bluuebunny/crossref_metadata_2025 huggingface dataset:
Benchmarking asset pricing models in emerging markets: evidence from Borsa Istanbul
Authors: Gökgöz Fazıl , Seyhan Canan
Published: March 2024

Purpose

Investors who can transfer their savings to investments in a well-regulated market benefit not only themselves but also economic development. Hence, it is crucial for fund owners to evaluate their stock market investment decisions. The goal of the study is to understand which model determines the asset returns most efficiently. In this regard, the validity of single and multi-index asset pricing models (capital asset pricing model-CAPM and Fama–French models) has been examined in the Turkish Stock Exchange for 2009–2020, with the quantile regression (QR) approach.

Design/methodology/approach

On 18 portfolios comprised of quoted stocks in the Istanbul Stock Exchange 100 (ISE-100/BIST-100), we test the CAPM, the Fama and French three factor model (FF3) and the Fama and French five factor model (FF5). Empirical analyses have been carried out via QR approach regressing the portfolios' average weekly excess returns on risk premium/market factor (Rm-Rf), firm size, book value/market value (B/M), profitability and investments factors. QR estimation has been employed since QR is more effective and provides a better definition of the distribution’s tails.

Findings

Our empirical findings have revealed that the average excess weekly returns can be explained more strongly via CAPM. Moreover, Fama and French models are expected to give more reliable result with more data, whereas the market premium would give robust results for the Turkish Capital Market.

Practical implications

Individuals investing in financial assets must find the price model that best fits the market. The return can be approximated in the most appropriate manner using the right variables.

Originality/value

The study differs from other research by comparing the asset pricing models via examining the assets' weekly returns with QR in the Istanbul Stock Exchange 100 (ISE-100).
Implementation of an adapted Handwriting Without Tears® programme for children with developmental disabilities in a special education setting
Authors: Goyen Emily , Grindle Corinna , Totsika Vaso , Sapiets Suzi Jayne
Published: March 2024

Purpose

Children with developmental disabilities (e.g. learning disability and autism) often struggle with handwriting skills. This study aims to implement an adapted handwriting programme for children with developmental disabilities to improve their handwriting skills.

Design/methodology/approach

Six children with developmental disabilities aged 9–15 years received an adapted Handwriting Without Tears® (HWT) programme in small groups over eight weeks. The programme was delivered by typical teaching staff (i.e. paraprofessionals) at a special education school following a brief training session and with ongoing supervision. A range of measures assessed the children’s handwriting and related skills. Social validity interviews were conducted with school staff following the intervention to evaluate the programme’s acceptability.

Findings

Typical teaching staff implemented the handwriting programme with 92.3% average fidelity and delivered a minimum of three sessions per week. Social validity interviews demonstrated the acceptability of the intervention to school staff. After eight weeks of intervention, all children improved their handwriting on various assessments. Improvements were only partially maintained at follow-up.

Originality/value

This study supports the feasibility of using an adapted HWT programme to teach handwriting to children with developmental disabilities in special education settings. Typical teaching staff can be trained to support the delivery of the programme to children in small groups.
‘Safe as Houses’: Recalibrating Homeownership in Australian Retirement Planning
Authors: Chen Jane , Tran Long , Sinclair Sarah , Boymal Jonathan
Published: March 2024

Australia's longstanding culture of homeownership, symbolising stability and achievement, also impacts positive ageing outcomes across multiple domains. Recently, demographic and economic factors, such as housing affordability issues, insufficient income and barriers to the housing market, pose challenges to homeownership, with implications for retirement planning as the use of home equity to support ageing evolves. Secure housing and financial security are critical for successful ageing, necessitating flexible solutions and innovative financing models like fractional ownership. We raise awareness of the changing dynamics of retirement planning and accentuate the increasing significance of innovative housing solutions in supporting health during retirement.
Detection and quantification of biogenic amines in cephalopod using dansyl chloride pre‐column derivatization‐HPLC and their production
Authors: Wang Kang , Pan Chuanyan , Yang Qiong , Ruan Qiufeng , Chen Weijie , Lv Min , Yang Lifang , Zou Zhiming , Ma Huawei
Published: March 2024

The accurate detection of biogenic amines (BAs) is an important means of ensuring the quality and safety of cephalopod seafood products. In this study, the pre‐column derivatization of high‐performance liquid chromatography (HPLC) was optimized using dansyl chloride (Dns‐Cl) to detect BAs in octopus, cuttlefish, and squid. The reasons for the formation of BAs were investigated by assessing their decarboxylase activity and the rates of decomposition. The findings demonstrated that using Dns‐Cl to optimize pre‐column derivatization enabled the separation of nine different BAs. The detection limits ranged from 0.07 to 0.25 mg/L, and the results exhibited a high level of linearity (R2 ≥ 0.997). The decarboxylase activity and biodegradation rate positively correlated with the formation of BAs at temperatures below 0°C. Notably, the decarboxylase activity of octopus, cuttlefish, and squid exhibited a significant increase with prolonged storage time, and formyltransferase and carbamate kinase may be the key decarboxylase in cephalopod products. These findings serve as a valuable reference for further investigations into the mechanisms behind BAs production and the development of control technologies for BAs in cephalopod products. This study has successfully demonstrated the effectiveness of the Dns‐Cl pre‐column derivatization‐HPLC method in accurately and efficiently detecting BAs in octopus, cuttlefish, and squid. Moreover, it highlights the influence of decarboxylase content and biodegradation rate on the formation of BAs. Importantly, this method can serve as a reference for detecting BAs in various seafood products.
Enhancing citric acid tolerance of Acetobacter tropicalis using chemical and physical mutagenesis and adaptive evolution to improve the quality of lemon fruit vinegar
Authors: Yang Shaojie , Li Kang , Liu Hua , Lu Jian , Yang Hua , Wu Dianhui
Published: March 2024

