bluuebunny/crossref_metadata_2025 huggingface dataset:
Integration of Genomic and Clinical Retrospective Data to Predict Endometrioid Endometrial Cancer Recurrence
Authors: Gonzalez-Bosquet Jesus , Gabrilovich Sofia , McDonald Megan E. , Smith Brian J. , Leslie Kimberly K. , Bender David D. , Goodheart Michael J. , Devor Eric
Published: December 2022

Endometrial cancer (EC) incidence and mortality continues to rise. Molecular profiling of EC promises improvement of risk assessment and treatment selection. However, we still lack robust and accurate models to predict those at risk of failing treatment. The objective of this pilot study is to create models with clinical and genomic data that will discriminate patients with EC at risk of disease recurrence. We performed a pilot, retrospective, case–control study evaluating patients with EC, endometrioid type: 7 with recurrence of disease (cases), and 55 without (controls). RNA was extracted from frozen specimens and sequenced (RNAseq). Genomic features from RNAseq included transcriptome expression, genomic, and structural variation. Feature selection for variable reduction was performed with univariate ANOVA with cross-validation. Selected variables, informative for EC recurrence, were introduced in multivariate lasso regression models. Validation of models was performed in machine-learning platforms (ML) and independent datasets (TCGA). The best performing prediction models (out of >170) contained the same lncRNA features (AUC of 0.9, and 95% CI: 0.75, 1.0). Models were validated with excellent performance in ML platforms and good performance in an independent dataset. Prediction models of EC recurrence containing lncRNA features have better performance than models with clinical data alone.
New Butyroside D from Argan Press Cake Possess Anti-Melanogenesis Effect via MITF Downregulation in B16F10 and HEM Cells
Authors: Bouhoute Meryem , Amen Yhiya , Bejaoui Meriem , Mizushima Aprill Kee Oliva , Shimizu Kuniyoshi , Isoda Hiroko
Published: December 2022

Hyperpigmentation is a skin condition where patches of skin become darker in color due to excess melanin production upon UV exposure leading to melasma, which are lentigines or post inflammatory hyperpigmentation that psychologically affecting a great number of people. The present study investigates the anti-melanogenic effect of Butyroside D and the underling mechanism. After the confirmation of the non-cytotoxic effect of Butyroside D on B16F10 cells, we proceeded with analyzing the impact of the treatment at low and high concentration (i.e., 0.2 μM and 2 μM) using gene profiling analysis and examined the differentiation in gene expression. Our results identify cyclic adenosine monophosphate (cAMP), Wnt/β-catenin and Mitogen-Activated Protein Kinase (MAPK) signaling pathways to be downregulated upon treatment with Butyroside D. These pathways were targeted to further validate the effect of Butyroside D on membrane receptors melanocortin 1 receptor (MC1R) and receptor tyrosine kinase (c-Kit), related microphthalmia-associated transcription factor (MITF) and consequently tyrosinase (TYR), and tyrosine-related protein-1 (TYRP-1) that were all shown to be downregulated and, therefore, leading to the repression of melanin biosynthesis. Finally, the anti-melanogenic effect of Butyroside D was confirmed on human epidermal melanocytes (HEM) cells by inhibiting the activation of cAMP pathway generally mediated through α-melanocyte-stimulating hormone (α-MSH) and MC1R. Overall, this study suggests the potential applicability of this purified compound for the prevention of hyperpigmentation conditions.
Correction: Wu et al. Homologous Drought-Induced 19 Proteins, PtDi19-2 and PtDi19-7, Enhance Drought Tolerance in Transgenic Plants. Int. J. Mol. Sci. 2022, 23, 3371
Authors: Wu Caijuan , Lin Miao , Chen Feng , Chen Jun , Liu Shifan , Yan Hanwei , Xiang Yan
Published: December 2022

The authors wish to make the following corrections to this paper [...]
Evaluation of Phosphine Resistance in Populations of Sitophilus oryzae, Oryzaephilus surinamensis and Rhyzopertha dominica in the Czech Republic
Authors: Aulicky Radek , Stejskal Vaclav , Frydova Barbora , Athanassiou Christos
Published: December 2022

Phosphine is globally the most widely adopted fumigant for the control of storage pests. Recently, an increase in the frequency of stored-product pest resistance has been observed with significant geographical and interspecific variations. In this context, there are available data for the occurrence of resistant populations from America, Asia, Africa, and Australia, but there are few data in the case of Europe. Therefore, the aim of this work was to evaluate phosphine efficacy in important beetle pests of stored products, i.e., Sitophilus oryzae (L.), Oryzaephilus surinamensis (L.), and Rhyzopertha dominica (F.) sampled from the Czech Republic, using a rapid diagnostic test that is based on the speed to knockdown after exposure. Apart from the standard laboratory populations, which were used as the controls, we tested 56 field populations of these three species, collected in Czech farm grain stores. The survey revealed that 57.1% of the tested field populations were classified as phosphine-susceptible, based on the knockdown method used. However, profound variations among species and populations were recorded. The species with the highest percentage of resistant populations was R. dominica (71.4% of the populations; resistance coefficient 0.5–4.1), followed by S. oryzae (57.1% of the populations; resistance coefficient 0.8–6.9), and O. surinamensis (9.5% of the populations; resistance coefficient 0.5–2.9). Regarding the intra-population variability in response to phosphine (slope of the knockdown time regression), the laboratory and slightly resistant populations of all species were homogenous, whereas the most resistant populations were strongly heterogeneous. Our data show that the occurrence of resistance in the Czech Republic is relatively widespread and covers a wide range of species, necessitating the need for the adoption of an action plan for resistance mitigation.
New Method for the Solidification of High-Concentration Radioactive Borate Solution by Cement-Based Materials
Authors: Ma Haosen , Yuan Sensen , Geng Haining , Li Qiu
Published: December 2022

