bluuebunny/crossref_metadata_2025 huggingface dataset:
ALX-Related Frontonasal Dysplasias: Clinical Characteristics and Surgical Management
Authors: Vargel Ibrahim , Canter Halil Ibrahim , Kucukguven Arda , Aydin Asim , Ozgur Figen
Published: June 2021

Aim:

The term frontonasal dysplasia (FND) represents a spectrum of anomalies and its genetics have not been well defined. Recently, the critical role of the aristaless-like homeobox ( ALX) gene family on the craniofacial development has been discovered. In the present study, we aimed to propose a systematic surgical treatment plan for the ALX-related FNDs according to the genotypic classification as well as demonstrating their clinical characteristics to help surgeons diagnose the underlying pathology accurately.

Design:

Single-institution retrospective.

Setting:

Tertiary health care.

Patients and Methods:

Eighty-nine FND cases were evaluated. Eight of them had ALX1-related FND3, 3 had ALX3-related FND1, and 2 had ALX4-related FND2. Phenotype characteristics of ALX-related FNDs were evaluated, and relevant surgical interventions were assessed.

Results:

The ALX1-related FND3 phenotype is striking due to the involvement of the eyes in addition to the presence of hypertelorism, facial clefts, and nasal deformities. A widened philtrum and prominent philtral columns are remarkable features of the ALX3-related FND1, whereas the ALX4-related FND2 has more severe deformities: severe hypertelorism, brachycephaly, large parietal bone defects, broad nasal dorsum, and alopecia. Facial bipartition, box osteotomies, eyelid coloboma repair, cleft lip and palate repair, nasal reconstruction, and fronto-orbital advancement can be performed in ALX-related FNDs based on the characteristics of each subtype.

Conclusions:

This genetic classification system will help surgeon diagnose patients with FND with unique features and draw a roadmap for their treatment with a better surgical perspective.
Mild gray matter atrophy in patients with long-standing multiple sclerosis and favorable clinical course
Authors: Cortese Rosa , Battaglini Marco , Parodi Francesca , Stromillo Maria Laura , Portaccio Emilio , Razzolini Lorenzo , Giorgio Antonio , Sormani Maria Pia , Amato Maria Pia , De Stefano Nicola
Published: June 2021

The mechanisms responsible for the favorable clinical course in multiple sclerosis (MS) remain unclear. In this longitudinal study, we assessed whether magnetic resonance imaging (MRI)-based changes in focal and diffuse brain damage are associated with a long-term favorable MS diseases course. We found that global brain and gray matter (GM) atrophy changes were milder in MS patients with long-standing disease (⩾30 years from onset) and favorable (no/minimal disability) clinical course than in sex-age-matched disable MS patients, independently of lesions accumulation. Data showed that different trajectories of volume changes, as reflected by mild GM atrophy, may characterize patients with long-term favorable evolution.
The Relationship Between Dynamic Balance Ability and Shoulder Pain in High School Baseball Pitchers
Authors: Nakase Kurumi , Shitara Hitoshi , Tajika Tsuyoshi , Kuboi Takuro , Ichinose Tsuyoshi , Sasaki Tsuyoshi , Hamano Noritaka , Endo Fumitaka , Kamiyama Masataka , Miyamoto Ryosuke , Yamamoto Atsushi , Kobayashi Tsutomu , Takagishi Kenji , Chikuda Hirotaka
Published: June 2021

Background:

Dynamic balance is essential for pitching motion because pitching kinematics requires whole body coordination. The Star Excursion Balance Test (SEBT) and the Y balance test (YBT) evaluate dynamic balance quantitatively. There are some reports that investigated the relationship between SEBT/YBT and pain in upper and lower extremities, but there is no study among high school baseball pitchers.

Hypothesis:

Dynamic balance deficiency is associated with shoulder pain among high school baseball pitchers.

Study Design:

Cross-sectional study.

Level of Evidence:

Level 4.

Methods:

A total of 259 male high school pitchers who participated in the preseason medical checkups were included in the study. YBT was used to measure their dynamic balance. The participants completed a questionnaire which asked if they were currently experiencing shoulder pain.

Results:

Twenty-two pitchers had shoulder pain during the preseason medical checkups. In the YBT, the posterolateral balance while standing with the axis leg as well as the posteromedial and posterolateral balance while standing with the step leg were significantly lower in the pain group than in the nonpain group ( P = 0.05, 0.04, and 0.001, respectively). A logistic regression analysis showed that posterolateral balance when standing with the step leg was an independent risk factor for current shoulder pain ( P = 0.04, odds ratio 0.942, 95% CI 0.892-0.996).

Conclusion:

The dynamic balance of high school baseball pitchers with shoulder pain was lower than that of participants without shoulder pain. In particular, posterolateral direction with the step leg standing was significantly related to shoulder pain.

