bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimal Integration of Battery Systems in Grid-Connected Networks for Reducing Energy Losses and CO2 Emissions
Authors: Grisales-Noreña Luis Fernando , Montoya Oscar Danilo , Perea-Moreno Alberto-Jesus
Published: March 2023

This work addressed the problem regarding the optimal integration of battery systems (BS) in grid-connected networks (GCNs) with the purpose of reducing energy losses and CO2 emissions, for which it formulates a mathematical model that considers the constraints associated with the operation of GCNs in a distributed generation environment that includes BS and variable power generation related to photovoltaic (PV) distributed generation (DG) and demand. As solution strategies, three different master–slave methodologies are employed that are based on sequential programming methods, with the aim to avoid the implementation of commercial software. In the master stage, to solve the problem regarding the location and the type of batteries to be used, parallel-discrete versions of the Montecarlo method (PMC), a genetic algorithm (PDGA), and the search crow algorithm (PDSCA) are employed. In the slave stage, the particle swarm optimization algortihm (PSO) is employed to solve the problem pertaining to the operation of the batteries, using a matrix hourly power flow to assess the impact of each possible solution proposed by the master–slave methodologies on the objective functions and constraints. As a test scenario, a GCN based on the 33-bus test systems is used, which considers the generation, power demand, and CO2 emissions behavior of the city of Medellín (Colombia). Each algorithm is executed 1000 times, with the aim to evaluate the effectiveness of each solution in terms of its quality, standard deviation, and processing times. The simulation results obtained in this work demostrate that PMC/PSO is the master–slave methodology with the best performance in terms of solution quality, repeatability, and processing time.
Optimal Control and Parameters Identification for the Cahn–Hilliard Equations Modeling Tumor Growth
Authors: Kadiri Mostafa , Louaked Mohammed , Trabelsi Saber
Published: March 2023

This paper is dedicated to the setting and analysis of an optimal control problem for a two-phase system composed of two non-linearly coupled Chan–Hilliard-type equations. The model describes the evolution of a tumor cell fraction and a nutrient-rich extracellular water volume fraction. The main objective of this paper is the identification of the system’s physical parameters, such as the viscosities and the proliferation rate, in addition to the controllability of the system’s unknowns. For this purpose, we introduce an adequate cost function to be optimized by analyzing a linearized system, deriving the adjoint system, and defining the optimality condition. Eventually, we provide a numerical simulation example illustrating the theoretical results. Finally, numerical simulations of a tumor growing in two and three dimensions are carried out in order to illustrate the evolution of such a clinical situation and to possibly suggest different treatment strategies.
A New Self-Tuning Deep Neuro-Sliding Mode Control for Multi-Machine Power System Stabilizer
Authors: Gu Chan , Chi Encheng , Guo Chujia , Salah Mostafa M. , Shaker Ahmed
Published: March 2023

In order to increase the accuracy and improve the performance of the power system stabilizer (PSS) controller compared to the methods presented in other studies, this paper presents a new method for tuning sliding mode control (SMC) parameters for a PSS using a deep neural network. This controller requires fast switching which can create unwanted signals. To solve this problem, a boundary layer is used. First, the equations of a multi-machine power system are converted into the standard form of sliding mode control, and then the sliding surfaces are determined with three unknown parameters. Calculating and determining the optimal values (at any moment) for these parameters are fundamental challenges. A deep neural network can overcome this challenge and adjust the control system regularly. In the simulation, a power system with 4 machines and 11 buses is implemented and both phase-to-ground and three-phase errors are applied. The simulation results clearly show the good performance of the proposed method and especially the importance of the deep neural network in the SMC structure compared to other methods.
A PINN Surrogate Modeling Methodology for Steady-State Integrated Thermofluid Systems Modeling
Authors: Laugksch Kristina , Rousseau Pieter , Laubscher Ryno
Published: March 2023

Physics-informed neural networks (PINNs) were developed to overcome the limitations associated with the acquisition of large training data sets that are commonly encountered when using purely data-driven machine learning methods. This paper proposes a PINN surrogate modeling methodology for steady-state integrated thermofluid systems modeling based on the mass, energy, and momentum balance equations, combined with the relevant component characteristics and fluid property relationships. The methodology is applied to two thermofluid systems that encapsulate the important phenomena typically encountered, namely: (i) a heat exchanger network with two different fluid streams and components linked in series and parallel; and (ii) a recuperated closed Brayton cycle with various turbomachines and heat exchangers. The results generated with the PINN models were compared to benchmark solutions generated via conventional, physics-based thermofluid process models. The largest average relative errors are 0.17% and 0.93% for the heat exchanger network and Brayton cycle, respectively. It was shown that the use of a hybrid Adam-TNC optimizer requires between 180 and 690 fewer iterations during the training process, thus providing a significant computational advantage over a pure Adam optimization approach. The resulting PINN models can make predictions 75 to 88 times faster than their respective conventional process models. This highlights the potential for PINN surrogate models as a valuable engineering tool in component and system design and optimization, as well as in real-time simulation for anomaly detection, diagnosis, and forecasting.
Levels of Tetrodotoxins in Spawning Pufferfish, Takifugu alboplumbeus
Authors: Asano Masaki , Ishizaki Chihiro , Tomonou Taiga , Kihara Masato , Ito Masaaki , Yasukawa Shino , Shirai Kyoko , Oyama Hikaru , Izawa Shin , Kawamura Reona , Saito Kanae , Suo Rei , Nakahigashi Ryota , Adachi Masaatsu , Nishikawa Toshio , Sugita Haruo , Itoi Shiro
Published: March 2023

