bluuebunny/crossref_metadata_2025 huggingface dataset:
The Butterfly Effect: Mild Soil Pollution with Heavy Metals Elicits Major Biological Consequences in Cobalt-Sensitized Broad Bean Model Plants
Authors: Šiukšta Raimondas , Pukenytė Vėjūnė , Kleizaitė Violeta , Bondzinskaitė Skaistė , Čėsnienė Tatjana
Published: April 2022

Among the heavy metals (HMs), only cobalt induces a polymorphic response in Vicia faba plants, manifesting as chlorophyll morphoses and a ‘break-through’ effect resulting in the elevated accumulation of other HMs, which makes Co-pretreated broad bean plants an attractive model for investigating soil pollution by HMs. In this study, Co-sensitized V. faba plants were used to evaluate the long-term effect of residual industrial pollution by examining biochemical (H2O2, ascorbic acid, malondialdehyde, free proline, flavonoid, polyphenols, chlorophylls, carotenoids, superoxide dismutase) and molecular (conserved DNA-derived polymorphism and transcript-derived polymorphic fragments) markers after long-term exposure. HM-polluted soil induced a significantly higher frequency of chlorophyll morphoses and lower levels of nonenzymatic antioxidants in Co-pretreated V. faba plants. Both molecular markers effectively differentiated plants from polluted and control soils into distinct clusters, showing that HMs in mildly polluted soil are capable of inducing changes in DNA coding regions. These findings illustrate that strong background abiotic stressors (pretreatment with Co) can aid investigations of mild stressors (slight levels of soil pollution) by complementing each other in antioxidant content reduction and induction of DNA changes.
Preparation and Properties of SBS/REOB-Modified Rejuvenated Asphalt
Authors: Cui Yanchen , Li Jin , Zhu Li , Zuo Shen , Yu Miaozhang , Liu Peng , Cui Xinzhuang
Published: April 2022

REOB (Recycled Engine Oil Bottom) rejuvenated asphalt can solve the problems of environmental pollution and resource waste. In order to improve the performance of REOB rejuvenated asphalt, an SBS modifier was applied. An orthogonal test was carried out to optimize the preparation scheme of SBS/REOB-modified rejuvenated asphalt, and the physical rheological properties and microscopic mechanisms of modified rejuvenated asphalt with different SBS and REOB dosages were studied. The test results show that the optimal mixture of the SBS/REOB-modified and rejuvenated asphalt is 9% REOB + 4.5% SBS, the optimal shear time is 40 min, and the shear rate is 5000 r/min. SBS modification of the REOB rejuvenated asphalt can obtain the same excellent high temperature performance as ordinary SBS-modified asphalt, and the modification process can greatly improve the high-temperature deformation resistance of rejuvenated asphalt. After SBS modification of the REOB rejuvenated asphalt, its S value is significantly lower than the base asphalt and the REOB rejuvenated asphalt, and its m value is also greatly improved compared with the base asphalt and the REOB rejuvenated asphalt, indicating that the low-temperature performance of the modified rejuvenated asphalt is greatly improved compared with the rejuvenated asphalt and is better than the base asphalt. The fluorescence microscopic test revealed that both the modified rejuvenated asphalt and the SBS-modified new asphalt prepared in the laboratory showed a continuous asphalt phase, and the SBS phase of the modified rejuvenated asphalt could basically reach the level of finished modified asphalt.
Metal Release Mechanism and Electrochemical Properties of Lix(Ni1/3Mn1/3Co1/3)O2
Authors: Hudson Blake G. , Mason Sara E.
Published: April 2022

Complex metal oxides (CMOs) are used broadly in applications including electroreactive forms found in lithium-ion battery technology. Computational chemistry can provide unique information about how the properties of CMO cathode materials change in response to changes in stoichiometry, for example, changes of the lithium (Li) content during the charge–discharge cycle of the battery. However, this is difficult to measure experimentally due to the small cross-sectional area of the cations. Outside of operational conditions, the Li content can influence the transformations of the CMO when exposed to the environment. For example, metal release from CMOs in aqueous settings has been identified as a cross-cutting mechanism important to CMO degradation. Computational studies investigating metal release from CMOs show that the thermodynamics depend on the oxidation states of lattice cations, which is expected to vary with the lithium content. In this work, computational studies track changes in metal release trends as a function of Li content in Lix(Ni1/3Mn1/3Co1/3)O2 (NMC). The resulting dataset is used to construct a random forest tree (RFT) machine learning (ML) model. A modeling challenge in delithiation studies is the large configurational space to sample. Through investigating multiple configurations at each lithium fraction, we find structural features associated with favorable energies to chemically guide the identification of relevant structures and adequately predict voltage values.
Experimental Investigation and Modelling of the Droplet Size in a DN300 Stirred Vessel at High Disperse Phase Content Using a Telecentric Shadowgraphic Probe
Authors: Wirz Dominic , Friebel Anne , Rave Kevin , Hermes Mario , Skoda Romuald , von Harbou Erik , Bart Hans-Jörg
Published: April 2022

