bluuebunny/crossref_metadata_2025 huggingface dataset:
Green Performance Evaluation System for Energy-Efficiency-Based Planning for Construction Site Layout
Authors: Wang Cynthia Changxin , Sepasgozar Samad M.E. , Wang Mudan , Sun Jun , Ning Xin
Published: December 2019

The location of temporary facilities in a construction project and the entire site layout plan directly affect project objectives such as time, labor cost, and material transportation and handling. The layout of construction sites also affects entrainment factors such as energy consumption, carbon footprints, and overall construction operation productivity. While site layout planning has been intensively investigated from a project objectives perspective, there have been very few studies of energy-efficiency-based planning, or of the sustainability performance of site layouts. This study developed a green performance evaluation system aimed at improving the sustainability of construction site layouts. The identified factors include six sustainable evaluation categories covering energy conservation and environmental protection, people-oriented principles, construction efficiency, intensity of economic growth, intensity of space use, and the overall control of process. An analytic hierarchy process (AHP) was adopted to determine the weight of each attribute and a fuzzy comprehensive evaluation method was established to carry out the evaluation. The 23 attributes adopted in this paper were identified in the literature; however, the major contribution of this paper is the development of a green performance evaluation system. This system integrates both qualitative and quantitative attributes and provides an overall evaluation of the environmental effectiveness of a construction site layout. The proposed evaluation system was validated with a commercial building project. The average utilization ratio of the case study site was calculated as 94%, and lessons learned are discussed in this paper. The case study analysis identified available site spaces around the building and examined how the arrangement of resources and facilities ensures effective connection between construction activities. The findings showed that the facility’s layout plays a crucial role in energy consumption and green performance. The proposed system will support construction project managers to create high-performance construction site layouts in more scientific and systematic ways.
Numerical Analysis of Heat Transfer Performance of In Situ Thermal Remediation of Large Polluted Soil Areas
Authors: Wang Weishu , Li Chuang , Li Yun-Ze , Yuan Man , Li Tong
Published: December 2019

In recent years soil contamination has become a global problem because of industrial development. In situ thermal remediation has been proposed recently to not only lower costs, but also reduce the environmental impact compared to other soil remediation technologies such as chemical remediation. During the soil thermal remediation process, factors such as soil type and water content affecting the heat transfer pose challenges. In this study, a simple mathematical model is presented and the heat transfer performance during the soil heating process is researched via COMSOL Multiphysics 5.3 software (COMSOL Inc., Stockholm, Sweden). The temperature distribution and heating period under different operating conditions are evaluated. The simulation results show that the average soil temperature exhibits three stages during the heating process. First, soil is heated from the ambient temperature to the water boiling temperature (100 °C). Then, the soil stays at the water boiling temperature for a while before reaching the target temperature. Simultaneously, the effects of initial water content and groundwater flow on heat transfer are also studied. In addition, the results of a simulation can provide a reference for in situ heating remediation technology.
Parametric Optimization and Thermodynamic Performance Comparison of Organic Trans-Critical Cycle, Steam Flash Cycle, and Steam Dual-Pressure Cycle for Waste Heat Recovery
Authors: Ren Liya , Wang Huaixin
Published: December 2019

Compared with the basic organic and steam Rankine cycles, the organic trans-critical cycle (OTC), steam flash cycle (SFC) and steam dual-pressure cycle (SDC) can be regarded as the improved cycle configurations for the waste heat power recovery since they can achieve better temperature matching between the heat source and working fluid in the heat addition process. This study investigates and compares the thermodynamic performance of the OTC, SFC, and SDC based on the waste heat source from the cement kiln with an initial temperature of 320 °C and mass flow rate of 86.2 kg/s. The effects of the main parameters on the cycle performance are analyzed and the parameter optimization is performed with net power output as the objective function. Results indicate that the maximum net power output of SDC is slightly higher than that of SFC and the OTC using n-pentane provides a 19.74% increase in net power output over the SDC since it can achieve the higher use of waste heat and higher turbine efficiency. However, the turbine inlet temperature of the OTC is limited by the thermal stability of the organic working fluid, hence the SDC outputs more power than that of the OTC when the initial temperature of the exhaust gas exceeds 415 °C.
Analysis of Archimedes Spiral Wind Turbine Performance by Simulation and Field Test
Authors: Jang Hyeonmu , Kim Dongmyeong , Hwang Yechan , Paek Insu , Kim Seungjoo , Baek Joonho
Published: December 2019

In this study, the performance of an Archimedes spiral wind turbine is analyzed by simulation and validated by a field test. It is characterized as a horizontal-axis drag-type wind turbine. This type of wind turbine cannot be analyzed by the well-known Blade Element Momentum(BEM) theory or Double Stream Tube Method(DSTM) commonly used to analyze the performance of lift-type wind turbines. Therefore, the computational fluid dynamics (CFD) method was applied. From the simulation, the power coefficient, known as the mechanical efficiency of the rotor, the tip speed ratio was obtained. The maximum power coefficient, and the corresponding tip speed ratio were found to be 0.293 and 2.19, respectively. In addition, the electrical efficiency with respect to the rotational speed of the generator was obtained through generator–controller test. The obtained mechanical and electrical efficiencies were used to predict the power curve of the wind turbine. Finally, the predicted performance of the wind turbine, including the electrical losses, was validated by the field test. The maximum error between the prediction and the measured power was found to be less than 7.80%.
Application of the Climograph for the Greenhouse Plan of Subtropical and Tropical Regions
Authors: Chen Chiachung
Published: December 2019

