bluuebunny/crossref_metadata_2025 huggingface dataset:
Pemberdayaan Masyarakat Pesisir Melalui Produksi Bunga Jantung Pisang Crispy di Dusun Upunyor
Authors: Sugiarto Sigit , Kamaruddin Sitti Fatimah , Kurniati MA Ratnah , Lestari Lestari , Leunupun Engrith Grafelia , Alam Asmirani , Rarutioka Krismendo , Monjel Astin , Romrainy Salomina , Kastera Maljhon , Aitiawisima Rahel
Published: November 2023

Program pembuatan bunga jantung pisang crispy ini bertujuan untuk memberikan kesadaran dan pengetahuan kepada masyarakat Dusun Upunyor mengenai cara pengolahan jantung pisang sehingga memiliki nilai jual yang tinggi. Hal ini untuk menjawab tantangan kurang optimalnya pemanfaatan jantung pisang yang merupakan hasil pertanian masyarakat Dusun Upunyor. Program pembuatan bunga jantung pisang crispy ini berupa demonstrasi pegolahan, pengenalan kemasan, hingga pemasaran produk. Produk yang dihasilkan dari program ini banyak diminati oleh konsumen dan memiliki nilai jual tinggi. Mayoritas konsumen merasa sangat puas terhadap produk yang dihasilkan baik dari segi rasa, tampilan kemasan, maupun harga. Setelah pelaksanaan program, masyarakat tidak hanya mengolah jantung pisang menjadi sayur, tapi juga menjadi makanan ringan berupa bunga jantung pisang crispy yang memiliki nilai jual tinggi. Sehingga hasil produksi pertanian berupa jantung pisang dari masyarakat Dusun Upunyor dapat dimanfaatkan dengan lebih optimal
AI in Auditing: A Comprehensive Review of Applications, Benefits and Challenges
Authors:   Southern India Regional Council of the Institute of Chartered Accountants of India (SIRC of ICAI),   Chennai, Tamil Nadu, India, Ganapathy Venkatasubramanian
Published: November 2023

The rapid evolution of artificial intelligence (AI) technologies has brought about transformative changes in various industries, and the field of auditing is no exception. This research paper presents a comprehensive review of the integration of AI in auditing practices, highlighting its applications, benefits, and associated challenges. Auditing, a critical process for ensuring the accuracy and reliability of financial information, has traditionally been a labor-intensive and time-consuming endeavor. The emergence of AI technologies, such as machine learning, natural language processing, and data analytics, has revolutionized the way audits are conducted. AI-powered auditing tools offer advanced capabilities for data analysis, pattern recognition, anomaly detection, and risk assessment. These capabilities enhance the effectiveness and efficiency of audits by allowing auditors to focus on high-risk areas and perform more in-depth analysis. The paper explores various applications of AI in auditing, including: Automated Data Analysis, Predictive Analytics, Fraud Detection, and Natural Language Processing (NLP), Continuous Monitoring. While AI brings significant benefits to the auditing process, its adoption also presents certain challenges like Data Quality and Integration, Interpretability, Ethical Considerations, Technical Expertise, Regulatory Frame Work. In conclusion, AI has the potential to revolutionize auditing practices by enhancing efficiency, accuracy, and risk assessment. However, successful integration requires addressing challenges related to data quality, transparency, ethics, skills, and regulations. As AI technologies continue to evolve, auditors and stakeholders must collaborate to harness the full potential of AI while maintaining the integrity and credibility of the auditing process. This paper serves as a comprehensive resource for auditors, researchers, and policymakers seeking to understand the current landscape and future directions of AI in auditing.
Exploring the Intersections of Community and Cross-Cultural Psychology: Enhancing Well-being and Understanding Diversity
Authors:   Dean, Faculty of Education, Kalinga University, Naya Raipur. (C.G), Verma Shraddha
Published: November 2023

This abstract delves into the dynamic interplay between community psychology and cross-cultural psychology, highlighting their roles in promoting psychological well-being, addressing social issues, and fostering a deeper understanding of cultural diversity. Community psychology focuses on the reciprocal relationship between individuals and their communities, emphasizing the importance of context in shaping human behavior and well-being. By employing an ecological perspective, community psychologists strive to empower communities, prevent social problems, and advocate for social justice. This approach recognizes the impact of various factors, including economic disparities, social support networks, and neighborhood environments, on individual psychological experiences. Cross-cultural psychology, on the other hand, investigates the intricate connections between culture and psychology. It seeks to uncover both universal and culturally specific aspects of human behavior, cognition, and emotion. By comparing psychological phenomena across cultures, cross-cultural psychologists illuminate the diverse ways in which cultural norms, values, and traditions influence individuals’ thoughts and actions. This field plays a crucial role in challenging ethnocentric biases and enriching our understanding of the human experience. The abstract highlights the convergence of these two fields, emphasizing how they mutually enrich one another. Community psychology benefits from the insights of cross-cultural psychology by recognizing the importance of cultural context in community dynamics and interventions. Cross-cultural psychology gains depth by integrating the community perspective, recognizing that culture is not solely an individual attribute, but a collective phenomenon shaped by the communities in which people reside. Through collaborative research, interventions, and advocacy efforts, these fields contribute to building more inclusive and equitable societies. By acknowledging the importance of community and cultural influences on psychological well-being, researchers and practitioners can better address societal challenges, bridge gaps in mental health care, and create interventions that are sensitive to the diverse needs of individuals from various backgrounds. In conclusion, the synergy between community psychology and cross-cultural psychology holds great potential for advancing our understanding of human behavior and well-being within the contexts of communities and cultural diversity. This abstract encourages continued exploration and integration of these fields to create meaningful impact and positive change in diverse societies around the world.
Grihastha Ashrama And The Kamasutra
Authors:   Assistant Professor, Department of Philosophy, Jai Hind College (Autonomous), Mumbai, Bawa Simmin
Published: November 2023

