bluuebunny/crossref_metadata_2025 huggingface dataset:
Phospholipase A2 from bee venom increases poly(I:C)-induced activation in human keratinocytes
Authors: Nakashima Akina , Tomono Susumu , Yamazaki Tatsuya , Inui Masanori , Morita Naoko , Ichimonji Isao , Takagi Hidekazu , Nagaoka Fumiaki , Matsumoto Misako , Ito Yasuhiko , Yanagishita Takeshi , Miyake Kensuke , Watanabe Daisuke , Akashi-Takamura Sachiko
Published: January 2020

Bee venom (BV) induces skin inflammation, characterized by erythema, blisters, edemas, pain and itching. Although BV has been found to have an inhibitory effect on toll-like receptors (TLRs), we here show that BV enhances keratinocyte responses to polyinosinic-polycytidylic acid [poly(I:C)], a ligand for TLR3. Our results revealed that the enhanced TLR activity was primarily induced by secretory phospholipase A2 (sPLA2), a component of BV (BV-sPLA2). PLA2 mediates the hydrolysis of membrane phospholipids into lysophospholipids and free fatty acids. We demonstrated that BV-sPLA2 increased the intracellular uptake of poly(I:C), phosphorylation of the nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs), and poly(I:C)-mediated interleukin 8 production in human keratinocytes. We further showed that the enzymatic activity of BV-sPLA2 was essential for the increased uptake of poly(I:C). These findings suggest that BV-sPLA2 may induce a modification of the cell membrane structure, leading to enhanced poly(I:C) uptake in keratinocytes. BV-sPLA2 might be able to promote wound healing by enhancing TLR3 responses.
Overexpression of RSK4 reverses doxorubicin resistance in human breast cancer cells via PI3K/AKT signalling pathway
Authors: Mei Yan , Liao Xiaoming , Zhu Lingyu , Yang Huawei
Published: January 2020

Doxorubicin (DOX) is one of the most effective chemotherapy drugs for the treatment of metastatic breast cancer (BC), but drug resistance becomes an obstacle to treatment. This study aims to investigate the role of Ribosomal S6 protein kinase 4 (RSK4) in regulating BC resistance to DOX. We first used Kaplan–Meier Plotter to identify the prognostic roles of RSK4 in BC. DOX-resistant BC cells (MCF-7/DOX) were constructed and the expression of RSK4 was determined by reverse transcript polymerase chain reaction and western blot. Subsequently, we overexpressed the RSK4 in MCF-7/DOX cells, and measured drug resistance, colony formation, cell migration, invasion ability and cell apoptosis after transfection. In addition, western blot was used to explore the expression of apoptosis-related proteins and BC-resistance protein. Effects of RSK4 on activation of the PI3K/AKT signalling pathway were also tested. Furthermore, tumour xenograft in nude mice was constructed to observe the effect of RSK4 overexpression on tumour growth in vivo. In conclusion, RSK4 was positively correlated with survival rate in BC patients, which is lowly expressed in MCF-7/DOX. Meanwhile, the overexpression of RSK4 may inhibit drug resistance, cell migration, invasion, apoptosis and tumour growth. RSK4 may effectively attenuate DOX resistance in BC by inhibiting the PI3K/AKT signalling pathway.
For the Benefit of Students: Memory and Anatomical Learning at Bologna in the Fourteenth to Early Sixteenth Centuries
Authors: Robison Kira L
Published: January 2020

The anatomical textbook in the late Middle Ages was one part of a greater pedagogical process that involved students’ seeing, hearing, reading, and eventually knowing information about the human body. By examining the role of the anatomical textbook and accompanying bodily images in anatomical learning, this article illuminates the complexity and self-consciousness of anatomical education in the medieval university, as professors focused on ways to enhance student memory of the material. Traditionally, the history of anatomy has been heavily influenced by the anatomical Renaissance of the late-sixteenth century, highlighting a focus on innovative medical knowledge and the scientific method. However, if we engage a pedagogical lens when looking at these medieval authors, it becomes quickly obvious that the whole point of university medicine was not to explore unknown boundaries and discover new ideas of medicine, but rather to communicate the current and established body of knowledge to those not familiar with it.
Evolving pulsation of the slowly rotating magnetic β Cep star ξ1 CMa
Authors: Wade G A , Pigulski A , Begy S , Shultz M , Handler G , Sikora J , Neilson H , Cugier H , Erba C , Moffat A F J , Pablo B , Popowicz A , Weiss W , Zwintz K
Published: January 2020

Recent BRITE-Constellation space photometry of the slowly rotating, magnetic β Cep pulsator ξ1 CMa permits a new analysis of its pulsation properties. Analysis of the two-colour BRITE data reveals the well-known single pulsation period of 0.209 d, along with its first and second harmonics. A similar analysis of SMEI and TESS observations yields compatible results, with the higher precision TESS observations also revealing several low-amplitude modes with frequencies below 5 d−1; some of these are likely g modes. The phase lag between photometric and radial velocity maxima – equal to 0.334 cycles – is significantly larger than the typical value of 1/4 observed in other large-amplitude β Cep stars. The phase lag, as well as the strong dependence of phase of maximum light on wavelength, can be reconciled with seismic models only if the dominant mode is the fundamental radial mode. We employ all published photometric and radial velocity measurements, spanning over a century, to evaluate the stability of the pulsation period. The O−C diagram exhibits a clear parabolic shape consistent with a mean rate of period change $\dot{P}=0.34\pm 0.02$ s cen−1. The residuals from the best-fitting parabola exhibit scatter that is substantially larger than the uncertainties. In particular, dense sampling obtained during the past ∼20 yr suggests more complex and rapid period variations. Those data cannot be coherently phased with the mean rate of period change, and instead require $\dot{P}\sim 0.9$ s cen−1. We examine the potential contributions of binarity, stellar evolution, and stellar rotation and magnetism to understand the apparent period evolution.
Cradles of the first stars: self-shielding, halo masses, and multiplicity
Authors: Skinner Danielle , Wise John H
Published: January 2020