The technological levels of greenhouse influence significantly the yields of the crop production and increase the investment cost. The balance between the development of the technological level and the achieving of the increased crop production in the controlled environment is always the primary concern. In this study, the climograph was obtained by plotting the mean minimum monthly temperature versus mean maximum monthly temperature. This climagraph was combined at the optimum temperature ranges of three fruit vegetables and a simple microclimate model was introduced to evaluate the controlling performance of internal air temperature in greenhouses. In subtropical areas, seven locations were selected in order to demonstrate the application of the climograph for the cultivation of species. The rotation of temperate and thermophile plant in Yulin, Taiwan was proposed. Melon, as the thermophile crop, was the only crop that could be cultivated in greenhouses in the two locations of low land areas. The results in three locations of high land areas indicated that all temperate vegetables were suited well to be cultivated in these areas. Thus, the significant highlight of this method was that it could also be successfully used for other species at different locations for the greenhouse production to reduce the energy consumption.
Modelling and Design of a Low-Level Turn-to-Turn Fault Protection Scheme for Extra-High Voltage Magnetically Controlled Shunt Reactor
Authors: Khan M. Asghar , Zheng Tao
Published: December 2019

The objective of this paper is to model and design a low-level turn-to-turn fault (T2TF) protection scheme for a magnetically controlled shunt reactor (MCSR), during incipient stage under 10% to 100% operating capacity. Due to the structural and functional differences of all the three windings in extra-high voltage (EHV) MCSR, a separate mechanism of detecting a T2TF in each winding is necessary. For this purpose, a detailed mathematical and structural analysis of the model is performed, and a comprehensive protection scheme based on the internal changes in magnetic and electric parameters of the windings is formulated to detect 3% T2TF in power windings (PWs), control windings (CtrWs), compensation windings (CpWs), and to differentiate it from other abnormalities. The main idea of the scheme is to perform the currents magnitude comparison of respective winding with the predefined settings values and decide necessary action. The proposed scheme is also capable of identifying the faulty winding along with faulty phase. The scheme is tested under different operating capacities (10%, 50%, 100%), and other types of unusual conditions, i.e., direct energization, pre-excited energization, power regulation, internal and external faults. The results demonstrate the effectiveness of the proposed scheme. The work of this paper is applicable in the areas of power system transmission and power system protection. The simulations are carried out on MATLAB/Simulink-based models.
Effects of Plum Plantation Ages on Soil Organic Carbon Mineralization in the Karst Rocky Desertification Ecosystem of Southwest China
Authors: Yang Hui , Mo Biqin , Zhou Mengxia , Zhu Tongbin , Cao Jianhua
Published: December 2019

Soil organic carbon (SOC) mineralization is closely related to carbon source or sink of terrestrial ecosystem. Understanding SOC mineralization under plum plantation is essential for improving our understanding of SOC responses to land-use change in karst rocky desertification ecosystem. In this study, 2-year, 5-year, and 20-year plum plantations and adjacent abandoned land dominated by herbs were sampled, and a 90-day incubation experiment was conducted to investigate the effect of plum plantations with different ages on SOC mineralization in subtropical China. Results showed that: (1) Plum plantation significantly decreased SOC content compared with abandoned land, but there was no significant difference in SOC content among plum plantations with different ages. Oppositely, the accumulative SOC mineralization (Ct) and potential SOC mineralization (C0) showed different responses to plum plantation ages. (2) The dynamics of the SOC mineralization were a good fit to a first-order kinetic model. Both C0 and Ct in calcareous soil of this study was several- to 10-folds lower than other soils in non-karst regions, indicating that SOC in karst regions has higher stability. (3) Correlation analysis revealed that both Ct and C0 was significantly correlated with soil calcium (Ca), suggesting an important role of Ca in SOC mineralization in karst rocky desertification areas. In conclusion, a Ca-rich geological background controls SOC mineralization in karst rocky desertification areas.
The Biological Durability of Thermally- and Chemically-Modified Black Pine and Poplar Wood Against Basidiomycetes and Mold Action
Authors: Kamperidou Vasiliki
Published: December 2019

