bluuebunny/crossref_metadata_2025 huggingface dataset:
Numerical Investigation of Very-Large-Scale Motions in a Turbulent Boundary Layer for Different Roughness
Authors: Ren Hehe , Laima Shujin , Li Hui
Published: February 2020

Wall-model large eddy simulations (WMLES) are conducted to investigate the spatial features of large-scale and very-large-scale motions (LSMs and VLSMs) in turbulent boundary flow in different surface roughnesses at a very high Reynolds number, O (106–107). The results of the simulation of nearly smooth cases display good agreement with field observations and experimental data, both dimensioned using inner and outer variables. Using pre-multiplied spectral analysis, the size of VLSMs can be reduced or even disappear with increasing roughness, which indirectly supports the concept that the bottom-up mechanism is one of the origins of VLSMs. With increases in height, the power of pre-multiplied spectra at both high and low wavenumber regions decreases, which is consistent with most observational and experimental results. Furthermore, we find that the change in the spectrum scaling law from −1 to −5/3 is a gradual process. Due to the limitations of the computational domain and coarse grid that were adopted, some VLSMs and small-scale turbulence are truncated. However, the size of LSMs is fully accounted for. From the perspective of the spatial correlation of the flow field, the structural characteristics of VLSMs under various surface roughnesses, including three-dimensional length scales and inclination angles, are obtained intuitively, and the conclusions are found to be in good agreement with the velocity spectra. Finally, the generation, development and extinction of three-dimensional VLSMs are analyzed by instantaneous flow and vorticity field, and it shows that the instantaneous flow field gives evidence of low-speed streamwise-elongated flow structures with negative streamwise velocity fluctuation component, and which are flanked on each side by similarly high-speed streamwise-elongated flow structures. Moreover, each of the low-speed streamwise-elongated flow structure lies beneath many vortices.
Fuel Conservation for Launch Vehicles: Falcon Heavy Case Study
Authors: Jozič Primož , Zidanšek Aleksander , Repnik Robert
Published: February 2020

Space exploration has recently been growing at an increasing pace and has caused a significant burden to the environment, in particular, during the launch of rockets, when a large amount of fuel is burned and the exhaust gases are released in the air. For this case study, we selected the SpaceX Falcon Heavy reusable heavy-lift launch vehicle, which is one of the most promising rockets for the low-cost lifting of heavy payloads into orbit and beyond. We evaluated several strategies for optimisation of fuel consumption and for minimisation of environmental impact during launch through the atmosphere for the case of its first launch on February 6, 2018, when the rocket carried a red Tesla Roadster with a “Starman” in the direction toward Mars. In addition to the flight plan and Newtonian equations of motion, we have taken into account the thermodynamic properties of the rocket engines. Results are similar but slightly different if one minimises the total fuel consumption for the desired flight plan or if one minimises the environmental pollution during the initial stage of the launch through the atmosphere. The same methodology can be extended for launches in other directions including the Earth orbit and the Moon.
Scheduling Optimization of Time-Triggered Cyber-Physical Systems Based on Fuzzy-Controlled QPSO and SMT Solver
Authors: Jian Jie , Wang Lide , Chen Huang , Nie Xiaobo
Published: February 2020

The time-triggered communication paradigm is a cost-efficient way to meet the real-time requirements of cyber-physical systems. It is a non-deterministic polynomial NP-complete problem for multi-hop networks and non-strictly periodic traffic. A two-level scheduling approach is proposed to simplify the complexity during optimization. In the first level, a fuzzy-controlled quantum-behaved particle swarm optimization (FQPSO) algorithm is proposed to optimize the scheduling performance by assigning time-triggered frame instances to the basic periods of each link. In order to prevent population from high aggregation, a random mutation mechanism is used to disturb particles at the aggregation point and enhance the diversity at later stages. Fuzzy logic is introduced and well designed to realize a dynamic adaptive adjustment of the contraction–expansion coefficient and mutation rate in FQPSO. In the second level, we use an improved Satisfiability Modulo Theories (SMT) scheduling algorithm to solve the collision-free and temporal constraints. A schedulability ranking method is proposed to accelerate the computation of the SMT-based incremental scheduler. Our approach can co-optimize the jitter and load balance of communication for an off-line schedule. The experiments show that the proposed approach can improve the performance of the scheduling table, reduce the optimization time, and reserve space for incremental messages.
A Novel Servovalve Pilot Stage Actuated by a Piezo-electric Ring Bender: A Numerical and Experimental Analysis
Authors: Tamburrano Paolo , Plummer Andrew R. , De Palma Pietro , Distaso Elia , Amirante Riccardo
Published: February 2020

Electrohydraulic servovalves are widely used for precise motion control in aerospace and other industries due to their high accuracy and speed of response. However, commercial two-stage servovalves have several undesirable characteristics, such as the power consumption caused by the quiescent flow (internal leakage) in the pilot stage, and the complexity and high number of parts of the torque motor assembly, which affect the cost and the speed of manufacture. The solution to these problems can help to reduce costs, weight and power consumption, and enhance the reliability and reproducibility as well as the performance of these valves. For these reasons, this paper proposes a novel configuration for the pilot stage: it is composed of two normally closed two-way two-position (2/2) valves actuated by two piezo-electric ring benders; the opening and closing of the two piezo-valves can generate a differential pressure to be used to control the displacement of the main spool. In this way, there is negligible quiescent flow when the main stage is at rest; in addition, the torque motor and all its components are removed. To assess the performance of this novel pilot stage concept, a prototype of the piezo-valve has been constructed and tested. The experimental results indicate that the response speed of the new piezo-valve is very high. Furthermore, a numerical model is employed to show that, by adjusting specific parameters, the performance of the piezo-valve can be further improved, so that the valve can be fully opened or closed in less than 5 ms.
Coating and Functionalization Strategies for Nanogels and Nanoparticles for Selective Drug Delivery
Authors: Pinelli Filippo , Perale Giuseppe , Rossi Filippo
Published: February 2020

