latter are too expensive or impossible to obtain. Such estimates are commonly accompanied by information about the uncertainty of the estimate, such as a credible interval. To be considered well-calibrated, an expert’s credible intervals should cover the true (but unknown) values a certain percentage of time, equal to the percentage specified by the expert. To assess expert calibration, so-called calibration questions may be asked in an expert elicitation exercise; these are questions with known answers used to assess and compare experts’ performance. An approach that is commonly applied to assess experts’ performance by using these questions is to directly compare the stated percentage cover with the actual coverage. We show that this approach has statistical drawbacks when considered in a rigorous hypothesis testing framework. We generalize the test to an equivalence testing framework and discuss the properties of this new proposal. We show that comparisons made on even a modest number of calibration questions have poor power, which suggests that the formal testing of the calibration of experts in an experimental setting may be prohibitively expensive. We contextualise the theoretical findings with a couple of applications and discuss the implications of our findings.
Selected Environmental Impact Indicators Assessment of Wind Energy in India Using a Life Cycle Assessment
Authors: Verma Shalini , Paul Akshoy Ranjan , Haque Nawshad
Published: May 2022

This study focuses on the life cycle assessment (LCA) of an onshore wind farm in India. The study is conducted on 10 Vestas 1.65 MW wind turbines situated in the Karnataka state of India. Following the ISO 14044 standard, SimaPro LCA software is used to model the process. The functional unit is chosen as 1 MWh sent out electricity. The results of the life cycle-based emissions of wind farm are compared with those of the coal power plant. The global warming potential is found to be 11.3 g CO2-eq/MWh for wind power, which is 98.8% lower than that for the coal power plant. A comparison of data available in SimaPro LCA software was carried out with data in GaBi software. There is a small difference between the two databases. This may be due to different boundary and inclusion of input items. Steel, aluminium, and concrete contributed 86%, 84%, 84% and 85% of total CO2, NOx, SO2 and PM2.5 emissions, respectively. Recycling the materials of a wind turbine at the end of its life can reduce the environmental impact. Higher capacity factors can increase the electricity generation with reduced environmental impact. A 22% increase in capacity factor can reduce environmental impact by 19%. In addition, the increase in the life of wind turbines reduces the environmental impact, as a wind turbine only has a few moving parts and requires minimum regular maintenance.
Development of a High Perfomance Gas Thermoelectric Generator (TEG) with Possibible Use of Waste Heat
Authors: Dashevsky Zinovi , Jarashneli Albert , Unigovski Yaakov , Dzunzda Bohdan , Gao Feng , Shneck Roni
Published: May 2022

A huge concern regarding global warming, as well as the depletion of natural fuel resources, has led to a wide search for alternative energy sources. Due to their high reliability and long operation time, thermoelectric generators are of significant interest for waste heat recovery and power generation. The main disadvantage of TEGs is the low efficiency of thermoelectric commercial modules. In this work, a unique design for a multilayer TE unicouple is suggested for an operating temperature range of 50–600 °C. Two types of thermoelectric materials were selected: «low temperature» n-and p-type TE materials (for the operating temperature range of 50–300 °C) based on Bi2Te3 compounds and «middle temperature» (for the operating temperature range of 300–600 °C) n- and p-type TE materials based on the PbTe compound. The hot extrusion technology was applied to fabricate n- and p-type low-temperature TE materials. A unique design of multilayer TEG was experienced to achieve an efficiency of up to 15%. This allows for the possibility of extracting this amount of electrical power from the heat generated for domestic and water heating.
Machine Learning in Power System Dynamic Security Assessment
Authors: Sarajcev Petar
Published: May 2022

Recent growing energy crisis in Europe, coupled with the rising energy prices worldwide, is a clear indication of the many difficulties awaiting the transition of modern societies away from fossil fuels [...]
Tree Fresh Leaf- and Twig-Leached Dissolved Organic Matter Quantity and Biodegradability in Subtropical Plantations in China
Authors: Xu Jia-Wen , Ji Jing-Hao , Hu Dong-Nan , Zheng Zhi , Mao Rong
Published: May 2022

