bluuebunny/crossref_metadata_2025 huggingface dataset:
Cation/Proton Antiporter Genes in Tomato: Genomic Characterization, Expression Profiling, and Co-Localization with Salt Stress-Related QTLs
Authors: Hussain Zahid , Khan Habiba , Imran Muhammad , Naeem Muhmmad Kashif , Shah Sabir Hussain , Iqbal Arshad , Ali Syed Shujait , Rizwan Muhammad , Ali Shahid , Muneer Muhammad Atif , Widemann Emilie , Shafiq Sarfraz
Published: January 2022

The cation/proton antiporter (CPA) family represents a class of transmembrane transporter proteins that play a crucial role in plants during high salinity stress by maintaining the cell’s ionic balance and pH homeostasis. So far, the CPA genes have not been systematically characterized in tomato (Solanum lycopersicum). In this study, we identified and analyzed 33 putative CPA genes in tomato. Phylogenetic analysis showed that tomato CPAs could be classified into three subgroups, i.e., CHX (18 genes), KEA (8 genes), and NHX (7 genes). CPA genes within each subgroup shared similar motifs, conserved catalytic domains and gene structure. Further analysis revealed that the CPA genes were unevenly distributed on the chromosomes and segmental duplication events played a major role in the expansion of the CPA gene family in tomato. Gene expression analysis exhibited that CPA genes were differentially expressed in different tissues, various stages of fruit development, and differentially regulated in response to abiotic stresses, especially salt stress. Further, co-localization of tomato CPA genes with quantitative trait loci (QTL) of salt stress-related phenotypes revealed their broader functions in salt stress tolerance. Finally, predicted protein–protein interactions of tomato CPAs, gene ontology analysis, and the presence of putative cis-elements in the promoter further support the diverse role of tomato CPAs in plant development and plant stress tolerance. In brief, this study highlights the potential role of tomato CPAs in plant development and abiotic stress tolerance, especially in salt stress, and provides comprehensive information to explore new candidate genes for salt tolerance in tomato.
Reduction of the Economic Risk by Adaptation Measures to Alleviate Heat Stress in Confined Buildings for Growing-Fattening Pigs Modelled by a Projection for Central Europe in 2030
Authors: Schauberger Günther , Schönhart Martin , Zollitsch Werner , Hörtenhuber Stefan J. , Kirner Leopold , Mikovits Christian , Baumgartner Johannes , Piringer Martin , Knauder Werner , Anders Ivonne , Andre Konrad , Hennig-Pauka Isabel
Published: January 2022

Economic risks for livestock production are caused by volatile commodities and market conditions, but also by environmental drivers such as increasing uncertainties due to weather anomalies and global warming. These risks impact the gross margin of farmers and can stimulate investment decisions. For confined pig and poultry production, farmers can reduce the environmental impact by implementing specific adaptation measures (AMs) to reduce heat stress. A simulation model driven by meteorological data was used to calculate indoor climate for 1981 to 2017. This dataset was extrapolated for a projection in the year 2030. The heat stress was calculated for a business-as-usual livestock building and for several AMs. For 2030, the expected value of the reduction of the gross margin was calculated by EUR 3.98 a−1 per animal place for the business-as-usual scenario. The results show that only energy-saving adaptation measures to reduce the inlet air temperature are appropriate to reduce the economic risk to the level of the year 1980 between EUR 0.03 a−1 and EUR 1.02 a−1 per animal place. The efficiency of other AMs to reduce heat stress is distinctly lower (between EUR 2.62 a−1 and EUR 3.80 a−1 per animal place). The results in this study can support the decision making of farmers concerning adaptation management and investments. It can inform agricultural policy design as well as technological development.
Evaluation of a Precision Biotic on the Growth Performance, Welfare Indicators, Ammonia Output, and Litter Quality of Broiler Chickens
Authors: Jacquier Vincent , Walsh Maria Carol , Schyns Ghislain , Claypool Joshua , Blokker Britt , Bortoluzzi Cristiano , Geremia Jack
Published: January 2022

A dietary glycan-based precision biotic (Glycan PB) was evaluated on the performance, welfare indicators, and litter characteristics of broiler chickens. In Trial 1, the main effects of Glycan PB dose (0, 250 and 500 g/metric ton (MT)) and xylanase supplementation (0 or 100 g/MT) were tested, as was their interaction. In Trial 2, pens located inside a commercial house were used to test the effect of Glycan PB supplementation (500 g/MT) versus a control diet. In Trial 1, Glycan PB supplementation at 250 and 500 g/MT improved feed conversion ratio (FCR) by 7 and 11 points when compared to diets without Glycan PB (p < 0.001). At 35 d, Glycan PB reduced the pH and ammonia concentration in diets with xylanase. In Trial 1, the supplementation with 500 g of Glycan PB/MT of feed reduced litter scores (p < 0.05). In both trials, 500 g of Glycan PB/MT of feed increased the proportions of birds without footpad lesions (Trial 1: 72.2% vs. 82.7%; p < 0.001; Trial 2: 14 to 27.3% (p = 0.05) or gait defects (Trial 1: 96.1% vs. 98.4%; p < 0.001) and decreased the proportion of birds with footpad lesions (Trial 2: 86% vs. 72.7%; p = 0.05).
The Implications of Policies on the Welfare of Free-Roaming Cats in New Zealand
Authors: Sumner Christine L. , Walker Jessica K. , Dale Arnja R.
Published: January 2022