Wood of black pine and poplar species were subjected to thermal modification under variant conditions, while subsequently, a number of the thermally-modified black pine specimens were subjected to surface modification with organosilane solutions, and the biological resistances of the different materials were examined using laboratory agar block tests against the action of basidiomycetes and microfungi. Thermally-modified pine specimens were exposed to the brown rot fungi Coniophora puteana and Oligoporus placenta, whereas poplar wood was exposed to the white rot fungus Trametes versicolor and O. placenta. Regarding the biological durability of thermally-chemically-treated pine wood with organosilanes, it was tested against the action of C. puteana. Additionally, both of the thermally-treated wood species, as well as thermally-chemically-treated pine wood were exposed to a microfungi mixture, so that the wood treatments efficacy would be evaluated through a visual assessment of fungal growth on the specimen’s surface The thermal treatments seem to increase the biological resistance of black pine against C. puteana by 9.65–36.73% compared to unmodified wood. The most significant increase in biological durability among all the thermally-treated wood categories was recorded by O. placenta, with 28.75–68.46% lower mass losses in treated pine specimens and 31.98–64.72% in thermally-treated poplar, respectively, compared to unmodified wood. The resistance of treated poplar against T. versicolor was also found increased (13.25–46.08%), compared to control. Thermal modification affected positively the biological resistance of both species, though it did not manage to protect effectively pine and poplar wood from the microfungi action. The combination of thermal and organosilanes treatment revealed a significant improvement of the durability of pine wood compared to? control (45.68–87.83% lower mass losses against C. puteana), as well as against the microfungi action, with the presence of benzin to have a positive effect on the silanes solutions performance and protective action.
Associations of Heat-Shock Protein Expression with Meat Quality and Sensory Quality Characteristics in Highly Marbled Longissimus Thoracis Muscle from Hanwoo Steers Categorized by Warner–Bratzler Shear Force Value
Authors: Oh Eunmi , Lee Boin , Choi Young Min
Published: December 2019

The influence of heat-shock protein (HSP) concentrations at 45 min and 24 h postmortem on meat quality and sensory quality characteristics of longissimus thoracis muscle from highly marbled Hanwoo steers (beef marbling standard grade 6–8) was investigated. Muscle samples were segregated into three groups based on Warner–Bratzler shear force (WBSF) value. The low group exhibited a lower WBSF value compared to the medium and high groups (37.8 vs. 48.9 and 64.3 N, p < 0.001). Muscle pH at 45 min and 24 h postmortem was not different (p > 0.05), and all groups exhibited low ultimate pH value (pH < 5.8). Beef steaks from the low group were significantly easier to pierce and chew, and they left less perceptible residue than the high group (p < 0.05). These differences in tenderness attributes were associated with differences in small HSPs at 45 min postmortem, with the low group exhibiting a lower level of αβ-crystallin and higher levels of HSP20 and HSP27 compared with the high group (p < 0.05). No differences were observed for small HSPs, HSP70, and HSP90 at 24 h postmortem (p > 0.05). Therefore, the expression levels of small HSPs at 45 min postmortem seems to have the potential to be an indicator of tenderness in highly marbled Hanwoo beef with low ultimate pH.
Efficacy of Commercial Sanitizers Used in Food Processing Facilities for Inactivation of Listeria monocytogenes, E. Coli O157:H7, and Salmonella Biofilms
Authors: Aryal Manish , Muriana Peter M.
Published: December 2019

Bacteria entrapped in biofilms are a source of recurring problems in food processing environments. We recently developed a robust, 7-day biofilm microplate protocol for creating biofilms with strongly adherent strains of Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella serovars that could be used to examine the effectiveness of various commercial sanitizers. Listeria monocytogenes 99-38, E.coli O157:H7 F4546, and Salmonella Montevideo FSIS 051 were determined from prior studies to be good biofilm formers and could be recovered and enumerated from biofilms following treatment with trypsin. Extended biofilms were generated by cycles of growth and washing daily, for 7 days, to remove planktonic cells. We examined five different sanitizers (three used at two different concentrations) for efficacy against the three pathogenic biofilms. Quaternary ammonium chloride (QAC) and chlorine-based sanitizers were the least effective, showing partial inhibition of the various biofilms within 2 h (1–2 log reduction). The best performing sanitizer across all three pathogens was a combination of modified QAC, hydrogen peroxide, and diacetin which resulted in ~6–7 log reduction, reaching levels below our limit of detection (LOD) within 1–2.5 min. All treatments were performed in triplicate replication and analyzed by one way repeated measures analysis of variance (RM-ANOVA) to determine significant differences (p < 0.05) in the response to sanitizer treatment over time. Analysis of 7-day biofilms by scanning electron microscopy (SEM) suggests the involvement of extracellular polysaccharides with Salmonella and E. coli, which may make their biofilms more impervious to sanitizers than L. monocytogenes.
Beverage and Food Fragrance Biotechnology, Novel Applications, Sensory and Sensor Techniques: An Overview
Authors: Vilela Alice , Bacelar Eunice , Pinto Teresa , Anjos Rosário , Correia Elisete , Gonçalves Berta , Cosme Fernanda
Published: December 2019

Flavours and fragrances are especially important for the beverage and food industries. Biosynthesis or extraction are the two main ways to obtain these important compounds that have many different chemical structures. Consequently, the search for new compounds is challenging for academic and industrial investigation. This overview aims to present the current state of art of beverage fragrance biotechnology, including recent advances in sensory and sensor methodologies and statistical techniques for data analysis. An overview of all the recent findings in beverage and food fragrance biotechnology, including those obtained from natural sources by extraction processes (natural plants as an important source of flavours) or using enzymatic precursor (hydrolytic enzymes), and those obtained by de novo synthesis (microorganisms’ respiration/fermentation of simple substrates such as glucose and sucrose), are reviewed. Recent advances have been made in what concerns “beverage fragrances construction” as also in their application products. Moreover, novel sensory and sensor methodologies, primarily used for fragrances quality evaluation, have been developed, as have statistical techniques for sensory and sensors data treatments, allowing a rapid and objective analysis.
The Chalcone Isomerase Family in Cotton: Whole-Genome Bioinformatic and Expression Analyses of the Gossypium barbadense L. Response to Fusarium Wilt Infection
Authors: Zu Qian-Li , Qu Yan-Ying , Ni Zhi-Yong , Zheng Kai , Chen Qin , Chen Quan-Jia
Published: December 2019

