bluuebunny/crossref_metadata_2025 huggingface dataset:
Reduction in thermal plasticity of life history traits in response to cold selection: an experimental evolution study using Drosophila melanogaster
Authors: Roy Rishav , Chattopadhyay Aradhya , Deb Sharma Sreebes , Mondal Aharna , Biswas Payel , Ghosh Shampa M.
Published: January 2025

Introduction

How selection influences phenotypic plasticity is an important question in evolutionary biology. We report an experimental evolution study that examined how prolonged selection at cold vs. warm temperature impacts the thermal plasticity of traits like reproductive output, body size, and body water content in Drosophila melanogaster.

Methods

We conducted the study on two sets of large, outbred fly populations: one maintained at the standard fly rearing temperature, i.e., 25°C, and another selected at cold temperature, i.e., 17°C, for 3.5 years. Both selection lines were derived from the same ancestral population.

Results and discussion

We found that while 25°C selected females lay significantly fewer eggs in cold compared to warm or optimal rearing temperature of 25°C, the 17°C selected females have consistent or canalized fecundity levels across warm and cold conditions. Sustained fecundity levels across cold and warm conditions are potential marks of adaptation to a broader thermal range. While phenotypic plasticity may aid in adaptation to new environments, for traits such as fecundity, consistent levels across environments, that is, low plasticity, may be more adaptive. We also found that male flies from cold vs. warm selection regimes differ in their thermal plasticity. Plasticity of dry weight and body water content was reduced in the cold-selected males, indicating the evolution of canalized levels for these traits too. While canalized fecundity levels across temperatures can potentially help in thermal adaptation, the significance of reduced plasticity of male body size and water content needs to be investigated in the future.
Intraspecific epigenomics divergence in brown bears (Ursus arctos): insights from genome-wide DNA methylation patterns
Authors: Colangelo Paolo , Bonapace Ian Marc , Gramolini Laura , Solano Emanuela , Desiato Elisa , Franchini Paolo , Gentile Leonardo , Guadagnini Roberto , Kleven Oddmund , Kindberg Jonas , Kopatz Alexander , Ciucci Paolo
Published: January 2025

Epigenetic mechanisms such as methylation can influence gene expression and play a crucial role in the adaptation to local environmental conditions, thereby introducing non-genetic variability within species. Here, using a Reduced Representation Bisulfite Sequencing approach (RRBS), we compared the methylation patterns in blood and muscle across three European brown bear populations. Our results clearly demonstrated that, beyond tissue-driven divergences, the methylation patterns of the three populations are significantly distinct. Differentially methylated sites, possibly associated with genomic features involved in development and anatomical differentiation, are widespread across the bear genome. This finding supports previous studies suggesting a role for the alteration of developmental pathways in shaping phenotypic novelties with potential adaptative significance. Our results underscore the importance and the effectiveness of including epigenetic approaches in studying wild non-model organisms. Investigating the epigenome can be especially relevant for endangered populations that have experienced a significant erosion of genomic diversity.
Bibliometric analysis of neutrophil extracellular traps induced by protozoan and helminth parasites (2008–2024)
Authors: Muñoz-Caro Tamara , Saraiva Elvira M. , Mariante Rafael M.
Published: January 2025

Introduction

Parasitic diseases pose a significant global public health challenge, affecting billions of people and causing substantial economic losses in livestock and poultry. In the fight against these infections, neutrophils play a crucial role, employing various strategies, including the release of neutrophil extracellular traps (NETs). Recent studies have made significant progress in understanding NETs triggered by protozoa and helminths. However, a comprehensive bibliometric analysis that compiles these findings and identifies research hotspots and trends in this field is still lacking.

Methods

We utilized the Web of Science Core Collection and Scopus databases to retrieve original articles on NETs induced by protozoa and helminths. After screening, the data was transferred to the visualization tools VOSviewer, Biblioshiny, and CiteSpace for analysis.

Results

Our study included 159 original articles published in 69 journals, involving 909 authors from 270 institutions across 41 countries. Germany and Brazil have made the most significant contributions to the research on NETs and parasites, accounting for 45 and 32 publications, and 1,495 and 1,342 citations, respectively. Carlos Hermosilla and Anja Taubert (Justus Liebig University Giessen, Germany), and Elvira Saraiva (Federal University of Rio de Janeiro, Brazil), are leaders in the field, both in terms of publication output and citations. Frontiers in Immunology has consistently and significantly impacted the field, and an article in the Proceedings of the National Academy of Sciences of the United States of America providing the first direct evidence of NETs release in response to a parasite is by far the most cited. Current research focuses on malaria, Toxoplasma gondii, Besnoitia besnoiti, nematodes, and the mechanisms of NETs production and their effects on parasites and host cells. Emerging trends include therapeutic targeting of NETs and comparative studies across different host and parasite species.

Conclusion

This study offers a comprehensive overview and visual analysis of NETs and parasites, highlighting key areas for future research.
Immune checkpoint inhibitor-induced diabetes mellitus: clinical characteristics and risk factors
Authors: Zhan Mei , Long Qinran , He Jinhan , Huang Litao , Wu Bin , Xu Haixia , Mo Li , Xu Ting
Published: January 2025

Background

Emerging evidence indicates that immune checkpoint inhibitor-induced diabetes mellitus (ICI-DM) might be more common than initially reported, and more different clinical pictures associated with ICI-DM were described.

Objective

The aim of our study was to identify the clinical characteristics and possible predictive factors of ICI-DM.