The formation of Population III (Pop III) stars is a critical step in the evolution of the early Universe. To understand how these stars affected their metal-enriched descendants, the details of how, why and where Pop III formation takes place needs to be determined. One of the processes that is assumed to greatly affect the formation of Pop III stars is the presence of a Lyman–Werner (LW) radiation background, that destroys H2, a necessary coolant in the creation of Pop III stars. Self-shielding can alleviate the effect the LW background has on the H2 within haloes. In this work, we perform a cosmological simulation to study the birthplaces of Pop III stars, using the adaptive mesh refinement code enzo. We investigate the distribution of host halo masses and its relationship to the LW background intensity. Compared to previous work, haloes form Pop III stars at much lower masses, up to a factor of a few, due to the inclusion of H2 self-shielding. We see no relationship between the LW intensity and host halo mass. Most haloes form multiple Pop III stars, with a median number of four, up to a maximum of 16, at the instance of Pop III formation. Our results suggest that Pop III star formation may be less affected by LW radiation feedback than previously thought and that Pop III multiple systems are common.
The Pristine Survey – VIII. The metallicity distribution function of the Milky Way halo down to the extremely metal-poor regime
Authors: Youakim K , Starkenburg E , Martin N F , Matijevič G , Aguado D S , Allende Prieto C , Arentsen A , Bonifacio P , Carlberg R G , González Hernández J I , Hill V , Kordopatis G , Lardo C , Navarro J F , Jablonka P , Sánchez Janssen R , Sestito F , Thomas G F , Venn K
Published: January 2020

The Pristine survey uses narrow-band photometry to derive precise metallicities down to the extremely metal-poor regime ($ \rm [Fe/H] \lt -3$), and currently consists of over 4 million FGK-type stars over a sky area of $\sim 2500\, \mathrm{deg}^2$. We focus our analysis on a subsample of ∼80 000 main-sequence turn-off stars with heliocentric distances between 6 and 20 kpc, which we take to be a representative sample of the inner halo. The resulting metallicity distribution function (MDF) has a peak at $ \rm [Fe/H] =-1.6$, and a slope of Δ(LogN)/$\Delta \rm [Fe/H] = 1.0 \pm 0.1$ in the metallicity range of $-3.4\; \lt\; \rm [Fe/H]\; \lt -2.5$. This agrees well with a simple closed-box chemical enrichment model in this range, but is shallower than previous spectroscopic MDFs presented in the literature, suggesting that there may be a larger proportion of metal-poor stars in the inner halo than previously reported. We identify the Monoceros/TriAnd/ACS/EBS/A13 structure in metallicity space in a low-latitude field in the anticentre direction, and also discuss the possibility that the inner halo is dominated by a single, large merger event, but cannot strongly support or refute this idea with the current data. Finally, based on the MDF of field stars, we estimate the number of expected metal-poor globular clusters in the Milky Way halo to be 5.4 for $ \rm [Fe/H]\; \lt\; -2.5$ and 1.5 for $ \rm [Fe/H]\; \lt\; -3$, suggesting that the lack of low-metallicity globular clusters in the Milky Way is not due simply to statistical undersampling.
Simulated mass measurements of the young planet K2-33b
Authors: Klein Baptiste , Donati J-F
Published: January 2020

In this paper, we carry out simulations of radial velocity (RV) measurements of the mass of the 8–11 Myr Neptune-sized planet K2-33b using high-precision near-infrared velocimeters like SPIRou at the Canada–France–Hawaii Telescope. We generate an RV curve containing a planet signature and a realistic stellar activity signal, computed for a central wavelength of 1.8 µm and statistically compatible with the light curve obtained with K2. The modelled activity signal includes the effect of time-evolving dark and bright surface features hosting a 2 kG radial magnetic field, resulting in an RV signal of semi-amplitude ∼30 m s−1. Assuming a 3-month visibility window, we build RV time series including Gaussian white noise from which we retrieve the planet mass while filtering the stellar activity signal using Gaussian process regression. We find that 35/50 visits spread over three consecutive bright-time runs on K2-33 allow one to reliably detect planet RV signatures of, respectively, 10 and 5 m s−1 at precisions >3σ. We also show that 30 visits may end up being insufficient in some cases to provide a good coverage of the stellar rotation cycle, with the result that the planet signature can go undetected or the mass estimation be plagued by large errors.
Evolution of Transcriptional Repressors Impacts Caenorhabditis Vulval Development
Authors: Chamberlin Helen M , Jain Ish M , Corchado-Sonera Marcos , Kelley Leanne H , Sharanya Devika , Jama Abdulrahman , Pabla Romy , Dawes Adriana T , Gupta Bhagwati P
Published: January 2020

