bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Reservoir Quality and Forecasted Production Variability along a Multi-Fractured Horizontal Well. Part 1: Reservoir Characterization
Authors: Becerra Daniela , Clarkson Christopher R. , Ghanizadeh Amin , Pires de Lima Rafael , Tabasinejad Farshad , Zhang Zhenzihao , Trivedi Ajesh , Shor Roman
Published: September 2021

Completion design for horizontal wells is typically performed using a geometric approach where the fracturing stages are evenly distributed along the lateral length of the well. However, this approach ignores the intrinsic vertical and horizontal heterogeneity of unconventional reservoirs, resulting in uneven production from hydraulic fracturing stages. An alternative approach is to selectively complete intervals with similar and superior reservoir quality (RQ) and completion quality (CQ), potentially leading to improved development efficiency. In the current study, along-well reservoir characterization is performed using data from a horizontal well completed in the Montney Formation in western Canada. Log-derived petrophysical and geomechanical properties, and laboratory analyses performed on drill cuttings, are integrated for the purpose of evaluating RQ and CQ variability along the well. For RQ, cutoffs were applied to the porosity (>4%), permeability (>0.0018 mD), and water saturation (<20%), whereas, for CQ, cutoffs were applied to rock strength (<160 Mpa), Young’s Modulus (60–65 GPa), and Poisson’s ratio (<0.26). Based on the observed heterogeneity in reservoir properties, the lateral length of the well can be subdivided into nine segments. Superior RQ and CQ intervals were found to be associated with predominantly (massive) porous siltstone facies; these intervals are regarded as the primary targets for stimulation. In contrast, relatively inferior RQ and CQ intervals were found to be associated with either dolomite-cemented facies or laminated siltstones. The methods developed and used in this study could be beneficial to Montney operators who aim to better predict and target sweet spots along horizontal wells; the approach could also be used in other unconventional plays.
Ultrasounds Energy as an Agent of Polyelectrolyte Modification Prior to Sewage Sludge Conditioning
Authors: Wolny Lidia , Wolski Paweł
Published: September 2021

The presented research concerned the phenomenon of polyelectrolyte changes resulting from modification by applying the ultrasonic field. The main aim of this research was to determine the activation degree of this macromolecular chemical compound and its effect on sewage sludge subjected to conditioning and followed by dewatering. The overall goal was to investigate the potential way of reducing the dosage of chemical compounds prior to sewage sludge conditioning. The polyelectrolyte samples were sonicated with the ultrasonic disintegrator UD-20 coupled with a sandwich concentrator. The power output of the generator was 180 W and the ultrasonic field frequency was 22 kHz. To describe the geometrical characteristics of the separated phases, the following parameters were determined: surface area (AA), perimeter (LA) and non-dimensional coefficient. With reference to the obtained results, the most significant quantitative changes in shape and size of the separated phases were observed for the ultrasonic field exposure time in the range of 0 to 10 s. This was in agreement with the results observed during dewatering of the investigated sewage sludge. In view of the quantitative analysis of the structure of the polyelectrolyte subjected to the ultrasonic modification, dewatering of sewage sludge was considerably improved by the application of the presented method.
Analysis of the Noise Pollution in the Bielański Forest NATURA 2000 Area in Light of Existing Avifauna (Warsaw, Poland)
Authors: Pawłat-Zawrzykraj Agata , Oglęcki Paweł , Podawca Konrad
Published: September 2021

There is no doubt that the NATURA 2000 network has been one of the most relevant tools for nature protection. However, the designated areas within the borders of large cities are subjected to many threats. Traffic noise takes on a very important role in this subject, posing the question of whether NATURA 2000 areas should be located in urban areas strongly affected by noise pollution. This particular topic was exposed and analysed at Bielański Forest NATURA 2000 (PLH140041), located in northern Warsaw, where changes in noise distribution for the years 2007–2017 were examined and described by two types of indicators (Lden and Lnight). The data sources used for the analysis were city road noise maps for 2007, 2012 and 2017. Additionally, sound intensity measurements were taken in two separate groups of hot-spots. The first of these comprised locations determined based on an inventory of avifauna; they represented different habitat types, and were characterized by the highest bird activity. The second group of hot-spots consisted of those designated along roads in order to identify the main sources of traffic noise. The obtained results confirmed the high noise-absorbing ability of the existing vegetation. The avifauna surveys covered 19 forest bird species. Five of them were considered to be the most valuable and rare elements of the local avifauna, whereas 14 appeared to be key species for the functioning of biocenosis and, at the same time, determine the uniqueness of the ecosystem. The study showed that the type of habitat rather than differences in noise levels determines the distribution and abundance of key species. Therefore, there is a necessity to focus on actions that guarantee the maintenance of the existing status in order to counteract habitat deterioration. The investigation confirmed the feasibility of creating these kinds of natural protected areas in large cities, despite their exposure to noise pollution.
Economic Evaluation of Ecological Restoration of Degraded Lands through Protective Afforestation in the South of the Russian Plain
Authors: Korneeva Evgenia A.
Published: September 2021