The high concentration of citric acid in lemons limits the production of lemon fruit vinegar because it inhibits the metabolism of acetic acid bacteria and reduces the utilization of raw materials. This study aimed to enhance the citric acid tolerance of Acetobacter tropicalis by using complex mutagenesis and adaptive laboratory evolution (ALE) and improving the quality of lemon fruit vinegar. After mutagenesis and ALE, A. tropicalis JY‐135 grew well under 40 g/L citric acid, and it showed high physiological activity and excellent fermentation performance under high concentrations of citric acid. The survival rate and ATP content of JY‐135 were 15.27 and 9.30 times higher than that of the original strain J‐2736. In the fermentation of lemon fruit vinegar, the acid production and the number of aroma‐active compounds were 1.61‐fold and 2.17‐fold than J‐2736. In addition, we found that citric acid tolerance of JY‐135 is related to the respiratory electron‐transport chain and the tricarboxylic acid (TCA) cycle. This work is of great significance for the production of high‐quality lemon fruit vinegar and the enrichment of seed resources of acetic acid bacteria.
Determination of the process effect on cocoa butter crystallization by rheometer: Kinetic modeling by Gompertz equation
Authors: Bölük Esra , Akdeniz Esra , Gunes Recep , Palabiyik Ibrahim , Konar Nevzat , Toker Omer Said
Published: March 2024

In this study, the effects of temperature (22, 24, 26, 28, and 30°C) and strain (0.1%, 1%, and 5%) on cocoa butter (CB) crystallization were investigated by oscillatory test, and the four‐parameter Gompertz model was used to interpret the effect of parameters on pre‐crystallization, nucleation, and crystal growth stages of CB. Lag time and growth rate were calculated using the Gompertz model using time‐dependent storage modulus (G′) data. According to the results, CB crystallization at 26°C with a 1% strain value had the highest growth rate value, the shortest lag time, and the formation of βv polymorph type. Followingly, polymorphic types of the CB crystals were determined based on the melting points of polymorphs via the temperature ramp step, and the results obtained were correlated with a polarized light microscope. In conclusion, using a rheometer in both the observation of the pre‐crystallization process and the determination of polymorph types is very important for research and development studies in the chocolate industry for process and formulation optimization.

Practical Application

This study demonstrates the feasibility of a novel approach for investigating crystallization and oscillatory shear of CB using a rheometer, both for observing crystallization kinetics and determining polymorph type, accompanied by the Gompertz equation to model the crystallization kinetics. According to the results, the effect of process parameters (temperature and shear) on the crystallization behavior of CB can be observed by rheometer, which can provide a detailed perspective for chocolate manufacturers and researchers in research and development studies.
Banking bad? A global field experiment on risk, reward, and regulation
Authors: Findley Michael G. , Nielson Daniel L. , Sharman J. C.
Published: March 2024

Are banks sensitive to risk and reward in following global corporate transparency rules? Using a worldwide field experiment, this study evaluates competing predictions from expected utility, behavioralist, and institutionalist accounts. We incorporated a dozen companies around the world to make over 15,000 email solicitations asking for corporate accounts from 5000 of the world's internationally connected banks. Treatments randomize the risk profiles of different companies—by their countries’ association with corruption, terrorism, and tax evasion—and vary rewards by stating differing amounts of business revenues. The outcomes are the rates at which banks offer accounts and comply with rules on customer identification. The results suggest that banks are moderately responsive to risk—though not reward—but the magnitude of the effects is small, providing mixed evidence for conventional models and suggestive support for institutionalist accounts.
Historical lime‐based flooring mortars from the Church of Santa Maria de Alcobaça monastery (12th century), Portugal: A multi‐analytical approach
Authors: Carvalho Fernanda , Nunes Ana , Pagará Ana , Costeira Isabel , da Silva Teresa Pereira , Lima Maria Margarida Rolim Augusto , Veiga João Pedro
Published: March 2024

The Monastery of Alcobaça houses in reserve the ceramic tiles that adorned the floor of the church's apse. These tiles were removed during rehabilitation works and many preserve part of their original fixing mortars. A comprehensive analysis of 21 samples was conducted using a multi‐analytical approach (X‐ray fluorescence, X‐ray diffraction, scanning electron microscopy–energy‐dispersive X‐ray spectroscopy, μ‐Raman spectroscopy, thermogravimetry–differential thermal analysis, optical microscopy, and colorimetry). Results suggest compositional variations in the samples from the back and sides of the ceramic tiles; however, the mineralogy and general characteristics of the aggregates remained consistent between the samples and are coherent with the local geology, suggesting a shared historical origin.
The unheard echoes of equality: perceptions of wealth inequality in the Czech Republic
Authors: Červenka Filip , Maialeh Robin
Published: March 2024