Clinical Relevance:

Among high school baseball pitchers, decreased dynamic balance was related to current shoulder pain. YBT maybe recommended in preseason medical checkups for high school baseball pitchers.
Emerging Adults’ Self-Efficacy as a Resource for Coping With the COVID-19 Pandemic
Authors: Recksiedler Claudia , Landberg Monique
Published: June 2021

The COVID-19 pandemic is impacting emerging adults during a crucial developmental period, which may have long-lasting effects on their developmental task progression and psychosocial adjustment. Because self-efficacy is a well-researched psychological resource to deal with substantial challenges, the present study examined the link between emerging adults’ life- and domain-specific satisfaction during the pandemic and self-efficacy before the pandemic. Drawing on a sample of 377 German emerging adults (56.5% female), we found that self-efficacy prior to the pandemic was not associated with life or domain-specific satisfaction during the pandemic. However, results revealed that associations between domain-specific satisfaction during the pandemic and self-efficacy varied by educational attainment. Results are discussed in light of specific circumstances related to the progression of the pandemic in Germany, possible non-response bias, as well as implications for social policy and future research.
Does Training Parents in Reinforcement Skills or Relationship Skills Enhance Individual Youths’ Cognitive Behavioral Therapy for Anxiety? Outcome, Specificity, and Mediation
Authors: Silverman Wendy K. , Rey Yasmin , Marin Carla E. , Jaccard James , Pettit Jeremy W.
Published: June 2021

We conducted a dismantling design treatment study comparing individual cognitive behavioral therapy (CBT), CBT targeting parents’ reinforcement skills (CBT + Reinf), and CBT targeting parents’ relationship skills (CBT+ Relat) in 341 youths with primary anxiety diagnoses. At posttreatment, youths in CBT with parent involvement had lower anxiety than youths in CBT. At 12-month follow-up, youths in CBT + Relat maintained lower anxiety relative to CBT. At posttreatment, negative reinforcement was significantly lower in CBT + Reinf than CBT + Relat and CBT; negative reinforcement partially mediated anxiety reduction in youths. Reducing parental negative reinforcement in CBT + Reinf was associated with lower parental psychological control, which also partially mediated anxiety reduction in youths. Some of these mediational dynamics continued through follow-up. Targeting concrete behavioral parenting skills, especially negative reinforcement, produced treatment specificity and partial mediation relative to less concrete targeting and enhanced CBT. Findings highlight complexities in identifying mechanisms through which targeting of parenting skills produces anxiety reduction in youths and suggest avenues for future research.
Hyaluronidase for Treating Complications Related to HA Fillers: A National Plastic Surgeon Survey
Authors: Olaiya Oluwatobi R. , Forbes Diana , Humphrey Shannon , Beleznay Katie , Mosher Mathew , Carruthers Jean
Published: June 2021

Background: Hyaluronic acid (HA) fillers have become a popular modality to address changes in the ageing face. There are many described indications of hyaluronidases in aesthetic medicine which include their use in the management of HA-associated complications. To better understand the current practice patterns, we surveyed Canadian plastic surgeons on their use of hyaluronidases. Methods: With the approval of the Canadian Society of Plastic Surgeons, an electronic survey was emailed to members. A total of 350 surveys were distributed and 98 surveys were completed for a response rate of 28%. Results: Approximately half (48%) of the survey respondents used HA fillers in their practice. Skin testing for hypersensitivity reactions was performed by less than 10% of hyaluronidase users. Nearly all respondents used hyaluronidase for filler over-correction (95.5%) and asymmetry (86.4%). Over half of the respondents have used hyaluronidase for inflammatory or infectious nodules and the Tyndall effect. Other reported applications included restoration of vascular compromise, and one respondent reported using hyaluronidase for assisting with haematoma resolution. When compared with the most recent guidelines, there was a wide range of doses used for common side effects and complications. Twenty-four percent of the respondents reported that their hyaluronidase formulation was prepared by a compounding pharmacy, and 20% of respondents who inject HA fillers did not stock hyaluronidase. Conclusion: There are many indications for hyaluronidase in aesthetic plastic surgery. Plastic surgeons should stock hyaluronidase and develop a specific plan in anticipation of adverse events. Although hyaluronidase is commonly used by plastic surgeons for over-correction and asymmetry, the dosages used in aesthetic practice is rather diverse and heterogeneous. When possible, plastic surgeons should perform allergy testing before hyaluronidase use.
Nitric oxide in occurrence, progress and therapy of lung Cancer: a systemic review and meta-analysis
Authors: Zhou Hongbin , Li Jiuke , Chen Zhewen , Chen Ying , Ye Sa
Published: June 2021

Background

Nitric oxide (NO) plays an important role in lung cancer. However, the results of previous studies about NO in the occurrence, progress and therapy were not consistent. Therefore, we conducted a meta-analysis to evaluate the relationship between NO and lung cancer.

Method

We carried out comprehensive search in the databases, and collected related studies. The data of fraction of exhaled nitric oxide (FeNO) or blood NO in different populations (lung cancer patients and control subjects) and different time points (before therapy and after therapy) were extracted by two investigators. A random effect model was applied to analyze the differences of FeNO and blood NO in different populations and different time points. To further compare NO level of each subgroup with different pathological types and different stages, a network meta-analysis (NMA) was performed.

