bluuebunny/crossref_metadata_2025 huggingface dataset:
Permeability profiling of all 13 Arabidopsis PIP aquaporins using a high throughput yeast approach
Authors: Groszmann Michael , De Rosa Annamaria , Chen Weihua , Qiu Jiaen , McGaughey Samantha A , Byrt Caitlin S. , Evans John R
Published: May 2021

Plant aquaporins have many more functions than just transporting water. Within the diversity of plant aquaporins are isoforms capable of transporting signaling molecules, nutrients, metalloids and gases. It is established that aquaporin substrate discrimination depends on combinations of factors such as solute size, pore size and polarity, and post-translational protein modifications. But our understanding of the relationships between variation in aquaporin structures and the implications for permeability is limited. High-throughput yeast-based assays were developed to assess diverse substrate permeabilities to water, H2O2, boric acid, urea and Na+. All 13 plasma membrane intrinsic proteins (PIPs) from Arabidopsis (AtPIPs) were permeable to both water and H2O2, although their effectiveness varied, and none were permeable to urea. AtPIP2 isoforms were more permeable to water than AtPIP1s, while AtPIP1s were more efficient at transporting H2O2with AtPIP1;3 and AtPIP1;4 being the most permeable. Among the AtPIP2s, AtPIP2;2 and AtPIP2;7 were also permeable to boric acid and Na+. Linking AtPIP substrate profiles with phylogenetics and gene expression data enabled us to align substrate preferences with known biological roles of AtPIPs and importantly guide towards unidentified roles hidden by functional redundancy at key developmental stages and within tissue types. This analysis positions us to more strategically testin plantaphysiological roles of AtPIPs in order to unravel their complex contributions to the transport of important substrates, and secondly, to resolve links between aquaporin protein structure, substrate discrimination, and transport efficiency.

One sentence summary

Yeast based high throughput assays were developed to assess the permeability of each Arabidopsis PIP aquaporin isoform to water, H2O2, boric acid, urea and sodium.
Multi-color 4D superresolution light-sheet microscopy reveals organelle interactions at isotropic 100-nm resolution and sub-second timescales
Authors: Zhao Yuxuan , Zhang Meng , Zhang Wenting , Liu Qing , Wang Peng , Chen Rong , Fei Peng , Zhang Yu-Hui
Published: May 2021

Long-term visualization of the dynamic organelle-organelle or protein-organelle interactions throughout the three-dimensional space of whole live cells is essential to better understand their functions, but this task remains challenging due to the limitations of existing three-dimensional fluorescence microscopy techniques, such as an insufficient axial resolution, low volumetric imaging rate, and photobleaching. Here, we present the combination of a progressive deep-learning superresolution strategy with a dual-ring-modulated SPIM design capable of visualizing the dynamics of intracellular organelles in live cells for hours at an isotropic spatial resolution of ∼100 nm in three dimensions and a temporal resolution up to ∼17 Hz. With a compelling spatiotemporal resolution, we substantially reveal the complex spatial relationships and interactions between the endoplasmic reticulum (ER) and mitochondria throughout live cells, providing new insights into ER-mediated mitochondrial division. We also localized the motion of Drp1 oligomers in three dimensions and observed Drp1-mediated mitochondrial branching for the first time.
Ariadne: Synthetic Long Read Deconvolution Using Assembly Graphs
Authors: Mak Lauren , Meleshko Dmitry , Danko David C. , Barakzai Waris N. , Maharjan Salil , Belchikov Natan , Hajirasouliha Iman
Published: May 2021

Synthetic Long Read (SLR) sequencing techniques such as UST’s TELL-Seq, and Loop Genomics’ LoopSeq combine 3′barcoding with standard short-read sequencing to expand the range of linkage resolution from hundreds to tens of thousands of base-pairs. However, the lack of a 1:1 correspondence between a long fragment and a 3′unique molecular identifier (UMI) confounds the assignment of linkage between short-reads. We introduce Ariadne, a novel assembly graph-based SLR deconvolution algorithm, that can be used to extract single-species read-clouds from SLR datasets to improve the taxonomic classification andde novoassembly of complex populations, such as metagenomes.
Efficient CRISPR/Cas-mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties
Authors: Hahn Florian , Loures Laura Sanjurjo , Sparks Caroline A. , Kanyuka Kostya , Nekrasov Vladimir
Published: May 2021

The CRISPR/Cas technology has recently become a molecular tool of choice for gene function studies in plants as well as crop improvement. Wheat is a globally important staple crop with a well annotated genome and there is plenty of scope for improving its agriculturally important traits using genome editing technologies, such as CRISPR/Cas. As part of this study we targeted three different genes in hexaploid wheat Triticum aestivum: TaBAK1-2 in the spring cultivar Cadenza as well as Ta-eIF4E and Ta-eIF(iso)4E in winter cultivars Cezanne, Goncourt and Prevert. The primary transgenic lines carrying CRISPR/Cas-induced indels were successfully generated for all targeted genes. As winter wheat varieties are generally less amenable to genetic transformation, the successful experimental methodology for transformation and genome editing in winter wheat presented in this study will be of interest to the research community working with this crop.
Can the lack of fibrillar form of alpha-synuclein in Lewy bodies be explained by its catalytic activity?
Authors: Kuznetsov Ivan A. , Kuznetsov Andrey V.
Published: May 2021