The Kamasutra is an ancient text guiding the sensual and sexual life of men and women in society. The text authored by Mallinga Vatsyayana is a work of art describing the variety of human sexual conduct and its pursuit from the time the person is in the Grihastha ashrama or the householder’s stage of existence. This included men, women and the third gender. The Grihastha stage begins from the time studentship stage of Brahmacharya Ashrama ends and the person enters the stage of a householder where the person begins a professional career, acquires sources of income, gets married and begets children. The main protagonist of the Kamasutra, the “nagaraka” after acquiring education, sets up a lifestyle for himself, follows duties, participates in festivities, attends social gatherings, etc. The nagaraka also has a set of social skills and etiquettes to follow; maintain a wardrobe full of various attires for each function and makes efforts to maintain personal grooming; hosting and entertaining family, friends, lovers, courtesans and acquaintances, conduct business trade and maintain networking in social circles as well as indulge in cultural pursuits. Women had to focus on grooming, acquiring skills, maintaining the household and balancing between various relationships. But nowhere has Vatsyayana focused on mentioning stages of life where one procreates or prepares for Vanaprastha Ashrama or Sanyasa Ashrama or pursuit of Moksha or liberation. These aspects of the life of a commoner need to be explored at greater lengths in the academic arena for a holistic study on the great monumental work of Vatsyayana as Kamasutra is only seen as a work on sexual positions and portrayals. The current study proposes to explore various facets of the Grihastha life spelled out explicitly and implicitly by the author and aspects missed or avoided by him in the greater good of life. As we spend a large part of our life as householders, the views expressed by Vatsyayana hold good and true in these contemporary times as well which needs to be investigated exhaustively.
A kivívott és közben látenssé vált állampolgári jog. (Az angol esküdtszék története – Antal Tamás)
Authors: Rigó Balázs
Published: November 2023

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Performance of broiler chickens fed compound feeds with various of cereal grains
Authors:   Agricultural Academy, Agricultural Institute - Stara Zagora, 6000, Bulgaria, Hristakieva Pavlina , Ivanova Ivelina ,   Agricultural Academy, Agricultural Institute - Stara Zagora, 6000, Bulgaria, Mincheva Nadya ,   Agricultural Academy, Agricultural Institute - Stara Zagora, 6000, Bulgaria, Mihaylova Milena ,   Agricultural Academy, Agricultural Institute - Stara Zagora, 6000, Bulgaria, Oblakova Magdalena ,   Agricultural Academy, Agricultural Institute - Stara Zagora, 6000, Bulgaria
Published: November 2023

Maize and wheat are the main cereal forage crops cultivated at a global scale. They are among preferred forage grains for poultry nutrition. The aim of this study was to evaluate the productive performance of broiler chickens, whose compound feeds contained either maize, or wheat alone and maize +wheat combination. The experiment was performed with 120 day-old male ROSS hybrid chicks reared on the floor for 49 days. The birds were divided into three groups, each one consisting of two subgroups, as follows: group Е1 – fed forage with maize and wheat; group Е2 – fed forage with wheat and group Е3 – forage with maize. The live weight, feed intake, feed conversion and slaughter traits were investigated. By the 49th day of age, the live weight of chickens from groups Е1 and Е2 fed diet with maize and wheat (35.99%+28%), and only wheat without maize (66%) were the heaviest, 3147.18 g and 3361.32 g, respectively. Chicks from group E3 fed the diet containing only maize (64.12%) without wheat had the lowest average live weight (2587.75 g at Р<0.0001). Wheat, as a single cereal in broiler compound feeds, and its combination with maize had the most beneficial effect on weight gain and feed conversion over the entire fattening period (P<0.001). The improved weight gain of birds fed these diets was accompanied with higher feed intake (P<0.001) compared to birds fed forage with maize only. The slaughter yield and the breast with bone cut increased statistically significantly in the wheat-fed groups (P<0.001).
Influence of factors on change in gross profit and level of profitability in milk production from Bulgarian Dairy Synthetic Population
Authors:   Agricultural Academy, Institute of Animal Sciences - Kostinbrod, Mihailova-Toneva Mariya , Ivanova Tanya ,   Agricultural Academy, Institute of Animal Sciences - Kostinbrod
Published: November 2023

The purpose of the present study is an analysis of the influence of various factors on the indicators of gross profit and level of profitability in the production of milk from sheep of the Bulgarian Dairy Synthetic Population breed for a period of 3 years. Data from the accounting department of Institute of Animal Sciences -Kostinbrod were used. The indicators were analyzed: average yield (l), average selling price of 1 l of milk, cost of variable production costs for 1 l of milk, gross income from milk, gross profit, level of profitability of variable costs. The index factor analysis (method of chain substitution) was used to determine the influence of each factor on the value of the outcome indicator. The absolute change in income (by 31.79%) over the three-year period has an increasing trend. Variable costs are reduced and this has a positive impact on gross profit. In the third year, the reduction in feed cost increases the gross profit. The contribution margin per 1 l of milk has an increasing effect on the level of profitability (by 39.78% and 56.1%) for the second and third years. The level of profitability increased in the third year by 55.65%.
The three lines model and role of internal audit is listed companies
Authors:   HCMC University of Economics and Finance, Le Dung Phuong
Published: November 2023

