bluuebunny/crossref_metadata_2025 huggingface dataset:
Association of parental level of education and child factors on length-for-age indicator among socially vulnerable children aged 6–24 months from a Brazilian state using structural equation modelling
Authors: Normande Marília Moura e Mendes , da Silva Laryssa Cristiane , de Menezes Risia Cristina Egito , Florêncio Telma Maria de Menezes Toledo , Clemente Ana Paula Grotti
Published: May 2024

This cross-sectional study employs structural equation modelling (sEM) to explore both direct and indirect effects of parental level of education and child individual factors on the length-for-age outcomes in children aged 6–24 months assisted by the Bolsa Família Program in the State of Alagoas. A total of 1448 children were analysed by the sEM technique. A negative standardised direct effect (sDE) of the children’s younger age (sDE: −0·06; P = 0·017), the use of bottle feeding (sDE: −0·11; P < 0·001) and lack of a minimum acceptable diet (sDE: −0·09; P < 0·001) on the length-for-age indicator was found. Being female (SDE: 0·08; P = 0·001), a higher birth weight (SDE: 0·33; P < 0·001), being ever breastfed (sdE: 0·07; P = 0·004) and a higher level of parental education (SDE: 0·09; P < 0·001) showed a positive SDE effect on the child’s length-for-age. The model also demonstrated a negative standardised indirect effect (SIE) of the sweet beverage consumption (SIE: −0·08; P = 0·003) and a positive effect of being ever breastfed (SIE: 0·06; P = 0·017) on the child’s length-for-age through parental level of education as a mediator. This research underscores the crucial role of proper feeding practices and provides valuable insights for the development of targeted interventions, policies and programmes to improve nutritional well-being and promote adequate linear growth and development among young children facing similar challenges.
Aptrootia khaoyaiensis (Trypetheliaceae), a new corticolous lichen from the dry dipterocarp forest in central Thailand
Authors: Polyiam Wetchasart , Watthana Santi , Muangsan Nooduan , Parnmen Sittiporn , Lücking Robert
Published: May 2024

Aptrootia khaoyaiensis Polyiam & Lücking is described as new to science from dry dipterocarp forest in central Thailand, based on morphological assessment and sequence data of the mitochondrial small subunit (mtSSU). The new species is characterized by a corticolous habit, a corticate thallus, ascomata typically immersed in the thallus, with a brown to blackish ostiolar region, an inspersed hamathecium, and dark brown, muriform ascospores occurring 1–2 per ascus. Phylogenetic analyses support placement of the new species in Aptrootia; it is morphologically close to A. elatior but differs in the smaller ascospores and the inspersed hamathecium.
The natural course of binge-eating disorder: findings from a prospective, community-based study of adults
Authors: Javaras Kristin N. , Franco Victoria F. , Ren Boyu , Bulik Cynthia M. , Crow Scott J. , McElroy Susan L. , Pope Harrison G. , Hudson James I.
Published: May 2024

Background

Epidemiological data offer conflicting views of the natural course of binge-eating disorder (BED), with large retrospective studies suggesting a protracted course and small prospective studies suggesting a briefer duration. We thus examined changes in BED diagnostic status in a prospective, community-based study that was larger and more representative with respect to sex, age of onset, and body mass index (BMI) than prior multi-year prospective studies.

Methods

Probands and relatives with current DSM-IV BED (n = 156) from a family study of BED (‘baseline’) were selected for follow-up at 2.5 and 5 years. Probands were required to have BMI > 25 (women) or >27 (men). Diagnostic interviews and questionnaires were administered at all timepoints.

Results

Of participants with follow-up data (n = 137), 78.1% were female, and 11.7% and 88.3% reported identifying as Black and White, respectively. At baseline, their mean age was 47.2 years, and mean BMI was 36.1. At 2.5 (and 5) years, 61.3% (45.7%), 23.4% (32.6%), and 15.3% (21.7%) of assessed participants exhibited full, sub-threshold, and no BED, respectively. No participants displayed anorexia or bulimia nervosa at follow-up timepoints. Median time to remission (i.e. no BED) exceeded 60 months, and median time to relapse (i.e. sub-threshold or full BED) after remission was 30 months. Two classes of machine learning methods did not consistently outperform random guessing at predicting time to remission from baseline demographic and clinical variables.

Conclusions

Among community-based adults with higher BMI, BED improves with time, but full remission often takes many years, and relapse is common.
Virtual reality modelling for planning of percutaneous first step palliation in a newborn with heterotaxy syndrome
Authors: Dziewulska Aleksandra , Szeliga Judyta , Góreczny Sebastian
Published: May 2024

We report the first-stage percutaneous palliation in a newborn with a rare heterotaxy syndrome variant including interrupted inferior vena cava, partial anomalous pulmonary venous drainage, and restrictive interatrial communication. Virtual reality imaging aided visualisation, decision-making, and planning. Successful atrial septoplasty performed via the internal jugular vein and anomalous pulmonary vein was followed by stenting of ductus arteriosus.
COHOMOLOGIE DE DE RHAM DU REVÊTEMENT MODÉRÉ DE L’ESPACE DE DRINFELD
Authors: Junger Damien
Published: May 2024

