bluuebunny/crossref_metadata_2025 huggingface dataset:
Physico-Chemical Characteristics of Spodumene Concentrate and Its Thermal Transformations
Authors: Fosu Allen Yushark , Kanari Ndue , Bartier Danièle , Hodge Harrison , Vaughan James , Chagnes Alexandre
Published: December 2021

Spodumene concentrate from the Pilbara region in Western Australia was characterized by X-ray diffraction (XRD), Scanning Electron Microscope Energy Dispersive Spectroscopy (SEM-EDS) and Mineral Liberation Analysis (MLA) to identify and quantify major minerals in the concentrate. Particle diameters ranged from 10 to 200 microns and the degree of liberation of major minerals was found to be more than 90%. The thermal behavior of spodumene and the concentration of its polymorphs were studied by heat treatments in the range of 900 to 1050 °C. All three polymorphs of the mineral (α, γ and β) were identified. Full transformation of the α-phase was achieved at 975 °C and 1000 °C after 240 and 60 min treatments, respectively. SEM images of thermally treated concentrate revealed fracturing of spodumene grains, producing minor cracks initially which became more prominent with increasing temperature. Material disintegration, melting and agglomeration with gangue minerals were also observed at higher temperatures. The metastable γ-phase achieved a peak concentration of 23% after 120 min at 975 °C. We suggest 1050 °C to be the threshold temperature for the process where even a short residence time causes appreciable transformation, however, 1000 °C may be the ideal temperature for processing the concentrate due to the degree of material disintegration and α-phase transformation observed. The application of a first-order kinetic model yields kinetic parameters which fit the experimental data well. The resultant apparent activation energies of 655 and 731 kJ mol−1 obtained for α- and γ-decay, respectively, confirm the strong temperature dependence for the spodumene polymorph transformations.
Temperature Dependency on the Microscopic Mechanism in the Normal Direction of Wrought AZ31 Sheet under Dynamic Compressive Behavior
Authors: Zhang Feng , Sun Mingcheng , Sun Baojie , Zhang Fengzheng , Bai Yikui , Liu Zheng
Published: December 2021

In order to analyze the competitive relationship of different deformation mechanisms in wrought AZ31 magnesium alloy, the dynamic compressive experiments were conducted by a Split Hopkinson Pressure Bar (SHPB) apparatus and a resistance-heated furnace in the range of temperature between 20 and 350 °C at the strain rate of 1000 s−1. With the help of Electron Backscattered Diffraction (EBSD) observation, theoretical calculated Schmid Factor (SF), Critical Resolved Shear Stress (CRSS), and critical equivalent stress (σ0.2), the dynamic compressive deformation behavior and corresponding mechanism of wrought AZ31 magnesium alloy along the normal direction (ND) were revealed in the current study. The results demonstrate that the c-axis of grains are gradually reoriented parallel to the normal direction of wrought AZ31-ND sheet with the temperature increasing, except the dynamic recrystallization (DRX) mechanism was activated or grains grew up. The non-basal slip and 101¯2 tension twinning are respectively the predominant deformation mechanisms at lower temperatures (≤250 °C) and higher temperatures (≥250 °C). The predominant type of DRX mechanism of wrought AZ31-ND sheet is rotational dynamic recrystallization (RDRX), which is regarded as an obstacle for the kernel misorientation concentration region enhancement.
Bond Tests on Clay Bricks and Natural Stone Masonry Externally Bonded with FRP
Authors: Leone Marianovella , Aiello Maria Antonietta
Published: December 2021

Nowadays, the solution of durability problems of existing buildings has a key role in civil engineering, in which there is an ever-increasing need for building restorations. Over the past 50 years, there is a growing interest in a new composite material, fibre-reinforced polymer (FRP), suitable for increasing the resistance and the stability of existing buildings and, consequently, for extending their service life. In this context, the effectiveness of the strengthening system is related to the bond behaviour that is influenced by several parameters such as bond length, the stiffness of the reinforcement, the mechanical properties of the substrate, environmental conditions, etc. This paper aims to analyse the main experimental results from shear tests performed on two kinds of masonry substrates and different types of FRP reinforcements. The purpose is to highlight the role played by many parameters to the bond behaviour of these systems: the mechanical properties of substrates; the stiffness of reinforcements; the type of supports (i.e., unit or masonry unit). The obtained experimental results underlined that the specimens realised with masonry unit show an increase in debonding load and different stress transfer mechanisms along the bonded length with respect to the specimens with a unit substrate. The analysis of the data revealed that the presence of mortar joints cannot be neglected because it influences the interface global performance.
Characterization of Cobalt-Based Stellite 6 Alloy Coating Fabricated by Laser-Engineered Net Shaping (LENS)
Authors: Durejko Tomasz , Łazińska Magdalena
Published: December 2021