Extreme weather events often cause the input of fresh plant tissues into soils in forests. However, the interspecific patterns of tree fresh plant tissue-leached dissolved organic matter (DOM) characteristics are poorly understood. In this study, we collected fresh leaves and twigs of two broadleaf trees (Liquidambar formosana and Schima superba) and two coniferous trees (Pinus massoniana and Pinus elliottii) in subtropical plantations in China, and measured tree fresh tissue-leached DOM quantity and biodegradability. The interspecific patterns of fresh plant tissue-leached DOM production varied with organ types. Broadleaf tree leaves leached greater amounts of dissolved organic carbon (DOC), dissolved total nitrogen (DTN), and dissolved total phosphorus (DTP) than coniferous tree leaves, but an opposite pattern of DOC and DTN productions was observed between broadleaf and coniferous tree twigs. Regardless of tree species, leaves often leached greater quantities of DOC, DTN, and DTP than twigs. For both leaves and twigs, broadleaf tree tissue-leached DOM had greater aromaticity and lower biodegradability than coniferous tree tissue-leached DOM. Moreover, leaf-leached DOM had greater aromaticity and lower biodegradability than twig-leached DOM. In addition, DOM biodegradability negatively correlated with the initial aromaticity and DOC:DTN ratio, despite no relationship between DOM biodegradability and DOC:DTP ratio. These findings highlight the pivotal roles of leaf habit and organ type in regulating fresh tree tissue-leached DOM production and biodegradability and reveal that the substantial variations of fresh tissue-leached DOM biodegradability are co-driven by DOM aromaticity and N availability in subtropical plantations in China.
An Electric Signal Conduction Characterization Model (ESCCM) for Establishing an Effective Poplar Regenerative System
Authors: Zhang Yue , Li Qing , Sen Meng , Han Xiao , Wang Xiaoling , Zhou Yangyan
Published: May 2022

The poplar is a model system for research on wood plant biology. An establishment of an efficient poplar regeneration system (PRS) plays a key role in the molecular breeding of wood plants. At present, most established PRSs are based on orthogonal experiments of previous research data. However, such an experiment is complex, time-consuming, and inefficient for various poplar subspecies. Therefore, an efficient solution to the establishment of PRSs is urgent. In this study, the triploid white poplar (Populus tomentosa ‘YiXianCiZhu B385′) was used as an experimental material to establish a leaf-based regeneration system. Firstly, different concentrations of hormones were added into the medium for the differentiation, stretching, and rooting of leaves, and the electrical conductivity of the medium was measured by a conductivity meter. Secondly, the optimal hormone concentrations for differentiation, stretching, and rooting were obtained by wavelet analysis. Finally, the Electrical Signal Conduction Characterization Model (ESCCM) of different hormone concentrations in the differentiation, stretching, and rooting of poplars was established. The result showed that the ESCCM improves the efficiency of PRSs, and this provides new insight and theory in molecular breeding. The ESCCM also provides the possibility of an automated establishment of a PRS.
Accretion of Galaxies around Supermassive Black Holes and a Theoretical Model of the Tully-Fisher and M-Sigma Relations
Authors: Gorkavyi Nick
Published: May 2022

The observed Tully-Fisher and Faber-Jackson laws between the baryonic mass of galaxies and the velocity of motion of stars at the edge of galaxies are explained within the framework of the model of accretion of galaxies around supermassive black holes (SMBH). The accretion model can also explain the M-sigma relation between the mass of a supermassive black hole and the velocity of stars in the bulge. The difference in the mechanisms of origin of elliptical galaxies with low angular momentum and disk galaxies with high angular momentum can be associated with 3D and 2D accretion.
RNA m6A Modification Changes in Postmortem Nucleus Accumbens of Subjects with Alcohol Use Disorder: A Pilot Study
Authors: Liu Ying , Zhang Huiping
Published: May 2022

Background: The nucleus accumbens (NAc) is a key brain structure mediating the rewarding effect of alcohol and drug abuse. Chronic alcohol consumption may alter RNA methylome (or epitranscriptome) in the NAc, leading to altered gene expression and thus behavioral neuroadaptation to alcohol. Methods: This pilot study profiled the epitranscriptomes of mRNAs, long noncoding RNAs (lncRNAs), and microRNAs (miRNAs) in postmortem NAc of three male Caucasian subjects with alcohol use disorder (AUD) and three matched male Caucasian control subjects using Arraystar’s m6A-mRNA&lncRNA Epitranscriptomic Microarray assay. Differentially methylated (DM) RNAs and the function of DM RNAs were analyzed by biostatistics and bioinformatics programs. Results: 26 mRNAs were hypermethylated and three mRNAs were hypomethylated in the NAc of AUD subjects (≥2-fold changes and p ≤ 0.05). Most of these 29 DM mRNAs are involved in immune-related pathways (e.g., IL-17 signaling). Moreover, four lncRNAs were hypermethylated and one lncRNA was hypomethylated in the NAc of AUD subjects (≥2-fold changes and p ≤ 0.05). Additionally, three miRNAs were hypermethylated in the NAc of AUD subjects (≥2-fold changes and p ≤ 0.05). Conclusions: This study revealed RNA methylomic changes in the NAc of AUD subjects, suggesting that chronic alcohol consumption may lead to AUD through epitranscriptomic RNA modifications. Our findings need to be replicated in a larger sample.
Characterizing Hydrological Functioning of Three Large Karst Springs in the Salem Plateau, Missouri, USA
Authors: Sarker Shishir K. , Fryar Alan E.
Published: May 2022