In this work, steady-state droplet size distributions in a DN300 stirred batch vessel with a Rushton turbine impeller are investigated using an insertion probe based on the telecentric transmitted light principle. High-resolution droplet size distributions are extracted from the images using a convolutional neural network for image-analysis in order to investigate the influence of impeller speed and phase fraction (up to 50 vol.-%). In addition, Sauter mean diameters were calculated and correlated with two semi-empirical approaches, while the standard approach only accomplished 5.7% accuracy, and the correlation of Laso et al. provided a relative mean error of 4.0%. In addition, the correlated exponent in the Weber number was fitted to the experimental data of this work yielding a slightly different value than the theoretical (−0.6), which allows a better representation of the low coalescence tendency of the system, which is usually neglected in standard procedures.
The Association between Influenza Vaccination and Stroke Risk in Patients with Hypertension: A Nationwide Population-Based Study
Authors: Lin Cheng-Hsin , Chiu Chun-Chih , Yang Tsung-Yeh , Fang Yu-Ann , Lei Meng-Huan , Yeh Hsien-Tang , Chen Chun-Chao , Hao Wen-Rui , Kuo Chung-Hsien , Liu Ju-Chi
Published: April 2022

There is evidence of strong association between influenza infections and stroke; however, the influenza vaccination and its effect on strokes is currently unclear. In the present study, Taiwan’s National Health Insurance Database was used in obtaining data for study subjects 55 years and older diagnosed with hypertension (n = 59,251; 25,266 vaccinated and 33,985 unvaccinated subjects) from 2001–2012. Propensity scores were calculated using a logistic regression model to determine the effects of vaccination by accounting for covariates that predict receiving the intervention (vaccine). A time-dependent Cox proportional hazard model was used to calculate the hazard ratios (HRs) for stroke in vaccinated and unvaccinated patients. Influenza vaccination was associated with a 42%, 40% and 44% stroke risk reduction in the entire cohort for all seasons, the influenza season and the non-influenza season, respectively (Adjust hazard ratio [aHR]: 0.58, 95% confidence interval [CI]: 0.56–0.61; aHR: 0.60, 95% CI: 0.56–0.63; aHR: 0.56, 95% CI: 0.52–0.60, for all seasons, the influenza season and the non-influenza season, respectively). The effect of risk reduction by vaccination also revealed a trend of dose dependency. Among subjects between 55 to 64 years old with four or more vaccinations during the study period, there is a 73% risk reduction for stroke during the non-influenza season (aHR: 0.27, 95% CI: 0.20–0.34). In conclusion, the influenza vaccination exerts dose-dependent and synergistic protective effects against stroke in individuals 55 years and older with hypertension.
Classifying Malicious Documents on the Basis of Plain-Text Features: Problem, Solution, and Experiences
Authors: Hong Jiwon , Jeong Dongho , Kim Sang-Wook
Published: April 2022

Cyberattacks widely occur by using malicious documents. A malicious document is an electronic document containing malicious codes along with some plain-text data that is human-readable. In this paper, we propose a novel framework that takes advantage of such plaintext data to determine whether a given document is malicious. We extracted plaintext features from the corpus of electronic documents and utilized them to train a classification model for detecting malicious documents. Our extensive experimental results with different combinations of three well-known vectorization strategies and three popular classification methods on five types of electronic documents demonstrate that our framework provides high prediction accuracy in detecting malicious documents.
Dynamic Analysis on the Parametric Resonance of the Tower–Multicable–Beam Coupled System
Authors: He Shuanhai , Chen Kefan , Song Yifan , Wang Binxian , Wang Kang , Hou Wei
Published: April 2022

Considering the effect of the bridge deck’s bending stiffness and the indirect effect of adjacent cables (CEB), this paper aims to propose a refined model to reliably analyze the complex internal resonance mechanism of the tower–multicable–beam coupled system (MCS) under nonlinear geometric conditions. To accurately analyze the dynamic behavior, the shear difference effect is applied to simulate the continuous rigidity of the single beam. The dynamic equations of the whole resonance system are derived based on the D’Alembert Principle and the Finite Difference Method, the Galerkin Method and verified by the case study. The results of the numerical simulation based on the Fourth Runge–Kutta Method show that the dynamic parameter of each component is closely related to the coupled resonance of the system. The dynamic behavior under two conditions, tower–cable 1:1 resonance (TCR) or cable–beam 1:2 resonance (CBR), is deeply analyzed. Additionally, the excitation effect of the maximum amplitude by two excitation approaches, the initial displacement or initial velocity, both show a linear increase. The mutual transmission process of vibration excitation on the cable through the bridge beam or the tower as the medium is also further discussed.
Interaction between Rockfalls and Vehicles Studied for Speed Limit Zonation along Mountainous Roads
Authors: Pappalardo Giovanna , Caliò Davide , Mineo Simone
Published: April 2022

A rockfall risk assessment along the transportation route to a historical village is presented herein with the aim of evaluating the potential of this approach for speed limit zonation. Mountainous roads are often subject to rockfalls, which should be taken into account for territorial management and planning, especially when dealing with dynamic variables, such as vehicular traffic. Rockfall risk analysis along roads is often aimed at assessing a risk value to plan or prioritize mitigation purposes. Nevertheless, such approaches can also be used to regulate traffic in terms of posted speed limits. Traffic is, indeed, a key variable in rockfall risk analysis due to the spatial and temporal correspondence that a vehicle can have with an either falling or fallen rock block. In order to address this relationship for speed limit zonation purposes, in this paper, a semi-quantitative Rockfall Hazard Rating System was applied to a mountainous road leading to a popular tourist destination in eastern Sicily (Italy), which is characterized by winding paths. This approach, which was chosen for its feasibility and international diffusion, was repeated by taking five different vehicle speed scenarios into account, thus providing an innovative application of the procedure in terms of aims and practical results. The achieved outcomes were used to draft thematic maps, as well as to define a suitable speed limit zonation related to the rockfall risk, highlighting that the road visibility strongly affects the final results. The achieved outcomes demonstrate how a scientific approach can be turned into a practical tool of broad utility, especially in mountainous settings, where winding roads and rockfall problems often condition the viability.
The Effect of the Water Tower of Typhoon Mangkhut (2018)
Authors: Zuo Haosheng , Chen Yilun , Chen Shumin , Li Weibiao , Zhang Aoqi
Published: April 2022

