bluuebunny/crossref_metadata_2025 huggingface dataset:
Promoting decentralized and flexible budgets in England: Lessons from the past and future prospects
Authors: Ayres Sarah , Stafford Ian
Published: September 2011

The UK has traditionally been viewed as a classic example of a unitary state in which central institutions dominate decision making. The recent Labour Government sought to counter this convention through devolution to Scotland, Wales, Northern Ireland and London and administrative decentralization to the English regions. This article examines New Labour’s efforts to promote sub-national policy discretion and fiscal autonomy via the Regional Funding Allocations (RFA) process. Findings are subsequently drawn upon to offer insights into the difficulties the Coalition Government is likely to face in its endeavor to decentralize functions and budgets to local authorities and communities. The paper addresses two central questions (i) Can New Labour’s attempt to promote decentralized and flexible budgets in England be viewed asevidence of a transition to a more fluid, multi-level form of governance? (ii)What lessons can be harnessed from the RFA experience in taking forward the Coalition government’s plans to promote fiscal discretion at the sub-national tier?It concludes that there are deep-rooted barriers in Whitehall that may limitthe freedoms and flexibilities pledged to local government and could undermine efforts to decentralize.
Repeated brain conventional MRI and SPECT evaluation in systemic lupus erythematosus patients with and without neuropsychiatric involvement: a follow up study
Authors: Castellino G , Bortoluzzi A , Padovan M , Borrelli M , Feggi L , Govoni M
Published: September 2011

Objectives: To assess the utility of a combined neuroimaging approach in the follow up of patients affected by systemic lupus erythematosus (SLE) with and without neuropsychiatric (NP) involvement. Methods: Patients who underwent a first combined brain conventional magnetic resonance imaging (cMRI) and single photon emission computed tomography (SPECT) and later repeated the same examinations between 2001 and 2008 were retrieved from a large database. Clinical and neuroimaging data were analysed and their relationships evaluated at baseline and at follow up. Results: Fifty SLE patients (38 with and 12 without NP involvement, mean age 36.8 yrs and mean disease duration at first instrumental evaluation 5.5 yrs) were enrolled. At baseline, the majority of them had a diffuse pattern of NP involvement. After a mean follow up period of 4 years all patients repeated neuroimaging and clinical evaluation. In 23 patients (22 with and 1 without NP manifestations at baseline) a new NP event occurred. Overall, neuroimaging remained unchanged or improved, but in some cases it worsened. No correlations were found between instrumental findings and clinical picture. Conclusions: In this study, the clinical features at baseline appeared to be a better predictor of future NP events than morphological and functional neuroimaging. Therefore the utility of repeating a combined instrumental evaluation (cMRI and SPECT) may be debatable especially for patients with diffuse NP involvement where the decision to perform serial combined neuroimaging examinations should be carefully assessed and based mainly on clinical judgement. Lupus (2011) 20, 1387–1395.
Greening a Chemistry Teaching Methods Course at the School of Educational Studies, Universiti Sains Malaysia
Authors: Karpudewan Mageswary , Hj Ismail Zurida , Mohamed Norita
Published: September 2011

Green chemistry is the design, development and implementation of chemical products and processes to reduce or eliminate the use of sub-stances hazardous to human health and the environment. This article reports on the integration of green chemistry and sustainable development concepts (SDCs) into an existing teaching methods course for chemistry pre-service teachers. Incorporation of SDCs with green chemistry enhances the interdisciplinary nature of green chemistry. It is in line with the course purpose of introducing instructional strategies for teaching chemistry at the secondary level. Through the experiences of hands-on activities, writing lesson plans and integration of SDC content into lectures and discussion, pre-service teachers learned how to integrate these elements into their own courses. The design of this course is an example of how to integrate green chemistry incorporated with SDCs into a teacher education curriculum.
Wave Propagation Control in Beams Through Periodic Multi-Branch Shunts
Authors: Airoldi L. , Ruzzene M.
Published: September 2011

Periodic, shunted piezoelectric patches are applied for the control of wave propagation in beams. The patches are shunted through resonant circuits featuring a single and multiple resonances. The periodic arrangement of shunted patches provides the beam with attenuation properties which depend on the resonant behavior of the shunts. This article specifically illustrates the effectiveness of periodic shunted patches for passive wave propagation control, and focuses on the application of multi-resonant circuits for the generation of multiple attenuation frequency ranges (bandgaps), which can be tuned to target several structural modes of the beam. A numerical model predicts the attenuation characteristics of the beam for different shunting configurations. The experimental implementation of the concept on a beam with five patches illustrates the effectiveness of the method in terms of its attenuation capabilities and its tunable characteristics.
Adaptive Sliding Mode Fault-Tolerant Control for Semi-Active Suspension Using Magnetorheological Dampers
Authors: Dong Xiaomin , Yu Miao , Guan Zhi
Published: September 2011