Cement is widely used for the solidification of low- and intermediate-level radioactive waste materials. Radioactive borate solution with a high concentration of boron is one of the main radioactive wastes produced in nuclear stations. It is difficult to solidify this solution by using cement because borate has a great inhibitory effect on the cement hydration process. In this study, the hydration kinetics, strength, durability, phase assemblage, and transportation and transformation of the silicon of the paste that blended Portland cement with 5 M borate solution were investigated. After the addition of sodium hydroxide and sodium metasilicate to the paste, the cement hydration process was restarted, and the 28-days strength of samples met the requirements of the Chinese standard. The mechanism of overcoming the retardation of cement hydration by the borate solution was attributed to the formation of calcium metaborate, ettringite, portlandite, and calcium silicate hydrate with the restarting of cement hydration, without the formation of ulexite.
Adherence to Long-Acting Inhaler Use for Initial Treatment and Acute Exacerbation of Chronic Obstructive Pulmonary Disease: A Retrospective Cohort Study
Authors: Suh Hee-Sook , Chang Min-Seok , Yu Iseul , Park Sunmin , Lee Ji-Ho , Lee Seok Jeong , Lee Won-Yeon , Yong Suk Joong , Kim Sang-Ha
Published: December 2022

We aimed to determine the effect of long-acting inhaler use adherence on acute exacerbations in treatment-naïve patients with chronic obstructive pulmonary disease (COPD) using claims data from the Korean Health Insurance Review and Assessment Service from July 2015–December 2016. Patients with COPD aged ≥ 40 years who used long-acting inhalers were enrolled and observed for 6 months. Medication adherence was determined by the medication possession ratio (MPR); patients were categorized to adherence (MPR ≥ 80%) and non-adherence (MPR < 80%) groups. Ultimately, 3959 patients were enrolled: 60.4% and 39.6% in the adherence and non-adherence groups, respectively. The relative risk of acute exacerbation in the non-adherence group was 1.58 (95% confidence interval [CI] 1.25–1.99) compared with the adherence group. The adjusted logistic regression analysis revealed a relative risk of acute exacerbation in the non-adherence vs. adherence group of 1.68 (95% CI 1.32–2.14) regarding the number of inhalers used. Poor adherence to long-acting inhalers influenced increased acute exacerbation rates among patients with COPD. The acute exacerbation of COPD risk requiring hospitalization or ED visits was high in the non-adherence group, suggesting that efforts to improve medication adherence may help reduce COPD exacerbations even in the initial management of treatment-naïve patients.
Comprehensive Analysis of a Tricycle Structure with a Steering System for Improvement of Driving Properties While Cornering
Authors: Blatnický Miroslav , Dižo Ján , Molnár Denis , Suchánek Andrej
Published: December 2022

This paper focuses on the development, theoretical and experimental research on the structural units of an unconventional three-wheeled vehicle. The vehicle is designed in order to increase the stability when cornering in a low curvature radius. Current research work describes solutions to increase the cornering stability of either conventional three-wheeled vehicles or, more rarely, unconventional vehicles designed on the basis of complex wheel-tilting mechatronics. Thus, there is a gap in research in respect of consideration of a stability-enhancing mechanism for three-wheeled vehicles based on a combination of tilting and deflection of the front steered wheel in the course of cornering. This paper then compares the stability of a three-wheeled vehicle with one steered wheel in front and two wheels in the rear (1F2R) in conventional and unconventional designs. A particular linear formula for the stability of the three-wheeled vehicle in cornering is derived. This study further deals with the design of the frame intended to hold the unconventional steering mechanism of the front wheel of the vehicle, on the one hand, from the theoretical integrity point of view using CAD-, FEM- and MBS-based software and, on the other hand, from the experimental point of view by determining the multiaxial fatigue life of the test specimens. These were made from the frame structural material and loaded with an equivalent load (bending-torsion) corresponding to the real load of the frame in operation. It was discovered that the designed patented front wheel steering mechanism increased the passing speed by 19% in comparison with a conventional vehicle at the minimum possible radius of a corner. The designed vehicle meets the safety conditions in terms of frame integrity and load-bearing capacity. The vehicle frame is designed with respect to the fatigue life of the material, the results of which are presented in the work. The material employed for manufacturing the frame is aluminum alloy type EN AW6063, which makes the frame lightweight and strong.
Influence of the Polymerization Modes on the Methacrylic Acid Release from Dental Light-Cured Materials—In Vitro Study
Authors: Lehmann Anna , Nijakowski Kacper , Drożdżyńska Agnieszka , Przybylak Martyna , Woś Patryk , Surdacka Anna
Published: December 2022