A lack of national legislation for cat management in New Zealand poses challenges for ensuring that practices are consistently humane and effective. In this paper, we review the current cat management policies in New Zealand and the implications they have on the welfare of free-roaming cats (from here on, referred to as ‘cats’). Our review demonstrates that there are multiple policy mechanisms used to manage cats in New Zealand for a variety of reasons, including animal welfare, pest management, and nuisance, and that these different policies have both positive and negative implications for cat welfare. We provide context pertaining to New Zealanders’ acceptance of current or future laws and regulations and compare the New Zealand policy landscape with other countries, with a particular emphasis on Australia, to identify potential directions and outcomes of increased regulation. We discuss the future of the regulatory environment in New Zealand, including the need to better understand the impact of policies on cats, people, and other animals in urban, rural, and wild spaces. We further discuss the need to better understand the cat–human relationship for future policy decisions and offer a solution based on national cat legislation.
Effects of Dietary Supplementation with Red Yeast (Sporidiobolus pararoseus) on Productive Performance, Egg Quality, and Duodenal Cell Proliferation of Laying Hens
Authors: Kanmanee Chanidapha , Srinual Orranee , Punyatong Montri , Moonmanee Tossapol , Lumsangkul Chompunut , Tangtaweewipat Suchon , Van Doan Hien , Yachai Mongkol , Chaiyaso Thanongsak , Tapingkae Wanaporn
Published: January 2022

Nowadays, industrial poultry producers are more focused on the safety of their products, especially contaminants from feedstuffs such as mycotoxin and pesticides. The residue from animal production using antibiotic growth promoters (AGPs) may cause some problems with antimicrobial resistance in human and animals. Red yeast (Sporidiobolus pararoseus) has a cell wall consisting of β-glucan and mannan-oligosaccharides and pigments from carotenoids that may be suitable for use as a substitute for AGPs. The objective was to evaluate the effects of red yeast in laying hen diets on productive performance, egg quality, and duodenal health. A total of 22-week-old laying hens (n = 480) were divided into five groups: control diet (CON), AGP at 4.5 g/kg and red yeast supplementation at 1.0 (RY1.0), 2.0 (RY2.0) and 4.0 g/kg (RY4.0) of diet. The results show that the AGP, RY2.0, and RY4.0 groups had significantly higher final body weight compared with the other groups (p < 0.001). The red yeast supplementation improved the egg shape index (p = 0.025), Haugh unit (p < 0.001), and yolk color (p = 0.037), and decreased yolk cholesterol (p < 0.001). Diet with red yeast supplementation improved villus height to crypt depth ratio and crypt cell proliferations. In conclusion, red yeast supplementation at 2.0 g/kg of diet can substitute AGP in layer diet.
Oxidative Stress in the Pathogenesis of Aorta Diseases as a Source of Potential Biomarkers and Therapeutic Targets, with a Particular Focus on Ascending Aorta Aneurysms
Authors: Pisano Calogera , Benedetto Umberto , Ruvolo Giovanni , Balistreri Carmela Rita
Published: January 2022

Aorta diseases, such as ascending aorta aneurysm (AsAA), are complex pathologies, currently defined as inflammatory diseases with a strong genetic susceptibility. They are difficult to manage, being insidious and silent pathologies whose diagnosis is based only on imaging data. No diagnostic and prognostic biomarkers or markers of outcome have been known until now. Thus, their identification is imperative. Certainly, a deep understanding of the mechanisms and pathways involved in their pathogenesis might help in such research. Recently, the key role of oxidative stress (OS) on the pathophysiology of aorta disease has emerged. Here, we describe and discuss these aspects by revealing some OS pathways as potential biomarkers, their underlying limitations, and potential solutions and approaches, as well as some potential treatments.
COVID-19 during Gestation: Maternal Implications of Evoked Oxidative Stress and Iron Metabolism Impairment
Authors: Moreno-Fernandez Jorge , Ochoa Julio J. , De Paco Matallana Catalina , Caño Africa , Martín-Alvarez Estefania , Sanchez-Romero Javier , Toledano Juan M. , Puche-Juarez Maria , Prados Sonia , Ruiz-Duran Susana , Diaz-Meca Lucia , Carrillo María Paz , Diaz-Castro Javier
Published: January 2022

COVID-19 has reached pandemic proportions worldwide, with considerable consequences for both health and the economy. In pregnant women, COVID-19 can alter the metabolic environment, iron metabolism, and oxygen supply of trophoblastic cells, and therefore have a negative influence on essential mechanisms of fetal development. The purpose of this study was to investigate, for the first time, the effects of COVID-19 infection during pregnancy with regard to the oxidative/antioxidant status in mothers’ serum and placenta, together with placental iron metabolism. Results showed no differences in superoxide dismutase activity and placental antioxidant capacity. However, antioxidant capacity decreased in the serum of infected mothers. Catalase activity decreased in the COVID-19 group, while an increase in 8-hydroxy-2’-deoxyguanosine, hydroperoxides, 15-FT-isoprostanes, and carbonyl groups were recorded in this group. Placental vitamin D, E, and Coenzyme-Q10 also showed to be increased in the COVID-19 group. As for iron-related proteins, an up-regulation of placental DMT1, ferroportin-1, and ferritin expression was recorded in infected women. Due to the potential role of iron metabolism and oxidative stress in placental function and complications, further research is needed to explain the pathogenic mechanism of COVID-19 that may affect pregnancy, so as to assess the short-term and long-term outcomes in mothers’ and infants’ health.
Lipid Profile, Antioxidant and Antihypertensive Activity, and Computational Molecular Docking of Diatom Fatty Acids as ACE Inhibitors
Authors: Pekkoh Jeeraporn , Phinyo Kittiya , Thurakit Theera , Lomakool Sureeporn , Duangjan Kritsana , Ruangrit Khomsan , Pumas Chayakorn , Jiranusornkul Supat , Yooin Wipawadee , Cheirsilp Benjamas , Pathom-aree Wasu , Srinuanpan Sirasit
Published: January 2022