This study presents the vibration of an adaptive sliding mode fault-tolerant control for MR suspension system considering the partial fault of MR dampers. After formulating a full car dynamic model featuring four MR dampers, the fault model of the MR dampers due to the varying working temperature is derived. An adaptive sliding model fault-tolerant control strategy is then proposed after the occurrence of a fault. The performance of the proposed control scheme is evaluated and compared under the bump road condition and presented in time domain. The results show that significant gains are made in the presence of the partial fault of an MR damper. The control scheme could reduce the effect of the partial fault of the MR damper on system performance.
Learning through interaction in children with autism: Preliminary data from asocial-communication-based intervention
Authors: Casenhiser Devin M. , Shanker Stuart G. , Stieben Jim
Published: September 2011

The study evaluates a social-communication-based approach to autism intervention aimed at improving the social interaction skills of children with autism spectrum disorder. We report preliminary results from an ongoing randomized controlled trial of 51 children aged 2 years 0 months to 4 years 11 months. Participants were assigned to either a target treatment or community treatment group. Families in the target treatment group were given 2 hours of therapy and coaching each week in an intervention emphasizing social-interaction and the parent-child relationship. Children in the community treatment group received a variety of services averaging 3.9 hours per week. After 12 months, outcomes were measured to determine changes in the groups in social interaction and communication. In addition, a regression analysis was conducted to determine whether changes in social interaction skills were associated with language development. Results suggest that children in the treatment group made significantly greater gains in social interaction skills in comparison to the community treatment group, but no between-group differences were found for standard language assessments. Initiation of joint attention, involvement, and severity of language delay were found to be significantly associated with improvement of language skills in children with autism. Finally caregiver skills targeted by the intervention were found to be significantly associated with changes in children’s interaction skills.
Children’s weight and participation in organized sports
Authors: Quinto Romani Annette
Published: September 2011

Aims: Literature dealing with the impact of organized sports on children’s weight has been marked by a lack of consensus. A major weakness characterizing most of this research is a lack of proper measurement methods. This paper seeks to fill an important knowledge gap through careful application of econometric methods. Methods: Estimations are carried out using data on 1,400 children attending 6th grade in 2008 in the municipality of Aalborg, Denmark. We use standard ordinary least squares (OLS) and class fixed effects to explore the effect of sports participation on body mass index (BMI) as well as underweight, overweight and obesity. Results: Results indicate that participation in organized sports reduced BMI by 2.1%. Likewise it reduced the likelihood of being overweight by 8.2 percentage points and obese by 3.1 percentage points. Conclusions: It is the unique dataset combined with econometric methods that distinguishes our contribution from that of others in the field, thereby offering new insight. Results using statistically sound methods suggest that participation in organized sports has a beneficial effect on children’s weight.
Type D personality, depressive symptoms and work-related health outcomes
Authors: Mommersteeg Paula M.C. , Denollet Johan , Martens Elisabeth J.
Published: September 2011

Aims: Personality may play a decisive role in perceiving work-related characteristics as stressful, leading to adverse health outcomes. Persons with a Distressed personality (Type D) experience increased negative emotions while inhibiting these emotions in social situations. We investigated the role of Type D personality on adverse health outcomes, sick leave, burnout and disability pension. The mediating role of depressive symptoms on this relation was assessed because Type D represents a vulnerability factor for depression. Methods: In a cross-sectional community sample of the Dutch population ( n = 1,172) Type D personality was related to sick-leave (five categories of days per year), burnout, and disability pension, controlling for confounders. Results: Individuals with Type D personality reported more burnout (27% vs 8%), disability pension (32% vs 11%), and had an increased incidence of short-term sick leave (χ2 = 13.1, p = 0.011) as compared to non-Type D’s. Type D was significantly related to burnout (odds ratio (OR) = 4.16) and disability pension (OR = 2.62) independent of confounders. The Sobel test indicated significant mediation of depression on the relation between Type D personality and the work-related health outcomes. After mediation Type D personality remained significantly related to burnout, indicating a unique unshared effect. Conclusions: Type D personality is related to adverse health outcomes in the working population, mediated by depression, which warrants further research for this personality type.
Exploring emotional expressions on YouTube through the lens of media system dependency theory
Authors: Lee Chei Sian
Published: September 2011

This study extends current work by investigating the role social networking sites play in facilitating emotional expressions following the death of a public figure, Michael Jackson. Through the lens of Media System Dependency theory, I perform a qualitative content analysis on comments generated by users of YouTube. Results show that YouTube plays an important role in facilitating emotional expressions as the analysis uncovered a variety of emotions (i.e. sadness and grief, anger and frustration). I also identify other possible micro-level and macro-level effects which may have influenced these emotional responses. This study also found that emotional expressions differed between gender with respect to the passage of time, ongoing events in the environment, and negative reports about Jackson. The results indicate that users can depend on the content provided by YouTube to meet their emotional needs during the grieving process following the death of a public figure.
Mother as mother and mother as citizen: Mothers of combat soldiers on national network news
Authors: Slattery Karen , Garner Ana C.
Published: September 2011

This study examines national television news images of mothers of US combat soldiers during the first seven years of the Iraq War. News stories presented mothers as archetypal good mothers engaged in maternal work long after their children’s deployment. Some mothers were depicted as vocal vis-à-vis their position on the Iraq War, a contrast to the historical depiction of the archetypal patriotic mother who is stoic and silent. The resulting image is more complex, suggesting the boundaries of the archetype may be changing.
New Technologies and Surgical Innovation
Authors: Poulin Paule , Austen Lea , Kortbeek John B. , Lafrenière René
Published: September 2011

