bluuebunny/crossref_metadata_2025 huggingface dataset:
Influencing factors of destination choice of ski tourism enthusiasts: A means-end chain analytical perspective
Authors: Sun Hui , Xie Xia , Gao Jun , Zhang Lei
Published: December 2022

Ski enthusiasts are a unique group of travelers whom many travel businesses and destinations market to. Drawing on a means-end chain analytical perspective, this study explores the main factors affecting the destination choice of ski tourism enthusiasts. Three levels of main factors, including destination attribute, experience consequence, and personal value, were first explored via a soft laddering interview (N = 28), followed by a questionnaire survey (N = 339). The results show that ski resort conditions (e.g., quality of ski tracks and snow quality) are the most important attribute of the destination for ski enthusiasts, with the strongest influence on their experience sequence. In addition to the value needs of positive emotion and self-adjustment, ski tourism enthusiasts also show a strong need for self-development. This result has important guiding significance for the formulation of ski tourism destination marketing strategy.
The effectiveness of Promoting Alternative Thinking Strategies program: A meta-analysis
Authors: Shi Jieping , Cheung Alan C. K. , Ni Aohua
Published: December 2022

Promoting Alternative Thinking Strategies (PATHS) is a widely-used social emotional learning program for preschool and elementary school students. The purpose of this review is to examine its effects, and explore the moderation effects of methodological and implementation features on intervention effectiveness. Using stringent inclusion criteria, 20 qualified studies and 177 effect sizes involving 30,454 participants were included. Results showed that the overall effect size of PATHS was 0.11. In particular, the effect size of PATHS on social emotional skills (ES = 0.16) was the largest compared with other outcome domains, including attitude or relations (ES = 0.08), emotional well-being (ES = 0.02), prosocial behaviors (ES = 0.04), conduct problems (ES = 0.06), and academic performance (ES = 0.05). PATHS had no different impact whether it was implemented in the universal or target contexts. Research design, sample size, and intervention dosage could moderate the effectiveness of PATHS significantly, and dosage was the predominant factor in determining the effects of PATHS. Policy and practical implications were discussed.
Space-valence mapping of social concepts: Do we arrange negative and positive ethnic stereotypes from left to right?
Authors: Kühne K. , Nenaschew K. , Miklashevsky A.
Published: December 2022

Introduction

The body-specificity hypothesis states that in right-handers, positive concepts should be associated with the right side and negative concepts with the left side of the body. Following this hypothesis, our study postulated that negative out-group ethnic stereotypes would be associated with the left side, and positive in-group stereotypes would be associated with the right side.

Methods

The experiment consisted of two parts. First, we measured the spatial mapping of ethnic stereotypes by using a sensibility judgment task, in which participants had to decide whether a sentence was sensible or not by pressing either a left or a right key. The sentences included German vs. Arabic proper names. Second, we measured implicit ethnic stereotypes in the same participants using the Go/No-go Association Task (GNAT), in which Arabic vs. German proper names were presented in combination with positive vs. negative adjectives. Right-handed German native speakers (N = 92) participated in an online study.

Results

As predicted, in the GNAT, participants reacted faster to German names combined with positive adjectives and to Arabic names combined with negative adjectives, which is diagnostic of existing valenced in-and outgroup ethnic stereotypes. However, we failed to find any reliable effects in the sensibility judgment task, i.e., there was no evidence of spatial mapping of positive and negative ethnic stereotypes. There was no correlation between the results of the two tasks at the individual level. Further Bayesian analysis and exploratory analysis in the left-handed subsample (N = 9) corroborated the evidence in favor of null results.

Discussion

Our study suggests that ethnic stereotypes are not automatically mapped in a body-specific manner.
Neurobiological outcomes of cognitive behavioral therapy for obsessive-compulsive disorder: A systematic review
Authors: Poli Andrea , Pozza Andrea , Orrù Graziella , Conversano Ciro , Ciacchini Rebecca , Pugi Daniele , Angelo Nicole Loren , Angeletti Lorenzo Lucherini , Miccoli Mario , Gemignani Angelo
Published: December 2022

Introduction

Obsessive-compulsive disorder (OCD) is characterized by recurrent distressing thoughts and repetitive behaviors, or mental rituals performed to reduce anxiety. Recent neurobiological techniques have been particularly convincing in suggesting that cortico-striatal-thalamic-cortico (CSTC) circuits, including orbitofrontal cortex (OFC) and striatum regions (caudate nucleus and putamen), are responsible for mediation of OCD symptoms. However, it is still unclear how these regions are affected by OCD treatments in adult patients. To address this yet open question, we conducted a systematic review of all studies examining neurobiological changes before and after first-line psychological OCD treatment, i.e., cognitive-behavioral therapy (CBT).

Methods

Studies were included if they were conducted in adults with OCD and they assessed the neurobiological effects of CBT before and after treatment. Two databases were searched: PsycINFO and PubMed for the time frame up to May 2022.

Results

We obtained 26 pre-post CBT treatment studies performed using different neurobiological techniques, namely functional magnetic resonance imaging (fMRI), Positron emission tomography (PET), regional cerebral blood flow (rCBF), 5-HT concentration, magnetic resonance imaging (MRI), magnetic resonance spectroscopy (MRS), Electroencephalography (EEG). Neurobiological data show the following after CBT intervention: (i) reduced activations in OFC across fMRI, EEG, and rCBF; (ii) decreased activity in striatum regions across fMRI, rCBF, PET, and MRI; (iii) increased activations in cerebellum (CER) across fMRI and MRI; (iv) enhanced neurochemical concentrations in MRS studies in OFC, anterior cingulate cortex (ACC) and striatum regions. Most of these neurobiological changes are also accompanied by an improvement in symptom severity as assessed by a reduction in the Y-BOCS scores.