Chalcone isomerase (CHI) is a key component of phenylalanine metabolism that can produce a variety of flavonoids. However, little information and no systematic analysis of CHI genes is available for cotton. Here, we identified 33 CHI genes in the complete genome sequences of four cotton species (Gossypium arboretum L., Gossypium raimondii L., Gossypium hirsutum L., and Gossypium barbadense L.). Cotton CHI proteins were classified into two main groups, and whole-genome/segmental and dispersed duplication events were important in CHI gene family expansion. qRT-PCR and semiquantitative RT-PCR results suggest that CHI genes exhibit temporal and spatial variation and respond to infection with Fusarium wilt race 7. A preliminary model of CHI gene involvement in cotton evolution was established. Pairwise comparison revealed that seven CHI genes showed higher expression in cultivar 06-146 than in cultivar Xinhai 14. Overall, this whole-genome identification unlocks a new approach to the comprehensive functional analysis of the CHI gene family, which may be involved in adaptation to plant pathogen stress.
Sex Determination Using RNA-Sequencing Analyses in Early Prenatal Pig Development
Authors: Teixeira Susana A. , Ibelli Adriana M. G. , Cantão Maurício E. , Oliveira Haniel C. de , Ledur Mônica C. , Peixoto Jane de O. , Marques Daniele B. D. , Costa Karine A. , Coutinho Luiz. L. , Guimarães Simone E. F.
Published: December 2019

Sexual dimorphism is a relevant factor in animal science, since it can affect the gene expression of economically important traits. Eventually, the interest in the prenatal phase in a transcriptome study may not comprise the period of development in which male and female conceptuses are phenotypically divergent. Therefore, it would be interesting if sex differentiation could be performed using transcriptome data, with no need for extra techniques. In this study, the sex of pig conceptuses (embryos at 25 days-old and fetuses at 35 days-old) was determined by reads counts per million (CPM) of Y chromosome-linked genes that were discrepant among samples. Thus, ten genes were used: DDX3Y, KDM5D, ZFY, EIF2S3Y, EIF1AY, LOC110255320, LOC110257894, LOC396706, LOC100625207, and LOC110255257. Conceptuses that presented reads CPM sum for these genes (ΣCPMchrY) greater than 400 were classified as males and those with ΣCPMchrY below 2 were classified as females. It was demonstrated that the sex identification can be performed at early stages of pig development from RNA-sequencing analysis of genes mapped on Y chromosome. Additionally, these results reinforce that sex determination is a mechanism conserved across mammals, highlighting the importance of using pigs as an animal model to study sex determination during human prenatal development.
Existing and Emerging Metabolomic Tools for ALS Research
Authors: Germeys Christine , Vandoorne Tijs , Bercier Valérie , Van Den Bosch Ludo
Published: December 2019

Growing evidence suggests that aberrant energy metabolism could play an important role in the pathogenesis of amyotrophic lateral sclerosis (ALS). Despite this, studies applying advanced technologies to investigate energy metabolism in ALS remain scarce. The rapidly growing field of metabolomics offers exciting new possibilities for ALS research. Here, we review existing and emerging metabolomic tools that could be used to further investigate the role of metabolism in ALS. A better understanding of the metabolic state of motor neurons and their surrounding cells could hopefully result in novel therapeutic strategies.
CaMad2 Promotes Multiple Aspects of Genome Stability Beyond Its Direct Function in Chromosome Segregation
Authors: Vossen Maicy L. , Alhosawi Hanaa M. , Aney Katherine J. , Burrack Laura S.
Published: December 2019

Mad2 is a central component of the spindle assembly checkpoint required for accurate chromosome segregation. Additionally, in some organisms, Mad2 has roles in preventing mutations and recombination through the DNA damage response. In the fungal pathogen Candida albicans, CaMad2 has previously been shown to be required for accurate chromosome segregation, survival in high levels of hydrogen peroxide, and virulence in a mouse model of infection. In this work, we showed that CaMad2 promotes genome stability through its well-characterized role in promoting accurate chromosome segregation and through reducing smaller scale chromosome changes due to recombination and DNA damage repair. Deletion of MAD2 decreased cell growth, increased marker loss rates, increased sensitivity to microtubule-destabilizing drugs, and increased sensitivity to DNA damage inducing treatments. CaMad2-GFP localized to dots, consistent with a role in kinetochore binding, and to the nuclear periphery, consistent with an additional role in DNA damage. Furthermore, deletion of MAD2 increases growth on fluconazole, and fluconazole treatment elevates whole chromosome loss rates in the mad2∆/∆ strain, suggesting that CaMad2 may be important for preventing fluconazole resistance via aneuploidy.
Absence of W Chromosome in Psychidae Moths and Implications for the Theory of Sex Chromosome Evolution in Lepidoptera
Authors: Hejníčková Martina , Koutecký Petr , Potocký Pavel , Provazníková Irena , Voleníková Anna , Dalíková Martina , Visser Sander , Marec František , Zrzavá Magda
Published: December 2019