Internal audit is an important part of corporate governance. Internal Audit as an independent and objective supervisor of operating processes, giving opinions to improve the efficiency of risk management processes, control processes and governance processes. Thereby, providing the Board of Directors and leaders with an independent view to promote cooperation, monitoring and early warning about new risks, improving the efficiency of business processes to add value and improve operations, while playing a catalytic role and fostering an internal corporate culture of risk management and compliance. In this article, the author mentions the three lines model and compares the three lines model with the previous three lines model of defense to identify challenges internal audit in joint stock companies. The research results make recommendations to enhance the role of internal audit in governance in order to minimize risks, fraud, and bring more value to businesses at Joint stock companies.
Effects of capital structure on performance of commercial banks in Vietnam
Authors:   HCMC Open University, Nguyen Diep Van , Bui Thanh Dan ,   HCMC Banking University
Published: November 2023

This paper adds empirical evidence on the relationship between capital structure and the performance of commercial banks. We use balance sheet data of 25 joint stock commercial banks operating in Vietnam from 2011 to 2021. ROE measures the performance, and the total debt ratio is the explanatory variable. Regression techniques REM, FEM, and FGLS were used to explore this relationship. The results show that there is no evidence to confirm the impact of capital structure on the performance of banks. Also, the paper shows the positive effects of the control variables on the performance of joint stock commercial banks in Vietnam.
Factors influencing the Dynamic Competitive Capability of 4-5 star Vietnamese brand hotels in Khanh Hoa Province
Authors:   Khanh Hoa University, Phan Yen Hai Thi , Nguyen Thang Quyet ,   HUTECH University
Published: November 2023

Research on factors influencing the Dynamic Competitive Capability of 4-5 star Vietnamese brand hotels in Khanh Hoa. The sample size for analysis consists of 250 surveys using SPSS 22 and Amos 22 software. The results indicate that all six proposed factors positively influence the dynamic competitive capability of Vietnamese brand hotels in Khanh Hoa, with decreasing levels of impact as follows: Business orientation capability, Creativity capability, Organizational capability, Application of Science and Technology capability, Marketing capability, and Learning Orientation capability. Based on these findings, the author proposes several implications to enhance the dynamic competitive capability of Vietnamese brand hotels in the future.
The ability to access credit capital of small and medium enterprises in Binh Duong Province
Authors:   Thu Dau Mot University, Nguyen Thu Hong , Nguyen Linh Xuan Tran ,   HCMC University of Finance - Marketing
Published: November 2023

The study evaluated several factors affecting the ability to access credit capital of small and medium enterprises in Binh Duong province. Through survey data from 450 enterprises, the authors analyzed several factors, including enterprise capacity, social relations of enterprises, business plans, financial statements of enterprises, institutional quality, ability to adapt to risks of enterprises, banks, and credit institutions, and entrust loan organization. The results show that, in addition to businesses needing to improve their internal capabilities, they need to improve the support from banks, credit institutions, and entrust loan organizations that could help enterprises to access formal credit.
Enhancing the linkage between the Foreign Trade University and Industries with the Japanese model “Industry - University - Government”
Authors:   Hanoi University, Trinh Anh Hoai , Bui Tuan Anh ,   Foreign Trade University, Tran Loc Xuan ,   Foreign Trade University
Published: November 2023

Linkage between universities and industries play an important role in improving the quality of high-quality human resource training as well as enhacing research and knowledge transfer to industries. Foreign Trade University is one of the universities in Viet Nam pioneering in building and developing linkage between universities and industries with many practical models. The model “Industry - University - Government” is a popular model in research and practical implementation of linkages between universities and industries, in which the state plays an important role (central and local). On the basis of the “Industry - University - Government “ model approach, the author analyzes and evaluates the linkage between Foreign Trade University and Industries and offers solutions and recommendations to further strengthen the colloboration. The fact that the linkage between Foreign Trade University and Industries is a good lesson for many universities and industries in Viet Nam.
NKF Peers Mentoring Program
Authors: Collins, MSW, LICSW Kelli
Published: November 2023

The article provides an overview of the National Kidney Foundation’s Peers mentoring program. Following a brief discussion of the evidence behind peer support across disease states, the article reviews data collected since the program’s official launch in September 2011 through December 31, 2015. Potential challenges and lessons learned from the program to date are highlighted and discussed.
Saved and Missing CMS-2728 Forms Could Affect ESRD Patients’ Medicare Enrollment Benefits
Authors: Delva, BA, CTT+ Oniel
Published: November 2023

Since 2012, healthcare professionals at Medicare-certified dialysis facilities have used CROWNWeb to electronically submit CMS-2728 Medicare Entitlement and/or Patient Registration forms directly to the Centers for Medicare & Medicaid Services (CMS). While a vast majority of CMS-2728 forms continue to be submitted to CMS and the Social Security Administration (SSA) correctly, data reveal an increase in the number of incomplete forms between 2012 and 2016. This article discusses the reasons forms remain incomplete in CROWNWeb, identifies how users can work to complete forms within a timely manner, and provides an overview of CMS’s 2017 CMS-2728 form submission goals.
The Role for Nephrology Social Work in the New Kidney Disease Paradigm—Moving Ahead by Remembering How We Got Here
Authors: Browne, PhD, MSW Teri
Published: November 2023

