bluuebunny/crossref_metadata_2025 huggingface dataset:
Neutrophils and Neutrophil Extracellular Traps Drive Necroinflammation in COVID-19
Authors: Tomar Bhawna , Anders Hans-Joachim , Desai Jyaysi , Mulay Shrikant R.
Published: June 2020

The COVID-19 pandemic is progressing worldwide with an alarming death toll. There is an urgent need for novel therapeutic strategies to combat potentially fatal complications. Distinctive clinical features of severe COVID-19 include acute respiratory distress syndrome, neutrophilia, and cytokine storm, along with severe inflammatory response syndrome or sepsis. Here, we propose the putative role of enhanced neutrophil infiltration and the release of neutrophil extracellular traps, complement activation and vascular thrombosis during necroinflammation in COVID-19. Furthermore, we discuss how neutrophilic inflammation contributes to the higher mortality of COVID-19 in patients with underlying co-morbidities such as diabetes and cardiovascular diseases. This perspective highlights neutrophils as a putative target for the immunopathologic complications of severely ill COVID-19 patients. Development of the novel therapeutic strategies targeting neutrophils may help reduce the overall disease fatality rate of COVID-19.
Experimental Models of Sarcopenia: Bridging Molecular Mechanism and Therapeutic Strategy
Authors: Mankhong Sakulrat , Kim Sujin , Moon Sohee , Kwak Hyo-Bum , Park Dong-Ho , Kang Ju-Hee
Published: June 2020

Sarcopenia has been defined as a progressive decline of skeletal muscle mass, strength, and functions in elderly people. It is accompanied by physical frailty, functional disability, falls, hospitalization, and mortality, and is becoming a major geriatric disorder owing to the increasing life expectancy and growing older population worldwide. Experimental models are critical to understand the pathophysiology of sarcopenia and develop therapeutic strategies. Although its etiologies remain to be further elucidated, several mechanisms of sarcopenia have been identified, including cellular senescence, proteostasis imbalance, oxidative stress, and “inflammaging.” In this article, we address three main aspects. First, we describe the fundamental aging mechanisms. Next, we discuss both in vitro and in vivo experimental models based on molecular mechanisms that have the potential to elucidate the biochemical processes integral to sarcopenia. The use of appropriate models to reflect sarcopenia and/or its underlying pathways will enable researchers to understand sarcopenia and develop novel therapeutic strategies for sarcopenia. Lastly, we discuss the possible molecular targets and the current status of drug candidates for sarcopenia treatment. In conclusion, the development of experimental models for sarcopenia is essential to discover molecular targets that are valuable as biochemical biomarkers and/or therapeutic targets for sarcopenia.
Solvent Effect on the Regulation of Urea Hydrolysis Reactions by Copper Complexes
Authors: Castro Caio B. , Silveira Rafael G. , Colombari Felippe M. , de Moura André Farias , Nascimento Otaciro R. , Marques Netto Caterina G. C.
Published: June 2020

Abiotic allosterism is most commonly observed in hetero-bimetallic supramolecular complexes and less frequently in homo-bimetallic complexes. The use of hemilabile ligands with high synthetic complexity enables the catalytic center by the addition or removal of allosteric effectors and simplicity is unusually seen in these systems. Here we describe a simpler approach to achieve kinetic regulation by the use of dimeric Schiff base copper complexes connected by a chlorido ligand bridge. The chlorido ligand acts as a weak link between monomers, generating homo-bimetallic self-aggregating supramolecular complexes that generate monomeric species in different reaction rates depending on the solvent and on the radical moiety of the ligand. The ligand exchange was observed by electron paramagnetic resonance (EPR) and conductivity measurements, indicating that complexes with ligands bearing methoxyl (CuIIL2) and ethoxyl (CuIIL5) radicals were more prone to form dimeric complexes in comparison to ligands bearing hydrogen (CuIIL1), methyl (CuIIL3), or t-butyl (CuIIL4) radicals. The equilibrium between dimer and monomer afforded different reactivities of the complexes in acetonitrile/water and methanol/water mixtures toward urea hydrolysis as a model reaction. It was evident that the dimeric species were inactive and that by increasing the water concentration in the reaction medium, the dimeric structures dissociated to form the active monomeric structures. This behavior was more pronounced when methanol/water mixtures were employed due to a slower displacement of the chlorido bridge in this medium than in the acetonitrile/water mixtures, enabling the reaction kinetics to be evaluated. This effect was attributed to the preferential solvation shell by the organic solvents and in essence, an upregulation behavior was observed due to the intrinsic nature of the complexes to form dimeric structures in solution that could be dismantled in the presence of water, indicating their possible use as water-sensors in organic solvents.
Effects of Cathode Voltage Pulse Width in High Power Impulse Magnetron Sputtering on the Deposited Chromium Thin Films
Authors: Kuo Chin-Chiuan , Lin Chun-Hui , Lin Yu-Tse , Chang Jing-Tang
Published: June 2020

