bluuebunny/crossref_metadata_2025 huggingface dataset:
Escherichia albertii as a Potential Enteropathogen in the Light of Epidemiological and Genomic Studies
Authors: Leszczyńska Katarzyna , Święcicka Izabela , Daniluk Tamara , Lebensztejn Dariusz , Chmielewska-Deptuła Sylwia , Leszczyńska Dorota , Gawor Jan , Kliber Małgorzata
Published: June 2023

Escherichia albertii is a new enteropathogen of humans and animals. The aim of the study was to assess the prevalence and pathogenicity of E. albertii strains isolated in northeastern Poland using epidemiological and genomic studies. In 2015–2018, a total of 1154 fecal samples from children and adults, 497 bird droppings, 212 food samples, 92 water samples, and 500 lactose-negative E. coli strains were tested. A total of 42 E. albertii strains were isolated. The PCR method was suitable for their rapid identification. In total, 33.3% of E. albertii isolates were resistant to one antibiotic, and 16.7% to two. Isolates were sensitive to cefepime, imipenem, levofloxacin, gentamicin, trimethoprim/sulfamethoxazole, and did not produce ESBL β-lactamases. High genetic variability of E. albertii has been demonstrated. In the PFGE method, 90.5% of the strains had distinct pulsotypes. In MLST typing, 85.7% of strains were assigned distinct sequence types (STs), of which 64% were novel ST types. Cytolethal distending toxin (CDT) and Paa toxin genes were found in 100% of E. albertii isolates. Genes encoding toxins, IbeA, CdtB type 2, Tsh and Shiga (Stx2f), were found in 26.2%, 9.7%, 1.7%, and 0.4% of E. albertii isolates, respectively. The chromosome size of the tested strains ranged from 4,573,338 to 5,141,010 bp (average 4,784,003 bp), and at least one plasmid was present in all strains. The study contributes to a more accurate assessment of the genetic diversity of E. albertii and the potential threat it poses to public health.
DNA Damage Response Mechanisms in Model Systems
Authors: LaRocque Jeannine R. , McVey Mitch
Published: June 2023

Cells are constantly assaulted by endogenous and exogenous sources of DNA damage that threaten genome stability [...]
An Institutional Standardised Protocol for the Treatment of Acute Displaced Midshaft Clavicle Fractures (ADMCFs): Conservative or Surgical Management for Active Patients?
Authors: Biz Carlo , Pozzuoli Assunta , Belluzzi Elisa , Scucchiari Davide , Bragazzi Nicola Luigi , Rossin Alessandro , Cerchiaro Mariachiara , Ruggieri Pietro
Published: June 2023

Background and Objectives: The treatment of acute displaced midshaft clavicle fractures (ADMCFs) is still under debate. The aim of this study was to verify the effectiveness of our institutional protocol by comparing the clinical and radiographic outcomes of two groups of patients with ADMCFs treated operatively and non-operatively. Materials and Methods: active patients with a traumatic, isolated non-pathological ADMCF with at least 1-year clinical and radiographic follow up were included. Surgical treatment was performed in the cases where the residual displacement was higher than 140% after the application of a figure-of-eight bandage (F8-B). All other cases were treated conservatively with a F8-B. A total of 134 patients were enrolled and divided into two groups: surgical and conservative groups, with 59 and 75 patients, respectively. Radiological and clinical parameters were evaluated. Results: Good clinical (Constant-Murley Score, the Quick Disability of the Arm, Shoulder and Hand score, and VAS satisfaction) and radiographic outcomes (initial and residual shortening, initial and residual displacement) were obtained for ADMCFs in both groups. Multivariate analysis showed that patients treated conservatively had better clinical outcomes compared to surgically treated patients (p < 0.001). Return to sports was longer in those treated with surgery. Initial shortening was found to impact clinical outcomes as well as initial displacement. None of the patients showed signs of non-union in both groups. Conclusions: Very good mid-term clinical results can be obtained in adult patients with ADMCFs, conservatively or operatively managed, by applying our institutional treatment protocol based on objective radiographic parameters evaluated in the ER.
Self-Compassion, Work Engagement and Job Performance among Intensive Care Nurses during COVID-19 Pandemic: The Mediation Role of Mental Health and the Moderating Role of Gender
Authors: Bahrami Nejad Joneghani Reihaneh , Bahrami Nejad Joneghani Rayehe , Dustmohammadloo Hakimeh , Bouzari Parisa , Ebrahimi Pejman , Fekete-Farkas Mária
Published: June 2023

