bluuebunny/crossref_metadata_2025 huggingface dataset:
Modification and Innovation of Flour-Based Products into Contemporary Pancong Cakes under the MBKM Program of Indo Global Mandiri University Students
Authors: Letari Rahayu Tri , Hertati Lesi , Asharie Asmawati
Published: February 2025

Pancong Cake is a traditional cake originating from Betawi, and the name "Pancong" is derived from the Betawi language. It holds a distinct identity as a traditional Betawi cake. Over time, the Pancong Cake, once only adorned with granulated sugar, now boasts a variety of toppings to stay current with contemporary tastes. Kekinian Pancong Cake is a food establishment with a booth concept that integrates with other modified and innovated traditional food stands. Situated in the Kambang Iwak area of Palembang, South Sumatra, Pancong Kekinian Cake addresses a community service activity. This initiative presents a two-fold solution. The first solution imparts knowledge to Pancong Cake proprietors on how to create a product with the ability to capture a large market through outreach. The second solution provides examples of product strategies as reference material, presented in a relaxed discussion format while enjoying Pancong Cake. The proposed solution involves fostering collaboration between two or more third-party brands (co-branding) to enhance the product image. This, in turn, aims to bolster consumer confidence, potentially resulting in increased profits or added value to products or services resulting from the co-branding. The outcomes of this community service activity will be disseminated in an accredited national journal article. It is hoped that the concept of this activity can be implemented more broadly by Pancong Cake owners to boost sales through effective product strategies.
Determinants of Consumer Behavior on Social Media: An MBKM Student Activity at UIGM Supporting AKWET Pempek SMEs
Authors: Maulana Fakhrezi , Hertati Lesi , Asharie Asmawati
Published: February 2025

Pempek Akwet is a food sector MSME located in 26 Ilir, Palembang. which has been around for approximately 7 to 8 years. As the name implies, Pempek Akwet provides a variety of food products such as pempek, tekwan, gado-gado and others. The purpose of this research was to find out the data method of consumer grouping and the use of social media. The method used is a qualitative method by interviewing the owner of Pempek Akwet. The results of this research are useful for pempek Akwet in understanding consumer behavior so that they can develop appropriate marketing content on social media owned by pempek Akwet. By using organic marketing techniques through social media, specifically Instagram and Whatsapp, Pempek Akwet managed to increase turnover and sales by reaching a wider market and increasing brand awareness in Palembang. The use of social media features such as likes, comments, engagement, and insights has helped Pempek Akwet analyze consumer data and expand content reach, making this research a valuable reference for MSMEs looking to leverage digital marketing for their business growth.
Analysis of Cost Acceleration Using Time Cost Trade Off Method on Wheat Silo and Pellet Silo Phase III Structure Repair Project in Surabaya
Authors: Klau Maria Junita , Sajiyo Sajiyo , Tjendari Hanie Teki
Published: February 2025

The Wheat Silo and Pellet Silo Structural Repair Project Phase III in Surabaya holds great significance in the wheat flour processing industry in Indonesia. PT Indofood Sukses Makmur Tbk Bogasari Surabaya Division, one of the leading wheat flour producers, required structural repairs on both silos to ensure smooth plant operations and final product quality. The project necessitates proper project management to avoid delays and cost overruns, with a budget of Rp. 28,011,000,000 and an estimated duration of 455 calendar days. This study employs the Time Cost Trade-Off (TCTO) method to analyze options for accelerating the project timeline by considering additional costs that may arise. The results showed that by using TCTO, the project completion time can be accelerated to 359 days from 455 days, reducing the duration by 21.10% at an additional cost of Rp. 739,351,125.00 or 2.64% of the project value. With the addition of 1 hour of overtime per day, the cost of accelerating the project is Rp. 413,445,000.00 or 1.48% of the project value, reducing the work time to 391 days or 14.07% of the original schedule.
The New Product Line Strategies in Maintaining the Sustainability of SMEs
Authors: Rodhiah Rodhiah , Clarissa Tara , Valentina Valentina
Published: February 2025

The role of product lines is very important in increasing the growth of SMEs. Through a variety of product lines sold, the problem of existing market saturation can be resolved, the emergence of new product lines can expand existing products. PKM activities aim to help MSMEs understand new product line strategies in running a business and maintaining survival in the future.  The activity is engaged in a culinary business, with the Pempek Lita located in Jambi that provides various kinds of pempek.  In its activities, it faces various problems, especially in carrying out product line strategies, this is due to the lack of knowledge about things related to product lines. For this reason, PKM programmes aim to help improve the business partners' knowledge. The method offered in this activity is online training/socialisation through Zoom meetings. The material to be delivered includes: basic introduction to product lines; product line strategies and others. The results of the activity show that the problems that arise in partner businesses will be resolved, partners can understand the importance of product line strategies for business sustainability.
Strengthening and Facilitating Community Groups of The Dewata Orchid Program to Address Agribusiness Issues of Siamese Tangerines in Air Talas Village, Muara Enim Regency, South Sumatra
Authors: Wilujeng Catherine Wahyuning , Andriani Azahra
Published: February 2025

