bluuebunny/crossref_metadata_2025 huggingface dataset:
Ethylene negatively regulates peach gummosis caused by Lasiodiplodia theobromae by modulating the jasmonic, salicylic acid and UDP-sugar metabolism
Authors: Zhang Dongmei , Zhang He , Huang Xue , He Hanzi , Ye Junli , Cardinale Francesca , Liu Junwei , LI GUO
Published: January 2024

Peach gummosis caused by Lasiodiplodia theobromae is one of the most
detrimental diseases in southern China. This disease is characterized
with necrosis and gum exudation in shoots. Ethylene (ET) is known to
induce gum formation, however its role in defense against peach fungal
gummosis and regulatory mechanism remain unclear. Here, the ET effect on
gummosis progression was investigated in peach shoots treated with ET or
ET inhibitors before L. theobromae inoculation. Peach shoot infection
induced ET production and the transcription of related genes; ET
application increased gum formation whereas ET inhibitors repressed it.
Additionally, ET treatment significantly reduced jasmonic (JA) level and
downregulated OPRs transcript, but increased salicylic acid (SA) content
and upregulated PAL expression. ET application increased arabinose
content, but decreased glucose and fructose levels. Transcripts of genes
related to UDP-sugar metabolism, sugar transporters, and cell wall
degradation were upregulated upon ET treatment, and reduced by ET
inhibitors. Therefore, ET negatively regulates symptom development in L.
theobromae-infected shoots, likely by modulating the JA/SA response and
promoting UDP-sugar metabolism and transport, and cell wall degradation
during gummosis. Our findings shed light on the molecular mechanism by
which ET orchestrates plant defense and gum formation in peach during L.
theobromae infection.
Mitral Mega Mix -- a Case Report
Authors: Sala Alessandra , Denti Paolo , Alfieri Ottavio , Castiglioni Alessandro , Bonis Michele De
Published: January 2024

Background and aim of the study: Atrial myxomas are the most common
primary cardiac tumors and are generally discovered as incidental
findings. When obstructive symptoms become manifest, acute hemodynamic
impairment may occur with the necessity of surgical correction. Results:
We describe a case of a young woman with progressive dyspnea and
syncope, and presence of a huge intracardiac mass. The mass completely
obliterated the mitral valve orifice requiring emergent surgery.
Immediately following excision and at follow-up normal hemodynamics were
restored. Conclusions: Whenever huge intracardiac masses cause
hemodynamic compromise, emergent surgery is necessary. Immediate relief
is seen following excision.
Elevational variability and controls on temperature sensitivity of soil organic matter decomposition in alpine forests of northwestern China
Authors: Kong Junqia , He Zhibin , Longfei Chen , Yang Rong , Du Jun
Published: January 2024

Patterns and elevational controls on the response of soil organic matter
(SOM) decomposition to temperature in alpine forest soils are critical
to efforts to quantify the regional carbon cycle-climate feedback, but
are not well known. Here, we report rates of soil organic matter (SOM)
decomposition (Rs) and temperature sensitivity (Q10) determined in a
short-term laboratory incubation with a gradual warming from 5°C to 29°C
of soils from different elevations in the Qilian Mountains, China
(2,600, 2,800, 3,000, and 3,200 m). The results showed the Rs
significantly increased with increasing elevation (P<0.001).
Across all elevations, RS first showed an increasing trend at
temperatures < 20 ℃ and then declined substantially, most
likely in response to the content of labile C (greater at the start of
incubation, and declining over time). Q10 of SOM decomposition increased
significantly with increasing elevation and deceasing incubation
temperature (P<0.001). More importantly, soil organic carbon
(SOC), total nitrogen (TN), 1-2 mm aggregate-associated OC, and
elevation were the main control factors affecting Rs and Q10. These
results indicate that high-altitude soils in alpine forests of the
Qilian Mountains are relatively more sensitive to temperature changes,
and have greater potential to release CO2 due to higher SOC contents and
1-2 mm aggregates-associated OC than low-altitude. The findings could
serve as a reference for how regional C pools may respond to future
warming in alpine forests of the Qilian Mountains.
Heterolateral iliac artery pseudoaneurysm complicating lumbar vertebral body fracture following renal transplantation
Authors: Wei haijun , liu yong , sun xiaolei
Published: January 2024

Pseudoaneurysms following renal transplantation in donor renal-external
iliac artery anastomosis is rare, of which the incidence is less than
1%. We present a case of a common iliac artery pseudoaneurysm, of whom
the patient underwent allogenic renal transplantation at 2 years ago
accompanied by a recurrent fever of up to 39.5°C for one month. Computed
tomography angiography revealed a huge pseudoaneurysm located in the
left common iliac artery. The crevasse was blocked by implanting an
abdominal aortic integrated bifurcation graft and a covered stent in the
left iliac artery. Despite concerns about the feasibility and safety of
deploying a graft in a suspected infected pseudoaneurysm, this case
proved infection is not an absolute contraindication. Endovascular
treatment combined with timely spinal open surgery is a recommended and
appropriate therapeutic intervention.
DETECTION AND GENOTYPING OF PORCINE CIRCOVIRUS TYPE 2d IN SWINE PRODUCTIONS OF THE DEPARTMENT OF TOLIMA, COLOMBIA
Authors: Garcia Heinner Fabian Uribe , Mesa Rafael Suarez , Barragán Iang Rondón
Published: January 2024

