bluuebunny/crossref_metadata_2025 huggingface dataset:
Exposure Levels of Airborne Fungi, Bacteria, and Antibiotic Resistance Genes in Cotton Farms during Cotton Harvesting and Evaluations of N95 Respirators against These Bioaerosols
Authors: Adhikari Atin , Banerjee Pratik , Thornton Taylor , Jones Daleniece Higgins , Adeoye Caleb , Sherpa Sonam
Published: June 2023

The USA is the third-leading cotton-producing country worldwide and cotton farming is common in the state of Georgia. Cotton harvest can be a significant contributor to airborne microbial exposures to farmers and nearby rural communities. The use of respirators or masks is one of the viable options for reducing organic dust and bioaerosol exposures among farmers. Unfortunately, the OSHA Respiratory Protection Standard (29 CFR Part 1910.134) does not apply to agricultural workplaces and the filtration efficiency of N95 respirators was never field-tested against airborne microorganisms and antibiotic resistance genes (ARGs) during cotton harvesting. This study addressed these two information gaps. Airborne culturable microorganisms were sampled using an SAS Super 100 Air Sampler in three cotton farms during cotton harvesting, and colonies were counted and converted to airborne concentrations. Genomic DNA was extracted from air samples using a PowerSoil® DNA Isolation Kit. A series of comparative critical threshold (2−ΔΔCT) real-time PCR was used to quantify targeted bacterial (16S rRNA) genes and major ARGs. Two N95 facepiece respirator models (cup-shaped and pleated) were evaluated for their protection against culturable bacteria and fungi, total microbial load in terms of surface ATP levels, and ARGs using a field experimental setup. Overall, culturable microbial exposure levels ranged between 103 and 104 CFU/m3 during cotton harvesting, which was lower when compared with bioaerosol loads reported earlier during other types of grain harvesting. The findings suggested that cotton harvesting works can release antibiotic resistance genes in farm air and the highest abundance was observed for phenicol. Field experimental data suggested that tested N95 respirators did not provide desirable >95% protections against culturable microorganisms, the total microbial load, and ARGs during cotton harvesting.
Molecular Phylogenies of Leeches and Haemoparasites Infecting Freshwater Turtles in Aquatic Ecosystems of Northern Africa Suggest Phylogenetic Congruence between Placobdella costata Sensu Lato and Haemogregarina stepanowi Sensu Lato
Authors: Verneau Olivier , Melliti Sirine , Kimdil Latifa , El Mouden El Hassan , Achouri Mohamed Sghaier , Rouag Rachid
Published: June 2023

Haemogregarines are blood parasites with a life-cycle involving a vertebrate as the intermediate host and an invertebrate as the definitive host and vector. Extensive phylogenetic investigations based on 18S-rRNA gene sequences have shown that Haemogregarina stepanowi (Apicomplexa: Haemogregarinidae) is able to infest a large diversity of freshwater turtle species, including the European pond turtle Emys orbicularis, the Sicilian pond turtle Emys trinacris, the Caspian turtle Mauremys caspica, the Mediterranean pond turtle Mauremys leprosa, and the Western Caspian turtle Mauremys rivulata, among others. From the same molecular markers, H. stepanowi is further considered to be a complex of cryptic species predisposed to infect the same host species. While Placobdella costata is known to be the unique vector of H. stepanowi, it is only recently that independent lineages within P. costata have been illustrated—suggesting the presence of at least five unique leech species across Western Europe. The aims of our study were therefore to investigate from mitochondrial markers (COI) the genetic diversity within haemogregarines and leeches infecting freshwater turtles of the Maghreb, in order to identify processes of parasite speciation. We showed that H. stepanowi consists of at least five cryptic species in the Maghreb, while two Placobella species were identified in the same area. Although an Eastern–Western speciation pattern was apparent for both leeches and haemogregarines, we cannot make definitive conclusions regarding co-speciation patterns between parasites and vectors. However, we cannot reject the hypothesis of a very strict host–parasite specificity within leeches.
Severe Combined Immunodeficiency—Classification, Microbiology Association and Treatment
Authors: Justiz-Vaillant Angel A. , Gopaul Darren , Akpaka Patrick Eberechi , Soodeen Sachin , Arozarena Fundora Rodolfo
Published: June 2023

Severe combined immunodeficiency (SCID) is a primary inherited immunodeficiency disease that presents before the age of three months and can be fatal. It is usually due to opportunistic infections caused by bacteria, viruses, fungi, and protozoa resulting in a decrease in number and impairment in the function of T and B cells. Autosomal, X-linked, and sporadic forms exist. Evidence of recurrent opportunistic infections and lymphopenia very early in life should prompt immunological investigation and suspicion of this rare disorder. Adequate stem cell transplantation is the treatment of choice. This review aimed to provide a comprehensive approach to the microorganisms associated with severe combined immunodeficiency (SCID) and its management. We describe SCID as a syndrome and summarize the different microorganisms that affect children and how they can be investigated and treated.
Pathway to Prediction of Pyrite Floatability from Copper Ore Geological Domain Data
Authors: Yenial-Arslan Unzile , Jefferson Mayra , Curtis-Morar Catherine , Forbes Elizaveta
Published: June 2023