The results of microstructure and mechanical properties evaluation of a Stellite 6 (Co-6) alloy deposited on X22CrMoV12-1 substrate by the laser-engineered net shaping (LENSTM) technology are presented in this paper. The Stellite 6 alloy is widely used in industry due to its excellent wear resistance at elevated temperatures and corrosive environments. Specific properties of this alloy are useful in many applications, e.g., as protective coatings in steam turbine components. In this area, the main problems are related to the fabrication of coatings on complex-shaped parts, the low metallurgical quality of obtained coatings, and its insufficient adhesion to a substrate. The results of recently performed investigations proved that the LENS technology is one of the most effective manufacturing techniques of the Co-6 alloy coatings (especially deposited on complex-shaped turbine parts). The microstructural and phase analyses of obtained Stellite 6 coatings were carried out by light microscopy techniques and X-ray diffraction analysis. A chemical homogeneity of Co-6 based layers and a fluctuation of chemical composition in coating–substrate zone after the laser deposition were analyzed using an energy dispersive X-ray spectrometer coupled with scanning electron microscopy. The room temperature strength and ductility of the LENS processed layers were determined in static bending tests.
Effects of Heat and Momentum Gain Differentiation during Gas Detonation Spraying of FeAl Powder Particles into the Water
Authors: Senderowski Cezary , Panas Andrzej J. , Fikus Bartosz , Zasada Dariusz , Kopec Mateusz , Korytchenko Kostyantyn V.
Published: December 2021

In this paper, dynamic interactions between the FeAl particles and the gaseous detonation stream during supersonic D-gun spraying (DGS) conditions into the water are discussed in detail. Analytical and numerical models for the prediction of momentum and complex heat exchange, that includes radiative effects of heat transfer between the FeAl particle and the D-gun barrel wall and phase transformations due to melting and evaporation of the FeAl phase, are analyzed. Phase transformations identified during the DGS process impose the limit of FeAl grain size, which is required to maintain a solid state of aggregation during a collision with the substrate material. The identification of the characteristic time values for particle acceleration in the supersonic gas detonation flux, their convective heating and heat diffusion enable to assess the aggregation state of FeAl particles sprayed into water under certain DGS conditions.
Activated Carbons Obtained from Orange Peels, Coffee Grounds, and Sunflower Husks—Comparison of Physicochemical Properties and Activity in the Alpha-Pinene Isomerization Process
Authors: Kamińska Adrianna , Miądlicki Piotr , Kiełbasa Karolina , Kujbida Marcin , Sreńscek-Nazzal Joanna , Wróbel Rafał Jan , Wróblewska Agnieszka
Published: December 2021

This work presents studies on the preparation of porous carbon materials from waste biomass in the form of orange peels, coffee grounds, and sunflower seed husks. The preparation of activated carbons from these three waste materials involved activation with KOH followed by carbonization at 800 °C in an N2 atmosphere. This way of obtaining the activated carbons is very simple and requires the application of only two reactants. Thus, this method is cheap, and it does not generate much chemical waste. The obtained activated carbons were characterized by XRD, SEM, XPS, and XRF methods. Moreover, the textural properties, acidity, and catalytic activity of these materials were descried. During catalytic tests carried out in the alpha-pinene isomerization process (the use of the activated carbons thus obtained in the process of alpha-pinene isomerization has not been described so far), the most active were activated carbons obtained from coffee grounds and orange peels. Generally, the catalytic activity of the obtained materials depended on the pore size, and the most active activated carbons had more pores with sizes of 0.7–1.0 and 1.1–1.4 nm. Moreover, the presence of potassium and chlorine ions in the pores may also be of key importance for the alpha-pinene isomerization process. On the other hand, the acidity of the surface of the tested active carbons did not affect their catalytic activity. The most favorable conditions for carrying out the alpha-pinene isomerization process were the same for the three tested activated carbons: temperature 160 °C, amount of the catalyst 5 wt.%, and reaction time 3 h. Kinetic studies were also carried out for the three tested catalysts. These studies showed that the isomerization over activated carbons from orange peels, coffee grounds, and sunflower seed husks is a first-order reaction.
Numerical and Experimental Study of Five-Layer Non-Symmetrical Paperboard Panel Stiffness
Authors: Czechowski Leszek , Kmita-Fudalej Gabriela , Szewczyk Włodzimierz , Gralewski Jacek , Bienkowska Maria
Published: December 2021

This paper concerns the analysis of five-layer corrugated paperboard subjected to a four-point bending test. The segment of paperboard was tested to determine the bending stiffness. The investigations were conducted experimentally and numerically. The non-damaging tests of bending were carried out in an elastic range of samples. The detailed layers of paperboard were modelled as an orthotropic material. The simulation of flexure was based on a finite element method using Ansys® software. Several material properties and thicknesses of papers in the samples were taken into account to analyse the influence on general stiffness. Two different discrete models based on two geometries of paperboard were considered in this study to validate the experimental stiffness. The present analysis shows the possibility of numerical modelling to achieve a good correlation with experimental results. Moreover, the results of numerical estimations indicate that modelling of the perfect structure gives a lower bending stiffness and some corrections of geometry should be implemented. The discrepancy in stiffness between both methods ranged from 3.04 to 32.88% depending on the analysed variant.
Recovering Evaluation of Narrow-Kerf Teeth of Mini Sash Gang Saws
Authors: Orlowski Kazimierz Antoni , Chuchala Daniel , Przybylinski Tomasz , Legutko Stanislaw
Published: December 2021

Sash gang saws with narrow-kerf saw blades are used in the production of glued laminate flooring elements in plants where dry technology is applied. This means that boards or friezes are sawn into top layer lamellae in dry conditions (moisture content of about 10–12%) from expensive wood species, often exotic. The object of this research was stellite-tipped teeth of narrow kerf saw blades sharpened under industrial conditions. A NIKON ECLIPSE Ti-S microscope equipped with a NIKON DS-Fi2 recording camera was used to take pictures of teeth, which were analysed in a graphical software to measure the radii of the main cutting edges. The high-quality images obtained were used to determine the values of the rounding radii of the cutting edges. It was noted that the quality of edges regenerated in industrial conditions, some of which had chipping, was lower than that of brand new saw blades.
Strengthening Mechanism and Carbide Precipitation Behavior of Nb-Mo Microalloy Medium Mn Steel
Authors: Liu Chunquan , Xiong Fen , Wang Yong , Cao Yuxin , Liu Xinbin , Xue Zhengliang , Peng Qichun , Peng Longsheng
Published: December 2021

