bluuebunny/crossref_metadata_2025 huggingface dataset:
Treatment Integrity of Interventions With Children in the Journal of Positive Behavior Interventions From 1999 to 2009
Authors: Sanetti Lisa M. Hagermoser , Dobey Lisa M. , Gritter Katie L.
Published: April 2011

For more than 10 years, the Journal of Positive Behavior Interventions has published, among other types of articles, behavioral intervention outcome studies related to positive behavior support. Operationally defining interventions is important to facilitating replication studies and adoption of intervention in applied settings. Furthermore, treatment integrity data are necessary to make valid claims that changes in outcomes resulted from intervention implementation and are thus essential to the internal validity of intervention outcome research. Reviews of treatment outcome research in related fields (e.g., applied behavior analysis) indicate that although many researchers operationally define interventions, a majority of researchers fail to report treatment integrity data. The purpose of this study was to review the treatment integrity data reported in all experimental intervention studies published in the Journal of Positive Behavior Interventions between 1999 and 2009. Results indicate that in recent years, a majority of published studies include a definition of the independent variable but do not provide quantitative treatment integrity data.
Cosmopolitan politics, security, political subjectivity
Authors: Jabri Vivienne
Published: April 2011

Conventionally, the concept of cosmopolitanism is dealt with as a normative discourse. However, a far more useful understanding is to focus on the practices that the concept enables. The aim in this article is to highlight two manifestations of such practices, namely those that have as their imperative security, the cosmopolitanism of government, and those that might be defined in terms of solidarity, the cosmopolitanism of politics. Both the socio-historical context of the rise of liberal modernity as well as its late-modern manifestations in contemporary security practices suggest that these two articulations of cosmopolitanism should not be seen in oppositional terms, but rather as being mutually implicating and mutually present. While the concept enables a government of populations, containing within it a colonial rationality, the article suggests that there is an excess to the concept that steers it beyond government through security and towards the politics of solidarity. Placing the lens on the forms of political subjectivity generated through cosmopolitan practices, the article highlights the concept’s potential in revealing the political implications of contemporary practices that have the postcolonial world as the primary target of their operations.
Positions and salience in European Union politics: Estimation and validation of a new dataset
Authors: Veen Tim
Published: April 2011

This article introduces the ‘Positions and Salience in European Union Politics’ dataset. The dataset comprises positional and salience estimates of more than 250 parties and governments in the European Union (EU). These estimates, which all come with measures of uncertainty, pertain to 10 important EU policy domains as well as a European integration and a left—right scale. The dataset exploits statistics from hand-coded European party manifestos provided by the ‘Euromanifestos’ project and uses simulation to correct stochastic error. The manifestos are scaled using a technique for count data that employs principles from psychophysics. For most European domestic parties and major European Parliament groups, the estimates range from 1979 to 2004, while for member state governments time-series between 1998 and 2007 are available. The dataset may be of use to scholars interested in European integration, Europeanization, compliance research or EU legislative decision-making.
Mapping EU attitudes: Conceptual and empirical dimensions of Euroscepticism and EU support
Authors: Boomgaarden Hajo G. , Schuck Andreas R. T. , Elenbaas Matthijs , de Vreese Claes H.
Published: April 2011

Public attitudes towards the European Union (EU) are at the heart of a growing body of research. The nature, structure and antecedents of these attitudes, however, are in need of conceptual and empirical refinement. With growing diversification of the policies of the Union, a one-dimensional approach to attitudes towards the EU may be insufficient. This study reviews existing approaches towards theorizing EU public opinion. Based on this inventory, originally collected public opinion survey data (n = 1394) indicate the presence of five dimensions of EU attitudes: performance, identity, affection, utilitarianism and strengthening. The study furthermore shows that different predictors of EU public opinion matter to differing extents when explaining these dimensions. In light of these findings, we suggest tightening the link, conceptually and empirically, between attitudinal dimensions and their antecedents.
The European Union Policy-Making dataset
Authors: Häge Frank M.
Published: April 2011

This article introduces the European Union Policy - Making (EUPOL) dataset. The dataset contains the complete records of the European Commission’s PreLex database, which tracks the interactions between the European institutions on legislative proposals and non-legislative policy documents over time. To be of maximum use to the research community, the dataset is both comprehensive and replicable. It relies on 2600 variables to describe the detailed event histories of more than 29,000 inter-institutional decision-making processes between 1975 and 2009. The data collection has been completely automated, enabling scholars to scrutinize and replicate the generation of the dataset. To illustrate the dataset’s general utility and discuss specific pitfalls, I present a descriptive analysis of the outcome and duration of Council decision-making.
The NBA Draft
Authors: Arel Barbara , Tomas Michael J.
Published: April 2011

The National Basketball Association (NBA) has a fixed salary schedule that allows us to examine this labor market to understand the incentives for players to enter the draft. Previous studies have focused on labor as a choice from the team’s perspective. Early entry into the NBA draft is similar to the decision to exercise an American style put option early; the draftee sells his remaining time in college early. Using mock draft and actual draft data, the authors find early exercise to be rational for all class ranks; though the early exercise boundary is considerably different for freshmen and juniors.
Filial Responsibility Expectations Among Mexican American Undergraduates: Gender and Biculturalism
Authors: Rudolph Bonnie , Chavez Mary , Quintana Fernando , Salinas Gilberto
Published: April 2011