The depletion of mining resources forces the mining industry to process more heterogeneous and complex orebodies. The inherent heterogeneity of these orebodies and their relation to processing recoveries have received considerable interest in recent years. The properties of ores, such as mineral composition and association, are known to affect flotation performance. Even ores with similar compositions can vary significantly regarding their texture, where the same minerals can occur in different forms. Therefore, very careful geometallurgical planning is needed to overcome the recovery losses. Glencore’s Mount Isa Copper Operation has reported historical difficulties decreasing the copper losses associated with natural floatable pyrites. Understanding the rock properties of naturally floatable pyrites and how they relate to chalcopyrite losses is crucial for concentrator operations. The Mount Isa geometallurgy team is looking for proxies for predicting copper losses and natural floatable pyrites to improve mine planning. This paper presents an approach for predicting the collector-less flotation of pyrite, as well as chalcopyrite losses from rock properties. The statistical analysis between the rock quality and ore type gives an indication of the chalcopyrite losses and natural floatable pyrites, which has potential use in geometallurgy plans.
Preparation and Characterization of Asphalt Pitch-Derived Activated Carbons with Enhanced Electrochemical Performance as EDLC Electrode Materials
Authors: Kim Ju-Hwan , Kim Young-Jun , Kang Seok-Chang , Lee Hye-Min , Kim Byung-Joo
Published: June 2023

This study used a physical activation method to prepare asphalt-pitch-derived activated carbon (Pitch AC) for an electric double-layer capacitor (EDLC) electrode. X-ray diffraction analysis and Raman spectroscopy were used to estimate the change in the crystal structure of Pitch AC with activation time. In addition, the textural properties of Pitch AC were studied by Brunauer-Emmett-Teller (BET), Dubinin-Radushkevich (DR) and non-localized density functional theory (NLDFT) equations with N2/77K isotherm adsorption-desorption curves. The electrochemical performance of the Pitch AC was analyzed using a coin-type EDLC with 1 M SBPBF4/PC via galvanostatic charge/discharge, cyclic voltammetry and electrochemical impedance spectroscopy. The specific surface area and total pore volume were 990–2040 m2/g and 0.42–1.51 cm3/g, respectively. The pore characteristics of the Pitch AC varied according to the activation time and changed from a microporous structure to a micro-mesoporous structure as the activation time increased. The electrochemical performance analysis also found that the specific capacity was increased from 43.6 F/g to 84.5 F/g at 0.1 A/g as activation time increased. In particular, Pitch AC-9 exhibited the best electrochemical performance (rectangular CV curve, reversible GCD, lowest ion charge transfer resistance and Warburg impedance). In addition, Pitch AC-9 was confirmed to have a specific capacitance similar to commercial activated carbon for EDLC (YP-50F). Therefore, it was considered that Pitch AC could replace commercial activated carbon for EDLC because it has excellent pore characteristics and electrochemical performance despite being manufactured through a very low-cost precursor and a simple process (physical activation method).
DPPH Radical Scavenging Activity of New Phenolics from the Fermentation Broth of Mushroom Morehella importuna
Authors: Wang Feifei , Tan Jie , Jiang Ruixiang , Li Feifei , Zheng Renqing , Yu Linjun , Luo Lianzhong , Zheng Yongbiao
Published: June 2023

In recent years, wild morel mushroom species have begun to be widely cultivated in China due to their high edible and medicinal values. To parse the medicinal ingredients, we employed the technique of liquid-submerged fermentation to investigate the secondary metabolites of Morehella importuna. Two new natural isobenzofuranone derivatives (1–2) and one new orsellinaldehyde derivative (3), together with seven known compounds, including one o-orsellinaldehyde (4), phenylacetic acid (5), benzoic acid (6), 4-hydroxy-phenylacetic acid (7), 3,5-dihydroxybenzoic acid (8), N,N′-pentane-1,5-diyldiacetamide (9), and 1H-pyrrole-2-carboxylic acid (10), were obtained from the fermented broth of M. importuna. Their structures were determined according to the data of NMR, HR Q-TOF MS, IR, UV, optical activity, and single-crystal X-ray crystallography. TLC-bioautography displayed that these compounds possess significant antioxidant activity with the half DPPH free radical scavenging concentration of 1.79 (1), 4.10 (2), 4.28 (4), 2.45 (5), 4.40 (7), 1.73 (8), and 6.00 (10) mM. The experimental results would shed light on the medicinal value of M. importuna for its abundant antioxidants.
Dual Anta-Inhibitors Targeting Protein Kinase CK1δ and A2A Adenosine Receptor Useful in Neurodegenerative Disorders
Authors: Francucci Beatrice , Angeloni Simone , Dal Ben Diego , Lambertucci Catia , Ricciutelli Massimo , Spinaci Andrea , Smirnov Aleksei , Volpini Rosaria , Buccioni Michela , Marucci Gabriella
Published: June 2023

Currently, the number of patients with neurodegenerative pathologies is estimated at over one million, with consequences also on the economic level. Several factors contribute to their development, including overexpression of A2A adenosine receptors (A2AAR) in microglial cells and up-regulation and post-translational alterations of some casein kinases (CK), among them, CK-1δ. The aim of the work was to study the activity of A2AAR and CK1δ in neurodegeneration using in-house synthesized A2A/CK1δ dual anta-inhibitors and to evaluate their intestinal absorption. Experiments were performed on N13 microglial cells, which were treated with a proinflammatory CK cocktail to simulate an inflammatory state typical of neurodegenerative diseases. Results showed that the dual anta-inhibitors have the ability to counteract the inflammatory state, even if compound 2 is more active than compound 1. In addition, compound 2 displayed an important antioxidant effect similar to the reference compound ZM241385. Since many known kinase inhibitors are very often unable to cross lipid bilayer membranes, the ability of A2A/CK1δ double anta-inhibitors to cross the intestinal barrier was investigated by an everted gut sac assay. HPLC analysis revealed that both compounds are able to cross the intestinal barrier, making them promising candidates for oral therapy.
Hydrothermal Carbon Coating of an Activated Carbon—A New Adsorbent
Authors: Adame-Pereira Marta , Durán-Valle Carlos J. , Fernández-González Carmen
Published: June 2023

