bluuebunny/crossref_metadata_2025 huggingface dataset:
Modern approaches on stem cells and scaffolding technology for osteogenic differentiation and regeneration
Authors: Kirankumar Shivaani , Gurusamy Narasimman , Rajasingh Sheeja , Sigamani Vinoth , Vasanthan Jayavardini , Perales Selene G , Rajasingh Johnson
Published: October 2021

The process of bone repair has always been a natural mystery. Although bones do repair themselves, supplemental treatment is required for the initiation of the self-regeneration process. Predominantly, surgical procedures are employed for bone regeneration. Recently, cell-based therapy for bone regeneration has proven to be more effective than traditional methods, as it eliminates the immune risk and painful surgeries. In clinical trials, various stem cells, especially mesenchymal stem cells, have shown to be more efficient for the treatment of several bone-related diseases, such as non-union fracture, osteogenesis imperfecta, osteosarcoma, and osteoporosis. Furthermore, the stem cells grown in a suitable three-dimensional scaffold support were found to be more efficient for osteogenesis. It has been shown that the three-dimensional bioscaffolds support and simulate an in vivo environment, which helps in differentiation of stem cells into bone cells. Bone regeneration in patients with bone disorders can be improved through modification of stem cells with several osteogenic factors or using stem cells as carriers for osteogenic factors. In this review, we focused on the various types of stem cells and scaffolds that are being used for bone regeneration. In addition, the molecular mechanisms of various transcription factors, signaling pathways that support bone regeneration and the senescence of the stem cells, which limits bone regeneration, have been discussed.
Radiography and Clinical Decision-Making in Chiropractic
Authors: Lopes Mark A. , Coleman Roger R. , Cremata Edward J.
Published: October 2021

The concern over x-ray exposure risks can overshadow the potential benefit of radiography, especially in cases where manual therapy is employed. Spinal malalignment cannot be accurately visualized without imaging. Manual therapy and the load tolerances of injured spinal tissues raise different criteria for the use of x-rays for spinal disorders than in medical practice. Current regulatory bodies rely on radiography risk assessments based on Linear-No-Threshold (LNT) risk models. There is a need to consider radiography guidelines for chiropractic which are different from those for medical practice. Radiography practice guidelines are summaries dominated by frequentist interpretations in the analysis of data from studies. In contrast, clinicians often employ a pseudo-Bayesian form of reasoning during the clinical decision-making process. The overrepresentation of frequentist perspectives in evidence-based practice guidelines alter decision-making away from practical assessment of a patient’s needs, toward an overly cautious standard applied to patients without regard to their risk/benefit likelihoods relating to radiography. Guidelines for radiography in chiropractic to fully assess the condition of the spine and spinal alignment prior to manual therapy, especially with high velocity, low amplitude spinal manipulation (HVLA-SM), should necessarily differ from those used in medical practice.
Successful management of bilateral internal carotid artery fusiform giant aneurysms in a patient with intimal fibroplasia using multiple flow diverters: With long-term imaging follow up
Authors: Roh Jieun , Baik Seung Kug , Yeom Jeong A , Na Joo-Young , Lee Sang-Won
Published: October 2021

The authors report a rare case of sequentially developed bilateral internal carotid artery (ICA) fusiform giant aneurysms in a patient with pathologically confirmed intimal fibroplasia. Both ICA fusiform aneurysms were treated with multiple flow diverter insertion and were well-managed over the past 5.5 years of follow-up. The development of aneurysms in this rare disease entity appears to be a lifelong process based on the authors’ observations in serial angiographic follow-up studies. Reconstruction therapy using flow-diverting stents in this unique condition may be a safe and effective treatment modality.
A Levenberg-Marquardt backpropagation method for unsteady squeezing flow of heat and mass transfer behaviour between parallel plates
Authors: Bilal Hazrat , Ullah Hakeem , Fiza Mehreen , Islam Saeed , Raja Muhammad Asif Zahoor , Shoaib Muhammad , Khan Ilyas
Published: October 2021

In this study, a new computing model by developing the strength of feed-forward neural networks with Levenberg-Marquardt Method (NN-BLMM) based backpropagation is used to find the solution of nonlinear system obtained from the governing equations of unsteady squeezing flow of Heat and Mass transfer behaviour between parallel plates. The governing partial differential equations (PDEs) for unsteady squeezing flow of Heat and Mass transfer of viscous fluid are converting into ordinary differential equations (ODEs) with the help of a similarity transformation. A dataset for the proposed NN-BLMM is generated for different scenarios of the proposed model by variation of various embedding parameters squeeze Sq, Prandtl number Pr, Eckert number Ec, Schmidt number Sc and chemical-reaction-parameter [Formula: see text]. Physical interpretation to various embedding parameters is assigned through graphs for squeeze Sq, Prandtl Pr, Eckert Ec, Schmidt Sc and chemical-reaction-parameter [Formula: see text]. The processing of NN-BLMM training (T.R), Testing (T.S) and validation (V.L) is employed for various scenarios to compare the solutions with the reference results. For the fluidic system convergence analysis based on mean square error (MSE), error histogram (E.H) and regression (R.G) plots is considered for the proposed computing infrastructures performance in term of NN-BLMM. The results based on proposed and reference results match in term of convergence up to 10-02 to 10-08 proves the validity of NN-BLMS. The Optimal Homotopy Asymptotic Method (OHAM) is also used for comparison and to validate the results of NN-BLMM.
Incidence of contrast-induced acute kidney injury in patients with acute mesenteric ischemia and identification of potential predictive factors
Authors: Augène Emmanuel , Lareyre Fabien , Chikande Julien , Guidi Lucas , Mutambayi Grégoire , Lê Cong Duy , Jean-Baptiste Elixène , Katsiki Niki , Mikhailidis Dimitri P , Raffort Juliette
Published: October 2021

Objective

Contrast-enhanced computed tomography angiography (CTA) is commonly used to investigate acute abdominal conditions, but the risk of contrast-induced acute kidney injury (CI-AKI) has been poorly investigated in patients with acute mesenteric ischemia. The aim of the present study was to evaluate the incidence of CI-AKI in these patients and identify potential predictive factors.