Results

Fifty studies including 2551 cases and 1691 controls were adopted in this meta-analysis. The FeNO (SMD 3.01, 95% CI 1.89–4.13, p < 0.00001) and blood NO (SMD 1.34, 95% CI 0.84–1.85, p < 0.00001) level in lung cancer patients was much higher than that in control subjects. NMA model indicated blood NO level in each cancer type except SCLC was higher than that in control patients. There was no significant difference of blood NO level among four kinds of lung cancer patients. Blood NO level in LCC patients (SUCRA = 83.5%) was the highest. Blood NO level in advanced stage but not early stage was higher than that in control subjects. Patients in advanced stage (SUCRA = 95.5%) had the highest blood NO level. No significant difference of FeNO (SMD -0.04, 95% CI -0.46-0.38, p > 0.05) and blood NO level (SMD -0.36, 95% CI -1.08-0.36, p > 0.05) was observed between pretreatment and posttreatment in all patients. However, FeNO level elevated (SMD 0.28, 95% CI 0.04–0.51, p = 0.02) and blood NO level decreased in NSCLC patients (SMD -0.95, 95% CI -1.89-0.00, p = 0.05) after therapy.

Conclusion

FeNO and blood NO level would contribute to diagnosis of lung cancer and evaluation of therapy effect, especially for NSCLC patients.
The role of chicken management practices in children’s exposure to environmental contamination: a mixed-methods analysis
Authors: Passarelli Simone , Ambikapathi Ramya , Gunaratna Nilupa S. , Madzorera Isabel , Canavan Chelsey R. , Noor Ramadhani Abdallah , Tewahido Dagmawit , Berhane Yemane , Sibanda Simbarashe , Sibanda Lindiwe Majele , Madzivhandila Tshilidzi , Munthali Bertha L. M. , McConnell Margaret , Sudfeld Christopher , Davison Kirsten , Fawzi Wafaie
Published: June 2021

Background

Household chicken production presents an opportunity to promote child nutrition, but the benefits might be offset by increased environmental contamination. Using household surveys, direct observations, and in-depth interviews with woman caregivers, we sought to describe the relationship between chicken management practices and household exposure to environmental contamination, and assess barriers to adopting improved husbandry practices.

Methods

First, we analyzed baseline data from 973 households raising chickens in the two interventions arms from the Agriculture-to-Nutrition (ATONU) study in Ethiopia to assess the relationship between animal management practices and environmental exposures. Second, we conducted six-hour direct observations of children’s environmental exposures in 18 households. Among these households, we analyzed in-depth interviews with child caregivers.

Results

Quantitative analyses showed that households raised approximately 11 chickens, had animal feces visible on the property 67% of the time, and children’s hands were visibly dirty 38% of the time. Households with more chickens had lower exposure to animal feces. Having a chicken coop increased the risk of observing animal feces on the property by 30%, but among those with a coop, having an enclosed coop reduced that risk by 83%. Coops that were enclosed, had fencing, and were located further from homes were associated with a reduced risk of observing animal feces and an increased likelihood of children having clean hands. Direct observations showed that chicken coops were often poorly designed or not used. On average, 3 to 5 chickens were inside homes at a time, and livestock and domestic animals were frequently inside of houses and interacting with young children. In-depth interviews revealed that protection of animals, maintenance of household cleanliness and health, type of chicken (local versus improved) and resource constraints influenced management decisions.

Conclusions

Improvements in chicken management practices could mitigate the exposure of household members to environmental contamination. Our findings highlight the need for training and resources to promote safe animal husbandry practices and optimal child health in nutrition-sensitive livestock projects.

Trial registration

Clinical trials number: NCT03152227; Retrospectively registered at ClinicalTrials.gov on May 12, 2012.
Atypical oral candidiasis in a psoriatic patient during targeted immunotherapy with an interleukin 17 inhibitor (secukinumab)
Authors: Picciani Bruna Lavinas Sayed , Dziedzic Arkadiusz , Werneck Juliana Tristão , Marinho Marcello Alves , Dick Thaylla Núñez Amin , Quintanilha Nara Regina , Dias Eliane Pedra
Published: June 2021

Background

Secukinumab is a human monoclonal antibody immunoglobulin that neutralises interleukin (IL)-17A, and as such, is effective in the treatment of psoriasis. However, as IL-17A is essential in protection against fungal infections, patients treated with this drug may develop candidiasis. This report presents a case of atypical oral candidiasis occurring during targeted drug immunotherapy with an interleukin 17 (IL-17) inhibitor (secukinumab), with the aim of emphasisinge the necessity of periodical oral health assessment and monitoring. It provides a rational clinical approach to therapeutic protocol in the treatment of side effects associated with novel medications for autoimmune diseases.

Case presentation

Symptomatic tongue lesions were observed in a 50-year-old female patient on a monthly systemic treatment of 300 mg of secukinumab, which appeared after 60 days of using the medication. Two inconclusive biopsies and an unsuccessful application of oral corticosteroids made the diagnostic process challenging. Papillae on the back of the tongue were atrophied, forming a well-defined erythema and white non-detachable plaques on the lateral border of the tongue. Cytopathological and histopathological exam results were compatible with a diagnosis of oral candidiasis. Topical antifungal medication led to subsequent regression of the tongue lesions. During asymptomatic period and follow up for 7 months, a reduced monthly dose 150 mg of secukinumab was administered.

Conclusions

Patients undergoing treatment with IL-17 blockers, such as secukinumab, should be carefully monitored in order to avoid oral side effects resulting from the use of this medication.
Impacts of innovation in dental care delivery and payment in Medicaid managed care for children and adolescents
Authors: Conrad Douglas A. , Milgrom Peter , Du Yuxian , Cunha-Cruz Joana , Ludwig Sharity , Shirtcliff R. Mike
Published: June 2021

Background

We evaluated a 14-county quality improvement program of care delivery and payment of a dental care organization for child and adolescent managed care Medicaid beneficiaries after 2 years of implementation.