Conclusion

Cognitive-behavioral therapy seems to be able to restructure, modify, and transform the neurobiological component of OCD, in addition to the clinical symptoms. Nevertheless, further studies are necessary to frame the OCD spectrum in a dimensional way.
Interplay of health literacy, healthcare access and health behaviors with oral health status among older persons
Authors: Amoah Padmore Adusei , Boateng Millicent Ofori , Koduah Adwoa Owusuaa , Acheampong Princess Ruhama
Published: December 2022

This study contributes to the ongoing debate on social determinants of oral health of older persons. Specifically, it examines the direct and indirect effects of health literacy and access to healthcare on oral health status of older persons. The study also investigates whether general health status and health behavior (routine medical check-ups) explain the association of health literacy and healthcare access with oral health status. The gender dimensions of these relationships are also explored. Data were derived from 522 participants aged 50 years and older located in five regions in Ghana. Path analyses in structural equation modeling (SEM) were used to analyse the data. General health status (β = −0.049, p < 0.005), medical check-up (β = 0.124, p < 0.01), and health literacy (β = 0.133, p < 0.01) were positively associated with oral health status. General health status mediated the positive relationship between health literacy and oral health status (β = 0.048, p < 0.01). General health status (β = 0.016, p < 0.05) and medical check-ups (β = 0.025, p < 0.05) mediated the association between access to healthcare and oral health status. The mediational role of routine medical check-up in the association between access to healthcare and oral health status was significantly stronger (B = 0.063, p < 0.01) among men (β = 0.051, p < 0.01) than women (β = 0.003, p > 0.05). Analyses of oral health issues among older persons in Ghana and settings alike must recognize the complex interplay among critical social determinants to initiate pragmatic health and social policy interventions.
Composite materials for innovative urban farming of alternative food sources (macroalgae and crickets)
Authors: Fricke Anna , Psarianos Marios , Sabban Jakob , Fitzner Maria , Reipsch Riccardo , Schlüter Oliver K. , Dreyer Christian , Vogt Julia H.-M. , Schreiner Monika , Baldermann Susanne
Published: December 2022

Facing an inexorable growth of the human population along with substantial environmental changes, the assurance of food security is a major challenge of the present century. To ensure responsible food consumption and production (SDG 12), new approaches in the food system are required. Thus, environmentally controlled, sustainable production of alternative food sources are of key interest for both urban agriculture and food research. To face the current challenge of integrating food production systems within existing structures, multidisciplinary discourses are required. Here, we bring together novel technologies and indoor farming techniques with the aim of supporting the development of sustainable food production systems. For this purpose, we investigated the feasibility of 10 composite materials for their innovative use as structural support in macroalgal cultivation (settlement substrates) and cricket rearing (housing). Considering material resistance, rigidity, and direct material-organism interactions, the bio-based composite polylactic acid (PLA) was identified as a suitable material for joint farming. For macroalgae cultivation, PLA sustained the corrosive cultivation conditions and provided a suitable substrate without affecting the macroalgal physiology or nutritional composition (carotenoids and chlorophylls). For cricket rearing, PLA provided a suitable and recyclable shelter, which was quickly accepted by the animals without any observed harm. In contrast, other common composite components like phenolic resin or aramid were found to be unsuitable due to being harmful for the cultivated organisms or instable toward the applied sterilization procedure. This multidisciplinary study not only provides profound insights in the developing field of urban indoor food production from a new perspective, but also bridges material science and farming approaches to develop new sustainable and resilient food production systems.
Nutritive values and phytochemical compositions of edible indigenous plants in Thailand
Authors: Sridonpai Piyanut , Kongprapun Pichakorn , Sungayuth Nongnuch , Sukprasansap Monruedee , Chimkerd Chanika , Judprasong Kunchit
Published: December 2022

Indigenous edible plants are important components of food systems that are linked to food security and are important sources of nutrients with potential health benefits. Since knowledge about Thailand's various indigenous plants is limited, this study determined the nutritive values and bioactive compounds contained in eight edible indigenous plants growing within the conservation area of the Electricity Generating Authority of Thailand, Srinakarind Dam, Kanchanaburi province. Plant samples were analyzed as fresh or cooked (blanched or boiled) depending on customary preparation and consumption habits. Results showed that shoots and young leaves of Jang (Maerua siamensis Kurz), Ta-Kuk (Albizia lebbeck (L.) Benth), Pak-Wan-Pa (Melientha suavis Pierre), and Som-kob (Hymenodictyon exelsum Wall.) have potential health benefits in terms of nutritive values (vitamin C, dietary fiber, protein) and bioactive compounds (carotenoids, phenolic compounds, antioxidant activity). Shoots and young leaves of Jang were highest in protein, dietary fiber, phenolic compounds, and antioxidant activity. Shoots and young leaves of Ta-Kuk had the highest vitamin C level and considerable amounts of protein, dietary fiber, phenolic compounds, antioxidant activity, and carotenoids. Loss of vitamin C and bioactive compounds occurred most often in boiled plants rather than those that were blanched, though carotenoids increased with either boiling or blanching. This study's important findings should be translated into practical knowledge and disseminated to local communities and at the national level to encourage plant conservation, nutrition education, and the increased consumption of these indigenous plants.
Comparison of Biliary Complications Rates After Brain Death, Donation After Circulatory Death, and Living-Donor Liver Transplantation: A Single-Center Cohort Study
Authors: Meier Raphael Pascal Henri , Kelly Yvonne , Braun Hillary , Maluf Daniel , Freise Chris , Ascher Nancy , Roberts John , Roll Garrett
Published: December 2022