Moths and butterflies (Lepidoptera) are the largest group with heterogametic females. Although the ancestral sex chromosome system is probably Z0/ZZ, most lepidopteran species have the W chromosome. When and how the W chromosome arose remains elusive. Existing hypotheses place the W origin either at the common ancestor of Ditrysia and Tischeriidae, or prefer independent origins of W chromosomes in these two groups. Due to their phylogenetic position at the base of Ditrysia, bagworms (Psychidae) play an important role in investigating the W chromosome origin. Therefore, we examined the W chromosome status in three Psychidae species, namely Proutia betulina, Taleporia tubulosa, and Diplodoma laichartingella, using both classical and molecular cytogenetic methods such as sex chromatin assay, comparative genomic hybridization (CGH), and male vs. female genome size comparison by flow cytometry. In females of all three species, no sex chromatin was found, no female-specific chromosome regions were revealed by CGH, and a Z-chromosome univalent was observed in pachytene oocytes. In addition, the genome size of females was significantly smaller than males. Overall, our study provides strong evidence for the absence of the W chromosome in Psychidae, thus supporting the hypothesis of two independent W chromosome origins in Tischeriidae and in advanced Ditrysia.
Persuasion without Words: Confucian Persuasion and the Supernatural
Authors: Yi Se-Hyoung
Published: December 2019

This article revisits the nonverbal rhetorical tradition in Confucianism and examines how Confucianism actualized the tradition through its careful consideration of supernatural forces. In Confucianism, genuine persuasion produces actual change and transformation of one’s course of action, not merely verbal conviction. Speech only is not enough to genuinely persuade others. A speaker must transform others by his exemplary acts in the rites and holy ceremonies where supernatural forces and the notion of the afterlife hold a significant place. While Confucius was not interested in discussing the existence of demons and ghosts or their actual function in society, he recognized that their supposed and assumed existence in holy rites would provide society with an opportunity for genuine persuasion, which leads people to actual changes and reforms in their political and moral life. Discussing the nonverbal mode of persuasion in Confucianism may enhance contemporary democracy in two aspects. First, nonverbal persuasion recognizes those who may have difficulty in actively participating in verbal communication, such as the disabled, immigrants, foreigners, and politically and socially marginalized people, in political discourses. Second, the positive role of civic religion in contemporary societies may be discovered.
Differential Diagnosis of Uterine Leiomyoma and Uterine Sarcoma Using Magnetic Resonance Images: A Literature Review
Authors: Suzuki Ayako , Aoki Masato , Miyagawa Chiho , Murakami Kosuke , Takaya Hisamitsu , Kotani Yasushi , Nakai Hidekatsu , Matsumura Noriomi
Published: December 2019

MRI plays an essential role in patients before treatment for uterine mesenchymal malignancies. Although MRI includes methods such as diffusion-weighted imaging and dynamic contrast-enhanced MRI, the differentiation between uterine myoma and sarcoma always becomes problematic. The present paper discusses important findings to ensure that sarcomas are not overlooked in magnetic resonance (MR) images, and we describe the update in the differentiation between uterine leiomyoma and sarcoma with recent reports.
Characteristics of Bacterial Community and Function in Paddy Soil Profile around Antimony Mine and Its Response to Antimony and Arsenic Contamination
Authors: Huang Bocong , Long Jian , Liao Hongkai , Liu Lingfei , Li Juan , Zhang Jumei , Li Yirong , Wang Xian , Yang Rui
Published: December 2019

Research of bacterial communities and metabolism potential of paddy soils contaminated by antimony (Sb) and arsenic (As) are vital to acquire understanding for their bioremediation. Here, the relative abundance of Sb and As metabolism genes, the diversity and composition of the bacterial community, and the influences of geochemical properties and the bacterial community and metabolism potential have been researched by Tax4Fun2 prediction and high-throughput sequencing. LEfSe (linear discriminant analysis effect size) analysis shown different taxa were enriched in dissimilar soil layers. RDA (Redundancy analysis) and relative importance analysis indicated the main properties including total sulfur (TS), total organic carbon (TOC), pH, and the bioavailable fractions of Sb and As affects the bacterial community, which Sbrec, Astot, and Asrec had greater impact on the bacterial taxonomic community. For example, Asrec, Astot, and Sbrec had a positive correlation with Chloroflexi and Rokubacteria, but negatively correlated with Proteobacteria and Actinobacteria. Obtaining metabolic function genes by using the tax prediction method. RDA, relative importance analysis, and co-occurrence network analysis showed the geochemical properties and bacterial community affected Sb and As related bacterial functions. The partial least squares path model (PLS-PM) analysis indicated Sb and As contamination fractions had negative effects on ecological function, bacterial community structure had positive influences on ecological function, and the direct effects of geochemical properties on ecological function was greater than community structure. The direct impact of As contamination fractions on bacterial community structure was greater than Sb, while the direct impact of Sb contamination fractions on bacterial function was more remarkable than As. Obviously, this study provides a scientific basis for the potential of biochemical remediation of Sb and As contamination in paddy soils profile.
Atrial Fibrillation in Athletes—Features of Development, Current Approaches to the Treatment, and Prevention of Complications
Authors: Achkasov Evgeny , Bondarev Sergey , Smirnov Victor , Waśkiewicz Zbigniew , Rosemann Thomas , Nikolaidis Pantelis Theodoros , Knechtle Beat
Published: December 2019