The study focuses on the problem of lowering the pH around a composite filling concerning the polymerization modes and methacrylic acid release, which may affect not only the oral health but also the whole organism. A total of 90 specimens (30 of each: Filtek Bulk Fill, Evetric and Riva LC) were placed in 90 sterile hermetic polyethene containers with saline and incubated at 37 °C. Ten samples of each material were light-cured for 40 s with one of the three curing modes: full power mode (FPM), ramping mode (RM) and pulse mode (PM). The pH and methacrylic acid release evaluation were performed at the following time points: after 2 h and after 3, 7, 21 and 42 days from the specimen preparation. Regardless of light-curing mode, all used materials were characterized by a gradual elevation in methacrylic acid concentration. Only for Filtek Bulk Fill, increased methacrylic acid release was closely associated with lower pH. The choice of the polymerization mode has no significant influence on the methacrylic acid release. However, further research about composite light-curing is necessary to create the procedure algorithm, reducing the local and systemic complications associated with composite fillings.
Improving the Performance of Solution−Processed Quantum Dot Light−Emitting Diodes via a HfOx Interfacial Layer
Authors: Jeong Jun Hyung , Kim Min Gye , Ma Jin Hyun , Park Min Ho , Ha Hyoun Ji , Kang Seong Jae , Maeng Min-Jae , Kim Young Duck , Park Yongsup , Kang Seong Jun
Published: December 2022

One of the major obstacles in the way of high−performance quantum dot light−emitting diodes (QLEDs) is the charge imbalance arising from more efficient electron injection into the emission layer than the hole injection. In previous studies, a balanced charge injection was often achieved by lowering the electron injection efficiency; however, high performance next−generation QLEDs require the hole injection efficiency to be enhanced to the level of electron injection efficiency. Here, we introduce a solution−processed HfOx layer for the enhanced hole injection efficiency. A large amount of oxygen vacancies in the HfOx films creates gap states that lower the hole injection barrier between the anode and the emission layer, resulting in enhanced light−emitting characteristics. The insertion of the HfOx layer increased the luminance of the device to 166,600 cd/m2, and the current efficiency and external quantum efficiency to 16.6 cd/A and 3.68%, respectively, compared with the values of 63,673 cd/m2, 7.37 cd/A, and 1.64% for the device without HfOx layer. The enhanced light−emitting characteristics of the device were elucidated by X−ray photoelectron, ultra−violet photoelectron, and UV−visible spectroscopy. Our results suggest that the insertion of the HfOx layer is a useful method for improving the light−emitting properties of QLEDs.
Refinement of the Magnesium–Aluminium Alloy Microstructure with Zirconium
Authors: Rapiejko Cezary , Mikusek Dominik , Januszewicz Bartłomiej , Kubiak Krzysztof J. , Pacyniak Tadeusz
Published: December 2022

The magnesium–aluminium alloy AZ91 was inoculated with zirconium to refine the microstructure. Six different concentrations of zirconium content were tested, ranging from 0.1 to 0.6 wt %, and compared to the baseline AZ91 alloy without modification. Melted metal was poured into a preheated ceramic mould and the temperature was measured and recorded during the solidification. The derivative and thermal analysis (DTA) was performed to compare the crystallisation dynamics. Formed microstructure was analysed using an optical microscope, scanning electron microscopy (SEM-EDX) and energy dispersive X-ray spectrometry (XRD). The chemical composition was measured using an arc spectrometer. The time of solidification was shortened for the samples with a concentration of zirconium 0.3 wt % and the microstructure was refined. The level of grain refinement remained below 10% and the grain shape was changed to more spherical shapes. Both the primary magnesium and eutectic phases were modified. However, at a low concentration of zirconium (0.1 and 0.2 wt %), the primary grain size was increased. Therefore, the optimal zirconium concentration was 0.3 wt %. Larger concentrations (0.4 to 0.6 wt %) did not provide any additional benefit. Theoretical analysis showed that some Al3Zr intermetallic phases can form, which was confirmed on the derivate curve of the thermal analysis, and SEM-EDS and XRD analyses.
Special Issue: Advanced Nanocomposite Materials Based on Graphene Oxide/Reduced Graphene Oxide: Potential Applications and Perspectives
Authors: Palomba Mariano , Longo Angela
Published: December 2022

In recent years, graphene oxide (GO) and reduced graphene oxide (r-GO) have received much attention as precursors of graphene-like 2D nanomaterials [...]
Influence of Polyformaldehyde Monofilament Fiber on the Engineering Properties of Foamed Concrete
Authors: Mydin Md Azree Othuman , Abdullah Mohd Mustafa Al Bakri , Mohd Nawi Mohd Nasrun , Yahya Zarina , Sofri Liyana Ahmad , Baltatu Madalina Simona , Sandu Andrei Victor , Vizureanu Petrica
Published: December 2022

Foamed concrete is considered a green building material, which is porous in nature. As a result, it poses benefits such as being light in self-weight, and also has excellent thermal insulation properties, environmental safeguards, good fire resistance performance, and low cost. Nevertheless, foamed concrete has several disadvantages such as low strength, a large amount of entrained air, poor toughness, and being a brittle material, all of which has restricted its usage in engineering and building construction. Hence, this study intends to assess the potential utilization of polypropylene fibrillated fiber (PFF) in foamed concrete to enhance its engineering properties. A total of 10 mixes of 600 and 1200 kg/m3 densities were produced by the insertion of four varying percentages of PFF (1%, 2%, 3%, and 4%). The properties assessed were splitting tensile, compressive and flexural strengths, workability, porosity, water absorption, and density. Furthermore, the correlations between the properties considered were also evaluated. The outcomes reveal that the foamed concrete mix with 4% PFF attained the highest porosity, with approximately 13.9% and 15.9% for 600 and 1200 kg/m3 densities in comparison to the control specimen. Besides, the mechanical properties (splitting tensile, compressive and flexural strengths) increased steadily with the increase in the PFF percentages up to the optimum level of 3%. Beyond 3%, the strengths reduced significantly due to poor PFF dispersal in the matrix, leading to a balling effect which causes a degraded impact of scattering the stress from the foamed concrete vicinity to another area of the PFF surface. This exploratory investigation will result in a greater comprehension of the possible applications of PFF in LFC. It is crucial to promote the sustainable development and implementation of LFC materials and infrastructures.
The Synergistic Inhibitions of Tungstate and Molybdate Anions on Pitting Corrosion Initiation for Q235 Carbon Steel in Chloride Solution
Authors: Yang Huanggen , Zhang Pei , Nie Guochao , Zhou Yong
Published: December 2022

