bluuebunny/crossref_metadata_2025 huggingface dataset:
A Retrospective Study on the Mortality Rate of Camel Calves, Leading Causes, and Associated Risk Factors in Borana Zone, Oromia Regional State, Ethiopia
Authors: Abduljami Gerade , Kumbe Adem
Published: May 2024

Background: Camelus dromedarius (one-humped camel) is an important livestock species uniquely adapted to arid and hot environments. Purpose: This study aims to determine the mortality rate of camel calves and to investigate the potential risk factors associated with it. Methods: Retrospective data were collected through interviews with livestock owners using a semi-structured questionnaire from February 2020 to January 2021. During the study period, a total of 1,249 calves were born. This allowed for assessment of annual mortality rate and its main contributing factors. Results: The average annual mortality rate was 26% (1.29 ±1.77), while the overall mortality rate was 55% (1.30 ± 1.85). Malnutrition (5%), unexplained causes (6%), and diseases (24%) were the three leading causes of calf mortality. Diarrhea (57%), nonspecific diseases (45.4%), respiratory diseases (23%) were significant among the diseases. Mistakes in the management of young livestock included limiting access to colostrum and milk for human consumption, as well as poor care and health management. Conclusion: The results of this study suggested that the pastoral livestock production system used in Borena has a serious problem with calf mortality. The first month of life had the highest mortality rate of 26%. Diseases, starvation, and unexplained causes were the leading causes of calf mortality. In addition, calf mortality was associated with the timing of birth and colostrum feeding.
Isolation and Molecular Identification of Endophytic Fungi Associated with Brown Algae for Inhibiting Escherichia coli ESBL
Authors: Wiradana Putu Angga , Permatasari Anak Agung Ayu Putri , Sari Ni Kadek Yunita , Widhiantara I Gede , Sandhika I Made Gde Sudyadnyana , Waruwu Ernawati , Fimaputra Jatu Maranatha , Panjaitan Novaria Sari Dewi , Putri Fadillaisyia Riandani , Ansori Arif Nur Muhammad
Published: May 2024

Background: The marine environment is the main source of research on natural products in the future. In addition, marine microorganisms have been identified as a natural source capable of developing new antibiotic compounds, including controlling urinary tract infections caused by Escherichia coli ESBL. Purpose: This study aims to isolate, select, and test the potential of brown macroalgae endophytic fungi (Phaeophyceae) collected from the coastal waters of Sanur, Bali Province. Methods: Three types of brown algae were collected from Sanur Beach and their endophytic fungi were isolated using PDA media. Antibacterial activity was determined by measuring the inhibition zone and determining the inhibition category. The selected isolates were cultured and the phytochemical profile was determined qualitatively. In addition, molecular identification using the Internal Transcribed Spacer (ITS) primer set and comparison with the GenBank (NCBI) database were carried out in this study. Results: The results showed that 10 isolates were successfully isolated from three types of brown macroalgae. It was found that isolates T1, S1, and P3 had the highest inhibition in the very strong category. There were variations in mycelial weight, pH value, and inhibition shown by the culture filtrate of the three endophytic fungal isolates against Escherichia coli ESBL. Isolate S1 had the highest phytochemical profile, namely alkaloids, triterpenoids, saponins, and phenolics. The three endophytic fungal isolates showed isolate T1 (Phlebiopsis magnicystidiata) (MT5617191), isolate P3 (Neurospora crassa strain RT3M) (MT1028551), and isolate S1 (Peniophora sp.) (MH2680421). Conclusion: The results of this study provide initial information regarding the potential of bioprospecting brown macroalgal endophytic fungi as a source of new antibiotics against Escherichia coli ESBL.
Anthrax: Distribution, Knowledge, Attitude and Practice Towards Pastoralists in Jawhar, Qalimow and Adan Yabal Somalia.
Authors: Barre Abdirahman , Hassan Abdihamid Salad , Mohamed Abdullahi Nur
Published: May 2024

Anthrax is a Zoonotic Disease (both Human and Animals) the disease is causing higher morbidity and mortality rate in Middle-Shebelle Somalia. Anthrax is a rare infectious disease caused by the bacterium Bacillus anthraces. It was reported in many parts of the region the disease is endemic that effects production with the pastoralists with an incidence >30% of their livestock production system. In a study to determine the status of anthrax in pastoralists in three selected area of Middle-Shebelle: Jawhar, Qalimow, and Aaden yabaal with Forty two herds were also used. The three selected area of the study were the most livestock populations in Middle-Shebelle. A structured Questionnaire was used to capture pastoralists Knowledge, Attitude and Their Practice with regards of Anthrax Disease. A cross-sectional survey was conducted contemporaneously with focus group discussions (FGDs) and key Informant interviews (KIIs) between May 2020 and April 2021. A Four Hundreds (4OO) of Households were visited via Personal interview. The existence of the disease per herd were estimated that 18.1% of camel herds, 34.3% of cattle herds and 47.6% of small ruminants flocks. Thus the summary of the KAP Results indicated that there was lack of Knowledge and Practice Gap among pastoralists related anthrax Disease.  Generally, majority of farmers were practicing poorly in livestock community that was created the distribution of the disease. Therefore, the cattle sheep and goat herds should aware the diseases about the characterization and controlling systems through extending and trading for pastoralists and households. In addition, further assessment and control coverage with anthrax disease will highly option of essentials.
Trypanosoma evansi Infection in Sumba Horses in East Sumba Regency : A Study at BBVet Denpasar
Authors: Wibowo Sarwo Edy , Manin Fahmida , Insulistyowati Anie , Priyatna Fadel Muhammad , Nugraheni Yudhi  Ratna , Baskara Paradewa , Awaludin Aan
Published: May 2024

