bluuebunny/crossref_metadata_2025 huggingface dataset:
Special Issue of New Trends in Neuromechanics and Motor Rehabilitation
Authors: Kang Nyeonju
Published: August 2022

Neuromechanics has been focused on to investigate both behavioral characteristics and underlying neurophysiological mechanisms for various population, including healthy adults, elderly people, and patients with musculoskeletal and neurological diseases [...]
Evaluation of Land Subsidence during Groundwater Recovery
Authors: Intui Sutasinee , Inazumi Shinya , Soralump Suttisak
Published: August 2022

The Chao Phraya River basin is located in the central area of Thailand, which experiences many land subsidence issues due to groundwater pumping. The Department of Groundwater Resources (DGR) has been recording data on the changes in the groundwater level due to water pumping since 1960 until the present time. In 1997, the DGR issued a law regulating the use of groundwater due to its effect on the changes in the groundwater level. The changing of the groundwater level was separated into two periods. The first period is the high groundwater pumping ratio that led to a rapid decrease in the groundwater level of about 27 m from the ground surface. After the DGR issued the new law in the second period, the pumping ratio decreased and the groundwater level increased. The groundwater level tends to reach the ground surface. In the past, the groundwater level decrease was affected by the land subsidence. Therefore, this study focused on calculating and learning the behavior of the soil surface displacement during groundwater level recovery to the ground surface in Bangkok, Thailand. We obtained the 3D soil profiles adopted from eight boreholes from the Department of Public Works and Town & Country Planning. The soil profile data were verified by monitoring the data from the Department of Groundwater Resources (DGR) in the same area. The soil layers of the 3D soil profile were analyzed to calculate the soil surface displacement based on the consolidation theory of Terzaghi. We also examined the displacement behavior of the clay layers during the groundwater level recovery to the ground surface by assuming that the soil layers below the groundwater level do not settle or rebound. The surface displacement results showed that the surface ends to move upward or rebound, which is a similar trend to that reported in previous research. All the considered locations showed similar soil surface displacement trends. The soil displacement ratio is 0.21 to 0.53 cm/year during the groundwater recovery.
Analysis of Dynamic Behavior of ParReEx Robot Used in Upper Limb Rehabilitation
Authors: Tarnita Daniela , Geonea Ionut Daniel , Pisla Doina , Carbone Giuseppe , Gherman Bogdan , Tohanean Nicoleta , Tucan Paul , Abrudan Cristian , Tarnita Danut Nicolae
Published: August 2022

This paper presents a dynamic analysis of the ParReEx multibody mechanism, which has been designed for human wrist joint rehabilitation. The starting point of the research is a virtual prototype of the ParReEx multibody mechanism. This model is used to simulate the dynamics of the multibody mechanism using ADAMS in three simulation scenarios: (a) rigid kinematic elements without friction in joints, (b) rigid kinematic elements with friction in joints, and (c) kinematic elements as deformable solids with friction in joints. In all three cases, the robot is used by a virtual patient in the form of a mannequin. Results such as the connecting forces in the kinematic joints and the torques necessary to operate the ParReEx robot modules are obtained by dynamic simulation in MSC.ADAMS. The torques obtained by numerical simulation are compared with those obtained experimentally. Finite element structural optimization (FEA) of the flexion/extension multibody mechanism module is performed. The results demonstrate the operational safety of the ParReEx multibody mechanism, which is structurally capable of supporting the external loads to which it is subjected.
Coupled Dynamic Analysis of a Bottom-Fixed Elastic Platform with Wave Energy Converters in Random Waves
Authors: Heo Sanghwan , Koo Weoncheol , Kim Moo-Hyun
Published: August 2022

A Wavestar-type Wave Energy Converter (WEC) on an elastic foundation structure was investigated using an author-developed coupled dynamic analysis computer program. The program included an elastic foundation structure composed of beam elements, a multi-body dynamics model of the entire system, a hydrodynamics model of the dual-buoy, and fully coupled dynamics considering the interaction between the structure and WECs. The selected WEC models a heaving-point-absorber (HPA), one of the oscillating body systems which causes rotational motions of a connecting rod attached to the foundation structure. A rotational-damper-type hydraulic power take-off (PTO) system on the foundation structure produced electricity. The bottom-fixed foundation structure was modeled by three-dimensional beam elements, and the entire system, including HPA, was analyzed by multi-body dynamics. Random wave data at Buan, a nearshore region of Korea, collected by the Korea Meteorological Administration (KMA), was used as a demonstration study using the developed computer programs. Through the case study, the displacement and stress of the foundation structure were increased significantly by the dynamic coupling effects with the WECs, which underscores that the coupled dynamic analysis is essential for a reliable performance evaluation and the design of such a system.
Effects of Cardiac Contractility Modulation Therapy on Right Ventricular Function: An Echocardiographic Study
Authors: Contaldi Carla , De Vivo Stefano , Martucci Maria L. , D’Onofrio Antonio , Ammendola Ernesto , Nigro Gerardo , Errigo Vittoria , Pacileo Giuseppe , Masarone Daniele
Published: August 2022