In this work, the synergistic inhibitions of tungstate (WO42−) and molybdate (MoO42−) anions, including role and mechanism, on the initiation of pitting corrosion (PC) for Q235 carbon steel in chloride (Cl−) solution were investigated with electrochemical and surface techniques. The pitting potential (Ep) of the Q235 carbon steel in WO42− + MoO42- + Cl− solution was more positive than that in WO42− + Cl− or MoO42− + Cl− solution; at each Ep, both peak potential and affected region of active pitting sites in WO42− + MoO42− + Cl− solution were smaller than those in WO42− + Cl− or MoO42− + Cl− solution. WO42− and MoO42− showed a synergistic role to inhibit the PC initiation of the Q235 carbon steel in Cl− solution, whose mechanism was mainly attributed to the influences of two anions on passive film. Besides iron oxides and iron hydroxides, the passive film of the Q235 carbon steel formed in WO42− + Cl−, MoO42− + Cl−, or WO42− + MoO42− + Cl− solution was also composed of FeWO4 plus Fe2(WO4)3, Fe2(MoO4)3, or Fe2(WO4)3 plus Fe2(MoO4)3, respectively. The film resistance and the defect quantity for Fe2(WO4)3 plus Fe2(MoO4)3 film were larger and smaller than those for FeWO4 plus Fe2(WO4)3 film and Fe2(MoO4)3 film, respectively; for the inhibition of PC initiation, Fe2(WO4)3 plus Fe2(MoO4)3 film provided better corrosion resistance to Q235 carbon steel than FeWO4 plus Fe2(WO4)3 film and Fe2(MoO4)3 film did.
Leonotis nepetifolia Flower Bud Extract Mediated Green Synthesis of Silver Nanoparticles, Their Characterization, and In Vitro Evaluation of Biological Applications
Authors: Nagaraja Shashiraj Kariyellappa , Niazi Shaik Kalimulla , Bepari Asmatanzeem , Assiri Rasha Assad , Nayaka Sreenivasa
Published: December 2022

Biosynthesis of silver nanoparticles (AgNPs) using the green matrix is an emerging trend and is considered green nanotechnology because it involves a simple, low-cost, and environmentally friendly process. The present research aimed to synthesize silver nanoparticles from a Leonotis nepetifolia (L.) R.Br. flower bud aqueous extract, characterize these nanoparticles, and perform in vitro determination of their biological applications. UV-Vis spectra were used to study the characterization of biosynthesized L. nepetifolia-flower-bud-mediated AgNPs (LnFb-AgNPs); an SPR absorption maximum at 418 nm confirmed the formation of LnFb-AgNPs. The presumed phytoconstituents subjected to reduction in the silver ions were revealed by FTIR analysis. XRD, TEM, EDS, TGA, and zeta potential with DLS analysis revealed the crystalline nature, particle size, elemental details, surface charge, thermal stability, and spherical shape, with an average size of 24.50 nm. In addition, the LnFb-AgNPs were also tested for antimicrobial activity and exhibited a moderate zone of inhibition against the selected pathogens. Concentration-dependent antioxidant activity was observed in the DPPH assay. Further, the cytotoxicity increased proportionate to the increasing concentration of the biosynthesized LnFb-AgNPs with a maximum effect at 200 μg/mL by showing the inhibition cell viability percentages and an IC50 of 35.84 μg/mL. Subsequently, the apoptotic/necrotic potential was determined using Annexin V/Propidium Iodide staining by the flow cytometry method. Significant early and late apoptosis cell populations were observed in response to the pancreatic ductal adenocarcinoma (PANC-1) cell line, as demonstrated by the obtained results. In conclusion, the study’s findings suggest that the LnFb-AgNPs could serve as remedial agents in a wide range of biomedical applications.
Growth Features of Bi2Te3Sb1.5 Films on Polyimide Substrates Obtained by Pulsed Laser Deposition
Authors: Shupenev Alexander E. , Melnik Svetlana L. , Korshunov Ivan S. , Karpoukhin Sergey D. , Sazonkin Stanislav G. , Grigor’yants Alexander G.
Published: December 2022

Thermoelectric materials in the form of thin films are used to create a wide variety of sensors and devices. The efficiency of these devices depends on the quality and efficiency of the thermoelectric materials obtained in the form of thin films. Earlier, we demonstrated that it is possible to obtain high-performance Bi2Te3Sb1.5 films less than 1 μm thick on polyimide substrates by using the PLD method, and determined optimal growth conditions. In the current work, the relationship between growth conditions and droplet fraction on the surface, microstructure, grain size, film thickness and chemical composition was studied. A power factor of 5.25 μW/cm×K2 was achieved with the reduction of droplet fraction on the film surface to 0.57%. The dependencies of the film thickness were studied, and the effect of the thickness on the efficiency of the material is shown. The general trend in the growth dynamics for Bi2Te3Sb1.5 films we obtained is the reduction of crystalline size with Pressure-Temperature (PT) criterion. The results of our work also show the possibility of a significant reduction of droplet phase with simultaneous management of crystalline features and thermoelectric efficiency of Bi2Te3Sb1.5 films grown on polyimide substrates by varying growth conditions.
Preface to the Special Issue on “Optimization Theory and Applications”
Authors: Bűrmen Árpád , Tuma Tadej
Published: December 2022

