bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization and Impact of Waste Plastic Oil in a Variable Compression Ratio Diesel Engine
Authors: Wathakit Khatha , Sukjit Ekarong , Kaewbuddee Chalita , Maithomklang Somkiat , Klinkaew Niti , Liplap Pansa , Arjharn Weerachai , Srisertpol Jiraphon
Published: April 2021

The characterization of pyrolysis oil obtained from mixed waste plastics and its utilization in a compression ignition engine were investigated. The chemical compositions and physicochemical properties of distilled waste plastic oil (WPO) and crude waste plastic oil (CWPO) were analyzed. The experiment was conducted with a variable compression ratio diesel engine at various loads and compression ratios to evaluate combustion characteristics, exhaust emissions, and engine performance. The experimental results show that CWPO contains the highest percentage of carbon atoms in the C4–C11 group, while WPO contains the highest percentage of carbon atoms in the C12–C20 group, similar to the main compositions of diesel fuel. According to the preliminary study in chemical compositions and physicochemical properties, WPO and diesel fuel were selected for the engine test at different compression ratios of 16, 17, and 18 and different engine operating loads of 25%, 50%, and 75% of maximum engine torque at an engine speed of 1500 rpm. It was found that increasing the engine operating load and the compression ratio tends to increase the brake thermal efficiency. Increasing the compression ratio results in a significantly shorter delay time in a combustion state. A lower cetane index and a higher percentage of long chain carbon compounds (C12–C20) could be the main factors affecting higher NOx, CO, and HC emissions with the combustion characteristics of WPO, compared to diesel fuel. The disadvantage of emissions by the use of WPO can be alleviated when the engine is running at maximum load and a high compression ratio.
A Review of Electric Vehicle Load Open Data and Models
Authors: Amara-Ouali Yvenn , Goude Yannig , Massart Pascal , Poggi Jean-Michel , Yan Hui
Published: April 2021

The field of electric vehicle charging load modelling has been growing rapidly in the last decade. In light of the Paris Agreement, it is crucial to keep encouraging better modelling techniques for successful electric vehicle adoption. Additionally, numerous papers highlight the lack of charging station data available in order to build models that are consistent with reality. In this context, the purpose of this article is threefold. First, to provide the reader with an overview of the open datasets available and ready to be used in order to foster reproducible research in the field. Second, to review electric vehicle charging load models with their strengths and weaknesses. Third, to provide suggestions on matching the models reviewed to six datasets found in this research that have not previously been explored in the literature. The open data search covered more than 860 repositories and yielded around 60 datasets that are relevant for modelling electric vehicle charging load. These datasets include information on charging point locations, historical and real-time charging sessions, traffic counts, travel surveys and registered vehicles. The models reviewed range from statistical characterization to stochastic processes and machine learning and the context of their application is assessed.
Alternating Current Loss of Superconductors Applied to Superconducting Electrical Machines
Authors: Zhang Hongye , Wen Zezhao , Grilli Francesco , Gyftakis Konstantinos , Mueller Markus
Published: April 2021

Superconductor technology has recently attracted increasing attention in power-generation- and electrical-propulsion-related domains, as it provides a solution to the limited power density seen by the core component, electrical machines. Superconducting machines, characterized by both high power density and high efficiency, can effectively reduce the size and mass compared to conventional machine designs. This opens the way to large-scale purely electrical applications, e.g., all-electrical aircrafts. The alternating current (AC) loss of superconductors caused by time-varying transport currents or magnetic fields (or both) has impaired the efficiency and reliability of superconducting machines, bringing severe challenges to the cryogenic systems, too. Although much research has been conducted in terms of the qualitative and quantitative analysis of AC loss and its reduction methods, AC loss remains a crucial problem for the design of highly efficient superconducting machines, especially for those operating at high speeds for future aviation. Given that a critical review on the research advancement regarding the AC loss of superconductors has not been reported during the last dozen years, especially combined with electrical machines, this paper aims to clarify its research status and provide a useful reference for researchers working on superconducting machines. The adopted superconducting materials, analytical formulae, modelling methods, measurement approaches, as well as reduction techniques for AC loss of low-temperature superconductors (LTSs) and high-temperature superconductors (HTSs) in both low- and high-frequency fields have been systematically analyzed and summarized. Based on the authors’ previous research on the AC loss characteristics of HTS coated conductors (CCs), stacks, and coils at high frequencies, the challenges for the existing AC loss quantification methods have been elucidated, and multiple suggestions with respect to the AC loss reduction in superconducting machines have been put forward. This article systematically reviews the qualitative and quantitative analysis methods of AC loss as well as its reduction techniques in superconductors applied to electrical machines for the first time. It is believed to help deepen the understanding of AC loss and deliver a helpful guideline for the future development of superconducting machines and applied superconductivity.
Sensitivity-Based Optimization of Interior Permanent Magnet Synchronous Motor for Torque Characteristic Enhancement
Authors: Ahmadi Sajjad , Lubin Thierry , Vahedi Abolfazl , Taghavi Nasser
Published: April 2021