This study examines various attitudes to wealth inequality in Czechia and the broader CEE region and provides valuable insights into global research on inequality perceptions. By focusing on this, to date, underexplored region, our research contributes to the current understanding of wealth inequality at the global scale. The findings revealed remarkable similarities between Czech and US respondents in terms of their perceptions and preferences. On average, participants estimated that the top 20% of the population in the Czech Republic holds approximately 48% of total wealth, while expressing a preference for a lower wealth share of around 34% in the ideal scenario. These results are in alignment with previous research that highlighted a prevalent lack of awareness of actual levels of wealth inequality combined with a consistent preference for the reduction of inequality levels. By elucidating these patterns, this study contributes to the growing body of literature in the field.
Carcass characteristics, meat quality, and nutritional profiles of Mithun (Bos frontalis) meat reared under a semi‐intensive system
Authors: Lalchamliani  , Chauhan Geeta , Ramesh Vikram , Shivanagowda Girish P. , Khate Kobu , Karabasanavar Nagappa S. , Mendiratta Sanjod K. , Haque Nazrul , Mitra Abhijit
Published: March 2024

The present study aimed to determine carcass characteristics, meat quality, nutrient profiles, and sensory characteristics of Mithun meat. Sixteen Mithun were selected and divided into four groups, MM‐4 (male; n = 4; <4 years of age), MM‐47 (male; n = 4; 4–7 years of age), MF‐4 (female; n = 4; <4 years of age), MF‐47 (female; n = 4; 4–7 years of age). Carcass characteristics showed that adult males (MM‐47) have significantly higher (P < 0.05) live weight, carcass weight, and meat‐to‐bone ratio. Fat (%) was significantly higher (P < 0.05), and deboned meat (%) was lower in MF‐4 and MF‐47, while marginal differences were observed in bone (%), dressing percentage, and offal yield between groups. Visible marbling increased with age and varied from “slight” to “small” in all groups. Nutrient profiling revealed a significantly higher (P < 0.05) fat percentage and cholesterol in MF‐4 and MF‐47. Fatty acid profile, amino acid profile, water‐soluble vitamins, and minerals content did not differ between groups. However, lysine and leucine (essential amino acids) and glutamic acid and aspartic acid (nonessential amino acids) were most abundant. Effect of age was significant (P < 0.05) on juiciness, tenderness, and connective tissue residue scores. In conclusion, results indicate mithun meat is nutrient‐rich regardless of the animal's age or sex.
The effects of parental presence on amygdala and mPFC activation during fear conditioning: An exploratory study
Authors: Abramson Lior , Callaghan Bridget L. , Silvers Jennifer A. , Choy Tricia , VanTieghem Michelle , Vannucci Anna , Fields Andrea , Tottenham Nim
Published: March 2024

Learning safe versus dangerous cues is crucial for survival. During development, parents can influence fear learning by buffering their children's stress response and increasing exploration of potentially aversive stimuli. Rodent findings suggest that these behavioral effects are mediated through parental presence modulation of the amygdala and medial prefrontal cortex (mPFC). Here, we investigated whether similar parental modulation of amygdala and mPFC during fear learning occurs in humans. Using a within‐subjects design, behavioral (final N = 48, 6–17 years, mean = 11.61, SD = 2.84, 60% females/40% males) and neuroimaging data (final N = 39, 6–17 years, mean = 12.03, SD = 2.98, 59% females/41% males) were acquired during a classical fear conditioning task, which included a CS+ followed by an aversive noise (US; 75% reinforcement rate) and a CS−. Conditioning occurred once in physical contact with the participant's parent and once alone (order counterbalanced). Region of interest analyses examined the unconditioned stress response by BOLD activation to the US (vs. implicit baseline) and learning by activation to the CS+ (vs. CS−). Results showed that during US presentation, parental presence reduced the centromedial amygdala activity, suggesting buffering of the unconditioned stress response. In response to learned stimuli, parental presence reduced mPFC activity to the CS+ (relative to the CS−), although this result did not survive multiple comparisons’ correction. These preliminary findings indicate that parents modulate amygdala and mPFC activity during exposure to unconditioned and conditioned fear stimuli, potentially providing insight into the neural mechanisms by which parents act as a social buffer during fear learning.

Research Highlights

This study used a within‐participant experimental design to investigate how parental presence (vs. absence) affects youth's neural responses in a classical fear conditioning task.

Parental presence reduced the youth's centromedial amygdala activation to the unconditioned stimulus (US), suggesting parental buffering of the neural unconditioned response (UR).

