bluuebunny/crossref_metadata_2025 huggingface dataset:
APLIKASI AKADEMIK SISWA BERBASIS SMS GATEWAY DI SMA NEGERI 1 ENAM LINGKUNG
Authors: Hasibuan Rajimar Suhal Hasibuan , Andrew Kurniawan Vadreas
Published: November 2022

Sebagai salah satu sekolah berstandar nasional yang menuju internasional, SMAN 1 ENAM LINGKUNG harus bisa meningkatkan kualitas pendidikannya untuk menjadi lebih baik, salah satunya dengan mengatasi masalah siswa yang tidak disiplin seperti siswa yang suka membolos (dari rumah berangkat sekolah tetapi tidak sampai di sekolah), cabut (pulang sebelum jam sekolah berakhir), dan jika diberikan surat pemanggilan orangtua sering kali siswa tidak menyampaikan kepada orangtuanya bahkan ada diantara siswa yang memberikan surat tersebut kepada orang yang disewanya untuk mewakili orangtuanya. Pembuatan aplikasi ini dilakukan atas dasar analisa dan pengamatan yang dilakukan pada SMAN 1 Enam Lingkung dengan terfokus pada masalah absensi atau kehadiran siswa. Tujuan dari pembuatan aplikasi ini agar dapat menghubungkan atau memfasilitasi antara pihak sekolah dengan pihak orangtua dalam berbagi informasi, sehingga orangtua dapat mengetahui perkembangan studi anaknya di sekolah. Aplikasi akademik berbasis SMS Gateway ini dirancang menggunakan bahasa pemograman PHP (Hypertext Preprocessor) dengan menggunakan metode perancangan SDLC(System Development Life Cycle) model pendekatan Waterfall. Langkah-langkah yang dilakukan dalam penelitian ini adalah analisis sistem dan perencanaan, analisis kebutuhan, desain sistem, implementasi, integrasi dan pengujian.
Bioeconomía y transiciones hacia la sostenibilidad
Authors: Flórez-Zapata Nathalia M.V. , Murcia López Mario Andrés , Arce Castellanos Leidy Paola
Published: November 2022

Aunque la riqueza mundial ha aumentado considerablemente en los últimos años, también se han hecho más dramáticos algunos desafíos ambientales que amenazan el bienestar humano, como el cambio climático y la pérdida de la biodiversidad. Como si fuera poco, la pandemia de la COVID19 ha generado una crisis social y económica sin precedentes, que además de estar interrelacionada con los desafíos ambientales arriba mencionados, ha agudizado problemas sociales como la pobreza y la desigualdad. Estas crisis nos obligan a replantear el enfoque y comprensión del desarrollo, de manera que se plantee una trayectoria de desarrollo más verde, resiliente e inclusiva (Banco Mundial, 2021).
Requerimientos del territorio a nivel nacional: avances conceptuales y metodológicos
Authors: Corzo Germán , Corral-Gómez Nicolás , Vargas Sergio
Published: November 2022

Abordar la planificación del territorio desde los requerimientos del sistema socioecológico pretende ampliar la capacidad de escucha de la sociedad a las señales que se generan desde el territorio, entendido este como la matriz natural, social y cultural. En tal sentido proponemos un marco conceptual de traducción de las señales, a partir de información oficial y aquella generada a través de investigación científica de los institutos del Sistema Nacional Ambiental (SINA). Se incluyeron análisis tales como el desarrollado para el manual de compensaciones del medio biótico, los modelos de deforestación, el análisis de conectividad de áreas protegidas, los modelos de cambio en el régimen de precipitaciones, asociados a las tendencias de concentración de gases de efecto invernadero, entre otros. En los siguientes acápites se desarrollan los mecanismos de traducción de los indicadores, entendidos también como soluciones basadas en la naturaleza. Estos indicadores, aunque fueron construidos desde una base espacial y temporal definida (escala 1:100 000 y coberturas de la tierra entre 2012 y 2015) a partir del “Mapa de ecosistemas continentales de Colombia” (Ideam et al., 2017), pueden ser usados en contextos específicos, mediante aplicaciones en construcción, para su actualización y ajuste tanto espacial como temporal.
Meteorological influences of a major dust storm in Southwest Asia during July–August 2018
Authors: Alter Ross , LeGrand Sandra , Spates Freddie , Ledbetter William , Minnigan Sherman , Thompson John , Carter Kindra , Elliott Phillip
Published: November 2022

Dust storms can be hazardous for aviation, military activities, and respiratory health and can occur on a wide variety of spatiotemporal scales with little to no warning. To properly forecast these storms, a comprehensive understanding of the meteorological dynamics that control their evolution is a prerequisite. To that end, we chose a major dust storm that occurred in Southwest Asia during July–August 2018 and conducted an observation-based analysis of the meteorological conditions that influenced the storm’s evolution. We found that the main impetus behind the dust storm was a large-scale meteorological system (i.e., a cyclone) that affected Southwest Asia. It seems that cascading effects from this system produced a smaller, near-surface warm anomaly in Mesopotamia that may have triggered the dust storm, guided its trajectory over the Arabian Peninsula, and potentially catalyzed the development of a small low-pressure system over the southeastern end of the peninsula. This low-pressure system may have contributed to some convective activity over the same region. This type of analysis may provide important information about large-scale meteorological forcings for not only this particular dust storm but also for future dust storms in Southwest Asia and other regions of the world.
Left barrier loss for unbiased survival analysis prediction
Authors: shtossel oshrit , Dubovski Bar , Koren Omry , Louzoun Yoram
Published: November 2022

Survival analysis (SA) prediction involves the prediction of the time until an event of interest occurs (TTE), based on input attributes. The main challenge of SA is instances where the event is not observed (censored). Censoring can represent an alternative event (e.g., death) or missing data.

