bluuebunny/crossref_metadata_2025 huggingface dataset:
Production of starch nanoparticles through solvent-antisolvent precipitation in a spinning disc reactor
Authors: Sana Sahr , Boodhoo Kamelia , Zivkovic Vladimir
Published: May 2019

The spinning disc reactor (SDR) uses surface rotation to produce thin film flow with improved mixing and reduced residence times in chemical processing applications. Solvent-antisolvent precipitation is one such process that can benefit from these properties. This study investigates the film hydrodynamics and precipitation of starch nanoparticles by contacting starch dissolved in sodium hydroxide with ethanol as the antisolvent. One objective of this study is to understand how interactions of the disc surface topography (grooved and smooth) with other parameters such as liquid flowrate, antisolvent to solvent flow ratio and disc speed impact the mixing and precipitation processes. Results indicate that an increase in flow rate and rotational speed leads to smaller nano-particles and narrower size distributions, which is attributed to increased shear and instabilities within the liquid film. It was also observed that an increased antisolvent to solvent ratio caused a reduction in particle size, as increased antisolvent generated higher supersaturation. Results showed that although particle size was not significantly influenced by the disc texture, the size distribution was narrower and higher yields were obtained with the grooved disc surface. The grooved disc therefore offers the opportunity for higher throughput in the solvent-antisolvent precipitation of starch particles with better product quality.
Creep behaviour and life assessment of a cast nickel – base superalloy MAR – M247
Authors: Kvapilova Marie , Dvorak Jiri , Kral Petr , Hrbacek Karel , Sklenicka Vaclav
Published: May 2019

The cast nickel-base MAR-M247 superalloy has been widely used for high-temperature components. In this work, the creep behaviour of two alternates of MAR-M247 superalloy with different grain size processed at different temperatures of casting are compared. Under the creep testing conditions used in this study, only negligible differences of creep behaviour of the alternate alloys were found and the evaluated creep characteristics correspond to the power-law or dislocation creep. The microstructure of the alloys consists of a 𝛾 matrix with a eutectic, 𝛾'
strengthening cubic precipitates, and M6C and M23C6 carbides. Increasing the temperature induces the dissolution of some M23C6 carbides. Fractures of both variants of alloys exhibit a more ductile character at higher temperatures, while at lower temperature a mixture of brittle and ductile fracture modes was observed, which changes the creep fracture ductility.
The refusal of request speech act in Persian, English, and Balouchi languages: A cross-cultural and cross-linguistic study
Authors: Moafian Fatemeh , Yazdi Naji , Sarani Abdullah
Published: May 2019

The study examined the realization of refusal of request speech act in Persian, English, and Balouchi languages. 219 individuals participated in the study. Discourse completion task was employed to elicit the participants’ refusals. Descriptive statistics and Chi-square were used to analyze the data. The findings revealed the existence of statistically significant differences among the three groups of speakers concerning both the total frequencies and the frequencies of direct, indirect, and adjuncts to refusals strategies. Furthermore, concerning social status, no statistically significant differences were detected either for total number of strategies or for the number of strategies in main categories in each language, except for the indirect strategies among Persian speakers. In Persian language, with the increase in the interlocutors’ social status level, the increase in the number of indirect strategies was discerned. Likewise, the frequency differences of some semantic formulas were statistically significant regarding the social status in each language.
Entropy and Entropy Production in Multiscale Dynamics
Authors: Grmela Miroslav , Pavelka Michal , Klika Václav , Cao Bing-Yang , Bendian Nie
Published: May 2019

Heat conduction is investigated on three levels: equilibrium, Fourier, and Cattaneo. The Fourier level is either the point of departure for investigating the approach to equilibrium or the final stage in the investigation of the approach from the Cattaneo level. Both investigations bring to the Fourier level an entropy and a thermodynamics. In the absence of external and internal influences preventing the approach to equilibrium the entropy that arises in the latter investigation is the production of the classical entropy that arises in the former investigation. If the approach to equilibrium is prevented, then the entropy that arises in the investigation of the approach from the Cattaneo level to the Fourier level still brings to the Fourier level the entropy and the thermodynamics even if the classical entropy and the classical thermodynamics are absent. We also note that vanishing total entropy production as a characterization of equilibrium state is insufficient.
The semantics of Scandinavian pancake constructions
Authors: Haugen Tor Arne , Enger Hans-Olav
Published: May 2019

A classical topic in the syntax of the mainland Scandinavian languages is so-called pancake clauses where there seemingly is disagreement between the subject and the predicative adjective, as in Pannekaker er godt ‘Pancakes(f):indf:pl be:prs good:n:sg’; the subject is in the plural, whereas the predicative adjective is in the neuter singular. According to one of the several approaches, these clauses display a type of semantic agreement. Recently, it has also been argued that there are at least four different types of pancake constructions.