This paper presents a multi-objective optimal rotor design for an interior permanent magnet synchronous motor (IPMSM) based on finite element analysis. Due to the importance of torque characteristic in electromagnetic design of IPMSMs, the main efforts of this study are focused on finding a proper trade-off for its torque profile challenges. In this regard, in order to attain high average torque and low torque ripple, the influence of several key factors, such as the permanent magnet (PM) arrangements, PM positions and PM sizes, are examined. Subsequently, according to the outcomes of the performed sensitivity analysis, the appropriate variation interval of the parameters as well as their initial values is determined. Employing such a deterministic optimization algorithm, which does not need large sample points, minimizes the finite element computational cost and leads to accelerate the convergence process. The two-dimensional finite element model (FEM) of an IPMSM is used to perform a sensitivity analysis and establish a multi-objective FEM-based optimization.
Study of the Effect of an Environmentally Friendly Flood Risk Reduction Approach on the Oman Coastlines during the Gonu Tropical Cyclone (Case Study: The Coastline of Sur)
Authors: Banan-Dallalian Masoud , Shokatian-Beiragh Mehrdad , Golshani Aliasghar , Mojtahedi Alireza , Lotfollahi-Yaghin Mohammad Ali , Akib Shatirah
Published: April 2021

Tropical cyclones may be destructive in the coastal region, such as the Gonu tropical cyclone, which affected the Arabian Peninsula and parts of southern Iran in 2007. In this study, a coupled MIKE 21/3 HD/SW (hydrodynamic/spectral wave) model was used to simulate the inland flooding inside the Sur port during the Gonu tropical cyclone. The MIKE 21 Cyclone Wind Generation (CWG) tool was utilized to generate the cyclone’s wind and pressure field. The required input data were obtained from the International Best Track Archive for Climate Stewardship (IBTrACS) and imported into the CWG tool. In this study, the wind and pressure fields were compared between the analytical vortex model and European Centre for Medium-Range Weather Forecasts (ECMWF) data during the Gonu cyclone passage. Moreover, by developing a new model, artificial Mangroves’ effect on inland flooding was investigated. The results show that, contrary to the ECMWF data, the analytical vortex models well captured the storm event’s wind and pressure field. Furthermore, the flood hazard is calculated based on the inundation depth, flow velocity, and area’s vulnerability. The flood hazard map shows that 5% of the coast is at high-risk, 49% is at medium-risk, and 46% is at low-risk class in the Sur port. By applying Mangroves as flood risk reduction, the high-risk area is almost completely removed. However, medium and low-risk zones increase by 50% and 50%, respectively. This information could be helpful in disaster risk reduction and coastal management in the future.
Optimization of Synthetic Media Composition for Kluyveromyces marxianus Fed-Batch Cultivation
Authors: Dubencovs Konstantins , Liepins Janis , Suleiko Arturs , Suleiko Anastasija , Vangravs Reinis , Kassaliete Jana , Scerbaka Rita , Grigs Oskars
Published: April 2021

The Kluyveromyces marxianus yeast recently has gained considerable attention due to its applicability in high-value-added product manufacturing. In order to intensify the biosynthesis rate of a target product, reaching high biomass concentrations in the reaction medium is mandatory. Fed-batch processes are an attractive and efficient way how to achieve high cell densities. However, depending on the physiology of the particular microbial strain, an optimal media composition should be used to avoid by-product synthesis and, subsequently, a decrease in overall process effi-ciency. Thus, the aim of the present study was to optimise the synthetic growth medium and feeding solution compositions (in terms of carbon, nitrogen, phosphorous, magnesium, and calcium concentrations) for high cell density K. marxianus fed‑batch cultivations. Additionally, the biomass yields from the vitamin mixture and other macro/microelements were identified. A model predictive control algorithm was successfully applied for a fed-batch cultivation control. Biomass growth and substrate consumption kinetics were compared with the mathematical model predictions. Finally, 2‑phenylethanol biosynthesis was induced and its productivity was estimated. The determined optimal macronutrient ratio for K. marxianus biomass growth was identified as C:N:P = 1:0.07:0.011. The maximal attained yeast biomass concentration was close to 70 g·L-1 and the 2-PE biosynthesis rate was 0.372 g·L−1·h−1, with a yield of 74% from 2-phenylalanine.
Numerical Study of Heat Transfer and Speed Air Flow on Performance of an Auto-Ventilated Disc Brake
Authors: García-León R. A. , Afanador-García N. , Gómez-Camperos J. A.
Published: April 2021

In the braking system, the heat dissipation generated by the friction between the disc and pad should be evacuated as quickly as possible. In this work, five common different automotive disc brakes were studied through mathematical theories of heat transfer and numerical methods using the ANSYS software. In addition, a direct comparison between experimental, theoretical, and simulation values found in the open literature was performed to propose a disc brake with an improved geometry in terms of dissipation of heat transfer. The numerical results were considered to propose two possible solutions of disc brake geometries using N-38 ventilation blades used in aeronautic engineering. An improvement in temperature dissipation was achieved by approximately 23.8% compared to the five geometries analyzed with a simple type N-38 ventilation blade. The heat dissipation in the brakes strongly depends on the geometry of the disc, the geometry of the blades, the material from which it is manufactured, the material of the pad, the weight of the vehicle, and the operating conditions, as can be verified with mathematical calculations and experiments. The results obtained demonstrate that the discs can be used effectively in extreme working conditions (80 km/h and 33°C), without affecting the safety of the occupants and the braking system.
Effect of Drying Methods on Volatile Compounds of Burdock (Arctium lappa L.) Root Tea as Revealed by Gas Chromatography Mass Spectrometry-Based Metabolomics
Authors: Xia Junjie , Guo Zili , Fang Sheng , Gu Jinping , Liang Xianrui
Published: April 2021

