bluuebunny/crossref_metadata_2025 huggingface dataset:
Genotype Diversity and Spread of White Spot Syndrome Virus (WSSV) in Madagascar (2012–2016)
Authors: Onihary Alain Moïse , Razanajatovo Iony Manitra , Rabetafika Lydia , Bastaraud Alexandra , Heraud Jean-Michel , Rasolofo Voahangy
Published: September 2021

White Spot Disease (WSD) caused by the White Spot Syndrome Virus (WSSV) is the most devastating viral disease threatening the shrimp culture industry worldwide, including Madagascar. WDS was first reported on the island in 2012; however, little is known about the circulation of the virus and its genetic diversity. Our study aimed at describing the molecular diversity and the spread of WSSV in the populations of Madagascan crustaceans. Farmed and wild shrimps were collected from various locations in Madagascar from 2012 to 2016 and were tested for WSSV. Amplicons from positive specimens targeting five molecular markers (ORF75, ORF94, ORF125, VR14/15 and VR23/24) were sequenced for genotyping characterizations. Four genotypes were found in Madagascar. The type-I genotype was observed in the south-west of Madagascar in April 2012, causing a disastrous epidemic, then spread to the North-West coast. Type-II strains were detected in October 2012 causing an outbreak in another Penaeus monodon farm. In 2014 and 2015, types II and III were observed in shrimp farms. Finally, in 2016, types II and IV were found in wild species including Fenneropenaeus indicus, Metapenaeus monoceros, Marsupenaeus japonicus and Macrobrachium rosenbergii. Considering the economic importance of the shrimp industry for Madagascar, our study highlights the need to maintain WSSV surveillance to quickly take appropriate countermeasures in case of outbreak and to sustain this industry.
Virological and Epidemiological Features of Norovirus Infections in Brazil, 2017–2018
Authors: Sarmento Sylvia Kahwage , de Andrade Juliana da Silva Ribeiro , Miagostovich Marize Pereira , Fumian Tulio Machado
Published: September 2021

Noroviruses are considered an important cause of acute gastroenteritis (AGE) across all age groups. Here, we investigated the incidence of norovirus, genotypes circulation, and norovirus shedding in AGE stool samples from outpatients in Brazil. During a two-year period, 1546 AGE stool samples from ten Brazilian states were analyzed by RT-qPCR to detect and quantify GI and GII noroviruses. Positive samples were genotyped by dual sequencing using the ORF1/2 junction region. Overall, we detected norovirus in 32.1% of samples, with a massive predominance of GII viruses (89.1%). We also observed a significant difference between the median viral load of norovirus GI (3.4×105 GC/g of stool) and GII (1.9×107 GC/g). The most affected age group was children aged between 6 and 24 m old, and norovirus infection was detected throughout the year without marked seasonality. Phylogenetic analysis of partial RdRp and VP1 regions identified six and 11 genotype combinations of GI and GII, respectively. GII.4 Sydney[P16] was by far the predominant genotype (47.6%), followed by GII.2[P16], GII.4 Sydney[P31], and GII.6[P7]. We detected, for the first time in Brazil, the intergenogroup recombinant genotype GIX.1[GII.P15]. Our study contributes to the knowledge of norovirus genotypes circulation at the national level, reinforcing the importance of molecular surveillance programs for future vaccine designs.
Examining the Effects of an Anti-Salmonella Bacteriophage Preparation, BAFASAL®, on Ex-Vivo Human Gut Microbiome Composition and Function Using a Multi-Omics Approach
Authors: Mayne Janice , Zhang Xu , Butcher James , Walker Krystal , Ning Zhibin , Wójcik Ewelina , Dastych Jarosław , Stintzi Alain , Figeys Daniel
Published: September 2021

Salmonella infections (salmonellosis) pose serious health risks to humans, usually via food-chain contamination. This foodborne pathogen causes major food losses and human illnesses, with significant economic impacts. Overuse of antibiotics in the food industry has led to multidrug-resistant strains of bacteria, and governments are now restricting their use, leading the food industry to search for alternatives to secure food chains. Bacteriophages, viruses that infect and kill bacteria, are currently being investigated and used as replacement treatments and prophylactics due to their specificity and efficacy. They are generally regarded as safe alternatives to antibiotics, as they are natural components of the ecosystem. However, when specifically used in the industry, they can also make their way into humans through our food chain or exposure, as is the case for antibiotics. In particular, agricultural workers could be repeatedly exposed to bacteriophages supplemented to animal feeds. To our knowledge, no studies have investigated the effects of such exposure to bacteriophages on the human gut microbiome. In this study, we used a novel in-vitro assay called RapidAIM to investigate the effect of a bacteriophage mixture, BAFASAL®, used in poultry farming on five individual human gut microbiomes. Multi-omics analyses, including 16S rRNA gene sequencing and metaproteomic, revealed that ex-vivo human gut microbiota composition and function were unaffected by BAFASAL® treatment, providing an additional measure for its safety. Due to the critical role of the gut microbiome in human health and the known role of bacteriophages in regulation of microbiome composition and function, we suggest assaying the impact of bacteriophage-cocktails on the human gut microbiome as a part of their safety assessment.
Structural Analysis of the Menangle Virus P Protein Reveals a Soft Boundary between Ordered and Disordered Regions
Authors: Webby Melissa N. , Herr Nicole , Bulloch Esther M. M. , Schmitz Michael , Keown Jeremy R. , Goldstone David C. , Kingston Richard L.
Published: September 2021

