bluuebunny/crossref_metadata_2025 huggingface dataset:
Demographic and Genetic Impact of the 1742–1743 Plague Epidemic in Córdoba, Argentina: A Bioanthropological Perspective
Authors: Villafañe Jorge Hugo
Published: February 2025

Background: This study investigates the demographic, genetic, and socioeconomic impact of the 1742–1743 plague epidemic on Córdoba, a key region within the Viceroyalty of Peru. The research focuses on the epidemic’s influence along the Royal Road (Camino Real), the main route connecting Buenos Aires and Lima, addressing a historically overlooked period with a multidimensional approach. Methods: Historical records of deaths, baptisms, and marriages from the Córdoba Archbishopric Archive were analyzed using techniques from historical demography and bioanthropology. Variables such as endogamy, exogamy, average marital distance (AMD), and consanguinity coefficients were evaluated. Results: The findings reveal a significant increase in endogamy (75–82%) and a peak exogamy index of 375 during the 1740–1744 cohort. The AMD rose to 705 km during the epidemic, indicating a shift toward marriages involving individuals from more distant regions. The total consanguinity coefficient (Ft) reached 0.00056, with the non-random component (Fn) driving this increase, suggesting heightened genetic isolation. Conclusions: The 1742–1743 plague epidemic intensified genetic isolation and consanguinity, potentially increasing homozygosity and the prevalence of recessive conditions. These changes highlight the epidemic’s long-term impact on Córdoba’s genetic diversity and demographic patterns within the colonial context.
Enzyme-Assisted Extraction of Proteins from Cauliflower and Broccoli Waste Leaves
Authors: Sedlar Tea , Pasković Igor , Dragojlović Danka , Popović Senka , Vidosavljević Strahinja , Goreta Ban Smiljana , Popović Ljiljana
Published: February 2025

This research presents a novel approach to protein extraction from cauliflower (CL) and broccoli (BL) waste leaves by using enzymatic pretreatment, demonstrating its effectiveness on protein yield. Enzymatic pretreatment (pH 4.5, 10 h, t = 35 °C), was performed using commercial enzyme preparations, Viscozyme® L and Vinozyme®, in three different enzyme-to-substrate ratios E/S (0.2%, 2.5%, and 4.8%) of each enzyme. Leaf proteins (LPs) were obtained with alkaline extraction at pH 10–11 and their isoelectric precipitation at pH 4, following the control sample (extraction without enzymes). Protein yield (%), which was used as a parameter to monitor enzymatic efficiency, demonstrated a direct correlation with the enzyme-to-substrate (E/S) ratio. The highest protein yields were obtained at an enzyme concentration of 4.8% for both cellulolytic and pectolytic enzyme preparations, yielding 14.90 ± 0.12% for CL and 29.88 ± 0.86% for BL. The obtained proteins were characterized by FTIR spectroscopy and SDS-PAGE electrophoresis, and these methods confirmed the enzymatic efficiency of protein isolation. Isolated LPs showed high protein content for CLP 4.8% (77.27 ± 0.14%) and BPL 4.8% (84.66 ± 0.51%), and an increase in total amino acids, while the content of essential amino acids was over 40%. Protein solubility was assessed, revealing significant improvements (p < 0.05) in LPs derived from CL and BL at the highest E/S ratio of 4.8%, compared to the control sample C0%. Specifically, the solubility of CLP reached 29.4 mg/mL at pH 11, while BLP achieved 36.4 mg/mL at pH 10. As a result, these leaf proteins not only meet nutritional demands but also open innovative avenues of research in food science and biotechnology.
A Promising Genotype of Lepidium sativum for Enhanced Yield and Agronomic Performances Under Optimal Growth Conditions
Authors: Mohammed Said , Tiku Ketemaw , Daniel Frehiwot
Published: February 2025

Lepidium sativum, commonly known as garden cress, is a notable traditional medicinal plant within the Brassicaceae family, widely recognized for its health benefits in the globe. This study aimed to identify the seed colour variant best suited to the climate of Ethiopia, specifically in the Ataye region, by examining various agronomic traits. We conducted a comprehensive analysis of two distinct seed colour variants (brown and light blue) following standard agronomic protocols. Results indicated that the brown seed variant outperformed the light blue variant across multiple agronomic traits, demonstrating a shorter time to 50% germination, flowering, and maturation. Statistically significant differences were observed in these parameters. Additionally, plants derived from brown seeds exhibited superior growth characteristics, including greater plant height, longer primary branch length, and a higher number of primary branches per plant compared to those from light blue seeds. Furthermore, the brown seed variant displayed increased productivity, with more pods and seeds per plant, a greater one thousand seed weight, and a higher overall yield. The only traits showing no significant differences were the number of secondary branches per plant and internode length. Overall, the brown seed variant demonstrated strong adaptability to the Ataye climatic conditions, excelling in yield per hectare as well as in the number of seeds and pods produced. Based on these findings, we recommend the cultivation of brown seed colour variants for optimal performance in Ataye region, Ethiopia.
Compositional and Machine Learning Tools to Model Plant Nutrition: Overview and Perspectives
Authors: Parent Léon Etienne
Published: February 2025

