bluuebunny/crossref_metadata_2025 huggingface dataset:
A Nuclear Magnetic Resonance (NMR)- and Mass Spectrometry (MS)-Based Saturation Kinetics Model of a Bryophyllum pinnatum Decoction as a Treatment for Kidney Stones
Authors: Chik Candus , Larroque Anne-Laure , Zhuang Yuan , Feinstein Shane , Smith Donald L. , Andonian Sero , Ryan Aimee K. , Jean-Claude Bertrand , Gupta Indra R.
Published: May 2024

Bryophyllum pinnatum (BP) is a medicinal plant used to treat many conditions when taken as a leaf juice, leaves in capsules, as an ethanolic extract, and as herbal tea. These preparations have been chemically analyzed except for decoctions derived from boiled green leaves. In preparation for a clinical trial to validate BP tea as a treatment for kidney stones, we used NMR and MS analyses to characterize the saturation kinetics of the release of metabolites. During boiling of the leaves, (a) the pH decreased to 4.8 within 14 min and then stabilized; (b) regarding organic acids, citric and malic acid were released with maximum release time (tmax) = 35 min; (c) for glycoflavonoids, quercetin 3-O-α-L-arabinopyranosyl-(1 → 2)-α-L-rhamnopyranoside (Q-3O-ArRh), myricetin 3-O-α-L-arabinopyranosyl-(1 → 2)-α-L-rhamnopyranoside (M-3O-ArRh), kappinatoside, myricitrin, and quercitrin were released with tmax = 5–10 min; and (d) the total phenolic content (TPC) and the total antioxidant capacity (TAC) reached a tmax at 55 min and 61 min, respectively. In summary, 24 g of leaves boiled in 250 mL of water for 61 min ensures a maximal release of key water-soluble metabolites, including organic acids and flavonoids. These metabolites are beneficial for treating kidney stones because they target oxidative stress and inflammation and inhibit stone formation.
Immunological Aspects of Cancer Cell Metabolism
Authors: Ucche Sisca , Hayakawa Yoshihiro
Published: May 2024

Cancer cells adeptly manipulate their metabolic processes to evade immune detection, a phenomenon intensifying the complexity of cancer progression and therapy. This review delves into the critical role of cancer cell metabolism in the immune-editing landscape, highlighting how metabolic reprogramming facilitates tumor cells to thrive despite immune surveillance pressures. We explore the dynamic interactions within the tumor microenvironment (TME), where cancer cells not only accelerate their glucose and amino acid metabolism but also induce an immunosuppressive state that hampers effective immune response. Recent findings underscore the metabolic competition between tumor and immune cells, particularly focusing on how this interaction influences the efficacy of emerging immunotherapies. By integrating cutting-edge research on the metabolic pathways of cancer cells, such as the Warburg effect and glutamine addiction, we shed light on potential therapeutic targets. The review proposes that disrupting these metabolic pathways could enhance the response to immunotherapy, offering a dual-pronged strategy to combat tumor growth and immune evasion.
Potential Exosome Biomarkers for Parkinson’s Disease Diagnosis: A Systematic Review and Meta-Analysis
Authors: Kim Ka Young , Shin Ki Young , Chang Keun-A
Published: May 2024

Parkinson’s disease (PD) is the second most common neurodegenerative disease worldwide. Given its prevalence, reliable biomarkers for early diagnosis are required. Exosomal proteins within extracellular nanovesicles are promising candidates for diagnostic, screening, prognostic, and disease monitoring purposes in neurological diseases such as PD. This review aims to evaluate the potential of extracellular vesicle proteins or miRNAs as biomarkers for PD. A comprehensive literature search until January 2024 was conducted across multiple databases, including PubMed, EMBASE, Web of Science, and Cochrane Library, to identify relevant studies reporting exosome biomarkers in blood samples from PD patients. Out of 417 articles screened, 47 studies were selected for analysis. Among exosomal protein biomarkers, α-synuclein, tau, Amyloid β 1-42, and C-X-C motif chemokine ligand 12 (CXCL12) were identified as significant markers for PD. Concerning miRNA biomarkers, miRNA-24, miR-23b-3p, miR-195-3p, miR-29c, and mir-331-5p are promising across studies. α-synuclein exhibited increased levels in PD patients compared to control groups in twenty-one studies, while a decrease was observed in three studies. Our meta-analysis revealed a significant difference in total exosomal α-synuclein levels between PD patients and healthy controls (standardized mean difference [SMD] = 1.369, 95% confidence interval [CI] = 0.893 to 1.846, p < 0.001), although these results are limited by data availability. Furthermore, α-synuclein levels significantly differ between PD patients and healthy controls (SMD = 1.471, 95% CI = 0.941 to 2.002, p < 0.001). In conclusion, certain exosomal proteins and multiple miRNAs could serve as potential biomarkers for diagnosis, prognosis prediction, and assessment of disease progression in PD.
Corrosion Rate and Mechanism of Degradation of Chitosan/TiO2 Coatings Deposited on MgZnCa Alloy in Hank’s Solution
Authors: Krawiec Halina , Kozina Iryna , Starowicz Maria , Lekka Maria , Zanella Caterina , Fedrizzi Lorenzo , Fedel Michele , Deflorian Flavio
Published: May 2024