The latest international climate documents emphasize the great importance of protective forest stands in ensuring the sustainable development of agriculture, and the main requirement is the use of the forest-forming factor by landowners in the interests of improving the environment. In Russia, until recently, the ecological significance of forest plantations was underestimated, which created the ground for discussions about their effectiveness. In this regard, a new approach is proposed that emphasizes the positive impact of forests, including sustainable development, environmental security of the agricultural sector and reducing the degradation of agricultural land. The purpose of the work was an economic assessment and regularities of the dynamics of humus-regulating and nitrogen-phosphorus-potassium (NPK)-regulating efficiency of protective forest plantations on lands with deflation-hazardous soils. By means of a system analysis, the change in the soil cover of land use due to the influence of forest plantations on the balance of soil fertility elements in forested cells is comprehensively analyzed. The different spatial placement of trees from each other under different degrees of deflation in semiarid conditions is modeled. These models are used to determine the nature of the dynamics of soil nutrients in forested areas: in protection zones and outside protection. It is established that the anti-deflationary effect of agroforestry depends on the indicator of the protective forest cover of the land, the level of deflationary danger, and the operational life of the plantings. In semiarid conditions, it increases in proportion to the increase in the protection of land and amounts to EUR 376–EUR 4222 ha−1. With an increase in the intensity of deflation to the level of dust storms, the prevented damage from the loss of soil nutrients increases almost four times. In systems of plantings from early-maturing fast-growing rocks, the anti-deflationary effect is 6–7% higher on an average annual basis than in systems of plantings from long-lasting, slow-growing rocks. The greatest efficiency of forest reclamation in ensuring a positive balance of humus and NPK substances in the soil (EUR 1002–EUR 4222 ha−1) is achieved when placing plantings after 15 H. The study will confirm the need to subsidize the integration of trees into farm systems.
A Note on Stokes Approximations to Leray Solutions of the Incompressible Navier–Stokes Equations in ℝn
Authors: Rigelo Joyce C. , Zingano Janaína P. , Zingano Paulo R.
Published: September 2021

In the early 1980s it was well established that Leray solutions of the unforced Navier–Stokes equations in Rn decay in energy norm for large t. With the works of T. Miyakawa, M. Schonbek and others it is now known that the energy decay rate cannot in general be any faster than t−(n+2)/4 and is typically much slower. In contrast, we show in this note that, given an arbitrary Leray solution u(·,t), the difference of any two Stokes approximations to the Navier–Stokes flow u(·,t) will always decay at least as fast as t−(n+2)/4, no matter how slow the decay of ∥u(·,t)∥L2(Rn) might be.
Effects of Food Types, Frying Frequency, and Frying Temperature on 3-Monochloropropane-1,2-diol Esters and Glycidyl Esters Content in Palm Oil during Frying
Authors: Zhang Jinglin , Zhang Wendi , Zhang Yuanzheng , Huang Mingquan , Sun Baoguo
Published: September 2021

3-Monochloropropanediol esters (3-MCPDE) and glycidyl esters (GE) have high toxicity and have drawn global attention because of their widespread occurrence in refined oils and oil-based foods. In this study, the effects of food type (potato chips and chicken breasts), frying frequency, and frying temperature on the formation of 3-MCPDE and GE in palm oil (PO) were investigated. The results showed that 3-MCPDE was formed easier in chicken breasts than potato chips. The GE content decreased in PO after it was used for frying potato chips and chicken breasts with or without NaCl. Frying frequency was an influencing factor in the formation of 3-MCPDE and the decrease in GE in PO. Frying temperature was positively correlated with GE degradation, while it had a bidirectional effect on the formation of 3-MCPDE. The formation kinetic equations indicated that 3-MCPDE and GE followed zero-order reactions in PO. The estimated activation energy (Ea) of 1,2-bis-palmitoyl-3-chloropropanediol (Pa-Pa, 41.05 kJ/mol) was lower than those of the other three types of 3-MCPDE; this is the first theoretical explanation for why PO contains more 3-MCPD than other edible oils. Among GEs, glycidyl oleate (Li-GE) was degraded more readily than other GEs.
Preparation and Characterization of Ginger Essential Oil Microcapsule Composite Films
Authors: Wang Hua-Hua , Li Meng-Yao , Dong Zhou-Yong , Zhang Tie-Hua , Yu Qing-Yu
Published: September 2021

New food packaging has shown research significance in the face of increasing demand for high-quality foods and growing attention paid to food safety. In this study, ginger essential oil microcapsule composite films were prepared by combining microcapsules prepared by a complex coacervation method with gelatin films, and the mechanical properties and active functions of the composite films were analyzed. Fourier-transform infrared spectroscopy and differential scanning calorimetry confirmed the successful encapsulation of ginger essential oil. The scanning electron microscopy of the composite films showed the microcapsules and gelatin film matrix were highly compatible. During the entire storage period, the antioxidant capacity of the ginger essential oil microcapsule films weakened more slowly than ginger essential oil microcapsules and could be maintained at a relatively high level for a long time. The microcapsule films had excellent inhibitory effects on Escherichia coli, Staphylococcus aureus, and Bacillus subtilis. Therefore, the direct addition of microcapsules to a film matrix can broaden the application range of microcapsules and increase the duration of the release of active ingredients. Ginger essential oil microcapsule films are potential biodegradable food packaging films with long-lasting activity.
Development of Freeze-Thaw Stable Starch through Enzymatic Modification
Authors: Woo Seung-Hye , Kim Ji-Soo , Jeong Hyun-Mo , Shin Yu-Jeong , Hong Jung-Sun , Choi Hee-Don , Shim Jae-Hoon
Published: September 2021