Comparative genomic sequence analysis has found that the genes for many chromatin-associated proteins are poorly conserved, but the biological consequences of these sequence changes are not understood. Here, we show that four genes identified for an Inappropriate Vulval cell Proliferation (ivp) phenotype in the nematode Caenorhabditis briggsae exhibit distinct functions and genetic interactions when compared with their orthologs in C. elegans. Specifically, we show that the four C. briggsae ivp genes encode the noncanonical histone HTZ-1/H2A.z and three nematode-specific proteins predicted to function in the nucleus. The mutants exhibit ectopic vulval precursor cell proliferation (the multivulva [Muv] phenotype) due to inappropriate expression of the lin-3/EGF gene, and RNAseq analysis suggests a broad role for these ivp genes in transcriptional repression. Importantly, although the C. briggsae phenotypes have parallels with those seen in the C. elegans synMuv system, except for the highly conserved HTZ-1/H2A.z, comparable mutations in C. elegans ivp orthologs do not exhibit synMuv gene interactions or phenotypes. These results demonstrate the evolutionary changes that can underlie conserved biological outputs and argue that proteins critical to repress inappropriate expression from the genome participate in a rapidly evolving functional landscape.
Evolutionary Dynamics of Abundant 7-bp Satellites in the Genome ofDrosophila virilis
Authors: Flynn Jullien M , Long Manyuan , Wing Rod A , Clark Andrew G
Published: January 2020

The factors that drive the rapid changes in abundance of tandem arrays of highly repetitive sequences, known as satellite DNA, are not well understood. Drosophila virilis has one of the highest relative amounts of simple satellites of any organism that has been studied, with an estimated >40% of its genome composed of a few related 7-bp satellites. Here, we use D. virilis as a model to understand technical biases affecting satellite sequencing and the evolutionary processes that drive satellite composition. By analyzing sequencing data from Illumina, PacBio, and Nanopore platforms, we identify platform-specific biases and suggest best practices for accurate characterization of satellites by sequencing. We use comparative genomics and cytogenetics to demonstrate that the highly abundant AAACTAC satellite family arose from a related satellite in the branch leading to the virilis phylad 4.5–11 Ma before exploding in abundance in some species of the clade. The most abundant satellite is conserved in sequence and location in the pericentromeric region but has diverged widely in abundance among species, whereas the satellites nearest the centromere are rapidly turning over in sequence composition. By analyzing multiple strains of D. virilis, we saw that the abundances of two centromere-proximal satellites are anticorrelated along a geographical gradient, which we suggest could be caused by ongoing conflicts at the centromere. In conclusion, we illuminate several key attributes of satellite evolutionary dynamics that we hypothesize to be driven by processes including selection, meiotic drive, and constraints on satellite sequence and abundance.
ADAPT identifies an ESCRT complex composition that discriminates VCaP from LNCaP prostate cancer cell exosomes
Authors: Hornung Tassilo , O’Neill Heather A , Logie Stephen C , Fowler Kimberly M , Duncan Janet E , Rosenow Matthew , Bondre Aniket S , Tinder Teresa , Maher Varun , Zarkovic Jelena , Zhong Zenyu , Richards Melissa N , Wei Xixi , Miglarese Mark R , Mayer Günter , Famulok Michael , Spetzler David
Published: January 2020

Libraries of single-stranded oligodeoxynucleotides (ssODNs) can be enriched for sequences that specifically bind molecules on naïve complex biological samples like cells or tissues. Depending on the enrichment strategy, the ssODNs can identify molecules specifically associated with a defined biological condition, for example a pathological phenotype, and thus are potentially useful for biomarker discovery. We performed ADAPT, a variant of SELEX, on exosomes secreted by VCaP prostate cancer cells. A library of ∼1011 ssODNs was enriched for those that bind to VCaP exosomes and discriminate them from exosomes derived from LNCaP prostate cancer cells. Next-generation sequencing (NGS) identified the best discriminating ssODNs, nine of which were resynthesized and their discriminatory ability confirmed by qPCR. Affinity purification with one of the sequences (Sequence 7) combined with LC–MS/MS identified its molecular target complex, whereof most proteins are part of or associated with the multiprotein ESCRT complex participating in exosome biogenesis. Within this complex, YBX1 was identified as the directly-bound target protein. ADAPT thus is able to differentiate exosomes from cancer cell subtypes from the same lineage. The composition of ESCRT complexes in exosomes from VCaP versus LNCaP cells might constitute a discriminatory element between these prostate cancer subtypes.
Modulated control of DNA supercoiling balance by the DNA-wrapping domain of bacterial gyrase
Authors: Hobson Matthew J , Bryant Zev , Berger James M
Published: January 2020

Negative supercoiling by DNA gyrase is essential for maintaining chromosomal compaction, transcriptional programming, and genetic integrity in bacteria. Questions remain as to how gyrases from different species have evolved profound differences in their kinetics, efficiency, and extent of negative supercoiling. To explore this issue, we analyzed homology-directed mutations in the C-terminal, DNA-wrapping domain of the GyrA subunit of Escherichia coli gyrase (the ‘CTD’). The addition or removal of select, conserved basic residues markedly impacts both nucleotide-dependent DNA wrapping and supercoiling by the enzyme. Weakening CTD–DNA interactions slows supercoiling, impairs DNA-dependent ATP hydrolysis, and limits the extent of DNA supercoiling, while simultaneously enhancing decatenation and supercoil relaxation. Conversely, strengthening DNA wrapping does not result in a more extensively supercoiled DNA product, but partially uncouples ATP turnover from strand passage, manifesting in futile cycling. Our findings indicate that the catalytic cycle of E. coli gyrase operates at high thermodynamic efficiency, and that the stability of DNA wrapping by the CTD provides one limit to DNA supercoil introduction, beyond which strand passage competes with ATP-dependent supercoil relaxation. These results highlight a means by which gyrase can evolve distinct homeostatic supercoiling setpoints in a species-specific manner.
Use of radiomics for the prediction of local control of brain metastases after stereotactic radiosurgery
Authors: Mouraviev Andrei , Detsky Jay , Sahgal Arjun , Ruschin Mark , Lee Young K , Karam Irene , Heyn Chris , Stanisz Greg J , Martel Anne L
Published: January 2020