How Mexican American college students perceive responsibility for parental care is important as Mexican American elders’ numbers increase. The authors applied mixed methods to investigate the impact of gender and biculturalism within this group. Two hundred and eighty-six Mexican American undergraduates completed the Hamon Filial Responsibility Scale. Twenty others participated in focus groups. Females endorsed filial responsibility expectations at higher rates than males, but biculturalism influenced more change in elder care expectations among males.
Exploring Factors of a Web-Based Seminar That Influence Hispanic Preservice Teachers’ Critical Thinking and Problem-Solving Skills
Authors: Garcia Criselda G. , Hooper H.H.
Published: April 2011

The purpose of the qualitative study using a phenomenological approach was to gain insight of preservice teachers’ experiences with a WebCT seminar designed to develop critical thinking and problem-solving skills in a Hispanic-Serving Institution’s teacher education program. By applying a “holistic approach” to analyze data, NVivo software was used to assist the process. Findings indicate the use of discussion board and a reading strategy were influential in developing critical thinking and problem-solving skills, respectively.
Indicators of Persistence for Hispanic Undergraduate Achievement: Toward an Ecological Model
Authors: Arana Renelinda , Castañeda-Sound Carrie , Blanchard Steve , Aguilar Teresita E.
Published: April 2011

By examining Hispanic students both currently and formerly enrolled at a private, Hispanic-serving Institution located in the Southwestern region of the United States, this study attempts to understand the factors that lead to Hispanic undergraduate persistence to graduation. Adapting Bronfenbrenner’s theoretical approach, this study explores three dimensions that are critical to understanding Hispanic undergraduate persistence to graduation: (a) the role of the student context (b) the role of the university context in the student’s decision to persist; and (c) the interaction between the two contexts.
The College Assistance Migrant Program: A Valuable Resource for Migrant Farmworker Students
Authors: Araujo Blanca
Published: April 2011

Using community cultural wealth through the lens of Latino critical race theory, this article will focus on the experiences told by eight Latino migrant farmworkers students about their first year of college. It describes how the College Assistance Migrant Program (CAMP) was influential in providing the students with different forms of capital by helping them with financial, informational, emotional, and academic assistance.
Vitamin D Deficiency in HIV-Infected Women on Antiretroviral Therapy Living in the Tropics
Authors: Conrado Tereza , Miranda-Filho Demócrito de Barros , Ximenes Ricardo Arraes de Alencar , Albuquerque Maria de Fátima , Lacerda Heloísa Ramos , Ramos Regina Coeli F. , Araújo Paulo Sérgio Ramos de , Montarroyos Ulisses , Bandeira Francisco
Published: April 2011

The effects of HIV/AIDS and antiretroviral drugs on vitamin D metabolism are still mostly unknown. This was a cross-sectional study to estimate the prevalence of vitamin D deficiency and identify its association with the clinical and metabolic parameters among 214 HIV-positive female patients on antiretroviral therapy (ART) in Brazil. The prevalence of vitamin D deficiency (< 30 ng/ml) was 40.65% (87/214). Hypercholesterolemia, high LDL-c, duration of use of current antiretroviral regimen, hypertriglyceridemia, body mass index, age, hypertension, time with AIDS ≥ 10 years and hyperglycemia were selected for multivariate analysis (p < 0.20). After this analysis, hypercholesterolemia and use of current antiretroviral regimen ≥ 3 years remained independently associated with vitamin D deficiency. There was an inverse statistically significant correlation between total cholesterol and serum 25(OH)D levels. High prevalence of vitamin D deficiency was found among HIV-positive women on ART and was independently associated with its prolonged use and with hypercholesterolemia.
A Comparison of Sonographic Assessments and Clinical Questionnaire in the Diagnosis of HIV-Associated Lipodystrophy
Authors: Signorini Dario José Hart Pontes , Netto Ana Maria Schmidt de Oliveira , Gabbay Sergio , Monteiro Michelle Carreira Miranda , Signorini Dario Hart , Andrade Marion de Fátima Castro de , Bastos Francisco I. , Codeço Cláudia Torres
Published: April 2011

The study evaluated the use of sonographic measurements as an alternative to assessments based on clinical or other imaging techniques for the diagnosis of body-fat abnormalities. The study enrolled 179 HIV-infected patients, 81 (45.3%) of them diagnosed as lipodystrophy (LD)-positive based on a clinical standard questionnaire. Association between clinical LD and sonographic measurements of face, right upper limb, subcutaneous abdomen, and visceral compartments was evaluated by multiple logistic regression. The predicted probability of the logistic model was 0.64, corresponding to a maximum sensitivity of 69.1% (58%-79%), a specificity of 94.9% (88%-98%), and to positive and negative predictive values of 92% (82%-97%) and 79% (70%-86%), respectively. Kappa measure of concordance was 65% (54%-77%). Low sensitivity poses a problem for the use of sonography to detect LD in the clinical routine as a single exam, speaking in favor of the combined use of clinical and sonographic measurements over time.
Emergency Transumbilical Single-Incision Laparoscopic Splenectomy for the Treatment of Traumatic Rupture of the Spleen: Report of the First Case and Literature Review
Authors: Fan Ying  , Wu Shuo-Dong , Ernest-Amos Siwo
Published: April 2011

