bluuebunny/crossref_metadata_2025 huggingface dataset:
Micro-Slab Coil Design for Hyperpolarized Metabolic Flux Analysis in Multiple Samples
Authors: Lee Geonhui , Ruan Thomas , Wong Claudia , Deh Kofi , Abolarin Alli , Correa Alexander , Keshari Kayvan R. , Jeong Sangmoo
Published: December 2022

Abnormal metabolism is a hallmark of cancer cells. Accumulating evidence suggests that metabolic changes are likely to occur before other cellular responses in cancer cells upon drug treatment. Therefore, the metabolic activity or flux in cancer cells could be a potent biomarker for cancer detection and treatment monitoring. Magnetic resonance (MR)-based sensing technologies have been developed with hyperpolarized molecules for real-time flux analysis, but they still suffer from low sensitivity and throughput. To address this limitation, we have developed an innovative miniaturized MR coil, termed micro-slab MR coil, for simultaneous analysis of metabolic flux in multiple samples. Combining this approach with hyperpolarized probes, we were able to quantify the pyruvate-to-lactate flux in two different leukemic cell lines in a non-destructive manner, simultaneously. Further, we were able to rapidly assess flux changes with drug treatment in a single hyperpolarization experiment. This new multi-sample system has the potential to transform our ability to assess metabolic dynamics at scale.
Application of Tissue Engineering in Manufacturing Absorbable Membranes to Improve the Osteopromoting Potential of Collagen
Authors: de Oliveira Júlio César Silva , Baggio Ana Maira Pereira , Benetti Luan Pier , Delamura Izabela Fornazari , Ramos Edith Umasi , Bizelli Vinícius Ferreira , Bassi Ana Paula Farnezi
Published: December 2022

The membranes are an important biomaterial that contribute to osteopromotion. This study aimed to evaluate the osteopromotive potential of collagen membranes associated with Hydroxyapatite (HA) in critical size calvaria rat’s defects. Ninety-six Albinus Wistar rats were divided into four groups: (CG) negative control: clot only (CG); positive control: porcine collagen membrane (BG); fish collagen membrane associated with HA (CP); bovine collagen membrane associated with HA (CB), analyzed at 7, 15, 30, and 60 postoperative days. At 30 days, membrane integrity was observed in the CB and fragments in the CP and BG groups were dispersed in the center of the defect. At 60 days, BG demonstrated better results with no statistical difference for the CP group (p = 0.199) and a statistically significant difference for the CB group (p = 0.013). The inflammatory profiles of the BG and CP groups were similar. Immunohistochemistry demonstrated at 60 days moderate osteopontin staining for the BG and CP groups, light staining for the CB, and intense osteocalcin staining for the BG, while the CB and CP groups demonstrated moderate staining. Microtomography revealed the highest mean bone volume (14.247 mm3) in the BG, followed by the CB (11.850 mm3), and CP (9.560 mm3) group. The collagen membranes associated with HA demonstrated an osteopromotive potential.
Cardioprotective Mechanisms against Reperfusion Injury in Acute Myocardial Infarction: Targeting Angiotensin II Receptors
Authors: Méndez-Valdés Gabriel , Pérez-Carreño Vicente , Bragato Maria Chiara , Hundahl Malthe , Chichiarelli Silvia , Saso Luciano , Rodrigo Ramón
Published: December 2022

Ischemia/reperfusion injury is a process associated with cardiologic interventions, such as percutaneous coronary angioplasty after an acute myocardial infarction. Blood flow restoration causes a quick burst of reactive oxygen species (ROS), which generates multiple organelle damage, leading to the activation of cell death pathways. Therefore, the intervention contributes to a greater necrotic zone, thus increasing the risk of cardiovascular complications. A major cardiovascular ROS source in this setting is the activation of multiple NADPH oxidases, which could result via the occupancy of type 1 angiotensin II receptors (AT1R); hence, the renin angiotensin system (RAS) is associated with the generation of ROS during reperfusion. In addition, ROS can promote the expression of NF-κΒ, a proinflammatory transcription factor. Recent studies have described an intracellular RAS pathway that is associated with increased intramitochondrial ROS through the action of isoform NOX4 of NADPH oxidase, thereby contributing to mitochondrial dysfunction. On the other hand, the angiotensin II/ angiotensin type 2 receptor (Ang II/AT2R) axis exerts its effects by counter-modulating the action of AT1R, by activating endothelial nitric oxide synthase (eNOS) and stimulating cardioprotective pathways such as akt. The aim of this review is to discuss the possible use of AT1R blockers to hamper both the Ang II/AT1R axis and the associated ROS burst. Moreover; we suggest that AT1R antagonist drugs should act synergistically with other cardioprotective agents, such as ascorbic acid, N-acetylcysteine and deferoxamine, leading to an enhanced reduction in the reperfusion injury. This therapy is currently being tested in our laboratory and has shown promising outcomes in experimental studies.
When to Return to Normal? Temporal Dynamics of Vigilance in Four Situations
Authors: Mettke-Hofmann Claudia
Published: December 2022

