bluuebunny/crossref_metadata_2025 huggingface dataset:
The circRAB3IP Mediated by eIF4A3 and LEF1 Contributes to Enzalutamide Resistance in Prostate Cancer by Targeting miR-133a-3p/miR-133b/SGK1 Pathway
Authors: Chen Dong , Wang Yaqin , Yang Feiya , Keranmu Adili , Zhao Qingxin , Wu Liyuan , Han Sujun , Xing Nianzeng
Published: November 2021

An increasing number of studies have shown that circRNAs are closely related to the carcinogenesis and development of prostate cancer (PCa). However, little is known about the effect of the biological functions of circRNAs on the enzalutamide resistance of PCa. Through bioinformatic analysis and experiments, we investigated the expression pattern of circRNAs in enzalutamide-resistant PCa cells. Quantitative real-time PCR was used to detect the expression of circRAB3IP, and plasmids that knock down or overexpress circRAB3IP were used to evaluate its effect on the enzalutamide sensitivity of PCa cells. Mechanistically, we explored the potential regulatory effects of eIF4A3 and LEF1 on the biogenesis of circRAB3IP. Our in vivo and in vitro data indicated that increased expression of circRAB3IP was found in enzalutamide-resistant PCa, and knockdown of circRAB3IP significantly enhanced enzalutamide sensitivity in PCa cells. However, upregulation of circRAB3IP resulted in the opposite effects. Further mechanistic research demonstrated that circRAB3IP could regulate the expression of serum and glucocorticoid-regulated kinase 1 (SGK1) by serving as a sponge that directly targets miR-133a-3p/miR-133b. Then, we showed that circRAB3IP partially exerted its biological functions via SGK1 signaling. Furthermore, we discovered that eIF4A3 and LEF1 might increase circRAB3IP expression in PCa.
EH Domain-Containing 2 Deficiency Restricts Adipose Tissue Expansion and Impairs Lipolysis in Primary Inguinal Adipocytes
Authors: Fryklund Claes , Morén Björn , Shah Shrenika , Grossi Mario , Degerman Eva , Matthaeus Claudia , Stenkula Karin G.
Published: November 2021

Lipid uptake can be facilitated via caveolae, specific plasma membrane invaginations abundantly expressed in adipocytes. The dynamin-related protein EH domain-containing 2 (EHD2) stabilizes caveolae at the cell surface. Here, we have examined the importance of EHD2 for lipid handling using primary adipocytes isolated from EHD2 knockout (Ehd2−/−) C57BL6/N mice. Following high-fat diet (HFD) feeding, we found a clear impairment of epididymal, but not inguinal, adipose tissue expansion in Ehd2−/− compared with Ehd2+/+ (WT) mice. Cell size distribution analysis revealed that Ehd2−/− mice had a lower proportion of small adipocytes, and an accumulation of medium-sized adipocytes in both epididymal and inguinal adipose tissue. Further, PPARγ activity, FABP4 and caveolin-1 expression were decreased in adipocytes isolated from Ehd2−/− mice. Inguinal adipocytes isolated from Ehd2−/− mice displayed reduced lipolysis in response to beta adrenergic receptor agonist, which was associated with reduced phosphorylation of perilipin-1 and hormone sensitive lipase (HSL). This impairment could not be rescued using a cAMP analog, indicating that impaired lipolysis in Ehd2−/− primary adipocytes likely occurs at the level of, or downstream of, protein kinase A (PKA). Altogether, these findings pinpoint the importance of EHD2 for maintained intracellular lipid metabolism, and emphasize differences in mechanisms regulating lipid handling in various adipose-tissue depots.
CRISPR/Cas9 and Transgene Verification of Gene Involvement in Unfolded Protein Response and Recombinant Protein Production in Barley Grain
Authors: Panting Michael , Holme Inger Baeksted , Björnsson Jón Már , Zhong Yingxin , Brinch-Pedersen Henrik
Published: November 2021

The use of plants as heterologous hosts to produce recombinant proteins has some intriguing advantages. There is, however, the potential of overloading the endoplasmic reticulum (ER) capacity when producing recombinant proteins in the seeds. This leads to an ER-stress condition and accumulating of unfolded proteins. The unfolded protein response (UPR) is activated to alleviate the ER-stress. With the aim to increase the yield of human epidermal growth factor (EGF) and mouse leukemia inhibitory factor (mLIF) in barley, we selected genes reported to have increased expression during ER-induced stress. The selected genes were calreticulin (CRT), protein disulfide isomerase (PDI), isopentenyl diphosphate isomerase (IPI), glutathione-s-transferase (GST), HSP70, HSP26, and HSP16.9. These were knocked out using CRISPR/Cas9 or overexpressed by conventional transgenesis. The generated homozygous barley lines were crossed with barley plants expressing EGF or mLIF and the offspring plants analyzed for EGF and mLIF protein accumulation in the mature grain. All manipulated genes had an impact on the expression of UPR genes when plantlets were subjected to tunicamycin (TN). The PDI knockout plant showed decreased protein body formation, with protein evenly distributed in the cells of the endosperm. The two genes, GST and IPI, were found to have a positive effect on recombinant protein production. mLIF expression was increased in a F2 homozygous GST knockout mutant background as compared to a F2 GST wild-type offspring. The overexpression of IPI in a F1 cross showed a significant increase in EGF expression. We demonstrate that manipulation of UPR related genes can have a positive effect on recombinant protein accumulation.
Composition of Flavonoids in the Petals of Freesia and Prediction of Four Novel Transcription Factors Involving in Freesia Flavonoid Pathway
Authors: Zhu Jiayi , Guo Xueying , Li Xin , Tang Dongqin
Published: November 2021

