bluuebunny/crossref_metadata_2025 huggingface dataset:
Capacity of Retinal Ganglion Cells Derived from Human Induced Pluripotent Stem Cells to Suppress T-Cells
Authors: Edo Ayaka , Sugita Sunao , Futatsugi Yoko , Sho Junki , Onishi Akishi , Kiuchi Yoshiaki , Takahashi Masayo
Published: October 2020

Retinal ganglion cells (RGCs) are impaired in patients such as those with glaucoma and optic neuritis, resulting in permanent vision loss. To restore visual function, development of RGC transplantation therapy is now underway. Induced pluripotent stem cells (iPSCs) are an important source of RGCs for human allogeneic transplantation. We therefore analyzed the immunological characteristics of iPSC-derived RGCs (iPSC-RGCs) to evaluate the possibility of rejection after RGC transplantation. We first assessed the expression of human leukocyte antigen (HLA) molecules on iPSC-RGCs using immunostaining, and then evaluated the effects of iPSC-RGCs to activate lymphocytes using the mixed lymphocyte reaction (MLR) and iPSC-RGC co-cultures. We observed low expression of HLA class I and no expression of HLA class II molecules on iPSC-RGCs. We also found that iPSC-RGCs strongly suppressed various inflammatory immune cells including activated T-cells in the MLR assay and that transforming growth factor-β2 produced by iPSC-RGCs played a critical role in suppression of inflammatory cells in vitro. Our data suggest that iPSC-RGCs have low immunogenicity, and immunosuppressive capacity on lymphocytes. Our study will contribute to predicting immune attacks after RGC transplantation.
Neonatal Seizures and Purinergic Signalling
Authors: Menéndez Méndez Aida , Smith Jonathon , Engel Tobias
Published: October 2020

Neonatal seizures are one of the most common comorbidities of neonatal encephalopathy, with seizures aggravating acute injury and clinical outcomes. Current treatment can control early life seizures; however, a high level of pharmacoresistance remains among infants, with increasing evidence suggesting current anti-seizure medication potentiating brain damage. This emphasises the need to develop safer therapeutic strategies with a different mechanism of action. The purinergic system, characterised by the use of adenosine triphosphate and its metabolites as signalling molecules, consists of the membrane-bound P1 and P2 purinoreceptors and proteins to modulate extracellular purine nucleotides and nucleoside levels. Targeting this system is proving successful at treating many disorders and diseases of the central nervous system, including epilepsy. Mounting evidence demonstrates that drugs targeting the purinergic system provide both convulsive and anticonvulsive effects. With components of the purinergic signalling system being widely expressed during brain development, emerging evidence suggests that purinergic signalling contributes to neonatal seizures. In this review, we first provide an overview on neonatal seizure pathology and purinergic signalling during brain development. We then describe in detail recent evidence demonstrating a role for purinergic signalling during neonatal seizures and discuss possible purine-based avenues for seizure suppression in neonates.
Structural Insights into the Interactions of Candidal Enolase with Human Vitronectin, Fibronectin and Plasminogen
Authors: Satala Dorota , Satala Grzegorz , Karkowska-Kuleta Justyna , Bukowski Michal , Kluza Anna , Rapala-Kozik Maria , Kozik Andrzej
Published: October 2020

Significant amounts of enolase—a cytosolic enzyme involved in the glycolysis pathway—are exposed on the cell surface of Candida yeast. It has been hypothesized that this exposed enolase form contributes to infection-related phenomena such as fungal adhesion to human tissues, and the activation of fibrinolysis and extracellular matrix degradation. The aim of the present study was to characterize, in structural terms, the protein-protein interactions underlying these moonlighting functions of enolase. The tight binding of human vitronectin, fibronectin and plasminogen by purified C. albicans and C. tropicalis enolases was quantitatively analyzed by surface plasmon resonance measurements, and the dissociation constants of the formed complexes were determined to be in the 10−7–10−8 M range. In contrast, the binding of human proteins by the S.cerevisiae enzyme was much weaker. The chemical cross-linking method was used to map the sites on enolase molecules that come into direct contact with human proteins. An internal motif 235DKAGYKGKVGIAMDVASSEFYKDGK259 in C. albicans enolase was suggested to contribute to the binding of all three human proteins tested. Models for these interactions were developed and revealed the sites on the enolase molecule that bind human proteins, extensively overlap for these ligands, and are well-separated from the catalytic activity center.
Early Diagnosis of Carotid Stenosis by Ultrasound Doppler Investigations: A Classification Method for the Hemodynamic Parameter
Authors: Xiao Huiyue , Zhang Yi , Yin Hao , Liu Paul , Liu Dong Chyuan
Published: October 2020