Background: Sumba is one of the original habitats of the Sandalwood Ponies and the presence of pony is an important element for the community. The Sumbanese herd their ponies in the savanna and rely on nature as a source of horse feed, consequently, during the dry season, the availability of the feed decreases. These conditions can potentially reduce the health status of ponies and increase morbidity or mortality from diseases caused by Trypanosoma evansi. Purpose: This study aims to determine the occurrence and intensity of Trypanosoma evansi infection in blood samples from horses examined at the Denpasar Bali Veterinary Centre. Methods: Thirty blood samples were collected from East Sumba Regency. Three milliliters of blood were taken from the jugular vein and immediately transferred into an ethylenediaminetetraacetic acid tube. Thin blood smears were subsequently prepared and examined using the Giemsa-stained blood smears method. The preparations of blood smear were examined under microscope to determine the Trypanosoma evansi infection and the intensity was calculated on the average number of Trypanosoma evansi in 100 red blood cells. Results: The result showed that six out of 30 horses were tested positive for Trypanosoma evansi. The infection intensity ranged from two to 18 parasites with an average number of 8 in the blood smear examination method. Conclusion: The incidence of trypanosomiasis in Sumba horses varies with each season in East Sumba Regency. The prophylactic measures that should be adopted in the particular herd of horses because this is the most significant blood protozoan parasite of equines.
Amelanotic Choroidal Melanoma with Extraocular Extension in a 51 Year-Old Female: A Rare Case
Authors: Ervianti Octarina , Lutfi Delfitri
Published: May 2024

Introduction: Melanoma is a dangerous malignancy primarily involving the choroid, ciliary body, or iris. The most frequent primary intraocular malignant tumor is choroidal melanoma. The tumor most often affects Caucasians of Northern Europe ranging between the ages of 50 and 80. Most choroidal melanomas are pigmented, however, non-pigmented or mixed pigmented and non-pigmented forms can also happen. Compared with other amelanotic choroidal lesions, amelanotic choroidal melanoma showed markedly greater basal diameter, thickness, frequent connection with subretinal fluid, and ultrasonic hollowness. Extrascleral extension is currently detected in 10% to 28% of choroidal melanoma patients, and the mortality rate is much greater than in those without the extension. Case Presentation: We reported a rare case of extraocular extension in a 51-year-old female patient with amelanotic melanoma. The primary complaint was a painful and bleeding mass on her left eye that had developed two months prior to admission. On the conjunctiva of the left eye, there was a solid, palpable mass measuring 4 x 3.5 x 1 cm. It was red, well-defined, and had a hard, solid consistency. It was fixed at the base, had minimal bleeding, and pressed on her eyeball from the inferolateral direction. The cornea appears clear, and there is no light perception in visual acuity. The orbital focus computed tomography scan revealed an Enhancing solid mass containing a fat component. The mass was in the left intraocular, extending from intraconal to extraconal. Conclusions: Amelanotic melanoma with extraocular extension is a rare condition that can be difficult to detect due to its unclear clinical symptoms and wide range of possible causes. Patients and their families must be educated to receive the appropriate first therapy and prevent the illness from worsening. Melanoma management depends on several factors: tumor size, location, related characteristics, opposite eye status, systemic status, and patient preference. Orbital exenteration is one of the management options for choroidal melanoma with significant extraocular extension. By the time ocular treatment begins, the patient's survival may already be predetermined, and this realization could impact how uveal melanoma is treated in the years to come.
ALK inhibitors in cancer: mechanisms of resistance and therapeutic management strategies
Authors: Poei Darin , Ali Sana , Ye Shirley , Hsu Robert
Published: May 2024

Anaplastic lymphoma kinase (ALK ) gene rearrangements have been identified as potent oncogenic drivers in several malignancies, including non-small cell lung cancer (NSCLC). The discovery of ALK inhibition using a tyrosine kinase inhibitor (TKI) has dramatically improved the outcomes of patients with ALK-mutated NSCLC. However, the emergence of intrinsic and acquired resistance inevitably occurs with ALK TKI use. This review describes the molecular mechanisms of ALK TKI resistance and discusses management strategies to overcome therapeutic resistance.
Calidad de vida en barrios segregados por el Estado ecuatoriano durante el periodo 2008-2018 = Quality of life in segregated Ecuadorian neighborhoods during 2008 - 2018
Authors: Brito Escobar María Lorena , Robayo Tigse Lenin Alejandro
Published: May 2024