Freesia hybrida is rich in flower colors with beautiful flower shapes and pleasant aroma. Flavonoids are vital to the color formation of its flowers. In this study, five Freesia cultivars with different flower colors were used to study on the level of accumulation of their flavonoids and expression of flavonoid-related genes and further explore new novel transcription factor (TF). Ultra-high-performance liquid chromatography and VION ion mobility quadrupole time-of-flight mass spectrometer (UPLC-Q-TOF-MS) were used to determine the flavonoids. Combined with transcriptome sequencing technology, the molecular mechanism of the flavonoid metabolism difference in Freesia was revealed. A total of 10 anthoxanthin components and 12 anthocyanin components were detected using UPLC-Q-TOF-MS. All six common anthocyanin aglycones in high plants, including cyanidin, delphinidin, petunidin, peonidin, malvidin, and pelargonidin, were detected in Freesia at first time in this study. In orange, yellow, and white cultivars, anthoxanthins gradually decreased with the opening of the petals, while in red and purple cultivars, anthoxanthins first increased and then decreased. No anthocyanin was detected in yellow and white cultivars, while anthocyanins increased with the opening of the petals and reached their maximum at the flowering stage (S3) in other three cultivars. The correlation analysis revealed that the color of Freesia petals was closely related to the composition and content of anthoxanthins and anthocyanins. Petals of five cultivars at S3 were then selected for transcriptome sequencing by using the Illumina Hiseq 4000 platform, and a total of 100,539 unigenes were obtained. There were totally 5,162 differentially expressed genes (DEGs) when the four colored cultivars were compared with the white cultivar at S3. Comparing all DEGs with gene ontology (GO), KEGG, and Pfam databases, it was found that the genes involved in the flavonoid biosynthesis pathway were significantly different. In addition, AP2, WRKY, and bHLH TF families ranked the top three among all differently expressed TFs in all DEGs. Quantitative real-time PCR (qRT-PCR) technology was used to analyze the expression patterns of the structural genes of flavonoid biosynthesis pathway in Freesia. The results showed that metabolic process was affected significantly by structural genes in this pathway, such as CHS1, CHI2, DFR1, ANS1, 3GT1, and FLS1. Cluster analysis was performed by using all annotated WRKY and AP2 TFs and the above structural genes based on their relatively expression. Four novel candidate TFs of WRKY and AP2 family were screened. Their spatiotemporal expression patterns revealed that these four novel TFs may participate in the regulation of the flavonoid biosynthesis, thus controlling its color formation in Freesia petals.
Bibliometric Analysis of Post Covid-19 Management Strategies and Policies in Hospitality and Tourism
Authors: Khan Kanwal Iqbal , Nasir Adeel , Saleem Saima
Published: November 2021

The strategic perspective of management policies gained utmost importance during the post-Covid era. The researchers are trying to introduce strategies that can help organizations cope with post-crisis destruction. Yet, the research on the topic is fragmented, mainly related to the hospitality and tourism industry. This manuscript aims to present scholarly research findings dealing with the post-Covid-19 management strategies in the hospitality and tourism industry from January 1, 2020, to July 28, 2021. These strategies can play an essential role in the survival and growth of the sectors. The study identified and acknowledged the core contributing authors, journals, countries, affiliation, corresponding authors through bibliometric, citation, and keyword analysis. It also conducted the co-occurrence analysis and reported three significant research streams and bibliometric coupling to identify four research themes for management strategies of the tourism and hospitality industry in the post-Covid era. With the help of an influential and conceptual framework, the study highlights the future challenges managers could face and suggests the possible area for reviewing and revising the existing policies by proposing future directions. Consequently, this study contributes to the current literature on post-Covid-19 management strategies and policies by developing the critical analysis of the extant literature and highlighting the understudy areas that future studies must explore to expand the scope of the research.
Predicting Work-Life Conflict: Types and Levels of Enacted and Preferred Work-Nonwork Boundary (In)Congruence and Perceived Boundary Control
Authors: Mellner Christin , Peters Pascale , Dragt Maria Johanna , Toivanen Susanna
Published: November 2021