(1) Background: The COVID-19 pandemic has highlighted attention on the importance of certain variables in predicting job performance. Among these, mental health is one of the main variables affected by this pandemic. It can have an important mediating role in predicting job performance by individual, occupational, and organizational variables, especially in the nursing community. However, there is little information about its mediation role among the predictors of job performance. This cross-sectional study aimed to examine the role of mental health as a mediating factor in the influence of self-compassion and work engagement on ICU nurses’ job performance during the COVID-19 pandemic, as well as the moderating effect of gender on all model relations. A survey of 424 ICU nurses (men 193 and women 231) was undertaken in three Coronavirus hospitals in Isfahan, Iran. (2) Method: Questionnaires were distributed and collected among the statistical sample, and the data from the questionnaires were analyzed using AMOS24 software (version 24). The research model was evaluated in two stages (the main model and the two sub-models in two gender groups). (3) Result: The analysis revealed that work engagement (β = 0.42, p < 0.001), mental health (β = 0.54, p < 0.001) and job performance (β = 0.51, p < 0.001) were discovered to be positively related to self-compassion. Work engagement is positively associated with mental health (β = 0.16, p < 0.01) and job performance (β = 0.21, p < 0.001), and mental health is linked positively to job performance (β = 0.23, p < 0.001). Furthermore, the effects of self-compassion and work engagement on job performance are mediated by mental health. According to the findings, gender moderates the link between self-compassion and work engagement, work engagement and job performance, and self-compassion and job performance. (4) Conclusion: Mental health has a mediating role in the effect of self-compassion and work engagement on ICU nurses’ job performance. Gender also acted as a moderator in some relationships. Males are dominant in all of these relationships as compared to females.
The Multimorbidity and Lifestyle Correlates in Chinese Population Residing in Macau: Findings from a Community-Based Needs Assessment Study
Authors: Yang Qingling , Zhang Quanzhi , Ngai Fei Wan , Wang Shaoling , Zhang Dexing , Gao Yang , Hao Chun , Wang Harry Haoxiang , Nogueira Oi Ching Bernice Lam , Liu Ming , Molasiotis Alex , Loke Alice , Xie Yaojie
Published: June 2023

Multimorbidity has become one of the most pressing public health concerns worldwide. The objectives of this study were to understand the prevalence of multimorbidity and its relationship with lifestyle factors among Chinese adults in Macau, and to investigate the combined contribution of common lifestyle factors in predicting multimorbidity. Data were collected through face-to-face interviews using a self-reported questionnaire on common chronic diseases, lifestyle factors and sociodemographics. BMI, physical activity, drinking status, smoking status and sleep quality were assessed, and a composite lifestyle score (0 to 9 points) was calculated, and the higher the score, the healthier the lifestyle. A total of 1443 participants were included in the analysis, of whom 55.2% were female, 51.8% were middle aged or elderly and 30.5% completed tertiary education or higher. The prevalence of multimorbidity was 10.3%. The combination of hypertension and hyperlipidaemia was the most common (22.2%) multimorbidity among the participants with multimorbidity. After the adjustment of the covariates, it was found that the participants who were overweight (OR: 1.95, 95% CI: 1.18–3.20, p = 0.009) or obese (OR: 3.76, 95% CI: 2.38–5.96, p < 0.001), former drinkers (OR: 2.43, 95% CI: 1.26–4.69, p = 0.008), and those who reported poor sleep quality (OR: 2.25, 95% CI: 1.49–3.40, p < 0.001) had a high risk of developing multimorbidity. A one-unit increase in the lifestyle score was associated with a 0.33-times reduction in the risk of developing multimorbidity (OR: 0.67; 95% CI: 0.59–0.77, p < 0.001). A combination of lifestyle factors can influence a variety of multimorbidity among the Chinese adults in Macau. Thus, comprehensively assessing the combined contribution of several lifestyle factors in predicting multimorbidity is important.
Sun Protection Counseling at the Pharmacy: A German Qualitative Study on Status Quo, Potential Deficits, and Sources of Information
Authors: Diehl Katharina , Battenberg Maike Carola , Jansen Charlotte , Görig Tatiana
Published: June 2023

The sale of sunscreen products is lucrative for pharmacies, and many people buy sunscreen in pharmacies because they expect to receive good advice on sunscreen use and sun protection. However, little is known about the knowledge level of pharmacists and pharmacy technicians in the field of skin cancer prevention. By conducting a qualitative study in Germany, we aimed to explore what pharmacy personnel know about sun protection and the proper use of sunscreens, where they get their information from, and where they see deficits concerning these topics. We found that there is a need for education regarding the terms skin type and sun protection factor, both of which can be helpful tools when advising customers on sun protection. In addition, participants wished for more independent information from health authorities because sunscreen manufacturing companies, which offer product-specific information, are often the only source of information. Overall, it seems reasonable that pharmacy staff should be educated more about sun protection behavior and the proper use of sunscreen to be able to advise their customers correctly. Our findings offer a starting point for strengthening the role of pharmacies in skin cancer prevention. It seems to make sense to develop and offer tailored handouts for customer counseling. Since sunscreen products are perceived as seasonal products, an educational leaflet can help refresh knowledge about the use of sunscreen and the concepts of skin type and sun protection factor in early summer.
Still on UNESCO’s “Tentative List of World Heritage”? Heritage, Tourism, and Stunted Growth in Sarnath (Varanasi), India
Authors: Shinde Kiran , Singh Rana P. B.
Published: June 2023

It is not unusual for a place to wait for years before being inscribed on the World Heritage List, but Sarnath—the place where the Buddha delivered his first sermon—has been on UNESCO’s “tentative list” for close to 25 years. As a sacred place for Buddhist pilgrimages, Sarnath continues to attract thousands of visitors annually and yet, remains under-developed, unlike other pilgrim towns or religious tourism destinations. This paper examines the reasons for the stunted growth of Sarnath. The findings are based on fieldwork conducted in Sarnath in 2019. The analysis of stakeholder interviews suggests several reasons for Sarnath not being able to capitalize on its religious and cultural heritage for tourism-led development. It was found that the protection of the site as an archaeological park by the Archaeological Survey of India (ASI), which has been controlling development surrounding the park as well as prohibiting the performance of any rituals, have been the key endogenous factors that have contributed to the relative under-development of a Buddhist pilgrimage economy in the town. A handful of monasteries are where Buddhist followers stay and perform their pilgrimage rituals, rendering them as enclaves. Private-sector accommodation is also limited to around 15 hotels and guesthouses. Administratively, Sarnath does not have an independent governance structure. It is governed as just one ward (an electoral constituency) by the Municipal Corporation of Varanasi and is, thus, always under the shadow of Varanasi city. Varanasi is one of the most significant Hindu sacred cities and, hence, visitors stay there for much longer durations to soak in cultural offerings, including religious ceremonies around the Ganga River; a visit to Sarnath is secondary and often limited to a half-day tour. Moreover, the archaeological park at Sarnath serves a recreational purpose as a picnic site for domestic visitors. The exogenous factors mean that the interests of Sarnath (as a Buddhist site) are hardly acknowledged by the Hindu city of Varanasi. This paper argues that the multi-layered contestations that exist at the site level, the town–ward level, and between visitors and managers have further contributed to the poor state of heritage and tourism in Sarnath.
Anaplasma phagocytophilum Encephalitis: A Case Report and Literature Review of Neurologic Manifestations of Anaplasmosis
Authors: Cosiquien Ronin Joshua S. , Stojiljkovic Nenad , Nordstrom Charles W. , Amadi Emeka , Lutwick Larry , Dumic Igor
Published: June 2023