Overly fast corrosion degradation of biodegradable magnesium alloys has been a major problem over the last several years. The development of protective coatings by using biocompatible, biodegradable, and non-toxic material such as chitosan ensures a reduction in the rate of corrosion of Mg alloys in simulated body fluids. In this study, chitosan/TiO2 nanocomposite coating was used for the first time to hinder the corrosion rate of Mg19Zn1Ca alloy in Hank’s solution. The main goal of this research is to investigate and explain the corrosion degradation mechanism of Mg19Zn1Ca alloy coated by nanocomposite chitosan-based coating. The chemical composition, structural analyses, and corrosion tests were used to evaluate the protective properties of the chitosan/TiO2 coating deposited on the Mg19Zn1Ca substrate. The chitosan/TiO2 coating slows down the corrosion rate of the magnesium alloy by more than threefold (3.6 times). The interaction of TiO2 (NPs) with the hydroxy and amine groups present in the chitosan molecule cause their uniform distribution in the chitosan matrix. The chitosan/TiO2 coating limits the contact of the substrate with Hank’s solution.
Relationship between Plasma Lipid Profile and Cognitive Status in Early Alzheimer Disease
Authors: Peña-Bautista Carmen , Álvarez-Sánchez Lourdes , García-Lluch Gemma , Raga Luis , Quevedo Paola , Peretó Mar , Balaguer Angel , Baquero Miguel , Cháfer-Pericás Consuelo
Published: May 2024

Alzheimer disease (AD) is a heterogeneous and complex disease in which different pathophysiological mechanisms are involved. This heterogenicity can be reflected in different atrophy patterns or clinical manifestations. Regarding biochemical pathways involved in early AD, lipid metabolism plays an important role; therefore, lipid levels have been evaluated as potential AD diagnosis biomarkers, and their levels could be related to different AD clinical manifestations. Therefore, the aim of this work is to study AD lipid profiles from early AD patients and evaluate their clinical significance. For this purpose, untargeted plasma lipidomic analysis was carried out in early AD patients (n = 31) diagnosed with cerebrospinal fluid (CSF) biomarkers. Cluster analysis was carried out to define early AD subgroups according to the lipid levels. Then, the clinical significance of each lipid profile subgroup was studied, analyzing differences for other variables (cognitive status, CSF biomarkers, medication, comorbidities, age, and gender). The cluster analysis revealed two different groups of AD patients. Cluster 1 showed higher levels of plasma lipids and better cognitive status than Cluster 2. However, no differences were found for the other variables (age, gender, medication, comorbidities, cholesterol, and triglycerides levels) between both groups. Plasma lipid levels could differentiate two early AD subgroups, which showed different cognitive statuses. However, further research with a large cohort and longitudinal study evaluating the clinical evolution of these patients is required. In general, it would involve a relevant advance in the knowledge of AD pathological mechanisms, potential treatments, and precision medicine.
Spontaneous Overactivation of Xenopus Frog Eggs Triggers Necrotic Cell Death
Authors: Tokmakov Alexander A. , Teranishi Ryuga , Sato Ken-Ichi
Published: May 2024

The excessive activation of frog eggs, referred to as overactivation, can be initiated by strong oxidative stress, leading to expedited calcium-dependent non-apoptotic cell death. Overactivation also occurs spontaneously, albeit at a low frequency, in natural populations of spawned frog eggs. Currently, the cytological and biochemical events of the spontaneous process have not been characterized. In the present study, we demonstrate that the spontaneous overactivation of Xenopus frog eggs, similarly to oxidative stress- and mechanical stress-induced overactivation, is characterized by the fast and irreversible contraction of the egg’s cortical layer, an increase in egg size, the depletion of intracellular ATP, a drastic increase in the intracellular ADP/ATP ratio, and the degradation of M phase-specific cyclin B2. These events manifest in eggs in the absence of caspase activation within one hour of triggering overactivation. Importantly, substantial amounts of ATP and ADP leak from the overactivated eggs, indicating that plasma membrane integrity is compromised in these cells. The rupture of the plasma membrane and acute depletion of intracellular ATP explicitly define necrotic cell death. Finally, we report that egg overactivation can occur in the frog’s genital tract. Our data suggest that mechanical stress may be a key factor promoting egg overactivation during oviposition in frogs.
Library Screening, In Vivo Confirmation, and Structural and Bioinformatic Analysis of Pentapeptide Sequences as Substrates for Protein Farnesyltransferase
Authors: Schey Garrett L. , Hildebrandt Emily R. , Wang You , Diwan Safwan , Passetti Holly A. , Potts Gavin W. , Sprague-Getsy Andrea M. , Leoni Ethan R. , Kuebler Taylor S. , Sham Yuk Y. , Hougland James L. , Beese Lorena S. , Schmidt Walter K. , Distefano Mark D.
Published: May 2024

