bluuebunny/crossref_metadata_2025 huggingface dataset:
An agricultural community’s perspectives on COVID-19 testing to support safe school reopening
Authors: Ramirez Magaly , Bishop Sonia , Ibarra Genoveva , Shah Parth , Duran Miriana C. , Chae Hwa Young , Hassell Laurie , Garza Lorenzo , Linde Sandra , Garrison Michelle M. , Drain Paul K. , Ko Linda K.
Published: August 2023

Introduction

School-based COVID-19 testing may be an effective strategy for reducing transmission in schools and keeping schools open. The study objective was to examine community perspectives on school-based COVID-19 testing as a mitigation strategy to support safe school reopening.

Methods

We conducted a qualitative study in Yakima County, an agricultural region of Washington state, where over half of residents are Hispanic/Latino. From June to July 2021, we interviewed 18 students (13 years old, on average) and 19 school employees, and conducted four focus groups (2 in Spanish, 2 in English) with 26 parents. We audio-recorded the semi-structured interviews and focus group discussions which were then transcribed. We used an inductive, constant comparison approach to code the transcripts and conducted a thematic analysis to generate themes.

Results

We identified four main themes. Students, parents, and school employees desired a return to in-person learning (Theme 1). Schools implemented numerous COVID-19 mitigation strategies (e.g., masking) to facilitate a safe return to school but felt that adding testing would not be feasible due to a lack of resources and overworked staff (Theme 2). Parents and school employees’ familiarity with COVID-19 testing procedures influenced their support for testing (Theme 3). Parents and school employees felt there were inadequate resources for individuals who test positive for COVID-19 (Theme 4).

Discussion

Schools require adequate resources and medical personnel to implement COVID-19 testing. Individuals also need resources after testing positive, including physical space to isolate, financial resources for those without paid time off, and delivery of food and other necessities to households in rural communities.
Perception of consanguineous marriage among the qatari population
Authors: Abdu Yasamin , Ahmed Khalid , Ibrahim Mohamed Izham Mohamed , Abdou Mariam , Ali Arwa , Alsiddig Hind , Selim Nagah A. , Yassin Mohammed A.
Published: August 2023

Background

Hereditary blood diseases are widespread among the Arab population due to the high rates of consanguineous marriages; research regarding the perception of consanguineous marriage in some countries, such as Qatar, is extremely scarce. Therefore, this study aimed to investigate the prevalence of consanguineous marriage and assess the perception of consanguineous marriage among the Qatari population.

Methods

A cross-sectional study used a self-administered questionnaire among 395 Qatari adults aged 18–35 who attended primary healthcare institutions in Qatar. A convenience sampling technique was used to select the study participants. An independent t-test was used to compare the significance of the mean between the two groups with positive and negative perceptions of consanguineous marriage. Categorical data were analyzed for association using the chi-square or Fisher's exact test. Finally, a multiple logistic regression analysis was conducted to determine the significant predictors of the positive perception of consanguineous marriage. A significant level was set at p < 0.05.

Results

Approximately 45% of the participants had a positive perception toward consanguineous marriage, and the most common reason stated by those participants was “habit and traditions.” The prevalence of consanguineous marriage among married couples was 62.6%, and among those with consanguineous marriage, most were married to first cousins (81.7%). Moreover, compared to the participants with negative perceptions of consanguineous marriage, those with positive ones were significantly older, married, with lower educational levels and higher monthly income levels, did not hear about glucose-6-phosphate dehydrogenase (G6PD) deficiency, did not know what kinds of diseases are being screened in the premarital test, and were married to a relative.

Conclusion

The prevalence of consanguineous marriage is high among the Qatari population, and this requires an immediate need for community-based campaigns to raise public awareness about the problem and its potential impact.
Dynamic redistribution and inheritance of chromatin:RNA interactions during cell division
Authors: Sparago Emily , Watanabe Reito , Sharp Judith A. , Blower Michael D.
Published: August 2023

During interphase, the nucleus contains a multitude of transcripts that influence the function of chromatin and global structure of the nucleus. Nuclear transcripts include nascent mRNAs in the process of transcription and mRNA processing, spliceosomal RNAs which catalyze mRNA processing, rRNAs that are being transcribed and processed to assemble functional ribosomes, and sno- and scaRNAs that participate in rRNA processing and modification. In addition, there are long noncoding RNAs (lncRNA) that associate with chromatin to control gene expression, or can even influence locus function in the case of centromeres and telomeres. Most of our knowledge of the functions of nuclear RNAs come from studies of interphase cells when the nuclear envelope separates nuclear and cytoplasmic contents. However, during mitosis the nuclear envelope breaks down, resulting in the mixing of nuclear and cytoplasmic components. Much less is known about the regulation and function of nuclear RNAs during mitosis. In this review, we discuss the cell cycle-dependent localization of different categories of RNAs, how the trans-acting factors SAF-A and Ki-67 regulate mitotic RNA localization, and describe how select categories of RNAs are inherited from the previous cell cycle in G1.
Effect of classical music on growth performance, stress level, antioxidant index, immune function and meat quality in broilers at different stocking densities
Authors: Gao Xinlei , Gong Jiangang , Yang Bowen , Liu Yanci , Xu Hongjian , Hao Yanshuang , Jing Jialin , Feng Zhihua , Li Lihua
Published: August 2023