Porcine circovirosis is a disease of worldwide distribution caused by
porcine circovirus type 2 (PCV2). The clinical forms include
post-weaning multisystemic wasting syndrome, dermatitis syndrome and
porcine nephropathy, and some reproductive disorders, which have been
collectively named porcine circovirus-associated diseases (PCVAD). In
Colombia, some studies have demonstrated the presence of PCV2 in
different regions, reporting PCV2b as the main genotype. However, the
genotype of PCV2 in the department of Tolima is unknown. The aim was to
determine the presence of PCV2 in swine productions of the department of
Tolima and to characterize the virus genotypically. Blood samples
(n=100) were taken by jugular venipuncture from animals with clinical
signs compatible with PCVAD from 4 municipalities in the department. DNA
was extracted in order to detect PCV2 nucleotides by amplifying the ORF2
(cap gene), using the end-point PCR. Positive samples were sequenced and
submitted to bioinformatic analysis for genotype identification, by
comparison with reference sequences reported in GenBank. PCV2 positive
animals (n= 15) were detected at different stages of production in all
municipalities. The phylogenetic analysis showed that the viruses belong
to the PCV2d genotype with a percentage of identity between 95,9-98,9%.
This genotype has not been reported in the country. The present study
demonstrated the presence of PCV2d in pig farms of the department of
Tolima - Colombia related to PCVAD. In Colombia, more extensive studies
are required to determine the presence of new genotypes and their
epidemiological behavior.
The Management of Aural Foreign Bodies During the COVID-19 Pandemic - Our Experience in 39 Patients
Authors: Ho Garm Chi , Thind Ravneek , Yap Darren , Hunt Alison
Published: January 2024

Aim: During the initial wave of the COVID-19 pandemic, ENT UK published
a clinical guide on surgical prioritisation which suggested that aural
foreign bodies can be removed between one and three months from initial
presentation. This case series aims to investigate the impact of leaving
aural foreign bodies in situ for a prolonged period of time, including
the risk of complications, success rates of subsequent removal attempts
and whether foreign bodies can clear themselves without intervention.
Method: Retrospective study of all aural foreign body referrals to the
ENT emergency clinic over a 6-month period. Results: Thirty-four
patients were identified. The duration of foreign bodies left in-situ
ranged from 1 to 78 days. Four patients suffered from traumatic removal
upon initial attempts, however there were no other significant
complications. First attempts made by non-ENT specialists (68.8%) all
failed and were associated with a high risk of trauma (36.4%). The
chances of successful removal on second attempt (28.6%) reduced
dramatically when compared to the first attempt (52.9%). Two patients
had no foreign body visualised upon second attempt, suggesting it has
cleared itself. A total of 7 patients (20.6%) required removal under
general anaesthesia. Conclusions: Due to the unique circumstances of the
COVID-19 pandemic, this is the first case series to look specifically at
the relationship between duration of aural foreign bodies left in situ
for over 30 days from presentation and the risk of complications. Our
data suggests that prolonged duration did not increase the incidence of
complications.
An atypical case of very early-onset familial adenomatous polyposis associated with focal cortical dysplasia
Authors: Henry Asher , Rajasekaran Vivek , Evans Helen , Strauss Magreet , Patel Rakesh , Greenslade Mark , Roberts Amin , Yap Patrick
Published: January 2024

We describe a female toddler with rectal bleeding from extensive colonic
polyposis. She has epilepsy from infancy attributed to focal cortical
dysplasia. Hepatoblastoma was diagnosed at 13 months of age. Germline
testing detected a pathogenic APC (adenomatous polyposis coli gene)
variant. We discuss the anecdotal management of an atypical, very
early-onset symptomatic Familial adenomatous polyposis (FAP) case, and
the clinical utility of genetic confirmation in such cases. We review
the genotype-phenotype correlation of the APC mutational spectrum, and
the existing evidence supporting the hypothesis that cortical dysplasia
is part of the APC-related spectrum.
Pulmonary cryptococcal infection after COVID-19 recovery:A case report and literature review
Authors: Yan Yusheng , Liao Ximing , Hu Haiyan , Yuan Lv , Tang Yuling
Published: January 2024

Background: Coronavirus disease 2019 (COVID-19) is a respiratory
infectious disease caused by a new coronavirus called severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2). It was first reported
in Wuhan, China at the end of 2019, and quickly developed into a global
pandemic. By reviewing the clinical data of 1 patient with cryptococcus
pneumoniae infection after recovered from COVID-19 and reviewing
relevant literature, the following possible clinical consequences of
COVID-19 were discussed and corresponding relevant management strategies
were proposed. Case presentation: A 64-year-old female patient diagnosed
with COVID-19 was cured and discharged. Follow-up laboratory tests
showed that CD4+ cell count reduced and abnormal CD4+/CD8+ cell ratio,
and a growing solid lesion was found on computed tomography (CT) scan
after two mouths after discharge. Ineffective broad-spectrum antibiotics
treatment and positive cryptococcal capsular polysaccharide antigen
tests results indicating the suspicious invasive pulmonary fungal
infection due to immunodeficiency. After anti-fungal treatment, the
right lung lesion were significantly absorbed. Conclusion: After
recovery from COVID-19, T cell count decline may occur, accompanied by
host immune dysfunction. Clinicians should pay more attention to
secondary infections caused by weakened immunity of COVID-19 patients
after recovery, whom might be vigilant against opportunistic pathogens
such as Cryptococcus. Key words: COVID-19, invasive fungal infection,
Cryptococcus, lymphopenia.
Leadless pacemaker implantation for a super-elderly woman with a mediastinal tumor
Authors: Kabutoya Tomoyuki , Aoyama Yutaka , Toriumi Shinichi , Yokota Ayako , Komori Takahiro , Kario Kazuomi
Published: January 2024

A 95-year-old woman with no prior cardiac history presented with
symptomatic complete atrioventricular block. She underwent temporary
cardiac pacing via the cervical vein, but a pacing lead could not be
introduced via the usual route because of a mediastinal tumor. A
leadless pacemaker (MicraTM; Medtronic, Minneapolis, MN) was implanted
on the right ventricular septum via the right femoral vein. The
procedure time was 40 minutes without any complication. The implantation
of a leadless pacemaker was useful to improve the symptoms of a
super-elderly woman with mediastinal tumor.
Salvage hematopoietic stem cell transplantation with tumor-ablative conditioning regimen for nonremission refactor/relapsed acute leukemia in children
Authors: luo rongmu , tian zhengqin , zhang xiaomei , man qihang , gu wenjing , wang jingbo
Published: January 2024