Tetrodotoxin (TTX), also known as pufferfish toxin, is an extremely potent neurotoxin thought to be used as a biological defense compound in organisms bearing it. Although TTX was thought to function as a chemical agent for defense and anti-predation and an attractant for TTX-bearing animals including pufferfish, it has recently been demonstrated that pufferfish were also attracted to 5,6,11-trideoxyTTX, a related compound, rather than TTX alone. In this study, we attempted to estimate the roles of TTXs (TTX and 5,6,11-trideoxyTTX) in the pufferfish, Takifugu alboplumbeus, through examining the location of TTXs in various tissues of spawning pufferfish from Enoshima and Kamogawa, Japan. TTXs levels in the Kamogawa population were higher than those in the Enoshima population, and there was no significant difference in the amount of TTXs between the sexes in either population. Individual differences were greater in females than in males. However, the location of both substances in tissues differed significantly between sexes: male pufferfish accumulated most of their TTX in the skin and liver and most of their 5,6,11-trideoxyTTX in the skin, whereas females accumulated most of their TTX and 5,6,11-trideoxyTTX in the ovaries and skin.
Non-Destructive Testing Inspection for Metal Components Produced Using Wire and Arc Additive Manufacturing
Authors: Serrati Douglas S. M. , Machado Miguel A. , Oliveira J. P. , Santos Telmo G.
Published: March 2023

The wire and arc additive manufacturing (WAAM) process enables the creation and repair of complex structures based on the successive deposition of fed metal in the form of a wire that is fused with an electric arc and then solidifies. The high number of depositions required to create or repair parts increases the likelihood of defect formation. If these are reliably detected during manufacturing, timely correction is possible. However, high temperatures and surface irregularity make inspection difficult. Furthermore, depending on the size, morphology, and location of the defect, the part can be rejected. Recent studies have shown that non-destructive testing (NDT) based on different physical phenomena for the timely, reliable, and customized detection of defects can significantly reduce the rejection rate and allow in-line repair, which consequently reduces waste and rework. This paper presents the latest developments in NDT for WAAM and its limitations and potential.
The Effects of Cold Rolling and Annealing on the Microstructure Evolution of Ordered C-2000 Alloy during Metallic Wire Preparation
Authors: Yuan Liang , Gou Faqiang , Sun Deqiang , Li Zhiqiang , Xue Yunlong
Published: March 2023

When using well-designed multiple-stage heavy-drawn processes, i.e., cold rolling, drawing and cluster drawing to fabricate a metallic wire or fiber in steps, cold rolling and annealing are critical steps due to their effect on the initial microstructure before the heavy-drawn process. Understanding the relationship between microstructure evolution and cold rolling followed by annealing is required for smoothly implementing the heavy-drawn process. In this work, the evolution behavior in terms of the microstructure during cold rolling followed by annealing was investigated in a novel C-2000 alloy that is a promising candidate material for the fabrication of high-performance metallicwire. The investigation encompassed parameters including the grain size, grain boundaries, recrystallization texture, and short-range ordered (SRO) structure. Results show that the grain size distribution of the cold-rolledC-2000 alloy followed by annealing at 900 °C is quite uneven. The low-angle grain boundaries induced by cold rolling are more frequently transformed into the Σ3 twin boundaries during recrystallization. At the initial stage of annealing at 900 °C after cold rolling, the contents of different texture components are significantly different, but the differences tend to decrease with the extension of the annealing time. In addition, cold rolling destroys SRO domains formed during solid solution water quenching, and the destruction of SRO affects the precipitation of the long-range ordered phase during annealing. Incoherent Σ3ic with curved grain boundaries play an important role in the recrystallization of nucleation sites in the process of static recrystallization by nucleation–growth.
Phase Formation Features of Reactor Pressure Vessel Steels with Various Ni and Mn Content under Conditions of Neutron Irradiation at Increased Temperature
Authors: Kuleshova Evgenia , Fedotov Ivan , Maltsev Dmitriy , Fedotova Svetlana , Zhuchkov Georgiy , Potekhin Alexander
Published: March 2023

In this paper the phase formation and mechanical properties of VVER-type reactor pressure vessel (RPV) steels with various Ni (1.57–5.95 wt.%) and Mn (0.03–0.76 wt.%) content after neutron irradiation up to fluences in the range of (53–120) × 1022 n/m2 at 400 °C were studied. The possibility of carbonitride formation under these irradiation conditions is shown. In case of sufficient Ni (>1.5 wt.%) and Mn (>0.3 wt.%) content formation of Ni-Si-Mn precipitates is observed. Their chemical composition is close to G-phase and Γ2-phase and differs from that of radiation-induced precipitates in VVER-1000 RPV steels. This indicates the prerequisites for thermally conditioned mechanism of Ni-Si-Mn precipitates formation and growth at 400 °C enhanced by irradiation. It is also shown that the optimized steel manufacturing technology coupled with an ultralow Mn content (≤0.03 wt.%) in steel with increased up to 5.26 wt.% Ni content facilitates suppressing the Ni-Si-Mn precipitates and carbonitrides formation. This, in turn, reduces the contribution of the hardening embrittlement mechanism and, correspondingly, facilitates high radiation resistance of the steels with ultralow Mn content at the increased irradiation temperature (400 °C).
First-Principles Study of Structural Stability and Mechanical Properties of Ta–W–Hf Alloys
Authors: Ran Xudong , Huang Shuhai , Zhou Shaolan , Lei Wei , Wu Yang , Chen Qiang
Published: March 2023