Background

Local response prediction for brain metastases (BM) after stereotactic radiosurgery (SRS) is challenging, particularly for smaller BM, as existing criteria are based solely on unidimensional measurements. This investigation sought to determine whether radiomic features provide additional value to routinely available clinical and dosimetric variables to predict local recurrence following SRS.

Methods

Analyzed were 408 BM in 87 patients treated with SRS. A total of 440 radiomic features were extracted from the tumor core and the peritumoral regions, using the baseline pretreatment volumetric post-contrast T1 (T1c) and volumetric T2 fluid-attenuated inversion recovery (FLAIR) MRI sequences. Local tumor progression was determined based on Response Assessment in Neuro-Oncology‒BM criteria, with a maximum axial diameter growth of >20% on the follow-up T1c indicating local failure. The top radiomic features were determined based on resampled random forest (RF) feature importance. An RF classifier was trained using each set of features and evaluated using the area under the receiver operating characteristic curve (AUC).

Results

The addition of any one of the top 10 radiomic features to the set of clinical features resulted in a statistically significant (P < 0.001) increase in the AUC. An optimized combination of radiomic and clinical features resulted in a 19% higher resampled AUC (mean = 0.793; 95% CI = 0.792–0.795) than clinical features alone (0.669, 0.668–0.671).

Conclusions

The increase in AUC of the RF classifier, after incorporating radiomic features, suggests that quantitative characterization of tumor appearance on pretreatment T1c and FLAIR adds value to known clinical and dosimetric variables for predicting local failure.
The Orestes Melt Zone, McMurdo Dry Valleys, Antarctica: Spatially Distributed Melting Regimes in a Contact Melt Zone, with Implications for the Formation of Rapakivi and Albite Granites
Authors: Currier R M , Flood T P
Published: January 2020

The Orestes Melt Zone (OMZ) is a massive contact melt zone (∼20 m thick by several kilometers long), located in the McMurdo Dry Valleys of Antarctica. The OMZ formed at shallow crustal depths by melting of the A-type Orestes Granite owing to intrusion of the underlying, doleritic Basement Sill. The OMZ can be divided broadly into two melting facies. The upper melting facies is distal from the contact and formed by melting at low temperature and water-saturated, or near water-saturated, conditions. The lower melting facies is proximal to the contact and formed by melting at high temperature and water-undersaturated conditions. Separate melting reactions occurred in both of the melting facies, resulting in distinct textures and melt compositions. Melting in the distal facies generated melts with compositions that plot near a predicted eutectic composition. Melting in the proximal facies was accomplished in part by replacement reactions in restitic feldspars. These reactions resulted in the development of plagioclase mantles on both restitic plagioclase and K-feldspar, and melt compositions that diverged from predicted minimum melt along an unexpected path, towards enrichment in orthoclase component. Thermal modeling indicates that this melt zone was active for a minimum of ∼150 years, with a contact temperature of ∼900 °C. Upon cooling, recrystallization generated ocellar textures around restitic quartz, as well as faceted albite as a late-stage product. Observations of the OMZ, combined with thermal modeling, provide new insights into the origin of rapakivi and albite granites. This study has implications for the origin of these two associated granite types in other geological settings.
Epidemiology, morbidity and mortality in Behçet’s disease: a cohort study using The Health Improvement Network (THIN)
Authors: Thomas Tom , Chandan Joht Singh , Subramanian Anuradhaa , Gokhale Krishna , Gkoutos George , Harper Lorraine , Buckley Christopher , Chandratre Priyanka , Raza Karim , Situnayake Deva , Nirantharakumar Krishnarajah
Published: January 2020

Objectives

The epidemiology of Behçet’s disease (BD) has not been well characterized in the UK. Evidence on the risk of cardiovascular disease, thromboembolic disease and mortality in patients with BD compared with the general population is scarce.

Methods

We used a large UK primary care database to investigate the epidemiology of BD. A retrospective matched cohort study was used to assess the following outcomes: risk of cardiovascular, thromboembolic disease and mortality. Controls were selected at a 1:4 ratio (age and gender matched). Cox proportional hazard models were used to derive adjusted hazard ratios (aHR).

Results

The prevalence of BD was 14.61 (95% CI 13.35–15.88) per 100 000 population in 2017. A total of 1281 patients with BD were compared with 5124 age- and gender-matched controls. There was significantly increased risk of ischaemic heart disease [aHR 3.09 (1.28–7.44)], venous thrombosis [aHR 4.80 (2.42–9.54)] and mortality [aHR 1.40 (1.07–1.84)] in patients with BD compared with corresponding controls. Patients with BD were at higher risk of pulmonary embolism compared with corresponding controls at baseline [adjusted odds ratio 4.64 (2.66–8.09), P < 0.0001]. The majority of patients with pulmonary embolism and a diagnosis of BD had pulmonary embolism preceding the diagnosis of BD, not after (87.5%; n = 28/32).

