bluuebunny/crossref_metadata_2025 huggingface dataset:
Local Cellular Immune Response in Dysplastic Epithelial Lesions of the Flat Colonic Mucosa
Authors: Radović Svjetlana , Selak Ivan , Bratović I.
Published: May 2018

This is an immunohistochemical study of the local cellular immune response characteristic in inflammatory-regenerative and dysplastic flat colonic mucosa. The aim of this study is to determine a possible existence of the mononuclear cellular infiltration specificity which could be important for the prognosis in further development of dysplastic lesion. Biopsy specimens from 170 patients (specimens stained by hematoxylin eosin) were examined. 74 specimens showed inflammatory-regenerative changes and 96 had dysplastic changes (38 with mild dys-plasia, 28 with moderate dysplasia and 30 with severe dysplasia). Three monoclonal antibodies were used for the identification of mononuclear cells in the inflammatory cellular infiltration in the lamina propria of colonic mucosa. The inflammatory cells type and their location in respect of the epithelial cells and lesion itself were analysed and their number was determined by the semi-quantitative method. T lymphocytes were the dominant cells of local immune response in dysplastic lesions while macrophages were less present and B lymphocytes, as rare cells, were present in sporadic cases. It is notified that increase in the extent of dysplasia was followed by increase in the number of macrophages and T lymphocytes in particular. Immediate contact between macrophages and T lymphocytes in epithelial dysplasia was found in a small number of cases and was mostly independent from the intensity of dysplasia. Signs of the direct lytical effect of the mononuclear ceils on dysplastic epithelial cells were not observed in this contact. It seems that epithelial dysplasia does not provoke more significant local immune response which is the most probably a part of the chronic non-specific inflammation that has a negative influence on further development of the lesion. The conclusion is that local immune response in the dysplastic alteration of flat colonic mucosa has no importance in further prognosis of the lesion.
Erythrocyte D -Aminolevulinic Acid Dehydratase (Ala-D) Activity and Blood Lead Level (Pb-B) of Tortoise (Testudo Hemanni, Gmel.) of Vicinity of Lead and Zinc Smelter “Trepca” in Kosova
Authors: Elezaj I. , Rozhaja D.A.
Published: May 2018

In comparison with control animals, a significant negative correlation (r = 0.77 -), between lead level and the activity of daminolevulinic acid dehydratase activity in the blood of land turtle (Testudo hermanni, Gmel.) caught in five different localities from heavy polluted environment of lead and zinc smelter “Trepca” was found. The concentration ratio of lead level in blood between control animals and those from the vicinity of smelter was 10:160 % and ratio of ALA-D activity was 10:18,3 U/LE.
Mathematical methods for quantification and comparison of dissolution testing data
Authors: Vranić Edina , Mehmedagić Aida , Hadžović Sabira
Published: May 2018

In recent years, drug release/dissolution from solid dosage forms has been the subject of intense and profitable scientific developments. Whenever a new solid dosage form is developed or produced, it is necessary to ensure that drug dissolutionoccurs in an appropriate manner. The pharmaceutical industry and the registration authorities do focus, nowadays, on drug dissolution studies. The quantitative analysis of the values obtained in dissolution/release tests is easier when mathematicalformulas that express the dissolution results as a function of some of the dosage forms characteristics are used. This work discusses the analysis of data obtained for dissolution profiles under different media pH conditions using mathematical methodsof analysis described by Moore and Flanner. These authors have described difference factor (f1) and similarity factor (f2), which can be used to characterise drug dissolution/release profiles. In this work we have used these formulas for evaluation of dissolution profiles of the conventional tablets in different pH of dissolution medium (range of physiological variations).
Frequency of rh phenotypes in relation to the outcome of pregnancy in the two groups of pregnant women
Authors: Redžić Amira , Begić Fatima
Published: May 2018

Lethal and sublethal genetical factors, including Rh factor, represent endogenous risk factors of the pregnancy outcome. These factors are most frequently inherited in recessive way and they often lead to the negative outcome of pregnancies. They represent pregnancy (a prirori) risk of various degrees. Inheritance of Rh system blood groups is linked to chromosome 1 and it could be explained by two alternative theories; molecular Rh system genetics has not yet been completely explained. The first formal-genetic theory postulates three closely linked gene sites (loci C, D and E) while the second theory has a monogenic character (one locus with several allele genes). Data on 755 pregnancies, which were (for various reasons) estimated as increased risk pregnancies, were registered at Gynaecology Clinic, Clinical Centre of University of Sarajevo, during the period from 1989 to 1992. These data were collected from pregnant women who, according to the certain indications from their familiar and personal anamnesis, demanded genetic consultations. The result of investigation of the basic Rh system phenotype distribution shows no statistically significant difference between monitored pregnant women. This result is assumed as valid for both pregnant women and their partners. The same result is suggesting that the observed increased risk pregnancy samples do not significantly differ from the previously studied population samples. Therefore, it has been concluded that Rh factor is not closely related to the increased risk of individual pregnancy outcomes, that is, it does not have relevant influence on the observed reproduction parameters. This result is very interesting and deserves particular medical attention and further evaluation in the future, particularly considering known immunological phenomena resulting from relations between reproduction partners belonging to the basic Rh system phenotypes.
Oral acute toxicity of HEPALIP FORTE in rats
Authors: Todić Maida , Bakić Sanela , Zulić Irfan , Krošnjar Sanja , Begović Begler , Dorić Mirsad , Babić Mirsad
Published: May 2018