Optimization algorithms are an irreplaceable core component of many contemporary tools used in science and engineering [...]
Functional Properties of Dunaliella salina and Its Positive Effect on Probiotics
Authors: Hyrslova Ivana , Krausova Gabriela , Mrvikova Iva , Stankova Barbora , Branyik Tomas , Malinska Hana , Huttl Martina , Kana Antonin , Doskocil Ivo
Published: December 2022

The unicellular green microalga Dunaliella is a potential source of a wide range of nutritionally important compounds applicable to the food industry. The aim of this study was to assess the effect of Dunaliella salina dried biomass on the growth and adherence of 10 strains of Lactobacillus, Lacticaseibacillus, and Bifidobacterium. The immunomodulatory, antioxidant, and cytotoxic effects of D. salina on human peripheral mononuclear cells and simulated intestinal epithelial cell lines Caco-2 and HT-29 were evaluated. Furthermore, the hypocholesterolemic effects of the microalgae on lipid metabolism in rats fed a high-fat diet were analyzed. The addition of D. salina biomass had a positive effect on the growth of nine out of 10 probiotics and promoted the adherence of three bifidobacteria strains to human cell lines. The antioxidant and immunomodulatory properties of D. salina were concentration-dependent. The inflammatory cytokines (TNF-α and IL-6) were significantly increased following Dunaliella stimulation at the lowest concentration (0.5% w/v). Eight week supplementation of D. salina to the diet of hypercholesteromic rats significantly decreased the serum concentrations of LDL-C, VLDL, IDL-B, and IDL-C. D. salina is not cytotoxic in intestinal cell models; it promotes adherence of selected bifidobacteria, it affords immunomodulatory and antioxidant effects, and its addition to diets may help decrease atherosclerosis risk factors.
Microstructure and Corrosion Behavior of AISI 316 Steel Layers Deposited on AISI 347 Steel Substrate by Laser Metal Deposition
Authors: Ligabo Isabela A. , Braga Vagner , Ferreira Caroline C. A. , Siqueira Rafael H. M. , Lourenço Julio C. , Abdalla Antonio J. , Lima Milton S. F.
Published: December 2022

The aim of this work was to evaluate the porosity, microstructure, hardness, and electrochemical behavior of AISI 316 steel layers deposited on an AISI 347 steel substrate using the LMD process. Depositions of two, four, and six layers with a 0.5 mm height for each layer were performed at a speed of 375 mm/min, a power of 250 W, a focal distance of 5 mm, and without overlapping laser tracks. The results showed epitaxial growth of the deposited layers in relation to the substrate and a predominantly austenitic microstructure with ferrite as the substrate. The deposited layers presented a dendritic microstructure with a mean porosity of 4.5%. The porosity decreased as the number of deposited layers increased, affecting the pitting corrosion resistance. The sample with six deposited layers showed greater pitting corrosion resistance, whereas the corrosion current speeds were similar for the studied samples. Vickers hardness tests showed that the hardness decreased as the distance from the substrate increased, and the hardness decreased close to the remelted regions.
Extraction and Recovery of Metals from Spent HDS Catalysts: Lab- and Pilot-Scale Results of the Overall Process
Authors: Xhaferaj Nertil , Ferella Francesco
Published: December 2022

The present study proposes an overall recycling process for spent hydrodesulfurization (HDS) catalysts. The process put together stages already known in the technical literature, tested again with samples coming from the roasting stage in a pilot kiln, which is the most limiting stage of metal recovery from spent catalysts. These catalysts contain valuable metals like cobalt (Co), molybdenum (Mo), nickel (Ni), and vanadium (V). In particular, one Co-Mo catalyst was treated in order to optimize the roasting step (time, soda ash, and temperature) at a pilot scale and thus maximize the extraction yield of molybdenum (Mo) and vanadium (V). In particular, a dry Co-Mo catalyst was used. After roasting at 700 °C for 2.5 h, the best conditions, the catalysts underwent water leaching, separating Mo and V from Co and the alumina carrier, which remained in the solid residue. The pregnant solution was treated to remove arsenic (As) and phosphorus (P), representing the main impurities for producing steel alloys. V was precipitated as NH4Cl, and further calcined to obtain commercial-grade V2O5, whereas Mo was recovered as molybdic acid by further precipitation at a pH of around one. Thus, molybdic acid was calcined and converted into commercial-grade MoO3 by calcination. The hydrometallurgical section was tested on a lab scale. The total recovery yield was nearly 61% for Mo and 68% for V, respectively, compared with their initial concentration in the spent Co-Mo catalysts.
Metabolomics and Lipidomics Signatures of Insulin Resistance and Abdominal Fat Depots in People Living with Obesity
Authors: Koay Yen Chin , Coster Adelle C. F. , Chen Daniel L. , Milner Brad , Batarseh Amani , O’Sullivan John F. , Greenfield Jerry R. , Samocha-Bonet Dorit
Published: December 2022