Conclusion

BD has a higher prevalence than previously thought. Physicians should be aware of the increased risk of developing ischaemic heart disease, stroke/transient ischaemic attack and deep venous thrombosis in patients with BD at an earlier age compared with the general population. Risk of embolism in patients with BD might vary across the disease course.
Frontal alpha asymmetry in alcohol-related intimate partner violence
Authors: Fink Brandi C , Howell Breannan C , Salway Sarah , Cavanagh James F , Hamilton Derek A , Claus Eric D , Frost Madeline E
Published: January 2020

Electroencephalographic (EEG) frontal alpha asymmetry (FAA) has been associated with differences in the experience and expression of emotion, motivation and anger in normal and clinical populations. The current study is the first to investigate FAA in alcohol-related intimate partner violence. EEG was recorded from 23 distressed violent (DV) and 15 distressed nonviolent (DNV) partners during a placebo-controlled alcohol administration and emotion-regulation study. The State-Trait Anger Expression Inventory 2 was used to evaluate anger experiences and was collected from both participants and their partners. During baseline, acute alcohol intoxication DV partners had significantly greater right FAA, whereas DNV partners showed greater left FAA. Both partner types demonstrated significantly greater right FAA during the placebo beverage condition of the emotion-regulation task when viewing evocative partner displays of contempt, belligerence, criticism, defensiveness and stonewalling, but greater left FAA during acute alcohol intoxication. Although no group differences were found in the emotion-regulation task, partner self-reported anger experiences accounted for 67% of the variance in the FAA of DV participants when intoxicated and viewing evocative stimuli, suggesting dyadic processes are important in understanding alcohol-related IPV. These findings suggest that FAA could index the affective and motivational determinants through which alcohol is related to IPV.
Chairs of academic events: The investments and academic impact
Authors: Trøst Hansen Thomas , Ren Carina
Published: January 2020

Every year, tens of thousands of academics engage in unfamiliar tasks related to catering, hotel booking, and transportation. They do so as chairs of the academic event. We do not know much about these chairmanships; neither how the researchers engage nor whether it is worthwhile from an academic point of view. Based on interviews with twenty-three researchers at six Danish universities and an analytical framework informed by the concept of credibility cycles, we analyze the academic chairmanship and how it impacts the knowledge production process of the chair. The article argues that the chairmanship is a multifaceted investment, which includes a range of non-academic tasks. The investment is a source for the following forms of credibility network, buzz, and recognition and the chairs gain access to a range of other potential exchanges. The study concludes that chairmanships of academic events are surprisingly similar across disciplines and that they are potential science policy instruments.
Molecular fossils reveal ancient associations of dsDNA viruses with several phyla of fungi
Authors: Gong Zhen , Zhang Yu , Han Guan-Zhu
Published: January 2020

Little is known about the infections of double-stranded DNA (dsDNA) viruses in fungi. Here, we use a paleovirological method to systematically identify the footprints of past dsDNA virus infections within the fungal genomes. We uncover two distinct groups of endogenous nucleocytoplasmic large DNA viruses (NCLDVs) in at least seven fungal phyla (accounting for about a third of known fungal phyla), revealing an unprecedented diversity of dsDNA viruses in fungi. Interestingly, one fungal dsDNA virus lineage infecting six fungal phyla is closely related to the giant virus Pithovirus, suggesting giant virus relatives might widely infect fungi. Co-speciation analyses indicate fungal NCLDVs mainly evolved through cross-species transmission. Taken together, our findings provide novel insights into the diversity and evolution of NCLDVs in fungi.
Cytochrome b Mutations F129L and G143A Confer Resistance to Azoxystrobin in Cercospora nicotianae, the Frogeye Leaf Spot Pathogen of Tobacco
Authors: Dixon Edward , Barlow William , Walles Grant , Amsden Bernadette , Hirsch R. Louis , Pearce Robert , Pfeufer Emily E.
Published: January 2020

Azoxystrobin is the only synthetic, systemic fungicide labeled in the United States for management of frogeye leaf spot (FLS) of tobacco (Nicotiana tabacum L.), caused by Cercospora nicotianae. Though traditionally considered a minor disease in the United States, FLS has recently become yield and quality limiting. In 2016 and 2017, 100 C. nicotianae isolates were collected from symptomatic tobacco from eight counties in Kentucky, United States. Prior to azoxystrobin sensitivity testing, some C. nicotianae isolates were found to utilize the alternative oxidase pathway and, after assay comparisons, conidial germination was utilized to evaluate sensitivity in C. nicotianae as opposed to mycelial growth. Azoxystrobin sensitivity was determined by establishing the effective concentration to inhibit 50% conidial germination (EC50) for 47 (in 2016) and 53 (in 2017) C. nicotianae isolates. Distributions of C. nicotianae EC50 values indicated three qualitative levels of sensitivity to azoxystrobin. Partial cytochrome b sequence, encompassing the F129L and G143A mutation sites, indicated single-nucleotide polymorphisms (SNPs) conferring the F129L mutation in C. nicotianae of moderate resistance (azoxystrobin at 0.177 ≤ EC50 ≤ 0.535 µg/ml) and the G143A mutation in isolates with an azoxystrobin-resistant phenotype (azoxystrobin EC50 > 1.15 µg/ml). Higher frequencies of resistant isolates were identified from greenhouse transplant (4 of 17) and conventionally produced (58 of 62) tobacco samples, as compared with field-grown tobacco (<4 weeks prior to harvest; 4 of 62) or organically produced samples (1 of 7), respectively. Together, these results suggest that resistance to azoxystrobin in C. nicotianae occurs broadly in Kentucky, and generate new hypotheses about selection pressure affecting resistance mutation frequencies.
Anthracnose Disease of Carpetgrass (Axonopus compressus) Caused by Colletotrichum hainanense sp. nov.
Authors: Zhang Wu , Damm Ulrike , Crous Pedro W. , Groenewald Johannes Z. , Niu Xueli , Lin Jinmei , Li Yuting
Published: January 2020