Background. Cardiac contractility modulation (CCM) is a novel device-based therapy for patients with heart failure with reduced and mild reduced ejection fraction (HFrEF/HFmrEF). CCM increases cardiac performance and produces reverse left ventricular remodeling, with improved symptoms, functional capacity, quality of life, and reduced HF-related hospitalizations. However, to date, little evidence is available on the effects of CCM on right ventricle (RV) function. Therefore, we analyzed the effects of CCM on RV systolic function and RV–pulmonary artery (PA) coupling. Methods. Twenty-one (65 ± 12.5 years) patients with NYHA class III, ejection fraction < 40% and QRS < 120 ms were assessed at baseline. During follow up, two patients had died, and so nineteen patients were evaluated six months after CCM therapy. Using echocardiography, tricuspid annular systolic excursion (TAPSE), myocardial systolic excursion velocity (RVs), and RV free-wall strain was measured. PA systolic pressure (PASP) was estimated from tricuspid regurgitation, adding the right atrial pressure estimation. The RV-PA coupling was calculated as TAPSE/PASP ratio. Results. After six months, patients who underwent CCM therapy showed a reduction in RV diameters and improved RV systolic function, as evidenced by the increase in both TAPSE (16.6 ± 4.2 mm vs. 18.5 ± 3.6 mm; p < 0.05), RVs (10.1 ± 1.8 cm/s vs. 11.3 ± 11.4 cm/s; p < 0.05), and RV strain (−13.7 ± 1.8% vs. −15.6 ± 2.3%; p < 0.05). CCM also determined a reduction in PASP (34.2 ± 9.8 mmHg vs. 28 ± 6.2 mmHg; p < 0.05) and an increase in the TAPSE/PASP ratio (0.52 ± 0.14 mm/mmHg vs. 0.66 ± 0.23 mm/mmHg; p < 0.05). Conclusions. At six months, CCM increases RV reverse remodeling and performance, reducing RV size and improving RV systolic function, PASP, and RV-PA coupling.
Extension Design Model of Rapid Configuration Design for Complex Mechanical Products Scheme Design
Authors: Wang Tichun , Li Hao , Wang Xianwei
Published: August 2022

This study explores the extension configuration methods of complex product conceptual design, seeking to improve the product design efficiency and design quality. The paper firstly reviews the literature on element representation models of multi-type design knowledge, followed by a review on extension design models for the rapid configuration of complex product conceptual design. The extension transformation method for the rapid configuration design of complex product conceptual design is also reviewed. With the analysis of the extension reasoning model for the rapid configuration design of complex product conceptual design, the research proposes a new model of extension reasoning for the rapid configuration design of complex product conceptual design. This model of extension design would enhance the rapid configuration design and conceptual design of large and complex products. Detailed steps of the algorithm implementation are also presented. This study also tests the validity and operability of the model and the algorithm with the design case of a large hydro-turbine product design.
Soft Computing Techniques Aware Clustering-Based Routing Protocols in Vehicular Ad Hoc Networks: A Review
Authors: Sindhwani Manoj , Sachdeva Shippu , Arora Krishan , Yoon Taehyun , Yoo Daeseung , Joshi Gyanendra Prasad , Cho Woong
Published: August 2022

The vehicular ad hoc network is an emerging area of technology that provides intelligent transportation systems with vast advantages and applications. Frequent disconnections between the vehicular nodes due to high-velocity vehicles impact network performance. This can be addressed by efficient clustering techniques. Several recent studies have attempted to develop optimal clustering algorithms to improve network performance metrics using soft computing techniques. Although sufficient work on soft computing techniques has been carried out, it seems less commonplace to find an analysis of various algorithms’ network parameters together. This paper provides a systematic analysis of the clustering-based routing protocols used in vehicular networks that are aware of soft computing techniques. The categorization is performed according to various soft computing techniques: particle swarm optimization, k-means, neural networks, artificial bee colony, genetic algorithm, firefly algorithm, and fuzzy logic. A comparative study of soft computing strategies is also provided in the survey with a focus on their objectives, along with their strengths and limitations. This survey makes it easier for researchers to pick the required soft computing technique used in vehicular networks in order to improve metrics such as packet delivery ratio, end-to-end delay, throughput, cluster lifetime, and message overhead.
A Parameterized Design Method for Building a Shading System Based on Climate Adaptability
Authors: Wang Shiliang , Zhang Qun , Liu Peng , Liang Rui , Fu Zitian
Published: August 2022

The relationship between environmental factors and the indoor physical environment is very close, and external shading is considered an effective way to adjust the interaction between the indoor and outdoor environment. However, determining how to set up an external shading system remains a notable issue. In the early design stage, architects have adopted the process of designing the form and function first and then checking whether those characteristics meet the energy-saving specifications. However, this process involves a great deal of repetitive and inefficient work and cannot meet the requirements of energy savings and emission reductions in a global context. Therefore, it is particularly important to seek a design method that combines energy-saving design with form-based design. This paper takes a construction project in Northwest China as its research object. In this study, typical parametric models for external shading are designed. Furthermore, indoor performance objectives based on light environment analysis are proposed, and Ladybug Tools and the genetic algorithm (GA) are used for optimization and verification. The optimization results show that the adaptive shading system can significantly reduce the total cooling energy consumption per unit area in summer by 20% and 15%, respectively. The comfort level throughout the year improved by 14.8% (air conditioning on) and 4.7% (air conditioning off). This study proposes a fast and effective shading parametric design method for architects in the early stage, improving the efficiency and accuracy of performance-based design.
Investigation of the Gas-Phase Reaction of Nopinone with OH Radicals: Experimental and Theoretical Study
Authors: El Dib Gisèle , Mano Priya Angappan , Lakshmipathi Senthilkumar
Published: August 2022