The liver, skeletal muscle, and adipose tissue are major insulin target tissues and key players in glucose homeostasis. We and others have described diverse insulin resistance (IR) phenotypes in people at risk of developing type 2 diabetes. It is postulated that identifying the IR phenotype in a patient may guide the treatment or the prevention strategy for better health outcomes in populations at risk. Here, we performed plasma metabolomics and lipidomics in a cohort of men and women living with obesity not complicated by diabetes (mean [SD] BMI 36.0 [4.5] kg/m2, n = 62) to identify plasma signatures of metabolites and lipids that align with phenotypes of IR (muscle, liver, or adipose tissue) and abdominal fat depots. We used 2-step hyperinsulinemic-euglycemic clamp with deuterated glucose, oral glucose tolerance test, dual-energy X-ray absorptiometry and abdominal magnetic resonance imaging to assess muscle-, liver- and adipose tissue- IR, beta cell function, body composition, abdominal fat distribution and liver fat, respectively. Spearman’s rank correlation analyses that passed the Benjamini–Hochberg statistical correction revealed that cytidine, gamma-aminobutyric acid, anandamide, and citrate corresponded uniquely with muscle IR, tryptophan, cAMP and phosphocholine corresponded uniquely with liver IR and phenylpyruvate and hydroxy-isocaproic acid corresponded uniquely with adipose tissue IR (p < 7.2 × 10−4). Plasma cholesteryl sulfate (p = 0.00029) and guanidinoacetic acid (p = 0.0001) differentiated between visceral and subcutaneous adiposity, while homogentisate correlated uniquely with liver fat (p = 0.00035). Our findings may help identify diverse insulin resistance and adiposity phenotypes and enable targeted treatments in people living with obesity.
The Effect of Diet on the Cardiac Circadian Clock in Mice: A Systematic Review
Authors: Paula Ana , Resende Letícia , Jardim Isabela , Coelho Bianca , de Coutinho Miranda Denise , Portes Alexandre , Teles Maria , Castrucci Ana Maria , Isoldi Mauro
Published: December 2022

Circadian rhythms play important roles in regulating physiological and behavioral processes. These are adjusted by environmental cues, such as diet, which acts by synchronizing or attenuating the circadian rhythms of peripheral clocks, such as the liver, intestine, pancreas, white and brown adipose tissue, lungs, kidneys, as well as the heart. Some studies point to the influence of diet composition, feeding timing, and dietary restriction on metabolic homeostasis and circadian rhythms at various levels. Therefore, this systematic review aimed to discuss studies addressing the effect of diet on the heart clock in animal models and, additionally, the chronodisruption of the clock and its relation to the development of cardiovascular disorders in the last 15 years. A search was conducted in the PubMed, Scopus, and Embase databases. The PRISMA guide was used to construct the article. Nineteen studies met all inclusion and exclusion criteria. In summary, these studies have linked the circadian clock to cardiovascular health and suggested that maintaining a robust circadian system may reduce the risks of cardiometabolic and cardiovascular diseases. The effect of time-of-day-dependent eating on the modulation of circadian rhythms of the cardiac clock and energy homeostasis is notable, among its deleterious effects predominantly in the sleep (light) phase and/or at the end of the active phase.
A Leaky-Wave Analysis of Resonant Bessel-Beam Launchers: Design Criteria, Practical Examples, and Potential Applicationsat Microwave and Millimeter-Wave Frequencies
Authors: Negri Edoardo , Fuscaldo Walter , Burghignoli Paolo , Galli Alessandro
Published: December 2022

Resonant Bessel-beam launchers are low-cost, planar, miniaturized devices capable of focusing electromagnetic radiation in a very efficient way in various frequency ranges, with recent increasing interest for microwave and millimeter-wave applications (i.e., 3–300 GHz). In recent years, various kinds of launchers have appeared, with different feeding mechanisms (e.g., coaxial probes, resonant slots, or loop antennas), field polarization (radial, azimuthal, and longitudinal), and manufacturing technology (axicon lenses, radial waveguides, or diffraction gratings). In this paper, we review the various features of these launchers both from a general electromagnetic background and a more specific leaky-wave interpretation. The latter allows for deriving a useful set of design rules that we here show to be applicable to any type of launcher, regardless its specific realization. Practical examples are discussed, showing a typical application of the proposed design workflow, along with a possible use of the launchers in a modern context, such as that of wireless power transfer at 90 GHz.
Characteristics and Controlling Factors of Natural Fractures in Continental Tight-Oil Shale Reservoir
Authors: Fu Xiaofei , Gong Lei , Su Xiaocen , Liu Bo , Gao Shuai , Yang Jianguo , Qin Xinnan
Published: December 2022

Natural fracture growth plays an important role in shale-oil enrichment. Systematically investigating fracture features and their controlling factors in shale-oil reservoirs is essential for accurately predicting fracture distribution. The controlling factors of fracture distribution in the continental shale of the Qingshankou Formation in the Songliao Basin, China, were systematically analyzed based on the quantitative fracture characterization of outcrops and cores. Strata-confined fractures, throughgoing fractures, bedding-parallel fractures, and stylolites can be observed in the Qingshankou shale reservoir in the study area. Fracture distribution is not only controlled by internal factors, e.g., mineral composition, mechanical stratigraphy, and lithofacies, but also by external factors, e.g., faults and abnormally high pressure readings. Mineral composition is the primary factor governing fracture development, and it not only controls fracture abundance, but it also affects fracture filling and effectiveness. Mechanical stratigraphy determines the spatial morphology and developmental pattern of a fracture. Fractures are well-developed in brittle strata, with fracture spacing being proportional to bed thickness. Lithofacies can determine fracture development by controlling the variation of mineral composition, rock structure, bed thickness, etc. Stress concentration is commonly high at fault tips, intersections, and overlaps, where fracture density is high and has good connectivity. The existence of abnormally high pressure reduces effective stress, promoting shear fracture development. Tensile overpressure fractures can also be generated under small levels of differential stress.
Supercritical Fluid Microcellular Foaming of High-Hardness TPU via a Pressure-Quenching Process: Restricted Foam Expansion Controlled by Matrix Modulus and Thermal Degradation
Authors: Chen Bichi , Jiang Junjie , Li Yaozong , Zhou Mengnan , Wang Zelin , Wang Liang , Zhai Wentao
Published: December 2022