Most SA prediction methods suffer from multiple drawbacks that limit the usage of advanced machine learning methods: A) Simplistic models, B) Ignoring the input of the censored samples, C) No separation between the model and the loss function, and D) Typical small datasets and high input dimensions.

We propose a loss function, denoted suRvival Analysis lefT barrIer lOss (RATIO), that explicitly incorporates the censored samples input in the prediction, but still accounts for the difference between censored and uncensored samples. RATIO can be incorporated with any prediction model. We further propose a new data augmentation (DA) method based on the TTE of uncensored samples and the input of censored samples.

We show that RATIO drastically improves the precision and reduces the bias of SA prediction, and the DA allows for the inclusion of high-dimension data in SA methods even with a small number of uncensored samples.
A study on occupational stress, mental health and impact on work capacity of biosafety laboratory workers in China
Authors: Ju KeKe , abdurezhake Yakup , Wu Ruikai , Chen Yuming , Lu Yaoqin
Published: November 2022

Background
Biosafety laboratory (BSL) is engaged in highly pathogenic microbial experimental activities, because the operation of pathogenic microorganisms are highly infectious, pathogenic and no means of prevention and treatment, etc., often make the staff in the experimental activities under greater psychological pressure, in a state of stress and stress, may lead to a decline in work capacity or failure
Methods
An electronic questionnaire was used to survey 1162 BSL staff in Xinjiang as the study population. The Self-Rating Anxiety Scale (SAS), the Effort-Reward imbalance (ERI) scale, and the Work Capacity Index Questionnaire were used to measure their anxiety, occupational stress, and work capacity levels. The Mann-Whitney U test or Kruskal-Wallis H test was used to compare the occupational Stress, Anxiety, and Work Ability scores. The effects of demographic and work-related variables, i.e., confounding factors, were eliminated to the maximum extent possible by the Propensity Matching Scores (PSM) method. Multifactorial analysis of the relationship between anxiety and occupational strain and work ability of BSL employees. The relationship between occupational stress, anxiety, and work ability was quantified by building structural equation models through AMOS 26.0 software.
Results
The median and 25th and 75th percentile scores of anxieties, occupational stress, and work ability of the study participants were 48.75 (41.25, 57.50), 6.0 (5.0, 8.0), and 39.0 (36.0, 42.0) points, respectively. After propensity matching to eliminate confounders, multiple logistic regression analysis revealed that anxiety (OR = 1.08,95% CI:1.04 ~ 1.12), effort dimension (OR = 1.02,95% CI:0.93 ~ 1.13) and reward dimension (OR = 1.13,95% CI:1.06 ~ 1.21) significantly affected BSL employees' work ability (all p < 0.001). The results of structural equation modeling showed that both anxiety and occupational stress among BSL employees had direct effects on work ability with standardized path coefficients (β) of -0.42 and − 0.35, respectively (both P < 0.001), and occupational stress could indirectly affect work ability through anxiety (β=-0.24, P < 0.001).
Conclusion
Our findings suggest that anxiety and occupational strain are extremely prevalent among BSL employees. In addition, work ability decreases with increasing anxiety and occupational stress, and improvement of anxiety and occupational stress can improve the work ability of BSL employees.
Multi-parametric observations of intermittent hydrothermal discharge in West Crater of Iwo-Yama volcano, Kirishima Volcanic Complex, Japan
Authors: Tanabe Harutou , Matsushima Takeshi , Aizawa Koki , Muramatsu Dan
Published: November 2022

From April to July 2021, West Crater at Iwo-Yama, Kirishima Volcanic Complex, Japan, was repeatedly filled with hydrothermal water and subsequently evacuated. The overall cycle lasted 14–70 h, and the course of a single cycle followed this sequence of phases: (i) steam effusion disappeared 20–40 min before hydrothermal discharge; (ii) hydrothermal discharge occurred, generating a hydrothermal water pool; (iii) steam effusion resumed and gradually increased; and (iv) drain-back (evacuation) of the hydrothermal water occurred 1–1.5 h before the onset of the next hydrothermal discharge. We used multi-parametric observations (optical camera, thermometer, electric self-potential (SP), seismometer, acoustic sensor, and tiltmeter) to investigate the cause of the cyclic hydrothermal discharge. A change in SP data occurred approximately 2 h before the onset of hydrothermal discharge. However, the change in SP was small when hydrothermal discharge did not occur. The temporal change in SP is inferred to have been caused by groundwater flow through the region below West Crater, implying that groundwater flow was occurring 2 h before hydrothermal discharge. The polarity of SP change suggests that groundwater flowed toward the region underlying the vents. Seismic signals in the frequency range of < 20 Hz decreased 15–45 min after the onset of change in SP. This seismic signal pattern is inferred to have been caused by bubble activity in boiling fluid. We interpret that the inflow of cold groundwater inhibited boiling activity in the conduit, which in turn caused the cessation of both steam effusion and seismic activity. SP data suggest that the inflow of cold groundwater gradually decreased before hydrothermal discharge. Pressurization sufficient to force the water in the upper part of the conduit to ascend could have built up in the lower part of the conduit owing to a decrease in the input of groundwater into the upper part of the conduit and the continuing supply of steam bubbles and hot water. This increase in pressure finally led to hydrothermal discharge at the surface. We suggest that the inflow of cold groundwater into the geyser conduit was the key control on the occurrence and cyclicity of hydrothermal discharge in West Crater at Iwo-Yama.
NAFLD is associated with less severe liver fibrosis in chronic hepatitis B: a multi-center, retrospective study
Authors: Wang Jian , Lu Sufang , Xue Ruifei , Qiu Yuanwang , Chen Yuxin , Liu Jiacheng , Yao Renling , Zhu Li , Zhan Jie , Jiang Suling , Yin Shengxia , Tong Xin , Ding Weimao , Li Jie , Zhu Chuanwu , Huang Rui , Wu Chao
Published: November 2022