Background: The outcome of hematopoietic stem cell transplantation
(HSCT) in children and adolescents with refractory or relapsed acute
leukemia in nonremission is poor. However, It is uncertain whether an
intensitive conditioning regimen can improve the outcomes or not . This
retrospective analysis aimed to investigate the outcomes of HSCT with
tumor-ablative conditioning regimen (TAC), the safety and efficiency of
HSCT using TAC in nonremission acute leukemia . Procedure: Data from the
Beijing Aerospace Central Hospital on 44 patients with nonremission
acute leukemia (aged <18 years, with blasts 5-98% in bone
marrow) who had undergone HSCT between January 2016 and December 2018
were evaluated. Results: Overall, 32 patients died. The median follow up
was 1330 days in survival patients, and the 3-year OS and LFS were
27.3%( 95% CI, 14.2% to 40.4% ) and 28.9%（95% CI, 15.4% to
42.4%）. The cumulative incidence of relapse is 59.1% (95% CI, 44.6%
to73.6) and non-relapse mortality (NRM) is 18.1%, 95 CI,
12.4%-23.8%). Multivariate analysis showed that relapse was an
independent factor affecting OS (HR=4.308 (95% CI:
1.820-10.186,P=0.001) Conclusion: this 3-year retrospective analysis
found that salvage HSCT with TAC is an alternative selection on
nonremission leukemia, the TAC regimen is a safe and effective regimen.
The relapse is still the main cause of death. A larger prospective study
is needed to verify the promising study.
A case of neuropsychiatric systemic lupus erythematosus with neurogenic pulmonary edema
Authors: li Rongqi , Wang Yingai , Wu Xiuhua , Wang Junping , Wei Wei , Li Xin
Published: January 2024

We reported a 21-year-old female patient with systemic lupus
erythematosus, accomplicated with posterior reversible encephalopathy
syndrome and neurogenic pulmonary edema. After hormone pulse and
antiepileptic treatment, the patient’s consciousness recovered, and the
reexamination CT and cerebrospinal fluid examination were basically
normal. There was no recurrence later.
Identification of anomalous origin of left circumflex coronary artery from the right sinus of valsalva by transthoracic echocardiography
Authors: Jha Abhishek , Ostwal Kunal , Akash Advait , Velamakanni Subrahmanya Murti , Panchal Shivam , Patel Tejas
Published: January 2024

Anomalous origin of the left circumflex artery from the right sinus of
Valsalva with a retro-aortic course is a relatively common abnormality.
Although considered benign, the retro-aortic course of the anomalous
vessel may be an important consideration in aortic surgery and device
closure of atrial septal defects. 2D transthoracic echocardiography can
easily help detect this anomalous course in a non-invasive manner. Here,
we present the echocardiographic images of a young patient who had come
for evaluation of chest pain.
PVP-I reduces LPS-induced airway inflammation by blocking TLR4 signaling in airway epithelial cells
Authors: Lee Seung Hoon , Yeon Sun-Hee , Choi Seung-Hyeon , Park Soo-Kyoung , Choi Mi Ra , Kim Yong-Min
Published: January 2024

Background: Povidone-iodine (PVP-I) is an antiseptic and a disinfectant
with broad-spectrum antimicrobial activity against various pathogens.
However, it is unclear whether PVP-I nasal instillation can suppress
mucosal inflammation in non-eosinophilic chronic rhinosinusitis (CRS)
mice. Objective: This study aimed to explore the anti-inflammatory
effects and underlying molecular mechanism of PVP-I on
lipopolysaccharide-stimulated airway epithelial cells and investigate
whether nasal instillation of PVP-I can suppress mucosal inflammation in
non-eosinophilic CRS mice. Methods: Inflammation-related molecules in
the nasal epithelial cells and non-eosinophilic CRS mice were measured
by enzyme-linked immunosorbent assay, western blotting, quantitative
real-time polymerase chain reaction, immunoprecipitation, and
histopathological analysis (hematoxylin and eosin staining,
immunohistochemistry, and periodic acid‑schiff staining). Results: PVP-I
blocked expressions of various inflammation-related molecules, such as
NLRP3, NF-κB-p65, caspase-1, and IL-1β; induced translocation of NF-κB
to the nucleus; and promoted assembly of NLRP3/ASC complexes in the
nasal epithelial cells and non-eosinophilic CRS mice. Notably, PVP-I
strongly blocked the receptor interaction of TLR4 and MyD88 in the
epithelial cells of nasal mucosa. Conclusion: We demonstrated that PVP-I
significantly attenuated inflammatory molecules and cytokines via
blocking the formation of TLR4 and MyD88 complexes during LPS-induced
mucosal inflammation in non-eosinophilic CRS.
Efficacy of combination therapy with biofeedback and dapoxetine in lifelong premature ejaculation treatment: A prospective randomized study
Authors: Cinislioglu Ahmet , Yılmaz Ali , Demirdogen Saban , Yilmazel Fatih Kursat , Sam Emre , Karabulut İbrahim , Koç Mahmut , Özbey İsa
Published: January 2024