This study investigates the strengthening mechanism and carbide precipitation behavior of medium Mn steel with Nb-Mo microalloy after cyclic quenching and austenite reverse transformation treatment. The results show that the Nb/Mo element not only precipitates (Nb,Mo)C in the grains, hindering the movement of dislocations and increases the strength, but also segregates at the austenite/ferrite grain boundary, thus delaying the transformation from austenite to ferrite. In addition, a large amount of nano-scale cementite is retained after cyclic quenching and austenite reverse transformation, which has a positive effect on the proportion of retained austenite in medium Mn steel. Moreover, the carbides with small size and low Mn content are dissolved, and the decomposed C and Mn content are beneficial to the nucleation of austenite during the intercritical annealing process at a temperature of 690 °C.
Effects of Fillet Weld Size and Sleeve Material Strength on the Residual Stress Distribution and Structural Safety While Implementing the New Sleeve Repair Process
Authors: Zhang Hongjie , Han Tao , Wang Yong , Wu Qian
Published: December 2021

The process optimization and structural safety improvement of the in-service repair welding of the X80 pipeline are very important. In this paper, the temperature, microstructure, and stress distribution were analyzed using the combination of TMM (thermal-metallurgical-mechanical) simulations and the corresponding verification experiments. The effects of the sleeve material strength and the fillet weld size were discussed. The results showed that the fillet weld zone was mainly composed of ferrite and bainite when the material of the sleeve pipe was Q345B. Furthermore, the sleeve pipe’s HAZ (heat affected zone) was dominated by lath martensite, lath bainite, and granular bainite. Moreover, granular bainite and a small amount of ferrite were found in the HAZ of the X80 pipe. It was found that, as the fillet weld size increased, the welding residual stress distribution became more uniform. The hoop stress at weld toe reduced from ~860 MPa of case A to ~680 MPa of case E, and the axial stress at weld toe reduced from ~440 MPa of case A to ~380 MPa of case E. From the viewpoint of welding residual stress, fillet weld size was suggested to be larger than 1.4T. The stress concentration and the stress distribution showed a correlation with the cracking behavior. Weld re-solidification ripples on the weld surface and weld ripples between welding passes or near the weld toe could cause stress concentration and the corresponding crack initiation. Furthermore, when the material of the sleeve pipe changed from Q345B to X80, the high-level tensile stress zone was found to be enlarged. The hoop stress at weld toe increased from ~750 to ~800 MPa, and the axial stress at weld toe increased from ~500 to ~600 MPa. After implementing the new sleeve repair welding process where X80 replaces the material of sleeve pipe, the cracking risk in sleeve pipe will improve. From the perspective of the welding residual stress, it was concluded that the fillet weld size reduction and the sleeve material strength improvement are harmful to in-service welded structures’ safety and integrity.
Photoresponse Dimensionality of Organic Field-Effect Transistor
Authors: Vincze Tomas , Micjan Michal , Nevrela Juraj , Donoval Martin , Weis Martin
Published: December 2021

Organic field-effect transistors have been envisioned for advanced photodetectors because the organic semiconductors provide unique absorption characteristics, low-cost fabrication, or compatibility with flexible substrates. However, the response time of organic phototransistors still does not reach the required application level. Here, we report the photoresponse of copper phthalocyanine phototransistor in a steady state and under pulsed illumination. The detailed analysis based on the random walk among a field of traps was used to evaluate the dimensionality of electron transport in a device.
The Influence of Root Canal Preparation with ProTaper Next, WaveOne Gold, and Twisted Files on Dentine Crack Formation
Authors: Eliasz Wojciech , Czarnecka Beata , Surdacka Anna
Published: December 2021

(1) Background: Root canal preparation constitutes an important factor for success in endodontics. However, various complications may occur during this stage. The aim of this study was to compare the incidence of cracks within radicular dentin after instrumentation with ProTaper Next (PTN), WaveOne Gold (WOG), and Twisted Files (TF), which utilize different kinematics. (2) Methods: Eighty single-rooted teeth were classified into four groups (n = 20). Three groups were instrumented using PTN (X1, X2), WOG (Primary), and TF (SM1–3). The non-instrumented group constituted the control group. Post-preparation, the roots were sectioned 3, 6, and 9 mm from the apex using a low-speed saw (Southbay Technology Inc., San Clemente, CA, USA). The specimens were viewed through a microscope at x25 magnification (Leica M320, Wetzlar, Germany), and their surface was assessed tactilely to determine the presence of the crack. (3) Results: Partial cracks within radicular dentine were observed in all study groups (PTN: n = 4/20%, WOG: n = 3/15%, TF: n = 4/20%); no cracks were observed in the control group. No significant differences were observed among experimental groups. OR values for the incidence of cracks were: OR = 11.182 for PTN, OR = 8.2 for WOG, and OR = 8.2 for TF. (4) Conclusions: Instrumentation with PTN, WOG, and TF may result in dentinal cracks formation.
Approximation of Non-Linear Rotor Dynamic Resonance Behavior of Vertically Aligned Hydro-Units Guided by Tilting-Pad Bearings
Authors: Vetter Daniel , Hagemann Thomas , Schubert Andreas , Schwarze Hubert
Published: December 2021