High-stocking density is one of the factors that can easily cause oxidative stress and inflammatory reaction of broilers. Currently, music therapy has been proposed to help animals relieve stress to some extent. However, it is still unclear whether classical music can alleviate stress in broilers at high stocking densities. Hence, this study aimed to investigate the effects of classical music on growth performance, stress level, antioxidant index, immune function and meat quality of broilers under different stocking densities. A total of 540 one-day-old broilers with similar body weight were randomly divided into 6 treatment groups, with 6 replicates per group, which included two feeding environments (with/without classical music) and three stocking densities (15.5, 17.9, and 20.3 birds/m2), thereby making a 2 × 3 factorial arrangement. The results showed as follows: increasing stocking density decreased the average daily feed intake and average daily gain (ADG), increased feed-to-gain ratio (F/G) and mortality of broilers. Moreover, increased density resulted in an increase in serum corticosterone (CORT) and adrenocorticotropic hormone (ACTH) levels. Increasing stocking density decreased spleen and bursal indices, serum immunoglobulin A (IgA), immunoglobulin G (IgG), and immunoglobulin M (IgM) levels. Increasing stocking density elevated serum malondialdehyde (MDA) and decreased catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) activities. Increasing stocking density decreased serum total protein (TP) levels and increased total cholesterol (TC) and glucose (GLU) levels. Additionally, increasing stocking density decreased the cooking liss of pectoralis and increased the L*24h value of pectoralis. Meanwhile, playing classical music for broilers increased their ADG and decreased F/G, and decreased serum CORT, ACTH, GLU content. In addition, the bursa of Fabricius index, serum IgA and IgG contents as well as the a*24h value of pectoralis was increased under the music therapy. In conclusion, high-stocking density (20.3 birds/m2) harmed the growth performance and health of broilers, and the classical music stimulus ameliorated the negative effects to some extent.
Applying Particle Swarm Optimization Variations to Solve the Transportation Problem Effectively
Authors: Aroniadi Chrysanthi , Beligiannis Grigorios N.
Published: August 2023

The Transportation Problem (TP) is a special type of linear programming problem, where the objective is to minimize the cost of distributing a product from a number of sources to a number of destinations. Many methods for solving the TP have been studied over time. However, exact methods do not always succeed in finding the optimal solution or a solution that effectively approximates the optimal one. This paper introduces two new variations of the well-established Particle Swarm Optimization (PSO) algorithm named the Trigonometric Acceleration Coefficients-PSO (TrigAc-PSO) and the Four Sectors Varying Acceleration Coefficients PSO (FSVAC-PSO) and applies them to solve the TP. The performances of the proposed variations are examined and validated by carrying out extensive experimental tests. In order to demonstrate the efficiency of the proposed PSO variations, thirty two problems with different sizes have been solved to evaluate and demonstrate their performance. Moreover, the proposed PSO variations were compared with exact methods such as Vogel’s Approximation Method (VAM), the Total Differences Method 1 (TDM1), the Total Opportunity Cost Matrix-Minimal Total (TOCM-MT), the Juman and Hoque Method (JHM) and the Bilqis Chastine Erma method (BCE). Last but not least, the proposed variations were also compared with other PSO variations that are well known for their completeness and efficiency, such as Decreasing Weight Particle Swarm Optimization (DWPSO) and Time Varying Acceleration Coefficients (TVAC). Experimental results show that the proposed variations achieve very satisfactory results in terms of their efficiency and effectiveness compared to existing either exact or heuristic methods.
Data-Driven Deployment of Cargo Drones: A U.S. Case Study Identifying Key Markets and Routes
Authors: Bridgelall Raj
Published: August 2023

Electric and autonomous aircraft (EAA) are set to disrupt current cargo-shipping models. To maximize the benefits of this technology, investors and logistics managers need information on target commodities, service location establishment, and the distribution of origin–destination pairs within EAA’s range limitations. This research introduces a three-phase data-mining and geographic information system (GIS) algorithm to support data-driven decision-making under uncertainty. Analysts can modify and expand this workflow to scrutinize origin–destination commodity flow datasets representing various locations. The algorithm identifies four commodity categories contributing to more than one-third of the value transported by aircraft across the contiguous United States, yet only 5% of the weight. The workflow highlights 8 out of 129 regional locations that moved more than 20% of the weight of those four commodity categories. A distance band of 400 miles among these eight locations accounts for more than 80% of the transported weight. This study addresses a literature gap, identifying opportunities for supply chain redesign using EAA. The presented methodology can guide planners and investors in identifying prime target markets for emerging EAA technologies using regional datasets.
Sustainable Greenhouse Covering Materials with Nano- and Micro-Particle Additives for Enhanced Radiometric and Thermal Properties and Performance
Authors: Maraveas Chrysanthos , Kotzabasaki Marianna I. , Bayer Ilker S. , Bartzanas Thomas
Published: August 2023