In this article, the semantic relationship between the different constructions is investigated further. It is argued that, diachronically, pancake agreement started with subjects interpreted as virtual, ungrounded processes, and that the absence of grounding has been reinterpreted as absence of spatial boundedness in the latest kind of pancake construction. The analysis is supported by a diachronic corpus investigation. The emphasis on virtual reference is a new feature with the current paper, and it enables us to set aside an objection against the semantic agreement analysis. The diachronic corpus investigation enables us to revise, empirically, earlier suggestions as to when the pancake constructions originated: They are well attested from the mid-1800s, in both Swedish and Norwegian Nynorsk.
Variationist typology: Shared probabilistic constraints across (non-)null subject languages
Authors: Torres Cacoullos Rena , Travis Catherine E.
Published: May 2019

A key parameter in received classifications of language types is the expression of pronominal subjects. Here we compare variation patterns in conversational data of English – considered a non-null-subject language – and Spanish – a well-studied null-subject language. English has a patently lower rate of expression (approximately 3% unexpressed 1sg and 3sg human subjects vs. 60% in Spanish). Despite the stark difference in rate of expression, the same probabilistic constraints are at work in the two languages. Contrary to popular belief, VP coordination is neither a discrete nor a distinguishing category of English. Instead, a shared constraint is linking with the preceding subject, a refinement of accessibility to include, alongside coreferentiality, measures of structural connectedness – both prosodic and syntactic. Other shared constraints on unexpressed subjects are coreferential subject priming (a tendency to repeat the form of the previous mention) and lexical aspect (reflecting the contribution of a temporal relationship to subject expression). Where the languages most differ is in the envelope of variation. In English, besides coreferential-subject verbs conjoined with a coordinating conjunction, unexpressed subjects are limited to prosodic initial-position in declarative main clauses, a restriction that is absent in Spanish. We propose that the locus of cross-language comparisons is the variable structure of each language, defined by the set of probabilistic constraints but also the delimitation of the variable context within which these are operative.
Art, Ethnic Identity and Frozen Actions: Conceptualising Art Created and Displayed in the Home of a Māori Visual Artist and a Samoan Visual Artist
Authors: Matelau-Doherty Tui
Published: May 2019

Defining and understanding a positive and inclusive Māori or Pacific ethnic identity is difficult. Yet doing so is necessary in order to enhance the wellbeing of Māori and Pacific people in New Zealand. This paper argues that analysing the art of a Māori and Samoan visual artist using frozen actions as the analytical tool, reveals their fluid ethnic identity. Actions such as hanging the art, producing the art and researching the art are embedded as frozen actions in the art itself. These identity telling actions reveal a fluid ethnic identity, a positive and inclusive ethnic identity which combines ideas about the social environment and ethnicity.
What makes (im)politeness for travellers?Spanish tourists’ perceptions at national and international hotels
Authors: López María de la O Hernández , Amaya Lucía Fernández
Published: May 2019

Although hotel service encounters have been widely studied, the literature presents two main shortcomings: 1) receptionist-guest communication has not received much attention from a politeness perspective; and 2) the scarcity of studies regarding politeness1 (i. e., the interlocutors’ perception of politeness) has been overshadowed by the vast amount of literature concerning politeness2. Regarding customer perception, understanding what appropriate communicative behaviour is - i. e. whether (non)verbal actions are conducive to variation in terms of customer perception and satisfaction - is crucial to understand service success or failure. In this context, our study examines the opinion and perception of 183 Spanish participants who had stayed at national and international hotels and who assessed, on the one hand, to what extent the receptionists were polite or impolite, and why; and on the other, how communication with customers could improve in terms of politeness. The findings indicate that, despite the participants’ general preference for friendliness and solidarity, the politeness strategies that participants valued as adequate in this context are not necessarily those traditionally related to Peninsular Spanish (i. e. directness and/or involvement). Also, this study shows that what lay people consider politeness encompasses a great number of variables in which linguistic production is but one of them.
Impoliteness in English and Chinese online diners’ reviews
Authors: Lai Xiaoyu
Published: May 2019

Linguistic impoliteness appears to be an important feature of online reviews. This paper examines how impoliteness is realized in English and Chinese negative reviews, and how these reviews are responded to. The data sets are composed of 32 English and 32 Chinese negative reviews with responses respectively from the websitesTripAdvisorandDazhongdianping. Building on the work of Culpeper (1996; 2011), English reviewers are found to adopt more of a mixture of approval and criticism, and “stuff-oriented” pointed complaints, while Chinese reviewers employ more indignant exclamations and “staff-oriented” pointed complaints. This suggests that the latter are more concerned about their own face needs, and thus have the potential to be perceived as more impolite and aggravating than the former. However, it is also found that Chinese respondents are potentially more polite and indirect than English respondents. Chinese respondents pay more attention to reviewers’ face wants rather than that of their own, while English respondents attach greater importance to maintaining a positive image for a restaurant, and hence attending to their own face needs. This paper reveals a sharp contrast in the use of impoliteness and its link to the concept of face in English and Chinese negative reviews and responses.
An Intergenerational Perspective on (Im)politeness
Authors: Bella Spyridoula , Ogiermann Eva
Published: May 2019

The present paper provides an intergenerational perspective on Greek conceptualizations of (im)politeness. Based on interviews eliciting narratives of impolite behaviour of our participants’ parents’ generation, the study illustrates the contested and changing nature of politeness in contemporary Greece.

Through critically evaluating the older generation’s behaviour, the participants not only provided insights into their own politeness norms but also showed a clear understanding of the previous generation’s politeness norms. The discrepancy between what is perceived as polite by the two generations points to a distinction between empirical (is) and moral (should) norms (Haugh 2010), with the former allowing the participants to classify their parents’ impoliteness as non-intentional and the latter reflecting the emergence of new conceptualizations of politeness in Greece.