Spring discharge hydrographs can provide information on karst aquifer connectivity and responses to precipitation. However, few studies have conducted time-series analyses of spring hydrographs over multi-decadal time scales. We examine daily discharge for three large karst springs and daily precipitation for adjoining weather stations during 1928–2019 in the Salem Plateau of southern Missouri, one of the major karst regions in the USA. For different time periods, we conducted baseflow index calculations and time-series (autocorrelation, spectral density, and cross-correlation with precipitation) analyses for discharge data, and Mann–Kendall (MK) trend analyses for discharge and precipitation data. Hydrograph separation indicates discharge is baseflow-dominated (86–94%) at all three springs. The memory effect is lower for Bennett Spring (with an auto-correlation lag time 29–41 days) than for Big Spring (60–92 days) and Greer Spring (77–112 days). Spectral density analysis indicates that annual signals dominate all three springs. Cross-correlation analysis shows a quicker response to precipitation at Bennett Spring (0–1 days) than at Big and Greer springs (1–2 days). MK trend analysis shows significant increases in discharge for all three springs over multiple decades, but not for the period 2007–2019. Increased discharge accompanies regional increases in precipitation, but may also reflect increased recharge associated with reversion of farmland to forest.
G-Protein-Coupled Estrogen Receptor Expression in Rat Uterine Artery Is Increased by Pregnancy and Induces Dilation in a Ca2+ and ERK1/2 Dependent Manner
Authors: Tropea Teresa , Rigiracciolo Damiano , Esposito Milena , Maggiolini Marcello , Mandalà Maurizio
Published: May 2022

Increasing levels of estrogens across gestation are partly responsible for the physiological adaptations of the maternal vasculature to pregnancy. The G protein-coupled estrogen receptor (GPER) mediates acute vasorelaxing effects in the uterine vasculature, which may contribute to the regulation of uteroplacental blood flow. The aim of this study was to investigate whether GPER expression and vasorelaxation may occur following pregnancy. Elucidation of the functional signalling involved was also investigated. Radial uterine and third-order mesenteric arteries were isolated from non-pregnant (NP) and pregnant rats (P). GPER mRNA levels were determined and—concentration–response curve to the GPER-specific agonist, G1 (10−10–10−6 M), was assessed in arteries pre-constricted with phenylephrine. In uterine arteries, GPER mRNA expression was significantly increased and vasorelaxation to G1 was significantly enhanced in P compared with NP rats. Meanwhile, in mesenteric arteries, there was a similar order of magnitude in NP and P rats. Inhibition of L-type calcium channels and extracellular signal-regulated kinases 1/2 significantly reduced vasorelaxation triggered by G1 in uterine arteries. Increased GPER expression and GPER-mediated vasorelaxation are associated with the advancement of gestation in uterine arteries. The modulation of GPER is exclusive to uterine arteries, thus suggesting a physiological contribution of GPER toward the regulation of uteroplacental blood flow during pregnancy.
PIF4 Promotes Expression of HSFA2 to Enhance Basal Thermotolerance in Arabidopsis
Authors: Yang Jiaheng , Qu Xiao , Ji Li , Li Guanhui , Wang Chen , Wang Changyu , Zhang Yan , Zheng Lanjie , Li Wanchen , Zheng Xu
Published: May 2022