Monoterpenes are the most essential reactive biogenic volatile organic compounds. Their removal from the atmosphere leads to the formation of oxygenated compounds, such as nopinone (C9H14O), one of the most important first-generation β-pinene oxidation products that play a pivotal role in environmental and biological applications. In this study, experimental and theoretical rate coefficients were determined for the gas-phase reaction of nopinone with hydroxyl radicals (OH). The absolute rate coefficient was measured for the first time using a cryogenically cooled cell along with the pulsed laser photolysis–laser-induced fluorescence technique at 298 K and 7 Torr. The hydrogen abstraction pathways were found by using electronic structure calculations to determine the most favourable H-abstraction position. Pathway 5 (bridgehead position) was more favourable, with a small barrier height of −1.23 kcal/mol. The rate coefficients were calculated based on the canonical variational transition state theory with the small-curvature tunnelling method (CVT/SCT) as a function of temperature. The average experimental rate coefficient (1.74 × 10−11 cm3 molecule−1 s−1) was in good agreement with the theoretical value (2.2 × 10−11 cm3 molecule−1 s−1). Conclusively, the results of this study pave the way to understand the atmospheric chemistry of nopinone with OH radicals.
Development of an Innovative Procedure for Lithium Plating Limitation and Characterization of 18650 Cycle Aged Cells for DCFC Automotive Applications
Authors: Dotoli Matteo , Milo Emanuele , Giuliano Mattia , Tiozzo Arianna , Baricco Marcello , Nervi Carlo , Ercole Massimiliano , Sgroi Mauro Francesco
Published: August 2022

Since lithium-ion batteries seem to be the most eligible technology to store energy for e-mobility applications, it is fundamental to focus attention on kilometric ranges and charging times. The optimization of the charging step can provide the appropriate tradeoff between time saving and preserving cell performance over the life cycle. The implementation of new multistage constant current profiles and related performances after 1000 cycles are presented and compared with respect to a reference profile. A physicochemical (SEM, XRD, particle size analysis, etc.) and electrochemical (incremental capacity analysis, internal resistance measurements) characterization of the aged cells is shown and their possible implementation on board is discussed.
Cultured Autologous Corneal Epithelia for the Treatment of Unilateral Limbal Stem Cell Deficiency: A Case Series of 15 Patients
Authors: Guérin Louis-Philippe , Larouche Danielle , Morcos Mohib W. , Faucher Anne , Auger François A. , Knoppers Bartha M. , Kyrillos Ralph , Bazin Richard , Germain Lucie
Published: August 2022

Damage to limbal epithelial stem cells can lead to limbal stem cell deficiency (LSCD). Current autologous treatment procedures for unilateral LSCD bear a significant risk of inducing LSCD in the donor eye. This complication can be avoided by grafting a stem cell containing cultured autologous corneal epithelium (CACE). The primary objective of this study was to demonstrate the safety of CACE grafted on eyes with LSCD. The secondary objective was to assess the efficacy of a CACE graft in restoring a self-renewing corneal surface with adequate anatomic structures, as well as improving the best corrected visual acuity (BCVA). Fifteen patients were grafted with a CACE on a fibrin gel produced from a 3 mm2 limbal biopsy harvested from the donor eye. Data were collected at baseline and after grafting. Follow-ups from 1 to 5 years were conducted. No major adverse events related to the CACE graft were observed. For every visit, an anatomic score based on corneal opacity as well as central vascularization and a functional score based on BCVA were determined. Safety was demonstrated by the low occurrence of complications. Anatomical (93%) and functional (47%) results are promising for improving vision in LSCD patients. Combined functional success and partial success rates with inclusion of BCVA were 53% [CI95: 27–79%] one year after CACE grafting. At the last follow-up, 87% [CI95: 60–98%] of the patients had attained corneal clarity. The outcomes demonstrate the safety of our technique and are promising regarding the efficacy of CACE in these patients.
Plasma Levels of Proprotein Convertase Subtilisin/Kexin Type 9 Are Inversely Associated with N-Terminal Pro B-Type Natriuretic Peptide in Older Men and Women
Authors: Spannella Francesco , Giulietti Federico , Galeazzi Roberta , Passarelli Anna , Re Serena , Di Pentima Chiara , Allevi Massimiliano , Magni Paolo , Sarzani Riccardo
Published: August 2022