While Greece has been unanimously characterized as a positive politeness culture in previous research, the present study illustrates an increasing emphasis on values and norms associated with negative politeness.
Gerunds become prepositional infinitives in Romance Small Clauses: The effects of later Merge to the syntactic spine
Authors: Casalicchio Jan
Published: May 2019

This article offers a comparative analysis of “predicative” gerunds (“PGs”) and prepositional infinitives (“PIs”), focussing on perception constructions in Spanish (PGs) and European Portuguese (PIs). I demonstrate that these two constructions are diachronically related and that they still have a similar syntax. The evidence discussed suggests that both constructions are Small Clauses headed by a preposition of central coincidence. In PGs, this preposition is merged in a low aspectual projection and incorporates into the verb. In PIs, an evolution of PGs, it is merged later in the structure. This leads to a layering process, i.e. the splitting of a single head into a series of heads and transferring the semantics of one functional head to more heads. Thus, PIs have a more expanded structure than gerunds. Finally, further empirical evidence for this analysis is discussed, such as the insertion of adverbs and the use ofsin(“without”) to negate the perceived event.
Valuation of Medieval Churches; Taking Account of Laypersons’ Views
Authors: van der Schoor Tineke , Colmenero-Acevedo Ezequiel , Vieveen Maarten
Published: May 2019

Local voluntary groups often play an important role in the day-to-day care for historic churches, therefore the perspective of laypersons is very relevant for their conservation. In this paper, we investigate laypersons’ valuation of historic buildings, their experiences of thermal comfort in those buildings and contrast this with their views on the appropriateness of energy efficiency measures. This paper presents four case studies of medieval churches in Groningen, Netherlands. We applied interviews and photo-elicitation to investigate the values held by local committees that take daily care of the churches in our sample. Our theoretical contribution lies in the combination of valuation studies and heritage approaches. Valuation studies is used to investigate the values that are attached to historic buildings by various stakeholders. We apply the ‘heritage-as–a-spatial-vector’ approach which focuses on using heritage as a resource and to position heritage in relation to developments in society. We conclude that for a more balanced assessment of historic buildings, laypersons’ valuations should be further integrated in heritage studies. In particular, community values and comfort needs should be more fully addressed in value assessments.
Guidance for Finding a Sustainable Balance between Energy Savings and Heritage Preservation When Retrofitting Heritage Buildings
Authors: Héberlé E. , Borderon J. , Burgholzer J.
Published: May 2019

A guidance was developed to find a sustainable balance between energy savings and heritage preservation when retrofitting heritage buildings. It was applied in a study on the retrofitting of typical Alsatian heritage buildings. Seven buildings were analysed before retrofitting to evaluate them on five criteria: energy savings, heritage preservation but also comfort in winter and in summer and moisture damage. Then, compatible retrofitting works on walls, ceilings, floors, front door, windows, ventilation and heating and hot production water systems were selected in order to create three retrofitting scenarios: a high-energy efficiency scenario with moisture-permeable materials, a balance scenario between energy efficiency and heritage preservation and a high heritage preservation scenario. The results of the study showed that these sustainable retrofitting scenarios preserve heritage while saving energy and are available in a web publication headed to Alsatian private individuals. The guidance in itself can be easily replicated to other types of heritage buildings.
Building Performance Evaluation – A Design Approach for Refurbishment of a Small Traditional Building in Scotland
Authors: Foster Janice A. , Poston Anna , Foster Samuel A.
Published: May 2019

In recent years, thermal performance improvements have been applied to an increasing number of historic buildings towards the achievement of the legally binding Scottish carbon dioxide (CO2) emission reductions. Over 20 % of the built environment in Scotland was constructed pre 1919 and the targeting of fabric improvements in these buildings can pose a performance risk if inappropriate measures are applied. This paper discusses through a case study a Building Performance Evaluation (BPE) approach used in conjunction with the design process for refurbishment of a community owned historic building, located in Arisaig, Scotland. The community received funding to improve the energy performance of this nineteenth century stone building and committed to a 75 % reduction in CO2 emissions. BPE was conducted in 2014 as part of the design process and repeated post-refurbishment in 2015 to validate the design. The initial BPE identified high heat losses, inefficient heating and lighting systems that resulted in occupant discomfort, high running costs and consequently the loss of a community facility during the winter months. The resulting BPE quantified improvements to the building fabric, occupant comfort and reduced energy consumption, which advocated this design approach as a beneficial tool for informing historic building refurbishment.
An Investigation of the Energy Efficiency of Traditional Buildings in the Oporto World Heritage Site
Authors: Flores Joaquim Moura
Published: May 2019

Oporto’s traditional buildings are the major contributors for shaping the World Heritage Site Despite this, and as is the case in most European historic cities, they are not individually listed and any adaptations to make them comply with current energy efficiency requirements may cause negative impacts on their authenticity and integrity. This paper aims to identify which energy efficiency improvement measures can be applied without damaging the buildings’ heritage value. For this purpose, fieldwork and simulation data of ten case studies were used. On-site results revealed that the energy consumption in Oporto’s traditional buildings was below European average and the households expressed that their home comfort sensation was overall positive. Simulations showed that introducing insulation and solar thermal panels would be ineffective in terms of energy and cost efficiency as well as comfort improvement.