Since the transmigration program to Air Talas Village, Muara Enim Regency, in 1987, the majority of the community has been cultivating Siamese tangerines (Citrus tangerina). As a result, this village is known as a producer of Siamese tangerines, with an annual production exceeding 300 tons. However, this high production has yet to be matched with good sales, leading to an oversupply and a decline in selling prices. The presence of Citrus Vein Phloem Degeneration (CVPD) disease poses a threat to tangerine production as it can degrade the quality of the fruit. This situation can reduce the income of farmers and women's groups processing Siamese tangerine derivative products. These conditions prompted the authors' team to initiate the Air Talas Tourism Village Agribusiness Program (Anggrek Dewata) to assist the village community in cultivating Siamese tangerines and marketing their processed products. In its second year, Anggrek Dewata is strengthening the beneficiary groups, namely the Tunas Hijau Farmers Group and the Subur Makmur Women's Farmers Group, to address the agribusiness issues of Siamese tangerines.
Evaluation of Drainage System on Public Road in Front of Sidoarjo Religious Court Office Class 1A
Authors: Ernitasari Windya Yunesti , Witjaksana Budi , Patriadi Andi
Published: February 2025

Flooding in front of the Sidoarjo Class 1A Religious Court Office road happens frequently during the rainy season. It disrupts traffic and damages the roads. Flooding is caused by the condition of the drainage channels, which are not performing efficiently due to sediment barriers such as garbage, and some channels appear to be damaged. The existing channels can no longer accommodate the water discharge caused by the rain, thus water overflows from the drainage channels, flooding the main road. These conditions show the importance of handling drainage problems. One of the ways that can be taken is to evaluate the capacity of existing drainage channels.In this study, the evaluation of drainage channel capacity was carried out using the Hydrological and Hydraulics Analysis method. The purpose of this study was to determine the amount of drainage channel storage capacity required to resolve flooding. The results of this study showed that the design rainfall obtained based on the Log Pearson Type III method was 116.583 mm/day with a 5-year return period. The design flood discharge in channel T1 was 0.717 m3/second, while the capacity of the existing tertiary channel T1 = 0.009 m3/second, so the discharge that overflows from the existing channel T1 was 0.708 m3/second. The capacity of the secondary channel S1 was 2.604 m3 /second, whereas the capacity of the primary channel P1 was 43.043 m3 /second. The capacity of the T1 channel was known to be unable to accommodate the design flood discharge. Therefore, alternative treatments were required to ensure that the T1 channel could accommodate the designed flood discharge.
Simba Kuat: Implementation of an Environmental Cadre Program from School to Village Level in Keluang Village, Tunkal Ilir, South Sumatra
Authors: Aprillia Eka , Hendrawan Wawan , Abistya Risang Raditya
Published: February 2025

Since its inception in 2021 at SMP Negeri 2 Tungkal Ilir, the Synergy Towards a Healthy and Civilized School (Simba Sehati) program has successfully produced environmental cadres through the concept of an environmentally friendly school. Students have become familiar with and skilled in applying Clean and Healthy Living Behavior (PHBS), environmental conservation efforts, and organic farming. The skills acquired include waste bank management, maggot cultivation from organic waste, wastewater treatment, biopore hole creation, hydroponic cultivation of Family Medicinal Plants (TOGA), fish farming in buckets ("budidamber"), and public health. This year, the Simba Sehati program is being implemented at the village level under the Synergy of Ramba Field Towards a Clean and Healthy Keluang (Simba Kuat) program, with the village community as the beneficiaries. In its development, the Simba Kuat program involves environmental cadres actively transferring knowledge to community groups. The program aims to improve the health and environmental quality of Keluang Village and create opportunities for additional income for the residents.
Training on Customer Driven Value Marketing in Improving Customer Satisfaction on SME Products
Authors: Hidayah Nur , Christopher Darrell , Jonathan Darren
Published: February 2025

Customer driven value is a marketing approach that takes social and environmental responsibility into account, while meeting consumer needs and business interests. It also emphasizes the importance of caring for and increasing the capacity of future generations to meet their needs. The activity partner is engaged in a culinary business located on the east ring road, Mulyo 2 RT 28 Kel Talang Bakung, Paal Merah Jambi. In its activities, it faces problems, especially in terms of customer driven value. This activity aims to support partners in increasing understanding of marketing based on the value that must be provided to customers, so that partners can increase competitive advantage. The method of this activity focuses on customer driven value, especially in terms of how business owners interact with the environment and related interests. This strategy also assists partners in efforts to increase the value of products aimed at customers by providing more experiences that can increase customer satisfaction. The activity method is carried out through offline training through the zoom application. This activity expects partners to increase value to customers in marketing their products. The results of the activity show that the implementation of the training went smoothly, the partners enthusiastically accepted the material presented by the PKM leader.
Socialization of Organic Waste Processing Using Composter Drum at SMAN 67 Jakarta
Authors: Sungkono Sungkono , Bhirawa W. Tedja , Arianto Basuki , Moektiwibowo Hari , Mayori Ersha
Published: February 2025