Heat stress (HS) seriously restricts the growth and development of plants. When plants are exposed to extreme high temperature, the heat stress response (HSR) is activated to enable plants to survive. Sessile plants have evolved multiple strategies to sense and cope with HS. Previous studies have established that PHYTOCHROME INTERACTING FACTOR 4 (PIF4) acts as a key component in thermomorphogenesis; however, whether PIF4 regulates plant thermotolerance and the molecular mechanism linking this light transcriptional factor and HSR remain unclear. Here, we show that the overexpression of PIF4 indeed provides plants with a stronger basal thermotolerance and greatly improves the survival ability of Arabidopsis under severe HS. Via phylogenetic analysis, we identified two sets (six) of PIF4 homologs in wheat, and the expression patterns of the PIF4 homologs were conservatively induced by heat treatment in both wheat and Arabidopsis. Furthermore, the PIF4 protein was accumulated under heat stress and had an identical expression level. Additionally, we found that the core regulator of HSR, HEAT SHOCK TRANSCRIPTION FACTOR A2 (HSFA2), was highly responsive to light and heat. Followed by promoter analysis and ChIP-qPCR, we further found that PIF4 can bind directly to the G-box motifs of the HSFA2 promoter. Via effector–reporter assays, we found that PIF4 binding could activate HSFA2 gene expression, thereby resulting in the activation of other HS-inducible genes, such as heat shock proteins. Finally, the overexpression of PIF4 led to a stronger basal thermotolerance under non-heat-treatment conditions, thereby resulting in an enhanced tolerance to severe heat stress. Taken together, our findings propose that PIF4 is linked to heat stress signaling by directly binding to the HSFA2 promoter and triggering the HSR at normal temperature conditions to promote the basal thermotolerance. These functions of PIF4 provide a candidate direction for breeding heat-resistant crop cultivars.
Podocyte-Related Mechanisms Underlying Survival Benefit of Long-Term Angiotensin Receptor Blocker
Authors: Zhu Xuejing , Gao Dan , Albertazzi Vittorio , Zhong Jianyong , Ma Li-Jun , Du Liping , Shyr Yu , Kon Valentina , Yang Hai-Chun , Fogo Agnes B.
Published: May 2022

We previously found that short-term treatment (week 8 to 12 after injury) with high-dose angiotensin receptor blocker (ARB) induced the regression of existing glomerulosclerosis in 5/6 nephrectomy rats. We therefore assessed the effects of long-term intervention with ARB vs. nonspecific antihypertensives in this study. Adult rats underwent 5/6 nephrectomy and renal biopsy 8 weeks later. The rats were then divided into three groups with equivalent renal function and glomerular sclerosis and treated with high-dose losartan (ARB), nonspecific antihypertensive triple-therapy (TRX), or left untreated (Control) until week 30. We found that blood pressure, serum creatinine levels, and glomerulosclerosis were lower at sacrifice in ARB and TRX vs. Control. Only ARB reduced proteinuria and maintained the density of WT-1-positive podocytes. Glomerular tufts showed more double-positive cells for CD44, a marker of activated parietal epithelial cells, and synaptopodin after ARB vs. TRX or Control. ARB treatment reduced aldosterone levels. ARB-treated rats had significantly improved survival when compared with TRX or Control. We conclude that both long-term ARB and triple-therapy ameliorate progression, but do not sustain the regression of glomerulosclerosis. ARB resulted in the superior preservation of podocyte integrity and decreased proteinuria and aldosterone, linked to increased survival in the uremic environment.
Silver Nanoparticles Targeting the Drug Resistance Problem of Streptococcus dysgalactiae: Susceptibility to Antibiotics and Efflux Effect
Authors: Garibo Ruiz Diana , Nefedova Ekaterina , Shkil Nikolay N. , Shkil Nikolay A. , Vazquez-Gomez Roberto Luna , Pestryakov Alexey , Bogdanchikova Nina
Published: May 2022

The present work is a continuation of our translational research focusing on the use of silver nanoparticles (AgNPs) to solve the global problem of antibiotic resistance. In vivo fieldwork was done with 300 breeding farm cows with serous mastitis. Ex vivo assays revealed that after cow treatment with the antibiotic drug Spectromast LCTM, S.dysgalactiae susceptibility to 31 antibiotics dropped by 22.9%, but after treatment with Argovit–CTM AgNPs, it was raised by 13.1%. This was explained by the fact that the percentage of isolates with an efflux effect after Spectromast LC treatment resulted in an 8% increase, while Argovit-C-treatment caused a 19% decrease. The similarity of these results to our previous results on S. aureus isolates from mastitis cows treated with the antibiotic drug Lactobay and Argovit–CTM AgNPs was shown. So, mastitis treatments with Argovit-CTM AgNPs can partially return the activity of antibiotics towards S.dysgalactiae and S. aureus, while, in contrast, treatments with antibiotic drugs such as Spectromast LC and Lactobay enhance bacterial resistance to antibiotics. The results of this work strengthen the hope that in the future the use of AgNPs as efflux pump inhibitors will recover the activity of antibiotics, and thus will preserve the wide spectrum of antibiotics on the market.
Optimization of Aedes albopictus (Diptera: Culicidae) Mass Rearing through Cost-Effective Larval Feeding
Authors: Kavran Mihaela , Puggioli Arianna , Šiljegović Sara , Čanadžić Dušan , Laćarac Nikola , Rakita Mina , Ignjatović Ćupina Aleksandra , Balestrino Fabrizio , Petrić Dušan , Bellini Romeo
Published: May 2022