This review aims to provide a comprehensive overview of nano- and microscopic materials that can provide thermal radiation insulation without reducing visible light transmittance, thereby reducing heat loss and conserving energy in greenhouses. We also reviewed the radial and thermal properties of greenhouse covering materials. Fillers, colorants, reinforcers, and additives, as well as glass, plastic film, and plastic sheet materials, were discussed. Additionally, by searching for keywords like insulation film, insulation agent, and infrared insulation, compounds based on graphene and fullerene as well as phase transition materials (PCMs) that may be used for radiation insulation, we proposed their potential use in greenhouse covers. They can be divided into semi-transparent photovoltaic (PV) materials, zinc oxide-based film fillers, and silica filter films. We discussed the radiation heat insulation and light transmission characteristics of these materials. Nano-synthesis techniques were also investigated. Based on latest advances in the literature, future developments in the micro- and macroscale synthesis of nanomaterials will enable additional innovations in covering materials for greenhouse structures. A limiting factor, though, was the high sensitivity of PVs to external climatic and meteorological variables. The ability of materials used to make greenhouse covers to control the microclimate, reduce CO2 emissions, use less energy, and increase agricultural productivity, however, cannot be disputed. Similar to this, a thorough examination of the uses of various greenhouse technologies reveals that the advancements also have financial advantages, particularly in terms of reducing greenhouse heating and cooling expenses. The PCMs, which decreased greenhouse-operating costs by maintaining constant ambient temperatures, provide ample evidence of this.
A Quantitative Histologic Analysis of Oogenesis in the Flatfish Species Pleuronectes platessa as a Tool for Fisheries Management
Authors: Sauger Carine , Quinquis Jérôme , Berthelin Clothilde , Lepoittevin Mélanie , Elie Nicolas , Dubroca Laurent , Kellner Kristell
Published: August 2023

The following paper gives a detailed description of the oogenesis cycle for the European Plaice (Pleuronectes platessa), from oogonia to post-ovulatory follicle, including ovarian follicle and zona pellucida sizes. Noteworthy particularities were the difficulty in identifying cortical alveoli due to their very small size. Quantitative histology (stereology) on histological slides was used to determine a first size at maturity for females from the English Channel, which was found to be smaller compared to the literature (19 cm). Stereology also determined a first spawning event starting in January, with a peak in February and ongoing until March. Moreover, the use of stereology showed misclassifications for individuals categorized into a maturity phase using a macroscopic visual method. Misclassifications were found with individuals that had spawned (D) but were put under the immature (A) phase, and individuals in development (B) classified under D.
Effect of a High-Starch or a High-Fat Diet on the Milk Performance, Apparent Nutrient Digestibility, Hindgut Fermentation Parameters and Microbiota of Lactating Cows
Authors: Liu Suran , Wei Ziwei , Deng Ming , Xian Zhenyu , Liu Dewu , Liu Guangbin , Li Yaokun , Sun Baoli , Guo Yongqing
Published: August 2023

In this study, changes in milk performance, nutrient digestibility, hindgut fermentation parameters and microflora were observed by inducing milk fat depression (MFD) in dairy cows fed with a high-starch or a high-fat diet. Eight Holstein cows were paired in a completely randomized cross-over design within two 35 d periods (18 d control period and 17d induction period). During the control period, all cows were fed the low-starch and low-fat diet (CON), and at the induction period, four of the cows were fed a high-starch diet with crushed wheat (IS), and the other cows were fed a high-fat diet with sunflower fat (IO). The results showed that, compared to when the cows were fed the CON diet, when cows were fed the IS or IO diet, they had lower milk fat concentrations, energy corrected milk, 3.5% fat-corrected milk yield, feed efficiency and apparent digestibility of NDF and ADF. However, cows fed the IO diet had a lower apparent digestibility of ether extracts. In addition, we observed that when cows were fed the high-starch (IS) or high-fat (IO) diet, they had a higher fecal concentration of propionate and acetate, and a lower NH3-N. Compared to when the cows were fed the CON diet, cows fed the IS diet had a lower pH, and cows fed the IO diet had a lower concentration of valerate in feces. In the hindgut microbiota, the relative abundance of Oscillospiraceae_UCG-005 was increased, while the Verrucomicrobiota and Lachnospiraceae_AC2044_group were decreased when cows were fed the IO diet. The relative abundance of Prevotellaceae_UCG-003 was increased, while the Alistipes and Verrucomicrobiota decreased, and the Treponema, Spirochaetota and Lachnospiraceae_AC2044_group showed a decreasing trend when cows were fed the IS diet. In summary, this study suggested that high-starch or high-fat feeding could induce MFD in dairy cows, and the high-fat diet had the greatest effect on milk fat; the high-starch or high-fat diet affected hindgut fermentation and apparent fiber digestibility. The changes in hindgut flora suggested that hindgut microbiota may be associated with MFD in cows.
Short-Term Storage of Rooster Ejaculates: Sperm Quality and Bacterial Profile Differences in Selected Commercial Extenders
Authors: Tvrdá Eva , Petrovičová Michaela , Ďuračka Michal , Benko Filip , Slanina Tomáš , Galovičová Lucia , Kačániová Miroslava
Published: August 2023