In 2020, everyday life changed dramatically for employees worldwide as a result of the outbreak of the Covid-19 pandemic, where an estimated 558 million employees started working from home. The pandemic, therefore, marks a fundamental shift of individuals’ work-nonwork boundaries, which can impact work-life conflict. In particular, the interplay between individuals’ enacted boundaries (degree to which they separate/segment or blend/integrate work-nonwork), preferred boundaries (degree of preferred segmentation or integration of work-nonwork), and perceived control over work-nonwork boundaries, may relate to work-life conflict. This study, the first to the best of our knowledge, examines whether different types and levels of work-nonwork boundary (in)congruence matter for work-life conflict, and whether perceived boundary control moderates these relationships. Boundary (in)congruence represents the degree of (mis)fit between enacted and preferred segmentation or integration. Several types of (in)congruence are distinguished: “segmentation congruence” (enacting and preferring segmentation); “integration congruence” (enacting and preferring integration); “intrusion” (enacting integration but preferring segmentation) and “distance” (enacting segmentation but preferring integration). Data from 1,229 managers working in public and private organizations in Sweden was analyzed using polynomial regression analysis with response surface modeling and moderation analysis in SPSS Process. Findings showed that “integration congruence” was related with higher work-life conflict than “segmentation congruence.” Moreover, a U-shaped relationship between incongruence and work-life conflict was found: the more incongruence, the more work-life conflict. Specifically, “intrusion” was related to higher work-life conflict than “distance.” Finally, boundary control mitigated the effect of incongruence (especially “intrusion”) on work-life conflict. From our findings, we may conclude that work-life conflict is impacted differently depending on the type and level of boundary (in)congruence. Particularly enacted and/or preferred integration may be problematic when it comes to work-life conflict, rather than just (in)congruence per se. Moreover, boundary control can be viewed as a key factor in combating work-life conflict, especially among individuals who enact integration, but prefer segmentation. Taken together, our study contributes new and substantial knowledge by showing the importance for research and HRM-policies that take into account different types and levels of boundary (in)congruence, as these are associated with different levels of work-life conflict, which, in turn, are moderated by boundary control.
Artificial Intelligence: An Interprofessional Perspective on Implications for Geriatric Mental Health Research and Care
Authors: Renn Brenna N. , Schurr Matthew , Zaslavsky Oleg , Pratap Abhishek
Published: November 2021

Artificial intelligence (AI) in healthcare aims to learn patterns in large multimodal datasets within and across individuals. These patterns may either improve understanding of current clinical status or predict a future outcome. AI holds the potential to revolutionize geriatric mental health care and research by supporting diagnosis, treatment, and clinical decision-making. However, much of this momentum is driven by data and computer scientists and engineers and runs the risk of being disconnected from pragmatic issues in clinical practice. This interprofessional perspective bridges the experiences of clinical scientists and data science. We provide a brief overview of AI with the main focus on possible applications and challenges of using AI-based approaches for research and clinical care in geriatric mental health. We suggest future AI applications in geriatric mental health consider pragmatic considerations of clinical practice, methodological differences between data and clinical science, and address issues of ethics, privacy, and trust.
Estimating the Cost of the Design, Production, and Dissemination of Social Media Videos for Social and Behavioral Change: Evidence From Merci Mon Héros in Niger and Côte d'Ivoire
Authors: Tchuenche Michel , Bellows Nicole , Portillo Erin , Labati Zamilatou H. , Adou Denise B. , Hammond Jacqueline , Silva Martha , Bollinger Lori
Published: November 2021

Merci Mon Héros (MMH) is a youth-led multi-media campaign in Francophone West Africa seeking to improve reproductive health and family planning outcomes using radio, television, social media, and community events. One component to this project is the development of a series of youth-driven videos created to encourage both youth and adults to break taboos by talking to each other about reproductive health and family planning. A costing study was conducted to capture costs associated with the design, production, and dissemination of 11 MMH videos (in French) on social media in Côte d'Ivoire and Niger. The total costs to design, produce and disseminate 11 of the campaign videos for MMH in both Côte d'Ivoire and Niger were $44,981. Unit costs were calculated using three different denominators, resulting in average unit costs of $0.16 per reach, $1.29 per engagement, and $4.27 per video view. These findings can be useful for future studies of SBC interventions using social media for framing the analysis and selecting the appropriate metrics for the denominator, as well as for budgeting and planning SBC programs using social media.
Quantifying the Health Benefits of Urban Climate Mitigation Actions: Current State of the Epidemiological Evidence and Application in Health Impact Assessments
Authors: Castillo Maria D. , Anenberg Susan C. , Chafe Zoe A. , Huxley Rachel , Johnson Lauren S. , Kheirbek Iyad , Malik Maha , Marshall Julian D. , Naidoo Seneca , Nelson Margaret L. , Pendleton Nicholas V. , Sun Yuantong , van den Broek d'Obrenan Honorine , Kinney Patrick L.
Published: November 2021

While ambitious carbon reduction policies are needed to avoid dangerous levels of climate change, the costs of these policies can be balanced by wide ranging health benefits for local communities. Cities, responsible for ~70% of the world's greenhouse gas (GHG) emissions and home to a growing majority of the world's population, offer enormous opportunities for both climate action and health improvement. We aim to review the current state of knowledge on key pathways leading from carbon mitigation to human health benefits, and to evaluate our current ability to quantify health benefits for cities around the world. For example, because GHGs and air pollutants are both released during fuel combustion, reducing fuel burning can reduce both GHGs and air pollutants, leading to direct health benefits. Air quality improvements may be particularly important for city-scale climate action planning because the benefits occur locally and relatively immediately, compared with the global and long-term (typically, decades to centuries) benefits for the climate system. In addition to improved air quality, actions that promote active transport in cities via improved cycling and pedestrian infrastructure can reap large cardiovascular health benefits via increased physical activity. Exposure to green space has been associated with beneficial health outcomes in a growing number of epidemiological studies and meta-analyses conducted around the world. Finally, noise is an underappreciated environmental risk factor in cities which can be addressed through actions to reduce motor vehicle traffic and other noise sources. All of these environmental health pathways are supported by well-conducted epidemiological studies in multiple locales, providing quantitative exposure–response data that can be used as inputs to health impact assessments (HIAs). However, most epidemiologic evidence derives from studies in high-income countries. It is unclear to what extent such evidence is directly transferable for policies in low- and middle-income countries (LMICs). This gap calls for a future focus on building the evidence based in LMIC cities. Finally, the literature suggests that policies are likely to be most effective when they are developed by multidisciplinary teams that include policy makers, researchers, and representatives from affected communities.
Population Pharmacokinetics of Intravenous Amoxicillin Combined With Clavulanic Acid in Healthy and Critically Ill Dogs
Authors: Vegas Cómitre Maria D. , Cortellini Stefano , Cherlet Marc , Devreese Mathias , Roques Beatrice B. , Bousquet-Melou Alain , Toutain Pierre-Louis , Pelligand Ludovic
Published: November 2021