Methods

Patients admitted for acute mesenteric ischemia who had a diagnostic CTA with contrast medium and a follow-up of creatinine concentration were retrospectively included.

Results

Among 53 patients included, 9 (16.9%) developed CI-AKI. The prevalence of chronic kidney disease did not differ significantly between those who developed CI-AKI and those who did not (33.3 vs 18.2%, p=.372). Plasma total bilirubin and conjugated bilirubin levels were significantly higher in patients who developed CI-AKI (17.5 vs 8.0 μmol/L, p=.013 and 8.0 vs 3.0 μmol/L, p=.031, respectively). The proportion of patients who had revascularization was similar between patients who developed CI-AKI and those who did not (11.1 vs 20.5%, p>.999). No significant difference was observed for 30-day mortality and all-cause mortality for a median follow-up of 168 days (22.2 vs 13.6%, p=.611; and 33.3 vs 61.4%, p=.153, respectively).

Conclusion

This study reports the incidence of CI-AKI in patients with acute mesenteric ischemia after diagnostic CTA with contrast medium. Plasma bilirubin levels were a predictive factor of CI-AKI in these patients. The administration of contrast media during revascularization was not associated with an increased risk of CI-AKI.
Secondary mechanisms of injury and viable pathophysiological targets in intracerebral hemorrhage
Authors: Bautista Wendy , Adelson P. David , Bicher Nathan , Themistocleous Marios , Tsivgoulis Georgios , Chang Jason J.
Published: October 2021

Intracerebral hemorrhage (ICH) can be divided into a primary and secondary phase. In the primary phase, hematoma volume is evaluated and therapies are focused on reducing hematoma expansion. In the secondary, neuroprotective phase, complex systemic inflammatory cascades, direct cellular toxicity, and blood-brain barrier disruption can result in worsening perihematomal edema that can adversely affect functional outcome. To date, all major randomized phase 3 trials for ICH have targeted primary phase hematoma volume and incorporated clot evacuation, intensive blood pressure control, and hemostasis. Reasons for this lack of clinical efficacy in the major ICH trials may be due to the lack of therapeutics involving mitigation of secondary injury and inflexible trial design that favors unilateral mechanisms in a complex pathophysiology. Potential pathophysiological targets for attenuating secondary injury are highlighted in this review and include therapies increasing calcium, antagonizing microglial activation, maintaining macrophage M1 versus M2 balance by decreasing M1 signaling, aquaporin inhibition, NKCCl inhibition, endothelin receptor inhibition, Sur1-TRPM4 inhibition, matrix metalloproteinase inhibition, and sphingosine-1-phosphate receptor modulation. Future clinical trials in ICH focusing on secondary phase injury and, potentially implementing adaptive trial design approaches with multifocal targets, may improve insight into these mechanisms and provide potential therapies that may improve survival and functional outcome.
Recent Advance of Domino Michael Reaction in Natural Product Synthesis
Authors: Hagiwara Hisahiro
Published: October 2021

Recent advances in the total syntheses of cyclic natural products and related compounds from 2005 to 2021, which employ domino Michael reactions as key steps, have been reviewed, focusing mainly on the domino Michael reactions catalyzed by organocatalysts.
Catechol-O-Methyltransferase Inhibitors Isolated From Thai Propolis
Authors: Miyata Ryo , Motoyama Tomoharu , Nakano Shogo , Ito Sohei , Mukaide Kazuma , Vongsak Boonyadist , Kumazawa Shigenori
Published: October 2021

Propolis is an aggregate of functional components found in plant resins and has been reported to exhibit a variety of valuable biological activities. This study investigated the inhibitory properties of propolis from Thailand toward human catechol- O-methyltransferase (COMT), a key neurotransmitter involved in Parkinson's disease and depression. Samples collected from Chanthaburi and Chiang Mai exhibited relatively high inhibitory activity against COMT. γ-Mangostin (1) and 6-prenyleriodictyol (3) were identified as COMT inhibitors with IC50 values of 62 and 75 μM, respectively. In an enzyme inhibition assay, 1 exhibited mixed inhibition toward COMT. The results suggest that both 1 and propolis have potential applications in the prevention and treatment of psychological illness.
Did the Poona Pact Disenfranchise the ‘Depressed Classes’? An Analysis of the 1936–1937 and 1945–1946 Provincial Elections
Authors: Biswas Sujay
Published: October 2021

This article contests the conventional view that the ‘Depressed Classes’ lost out on representation by agreeing to joint electorates in the Poona Pact. It analyses the results of the elections to the provincial legislatures in British India that took place in 1936–1937 and 1945–1946 under the Government of India Act, 1935, to concretely appraise the working of the Poona Pact. The article argues that reserved seats, primary elections and cumulative voting redeemed the ability of the Poona Pact to provide both descriptive and substantive representation for the ‘Depressed Classes’.
Acute Versus Delayed Surgical Intervention in Multiligament Knee Injuries: A Systematic Review
Authors: Marder Ryan S. , Poonawala Husain , Pincay Jorge I. , Nguyen Frank , Cleary Patrick F. , Persaud Christine S. , Naziri Qais , Zikria Bashir A.
Published: October 2021

Background:

The optimal timing of surgical intervention for multiligament knee injuries remains controversial.