Dans cet article, nous étudions la cohomologie de de Rham du premier revêtement de la tour de Drinfel’d. En particulier, nous obtenons une preuve purement locale du fait que la partie supercuspidale réalise la correspondance de Jacquet-Langlands locale pour
$\mathrm {GL}_n$
en la comparant à la cohomologie rigide de certaines variétés de Deligne-Lusztig. Les représentations obtenues sont analogues à celles qui apparaissent dans la cohomologie
$\ell $
-adique lorsqu’on oublie l’action du groupe de Weil. La preuve repose sur une généralisation d’un résultat d’excision de Grosse-Klönne et de la description explicite du premier revêtement en tant que revêtement cyclique obtenu par l’auteur dans un travail précédent.
Slow Dynamics of Hydrate Systems Revealed by a Double BSR
Authors: Fabre M. , Riboulot V. , Loncke L. , Ker S. , Ballas G. , Thomas Y. , Ion G. , Sultan N.
Published: May 2024

Determining how gas hydrate distribution evolved along continental margins in the past is essential to understanding its evolution in the future. Moreover, hydrate decomposition has been linked to several catastrophic events, including some of the largest submarine landslides on Earth and the massive release of greenhouse gases into the ocean. Offshore Romania, the presence of a second bottom‐simulating reflector (BSR) provides an opportunity to gain valuable insights into hydrate dynamics since the Last Glacial Period (LGP). We conducted transient modeling of hydrate thermodynamic stability by merging in‐situ observations with indirect assessments of sea‐bottom temperature, thermal conductivity, salinity, sedimentation rate, and sea‐level variations. We reveal a strong correlation between the BSRs and the base of the Gas Hydrate Stability Zone (GHSZ) during both the present and LGP periods. The gradual evolution of the GHSZ over the past 34 ka presented here supports a conceptual model that excludes catastrophic environmental scenarios.
Seismic Evidence for Crustal Magmatic Intrusion Beneath the Southern Part of the Eastern North American Margin
Authors: Li Cong , Yu Chunquan
Published: May 2024

The southern portion of the eastern North American margin (SENAM) is an archetypical volcanic passive margin formed during Mesozoic rifting. How past magmatic events affect the evolution of the SENAM remains an open question of fundamental importance. To better understand this question, here we construct a high‐resolution 3‐D crustal velocity model from the oceanic side to the continental interior with a combination of multimodal dispersion inversion and full‐waveform ambient noise tomography. Our new model reveals an oceanic‐continental transitional crust over a short horizonal distance of 100–150 km across the SENAM, with a local‐scale lower‐than‐surrounding velocity anomaly directly beneath the transitional crust. Furthermore, the new model shows three intra‐crustal higher‐than‐average velocity anomalies beneath the SENAM continent. We suggest that the magmatism assisted the Mesozoic rifting process to form the narrow ocean‐continent transitional crust along the coastline. The underplating of magma beneath the transitional crust led to a reduction of seismic velocity of the uppermost mantle. In addition, it is probable that the emplacement of the Central Atlantic Magmatic Province caused widespread magmatic intrusions within the continental crust of the SENAM, which were later solidified into intra‐crustal high‐velocity plutons. Our findings provide new insights into crustal modification history at the passive margin.
Stacking of Distributed Dynamic Strain Reveals Link Between Seismic Velocity Changes and the 2020 Unrest in Reykjanes
Authors: Maass Regina , Schippkus Sven , Hadziioannou Céline , Schwarz Benjamin , Jousset Philippe , Krawczyk Charlotte
Published: May 2024

In this study, we measure seismic velocity variations during two cycles of crustal inflation and deflation in 2020 on the Reykjanes peninsula (SW Iceland) by applying coda wave interferometry to ambient noise recorded by distributed dynamic strain sensing (also called DAS). We present a new workflow based on spatial stacking of raw data prior to cross‐correlation which substantially improves the spatial coherency and the time resolution of measurements. Using this approach, a strong correlation between velocity changes and ground deformation (in the vertical and horizontal direction) is revealed. Our findings may be related to the infiltration of volcanic fluids at shallow depths, even though the concurrent presence of various processes complicates the reliable attribution of observations to specific geological phenomena. Our work demonstrates how the spatial resolution of DAS can be exploited to enhance existing methodologies and overcome limitations inherent in conventional seismological data sets.
Shortwave Radiative Flux Variability Through the Lens of the Pacific Decadal Oscillation
Authors: Chtirkova Boriana , Folini Doris , Ferreira Correa Lucas , Wild Martin
Published: May 2024

The variability of the shortwave radiative fluxes at the surface and top of atmosphere (TOA) is examined in a pre‐industrial modeling setup using the Pacific Decadal Oscillation (PDO) as a possible pacemaker of atmospheric decadal‐scale variability. Within models from the Coupled Model Intercomparison Project—Phase 6, downwelling shortwave radiation at the surface, the net shortwave fluxes at the surface and TOA, as well as cloud radiative effects show remarkably similar patterns associated with the PDO. Through ensemble simulations designed with a pure PDO pattern in the North Pacific only, we show that the PDO relates to about 20%–40% of the unforced year‐to‐year variability of these shortwave fluxes over the Northern Hemispheric continents. The sea surface temperature imprint on shortwave‐flux variability over land is larger for spatially aggregated time series as compared to smaller areas, due to the blurring effect of small‐scale atmospheric noise. The surface and TOA radiative flux anomalies associated with the PDO index range of [−1.64; 1.64] are estimated to reach up to ±6 Wm−2 for North America, ∓3 Wm−2 for India and ±2 Wm−2 for Europe. We hypothesize that the redistribution of clouds in response to a North Pacific PDO anomaly can impact the South Pacific and North Atlantic SSTs.
Directional thermal emission and display using pixelated non-imaging micro-optics
Authors: Fan Ziwei , Hwang Taeseung , Lin Sam , Chen Yixin , Wong Zi Jing
Published: May 2024