In order to obtain the effect of W and Hf elements on the mechanical properties of Ta–W–Hf alloys, the structural, mechanical, and electronic properties of Ta–xW–6.25Hf (x = 6.25, 12.50, 18.75, 25.00, 31.25, 50.00) alloys were studied using first-principles calculation based on density functional theory, and the supercell method. The calculated formation enthalpy and elastic constants clarify that Ta–W–Hf alloys have structural and dynamical stability. The formation enthalpy and the cohesive energy decrease with the increase in W content, and the cohesive energy increases when Hf element is added to Ta–W alloy. In addition, bulk modulus (B), shear modulus (G), and Young’s modulus (E) for each of the Ta–xW alloys increase gradually with the increase in W concentration. The B, G, and E of Ta–xW–6.25Hf alloys is lower than that of Ta–xW alloy under the same W content conditions, suggesting that Hf alloying with higher Ta–W concentration becomes softer than the Ta–W alloy. Based on the mechanical characteristic, the B/G and Poisson’s ratio of Ta–W–Hf alloys are higher than those of Ta–W alloys with W content over 25%, the ductility of Ta–W–Hf alloys improves with the addition of Hf, and Hf can reduce the anisotropy of Ta–W–Hf alloys. Furthermore, the electronic density of states shows that alloying W and Hf improves the metallicity of Ta. The results in this work provide the underlying insights needed to guide the design of Ta–W–Hf alloys with excellent mechanical properties.
Electrodeposition of Iron Triad Metal Coatings: Miles to Go
Authors: Lebedeva Olga , Fishgoit Larisa , Knyazev Andrey , Kultin Dmitry , Kustov Leonid
Published: March 2023

The possibilities and future perspectives of electrochemical deposition of bimetallic compositions and alloys containing Fe, Co, Ni, Cr, W, and Mo are reviewed. The synthesis of two- and three-component materials, as well as compositionally more complex alloys, is considered. The method of synthesizing of materials via electrodeposition from solutions containing metal ions and metalloids is one of the most promising approaches because it is fast, cheap, and it is possible to control the composition of the final product with good precision. Corrosion, catalytic and magnetic properties should be distinguished. Due to these properties, the range of applications for these alloys is very wide. The idea of a correlation between the magnetic and catalytic properties of the iron-triad metal alloys is considered. This should lead to a deeper understanding of the interplay of the properties of electrodeposited alloys. In addition to deposition from aqueous (classical) solvents, the advantages and perspectives of electrochemical deposition from ionic liquids (ILs) and deep eutectic solvents (DES) are briefly discussed. The successful use and development of this method of electrodeposition of alloys, which are quite difficult or impossible to synthesize in classical solvents, has been demonstrated and confirmed.
Electrooxidation Performance of a Cotton-Cloth-Derived, Ni-Based, Hollow Microtubular Weave Catalytic Electrode for Methanol and Urea
Authors: Hou Guangya , Wei Jiaxuan , Chen Qiang , Zhang Jianli , Tang Yiping
Published: March 2023

Increasing consumption produces a large amount of cotton textile waste, the conversion of which into porous metals used for energy purposes is of practical value. In this paper, a porous, Ni-based, hollow microtubular weave (Ni-HTW) is obtained from cotton weave by high-temperature carbonization and hydrothermal synthesis with high-temperature reduction. The Ni-based, hollow microtubules in this weave have a diameter of 5–10 μm and a wall thickness of about 1 μm, and every 15 microtubules form a loose bundle with a diameter of 150–200 μm. For improved performance, Ni(OH)2 nanosheets are further electrodeposited on the fibers’ surface of the Ni-HTW to form a nano-Ni(OH)2/Ni-HTW composite electrode with a core–shell heterostructure where Ni is the core and Ni(OH)2 the shell. The combination of hollow microtubule weave morphology and nanosheet structure results in a large specific surface area and abundant active sites, and the composite electrode shows excellent electrocatalytic performance and long-term stability for methanol oxidation (MOR) and urea oxidation (UOR). The current densities can reach 303.1 mA/cm2 and 342.5 mA/cm2 at 0.8 V, and 92.29% (MOR) and 84.41% (UOR) of the pre-cycle current densities can be maintained after 2000 consecutive cycles.
Effect of Al2O3 Inclusions or Mold Flux Particles on Their Surrounding Microstructures of Sliver Defects on the Surface of Automobile Exposed Panel
Authors: Zhang Qing , Li Tingting , Yang Jian
Published: March 2023

The Al2O3 inclusions and mold flux particles are the initial causes of the sliver defects on the surface of automobile exposed panels. During the rolling process, the smashed Al2O3 inclusions or mold flux particles will hinder the growth of recrystallized grains. Compared with mold flux particles, the smashed Al2O3 inclusions have a smaller size, a denser distribution, and a larger number density, so the smashed Al2O3 inclusions have a stronger ability to hinder grain boundary migration. Therefore, the average grain size is small in the following sequence: Al2O3 defect zone with Al2O3 inclusions (Al2O3 DZ with Al2O3), mold flux defect zone with mold flux particles (MFDZ with MFP), mold flux defect zone without mold flux particles (MFDZ without MFP), Al2O3 defect zone without Al2O3 inclusions (Al2O3 DZ without Al2O3), and non-defect zone (NDZ). The influence of particles on the grain orientation of the defect zones results in the microtexture of Al2O3 DZ without Al2O3 is {111}<313>, which is close to the {111}<101> microtextures of NDZ and MFDZ without MFP, while the {001}<114> microtexture on Al2O3 DZ with Al2O3 and the {313}<111> microtexture on MFDZ with MFP are quite different from that of NDZ. Due to differences in the inclusions, orientation, and microtexture of the defect zones and NDZ, dark-gray or bright white sliver defects on the surface of the automobile exposed panel are eventually formed.
Comparison of Plasma Metabolome Response to Diets Enriched in Soybean and Partially-Hydrogenated Soybean Oil in Moderately Hypercholesterolemic Adults-A Pilot Study
Authors: Huang Neil K. , Lichtenstein Alice H. , Matuszek Gregory , Matthan Nirupa R.
Published: March 2023