Bacterial contamination of semen has become an important contributor to the reduced shelf life of insemination doses in the poultry industry, which is why antibiotics (ATBs) are an important component of semen extenders. Due to a global rise in antimicrobial resistance, the aim of this study was to assess the efficiency of selected commercially available semen extenders to prevent possible bacterial contamination of rooster ejaculates. Two selected extenders free from or containing 31.2 µg/mL kanamycin (KAN) were used to process semen samples from 63 healthy Lohmann Brown roosters. Phosphate-buffered saline without ATBs was used as a control. The extended samples were stored at 4 °C for 24 h. Sperm motility, viability, mitochondrial activity, DNA integrity and the oxidative profile of each extended sample were assessed following 2 h and 24 h of storage. Furthermore, selective media were used to quantify the bacterial load and specific bacterial species were identified with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The results indicate that semen extenders enriched with KAN ensured a significantly higher preservation of sperm quality in comparison to their KAN-free counterparts. Bacterial load was significantly decreased in diluents supplemented with ATBs (p ≤ 0.001); however, KAN alone was not effective enough to eradicate all bacteria since several Escherichia coli, Enterococcus faecalis, Enterococcus faecium and Micrococcus luteus were retrieved from samples extended in KAN-supplemented commercial extenders. As such, we may suggest that more focus should be devoted to the selection of an optimal combination and dose of antibiotics for poultry extenders, which should be accompanied by a more frequent bacteriological screening of native as well as extended poultry semen.
Reviewing the Current Understanding of Replant Syndrome in Orchards from a Soil Microbiome Perspective
Authors: Newberger Derek R. , Manter Daniel K. , Vivanco Jorge M.
Published: August 2023

Replant syndrome (RS) of fruit and nut trees causes reduced tree vigor and crop productivity in orchard systems due to repeated plantings of closely related tree species. Although RS etiology has not been clearly defined, the causal agents are thought to be a complex of soil microorganisms combined with abiotic factors and susceptible tree genetics. Different soil disinfection techniques alleviate RS symptoms by reducing the loads of the deleterious microbiome; however, the positive effect on crop growth is temporary. The goals of this paper are: (1) to conceptualize the establishment of the syndrome from a microbiome perspective and (2) to propose sustainable solutions to develop a beneficial microbiome to inhibit the onset of RS.
A Hollow-Shaped ZIF-8-N-Doped Porous Carbon Fiber for High-Performance Zn-Ion Hybrid Supercapacitors
Authors: Wei Mingqi , Jiang Zhenlong , Yang Chengcheng , Jiang Tao , Zhang Linlin , Zhao Guangzhen , Zhu Guang , Yu Lianghao , Zhu Yuanyuan
Published: August 2023

The advantages of low cost, high theoretical capacity, and dependable safety of aqueous zinc ion hybrid supercapacitors (ZHSCs) enable their promising use in flexible and wearable energy storage devices. However, achieving extended cycling stability in ZHSCs is still challenged by the limited availability of carbon cathode materials that can effectively pair with zinc anode materials. Here, we report a method for synthesising heteroatom-doped carbon nanofibers using electrostatic spinning and metal-organic frameworks (specifically ZIF-8). Assembled Zn//ZPCNF-1.5 ZHSCs exhibited 193 mA h g−1 specific capacity at 1 A g−1 and 162.6 Wh kg−1 energy density at 841.2 kW kg−1. Additionally, the device showed an ultra-long cycle life, maintaining 98% capacity after 20,000 cycles. Experimental analysis revealed an increase in the number of pores and active sites after adding ZIF-8 to the precursor. Furthermore, N doping effectively enhanced Zn2+ ions chemical adsorption and improved Zn-ion storage performance. This work provides a feasible design strategy to enhance ZHSC energy storage capability for practical applications.
Programmable Digital-Microfluidic Biochips for SARS-CoV-2 Detection
Authors: Wang Yuxin , Chan Yun-Sheng , Chae Matthew , Shi Donglu , Lee Chen-Yi , Diao Jiajie
Published: August 2023

Biochips, a novel technology in the field of biomolecular analysis, offer a promising alternative to conventional testing equipment. These chips integrate multiple functions within a single system, providing a compact and efficient solution for various testing needs. For biochips, a pattern-control micro-electrode-dot-array (MEDA) is a new, universally viable design that can replace microchannels and other micro-components. In a Micro Electrode Dot Array (MEDA), each electrode can be programmatically controlled or dynamically grouped, allowing a single chip to fulfill the diverse requirements of different tests. This capability not only enhances flexibility, but also contributes to cost reduction by eliminating the need for multiple specialized chips. In this paper, we present a visible biochip testing system for tracking the entire testing process in real time, and describe our application of the system to detect SARS-CoV-2.
Detecting Skin Reactions in Epicutaneous Patch Testing with Deep Learning: An Evaluation of Pre-Processing and Modality Performance
Authors: Vezakis Ioannis A. , Lambrou George I. , Kyritsi Aikaterini , Tagka Anna , Chatziioannou Argyro , Matsopoulos George K.
Published: August 2023

Epicutaneous patch testing is a well-established diagnostic method for identifying substances that may cause Allergic Contact Dermatitis (ACD), a common skin condition caused by exposure to environmental allergens. While the patch test remains the gold standard for identifying allergens, it is prone to observer bias and consumes valuable human resources. Deep learning models can be employed to address this challenge. In this study, we collected a dataset of 1579 multi-modal skin images from 200 patients using the Antera 3D® camera. We then investigated the feasibility of using a deep learning classifier for automating the identification of the allergens causing ACD. We propose a deep learning approach that utilizes a context-retaining pre-processing technique to improve the accuracy of the classifier. In addition, we find promise in the combination of the color image and false-color map of hemoglobin concentration to improve diagnostic accuracy. Our results showed that this approach can potentially achieve more than 86% recall and 94% specificity in identifying skin reactions, and contribute to faster and more accurate diagnosis while reducing clinician workload.
Systematic Screening Study for the Selection of Proper Stabilizers to Produce Physically Stable Canagliflozin Nanosuspension by Wet Milling Method
Authors: Pirincci Tok Yagmur , Mesut Burcu , Güngör Sevgi , Sarıkaya Ali Osman , Aldeniz Emre Erol , Dude Udaya , Özsoy Yıldız
Published: August 2023