Diatoms, as single cell eukaryotic microalgae, are rich sources of lipids, which have either beneficial or detrimental effects on the prevention and treatment of many diseases. Gas chromatography-mass spectrometry (GC-MS) identified diatom lipids with high levels of essential fatty acids (EFAs), especially polyunsaturated FAs (PUFAs) containing both omega-3 and omega-6. Nutritional values of FAs indicated possible applications in the pharmaceutical, nutraceutical, and functional food industries. Diatom FAs showed antioxidative potential on harmful radicals by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging, with high inhibition of the angiotensin-converting enzyme (ACE) that causes cardiovascular disease (CVD) and hypertension. A computational molecular docking simulation confirmed the inhibition mechanisms of FAs on ACE, with comparable levels of binding free energy to chemically synthesized ACE drugs. Findings suggested that diatom lipids showed potential for use as alternative ACE inhibitors or food supplement for CVD prevention.
Prophylactic and Ameliorative Effects of PPAR-γ Agonist Pioglitazone in Improving Oxidative Stress, Germ Cell Apoptosis and Inflammation in Gentamycin-Induced Testicular Damage in Adult Male Albino Rats
Authors: El-Sayed Karima , Ali Dina A. , Maher Shymaa Ahmed , Ghareeb Dalia , Selim Samy , Albogami Sarah , Fayad Eman , Kolieb Eman
Published: January 2022

Peroxisome proliferator-activated receptor gamma (PPAR-γ) is ubiquitously expressed in testicular tissue and plays a crucial role in regulating various physiological processes. Pioglitazone (PIO) is one of the PPAR-γ agonists, having anti-oxidant and anti-inflammatory effects. Patients on gentamycin treatment may undergo serious side effects such as testicular damage. To the best of our knowledge, this was the first study to investigate the possible protective anti-inflammatory and anti-apoptotic effects of PIO on gentamycin-induced testicular damage. Fifty adult male Wistar albino rats included in the study as the control group (CTL) received normal saline; a gentamycin-induced testicular damage group (GM) received gentamycin (100 mg/kg); PIO5, PIO10, PIO20 groups received PIO at a dose of 5, 10, and 20 mg/ kg, respectively, for 21 days, and gentamycin was started at day 15 of the experiment for 6 days. The parameters of spermatozoa and histopathological alterations in the testes were significantly improved in the PIO20 group. Moreover, MDA levels, inflammatory mediators, and apoptotic Bax expression were decreased. The activity of glutathione peroxidase, catalase, total antioxidant capacity, and anti-apoptotic Bcl-2 genes expression were increased. It was concluded that PIO20 could protect against gentamycin-induced testicular damage in Wistar rats through its anti-oxidant, anti-inflammatory, and antiapoptotic effects.
Effects on Cell Growth, Lipid and Biochemical Composition of Thalassiosira weissflogii (Bacillariophyceae) Cultured under Two Nitrogen Sources
Authors: Hernández-Sandoval Francisco , Del Ángel-Rodríguez Jorge , Núñez-Vázquez Erick , Band-Schmidt Christine , Arredondo-Vega Bertha , Campa-Córdova Ángel , Moreno-Legorreta Manuel , Fernández-Herrera Leyberth , López-Cortés David
Published: January 2022

The protein and polyunsaturated fatty acid (PUFA) enrichment of microalgae can improve their nutritional value for larvae of various reared organisms. Protein enrichment can be achieved by increasing nitrogen concentration and selecting nitrogen sources that are easy to assimilate during microalga culture. Nitrogen-rich cultures can increase organism growth, biomass, and protein content, but their lipid content tends to stall. Since the diatom Thalassiosira weissflogii is usually provided to feed shrimp larvae, this study evaluated its digestibility and biochemical composition, culturing it with two nitrogen sources (NaNO3 and NH4Cl) at different concentrations (111.25, 222.50, 445 and 890 µM). The cell abundance, dry-weight biomass, Chl a, proteins, carbohydrates, total lipids and essential fatty acids were determined. The cell density and biomass peaked faster (day 12) with treatment < 890 µM than with 890 µM (day 15) in both nitrogen sources. However, the highest cell density, biomass and peak protein yield were not significantly different among treatments, suggesting the need to compare maintenance costs for a given production. After nine days of culture, concentrations ≤ 222.5 µM increased lipid content irrespective of the nitrogen source and decreased by 10–20% afterwards. With higher lipid production, the dominant PUFA were eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). One gram of NH4Cl provides ~60% more nitrogen than 1 g of NaNO3. In conclusion, based on time and growth rate, T. weissflogii cultivated with NH4Cl at 222.50 µM produced EPA and DHA at a better yield–cost ratio for biomass and lipid production. Furthermore, its nutritional value as enriched live-food for the reared larvae of marine organisms suggests potential biotechnological applications for aquaculture.
Experimental Investigation and Validation on Suppressing the Unsteady Aerodynamic Force and Flow Structure of Single Box Girder by Trailing Edge Jets
Authors: Chen Guanbin , Chen Wenli
Published: January 2022