This study reinforces the idea that traditional buildings perform better than expected in terms of energy consumption and can be retrofitted and updated at a low-cost and with passive solutions.
‘You’ll need help from your adult assistant’: Readership accommodation in children’s recipes
Authors: Brunner Marie-Louise
Published: May 2019

This article investigates how children’s recipes are adapted structurally and linguistically for their young readership. It is based on comparative data, using 24 children’s and 24 adults’ versions of the same recipes, and additional online and printed children’s recipes as reference. Even though recipes have been researched extensively, research on recipes for children in particular is rare. Based on general research on facilitation methods in recipe writing, the article analyzes how children, as the target readership, are accommodated in the analyzed recipes and how they differ from recipes intended for adults. Findings suggest that children’s recipes often have an appealing title, are more detailed and clearer, and use visual support. There is a tendency to reduce presuppositions and include additional advice. However, they seem less consistent than adult recipes and, in contrast to the general trend for accommodation, often use technical terminology without further explanations. The request for an adult helper distinguishes children’s from adults’ recipes and serves as a differentiating feature. These findings indicate that children’s recipes are adapted to the particular context and needs of a young and inexperienced audience.
A corpus-based study on the functions of antonym co-occurrences in spoken Chinese
Authors: Hsu Chan-Chia
Published: May 2019

Previous corpus-based research has demonstrated that antonyms co-occur frequently and serve essential functions in discourse. However, these studies are mostly based on written corpus data. Therefore, the present study investigates how antonyms are used in spoken Chinese. Antonyms co-occurring within five turns were manually identified in the National Chengchi University (NCCU) Corpus of Spoken Taiwan Mandarin (27 transcripts, approximately 11 hours) and categorized by their functions. It is found that antonyms that are dialogic in nature prevail in spoken Chinese, and the results reconfirm that antonyms are often used to signal a nearby contrast or to express inclusiveness/exhaustiveness. Compared with written Chinese, spoken Chinese shows a stronger preference for three functional categories, i.e. Interrogative Antonymy, Corrective Antonymy, and Negated Antonymy, which clearly reflect the spontaneous, interactive nature of conversation. The comparison between spoken and written Chinese also shows that antonyms in spoken Chinese co-occur in particular lexico-syntactic frames less often, and that the morphosyllabic structure of antonyms, a crucial factor that influences the functional distribution of antonyms in written Chinese, occupies a minor role in spoken Chinese. This study reveals how the use of antonyms varies across spoken and written Chinese, complementing previous corpus-based studies of antonymy that have drawn conclusions mostly from formal written texts.
Response of a High Damping Rubber Bearing to Multiaxial Excitation
Authors: Chen Michelle C. , Restrepo José I. , Benzoni Gianmario
Published: May 2019

A high damping rubber bearing fabricated as part of a seismic isolation system was tested at the Caltrans Seismic Response Modification Device Test Facility at the University of California, San Diego. The purpose of the test program, which consisted of 33 tests, was to assess the impact of multiaxial excitation and input rate on the response of the bearing. The test protocol consisted of unique tests, including testing the bearing in all six degrees of freedom (uncoupled and coupled) and testing the bearing dynamically to failure. This article examines the hysteretic response and resulting performance parameters of the bearing to the different tests. The most notable impacts on the bearing’s hysteretic response were the axial loading and coupling the lateral degrees of freedom, while the rotational degrees of freedom had minimal impact on the bearing’s response. The bearing’s hysteretic response to coupling input in the rotational degrees of freedom with lateral and axial degrees of freedom was consistent with the expected performance from superimposing the response to input from individual degrees of freedom. The bearing failed at almost three times the design shear strain and at a shear force over five times the shear force at the design shear strain during the dynamic test to failure.
Christianizing the Planetary Week and Globalizing the Seven-Day Cycle
Authors: Anderson Mark
Published: May 2019

Early Christians adopted a numeric week from Judaism, but wherever their communities spread they encountered the planetary week invented by Hellenistic astrologers. Greek Christians replaced that week with their own in the fourth century C.E., but Latin Christians were only partly successful in doing the same. A timeline for the Christianization of the planetary week between the third and seventh centuries is provided by a database of Greek and Latin documents dated by both an annual calendar and one of the two weeks. The documentary evidence is supplemented by analyses of literary and legal references to the two weeks from the same period. These show that Greek authors were accommodating toward the planetary week while Latin authors were often hostile. Latin hostility was engendered by the presence of non-Christian social practices tied to the planetary week that threatened Christian religious identity. These practices included worship of the planetary deities, rest on the Day of Jupiter rather than on the Lord's Day, and the reservation of activities like marriage, travel, and personal grooming to certain planetary weekdays. A brief survey of the spread of the two weeks throughout much of Afro-Eurasia contextualizes the changes occurring in the Mediterranean and shows that the Latin conflict was unique. That conflict was likely a product of the relatively slow pace of Christianization in some parts of Western Europe and the integration of the planetary week into the Roman calendar to produce a coherent cycle of pagan time which was difficult to replace.
Sequencing of a Wild Apple (Malus baccata) Genome Unravels the Differences Between Cultivated and Wild Apple Species Regarding Disease Resistance and Cold Tolerance
Authors: Chen Xilong , Li Shiming , Zhang Dong , Han Mingyu , Jin Xin , Zhao Caipin , Wang Songbo , Xing Libo , Ma Juanjuan , Ji Jingjing , An Na
Published: May 2019