Burdock (Arctium lappa L.) is one of the nutritional foods widely planted in many countries. Dried burdock root (BR) is available as a herbal tincture and tea in many Asian countries with good flavor and taste. In this study, the volatile components in dried BR were identified and the effects of different drying methods on the volatile components were investigated by HS-GC-MS method. A total of 49 compounds were identified. Different drying methods including hot-air drying (HD, at 50, 60, 70, and 80 °C), vacuum drying (VD, at 50, 60, 70, and 80 °C), sunlight drying (SD), natural drying (ND), and vacuum freeze drying (VFD) were evaluated by HS-GC-MS-based metabolomics method. Results showed that different drying methods produced different effects on the volatile compounds. It was observed that 2,3-pentanedione, 1-(1H-pyrrol-2-yl)-ethanone, furfural, and heptanal were detected at higher concentrations in HD 80 and VD 70. The traditional HD and SD methods produced more flavor substances than VFD. The BR treated by the VFD method could maintain the shape of the fresh BR pieces while HD50 and VD80 methods could maintain the color of fresh BR pieces. These findings could help better understand the flavor of the corresponding processed BR and provide a guide for the drying and processing of BR tea.
Finite Element Method for Freezing and Thawing Industrial Food Processes
Authors: Fadiji Tobi , Ashtiani Seyed-Hassan Miraei , Onwude Daniel I. , Li Zhiguo , Opara Umezuruike Linus
Published: April 2021

Freezing is a well-established preservation method used to maintain the freshness of perishable food products during storage, transportation and retail distribution; however, food freezing is a complex process involving simultaneous heat and mass transfer and a progression of physical and chemical changes. This could affect the quality of the frozen product and increase the percentage of drip loss (loss in flavor and sensory properties) during thawing. Numerical modeling can be used to monitor and control quality changes during the freezing and thawing processes. This technique provides accurate predictions and visual information that could greatly improve quality control and be used to develop advanced cold storage and transport technologies. Finite element modeling (FEM) has become a widely applied numerical tool in industrial food applications, particularly in freezing and thawing processes. We review the recent studies on applying FEM in the food industry, emphasizing the freezing and thawing processes. Challenges and problems in these two main parts of the food industry are also discussed. To control ice crystallization and avoid cellular structure damage during freezing, including physicochemical and microbiological changes occurring during thawing, both traditional and novel technologies applied to freezing and thawing need to be optimized. Mere experimental designs cannot elucidate the optimum freezing, frozen storage, and thawing conditions. Moreover, these experimental procedures can be expensive and time-consuming. This review demonstrates that the FEM technique helps solve mass and heat transfer equations for any geometry and boundary conditions. This study offers promising insight into the use of FEM for the accurate prediction of key information pertaining to food processes.
Effect of Sodium Selenite, Selenium Yeast, and Bacterial Enriched Protein on Chicken Egg Yolk Color, Antioxidant Profiles, and Oxidative Stability
Authors: Muhammad Aliyu Ibrahim , Mohamed Dalia Abd Alla , Chwen Loh Teck , Akit Henny , Samsudin Anjas Asmara
Published: April 2021

The chicken egg is one of nature’s flawlessly preserved biological products, recognized as an excellent source of nutrients for humans. Selenium (Se) is an essential micro-element that plays a key role in biological processes. Organic selenium can be produced biologically by the microbial reduction of inorganic Se (sodium selenite). Therefore, the possibility of integrating Se enriched bacteria as a supplement in poultry feed can provide an interesting source of organic Se, thereby offering health-related advantages to humans. In this study, bacterial selenoproteins from Stenotrophomonas maltophilia was used as a dietary supplement with other Se sources in Lohman brown Classic laying hens to study the egg yolk color, egg yolk and breast antioxidant profile, oxidative stability, and storage effect for fresh and stored egg yolk at 4 ± 2 °C for 14-days. The results showed that dietary Se supplementation significantly (p < 0.05) improved egg yolk color, the antioxidant profile of egg yolk, and breast meat (total carotenoid and phenol content). When the Se treated groups were compared to control groups, there was a significant (p < 0.05) decrease in total cholesterol in fresh and stored egg yolk and breast muscle. In hens that were fed ADS18-Se, the primary oxidation products (MDA) concentrations in the eggs, breast, and thigh muscle, and plasma were significantly (p < 0.05) lower. However, the MDA content increased (p < 0.05) with an extended storage time in egg yolk. In comparison to inorganic Se and basal diets, egg yolk from hens fed organic Se remained fresh for two weeks. The egg yolk color, antioxidant profile, and oxidative status of egg yolk and tissue improve with dietary Se organic supplementation (ADS18 > Se-Yeast). The source of supplemented organic Se is critical for egg enrichment and antioxidant properties. As a result, ‘‘functional eggs’’ enriched with organic Se becomes possible to produce.
An Analysis of Food Waste in Czech Households—A Contribution to the International Reporting Effort
Authors: Nováková Petra , Hák Tomáš , Janoušková Svatava
Published: April 2021