Methods

Counties were randomly assigned to either the intervention (PREDICT) or control group. Using Medicaid administrative data, difference-in-difference regression models were used to estimate PREDICT intervention effects (formally, “average marginal effects”) on dental care utilization and costs to Medicaid, controlling for patient and county characteristics.

Results

Average marginal effects of PREDICT on expected use and expected cost of services per patient (child or adolescent) per quarter were small and insignificant for most service categories. There were statistically significant effects of PREDICT (p < .05), though still small, for certain types of service:

Expected number of diagnostic services per patient-quarter increased by .009 units;

Expected number of sealants per patient-quarter increased by .003 units, and expected cost by $0.06;

Total expected cost per patient-quarter for all services increased by $0.64.

These consistent positive effects of PREDICT on diagnostic and certain preventive services (i.e., sealants) were not accompanied by increases in more costly service types (i.e., restorations) or extractions.

Conclusion

The major hypothesis that primary dental care (selected preventive services and diagnostic services in general) would increase significantly over time in PREDICT counties relative to controls was supported. There were small but statistically significant, increases in differential use of diagnostic services and sealants. Total cost per beneficiary rose modestly, but restorative and dental costs did not. The findings suggest favorable developments within PREDICT counties in enhanced preventive and diagnostic procedures, while holding the line on expensive restorative and extraction procedures.
Acceptability of single screening and treatment policy for the control of malaria in pregnancy: perceptions of providers and pregnant women from selected health facilities in Lindi region, Tanzania
Authors: Kitojo Chonge , Chacky Frank , Kigadye Emmanuel S. , Mugasa Joseph P. , Lusasi Abdallah , Mohamed Ally , Reaves Erik J. , Gutman Julie R. , Ishengoma Deus S.
Published: June 2021

Background

Tanzania started implementing single screening and treatment (SST) for all pregnant women attending their first antenatal care (ANC) visits in 2014, using malaria rapid diagnostic tests (RDTs) and treating those who test positive according to the national guidelines. However, there is a paucity of data to show the acceptability of SST to both pregnant women and health care workers (HCWs), taking into consideration the shortage of workers and the added burden of this policy to the health system. This study assessed the perceptions and opinions of health service users and providers to determine the acceptability of SST policy.

Methods

Pregnant women and HCWs in eight health facilities in two districts of Lindi region (Kilwa and Lindi) were interviewed using semi-structured questionnaires with open and close-ended questions. Both qualitative and quantitative data were collected, including demographic characteristics, women’s experience, their perception on SST and challenges they face when receiving services for malaria offered at ANC. Experience of HCWs regarding the implementation of SST as part of routine services and the challenges encountered when providing ANC services for malaria in pregnancy (MIP) were also assessed.

Results

Of the 143 pregnant women interviewed, 97% viewed testing favourably and would wish to be tested for malaria again, while 95% were satisfied with services and reasons for testing during the first ANC visit. Nearly all (99%) would recommend their fellow pregnant women to be tested for malaria and all women recommended that the Ministry of Health should continue the SST strategy. This was despite the fact that 76% of the women experienced pain and 16% had anxiety as a result of finger prick. Sixteen HCWs (mostly nurses) were interviewed; they also viewed SST implementation favourably and reported feeling empowered to use RDTs for malaria screening. The main challenge identified by HCWs was that nurses are not allowed to prescribe anti-malarials to women who test positive and need to refer them to the outpatient department for treatment.

Conclusion

SST was considered an acceptable approach to control MIP by HCWs and pregnant women, and they recommended the continuation of the policy. In addition, consideration should be given to implementing a task-shifting policy to allow nurses to dispense anti-malarials to pregnant women.
Conspecific odor cues induce different vocal responses in serrate-legged small treefrogs, but only in the absence of acoustic signals
Authors: Deng Ke , Zhou Ya , He Qiao-Ling , Zhu Bi-Cheng , Wang Tong-Liang , Wang Ji-Chao , Cui Jian-Guo
Published: June 2021

Background

Signal detection is crucial to survival and successful reproduction, and animals often modify behavioral decisions based on information they obtained from the social context. Undeniably, the decision-making in male-male competition and female choice of anurans (frogs and toads) depends heavily on acoustic signals. However, increasing empirical evidence suggests that additional or alternative types of cue (e.g., visual, chemical, and vibratory) can be used to detect, discriminate and locate conspecifics in many anuran species. Nevertheless, few studies have investigated whether conspecific odor cues affect male’s calling behavior. In this study, we conducted an experiment to investigate whether and how different chemical cues (male odors, female odors, and stress odors) from conspecifics affect male’s calling strategies in serrate-legged small treefrogs (Kurixalus odontotarsus), and whether the combined chemical and acoustic stimuli have additive effects on calling behavior or not.

Results

We found that compared with female odors, male K. odontotarsus reduced calling investment in response to male odors or stress odors, in the absence of rival’s advertisement calls. When odor stimuli and advertisement calls were presented simultaneously, however, there were no differences in the vocal response of focal males among odor groups.