Methods

We conducted a retrospective review of patients who received immune checkpoint inhibitors (ICI) at West China Hospital, Sichuan University until June 2023. Patients were reviewed at death or on 7 May 2024. We applied logistic regression to study the associations between clinical characteristics and ICI-DM.

Results

Our study included 8,199 participants who received ICI between October 2014 and June 2023. Among them, 1,077 patients (13.14%) developed ICI-DM according to diagnostic criteria based on guidelines. By excluding patients influenced by glucocorticoids or immunosuppressants, ICI-DM was observed in 713 of 8,199 (8.70%) patients. In all patients, hypertension, hyperlipidemia, using glucocorticoids or immunosuppressants, lung cancer, and using more than one pathway of ICI were associated with a higher risk of ICI-DM. However, the risk factors for ICI-DM in patients without the influence of glucocorticoids or immunosuppressants were only hypertension, hyperlipidemia, and pancreatic lesions. In all patients and those patients without the influence of glucocorticoids and immunosuppressants, hypertension and hyperlipidemia may increase the risk for ICI-DM.

Conclusions

This large, real-world cohort demonstrates that the incidence of ICI-DM may be underestimated in previous literature. Blood glucose monitoring is needed in patients receiving ICI therapy.

Clinical trial registration

https://www.chictr.org.cn, identifier ChiCTR2300075974.
The association of systemic immune-inflammation index with incident breast cancer and all-cause mortality: evidence from a large population-based study
Authors: Li Yu , Yu Meng , Yang Ming , Yang Jingqi
Published: January 2025

Background

Chronic low-grade inflammation is recognized as a significant factor in various health outcomes, including the development and progression of breast cancer. The Systemic Immune-Inflammation Index (SII), a novel marker derived from routine blood counts, has been suggested as a predictor of all-cause mortality and cardiovascular mortality. However, its predictive value in a nationwide representative population, particularly for breast cancer incidence and mortality, is not well-established.

Methods

This study aimed to assess the association of SII and the risk of breast cancer incidence and all-cause mortality in breast cancer patients within the National Health and Nutrition Examination Survey (NHANES) from 1999 to 2018. SII was calculated from complete blood count parameters. We used multifactor regression models to examine the associations between SII and the outcomes of interest.

Results

A total of 21,058 female participants were included in the study, of which 557 (2.7%) were identified as having breast cancer. After adjusting for multiple potential confounders, the relationship between SII and the incidence of breast cancer revealed an inverse L-shaped association. The optimal inflection point for SII/100 was determined to be 5.09. Below this threshold, there was a significant increase in the risk of breast cancer (OR=1.05, 95% CI: 1.02-1.09). Within the breast cancer population, SII exhibited a J-shaped relationship with all-cause mortality. The optimal inflection point for SII/100 in this context was 5.22, and above this threshold, there was a marked escalation in all-cause mortality (HR=1.09, 95% CI: 1.04-1.14).

Conclusion

The SII, as a novel inflammatory composite index, is significantly associated with the risk of breast cancer incidence and all-cause mortality in breast cancer patients. These findings highlight the importance of monitoring systemic inflammation and suggest that SII could serve as a valuable prognostic tool.
IL-33/ST2 axis in diverse diseases: regulatory mechanisms and therapeutic potential
Authors: Sheng Feiya , Li Mi , Yu Jia-Mei , Yang Si-Yu , Zou Liang , Yang Guan-Jun , Zhang Le-Le
Published: January 2025

Interleukin-33 (IL-33) is a nuclear factor and member of the IL-1 cytokine family. IL-33 is mainly expressed by epithelial and endothelial cells and exerts its function through interaction with various immune cells, and binding to its receptor can form the IL-33/Suppression of tumorigenicity 2 (ST2) signaling pathway. While most cytokines are actively synthesized within cells, IL-33 is produced passively in response to tissue damage or cell necrosis, indicating its role as a signaling molecule following cellular infection, stress, or trauma. IL-33/ST2 signaling pathway has been proved to play diverse role in the pathological process of central nervous system disorders, cancer, fibrosis, autoimmune diseases, etc. Although research on the IL-33/ST2 signaling pathway has deepened recently, relevant treatment strategies have been proposed, and even targeted drugs are in the preclinical stage; further research on the effect of the IL-33/ST2 signaling pathway in different diseases is still necessary, to provide a clearer understanding of the different roles of IL-33/ST2 in disease progression and to develop new drugs and treatment strategies. Because IL-33/ST2 plays an important role in the occurrence and progression of diseases, the study of therapeutic drugs targeting this pathway is also necessary. This review focused on recent studies on the positive or negative role of IL-33/ST2 in different diseases, as well as the current related drugs targeting IL-33/ST2 in the preclinical and clinical stage. The mechanism of IL-33/ST2 in different diseases and its mediating effect on different immune cells have been summarized, as well as the antibody drugs targeting IL-33 or ST2, natural compounds with a mediating effect, and small molecule substances targeting relative pathway. We aim to provide new ideas and treatment strategies for IL-33/ST2-related drugs to treat different diseases.
Mechanisms and environmental factors shaping the ecosystem of brain macrophages
Authors: Penati Silvia , Brioschi Simone , Cai Zhangying , Han Claudia Z. , Colonna Marco
Published: January 2025