A new adsorbent material was prepared by coating an activated carbon with hydrothermal carbon obtained from sucrose. The material obtained has different properties from the sum of the properties of the activated carbon and the hydrothermal carbon, which shows that a new material was obtained. It has a high specific surface area (1051.9 m2 g−1) and is slightly more acidic than the starting activated carbon (p.z.c.-point of zero charge 8.71 vs. 9.09). The adsorptive properties of a commercial carbon (Norit RX-3 Extra) were improved over a wide pH and temperature range. The capacity values of the monolayer according to Langmuir’s model reached 588 mg g−1 for the commercial product and 769 mg g−1 for the new adsorbent.
Bioactive Nanostructured Scaffold-Based Approach for Tendon and Ligament Tissue Engineering
Authors: Govindaraju Darshan Tagadur , Chen Chih-Hao , Shalumon K. T. , Kao Hao-Hsi , Chen Jyh-Ping
Published: June 2023

An effective therapeutic strategy to treat tendon or ligament injury continues to be a clinical challenge due to the limited natural healing capacity of these tissues. Furthermore, the repaired tendons or ligaments usually possess inferior mechanical properties and impaired functions. Tissue engineering can restore the physiological functions of tissues using biomaterials, cells, and suitable biochemical signals. It has produced encouraging clinical outcomes, forming tendon or ligament-like tissues with similar compositional, structural, and functional attributes to the native tissues. This paper starts by reviewing tendon/ligament structure and healing mechanisms, followed by describing the bioactive nanostructured scaffolds used in tendon and ligament tissue engineering, with emphasis on electrospun fibrous scaffolds. The natural and synthetic polymers for scaffold preparation, as well as the biological and physical cues offered by incorporating growth factors in the scaffolds or by dynamic cyclic stretching of the scaffolds, are also covered. It is expected to present a comprehensive clinical, biological, and biomaterial insight into advanced tissue engineering-based therapeutics for tendon and ligament repair.
Experimental, Theoretical and Numerical Studies on Thermal Properties of Lightweight 3D Printed Graphene-Based Discs with Designed Ad Hoc Air Cavities
Authors: Spinelli Giovanni , Guarini Rosella , Kotsilkova Rumiana , Ivanov Evgeni , Romano Vittorio
Published: June 2023

The current state of the art on material science emphasizes recent research efforts aimed at designing novel materials characterized by low-density and advanced properties. The present article reports the experimental, theoretical and simulation results on the thermal behavior of 3D printed discs. Filaments of pure poly (lactic acid) PLA and filled with 6 wt% of graphene nanoplatelets (GNPs) are used as feedstocks. Experiments indicate that the introduction of graphene enhances the thermal properties of the resulting materials since the conductivity passes from the value of 0.167 [W/mK] for unfilled PLA to 0.335 [W/mK] for reinforced PLA, which corresponds to a significantly improvement of 101%. Exploiting the potential of 3D printing, different air cavities have been intentionally designed to develop new lightweight and more cost-effective materials without compromising their thermal performances. Furthermore, some cavities are equal in volume but different in the geometry; it is necessary to investigate how this last characteristic and its possible orientations affect the overall thermal behavior compared to that of an air-free specimen. The influence of air volume is also investigated. Experimental results are supported by theoretical analysis and simulation studies based on the finite element method. The results aim to be a valuable reference resource in the field of design and optimization of lightweight advanced materials.
Contributions of a Rehabilitation Nursing Program in the Self-Care of Women Undergoing Breast Surgery
Authors: Rodrigues Tânia , Moreira Maria Teresa , Lima Andreia , Fernandes Rita , Gomes Bárbara
Published: June 2023

Background: Although surgical treatments for breast cancer have exhibited advanced interventions, axillary lymph node dissection can limit functionality and compromise women’s self-care. This study aims to assess the effectiveness of a rehabilitation nursing program in improving self-care performance in women undergoing breast surgery with axillary lymph node dissection. Methods: This quantitative, quasi-experimental study involved 48 women recruited from a main hospital between 2018 and 2019. The participants completed a three-month rehabilitation program at home. The evaluation instrument used was the DASH questionnaire. This study was not registered. Results: The functionality of the upper limb ipsilateral to the surgery improved significantly (p < 0.001) after the implementation of the program, influencing the participants’ capacity for self-care, including washing/drying their hair, washing their back, and wearing a shirt. The average DASH total score increased from 54.4 to 8.1 after the program. Conclusions: The rehabilitation nursing program positively influenced the participants’ self-care ability. Incorporating rehabilitation nursing programs into breast cancer treatment can improve self-care performance and the overall quality of patients’ lives. This study was not registered.
Actinomycetes as Producers of Biologically Active Terpenoids: Current Trends and Patents
Authors: Tarasova Ekaterina V. , Luchnikova Natalia A. , Grishko Victoria V. , Ivshina Irina B.
Published: June 2023