Partially-hydrogenated fat/trans fatty acid intake has been associated with adverse effects on cardiometabolic risk factors. Comparatively unexplored is the effect of unmodified oil relative to partially-hydrogenated fat on the plasma metabolite profile and lipid-related pathways. To address this gap, we conducted secondary analyses using a subset of samples randomly selected from a controlled dietary intervention trial involving moderately hypercholesterolemic individuals. Participants (N = 10, 63 ± 8 y, BMI, 26.2 ± 4.2 kg/m2, LDL-C, 3.9 ± 0.5 mmol/L) were provided with diets enriched in soybean oil (SO) and partially-hydrogenated soybean oil (PHSO). Plasma metabolite concentrations were determined using an untargeted approach and pathway analysis using LIPIDMAPS. Data were assessed using a volcano plot, receiver operating characteristics curve, partial least square-discrimination analysis and Pearson correlations. Among the known metabolites higher in plasma after the PHSO diet than the SO diet, the majority were phospholipids (53%) and di- and triglycerides (DG/TG, 34%). Pathway analysis indicated upregulation of phosphatidylcholine synthesis from DG and phosphatidylethanolamine. We identified seven metabolites (TG_56:9, TG_54:8, TG_54:7, TG_54:6, TG_48:5, DG_36:5 and benproperine) as potential biomarkers for PHSO intake. These data indicate that TG-related metabolites were the most affected lipid species, and glycerophospholipid biosynthesis was the most active pathway in response to PHSO compared to SO intake.
Additive Manufactured Piezoelectric-Driven Miniature Gripper
Authors: Ferrara-Bello C. Andres , Tecpoyotl-Torres Margarita , Rodriguez-Fuentes S. Fernanda
Published: March 2023

In several cases, it is desirable to have prototypes of low-cost fabrication and adequate performance. In academic laboratories and industries, miniature and microgrippers can be very useful for observations and the analysis of small objects. Piezoelectrically actuated microgrippers, commonly fabricated with aluminum, and with micrometer stroke or displacement, have been considered as Microelectromechanical Systems (MEMS). Recently, additive manufacture using several polymers has also been used for the fabrication of miniature grippers. This work focuses on the design of a piezoelectric-driven miniature gripper, additive manufactured with polylactic acid (PLA), which was modeled using a pseudo rigid body model (PRBM). It was also numerically and experimentally characterized with an acceptable level of approximation. The piezoelectric stack is composed of widely available buzzers. The aperture between the jaws allows it to hold objects with diameters lower than 500 μm, and weights lower than 1.4 g, such as the strands of some plants, salt grains, metal wires, etc. The novelty of this work is given by the miniature gripper’s simple design, as well as the low-cost of the materials and the fabrication process used. In addition, the initial aperture of the jaws can be adjusted, by adhering the metal tips in the required position.
Evaluation of Polydimethylsiloxane (PDMS) as a Substrate for the Realization of Flexible/Wearable Antennas and Sensors
Authors: Sharma Praveen Kumar , Chung Jae-Young
Published: March 2023

To demonstrate that the silicone-based polymer polydimethylsiloxane (PDMS) is suitable as a substrate for flexible/wearable antennae and sensors, an investigation of its various properties was carried out. The substrate was first developed in compliance with the requirements, and then its anisotropy was investigated using an experimental bi-resonator approach. This material exhibited modest but discernible anisotropy, with values of ~6.2/25 % for the dielectric constant and loss tangent, respectively. Its anisotropic behavior was confirmed by a parallel dielectric constant (εpar) ~2.717 and an evaluated perpendicular dielectric constant (εperp) ~2.570—εpar > εperp by 5.7%. Temperature affected PDMS’s dielectric properties. Lastly, the simultaneous impact of bending and anisotropy of the flexible substrate PDMS on the resonance properties of planar structures was also addressed, and these had diametrically opposed effects. PDMS appears to be a good contender as a substrate for flexible/wearable antennae and sensors based on all experimental evaluations conducted for this research.
Assessment of New and Genome-Reduced Pseudomonas Strains Regarding Their Robustness as Chassis in Biotechnological Applications
Authors: Cárdenas Espinosa María José , Schmidgall Tabea , Pohl Jessica , Wagner Georg , Wynands Benedikt , Wierckx Nick , Heipieper Hermann J. , Eberlein Christian
Published: March 2023

Organic olvent-tolerant strains of the Gram-negative bacterial genus Pseudomonas are discussed as potential biocatalysts for the biotechnological production of various chemicals. However, many current strains with the highest tolerance are belonging to the species P. putida and are classified as biosafety level 2 strains, which makes them uninteresting for the biotechnological industry. Therefore, it is necessary to identify other biosafety level 1 Pseudomonas strains with high tolerance towards solvents and other forms of stress, which are suitable for establishing production platforms of biotechnological processes. In order to exploit the native potential of Pseudomonas as a microbial cell factory, the biosafety level 1 strain P. taiwanensis VLB120 and its genome-reduced chassis (GRC) variants as well as the plastic-degrading strain P. capeferrum TDA1 were assessed regarding their tolerance towards different n-alkanols (1-butanol, 1-hexanol, 1-octanol, 1-decanol). Toxicity of the solvents was investigated by their effects on bacterial growth rates given as the EC50 concentrations. Hereby, both toxicities as well as the adaptive responses of P. taiwanensis GRC3 and P. capeferrum TDA1 showed EC50 values up to two-fold higher than those previously detected for P. putida DOT-T1E (biosafety level 2), one of the best described solvent-tolerant bacteria. Furthermore, in two-phase solvent systems, all the evaluated strains were adapted to 1-decanol as a second organic phase (i.e., OD560 was at least 0.5 after 24 h of incubation with 1% (v/v) 1-decanol), which shows the potential use of these strains as platforms for the bio-production of a wide variety of chemicals at industrial level.
Examining the Prevalence and Antibiotic Susceptibility of S. aureus Strains in Hospitals: An Analysis of the pvl Gene and Its Co-Occurrence with Other Virulence Factors
Authors: Vittorakis Eftychios , Vică Mihaela Laura , Zervaki Calina Oana , Vittorakis Evangelos , Maraki Sofia , Mavromanolaki Viktoria Eirini , Schürger Michael Ewald , Neculicioiu Vlad Sever , Papadomanolaki Evangelia , Sinanis Theodoros , Giannoulaki Georgia , Xydaki Evangelia , Kastanakis Serafeim G. , Junie Lia Monica
Published: March 2023