Anaplasma phagocytophilum is an obligate intracellular, Gram-negative pathogen, causative agent of Human Granulocytic Anaplasmosis (HGA). HGA usually manifests as a non-specific febrile illness, accompanied by evidence of leucopenia, thrombocytopenia, and an alteration in liver enzymes. Neurologic manifestations of anaplasmosis are rare and rarely reported. We describe a 62-year-old man who developed encephalitis due to an Anaplasma phagocytophilum infection. The patient favorably responded to intravenous doxycycline and recovered without neurological sequela. In the tick endemic area, clinicians should have a high index of suspicion for tick-borne diseases in patients presenting with neurological deficits. A prompt diagnosis and treatment lead to improvements in morbidity and mortality.
Urinary MicroRNAs as Biomarkers of Urological Cancers: A Systematic Review
Authors: Aveta Achille , Cilio Simone , Contieri Roberto , Spena Gianluca , Napolitano Luigi , Manfredi Celeste , Franco Antonio , Crocerossa Fabio , Cerrato Clara , Ferro Matteo , Del Giudice Francesco , Verze Paolo , Lasorsa Francesco , Salonia Andrea , Nair Rajesh , Walz Jochen , Lucarelli Giuseppe , Pandolfo Savio Domenico
Published: June 2023

MicroRNAs (miRNAs) are emerging as biomarkers for the detection and prognosis of cancers due to their inherent stability and resilience. To summarize the evidence regarding the role of urinary miRNAs (umiRNAs) in the detection, prognosis, and therapy of genitourinary cancers, we performed a systematic review of the most important scientific databases using the following keywords: (urinary miRNA) AND (prostate cancer); (urinary miRNA) AND (bladder cancer); (urinary miRNA) AND (renal cancer); (urinary miRNA) AND (testicular cancer); (urinary miRNA) AND (urothelial cancer). Of all, 1364 articles were screened. Only original studies in the English language on human specimens were considered for inclusion in our systematic review. Thus, a convenient sample of 60 original articles was identified. UmiRNAs are up- or downregulated in prostate cancer and may serve as potential non-invasive molecular biomarkers. Several umiRNAs have been identified as diagnostic biomarkers of urothelial carcinoma and bladder cancer (BC), allowing us to discriminate malignant from nonmalignant forms of hematuria. UmiRNAs could serve as therapeutic targets or recurrence markers of non-muscle-invasive BC and could predict the aggressivity and prognosis of muscle-invasive BC. In renal cell carcinoma, miRNAs have been identified as predictors of tumor detection, aggressiveness, and progression to metastasis. UmiRNAs could play an important role in the diagnosis, prognosis, and therapy of urological cancers.
Vasodilating Effects of Antispasmodic Agents and Their Cytotoxicity in Vascular Smooth Muscle Cells and Endothelial Cells—Potential Application in Microsurgery
Authors: Ueda Misato , Hirayama Yasuki , Ogawa Haruo , Nomura Tadashi , Terashi Hiroto , Sakakibara Shunsuke
Published: June 2023

This study aimed to elucidate the vasodilatory effects and cytotoxicity of various vasodilators used as antispasmodic agents during microsurgical anastomosis. Rat smooth muscle cells (RSMCs) and human coronary artery endothelial cells (HCAECs) were used to investigate the physiological concentrations and cytotoxicity of various vasodilators (lidocaine, papaverine, nitroglycerin, phentolamine, and orciprenaline). Using a wire myograph system, we determined the vasodilatory effects of each drug in rat abdominal aortic sections at the concentration resulting in maximal vasodilation as well as at the surrounding concentrations 10 min after administration. Maximal vasodilation effect 10 min after administration was achieved at the following concentrations: lidocaine, 35 mM; papaverine, 0.18 mM; nitroglycerin, 0.022 mM; phentolamine, 0.11 mM; olprinone, 0.004 mM. The IC50 for lidocaine, papaverine, and nitroglycerin was measured in rat abdominal aortic sections, as well as in RSMCs after 30 min and in HCAECs after 10 min. Phentolamine and olprinone showed no cytotoxicity towards RSMCs or HCAECs. The concentrations of the various drugs required to achieve vasodilation were lower than the reported clinical concentrations. Lidocaine, papaverine, and nitroglycerin showed cytotoxicity, even at lower concentrations than those reported clinically. Phentolamine and olprinone show antispasmodic effects without cytotoxicity, making them useful candidates for local administration as antispasmodics.
Combinations of Photodynamic Therapy with Other Minimally Invasive Therapeutic Technologies against Cancer and Microbial Infections
Authors: Songca Sandile Phinda
Published: June 2023