There is pressure for surgical departments to introduce new and innovative health technologies in an evidence-based manner while ensuring that they are safe and effective and can be managed with available resources. A local health technology assessment (HTA) program was developed to systematically integrate research evidence with local operational management information and to make recommendations for subsequent decision by the departmental executive committee about whether and under what conditions the technology will be used. The authors present a retrospective analysis of the outcomes of this program as used by the Department of Surgery & Surgical Services in the Calgary Health Region over a 5-year period from December 2005 to December 2010. Of the 68 technologies requested, 15 applications were incomplete and dropped, 12 were approved, 3 were approved for a single case on an urgent/emergent basis, 21 were approved for “clinical audit” for a restricted number of cases with outcomes review, 14 were approved for research use only, and 3 were referred to additional review bodies. Subsequent outcome reports resulted in at least 5 technologies being dropped for failure to perform. Decisions based on local HTA program recommendations were rarely “yes” or “no.” Rather, many technologies were given restricted approval with full approval contingent on satisfying certain conditions such as clinical outcomes review, training protocol development, or funding. Thus, innovation could be supported while ensuring safety and effectiveness. This local HTA program can be adapted to a variety of settings and can help bridge the gap between evidence and practice.
Inhibitory effect of 10-hydroxy-trans-2-decenoic acid on LPS-induced IL-6 production via reducing IκB-ζ expression
Authors: Sugiyama Tsuyoshi , Takahashi Keita , Tokoro Shunji , Gotou Takaki , Neri Paola , Mori Hiroshi
Published: September 2011

The effect of 10-hydroxy- trans-2-decenoic acid (10H2DA), a major fatty acid component of royal jelly, was investigated on LPS-induced cytokine production in murine macrophage cell line, RAW264 cells. 10H2DA inhibited LPS-induced IL-6 production dose-dependently, but did not inhibit TNF-α production. 10H2DA inhibited LPS-induced NF-κB activation in a dose-dependent fashion. In addition, NF-κB activation induced by over-expression of either MyD88 or Toll/IL-1 receptor domain-containing adaptor inducing IFN-β (TRIF) was also inhibited by 10H2DA. Degradation of IκB-α and phosphorylation of IκB kinase-α were not inhibited by 10H2DA. On the other hand, reduction of LPS-induced IκB-ζ expression was discovered. Production of lipocalin-2 and granulocyte colony-stimulating factor (G-CSF), which is dependent on IκB-ζ, was also inhibited by 10H2DA, whereas that of IκB-ζ–independent cytokines/chemokines, such as IFN-β, murine monocyte chemotactic protein-1 (JE), macrophage inflammatory protein (MIP)-1α and MIP-2, was not. Together, 10H2DA specifically inhibited LPS-induced IκB-ζ expression, followed by inhibition of IκB-ζ-dependent gene production. These results suggest that 10H2DA is one of the components of royal jelly to show anti-inflammatory effects and could be a therapeutic drug candidate for inflammatory and autoimmune diseases associated with IκB-ζ and IL-6 production.
Preemptive immunotherapy in childhood acute myeloid leukemia for patients showing evidence of mixed chimerism after allogeneic stem cell transplantation
Authors: Rettinger Eva , Willasch Andre M. , Kreyenberg Hermann , Borkhardt Arndt , Holter Wolfgang , Kremens Bernhard , Strahm Brigitte , Woessmann Wilhelm , Mauz-Koerholz Christine , Gruhn Bernd , Burdach Stefan , Albert Michael H. , Schlegel Paul-Gerhardt , Klingebiel Thomas , Bader Peter
Published: September 2011

Previous studies have shown that children with acute myeloid leukemia (AML) who developed mixed chimerism (MC) were at high risk for relapse after allogeneic stem-cell transplantation (allo-SCT). We investigated the feasibility of intensified preemptive immunotherapy in children receiving allo-SCT for AML. Eighty-four children were registered in our trial from May 2005 to April 2009; of these, 71 fulfilled the inclusion criteria and were treated according to the study protocol. Serial and semiquantitative analyses of posttransplantation chimerism were performed. Defined immunotherapy approaches were considered in MC patients. Continuous complete chimerism (CC) was observed in 51 of 71 patients. MC was detected in 20 patients and was followed by immunotherapy in 13. Six of 13 MC patients returned to CC without toxicity and remained in long-term remission. Overall, the probability of event-free survival (pEFS) was 66% (95% confidence interval [95% CI] = 53%-76%) for all patients and 46% (95% CI = 19%-70%) in MC patients with intervention; however, this number increased to 71% (95% CI = 26%-92%) in 7 of 13 MC patients on immunotherapy who were in remission at the time of transplantation. All MC patients without intervention relapsed. These results suggest that MC is a prognostic factor for impending relapse in childhood AML, and that preemptive immunotherapy may improve the outcome in defined high-risk patients after transplantation.
Increase of cells expressing PD-L1 in bovine leukemia virus infection and enhancement of anti-viral immune responses in vitro via PD-L1 blockade
Authors: Ikebuchi Ryoyo , Konnai Satoru , Shirai Tatsuya , Sunden Yuji , Murata Shiro , Onuma Misao , Ohashi Kazuhiko
Published: September 2011