Finding the causative pathophysiological mechanisms for Parkinson’s disease (PD) is important for developing therapeutic interventions. Until recently, it was believed that Lewy bodies (LBs), the hallmark of PD, are mostly composed of alpha-synuclein (α-syn) fibrils. Recent results (Shahmoradian et al., Lewy pathology in Parkinson’s disease consists of crowded organelles and lipid membranes, Nature Neuroscience 22 (2019) 1099-1109) demonstrated that the fibrillar form of α-syn is lacking from LBs. Here we propose that this surprising observation can be explained by the catalytic activity of the fibrillar form of α-syn. We assumed that α-syn fibrils catalyze the formation of LBs, but do not become part of them. We developed a mathematical model based on this hypothesis. By using the developed model, we investigated the consequences of this hypothesis. In particular, the model suggests that the long incubation time of PD can be explained by a two-step aggregation process that leads to its development: (i) aggregation of monomeric α-syn into α-syn oligomers and fibrils and (ii) clustering of membranebound organelles, which may cause disruption of axonal trafficking and lead to neuron starvation and death. The model shows that decreasing the rate of destruction of α-syn aggregates in somatic lysosomes accelerates the formation of LBs. Another consequence of the model is the prediction that removing α-syn aggregates from the brain after the aggregation of membrane-bound organelles into LBs has started may not stop the progression of PD because LB formation is an autocatalytic process; hence, the formation of LBs will be catalyzed by aggregates of membrane-bound organelles even in the absence of α-syn aggregates. The performed sensitivity study made it possible to establish the hierarchy of model parameters with respect to their effect on the formation of vesicle aggregates in the soma.
Rapid generation of conditional knockout mice using the CRISPR-CAS9 system and electroporation for neuroscience research
Authors: Nishizono Hirofumi , Hayano Yuki , Nakahata Yoshihisa , Ishigaki Yasuhito , Yasuda Ryohei
Published: May 2021

The Cre/Loxp-based conditional knockout technology is a powerful tool for gene function analyses by allowing region-time-specific gene manipulation. However, inserting a pair of LoxP cassettes for generating conditional knock-out can be technically challenging and thus time- and resource-consuming. This study proposes an efficient, low-cost method to generate floxed mice using the in vitro fertilization and the CRISPR-Cas9 system over two consecutive generations. This method allowed us to produce floxed mice targeting exon 5 to exon 6 of CaMK1 in a short period, 125 days, using only 16 mice. The efficiency of generating floxed mice was 10%, significantly higher than the conventional ES cell-based method. We directly edited the genome of C57BL/6N fertilized eggs, our target genetic background, to eliminate additional backcrossing steps. We confirmed that the genome of this floxed mouse is responsive to Cre protein. This low-cost, highly efficient method for generating conditional knock-out will facilitate comprehensive, tissue-specific genome analyses.
Distinct states of proinsulin misfolding in MIDY
Authors: Haataja Leena , Arunagiri Anoop , Hassan Anis , Regan Kaitlin , Tsai Billy , Dhayalan Balamurugan , Weiss Michael A. , Liu Ming , Arvan Peter
Published: May 2021

A precondition for efficient proinsulin export from the endoplasmic reticulum (ER) is that proinsulin meets ER quality control folding requirements, including formation of the Cys(B19)-Cys(A20) “interchain” disulfide bond, facilitating formation of the Cys(B7)-Cys(A7) bridge. The third proinsulin disulfide, Cys(A6)-Cys(A11), is not required for anterograde trafficking, i.e., a “lose-A6/A11” mutant [Cys(A6), Cys(A11) both converted to Ser] is well secreted. Nevertheless, an unpaired Cys(A11) can participate in disulfide mispairings, causing ER retention of proinsulin. Among the many missense mutations causing the syndrome of MutantINSgene-induced Diabetes of Youth (MIDY), all seem to exhibit perturbed proinsulin disulfide bond formation. Here we have examined a series of seven MIDY mutants [including G(B8)V, Y(B26)C, L(A16)P, H(B5)D, V(B18)A, R(Cpep+2)C, E(A4)K], six of which are essentially completely blocked in export from the ER in pancreatic β-cells. Three of these mutants, however, must disrupt the Cys(A6)-Cys(A11) pairing to expose a critical unpaired cysteine thiol perturbation of proinsulin folding and ER export, because when introduced into the proinsulin lose-A6/A11 background, these mutants exhibit native-like disulfide bonding and improved trafficking. This maneuver also ameliorates dominant-negative blockade of export of co-expressed wild-type proinsulin. A growing molecular understanding of proinsulin misfolding may permit allele-specific pharmacological targeting for some MIDY mutants.
Inhibition of granuloma triglyceride synthesis imparts control ofMycobacterium tuberculosisthrough curtailed inflammatory responses
Authors: Dawa Stanzin , Menon Dilip , Arumugam Prabhakar , Bhaskar Akash Kumar , Mondal Moumita , Rao Vivek , Gandotra Sheetal
Published: May 2021