Environmentally-safe high-power impulse magnetron sputtering (HiPIMS) technology was utilized to deposit chromium films. This research focused on the influences of the HiPIMS pulse widths on the microstructure of films deposited at different deposition pressures and substrate bias voltages. Under the conditions of the same average HiPIMS power and duty cycle, the deposition rate of the Cr thin film at working pressure 0.8 Pa is slightly higher than at 1.2 Pa. Also, the difference between deposition rates under two pressures decreases with the discharge pulse width. The deposition rate of the short pulse width 60 μs is lowest, but those of 200 and 360 μs are approximately the same. With no or small direct current substrate biasing, the microstructure of films coated at short pulse width is similar to the typical magnetron sputtering deposited films. Elongating the pulse width enhances the ion flux toward the substrate and changes the film structure from individual prism-like columns into tangled 3-point/4-point star columns. Substantial synchronized substrate biasing and longer pulse width changes the preferred orientation of Cr films from Cr (110) to Cr (200) and Cr (211). The films deposited at longer pulse width exhibit a higher hardness due to the reducing of intercolumn voids.
Estimation of Dynamic Bivariate Correlation Using a Weighted Graph Algorithm
Authors: John Majnu , Wu Yihren , Narayan Manjari , John Aparna , Ikuta Toshikazu , Ferbinteanu Janina
Published: June 2020

Dynamic correlation is the correlation between two time series across time. Two approaches that currently exist in neuroscience literature for dynamic correlation estimation are the sliding window method and dynamic conditional correlation. In this paper, we first show the limitations of these two methods especially in the presence of extreme values. We present an alternate approach for dynamic correlation estimation based on a weighted graph and show using simulations and real data analyses the advantages of the new approach over the existing ones. We also provide some theoretical justifications and present a framework for quantifying uncertainty and testing hypotheses.
A Gaussian-Distributed Quantum Random Number Generator Using Vacuum Shot Noise
Authors: Huang Min , Chen Ziyang , Zhang Yichen , Guo Hong
Published: June 2020

Among all the methods of extracting randomness, quantum random number generators are promising for their genuine randomness. However, existing quantum random number generator schemes aim at generating sequences with a uniform distribution, which may not meet the requirements of specific applications such as a continuous-variable quantum key distribution system. In this paper, we demonstrate a practical quantum random number generation scheme directly generating Gaussian distributed random sequences based on measuring vacuum shot noise. Particularly, the impact of the sampling device in the practical system is analyzed. Furthermore, a related post-processing method, which maintains the fine distribution and autocorrelation properties of raw data, is exploited to extend the precision of generated Gaussian distributed random numbers to over 20 bits, making the sequences possible to be utilized by the following system with requiring high precision numbers. Finally, the results of normality and randomness tests prove that the generated sequences satisfy Gaussian distribution and can pass the randomness testing well.
A Sustainable Solution for Energy Efficiency in Italian Climatic Contexts
Authors: Annibaldi Valeria , Cucchiella Federica , Rotilio Marianna
Published: June 2020

In the European Union, about 40% of energy consumption and 36% of CO2 emissions come from buildings; therefore, the improvement of their energy performance is a strongly focused issue. In particular, the energy efficiency of the building envelope is a very important element to pay attention to. Many studies have been conducted on this field of research, and the study illustrated in this paper also belongs to this topic. In particular, this article presents a multidisciplinary method to find sustainable solutions for energy efficiency in Italian climatic contexts using the Life Cycle Cost Analysis approach. In detail, this paper defines the reference scenario and then deepens the methodology used to determine the economically optimal thickness of a specific insulating material—hemp fiber—applied to a specific type of wall—uninsulated cavity walls made of hollow bricks, which are very widespread in Italy. The analysis is developed in relation to three different regions—Piedmont, Abruzzo, and Campania. The results show that the economically optimal thickness is different for each region analyzed and demonstrates how energy efficiency strategies must be carefully weighed according to the specific conditions of the site.
Water Preservation and Conservation above Coal Mines Using an Innovative Approach: A Case Study
Authors: Xu Yujun , Ma Liqiang , Yu Yihe
Published: June 2020

To better protect the ecological environment during large scale underground coal mining operations in the northwest of China, the authors have proposed a water-conservation coal mining (WCCM) method. This case study demonstrated the successful application of WCCM in the Yu-Shen mining area. Firstly, by using the analytic hierarchy process (AHP), the influencing factors of WCCM were identified and the identification model with a multilevel structure was developed, to determine the weight of each influencing factor. Based on this, the five maps: overburden thickness contour, stratigraphic structure map, water-rich zoning map of aquifers, aquiclude thickness contour and coal seam thickness contour, were analyzed and determined. This formed the basis for studying WCCM in the mining area. Using the geological conditions of the Yu-Shen mining area, the features of caved zone, water conductive fractured zone (WCFZ) and protective zone were studied. The equations for calculating the height of the “three zones” were proposed. Considering the hydrogeological condition of Yu-Shen mining area, the criteria were put forward to evaluate the impact of coal mining on groundwater, which were then used to determine the distribution of different impact levels. Using strata control theory, the mechanism and applicability of WCCM methods, including height-restricted mining, (partial) backfill mining and narrow strip mining, together with the applicable zone of these methods, were analyzed and identified. Under the guidance of “two zoning” (zoning based on coal mining’s impact level on groundwater and zoning based on applicability of WCCM methods), the WCCM practice was carried out in Yu-Shen mining area. The research findings will provide theoretical and practical instruction for the WCCM in the northwest mining area of China, which is important to reduce the impact of mining on surface and groundwater.
Multiparameter and Multiobjective Optimization Design Based on Orthogonal Method for Mixed Flow Fan
Authors: Fan Honggang , Zhang Jinsong , Zhang Wei , Liu Bing
Published: June 2020