Thermal radiation is intrinsically broadband, incoherent and non-directional. The ability to beam thermal energy preferentially in one direction is not only of fundamental importance, but it will enable high radiative efficiency critical for many thermal sensing, imaging, and energy devices. Over the years, different photonic materials and structures have been designed utilizing resonant and propagating modes to generate directional thermal emission. However, such thermal emission is narrowband and polarised, leading to limited thermal transfer efficiency. Here we experimentally demonstrate ultrabroadband polarisation-independent directional control of thermal radiation with a pixelated directional micro-emitter. Our compact pixelated directional micro-emitter facilitates tunable angular control of thermal radiation through non-imaging optical principles, producing a large emissivity contrast at different view angles. Using this platform, we further create a pixelated infrared display, where information is only observable at certain directions. Our pixelated non-imaging micro-optics approach can enable efficient radiative cooling, infrared spectroscopy, thermophotovoltaics, and thermal camouflaging.
Programmed multimaterial assembly by synergized 3D printing and freeform laser induction
Authors: Zheng Bujingda , Xie Yunchao , Xu Shichen , Meng Andrew C. , Wang Shaoyun , Wu Yuchao , Yang Shuhong , Wan Caixia , Huang Guoliang , Tour James M. , Lin Jian
Published: May 2024

In nature, structural and functional materials often form programmed three-dimensional (3D) assembly to perform daily functions, inspiring researchers to engineer multifunctional 3D structures. Despite much progress, a general method to fabricate and assemble a broad range of materials into functional 3D objects remains limited. Herein, to bridge the gap, we demonstrate a freeform multimaterial assembly process (FMAP) by integrating 3D printing (fused filament fabrication (FFF), direct ink writing (DIW)) with freeform laser induction (FLI). 3D printing performs the 3D structural material assembly, while FLI fabricates the functional materials in predesigned 3D space by synergistic, programmed control. This paper showcases the versatility of FMAP in spatially fabricating various types of functional materials (metals, semiconductors) within 3D structures for applications in crossbar circuits for LED display, a strain sensor for multifunctional springs and haptic manipulators, a UV sensor, a 3D electromagnet as a magnetic encoder, capacitive sensors for human machine interface, and an integrated microfluidic reactor with a built-in Joule heater for nanomaterial synthesis. This success underscores the potential of FMAP to redefine 3D printing and FLI for programmed multimaterial assembly.
A 2-year longitudinal study examining the change in psychosocial factors under the COVID-19 pandemic in Japan
Authors: Sugaya Nagisa , Yamamoto Tetsuya , Uchiumi Chigusa
Published: May 2024

To examine changes in individuals’ psychosocial variables (e.g., psychological distress, social isolation, and alcohol use) during the prolonged COVID-19 pandemic, a two-year longitudinal survey was conducted at approximately one-year intervals between May 2020 and May 2022, after the first COVID-19-related state of emergency was announced in Japan. The online survey was conducted on May 11-12, 2020 (Phase 1), June 14–20, 2021 (Phase 2), and May 13–30, 2022 (Phase 3). The survey in Phase 1 was conducted during the first emergency declaration period, the survey in Phase 2 was conducted during the third emergency declaration period, and the survey in Phase 3 was conducted at a time when there was no state of emergency but many COVID-19 positive cases. Notably, 3,892 participants responded to all three surveys. In addition to psychosocial inventories often used worldwide, survey items included lifestyle and stress management indicators related to COVID-19 and various sociodemographic items including occupation (e.g., healthcare workers) or income, history of medical treatment for mental problems, severe physical illnesses, and COVID-19.
Predicting multi-label emojis, emotions, and sentiments in code-mixed texts using an emojifying sentiments framework
Authors: Singh Gopendra Vikram , Ghosh Soumitra , Firdaus Mauajama , Ekbal Asif , Bhattacharyya Pushpak
Published: May 2024

In the era of social media, the use of emojis and code-mixed language has become essential in online communication. However, selecting the appropriate emoji that matches a particular sentiment or emotion in the code-mixed text can be difficult. This paper presents a novel task of predicting multiple emojis in English-Hindi code-mixed sentences and proposes a new dataset called SENTIMOJI, which extends the SemEval 2020 Task 9 SentiMix dataset. Our approach is based on exploiting the relationship between emotion, sentiment, and emojis to build an end-to-end framework. We replace the self-attention sublayers in the transformer encoder with simple linear transformations and use the RMS-layer norm instead of the normal layer norm. Moreover, we employ Gated Linear Unit and Fully Connected layers to predict emojis and identify the emotion and sentiment of a tweet. Our experimental results on the SENTIMOJI dataset demonstrate that the proposed multi-task framework outperforms the single-task framework. We also show that emojis are strongly linked to sentiment and emotion and that identifying sentiment and emotion can aid in accurately predicting the most suitable emoji. Our work contributes to the field of natural language processing and can help in the development of more effective tools for sentiment analysis and emotion recognition in code-mixed languages. The codes and data will be available at https://www.iitp.ac.in/~ai-nlp-ml/resources.html#SENTIMOJI to facilitate research.
GGT1 is a SNP eQTL gene involved in STAT3 activation and associated with the development of Post-ERCP pancreatitis
Authors: Furukawa Ryutaro , Kuwatani Masaki , Jiang Jing-Jing , Tanaka Yuki , Hasebe Rie , Murakami Kaoru , Tanaka Kumiko , Hirata Noriyuki , Ohki Izuru , Takahashi Ikuko , Yamasaki Takeshi , Shinohara Yuta , Nozawa Shunichiro , Hojyo Shintaro , Kubota Shimpei I. , Hashimoto Shigeru , Hirano Satoshi , Sakamoto Naoya , Murakami Masaaki
Published: May 2024