Waste is a form of problem that needs to be managed and carried out in an integrated manner from upstream to downstream so as not to cause environmental pollution. As part of the AdiwiyataSchool, SMAN 67 Jakarta has several working groups (pokja) including the Wast e Pokja, CompostPokja, and Hydroponic Pokja. However, the available solid compost fertilizer production facilities have not been optimally utilized and the working groups that are run have not run according totheir goals. The aim of this PKM activity is to educate students on the significance of converting waste into useful products, enhance the skills of teachers and students in making compost fertilizers, and support the Adiwiyata School program at SMAN 67 Jakarta. The target of thisactivity is the teachers and students of SMAN 67 Jakarta, especially those who are members of theCompost Pokja. The PKM implementation method consists of four stages: (1) Survey and analysis of partner needs, (2) Lecture-based material delivery, (3) Hands-on practice in processing organic waste into solid and liquid compost fertilizers, and (4) Evaluation based on participants' understanding of the material. The results of this PKM activity are as follows: (1) Participants' knowledge of waste processing into higher-value derivative products improved, as evidenced by an increase in pre-test and post-test scores from 79.45 to 85.04; (2) Participants developed skills in producing solid and liquid compost fertilizer for integration into the work program; (3) A composter drum was donated to SMAN 67 Jakarta to support its Adiwiyata School program.
Development Strategy, Competitiveness, and Performance Among Animation Production Students In Chinese Higher Vocational Colleges
Authors: Wenjimi Zhou
Published: February 2025

This descriptive study investigated the development strategy, competitiveness, and performance among animation production majors in Chinese vocational colleges. Specifically, it described the profile of the respondents in terms of sex, grade level, and home location; determined the respondents’ development strategy; identified the respondents’ competitiveness; assessed the respondents’ performance; tested the differences in responses when grouped according to profile; tested the relationships among development strategy, competitiveness, and performance; and proposed a student development program in vocational colleges.
The participants were 500 animation production majors representing a diverse range of institutions and academic backgrounds. According to the study's findings, the majority of the respondents were male in their senior year and were from the suburbs. Additionally, the survey results indicated that most of the respondents concurred with the indicators of development strategy related to long-term goals, short-term plans, personal interests, and professional development, as indicated by the survey results. Furthermore, a substantial number of respondents agreed with the competitiveness indicators concerning industry trends, market demand, and policy support.
A clear majority of the respondents corroborated with the performance indicators in the areas of communication competence, teamwork, innovative thinking, and technical skills as shown in the results. As to the relationship between development strategy, competitiveness, and performance, there were highly significant relationships on all indicators which meant that the better the development strategy, the better the competitiveness and performance.
Pendampingan Perempuan Penyintas Kekerasan Seksual dengan Disabilitas Psikososial
Authors: Shanti Theresia Indira , Yorza Chika Aslia
Published: February 2025

ABSTRAK
Lebih dari 70% kekerasan seksual terjadi dalam relasi dekat antara korban dan pelaku. Ketidaktahuan mengenai bentuk kekerasan seksual dan kesulitan pengelolaan kebutuhan psikologis seringkali membuat individu rentan dimanipulasi diantaranya oleh relasi kuasa dan sexual grooming sehingga menjadi korban kekerasan seksual, apalagi bila relasi ini melibatkan hubungan romantis. Studi ini merupakan studi kasus, bertujuan menggambarkan isu-isu dalam pendampingan perempuan penyintas kekerasan seksual dengan disabilitas psikososial.  Responden terdiri dari dua orang, pernah alami kekerasan seksual dalam hubungan romantis. Kekerasan seksual yang dialami berkontribusi terhadap berkembangnya disabilitas psikososial pada dirinya, dan kemudian disabilitas psikososial ini menyumbang pada kerentanannya untuk kembali alami kekerasan seksual pada dirinya. Tema yang muncul dalam pendampingan adalah 1) Kerentanan korban yang membuat mereka terjebak dalam manipulasi; 2) Stigmatisasi masyarakat; 3) Ketidakpercayaan pada sistem terkait institutional betrayal; 4) Pemberdayaan.  Rekomendasi dikategorikan pada pencegahan dan penanganan, baik untuk penyintas, keluarga, pendamping, dan masyarakat, dengan fokus untuk pemberdayaan penyintas, dan penelitian berikutnya.
Pengaruh Strategi Koping terhadap Intensi Kambuh pada Penyalahguna Narkoba: Peran Moderasi Sikap, Norma dan Kontrol Perilaku
Authors: Lubis Rahmi , Fahlika Vitari
Published: February 2025

Drug abuse is an unlawful act that causes detrimental effects. Abusers need rehabilitation to overcome these effects and prevent relapse. However, rehabilitation programs do not guarantee that individuals will completely stop abusing drugs. One of the factors that influences the intention to relapse is coping strategies. On the other hand, attitudes, norms, and behavioral control regarding drug abuse determine the relapse intention. This research examines the role of attitudes, norms, and behavioral control in assessing the influence of coping strategies on relapse intentions. A total of 60 residents who had relapsed at least once and were undergoing rehabilitation for at least 2 months at the North Sumatra BNN were involved in this research. The research tools are coping strategies and relapse intention scales, tested for validity and reliability. Research data was analyzed using a moderation test with Jamovi software version 2.0. The results show that coping strategies does not influence relapse intentions directly. Attitudes towards drug abuse, subjective norms, and behavioral control significantly increase the negative influence of coping strategies on relapse intentions. Residents who believe drugs are detrimental, feel the environment prohibits abusing them, believe there is little chance of abusing them, and have adaptive coping skills will have a low intention to relapse. The results are advantageous in relapse prevention programs for abusers so that they focus more on cultivating attitudes, norms, and self-efficacy that support abstinence decisions
Smart Model Bus Stop for Special Needy Aged Individuals
Authors: - Dr.S.Rajeswari , - Ms. L. Priyadharshini
Published: February 2025