Vigilance is an important behaviour to monitor the environment from detecting predators to tracking conspecifics. However, little is known about how vigilance changes over time either without disturbance (vigilance decrement) or after a change occurred. The time course of vigilance can indicate how animals perceive a situation and the potential mechanism used to deal with it. I investigated the time course of vigilance in Gouldian Finches in four situations (familiar environment, two changed environments–novel object at a neutral location (exploration trial) or above the feeder (neophobia trial), novel environment). The frequency of head movements was assessed in four consecutive 15-min blocks in same sex pairs with a high frequency generally seen as indicative of high vigilance. Vigilance decreased over time in the familiar situation indicating vigilance decrement with a similar time course in the exploration trial. Vigilance was consistently high in the neophobia trial and only returned to normal in the last block. Finally, vigilance plummeted in the novel environment and did not return to normal within an hour. Results suggest that perceived threats affected vigilance and that information gathering reduced uncertainty allowing vigilance to return to normal levels but with different time courses depending on the situation.
Semi-Supervised Random Forest Methodology for Fault Diagnosis in Air-Handling Units
Authors: Ma Guofeng , Ding Haoran
Published: December 2022

Air-handling units have been widely used in indoor air conditioning and circulation in modern buildings. The data-driven FDD method has been widely used in the field of industrial roads, and has been widely welcomed because of its extensiveness and flexibility in practical applications. Under the condition of sufficient labeled data, previous studies have verified the utility and value of various supervised learning algorithms in FDD tasks. However, in practice, obtaining sufficient labeled data can be very challenging, expensive, and will consume a lot of time and manpower, making it difficult or even impractical to fully explore the potential of supervised learning algorithms. To solve this problem, this study proposes a semi-supervised FDD method based on random forest. This method adopts a self-training strategy for semi-supervised learning and has been verified in two practical applications: fault diagnosis and fault detection. Through a large number of data experiments, the influence of key learning parameters is statistically represented, including the availability of marked data, the number of iterations of maximum half-supervised learning, and the threshold of utilization of pseudo-label data. The results show that the proposed method can effectively utilize a large number of unlabeled data, improve the generalization performance of the model, and improve the diagnostic accuracy of different column categories by about 10%. The results are helpful for the development of advanced data-driven fault detection and diagnosis tools for intelligent building systems.
Optical Genome Mapping for Comprehensive Assessment of Chromosomal Aberrations and Discovery of New Fusion Genes in Pediatric B-Acute Lymphoblastic Leukemia
Authors: Gao Huixia , Xu Hanli , Wang Chanjuan , Cui Lei , Huang Xiaotong , Li Weijing , Yue Zhixia , Tian Shuo , Zhao Xiaoxi , Xue Tianlin , Xing Tianyu , Li Jun , Wang Ying , Zhang Ruidong , Li Zhigang , Wang Tianyou
Published: December 2022

Purpose: To assess the potential added value of Optical Genomic Mapping (OGM) for identifying chromosomal aberrations. Methods: We utilized Optical Genomic Mapping (OGM) to determine chromosomal aberrations in 46 children with B-cell Acute lymphoblastic leukemia ALL (B-ALL) and compared the results of OGM with conventional technologies. Partial detection results were verified by WGS and PCR. Results: OGM showed a good concordance with conventional cytogenetic techniques in identifying the reproducible and pathologically significant genomic SVs. Two new fusion genes (LMNB1::PPP2R2B and TMEM272::KDM4B) were identified by OGM and verified by WGS and RT-PCR for the first time. OGM has a greater ability to detect complex chromosomal aberrations, refine complicated karyotypes, and identify more SVs. Several novel fusion genes and single-gene alterations, associated with definite or potential pathologic significance that had not been detected by traditional methods, were also identified. Conclusion: OGM addresses some of the limitations associated with conventional cytogenomic testing. This all-in-one process allows the detection of most major genomic risk markers in one test, which may have important meanings for the development of leukemia pathogenesis and targeted drugs.
Timosaponin AIII Inhibits Migration and Invasion Abilities in Human Cervical Cancer Cells through Inactivation of p38 MAPK-Mediated uPA Expression In Vitro and In Vivo
Authors: Chien Hung-Ju , Liu Chung-Jung , Ying Tsung-Ho , Wu Pei-Ju , Wang Jiunn-Wei , Ting Yi-Hsuan , Hsieh Yi-Hsien , Wang Shih-Chiang
Published: December 2022

Cervical cancer is one of the most common gynecologic cancers globally that require novel approaches. Timosaponin AIII (TSAIII) is a steroidal saponin that displays beneficial effects in antitumor activities. However, the effect of TSAIII on human cervical cancer remains unknown. In this study, we found that TSAIII showed no influence on cell viability, cytotoxicity, cell cycle distribution and apoptosis induction in human cervical cancer cells. TSAIII was revealed to have a significant inhibitory effect on cell migration and invasion through the downregulation of invasion-related uPA expression and p38 MAPK activation in both human cervical cancer cells and cervical cancer stem cells (CCSCs), indicating that the p38 MAPK–uPA axis mediated the TSAIII-inhibited capacity of cellular migration and invasion. In a synergistic inhibition assay, a TSAIII plus p38 siRNA cotreatment revealed a greater inhibition of uPA expression, migration and invasion in human cervical cancer cells. In an immunodeficient mouse model, TSAIII significantly inhibited lung metastases from human cervical cancer SiHa cells without TSAIII-induced toxicity. These findings first revealed the inhibitory effects of TSAIII on the progression of human cervical cancer through its downregulation of p38 MAPK–uPA axis activation. Therefore, TSAIII might provide a potential strategy for auxiliary therapy in human cervical cancer.
Blinatumomab in Relapsed/Refractory Burkitt Lymphoma
Authors: Bohler Jeanne , Bacher Ulrike , Banz Yara , Stadelmann Raphael , Medinger Michael , Zander Thilo , Pabst Thomas
Published: December 2022