Background and objective: Chronic hepatitis B (CHB) is vulnerable to more severe liver diseases, and is often accompanied by non-alcoholic fatty liver disease (NAFLD). NAFLD shares risk factors with liver fibrosis and cirrhosis in CHB, but the influence of NAFLD on progression is controversial. This retrospective study evaluated the prevalence of NAFLD in patients with CHB and investigated associations between NAFLD and liver fibrosis in these patients, in a large multi-center liver biopsy cohort.
Methods: The cases of 1496 treatment-naïve patients with CHB who underwent liver biopsy were analyzed. Propensity score matching (PSM) was performed to adjust the confounders between patients with and without NAFLD.
Results: NAFLD was diagnosed via liver biopsy in 290 (19.4%) patients. Compared to the non-NAFLD group, patients with NAFLD showed lower rates of significant liver fibrosis, advanced liver fibrosis, and cirrhosis (P<0.001, =0.003, =0.013, respectively). After PSM adjusted for age, gender, hepatitis B surface antigen, and hepatitis B virus DNA, 273 patients remained in each group. Liver fibrosis remained significantly less severe in the NAFLD group, compared with the non-NAFLD. NAFLD was an independent negative factor of significant liver fibrosis or advanced liver fibrosis in patients with CHB.
Conclusions: CHB is often concomitant with NAFLD. The presence of NAFLD in CHB is associated with less severe liver fibrosis.
NDVI forecasting model based on the combination of Time series decomposition and CNN - LSTM
Authors: Gao Peiqiang , Du Wenfeng , Lei Qingwen , Li Juezhi , Zhang Shuaiji , Li Ning
Published: November 2022

Normalized difference vegetation index(NDVI) is the most commonly used factor to reflect vegetation growth status, and improving the prediction accuracy of NDVI is of great significance to the development of regional ecology. In this study, a new NDVI forecasting model based on the combination of time series decomposition(TSD), convolutional neural network (CNN) and long short-term memory (LSTM) was proposed. In order to verify the performance of TSD-CNN-LSTM model and explore the response of NDVI to climatic factors, two forecasting models of temperature and precipitation based on its own historical information and four NDVI forecasting models that based on Temperature, precipitation and its own historical information were established. The results show that TSD-CNN-LSTM model based on its own historical information had the best forecasting performance, with the RMSE, NSE, MAE to be 0.4996, 0.9981, 0.4169 for temperature, 5.6941, 0.9822, 3.9855 for precipitation and 0.0573, 0.9617, 0.0447 for NDVI, respectively. Meanwhile, the NDVI forecasting models based on climatic factors show that the model based on the combination of temperature and precipitation has the better effect than that based on single temperature and single precipitation. Combined with the results of correlation analysis it can be inferred that NDVI changes are most significantly influenced by the combination of temperature and precipitation, followed by temperature, and least influenced by precipitation. The above findings can provide a meaningful reference and guidance for the study of vegetation growth with climate changes.
Application of integrated equipment quality management mode of operating room and supply room in prevention and control of nosocomial infection
Authors: Zhang Baofeng , Peng Ding
Published: November 2022

Backgroud: This paper researches the influence of the integrated equipment quality management model of operating room and supply room and conventional equipment disinfection management scheme on the prevention and control of nosocomial infection.
Methods: 200 surgical instruments and surgical instrument kits in the supply room of our hospital from May 2020 to May 2022 are selected as research cases. They are equally divided into the experimental group and the control group by the random number table method, and each group is equipped with 100 pieces of surgical instruments and instrument kit. The control group adopts the conventional equipment disinfection management scheme, and the experimental group adopts the integrated equipment quality management model of operating room and supply room. The number of different types of surgical instruments and surgical instrument kits, the qualified rate of surgical instrument cleaning, the qualified rate of disinfection of surgical instrument kits, the wound infection rate of surgical patients, and the satisfaction of medical staff with the quality of the instruments are compared between the two groups.
Results: The results show that the different types of surgical instruments (lumen instruments, hemostatic forceps, and scissors) and the number of surgical instrument kits (puncture kits, accessories kits, and instrument kits) in the experimental group are not significantly different from those in the control group (P > 0.05). The qualified rate of disinfection of surgical instrument kits in the experimental group is significantly higher than that in the control group, and the difference is statistically significant (P < 0.05). The wound infection rate of surgical patients in the experimental group is significantly lower than that in the control group, and the difference is statistically significant (P< 0.05). The satisfaction rate of 50 medical staff with the quality of surgical instruments in the experimental group is significantly higher than that in the control group, and the difference is statistically significant (P < 0.05).
Conclusion: It is found that compared with the conventional equipment disinfection management scheme, the integrated equipment quality management model of operating room and supply room can significantly improve the cleaning and disinfection quality of surgical instruments and surgical instrument kits, reduce the risk of postoperative wound infection of surgical patients, improve the satisfaction of medical staff, and achieve the prevention and control of nosocomialinfection.
Genome-wide association analysis identifies quantitative trait loci for growth and fatness in Diannan small-ear (DSE) pigs
Authors: Liu Mei , Lan Qun , Yang Long , Deng Qiuchun , Wei Taiyun , Zhao Heng , Peng Peiya , Lin Xiaoding , Chen Yuhan , Ma Haiming , Wei Hongjiang , Yin Yulong
Published: November 2022