In the present investigation, a wind tunnel experiment was performed to evaluate the effectiveness of the trailing edge jets control scheme to mitigate the unsteady aerodynamic force and flow structure of a single box girder (SBG) model. The flow control scheme uses four isolated circular holes for forming the jet flow to modify the periodic vortex shedding behind the SBG model and then alleviate the fluctuation of the aerodynamic force acting on the test model. The Reynolds number is calculated as 2.08 × 104 based on the incoming velocity and the height of the test model. A digital pressure measurement system was utilized to obtain and record the surface pressure that was distributed around the SBG model. The surface pressure results show that the fluctuating amplitude of the aerodynamic forces was attenuated in the controlled case at a specific range of the non-dimensional jet momentum coefficient. The Strouhal number of the controlled case also deviates from that of the original SBG model. Except for the pressure measurement experiment, a high-resolution digital particle image velocimetry system was applied to investigate the detailed flow structure behind the SBG model to uncover the unsteady vortex motion process from the SBG model with and without the trailing edge jets flow control. As the jet flow blows into the wake, the alternating vortex shedding mode is switched into a symmetrical shedding mode and the width of the wake flow is narrowed. The proper orthogonal decomposition was used to identify the energy of the different modes and obtain its corresponding flow structures. Moreover, the linear stability analysis of the flow field behind the SBG model shows that the scheme of trailing edge jets can dramatically suppress the area of unsteady flow.
Pumped Thermal Energy Storage System for Trigeneration: The Concept of Power to XYZ
Authors: Bellos Evangelos , Lykas Panagiotis , Tzivanidis Christos
Published: January 2022

The objective of this investigation is to present a novel concept for the optimum exploitation of volatile electricity from renewable energy sources. The idea of the Carnot battery is extended to a general concept for trigeneration which can be called “power to XYZ”. This idea is applied for the building sector where there are needs for cooling production, space-heating production/domestic hot water production and electricity. More specifically, volatile electricity feeds a multi-stage heat pump that produces cold storage at 0 °C for cooling, medium heating storage at 50 °C for space heating and high thermal storage at around 115 °C for future utilization in an organic Rankine cycle for electricity production. The storage is performed in three different temperature levels, with latent storage proposed for proper long-term and efficient storage. The use of ice is suggested especially for cold storage in order to make the design a cost-effective one. This work is a theoretical preliminary thermodynamic analysis performed with a model created in Engineering Equation Solver. The results indicate the system’s maximum exergy efficiency is found at 45.28%, while the respective energy efficiency is found at 322.16%. Moreover, this work includes parametric studies and calculations about the operating margins of the suggested system.
Targeting TGF-ß in the Central Nervous System: Assessment of Cynomolgus Monkey—Toxicity and Pharmacokinetics for an LNA-Antisense Oligonucleotide
Authors: Peters Sebastian , Wirkert Eva , Kuespert Sabrina , Heydn Rosmarie , Korte Sven , Mecklenburg Lars , Aigner Ludwig , Johannesen Siw , Bruun Tim-Henrik , Bogdahn Ulrich
Published: January 2022

Increasingly antisense oligonucleotides (ASOs) are developed for potential treatment of CNS disorders, and due to the inability to cross the blood brain barrier, they require direct administration into the cerebrospinal fluid (CSF). In this regard, intrathecal (i.th.) administration in cynomolgus monkeys (Macaca fascicularis) is a well-established approach for preclinical safety studies. Here, we present an innovative preclinical approach that is intended to support rapid entry into clinical development with ASOs targeting the CNS. The preclinical approach comprises one non-GLP study in 26 non-human primates, followed by a pivotal GLP repeated dose toxicity study in the same species. No pivotal rodent studies were conducted, and regulatory guidance to initiate this study was met by in vitro work. The non-GLP study consists of three separate phases: Phase A determines toxicity after i.th. administrations with five escalating dose levels in a single male and female animal, respectively. Dosing is conducted on days 1, 8, 15, 22, and 29 and the experiment is terminated 36 days after start of the study. The second phase (Phase B) investigates pharmacokinetics over a 2- or 4-week period at two dose levels following single administrations in eight (8) animals (4 females, 4 males). Finally, a third phase (Phase C) investigates toxicity and pharmacokinetics after repeated (9×) dosing over a 13-week period at two dose levels in sixteen (8 females, 8 males) animals. In each phase, clinical observations and physical/neurological parameters are investigated directly pre-dose, 4 h and 24 h post-dose, respectively. In all phases, CSF and blood samples are taken pre-dose and after each dosing, for determination of test article concentration, biomarkers of tolerability and biomarkers of pharmacology. In all phases, tissue samples from the liver, kidney, spinal cord, and brain are collected for determination of NVP-13 tissue concentrations. The above concept has successfully supported first-in-human clinical trials. The entire non-GLP program is completed within less than six months and requires fewer animals in comparison to the conduct of three independent studies.
A Data Transmission Method with Spectral Switches via Electroabsorption
Authors: Chang Jyun-Ping , Weng Jun-Hong , Hsu Hsun-Ching , Lee Pei-Yuan , Han Pin
Published: January 2022