The ceteris paribus assumption that all features are equal except the one(s) being examined limits the reliability of nutrient diagnosis and fertilizer recommendations. The objective of this paper is to review machine learning (ML) and compositional data analysis (CoDa) tools to make nutrient management feature specific. The accuracy of the ML methods averaged 84% across the crops. The additive and orthogonal log ratios of CoDa reduce a D-parts soil composition to D-1 variables, alleviating redundancy in the predictive ML models. Using a Brazilian onion (Allium cepa) database, the combined CoDa and ML methods returned crop response patterns, allowing feature-specific fertilizer recommendations to be made. The centered log ratio (clr) diagnoses plant nutrients as a compositional nutrient diagnosis (CND). Using a Quebec database of vegetable crops, the mean variance of clr variables (VAR¯) allowed comparing total variance among species and growth stages. While clr is the summation of equally weighted dual log ratios, dual nutrient log ratios may show unequal importance regarding crop performance. The RReliefF scores, gain ratios or gini inequality coefficients can provide weighting coefficients for each dual log ratio. The widely contrasting coefficients of weighted log ratios (wlr) improved the accuracy of the ML models for a Quebec muck onion database. The ML models, VAR¯ and wlr, are advanced tools to improve the accuracy of nutrient diagnosis.
Post-Prime Football Player Valuations: Depreciation Difference Between the English Premier League and the Top European Leagues
Authors: Liu James
Published: February 2025

This study explores market value depreciation among soccer players across the top five European leagues, addressing a critical gap in the sports finance literature by focusing on post-prime valuation dynamics. Leveraging a dataset from the 2023/2024 season, player market values and attributes sourced from Transfermarkt and Sportmonks were analyzed using league-specific multilinear regression models. The findings reveal a consistent decline in market values beginning at age 27, with notable variation across leagues. The German Bundesliga demonstrates the steepest depreciation rates, suggesting shorter career peaks or distinct market dynamics. In contrast, the Italian Serie A and Spanish La Liga exhibit the slowest depreciation rates, preserving player value for older athletes longer than other leagues. The English Premier League and French Ligue 1 show moderate depreciation, with the Premier League’s decline closely aligning with Ligue 1 and diverging less from other leagues than traditionally assumed. These results challenge preconceived narratives about league-specific player valuations and offer empirical insights into the transfer market. By providing a nuanced understanding of depreciation trends, this study can inform strategic decisions for agents, managers, and clubs, particularly in optimizing contract negotiations, transfer strategies, and long-term asset management in professional football.
Binge Eating and Obesity Differentially Alter the Mesolimbic Endocannabinoid System in Rats
Authors: Schoukroun Florian , Herbeaux Karin , Andry Virginie , Goumon Yannick , Bourdy Romain , Befort Katia
Published: February 2025

Binge eating disorder (BED) is characterized by the rapid overconsumption of palatable food in a short amount of time, often leading to obesity. The endocannabinoid system (ECS), a system involved in palatable food intake, is highly expressed in reward-related brain regions and is involved in both obesity and BED. This study investigated differences in ECS expression between these conditions using male Wistar rats exposed to specific regimen over six weeks: a non-access group (NA) with a standard diet, a continuous access group (CA) with free-choice high-fat high-sugar (fcHFHS) diet modeling obesity, and an intermittent access group (IA) with intermittent fcHFHS access modeling BED. Food intake was measured, and brain tissues from the nucleus accumbens (NAc), dorsal striatum (DS), ventral tegmental area (VTA), and rostromedial tegmental nucleus (RMTg) were analyzed for ECS expression using qPCR and mass spectrometry. We identified differential ECS expression across palatable food access groups, with variations depending on the brain region (striatal or mesencephalic). Correlation analyses revealed ECS dysregulations dependent on the type (fat or sucrose) and quantity of palatable food consumed. Comparative network analysis revealed co-regulation patterns of ECS-related genes with specific signatures associated with each eating pattern, highlighting RMTg as a key region for future research in eating behavior.
Markers of Type 2 Inflammation and Immunosenescence Are Upregulated in Localized Scleroderma
Authors: Khoury Lauren , Prosty Connor , Ghazal Stephanie , Gabrielli Sofianne , Torok Kathryn S. , Osman Mohammed , Martinez-Jaramillo Elvis , Lefrançois Philippe , Netchiporouk Elena
Published: February 2025

Localized scleroderma (LS) is an autoimmune, fibrotic skin disease that is thought to be triggered by environmental factors. Recent evidence from systemic autoimmune diseases proposed that the induction of immunosenescence may link environmental triggers with autoimmunity development. We aimed to explore the inflammatory signature in juvenile LS and investigate the presence of DNA instability and immunosenescence using publicly available transcriptomic data. High-throughput RNA sequencing data from 28 juvenile LS and 10 healthy controls were analyzed. Unsupervised clustering, pathway analyses, cell-type enrichment, fusion analyses, and immunosenescence gene set enrichment were performed. IFN and Type 1/2/3 pathways were upregulated in clinically active and histologically inflammatory LS. Type 2 inflammatory signature in both inflammatory and fibrotic LS was demonstrated by enriched genes, pathways, and deconvolution analyses (eosinophils). Features of genotoxic stress signals manifesting as DNA instability genes, pathways, and fusion events as well as mitochondrial dysfunction were demonstrated for the first time in LS. Features of immunosenescence (e.g., the upregulation of pathways involved in T cell exhaustion, inhibitory receptors, and cellular senescence and the enrichment of senescent genes) were also confirmed in (active and inflammatory) LS. Immunosenescence and inflammaging may underlie the complex and heterogeneous nature of immune responses seen in LS and should be further studied.
A Combined Thermodynamic and Computational Study of Alkaline Earth Metal Cations Complexation by a Fluorescent Calix[4]arene Receptor
Authors: Usenik Andrea , Modrušan Matija , Leko Katarina , Borovec Jakov , Marinac Sven , Hok Lucija , Cindro Nikola , Vianello Robert , Horvat Gordan , Požar Josip , Hrenar Tomica , Tomišić Vladislav
Published: February 2025