ABSTRACT: Countries in the Asia region have hot weather around 2–6 months of the year, which might lead to uncomfortable conditions for elder, physically challenged public commuters. Technical teams have made some major enhancements to existing bus stops as Model bus stop by installing Updated version of smart boards, This paper proposes a smart IoT-based environmentally - friendly enhanced design for existing bus stop services in our cities. The microcontroller transmits the sensor readings to a real-time database hosted in the cloud and incorporates a mobile app that notifies operators or maintenance personnel in the case of abnormal readings or breakdowns. Give systematic information to the bus stop smart display board. The mobile app encompasses a map interface enabling operators to remotely monitor the conditions of bus stops such as the temperature, humidity, estimated occupancy, and space availability. In addition to presenting the system’s architecture and detailed design, a system prototype is built to test and validate the proposed solution.
Complete Truth and Fuzzy Genres: Reading Karl Ove Knausgaard’s My Struggle
Authors: Jensen George H.
Published: February 2025

Traditionally, nonfiction is defined as a genre that tells stories which actually happened; it is a product of memory. In contrast, fiction is, in part or whole, a fabrication, a product of imagination. We seem to accept these distinctions even though, as sophisticated readers, we know the simple dichotomy often dissolves, and we are often skeptical about explicit contracts with the reader. In this essay, I will explore the importance of the truth claims made in nonfiction. While we acknowledge narrative truth and metaphorical truth, there is still something important about ‘getting the facts right.’ Instead of defining nonfiction as the genre that deals with what is true, we could say that nonfiction is the genre that deals with the difficulty of sorting out what we know from what we don’t know and from what we thought we knew. If works of nonfiction often point to the difficulty of establishing the truth, then maybe this murkiness of truth should be considered part of the genre, maybe even foundational to it.
The Impact of Fintech-Based Crowd funding on Small and Medium Enterprises (SMEs) in Islamic Economies
Authors: Rehman Muhammad Abdul , Ullah Asad , Shaikh Suman , Abbas Moeen , Shah Faisal Nadeem
Published: February 2025

Fintech revolution in the financial services industry has significantly changed the way SME financing works, mainly for the Islamic countries where Shariah compliance is compulsory. This is a research on the influence of fintech-based crowdfunding on SMEs within the Islamic finance industry to demonstrate if indeed it is a better bet than conventional banking. The study uses both primary and secondary methods, involving 200 SMEs from Malaysia, Indonesia, Saudi Arabia and UAE based on survey and focus group discussion, collected data is also supplemented from the fintech platforms and the reports. The analysis shows that FINTECH crowdfunding improves SME funding access, providing an average revenue increase of 15.2 percent and employment increase of 10.4 percent affirming prior studies that has demonstrated the Islamic FINTECH will have ability to spur entrepreneurship (Rashid et al., 2022). But there are: regulations, which received the mean score of 4.3, and Shariah compliance issues, which received the score of 5.5, thus being the main obstacles to SMEs financial stability and, therefore, to their growth. According to prior observational studies, Islamic fintech enables a shariah-compliant method to the interest-based conventional funding, although such funding’s efficiency relies upon the legal consistency, investor trust, and technology advancement. This paper sheds light on the necessity of improving investor protection procedures, the increased transparency regarding the platforms, and the establishment of unified regulations for Islamic fintech crowdfunding. These insights advance knowledge on fintech solutions in Islamic finance and offer practical implications to the stakeholders in the fintech start-ups, regulators, and SMEs that seek to enhance access to financial services in Islamic countries. Further research on the effects of fintech crowdfunding on the sustainability of SMEs and how to address the regulatory and operational challenges affecting Shariah-compliant crowdfunding platforms should be conducted.
The Impact of Academic Stress on the Mental Health and Well-Being of Higher Education’s Female Students
Authors: Imran Muhammad , Sultan Asif , Imran Ali , Iqbal Mateen
Published: February 2025

At the University Sargodha, a multicultural academic community, female students make efforts trying to achieve their academic goals without neglecting societal expectations. This poses a significant threat to their mental well-being which is quite alarming. At the university, the researchers examined the academic stress and mental health of 154 female students, initially gauging the level of anxiety and depression. The study incorporated SPSS and DASS-21 scales to measure academic stress and depression and anxiety. The study obtained a moderate but significant correlational value between stress and mental health. Using role strain theory, this study attempts to identify some at risk populations, such as ‘fresher’, students from rural areas, or those coming from poor socio economic backgrounds. The lack of discussion on the mental health impacts of stress in particular in the context of parenting creates additional pressures and makes matters worse. In Sargodha, academic stress is considerably detrimental to the psychological wellbeing of female students. It is therefore suggested that these young females are taught coping strategies such as time management, relaxation, stress alleviation techniques, support seeking, and other culturally appropriate ways of dealing with stress.
Genomic and structural insights into Jyvaskylavirus, the first giant virus isolated from Finland
Authors: de Freitas Almeida Gabriel Magno , Arriaga Iker , de Azevedo Bruna Luiza , Leppänen Miika , Abrahao Jonatas Santos , Andreani Julien , Zabeo Davide , Ravantti Janne , Abrescia Nicola GA , Sundberg Lotta-Riina
Published: February 2025