The use of unmodified starch in frozen foods can cause extremely undesirable textural changes after the freeze-thaw process. In this study, using cyclodextrin glucanotransferase (CGTase) and branching enzymes, an amylopectin cluster with high freeze-thaw stability was produced, and was named CBAC. It was found to have a water solubility seven times higher, and a molecular weight 77 times lower, than corn starch. According to the results of a differential scanning calorimetry (DSC) analysis, dough containing 5% CBAC lost 19% less water than a control dough after three freeze-thaw cycles. During storage for 7 days at 4 °C, bread produced using CBAC-treated dough exhibited a 14% smaller retrogradation peak and 37% less hardness than a control dough, suggesting that CBAC could be a potential candidate for clean label starch, providing high-level food stability under repeated freeze-thaw conditions.
Geographical and Varietal Traceability of Chinese Jujubes Based on Physical and Nutritional Characteristics
Authors: Wu Linxia , Li Ling , Zhang Guoguang , Jiang Nan , Ouyang Xihui , Wang Meng
Published: September 2021

Chinese jujube fruits are known for their high nutritional and functional values. To protect advantageous regional jujube fruits, it is important to monitor quality indicators and trace the origin and variety. In this study, 31 quality indicators of Chinese jujubes collected from 6 main producing areas were determined. According to different origins and varieties, Chinese jujube fruits were divided into five and six categories, respectively. To simplify the parameters, eight of the main characteristics, namely, soluble sugar content, fresh mass, edible rate, Na, Mg, K, Zn, and cyclic adenosine monophosphate (cAMP), were screened based on multiple comparison, correlation analysis, and principal component analysis (PCA). According to the eight main parameters, it was found that that both the categorical and cross-validated classification accuracy of linear discriminant analysis (LDA) were 100%. The discrimination accuracy of the testing set samples based on the orthogonal partial least squares-discriminant analysis (OPLS-DA) model were 90 and 93% for geographical and varietal classification, respectively. This indicated that the eight main parameters could be used as the characteristic parameters for the origin and variety traceability of Chinese jujubes.
Effect of the Addition of Different Vegetal Mixtures on the Nutritional, Functional, and Sensorial Properties of Snacks Based on Pseudocereals
Authors: González-Calderón Ana Karen , García-Flores Natalia Alejandra , Elizondo-Rodríguez Ana Sofía , Zavala-López Mariana , García-Lara Silverio , Ponce-García Néstor , Escalante-Aburto Anayansi
Published: September 2021

Quick meals available in markets are popular among consumers. Generally, these products are not recognized as functional foods owing to nutrient-poor composition. In this study, energy snack bars were developed with different formulations, using puffed quinoa, amaranth, cacao liquor, and coconut oil, and the effects of the addition of commercial vegetal mixtures (VM) on nutritional and functional properties were assessed. VM addition showed significant effects on the protein, lipid, and fiber contents, phenolic compounds (PHC) content, and antioxidant activity of the snacks. The control snack showed higher levels of free and bound PHC. The oxygen radical absorbance capacity (ORAC) analyses recorded highest values of free PHC (9392.7 μmol TE/100 g dry weight) in PC65 (concentrate based on a combination of vegetal proteins), whereas the highest bound PHC levels of 47,087 and 46,531 μmol TE/100 g dry weight were observed in PC65 and the control snacks, respectively. Sensorial attributes assessment provided a high score on the hedonic scale, wherein panelists detected no differences among the samples. Altogether, the selection of non-conventional ingredients with high antioxidant activities emerged as a successful strategy to produce sensory acceptable meals.
Probiotic Properties, Prebiotic Fermentability, and GABA-Producing Capacity of Microorganisms Isolated from Mexican Milk Kefir Grains: A Clustering Evaluation for Functional Dairy Food Applications
Authors: Hurtado-Romero Alejandra , Del Toro-Barbosa Mariano , Gradilla-Hernández Misael Sebastián , Garcia-Amezquita Luis Eduardo , García-Cayuela Tomás
Published: September 2021

Isolation and functional characterization of microorganisms are relevant steps for generating starter cultures with functional properties, and more recently, those related to improving mental health. Milk kefir grains have been recently investigated as a source of health-related strains. This study focused on the evaluation of microorganisms from artisanal Mexican milk kefir grains regarding probiotic properties, in vitro fermentability with commercial prebiotics (lactulose, inulin, and citrus pectin), and γ-aminobutyric acid (GABA)-producing capacity. Microorganisms were identified belonging to genera Lactococcus, Lactobacillus, Leuconostoc, and Kluyveromyces. The probiotic properties were assessed by aggregation abilities, antimicrobial activity, antibiotic susceptibility, and resistance to in vitro gastrointestinal digestion, showing a good performance compared with commercial probiotics. Most of isolates maintained a concentration above 6 log colony forming units/mL after the intestinal phase. Specific isolates of Kluyveromyces (BIOTEC009 and BIOTEC010), Leuconostoc (BIOTEC011 and BIOTEC012), and Lactobacillus (BIOTEC014 and BIOTEC15) showed a high fermentability in media supplemented with commercial prebiotics. The capacity to produce GABA was classified as medium for L. lactis BIOTEC006, BIOTEC007, and BIOTEC008; K. lactis BIOTEC009; L. pseudomesenteroides BIOTEC012; and L. kefiri BIOTEC014, and comparable to that obtained for commercial probiotics. Finally, a multivariate approach was performed, allowing the grouping of 2–5 clusters of microorganisms that could be further considered new promising cultures for functional dairy food applications.
Valorization of Polysaccharides Obtained from Dark Tea: Preparation, Physicochemical, Antioxidant, and Hypoglycemic Properties
Authors: Zhu Jiangxiong , Zhou Hui , Zhang Junyao , Li Fanglan , Wei Kang , Wei Xinlin , Wang Yuanfeng
Published: September 2021