Complexation of alkaline earth metal cations with fluorescent tertiary-amide lower-rim calix[4]arene derivative bearing two phenanthridine moieties was studied experimentally (UV spectrophotometry, fluorimetry, isothermal microcalorimetry, NMR spectroscopy) and computationally (classical molecular dynamics and DFT calculations) at 25 °C. The complexation reactions were studied in acetonitrile, methanol, and ethanol, whereby the solvent effect on cation-binding processes was particularly addressed. The complex stability constants and standard reaction thermodynamic quantities (Gibbs energies, enthalpies, and entropies) were determined. The receptor exhibited particularly high affinity towards alkaline earth metal cations in acetonitrile, with peak affinity for Ca2+. The stability of all complexes was significantly lower in ethanol and methanol, where the most stable complex was formed with Sr2+. The decrease in cation-binding abilities was a consequence of the differences in solvation of the reactants and products of the complexation reactions (involving inclusion of the solvent molecule in the calixarene cone), cation charge density, as well as the cation–ligand binding site compatibility. The reactions were enthalpically controlled in acetonitrile, whereas in methanol and ethanol, the binding processes were endothermic and thus entropy driven. The results of 1H NMR measurements, MD simulations, and DFT calculations provided an insight into the structure of the complexes and the corresponding adducts with solvent molecules, as well as the structural aspects behind the differences in complexation thermodynamics. Due to the significant increase in its fluorescence upon cation binding, the studied calixarene derivative was proven to be a promising luminescent sensor for alkaline earth metal cations.
2-{N-[ω-(1-Benzylpiperidin-4-yl)alkyl]amino}-6-[(prop-2-yn-1-yl)amino]pyridine-3,5-dicarbonitriles Showing High Affinity for σ1/2 Receptors
Authors: Deuther-Conrad Winnie , Schepmann Dirk , Iriepa Isabel , López-Muñoz Francisco , Chioua Mourad , Wünsch Bernhard , Samadi Abdelouahid , Marco-Contelles José
Published: February 2025

Sigma receptors (σRs) represent very attractive biological targets for the development of potential agents for the treatment of several neurological disorders. In the search for new small molecule drugs against neuropathic pain, we identified 2-{[2-(1-benzylpiperidin-4-yl)ethyl]amino}-6-[methyl(prop-2-yn-1-yl)amino]pyridine-3,5-dicarbonitrile (5) as a polyfunctionalized small pyridine with potent dual-target activities against acetylcholinesterase (AChE) (IC50 = 13 nM) and butyrylcholinesterase (BuChE) (IC50 = 3.1 µM), exhibiting high σ1R affinity (Ki(hσ1R) = 1.45 nM) and 290-fold selectivity over the σ2R subtype. These results are in good agreement with those found in the molecular modeling of compound 5. This is possibly due to the preferred combination in this molecule of a linker n = 2 connecting the N-Bn-piperidine motif to the C2 pyridine, without a phenyl group at C4, and a N-Me-substituted propargyl amine in the chain located at C6.
The Pivotal Role of LACTB in the Process of Cancer Development
Authors: Zhang Minghui , Wu Bowen , Gu Jinke
Published: February 2025

The mitochondrial serine β-lactamase-like protein LACTB has emerged as a critical regulator in cancer biology, distinguished by its unique structural and functional attributes. Defined by its conserved penicillin-binding proteins and β-lactamases (PBP-βLs) domain and SXXK catalytic motif, LACTB demonstrates properties distinct from its prokaryotic homologs, including the ability to polymerize into filaments. These structural characteristics enable LACTB to modulate mitochondrial organization and enzymatic activity, influencing lipid metabolism and indirectly affecting cellular proliferation. Importantly, the expression and functional roles of LACTB exhibit cancer-type-specific variation, underscoring its dual function as both a tumor suppressor and an oncogene. Decreased LACTB expression is associated with poor clinical outcomes in cancers such as breast cancer, lung cancer, and colorectal cancer, while specific mutations and regulatory mechanisms have been linked to its oncogenic activity in osteosarcoma and pancreatic adenocarcinoma. Mechanistically, LACTB regulates key processes in cancer progression, including mitochondrial dynamics, epithelial–mesenchymal transition (EMT), and cell death pathways. This duality highlights LACTB as a promising therapeutic target and underscores its relevance in advancing precision oncology strategies. This review provides a comprehensive analysis of expression level, structure–function relationships, and the diverse roles of LACTB in oncogenesis, underscoring its promise as a focal point for precision cancer therapies.
Skin Telocyte Secretome as Conditioned Medium Prevents Profibrotic Differentiation of Skin Fibroblasts into Myofibroblasts
Authors: Rosa Irene , Fioretto Bianca Saveria , Andreucci Elena , Biagioni Alessio , Romano Eloisa , Manetti Mirko
Published: February 2025