Parental presence reduced the youth's mPFC activation to a conditioned threat cue (CS+) compared to a safety cue (CS−), suggesting possible parental modulation of fear learning.
Bird‐mediated endozoochory as a potential dispersal mechanism of bony fishes
Authors: Lovas‐Kiss Ádám , Antal László , Mozsár Attila , Nyeste Krisztián , Somogyi Dóra , Kiss Balázs , Tóth Richárd , Tóth Flórián , Lilla Fazekas Dorottya , Vitál Zoltán , Halasi‐Kovács Béla , Tóth Pál , Szabó Nándor , Löki Viktor , Vincze Orsolya , András Lukács Balázs
Published: March 2024

The dispersal of fish into distant and isolated habitats remains a topic of continuous discussion in the field of fish biogeography. This is particularly relevant due to the perceived limitation of fish movement to what is known as active dispersal. Fish migration is often confined to interconnected water bodies, underscoring the significance of dispersal for fish inhabiting isolated aquatic habitats. However, empirical evidence for a natural (i.e. not human‐mediated) mechanism has been limited. Here we explore and provide evidence for waterbird‐mediated endozoochory as a possible dispersal mechanism in various fish species and families. We force‐fed mallards Anas plathyrynchos with fertilised eggs of nine bony fish species, covering nine taxonomic families. We recovered viable embryos of five fish taxa in the faeces of mallard, proving the ability of fish eggs to survive the passing of the digestive system of waterbirds. Moreover, the recovered eggs successfully hatched into larvae in two fish species. Taking into the flight speed and numerosity of mallards, as well as the high abundance of fish eggs, our results highlight endozoochory of fish eggs by waterbirds as a possible significant, although likely rare natural dispersal mechanism that can occur across more species than previously known in freshwater fish.
Influence of victims' diet on the life‐history traits in cannibalistic Menochilus sexmaculatus
Authors: Yadav Tripti , Singh Gaurvanvita , Omkar  , Mishra Geetanjali
Published: March 2024

Multitrophic interactions are common in nature and play a major role in modulating life‐history traits in predatory insects. The sequestration of chemicals and the transfer of nutrients from one trophic level to a higher trophic level have been reported previously. However, the effect of cannibalism, which is prevalent in the food chain, has not been explored yet. In this study, we hypothesised that the varying nutritional condition of the victim would significantly influence the life‐history traits of adult Menochilus sexmaculatus Fabricius (Coleoptera: Coccinellidae). To test this hypothesis, we provided fourth‐instar individuals with first‐instar victims of varying nutritional quality for cannibalism. To create victims of varying nutritional quality, first‐instar victims were fed on high‐quality aphids [Aphis craccivora Koch (Hemiptera: Aphididae)], low‐quality aphids (Aphis nerii Boyer de Fonscolombe) and conspecific eggs. The cannibalistic groups were tested against the control group, in which the fourth instars were provided with their natural prey, A. craccivora. Our results revealed a significant difference in the consumption of prey by the fourth instars between the diet groups, with the control group consuming the most prey. The developmental duration of fourth instars was not significantly influenced by the victims' diet or the amount of victims cannibalised. Furthermore, neither the victims' diet nor the amount of victims cannibalised affected the mating (time to commence mating and copulation duration) and reproductive parameters (egg viability and fecundity) in M. sexmaculatus. Cannibalism here might have compensated for the low‐quality victims, resulting in insignificant effects on the life‐history traits of M. sexmaculatus. Otherwise, given the short duration of the first instars and their potentially limited ability to sequester significant amounts of plant allelochemicals, it is possible that their diet did not affect the development, mating and reproductive attributes of the cannibals. Thus, the use of higher instars as victims may yield substantially different outcomes.
Consumption and preference rates of the various larval stages of Allograpta exotica as biological control agents of Myzus persicae and Frankliniella gemina
Authors: Maza Noelia , Páez‐Jerez Paula Gabriela , Bressán Emilse , Funes Claudia Fernanda , Kirschbaum Daniel Santiago
Published: March 2024

Fruit and vegetable crops are affected by numerous arthropods, including aphids and thrips. Hoverfly adults are important pollinators, and their larvae are mainly aphid predators. Molecular studies on the stomach contents of larvae allowed to associate hoverflies with the consumption of other soft‐bodied arthropods, including thrips, with few records found in Neotropical hoverfly species. Allograpta exotica (Wiedemann) (Diptera: Syrphidae), a species widely distributed in the Americas, from the USA to Argentinian Patagonia, has been associated with numerous crops mainly affected by aphids, and to a lesser extent with whiteflies. The objective of this work was to assess the consumption and preference rates of various instars of A. exotica as biocontrol agents for two major insect pests, Myzus persicae (Sulzer) (Hemiptera: Aphididae) and Frankliniella gemina (Bagnall) (Thysanoptera: Thripidae), under controlled laboratory conditions. Additionally, we morphologically characterized the three larval stages of A. exotica. We determined that all three instars of A. exotica consume both M. persicae and F. gemina, and that they kill more thrips than aphids. Still, A. exotica preferred M. persicae over F. gemina, even when the relative aphid availability decreased compared to F. gemina, although consumption of both prey types remained high. Interestingly, A. exotica second instars consumed four times more thrips than aphids. This study explored the potential use of one of the most common hoverfly species in fruit and vegetable crops as a biocontrol agent of aphids and thrips. Further laboratory and field studies should explore more deeply the potential of this Neotropical beneficial dipteran.
The natural history of luck: A synthesis study of structured population models
Authors: Hernández Christina M. , Ellner Stephen P. , Snyder Robin E. , Hooker Giles
Published: March 2024