The rapid rise in research and development following the discovery of photodynamic therapy to establish novel photosensitizers and overcome the limitations of the technology soon after its clinical translation has given rise to a few significant milestones. These include several novel generations of photosensitizers, the widening of the scope of applications, leveraging of the offerings of nanotechnology for greater efficacy, selectivity for the disease over host tissue and cells, the advent of combination therapies with other similarly minimally invasive therapeutic technologies, the use of stimulus-responsive delivery and disease targeting, and greater penetration depth of the activation energy. Brought together, all these milestones have contributed to the significant enhancement of what is still arguably a novel technology. Yet the major applications of photodynamic therapy still remain firmly located in neoplasms, from where most of the new innovations appear to launch to other areas, such as microbial, fungal, viral, acne, wet age-related macular degeneration, atherosclerosis, psoriasis, environmental sanitization, pest control, and dermatology. Three main value propositions of combinations of photodynamic therapy include the synergistic and additive enhancement of efficacy, the relatively low emergence of resistance and its rapid development as a targeted and high-precision therapy. Combinations with established methods such as chemotherapy and radiotherapy and demonstrated applications in mop-up surgery promise to enhance these top three clinical tools. From published in vitro and preclinical studies, clinical trials and applications, and postclinical case studies, seven combinations with photodynamic therapy have become prominent research interests because they are potentially easily applied, showing enhanced efficacy, and are rapidly translating to the clinic. These include combinations with chemotherapy, photothermal therapy, magnetic hyperthermia, cold plasma therapy, sonodynamic therapy, immunotherapy, and radiotherapy. Photochemical internalization is a critical mechanism for some combinations.
Data Normalization of Urine miRNA Profiling from Head and Neck Cancer Patients Treated with Cisplatin
Authors: Torso Nadine de Godoy , Quintanilha Julia Coelho França , Cursino Maria Aparecida , Pincinato Eder de Carvalho , Lima Carmen Silvia Passos , Moriel Patricia
Published: June 2023

The microRNA (miRNA) expression profile by qRT-PCR depends directly on the most appropriate normalization strategy adopted; however, currently there is no universally adequate reference gene. Therefore, this study aimed to determine, considering RNA-Seq results, the most adequate endogenous normalizer for use in the relative quantification of urine miRNAs from head and neck cancer patients, treated with cisplatin chemoradiotherapy. The massive sequencing was performed to identify the miRNAs differentially expressed between the group with cisplatin nephrotoxicity (n = 6) and the one without (n = 6). The candidate endogen normalizer was chosen according to four criteria: (1) the miRNA must be expressed in most samples; (2) the miRNA must have a fold change value between 0.99 and 1.01; (3) the miRNA must have a p-value ≥ 0.98; and (4) the miRNA must not be commented on by the final GeneGlobe (Qiagen, Hilden, Germany) analysis. Four miRNAs met all the criteria (hsa-miR-363-5p, hsa-miR-875-5p, hsa-miR-4302, and hsa-miR-6749-5p) and were selected for validation by qRT-PCR in a cohort of 49 patients (including the 12 sequencing participants). Only hsa-miR-875-5p was shown to be an adequate normalizer for the experimental condition under investigation, as it exhibited invariant expression between the two groups.
Double- or Triple-Tiered Protection: Prospects for the Sustainable Application of Copper-Based Antimicrobial Compounds for Another Fourteen Decades
Authors: Yu Yue , Liu Haifeng , Xia Haoran , Chu Zhaohui
Published: June 2023

Copper (Cu)-based antimicrobial compounds (CBACs) have been widely used to control phytopathogens for nearly fourteen decades. Since the first commercialized Bordeaux mixture was introduced, CBACs have been gradually developed from highly to slightly soluble reagents and from inorganic to synthetic organic, with nanomaterials being a recent development. Traditionally, slightly soluble CBACs form a physical film on the surface of plant tissues, separating the micro-organisms from the host, then release divalent or monovalent copper ions (Cu2+ or Cu+) to construct a secondary layer of protection which inhibits the growth of pathogens. Recent progress has demonstrated that the release of a low concentration of Cu2+ may elicit immune responses in plants. This supports a triple-tiered protection role of CBACs: break contact, inhibit microorganisms, and stimulate host immunity. This spatial defense system, which is integrated both inside and outside the plant cell, provides long-lasting and broad-spectrum protection, even against emergent copper-resistant strains. Here, we review recent findings and highlight the perspectives underlying mitigation strategies for the sustainable utilization of CBACs.
Resveratrol Protects Rat Ovarian Luteinized Granulosa Cells from H2O2-Induced Dysfunction by Activating Autophagy
Authors: Cai Minghui , Sun Haijuan , Huang Yujia , Yao Haixu , Zhao Chen , Wang Jiao , Zhu Hui
Published: June 2023