Terpenes and their derivatives (terpenoids and meroterpenoids, in particular) constitute the largest class of natural compounds, which have valuable biological activities and are promising therapeutic agents. The present review assesses the biosynthetic capabilities of actinomycetes to produce various terpene derivatives; reports the main methodological approaches to searching for new terpenes and their derivatives; identifies the most active terpene producers among actinomycetes; and describes the chemical diversity and biological properties of the obtained compounds. Among terpene derivatives isolated from actinomycetes, compounds with pronounced antifungal, antiviral, antitumor, anti-inflammatory, and other effects were determined. Actinomycete-produced terpenoids and meroterpenoids with high antimicrobial activity are of interest as a source of novel antibiotics effective against drug-resistant pathogenic bacteria. Most of the discovered terpene derivatives are produced by the genus Streptomyces; however, recent publications have reported terpene biosynthesis by members of the genera Actinomadura, Allokutzneria, Amycolatopsis, Kitasatosporia, Micromonospora, Nocardiopsis, Salinispora, Verrucosispora, etc. It should be noted that the use of genetically modified actinomycetes is an effective tool for studying and regulating terpenes, as well as increasing productivity of terpene biosynthesis in comparison with native producers. The review includes research articles on terpene biosynthesis by Actinomycetes between 2000 and 2022, and a patent analysis in this area shows current trends and actual research directions in this field.
Axial Resolution Enhancement of Optical Sectioning Structured Illumination Microscopy Based on Three-Beam Interference
Authors: Xiao Chao , Li Xing , Qian Jia , Ma Wang , Min Junwei , Gao Peng , Dan Dan , Yao Baoli
Published: June 2023

As a branch of 3D microscopy, optical sectioning structured illumination microscopy (OS-SIM) has the advantages of fast imaging speed, weak photobleaching and phototoxicity, and flexible and compatible configuration. Although the method of using the one-dimensional periodic fringe pattern projected on the sample can remove the out-of-focus background from the in-focus signal, the axial resolution of the final reconstructed 3D image is not improved. Here, we propose a three-beam interference OS-SIM, namely TBOS, instead of the common-used dual-beam interference OS-SIM (DBOS). The three-beam interference scheme has been adopted in 3D super-resolution SIM (3D-SR-SIM), where the fringe phase shifting needs to be along each of the three orientations. In contrast, TBOS applies phase shifting only in one arbitrary direction. We built a TBOS SIM microscope and performed the 3D imaging experiments with 46 nm diameter fluorescent microspheres and a mouse kidney section. The axial resolution of the 3D image obtained with TBOS was enhanced by a factor of 1.36 compared to the DBOS method, consistent with the theoretical analysis and simulation. The OS-SIM with enhanced axial resolution for 3D imaging may find a wide range of applications in the biomedical field.
Cultivation of Microalgae Chlorella vulgaris in Open Reactor for Bioethanol Production
Authors: Silva Graziella , Cerqueira Keilla , Rodrigues Jacqueline , Silva Karollyna , Coelho Diego , Souza Roberto
Published: June 2023

Microalgae have a high growth rate, high CO2 absorption capacity, and high content of chlorophyll, proteins, vitamins, mineral salts, carbohydrates, antioxidant substances, and fatty acids. In recent years, Chlorella vulgaris has been widely used as a feedstock for producing third-generation biofuels, such as bioethanol. Thus, this work aims to develop a strategy to increase the production scale of the microalgae Chlorella vulgaris grown in distilled reused water, supplemented with a modified BG-11 medium, to use biomass in the production of bioethanol. The total cultivation of 72 L presented a concentration of 0.415 g·L−1·d−1, with 61.32 g of final biomass. To improve carbohydrate extraction, the biomass was pre-treated with sulfuric acid at different concentrations (1.5% and 3% v/v). The hydrolyzed solution was supplemented with YPD (yeast extract peptone dextrose) medium and inoculated with Saccharomyces cerevisae yeast, initiating fermentation. In each sample, the Brix degree, cell concentration, reducing sugar concentration, and alcohol content were analyzed. The sample pre-treated with sulfuric acid 1.5% v/v was the one that presented the best result, with alcohol content after distillation of 68 °GL (Gay-Lussac). It appears that the cultivation of the microalgae Chlorella vulgaris in scale-up, with reused water, has high potential in the production of third-generation biofuel.
An Investigation into the Acute and Subacute Toxicity of Extracts of Cassipourea flanaganii Stem Bark In Vivo
Authors: Mpofana Nomakhosi , Chipangura John Kudakwashe , Paulse Michael , Yalo Masande , Gqaleni Nceba , Nxumalo Celenkosini Thembelenkosini , Dlova Ncoza Cordelia , Hussein Ahmed A. , Crouch Neil R.
Published: June 2023

The conventional use of medicinal plants is in part based on the widespread belief that plant crude extracts are non-toxic. In South Africa, traditional preparations of Cassipourea flanaganii used to treat hypermelanosis have accordingly been regarded by many as non-toxic. Whether that is so impacts on the potential of bark extracts to be developed as a commercial drug to treathypermelanosis, given their documented capacity to inhibit tyrosinase activity. Our study investigated the acute and subacute toxicity of the methanol extract of C. flanaganii bark in rats. Wistar rats were randomly assigned into different treatment groups. The rats received a daily oral gavage of crude extract for acute and subacute toxicity tests. Haematological, biomechanical, clinical and histopathology examinations were carried out to evaluate the possible toxicity of C. flanaganii. The results were subjected to the Student’s t-test and ANOVA. For both acute and subacute toxicity, there was no statistical difference between the groups. There were no clinical or behavioral signs of toxicity observed in the rats. No treatment-related gross pathology lesions and no histopathology were observed. The findings of this study demonstrate the absence of acute or subacute toxicity after oral treatment with C. flanaganii stem bark extracts in Wistar rats at the levels administered. Chemical profiling of the total extract using LC-MS tentatively identified eleven (11) compounds as the major chemical constituents.
Magnolin Inhibits Paclitaxel-Induced Cold Allodynia and ERK1/2 Activation in Mice
Authors: Kim Nari , Chung Geehoon , Son So-Ri , Park Jae Hyun , Lee Young Hyun , Park Keon-Tae , Cho Ik-Hyun , Jang Dae Sik , Kim Sun Kwang
Published: June 2023