The paramyxoviral phosphoprotein (P protein) is the non-catalytic subunit of the viral RNA polymerase, and coordinates many of the molecular interactions required for RNA synthesis. All paramyxoviral P proteins oligomerize via a centrally located coiled-coil that is connected to a downstream binding domain by a dynamic linker. The C-terminal region of the P protein coordinates interactions between the catalytic subunit of the polymerase, and the viral nucleocapsid housing the genomic RNA. The inherent flexibility of the linker is believed to facilitate polymerase translocation. Here we report biophysical and structural characterization of the C-terminal region of the P protein from Menangle virus (MenV), a bat-borne paramyxovirus with zoonotic potential. The MenV P protein is tetrameric but can dissociate into dimers at sub-micromolar protein concentrations. The linker is globally disordered and can be modeled effectively as a worm-like chain. However, NMR analysis suggests very weak local preferences for alpha-helical and extended beta conformation exist within the linker. At the interface between the disordered linker and the structured C-terminal binding domain, a gradual disorder-to-order transition occurs, with X-ray crystallographic analysis revealing a dynamic interfacial structure that wraps the surface of the binding domain.
DNA Helicase–Polymerase Coupling in Bacteriophage DNA Replication
Authors: Lo Chen-Yu , Gao Yang
Published: September 2021

Bacteriophages have long been model systems to study the molecular mechanisms of DNA replication. During DNA replication, a DNA helicase and a DNA polymerase cooperatively unwind the parental DNA. By surveying recent data from three bacteriophage replication systems, we summarized the mechanistic basis of DNA replication by helicases and polymerases. Kinetic data have suggested that a polymerase or a helicase alone is a passive motor that is sensitive to the base-pairing energy of the DNA. When coupled together, the helicase–polymerase complex is able to unwind DNA actively. In bacteriophage T7, helicase and polymerase reside right at the replication fork where the parental DNA is separated into two daughter strands. The two motors pull the two daughter strands to opposite directions, while the polymerase provides a separation pin to split the fork. Although independently evolved and containing different replisome components, bacteriophage T4 replisome shares mechanistic features of Hel–Pol coupling that are similar to T7. Interestingly, in bacteriophages with a limited size of genome like Φ29, DNA polymerase itself can form a tunnel-like structure, which encircles the DNA template strand and facilitates strand displacement synthesis in the absence of a helicase. Studies on bacteriophage replication provide implications for the more complicated replication systems in bacteria, archaeal, and eukaryotic systems, as well as the RNA genome replication in RNA viruses.
In Situ Cancer Vaccination and Immunovirotherapy Using Oncolytic HSV
Authors: Jahan Nusrat , Ghouse Shanawaz M. , Martuza Robert L. , Rabkin Samuel D.
Published: September 2021

Herpes simplex virus (HSV) can be genetically altered to acquire oncolytic properties so that oncolytic HSV (oHSV) preferentially replicates in and kills cancer cells, while sparing normal cells, and inducing anti-tumor immune responses. Over the last three decades, a better understanding of HSV genes and functions, and improved genetic-engineering techniques led to the development of oHSV as a novel immunovirotherapy. The concept of in situ cancer vaccination (ISCV) was first introduced when oHSV was found to induce a specific systemic anti-tumor immune response with an abscopal effect on non-injected tumors, in the process of directly killing tumor cells. Thus, the use of oHSV for tumor vaccination in situ is antigen-agnostic. The research and development of oHSVs have moved rapidly, with the field of oncolytic viruses invigorated by the FDA/EMA approval of oHSV talimogene laherparepvec in 2015 for the treatment of advanced melanoma. Immunovirotherapy can be enhanced by arming oHSV with immunomodulatory transgenes and/or using them in combination with other chemotherapeutic and immunotherapeutic agents. This review offers an overview of the development of oHSV as an agent for ISCV against solid tumors, describing the multitude of different oHSVs and their efficacy in immunocompetent mouse models and in clinical trials.
Alterations in Brain Cannabinoid Receptor Levels Are Associated with HIV-Associated Neurocognitive Disorders in the ART Era: Implications for Therapeutic Strategies Targeting the Endocannabinoid System
Authors: Swinton Mary K. , Sundermann Erin E. , Pedersen Lauren , Nguyen Jacques D. , Grelotti David J. , Taffe Michael A. , Iudicello Jennifer E. , Fields Jerel Adam
Published: September 2021