Background: In the livestock industry, the growth and fatness traits are directly related to production efficiency and economic profits. As for Diannan small-ear (DSE) pigs, a unique indigenous breed, the genetic architecture of growth and fatness traits are still elusive. The aim of this study was to search the genetic loci and candidate genes associated with phenotypic traits in DSE pigs using GWAS based on the Geneseek Porcine 50K SNP Chip data.
Results: A total of 30,648 single nucleotide polymorphisms (SNPs) were detected in 265 DSE pigs. After filtering, 22,146 SNPs were used for Genome-wide association studies (GWAS) analysis. The significant phenotypic correlations among eight important growth traits (body weight, back-fat thickness, and six body measurement traits) were observed. Seven SNPs surpassing the suggestively significant threshold for back height, backfat thickness, chest circumference and cannon bone circumference. Based on gene annotation results, these seven SNPs were mapped to the following candidate genes, VIPR2, SLC10A2, NUCKS1, MCT1, CHCHD3, SMOX and GPR1, which are mainly involved with adipocyte differentiation, lipid metabolism, skeletal muscle development, and average daily weight gain.
Conclusions: Our work offers novel insights into the genetic architecture of swine important economic traits and may play an important role in molecular markers breeding for DSE pig.
Feasibility of applying hyperspectral imaging as a rapid method for estimating potassium content across different plant species datasets
Authors: Baranowski Piotr , Siedliska Anna , Krzyszczak Jaromir , Banach Artur , Siłuch Marcin , Bartmiński Piotr , Wolińska Agnieszka , Tkaczyk Przemysław
Published: November 2022

Purpose The accurate and frequent estimation of the leaf plant potassium concentration enabled by hyperspectral imaging techniques has allowed growers to optimize fertilizer applications and reduce the negative impact on the environment. In this study, we examined the feasibility of using leaf spectral data to accurately estimate the potassium content in sugar beet and celery plants.
Methods Leaf images in the visible and near infrared region (VNIR: 400–1000 nm) and short-wavelength infrared region (SWIR: 1000-2500 nm) were captured by a hyperspectral camera. The potassium content was measured by ordinary destructive laboratory methods. The correlation-based feature selection (CFS) algorithm was implemented to select important wavelengths that carried the most useful information for predicting the potassium content in plant leaves. Four multivariate regression methods were tested to find a model with strong predictive performance.
Results The experimental results showed that the Random Forest (RF) model using 12 bands (425, 443, 479, 599, 631, 662, 798, 863, 897, 921, 1978 and 2053 nm) had the highest accuracy for predicting potassium content in sugar beet, celery and both plant datasets (Rp2 = 0.85, Rp2 = 0.79, and Rp2 = 0.81, respectively).
Conclusion The results confirm the universality of the described method. Although further validation studies involving other plant species are needed, it appears that the spectral reflectance technique could be a promising tool for the rapid, noninvasive and cost-effective estimation of K content in plant leaves, contributing to a significant step forward in precision fertilization management.
MechRetro is a chemical-mechanism-driven graph learning framework for interpretable retrosynthesis prediction and pathway planning
Authors: Wang Yu , Pang Chao , Wang Yuzhe , Jiang Yi , Jin Junru , Liang Sirui , Zou Quan , Wei Leyi
Published: November 2022

Leveraging artificial intelligence for automatic retrosynthesis speeds up organic pathway planning in digital laboratories. However, existing deep learning approaches are unexplainable, like "black box" with few insights, notably limiting their applications in real retrosynthesis scenarios. Here, we propose MechRetro, a chemical-mechanism-driven graph learning framework for interpretable retrosynthetic prediction and pathway planning, which learns several retrosynthetic actions to simulate a reverse reaction via elaborate self-adaptive joint learning. By integrating chemical knowledge as prior information, we design a novel Graph Transformer architecture to adaptively learn discriminative and chemically meaningful molecule representations, highlighting the strong capacity in molecule feature representation learning. We demonstrate that MechRetro outperforms the state-of-the-art approaches for retrosynthetic prediction with a large margin on large-scale benchmark datasets. Extending MechRetro to the multi-step retrosynthesis analysis, we identify efficient synthetic routes via an interpretable reasoning mechanism, leading to a better understanding in the realm of knowledgeable synthetic chemists. We also showcase that MechRetro discovers a novel pathway for protokylol, along with energy scores for uncertainty assessment, broadening the applicability for practical scenarios. Overall, we expect MechRetro to provide meaningful insights for high-throughput automated organic synthesis in drug discovery.
Leaf nutrient resorption and response to soil nutrient availability of different life-form urban garden trees
Authors: Hu Ruyuan , Liu Tairui , Zheng Rongrong , Zhang Yunxiang , Guo Jinping
Published: November 2022