Donation-after-circulatory-death (DCD), donation-after-brain-death (DBD), and living-donation (LD) are the three possible options for liver transplantation (LT), each with unique benefits and complication rates. We aimed to compare DCD-, DBD-, and LD-LT-specific graft survival and biliary complications (BC). We collected data on 138 DCD-, 3,027 DBD- and 318 LD-LTs adult recipients from a single center and analyzed patient/graft survival. BC (leak and anastomotic/non-anastomotic stricture (AS/NAS)) were analyzed in a subset of 414 patients. One-/five-year graft survival were 88.6%/70.0% for DCD-LT, 92.6%/79.9% for DBD-LT, and, 91.7%/82.9% for LD-LT. DCD-LTs had a 1.7-/1.3-fold adjusted risk of losing their graft compared to DBD-LT and LD-LT, respectively (p < 0.010/0.403). Bile leaks were present in 10.1% (DCD-LTs), 7.2% (DBD-LTs), and 36.2% (LD-LTs) (ORs, DBD/LD vs. DCD: 0.7/4.2, p = 0.402/<0.001). AS developed in 28.3% DCD-LTs, 18.1% DBD-LTs, and 43.5% LD-LTs (ORs, DBD/LD vs. DCD: 0.5/1.8, p = 0.018/0.006). NAS was present in 15.2% DCD-LTs, 1.4% DBDs-LT, and 4.3% LD-LTs (ORs, DBD/LD vs. DCD: 0.1/0.3, p = 0.001/0.005). LTs w/o BC had better liver graft survival compared to any other groups with BC. DCD-LT and LD-LT had excellent graft survival despite significantly higher BC rates compared to DBD-LT. DCD-LT represents a valid alternative whose importance should increase further with machine/perfusion systems.
Algorithm for Determining the Types of Inverse Kinematics Solutions for Sequential Planar Robots and Their Representation in the Configuration Space
Authors: Chavdarov Ivan , Naydenov Bozhidar
Published: December 2022

The work defines in a new way the different types of solutions of the inverse kinematics (IK) problem for planar robots with a serial topology and presents an algorithm for solving it. The developed algorithm allows the finding of solutions for a wide range of robots by using a geometric approach, representing points in a polar coordinate system. Inverse kinematics, which is one of the most important, most studied and challenging problems in robotics, aims to calculate the values of the joint variables, given the desired position and orientation of the robot’s end effector. Configuration space is defined by joint angles and is the basis of most motion planning algorithms. Areas in the working and configuration space are generated that are reachable with different types of solutions. Programs are created that use the proposed algorithm for robots with two and three rotational degrees of freedom, and graphically present the results in the workspace and configuration space. The possibility of transitioning from one type of solution to another by passing through a singular configuration is discussed. The results are important for planning motions in the workspace and configuration space, as well as for the design and kinematic analysis of robots.
Influence of Ventilation Flow Rate and Gap Distance on the Radiative Heat Transfer in Aircraft Avionics Bays
Authors: Sanchez Florian , Liscouët-Hanke Susan , Bhise Tanmay
Published: December 2022

The feasibility of the future more-electric, hybrid-electric, and all-electric aircraft configurations will depend on a good understanding of thermal aspects early in the design. However, thermal analysis of aircraft equipment bays is typically performed at later design stages to validate if the design meets the minimal certification requirements rather than to optimize the cooling strategy. The presented work aims to provide new insight into thermal aspects in typical aircraft equipment bays. In particular, system thermal interactions, such as radiation, play a more significant role in tightly packaged bays, such as avionics bays. This paper investigates the influence of radiation on the overall system heat dissipation in two representative avionics bays. Using Computational Fluid Dynamics (CFD) simulation, combined with an analytical approach, the authors analyze the impact of several parameters, such as varying mass flow rates and distances between adjacent systems, on their thermal interaction. The results suggest that the radiative effects must be considered when the gap distance between the systems is larger than 0.1 m, the flow rate between two systems is not strong enough to have high convective heat exchanges, when the systems of interest are hidden by other systems from the ventilation sources, and when the system’s internal heat dissipation is significant. Overall, this paper’s results will contribute enhance conceptual design methods, such as the previously developed Thermal Risk Analysis, and help optimize thermal management strategies for future aircraft.
Advances, Implications, and Limitations of Low-Crude-Protein Diets in Pig Production
Authors: Rocha Gabriel Cipriano , Duarte Marcos Elias , Kim Sung Woo
Published: December 2022