Brain macrophages encompass two major populations: microglia in the parenchyma and border-associated macrophages (BAMs) in the extra-parenchymal compartments. These cells play crucial roles in maintaining brain homeostasis and immune surveillance. Microglia and BAMs are phenotypically and epigenetically distinct and exhibit highly specialized functions tailored to their environmental niches. Intriguingly, recent studies have shown that both microglia and BAMs originate from the same myeloid progenitor during yolk sac hematopoiesis, but their developmental fates diverge within the brain. Several works have partially unveiled the mechanisms orchestrating the development of microglia and BAMs in both mice and humans; however, many questions remain unanswered. Defining the molecular underpinnings controlling the transcriptional and epigenetic programs of microglia and BAMs is one of the upcoming challenges for the field. In this review, we outline current knowledge on ontogeny, phenotypic diversity, and the factors shaping the ecosystem of brain macrophages. We discuss insights garnered from human studies, highlighting similarities and differences compared to mice. Lastly, we address current research gaps and potential future directions in the field. Understanding how brain macrophages communicate with their local environment and how the tissue instructs their developmental trajectories and functional features is essential to fully comprehend brain physiology in homeostasis and disease.
Abundance, occurrence, and degradation of airborne antibiotic resistance genes in coastal and marine atmospheres
Authors: Jia Shijie , Yao Xiaohong , Qi Jianhua , Liu Xiaohuan , Gao Huiwang
Published: January 2025

Antibiotic resistance genes (ARGs) may have significant impacts on human health and ecosystems. Airborne ARGs are reportedly widely distributed across inland cities, but little is known about their abundance in marine atmospheres. Here, we report observations of ambient ARGs during a cruise over the marginal seas of the Bohai Sea and Yellow Sea and compare them with ARGs in the coastal atmosphere. We characterized the ARGs in terms of their abundance, occurrence, degradation, and risk in the marine and coastal atmospheres. Using Na+ and Ca2+ as indicators of marine and continental aerosol sources, respectively, we quantified the mutual transport of airborne ARGs. Our results revealed that the airborne ARG abundances and the number of ARG types increased concomitantly with the mass concentrations of particulate matter because of the accumulation effect, but the ratios of ARG abundance/particulate matter concentration gradually decreased. The inconsistent trend suggested that the reduction in airborne ARGs was greater than their increase with bacterial reproduction during their accumulation and transport in the atmosphere. In addition, the number of ARG types in marine aerosols was greater than that in coastal aerosols. However, the airborne ARG abundance in marine aerosols was greater than that in clean coastal aerosols but not in polluted coastal aerosols. Some ARG types detected in marine aerosols were significantly and positively correlated with wind speed and relative humidity, implying that they may be derived from marine emissions, whereas the other ARGs are likely derived from long-range continental transport. Sea-derived airborne ARGs serve as important sources in coastal aerosols, but their contributions decrease with increasing air pollution levels. Our findings highlight the complex role of marine aerosols as both potential sources and reservoirs of airborne ARGs and highlight the critical importance of investigating the transport dynamics and variation mechanism during the long-range transport of ARGs.
Efficacy and safety of Xiongzhitongluo granules in the treatment of acute ischemic stroke: study protocol for a randomized controlled trial
Authors: Chen Yunmeng , Wei Jingjing , Liang Xiao , Liu Yue , Miao Lina , Zhao Di , Zhang Yunfan , Liu Hongxi , Zhang Yunling
Published: January 2025

Introduction

Acute ischemic stroke (AIS) poses a significant risk to human health. Intravenous thrombolysis and mechanical thrombectomy are essential treatments for AIS, offering substantial benefits for neurological recovery and brain protection. However, their efficacy is often limited by stringent time constraints and contraindications, restricting accessibility for certain patient populations. Investigating novel therapeutic strategies is, therefore, crucial. Our team developed Xiongzhitongluo granules specifically for AIS and is conducting a randomized controlled trial (RCT) to validate their effectiveness.

Methods and analysis

This multi-center, randomized, double-blind, placebo-controlled clinical trial includes 120 participants randomly allocated to the intervention or placebo group. Participants will receive a 14-day treatment alongside routine medications and will be monitored at multiple time points: days 1, 3, 5, 7, 14, 30, 60, and 90. The primary outcome is the change in the National Institutes of Health Stroke Scale (NIHSS) score from baseline to day 14. Secondary outcomes include the Scandinavian Stroke Scale (SSS), Barthel Index (BI), modified Rankin Scale (mRS), Brief Mini-Mental State Examination (MMSE), and traditional Chinese medicine (TCM) symptom assessment. Safety evaluations will include vital signs and laboratory tests. Data will be recorded using Epidata V3.1 and analyzed with SPSS 26.0.

Ethics and dissemination

This study received approval from the Ethics Committee of Xiyuan Hospital, China Academy of Chinese Medical Sciences (2021XLA102-2). Written informed consent was obtained from all participants.

Clinical trial registration

https://clinicaltrials.gov/, identifier, ChiCTR2200061859.
Advances, challenges and future applications of liver organoids in experimental regenerative medicine
Authors: Gong Da , Mo Jiaye , Zhai Mei , Zhou Fulin , Wang Guocai , Ma Shaohua , Dai Xiaoyong , Deng Xuesong
Published: January 2025