In the past, the waveguide electroabsorption effect has generally been used as an intensity modulator for quasi-monochromatic light, such as lasers. Here, we study how this effect affects polychromatic light spectra. We find that for light with a Gaussian distribution spectrum, the spectral peak shift (red shift or blue shift) can be controlled by the magnitude of the applied voltage, as long as the center wavelength and the spectral band are properly selected. This result can be used as a data transmission scheme at the integrated chip level or in free space. It may offer a good option for some other light sources, such as low-cost LED or ELED (edge emitting LED), with wider spectral bandwidths.
WASN-Based Spectro-Temporal Analysis and Clustering of Road Traffic Noise in Urban and Suburban Areas
Authors: Socoró Joan Claudi , Alías Francesc , Alsina-Pagès Rosa Ma
Published: January 2022

Environmental noise has become one of the principal health risks for urban dwellers and road traffic noise (RTN) is considered to be the main source of these adverse effects. To address this problem, strategic noise maps and corresponding action plans have been developed throughout Europe in recent years in response to the European Noise Directive 2002/49/EC (END), especially in populated cities. Recently, wireless acoustic sensor networks (WASNs) have started to serve as an alternative to static noise maps to monitor urban areas by gathering environmental noise data in real time. Several studies have analysed and categorized the different acoustic environments described in the END (e.g., traffic, industrial, leisure, etc.). However, most of them have only considered the dynamic evolution of the A-weighted equivalent noise levels LAeq over different periods of time. In order to focus on the analysis of RTN solely, this paper introduces a clustering methodology to analyse and group spectro-temporal profiles of RTN collected simultaneously across an area of interest. The experiments were conducted on two acoustic databases collected during a weekday and a weekend day through WASNs deployed in the pilot areas of the LIFE+ DYNAMAP project. The results obtained show that the clustering of RTN, based on its spectro-temporal patterns, yields different solutions on weekdays and at weekends in both environments, being larger than those found in the suburban environment and lower than the number of clusters in the urban scenario.
Towards an Assembly Support System with Dynamic Bayesian Network
Authors: Precup Stefan-Alexandru , Gellert Arpad , Matei Alexandru , Gita Maria , Zamfirescu Constantin-Bala
Published: January 2022

Due to the new technological advancements and the adoption of Industry 4.0 concepts, the manufacturing industry is now, more than ever, in a continuous transformation. This work analyzes the possibility of using dynamic Bayesian networks to predict the next assembly steps within an assembly assistance training system. The goal is to develop a support system to assist the human workers in their manufacturing activities. The evaluations were performed on a dataset collected from an experiment involving students. The experimental results show that dynamic Bayesian networks are appropriate for such a purpose, since their prediction accuracy was among the highest on new patterns. Our dynamic Bayesian network implementation can accurately recommend the next assembly step in 50% of the cases, but to the detriment of the prediction rate.
Incidence of Phage Capsid Organization on the Resistance to High Energy Proton Beams
Authors: De Plano Laura Maria , Silipigni Letteria , Torrisi Lorenzo , Torrisi Alfio , Cutroneo Mariapompea , Havranek Vladimir , Mackova Anna , Zammuto Vincenzo , Gugliandolo Concetta , Rizzo Maria Giovanna , Guglielmino Salvatore P. P. , Franco Domenico
Published: January 2022

The helical geometry of virus capsid allows simple self-assembly of identical protein subunits with a low request of free energy and a similar spiral path to virus nucleic acid. Consequently, small variations in protein subunits can affect the stability of the entire phage particle. Previously, we observed that rearrangement in the capsid structure of M13 engineered phages affected the resistance to UV-C exposure, while that to H2O2 was mainly ascribable to the amino acids’ sequence of the foreign peptide. Based on these findings, in this work, the resistance to accelerated proton beam exposure (5.0 MeV energy) of the same phage clones was determined at different absorbed doses and dose rates. Then, the number of viral particles able to infect and replicate in the natural host, Escherichia coli F+, was evaluated. By comparing the results with the M13 wild-type vector (pC89), we observed that 12III1 phage clones, with the foreign peptide containing amino acids favorable to carbonylation, exhibited the highest reduction in phage titer associated with a radiation damage (RD) of 35 × 10−3/Gy at 50 dose Gy. On the other hand, P9b phage clones, containing amino acids unfavorable to carbonylation, showed the lowest reduction with an RD of 4.83 × 10−3/Gy at 500 dose Gy. These findings could improve the understanding of the molecular mechanisms underlying the radiation resistance of viruses
Comparative Study of Three Raspberry Cultivar (Rubus idaeus L.) Leaves Metabolites: Metabolome Profiling and Antioxidant Activities
Authors: Zhang Shunbin , Liu Zhao , Li Xu , Abubaker Mohamed Aamer , Liu Xiaoxiao , Li Zhengdou , Wang Xueqi , Zhu Xinliang , Zhang Ji , Chen Xuelin
Published: January 2022