In patients with relapsed/refractory Burkitt lymphoma (r/r BL), overall survival (OS) is poor, and effective therapies and evidence for the best therapy are lacking. The monoclonal antibody blinatumomab may represent a novel option. However, only limited data on the use of blinatumomab in r/r BL are so far available. This multi-center, retrospective case series investigated nine patients with r/r BL treated with blinatumomab. The safety of blinatumomab was assessed with respect to frequency and severity of adverse effects (AEs) infections, cytokine release syndrome (CRS) and neurotoxicity. Progression-free survival (PFS), OS and overall response rate (ORR) were analyzed to assess efficacy. No AEs > grade 2 occurred, and AEs were generally treatable and fully reversible. The best response to blinatumomab was complete remission in 3/9 patients and partial remission in 2/9, whilst 4/9 presented with progressive disease. Median PFS and OS were 2 and 6 months, respectively, ranging from 5 days to 32 months and 11 days to 32 months, respectively. Blinatumomab treatment was a successful bridging treatment to stem cell transplantation in 3/9 patients. The response to blinatumomab varied widely, and only one patient survived longer term, but activity in patients with r/r BL was evident in some patients, with its use being safe, warranting its prospective investigation.
A Race-Specific, DNA Methylation Analysis of Aging in Normal Rectum: Implications for the Biology of Aging and Its Relationship to Rectal Cancer
Authors: Devall Matthew A. , Sun Xiangqing , Eaton Stephen , Cooper Gregory S. , Willis Joseph E. , Weisenberger Daniel J. , Casey Graham , Li Li
Published: December 2022

Approximately 90% of colorectal cancer (CRC) develop over the age of 50, highlighting the important role of aging in CRC risk. African Americans (AAs) shoulder a greater CRC burden than European Americans (EA) and are more likely to develop CRC at a younger age. The effects of aging in AA and EA normal rectal tissue have yet to be defined. Here, we performed epigenome-wide DNA methylation analysis in the first, large-scale biracial cohort of normal rectum (n = 140 samples). We identified increased epigenetic age acceleration in EA than AA rectum (p = 3.91 × 10−4) using linear regression. We also identified differentially methylated regions (DMRs) associated with chronological aging in AA and EA, separately using DMRcate. Next, a consensus set of regions associated with cancer was identified through DMR analysis of two rectal cancer cohorts. The vast majority of AA DMRs were present in our analysis of aging in rectum of EA subjects, though rates of epigenetic drift were significantly greater in AA (p = 1.94 × 10−45). However, 3.66-fold more DMRs were associated with aging in rectum of EA subjects, many of which were also associated with rectal cancer. Our findings reveal a novel relationship between race, age, DNA methylation and rectal cancer risk that warrants further investigation.
Co-Targeting Luminal B Breast Cancer with S-Adenosylmethionine and Immune Checkpoint Inhibitor Reduces Primary Tumor Growth and Progression, and Metastasis to Lungs and Bone
Authors: Mehdi Ali , Attias Mikhael , Arakelian Ani , Piccirillo Ciriaco A. , Szyf Moshe , Rabbani Shafaat A.
Published: December 2022

Breast cancer (BCa) is the most prevalent cancer in females and has a high rate of mortality, especially due to increased metastasis to skeletal and non-skeletal sites. Despite the marked clinical accomplishment of immune checkpoint inhibitor (CPI) therapy in patients with several cancers, it has had limited success in luminal subtypes of BCa. Accordingly, recent efforts have focused on combination therapy with CPI, including epigenetic modulators, to increase response rates of CPI in luminal BCa. We have previously shown that S-adenosylmethionine (SAM), the ubiquitous methyl donor, has strong anti-cancer effects in various cancers, including all subtypes of BCa. In the current study, we took a novel approach and examined the effect of CPI alone and in combination with SAM on tumor growth and metastasis in a syngeneic mouse model of luminal B BCa. We showed that SAM decreases cell proliferation, colony-formation (survival), and invasion of luminal B BCa cell lines (Eo771, R221A) in vitro. In in vivo studies, in Eo771 tumor-bearing mice, either SAM or anti-PD-1 antibody treatment alone significantly reduced tumor growth and progression, while the SAM+anti-PD-1 combination treatment had the highest anti-cancer efficacy of all groups. The SAM+anti-PD-1 combination reduced the percentage of animals with lung metastasis, as well as total metastatic lesion area, compared to control. Additionally, the SAM+anti-PD-1 combination significantly reduced the skeletal lesion area and protected tibial integrity to a greater extent than the monotherapies in an Eo771 bone metastasis model. Transcriptome analysis of Eo771 primary tumors revealed significant downregulation of pro-metastatic genes, including Matrix metalloproteinases (MMPs) and related pathways. On the other hand, CD8+ T cell infiltration, CD8+ T cell cytotoxicity (elevated granzymes), and immunostimulatory genes and pathways were significantly upregulated by the combination treatment. The results presented point to a combination of SAM with CPI as a possible treatment for luminal B BCa that should be tested in clinical studies.
Dual Biologic Therapy in Moderate to Severe Pediatric Inflammatory Bowel Disease: A Retrospective Study
Authors: Wlazło Magdalena , Meglicka Monika , Wiernicka Anna , Osiecki Marcin , Kierkuś Jarosław
Published: December 2022