Chance pervades life. In turn, life histories are described by probabilities (e.g. survival and breeding) and averages across individuals (e.g. mean growth rate and age at maturity). In this study, we explored patterns of luck in lifetime outcomes by analysing structured population models for a wide array of plant and animal species. We calculated four response variables: variance and skewness in both lifespan and lifetime reproductive output (LRO), and partitioned them into contributions from different forms of luck. We examined relationships among response variables and a variety of life history traits. We found that variance in lifespan and variance in LRO were positively correlated across taxa, but that variance and skewness were negatively correlated for both lifespan and LRO. The most important life history trait was longevity, which shaped variance and skew in LRO through its effects on variance in lifespan. We found that luck in survival, growth, and fecundity all contributed to variance in LRO, but skew in LRO was overwhelmingly due to survival luck. Rapidly growing populations have larger variances in LRO and lifespan than shrinking populations. Our results indicate that luck‐induced genetic drift may be most severe in recovering populations of species with long mature lifespan and high iteroparity.
Therapeutic choices and disease activity after 2 years of treatment with cladribine: An Italian multicenter study (CladStop)
Authors: Schiavetti Irene , Signori Alessio , Albanese Angela , Frau Jessica , Cocco Eleonora , Lorefice Lorena , di Lemme Sonia , Fantozzi Roberta , Centonze Diego , Landi Doriana , Marfia Girolama , Signoriello Elisabetta , Lus Giacomo , Zecca Chiara , Gobbi Claudio , Iodice Rosa , Malimpensa Leonardo , Cordioli Cinzia , Ferraro Diana , Ruscica Francesca , Pasquali Livia , Repice Anna , Immovilli Paolo , Ferrò Maria Teresa , Bonavita Simona , Di Filippo Massimiliano , Abbadessa Gianmarco , Govone Flora , Sormani Maria Pia ,   CladStop study group
Published: March 2024

Background and purpose

Cladribine tablets, a purine analogue antimetabolite, offer a unique treatment regimen, involving short courses at the start of the first and second year, with no further treatment needed in years 3 and 4. However, comprehensive evidence regarding patient outcomes beyond the initial 24 months of cladribine treatment is limited.

Methods

This retrospective, multicenter study enrolled 204 patients with multiple sclerosis who had completed the 2‐year course of cladribine treatment. The primary outcomes were therapeutic choices and clinical disease activity assessed by annualized relapse rate after the 2‐year treatment course.

Results

A total of 204 patients were enrolled; most patients (75.4%) did not initiate new treatments in the 12 months postcladribine. The study found a significant reduction in annualized relapse rate at the 12‐month follow‐up after cladribine completion compared to the year prior to starting therapy (0.07 ± 0.25 vs. 0.82 ± 0.80, p < 0.001). Furthermore, patients with relapses during cladribine treatment were more likely to start new therapies, whereas older patients were less likely. The safety profile of cladribine was favorable, with lymphopenia being the primary registered adverse event.

Conclusions

This study provides insights into therapeutic choices and disease activity following cladribine treatment. It highlights cladribine's effectiveness in reducing relapse rates and disability progression, reaffirming its favorable safety profile. Real‐world data, aligned with previous reports, draw attention to ocrelizumab and natalizumab as common choices after cladribine. However, larger, prospective studies for validation and a more comprehensive understanding of cladribine's long‐term impact are necessary.
A co‐created multimethod evaluation of recovery education in Ireland
Authors: O'Brien Ann , Murphy Louise , Hunt Amanda , Dwyer David , Hunter Andrew
Published: March 2024

Background

This paper aims to explore the impact of recovery education on recovery knowledge, attitudes and the quality of life of students undertaking recovery education, contributing to the evidence base in relation to the impact of recovery education. It also explores the experiences of all stakeholders involved in the co‐facilitation, delivery and participation in recovery education.

Setting and Participants

This study evaluates the experiences of stakeholders involved in the co‐facilitation, delivery and participation in recovery education across four recovery colleges in Ireland. Participants included students undertaking recovery education, peer educators, education facilitators, recovery college coordinators and practitioner/service providers.

Discussion

Findings from the quantitative survey when compared with extant literature suggest that students had a good understanding of recovery education. The social aspect of empowerment for growth and wellbeing was identified through themes relating to co‐production and facilitating student learning. Support for equitable access to recovery education, including co‐production for both the public and staff, was identified as a challenge for the future.

Conclusion

The findings from both the qualitative and quantitative components of the study show the positive impact of recovery education on stakeholders while acknowledging the need for ongoing support for people working in recovery education and the development of services. In particular, there was a high level of recovery knowledge found in students undertaking recovery education.

Patient or Public Contribution

This study utilised a co‐created study design. From inception a steering group comprising stakeholders (peer educators, recovery education facilitators including past recovery college students and nonpeer staff involved in the co‐production of recovery education) directed the conduct of the evaluation. This steering group participated in an iterative process of information sharing, suggestions for evaluation process and language.
Effects of a shared decision‐making implementation programme on patient‐centred communication in oncology—Secondary analysis of a randomised controlled trial
Authors: Lindig Anja , Mannagottera Lotta , Hahlweg Pola , Sigl Hannah , Klimesch Anne , Zeh Stefan , Kriston Levente , Scholl Isabelle
Published: March 2024

Background

There is a need for better implementation of patient‐centred (PC) communication and shared decision‐making (SDM) in routine cancer care.