Resveratrol performs a variety of biological activities, including the potential regulation of autophagy. However, it is unclear whether resveratrol protects against luteal dysfunction and whether autophagy involves the regulation of resveratrol. This study aims to investigate whether resveratrol can regulate autophagy to resist H2O2-induced luteinized granulosa cell dysfunction in vitro. Our results showed that resveratrol can enhance cell viability, stimulate the secretion of progesterone and estradiol, and resist cell apoptosis in H2O2-induced luteinized granulosa cell dysfunction. Resveratrol can activate autophagy by stimulating the expression of autophagy-related genes at the transcriptional and translational levels and increasing the formation of autophagosomes and autophagolysosomes. Rapamycin, 3-methyladenine, and bafilomycin A1 regulated the levels of autophagy-related genes in H2O2-induced luteinized granulosa cell dysfunction and further confirmed the protective role of autophagy activated by resveratrol. In conclusion, resveratrol activates autophagy to resist H2O2-induced oxidative dysfunction, which is crucial for stabilizing the secretory function of luteinized granulosa cells and inhibiting apoptosis. This study may contribute to revealing the protective effects of resveratrol on resisting luteal dysfunction from the perspective of regulating autophagy.
How Do Microorganisms Influence the Development of Endometriosis? Participation of Genital, Intestinal and Oral Microbiota in Metabolic Regulation and Immunopathogenesis of Endometriosis
Authors: Sobstyl Anna , Chałupnik Aleksandra , Mertowska Paulina , Grywalska Ewelina
Published: June 2023

Microorganisms inhabiting the human body play an extremely key role in its proper functioning, as well as in the development of the immune system, which, by maintaining the immune balance, allows you to enjoy health. Dysbiosis of the intestinal microbiota, or in the oral cavity or reproductive tract, understood as a change in the number and diversity of all microorganisms inhabiting them, may correlate with the development of many diseases, including endometriosis, as researchers have emphasized. Endometriosis is an inflammatory, estrogen-dependent gynecological condition defined by the growth of endometrial cells outside the uterine cavity. Deregulation of immune homeostasis resulting from microbiological disorders may generate chronic inflammation, thus creating an environment conducive to the increased adhesion and angiogenesis involved in the development of endometriosis. In addition, research in recent years has implicated bacterial contamination and immune activation, reduced gastrointestinal function by cytokines, altered estrogen metabolism and signaling, and abnormal progenitor and stem cell homeostasis, in the pathogenesis of endometriosis. The aim of this review was to present the influence of intestinal, oral and genital microbiota dysbiosis in the metabolic regulation and immunopathogenesis of endometriosis.
Genome-Wide Analysis of Exocyst Complex Subunit Exo70 Gene Family in Cucumber
Authors: Liu Liu , Gu Chaoheng , Zhang Jiahao , Guo Jingyu , Zhang Xiaolan , Zhou Zhaoyang
Published: June 2023

Cucumber (Cucumis sativus L.) is an important vegetable worldwide, but its yield is affected by a wide range of pathogens and pests. As the major subunit of the exocyst complex, the roles of Exo70 members have been shown in Arabidopsis and rice, but their function are unknown in cucumber. Here, we identified 18 CsExo70 members in cucumber, which were divided into three groups (Exo70.1–Exo70.3) and nine subgroups (Exo70A–Exo70I) based on the phylogenetic tree. Subsequently, systematical analyses were performed, including collinearity, gene structure, cis-acting elements, conserved motifs, expression patterns, and subcellular localization. Our results showed that CsExo70 genes were generally expressed in all tissues, and CsExo70C1 and CsExo70C2 were highly expressed in the stamen. Moreover, the expression levels of most CsExo70 genes were induced by Pseudomonas syringae pv. lachrymans (Psl) and Fusarium oxysporum f. sp. cucumerinum Owen (Foc), especially CsExo70E2 and CsExo70H3. In addition, these CsExo70s displayed similar location patterns with discrete and punctate signals in the cytoplasm. Together, our results indicate that CsExo70 members may be involved in plant development and resistance, and provide a reference for future in-depth studies of Exo70 genes in cucumber.
Genome-Wide Identification and Expression Analysis of ACTIN Family Genes in the Sweet Potato and Its Two Diploid Relatives
Authors: Xia Shuanghong , Zhang Huan , He Shaozhen
Published: June 2023

ACTINs are structural proteins widely distributed in plants. They are the main components of microfilaments and participate in many crucial physiological activities, including the maintenance of cell shape and cytoplasmic streaming. Meanwhile, ACTIN, as a housekeeping gene, is widely used in qRT-PCR analyses of plants. However, ACTIN family genes have not been explored in the sweet potato. In this study, we identified 30, 39, and 44 ACTINs in the cultivated hexaploid sweet potato (Ipomoea batatas, 2n = 6x = 90) and its two diploid relatives, Ipomoea trifida (2n = 2x = 30) and Ipomoea triloba (2n = 2x = 30), respectively, via analysis of their genome structure and by phylogenetic characterization. These ACTINs were divided into six subgroups according to their phylogenetic relationships with Arabidopsis thaliana. The physiological properties of the protein, chromosome localization, phylogenetic relationship, gene structure, promoter cis-elements, protein interaction networks, and expression patterns of these 113 ACTINs were systematically investigated. The results suggested that homologous ACTINs are differentiated in the sweet potato and its two diploid relatives, and play various vital roles in plant growth, tuberous root development, hormone crosstalk, and abiotic stress responses. Some stable ACTINs that could be used as internal reference genes were found in the sweet potato and its two diploid relatives, e.g., IbACTIN18, -20, and -16.2; ItfACTIN2.2, -16, and -10; ItbACTIN18 and -19.1. This work provides a comprehensive comparison and furthers our understanding of the ACTIN genes in the sweet potato and its two diploid relatives, thereby supplying a theoretical foundation for their functional study and further facilitating the molecular breeding of sweet potatoes.
Brain-Biomarker Changes in Body Fluids of Patients with Parkinson’s Disease
Authors: Cocco Cristina , Manai Antonio Luigi , Manca Elias , Noli Barbara
Published: June 2023