The liver is a vital organ responsible for numerous metabolic processes in the human body, including the metabolism of drugs and nutrients. After liver damage, the organ can rapidly return to its original size if the causative factor is promptly eliminated. However, when the harmful stimulus persists, the liver’s regenerative capacity becomes compromised. Substantial theoretical feasibility has been demonstrated at the levels of gene expression, molecular interactions, and intercellular dynamics, complemented by numerous successful animal studies. However, a robust model and carrier that closely resemble human physiology are still lacking for translating these theories into practice. The potential for liver regeneration has been a central focus of ongoing research. Over the past decade, the advent of organoid technology has provided improved models and materials for advancing research efforts. Liver organoid technology represents a novel in vitro culture system. After several years of refinement, human liver organoids can now accurately replicate the liver’s morphological structure, nutrient and drug metabolism, gene expression, and secretory functions, providing a robust model for liver disease research. Regenerative medicine aims to replicate human organ or tissue functions to repair or replace damaged tissues, restore their structure or function, or stimulate the regeneration of tissues or organs within the body. Liver organoids possess the same structure and function as liver tissue, offering the potential to serve as a viable replacement for the liver, aligning with the goals of regenerative medicine. This review examines the role of liver organoids in regenerative medicine.
Case report: The Montgomery T tube may be the preferred transition option for achieving a smooth extubation after tracheotomy when complicating airway pathology is present
Authors: Wang Jieqiong , Li Xun , Xu Weihua , Jiang Nenghui , Yang Bo , Chen Ming
Published: January 2025

Prolonged retention of tracheostomy tubes post-procedure often leads to complications, including granulation tissue overgrowth, airway narrowing, and laryngeal edema, necessitating delayed removal of the tracheostomy tube. Currently, a definitive therapeutic regimen capable of simultaneously resolving these complications and expediting tracheostomy decannulation remains elusive. Herein, we present an efficacious strategy addressing these airway morbidities and facilitating rapid tube removal. A 44-year-old male patient, who had undergone tracheostomy due to underlying disease, demonstrated substantial recovery following rehabilitation and was poised for tracheostomy tube extraction. However, bronchoscopic examination revealed severe granulation tissue at the stoma site and laryngeal edema, posing challenges to immediate decannulation. To tackle these issues concurrently while aiming for swift tube removal, we performed bronchoscopic intervention for granulation tissue excision, subsequently replacing the conventional tracheostomy tube with a Montgomery T tube as a transitional measure to restore normal ventilation. With additional rehabilitation fostering respiratory function enhancement, follow-up bronchoscopies confirmed no recurrence of granulations and significant reduction in laryngeal edema, thereby enabling the successful removal of the Montgomery T tube 2 months later, restoring the patient’s unassisted respiratory capacity. This case underscores a clinically pertinent insight: following resolution of local airway abnormalities impeding tracheostomy decannulation, the strategic implementation of a Montgomery T tube as a transitional phase merits serious consideration among clinicians managing patients with long-term tracheostomies. Our findings contribute to the development of more streamlined approaches to overcoming complexities associated with tracheostomy tube removal in clinical practice.
Plasma EphA2 level is a superior biomarker to Del-1 for sepsis diagnosis and prognosis
Authors: Lee Eun Hye , Shin Mi Hwa , Kwak Se Hyun , Choi Ji Soo , Leem Ah Young , Lee Su Hwan , Chung Kyung Soo , Kim Young Sam , Lee Sang-Guk , Park Moo Suk
Published: January 2025

Background

Sepsis, characterized by a dysregulated host response to infection, often leads to organ dysfunction, and vascular endothelial dysfunction plays a central role. The erythropoietin-producing hepatocellular carcinoma (Eph)A2 receptor is associated with increased vascular permeability; however, the developmental endothelial locus-1 (Del-1), has contrasting effects on endothelial function. Hence, we examined their potential as biomarkers of sepsis.

Methods

In total, 117 participants, including 20 healthy controls, 21 patients with systemic inflammatory response syndrome (SIRS), and 76 patients with sepsis, were enrolled in this study. Sepsis severity was assessed using the Acute Physiology and Chronic Health Evaluation (APACHE) II and the Sequential Organ Failure Assessment (SOFA) scores.

Results

The Median plasma EphA2 levels increased progressively from healthy controls to SIRS and sepsis cases (154.29, 293.52, and 554.24 pg/mL; all p < 0.05). The median plasma Del-1 levels were highest in healthy controls, lowest in SIRS, and intermediate level in sepsis (101.27, 16.88, and 36.9 pg/mL; all p < 0.001). The levels of both biomarkers were higher in 28-day non-survivors than in survivors, in patients with sepsis (EphA2:898.09 vs. 475.88 pg/mL, p < 0.001; Del-1:46.09 vs. 32.68 pg/mL, p = 0.193); however, only EphA2 was statistically significant. The area under the curve for the EphA2 was 0.74 in the receiver operating characteristic curve analysis for predicting 28-day mortality, whereas APACHE II, SOFA, and Del-1 showed values of 0.762, 0.614, and 0.595, respectively. Kaplan–Meier analysis using these cutoffs revealed that survival was significantly higher in the group with both low EphA2 and Del-1 levels compared to the group with high levels of both markers (p < 0.001).

Conclusion

Plasma EphA2 levels consistently increased with sepsis severity, suggesting its biomarker value for sepsis diagnosis and prognosis. In contrast, plasma Del-1 response was variable, indicating its limited prognostic utility.
Association of arm circumference with the prevalence of gallstones in United States adults: a retrospective analysis on US National Health and Nutrition Examination Survey
Authors: Wang Jianjun , Chen Xi , He Wei , Zeng Xintao , Yang Pei , Gong Jianping , Wang Decai
Published: January 2025

Background

Arm circumference (AC) is a measure of nutritional status and an indicator of the risk of developing diseases, such as metabolic disorders. However, its relationship with the prevalence of gallstone disease (GS), a metabolic disorder, is unknown. Consequently, this research sought to investigate the relationship between AC and the prevalence of GS among the general adult population in America.