Food waste originating in households in the Czech Republic is an important but unknown issue. Due to the country’s membership among the most developed economies (European Union, OECD) and its commitments towards UN Sustainable Development Goals, the government must, inter alia, significantly reduce household food waste. However, reliable data and indicators based on internationally agreed approaches and methods have been missing so far. This article brings original results from a survey comprising over 400 Czech households based on the kitchen diaries method showing that, on average, surveyed households discarded 2.6 kg (1.1 kg per capita) weekly. After extrapolation, the total food waste was estimated to be 135.7 kg per household or 57.1 kg per capita annually. Half of the total food waste from surveyed households was thrown into municipal bins for mixed waste. Despite a relatively low total food waste stream, its disposal needs substantial improvement to meet national and international regulations as well as sustainability criteria.
Post-Harvest Treatment with Methyl Jasmonate Impacts Lipid Metabolism in Tomato Pericarp (Solanum lycopersicum L. cv. Grape) at Different Ripening Stages
Authors: Rivero Meza Silvia Leticia , de Castro Tobaruela Eric , Benedetti Pascoal Grazieli , Louro Massaretto Isabel , Purgatto Eduardo
Published: April 2021

The application of exogenous jasmonate can stimulate the production of ethylene, carotenoids, and aroma compounds and accelerate fruit ripening. These alterations improve fruit quality and make fruit desirable for human consumption. However, fruit over-ripening results in large losses of fruit crops. This problem is overcome by applying 1-methylcyclopropene to the fruits, due to its capacity to block the ethylene receptors, suppressing fruit ripening. In this study, treatments with only 1-methylcyclopropene and both 1-methylcyclopropene and methyl jasmonate were administered to observe whether exogenous methyl jasmonate can improve the metabolite levels in fruits with blocked ethylene receptors. Fruit pericarps were analyzed at 4, 10, and 21 days after harvest (DAH) and compared with untreated fruits. The post-harvest treatments affected primary metabolites (sugars, organic acids, amino acids, and fatty acids) and secondary metabolites (carotenoids, tocopherols, and phytosterols). However, the lipid metabolism of the tomatoes was most impacted by the exogenous jasmonate. Fatty acids, carotenoids, tocopherols, and phytosterols showed a delay in their production at 4 and 10 DAH. Conversely, at 21 DAH, these non-polar metabolites exhibited an important improvement in their accumulation.
Induced Changes in Aroma Compounds of Foods Treated with High Hydrostatic Pressure: A Review
Authors: Lomelí-Martín Adrián , Martínez Luz María , Welti-Chanes Jorge , Escobedo-Avellaneda Zamantha
Published: April 2021

Since conventional thermal processing can have detrimental consequences on aroma compounds, non-thermal technologies such as high hydrostatic pressure (HHP) have been explored. HHP may alter the weak chemical bonds of enzymes. These changes can modify the secondary, tertiary, and quaternary structures of key enzymes in the production of aroma compounds. This can result in either an increase or decrease in their content, along with reactions or physical processes associated with a reduction of molecular volume. This article provides a comprehensive review of HHP treatment’s effects on the content of lipid-derived aroma compounds, aldehydes, alcohols, ketones, esters, lactones, terpenes, and phenols, on various food matrices of vegetable and animal origin. The content of aldehydes and ketones in food samples increased when subjected to HHP, while the content of alcohols and phenols decreased, probably due to oxidative processes. Both ester and lactone concentrations appeared to decline due to hydrolysis reactions. There is no clear tendency regarding terpenes concentration when subjected to HHP treatments. Because of the various effects of HHP on aroma compounds, an area of opportunity arises to carry out future studies that allow optimizing and controlling the effect.
A Fractional SAIDR Model in the Frame of Atangana–Baleanu Derivative
Authors: Uçar Esmehan , Uçar Sümeyra , Evirgen Fırat , Özdemir Necati
Published: April 2021

It is possible to produce mobile phone worms, which are computer viruses with the ability to command the running of cell phones by taking advantage of their flaws, to be transmitted from one device to the other with increasing numbers. In our day, one of the services to gain currency for circulating these malignant worms is SMS. The distinctions of computers from mobile devices render the existing propagation models of computer worms unable to start operating instantaneously in the mobile network, and this is particularly valid for the SMS framework. The susceptible–affected–infectious–suspended–recovered model with a classical derivative (abbreviated as SAIDR) was coined by Xiao et al., (2017) in order to correctly estimate the spread of worms by means of SMS. This study is the first to implement an Atangana–Baleanu (AB) derivative in association with the fractional SAIDR model, depending upon the SAIDR model. The existence and uniqueness of the drinking model solutions together with the stability analysis are shown through the Banach fixed point theorem. The special solution of the model is investigated using the Laplace transformation and then we present a set of numeric graphics by varying the fractional-order θ with the intention of showing the effectiveness of the fractional derivative.
Factors Associated with Frailty According to Gender of Older Adults Living Alone
Authors: Jang Hye-Young , Kim Ji-Hye
Published: April 2021