Typical garden tree species are very representative in studying material cycle of urban ecosystem. To study the response of nutrient storage and resorption strategies of different life-form garden tree species to soil nutrients is the key to the sustainability of the urban garden ecosystem. In this research, 9 sample trees out of every 40 garden tree species, which were classified into 2 life forms, were selected for repeated sampling. Nitrogen (N) and phosphorus (P) concentrations in green and senesced leaves and soil nutrient concentrations were investigated, respectively. By comparing nutrient concentrations and resorption of different life-form tree species, the utilization strategies of soil nutrients by different life-form tree species were further analysed. N concentration was substantially higher in deciduous plants with green and senesced leaves than in evergreen ones. Green leaves of deciduous plants had much greater P concentrations than those of evergreen plants, although the difference between the two was not statistically significant. Leaf N:P between different life forms was less than 14, showing a tendency to N limitation. Nitrogen resorption efficiency (NRE) and phosphorous resorption efficiency (PRE) in deciduous plants were significantly higher than in evergreen plants. NRE in deciduous plants was significantly positively correlated with PRE, but not significant in evergreen plants. NuRE of evergreen plants increased as soil N and P concentrations increased, but NuRE of deciduous plants dropped as soil N and P concentrations increased. Compared with deciduous plants, evergreen plants were more sensitive to soil N and P concentrations. These findings have important implications for urban garden trees management practices in this region.
Multiple cystic germinomas presenting as focal dystonia of fingers: a case report
Authors: Han Seungju , Cho Hwa Jin , Lee Bo Lyun , Lee Keun Soo
Published: November 2022

Intracranial germinomas predominantly develop on or near the midline structure in children and young adults and are diagnosed by brain imaging and biopsy. However, if brain imaging and pathology show unusual findings, it becomes difficult to make an accurate diagnosis. Herein, wereport the case of a 14-year-old boy who presented with focal dystonia of the fingers as an initial symptom. Magnetic resonance imaging of the brain showed multifocal heterogeneous lesions with solid and cystic components involving the right frontal lobe, hypothalamus, corpus callosum, left basal ganglia, and left corona radiata. A stereotactic biopsy of the right frontal lesion revealed several granulomatous areas with abundant inflammatory cells. After immunohistochemical staining, the patient was diagnosed with germinoma and treated with chemoradiotherapy according to the Korean Society for Pediatric Neuro-Oncology protocol. The patient has been in complete remission for five years. Germinomas can develop in intracranial off-midline structures, with unusual clinical, radiological, and pathological presentations. It is important to include intracranial germinomas in the differential diagnosis of infiltrative parenchymal tumors, especially in children.
Autophagy limits inflammatory gene expression through targeting of nuclear p65/RelA by LC3 and p62 for lysosomal degradation
Authors: Brischetto Cristina , Mucka Patrick , Kaergel Eva , Scheidereit Claus
Published: November 2022

The interplay between NF-κB signaling and autophagy regulates inflammatory signaling in different cellular contexts and in response to different stimuli. The impairment of this crosstalk may play a role in chronic inflammation and in tumorigenesis. However, the molecular mechanism by which these two pathways interact to regulate the inflammatory response remains elusive. By using biochemical analysis and imaging techniques, we characterized the interaction of the endogenous autophagic marker LC3 and NF-κB/p65 in response to different stress conditions. Following irradiation or TNFα stimulation, nuclear accumulation of LC3 strongly co-localized with p65, suggesting that nuclear p65 is targeted for autophagic degradation. Mechanistically, we showed that the nuclear p65-LC3 interaction is mediated by ubiquitination of the same p65, which is recognized by the cargo receptor p62, resulting in its cytoplasmic export and lysosomal proteolysis. Accordingly, autophagy inhibition by depletion of the essential autophagy gene ATG16L1 selectively stabilizes nuclear p65, in turn enhancing NF-κB gene expression of pro-inflammatory cytokine. Our results revealed a novel molecular mechanism that modulates the NF-κB inflammatory response through nuclear sequestration of the NF-κB/p65 subunit by autophagy proteins. These findings are of importance for developing novel therapeutic strategies against chronic inflammatory diseases displaying defective autophagy and constitutive NF-κB activity.
Targeting cancer-associated fibroblast autophagy renders pancreatic cancer eradicable with immunochemotherapy by inhibiting adaptive immune resistance
Authors: Zhang Xiaozhen , Lao Mengyi , Yang Hanshen , Sun Kang , He Lihong , Li Muchun , Ying Honggang , Chen Yan , Bai Xueli , Liang Tingbo
Published: November 2022