Atrial fibrillation (AF) is one of the most common types of cardiac arrhythmias. This review article highlights the problem of the development of atrial fibrillation in individuals engaged in physical activity and sports. Predisposing factors, causes, and development mechanisms of atrial fibrillation in athletes from the perspective of the authors are described. Methods of treatment, as well as prevention of thromboembolic complications, are discussed. Directions for further studies of this problem and prevention of complications are proposed.
Cracks Reinforce the Interactions among Soil Bacterial Communities in the Coal Mining Area of Loess Plateau, China
Authors: Luo Zhanbin , Ma Jing , Chen Fu , Li Xiaoxiao , Hou Huping , Zhang Shaoliang
Published: December 2019

Soil microorganisms play a key role in global biogeochemical changes. To understand the interactions among soil bacterial communities and their responses to extreme environments, the soil properties and bacterial community diversity were determined in the post-mining ecosystem of the Loess Plateau, China. The results showed that the soil temperature, pH, organic matter, available phosphorus, and available potassium values were significantly reduced in the post-mining cracks area. However, the richness and uniformity of soil bacterial communities increased by about 50% in the post-mining cracks area. Soil microbial community structure and the network interactions tended to be complex and strengthened in the post-mining cracks area. Moreover, soil nutrient loss caused the differences in soil bacterial community structure compositions in the post-mining cracks area. Furthermore, the relationships between soil physicochemical properties and different modules of the soil bacterial molecular ecological network were changed in a complex manner in the post-mining cracks area. This study provides a theoretical basis for adaptive management and response to cracks in post-mining areas and under other extreme conditions.
The Relationship between the Number of Daily Health-Related Behavioral Risk Factors and Sleep Health of the Elderly in China
Authors: Liu Xiaojun , Chen Jingshu , Zhou Jiayi , Liu Jianjian , Lertpitakpong Chanida , Tan Anran , Wu Shaotang , Mao Zongfu
Published: December 2019

This study examined the cross-sectional association among a number of daily health-related behavioral risk factors and sleep among Chinese elderly. A sample of 4993 adults, aged 60 years and older, from the China’s Health-Related Quality of Life Survey for Older Adults 2018 was included in this study. Five daily health-related behaviors, which included smoking, drinking, unhealthy eating habits, insufficient leisure activities, and physical inactivity were measured. Sleep disturbances and sleep quality were used to represent the respondents’ sleep status. Multiple logistic regression models and multiple linear regression models were established. The odds ratios (ORs) of sleep disturbances for those with one to five health-related risk behaviors were 1.41 (95% CI = 1.11 to 1.78), 2.09 (95% CI = 1.66 to 2.63), 2.54 (95% CI = 1.99 to 3.25), 2.12 (95% CI = 1.60 to 2.80), and 2.49 (95% CI = 1.70 to 3.65), respectively. Individuals with one health-related risk behavior (B = 0.14, 95% CI = −0.23 to −0.06), two health-related risk behaviors (B = 0.21, 95% CI = −0.30 to −0.13), three health-related risk behaviors (B = 0.46, 95% CI = −0.55 to −0.37), four health-related risk behaviors (B = 0.50, 95% CI = −0.62 to −0.39), and five health-related risk behaviors (B = 0.83, 95% CI = −1.00 to −0.66) showed lower scores of self-perceived sleep quality. Having multiple health-risk behaviors was positively correlated with a higher risk of sleep disturbances among Chinese elderly. Moreover, elderly individuals with multiple health-related risk behaviors were significantly associated with poorer sleep quality.
Recognizing the Effect of Ecosystem Disruption on Human Health and Neurodevelopment
Authors: Ming Xue , Ray Carly
Published: December 2019

The topics of climate change and ecosystem disruption are at the forefront of global discussion. Accordingly, there is a sense of urgency among citizens, environmental experts, and political leaders for action and policy change. While the effect of a changing climate on the environment is well recognized, its impact on the human body has not been sufficiently described. In our review, we will attempt to outline some of these effects. We will discuss how the recent surge of immunologic disease may be related to the changing profile of microorganisms and antigens in our every-day environment. We will explore how the introduction of antibiotics may result in an altered gut microbiome, and subsequently abnormal neurodevelopment. Finally, we will discuss a possible link between chemical preservatives and neoplastic disease.
Characterization and Arsenic Adsorption Behaviors of Water Treatment Residuals from Waterworks for Iron and Manganese Removal
Authors: Zeng Huiping , Qiao Tongda , Zhao Yunxin , Yu Yaping , Zhang Jie , Li Dong
Published: December 2019