Lipid metabolism plays a complex and dynamic role in host-pathogen interaction duringMycobacterium tuberculosisinfection. While bacterial lipid metabolism is key to the success of the pathogen, the host also offers a lipid rich environment in the form of necrotic caseous granulomas, making this association beneficial for the pathogen. Accumulation of the neutral lipid triglyceride, as lipid droplets at the cellular cuff of necrotic granulomas, is a peculiar feature of pulmonary tuberculosis. The role of triglyceride synthesis in the TB granuloma and its impact on the disease outcome has not been studied in detail. Here, we identified diacylglycerol O-acyltransferase 1 (DGAT1) to be essential for accumulation of triglyceride in necrotic TB granulomas using the C3HeB/FeJ murine model of infection. Treatment of infected mice with a pharmacological inhibitor of DGAT1 (T863) led to reduction in granuloma triglyceride levels and bacterial burden. A decrease in bacterial burden was associated with reduced neutrophil infiltration and degranulation, and a reduction in several pro-inflammatory cytokines including IL1β, TNFα, IL6, and IFNβ. Triglyceride lowering impacted eicosanoid production through both metabolic re-routing and via transcriptional control. Our data suggests that manipulation of lipid droplet homeostasis may offer a means for host directed therapy in Tuberculosis.
General finite-element framework of the Virtual Fields Method in Nonlinear Elasticity
Authors: Mei Yue , Liu Jiahao , Guo Xu , Zimmerman Brandon , Nguyen Thao D. , Avril Stéphane
Published: May 2021

This paper presents a method to derive the virtual fields for identifying constitutive model parameters using the Virtual Fields Method (VFM). The VFM is an approach to identify unknown constitutive parameters using deformation fields measured across a given volume of interest. The general principle for solving identification problems with the VFM is first to derive parametric stress field, where the stress components at any point depend on the unknown constitutive parameters, across the volume of interest from the measured deformation fields. Applying the principle of virtual work to the parametric stress fields, one can write scalar equations of the unknown parameters and solve the obtained system of equations to deduce the values of unknown parameters. However, no rules have been proposed to select the virtual fields in identification problems related to nonlinear elasticity and there are multiple strategies possible that can yield different results. In this work, we propose a systematic, robust and automatic approach to reconstruct the systems of scalar equations with the VFM. This approach is well suited to finite-element implementation and can be applied to any problem provided that full-field deformation data are available across a volume of interest. We also successfully demonstrate the feasibility of the novel approach by multiple numerical examples. Potential applications of the proposed approach are numerous in biomedical engineering where imaging techniques are commonly used to observe soft tissues and where alterations of material properties are markers of diseased states.

List of symbols
Minimal functional alignment of ventromedial prefrontal cortex intracranial EEG signals during naturalistic viewing
Authors: Xie Tiankang , Cheong Jin Hyun , Manning Jeremy R. , Brandt Amanda M. , Aronson Joshua P. , Jobst Barbara C. , Bujarski Krzysztof A. , Chang Luke J.
Published: May 2021

The ventromedial prefrontal cortex (vmPFC) has been thought to play an important role in processing endogenous information such as generating subjective affective meaning. Unlike sensory cortex, which processes exogenous information about the external world similarly across individuals, prior work has posited that vmPFC activity may be idiosyncratic to each individual, even when exposed to the same external stimulus. In this study, we recorded local field potentials (LFPs) from intracranial stereotactic electrodes implanted in patients with intractable epilepsy while they watched an emotionally engaging television show episode and evaluated temporal synchronization of these signals across participants in auditory cortex and vmPFC. Overall, we observed markedly lower intersubject synchronization of signals recorded from electrodes implanted in vmPFC compared to auditory cortex. A subset of patients, however, appeared to share similar vmPFC states during the more emotionally salient scenes. This work suggests that the vmPFC is involved in processing affective responses to ongoing experience in a state-like manner, but the specific states and temporal sequences are idiosyncratic to each individual, even when viewing the same television episode.
Comprehensive analysis of meiosis-derived cDNA libraries reveals gene isoforms and mitochondrial proteins important for competitive fitness
Authors: Sing Tina L. , Conlon Katie , Lu Stephanie H. , Madrazo Nicole , Barker Juliet C. , Hollerer Ina , Brar Gloria A. , Sudmant Peter H. , Ünal Elçin
Published: May 2021

Gametogenesis is a highly regulated and dynamic developmental program where a diploid progenitor cell differentiates into haploid gametes, the precursors for sexual reproduction. During meiosis, several pathways converge to initiate ploidy reduction and organelle remodelling to render gametes competent for zygote formation and subsequent organismal development. Additionally, meiosis inherently rejuvenates the newly formed gametes resulting in lifespan resetting. Here, we construct five stage-specific, inducible meiotic cDNA libraries that represent over 84% of the yeast genome. We employ computational strategies to detect stage-specific meiotic transcript isoforms in each library and develop a robust screening pipeline to test the effect of each cDNA on competitive fitness. Our multi-day proof-of-principle time course reveals gene isoforms that are important for competitive fitness as well as mitochondrial proteins that cause dose-dependent disruption of respiration. Together, these novel meiotic cDNA libraries provide an important resource for systematically studying meiotic genes and gene isoforms in future studies.