Background and Aims: Cardiac natriuretic peptides (NPs) exert several metabolic effects, including some on lipid metabolism. Higher NPs levels are likely to be associated with a favorable lipid profile. In in vitro studies, NPs have been found to modulate low-density lipoprotein receptor (LDLR) trafficking by preventing proprotein convertase subtilisin/kexin type 9 (PCSK9) overexpression. The aim of our study is to investigate a possible association between plasma levels of PCSK9 and N-terminal pro B-type natriuretic peptide (NT-proBNP) in vivo. Methods: We performed a cross-sectional study on 160 consecutive older male and female patients hospitalized for medical conditions. Patients taking lipid-lowering drugs and patients with an admission diagnosis of acute heart failure were excluded. Fasting blood samples were collected after clinical stabilization of the acute illness, the day before discharge. Results: The mean age was 87.8 ± 6.4 years with a female prevalence (62.5%). The median NT-proBNP was 2340 (814–5397) pg/mL. The mean plasma PCSK9 was 275.2 ± 113.2 ng/mL. We found an inverse correlation between plasma PCSK9 and NT-proBNP (r = −0.280; p = 0.001). This association was confirmed after taking into account NT-proBNP tertiles (plasma PCSK9 levels: 317.4 ± 123.6 ng/mL in the first tertile, 283.3 ± 101.8 ng/mL in the second tertile, 231.3 ± 99.0 ng/mL in the third tertile, p = 0.001) and even after an adjustment for confounding factors (beta = −0.361, p = 0.001 for ln(NT-proBNP); beta = −0.330, p = 0.001 for NT-proBNP tertiles). The strength of the correlation between plasma PCSK9 and NT-proBNP was likely greater in patients affected by type 2 diabetes mellitus (r = −0.483; p = 0.006) and in male patients (r = −0.431, p = 0.001). Conclusion: The inverse association found between PCSK9 and NT-proBNP plasma levels in our real-life clinical study supports the hypothesis that NPs may play a role in cholesterol metabolism, possibly through an inhibitory action on circulating PCSK9 concentrations, thus increasing the availability of LDLR.
Fluorescent In Situ Staining and Flow Cytometric Procedures as New Pre-Diagnostic Tests for Sialidosis, GM1 Gangliosidosis and Niemann–Pick Type C
Authors: Capitini Claudia , Feo Federica , Caciotti Anna , Tonin Rodolfo , Lulli Matteo , Coviello Domenico , Guerrini Renzo , Calamai Martino , Morrone Amelia
Published: August 2022

Background: Early diagnosis is essential in the field of lysosomal storage disorders for the proper management of patients and for starting therapies before irreversible damage occurs, particularly in neurodegenerative conditions. Currently, specific biomarkers for the diagnosis of lysosomal storage disorders are lacking in routine laboratory practice, except for enzymatic tests, which are available only in specialized metabolic centers. Recently, we established a method for measuring and verifying changes in GM1 ganglioside levels in peripheral blood lymphocytes in patients with GM1 gangliosidosis. However, fresh blood is not always available, and using frozen/thawed lymphocytes can lead to inaccurate results. Methods: We used frozen/thawed fibroblasts obtained from stored biopsies to explore the feasibility of fluorescent imaging and flow-cytometric methods to track changes in storage materials in fibroblasts from patients with three lysosomal neurodegenerative conditions: GM1 gangliosidosis, Sialidosis, and Niemann–Pick type C. We used specific markers for each pathology. Results and Conclusions: We demonstrated that with our methods, it is possible to clearly distinguish the levels of accumulated metabolites in fibroblasts from affected and unaffected patients for all the three pathologies considered. Our methods proved to be rapid, sensitive, unbiased, and potentially applicable to other LSDs.
CCL4 Deletion Accelerates Wound Healing by Improving Endothelial Cell Functions in Diabetes Mellitus
Authors: Chang Ting-Ting , Chen Ching , Lin Liang-Yu , Chen Jaw-Wen
Published: August 2022

Chronic inflammation in diabetes mellitus (DM) is the leading cause of non-healing wounds. Chemokine CC motif ligand 4 (CCL4) is enhanced in the circulation and in the wounds of DM patients. This study aimed to investigate the effect of endogenous CCL4 inhibition on diabetic wound healing. Endothelial progenitor cells (EPCs) and human dermal microvascular endothelial cells (HDMECs) were used. Mice were injected with streptozotocin to generate hyperglycemia. An enhanced CCL4 level as well as decreased tube formation and migration abilities were observed in high-glucose-treated HDMECs and in EPCs from type 2 DM patients. CCL4 inhibition by siRNA restored the damaged cell function by upregulating the Akt/endothelial nitric oxide synthase/vascular endothelial growth factor/stromal cell-derived factor-1α pathways. Wild-type diabetic mice had delayed wound repair, whereas the CCL4-knockout diabetic mice showed an accelerated rate of wound closure. In a Matrigel plug assay, CCL4-knockout diabetic mice showed higher blood vessel and hemoglobin levels. Higher CD31 and Ki67 expression in the wound area and Matrigel plugs was detected in the CCL4-knockout diabetic mice. CCL4-knockout mice had upregulated angiogenic factors and downregulated inflammatory factors. This study might provide the theoretical basis for CCL4 inhibition as a therapeutic option for clinical diabetic wound treatment.
Progressive Vascular Abnormalities in the Aging 3xTg-AD Mouse Model of Alzheimer’s Disease
Authors: Jullienne Amandine , Quan Ryan , Szu Jenny I. , Trinh Michelle V. , Behringer Erik J. , Obenaus Andre
Published: August 2022

Vascular dysfunction and structural abnormalities in Alzheimer’s disease (AD) are known to contribute to the progression of the pathology, and studies have tended to ignore the role of the vasculature in AD progression. We utilized the 3xTg-AD mouse model of AD to examine individual cerebral vessels and the cortical vascular network across the lifespan. Our vessel painting approach was used to label the entire cortical vasculature, followed by epifluorescence microscopy. The middle cerebral artery (MCA) tree was assessed with confocal microscopy, and a new method was developed to assess branching patterns as a measure of aging-related changes. We found that vascular remodeling was profoundly altered at 4–6 months of age, when the 3xTg-AD mouse is known to transition to cognitive impairment and Aβ deposition in both sexes. Analysis of vascular features (density, junctions, length) of the MCA territory highlighted sex-dependent differences across the 3xTg-AD mouse lifespan, with no alterations in branching patterns. Our current cerebrovascular angioarchitectural analyses demonstrate progressive alterations in individual cortical vessels, as well as in the vascular network of the cortex. These new findings advance our understanding of brain anatomy and physiology in the 3xTg-AD mouse, while potentially identifying unique diagnostic signatures of AD progression.
Chromosome Translocations, Gene Fusions, and Their Molecular Consequences in Pleomorphic Salivary Gland Adenomas
Authors: Stenman Göran , Fehr Andre , Skálová Alena , Vander Poorten Vincent , Hellquist Henrik , Mikkelsen Lauge Hjorth , Saba Nabil F. , Guntinas-Lichius Orlando , Chiesa-Estomba Carlos Miguel , Andersson Mattias K. , Ferlito Alfio
Published: August 2022

