bluuebunny/crossref_metadata_2025 huggingface dataset:
Profile of Self-Care Capacity and Alcohol Use in Elderly Brazilians during the COVID-19 Outbreak: An Online Study
Authors: Okuno Meiry F. P. , Ximendes-Silva Diego , Vancini Rodrigo L. , Adjzen Claudia , Andrade Marilia S. , de Lira Claudio A. B. , Nikolaidis Pantelis T. , Gomes dos Santos Rafaela , Weiss Katja , Knechtle Beat
Published: August 2022

Background: Preventive and positive online coping strategies are essential for harm reduction associated with alcohol abuse among older adults in pandemic and social isolation scenarios. The objectives were to examine the relationship between alcohol use/abuse and physical capacity/self-care to perform the physical activities of daily living or impairment of the functional capacity of the elderly in the COVID-19 pandemic. Methods: An online cross-sectional survey was carried out. One hundred and one elderly people in the city of São Paulo, Brazil, participated in a community program. Results: Most participants (52.5%) showed excellent self-care skills. Approximately 12% of participants reported problems related to alcohol use/abuse. There was no association between self-care ability and abuse and probable alcohol dependence. Conclusions: Although most participants have excellent self-care and functional capacity and have not evidenced alcohol use/abuse, health professionals need to systematically provide information to prevent alcohol abuse, especially in scenarios of great emotional distress, such as in a pandemic. In addition, the online meetings held by the UAPI program were shown to be opportunities for social interaction and were essential to minimize the negative effects of the possible presence of alcohol use/abuse and the deteriorating performance of physical activities of daily living during a pandemic outbreak for the elderly.
Long-Term Outcomes of Patients with Primary Brain Tumors after Acute Rehabilitation: A Retrospective Analyses of Factors
Authors: Tay Matthew Rong Jie , Seah Justin Desheng , Chua Karen Sui Geok
Published: August 2022

Although primary brain tumors are relatively rare, they cause significant morbidity and mortality due to the high rates of neurological impairment. The purpose of this study was to examine the physical and functional outcomes of patients with primary brain tumors who had undergone inpatient rehabilitation. This was a retrospective study which recruited 163 patients who had been admitted for inpatient rehabilitation. Rehabilitation outcomes, including the Functional Independence Measure (FIM) and Glasgow Outcome Scale (GOS), were recorded up to 1 year post-discharge. The majority of patients (79.1%) had low-grade (WHO Class I-II) tumors, 35 (21.5%) were diagnosed with GBM and 52 (31.9%) had recurrent brain tumors. Rehabilitation outcomes were sustained, with 125 (76.7%) and 113 (69.3%) patients having a GOS of ≥4 at 6 months and 1 year after discharge, respectively. A GOS of ≥4 at 1 year was negatively associated with high-grade tumors (p < 0.001) and radiotherapy (p = 0.028), and positively associated with a higher discharge FIM motor score (p < 0.001) and the presence of a caregiver after discharge (p = 0.034). Our study demonstrates significant positive functional benefits from 4 weeks of inpatient neuro-oncological rehabilitation for patients with primary brain tumors, as well as the importance of supportive care from caregivers.
Transcriptome Analysis of Sugarcane Young Leaves and Protoplasts after Enzymatic Digestion
Authors: Zhang Demei , Wang Rui , Han Shijian , Li Zhigang , Xiao Jiming , Li Yangrui , Wang Lingqiang , Li Suli
Published: August 2022

Sugarcane somatic cell hybridization can break through the barrier of genetic incompatibility between distantly related species in traditional breeding. However, the molecular mechanisms of sugarcane protoplast regeneration and the conditions for protoplast preparation remain largely unknown. In this study, young sugarcane (ROC22) leaves were enzymatically digested, and the viability of protoplasts reached more than 90% after enzymatic digestion (Enzymatic combination: 2% cellulase + 0.5% pectinase + 0.1% dissociative enzyme + 0.3% hemicellulase, pH = 5.8). Transcriptome sequencing was performed on young sugarcane leaves and protoplasts after enzymatic digestion to analyze the differences in gene expression in somatic cells before and after enzymatic digestion. A total of 117,411 unigenes and 43,460 differentially expressed genes were obtained, of which 21,123 were up-regulated and 22,337 down-regulated. The GO terms for the 43,460 differentially expressed genes (DEGs) were classified into three main categories: biological process, cellular component and molecular function, which related to developmental process, growth, cell proliferation, transcription regulator activity, signal transducer activity, antioxidant activity, oxidative stress, kinase activity, cell cycle, cell differentiation, plant hormone signal transduction, and so on. After enzymatic digestion of young sugarcane leaves, the expressions of GAUT, CESA, PSK, CyclinB, CyclinA, CyclinD3 and cdc2 genes associated with plant regeneration were significantly down-regulated to 65%, 47%, 2%, 18.60%, 21.32%, 52% and 45% of young leaves, respectively. After enzymatic digestion, Aux/IAA expression was up-regulated compared with young leaves, and Aux/IAA expression was 3.53 times higher than that of young leaves. Compared with young leaves, these key genes were significantly changed after enzymatic digestion. These results indicate that the process of somatic enzymatic digestion process may affect the regeneration of heterozygous cells to a certain extent.
Alcohol Induces Zebrafish Skeletal Muscle Atrophy through HMGB1/TLR4/NF-κB Signaling
Authors: Wen Wei , Sun Chenchen , Chen Zhanglin , Yang Dong , Zhou Zuoqiong , Peng Xiyang , Tang Changfa
Published: August 2022