Aedes (Stegomyia) albopictus (Skuse, 1895) is an invasive important medical and veterinary pest species. The sterile insect technique (SIT) involves the mass rearing of males, and their sterilization and release into the habitat to compete with wild males. Our research objective was to compare the effectiveness of three larval diet recipes (IAEA-BY, BCWPRL, and MIX-14) in the laboratory rearing of Ae. albopictus males to evaluate the available economical feeding alternatives. The separation of sexes was done in the pupal stage by sieving. Reared males were tested for flight capacity and longevity. The application of the BCWPRL diet resulted in a higher portion of sieved male pupae than females, but the development of males was the slowest, and the number of obtained males (pupae and adults) was lower compared to the other two diets. The adult mean survival time was the highest in males fed with MIX-14 and the lowest in males fed with IAEA-BY. Males fed by IAEA-BY also demonstrated higher initial mortality in the adult stage. The diets BCWPRL and MIX-14 are economically more convenient than IAEA-BY (2.28 and 5.30 times cheaper, respectively). The cheapest diet, MIX-14, might represent a candidate for replacing the effective but still expensive IAEA-BY larval diet, providing lower costs of sterile male production.
Geostatistical Inversion of Spectrally Broadened Seismic Data for Re-Evaluation of Oil Reservoir Continuity in Inas Field, Offshore Malay Basin
Authors: Nwafor Basil Onyekayahweh , Hermana Maman , Elsaadany Mohamed
Published: May 2022

The application of geostatistics in seismic inversion techniques has been proven somewhat reliable in the delineation of reservoir properties and has recently attracted the attention of many geoscientists. However, there are cases where its prediction returned negative results after drilling. In this research, we re-evaluated a reservoir in Inas Field, whose geostatistical inversion result wrongly predicted sand continuity, resulting in the spudding of a dry hole. When a geostatistical seismic inversion is successfully applied, it provides an increase in seismic resolution and aids the prediction of sand continuity. Although this method relies more on the statistical data from a well because of the limitation of the seismic data in resolving thin geologic features, the spatial variation of reservoir parameters still depends on seismic data, which often have poor resolution quality. Therefore, to investigate the impact of bandlimited data on the geostatistical inversion, we harmonically extended the seismic bandwidth by applying a sparse-layer spectral inversion algorithm to the data. This algorithm increased the seismic data bandwidth from 80 Hz to 180 Hz, and its tuning thickness reduced from 32 m to 10 m at the reservoir interval. The resultant broadband (180 Hz), as well as the original seismic (narrowband of 80 Hz) data, were both used as input to build two separate geostatistical prediction models, respectively. Twenty (20) realizations of these models were generated, ranked into P10, P50, and P90, and the best case was selected for interpretation. These realizations were used to characterize the reservoir lithofacies distribution. When compared, the result of the broadband inversion, facies and sand distribution model showed that the reservoir facies changed towards the location of the dry well. The broadband geostatistical inversion efficiently improved the reservoir characterization process by not only producing an accurate estimation of the lateral extent of the reservoir heterogeneities but also generating outcomes that help us understand why other geostatistical inversion analyses of the target reservoir were misleading. Contrary to the popular assumption, it was discovered that the tuning effects of bandlimited data could affect the result of a geostatistical inversion and result in wrong facies predictions.
Model Test of the Pullout Bearing Capacity of End-Bearing Torpedo Anchors
Authors: Li Gang , Zhang Jinli , Liu Jia
Published: May 2022