Purpose:

To review the clinical and functional outcomes after acute and delayed surgical intervention for multiligament knee injuries.

Study Design:

Systematic review; Level of evidence, 4.

Methods:

We performed a search of the PubMed, Embase, Cochrane Library, and Web of Science databases from inception to September 2020. Eligible studies reported on knee dislocations, multiligament knee injuries, or bicruciate ligament injuries in adult patients (age, ≥18 years). In addition to comparing outcomes between acute and delayed surgical intervention groups, we conducted 3 subgroup analyses for outcomes within isolated knee injuries, knee injuries with concomitant polytrauma/fractures, and high-level (level 2) studies.

Results:

Included in the analysis were 31 studies, designated as evidence level 2 (n = 3), level 3 (n = 8), and level 4 (n = 20). These studies reported on 2594 multiligament knee injuries sustained by 2585 patients (mean age, 25.1-65.3 years; mean follow-up, 12-157.2 months). At the latest follow-up timepoint, the mean Lysholm (n = 375), International Knee Documentation Committee (IKDC) (n = 286), and Tegner (n = 129) scores for the acute surgical intervention group were 73.60, 67.61, and 5.06, respectively. For the delayed surgical intervention group, the mean Lysholm (n = 196), IKDC (n = 172), and Tegner (n = 74) scores were 85.23, 72.32, and 4.85, respectively. The mean Lysholm (n = 323), IKDC (n = 236), and Tegner (n = 143) scores for our isolated subgroup were 83.7, 74.8, and 5.0, respectively. By comparison, the mean Lysholm (n = 270), IKDC (n = 236), and Tegner (n = 206) scores for the polytrauma/fractures subgroup were 83.3, 64.5, and 5.0, respectively.

Conclusion:

The results of our systematic review did not elucidate whether acute or delayed surgical intervention produced superior clinical and functional outcomes. Although previous evidence has supported acute surgical intervention, future prospective randomized controlled trials and matched cohort studies must be completed to confirm these findings.
Knots Tied With High–Tensile Strength Tape Biomechanically Outperform Knots Tied With Round Suture
Authors: Hong Chih-Kai , Chuang Hao-Chun , Hsu Kai-Lan , Kuan Fa-Chuan , Chen Yueh , Yeh Ming-Long , Su Wei-Ren
Published: October 2021

Background:

Tape-type suture material is well-accepted in arthroscopy surgery.

Purpose:

To compare the knot security of a high–tensile strength round suture and high–tensile strength tape with commonly used arthroscopic knots.

Study Design:

Controlled laboratory study.

Methods:

We compared the performance of No. 2 braided nonabsorbable high-strength suture with that of 1.3-mm braided nonabsorbable high-strength tape. Five commonly used arthroscopic knots were investigated: the Roeder knot; the Western knot; the Samsung Medical Center (SMC) knot; the Tennessee knot; and a static surgeon’s knot. Seven knots were tied for each combination of knots and suture types. Knots were tied on a 30-mm circumferential metal post, and the suture loops were transferred to a materials testing machine. After preloading to 5 N, all specimens were loaded to failure. The clinical failure load, defined as the maximal force to failure at 3 mm of crosshead displacement, yield load, and stiffness, were recorded. A 2-way analysis of variance was used to determine differences between the groups.

Results:

Both suture type and knot type significantly affected the clinical failure load, yield load, and stiffness ( P = .002). The high-strength tape resulted in a significantly greater clinical failure load than the high-strength suture in the case of the Roeder knot, Western knot, and SMC knot ( P = .027, .005, and .016, respectively). When the high-strength round suture was used, the Roeder knot, Western knot, and SMC knot resulted in significantly smaller clinical failure loads compared with the Tennessee knot ( P = .011, .003, and .035, respectively) and the static surgeon’s knot ( P < .001 for all). When the high-strength tape was used, the Roeder knot, Western knot, and SMC knot resulted in significantly smaller clinical failure loads compared with the static surgeon’s knot ( P = .001, .001, and .003, respectively).

Conclusion:

The results of this study indicated that arthroscopic knots tied using 1.3-mm high-strength tape biomechanically outperformed knots tied using a No. 2 high-strength suture. While the static surgeon’s knot exhibited the best biomechanical properties, the Tennessee knot resulted in generally better biomechanical properties among the arthroscopic sliding knots.