The structure and hypoglycemic activity of tea polysaccharides has been extensively studied, while there are few reports on the characterization and hypoglycemic activity of dark tea polysaccharides. The crude dark tea polysaccharide (CDTPS) was optimally extracted from Fuzhuan dark tea. Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed. The results revealed that the compositions, structural characteristics, and biological properties of the six DTPSs were different. Therein, DTPS-4 and DTPS-6 had looser morphology, faster solubility, and a more stable structure. Additionally, DTPS-4 had the optimum in vitro antioxidant capabilities, and DTPS-6 had the strongest in vitro hypoglycemic capabilities. In addition, a correlation analysis revealed that the molecular weight and uronic acid content were significantly related to their antioxidant and hypoglycemic activities. Our results indicated that DTPS-4 and DTPS-6 could be further developed into functional foods or additives, respectively.
Antiviral Properties of Polyphenols from Plants
Authors: Chojnacka Katarzyna , Skrzypczak Dawid , Izydorczyk Grzegorz , Mikula Katarzyna , Szopa Daniel , Witek-Krowiak Anna
Published: September 2021

Polyphenols are active substances against various types of viral infections. Researchers have characterized methods of how to isolate polyphenols without losing their potential to formulate pharmaceutical products. Researchers have also described mechanisms against common viral infections (i.e., influenza, herpes, hepatitis, rotavirus, coronavirus). Particular compounds have been discussed together with the plants in the biomass in which they occur. Quercetin, gallic acid and epigallocatechin are exemplary compounds that inhibit the growth cycle of viruses. Special attention has been paid to identify plants and polyphenols that can be efficient against coronavirus infections. It has been proven that polyphenols present in the diet and in pharmaceuticals protect us from viral infections and, in case of infection, support the healing process by various mechanisms, i.e., they block the entry into the host cells, inhibit the multiplication of the virus, seal blood vessels and protect against superinfection.
A Deep Insight in the Antioxidant Property of Carnosic Acid: From Computational Study to Experimental Analysis
Authors: Wei Jing , Liang Qian , Guo Yuxin , Zhang Weimin , Wu Long
Published: September 2021

Since the deep cause for the anti-oxidation of carnosic acid (CA) against oleic acid (OA) remains unclear, we focused on exploring the CA inhibition mechanism via a combined experimental and computational study. Atomic charge, total molecular energy, phenolic hydroxyl bond dissociation enthalpy (BDE), the highest occupied molecular orbital (HOMO), and the lowest unoccupied orbital (LUMO) energy were first discussed by the B3LYP/6-31G (d,p) level, a density functional method. A one-step hydrogen atom transfer (HAT) was proposed for the anti-oxidation of CA towards OA, and the Rancimat method was carried out for analyzing the thermal oxidation stability. The results indicate that the two phenolic hydroxyl groups located at C7(O15) and C8(O18) of CA exert the highest activity, and the chemical reaction heat is minimal when HAT occurs. Consequently, the activity of C7(O15) (303.27 kJ/mol) is slightly lower than that of C8(O18) (295.63 kJ/mol), while the dissociation enthalpy of phenol hydroxyl groups is much lower than those of α-CH2 bond of OA (C8, 353.92 kJ/mol; C11, 353.72 kJ/mol). Rancimat method and non-isothermal differential scanning calorimetry (DSC) demonstrate that CA outcompetes tertiary butylhydroquinone (TBHQ), a synthetic food grade antioxidant, both in prolonging the oxidation induction period and reducing the reaction rate of OA. The Ea (apparent activation energies of reaction) of OA, TBHQ + OA, and CA + OA were 50.59, 57.32 and 66.29 kJ/mol, revealing that CA could improve the Ea and thermal oxidation stability of OA.
Identification of the Causal Agent of Aqueous Spot Disease of Sweet Cherries (Prunus avium L.) from the Jerte Valley (Cáceres, Spain)
Authors: Serradilla Manuel Joaquín , Moraga Carlos , Ruiz-Moyano Santiago , Tejero Paula , Córdoba María de Guía , Martín Alberto , Hernández Alejandro
Published: September 2021