Protein farnesylation is a post-translational modification where a 15-carbon farnesyl isoprenoid is appended to the C-terminal end of a protein by farnesyltransferase (FTase). This process often causes proteins to associate with the membrane and participate in signal transduction pathways. The most common substrates of FTase are proteins that have C-terminal tetrapeptide CaaX box sequences where the cysteine is the site of modification. However, recent work has shown that five amino acid sequences can also be recognized, including the pentapeptides CMIIM and CSLMQ. In this work, peptide libraries were initially used to systematically vary the residues in those two parental sequences using an assay based on Matrix Assisted Laser Desorption Ionization–Mass Spectrometry (MALDI-MS). In addition, 192 pentapeptide sequences from the human proteome were screened using that assay to discover additional extended CaaaX-box motifs. Selected hits from that screening effort were rescreened using an in vivo yeast reporter protein assay. The X-ray crystal structure of CMIIM bound to FTase was also solved, showing that the C-terminal tripeptide of that sequence interacted with the enzyme in a similar manner as the C-terminal tripeptide of CVVM, suggesting that the tripeptide comprises a common structural element for substrate recognition in both tetrapeptide and pentapeptide sequences. Molecular dynamics simulation of CMIIM bound to FTase further shed light on the molecular interactions involved, showing that a putative catalytically competent Zn(II)-thiolate species was able to form. Bioinformatic predictions of tetrapeptide (CaaX-box) reactivity correlated well with the reactivity of pentapeptides obtained from in vivo analysis, reinforcing the importance of the C-terminal tripeptide motif. This analysis provides a structural framework for understanding the reactivity of extended CaaaX-box motifs and a method that may be useful for predicting the reactivity of additional FTase substrates bearing CaaaX-box sequences.
A High-Homology Region Provides the Possibility of Detecting β-Barrel Pore-Forming Toxins from Various Bacterial Species
Authors: Nagel Alexey S. , Vetrova Olesya S. , Rudenko Natalia V. , Karatovskaya Anna P. , Zamyatina Anna V. , Andreeva-Kovalevskaya Zhanna I. , Salyamov Vadim I. , Egorova Nadezhda A. , Siunov Alexander V. , Ivanova Tatiana D. , Boziev Khanafi M. , Brovko Fedor A. , Solonin Alexander S.
Published: May 2024

The pathogenicity of many bacteria, including Bacillus cereus and Staphylococcus aureus, depends on pore-forming toxins (PFTs), which cause the lysis of host cells by forming pores in the membranes of eukaryotic cells. Bioinformatic analysis revealed a region homologous to the Lys171-Gly250 sequence in hemolysin II (HlyII) from B. cereus in over 600 PFTs, which we designated as a “homologous peptide”. Three β-barrel PFTs were used for a detailed comparative analysis. Two of them—HlyII and cytotoxin K2 (CytK2)—are synthesized in Bacillus cereus sensu lato; the third, S. aureus α-toxin (Hla), is the most investigated representative of the family. Protein modeling showed certain amino acids of the homologous peptide to be located on the surface of the monomeric forms of these β-barrel PFTs. We obtained monoclonal antibodies against both a cloned homologous peptide and a 14-membered synthetic peptide, DSFNTFYGNQLFMK, as part of the homologous peptide. The HlyII, CytK2, and Hla regions recognized by the obtained antibodies, as well as an antibody capable of suppressing the hemolytic activity of CytK2, were identified in the course of this work. Antibodies capable of recognizing PFTs of various origins can be useful tools for both identification and suppression of the cytolytic activity of PFTs.
Regulatory Mechanism through Which Old Soybean Leaves Respond to Mn Toxicity Stress
Authors: Pan Yuhu , Shi Jianning , Li Jianyu , Zhang Rui , Xue Yingbin , Liu Ying
Published: May 2024

Manganese (Mn) is a heavy metal that can cause excessive Mn poisoning in plants, disrupting microstructural homeostasis and impairing growth and development. However, the specific response mechanisms of leaves to Mn poisoning have not been fully elucidated. This study revealed that Mn poisoning of soybean plants resulted in yellowing of old leaves. Physiological assessments of these old leaves revealed significant increases in the antioxidant enzymes activities (peroxidase (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT)) and elevated levels of malondialdehyde (MDA), proline, indoleacetic acid (IAA), and salicylic acid (SA), under 100 μM Mn toxicity. Conversely, the levels of abscisic acid (ABA), gibberellin 3 (GA3), and jasmonic acid (JA) significantly decreased. The Mn content in the affected leaves significantly increased, while the levels of Ca, Na, K, and Cu decreased. Transcriptome analysis revealed 2258 differentially expressed genes in the Mn-stressed leaves, 744 of which were upregulated and 1514 were downregulated; these genes included genes associated with ion transporters, hormone synthesis, and various enzymes. Quantitative RT-PCR (qRT-PCR) verification of fifteen genes confirmed altered gene expression in the Mn-stressed leaves. These findings suggest a complex gene regulatory mechanism under Mn toxicity and stress, providing a foundation for further exploration of Mn tolerance-related gene regulatory mechanisms in soybean leaves. Using the methods described above, this study will investigate the molecular mechanism of old soybean leaves’ response to Mn poisoning, identify key genes that play regulatory roles in Mn toxicity stress, and lay the groundwork for cultivating high-quality soybean varieties with Mn toxicity tolerance traits.
The Evolution of the WUSCHEL-Related Homeobox Gene Family in Dendrobium Species and Its Role in Sex Organ Development in D. chrysotoxum
Authors: Luo Xiaoting , Zheng Qinyao , He Xin , Zhao Xuewei , Zhang Mengmeng , Huang Ye , Cai Bangping , Liu Zhongjian
Published: May 2024

The WUSCHEL-related homeobox (WOX) transcription factor plays a vital role in stem cell maintenance and organ morphogenesis, which are essential processes for plant growth and development. Dendrobium chrysotoxum, D. huoshanense, and D. nobile are valued for their ornamental and medicinal properties. However, the specific functions of the WOX gene family in Dendrobium species are not well understood. In our study, a total of 30 WOX genes were present in the genomes of the three Dendrobium species (nine DchWOXs, 11 DhuWOXs, and ten DnoWOXs). These 30 WOXs were clustered into ancient clades, intermediate clades, and WUS/modern clades. All 30 WOXs contained a conserved homeodomain, and the conserved motifs and gene structures were similar among WOXs belonging to the same branch. D. chrysotoxum and D. huoshanense had one pair of fragment duplication genes and one pair of tandem duplication genes, respectively; D. nobile had two pairs of fragment duplication genes. The cis-acting regulatory elements (CREs) in the WOX promoter region were mainly enriched in the light response, stress response, and plant growth and development regulation. The expression pattern and RT-qPCR analysis revealed that the WOXs were involved in regulating the floral organ development of D. chrysotoxum. Among them, the high expression of DchWOX3 suggests that it might be involved in controlling lip development, whereas DchWOX5 might be involved in controlling ovary development. In conclusion, this work lays the groundwork for an in-depth investigation into the functions of WOX genes and their regulatory role in Dendrobium species’ floral organ development.
Characterization of Porous β-Type Tricalcium Phosphate Ceramics Formed via Physical Foaming with Freeze-Drying
Authors: Hashimoto Kazuaki , Oikawa Hiroto , Shibata Hirobumi
Published: May 2024