Raspberry (Rubus idaeus L.), known as one of the famous healthy fruits an d are consumed fresh or processed products all over the world. The antioxidation activity of raspberry fruits as well as leaves have been widely investigated. To better understand the metabolite accumulation mechanisms and to develop different functional cultivars, we performed a non-targeted metabolomics analysis using LC-MS/MS to investigate the contents of existing components from three raspberry cultivars, Autumn Britten, Autumn Bliss, and Red Autumn leaves, respectively. The results show multiple differentially accumulated metabolites among three cultivars, especially for the lipids (α-linolenic acid and eicosatetraenoic acid), amino acids and their derivatives (L-cysteine, Phenylalanine), flavonoids (Kaempferol 3-O-rhamnoside-7-O-glucoside, Quercetin 3-glucoside), and vitamins (Biotin, Thiamine, Vitamin K2), etc. The in vitro cellular antioxidant activities of three raspberry cultivars leaves ethanol extracts (RLEE) were also characterized. Through comparison the superoxide dismutase (SOD), glutathione (GSH), catalase (CAT), and reactive oxygen species (ROS) levels before or after RLEE protection of L929 fibroblast cells upon excessive UVB exposure, we evaluated the antioxidation potentials for all three cultivar RLEEs. It turns out the raspberry Autumn Britten leaf extract holds the greatest potential for protecting the L929 fibroblast cells from UVB induced damage. Our study provides theoretical support for screening of active metabolites from three raspberry cultivars leaves, spanning metabolites’ accumulation to cell damage protection, which could be used to refine bioactivity assessment for different raspberry cultivars suitable for antioxidant products extraction.
Things to Consider When Automatically Detecting Parkinson’s Disease Using the Phonation of Sustained Vowels: Analysis of Methodological Issues
Authors: Ozbolt Alex S. , Moro-Velazquez Laureano , Lina Ioan , Butala Ankur A. , Dehak Najim
Published: January 2022

Diagnosing Parkinson’s Disease (PD) necessitates monitoring symptom progression. Unfortunately, diagnostic confirmation often occurs years after disease onset. A more sensitive and objective approach is paramount to the expedient diagnosis and treatment of persons with PD (PwPDs). Recent studies have shown that we can train accurate models to detect signs of PD from audio recordings of confirmed PwPDs. However, disparities exist between studies and may be caused, in part, by differences in employed corpora or methodologies. Our hypothesis is that unaccounted covariates in methodology, experimental design, and data preparation resulted in overly optimistic results in studies of PD automatic detection employing sustained vowels. These issues include record-wise fold creation rather than subject-wise; an imbalance of age between the PwPD and control classes; using too small of a corpus compared to the sizes of feature vectors; performing cross-validation without including development data; and the absence of cross-corpora testing to confirm results. In this paper, we evaluate the influence of these methodological issues in the automatic detection of PD employing sustained vowels. We perform several experiments isolating each issue to measure its influence employing three different corpora. Moreover, we analyze if the perceived dysphonia of the speakers could be causing differences in results between the corpora. Results suggest that each independent methodological issue analyzed has an effect on classification accuracy. Consequently, we recommend a list of methodological steps to be considered in future experiments to avoid overoptimistic or misleading results.
An Improved Dingo Optimization Algorithm Applied to SHE-PWM Modulation Strategy
Authors: Almazán-Covarrubias Juan H. , Peraza-Vázquez Hernán , Peña-Delgado Adrián F. , García-Vite Pedro Martín
Published: January 2022

This paper presents a modification to the dingo optimization algorithm (mDOA) to solve the non-linear set of equations of the selective harmonic elimination (SHE) control technique widely applied in multilevel inverters. In addition, said modification is conducted to the survival criteria by including a local search to provide a better balance when replacing vectors (dingoes) with a low survival rate. The proposed method is also benchmarked with some unimodal functions to illustrate its better exploitation capabilities. Finally, the SHE optimization results were calculated and compared with three well-known state-of-the-art metaheuristics, where the modified version of the dingo optimization algorithm showed very competitive results. The significant difference between the mDOA results and the rest of the algorithms is determined by the Wilcoxon rank-sum non-parametric statistical test with a 5% degree of significance. The p-values confirm the meaningful advantage of the mDOA compared to other bio-inspired algorithms for many modulation indexes. Experimentally, the proposed algorithm is validated through the implementation of a three-phase 11-level inverter.
Stride Length Impacts on Sagittal Knee Biomechanics in Flat Ground Baseball Pitching
Authors: Ramsey Daniel K. , Crotin Ryan L.
Published: January 2022

Coordinated lower extremity biomechanics are altered in response to changes in stride length, influencing the kinetic chain that potentially induces compensatory throwing mechanics throughout the baseball pitching cycle. The respective sagittal knee dynamic profiles, for both the stride (lead) and drive (trail) leg, were analyzed during flat ground baseball pitching to determine whether the stride length variation elicits compensatory drive and stride leg knee joint kinematics, kinetics, and joint powers. Using a randomized cross-over design, a cohort of 19 healthy skilled competitive pitchers from collegiate and high school travel programs from across Western New York were assigned to throw 2 simulated 80 pitch games at ±25% of their desired stride length. An integrated motion capture system with two force plates and a radar gun tracked each throw. Pairwise comparisons at hallmark events and phases identified significantly different sagittal knee dynamics for both the drive and stride leg between the stride length conditions. During the acceleration phase, the drive knee moments between the stride length conditions demonstrated differences in power generation and absorption. Longer strides allowed for greater knee propulsion dynamics, exemplified by eccentric drive knee extensor moments with a concomitant power absorption that slowed the rate of drive knee flexion (p ≤ 0.001). Conversely, shorter strides generated power through concentric knee flexor moments that increased the rate of drive knee flexion (p ≤ 0.001). Stride knee extensor moments and power generation during the acceleration phase were also significantly higher with shorter strides (p ≤ 0.05). Adapted knee joint dynamics may offer insights into stride length optimization, training, and injury prevention strategies.
Steric, Activation Method and Solvent Effects on the Structure of Paddlewheel Diruthenium Complexes
Authors: Delgado-Martínez Patricia , Moreno-Martínez Luis , González-Prieto Rodrigo , Herrero Santiago , Priego José L. , Jiménez-Aparicio Reyes
Published: January 2022