he main active component of preparation HEPALIP FORTE is EPL--essential phospholipids. Their chemical structure corresponds to that of endogen phospholipids, but they have functional superiority because of the content of unsaturated fatty acids. Essential phospholipids in combination with the vitamins have been used in the treatment of liver diseases, dyslipoproteinaemias and intoxications with consequent liver failure. Acute toxicity study on HEPALIP FORTE was performed on Wistar rats. The main aim of toxicology studies for the drug registration process is evaluation of the toxic potential and risks of human exposition to the substance (Gelbke et al., 1999). Acute toxicity is an orientation point of the test substance toxicity and represents a starting test for the toxicological evaluation. Study included one oral dose of the substance, applied with oesophageal intubations. There were three dose-levels: 300, 500 and 1000 mg/kg. No lethality was recorded and statistical analysis of body weight variations failed to show any significant difference between the groups. Reversible tremor was more frequently recorded in females and was not present in control animals. After the planed sacrifice, no changes related to the test substance were recorded. We noticed a statistically significant difference in the liver weights between males of 3M and 2M groups in comparison to the control. Similar (not significant) tendency was noticed in females. Significant differences in organ weights might be suggestive of a toxic effect that experimental animal managed to recover from in partial manner. The histopathological analysis detected no changes in the structure and morphology of liver parenchyma.
Food and water consumption in assessment of acute oral toxicity of HEPALIP FORTE in rats
Authors: Todić Maida , Bakić Sanela , Begović Begler , Krošnjar Sanja , Zulić Irfan
Published: May 2018

Body weight variations during toxicological testing can be one of the indicators of the test substance toxic effects. Data on food and water consumption are true indicators of the rate of growth of experimental animals (Stevens & Gallo, 1989). Daily recording of the food and water consumption was done during the acute toxicity testing of HEPALIP FORTE. The study was performed on Wistar rats. The active component of HEPALIP FORTE is EPL substance--essential phospholipids, a natural substance present in every living cell. Essential phospholipids in combination with vitamins have been used in the treatment of liver diseases, dyslipoproteinaemias and intoxications accompanied with liver failure. Statistical analysis of the body weight variations was performed separately, for males and females. The analysis failed to show any significant difference between the groups. There was a significant difference in water consumption between the male group 2M and female groups 3F and 2F in comparison with control groups. Statistical analysis of the variations of food consumption showed a significant difference in all male groups in comparison with control groups, and only in the 3F female group in comparison with a control group. Considering the absence of lethality and the lack of significant influence of the test substance on animal body weights, we concluded that the test substance was not acutely toxic in rats, if applied orally, in single doses of 300 mg/kg, 500 mg/kg and 1000 mg/kg. Significant differences found in food and water consumption suggest a need of their during the future chronic toxicity testing.
Editorial
Authors: Board Editorial
Published: May 2018

We would like to inform our readers that Association of Basic Medical Sciences, beginning with this year, will publish Bosnian Journal of Basic Medical Sciences at least quarterly, depending of your submissions.As it is previously announced, the second opening lecture form of the International and Scientific Symposium about human embryo and its cloning held in Sarajevo, 15 February 2003 (“Scientific, ethical and religious dilemmas over the status of human embryo and its cloning”) is published in this issue.In this issue, in concordance with the current world situation and after the suggestions of some of our distinguish colleagues, we decide to publish a review article on aetiology and epidemiology of Severe Acute Respiratory Syndrome (SARS) -a new emerging disease.We kindly remind our readers to inform other colleagues and associates about the Association and our membership.All observation, suggestions, and proposals from our readers are more then welcome and will improve the quality of the Journal.We are inviting scientists from not only entire Bosnia and Herzegovina but internationally, to participate in next issues and hope that many of them will answer our invitation.Sarajevo, May, 2003Editorial Board
Brunkow exercises and low back pain
Authors: Mujić Skikić Emela , Trebinjac Suad , Sakota Slavica , Avdić Dijana , Delić Aida
Published: May 2018