ResumenEl presente artículo reflexiona sobre la calidad de vida de la población beneficiaria de vivienda de interés social en Ecuador, producto de la aplicación de políticas habitacionales cuyos contenidos reproducen lógicas de segregación y de discriminación. Con una metodología cuantitativa estructurada en momentos diferenciados (2008 - 2018) y con varios actores, se muestra la frágil sostenibilidad socioeconómica y cultural de los territorios creados por estas intervenciones y su propensión a crear enclaves étnicos, e inclusive, guetos urbanos. Además, se pone en evidencia que las estrategias de acompañamiento social, como herramientas añadidas a dichas políticas, en su intento por mejorar la calidad de vida de la población beneficiaria de vivienda de interés social gratuita, se ven limitadas por realidades contrapuestas a sus metas, como en el caso de los Programas Socio Vivienda 2, en Guayaquil, y Huarcay, en Quito.AbstractThis paper analyses the quality of life of the beneficiary population of social housing programs in Ecuador. It seems housing policies, whose contents reproduce logics of segregation and ethnic discrimination. Within a quantitative methodology structured in differentiated moments and with several actors, it shows the fragile socioeconomic and cultural sustainability of the territories created by this type of intervention. In addition, social support strategies, as tools added to this type of policy, in their attempt to improve the quality of life of the beneficiary population of free social interest housing, are limited by realities opposed to their goals, as in the case of Socio Vivienda 2 Program, in Guayaquil, and Huarcay, in Quito.
Space Theory
Authors: Chen S.
Published: May 2024

The author introduces a novel theoretical framework that suggests matter and energy are both converted from curved space. In Space Theory, gravitation is generated by the flow of space, instead of being transmitted by the graviton as in String Theory. This theory also suggests that Newton's gravitational constant, denoted as G, may not be truly constant but could vary over time. The equivalent equation of space is S=Ec2=mc4, and the gravitation of hollow sphere space is Sμν=4πGm=(4/3)π((r+a)3-r3). The Space Theory also predicts that the surface gravitational acceleration of the neutron star Crab Pulsar (PSR B0531+21) is approximately 8.21924883×106 m/s2.
Suppression PCR-Based Selective Enrichment Sequencing for Pathogen and Antimicrobial Resistance Detection on Cell-Free DNA in Sepsis - A Targeted, Blood Culture Independent Approach for Rapid Pathogen and Resistance Diagnostics in Septic Patients
Authors: Sonntag Mirko , Elgeti Vanessa K. , Vainshtein Yevhen , Jenner Lucca , Mueller Jan , Brenner Thorsten , Decker Sebastian O. , Sohn Kai
Published: May 2024

Sepsis is a life-threatening syndrome triggered by infection and accompanied by high mortality, with antimicrobial resistances (AMRs) further escalating clinical challenges. Rapid and reliable detection of causative pathogens and AMRs are key factors for fast and appropriate treatment, in order to improve outcome in septic patients. However, current sepsis diagnostics based on blood culture is limited by low sensitivity and specificity while current molecular approaches fail to enter clinical routine. Therefore, we developed a Suppression PCR-based selective enrichment sequencing approach (SUPSETS), providing a molecular method combining multiplex suppression PCR with Nanopore sequencing to identify most common sepsis-causative pathogens and AMRs using plasma cell-free DNA. Applying only 1 mL of plasma, we target eight pathogens across three kingdoms and ten AMRs in a proof-of-concept study. SUPSETS was successfully tested on first ten clinical samples and revealed comparable results to clinical metagenomics while clearly outperforming blood culture. Several clinically relevant AMRs could be detected additionally. Furthermore, SUPSETS provided first pathogen and AMR-specific sequencing reads within minutes of starting sequencing, thereby potentially decreasing time to results to 11 - 13 hours and suggesting diagnostic potential in sepsis.
Preharvest Elicitors as a Tool to Enhance Bioactive Compounds, Quality in Both Peel and Pulp of Yellow Pitahaya (Selenicereus megalanthus Haw) a Harvest and during Postharvest Storage
Authors: Erazo-Lara Alex Estuardo , García-Pastor María Emma , Padilla-González Pedro Antonio , Valero Daniel , Serrano María
Published: May 2024

Yellow pitahaya is a tropical fruit that has gained popularity in recent years. Natural elicitors are compounds that can stimulate the resistance and quality of fruits. The objective of this study was to evaluate the effects of natural elicitors: methyl salicylate (MeSa), methyl jasmonate (JaMe), salicylic acid (SA) and oxalic acid (OA) at concentrations of 0.1 mM (SaMe and JaMe) and 5 mM (SA and OA), applied to the yellow pitahaya fruits, under greenhouse conditions. After full blossom, 4 applications were made with a frequency of 15 days. At time of harvest and after storage, the following variables were evaluated: firmness (whole fruit), total soluble solids (TSS), total acidity (TA), phenolics and carotenoids (in the pulp), while phenolics, carotenoids, macronutrients and micronutrients were determined in the peel. The results showed that JaMe presented a delay of the ripening process, according to TSS, TA, firmness, and total phenolic compounds results, while MeSa advanced the fruit maturation. Regarding the alternative use of the peel as by-product, the application of natural elicitors significantly increased the content of polyphenols, carotenoids, macronutrients and micronutrients in the peel, which can be used as a bioactive compound in the food industry. In conclusion, the results indicate that natural elicitors can be an alternative to improve the quality and shelf life of yellow pitahaya fruits.
ChatGPT Impact on EFL Indian Undergraduates
Authors: Roy Kavita , Swargiary Khritish
Published: May 2024

This study investigates the impact of integrating ChatGPT on the academic writing performance and engagement of English as a Foreign Language (EFL) undergraduate students in Delhi, India, during the first semester of the academic year 2024/2025. It addresses the challenges within the Indian education system by exploring the effectiveness of AI-driven writing tools in enhancing writing skills and motivation among EFL learners. Employing a mixed-methods approach, the research evaluates ChatGPT's influence on 32 EFL Indian undergraduate students through draft revisions, questionnaires, and interviews. The findings highlight ChatGPT's positive impact on writing quality, engagement levels, and immediate feedback appreciation, emphasizing its potential in improving writing instruction and student outcomes in EFL contexts. Recommendations are provided for practitioners to integrate ChatGPT in writing exercises and for researchers to conduct longitudinal studies and explore ethical considerations in AI use for educational purposes, ultimately contributing to enhanced EFL education and technology-mediated learning.
CFO’s Intellectual Capital and its Relationship with Financial Performance, An Analysis of Companies Listed on the Stock Exchange (BOVESPA)
Authors: Jaqueline Valente , Moxotó Ana Claudia
Published: May 2024