Background
Accumulating evidence suggests that cancer-associated fibroblast (CAF) autophagy is crucial in tumor development and may be a therapeutic target for pancreatic ductal adenocarcinoma (PDAC). However, the role of CAFs autophagy during immune surveillance and cancer immunotherapy is unclear.
Methods
In vivo efficacy and mechanistic studies, using ATG5f/f α-SMAcreERT2 mice, KPC-genetically engineered mouse model (GEMM) mice, C57BL/6 mice and nude mice with KPC cell‑derived orthotopic tumors, employed immune checkpoint blockades (ICBs) and gemcitabine. Mass cytometry (CyTOF), immunohistochemistry (IHC), and flow cytometry analyzed local and systemic alterations in the immunophenotype. The regulatory effects of CAFs autophagy on tumour PD-L1 and its downstream signaling were analyzed by RNA-seq, 4D mass spectrometer (MS), IP-MS, western blotting, GST-pulldown, immunofluorescence (IF), IHC, and flow cytometry.
Results
The present study revealed that the inhibition of CAF autophagy suppresses in vivo tumor development in immune-deficient xenografts. This deletion compromises anti-tumor immunity and anti-tumor efficacy both in vitro and in vivo by upregulating PD-L1 levels in an immune-competent mouse model. A deletion in CAF autophagy reduced the production of interleukin 6, disrupting high desmoplastic TME and decreasing USP14 expression at the transcription level in pancreatic cancer cells. We further identify USP14 as the post-translational factor responsible for downregulating PD-L1 expression by removing K63 linked-ubiquitination at the K280 residue. Finally, chloroquine diphosphate-loaded mesenchymal stem cell (MSC)-liposome, by accurately targeting CAFs, inhibited CAF autophagy, improving the efficacy of immunochemotherapy to combat pancreatic cancer.
Conclusions
Targeting CAFs autophagy disrupt the desmoplastic and immunosuppressive TME to enhance the efficacy of immunochemotherapy by inhibiting adaptive immune resistance.
Easily missed diagnosis of testicular choriocarcinoma with a large retroperitoneal mass as the primary symptom: a case report
Authors: Zhong Bin , Zhang Tao , Dong Yi , Yin Wei , Zhang Jian-Xin , Jin Wei-Dong
Published: November 2022

Testicular choriocarcinoma is a relatively rare malignancy with a highly aggressive nature. Timely diagnosis and treatment can help prolong the survival of patients and even cure them. This case reports a 29-year-old male who presented to the clinic for a month with epigastric pain without complaints of discomfort in the reproductive system. On examination, only a massive mass of approximately 9*10 cm could be palpated in the upper abdomen. When asked about his previous history, the patient only described a history of a right inguinal hernia that had been repaired 12 years earlier without knowing that a testicular descending fixation had been performed at the same time (we only found out afterward by asking his parents). The admission diagnosis was considered the retroperitoneal tumor, which was found to have metastasized to the liver and lungs after the completion of relevant tests. We then performed a CT-guided puncture biopsy of the lung tissue. The biopsy pathology suggested only a tiny heterotypic cell mass, and metastatic cancer was considered. As the symptoms of tumor compression gradually worsened, we nevertheless performed surgical treatment (retroperitoneal tumor resection + partial duodenal resection + enteroanastomosis). The postoperative pathology was choriocarcinoma. It was at this point that we pursued the patient's medical history in detail and, at the same time, performed a testicular ultrasound which revealed a substantial occupation of the right testicle. The diagnosis of testicular choriocarcinoma was finally considered (without pathological confirmation). We wanted to start salvage chemotherapy as soon as possible after surgery. However, the patient's postoperative condition was poor, with rapid progression of hepatopulmonary metastases and gradually increased thyrotoxicosis, and we started salvage chemotherapy (EP regimen: etoposide and cisplatin) on postoperative day 12. However, the patient was forced to stop due to a severe chemotherapy reaction and eventually died of respiratory and cardiac arrest in hospital. For men presenting with a large retroperitoneal mass without reproductive system-related complaints, a detailed history of the inguinal testicle should still be inquired with a detailed physical examination. Meanwhile, laboratory and image tests should be performed to rule out the possibility of a genital tumor to avoid a missed diagnosis that could lead to delayed treatment.
Effect of long-term different land use on soil quality in an Entic Chromoxerert
Authors: Acar Mert , Çelik İsmail , Budak Mesut , Acir Nurullah
Published: November 2022

The differences in land use can potentially affect soil quality. In this respect, this study sought to assess the long-term effect of horticulture and arable lands on the soil quality of the clayey Arıklı soils (Entic Chromoxerert). Physical, chemical, biological and fertility indicators of the soil were analyzed to determine the soil quality index (SQI) using the Fuzzy Analytical Hierarchy Process (FAHP) and Geographical Information System (GIS) for a 225 ha area of the Arıklı soil series. The physical quality received the highest weight in the study. The highest weights in physical, chemical, biological and fertility quality were obtained in AWC, pH, OC and P indicators, respectively. SQI in arable lands ranged from 38.60 to 68.91%, with a mean of 51.77%. Compared to arable, SQI in horticulture varied within a narrower range and had a higher mean SQI (59.87%). In this study, 74.2% of the area had a low SQI and 25.8% had medium SQI. Conventional soil tillage and burning of stubble residues in the arable lands had a statistically negative effect on SQI. Compared to arable, in horticulture BD, OC, AWC, MBC, BGEA and K had better scores thus improving SQI. The results concluded that conventional soil tillage practice should be abandoned and reduced or no-till practices should be adopted.
An enriched environment improves maternal sleep deprivation- induced cognitive deficits via hippocampal histone acetylation
Authors: Zhang Yue-Ming , Wei Ru-Meng , Ni Ming-Zhu , Wu Qi-Tao , Li Yun , Ge Yi-Jun , Kong Xiao-Yi , Li Xue-Yan , Chen Gui-Hai
Published: November 2022