High-hardness thermoplastic polyurethane (HD-TPU) presents a high matrix modulus, low-temperature durability, and remarkable abrasion resistance, and has been used in many advanced applications. However, the fabrication of microcellular HD-TPU foam is rarely reported in the literature. In this study, the foaming behavior of HD-TPU with a hardness of 75D was investigated via a pressure-quenching foaming process using CO2 as a blowing agent. Microcellular HD-TPU foam with a maximum expansion ratio of 3.9-fold, a cell size of 25.9 μm, and cell density of 7.8 × 108 cells/cm3 was prepared, where a high optimum foaming temperature of about 170 °C had to be applied with the aim of softening the polymer’s matrix modulus. However, the foaming behavior of HD-TPU deteriorated when the foaming temperature further increased to 180 °C, characterized by the presence of coalesced cells, microcracks, and a high foam density of 1.0 g/cm3 even though the crystal domains still existed within the matrix. The cell morphology evolution of HD-TPU foam was investigated by adjusting the saturation time, and an obvious degradation occurred during the high-temperature saturation process. A cell growth mechanism of HD-TPU foams in degradation environments was proposed to explain this phenomenon based on the gas escape through the defective matrix.
Optical Rotation—A Reliable Parameter for Authentication of Honey?
Authors: Gerginova Dessislava , Kurteva Vanya , Simova Svetlana
Published: December 2022

The controversial question of whether optical rotation data can be used to distinguish floral from honeydew honey was investigated. Specific optical rotation angles were determined for 41 honey samples, including floral, honeydew, and adulterated honey, indicating that moderate to high positive optical rotation angles were found for all adulterated samples measured. A strong correlation between the sugar profile and the specific optical rotation angle of honey was confirmed, and a method based on 13C NMR metabolomics was proposed to calculate specific optical rotation angles with good correlation with the experimental values. The results indicate that optical rotation is not a reliable method for distinguishing the origin of honey but could indicate adulteration.
Effect of Bear Garlic Addition on the Chemical Composition, Microbiological Quality, Antioxidant Capacity, and Degree of Proteolysis in Soft Rennet Cheeses Produced from Milk of Polish Red and Polish Holstein-Friesian Cows
Authors: Najgebauer-Lejko Dorota , Pluta-Kubica Agnieszka , Domagała Jacek , Turek Katarzyna , Duda Iwona , Golian Jozef
Published: December 2022

This study aimed to assess the effect of milk source and bear garlic addition on the selected properties of soft rennet cheese. Cheeses were produced from cow milk derived from two sources: Polish Red cows (PR) and Polish Holstein-Friesian cows (PHF) with a 0.5% (w/w) addition of bear garlic (Allium ursinum L.) dried leaves. Chemical composition and fatty acid profiles (GC) were determined in fresh cheeses. Fresh and stored for two weeks cheeses were subjected to microbiological studies, i.e., total aerobic bacteria count (TABC); count of Lactococcus sp., yeast and molds; coliforms; analysis of the proteolysis extension by means of o-phthaldialdehyde (OPA) assay and free amino acids content (HPLC); antioxidant capacity as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing antioxidant power (FRAP); as well as pH and water activity. Cheeses with bear garlic herbs were more prone to proteolysis but this was not accompanied by any effect on the microbial counts, water activity or pH. Cheeses produced from PR milk contained less monounsaturated fatty acids (MUFA) but were richer in n-3 PUFA and had a lower n-6/n-3 FA ratio than cheeses from PHF milk. Bear garlic addition increased DPPH anti-radical power but had less of an effect on the FRAP values.
Monitoring tert-Butylhydroquinone Content and Its Effect on a Biolubricant during Oxidation
Authors: Nogales-Delgado Sergio , Cabanillas Agustina Guiberteau , Romero Ángela García , Encinar Martín José María
Published: December 2022

The use of biolubricants as a replacement for petroleum-based products is becoming more and more important, due to the current global energy and crude oil scenario. Thus, the production of biolubricants (which could take place in biorefineries) should be as efficient as possible, obtaining high-quality products with suitable viscosity or oxidation stability values to compete with oil refineries. One of the ways to produce biolubricants is through double transesterification from vegetable oils, where the role of catalysts (usually homogeneous) is vital, as they can improve the yield of the process. However, they should be removed after the chemical reaction, which is difficult once the biolubricant is obtained. Otherwise, they could act as catalysts during oxidation, contributing to a further decrease in oxidation stability and provoking significant changes. To avoid this, antioxidant addition could be an interesting choice. The aim of this work was to assess TBHQ addition in frying oil biolubricants, monitoring properties such as viscosity, acid number, absorbance or TBHQ content (through voltammetry) during oxidation. TBHQ addition (2114 mg·L−1) kept the main quality parameters during oxidation compared to control samples. In contrast, TBHQ content decreased during oxidation (to 160 mg·L−1), which proved its antioxidant effect.
Nutritional Attributes and Phenolic Composition of Flower and Bud of Sophora japonica L. and Robinia pseudoacacia L.
Authors: Tian Jing , Gong Yuhong , Li Jun
Published: December 2022