Conclusions

These results provide evidence that male treefrogs switch calling investment according to different odor cues from conspecifics, and further demonstrate that calling behavior can be affected by chemical cues in anuran species. Our study highlights the potential role of airborne chemical cues in sex identification and contributes to increase our understanding of anuran communication.
The relationship between family intimacy and relapse tendency among people who use drugs: a moderated mediation model
Authors: Zeng Xiaoqing , Lu Mengyao , Chen Meirong
Published: June 2021

Background

Drug addiction is difficult to overcome. The relapse rate is high, and the negative impact on individuals, families and society is severe, therefore exploring social psychological mechanisms to reduce relapse has very important theoretical and practical value. However, the underlying mechanism by which the interaction between family and individual factors influences the tendency to relapse remain unclear. Thus, the purpose of this paper is to discuss the relationship between family intimacy and relapse tendency of people who use drugs, as well as the mediating effect of psychological capital and the role of self-efficacy in it.

Methods

A total of 817 male who use drugs were investigated via the Family Intimacy and Adaptability Scale, General Self-Efficacy Scale, Positive Psychological Capital Questionnaire and Relapse Tendency Questionnaire. Using Hayes’s process macro carried out moderated mediation analysis.

Results

(1) The average family intimacy score of people who use drugs was low. (2) Family intimacy negatively predicted relapse tendency in people who use drugs. (3) Psychological capital mediated the relationship between family intimacy and relapse tendency. (4) The first half of the indirect effect of family intimacy on relapse tendency was regulated by self-efficacy, compared with the low level of self-efficacy, the psychological capital level with high self-efficacy is higher.

Conclusion

The results of this study suggest that the intimacy between the people who use drugs and their family members should be improved, and the rehabilitation center should take various measures to enhance the psychological capital level and the level of self-efficacy of the people who use drugs, which will be helpful to reduce their relapse tendency.
BUTTERFLY: addressing the pooled amplification paradox with unique molecular identifiers in single-cell RNA-seq
Authors: Gustafsson Johan , Robinson Jonathan , Nielsen Jens , Pachter Lior
Published: June 2021

The incorporation of unique molecular identifiers (UMIs) in single-cell RNA-seq assays makes possible the identification of duplicated molecules, thereby facilitating the counting of distinct molecules from sequenced reads. However, we show that the naïve removal of duplicates can lead to a bias due to a “pooled amplification paradox,” and we propose an improved quantification method based on unseen species modeling. Our correction called BUTTERFLY uses a zero truncated negative binomial estimator implemented in the kallisto bustools workflow. We demonstrate its efficacy across cell types and genes and show that in some cases it can invert the relative abundance of genes.
Unbiased profiling of CRISPR RNA-guided transposition products by long-read sequencing
Authors: Vo Phuc Leo H. , Acree Christopher , Smith Melissa L. , Sternberg Samuel H.
Published: June 2021

Bacterial transposons propagate through either non-replicative (cut-and-paste) or replicative (copy-and-paste) pathways, depending on how the mobile element is excised from its donor source. In the well-characterized E. coli transposon Tn7, a heteromeric TnsA-TnsB transposase directs cut-and-paste transposition by cleaving both strands at each transposon end during the excision step. Whether a similar pathway is involved for RNA-guided transposons, in which CRISPR-Cas systems confer DNA target specificity, has not been determined. Here, we apply long-read, population-based whole-genome sequencing (WGS) to unambiguously resolve transposition products for two evolutionarily distinct transposon types that employ either Cascade or Cas12k for RNA-guided DNA integration. Our results show that RNA-guided transposon systems lacking functional TnsA primarily undergo copy-and-paste transposition, generating cointegrate products that comprise duplicated transposon copies and genomic insertion of the vector backbone. Finally, we report natural and engineered transposon variants encoding a TnsAB fusion protein, revealing a novel strategy for achieving RNA-guided transposition with fewer molecular components.
Linking sports registration information and player feedback to enhance netball participation
Authors: Foley Bridget C. , Rose Catriona , Owen Katherine B. , Reece Lindsey J.
Published: June 2021

Background

Sports should endeavour to provide inclusive opportunities for all people to participate. More evidence is required to understand who joins sports clubs and what keeps participants engaged throughout their lifetime. Little is known about who plays netball or what drives participation and retention of players in netball. This study aimed to identify the sociodemographic characteristics of Netball New South Wales (NSW) members, their odds of re-registering in the sport, and explore their motivations to play, experiences during participation and the perceived benefits of playing netball.

Methods

We used longitudinal sport registration data from all Netball NSW members in 2018 and 2019. A cross-sectional online survey was sent to all players registered during the two-year study period (n = 157,152). We used logistic regression to determine the odds of individuals returning to netball in 2019 after playing in 2018 and linked the sports registration data with the survey responses, calculating frequencies and proportions.

Results

Netball NSW members were mostly female (98 %) and aged less than 18 years old (69 %). Netball NSW retained 68 % of members in 2019 who played the previous year. Members who were male, aged 18–44 years old, lived in low SES areas, lived in regional/remote locations, identified as Aboriginal or Torres Strait Islander, spoke a language other than English at home or were born outside Australia had lower odds of returning to play from 2018 to 2019. Survey participants (n = 10,795) rated their experience playing netball highly and reported that playing netball improved their health and wellbeing. The main reason to play netball reported was ‘fun and enjoyment’ while the main reason to consider quitting was the ‘skill/experience of umpires and officials’.