Giant viruses of protists are a diverse and likely ubiquitous group of organisms. Here, we describe Jyvaskylavirus, the first giant virus isolated from Finland. This clade B marseillevirus was found in
Acanthamoeba castellanii
from a composting soil sample in Jyväskylä, Central Finland. Its genome shares similarities with other marseilleviruses, including conserved Nucleocytoviricota genes, histone-like genes, and three unique ORFans. Helium ion microscopy and electron microscopy of infected cells unraveled stages of the Jyvaskylavirus lifecycle, such as single virions attached to the cell membrane, likely triggering endocytosis, and virus-rich vesicle formation within the cytoplasm. We also reconstructed the Jyvaskylavirus particle to 6.3 Å resolution using cryo-EM. The ∼2,500 Å diameter virion displays structural similarities to other
Marseilleviridae
giant viruses. The capsid comprises of 9,240 copies of the major capsid protein ORF184, which possesses a double jellyroll fold arranged in trimers forming pseudo-hexameric capsomers. Below the capsid shell, the internal membrane vesicle encloses the genome. Through cross-structural and -sequence comparisons with other
Marseilleviridae
using AI-based software in model building and prediction, we elucidated ORF142 as the penton protein, which plugs the twelve vertices of the capsid. Five additional ORFs were identified, with models predicted and fitted into densities that either cap the capsomers externally or stabilize them internally.The isolation of Jyvaskylavirus, along with other virus-like particles, not only suggests that these viruses may be widespread in the boreal environment but also provide insights extendable to other marseilleviruses, addressing the previously lacking structural data on minor capsid proteins.
Identification of nonsense-mediated decay inhibitors that alter the tumor immune landscape
Authors: Cook Ashley L , Sur Surojit , Dobbyn Laura , Watson Evangeline , Cohen Joshua D , Ptak Blair , Lee Bum Seok , Paul Suman , Hsiue Emily , Popoli Maria , Vogelstein Bert , Papadopoulos Nickolas , Bettegowda Chetan , Gabrielson Kathy , Zhou Shibin , Kinzler Kenneth W , Wyhs Nicolas
Published: February 2025

Despite exciting developments in cancer immunotherapy, its broad application is limited by the paucity of targetable antigens on the tumor cell surface. As an intrinsic cellular pathway, nonsense-mediated decay (NMD) conceals neoantigens through the destruction of the RNA products from genes harboring truncating mutations. We developed and conducted a high-throughput screen, based on the ratiometric analysis of transcripts, to identify critical mediators of NMD in human cells. This screen implicated disruption of kinase SMG1’s phosphorylation of UPF1 as a potential disruptor of NMD. This led us to design a novel SMG1 inhibitor, KVS0001, that elevates the expression of transcripts and proteins resulting from human and murine truncating mutations in vitro and murine cells in vivo. Most importantly, KVS0001 concomitantly increased the presentation of immune-targetable human leukocyte antigens (HLA) class I-associated peptides from NMD-downregulated proteins on the surface of human cancer cells. KVS0001 provides new opportunities for studying NMD and the diseases in which NMD plays a role, including cancer and inherited diseases.
2-oxoglutarate triggers assembly of active dodecameric Methanosarcina mazei glutamine synthetase
Authors: Herdering Eva , Reif-Trauttmansdorff Tristan , Kumar Anuj , Habenicht Tim , Hochberg Georg , Bohn Stefan , Schuller Jan , Schmitz Ruth A
Published: February 2025

Glutamine synthetases (GS) are central enzymes essential for the nitrogen metabolism across all domains of life. Consequently, they have been extensively studied for more than half a century. Based on the ATP dependent ammonium assimilation generating glutamine, GS expression and activity are strictly regulated in all organisms. In the methanogenic archaeon
Methanosarcina mazei
, it has been shown that the metabolite 2-oxoglutarate (2-OG) directly induces the GS activity. Besides, modulation of the activity by interaction with small proteins (GlnK
1
and sP26) has been reported. Here, we show that the strong activation of
M. mazei
GS (GlnA
1
) by 2-OG is based on the 2-OG dependent dodecamer assembly of GlnA
1
by using mass photometry (MP) and single particle cryo-electron microscopy (cryo-EM) analysis of purified strep-tagged GlnA
1
. The dodecamer assembly from dimers occurred without any detectable intermediate oligomeric state and was not affected in the presence of GlnK
1
. The 2.39 Å cryo-EM structure of the dodecameric complex in the presence of 12.5 mM 2-OG demonstrated that 2-OG is binding between two monomers. Thereby, 2-OG appears to induce the dodecameric assembly in a cooperative way. Furthermore, the active site is primed by an allosteric interaction cascade caused by 2-OG-binding towards an adaption of an open active state conformation. In the presence of additional glutamine, strong feedback inhibition of GS activity was observed. Since glutamine dependent disassembly of the dodecamer was excluded by MP, feedback inhibition most likely relies on an allosteric binding of glutamine to the catalytic site.Based on our findings, we propose that under nitrogen limitation the induction of
M. mazei
GS into a catalytically active dodecamer is not affected by GlnK
1
and crucially depends on the presence of 2-OG.
A lightweight coal-gangue detection model based on parallel deep residual networks
Authors: Jiang Shexiang , Zhou Xinrui
Published: February 2025