Post-ERCP pancreatitis (PEP) is an acute pancreatitis caused by endoscopic-retrograde-cholangiopancreatography (ERCP). About 10% of patients develop PEP after ERCP. Here we show that gamma-glutamyltransferase 1 (GGT1)-SNP rs5751901 is an eQTL in pancreatic cells associated with PEP and a positive regulator of the IL-6 amplifier. More PEP patients had the GGT1 SNP rs5751901 risk allele (C) than that of non-PEP patients at Hokkaido University Hospital. Additionally, GGT1 expression and IL-6 amplifier activation were increased in PEP pancreas samples with the risk allele. A mechanistic analysis showed that IL-6-mediated STAT3 nuclear translocation and STAT3 phosphorylation were suppressed in GGT1-deficient cells. Furthermore, GGT1 directly associated with gp130, the signal-transducer of IL-6. Importantly, GGT1-deficiency suppressed inflammation development in a STAT3/NF-κB-dependent disease model. Thus, the risk allele of GGT1-SNP rs5751901 is involved in the pathogenesis of PEP via IL-6 amplifier activation. Therefore, the GGT1-STAT3 axis in pancreas may be a prognosis marker and therapeutic target for PEP.
Selecting serum-free hepatocyte cryopreservation stage and storage temperature for the application of an “off-the-shelf” bioartificial liver system
Authors: Lee Ji-Hyun , Park Hey-Jung , Kim Young-A , Lee Doo-Hoon , Noh Jeong-Kwon , Jung Jong-Gab , Yang Mal Sook , Lee Jong Eun , Lee Se Hoon , Yoon Hee-Hoon , Lee Suk-Koo , Lee Sanghoon
Published: May 2024

The bioartificial liver (BAL) system can potentially rescue acute liver failure (ALF) patients by providing partial liver function until a suitable donor liver can be found or the native liver has self-regenerated. In this study, we established a suitable cryopreservation process for the development of an off-the-shelf BAL system. The viability of hepatocyte spheroids cryopreserved in liquid nitrogen was comparable to that of fresh primary hepatocyte spheroids. When hepatocyte spheroids were subjected to cryopreservation in a deep freezer, no statistically significant differences were observed in ammonia removal rate or urea secretion rate based on the cryopreservation period. However, the functional activity of the liver post-cryopreservation in a deep freezer was significantly lower than that observed following liquid nitrogen cryopreservation. Moreover, cryopreserving spheroid hydrogel beads in a deep freezer resulted in a significant decrease (approximately 30%) in both ammonia removal and urea secretion rates compared to the group cryopreserved in liquid nitrogen. The viabilities of spheroid hydrogel beads filled into the bioreactor of a BAL system were similar across all four groups. However, upon operating the BAL system for 24 h, the liver function activity was significantly higher in the group comprising hydrogel beads generated after thawing hepatocyte spheroids cryopreserved in liquid nitrogen. Consequently, the manufacturing of beads after the cryopreservation of hepatocyte spheroids is deemed the most suitable method, considering efficiency, economic feasibility, and liver function activity, for producing a BAL system.
Thiourea improves yield and quality traits of Brassica napus L. by upregulating the antioxidant defense system under high temperature stress
Authors: Ahmad Muhammad , Waraich Ejaz Ahmad , Zulfiqar Usman , Yong Jean Wan Hong , Ishfaq Muhammad , din Kaleem ul , Ullah Aman , Abbas Adeel , Awan Masood Iqbal , Moussa Ihab Mohamed , Elshikh Mohamed S.
Published: May 2024

High temperature stress influences plant growth, seed yield, and fatty acid contents by causing oxidative damage. This study investigated the potential of thiourea (TU) to mitigate oxidative stress and restoring seed oil content and quality in canola. The study thoroughly examined three main factors: (i) growth conditions—control and high temperature stress (35 °C); (ii) TU supplementation (1000 mg/L)—including variations like having no TU, water application at the seedling stage, TU application at seedling stage (BBCH Scale-39), water spray at anthesis stage, and TU application at anthesis stage (BBCH Scale-60); (iii) and two canola genotypes, 45S42 and Hiola-401, were studied separately. High temperature stress reduced growth and tissue water content, as plant height and relative water contents were decreased by 26 and 36% in 45S42 and 27 and 42% Hiola-401, respectively, resulting in a substantial decrease in seed yield per plant by 36 and 38% in 45S42 and Hiola-401. Seed oil content and quality parameters were also negatively affected by high temperature stress as seed oil content was reduced by 32 and 35% in 45S42 and Hiola-401. High-temperature stress increased the plant stress indicators like malondialdehyde, H2O2 content, and electrolyte leakage; these indicators were increased in both canola genotypes as compared to control. Interestingly, TU supplementation restored plant performance, enhancing height, relative water content, foliar chlorophyll (SPAD value), and seed yield per plant by 21, 15, 30, and 28% in 45S42; 19, 13, 26, and 21% in Hiola-401, respectively, under high temperature stress as compared to control. In addition, seed quality, seed oil content, linoleic acid, and linolenic acid were improved by 16, 14, and 22% in 45S42, and 16, 11, and 23% in Hiola-401, as compared to control. The most significant improvements in canola seed yield per plant were observed when TU was applied at the anthesis stage. Additionally, the research highlighted that canola genotype 45S42 responded better to TU applications and exhibited greater resilience against high temperature stress compared to genotype Hiola-401. This interesting study revealed that TU supplementation, particularly at the anthesis stage, improved high temperature stress tolerance, seed oil content, and fatty acid profile in two canola genotypes.
Predictive value for cerebrospinal fluid Alzheimer's disease profile of different measures of verbal episodic memory in patients with MCI
Authors: Salvadori Nicola , Torrigiani Edoardo Guido , Paoletti Federico Paolini , Chipi Elena , Montanucci Chiara , Verderosa Claudio , Siena Elisa , Fruttini Daniela , Parnetti Lucilla
Published: May 2024