Background: Inflammatory bowel diseases in children are characterized by a wide variety of symptoms and often a severe clinical course. In the treatment, we aimed to induce and maintain remission. We focused on assessing the efficacy and safety of the concomitant use of two biologic therapies including: anti-TNF (infliximab, adalimumab) vedolizumab and ustekinumab in a refractory pediatric IBD cohort. Methods: Fourteen children (nine ulcerative colitis, one ulcerative colitis/IBD-unspecified, four Crohn’s disease) with a disease duration of 5.2 (8 months–14 years) years, initiated dual therapy at an age of 11.7 (3–17) years after failure of monotherapy with a biological drug. Five patients (36%) were treated with vedolizumab/adalimumab (VDZ + ADA), five (36%) with ustekinumab/adalimumab (UST + ADA), and three (21%) with infliximab/vedolizumab (IFX + VDZ). One patient (7%) was switched from a combination of vedolizumab and adalimumab to ustekinumab and adalimumab during follow-up. Results: A clinical improvement was obtained in ten children (73%; 5 UC, 1 UC/IBD-unspecified, 4 CD) on the PCDAI/PUCAI scale after 4 months of a second biological drug being added. The median fecal calprotectin decreased from 1610 µg/g (140–10,100) to 586 µg/g (5–3410; p = 0.028) between baseline and 4 months. Conclusions: Our clinical experience suggests that dual therapy may be an option for pediatric patients with moderate and severe courses of IBD with limited therapeutic options
The Effects of Financial Stress and Household Socio-Economic Deprivation on the Malnutrition Statuses of Children under Five during the COVID-19 Lockdown in a Marginalized Region of South Punjab, Pakistan
Authors: Alam Muhammad Babar , Shahid Muhammad , Alzghoul Bashar Isam , Yang Juan , Zakar Rubeena , Malik Najma Iqbal , Bibi Asma , Tang Kun
Published: December 2022

The lockdown after the COVID-19 pandemic not only caused public health crises and income stress but also put millions at risk of food insecurity and malnutrition across the globe, especially in low and middle-income countries [LMICs]. This study evaluated the effects of financial stress and household socio-economic deprivation on the nutritional status of 1551 children under the age of five during COVID-19 in Pakistan. A self-administered questionnaire was used between November 2020 and April 2021 to collect information on age, height, children’s weight, and socio-economic status from 1152 rural households from underdeveloped regions in Punjab, Pakistan. With the help of the proportionate simple random sampling method, this study employed a model (binary logistic regression) to calculate the likelihood of malnourishment. The findings showed that the stunting, underweight, and wasting prevalence rates during COVID-19 were 58.86%, 41.89%, and 8.11%, respectively, in the Bahawalpur region. According to the binary logistic regression analysis, among the household deprivation status (HDS) categories, the risks of childhood malnutrition were lesser in HDS-2 (OR = 0.05, 95% CI: 0. 005–0.879) and HDS-3 (OR = 0.04, 95% CI: 0.008–0.193). Similar to this, within the financial stress index (FSI) categories, the children in homes with medium financial stress had reduced odds of malnutrition (OR = 0.10, 95% CI: 0.018–0.567), and the children in households with low financial stress had reduced risks of malnutrition (OR = 0.006, 95% CI: 0.005–0.061). The proposed research found that stunting and underweight increased by 17.26% and 12.29% during the COVID-19 lockdown in the Bahawalpur region. Additionally, financial stress and socio-economic deprivation strongly affected children’s nutritional statuses during the COVID-19 lockdown in the Bahawalpur region of Southern Punjab.
A 5-Year-Old Child with a Deep Neck Abscess Complicated by Laryngeal Obstruction
Authors: Di Ludovico Armando , Raso Massimiliano , Di Filippo Paola , Di Pillo Sabrina , Zappacosta Roberta , Parruti Giustino , Zingariello Pasquale , Chiarelli Francesco , Attanasi Marina
Published: December 2022

Deep neck space infections (DNSI) are defined as infections in the potential spaces and fascial planes of the neck. We show the clinical case of a retro and para-pharyngeal abscess in a healthy 5-year-old child complicated by compression and dislocation of the larynx with marked airway caliber reduction and potentially fatal extension up to the mediastinal aditus. DNSI can occur at any age and, due to its rapid progression, requires immediate treatment in children. In healthy children, concurrent abscesses in separate neck spaces are rare. DNSI recurrence should alert the physician to the possibility of a congenital problem, and if imaging fails, laryngoscopy may be the best diagnostic technique.
Deuterium Content of the Organic Compounds in Food Has an Impact on Tumor Growth in Mice
Authors: Somlyai Gábor , Nagy Lajos I. , Puskás László G. , Papp András , Kovács Beáta Z. , Fórizs István , Czuppon György , Somlyai Ildikó
Published: December 2022