To realize the accurate identification of coal-gangue in the process of underground coal transportation and the low-cost deployment of the model, a lightweight coal-gangue detection model based on the parallel depth residual network, called P-RNet, is proposed. For the problem of images of coal-gangue taken under complex conditions, the feature extraction module (FEM) is designed using decoupling training and inference methods. Furthermore, for the problem of the nearest neighbor interpolation upsampling method being prone to produce mosaic blocks and edge jagged edges, a lightweight upsampling operator is used to optimize the feature fusion module (FFM). Finally, to solve the problem, the stochastic gradient descent algorithm is prone to local suboptimal solutions and saddle point problems in the error function optimization process, numerous experiments are carried out on selecting the initial learning rate, and the Lookahead optimizer is used to optimize parameters during backpropagation. Experimental results show that the proposed model can effectively improve the recognition effect, with a corresponding low deployment cost.
Integrated transcriptome and metabolome analyses reveal the differentially expressed metabolites and genes involved in lipid in olive fruits
Authors: Qu Jipeng , Xu Zhou , Peng Zhengsong , Chen Zhenyong , Chen Tao , Ding Chunbang
Published: February 2025

Background

Olive (Olea europaea L.) oil is well-known commercial product worldwide for its nutritional and therapeutic properties. The molecular mechanisms underlying lipid variations in different olive cultivars remain unclear.

Methods

To investigate the molecular mechanism involved in lipid synthesis and metabolism, untargeted metabolome and RNA-Seq analyses were performed based on two varieties of olive fruits, i.e., Kalinjot (JZ) with low oil content and Coratina (KLD) with high oil content.

Results

Totally, 38 lipid compounds of 375 differentially accumulated metabolites (DAMs) were identified in JZ and KLD fruits, with 24 metabolites showing higher contents in KLD than those in JZ. Integrated transcriptome and metabolome analyses identified 48 differentially expressed genes (DEGs) associated with six lipid DAMs from JZ and KLD fruits. The contents of decanoic acid, sphinganine, and leukotriene D4 in KLD fruits were 2.33, 1.91, and 1.53 times greater than that of JZ fruits, respectively. In particular, two BCCP, one ACC, seven KAR, one EAR, one FATA and one SPT genes were observed involving to the content and quality of lipids in olive fruits. These DEGs were associated with the pathways of fatty acid biosynthesis, arachidonic acid metabolism, and limonene degradation. This study provides a strong theoretical and experimental foundation for further revealing the molecular mechanisms regulating lipid synthesis and metabolism in different olive cultivars.
TaZFP 23, a new Cys2/His2-type zinc-finger protein, is a regulator of wheat (Triticum aestivum L.) growth and abiotic stresses
Authors: Ye Shunxing , Tang Yuzhou
Published: February 2025

Wheat (Triticum aestivum L.) is an important food crop and one of the most important grains in the world. With the global climate change, wheat production is increasingly affected by abiotic stress, among which drought, salinity, and other factors have become the main abiotic stress factors restricting the efficient production of wheat. The C2H2-type zinc finger proteins are a common class of transcription factors in plants that play crucial roles in regulating plant growth and development as well as responses to stresses. In this study, the wheat C2H2-type zinc finger protein transcription factor TaZFP23 was cloned. Its full-length coding sequence was 720 bp encoding 239 amino acids. TaZFP23 is a typical C2H2-type zinc finger protein. It contains two C2H2 zinc finger domains and an EAR motif, without a transmembrane domain. Promoter cis-acting element analysis suggested that TaZFP23 might function in abiotic stress responses and plant hormone signal transduction. Subcellular localization and transcriptional activity assays indicated that TaZFP23 encoded a nuclear protein without self-activation activity. Overexpressing TaZFP23 in Arabidopsis thaliana showed that it negatively regulate d seed germination and plant growth under NaCl, mannitol, and ABA treatments. Additionally, TaZFP23 overexpression under NaCl and drought stress in Arabidopsis resulted in lower expression levels of several stress-related marker genes compared to those in wild-type plants. This research provides a foundation for further elucidating the functions of C2H2-type zinc finger protein genes and offers promising candidate genes for the development of stress-tolerant wheat cultivars.
The Role of Productivity on the Relationship between Strategic Performance and Profit
Authors: Kuhait Amal Abdulhusssain , Algburi Ahmed , Sahib Ehab H.
Published: February 2025

The research aimed to know the extent to which productivity linked to profit contributes to evaluating strategic performance as one of the financial methods of the company (Iraqi Engineering Works). The investigator employed the applied analytical methodology in the practical domain, as assessing the impact of productivity changes on existing profits was used to measure productivity linked to profit (productivity Neutral) (PQ) (for materials and labour) as one of the ways to evaluate changes in productivity for the years (2022-2023). The research concluded that the major role that profit-related productivity plays in improving the strategic performance of the economic unit is by using current inputs that provide a more accurate measurement of profit-related productivity than what is often known using Input prices for the base period to evaluate changes in productivity.
Sustainable Production of Liquid Biofertilizer: Utilizing Spent Mushroom Substrate and Watermelon Peels
Authors: P. U. Mini , Onwukwe C.D. , B. A Ezeonuegbu, , C. M Ikedimma , B. T. Daodu, , H. O. Stanley , C. N. Ariole
Published: February 2025