Optimization design of an impeller is critical for the energy performance of a fan. This paper takes the promotion of fan efficiency and pressure rise as the optimization objectives to carry out multiparameter and multiobjective optimization research. Firstly, an experimental test bench is built to measure the energy performance of the original fan and verify the accuracy of the numerical method. Then, the hub outlet angle of impeller β1, the impeller outlet angle increment Δβ1, the wrap angle φ, the hub outlet angle of diffuser β2, and the diffuser outlet angle increment Δβ2 are set as the optimal parameters to conduct orthogonal optimal design. The results show that the efficiency of the optimal fan increases by 11.71%, and the pressure rise increases by 50.15%. The pressure and velocity distributions in an optimal fan are uniform, the internal flow separation is weakened, and the influence of tip leakage flow is reduced, which makes for the improvement of energy performance for the fan.
Anaerobic Co-Digestion of Cheese Whey and Industrial Hemp Residues Opens New Perspectives for the Valorization of Agri-Food Waste
Authors: Papirio Stefano , Matassa Silvio , Pirozzi Francesco , Esposito Giovanni
Published: June 2020

Cheese whey (CW) and hemp hurds (HH) represent typically overabundant biowastes of food and agricultural production, and their circular management is crucial to improve both sustainability and profitability of the agri-food chain. By combining experimental biochemical methane potential (BMP) tests and literature data, the techno-economic aspects of a possible future bioenergy valorization of CW and HH through anaerobic digestion (AD) and co- digestion (coAD) were analyzed. Along the 42-days, BMP assays, CW, and HH alone rendered BMP values of 446 ± 66 and 242 ± 13 mL CH4·g VS−1, respectively. The application of coAD with CW and HH at a 70:30 ratio allowed to enhance the biomethane production by 10.7%, as compared to the corresponding calculated value. In terms of economic profitability, the valorization of HH as biomethane in a dual-purpose hemp cultivation could potentially enable net profits of up to 3929 €·ha−1, which could rise to 6124 €·ha−1 in case of coAD with CW. Finally, by projecting the biomethane potential from current and future available CW and HH residues in the national context of Italy, a total biomethane yield of up to 296 MNm3·y−1 could be attained, offering interesting perspectives for the sustainability of key sectors such as transportation.
Evaluation of Waste Plastic Oil-Biodiesel Blends as Alternative Fuels for Diesel Engines
Authors: Kaewbuddee Chalita , Sukjit Ekarong , Srisertpol Jiraphon , Maithomklang Somkiat , Wathakit Khatha , Klinkaew Niti , Liplap Pansa , Arjharn Weerachai
Published: June 2020

This study examined the use of waste plastic oil (WPO) combined with biodiesel as an alternative fuel for diesel engines, also commonly known as compression ignition engines, and focused on comparison of the basic physical and chemical properties of fuels, engine performance, combustion characteristics, and exhaust emissions. A preliminary study was conducted to determine the suitable ratio for the fuel blends in consideration of fuel lubricity and viscosity, and these results indicated that 10% biodiesel—derived from either palm oil or castor oil—in waste plastic oil was optimal. In addition, characterization of the basic properties of these fuel blends revealed that they had higher density and specific gravity and a lower flash point than diesel fuel, while the fuel heating value, viscosity, and cetane index were similar. The fuel blends, comprised of waste plastic oil with either 10% palm oil biodiesel (WPOP10) or 10% castor oil biodiesel (WPOC10), were selected for further investigation in engine tests in which diesel fuel and waste plastic oil were also included as baseline fuels. The experimental results of the performance of the engine showed that the combustion of WPO was similar to diesel fuel for all the tested engine loads and the addition of castor oil as compared to palm oil biodiesel caused a delay in the start of the combustion. Both biodiesel blends slightly improved brake thermal efficiency and smoke emissions with respect to diesel fuel. The addition of biodiesel to WPO tended to reduce the levels of hydrocarbon- and oxide-containing nitrogen emissions. One drawback of adding biodiesel to WPO was increased carbon monoxide and smoke. Comparing the two biodiesels used in the study, the presence of castor oil in waste plastic oil showed lower carbon monoxide and smoke emissions without penalty in terms of increased levels of hydrocarbon- and oxide-containing nitrogen emissions when the engine was operated at high load.
Battery Management Systems—Challenges and Some Solutions
Authors: Balasingam Balakumar , Ahmed Mostafa , Pattipati Krishna
Published: June 2020

Electric vehicles are set to be the dominant form of transportation in the near future and Lithium-based rechargeable battery packs have been widely adopted in them. Battery packs need to be constantly monitored and managed in order to maintain the safety, efficiency and reliability of the overall electric vehicle system. A battery management system consists of a battery fuel gauge, optimal charging algorithm, and cell/thermal balancing circuitry. It uses three non-invasive measurements from the battery, voltage, current and temperature, in order to estimate crucial states and parameters of the battery system, such as battery impedance, battery capacity, state of charge, state of health, power fade, and remaining useful life. These estimates are important for the proper functioning of optimal charging algorithms, charge and thermal balancing strategies, and battery safety mechanisms. Approach to robust battery management consists of accurate characterization, robust estimation of battery states and parameters, and optimal battery control strategies. This paper describes some recent approaches developed by the authors towards developing a robust battery management system.
Urban Design with the Wind: Pedestrian-Level Wind Field in the Street Canyons Downstream of Parallel High-Rise Buildings
Authors: Kuo Chien-Yuan , Wang Rong-Jing , Lin Yi-Pin , Lai Chi-Ming
Published: June 2020