Pulsed Wave Doppler (PWD) is a traditional ultrasound technique used for the diagnosis of cardiovascular disease. The conventional diagnostic method is based on hemodynamic parameters obtained from the PW spectrum. However, it relies on the clinical experience of sonographers, and especially focusing on severe carotid stenosis. This paper proposes a classification method for the hemodynamic parameter using the RUSBoost algorithm. The proposed method improves the performance of RUSBoost by setting the empirical weight of each sample. The experimental results show that the proposed method reaches the accuracy of 90.1%, the sensitivity of 70%, and the specificity of 94%, which are 4%, 6%, and 2% higher than the original RUSBoost respectively. In addition, the proposed method is objective, since the empirical weights are computed based on Mahalanobis distance without any expert input. It can be used for the early detection of cardiovascular disease.
Development of the Physics–Based Morphology Model as the Platform for the Optimal Design of Beach Nourishment Project: A Numerical Study
Authors: Cho Yong Jun
Published: October 2020

In this study, a physics-based morphology model is developed and to test the feasibility of the morphology model proposed in this study as the platform for the optimal design of the beach nourishment project, the beach restoration process by the infra-gravity waves underlying the swells in a mild sea is numerically simulated. As a hydrodynamic module, the IHFOAM wave toolbox having its roots in the OpenFoam is used. Speaking of the morphology model, a transport equation for suspended load and the Exner type equation constitute the morphology model. In doing so, the probability theory first introduced by Einstein and the physical model test by Bagnold are used as the constituent sub-model of the morphology model. Numerical results show that among many flow features that are indispensable in forming sand bars over the flat bottom and swash zone, the partially skewed and asymmetric bottom shearing stresses, a shoreward Stokes drift near the free surface, boundary layer streaming near the seabed, and undertow toward the offshore were successfully simulated using the morphology model proposed in this study. It was also shown that plunging type breaker occurring at the final stage of the shoaling process, and its accompanying second breaker, sediment entrainment at the seabed, and the redistribution of suspended load by the down rush of preceding waves were successfully reproduced in the numerical simulation, and agreements with our experience in the field were very encouraging. In particular, the sand bar formation process over the flat bottom and backshore were successfully reproduced in the numerical simulation, which has been regarded as a challenging task.
Numerical Investigations on the Resistance and Longitudinal Motion Stability of a High-Speed Planing Trimaran
Authors: Sun Hanbing , Zou Jin , Sun Zhiyuan , Lu Shijie
Published: October 2020

The planing trimaran is a novel kind of aerodynamic alleviated marine vehicle and possesses a unique hybrid hydrodynamic and aerodynamic characteristics. In this paper, to investigate the hull behavior of a planing trimaran under the effect of tunnel forces, the tunnel and demihull are treated as appendages mounted on the slender main hull. Numerical simulations were carried out for planing trimaran and a monohull which was built according to the main hull configuration. Mesh convergence studies were implemented based on the public experimental data of a similar planing trimaran. Calculated results show that the presence of tunnel and demihull could decrease the resistance of the main hull and improve the longitudinal motion stability at high speeds. Flow evolutions of waves, velocity vector, wetted length and lift distribution were performed to explain the variation of the tunnel lift and its moment and their influence on hull behavior. Parameter studies on demihull length were carried out as well. It was found that, as the demihull length is increased, the tunnel trimming moment would decrease especially in the final stage of ventilation, making resistance reduced but the motion stability weakened.
Functional Defect of Neutrophils Causing Dermatophytosis: Case Report
Authors: Constantino-Silva Rosemeire N. , Perazzio Sandro F. , Weidebach Nicolas de Albuquerque , Grumach Anete S.
Published: October 2020