Telocytes (TCs) are distinctive cells widely localized in the stromal compartment of several human organs, including the skin. By means of their peculiar prolongations named telopodes, skin TCs are organized in networks interconnected with a variety of adjacent cells, being thus supposed to take part in skin homeostasis through both cell-to-cell contacts and the release of extracellular vesicles. A disarrangement/loss of the TC network was shown in human fibrotic skin as well as in the murine model of bleomycin-induced cutaneous fibrosis, but whether such TC alterations may represent just a consequence or a trigger of the fibrotic process still remains to be clarified. Thus, we investigated the effects of skin TC secretome as conditioned medium (TC-CM) on the transition of skin fibroblasts into myofibroblasts promoted by the master profibrotic cytokine transforming growth factor β1 (TGFβ1). Primary cultures of both adult human skin TCs and fibroblasts were obtained by means of immunomagnetic cell separation. Nanoparticle tracking analysis was carried out to measure extracellular vesicles in TC-CM. The combination of multiple morphological, gene/protein expression, and functional assessments demonstrated that TC-CM was able to significantly prevent TGFβ1-induced fibroblast-to-myofibroblast transition. TC-CM did not influence cell viability, while it effectively inhibited TGFβ1-induced fibroblast proliferation, migration, and morphological changes. Indeed, TC-CM was able to reduce TGFβ1-mediated skin fibroblast phenotypic and functional differentiation into myofibroblasts, as shown by a significant decrease in FAP, ACTA2, COL1A1, COL1A2, FN1, and CTGF gene expression, α-smooth muscle actin, N-cadherin, COL1A1, and FN-EDA protein levels, and collagen gel matrix contraction. Furthermore, TC-CM significantly lowered TGFβ1-mediated ERK1/2 signaling pathway activation. This in vitro study proves for the first time that TCs may play an important role in skin homeostasis through the prevention of fibroblast-to-myofibroblast transition via paracrine mechanisms and affords the necessary basis to investigate in the future the feasibility of TC secretome as an innovative antifibrotic therapeutic tool.
30 Years Since the Proposal of Exon Skipping Therapy for Duchenne Muscular Dystrophy and the Future of Pseudoexon Skipping
Authors: Matsuo Masafumi
Published: February 2025

Thirty years ago, in 1995, I proposed a fundamental treatment for Duchenne Muscular Dystrophy (DMD) using antisense oligonucleotides (ASOs) to induce exon skipping and restore dystrophin expression. DMD is a progressive and fatal muscular dystrophy, and the establishment of an effective therapy has been a pressing demand among patients worldwide. Exon-skipping therapy utilizing ASOs has garnered significant attention as one of the most promising treatments for DMD, stimulating global research and development efforts in ASO technology. Two decades later, in 2016, one ASO was conditionally approved by the U.S. FDA as the first DMD treatment. This review summarizes the current status and challenges of ASO-based exon-skipping therapies for DMD and explores the prospects of pseudoexon skipping using ASOs, which holds the potential for achieving a complete cure for DMD.
Selenoproteins: Zoom-In to Their Metal-Binding Properties in Neurodegenerative Diseases
Authors: Duță Carmen , Muscurel Corina , Dogaru Carmen Beatrice , Stoian Irina
Published: February 2025

Selenoproteins contain selenium (Se), which is included in the 21st proteinogenic amino acid selenocysteine (Sec). Selenium (Se) is an essential trace element that exerts its biological actions mainly through selenoproteins. Selenoproteins have crucial roles in maintaining healthy brain activity. At the same time, brain-function-associated selenoproteins may also be involved in neurodegenerative diseases, such as Alzheimer’s disease (AD) and Parkinson’s disease (PD). The selenoproteins GPx4 (glutathione peroxidase 4), GPx1 (glutathione peroxidase 1), SELENOP (selenoprotein P), SELENOK (selenoprotein K), SELENOS (selenoprotein S), SELENOW (selenoprotein W), and SELENOT (selenoprotein T) are highly expressed, specifically in AD-related brain regions being closely correlated to brain function. Only a few selenoproteins, mentioned above (especially SELENOP), can bind transition and heavy metals. Metal ion homeostasis accomplishes the vital physiological function of the brain. Dyshomeostasis of these metals induces and entertains neurodegenerative diseases. In this review, we described some of the proposed and established mechanisms underlying the actions and properties of the above-mentioned selenoproteins having the characteristic feature of binding transition or heavy metals.
Dissecting the Affinity of Neighboring Malvaceae Genera; Brachychiton and Sterculia Through Morphological, Anatomical, and Molecular Approaches
Authors: Hamada Fatma A. , Elkholy Doaa M. , Hamdy Rim , Zamzam Mohamed , El-Taher Ahmed M. , Jan Hammad Ahmad , Atia Nagwan H.
Published: February 2025