Torpedo anchors (TAs) are regarded as one of the most efficient mooring solutions for taut mooring systems, and end-bearing TAs are a new type of TA that primarily relies on end-bearing plates at the tail to generate a pile-end resistance to improve their pulloutbearing capacity (P). Therefore, the estimation of the pullout capacity of the end-bearing TA is vital for the design of offshore floating facilities. In this study, pullout model tests were conducted to investigate the P of conventional and end-bearing TAs and examine the effects of factors such as the embedment depth (h), the relative density (Dr), the pullout angle (α), and the area (A) of the bearing plates on P. The test results show that, under oblique pullout loading, the P on the conventional TA increased slowly as displacement increased, while there was a peak on the load–displacement curve of each end-bearing TA with a relatively large A. The end-bearing TAs considerably outperformed the conventional TAs in terms of the P. In addition, increasing h, Dr, and A significantly increased the P of the end-bearing TAs. However, increasing h and Dr slightly decreased the ability of the bearing plates to increase the P of the end-bearing TAs. These research results can provide a guideline for TA installation in deep-sea engineering.
Revealing the Effectiveness of Fisheries Policy: A Biological Observation of Species Johnius belengerii in Xiamen Bay
Authors: Huang Liang-Min , Wang Jia-Qiao , Shih Yi-Jia , Li Jun , Chu Ta-Jen
Published: May 2022

The rapid development of China’s economy has brought tremendous pressure to the marine ecosystem, and about 57% of marine fish populations have been overexploited or collapsed. A series of fisheries policies have been implemented successively to improve the decline of resources. Over the past decade, the fisheries sector has particularly increased focus on resource and ecosystem sustainability, which has led to the wider use of stock management policies in China. Therefore, fishery resource assessment is crucial, such as assessing the long-term changes in biological information. This study is based on biological characteristics of Johnius belengerii captured by bottom trawls in Xiamen Bay during two periods, beginning in 2006 and 2016. Length composition, length–weight relationship, growth, mortality, sexual maturation, and feeding intensity were analyzed. The changes in biological characteristics show that there is a phenomenon of improvement in the later period, of which the changes are closely related to a series of fisheries management strategies, such as setting closed fishing periods and non-fishing areas, and establishing fishery restoration marine protected areas. This result seems to reveal the effectiveness of a long-term series of fisheries policies. It can provide an important basis and visibility for management effectiveness.
Torulaspora delbrueckii Phenotypic and Metabolic Profiling towards Its Biotechnological Exploitation
Authors: Silva-Sousa Flávia , Fernandes Ticiana , Pereira Fábio , Rodrigues Diana , Rito Teresa , Camarasa Carole , Franco-Duarte Ricardo , Sousa Maria João
Published: May 2022

Wine is a particularly complex beverage resulting from the combination of several factors, with yeasts being highlighted due to their fundamental role in its development. For many years, non-Saccharomyces yeasts were believed to be sources of spoilage and contamination, but this idea was challenged, and many of these yeasts are starting to be explored for their beneficial input to wine character. Among this group, Torulaspora delbrueckii is gaining relevance within the wine industry, owing to its low volatile acidity production, increased release of aromatic compounds and enhanced color intensity. In addition, this yeast was also attracting interest in other biotechnological areas, such as bread and beer fermentation. In this work, a set of 40 T. delbrueckii strains, of varied geographical and technological origins, was gathered in order to characterize the phenotypic behavior of this species, focusing on different parameters of biotechnological interest. The fermentative performance of the strains was also evaluated through individual fermentations in synthetic grape must with the isolates’ metabolic profile being assessed by HPLC. Data analysis revealed that T. delbrueckii growth is significantly affected by high temperature (37 °C) and ethanol concentrations (up to 18%), alongside 1.5 mM SO2, showing variable fermentative power and yields. Our computation models suggest that the technological origin of the strains seems to prevail over the geographical origin as regards the influence on yeast properties. The inter-strain variability and profile of the products through the fermentative processes reinforce the potential of T. delbrueckii from a biotechnological point of view.
Ethylene Promotes Expression of the Appressorium- and Pathogenicity-Related Genes via GPCR- and MAPK-Dependent Manners in Colletotrichum gloeosporioides
Authors: Ren Dandan , Wang Tan , Zhou Ganghan , Ren Weiheng , Duan Xiaomin , Gao Lin , Chen Jiaxu , Xu Ling , Zhu Pinkuan
Published: May 2022