Background: NADPH-oxidase and myeloperoxidase (MPO) play an important role on defense against pathogenic microorganisms. Defects on these mechanisms have been described in association with recurrent infections due to such as Staphylococcus aureus and Candida albicans. We describe a patient with partial disturbance of intracellular microorganism destruction clinically manifested by recurrent fungal infection. Case report and results: A 58-year-old male rural farmer has suffered with superficial mycosis affecting hands, nails and right ankle persisting for 20 years. He was treated with several antifungal drugs with no improvement. Mycological scraping isolated Trichophyton rubrum. Immunological evaluation showed impaired T cell proliferation to Candidin and impaired neutrophil burst oxidative after specific stimulation with Candida albicans. The patient’s DNA was extracted from peripheral blood leukocytes for whole exome sequencing (WES) analysis. Two heterozygous variants of undetermined significance were screened accordingly: (1) MPO A332V (c.995G>A; rs28730837); and (2) NCF1 G83R (c.247G>A; rs139225348). Conclusions: Functional leukocyte evaluation with heterozygous variants in MPO and NCF1 suggest that these defects were associated with the susceptibility to dermatophytosis in our patient. We have developed a fast, effective and safe trial for screening individuals with yeast infections.
Production of Bio-Based Pigments from Food Processing Industry By-Products (Apple, Pomegranate, Black Carrot, Red Beet Pulps) Using Aspergillus carbonarius
Authors: Bezirhan Arikan Ezgi , Canli Oltan , Caro Yanis , Dufossé Laurent , Dizge Nadir
Published: October 2020

Food processing industry by-products (apple, pomegranate, black carrot, and red beet pulps) were evaluated as raw materials in pigment production by the filamentous fungi Aspergillus carbonarius. The effect of fermentation conditions (solid and submerged-state), incubation period (3, 6, 9, 12, and 15 d), initial substrate pH (4.5, 5.5, 6.5, 7.5, and 8.5), and pulp particle size (<1.4, 1.4–2.0, 2–4, and >4 mm) on fungal pigment production were tested to optimize the conditions. Pigment extraction analysis carried out under solid-state fermentation conditions showed that the maximum pigment production was determined as 9.21 ± 0.59 absorbance unit at the corresponding wavelength per gram (AU/g) dry fermented mass (dfm) for pomegranate pulp (PP) by A. carbonarius for 5 d. Moreover, the highest pigment production was obtained as 61.84 ± 2.16 AU/g dfm as yellowish brown at initial pH 6.5 with < 1.4 mm of substrate particle size for 15-d incubation period. GC×GC-TOFMS results indicate that melanin could be one of the main products as a pigment. SEM images showed that melanin could localize on the conidia of A. carbonarius.
Partnerships and Stakeholder Participation in the Management of National Parks: Experiences of the Gonarezhou National Park in Zimbabwe
Authors: Musakwa Walter , Gumbo Trynos , Paradza Gaynor , Mpofu Ephraim , Nyathi Nesisa Analisa , Selamolela Ntlakala B.
Published: October 2020

National parks play an important role in maintaining natural ecosystems which are important sources of income and livelihood sustenance. Most national parks in Southern Africa are managed by their states. Before 2007, Gonarezhou National Park was managed by the Zimbabwe Parks Management and Wildlife Authority, which faced challenges in maintaining its biodiversity, community relations and infrastructure. However, in 2017 the Frankfurt Zoological Society and the Zimbabwe Parks Management and Wildlife Authority formed an innovative partnership under the Gonarezhou Conservation Trust (GCT). This study examines the relationship between GCT management, Gonarezhou National Park stakeholders and communities as well as the impact of the relationship on biodiversity and ecosystems. The study also highlights challenges faced and lessons learned in managing Gonarezhou as a protected area. To obtain the information, key informant interviews, Landsat satellite imagery, secondary data from previous studies and government sources were utilized. The results indicate that the concerted efforts of the Gonarezhou Conservation Trust to manage the park are starting to bear fruit in improving biodiversity conservation, ecosystem management and engaging communities. However, challenges such as governance obstacles, problematic stakeholder management, maintaining trust in community relations, ensuring sustainability, managing the adverse impacts of climate change and human-wildlife conflicts must still be navigated to ensure the park’s sustainable management. Notwithstanding challenges, we argue that a partnership arrangement such as the Gonarezhou Conservation Trust is a desirable model that can be applied in national parks in Zimbabwe and Africa for better biodiversity management and tourism.
A New Shear Strength Criterion for Rock Masses with Non-Persistent Discontinuities Considering the Nonlinear Progressive Failure Process
Authors: Zheng Bowen , Qi Shengwen , Guo Songfeng , Huang Xiaolin , Liang Ning , Zou Yu , Luo Guangming
Published: October 2020