Brunkow exercises starting with dynamic contraction of hands and feet with fixed point on the wrist or/and heal. Dynamic contraction from the beginning, transferring through kinetic chain, leads to isometric contraction of the group of muscles, which has to be included in the exercise. Starting positions determine the group of muscles to be trained. The purpose of this study is to investigate influence of Brunkow exercises on spinal motion improvement and pain relief and to evaluate use of Brunkow exercises, as a routine method for lower back pain in Physical Medicine and Rehabilitation Centres. Thirty-four patients with symptoms of low back pain were included in study. Patients received a mean of 14.9 treatments with standard deviation of 8.96. All patients were assessed before and after the treatment for spinal mobility and flexibility as well as pain intensity. All parameters for spinal movements showed statistically significant improvement in patients with low back pain who practiced Brunkow exercise program at the end of treatment in relations to pre-treatment values, with significant difference of p<0.01 for all motions. Pain was reduced on VAS for X=1.,7 with S.D. 1.97. Difference Test was t=6.020 with significant difference p<0.01. Flexibility of spine increased, so average difference in values before and after treatment for Shober test was 0.5 cm with SD 0.65. Difference test was t=3.794 with significant difference p<0.01. Brunkow exercises for low back pain are beneficial treatment for increasing flexibility and mobility of spine and improving the pain.
Contribution to the knowledge of position, flow and arterial distribution of cerebral blood vessels in foetuses 4 to 9 months of age
Authors: Kulenović Amela , Dilberović Faruk
Published: May 2018

We studied cerebral blood vessels in 25 fetuses of gestational age 16-36 weeks and in 10 cadavers of still-born babies by injection-corrosive method. In the early fetal life, arteries are thin with the straight flow, which is directly connected with the brain development. Progressive changes are observed in all the three cerebral arteries in 28-week old fetus, which straight flow becomes more and more tortuous. As in the 32nd week the brain develops faster and gyri and sulci are being formed, the arteries assume wavy flow and number of their rami increases. In a still-born baby, arteries are of rather bigger caliber; they branch abundantly; and due to their relatively broad cerebral sulci, it can be said that their flow is partly tortuous. Our results show evidently that position, flow and relation of cerebral arteries change concurrently with the brain development and appearance of cerebral gyri and sulci.
Endometrial cancer epidemiology and prevention in Federation of Bosnia and Herzegovina, B&H
Authors: Vasilj Ivan , Cavaljuga Semra , Strnad Marija , Znaor Ariana
Published: May 2018

In Federation of Bosnia and Herzegovina during 2002 a total of 67 cases of endometrial cancer (ICD 10th Revision Code C54) were registered among female population older then 15 years (1 per 10,000 population). Nine women were diagnosed with non specific malignant uteri neoplasia (C55)--without clarifying if that was cervix or corpus uteri located cancer, but assumption is that these cancers are actually endometrial cancer. Majority of cases are older then 50 years, 48 of them (71.6%), while 29 (28.4%) are from 15 to 49 years old. During 2000 about 189,000 new endometrial cancer cases were reported with 44,700 endometrial cancer deaths in the World. In this paper we presented geographical distribution of cases registered in FB&H, as well as leading risk factors, protective factors and prevention and possibilities for screening methods.
Editorial
Authors: Mujic Muzafer
Published: May 2018

We inform our readers and collaborators that the interest for publication in Bosnian Journal of Basic Medical Sciences is growing continuously. Numerous letters and messages (e-mail) of support, in particular from our scientists working abroad, are sufficient confirmation. This exchange resulted in the idea (E. Kanlic, MD PhD, Associate Professor, Dept. of Orthopedic, Texas) that our scientists working in the USA edit one issue (preliminary deadline in April 2005) that would be dedicated to the latest understandings and achievements in a specific area that would be of interest for our experts. We are looking forward to successful cooperation with other colleagues engaged in fundamental science.It is with great pleasure that we inform you that, after anticipating a reply from the Department of Health and Human Services for some time, on the 6th July 2004 we received an information from Mr. Sheldon Kotzin, Executive Editor MEDLINE/Index Medicus stating that Bosnian Journal of Basic Medical Sciences has received a positive score (very good) upon reviewing and has been accepted for indexation in MEDLINE/Index MedicusWe are proud that our issues are mainly filled with papers by young researchers which agrees with our primary intention that the Journal grows into a vehicle for the exchange of knowledge among young researchers in the world. With this issue we complete the cycle for this year. We are convinced that, despite significant difficulties, we will find adequate support and ensure regular publication of research results from the entire Bosnia and Herzegovina.With sincere greetings and gratitude,Sarajevo, October 2004Editorial and Advisory BoardM.MujicThis Journal is Indexed in: CAB Abstract / Global Health databases and Index Medicus/MEDLINE.
Respiratory risk factors in development of postoperative complications after the lung resection
Authors: Krdžalić Goran , Kabil Emir , Salaka Urnid , Sijerčić Mirna , Krdžalić Alisa
Published: May 2018

The objectives of the study were to identify possible associated respiratory risk factors and to assess incidence of overall postoperative complications after the lung resection. We reviewed 110 patients who underwent lung resections due to malignant neoplasms or benign lung diseases. The risk of postoperative complications was evaluated using the univariate analysis. Results confirmed that low FEV1, postoperative high PaCO2, ASA-status and advanced age were factors associated with development of postoperative complications.
Very high resolution chemical imaging with Infrared Scanning Near-field Optical Microscopy (IR-SNOM)
Authors: Vobornik Dušan , Margaritondo Giorgio , Vobornik Slavenka , Thielen Peter , Generosi Renato , Tolk Norman , Cricenti Antononio
Published: May 2018