Methods

Participant data were extracted from the National Health and Nutrition Examination Survey (NHANES) 2017–2020 cycle. GS was defined based on self-reported medical history. AC was measured following standardized protocols as the primary exposure variable. Multivariable logistic regression models were employed to assess the association between AC and GS. Dose–response relationships were evaluated using generalized additive models with smoothed curve fitting, and subgroup analyses were conducted to explore effect modification by key covariates such as age, sex, race, hypertension, diabetes, and body mass index.

Results

Overall, a total of 8,081 participants were included in this study, with 849 reporting a history of GS. After accounting for potential confounders, we discovered that each centimeter increase in AC was linked to an 8% rise in the prevalence of GS (Odd ratio = 1.08, 95% confidence interval: 1.07–1.10). Dose–response curves demonstrated a positive linear relationship between AC and the prevalence of GS, which, according to the results of the subgroup analyses, was consistent in the vast majority of subgroups, although there were subtle differences.

Conclusion

AC exhibited a linear and positive association with the prevalence of GS. Although a causal relationship between AC and the prevalence of GS could not be established, our study provides strong new support for the potential role of AC in the health assessments of adult populations.
Prevalence and pathogenic potential of Shewanella species in oysters and seawater collected from the Chesapeake Bay and Maryland Coastal Bays
Authors: Johnson Tahirah , Richards Gary P. , Jacobs John , Townsend Howard , Almuhaideb Esam , Rosales Detbra , Chigbu Paulinus , Dasilva Ligia , Parveen Salina
Published: January 2025

Shewanella is a genus of Gram-negative marine bacteria with some species associated with human and shellfish illnesses. This study evaluated the abundance of Shewanella species in oysters and seawater from the Chesapeake and Maryland Coastal Bays at four sites between 2019 and 2021. Physicochemical parameters such as temperature, salinity, dissolved oxygen, turbidity, pH, chlorophyll-a, rainfall within the last 48 h, total dissolved solids, and atmospheric pressure were also recorded to evaluate if there was a correlation between environmental parameters and the level of Shewanella. The highest total Shewanella counts were 1.8 × 107 CFU/g in oysters and 4.0 × 102 CFU/mL in seawater. 16S rRNA sequencing was performed on 1,344 representative isolates of which 890 (713 oyster, 177 seawater) were confirmed as Shewanella within 16 species. The top four species isolated from oysters and seawater were S. khirikhana a known shrimp pathogen (49%), S. marisflavi (19%), S. loihica (11%), and S. algae (8%). Testing for alpha and beta hemolysis were performed on all confirmed Shewanella isolates. Beta hemolysis was observed in 405 (46%) of the isolates of which 313 were in oysters and 92 in seawater. In oysters, beta-hemolysis was most prevalent in S. khirikhana (233 of 344 isolates, 68%), while in seawater 64 of 92 isolates (70%) were beta-hemolytic strains. Beta-hemolysis suggests that these could be potentially pathogenic strains. Correlations were performed between physicochemical attributes of the seawater and Shewanella counts. Only seawater temperature and dissolved oxygen correlated with Shewanella counts (r = 0.45 and − 0.41), respectively. No correlations were observed between the physicochemical parameters and Shewanella abundances in oysters. Results suggest that virulent strains of Shewanella may be present in oysters and seawater from the Chesapeake and Maryland Coastal Bays, perhaps as a consequence of rising seawater temperatures.
Mapping hippocampal glutamate in healthy aging with in vivo glutamate-weighted CEST (GluCEST) imaging
Authors: Pecsok Maggie K. , Robinson Heather , Atkins Ally , Calkins Monica E. , Elliott Mark A. , Mordy Arianna , Stifelman Jacquelyn , Gur Ruben C. , Moberg Paul J. , Nanga Ravi Prakash Reddy , Ruparel Kosha , Shinohara Russell T. , Wolk David A. , Reddy Ravinder , Roalf David R.
Published: January 2025

Introduction

Hippocampal glutamate (Glu) dysfunction is a pertinent indicator of neurodegeneration, yet mapping typical age-related changes in Glu has been challenging. Here, we use a 7T MRI approach, Glutamate Chemical Exchange Saturation Transfer (GluCEST), to measure bilateral hippocampal Glu in healthy old (HOA) and young (HYA) adults.

Methods

Bilateral hippocampal GluCEST data was acquired from 27 HOA and 22 HYA using 7T MRI. GluCEST differences by age and hemisphere were tested with a linear mixed model. GluCEST asymmetry index was also evaluated by age. Exploratory analyses examined associations between hippocampal GluCEST, age group, and scores on the Montreal Cognitive Assessment (MoCA) and Cognitive Complaints Index (CCI).

Results

GluCEST levels showed an age group and hemisphere interaction. In HOA, GluCEST was higher in left than right hippocampus, but in HYA, GluCEST level was equivalent across hemispheres. HOA had lower GluCEST than HYA in the right hippocampus. GluCEST asymmetry index confirmed significant left asymmetry in HOA. Lower GluCEST levels in HOA were associated with subjective cognitive complaints as measured by the CCI.