Excessive alcohol consumption can cause alcoholic myopathy, but the molecular mechanism is still unclear. In this study, zebrafish were exposed to 0.5% alcohol for eight weeks to investigate the effect of alcohol on skeletal muscle and its molecular mechanism. The results showed that the body length, body weight, cross-sectional area of the skeletal muscle fibers, Ucrit, and MO2max of the zebrafish were significantly decreased after alcohol exposure. The expression of markers of skeletal muscle atrophy and autophagy was increased, and the expression of P62 was significantly reduced. The content of ROS, the mRNA expression of sod1 and sod2, and the protein expression of Nox2 were significantly increased. In addition, we found that the inflammatory factors Il1β and Tnfα were significantly enriched in skeletal muscle, and the expression of the HMGB1/TLR4/NF-κB signaling axis was also significantly increased. In summary, in this study, we established a zebrafish model of alcohol-induced skeletal muscle atrophy and further elucidated its pathogenesis.
Retro-Malleolar Z-Plasty of Flexor Hallucis Longus Tendon in Post-Traumatic Checkrein Deformity: A Case Series and Literature Review
Authors: Polichetti Chiara , Greco Tommaso , Inverso Michele , Maccauro Giulio , Forconi Fabrizio , Perisano Carlo
Published: August 2022

Checkrein deformity (CD) is a dynamic deformity of the hallux characterized by flexion contracture of the interphalangeal (IF) joint and extension contracture of the metatarsophalangeal (MTP) joint, worsened by ankle dorsiflexion. It is due to post-traumatic or ischemic retraction of the long hallux flexor tendon (FHL) following soft tissue trauma, leg fractures, ankle fractures and, more rarely, calcaneal or talar fractures. Diagnosis is essentially clinical, associated with imaging, to rule out unrecognized causes and evaluate fracture healing process. Few cases are reported in literature without univocal treatment. Background and Objectives: To analyze clinical and functional outcomes in patients with CD treated with release and retro-malleolar Z-plasty lengthening of FHL tendon. Materials and Methods: Patients diagnosed with CD treated with retro-malleolar (at tarsal tunnel) Z-plasty lengthening of the FHL tendon between January 2016 and August 2020 were included. Clinical and functional outcomes were collected on admission and post-surgery and analysed retrospectively. Patients with a minimum follow-up of 18 months were included. Results: A total of 14 patients, with mean age of 37.4 years old, with CD diagnosis were included in the study. All patients were suffering from post-traumatic CD and the mean time from trauma to onset of deformity was of 7 months (range 1–12). At a mean follow-up of 31.8 months (range 18–48) we found a significant improvement (p < 0.05) in terms of pain relief (VAS), function (AOFAS score) and ROM of the IP and MTP hallux joints. No recurrence, loss of strength, nerve injury or tarsal tunnel syndrome were observed. No patient required revision surgery. Conclusions: In this case series the retro-malleolar FHL tendon Z-plasty proved to be a suitable option for CD correction, allowing a good clinical and functional recovery.
Surgical Reconstruction with Tendon Allografting Following Iatrogenic Rupture of the Plantar Fascia: A Case Report
Authors: Chin Chien-Ming , Tang Huan-Ming , Yang Kai-Chiang , Chen Ing-Ho , Wang Chen-Chie
Published: August 2022

Plantar fasciitis is a common cause of heel pain, and the disorder is generally self-limiting after adequate conservative treatment. When conservative treatment is unsuccessful, surgical release is an effective treatment option. Here we report a case of iatrogenic plantar fascia rupture after surgical release for treatment of recalcitrant plantar fasciitis. Preoperative MRI revealed a 4.2 cm gap between the distal fascia stump and the calcaneal tuberosity in the sagittal view at 8 months post-injury. To circumvent the possibility of rupture site retear or poor tissue healing by direct repair, we used tendon allografting for the reconstruction of the chronic plantar fascia rupture. The patient gradually recovered after the surgery. Complications of plantar fascia rupture after surgical release is a potential risk but rarely observed. Chronic plantar fascia rupture with medial arch collapse is difficult to treat. We used a tendon allograft to reconstruct the plantar fascia, restoring its function and mechanical strength. After 5 years of follow-up, no complications were reported, and magnetic resonance imaging indicated the reconstructed plantar fascia tissue to be in good condition.
Assessment of Tocilizumab (Humanized Monoclonal Antibody) for Therapeutic Efficacy and Clinical Safety in Patients with Coronavirus Disease (COVID-19)
Authors: Ullah Sami , Abid Radhya , Haider Sana , Khuda Fazli , Albadrani Ghadeer M. , Abdulhakim Jawaher A. , Altyar Ahmed E. , Abdel-Daim Mohamed M. , Halimi Syed Muhammad Ashhad , Khalil Atif Ali Khan
Published: August 2022