In this paper we present chemically highly resolved images obtained with Scanning Near-field Optical Microscopy (SNOM) coupled with an Infrared (IR) Free Electron Laser (FEL) at Vanderbilt University, Nashville, USA. Main principles governing SNOM imaging as well as essential components of the experimental setup are described. Chemically resolved images showing the distribution of different phases within the boron-nitride films are presented. Universal character of the experiment and its huge potential applications in biophysics and medical sciences domain are illustrated with highly resolved SNOM images of pancreatic cells.
Different digital imaging techniques in dental practice
Authors: Lačević Amela , Vranić Edina
Published: May 2018

Different imaging techniques are used to pick up the signal of interest in digital sensors, including charge-coupled devices (CCD), complementary metal-oxide semiconductors (CMOS), photostimulable phosphors plates (PSP) and tuned-aperture computed tomography (TACT) Digital radiography sensors are divided into: storage phosphor plates (SPP) called photostimulable phosphor plates (PSP), silicon devices such as charge-coupled devices (CCD) or complementary metal oxide semiconductors (CMOS).Relatively new type of imaging that may hold advantage over current radiographic modalities is tuned-aperture computed tomography (TACT).
Basic principles of drug--excipients interactions
Authors: Vranić Edina
Published: May 2018

Excipients are generally considered inert additives included in drug formulation to help in the manufacturing, administration or absorption. Other reasons for inclusion concern product differentiation, appearance enhancement or retention of quality. Excipients can initiate, propagate or participate in chemical or physical interactions with an active substance, possibly leading to compromised quality or performance of the medication. Understanding the chemical and physical nature of excipients, the impurities or residues associated with them and how they may interact with other materials, or with each other, forewarns the pharmaceutical technologist of possibilities for undesirable developments.
Editorial
Authors: Board Editorial
Published: May 2018

The first edition of the Bosnian Journal of Basic Medical Sciences in the year 2004 is a great opportunity for the Editorial Board to authenticate its complete successfulness in realisation of the intentions to progressively achieve the major aim: to issue one edition every three months or four editions per a year.In addition, there is an increasing number of scientists being interested to publish the results of their investigations in our journal. We also need to emphasise, that an augmented number of authors from the foreign countries are attracted by the scope of the journal.In this edition, we issue the rest of scientific papers about cloning, which were presented during the Symposium in Sarajevo.The Association of Basic Medical Sciences has performed necessary additions in its Presidency, Editorial Board and Advisory board.We are proud to mention that we have daily contacts with younger native and foreign scientists interested in collaboration within our journal.We take in consideration all suggestions with purpose to improve the quality of the journal. In addition, we are grateful for all of your opinions, advices and recommendations that we constantly try to accept and implement.Sarajevo, Februar, 2004Editorial Board
The facts and doubts about beginning of the human life and personality
Authors: Kurjak Asim , Tripalo Ana
Published: May 2018

“What a piece of work is a man!” William Shakespeare, Hamlet“To those of average curiosity about the wonders of nature, it is likely that two great mysteries have stirred the imagination; and each concerns a birth. Who has not gazed into the heavens on the starlit night and wondered about the birth of the universe? And who has not been stimulated by the sight of the newly born baby to the marvel at the unseen events within the mother’s uterus that have led to the birth of such a perfect creation?”(1) These words written by the Professor Sir Graham (Mont) Liggins open Pandora’s box of questions, dilemmas, doubts and controversies about human life and its beginning offering everybody lifelong challenge to solve mystery of life.Entering this filed scientists have been remiss in failing to translate science into the terms that allow mankind to share their excitement of discovering life before birth. Regardless to remarkable scientific development, curiosity, and speculations dating back to Hippocrates, life before birth still remains a big secret. Different kinds of intellectuals involved themselves trying to contribute to the solution of human life puzzle. They are led by the idea that each newborn child will only reach its full potential if its development in uterus is free from any adverse influence, providing the best possible environment for the embryo/foetus. Considering embryo/foetus, it should be always kept in mind amazing aspect of these parts of human life in which the mother and the embryo/foetus, although locked in the most intimate relationships, are at ALL TIMES two separate people. Accepting embryo/foetus as the person opened new set of questions about its personality and human rights. Today, synthesis between scientific data and hypotheses, philosophical thought, and issues in the humanities, has become pressing necessity in order to deal with ethical, juridical and social problems arising from man’s interference in many aspects and stages of life. (2)
Demonstration of different endocervical staining methods and their usefulness in the diagnosis of the chlamydial infection in exfoliated cells advantages and disadvantages
Authors: Mahmutović Sabina , Bešlagić Edina , Hamzić Sadeta , Aljicević Mufida
Published: May 2018