This study was conducted to identify the factors associated with frailty according to gender of older adults living alone in Korea. Data from the National Survey of the Living Conditions of Korean Elderly in 2017 were used. Participants were 2340 older adults who live alone. Frailty was determined based on the frailty criteria developed by van Kan et al. that consist of fatigue, resistance, ambulation, and illness. The collected data were analyzed using descriptive statistics, chi-squared test, t-test, Jonckheere–Terpstra test and multinomial logistic regression. Among the older men living alone, 47.7% were in the pre-frail and 5.1% were in the frail. On the other hand, 51.8% were in the pre-frail and 12.2% were in the frail among the older women living alone. The factors associated with frailty according to gender are as follows. In males, depressive symptoms, limitation in IADL, and number of medications in pre-frail; BMI, limitation in IADL, and number of chronic diseases in frail. In females, depressive symptoms, number of chronic diseases, age, and nutritional status in pre-frail; limitation in IADL, depressive symptoms, age, number of chronic diseases, number of medications, nutritional status in frail. Based on the findings of this study, it is considered necessary to approach frailty management considering gender as well as the classification of frailty.
Harnessing Stakeholder Perspectives and Experience to Address Nutrition Risk in Community-Dwelling Older Adults
Authors: Chan Catherine B. , Popeski Naomi , Gramlich Leah , Atkins Marlis , Basualdo-Hammond Carlota , Stadnyk Janet , Keller Heather
Published: April 2021

Community-dwelling, older adults have a high prevalence of nutrition risk but strategies to mitigate this risk are not routinely implemented. Our objective was to identify opportunities for the healthcare system and community organizations to combat nutrition risk in this population in the jurisdiction of Alberta, Canada. An intersectoral stakeholder group that included patient representatives was convened to share perspectives and experiences and to identify problems in need of solutions using a design thinking approach. Results: Two main themes emerged from the workshop: (1) lack of awareness and poor communication of the importance of nutrition risk between healthcare providers and from healthcare providers to patients and (2) the necessity to work in partnerships comprised of patients, community organizations, healthcare providers and the health system. Conclusion: Improving awareness, prevention and treatment of malnutrition in community-dwelling older adults requires intersectoral cooperation between patients, healthcare providers and community-based organizations.
Facility Attractiveness and Social Vulnerability Impacts on Spatial Accessibility to Opioid Treatment Programs in South Carolina
Authors: Bozorgi Parisa , Eberth Jan M. , Eidson Jeannie P. , Porter Dwayne E.
Published: April 2021

Opioid dependence and opioid-related mortality have been increasing in recent years in the United States. Available and accessible treatments may result in a reduction of opioid-related mortality. This work describes the geographic variation of spatial accessibility to opioid treatment programs (OTPs) and identifies areas with poor access to care in South Carolina. The study develops a new index of access that builds on the two-step floating catchment area (2SFCA) method, and has three dimensions: a facility attractiveness index, defined by services rendered incorporated into the Huff Model; a facility catchment area, defined as a function of facility attractiveness to account for variable catchment size; and a Social Vulnerability Index (SVI) to account for nonspatial factors that mitigate or compound the impacts of spatial access to care. Results of the study indicate a significant variation in access to OTPs statewide. Spatial access to OTPs is low across the entire state except for in a limited number of metropolitan areas. The majority of the population with low access (85%) live in areas with a moderate-to-high levels of social vulnerability. This research provides more realistic estimates of access to care and aims to assist policymakers in better targeting disadvantaged areas for OTP program expansion and resource allocation.
Arpin Regulates Migration Persistence by Interacting with Both Tankyrases and the Arp2/3 Complex
Authors: Simanov Gleb , Dang Irene , Fokin Artem I. , Oguievetskaia Ksenia , Campanacci Valérie , Cherfils Jacqueline , Gautreau Alexis M.
Published: April 2021

During cell migration, protrusion of the leading edge is driven by the polymerization of Arp2/3-dependent branched actin networks. Migration persistence is negatively regulated by the Arp2/3 inhibitory protein Arpin. To better understand Arpin regulation in the cell, we looked for its interacting partners and identified both Tankyrase 1 and 2 (TNKS) using a yeast two-hybrid screening and coimmunoprecipitation with full-length Arpin as bait. Arpin interacts with ankyrin repeats of TNKS through a C-terminal-binding site on its acidic tail, which overlaps with the Arp2/3-binding site. Arpin was found to dissolve the liquid–liquid phase separation of TNKS upon overexpression. To uncouple the interactions of Arpin with TNKS and Arp2/3, we introduced point mutations in the Arpin tail and attempted to rescue the increased migration persistence of the Arpin knockout cells using random plasmid integration or compensating knock-ins at the ARPIN locus. Arpin mutations impairing interactions with either Arp2/3 or TNKS were insufficient to fully abolish Arpin activity. Only the mutation that affected both interactions rendered Arpin completely inactive, suggesting the existence of two independent pathways, whereby Arpin controls the migration persistence.
Genome-Wide Identification and Characterization of TALE Superfamily Genes in Soybean (Glycine max L.)
Authors: Wang Liang , Yang Xinyu , Gao Yingqi , Yang Shouping
Published: April 2021