Background: Data regarding antimicrobial pharmacokinetics (PK) in critically ill dogs are lacking and likely differ from those of healthy dogs. The aim of this work is to describe a population PK model for intravenous (IV) amoxicillin–clavulanic acid (AMC) in both healthy and sick dogs and to simulate a range of clinical dosing scenarios to compute PK/PD cutoffs for both populations.

Methods: This study used a prospective clinical trial in normal and critically ill dogs. Twelve client-owned dogs hospitalized in the intensive care unit (ICU) received IV AMC 20 mg/kg every 8 h (0.5-h infusion) during at least 48 h. Eight blood samples were collected at predetermined times, including four trough samples before the next administration. Clinical covariates and outcome were recorded, including survival to discharge and bacteriologic clinical failure. Satellite PK data were obtained de novo from a group of 12 healthy research dogs that were dosed with a single AMC 20 mg/kg IV. Non-linear mixed-effects model was used to estimate the PK parameters (and the effect of health upon them) together with variability within and between subjects. Monte Carlo simulations were performed with seven dosage regimens (standard and increased doses). The correlation between model-derived drug exposure and clinical covariates was tested with Spearman's non-parametric correlation analysis. Outcome was recorded including survival to discharge and bacteriologic clinical failure.

Results: A total of 218 amoxicillin concentrations in plasma were available for healthy and sick dogs. A tricompartmental model best described the data. Amoxicillin clearance was reduced by 56% in sick dogs (0.147 L/kg/h) compared with healthy dogs (0.336 L/kg/h); intercompartmental clearance was also decreased (p <0.01). None of the clinical data covariates were significantly correlated with individual exposure. Monte Carlo simulations showed that higher PK/PD cutoff values of 8 mg/L could be reached in sick dogs by extending the infusion to 3 h or doubling the dose.

Conclusions: The PK of AMC is profoundly different in critically ill dogs compared with normal dogs, with much higher interindividual variability and a lower systemic clearance. Our study allows to generate hypotheses with regard to higher AMC exposure in clinical dogs and provides supporting data to revise current AMC clinical breakpoint for IV administration.
New Tools for Mechanical Thinning of Apricot Fruitlets
Authors: Assirelli Alberto , Caracciolo Giuseppina , Roccuzzo Giancarlo , Stagno Fiorella
Published: November 2021

In this study, the thinner machine with yellow rod equipment was tested in relation to tree branch length and orientation in April 2019, in a narrow-canopied apricot orchard of Emilia Romagna Region, Italy. The trees were mechanically thinned with manual finishing, and comparative tests were carried out simultaneously with the ordinary hand thinning (control). Three groups of two plants were identified as replication for a total of six plants per row. Three rows were checked, considering field uniformity average. The branches were grouped into four classes according to their length: <30 cm, 30–60 cm, 60–90 cm and >90 cm. Branch inclination on the plant, radial or longitudinal with respect to the row, was evaluated. Fruit number before the thinning, after the first and the second machine intervention, after three days of the mechanical thinning and after the hand finishing was recorded. This experience showed satisfactory results in terms of thinning efficiency and reduced damage to both fruits and branches, as a function of the class length and insertion point in the main branch of the plant. Thinning efficiency was always kept above 37% of the left load after hand finishing, and on average between the treatments close to 44%. Fruit damages always remained below the economic thresholds to marketable production or to the plant.
Study on Plant Growth and Nutrient Uptake under Different Aeration Intensity in Hydroponics with the Application of Particle Image Velocimetry
Authors: Baiyin Bateer , Tagawa Kotaro , Yamada Mina , Wang Xinyan , Yamada Satoshi , Yamamoto Sadahiro , Ibaraki Yasuomi
Published: November 2021

Aeration is considered beneficial for hydroponics. However, little information is available on the effects of aeration, and even less on solutions that use bubble flow and their agronomic effects. In this study, the effects of aeration intensity on plants were studied through cultivation experiments and flow field visualization. It was found that the growth of plants did not increase linearly with an increase in aeration intensity. From the results of this study, when the aeration intensity was within the low range (0.07–0.15 L·L−1 NS·min−1), increasing the aeration intensity increased the plant growth. However, after the aeration intensity reached a certain extent (0.15–1.18 L·L−1 NS·min−1), some indicators did not change significantly. When the aeration intensity continued to increase (1.18–2.35 L·L−1 NS·min−1), growth began to decrease. These results show that for increasing dissolved oxygen and promoting plant growth, the rule is not “the higher the aeration intensity, the better”. There is a reasonable range of aeration intensity within which crops grow normally and rapidly. In addition, increasing the aeration intensity means increasing energy utilization and operating costs. In actual hydroponics production, it is very important to find a reasonable aeration intensity range.
A Review of Methods and Techniques for Detecting Frost on Plant Surfaces
Authors: Song Huan , Hu Yongguang , Lu Yongzong , Wang Jizhang , Pan Qingmin , Li Pingping
Published: November 2021