Clinical Relevance:

Elongation and loosening of tied knots possibly affects the clinical results of repaired constructs.
Application of Machine Learning Algorithms to Predict Clinically Meaningful Improvement After Arthroscopic Anterior Cruciate Ligament Reconstruction
Authors: Kunze Kyle N. , Polce Evan M. , Ranawat Anil S. , Randsborg Per-Henrik , Williams Riley J. , Allen Answorth A. , Nwachukwu Benedict U. ,   HSS ACL Registry Group, Pearle Andrew , Stein Beth S. , Dines David , Kelly Anne , Kelly Bryan , Rose Howard , Maynard Michael , Strickland Sabrina , Coleman Struan , Hannafin Jo , MacGillivray John , Marx Robert , Warren Russell , Rodeo Scott , Fealy Stephen , O’Brien Stephen , Wickiewicz Thomas , Dines Joshua S. , Cordasco Frank , Altcheck David
Published: October 2021

Background:

Understanding specific risk profiles for each patient and their propensity to experience clinically meaningful improvement after anterior cruciate ligament reconstruction (ACLR) is important for preoperative patient counseling and management of expectations.

Purpose:

To develop machine learning algorithms to predict achievement of the minimal clinically important difference (MCID) on the International Knee Documentation Committee (IKDC) score at a minimum 2-year follow-up after ACLR.

Study Design:

Case-control study; Level of evidence, 3.

Methods:

An ACLR registry of patients from 27 fellowship-trained sports medicine surgeons at a large academic institution was retrospectively analyzed. Thirty-six variables were tested for predictive value. The study population was randomly partitioned into training and independent testing sets using a 70:30 split. Six machine learning algorithms (stochastic gradient boosting, random forest, neural network, support vector machine, adaptive gradient boosting, and elastic-net penalized logistic regression [ENPLR]) were trained using 10-fold cross-validation 3 times and internally validated on the independent set of patients. Algorithm performance was assessed using discrimination, calibration, Brier score, and decision-curve analysis.

Results:

A total of 442 patients, of whom 39 (8.8%) did not achieve the MCID, were included. The 5 most predictive features of achieving the MCID were body mass index ≤27.4, grade 0 medial collateral ligament examination (compared with other grades), intratunnel femoral tunnel fixation (compared with suspensory), no history of previous contralateral knee surgery, and achieving full knee extension preoperatively. The ENPLR algorithm had the best relative performance (C-statistic, 0.82; calibration intercept, 0.10; calibration slope, 1.15; Brier score, 0.068), demonstrating excellent predictive ability in the study’s data set.

Conclusion:

Machine learning, specifically the ENPLR algorithm, demonstrated good performance for predicting a patient’s propensity to achieve the MCID for the IKDC score after ACLR based on preoperative and intraoperative factors. The femoral tunnel fixation method was the only significant intraoperative variable. Range of motion and medial collateral ligament integrity were found to be important physical examination parameters. Increased body mass index and prior contralateral surgery were also significantly predictive of outcome.
The Forgotten 20%: Achievement and Growth in Rural Schools Across the Nation
Authors: Johnson Angela , Kuhfeld Megan , Soland James
Published: October 2021

Leveraging achievement data measured in the fall and spring of kindergarten through eighth grade for 840,000 students attending 8,800 public schools, we report novel evidence on how achievement and growth patterns differ between rural and nonrural schools. Rural students start kindergarten slightly ahead of nonrural students but fall behind by middle school. The divergence is driven by larger summer losses for rural students. In both rural and nonrural schools, we provide additional evidence that Black–White achievement gaps widen during the school year. These findings highlight the importance of seasonal learning patterns in interpreting rural school performance.
Preferences for a Multimedia Web Platform to Increase Awareness of Cultural Heritage: A Stated Choice Experiment
Authors: Wang Benshuo , de Vries Bauke , Dane Gamze
Published: October 2021

As stated by UNESCO, cultural heritage (CH) (tangible and intangible) plays an important role in inheriting, maintaining and passing the values and knowledge from past generations to the next ones. To create an interest and raise the awareness of CH, variety of media sources (i.e., maps, text, 3D models, virtual reality) are exploited. These multimedia sources are brought together on web platforms that preserve and disseminate tangible and intangible CH information, with the aim to reach to large audiences. Although there are many examples of these multimedia web platforms, there is little research on understanding people’s willingness to use such multimedia web platforms and which media type people prefer for understanding and learning about CH. This is important to address since the success and sustainability of such platforms lies on their acceptance by the target audience in terms of data representation and the ease of information provision. To address this problem, this research applied a stated choice experiment to represent a hypothetical multimedia web platform to respondents. Different media types were tested for the description of CH (spatial content and historical content). The collected data from 630 respondents was analysed by a mixed logit model in order to determine the preference towards different media in a given hypothetical multimedia web platform to increase awareness of CH. The results indicate that people prefer multiple media rather than a single medium. Especially, adding dynamic media (i.e., 3D models and videos) to static media (i.e., 2D map and text) increase people’s willingness to use the multimedia web platform. The results help to formulate a new multimedia web platform and can help representatives of heritage sites to create a more sustainable way to broadcast information about CH to the public.
Medial Patellofemoral Ligament Reconstruction
Authors: Wright Margaret L. , Paul Ryan W. , Freedman Kevin B.
Published: October 2021

Background:

Patellar instability is a relatively common condition in the young, active population and causes disruption of the medial patellofemoral ligament (MPFL). MPFL reconstruction is often performed to restore this medial stabilizer and reduce the risk of recurrent instability.

Indications:

Isolated MPFL reconstruction has been shown to reduce the risk of recurrent patellar dislocation. It is indicated in our patients who have had more than 1 dislocation in the absence of other significant bony malalignment or cartilage defects that require concurrent surgery.