Aim. The authors report 1 case of emergency splenectomy for the treatment of traumatic rupture of the spleen where a laparoendoscopic single-site surgery technique was performed. Method. A 17-year-old male with a diagnosis of traumatic rupture of the spleen underwent emergency transumbilical single-incision laparoscopic splenectomy. Three ports, including 5 mm and 10 mm ports, were placed through a transumbilical incision for the procedure. Results. Intraoperative and postoperative courses were uneventful. The patient was discharged home 8 days after the surgery in a stable condition. Discussion. The Laparoendoscopic single site surgery technique is still in its infancy, but because of its reduced invasion and ideal aesthetic results, it is well received by patients. Improvements are needed to widen its application. Conclusion. To the authors’ knowledge, this is the first report where an emergency laparoendoscopic single site surgery technique has been used in the management of a ruptured spleen.
Beyond folk devil resistance: Linking moral panic and moral regulation
Authors: Hier Sean P. , Lett Dan , Walby Kevin , Smith André
Published: April 2011

This article is a contribution to widening the focus of moral panic studies. Our aim is to advance recent attempts to link moral panic studies to the criminological literature on moral regulation. We argue that moral panics should be conceptualized as volatile expressions of long-term moral regulation processes. To substantiate these conceptual and theoretical arguments, we examine claims-making activities about the threat posed by British youth who don hooded tops in public places.
Pretreatment with phosphatase and tensin homolog deleted on chromosome 10 (PTEN) inhibitor SF1670 augments the efficacy of granulocyte transfusion in a clinically relevant mouse model
Authors: Li Yitang , Prasad Amit , Jia Yonghui , Roy Saurabh Ghosh , Loison Fabien , Mondal Subhanjan , Kocjan Paulina , Silberstein Leslie E. , Ding Sheng , Luo Hongbo R.
Published: April 2011

The clinical outcome of granulocyte transfusion therapy is often hampered by short ex vivo shelf life, inefficiency of recruitment to sites of inflammation, and poor pathogen-killing capability of transplanted neutrophils. Here, using a recently developed mouse granulocyte transfusion model, we revealed that the efficacy of granulocyte transfusion can be significantly increased by elevating intracellular phosphatidylinositol (3,4,5)-trisphosphate signaling with a specific phosphatase and tensin homolog deleted on chromosome 10 (PTEN) inhibitor SF1670. Neutrophils treated with SF1670 were much sensitive to chemoattractant stimulation. Neutrophil functions, such as phagocytosis, oxidative burst, polarization, and chemotaxis, were augmented after SF1670 treatment. The recruitment of SF1670-pretreated transfused neutrophils to the inflamed peritoneal cavity and lungs was significantly elevated. In addition, transfusion with SF1670-treated neutrophils led to augmented bacteria-killing capability (decreased bacterial burden) in neutropenic recipient mice in both peritonitis and bacterial pneumonia. Consequently, this alleviated the severity of and decreased the mortality of neutropenia-related pneumonia. Together, these observations demonstrate that the innate immune responses can be enhanced and the severity of neutropenia-related infection can be alleviated by augmenting phosphatidylinositol (3,4,5)-trisphosphate in transfused neutrophils with PTEN inhibitor SF1670, providing a therapeutic strategy for improving the efficacy of granulocyte transfusion.
Inhibition of β-TrcP–dependent ubiquitination of p53 by HIV-1 Vpu promotes p53–mediated apoptosis in human T cells
Authors: Verma Sachin , Ali Amjad , Arora Sakshi , Banerjea Akhil C.
Published: April 2011

HIV-1 viral protein U (Vpu) is involved in ubiquitination and degradation of BM stromal cell Ag 2 and surface receptor CD4 through their recruitment to SCFβ-TrcP (Skp1/Cul1/F-box) ubiquitin ligase (SCF) complex. Here, we show that specific interaction of wild-type Vpu protein with SCF complex leads to inhibition of ubiquitination and proteasomal degradation of p53 protein in a β-TrcP–dependent manner. Successful interaction of SCFβ-TrcP complex with β-TrcP binding motif (DS52GNES56) present in Vpu is essential because mutant Vpu possessing specific alanine substitutions (DA52GNEA56) in the β-TrcP binding motif not only failed to stabilize p53 protein but was also unable to inhibit ubiquitination of p53 protein. Furthermore, Vpu competes efficiently with the interaction of p53 protein with the β-TrcP subunit of the SCF complex and inhibits subsequent ubiquitination of p53 proteins in a dose-dependent manner. We also observed potent apoptotic activity in a p53 null cell line (H-1299) that was cotransfected with p53 and Vpu-expressing plasmids. Furthermore, MOLT-3 (human T-lymphoblast) cells when infected with vesicular stomatitis virus glycoprotein–pseudotypic HIV-1 possessing wild-type vpu gene exhibited maximum activation of p53/Bax proteins and p53-mediated cell death. These findings establish a novel function of Vpu in modulating the stability of p53 protein that correlates positively with apoptosis during late stages of HIV-1 infection.
Prevention of bronchial hyperreactivity in a rat model of precapillary pulmonary hypertension
Authors: Habre Walid , Albu Gergely , Janosi Tibor Z , Fontao Fabienne , von Ungern-Sternberg Britta S , Beghetti Maurice , Petak Ferenc
Published: April 2011

Background

The development of bronchial hyperreactivity (BHR) subsequent to precapillary pulmonary hypertension (PHT) was prevented by acting on the major signalling pathways (endothelin, nitric oxide, vasoactive intestine peptide (VIP) and prostacyclin) involved in the control of the pulmonary vascular and bronchial tones.