Dynamic analyses of vertical hydro power plant rotors require the consideration of the non-linear bearing characteristics. This study investigates the vibrational behavior of a typical vertical machine using a time integration method that considers non-linear bearing forces. Thereby, the influence of support stiffness and unbalance magnitude is examined. The results show a rising influence of unbalance on resonance speed with increasing support stiffness. Furthermore, simulations reveal that the shaft orbit in the bearing is nearly circular for typical design constellations. This property is applied to derive a novel approximation procedure enabling the examination of non-linear resonance behavior, using linear rotor dynamic theory. The procedure considers the dynamic film pressure for determining the pad position. In addition, it is time-efficient compared to a time integration method, especially at high amplitudes when damping becomes small.
Reliability Analysis of High Concrete-Face Rockfill Dams and Study of Seismic Performance of Earthquake-Resistant Measures Based on Stochastic Dynamic Analysis
Authors: Rong Zhuo , Yu Xiang , Xu Bin , Du Xueming
Published: December 2021

The randomness of earthquake excitation has a significant impact on the seismic performance of high earth-rock dams. In this paper, the seismic performance of geosynthetic-reinforced soil structures (GRSS) of high concrete face rockfill dams (CFRDs) is evaluated from the stochastic perspective. Multiple groups of seismic ground motions are generated based on spectral expression-random function non-stationary model. Taking Gushui CFRD as an example, this study calculates the failure probability of each damage level of non-reinforce slopes and reinforce slopes based on generalized probability density evolution method (GPDEM) and reliability analysis is presented though multiple evaluation indicators. The result shows that GRSS can reduce the mild damage of CFRDs during earthquake and restrain the moderate and severe damage. The influence of vertical spacing and length of GRSS on the seismic performance is obtained, which provides a reference for the seismic design and risk analysis of CFRDs.
Immune Response after SARS-CoV-2 Vaccination in Kidney Transplant Patients
Authors: Vaiciuniene Ruta , Sitkauskiene Brigita , Bumblyte Inga Arune , Dalinkeviciene Egle , Ziginskiene Edita , Bagdonas Dovydas , Augliene Ruta , Petruliene Kristina , Bagdziuniene Irmante , Skarupskiene Inga , Stankuviene Asta , Sauseriene Jolanta , Macinskas Sarunas , Valius Leonas
Published: December 2021

Background and Objectives: The prospective study was conducted to evaluate humoral and cellular immune responses after two doses of BNT162b2 (Pfizer-BioNTech) vaccine and possible relation with other factors (medication, etc.) in kidney transplant patients. Materials and Methods: Out of 167 vaccinated patients, 136 agreed to a follow-up visit three to six weeks after vaccination. Results: Only 39 patients (29%) developed antibody response against SARS-CoV-2 (≥35.2 binding antibody units (BAU)/mL) after full vaccination. Multivariate binary logistic regression analysis showed that predictive factors for good antibody response to the COVID-19 vaccine were better kidney function, higher hemoglobin level, and no use of mycophenolate mofetil for immunosuppression. For seropositive kidney transplant patients there was a significant negative correlation between anti-SARS-CoV-2 antibody titer and CD4/CD8 ratio (Spearman’s correlation coefficient −0.4, p = 0.02), percentage of CD19+ cells (r = −0.37, p = 0.02), and a positive correlation with percentage of CD8+ cells (r = 0.4, p = 0.01). There was an increase of total leucocyte count after vaccination in the total studied population, and in the group of responders. Conclusions: Only one third of kidney transplant patients develop sufficient antibody responses after full COVID-19 vaccination with Pfizer-BioNTech. Better kidney function, higher hemoglobin level, and no use of mycophenolate mofetil for immunosuppression increases the adequacy of response. The antibody titers correlated positively with relative number of CD8+ cells and negatively with CD4/CD8 ratio in responders.
Membrane Distillation: Pre-Treatment Effects on Fouling Dynamics
Authors: Santos Paula G. , Scherer Cíntia M. , Fisch Adriano G. , Rodrigues Marco Antônio S.
Published: December 2021

In the research reported in this paper, membrane distillation was employed to recover water from a concentrated saline petrochemical effluent. According to the results, the use of membrane distillation is technically feasible when pre-treatments are employed to mitigate fouling. A mathematical model was used to evaluate the fouling mechanism, showing that the deposition of particulate and precipitated material occurred in all tests; however, the fouling dynamic depends on the pre-treatment employed (filtration, or filtration associated with a pH adjustment). The deposit layer formed by particles is not cohesive, allowing its entrainment to the bulk flow. The precipitate fouling showed a minimal tendency to entrainment. Also, precipitate fouling served as a coupling agent among adjacent particles, increasing the fouling layer cohesion.
Solid-Contact Potentiometric Anion Sensing Based on Classic Silver/Silver Insoluble Salts Electrodes without Ion-Selective Membrane
Authors: Liao Chunxian , Zhong Lijie , Tang Yitian , Sun Zhonghui , Lin Kanglong , Xu Longbin , Lyu Yan , He Dequan , He Ying , Ma Yingming , Bao Yu , Gan Shiyu , Niu Li
Published: December 2021