Morphological, anatomical, and molecular information facilitates the identification and inference of the relatedness of plant species. In this study, the macromorphological, micromorphological, and anatomical characteristics of nine species from the Brachychiton and Sterculia genera belonging to the Malvaceae family were examined by light and a scanning electron microscope. The study recorded 66 macromorphological, micromorphological, and anatomical characteristics, thus revealing important variations between the studied species in leaf morphology and anatomy. This included variations in leaf complexity, leaf arrangement (phyllotaxy), epidermal cell walls, and their sculpture, as well as in the types of glandular and non-glandular trichomes. The studied species were mostly conserved in shedding patterns, being evergreen only in one out of nine studied species. Similarly, eight species were petiolate. Conversely, leaf arrangement and leaf complexity characteristics were highly divergent among the studied species, though only one species, Sterculia foetida, had compound leaves. The differences in the studied features and the chloroplast genes MaturaseK (MatK) and ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcl) were exploited to deduce the relationship between the studied species. While the morphological and anatomical features demonstrated a close relationship between the studied intrageneric species, the DNA barcoding analysis proved very efficient in distinguishing the two neighboring genera. Collectively, the different clustering analyses suggest a close relatedness between Brachychiton acerifolius and B. australis, while only DNA-based clustering demonstrates cladistic monophyly of the Sterculia species. This study, therefore, provides a detailed description of various morphological and anatomical features important for the systematic studies within the Malvaceae family and highlights the value of incorporating morphological, anatomical, and molecular approaches for inferring the evolutionary relationship between closely related plant species.
Advancing Cybersecurity with Honeypots and Deception Strategies
Authors: Morić Zlatan , Dakić Vedran , Regvart Damir
Published: February 2025

Cybersecurity threats are becoming more intricate, requiring preemptive actions to safeguard digital assets. This paper examines the function of honeypots as critical instruments for threat detection, analysis, and mitigation. A novel methodology for comparative analysis of honeypots is presented, offering a systematic framework to assess their efficacy. Seven honeypot solutions, namely Dionaea, Cowrie, Honeyd, Kippo, Amun, Glastopf, and Thug, are analyzed, encompassing various categories, including SSH and HTTP honeypots. The solutions are assessed via simulated network attacks and comparative analyses based on established criteria, including detection range, reliability, scalability, and data integrity. Dionaea and Cowrie exhibited remarkable versatility and precision, whereas Honeyd revealed scalability benefits despite encountering data quality issues. The research emphasizes the smooth incorporation of honeypots with current security protocols, including firewalls and incident response strategies, while offering comprehensive insights into attackers’ tactics, techniques, and procedures (TTPs). Emerging trends are examined, such as incorporating machine learning for adaptive detection and creating cloud-based honeypots. Recommendations for optimizing honeypot deployment include strategic placement, comprehensive monitoring, and ongoing updates. This research provides a detailed framework for selecting and implementing honeypots customized to organizational requirements.
Studies Toward Persilylation of π-Cyclopentadienyl Complexes of Fe and Ru. Molecular Structures of [Fe(C5H5){C5(SiMe2H)5}], [Fe(C5H5){C5Br3(SiMe3)2}] and [Fe(C5H5){C5Br2(SiMe3)3}]
Authors: Bernhartzeder Stefanie , Blockhaus Tobias , Lang Markus , Sünkel Karlheinz
Published: February 2025

Increasing the number of SiMe3 substituents on a cyclopentadienyl ring has, in addition to a stabilizing effect of unusual coordination geometries and oxidation states, the effect of increasing the solubility in unpolar solvents and increasing the volatility. Starting from pentabromoferrocene and pentabromo(pentamethyl)ruthenocene, we could achieve the introduction of up to five silyl (SiMe2H or SiMe3) groups to give [Fe(C5H5){C5(SiMe2R)5}], R = H, Me, and [Ru(C5Me5){C5(SiMe2H)5}]. However, yields were very low, and nearly all intermediate steps afforded mixtures of similar silyl-substituted compounds, which were difficult to separate. The crystal structures of [Fe(C5H5){C5(SiMe2H)5}] (13a), [Fe(C5H5){C5Br3(SiMe3)2}] (4b), and [Fe(C5H5){C5Br2(SiMe3)3}] (8b) were determined.
Phylogenetics and Evolutionary Dynamics of Yunnan Acrididae Grasshoppers Inferred from 17 New Mitochondrial Genomes
Authors: Zhang Keyao , Song Jing , Lu Junhui , Zhao Lu , Deng Weian , Guan Delong , Mao Benyong
Published: February 2025

Yunnan Province, a global biodiversity hotspot, hosts a diverse array of Acrididae grasshoppers essential for ecosystem dynamics and agriculture. To elucidate the phylogenetic relationships and evolutionary history of this group, we sequenced and analyzed complete mitochondrial genomes from 17 Acrididae species endemic to Yunnan, with genome lengths ranging from 15,403 to 15,943 base pairs. These data were integrated with mitochondrial sequences from 46 additional species to construct comprehensive phylogenetic trees. The maximum-likelihood tree identified four major clades with robust support (bootstrap values > 90%), revealing significant lineage diversification during the Early Eocene (51.94 million years ago, Mya) and subsequent radiations in the Miocene (~20 Mya) and Pliocene (~5 Mya). These divergence times correlate with major geological events and climatic shifts in the region, such as the uplift of the Tibetan Plateau and the intensification of the Asian monsoon. Notably, several species within the Coptacrinae and Oxyinae subfamilies, particularly Pseudodotraulia cornuata and Spathosternum prasiniferum, were found to be polyphyletic, indicating the necessity for taxonomic revisions. Further cluster analyses of codon usage bias and genetic distance support these taxonomic revisions within Acrididae. This robust phylogenetic framework underpins conservation strategies aimed at preserving Yunnan’s rich grasshopper biodiversity and informs updates to their phylogeny.
Impact of Omega-3 Fatty Acids Supplementation Combined with Resistance Training on Muscle Mass, Neuromuscular and Physical Function in Older Adults: A Systematic Review and Meta-Analysis of Randomized Clinical Trials
Authors: Dam Daniel L. , Christensen Jon A. , Olsen Pia Ø. , Wilson Jason J. , Tully Mark A. , Buhl Sussi F. , Caserotti Paolo
Published: February 2025