Salivary gland tumors are a heterogeneous group of tumors originating from the major and minor salivary glands. The pleomorphic adenoma (PA), which is the most common subtype, is a benign lesion showing a remarkable morphologic diversity and that, upon recurrence or malignant transformation, can cause significant clinical problems. Cytogenetic studies of >500 PAs have revealed a complex and recurrent pattern of chromosome rearrangements. In this review, we discuss the specificity and frequency of these rearrangements and their molecular/clinical consequences. The genomic hallmark of PA is translocations with breakpoints in 8q12 and 12q13-15 resulting in gene fusions involving the transcription factor genes PLAG1 and HMGA2. Until recently, the association between these two oncogenic drivers was obscure. Studies of the Silver–Russel syndrome, a growth retardation condition infrequently caused by mutations in IGF2/HMGA2/PLAG1, have provided new clues to the understanding of the molecular pathogenesis of PA. These studies have demonstrated that HMGA2 is an upstream regulator of PLAG1 and that HMGA2 regulates the expression of IGF2 via PLAG1. This provides a novel explanation for the 8q12/12q13-15 aberrations in PA and identifies IGF2 as a major oncogenic driver and therapeutic target in PA. These studies have important diagnostic and therapeutic implications for patients with PA.
Microneedle-Based Glucose Sensor Platform: From Vitro to Wearable Point-of-Care Testing Systems
Authors: Ju Jian , Li Lin , Regmi Sagar , Zhang Xinyu , Tang Shixing
Published: August 2022

Significant advanced have recently been made in exploiting microneedle-based (MN-based) diabetes devices for minimally invasive wearable biosensors and for continuous glucose monitoring. Within this emerging class of skin-worn MN-based sensors, the ISF can be utilized as a rich biomarker source to diagnose diabetes. While initial work of MN devices focused on ISF extraction, the recent research trend has been oriented toward developing in vivo glucose sensors coupled with optical or electrochemical (EC) instrumentation. This outlook highlights the essential characteristics of the sensing mechanisms, rational design, sensing properties, and applications. Finally, we describe the opinions about the challenge and prospects of optical and EC MN-based device platforms for the fabrication of wearable biosensors and their application potential in the future.
Current Perspectives in Graphene Oxide-Based Electrochemical Biosensors for Cancer Diagnostics
Authors: Ozkan-Ariksoysal Dilsat
Published: August 2022

Since the first commercial biosensor device for blood glucose measurement was introduced in the 1970s, many “biosensor types” have been developed, and this research area remains popular worldwide. In parallel with some global biosensor research reports published in the last decade, including a great deal of literature and industry statistics, it is predicted that biosensor design technologies, including handheld or wearable devices, will be preferred and highly valuable in many areas in the near future. Biosensors using nanoparticles still maintain their very important place in science and technology and are the subject of innovative research projects. Among the nanomaterials, carbon-based ones are considered to be one of the most valuable nanoparticles, especially in the field of electrochemical biosensors. In this context, graphene oxide, which has been used in recent years to increase the electrochemical analysis performance in biosensor designs, has been the subject of this review. In fact, graphene is already foreseen not only for biosensors but also as the nanomaterial of the future in many fields and is therefore drawing research attention. In this review, recent and prominent developments in biosensor technologies using graphene oxide (GO)-based nanomaterials in the field of cancer diagnosis are briefly summarized.
Use of Riluzole for the Treatment of Hereditary Ataxias: A Systematic Review
Authors: Ayala Iván Nicolas , Aziz Syed , Argudo Jennifer M. , Yepez Mario , Camacho Mikaela , Ojeda Diego , Aguirre Alex S. , Oña Sebastian , Andrade Andres F. , Vasudhar Ananya , Moncayo Juan A. , Hassen Gashaw , Ortiz Juan Fernando , Tambo Willian
Published: August 2022

Ataxia is a constellation of symptoms that involves a lack of coordination, imbalance, and difficulty walking. Hereditary ataxia occurs when a person is born with defective genes, and this degenerative disorder may progress for several years. There is no effective cure for ataxia, so we need to search for new treatments. Recently, interest in riluzole in the treatment of ataxia has emerged. We conducted this systematic review to analyze the safety and efficacy of riluzole for treating hereditary ataxia in recent clinical trials. We conducted a systematic review using PubMed and Google Scholar as databases in search of this relationship. We used the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) and Meta-analysis of Observational Studies in Epidemiology (MOOSE) protocols to conduct this study. For inclusion criteria, we included full-text clinical trials on humans written in English and found three clinical trials. We excluded case reports, literature reviews, systematic reviews, and meta-analyses for this analysis. We aimed to evaluate the Scale for the Assessment and Rating of Ataxia (SARA) score, the International Cooperative Ataxia Rating Scale (ICARS) score, and the safety of the medication. Two out of the three clinical trials showed statistically significant clinical improvement in the ICARS and SARA scores, while the other trial did not show improvement in the clinical or radiological outcomes. The drug was safe in all clinical trials. Overall, the results of this analysis of riluzole for the treatment of hereditary ataxia are encouraging. Further clinical trials are needed to investigate the efficacy of riluzole on hereditary ataxia.
Research on Soft Flutter of 420m-Span Pedestrian Suspension Bridge and Its Aerodynamic Measures
Authors: Guan Qinghai , Liu Lei , Gao Hui , Wang Yujing , Li Jiawu
Published: August 2022