HIV-associated neurocognitive disorders (HAND) persist despite the advent of antiretroviral therapy (ART), suggesting underlying systemic and central nervous system (CNS) inflammatory mechanisms. The endogenous cannabinoid receptors 1 and 2 (CB1 and CB2) modulate inflammatory gene expression and play an important role in maintaining neuronal homeostasis. Cannabis use is disproportionately high among people with HIV (PWH) and may provide a neuroprotective effect for those on ART due to its anti-inflammatory properties. However, expression profiles of CB1 and CB2 in the brains of PWH on ART with HAND have not been reported. In this study, biochemical and immunohistochemical analyses were performed to determine CB1 and CB2 expression in the brain specimens of HAND donors. Immunoblot revealed that CB1 and CB2 were differentially expressed in the frontal cortices of HAND brains compared to neurocognitively unimpaired (NUI) brains of PWH. CB1 expression levels negatively correlated with memory and information processing speed. CB1 was primarily localized to neuronal soma in HAND brains versus a more punctate distribution of neuronal processes in NUI brains. CB1 expression was increased in cells with glial morphology and showed increased colocalization with an astroglial marker. These results suggest that targeting the endocannabinoid system may be a potential therapeutic strategy for HAND.
Breakthrough Infections with Multiple Lineages of SARS-CoV-2 Variants Reveals Continued Risk of Severe Disease in Immunosuppressed Patients
Authors: Deng Xufang , Evdokimova Monika , O’Brien Amornrat , Rowe Cynthia L. , Clark Nina M. , Harrington Amanda , Reid Gail E. , Uprichard Susan L. , Baker Susan C.
Published: September 2021

The pandemic of COVID-19 caused by SARS-CoV-2 infection continues to spread around the world. Vaccines that elicit protective immunity have reduced infection and mortality, however new viral variants are arising that may evade vaccine-induced immunity or cause disease in individuals who are unable to develop robust vaccine-induced responses. Investigating the role of viral variants in causing severe disease, evading vaccine-elicited immunity, and infecting vulnerable individuals is important for developing strategies to control the pandemic. Here, we report fourteen breakthrough infections of SARS-CoV-2 in vaccinated individuals with symptoms ranging from asymptomatic/mild (6/14) to severe disease (8/14). High viral loads with a median Ct value of 19.6 were detected in the nasopharyngeal specimens from subjects regardless of disease severity. Sequence analysis revealed four distinct virus lineages, including alpha and gamma variants of concern. Immunosuppressed individuals were more likely to be hospitalized after infection (p = 0.047), however no specific variant was associated with severe disease. Our results highlight the high viral load that can occur in asymptomatic breakthrough infections and the vulnerability of immunosuppressed individuals to post-vaccination infections by diverse variants of SARS-CoV-2.
A Novel Recombinant Newcastle Disease Vaccine Improves Post- In Ovo Vaccination Survival with Sustained Protection against Virulent Challenge
Authors: Marcano Valerie C. , Cardenas-Garcia Stivalis , Diel Diego G. , Antoniassi da Silva Luciana H. , Gogal Robert M. , Miller Patti J. , Brown Corrie C. , Butt Salman Latif , Goraichuk Iryna V. , Dimitrov Kiril M. , Taylor Tonya L. , Williams-Coplin Dawn , Olivier Timothy L. , Stanton James B. , Afonso Claudio L.
Published: September 2021

In ovo vaccination has been employed by the poultry industry for over 20 years to control numerous avian diseases. Unfortunately, in ovo live vaccines against Newcastle disease have significant limitations, including high embryo mortality and the inability to induce full protection during the first two weeks of life. In this study, a recombinant live attenuated Newcastle disease virus vaccine containing the antisense sequence of chicken interleukin 4 (IL-4), rZJ1*L-IL4R, was used. The rZJ1*L-IL4R vaccine was administered in ovo to naïve specific pathogen free embryonated chicken eggs (ECEs) and evaluated against a homologous challenge. Controls included a live attenuated recombinant genotype VII vaccine based on the virus ZJ1 (rZJ1*L) backbone, the LaSota vaccine and diluent alone. In the first of two experiments, ECEs were vaccinated at 18 days of embryonation (DOE) with either 104.5 or 103.5 50% embryo infectious dose (EID50/egg) and chickens were challenged at 21 days post-hatch (DPH). In the second experiment, 103.5 EID50/egg of each vaccine was administered at 19 DOE, and chickens were challenged at 14 DPH. Chickens vaccinated with 103.5 EID50/egg of rZJ1*L-IL4R had hatch rates comparable to the group that received diluent alone, whereas other groups had significantly lower hatch rates. All vaccinated chickens survived challenge without displaying clinical disease, had protective hemagglutination inhibition titers, and shed comparable levels of challenge virus. The recombinant rZJ1*L-IL4R vaccine yielded lower post-vaccination mortality rates compared with the other in ovo NDV live vaccine candidates as well as provided strong protection post-challenge.
Descriptive Observational Study of Tdap Vaccination Adhesion in Pregnant Women in the Florentine Area (Tuscany, Italy) in 2019 and 2020
Authors: Bonito Benedetta , Balzi Daniela , Boccalini Sara , Bonanni Paolo , Mereu Giovanna , Santini Maria Grazia , Bechini Angela
Published: September 2021