The inhibitory receptor programmed death-1 (PD-1) and its ligand, programmed death-ligand 1 (PD-L1) are involved in immune evasion mechanisms for several pathogens causing chronic infections. Blockade of the PD-1/PD-L1 pathway restores anti-virus immune responses, with concomitant reduction in viral load. In a previous report, we showed that, in bovine leukemia virus (BLV) infection, the expression of bovine PD-1 is closely associated with disease progression. However, the functions of bovine PD-L1 are still unknown. To investigate the role of PD-L1 in BLV infection, we identified the bovine PD-L1 gene, and examined PD-L1 expression in BLV-infected cattle in comparison with uninfected cattle. The deduced amino acid sequence of bovine PD-L1 shows high homology to the human and mouse PD-L1. The proportion of PD-L1 positive cells, especially among B cells, was upregulated in cattle with the late stage of the disease compared to cattle at the aleukemic infection stage or uninfected cattle. The proportion of PD-L1 positive cells correlated positively with prediction markers for the progression of the disease such as leukocyte number, virus load and virus titer whilst on the contrary, it inversely correlated with the degree of interferon-gamma expression. Blockade of the PD-1/PD-L1 pathway in vitro by PD-L1-specific antibody upregulated the production of interleukin-2 and interferon-gamma, and correspondingly, downregulated the BLV provirus load and the proportion of BLV-gp51 expressing cells. These data suggest that PD-L1 induces immunoinhibition in disease progressed cattle during chronic BLV infection. Therefore, PD-L1 would be a potential target for developing immunotherapies against BLV infection.
Detailed kinetics and regulation of mammalian 2-oxoglutarate dehydrogenase
Authors: Qi Feng , Pradhan Ranjan K , Dash Ranjan K , Beard Daniel A
Published: September 2011

Background

Mitochondrial 2-oxoglutarate (α-ketoglutarate) dehydrogenase complex (OGDHC), a key regulatory point of tricarboxylic acid (TCA) cycle, plays vital roles in multiple pathways of energy metabolism and biosynthesis. The catalytic mechanism and allosteric regulation of this large enzyme complex are not fully understood. Here computer simulation is used to test possible catalytic mechanisms and mechanisms of allosteric regulation of the enzyme by nucleotides (ATP, ADP), pH, and metal ion cofactors (Ca2+ and Mg2+).

Results

A model was developed based on an ordered ter-ter enzyme kinetic mechanism combined with con-formational changes that involve rotation of one lipoic acid between three catalytic sites inside the enzyme complex. The model was parameterized using a large number of kinetic data sets on the activity of OGDHC, and validated by comparison of model predictions to independent data.

Conclusions

The developed model suggests a hybrid rapid-equilibrium ping-pong random mechanism for the kinetics of OGDHC, consistent with previously reported mechanisms, and accurately describes the experimentally observed regulatory effects of cofactors on the OGDHC activity. This analysis provides a single consistent theoretical explanation for a number of apparently contradictory results on the roles of phosphorylation potential, NAD (H) oxidation-reduction state ratio, as well as the regulatory effects of metal ions on ODGHC function.
Prediction of protein-binding areas by small-world residue networks and application to docking
Authors: Pons Carles , Glaser Fabian , Fernandez-Recio Juan
Published: September 2011

Background

Protein-protein interactions are involved in most cellular processes, and their detailed physico-chemical and structural characterization is needed in order to understand their function at the molecular level. In-silico docking tools can complement experimental techniques, providing three-dimensional structural models of such interactions at atomic resolution. In several recent studies, protein structures have been modeled as networks (or graphs), where the nodes represent residues and the connecting edges their interactions. From such networks, it is possible to calculate different topology-based values for each of the nodes, and to identify protein regions with high centrality scores, which are known to positively correlate with key functional residues, hot spots, and protein-protein interfaces.

Results

Here we show that this correlation can be efficiently used for the scoring of rigid-body docking poses. When integrated into the pyDock energy-based docking method, the new combined scoring function significantly improved the results of the individual components as shown on a standard docking benchmark. This improvement was particularly remarkable for specific protein complexes, depending on the shape, size, type, or flexibility of the proteins involved.

Conclusions

The network-based representation of protein structures can be used to identify protein-protein binding regions and to efficiently score docking poses, complementing energy-based approaches.
iCTNet: A Cytoscape plugin to produce and analyze integrative complex traits networks
Authors: Wang Lili , Khankhanian Pouya , Baranzini Sergio E , Mousavi Parvin
Published: September 2011

Background

The speed at which biological datasets are being accumulated stands in contrast to our ability to integrate them meaningfully. Large-scale biological databases containing datasets of genes, proteins, cells, organs, and diseases are being created but they are not connected. Integration of these vast but heterogeneous sources of information will allow the systematic and comprehensive analysis of molecular and clinical datasets, spanning hundreds of dimensions and thousands of individuals. This integration is essential to capitalize on the value of current and future molecular- and cellular-level data on humans to gain novel insights about health and disease.