The pre and postharvest disease named ‘aqueous spot’ is an emerging risk for sweet cherries growing in Jerte Valley (Cáceres, Spain). Early stages of the disease appear in the tree, but it is usually detected after harvesting, during the postharvest period. Symptoms include the appearance of skin discolouration and translucency in the shoulder areas. At the most advanced stages, a mycelium of white colour partially or completely covers the fruit. This manuscript provides a detailed description of the microbes involved in this disease, such as bacteria, yeasts, and moulds. Microbes of different cherry cultivars were studied during two consecutive seasons (2019 and 2020). The counts of bacteria and yeast in damaged tissues were higher (7.05 and 6.38 log10 CFU/g for total aerobic mesophilic microbes and yeasts, respectively) than sound tissues (6.08 and 5.19 log10 CFU/g, respectively). The Enterobacterales order dominated the bacteria population. Among yeasts, Yarrowia lipolytica, in 2019, and Metschnikowia pulcherrima and Metschnikowia viticola, in 2020, were consistently isolated from all samples. The presence of moulds was inconsistently detected at the early stage of this disease by plate counts. However, microscopic observations revealed the presence of hyphae in cherry flesh. Different pathogenic moulds were identified, although white mycelium, identified as Botrytis cinerea by molecular methods, was consistently isolated at later stages. Inoculation tests confirmed the involvement of white-mycelium B. cinerea in the development of this new postharvest disease in the Jerte Valley. Its combination with Enterobacterales enhanced the evolution of rotting, whereas the combination with yeasts decreased and delayed the symptoms. This work presents the first report of a consortia of microorganisms implicated in the development of ‘aqueous spot’, an emerging disease in sweet cherry cultivars in the Jerte Valley.
Natural Antimicrobials as Additives for Edible Food Packaging Applications: A Review
Authors: Punia Bangar Sneh , Chaudhary Vandana , Thakur Neha , Kajla Priyanka , Kumar Manoj , Trif Monica
Published: September 2021

Edible packaging is a swiftly emerging art of science in which edible biopolymers like lipids, polysaccharides, proteins, resins, etc., and other consumable constituents extracted from various non-conventional sources are used alone or imbibed together. Edible packaging with antimicrobial components had led to the development of the hypothesis of active packaging which safeguards the quality of foods as well as health of consumers. Natural antimicrobial agents (NAMAs) like essential oils from spices, bioactive compounds derived from vegetables and fruits, animal and microorganism derived compounds having antimicrobial properties can be potentially used in edible films as superior replcement for synthetic compounds, thus serving the purpose of quality and heath. Most of the natural antimicrobial agents enjoy GRAS status and are safer than their synthetic counterparts. This review focuses on updated literature on the sources, properties and potential applications of NAMAs in the food industry. This review also analyzes the biodegradability and biocompatibility and edibility properties of NAMAs enriched films and it can be concluded that NAMAs are better substitutes but affect the organoleptic as well as the mechanical properties of the films. Despite many advantages, the inclusion of NAMAs into the films needs to be investigated more to quantify the inhibitory concentration without affecting the properties of films and exerting potential antimicrobial action to ensure food safety.
Spices and Seasoning Mixes in European Union—Innovations and Ensuring Safety
Authors: Śmiechowska Maria , Newerli-Guz Joanna , Skotnicka Magdalena
Published: September 2021

Spices are an important group of food products of great importance in nutrition and food technology. They are mainly used to shape the sensory properties of food in gastronomy, in home cooking, and in industry. Ensuring quality and safety is one of the basic tasks of spice producers. The aim of this review is to present the threats to the consumer related to the presence of spices and seasoning mixes in the diet. Therefore, special attention was paid to such risks as excess sodium chloride (and sodium) in spice mixtures, the use of additives influencing the sensory experience, and irregularities in the labeling of spices and seasoning mixes for the presence of additives and allergens. The threats regarding microbiological safety and the presence of heavy metals, pesticides, plant protection products, as well as synthetic fertilizers and undeclared additives are also presented and the issue of adulteration and lack of authenticity of spices and spice mixtures is discussed. Using data from IJHARS planned inspections and notifications registered in the EU Rapid Alert System for Food and Feed (RASFF) for 2015–2019, as well as the results of own research, an analysis of the risks caused by herbs and spices was carried out. Strategic activities of companies producing spices focus, among others, on improving production and expanding the commercial offer with new, attractive products. The article reviews product and process innovations in spice mixes and the methods of ensuring safety in this group of food products.
Differential Diagnosis between Marfan Syndrome and Loeys–Dietz Syndrome Type 4: A Novel Chromosomal Deletion Covering TGFB2
Authors: Nistri Stefano , De Cario Rosina , Sticchi Elena , Spaziani Gaia , Della Monica Matteo , Giglio Sabrina , Favilli Silvia , Giusti Betti , Stefano Pierluigi , Pepe Guglielmina
Published: September 2021