Background: Tdap (Tetanus-Diphtheria-acellular Pertussis) vaccination is nowadays a worldwide-recommended practice to immunize pregnant women. The vaccine administration at the third trimester of pregnancy (as recommended by the WHO) would ensure antibody protection to both the mother and the newborn and has contributed to the significant drop of pertussis cases in infants. The aim of this observational study was to describe for the first time the socio-demographic characteristics and determinants of Tdap vaccination adhesion of pregnant women in the Florentine area. Methods: Information about parents’ vaccination status, their citizenship, employment type and mothers’ previous pregnancies and/or abortions were collected at the time of birth through the assistance birth certificates (CedAP) both for the years 2019 and 2020. This archive and the regional SISPC (Collective Prevention Healthcare Information System) linked using an anonymous unique personal identifier to retrieve the mother’s vaccination status. Results: We found an overall Tdap vaccination adhesion of 43% in 2019 and 47.3% in 2020. Several socio-demographic parameters would determine an increased vaccination adhesion, including parents’ geographical origin, mothers’ age and educational background, as well as the number of previous deliveries, abortions or voluntary termination of pregnancy. Conclusions: Since not much data are available on this topic in Italy, this study may constitute the baseline information for Tdap vaccination adhesion in pregnant women in the Florentine area (Italy). Thus, future successful vaccination strategies may be designed accordingly.
Enhancement of the HIV-1-Specific Immune Response Induced by an mRNA Vaccine through Boosting with a Poxvirus MVA Vector Expressing the Same Antigen
Authors: Gómez Carmen Elena , Perdiguero Beatriz , Usero Lorena , Marcos-Villar Laura , Miralles Laia , Leal Lorna , Sorzano Carlos Óscar S. , Sánchez-Corzo Cristina , Plana Montserrat , García Felipe , Esteban Mariano
Published: September 2021

Development of a vaccine against HIV remains a major target goal in the field. The recent success of mRNA vaccines against the coronavirus SARS-CoV-2 is pointing out a new era of vaccine designs against pathogens. Here, we have generated two types of mRNA vaccine candidates against HIV-1; one based on unmodified vectors and the other on 1-methyl-3′-pseudouridylyl modified vectors expressing a T cell multiepitopic construct including protective conserved epitopes from HIV-1 Gag, Pol and Nef proteins (referred to as RNA-TMEP and RNA-TMEPmod, respectively) and defined their biological and immunological properties in cultured cells and in mice. In cultured cells, both mRNA vectors expressed the corresponding protein, with higher levels observed in the unmodified mRNA, leading to activated macrophages with differential induction of innate immune molecules. In mice, intranodal administration of the mRNAs induced the activation of specific T cell (CD4 and CD8) responses, and the levels were markedly enhanced after a booster immunization with the poxvirus vector MVA-TMEP expressing the same antigen. This immune activation was maintained even three months later. These findings revealed a potent combined immunization regimen able to enhance the HIV-1-specific immune responses induced by an mRNA vaccine that might be applicable to human vaccination programs with mRNA and MVA vectors.
Risk Factors for Herpes Zoster in Patients with Chronic Kidney Disease: A Case-Control Study
Authors: Li Zhenxing , Wang Qiao , Ma Jiahui , Li Zhi , Huang Dong , Huang Yuzhao , Zhou Haocheng
Published: September 2021

(1) Background: Chronic kidney disease (CKD) increases the susceptibility to the presence of herpes zoster (HZ). Little is known about the risk factors of HZ in CKD patients; (2) Methods: This is a case-control study. CKD patients diagnosed with HZ between January 2015 and June 2021 in a tertiary hospital were identified. One age- and gender- matched control was paired for each case, matched to the date of initial HZ diagnosis. Conditional multiple logistic regression was used to evaluate the risk factors associated with the presence of HZ; (3) Results: Forty-seven HZ patients and controls were identified. In general, about 73.40% (69 out of 94) patients were classified at IV to V stages of CKD. Immunosuppressive agents (p = 0.0012) and dialysis therapy (p = 0.021) were reported more frequently in the HZ cohort. Compared with the control group, the total white cell count and lymphocyte count were significantly lower in the HZ group (p value of 0.032 and 0.003, respectively). The conditional logistics regression model revealed that previous immunosuppressants administration (odds ratio: 10.861, 95% CI: 2.092~56.392, p = 0.005) and dialysis therapy (odds ratio: 3.293, 95% CI: 1.047~10.355, p = 0.041) were independent risk factors of HZ in the CKD population; (4) Conclusions: Dialysis and immunosuppressants therapy were associated with greater risk of HZ disease in CKD patients. Further guideline may highlight the necessity of zoster vaccine for patients with CKD, who undertake associated treatment.
Predictors of Willingness to Receive the COVID-19 Vaccine after Emergency Use Authorization: The Role of Coping Appraisal
Authors: Xiao Qianyi , Liu Xin , Wang Ruru , Mao Yimeng , Chen Hao , Li Xiaomei , Liu Xiaoxi , Dai Junming , Gao Junling , Fu Hua , Zheng Pinpin
Published: September 2021

The current study aims to identify psychosocial factors based on protection motivation theory (PMT) influencing Chinese adults’ willingness to receive the COVID-19 vaccine after the emergency use authorization of the New Coronavirus Inactivated Vaccine in China. A cross-sectional online survey was conducted among Chinese residents aged 18–59 years, and 2528 respondents from 31 provinces and autonomous regions were included in the current study. Based on PMT, threat appraisals and coping appraisals were measured. Hierarchical multiple regressions and multivariate logistic regressions were used to identify the relationships between the PMT constructs and vaccination willingness after other covariates were controlled for. A total of 1411 (55.8%) respondents reported being willing to receive the COVID-19 vaccine. The PMT model explained 26.6% (p < 0.001) of the variance in the vaccine willingness. The coping appraisals, including response efficacy, self-efficacy, and response costs, were significantly correlated with the willingness to receive the COVID-19 vaccine, and response efficacy was the strongest influencing factor (adjusted OR = 2.93, 95% CI: 2.42–3.54). In conclusion, the coping appraisals for vaccination, instead of threat appraisals regarding the pandemic itself, mainly influenced people’s willingness to get vaccinated after the emergency use authorization of the COVID-19 vaccine in China. These findings are helpful for developing education and interventions to promote vaccination willingness and enhance public health outcomes during a pandemic.
A Therapeutic Hepatitis B Virus DNA Vaccine Induces Specific Immune Responses in Mice and Non-Human Primates
Authors: De Pooter Dorien , Van Gulck Ellen , Chen Antony , Evans Claire F. , Neefs Jean-Marc , Horton Helen , Boden Daniel
Published: September 2021