Objective

The aim of this study was to assess whether a programme to implement SDM in oncology had effects on PC communication in clinical encounters.

Design

This study constitutes a secondary analysis of data derived from an implementation trial applying a stepped wedge design that, among other strategies, incorporated training and coaching to enhance the PC communication skills of physicians.

Setting and Participants

We analysed audio recordings of clinical encounters collected in three departments of a comprehensive cancer centre in Germany before and after rolling out the implementation programme.

Main Variables Studied

We assessed the PC communication skills of physicians.

Main Outcome Measures

Each recording was rated by two researchers using the German version of the Four Habits Coding Scheme (4HCS), an observer‐based measure of PC communication. Interrater reliability of the outcome measure was acceptable but moderate. Demographic data of patients participating in audio recordings were analysed.

Methods

Data were analysed using descriptive statistics and linear mixed‐effects models.

Results

In total, 146 encounters, 74 before and 72 after implementation, were evaluated. The mean age of patients was 57.1 years (SD = 13.8), 70.3% were female, the largest portion of patients had medium formal education (32.4%) and were (self‐) employed (37.8%). No statistically significant effect of the implementation programme on the physicians' PC communication skills was found.

Discussion

The results indicate that the investigated programme to implement SDM in oncology, including training and coaching, had no effects on PC communication in clinical encounters. These results are in contrast to other studies that report the effects of specific training or coaching on PC communication. Reasons for the lack of effect include the short duration of our training compared to other studies, limited reliability and moderate interrater reliability of the 4HCS scale, limited reach of the intervention programme as well as the inclusion of physicians regardless of their exposure to the interventions.

Conclusion

Further research is needed to develop implementation strategies that improve physicians' PC communication skills.

Patient Contribution

Data on patients and clinical encounters with patients and physicians were analysed. There was no other patient or public involvement.
Low molecular weight peptides in tauco, a fermented soy product, associated to umami taste through peptidomics‐sensomics approach
Authors: Herlina Vika Tresnadiana , Lioe Hanifah Nuryani , Kusumaningrum Harsi Dewantari , Adawiyah Dede Robiatul
Published: March 2024

Umami peptides from ultrafiltration fractions of tauco have never been revealed yet. This study's objective was to determine umami peptides by peptidomics‐sensomics approach followed by molecular docking on the ultrafiltration fractions of tauco with MWs <3 kDa from different regions of Indonesia. Sensory evaluation by taste dilution analysis to determine umami intensity and peptide identification by HR‐LC–MS/MS were conducted. Seventeen peptides were revealed to be associated with the intense umami taste of tauco fractions, having molecular weights of 1106–1760 Da. The molecular mechanism and binding energy affinity of the peptides to umami receptor T1R1/T1R3 heterodimer through molecular docking confirmed the result. Peptides IPVNKPGRF, DVFRAIPSEV, and DVTDVTGDRGVTVT are 3 of the 17 identified peptides which have the lowest binding energy to umami receptor T1R1/T1R3 at −9.4, −9.2, and −9.1 kcal mol−1, respectively. In addition, the molecular docking results indicated that His145, Asn68, Val277, and Ser67 of taste receptor type 1 member 3, T1R3 subunit, play critical roles in the binding with identified umami peptides by forming hydrogen bonds and hydrophobic interaction.
Widely targeted metabolomics reveals the species‐specific, matureness‐specific and post‐harvest‐specific discriminations in the chemical profiles of Vietnamese endemic golden camellias
Authors: Nguyen Phuong Nhi , Do Thi Yen , Do Thi Nhung , Gontier Eric , Le Nguyen Ha Trang , Le Thi Van Anh , Mai Nga T.P. , Sato Muneo , Hirai Masami Yokota , Nguyen Thi Kieu Oanh
Published: March 2024

Vietnam is one of the countries with the highest diversity of golden camellias in the world, with more than forty endemic species. However, studies on Vietnamese golden camellias need to be expanded to fill a knowledge gap on these species' chemical profiles and pharmacological properties. This qualitative study focuses on six golden camellias: four endemic species from Tam Dao district, Vinh Phuc province, including Camellia hakodae, Camellia phanii, Camellia tamdaoensis, Camellia tienii and two species commonly cultivated in Quang Ninh province including Camellia petelotii and Camellia euphlebia. A mutual profile of 131 metabolites was obtained by using widely targeted metabolomics analysis using ultra‐performance liquid chromatography coupled with tandem mass spectrometry. Comparative metabolomics was performed among leaf extracts of six golden teas, between young and mature leaves and between freeze‐drying and oven‐drying leaves to discriminate these species, and determine the effect of developmental stage and post‐harvesting method on the plant metabolism. The PLS‐DA model successfully illustrated a clear separation of the six species and suggested apigenin C‐glycoside derivatives as chemical markers for the diversity. Young and mature leaves of all six species also produced distinguished metabolomes, differentiated by mostly amino acid derivatives. The multivariate analysis also indicated the conversion of some flavonoids and amino acids induced by heat in the drying process. These results offer metabolite markers for quality control of Vietnamese golden camellias and recommendations for their usage and preparation.
Trained immunity: General and emerging concepts
Authors: Vuscan Patricia , Kischkel Brenda , Joosten Leo A. B. , Netea Mihai G.
Published: March 2024