Results

We describe a new open-source Cytoscape plugin named iCTNet (i ntegrated C omplex T raits Net works). iCTNet integrates several data sources to allow automated and systematic creation of networks with up to five layers of omics information: phenotype-SNP association, protein-protein interaction, disease-tissue, tissue-gene, and drug-gene relationships. It facilitates the generation of general or specific network views with diverse options for more than 200 diseases. Built-in tools are provided to prioritize candidate genes and create modules of specific phenotypes.

Conclusions

iCTNet provides a user-friendly interface to search, integrate, visualize, and analyze genome-scale biological networks for human complex traits. We argue this tool is a key instrument that facilitates systematic integration of disparate large-scale data through network visualization, ultimately allowing the identification of disease similarities and the design of novel therapeutic approaches.

The online database and Cytoscape plugin are freely available for academic use at: http://www.cs.queensu.ca/ictnet
Widespread Horizontal Gene Transfer from Circular Single-stranded DNA Viruses to Eukaryotic Genomes
Authors: Liu Huiquan , Fu Yanping , Li Bo , Yu Xiao , Xie Jiatao , Cheng Jiasen , Ghabrial Said A , Li Guoqing , Yi Xianhong , Jiang Daohong
Published: September 2011

Background

In addition to vertical transmission, organisms can also acquire genes from other distantly related species or from their extra-chromosomal elements (plasmids and viruses) via horizontal gene transfer (HGT). It has been suggested that phages represent substantial forces in prokaryotic evolution. In eukaryotes, retroviruses, which can integrate into host genome as an obligate step in their replication strategy, comprise approximately 8% of the human genome. Unlike retroviruses, few members of other virus families are known to transfer genes to host genomes.

Results

Here we performed a systematic search for sequences related to circular single-stranded DNA (ssDNA) viruses in publicly available eukaryotic genome databases followed by comprehensive phylogenetic analysis. We conclude that the replication initiation protein (Rep)-related sequences of geminiviruses, nanoviruses and circoviruses have been frequently transferred to a broad range of eukaryotic species, including plants, fungi, animals and protists. Some of the transferred viral genes were conserved and expressed, suggesting that these genes have been coopted to assume cellular functions in the host genomes. We also identified geminivirus-like and parvovirus-like transposable elements in genomes of fungi and lower animals, respectively, and thereby provide direct evidence that eukaryotic transposons could derive from ssDNA viruses.

Conclusions

Our discovery extends the host range of circular ssDNA viruses and sheds light on the origin and evolution of these viruses. It also suggests that ssDNA viruses act as an unforeseen source of genetic innovation in their hosts.
Genome wide gene expression regulation by HIP1 Protein Interactor, HIPPI: Prediction and validation
Authors: Datta Moumita , Choudhury Ananyo , Lahiri Ansuman , Bhattacharyya Nitai P
Published: September 2011

Background

HIP1 Protein Interactor (HIPPI) is a pro-apoptotic protein that induces Caspase8 mediated apoptosis in cell. We have shown earlier that HIPPI could interact with a specific 9 bp sequence motif, defined as the HIPPI binding site (HBS), present in the upstream promoter of Caspase1 gene and regulate its expression. We also have shown that HIPPI, without any known nuclear localization signal, could be transported to the nucleus by HIP1, a NLS containing nucleo-cytoplasmic shuttling protein. Thus our present work aims at the investigation of the role of HIPPI as a global transcription regulator.

Results

We carried out genome wide search for the presence of HBS in the upstream sequences of genes. Our result suggests that HBS was predominantly located within 2 Kb upstream from transcription start site. Transcription factors like CREBP1, TBP, OCT1, EVI1 and P53 half site were significantly enriched in the 100 bp vicinity of HBS indicating that they might co-operate with HIPPI for transcription regulation. To illustrate the role of HIPPI on transcriptome, we performed gene expression profiling by microarray. Exogenous expression of HIPPI in HeLa cells resulted in up-regulation of 580 genes (p < 0.05) while 457 genes were down-regulated. Several transcription factors including CBP, REST, C/EBP beta were altered by HIPPI in this study. HIPPI also interacted with P53 in the protein level. This interaction occurred exclusively in the nuclear compartment and was absent in cells where HIP1 was knocked down. HIPPI-P53 interaction was necessary for HIPPI mediated up-regulation of Caspase1 gene. Finally, we analyzed published microarray data obtained with post mortem brains of Huntington's disease (HD) patients to investigate the possible involvement of HIPPI in HD pathogenesis. We observed that along with the transcription factors like CREB, P300, SREBP1, Sp1 etc. which are already known to be involved in HD, HIPPI binding site was also significantly over-represented in the upstream sequences of genes altered in HD.

Conclusions

Taken together, the results suggest that HIPPI could act as an important transcription regulator in cell regulating a vast array of genes, particularly transcription factors and at least, in part, play a role in transcription deregulation observed in HD.
Analyses of the oligopeptide transporter gene family in poplar and grape
Authors: Cao Jun , Huang Jinling , Yang Yongping , Hu Xiangyang
Published: September 2011

Background

Oligopeptide transporters (OPTs) are a group of membrane-localized proteins that have a broad range of substrate transport capabilities and that are thought to contribute to many biological processes. The OPT proteins belong to a small gene family in plants, which includes about 25 members in Arabidopsis and rice. However, no comprehensive study incorporating phylogeny, chromosomal location, gene structure, expression profiling, functional divergence and selective pressure analysis has been reported thus far for Populus and Vitis.