The shear strength characteristics of rock masses containing non-persistent discontinuities are strongly affected by discontinuities and rock bridges. The linear Jennings criterion cannot reflect the nonlinear mechanical behavior during progressive failure of rock masses with non-persistent discontinuities. In this study, a new nonlinear shear strength criterion was developed. First of all, a series of shear test data about artificial rock mass samples were collected on the basis of the published literatures, and five types of samples were differentiated according to the positions of discontinuities. After that, a new nonlinear shear strength criterion was proposed by introducing two correction coefficients A and B into the basic form of the Jennings criterion, which could correct the weight of the cohesion and the internal friction coefficient of rock bridges respectively. Then, the new criterion was determined by fitting the basic form of the Jennings criterion with the laboratory data. It was found that the parameters A and B had a nonlinear exponential and negative exponential relation with the connectivity rate respectively. It indicated that both the cohesion and the internal friction coefficient estimated by the new criterion were superior to those estimated by the Jennings criterion. Compared with the linear Jennings criterion, the new nonlinear shear strength criterion had a better applicability.
Collagen/PCL Nanofibers Electrospun in Green Solvent by DOE Assisted Process. An Insight into Collagen Contribution
Authors: Miele Dalila , Catenacci Laura , Rossi Silvia , Sandri Giuseppina , Sorrenti Milena , Terzi Alberta , Giannini Cinzia , Riva Federica , Ferrari Franca , Caramella Carla , Bonferoni Maria Cristina
Published: October 2020

Collagen, thanks to its biocompatibility, biodegradability and weak antigenicity, is widely used in dressings and scaffolds, also as electrospun fibers. Its mechanical stability can be improved by adding polycaprolactone (PCL), a synthetic and biodegradable aliphatic polyester. While previously collagen/PCL combinations were electrospun in solvents such as hexafluoroisopropanol (HFIP) or trifluoroethanol (TFE), more recently literature describes collagen/PCL nanofibers obtained in acidic aqueous solutions. A good morphology of the fibers represents in this case still a challenge, especially for high collagen/PCL ratios. In this work, thanks to preliminary rheological and physicochemical characterization of the solutions and to a Design of Experiments (DOE) approach on process parameters, regular and dimensionally uniform fibers were obtained with collagen/PCL ratios up to 1:2 and 1:1 w/w. Collagen ratio appeared relevant for mechanical strength of dry and hydrated fibers. WAXS and FTIR analysis showed that collagen denaturation is related both to the medium and to the electrospinning process. After one week in aqueous environment, collagen release was complete and a concentration dependent stimulatory effect on fibroblast growth was observed, suggesting the fiber suitability for wound healing. The positive effect of collagen on mechanical properties and on fibroblast biocompatibility was confirmed by a direct comparison of nanofiber performance after collagen substitution with gelatin.
Performance of Glass Wool Fibers in Asphalt Concrete Mixtures
Authors: Mrema Agathon Honest , Noh Si-Hyeon , Kwon Oh-Sun , Lee Jae-Jun
Published: October 2020