Current solid potentiometric ion sensors mostly rely on polymeric-membrane-based, solid-contact, ion-selective electrodes (SC-ISEs). However, anion sensing has been a challenge with respect to cations due to the rareness of anion ionophores. Classic metal/metal insoluble salt electrodes (such as Ag/AgCl) without an ion-selective membrane (ISM) offer an alternative. In this work, we first compared the two types of SC-ISEs of Cl− with/without the ISM. It is found that the ISM-free Ag/AgCl electrode discloses a comparable selectivity regarding organic chloride ionophores. Additionally, the electrode exhibits better comprehensive performances (stability, reproducibility, and anti-interference ability) than the ISM-based SC-ISE. In addition to Cl−, other Ag/AgX electrodes also work toward single and multi-valent anions sensing. Finally, a flexible Cl− sensor was fabricated for on-body monitoring the concentration of sweat Cl− to illustrate a proof-of-concept application in wearable anion sensors. This work re-emphasizes the ISM-free SC-ISEs for solid anion sensing.
Improved Peripheral and Hepatic Insulin Sensitivity after Lifestyle Interventions in Type 2 Diabetes Is Associated with Specific Metabolomic and Lipidomic Signatures in Skeletal Muscle and Plasma
Authors: Chorell Elin , Otten Julia , Stomby Andreas , Ryberg Mats , Waling Maria , Hauksson Jon , Svensson Michael , Olsson Tommy
Published: December 2021

Lifestyle interventions with weight loss can improve insulin sensitivity in type 2 diabetes (T2D), but mechanisms are unclear. We explored circulating and skeletal muscle metabolite signatures of altered peripheral (pIS) and hepatic insulin sensitivity (hIS) in overweight and obese T2D individuals that were randomly assigned a 12-week Paleolithic-type diet with (diet-ex, n = 13) or without (diet, n = 13) supervised exercise. Baseline and post-intervention measures included: mass spectrometry-based metabolomics and lipidomics of skeletal muscle and plasma; pIS and hIS; ectopic lipid deposits in the liver and skeletal muscle; and skeletal muscle fat oxidation rate. Both groups lowered BMI and total % fat mass and increased their pIS. Only the diet-group improved hIS and reduced ectopic lipids in the liver and muscle. The combined improvement in pIS and hIS in the diet-group were associated with decreases in muscle and circulating branched-chain amino acid (BCAA) metabolites, specifically valine. Improved pIS with diet-ex was instead linked to increased diacylglycerol (34:2) and triacylglycerol (56:0) and decreased phosphatidylcholine (34:3) in muscle coupled with improved muscle fat oxidation rate. This suggests a tissue crosstalk involving BCAA-metabolites after diet intervention with improved pIS and hIS, reflecting reduced lipid influx. Increased skeletal muscle lipid utilization with exercise may prevent specific lipid accumulation at sites that perturb insulin signaling.
Which Role Plays 2-Hydroxybutyric Acid on Insulin Resistance?
Authors: Sousa André P. , Cunha Diogo M. , Franco Carolina , Teixeira Catarina , Gojon Frantz , Baylina Pilar , Fernandes Ruben
Published: December 2021

Type 2 Diabetes Mellitus (T2D) is defined as a chronic condition caused by beta cell loss and/or dysfunction and insulin resistance (IR). The discovering of novel biomarkers capable of identifying T2D and other metabolic disorders associated with IR in a timely and accurate way is critical. In this review, 2-hydroxybutyric acid (2HB) is presented as that upheaval biomarker with an unexplored potential ahead. Due to the activation of other metabolic pathways during IR, 2HB is synthesized as a coproduct of protein metabolism, being the progression of IR intrinsically related to the increasing of 2HB levels. Hence, the focus of this review will be on the 2HB metabolite and its involvement in glucose homeostasis. A literature review was conducted, which comprised an examination of publications from different databases that had been published over the previous ten years. A total of 19 articles fulfilled the intended set of criteria. The use of 2HB as an early indicator of IR was separated into subjects based on the number of analytes examined simultaneously. In terms of the association between 2HB and IR, it has been established that increasing 2HB levels can predict the development of IR. Thus, 2HB has demonstrated considerable promise as a clinical monitoring molecule, not only as an IR biomarker, but also for disease follow-up throughout IR treatment.
Molecular Mechanisms Underlying the Relationship between Obesity and Male Infertility
Authors: Barbagallo Federica , Condorelli Rosita A. , Mongioì Laura M. , Cannarella Rossella , Cimino Laura , Magagnini Maria Cristina , Crafa Andrea , La Vignera Sandro , Calogero Aldo E.
Published: December 2021

In recent decades, the worldwide prevalence of obesity has risen dramatically and is currently estimated to be around 20%. Obesity is linked to an increased risk of comorbidities and premature mortality. Several studies have shown that obesity negatively impacts male fertility through various mechanisms. This review aims to investigate the molecular mechanisms through which obesity impairs male reproduction, including obesity-associated hypogonadism and its effects on spermatogenesis, chronic inflammation, and oxidative stress. Obesity negatively impacts both conventional and biofunctional sperm parameters, and it also induces epigenetic changes that can be transferred to offspring. Moreover, obesity-related diseases are linked to a dysregulation of adipocyte function and micro-environmental inflammatory processes. The dysregulated adipokines significantly influence insulin signaling, and they may also have a detrimental effect on testicular function. Sirtuins can also play an important role in inflammatory and metabolic responses in obese patients. Understanding the molecular mechanisms that are involved in obesity-induced male infertility could increase our ability to identify novel targets for the prevention and treatment of obesity and its related consequences.
Osmotically Enabled Wearable Patch for Sweat Harvesting and Lactate Quantification
Authors: Saha Tamoghna , Fang Jennifer , Mukherjee Sneha , Knisely Charles T. , Dickey Michael D. , Velev Orlin D.
Published: December 2021