Drug delivery is a fascinating research field with several development opportunities. Great attention is now focused on colloidal systems, nanoparticles, and nanogels and on the possibility of modifying them in order to obtain precise targeted drug delivery systems. The aim of this review is to give an overview of the main available surface functionalization and coating strategies that can be adopted in order to modify the selectivity of the nanoparticles in the delivery process and obtain a final system with great targeted drug delivery ability. We also highlight the most important fields of application of these kinds of delivery systems and we propose a comparison between the advantages and disadvantages of the described functionalization strategies.
Gender and Musculoskeletal Comorbidity Impact on Physical Functioning in Elderly after Hip Fracture: The Role of Rehabilitation
Authors: Radosavljevic Katarina , Dragovic-Lukic Gordana , Nikolic Dejan , Radovic Pavle , Milicic Biljana , Radosavljevic Natasa
Published: February 2020

The study aim was to evaluate the effects of presence and level of musculoskeletal impairment along with gender on physical functioning outcome after the rehabilitation program in aged adults with a hip fracture. We analyzed 203 elderly people with hip fractures above 65 years of age that were treated after the hip surgery. According to the time of examination, patients were tested three times: at admission, discharge, and at three months post-discharge. Musculoskeletal impairments were analyzed, and for the estimation of severity of degree impairment, we used a cumulative index rating scale for geriatrics (CIRS-G). Regarding the gender, we separately analyzed males and females. To evaluate physical functioning of aged adults after a hip fracture, we used the physical functioning component (PFC) from the quality of life (SF-36) questionnaire. For males, on all three occasions we found non-significant differences were found in SF-36 PFC values between different degrees of CIRS-G musculoskeletal impairment. A significant difference was noticed in females three months post-discharge. Effects size of different examination periods for every CIRS-G severity degree of musculoskeletal impairment were high, where males had higher values for severity degrees 1 and 2, and females had higher values for severity degrees 0 and 3. Our findings might suggest that there is a certain degree of different rehabilitation treatment effects for males versus females. Moreover, it might be assumed that other factors could influence different degrees of functional improvement and outcome of individuals after a hip fracture with musculoskeletal impairment.
Effect of pH on Cucumber Growth and Nutrient Availability in a Decoupled Aquaponic System with Minimal Solids Removal
Authors: Blanchard Caroline , Wells Daniel , Pickens Jeremy , Blersch David
Published: February 2020

Decoupled aquaponic systems are gaining popularity as a way to manage water quality in aquaponic systems to suit plant and fish growth independently. Aquaponic systems are known to be deficient in several plant-essential elements, which can be affected by solution pH to either increase or decrease available nutrients. To determine the effect of pH in a decoupled aquaponic system, a study was conducted using aquaculture effluent from tilapia culture tanks at four pH treatments: 5.0, 5.8, 6.5, and 7.0, used to irrigate a cucumber crop. Growth and yield parameters, nutrient content of the irrigation water, and nutrients incorporated into the plant tissue were collected over two growing seasons. pH did not have a practical effect on growth rate, internode length or yield over the two growing seasons. Availability and uptake of several nutrients were affected by pH, but there was no overarching effect that would necessitate its use in commercial systems. Nutrient concentrations in the aquaculture effluent would be considered low compared to hydroponic solutions; however, elemental analysis of leaf tissues was within the recommended ranges. Research into other nutrient sources provided by the system (i.e., solid particles carried with the irrigation water) would provide further information into the nutrient dynamics of this system.
Stem Cell-Derived Exosomes in Autism Spectrum Disorder
Authors: Alessio Nicola , Brigida Anna Lisa , Peluso Gianfranco , Antonucci Nicola , Galderisi Umberto , Siniscalco Dario
Published: February 2020

Neurodevelopmental lifelong pathologies defined by problems with social interaction, communication capacity and presence of repetitive/stereotyped clusters of behavior and interests are grouped under the definition of autism spectrum disorder (ASD). ASD prevalence is still increasing, indicating the need to identify specific biomarkers and novel pharmacotherapies. Neuroinflammation and neuro-immune cross-talk dysregulation are specific hallmarks of ASD, offering the possibility of treating these disorders by stem cell therapy. Indeed, cellular strategies have been postulated, proposed and applied to ASD. However, less is known about the molecular action mechanisms of stem cells. As a possibility, the positive and restorative effects mediated by stem cells could be due to their paracrine activity, by which stem cells produce and release several ameliorative and anti-inflammatory molecules. Among the secreted complex tools, exosomes are sub-organelles, enriched by RNA and proteins, that provide cell-to-cell communication. Exosomes could be the mediators of many stem cell-associated therapeutic activities. This review article describes the potential role of exosomes in alleviating ASD symptoms.
An Overview on the Associations between Health Behaviors and Brain Health in Children and Adolescents with Special Reference to Diet Quality
Authors: Naveed Sehrish , Lakka Timo , Haapala Eero A.
Published: February 2020