Currently, five crystalline essential amino acids (Lys, Met, Thr, Trp, and Val) are generally used, allowing formulation of low-crude-protein (CP) diets. Moreover, Ile may also be used depending on its economic value and the specific feeding program. Experimentally, it has been shown that further reduced CP levels can be achieved by supplemental His, Leu, and Phe to the diets. However, decreasing the dietary CP level while maintaining optimal ratios of amino acids has shown contradictory effects on pigs’ growth performance. Due to the divergence in the literature and the importance for practical formulation strategies in the swine industry, a literature review and a meta-analysis were performed to estimate the minimum CP level that would not compromise pig performance. Based on the present review, there is a minimum CP level after which the growth performance of pigs can be compromised, even though diets are balanced for essential amino acids. Considering average daily gain and gain to feed, respectively, these levels were estimated to be 18.4% CP (95% confidence interval [CI]: 16.3 to 18.4) and 18.3% CP (95% CI: 17.4 to 19.2) for nursery, 16.1% CP (95% CI: 16.0 to 16.2) and 16.3% CP (95% CI: 14.5 to 18.0) for growing, and 11.6% CP (95% CI: 10.8 to 12.3) and 11.4% CP (95% CI: 10.3 to 12.5) for finishing pigs.
Alleviation of Catching and Crating Stress by Dietary Supplementation of Bacillus subtilis in Pekin Ducks
Authors: Mitin Helen , Zulkifli Idrus , Che Jamri Muhammad Hazim , Zamzuri Nur Athirah , Samian Nurain Aliah , Hussein Aimi Nabilah , Goh Yong Meng , Sazili Awis Qurni
Published: December 2022

Catching and crating may elicit stress and fear reactions in poultry because the procedures involve human contact and exposure to a novel environment. This study determined the effects of dietary probiotic supplementation on physiological stress, underlying fear, and growth performance of Pekin ducks subjected to catching and 4 h of crating. The study used a 2 × 2 factorial arrangement; the main factors were diet (basal or basal + probiotic) and crating durations (0 or 4 h). From 1 to 21 days of age (doa), birds were fed a basal or basal + probiotic (CLOSTAT® (Bacillus subtilis) (Kemin Industries, Inc., Des Moines, IA, USA), 1 g/kg) diet. At 21 doa, an equal number of ducklings from each dietary group were caught and crated for 4 h or left undisturbed in the home pens. Birds were examined for serum corticosterone (CORT), heat shock protein (HSP) 70, creatine kinase (CK), triglyceride (TG), glucose (GLU), cholesterol (CHOL), and lactate (LAC) concentrations, heterophil to lymphocyte ratios (HLR), tonic immobility (TI) duration, open-field (OF) test, body weight (BW), and feed conversion ratios (FCR). Diet had no significant (p > 0.05) effect on CORT among the non-crated ducks. However, after catching and crating, birds fed the control diet had significantly (p < 0.05) higher CORT than their probiotic-supplemented counterparts. Catching and crating significantly (p < 0.05) elevated HSP70, HLR, GLU, and CHOL but reduced TG in ducks. Birds fed the probiotic-supplemented diet showed significantly (p < 0.05) lower HSP70, HLR, TG, and CK than those fed the control diet. Probiotic-supplemented ducks showed reduced fear-related behaviours, including TI durations, ambulation latency, and body shaking. Diet had a negligible effect on body weights and FCR of ducks at 21 doa. In brief, catching and crating for 4 h augmented Pekin ducks’ physiological stress and fear reactions, and supplementing birds with probiotics was beneficial in ameliorating these detrimental effects.
A Grey Fuzzy Approach to the Selection of Cutting Process from the Aspect of Technological Parameters
Authors: Lukic Dejan , Cep Robert , Milosevic Mijodrag , Antic Aco , Zivkovic Aleksandar , Todic Vladimir , Rodic Dragan
Published: December 2022

This study deals with the selection of the cutting process using the grey fuzzy relation approach. The analysis was performed using plasma arc machining, laser beam machining, and abrasive waterjet machining on three different workpiece thicknesses with different cutting speeds. The objective was to select the best cutting process considering several performance characteristics such as machining time, dimensional accuracy, kerf width, and surface roughness. Data normalization, grey relation coefficients, fuzzy inference system, and grey fuzzy relation grade are used to evaluate the machining performances of the machining processes. The developed fuzzy model can be used to study the effects of different cutting processes on technological features. The results show that the grey fuzzy technique can be effectively used for the analysis and selection of cutting processes.
Understanding the Behavioural Intention to Play the Nintendo Switch: An Extension of the Technology Acceptance Model
Authors: Lin Chih-Wei , Lin Yu-Sheng , Xie Yi-Sheng , Chang Jui-Hsiu
Published: December 2022

Ever since the first games launched in the 1970s, the rapid growth of the video games market has successfully attracted more and more purchasers. In this study, we use Nintendo Switch as a case study to examine the extended technology acceptance model (TAM), which integrates the factors of Perceived Playfulness and Compatibility to determine their relevance in the usage behavior. A total of 266 questionnaires were obtained using snowball sampling and analyzed using the Structural Equation Modeling (SEM). The study results revealed that the expanded TAM model can be effectively used in this context and it also showed a good fit for the proposed model from the data analyzed. Attitude explained an R2 of 0.85, which indicates that 85% of the variance in user’s behavior intention is accounted by attitude. Perceived Ease of Use and Perceived Usefulness were significantly positively associated with attitude. Perceived Playfulness was significantly positively associated with Perceived Usefulness. This study also addresses the implications of the findings for researchers and the game marketing strategies practitioners.
Spatial Analysis of GHG Balances and Climate Change Mitigation in Rural Areas: The Case of Emilia–Romagna Region
Authors: Federico Katia , Bonora Alberto , Di Giustino Gianmarco , Reho Matelda , Lucertini Giulia
Published: December 2022