Aims: Premature ejaculation (PE) is a common sexual dysfunction that
significantly affects the quality of life of the patient and their
partner. We aimed to compare efficacy and safety of the combination
therapy with biofeedback-guided pelvic floor exercise therapy (BFT) and
dapoxetine 30 mg. Results: Patients were divided into three groups as
BFT, dapoxetine 30 mg and combination with BFT and dapoxetine 30 mg. The
patients were compared with the intravaginal ejaculatory latency time
(IELT) pre-treatment and post-treatment 1st and 3rd months. The mean
IELTs of the patients in Group 1 were 40 sec in pre-treatment, 115 sec
at the end of the 4th week and 140 sec at the end of the 12th week. The
IELT values of the patients in Group 2 were 40 sec in pre-treatment, 145
sec in the 4th week and 170 sec in the 12th week . The IELT values were
calculated in Group 3 as 42.5 sec in pre-treatment, 185 sec in the 4th
week and 205 sec in the 12th week When the IELT was statistically
compared between the groups at 1st and 3rd months, the duration in
combination group was found to increase significantly (p <
0.001). Discussion: Pelvic floor muscles play an important role in
sexual function. Rehabilitation of pelvic floor muscles provides
two-stage efficiency with electrostimulation and physiokinesitherapy; it
aims to improve the contractile forces of the perineal muscles and
increase the urethral sphicter closure force. Besides, the
rehabilitation of pelvic floor muscles allows patients to control the
contractions of the right muscle group accompanied by biofeedback
Conclusion: Combination therapy with BFT and dapoxetine 30 mg in
lifelong PE treatment is a good alternative with low side effect profile
and acceptable continuous efficiency
Linkage and association mapping identifies loci and develops kompetitive allele specific PCR markers for improving wheat grain protein content
Authors: Jiang Peng , Zhang Peng , Wu Lei , He Yi , Li Chang , Ma Hongxiang , Zhang Xu
Published: January 2024

Wheat grain protein content (GPC) is an important quality indicator. The
GPC of wheat grown in the middle and lower reaches of the Yangtze River
is often low. Marker-assisted selection (MAS) is an effective tool for
improving quantitative traits; however, except Gpc-B1, most markers have
not been effectively applied in GPC improvement, although many related
loci have been identified. Linkage analysis using a recombinant inbred
line population from the cross of core parents of Ningmai 9 and Yangmai
158 and association mapping using the local cultivated varieties were
performed and nine candidate intervals were identified. The appropriate
kompetitive allele specific PCR (KASP) markers associated with GPC were
successfully developed and applied in 1163 F4 breeding lines. Three
markers, Kgpc-2B, Kgpc-2D, and Kgpc-4A, were validated to be
significantly related to GPC by large-scale association mapping, and
they were combined to achieve the highest efficiency to enhance GPC. We
applied these markers in 164 F6 breeding lines and obtained 15 lines
with high GPC, indicating their high selective efficiency. Further,
strategies for gene exploration in the three significant intervals were
proposed. These results were expected to provide a novel route for
improving GPC in wheat quality breeding.
Salivary parameters and their correlation with absolute neutrophil counts in children undergoing chemotherapy for Acute lymphoblastic leukemia: A Prospective Cohort Study
Authors: Mishra Richa , Kapur Aditi , Trehan Amita , Goyal Ashima , Gauba Krishna
Published: January 2024

Background: Chemotherapy is the mainstay of treatment for Acute
Lymphoblastic Leukemia (ALL) and is a phased treatment modality to
counter malignant cells. Although chemotherapy significantly improves
the disease prognosis, its adverse effects are also manifold, including
oral consequences, owing to cytotoxic damage. Methods: The present study
was conducted to evaluate alterations in salivary flow rate, pH and
buffering capacity at baseline, post Induction and post Consolidation
phases and to correlate these parameters with Absolute Neutrophil Counts
in a cohort of 43 patients, aged 3-12 years. Results: A decrease in the
salivary parameters was observed at the end of Induction phase, with a
statistically significant decrease in unstimulated salivary flow rates
(p<0.01). Some improvement was found at the end of
Consolidation phase; however, all the parameters did not reach their
baselines values. Statistically significant positive correlations were
found between ANC and salivary flow rate(p=0.005), pH(p<0.00)
and buffering capacity(p<0.00). Conclusion: The positive
correlations of salivary parameters with ANC of the subjects may be
considered indicative of a concomitant immunological compromise in
children with a reduction in salivary parameters.
Clinical characteristics of Castleman disease in head and neck
Authors: WU Jun , LI Jingkun , WU Xi , MA Lijuan
Published: January 2024

Objective: This study was performed to investigate the clinical and
pathological manifestations of Castleman disease in head and neck, and
to summarize its clinical characteristics, experiences of diagnosis and
treatment, and prognosis of patients. STUDY DESIGN: Retrospective study.
SETTING: Hunan Provincial People’s Hospital. Methods: We retrospectively
analyze the clinical data of 8 patients suffering from Castleman disease
in head and neck who received treatment in Hunan people’s Hospital
between 2013.1 to 2020.07, and review the relevant literature. Results:
7 of the 8 patients were characterized by single or multiple painless
masses in head and neck. They were categorized as unicentric Castleman
disease (UCD). Case 8 was clinically classified as multicentric
Castleman’s disease (MCD) because the neck mass of this patient was
found with discomfort such as anemia, fatigue and fever. The
pathological types were hyaline-vascular type (HVT) in 6 patients, mixed
type in 1 patient and the plasma cell type (PCT) in 1 patient. All the
patients were operated to remove the mass and showed no recurrence in
the following up for 4-93 months. Conclusions: The diagnosis of
Castleman disease in head and neck should be confirmed by pathological
examination. UCD is the main type in the clinical classification, and
HVT is the main type in pathological. Surgical resection of the mass can
be an effective treatment, and proper medical therapy should be
supplemented to those with postoperative recurrence. The patients with
UCD generally have a good prognosis. [KEY WORDS] Head and neck;
Castleman disease; Diagnosis; Treatment
Role of Placenta fibrinogen-like 1 (FGL1) in the Pre-Eclampsia progression: a functional study
Authors: Cheng Tsung-Lin , Chen Chung-Hwan , Wu Meng-Hsing , Lai Chao-Han , Lee Ko-Hung , Lin Sheng-Hsiang , Shiau Ai-Li , Wu Chao-Liang , Lin Kang
Published: January 2024