Nowadays, in order to improve asphalt pavement performance and durability and reduce environmental pollution caused by hydrocarbon materials, many researchers are studying different ways of modifying asphalt concrete (AC) and finding alternative paving materials to extend the service life of pavements. One of the successful materials used in the modification of AC is fibers. Different types of fibers have been reinforced in AC mixtures and improvements have been observed. This research studies the performance of glass wool fibers reinforced in a dense-graded asphalt mixture. Generally, glass fibers are known to have excellent mechanical properties such as high tensile modulus, 100% elastic recovery and a very high tolerance to heat. Glass wool fibers are commonly used as a thermal insulation material. In this research, to evaluate the performance of glass wool fibers in AC, laboratory tests, the Marshall mix design test, indirect tensile strength (IDT), tensile strength ratio (TSR) and the Kim test were conducted to determine a proper mix design, tensile properties, moisture susceptibility, rutting and fatigue behaviors. Results show that the addition of glass wool fibers does affect the properties of AC mixtures. The use of glass wool fibers shows a positive consistence result, in which it improved the moisture susceptibility and rutting resistance of the AC. Additionally, results show that the addition of fiber increased tensile strength and toughness which indicates that fibers have a potential to resist distresses that occur on a surface of the road as a result of heavy traffic loading. The overall results show that the addition of glass wool fibers in AC mixtures is beneficial in improving properties of AC pavements.
Diatomite-Metal-Organic Framework Composite with Hierarchical Pore Structures for Adsorption/Desorption of Hydrogen, Carbon Dioxide and Water Vapor
Authors: Wang Gaofeng , Graham Elizabeth , Zheng Shuilin , Zhu Jianxi , Zhu Runliang , He Hongping , Sun Zhiming , Mackinnon Ian D. R. , Xi Yunfei
Published: October 2020

Distinctive Cr-MOF@Da composites have been constructed using chromium-based metal-organic frameworks (MOFs) and diatomite (Da). The new materials have hierarchical pore structures containing micropores, mesopores and macropores. We have synthesized various morphologies of the MOF compound Cr-MIL-101 to combine with Da in a one-pot reaction step. These distinctive hierarchical pore networks within Cr-MIL-101@Da enable exceptional adsorptive performance for a range of molecules, including hydrogen (H2), carbon dioxide (CO2) and water (H2O) vapor. Selectivity for H2 or CO2 can be moderated by the morphology and composition of the Cr-MIL-101 included in the Cr-MOF@Da composite. The encapsulation and growth of Cr-MIL-101 within and on Da have resulted in excellent water retention as well as high thermal and hydrolytic stability. In some cases, Cr-MIL-101@Da composite materials have demonstrated increased thermal stability compared with that of Cr-MIL-101; for example, decomposition temperatures >340 ℃ can be achieved. Furthermore, these Cr-MIL-101@Da composites retain structural and morphological integrity after 60 cycles of repeated hydration/dehydration, and after storage for more than one year. These characteristics are difficult to achieve with many MOF materials, and thus suggest that MOF–mineral composites show high potential for practical gas storage and water vapor capture.
Micro Plastic Deformation Behavior of Carbide-Free Bainitic Steel during Tensile Deformation Process
Authors: Zhang Ming , Yang Xiaowu , Kang Jie , Zhang Fucheng , Yang Zhinan
Published: October 2020

The microstructure of carbide-free bainitic steel has been widely reported because of its excellent mechanical properties. However, no work has explored the deformation behavior of each phase in the microstructure, namely, retained austenite (RA) and bainitic ferrite (BF). In this paper, the deformation behavior of RA and BF during tension was investigated through the in situ electron backscatter diffusion method. The results showed that the RA surrounded by BF with a high Schmid factor (SF) was accelerated towards being transformed into martensite, but the surrounding BF with low SF value retarded the transformation. The kernel average misorientation (KAM) findings revealed that the strain of the RA and BF rapidly increased initially, slowed down after the critical points of 0.25 and 0.54, respectively, and then increased again. The calculated result for the KAM fraction indicated that the contribution of RA to the total plastic deformation gradually decreased throughout the total tensile process, whereas that of BF increased in the presence of RA.
Prediction of Fusion Hole Perforation Based on Arc Characteristics of Front Image in Backing Welding
Authors: Cao Yu , Wang Xiaofei , Yan Xu , Jia Chuanbao , Gao Jinqiang
Published: October 2020