2019 brings exciting changes to the delivery of kidney disease care in the United States and beyond. We have increasing attention to home dialysis and kidney transplantation as preferred treatment modalities for end-stage renal disease (ESRD), new organizations such as CVS Pharmacy and Cricket expanding leadership in chronic kidney disease (CKD) treatment, innovative treatment options such as wearable and implantable kidneys on the horizon, and a new stream of funding for kidney disease innovation throughKidneyX. These changes are happening at the same time that patient-centered research is becoming the standard in outcomes evaluation. Nephrology social workers (NSWs) must act now to position ourselves as leaders in this new kidney disease paradigm; these opportunities present an exciting time for social workers to highlight how our interventions can help patients have the best outcomes as kidney disease care evolves.
Research on Aircraft image recognition based on transfer learning and improved YOLOv5 model
Authors: Yang Huanyu , Yang Lijun , Bo Yuming , Wang Jun
Published: November 2023

Effective differentiation of aircraft types using images is important for providing military combat information as well as civilian aircraft operations. Aircraft image recognition has many difficulties such as large variations of target scale, complex backgrounds, and difficult data set acquisition, which lead to the low recognition accuracy of existing models. To address the problem of low recognition accuracy caused by the above difficulties. This paper proposes the improved YOLOv5 model for the recognition of aircraft images. First, this paper designs the CSPResNet50dCA network as the backbone of the YOLOv5 model to enhance the feature extraction capability for small target aircraft in images. By introducing the coordinate attention mechanism and the CSP structure, the feature-focusing capability and the computing speed of the model are enhanced. Afterward, we use data enhancement to expand the data set and transfer learning to improve the generalization ability and convergence speed of the model, so as to improve its robustness. The experimental results show that the improved YOLOv5 model has significantly improved the recognition accuracy of aircraft targets， and significantly enhanced feature extraction ability for small target aircraft with good generalization ability.
PENENTUAN CACAT DENGAN METODE ULTRASONIC TESTING
Authors: Supriyanto Adolf Asih
Published: November 2023

Dalam penelitian ini telah dilakukan penentuan cacat suatu bahan menggunakan metode ultrasonic testing. Sebelum menentukan cacat bahan, terlebih dahulu menentukan titik indeks dan kalibrasi jarak Probe Sudut. Kalibrasi jarak Probe Sudut dilakukan dengan mengatur velocity = 3250 m/s, range = 100 mm, sudut = 60o, posisi low = 25 mm dan posisi high = 100 mm, masing- masing untuk jarak r sama dengan 25, 50 dan 100 mm. Perlatan yang digunakan dalam penelitian ini adalah Ultrasonic Testing Raptor, Probe Sudut, Bahan Uji standar V1 dan V2, kuplan, pengggaris, kertas dan kalkulator. Ukuran benda uji adalah 260×75×50 mm3. Hasil yang didapat menunjukkan bahwa pada bahan telah ditemukan tiga buah cacat masing-masing pada kedalaman 37,19 mm dengan panjang 30 mm, kedalaman 11,87 mm dengan panjang 45 mm dan kedalaman 23,71 mm dengan panjang 38 mm.
SISTEM KONTROL KECEPATAN MOTOR UNIVERSAL MENGGUNAKAN PID ARDUINO
Authors: Setiawan Iwan , Agung Nugroho Emmanuel , Roni Wibowo Nanang , Janizal
Published: November 2023

Motor universal telah banyak sekali digunakan pada peralatan rumahtangga, diantaranya mixer, mesin bor listrik, mesin gerinda, mesin jahit dan banyak lagi penggunaan lainnya. Motor universal merupakan motor yang dapat beroperasi dengan menggunakan sumber tegangan AC maupun DC dengan karakteristik khas-nya adalah kecepatan putar yang tinggi hingga di atas 3200 rpm. Kecepatan putar motor universal dapat dikendalikan dengan menggunakan thyristor yang menghasilkan tegangan DC ataupun tegangan AC terkendali. Pengaturan tegangan AC atau DC terkendali pada thyristor dilakukan dengan memberikan pulsa pemicuan pada gate thyristor. Untuk mendapatkan kecepatan putaran yang diharapkan digunakan sistem kontrol dengan pengendali PID. Sistem Kontrol PID (Proportional–Integral–Derivative) merupakan kontroler yang presisi untuk menghasilkan kecepatan yang sesuai dengan referensinya. Dalam implementasi ini system PID sebagai kontrol terhadap pemicuan gate thyristor dirancang secara digital dengan menggunakan Arduino UNO. Data pengujian menunjukkan bahwa besarnya sudut pemicuan berpengaruh secara signifikan terhadap kecepatan putaran motor universal.
SISTEM INFORMASI PENGELOLAAN DATA POSYANDU NUSA INDAH V PURWAKARTA
Authors: Winarni Ade , Virki Millenia Ivena
Published: November 2023