Marfan syndrome (MFS) and Loeys–Dietz syndrome type 4 (LDS4) are two hereditary connective tissue disorders. MFS displays ectopia lentis as a distinguishing, characterising feature, and thoracic aortic ectasia, aneurysm, dissection, and systemic features as manifestations overlapping with LDS4. LDS4 is characterised by the presence of hypertelorism, cleft palate and/or bifid uvula, with possible ectasia or aneurysms in other arteries. The variable age of onset of clinical manifestations makes clinical diagnosis more difficult. In this study, we report the case of a patient with Marfan syndrome diagnosed at our centre at the age of 33 on the basis of typical clinical manifestations of this syndrome. At the age of 38, the appearance of ectasia of the left common iliac artery and tortuosity of the iliac arteries suggested the presence of LDS4. Next Generation Sequencing (NGS) analysis, followed by Array-CGH, allowed the detection of a novel chromosomal deletion including the entire TGFB2 gene, confirming not only the clinical suspicion of LDS4, but also the clinical phenotype associated with the haploinsufficiency mechanism, which is, in turn, associated with the deletion of the entire gene. The same mutation was detected in the two young sons. This emblematic case confirms that we must be very careful in the differential diagnosis of these two pathologies, especially before the age of 40, and that, in young subjects suspected to be affected by MFS in particular, we must verify the diagnosis, extending genetic analysis, when necessary, to the search for chromosomal alterations. Recently, ectopia lentis has been reported in a patient with LDS4, confirming the tight overlap between the two syndromes. An accurate revision of the clinical parameters both characterising and overlapping the two pathologies is highly desirable.
Tracing Key Molecular Regulators of Lipid Biosynthesis in Tuber Development of Cyperus esculentus Using Transcriptomics and Lipidomics Profiling
Authors: Wang Liyan , Jing Meiling , Ahmad Naveed , Wang Yifei , Wang Yijin , Li Jia , Li Xiaowei , Liu Weican , Wang Nan , Wang Fawei , Dong Yuanyuan , Li Haiyan
Published: September 2021

Cyperus esculentus is widely representing one of the important oil crops around the world, which provides valuable resources of edible tubers called tiger nut. The chemical composition and high ability to produce fats emphasize the role of tiger nut in promoting oil crop productivity. However, the underlying molecular mechanism of the production and accumulation of lipids in tiger nut development still remains unclear. Here, we conducted comprehensive transcriptomics and lipidomics analyses at different developmental stages of tuber in Cyperus esculentus. Lipidomic analyses confirmed that the accumulation of lipids including glycolipids, phospholipids, and glycerides were significantly enriched during tuber development from early to mature stage. The proportion of phosphatidylcholines (PC) declined during all stages and phosphatidyl ethanolamine (PE) was significantly declined in early and middle stages. These findings implied that PC is actively involved in triacylglycerol (TAG) biosynthesis during the tubers development, whereas PE may participate in TAG metabolism during early and middle stages. Comparative transcriptomics analyses indicated several genomic and metabolic pathways associated with lipid metabolism during tuber development in tiger nut. The Pearson correlation analysis showed that TAG synthesis in different developmental stages was attributed to 37 candidate transcripts including CePAH1. The up-regulation of diacylglycerol (DAG) and oil content in yeast, resulted from the inducible expression of exogenous CePAH1 confirmed the central role of this candidate gene in lipid metabolism. Our results demonstrated the foundation of an integrative metabolic model for understanding the molecular mechanism of tuber development in tiger nut, in which lipid biosynthesis plays a central role.
Exploring Semi-Quantitative Metagenomic Studies Using Oxford Nanopore Sequencing: A Computational and Experimental Protocol
Authors: Alili Rohia , Belda Eugeni , Le Phuong , Wirth Thierry , Zucker Jean-Daniel , Prifti Edi , Clément Karine
Published: September 2021

The gut microbiome plays a major role in chronic diseases, of which several are characterized by an altered composition and diversity of bacterial communities. Large-scale sequencing projects allowed for characterizing the perturbations of these communities. However, translating these discoveries into clinical applications remains a challenge. To facilitate routine implementation of microbiome profiling in clinical settings, portable, real-time, and low-cost sequencing technologies are needed. Here, we propose a computational and experimental protocol for whole-genome semi-quantitative metagenomic studies of human gut microbiome with Oxford Nanopore sequencing technology (ONT) that could be applied to other microbial ecosystems. We developed a bioinformatics protocol to analyze ONT sequences taxonomically and functionally and optimized preanalytic protocols, including stool collection and DNA extraction methods to maximize read length. This is a critical parameter for the sequence alignment and classification. Our protocol was evaluated using simulations of metagenomic communities, which reflect naturally occurring compositional variations. Next, we validated both protocols using stool samples from a bariatric surgery cohort, sequenced with ONT, Illumina, and SOLiD technologies. Results revealed similar diversity and microbial composition profiles. This protocol can be implemented in a clinical or research setting, bringing rapid personalized whole-genome profiling of target microbiome species.
GWAS Meta-Analysis Reveals Shared Genes and Biological Pathways between Major Depressive Disorder and Insomnia
Authors: Lin Yi-Sian , Wang Chia-Chun , Chen Cho-Yi
Published: September 2021

Major depressive disorder (MDD) is one of the most prevalent and disabling mental disorders worldwide. Among the symptoms of MDD, sleep disturbance such as insomnia is prominent, and the first reason patients may seek professional help. However, the underlying pathophysiology of this comorbidity is still elusive. Recently, genome-wide association studies (GWAS) have begun to unveil the genetic background of several psychiatric disorders, including MDD and insomnia. Identifying the shared genomic risk loci between comorbid psychiatric disorders could be a valuable strategy to understanding their comorbidity. This study seeks to identify the shared genes and biological pathways between MDD and insomnia based on their shared genetic variants. First, we performed a meta-analysis based on the GWAS summary statistics of MDD and insomnia obtained from Psychiatric Genomics Consortium and UK Biobank, respectively. Next, we associated shared genetic variants to genes using two gene mapping strategies: (a) positional mapping based on genomic proximity and (b) expression quantitative trait loci (eQTL) mapping based on gene expression linkage across multiple tissues. As a result, a total of 719 shared genes were identified. Over half (51%) of them are protein-coding genes. Functional enrichment analysis shows that the most enriched biological pathways are related to epigenetic modification, sensory perception, and immunologic signatures. We also identified druggable targets using a network approach. Together, these results may provide insights into understanding the genetic predisposition and underlying biological pathways of comorbid MDD and insomnia symptoms.
Two Decades after Mandibuloacral Dysplasia Discovery: Additional Cases and Comprehensive View of Disease Characteristics
Authors: Jéru Isabelle , Nabil Amira , El-Makkawy Gehad , Lascols Olivier , Vigouroux Corinne , Abdalla Ebtesam
Published: September 2021