Porous β-tricalcium phosphate (Ca3(PO4)2; β-TCP) was prepared via freeze-drying and the effects of this process on pore shapes and sizes were investigated. Various samples were prepared by freezing β-TCP slurries above a liquid nitrogen surface at −180 °C with subsequent immersion in liquid nitrogen at −196 °C. These materials were then dried under reduced pressure in a freeze-dryer, after which they were sintered with heating. Compared with conventional heat-based drying, the resulting pores were more spherical, which increased both the mechanical strength and porosity of the β-TCP. These materials had a wide range of pore sizes from 50 to 200 µm, with the mean and median values both approximately 100 µm regardless of the freeze-drying conditions. Mercury porosimetry data showed that the samples contained small, interconnected pores with sizes of 1.24 ± 0.25 µm and macroscopic, interconnected pores of 25.8 ± 4.7 µm in size. The effects of nonionic surfactants having different hydrophilic/lipophilic balance (HLB) values on foaming and pore size were also investigated. Materials made with surfactants having lower HLB values exhibited smaller pores and lower porosity, whereas higher HLB surfactants gave higher porosity and slightly larger macropores. Even so, the pore diameter could not be readily controlled solely by adjusting the HLB value. The findings of this work indicated that high porosity (>75%) and good compressive strength (>2 MPa) can both be obtained in the same porous material and that foaming agents with HLB values between 12.0 and 13.5 were optimal.
Agastache Species (Lamiaceae) as a Valuable Source of Volatile Compounds: GC–MS Profiling and Investigation of In Vitro Antibacterial and Cytotoxic Activities
Authors: Nechita Mihaela-Ancuța , Pralea Ioana-Ecaterina , Țigu Adrian-Bogdan , Iuga Cristina-Adela , Pop Carmen Rodica , Gál Emese , Vârban Rodica , Nechita Vlad-Ionuț , Oniga Ovidiu , Toiu Anca , Benedec Daniela , Hanganu Daniela , Oniga Ilioara
Published: May 2024

Nowadays, there is an increasing interest in the study of medicinal and aromatic plants, due to their therapeutic properties that correlate with the presence of different active compounds. Agastache species (sp.) are aromatic plants that belong to the Lamiaceae family, originating from North America and East Asia. The present study aimed to evaluate the composition of essential oils (EOs) obtained from different Romanian cultivated Agastache sp. and to investigate their antibacterial and cytotoxic activities. The gas chromatography-mass spectrometry (GC–MS) screening revealed that menthone was the dominant constituent of A. foeniculum (31.58%), A. rugosa (39.60%) and A. rugosa ‘After Eight’ (39.76%) EOs, while estragole was the major constituent of A. foeniculum “Aromat de Buzău” (63.27%) and A. mexicana (41.66%) EOs. The investigation of the antiproliferative effect showed that A. rugosa and A. foeniculum “Aromat de Buzău” EOs had significant cytotoxic activity on MDA-MB-231 and HEPG2 tumour cell lines, with the most promising effect on the MDA-MB-231 breast cancer cell line for A. foeniculum “Aromat de Buzău” EO (IC50 = 203.70 ± 0.24 μg/mL). Regarding the antibacterial activity, A. rugosa EO was most active against E. coli (8.91 ± 3.27 μL/mL) and S. aureus (10.80 ± 0.00 μL/mL). To the best of our knowledge, this is the first report on the cytotoxic effect of Agastache sp. EOs on MDA-MB-231, HCT116 and HEPG2 tumour cell lines. The results of our study provide new and promising information for the subsequent in vivo study of the pharmacological properties of Agastache sp. essential oils.
Abiotic Stress Tolerance Boosted by Genetic Diversity in Plants
Authors: Cortés Andrés
Published: May 2024

Plant breeding [...]
Characteristics and Reservoir Development Model of the Unconformity Caused by Huaiyuan Movement in Bohai Bay Basin, China: A Case Study of Chengdao-Zhuanghai Buried Hill in Jiyang Depression
Authors: Liu Ruijuan , Wang Guozhi , Wang Yongshi , Hao Xuefeng , Qin Feng , Fang Xianxu , Meng Wei , Liu Gang
Published: May 2024