Posyandu Nusa Indah V merupakan suatu unit layanan kesehatan yang bertugas untuk melakukan pelayanan kesehatan ibu dan anak (KIA) di wilayah RW 02 Kecamatan BabakanCikao Kabupaten Purwakarta Desa Ciwareng. Saat ini, proses pencatatan data di Posyandu Nusa Indah V masih dilakukan secara manual dengan menggunakan kerta HVS. Cara tersebut dinilai tidak efisien karena menyebabkan kesulitan dalam proses pencatatan data Posyandu Nusa Indah V. Metode penelitian yang digunakan pada tugas akhir ini adalah observasi, wawancara dengan tujuan untuk memperoleh data yang dibutuhkan dalam pembuatan sebuah program aplikasi. Dan adapun penelitian ini menggunakan model pengembangan System Development Life Cycle (SDLC) model analisis dan desain yang digunakan adalah berbasis objek dengan tools UML. Model ini mempermudah dalam pengembangan dan memudahkan user untuk memahami sistem yang akan dikembangkan. Hasil dari penelitian ini adalah terbentuknya aplikasi pendataan posyandu Nusa Indah V untuk memberikan kemudahana, kecepatan, dan ketetapan dalam melakukan pencatatan dan pendataan data serta untuk membantu mempercepat. Sehingga membantu kader dalam melakukan kegiatan di Posyandu Nusa Indah V.
Transposable elements regulate thymus development and function
Authors: Larouche Jean-David , Laumont Céline M. , Trofimov Assya , Vincent Krystel , Hesnard Leslie , Brochu Sylvie , Côté Caroline , Humeau Juliette , Bonneil Éric , Lanoix Joël , Durette Chantal , Gendron Patrick , Laverdure Jean-Philippe , Richie Ellen R. , Lemieux Sébastien , Thibault Pierre , Perreault Claude
Published: November 2023

Transposable elements (TE) are repetitive sequences representing ∼45% of the human and mouse genomes and are highly expressed by medullary thymic epithelial cells (mTEC). In this study, we investigated the role of transposable elements (TE), which are highly expressed by medullary thymic epithelial cells (mTEC), on T-cell development in the thymus. We performed multi-omic analyses of TEs in human and mouse thymic cells to elucidate their role in T cell development. We report that TE expression in the human thymus is high and shows extensive age- and cell lineage-related variations. TEs interact with multiple transcription factors in all cell types of the human thymus. Two cell types express particularly broad TE repertoires: mTECs and plasmacytoid dendritic cells (pDC). In mTECs, TEs interact with transcription factors essential for mTEC development and function (e.g., PAX1 and RELB) and generate MHC-I-associated peptides implicated in thymocyte education. Notably, AIRE, FEZF2, and CHD4 regulate non-redundant sets of TEs in murine mTECs. Human thymic pDCs homogenously express large numbers of TEs that lead to the formation of dsRNA, triggering RIG-I and MDA5 signaling and explaining why thymic pDCs constitutively secrete IFN ɑ/β. This study illustrates the diversity of interactions between TEs and the adaptive immune system. TEs are genetic parasites, and the two thymic cell types most affected by TEs (mTEcs and pDCs) are essential to establishing central T-cell tolerance. Therefore, we propose that the orchestration of TE expression in thymic cells is critical to prevent autoimmunity in vertebrates.
Tuning apico-basal polarity and junctional recycling in the hemogenic endothelium orchestrates pre-hematopoietic stem cell emergence complexity
Authors: Torcq Léa , Majello Sara , Vivier Catherine , Schmidt Anne A.
Published: November 2023

Hematopoietic stem cells emerge in the embryo from an aortic-derived tissue called the hemogenic endothelium (HE). The HE appears to give birth to cells of different nature and fate but the molecular principles underlying this complexity are largely unknown. Here we show, in the zebrafish embryo, that two cell types emerge from the aortic floor with radically different morphodynamics. With the support of live imaging, we bring evidence suggesting that the mechanics underlying the two emergence types rely, or not, on apicobasal polarity establishment. While the first type is characterized by reinforcement of apicobasal polarity and maintenance of the apical/luminal membrane until release, the second type emerges via a dynamic process reminiscent of trans-endothelial migration. Interfering with Runx1 function suggests that the balance between the two emergence types depends on tuning apicobasal polarity at the level of the HE. In addition, using new transgenic fish lines that express Junctional Adhesion Molecules and functional interference, we bring evidence for the essential role of ArhGEF11/PDZ-RhoGEF in controlling the HE-endothelial cell dynamic interface, including cell-cell intercalation, which is ultimately required for emergence completion. Overall, we highlight critical cellular and dynamic events of the endothelial-to-hematopoietic transition that support emergence complexity, with a potential impact on cell fate.Developmental Biology and Stem cells, Cell Biology.
Illuminating T cell-dendritic cell interactions in vivo by FlAsHing antigens
Authors: Akkaya Munir , Al Souz Jafar , Williams Daniel , Kamdar Rahul , Kamenyeva Olena , Kabat Juraj , Shevach Ethan M. , Akkaya Billur
Published: November 2023

Delineating the complex network of interactions between antigen-specific T cells and antigen presenting cells (APCs) is crucial for effective precision therapies against cancer, chronic infections, and autoimmunity. However, the existing arsenal for examining antigen-specific T cell interactions is restricted to a select few antigen-T cell receptor pairs, with limited in situ utility. This lack of versatility is largely due to the disruptive effects of reagents on the immune synapse, which hinder real-time monitoring of antigen-specific interactions. To address this limitation, we have developed a novel and versatile immune monitoring strategy by adding a short cysteine-rich tag to antigenic peptides that emits fluorescence upon binding to thiol-reactive biarsenical hairpin compounds. Our findings demonstrate the specificity and durability of the novel antigen-targeting probes during dynamic immune monitoring in vitro and in vivo. This strategy opens new avenues for biological validation of T-cell receptors with newly identified epitopes by revealing the behavior of previously unrecognized antigen-receptor pairs, expanding our understanding of T cell responses.
Large-scale analysis of the integration of enhancer-enhancer signals by promoters
Authors: Martinez-Ara Miguel , Comoglio Federico , van Steensel Bas
Published: November 2023