HIGHLIGHTS

Construction of five stage-specific, inducible meiotic cDNA libraries in budding yeast that collectively represent 5563 genes, which is over 84% of the genome

Analysis of the cDNA libraries reveal the presence of meiosis-specific transcript isoforms that are largely uncharacterized

Development of a robust gain-of-function screening pipeline identifies previously characterized genes and novel gene isoforms important for competitive fitness

Multi-day proof-of-principle screen reveals mitochondrial proteins that cause dosage-specific respiration defects
Managing teaching and learning: integrating instructional and transformational leadership in South African schools context
Authors: Shava George , Heystek Jan
Published: May 2021

Purpose

The purpose of this study was to examine the integration of instructional and transformational leadership models of leadership in sustaining quality teaching and learning in schools. The study sought to establish how principals integrate instructional and transformational leadership in enhancing learner performance.

Design/methodology/approach

The study employed qualitative methods of collecting and analysing data. The principal sources of data were six face-to-face semi-structured interview questions with school principals from selected schools in rural South Africa. Qualitative evidence was collected from six principals selected through purposive sampling. The selection of participants was based on the criteria that there was evidence of employing instructional and transformational leadership.

Findings

Findings from the study provided evidence that instructional and transformational leadership approaches were used to change under performing schools. There was evidence of individualised consideration and principals supporting teachers through providing rewards and motivation. It was established that principals build a school culture that promotes successful academic improvement. The study showed that the integration of instructional and transformational models of leadership leads to a climate that promotes a culture of teaching and learning.

Research limitations/implications

The study covered six schools in South Africa. Findings from the study have implications that principals are cornerstones to achieving quality teaching and learning in schools.

Practical implications

The study was conducted in schools that were seen to adopt instructional and transformational leadership. This study is among the most important studies that were conducted in South Africa on the role of leadership in enhancing a culture of teaching and learning.

Social implications

The study has critical implications for policy making and influences on school leadership in general and the adoption of strategies, policies and models that can improve teaching and learning. The study highlights the importance of integrating leadership models.

Originality/value

This is an original study conducted in South Africa and data was conducted through face-to-face interviews to seek for opinions from participants in their original settings.
Maintaining Dignity – A case of outsourced paper plant cleaners in Finland
Authors: Kalliola Satu , Niemelä Jukka
Published: May 2021

Outsourcing has gained favor since the 1980s, and Finnish paper companies used it as late as 2006, when a group of female cleaners were outsourced from the case plant of this study. This article focuses on the context of outsourcing, characterized by the bargaining power and choices made by the bargaining parties, the responses of the cleaners over time and the potential theoretical explanations of the outcome. The responses, such as disappointment and anger, mental and physical tiredness, sickness absenteeism, and starting to get adjusted, were interpreted in the frameworks of occupational culture, the job characteristics model, old and new craftsmanship, and relational and transactional psychological contracts. The method was a combination of naturalistic inquiry and abduction. The study points out that more than one theoretical framework was needed to gain an understanding of the situation.
NFC and IoT-based electronic health card for elementary students using sensor fusion method
Authors: Basjaruddin Noor Cholis , Rifqi Fakhrudin Faris , Sudarsa Yana , Noor Fatimah
Published: May 2021

Purpose

In the context of overcoming malnutrition in elementary school children and increasing public awareness of this issue, the Indonesian Government has created a “Card for Healthy School Children” (KMS-AS) program in the form of a paper health card. However, currently, the KMS-AS record data are still written on paper, which is less effective in terms of the health process. An integrated measuring device and an online data-recording system are needed to promote children’s health and facilitate access and transfer of data from one place to another. This study aims to develop NFC and IoT-based KMS-AS using sensor fusion method.

Design/methodology/approach

The results of this study show that an integrated measuring device for weight, height, body temperature and Spo2 level can be connected with mobile and Web applications using IoT technology, facilitating data recording and monitoring of children’s nutritional status. The sensor fusion method was used for the classification of nutritional status and health status, based on the results of measurement tools. Near field communication (NFC) technology was used to facilitate user identification when making measurements.

Findings

The results show that KMS-AS can facilitate classification of nutritional status and children's health status. Measurement and classification data can be monitored via Web and mobile applications. The accuracy of height, weight, body temperature and Spo2 measurements was 98.21%, 98.59%, 98.93% and 98.93%, respectively.