Despite the availability of an effective prophylactic vaccine for more than 30 years, nearly 300 million people worldwide are chronically infected with the hepatitis B virus (HBV), leading to 1 death every 30 s mainly from viral hepatitis-related cirrhosis and liver cancer. Chronic HBV patients exhibit weak, transient, or dysfunctional CD8+ T-cell responses to HBV, which contrasts with high CD8+ T-cell responses seen for resolvers of acute HBV infection. Therefore, a therapeutic DNA vaccine was designed, expressing both HBV core and polymerase proteins, and was sequence optimized to ensure high protein expression and secretion. Although the vaccine, administered intramuscularly via electroporation, had no effect on plasma viral parameters in a mouse model of persistent HBV infection, it did induce robust HBV-specific immune responses in healthy and adeno-associated hepatitis B virus (AAV-HBV) infected mice as well as in healthy non-human primates.
Counties with Lower Insurance Coverage and Housing Problems Are Associated with Both Slower Vaccine Rollout and Higher COVID-19 Incidence
Authors: Donadio Gregory , Choudhary Mayank , Lindemer Emily , Pawlowski Colin , Soundararajan Venky
Published: September 2021

Equitable vaccination distribution is a priority for outcompeting the transmission of COVID-19. Here, the impact of demographic, socioeconomic, and environmental factors on county-level vaccination rates and COVID-19 incidence changes is assessed. In particular, using data from 3142 US counties with over 328 million individuals, correlations were computed between cumulative vaccination rate and change in COVID-19 incidence from 1 December 2020 to 6 June 2021, with 44 different demographic, environmental, and socioeconomic factors. This correlation analysis was also performed using multivariate linear regression to adjust for age as a potential confounding variable. These correlation analyses demonstrated that counties with high levels of uninsured individuals have significantly lower COVID-19 vaccination rates (Spearman correlation: −0.460, p-value: <0.001). In addition, severe housing problems and high housing costs were strongly correlated with increased COVID-19 incidence (Spearman correlations: 0.335, 0.314, p-values: <0.001, <0.001). This study shows that socioeconomic factors are strongly correlated to both COVID-19 vaccination rates and incidence rates, underscoring the need to improve COVID-19 vaccination campaigns in marginalized communities.
P80 Natural Essence Exerts Efficient Anti-HIV-1- as Well as Adjuvant Effects in DCs
Authors: Zaderer Viktoria , Posch Wilfried , Gstir Ronald , Filipek Przemyslaw A. , Bonn Günther K. , Aramwit Pornanong , Huber Lukas A. , Wilflingseder Doris
Published: September 2021

Dendritic cells (DCs), as well as complement, play a major role during human immunodeficiency virus 1 (HIV-1) entry and infection at mucosal sites. Together, DCs and complement are key points for understanding host defence against HIV-1 infection and for studying the impact of new drugs on the regulation of innate host-pathogen interactions and adaptive immunity. For this, we evaluated the antiviral effect of the P80 natural essence (Longan extract) on interactions of non- and complement-opsonized HIV-1 with DCs. In viability assays, we first illustrated the effects of P80 natural essence on DC function. We found that P80 concentrations above 1.5% caused increased cell death, while at concentrations between 0.5% and 1% the compound exerted efficient antiviral effects in DCs and illustrated an adjuvant effect regarding DC activation. DC maturation, as well as co-stimulatory capacity, were significantly improved by P80 natural essence via p38 MAPK phosphorylation in presence of the viral challenge independent of the opsonization pattern. These findings might be exploited for future therapeutic options to target DC subsets directly at mucosal sites by P80 natural essence and to block entry of both, non- and complement-opsonized HIV-1.
Plant-Expressed Receptor Binding Domain of the SARS-CoV-2 Spike Protein Elicits Humoral Immunity in Mice
Authors: Maharjan Puna Maya , Cheon Jinyeong , Jung Jiyun , Kim Haerim , Lee Jaewon , Song Minjeong , Jeong Gi Uk , Kwon Youngchan , Shim Byoungshik , Choe Sunghwa
Published: September 2021

The current 15-month coronavirus disease-19 (COVID-19) pandemic caused by SARS-CoV-2 has accounted for 3.77 million deaths and enormous worldwide social and economic losses. A high volume of vaccine production is urgently required to eliminate COVID-19. Inexpensive and robust production platforms will improve the distribution of vaccines to resource-limited countries. Plant species offer such platforms, particularly through the production of recombinant proteins to serve as immunogens. To achieve this goal, here we expressed the receptor binding domain (RBD) of the SARS-CoV-2 spike (S) protein in the glycoengineered-tobacco plant Nicotiana benthamiana to provide a candidate subunit vaccine. This recombinant RBD elicited humoral immunity in mice via induction of highly neutralizing antibodies. These findings provide a strong foundation to further advance the development of plant-expressed RBD antigens for use as an effective, safe, and inexpensive SARS-CoV-2 vaccine. Moreover, our study further highlights the utility of plant species for vaccine development.
EV Overnight Charging Strategy in Residential Sector: Case of Winter Season in Quebec
Authors: Cardenas Alben , Guzman Cristina , Martinez Wilmar
Published: September 2021