Methods

Five groups of rats underwent surgery to prepare an aorta-caval shunt (ACS) to induce sustained precapillary PHT for 4 weeks. During this period, no treatment was applied in one group (ACS controls), while the other groups were pretreated with VIP, iloprost, tezosentan via an intraperitoneally implemented osmotic pump, or by orally administered sildenafil. An additional group underwent sham surgery. Four weeks later, the lung responsiveness to increasing doses of an intravenous infusion of methacholine (2, 4, 8 12 and 24 μg/kg/min) was determined by using the forced oscillation technique to assess the airway resistance (Raw).

Results

BHR developed in the untreated rats, as reflected by a significant decrease in ED50, the equivalent dose of methacholine required to cause a 50% increase in Raw. All drugs tested prevented the development of BHR, iloprost being the most effective in reducing both the systolic pulmonary arterial pressure (Ppa; 28%, p = 0.035) and BHR (ED50= 9.9 ± 1.7 vs. 43 ± 11 μg/kg in ACS control and iloprost-treated rats, respectively, p = 0.008). Significant correlations were found between the levels of Ppa and ED50(R = -0.59, p = 0.016), indicating that mechanical interdependence is primarily responsible for the development of BHR.

Conclusions

The efficiency of such treatment demonstrates that re-establishment of the balance of constrictor/dilator mediators via various signalling pathways involved in PHT is of potential benefit for the avoidance of the development of BHR.
Stochastic sequence-level model of coupled transcription and translation in prokaryotes
Authors: Mäkelä Jarno , Lloyd-Price Jason , Yli-Harja Olli , Ribeiro Andre S
Published: April 2011

Background

In prokaryotes, transcription and translation are dynamically coupled, as the latter starts before the former is complete. Also, from one transcript, several translation events occur in parallel. To study how events in transcription elongation affect translation elongation and fluctuations in protein levels, we propose a delayed stochastic model of prokaryotic transcription and translation at the nucleotide and codon level that includes the promoter open complex formation and alternative pathways to elongation, namely pausing, arrests, editing, pyrophosphorolysis, RNA polymerase traffic, and premature termination. Stepwise translation can start after the ribosome binding site is formed and accounts for variable codon translation rates, ribosome traffic, back-translocation, drop-off, and trans-translation.

Results

First, we show that the model accurately matches measurements of sequence-dependent translation elongation dynamics. Next, we characterize the degree of coupling between fluctuations in RNA and protein levels, and its dependence on the rates of transcription and translation initiation. Finally, modeling sequence-specific transcriptional pauses, we find that these affect protein noise levels.

Conclusions

For parameter values within realistic intervals, transcription and translation are found to be tightly coupled in Escherichia coli, as the noise in protein levels is mostly determined by the underlying noise in RNA levels. Sequence-dependent events in transcription elongation, e.g. pauses, are found to cause tangible effects in the degree of fluctuations in protein levels.
Reversal to air-driven sound production revealed by a molecular phylogeny of tongueless frogs, family Pipidae
Authors: Irisarri Iker , Vences Miguel , San Mauro Diego , Glaw Frank , Zardoya Rafael
Published: April 2011

Background

Evolutionary novelties often appear by conferring completely new functions to pre-existing structures or by innovating the mechanism through which a particular function is performed. Sound production plays a central role in the behavior of frogs, which use their calls to delimit territories and attract mates. Therefore, frogs have evolved complex vocal structures capable of producing a wide variety of advertising sounds. It is generally acknowledged that most frogs call by moving an air column from the lungs through the glottis with the remarkable exception of the family Pipidae, whose members share a highly specialized sound production mechanism independent of air movement.

Results

Here, we performed behavioral observations in the poorly known African pipid genusPseudhymenochirusand document that the sound production in this aquatic frog is almost certainly air-driven. However, morphological comparisons revealed an indisputable pipid nature ofPseudhymenochiruslarynx. To place this paradoxical pattern into an evolutionary framework, we reconstructed robust molecular phylogenies of pipids based on complete mitochondrial genomes and nine nuclear protein-coding genes that coincided in placingPseudhymenochirusnested among other pipids.

Conclusions

We conclude that althoughPseudhymenochirusprobably has evolved a reversal to the ancestral non-pipid condition of air-driven sound production, the mechanism through which it occurs is an evolutionary innovation based on the derived larynx of pipids. This strengthens the idea that evolutionary solutions to functional problems often emerge based on previous structures, and for this reason, innovations largely depend on possibilities and constraints predefined by the particular history of each lineage.
Empirical evaluations of analytical issues arising from predicting HLA alleles using multiple SNPs
Authors: Zhang Xinyi Cindy , Li Shuying Sue , Wang Hongwei , Hansen John A , Zhao Lue Ping
Published: April 2011

Background

Numerous immune-mediated diseases have been associated with the class I and II HLA genes located within the major histocompatibility complex (MHC) consisting of highly polymorphic alleles encoded by the HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 loci. Genotyping for HLA alleles is complex and relatively expensive. Recent studies have demonstrated the feasibility of predicting HLA alleles, using MHC SNPs inside and outside of HLA that are typically included in SNP arrays and are commonly available in genome-wide association studies (GWAS). We have recently described a novel method that is complementary to the previous methods, for accurately predicting HLA alleles using unphased flanking SNPs genotypes. In this manuscript, we address several practical issues relevant to the application of this methodology.