The three-amino-acid-loop-extension (TALE) superfamily genes broadly existed in plants, which played important roles in plant growth, development and abiotic stress responses. In this study, we identified 68 Glycine max TALE (GmTALE) superfamily members. Phylogenetic analysis divided the GmTALE superfamily into the BEL1-like (BLH/BELL homeodomain) and the KNOX (KNOTTED-like homeodomain) subfamilies. Moreover, the KNOX subfamily could be further categorized into three clades (KNOX Class I, KNOX Class II and KNOX Class III). The GmTALE genes showed similarities in the gene structures in the same subfamily or clade, whose coding proteins exhibited analogous motif and conserved domain compositions. Besides, synteny analyses and evolutionary constraint evaluations of the TALE members among soybean and different species provided more clues for GmTALE superfamily evolution. The cis-element analyses in gene promoter regions and relevant gene expression profiling revealed different regulating roles of GmTALE genes during soybean plant development, saline and dehydration stresses. Genome-wide characterization, evolution, and expression profile analyses of GmTALE genes can pave the way for future gene functional research and facilitate their roles for applications in genetic improvement on soybean in saline and dehydration stresses.
Cerebral Apolipoprotein D Exits the Brain and Accumulates in Peripheral Tissues
Authors: Desmarais Frederik , Hervé Vincent , Bergeron Karl F. , Ravaut Gaétan , Perrotte Morgane , Fyfe-Desmarais Guillaume , Rassart Eric , Ramassamy Charles , Mounier Catherine
Published: April 2021

Apolipoprotein D (ApoD) is a secreted lipocalin associated with neuroprotection and lipid metabolism. In rodent, the bulk of its expression occurs in the central nervous system. Despite this, ApoD has profound effects in peripheral tissues, indicating that neural ApoD may reach peripheral organs. We endeavor to determine if cerebral ApoD can reach the circulation and accumulate in peripheral tissues. Three hours was necessary for over 40% of all the radiolabeled human ApoD (hApoD), injected bilaterally, to exit the central nervous system (CNS). Once in circulation, hApoD accumulates mostly in the kidneys/urine, liver, and muscles. Accumulation specificity of hApoD in these tissues was strongly correlated with the expression of lowly glycosylated basigin (BSG, CD147). hApoD was observed to pass through bEnd.3 blood brain barrier endothelial cells monolayers. However, cyclophilin A did not impact hApoD internalization rates in bEnd.3, indicating that ApoD exit from the brain is either independent of BSG or relies on additional cell types. Overall, our data showed that ApoD can quickly and efficiently exit the CNS and reach the liver and kidneys/urine, organs linked to the recycling and excretion of lipids and toxins. This indicated that cerebral overexpression during neurodegenerative episodes may serve to evacuate neurotoxic ApoD ligands from the CNS.
Update on the Diagnostic Pitfalls of Autopsy and Post-Mortem Genetic Testing in Cardiomyopathies
Authors: Grassi Simone , Campuzano Oscar , Coll Mònica , Cazzato Francesca , Sarquella-Brugada Georgia , Rossi Riccardo , Arena Vincenzo , Brugada Josep , Brugada Ramon , Oliva Antonio
Published: April 2021

Inherited cardiomyopathies are frequent causes of sudden cardiac death (SCD), especially in young patients. Despite at the autopsy they usually have distinctive microscopic and/or macroscopic diagnostic features, their phenotypes may be mild or ambiguous, possibly leading to misdiagnoses or missed diagnoses. In this review, the main differential diagnoses of hypertrophic cardiomyopathy (e.g., athlete’s heart, idiopathic left ventricular hypertrophy), arrhythmogenic cardiomyopathy (e.g., adipositas cordis, myocarditis) and dilated cardiomyopathy (e.g., acquired forms of dilated cardiomyopathy, left ventricular noncompaction) are discussed. Moreover, the diagnostic issues in SCD victims affected by phenotype-negative hypertrophic cardiomyopathy and the relationship between myocardial bridging and hypertrophic cardiomyopathy are analyzed. Finally, the applications/limits of virtopsy and post-mortem genetic testing in this field are discussed, with particular attention to the issues related to the assessment of the significance of the genetic variants.
The Multifaceted Roles of Ku70/80
Authors: Zahid Sayma , Seif El Dahan Murielle , Iehl Florence , Fernandez-Varela Paloma , Le Du Marie-Helene , Ropars Virginie , Charbonnier Jean Baptiste
Published: April 2021

DNA double-strand breaks (DSBs) are accidental lesions generated by various endogenous or exogenous stresses. DSBs are also genetically programmed events during the V(D)J recombination process, meiosis, or other genome rearrangements, and they are intentionally generated to kill cancer during chemo- and radiotherapy. Most DSBs are processed in mammalian cells by the classical nonhomologous end-joining (c-NHEJ) pathway. Understanding the molecular basis of c-NHEJ has major outcomes in several fields, including radiobiology, cancer therapy, immune disease, and genome editing. The heterodimer Ku70/80 (Ku) is a central actor of the c-NHEJ as it rapidly recognizes broken DNA ends in the cell and protects them from nuclease activity. It subsequently recruits many c-NHEJ effectors, including nucleases, polymerases, and the DNA ligase 4 complex. Beyond its DNA repair function, Ku is also involved in several other DNA metabolism processes. Here, we review the structural and functional data on the DNA and RNA recognition properties of Ku implicated in DNA repair and in telomeres maintenance.
Antarctic Rahnella inusitata: A Producer of Cold-Stable β-Galactosidase Enzymes
Authors: Núñez-Montero Kattia , Salazar Rodrigo , Santos Andrés , Gómez-Espinoza Olman , Farah Scandar , Troncoso Claudia , Hoffmann Catalina , Melivilu Damaris , Scott Felipe , Barrientos Díaz Leticia
Published: April 2021