Background and objectives: COVID-19 patients exhibit a broad range of manifestations, presenting with a flu-like respiratory tract infection that can advance to a systemic and severe disease characterized by pneumonia, pulmonary edema, severe damage to the airways, and acute respiratory distress syndrome (ARDS, causing fatality in 70% of COVID-19 cases). A ‘cytokine storm’ profile is found in most severely influenced COVID-19 patients. The treatment protocol of the disease also includes tocilizumab, which is a humanized monoclonal antibody used to treat autoimmune and inflammatory conditions. This study was designed (1) to assess the role of tocilizumab in COVID-19 patients regarding therapeutic efficacy through evaluation of cytokine release syndrome (CRS) resolution and anticoagulant effect, analyzing clinical safety via monitoring of associated adverse effects profile; and (2) to compare the clinical safety and therapeutic efficacy of institutional treatment regimen (alone) versus tocilizumab added to an institutional treatment module in COVID-19 patients. Materials and Methods: In this study, the endpoints parametric assessment of severely diseased patients of COVID-19 was performed (total n = 172, control group (institutional protocol treatment provided), n = 101 and test group (tocilizumab provided), n = 71) at the Khyber Teaching Institution, MTI, Peshawar. The assessments were compared using non-parametric analyses at baseline and after a follow-up of 12–18 days until the patient discharged or expired. Results: Results of the study revealed an insignificant difference among the control vs. test group in resolving inflammatory parameters (C-reactive protein (CRP) 21.30 vs. 50.07; p = 0.470, ferritin 482.9 vs. 211.5; p = 0.612, lactate dehydrogenase (LDH) 29.12 vs.18.8; p = 0.0863, and D-dimer 464 vs.164.4; p = 0.131). However, a statistically significant difference was found between the control group and test group regarding coagulation parameters (international normalized ratio (INR) 0.12 vs. −0.07; p ≤ 0.001; activated partial thromboplastin time (aPTT) 0.42 vs. −1.16; p ≤ 0.001; prothrombin time (PT) 0.31 vs. −0.96; p ≤ 0.001; platelet count −12.34 vs. −1.47; p = 0.012) and clinical survival rate (89.10 vs. 90.14; p < 0.001). Furthermore, there was significantly higher infection rates and raised alanine aminotransferase (ALT) and alkaline phosphatase (ALP) associated with the tocilizumab group as compared to those receiving institutional treatment (bacterial infections: 0.99% vs. 15.49%; p ≤ 0.01, ALT: 3.96% vs. 28.16%; p ≤ 0.01, ALP: 1.98% vs. 22.53%; p ≤ 0.01). Conclusions: From this study, it was concluded that tocilizumab can be a better drug of choice in terms of efficacy, particularly in resolving coagulopathy in severe COVID-19 patients.
Effect of Direct-Acting Antiviral Agents on Gastroesophageal Varices in Patients with Hepatitis C Virus-Related Cirrhosis
Authors: Hisanaga Hiroshi , Takedatsu Hidetoshi , Emori Keigo , Inoue Hiroto , Kunitake Yasuhumi , Nakane Tomoyuki , Fukunaga Shuhei , Ide Tatsuya , Mitsuyama Keiichi , Torimura Takuji
Published: August 2022

Aim: In patients with hepatitis C virus-related liver cirrhosis (LC) who achieve sustained virological responses (SVRs) through treatment with direct-acting antiviral agents (DAAs), it remains unclear whether there are improvements in gastroesophageal varices (GEVs) and portal hypertension. We investigated changes in liver function and GEVs that occurred after DAA therapy. Materials and Methods: We evaluated the medical records of 195 patients with hepatitis C virus-related LC who received DAAs. A total of 171 patients achieved SVRs, among whom 36 had GEVs before or after receiving DAA therapy. The liver function, fibrosis, and GEVs were re-evaluated every 6 months after receiving DAA therapy. The risk factors for progressive GEVs were investigated. Results: DAA therapy resulted in improvements in liver function (indicated by aspartate transaminase, alanine transaminase, and serum albumin levels) and fibrosis (indicated by type IV collagen levels and the Fibrosis-4 index). After receiving DAA therapy, 27 patients had stable GEVs and 9 had progressive GEVs. With respect to GEV grades before DAA therapy, there was a significant difference between patients with stable and progressive GEVs (p = 0.027). Presence of grade-2 GEVs before starting DAA therapy was a risk factor for GEV progression (odds ratio: 5.83; p = 0.04). Patients with grade-2 GEVs had significantly shorter progression-free periods than those with grade < 2 GEVs (p = 0.025). Conclusions: DAA therapy does not ameliorate GEVs. Furthermore, grade-2 GEVs can worsen after DAA therapy. Therefore, patients with GEVs of grades ≥ 2 should undergo endoscopic surveillance after receiving DAAs.
Anterior-Posterior and Inter-Limb Lower Body Strength Asymmetry in Soccer, Basketball, Futsal, and Volleyball Players
Authors: Parpa Koulla , Michaelides Marcos
Published: August 2022

Background and Objectives: The purpose of this study was twofold: (1) to examine strength asymmetries in elite athletes of different sports and (2) to examine the magnitude of asymmetries between elite and u18 athletes. Materials and Methods: A total of 254 athletes participated in this study. For the first purpose of the study, the group consisted of adult professional male basketball players (n = 30), elite male (n = 30) and female soccer players (n = 20), male futsal players (n = 30), elite male goalkeepers (n = 22), and professional female volleyball players (n = 20). For the second purpose, male youth goalkeepers (n = 22), youth female (n = 20) and male soccer players (n = 30), and youth male basketball players (n = 30) were compared to the adult athletes of the same sport. Asymmetries were measured utilizing a Humac Norm and Rehabilitation device. Testing included three maximal concentric flexion and extension repetitions at an angular speed of 60°/s. The differences in asymmetries were assessed using ANOVA followed by an LS means post-hoc analysis. An independent samples t-test was used to identify the differences between adult and youth players. Results: It was indicated that none of the groups demonstrated asymmetries greater than 10%, other than the elite female soccer players and female volleyball players. Significant differences were demonstrated between youth and adult soccer players (males and females), with the adult groups demonstrating increased asymmetries. Conclusions: Special consideration should be given to female soccer players and volleyball players, as soccer and volleyball practice and competition at the professional level may induce greater lower -limb asymmetries. The isokinetic parameters can be useful for planning strength and conditioning interventions in order to reduce or prevent those imbalances. Additional unilateral and bilateral jumping testing is encouraged for the verification of imbalances.
Development and Evaluation of an Antimicrobial Formulation Containing Rosmarinus officinalis
Authors: Macedo Lucas Malvezzi de , Santos Érica Mendes dos , Ataide Janaína Artem , Silva Gabriela Trindade de Souza e , Guarnieri João Paulo de Oliveira , Lancellotti Marcelo , Jozala Angela Faustino , Rosa Paulo Cesar Pires , Mazzola Priscila Gava
Published: August 2022