Malus baccata is one of four wild apple species that can hybridize with the cultivated apple species (Malus domestica). It is widely used in high-latitude apple-producing areas as a rootstock and breeding resource because of its disease resistance, and cold tolerance. A lack of a reference genome has limited the application of M. baccata for apple breeding. We present a draft reference genome for M. baccata. The assembled sequence consisting of 665 Mb, with a scaffold N50 value of 452 kb, included transposable elements (413 Mb) and 46,114 high-quality protein-coding genes. According to a genetic map derived from 390 sibling lines, 72% of the assembly and 85% of the putative genes were anchored to 17 linkage groups. Many of the M. baccata genes under positive selection pressure were associated with plant–pathogen interaction pathways. We identified 2,345 Transcription factor-encoding genes in 58 families in the M. baccata genome. Genes related to disease defense and cold tolerance were also identified. A total of 462 putative nucleotide-binding site (NBS)-leucine-rich-repeat (LRR) genes, 177 Receptor-like kinase (RLK) and 51 receptor-like proteins (RLP) genes were identified in this genome assembly. The M. baccata genome contained 3978 cold-regulated genes, and 50% of these gene promoter containing DREB motif which can be induced by CBF gene. We herein present the first M. baccata genome assembly, which may be useful for exploring genetic variations in diverse apple germplasm, and for facilitating marker-assisted breeding of new apple cultivars exhibiting resistance to disease and cold stress.
Quantifying Microsatellite Mutation Rates from Intestinal Stem Cell Dynamics in Msh2-Deficient Murine Epithelium
Authors: Christopher Joseph , Thorsen Ann-Sofie , Abujudeh Sam , Lourenço Filipe C , Kemp Richard , Potter Paul K , Morrissey Edward , Hazelwood Lee , Winton Douglas J
Published: May 2019

Microsatellite sequences have an enhanced susceptibility to mutation, and can act as sentinels indicating elevated mutation rates and increased risk of cancer. The probability of mutant fixation within the intestinal epithelium is dictated by a combination of stem cell dynamics and mutation rate. Here, we exploit this relationship to infer microsatellite mutation rates. First a sensitive, multiplexed, and quantitative method for detecting somatic changes in microsatellite length was developed that allowed the parallel detection of mutant [CA]n sequences from hundreds of low-input tissue samples at up to 14 loci. The method was applied to colonic crypts in Mus musculus, and enabled detection of mutant subclones down to 20% of the cellularity of the crypt (∼50 of 250 cells). By quantifying age-related increases in clone frequencies for multiple loci, microsatellite mutation rates in wild-type and Msh2-deficient epithelium were established. An average 388-fold increase in mutation per mitosis rate was observed in Msh2-deficient epithelium (2.4 × 10−2) compared to wild-type epithelium (6.2 × 10−5).
Robo-Advisory: The New Paradigm in Asset Management or a Millennial Fad?
Authors: Awuni Mathias
Published: May 2019

The extent to which technology has penetrated every aspect of our lives cannot be overemphasized. Finance and technology are inching closer to convergence by the day; while older investors may be reluctant to have robots doing their investing for them, many millennials are jumping unto the new asset management bandwagon. Thus, allowing algorithms to predict and recommend investment plans and automatically balance portfolios for them based on information such as age, risk tolerance level, investment goals, etc. Robo-advisory software programs periodically buy and sell securities to keep the mix matched to investors’ risk tolerance. This study shows that the emerging issues around fintech only serve to signal that the asset management industry is set for a massive overhaul as more investors seek to substitute the human asset manager for robots and algorithms. Personal finance management especially for low net worth individuals is in a nascent stage of undergoing a complete revolution as these technologies continue to develop. Adventurous investors are encouraged to try robo-advisory services. Although robo-advisory is still at a very rudimentary level especially in developing countries, asset management companies need to recognize the impending disruption and use it to their advantage or risk being smothered by the technology.
Comparison of multicopy pro-microbial transglutaminase encoded gene expression in Pichia pastoris
Authors: Özçelik A. Türkanoğlu
Published: May 2019

Transglutaminases catalyse the formation of an isopeptide bond between the group of γ-carboxamides of glutamine residues and primary amine groups of proteins. It is widely used in different food industries in dairy, meat, and bakery products. In this work, the effect of the copy number of gene expression cassette on the extracellular production of pro-MTGase under the GAP promoter in Pichia pastoris was elucidated. Expression vector carrying the Streptomyces mobaraensis pro-MTGase encoded gene was constructed and transformed into the P. pastoris X33. The production of pro-MTGase in single copy and three copies expression cassettes containing clones were compared under the same fermentation conditions. More than 30% enzyme activity was obtained from single copy expression cassette containing clone compared to three copies expression cassettes containing clone. Besides, the amount of the enzyme produced per cell was found to be 24% higher in the fermentation broth of single copy expression cassette containing clone. As a conclusion, there is an inverse correlation between the extracellular production of pro-MTGase and the copy number of gene expression cassette.
Criação e implementação de um banco de dados cirúrgico totalmente informatizado baseado nas ferramentas Google: experiência inicial em cirurgia cardiovascular.
Authors: Tagliari Ana Paula , da-Silveira Lucas Molinari Veloso , de-Souza Anderson Castro , Gib Marcelo Curcio , de-Freitas Tanara Martins , Martins Cristiano Blaya , Wender Orlando Carlos Belmonte , Cavazzola Leandro Totti
Published: May 2019