Parkinson’s disease (PD) is an incurable neurodegenerative disease that is rarely diagnosed at an early stage. Although the understanding of PD-related mechanisms has greatly improved over the last decade, the diagnosis of PD is still based on neurological examination through the identification of motor symptoms, including bradykinesia, rigidity, postural instability, and resting tremor. The early phase of PD is characterized by subtle symptoms with a misdiagnosis rate of approximately 16–20%. The difficulty in recognizing early PD has implications for the potential use of novel therapeutic approaches. For this reason, it is important to discover PD brain biomarkers that can indicate early dopaminergic dysfunction through their changes in body fluids, such as saliva, urine, blood, or cerebrospinal fluid (CSF). For the CFS-based test, the invasiveness of sampling is a major limitation, whereas the other body fluids are easier to obtain and could also allow population screening. Following the identification of the crucial role of alpha-synuclein (α-syn) in the pathology of PD, a very large number of studies have summarized its changes in body fluids. However, methodological problems have led to the poor diagnostic/prognostic value of this protein and alternative biomarkers are currently being investigated. The aim of this paper is therefore to summarize studies on protein biomarkers that are alternatives to α-syn, particularly those that change in nigrostriatal areas and in biofluids, with a focus on blood, and, eventually, saliva and urine.
Genome-Wide Analysis of the MADS-box Gene Family and Expression Analysis during Anther Development in Salvia miltiorrhiza
Authors: Chai Songyue , Li Kexin , Deng Xuexue , Wang Long , Jiang Yuanyuan , Liao Jinqiu , Yang Ruiwu , Zhang Li
Published: June 2023

MADS-box genes constitute a large family of transcription factors that play important roles in plant growth and development. However, our understanding of MADS-box genes involved in anther development and male sterility in Salvia miltiorrhiza is still limited. In this study, 63 MADS-box genes were identified from the genome of the male sterility ecotype Sichuan S. miltiorrhiza (S. miltiorrhiza_SC) unevenly distributed among eight chromosomes. Phylogenetic analysis classified them into two types and 17 subfamilies. They contained 1 to 12 exons and 10 conserved motifs. Evolution analysis showed that segmental duplication was the main force for the expansion of the SmMADS gene family, and duplication gene pairs were under purifying selection. Cis-acting elements analysis demonstrated that the promoter of SmMADS genes contain numerous elements associated with plant growth and development, plant hormones, and stress response. RNA-seq showed that the expression levels of B-class and C-class SmMADS genes were highly expressed during anther development, with SmMADS11 likely playing an important role in regulating anther development and male fertility in S. miltiorrhiza_SC. Overall, this study provides a comprehensive analysis of the MADS-box gene family in S. miltiorrhiza, shedding light on their potential role in anther development and male sterility.
Phototherapy of Alzheimer’s Disease: Photostimulation of Brain Lymphatics during Sleep: A Systematic Review
Authors: Semyachkina-Glushkovskaya Oxana , Penzel Thomas , Poluektov Mikhail , Fedosov Ivan , Tzoy Maria , Terskov Andrey , Blokhina Inna , Sidorov Viktor , Kurths Jürgen
Published: June 2023

The global number of people with Alzheimer’s disease (AD) doubles every 5 years. It has been established that unless an effective treatment for AD is found, the incidence of AD will triple by 2060. However, pharmacological therapies for AD have failed to show effectiveness and safety. Therefore, the search for alternative methods for treating AD is an urgent problem in medicine. The lymphatic drainage and removal system of the brain (LDRSB) plays an important role in resistance to the progression of AD. The development of methods for augmentation of the LDRSB functions may contribute to progress in AD therapy. Photobiomodulation (PBM) is considered to be a non-pharmacological and safe approach for AD therapy. Here, we highlight the most recent and relevant studies of PBM for AD. We focus on emerging evidence that indicates the potential benefits of PBM during sleep for modulation of natural activation of the LDRSB at nighttime, providing effective removal of metabolites, including amyloid-β, from the brain, leading to reduced progression of AD. Our review creates a new niche in the therapy of brain diseases during sleep and sheds light on the development of smart sleep technologies for neurodegenerative diseases.
Hybrid Nanocomposite Solid Electrolytes (n-C4H9)4NBF4–MgO
Authors: Mateyshina Yulia , Stebnitskii Ivan , Shivtsov Danil , Ilyina Ekaterina , Ulihin Artem , Bukhtiyarov Andrey , Uvarov Nikolai
Published: June 2023

Hybrid nanocomposite materials Bu4NBF4–MgO were obtained using a nanocrystalline MgO with a specific surface area of 324 m2/g and the grains size of 5.1 nm. As a result of the strong adhesion, the salt transforms into an interface-stabilized amorphous state within the thin layer near the interface. The analysis of the DSC data allowed one to estimate the concentration and the thickness of this amorphous layer as 4.8 nm. The amorphous interface phase has an enhanced ionic conductivity. As a result, conductivity of the nanocomposite increases with the concentration of the amorphous phase and reaches 1.1 × 10−3 S/cm at 150 °C at a concentration of the MgO additive x = 0.90 corresponding to the maximum content of the amorphous phase. The conductivity of the nanocomposite is by three orders of magnitude higher than the conductivity of pure Bu4NBF4. The nanocomposites are electrochemically stable up to 2.5 V. At high concentrations of MgO when the total volume of the salt is small the composites become nano- and mesoporous.
Complete Desorption of Hybrid Nanoclays Composed of Hydrotalcite and Disperse Dye
Authors: López-Rodríguez Daniel , Micó-Vicent Bàrbara , Jordán-Núñez Jorge , Montava-Seguí Ignacio , Bou-Belda Eva
Published: June 2023