In order to study the flutter of long-span pedestrian suspension bridge and its aerodynamic control, a 420m-span pedestrian suspension bridge is used as an engineering example, the wind-induced vibration of seven particular aerodynamic sections is studied by wind tunnel tests, and the soft flutter phenomenon of two kinds of aerodynamic sections is identified. The results show that the wind fairing and the wind-retaining plate measures are not necessarily effective measures to improve the wind-induced stability of long-span pedestrian suspension bridge, as these two measures may reduce the flutter stability: the wind fairing section in the positive angle of attack is prone to torsion-based soft flutter phenomenon, in which the vertical vibration spectrum contains multiple vibration frequencies, so the conventional formulation of the linearized self-excited forces is no longer satisfied; the wind-retaining plate section in the negative angle of attack is prone to soft flutter dominated by vertical vibration, and the beat vibration phenomenon is found in the torsional vibration time history of the wind-retaining section. Slotting in the center of the girder section can significantly change the flow state of the section, which is an effective measure to improve the flutter stability of the pedestrian suspension bridge.
Construction Tasks Electronic Process Monitoring: Laboratory Circuit-Based Simulation Deployment
Authors: Calvetti Diego , Sanhudo Luís , Mêda Pedro , Martins João Poças , Gonçalves Miguel Chichorro , Sousa Hipólito
Published: August 2022

The domain of data processing is essential to accelerate the delivery of information based on electronic performance monitoring (EPM). The classification of the activities conducted by craft workers can enhance the mechanisation and productivity of activities. However, research in this field is mainly based on simulations of binary activities (i.e., performing or not performing an action). To enhance EPM research in this field, a dynamic laboratory circuit-based simulation of ten common constructions activities was performed. A circuit feasibility case study of EPM using wearable devices was conducted, where two different data processing approaches were tested: machine learning and multivariate statistical analysis (MSA). Using the acceleration data of both wrists and the dominant leg, the machine-learning approach achieved an accuracy between 92 and 96%, while MSA achieved 47–76%. Additionally, the MSA approach achieved 32–76% accuracy by monitoring only the dominant wrist. Results highlighted that the processes conducted with manual tools (e.g., hammering and sawing) have prominent dominant-hand motion characteristics that are accurately detected with one wearable. However, free-hand performing (masonry), walking and not operating value (e.g., sitting) require more motion analysis data points, such as wrists and legs.
Excessive Oxalic Acid Secreted by Sparassis latifolia Inhibits the Growth of Mycelia during Its Saprophytic Process
Authors: Shu Lili , Wang Miaoyue , Wang Shuang , Li Yu , Xu Hui , Qiu Zhiheng , Li Tianlai
Published: August 2022

Sparassis latifolia is an edible and medicinal mushroom in Asia commercially cultivated on substrates containing pine sawdust. Its slow mycelial growth rate greatly increases the cultivation cycle. In this study, we mainly studied the role of oxalic acid (OA) secreted by S. latifolia in its saprophytic process. Our results show that crystals observed on the mycelial surface contained calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) according to X-ray diffraction (XRD). Vegetative mycelia secreted large amounts of OA during extended culture periods. However, high concentrations of OA decreased the mycelial growth rate significantly. Moreover, the degradation of lignocellulose was significantly inhibited under high concentrations of OA. These changes could be attributed to the significantly decreased activities of lignocellulose-degrading enzymes. In conclusion, by establishing a link between OA secretion by the mycelium and the slow growth rate of its saprophytic process, this work provides fundamental information for shortening the cultivation cycle of S. latifolia.
Cross Talk between Hydrogen Peroxide and Nitric Oxide in the Unicellular Green Algae Cell Cycle: How Does It Work?
Authors: Pokora Wojciech , Tułodziecki Szymon , Dettlaff-Pokora Agnieszka , Aksmann Anna
Published: August 2022