Genes are often regulated by multiple enhancers. It is poorly understood how the individual enhancer activities are combined to control promoter activity. Anecdotal evidence has shown that enhancers can combine sub-additively, additively, synergistically, or redundantly. However, it is not clear which of these modes are more frequent in mammalian genomes. Here, we systematically tested how pairs of enhancers activate promoters using a three-way combinatorial reporter assay in mouse cells. By assaying about 69,000 enhancer-enhancer-promoter combinations we found that enhancer pairs generally combine near-additively. This behaviour was conserved across seven developmental promoters tested. Surprisingly, these promoters scale the enhancer signals approximately following a power-law, but the exponent of this response varies between promoters. A housekeeping promoter showed an overall different response to enhancer pairs, and a smaller dynamic range. Thus, our data indicate that enhancers mostly act additively, but promoters transform their collective effect non-linearly.
Oh Susannah
Authors: Jacobi Maura Clement
Published: November 2023

In this narrative medicine essay, a family medicine physician whose daughter was born with a 50% chance of having the Huntingtin gene embraces the joy of the present over the fear of the future.
Initial Findings From an Acute Hospital Care at Home Waiver Initiative
Authors: Adams Danielle , Wolfe Ashby J. , Warren Jessica , Laberge Alexandre , Richards Adam C. , Herzer Kurt , Fleisher Lee A.
Published: November 2023

This cohort study assesses outcomes of patients treated during the initial 16 months of the Centers for Medicare & Medicaid Services Acute Hospital Care at Home initiative.
Use of Dexmedetomidine and Opioids in Hospitalized Preterm Infants
Authors: Curtis Samantha , Kilpatrick Ryan , Billimoria Zeenia C. , Zimmerman Kanecia , Tolia Veeral , Clark Reese , Greenberg Rachel G. , Puia-Dumitrescu Mihai
Published: November 2023

Importance

Dexmedetomidine, an α2-adrenergic agonist, is not approved by the Food and Drug Administration for use in premature infants. However, the off-label use of dexmedetomidine in premature infants has increased 50-fold in the past decade. Currently, there are no large studies characterizing dexmedetomidine use in US neonatal intensive care units (NICUs) or comparing the use of dexmedetomidine vs opioids in infants.

Objectives

To describe dexmedetomidine use patterns in the NICU and examine the association between dexmedetomidine and opioid use in premature infants.

Design, Setting, and Participants

A multicenter, observational cohort study was conducted from November 11, 2022, to April 4, 2023. Participants were inborn infants born between 22 weeks, 0 days, and 36 weeks, 6 days, of gestation at 1 of 383 Pediatrix Medical Group NICUs across the US between calendar years 2010 and 2020.

Main Outcome and Measure

Exposure to medications of interest defined as total days of exposure, timing of use, and changes over time.

Results

A total of 395 122 infants were included in the analysis. Median gestational age was 34 (IQR, 32-35) weeks, and median birth weight was 2040 (IQR, 1606-2440) g. There were 384 infants (0.1% of total; 58.9% male) who received dexmedetomidine. Infants who received dexmedetomidine were born more immature, had lower birth weight, longer length of hospitalization, more opioid exposure, and more days of mechanical ventilation. Dexmedetomidine use increased from 0.003% in 2010 to 0.185% in 2020 (P < .001 for trend), while overall opioid exposure decreased from 8.5% in 2010 to 7.2% in 2020 (P < .001 for trend). The median postmenstrual age at first dexmedetomidine exposure was 31 (IQR, 27-36) weeks, and the median postnatal age at first dexmedetomidine exposure was 3 (IQR, 1-35) days. The median duration of dexmedetomidine receipt was 6 (IQR, 2-14) days.

Conclusion and Relevance

The findings of this multicenter cohort study of premature infants suggest that dexmedetomidine use increased significantly between 2010 and 2020, while overall opioid exposure decreased. Future studies are required to further examine the short- and long-term effects of dexmedetomidine in premature and critically ill infants.
Impact of attenuation correction of radiotherapy hardware for positron emission tomography‐magnetic resonance in ano‐rectal radiotherapy patients
Authors: Wyatt Jonathan J. , Petrides George , Pearson Rachel A. , McCallum Hazel M. , Maxwell Ross J.
Published: November 2023

Background

Positron Emission Tomography‐Magnetic Resonance (PET‐MR) scanners could improve ano‐rectal radiotherapy planning through improved Gross Tumour Volume (GTV) delineation and enabling dose painting strategies using metabolic measurements. This requires accurate quantitative PET images acquired in the radiotherapy treatment position.

Purpose

This study aimed to evaluate the impact on GTV delineation and metabolic parameter measurement of using novel Attenuation Correction (AC) maps that included the radiotherapy flat couch, coil bridge and anterior coil to see if they were necessary.

Methods

Seventeen ano‐rectal radiotherapy patients received a ‐FluoroDeoxyGlucose PET‐MR scan in the radiotherapy position. PET images were reconstructed without () and with () the radiotherapy hardware included. Both AC maps used the same Computed Tomography image for patient AC. Semi‐manual and threshold GTVs were delineated on both PET images, the volumes compared and the Dice coefficient calculated. Metabolic parameters: Standardized Uptake Values , and Total Lesion Glycolysis (TLG) were compared using paired t‐tests with a Bonferroni corrected significance level of .

Results

Differences in semi‐manual GTV volumes between and were approaching statistical significance (difference , ), with larger differences in low FDG‐avid tumours (). The and GTVs were concordant with Dice coefficients (manual) and (threshold). Metabolic parameters were significantly different, with , and TLG differences of (), () and () respectively. The TLG difference resulted in 1/8 rectal cancer patients changing prognosis group, based on literature TLG cut‐offs, when using rather than .