Age-related declines in muscle mass, neuromuscular, and physical function can be mitigated by resistance training (RT). Omega-3 polyunsaturated fatty acids (Ω-3 PUFAs) supplementation has shown benefits in older adults. However, it remains unclear if combining Ω-3 PUFAs with RT is more effective than RT alone or with placebo. This systematic review and meta-analysis examined the effects in randomized controlled trials (RCTs) of Ω-3 PUFAs combined with RT compared to RT alone or placebo on muscle mass and function in healthy older adults (≥65 y). Databases such as PubMed, Embase, SPORTDiscus, and Web of Science were searched on 11 April 2024. No restriction on language or publication date was implemented. Mean differences (MDs) or standardized mean differences (SMDs) with 95% confidence intervals and pooled effects were calculated. Nine studies (n = 286, 54% men) met the inclusion criteria. The meta-analysis found no significant effect of Ω-3 PUFAs on muscle mass or neuromuscular function but a large effect on chair-rise performance. Potential impact of Ω-3 PUFAs dose, duration, or sex were not observed. Most studies had varying levels of bias, and none met recommended quality standards for investigating Ω-3 PUFAs, but findings suggest no clear advantage of combining Ω-3 PUFAs with RT.
Longitudinal Displacement for Left Ventricular Function Assessment
Authors: Leitman Marina , Tyomkin Vladimir
Published: February 2025

Background: Quantitative evaluation of myocardial function traditionally relies on parameters such as ejection fraction and strain. Strain, reflecting the relative change in the length of a myocardial segment over the cardiac cycle, has been extensively studied in various cardiac pathologies over the past two decades. However, the absolute length change, or longitudinal displacement, of myocardial segments during the cardiac cycle has received limited attention. This study aims to evaluate longitudinal displacement in two separate groups: healthy athletes and patients with left ventricular dysfunction, providing new insights into myocardial function assessment. Methods: Echocardiographic examinations were performed on 30 healthy football players and 30 patients with left ventricular dysfunction using speckle-tracking imaging analysis. Global and regional peak longitudinal displacement values were calculated and compared with corresponding global and regional peak longitudinal strain measurements. A manual alternative for calculating global longitudinal strain was also proposed. Results: An inverse correlation was found between regional longitudinal displacement and regional longitudinal strain. Longitudinal displacement was maximal in the basal segments and lowest in the apex of the left ventricle, exhibiting a reversed basal-to-apical gradient (17.6 ± 3.5 mm vs. 11.5 ± 2.9 mm vs. 4.22 ± 1.7 mm in basal, mid, and apical segments, respectively; p < 0.000001). Maximal longitudinal displacement was observed in the inferior and posterior walls of the left ventricle. In the 30 patients with left ventricular dysfunction, global longitudinal displacement was significantly lower than in healthy individuals (4.4 ± 1.7 mm vs. 11.7 ± 1.5 mm, p < 0.000001). Global longitudinal displacement and global longitudinal strain showed a strong negative correlation (r = −0.72, p < 0.000001). Manually calculated global longitudinal strain demonstrated good agreement with speckle-tracking-based global longitudinal strain. Conclusions: Peak longitudinal displacement can be used to evaluate both regional and global myocardial function, similarly to peak longitudinal strain. Unlike strain, longitudinal displacement exhibits a reversed basal-to-apical gradient, with the highest values at the base of the left ventricle and the lowest at the apex. Global and regional longitudinal displacement is significantly reduced in patients with left ventricular dysfunction. Global longitudinal strain can be manually calculated using displacement measurements. Further studies are needed to evaluate peak longitudinal displacement in various cardiac pathologies.
Antibacterial UV-Curable Gel with Hydroxyapatite Nanoparticles for Regenerative Medicine in the Field of Orthopedics
Authors: Burunkova Julia A. , Semykina Valeria V. , Sitnikova Vera E. , Dolgintsev Dmitry M. , Zaripova Faliya F. , Ponomareva Alina A. , Mizina Diana R. , Csick Attila , Kokenyesi Sandor , Zhilenkov Anton
Published: February 2025