In a business environment that is constantly changing, intellectual capital stands out as the most valuable asset for companies. Those who invest in developing their employees, especially their Chief Financial Officers (CFOs), tend to outperform their competitors. This study explores the relationship between the intellectual Capital of CFOs and the financial performance of companies listed on B3. The 100 companies with the largest capitalization listed on B3 in 2022 were analyzed, with available data on the CFO. A statistical model was applied in a cross-section analysis, considering two of the three subdivisions of Intellectual Capital: Human Capital ( experience, education, and certifications) and Relational/Social Capital ( network and reputation of the CFO). The results indicated a statistically significant relationship between CFOs' Human Capital level and the performance of the companies they manage, measured by EPS, ROA, and ROE. However, no statistical significance was found for Relational/Social Capital. This study contributes to the literature on intellectual capital and corporate finance in the Brazilian context, providing new evidence on the importance of CFOs' intellectual capital for company performance. It was observed that there are few equivalent studies in Brazil on the topic, especially about the Intellectual Capital of CFOs of companies listed on the stock exchange (BOVESPA).
Diagnostic Value of Menstrual Blood Lipidomics in Endometriosis: A Pilot Study
Authors: Starodubtseva Natalya , Chagovets Vitaliy , Tokareva Alisa , Dumanovskaya Madina , Kukaev Evgenii N. , Novoselova Anastasia , Frankevich Vladimir , Pavlovich Stanislav V. , Sukhikh Gennady Tikhonovich
Published: May 2024

Endometriosis is a prevalent chronic inflammatory disease characterized by a considerable delay between initial symptoms and diagnosis through surgery. The pressing need for a timely, non-invasive diagnostic solution underscores the focus of current research efforts. This study examines the diagnostic potential of the menstrual blood lipidome. The lipid profile of 39 samples (23 women with endometriosis and 16 patient of control group) was acquired using reverse-phase high-performance liquid chromatography-mass spectrometry with LipidMatch processing and identification. Profiles were normalized based on total ion counts. Significant differences in lipids were determined using the Mann-Whitney test. Lipids for the diagnostic model, based on logistic regression, were selected using a combination of variance importance projection filters and Akaike information criteria. Levels of ceramides, sphingomyelins, cardiolipins, triacylglycerols, acyl- and alkenyl-phosphatidylethanolamines, and alkenyl-phosphatidylcholines increased, while acyl- and alkyl-phosphatidylcholines decreased in cases of endometriosis. PE P-16:0/18:1 and CL 16:0_18:0_22:5_22:6 serve as marker lipids in the diagnostic model, exhibiting a sensitivity of 81% and specificity of 85%. The diagnostic approach based on dried spots of menstrual blood holds promise as an alternative to traditional non-invasive methods for endometriosis screening.
Environmental Filters Structure Cushion Bogs’ Floristic Composition along the Southern South American Latitudinal Gradient
Authors: Figueroa-Ponce Felipe , Hinojosa Luis Felipe
Published: May 2024

In southern South America, zonal flora, defined by macroclimatic conditions, is strongly influenced by an aridity filter near 29°S. Cushion bogs, high-altitude wetlands along the Andes, display homogeneous flora with cushion species dominance, contrasting with zonal vegetation. Despite being influenced by microclimatic conditions, these ecosystems experience varied environmental effects. This study identifies environmental filters affecting bog communities along a broad-scale latitudinal gradient from 15°S to 42°S.
We analyzed 420 bogs and 284 species across three macroclimatic regions with distinct summer, winter, and transitional arid rainfall regimes. Using variance partitioning and membership-based regionalization models, we examined the impacts of climatic, edaphic, and spatial variables on beta diversity. We also assessed species' niche overlap and the influence of environmental filters on the communities' phylogenetic diversity.
Results show that species turnover and niche overlap vary with macroclimatic differences, delineating three distinct regions. Notably, phylogenetic clustering in the driest part of the gradient (23°S —24°S) highlights the impact of the environmental filter. Aridity and temperature variations at a broad scale serve as environmental filters shaping the composition of bog communities across southern South America.
Effects of Sildenafil on the Recovery of Cognitive Function and the Protection of Neuronal Cell Death Following Transient Global Cerebral Ischemia in Gerbils
Authors: Yu Yeon Hee , Kim Gun Woo , Lee Yu Ran , Park Dae-Kyoon , Song Beomjong , Kim Duk-Soo
Published: May 2024