Over the past decade, compelling evidence has unveiled previously overlooked adaptive characteristics of innate immune cells. Beyond their traditional role in providing short, non‐specific protection against pathogens, innate immune cells can acquire antigen‐agnostic memory, exhibiting increased responsiveness to secondary stimulation. This long‐term de‐facto innate immune memory, also termed trained immunity, is mediated through extensive metabolic rewiring and epigenetic modifications. While the upregulation of trained immunity proves advantageous in countering immune paralysis, its overactivation contributes to the pathogenesis of autoinflammatory and autoimmune disorders. In this review, we present the latest advancements in the field of innate immune memory followed by a description of the fundamental mechanisms underpinning trained immunity generation and different cell types that mediate it. Furthermore, we explore its implications for various diseases and examine current limitations and its potential therapeutic targeting in immune‐related disorders.
It was better before COVID: The impact of the social relief of distress grant on the lives and livelihoods of South African day labourers in Tshwane
Authors: Venter Lodewalt , Blaauw Derick , Claassen Carike
Published: March 2024

Persistent high unemployment in South Africa rendered many in the informal economy structurally vulnerable to shocks such as the COVID‐19 pandemic. The social relief of distress (SRD) grant was a government attempt to alleviate the pandemic's fallout. Given the lack of scholarly information on the impact and reach of the SRD grant within the informal economy, particularly on day labourers, this study investigates the impact of the SRD grant on the lives and livelihoods of South African day labourers in Tshwane, South Africa. The study adopted a qualitative research design, utilising in depth interviews, transcription, inductive qualitative coding and thematic analysis. Many day labourers could not access the SRD grant due to, for example the loss or absence of identity documents, and/or lack of Internet access and information on the application process. For those who received it, some pressure relating to the cost of transport and basic needs was alleviated. Occasionally, day labourers use it to supplement other funds to acquire specialised tools. Importantly, the grant was not a disincentive to continue their work‐seeking activities. The findings question the effectiveness of policy responses to economic crises within the informal sector.
Shared quantitative trait loci underlying root biomass and phenology in wheat (Triticum aestivum L.)
Authors: Shaltouki‐Rizi Maliheh , Smith Nathan Evan , Brown‐Guedira Gina , Mohammadi Mohsen
Published: March 2024

In this study, we investigated the genetic mapping of root biomass and root/shoot ratio. We utilized a large (n = 345) bi‐parental recombinant inbred line (RIL) population from the ‘Penny’ × ‘Yecora‐Rojo’ cross to investigate the partitioning of biomass above‐ and belowground and to identify the quantitative trait loci (QTL) that influence root biomass and root/shoot ratio. Genotyping of 345 RILs by using genotyping by sequencing produced 2918 single‐nucleotide polymorphism markers by which a genome‐wide map of 3507 cM was constructed. Phenotyping was conducted in an augmented design with large pots in controlled environment. We identified two significant QTL regions, QRt.peye‐5A and QRt.peye‐5B, which control root biomass and the root/shoot ratio. QRt.peye‐5A, marking a 3.15 Mbp region on chromosome 5A, explained 11% of variations in root biomass and 9.5% of variations in root/shoot ratio, with the narrow region harbouring 28 genes. QRt.peye‐5B, marking a 12.2 Mbp region on chromosome 5B, explained 7% of variations in root/shoot ratio and harbours 104 genes. The root/shoot ratio enhancing alleles at QRt.peye‐5A and QRt.peye‐5B come from ‘Penny’ and ‘Yecora‐Rojo’ respectively. These QTL regions contains genes such as the two MADS box transcription factors on the 5A QTL that are candidate genes for Vrn1 locus, and other genes previously postulated for root traits such as a COBRA‐like COBL2 and landmark hormonal responses genes such as IAA16, IAA4 and BRI1, DREB2A‐INTERACTING PROTEIN2 (DRIP2) and bHLH92 which has a role in amelioration of stress conditions.
Ecophysiological mechanisms underlying the positive relationship between seed protein concentration and yield in soybean under field heat and drought stress
Authors: Ergo Verónica V. , Veas Rodolfo E. , Vega Claudia R. C. , Lascano Ramiro , Carrera Constanza S.
Published: March 2024

A positive relationship between protein concentration and yield has been documented in different combinations of genotype and environment, often under potential conditions. However, the ecophysiological bases underlying this positive relationship under heat stress (HS) and drought stress (DS) during seed filling are still lacking. Our objective was to evaluate the relationship between seed protein content and concentration with yield in field experiments exposed to HS, DS and HS × DS interaction during the seed filling. Two field experiments were conducted and assimilates accumulation, remobilization and redistribution patterns were analysed in high and low seed protein soybean genotypes. The crop was exposed to four treatments: control (ambient temperature and soil water content near field capacity), HS (episodes above 32°C, 6 h d−1) during 15 days, DS (soil water content ≤25% of field capacity) during the whole seed filling and HS × DS. Significant and positive relationships between seed protein content and concentration with yield were observed across treatments and genotypes. Under DS and HS × DS, assimilates available during the seed filling decreased, and assimilates remobilization and partition to seeds were limited, responses significantly associated with seed protein content and concentration, and yield reductions. Furthermore, we demonstrated here that the high leaf N content at the beginning of seed filling, the short early reproductive phase duration, the high source to sink ratio and the high dry matter stem remobilization capacity, as well as the low seed number and high seed weight are intrinsic characteristics of the high protein genotype that could be associated with its high seed protein content and concentration and yield under stressful conditions. This knowledge is key to develop soybean management strategies to improve seed protein level and yield under contrasting productive scenarios.
The effects of initial and subsequent overweight or obesity on hypertension in the middle age
Authors: Pan Pei , Yang Ying , He Sen , Zhao Gang , Chen Xiao‐Ping
Published: March 2024