Growing evidence clearly demonstrates that maternal rodents exposure to sleep deprivation during late pregnancy impairs learning and memory in their offspring. Epigenetic mechanisms, particularly histone acetylation, are known to be involved in synaptic plasticity, learning, and memory. We investigated whether cognitive decline induced by sleep deprivation during late-pregnancy is associated with histone acetylation dysfunction and whether this effect could be reversed by an enriched environment (EE). In the present study, pregnant CD-1 mice were exposed to sleep deprivation during the third trimester of pregnancy. After weaning, all offspring were randomly assigned to two subgroups in either a standard environment or an EE. When offspring were three months old, the Morris water maze was used to evaluate hippocampal-dependent learning and memory ability. Molecular biological techniques, including western blot and real-time fluorescence quantitative polymerase chain reaction, were used to examine histone acetylation pathway and synaptic plasticity markers in the hippocampus of offspring. Our results showed that the following were all reversed by EE treatment: maternal sleep deprivation (MSD)-induced cognitive deficits including spatial learning and memory; histone acetylation dysfunction including increased histone deacetylase 2 (HDAC2) and decreased histone acetyltransferase (CBP), and the acetylation levels of H3K9 and H4K12; synaptic plasticity dysfunction including decreased brain-derived neurotrophic factor (BDNF) and postsynaptic density protein-95 (PSD-95). The results suggested that MSD could damage learning ability and memory in offspring via the histone acetylation pathway. This effect could be reversed by EE treatment.
Higher Risk of Car Accidents in Older Patients with Toxoplasma gondii in Yazd Province, Central Iran: A Cohort Study
Authors: Ghasemirad Hamidreza , Aalazade Maryam Sadat , Shariatpanahi Marjan , Owliaey Hamid , Kargar Meraj , Ghasemirad Mohammadreza , Chehrazad Mozhgan , Zare Mahdie
Published: November 2022

Background: Toxoplasmosis appears to be not only an infectious disease with observable symptoms but also one that can affect the social and behavioral aspects of both animals and humans. These social issues, particularly memory dysfunction, may result in dangerous occurrences such as traffic accidents. The purpose of this study was to investigate and compare the rate of traffic accidents among individuals with positive and negative Toxoplasma gondii (T. gondii) serology.
Materials and Methods: This was a retrospective cohort study conducted in the province of Yazd, central Iran, from 2018 to 2021 based on the statistics available in the province’s laboratories. We analyzed the results of the IgG test against Toxoplasma gondii collected in the laboratories of Yazd, Iran, from a total of 360 individuals with valid driver’s licenses who were divided into two groups. One group was Toxoplasma gondii-seropositive, while the other was negative. The traffic police system recorded the statistical analysis of traffic accidents from 2018 to 2021.
Results: The age of the toxoplasmosis group was slightly greater than that of the control group (36.2 and 34.08, respectively; p-value = 0.03). There was no significant difference between the toxoplasmosis group and the control group regarding driving time (p-value = 0.06). The incidence of accidents was greater in the toxoplasmosis group (36.7% vs. 20.6%; p < 0.001). This rate was significantly higher in people aged 35-69 than younger people (40% vs. 32.9%, respectively; p-value = 0.01).
Conclusion: Individuals with latent toxoplasmosis had a significantly higher risk of car accidents than those with negative serology, with the risk increasing with age. Due to contradictory findings regarding the effect of toxoplasmosis on traffic accidents and the higher incidence of toxoplasmosis in Persian cats, these findings should be considered when developing public health strategies.
A Case for a Binary Black Hole System Revealed via Quasi-Periodic Outflows
Authors: Pasham Dheeraj , Tombesi Francesco , Sukova Petra , Zajaček Michal , Rakshit Suvendu , Coughlin Eric , Kosec Peter , Karas Vladimir , Masterson Megan , Mummery Andrew , Holoien Thomas , Guolo Muryel , Hinkle Jason , Ripperda Bart , Witzany Vojtěch , Shappee Ben , Kara Erin , Horesh Assaf , Velzen Sjoert van , Sfaradi Itai , Kaplan David , Burger Noam , Murphy Tara , Remillard Ronald , Steiner James , Wevers Thomas , Arcodia Riccardo , Buchner Johannes , Merloni Andrea , Malyali Adam , Fabian Andrew , Fausnaugh Michael , Daylan Tansu , Altamirano Diego , Payne Anna , Ferrara Elizabeth , Burke Jamison
Published: November 2022

Binary black hole systems in close orbits are thought to be the precursors of gravitational wave events and their identification has fundamental implications for our understanding of black hole growth and evolution. However, a gravitationally bound companion orbiting closer than about a milli-parsec to an accreting supermassive black hole (SMBH, mass ≥106 solar masses) in an external galaxy cannot be spatially resolved with present-day instruments. Instead, its detection requires a combination of indirect observational methods and advanced modelling. Here we report the detection of quasi-periodic variability in X-ray spectral features after an outburst from a previously low-luminosity Active Galactic Nucleus (AGN) in a galaxy at redshift z=0.056. We interpret this variability as due to Quasi-Periodic Outflows (QPOuts) from the SMBH. The puzzling phenomenon repeats roughly once every 8.5 days and photo-ionization modeling of the energy spectra implies that the outflowing material moves with a high velocity of about a third of the speed of light. The mass of the central black hole, roughly 30 million solar masses, as derived from the optical broad line--SMBH mass scaling relation, implies that the outflows are launched from a few tens of gravitational radii from the SMBH event horizon. By performing 2D and 3D general relativistic magnetohydrodynamic (GRMHD) simulations and by comparing their results over a broad range of the system parameters we show that these QPOuts can be explained with an orbiting intermediate-mass black hole (IMBH) secondary (mass range of 100--10,000 solar masses) at a distance of about 100 gravitational radii (less than a milli-parsec) from the primary SMBH. Outflows are naturally enhanced when the secondary object punches through the SMBH's inner accretion disc during each orbital period and this is revealed as regular increases in X-ray absorption along our line of sight. The overall optical, UV and X-ray outburst properties indicate that a major accretion episode -- perhaps from a stellar tidal disruption by the SMBH -- could have provided the gas to illuminate the presence of the pre-existing IMBH secondary. Our work highlights the new astrophysical phenomenon of QPOuts and the importance of high cadence X-ray monitoring observations to potentially uncover electromagnetic signatures of such tight binary black hole systems.
The mediating effect of coping style in the relationship between sleep status and quality of life among night shift nurses: a multistage stratified cluster sampling survey
Authors: Zhao Yan , Yang Bei , Liu Cui , Lin Benmiao , Wu Xiaoyi , Sun Yuanyuan , Chu Jianying
Published: November 2022