RESUMO Objetivo: descrever de maneira prática e detalhada a criação de uma plataforma totalmente informatizada para coleta, armazenamento e análise de dados, inicialmente proposta para cirurgia cardiovascular, com interfaces reprodutíveis e aplicáveis a outras especialidades cirúrgicas, bem como, apresentar a experiência inicial de trabalho com esta ferramenta e os resultados preliminares obtidos após sua implementação em um hospital universitário terciário brasileiro. Métodos: a plataforma foi desenvolvida com base nas ferramentas Google, gratuitas, de fácil utilização e amplamente acessíveis. Desde o início desta iniciativa, em maio de 2015, até a análise preliminar, em fevereiro de 2017, dados de 271 pacientes, consecutivos, submetidos à cirurgia cardiovascular foram prospectivamente recordados e analisados. Resultados: a iniciativa foi implementada com pleno sucesso, com 100% dos pacientes incluídos e sem perda de qualquer variável em um banco de dados composto por mais de 500 variáveis. As complicações pós-operatórias imediatas mais frequentes foram: fibrilação atrial (22,5%), broncopneumonia (10,7%), delirium (10,3%), insuficiência renal aguda (10%), acidente vascular encefálico (5%) e morte (7%). Comparando-se as taxas de mortalidade no primeiro e segundo anos da iniciativa, uma redução de 10,8% para 4% (p=0,042), respectivamente, foi evidenciada. Conclusão: a nova proposta de coleta e armazenamento de dados apresentada neste trabalho mostrou-se plenamente factível e efetiva. Por serem gratuitas, de fácil manuseio e universalmente acessíveis, estas ferramentas podem ser úteis a outras especialidades cirúrgicas que desejem desenvolver métodos de avaliação de sucesso e complicações pós-operatórios, bem como, programas de melhoria de qualidade.
Influência do posicionamento do retalho linfonodal vascularizado na resposta ao tratamento cirúrgico do linfedema secundário ao câncer de mama.
Authors: Montag Eduardo , Okada Alberto Yoshikazu , Arruda Eduardo Gustavo Pires , Fonseca Alexandre Siqueira , Bromley Miluska , Munhoz Alexandre Mendonça , Busnardo Fábio Freitas , Gemperli Rolf
Published: May 2019

RESUMO Objetivo: analisar a experiência terapêutica inicial do transplante de linfonodos vascularizados em pacientes portadoras de linfedema de membros superiores secundário ao tratamento do câncer de mama e verificar se o posicionamento do transplante influencia o resultado cirúrgico. Métodos: ensaio prospectivo, comparativo, de duas modalidades terapêuticas em 24 pacientes portadoras de linfedema de membro superior após tratamento de câncer mamário, classificados como graus 2 e 3, segundo a Sociedade Internacional de Linfedema. Os dois tipos de procedimentos realizados foram: 1) reconstrução total da mama com retalho de perfurante da artéria epigástrica inferior (DIEP- deep inferior epigastric perforator flap) associado ao retalho linfonodal, em pacientes sem reconstrução mamária prévia ou com histórico de perda da reconstrução (posicionamento axilar); 2) retalho linfonodal inguinal isolado foi realizado em pacientes com reconstrução mamária finalizada ou sem o desejo de realizar a reconstrução da mama (posicionamento no punho). Resultados: a porcentagem de redução do volume do membro afetado foi de 20,1% (p=0,0370). O número de episódios infecciosos (celulites) também sofreu redução significativa, de 41% no período pré-operatório para 12,5% no pós-operatório (p=0,004). Não foram observadas diferenças entre os grupos proximal e distal. Conclusão: o transplante de linfonodos afetou positivamente a evolução pós-operatória de pacientes portadoras de linfedema secundário ao câncer de mama. Não foram observadas diferenças em relação ao posicionamento do retalho.
ARTIGO - POLÍTICA, EDUCAÇÃO E ATUALIDADE DO PENSAMENTO FREIRIANO
Authors: HADDAD SÉRGIO
Published: May 2019

RESUMO: Este texto é produto da participação do autor no IIº Congresso Internacional Paulo Freire, na mesa de diálogo “Política, educação e atualidade do pensamento freiriano”. Tomando momentos da vida do educador Paulo Freire, o artigo procura mostrar quão orgânica é a relação entre Política e Educação para ele, quão estruturante esta relação é do seu pensamento, e como foi vivenciada ao longo do tempo. É intenção, com isso, demonstrar a perenidade de alguns conceitos e aquilo que foi transitório para Freire para, assim, tomar o seu pensamento na perspectiva de atualizá-lo para a realidade que vivemos hoje. Nessa perspectiva, ao final, são apresentadas algumas das grandes questões contemporâneas que estão colocadas para a humanidade, em particular, para educadores e educadoras, conclamando a atualidade do pensamento freiriano para enfrentá-las.
ARTIGO - FORMAÇÃO DE EDUCADORAS/ES, DIVERSIDADE E COMPROMISSO SOCIAL
Authors: DINIZ-PEREIRA JÚLIO EMÍLIO , SOARES LEÔNCIO JOSÉ GOMES
Published: May 2019