This paper aims to analyse the issue of mitigation and the balance of greenhouse gases in the rural contexts of the Emilia–Romagna region (Italy) due to climate change. The approach is based on the experimentation of a methodology, populated by available spatial databases and refined with a series of technical meetings, where it was possible to weigh availability and alternative choices within the identified assessment model. The objective of the research is to create a regional GHG balance map, in order to classify the territory for this specific dynamic. The aim of this approach is supporting policy decisions related to the Common Agricultural Policy at a regional level.
Myocardial Perfusion and Coronary Physiology Assessment of Microvascular Dysfunction in Patients Undergoing Transcatheter Aortic Valve Implantation—Rationale and Design
Authors: Dobrolinska M. M. , Gąsior P. , Błach A. , Gocoł R. , Hudziak D. , Wojakowski W.
Published: December 2022

The prevalence of coronary artery disease (CAD) in patients with severe aortic stenosis (AS) is 30–68%. Nevertheless, there is still not enough evidence to use invasive assessment of lesion severity, because the hemodynamic milieu of AS may impact the fractional flow reserve (FFR) and non-hyperemic indices. Therefore, the aim of the study is two-fold. First, to measure acute and long-term changes of FFR, index of microvascular resistance (IMR), and coronary flow reserve (CFR) in patients undergoing TAVI procedure. Second, to compare the diagnostic accuracy of intracoronary indices with myocardial perfusion measured by cadmium-zinc-telluride single-photon emission tomography (CZT-SPECT) and find cut-off values defining significant stenosis. We plan to enroll 40 patients eligible for TAVI with intermediate stenosis (30–70%) in the left anterior descending (LAD) coronary artery. In each patient FFR, CFR, and IMR will be measured in addition to myocardial blood flow calculated by CZT-SPECT before and either immediately after TAVI (acute cohort) or in 6 months (late cohort) after the procedure. FFR, CFR, and IMR will be matched with the results of myocardial perfusion measured by CZT-SPECT in the area of LAD. As a result, cut-off values of FFR, CFR, and IMR defining the decreased blood flow will be found.
EEG-Based Mapping of Resting-State Functional Brain Networks in Patients with Parkinson’s Disease
Authors: Leviashvili Sarah , Ezra Yael , Droby Amgad , Ding Hao , Groppa Sergiu , Mirelman Anat , Muthuraman Muthuraman , Maidan Inbal
Published: December 2022

(1) Background: Directed functional connectivity (DFC) alterations within brain networks are described using fMRI. EEG has been scarcely used. We aimed to explore changes in DFC in the sensory-motor network (SMN), ventral-attention network (VAN), dorsal-attention network (DAN), and central-executive network (CEN) using an EEG-based mapping between PD patients and healthy controls (HCs). (2) Methods: Four-minutes resting EEG was recorded from 29 PD patients and 28 HCs. Network’s hubs were defined using fMRI-based binary masks and their electrical activity was calculated using the LORETA. DFC between each network’s hub-pairs was calculated for theta, alpha and beta bands using temporal partial directed coherence (tPDC). (3) Results: tPDCs percent was lower in the CEN and DAN in PD patients compared to HCs, while no differences were observed in the SMN and VAN (group*network: F = 5.943, p < 0.001) in all bands (group*band: F = 0.914, p = 0.401). However, in the VAN, PD patients showed greater tPDCs strength compared to HCs (p < 0.001). (4) Conclusions: Our results demonstrated reduced connectivity in the CEN and DAN, and increased connectivity in the VAN in PD patients. These results indicate a complex pattern of DFC alteration within major brain networks, reflecting the co-occurrence of impairment and compensatory mechanisms processes taking place in PD.
Pulse Pressure Is Associated with Rapid Cognitive Decline over 4 Years: A Population-Based Cohort Study
Authors: Zhou Rong , Wei Shan , Wang Yanyu , Gao Ling , Dang Liangjun , Shang Suhang , Hu Ningwei , Peng Wei , Zhao Yi , Yuan Ye , Wang Jingyi , Wang Jin , Qu Qiumin
Published: December 2022

Aiming to investigate the relationship between pulse pressure (PP) and cognitive decline, cognitively normal subjects from a community-based longitudinal cohort were followed-up for 4 years. The Mini-Mental State Examination (MMSE) was used to evaluate global cognitive function, and a ≥2-point decrease in the MMSE score from baseline was defined as cognitive decline. Restricted cubic spline, multivariable linear regression and logistic regression were used to investigate the relationship between PP and cognitive decline. A total of 1173 participants completed the follow-up, and 205 (17.5%) met the criteria for cognitive decline. Restricted cubic splines showed no nonlinear relationship between PP and ΔMMSE (Poverall = 0.037, Pnon-linear = 0.289) or cognitive decline (Poverall = 0.003, Pnon-linear = 0.845). Multivariable linear regression analysis showed that PP was positively related to ΔMMSE (b = 0.021, p = 0.020). Multivariable logistic regression analysis showed that PP was positively associated with cognitive decline (OR = 1.020, p = 0.023). A stratified analysis found an association between PP and cognitive decline in participants who were aged ≤65 years, male, and APOEε4 noncarriers and who had school education ≤6 years or hypertension. A sensitivity analysis after propensity-score matching did not alter our findings. These findings highlight that elevated PP is associated with rapid cognitive decline, particularly in males, middle-aged, low-educated, hypertensive individuals and APOEε4 noncarriers.
The Intricate Interplay between the ZNF217 Oncogene and Epigenetic Processes Shapes Tumor Progression
Authors: Fahmé Pia , Ramadan Farah , Le Diep Tien , Thi Kieu-Oanh Nguyen , Ghayad Sandra E. , Hussein Nader , Diaz Chantal , Croset Martine , Clézardin Philippe , Cohen Pascale A.
Published: December 2022