Biofertilizers are natural fertilizers that contains live biomass. This study demonstrates the production of Liquid biofertilizer through the use of spent mushroom substrate (SMS), and watermelon peels by liquid state fermentation technique. This is a result of contamination and lose of soil fertility faced by the environment and agricultural farm land as a result of incessant use of inorganic fertilizers that are persistent in the soil. The two waste materials of 5379g each were obtained and placed on a 35-litre drum, 27 litre of water was poured on the mixture after washing the watermelon peel and stirred evenly, then allowed to ferment for 3 weeks under anaerobic condition, then filtration of the mixture was done after complete fermentation, and microbiological and physicochemical analysis was carried out on the mixture before and after fermentation. Reduction in the concentration of total nitrogen, phosphorous, magnesium and potassium were observed after fermentation while iron content increased after fermentation. The following bacterial species were identified using 16S rRNA gene amplicons: Pseudomonas aeruginosa, Stenotrophomonas, geniculata, Sphingobacterium daejeonense and Alcaligene faecalis. A Pot experiment was carried out with a planting bag on three set ups in duplicates, (i) seed + liquid biofertilizer application, (ii) seed + chemical fertilizer application (iii) Seed alone. Then planting of bean seed (Phasolous vulgaris L.) and groundnut seed (Arachis hypogea) on each set up after physicochemical analysis of the planting soil. Growth comparison using germination test was done by measuring the shoot length, leaf length, leave number, leaf width, stem girth and number of branches, it was observed that the treatment with liquid biofertilizer application competed favourably with that of chemical fertilizer treatment and the biofertilizer also serves as a bio-control agent as the leaves of the biofertilizer set up was not eaten by caterpillar while that of inorganic (chemical fertilizer) was eaten up.
Unraveling the Black Box: The Nexus between Sustainable HR Management Practices and Sustainable Performance
Authors: Mekhur Hanan Ghanim , Almrshed Sattar Kadim Hachim , Abed Oday Jabbar , Sabti Yousif Mousa , Bisheen Ali Saud
Published: February 2025

In solving the triple-bottom-line concept of sustainability, which encompasses the concerns of the modern world, corporations are increasingly incorporating principles of sustainability into their strategies; yet, this presents challenges for the manufacturing industry with limited resources. Despite the increasing academic acknowledgment of SHRM, the impact of SHRMP on an organization's sustainability performance has not been well investigated. Based on signaling theory, we examine the impact of SHRM on the sustainability performance of the organization. Analysis of survey data from 287 workers in the industrial sector indicated that sustainable practices substantially mediate the relationship between SHRMP, managerial competence, and sustainable performance. We analyze the contributions to theory and practice and delineate prospective research areas.
The alkyl and alk‐1‐enyl glycerols in the liver of rats fed long‐chain alcohols or alkyl glycerols
Authors: Bandi Z.L. , Mangold H.K. , Hølmer G. , Aaes-Jørgensen*** E.
Published: July 2002

Dietary long‐chain alcohols and alkyl glycerols, including polyunsaturated compounds, are incorporated into the alkyl and alk‐1‐enyl moieties of the ionic alkoxylipids of rat liver, whereas polyunsaturated fatty acids are not.
A model for chloroplast thylakoid membranes involving orderly arrangements of negatively charged lipidic particles containing sulphoquinovosyldiacylglycerol
Authors: Sakai William S. , Yamamoto Harry Y. , Miyazaki Tatsuo , Ross James W.
Published: July 2002

Addition of sulphoquinivosyldiacylglycerol (SL) to mixtures of monogalactosyldiacylglycerol (MG) and digalactosyldiacylglycerol (DG) induces the appearance of paracrystalline arrays of 80–100 Å lipidic particles. The hypothesis is presented that SL occupies the position in the leaflet opposite the micelle of MG or the position in the leaflet on the convex side of the bulge or cusp in the corresponding model for lipidic particles, and that these orderly arranged lipidic particles containing negatively charged SL may form part of the basis for the orderly arrangement of other molecules functioning in photosyntesis in the thylakoid membrane.
Induced mannosidosis‐excretion of oligosaccharides by locoweed‐intoxicated sheep
Authors: Sadeh Shoshana , Warren Christopher D. , Daniel Peter F. , Bugge Birgitte , James Lynn F. , Jeanloz Roger W.
Published: July 2002

Daily urine samples were collected from a locoweed‐fed sheep, and the oligosaccharide content examined by thin‐layer and liquid chromatography. An unusual pattern of urine oligosaccharides was observed, which appears to be characteristic of loco intoxication. Changes in the pattern could be correlated with the onset of visible disease, which occurred approximately 5 weeks after the typical urine sugars were first detected. HPLC showed that these sugars consisted of two homologous series of oligosaccharides containing one and two residues of 2‐acetamido‐2‐deoxy‐D‐glucose, respectively.
500 MHz 1H‐NMR study of the N‐terminal N‐trimethylalanine residue of LC‐1 and LC‐2 light chains m rabbit fast skeletal myosin solutions
Authors: Roux-Fromy M. , Cardinaud R.
Published: July 2002

High‐resolution proton NMR at 500MHz has been used to study the N‐trimethyl terminals of LC‐1 and LC‐2 light chains in rabbit fast skeletal myosin solutions. The observed resonance is a sum of two Lorentzians withΔv = 5 ± 1 Hz, δ = 3.23 ppm and Δv = 12 ± l Hz, δ = 3.22ppm, respectively. By selective proteolytic modifications samples lacking either the N‐terminal segment of LC‐1 up to Lys‐17 (papain modification) or the N‐terminal segment of LC‐2 up to Arg‐7 (trypsin modification) were prepared. From the NMR spectra of the modified samples the narrower ‐N+(CH3)3 methyl resonance is shown to originate from LC‐1 and the broader from LC‐2. Thus in solution LC‐1 and LC‐2 N‐terminals do not behave identically and there exists between both terminals no interaction reflected by linewidth or chemical shift variation when either N‐terminal is removed. In intact as well as in modified myosins the number of resonating protons corresponds within experimental error to the expected values. The comparison of the present NMR results with the rates of proteolytic cleavage suggests that in solution these light chains could be folded back along S1 but without impeding the motion of the N‐terminal residues, while in filaments the LC‐1 and LC‐2 light chains would expose their median sensitive part to proteolytic enzymes.
Resistance to alloxan of tumoral insulin‐producing cells
Authors: Sener Abdullah , Malaisse Willy J.
Published: July 2002