There has been a recent increase in the exploration of cold-active β-galactosidases, as it offers new alternatives for the dairy industry, mainly in response to the current needs of lactose-intolerant consumers. Since extremophilic microbial compounds might have unique physical and chemical properties, this research aimed to study the capacity of Antarctic bacterial strains to produce cold-active β-galactosidases. A screening revealed 81 out of 304 strains with β-galactosidase activity. The strain Se8.10.12 showed the highest enzymatic activity. Morphological, biochemical, and molecular characterization based on whole-genome sequencing confirmed it as the first Rahnella inusitata isolate from the Antarctic, which retained 41–62% of its β-galactosidase activity in the cold (4 °C–15 °C). Three β-galactosidases genes were found in the R. inusitata genome, which belong to the glycoside hydrolase families GH2 (LacZ and EbgA) and GH42 (BglY). Based on molecular docking, some of these enzymes exhibited higher lactose predicted affinity than the commercial control enzyme from Aspergillus oryzae. Hence, this work reports a new Rahnella inusitata strain from the Antarctic continent as a prominent cold-active β-galactosidase producer.
A Validated Train-Track-Bridge Model with Nonlinear Support Conditions at Bridge Approaches
Authors: Hou Wenting , Tutumluer Erol , Li Wenjing
Published: April 2021

A bridge approach, an essential component connecting a relatively rigid bridge and a more flexible track on subgrade soil, is one of the most common types of track transition zones. The tracks on a bridge deck often undergo significantly lower deformations under loading compared to the approach tracks. Even though there have been numerous efforts to understand and remediate performance deficiencies emerging from the differences in stiffness between the bridge deck and the approach, issues such as differential settlement and unsupported hanging crossties often exist. It is often difficult and expensive to try different combinations of mitigation methods in the field. Therefore, computational modeling becomes of vital importance to study dynamic responses of railroad bridge approaches. In this study, field instrumentation data were collected from the track substructure of US Amtrak’s Northeast Corridor railroad track bridge approaches. After analyses and model implementation of such comprehensive field data, an advanced train-track-bridge model is introduced and validated in this paper. Nonlinear relative displacements under varying contact forces observed between crosstie and ballast are adequately considered in the dynamic track model. The validated model is then used to simulate an Amtrak passenger train entering an open deck bridge to generate typical track transient responses and better understand dynamic behavior trends in bridge approaches. The simulated results show that near bridge location experiences much larger transient deformations, impact forces, vibration velocities and vibration accelerations. The validated track model is an analysis tool to evaluate transient responses at bridge approaches with nonlinear support; it is intended to eventually aid in developing improved track design and maintenance practices.
Marker Assisted Selection of Malic-Consuming Saccharomyces cerevisiae Strains for Winemaking. Efficiency and Limits of a QTL’s Driven Breeding Program
Authors: Vion Charlotte , Peltier Emilien , Bernard Margaux , Muro Maitena , Marullo Philippe
Published: April 2021

Natural Saccharomyces cerevisiae yeast strains exhibit very large genotypic and phenotypic diversity. Breeding programs that take advantage of this characteristic are widely used for selecting starters for wine industry, especially in the recent years when winemakers need to adapt their production to climate change. The aim of this work was to evaluate a marker assisted selection (MAS) program to improve malic acid consumption capacity of Saccharomyces cerevisiae in grape juice. Optimal individuals of two unrelated F1-hybrids were crossed to get a new genetic background carrying many “malic consumer” loci. Then, eleven quantitative trait loci (QTLs) already identified were used for implementing the MAS breeding program. By this method, extreme individuals able to consume more than 70% of malic acid in grape juice were selected. These individuals were tested in different enological matrixes and compared to their original parental strains. They greatly reduced the malic acid content at the end of alcoholic fermentation, they appeared to be robust to the environment, and they accelerated the ongoing of malolactic fermentations by Oenococcus oeni. This study illustrates how MAS can be efficiently used for selecting industrial Saccharomyces cerevisiae strains with outlier properties for winemaking.
Favorable Effects of Voriconazole Trough Concentrations Exceeding 1 μg/mL on Treatment Success and All-Cause Mortality: A Systematic Review and Meta-Analysis
Authors: Hanai Yuki , Hamada Yukihiro , Kimura Toshimi , Matsumoto Kazuaki , Takahashi Yoshiko , Fujii Satoshi , Nishizawa Kenji , Miyazaki Yoshitsugu , Takesue Yoshio
Published: April 2021

This systematic review and meta-analysis examined the optimal trough concentration of voriconazole for adult patients with invasive fungal infections. We used stepwise cutoffs of 0.5–2.0 μg/mL for efficacy and 3.0–6.0 μg/mL for safety. Studies were included if they reported the rates of all-cause mortality and/or treatment success, hepatotoxicity, and nephrotoxicity according to the trough concentration. Twenty-five studies involving 2554 patients were included. The probability of mortality was significantly decreased using a cutoff of ≥1.0 μg/mL (odds ratio (OR) = 0.34, 95% confidence interval (CI) = 0.15–0.80). Cutoffs of 0.5 (OR = 3.48, 95% CI = 1.45–8.34) and 1.0 μg/mL (OR = 3.35, 95% CI = 1.52–7.38) also increased the treatment success rate. Concerning safety, significantly higher risks of hepatotoxicity and neurotoxicity were demonstrated at higher concentrations for all cutoffs, and the highest ORs were recorded at 4.0 μg/mL (OR = 7.39, 95% CI = 3.81–14.36; OR = 5.76, 95% CI 3.14–10.57, respectively). Although further high-quality trials are needed, our findings suggest that the proper trough concentration for increasing clinical success while minimizing toxicity is 1.0–4.0 μg/mL for adult patients receiving voriconazole therapy.
Modeling Approaches to Assess Soil Erosion by Water at the Field Scale with Special Emphasis on Heterogeneity of Soils and Crops
Authors: Raza Ahsan , Ahrends Hella , Habib-Ur-Rahman Muhammad , Gaiser Thomas
Published: April 2021