S. aureus is a pathogenic bacterium that causesinfections. Its virulence is due to surface components, proteins, virulence genes, SCCmec, pvl, agr, and SEs, which are low molecular weight superantigens. SEs are usually encoded by mobile genetic elements, and horizontal gene transfer accounts for their widespread presence in S. aureus. This study analyzed the prevalence of MRSA and MSSA strains of S. aureus in two hospitals in Greece between 2020–2022 and their susceptibility to antibiotics. Specimens collected were tested using the VITEK 2 system and the PCR technique to detect SCCmec types, agr types, pvl genes, and sem and seg genes. Antibiotics from various classes were also tested. This study examined the prevalence and resistance of S. aureus strains in hospitals. It found a high prevalence of MRSA and that the MRSA strains were more resistant to antibiotics. The study also identified the genotypes of the S. aureus isolates and the associated antibiotic resistances. This highlights the need for continued surveillance and effective strategies to combat the spread of MRSA in hospitals. This study examined the prevalence of the pvl gene and its co-occurrence with other genes in S. aureus strains, as well as their antibiotic susceptibility. The results showed that 19.15% of the isolates were pvl-positive and 80.85% were pvl-negative. The pvl gene co-existed with other genes, such as the agr and enterotoxin genes. The results could inform treatment strategies for S. aureus infections.
Evaluation of Bacillus paramycoides Strains Isolated from Channa Fish sp. on Growth Performance of Labeo rohita Fingerlings Challenged by Fish Pathogen Aeromonas hydrophila MTCC 12301
Authors: Yousuf Sufiara , Jamal Mamdoh T. , Al-Farawati Radwan Kahalid , Al-Mur Bandar Ahmad , Singh Rahul
Published: March 2023

Probiotics play vital roles in improving growth, survival, and immune responses and inhibit the growth of pathogenic bacteria in freshwater fish. This study was conducted to isolate potential probiotics from Channa punctatus and Channa striatus and to evaluate their effect on Labeo rohita fingerlings. Among the isolates, Bacillus paramycoides PBG9D and BCS10 (1) exhibited antimicrobial activity against the fish pathogen Aeromonas hydrophila. Both strains showed tolerance to acidic and alkaline pH (2, 3, 4, 7, and 9) and bile salts (0.3%) and exhibited strong adhesion capacity. After in-vitro assessment, these strains were evaluated on the growth performances of rohu fingerlings challenged by Aeromonas hydrophila for 4 weeks. The study consisted of six groups, each containing 6 fish. Group (I) was the control, fed a basal diet; group (II) contained a pathogen and was also fed a basal diet; group (III & IV) was given a probiotic supplemented experimental diet; Fourth group (V & VI) contained a pathogen and was given a probiotic supplemented experimental diet. After the 12th day of experiment, rohu fingerlings of pathogen (II) and probiotic + pathogen (V & VI) groups were intraperitoneally injected with 0.1 mL of Aeromonas hydrophila. After 4 weeks, no significant differences in weight gain, weight gain %, and feed conversion ratio were observed in probiotic (III & IV)- fed groups compared to control. However, the specific growth rate was significantly improved in probiotic fed groups compared to other groups. Survival rate and condition factor were significantly similar in all groups. After injection, abnormal swimming, loss of appetite and weight loss were observed in the pathogen (II) group, while no such symptoms were found in the probiotic + pathogen (V & VI)- groups, confirming the effects of probiotics. The overall results of the study revealed that dietary supplementation with Bacillus paramycoides strains could improve the specific growth rate and disease resistance against Aeromonas hydrophila in Labeo rohita.
The 4-α-Glucanotransferase AcbQ Is Involved in Acarbose Modification in Actinoplanes sp. SE50/110
Authors: Nölting Sophia , März Camilla , Jacob Lucas , Persicke Marcus , Schneiker-Bekel Susanne , Kalinowski Jörn
Published: March 2023

The pseudo-tetrasaccharide acarbose, produced by Actinoplanes sp. SE50/110, is a α-glucosidase inhibitor used for treatment of type 2 diabetes patients. In industrial production of acarbose, by-products play a relevant role that complicates the purification of the product and reduce yields. Here, we report that the acarbose 4-α-glucanotransferase AcbQ modifies acarbose and the phosphorylated version acarbose 7-phosphate. Elongated acarviosyl metabolites (α-acarviosyl-(1,4)-maltooligosaccharides) with one to four additional glucose molecules were identified performing in vitro assays with acarbose or acarbose 7-phosphate and short α-1,4-glucans (maltose, maltotriose and maltotetraose). High functional similarities to the 4-α-glucanotransferase MalQ, which is essential in the maltodextrin pathway, are revealed. However, maltotriose is a preferred donor and acarbose and acarbose 7-phosphate, respectively, serve as specific acceptors for AcbQ. This study displays the specific intracellular assembly of longer acarviosyl metabolites catalyzed by AcbQ, indicating that AcbQ is directly involved in the formation of acarbose by-products of Actinoplanes sp. SE50/110.
Screening and Identification of Goat-Milk-Derived Lactic Acid Bacteria with Bacteriocin-like Activity and Probiotic Potentials
Authors: Yang Zhenzhu , Ni Ling , Tian Wei , Chi Hai
Published: March 2023