Conventional heating and solvothermal synthetic methods (with or without microwave activation) have been used to study the reaction of o-, m- and p-methoxybenzoic acid with [Ru2Cl(μ-O2CMe)4]. The tetrasubstituted series [Ru2Cl(µ-O2CC6H4-R)4], with R = o-OMe, m-OMe and p-OMe, has been prepared by the three procedures. Depending on the synthetic method and the experimental conditions, three compounds have been isolated (1a, 1b, 1c) with the o-methoxybenzoate ligand. However, with the m- and p-methoxybenzoate ligands, only the complexes 2 and 3 have been obtained, respectively. Compound 1a, with stoichiometry [Ru2Cl(µ-O2CC6H4-o-OMe)4]n, shows a polymeric structure with the chloride ions bridging the diruthenium units to form linear chains. Compounds 2 and 3, with the same stoichiometry, predictably form zig-zag chains in accordance with their insolubility and their magnetic measurements. Compound 1b, [Ru2Cl(µ-O2CC6H4-o-OMe)4(EtOH)], is a discrete molecular species with a chloride ion and one ethanol molecule occupying the axial positions of the dimetallic unit. Compound 1c is a cation-anion complex, [Ru2(µ-O2CC6H4-o-OMe)4(MeOH)2][Ru2Cl2(µ-O2CC6H4-o-OMe)4]. The cationic complex has two solvent molecules at the axial positions whereas the anionic complex has two chloride ligands at these positions. Complexes have been characterized by elemental analyses, mass spectrometry and IR and UV-vis-NIR spectroscopies. A magnetic study of complexes 1a, 1b, 2 and 3 have also been carried out. The crystal structure of compounds 1b and 1c have been solved by single X-ray crystal methods.
The Realization of ZnO Nanowires Interconnection through Femtosecond Laser Irradiation of Ag Nanoparticles Solder
Authors: Guan Yanchao , Wang Genwang , Zhao Shouxin , Wang Lianfu , Ding Ye , Yang Lijun
Published: January 2022

Nanowire interconnection is the basis for the construction and integration of micro-nano functional devices. But so far, it is still difficult to achieve a reliable interconnection of metal oxide nanowires. This letter proposes an approach for soldering ZnO nanowires through femtosecond laser irradiation of Ag nanoparticles solder. In this paper, the effect of femtosecond laser fluence and irradiation time on the morphology of Ag solders and the interconnection state of ZnO nanowires are studied, respectively. The I-V electrical characterization of nanowire interconnection before and after soldering is completed. The results demonstrate that ZnO nanowires achieve better interconnection. The UV light response of the ZnO-Ag-ZnO interconnection structure after soldering is investigated. The approach confirms the effectiveness of a femtosecond irradiated metal nanoparticles solder to achieve metal oxide interconnection, offering the prospect of more metal oxide nanowires interconnection and device development.
Characteristics of Local Geomagnetic Field Variations and the Tectonic Stress Field Adjacent to the 21 May 2021, Ms 6.4 Yangbi Earthquake, Yunnan, China
Authors: Wang Zhendong , Ni Zhe , Chen Shuanggui , Su Shupeng , Yuan Jiehao
Published: January 2022

The tectonic processes leading up to an earthquake and the occurrence of the earthquake itself will cause local changes in the geophysical field (geomagnetic field, stress field, etc.). In this paper, the variation characteristics of the tectonic stress field (TSF) and local geomagnetic field (LGF) before and after the Yangbi Ms 6.4 earthquake are studied. The regional stress tensor damping inversion method was used to invert the TSF using focal mechanism solutions (FMSs). The change characteristics of the TSF before and after the earthquake were analyzed. An annual variation model of the LGF was constructed, and the variation of the horizontal vector was analyzed. The azimuth and plunge of the maximum principal compressive stress axis of the TSF in the epicentral region before and after the earthquake were −4.4° and 2.7°, 172.7° and 6.6°, respectively. The variations in the declination, inclination and total intensity of the epicenter one year before and one month after the earthquake were −0.20′ (0.07′), 0.29′ (−0.12′), and −1.7 nT (−1.9 nT), respectively. The epicenter is located at the boundary of the “weak variation region” of the horizontal vector. This research is of great significance concerning the TSF background and incubation mechanism of earthquakes.
FUXYA2020: A Low-Cost Homemade Portable EDXRF Spectrometer for Cultural Heritage Applications
Authors: Ruschioni Giulia , Micheletti Francesca , Bonizzoni Letizia , Orsilli Jacopo , Galli Anna
Published: January 2022