The regulatory role of some reactive oxygen species (ROS) and reactive nitrogen species (RNS), such as hydrogen peroxide or nitric oxide, has been demonstrated in some higher plants and algae. Their involvement in regulation of the organism, tissue and single cell development can also be seen in many animals. In green cells, the redox potential is an important photosynthesis regulatory factor that may lead to an increase or decrease in growth rate. ROS and RNS are important signals involved in the regulation of photoautotrophic growth that, in turn, allow the cell to attain the commitment competence. Both hydrogen peroxide and nitric oxide are directly involved in algal cell development as the signals that regulate expression of proteins required for completing the cell cycle, such as cyclins and cyclin-dependent kinases, or histone proteins and E2F complex proteins. Such regulation seems to relate to the direct interaction of these signaling molecules with the redox-sensitive transcription factors, but also with regulation of signaling pathways including MAPK, G-protein and calmodulin-dependent pathways. In this paper, we aim to elucidate the involvement of hydrogen peroxide and nitric oxide in algal cell cycle regulation, considering the role of these molecules in higher plants. We also evaluate the commercial applicability of this knowledge. The creation of a simple tool, such as a precisely established modification of hydrogen peroxide and/or nitric oxide at the cellular level, leading to changes in the ROS-RNS cross-talk network, can be used for the optimization of the efficiency of algal cell growth and may be especially important in the context of increasing the role of algal biomass in science and industry. It could be a part of an important scientific challenge that biotechnology is currently focused on.
Phenformin Down-Regulates c-Myc Expression to Suppress the Expression of Pro-Inflammatory Cytokines in Keratinocytes
Authors: Liu Guanyi , Li Dingyang , Zhang Liwei , Xu Qiuping , Zhuang Dexuan , Liu Panpan , Hu Ling , Deng Huiting , Sun Jianfeng , Wang Shuangshuang , Zheng Bin , Guo Jing , Wu Xunwei
Published: August 2022

The treatment of many skin inflammation diseases, such as psoriasis and atopic dermatitis, is still a challenge and inflammation plays important roles in multiple stages of skin tumor development, including initiation, promotion and metastasis. Phenformin, a biguanide drug, has been shown to play a more efficient anti-tumor function than another well-known biguanide drug, metformin, which has been reported to control the expression of pro-inflammatory cytokines; however, little is known about the effects of phenformin on skin inflammation. This study used a mouse acute inflammation model, ex vivo skin organ cultures and in vitro human primary keratinocyte cultures to demonstrate that phenformin can suppress acute skin inflammatory responses induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in vivo and significantly suppresses the pro-inflammatory cytokines IL-1β, IL-6 and IL-8 in human primary keratinocytes in vitro. The suppression of pro-inflammatory cytokine expression by phenformin was not directly through regulation of the MAPK or NF-κB pathways, but by controlling the expression of c-Myc in human keratinocytes. We demonstrated that the overexpression of c-Myc can induce pro-inflammatory cytokine expression and counteract the suppressive effect of phenformin on cytokine expression in keratinocytes. In contrast, the down-regulation of c-Myc produces effects similar to phenformin, both in cytokine expression by keratinocytes in vitro and in skin inflammation in vivo. Finally, we showed that phenformin, as an AMPK activator, down-regulates the expression of c-Myc through regulation of the AMPK/mTOR pathways. In summary, phenformin inhibits the expression of pro-inflammatory cytokines in keratinocytes through the down-regulation of c-Myc expression to play an anti-inflammation function in the skin.
Macrophage Phenotypes in Normal and Diabetic Wound Healing and Therapeutic Interventions
Authors: Al Sadoun Hadeel
Published: August 2022

Macrophage differentiation and polarization are essential players in the success of the wound-healing process. Acute simple wounds progress from inflammation to proliferation/regeneration and, finally, to remodeling. In injured skin, macrophages either reside in the epithelium or are recruited from monocytes. Their main role is supported by their plasticity, which allows them to adopt different phenotypic states, such as the M1-inflammatory state, in which they produce TNF and NO, and the M2-reparative state, in which they resolve inflammation and exhibit a reparative function. Reparative macrophages are an essential source of growth factors such as TGF-β and VEGF and are not found in nonhealing wounds. This review discusses the differences between macrophage phenotypes in vitro and in vivo, how macrophages originate, and how they cross-communicate with other cellular components in a wound. This review also highlights the dysregulation of macrophages that occurs in nonhealing versus overhealing wounds and fibrosis. Then, the therapeutic manipulation of macrophages is presented as an attractive strategy for promoting healing through the secretion of growth factors for angiogenesis, keratinocyte migration, and collagen production. Finally, Hoxa3 overexpression is discussed as an example of the therapeutic repolarization of macrophages to the normal maturation state and phenotype with better healing outcomes.
Add-On Cyclic Angiotensin-(1-7) with Cyclophosphamide Arrests Progressive Kidney Disease in Rats with ANCA Associated Glomerulonephritis
Authors: Cerullo Domenico , Rottoli Daniela , Corna Daniela , Abbate Mauro , Benigni Ariela , Remuzzi Giuseppe , Zoja Carlamaria
Published: August 2022

Rapidly progressive crescentic glomerulonephritis associated with anti-neutrophil cytoplasmic antibodies (ANCA-GN) is a major cause of renal failure. Current immunosuppressive therapies are associated with severe side effects, intensifying the need for new therapeutic strategies. The activation of Mas receptor/Angiotensin-(1-7) axis exerted renoprotection in chronic kidney disease. Here, we investigated the effect of adding the lanthionine-stabilized cyclic form of angiotensin-1-7 [cAng-(1-7)] to cyclophosphamide in a rat model of ANCA-GN. At the onset of proteinuria, Wistar Kyoto rats with ANCA-GN received vehicle or a single bolus of cyclophosphamide, with or without daily cAng-(1-7). Treatment with cAng-(1-7) plus cyclophosphamide reduced proteinuria by 85% vs. vehicle, and by 60% vs. cyclophosphamide, and dramatically limited glomerular crescents to less than 10%. The addition of cAng-(1-7) to cyclophosphamide protected against glomerular inflammation and endothelial rarefaction and restored the normal distribution of parietal epithelial cells. Ultrastructural analysis revealed a preserved GBM, glomerular endothelium and podocyte structure, demonstrating that combination therapy provided an additional layer of renoprotection. This study demonstrates that adding cAng-(1-7) to a partially effective dose of cyclophosphamide arrests the progression of renal disease in rats with ANCA-GN, suggesting that cAng-(1-7) could be a novel clinical approach for sparing immunosuppressants.
Role of Intestinal Dysbiosis and Nutrition in Rheumatoid Arthritis
Authors: Attur Malavikalakshmi , Scher Jose U , Abramson Steven B. , Attur Mukundan
Published: August 2022