Research with deuterium-depleted water (DDW) in the last two decades proved that the deuterium/hydrogen ratio has a key role in cell cycle regulation and cellular metabolism. The present study aimed to investigate the possible effect of deuterium-depleted yolk (DDyolk) alone and in combination with DDW on cancer growth in two in vivo mouse models. To produce DDyolk, the drinking water of laying hens was replaced with DDW (25 ppm) for 6 weeks, resulting in a 60 ppm D level in dried egg yolk that was used as a deuterium-depleted food additive. In one model, 4T1, a cell line with a high metastatic capacity to the lung was inoculated in the mice’s mammary pad. After three weeks of treatment with DDW and/or DDyolk, the tumor volume in the lungs was smaller in all treated groups vs. controls with natural D levels. Tumor growth and survival in mice transplanted with an MCF-7 breast cancer cell line showed that the anticancer effect of DDW was enhanced by food containing the deuterium-depleted yolk. The study confirmed the importance of the D/H ratio in consumed water and in metabolic water produced by the mitochondria while oxidizing nutrient molecules. This is in line with the concept that the initiation of cell growth requires the cells to generate a higher D/H ratio, but DDW, DDyolk, or the naturally low-D lipids in a ketogenic diet, have a significant effect on tumor growth by preventing the cells from raising the D/H ratio to the threshold.
Intraoperative 3D-US-mpMRI Elastic Fusion Imaging-Guided Robotic Radical Prostatectomy: A Pilot Study
Authors: Oderda Marco , Calleris Giorgio , D’Agate Daniele , Falcone Marco , Faletti Riccardo , Gatti Marco , Marra Giancarlo , Marquis Alessandro , Gontero Paolo
Published: December 2022

Introduction: When performing a nerve-sparing (NS) robotic radical prostatectomy (RARP), cancer location based on multiparametric MRI (mpMRI) is essential, as well as the location of positive biopsy cores outside mpMRI targets. The aim of this pilot study was to assess the feasibility of intraoperative 3D-TRUS-mpMRI elastic fusion imaging to guide RARP and to evaluate its impact on the surgical strategy. Methods: We prospectively enrolled 11 patients with organ-confined mpMRI-visible prostate cancer (PCa), histologically confirmed at transperineal fusion biopsy using Koelis Trinity. Before surgery, the 3D model of the prostate generated at biopsy was updated, showing both mpMRI lesions and positive biopsy cores, and was displayed on the Da Vinci robotic console using TilePro™ function. Results: Intraoperative 3D modeling was feasible in all patients (median of 6 min). The use of 3D models led to a major change in surgical strategy in six cases (54%), allowing bilateral instead of monolateral NS, or monolateral NS instead of non-NS, to be performed. At pathologic examination, no positive surgical margins (PSMs) were reported. Bilateral PCa presence was detected in one (9%), four (36%), and nine (81%) patients after mpMRI, biopsy, and RARP, respectively. Extracapsular extension was found in two patients (18%) even if it was not suspected at MRI. Conclusions: Intraoperative 3D-TRUS-mpMRI modeling with Koelis Trinity is feasible and reliable, helping the surgeon to maximize functional outcomes without increasing the risk of positive surgical margins. The location of positive biopsy cores must be registered in 3D models, given the rates of bilateral involvement not seen at mpMRI.
Genetic Diversity and Population Structure Derived from Body Remains of the Endangered Flightless Longhorn Beetle Iberodorcadion fuliginator in Grassland Fragments in Central Europe
Authors: Rusterholz Hans-Peter , Ursenbacher Sylvain , Weibel Urs , Coray Armin , Baur Bruno
Published: December 2022

Knowledge of patterns of genetic diversity in populations of threatened species is vital for their effective conservation. However, destructive sampling should be avoided in threatened species so as not to additionally increase the risk of local population extinction. We exclusively used beetle remains and beetles collected after death to analyze local and regional patterns of genetic variation in the endangered flightless longhorn beetle Iberodorcadion fuliginator in the border region of Switzerland, France and Germany, in grassland remnants. We extracted DNA from the beetles’ remains and genotyped 243 individuals at 6 microsatellite loci. We found moderate genetic differentiation between populations, each belonging to one of two metapopulations situated on either side of the river Rhine, but distinct genetic differentiation between populations across metapopulation. The genetic distance between populations was correlated with the geographic distance between the sites sampled. Genetic structure analysis inferred the presence of two genetic clusters. The populations in the Alsace (France) represent one cluster, together with the Swiss populations near Basel, which is separated by the river Rhine from the cluster composed of the populations in southwestern Germany. Thus, the historical separation by the river Rhine surpasses more recent effects of human-induced habitat fragmentation on the genetic differentiation in I. fuliginator.
Spectral Library of Maize Leaves under Nitrogen Deficiency Stress
Authors: Torres-Madronero Maria C. , Goez Manuel , Guzman Manuel A. , Rondon Tatiana , Carmona Pablo , Acevedo-Correa Camilo , Gomez-Ortega Santiago , Durango-Flórez Mariana , López Smith V. , Galeano July , Casamitjana Maria
Published: December 2022