One of the crucial approaches to managing the low solubility and weak bioavailability of drugs is via nanocrystal technology. Through this technology, drug particles have an increased solubility and a faster dissolution rate due to high surface free energy, which requires an appropriate stabilizer(s) to prevent instabilities during the manufacturing process and storage of the nanosuspension. This study aimed to establish a scientific predictive system for properly selecting stabilizers or to reduce the attempts on a trial-and-error basis in the wet-milling method. In total, 42 experiments were performed to examine the effect of critical material attributes on the wettability of the drug, the saturation solubility in the stabilizer solutions or combinations thereof and the dynamic viscosity of stabilizer solutions. All data were evaluated by Minitab 19® and an optimization study was performed. The optimized formulation at a certain concentration of stabilizer combination was ground by Dyno Mill® with 0.3 mm beads for one hour. The optimized nanosuspension with a particle size of 204.5 nm was obtained in short milling time and offered 3.05- and 3.51 times better dissolution rates than the marketed drug product (Invokana® 100 mg) in pH 4.5 and pH 6.8 as non-sink conditions, respectively. The formulation was monitored for three months at room temperature and 4 °C. The parameters were 261.30 nm, 0.163, −14.1 mV and 261.50 nm, 0.216 and −17.8 mV, respectively. It was concluded that this approach might indicate the appropriate selection of stabilizers for the wet-milling process.
Examination of Lower Limb Microcirculation in Diabetic Patients with and without Intermittent Claudication
Authors: Biró Katalin , Sándor Barbara , Tótsimon Kinga , Koltai Katalin , Fendrik Krisztina , Endrei Dóra , Vékási Judit , Tóth Kálmán , Késmárky Gábor
Published: August 2023

Intermittent claudication is a frequent complaint in lower extremity artery disease, but approximately two thirds of patients are asymptomatic, most of which are diabetic patients. Non-invasive angiological and microrheological tests on diabetic subjects with and without intermittent claudication were performed in the present study. In total, 98 diabetic patients were included and divided into two groups: 20 patients (63.5 ± 8.8 years, 55% men, 45% women) had intermittent claudication, 78 patients (65.5 ± 9.3 years, 61.5% men, 38.5% women) were asymptomatic. Hand-held Doppler ultrasound examination, transcutaneous tissue partial oxygen pressure (tcpO2) measurement, Rydel–Seiffer tuning fork tests, and 6-min walk tests were performed, and erythrocyte aggregation was investigated. Ankle–brachial index (p < 0.02) and tcpO2, measured during provocation tests (p < 0.003) and the 6-min walk test (p < 0.0001), significantly deteriorated in the symptomatic group. A higher erythrocyte aggregation index and faster aggregate formation was observed in claudication patients (p < 0.02). Despite the statistically better results of the asymptomatic group, 13% of these patients had severe limb ischemia based on the results of tcpO2 measurement. Claudication can be associated with worse hemodynamic and hemorheological conditions in diabetic patients; however, severe ischemia can also develop in asymptomatic subjects. Non-invasive vascular tests can detect ischemia, which highlights the importance of early instrumental screening of the lower limbs.
Immunomorphogenesis in Degenerative Disc Disease: The Role of Proinflammatory Cytokines and Angiogenesis Factors
Authors: Pravdyuk Natalya G. , Novikova Anna V. , Shostak Nadezhda A. , Buianova Anastasiia A. , Tairova Raisa T. , Patsap Olga I. , Raksha Aleksandr P. , Timofeyev Vitaliy T. , Feniksov Victor M. , Nikolayev Dmitriy A. , Senko Ilya V.
Published: August 2023

Back pain (BP) due to degenerative disc disease (DDD) is a severe, often disabling condition. The aim of this study was to determine the association between the expression level of proinflammatory cytokines (IL-1β, IL-6, and IL-17), angiogenesis markers (VEGF-A and CD31) in intervertebral disc (IVD) tissue and IVD degeneration in young people with discogenic BP. In patients who underwent discectomy for a disc herniation, a clinical examination, magnetic resonance imaging of the lumbar spine, histological and immunohistochemical analyses of these factors in IVD were performed in comparison with the parameters of healthy group samples (controls). Histology image analysis of IVD fragments of the DDD group detected zones of inflammatory infiltration, combined with vascularization, the presence of granulation tissue and clusters of chondrocytes in the tissue of nucleus pulposus (NP). Statistically significant increased expression of IL-1β, IL-6, IL-17, VEGF-A and CD31 was evident in the samples of the DDD group compared with the controls, that showed a strong correlation with the histological disc degeneration stage. Our results denote an immunoinflammatory potential of chondrocytes and demonstrates their altered morphogenetic properties, also NP cells may trigger the angiogenesis.
New Onset and Exacerbation of Psoriasis Following COVID-19 Vaccination: A Review of the Current Knowledge
Authors: Potestio Luca , Battista Teresa , Cacciapuoti Sara , Ruggiero Angelo , Martora Fabrizio , Fornaro Luigi , Camela Elisa , Megna Matteo
Published: August 2023