Conclusions

This study suggests that using AC maps with the radiotherapy hardware included is feasible for patient imaging. The impact on tumour delineation was mixed and needs to be evaluated in larger cohorts. However using AC of the radiotherapy hardware is important for situations where accurate metabolic measurements are required, such as dose painting and treatment prognostication.
Impedance Measurement and Demonstrator Tests of Developed Thermally Sprayed and Sealed Coating Systems for Bearing Applications
Authors: Bobzin Kirsten , Heinemann Hendrik , Olesch Elisa , Hosenfeldt Tim , Bagcivan Nazlim , Öte Mehmet , Müller Björn , Kunde Carsten
Published: November 2023

To avoid early stage failure of rolling bearings as components of train drives of electrical engines, the bearings must be protected. As state of the art, alumina coatings are applied by atmospheric plasma spraying and subsequently an organic sealer is used to enhance the service life of these bearings under harsh conditions. This includes high applied frequencies and high ambient air humidity. For further improvement, mullite coatings and a newly developed an epoxy‐ and silicon‐based sealer applied by different sealing techniques are investigated to further enhance the electrical insulation of the bearing coatings. Impedance measurements under dry and wet conditions are conducted to analyze the capacitive behavior of the coatings. X‐ray diffraction measurements are used to correlate the influence of the present crystallographic phases of the ceramic coating on the capacitive behavior. In a following step, selected coating systems are applied onto bearings and tested in demonstrator tests. For higher currents of roughlyIBearing ≥ 0.6 A, all newly developed coatings exhibit a decreased number of electrical breakdowns compared to the reference. Consequently, choosing this coating system as electrical protection coating for bearings in electrical engines can reduce the damage occurring from electrical current breakdowns.
Development of a Novel Hierarchically Biofabricated Blood Vessel Mimic Decorated with Three Vascular Cell Populations for the Reconstruction of Small‐Diameter Arteries
Authors: Carrabba Michele , Fagnano Marco , Ghorbel Mohamed T , Rapetto Filippo , Su Bo , De Maria Carmelo , Vozzi Giovanni , Biglino Giovanni , Perriman Adam W. , Caputo Massimo , Madeddu Paolo
Published: November 2023

The availability of grafts to replace small‐diameter arteries remains an unmet clinical need. Here, the validated methodology is reported for a novel hybrid tissue‐engineered vascular graft that aims to match the natural structure of small‐size arteries. The blood vessel mimic (BVM) comprises an internal conduit of co‐electrospun gelatin and polycaprolactone (PCL) nanofibers (corresponding to the tunica intima of an artery), reinforced by an additional layer of PCL aligned fibers (the internal elastic membrane). Endothelial cells are deposited onto the luminal surface using a rotative bioreactor. A bioprinting system extrudes two concentric cell‐laden hydrogel layers containing respectively vascular smooth muscle cells and pericytes to create the tunica media and adventitia. The semi‐automated cellularization process reduces the production and maturation time to 6 days. After the evaluation of mechanical properties, cellular viability, hemocompatibility, and suturability, the BVM is successfully implanted in the left pulmonary artery of swine. Here, the BVM showed good hemostatic properties, capability to withstand blood pressure, and patency at 5 weeks post‐implantation. These promising data open a new avenue to developing an artery‐like product for reconstructing small‐diameter blood vessels.
Engineered Microcystis aerugiosa Hydrogel as an Anti‐Tumor Therapeutic by Augmenting Tumor Immunogenicity and Immune Responses
Authors: Meng Xiaoyan , Liu Zhonglong , Yang Yangzi , Li Jiaxin , Ran Zhaoyang , Zhu Yingchun , Fu Jingke , He Yue , Hao Yongqiang
Published: November 2023

Cancer immunotherapy holds great promise but is generally limited by insufficient induction of anticancer immune responses. In this work, Microcystis aerugiosa (MA) is observed to act as an immune stimulator, which activates the cGAS‐STING and IRF‐signaling pathways of dendritic cells (DCs), induces immunostimulatory factors production, eventually resulting in the death of tumor cells by promoting cytotoxic CD8+ T cells infiltration. To further enhance the tumor immunogenicity, MA is engineered with polydopamine (PDA) and assembled in injectable Pluronic F127 hydrogels (denoted as MA@PDA‐F127). The introduction of PDA endows the MA@PDA‐F127 with photothermal therapy capability, which enhances the immunogenicity by in situ exposing damage‐associated molecular patterns and tumor antigens from dying tumor cells. Importantly, the surface‐rich reaction sites in thermosensitive MA@PDA‐F127 hydrogels capture antigens, facilitating long retention of antigens exposure and enhancing DCs maturation for advanced anti‐tumor immune response. Accordingly, in situ administration of MA@PDA‐F127 hydrogels in a single dose achieves efficient eradication of both primary and distant tumors in an orthotopic tumor metastasis model. Furthermore, this hydrogel can sensitize immune checkpoint inhibitor therapy and enhance T cell infiltration in a murine tumor model. Collectively, MA@PDA‐F127 hydrogel offers an effective anti‐tumor therapeutic by augmenting tumor immunogenicity and activating a systematic immune response.
Simultaneously Enhanced Energy Harvesting and Storage Performance Achieved by 3D Mix‐Phase Mose2‐Nise/NF
Authors: He Xinrui , Sun Hailong , Li Zhipeng , Song Jie , Li Hezhang , Wang Chao , Niu Yi , Jiang Jing
Published: November 2023