We are investigating how to use urban design approaches to conduct the layout and basic design of newly-built high-rise buildings to reduce wind obstruction and create effective urban ventilation. Few studies have addressed this issue. This study analyzes the effects of high-rise building on pedestrian-level wind in downstream street canyons based on wind tunnel test results, and examines the suitability of an urban design specification. The height (H) of high-rise buildings and the airflow passage width (S) between adjacent high-rise buildings are key control factors for this issue; H/D = 3 and S/D = 1.125 are critical parameters and recommended values (D is the height of the downstream street blocks).
Formation and Continuation of Thermal Energy Community Systems: An Explorative Agent-Based Model for the Netherlands
Authors: Fouladvand Javanshir , Mouter Niek , Ghorbani Amineh , Herder Paulien
Published: June 2020

Energy communities are key elements in the energy transition at the local level as they aim to generate and distribute energy based on renewable energy technologies locally. The literature on community energy systems is dominated by the study of electricity systems. Yet, thermal energy applications cover 75% of the total energy consumption in households and small businesses. Community-driven initiatives for local generation and distribution of thermal energy, however, remain largely unaddressed in the literature. Since thermal energy communities are relatively new in the energy transition discussions, it is important to have a better understanding of thermal energy community systems and how these systems function. The starting point of this understanding is to study factors that influence the formation and continuation of thermal energy communities. To work towards this aim, an abstract agent-based model has been developed that explores four seemingly trivial factors, namely: neighborhood size, minimum member requirement, satisfaction factor and drop-out factor. Our preliminary modelling results indicate correlations between thermal community formation and the ’formation capability’ (the percentage of households that joined) and with the satisfaction of households. No relation was found with the size of the community (in terms of number of households) or with the ‘drop-out factor’ (individual households that quit after the contract time).
Axial-Flux Permanent-Magnet Dual-Rotor Generator for a Counter-Rotating Wind Turbine
Authors: Kutt Filip , Blecharz Krzysztof , Karkosiński Dariusz
Published: June 2020

Coaxial counter-rotating propellers have been widely applied in ships and helicopters for improving the propulsion efficiency and offsetting system reactive torques. Lately, the counter-rotating concept has been introduced into the wind turbine design. Distributed wind power generation systems often require a novel approach in generator design. In this paper, prototype development of axial-flux generator with a counter-rotating field and armature is presented. The design process was composed of three main steps: analytical calculation, FEM simulation and prototype experimental measurements. The key aspect in the prototype development was the mechanical construction of two rotating components of the generator. Sturdy construction was achieved using two points of contact between both rotors via the placement of the bearing between the inner and outer rotor. The experimental analysis of the prototype generator has been conducted in the laboratory at the dynamometer test stand equipped with a torque sensor. The general premise for the development of such a machine was an investigation into the possibility of developing a dual rotor wind turbine. The proposed solution had to meet certain criteria such as relatively simple construction of the generator and the direct coupling between the generator and the wind turbines. The simple construction and the lack of any gearbox would allow for such a system to be constructed relatively cheaply, which is a key aspect in further system development.
The Status of Research and Innovation on Heating and Cooling Networks as Smart Energy Systems within Horizon 2020
Authors: Saletti Costanza , Morini Mirko , Gambarotta Agostino
Published: June 2020

The European Union is funding scientific research through the Horizon 2020 Framework Programme. Since the key priorities for the next few decades are the reduction in carbon emissions and the enhancement of energy system conversion efficiency, a collection of the most recent research projects can be beneficial to researchers and stakeholders who want to easily access and identify recent innovation in the energy sector. This paper proposes an overview of the Horizon 2020 projects on smart distributed energy systems, with particular focus on heating and cooling networks and their efficient management and control. The characteristics of the selected projects are summarized, and the relevant features, including the energy vectors involved, main applications and expected outputs are reported and analyzed. The resulting framework fosters the deployment of digital technologies and software platforms to achieve smart and optimized energy systems.
Evaluation of a Three-Phase Bidirectional Isolated DC-DC Converter with Varying Transformer Configurations Using Phase-Shift Modulation and Burst-Mode Switching
Authors: Mohd Sharifuddin Nuraina Syahira , Tan Nadia M. L. , Akagi Hirofumi
Published: June 2020