Rosmarinus officinalis belongs to the Lamiaceae family, and its constituents show antioxidant, anti-inflammatory, antidepressant, antinociceptive, and antibacterial properties. The aim of this study was to develop a topical formulation with R. officinalis extract that had antimicrobial and antioxidant activity. Maceration, infusion, Soxhlet, and ultrasound were used to produce rosemary extracts, which were submitted to antioxidant, compound quantification, cell viability, and antimicrobial assays. Infusion and Soxhlet showed better results in the DPPH assay. During compound quantification, infusion showed promising metabolite extraction in phenolic compounds and tannins, although maceration was able to extract more flavonoids. The infusion and ultrasound extracts affected more strains of skin bacteria in the disk diffusion assays. In the minimum inhibitory concentration assay, the infusion extract showed results against S. aureus, S. oralis, and P. aeruginosa, while ultrasound showed effects against those three bacteria and E. coli. The infusion extract was chosen to be incorporated into a green emulsion. The infusion extract promoted lower spreadability and appropriated the texture, and the blank formulation showed high levels of acceptance among the volunteers. According to the results, the rosemary extract showed promising antioxidant and antimicrobial activity, and the developed formulations containing this extract were stable for over 90 days and had acceptable characteristics, suggesting its potential use as a phytocosmetic. This paper reports the first attempt to produce an oil-in-water emulsion using only natural excipients and rosemary extract, which is a promising novelty, as similar products cannot be found on the market or in the scientific literature.
Seed Phytochemical Profiling of Three Olive Cultivars, Antioxidant Capacity, Enzymatic Inhibition, and Effects on Human Neuroblastoma Cells (SH-SY5Y)
Authors: Gouvinhas Irene , Garcia Juliana , Granato Daniel , Barros Ana
Published: August 2022

This work evaluated the phytochemical composition of olive seed extracts from different cultivars (‘Cobrançosa’, ‘Galega’, and ’Picual’) and their antioxidant capacity. In addition, it also appraised their potential antineurodegenerative properties on the basis of their ability to inhibit enzymes associated with neurodegenerative diseases: acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase (TYR). To achieve this goal, the phenolic composition of the extracts was determined through high-performance liquid chromatography coupled with photodiode-array detection and electrospray ionization/ion trap mass spectrometry (HPLC-DAD-ESI/MSn). The antioxidant capacity was assessed by two different methods (ABTS•+ and DPPH•), and the antineurodegenerative potential by the capacity of these extracts to inhibit the aforementioned related enzymes. The results showed that seed extracts presented a high content of phenolic compounds and a remarkable ability to scavenge ABTS•+ and DPPH•. Tyrosol, rutin, luteolin-7-glucoside, nüzhenide, oleuropein, and ligstroside were the main phenolic compounds identified in the extracts. ‘Galega’ was the most promising cultivar due to its high concentration of phenolic compounds, high antioxidant capacity, and remarkable inhibition of AChE, BChE, and TYR. It can be concluded that olive seed extracts may provide a sustainable source of bioactive compounds for medical and industrial applications.
Heterobimetallic Ru(II)/M (M = Ag+, Cu2+, Pb2+) Complexes as Photosensitizers for Room-Temperature Gas Sensing
Authors: Nasriddinov Abulkosim , Tokarev Sergey , Platonov Vadim , Botezzatu Anatoly , Fedorova Olga , Rumyantseva Marina , Fedorov Yuri
Published: August 2022

This work is devoted to the investigation of heterobimetallic Ru(II) complexes as photosensitizers for room-temperature photoactivated In2O3-based gas sensors. Nanocrystalline In2O3 was synthesized by the chemical precipitation method. The obtained In2O3 matrix has a single-phase bixbyite structure with an average grain size of 13–14 nm and a specific surface area of 72 ± 3 m2/g. The synthesis of new ditope ligands with different coordination centers, their ruthenium complexes, and the preparation of heterobimetallic complexes with various cations of heavy and transition metals (Ag+, Pb2+, or Cu2+) is reported. The heterobimetallic Ru(II) complexes were deposited onto the surface of the In2O3 matrix by impregnation. The obtained hybrid materials were characterized by X-ray fluorescent analysis, FTIR spectroscopy, and optical absorption spectroscopy. The elemental distribution on the hybrids was characterized by energy-dispersive X-ray spectroscopy (EDS) mapping. The gas sensor properties were investigated toward NO2, NO, and NH3 at room temperature under periodic blue LED irradiation. It was identified that the nature of the second binding cation in Ru(II) heterobimetallic complexes can influence the selectivity toward different gases. Thus, the maximum sensor signal for oxidizing gases (NO2, NO) was obtained for hybrids containing Ag+ or Pb2+ cations while the presence of Cu2+ cation results in the highest and reversible sensor response toward ammonia. This may be due to the specific adsorption of NH3 molecules on Cu2+ cations. On the other hand, Cu2+ ions are proposed to be active sites for the reduction of nitrogen oxides to N2. This fact leads to a significant decrease in the sensor response toward NO2 and NO gases.
A Multiple-Stimuli-Responsive Amphiphilic Copolymer for Antifouling and Antibacterial Functionality via a “Resistance–Kill–Release” Mechanism
Authors: Liao Xiaohan , Niu Kai , Liu Feng , Zhang Yongming
Published: August 2022