Cerebral ischemic stroke poses a significant global cause of death, with ischemia-reperfusion injury contributing to neuronal cell death and tissue damage. However, significant therapeutic approaches with meaningful treatment effects for patients with ischemic stroke in actual clinical practice are lacking. This study investigated the potential neuroprotective effects of sildenafil, a phosphodiesterase-5 inhibitor, in a global cerebral ischemia model. We explored the impact of sildenafil on GFAP and AQP-4 expression, which are markers associated with astrocyte activation and water homeostasis, respectively. The immunofluorescence analysis revealed that the number of cells co-expressing the markers, which increased in the ischemia-induced group, was significantly reduced in sildenafil-treated groups, suggesting a potential mitigating effect on ischemia-induced astrocyte activation. Additionally, we conducted diverse behavioral tests, including the open-field test, novel object recognition, Barnes maze, Y-maze, and passive avoidance tests, to assess the effect of sildenafil on the cognitive function impaired by ischemia. Cognitive improvements were observed following sildenafil administration (20mg/kg) compared to the untreated group. Taken together, the results presented here suggest sildenafil's potential as a neuroprotective agent for alleviating delayed neuronal cell death and cognitive function impaired by ischemia.
Rectangular Spiral Inward-Outward Alternating- Flow Polymer Thermal Collector for Solar Water Heating System—A Preliminary Investigation under Seri Iskandar, Malaysia Climate
Authors: Mohamad Taib Iskandar , Mohd Syaifudeen Mohammad Danish Shareeman , Nasir Abu Bakar
Published: May 2024

A flat-plate unglazed solar water heater (SWH) with polymer thermal absorber was developed and tested. Polymer thermal absorber as a viable alternative to metal thermal absorber that is found in most SWH systems. The performance of this polymer SWH system is measured based on inlet and outlet water temperature, water flowrate, ambient air temperature and solar irradiance. The polymer thermal absorbers are hollow Polyvinyl Chloride (PVC) pipes with 20mm external diameter and 3 mm thick, painted in black to enhance radiation absorption. The pipes are arranged in a rectangular spiral inward-outward opposing-flow (RSioof). The collector pipes are placed in a 1 m2 square enclosure with bottom insulation and reflective surface for better radiation capture. Water circulation is a closed loop with a 16-liter uninsulated storage tank, driven by a pump and controlled by valves to maintain a mass flow rate of 0.00031 to 0.00034 kg/s. The test was conducted under partially clouded sky from 9 am to 5 pm, with solar irradiance between 105 and 1003 W/m2 and 27-36oC ambient air temperature. The SHW produced outlet hot water at 65oC by mid-day and maintained the storage temperature at 63oC until the end of test duration. Photo-thermal energy conversion was recorded with 23% maximum value. Results indicate that a flat plate solar water heater with polymer thermal absorber in RSioof design can be an effective alternative to the metal thermal absorber solar water heater and can be further optimized with glazing and tube sizing.
Getting Grip on Phosphorus: Potential of Microalgae as a Vehicle for Sustainable Usage of This Macronutrient
Authors: Solovchenko Alexei E. , Plouviez Maxence , Khozin-Goldberg Inna
Published: May 2024

Phosphorus (P) is an important and irreplaceable macronutrient. It is central to energy and information storage and exchange in living cell. P is an element with “broken geochemical cycle” since it lacks abundant volatile compounds capable of closing the P cycle. P fertilizers are critical for global food security, but the reserves of minable P are scarce and non-evenly distributed between countries of the world. Accordingly, the risks of global crisis due to limited access to P reserves is expected to be graver than those entailed by competition for fossil hydrocarbons. Paradoxically, despite the scarcity and value of P reserves, its usage is extremely inefficient: current waste rate reaches 80% giving rise to a plethora of unwanted consequences such as eutrophication leading to harmful algal blooms. Microalgal biotechnology comprise a promising solution to tackle this challenge. The proposed review briefly presents the relevant aspects of microalgal P metabolism such as cell P reserve composition and turnover, and the regulation of P uptake kinetics for maximization of P uptake efficiency with the focus on novel knowledge. Multifaceted role of polyPhosphates, the largest cell depot for P, is discussed with emphasis on the P toxicity mediated by short-chain polyPhosphates. Opportunities and hurdles of P bioremoval via P uptake from waste streams with microalgal cultures, either suspended or immobilized, is discussed along with the role of bacterial components of microalgal-bacterial consortia in these processes. Possible avenues of P-rich microalgal biomass such as biofertilizer production or extraction of valuable polyPhosphates and other bioproducts are considered. The review concludes with a comprehensive assessment of the current potential of microalgal biotechnology for ensuring the sustainable usage of phosphorus.
A Comparative Analysis of Librarians’ Views on AI Support for Learning Experiences, Lifelong Learning, and Digital Literacy in Malaysia and Indonesia
Authors: Mutia Fitri , Masrek Mohamad Noorman , Baharuddin Mohammad Fazli , Shuhidan Shamila Mohamed , Soesantari Tri , Yuwinanto Helmy Prasetyo , Atmi Ragil Tri
Published: May 2024

This research delves into librarians' perceptions of artificial intelligence (AI) support in learning experiences, lifelong learning, and digital literacy within the library context, undertaking a comparative analysis between Malaysia and Indonesia. Employing a survey methodology, a self-developed questionnaire was administered to 59 librarians in Malaysia and 85 in Indonesia. The questionnaire, utilizing a Likert scale, systematically explored subjective judgments on AI's impact across various dimensions. A noteworthy finding is the consistent and harmonized perception among librarians in both countries, underscoring a universal recognition of AI's perceived support for learning experiences, lifelong learning, and digital literacy. Of particular interest is the emphasis on sustainability in librarians' perspectives, as they recognize the enduring value of AI in fostering educational growth. Despite the limitations inherent in perceptual measures and a relatively modest sample size, the empirical and practical contributions of this study are significant. It offers a nuanced exploration of librarians' viewpoints, providing valuable insights for future research endeavors. Moreover, the study's findings have immediate practical implications for the integration of AI tools in library services, policy-making, and professional development, with a focus on ensuring sustainable implementation for long-term benefits.
Acid Adaptation Enhances Tolerance of Escherichia coli O157:H7 to High Voltage Atmospheric Cold Plasma in Raw Pineapple Juice
Authors: Little Allison , Mendonca Aubrey Francis , Dickson James , Fortes-Da-Silva Paulo , Boylston Terri , Lewis Braden , Coleman Shannon , Thomas-Popo Emalie
Published: May 2024