This paper presents the performance of a three-phase bidirectional isolated DC-DC converter (3P-BIDC) in wye-wye (Yy), wye-delta (Yd), delta-wye (Dy), and delta-delta (Dd) transformer configurations, using enhanced switching strategy that combines phase-shift modulation and burst-mode switching. A simulation verification using PSCAD is carried out to study the feasibility and compare the efficiency performance of the 3P-BIDC with each transformer configuration, using intermittent switching, which combines the conventional phase-shift modulation (PSM) and burst-mode switching, in the light load condition. The model is tested with continuous switching that employs the conventional PSM from medium to high loads (greater than 0.3 p.u.) and with intermittent switching at light load (less than 0.3 p.u), in different transformer configurations. In all tests, the DC-link voltages are equal to the transformer turns ratio of 1:1. This paper also presents the power loss estimation in continuous and intermittent switching to verify the modelled losses in the 3P-BIDC in the Yy transformer configuration. The 3P-BIDC is modelled by taking into account the effects that on-state voltage drop in the insulated-gate bipolar transistor (IGBTs) and diodes, snubber capacitors, and three-phase transformer copper winding resistances will have on the conduction and switching losses, and copper losses in the 3P-BIDC. The intermitting switching improves the efficiency of the DC-DC converter with Yy, Yd, Dy, and Dd connections in light-load operation. The 3P-BIDC has the best efficiency performance using Yy and Dd transformer configurations for all power transfer conditions in continuous and intermittent switching. Moreover, the highest efficiency of 99.6% is achieved at the light power transfer of 0.29 p.u. in Yy and Dd transformer configurations. However, the theoretical current stress in the 3P-BIDC with a Dd transformer configuration is high. Operation of the converter with Dy transformer configuration is less favorable due to the efficiency achievements of lower than 95%, despite burst-mode switching being applied.
Wireless Power Transfer for Implanted Medical Application: A Review
Authors: Zhou Yujing , Liu Chunhua , Huang Yongcan
Published: June 2020

With ever-increasing concerns on health and environmental safety, there is a fast-growing interest in new technologies for medical devices and applications. Particularly, wireless power transfer (WPT) technology provides reliable and convenient power charging for implant medical devices without additional surgery. For those WPT medical systems, the width of the human body restricts the charging distance, while the specific absorption rate (SAR) standard limits the intensity of the electromagnetic field. In order to develop a high-efficient charging strategy for medical implants, the key factors of transmission distance, coil structure, resonant frequency, etc. are paid special attention. In this paper, a comprehensive overview of near-field WPT technologies in medical devices is presented and discussed. Also, future development is discussed for the prediction of different devices when embedded in various locations of the human body. Moreover, the key issues including power transfer efficiency and output power are addressed and analyzed. All concerning characteristics of WPT links for medical usage are elaborated and discussed. Thus, this review provides an in-depth investigation and the whole map for WPT technologies applied in medical applications.
Complete Data Inventory of a Geothermal Power Plant for Robust Cradle-to-Grave Life Cycle Assessment Results
Authors: Tosti Lorenzo , Ferrara Nicola , Basosi Riccardo , Parisi Maria Laura
Published: June 2020

Technologies to produce electric energy from renewable geothermal source are gaining increasing attention, due to their ability to provide a stable output suitable for baseload production. Performing life cycle assessment (LCA) of geothermal systems has become essential to evaluate their environmental performance. However, so far, no documented nor reliable information has been made available for developing robust LCA studies. This work provides a comprehensive inventory of the Italian Bagnore geothermal power plants system. The inventory is based exclusively on primary data, accounting for every life cycle stage of the system. Data quality was assessed by means of a pedigree matrix. The calculated LCA results showed, with an overall low level of uncertainty (2–3%), that the commissioning and operational phases accounted for more than 95% of the environmental profile. Direct emissions to atmosphere were shown to be the major environmental impact, particularly those released during the operational phase (84%). The environmental performances comparison with the average Italian electricity mix showed that the balance is always in favor of geothermal energy production, except in the climate change impact category. The overall outcome confirms the importance, for flash technology employing fluid with a high concentration of gas content, of using good quality primary data to obtain robust results.
Development of an Interdisciplinary Master of Forestry Program Focused on Forest Management in a Changing Climate
Authors: Walentowski Helge , Kietz Bettina , Horsch Jürgen , Linkugel Thomas , Viöl Wolfgang
Published: June 2020

Concerted efforts are required to achieve the essential UN Sustainable Development Goals (SDGs) of the 2030 Agenda. This concept paper is focused on the development of a new Master of Forestry (MF) degree program at our University of Applied Sciences (UAS). With this move, we want to outline how capacity building and valuable synergy effects can be obtained from close cooperation in teaching and research, in order to educate our scientifically trained and practice-oriented forestry students in applying new management responses to natural disturbance impacts and sustainable use of terrestrial natural resources for forest resource-dependent communities. Specifically, we show how the emergent issues of global warming and the associated increased vulnerability of temperate deciduous forests can be tackled. Actions to overcome knowledge transfer barriers will provide sound solutions for SDG 4 (quality education), SDG 5 (gender equality), SDG 7 (affordable and clean energy), SDG 12 (responsible consumption and production), SDG 13 (climate action), SDG 15 (life on land), and SDG 17 (partnerships to achieve the goal). Focusing on the Global Sustainable Development Goals can trigger intra and inter-faculty processes of cooperation, exchange programs, and optimized interfaces of previously separated disciplines that complement each other perfectly to form a knowledge hub.
Metabolism of Lactobacillus sakei Chr82 in the Presence of Different Amounts of Fermentable Sugars
Authors: Barbieri Federica , Laghi Luca , Gardini Fausto , Montanari Chiara , Tabanelli Giulia
Published: June 2020