Technique Description:

Diagnostic arthroscopy is first performed to evaluate the patellar and trochlear cartilage surfaces. A medial approach to the patella is then performed and the 2 free limbs of the allograft are secured to the patella at the 9 to 11 (or 1 to 3) o’clock position. A small approach to the femoral insertion site of the MPFL is performed and confirmed with fluoroscopy, and the graft is secured to the femur with a biotenodesis screw. Postoperative examination confirms improved stability of the patella, and the patient performs a gradual return to play protocol.

Results:

Recent studies have demonstrated overall good clinical outcomes after MPFL reconstruction, with improved patellar stability and high patient satisfaction. One systematic review found an 85% rate of return to sport with a low risk (7%) of recurrent subluxation or dislocation.

Discussion/Conclusion:

MPFL reconstruction is a reliable option for improving patellar stability in patients with recurrent dislocations. We believe our technique, which optimizes the fixation and footprint of the graft on the patella and allows for easy visualization of femoral anatomy on fluoroscopy, can improve the reproducibility of the procedure and provide optimal clinical outcomes.
An interferon-inducible signature of airway disease from blood gene expression profiling
Authors: Yun Jeong H. , Lee Sool , Srinivasa Pooja , Morrow Jarrett , Chase Robert , Saferali Aadbida , Xu Zhonghui , Cho Michael , Castaldi Peter , Hersh Craig P.
Published: October 2021

Background

The molecular basis of airway remodelling in chronic obstructive pulmonary disease (COPD) remains poorly understood. We identified gene expression signatures associated with chest computed tomography (CT) scan airway measures to understand molecular pathways associated with airway disease.

Methods

In 2396 subjects in the COPDGene Study, we examined the relationship between quantitative CT airway phenotypes and blood transcriptomes to identify airway disease-specific genes and to define an airway wall thickness (AWT) gene set score. Multivariable regression analyses were performed to identify associations of the AWT score with clinical phenotypes, bronchial gene expression and genetic variants.

Results

Type 1 interferon (IFN)-induced genes were consistently associated with AWT, square root wall area of a hypothetical airway with 10 mm internal perimeter (Pi10) and wall area percentage, with the strongest enrichment in AWT. A score derived from 18 genes whose expression was associated with AWT was associated with COPD-related phenotypes including reduced lung function (forced expiratory volume in 1 s percentage predicted β= −3.4; p<0.05) and increased exacerbations (incidence rate ratio 1.7; p<0.05). The AWT score was reproducibly associated with AWT in bronchial samples from 23 subjects (β=3.22; p<0.05). The blood AWT score was associated with genetic variant rs876039, an expression quantitative trait locus forIKZF1, a gene that regulates IFN signalling and is associated with inflammatory diseases.

Conclusions

A gene expression signature with IFN-stimulated genes from peripheral blood and bronchial brushings is associated with CT AWT, lung function and exacerbations. Shared genes and genetic associations suggest viral responses and/or autoimmune dysregulation as potential underlying mechanisms of airway disease in COPD.
Blood eosinophils as a predictor of treatment response in adults with difficult-to-treat chronic cough
Authors: Rybka-Fraczek Aleksandra , Dabrowska Marta , Grabczak Elzbieta M. , Bialek-Gosk Katarzyna , Klimowicz Karolina , Truba Olga , Nejman-Gryz Patrycja , Paplinska-Goryca Magdalena , Krenke Rafal
Published: October 2021

There is lack of evidence on the role of blood eosinophil count (BEC) as a predictor of treatment response in patients with chronic cough. The study aimed to evaluate BEC as a predictor of treatment response in all non-smoking adults with chronic cough and normal chest radiograph referred to cough clinic and in a subgroup of patients with chronic cough due to asthma or non-asthmatic eosinophilic bronchitis (NAEB).

This prospective cohort study included 142 consecutive, non-smoking patients referred to our cough centre due to chronic cough. The management of chronic cough was performed according to the current recommendations. At least a 30-mm decrease of 100-mm visual analogue scale in cough severity and a 1.3 points improvement in Leicester Cough Questionnaire were classified as a good therapeutic response.

There was a predominance of females (72.5%), median age 57.5 years with long-lasting, severe cough (median cough duration 60 months, severity 55/100 mm). Asthma and NAEB were diagnosed in 47.2% and 4.9% of patients, respectively. After 12–16 weeks of therapy, a good response to chronic cough treatment was found in 31.0% of all patients. A weak positive correlation was demonstrated between reduction in cough severity and BEC (r=0.28, p<0.001). Area under the curve for all patients with chronic cough was 0.62 with the optimal BEC cut-off for prediction of treatment response set at 237 cells·µL−1and for patients with chronic cough due to asthma/NAEB was 0.68 (95% CI 0.55–0.81) with the cut-off at 150 cells·µL−1.

BEC is a poor predictor of treatment response in adults with chronic cough treated in the cough centre.
Comparison of resting-state EEG between adults with Down syndrome and typically developing controls
Authors: Hamburg Sarah , Bush Daniel , Strydom Andre , Startin Carla M.
Published: October 2021

Background

Down syndrome (DS) is the most common genetic cause of intellectual disability (ID) worldwide. Understanding electrophysiological characteristics associated with DS provides potential mechanistic insights into ID, helping inform biomarkers and targets for intervention. Currently, electrophysiological characteristics associated with DS remain unclear due to methodological differences between studies and inadequate controls for cognitive decline as a potential cofounder.