Unhealthy diet has been associated with overweight, obesity, increased cardiometabolic risk, and recently, to impaired cognition and academic performance. The aim of this review is to provide an overview of the associations between health behaviors and cognition and academic achievement in children and adolescents under 18 years of age with a special reference to diet quality. Dietary patterns with a low consumption of fish, fruits, and vegetables, and high in fast food, sausages, and soft drinks have been linked to poor cognition and academic achievement. The studies on the associations between the high intake of saturated fat and red meat and low intake of fiber and high-fiber grain products with cognition are limited. The available evidence and physiological mechanisms suggest that diet may have direct, indirect, and synergistic effects on brain and cognition with physical activity, sedentary behaviors, cardiometabolic health, and sleep, but the associations have been modest. Therefore, integrating a healthy diet, physically active lifestyle, and adequate sleep may provide optimal circumstances for brain development and learning. We conclude that most of the existing literature is contained in cross-sectional studies, which therefore highlights the need for longitudinal and intervention studies on the effects of diet, physical activity, sedentary behavior, and sleep on cognition and academic performance.
Characteristics of Polycyclic Aromatic Hydrocarbons (PAHs) and Common Air Pollutants at Wajima, a Remote Background Site in Japan
Authors: Zhang Xuan , Zhang Lulu , Yang Lu , Zhou Quanyu , Xing Wanli , Toriba Akira , Hayakawa Kazuichi , Wei Yongjie , Tang Ning
Published: February 2020

Background: Background sites are mainly affected by long-range-transported air pollutants, resulting in potential adverse effects on local atmospheric environments. A 4–5 year observational study was conducted to illustrate the air pollution profile at the Kanazawa University Wajima air monitoring station (KUWAMS), an ideal remote background site in Japan. Methods: Nine polycyclic aromatic hydrocarbons (PAHs) in the particulate phase and various air pollutants were continuously monitored for 4–5 years. Diagnostic ratios of PAHs and back-trajectory analysis were applied to trace the possible sources of the air pollutants collected at the sampling site. Results: The atmospheric concentration of PAHs in the atmosphere at the site decreased from 2014 to 2019, benefit from the predominant air pollution control policy in China and Japan. Common air pollutants including sulfur dioxide (SO2), nitrogen oxides (NOx), ozone, methane (CH4), and non-methane hydrocarbon (NMHC) were detected in low concentrations from 2016 to 2019, while ozone (O3) and particulate matter (PM2.5, PM with a diameter less than 2.5 μm) were present in high levels that exceeded the Japanese standards. Most air pollutants peaked in spring and showed evident diurnal variations in spring and summer. Conclusions: This is the first study to clarify the atmospheric behaviors of multiple air pollutants at a background site in Japan. Significant external air pollutant impact and unneglectable air pollution were demonstrated at KUWAMS, indicating the importance of studying atmospheric pollution at remote sites.
Influences of Age, Sex and Smoking Habit on Flavor Recognition in Healthy Population
Authors: Nettore Immacolata Cristina , Maione Luigi , Desiderio Silvio , De Nisco Emma , Franchini Fabiana , Palatucci Giuseppe , Ungaro Paola , Cantone Elena , Macchia Paolo Emidio , Colao Annamaria
Published: February 2020

(1) Background: Flavor is one of the main factors influencing food preferences and dietary choices, and a reduction in flavor recognition has been associated with several diseases. A novel quantitative test to assess flavor has been recently developed and validated. The aim of the present work was to define the standard of flavor recognition in the general healthy population. (2) Methods: Three hundred and forty-eight healthy volunteers (18–80 years) performed the flavor test (FT). The test consisted of the oral administration of aqueous aromatic solutions, identifying 21 different compounds. Flavor score (FS) was calculated as the sum of the properly recognized flavors (range 0–21). (3) Results: Normal ranges for FT were produced. Flavor recognition was found to decrease with age. Females obtained slightly higher scores than males, mostly at older ages. Cigarette smoking seemed not to influence flavor recognition. (4) Conclusion: The normal values found for the flavor test in the healthy population will allow its usage as a diagnostic tool in several diseases.
Prostate Cancer Incidence in U.S. Counties and Low Levels of Arsenic in Drinking Water
Authors: Ahn Jaeil , Boroje Isabella J. , Ferdosi Hamid , Kramer Zachary J. , Lamm Steven H.
Published: February 2020