Rat pancreatic islets and insulin‐producing cells of the RINm5F line were incubated for 5 min at 7 or 23°C in media containing 3H2O and either L‐[1‐14C]glucose or [2‐14C]alloxan. In the islets the intracellular distribution space of [2‐14C]alloxan represented, at 7 and 23°C respectively, 11.4 ± 1.0 and 25.5 ± 2.3% of the intracellular 3H2O space. In the RINm5F cells, the distribution space of [2‐14C]alloxan failed to be affected by the ambient temperature and represented, after correction for extracellular contamination, no more than 5.2 ± 0.5% of the intracellular 3H2O space. Preincubation for 30 min at 7°C in the presence of alloxan (10 mM) failed to affect subsequent D‐[U‐14C]glucose oxidation in the tumoral cells, whilst causing a 70% inhibition of glucose oxidation in the islets. It is proposed that RINmSF cells are resistant to the cytotoxic action of alloxan, this being attributable, in part at least, to poor uptake of the diabetogenic agent.
Primary structure of elongation factor 2 around the site of ADP‐ribosylation is highly conserved from archaebacteria to eukaryotes
Authors: Gehrmann Renate , Henschen Agnes , Klink Friedrich
Published: July 2002

Elongation factor 2 (EF‐2) from eukaryotes and archaebacteria can be ADP‐ribosylated by diphtheria toxin (DT) [(1977) Annu. Rev. Biochem. 46, 69‐94; (1980) Nature 287, 250‐251]. The primary structure of the ADP‐ribose accepting region in EFs from the archaebacteria Thermoplasma acidophilum, Halobacterium cutirubrum and Methanococcus vannielli was determined in order to elucidate the degree of conservation compared with 4 previously established eukaryotic sequences [(1971) FEBS Lett. 103, 253‐255]. Within a 9‐residue sequence including the site of ADP‐ribosylation 5 positions were found to be occupied by the same amino acid in all the archaebacterial and eukaryotic factors studied. There were more differences among the 3 archaebacterial sequences than among the 4 eukaryotic ones.
Immunological quantitation of tyrosinase from wild‐type and albino mutant mice
Authors: Tamate Hidetoshi B. , Kuzumaki Takejiro , Suzuki Jun-ichi , Ishikawa Kiichi
Published: July 2002

The relationship between gene dosage, enzyme activity, and level of immunologically cross‐reacting material (CRM) was examined in mammalian tyrosinase (EC 1.14.18.1) by rocket immunoelectrophoresis. Skin extracts from mice heterozygous (C/c) and homozygous (c/c) for the albino locus contain 46% and 0% of CRM, respectively, as compared with wild‐type (C/C) animals. Enzyme activity and CRM level were directly proportional in these genotypes, suggesting that the albino locus controls the quantity of tyrosinase produced in melanocytes.
Increase of alcohol dehydrogenase and protein content of liver following chronic ethanol administration
Authors: Buris László , Csabai Gyula , Fodor Mihaly , Varga Mihaly
Published: July 2002

Chronic ethanol consumption
Alcohol tolerance
ADH activity
Protein content
Dolichol alters dynamic and static properties of mouse synaptosomal plasma membranes
Authors: Wood W.G. , Gorka C. , Williamson L.S. , Strong R. , Sun A.Y. , Sun G.Y. , Schroeder F.
Published: July 2002

Dolichols are isoprenologues that are found in almost all tissues and whose biochemical function, aside from dolichol phosphate precursors, is not known. In addition, an understanding of the organizational and dynamic properties of dolichols in biological membranes has not been forthcoming. The purpose of the experiments reported here were to examine the effects of dolichol on the physical properties of mouse synaptic plasma membranes (SPM). Differential polarized phase fluorometry indicated that dolichol both fluidized and regidified SPM. Membrane areas detected by diphenylhexatriene and trans‐parinaric acid were selectively fluidized and rigidified, respectively. It also was found that the spin label, 5‐doxyl stearic acid indicated that dolichol reduced membrane fluidity. These results report for the first time a stuctural effect of dolichol on a biological membrane.
In vitro study of the interaction of the LexA represser and the UvrC protein with a uvrC regulatory region
Authors: Granger-Schnarr Michéle , Schnarr Manfred , van Sluis Cees A.
Published: July 2002

The in vitro interaction of the LexA repressor with a regulatory region of the uvrC gene has been studied by polyacrylamide gel electrophoresis. Although the uvrC promoter region shows some homology with the canonic LexA binding site, no specific binding of the repressor to this DNA sequence could be observed, but only a cooperative nonspecific binding. By the same technique we show that the UvrC protein does not bind specifically to this regulatory DNA sequence either, although the protein is able to bind nonspecifically and cooperatively to the double‐stranded DNA fragment.