Originality/value

In this research, the authors successfully produced a system using sensor fusion method for measuring body weight, height, temperature and Spo2 level, which is integrated and can be connected to mobile applications and the Web using the IoT and NFC.
Human resource management (HRM) in the performance measurement and management (PMM) domain: a bibliometric review
Authors: Garengo Patrizia , Sardi Alberto , Nudurupati Sai Sudhakar
Published: May 2021

Purpose

The literature highlights the key role of human resource management in developing effective organizational performance measurement and management. To understand the state of the art of this role, the paper reviews the literature on human resource management in the performance measurement and management domain.

Design/methodology/approach

The paper conducts a bibliometric literature review on 1,252 articles to identify the prevailing research trends and the conceptual structure of human resource management in the performance measurement and management domain.

Findings

The study highlights a growing number of publications and four themes related to human resource management in performance measurement and management. It also underlines the shift from static to the dynamic performance measurement and management systems within organization which is expected to be more suited to current and future contexts.

Practical implications

The paper highlights the need to manage the identified themes as strategic organizational assets and further develop the strategic dimension of human resource management practices leveraging on project management and information systems.

Originality/value

The paper goes beyond the traditional focus on performance appraisal of human resource management studies and assumes the challenge of connecting two research fields: human resource management and performance measurement and management.
Disappearing natural resources: what flowers tell us about new value chains
Authors: Carton Guillaume , Parigot Julia
Published: May 2021

Purpose

This paper aims to question the capacity of firms embedded in global value chains to manage their natural resources in a sustainable way. Thus, it offers guidelines for more sustainable value chains.

Design/methodology/approach

While business strategies have focused on optimizing natural resource exploitation and on constructing global value chains to face sustainability issues, this study first explains why these strategies are not effective in preventing natural resource depletion. Second, it offers a model for anticipating resource depletion. The cut flower industry constitutes a central case to explain the model. Two other industry cases complement the demonstration.

Findings

To anticipate natural resource depletion and thus improve industry sustainability, firms must shift from the exploitation of endangered natural resources to the use of alternative local ones. This shift, however, encourages firms to reconstruct value chains and rethink how they create value within these new value chains. It also has an impact on firms’ growth strategy: they must replicate value chains on a local scale instead of taking part in global value chains.

Research limitations/implications

The findings rely on illustrations from the cut flower, fishing and textile fiber industries. Generalization to other industries may strengthen the argument.

Originality/value

This study offers a model of sustainable growth for firms willing to anticipate natural resource depletion by offering a shift in value chains. It consists of exploiting alternative natural resources and of rethinking the value offered to consumers. Thus, it goes against current models that merely focus on optimizing natural resource exploitation within global value chains.
Meeting you was a fake: investigating the increase in romance fraud during COVID-19
Authors: Buil-Gil David , Zeng Yongyu
Published: May 2021

Purpose

Romance fraud refers to situations where an individual is deceived for financial gain by someone with whom the victim perceives to be in a romantic relationship. With the increase in internet use, the growth in loneliness and the increasing engagement in online dating sites during COVID-19, opportunities for romance fraud may have suffered an important increase. This paper aims to analyse changes in romance fraud, loneliness and internet use during the pandemic.

Design/methodology/approach

Data about romance fraud reported to the police in the UK, and survey data recorded by the Understanding Society longitudinal survey, are used to address our research questions. Auto regressive integrated moving average (ARIMA) modelling is used to analyse whether temporal changes observed are an effect of social changes associated with lockdown and stay-at-home orders.

Findings

The analysis shows that cyber-enabled romance fraud experienced a large increase after April 2020, which is greatly above any expected crime variation considering known pre-COVID trends. The increase in romance fraud was more abrupt among young adults than older persons. The results also indicate that only young adults experienced a significant increase in loneliness, while older adults reported a large increase in internet use during COVID.

Originality/value

To the best of the authors’ knowledge, this is a first-of-its-kind paper analysing the effect of rapid social changes on a growing type of cyber-enabled fraud. It is likely that the growth in romance fraud during COVID is due to a combined effect of an increase in internet use and growing loneliness rates experienced by many people during the pandemic.
Lower reversal limit of the European Central Bank deposit rate and sustainability of traditional banking business model
Authors: Gubareva Mariya
Published: May 2021

Purpose

The purpose of this paper is to present an empirical analysis of the European Central Bank (ECB) deposit rate dynamics during 2014–2020, attempting to answer how deep could be cut further this rate without causing persistent yield curve inversions (YCI), i.e. lower yields for longer terms. It addresses the sustainability of the traditional banking and shows that inverted yield curves would require changing the banking-as-usual model to the government-guaranteed long-term-borrowing coupled with short-term-lending. This research poses the question of whether the banking sector should become a public utility.

Design/methodology/approach

The future scenarios of negative interest rate (NIR) behavior are modeled seeking to increase the understanding of NIR environment. Using an event-study design, empirical analyses of the ECB deposit rate cuts on the Euro Over-Night Index Average rates is performed at different maturities.