Background:
This study explored how coping styles mediate the relationship between sleep status and quality of life among night shift nurses.
Methods:
A multistage stratified cluster sampling survey was conducted among staff at a general hospital from November 2019 to January 2020. A total of 1,170 night shift nurses completed the Pittsburgh Sleep Quality Index (PSQI), EuroQol five-dimensions (EQ-5D), and Simplified Coping Style Questionnaire (SCSQ). Respectively, these measures were used to assess sleep quality, the quality of life, and coping styles.
Results:
The total sleep status was positively correlated with the quality of life (r = 0.31, P < 0.05) and negative coping (r = 0.18, P < 0.05), negatively correlated with positive coping (r = -0.05, P < 0.05). Negative coping was positively correlated with the quality of life (r = 0.42, P < 0.05). According to the path analysis, sleep status had a positive effect on negative coping (β = 0.18, P < 0.05) and quality of life (β = 0.11, P < 0.05), negative effect on positive coping (β = -0.05, P < 0.05); positive coping had a negative effect on quality of life (β = -0.03, P < 0.05), while negative coping had a positive effect on quality of life (β = 0.02, P < 0.05).
Conclusions:
Coping style partially mediated the relationship between sleep status and quality of life. This suggests that interventions targeting coping styles may benefit night shift nurses, as such an approach can ultimately enhance the quality of life by improving sleep.
A qualitative research on mental health and psychosocial issues impacting Syrian refugee women in Turkey
Authors: Çöl Meltem , Akın-Sarı Burçin , Mut Ayşe Nur Usturalı , Çelik Kezban , Aykut Nihal Bilgili , Dinç Leyla Ezgi , Koçak Cemal , Kobeissi Loulou , Say Lale , Akın Ayşe
Published: November 2022

Background: Due to the civil war since 2011, around 4 million Syrian refugees have migrated to Turkey from their home countries. It is well known that being a refugee, especially a female refugee, is considered to be one of the highest risk group in conflict areas. The psychological adaption challenges and extreme feelings of homesickness have resulted in mental health issues that are more prevalent among refugee women. This study aims to understand how context-specific factors influence Syrian refugee women’ psychosocial wellbeing and mental health through focus group discussions.
Methods: A total of 9 focus groups were conducted with Syrian women (4), Syrian men (3) and stakeholders (2). Qualitative assessment was used to examine the experiences related to the psychological health difficulties of Syrian women living in Turkey with temporary protection status. Thematic analysis was run to analyze the data by coding and identifying themes or patterns. In this study, their psychosocial problems, intention to seek professional help, access to existing services, challenges in access to, and expectations regarding these services were evaluated.
Results: Refugee women experience psychological and social difficulties just because they are women. Also, refugee women seem to be more vulnerable to psychological problems than men due to the effect of patriarchal ideology and the problems experienced before the forced migration.
Conclusions:Psychosocial well-being of Syrian refugee women is affected by the social, economic and political atmosphere, and it is important to develop policies that address the needs of these women.
WITHDRAWN: Preparation of resveratrol-loaded ethyl cellulose microspheres via foam- transfer and its application performances
Authors: Wang Yun , Gao Mengyuan , Pan Xiaolin , He Yanping P. , Liu Yuyang , Ji Mei , Si Tian , Sun Yanlin
Published: November 2022

Resveratrol (Res) has been widely used in personal-care and pharmaceutical products since it has anti-oxidant, anti-inflammatory, cardioprotective, and anti-cancer properties. In this work, it was loaded by using biodegradable ethyl cellulose (EC) as the carrier to prepare EC/Res microspheresvia a foam-transfer method. The effects of experimental conditions on the properties of EC/Res microspheres were studied, and then their application performances including the sustained release and the degradation property were investigated. The results showed that resveratrol was loaded to EC microspheres successfully, in which EC/Res microspheres were in highly porous structures. Especially, when the PVA concentration = 2.5 wt%, the stirring rate = 600 rpm, the oil/water (O/W) ratio = 1:2, the polymer concentration = 1:25, the heating rate = 0.33 ℃/min, and the viscosity of ethyl cellulose = 90~110 mpa.s, the yield of EC/Res microspheres was up to 80.70 wt% with an encapsulation ratio of 73.89%; EC/Res microspheres were than produced continuously with an even higher encapsulation rate of resveratrol, which was 90.63% with a yield of 85.58 wt%. The cumulative release of EC/Res microsphere was up to 91.97 wt% in the phosphate buffer solution(PBS) at pH = 5.0 under 45 ℃ for 120 hours. After EC/Res microspheres degraded in PBS (pH = 7.4) for 45 days, their hardness decreased from 12.01 ± 0.56 MPa to 4.02 ± 0.45 MPa. This work provided a practical solution for resveratrol’s application as functional additives in a variety of areas.