Results

In the present study, a comprehensive analysis of the OPT gene family in Populus (P. trichocarpa) and Vitis (V. vinifera) was performed. A total of 20 and 18 full-length OPT genes have been identified in Populus and Vitis, respectively. Phylogenetic analyses indicate that these OPT genes consist of two classes that can be further subdivided into 11 groups. Gene structures are considerably conserved among the groups. The distribution of OPT genes was found to be non-random across chromosomes. A high proportion of the genes are preferentially clustered, indicating that tandem duplications may have contributed significantly to the expansion of the OPT gene family. Expression patterns based on our analyses of microarray data suggest that many OPT genes may be important in stress response and functional development of plants. Further analyses of functional divergence and adaptive evolution show that, while purifying selection may have been the main force driving the evolution of the OPTs, some of critical sites responsible for the functional divergence may have been under positive selection.

Conclusions

Overall, the data obtained from our investigation contribute to a better understanding of the complexity of the Populus and Vitis OPT gene family and of the function and evolution of the OPT gene family in higher plants.
Clinical features of headache associated with mobile phone use: a cross-sectional study in university students
Authors: Chu Min Kyung , Song Hoon Geun , Kim Chulho , Lee Byung Chul
Published: September 2011

Background

Headache has been reported to be associated with mobile phone (MP) use in some individuals. The causal relationship between headache associated with MP use (HAMP) and MP use is currently undetermined. Identifying the clinical features of HAMP may help in clarifying the pathophysiology of HAMP and in managing symptoms of individuals with HAMP. The aim of the present study is to describe the clinical features of HAMP.

Methods

A 14-item questionnaire investigating MP use and headache was administered to 247 medical students at Hallym University, Korea. Individual telephone interviews were subsequently conducted with those participants who reported HAMP more than 10 times during the last 1 year on the clinical features of HAMP. We defined HAMP as a headache attack during MP use or within 1 hour after MP use.

Results

In total, 214 (86.6%) students completed and returned the questionnaire. Forty (18.9%) students experienced HAMP more than 10 times during the last 1 year in the questionnaire survey. In subsequent telephone interviews, 37 (97.4%) interviewed participants reported that HAMP was triggered by prolonged MP use. HAMP was usually dull or pressing in quality (30 of 38, 79.0%), localised ipsilateral to the side of MP use (32 of 38, 84.2%), and associated with a burning sensation (24 of 38, 63.2%).

Conclusion

We found that HAMP usually showed stereotyped clinical features including mild intensity, a dull or pressing quality, localisation ipsilateral to the side of MP use, provocation by prolonged MP use and often accompanied by a burning sensation.
Aurora kinases are expressed in medullary thyroid carcinoma (MTC) and their inhibition suppresses in vitro growth and tumorigenicity of the MTC derived cell line TT
Authors: Baldini Enke , Arlot-Bonnemains Yannick , Sorrenti Salvatore , Mian Caterina , Pelizzo Maria R , De Antoni Enrico , Palermo Silvio , Morrone Stefania , Barollo Susi , Nesca Angela , Moretti Costanzo G , D'Armiento Massimino , Ulisse Salvatore
Published: September 2011

Background

The Aurora kinase family members, Aurora-A, -B and -C, are involved in the regulation of mitosis, and alterations in their expression are associated with cell malignant transformation. To date no information on the expression of these proteins in medullary thyroid carcinoma (MTC) are available. We here investigated the expression of the Aurora kinases in human MTC tissues and their potential use as therapeutic targets.

Methods

The expression of the Aurora kinases in 26 MTC tissues at different TNM stages was analyzed at the mRNA level by quantitative RT-PCR. We then evaluated the effects of the Aurora kinase inhibitor MK-0457 on the MTC derived TT cell line proliferation, apoptosis, soft agar colony formation, cell cycle and ploidy.

Results

The results showed the absence of correlation between tumor tissue levels of any Aurora kinase and tumor stage indicating the lack of prognostic value for these proteins. Treatment with MK-0457 inhibited TT cell proliferation in a time- and dose-dependent manner with IC50 = 49.8 ± 6.6 nM, as well as Aurora kinases phosphorylation of substrates relevant to the mitotic progression. Time-lapse experiments demonstrated that MK-0457-treated cells entered mitosis but were unable to complete it. Cytofluorimetric analysis confirmed that MK-0457 induced accumulation of cells with ≥ 4N DNA content without inducing apoptosis. Finally, MK-0457 prevented the capability of the TT cells to form colonies in soft agar.

Conclusions

We demonstrate that Aurora kinases inhibition hampered growth and tumorigenicity of TT cells, suggesting its potential therapeutic value for MTC treatment.
Decreased expression of dual-specificity phosphatase 9 is associated with poor prognosis in clear cell renal cell carcinoma
Authors: Wu Song , Wang Yong , Sun Liang , Zhang Zhiling , Jiang Zhimao , Qin Zike , Han Hui , Liu Zhuowei , Li Xianxin , Tang Aifa , Gui Yaoting , Cai Zhiming , Zhou Fangjian
Published: September 2011

Background

The molecular mechanisms involved in the development and progression of clear cell renal cell carcinomas (ccRCCs) are poorly understood. The objective of this study was to analyze the expression of dual-specificity phosphatase 9 (DUSP-9) and determine its clinical significance in human ccRCCs.