Conclusions

This study highlights the strengths of netball in engaging and retaining females, who often participate in less sport than males. The positive experiences reported by netball participants should be fostered to retain current participants throughout the lifecourse. The data provided by members should be inform strategic actions to enhance netball participation for sociodemographic groups who had greatest odds of dropout. Routine surveillance using linked registration and player feedback should be utilised by sports to enhance sport delivery and increase participation.
Visual assessment of movement quality: a study on intra- and interrater reliability of a multi-segmental single leg squat test
Authors: Ressman John , Grooten Wilhelmus Johannes Andreas , Rasmussen-Barr Eva
Published: June 2021

Background

The Single Leg Squat test (SLS) is a common tool used in clinical examination to set and evaluate rehabilitation goals, but there is not one established SLS test used in the clinic. Based on previous scientific findings on the reliability of the SLS test and with a methodological rigorous setup, the aim of the present study was to investigate the intra- and interrater reliability of a standardised multi-segmental SLS test.

Methods

We performed a study of measurement properties to investigate the intra- and interrater reliability of a standardised multi-segmental SLS test including the assessment of the foot, knee, pelvis, and trunk. Novice and experienced physiotherapists rated 65 video recorded SLS tests from 34 test persons. We followed the Quality Appraisal for Reliability Studies checklist.

Results

Regardless of the raters experience, the interrater reliability varied between “moderate” for the knee variable (ĸ = 0.41, 95% CI 0.10–0.72) and “almost perfect” for the foot (ĸ = 1.00, 95% CI 1.00–1.00). The intrarater reliability varied between “slight” (pelvic variable; ĸ = 0.17, 95% CI -0.22-0.55) to “almost perfect” (foot variable; ĸ = 1.00, 95% CI 1.00–1.00; trunk variable; ĸ = 0.82, 95% CI 0.66–0.97). A generalised kappa coefficient including the values from all raters and segments reached “moderate” interrater reliability (ĸ = 0.52, 95% CI 0.43–0.61), the corresponding value for the intrarater reliability reached “almost perfect” (ĸ = 0.82, 95% CI 0.77–0.86).

Conclusions

The present study shows a “moderate” interrater reliability and an “almost perfect” intrarater reliability for the variable all segments regardless of the raters experience. Thus, we conclude that the proposed standardised multi-segmental SLS test is reliable enough to be used in an active population.
The impacts of land-use and land-cover change on wetland ecosystem service values in peri-urban and urban area of Bahir Dar City, Upper Blue Nile Basin, Northwestern Ethiopia
Authors: Assefa Workiyie Worie , Eneyew Belachew Getnet , Wondie Ayalew
Published: June 2021

Background

Even though wetlands are essential in enhancing water quality and providing recreation and entertainment opportunities in urban areas, their values are overlooked by the decision-makers. Underestimation of the economic value of wetland ecosystem services contributes to their continuing deterioration and inevitable loss. Investigating the changes in ecosystem service values (ESV) can provide crucial information for decision-making. This study, therefore, analyzes the temporal and spatial land-use/land-cover (LULC) change patterns over 35 years (1984–2019) intending to evaluate its impact on wetland ecosystem service values in Bahir Dar City, Ethiopia. Estimation and change analyses of ESVs were conducted by employing ArcGIS using LULC inputs of the year 1984, 1994, 2004, 2014, and 2019 with their corresponding global value coefficients that were developed earlier and our own modified value coefficients for the studied landscape.

Results

The results showed that wetlands and water bodies of the city and its peripheries had decreased by 75.71% (−1618 ha) within 35 years, while built-up area increased by 216.24% (+2599 ha). Cultivated land had increased slightly from 1984 to 1994 and then gradually declined since 1994. Changes in LULC had resulted in a decline of the total ESV. The total ESV had decreased from USD 29.73 × 106 to USD 20.84 × 106 in 35 years. This indicates the loss of nearly USD 8.9 × 106 ESV from 1984 to 2019. A sensitivity analysis suggested the robustness of ESV estimation in the study area. All individual ecosystem services experienced a negative change. However, a greater reduction in ESV was observed for services such as water regulation, waste treatment, and habitats for maintaining biodiversity. The expansion of built-up area of Bahir Dar City was the major factor that contributed to the loss of ESV provided by wetlands.

Conclusions

The loss of ESV resulting in LULC changes has a negative implication on local climate, waste management, and the livelihoods of the poor community. Thus, interventions should be made for the restoration and sustainable management of wetlands in the urban and peri-urban areas of Bahir Dar City.
Is the use of high correlated color temperature light at night related to delay of sleep timing in university students? A cross-country study in Japan and China
Authors: Higuchi Shigekazu , Lin Yandan , Qiu Jingjing , Zhang Yichi , Ohashi Michihiro , Lee Sang-il , Kitamura Shingo , Yasukouchi Akira
Published: June 2021

Background

Blue-enriched white light at night has the potential to delay the circadian rhythm in daily life. This study was conducted to determine whether the use of high correlated color temperature (CCT) light at home at night is associated with delay of sleep timing in university students.

Methods

The survey was conducted in 2014–2015 in 447 university students in Japan and 327 students in China. Habitual sleep timing and type of CCT light at home were investigated by using a self-administered questionnaire. The Japanese students were significantly later than the Chinese students in bedtime, wake time, and midpoint of sleep. They were asked whether the lighting in the room where they spend most of their time at night was closer to warm color (low CCT) or daylight color (high CCT). The amount of light exposure level during daily life was measured for at least 1 week by the use of a light sensor in 60 students in each country.