Severe frost usually has adverse impacts on agricultural production, resulting in crop freeze injury, poor crop yield, and crop quality reduction. Timely and accurate detection of frost plays an important role in cold damage warnings, prevention, and control. Current frost detection methods mostly use physical properties such as light, electricity, and heat, or the judge and quantify using environmental factors such as temperature and wind speed. However, it is difficult to detect and accurately identify the frosting phenomenon in real time during field trials because of the complex environment, different plant types, and interference by many factors during observation. To provide an overview of the analytical tools for scientists, researchers, and product developers, a review and comparative analysis of the available literature on frost mechanisms, correlations, and characteristics are presented in this study. First, the mechanisms of the frost formation process, frost level, and the significance of detection, are introduced. Then, the methods and techniques used to measure frost on plant surfaces are synthetically classified and further compared. Moreover, the key points and difficulties are summarized and discussed. Finally, some constructive methods of frost detection are proposed to improve the frost detection process.
An Analysis of Demographic and Triage Assessment Findings in Bushfire-Affected Koalas (Phascolarctos cinereus) on Kangaroo Island, South Australia, 2019–2020
Authors: Dunstan Evie , Funnell Oliver , McLelland Jenny , Stoeckeler Felicity , Nishimoto Elisa , Mitchell Dana , Mitchell Sam , McLelland David J. , Kalvas Jerome , Johnson Lynley , Moore Claire , Eyre Lauren J. M. , McLune Amanda , Hough Ian , Valenza Ludovica , Boardman Wayne S. J. , Smith Ian , Speight Natasha
Published: November 2021

In the 2019–2020 Australian bushfires, Kangaroo Island, South Australia, experienced catastrophic bushfires that burnt approximately half the island, with an estimated 80% of the koala population lost. During and after the event, rescued koalas were triaged at a designated facility and a range of initial data were recorded including rescue location and date, sex, estimation of age, body condition and hydration, and assessment of burn severity (n = 304 records available). Koalas were presented to the triage facility over a span of 10 weeks, with 50.2% during the first 14 days of the bushfire response, the majority of which were rescued from regions of lower fire severity. Burns were observed in 67.4% of koalas, with the majority (60.9%) classified as superficial burns, primarily affecting the limbs and face. Poor body condition was recorded in 74.6% of burnt koalas and dehydration in 77.1%. Negative final outcomes (death or euthanasia, at triage or at a later date) occurred in 45.6% of koalas and were significantly associated with higher mean burn score, maximum burn severity, number of body regions burnt, poor body condition score, and dehydration severity. The findings of this retrospective study may assist clinicians in the field with decision making when triaging koalas in future fire rescue efforts.
A Review of Bacterial Co-Infections in Farmed Catfish: Components, Diagnostics, and Treatment Directions
Authors: Wise Allison L. , LaFrentz Benjamin R. , Kelly Anita M. , Khoo Lester H. , Xu Tingbi , Liles Mark R. , Bruce Timothy J.
Published: November 2021

Catfish production is a major aquaculture industry in the United States and is the largest sector of food fish production. As producers aim to optimize production yields, diseases caused by bacterial pathogens are responsible for high pond mortality rates and economic losses. The major bacterial pathogens responsible are Edwardsiella ictaluri, Aeromonas spp., and Flavobacterium columnare. Given the outdoor pond culture environments and ubiquitous nature of these aquatic pathogens, there have been many reports of co-infective bacterial infections within this aquaculture sector. Co-infections may be responsible for altering disease infection mechanics, increasing mortality rates, and creating difficulties for disease management plans. Furthermore, proper diagnoses of primary and secondary pathogens are essential in ensuring the correct treatment approaches for antimicrobials and chemical applications. A thorough understanding of the interactions and infectivity dynamics for these warm water bacterial pathogens will allow for the adoption of new prevention and control methods, particularly in vaccine development. This review aims to provide an overview of co-infective pathogens in catfish culture and present diagnostic case data from Mississippi and Alabama to define prevalence for these multiple-species infections better.
Reproductive Potential Accelerates Preimaginal Development of Rebel Workers in Apis mellifera
Authors: Strachecka Aneta , Olszewski Krzysztof , Kuszewska Karolina , Paleolog Jerzy , Woyciechowski Michał
Published: November 2021

Rebel workers develop from eggs laid by the previous queen, before it went swarming and left the colony orphaned, until the emergence of a new queen. In contrast to normal workers developing in the queen’s presence, rebels are set to reproduce and avoid rearing of successive bee generations. They have more ovarioles in their ovaries, as well as more developed mandibular glands and underdeveloped hypopharyngeal glands, just like the queen. We posited that rebels are not only similar to queens in some anatomical features, but also develop in a shorter time in comparison to normal workers. Therefore, the aim of this study was to compare preimaginal development duration in rebel and normal workers. The results show that rebels, i.e., workers with a higher reproductive potential, had a significantly shorter preimaginal development period (mean ± SD, 19.24 ± 0.07 days) than normal workers (22.29 ± 0.32 days). Our result confirmed that workers who develop in a queen-less colony undergo a shorter preimaginal development than those in a queen-right colony.
Evaluation of Welfare in Commercial Turkey Flocks of Both Sexes Using the Transect Walk Method
Authors: Hrženjak Nina Mlakar , Hristov Hristo , Dovč Alenka , Martinjak Jana Bergoč , Šemrov Manja Zupan , Žlabravec Zoran , Račnik Jožko , Krapež Uroš , Slavec Brigita , Rojs Olga Zorman
Published: November 2021