In the current study, we screened 46 isolates of lactic acid bacteria (LAB) derived from goat milk for bacteriocin producers that can inhibit common foodborne pathogens (Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus). The following three strains that showed antimicrobial activity against all indicators were identified: Enterococcus faecalis DH9003 and DH9012, and Lactococcus lactis DH9011. Their antimicrobial products exhibited typical bacteriocin characteristics, such as heat stability and proteinase nature. The bacteriostatic activity of concentrated bacteriocins produced by these LAB was observed at low concentrations (half-minimum inhibitory concentration [MIC50] and 4MIC50), whereas complete inhibition activity against Listeria monocytogenes was detected at high concentrations (16MIC50) of the two Enterococcus faecalis strains (DH9003 and DH9012). Furthermore, the probiotic potentials of the three strains were investigated and described. The results revealed that none of the strains had hemolytic activity, whereas all: were sensitive to ampicillin (50 mg/mL) and streptomycin sulfate (100 mg/mL); were resistant to bile, artificial simulated intestinal tract, and gastric juice at different pH levels (2.5, 3.0, 3.5); and had β-galactosidase activity. Furthermore, all strains exhibited an auto-aggregating phenotype, with self-aggregation ranging from 30% to 55%. DH9003 and DH9012 co-aggregated well with Listeria monocytogenes and Escherichia coli (52.6% and 63.2%, 68.5% and 57.6%, respectively), whereas DH9011 co-aggregated poorly with Listeria monocytogenes (15.6%) and did not co-aggregate with Escherichia coli. Furthermore, our results showed that all three isolates exhibited strong antibacterial activity, tolerance to bile and simulated gastrointestinal environments, adhesion capability, and safety. Finally, DH9003 was selected and used for gavage in rats. By observing the pathological characteristics of rat intestinal and liver tissue sections, DH9003 showed no harmful effects on the intestine and liver of rats, but rather resulted in a denser and longer intestinal mucosa, as well as improving the intestinal mucosa of rats. Considering their substantial prospective applications, we concluded that these three isolates are potential probiotic candidates.
Expression and Characterization of 3,6-Dihydroxy-picolinic Acid Decarboxylase PicC of Bordetella bronchiseptica RB50
Authors: Yuan Cansheng , Zhao Lingling , Tong Lu , Wang Lin , Ke Zhuang , Yang Ying , He Jian
Published: March 2023

Picolinic acid (PA) is a typical mono-carboxylated pyridine derivative produced by human/animals or microorganisms which could be served as nutrients for bacteria. Most Bordetella strains are pathogens causing pertussis or respiratory disease in humans and/or various animals. Previous studies indicated that Bordetella strains harbor the PA degradation pic gene cluster. However, the degradation of PA by Bordetella strains remains unknown. In this study, a reference strain of genus Bordetella, B. bronchiseptica RB50, was investigated. The organization of pic gene cluster of strain RB50 was found to be similar with that of Alcaligenes faecalis, in which the sequence similarities of each Pic proteins are between 60% to 80% except for PicB2 (47% similarity). The 3,6-dihydroxypicolinic acid (3,6DHPA) decarboxylase gene (BB0271, designated as picCRB50) of strain RB50 was synthesized and over-expressed in E. coli BL21(DE3). The PicCRB50 showed 75% amino acid similarities against known PicC from Alcaligenes faecalis. The purified PicCRB50 can efficiently transform 3,6DHPA to 2,5-dihydroxypyridine. The PicCRB50 exhibits optimal activities at pH 7.0, 35 °C, and the Km and kcat values of PicCRB50 for 3,6DHPA were 20.41 ± 2.60 μM and 7.61 ± 0.53 S−1, respectively. The present study provided new insights into the biodegradation of PA by pathogens of Bordetella spp.
Can Acanthamoeba Harbor Monkeypox Virus?
Authors: Siddiqui Ruqaiyyah , Muhammad Jibran Sualeh , Alharbi Ahmad M. , Alfahemi Hasan , Khan Naveed Ahmed
Published: March 2023

Acanthamoeba is well known to host a variety of microorganisms such as viruses, bacteria, protozoa, and yeast. Given the recent number of cases of monkeypox infection, we speculate that amoebae may be aiding viral transmission to the susceptible hosts. Although there is no confirmatory evidence to suggest that Acanthamoeba is a host to monkeypox (a double-stranded DNA virus), the recent discovery of mimivirus (another double-stranded DNA virus) from Acanthamoeba, suggests that amoebae may shelter monkeypox virus. Furthermore, given the possible spread of monkeypox virus from animals to humans during an earlier outbreak, which came about after patients came in contact with prairie dogs, it is likely that animals may also act as mixing vessel between ubiquitously distributed Acanthamoeba and monkeypox virus, in addition to the environmental habitat that acts as an interface in complex interactions between diverse microorganisms and the host.
Editorial for Special Issue “Composition, Geochronology and Geodynamic Implications of Igneous Rock”
Authors: Khromykh Sergey V.
Published: March 2023

Magmatism is a very important manifestation of the deep activity of the Earth [...]
Tunable Raman Gain in Transparent Nanostructured Glass-Ceramic Based on Ba2NaNb5O15 †
Authors: Pernice Pasquale , Sirleto Luigi , Rossi Manuela , Iodice Mario , Vergara Alessandro , Di Girolamo Rocco , Luciani Giuseppina , Imparato Claudio , Aronne Antonio
Published: March 2023