It is beneficial in terms of the theoretical significance and application prospects to define the structure and reservoir development model of the lower Paleozoic unconformity in the Jiyang Depression of Bohai Bay Basin, China, for oil and gas exploration of unconformity in carbonate strata. Geological and geochemical evidence shows that a regional unconformity formed during the Huaiyuan Movement in the lower Paleozoic strata of the Jiyang Depression. Along the top of the regional unconformity between the Yeli Liangjiashan Formation and Fengshan Formation, various types of karst breccia have developed, showing prominent characteristics of development and vertical karst zonation. The paleokarst zone can be divided into the vadose zone and the underflow zone, and there are apparent differences between the two zones in terms of the mode of karst activity and type of reservoir space. Primitive sedimentary microfacies, dolomitization, and supergene karstification controlled the reservoirs of the Fengshan Formation and Yeli-Liangjiashan Formation. There are significant differences in the original physical properties due to the differences in the original sedimentary microfacies. The pore development of granular dolomite of high-energy beach facies has the best reservoir performance. In the later period, the superposition of dolomitization and supergene karstification resulted in apparent differences in karst development mode, development intensity, reservoir type, and reservoir physical properties. Among them, the granular dolomite reservoir has the best physical properties and has developed a cavity-type reservoir that has a planar distribution along an unconformity surface.
A Semantic Analysis Method of Public Public Built Environment and Its Landscape Based on Big Data Technology: Kimbell Art Museum as Example
Authors: Zeng Zhongzhong , Wang Meizhu , Liu Dingyi , Yu Xuan , Zhang Bo
Published: May 2024

Based on big data, a new public space evaluation method is proposed. Using programming technology to collect visitor reviews from the travel website TripAdvisor to build a database, based on the data of 99,240 words in 1573 visitor reviews in 10 years, the connection between data and reality is established through systematic data classification and visualization. Following an assessment of the Kimbell Art Museum’s functionality, architectural design, and landscape design, along with visitor feedback, a new evaluation methodology was formulated for application to public buildings with landscapes. By utilizing the unique advantages of big data, it provides convenient and efficient analysis methods for public spaces with similar data foundations and opens the way for the optimization of the built environment in the information age.
Evaluation and Influential Factors of Urban Land Use Efficiency in Yangtze River Economic Belt
Authors: Han Dongqing , Cao Zhengxu
Published: May 2024

The study of urban land use efficiency is of great significance for optimizing the spatial allocation of urban land, thereby promoting the intensive use of urban land and the transformation of economic development modes. Taking the Yangtze River Economic Belt (YREB) as the study object, we chose the undesirable Slacks-Based Measure (SBM) model to calculate the urban land use efficiency (ULUE). Then, we utilized the spatial correlation analysis and econometric methods to discuss its spatio-temporal features and influential factors. The results show the following: (1) The urban land use efficiency in the YREB steadily improved from 2010 to 2022, but the inter-regional efficiency gap evidently increased. (2) There is an efficiency value to be found in a multi-center network structure, and it forms a “core-periphery” distribution pattern. The high-efficiency areas in the downstream and upstream regions of the YREB are gradually increasing, while the efficiency value in the midstream area remains low. (3) The urban efficiency values have strong correlation, and they are mainly “High-High agglomeration” and “Low-Low agglomeration”, and they show significant regional characteristics. (4) The economic level, industrial structure, and urbanization have obvious motivating effects on ULUE, and the positive spatial spillover effect is clear. The foreign direct investment and land finance hinder the boost of efficiency, and the latter has a negative spatial spillover role on the ULUE in the downstream cities.
Adobe Bricks of the Champagne Region (France): Characterization of a Chalky Raw Earth Construction Material
Authors: Polidori Guillaume , Aras-Gaudry Adrien , Beaumont Fabien , Bogard Fabien , Murer Sébastien , Lachi Mohammed , Maalouf Chadi , Moussa Tala , Bliard Christophe , Fronteau Gilles , Hamard Erwan
Published: May 2024

Raw earth bricks made from the soil of the Chalky Champagne region (France) have been used for at least two millennia in construction, a promising heritage in the context of reducing the carbon emissions of buildings. The present experimental study aims to measure the physical, mechanical, thermal, and hydric properties of adobes collected from a local village barn. The results show a high chalk content, estimated at 71%, and a clay content, acting as a binder, of 14%. Despite limited load-bearing capacity, these lightweight adobes are suitable for current single-story constructions, while their hydrothermal properties classify them as excellent moisture regulators for occupants. In association with other bio-sourced materials such as starch–beet pulp bricks, Chalky Champagne adobes yield promising insulating properties, and meet the criteria defined by current energy standards.
Microstructure and Hardness Characteristics of Swing-Arc SAW Hardfacing Layers
Authors: Zhu Zhengyu , Ran Maoyang , Li Xuyang , Ma Pichang , Liu Shubin , Wang Jiayou
Published: May 2024

Hot-rolled backup rolls are widely used in steel rolling and usually need to be repaired by arc hardfacing after becoming worn. However, a corrugated-groove defect commonly occurs on the roll surface due to the uneven hardness distribution in the hardfacing layers, affecting the proper usage of the roll. Accordingly, a new swing-arc submerged arc welding (SA-SAW) process is proposed to attempt to solve this drawback. The microstructure and hardness are then investigated experimentally for both SAW and SA-SAW hardfacing layers. It is revealed that a self-tempering effect occurs in the welding pass bottom and the welding pass side neighboring the former pass for both processes, refining the grain in the two areas. In all the zones, including the self-tempering zone (STZ), heat-affected zone (HAZ), and not-heat-affected zone in the welding pass, both SAW and SA-SAW passes crystallize in a type of columnar grain, where the grains are the finest in STZ and the coarsest in HAZ. In addition, the arc swing improves the microstructure homogeneity of the hardfacing layers by obviously lowering the tempering degree in HAZ while promoting the even distribution of the arc heat. Accordingly, the hardness of the SA-SAW bead overall increases and distributes more uniformly with a maximum difference of < 80 HV0.5 along the horizontal direction of the bead. This hardness difference in SA-SAW is accordingly decreased by ~38.5% compared to that of the SAW bead, further indicating the practicability of the new process.
Investigation of Bandgap Properties of a Piezoelectric Phononic Crystal Plate Based on the PDE Module in COMSOL
Authors: Liu Guoqing , Qian Denghui
Published: May 2024