The study was conducted between March and September 2019 in six meat-type turkey flocks with similar management standard procedures using the transect walk method. The concept of the method is based on visual observation of the birds while slowly walking across the entire farm in predetermined transects. Each flock was evaluated at three different times during the fattening cycle: at 3 to 4, 12 to 13, and 19 to 20 weeks of age, and total number of males and females that were immobile or lame, had visible head, vent, or back wounds, were small, featherless, dirty, or sick, had pendulous crop, or showed aggression toward birds or humans were recorded. At each visit, NH3 and CO2 were measured within the facilities. In the first assessment, the most frequently observed welfare indicators were small size (0.87%) and immobility (0.08%). Males showed a significantly higher prevalence of small size (p < 0.01), sickness (p < 0.05), and dirtiness (p < 0.1) compared to females. In the second assessment, the most common findings in both sexes were dirtiness (1.65%) and poor feather condition (1.06%), followed by immobility (0.28%). Males were significantly dirtier (p < 0.001), had more immobile birds (p < 0.01) and birds with vent wounds (p < 0.1), but had fewer sick birds (p < 0.05). In the last assessment, an increase in immobile, lame, sick, and dead birds was recorded, indicating an increase in health problems. Higher CO2 (3000 and 4433 ppm) and NH3 (40 and 27.6 ppm) values were noted only at the first assessment in two facilities. Further analyses showed that slightly elevated NH3 and CO2 levels did not influence the occurrence of welfare indicators. This study is the first description of the welfare of commercial turkey flocks in Slovenia.
The Role of Birds of the Family Corvidae in Transmitting Sarcocystis Protozoan Parasites
Authors: Juozaitytė-Ngugu Evelina , Švažas Saulius , Šneideris Donatas , Rudaitytė-Lukošienė Eglė , Butkauskas Dalius , Prakas Petras
Published: November 2021

Members of the family Corvidae are ecologically flexible omnivorous birds, particularly adaptive to urban habitats, and living in proximity to humans; these birds may serve as definitive hosts (DH) for Sarcocystis spp., but research about this is lacking. In the present study, intestinal samples from 91 corvids collected in Lithuania were molecularly tested by species-specific PCR targeting the ITS1 and cox1 genes and subsequently sequenced for the presence of Sarcocystis spp. Under a light microscope, oocysts of Sarcocystis spp. were observed in 43 samples (47.3%), while molecular methods, detected Sarcocystis spp. in 77 birds (84.6%). Eleven Sarcocystis spp. (S. columbae, S. cornixi, potentially pathogenic S. halieti, S. kutkienae, S. lari, S. turdusi, S. wobeseri, S. arctica, S. lutrae, S. ovalis, and S. oviformis) were identified in the intestinal samples from six corvid species from Lithuania. Infections with multiple Sarcocystis spp. were detected in 79.2% of the infected corvid birds. Three of the identified Sarcocystis spp. use corvids as intermediate hosts (IH); therefore, corvids may serve as IH and DH of the same Sarcocystis species. Based on molecular results and on corvid diet, omnivorous corvids may play an important role in transmitting Sarcocystis spp.
Aerobic Isolates from Gestational and Non-Gestational Lactating Bitches (Canis lupus familiaris)
Authors: Vasiu Iosif , Meroni Gabriele , Dąbrowski Roman , Martino Piera Anna , Tvarijonaviciute Asta , Bochniarz Mariola , Pop Raul Alexandru , Brudaşcă Florinel Gheorghe , Fiţ Nicodim Iosif
Published: November 2021

Mastitis is a complex and well-defined mammary gland pathology, and an emergency in bitches. In dogs, its prevalence is about 1% of all reported diseases and about 5.3% of all reproductive pathologies. Lactating bitches are naturally prone to developing mastitis since puppies can easily overstimulate the epidermal layer of nipples during feeding, facilitating bacterial colonization of the glands. This study aimed to describe the aerobic bacterial flora isolated from milk samples derived from a cohort of patients (n = 87) diagnosed with clinical mastitis (n = 29), subclinical mastitis (n = 17) and healthy mammary glands (n = 46). All of the patients underwent a gynecology consultation to diagnose mammary gland afflictions; physical examination results were coupled with traditional hematological findings. The milk samples were plated on specific microbiological media for bacterial isolation. Among the 162 milk samples analyzed, 93.2% (151/162) had a positive microbiological result, while 6.8% (11/162) were sterile. The bacteriological profile of the milk samples showed 47 different species. The most common bacterial families detected in healthy bitches and bitches with subclinical and clinical mastitis were the Staphylococcaceae, Enterobacteriaceae and Enterococcaceae families. The results indicated that half of the isolated bacteria are novel findings in dogs and that some of them are normal components of human milk.
Anthropomorphism and Its Adverse Effects on the Distress and Welfare of Companion Animals
Authors: Mota-Rojas Daniel , Mariti Chiara , Zdeinert Andrea , Riggio Giacomo , Mora-Medina Patricia , del Mar Reyes Alondra , Gazzano Angelo , Domínguez-Oliva Adriana , Lezama-García Karina , José-Pérez Nancy , Hernández-Ávalos Ismael
Published: November 2021