The project FUXYA2020 was intended to design and prototype a low-cost basic energy dispersive X-ray fluorescence spectrometer for all those cases where there is not enough financial support to buy a commercial device. Indeed, home-made instruments are ideal when funds are low but constant over the years, as this approach allows the costs to be spread over a longer period of time. The FUXYA2020 was intended mostly for cultural heritage (CH) applications: we optimized the geometry to meet the requirements for both low Z matrix objects, such as glasses and ceramics, and medium-high Z materials, such as metals; besides, we designed a positioning system through Arduino components to obtain good results and repeatability for samples with a complex geometry. The FUXYA2020’s performance was tested both for qualitative and quantitative analyses, the former on pigment layers, and the latter on gold-based certified alloys, exploiting Axil-QXAS software for data elaboration. The classification of ancient ceramics based on multivariate analysis obtained through R environment was also carried out. The qualitative data on pigments have also been compared with the same data obtained by a commercial XRF spectrometer, demonstrating how our very simple and inexpensive prototype can be of great help for a rapid and reliable characterization of cultural heritage materials whenever commercial devices are unaffordable.
Stochastic Strength Analyses of Screws for Femoral Neck Fractures
Authors: Frydrýšek Karel , Šír Milan , Pleva Leopold , Szeliga Jiří , Stránský Jan , Čepica Daniel , Kratochvíl Jiří , Koutecký Jan , Madeja Roman , Dědková Kateřina Peterek , Stančeková Dana
Published: January 2022

This paper represents a multidisciplinary approach to biomechanics (medicine engineering and mathematics) in the field of collum femoris fractures, i.e., of osteosyntheses with femoral/cancellous screws with full or cannulated cross-sections. It presents our new numerical model of femoral screws together with their stochastic (probabilistic, statistical) assessment. In the first part of this article, the new simple numerical model is presented. The model, based on the theory of planar (2D) beams on an elastic foundation and on 2nd-order theory, is characterized by rapid solution. Bending and compression loadings were used for derivation of a set of three 4th-order differential equations. Two examples (i.e., a stainless-steel cannulated femoral screw and full cross-section made of Ti6Al4V material) are presented, explained, and evaluated. In the screws, the internal shearing forces, internal normal forces, internal bending moments, displacement (deflections), slopes, and mechanical stresses are calculated using deterministic and stochastic approaches. For the stochastic approach and a “fully” probabilistic reliability assessment (which is a current trend in science), the simulation-based reliability assessment method, namely, the application of the direct Monte Carlo Method, using Anthill software, is applied. The probabilities of plastic deformations in femoral screws are calculated. Future developments, which could be associated with different configurations of cancellous screws, nonlinearities, experiments, and applications, are also proposed.
Trend Pattern of Heavy and Intense Rainfall Events in Colombia from 1981–2018: A Trend-EOF Approach
Authors: Cerón Wilmar L. , Andreoli Rita V. , Kayano Mary T. , Canchala Teresita , Ocampo-Marulanda Camilo , Avila-Diaz Alvaro , Antunes Jean
Published: January 2022

The Andes mountain range divides Colombia into various climatic regions over the country, as the Andean, Caribbean, Pacific, Amazon, and Orinoco regions. Given this scenario, knowing the current change in total precipitation and their extremes values are relevant. In this study, the main goal is to assess the spatio-temporal trends of heavy and intense rainfall at a seasonal scale during the last 38 years (1981–2018) using the trend empirical orthogonal function (TEOF). An increase in maximum precipitation during five consecutive days (RX5day), Simple daily intensity index (SDII), and the number of days with precipitation above 20 mm (R20mm) and 30 mm (R30mm) during December–February and March–May was observed in most of the Colombian territory, except for the Amazon region for RX5day. A decrease in total rainfall in June–August was observed in the Andean, the Caribbean, and southern Pacific regions, while, in the northern Pacific, it increased, consistent with the trend patterns of RX5day, SDII, and R20mm. During September–November, there was a reduction in rainfall in the Amazon region and the South Pacific, and an increase in RX5day, SDII, R20mm, and R30mm in the Andean, the Caribbean, and North Pacific regions. The TEOF showed more pronounced spatial trend patterns than those obtained with the traditional Mann–Kendall test. The findings offer a better understanding of the climate extremes impacts in tropical latitudes and help planners to implement measures against the future effects of climate change.
Satellite Monitoring of the Dust Storm over Northern China on 15 March 2021
Authors: Luo Jingning , Huang Fuxiang , Gao Song , Liu Song , Liu Ruixia , Devasthale Abhay
Published: January 2022

Northern China was hit by a severe dust storm on 15 March 2021, covering a large area and bring devastating impact to a degree that was unprecedented in more than a decade. In the study, we carried out a day-and-night continuous monitoring to the path of the moving dust, using multi-spectral data from the Chinese FY-4A satellite combined with the Japanese Himawary-8 from visible to near-infrared, mid-infrared and far-infrared bands. We monitored the whole process of the dust weather from the occurrence, development, transportation and extinction. The HYSPLIT(Hybrid Single Particle Lagrangian Integrated Trajectory) backward tracking results showed the following two main sources of dust affecting Beijing during the north China dust storm: one is from western Mongolia; the other is from arid and semi-arid regions of northwest of China. Along with the dust storm, the upper air mass, mainly from Siberia, brought a significant decrease in temperature. The transport path of the dust shown by the HYSPLIT backward tracking is consistent with that revealed by the satellite monitoring. The dust weather, which originated in western Mongolia, developed into the “3.15 dust storm” in north China, lasting more than 40 h, with a transport distance of 3900 km, and caused severe decline in air quality in northern China, the Korean peninsula and other regions. It is the most severe dust weather in the past 20 years in east Asia.