Neuropsychological evidence of memory impairment represents the main feature of the clinical onset of typical Alzheimer’s disease (AD). Rey’s Auditory Verbal Learning Test (RAVLT) and Logical Memory (LM) are two tests both assessing verbal episodic memory, widely used in clinical practice. Our aim was to investigate the added value of their combined use in predicting cerebrospinal fluid (CSF) AD biomarkers positivity in a retrospective consecutive series of patients with mild cognitive impairment (MCI). 169 MCI patients were included. For all of them neuropsychological assessment and CSF analysis were available. According to CSF A/T/(N) profile, 109 were defined as MCI due to AD (A+T+), and 60 were non-AD MCI (A−T−). Logistic regression model and receiver-operating characteristic (ROC) curves were analyzed to evaluate the discriminatory power of single and combined sub-measures between AD and non-AD patients. The combination of RAVLT-del with LM could acceptably discriminate the two groups (AUC: 0.69, CI 95% 0.617–0.761, sens: 0.75, spec. 0.58, p < 0.001), while the single tests did not show sufficient discriminative performance. Our study shows that the combination of RAVLT delayed recall with LM better predicts the biological AD diagnosis (A+T+), showing a good discriminative power between MCI-AD from non-AD MCI. Since RAVLT and LM assess different components of verbal episodic memory, they should be considered as complementary, rather than interchangeable, tests.
An experimental study of foam-oil interactions for nonionic-based binary surfactant systems under high salinity conditions
Authors: Bello Ayomikun , Ivanova Anastasia , Bakulin Denis , Yunusov Timur , Rodionov Alexander , Burukhin Alexander , Cheremisin Alexey
Published: May 2024

A key factor affecting foam stability is the interaction of foam with oil in the reservoir. This work investigates how different types of oil influence the stability of foams generated with binary surfactant systems under a high salinity condition. Foam was generated with binary surfactant systems, one composed of a zwitterionic and a nonionic surfactant, and the other composed of an anionic and a nonionic surfactant. Our results showed that the binary surfactant foams investigated are more tolerant under high salinity conditions and in the presence of oil. This was visually observed in our microscopic analysis and was further attributed to an increase in apparent viscosity achieved with binary surfactant systems, compared to single surfactant foams. To understand the influence of oil on foam stability, we performed a mechanistic study to investigate how these oils interact with foams generated with binary surfactants, focusing on their applicability under high salinity conditions. The generation and stability of foam are linked to the ability of the surfactant system to solubilize oil molecules. Oil droplets that solubilize in the micelles appear to destabilize the foam. However, oils with higher molecular weights are too large to be solubilized in the micelles, hence the molecules will have less ability to be transported out of the foam, so oil seems to stabilize the foam. Finally, we conducted a multivariate analysis to identify the parameters that influenced foam stability in different oil types, using the experimental data from our work. The results showed that the oil molecular weight, interfacial tension between the foaming liquid and the oil, and the spreading coefficient are the most important variables for explaining the variation in the data. By performing a partial least square regression, a linear model was developed based on these most important variables, which can be used to predict foam stability for subsequent experiments under the same conditions as our work.
Machine vision-based autonomous road hazard avoidance system for self-driving vehicles
Authors: Qiu Chengqun , Tang Hao , Yang Yuchen , Wan Xinshan , Xu Xixi , Lin Shengqiang , Lin Ziheng , Meng Mingyu , Zha Changli
Published: May 2024

The resolution of traffic congestion and personal safety issues holds paramount importance for human’s life. The ability of an autonomous driving system to navigate complex road conditions is crucial. Deep learning has greatly facilitated machine vision perception in autonomous driving. Aiming at the problem of small target detection in traditional YOLOv5s, this paper proposes an optimized target detection algorithm. The C3 module on the algorithm’s backbone is upgraded to the CBAMC3 module, introducing a novel GELU activation function and EfficiCIoU loss function, which accelerate convergence on position loss lbox, confidence loss lobj, and classification loss lcls, enhance image learning capabilities and address the issue of inaccurate detection of small targets by improving the algorithm. Testing with a vehicle-mounted camera on a predefined route effectively identifies road vehicles and analyzes depth position information. The avoidance model, combined with Pure Pursuit and MPC control algorithms, exhibits more stable variations in vehicle speed, front-wheel steering angle, lateral acceleration, etc., compared to the non-optimized version. The robustness of the driving system's visual avoidance functionality is enhanced, further ameliorating congestion issues and ensuring personal safety.
Molecular and serological diagnosis of multiple bacterial zoonoses in febrile outpatients in Garissa County, north-eastern Kenya
Authors: Wainaina Martin , Lindahl Johanna F. , Mayer-Scholl Anne , Ufermann Christoph-Martin , Domelevo Entfellner Jean-Baka , Roesler Uwe , Roesel Kristina , Grace Delia , Bett Bernard , Al Dahouk Sascha
Published: May 2024