Methods

Eyes-closed resting-state EEG measures (specifically delta, theta, alpha, and beta absolute and relative powers, and alpha peak amplitude, frequency and frequency variance) in occipital and frontal regions were compared between adults with DS (with no diagnosis of dementia or evidence of cognitive decline) and typically developing (TD) matched controls (n = 25 per group).

Results

We report an overall ‘slower’ EEG spectrum, characterised by higher delta and theta power, and lower alpha and beta power, for both regions in people with DS. Alpha activity in particular showed strong group differences, including lower power, lower peak amplitude and greater peak frequency variance in people with DS.

Conclusions

Such EEG ‘slowing’ has previously been associated with cognitive decline in both DS and TD populations. These findings indicate the potential existence of a universal EEG signature of cognitive impairment, regardless of origin (neurodevelopmental or neurodegenerative), warranting further exploration.
Data integration and evolutionary analysis of long non-coding RNAs in 25 flowering plants
Authors: Sang Shiye , Chen Wen , Zhang Di , Zhang Xuan , Yang Wenjing , Liu Changning
Published: October 2021

Background

Long non-coding RNAs (lncRNAs) play vital roles in many important biological processes in plants. Currently, a large fraction of plant lncRNA studies center at lncRNA identification and functional analysis. Only a few plant lncRNA studies focus on understanding their evolutionary history, which is crucial for an in-depth understanding of lncRNAs. Therefore, the integration of large volumes of plant lncRNA data is required to deeply investigate the evolution of lncRNAs.

Results

We present a large-scale evolutionary analysis of lncRNAs in 25 flowering plants. In total, we identified 199,796 high-confidence lncRNAs through data integration analysis, and grouped them into 5497 lncRNA orthologous families. Then, we divided the lncRNAs into groups based on the degree of sequence conservation, and quantified the various characteristics of 756 conserved Arabidopsis thaliana lncRNAs. We found that compared with non-conserved lncRNAs, conserved lncRNAs might have more exons, longer sequence length, higher expression levels, and lower tissue specificities. Functional annotation based on the A. thaliana coding-lncRNA gene co-expression network suggested potential functions of conserved lncRNAs including autophagy, locomotion, and cell cycle. Enrichment analysis revealed that the functions of conserved lncRNAs were closely related to the growth and development of the tissues in which they were specifically expressed.

Conclusions

Comprehensive integration of large-scale lncRNA data and construction of a phylogenetic tree with orthologous lncRNA families from 25 flowering plants was used to provide an oversight of the evolutionary history of plant lncRNAs including origin, conservation, and orthologous relationships. Further analysis revealed a differential characteristic profile for conserved lncRNAs in A. thaliana when compared with non-conserved lncRNAs. We also examined tissue specific expression and the potential functional roles of conserved lncRNAs. The results presented here will further our understanding of plant lncRNA evolution, and provide the basis for further in-depth studies of their functions.
Grapevine Rpv3-, Rpv10- and Rpv12-mediated defense responses against Plasmopara viticola and the impact of their deployment on fungicide use in viticulture
Authors: Wingerter Chantal , Eisenmann Birgit , Weber Patricia , Dry Ian , Bogs Jochen
Published: October 2021

Background

The high susceptibility of European grapevine cultivars (Vitis vinifera) to downy mildew (Plasmopara viticola) leads to the intensive use of fungicides in viticulture. To reduce this input, breeding programs have introgressed resistance loci from wild Vitis species into V. vinifera, resulting in new fungus-resistant grapevine cultivars (FRC). However, little is known about how these different resistance loci confer resistance and what the potential reduction in fungicide applications are likely to be if these FRCs are deployed. To ensure a durable and sustainable resistance management and breeding, detailed knowledge about the different defense mechanisms mediated by the respective Rpv (Resistance to P. viticola) resistance loci is essential.

Results

A comparison of the resistance mechanisms mediated by the Rpv3–1, Rpv10 and/or Rpv12-loci revealed an early onset of programmed cell death (PCD) at 8 hours post infection (hpi) in Rpv12-cultivars and 12 hpi in Rpv10-cultivars, whereas cell death was delayed in Rpv3-cultivars and was not observed until 28 hpi. These temporal differences correlated with an increase in the trans-resveratrol level and the formation of hydrogen peroxide shortly before onset of PCD. The differences in timing of onset of Rpv-loci specific defense reactions following downy mildew infection could be responsible for the observed differences in hyphal growth, sporulation and cultivar-specific susceptibility to this pathogen in the vineyard. Hereby, Rpv3- and Rpv12/Rpv3-cultivars showed a potential for a significant reduction of fungicide applications, depending on the annual P. viticola infection pressure and the Rpv-loci. Furthermore, we report on the discovery of a new P. viticola isolate that is able to overcome both Rpv3- and Rpv12-mediated resistance.