In recent years, polymers with stimuli-responsive properties have been increasingly reported on due to their diverse applications. However, most of the studies have only focused on the performance of polymers under specific scenarios. The laws of changes in the properties in response to various external stimuli have been less systematically and quantitatively studied. In this paper, we prepared an amphiphilic polymer (PadaMX and PAdaM3QA−X) with temperature-, pH-, ion-, and β-cyclodextrin (β-CD)-responsive properties. According to the cloud point tested by the UV-Vis method, the lower critical soluble temperature (LCST) of PAdaM3QA−10% was more sensitive to a change in pH and less sensitive to a change in ions compared with PadaM3 due to quaternized side chains with a stronger intramolecular mutual repulsion. We then fabricated the coatings with responsive properties by immobilizing the adamantyl groups on β-CD-modified surfaces. The hydrophilicity of the coatings was improved after quaternization, as proven by the water contact angle (WCA) measurement. The antifouling and antibacterial performance was further evaluated via the fluorescence intensity of bovine serum albumin (BSA) adsorbed on the surfaces and the spread plate method. A 78.4% BSA desorption rate and a 96.8% sterilization rate were achieved by the PAdaM3QA−10% coating. In summary, this work prepared a multiple-stimuli-responsive amphiphilic copolymer for antifouling and antibacterial functionality via a “resistance–kill–release” mechanism.
Evidence of Isomerization in the Michael-Type Thiol-Maleimide Addition: Click Reaction between L-Cysteine and 6-Maleimidehexanoic Acid
Authors: Niño-Ramírez Víctor Alfonso , Insuasty-Cepeda Diego Sebastián , Rivera-Monroy Zuly Jenny , Maldonado Mauricio
Published: August 2022

The reaction between L-cysteine (Cys) and 6-maleimidohexanoic acid (Mhx) in an aqueous medium at different levels of pH was analyzed via RP-HPLC, finding the presence of two reaction products throughout the evaluated pH range. By means of solid-phase extraction (SPE), it was possible to separate the products and obtain isolated profiles enriched up to 80%. The products were analyzed individually through mass spectrometry, DAD-HPLC, NMR 1H, 13C, and two-dimensional evidence of isomerization between the hydrogen atoms of the α-amino and the thiol group present in the cysteine. Thus, it was concluded that the products obtained corresponded to a mixture of the isomer Cys-S-Mhx, where the adduct is formed by a thioether bond, and the isomer Cys-NH-Mhx, in which the union is driven by the amino group. We consider that the phenomenon of isomerization is an important finding, since it has not previously been reported for this reaction.
Novel Isoxazole Derivative Attenuates Ethanol-Induced Gastric Mucosal Injury through Inhibition of H+/K+-ATPase Pump, Oxidative Stress and Inflammatory Pathways
Authors: Razzaq Sidra , Minhas Amber Mahmood , Qazi Neelum Gul , Nadeem Humaira , Khan Arif-ullah , Ali Fawad , Hassan Syed Shams ul , Bungau Simona
Published: August 2022

Isoxazole derivatives are significant enough due to their wide range of pharmacological and therapeutic activities. The purpose of the current study is to use computational, in vitro, in vivo, and extensive molecular approaches to examine the possible anti-ulcer activity of 4-benzylidene-3 methyl-1,2-isoxazol-5(4H)-one (MBO). Biovia Discovery Studio visualizer (DSV) was utilized for virtual screening. A tissue antioxidant investigation, H+/K+-ATPase test, and anti-H. pylori activities were carried out. ELISA, immunohistochemistry, and PCR methods were employed for the proteome analysis. An ethanol-induced stomach ulcer model was used to examine the anti-ulcer potential in rats. The binding affinities for MBO ranged from −5.4 to −8.2 Kcal/mol. In vitro findings revealed inhibitory activity against H. pylori and the H+/K+-ATPase pump. It also enhanced levels of glutathione, catalase, and glutathione-S-transferase and reduced lipid peroxidation levels in gastric tissues of rats. In vivo results showed the gastro-protective effect of MBO (30 mg/kg) in ulcerative rat stomachs. The proteomic study revealed decreased expression of inflammatory markers (cyclooxygenase-2, p-NFkB, and TNF-α). In RT-PCR analysis, the expression levels of H+/K+-ATPase were reduced. Furthermore, ADMET (absorption, distribution, metabolism, excretion and toxicity) studies revealed that MBO has high GIT solubility and has a safer profile for cardiac toxicity. This study suggests that MBO displayed anti-ulcer potential, which may have been mediated through the inhibition of the H+/K+-ATPase pump, as well as antioxidant and anti-inflammatory pathways. It has the potential to be a lead molecule in the treatment of peptic ulcers with fewer adverse effects.
Aβ and Tau Interact with Metal Ions, Lipid Membranes and Peptide-Based Amyloid Inhibitors: Are These Common Features Relevant in Alzheimer’s Disease?
Authors: Di Natale Giuseppe , Sabatino Giuseppina , Sciacca Michele Francesco Maria , Tosto Rita , Milardi Danilo , Pappalardo Giuseppe
Published: August 2022