Microscopic demonstration of chlamydial inclusions within cells offered the first laboratory procedure supporting the clinical diagnosis of chlamydial infection. Our aim is to evaluate the usefulness of different endocervical staining methods in diagnosis of Chlamydia trachomatis (CT) infection within exfoliated cells of the endocervix. The cytological test for the detection of chlamydial inclusions in genital tract infection, though not as sensitive and specific as isolation in the cell culture monolayers, is still of the diagnostic value. The present study discusses the collection of clinical smears for microscopic examination, their preparation; fixation and staining of slides by a variety of staining methods that have been used to detect Chlamydia in clinical smears and biopsies. Most of these methods such as Giemsa stain, Papanicolaou, iodine, and immunofluorescence (IF) using monoclonal antibodies, are based on the combination of dyes designed to obtain optimum differentiation of the various structures. The utilization of different endocervical smear stains together with the clinical information can be used to identify women at high risk for CT infection.
Posttraumatic stress disorder among women after the war in Sarajevo: a rationale for genetic study.
Authors: Oruč Lilijana , Kapur Lejla , Pojskić Naris , Čavaljuga Semra , Ivezić Slađana , Bell Pamela
Published: May 2018

An exposure to extreme trauma events leads to posttraumatic stress disorder (PTSD) in up to 14-50% of war survivors. Recent findings suggest that genetic factors could play a certain role in PTSD development. In order to illustrate this possibility, we present results of a pilot study on gender specific sample of Sarajevo civilians immediately after the war cessation. During the period 1992-1995, Sarajevo civilians experienced continuous life threatening events with a great risk of developing PTSD in such conditions.Our study included 100 women adjusted to same socio-demographic characteristics. All women were interviewed using Harvard Trauma Questionnaire (HTQ) and divided into two groups (domestic and returnees) according to exposure length to extreme war life events of six or forty-three months. Above 50% of total analysed sample fulfilled criteria for PTSD. Regarding duration in trauma exposure no significant difference between these two groups were found. The only significant predictor found was physical abuse (p>0.01) that still cannot explain why some women develop PTSD while others not.Several years after the war, PTSD frequencies are decreased and disorder became chronic and more severe. However, the PTSD prevalence remains high when compared to general population rates. Therefore, Sarajevo population being exposed for almost four years to extreme war life events represents unique model for comparative research on PTSD etiology within the light of latest findings in molecular genetics of PTSD.
Frequency of risk factors for cervical cancer among women in fertile age
Authors: Dubur Amela , Omanić Ajnija , Dubur Alen , Alispahić Sanja
Published: May 2018

Cervical and breast cancer are usually type of tumor that are found among women in fertile age in Bosnia and Herzegovina. Final goal was to establish frequency of risk factors that are responsible for development of those types of cancer as well as establish possibility of prevention, according to the existence of each risk factor. Research was conducted through out surveys among women which were selected by accident. The amount of questioned women is 200, and out of that number 70 (35%) were out of rural environment, 130 (65%) were from urban environment which led to statistic- processed information. Variables that were defining our interviews were: age, marital status, education level, stay during the war in B&H, number of given birth, consistency of gynecological examinations, changes that were found during the medical (gynecological) examination, number of sexual partners, usage of contraception, existence of sexual infections, usage of tobacco, existence of genetic factor. The most important fact is that over 50% of interviewees do not visit gynecologist, and that the gynecological infections are frequent. Usage of tobacco is in high percent founded among interviewees from urban environment (85%).
Foetal echocardiography
Authors: Dinarević Senka , Kumašin Lejla , Bilalović Vildan
Published: May 2018

A modern diagnostic and therapeutical approach to paediatric cardiology enables early application of foetal echocardiography in order of achieving diagnosis of congenital heart anomalies in utero. The aim of this study is to evaluate the precentage of prenatal diagnosis of congenital heart anomalies. This study has been conducted on 73 patients at Paediatric clinic of Clinical Centre of Sarajevo in a period from January 2000 until December 2004 with diagnosis of heart malformations. Among them 14 were preterm newborns, 40 boys. Diagnosis of cardiac anomalies with left to right shunt was done in 56.1%, obstructive 13.7%, cyanotic 1.36% and complex in 28.7% patients. The prenatal diagnosis was esatablished in 4 patients (5.5%) by ultrasound examination which is very low in comparison to other European countries. There is a need for making prenatal diagnosis of congenital heart anomalies in foetus as early as it can be done in order to treat cardiac anomalies in utero, to decraease the number of congenital heart anomalies and to reduce the cost of cardiosurgical and postsurgical treatment.
Serotesting of Human Brucellosis on Wider Area of Bosnia and Herzegovina
Authors: Hamzić Sadeta , Bešagić Edina , Zvizdić Šukrija , Aljičević Mufida , Bešlagić Omer , Puvačić Sandra
Published: May 2018

The study involved 286 individuals from different regions of Bosnia and Herzegovina, whose sera were tested in the Laboratory for specific diagnosis of human brucellosis in Microbiology Department of Medical Faculty of University in Sarajevo, during the period from 2000. to 2003. Sera were tested using Brucelloslide Test, qualitative agglutination test Rose Bengal. Using the agglutination test, we serologically confirmed a diagnosis of human brucellosis in 59 (20.62%) seropositive individuals, whereof 38 (64.40%) men and 21 (35.60%) women. Individuals with human brucellosis were the most present in the age group of 31-40 (22.03%) and 41-50 (22.03%). One serologically confirmed death case was registered. The most seropositive individuals were from Zenica-Doboj Canton (32.20%), Sarajevo Canton (28.82%), Herzegovina-Neretva Canton (23.73%), Central Bosnia Canton (13.55%) and Una-Sana Canton (1.70%). During our four-year study, it was serologically confirmed that human brucellosis is present in Bosnia and Herzegovina and, through seropositive testing, we revealed the level of general exposition to Brucella spp. on wider area of Bosnia and Herzegovina.
Oral Acute Toxicity of Polyenylphosphatidylcholine (PPC) in Rats
Authors: Krošnjar Sanja , Todić Maida , Bakić Sanela , Begović Begler , Zulić Irfan , Vehabović Midhat
Published: May 2018