Information on soil erosion and related sedimentation processes are very important for natural resource management and sustainable farming. Plenty of models are available for studying soil erosion but only a few are suitable for dynamic soil erosion assessments at the field-scale. To date, there are no field-scale dynamic models available considering complex agricultural systems for the simulation of soil erosion. We conducted a review of 51 different models evaluated based on their representation of the processes of soil erosion by water. Secondly, we consider their suitability for assessing soil erosion for more complex field designs, such as patch cropping, strip cropping and agroforestry (alley-cropping systems) and other land management practices. Several models allow daily soil erosion assessments at the sub-field scale, such as EPIC, PERFECT, GUEST, EPM, TCRP, SLEMSA, APSIM, RillGrow, WaNuLCAS, SCUAF, and CREAMS. However, further model development is needed with respect to the interaction of components, i.e., rainfall intensity, overland flow, crop cover, and their scaling limitations. A particular shortcoming of most of the existing field scale models is their one-dimensional nature. We further suggest that platforms with modular structure, such as SIMPLACE and APSIM, offer the possibility to integrate soil erosion as a separate module/component and link to GIS capabilities, and are more flexible to simulate fluxes of matter in the 2D/3D dimensions. Since models operating at daily scales often do not consider a horizontal transfer of matter, such modeling platforms can link erosion components with other environmental components to provide robust estimations of the three-dimensional fluxes and sedimentation processes occurring during soil erosion events.
Rural Tourism Network Evaluation Based on Resource Control Ability Analysis: A Case Study of Ning’an, China
Authors: Jiang Xue , Song Xiaoya , Zhao Hongyu , Zhang Haoran
Published: April 2021

Organization of rural tourism resources is important for optimizing rural land use based on rational resource classification. Quantitative analysis was performed to evaluate the resource control ability of rural tourism networks. This was achieved by determining the resource control relationship and assessing the structure of the rural tourism network. The ability of resource control was analyzed via resource abstraction, which included the extraction of resource nodes and corridors, control scope analysis, and network structure level evaluation. The proposed approach was applied to the Ning’an in Heilongjiang Province, China, and proved to be effective for exploring the network degree and development trends in rural tourism resources. By examining the resource control ability, the spatial characteristics and development trend in rural tourism networks were quantitatively analyzed, especially the connection mode of key tourism resources, network structure analysis, and resource linking ability. The core resources showed a lack of outward ability in the network, and the secondary resource expansion ability was limited. Via resource control ability analysis, this study focused on areas with rich tourism but an unbalanced spatial structure, combining the directional characteristics of the network to provide suggestions for the optimization rural tourism resources network in other regions of the world.
Synchronization of Fractional-Order Chaotic Systems with Model Uncertainty and External Disturbance
Authors: Guo Rongwei , Zhang Yaru , Jiang Cuimei
Published: April 2021

This paper is concerned with complete synchronization of fractional-order chaotic systems with both model uncertainty and external disturbance. Firstly, we propose a new dynamic feedback control method for complete synchronization of fractional-order nominal systems (without both uncertainty and disturbance). Then, a new uncertainty and disturbance estimator (UDE)-based dynamic feedback control method for the fractional-order systems with both uncertainty and disturbance is presented, by which the synchronization problem of such fractional-order chaotic systems is realized. Finally, the fractional-order Lorenz system is used to demonstrate the practicability of the proposed results.
Chemical and Antioxidant Characteristics of Skin-Derived Collagen Obtained by Acid-Enzymatic Hydrolysis of Bigeye Tuna (Thunnus obesus)
Authors: Devita Liza , Nurilmala Mala , Lioe Hanifah Nuryani , Suhartono Maggy T.
Published: April 2021

The utilization of bigeye tuna skin as a source of collagen has been increasing the value of these skins. In this study, the quality of the skin was studied first. The skin after 14 h freeze-drying showed a high protein level (65.42% ± 0.06%, db), no histamine and a lack of heavy metals. The collagens were extracted through acid and acid-enzymatic methods. The enzymes used were bromelain, papain, pepsin, and trypsin. The two highest-yield collagens were pepsin-soluble collagen (PSC) and bromelain-soluble collagen (BSC). Both were type I collagen, based on SDS-PAGE and FTIR analysis. They dissolved very well in dimethyl sulfoxide and distilled water. The pH ranges were 4.60–4.70 and 4.30–4.40 for PSC and BSC, respectively. PSC and BSC were free from As, Cd, Co, Cr, Cu, and Pb. They showed antioxidant activities, as determined by the DPPH method and the reducing power method. In conclusion, bigeye tuna skin shows good potential as an alternative source of mammalian collagen. Although further work is still required, PSC and BSC showed the potential to be further used as antioxidant compounds in food applications. Other biological tests of these collagens might also lead to other health applications.