Objective: To study the role of placenta fibrinogen-like 1
(FGL1) during pre-eclampsia (PE) progression. Design: A
case-control study combined with the experimental research of the
cellular and PE mouse model. Setting: FGL1 is a protein
involved in liver regeneration, but its role in the placenta and PE
remains uninvestigated. Population or Sample: Serum and
placenta from the PE mouse model and in women with (n = 38) and without
(n = 42) PE were analyzed. Methods: Pregnant C57Bl/6 mice (n =
6) were administered L-NAME subcutaneously with or without FGL1 once
daily starting on day 7–14 of pregnancy until sacrifice. Maternal body
weight, blood pressure, urinary protein, weight and length of the
placenta and fetus were assessed. The placental structure was evaluated
using histochemistry staining. The sera of pregnant women during the
late trimester were quantified with ELISA. Main Outcome
Measures: FGL1 expression in serum and placenta during PE, and
treatment outcome of FGL1. Results: FGL1 expression in both
serum and placenta was significantly upregulated in patients with PE and
mice compared with control groups. FGL1 treatment decreased maternal
hypertension, and proteinuria, ameliorated fetal weight in PE mice,
downregulated proinflammatory cytokines (interleukin-1b and -6), and
maintained the balance between antiangiogenic (sFlt-1, soluble fms-like
tyrosine kinase-1) and proangiogenic substances (Pgf, placental growth
factor) in the placenta. Conclusions: Placental FGL1
upregulation plays a pivotal role in reducing PE progression.
Funding: Ministry of Science and Technology (MOST
106-2314-B-006-066, 108-2314-B-006-062), Taiwan; National Cheng Kung
University Hospital (NCKUH-10604002, NCKUH-10705015, NCKUH-10902008);
Kaohsiung Medical University (KMU-TC108A02, KMUH-DK(A)110003)
QSAR-QMMM Cryptographic Mining on Chern-Simons Topological Geometrics for the generation of a Ligand Targeting COVID-19-SARS-COV-2 SPIKE D614G Binding Sites.
Authors: Grigoriadis Ioannis
Published: January 2024

SARS coronavirus 2 (SARS-CoV-2) in the viral spike (S) encoding a
SARS-COV-2 SPIKE D614G mutation protein predominate over time in locales
revealing the dynamic aspects of its key viral processes where it is
found, implying that this change enhances viral transmission. It has
also been observed that retroviruses infected ACE2-expressing cells
pseudotyped with SG614 that is presently affecting a growing number of
countries markedly more efficiently than those with SD614. In this
paper, we strongly combine topology geometric methods targeting at the
atomistic level the protein apparatus of the SARS-COV-2 virus that are
simple in machine learning anti-viral characteristics, to propose
computer-aided rational drug design strategies efficient in computing
docking usage, and powerful enough to achieve very high accuracy levels
for this in-silico effort for the generation of the AI-Quantum designed
molecule of Combination of GisitorviffirnaTM, Roccustyrna_gs1_TM, and
Roccustyrna_fr1_TM ligands with Preferred IUPAC Names of
(7aR)‐5‐amino‐N‐[(S)‐{2‐[(S)‐[(E)‐(amino methyl
idene)amino](cyano)methyl]hydrazin‐1‐yl}(aziridin‐1‐yl)phosphoryl]‐1‐[(2E)‐2‐[(fluoromethanimidoyl)imino]acetyl]‐7‐oxo‐1H,7H,7aH‐pyrazolo[4,3‐d]pyrimidine‐3‐carboxamide;
N‐{[(2‐amino‐ 6‐oxo‐6,9‐dihydro‐1H‐purin‐9‐yl)amino]({1‐[5‐
({[cyano({1‐[(diaminomethylidene)amino]ethenyl})amino]oxy}methyl)‐3,4‐dihydroxyoxolan‐2‐yl]‐1H‐1,2,4‐triazol‐3‐yl}formamido)phosphoryl}‐6‐fluoro‐3,4‐dihydropyrazine‐2‐carboxamide;[3‐(2‐amino‐5‐sulfanylidene‐1,2,4‐triazolidin‐3‐yl)oxaziridin‐2‐yl]({3‐sulfanylidene‐1,2,4,6‐tetraazabicyclo[3.1.0]hexan‐6‐yl})phosphoroso1‐(3,4,5‐trifluorooxolan‐2‐yl)‐1H‐1,2,4‐triazole‐3‐carboxylate
targeting the COVID-19-SARS-COV-2 SPIKE D614G mutation using
Chern-Simons Topology Euclidean Geometric in a Lindenbaum-Tarski
generated QSAR automating modeling and Artificial Intelligence-Driven
Predictive Neural Networks.
Quantitative Fluorescent Polymerase Chain Reaction for prenatal diagnosis and its application in Vietnam: A review
Authors: Phan Thuan
Published: January 2024

Early identification of fetal abnormalities is a huge challenge for
modern obstetrics. Quantitative fluorescent polymerase chain reaction
(QF-PCR) has quickly become an effective means of chromosome anomaly
detection due to its advantages in terms of timing, manpower and
accuracy. The QF-PCR results also make a significant change in
clinicians’ attitude to some extent, assisting them providing parents
with professional and valuable advice on pregnancy management. In this
review, the advantages and drawbacks of QF-PCR will be explored. By
reviewing studies published in Vietnamese Medical Journals, we conclude
QF-PCR can become a potential screening test in the field of prenatal
diagnosis.
Optimization of synthesis of (S)-omeprazole catalyzed by soybean pod peroxidase in water-in-oil microemulsions using RSM
Authors: Tang Hui , Zhang Yuanyuan , Deng Yashan , Du Shanshan , Li Depeng , Wang Zhiyong , Li Huiling , Gao Xin Xin , Wang Fanye
Published: January 2024