Electric Vehicle (EV) technologies offer a leading-edge solution for clean transportation and have evolved substantially in recent years. The growing market and policies of governments predict EV massive penetration shortly; however, their large deployment faces some resistances such as the high prices compared to Internal Combustion Engine (ICE) cars, the required infrastructure, the liability for novelty and standardisation. During winter periods of cold countries, since the use of heating systems increases, the peak power may produce stress to the grid. This fact, combined with EVs high penetration, during charging periods inside of high consumption hours might overload the network, becoming a threat to its stability. This article presents a framework to evaluate load shifting strategies to reschedule the EV charging to lower grid load periods. The undesirable “rebound” effect of load shifting strategies is confirmed, leading us to our EV local overnight charging strategy (EV-ONCS). Our strategy combines the forecast of residential demand using probabilistic distribution from historical consumption, prediction of the EV expected availability to charge and the charging strategy itself. EV-ONCS avoids demand rebound of classic methods and allows a peak-to-average ratio reduction demonstrating the relief for the grid with very low implementation cost.
Effects of Sunflower Hulls on Productive Performance, Digestibility Indices and Rumen Morphology of Growing Awassi Lambs Fed with Total Mixed Rations
Authors: Alharthi Abdualrahman Salem , Al-Baadani Hani Hassan , Al-Badwi Mohammed Abduh , Abdelrahman Mutassim Mohammed , Alhidary Ibrahim Abdullah , Khan Rifat Ullah
Published: September 2021

Forty-eight growing Awassi lambs were used in a 70-day trial to investigate the effects of different levels of dietary sunflower hulls (SFH) on growth, rumen morphology, fiber digestibility and meat characteristics of lambs. Animals were randomly allocated to 4 groups with 3 replicates of 4 lambs each. The diet was composed of total mixed ration (TMR) without SFH (control group), and the TMR diet supplemented with SFH at a level of 5% (SFH5), 10% (SFH10) and 15% (SFH15). Lambs in the treatment groups had greater BW changes (p = 0.04) and ADG (p = 0.04) than the lambs in the control group. Intake of dry matter, acid detergent fiber (ADF) and neutral detergent fiber (NDF) were also significantly (p < 0.05) higher in SFH15 compared to SFH10. Digestibility of ADL and empty stomach weight were also significantly (p < 0.05) higher in SFH10 and SFH15, respectively. Cooking loss, blood total cholesterol and total protein decreased significantly (p < 0.05) in SFH15. Ruminal lightness (L) and yellowness (b) also increased significantly (p < 0.05) in SFH15. We concluded that the TMR diet supplemented with up to 15% SFH improved weight gain, digestibility, meat cooking loss and rumen color in Awassi lambs.
New Insight into the Prevalence and Risk Factors for Three Distinct Hoof Conformation Traits in UK Commercial Sheep Flocks
Authors: Best Caroline , Roden Janet , Phillips Kate , Pyatt Alison , Behnke Malgorzata
Published: September 2021

Lameness in sheep continues to be a global health, welfare and economic concern. Damaged, misshapen or overgrown feet have the potential to cause lameness either directly, or indirectly. There is a lack of understanding of the predisposing factors for different hoof conformation traits in sheep. Our exploratory study aimed to investigate the prevalence of, and risk factors for, three distinct hoof conformation traits relating to the sole and heel, hoof wall, and hoof wall overgrowth. Feet of 400 ewes from four UK commercial sheep farms were inspected at four time points across 12 months. For each conformation trait, a four-point ordinal system was used to score each individual claw, and foot-level scores were calculated. We present 92.4% of foot-level observations to be affected by ≥1 conformation traits. Whilst hoof conformation traits were correlated to some degree, a unique set of sheep-, foot- and farm-level factors were associated with each distinct conformation trait. We provide, for the first time, key insight into the multifaceted and multifactorial aetiology of hoof conformation in sheep, building upon previous landmark studies. Our results inform hypotheses for future prospective studies investigating the risk factors for adverse hoof conformation in sheep.
Linear Control of a Nonlinear Equipment Mounting Link
Authors: Williams Darren , Tagihpour Javad , Haddad Khodaparast Hamed , Jiffri Shakir
Published: September 2021

The linear control of a nonlinear response is investigated in this paper, and a nonlinear model of the system is developed and validated. The design of the control system has been constrained based on a suggested application, wherein mass and expense are parameters to be kept to a minimum. Through these restrictions, the array of potential applications for the control system is widened. The structure is envisioned as a robot manipulator link, and the control system utilises piezoelectric elements as both sensors and actuators. A nonlinear response is induced in the structure, and the control system is employed to attenuate these vibrations which would be considered a nuisance in practical applications. The nonlinear model is developed based on Euler–Bernoulli beam theory, where unknown parameters are obtained through optimisation based on a comparison with experimentally obtained data. This updated nonlinear model is then compared with the experimental results as a method of empirical validation. This research offers both a solution to unwanted nonlinear vibrations in a system, where weight and cost are driving design factors, and a method to model the response of a flexible link under conditions which yield a nonlinear response.
Changes in Algal Plankton Composition and Physico-Chemical Variables in a Shallow Oxbow Lake
Authors: Yaqoob Majd Muwafaq , Berta Csaba , Szabó László József , Dévai György , Szabó Szilárd , Nagy Sándor Alex , Bácsi István , Simon Alexandra , Nagy János , Somlyai Imre , Ács Éva , Grigorszky István
Published: September 2021