Aiming to address the vibration noise problems on ships, we constructed a piezoelectric phononic crystal (PC) plate structure model, solved the governing equations of the structure using the partial differential equations module (PDE) in the finite element softwareCOMSOL6.1, and obtained the corresponding energy band structure, transmission curves, and vibration modal diagrams. The application of this method to probe the structural properties of two-dimensional piezoelectric PCs is described in detail. The calculation results obtained using this method were compared with the structures obtained using the traditional plane wave expansion method (PWE) and the finite element method (FE). The results were found to be in perfect agreement, which verified the feasibility of this method. To safely and effectively adjust the bandgap within a reasonable voltage range, this paper explored the order of magnitude of the plate thickness, the influence of the voltage on the bandgap, and the dependence between them. It was found that the smaller the order of magnitude of the plate thickness, the smaller the order of magnitude of the band in which the bandgap was located. The magnitude of the driving voltage that made the bandgap change became smaller accordingly. The new idea of attaching the PC plate to the conventional plate structure to achieve a vibration damping effect is also briefly introduced. Finally, the effects of lattice constant, plate width, and thickness on the bandgap were investigated.
Modeling and Reliability Analysis of MEMS Gyroscope Rotor Parameters under Vibrational Stress
Authors: Wang Lei , Pan Yuehong , Li Kai , He Lilong , Wang Qingyi , Wang Weidong
Published: May 2024

Vibrational environments can cause drift or changes in Micro-Electro-Mechanical System (MEMS) gyroscope rotor parameters, potentially impacting their performance. To improve the effective use of MEMS gyroscopes, this study introduced a method for evaluating the reliability of parameter degradation under vibration. We analyzed the working principle of MEMS gyroscope rotors and investigated how vibration affects their parameters. Focusing on zero bias and scale factor as key performance indicators, we developed an accelerated degradation model using the distributional assumption method. We then collected degradation data for these parameters under various vibration conditions. Using the Copula function, we established a reliability assessment approach to evaluate the degradation of the MEMS gyroscope rotor’s zero bias and scale factor under vibration, enabling the determination of reliability for these parameters. Experimental findings confirmed that increasing stress levels lead to reduced failure times and increased failure rates for MEMS gyroscope rotors, with significant changes observed in the zero bias parameter. Our evaluation method effectively characterizes changes in the reliability of the MEMS gyroscope rotor’s scale factor and zero bias over time, providing valuable information for practical applications of MEMS gyroscopes.
Synergistic Effect of Flavonoids and Metformin on Protection of the Methylglyoxal-Induced Damage in PC-12 Neuroblastoma Cells: Structure–Activity Relationship and Potential Target
Authors: Zhang Danyang , He Xiaoshi , Wang Ting , Xing Yan , Xiu Zhilong , Bao Yongming , Dong Yuesheng
Published: May 2024

Methylglyoxal-induced ROS elevation is the primary cause of neuronal damage. Metformin is a traditional hypoglycemic drug that has been reported to be beneficial to the nervous system. In this study, flavonoids were found to enhance the protective effect of metformin when added at a molar concentration of 0.5%. The structure–activity relationship (SAR) analysis indicated that ortho- substitution in the B ring, and the absence of double bonds between the 2 and 3 position combined with the gallate substitution with R configuration at the 3 position in the C ring played crucial roles in the synergistic effects, which could be beneficial for designing a combination of the compounds. Additionally, the mechanism study revealed that a typical flavonoid, EGCG, enhanced ROS scavenging and anti-apoptotic ability via the BCL2/Bax/Cyto C/Caspase-3 pathway, and synergistically inhibited the expression of GSK-3β, BACE-1, and APP in PC-12 cells when used in combination with metformin. The dose of metformin used in the combination was only 1/4 of the conventional dose when used alone. These results suggested that ROS-mediated apoptosis and the pathways related to amyloid plaques (Aβ) formation can be the targets for the synergistic neuroprotective effects of flavonoids and metformin.
Nitrogen-Centered Radicals Derived from Azidonucleosides
Authors: Reyes Yahaira , Adhikary Amitava , Wnuk Stanislaw F.
Published: May 2024

Azido-modified nucleosides have been extensively explored as substrates for click chemistry and the metabolic labeling of DNA and RNA. These compounds are also of interest as precursors for further synthetic elaboration and as therapeutic agents. This review discusses the chemistry of azidonucleosides related to the generation of nitrogen-centered radicals (NCRs) from the azido groups that are selectively inserted into the nucleoside frame along with the subsequent chemistry and biological implications of NCRs. For instance, the critical role of the sulfinylimine radical generated during inhibition of ribonucleotide reductases by 2′-azido-2′-deoxy pyrimidine nucleotides as well as the NCRs generated from azidonucleosides by radiation-produced (prehydrated and aqueous) electrons are discussed. Regio and stereoselectivity of incorporation of an azido group (“radical arm”) into the frame of nucleoside and selective generation of NCRs under reductive conditions, which often produce the same radical species that are observed upon ionization events due to radiation and/or other oxidative conditions that are emphasized. NCRs generated from nucleoside-modified precursors other than azidonucleosides are also discussed but only with the direct relation to the same/similar NCRs derived from azidonucleosides.
Determinants of Weight Status and Body, Health and Life Satisfaction in Young Adults
Authors: Suwalska Julia , Łukasik Sylwia , Cymerys Maciej , Suwalska Aleksandra , Bogdański Paweł
Published: May 2024