The oncogenic transcription factor ZNF217 orchestrates several molecular signaling networks to reprogram integrated circuits governing hallmark capabilities within cancer cells. High levels of ZNF217 expression provide advantages to a specific subset of cancer cells to reprogram tumor progression, drug resistance and cancer cell plasticity. ZNF217 expression level, thus, provides a powerful biomarker of poor prognosis and a predictive biomarker for anticancer therapies. Cancer epigenetic mechanisms are well known to support the acquisition of hallmark characteristics during oncogenesis. However, the complex interactions between ZNF217 and epigenetic processes have been poorly appreciated. Deregulated DNA methylation status at ZNF217 locus or an intricate cross-talk between ZNF217 and noncoding RNA networks could explain aberrant ZNF217 expression levels in a cancer cell context. On the other hand, the ZNF217 protein controls gene expression signatures and molecular signaling for tumor progression by tuning DNA methylation status at key promoters by interfering with noncoding RNAs or by refining the epitranscriptome. Altogether, this review focuses on the recent advances in the understanding of ZNF217 collaboration with epigenetics processes to orchestrate oncogenesis. We also discuss the exciting burgeoning translational medicine and candidate therapeutic strategies emerging from those recent findings connecting ZNF217 to epigenetic deregulation in cancer.
GMEB2 Promotes the Growth of Colorectal Cancer by Activating ADRM1 Transcription and NF-κB Signalling and Is Positively Regulated by the m6A Reader YTHDF1
Authors: Ning Zhengping , Wu Zhiwei , Zhang Fan , Yang Ming , Lu Zhixing , Yu Bowen , Long Fei , Guo Yihang , Yang Kaiyan , Hu Gui , Zhang Yi , Li Xiaorong , Li Liang , Lin Changwei
Published: December 2022

Transcription factors are frequently aberrantly reactivated in various cancers, including colorectal cancer (CRC). However, as a transcription factor, the role of GMEB2 in cancer is still unclear, and further studies are needed. Here, we aimed to identify the function and mechanism of GMEB2 in regulating the malignant progression of CRC. GMEB2 was found to be highly expressed in online data analyses. We demonstrated that GMEB2 was markedly upregulated at both the mRNA and protein levels in CRC cells and tissues. GMEB2 knockdown inhibited CRC cell growth in vitro and in vivo. Mechanistically, as a transcription factor, GMEB2 transactivated the ADRM1 promoter to increase its transcription. Rescue experiments showed that ADRM1 downregulation partially reversed the promoting effects of GMEB2 on CRC growth in vitro. Moreover, the GMEB2/ADRM1 axis induced nuclear translocation of NF-κB, thus activating NF-κB signalling. Finally, we further revealed that YTHDF1 recognized and bound to the m6A site on GMEB2 mRNA, which enhanced its stability. Taken together, our findings reveal the crucial role and regulatory mechanism of GMEB2 in CRC for the first time and provide a novel potential therapeutic target for CRC therapy.
An Evaluation of the Anticancer Properties of SYA014, a Homopiperazine-Oxime Analog of Haloperidol in Triple Negative Breast Cancer Cells
Authors: Asong Gladys M. , Voshavar Chandrashekhar , Amissah Felix , Bricker Barbara , Lamango Nazarius S. , Ablordeppey Seth Y.
Published: December 2022

Triple negative breast cancer (TNBC) is a type of breast cancer associated with early metastasis, poor prognosis, high relapse rates, and mortality. Previously, we demonstrated that SYA013, a selective σ2RL, could inhibit cell proliferation, suppress migration, reduce invasion, and induce mitochondria-mediated apoptosis in MDA-MB-231 cell lines, although we were unable to demonstrate the direct involvement of sigma receptors. This study aimed to determine the anticancer properties and mechanisms of action of SYA014, [4-(4-(4-chlorophenyl)-1,4-diazepan-1-yl)-1-(4-fluorophenyl)butan-1-one oxime], an oxime analogue of SYA013, the contribution of its sigma-2 receptor (σ2R) binding, and its possible synergistic use with cisplatin to improve anticancer properties in two TNBC cell lines, MDA-MB-231 (Caucasian) and MDA-MB-468 (Black). In the present investigation, we have shown that SYA014 displays anticancer properties against cell proliferation, survival, metastasis and apoptosis in the two TNBC cell lines. Furthermore, a mechanistic investigation was conducted to identify the apoptotic pathway by which SYA014 induces cell death in MDA-MB-231 cells. Since SYA014 has a higher binding affinity for σ2R compared to σ1R, we tested the role of σ2R on the antiproliferative property of SYA014 with a σ2R blockade. We also attempted to evaluate the combination effect of SYA014 with cisplatin in TNBC cells.
Shedding a Light on the Challenges of Adolescents and Young Adults with Rhabdomyosarcoma
Authors: Ferrari Andrea , Gatz Susanne Andrea , Minard-Colin Veronique , Alaggio Rita , Hovsepyan Shushan , Orbach Daniel , Gasparini Patrizia , Defachelles Anne-Sophie , Casanova Michela , Milano Giuseppe Maria , Chisholm Julia C. , Jenney Meriel , Bisogno Gianni , Rogers Timothy , Mandeville Henry C. , Shipley Janet , Miah Aisha B. , Merks Johannes H. M. , van der Graaf Winette T. A.
Published: December 2022