Rheumatoid arthritis is a chronic systemic immune-mediated disease caused by genetic and environmental factors. It is often characterized by the generation of autoantibodies that lead to synovial inflammation and eventual multi-joint destruction. A growing number of studies have shown significant differences in the gut microbiota composition of rheumatoid arthritis (RA) patients compared to healthy controls. Environmental factors, and changes in diet and nutrition are thought to play a role in developing this dysbiosis. This review aims to summarize the current knowledge of intestinal dysbiosis, the role of nutritional factors, and its implications in the pathogenesis of rheumatoid arthritis and autoimmunity. The future direction focuses on developing microbiome manipulation therapeutics for RA disease management.
Regulatory Roles of Noncoding RNAs in the Progression of Gastrointestinal Cancers and Health Disparities
Authors: Kulkarni Aditi , Gayathrinathan Sharan , Nair Soumya , Basu Anamika , Al-Hilal Taslim A. , Roy Sourav
Published: August 2022

Annually, more than a million individuals are diagnosed with gastrointestinal (GI) cancers worldwide. With the advancements in radio- and chemotherapy and surgery, the survival rates for GI cancer patients have improved in recent years. However, the prognosis for advanced-stage GI cancers remains poor. Site-specific GI cancers share a few common risk factors; however, they are largely distinct in their etiologies and descriptive epidemiologic profiles. A large number of mutations or copy number changes associated with carcinogenesis are commonly found in noncoding DNA regions, which transcribe several noncoding RNAs (ncRNAs) that are implicated to regulate cancer initiation, metastasis, and drug resistance. In this review, we summarize the regulatory functions of ncRNAs in GI cancer development, progression, chemoresistance, and health disparities. We also highlight the potential roles of ncRNAs as therapeutic targets and biomarkers, mainly focusing on their ethnicity-/race-specific prognostic value, and discuss the prospects of genome-wide association studies (GWAS) to investigate the contribution of ncRNAs in GI tumorigenesis.
Interplay of Developmental Hippo–Notch Signaling Pathways with the DNA Damage Response in Prostate Cancer
Authors: Mourkioti Ioanna , Angelopoulou Andriani , Belogiannis Konstantinos , Lagopati Nefeli , Potamianos Spyridon , Kyrodimos Efthymios , Gorgoulis Vassilis , Papaspyropoulos Angelos
Published: August 2022

Prostate cancer belongs in the class of hormone-dependent cancers, representing a major cause of cancer incidence in men worldwide. Since upon disease onset almost all prostate cancers are androgen-dependent and require active androgen receptor (AR) signaling for their survival, the primary treatment approach has for decades relied on inhibition of the AR pathway via androgen deprivation therapy (ADT). However, following this line of treatment, cancer cell pools often become resistant to therapy, contributing to disease progression towards the significantly more aggressive castration-resistant prostate cancer (CRPC) form, characterized by poor prognosis. It is, therefore, of critical importance to elucidate the molecular mechanisms and signaling pathways underlying the progression of early-stage prostate cancer towards CRPC. In this review, we aim to shed light on the role of major signaling pathways including the DNA damage response (DDR) and the developmental Hippo and Notch pathways in prostate tumorigenesis. We recapitulate key evidence demonstrating the crosstalk of those pathways as well as with pivotal prostate cancer-related ‘hubs’ such as AR signaling, and evaluate the clinical impact of those interactions. Moreover, we attempt to identify molecules of the complex DDR–Hippo–Notch interplay comprising potentially novel therapeutic targets in the battle against prostate tumorigenesis.
Smartphone-Based Quantitative Analysis of Protein Array Signals for Biomarker Detection in Lupus
Authors: Yang Guang , Li Yaxi , Tang Chenling , Lin Feng , Wu Tianfu , Bao Jiming
Published: August 2022

Fluorescence-based microarray offers great potential in clinical diagnostics due to its high-throughput capability, multiplex capabilities, and requirement for a minimal volume of precious clinical samples. However, the technique relies on expensive and complex imaging systems for the analysis of signals. In the present study, we developed a smartphone-based application to analyze signals from protein microarrays to quantify disease biomarkers. The application adopted Android Studio open platform for its wide access to smartphones, and Python was used to design a graphical user interface with fast data processing. The application provides multiple user functions such as “Read”, “Analyze”, “Calculate” and “Report”. For rapid and accurate results, we used ImageJ, Otsu thresholding, and local thresholding to quantify the fluorescent intensity of spots on the microarray. To verify the efficacy of the application, three antigens each with over 110 fluorescent spots were tested. Particularly, a positive correlation of over 0.97 was achieved when using this analytical tool compared to a standard test for detecting a potential biomarker in lupus nephritis. Collectively, this smartphone application tool shows promise for cheap, efficient, and portable on-site detection in point-of-care diagnostics.