Conclusion

This study reveals that differences in the timing of the defense reaction mediated by the Rpv3-, Rpv10- and Rpv12-loci, result in different degrees of natural resistance to downy mildew in field. Vineyard trials demonstrate that Rpv12/Rpv3- and Rpv3-cultivars are a powerful tool to reduce the dependence of grape production on fungicide applications. Furthermore, this study indicates the importance of sustainable breeding and plant protection strategies based on resistant grapevine cultivars to reduce the risk of new P. viticola isolates that are able to overcome the respective resistance mechanism.
Persistent eosinopenia is associated with in-hospital mortality among older patients: unexpected prognostic value of a revisited biomarker
Authors: Partouche Bethsabee , Pepin Marion , de Farcy Pauline Mary , Kahn Jean-Emmanuel , Sawczynski Bruno , Lechowski Laurent , Teillet Laurent , Barbot Frederic , Herr Marie , Davido Benjamin
Published: October 2021

Introduction

Infection is one of the major causes of mortality and morbidity in older adults. Available biomarkers are not associated with prognosis in older patients. This study aimed to analyze the value of eosinopenia (eosinophil count< 100/mm3) as a prognosis marker among older patients with suspected or confirmed bacterial infection.

Methods

A retrospective study was performed from 1 January to 31 December 2018 among patients in a geriatrics ward suffering from a bacterial infection treated with antibiotics. Biomarker data including the eosinophil count, neutrophil count and C-reactive protein (CRP) were collected within 4 days after patient diagnosis. Persistent eosinopenia was defined as a consistent eosinophil count< 100/mm3 between Day 2 and Day 4. The association of biomarkers with 30-day hospital mortality in a multivariate analysis was assessed and their predictive ability using the area under the ROC curve (AUC) was compared.

Results

Our study included 197 patients with a mean age of 90 ± 6 years. A total of 36 patients (18%) died during their stay in hospital. The patients who died were more likely to have persistent eosinopenia in comparison to survivors (78% versus 34%, p < 0.001). In the multivariate analysis, persistent eosinopenia was associated with in-hospital mortality with an adjusted HR of 8.90 (95%CI 3.46–22.9). The AUC for eosinophil count, CRP and neutrophil count between Day 2 and Day 4 were 0.7650, 0.7130, and 0.698, respectively.

Conclusion

Persistent eosinopenia within 4 days of diagnosis of bacterial infection appeared to be a predictor of in-hospital mortality in older patients.
The risk of preterm birth in combinations of socioeconomic position and mental health conditions in different age groups: a Danish nationwide register-based cohort study
Authors: Knudsen Camilla Klinge , Christesen Amanda Marie Somer , Heuckendorff Signe , Fonager Kirsten , Johansen Martin Nygård , Overgaard Charlotte
Published: October 2021

Background

Inequality in preterm birth is a world-wide challenge that has proved difficult for maternity care services to meet. Reducing the inequality requires identification of pregnant women at particularly high risk of preterm birth in order to target interventions. Therefore, the aim was to estimate the risk of preterm birth in women with different combinations of socioeconomic position, mental health conditions, and age.

Methods

In this nationwide register-based cohort study, we included all first-time mothers that gave birth to a singleton liveborn infant in Denmark between 2000 and 2016. The absolute and relative risk of preterm birth (< 37 weeks of gestation) was examined in different combinations of educational level (high, intermediate, and low) and mental health conditions (no, minor, and moderate/severe) in three age strata (≤23, 24–30, and ≥ 31 years). We estimated the relative risk using Poisson regression with a robust error variance. As additive interaction can help identify subgroups where limited resources can be of best use, we measured the attributable proportion to assess the risk that is due to interaction of the different exposures.

Results

Of the 415,523 included first-time mothers, 6.3% gave birth prematurely. The risk of preterm birth increased with decreasing educational level and increasing severity of mental health conditions in all age strata, but most in women aged ≥31 years. The highest absolute risk was 12.9% [95% CI: 11.2;14.8%] in women aged ≥31 years with low education and moderate/severe mental health conditions resulting in a relative risk of 2.23 [95% CI: 1.93–2.58] compared to the unexposed reference group in that age strata. We found positive additive interaction between low education and mental health conditions in women aged 24–30 and ≥ 31 years and between age ≥ 31 years and combinations of mental health conditions and educational levels.

Conclusion

The inequality in preterm birth increased with increasing age. To reduce inequality in preterm birth focused attention on women with higher age further combined with lower educational levels and mental health conditions is essential.
Correlation between low-level viremia and hepatitis B-related hepatocellular carcinoma and recurrence: a retrospective study
Authors: Sun Furong , Liu Zhifei , Wang Bingyuan
Published: October 2021

Background

Low-level viremia generally refers to detectable HBV DNA levels lower than 2000 IU/mL. Studies show that low-level viremia is a risk factor for hepatocellular carcinoma. The aim of this study was to explore the characteristics of low-level viremia patients with hepatitis B-related hepatocellular carcinoma and identify prognostic factors after curative hepatectomy.

Methods

Data from chronic hepatitis B patients with hepatocellular carcinoma receiving curative hepatectomy for the first time in the first hospital of China Medical University were studied. Patients were divided into two groups based on preoperative HBV DNA levels: group 1 (low-level viremia group, HBV DNA < 2000 IU/mL) and group 2 (HBV DNA ≥ 2000 IU/mL).