Carpetgrass (Axonopus compressus) is a creeping, stoloniferous, perennial warm-season grass that is adapted to humid tropical and subtropical climates. Recently, outbreaks of anthracnose disease of A. compressus caused by an unidentified Colletotrichum sp. were observed in the Hainan and Guangdong provinces in southern China. In late winter and early spring, the disease incidence reached 100% in some badly infected lawns. Under high-moisture conditions, the crowns and oldest leaf sheaths of the majority of the plants became necrotic, which led to whole lawns turning reddish brown. Pathogenicity was confirmed by inoculating uninfected A. compressus plants with a conidial suspension of the Colletotrichum sp. isolated from diseased Axonopus plants. Phylogenetic analyses of the combined internal transcribed spacer, Sod2, Apn2, and Apn2/Mat1 sequences revealed the pathogen to be a novel species of the Colletotrichum graminicola species complex. Microscopic examination showed that the species was also morphologically distinct from related Colletotrichum species. As a result of the phylogenetic, morphological, and pathogenicity analyses, we propose the name Colletotrichum hainanense for this pathogen of A. compressus in southern China.
Consideration of Latent Infections Improves the Prediction of Botrytis Bunch Rot Severity in Vineyards
Authors: Fedele Giorgia , González-Domínguez Elisa , Delière Laurent , Díez-Navajas Ana M. , Rossi Vittorio
Published: January 2020

The current study validated a mechanistic model for Botrytis cinerea on grapevine with data from 23 independent Botrytis bunch rot (BBR) epidemics (combinations of vineyards × year) that occurred between 1997 and 2018 in Italy, France, and Spain. The model was operated for each vineyard by using weather data and vine growth stages to anticipate, at any day of the vine-growing season, the disease severity (DS) at harvest (severe, DS ≥ 15%; intermediate, 5 < DS < 15%; and mild, DS ≤ 5%). To determine the ability of the model to account for latent infections, postharvest incubation assays were also conducted using mature berries without symptoms or signs of BBR. The model correctly classified the severity of 15 of 23 epidemics (65% of epidemics) when the classification was based on field assessments of BBR severity; when the model was operated to include BBR severity after incubation assays, its ability to correctly predict BBR severity increased from 65% to >87%. This result showed that the model correctly accounts for latent infections, which is important because latent infections can substantially increase DS. The model was sensitive and specific, with the false-positive and false-negative proportion of model predictions equal to 0.24 and 0, respectively. Therefore, the model may be considered a reliable tool for decision-making for BBR control in vineyards.
Management and Soil Conditions Influence Common Scab Severity on Potato Tubers Via Indirect Effects on Soil Microbial Communities
Authors: Lankau Emily W. , Xue Dianne , Christensen Rachel , Gevens Amanda J. , Lankau Richard A.
Published: January 2020

Common scab, caused by Streptomyces scabies and related species, is a potato tuber blemish disease that causes reductions in marketable yield worldwide. Evidence of suppression of common scab by indigenous soil microbial populations has been found in several studies. However, we lack a comprehensive understanding of how common scab severity relates functionally to potato varieties, farming systems, soil physical and chemical properties, and soil microbial communities. These factors may affect disease directly or indirectly by affecting one of the other variables. We performed a survey of 30 sampling locations across 12 fields in Wisconsin and used structural equation modeling to disentangle the direct effects of potato market classes, farm management (conventional versus organic), and soil physiochemical properties on common scab severity from their indirect effects mediated through soil bacterial and fungal communities. We found that, although potato market classes affected disease severity directly, the effects of farm management and soil physiochemistry were best explained as indirect, mediated by their impacts on soil bacterial communities. This suggests that evaluating the consequences of specific management practices for soil microbial communities may be useful for understanding disease pressure across fields.
Detection of Cercospora beticola and Phoma betae on Table Beet Seed using Quantitative PCR
Authors: Knight Noel L. , Koenick Lori B. , Sharma Sandeep , Pethybridge Sarah J.
Published: January 2020

Cercospora beticola and Phoma betae are important pathogens of table beet, sugar beet, and Swiss chard (Beta vulgaris subsp. vulgaris), causing Cercospora leaf spot (CLS) and Phoma leaf spot, root rot, and damping-off, respectively. Both pathogens may be seedborne; however, limited evidence is available for seed infestation by C. beticola. Due to the limitations of culture-based seed assessment methods, detection of these pathogens was investigated using PCR. A P. betae-specific quantitative PCR assay was developed and used in conjunction with a C. beticola-specific assay to assess the presence of pathogen DNA in 12 table beet seed lots. DNA of C. beticola and P. betae was detected in four and eight seed lots, respectively. Plate tests and BIO-PCR confirmed the viability of each pathogen; however, competitive growth of other microbes and low incidence limited the frequency and sensitivity of detection in some seed lots. The results for P. betae support previously described infestation of seed. Further investigation of C. beticola-infested seed lots indicated the ability of seedborne C. beticola to cause CLS on plants grown from infested seed. Detection of viable C. beticola on table beet seed demonstrates the potential for pathogen dispersal and disease initiation via infested seed, and provides valuable insight into the epidemiology of CLS. Surveys of commercial table beet seed are required to determine the frequency and source of C. beticola seed infestation and its role as primary inoculum for epidemics, and to evaluate the effectiveness of seed treatments.
Contemporary Strategies in Pilon Fixation
Authors: Hebert-Davies Jonah , Kleweno Conor P. , Nork Sean E.
Published: January 2020