The development and analysis of the properties of a new material based on UV-curable acrylate monomers with silicon-containing hydroxyapatite and zinc oxide nanoparticles as an antibacterial component and gelatin was carried out. Using this material in orthopedics and dentistry is very convenient because it covers any surface geometry of metal implants and hardens under ultraviolet light. In this work, sorption properties, changes in porosity, and mechanical properties of the material were investigated. The conditions for obtaining hydroxyapatite (HA) nanoparticles and the presence of silicon oxide nanoparticles and organic for the shell in an aqueous medium were studied for the pH of the medium, the sequence of administration and concentration of the material components, as well as antibacterial properties. This polymer material is partially resorbable. That supports not only the growth of bone cells but also serves as a protective layer. It reduces friction between organic tissues and a metal implant and can be a solution to the problem of the aseptic instability of metal implants. The material can also be used to repair damaged bones and cartilage tissues, especially in cases where the application and curing procedure is performed using laparoscopic methods. In this work, the authors propose a simple and quite cheap method for obtaining material based on photopolymerizable acrylates and natural gelatin with nanoparticles of HA, zinc oxide, and silicon oxide. The method allows one to obtain a composite material with different nanoparticles in a polymer matrix which retain the requisite properties needed such as active-sized HA, antibacterial ZnO, and structure-forming and stability-improving SiO2 nanoparticles.
Exploring the Properties and Indications of Chairside CAD/CAM Materials in Restorative Dentistry
Authors: Ille Codruţa-Eliza , Jivănescu Anca , Pop Daniel , Stoica Eniko Tunde , Flueras Razvan , Talpoş-Niculescu Ioana-Cristina , Cosoroabă Raluca Mioara , Popovici Ramona-Amina , Olariu Iustin
Published: February 2025

The present review provides an up-to-date overview of chairside CAD/CAM materials used in restorative dentistry, focusing on their classification, properties, and clinical applications. If CAD/CAM technology was only an aspiration in the past, a higher proportion of clinics are employing it nowadays. The market is overflowing with biomaterials, and these materials are constantly evolving, making it challenging for practitioners to choose the most appropriate one, especially in correlation with patients’ medical diseases. The evolution of CAD/CAM technology has revolutionized dental practice, enabling the efficient fabrication of high-quality restorations in a single appointment. The main categories of chairside CAD/CAM materials include feldspathic ceramics, leucite-reinforced ceramics, lithium disilicate, zirconia, hybrid ceramics, and acrylic resins. The mechanical, physical, and aesthetic properties of these materials are discussed, along with their advantages and limitations for different clinical scenarios. Factors influencing material selection, such as strength, aesthetics, and ease of use, are also assessed. Ultimately, the guiding principle of dentistry is minimally invasive treatment following the particularity of the clinical case to obtain the envisioned result. Correlating all these factors, a simple, up-to-date classification is required to begin an individualized treatment. By synthesizing current evidence, this comprehensive review aims to guide clinicians in selecting appropriate chairside CAD/CAM materials to achieve optimal functional and aesthetic outcomes in restorative procedures. The integration of digital workflows and continued development of novel materials promise to further enhance the capabilities of chairside CAD/CAM systems in modern dental practice.
Echinocandin Adaptation in Candida albicans Is Accompanied by Altered Chromatin Accessibility at Gene Promoters and by Cell Wall Remodeling
Authors: Sah Sudisht K. , Yadav Anshuman , Stahl Tyler , Hayes Jeffrey J. , Bulger Michael , Rustchenko Elena
Published: February 2025

Infections by the major opportunistic pathogen of human Candida albicans are commonly treated with echinocandin (ECN) drugs. However, C. albicans can adapt to grow in the presence of certain amounts of ECNs. Prior studies by several laboratories have defined multiple genes, as well as mechanisms involving induced aneuploidy, that can govern this. Still, the mechanisms of ECN adaptation are not fully understood. Here, we use genome-wide profiling of chromatin accessibility by ATAC-seq to determine if ECN adaptation is reflected in changes in the chromatin landscape in the absence of aneuploidy. We find that drug adaptation is coupled with multiple changes in chromatin accessibility genome-wide, which occur predominantly in gene promoter regions. Areas of increased accessibilities in promoters are enriched with the binding motifs for at least two types of transcription factors: zinc finger and basic leucine zipper. We also find that chromatin changes are often associated with differentially expressed genes including genes with functions relevant to the ECN-adapted phenotype, such as cell wall biosynthesis. Consistent with this, we find that the cell wall is remodeled in ECN-adapted mutants, with chitin up and glucan down and increased cell surface exposure. A full understanding of ECN adaptation processes is of critical importance for the prevention of clinical resistance.
New Standardized Procedure to Extract Glyphosate and Aminomethylphosphonic Acid from Different Matrices: A Kit for HPLC-UV Detection
Authors: Chiara Francesco , Allegra Sarah , Arrigo Elisa , Di Grazia Daniela , Shelton Agar Francesco Maximillian Anthony , Abalai Raluca Elena , Gilardi Sara , De Francia Silvia , Mancardi Daniele
Published: February 2025

Background: Glyphosate has been extensively used as herbicide since the early 1970s. The daily exposure limit is set at 0.3 mg/kg bw/d in Europe and 1.75 mg/kg bw/d in the USA. Among its derivatives, aminomethylphosphonic acid is the most stable and abundant. Understanding their biological effects then requires reliable methods for quantification in biological samples. Methods: We developed and validated a fast, low-cost, and reliable chromatographic method for determining glyphosate and aminomethylphosphonic acid concentrations. The validation included following parameters: specificity, selectivity, matrix effect, accuracy, precision, calibration performance, limit of quantification, recovery, and stability. Sample extraction employed an anion exchange resin with elution using hydrochloric acid 50.0 mmol/L. For HPLC analysis, analytes were derivatized, separated on a C18 column with a mobile phase of phosphate buffer (0.20 mol/L, pH 3.0) and acetonitrile (85:15), and detected at 240 nm. Results: The method demonstrated high reliability and reproducibility across various matrices. Its performance met all validation criteria, confirming its suitability for quantifying glyphosate and aminomethylphosphonic acid in different biological and experimental setups. Conclusions: This method can offer a practical resource for applications in experimental research, medical diagnostics, quality control, and food safety.
The Impact of Earnings Announcements Before and After Regular Market Hours on Asset Price Dynamics in the Fintech Era
Authors: Tripathi Janhavi Shankar , Rengifo Erick W.
Published: February 2025