Background: Although inorganic arsenic in drinking water at high levels (100s–1000s μg/L [ppb]) increases cancer risk (skin, bladder, lung, and possibly prostate), the evidence at lower levels is limited. Methods: We conducted an ecologic analysis of the dose-response relationship between prostate cancer incidence and low arsenic levels in drinking water in a large study of U.S. counties (N = 710). County arsenic levels were <200 ug/L with median <100 ug/L and dependency greater than 10%. Groundwater well usage, water arsenic levels, prostate cancer incidence rates (2009–2013), and co-variate data were obtained from various U.S. governmental agencies. Poisson and negative-binomial regression analyses and stratified analysis were performed. Results: The best fitting polynomial analysis yielded a J-shaped linear-quadratic model. Linear and quadratic terms were significant (p < 0.001) in the Poisson model, and the quadratic term was significant (p < 0.05) in the negative binomial model. This model indicated a decreasing risk of prostate cancer with increasing arsenic level in the low range and increasing risk above. Conclusions: This study of prostate cancer incidence in US counties with low levels of arsenic in their well-water arsenic levels finds a j-shaped model with decreasing risk at very low levels and increasing risk at higher levels.
Grey System Theory in the Study of Medical Tourism Industry and Its Economic Impact
Authors: Dang Hoang-Sa , Nguyen Thuy-Mai-Trinh , Wang Chia-Nan , Day Jen-Der , Dang Thi Minh Han
Published: February 2020

The Asia-Pacific region is known as a favorite destination for global medical travelers due to its medical expertise, innovative technology, safety, attractive tourism destination and cost advantage in the recent decade. This study contributes to propose an approach which effectively assesses performance of medical tourism industry based on considering the economic impact factors as well as provides a conceptual framework for the industry analysis. Grey system theory is utilized as a major analyzing approach. According to that, factors impact on the sustainable development of medical tourism in Asia-Pacific region could be identified. The performance of each destination in this region was simultaneously revealed. The results presented an overall perspective of the medical tourism industry in the scope of the Asia-Pacific region, and in Taiwan particularly. Data was collected on six major destinations including Singapore, Thailand, India, South Korea, Malaysia and Taiwan. The results proved that tourism sources and healthcare medical infrastructures play a crucial role in promoting the healthcare travel industry, while cost advantage and marketing effectiveness were less considered. In addition, performance analyse indicated that Thailand has a good performance and stands in the top ranking, followed by Malaysia, India, Singapore, South Korea and Taiwan, respectively. The revenue of Taiwan has increased slowly in the last six years, with a market worth approximately NT$20.5 billion, and the number of medical travelers is expected to increase to 777,523 by 2025. The findings of this study are expected to provide useful information for the medical tourism industry and related key players in strategic planning.
Is Sensible Heat Flux Useful for the Assessment of Thermal Vulnerability in Seoul (Korea)?
Authors: Kwon You Jin , Lee Dong Kun , Kwon You Ha
Published: February 2020

Climate change has led to increases in global temperatures, raising concerns regarding the threat of lethal heat waves and deterioration of the thermal environment. In the present study, we adopted two methods for spatial modelling of the thermal environment based on sensible heat and temperature. A vulnerability map reflecting daytime temperature was derived to plot thermal vulnerability based on sensible heat and climate change exposure factors. The correlation (0.73) between spatial distribution of sensible heat vulnerability and mortality rate was significantly greater than that (0.30) between the spatial distribution of temperature vulnerability and mortality rate. These findings indicate that deriving thermally vulnerable areas based on sensible heat are more objective than thermally vulnerable areas based on existing temperatures. Our findings support the notion that the distribution of sensible heat vulnerability at the community level is useful for evaluating the thermal environment in specific neighbourhoods. Thus, our results may aid in establishing spatial planning standards to improve environmental sustainability in a metropolitan community.
Pesticide Use and Serum Acetylcholinesterase Levels among Flower Farm Workers in Ethiopia—A Cross-Sectional Study
Authors: Shentema Meaza Gezu , Kumie Abera , Bråtveit Magne , Deressa Wakgari , Ngowi Aiwerasia Vera , Moen Bente E.
Published: February 2020

The flower industry in East Africa has grown in recent years, especially in the production and export of roses. The aim of this study was to assess pesticide use on selected flower farms in Ethiopia. Serum cholinesterase levels in workers were used as a marker of pesticide exposure. This study was a cross-sectional study involving 588 workers from 15 different flower farms. It had a response rate of 95.5%. The participants included 277 males (mean age 26 years; 148 pesticide sprayers and 129 non-sprayers) and 311 females (mean age 25 years; 156 working in greenhouses and 155 working outside the greenhouses). The researchers undertook structured interviews, blood sampling, and walkthrough surveys. Descriptive statistics and Poisson regression were used in the statistical analyses. A total of 154 different trade names of pesticides were found. Of them, 31 (27%) were classified as moderately hazardous by the WHO, and 9% were organophosphates. Serum levels of cholinesterase deviating from 50–140 Michel units were considered abnormal. Abnormal serum cholinesterase levels (above 140 Michel units) were found in 97 participants (16.5%, 95% confidence interval 13.7–19.7%). There were no differences between the four job groups regarding cholinesterase levels. The high prevalence of abnormal serum cholinesterase levels might indicate the presence of pesticide intoxication. Thus, there is a need for routine monitoring of all workers exposed to pesticides, not only sprayers.
Influence of Contextual Variables in the Changes of Direction and Centripetal Force Generated during an Elite-Level Soccer Team Season
Authors: Granero-Gil Paulino , Bastida-Castillo Alejandro , Rojas-Valverde Daniel , Gómez-Carmona Carlos D. , de la Cruz Sánchez Ernesto , Pino-Ortega José
Published: February 2020