On 16 September 2018, the Yangtze River Delta (YRD) experienced heavy precipitation, with the local daily precipitation exceeding 250 mm. Using ERA5 reanalysis data and satellite observations from the GPM, we review this heavy rain event in terms of its meteorological triggers and water vapor transport. As the high-level water vapor produced by Typhoon Mangkhut continued to be transported northward, the precipitation in the YRD gradually increased, and stratus precipitation played a major role in this event. The high-level water vapor continued to be transported northward to the east of Taiwan Island without falling, so heavy precipitation did not appear to the east of Taiwan Island. In the present study, we suggest that the meteorological trigger of this event was mainly the gradual falling of ice particles moving northward from a high altitude. The high-level ice particles originated from the “water tower” at the center of Typhoon Mangkhut, which pumped low-level water vapor into the high-level water vapor. In general, the appearance of abnormal values of high-level water vapor transport is an important atmospheric disturbance related to heavy precipitation in the downstream areas of high-level wind, and the typhoon water tower can be used as an important forecast signal for long-distance heavy precipitation in China during the active typhoon period.
Quantifying Sustainable Land-Water-Energy-Food Nexus: The Case of Sustainable Livelihoods in an East African Rift Valley
Authors: Wolde Zinabu , Wu Wei , Ketema Haile , Karikari Benjamin , Liu Xiansheng
Published: April 2022

The sustainable management of Land-Water-Energy-Food (LWEF) nexus requires an environmental characterization that allows the comparison of complex interlinkages between nexus resources and livelihoods. This complexity makes this characterization difficult coupled with limited study in quantifying sustainability of LWEF nexus and its linkage with livelihood. Therefore, the present study aimed to investigate the link between sustainable LWEF nexus and livelihoods. In order to address the objective the proposed methodology starts with a detailed identification of LWEF and livelihood indicators which depicts well-defined, shared, and holistic methods to evaluate sustainability. With this we used analytical hierarchy process and pair wise comparison matrix in combination with weighting model. The result of composite LWEF nexus index was 0.083 representing, low sustainability. Besides, this composite index implies the use and management of LWEF nexus resources in the study area is very low, as the composite index approach to 1, the use and management of nexus resources are in a good condition which characterized by sustainability. This could be linked with nexus resources consumption, use, and management. From the analysis of the weight of land, water, energy and food nexus resources, the highest weight was observed for food. The focus of on food production only shows no clear synergy on provisioning, supporting or regulating nexus resources to address livelihoods. The result further showed that LWEF nexus resources have strong correlation with livelihoods. This was evidenced by social (r > 0.8, p < 0.01), natural (r > 0.3, p < 0.05) and physical (r > 0.6, p < 0.01) livelihood indicators showed strong positive correlation with LWEF nexus resources. Based on the finding of the study, it was observed that managing nexus resources not only provide a significant contribution to achieve sustainable LWEF nexus, but also be effective for enhancing livelihood through food security. This could be attained by strong evidence based policy to ensure sustainable use of nexus resources. The results provided by this study would serve as the foundation for future study, policy formulation and implementation.
Improving the Estimation of Throughfall Amounts in Primeval Forests along an Elevation Gradient on Mountain Gongga, Southwest China
Authors: Yang Ruxin , Wang Genxu , Cui Junfang , Guo Li , Wang Fei , Tang Xiangyu
Published: April 2022

Differences in rainfall partition into throughfall among different primeval forests distributed along an altitude gradient are inadequately investigated and understood. Through continuous and automatic monitoring of natural rainfall and throughfall along an elevation gradient on Mountain Gongga, we examined the response of throughfall to various rainfall patterns in the broadleaved forest (BF), broadleaved-coniferous mixed forest (MF), and coniferous forest (CF) across individual rain events from May to October in 2019. A series of linear models that estimate throughfall amount were obtained and compared. Results showed that throughfall was jointly controlled by rainfall characteristics (including amount, duration, average, and peak intensity) and leaf area index (LAI). Rainfall amount was the primary control for throughfall amount. The models with all rainfall parameters and LAI as variables did not markedly outperform (R2 enhancement by 0–0.02) the simple linear models with rainfall amount as the only variable; therefore, the latter are recommended due to simplicity and easiness of use. Although the correlation of throughfall with LAI was less prominent compared to rainfall parameters, LAI showed a significant positive linear correlation (p < 0.05) with the estimated rainfall amount threshold (the rainfall required to saturate the canopy) by the single-variable linear models at the monthly scale. Over the study period, penetration proportions of rainfall in BF, MF, and CF were 83%, 75%, and 80%, respectively. The rainfall amount threshold in CF (0.70 mm) was less than those in BF (0.80 mm) and MF (0.92 mm). Rain events of higher intensity exhibited a higher mean penetration proportion than lower intensity rain events. The use of single-variable linear models developed here, despite some overestimations of throughfall amount, could lead to an overall satisfactory estimation of rainfall redistribution in mountainous areas.
Impact of the Levels of COVID-19 Pandemic Prevention and Control Measures on Air Quality: A Case Study of Jiangsu Province, China
Authors: Ai Wenwen , Yang Xixi , Liu Duanyang , Zhang Min , Sun Yan , Wang Boni , Luo Xiaochun
Published: April 2022