Findings

This study finds that, starting from the lower limit of 80 basis points below zero, the ECB deposit rate is likely to result in complete YCIs.

Social implications

This paper evidences that moving rates into a more negative territory is likely to be completely counterproductive for banking industry, implying that banking at such conditions would become heavily dependent on governmental support. The results shed light on the interdependence of the banking business, financial monetary policy and welfare of the society, providing policymakers with empirically defined milestones for policy implementations.

Originality/value

This paper clarifies the impact of the ECB deposit rate on the overall shape of yield curves. The novelty of this research resides in investigation of YCI by simulating NIR dynamics.
Employees as influencers: measuring employee brand equity in a social media age
Authors: Smith Donna , Jacobson Jenna , Rudkowski Janice L.
Published: May 2021

Purpose

The practice of frontline employees articulating their brand voice and posting work-related content on social media has emerged; however, employee brand equity (EBE) research has yet to be linked to employees’ social media activity. This paper aims to take a methods-based approach to better understand employees’ roles as influencers. As such, its objective is to operationalize and apply the three EBE dimensions – brand consistent behavior, brand endorsement and brand allegiance – using Instagram data.

Design/methodology/approach

This qualitative research uses a case study of employee influencers at SoulCycle, a leading North American fitness company and examines 100 Instagram images and 100 captions from these influential employees to assess the three EBE dimensions.

Findings

Brand consistent behavior (what employees do) was the most important EBE dimension indicating that employees’ social media activities align with their employer’s values. Brand allegiance (what employees intend to do in the future) whereby employees self-identify with their employer on social media, followed. Brand endorsement (what employees say) was the least influential of the three EBE dimensions, which may indicate a higher level of perceived authenticity from a consumer perspective.

Originality/value

This research makes three contributions. First, it presents a novel measure of EBE using public Instagram data. Second, it represents a unique expansion and an evolution of King et al.’s (2012) model. Third, it considers employees’ work-related content on social media to understand employees’ role as influencers and their co-creation of EBE, which is currently an under-represented perspective in the internal branding literature.
A professional development practice to enhance principals' instructional leadership – enabling and constraining arrangements
Authors: Liljenberg Mette
Published: May 2021

Purpose

The purpose of this paper is to increase the knowledge of principals' professional development (PD) by focusing on the arrangements that shape a PD practice initiated to enhance principals' instructional leadership.

Design/methodology/approach

The paper draws on findings from a three-year PD initiative in a Swedish school district. The data consist of field notes and semi-structured interviews with principals and managers. Theoretically, the paper takes its starting point in the theory of practice architectures and the cultural-discursive, material-economic and social-political arrangements that shape practices.

Findings

Practices for principals' PD require a fine balance to prevent the perception of hierarchical control. Designated time, common artifacts and external expertise are arrangements of practice that enable principals' PD. High expectations and relations between principals and managers both enable and constrain principals' PD. As trustful relations are of particular importance, the implication for managers and others organizing for principals' PD is to make sure that collaborative work settings also become a natural way of working for principals. As building relations takes time, a longer time perspective is also recommended.

Practical implications

The findings have practical implications for educational leaders responsible for organizing PD practices for principals in any context.

Originality/value

This paper adopts a practice theory approach to its study of principals' PD and provide an elaborated illustration of arrangements that enable and constrain principals' PD in collegial settings.
A mixed-methods investigation of mental health stigma, absenteeism and presenteeism among UK postgraduate researchers
Authors: Berry Clio , Niven Jeremy E. , Chapman Laura A. , Valeix Sophie , Roberts Paul E. , Hazell Cassie Marie
Published: May 2021

Purpose

Postgraduate researchers (PGRs) appear to be particularly vulnerable to mental health problems. Mental health-related stigma and discrimination may be endemic within universities, creating a threatening environment that undermines PGRs’ health and well-being. These environmental characteristics may increase PGRs’ absenteeism and presenteeism, attendance behaviours that have great personal and institutional consequences. The study of this issue, however, has been limited to date.

Design/methodology/approach

This was a mixed methods psychological study using cross-sectional data provided by 3,352 UK-based PGRs. Data were collected in a new national survey (U-DOC) led by a British University in 2018–2019. We used structural equation modelling techniques to test associations between workplace mental health-related stigma and discrimination, presenteeism, absenteeism and demographic characteristics. The authors analysed qualitative survey data with framework analysis to deductively and inductively explore associations between workplace culture, stigma and discrimination, and attendance behaviours.

Findings

The authors found that some PGRs report positive perceptions and experiences of the academic mental health-related workplace culture. However, experiences of mental health stigma and discrimination appear widespread. Both quantitative and qualitative results show that experiences of mental health-related stigma are associated with greater absenteeism and presenteeism. People with mental health problems appear especially vulnerable to experiencing stigma and its impacts.

Practical implications

Key implications include recommendations for universities to improve support for PGR mental health, and to encourage taking annual leave and necessary sickness absences, by providing a more inclusive environment with enhanced mental health service provision and training for faculty and administrative staff.