Methods

The expression of DUSP-9 mRNA was determined in 46 paired samples of ccRCCs and adjacent normal tissues by using real-time qPCR. The expression of the DUSP-9 was determined in 211 samples of ccRCCs and 107 paired samples of adjacent normal tissues by immunohistochemical analysis. Statistical analysis was performed to define the relationship between the expression of DUSP-9 and the clinical features of ccRCC.

Results

The mRNA level of DUSP-9, which was determined by real-time RT-PCR, was found to be significantly lower in tumorous tissues than in the adjacent non-tumorous tissues (p < 0.001). An immunohistochemical analysis of 107 paired tissue specimens showed that the DUSP-9 expression was lower in tumorous tissues than in the adjacent non-tumorous tissues (p < 0.001). Moreover, there was a significant correlation between the DUSP-9 expression in ccRCCs and gender (p = 0.031), tumor size (p = 0.001), pathologic stage (p = 0.001), Fuhrman grade (p = 0.002), T stage (p = 0.001), N classification (p = 0.012), metastasis (p = 0.005), and recurrence (p < 0.001). Patients with lower DUSP-9 expression had shorter overall survival time than those with higher DUSP-9 expression (p < 0.001). Multivariate analysis indicated that low expression of the DUSP-9 was an independent predictor for poor survival of ccRCC patients.

Conclusion

To our knowledge, this is the first study that determines the relationship between DUSP-9 expression and prognosis in ccRCC. We found that decreased expression of DUSP-9 is associated with poor prognosis in ccRCC. DUSP-9 may represent a novel and useful prognostic marker for ccRCC.
The extramural metastasis might be categorized in lymph node staging for colorectal cancer
Authors: Qiu Hai-Bo , Chen Gong , Keshari Rajiv P , Luo Hui-Yan , Fang Wang , Qiu Miao-Zhen , Zhou Zhi-Wei , Xu Rui-Hua
Published: September 2011

Background

The objective of this study is to assess the clinical significance and prognostic impact of extramural metastasis in colorectal carcinoma and establish an optimal categorization in the staging system.

Methods

To determine the frequency and prognostic significance of extramural metastasis, from 2000 to 2005, a total of 1,215 patients with colorectal cancer who underwent surgical resection were recruited into this study. Individual demographic and clinicopathologic data were collected including tumor stage, nodal stage, tumor histology, degree of tumor differentiation, and presence of lymphovascular invasion. After surgery, all patients received standard treatments and follow-up, which were closed in April 2010.

Results

EM was detected in 167 (13.7%) patients and in 230 (1.8%) of the 12,534 nodules retrieved as 'lymph nodes'. The incidence of extramural metastasis was significantly higher in patients with large tumors, deeper invasive depth and more lymph node metastasis (P < 0.001). After curative operation, overall survival was significantly worse for patients with extramural metastasis than those without (P < 0.001). Multivariate analysis identified extramural metastasis as an independent prognostic factor (RR = 2.1, 95%CI:1.5-3.0). By using the Akaike information criterion (AIC), N staging was capable of predicting survival outcome with the highest accuracy when both nodal involvement and extramural metastasis were treated together as N factors(AIC = 1025.3).

Conclusion

Extramural metastasis might be diagnosed as replaced lymph nodes in the process of classification, thus forming a new categorization.
Hyperhomocysteinemia is independently associated with albuminuria in the population-based CoLaus study
Authors: Marti Franziska , Vollenweider Peter , Marques-Vidal Pedro-Manuel , Mooser Vincent , Waeber Gérard , Paccaud Fred , Bochud Murielle
Published: September 2011

Background

Increased serum levels of homocysteine and uric acid have each been associated with cardiovascular risk. We analyzed whether homocysteine and uric acid were associated with glomerular filtration rate (GFR) and albuminuria independently of each other. We also investigated the association of MTHFR polymorphisms related to homocysteine with albuminuria to get further insight into causality.

Methods

This was a cross-sectional population-based study in Caucasians (n = 5913). Hyperhomocysteinemia was defined as total serum homocysteine ≥ 15 μmol/L. Albuminuria was defined as urinary albumin-to-creatinine ratio > 30 mg/g.

Results

Uric acid was associated positively with homocysteine (r = 0.246 in men and r = 0.287 in women, P < 0.001). The prevalence of albuminuria increased across increasing homocysteine categories (from 6.4% to 17.3% in subjects with normal GFR and from 3.5% to 14.5% in those with reduced GFR, P for trend < 0.005). Hyperhomocysteinemia (OR = 2.22, 95% confidence interval: 1.60-3.08, P < 0.001) and elevated serum uric acid (OR = 1.27, 1.08-1.50, per 100 μmol/L, P = 0.004) were significantly associated with albuminuria, independently of hypertension and type 2 diabetes. The 2-fold higher risk of albuminuria associated with hyperhomocysteinemia was similar to the risk associated with hypertension or diabetes. MTHFR alleles related to higher homocysteine were associated with increased risk of albuminuria.