Results

Of the 212 patients, 104 patients were in group 1 and 108 patients were in group 2. There was a lower proportion of patients with HBsAg levels > 250 IU/mL (the upper limit of detection in our laboratory) in group 1 than in group 2 (71.2% vs. 86.1%, P < 0.01). The percentage of patients with a tumor diameter < 5 cm was 67.3% in group 1 and 37.0% in group 2 (P < 0.000). The percentage of tumor recurrence was 40.4% (42) in group 1 and 54.6% (59) in group 2 (P < 0.05). Median recurrence-free survival was 30.1 months in group 1 and 17.6 months in group 2 (P < 0.01). Multivariate analysis showed that a tumor diameter ≥ 5 cm (hazard ratio [HR] = 1.819, 95% confidence interval [CI] 1.193–2.775, P = 0.005), intrahepatic metastasis (HR = 1.916, 95% CI 1.077–3.407, P = 0.027), and an HBV DNA level ≥ 100 IU/mL (the lower limit of detection in our laboratory, HR = 2.943, 95% CI 1.916–4.520, P < 0.000) were independent prognostic factors associated with an increased risk of hepatocellular carcinoma recurrence.

Conclusion

Preoperative low-level viremia was related with a long tumor recurrence interval and complete virologic response after curative hepatectomy was associated with a lower risk of hepatocellular carcinoma recurrence.
Evidence on article 5.3 of FCTC (tobacco industry interference in tobacco control activities) in India- a qualitative scoping study
Authors: Goel Sonu , Kar Sitanshu Sekhar , Verma Madhur , Sivanantham Parthibane , Naik Bijay Nanda , Gupta Deepti
Published: October 2021

Background

The Tobacco Industry (henceforth TI) yearns to portray itself as being “socially responsible” and fights for the decision-making positions; that are it used to deter, delay or dilute tobacco control measures. There is little documented evidence of Tobacco Industry Interference (henceforth TII) from India, the scope of their interference and challenges faced by the experts for effective tobacco control. This research study seeks to cover this significant gap in the literature on the TI of India.

Methods

A cross-sectional qualitative research design, based upon in-depth interviews (N = 26), was used to explore the key stakeholders’ opinions regarding TII in India. The interviews used a set of questions to collect information about the participant’s roles and responsibilities in tobacco control, the nature of TII faced by the participants, means of influence by TI, barriers and challenges to tobacco control efforts.

Results

Most of the respondents were engaged in tobacco control, training, advocacy and awareness generation activities for 5–10 years or more. The respondents defined the TI and its scope as per their experience with the help of the power ranking methodology. Most of them perceived TI as ‘manufacturers’ while others consider them as ‘advertisers’, ‘public relation companies’, ‘wholesalers’, ‘vendors’, and ‘Government firms with TI stocks. The research team identified six significant domains: influencing the policy and administrative decisions, Interference in the implementation of tobacco control laws and activities, false propaganda and hiding the truth, manipulating front action groups (FAG), rampant tobacco advertising and promotion activities and others under which TII activities were classified. Most respondents believed that TI players were interfering in the policy decisions, implementing the tobacco control laws and activities and manipulating the FAG. A detailed taxonomic classification of the TII strategies that emerged from our analysis was linked to article 5.3 of FCTC.

Conclusions

The study documented a significant level of TII in different domains, with stakeholders acting at various hierarchical levels. Thus providing insight into the tactics of the TI in order to enable stakeholders to anticipate and pre-empt the kinds of alliances the TI may attempt to build; stimulating academicians and researchers to undertake in-depth analysis into various strategies and therefore underscoring the need to ensuring transparency in official interaction with the TI and its representatives.
Does the rate of orthodontic tooth movement change during the estrus cycle? A systematic review based on animal studies
Authors: Almidfa Noura Saeed Sultan , Athanasiou Athanasios E. , Makrygiannakis Miltiadis A. , Kaklamanos Eleftherios G.
Published: October 2021

Background

As the fluctuation of sex hormone levels in menstruating women results in periodical effects in bone metabolism, understanding the implications for tooth movement could be of benefit to the orthodontist. This type of research presents practical and ethical problems in humans, but animal models could provide useful information. Our objective was to systematically investigate the available evidence on the question whether the rate of orthodontic tooth movement varies between the different stages of the estrus cycle in animals.

Methods

Unrestricted searches in 7 databases and manual searching of the reference lists in relevant studies were performed up to February 2021 (Medline [PubMed], CENTRAL [Cochrane Library; includes records from Embase, CINAHL, ClinicalTrials.gov, WHO's ICTRP, KoreaMed, Cochrane Review Groups’ Specialized Registers, and records identified by handsearching], Cochrane Database of Systematic Reviews [Cochrane Library], Scopus, Web of Knowledge [including Web of Science Core Collection, KCI Korean Journal Database, Russian Science Citation Index, SciELO Citation Index and Zoological Record], Arab World Research Source [EBSCO] and ProQuest Dissertation and Theses [ProQuest]). Our search focused on prospective controlled animal studies, whose samples included female subjects of any species that were quantitatively comparing the amount of tooth movement in the different stages of the estrus cycle. Following study retrieval and selection, relevant data was extracted, and the risk of bias was assessed using the SYRCLE’s Risk of Bias Tool.

Results

From the finally assessed records, 3 studies met the inclusion criteria. Two of the studies experimented on Wistar rats, whereas the other on cats. Tooth movement was induced by expansion or coil springs. The rate of orthodontic tooth movement was increased during the stages of the estrus cycle when oestrogen and/or progesterone levels were lower. The risk of bias in the retrieved studies was assessed to be unclear.

Conclusion

Hormonal changes during the estrus cycle may affect the rate of orthodontic tooth movement. Although these animal experiment results should be approached cautiously regarding their translational potential, it could be useful to consider the possible impact of these physiological changes in the clinical setting until more information becomes available.

Registration: PROSPERO (CRD42021158069).