Originality/value

This study presents the first large-scale survey of PGR experiences of mental health-related stigma and discrimination, and their associations with absenteeism and presenteeism.
Which factors influence Demodex proliferation? A retrospective pilot study highlighting a possible role of subtle immune variations and sebaceous gland status
Authors: Forton Fabienne M. N. , De Maertelaer Viviane
Published: May 2021

Demodex folliculorum and brevis are commensal mites that live in low densities in human pilosebaceous follicles as part of the normal adult microbiota, but that give rise to demodicosis and, possibly, rosacea, when they proliferate excessively. This proliferation is favored by various factors, including age, marked immunosuppression, sebaceous gland hyperplasia, and hypervascularization‐related factors. To study possible factors influencing mite proliferation, we explored the effects of different variables on Demodex densities (Dd) in a retrospective study of two groups of subjects selected on the basis of their clinical diagnosis: Demodex+, consisting of subjects with demodicosis or with centro‐facial papulopustules suggesting rosacea (n = 844, mean Dd 263.5 ± 8.9 D/cm2), and Demodex−, consisting of subjects with other facial dermatoses or healthy facial skin (n = 200, mean Dd 2.3 ± 0.4 D/cm2). Demodex densities were measured using two consecutive standardized skin surface biopsies (SSSB1 [superficial] and SSSB2 [deep]) taken from the same facial site on each subject. In the Demodex+ group: the SSSB1 decreased with age in women (p = 0.004), and the SSSB2 increased with age in men (p = 0.001) (the pattern was similar for SSSB1 + 2, but not statistically significant); Dds were lower in those who had received cortisone (either topically or systemically); 13 subjects (1.5%) had known immunosuppression, 62 (7.3%) had hypothyroidism, and in 20 (3.6% of the women) there was a reported link with pregnancy; 78 of the subjects (9.2%) were part of a pair from the same family or household; when associated bacterial infection was suspected, Staphylococcus epidermidis was often isolated. Our results suggest close interactions between the mite, sebaceous gland size and function, and subtle variations of immune status. Potential factors influencing Demodex proliferation should be further investigated, including hypothyroidism, pregnancy, corticosteroid administration, Staphylococcus epidermidis, contagiousity, and genetic background.
Spring phenological escape is critical for the survival of temperate tree seedlings
Authors: Lee Benjamin R. , Ibáñez Inés
Published: May 2021

Understorey plants in deciduous forests often rely on access to ephemeral light availability before the canopy closes in spring and after the canopy reopens in fall, a strategy commonly referred to as phenological escape. Although there is evidence for a relationship between understorey plant phenology and demographic performance, a mechanistic link is still missing.

In this study, we bridged this gap by estimating annual carbon assimilation as a function of foliar phenology and photosynthetic capacity for seedlings of two temperate tree species that commonly co‐occur across eastern North America. We then modelled the relationship between estimated carbon assimilation and observed seedling survival and growth.

Our results indicate that seedlings of both species strongly depend on spring phenological escape to assimilate the majority of their annual carbon budget and that this mechanism significantly affects their likelihood of survival (but not growth). Foliar desiccation also played a strong role in driving patterns of seedling survival, suggesting that water availability will also help shape seedling recruitment dynamics. We found only weak associations between seedling senescence in fall and annual carbon assimilation, suggesting that phenological escape in fall plays a relatively minor role in seedling demographic performance.

Our results indicate that spring phenological escape is critical for survival of these temperate tree species, and thus, any changes to this dynamic associated with climate change could strongly impact these species' recruitment.

A freePlain Language Summarycan be found within the Supporting Information of this article.
A dormant amoeba species can selectively sense and predate on different soil bacteria
Authors: Shu Longfei , He Zhenzhen , Guan Xiaotong , Yang Xueqin , Tian Yuehui , Zhang Siyi , Wu Chenyuan , He Zhili , Yan Qingyun , Wang Cheng , Shi Yijing
Published: May 2021

Soil protists are the invisible majority of soil eukaryotes, which are essential but often forgotten parts of the soil ecosystem. They play key roles in microbial food webs by predating on other soil microbes. However, it is not clear how dormant soil protists sense, recognize and feed on diverse microbial prey.

In this study, we used a soil amoeba, Dictyostelium discoideum, to study selective discrimination and predation of 14 different bacteria. We found that discrimination and sensing of prey in D. discoideum started as early as resting spores. Dictyostelium discoideum had higher hatching rates, formed bigger amoeba plaques and preferred high nutritional value bacteria. The feeding speed of amoeba on various bacteria was constant and was not linked with sensing of prey or bacterial nutritional value. We also found that higher bacterial density decreased predation efficiency, and one species, P. fluorescens, induced a strong density‐dependent inhibition of amoeba spore production.