The treatment of tibial pilon fractures has evolved substantially over the past decades due to ever-increasing high-energy injuries. Open reduction and internal fixation of these intra-articular fractures requires an appreciation for a number of basic principles: respect the soft tissues, understand the fracture pattern, use safe surgical approaches, and provide stability that allows for early motion of the ankle. Surgical strategy should be customized based on the fracture pattern, access needed for fracture visualization and reduction, and status of the soft tissues. Given the ability to obtain an accurate stable reduction, smaller implants are typically adequate using multiple small incisions. We view this surgical tactic as continuing the evolution of complex fracture treatment whose origins lie in the influences of pioneers such as Dr Sigvard T. Hansen Jr.
Black Race as a Social Determinant of Health and Outcomes After Lumbar Spinal Fusion Surgery
Authors: Aladdin Dima El Halawani , Tangel Virginia , Lui Briana , Pryor Kane O. , Witkin Lisa R. , White Robert S.
Published: January 2020

Study Design.

A retrospective analysis of patient hospitalization and discharge records.

Objective.

To examine the association between race and inpatient postoperative complications following lumbar spinal fusion surgery.

Summary of Background Data.

Racial disparities in healthcare have been demonstrated across a range of surgical procedures. Previous research has identified race as a social determinant of health that impacts outcomes after lumbar spinal fusion surgery. However, these studies are limited in that they are outdated, contain data from a single institution, analyze small limited samples, and report limited outcomes. Our study aims to expand and update the literature examining the association between race and inpatient postoperative complications following lumbar spine surgery.

Methods.

We analyzed 267,976 patient discharge records for inpatient lumbar spine surgery using data from the Healthcare Cost and Utilization Project's State Inpatient Databases for California, Florida, New York, Maryland, and Kentucky from 2007 through 2014. We used unadjusted bivariate analysis, adjusted multivariable, and stratified analysis to compare patient demographics, present-on-admission comorbidities, hospital characteristics, and complications by categories of race/ethnicity.

Results.

Black patients were 8% and 14% more likely than white patients to experience spine surgery specific complications (adjusted odds ratios [aOR]: 1.08, 95% confidence interval [CI]: 1.03–1.13) and general postoperative complications (aOR: 1.14, 95% CI: 1.07–1.20), respectively. Black patients, compared with white patients, also had increased adjusted odds of 30-day readmissions (aOR: 1.13, 95% CI: 1.07–1.20), 90-day readmissions (aOR: 1.07, 95% CI: 1.02–1.13), longer length of stay (LOS) (adjusted Incidence Rate Ratio: 1.15, 95% CI: 1.14–1.16), and higher total charges (adjusted Incidence Rate Ratio: 1.08, 95% CI: 1.07–1.09).

Conclusion.

Our findings demonstrate that black patients, as compared with white patients, are more likely to have postoperative complications, longer postoperative lengths of stay, higher total hospital charges, and increased odds of 30- and 90-day readmissions following lumbar spinal fusion surgery.

Level of Evidence: 4
Cardiac Rehabilitation in Frail Older Adults With Cardiovascular Disease
Authors: Flint Kelsey M. , Stevens-Lapsley Jennifer , Forman Daniel E.
Published: January 2020

Older adults with cardiovascular disease (CVD) pose challenges to cardiac rehabilitation (CR) clinicians because their disease is often coupled to physical frailty. Older patients with CVD and frailty may be less likely to tolerate conventional CR exercise training due to multidimensional (ie, strength, mobility, and balance) physical impairments. Furthermore, conventional CR typically emphasizes endurance training without addressing the intrinsic skeletal muscle impairments of frail patients that often manifest as deficits in strength, mobility, and balance, undercutting feasibility and any likely benefits. However, if appropriately modified to meet the needs of frail older adults, CR may be a powerful tool for this challenging population. To best serve frail, older adults with CVD, CR programs can incorporate well-validated strategies to assess frailty and physical function that also fit within the workflows and patient populations of individual programs. Such frailty assessments provide opportunities to identify specific targets (eg, weakness) that need to be addressed before a subsequent aerobic training program can be successfully implemented and sustained. The current review focuses on the use of physical frailty measures in older adults with CVD, with practical considerations for their clinical use in contemporary CR, as well as directions for future research.
Double-Masked and Unmasked Prospective Study of Terpinen-4-ol Lid Scrubs With Microblepharoexfoliation for the Treatment of Demodex Blepharitis
Authors: Epstein Ilan J. , Rosenberg Eric , Stuber Ross , Choi Michael B. , Donnenfeld Eric D. , Perry Henry D.
Published: January 2020

Purpose:

To evaluate terpinen-4-ol lid scrubs (Cliradex) with Microblepharoexfoliation (MBE) (BlephEx) in the treatment of Demodex blepharitis.