Results

Applying this new methodology to three large independent study cohorts, we have evaluated the performance of the predictive models in ethnically diverse populations. Specifically, we have found that utilizing imputed in addition to genotyped SNPs generally yields comparable if not better performance in prediction accuracies. Our evaluation also supports the idea that predictive models trained on one population are transferable to other populations of the same ethnicity. Further, when the training set includes multi-ethnic populations, the resulting models are reliable and perform well for the same subpopulations across all HLA genes. In contrast, the predictive models built from single ethnic populations have superior performance within the same ethnic population, but are not likely to perform well in other ethnic populations.

Conclusions

The empirical explorations reported here provide further evidence in support of the application of this approach for predicting HLA alleles with GWAS-derived SNP data. Utilizing all available samples, we have built "state of the art" predictive models for HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1. The HLA allele predictive models, along with the program used to carry out the prediction, are available on our website.
Identification of ovule transcripts from the Apospory-Specific Genomic Region (ASGR)-carrier chromosome
Authors: Zeng Yajuan , Conner Joann , Ozias-Akins Peggy
Published: April 2011

Background

Apomixis, asexual seed production in plants, holds great potential for agriculture as a means to fix hybrid vigor. Apospory is a form of apomixis where the embryo develops from an unreduced egg that is derived from a somatic nucellar cell, the aposporous initial, via mitosis. Understanding the molecular mechanism regulating aposporous initial specification will be a critical step toward elucidation of apomixis and also provide insight into developmental regulation and downstream signaling that results in apomixis. To discover candidate transcripts for regulating aposporous initial specification in P. squamulatum, we compared two transcriptomes derived from microdissected ovules at the stage of aposporous initial formation between the apomictic donor parent, P. squamulatum (accession PS26), and an apomictic derived backcross 8 (BC8) line containing only the Apospory-Specific Genomic Region (ASGR)-carrier chromosome from P. squamulatum. Toward this end, two transcriptomes derived from ovules of an apomictic donor parent and its apomictic backcross derivative at the stage of apospory initiation, were sequenced using 454-FLX technology.

Results

Using 454-FLX technology, we generated 332,567 reads with an average read length of 147 base pairs (bp) for the PS26 ovule transcriptome library and 363,637 reads with an average read length of 142 bp for the BC8 ovule transcriptome library. A total of 33,977 contigs from the PS26 ovule transcriptome library and 26,576 contigs from the BC8 ovule transcriptome library were assembled using the Multifunctional Inertial Reference Assembly program. Using stringent in silico parameters, 61 transcripts were predicted to map to the ASGR-carrier chromosome, of which 49 transcripts were verified as ASGR-carrier chromosome specific. One of the alien expressed genes could be assigned as tightly linked to the ASGR by screening of apomictic and sexual F1s. Only one transcript, which did not map to the ASGR, showed expression primarily in reproductive tissue.

Conclusions

Our results suggest that a strategy of comparative sequencing of transcriptomes between donor parent and backcross lines containing an alien chromosome of interest can be an efficient method of identifying transcripts derived from an alien chromosome in a chromosome addition line.
Introgression of Swertia mussotii gene into Bupleurum scorzonerifoliumvia somatic hybridization
Authors: Wang Junfeng , Zhao Cuizhu , Liu Chang , Xia Guangmin , Xiang Fengning
Published: April 2011

Background

The wild herb Swertia mussotii is a source of the anti-hepatitis compounds swertiamarin, mangiferin and gentiopicroside. Its over-exploitation has raised the priority of producing these compounds heterologously. Somatic hybridization represents a novel approach for introgressing Swertia mussotii genes into a less endangered species.

Results

Protoplasts derived from calli of Bupleurum scorzonerifolium and S. mussotii were fused to produce 194 putative hybrid cell lines, of which three (all derived from fusions where the S. mussotii protoplasts were pre-treated for 30 s with UV light) later differentiated into green plants. The hybridity of the calli was confirmed by a combination of isozyme, RAPD and chromosomal analysis. The hybrid calli genomes were predominantly B. scorzonerifolium. GISH analysis of mitotic chromosomes confirmed that the irradiation of donor protoplasts increased the frequency of chromosome elimination and fragmentation. RFLP analysis of organellar DNA revealed that mitochondrial and chloroplast DNA of both parents coexisted and recombined in some hybrid cell lines. Some of the hybrid calli contained SmG10H from donor, and produced swertiamarin, mangiferin and certain volatile compounds characteristic of S. mussotii. The expression of SmG10H (geraniol 10-hydroxylase) was associated with the heterologous accumulation of swertiamarin.