Chemotherapy-induced peripheral neuropathy (CIPN) is a common side effect of anti-cancer drugs. The main symptoms often include sensory disturbances and neuropathic pain, and currently there is no effective treatment for this condition. This study aimed to investigate the suppressive effects of magnolin, an extracellular signal-regulated kinase (ERK) inhibitor substance derived from a 95% EtOH extract of the seeds of Magnolia denudata, on the symptoms of CIPN. A taxol-based anti-cancer drug paclitaxel (PTX) was repeatedly injected (2 mg/kg/day, total 8 mg/kg) into mice to induce CIPN. A neuropathic pain symptom was assessed using a cold allodynia test that scores behaviors of licking and shaking paw after plantar administration of acetone drop. Magnolin was administered intraperitoneally (0.1, 1, or 10 mg/kg) and behavioral changes to acetone drop were measured. The effect of magnolin administration on ERK expression in the dorsal root ganglion (DRG) was investigated using western blot analysis. The results showed that the repeated injections of PTX induced cold allodynia in mice. Magnolin administration exerted an analgesic effect on the PTX-induced cold allodynia and inhibited the ERK phosphorylation in the DRG. These results suggest that magnolin could be developed as an alternative treatment to suppress paclitaxel-induced neuropathic pain symptoms.
Genomic Analysis Highlights Putative Defective Susceptibility Genes in Tomato Germplasm
Authors: Li Ruiling , Maioli Alex , Lanteri Sergio , Moglia Andrea , Bai Yuling , Acquadro Alberto
Published: June 2023

Tomato (Solanum lycopersicum L.) is one of the most widely grown vegetables in the world and is impacted by many diseases which cause yield reduction or even crop failure. Breeding for disease resistance is thus a key objective in tomato improvement. Since disease arises from a compatible interaction between a plant and a pathogen, a mutation which alters a plant susceptibility (S) gene facilitating compatibility may induce broad-spectrum and durable plant resistance. Here, we report on a genome-wide analysis of a set of 360 tomato genotypes, with the goal of identifying defective S-gene alleles as a potential source for the breeding of resistance. A set of 125 gene homologs of 10 S-genes (PMR 4, PMR5, PMR6, MLO, BIK1, DMR1, DMR6, DND1, CPR5, and SR1) were analyzed. Their genomic sequences were examined and SNPs/indels were annotated using the SNPeff pipeline. A total of 54,000 SNPs/indels were identified, among which 1300 were estimated to have a moderate impact (non-synonymous variants), while 120 were estimated to have a high impact (e.g., missense/nonsense/frameshift variants). The latter were then analyzed for their effect on gene functionality. A total of 103 genotypes showed one high-impact mutation in at least one of the scouted genes, while in 10 genotypes, more than 4 high-impact mutations in as many genes were detected. A set of 10 SNPs were validated through Sanger sequencing. Three genotypes carrying high-impact homozygous SNPs in S-genes were infected with Oidium neolycopersici, and two highlighted a significantly reduced susceptibility to the fungus. The existing mutations fall within the scope of a history of safe use and can be useful to guide risk assessment in evaluating the effect of new genomic techniques.
Effects of Drip Irrigation-Fertilization on Growth, Flowering, Photosynthesis and Nutrient Absorption of Containerized Seedlings of Hibiscus syriacus L. (Haeoreum)
Authors: Jin Eon-Ju , Yoon Jun-Hyuck , Lee Hyeok , Kwon Hae-Yun , Shin Han-Na , Yong Seong-Hyeon , Choi Myung-Suk
Published: June 2023

The amount of irrigation and fertilization should be considered first for the production and standardization of high-quality H. syriacus L. seedlings using container seedlings. This study was conducted to investigate the optimal conditions suitable for container cultivation of hibiscus by analyzing growth and physiological responses according to the control of irrigation and fertilization. Therefore, in this study, H. syriacus L. for. Haeoreum (3-year-old hardwood cutting propagation), a fast-growing, was transplanted into a 40 L container. The irrigation amount per container was adjusted (0.2, 0.3 and 0.4 ton/yr/tree), and the amount of fertilizer applied (0, 69.0, 138.0 and 207.0 g/yr/tree). The growth rate according to the irrigation-fertilization treatment was higher in the 0.3 ton-138.0 g/yr/tree irrigation-fertilization treatment (p < 0.001). Total biomass yield and seedling quality index (SQI) were highest in the 0.3 ton-138.0 g/yr/tree irrigation-fertilization treatment (p < 0.001). The higher the fertilization concentration, the faster the flowering and the longer the flowering. The photosynthetic capacity of H. syriacus L. was reduced in bare root seedling cultivation and container-non-fertilized treatment. The chlorophyll fluorescence response was also affected by bare root cultivation and containerized seedling cultivation fertilization. Nutrient vector diagnosis showed “nutritional suitability” in the 0.3 ton-138.0 g/yr/tree treatment. Overall, containerized seedling cultivation was superior in growth, photosynthetic performance, photochemical efficiency, and nutrient storage capacity compared to bare root cultivation. These results be expected to contribute not only to the industrial production of excellent container seedlings of H. syriacus L. but also to the production of other woody plants.
Development and Characterization of a Novel Wheat–Tetraploid Thinopyrum elongatum 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage
Authors: Gong Biran , Zhao Lei , Zeng Chunyan , Zhu Wei , Xu Lili , Wu Dandan , Cheng Yiran , Wang Yi , Zeng Jian , Fan Xing , Sha Lina , Zhang Haiqin , Chen Guoyue , Zhou Yonghong , Kang Houyang
Published: June 2023