As an energy harvesting device, the newly emerged thermocell has attracted great interest. However, it requires an additional energy storage device to complete a functional recycling system. As the core part of energy harvesting and storage devices, the design of electrodes with both high thermoelectric and electrochemical conversion efficiencies is particularly important. Herein, a high‐performance thermocell and supercapacitor device are constructed by employing the same mix‐phase MoSe2‐NiSe/NF electrode for both energy harvesting and storage. The thermocell exhibits an enhanced Seebeck coefficient of −1.69 mV K−1 and a superior output of 0.58 mW m−2 K−2 for low‐grade heat harvesting. Meanwhile, the supercapacitor device shows a great energy density of 54 Wh kg−1 at a power density of 806 W kg−1 and excellent cycling stability with a 92.8% retention rate after 50 000 cycles. This work offers a universal approach for the rational design of synergistic electrode materials to simultaneously achieve high‐energy harvesting and storage devices and lays a foundation for the recycling system of thermoelectric harvesting, conversion, and storage.
Improved Performance of High‐Temperature Proton Exchange Membrane Fuel Cells by Purified CNT Nanoporous Sheets
Authors: Kwon Obeen , Yang Seonghyeon , Kang Byung‐Ho , Park Junghyun , Park Gyutae , Baek Jiwon , Oh Hyoun‐Myoung , So Yoonho , Park Sung‐Hoon , Jeong Youngjin , Park Taehyun
Published: November 2023

Given environmental friendliness, high‐temperature proton exchange membrane fuel cells (HT‐PEMFCs) can be the alternative power source for clean power generation. However, neither developments nor strategies of diffusion media are actively conducted yet to achieve high‐performance HT‐PEMFCs. Herein, a nanoporous carbon nanotube (CNT) sheet, a new anode diffusion layer between the gas diffusion layer|bipolar plate interface that improves fuel cell performance and durability by CNT purification for facilitating fuel accessibility with uniform interfacial contact for reducing acid loss, is reported. Configuration of the optimal CNT‐inserted fuel cell is examined and proposed under the operating temperature of 140–200 °C. The underlying mechanism of this new diffusion layer with multiphysics simulation elucidates that the nanoporous nature induces gas‐wall collision, enabling uniform dispersion within adjacent diffusion media. These fuel cells outperform performance over two times higher and have a voltage decay rate nearly two times lower than conventional HT‐PEMFC.
Controlling the Glassy State toward Structural and Mechanical Enhancement: Additive Manufacturing of Bulk Metallic Glass Using Advanced Laser Beam Shaping Technology
Authors: Hadibeik Sepide , Ghasemi‐Tabasi Hossein , Burn Andreas , Lani Sébastien , Spieckermann Florian , Eckert Jürgen
Published: November 2023

Bulk metallic glasses (BMGs) offer exceptional physical/mechanical properties enabling them to be highly desirable for a variety of applications. Laser powder bed fusion (LPBF) has great promise for producing large and intricate BMG structures. However, using non‐optimal energy distribution in current additive manufacturing machines leads to extensive reheating of previously solidified layers. As a result, the mechanical characteristics can be significantly impacted by structural relaxation and partial crystallization. Here, a tunable advanced laser beam shaping technology is employed to overcome the difficulties originating from non‐optimal energy distribution in current additive manufacturing machines. This study fabricates fully amorphous/dense BMG samples using the shaped laser beam and established optimized atomic‐scale short‐and medium‐range ordering along with improved yield/fracture compressive strength. Formation of a shallow and wide melting pool geometry using the beam shaping allows to increase hatching distances to better control the thermal history introducing improved amorphicity and rejuvenation. This higher rejuvenation and disordering allow for increased atomic mobility, which facilitates the creation and spread of shear bands, thus enhancing the mechanical strength and ductility of the material. The current work demonstrates that BMG parts can be fabricated using flexible beam‐shaping technology allowing to go beyond the capabilities of state‐of‐the‐art additive manufacturing techniques.
A Self‐Adaptive Biomimetic Periosteum Employing Nitric Oxide Release for Augmenting Angiogenesis in Bone Defect Regeneration
Authors: Zhou Zhangzhe , Liu Yang , Li Wenjing , Zhao Zhijian , Xia Xiaowei , Liu Junlin , Deng Yaoge , Wu Yubin , Pan Xiangqiang , He Fan , Yang Huilin , Lu Weihong , Xu Yong , Zhu Xuesong
Published: November 2023

The periosteum plays a vital role in the regeneration of critical‐size bone defects and highly comminuted fractures, promoting the differentiation of osteoblasts, accelerating the reconstruction of the vascular network, and guiding bone tissue regeneration. However, the materials loaded with exogenous growth factors are limited by the release and activity of the elements. Therefore, the material structure must be carefully designed for the periosteal function. Here, a self‐adaptive biomimetic periosteum strategy is proposed, which is a novel interpenetrating double network hydrogel consisting of diselenide‐containing gelatin and calcium alginate (modified natural collagen and polysaccharide) to enhance the stability, anti‐swelling, and delayed degradation of the hydrogel. The diselenide bond continuously releases nitric oxide (NO) by metabolizing endogenous nitrosated thiols (RSNO), activates the nitric oxide‐cycle guanosine monophosphate (NO‐cGMP) signal pathway, coordinates the coupling effect of angiogenesis and osteogenesis, and accelerates the repair of bone defects. This self‐adaptive biomimetic periosteum with the interpenetrating double network structure formed by the diselenide‐containing gelatin and calcium alginate has been proven to be safe and effective in repairing critical‐size bone defects and is expected to provide a promising strategy for solving clinical problems.