Discussion

Hippocampal GluCEST provides insight into age-related neural changes, with lower GluCEST in the right hippocampus in older adults. These findings offer a step toward elucidating the asymmetrical trajectory of hippocampal glutamatergic alterations and their relationship to cognitive phenotypes.
Associations between social networks, messaging apps, addictive behaviors, and sleep problems in adolescents: the EHDLA study
Authors: Navalón-González María , Montenegro-Espinosa José Adrián , Gutiérrez-Espinoza Héctor , Olivares-Arancibia Jorge , Yañéz-Sepúlveda Rodrigo , Duclos-Bastías Daniel , Garrido-Miguel Miriam , Mesas Arthur Eumann , López-Gil José Francisco , Jiménez-López Estela
Published: January 2025

Objective

The current study aims to provide a comprehensive analysis of the relationships between social network (SN) use, messaging apps use, and addictive behaviors related to SNs, and sleep-related problems in a sample of Spanish adolescents.

Methods

This was a cross-sectional study using data from the Eating Healthy and Daily Life Activities (EHDLA) project, which involved adolescents aged 12–17 years from three secondary schools in Valle de Ricote (Region of Murcia, Spain). A sample of 632 adolescents was studied. The use of SN (i.e., Facebook, Twitter, Instagram, Snapchat or TikTok) or messaging applications (i.e., WhatsApp) was assessed via a scale including one item for each SN, in which adolescents were asked what type of SN they used and the usage profile of each SN. The Short Social Networks Addiction Scale-6 Symptoms (SNAddS-6S) was used to determine SN addictive behaviors. Generalized linear regression analyses with a negative binomial distribution were performed to determine the associations of SN use or SN addictive behaviors with sleep-related problems. These analyses were adjusted for age, sex, body mass index, socioeconomic level, physical activity, sedentary behavior, and adherence to the Mediterranean diet.

Results

Higher SN use was related to greater presence of sleep-related problems [prevalence ratio (PR) = 1.04; 95% confidence interval (CI) 1.01–1.07; p = 0.015]. Additionally, the higher the score on the addictive behaviors toward SN use scale was, the more sleep-related problems were identified (PR = 1.15; 95% Cl 1.09 to 1.21; p < 0.001). Specifically, only the use of Twitter was significantly associated with sleep-related problems (PR = 1.10; 95% Cl 1.01 to 1.21; p = 0.035). In terms of addictive behaviors related to SN use, mood modification, relapse, withdrawal, and conflict were significantly associated with sleep-related problems (mood modification: PR = 1.58; 95% CI 1.36 to 1.84; p < 0.001; relapse: PR = 1.24; 95% CI 1.07 to 1.43; p = 0.004; withdrawal: PR = 1.28; 95% CI 1.08 to 1.51; p = 0.004; conflict: PR = 1.19; 95% CI 1.01 to 1.39; p = 0.037).

Conclusion

Our results suggest a relationship between SN use, SN addictive behaviors, and sleep-related problems in adolescents. These cross-sectional results should be confirmed in longitudinal and intervention studies.
A nomogram to predict postoperative new-onset cerebral infarction after revascularization of moyamoya disease in adults and its validation: a retrospective study
Authors: Wang Zhen , Yu Jiacheng , Zhang Yu , Ruan Jiaping , Liu Xiaojie , Ma Sijia , Xie Jun , Wu Mimi , Bo Jinhua , Sun Yu’e
Published: January 2025

Background

The new-onset cerebral infarction is frequent after revascularization of moyamoya disease (MMD) in adults, serving as a major public health issue worldwide. The present study aims to construct a nomogram to predict postoperative new-onset cerebral infarction (POCI) after revascularization of adult MMD.

Materials and methods

Clinical data of 653 cases of adult MMD treated with revascularization were retrospectively analyzed. They were randomly divided into a training set (n = 457) and a validation set (n = 196) at a ratio of 7:3. Based on the risk factors of POCI after revascularization of adult MMD identified by logistic regression analysis and the corresponding regression coefficients, a nomogram was constructed. Its performance to predict POCI after revascularization of adult MMD was validated by calculating the area under the curve (AUC) and the decision curve analysis.

Results

Univariate and multivariate logistic regression analyses showed that preoperative cerebral infarction (OR 2.548, 95% CI 1.357–4.787; p = 0.004), posterior cerebral artery anomalies (OR 2.106, 95% CI 1.157–3.834; p = 0.015), post-transit arterial development (OR 2.983, 95% CI 1.336–6.661; p = 0.008), pre-anesthesia mean arterial pressure > 102.830 mmHg (OR 3.329, 95% CI 1.938–5.721; p < 0.001), total operating time > 212.500 min (OR 2.256, 95% CI 1.239–4.140; p = 0.008), preoperative fibrinogen level > 2.750 g/L (OR 1.852, 95% CI 1.072–3.200; p = 0.027), and mean corpuscular hemoglobin concentration (OR 1.021, 95% CI 1.001–1.040; p = 0.038) were independent risk factors of POCI after revascularization of adult MMD. The AUC was 0.772 (95% CI 0.714–0.772) in the training set, and 0.718 (95% CI 0.603–0.833) in the validation set.

Conclusion

Collectively, the newly established nomogram effectively and intuitively predicts the POCI after revascularization of adult MMD.