Maize crops occupy an important place in world food security. However, different conditions, such as abiotic stress factors, can affect the productivity of these crops, requiring technologies that facilitate their monitoring. One such technology is spectroscopy, which measures the energy reflected and emitted by a surface along the electromagnetic spectrum. Spectral data can help to identify abiotic factors in plants, since the spectral signature of vegetation has discriminating features associated with the plant’s health condition. This paper introduces a spectral library captured on maize crops under different nitrogen-deficiency stress levels. The datasets will be of potential interest to researchers, ecologists, and agronomists seeking to understand the spectral features of maize under nitrogen-deficiency stress. The library includes three datasets captured at different growth stages of 10 tropical maize genotypes. The spectral signatures collected were in the visible to near-infrared range (450–950 nm). The data were pre-processed to reduce noise and anomalous signatures. This study presents a spectral library of the effects of nitrogen deficiency on ten maize genotypes, highlighting that some genotypes show tolerance to this type of stress at different phenological stages. Most of the evaluated genotypes showed discriminate spectral features 4–6 weeks after sowing. Higher reflectance was obtained at approximately 550 nm for the lowest nitrogen fertilization treatments. Finally, we describe some potential applications of the spectral library of maize leaves under nitrogen-deficiency stress.
Pilot Study of the Metabolomic Profile of an Athlete after Short-Term Physical Activity
Authors: Malsagova Kristina A. , Kopylov Arthur T. , Pustovoyt Vasiliy I. , Stepanov Alexander A. , Enikeev Dmitry V. , Potoldykova Natalia V. , Balakin Evgenii I. , Kaysheva Anna L.
Published: December 2022

A comprehensive analysis of indicators of the state of the body between training and recovery allows a comprehensive evaluation of various aspects of health, athletic performance, and recovery. In this pilot study, an assessment of the metabolomic profile of athletes was performed, and the immunological reaction of the athlete’s body to food before exercise and 48 h after exercise was studied. As a result, 15 amino acids and 3 hormones were identified, the plasma levels of which differed between the training and recovery states. In addition, immunological reactions or hyperreactivity to food allergens were assessed using an enzyme immunoassay. It is likely that for the athletes in the study sample, 48 h is not enough time for the complete recovery of the body.
A Temporal Window Attention-Based Window-Dependent Long Short-Term Memory Network for Multivariate Time Series Prediction
Authors: Han Shuang , Dong Hongbin
Published: December 2022

Multivariate time series prediction models perform the required operation on a specific window length of a given input. However, capturing complex and nonlinear interdependencies in each temporal window remains challenging. The typical attention mechanisms assign a weight for a variable at the same time or the features of each previous time step to capture spatio-temporal correlations. However, it fails to directly extract each time step’s relevant features that affect future values to learn the spatio-temporal pattern from a global perspective. To this end, a temporal window attention-based window-dependent long short-term memory network (TWA-WDLSTM) is proposed to enhance the temporal dependencies, which exploits the encoder–decoder framework. In the encoder, we design a temporal window attention mechanism to select relevant exogenous series in a temporal window. Furthermore, we introduce a window-dependent long short-term memory network (WDLSTM) to encode the input sequences in a temporal window into a feature representation and capture very long term dependencies. In the decoder, we use WDLSTM to generate the prediction values. We applied our model to four real-world datasets in comparison to a variety of state-of-the-art models. The experimental results suggest that TWA-WDLSTM can outperform comparison models. In addition, the temporal window attention mechanism has good interpretability. We can observe which variable contributes to the future value.
Knowledge-Enhanced Compressed Measurements for Detection of Frequency-Hopping Spread Spectrum Signals Based on Task-Specific Information and Deep Neural Networks
Authors: Liu Feng , Jiang Yinghai
Published: December 2022

The frequency-hopping spread spectrum (FHSS) technique is widely used in secure communications. In this technique, the signal carrier frequency hops over a large band. The conventional non-compressed receiver must sample the signal at high rates to catch the entire frequency-hopping range, which is unfeasible for wide frequency-hopping ranges. In this paper, we propose an efficient adaptive compressed method to measure and detect the FHSS signals non-cooperatively. In contrast to the literature, the FHSS signal-detection method proposed in this paper is achieved directly with compressed sampling rates. The measurement kernels (the non-zero coefficients in the measurement matrix) are designed adaptively, using continuously updated knowledge from the compressed measurement. More importantly, in contrast to the iterative optimizations of the measurement matrices in the literature, the deep neural networks are trained once using task-specific information optimization and repeatedly implemented for measurement kernel design, enabling efficient adaptive detection of the FHSS signals. Simulations show that the proposed method provides stably low missing detection rates, compared to the compressed detection with random measurement kernels and the recently proposed method. Meanwhile, the measurement design in the proposed method is shown to provide improved efficiency, compared to the commonly used recursive method.
From Modified Newtonian Dynamics to Superfluid Vacuum Theory
Authors: Scott Tony
Published: December 2022

Herein is a review of the essentials of Modified Newtonian Dynamics (MOND) versus dark matter models based on Superfluids for modeling galactic rotation curves. We review the successes and issues of both approaches. We then mention a recent alternative based on the Superfluid Vacuum Theory (SVT) with a nonlinear logarithmic Schrödinger equation (LogSE) which reconciles both approaches, retains the essential success of MOND and the Superfluid nature but does not necessitate the hypothesis of processes including dark matter. We conclude with the implications of this SVT alternative on quantum theory itself.
Optimization of Three Key Geometries of a Steam Ejector under Varied Primary Nozzle Geometries
Authors: Yan Jia , Li Ruixin , Wang Chen
Published: December 2022