With the recent increase in retail investor participation led by commission-less fintech trading applications and new features like fractional trading, we now have higher volatility and significantly quicker price changes. This makes it hard to make informed trading decisions. Moreover, these effects are exacerbated even further around earnings announcements days. In this paper, we use Nasdaq data feed at a minute frequency and show that there is a significant increase in the slope of the price–volume structure during extended hours (after-hours, or pre-market hours) as compared with the ones observed during regular market times. Our analysis shows that the liquidity is much less during the extended market hours. As such, earnings announcements of stocks during these times have a significantly larger price impact than those stocks that have their earnings announced during regular trading hours. This significant difference can be explained by observing the limit order book structures during these different trading periods. We suggest that the earnings announcements should not be made during extended hours given the significantly lower liquidity and thus, the significantly larger price impact that not only determines the prices for the next trading session but also sets the new “fundamental” price signals for the stocks.
The Stability of the Financial Cycle: Insights from a Markov Switching Regression in South Africa
Authors: Magubane Khwazi
Published: February 2025

The stability of the financial cycle is paramount for the effective formulation and implementation of macroprudential policy in South Africa. The South African Reserve Bank (SARB) and the Prudential Authority strive to mitigate excessive fluctuations in the financial cycle, recognising that a stable cycle provides more reliable signals for financial sector activity and anchors macroprudential policy decisions. However, the tightening of macroprudential policy by the SARB and the Prudential Authority during the post-2009 recovery period, despite mild signs of recovery from the global financial crisis, raises concerns about the stability of the South African financial cycle. This study aims to construct a financial cycle volatility index to assess its stability and identify the key macroeconomic drivers of financial instability in South Africa. Employing monthly data from 1970 to 2024, the study utilises a dynamic conditional correlation model and a Markov switching regression model to analyse the relationship between macroeconomic variables and financial stability. The findings reveal heightened financial cycle volatility around crisis periods and demonstrate that macroeconomic variables such as exchange rate fluctuations, price level changes, and implementing monetary and macroprudential policies can significantly increase financial instability. These results suggest a need for proactive and aggressive macroprudential policy measures in the years preceding potential crises. Moreover, the study’s findings emphasise the importance of considering macroeconomic conditions when calibrating financial cycle policies.
Financial Openness, Trade Openness, and Economic Growth Nexus: A Dynamic Panel Analysis for Emerging and Developing Economies
Authors: Seti Thembalethu Macdonald , Mazwane Sukoluhle , Christian Mzuyanda
Published: February 2025

International market openness has long been regarded as critical for economic development, and recent evidence highlights the distinct roles of financial and trade openness, particularly in emerging and developing economies. This study examines the impact of financial and trade openness on economic growth in ten emerging and developing countries from 1970 to 2023. It employs a dynamic panel generalized method of moments (GMM) model, which is selected for its ability to address potential endogeneity and dynamic relationships within panel data. The analysis finds that both financial and trade openness positively influence economic growth and that stable macroeconomic conditions and political stability enhance these growth-promoting effects. In the context of growing geo-economic tensions, trade fairness, and national security concerns, the study underscores the need for policies that balance global integration with national interests. These findings suggest the importance of designing policies that promote greater integration into global financial and trading systems while ensuring sound macroeconomic fundamentals and supportive institutions. The study recommends that policymakers pursue strategic liberalization and strengthen governance structures to achieve sustained and inclusive growth.
The Difficulties of Ex Situ Conservation: A Nationwide Investigation of Avian Haemosporidia Among Captive Penguins in Japan
Authors: Inumaru Mizue , Shimizu Misa , Shibata Ayumi , Murata Koichi , Sato Yukita
Published: February 2025

Avian malaria has been a continuous problem in both wild and captive populations of penguins throughout the world. In Japan, where there are over 3000 captive penguins, avian malaria (by Plasmodium spp.) and haemoproteosis (by Haemoproteus spp.) have been sporadically detected throughout the country. However, no comprehensive studies have been carried out, and the national status of infection has been unknown until now. In this study, the prevalence and lineage composition of haemosporidian parasites was investigated in captive penguins throughout Japan for the first time. A total of 1203 penguins from 55 facilities were sampled from January 2010 to December 2019. Parasites were detected by nested PCR and microscopy of blood and tissue samples. The total prevalence was 7.48% for Plasmodium and 1.75% for Haemoproteus, of which some are suggested to have been acquired during the study period. The odds of infection were higher in individuals kept outdoors compared to indoors, re-confirming that exposure to vectors is one of the major factors. Additionally, the odds of death were higher in infected individuals, although differences between parasite lineages were also observed. This study provides an overview of avian malaria in penguins of Japan in the hope of guiding future studies and conservation actions in captivity.