Clinical trial registration

www.chictr.org, identifier ChiCTR2400087946.
Fucoidan alleviated colitis aggravated by fiber deficiency through protecting the gut barrier, suppressing the MAPK/NF-κB pathway, and modulating gut microbiota and metabolites
Authors: Zheng Weiyun , Tang Shuangru , Ren Xiaomeng , Song Shuang , Ai Chunqing
Published: January 2025

Insufficient dietary fiber intake has become a global public health issue, affecting the development and management of various diseases, including intestinal diseases and obesity. This study showed that dietary fiber deficiency enhanced the susceptibility of mice to colitis, which could be attributed to the disruption of the gut barrier integrity, activation of the NF-κB pathway, and oxidative stress. Undaria pinnatifida fucoidan (UPF) alleviated colitis symptoms in mice that fed with a fiber deficient diet (FD), characterized by increased weight gain and reduced disease activity index, liver and spleen indexes, and histological score. The protective effect of UPF against FD-exacerbated colitis can be attributed to the alleviation of oxidative stress, the preservation of the gut barrier integrity, and inhibition of the MAPK/NF-κB pathway. UPF ameliorated the gut microbiota composition, leading to increased microbiota richness, as well as increased levels of Muribaculaceae, Lactobacillaceae, and Bifidobacterium and reduced levels of Proteobacteria, Bacteroidetes, and Bacteroides. Metabolomics analysis revealed that UPF improved the profile of microbiota metabolites, with increased levels of carnitine and taurine and decreased levels of tyrosine and deoxycholic acid. This study suggests that UPF has the potential to be developed as a novel prebiotic agent to enhance human health.
A cohort study of serum 25-hydroxyvitamin D levels and the risk of hyperlipidaemia in adults
Authors: Liu Zi-yue , Liu Sha , Yao Xue , Wang Chun-yang , Song Yunhao , Bi Yan-ming , Wang Jin-Xiu , Li Yang , Shi Ta-la , Mi Wei , Chen Caiyun , Hu Zhi-Yong
Published: January 2025

Objective

This study aims to investigate the potential association between serum 25(OH)D levels and the risk of hyperlipidemia in adults through a prospective cohort study conducted in Zhejiang Province.

Methods

Baseline surveys and follow-up studies were conducted to collect and analyze follow-up data over a three-year period. Vitamin D deficiency was defined as 25(OH)D < 20 ng/mL, insufficiency as 20–29 ng/mL, and sufficiency as 25(OH)D ≥ 30 ng/mL. Hyperlipidemia or dyslipidemia was defined as the presence of hypercholesterolemia, hypertriglyceridemia, or both. The relationship between demographic characteristics and the incidence of hyperlipidemia among the study participants was explored.

Results

A total of 1,210 participants were included in this study, with 43.80% being male. The mean age of the participants was 51.84 ± 14.37 years, and the average serum 25(OH)D level was 25.89 (21.50, 29.82) ng/mL. A significant difference in the proportion of vitamin D deficiency was observed between males and females (22.06% vs. 10.94%, p < 0.001). Vitamin D deficiency and insufficiency were prevalent among the middle-aged and elderly population (78.24%). Significant differences were found between the two groups in multiple sociodemographic variables, behavioral factors, and metabolic risk factors (p < 0.05). The incidence of hyperlipidemia among vitamin D-deficient individuals was 1.612 times higher than that among vitamin D-sufficient individuals (95% confidence interval [CI]: 1.228–2.116; p < 0.001). After fully adjusting for confounding factors, the multivariate-adjusted hazard ratio (HR) was 1.572 (95% CI: 1.187–2.08; p = 0.002), indicating a difference in the incidence of hyperlipidemia across different serum vitamin D levels.

Conclusion

This cohort study reveals a significant association between serum 25(OH)D levels and the incidence of hyperlipidemia. Additionally, lifestyle factors associated with vitamin D deficiency are also correlated with the incidence of hyperlipidemia. These findings provide further evidence for improving blood lipid profiles through adjustments in vitamin D intake or related lifestyle modifications.
Mature tertiary lymphoid structure associated CD103+ CD8+ Trm cells determined improved anti-tumor immune in breast cancer
Authors: Fang Qin , Chen Shuru , Chen Xiaoyue , Zou Wei , Chen Di , Huang Yukang , Wu Chucheng
Published: January 2025

Background

Although tertiary lymphoid structures (TLS) play crucial roles in the anti-tumor immune response and are associated with favorable prognoses in many solid tumors, the precise mechanisms by which TLSs enhance anti-tumor immunity remain poorly understood. The current study aimed to explore the relationship between the maturity of tertiary lymphoid structures and their key immune cells in combating breast cancer.

Patients and methods

In this study, we utilized immunofluorescence and H&E staining to detect tumor-resident memory T cells (Trm) and assess the maturity of TLS, analyzing their distribution and proportion in an annotated cohort of 95 breast cancer patients.

Results

The presence of tumor-associated TLSs was correlated with an improved prognosis in patients with breast cancer. The proportion of CD8+CD103+ resident memory T cells and natural killer (NK) cells within the TLSs was significantly higher than that in areas outside of these structures. Additionally, the proportions of CD103+ CD8+ Trm cells and NK cells were significantly increased with the gradual maturation of TLS. Furthermore, the secretion function of effector molecules by CD8+ CD103+ Trm cells and NK cells within TLSs was significantly enhanced, indicating a strong correlation between the effector function of CD103+ CD8+ Trm and NK cells and the maturity of TLSs.