Lactate is an essential biomarker for determining the health of the muscles and oxidative stress levels in the human body. However, most of the currently available sweat lactate monitoring devices require external power, cannot measure lactate under low sweat rates (such as in humans at rest), and do not provide adequate information about the relationship between sweat and blood lactate levels. Here, we discuss the on-skin operation of our recently developed wearable sweat sampling patch. The patch combines osmosis (using hydrogel discs) and capillary action (using paper microfluidic channel) for long-term sweat withdrawal and management. When subjects are at rest, the hydrogel disc can withdraw fluid from the skin via osmosis and deliver it to the paper. The lactate amount in the fluid is determined using a colorimetric assay. During active sweating (e.g., exercise), the paper can harvest sweat even in the absence of the hydrogel patch. The captured fluid contains lactate, which we quantify using a colorimetric assay. The measurements show the that the total number of moles of lactate in sweat is correlated to sweat rate. Lactate concentrations in sweat and blood correlate well only during high-intensity exercise. Hence, sweat appears to be a suitable biofluid for lactate quantification. Overall, this wearable patch holds the potential of providing a comprehensive analysis of sweat lactate trends in the human body.
A Cascaded MEMS Amplitude Demodulator for Large Dynamic Range Application in RF Receiver
Authors: Yan Hao , Liao Xiaoping , Li Chenglin , Chen Chen
Published: December 2021

An amplitude demodulator with a large dynamic range, based on microelectromechanical systems (MEMS), is proposed in this paper. It is implemented as a cascade of a capacitive and a thermoelectric sensor. Two types of the transducer can improve the measurement range and enhance the overload capacity. This MEMS-based demodulation is realized by utilizing the square law relationship and the low-pass characteristic during the electromechanical and thermoelectric conversion. The fabrication of this device is compatible with the GaAs monolithic microwave integrated circuit (MMIC) process. Experiments show that this MEMS demodulator can realize the direct demodulation of an amplitude modulation (AM) signal with a carrier frequency of 0.35–10 GHz, and cover the power range from 0 to 23 dBm. This MEMS demodulator has the advantages of high power handling capability and zero DC power consumption.
Coal Moisture Variations in Response to Rainfall Events in Mine and Coal-Fired Power Plant Stockpiles—Part 2: Evaporation
Authors: Campbell Quentin Peter , le Roux Marco , Nakhaei Fardis
Published: December 2021

Additional moisture added in coal stockpiles due to rain and other climatic processes causes a significant problem worldwide, which leads to not only decrease in the heating value of the coal but also creates an extra efficiency penalty. Therefore, it is important to make some predictions for control of coal moisture within stockpiles after the rainfall. When the rain falls on the stockpile, it either runs off the surface or infiltrates the stockpile. The infiltrated water may evaporate from the surface, drain or stay within the stockpile. The aims of this study (parts 1 and 2) are to describe and compare the changes in coal moisture content following rainfall events. The mechanisms of runoff, infiltration and drainage after rainfall were described in the first paper of this series. In part 2 the influence of coal particle size and ambient conditions on the rate and depth of moisture evaporation within the stockpile is investigated. The laboratory experiments showed cyclic events of adsorbing moisture overnight and desorbing this moisture during the day as part of the coal surface evaporation process. The rate of evaporation from the surface of the fine coal stockpile was faster than the coarse stockpile; however, the coarse stockpile experienced a more efficient evaporation process because of its porous structure. Fine coal beds experienced evaporation only near the surface, while the maximum influencing layer of evaporation is a depth of 0.4 cm below the surface in coarse coal beds.
Methodologies for the Possible Integral Generation of Geopolymers Based on Copper Tailings
Authors: Castillo Hengels , Collado Humberto , Droguett Thomas , Sánchez Sebastián , Vesely Mario , Garrido Pamela , Palma Sergio
Published: December 2021

It is of interest to study the use of copper tailings as a raw material to generate geopolymers due to the exorbitant amounts of existing tailings deposits, which also produce different risks to nearby communities. Therefore, using this industrial waste as construction material would result in several environmental and economic benefits. Due to the above, it is necessary to perform laboratory tests that account for the relevant variables to obtain fresh geopolymer pastes with good consistency, and to obtain hardened geopolymers with good mechanical strength. This report gives an account of the experimental work carried out in the laboratory of the CIMS Sustainable Mining Research Center of the Engineering Consulting Company JRI, exposing the preliminary results observed in the generation of geopolymers by means of seven different methods using copper tailings and NaOH. Of the seven methods evaluated in the laboratory, it was observed that two of them deliver better results from a qualitative point of view, where the influence of the curing stage stands out, reflecting that temperature is one of the critical variables for the formation of geopolymers based on copper tailings and NaOH. The best means to work the mixtures should be studied to optimize the solubility of the NaOH and, therefore, the dissolution of the aluminosilicates in the tailings.
Secondary Metabolite Profiling, Anti-Inflammatory and Hepatoprotective Activity of Neptunia triquetra (Vahl) Benth
Authors: Wagay Nasir Aziz , Rafiq Shah , Rather Mohammad Aslam , Tantray Younas Rasheed , Lin Feng , Wani Shabir Hussain , El-Sabrout Ahmed M. , Elansary Hosam O. , Mahmoud Eman A.
Published: December 2021