Health behaviors include behavioral patterns and habits that relate to health maintenance, restoration and improvement. They do not only affect the physical condition; they are also associated with life satisfaction. In our study, we focused on young adulthood, a specific lifespan period for establishing long-term health behavior patterns. The aim of the present study was to investigate depressive symptoms, lifestyle and eating behaviors and delineate their associations with overweight/obesity and body, health and life satisfaction in young adults in Poland. We enrolled 800 students (81.4% females and 18.6% males). Diet, physical activity, depressive symptoms, eating behaviors and body, health and life satisfaction were assessed. Multivariate logistic regression models were employed. Almost half of the participants in our study had at least mild symptoms of depression. Symptoms of depression significantly reduced the odds of satisfaction with body, health and life, whereas physical activity increased them. Overweight/obesity significantly reduced the odds of body and health satisfaction. In women, a history of depression and emotional eating increased the odds of being overweight/obese. The results of our study may contribute to the development of educational programs and intervention strategies for young adults.
Serum Albumin and Post-Stroke Outcomes: Analysis of UK Regional Registry Data, Systematic Review, and Meta-Analysis
Authors: Thuemmler Rosa J. , Pana Tiberiu A. , Carter Ben , Mahmood Ribeya , Bettencourt-Silva Joao H. , Metcalf Anthony K. , Mamas Mamas A. , Potter John F. , Myint Phyo K.
Published: May 2024

Hypoalbuminemia associates with poor acute ischemic stroke (AIS) outcomes. We hypothesised a non-linear relationship and aimed to systematically assess this association using prospective stroke data from the Norfolk and Norwich Stroke and TIA Register. Consecutive AIS patients aged ≥40 years admitted December 2003–December 2016 were included. Outcomes: In-hospital mortality, poor discharge, functional outcome (modified Rankin score 3–6), prolonged length of stay (PLoS) > 4 days, and long-term mortality. Restricted cubic spline regressions investigated the albumin–outcome relationship. We updated a systematic review (PubMed, Scopus, and Embase databases, January 2020–June 2023) and undertook a meta-analysis. A total of 9979 patients were included; mean age (standard deviation) = 78.3 (11.2) years; mean serum albumin 36.69 g/L (5.38). Compared to the cohort median, albumin < 37 g/L associated with up to two-fold higher long-term mortality (HRmax; 95% CI = 2.01; 1.61–2.49) and in-hospital mortality (RRmax; 95% CI = 1.48; 1.21–1.80). Albumin > 44 g/L associated with up to 12% higher long-term mortality (HRmax1.12; 1.06–1.19). Nine studies met our inclusion criteria totalling 23,597 patients. Low albumin associated with increased risk of long-term mortality (two studies; relative risk 1.57 (95% CI 1.11–2.22; I2 = 81.28)), as did low-normal albumin (RR 1.10 (95% CI 1.01–1.20; I2 = 0.00)). Strong evidence indicates increased long-term mortality in AIS patients with low or low-normal albumin on admission.
Simultaneous Delivery of Curcumin and Resveratrol via In Situ Gelling, Raft-Forming, Gastroretentive Formulations
Authors: Siripruekpong Worrawee , Praparatana Rachanida , Issarachot Ousanee , Wiwattanapatapee Ruedeekorn
Published: May 2024

Curcumin and resveratrol are polyphenolic compounds that have been shown to exhibit synergistic therapeutic properties including anti-inflammatory, anticancer, and antiulcer activities, which may be exploited for the treatment of gastric diseases. However, both compounds have poor aqueous solubility and rapid metabolism, resulting in a low oral bioavailability. In situ gelling, liquid formulations were developed to produce a gastroretentive, raft-forming delivery vehicle to improve bioavailability. Solid dispersions containing a mixture of curcumin and resveratrol with Eudragit® EPO (Cur/Res-SD) were first prepared using solvent evaporation, to improve the solubility and dissolution of the compounds. Solid dispersions of a weight ratio of 1:10 curcumin/resveratrol to Eudragit® EPO were subsequently incorporated into in situ gelling, liquid formulations based on the gelling polymers, sodium alginate (low viscosity and medium viscosity), pectin, and gellan gum, respectively. Calcium carbonate and sodium bicarbonate were included to produce carbon dioxide bubbles in the gel matrix, on exposure to gastric fluid, and to achieve flotation. Moreover, the calcium ions acted as a crosslinking agent for the hydrogels. Optimized formulations floated rapidly (<60 s) in simulated gastric fluid (pH = 1.2) and remained buoyant, resulting in the gradual release of more than 80% of the curcumin and resveratrol content within 8 h. The optimized formulation based on medium-viscosity sodium alginate exhibited enhanced cytotoxic activity toward human gastric adenocarcinoma cell lines (AGS), compared with unformulated curcumin and resveratrol compounds, and increased anti-inflammatory activity against RAW 264.7 macrophage cells compared with the NSAID, indomethacin. These findings demonstrate that in situ gelling, liquid formulations, loaded with a combination of curcumin and resveratrol in the form of solid dispersions, show potential as gastroretentive delivery systems for local and systemic effects.
Extracellular Vesicles in Breast Cancer: From Intercellular Communication to Therapeutic Opportunities
Authors: Muttiah Barathan , Ng Sook Luan , Lokanathan Yogeswaran , Ng Min Hwei , Law Jia Xian
Published: May 2024