Methods:

An Institutional Review Board (IRB) approved the randomized prospective double-masked trial of 50 patients with Demodex positive blepharitis. All subjects were given an in-office MBE treatment and randomized to masked lid scrubs (terpinen-4-ol or sham) twice daily for 1 month. All subjects then returned for an additional MBE treatment and received open-label terpinen-4-ol scrubs twice daily for 1 month.

Outcomes:

Demodex levels, Ocular Surface Disease Index, tear osmolarity, MMP-9, Schirmer 1 and grading of the lid margin appearance, meibomian gland dropout, and meibomian gland secretions.

Results:

Forty-six subjects (23 per group) were included for the analysis; 4 lost to follow up. Total Demodex folliculorum levels in the Cliradex group improved: 4.7 to 3.6 (first month interval P = 0.266) to 2.6 (second month interval P = 0.279), overall P < 0.05 over 2 months. Total levels in the sham group improved: 5.1 to 3.0 (first month interval P < 0.05) to 2.5 (second month interval P = 0.496), overall P < 0.05 over 2 months. No clear statistically meaningful improvements in other dry eye and blepharitis data metrics were observed.

Conclusions:

In-office MBE treatments combined with either Cliradex terpinen-4-ol medicated lid scrubs or sham scrubs showed a statistically significant reduction in D. folliculorum infestation levels; however, conclusions on clinical significance could not be made. In-office MBE with terpinen-4-ol lid scrubs showed no significant improvement over sham scrubs.
Evaluation of Meibomian Glands in Obstructive Sleep Apnea-Hypopnea Syndrome
Authors: Muhafiz Ersin , Ölçen Merhamet , Erten Remzi , Bozkurt Erdinç
Published: January 2020

Purpose:

To evaluate the effect of obstructive sleep apnea-hypopnea syndrome (OSAHS) on the meibomian glands, ocular surface, and tear parameters.

Methods:

The study included 59 individuals (32 subjects with OSAHS, 27 control subjects) who underwent polysomnography in the Chest Diseases Clinic. The right eyes of all individuals were evaluated in the Ophthalmology Clinic. The first noninvasive tear break-up time (f-NTBUT) and the average noninvasive tear break-up time were measured, and Schirmer test was applied. Meibography was taken (Sirius; CSO, Florence, Italy), and meiboscore was used for indexing meibography. Finally, a sample was taken for conjunctival impression cytology.

Results:

In the study and control groups, the median of f-NTBUT was 2.1 seconds [interquartile range (IQR); 1.5–5.0] and 5.7 seconds (IQR; 2.9–8.8) and the median of average noninvasive tear break-up time was 5.6 seconds (IQR; 3.6–9.5) and 7.2 seconds (IQR; 4.2–10.7), respectively (P = 0.007 and P = 0.487, respectively). The mean Schirmer value was 16.3 ± 5.9 mm (range; 5–25) and 17.3 ± 6.6 mm (range; 5–30) in the study and control groups (P = 0.604). In upper eyelid meibography, the median of loss in the meibomian glands was 20.10% (IQR; 11.60%–40.80%) in the study group and 14.70% (IQR; 10.40%–21.30%) in the control group (P = 0.043). In lower eyelid meibography, the median of loss in the meibomian glands was 19.00% (IQR; 13.60%–35.30%) in the study group and 12.40% (IQR; 9.10%–16.40%) in the control group (P = 0.002). The median of the Nelson grade in conjunctival impression cytology was 1 (IQR; 0–1) in the study group and 1 (IQR; 0–1) in the control group (P = 0.445).

Conclusions:

The loss in the meibomian glands together with the shortened f-NTBUT shows that there could be a predisposition in OSAHS for evaporative dry eye originating from meibomian gland damage.
Intergroup anxiety in pain care: impact on treatment recommendations made by white providers for black patients
Authors: Grant Alexis D. , Miller Megan M. , Hollingshead Nicole A. , Anastas Tracy M. , Hirsh Adam T.
Published: January 2020

Race disparities in pain care are well-documented. Given that most black patients are treated by white providers, patient–provider racial discordance is one hypothesized contributor to these disparities. Research and theory suggest that providers' trait-level intergroup anxiety impacts their state-level comfort while treating patients, which, in turn, impacts their pain treatment decisions. To test these hypothesized relationships, we conducted a planned secondary analysis of data from a randomized controlled trial of a perspective-taking intervention to reduce pain treatment disparities. Mediation analyses were conducted on treatment decision data from white providers for black virtual patients with chronic pain. Results indicated that white providers with higher trait-level intergroup anxiety reported lower state-level comfort treating black patients and were thereby more likely to recommend opioid (indirect effect = 0.76, 95% confidence interval [CI]: 0.21-1.51) and pain specialty (indirect effect = 0.91, 95% CI: 0.26-1.78) treatments and less likely to recommend nonopioid analgesics (indirect effect = −0.45, 95% CI: −0.94 to −0.12). Neither trait-level intergroup anxiety nor state-level comfort significantly influenced provider decisions for physical therapy. This study provides important new information about intrapersonal and interpersonal contributors to race disparities in chronic pain care. These findings suggest that intergroup anxiety and the resulting situational discomfort encroach on the clinical decision-making process by influencing white providers' decisions about which pain treatments to recommend to black patients. Should these findings be replicated in future studies, they would support interventions to help providers become more aware of their trait-level intergroup anxiety and manage their state-level reactions to patients who are racially/ethnically different from themselves.