COVID-19 vaccination was the main measure to overcome the pandemic. As with other drugs and vaccines, mild to moderate adverse events have been reported following vaccination. In addition, several cutaneous reactions have been described. In particular, there are several reports investigating de novo psoriasis or the exacerbation of psoriasis following COVID-19 vaccination. However, data on the possible pathogenetic mechanisms as well as comprehensive manuscripts on the topic are scant. Thus, the aim of our manuscript was to perform a review of the current literature on post-COVID-19 vaccination exacerbations and new-onset psoriasis in order to offer a wide perspective on this area and to point out possible pathogenetic mechanisms. Research on the current literature was performed following PRISMA guidelines. In total, 49 studies involving 134 patients developing new-onset psoriasis (n = 27, 20.1%) or psoriasis exacerbation (n = 107, 79.9%) were collected. Although cases of de novo psoriasis or a worsening of psoriasis have been reported following vaccination, all of the cases have been successfully treated while overall benefit–risk profile of COVID-19 vaccination does not justify vaccine hesitancy due to the risk of psoriasis being developed or worsening. Certainly, further studies are needed to identify possible pathogenetic mechanisms in order to identify “at-risk” patients. Finally, vaccination should not be discouraged.
Growth Efficiency of Chlorella sorokiniana in Synthetic Media and Unsterilized Domestic Wastewater
Authors: Bulynina Svetlana S. , Ziganshina Elvira E. , Ziganshin Ayrat M.
Published: August 2023

Incorporating a variety of microalgae into wastewater treatment is considered an economically viable and environmentally sound strategy. The present work assessed the growth characteristics of Chlorella sorokiniana during cultivation in balanced synthetic media and domestic wastewater. Increasing the NH4+–N concentration to 360 mg L−1 and adding extra PO43−–P and SO42−–S (up to 80 and 36 mg L−1, respectively) contributed to an increase in the total biomass levels (5.7–5.9 g L−1) during the cultivation of C. sorokiniana in synthetic media. Under these conditions, the maximum concentrations of chlorophylls and carotenoids were 180 ± 7.5 and 26 ± 1.4 mg L−1, respectively. Furthermore, when studying three types of domestic wastewaters, it was noted that only one wastewater contributed to the productive growth of C. sorokiniana, but all wastewaters stimulated an increased accumulation of protein. Finally, the alga, when growing in optimal unsterilized wastewater, showed a maximum specific growth rate of 0.73 day−1, a biomass productivity of 0.21 g L−1 day−1, and 100% NH4+–N removal. These results demonstrate that the tested alga actively adapts to changes in the composition of the growth medium and accumulates high levels of protein in systems with poor-quality water.
Effects of Repetitive Transcranial Magnetic Stimulation at the Cerebellum on Working Memory
Authors: Yao Jiangnan , Song Bo , Shi Jingping , Yin Kuiying , Du Wentao
Published: August 2023

Transcranial magnetic stimulation is a widely used brain intervention technique in clinical settings. In recent years, the role of the cerebellum in learning and memory has become one of the hotspots in the field of cognitive neuroscience. In this study, we recruited 36 healthy college or graduate students as subjects and divided them into groups, with 10 to 14 subjects in each group. We performed 5 Hz and 20 Hz repeated transcranial magnetic stimulation and sham stimulation on the Crus II subregion of the cerebellum in different groups, then let them complete the 2-back working memory task before and after the stimulation. We simultaneously recorded the electroencephalogram in the experiment and analyzed the data. We found that after repeated transcranial magnetic stimulation of the cerebellum at 5 Hz and 20 Hz, the N170 and P300 event-related potential components in the prefrontal cortex showed significant differences compared to those in the sham stimulation group. Using phase-locked values to construct brain networks and conduct further analysis, we discovered that stimulation frequencies of 5 Hz and 20 Hz had significant effects on the local and global efficiency of brain networks in comparison to the sham stimulation group. The results showed that repeated transcranial magnetic stimulation on cerebellar targets can effectively affect the subjects’ working memory tasks. Repeated transcranial magnetic stimulation at 5 Hz and 20 Hz could enhance the excitatory responses of the frontal lobes. After stimulation at 5 Hz and 20 Hz, the efficiency of the brain network significantly improved.
Survival after Pure (Acute) Erythroid Leukemia in the United States: A SEER-Based Study
Authors: Gera Kriti , Martir Daniela , Xue Wei , Wingard John R.
Published: August 2023