Breast cancer, a multifaceted and heterogeneous disease, poses significant challenges in terms of understanding its intricate resistance mechanisms and devising effective therapeutic strategies. This review provides a comprehensive overview of the intricate landscape of extracellular vesicles (EVs) in the context of breast cancer, highlighting their diverse subtypes, biogenesis, and roles in intercellular communication within the tumour microenvironment (TME). The discussion spans various aspects, from EVs and stromal cells in breast cancer to their influence on angiogenesis, immune response, and chemoresistance. The impact of EV production in different culture systems, including two dimensional (2D), three dimensional (3D), and organoid models, is explored. Furthermore, this review delves into the therapeutic potential of EVs in breast cancer, presenting emerging strategies such as engineered EVs for gene delivery, nanoplatforms for targeted chemotherapy, and disrupting tumour derived EVs as a treatment approach. Understanding these complex interactions of EV within the breast cancer milieu is crucial for identifying resistance mechanisms and developing new therapeutic targets.
Rethinking Paul’s Rhetorical Intentions: An Interaction with Ryan S. Schellenberg’s Abject Joy
Authors: Clark Trevor A.
Published: May 2024

Ryan S. Schellenberg recaptures a more human version of the Apostle Paul by challenging the mainstream understandings of boasting and joy as rhetorical. This essay, with reference to the concept of “rhetorical framing”, suggests that Schellenberg is right in what he affirms but wrong in what he denies and that a “strategic” understanding of boasting and joy language in Philippians is still possible, and no less human.
Religious Utopianism: From Othering Reality to Othering People
Authors: Prosic Tamara
Published: May 2024

This paper intends to make an important contribution to the studies of religious utopianism by considering religions as comprehensive utopian systems which have an ontological and a social utopian mode. It argues that the ontological mode/utopia is related to human finality and that its fantastical content, abstractness and ontological Othering undermine the transformative powers of left religious social utopianism, while it encourages pernicious social Othering in religious fundamentalism. The article has four sections. In Section 1, it clarifies the definition of utopia on which the paper relies and the reasons for this particular choice. Section 2 discusses the religious ontological utopia and religions as utopian systems and utopian programs. Section 3 utilises E. Bloch’s considerations about concrete and abstract utopias to explain the reasons for the incapacity of politically left orientated religious utopianism to function as a revolutionary force. Finally, the Section 4 discusses the way religious fundamentalism employs social Othering as a way of defending the universality of its ontological vision against competing religious and pseudo-religious universals.
Hybrid Assessment for Strengthening Supply Chain Resilience and Sustainability: A Comprehensive Analysis
Authors: Attia El-Awady , Uddin Md Sharif
Published: May 2024

Organisations encounter a significant challenge in the globalised business landscape, and thus mitigate risk by establishing robust supply chains (SCs) networks is required. In a rapidly changing environment, gaining a competitive edge is imperative. However, the exploration of the essential factors enabling resilient and sustainable supply chain management (RSSCM) in construction projects has been lacking. This study aims to bridge this gap by identifying the enabling factors for resilient and sustainable supply chain management (SSCM). To achieve this, a survey was conducted among Egyptian engineers, involving 32 factors derived from an extensive literature review on RSSCM. The data collected were categorised into four groups, namely Organisational Knowledge and Competence, Risk Management and Security, Collaboration and Communication, and Planning Efficiency and Timing, using brainstorming techniques. Subsequently, the data were analysed utilising a novel hybrid assessment approach that combines evaluation of alternatives and ranking, employing the compromise solution-fuzzy synthetic evaluation methodology, for the first time, offering a unique approach to assessing and prioritising these categories. The findings reveal that ‘Planning Efficiency and Timing’ emerged as the highest-performing category, whereas ‘Collaboration and Communication’ performed the worth. Furthermore, our results indicate that brainstorming enabled the grouping of the enablers into four distinct categories, providing a structured framework for understanding and organising them. The integration of MARCOS and FSE offered a robust decision-making approach, proposing a resilient and comprehensive decision-support system capable of tackling intricate real-world issues. This research outcome offers building administrators valuable insights for comparing different supply chains, considering how supply chain characteristics influence resilience and risk exposure in building SCs.
Life Cycle Greenhouse Gas Emissions in Maize No-Till Agroecosystems in Southern Brazil Based on a Long-Term Experiment
Authors: Silva Guilherme Rosa da , Liska Adam J. , Bayer Cimelio
Published: May 2024

Brazilian agriculture is constantly questioned concerning its environmental impacts, particularly greenhouse gas (GHG) emissions. This research study used data from a 34-year field experiment to estimate the life cycle GHG emissions intensity of maize production for grain in farming systems under no-tillage (NT) and conventional tillage (CT) combined with Gramineae (oat) and legume (vetch) cover crops in southern Brazil. We applied the Feedstock Carbon Intensity Calculator for modeling the “field-to-farm gate” emissions with measured annual soil N2O and CH4 emissions data. For net CO2 emissions, increases in soil organic C (SOC) were applied as a proxy, where the CT combined with oat was a reference. The life cycle GHG emissions intensity for maize was negative under NT farming systems with Gramineae and legume cover crops, −0.7 and −0.1 kg CO2e kg−1 of maize, respectively. CT with oats as a cover crop had a GHG intensity of 1.0 kg CO2e kg−1 of maize and 2.2 Mg CO2e ha−1. NT with cover crops increased SOC (0.7 C Mg ha−1 yr−1, 0–100 cm) and contributed to the mitigation of life cycle GHG emissions of maize production. This research shows that NT with cover crops is a sustainable solution for farming in southern Brazil.