Pathogens that adapt to environmental stress can develop an increased tolerance to some physical or chemical antimicrobial treatments. The main objective of this study was to determine if acid adaptation increased the tolerance of Escherichia coli O157:H7 to high voltage atmospheric cold plasma (HVACP) in raw pineapple juice. Samples (10 ml) of juice were inoculated with non-acid-adapted (NAA) or acid adapted (AA) E. coli to obtain a viable count of ~ 7.00 log10 CFU/ml. The samples were exposed to HVACP (70 kV) for 1 to 7 min with inoculated non-HVACP-treated juice serving as control. Juice samples were analyzed for survivors at 0.1 h, and after 24 h of refrigeration (4 °C). Samples analyzed after 24 h exhibited significant decreases in viable NAA cells with sub-lethal injury detected in both NAA and AA survivors (P<0.05). No NAA survivor in juice exposed to HVACP for 5 or 7 min was detected after 24h. However, numbers of AA survivors were 3.33 and 3.09 log10 CFU/ml in juice treated for 5 and 7 min, respectively (P<0.05). These results indicate that acid adaptation increases the tolerance of E. coli to HVACP in pineapple juice. The potentially higher tolerance of AA E. coli O157:H7 to HVACP should be considered in developing safe juice processing parameters for this novel non-thermal technology.
Impact of Smoking Cessation and Charlson Comorbidity Index on Influenza Vaccination Efficacy in COPD Patients
Authors: Chang Hui-Chuan , Liu Shih-Feng
Published: May 2024

Chronic obstructive pulmonary disease (COPD) patients are particularly susceptible to respiratory infections like influenza, which exacerbate symptoms and increase healthcare utilization. While smoking cessation and influenza vaccination are recommended preventive measures, their combined impact on healthcare resource utilization is underexplored. The Charlson Comorbidity Index (CCI) assesses comorbidity burden in COPD patients and may influence healthcare outcomes. We conducted a retrospective analysis of 357 COPD patients, evaluating smoking cessation success over one year and influenza vaccination receipt, stratifying patients by CCI scores. Healthcare utilization outcomes included emergency room visits, hospitalizations, and medical expenses. Results showed that 51.82% of patients quit smoking and 59.66% received influenza vaccination, with higher comorbidity prevalence in advanced COPD stages (p=0.002). Both smoking cessation and influenza vaccination independently correlated with decreased emergency room visits, hospital admissions, days, and costs. Patients who both quit smoking and received influenza vaccination exhibited the lowest healthcare utilization rates. In conclusion, smoking cessation and influenza vaccination significantly reduce healthcare resource utilization in COPD patients, with the combination yielding synergistic benefits, particularly in those with lower CCI scores. Integrating these interventions and comorbidity management in COPD strategies is essential for optimizing patient outcomes and healthcare efficiency.
An $E_8 \otimes E_8$ Unification of the Standard Model with Pre-Gravitation, on an Exceptional Lie algebra - Valued Space
Authors: KAUSHIK PRIYANK , VAIBHAV VATSALYA , SINGH TEJINDER PAL
Published: May 2024

We propose an $E_8 \otimes E_8$ unification of the standard model with pre-gravitation, on an exceptional Lie algebra-valued space . Each of the $E_8$ has in its branching an $SU(3)$ for space-time and an $SU(3)$ for three fermion generations. The first $E_8$ further branches to the standard model $SU(3)_c \otimes SU(2)_L \otimes U(1)_Y$ and describes the gauge bosons, Higgs and the left chiral fermions of the standard model. The second $E_8$ further branches into a right-handed counterpart (pre-gravitation) $SU(3)_{grav}\otimes SU(2)_R \otimes U(1)_g$ of the standard model, and describes right chiral fermions, a Higgs, and twelve gauge bosons associated with pre-gravitation, from which general relativity is emergent. We account for 208 out of the 496 degrees of freedom of $E_8 \otimes E_8$ and propose an interpretation for the remaining 288, motivated by the trace dynamics Lagrangian of our theory. We explain how the two copies of $SU(3)_{spacetime}$ together give rise to a 6D spacetime with signature $(3,3)$ which upon symmetry breaking gives rise to our 4D spacetime and to a second 4D anti-spacetime with flipped signature.
Impaired Tertiary Dentin Secretion After Shallow Injury in Tgfbr2-Deficient Dental Pulp Cells Is Rescued by Extended CGRP Signaling
Authors: Stanwick Monica , Fenesha Fatma , Hamid Ahmed , Kang Khushroop , Kanniard Dane , Kim Irene , Mandarano Nicholas , Schumacher Fernanda L , Peters Sarah B.
Published: May 2024