In conclusion, we find that dormant D. discoideum can selectively sense and predate on different soil bacteria, a process that is likely mediated through active amoeba preference as well as bacterial inhibition. This study provides new insights into the role of protists in shaping soil bacterial communities, and future study needs to assess this in natural soil environments.
Disentangling the influence of water limitation and simultaneous above and belowground herbivory on plant tolerance and resistance to stress
Authors: Mundim Fabiane M. , Vieira‐Neto Ernane H. M. , Alborn Hans , Bruna Emilio M.
Published: May 2021

Plants face multiple biotic and abiotic stressors simultaneously. Many species can tolerate and resist stress, but countermeasures differ between roots and leaves. Since herbivores and environmental conditions modulate costs and benefits of plant defence traits, stress responses are context‐dependent. We examined whole‐plant tolerance and resistance responses to individual and combined effects of above and belowground herbivory under variable water conditions.

We manipulated water availability and access by two common herbivores (Spodoptera exigua caterpillars and Meloidogyne incognita nematodes) to Solanum lycocarpum. Plants were either watered based on historical regional averages or the 30% reduction predicted by IPCC studies. Herbivory treatments included isolated above (AG) and belowground (BG) attacks, simultaneous (AGBG) attacks and no‐herbivory controls. We then parameterized generalized linear mixed‐effects models with data on plant survival, leaf and root biomass accumulation, root complexity and terpenoid concentration.

Foliar herbivory increased terpenoid concentrations in roots relative to no‐herbivory plants under control water but decreased concentrations in both roots and leaves under drought. Similarly, root feeders increased concentrations of terpenoids in leaves under control water but decreased concentrations only in roots under drought. Plants challenged with AGBG herbivory had greater whole‐plant biomass (i.e. tolerance) and lower total concentrations of defensive compounds (i.e. resistance) than plants exposed to no‐herbivore controls, regardless of water conditions. Importantly, the capacity of plants to grow or produce terpenoids changes when herbivory level is considered. In plants exposed to AGBG herbivory, greater nematode infection was related to decreases in whole‐plant biomass and marginal increases in total terpenoid concentration. Ultimately, accounting only for individual AG and BG responses would have led to different conclusions and underestimated the magnitude of S. lycocarpum's compensatory responses. A ‘whole‐plant’ approach revealed that belowground herbivory is the primary driver of tolerance in plants surviving moderate water stress.

Synthesis. Whole‐plant responses to stress in variable environments are complex, and their comprehensive understanding requires accounting for belowground herbivores and root responses.
Inequality and the Chinese elite: Between international convergence and national divergence
Authors: Segal Paul
Published: May 2021

This paper considers the position of the Chinese elite in both national and international contexts. I show that the Chinese elite has converged with the global elite in terms of their incomes, and that many senior Chinese executives are at least as well paid as their counterparts in rich countries. Their insertion into the global elite is further demonstrated by their increasing participation in the World Economic Forum. But this international convergence has been mirrored by national divergence: economic inequality has risen within China, extending the distance between elites and the rest of the population. I show that for top income groups, the relative cost of employing a median wage worker for their personal service has plummeted, increasing their entitlements over labor and thus their capacity to dominate their compatriots. This has been accompanied by a dramatic rise in the reliance of the elite on domestic workers, a traditional symbol of social inequality. Thus the convergence of Chinese elites with their international counterparts has gone hand in hand with both rising economic inequality and rising social inequality within China.
Lives on track? Long‐term earnings returns to selective school placement in England and Denmark
Authors: Birkelund Jesper Fels , Capsada‐Munsech Queralt , Boliver Vikki , Karlson Kristian Bernt
Published: May 2021

We explore the influence of between‐school ability placement at lower secondary education on earnings across the life course in England and Denmark. We go beyond the mid‐career snapshot provided by previous studies by exploiting the availability of four decades worth of earnings data for individuals born in the mid‐1950s. Members of this cohort who were judged to be among the most academically able attended grammar schools in England (19%) and advanced secondary schools (Realskole) in Denmark (51%) prior to the start of comprehensivization. This key difference makes England and Denmark interesting cases for comparison, not least since pro‐selection policies have re‐emerged in England based on the claim that grammar schools lead to better educational and labor market outcomes. Our analysis of the influence of selective school placement on earnings finds little support for this contention. We find that those from socioeconomically disadvantaged backgrounds were strikingly under‐represented in schools ear‐marked for higher ability pupils in both countries, even after taking into account social class differences in measured ability. Our analysis for England finds only modest earnings returns to attending a grammar school, totalling just £39,000 across the life course, while in Denmark the lifetime earnings returns to attending Realskole are somewhat larger (£194,000). Because those from advantaged backgrounds were substantially over‐represented at grammar schools and Realskoles, these returns accrue disproportionately to pupils from more advantaged backgrounds. Lower secondary school placement in Denmark accounts for 40% of the intergenerational reproduction of socioeconomic advantage and disadvantage, more than half of which is due to selection into school types based on socioeconomic background rather than measured ability. Our findings question the wisdom of expanding grammar schools when they appear to do little to improve individuals’ earnings or increase social mobility.