Rhabdomyosarcoma (RMS) is a typical tumour of childhood but can occur at any age. Several studies have reported that adolescent and young adult (AYA) patients with RMS have poorer survival than do younger patients. This review discusses the specific challenges in AYA patients with pediatric-type RMS, exploring possible underlying factors which may influence different outcomes. Reasons for AYA survival gap are likely multifactorial, and might be related to differences in tumor biology and intrinsic aggressiveness, or differences in clinical management (that could include patient referral patterns, time to diagnosis, enrolment into clinical trials, the adequacy and intensity of treatment), as well as patient factors (including physiology and comorbidity that may influence treatment tolerability, drug pharmacokinetics and efficacy). However, improved survival has been reported in the most recent studies for AYA patients treated on pediatric RMS protocols. Different strategies may help to further improve outcome, such as supporting trans-age academic societies and national/international collaborations; developing specific clinical trials without upper age limit; defining integrated and comprehensive approach to AYA patients, including the genomic aspects; establishing multidisciplinary tumor boards with involvement of both pediatric and adult oncologists to discuss all pediatric-type RMS patients; developing dedicated projects with specific treatment recommendations and registry/database.
Comparative Analysis of mRNA and miRNA Expression between Dermal Papilla Cells and Hair Matrix Cells of Hair Follicles in Yak
Authors: Zhang Xiaolan , Bao Pengjia , Zheng Qingbo , Chu Min , Liang Chunnian , Guo Xian , Wu Xiaoyun , He Meilan , Pei Chengfang , Yan Ping
Published: December 2022

The interaction between the dermal papilla cells (DPCs) and epidermal hair matrix cells (HMCs) of hair follicles (HFs) is crucial for the growth and development of HFs, but the molecular mechanism is complex and remains unclear. MicroRNAs (miRNAs) are the key signaling molecules for cellular communication. In this study, the DPCs and HMCs of yak were isolated and cultured, and the differentially expressed mRNA and miRNA were characterized to analyze the molecular basis of the interaction between DPCs and HMCs during hair follicle (HF) development in yak. The mRNA differential expression and functional enrichment analysis revealed that there were significant differences between DPCs and HMCs, and they showed the molecular functional characteristics of dermal cells and epidermal cells, respectively. Multiple KEGG pathways related to HF development were enriched in the highly expressed genes in DPCs, while the pathways associated with microbiota and immunity were significantly enriched in the highly expressed genes in HMCs. By combining analysis with our previous 10× genomics single-cell transcriptome data, 39 marker genes of DPCs of yak were identified. A total of 123 relatively specifically expressed miRNAs were screened; among these, the miRNAs associated with HF development such as miR-143, miR-214, miR-125b, miR-31, and miR-200 were presented. In conclusion, the large changes in yak DPCs and HMCs for both mRNA and miRNA expression were revealed, and numerous specifically expressed mRNAs and miRNAs in DPCs or HMCs were identified, which may contribute to the interaction and cellular communication between DPCs and HMCs during HF development in yak.
Protective Properties of Thermal-Insulation Coatings under Conditions of Long-Term Exposure to Natural Climatic Factors
Authors: Zhumadilova Zhanar O. , Nurlybayev Ruslan E. , Orynbekov Yelzhan S. , Joldassov Aidos A. , Abdikarimov Malik N.
Published: December 2022

The purpose of this study was to analyze the stability of the protective properties of thermal-insulation coatings under long-term exposure to natural climatic factors. An analysis of the changes in the decorative characteristics was carried out using a CD-6834 spectro-guide sphere gloss spectrophotometer; for the mechanical tensile testing of the polymer composites, an AGS-X series tensile testing machine, TRAPEZIUM X software, and a PSO MG4 device were used to determine the adhesion strength of facing and protective coatings. The results showed that in most cases, only full-scale climatic tests to determine the qualitative indicators of epoxy coatings (ECs), such as indicators of the viability and exothermicity of epoxy compositions, changes in the viscosity of epoxy binders, and the tensile strength and thermal conductivity, allowed us to evaluate the effects of changing the epoxy coating properties in full-scale conditions. When analyzing changes in the characteristics of the polymer samples after exposure to climatic factors, it was found that the compositions based on epoxy resin ED-20, modified epoxy resin Etal-247, active thinner Etal-1, and hardener Etal-45M demonstrated the best elastic and strength characteristics.
Polycrystalline Silicon Thin Films for Solar Cells via Metal-Induced Layer Exchange Crystallization
Authors: Zamchiy Alexandr O. , Baranov Evgeniy A.
Published: December 2022

Solar photovoltaics (PV) has the potential to take center stage in global energy in the future [...]
Evaluation of the Effect of Various Cementation Protocols Used for 10% Zirconia-Reinforced Lithium Glass Ceramic Veneer on Shear Bond Strength to Resin Cement (An In Vitro Study)
Authors: Al-Zahawi Abdulsalam Rasheed
Published: December 2022