Introduction: Transforming growth factor beta receptor 2 (Tgfbr2) signaling regulates odontoblast differentiation, dentin mineralization, and the paracrine signaling that guides sensory innervation in developing mouse molars. We hypothesized that Tgfbr2 also regulates the neuro-pulpal responses to injury to activate axon sprouting and tertiary dentin secretion. Methods: We per-formed a timed deletion of Tgfbr2 using tetracycline-responsive Osterix-Cre;Tgfbr2f/f (Tgfbr2^cko) mice, which allowed them to develop normally. At 3 months, we drilled a shallow hole on the mesial side of the first mandibular molar (M1) in both control and Tgfbr2^cko mice. Hemi-mandibles were collected 4, 8, 21, and 56 days post-injury (dpi). In situ hybridization (ISH) for Sp7 was performed to confirm Osterix-Cre transcription. Microcomputed tomography (micro-CT) imaging and histology were used to examine the tertiary dentin, and immunofluorescence was used to visualize CGRP+ axons following the injury. Results: Tgfbr2 deletion was inferred by Sp7 ISH. Micro-CT and histology indicated a lower dentin volume in 21 dpi Tgfbr2^cko M1s compared to WT M1s, but the volume was comparable by 56 dpi. The duration of axon sprouting was longer in injured Tgfbr2^cko M1s compared to WT M1s. Conclusions: These results suggest that sensory afferents may support dentin repair in Tgfbr2-deficient odontoblasts.
Entire-range Cooperative Trajectory Planning of Gliding-guided Projectiles with Weak Maneuverability
Authors: Yin Qiulin , Chen Qi , Wang Zhongyuan , Wang Qinghai
Published: May 2024

In modern warfare, the diversified tasks can sometimes be challenging to fulfill with a single projectile, necessitating the incorporation of cooperation constraints in trajectory planning. Limited by the stringent requirements of the launch platform and lower costs, the maneuverability and ability to adjust the flight time range of projectiles are weak. Therefore, the rationality of performance indicators and flight time coordination strategies is especially critical. To fully exploit the control capabilities of projectiles, a battlefield area segmentation method is proposed, differentiated performance indicators and a cooperative trajectory planning model that considers the entire range of projectiles are established. Considering the limitations of existing flight time coordination strategies in applications, targeted improvements have been made in different cooperation scenarios, and a bi-level adaptive cooperation strategy(BACS) with both optimality and flexibility is proposed. To rapidly determine feasible conditions, a simple and universal method for obtaining the capability ranges of cooperation is introduced. The multi-phase optimal control problems are transformed into nonlinear programming problems using the Radau pseudo-spectral method, and simulation results show that the proposed methods are capable of handling trajectory planning tasks in different cooperation scenarios. The improved strategies effectively enhance the planning success rate under relatively harsh conditions and BACS demonstrates good compatibility with the problems studied.
Into Mitigating Making-Do Practices using the Last Planner System and BIM: A System Dynamic Analysis
Authors: Karaz Mahmoud , Teixeira José Manuel Cardoso , Amaral Tatiana Gondim do
Published: May 2024

Effective waste elimination is critical for the success of construction projects. Although several studies have focused on various aspects of construction waste, limited efforts have investigated the dynamic effect of Making-Do (MD) practices on productivity, rework, defects, and materials wastage. From a lean construction perspective, this study aims to address MD waste using the Last Planner System (LPS) and BIM. First, the casual structure that can cause MD in construction projects was expressed in a casual loop diagram (CLD), and thematic analysis uncovered the strategies of LPS-BIM to eliminate MD identified by reviewing the literature. Secondly, twenty-five strategies from the LPS and BIM strategies to address MD using Structural Equation Modeling (SEM) were assessed. Subsequently, a system dynamics model (SDM) for investigating LPS-BIM strategies on MD decisions in a construction project was formulated based on the underlying causal loop diagrams and the mathematical relations among the variables. Finally, the model was applied to three projects, and simulations for four LPS-BIM scenarios were carried out. The findings show that dynamic interactions among diverse production planning and control factors are critical in evaluating MD impacts on a construction project. The results demonstrate that the LPS-BIM approach resulted in an average 43.8% reduction in the tasks performed with MD, 45.3% of constraints, 66.5% of construction waste, increasing 13.7% completion rate, and 29.3% cost reduction, demonstrating that LPS-BIM as a more efficient solution for MD mitigation and construction planning.
Supporting Multi-Stakeholder Participation Processes: A Serious Game Application for Watershed Management in Colombia
Authors: Gonzalez Camilo , Moncada Angelica , Santos Tania Fernanda Santos , Rincón Wilford , Coleoni Claudia , Macura Biljana
Published: May 2024

Multi-stakeholder participation processes in watershed management face challenges due to limited monitoring and baseline data, resulting in a lack of awareness among stakeholders about the current state of the watershed. This knowledge gap often leads to conflicts of interest, where the broader impacts of individual decisions are overlooked. To overcome these limitations, this paper explores the design and implementation of a Serious Game (SG) aimed at co-producing a watershed management plan at the basin scale within the specific context of the Campoalegre River basin in Colombia. By providing an interactive platform, the SG facilitates collaboration between local actors, who may be unfamiliar with existing watershed plans, and decision-makers. The goal is to create a participatory space where stakeholders can comprehend the watershed management plan structure and prioritize actions based on various climatic, social, and economic conditions. Following the application of the SGs, stakeholders demonstrated an improved understanding of the basin, fostering increased participation, open debate, and the proposal of actions. These outcomes serve as valuable inputs for the implementation of water management planning policies, showcasing the potential of SGs in bridging knowledge gaps, and fostering effective multi-stakeholder engagement.
A Survey in Eastern Europe on the Evaluation of Umami Taste
Authors: Iannilli Emilia , Schienle Anne
Published: May 2024