Lactobacillus sakei is widely used as a starter culture in fermented sausages since it is well adapted to meat environments and able to maintain high viability thanks to secondary pathways activated when hexoses are depleted (i.e., metabolism of pentoses and amino acids). In this study, a commercial strain of L. sakei was inoculated in a defined medium with ribose or glucose as the carbon source, at optimal or reduced concentrations, to evaluate its different physiological and metabolic responses in relation to different growth conditions. The results obtained with different approaches (HPLC, 1H-NMR, flow cytometry) evidenced different growth performances, amino acid consumptions and physiological states of cells in relation to the carbon source as an active response to harsh conditions. As expected, higher concentrations of sugars induced higher growth performances and the accumulation of organic acids. The low sugars amount induced the presence of dead cells, while injured cells increased with ribose. Arginine was the main amino acid depleted, especially in the presence of higher ribose, and resulted in the production of ornithine. Moreover, the 1H-NMR analysis evidenced a higher consumption of serine at the optimal sugars concentration (pyruvate production). This information can be helpful to optimize the use of these species in the industrial production of fermented sausages.
Turrón Coproducts as Source of Bioactive Compounds: Assessment of Chemical, Physico-Chemical, Techno-Functional and Antioxidant Properties
Authors: Lorente-Mento José Manuel , Lucas-González Raquel , Sayas-Barbera Estrella , Pérez-Álvarez José Ángel , Fernández-López Juana , Viuda-Martos Manuel
Published: June 2020

The goals of this research were determined the proximate composition, physico-chemical, techno-functional properties, the polyphenolic profile, the organic acids and sugar content and the antioxidant capacities of flours obtained from almonds skins var. comuna (ASFC) and var. fritz (ASFF) coproducts produced in Turrón industry. The chemical composition and physico-chemical properties (pH, water activity and color) were determined. The water holding, oil holding and swelling capacities were also determined, as well as the polyphenolic profile. For the antioxidant capacity, four different assays were used namely: 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay (DPPH•); Ferrous ions chelating activity (FIC); Ferric reducing antioxidant power (FRAP) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assay (ABTS•+). The flours obtained from ASFC and ASFF had a high content of dietary fiber (70.50 and 69.83 g/100 g, respectively). The polyphenolic profile, determined by High Performance Liquid Chromatography, identified 21 and 19 polyphenolic compounds in both ASFC and ASFF, being epicatechin and catechin the most abundant compounds. In reference to the antioxidant capacity regards, with all methods assayed except FRAP, ASFC had higher antioxidant activity than ASFF. These coproducts show good technological and antioxidant properties, which makes them a good alternative for its use in the development of new foods.
Antibiotic Resistance in Minimally Human-Impacted Environments
Authors: Scott Laura C. , Lee Nicholas , Aw Tiong Gim
Published: June 2020

Antibiotic resistant bacteria (ARB) have become contaminants of concern in environmental systems. Studies investigating environmental ARB have primarily focused on environments that are greatly impacted by anthropogenic activity. Background concentrations of ARB in natural environments is not well understood. This review summarizes the current literature on the monitoring of ARB and antibiotic resistance genes (ARGs) in environments less impacted by human activity. Both ARB and ARGs have been detected on the Antarctic continent, on isolated glaciers, and in remote alpine environments. The methods for detecting and quantifying ARB and ARGs from the environment are not standardized and warrant optimization. Further research should be focused on the detection and quantification of ARB and ARGs along human gradients to better characterize the factors leading to their dissemination in remote environments.
Twelve Weeks of Whole Body Vibration Training Improve Regucalcin, Body Composition and Physical Fitness in Postmenopausal Women: A Pilot Study
Authors: Pérez-Gómez Jorge , Adsuar José Carmelo , García-Gordillo Miguel Ángel , Muñoz Pilar , Romo Lidio , Maynar Marcos , Gusi Narcis , P. C. Redondo
Published: June 2020

(1) Background: Regucalcin or senescence marker protein 30 (SMP30) is a Ca2+ binding protein discovered in 1978 with multiple functions reported in the literature. However, the impact of exercise training on SMP30 in humans has not been analyzed. Aging is associated with many detrimental physiological changes that affect body composition, functional capacity, and balance. The present study aims to investigate the effects of whole body vibration (WBV) in postmenopausal women. (2) Methods: A total of 13 women (aged 54.3 ± 3.4 years) participated in the study. SMP30, body composition (fat mass, lean mass, and bone mass) and physical fitness (balance, time up and go (TUG) and 6-min walk test (6MWT)) were measured before and after the 12 weeks of WBV training. (3) Results: The WBV training program elicited a significant increase in SPM30 measured in plasma (27.7%, p = 0.004) and also in 6MWT (12.5%, p < 0.001). The WBV training also significantly reduced SPM30 measured in platelets (38.7%, p = 0.014), TUG (23.1%, p < 0.001) and total body fat mass (4.4%, p = 0.02). (4) Conclusions: There were no significant differences in balance, lean mass or bone mass. The present study suggests that 12 weeks of WBV has the potential to improve SPM30, fat mass, TUG and 6MWT in postmenopausal women.
Behavioral Inhibition in Childhood as A Risk Factor for Development of Social Anxiety Disorder: A Longitudinal Study
Authors: Luis-Joaquin Garcia-Lopez , Lourdes Espinosa-Fernández , José A Muela-Martínez
Published: June 2020