Response surface methodology (RSM) was used to optimize the oxidizing
the omeprazole sulfide to (S)-omeprazole catalyzed by environmentally
friendly catalyst soybean pod peroxidase (SPP) in cetyltrimethylammonium
bromide (CTAB)/isooctane/n-butyl alcohol/water water-in-oil
microemulsions. With the initial concentration of SPP of 3200 U ml-1,
the conversion of the omeprazole sulfide, the (S)-omeprazole yield and
ee were 93.75%, 91.56% and 96.08%, respectively, under the optimal
conditions: Wo of 15.85, the concentration of H2O2 of 22.44 mM and
reaction temperature of 49.68 °C, respectively. The proposed mechanism
of asymmetric sulfoxidations catalyzed by SPP involves three concomitant
mechanisms as follows: (1) a two-electron reduction of SPP-I, (2) a
single-electron transfer to SPP-I and (3) nonenzymatic reactions,
including five enzymatic and two nonenzymatic reactions, which is
reasonable and can express the oxidations. With 5.44% of the average
relative error, a kinetic model based on the mechanisms fitting observed
data very well was established, and the SPP-catalyzed reactions
including both the two-electron reduction and the single-electron
transfer mechanisms obey ping-pong mechanism with substrate and product
inhibition, while nonenzymatic reactions follow a power law. This study
has also demonstrated the feasibility of SPP as a substitute with low
cost, excellent enantioselectivity and better thermal stability.
Body Piercing with a Metallic Tongue Ring Resulting in Ineffective ICD Shocks: “Heart to Mouth”
Authors: Singh Manavotam , Brar Vijaywant , Alexander Nebu , Tashayyod Rustin , O'Donoghue Susan , Worley Seth
Published: January 2024

Left ventricular assist devices (LVADs) provide circulatory support to
patients with severe left ventricular systolic dysfunction. Many such
patients have a pre-existing implantable cardioverter defibrillator at
the time of their LVAD surgery. LVAD implantation can alter ICD lead
parameters including R wave sensing, RV capture threshold, and
impedance. These changes can in turn affect the ability of the ICD to
successfully treat malignant ventricular arrythmias. In most patients
who present with ineffective ICD shocks, the failed shock is assumed to
be secondary to the patient’s severe cardiomyopathy. Especially the role
of physical examination in such patients is often minimized. In our
patient a thorough history and history guided physical examination, led
us to the root cause of his failed ICD shocks. Our patient was noted to
have a metal tongue ring, which was the likely cause of his ineffective
ICD shocks. Our case highlights the importance of a comprehensive
history and physical examination.
INCIDENCE OF CONGENITAL UTERINE ABNORMALITIES IN POLYCYSTIC OVARIAN SYNDROME
Authors: Aslan Kiper , Albayrak Ozge , Orhaner Aylin , Kasapoglu Isil , Uncu Gurkan
Published: January 2024

Objective: To determine the frequency of uterine abnormalities in PCOS
patients compared to normo-ovulatory patients and their relationship to
androgen levels. Design: Retrospective cohort study. Setting: Tertiary
University Hospital Population: Total of 103 women, 51 PCOS, and 52 male
factor infertility Interventions: HSG images of all patients were
numbered and evaluated by ten senior reproductive endocrinologists
blinded to the research and chose the most appropriate figure for each
patient’s HSG image and marked the shape of cavity according to the ASRM
and ESGE/ESHRE classification. Main outcome measures: Abnormal HSG
Rates, serum AMH and androgen levels Results: The percentage of the
normal uterine cavity was significantly lower in the PCOS group than
control group as 51 % and 66.7 %, 77 % and 94.2 % according to the
ASRM and ESHRE/ESGE classifications, respectively. The frequency of
Partial Septate Uterus (ESHRE-ESGE U2a / ASRM Va, 21.6% vs 5.8 %
p<0.05), Complete Septate Uterus (ESHRE-ESGE U2b / ASRM Vb,
7.8% vs 0% p<0.01) and Dysmorphic Uterus (ESHRE-ESGE U1c) /
ASRM VII) 3.9 % vs 0 p<0.05) were significantly higher in
PCOS patients than controls. The frequency of arcuate uterus classified
as VI in ASRM and considered normal in ESHRE/ESGE was 16 % and 17.3 %
in PCOS and control groups, respectively, without statistically
significant difference. The increased frequency of intrauterine cavity
abnormalities was correlated with serum-free testosterone levels.
Conclusion: This study provides that the frequency of uterine cavity
abnormality is clearly higher in PCOS patients compared to the healthy
population.
A renal transplant patient with covid-19 disease presented with acute cellular rejection
Authors: Soleimani Neda , Mohammadzadeh Sahand
Published: January 2024

A 27-year-old underwent a diseased kidney transplant. Three days after
transplantation COVID-19 was diagnosed for our patient.
Immunosuppressants were reduced and a renal biopsy was done which showed
acute T cell-mediated rejection. We aimed to report a case to help
clinicians better understand the risks to kidney transplant recipients.
Dysphagia Rehabilitation Interventions in Moderate-Severe Acquired Brain Injury: A Scoping Review
Authors: Benton Brooke , McIntyre Amanda , Mirkowski Magdalena , Faltynek Pavlina , Muise Stephanie , Gurgel-Juarez Nalia Cecilia , Teasell Robert
Published: January 2024

Objectives: To conduct a scoping review of the available literature and
identify existing interventions for dysphagia rehabilitation among
individuals with moderate to severe ABI during the acute and chronic
phases of recovery. Design: Scoping review involving a literature search
of multiple databases for studies published in English up to July 2018.
Inclusion criteria: (1) moderate-severe ABI, (2) participants aged 18+
years, and (3) a dysphagia rehabilitation intervention was provided. The
Physiotherapy Evidence Database (PEDro) tool was used to determine
methodological quality. Results: 17 studies met inclusion criteria; nine
of which had <50% ABI participants, four had
>50% ABI, and four did not specify ABI percentage. Twelve
studies were published between 2012-2018, and five were published
between 1990-2007. Fifteen journal articles and two conference abstracts
met inclusion. Five randomized controlled trials (RCTs) were included,
two level 1b evidence, two level 2 evidence, and one was of unknown
quality. Four prospective controlled trials (PCTs) provided level 2
evidence. Three post-test and three pre-post studies provided level 4
evidence, and two case reports provided level 5 evidence. Nine different
interventions were investigated, with electrical stimulation,
individualized management programs, and diet manipulation being the most
common. Eleven unique outcome measures were used overall, which crossed
several domains. Conclusions: The literature investigating dysphagia
rehabilitation interventions for ABI, the vast majority of which are
traumatic brain injury is limited, with wide variability in intervention
type, study design, injury etiology, and outcome assessment across
studies. There remains an important evidence gap for ABI dysphagia
rehabilitation.
A Three Fractional Order Jerk Equation With Anti Periodic Conditions
Authors: DAHMANI Zoubir , BELHAMITI Meriem Mansouria , Sarikaya Mehmet Zeki
Published: January 2024