Ethylene (ET) represents a signal that can be sensed by plant pathogenic fungi to accelerate their spore germination and subsequent infection. However, the molecular mechanisms of responses to ET in fungi remain largely unclear. In this study, Colletotrichum gloeosporioides was investigated via transcriptomic analysis to reveal the genes that account for the ET-regulated fungal development and virulence. The results showed that ET promoted genes encoding for fungal melanin biosynthesis enzymes, extracellular hydrolases, and appressorium-associated structure proteins at 4 h after treatment. When the germination lasted until 24 h, ET induced multiple appressoria from every single spore, but downregulated most of the genes. Loss of selected ET responsive genes encoding for scytalone dehydratase (CgSCD1) and cerato-platanin virulence protein (CgCP1) were unable to alter ET sensitivity of C. gloeosporioides in vitro but attenuated the influence of ET on pathogenicity. Knockout of the G-protein-coupled receptors CgGPCR3-1/2 and the MAPK signaling pathway components CgMK1 and CgSte11 resulted in reduced ET sensitivity. Taken together, this study in C. gloeosporioides reports that ET can cause transcription changes in a large set of genes, which are mainly responsible for appressorium development and virulence expression, and these processes are dependent on the GPCR and MAPK pathways.
Fungal–Bacterial Networks in the Habitat of SongRong (Tricholoma matsutake) and Driving Factors of Their Distribution Rules
Authors: Ji Rui-Qing , Xu Yang , Si Yan-Ji , Phukhamsakda Chayanard , Li Yu , Meng Li-Peng , Liu Shu-Yan , Xie Meng-Le
Published: May 2022

Soil origin, mycorrhizal plant partners and environmental factors affect the growth and development of SongRong (Tricholoma matsutake). In order to clarify the relationships of fungi–bacteria networks and various influence factors in the habitat of SongRong, we chose three collection sites with a Quercus mongolica pure forest (plot A without SongRong was used as the control sample site), Q. mongolica mixed Rhododendron dauricum (plot B) and Q. mongolica mixed with R. dauricum and Pinus densiflora (plot C). By using high-throughput sequencing, we obtained a total of 4930 fungal and 55501 bacterial amplicon sequence variants (ASVs) based on internally transcribed spacer ribosomal RNA (ITS rRNA) and 16S ribosomal RNA (16S rRNA) sequencing via the Illumina NovaSeq platform. In the habitat soil of SongRong (plot B and plot C), alpha or beta diversity and species compositions of fungi and bacteria were different from plot A. The fungal–bacterial networks follow the selection rule that few dominant genera account for the greater relative abundance. Forest types, but not the host itself, drove the fungal–bacterial networks of the forest soil, and soil physicochemical characteristics and texture affected their abundance. The abundance of Tricholoma was affected by the fungal and bacterial abundance in the habitat.
Diagnostic Challenge of Invasive Lobular Carcinoma of the Breast: What Is the News? Breast Magnetic Resonance Imaging and Emerging Role of Contrast-Enhanced Spectral Mammography
Authors: Costantini Melania , Montella Rino Aldo , Fadda Maria Paola , Tondolo Vincenzo , Franceschini Gianluca , Bove Sonia , Garganese Giorgia , Rinaldi Pierluigi Maria
Published: May 2022

Invasive lobular carcinoma is the second most common histologic form of breast cancer, representing 5% to 15% of all invasive breast cancers. Due to an insidious proliferative pattern, invasive lobular carcinoma remains clinically and radiologically elusive in many cases. Breast magnetic resonance imaging (MR) is considered the most accurate imaging modality in detecting and staging invasive lobular carcinoma and it is strongly recommended in pre-operative planning for all ILC. Contrast-enhanced spectral mammography (CESM) is a new diagnostic method that enables the accurate detection of malignant breast lesions similar to that of breast MR. CESM is also a promising breast imaging method for planning surgeries. In this study, we compare the ability of contrast-enhanced spectral mammography (CESM) with breast MR in the preoperative assessment of the extent of invasive lobular carcinoma. All patients with proven invasive lobular carcinoma treated in our breast cancer center underwent preoperative breast MRI and CESM. Images were reviewed by two dedicated breast radiologists and results were compared to the reference standard histopathology. CESM was similar and in some cases more accurate than breast MR in assessing the extent of disease in invasive lobular cancers. Further evaluation in larger prospective randomized trials is needed to validate our preliminary results.
Sex-Based Difference in the Effect of Metoprolol on Heart Rate and Bradycardia in a Population-Based Setting
Authors: Hendriksen Linda C. , Omes-Smit Grace , Koch Birgit C. P. , Ikram M. Arfan , Stricker Bruno H. , Visser Loes E.
Published: May 2022