Background: Acute erythroid leukemia (AEL), also known as pure erythroid leukemia, is a rare subtype of acute myeloid leukemia (AML) characterized by the proliferation of malignant erythroid precursors. Outcome data at the population level are scarce. Methods: We performed a retrospective analysis of the Surveillance Epidemiology and End Results (SEER) database. All cases with a histologically confirmed diagnosis of acute (pure) erythroid leukemia during the period of 2000–2019 were included in the study. The Kaplan–Meier method was used to perform survival analysis. The significance of differences between overall survival (OS) was analyzed using the log-rank test. Results: In total, 968 patients were included in the study. The median age was 68 years (range 0–95), 62% of patients were males, and 62.5% (n = 605) were treated with chemotherapy. The median OS for <18, 18–49, 50–64, 65–79 and 80+ age groups was 69, 18, 8, 3 and 1 month, respectively (p < 0.0001). Patients who received chemotherapy had significantly improved OS compared to patients who did not, among both adults (p < 0.0001) and children (p = 0.004). There were no significant differences in OS based on sex, race, ethnicity and median household income. Median OS for adults diagnosed in 2000–2004, 2005–2009, 2010–2014, 2015–2019 was 4, 6, 6 and 3 months, respectively, with no significant differences in OS between these groups. Conclusion: AEL occurs in all age groups but is most common in the elderly. Outcomes are poor with current chemotherapeutic agents, with no improvement in the last two decades. This study stresses the urgent need for investigational agents.
Intraoperative Neuromonitoring of the Visual Pathway in Asleep Neuro-Oncology Surgery
Authors: Soumpasis Christos , Díaz-Baamonde Alba , Ghimire Prajwal , Baig Mirza Asfand , Borri Marco , Jarosz Josef , Gullan Richard , Ashkan Keyoumars , Bhangoo Ranjeev , Vergani Francesco , Lavrador Jose Pedro , Mirallave Pescador Ana
Published: August 2023

Brain tumour surgery in visual eloquent areas poses significant challenges to neurosurgeons and has reported inconsistent results. This is a single-centre prospective cohort study of patients admitted for asleep surgery of intra-axial lesions in visual eloquent areas. Demographic and clinical information, data from tractography and visual evoked potentials (VEPs) monitoring were recorded and correlated with visual outcomes. Thirty-nine patients were included (20 females, 19 males; mean age 52.51 ± 14.08 years). Diffuse intrinsic glioma was noted in 61.54% of patients. There was even distribution between the temporal, occipital and parietal lobes, while 55.26% were right hemispheric lesions. Postoperatively, 74.4% remained stable in terms of visual function, 23.1% deteriorated and 2.6% improved. The tumour infiltration of the optic radiation on tractography was significantly related to the visual field deficit after surgery (p = 0.016). Higher N75 (p = 0.036) and P100 (p = 0.023) amplitudes at closure on direct cortical VEP recordings were associated with no new postoperative visual deficit. A threshold of 40% deterioration of the N75 (p = 0.035) and P100 (p = 0.020) amplitudes correlated with a risk of visual field deterioration. To conclude, direct cortical VEP recordings demonstrated a strong correlation with visual outcomes, contrary to transcranial recordings. Invasion of the optic radiation is related to worse visual field outcomes.
Validated Pretreatment Prediction Models for Response to Neoadjuvant Therapy in Patients with Rectal Cancer: A Systematic Review and Critical Appraisal
Authors: Tanaka Max D. , Geubels Barbara M. , Grotenhuis Brechtje A. , Marijnen Corrie A. M. , Peters Femke P. , van der Mierden Stevie , Maas Monique , Couwenberg Alice M.
Published: August 2023

Pretreatment response prediction is crucial to select those patients with rectal cancer who will benefit from organ preservation strategies following (intensified) neoadjuvant therapy and to avoid unnecessary toxicity in those who will not. The combination of individual predictors in multivariable prediction models might improve predictive accuracy. The aim of this systematic review was to summarize and critically appraise validated pretreatment prediction models (other than radiomics-based models or image-based deep learning models) for response to neoadjuvant therapy in patients with rectal cancer and provide evidence-based recommendations for future research. MEDLINE via Ovid, Embase.com, and Scopus were searched for eligible studies published up to November 2022. A total of 5006 studies were screened and 16 were included for data extraction and risk of bias assessment using Prediction model Risk Of Bias Assessment Tool (PROBAST). All selected models were unique and grouped into five predictor categories: clinical, combined, genetics, metabolites, and pathology. Studies generally included patients with intermediate or advanced tumor stages who were treated with neoadjuvant chemoradiotherapy. Evaluated outcomes were pathological complete response and pathological tumor response. All studies were considered to have a high risk of bias and none of the models were externally validated in an independent study. Discriminative performances, estimated with the area under the curve (AUC), ranged per predictor category from 0.60 to 0.70 (clinical), 0.78 to 0.81 (combined), 0.66 to 0.91 (genetics), 0.54 to 0.80 (metabolites), and 0.71 to 0.91 (pathology). Model calibration outcomes were reported in five studies. Two collagen feature-based models showed the best predictive performance (AUCs 0.83–0.91 and good calibration). In conclusion, some pretreatment models for response prediction in rectal cancer show encouraging predictive potential but, given the high risk of bias in these studies, their value should be evaluated in future, well-designed studies.
The Efficacy of CB-103, a First-in-Class Transcriptional Notch Inhibitor, in Preclinical Models of Breast Cancer
Authors: Vigolo Michele , Urech Charlotte , Lamy Sebastien , Monticone Giulia , Zabaleta Jovanny , Hossain Fokhrul , Wyczechowska Dorota , Del Valle Luis , O’Regan Ruth M. , Miele Lucio , Lehal Rajwinder , Majumder Samarpan
Published: August 2023