Stripe rust, which is caused by Puccinia striiformis f. sp. tritici, is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid Thinopyrum elongatum (2n = 4x = 28, EEEE) carries a variety of genes conferring resistance to multiple diseases, including stripe rust, Fusarium head blight, and powdery mildew, which makes it a valuable tertiary genetic resource for enhancing wheat cultivar improvement. Here, a novel wheat–tetraploid Th. elongatum 6E (6D) disomic substitution line (K17-1065-4) was characterized using genomic in situ hybridization and fluorescence in situ hybridization chromosome painting analyses. The evaluation of disease responses revealed that K17-1065-4 is highly resistant to stripe rust at the adult stage. By analyzing the whole-genome sequence of diploid Th. elongatum, we detected 3382 specific SSR sequences on chromosome 6E. Sixty SSR markers were developed, and thirty-three of them can accurately trace chromosome 6E of tetraploid Th. elongatum, which were linked to the disease resistance gene(s) in the wheat genetic background. The molecular marker analysis indicated that 10 markers may be used to distinguish Th. elongatum from other wheat-related species. Thus, K17-1065-4 carrying the stripe rust resistance gene(s) is a novel germplasm useful for breeding disease-resistant wheat cultivars. The molecular markers developed in this study may facilitate the mapping of the stripe rust resistance gene on chromosome 6E of tetraploid Th. elongatum.
Effectiveness of R1-nj Anthocyanin Marker in the Identification of In Vivo Induced Maize Haploid Embryos
Authors: Lopez Luis Antonio , Ochieng John , Pacheco Mario , Martinez Leocadio , Omar Hamilton Amoshe , Gowda Manje , Prasanna Boddupalli M. , Dhugga Kanwarpal S. , Chaikam Vijay
Published: June 2023

Doubled haploid (DH) technology has become integral to maize breeding programs to expedite inbred line development and increase the efficiency of breeding operations. Unlike many other plant species that use in vitro methods, DH production in maize uses a relatively simple and efficient in vivo haploid induction method. However, it takes two complete crop cycles for DH line generation, one for haploid induction and the other one for chromosome doubling and seed production. Rescuing in vivo induced haploid embryos has the potential to reduce the time for DH line development and improve the efficiency of DH line production. However, the identification of a few haploid embryos (~10%) resulting from an induction cross from the rest of the diploid embryos is a challenge. In this study, we demonstrated that an anthocyanin marker, namely R1-nj, which is integrated into most haploid inducers, can aid in distinguishing haploid and diploid embryos. Further, we tested conditions that enhance R1-nj anthocyanin marker expression in embryos and found that light and sucrose enhance anthocyanin expression, while phosphorous deprivation in the media had no affect. Validating the use of the R1-nj marker for haploid and diploid embryo identification using a gold standard classification based on visual differences among haploids and diploids for characteristics such as seedling vigor, erectness of leaves, tassel fertility, etc., indicated that the R1-nj marker could lead to significantly high false positives, necessitating the use of additional markers for increased accuracy and reliability of haploid embryo identification.
LeGRXS14 Reduces Salt Stress Tolerance in Arabidopsis thaliana
Authors: Liu Lulu , Li Xiaofei , Su Mengke , Shi Jiaping , Zhang Qing , Liu Xunyan
Published: June 2023

Salt stress represents a significant abiotic stressor for plants and poses a severe threat to agricultural productivity. Glutaredoxins (GRXs) are small disulfide reductases that can scavenge cellular reactive oxygen species and are crucial for plant growth and development, particularly under stressful circumstances. Although CGFS-type GRXs were found to be involved in various abiotic stresses, the intrinsic mechanism mediated by LeGRXS14, a tomato (Lycopersicon esculentum Mill.) CGFS-type GRX, is not yet fully understood. We discovered that LeGRXS14 is relatively conserved at the N-terminus and exhibits an increase in expression level under salt and osmotic stress conditions in tomatoes. The expression levels of LeGRXS14 in response to osmotic stress peaked relatively rapidly at 30 min, while the response to salt stress only peaked at 6 h. We constructed LeGRXS14 overexpression Arabidopsis thaliana (OE) lines and confirmed that LeGRXS14 is located on the plasma membrane, nucleus, and chloroplasts. In comparison to the wild-type Col-0 (WT), the OE lines displayed greater sensitivity to salt stress, resulting in a profound inhibition of root growth under the same conditions. Analysis of the mRNA levels of the WT and OE lines revealed that salt stress-related factors, such as ZAT12, SOS3, and NHX6, were downregulated. Based on our research, it can be concluded that LeGRXS14 plays a significant role in plant tolerance to salt. However, our findings also suggest that LeGRXS14 may act as a negative regulator in this process by exacerbating Na+ toxicity and the resulting oxidative stress.
Phytoextraction and Migration Patterns of Cadmium in Contaminated Soils by Pennisetum hybridum
Authors: Chen Canming , Wei Zebin , Hu Kuangzheng , Wu Qi-Tang
Published: June 2023