Pathogenic variants in the LMNA gene cause a group of heterogeneous genetic disorders, called laminopathies. In particular, homozygous or compound heterozygous variants in LMNA have been associated with “mandibuloacral dysplasia type A” (MADA), an autosomal recessive disorder, characterized by mandibular hypoplasia, growth retardation mainly postnatal, pigmentary skin changes, progressive osteolysis of the distal phalanges and/or clavicles, and partial lipodystrophy. The detailed characteristics of this multisystemic disease have yet to be specified due to its rarity and the limited number of cases described. Here, we report three unrelated Egyptian patients with variable severity of MAD features. Next-generation sequencing using a gene panel revealed a homozygous c.1580G>A-p.Arg527His missense variant in LMNA exon 9 in an affected individual with a typical MADA phenotype. Another homozygous c.1580G>T-p.Arg527Leu variant affecting the same amino acid was identified in two additional patients, who both presented with severe manifestations very early in life. We combined our observations together with data from all MADA cases reported in the literature to get a clearer picture of the phenotypic variability in this disease. This work raises the number of reported MADA families, argues for the presence of the founder effect in Egypt, and strengthens genotype–phenotype correlations.
Isorhamnetin Promotes 53BP1 Recruitment through the Enhancement of ATM Phosphorylation and Protects Mice from Radiation Gastrointestinal Syndrome
Authors: Nishiyama Yuichi , Morita Akinori , Tatsuta Shogo , Kanamaru Misaki , Sakaue Masahiro , Ueda Kenta , Shono Manami , Fujita Rie , Wang Bing , Hosoi Yoshio , Aoki Shin , Sugai Takeshi
Published: September 2021

Flavonoids are a subclass of polyphenols which are attractive, due to possessing various physiological activities, including a radioprotective effect. Tumor suppressor p53 is a primary regulator in the radiation response and is involved in the pathogenesis of radiation injuries. In this study, we revealed that isorhamnetin inhibited radiation cell death, and investigated its action mechanism focusing on DNA damage response. Although isorhamnetin moderated p53 activity, it promoted phosphorylation of ataxia telangiectasia mutated (ATM) and enhanced 53BP1 recruitment in irradiated cells. The radioprotective effect of isorhamnetin was not observed in the presence of ATM inhibitor, indicating that its protective effect was dependent on ATM. Furthermore, isorhamnetin-treated mice survived gastrointestinal death caused by a lethal dose of abdominal irradiation. These findings suggested that isorhamnetin enhances the ATM-dependent DNA repair process, which is presumably associated with the suppressive effect against GI syndrome.
Meiosis in Polyploids and Implications for Genetic Mapping: A Review
Authors: Soares Nina Reis , Mollinari Marcelo , Oliveira Gleicy K. , Pereira Guilherme S. , Vieira Maria Lucia Carneiro
Published: September 2021

Plant cytogenetic studies have provided essential knowledge on chromosome behavior during meiosis, contributing to our understanding of this complex process. In this review, we describe in detail the meiotic process in auto- and allopolyploids from the onset of prophase I through pairing, recombination, and bivalent formation, highlighting recent findings on the genetic control and mode of action of specific proteins that lead to diploid-like meiosis behavior in polyploid species. During the meiosis of newly formed polyploids, related chromosomes (homologous in autopolyploids; homologous and homoeologous in allopolyploids) can combine in complex structures called multivalents. These structures occur when multiple chromosomes simultaneously pair, synapse, and recombine. We discuss the effectiveness of crossover frequency in preventing multivalent formation and favoring regular meiosis. Homoeologous recombination in particular can generate new gene (locus) combinations and phenotypes, but it may destabilize the karyotype and lead to aberrant meiotic behavior, reducing fertility. In crop species, understanding the factors that control pairing and recombination has the potential to provide plant breeders with resources to make fuller use of available chromosome variations in number and structure. We focused on wheat and oilseed rape, since there is an abundance of elucidating studies on this subject, including the molecular characterization of the Ph1 (wheat) and PrBn (oilseed rape) loci, which are known to play a crucial role in regulating meiosis. Finally, we exploited the consequences of chromosome pairing and recombination for genetic map construction in polyploids, highlighting two case studies of complex genomes: (i) modern sugarcane, which has a man-made genome harboring two subgenomes with some recombinant chromosomes; and (ii) hexaploid sweet potato, a naturally occurring polyploid. The recent inclusion of allelic dosage information has improved linkage estimation in polyploids, allowing multilocus genetic maps to be constructed.
The Suitability of the Pacific Islands for Harnessing Ocean Thermal Energy and the Feasibility of OTEC Plants for Onshore or Offshore Processing
Authors: Kim Hyeon-Ju , Lee Ho-Saeng , Lim Seung-Taek , Petterson Michael
Published: September 2021