Results

The percentages of participants who used high CCT lighting at night were 61.6% for Japanese students and 80.8% for Chinese students. Bedtime and sleep onset time on school days and free days were significantly later in the high CCT group than in the low CCT group in Japan. The midpoint of sleep in the high CCT group was significantly later than that in the low CCT group on free days but not on school days. On the other hand, none of the sleep measurements on school days and free days were significantly different between the high CCT and low CCT groups in China. Illuminance level of light exposure during the night was significantly higher in Japanese than in Chinese, but that in the morning was significantly higher in China than in Japan.

Conclusions

The use of high CCT light at night is associated with delay of sleep timing in Japanese university students but not in Chinese university students. The effects of light at night on sleep timing and circadian rhythm may be complicated by other lifestyle factors depending on the country.
SARS-CoV-2 detection status associates with bacterial community composition in patients and the hospital environment
Authors: Marotz Clarisse , Belda-Ferre Pedro , Ali Farhana , Das Promi , Huang Shi , Cantrell Kalen , Jiang Lingjing , Martino Cameron , Diner Rachel E. , Rahman Gibraan , McDonald Daniel , Armstrong George , Kodera Sho , Donato Sonya , Ecklu-Mensah Gertrude , Gottel Neil , Salas Garcia Mariana C. , Chiang Leslie Y. , Salido Rodolfo A. , Shaffer Justin P. , Bryant Mac Kenzie , Sanders Karenina , Humphrey Greg , Ackermann Gail , Haiminen Niina , Beck Kristen L. , Kim Ho-Cheol , Carrieri Anna Paola , Parida Laxmi , Vázquez-Baeza Yoshiki , Torriani Francesca J. , Knight Rob , Gilbert Jack , Sweeney Daniel A. , Allard Sarah M.
Published: June 2021

Background

SARS-CoV-2 is an RNA virus responsible for the coronavirus disease 2019 (COVID-19) pandemic. Viruses exist in complex microbial environments, and recent studies have revealed both synergistic and antagonistic effects of specific bacterial taxa on viral prevalence and infectivity. We set out to test whether specific bacterial communities predict SARS-CoV-2 occurrence in a hospital setting.

Methods

We collected 972 samples from hospitalized patients with COVID-19, their health care providers, and hospital surfaces before, during, and after admission. We screened for SARS-CoV-2 using RT-qPCR, characterized microbial communities using 16S rRNA gene amplicon sequencing, and used these bacterial profiles to classify SARS-CoV-2 RNA detection with a random forest model.

Results

Sixteen percent of surfaces from COVID-19 patient rooms had detectable SARS-CoV-2 RNA, although infectivity was not assessed. The highest prevalence was in floor samples next to patient beds (39%) and directly outside their rooms (29%). Although bed rail samples more closely resembled the patient microbiome compared to floor samples, SARS-CoV-2 RNA was detected less often in bed rail samples (11%). SARS-CoV-2 positive samples had higher bacterial phylogenetic diversity in both human and surface samples and higher biomass in floor samples. 16S microbial community profiles enabled high classifier accuracy for SARS-CoV-2 status in not only nares, but also forehead, stool, and floor samples. Across these distinct microbial profiles, a single amplicon sequence variant from the genus Rothia strongly predicted SARS-CoV-2 presence across sample types, with greater prevalence in positive surface and human samples, even when compared to samples from patients in other intensive care units prior to the COVID-19 pandemic.

Conclusions

These results contextualize the vast diversity of microbial niches where SARS-CoV-2 RNA is detected and identify specific bacterial taxa that associate with the viral RNA prevalence both in the host and hospital environment.
Impact of COVID-19 on the mental health of US college students
Authors: Lee Jenny , Solomon Matthew , Stead Tej , Kwon Bryan , Ganti Latha
Published: June 2021

Background/aim

In the beginning of 2020, the novel Coronavirus disease (COVID-19) caused by the SARS-CoV-2 virus, became a public health emergency in the U.S. and rapidly escalated into a global pandemic. Because the SARS-CoV-2 virus is highly contagious, physical distancing was enforced and indoor public spaces, including schools and educational institutions, were abruptly closed and evacuated to ensure civilian safety. Accordingly, educational institutions rapidly transitioned to remote learning. We investigated the impacts of the COVID-19 pandemic on domestic U.S. college students, ages 18–24 years.

Methods

Through Pollfish®’s survey research platform, we collected data from 200 domestic U.S. college students in this age range (N = 200) regarding the physical, emotional, and social impacts of COVID-19 as well as key background information (e.g. whether or not they are first-generation or if they identify with the LGBTQIA+ community).

Results

Our results indicate that students closer to graduating faced increases in anxiety (60.8%), feeling of loneliness (54.1%), and depression (59.8%). Many reported worries for the health of loved ones most impacted their mental health status (20.0%), and the need to take care of family most affected current and future plans (31.8%). Almost one-half of students took to exercising and physical activity to take care of their mental health (46.7%). While a third did not have strained familial relationships (36.5%), almost one half did (45.7%). A majority found it harder to complete the semester at home (60.9%), especially among those who had strained relationships with family (34.1%). Seventy percent spent time during the pandemic watching television shows or movies. Significantly more men, first-generation, and low-income students gained beneficial opportunities in light of the pandemic, whereas their counterparts reported no impact. First-generation students were more likely to take a gap year or time off from school.