This study was conducted to identify soil cadmium (Cd) removal pathways and their contribution rates during phytoremediation by Pennisetum hybridum, as well as to comprehensively assess its phytoremediation potential. Multilayered soil column tests and farmland-simulating lysimeter tests were conducted to investigate the Cd phytoextraction and migration patterns in topsoil and subsoil simultaneously. The aboveground annual yield of P. hybridum grown in the lysimeter was 206 ton·ha−1. The total amount of Cd extracted in P. hybridum shoots was 234 g·ha−1, which was similar to that of other typical Cd-hyperaccumulating plants such as Sedum alfredii. After the test, the topsoil Cd removal rate was 21.50–35.81%, whereas the extraction efficiency in P. hybridum shoots was only 4.17–8.53%. These findings indicate that extraction by plant shoots is not the most important contributor to the decrease of Cd in the topsoil. The proportion of Cd retained by the root cell wall was approximately 50% of the total Cd in the root. Based on column test results, P. hybridum treatment led to a significant decrease in soil pH and considerably enhanced Cd migration to subsoil and groundwater. P. hybridum decreases Cd in the topsoil through multiple pathways and provides a relatively ideal material for phytoremediation of Cd-contaminated acid soils.
Photosynthetic Acclimation of Shade-Grown Soybean Seedlings to a High-Light Environment
Authors: Su Yahan , Yang Huan , Wu Yushan , Gong Wanzhuo , Gul Hina , Yan Yanhong , Yang Wenyu
Published: June 2023

Soybean in relay intercropping is initially exposed to a shade environment, followed by exposure to full sunlight after the harvesting of primary crops, e.g., maize. Therefore, soybean’s ability to acclimate to this changing light environment determines its growth and yield formation. However, the changes in soybean photosynthesis under such light alternations in relay intercropping are poorly understood. This study compared the photosynthetic acclimation of two soybean varieties with contrasting shade tolerance, i.e., Gongxuan1 (shade-tolerant) and C103 (shade-intolerant). The two soybean genotypes were grown in a greenhouse under full sunlight (HL) and 40% full sunlight (LL) conditions. Subsequently, after the fifth compound leaf expanded, half of the LL plants were transferred to a high-sunlight environment (LL-HL). Morphological traits were measured at 0 and 10 days, while chlorophyll content, gas exchange characteristics and chlorophyll fluorescence were assayed at 0, 2, 4, 7 and 10 days after transfer to an HL environment (LL-HL). Shade-intolerant C103 showed photoinhibition 10 days after transfer, and the net photosynthetic rate (Pn) did not completely recover to that under a high light level. On the day of transfer, the shade-intolerant variety, C103, exhibited a decrease in net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (E) in the low-light (LL) and low-light-to-high-light (LL-HL) treatments. Additionally, intercellular CO2 concentration (Ci) increased in low light, suggesting that non-stomatal factors were the primary limitations to photosynthesis in C103 following the transfer. In contrast, the shade-tolerant variety, Gongxuan1, displayed a greater increase in Pn 7 days after transfer, with no difference observed between the HL and LL-HL treatments. Ten days after transfer, the shade-tolerant Gongxuan1 exhibited 24.1%, 10.9% and 20.9% higher biomass, leaf area and stem diameter than the intolerant C103. These findings suggest that Gongxuan1 possesses a higher capacity to adapt to variations in light conditions, making it a potential candidate for variety selection in intercropping systems.
The Splicing Factor OsSCL26 Regulates Phosphorus Homeostasis in Rice
Authors: Lu Shanshan , Ye Jun , Li Hui , He Fengyu , Qi Yue , Wang Ting , Wang Wujian , Zheng Luqing
Published: June 2023

Phosphorus (P) is an essential nutrient for plant growth. However, its deficiency poses a significant challenge for crop production. To overcome the low P availability, plants have developed various strategies to regulate their P uptake and usage. In this study, we identified a splicing factor, OsSCL26, belonging to the Serine/arginine-rich (SR) proteins, that plays a crucial role in regulating P homeostasis in rice. OsSCL26 is expressed in the roots, leaves, and base nodes, with higher expression levels observed in the leaf blades during the vegetative growth stage. The OsSCL26 protein is localized in the nucleus. Mutation of OsSCL26 resulted in the accumulation of P in the shoot compared to the wild-type, and the dwarf phenotype of the osscl26 mutant was alleviated under low P conditions. Further analysis revealed that the accumulated P concentrations in the osscl26 mutant were higher in the old leaves and lower in the new leaves. Furthermore, the P-related genes, including the PHT and SPX family genes, were upregulated in the osscl26 mutant, and the exclusion/inclusion ratio of the two genes, OsSPX-MFS2 and OsNLA2, was increased compared to wild-type rice. These findings suggest that the splicing factor OsSCL26 plays a pivotal role in maintaining P homeostasis in rice by influencing the absorption and distribution of P through the regulation of the transcription and splicing of the P transport genes.
Extracts from the Leaf of Couroupita guianensis (Aubl.): Phytochemical, Toxicological Analysis and Evaluation of Antioxidant and Antimicrobial Activities against Oral Microorganisms
Authors: Augusco Marco Aurélio Carmona , Sarri Daniela Abram , Panontin Juliane Farinelli , Rodrigues Maria Angélica Melo , Fernandes Rachel de Moura Nunes , Silva Juliana Fonseca Moreira da , Cardoso Claudia Andrea Lima , Rambo Magale Karine Diel , Scapin Elisandra
Published: June 2023