The aims of our study were to examine whether initial or subsequent adiposity status had a greater effect on hypertension. We collected data in 1992 and again in 2007 from the same group of 597 individuals in the middle age. The subjects were classified into four groups: individuals with a normal body mass index (BMI) in 1992 and 2007 were in Group I; those with a normal BMI in 1992, but became overweight or obese in 2007 were in Group II; those who were overweight or obese in 1992, but had a normal BMI in 2007 were in Group III; and those who were overweight or obese in 1992 and 2007 were in Group IV. Their demographic data were recorded. The relationship between adiposity status and hypertension was analyzed using logistic regression model. The cumulative incidence of hypertension was 35.5%, 56.3%, 50.0%, and 65.1% for Group I to IV, respectively. Compared with Group I, after adjusted factors, the hazard ratio (HR) was 1.80 for Group II (P = .001), 1.40 for Group III (P = .150), and 2.31 for Group IV (P < .001). Adiposity status in 2007 could predict hypertension (OR = 2.5, P < .001), as opposed to the initial adiposity status (P = .148). Subsequently adiposity status could have major effects on hypertension. Our society is very short of public health resources, particularly in developing countries, we should pay more attention to current adiposity status and encourage middle‐aged people to lose weight.
Comparisons of an automated oscillometric device with a hybrid manual auscultatory device for the Korea National Health and Nutrition Examination Survey
Authors: Kim Yu‐Mi , Park Sang Hyeon , Shin Jinho , Sung Ki‐Chul , Ihm Sang‐Hyun , Kim Seong Heon , Kim Dae‐Hee , Park Sang Min , Cho In Jeong , Oh Kyung Won , Lee Eun Mi
Published: March 2024

This study evaluated an oscillometric device (OD), Microlife WatchBP Office AFIB, and a hybrid manual auscultatory device (AD), Greenlight 300TM, to determine a suitable blood pressure (BP) measurement device for the Korea National Health and Nutrition Examination Survey in a mercury‐free context. Adhering to the 2018 Universal Standard's suggested consensus, the study involved 800 subjects (mean age 51.2 ± 17.5 years; 44.3% male), who underwent triplicate BP measurements following 5 min of rest in a randomized order (OD‐first: 398 participants; AD‐first: 402 participants). BP difference was calculated as OD value minus AD value, with results stratified by measurement sequence. The overall BP difference and tolerable error probability were ‐1.1 ± 6.5/‐2.6 ± 4.9 mmHg and 89.2%/92.5% for systolic/diastolic BP (SBP/DBP), respectively. Lin's concordance correlation coefficient was 0.907/0.844 for SBP/DBP (OD‐first/AD‐first: 0.925/0.892 for SBP, 0.842/0.845 for DBP). The overall agreement for hypertension (BP ≥ 140 and/or 90 mmHg) was 0.71 (p < 0.0001), and the OD underestimated the overall hypertension prevalence by 5.1%. Analysis of the AD‐first data revealed a lower level of agreement compared to the OD‐first data; however, the observed blood pressure difference adhered to Criterion 1 of the 2018 Universal Standard. Microlife met the Criterion 1 of 2018 Universal Standard but underestimated the prevalence of hypertension. The BP discrepancy increased with higher BP levels, male sex, and smaller AC. With increasing age, the discrepancy decreased for SBP and increased for DBP.
ReThinking clinical reasoning: A paradigm shift
Authors: Monteiro Sandra , Keuhl Amy , Lee Mark , Chan Teresa M.
Published: March 2024

Numerous studies have demonstrated that our healthcare systems and medical education programs are fundamentally flawed. In North America and Europe, most systems were built upon values and structures that have historically benefitted middle and upper class males of European descent in the global north. As a result, there continue to be systemic biases that are pervasive throughout our healthcare systems and medical education programs. This has led to inequities in health outcomes and clinical reasoning practices which marginalize several communities. These biases are perpetuated as we continue to lead medical education research and practice with traditional values and views of evidence. To address these issues, we proposed a ‘flipped’ conference in which three interdisciplinary writing teams, comprised of both junior and senior academics, clinicians, and researchers, were invited to rethink the foundations of clinical reasoning. In the months leading up to the conference, each writing team explored a specific topic related to clinical reasoning and racial equity. The papers, presented during the virtual conference are now available in this issue of the Journal for the Evaluation of Clinical Practice. In addition, 6 more publications were added to this special topic to showcase new evidence and theory that builds on the recommendations in the three core papers.