Conclusions

Somatic hybrids between B. scorzonerifolium and S. mussotii were obtained, hybrids selected all contained introgressed nuclear and cytoplasmic DNA from S. mussotii; and some produced more mangiferin than the donor itself. The introgression of SmG10H was necessary for the accumulation of swertiamarin.
Performance of whole-blood interferon-gamma release assay in patients admitted to the emergency department with pulmonary infiltrates
Authors: Lee Yoon Jee , Lee Jaehee , Kim Yi Young , Won Dong Il , Cha Seung Ick , Park Jae Yong , Jung Tae Hoon , Kim Chang Ho
Published: April 2011

Background

This study was conducted to evaluate the performance of a whole-blood interferon-gamma release assay in inpatients who were admitted to the emergency department (ED) with pulmonary infiltrates who required a differential diagnosis with pulmonary tuberculosis (TB).

Methods

The patients with pulmonary infiltrates who received a QuantiFERON (QFT) test in the ED were included as an inpatient group and were divided into TB and non-TB group based on the final diagnosis. Patients with pulmonary TB who were tested in the outpatient department served as a control group.

Results

In total, 377 QFT tests were analyzed. Of the 284 inpatient QFT tests, 29.6% had an indeterminate result (35.2% in the 196 patients with non-TB and 17.0% in the 88 patients with TB). In contrast, only 1.1% of the 93 outpatients with TB returned an indeterminate result (p < 0.001). The indeterminate QFT results in the inpatient group were independently associated with lymphocytopenia, hypoalbuminemia, and high C-reactive protein levels. Non-positive QFT results in inpatients with TB were associated with lymphocytopenia and hypoalbuminemia, while non-positive QFT results in outpatients with TB were associated with high erythrocyte sedimentation rates and radiographically more severe diseases.

Conclusions

QFT tests in ED-based inpatients with pulmonary infiltrate return indeterminate results relatively frequently. In addition, inpatients and outpatients with pulmonary TB may differ in terms of the risk factors on non-positive QFT results.
Activities of colistin- and minocycline-based combinations against extensive drug resistant Acinetobacter baumannii isolates from intensive care unit patients
Authors: Liang Wang , Liu Xiao-fang , Huang Jun , Zhu De-mei , Li Jian , Zhang Jing
Published: April 2011

Background

Extensive drug resistance of Acinetobacter baumannii is a serious problem in the clinical setting. It is therefore important to find active antibiotic combinations that could be effective in the treatment of infections caused by this problematic 'superbug'. In this study, we analyzed the in vitro activities of three colistin-based combinations and a minocycline-based combination against clinically isolated extensive drug resistant Acinetobacter baumannii (XDR-AB) strains.

Methods

Fourteen XDR-AB clinical isolates were collected. The clonotypes were determined by polymerase chain reaction-based fingerprinting. Susceptibility testing was carried out according to the standards of the Clinical and Laboratory Standards Institute. Activities of drug combinations were investigated against four selected strains and analyzed by mean survival time over 12 hours (MST12 h) in a time-kill study.

Results

The time-kill studies indicated that the minimum inhibitory concentration (MIC) of colistin (0.5 or 0.25 μg/mL) completely killed all strains at 2 to 4 hours, but 0.5×MIC colistin showed no bactericidal activity. Meropenem (8 μg/mL), minocycline (1 μg/mL) or rifampicin (0.06 μg/mL) did not show bactericidal activity. However, combinations of colistin at 0.5×MIC (0.25 or 0.125 μg/mL) with each of the above were synergistic and shown bactericidal activities against all test isolates. A combination of meropenem (16 μg/mL) with minocycline (0.5×MIC, 4 or 2 μg/mL) was synergitic to all test isolates, but neither showed bactericidal activity alone. The MST12 h values of drug combinations (either colistin- or minocycline-based combinations) were significantly shorter than those of the single drugs (p < 0.01).

Conclusions

This study indicates that combinations of colistin/meropenem, colistin/rifampicin, colistin/minocycline and minocycline/meropenem are synergistic in vitro against XDR-AB strains.
Evaluation of variants in the selectin genes in age-related macular degeneration
Authors: Mullins Robert F , Skeie Jessica M , Folk James C , Solivan-Timpe Frances M , Oetting Thomas A , Huang Jian , Wang Kai , Stone Edwin M , Fingert John H
Published: April 2011

Background

Age-related macular degeneration (AMD) is a common disease of the elderly that leads to loss of the central visual field due to atrophic or neovascular events. Evidence from human eyes and animal models suggests an important role for macrophages and endothelial cell activation in the pathogenesis of AMD. We sought to determine whether common ancestral variants in genes encoding the selectin family of proteins are associated with AMD.

Methods

Expression of E-selectin, L-selectin and P-selectin was examined in choroid and retina by quantitative PCR and immunofluorescence. Samples from patients with AMD (n = 341) and controls (n = 400) were genotyped at a total of 34 SNPs in the SELE, SELL and SELP genes. Allele and genotype frequencies at these SNPs were compared between AMD patients and controls as well as between subtypes of AMD (dry, geographic atrophy, and wet) and controls.

Results

High expression of all three selectin genes was observed in the choroid as compared to the retina. Some selectin labeling of retinal microglia, drusen cores and the choroidal vasculature was observed. In the genetic screen of AMD versus controls, no positive associations were observed for SELE or SELL. One SNP in SELP (rs3917751) produced p-values < 0.05 (uncorrected for multiple measures). In the subtype analyses, 6 SNPs (one in SELE, two in SELL, and three in SELP) produced p-values < 0.05. However, when adjusted for multiple measures with a Bonferroni correction, only one SNP in SELP (rs3917751) produced a statistically significant p-value (p = 0.0029).