The study of phytotherapy in dentistry holds great relevance because of the scarcity of research conducted on the treatment of oral pathologies, specifically, caries and periodontal disease. Therefore, this research aimed to analyze the chemical composition of extracts from Couroupita guianensis Aubl. leaves, evaluate their toxicity, and assess their antioxidant and antimicrobial properties against Staphylococcus aureus, Streptococcus mutans, and Candida albicans. Three extracts were prepared using assisted ultrasound and the Soxhlet apparatus, namely, Crude Ultrasound Extract (CUE), Crude Soxhlet Extract (CSE), and the Ethanol Soxhlet Extract (ESE). Flavonoids, tannins, and saponins were detected in the chemical analysis, while LC-DAD analysis revealed the presence of caffeic acid, sinapic acid, rutin, quercetin, luteolin, kaempferol, and apigenin in all extracts. GC-MS analysis identified stigmasterol and β-sitosterol in the CUE and CSE. The ESE showed higher antioxidant activity (2.98 ± 0.96 and 4.93 ± 0.90) determined by the DPPH• and ABTS•+ methods, respectively. In the toxicity evaluation, the CUE at 50 μg/mL and the ESE at 50 μg/mL stimulated the growth of Allium cepa roots, while all extracts inhibited root growth at 750 μg/mL. None of the extracts exhibited toxicity against Artemia salina. Antibacterial activity was observed in all extracts, particularly against the microorganisms S. aureus and S. mutans. However, no antifungal activity against C. albicans was detected. These results suggest that extracts of C. guianensis have therapeutic potential for controlling microorganisms in the oral microbiota.
Hydrothermal Synthesis of Functionalized Carbon Nanodots and Their Clusters as Ionic Probe for High Sensitivity and Selectivity for Sulfate Anions with Excellent Detection Level
Authors: Yang Po-Chih , Panda Pradeep Kumar , Li Cheng-Han , Ting Yu-Xuan , Ashraf Gandomi Yasser , Hsieh Chien-Te
Published: June 2023

Nitrogen-doped carbon nanodots (CNDs) were synthesized and utilized as sensing probes to detect different anions and metallic ions within aqueous solutions. The pristine CNDs were developed through a one-pot hydrothermal synthesis. o-Phenylenediamine was used as the precursor. A similar hydrothermal synthesis technique in the presence of polyethylene glycol (PEG) was adopted to form the PEG-coated CND clusters (CND-100k). Through photoluminescence (PL) quenching, both CND and PEG-coated CND suspensions display ultra-high sensitivity and selectivity towards HSO4− anions (Stern–Volmer quenching constant (KSV) value: 0.021 ppm−1 for CND and 0.062 ppm−1 for CND-100k) with an ultra-low detection limit (LOD value: 0.57 ppm for the CND and 0.19 ppm for CND-100k) in the liquid phase. The quenching mechanism of N-doped CNDs towards HSO4− ions involves forming the bidentate as well as the monodentate hydrogen bonding with the sulfate anionic moieties. The detection mechanism of metallic ions analyzed through the Stern–Volmer formulation reveals that the CND suspension is well suited for the detection of Fe3+ (KSV value: 0.043 ppm−1) and Fe2+ (KSV value: 0.0191 ppm−1) ions, whereas Hg2+ (KSV value: 0.078 ppm−1) sensing can be precisely performed by the PEG-coated CND clusters. Accordingly, the CND suspensions developed in this work can be employed as high-performance PL probes for detecting various anions and metallic ions in the liquid phase.
3D Printed Supercapacitor Exploiting PEDOT-Based Resin and Polymer Gel Electrolyte
Authors: Bertana Valentina , Scordo Giorgio , Camilli Elena , Ge Limeng , Zaccagnini Pietro , Lamberti Andrea , Marasso Simone Luigi , Scaltrito Luciano
Published: June 2023

Renewable energy-based technologies and increasing IoT (Internet of Things) objects population necessarily require proper energy storage devices to exist. In the view of customized and portable devices, Additive Manufacturing (AM) techniques offer the possibility to fabricate 2D to 3D features for functional applications. Among the different AM techniques extensively explored to produce energy storage devices, direct ink writing is one of the most investigated, despite the poor achievable resolution. Herein, we present the development and characterization of an innovative resin which can be employed in a micrometric precision stereolithography (SL) 3D printing process for the fabrication of a supercapacitor (SC). Poly(3,4-ethylenedioxythiophene) (PEDOT), a conductive polymer, was mixed with poly(ethylene glycol) diacrylate (PEGDA), to get a printable and UV curable conductive composite material. The 3D printed electrodes were electrically and electrochemically investigated in an interdigitated device architecture. The electrical conductivity of the resin falls within the range of conductive polymers with 200 mS/cm and the 0.68 µWh/cm2 printed device energy density falls within the literature range.
Wall Materials for Encapsulating Bioactive Compounds via Spray-Drying: A Review
Authors: Díaz-Montes Elsa
Published: June 2023

Spray-drying is a continuous encapsulation method that effectively preserves, stabilizes, and retards the degradation of bioactive compounds by encapsulating them within a wall material. The resulting capsules exhibit diverse characteristics influenced by factors such as operating conditions (e.g., air temperature and feed rate) and the interactions between the bioactive compounds and the wall material. This review aims to compile recent research (within the past 5 years) on spray-drying for bioactive compound encapsulation, emphasizing the significance of wall materials in spray-drying and their impact on encapsulation yield, efficiency, and capsule morphology.