In order to control the spread of the COVID-19 pandemic, the prevention and control measures of public health emergencies were initiated in all provinces of China in early 2020, which had a certain impact on air quality. In this study, taking Jiangsu Province in China as an example, the air pollution levels in different regions under different levels of pandemic prevention and control (PPC) measures are evaluated. The implementation of the prevention and control policies of COVID-19 pandemic directly affected the concentration of air pollutants. No matter what level of PPC measures was implemented, the air quality index (AQI) and pollutant concentrations of NO2, CO, PM10 and PM2.5 were all reduced by varied degrees. The higher the level of PPC measures, the greater the reduction was in air pollutant concentrations. Specifically, NO2 was the most sensitive to PPC policies. The concentrations of CO and atmospheric particulate matter (PM10 and PM2.5) decreased most obviously under the first and second level of PPC. The response speed of air quality to different levels of PPC measures varied greatly among different cities. Southern Jiangsu, which has a higher level of economic development and is dominated by secondary and tertiary industries, had a faster response speed and a stronger responsiveness. The results of this study reflect the economic vitality of different cities in economically advanced regions (i.e., Jiangsu Province) in China. Furthermore, the results can provide references for the formulation of PPC policies and help the government make more scientific and reasonable strategies for air pollution prevention and control.
Application of the Multi-Criteria Optimization Method to Repair Landslides with Additional Soil Collapse
Authors: Gvozdović Nikola , Božić-Tomić Kristina , Marković Ljubo , Marković Ljiljana Milić , Koprivica Suzana , Kovačević Miljan , Jovic Srdjan
Published: April 2022

In current practice, the remediation of landslides has shown that the biggest problem is the increase in the number of works, and therefore the price of the works. This is due to several factors, including characteristic of the soil, such as the collapse (collapse) of the surrounding ground around the main slide during landslide remediation. Unless these soil erosion effects are taken into account, recovery costs will overrun, which can jeopardize the planned budget. This paper presents a multi-criteria optimization of landslide remediation using the PROMETHEE method and determines the optional number of walls for the additional soil erosion. In a case study on examples of real landslides in the Republic of Serbia, the application of the method is presented and appropriate conclusions are drawn.
The Healthy Eater’s Idea and Related Behavior of a Healthy Diet—A Case Study with Kombucha Drinkers
Authors: Kim Juyoung , Bhattarai Uttam , Adhikari Koushik
Published: April 2022

Consumers’ demand for ‘healthy’ food is growing at a fast pace. Beverages are one of the fastest-growing segments in the healthy food market. Kombucha is a product with a rapid market growth and is considered a ‘healthy beverage’. Kombucha drinkers (n = 296) in the contiguous United States (U.S.) were targeted to investigate differences in the various consumer segments regarding the healthy eater’s behavior, namely—(1) healthy diet stage-of-change attitudes, (2) healthy eating criteria, and (3) label information usage. An online survey questionnaire was used as a data collection tool. The data were analyzed via chi-square tests and multiple correspondence analysis (MCA). The majority of kombucha consumers were health-conscious and kombucha fitted into their view of a healthy diet. The female, older, western U.S. residents, and higher-educated consumers were more likely to take action and maintain a healthier diet. Healthy eaters’ idea of a healthy diet was very similar to that of the general population. Kombucha consumers focused mainly on sugar/calories and natural/organic ingredients. Additional investigations to expand the understanding of the healthy eater’s behavior with actual kombucha samples are needed in the future.
COVID-19: A Systematic Review of the Transmissibility, Pathogenesis, Entry Factors, and Signature Immune Response
Authors: Fayyad Deena , Kelts Jessica L. , Nielson Tristan H. , Epelle Ibiere Lovelyn , Monear Nicodemus C. , Strawn Miguel T. G. , Woerner Benjamin N. , Xhabija Besa
Published: April 2022

Objectives: The emergence of coronavirus disease 2019 (COVID-19), caused by the novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to a global health calamity unprecedented in the modern world. The disease spread worldwide, and to date, there have been over 230 million confirmed cases of COVID-19, including approximately 4.7 million deaths. Mutant variants of the virus have raised concerns about additional pandemic waves and threaten to reverse our progress thus far to limit the spread of the virus. These variants include Alpha, Beta, and Delta (first reported in December 2020 in the United Kingdom, South Africa, and India, respectively) and Gamma (reported in January 2021 in Brazil). In some cases, countries have even reported a rise in daily cases higher than the first wave in March 2020. Given the rapidly evolving nature of COVID-19 and subsequent new findings and updates each day, this review article aims to comprehensively summarize the etiology, pathophysiology, and clinical features of SARS-CoV-2 infection. Methods: A systematic review of the literature was performed in accordance with PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines to gain insight into the transmissibility, pathogenesis, entry factors, and immune response of COVID-19. Specifically, Pubmed and Google Scholar databases were searched to identify any relevant articles. References within the included articles were reviewed. Published articles related to search criteria from the onset of the COVID-19 pandemic to March 2022 were included. Results: Viral transmissibility is predominantly affected by the modes of transmission, various mutations on the nucleocapsid protein and endoRNAse, gender, age, and other factors. The pathophysiological mechanism is generally unknown, although the clinical manifestations such as headache, loss of smell and taste, vomiting, diarrhea, multiorgan failure, and dermatological and cardiovascular complications are well documented. The progression of infection depends on the immunopathological response and the innate/adaptive immunity. Conclusion: Our review has summarized the latest knowledge about SARS-CoV2. However, as the pandemic continues to spread across the continents, there is an urgent need for more research on potentially emerging coronaviruses and the development of a universal coronaviruses vaccine to put the pandemic behind us.
Kinetic Alterations in Resurgent Sodium Currents of Mutant Nav1.4 Channel in Two Patients Affected by Paramyotonia Congenita
Authors: Lee Ming-Jen , Lin Pi-Chen , Lin Ming-Hong , Chiou Hsin-Ying Clair , Wang Kai , Huang Chiung-Wei
Published: April 2022