The study of the contextual variables that affect soccer performance is important to be able to reproduce the competition context during the training sessions. Therefore, the aim of the present study was to evaluate the effect of match outcome as related to goal difference (large win, >2 goals, LW; narrow win, 1–2 goals, NW; drawing, D; narrow loss, 1–2 goals, NL; or large loss, >2 goals, LL), match location (home, H; away, A; neutral, N), type of competition (international, INT; national, NAT; friendly, F), phase of the season (summer preseason, SPS; in-season 1, IS1; winter preseason, WPS; in-season 2), and the field surface (natural grass, NG; artificial turf, TF) on the change of direction (COD) and centripetal force (CentF) generated during official games. Thirty male elite-level soccer players (age: 26.57 ± 5.56 years) were assessed while using WIMU PROTM inertial devices (RealTrack Systems, Almeria, Spain) in 38 matches during the 2017–2018 season, selecting for analysis the number of COD at different intensities and the CentF, depending on the turn direction. Statistical analyses comprised a one-way ANOVA with the Bonferroni post-hoc and t-test for independent samples. The main results showed that the match outcome (ωp2 = 0.01–0.04; NW = D = NL > LL), match location (ωp2 = 0.01–0.06; A = N > H), type of competition (ωp2 = 0.01–0.02; INT > NAT > F), and period of the season (ωp2 = 0.01–0.02; SPS = IS1 = WPS > IS2) all exert some influence. No effect was found for the playing surface. Therefore, match outcome, match location, type of competition, and period of the season influence the demands of centripetal force and changes of direction. These aspects should be considered in the design of training sessions and microcycle workload planning during the season to improve competitive success.
Long-Term Exposure to Benzo[a]Pyrene Affects Sexual Differentiation and Embryos Toxicity in Three Generations of Marine Medaka (Oryzias Melastigma)
Authors: Sun Dong , Chen Qi , Zhu Bo , Lan Yu , Duan Shunshan
Published: February 2020

Benzo[a]pyrene (BaP) is a common environmental disrupting chemical that can cause endocrine disorders in organisms. However, the continued interference effects of BaP on multi-generation fish needs further research. In this study, we performed different periods (G1F1-3, G2F2-3, G3F3) of BaP exposure on marine medaka. We determined the embryo toxicity, and analyzed relative reproductive genes (ERα, cyp19a and vtg1) to predict the sexual differentiation of marine medaka. The results showed that high concentrations of BaP (200 μg·L−1) significantly delayed the hatching time of embryos. Moreover, medium/high concentrations of BaP (20 and 200 μg·L−1) prolonged the sexual maturity time of marine medaka. The relative gene expression of ERα, cyp19a and vtg1 were measured at 5 dpf of embryos. We found that BaP had significantly inhibited the expression of the genes related to female fish development. Consequently, there were more males in the offspring sex ratio at BaP exposure. Overall, BaP can cause embryonic toxicity and abnormal sexual differentiation, while the expression of related reproductive genes can effectively indicate the sex ratio.
Autism Spectrum Disorder Diagnostic System Using HOS Bispectrum with EEG Signals
Authors: Pham The-Hanh , Vicnesh Jahmunah , Wei Joel Koh En , Oh Shu Lih , Arunkumar N. , Abdulhay Enas. W. , Ciaccio Edward J. , Acharya U. Rajendra
Published: February 2020

Autistic individuals often have difficulties expressing or controlling emotions and have poor eye contact, among other symptoms. The prevalence of autism is increasing globally, posing a need to address this concern. Current diagnostic systems have particular limitations; hence, some individuals go undiagnosed or the diagnosis is delayed. In this study, an effective autism diagnostic system using electroencephalogram (EEG) signals, which are generated from electrical activity in the brain, was developed and characterized. The pre-processed signals were converted to two-dimensional images using the higher-order spectra (HOS) bispectrum. Nonlinear features were extracted thereafter, and then reduced using locality sensitivity discriminant analysis (LSDA). Significant features were selected from the condensed feature set using Student’s t-test, and were then input to different classifiers. The probabilistic neural network (PNN) classifier achieved the highest accuracy of 98.70% with just five features. Ten-fold cross-validation was employed to evaluate the performance of the classifier. It was shown that the developed system can be useful as a decision support tool to assist healthcare professionals in diagnosing autism.
Improving Screening Uptake among Breast Cancer Survivors and Their First-Degree Relatives at Elevated Risk to Breast Cancer: Results and Implications of a Randomized Study in the State of Georgia
Authors: Lipscomb Joseph , Escoffery Cam , Gillespie Theresa W. , Henley S. Jane , Smith Robert A. , Chociemski Toni , Almon Lyn , Jiang Renjian , Sheng Xi , Goodman Michael , Ward Kevin C.
Published: February 2020