In the last two decades, the amyloid hypothesis, i.e., the abnormal accumulation of toxic Aβ assemblies in the brain, has been considered the mainstream concept sustaining research in Alzheimer’s Disease (AD). However, the course of cognitive decline and AD development better correlates with tau accumulation rather than amyloid peptide deposition. Moreover, all clinical trials of amyloid-targeting drug candidates have been unsuccessful, implicitly suggesting that the amyloid hypothesis needs significant amendments. Accumulating evidence supports the existence of a series of potentially dangerous relationships between Aβ oligomeric species and tau protein in AD. However, the molecular determinants underlying pathogenic Aβ/tau cross interactions are not fully understood. Here, we discuss the common features of Aβ and tau molecules, with special emphasis on: (i) the critical role played by metal dyshomeostasis in promoting both Aβ and tau aggregation and oxidative stress, in AD; (ii) the effects of lipid membranes on Aβ and tau (co)-aggregation at the membrane interface; (iii) the potential of small peptide-based inhibitors of Aβ and tau misfolding as therapeutic tools in AD. Although the molecular mechanism underlying the direct Aβ/tau interaction remains largely unknown, the arguments discussed in this review may help reinforcing the current view of a synergistic Aβ/tau molecular crosstalk in AD and stimulate further research to mechanism elucidation and next-generation AD therapeutics.
Sample Preparation and Analytical Methods for Identifying Organic Compounds in Bituminous Emissions
Authors: Deller Zachary , Maniam Subashani , Giustozzi Filippo
Published: August 2022

Bitumen is a major construction material that can emit harmful fumes when heated. These fumes pose health risks to workers and communities near construction projects or asphalt mixing plants. The chemical complexity of bitumen fumes and the increasing use of additives add to the difficulty of analytically quantifying the harmful chemicals emitted using a single technique. Research on bitumen emissions consists of numerous sample preparation and analytical methods. There are a range of considerations to be made when deciding on an appropriate sample preparation method and instrumental configuration to optimise the analysis of specific organic contaminants in emissions. Researchers investigating emissions from bituminous materials may need to consider a range of analytical techniques to quantify harmful chemicals and assess the efficacy of new additives. This review summarises the primary methodologies for sample preparation and analytical techniques used in bitumen research and discusses future challenges and solutions.
Restoration of Hepatic and Intestinal Integrity by Phyllanthus amarus Is Dependent on Bax/Caspase 3 Modulation in Intestinal Ischemia-/Reperfusion-Induced Injury
Authors: Afolabi Ayobami Oladele , Akhigbe Tunmise Maryanne , Odetayo Adeyemi Fatai , Anyogu Davinson Chuka , Hamed Moses Agbomhere , Akhigbe Roland Eghoghosoa
Published: August 2022

Ethnopharmacological relevance: Oxidative stress is a key player in intestinal ischemia/reperfusion (I/R) injury (IIRI) with a tendency to trigger systemic inflammatory response, resulting in progressive distal organ injury. To date, the role of Bax/caspase 3 signaling in IIRI has not been reported. Furthermore, the discovery of a safe and effective drug remains pertinent in improving the outcome of IIRI. Therefore, this study investigated the role of Bax/caspase 3 signaling in intestinal I/R-induced intestinal and hepatic injury. In addition, the protective effect and possible associated mechanism of action of methanolic Phyllanthus amarus leaf extract (PA) against intestinal I/R-induced intestinal and hepatic injury were evaluated. Materials and methods: Fifty male Wistar rats were randomized into five groups (n = 10). The sham-operated group was received 0.5 mL of distilled water for seven days prior to the sham surgery, while the IIRI, febuxostat (FEB) + IIRI, low-dose PA (LDPA) + IIRI, and high-dose PA (HDPA) + IIRI groups underwent the I/R procedure. In addition to the procedure, IIRI, FEB + IIRI, LDPA + IIRI, and HDPA + IIRI received 0.5 mL of distilled water, 10 mg/kg of febuxostat, 200 mg/kg of PA, and 400 mg/kg of PA, respectively, for seven days prior to the I/R procedure. Results: Administration of methanolic Phyllanthus amarus leaf extracts attenuated the intestinal I/R-induced rise in intestinal and hepatic injury markers, malondialdehyde, nitric oxide, TNF-α, IL-6, and myeloperoxidase activities. In addition, Phyllanthus amarus ameliorated I/R-induced suppression of reduced glutathione, thiol and non-thiol proteins, and superoxide dismutase, catalase, and glutathione peroxidase activities in intestinal and hepatic tissues. These were coupled with the suppression of I/R-induced bacterial translocation, downregulation of I/R-induced activation of Bax/caspase 3 signaling, and improvement of I/R-induced distortion of intestinal and hepatic histoarchitecture by Phyllanthus amarus. Conclusion: Methanolic Phyllanthus amarus leaf extract protects against intestinal and hepatic injuries associated with intestinal I/R by suppressing oxidative-stress-mediated activation of Bax/caspase 3 signaling. The beneficial effects of Phyllanthus amarus may be ascribed to its constituent bioactive molecules, especially tannins, anthocyanin, alkaloids, and phenolics.
Baicalin Alleviate Apoptosis via PKC-MAPK Pathway in Porcine Peritoneal Mesothelial Cells Induced by Glaesserella parasuis
Authors: Lu Qirong , Zhou Lang , Wang Ziyue , Li Xiaomin , Ding Li , Qiu Yinsheng , Guo Pu , Ye Chun , Fu Shulin , Wu Zhongyuan , Liu Yu
Published: August 2022

Glaesserella parasuis (GPS), a causative agent of Glässer’s disease, is thought to be the main fatal cause of peritonitis in swine, thus resulting in high mortality and morbidity and significant economic losses to the swine industry. However, the mechanisms of GPS infection-induced apoptosis and possible therapeutic pathway for GPS infection in peritonitis remain unclear. Baicalin has important biological functions during disease treatment, such as antiviral, bacterial inhibition, anti-apoptosis, and anti-inflammatory. However, whether baicalin has anti-apoptotic effects during the process of GPS infection in peritonitis is unclear. In the present study, the anti-apoptotic effect and mechanisms of baicalin in GPS infection-induced apoptosis were investigated in porcine peritoneal mesothelial cells (PPMC). The results showed that baicalin could inhibit the apoptosis rate occurrence of PPMC induced by GPS to various degrees and inhibit the expression of apoptosis-related genes and cleaved caspase-3. Meanwhile, baicalin significantly antagonized the expression of p-JNK, p-p38, and p-ERK induced by GPS in PPMC. These findings for the first time demonstrate that baicalin exerted the effect of antagonizing GPS induced apoptosis in PPMC by inhibiting the activation of the PKC-MAPK pathway and could be a therapeutic option in the management of GPS infection.
Supported Eosin Y as a Photocatalyst for C-H Arylation of Furan in Batch and Flow
Authors: Rossi Sergio , Herbrik Fabian , Resta Simonetta , Puglisi Alessandra
Published: August 2022