In our work, we sought to answer whether we find differences among the various zones of an oxbow lake with different land uses based on physico-chemical variables and dominant algal plankton species. The two ends of the oxbow lake are bordered by settlements, and near them there are open water areas where fishing is the major utilization form. Between the two open water areas we find a protected area with a large aquatic plant coverage and two transition zones towards the open water areas. The oxbow lake receives periodic water replenishment only at one end from one of the open water areas. During summer—due to the lack of rain—the water of the oxbow lake is used for irrigation in the surrounding arable land, so the water level fluctuation can be significant in the riverbed. Our study was performed within a vegetation period of spring, early summer, mid-summer, and fall. In connection with the ecological classification of a smaller water body, studies on the physical and chemical properties of the water and the composition of the algal plankton are usually carried out in few places and relatively infrequently. The characteristics of a water body are also influenced by seasonal changes, which can be the changes in the extent of vegetation coverage, the way land is used and the possibility of water replenishment, to which the algal community usually responds with changes. Based on our study, it can be said that even in a relatively small water body, we found a large differences based on the chemical and physical properties of the water and the characteristic algal species. Open water zones, areas with large macrovegetation coverage, and the transition zones were separated from each other.
Groundwater Quality Issues and Challenges for Drinking and Irrigation Uses in Central Ganga Basin Dominated with Rice-Wheat Cropping System
Authors: Kumar Sumant , Kumar Manish , Chandola Veerendra Kumar , Kumar Vinod , Saini Ravi K. , Pant Neeraj , Kumari Nikul , Srivastava Ankur , Singh Surjeet , Singh Rajesh , Krishan Gopal , Induwar Shashi Poonam , Kumar Sudhir , Yadav Brijesh Kumar , Maurya Nityanand Singh , Chaudhary Anju
Published: September 2021

Increased population and increasing demands for food in the Indo-Gangetic plain are likely to exert pressure on fresh water due to rise in demand for drinking and irrigation water. The study focuses on Bhojpur district, Bihar located in the central Ganga basin, to assess the groundwater quality for drinking and irrigation purpose and discuss the issues and challenges. Groundwater is mostly utilized in the study area for drinking and irrigation purposes (major crops sown in the area are rice and wheat). There were around 45 groundwater samples collected across the study region in the pre-monsoon season (year 2019). The chemical analytical results show that Ca2+, Mg2+ and HCO3− ions are present in abundance in groundwater and governing the groundwater chemistry. Further analysis shows that 66%, 69% and 84% of the samples exceeded the acceptable limit of arsenic (As), Fe and Mn respectively and other trace metals (Cu, Zn, Pb, Cd) are within the permissible limit of drinking water as prescribed by Bureau of Indian Standard for drinking water. Generally, high As concentration has been found in the aquifer (depth ranges from 20 to 40 m below ground surface) located in proximity of river Ganga. For assessing the irrigation water quality, sodium adsorption ratio (SAR) values, residual sodium carbonate (RSC), Na%, permeability index (PI) and calcium alteration index (CAI) were calculated and found that almost all the samples are found to be in good to excellent category for irrigation purposes. The groundwater facie has been classified into Ca-Mg-HCO3 type.
Removal of Pharmaceuticals from Wastewater: Analysis of the Past and Present Global Research Activities
Authors: Leyva-Díaz Juan Carlos , Batlles-delaFuente Ana , Molina-Moreno Valentín , Sánchez Molina Jorge , Belmonte-Ureña Luis J.
Published: September 2021

Water pollution is a worldwide problem. Water consumption increases at a faster rate than population and this leads to a higher pollution rate. Sustainable Development Goals (SDG) include proposals aimed at ensuring the availability of clean water and its sustainable management (Goal 6), as well as the conservation and sustainable use of oceans and seas. The current trend consists in trying to reconcile economic growth with sustainability, avoiding the negative externalities for the environment generated by human activity. More specifically, the objective of this article is to present the evolution of the research regarding the removal of polluting pharmaceuticals that are discharged into wastewater. To do that, a bibliometric analysis of 2938 articles comprising the period 1979–2020 has been carried out. This analysis includes productivity indicators in the scientific field: journals, authors, research institutions and countries. In addition, keyword analysis allows the identification of four main axes of the research regarding the removal of pharmaceutical residues found in wastewater. The first group of articles is aimed at identifying the pharmaceuticals present in polluting effluents. The second and third groups of articles focus on presenting the procedures that enable the treatment of emerging contaminants, either from a biological point of view (second group) or a physicochemical point of view (third group). The fourth group refers to water quality and its possibilities to be reused. Finally, there is a growing trend of worldwide scientific publications, which justifies the importance of polluting residues management, especially those of pharmaceutical origin, in order to achieve a more sustainable society.
Developing the Food, Water, and Energy Nexus for Food and Energy Scenarios with the World Trade Model
Authors: Cazcarro Ignacio , Dilekli Naci
Published: September 2021