Bacterial zoonoses are diseases caused by bacterial pathogens that can be naturally transmitted between humans and vertebrate animals. They are important causes of non-malarial fevers in Kenya, yet their epidemiology remains unclear. We investigated brucellosis, Q-fever and leptospirosis in the venous blood of 216 malaria-negative febrile patients recruited in two health centres (98 from Ijara and 118 from Sangailu health centres) in Garissa County in north-eastern Kenya. We determined exposure to the three zoonoses using serological (Rose Bengal test for Brucella spp., ELISA for C. burnetti and microscopic agglutination test for Leptospira spp.) and real-time PCR testing and identified risk factors for exposure. We also used non-targeted metagenomic sequencing on nine selected patients to assess the presence of other possible bacterial causes of non-malarial fevers. Considerable PCR positivity was found for Brucella (19.4%, 95% confidence intervals [CI] 14.2–25.5) and Leptospira spp. (1.7%, 95% CI 0.4–4.9), and high endpoint titres were observed against leptospiral serovar Grippotyphosa from the serological testing. Patients aged 5–17 years old had 4.02 (95% CI 1.18–13.70, p-value = 0.03) and 2.42 (95% CI 1.09–5.34, p-value = 0.03) times higher odds of infection with Brucella spp. and Coxiella burnetii than those of ages 35–80. Additionally, patients who sourced water from dams/springs, and other sources (protected wells, boreholes, bottled water, and water pans) had 2.39 (95% CI 1.22–4.68, p-value = 0.01) and 2.24 (1.15–4.35, p-value = 0.02) times higher odds of exposure to C. burnetii than those who used unprotected wells. Streptococcus and Moraxella spp. were determined using metagenomic sequencing. Brucellosis, leptospirosis, Streptococcus and Moraxella infections are potentially important causes of non-malarial fevers in Garissa. This knowledge can guide routine diagnosis, thus helping lower the disease burden and ensure better health outcomes, especially in younger populations.
Adverse events reporting of XPO1 inhibitor - selinexor: a real-word analysis from FAERS database
Authors: Liu Yi , Yang Runyu , Feng Hui , Du Yue , Yang Bingyu , Zhang Mengyao , He Pengcheng , Ma Bohan , Niu Fan
Published: May 2024

As the world's first oral nuclear export inhibitor, selinexor is increasingly being used in clinical applications for malignant tumors. However, there is no extensive exploration on selinexor's adverse events (ADEs), necessitating a real-word assessment of its clinical medication safety. FAERS data (July 2019–June 2023) were searched for selinexor ADE reports across all indications. Use the system organ class (SOC) and preferred terms (PT) from the medical dictionary for regulatory activities (MedDRA) to describe, categorize, and statistic ADEs. Disproportionality analysis was employed through calculation of reporting odds ratio (ROR) and proportional reporting ratio (PRR). Based on total of 4392 selinexor related ADE reports as the primary suspect (PS), of which 2595 instances were severe outcomes. The predominant ADEs included gastrointestinal disorders, myelosuppression symptoms, and various nonspecific manifestations. 124 signals associated with selinexor ADE were detected, and 10 of these top 15 signals were not included into the instructions. Our study provides real-world evidence regarding the drug safety of selinexor, which is crucial for clinicians to safeguard patients’ health.
Sex- and cycle-dependent changes in spine density and size in hippocampal CA2 neurons
Authors: Jabra Sharif , Rietsche Michael , Muellerleile Julia , O’Leary Aet , Slattery David A. , Deller Thomas , Fellenz Meike
Published: May 2024

Sex hormones affect structural and functional plasticity in the rodent hippocampus. However, hormone levels not only differ between males and females, but also fluctuate across the female estrous cycle. While sex- and cycle-dependent differences in dendritic spine density and morphology have been found in the rodent CA1 region, but not in the CA3 or the dentate gyrus, comparable structural data on CA2, i.e. the hippocampal region involved in social recognition memory, is so far lacking. In this study, we, therefore, used wildtype male and female mice in diestrus or proestrus to analyze spines on dendritic segments from identified CA2 neurons. In basal stratum oriens, we found no differences in spine density, but a significant shift towards larger spine head areas in male mice compared to females. Conversely, in apical stratum radiatum diestrus females had a significantly higher spine density, and females in either cycle stage had a significant shift towards larger spine head areas as compared to males, with diestrus females showing the larger shift. Our results provide further evidence for the sexual dimorphism of hippocampal area CA2, and underscore the importance of considering not only the sex, but also the stage of the estrous cycle when interpreting morphological data.
Annual rhythm in immune functions of blood leucocytes in an ophidian, Natrix piscator
Authors: Singh Alka , Singh Ramesh , Parganiha Arti , Tripathi Manish Kumar
Published: May 2024

Annual variations in animal’s physiological functions are an essential strategy to deal with seasonal challenges which also vary according to the time of year. Information regarding annual adaptations in the immune-competence to cope with seasonal stressors in reptiles is scarce. The present research plan was designed to analyze the presence of circannual immune rhythms in defense responses of the leucocytes in an ophidian, Natrix piscator. Peripheral blood leucocytes were obtained, counted, and superoxide anion production, neutrophil phagocytosis, and nitrite release were tested to assess the innate immune functions. Peripheral blood lymphocytes were separated by centrifugation (utilizing density gradient) and the cell proliferation was measured. The Cosinor rhythmometry disclosed the presence of significant annual rhythms in the number of leucocytes, superoxide anion production, nitric oxide production, and proliferation of stimulated lymphocytes. The authors found that respiratory burst activity and proliferative responses of lymphocytes were crucial immune responses that showed the annual rhythm. It was summarized that the immune function of the N. piscator is a labile attribute that makes the animal competent to cope with the seasonal stressor by adjustment in the potency of response.
Development of label-free electrochemical OMP-DNA probe biosensor as a highly sensitive system to detect of citrus huanglongbing
Authors: Kazemzadeh-Beneh Hashem , Safarnejad Mohammad Reza , Norouzi Parviz , Samsampour Davood , Alavi Seyed Mehdi , Shaterreza Davood
Published: May 2024