Paramyotonia congenita (PMC) is a rare skeletal muscle disorder characterized by muscle stiffness upon repetitive exercise and cold exposure. PMC was reported to be caused by dominant mutations in the SCN4A gene encoding the α subunit of the Nav1.4 channel. Recently, we identified two missense mutations of the SCN4A gene, p.V781I and p.A1737T, in two PMC families. To evaluate the changes in electrophysiological properties caused by the mutations, both mutant and wild-type (WT) SCN4A genes were expressed in CHO-K1 and HEK-293T cells. Then, whole-cell patch-clamp recording was employed to study the altered gating of mutant channels. The activation curve of transient current showed a hyperpolarizing shift in both mutant Nav1.4 channels as compared to the WT channel, whereas there was a depolarizing shift in the fast inactivation curve. These changes confer to an increase in window current in the mutant channels. Further investigations demonstrated that the mutated channel proteins generate significantly larger resurgent currents as compared to the WT channel and take longer to attain the peak of resurgent current than the WT channel. In conclusion, the current study demonstrates that p.V781I and p.A1737T mutations in the Nav1.4 channel increase both the sustained and the resurgent Na+ current, leading to membrane hyperexcitability with a lower firing threshold, which may influence the clinical phenotype.
Transvenous Lead Extraction in Patients with Cardiac Implantable Device: The Impact of Systemic and Local Infection on Clinical Outcomes—An ESC-EHRA ELECTRa (European Lead Extraction Controlled) Registry Substudy
Authors: Diemberger Igor , Segreti Luca , Rinaldi Christopher A. , Svendsen Jesper Hastrup , Kutarski Andrzej , Younis Arwa , Laroche Cécile , Leclercq Christophe , Małecka Barbara , Mitkowski Przemyslaw , Bongiorni Maria Grazia ,   On behalf of the ELECTRa Investigators
Published: April 2022

Background: Infections of cardiac implantable devices (CIEDI) have poor outcomes despite improvement in lead extraction (TLE) procedures. Methods: To explore the influence of CIEDI on the outcomes of TLE and the differences between patients with systemic (Sy) vs. local (Lo) CIEDI, we performed a sub-analysis of the EORP ELECTRa (European Lead Extraction ConTRolled) Registry. Results: Among 3555 patients enrolled by 73 centers in 19 Countries, the indication for TLE was CIEDI in 1850: 1170 with Lo-CIEDI and 680 with Sy-CIEDI. Patients with CIEDI had a worse in-hospital prognosis in terms of major complications (3.57% vs. 1.71%; p = 0.0007) and mortality (2.27% vs. 0.49%; p < 0.0001). Sy-CIEDI was an independent predictor of in-hospital death (H.R. 2.14; 95%CI 1.06–4.33. p = 0.0345). Patients with Sy-CIEDI more frequently had an initial CIED implant and a higher prevalence of comorbidities, while subjects with Lo-CIEDI had a higher prevalence of previous CIED procedures. Time from signs of CIEDI and TLE was longer for Lo-CIEDI despite a shorter pre-TLE antibiotic treatment. Conclusions: Patients with CIEDI have a worse in-hospital prognosis after TLE, especially for patients with Sy-CIEDI. These results raise the suspicion that in a relevant group of patients CIEDI can be systemic from the beginning without progression from Lo-CIEDI. Future research is needed to characterize this subgroup of patients.
Semen Modulates Cell Proliferation and Differentiation-Related Transcripts in the Pig Peri-Ovulatory Endometrium
Authors: Gardela Jaume , Ruiz-Conca Mateo , Wright Dominic , López-Béjar Manel , Martínez Cristina A. , Rodríguez-Martínez Heriberto , Álvarez-Rodríguez Manuel
Published: April 2022