Sophora japonica L. (SJL) and Robinia pseudoacacia L. (RPL) are widely cultivated in China. However, the utilization of their main by-products are limited due to a lack of comprehensive nutritional attributes. Herein, the proximate composition, mineral elements, fatty acids, amino acids, monosaccharides, and phenolics were analyzed to investigate the nutritional attributes of SJL and RPL. Dietary fiber was the main ingredient in SJL and RPL, followed by protein and lipids. The content of Fe in SJL and RPL was highest, especially in flowers of SJL, reaching about 1179.51 mg/kg. The total unsaturated fatty acids accounted for 89.67% of the bud of SJL. Meanwhile, the essential amino acids contents of the flower and bud of SJL and RPL accounted for 35.95–40.59% of total amino acids. The flower of SJL (373.75 mg/g) exhibited the most abundant monosaccharides. Meanwhile, the total phenolics and flavonoid contents in the buds of SJL and RPL were significantly higher than that of the flower, implying the buds possessed better biological activity. Moreover, the bud of SJL possessed the most abundant phenolics. The results provided a reference for the development of functional food derived from SJL and RPL.
Can We Form Mesoporous Zeolites by Steam Assisted Crystallization of MCM-41?
Authors: Souza Iane , Pergher Sibele , Sachse Alexander
Published: December 2022

The possibility of crystallizing silicalite-1 (MFI) from the pore walls of as-synthesized MCM-41 via steam-assisted crystallization (SAC) was thoroughly investigated. A kinetic study was conducted through the impregnation of as-synthesized MCM-41 with the structure-directing agent tetrapropyl-ammonium hydroxide (TPAOH). Materials obtained after different SAC treatment times (1–288 h) were characterized by XRD, nitrogen physisorption at 77 K, TGA/DTA, and SEM. The achieved results allowed us to conclude that during SAC treatment, rapid destruction of the hexagonal mesophase occurs with the enlargement of mesopores, probably by their coalescence, until achieving non-porous amorphous silica. Only thereafter is the crystallization of the MFI phase evidenced through the development of micron-sized (>10 µm) MFI structured crystals. This study suggests the probable practical impossibility of even partial crystallization of the pore walls of mesoporous materials by SAC.
In Vitro Assessment on Designing Novel Antibiofilms of Pseudomonas aeruginosa Using a Computational Approach
Authors: Rachmawati Dian , Fahmi Mochammad Zakki , Abdjan Muhammad Ikhlas , Wasito Eddy Bagus , Siswanto Imam , Mazlan Nurzafirah , Rohmah Jazirotur , Baktir Afaf
Published: December 2022

An anti-biofilm that can inhibit the matrix of biofilm formation is necessary to prevent recurrent and chronic Pseudomonas aeruginosa infection. This study aimed to design compounds with a new mechanism through competitive inhibitory activity against phosphomannomutase/phosphoglucomutase (PMM/PGM), using in vitro assessment and a computational (in silico) approach. The active site of PMM/PGM was assessed through molecular redocking using L-tartaric acid as the native ligand and other small molecules, such as glucaric acid, D-sorbitol, and ascorbic acid. The docking program set the small molecules to the active site, showing a stable complex formation. Analysis of structural similarity, bioavailability, absorption, distribution, metabolism, excretion, and toxicity properties proved the potential application of ligands as an anti-biofilm. In vitro assessment with crystal violet showed that the ligands could reach up to 95.87% inhibition at different concentrations. The nitrocellulose membrane and scanning electron microscopic visualization showed that the untreated P. aeruginosa biofilm was denser than the ligand-treated biofilm.
Removal of Cs-137 Radionuclide by Resorcinol–Formaldehyde Ion-Exchange Resins from Solutions Simulating Real Liquid Radioactive Waste
Authors: Tokar Eduard , Tutov Mikhail , Bratskaya Svetlana , Egorin Andrei
Published: December 2022

The efficiency of the removal of Cs-137 radionuclides with porous and non-porous resorcinol–formaldehyde resins from alkaline solutions simulating the composition of real liquid radioactive waste (LRW) streams has been evaluated. Resins were synthesized through the polycondensation of resorcinol and formaldehyde in an alkaline medium at a molar ratio of 1.8/2.2 and a temperature of 210 °C. The Cs-137 distribution coefficients on RFRs in alkaline solutions simulating LRW were above 103 mL/g under static sorption conditions. In a model solution with pH 11, the full dynamic sorption capacity of non-porous RFR was 0.178 mmol/g. The values of the full dynamic sorption capacities of porous RFRs were 0.274 and 1.035 mmol/g for resins obtained with calcium carbonate and toluene as templates, respectively. When the sizes of RFR beads increased two-fold, the volume until 5% cesium breakthrough decreased by 20–40%. The most pronounced beneficial effect of the RFR’s porosity was observed at flow rates from 25 to 50 BV/h. It was shown that the negative effect of metal cations on Cs-137 uptake increases in the following order: Na+ < Mg2+ < Ca2+ < K+. The number of bed volumes of LRW-simulating solution decontaminated with RFRs until 5% cesium breakthrough was above 450; that is higher than the value of known commercially available analogs. The latter shows that the developed RFRs are promising for application in technological schemes of alkaline LRW processing.