RESUMO: O título deste artigo foi o “tema gerador” central do 2º Congresso Internacional Paulo Freire: O Legado Global. Nele, os autores partem de uma breve análise sobre a racionalidade crítica na formação de educadoras/es como alternativa às chamadas visões técnica e prática na preparação de novas/os profissionais da educação para, em seguida, apresentarem uma discussão mais teórica e conceitual sobre as diferenças entre os termos “formação de professores para a justiça social”, comum em países como os Estados Unidos, e “formação de educadoras/es por meio da transformação social” - a expressão defendida neste texto. A partir daí, eles discutem algumas características que não poderiam faltar em um programa de formação de educadoras/es por meio da transformação social para, no fim, analisar uma experiência concreta de formação orientada por meio da transformação social: a experiência do Movimento dos Trabalhadores Sem Terra, no Brasil, na década de 1990 e início dos anos 2000.
aATÍCULO - LA PEDAGOGIA DEL OPRIMIDO, LA INVESTIGACIÓN EN SALA DE CLASES Y LOS PROFESORES COMO INVESTIGADORES
Authors: WILLIAMSON GUILLERMO , HIDALGO CAROLINA
Published: May 2019

RESUMEN: Hace 50 años Paulo Freire escribió “La Pedagogía del Oprimido”. Realiza, desde su posición histórica del momento, una lectura dialéctica de una contradicción fundamental de la sociedad y la educación: entre opresores y oprimidos, debiendo la educación posicionarse al lado de estos, contribuyendo a la concientización como superación entre denuncia de un mundo injusto y anuncio de un mundo nuevo. Aquí se re-lee dicho libro desde la investigación en sala de clases realizada por profesores co-investigadores junto a los estudiantes. Una investigación realizada en Chile sobre un proceso investigativo en escuelas, liceos y centros de educación de jóvenes y adultos, analiza opiniones en entrevistas, conversatorios y reuniones de docentes que aplican la metodología, debatiendo una cuestión central: a 50 años de la propuesta de Freire ¿la investigación en sala de clases, según profesores que la desarrollan, puede entenderse como una posibilidad de pedagogía liberadora de los oprimidos?
Combinatorial analysis of agronomic characters in soybean
Authors: Rocha Makyslano Rezende da , Hamawaki Osvaldo Toshiyuki , Nogueira Ana Paula Oliveira , Machado Junior Carlos Sebastião , Hamawaki Cristiane Divina Lemes , Hamawaki Raphael Lemes
Published: May 2019

ABSTRACT Evaluation of the genetic potential of parent plants is fundamental for obtaining superior populations with desirable agronomic characters. The aim of this work was to obtain estimates of the general and specific combining abilities of five soybean parents to identify the most promising parents and hybrid combinations for the development of superior genotypes for grain yield. In this study, two groups of soybean cultivars were selected: group I resistance (UFUS 7910 and BR/MG 46 Conquista) and group II (TMG 4182, TMG 4185 and BRSGO Chapadões). The parents were crossedin a 2 x 3 partial diallel scheme, and the experiment was analyzed according to Griffing’s (1956) parent and F1 model and adapted by Geraldi and Miranda Filho (1988). Six hybrid combinations and respective parents were arranged in randomized complete blocks with four repetitions. The following characters were evaluated: number of days for flowering and maturity, height of the plant in flowering and at maturity, number of nodes in flowering and at maturity, number of total pods and grain yield per plant. The BR/MG 46 Conquista, UFUS 7910 and BRSGO Chapadões parents contributed to obtaining productive segregant populations due to additivity for the total number of pods and grain yield per plant. The hybrid combinations BRSGO Chapadões x BR/MG 46 Conquista, BRSGO Chapadões x UFUS 7910 and TMG4182 x BR/MG 46 Conquista are promising for obtaining populations with high productive potential.
Elemental concentration via portable x-ray fluorescence spectrometry: Assessing the impact of water content
Authors: Santana Monna Lysa Teixeira , Carvalho Geila Santos , Guilherme Luiz Roberto Guimarães , Curi Nilton , Ribeiro Bruno Teixeira
Published: May 2019

ABSTRACT Portable X-ray fluorescence (pXRF) analysis can be considered one of the main recent advances for chemical characterization of earth materials. The water content of the samples can affect the pXRF performance. As a novelty, we aimed to establish relationships (linear regression) between the effect of water content on pXRF results and atomic number (Z) of the elements. Three certified reference materials (CRM) were investigated: OREAS 100a, OREAS 101a, and OREAS 101b. These materials were saturated (0.68 g g-1) with distilled water and left to air-dry naturally. During the drying, the elemental concentrations (C) were determined at different water contents using a pXRF spectrometer. For each water content, the ratio Cwet/Cdry was determined and plotted against the water content. The attenuation coefficient (σ) was also determined. High σ values mean more influence of water content upon measurement element concentration. The obtained recovery rates allowed a qualitative determination. The concentration for the most elements reduced linearly with increasing water content. A predictable behavior of the water content on pXRF results as function of atomic number was not found. Elements identified by Lα spectral line with highest Z were more impacted by water content than elements identified by Kα line with lowest Z. Ti, Cr and Fe was not significantly influenced by water content, and Sr was the most impacted. Our findings contribute to decision-making before characterization earth materials via pXRF, obliging the use of dry samples for determination of impacted elements or by using moisture-corrected data.
O inconsciente capitalista a partir de Lacan, Marx e Zizek: consequências para o debate sobre identidade de gênero
Authors: Alves Cândida Beatriz , Pedroza Regina Lúcia Sucupira
Published: May 2019