The fabrication of the first label-free electrochemical DNA probe biosensor for highly sensitive detection of Candidatus Liberibacter asiaticus (CLas), as the causal agent of citrus huanglongbing disease, is conducted here. An OMP probe was designed based on the hybridization with its target-specific sequence in the outer membrane protein (OMP) gene of CLas. The characterization of the steps of biosensor fabrication and hybridization process between the immobilized OMP-DNA probe and the target ssDNA oligonucleotides (OMP-complementary and three mismatches OMP or OMP-mutation) was monitored using cyclic voltammetry and electrochemical impedance spectroscopy based on increasing or decreasing in the electron transfer in [Fe (CN)6]3−/4− on the modified gold electrode surface. The biosensor sensitivity indicated that the peak currents were linear over ranges from 20 to 100 nM for OMP-complementary with the detection limit of 0.026 nM (S/N = 3). The absence of any cross-interference with other biological DNA sequences confirmed a high selectivity of fabricated biosensor. Likewise, it showed good specificity in discriminating the mutation oligonucleotides from complementary target DNAs. The functional performance of optimized biosensor was achieved via the hybridization of OMP-DNA probe with extracted DNA from citrus plant infected with CLas. Therefore, fabricated biosensor indicates promise for sensitivity and early detection of citrus huanglongbing disease.
Enzymatization of mouse monoclonal antibodies to the corresponding catalytic antibodies
Authors: Hifumi Emi , Ito Yuina , Tsujita Moe , Taguchi Hiroaki , Uda Taizo
Published: May 2024

Catalytic antibodies possess a dual function that enables both antigen recognition and degradation. However, their time-consuming preparation is a significant drawback. This study developed a new method for quickly converting mice monoclonal antibodies into catalytic antibodies using site-directed mutagenesis. Three mice type monoclonal antibodies targeting hemagglutinin molecule of influenza A virus could be transformed into the catalytic antibodies by deleting Pro95 in CDR-3 of the light chain. No catalytic activity was observed for monoclonal antibodies and light chains. In contrast, the Pro95-deleted light chains exhibited a catalytic activity to cleave the antigenic peptide including the portion of conserved region of hemagglutinin molecule. The affinity of the Pro95-deleted light chains to the antigen increased approximately 100-fold compared to the wild-type light chains. In the mutants, three residues (Asp1, Ser92, and His93) come closer to the appropriate position to create the catalytic site and contributing to the enhancement of both catalytic function and immunoreactivity. Notably, the Pro95-deleted catalytic light chains could suppress influenza virus infection in vitro assay, whereas the parent antibody and the light chain did not. This strategy offers a rapid and efficient way to create catalytic antibodies from existing antibodies, accelerating the development for various applications in diagnostic and therapeutic applications.
Using isometric log-ratio in compositional data analysis for developing a groundwater pollution index
Authors: Oh Junseop , Kim Kyoung-Ho , Kim Ho-Rim , Park Sunhwa , Yun Seong-Taek
Published: May 2024

This study introduces a novel groundwater pollution index (GPI) formulated through compositional data analysis (CoDa) and robust principal component analysis (RPCA) to enhance groundwater quality assessment. Using groundwater quality monitoring data from sites impacted by the 2010–2011 foot-and-mouth disease outbreak in South Korea, CoDa uncovers critical hydrochemical differences between leachate-influenced and background groundwater. The GPI was developed by selecting key subcompositional parts (NH4
+-N, Cl-, and NO3--N) using RPCA, performing the isometric log-ratio (ILR) transformation, and normalizing the results to environmental standards, thereby providing a more precise and accurate assessment of pollution. Validated against government criteria, the GPI has shown its potential as an alternative assessment tool, with its reliability confirmed by receiver operating characteristic curve analysis. This study highlights the essential role of CoDa, especially the ILR -transformation, in overcoming the limitations of traditional statistical methods that often neglect the relative nature of hydrochemical data. Our results emphasize the utility of the GPI in significantly advancing groundwater quality monitoring and management by addressing a methodological gap in the quantitative assessment of groundwater pollution.
Discovery of seed germinating fungi (Mycetinis scorodonius) from Gastrodia elata Bl. f. glauca S. chow in Changbai Mountain and examination of their germination ability
Authors: Yang Xinyu , Gao Yugang , Li Zhaochun , Zang Pu , Zhao Yan , Liu Qun
Published: May 2024