In one-side welding with back-formation, the weld is penetrated after the fusion hole is perforated. Therefore, judging whether the fusion hole is perforated is very important to realize autocontrol of penetration in one-side welding with back-formation process. Previous researches mainly focused on the morphological characteristics of the molten pool and fusion hole to judge the weld penetration state. Sometimes it is difficult to obtain the morphological characteristics of the molten pool, keyhole and fusion hole and image processing is complex. In this paper, a visual detection system of fusion holes based on visual sensing is constructed to obtain the real-time fusion hole images in backing welding. It is found that the arc characteristics in the front images contain abundant information about the perforation of fusion hole. An image processing program is developed based on MATLAB software, and the arc characteristic parameters in front images are obtained. Taking the arc characteristic parameters as the input, obtaining the penalty function and the kernel function parameters through the cross validation and grid search method, a prediction model of fusion hole perforation based on the support vector machine is put forward. The accuracy for prediction samples is 88%. By analyzing the misidentified samples, it is found that some of the newly perforated images are predicted as nonperforated ones, which has little influence on the penetration control of the weld.
The Impact of Hemp Shives Impregnated with Selected Plant Oils on Mechanical, Thermal, and Insulating Properties of Polyurethane Composite Foams
Authors: Członka Sylwia , Strąkowska Anna , Kairytė Agnė
Published: October 2020

Polyurethane (PUR) foams reinforced with 2 wt.% hemp shives (HS) fillers were successfully synthesized. Three different types of HS fillers were evaluated—non-treated HS, HS impregnated with sunflower oil (SO) and HS impregnated with tung oil (TO). The impact of each type of HS fillers on cellular morphology, mechanical performances, thermal stability, and flame retardancy was evaluated. It has been shown that the addition of HS fillers improved the mechanical characteristics of PUR foams. Among all modified series, the greatest improvement was observed after the incorporation of non-treated HS filler—when compared with neat foams, the value of compressive strength increased by ~13%. Moreover, the incorporation of impregnated HS fillers resulted in the improvement of thermal stability and flame retardancy of PUR foams. For example, the addition of both types of impregnated HS fillers significantly decreased the value of heat peak release (pHRR), total smoke release (TSR), and limiting oxygen index (LOI). Moreover, the PUR foams containing impregnated fillers were characterized by improved hydrophobicity and limited water uptake. The obtained results confirmed that the modification of PUR foams with non-treated and impregnated HS fillers may be a successful approach in producing polymeric composites with improved properties.
Effect of Laser Shock Peening on Fretting Fatigue Life of TC11 Titanium Alloy
Authors: Yang Xufeng , Zhang Hongjian , Cui Haitao , Wen Changlong
Published: October 2020

The purpose of this paper is to investigate the performance of laser shock peening (LSP) subjected to fretting fatigue with TC11 titanium alloy specimens and pads. Three laser power densities (3.2 GW/cm2, 4.8 GW/cm2 and 6.4 GW/cm2) of LSP were chosen and tested using manufactured fretting fatigue apparatus. The experimental results show that the LSP surface treatment significantly improves the fretting fatigue lives of the fretting specimens, and the fretting fatigue life increases most when the laser power density is 4.8 GW/cm2. It is also found that with the increase of the laser power density, the fatigue crack initiation location tends to move from the surface to the interior of the specimen.
Hydrothermally Assisted Fabrication of TiO2-Fe3O4 Composite Materials and Their Antibacterial Activity
Authors: Kubiak Adam , Kubacka Marta , Gabała Elżbieta , Dobrowolska Anna , Synoradzki Karol , Siwińska-Ciesielczyk Katarzyna , Czaczyk Katarzyna , Jesionowski Teofil
Published: October 2020

The TiO2-Fe3O4 composite materials were fabricated via the hydrothermal-assisted technique. It was determined how the molar ratio of TiO2 to Fe3O4 influences the crystalline structure and morphology of the synthesized composite materials. The effect of the molar ratio of components on the antibacterial activity was also analyzed. On the basis of XRD patterns for the obtained titanium(IV) oxide-iron(II, III) oxide composites, the two separate crystalline forms—anatase and magnetite —were observed. Transmission electron microscopy revealed particles of cubic and tetragonal shape for TiO2 and spherical for Fe3O4. The results of low-temperature nitrogen sorption analysis indicated that an increase in the iron(II, III) oxide content leads to a decrease in the BET surface area. Moreover, the superparamagnetic properties of titanium(IV) oxide-iron(II, III) oxide composites should be noted. An important aim of the work was to determine the antibacterial activity of selected TiO2-Fe3O4 materials. For this purpose, two representative strains of bacteria, the Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, were used. The titanium(IV) oxide-iron(II, III) oxide composites demonstrated a large zone of growth inhibition for both Gram-positive and Gram-negative bacteria. Moreover, it was found that the analyzed materials can be reused as antibacterial agents in three consecutive cycles with good results.
Analysis of Notch Effect in 3D-Printed ABS Fracture Specimens Containing U-Notches
Authors: Cicero Sergio , Martínez-Mata Victor , Alonso-Estebanez Alejandro , Castanon-Jano Laura , Arroyo Borja
Published: October 2020