Uterine homeostasis is maintained after mating by eliminating pathogens, foreign cells, and proteins by a transient inflammation of the uterus. Such inflammation does not occur in the oviductal sperm reservoir (utero-tubal junction, UTJ), colonized by a population of potentially fertile spermatozoa before the inflammatory changes are triggered. Semen entry (spermatozoa and/or seminal plasma) modifies the expression of regulatory genes, including cell proliferation and differentiation-related transcripts. Considering pigs display a fractionated ejaculation, this study aims to determine whether different ejaculate fractions differentially modulate cell proliferation and differentiation-related transcripts in the sow reproductive tract during the peri-ovulatory stage. Using species-specific microarray analyses, the differential expression of 144 cell proliferation and differentiation-related transcripts was studied in specific segments: cervix (Cvx), distal and proximal uterus (DistUt, ProxUt), UTJ, isthmus (Isth), ampulla (Amp), and infundibulum (Inf) of the peri-ovulatory sow reproductive tract in response to semen and/or seminal plasma cervical deposition. Most mRNA expression changes were induced by mating. In addition, while mating upregulates the fibroblast growth factor 1 (FGF1, p-value DistUt = 0.0007; ProxUt = 0.0253) transcript in the endometrium, both its receptor, the fibroblast growth factor receptor 1 (FGFR1, p-value DistUt = 2.14 e−06; ProxUt = 0.0027; UTJ = 0.0458) transcript, and a potentiator of its biological effect, the fibroblast growth factor binding protein 1 (FGFBP1), were downregulated in the endometrium (p-value DistUt = 0.0068; ProxUt = 0.0011) and the UTJ (p-value UTJ = 0.0191). The FGFBP1 was downregulated in the whole oviduct after seminal depositions (p-value Isth = 0.0007; Amp = 0.0007; Inf = 6.87 e−05) and, interestingly, FGFR1 was downregulated in the endometrium in the absence of semen (p-value DistUt = 0.0097; ProxUt = 0.0456). In conclusion, the findings suggest that spermatozoa, seminal components, and the act of mating trigger, besides inflammation, differential mechanisms in the peri-ovulatory female reproductive tract, relevant for tissue repair.
The Impact of Cropland Abandonment of Post-Soviet Countries on the Terrestrial Carbon Cycle Based on Optimizing the Cropland Distribution Map
Authors: Zhou Shengjie , Chen Tiexi , Zeng Ning , Cai Qixiang , Zhao Fang , Han Pengfei , Yan Qingyun
Published: April 2022

Land use and cover changes (LUCC) have a fundamental impact on the terrestrial carbon cycle. The abandonment of cropland as a result of the collapse of the Soviet Union offers a typical case of the conversion from cropland to natural vegetation, which could have a significant effect on the terrestrial carbon cycle. Due to the inaccuracy of LUCC records, the corresponding impact on the terrestrial carbon cycle has not been well quantified. In this study, we estimated the carbon flux using the Vegetation-Global-Atmosphere-Soil (VEGAS) model over the region of Russia, Belarus and Ukraine during 1990–2017. We first optimized the LUCC input data by adjusting the Food and Agriculture Organization (FAO) data by Russian statistical data and redistributing the spatiotemporal input data from the Historical Database of the Global Environment (HYDE) to the original model. Between 1990 and 2017, the area of cropland abandonment was estimated to be 36.82 Mha, compared to 11.67 Mha estimated by FAO. At the same time, the carbon uptake from the atmosphere to the biosphere was 9.23 GtC (vs fixed cropland 8.24 and HYDE 8.25 GtC) during 1990–2017, which means by optimizing the cropland distribution data, the total carbon absorption during the abandonment process increased by 0.99 GtC. Meanwhile, the growth of the vegetation carbon pool was significantly higher than that of the soil carbon pool. Therefore, we further highlight the importance of accurate cropland distribution data in terrestrial carbon cycle simulation.
Identification of Intrinsically Disordered Proteins and Regions in a Non-Model Insect Species Ostrinia nubilalis (Hbn.)
Authors: Avramov Miloš , Schád Éva , Révész Ágnes , Turiák Lilla , Uzelac Iva , Tantos Ágnes , Drahos László , Popović Željko D.
Published: April 2022

Research in previous decades has shown that intrinsically disordered proteins (IDPs) and regions in proteins (IDRs) are as ubiquitous as highly ordered proteins. Despite this, research on IDPs and IDRs still has many gaps left to fill. Here, we present an approach that combines wet lab methods with bioinformatics tools to identify and analyze intrinsically disordered proteins in a non-model insect species that is cold-hardy. Due to their known resilience to the effects of extreme temperatures, these proteins likely play important roles in this insect’s adaptive mechanisms to sub-zero temperatures. The approach involves IDP enrichment by sample heating and double-digestion of proteins, followed by peptide and protein identification. Next, proteins are bioinformatically analyzed for disorder content, presence of long disordered regions, amino acid composition, and processes they are involved in. Finally, IDP detection is validated with an in-house 2D PAGE. In total, 608 unique proteins were identified, with 39 being mostly disordered, 100 partially disordered, 95 nearly ordered, and 374 ordered. One-third contain at least one long disordered segment. Functional information was available for only 90 proteins with intrinsic disorders out of 312 characterized proteins. Around half of the 90 proteins are cytoskeletal elements or involved in translational processes.
Correction: Hashmi et al. Hydrogen Sulphide Treatment Prevents Renal Ischemia-Reperfusion Injury by Inhibiting the Expression of ICAM-1 and NF-kB Concentration in Normotensive and Hypertensive Rats. Biomolecules 2021, 11, 1549
Authors: Hashmi Syed F. , Rathore Hassaan Anwer , Sattar Munavvar A. , Johns Edward J. , Gan Chee-Yuen , Chia Tan Yong , Ahmad Ashfaq
Published: April 2022

In the published publication [...]
The Influence of Heterochronic Non-Myeloablative Bone Marrow Transplantation on the Immune System, Frailty, General Health, and Longevity of Aged Murine Recipients
Authors: Jazbec Katerina , Jež Mojca , Švajger Urban , Smrekar Boštjan , Miceska Simona , Rajčevič Uroš , Justin Mojca , Završnik Janja , Malovrh Tadej , Švara Tanja , Gombač Mitja , Ramšak Živa , Rožman Primož
Published: April 2022