Clays are considered great nanoadsorbents for many materials, including textile dyes. The use of these materials for cleaning textile wastewater is well known; however, it is not at all common to find applications for the hybrid materials formed from the clay and dye. In this work, a dye-loaded clay material was used to make new dye baths and colour a polyester textile substrate. The same hybrid could be used several times as it did not use all the adsorbed dye in a single dyeing. The hybrid obtained from hydrotalcite (nanoclay) and the dispersed red 1 dye was analysed by measuring the colour obtained, carrying out an X-ray diffraction analysis that provided information after each desorption–dyeing process, and using infrared spectroscopy to analyse the specific bands of each characteristic group. Both analyses showed that the amount of dye present in the hybrid decreases. Thermogravimetry (TGA), surface area and porosity measurements (BET), and X-ray photoelectron spectroscopy (XPS) tests were conducted. Chemical stability was assessed by subjecting the hybrid to the actions of different reagents. In addition, colour fastness tests were carried out after dyeing and washing the polyester test tubes to check for the correct fixing of the dye to the fibre. These fastness results showed that the dyeing was carried out correctly and as if it was a conventional dyeing process.
A Novel Ex Vivo Model to Study Therapeutic Treatments for Myelin Repair following Ischemic Damage
Authors: Werner Luisa , Gliem Michael , Rychlik Nicole , Pavic Goran , Reiche Laura , Kirchhoff Frank , Silva Oliveira Junior Markley , Gruchot Joel , Meuth Sven G. , Küry Patrick , Göttle Peter
Published: June 2023

Stroke is a major reason for persistent disability due to insufficient treatment strategies beyond reperfusion, leading to oligodendrocyte death and axon demyelination, persistent inflammation and astrogliosis in peri-infarct areas. After injury, oligodendroglial precursor cells (OPCs) have been shown to compensate for myelin loss and prevent axonal loss through the replacement of lost oligodendrocytes, an inefficient process leaving axons chronically demyelinated. Phenotypic screening approaches in demyelinating paradigms revealed substances that promote myelin repair. We established an ex vivo adult organotypic coronal slice culture (OCSC) system to study repair after stroke in a resource-efficient way. Post-photothrombotic OCSCs can be manipulated for 8 d by exposure to pharmacologically active substances testing remyelination activity. OCSCs were isolated from a NG2-CreERT2-td-Tomato knock-in transgenic mouse line to analyze oligodendroglial fate/differentiation and kinetics. Parbendazole boosted differentiation of NG2+ cells and stabilized oligodendroglial fate reflected by altered expression of associated markers PDGFR-α, CC1, BCAS1 and Sox10 and GFAP. In vitro scratch assay and chemical ischemia confirmed the observed effects upon parbendazole treatment. Adult OCSCs represent a fast, reproducible, and quantifiable model to study OPC differentiation competence after stroke. Pharmacological stimulation by means of parbendazole promoted OPC differentiation.
Consideration of the Variable Contact Geometry in Vibratory Roller Compaction
Authors: Pistrol Johannes , Hager Mario , Kopf Fritz , Adam Dietmar
Published: June 2023

Vibratory rollers are mainly used for the near-surface compaction of granular media for a wide variety of construction tasks. In addition to the pronounced depth effect, vibratory rollers have offered the possibility of work-integrated compaction control (intelligent compaction) for decades. State-of-the-art measurement values for intelligent compaction (ICMVs) only take into account, if at all, a constant geometry of the contact area between the drum and soil. Therefore, this paper introduces a comparatively simple mechanical model, which describes the dynamic interaction between the vibrating drum and the underlying soil during compaction to investigate the influence of the changing geometry of the contact area on the motion behavior of the vibrating drum. The model is tested on realistic soil and machine parameters, and the results of the simulation with varying drum contact geometry are compared to a conventional simulation with a fixed contact geometry. The analysis shows that only a consideration of the varying drum contact geometry can map the dynamic interaction between the vibrating drum and soil sufficiently and provide a motion behavior of the drum that is in good accordance with the field measurements.
Green Organoselenium Chemistry: Selective Syntheses of New 1,4-Thiaselenine Derivatives Based on Reactions of Thiaselenole Reagent with Alcohols and Water
Authors: Amosova Svetlana V. , Filippov Andrey S. , Makhaeva Nataliya A. , Albanov Alexander I. , Potapov Vladimir A.
Published: June 2023

Environmentally friendly synthetic methods were developed for the selective preparation of new 2,3-dihydro-1,4-thiaselenine derivatives in high yields based on the reactions of 2-bromomethyl-1,3-thiaselenole with alcohols and water at room temperature. The reaction of 2-bromomethyl-1,3-thiaselenole with alcohols was accompanied by a rearrangement with ring extension, leading to six-membered heterocyclic compounds, a new family of 2-organyloxy-2,3-dihydro-1,4-thiaselenines, in 80–96% yields. The remarkable cascade reactions of 2-bromomethyl-1,3-thiaselenole with water afforded 2,3-dihydro-1,4-thiaselenines functionalized with the (Z)-S-CH=CH-Se fragment and one or two highly reactive aldehyde groups. The latter aldehydes were functionalized by the reactions with alcohols and glycols to give new polyfunctionalized compounds, containing two double bonds, two sulfur atoms, two selenium atoms, and two or four oxygen atoms, in high yields.
A Comprehensive Review of Architecture, Communication, and Cybersecurity in Networked Microgrid Systems
Authors: Aghmadi Ahmed , Hussein Hossam , Polara Ketulkumar Hitesh , Mohammed Osama
Published: June 2023