Stimulated Raman scattering in transparent glass-ceramics (TGCs) based on bulk nucleating phase Ba2NaNb5O15 were investigated with the aim to explore the influence of micro- and nanoscale structural transformations on Raman gain. Nanostructured TGCs were synthesized, starting with 8BaO·15Na2O·27Nb2O5·50SiO2 (BaNaNS) glass, by proper nucleation and crystallization heat treatments. TGCs are composed of nanocrystals that are 10–15 nm in size, uniformly distributed in the residual glass matrix, with a crystallinity degree ranging from 30 up to 50% for samples subjected to different heat treatments. A significant Raman gain improvement for both BaNaNS glass and TGCs with respect to SiO2 glass is demonstrated, which can be clearly related to the nanostructuring process. These findings show that the nonlinear optical functionalities of TGC materials can be modulated by controlling the structural transformations at the nanoscale rather than microscale.
Controlled Synthesis and Enhanced Gas Sensing Performance of Zinc-Doped Indium Oxide Nanowires
Authors: Yu Che-Wen , Fu Hsuan-Wei , Yang Shu-Meng , Lin Yu-Shan , Lu Kuo-Chang
Published: March 2023

Indium oxide (In2O3) is a widely used n-type semiconductor for detection of pollutant gases; however, its gas selectivity and sensitivity have been suboptimal in previous studies. In this work, zinc-doped indium oxide nanowires with appropriate morphologies and high crystallinity were synthesized using chemical vapor deposition (CVD). An accurate method for electrical measurement was attained using a single nanowire microdevice, showing that electrical resistivity increased after doping with zinc. This is attributed to the lower valence of the dopant, which acts as an acceptor, leading to the decrease in electrical conductivity. X-ray photoelectron spectroscopy (XPS) analysis confirms the increased oxygen vacancies due to doping a suitable number of atoms, which altered oxygen adsorption on the nanowires and contributed to improved gas sensing performance. The sensing performance was evaluated using reducing gases, including carbon monoxide, acetone, and ethanol. Overall, the response of the doped nanowires was found to be higher than that of undoped nanowires at a low concentration (5 ppm) and low operating temperatures. At 300 °C, the gas sensing response of zinc-doped In2O3 nanowires was 13 times higher than that of undoped In2O3 nanowires. The study concludes that higher zinc doping concentration in In2O3 nanowires improves gas sensing properties by increasing oxygen vacancies after doping and enhancing gas molecule adsorption. With better response to reducing gases, zinc-doped In2O3 nanowires will be applicable in environmental detection and life science.
Progress in Research and Application of Metal–Organic Gels: A Review
Authors: Liu Gen , Li Siwen , Shi Chunyan , Huo Mingxin , Lin Yingzi
Published: March 2023

In recent years, metal–organic gels (MOGs) have attracted much attention due to their hierarchical porous structure, large specific surface area, and good surface modifiability. Compared with MOFs, the synthesis conditions of MOGs are gentler and more stable. At present, MOGs are widely used in the fields of catalysis, adsorption, energy storage, electrochromic devices, sensing, analysis, and detection. In this paper, literature metrology and knowledge graph visualization analysis are adopted to analyze and summarize the literature data in the field of MOGs. The visualization maps of the temporal distribution, spatial distribution, authors and institutions’ distribution, influence of highly cited literature and journals, keyword clustering, and research trends are helpful to clearly grasp the content and development trend of MOG materials research, point out the future research direction for scholars, and promote the practical application of MOGs. At the same time, the paper reviews the research and application progress of MOGs in recent years by combining keyword clustering, time lines, and emergence maps, and looks forward to their challenges, future development trend, and application prospects.
Chronic Kidney Disease—State of Either “Too Much” or “Too Little”
Authors: Brandenburg Vincent Mathias , Saritas Turgay
Published: March 2023

Chronic kidney disease (CKD) is a world-wide phenomenon with an increasing incidence and prevalence [...]
Circulating Metabolomic and Lipidomic Signatures Identify a Type 2 Diabetes Risk Profile in Low-Birth-Weight Men with Non-Alcoholic Fatty Liver Disease
Authors: Elingaard-Larsen Line O. , Villumsen Sofie O. , Justesen Louise , Thuesen Anne Cathrine B. , Kim Min , Ali Mina , Danielsen Else R. , Legido-Quigley Cristina , van Hall Gerrit , Hansen Torben , Ahluwalia Tarunveer S. , Vaag Allan A. , Brøns Charlotte
Published: March 2023

The extent to which increased liver fat content influences differences in circulating metabolites and/or lipids between low-birth-weight (LBW) individuals, at increased risk of type 2 diabetes (T2D), and normal-birth-weight (NBW) controls is unknown. The objective of the study was to perform untargeted serum metabolomics and lipidomics analyses in 26 healthy, non-obese early-middle-aged LBW men, including five men with screen-detected and previously unrecognized non-alcoholic fatty liver disease (NAFLD), compared with 22 age- and BMI-matched NBW men (controls). While four metabolites (out of 65) and fifteen lipids (out of 279) differentiated the 26 LBW men from the 22 NBW controls (p ≤ 0.05), subgroup analyses of the LBW men with and without NAFLD revealed more pronounced differences, with 11 metabolites and 56 lipids differentiating (p ≤ 0.05) the groups. The differences in the LBW men with NAFLD included increased levels of ornithine and tyrosine (PFDR ≤ 0.1), as well as of triglycerides and phosphatidylcholines with shorter carbon-chain lengths and fewer double bonds. Pathway and network analyses demonstrated downregulation of transfer RNA (tRNA) charging, altered urea cycling, insulin resistance, and an increased risk of T2D in the LBW men with NAFLD. Our findings highlight the importance of increased liver fat in the pathogenesis of T2D in LBW individuals.
Rare Sugar Metabolism and Impact on Insulin Sensitivity along the Gut–Liver–Muscle Axis In Vitro
Authors: van Laar Amar , Grootaert Charlotte , Rajkovic Andreja , Desmet Tom , Beerens Koen , Van Camp John
Published: March 2023