Conclusions

Although students found ways to take care of themselves and spent more time at home, the clear negative mental health impacts call for schools and federal regulations to accommodate, support, and make mental health care accessible to all students.
Biorefinery methods for extraction of oil and protein from rubber seed
Authors: Yang Miao , Zhu Wenlei , Cao Hui
Published: June 2021

Rubber seeds are a by-product of rubber production and are rich in oil and protein. Upgrading of rubber seeds to produce proteins, oils and feedstock can generate additional revenue for rubber production and reduce waste. The present study investigates the effects of different pre-treatments and extraction methods to determine the optimal methods to produce oil and protein from rubber seed kernels. Mechanical expulsion using a screw press and solvent extraction using n-hexane were employed for oil separation. The highest oil recovery efficiency of 95.12% was obtained using rubber seed meal that was pre-dried at 105 ℃. The sequential water–alkaline treatment was ideal for achieving high protein recovery while reducing the protein denaturation that can result from high operating temperatures and organic solvent contact. Over 90% of the total protein from rubber seed kernels could be recovered. Separating oil from kernels using hexane followed by protein extraction from the meals by enzymatic treatment provides a suitable method for comprehensive utilization of rubber seeds.
Evaluation of the bioformulation of potent native strains of Trichoderma spp. against the foot rot/gummosis of Kinnow mandarin
Authors: Choudhary Ajay Kumar , Singh Narinder , Singh Daljeet
Published: June 2021

Background

Foot rot/gummosis of citrus, caused by Phytophthora spp., is the most important disease of citrus, responsible for the yield losses of 10 to 30% in fruit production in major citrus growing countries. Considering the burden of disease, the present study was undertaken to evaluate different native potent strains of Trichoderma spp. against the foot rot/gummosis of citrus.

Results

Based on molecular characteristics, i.e., ITS ribosomal DNA sequence analysis, 3 isolates of Phytophthora nicotianae var. parasitica and 7 isolates of Trichoderma (6 isolates of T. asperellum and one of T. harzianum) were identified. Trichoderma isolates and Metalaxyl-M + Mancozeb were used to assess antagonistic activity against the foot rot/gummosis under field conditions. T. asperellum T20 mutant reduced gummosis lesion size (95.67%), improved feeder root density (0.0236 cm3), increased number (990.16), and yield of fruits (168.83 kg).

Conclusions

The talc-based bioformulation of T20 mutant (T. asperellum) strain, used as a soil treatment, was able to manage effectively the gummosis/foot rot of citrus and also exhibited the highest yield.
Assessment of production potential of long-linted Gossypium arboreum L. genotypes under different sowing times
Authors: Sankaranarayanan K. , Venugopalan M. V. , Kanjana D.
Published: June 2021

Background

Gossypium arboreum is one of the cultivated diploids which has inherent resistance against insect pests and diseases, can withstand moisture stress, needs less nutrients and is suitable for erratic, less rainfall and marginal soils. Due to its smaller boll size and poor locule retentivity, adoption of species by farmers is very low. So, best long-linted G. arboreum genotypes with high yielding potential and suitable sowing time will be identified for rainfed tracts of cotton cultivated areas in India.

Results

The pooled analysis results found that the significantly highest mean seed cotton yield was recorded with Phule Dhanwanthry (1069 kg/ha) which was on par with K12 (1027 kg/ha), DLSA 17 (977 kg/ha) and PA 812 (951 kg/ha). Planting at 4 August yielded the significantly higher seed cotton yield (1345 kg/ha) in comparison with 4 September sowing (536 kg/ha). The interaction results in pooled data revealed that Genotype PA 812 planted at 4 August registered the significantly highest seed cotton yield (1487 kg/ha) which was on par with all genotypes sown on 4 August except PA 402. Amongst long-linted genotypes, PA 760 recorded the significantly highest upper half length (29.9 mm), mean length (24.9 mm) and fibre strength (27.0 g/tex) and fibre quality index (349.7); which was on par with DLSA 17, PA 812 and PA 402. The quality characters had not been influenced significantly by different times of sowing.

Conclusions

The results concluded that the significantly highest mean seed cotton yield was recorded with Phule Dhanwanthry (1069 kg/ha) which was on par with K12 (1027 kg/ha), DLSA 17 (977 kg/ha) and PA 812 (951 kg/ha). Planting at 4 August found that the significantly higher seed cotton yield (1345 kg/ha) in comparison with 4 September sowing (536 kg/ha). Amongst long-linted genotypes, PA 760 recorded the significantly higher fibre quality index (349.7)
Training Faculty as an Institutional Response to COVID-19 Emergency Remote Teaching Supported by Data
Authors: Walsh Lisa L. , Arango-Caro Sandra , Wester Emma R. , Callis-Duehl Kristine
Published: June 2021

Biology faculty across the United States were surveyed to chronicle their experiences with the COVID-19 emergency transition to remote teaching. Polarizing differences were seen in faculty responses based on previous experience teaching online and formal training received. The results underline the importance of training and highlight difficulties to address.