Bonding failure between ceramic restoration and cement dramatically influences the success of resin-bonded ceramic restoration. This study evaluates the influence of various fitting surface treatments of 10% zirconia-reinforced lithium glass ceramic (ZLS) on its shear bonding strength to resin cement. Sixty blocks sized 8 × 8 × 2 mm3 were cut from a ZLS. All specimens were fired for 10 min and separated into six groups according to surface treatment: GI (Without treatment), GII (10% HF acid, Ultra Sound water bath US, and silane coupling agent S), GIII (HF, US, S, and bonding without light cure B1), GIV (HF, US, 37% phosphoric acid PA, US, S, B1), GV (HF, US, S, and bonding with light curing B2), VI (HF, US, PA, US, S, and B2). The treated specimens were cemented to a resin cement cylinder of 3.5 mm in diameter and 2 mm in height (Variolink, Esthetic. Neutral). A universal test machine was used to test the shear bond strength (SBS) and SEM for failure mode. The result indicated that applying a bonding agent on the prepared surface of ZLS without curing before cementation significantly increases the shear bond strength and affects the failure mode. In contrast, the application of PA does not influence bond strength.
Probing the Role of a Conserved Phenylalanine in the Active Site of Thiocyanate Dehydrogenase
Authors: Varfolomeeva Larisa A. , Solovieva Anastasia Yu. , Shipkov Nikolai S. , Kulikova Olga G. , Dergousova Natalia I. , Rakitina Tatiana V. , Boyko Konstantin M. , Tikhonova Tamara V. , Popov Vladimir O.
Published: December 2022

Copper-containing enzymes catalyze a broad spectrum of redox reactions. Thiocyanate dehydrogenase (TcDH) from Thioalkalivibrio paradoxus Arh1 enables the bacterium to use thiocyanate as a unique source of energy and nitrogen. Oxidation of thiocyanate takes place in the trinuclear copper center of TcDH with peculiar organization. Despite the TcDH crystal structure being established, a role of some residues in the enzyme active site has yet to be obscured. F436 residue is located in the enzyme active site and conserved among a number of TcDH homologs, however, its role in the copper center formation or the catalytic process is still not clear. To address this question, a mutant form of the enzyme with F436Q substitution (TcDHF436Q) was obtained, biochemically characterized, and its crystal structure was determined. The TcDHF436Q had an unaltered protein fold but did not possess enzymatic activity, whereas it contained all three copper ions, according to ICP-MS data. The structural data showed that the F436Q substitution resulted in a disturbance of hydrophobic interactions within the active site crucial for a correct transition between open/closed forms of the enzyme–substrate channel. Thus, we demonstrated that F436 does not participate in copper ion binding, but rather possesses a structural role in the TcDH active site.
Termination Control of (001) and (110) NdGaO3 Single-Crystal Substrates by Selective Chemical Etching
Authors: Leca Victor
Published: December 2022

Controlling the surface morphology and composition of the perovskite substrates is a critical aspect in tuning the final properties of the deposited films and of their interfaces. The paper reports on a chemical etching method developed for (110) and (001) NdGaO3 single crystal substrates in order to obtain a well-defined GaO2−x-terminated surface. The etching process is based on a HF + NH4OH solution and includes an annealing step performed in air or under O2 flow at temperatures of 800–1000 °C. In order to obtain the desired composition and surface morphology, the etching procedure was optimized for the vicinal step density at the surface and substrate crystal orientation. Growth nucleation studies of one-unit-cell MeO films (Me = Ti, Sr, Ba) on chemically etched and on only annealed substrates were performed in order to determine the composition of the substrate topmost layer. The results indicate that the chemically etched NdGaO3 substrate surface has a predominantly GaO2−x termination, with a lower free surface energy compared to the NdO1+x termination.
Morphological and Molecular Characterizations of Three Species of the Genus Synura (Synurales, Chrysophyceae) from China
Authors: Hao Junxue , Nan Fangru , Lv Junping , Liu Qi , Liu Xudong , Xie Shulian , Feng Jia
Published: December 2022

Three species of the genus Synura from China are described here. Morphological observations and molecular phylogenetic analyses were conducted for three specimens collected at different locations in China. The described morphological features included cell size, scale size, spines, keels, and struts. Molecular analyses based on multiple genetic markers (SSU and LSU rDNA and internal transcribed spacer rDNA) were used to determine the phylogenetic positions of the three Synura species. Morphologically, specimen GZ201017 collected in Guizhou Province was characterized by a well-developed keel and lanceolate scales; specimen SX210304 collected in Shanxi Province was characterized by a less-developed keel and poor silicification; and specimen GD201126 collected in Guangdong Province was characterized mainly by spines with blunt ends or two small teeth on the tips. The morphotypes GZ201017, SX210304, and GD201126 corresponded to the original descriptions of Synura petersenii, S. glabra, and S. longitubularis, respectively. This discovery laid a foundation for the molecular phylogeny of the genus Synura and an enhanced understanding of Synura diversity and distribution in China.
HDGFI: Hierarchical Dual-Level Graph Feature Interaction Model for Personalized Recommendation
Authors: Ma Xinxin , Cui Zhendong
Published: December 2022

Under the background of information overload, the recommendation system has attracted wide attention as one of the most important means for this problem. Feature interaction considers not only the impact of each feature but also the combination of two or more features, which has become an important research field in recommendation systems. There are two essential problems in current feature interaction research. One is that not all feature interactions can generate positive gains, and some may lead to an increase in noise. The other is that the process of feature interactions is implicit and uninterpretable. In this paper, a Hierarchical Dual-level Graph Feature Interaction (HDGFI) model is proposed to solve these problems in the recommendation system. The model regards features as nodes and edges as interactions between features in the graph structure. Interaction noise is filtered by beneficial interaction selection based on a hierarchical edge selection module. At the same time, the importance of interaction between nodes is modeled in two perspectives in order to learn the representation of feature nodes at a finer granularity. Experimental results show that the proposed HDGFI model has higher accuracy than the existing models.