Previous research has suggested the association between behavioral inhibition (BI) and the development of social anxiety disorder in childhood. However, there is scarce research using longitudinal methodology in Spanish-speaking populations. To cover this gap, the sample comprised 73 children ranging from six to eight years who had been examined for BI two years earlier in home and school settings. Children and their parents were administered the Anxiety Disorders Interview Schedule for DSM-5-Child and Parent Versions to assess the presence of possible anxiety disorders. The results revealed the stability of BI symptomatology over time. Data also showed that BI children were almost ten times more likely to develop social anxiety disorder two years later, compared to no-BI children. As a result, findings suggest behavioral inhibition strongly predicts social anxiety disorder, making BI a logical focus for selective preventive interventions. Therefore, screening for behavioral inhibition holds promise for primary prevention.
Methodology to Prioritize Chilean Tailings Selection, According to Their Potential Risks
Authors: Lam Elizabeth J. , Montofré Italo L. , Álvarez Fernando A. , Gaete Natalia F. , Poblete Diego A. , Rojas Rodrigo J.
Published: June 2020

For centuries, Chile has been a territory with significant mining activity, resulting in associated social benefits and impacts. One of the main challenges the country faces today is the presence of a great number of mine tailings containing heavy metals, such as Cu, Cr, Ni, Zn, Pb, As, Cd, and Fe, which make up a potential risk for the population. This study is intended to develop a methodology for determining tailings requiring urgent treatment in Chile, based on risks associated with heavy metals. Geochemical data from 530 Chilean tailings were compared to the Dutch norm and the Canadian and Australian soil quality guidelines for residential use. Additionally, criteria about residents and water bodies were used, considering a 2-km area of influence around tailings. To do this, QGIS (Böschacherstrasse 10a CH-8624 Grüt (Gossau ZH), Zurich, Switzerland), a geospatial tool, was used to geolocate each deposit, considering regions, communes, rivers, lakes, and populated areas. To evaluate potential ecological contamination risks, Hakanson’s methodology was used. Results revealed the presence of 12 critical tailings in Chile that require urgent treatment. From the 530 tailings evaluated, 195 are located at less than 2 km from a populated area and 154 at less than 2 km from a water body. In addition, 347 deposits require intervention: 30 on Cu, 30 on Cr, 13 on Zn, 69 on Pb, 138 on As, 1 on Cd, and 5 on Hg.
Characterization of miRNAs in Cultured Atlantic Salmon Head Kidney Monocyte-Like and Macrophage-Like Cells
Authors: Smith Nicole C. , Christian Sherri L. , Woldemariam Nardos T. , Clow Kathy A. , Rise Matthew L. , Andreassen Rune
Published: June 2020

Macrophages are among the first cells to respond to infection and disease. While microRNAs (miRNAs) are involved in the process of monocyte-to-macrophage differentiation in mammals, less is known in teleost fish. Here, Atlantic salmon head kidney leukocytes (HKLs) were used to study the expression of miRNAs in response to in vitro culture. The morphological analysis of cultures showed predominantly monocyte-like cells on Day 1 and macrophage-like cells on Day 5, suggesting that the HKLs had differentiated from monocytes to macrophages. Day 5 HKLs also contained a higher percentage of phagocytic cells. Small RNA sequencing and qPCR analysis were applied to examine the miRNA diversity and expression. There were 370 known mature Atlantic salmon miRNAs in HKLs. Twenty-two miRNAs (15 families) were downregulated while 44 miRNAs (25 families) were upregulated on Day 5 vs. Day 1. Mammalian orthologs of many of the differentially expressed (DE) miRNAs are known to regulate macrophage activation and differentiation, while the teleost-specific miR-2188, miR-462 and miR-731 were also DE and are associated with immune responses in fish. In silico predictions identified several putative target genes of qPCR-validated miRNAs associated with vertebrate macrophage differentiation. This study identified Atlantic salmon miRNAs likely to influence macrophage differentiation, providing important knowledge for future functional studies.
AtSK11 and AtSK12 Mediate the Mild Osmotic Stress-Induced Root Growth Response in Arabidopsis
Authors: Dong Long , Wang Zhixin , Liu Jing , Wang Xuelu
Published: June 2020

Although most osmotic stresses are harmful to plant growth and development, certain drought- or polyethylene glycol (PEG)-induced mild osmotic stresses promote plant root growth. The underlying regulatory mechanisms of this response remain elusive. Here, we report that the GLYCOGEN SYNTHASE KINASE 3 (GSK3) genes ARABIDOPSIS THALIANA SHAGGY-RELATED KINASE 11 (AtSK11) (AT5G26751) and AtSK12 (AT3G05840) are involved in the mild osmotic stress (−0.4 MPa) response in Arabidopsis thaliana. When grown on plant medium infused with different concentrations of PEG to mimic osmotic stress, both wild-type (WT) and atsk11atsk12 plants showed stimulated root growth under mild osmotic stress (−0.4 MPa) but repressed root growth under relatively strong osmotic stress (−0.5, −0.6, −0.7 MPa) as compared to the mock condition (−0.25 MPa). The root growth stimulation of atsk11atsk12 was more sensitive to −0.4 MPa treatment than was that of WT, indicating that AtSK11 and AtSK12 inhibit the mild stress-induced root growth response. RNA-seq analysis of WT and atsk11atsk12 plants under three water potentials (−0.25 MPa, −0.4 MPa, −0.6 MPa) revealed 10 differentially expressed candidate genes mainly involved in cell wall homeostasis, which were regulated by AtSK11 and AtSK12 to regulate root growth in response to the mild stress condition (−0.4 MPa). Promoter motif and transcription factor binding analyses suggested that the basic helix-loop-helix (bHLH) transcription factor bHLH69/LJRHL1-LIKE 2 (LRL2) may directly regulate the expression of most −0.4 MPa-responsive genes. These findings indicate that mild osmotic stress (−0.4 MPa) promotes plant growth and that the GSK3 family kinase genes AtSK11 and AtSK12 play a negative role in the induction of root growth in response to mild osmotic stress.
The Osmotin-Like Protein Gene PdOLP1 Is Involved in Secondary Cell Wall Biosynthesis during Wood Formation in Poplar
Authors: Li Shaofeng , Zhang Yaoxiang , Xin Xuebing , Ding Changjun , Lv Fuling , Mo Wenjuan , Xia Yongxiu , Wang Shaoli , Cai Jingyan , Sun Lifang , Du Manyi , Dong Chenxi , Gao Xu , Dai Xinlu , Zhang Jianhui , Sun Jinshuang
Published: June 2020