The stem cell theory of aging postulates that stem cells become inefficient at maintaining the original functions of the tissues. We, therefore, hypothesized that transplanting young bone marrow (BM) to old recipients would lead to rejuvenating effects on immunity, followed by improved general health, decreased frailty, and possibly life span extension. We developed a murine model of non-myeloablative heterochronic BM transplantation in which old female BALB/c mice at 14, 16, and 18(19) months of age received altogether 125.1 ± 15.6 million nucleated BM cells from young male donors aged 7–13 weeks. At 21 months, donor chimerism was determined, and the immune system’s innate and adaptive arms were analyzed. Mice were then observed for general health and frailty until spontaneous death, when their lifespan, post-mortem examinations, and histopathological changes were recorded. The results showed that the old mice developed on average 18.7 ± 9.6% donor chimerism in the BM and showed certain improvements in their innate and adaptive arms of the immune system, such as favorable counts of neutrophils in the spleen and BM, central memory Th cells, effector/effector memory Th and Tc cells in the spleen, and B1a and B1b cells in the peritoneal cavity. Borderline enhanced lymphocyte proliferation capacity was also seen. The frailty parameters, pathomorphological results, and life spans did not differ significantly in the transplanted vs. control group of mice. In conclusion, although several favorable effects are obtained in our heterochronic non-myeloablative transplantation model, additional optimization is needed for better rejuvenation effects.
Current Strategies for Real-Time Enzyme Activation
Authors: Wang Fang , Liu Yuchen , Du Chang , Gao Renjun
Published: April 2022

Enzyme activation is a powerful means of achieving biotransformation function, aiming to intensify the reaction processes with a higher yield of product in a short time, and can be exploited for diverse applications. However, conventional activation strategies such as genetic engineering and chemical modification are generally irreversible for enzyme activity, and they also have many limitations, including complex processes and unpredictable results. Recently, near-infrared (NIR), alternating magnetic field (AMF), microwave and ultrasound irradiation, as real-time and precise activation strategies for enzyme analysis, can address many limitations due to their deep penetrability, sustainability, low invasiveness, and sustainability and have been applied in many fields, such as biomedical and industrial applications and chemical synthesis. These spatiotemporal and controllable activation strategies can transfer light, electromagnetic, or ultrasound energy to enzymes, leading to favorable conformational changes and improving the thermal stability, stereoselectivity, and kinetics of enzymes. Furthermore, the different mechanisms of activation strategies have determined the type of applicable enzymes and manipulated protocol designs that either immobilize enzymes on nanomaterials responsive to light or magnetic fields or directly influence enzymatic properties. To employ these effects to finely and efficiently activate enzyme activity, the physicochemical features of nanomaterials and parameters, including the frequency and intensity of activation methods, must be optimized. Therefore, this review offers a comprehensive overview related to emerging technologies for achieving real-time enzyme activation and summarizes their characteristics and advanced applications.
Advances towards Understanding the Mechanism of Action of the Hsp90 Complex
Authors: Prodromou Chrisostomos , Bjorklund Dennis M.
Published: April 2022

Hsp90 (Heat Shock Protein 90) is an ATP (Adenosine triphosphate) molecular chaperone responsible for the activation and maturation of client proteins. The mechanism by which Hsp90 achieves such activation, involving structurally diverse client proteins, has remained enigmatic. However, recent advances using structural techniques, together with advances in biochemical studies, have not only defined the chaperone cycle but have shed light on its mechanism of action. Hsp90 hydrolysis of ATP by each protomer may not be simultaneous and may be dependent on the specific client protein and co-chaperone complex involved. Surprisingly, Hsp90 appears to remodel client proteins, acting as a means by which the structure of the client protein is modified to allow its subsequent refolding to an active state, in the case of kinases, or by making the client protein competent for hormone binding, as in the case of the GR (glucocorticoid receptor). This review looks at selected examples of client proteins, such as CDK4 (cyclin-dependent kinase 4) and GR, which are activated according to the so-called ‘remodelling hypothesis’ for their activation. A detailed description of these activation mechanisms is paramount to understanding how Hsp90-associated diseases develop.
Effects of Reversal of Hypotension on Cerebral Microcirculation and Metabolism in Experimental Sepsis
Authors: Taccone Fabio Silvio , Su Fuhong , He Xinrong , Peluso Lorenzo , Donadello Katia , Scolletta Sabino , De Backer Daniel , Vincent Jean-Louis
Published: April 2022

The effects of reversal of hypotension on the cerebral microcirculation, oxygenation, and metabolism in septic shock remain unclear. In 12 sheep, peritonitis was induced by injection of feces into the abdominal cavity. At the onset of septic shock (mean arterial pressure (MAP) < 65 mmHg, unresponsive to fluid challenge), a norepinephrine infusion was titrated in eight sheep to restore a MAP ≥ 75 mmHg; the other four sheep were kept hypotensive. The microcirculation of the cerebral cortex was evaluated using side-stream dark-field video-microscopy. Brain partial pressure of oxygen (PbtO2) was measured, and cerebral metabolism was assessed using microdialysis. All animals developed septic shock after a median of 15 (14–19) h. When MAP was raised using norepinephrine, the PbtO2 increased significantly (from 41 ± 4 to 55 ± 5 mmHg), and the cerebral lactate/pyruvate ratio decreased (from 47 ± 13 to 28 ± 4) compared with values at shock onset. Changes in the microcirculation were unchanged with restoration of MAP and the glutamate increased further (from 17 ± 11 to 23 ± 16 μM), as it did in the untreated animals. In septic shock, the correction of hypotension with vasopressors may improve cerebral oxygenation but does not reverse the alterations in brain microcirculation or cerebral metabolism.
Extremely Low-Frequency Electromagnetic Fields Increase Cytokines in Human Hair Follicles through Wnt/β-Catenin Signaling
Authors: Choi Ju-Hye , Kim Yu-Mi , Park Hee-Jung , Nam Myeong-Hyun , Seo Young-Kwon
Published: April 2022