Endogen phospholipids play a major role in determining the structure and nature of cell membranes. A deficiency of phospholipids in cellular membranes makes it almost impossible for the cell membrane to perform its function as a selective barrier between what passes in and out of the cell. Polyenylphosphatidylcholine chemical structure corresponds to that of endogen phospholipids, but it possesses functional superiority because of its content of unsaturated fatty acids. Polyenylphosphatidylcholine integrates in the cell membrane and organelle systems while becoming their constitutive elements. A healthy cell membrane leads to healthy cells and then healthy tissue and then to healthy organs or body systems and finally, healthy bodies and minds. For a long time, polyenylphosphatidylcholine in combination with vitamins has been used in the treatment of numerous health problems such as liver diseases, dyslipoproteinaemias and different intoxications with consequent liver failure. The main aim of toxicology studies is evaluation of the toxic potential and risks of human exposition to the substance. According to the Organization for Economic Cooperation and Development (OECD) acute oral toxicity refers to those adverse effects occurring following oraladministration of a single dose of a substance or multiple doses given within 24 hours. LD50 (median lethal dose), oral, is a statistically derived single dose of a substance that can be expected to cause death in 50 per cent of animals when administered by the oral route. Our acute toxicity study was performed on albino Wistar rats. Animals were randomised in three experimental and one control group, each of 5 males and 5 females. Study was based on the administration of a single oral dose of the test substance (polyenylphosphatidylcholine) to each experimental animal. There were three dose-levels of the test substance: 300, 500 and 1000 mg/kg. Test substance administration day was the first day of the observation period that lasted 14 days. Control animals were given milk vehicle. At the end of the study, no statistically significant differences between experimental and control animals were observed concerning the recorded parameters: body weight, respiratory rate, tremor, faeces and phonation quality, indicating the absence of the test substance acute toxicity.
Branching of Main Trunk of Left Coronary Artery and Importance of Her Diagonal Branch in Cases of Coronary Insuficiency
Authors: Lujinović Almira , Ovčina Fehim , Voljevica Alma , Hasanović Aida
Published: May 2018

Proficiency in the anatomy of coronary arteries and their variations is significant for proper interpretation of the coronary angiographies, assessment of the complexity and result of the coronary insufficiency as well as surgical myocardium revascularization.

The objective of this study is anatomy-radiology research of the methods of branching the main trunk of left coronary artery and to prove importance of the diagonal branch (ramus diagonalis) existence in the conditions of coronary insufficiency. In this study we have analyzed 100 coronary angiographies done at the Clinic for Heart Diseases and Rheumatism of the Clinic Center of University of Sarajevo and dissected 20 human hearts from the Institute of Anatomy. In our study we have come upon two methods of branching of main trunk of left coronary artery (bifurcation and trifurca-tion). By the method of the angiography we have found the bifurcation in 71% of cases while 65% of cases were proved by the dissection method. Trifurcation has been discovered in 29% of cases of analyzed angiographies i.e. 35% of cases of dissected hearts. We believe that third terminal branch of the left coronary artery should be marked as ramus diagonalis. This branch, including its anastomoses, presents important pattern of the collateral blood flow, which has special meaning, under conditions of coronary insufficiency.
Peripheral nerve blocks for perioperative management of patients having orthopedic surgery or trauma of the lower extremity
Authors: Iwata Takashige , Lakshman Sundaram , Singh Alpana , Yufa Marina , Claudio Rich , Hadžić Admir
Published: May 2018

Over the past decade several developments have lead to an increased interest in lower extremity PNBs including transient neurologic symptoms associated with spinal anesthesia, increased risk of epidural hematoma with the introduction of new antithromboembolic prophylaxis regimens, and evidence of improved rehabilitation outcome with continuous lower extremity PNBs. Simultaneously, the field of lower extremity blockade has been revolutionized through our better understanding of functional regional anesthesia anatomy, introduction of new drugs, better and more sophisticated equipment and wider teaching of lower extremity nerve block techniques. This review focuses on techniques and applications of lower extremity nerve blocks in patients having orthopedic surgery or trauma of the lower extremity, as well as potential complications and means to avoid them.
Our Experiences in Kidney Transplantation and Monitoring of Kidney Graft Outcomes
Authors: Rašić Senija , Džemidžić Jasminka , Aganović Kenana , Aganović Damir , Prcić Alden
Published: May 2018