Women diagnosed with breast cancer at a relatively early age (≤45 years) or with bilateral disease at any age are at elevated risk for additional breast cancer, as are their female first-degree relatives (FDRs). We report on a randomized trial to increase adherence to mammography screening guidelines among survivors and FDRs. From the Georgia Cancer Registry, breast cancer survivors diagnosed during 2000–2009 at six Georgia cancer centers underwent phone interviews about their breast cancer screening behaviors and their FDRs. Nonadherent survivors and FDRs meeting all inclusion criteria were randomized to high-intensity (evidence-based brochure, phone counseling, mailed reminders, and communications with primary care providers) or low-intensity interventions (brochure only). Three and 12-month follow-up questionnaires were completed. Data analyses used standard statistical approaches. Among 1055 survivors and 287 FDRs who were located, contacted, and agreed to participate, 59.5% and 62.7%, respectively, reported breast cancer screening in the past 12 months and were thus ineligible. For survivors enrolled at baseline (N = 95), the proportion reporting adherence to guideline screening by 12 months post-enrollment was similar in the high and low-intensity arms (66.7% vs. 79.2%, p = 0.31). Among FDRs enrolled at baseline (N = 83), screening was significantly higher in the high-intensity arm at 12 months (60.9% vs. 32.4%, p = 0.03). Overall, about 72% of study-eligible survivors (all of whom were screening nonadherent at baseline) reported screening within 12 months of study enrollment. For enrolled FDRs receiving the high-intensity intervention, over 60% reported guideline screening by 12 months. A major conclusion is that using high-quality central cancer registries to identify high-risk breast cancer survivors and then working closely with these survivors to identify their FDRs represents a feasible and effective strategy to promote guideline cancer screening.
Secondhand Smoke Exposure in Public Places and Support for Smoke-Free Laws in Japan: Findings from the 2018 ITC Japan Survey
Authors: Sansone Genevieve , Fong Geoffrey T. , Meng Gang , Craig Lorraine V. , Xu Steve S. , Quah Anne C. K. , Ouimet Janine , Mochizuki Yumiko , Yoshimi Itsuro , Tabuchi Takahiro
Published: February 2020

Comprehensive smoke-free policies such as those called for by the WHO FCTC are the only way to protect the public effectively from the harms of secondhand smoke (SHS), yet Japan has been slow to implement this important health measure. This study examines baseline levels of smoking and SHS exposure in public places and support for smoking bans in Japan prior to the implementation of the 2018 national smoke-free law. Data are from the International Tobacco Control (ITC) Japan Wave 1 Survey (Feb–Mar 2018), a web survey of adult cigarette smokers, heated tobacco product users, dual users, and non-users (total N = 4684). Measures included prevalence of smoking (whether respondents noticed people smoking inside restaurants and bars at their last visit, and workplaces in the last month), and support for complete smoking bans in these venues. Smoking prevalence in each venue was high overall in 2018 (49% of workplaces, 55% of restaurants, and 83% of bars), even higher than in China, the country with the greatest toll of SHS. Support for complete smoking bans was very high overall (81% for workplaces, 78% for restaurants, and 65% for bars). Non-users were less likely to be exposed to SHS and had higher support for smoking bans than tobacco users. These findings point to the ineffectiveness of partial smoke-free laws in Japan and reinforce the call for comprehensive smoke-free laws, which even smokers would support at higher levels than in many other ITC countries.
A Spatial Analysis of the Potentials for Offshore Wind Farm Locations in the North Sea Region: Challenges and Opportunities
Authors: Gusatu Laura Florentina , Yamu Claudia , Zuidema Christian , Faaij André
Published: February 2020

Over the last decade, the accelerated transition towards cleaner means of producing energy has been clearly prioritised by the European Union through large-scale planned deployment of wind farms in the North Sea. From a spatial planning perspective, this has not been a straight-forward process, due to substantial spatial conflicts with the traditional users of the sea, especially with fisheries and protected areas. In this article, we examine the availability of offshore space for wind farm deployment, from a transnational perspective, while taking into account different options for the management of the maritime area through four scenarios. We applied a mixed-method approach, combining expert knowledge and document analysis with the spatial visualisation of existing and future maritime spatial claims. Our calculations clearly indicate a low availability of suitable locations for offshore wind in the proximity of the shore and in shallow waters, even when considering its multi-use with fisheries and protected areas. However, the areas within 100 km from shore and with a water depth above –120 m attract greater opportunities for both single use (only offshore wind farms) and multi-use (mainly with fisheries), from an integrated planning perspective. On the other hand, the decrease of energy targets combined with sectoral planning result in clear limitations to suitable areas for offshore wind farms, indicating the necessity to consider areas with a water depth below –120 m and further than 100 km from shore. Therefore, despite the increased costs of maintenance and design adaptation, the multi-use of space can be a solution for more sustainable, stakeholder-engaged and cost-effective options in the energy deployment process. This paper identifies potential pathways, as well as challenges and opportunities for future offshore space management with the aim of achieving the 2050 renewable energy targets.
Increased Flavonol Levels in Tobacco Expressing AcFLS Affect Flower Color and Root Growth
Authors: Park Sangkyu , Kim Da-Hye , Yang Ju-Hee , Lee Jong-Yeol , Lim Sun-Hyung
Published: February 2020