The food, energy, and water (FEW) nexus has gained increased attention, resulting in numerous studies on management approaches. Themes of resource use, and their subsequent scarcity and economic rents, which are within the application domain of the World Trade Model, are ripe for study, with the continuing development of forward- and backward-facing economic data. Scenarios of future food and energy demand, relating to supply chains, as well as direct and indirect resource uses, are modelled in this paper. While it is possible to generate a substantial number of economic and environmental scenarios, our focus is on the development of an overarching approach involving a range of scenarios. We intend to establish a benchmark of possibilities in the context of the debates surrounding the Paris Climate Agreement (COP21) and the Green New Deal. Our approach draws heavily from the existing literature on international agreements and targets, notably that of COP21, whose application we associate with the Shared Socioeconomic Pathway (SSP). Relevant factor uses and scarcity rent increases are found and localized, e.g., on the optimal qualities of water, minerals, and land. A clear policy implication is that, in all scenarios, processes of energy transition, raw material use reduction, and recycling must be strengthened.
A Framework for Addressing Circularity Challenges in Cities with Nature-Based Solutions
Authors: Langergraber Guenter , Castellar Joana A. C. , Pucher Bernhard , Baganz Gösta F. M. , Milosevic Dragan , Andreucci Maria-Beatrice , Kearney Katharina , Pineda-Martos Rocío , Atanasova Nataša
Published: September 2021

A novel framework is presented that aims to guide practitioners and decision makers toward a better understanding of the role of nature-based solutions (NBS) in the enhancement of resources management in cities, and the mainstreaming of NBS in the urban fabric. Existing frameworks describing the use of NBS to address urban challenges do not specifically consider circularity challenges. Thus, the new framework provides the following: (1) a comprehensive set of Urban Circularity Challenges (UCCs); (2) a set of more than fifty NBS units and NBS interventions thoroughly assessed in terms of their potential to address UCCs; and (3) an analysis of input and output resource streams, which are both required for and produced during operation of NBS. The new framework aims to facilitate the coupling of individual NBS units and NBS interventions with NBS that enable circular economy solutions.
Seismic Performance Assessment of Water Distribution Systems Based on Multi-Indexed Nodal Importance
Authors: Hou Benwei , Ma Xitao , Diao Kegong , Zhong Zilan , Wu Shan
Published: September 2021

Seismic performance assessment of water distribution systems (WDSs) based on hydraulic simulation is essential for resilience evaluation of WDSs under earthquake disasters. The assessment is mainly to determine how the water supply will be affected due to pipe breaks caused by the earthquake, with the water supply loss estimated based on the loss of supply to nodes. Existing research works usually use the average or overall performance metric of all user nodes as the system performance indicator without considering user nodes’ individual performance and criticality. This paper proposes a framework to evaluate the importance of user nodes considering post-earthquake rescue service and the seismic performance of individual user nodes in the WDS, which supports the pipeline renovation plan to improve the performance of critical user nodes. The importance of user nodes is evaluated by a multi-index model, including the indices for daily service, post-earthquake rescue service, and network topology influence of user nodes. These indices evaluate the importance of user nodes in terms of their roles for daily water service, emergent rescue service, and water transmission to other nodes, respectively. Fragility model of pipelines evaluates the earthquake-induced damages of the WDS, and the seismic performance assessment of the WDS system is performed by the hydraulic model of the WDS with pipeline damages. The proposed framework is implemented in an actual WDS; the results show that the importance classification to user nodes by multi-index approach can identify the critical user nodes for post-earthquake rescue service, which traditional methods may ignore. The importance classification and seismic performance of individual user nodes make it feasible to check the seismic performance of critical user nodes and formulate a targeted pipeline renovation plan to focus limited resources on critical user nodes.
Science and Binational Cooperation: Bidirectionality in the Transboundary Aquifer Assessment Program in the Arizona-Sonora Border Region
Authors: Petersen-Perlman Jacob D. , Albrecht Tamee R. , Tapia-Villaseñor Elia M. , Varady Robert G. , Megdal Sharon B.
Published: September 2021

Sharing scientific data and information is often cited within academic literature as an initial step of water cooperation, but the transfer of research findings into policy and practice is often slow and inconsistent. Certain attributes—including salience, credibility, and legitimacy of scientific information; iterative information production; and sociocultural factors—may influence how easily scientific information can be used in management and policymaking. However, transnationality usually complicates these sorts of interactions. Accordingly, we argue that the production of scientific information and transboundary water cooperation build upon each other bidirectionally, each informing and enhancing the other. We employ a case-study analysis of the Transboundary Aquifer Assessment Program (TAAP), a binational collaborative effort for scientific assessment of aquifers shared between Mexico and the United States. Here, information sharing was possible only by first completing a formal, jointly agreed-upon cooperative framework in 2009. This framework resulted in a collaborative science production process, suggesting that the relationship between sharing data and information and transboundary groundwater governance is iterative and self-reinforcing. In keeping with the publication of the TAAP’s first binational scientific report in 2016, we demonstrate the bidirectional relationship between science production and water governance in the TAAP and explore remaining challenges after scientific assessment.