In this paper, three key geometrical dimensions such as XL3 (constant pressure mixing chamber length), D5 (diameter of the throat of the ejector), and XL5 (length of the throat of the ejector) were separately or sequentially optimized under different lengths and angles of two sections of the primary nozzle. Furthermore, shock cluster number and shock chain length or area of low static pressure zone were used to analyze the effect of geometries on ejector performance, which is normally indicated by entrainment ratio (ER, or the ratio between the mass flow rate of secondary flow and the mass flow rate of primary flow). The results show that: (1) the improvement of ejector performance with only optimization of the primary nozzle is quite limited, in comparison, the impact of length and angle of nozzle diverging section on ejector performance is higher than that of converging section; (2) the relative sensitivity of ER to three key dimensions is much higher than that to the angles and lengths of the primary nozzle; (3) with the increase of XL3 and D5, ER needs a larger angle and smaller length of nozzle converging section; (4) the impact of key geometries on ejector performance can be analyzed with the help of shock cluster number and shock chain length or area of low static pressure zone.
Ecological Half-Life of 137Cs in Fungi
Authors: Zarubina Nataliia E. , Semak Vladislav , Burdo Oleg S. , Ponomarenko Liliia P.
Published: December 2022

The ecological half-life of 137Cs was calculated individually for four symbiotrophic fungi species (Boletus edulis, Imleria badia, Suillus luteus, Paxillus involutus) at 10 sampling sites in the Chornobyl exclusion zone and in the Kyiv region. It was found that the maximum rate of excretion of 137Cs from the fungi organisms is characteristic for the territory with the maximum levels of soil contamination, i.e., for a zone near Chornobyl Nuclear Power Plant. In areas with low 137Cs content, a slowing down of the excretion rate predominates. These results reveal different fungal response to the distinct concentration levels of 137Cs in forest ecosystems. This observation further suggests that radiocaesium can be selectively accumulated by fungi and used in their life processes. Presence of this 137Cs retention mechanism in fungi leads to a longer contamination of woody plants-symbionts.
COVID-19 Stress and Teachers Well-Being: The Mediating Role of Sense of Coherence and Resilience
Authors: Zewude Girum Tareke , Beyene Sisay Demissew , Taye Belayneh , Sadouki Fatiha , Hercz Maria
Published: December 2022

The outbreak of the COVID-19 pandemic has impacted many professions with short-, medium-, and long-term consequences. Hence, this study examined the mediating role of sense of coherence (SOC) and resilience in the relation to COVID-19 stress and teachers’ well-being (TWB). It recruited 836 teachers from Ethiopia’s higher-education institutions, of which 630 (75.4%) were men and 206 (24.6%) were women, with a mean age of 32.81 years and a standard deviation of 6.42. Findings showed that COVID-19 stress negatively predicted SOC, resilience, and TWB and that SOC and resilience positively predicted TWB. It was concluded that SOC and resilience, both together and separately, mediated the relation between COVID-19 stress and TWB. These results were discussed alongside relevant literature, and the study is found to be valuable for practitioners and researchers who seek to improve well-being using SOC and resilience as resources across teaching professions.
Experimental Investigation on the Noise Characteristics of a Squirrel-Cage Fan with Different Blade Lengths
Authors: Li Zhehong , Cao Wenbin , Ye Xinxue , Wei Yikun
Published: December 2022

Squirrel-cage fans are widely applied in air conditioning systems, and their aerodynamic noise mainly related to blade length. The aerodynamic performance and noise spectrum of squirrel-cage fans are synchronously measured in an anechoic wind tunnel. The effect of blade lengths and different geometric configurations on the noise of a squirrel-cage fan is experimentally investigated. This paper focuses on the total sound pressure level and noise spectrum characteristics at different measurement points. Noise distributions of the outlet of the forward squirrel-cage fan exhibited axial-symmetry and large differences for those of vertical direction. In lower fan positions, the noise was greater than that of the top. In particular, it was found that blades were easy to generate higher noise when their length was reduced by 31.7%. The findings suggest that the broadband noise of the squirrel-cage fan should be fully considered for noise reduction. The purpose of this work is to provide a novelty reference for the low-cost modification method of cutting blades. The results show that fans with shorter blades have lower noise and kept an excellent performance. These finding have implications for fan manufacturers.
Influence and Mechanism Study of Ultrasonic Electric Power Input on Heavy Oil Viscosity
Authors: Gao Jinbiao , Wu Pengfei , Li Chao , Xu Delong , Wang Xiuming
Published: December 2022

The reserves of heavy oil are enormous. However, its high viscosity and other characteristics make heavy oil extraction and transportation extremely difficult. Power ultrasonic (US) reforming technology on heavy oil has the advantages of environmental protection and fast results, so it is important to understand the mechanism of ultrasonic reforming. We examine the influence law of the electric power input of the US transducer on the viscosity of heavy oil. Fourier Transform Infrared Spectrometer (FTIR) and Gas Chromatography (GC) are applied to explain the changes in different functional groups, heavy components, and carbon chains before and after US irradiation. The cavitation noise method is also used to study the influences of variance in the intensity of cavitation on the viscosity of heavy oil. The results indicate that the viscosity of heavy oil first decreases, and next increases with an increase in electric power. The functional groups and chromatographic distillation also change in different forms, and with an increase in electric power, the cavitation effect is gradually enhanced. These findings suggest that it is not that the stronger the cavitation, the greater the influence on the viscosity of heavy oil.
A Review of Tariffs and Services for Smart Charging of Electric Vehicles in Europe
Authors: Hildermeier Julia , Burger Jaap , Jahn Andreas , Rosenow Jan
Published: December 2022