The onion (Allium cepa L.) flavonol synthase (AcFLS-HRB) gene, encoding an enzyme responsible for flavonol biosynthesis in yellow onion, was recently identified and enzymatically characterized. Here, we performed an in vivo feeding assay involving bacterial expression of AcFLS-HRB and observed that it exhibited both flavanone 3-hydroxylase (F3H) and FLS activity. Transgenic tobacco (Nicotiana tabacum) expressing AcFLS-HRB produced lighter-pink flowers compared to wild-type plants. In transgenic petals, AcFLS-HRB was highly expressed at the mRNA and protein levels, and most AcFLS-HRB protein accumulated in the insoluble microsomal fractions. High-performance liquid chromatography (HPLC) analysis showed that flavonol levels increased but anthocyanin levels decreased in transgenic petals, indicating that AcFLS-HRB is a functional gene in planta. Gene expression analysis showed the reduced transcript levels of general phenylpropanoid biosynthetic genes and flavonoid biosynthetic genes in AcFLS-HRB overexpressed tobacco petals. Additionally, transgenic tobacco plants at the seedling stages showed increased primary root and root hair length and enhanced quercetin signals in roots. Exogenous supplementation with quercetin 3-O-rutinoside (rutin) led to the same phenotypic changes in root growth, suggesting that rutin is the causal compound that promotes root growth in tobacco. Therefore, augmenting flavonol levels affects both flower color and root growth in tobacco.
Evidence Implicating Non-Dioxin-Like Congeners as the Key Mediators of Polychlorinated Biphenyl (PCB) Developmental Neurotoxicity
Authors: Klocke Carolyn , Lein Pamela J.
Published: February 2020

Despite being banned from production for decades, polychlorinated biphenyls (PCBs) continue to pose a significant risk to human health. This is due to not only the continued release of legacy PCBs from PCB-containing equipment and materials manufactured prior to the ban on PCB production, but also the inadvertent production of PCBs as byproducts of contemporary pigment and dye production. Evidence from human and animal studies clearly identifies developmental neurotoxicity as a primary endpoint of concern associated with PCB exposures. However, the relative role(s) of specific PCB congeners in mediating the adverse effects of PCBs on the developing nervous system, and the mechanism(s) by which PCBs disrupt typical neurodevelopment remain outstanding questions. New questions are also emerging regarding the potential developmental neurotoxicity of lower chlorinated PCBs that were not present in the legacy commercial PCB mixtures, but constitute a significant proportion of contemporary human PCB exposures. Here, we review behavioral and mechanistic data obtained from experimental models as well as recent epidemiological studies that suggest the non-dioxin-like (NDL) PCBs are primarily responsible for the developmental neurotoxicity associated with PCBs. We also discuss emerging data demonstrating the potential for non-legacy, lower chlorinated PCBs to cause adverse neurodevelopmental outcomes. Molecular targets, the relevance of PCB interactions with these targets to neurodevelopmental disorders, and critical data gaps are addressed as well.
The FDA-Approved Anti-Asthma Medicine Ciclesonide Inhibits Lung Cancer Stem Cells through Hedgehog Signaling-Mediated SOX2 Regulation
Authors: Choi Hack Sun , Kim Su-Lim , Kim Ji-Hyang , Lee Dong-Sun
Published: February 2020

Ciclesonide is an FDA-approved glucocorticoid (GC) used to treat asthma and allergic rhinitis. However, its effects on cancer and cancer stem cells (CSCs) are unknown. Our study focuses on investigating the inhibitory effect of ciclesonide on lung cancer and CSCs and its underlying mechanism. In this study, we showed that ciclesonide inhibits the proliferation of lung cancer cells and the growth of CSCs. Similar glucocorticoids, such as dexamethasone and prednisone, do not inhibit CSC formation. We show that ciclesonide is important for CSC formation through the Hedgehog signaling pathway. Ciclesonide reduces the protein levels of GL1, GL2, and Smoothened (SMO), and a small interfering RNA (siRNA) targeting SMO inhibits tumorsphere formation. Additionally, ciclesonide reduces the transcript and protein levels of SOX2, and an siRNA targeting SOX2 inhibits tumorsphere formation. To regulate breast CSC formation, ciclesonide regulates GL1, GL2, SMO, and SOX2. Our results unveil a novel mechanism involving Hedgehog signaling and SOX2 regulated by ciclesonide in lung CSCs, and also open up the possibility of targeting Hedgehog signaling and SOX2 to prevent lung CSC formation.
JNK Signaling as a Key Modulator of Soft Connective Tissue Physiology, Pathology, and Healing
Authors: Nikoloudaki Georgia , Brooks Sarah , Peidl Alexander P. , Tinney Dylan , Hamilton Douglas W.
Published: February 2020

In healthy individuals, the healing of soft tissues such as skin after pathological insult or post injury follows a relatively predictable and defined series of cell and molecular processes to restore tissue architecture and function(s). Healing progresses through the phases of hemostasis, inflammation, proliferation, remodeling, and concomitant with re-epithelialization restores barrier function. Soft tissue healing is achieved through the spatiotemporal interplay of multiple different cell types including neutrophils, monocytes/macrophages, fibroblasts, endothelial cells/pericytes, and keratinocytes. Expressed in most cell types, c-Jun N-terminal kinases (JNK) are signaling molecules associated with the regulation of several cellular processes involved in soft tissue wound healing and in response to cellular stress. A member of the mitogen-activated protein kinase family (MAPK), JNKs have been implicated in the regulation of inflammatory cell phenotype, as well as fibroblast, stem/progenitor cell, and epithelial cell biology. In this review, we discuss our understanding of JNKs in the regulation of cell behaviors related to tissue injury, pathology, and wound healing of soft tissues. Using models as diverse as Drosophila, mice, rats, as well as human tissues, research is now defining important, but sometimes conflicting roles for JNKs in the regulation of multiple molecular processes in multiple different cell types central to wound healing processes. In this review, we focus specifically on the role of JNKs in the regulation of cell behavior in the healing of skin, cornea, tendon, gingiva, and dental pulp tissues. We conclude that while parallels can be drawn between some JNK activities and the control of cell behavior in healing, the roles of JNK can also be very specific modes of action depending on the tissue and the phase of healing.
Role of Stomatal Conductance in Modifying the Dose Response of Stress-Volatile Emissions in Methyl Jasmonate Treated Leaves of Cucumber (Cucumis Sativa)
Authors: Jiang Yifan , Ye Jiayan , Rasulov Bahtijor , Niinemets Ülo
Published: February 2020