Conclusions

In the general adult population, elevated serum homocysteine and uric acid were associated with albuminuria independently of each other and of renal function.
A survey of current and past Pediatric Infectious Diseases fellows regarding training
Authors: Douvoyiannis Miltiadis , Litman Nathan , Belamarich Peter F , Goldman David L
Published: September 2011

Background

The objectives of this study were to characterize the satisfaction of Pediatric Infectious Diseases fellows with their training and to understand how opinions about training have changed over time.

Methods

Anonymous survey studies were conducted with questions designed to include areas related to the 6 ACGME core competencies. Surveys for current fellows were distributed by fellowship directors, while surveys for graduates were mailed to all individuals with Pediatric Infectious Diseases certification.

Results

Response rates for current fellows and graduates were 50% and 52%, respectively. Most fellows (98%) and graduates (92%) perceived their overall training favorably. Training in most clinical care areas was rated favorably, however both groups perceived relative deficiencies in several areas. Current fellows rated their training in other competency areas (e.g., systems-based practice, research, and ethics) more favorably when compared to past graduates. Recent graduates perceived their training more favorably in many of these areas compared to past graduates.

Conclusions

Pediatric Infectious Diseases fellowship training is well regarded by the majority of current and past trainees. Views of current fellows reflect improved satisfaction with training in a variety of competency areas. Persistent deficiencies in clinical training likely reflect active barriers to education. Additional study is warranted to validate perceived deficiencies and to establish consensus on the importance of these areas to infectious diseases training.
Risk communication and informed consent in the medical tourism industry: A thematic content analysis of canadian broker websites
Authors: Penney Kali , Snyder Jeremy , Crooks Valorie A , Johnston Rory
Published: September 2011

Background

Medical tourism, thought of as patients seeking non-emergency medical care outside of their home countries, is a growing industry worldwide. Canadians are amongst those engaging in medical tourism, and many are helped in the process of accessing care abroad by medical tourism brokers - agents who specialize in making international medical care arrangements for patients. As a key source of information for these patients, brokers are likely to play an important role in communicating the risks and benefits of undergoing surgery or other procedures abroad to their clientele. This raises important ethical concerns regarding processes such as informed consent and the liability of brokers in the event that complications arise from procedures. The purpose of this article is to examine the language, information, and online marketing of Canadian medical tourism brokers' websites in light of such ethical concerns.

Methods

An exhaustive online search using multiple search engines and keywords was performed to compile a comprehensive directory of English-language Canadian medical tourism brokerage websites. These websites were examined using thematic content analysis, which included identifying informational themes, generating frequency counts of these themes, and comparing trends in these counts to the established literature.

Results

Seventeen websites were identified for inclusion in this study. It was found that Canadian medical tourism broker websites varied widely in scope, content, professionalism and depth of information. Three themes emerged from the thematic content analysis: training and accreditation, risk communication, and business dimensions. Third party accreditation bodies of debatable regulatory value were regularly mentioned on the reviewed websites, and discussion of surgical risk was absent on 47% of the websites reviewed, with limited discussion of risk on the remaining ones. Terminology describing brokers' roles was somewhat inconsistent across the websites. Finally, brokers' roles in follow up care, their prices, and the speed of surgery were the most commonly included business dimensions on the reviewed websites.

Conclusion

Canadian medical tourism brokers currently lack a common standard of care and accreditation, and are widely lacking in providing adequate risk communication for potential medical tourists. This has implications for the informed consent and consequent safety of Canadian medical tourists.
Alterations of serum biomarkers associated with lung ventilation function impairment in coal Workers: A cross-sectional study
Authors: Zou Jimin , du Prel Carroll Xianming , Liang Xianhong , Wang Dongmei , Li Chao , Yuan Baojun , Leeper-Woodford Sandra
Published: September 2011

Background

Previous studies have demonstrated that alterations in certain circulating biomarkers may be correlated with Coal workers' pneumoconiosis (CWP). This study investigated the relationship between changes of serum biomarkers and pulmonary function during the development of CWP.

Methods

Lung function parameters and specific serum indices were measured in 69 non-smoking coal workers, including 34 miners with CWP, 24 asymptomatic miners and 11 miners with minimal symptoms. The associations between changes in pulmonary function and serum indices were tested with Pearson's correlation coefficients. Multivariable analysis was used to estimate the predictive power of potential determinant variables for lung function.

Results

Compared to healthy miners, lung function (FVC, FEV1, FEF50, FEF75, FEF25-75% of predicted values) was decreased in miners with CWP (p< 0.05). Increased serum matrix metalloproteinase-9 (MMP-9) was associated with decreased FVC% of predicted values in the asymptomatic miners (r= -0.503,p= 0.014).

Conclusions

In coal mine workers, alterations of lung function parameters are associated with the development of CWP and with changes in circulating MMP-9, TIMP-9, IL-13 and IL-18R. These serum biomarkers may likely reflect the pathogenesis and progression of CWP in coal workers, and may provide for the importance of serum indicators in the early diagnosis of lung function injury in coal miners.