Rare sugars have recently attracted attention as potential sugar replacers. Understanding the biochemical and biological behavior of these sugars is of importance in (novel) food formulations and prevention of type 2 diabetes. In this study, we investigated whether rare sugars may positively affect intestinal and liver metabolism, as well as muscle insulin sensitivity, compared to conventional sugars. Rare disaccharide digestibility, hepatic metabolism of monosaccharides (respirometry) and the effects of sugars on skeletal muscle insulin sensitivity (impaired glucose uptake) were investigated in, respectively, Caco-2, HepG2 and L6 cells or a triple coculture model with these cells. Glucose and fructose, but not l-arabinose, acutely increased extracellular acidification rate (ECAR) responses in HepG2 cells and impaired glucose uptake in L6 cells following a 24 h exposure at 28 mM. Cellular bioenergetics and digestion experiments with Caco-2 cells indicate that especially trehalose (α1-1α), D-Glc-α1,2-D-Gal, D-Glc-α1,2-D-Rib and D-Glc-α1,3-L-Ara experience delayed digestion and reduced cellular impact compared to maltose (α1-4), without differences on insulin-stimulated glucose uptake in a short-term setup with a Caco-2/HepG2/L6 triple coculture. These results suggest a potential for l-arabinose and specific rare disaccharides to improve metabolic health; however, additional in vivo research with longer sugar exposures should confirm their beneficial impact on insulin sensitivity in humans.
Gestational Vitamin E Status and Gestational Diabetes Mellitus: A Retrospective Cohort Study
Authors: Shi Huifeng , Gong Xiaoli , Sheng Qing , Li Xiang , Wang Ying , Wu Tianchen , Zhao Yangyu , Wei Yuan
Published: March 2023

Objectives: To examine the association between vitamin E (VE) status and gestational diabetes mellitus (GDM). Methods: A retrospective cohort study was conducted by using data of 52,791 women at 137 hospitals across 22 provinces of China. A fasting plasma glucose (FPG) level of ≥5.1 mmol/L between the 24th and 40th weeks of gestation was used as the criteria for the diagnosis of GDM. Mean FPG level and GDM rate were calculated within each combination of the first-trimester VE concentration categories and gestational change categories. The associations of the first-trimester VE concentrations and gestational VE change with FPG and GDM were examined by employing generalized additive models (GAMs). Results: 7162 (13.57%) cases were diagnosed with GDM. The GDM rate was 22.44%, 11.50%, 13.41%, 12.87%, 13.17%, 13.44%, 12.64%, and 14.24% among women with the first-trimester VE concentrations of <7.2, 7.2–7.9, 8.0–9.3, 9.4–11.0, 11.1–13.2, 13.3–15.8, 15.9–17.7, and 17.8–35.9 mg/L, respectively. The GDM rate was 15.96%, 13.10%, 13.64%, and 12.87% among women with gestational VE change of <0, 0–0.19, 0.20–0.29, ≥0.30 mg/L per week, respectively. Multivariable adjusted GAM analyses found that the first-trimester VE concentration was associated with the FPG levels and GDM risk in an L-shaped pattern; the FPG levels and GDM risk decreased sharply to a threshold (around 7 mg/L), and then were keep flat. Gestational VE decreases when the first-trimester VE level was less than 11 mg/L were related to increased FPG levels and GDM risk. Conclusions: Both low first-trimester VE levels and subsequent gestational VE decrease were related with increased risk of GDM. The findings suggest the necessity of having VE-rich foods and appropriate VE supplementation to prevent GDM for pregnant women with low baseline VE levels.
Maternal Body Mass Index, Gestational Weight Gain, and Risk of Cancer in Offspring: A Systematic Review and Meta-Analysis
Authors: Miao Junxiang , Chen Yan , Liu Xiaoling , Ye Changxiang , Zhou Xuan , Yang Ziqi , Gong Ziqiang , Chen Lizhang , Wang Tingting
Published: March 2023

Background: Mounting evidence suggests that maternal obesity and gestational weight gain (GWG) may increase the risk of cancer in their offspring; however, results are inconsistent. The purpose of this research is to determine the association between maternal body mass index (BMI) and GWG and the risk of cancer in offspring through a systematic and comprehensive meta-analysis. Methods: A systematic literature search of several databases was conducted on 1 October 2022 to identify relevant studies. The quality of the included studies was evaluated using the Newcastle–Ottawa scale. The overall risk estimates were pooled using a random-effects meta-analysis. Results: Twenty-two studies with more than 8 million participants were included. An increased risk of total cancer was found in offspring whose mothers had a high GWG (odds ratio [OR]: 1.10; 95% CI: 1.01–1.19; p: 0.040) but not in offspring whose mothers had a low GWG (OR: 1.06; 95% CI: 0.96–1.17; p: 0.030), when compared with offspring whose mothers had a suitable GWG. In addition, no statistically significant association was found between maternal underweight (OR: 1.05; 95% CI: 0.97–1.13; p: 0.630), overweight/obesity (OR: 1.07; 95% CI: 0.99–1.16; p: 0.020), and risk of total cancer in offspring. Conclusions: Our study proposes evidence that maternal BMI and GWG may be associated with the risk of cancer in offspring, although statistical significance was found only for high GWG. Further well-designed research is required to clarify the potential relevance of maternal BMI and GWG on offspring cancer, especially for specific cancers.