Treatment by volatile plant hormone methyl jasmonate (MeJA) leads to release of methanol and volatiles of lipoxygenase pathway (LOX volatiles) in a dose-dependent manner, but how the dose dependence is affected by stomatal openness is poorly known. We studied the rapid (0–60 min after treatment) response of stomatal conductance (Gs), net assimilation rate (A), and LOX and methanol emissions to varying MeJA concentrations (0.2–50 mM) in cucumber (Cucumis sativus) leaves with partly open stomata and in leaves with reduced Gs due to drought and darkness. Exposure to MeJA led to initial opening of stomata due to an osmotic shock, followed by MeJA concentration-dependent reduction in Gs, whereas A initially decreased, followed by recovery for lower MeJA concentrations and time-dependent decline for higher MeJA concentrations. Methanol and LOX emissions were elicited in a MeJA concentration-dependent manner, whereas the peak methanol emissions (15–20 min after MeJA application) preceded LOX emissions (20–60 min after application). Furthermore, peak methanol emissions occurred earlier in treatments with higher MeJA concentration, while the opposite was observed for LOX emissions. This difference reflected the circumstance where the rise of methanol release partly coincided with MeJA-dependent stomatal opening, while stronger stomatal closure at higher MeJA concentrations progressively delayed peak LOX emissions. We further observed that drought-dependent reduction in Gs ameliorated MeJA effects on foliage physiological characteristics, underscoring that MeJA primarily penetrates through the stomata. However, despite reduced Gs, dark pretreatment amplified stress-volatile release upon MeJA treatment, suggesting that increased leaf oxidative status due to sudden illumination can potentiate the MeJA response. Taken together, these results collectively demonstrate that the MeJA dose response of volatile emission is controlled by stomata that alter MeJA uptake and volatile release kinetics and by leaf oxidative status in a complex manner.
Sphingolipid Signature of Human Feto-Placental Vasculature in Preeclampsia
Authors: Del Gaudio Ilaria , Sasset Linda , Di Lorenzo Annarita , Wadsack Christian
Published: February 2020

Bioactive sphingolipids are emerging as key regulators of vascular function and homeostasis. While most of the clinical studies have been devoted to profile circulating sphingolipids in maternal plasma, little is known about the role of the sphingolipid at the feto-placental vasculature, which is in direct contact with the offspring circulation. Our study aims to compare the sphingolipid profile of normal with preeclamptic (PE) placental chorionic arteries and isolated endothelial cells, with the goal of unveiling potential underlying pathomechanisms in the vasculature. Dihydrosphingosine and sphingomyelin (SM) concentrations (C16:0-, C18:0-, and C24:0- sphingomyelin) were significantly increased in chorionic arteries of preeclamptic placentas, whereas total ceramide, although showing a downward trend, were not statistically different. Moreover, RNA and immunofluorescence analysis showed impaired sphingosine-1-phosphate (S1P) synthesis and signaling in PE vessels. Our data reveal that the exposure to a deranged maternal intrauterine environment during PE alters the sphingolipid signature and gene expression on the fetal side of the placental vasculature. This pathological remodeling consists in increased serine palmitoyltransferase (SPT) activity and SM accrual in PE chorionic arteries, with concomitance impairment endothelial S1P signaling in the endothelium of these vessels. The increase of endothelial S1P phosphatase, lyase and S1PR2, and blunted S1PR1 expression support the onset of the pathological phenotype in chorionic arteries.
Antioxidant Role of PRGF on RPE Cells after Blue Light Insult as a Therapy for Neurodegenerative Diseases
Authors: Suárez-Barrio Carlota , del Olmo-Aguado Susana , García-Pérez Eva , de la Fuente María , Muruzabal Francisco , Anitua Eduardo , Baamonde-Arbaiza Begoña , Fernández-Vega-Cueto Luis , Fernández-Vega Luis , Merayo-Lloves Jesús
Published: February 2020

Oxidative stress has a strong impact on the development of retinal diseases such as age-related macular degeneration (AMD). Plasma rich in growth factors (PRGF) is a novel therapeutic approach in ophthalmological pathologies. The aim of this study was to analyze the antioxidant effect of PRGF in retinal epithelial cells (EPR) in in vitro and ex vivo retinal phototoxicity models. In vitro analyses were performed on ARPE19 human cell line. Viability and mitochondrial status were assessed in order to test the primary effects of PRGF. GSH level, and protein and gene expression of the main antioxidant pathway (Keap1, Nrf2, GCL, HO-1, and NQO1) were also studied. Ex vivo analyses were performed on rat RPE, and HO-1 and Nrf2 gene and protein expression were evaluated. The results show that PRGF reduces light insult by stimulating the cell response against oxidative damage and modulates the antioxidant pathway. We conclude that PRGF’s protective effect could prove useful as a new therapy for treating neurodegenerative disorders such as AMD.