Anthropomorphic practices are increasing worldwide. Anthropomorphism is defined as the tendency to attribute human forms, behaviors, and emotions to non-human animals or objects. Anthropomorphism is particularly relevant for companion animals. Some anthropomorphic practices can be beneficial to them, whilst others can be very detrimental. Some anthropomorphic behaviors compromise the welfare and physiology of animals by interfering with thermoregulation, while others can produce dehydration due to the loss of body water, a condition that brings undesirable consequences such as high compensatory blood pressure and heat shock, even death, depending on the intensity and frequency of an animal’s exposure to these stressors. Malnutrition is a factor observed due to consumption of junk food or an imbalance in caloric proportions. This can cause obesity in pets that may have repercussions on their locomotor apparatus. Intense human–animal interaction can also lead to the establishment of attachment that impacts the mental state and behavior of animals, making them prone to develop aggression, fear, or anxiety separation syndrome. Another aspect is applying cosmetics to pets, though scientific studies have not yet determined whether cosmetic products such as coat dyes, nail polish, and lotions are beneficial or harmful for the animals, or to what extent. The cohabitation of animals in people’s homes can also constitute a public health risk due to infectious and zoonotic diseases. In this context, this paper aims to analyze the adverse effects of anthropomorphism on the welfare of companion animals from several angles—physiological, sanitary, and behavioral—based on a discussion of current scientific findings.
Effect of Acacia mearnsii Tannin Extract Supplementation on Reproductive Performance and Oxidative Status of South African Mutton Merino Rams
Authors: Ahmed Osman , Lehloenya Khoboso , Mphaphathi Masindi , Hassen Abubeker
Published: November 2021

We investigated the supplementation effects of Acacia mearnsii tannin extract (TE) and encapsulated tannin extract (ETE) on reproductive performance and oxidative status of South African Mutton Merino rams. We also observed the season effect on the testicular measurements. Thirty rams were divided into five groups: 0.0 g TE (control), 1.5 g TE, 3 g TE, 1.5 g ETE, and 3 g ETE supplemented daily for 16 weeks transiting from autumn to winter. Bodyweight and testicular measurements were recorded biweekly. Semen and blood samples were collected weekly during the last five weeks of supplementation. Results showed that the increase in the ETE from 1.5 to 3 g increased the testicular length and sperm concentration, as well as decreased the percentages of low and non-progressive spermatozoa (p<0.05). Simultaneously, the increase in the TE from 1.5 to 3 g decreased semen volume and elevated the percentage of abnormal sperm (p<0.05). The results suggest that the encapsulation of TE affords the maximum benefit of the TE on the sperm quality. Treatments did not affect the hormonal and oxidative status. Testicular measurements were significantly higher in autumn compared to winter. The plasma level of cortisol significantly correlates negatively with sperm motility, viability, and acrosome integrity.
Dose Optimization of Vancomycin for Critically Ill Patients Undergoing CVVH: A Prospective Population PK/PD Analysis
Authors: Wang Chuhui , Zhang Chao , Li Xiaoxiao , Zhao Sixuan , He Na , Zhai Suodi , Ge Qinggang
Published: November 2021

The optimal dose of vancomycin in critically ill patients receiving continuous venovenous hemofiltration (CVVH) remains unclear. The objective of this study was to identify factors that significantly affect pharmacokinetic profiles and to further investigate the optimal dosage regimens for critically ill patients undergoing CVVH based on population pharmacokinetics and pharmacodynamic analysis. A prospective population pharmacokinetic analysis was performed at the surgical intensive care unit in a level A tertiary hospital. We included 11 critically ill patients undergoing CVVH and receiving intravenous vancomycin. Serial blood samples were collected from each patient, with a total of 131 vancomycin concentrations analyzed. Nonlinear mixed effects models were developed using NONMEM software. Monte Carlo Simulation was used to optimize vancomycin dosage regimens. A two-compartment model with first-order elimination was sufficient to characterize vancomycin pharmacokinetics for CVVH patients. The population typical vancomycin clearance (CL) was 1.15 L/h and the central volume of distribution was 16.9 L. CL was significantly correlated with ultrafiltration rate (UFR) and albumin level. For patients with normal albumin and UFR between 20 and 35 mL/kg/h, the recommended dosage regimen was 10 mg/kg qd. When UFR was between 35 and 40 mL/kg/h, the recommended dosage regimen was 5 mg/kg q8h. For patients with hypoalbuminemia and UFR between 20 and 25 mL/kg/h, the recommended dosage regimen was 5 mg/kg q8h. When UFR was between 25 and 40 mL/kg/h, the recommended dosage regimen was 10 mg/kg q12h. We recommend clinicians choosing the optimal initial vancomycin dosage regimens for critically ill patients undergoing CVVH based on these two covariates.
Sub-Inhibitory Antibiotic Exposure and Virulence in Pseudomonas aeruginosa
Authors: Nolan Charlotte , Behrends Volker
Published: November 2021