The umami taste, one of the five primary tastes, has been shown to be a helpful tool in reducing salt intake and improving eating patterns. This study aimed to investigate how umami taste is perceived and evaluated in a sample of adult participants living in Eastern Europe, taking into account their sex, education, and travel habits. A Text Semantic-mining Analysis (TSA) was conducted using data from 300 adults (152 males and 148 females, mean age = 26.71 years). They also performed an MSG and NaCl (salt) discrimination test and were further interviewed about taste preferences, travel habits, and type of residence. The results indicated that the taste sensation elicited by the MSG solution was complex, unfamiliar and had negative valence. The descriptor most commonly used for MSG was "sour," followed by "bitter" and "salty" for the whole group, but these differed by gender. There was no difference in umami preference between males and females, but males preferred spicy flavors while females favored sweet and salty tastes. This finding underscores the potential bias and unknown significance of umami taste, suggesting the importance of establishing an accurate concept in European culture and promoting its usage in food choices for better health.
Simulated And Experimental Study Of The Available Energy Transfer From An Ev Battery In A V2g System
Authors: Armenta-Deu Carlos , de la Malla Fernando
Published: May 2024

ABSTRACT
The work is focused on determining the energy transfer from EV’s to grid for different operating conditions. The study has been simulated on a scaled model and later validated through experimental tests on batteries of 20 kWh, 40 kWh, 60 kWh and 80 kWh for EV’s energy rates from 130 Wh/km to 180 Wh/km. Tests have been developed to evaluate the energy transfer from driving distance between 0 and 50 km. Results have proved there is a linear dependence between energy transfer and energy rate. Experimental and simulated values have been correlated showing a 99.6% accuracy. The ratio of energy transfer to battery energy capacity depends not only on this latter parameter, but also on the energy rate of the electric vehicle. Experimental tests have shown a variation from a minimum transfer ratio of 54.1% for the highest electric vehicle energy rate and lowest battery energy capacity and power transfer to grid rate to a maximum of 88.6% for the highest power transfer to grid rate and battery energy capacity and lowest electric vehicle energy rate.
Early 2-Factor Transcription Factors Associated with Progression and Recurrence in Bevacizumab-Responsive Subtypes of Glioblastoma
Authors: Shi Jian
Published: May 2024

The early 2-factor (E2F) family of transcription factors, including E2F1-8, plays a critical role in apoptosis, metabolism, proliferation, and angiogenesis within glioblastoma (GBM). However, the specific functions of E2F transcription factors (E2Fs) and their impact on the malignancy of Bevacizumab (BVZ)-responsive GBM subtypes remain unclear. This study used data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) to explore the impact of eight E2F family members on the clinical characteristics of BVZ-responsive GBM subtypes and possible mechanisms of recurrence after BVZ treatment.To predict the possibility of GBM patient survival and progression, we classified and comparedthe expression of E2Fs according to BVZ-responsive subtypes and employed a machine learning method-TreeBagger, one random forest algorithm. Multiple bioinformatics analyses have shown that the significant increased E2F8 post BVZ treatment may enhance the function of angiogenesis and stem cell proliferation, indicating one of the mechanisms of GBM recurrence after treatment. In addition, BVZ treatment in unresponsive GBM patients may potentially worsen disease progression. Our findings suggest that E2F family members play important roles in GBM malignancy and BVZ treatment response, highlighting their potential as prognostic biomarkers and therapeutic targets and recommending precise BVZ treatment for GBM patients.
Epirubicin and Non-Muscle Invasive Bladder Cancer Treatment: A Systematic Review
Authors: Chiujdea Sever , Ferro Matteo , Vartolomei Mihai Dorin , Lucarelli Giuseppe , Bekku ,Kensuke , Matsukawa Akihiro , Parizi Mehdi Kardoust , Klemm Jakob , Tsuboi Ichiro , Fazekas Tamas , Mancon Stefano , Shariat Shahrokh F.
Published: May 2024

(1) Background: Intravesical Chemotherapy is standard of care in intermediate risk non-muscle-invasive bladder cancer (NMIBC). Different agents are used across the world based on availability, cost and practice patterns. Epirubicin (EPI), one of these agents is used by many centers over many decades. However, its true differential efficacy compared to other agents and its tolerability is still poorly reported. We aimed to assess the differential efficacy and safety of intravesical EPI in NMIBC patients. (2) Methods: A systematic search of PUBMED, Webofscience, clinicaltrials.gov, and Google Scholar databases was performed on December 31, 2023. We reviewed 28 studies that included patients treated EPI after TURBT (transurethral resection of bladder tumors) for NMIBC and who compared oncological outcomes such as recurrence and progression to other adjuvant treatment such as Mitomycin C (MMC), Gemcitabine (GEM) and BCG. Further, we looked at the safety profile of EPI at room temperature under hyperthermia intravesically and at the oncological outcomes when administrated with hyperthermia; (3) Results: When compared to other chemotherapeutics (MMC/GEM), EPI seemed to be equally effective; however, EPI seems less effective in preventing disease management recurrence compared to BCG intravesical. The most common adverse events of EPI include cystitis, dysuria, and pollakiuria. (4) Conclusions: EPI exhibits similar oncological performances to Gemcitabine and Mitomycin C currently used for adjuvant therapy in NMIBC. Novel delivery mechanisms such as hyperthermia are promising newcomers.