We study a new Jerk equation involving three fractional derivatives and
anti periodic conditions. By Banach contraction principle, we present an
existence and uniqueness result for the considered problem. Then, by
applications of Krasnoselskii fixed point theorem, another result for
the existence of at least one solution is established. Also, An
illustrative example is discussed. At the end, an approximation for
Caputo derivaitive is proposed and some chaotic behaviours are discussed
by means of the Runge Kutta 4th order method.
Diffusion MRI of Parathyroid Adenomas using a Distortion Reduction Scheme
Authors: Yamamoto Hirotaka , Iima Mami , Kishimoto Yo , Kishimoto Ayami , Koyasu Sho , Yamamoto Akira , Kawai Yoshitaka , Yoshizawa Akira , Omori Koichi
Published: January 2024

Objectives: To evaluate whether readout-segmented echo-planar imaging
(RS-EPI) diffusion-weighted imaging (DWI) can reduce image distortion
and improve the lesion identification in parathyroid adenomas (PTAs)
compared to single-shot EPI (SS-EPI) DWI, and to determine whether PTAs
can be differentiated from other soft tissue structures of the head and
neck region by using the apparent diffusion coefficient (ADC) value.
Design: Retrospective study. Setting: Kyoto University Hospital.
Participants: 24 patients who underwent a magnetic resonance imaging
(MRI) scan for the preoperative diagnosis of PTAs from January 2012 to
March 2020 were enrolled. Main outcome measures: MRI was performed with
the acquisition of a 1.5T MR imaging system (Avanto or Trio, Siemens,
Erlangen, Germany) or a 3T MR imaging system (Prisma or Skyra, Siemens).
Image quality and distortion of PTAs on RS-EPI and SS-EPI was evaluated
using a reported five point score, and the ADC values of PTAs, thyroid
glands and cervical neck lymph nodes were compared. Results: RS-EPI
provided significantly less distortion but did not improve the lesion
identification compared to SS-EPI. On SS-EPI, the PTAs had significantly
higher ADC values compared to the cervical lymph nodes. On RS-EPI, the
PTAs had significantly higher ADC values compared to the thyroid glands
and cervical lymph nodes. Conclusions: RS-EPI reduces the DWI distortion
in PTAs. The ADC value obtained using RS-EPI enables the differentiation
of PTAs from nearby structures, such as thyroid glands and cervical
lymph nodes.
Smoking Cessation Rates of Patients with Bladder Cancer and Its Effect on Oncological Outcomes
Authors: Akgul Murat , Dogan Cagri , Yazıcı Cenk Murat , ŞAHİN Mehmet , Ozcan Rıdvan
Published: January 2024

ABSTRACT BACKGROUND: Smoking is the major risk factor for development of
bladder cancer (BC). We evaluated the rate and the time of cessation of
smoking in patients with BC and analysed the effect of ongoing smoking
at recurrence and progression. METHODS: All patients were informed at
the time of BC diagnosis about the correlation between smoking and BC
and strictly warned to quit smoking. The demographic properties,
pathologic characteristics and smoking status of the patients were
evaluated retrospectively. Both the patients and the family members were
questioned to evaluate the smoking status of the patient during the
follow-up period. The disease recurrence and progression was correlated
with the habitual attitude of patients in terms of smoking status.
RESULTS: A total of 245 patients were included to the study. The mean
follow-up period was 37.3±27.8 months (7-143 months). There were
102(41.6%) patients who were smoker and 143(58.4%) patients who were
non-smoker at the time of diagnosis. Among the smoker patients,
34(33.3%) stopped smoking after the diagnosis of BC. The median smoking
cessation time was 1.5 months and 64.7% of these patients stopped
smoking in the first six months after the diagnosis. The Cox regression
model did not show any relationship between the smoking status and
recurrence/progression. CONCLUSION: The rate of cessation of smoking at
BC patients was low. The first months of the diagnosis were the most
suitable period for the patients to stop smoking. The smoking status
after the diagnosis was not related with the tumor recurrence and
progression.
Congenital deficiency of conventional dendritic cells promotes atopic dermatitis-like inflammation in mice
Authors: Nishikawa Yotaro , Fukaya Tomohiro , Fukui Takehito , Uto Tomofumi , Takagi Hideaki , Nasu Junta , Miyanaga Noriaki , Riethmacher Dieter , Choijookhuu Narantsog , Hishikawa Yoshitaka , Amano Masahiro , Sato Katsuaki
Published: January 2024