Pseudomonas aeruginosa is a prime opportunistic pathogen, one of the most important causes of hospital-acquired infections and the major cause of morbidity and mortality in cystic fibrosis lung infections. One reason for the bacterium’s pathogenic success is the large array of virulence factors that it can employ. Another is its high degree of intrinsic and acquired resistance to antibiotics. In this review, we first summarise the current knowledge about the regulation of virulence factor expression and production. We then look at the impact of sub-MIC antibiotic exposure and find that the virulence–antibiotic interaction for P. aeruginosa is antibiotic-specific, multifaceted, and complex. Most studies undertaken to date have been in vitro assays in batch culture systems, involving short-term (<24 h) antibiotic exposure. Therefore, we discuss the importance of long-term, in vivo-mimicking models for future work, particularly highlighting the need to account for bacterial physiology, which by extension governs both virulence factor expression and antibiotic tolerance/resistance.
Beneficial Effects of Anticoagulants on the Clinical Outcomes of COVID-19 Patients
Authors: Jamil Zubia , Khan Azmat Ali , Khalid Samreen , Asghar Muhammad , Muhammad Khalid , Waheed Yasir
Published: November 2021

(1) Background: Severe coronavirus disease can be complicated by a hypercoagulable state in conjunction with sepsis, increasing the risk of venous thromboembolism. This study aimed to observe the effect of anticoagulants on 30-day high-dependency unit (HDU) outcomes of moderate to severe coronavirus disease 2019 (COVID-19) patients of a tertiary care hospital at Rawalpindi, Pakistan. (2) Methods: A retrospective propensity-based case–control study was carried out to examine COVID-19 patients admitted to the HDU. Patient groups who did and did not receive anticoagulants were labeled as “anticoagulant” and “non-anticoagulant”, respectively. Case–control matching (1:1) was performed via propensity scores (calculated by a regression model). Kaplan–Meier and logrank analyses were used to study survival probability. Single predictors of outcomes were determined by Cox regression analysis. (3) Results: The anticoagulant group had elevated D-dimers, advanced age, more comorbidities and a higher frequency of severe disease compared to the non-anticoagulant group (p < 0.05). Therefore, 47 cases and 47 matched controls were selected based on their propensity scores. The primary endpoint was outcome (survived vs. died). The 30-day in-HDU mortality was 25.5% for cases and 61.7% for controls (p = 0.0004). The median time from admission to death was 16 days for the case group and 7 days for the control group (p < 0.0001). The 30-day mortality was 19.1% for the enoxaparin group and 16.4% for the heparin group (p > 0.05). Enoxaparin (therapeutic and prophylactic doses) and heparin (prophylactic dose) were found to be independent factors affecting the outcomes of these patients (p < 0.001). (4) Conclusions: Anticoagulants play a beneficial role in reducing mortality among COVID-19 patients. Both anticoagulant formulations, enoxaparin (therapeutic and prophylactic doses) and heparin (prophylactic dose), were associated with improving survival among these patients.
A Specific Mixture of Propolis and Carnosic Acid Triggers a Strong Fungicidal Action against Cryptococcus neoformans
Authors: Argüelles Alejandra , Sánchez-Fresneda Ruth , Martínez-Mármol Elisa , Lozano José Antonio , Solano Francisco , Argüelles Juan Carlos
Published: November 2021

Current antifungal chemotherapy against the prevalent basidiomycete Cryptococcus neoformans displays some drawbacks. This pathogenic fungus is refractory to echinocandins, whereas conventional treatment with amphotericin B plus 5-fluorocytosine has a limited efficacy. In this study, we explored the potential cryptococcal activity of some natural agents. After conducting a screening test with a set of propolis from different geographical areas, we selected an extract from China, which displayed a certain cytotoxic activity against C. neoformans, due to this extract being cheap and easily available in large amounts. The combination of this kind of propolis with carnosic acid in a 1:4 ratio induced a stronger fungicidal effect, which occurred following a synergistic pattern, without visible alterations in external cell morphology. Furthermore, several carnosic acid–propolis formulations applied onto preformed biofilms decreased the metabolic activity of the sessile cells forming biofilms. These data support the potential application of mixtures containing these two natural extracts in the design of new antifungal strategies in order to combat opportunistic infections caused by prevalent pathogenic fungi.
Effects of Metronidazole as an Adjunct to Non-Surgical Periodontal Therapy on Insulin Resistance in Type 2 Diabetics
Authors: Qureshi Ambrina , Haque Zeba , Qureshi Hina , Farooqui Waqas Ahmed
Published: November 2021

Treating periodontitis with metronidazole (MET) as an adjunct to scaling root planing (SRP) is suggested to have inconsistent effects on insulin resistance (IR) in type 2 diabetes mellitus (T2DM). This paper will present the effects of MET, in addition to SRP, on the homeostatis model assessment of IR (HOMA-IR). A three-arm clinical trial was conducted and analyses were performed on T2DM participants with periodontitis (n = 74) who completed follow-up visits at 3 and 6 months after the intervention. The observed between-group and within-group mean changes in IR were found using ANOVA with repeated measures, followed by a post-hoc analysis, and a p-value of ≤0.05 was considered significant. Between-group analyses showed no difference in the HOMA-IR at 3 months, but at 6 months the difference was significant (p = 0.046). Within-group analyses showed that the HOMA-IR was significantly reduced in both test groups (p ≤ 0.05) over the period of time. Adjunct use of MET may result in a sudden short-term lowering of the HOMA-IR level within 3 months that may not be retained over 6 months when compared to the sustained lowering of the HOMA-IR levels in T2DM when intervened with SRP without MET.