The present study aimed to analyze the phytoconstituents of Neptunia triquetra (Vahl) Benth. Anti-inflammatory and hepatoprotective activities of ethanol (EE), chloroform (CE) and dichloromethane (DCME) of stem extracts were evaluated using in vivo experimental models. The extracts were analyzed for phytoconstituents using GC-HRMS. Anti-inflammatory activity of CE, EE and DCME was accessed using carrageenan-induced paw oedema, cotton pellet-induced granuloma and the carrageenan-induced air-pouch model in Wistar albino rats. The hepatotoxicity-induced animal models were investigated for the biochemical markers in serum (AST, ALT, ALP, GGT, total lipids and total protein) and liver (total protein, total lipids, GSH and wet liver weight). In the in vivo study, animals were divided into different groups (six in each group) for accessing the anti-inflammatory and hepatoprotective activity, respectively. GC-HRMS analysis revealed the presence of 102 compounds, among which 24 were active secondary metabolites. In vivo anti-inflammatory activity of stem extracts was found in the order: indomethacin > chloroform extract (CE) > dichloromethane extract (DCME) > ethanolic extract (EE), and hepatoprotective activity of stem extracts in the order: CE > silymarin > EE > DCME. The results indicate that N. triquetra stem has a higher hepatoprotective effect than silymarin, however the anti-inflammatory response was in accordance with or lower than indomethacin.
[18F]Nifene PET/CT Imaging in Mice: Improved Methods and Preliminary Studies of α4β2* Nicotinic Acetylcholinergic Receptors in Transgenic A53T Mouse Model of α-Synucleinopathy and Post-Mortem Human Parkinson’s Disease
Authors: Campoy Anthony-David T. , Liang Christopher , Ladwa Reisha M. , Patel Krystal K. , Patel Ishani H. , Mukherjee Jogeshwar
Published: December 2021

We report [18F]nifene binding to α4β2* nicotinic acetylcholinergic receptors (nAChRs) in Parkinson’s disease (PD). The study used transgenic Hualpha-Syn(A53T) PD mouse model of α-synucleinopathy for PET/CT studies in vivo and autoradiography in vitro. Additionally, postmortem human PD brain sections comprising of anterior cingulate were used in vitro to assess translation to human studies. Because the small size of mice brain poses challenges for PET imaging, improved methods for radiosynthesis of [18F]nifene and simplified PET/CT procedures in mice were developed by comparing intravenous (IV) and intraperitoneal (IP) administered [18F]nifene. An optimal PET/CT imaging time of 30–60 min post injection of [18F]nifene was established to provide thalamus to cerebellum ratio of 2.5 (with IV) and 2 (with IP). Transgenic Hualpha-Syn(A53T) mice brain slices exhibited 20–35% decrease while in vivo a 20–30% decrease of [18F]nifene was observed. Lewy bodies and α-synuclein aggregates were confirmed in human PD brain sections which lowered the [18F]nifene binding by more than 50% in anterior cingulate. Thus [18F]nifene offers a valuable tool for PET imaging studies of PD.
Non-PGM Electrocatalysts for PEM Fuel Cells: A DFT Study on the Effects of Fluorination of FeNx-Doped and N-Doped Carbon Catalysts
Authors: Cherif Mohamed , Dodelet Jean-Pol , Zhang Gaixia , Glibin Vassili P. , Sun Shuhui , Vidal François
Published: December 2021

Fluorination is considered as a means of reducing the degradation of Fe/N/C, a highly active FeNx-doped disorganized carbon catalyst for the oxygen reduction reaction (ORR) in PEM fuel cells. Our recent experiments have, however, revealed that fluorination poisons the FeNx moiety of the Fe/N/C catalytic site, considerably reducing the activity of the resulting catalyst to that of carbon only doped with nitrogen. Using the density functional theory (DFT), we clarify in this work the mechanisms by which fluorine interacts with the catalyst. We studied 10 possible FeNx site configurations as well as 2 metal-free sites in the absence or presence of fluorine molecules and atoms. When the FeNx moiety is located on a single graphene layer accessible on both sides, we found that fluorine binds strongly to Fe but that two F atoms, one on each side of the FeNx plane, are necessary to completely inhibit the catalytic activity of the FeNx sites. When considering the more realistic model of a stack of graphene layers, only one F atom is needed to poison the FeNx moiety on the top layer since ORR hardly takes place between carbon layers. We also found that metal-free catalytic N-sites are immune to poisoning by fluorination, in accordance with our experiments. Finally, we explain how most of the catalytic activity can be recovered by heating to 900 °C after fluorination. This research helps to clarify the role of metallic sites compared to non-metallic ones upon the fluorination of FeNx-doped disorganized carbon catalysts.
Isolation and Identification of Pentalenolactone Analogs from Streptomyces sp. NRRL S-4
Authors: Li Huanhuan , Li Hongji , Chen Shuo , Wu Wenhui , Sun Peng
Published: December 2021