Multi-generational asexual reproduction of Gastrodia elata Bl. will cause seedling species degeneration. Sexual reproduction of Gastrodia elata Bl. seed is an effective method to solve the problem of degeneration. The development of Gastrodia elata Bl. seeds cannot be separated from the germination fungus. However, there are few strains of germination fungus in production, and there is also the problem of species degradation in application for many years. It is very important for the sexual reproduction of Gastrodia elata Bl. to isolate more new strains of excellent germination fungus from the origin. This study used the Gastrodia elata Bl. f. glauca S. chow seeds germination vegetative propagation corms capture method to isolate its symbiotic germination fungus, and comprehensively identified the species of germination fungus by colony morphology, ITS, sporocarps regeneration and germination function, and compared the growth characteristics and germination ability with other germination fungus (Mycena purpureofusca, Mycena dendrobii and Mycena osmundicola). The germination fungus was isolated from the vegetative propagation corms of Gastrodia elata Bl. f. glauca S. chow seeds and named GYGL-1. After comprehensive identification, GYGL-1 was Mycetinis scorodonius. Compared with other germination fungus, GYGL-1 has fast germination speed, vigorous growth, and high germination ability for Gastrodia elata Bl. f. glauca S. chow seeds. Innovated the isolation method of Gastrodia elata Bl. seeds germination fungus, obtained the regenerated sporocarps of the germination fungus, and discovered that Mycetinis scorodonius has a new function of germinating Gastrodia elata Bl. f. glauca S. chow seeds, enriching the resource library of Gastrodia elata Bl. germination fungus.
Metabolic plasticity drives mismatches in physiological traits between prey and predator
Authors: Affinito Flavio , Kordas Rebecca L. , Matias Miguel G. , Pawar Samraat
Published: May 2024

Metabolic rate, the rate of energy use, underpins key ecological traits of organisms, from development and locomotion to interaction rates between individuals. In a warming world, the temperature-dependence of metabolic rate is anticipated to shift predator-prey dynamics. Yet, there is little real-world evidence on the effects of warming on trophic interactions. We measured the respiration rates of aquatic larvae of three insect species from populations experiencing a natural temperature gradient in a large-scale mesocosm experiment. Using a mechanistic model we predicted the effects of warming on these taxa’s predator-prey interaction rates. We found that species-specific differences in metabolic plasticity lead to mismatches in the temperature-dependence of their relative velocities, resulting in altered predator-prey interaction rates. This study underscores the role of metabolic plasticity at the species level in modifying trophic interactions and proposes a mechanistic modelling approach that allows an efficient, high-throughput estimation of climate change threats across species pairs.
Epitope binning for multiple antibodies simultaneously using mammalian cell display and DNA sequencing
Authors: Lin Ning , Miyamoto Kotaro , Ogawara Takumi , Sakurai Saki , Kizaka-Kondoh Shinae , Kadonosono Tetsuya
Published: May 2024

Epitope binning, an approach for grouping antibodies based on epitope similarities, is a critical step in antibody drug discovery. However, conventional methods are complex, involving individual antibody production. Here, we established Epitope Binning-seq, an epitope binning platform for simultaneously analyzing multiple antibodies. In this system, epitope similarity between the query antibodies (qAbs) displayed on antigen-expressing cells and a fluorescently labeled reference antibody (rAb) targeting a desired epitope is analyzed by flow cytometry. The qAbs with epitope similar to the rAb can be identified by next-generation sequencing analysis of fluorescence-negative cells. Sensitivity and reliability of this system are confirmed using rAbs, pertuzumab and trastuzumab, which target human epidermal growth factor receptor 2. Epitope Binning-seq enables simultaneous epitope evaluation of 14 qAbs at various abundances in libraries, grouping them into respective epitope bins. This versatile platform is applicable to diverse antibodies and antigens, potentially expediting the identification of clinically useful antibodies.
Manganese self-boosting hollow nanoenzymes with glutathione depletion for synergistic cancer chemo-chemodynamic therapy
Authors: Cai Xinyi , Cai Deng , Wang Xiaozhen , Zhang Dou , Qiu Long , Diao Zhenying , Liu Yong , Sun Jianbo , Cui Daxiang , Liu Yanlei , Yin Ting
Published: May 2024

Metal nanoenzymes (HMnO2@CDDP) that can degrade to produce Mn2+ exhibit Fenton-like, GSH-peroxidase-like activity. Treatment with nanoenzymes increased intratumor Pt-DNA content, leading to the up-regulation of γ-H2Aχ and an increase in tumor damage.
Balanced generation of holes and superoxide radicals on Bi25CoO40 sillenite photocatalysts for rapid synergetic degradation of quinolone antibiotics
Authors: Sun Hao , Wang Chen , Shao Yunhang , Zhang Yaning , Chen Chaofeng , Liu Hongyan , Dou Shuai , Xu Jing , Zhang Ying , Lou Yang , Wang Hui , Zhu Yongfa , Pan Chengsi
Published: May 2024

The internal electric field in nanosheets induces the balanced generation of holes and superoxide radicals, which synergistically degrade ciprofloxacin rapidly.
Interfacial analysis of the ion-transport process controlling the steady-state current in a two-phase electrodeposition system using polyelectrolyte membranes
Authors: Yamada Shunsuke , Takashima Yohei , Tsuruoka Takaaki , Akamatsu Kensuke
Published: May 2024

An interfacial model has been developed for describing ion transport kinetics at the membrane/solution interface in the new solid-electrodeposition system.
Accelerating metal nanoparticle exsolution by exploiting tolerance factor of perovskite stannate
Authors: Lee Yujeong , Yoon Daseob , Nam Yeon-Seo , Yu Sangbae , Lim Chaesung , Sim Hyeji , Park Yunkyu , Han Jeong Woo , Choi Si-Young , Son Junwoo
Published: May 2024

In situ exsolution have been actively performed to deliver oxide nanocomposites with metal nanoparticles. In our work, a new ingredient was unveiled to boost nickel metal exsolution on the oxide surface by increasing the octahedron distortion.
Site-selective benzylic C–H hydroxylation in electron-deficient azaheterocycles
Authors: Kaur Milanpreet , Cooper Julian C. , Van Humbeck Jeffrey F.
Published: May 2024

This report describes the use of iodine(III) to site-selectively hydroxylate adjacent to electron-deficient heterocycles such as pyridine and pyrimidine.