Aim of kidney transplantation is to keep the functions of graft as long as possible, with an improvement of survival and quality of patients’ lives. Aim of this article was to show the outcome of kidney transplantation in patients who were treated and monitored on Institute of Nephrology, CCU of Sarajevo in period between 1996 and 2004. and to identify the factors which can interfere with graft surviving. Retrospective analysis of data from the register of kidney transplanted patients was done. In the mentioned period 29 kidney transplants were performed, and at the same time 15 previously transplanted patients (total of 44) were monitored. Patients were followed until death or graft insufficiency. Most often cause of chronic renal failure before the transplantation were glomerular diseases (3l,8% cases), and chronic pyelonephritis in 29,5% cases. Living-donor related kidney transplantation was performed in 56,8% of patients, living-donor unrelated in 27,3% of patients and cadaveric in 15,9% of patients. Post-transplant complications occurred in 29,5% of patients. Analysis of graft surviving on 12 months, 5 years and 10 years monitoring showed functional grafts in 87,5%, 80% and 75,0% of patients. Cumulative survival of patients on one year monitoring is 100%, on 5 year 100%, and on 10 year 93,8%. Primary causes of graft function loss were recurrent kidney diseases. Three patients (6,8%) died due to concomitant diseases, irrespective of the transplantation. Kidney transplantation is a successful treatment of a chronic renal failure with a high percentage of patients survival and long term graft survival, but also with serious post-transplant complications.
Embryogenesis of the rat telencephalon--a morphologic and stereologic analysis
Authors: Aličelebić Selma , Grbeša Đurđica
Published: May 2018

Comparative researches of borderline between telencephalon neuroepithelium and its surrounding mesenchyme in successive early developing stages lack in literature. The aim of this investigation was to carry out systematic morphologic and stereologic analyses of rattelencephalon in early developmental stages. We analysed semithin (1 microm) serial sections of rat embryonic brain from the 12th (E12) to the 15th (E15) day of gestation. The surface densities (SV) of an external mesenchymal surface, an internal mesenchymal surface and a neuroepithelial (ventricular) surface were examined stereologically and compared. The surface density of the external mesenchymal surface was the biggest at E12 (4,05 mm-1) and the least at E15 (1,87 mm-1)-p<0,0005. The surface density of the internal mesenchymal surface was the biggest at E12 (4,02 mm-1) and the least at E15 (2,69 mm-1)-p<0,0005. The surface density of the internal neuroepithelial surface was the biggest at E12 (3,31 mm-1) and the least at E15 (2,01 mm-1)-p<<0,0005. Our stereological examines give objective numerical proof of significant morphogenetic changes in telencephalon shape described by morphologicanalyses. The major advantage of stereological methods is the possibility to carry out the estimation procedures in specified, well-defined brain regions or layers.
Causal Factors of Acute Gastroenteritis in Infants and Young Children
Authors: Zvizdić Šukrija , Kapić Elvedina , Hamzić Sadeta
Published: May 2018

Respiratory, gastrointestinal and skin diseases represent the most common diseases in infants and young children. Causal factors of these diseases are important infectious agents and causes of pathological conditions in children, but they are also very important for their parents, as well as for people in their close environment. Greater incidence of infections in infants and young children can be explained in different ways. A cause can be insufficient maturity of their immune system, but also their exposure to infections within collective ac-comodations (cribs, nurseries, pre-school institutions), where they are, at the same time, exposed to a number of unknown agents. Today, a great emphasis is devoted to the ways and kinds of children’s nutrition. The problem of relation between infected young organism and infectious agent itself is also reflected in a long resistance and excretion of microorganisms in their exterior environment. It is well-known that microorganisms resist and excrete much longer in younger organisms, compared to adults, where their resistance and excretion is much shorter or very rare. Actually, adults have already formed protective immunity against particular infectious agents. It doesn’t prevent infections in adults, colonization of pathogènes, nor eventual development of disease. Established immunity can shorten the time necessary for excretion of microorganisms in their exterior environment and, if disease gets developed, it is of shorter duration and slower progress.
Nitric Oxide in Serum and Renal Tissue During Compensatory Renal Hypertrophy in Rats
Authors: Huskić Jasminko , Začiragić Asija , Babić Nermina , Mulabegović Nedžad
Published: May 2018

Nitric oxide (NO) level in serum and renal tissue has been examined in 15 male Wistar rats, body weight 200-250 g, 7 days after unilateral nephrectomy. All rats were ether-anaesthetized and the kidneys were removed by dorsolateral approach. NO concentration in serum and renal tissue was determined by classic colorimetric Griess reaction. Conversion of NO(3)(2-) into NO(2)(2-) was done with elementary zinc. Results have shown that NO concentration in renal tissue is statistically higher in rats 7 days after unilateral nephrectomy then in control renal tissue before compensatory kidney growth (p<0,02). There is no difference between NO concentration in serum before unilateral nephrectomy and 7 days after nephrectomy. These findings suggest that NO may play an important role in mediating the hemodynamic changes associated with reduced renal mass.
Effects of Hyperglycemia and Iron Deﬁciency on Kidney and Heart Function in Type 2 Diabetes Disease
Authors: Aščić-Buturović Belma , Heljić Bećir
Published: May 2018