Smart charging of electric vehicles (EVs) is an essential approach to reduce the costs and maximise the benefits of increasing numbers of EVs being connected to the power grid. This article analyses 139 tariffs and services for smart EV charging available in Europe. It finds that while the market for smart EV charging services is growing, there is a lack of consumer information on the savings and broader environmental benefits it offers, such as integration of renewables. Offers are also unevenly distributed across the continent, resulting in unequal access to smart EV charging. The article outlines six strategies to establish framework conditions in energy markets that would address these gaps and establish smart charging as a standard means of charging, to ensure the beneficial integration of EVs into power grids.
Multi-Objective Hybrid Optimization for Optimal Sizing of a Hybrid Renewable Power System for Home Applications
Authors: Hossain Md. Arif , Ahmed Ashik , Tito Shafiqur Rahman , Ahshan Razzaqul , Sakib Taiyeb Hasan , Nengroo Sarvar Hussain
Published: December 2022

An optimal energy mix of various renewable energy sources and storage devices is critical for a profitable and reliable hybrid microgrid system. This work proposes a hybrid optimization method to assess the optimal energy mix of wind, photovoltaic, and battery for a hybrid system development. This study considers the hybridization of a Non-dominant Sorting Genetic Algorithm II (NSGA II) and the Grey Wolf Optimizer (GWO). The objective function was formulated to simultaneously minimize the total energy cost and loss of power supply probability. A comparative study among the proposed hybrid optimization method, Non-dominant Sorting Genetic Algorithm II, and multi-objective Particle Swarm Optimization (PSO) was performed to examine the efficiency of the proposed optimization method. The analysis shows that the applied hybrid optimization method performs better than other multi-objective optimization algorithms alone in terms of convergence speed, reaching global minima, lower mean (for minimization objective), and a higher standard deviation. The analysis also reveals that by relaxing the loss of power supply probability from 0% to 4.7%, an additional cost reduction of approximately 12.12% can be achieved. The proposed method can provide improved flexibility to the stakeholders to select the optimum combination of generation mix from the offered solutions.
Even Samples from the Same Waterlogged Wood Are Hygroscopically and Chemically Different by Simultaneous DVS and 2D COS-IR Spectroscopy
Authors: Han Liuyang , Han Xiangna , Liang Guoqing , Tian Xingling , Ma Fang , Sun Suqin , Yin Yafang , Xi Guanglan , Guo Hong
Published: December 2022

Waterlogged archaeological wood samples may degrade during long-term immersion in microbial-activity environments, which causes its biodegradation. Simultaneous dynamic vapor sorption (SDVS) and two-dimensional correlation infrared (2D COS-IR) spectroscopy reveal the degradation inhomogeneity of waterlogged fir wood from the Shengbeiyu shipwreck. The waterlogged and reference wood exhibit type II sorption isotherms. The equilibrium moisture contents of waterlogged archaeological fir wood from a decay region (WFD) were 22.5% higher than those of waterlogged archaeological fir wood from a sound region (WFS). WFD exhibits a higher measurable sorption hysteresis than WFS, implying greater variation in the surface moisture content in the WFD region compared to the WFS region, which may compromise the dimensional stability of the shipwreck. 2D COS-IR spectra confirmed the inhomogeneous degradation of the waterlogged wood via numerous mechanisms. The efficacy of SDVS and 2D COS-IR spectroscopy in the evaluation of the degradation state of waterlogged wood was demonstrated. This study verifies the existence of hygroscopic and chemical differences between visually similar samples from the same shipwreck.
Constructed Wetland Coupled Microbial Fuel Cell: A Clean Technology for Sustainable Treatment of Wastewater and Bioelectricity Generation
Authors: Kesarwani Shiwangi , Panwar Diksha , Mal Joyabrata , Pradhan Nirakar , Rani Radha
Published: December 2022

The availability of clean water and the depletion of non-renewable resources provide challenges to modern society. The widespread use of conventional wastewater treatment necessitates significant financial and energy expenditure. Constructed Wetland Microbial Fuel Cells (CW-MFCs), a more recent alternative technology that incorporates a Microbial Fuel Cell (MFC) inside a Constructed Wetland (CW), can alleviate these problems. By utilizing a CW’s inherent redox gradient, MFC can produce electricity while also improving a CW’s capacity for wastewater treatment. Electroactive bacteria in the anaerobic zone oxidize the organic contaminants in the wastewater, releasing electrons and protons in the process. Through an external circuit, these electrons travel to the cathode and produce electricity. Researchers have demonstrated the potential of CW-MFC technology in harnessing bio-electricity from wastewater while achieving pollutant removal at the lab and pilot scales, using both domestic and industrial wastewater. However, several limitations, such as inadequate removal of nitrogen, phosphates, and toxic organic/inorganic pollutants, limits its applicability on a large scale. In addition, the whole system must be well optimized to achieve effective wastewater treatment along with energy, as the ecosystem of the CW-MFC is large, and has diverse biotic and abiotic components which interact with each other in a dynamic manner. Therefore, by modifying important components and optimizing various influencing factors, the performance of this hybrid system in terms of wastewater treatment and power generation can be improved, making CW-MFCs a cost-effective, cleaner, and more sustainable approach for wastewater treatment that can be used in real-world applications in the future.