Background: Metoprolol, a beta-blocker, is used to reduce the heart rate. Although it has been demonstrated that the metoprolol plasma concentration is higher in women than in men, the same dose is recommended. In this study, we investigated whether the metoprolol concentration was associated with a stronger heart-rate reduction and bradycardia in women than in men. Methods: This study is part of the Rotterdam Study (RS), a population-based prospective cohort study. Blood samples from a random subset of 2000 participants were used to assess metoprolol plasma levels. An analysis of heart rate (beats per minute, bpm) and bradycardia (<60 bpm) was performed in metoprolol users with an ECG at the day of blood collection to study sex-specific differences in heart rate and the risk of bradycardia. Results: In total, 40 women and 39 men were included. There was a statistically significant association between metoprolol concentration and heart rate in women (p-value: 0.014) but not in men (p-value: 0.639). Furthermore, women in the highest concentration group had a more than 15-times-higher risk of bradycardia than women in the lowest concentration group (OR = 15.6; 95% CI = 1.1, 217.3); however, this was not seen in men (OR = 1.3; 95% CI = 0.1, 12.4). After adjustment for age, BMI, time between blood sample and ECG, hypertension, myocardial infarction, heart failure, atrial fibrillation, digoxin use, and calcium channel blocker use, the association between concentration and bradycardia in women remained statistically significant. Conclusions: Women, but not men, had a statistically significantly lower heart rate at higher metoprolol plasma concentration and a statistically significantly increased risk of bradycardia.
Comparison of Surgical Outcomes of Hysterectomy by Vaginal Natural Orifice Transluminal Endoscopic Surgery (vNOTES) versus Single-Port Access (SPA) Surgery
Authors: Noh Joseph J. , Kim Myeong-Seon , Kang Jun-Hyeok , Jung Ji-Hee , Chang Chi-Son , Jeon Jungeun , Kim Tae-Joong
Published: May 2022

Single-port access (SPA) laparoscopic surgery and vaginal natural orifice transluminal endoscopic surgery (vNOTES) have many advantages. The objective of the present study is to compare patient characteristics, operative details, and postoperative outcomes between the two surgical methods. Patients who were planned to undergo vNOTES or SPA laparoscopic surgery between April 2020 and June 2021 were prospectively enrolled. The surgical method was determined by a single surgeon after imaging results evaluation and a physical exam. Those who had favorable pelvic conditions without any evidence of adhesion were scheduled for vNOTES. A total of 33 patients underwent a vNOTES hysterectomy while 40 patients received a SPA laparoscopic hysterectomy. All surgeries were performed by one surgeon. The proportion of the patients who had a history of vaginal delivery was significantly higher in the vNOTES group. The operative time for port installation was significantly longer in the vNOTES group, but the total operative time was shorter compared to the SPA group. The postoperative pain scores 12 h after the operations were also significantly lower in the vNOTES group. Other surgical outcomes were comparable between the two groups. The present study demonstrated that the early operative outcomes of vNOTES hysterectomy were comparable to those of SPA hysterectomy. It also highlights the importance of adequate patient selection when determining surgical methods based on imaging results and physical examinations.
Antibiotic Prescription and In-Hospital Mortality in COVID-19: A Prospective Multicentre Cohort Study
Authors: Pinte Larisa , Ceasovschih Alexandr , Niculae Cristian-Mihail , Stoichitoiu Laura Elena , Ionescu Razvan Adrian , Balea Marius Ioan , Cernat Roxana Carmen , Vlad Nicoleta , Padureanu Vlad , Purcarea Adrian , Badea Camelia , Hristea Adriana , Sorodoc Laurenţiu , Baicus Cristian
Published: May 2022

Background: Since the beginning of the COVID-19 pandemic, empiric antibiotics (ATBs) have been prescribed on a large scale in both in- and outpatients. We aimed to assess the impact of antibiotic treatment on the outcomes of hospitalised patients with moderate and severe coronavirus disease 2019 (COVID-19). Methods: We conducted a prospective multicentre cohort study in six clinical hospitals, between January 2021 and May 2021. Results: We included 553 hospitalised COVID-19 patients, of whom 58% (311/553) were prescribed antibiotics, while bacteriological tests were performed in 57% (178/311) of them. Death was the outcome in 48 patients—39 from the ATBs group and 9 from the non-ATBs group. The patients who received antibiotics during hospitalisation had a higher mortality (RR = 3.37, CI 95%: 1.7–6.8), and this association was stronger in the subgroup of patients without reasons for antimicrobial treatment (RR = 6.1, CI 95%: 1.9–19.1), while in the subgroup with reasons for antimicrobial therapy the association was not statistically significant (OR = 2.33, CI 95%: 0.76–7.17). After adjusting for the confounders, receiving antibiotics remained associated with a higher mortality only in the subgroup of patients without criteria for antibiotic prescription (OR = 10.3, CI 95%: 2–52). Conclusions: In our study, antibiotic treatment did not decrease the risk of death in the patients with mild and severe COVID-19, but was associated with a higher risk of death in the subgroup of patients without reasons for it.