Background: The efficacy of CB-103 was evaluated in preclinical models of both ER+ and TNBC. Furthermore, the therapeutic efficacy of combining CB-103 with fulvestrant in ER+ BC and paclitaxel in TNBC was determined. Methods: CB-103 was screened in combination with a panel of anti-neoplastic drugs. We evaluated the anti-tumor activity of CB-103 with fulvestrant in ESR1-mutant (Y537S), endocrine-resistant BC xenografts. In the same model, we examined anti-CSC activity in mammosphere formation assays for CB-103 alone or in combination with fulvestrant or palbociclib. We also evaluated the effect of CB-103 plus paclitaxel on primary tumors and CSC in a GSI-resistant TNBC model HCC1187. Comparisons between groups were performed with a two-sided unpaired Students’ t-test. A one-way or two-way ANOVA followed by Tukey’s post-analysis was performed to analyze the in vivo efficacy study results. The results: CB-103 showed synergism with fulvestrant in ER+ cells and paclitaxel in TNBC cells. CB-103 combined with fulvestrant or paclitaxel potently inhibited mammosphere formation in both models. Combination of CB-103 and fulvestrant significantly reduced tumor volume in an ESR1-mutant, the endocrine-resistant BC model. In a GSI-resistant TNBC model, CB-103 plus paclitaxel significantly delayed tumor growth compared to paclitaxel alone. Conclusion: our data indicate that CB-103 is an attractive candidate for clinical investigation in endocrine-resistant, recurrent breast cancers with biomarker-confirmed Notch activity in combination with SERDs and/or CDKis and in TNBCs with biomarker-confirmed Notch activity in combination with taxane-containing chemotherapy regimens.
RXR Agonists Enhance Lenalidomide Anti-Myeloma Activity and T Cell Functions while Retaining Glucose-Lowering Effect
Authors: Wu Jian , Wang Xiaobei , Zhang Min , Mathews Parker , Kang Yubin
Published: August 2023

Retinoid X receptor (RXR) heterodimerizes with the PPAR nuclear hormone receptor and regulates its downstream events. We investigated the effects of RXR agonists (LG100754, bexarotene, AGN194204, and LG101506) on lenalidomide’s anti-myeloma activity, T cell functions, and the level of glucose and lipids in vivo. Genetic overexpression and CRISPR/Cas9 knockout experiments were conducted in multiple myeloma (MM) cell lines and Jurkat T cell lines to determine the roles of CRBN in RXR-agonist mediated effects. A xenograft mouse model of MM was established to determine the combination effect of LG100754 and lenalidomide. The combination of RXR agonists and lenalidomide demonstrated synergistic activity in increasing CRBN expression and killing myeloma cells. Mechanistically, the RXR agonists reduced the binding of PPARs to the CRBN promoter, thereby relieving the repressor effect of PPARs on CRBN transcription. RXR agonists downregulated the exhaustion markers and increased the activation markers of Jurkat T cells and primary human T cells. Co-administration of LG100754 and lenalidomide showed enhanced anti-tumor activity in vivo. LG100754 retained its glucose- and lipid-lowering effects. RXR agonists demonstrate potential utility in enhancing drug sensitivity and T-cell function in the treatment of myeloma.
Optical Immunosensors for Bacteria Detection in Food Matrices
Authors: Kourti Dimitra , Angelopoulou Michailia , Petrou Panagiota , Kakabakos Sotirios
Published: August 2023

Optical immunosensors are one of the most popular categories of immunosensors with applications in many fields including diagnostics and environmental and food analysis. The latter field is of particular interest not only for scientists but also for regulatory authorities and the public since food is essential for life but can also be the source of many health problems. In this context, the current review aims to provide an overview of the different types of optical immunosensors focusing on their application for the determination of pathogenic bacteria in food samples. The optical immunosensors discussed include sensors based on evanescent wave transduction principles including surface plasmon resonance (SPR), fiber-optic-, interferometric-, grating-coupler-, and ring-resonator-based sensors, as well as reflectometric, photoluminescence, and immunosensors based on surface-enhanced Raman scattering (SERS). Thus, after a short description of each transduction technique, its implementation for the immunochemical determination of bacteria is discussed. Finally, a short commentary about the future trends in optical immunosensors for food safety applications is provided.
Ratio-Metric Fluorescence/Colorimetric and Smartphone-Assisted Visualization for the Detection of Dopamine Based on Cu-MOF with Catecholase-like Activity
Authors: Guo Yushuang , Chen Mingli , Yang Ting , Wang Jianhua
Published: August 2023

As the most abundant catecholamine neurotransmitter in the brain, dopamine plays an important role in the normal physiological process, and its level in urine also changes during human pathological processes. In clinic, the detection of dopamine in urine is a potential marker for the diagnosis and the treatment of endocrine-related diseases. In this work, a copper metal organic framework with catecholase-like activity was prepared via the precipitation of Cu2+ and imidazole, simulating the N-Cu coordination environment in the active site of catecholase. Cu-MOF (the copper–metal organic framework) can catalyze the oxidation of DA (dopamine) to dopaquinone using O2 in the air. The oxidation product can further react with 1,3-dihydroxynaphthalene to produce a fluorophore product. Based on the above reaction, a multimodal sensing platform with three signal outputs, including ratio-metric fluorescence, absorbance and digital information extracted from smartphone images for simple and sensitive determination of DA, was proposed, with detection limits of 0.0679, 0.3206, and 0.3718 μM, respectively. This multimodal sensing platform was able to detect DA in body fluid in a self-correcting way, as demonstrated by the successful determination of DA in normal human urine samples, and samples with a high level of interference.