Eosin Y is one of the most popular organic dyes used as a photoredox catalyst and is largely employed in photochemical reactions both as a homogeneous and heterogeneous photocatalyst after immobilization. Immobilization of Eosin Y onto a solid support has many advantages, such as the possibility of recovery and reuse of the photocatalyst and the possibility of its use under flow conditions. In this paper, we report our findings on the immobilization of Eosin Y onto Merrifield resin and its application in the direct photochemical arylation of furan with aryldiazonium salts. The synthesized supported photocatalyst was used in batch reactions under heterogeneous conditions with different aryl diazonium salts, and its recovery and recycle were demonstrated for up to three times. The immobilized photocatalyst was then loaded in a packed-bed reactor and used under continuous flow conditions. The flow reaction allowed the arylated products to be obtained with higher productivity and space-time-yield than the batch in a very short reaction time.
Liquid Biopsy and Circulating Biomarkers for the Diagnosis of Precancerous and Cancerous Oral Lesions
Authors: Gattuso Giuseppe , Crimi Salvatore , Lavoro Alessandro , Rizzo Roberta , Musumarra Giorgia , Gallo Simona , Facciponte Flavia , Paratore Sabrina , Russo Angela , Bordonaro Roberto , Isola Gaetano , Bianchi Alberto , Libra Massimo , Falzone Luca
Published: August 2022

Oral cancer is one of the most common malignancies worldwide, accounting for 2% of all cases annually and 1.8% of all cancer deaths. To date, tissue biopsy and histopathological analyses are the gold standard methods for the diagnosis of oral cancers. However, oral cancer is generally diagnosed at advanced stages with a consequent poor 5-year survival (~50%) due to limited screening programs and inefficient physical examination strategies. To address these limitations, liquid biopsy is recently emerging as a novel minimally invasive tool for the early identification of tumors as well as for the evaluation of tumor heterogeneity and prognosis of patients. Several studies have demonstrated that liquid biopsy in oral cancer could be useful for the detection of circulating biomarkers including circulating tumor DNA (ctDNA), microRNAs (miRNAs), proteins, and exosomes, thus improving diagnostic strategies and paving the way to personalized medicine. However, the application of liquid biopsy in oral cancer is still limited and further studies are needed to better clarify its clinical impact. The present manuscript aims to provide an updated overview of the potential use of liquid biopsy as an additional tool for the management of oral lesions by describing the available methodologies and the most promising biomarkers.
Neuroplasticity as a Foundation for Decision-Making in Space
Authors: Rappaport Margaret Boone , Corbally Christopher J.
Published: August 2022

This is an exploratory review of two very recent, intersecting segments of space science: neuroplasticity in space, and decision-making in space. The high level of neuroplasticity in humans leads to unfortunate neurological and physical deconditioning while the body adjusts to the new space environment. However, neuroplasticity may also allow recovery and continued functioning of decision-making at a level necessary for mission completion. Cosmic radiation, microgravity, heightened levels of carbon dioxide in spacecraft, and other factors are being explored as root causes of neurological and physical deconditioning in space. The goal of this paper is to explore some of the lines of causation that show how these factors affect the capacity of humans to make decisions in space. Either alone or in groups, it remains essential that humans retain an ability to make decisions that will save lives, protect equipment, complete missions, and return safely to Earth. A final section addresses healthcare, medical intervention, and remediation that could help to “harness” neuroplasticity before, during, and after spaceflight. The dual nature of human neuroplasticity renders it both a cause of problems and also potentially the foundation of remediation. The future of research on both neuroplasticity and human decision-making promises to be full of surprises, both welcome and otherwise. It is an exciting time in research on space medicine.
Diet-Derived Circulating Antioxidants and Risk of Digestive System Tumors: A Mendelian Randomization Study
Authors: Yin Linlin , Yan Haihao , Chen Kangdi , Ji Zuhong , Zhang Xiuhua , Ji Guozhong , Zhang Bin
Published: August 2022

Previous observational case-control studies have shown significant controversy over the impact of dietary intake-related circulating antioxidants on the risk of digestive system tumors. We conducted a two-sample Mendelian randomized (MR) analysis to determine whether there was a significant causal relationship between increased levels of circulating antioxidants and digestive system tumors. Our circulating antioxidants (vitamin C, carotenoids, vitamin A, and vitamin E) were derived from absolute circulating antioxidants and circulating antioxidant metabolites, and their corresponding instrumental variables were screened from published studies. The digestive system tumors we studied included colorectal, gastric, pancreatic, liver, and esophageal cancer, and the corresponding summary GAWS (genome-wide association study) data were obtained from the UK Biobank database. We first evaluated the causal relationship between each tumor and circulating antioxidants and then used meta-analysis to summarize the results of MR analysis of different tumors. No significant associations were noted for genetically predicted circulating antioxidants and higher risk of digestive system tumors in our study. The pooled ORs (odds ratio) are 0.72 (95% CI: 0.46–1.11; β-carotene), 0.93 (95% CI: 0.81–1.08; lycopene), 2.12 (95% CI: 0.31–14.66; retinol), and 0.99 (95% CI: 0.96–1.02; ascorbate) for absolute circulating antioxidants; for circulating antioxidant metabolites, the pooled ORs for digestive system tumors risk per unit increase of antioxidants were 1.29 (95% CI: 0.39–4.28; α-tocopherol), 1.72 (95% CI: 0.85–3.49; γ-tocopherol), 1.05 (95% CI: 0.96–1.14; retinol), and 1.21 (95% CI: 0.97–1.51; ascorbate), respectively. Our study suggested that increased levels of dietary-derived circulating antioxidants did not reduce the risk of digestive system tumors.
Local Ecological Knowledge Reveals Change in Seagrass Social–Ecological Systems
Authors: Jones Benjamin L. H. , Unsworth Richard K. F. , Nordlund Lina M. , Ambo-Rappe Rohani , La Nafie Yayu A. , Lopez Mary Rose , Udagedara Susantha , Cullen-Unsworth Leanne C.
Published: August 2022