Terpene synthases are widely distributed in Actinobacteria. Genome sequencing of Streptomyces sp. NRRL S-4 uncovered a biosynthetic gene cluster (BGC) that putatively synthesizes pentalenolactone type terpenes. Guided by genomic information, the S-4 strain was chemically investigated, resulting in the isolation of two new sesquiterpenoids, 1-deoxy-8α-hydroxypentalenic acid (1) and 1-deoxy-9β-hydroxy-11-oxopentalenic acid (2), as shunt metabolites of the pentalenolactone (3) biosynthesis pathway. Their structures and absolute configurations were elucidated by analyses of HRESIMS and NMR spectroscopic data as well as time-dependent density functional theory/electronic circular dichroism (TDDFT/ECD) calculations. Compounds 1 and 2 exhibited moderate antimicrobial activities against Gram-positive and Gram-negative bacteria. These results confirmed that the pentalenolactone pathway was functional in this organism and will facilitate efforts for exploring Actinobacteria using further genome mining strategies.
The Applications of Ultra-Thin Nanofilm for Aerospace Advanced Manufacturing Technology
Authors: Xie Guibai , Bai Hongwu , Miao Guanghui , Feng Guobao , Yang Jing , He Yun , Li Xiaojun , Li Yun
Published: December 2021

With the development of industrial civilization, advanced manufacturing technology has attracted widespread concern, including in the aerospace industry. In this paper, we report the applications of ultra-thin atomic layer deposition nanofilm in the advanced aerospace manufacturing industry, including aluminum anti-oxidation and secondary electron suppression, which are critical in high-power and miniaturization development. The compact and uniform aluminum oxide film, which is formed by thermal atomic layer deposition (ALD), can prevent the deep surface oxidation of aluminum during storage, avoiding the waste of material and energy in repetitive production. The total secondary electron yield of the C/TiN component nanofilm, deposited through plasma-enhanced atomic layer deposition, decreases 25% compared with an uncoated surface. The suppression of secondary electron emission is of great importance in solving the multipactor for high-power microwave components in space. Moreover, the controllable, ultra-thin uniform composite nanofilm can be deposited directly on the complex surface of devices without any transfer process, which is critical for many different applications. The ALD nanofilm shows potential for promoting system performance and resource consumption in the advanced aerospace manufacturing industry.
Smart Mn7+ Sensing via Quenching on Dual Fluorescence of Eu3+ Complex-Modified TiO2 Nanoparticles
Authors: Yang Wenbin , Niu Siqi , Wang Yao , Huang Linjun , Wang Shichao , Popat Ketul C. , Kipper Matt J. , Belfiore Laurence A. , Tang Jianguo
Published: December 2021

In this work, titania (TiO2) nanoparticles modified by Eu(TTA)3Phen complexes (ETP) were prepared by a simple solvothermal method developing a fluorescence Mn7+ pollutant sensing system. The characterization results indicate that the ETP cause structural deformation and redshifts of the UV-visible light absorptions of host TiO2 nanoparticles. The ETP also reduce the crystallinity and crystallite size of TiO2 nanoparticles. Compared with TiO2 nanoparticles modified with Eu3+ (TiO2-Eu3+), TiO2 nanoparticles modified with ETP (TiO2-ETP) exhibit significantly stronger photoluminescence under the excitation of 394 nm. Under UV excitation, TiO2-ETP nanoparticles showed blue and red emission corresponding to TiO2 and Eu3+. In addition, as the concentration of ETP in TiO2 nanoparticles increases, the PL intensity at 612 nm also increases. When ETP-modified TiO2 nanoparticles are added to an aqueous solution containing Mn7+, the fluorescence intensity of both TiO2 and ETP decreases. The evolution of the fluorescence intensity ratio (I1/I2) of TiO2 and ETP is linearly related to the concentration of Mn7+. The sensitivity of fluorescence intensity to Mn7+ concentration enables the design of dual fluorescence ratio solid particle sensors. The method proposed here is simple, accurate, efficient, and not affected by the environmental conditions.
Biogenic Synthesis of Iron Oxide Nanoparticles Using Enterococcus faecalis: Adsorption of Hexavalent Chromium from Aqueous Solution and In Vitro Cytotoxicity Analysis
Authors: Samuel Melvin S. , Datta Saptashwa , Chandrasekar Narendhar , Balaji Ramachandran , Selvarajan Ethiraj , Vuppala Srikanth
Published: December 2021

The biological synthesis of nanoparticles is emerging as a potential method for nanoparticle synthesis due to its non-toxicity and simplicity. In the present study, a bacterium resistant to heavy metals was isolated from a metal-contaminated site and we aimed to report the synthesis of Fe3O4 nanoparticles via co-precipitation using bacterial exopolysaccharides (EPS) derived from Enterococcus faecalis_RMSN6 strains. A three-variable Box–Behnken design was used for determining the optimal conditions of the Fe3O4 NPs synthesis process. The synthesized Fe3O4 NPs were thoroughly characterized through multiple analytical techniques such as XRD, UV-Visible spectroscopy, FTIR spectroscopy and finally SEM analysis to understand the surface morphology. Fe3O4 NPs were then probed for the Cr(VI) ion adsorption studies. The important parameters such as optimization of initial concentration of Cr(VI) ions, effects of contact time, pH of the solution and contact time on quantity of Cr(VI) adsorbed were studied in detail. The maximum adsorption capacity of the nanoparticles was found to be 98.03 mg/g. The nanoparticles could retain up to 73% of their efficiency of chromium removal for up to 5 cycles. Additionally, prepared Fe3O4 NPs in the concentration were subjected to cytotoxicity studies using an MTT assay. The investigations using Fe3O4 NPs displayed a substantial dose-dependent effect on the A594 cells. The research elucidates that the Fe3O4 NPs synthesized from EPS of E. faecalis_RMSN6 can be used for the removal of heavy metal contaminants from wastewater.