Conclusion

Our study identifies potential additional prognostic information for the clinical prognosis of breast cancer patients, underscoring the prognostic significance of immune cells within TLS, with a particular focus on CD103+ CD8+ Trm cells and NK cells.
Digital pathology and artificial intelligence in renal cell carcinoma focusing on feature extraction: a literature review
Authors: Li Ming-Yue , Pan Yu , Lv Yang , Ma He , Sun Ping-Li , Gao Hong-Wen
Published: January 2025

The integrated application of artificial intelligence (AI) and digital pathology (DP) technology has opened new avenues for advancements in oncology and molecular pathology. Consequently, studies in renal cell carcinoma (RCC) have emerged, highlighting potential in histological subtype classification, molecular aberration identification, and outcome prediction by extracting high-throughput features. However, reviews of these studies are still rare. To address this gap, we conducted a thorough literature review on DP and AI applications in RCC through database searches. Notably, we found that AI models based on deep learning achieved area under the curve (AUC) of over 0.93 in subtype classification, 0.89-0.96 in grading of clear cell RCC, 0.70-0,89 in molecular prediction, and over 0.78 in survival prediction. This review finally discussed the current state of researches and potential future directions.
Mini review of plant products as food supplement against MSG-induced liver injury: antioxidant, oxidative stress and histological prospects
Authors: Kasmara Dwi Pratiwi , Abdullah Erlina , Harun Zaliha , Sari Fatmi Nirmala , Abd Rashid Norhashima , Teoh Seong Lin
Published: January 2025

Monosodium glutamate (MSG) is an odorless white solid crystalline derived from the amino acid glutamic acid. It is widely used as a flavor enhancer, but its excessive consumption has been associated with toxicity to various organs. In MSG-induced liver injury, few mechanisms have been identified, which started with the generation of reactive oxygen species that leads to oxidative stress which further causes liver injury. In response to this health concern, there is growing interest in various plant products such as plant extracts, flavonoids and phenolic compounds that were able to minimize oxidative stress, serum transaminases and scavenge free radicals in the liver after MSG administration. This review explores the potential of various plant products as dietary supplements to MSG-induced liver injury, focusing on their antioxidant activities, modulatory effects on liver function markers, and histological outcomes. By compiling this evidence, this review provides insights into their potential as preventive strategies against MSG-related liver toxicity, supporting their inclusion in dietary regimens for the maintenance of liver function.
Effect of a mindfulness-based cognitive behavior therapy intervention on occupational burnout among school teachers
Authors: Paudel Netra Raj , K. C. Prakash , Nygård Clas-Håkan , Neupane Subas
Published: January 2025

Background

We aimed to assess the effect of mindfulness-based cognitive behavior therapy (MB-CBT) as an intervention to reduce burnout among secondary school teachers in Nepal.

Methods

The baseline survey of this randomized controlled trial included 218 secondary school teachers from 37 schools in Kathmandu. At a one-month follow-up, 192 teachers completed post-test survey. The Maslach Burnout Inventory for Educators (MBI-ES) tool was used to measure burnout in three dimensions: emotional exhaustion (EE), depersonalization (DP), and personal accomplishment (PA). Teachers in the treatment arm (n=102) received MB-CBT, while those in the control arm (n=116) continued their regular activities. Linear regression models with generalized estimating equations were used to calculate the mean, mean change estimates, and their 95% confidence intervals (CIs).

Results

The mean level of EE decreased during the post-test for both treatment (mean change -0.93, 95% CI: -2.56 to 0.70) and control (-1.71, 95% CI: -3.24 to -0.18) arms. The mean level of DP also decreased in the treatment arm (-0.12, 95% CI: -0.98 to -0.75), but a sharper decline was observed in the control arm (-1.97, 95% CI: -2.78 to -1.16). The mean level of PA increased in both the treatment (1.04, 95% CI: -0.21 to 2.30) and control (1.53, 95% CI: 0.35 to 2.70) arms.

Conclusions

The prevalence of high EE and low PA decreased in both arms. However, the prevalence of high DP remained constant in the treatment arm but sharply decreased in the control arm. No significant differences between the arms were found in the post-test mean levels of EE, DP, and PA. The virtual MB-CBT intervention showed no significant impact on reducing burnout. Nonetheless, small reductions in burnout were observed in both groups.
Managing Water-Ecosystem-Food Nexus using participatory approaches: insights from an innovative methodological approach developed in two Mediterranean areas
Authors: Malamataris Dimitrios , Pisinaras Vassilios , Pagano Alessandro , Baratella Valentina , Vanino Silvia , Bea Manuel , Babakos Konstantinos , Chatzi Anna , Fabiani Stefano , Giordano Raffaele , Kafkias Petros , López-Moya Estrella , Papadaskalopoulou Christina , Portoghese Ivan , Tassopoulos Dimitris , Panagopoulos Andreas
Published: January 2025

The formulation of effective policies to address both the present allocation and future conservation of natural resources in an integrated way remains an essential and challenging task. In this regard, managing the Nexus is increasingly seen as a priority in resource governance. Nature-based Solutions (NbS) are gradually being advocated to enhance sustainable and resilient Nexus management efforts. Designing and planning NbS tailored to local contexts requires a thorough understanding of the specific challenges and perspectives, as well as the divergent perspectives among stakeholders. This paper presents and analyses an effective stakeholder engagement strategy, based on the Learning & Action Alliances (LAA) scheme and aimed at identifying Water-Ecosystems-Food (WEF) Nexus challenges and selecting NbS in two case studies in the Mediterranean area (Greece and Italy). In total, 60 stakeholders were engaged in more than 40 events (workshops, seminars, open days, field trips etc.), while 25 individual interviews were also conducted. By fostering collaboration and stakeholder ownership, the methodology provided actionable insights and promoted context-specific solutions. The stakeholders proposed 24 NbSs in the Italian case study, most of which were related to agricultural landscape management, while in the Greek case study, 2 of the 4 top-ranked measures were NbSs. The findings underline the importance of participatory approaches and transdisciplinary tools in addressing Nexus challenges, offering a replicable framework for sustainable resource management in resource-stressed regions.