Ocean Thermal Energy Conversion (OTEC) relies on the thermal differences between ocean surface waters and cooler waters at c. 1000 m depth. The highest and most reliable thermal differentials are in the low latitudes, 20° either side of the equator, including the Pacific Islands region. Whilst in theory OTEC can utilize an inexhaustible amount of stored energy within the oceans, in practice the industry remains in its technical infancy, but with an increasing relevance to a post-fossil-fuel, environmentally conscious world. OTEC does not only produce electricity. OTEC-seawater utilization technologies produce high demand ocean minerals, desalination, a range of waters for aquaculture and hydroponics, and have the potential to produce large quantities of green hydrogen. OTEC is a green energy and could revolutionize the energy and economic landscape of Pacific island countries, providing reliable low-C electricity and a basis for a range of industry. This paper analyses the economics of using OTEC in combination with existing and potential future industries of importance to the Pacific (and other oceanic) regions, including tuna fisheries, seabed minerals and green hydrogen. The conclusions of these analyses suggest that OTEC has the potential to minimize carbon emissions, increase efficiencies, and create new high-quality green-technology industries and livelihoods.
U-Th-He Geochronology of Pyrite from Alteration of the Au-Fe-Skarn Novogodnee-Monto Deposit (Polar Urals, Russia)—The Next Step in the Development of a New Approach for Direct Dating of Ore-Forming Processes
Authors: Yakubovich Olga , Vikentyev Ilya , Ivanova Ekaterina , Podolskaya Mary , Sobolev Ivan , Tyukova Eugenia , Kotov Alexander
Published: September 2021

We report on the application of the U-Th-He method for the direct dating of pyrite from the alteration halo of the Novogodnee-Monto Au-Fe-skarn deposit, Polar Urals. The deposit is genetically related to the formation of volcanogenic complexes of the Ural Paleozoic belt. A modification of the original methodology for measuring U, Th and He isotopes in a single grain allowed us to determine a U-Th-He age of 382 ± 8 Ma (2σ) based on six pyrite samples from the altered rocks of the deposit (U mass fraction ~0.2 mg/kg; Th/U ~3.5; 4He specific volume ~10−5 cm3·STP·g−1). This age is consistent with estimates of the age of ore formation and coeval with the end of the period of island arc magmatic activity. Our results indicate that U-Th-He dating for pyrite samples of ~1 mg in weight from the hydrothermal-metasomatic halo of ore bodies is possible, providing a crucial next step in the development of U-Th-He pyrite geochronology.
Managing Multiple Medications and Their Packaging for Older People in Home Care Nursing: An Interview Study
Authors: Carli Lorenzini Giana
Published: September 2021

Home care nurses assist older people in their daily living and well-being, including medication management. Medication management can be challenging for older people with functional constraints and several chronic conditions. This paper presents how home care nurses manage medications and their packaging for older people at their homes. This study followed an explorative qualitative research design, in which semi-structured interviews were conducted with home care nurses in Sweden (n = 14). The study revealed that home care nurses need to coordinate a multitude of interrelated tasks, with documentation being paramount. Regarding medication management, automated systems were preferred, as they reduce medication errors and facilitate dispensing of medications for each patient when compared with analogue systems still in use (i.e., dosing boxes), commonly used by older people. Importantly, the lack of a common journal system for updates on prescribed medication among health care providers and analogue communication still in place creates space for outdated prescription of medications for patients. There are opportunities for further investigation on how technology can help home care nurses in coordinating medication management tasks with other health care providers, and on receiving updates about medication intake by older patients when the nurse is not at their homes.
Cognitive Reserve Characteristics and Occupational Performance Implications in People with Mild Cognitive Impairment
Authors: Mendoza-Holgado Cristina , Lavado-García Jesús , López-Espuela Fidel , Roncero-Martín Raúl , Canal-Macías María Luz , Vera Vicente , Aliaga Ignacio , Rey-Sánchez Purificación , Pedrera-Zamorano Juan Diego , Moran Jose M.
Published: September 2021

The Cognitive Reserve hypothesis suggests that there are individual differences in the ability to cope with the pathologic changes in Alzheimer’s Disease. The proportion of elderly individuals has increased in recent years; this increase emphasizes the importance of early detection of mild cognitive impairment and the promotion of healthy ageing. The purpose of our study is to characterize cognitive reserve and occupational performance implications in people with mild cognitive impairment. 125 patients with mild cognitive impairment were enrolled. The Montreal Cognitive Assessments (MoCA) was used to evaluate cognitive status and the Cognitive Reserve Index Questionnaire (CRIq) as an indicator of cognitive reserve. Higher level of education was associated with higher MoCA scores (r = 0.290, p = 0.001). Positive significant correlations were observed between MoCA and total CRIq (r = 0.385, p < 0.001) as well as its three sub-domains, education (r = 0.231, p = 0.010), working activity (r = 0.237, p = 0.008) and leisure time (r = 0.319, p < 0.001). This study findings provide the importance of considering socio-behavioral factors in cognitive status. This research helps to describe the importance of engaging occupationally along the whole life-course as a potential protective factor in ageing, and includes a perspective of occupational therapy regarding the hypothesis of cognitive reserve.