Osmotin-like proteins (OLPs) mediate defenses against abiotic and biotic stresses and fungal pathogens in plants. However, no OLPs have been functionally elucidated in poplar. Here, we report an osmotin-like protein designated PdOLP1 from Populus deltoides (Marsh.). Expression analysis showed that PdOLP1 transcripts were mainly present in immature xylem and immature phloem during vascular tissue development in P. deltoides. We conducted phenotypic, anatomical, and molecular analyses of PdOLP1-overexpressing lines and the PdOLP1-downregulated hybrid poplar 84K (Populus alba × Populus glandulosa) (Hybrid poplar 84K PagOLP1, PagOLP2, PagOLP3 and PagOLP4 are highly homologous to PdOLP1, and are downregulated in PdOLP1-downregulated hybrid poplar 84K). The overexpression of PdOLP1 led to a reduction in the radial width and cell layer number in the xylem and phloem zones, in expression of genes involved in lignin biosynthesis, and in the fibers and vessels of xylem cell walls in the overexpressing lines. Additionally, the xylem vessels and fibers of PdOLP1-downregulated poplar exhibited increased secondary cell wall thickness. Elevated expression of secondary wall biosynthetic genes was accompanied by increases in lignin content, dry weight biomass, and carbon storage in PdOLP1-downregulated lines. A PdOLP1 coexpression network was constructed and showed that PdOLP1 was coexpressed with a large number of genes involved in secondary cell wall biosynthesis and wood development in poplar. Moreover, based on transcriptional activation assays, PtobZIP5 and PtobHLH7 activated the PdOLP1 promoter, whereas PtoBLH8 and PtoWRKY40 repressed it. A yeast one-hybrid (Y1H) assay confirmed interaction of PtoBLH8, PtoMYB3, and PtoWRKY40 with the PdOLP1 promoter in vivo. Together, our results suggest that PdOLP1 is a negative regulator of secondary wall biosynthesis and may be valuable for manipulating secondary cell wall deposition to improve carbon fixation efficiency in tree species.
Clinical Usefulness of Susceptibility Breakpoints for Yeasts in the Treatment of Candidemia: A Noninterventional Study
Authors: Lass-Flörl Cornelia , Krause Robert , Willinger Birgit , Starzengruber Peter , Decristoforo Petra , Neururer Sabrina , Kreidl Peter , Aigner Maria
Published: June 2020

This prospective noninterventional study evaluated whether antifungal susceptibility data (MIC) provided for Candida clinical isolates on the basis of recently established breakpoints are taken into account by clinicians to guide their treatment decision making process, and assessed the response in MIC- and non-MIC-based treatment groups. During a six month period, the usage of systemic antifungals was recorded in detail and compared with mycological data (Candida species and MICs) in candidemia patients. Patients were assigned to a susceptible or resistant infection group based on European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints; treatment decisions were under the professional discretion of the treating physicians. 123 patients were evaluated with Candida albicans accounting for 59%, Candida glabrata for 19%, Candida parapsilosis for 15%, Candida tropicalis for 4% and Candida krusei for 3%. Antifungal treatment correlated with species and MICs in 80% (n = 99 patients), high MICs and species-dependent guideline recommendations were ignored in 20% (n = 24 patients); the overall outcome of candidemia cases in our study population was excellent, as by day 14, all patients were cleared from fungal blood stream infection (mean 5.6 days, range 2–12). The current variability in antifungal usage and the delay in initiating appropriate therapy indicate a need for antifungal stewardship to improve the management of invasive fungal infections.
Transport Theorem for Spaces and Subspaces of Arbitrary Dimensions
Authors: Jaric Jovo P. , Vignjevic Rade , Mesarovic Sinisa Dj.
Published: June 2020

Using the apparatus of traditional differential geometry, the transport theorem is derived for the general case of a M-dimensional domain moving in a N-dimensional space, M ≤ N . The interesting concepts of curvatures and normals are illustrated with well-known examples of lines, surfaces and volumes. The special cases where either the space or the moving subdomain are material are discussed. Then, the transport at hypersurfaces of discontinuity is considered. Finally, the general local balance equations for continuum of arbitrary dimensions with discontinuities are derived.