RESUMO: Este estudo se fundamenta na psicanálise e no materialismo histórico-dialético, segundo o qual os seres humanos se constituem por processo histórico e social. Com base na apropriação por Lacan da obra de Marx, defendemos que o inconsciente é instância histórica e política, constituído em interação com o contexto onde se insere o sujeito. A partir daí, propomos que a divisão sexual do trabalho está inscrita no inconsciente e a identidade de gênero é constituída a partir desse conteúdo simbólico. A tomada de consciência dessa divisão desigual é impedida por uma fantasia ideológica.
O ESTATUTO PSÍQUICO DO DINHEIRO À LUZ DA TEORIA PSICANALÍTICA
Authors: Silva Lillian Nathalie Oliveira da , Henriques Rogério da Silva Paes
Published: May 2019

RESUMO: O artigo investiga o lugar ocupado pelo dinheiro na economia psíquica de um ponto de vista pulsional, com base na psicanálise. Trata-se de pesquisa com metodologia bibliográfica e leitura interpretativa na perspectiva psicanalítica. Conclui-se que o dinheiro se constitui como objeto pulsional com injunção estruturante sobre o psiquismo, instituindo-se como cifra da própria constituição do sujeito.
TESTEMUNHAR - UM MODO DE COMPARTILHAR O TRAUMA
Authors: Antonello Diego Frichs
Published: May 2019

RESUMO: Este artigo tem como propósito trazer uma contribuição à teoria e clínica do trauma. Para isso, iremos apresentar uma interlocução entre a literatura de testemunho e a psicanálise. Nosso objetivo será mostrar como o reconhecimento e o compartilhar do outro, destacados na literatura de testemunho, podem estar ligados à relação primária e constitutiva de nosso eu e do aparato psíquico, que ocorre na relação estabelecida com os cuidadores. A partir disso, poderemos pensar uma questão clínica importante no que diz respeito ao tratamento da neurose traumática.
O INCONSCIENTE E A LÓGICA A PARTIR DE FREUD E LACAN
Authors: Shimabukuro Felipe
Published: May 2019

RESUMO: Nós propomos, neste artigo, uma interpretação da seguinte passagem do Abriss der Psychoanalyse de Freud: “As regras decisivas da lógica não têm validade no inconsciente, pode-se dizer que ele é o reino do ilógico”. Para tanto, procederemos em quatro etapas: 1) Delimitar o conceito de lógica a partir do Organon de Aristóteles. 2) Identificar quais são as regras decisivas da lógica e seu conteúdo. 3) Examinar em que sentido tais regras não possuem validade no inconsciente. 4) Problematizar a tese freudiana de que o inconsciente seria o reino do ilógico. A ideia de que esta tese freudiana é problemática surge de uma tese que Lacan apresenta no seminário XIV sobre a lógica da fantasia, a saber, que o inconsciente “está instalado no campo da lógica e que ele articula proposições”. Esta tese lacaniana nos permitirá chegar à conclusão de que o inconsciente não é totalmente o reino do ilógico.
PARA UMA SUSPENSÃO DO TEMPO ORDINÁRIO - A EXPERIÊNCIA DA FESTA, DO TÉDIO E DO DIVÃ
Authors: MANZI RONALDO FILHO
Published: May 2019

RESUMO: Este ensaio pretende analisar se, em nossos dias, ainda é possível uma suspensão do tempo ordinário. Ou seja, pretende explorar várias dimensões de nossas vidas nas quais o tempo cronológico poderia ou não ser colocado em suspensão; se sim, tal possibilidade nos levaria a experienciar outra forma de temporalidade? Principio a análise desde a perspectiva da experiência nas festas populares - o carnaval, por exemplo, ainda seria um espaço de transgressão (do ordinário ao profano)? Enveredo então por uma reflexão sobre o passatempo e a indústria cultural. E, em um segundo movimento, investigo se poderíamos suspender aquele tempo ordinário em nossa vida privada, como na experiência do tédio. Outras possibilidades visadas são os casos do divã e do amor. Nestes últimos, teríamos realmente uma outra experiência de temporalidade?
A ENUNCIAÇÃO DO ANALISTA COMO (TRADUÇÃO) POÉTICA: UM PERCURSO PELA POESIA CONCRETA PARA ROÇAR LALÍNGUA DA TÉCNICA LACANIANA
Authors: Carvalho Maura Cristina de , Lazzarini Eliana Rigotto
Published: May 2019

RESUMO: Propomos compreender a poética (Aristóteles, Benjamim) como um aspecto da técnica de enunciação psicanalítica. Partindo da postulação lacaniana conhecida como lalíngua, encontra-se um paralelo entre esta língua preconizada por Lacan e o uso da linguagem tal qual ele é feito na composição da poesia concretista. Através da análise literária de dois poemas brasileiros desta escola, somados às narrativas dos próprios poetas sobre o processo criativo que os incutiu, perfaz-se um caminho para a compreensão da assertiva lacaniana de que “o inconsciente é um savoir-faire com lalíngua” como uma consideração sobre o aspecto de criação poética imbuído no enunciado do analista.