Hair loss is a chronic disorder that affects many people; however, a complete treatment has not yet been developed. Therefore, new therapeutic agents for preventing hair loss must be developed, and electromagnetic field (EMF) therapy has been proven to be a promising medical treatment in various fields, including hair loss treatment. This study evaluated the effect of extremely low-frequency electromagnetic field (ELF-EMF) intensity and exposure time by analyzing the expression of cytokines and anagen-related molecules, which influence hair activation and growth, in hair bulb spheroid (HBS) and hair follicle (HF) organ cultures. ELF-EMFs did not induce toxicity in the HBSs, as verified via the lactate dehydrogenase (LDH) assay. Moreover, an ELF-EMF intensity of 5–20 G promoted the expression of ALP, versican, β-catenin, and several cytokines (VEGF, PDGF, FGF-10, and ET-1) in HBSs. Immunohistochemical staining showed that ELF-EMF at an intensity of 5–20 G upregulated ALP and β-catenin and decreased TUNEL staining in HBS. Moreover, HFs exposed to ELF-EMF for 60 min exhibited an increase in hair length and a 1.5-fold increase in IL-4, ICAM-1, ALP, and versican mRNA expression compared to the control. Immunohistochemical staining indicated that 60 min of ELF-EMF can increase the expression of ALP and β-catenin and decreases TUNEL staining in organ cultures. Collectively, our results demonstrated that ELF-EMF exposure at a 10 G intensity for 60 min promoted hair shaft growth in HFs due to the effect of cytokines and adhesion molecules via the Wnt/β-catenin pathway. Therefore, ELF-EMF is a promising treatment for hair loss.
Tandem Repeat Diversity in Two Closely Related Hamster Species—The Chinese Hamster (Cricetulus griseus) and Striped Hamster (Cricetulus barabensis)
Authors: Ivanova Nadezhda G. , Kartavtseva Irina V. , Stefanova Vera N. , Ostromyshenskii Dmitrii I. , Podgornaya Olga I.
Published: April 2022

The Chinese hamster (Cricetulus griseus) and striped hamster (Cricetulus barabensis) are very closely related species with similar karyotypes. The karyotypes differ from each other by one Robertsonian rearrangement and X-chromosome morphology. The level of the tandem repeat (TR) sequences’ evolutional variability is high. The aim of the current work was to trace the TR distribution on the chromosomes of two very closely related species. The striped hamster genome has not yet been sequenced. We classified the Chinese hamster TR in the assemblies available and then compared the mode of the TR distribution in closely related species. Chinese and striped hamsters are separate species due to the relative species specificity of Chinese hamster TR and prominent differences in the TR distribution in both species. The TR variation observed within homologous striped hamster chromosomes is caused by a lack of inbreeding in natural populations. The set of TR tested could be used to examine the CHO lines’ instability that has been observed in heterochromatic regions.
Cell Type-Specific Anti-Adhesion Properties of Peritoneal Cell Treatment with Plasma-Activated Media (PAM)
Authors: Holl Myriam , Rasch Marie-Lena , Becker Lucas , Keller Anna-Lena , Schultze-Rhonhof Laura , Ruoff Felix , Templin Markus , Keller Silke , Neis Felix , Keßler Franziska , Andress Jürgen , Bachmann Cornelia , Krämer Bernhard , Schenke-Layland Katja , Brucker Sara , Marzi Julia , Weiss Martin
Published: April 2022

Postoperative abdominal adhesions are responsible for serious clinical disorders. Administration of plasma-activated media (PAM) to cell type-specific modulated proliferation and protein biosynthesis is a promising therapeutic strategy to prevent pathological cell responses in the context of wound healing disorders. We analyzed PAM as a therapeutic option based on cell type-specific anti-adhesive responses. Primary human peritoneal fibroblasts and mesothelial cells were isolated, characterized and exposed to different PAM dosages. Cell type-specific PAM effects on different cell components were identified by contact- and marker-independent Raman imaging, followed by thorough validation by specific molecular biological methods. The investigation revealed cell type-specific molecular responses after PAM treatment, including significant cell growth retardation in peritoneal fibroblasts due to transient DNA damage, cell cycle arrest and apoptosis. We identified a therapeutic dose window wherein specifically pro-adhesive peritoneal fibroblasts were targeted, whereas peritoneal mesothelial cells retained their anti-adhesive potential of epithelial wound closure. Finally, we demonstrate that PAM treatment of peritoneal fibroblasts reduced the expression and secretion of pro-adhesive cytokines and extracellular matrix proteins. Altogether, we provide insights into biochemical PAM mechanisms which lead to cell type-specific pro-therapeutic cell responses. This may open the door for the prevention of pro-adhesive clinical disorders.