Conclusions

This genetic screen did not detect any SNPs that were highly associated with AMD affection status overall. However, subtype analysis showed that a single SNP located within an intron of SELP (rs3917751) is statistically associated with dry AMD in our cohort. Future studies with additional cohorts and functional assays will clarify the biological significance of this discovery. Based on our findings, it is unlikely that common ancestral variants in the other selectin genes (SELE and SELL) are risk factors for AMD. Finally, it remains possible that sporadic or rare mutations in SELE, SELL, or SELP have a role in the pathogenesis of AMD.
Simvastatin suppresses dexamethasone-induced secretion of plasminogen activator inhibitor-1 in human bone marrow adipocytes
Authors: Sakamoto Kazutaka , Osaki Makoto , Hozumi Akira , Goto Hisataka , Fukushima Tatsuya , Baba Hideo , Shindo Hiroyuki
Published: April 2011

Background

Osteonecrosis of the femoral head is a common complication of high-dose glucocorticoid treatment. Intravascular thrombosis is thought to be associated with the ischemic state of the femoral head. Plasminogen activator inhibitor-1 (PAI-1) is an adipokine, which are physiologically active substances secreted from visceral and subcutaneous adipocytes. PAI-1 suppresses fibrinolysis by binding tissue-type plasminogen activator. Several reports have described the relationship between PAI-1 and steroid-induced osteonecrosis of the femoral head, and the preventive effects of lipid-lowering agents (statins) against steroid-induced osteonecrosis of the femoral head. We previously reported that adipokines and dexamethasone induced PAI-1 secretion from bone marrow adipocytes. The purpose of the present study is to examine the effects of simvastatin on PAI-1 secretion from human bone marrow adipocytes in vitro.

Methods

Primary bone marrow adipocytes were extracted from collagenase-treated bone marrow fluid obtained from the femoral necks of 40 patients (6 men, 34 women; age range, 52-81 years) undergoing hip joint replacement surgery. After suspended culture with or without dexamethasone or simvastatin, PAI-1 mRNA expression was assessed by real-time RT-PCR. Total PAI-1 protein secretion in culture medium was assessed by enzyme-linked immunosorbent assay.

Results

PAI-1 mRNA expression was up-regulated by 388% (P= 0.002) with dexamethasone, and down-regulated by 45% (P= 0.002) with simvastatin, as compared to control levels. Dexamethasone increased total PAI-1 secretion by 166% (P= 0.001) and simvastatin decreased total PAI-1 secretion by 64% (P= 0.002). No significant changes were observed in adiponectin mRNA expression and secretion by dexamethasone and simvastatin, while pre-treatment with simvastatin reversed dexamethasone induced PAI-1 secretion by 89%, as compared to control levels.

Conclusion

The present study confirmed the suppressive effects of simvastatin on PAI-1 expression and secretion from bone marrow adipocytes. Furthermore, pre-treatment with simvastatin reversed dexamethasone induced PAI-1 secretion. Simvastatin may thus exhibit preventive effects against steroid-induced osteonecrosis of the femoral head by suppressing PAI-1 secretion.
Expression of human amyloid precursor protein in the skeletal muscles of Drosophila results in age- and activity-dependent muscle weakness
Authors: Kim Chul , Srivastava Sapeckshita , Rice Marian , Godenschwege Tanja A , Bentley Brooke , Ravi Saranya , Shao Shuang , Woodard Craig T , Schwartz Lawrence M
Published: April 2011

Background

One of the hallmarks of Alzheimer's disease, and several other degenerative disorders such as Inclusion Body Myositis, is the abnormal accumulation of amyloid precursor protein (APP) and its proteolytic amyloid peptides. To better understand the pathological consequences of inappropriate APP expression on developing tissues, we generated transgenic flies that express wild-type human APP in the skeletal muscles, and then performed anatomical, electrophysiological, and behavioral analysis of the adults.

Results

We observed that neither muscle development nor animal longevity was compromised in these transgenic animals. However, human APP expressing adults developed age-dependent defects in both climbing and flying. We could advance or retard the onset of symptoms by rearing animals in vials with different surface properties, suggesting that human APP expression-mediated behavioral defects are influenced by muscle activity. Muscles from transgenic animals did not display protein aggregates or structural abnormalities at the light or transmission electron microscopic levels. In agreement with genetic studies performed with developing mammalian myoblasts, we observed that co-expression of the ubiquitin E3 ligase Parkin could ameliorate human APP-induced defects.

Conclusions

These data suggest that: 1) ectopic expression of human APP in fruit flies leads to age- and activity-dependent behavioral defects without overt changes to muscle development or structure; 2) environmental influences can greatly alter the phenotypic consequences of human APP toxicity; and 3) genetic modifiers of APP-induced pathology can be identified and analyzed in this model.
Lactococcus lactis M4, a potential host for the expression of heterologous proteins
Authors: Noreen Nanyan , Hooi Wei Yeng , Baradaran Ali , Rosfarizan Mohamad , Sieo Chin Chin , Rosli Md Illias , Yusoff Khatijah , Raha Abdul Rahim
Published: April 2011

Background

Many plasmid-harbouring strains of Lactococcus lactis have been isolated from milk and other sources. Plasmids of Lactococcus have been shown to harbour antibiotic resistance genes and those that express some important proteins. The generally regarded as safe (GRAS) status of L. lactis also makes it an attractive host for the production of proteins that are beneficial in numerous applications such as the production of biopharmaceutical and nutraceutical. In the present work, strains of L. lactis were isolated from cow's milk, plasmids were isolated and characterised and one of the strains was identified as a potential new lactococcal host for the expression of heterologous proteins.