Water treatment residuals (WTRs), obtained from a groundwater treatment plant for biological iron and manganese removal, were investigated and used as adsorbents for arsenic removal. The surface morphology and structural features of the WTRs were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and Brunauner–Emmett–Teller analysis (BET). Laboratory experiments were also carried out to test the adsorption capability and adaptability of WTRs on both As (III) and As (V) removal from the water. The results showed that the WTRs were mainly amorphous and had a large specific surface area of 253.152 m2/g. The maximum adsorption capacities, evaluated using the Langmuir isotherm equation, were 36.53 mg/g and 40.37 mg/g for As (III) and As (V), respectively. The pseudo-second-order model fitted the kinetic data better, with R2 more than 0.99 for both As (III) and As (V). The removal of As (V) decreased with the increase in pH, especially when the pH was above 9, whereas for As (III), the removal effectiveness almost remained constant at both acidic and neutral pHs. H2PO4− and SiO32− could strongly inhibit arsenic adsorption onto the WTRs, and the effect of other ions was little.
Non-Employment Activity Type Imputation from Points of Interest and Mobility Data at an Individual Level: How Accurate Can We Get?
Authors: Bantis Thanos , Haworth James
Published: December 2019

Human activity type inference has long been the focus for applications ranging from managing transportation demand to monitoring changes in land use patterns. Today’s ever increasing volume of mobility data allow researchers to explore a wide range of methodological approaches for this task. Such data, however, lack reference observations that would allow the validation of methodological approaches. This research proposes a methodological framework for urban activity type inference using a Dirichlet multinomial dynamic Bayesian network with an empirical Bayes prior that can be applied to mobility data of low spatiotemporal resolution. The method was validated using open source Foursquare data under different isochrone configurations. The results provide evidence of the limits of activity detection accuracy using such data as determined by the Area Under Receiving Operating Curve (AUROC), log-loss, and accuracy metrics. At the same time, results demonstrate that a hierarchical modeling framework can provide some flexibility against the challenges related to the nature of unsupervised activity classification using trajectory variables and POIs as input.
Critical Assessment of Streptomyces spp. Able to Control Toxigenic Fusaria in Cereals: A Literature and Patent Review
Authors: Colombo Elena Maria , Kunova Andrea , Cortesi Paolo , Saracchi Marco , Pasquali Matias
Published: December 2019

Mycotoxins produced by Fusarium species on cereals represent a major concern for food safety worldwide. Fusarium toxins that are currently under regulation for their content in food include trichothecenes, fumonisins, and zearalenone. Biological control of Fusarium spp. has been widely explored with the aim of limiting disease occurrence, but few efforts have focused so far on limiting toxin accumulation in grains. The bacterial genus Streptomyces is responsible for the production of numerous drug molecules and represents a huge resource for the discovery of new molecules. Streptomyces spp. are also efficient plant colonizers and able to employ different mechanisms of control against toxigenic fungi on cereals. This review describes the outcomes of research using Streptomyces strains and/or their derived molecules to limit toxin production and/or contamination of Fusarium species in cereals. Both the scientific and patent literature were analyzed, starting from the year 2000, and we highlight promising results as well as the current pitfalls and limitations of this approach.
Natural Flavonol, Myricetin, Enhances the Function and Survival of Cryopreserved Hepatocytes In Vitro and In Vivo
Authors: Cui Changhao , Enosawa Shin , Matsunari Hitomi , Nagashima Hiroshi , Umezawa Akihiro
Published: December 2019

To improve the therapeutic potential of hepatocyte transplantation, the effects of the mitogen-activated protein kinase kinase 4 (MKK4) inhibitor, myricetin (3,3′,4′,5,5′,7-hexahydroxylflavone) were examined using porcine and human hepatocytes in vitro and in vivo. Hepatocytes were cultured, showing the typical morphology of hepatic parenchymal cell under 1–10 µmol/L of myricetin, keeping hepatocyte specific gene expression, and ammonia removal activity. After injecting the hepatocytes into neonatal Severe combined immunodeficiency (SCID) mouse livers, cell colony formation was found at 10–15 weeks after transplantation. The human albumin levels in the sera of engrafted mice were significantly higher in the recipients of myricetin-treated cells than non-treated cells, corresponding to the size of the colonies. In terms of therapeutic efficacy, the injection of myricetin-treated hepatocytes significantly prolonged the survival of ornithine transcarbamylase-deficient SCID mice from 32 days (non-transplant control) to 54 days. Biochemically, the phosphorylation of MKK4 was inhibited in the myricetin-treated hepatocytes. These findings suggest that myricetin has a potentially therapeutic benefit that regulates hepatocyte function and survival, thereby treating liver failure.
Annexin A2 Deficiency Exacerbates Neuroinflammation and Long-Term Neurological Deficits after Traumatic Brain Injury in Mice
Authors: Liu Ning , Jiang Yinghua , Chung Joon Yong , Li Yadan , Yu Zhanyang , Kim Jeong Woo , Lok Josephine M. , Whalen Michael J. , Wang Xiaoying
Published: December 2019