It is widely recognized that humanity is currently facing multiple planetary crises, including the widespread loss of biodiversity and a rapidly changing climate. The impacts of these crises are often far reaching and threaten food security (SDG goal two: zero hunger). Small-scale fisheries are estimated to provide livelihoods for over one hundred million people and sustenance for approximately one billion people but face a plethora of threats and challenges linked to planetary crises. In this multi-country assessment (150 coastal villages across five countries within the Indo-Pacific), household interviews revealed how seagrass meadows are important to small-scale fisheries, particularly as a place to find and collect a reliable source of food. Interviews also revealed that habitat loss and the over-exploitation of these resources are placing people and their food security at risk. This study exposed how dynamic local ecological knowledge can be, uncovering personal opinions and responsibilities that result in the hybridization of knowledge. Here, we demonstrate the importance of using local ecological knowledge to incorporate shared values into management but also highlight that an integrated approach, pairing local and conventional scientific knowledge, is needed urgently if we are to meet the needs of people while simultaneously conserving biodiversity.
Energy-Momentum Complex in Higher Order Curvature-Based Local Gravity
Authors: Capozziello Salvatore , Capriolo Maurizio , Lambiase Gaetano
Published: August 2022

An unambiguous definition of gravitational energy remains one of the unresolved issues of physics today. This problem is related to the non-localization of gravitational energy density. In General Relativity, there have been many proposals for defining the gravitational energy density, notably those proposed by Einstein, Tolman, Landau and Lifshitz, Papapetrou, Møller, and Weinberg. In this review, we firstly explored the energy–momentum complex in an nth order gravitational Lagrangian L=Lgμν,gμν,i1,gμν,i1i2,gμν,i1i2i3,⋯,gμν,i1i2i3⋯in and then in a gravitational Lagrangian as Lg=(R¯+a0R2+∑k=1pakR□kR)−g. Its gravitational part was obtained by invariance of gravitational action under infinitesimal rigid translations using Noether’s theorem. We also showed that this tensor, in general, is not a covariant object but only an affine object, that is, a pseudo-tensor. Therefore, the pseudo-tensor ταη becomes the one introduced by Einstein if we limit ourselves to General Relativity and its extended corrections have been explicitly indicated. The same method was used to derive the energy–momentum complex in fR gravity both in Palatini and metric approaches. Moreover, in the weak field approximation the pseudo-tensor ταη to lowest order in the metric perturbation h was calculated. As a practical application, the power per unit solid angle Ω emitted by a localized source carried by a gravitational wave in a direction x^ for a fixed wave number k under a suitable gauge was obtained, through the average value of the pseudo-tensor over a suitable spacetime domain and the local conservation of the pseudo-tensor. As a cosmological application, in a flat Friedmann–Lemaître–Robertson–Walker spacetime, the gravitational and matter energy density in f(R) gravity both in Palatini and metric formalism was proposed. The gravitational energy–momentum pseudo-tensor could be a useful tool to investigate further modes of gravitational radiation beyond two standard modes required by General Relativity and to deal with non-local theories of gravity involving □−k terms.
Production of the Polysaccharide Pullulan by Aureobasidium pullulans Cell Immobilization
Authors: West Thomas P.
Published: August 2022

This review examines the immobilization of A. pullulans cells for production of the fungal polysaccharide pullulan. Pullulan is a water-soluble gum that exists structurally as a glucan consisting primarily of maltotriose units, which has a variety of food, non-food and biomedical applications. Cells can be immobilized by carrier-binding or entrapment techniques. The number of studies utilizing carrier-binding as a method to immobilize A. pullulans cells appears to outnumber the investigations using cell entrapment. A variety of solid supports, including polyurethane foam, sponge, diatomaceous earth, ion-exchanger, zeolite and plastic composite, have been employed to immobilize pullulan-producing A. pullulans cells. The most effective solid support that was used to adsorb the fungal cells was polyurethane foam which produced polysaccharide after 18 cycles of use. To entrap pullulan-producing fungal cells, agents such as polyurethane foam, polyvinyl alcohol, calcium alginate, agar, agarose, carrageenan and chitosan were investigated. Polysaccharide production by cells entrapped in polyurethane foam, polyvinyl alcohol or calcium alginate was highest and the immobilized cells could be reutilized for several cycles. It was shown that the pullulan content of the polysaccharide synthesized by cells entrapped in calcium alginate beads was low, which limits the method’s usefulness for pullulan production. Further, many of the entrapped fungal cells synthesized polysaccharide with a low pullulan content. It was concluded that carrier-binding techniques may be more effective than entrapment techniques for A. pullulans cell immobilization, since carrier-binding is less likely to affect the pullulan content of the polysaccharide being synthesized.