Untreated anemia can caused significant cardiac and kidney damage. The aim of this study was to investigate the efficiency of anemia and hyperglycemia treatment in type 2 diabetes and their impact on kidney and heart impairment. The study is clinical retrospective and prospective and it was conducted in Clinic of Endocrinology, Diabetes Mellitus and Metabolic Diseases, University Clinical Center of Sarajevo. Prior to the study all patients were taking oral hypoglycemic drugs included sulfonylureas and biguanides. These subjects were put on 2 times daily fix mix insulin and biguanides after lunch. Each day, subjects received Iron tab 1 x 100 mg/ day, and C vitamin 1 x 100 mg/day. The results of our study are showing that effective treatment of glycaemia and anemia in patients with diabetes, reduces blood pressure, urine albumin secretion and pulse rate, diminishing cardiovascular damage and improving kidney function.
Correlation Between Eye Aperture Diameter and Complications in the Posterior Eye Segment after Nd-Yag Capsulotomy
Authors: Alimanović Halilović Emina
Published: May 2018

The aim of our research was to explore possible correlation between eye aperture diameter and occurrence of complications in the posterior eye segment after Nd-YAG capsulotomy. In the study, we analyzed 120 eyes of the patients who experienced opacities in the posterior capsule or developed secondary cataract after the surgery. All patients underwent Nd-YAG laser posterior capsulotomy. Thereafter, we monitored complications occurrence in the posterior eye segment in intervals of one hour, seven days, one month and six months following the surgery. The frequency of complications increased with time. Six months after Nd-YAG laser posterior capsulotomy we found changes in the posterior eye segment: hole in the anterior hyaloid membrane in case of 9 (7,50%) eyes, prolapse of the corpus vitrei in 2 (1,66%) cases, retinal hole in case of 5 (4,16%) eyes, macular hole in 3 (2,50%) eyes, retinal detachment in 3 (2,50%) eyes, cystoid macular edema in 2 (1,66%) eyes, (1,66%), and macular pack in case of 11 (9,16%) eyes. Aperture size in the posterior capsule directly correlates with the number of complications. We suggest that the aperture diameter should not exceed 4,0 mm.
Stress, Memory and Bosnian War Veterans
Authors: Sarač - Hadžihalilović Aida , Kulenović Amela , Kučukalić Abdulah
Published: May 2018

The aim of this study was to analyze cognitive dysfunction in PTSD. The testing included 79 Bosnian Army veterans, who participated in Bosnian war from 1992 to 1995. Out of 79 tested war veterans, 45 of developed PTSD while 34 did not. The veterans without PTSD where included in the control group. All the war veterans were of the same education level (secondary education) and between 30 and 50 years of age. Rivermead Behavioral Memory Test - RBMT was applied to all the subjects. The test was originally developed for the purpose of everyday memory problems identification. Clear goal of the 10 RBMT subtests is simulation of everyday life situations. PTSD group achieved significantly lower results than the control group. Results of the total score showed highly significant difference between PTSD and control group. Value of the t-test is t=10,056 with significance level of p<0,001. The same conclusion stands for any RBMT subtest. Numerous psychological studies on PTSD patients show more prominent psychological deficit in war veterans. Our study clearly confirms that finding.
Study of the Applicabilty of Content Uniformity and Dissolution Variation Test on Ropinirole Hydrochloride Tablets
Authors: Vranić Edina , Uzunović Alija
Published: May 2018

When considering single-dose preparations, it is fundamental that the patient receives in his individual dose an amount of drug close to that claimed on the label. Since drug content and content uniformity of single-dose preparations depend on a number of processes associated with their manufacture, it is obviously unrealistic to expect every unit of product to possess exactly the same amount of the active ingredient. For that reason, pharmacopeial standards and specifications have been established to provide limits for permissible variations in the amount of active ingredient of individual single-dose units. The aim of our study was to determine the applicability of content uniformity and dissolution variation test on ropinirole hydrochloride tablets. According to the results obtained, we may conclude that analyzed ropinirole hydrochloride tablets satisfied pharmacopeial requirements concerning content uniformity and dissolution testing. In this case RSD tended to increase with the decrease of the labeled strength. It is obvious from the R1 value, as well. On the other side, if consider larger number of lots, analyzed by different assay methods and various sample preparation procedures this correlation is less pronounced. This may be a consequence of different assay techniques applied, HPLC, UV-D1 or UV
Complementarity of Standard Cytogenetic Assays
Authors: Ibrulj Slavka , Haverić Sanin , Haverić Anja
Published: May 2018

Standard cytogenetic assays used in genotoxicology usually include chromosome aberrations analysis and micronucleus cytokinesis-block assay. Both tests originate on standard protocol for lymphocyte culture and can be used as complement or substitute to each other. Aim of this study was to evaluate complementarities between results of chromosome aberration analysis assay and results of micronucleus cytokinesis-block assay in representative sample of inhabitants from Bosnia and Herzegovina. The aim was achieved by calculating Pearson correlation coefficient and simple linear regression.