Networked microgrids (NMGs) are developing as a viable approach for integrating an expanding number of distributed energy resources (DERs) while improving energy system performance. NMGs, as compared to typical power systems, are constructed of many linked microgrids that can function independently or as part of a more extensive network. This allows NMGs to be more flexible, dependable, and efficient. The present study comprehensively investigates architecture, communication, and cybersecurity issues in NMGs. This comprehensive study examines various aspects related to networked microgrids (NMGs). It explores the architecture of NMGs, including control techniques, protection, standards, and the challenges associated with their adoption. Additionally, it investigates communication in NMGs, focusing on the technologies, protocols, and the impact of communication on the functioning of these systems. Furthermore, this study addresses cybersecurity challenges specific to NMGs, such as diverse cyberattack types, detection and mitigation strategies, and the importance of awareness training. The findings of this study offer valuable insights for NMG researchers and practitioners, emphasizing critical aspects that must be considered to ensure the safe and efficient operation of these systems.
Bone Marrow Assessment in Liver Cirrhosis Patients with Otherwise Unexplained Peripheral Blood Cytopenia
Authors: Koschade Sebastian E. , Moser Laura M. , Sokolovskiy Artur , Michael Florian A. , Serve Hubert , Brandts Christian H. , Finkelmeier Fabian , Zeuzem Stefan , Trebicka Jonel , Ferstl Philip , Ballo Olivier
Published: June 2023

We performed a retrospective single-center analysis to investigate the diagnostic yield of bone marrow puncture in patients with liver cirrhosis and cytopenia. Liver cirrhosis patients receiving bone marrow aspiration or biopsy for the diagnostic work-up of otherwise unexplained peripheral blood cytopenia at our institution between 2004 and 2020 were enrolled in this study. We evaluated findings from cytologic, histologic and immunologic assessment and final diagnostic outcomes. A total of 118 patients with a median age of 55 years and a median Child–Pugh score of B (8 points) were enrolled. The main etiologies of liver cirrhosis were viral hepatitis (B and C) or chronic alcohol consumption. The majority of patients (60%) exhibited concurrent anemia, leukocytopenia and thrombocytopenia. Bone marrow assessment revealed normal, unspecific or reactive alterations in 117 out of 118 patients (99%). One patient was diagnosed with myelodysplastic syndrome. Our findings suggest that peripheral blood cytopenia in patients with liver cirrhosis is rarely associated with a primary bone marrow pathology.
Effect of Visual Booklets to Improve Bowel Preparation in Colonoscopy: Systematic Review with Meta-Analysis
Authors: Losurdo Giuseppe , Martino Maria Ludovica , De Bellis Margherita , Celiberto Francesca , Rizzi Salvatore , Principi Mariabeatrice , Ierardi Enzo , Iannone Andrea , Di Leo Alfredo
Published: June 2023

An optimal bowel preparation for colonoscopy is essential to increasing the quality of the examination. Visual booklets have been proposed with conflicting results to enhance bowel preparation. A literature search was performed in March 2023 in the most important databases. Only RCTs were selected. We calculated odd ratios (OR) for dichotomous outcomes. Mean differences (MD) or standardized mean differences (SMD) were used for continuous outcomes. We estimated heterogeneity with the Chi2 and the I2 statistics. In cases of high heterogeneity, a random effect model was used. Six studies were selected, enrolling 1755 patients overall. Adequate bowel preparation was observed in 86.7% of the booklet group versus 77.5% of the control group, with an OR = 2.31 in favor of the booklet. In studies using a 4-L PEG-based preparation, no difference compared to controls was observed, while in non-PEG formulations, preparation with booklets was better than in controls (OR = 5.10, 95% CI 1.82–14.27, p = 0.002). Two studies were performed in an inpatient setting without any differences between booklets and controls, while outpatients receiving booklets had better results (OR = 7.13, 95% CI 5.39–9.45, p < 0.001). The adenoma detection rate was similar between the two groups. In conclusion, booklets are useful to improve bowel preparation. Outpatient settings and preparations not containing PEG could benefit more from booklets.
Current Insights into the Potential Role of fMRI in Discovering the Mechanisms Underlying Obesity
Authors: Szmygin Hanna , Szmygin Maciej , Cheda Mateusz , Kłobuszewski Bartosz , Drelich-Zbroja Anna , Matyjaszek-Matuszek Beata
Published: June 2023

Obesity is becoming one of the major global health concerns. This chronic disease affects around 650 million people worldwide and is an underlying cause of a number of significant comorbidities. According to the World Health Organization (WHO) report on obesity from 2022, this disorder became the fourth leading cause of deaths in Europe. Thus, understanding the mechanisms underlying obesity is of essential importance to successfully prevent and treat this disease. The aim of this study was to review the current insights into the potential role of fMRI in discovering the mechanisms underlying obesity on the basis of recent scientific literature published up to December 2022 and searches of the PubMed, Google Scholar and Web of Science databases. The literature assessed indicated that a growing body of evidence suggests that obesity leads to changes in both structure and connectivity within the central nervous system. Emerging data from recent functional magnetic resonance imaging (fMRI) studies prove that obese individuals present an increased motivational drive to eat as well as impaired processing in reward- and control-related brain regions. Apart from this, it is clear that fMRI might be a useful tool in detection of obesity-induced changes within the central nervous system.