Results

Several bacterial strains were isolated from cow's milk and eight of those were identified as Lactococcus lactis by 16S rRNA sequence analysis. Antibiotic susceptibility tests that were carried out showed that 50% of the isolates had almost identical antibiotic resistance patterns compared to the control strains MG1363 and ATCC 11454. Plasmid profiling results indicated the lack of low molecular weight plasmids for strain M4. Competent L. lactis M4 and MG1363 were prepared and electrotransformed with several lactococcal plasmids such as pMG36e, pAR1411, pAJ01 and pMG36e-GFP. Plasmid isolation and RE analyses showed the presence of these plasmids in both M4 and the control strain after several generations, indicating the ability of M4 to maintain heterologous plasmids. SDS-PAGE and Western blot analyses also confirmed the presence of GFP, demonstrating the potential of heterologous protein expression in M4.

Conclusions

Based on the 16S rRNA gene molecular analysis, eight Gram-positive cocci milk isolates were identified as L. lactis subsp. lactis. One of the strains, L. lactis M4 was able to maintain transformed low molecular weight plasmid vectors and expressed the GFP gene. This strain has the potential to be developed into a new lactococcal host for the expression of heterologous proteins.
Antiangiogenic properties of Koetjapic acid, a natural triterpene isolated from Sandoricum koetjaoe Merr
Authors: Nassar Zeyad D , Aisha Abdalrahim FA , Ahamed Mohamed BK , Ismail Zhari , Abu-Salah Khalid M , Alrokayan Salman A , Abdul Majid Amin Malik Shah
Published: April 2011

Background

Angiogenesis, the formation of new blood vessels, has become an important target in cancer therapy. Angiogenesis plays an important role in tumor growth and metastasis. Koetjapic acid (KA) is a seco-A-ring oleanene triterpene isolated from S. koetjape. The solvent extract of this plant species was shown previously to have strong antiangiogenic activity; however the active ingredient(s) that conferred the biological activity and the mode of action was not established. Given the high concentration of KA in S. koetjape, an attempt has been made in this study to investigate the antiangiogenic properties of KA.

Results

Treatment with 10-50 μg/ml KA resulted in dose dependent inhibition of new blood vessels growth in ex vivo rat aortic ring assay. KA was found to be non-cytotoxic against HUVECs with IC50 40.97 ± 0.37 μg/ml. KA inhibited major angiogenesis process steps, endothelial cell migration and differentiation as well as VEGF expression.

Conclusions

The non-cytotoxic compound, KA, may be a potent antiangiogenic agent; its activity may be attributed to inhibition of endothelial cells migration and differentiation as well VEGF suppression.
Parasitostatic effect of maslinic acid. II. Survival increase and immune protection in lethal Plasmodium yoelii-infected mice
Authors: Moneriz Carlos , Marín-García Patricia , Bautista José M , Diez Amalia , Puyet Antonio
Published: April 2011

Background

The anti-malarial activity of maslinic acid (MA), a natural triterpene which has been previously shown to exert a parasitostatic action on Plasmodium falciparum cultures, was analysed in vivo by using the Plasmodium yoelii 17XL murine model.

Methods

ICR mice were infected with P. yoelii and treated with a single dose of MA by a intraperitoneal injection of MA (40 mg kg-1 day-1) followed by identical dose administration for the following three days. Parasitaemia and accumulation of intraerythrocytic stages was monitored microscopically. To assess protective immunity, cured mice were challenged with the same dose of parasites 40 days after recovery from the primary infection and parasitaemia was further monitored for 30 days. Humoral response was tested by ELISA and visualization of specific anti-P. yoelii antibodies was performed by Western-blotting.

Results

ICR mice treated with MA increased the survival rate from 20% to 80%, showing an arrest of parasite maturation from day 3 to 7 after infection and leading to synchronization of the intraerythrocytic cycle and accumulation of schizonts by day 6, proving that MA also behaves as a parasitostatic agent in vivo. Mice which survived the primary infection displayed lower rates of parasitic growth, showing a decline of parasitaemia after day 15, and complete clearance at day 20. These mice remained immunoprotected, showing not malaria symptoms or detectable parasitaemia after rechallenge with the same lethal strain. The analysis of specific antibodies against P. yoelii, present in mice which survived the infection, showed a significant increase in the number and intensity of immunoreactive proteins, suggesting that the protected mice may trigger a strong humoral response.

Conclusion

The survival increase observed in MA-treated mice can be explained considering that the parasitostatic effect exerted by this compound during the first days of infection increases the chances to develop effective innate and/or acquired immune responses. MA may represent a new class of anti-malarial compounds which, as a consequence of its parasitostatic action, favours the development of more effective sterilizing immune responses.