Our laboratory and others previously showed that Annexin A2 knockout (A2KO) mice had impaired blood–brain barrier (BBB) development and elevated pro-inflammatory response in macrophages, implying that Annexin A2 (AnxA2) might be one of the key endogenous factors for maintaining homeostasis of the neurovascular unit in the brain. Traumatic brain injury (TBI) is an important cause of disability and mortality worldwide, and neurovascular inflammation plays an important role in the TBI pathophysiology. In the present study, we aimed to test the hypothesis that A2KO promotes pro-inflammatory response in the brain and worsens neurobehavioral outcomes after TBI. TBI was conducted by a controlled cortical impact (CCI) device in mice. Our experimental results showed AnxA2 expression was significantly up-regulated in response to TBI at day three post-TBI. We also found more production of pro-inflammatory cytokines in the A2KO mouse brain, while there was a significant increase of inflammatory adhesion molecules mRNA expression in isolated cerebral micro-vessels of A2KO mice compared with wild-type (WT) mice. Consistently, the A2KO mice brains had a significant increase in leukocyte brain infiltration at two days after TBI. Importantly, A2KO mice had significantly worse sensorimotor and cognitive function deficits up to 28 days after TBI and significantly larger brain tissue loss. Therefore, these results suggested that AnxA2 deficiency results in exacerbated early neurovascular pro-inflammation, which leads to a worse long-term neurologic outcome after TBI.
Lab-On-A-Chip for the Development of Pro-/Anti-Angiogenic Nanomedicines to Treat Brain Diseases
Authors: Subramaniyan Parimalam Subhathirai , Badilescu Simona , Sonenberg Nahum , Bhat Rama , Packirisamy Muthukumaran
Published: December 2019

There is a huge demand for pro-/anti-angiogenic nanomedicines to treat conditions such as ischemic strokes, brain tumors, and neurodegenerative diseases such as Alzheimer’s and Parkinson’s. Nanomedicines are therapeutic particles in the size range of 10–1000 nm, where the drug is encapsulated into nano-capsules or adsorbed onto nano-scaffolds. They have good blood–brain barrier permeability, stability and shelf life, and able to rapidly target different sites in the brain. However, the relationship between the nanomedicines’ physical and chemical properties and its ability to travel across the brain remains incompletely understood. The main challenge is the lack of a reliable drug testing model for brain angiogenesis. Recently, microfluidic platforms (known as “lab-on-a-chip” or LOCs) have been developed to mimic the brain micro-vasculature related events, such as vasculogenesis, angiogenesis, inflammation, etc. The LOCs are able to closely replicate the dynamic conditions of the human brain and could be reliable platforms for drug screening applications. There are still many technical difficulties in establishing uniform and reproducible conditions, mainly due to the extreme complexity of the human brain. In this paper, we review the prospective of LOCs in the development of nanomedicines for brain angiogenesis–related conditions.
Comparative Transcriptome Analysis between a Novel Allohexaploid Cotton Progeny CMS Line LD6A and Its Maintainer Line LD6B
Authors: Zheng Jie , Kong Xiangjun , Li Bin , Khan Aziz , Li Zhiling , Liu Yiding , Kang Haodong , Ullah Dawar Farman , Zhou Ruiyang
Published: December 2019

Cytoplasmic male sterility (CMS) is an important agronomic feature and provides an effective tool for heterosis utilization of crops. This study reports the comparative transcriptomic sketches between a novel allohexaploid cotton progeny CMS line LD6A and its maintainer line LD6B using de novo transcriptome sequencing technology at the pollen abortion stage. A total of 128,901 Unigenes were identified, in which 2007 were upregulated and 11,864 were downregulated. The significantly differentially expressed genes (DEGs) in LD6A show a distant and diverse genetic nature due to their distant hybrid hexaploidy progeny. Further analysis revealed that most of the DEGs participated in the tricarboxylic acid (TCA) cycle, oxidative phosphorylation, histone acetyltransferase activity, sepal development, stigma development, cotyledon development and microsporogenesis. A highly differentially expressed toxic protein, Abrin, was identified in the CMS line LD6A, which can catalyze the inactivation of ribosomes and consequently lead to cell death through the mitochondrial pathway in human cells. Twelve DEGs were selected randomly to validate transcriptome data using quantitative reverse-transcribed PCR (qRT-PCR). This study will contribute to new ideas and foundations related to the molecular mechanism of CMS and the innovation of cotton germplasm resources.
Expression and Secretion of an Atrial Natriuretic Peptide in Beige-Like 3T3-L1 Adipocytes
Authors: Bae In-Seon , Kim Sang Hoon
Published: December 2019

The browning of white adipose tissue (beige adipocytes) stimulates energy expenditure. Omega-3 fatty acids have been shown to induce thermogenic action in adipocytes via G-protein coupled receptor 120 (GPR120). Atrial natriuretic peptide (ANP) is a peptide hormone that plays the role of maintaining normal blood pressure in kidneys to inhibit Na+ reuptake. Recently, ANP was found to induce adipocyte browning by binding to NPR1, an ANP receptor. However, the expression of ANP in adipocytes has not yet been studied. Therefore, in this study, we investigate the expression of ANP in beige-like adipocytes induced by docosahexaenoic acids (DHA), T3, or a PPAR agonist, rosiglitazone. First, we found that brown adipocyte-specific genes were upregulated in beige-like adipocytes. DHA promoted ANP expression in beige-like cells, whereas DHA-induced ANP expression was abolished by GPR120 knockout. ANP secretion of beige-like adipocytes was increased via PKC/ERK1/2 signaling in the GPR120 pathway. Furthermore, ANP secreted from beige-like adipocytes acted on HEK-293 cells, the recipient cells, leading to increased cGMP activity. After the NPR1 knockdown of HEK-293 cells, cGMP activity was not changed. Taken together, our findings indicate that beige-like adipocytes induce ANP secretion, which may contribute to improving obesity-associated metabolic disease.