Background: Atopic dermatitis (AD) is a common pruritic inflammatory
skin disease characterized by impaired epidermal barrier function and
dysregulation of T heper-2 (TH2)-biased immune responses. While the
lineage of conventional dendritic cells (cDCs) are implicated to play
decisive roles in T-cell immune responses, their requirement for the
development of AD remains elusive. Therefore, we sought to determine the
impact of the constitutive loss of cDCs on the progression of AD-like
inflammation. Methods: CD11c-Cre:ROSA26-diphtheria toxin α chain (DTA)
transgenic (Tg) mice that constitutively lacked CD11chi cDCs and
wild-type (WT) littermates received topical application of a low
calcemic analogue of vitamin D3 known as MC903 (calcipotriol) to compare
the development of AD-like inflammation and immune responses as well as
the colonization of Staphylococcus aureus (S. aureus) in eczematous
skin. Results: Unexpectedly, the congenital deficiency of cDCs not only
exacerbates the pathogenesis of AD-like inflammation but also elicits
immune abnormalities with the increased composition and function of
granulocytes and group 2 innate lymphoid cells (ILC2) as well as B cells
possibly mediated through the breakdown of the Fms-related tyrosine
kinase 3 ligand (Flt3L)-mediated homeostatic feedback loop. Furthermore,
the constitutive loss of cDCs accelerates skin colonization of S.
aureus, that associated with disease flare. Conclusion: cDCs maintains
immune homeostasis to prevent the occurrence of immune abnormalities to
maintain the functional skin barrier for mitigating AD flare.
Comparative analysis of applying an advanced rod monolateral external fixator with the classical method of long tubular bones distraction based on a circular multi-axis system in achondroplasia
Authors: Dossanov Bolatbek , Trofimchuk Vitaliy , Lozovoy Vassiliy , Khmyzov Sergey , Dossanova Assem , Zhukenov Olzhas , Tuktiyeva Nazym , Angelov Aleksandr
Published: January 2024

The work aimed to evaluate the effectiveness of the developed
distraction system based on the rod external monolateral fixation
mechanisms by comparing it with the classical technique of long tubular
bones distraction based on the circular multi-axial system. The study
included patients with a genetically confirmed diagnosis of
achondroplasia. The main group consisted of 14 patients who underwent
surgical limb lengthening by the rod monolateral external fixator with a
distraction system developed by the authors.). In the majority of
patients of the main group the lengthening value close to the planned
one as well as the deformation correction was achieved. The fixation
period was averagely 83.8±3.7 days, the regenerate length was 8.5±0.6
cm, and the mechanical strength of the distraction regenerate was
10.3±2.18º. The fixation time was significantly lower, and the length of
the regenerate was longer compared to that in the control group. The rod
external fixator with a control distraction system developed by the
authors has small dimensions and low weight of the external supporting
elements of high durability. It is reported to provide a good
psychological tolerance of the treatment process and significantly
outperforms the circular multi-axis system.
Rosai-Dorfman Disease: Sinusoidal Histiocytosis with massive lymphadenopathy and Splenic abscess
Authors: Goel Lepsy , . Nitesh , Singh Ragini , Sharma Utkarsh , Sethi Aashish
Published: January 2024

ABSTRACT Rosai-Dorfman disease (RDD) or sinus histiocytosis with massive
lymph-adenopathy is characterized as massive, painless, bilateral,
symmetric cervical lymphadenopathy, with fever, leucocytosis, elevated
sedimentation rate, and hyper-gamma-globulinemia. This paper reports on
the case of a 2-year-old child with history of progressive, bilateral
cervical lymph-adenopathy associated with fever, generalized weakness.
Histo-pathological and immune-histochemistry studies showed
predominantly large histiocytes, emperipolesis and S-100 positivity
suggestive of RDD. Patient presented with splenic abscess on follow up
and managed conservatively. Here we are describing a 2-year old patient
of RDD associated with splenic abscess, currently under strict
follow-up.
Opah (Lampris megalopsis) genome and the genetic mechanisms underlying whole-body endothermy
Authors: Bo Jing , Lv Wenqi , Sun Ning , Wang Cheng , Wang Kun , Liu Pan , Feng Chenguang , Yang Liandong , He Shunping
Published: January 2024

Endothermy (production and retention of metabolic heat) enhances the
physiological functions of mammals, birds and a few fish species. The
opah, Lampris megalopsis (Lampridiformes), is the only known teleost
with whole-body endothermy that confers physiological and ecological
advantages in competition. The genetic mechanisms of opah whole-body
endothermy are unclear. We sequenced the opah genome and assembled a
high-resolution genome. Through analysis of positive selection, rapid
evolution, and family expansion, we discovered several genes that likely
contributed to the development of dark red pectoral muscles, uncoupling
protein sarcolipin mediated heat production, retia mirabilia and adipose
metabolism in opah. A functional experiment showed that the positively
selected glycolysis enzyme LDHM exhibits higher catalytic activity in
energy supply related pathways. The results illustrate possible
molecular and genetic mechanisms involved in whole-body endothermy in
fish.
Time domain analysis for electromagnetic scattering by an elastic obstacle in a two-layered medium
Authors: Wei Changkun , Yang Jiaqing , Zhang Bo
Published: January 2024

In this paper, we consider the scattering of a time-dependent
electromagnetic wave by an elastic body immersed in the lower half-space
of a two-layered background medium which is separated by an unbounded
rough surface. By proposing two exact transparent boundary conditions
(TBCs) on the artificial planes, we reformulate the unbounded scattering
problem into an equivalent initial-boundary value problem in a strip
domain with the well-posedness and stability proved using the Laplace
transform, variational method and energy method. A perfectly matched
layer (PML) is then introduced to truncate the interaction problem with
two finite layers containing the elastic body, leading to a PML problem
in a finite strip domain. We further verify the existence, uniqueness
and stability estimate of solution for the PML problem. Finally, we
establish the exponential convergence in terms of the thickness and
parameters of the PML layers via an error estimate on the
electric-to-magnetic (EtM) capacity operators between the original
problem and the PML problem.
A new variant of Jensen inclusion and Hermite-Hadamard type inclusions for interval-valued functions
Authors: Sial Ifra Bashir , Mei Sun , Ali Muhammad Aamir , BUDAK Hüseyin
Published: January 2024

In this research, for interval-valued functions, we give a new version
of Jensen inclusion which is called Jensen-Mercer inclusion. Moreover,
we establish some new inclusions of Hermite-Hadamard-Mercer type for
interval-valued functions.