In this paper a fracture assessment in additive manufactured acrylonitrile butadiene styrene (ABS) fracture specimens containing U-notches is performed. We performed 33 fracture tests and 9 tensile tests, combining five different notch radii (0 mm, 0.25 mm, 0.50 mm, 1 mm and 2 mm) and three different raster orientations: 0/90, 30/−60 and 45/−45. The theory of critical distances (TCD) was then used in the analysis of fracture test results, obtaining additional validation of this theoretical framework. Different versions of TCD provided suitable results contrasting with the experimental tests performed. Moreover, the fracture mechanisms were evaluated using scanning electron microscopy in order to establish relationships with the behaviour observed. It was demonstrated that 3D-printed ABS material presents a clear notch effect, and also that the TCD, through both the point method and the line method, captured the physics of the notch effect in 3D-printed ABS. Finally, it was observed that the change in the fracture mechanisms when introducing a finite notch radius was limited to a narrow band behind the original defect, which appeared in cracked specimens but not in notched specimens.
Separation of Fructose and Glucose via Nanofiltration in Presence of Fructooligosaccharides
Authors: Rizki Zulhaj , Janssen Anja , van der Padt Albert , Boom Remko
Published: October 2020

Fructose and glucose are commonly present together in mixtures and may need to be separated. Current separation methods for these isomers are complex and costly. Nanofiltration is a cost-effective method that has been widely used for separating carbohydrates of different sizes; however, it is not commonly used for such similar molecules. Here, we report the separation of fructose and glucose in a nanofiltration system in the presence of fructooligosaccharides (FOS). Experiments were performed using a pilot-scale filtration setup using a spiral wound nanofiltration membrane with molecular weight cutoff of 1 kDa. We observed three important factors that affected the separation: (1) separation of monosaccharides only occurred in the presence of FOS and became more effective when FOS dominated the solution; (2) better separation was achieved when the monosaccharides were mainly fructose; and (3) the presence of salt improved the separation only moderately. The rejection ratio (Rf/Rg) in a fructose/glucose mixture is 0.92. We reported a rejection ratio of 0.69, which was observed in a mixture of 50 g/L FOS with a fructose to glucose ratio of 4.43. The separation is hypothesized to occur due to selective transport in the FOS layer, resulting in a preferential binding towards fructose.
Thermomechanical Processing of Cost-Affordable Powder Metallurgy Ti-5Fe Alloys from the Blended Elemental Approach: Microstructure, Tensile Deformation Behavior, and Failure
Authors: Romero Carlos , Yang Fei , Wei Shanghai , Bolzoni Leandro
Published: October 2020

The development of cost-affordable Ti alloys is key for the application of Ti in other industries like the automobile sector. Therefore, a combination of powder metallurgy (PM) and low-cost compositions is an interesting approach. In this article, a cost-affordable PM Ti-5Fe alloy is processed following the blended elemental route and extruded at high temperature to remove porosity. Different extrusion temperatures and heat treatments (i.e., solution treatment and aging, STA) are performed to obtain ultrafine microstructures, and their effect on the mechanical behavior is studied. For extrusions in the β phase, microstructures consist of coarse lamellar colonies, resulting in alloys with improved properties compared to the as-sintered alloy but still lacking toughness due to the failure happening just after necking onset. Extruding in the α + β phase results in a bimodal microstructure of fine elongated primary α and coarse lamellar colonies, and the alloy becomes tougher. STA with aging below the eutectoid temperature of 590 °C leads to a hard but brittle alloy, whereas STA with aging above it results in alloys with strength comparable to the as-extruded conditions and enhanced ductility.
Xenon Flash Lamp Lift-Off Technology without Laser for Flexible Electronics
Authors: Lee Sang Il , Jang Seong Hyun , Han Young Joon , Lee Jun yeub , Choi Jun , Cho Kwan Hyun
Published: October 2020

This study experimentally investigated process mechanisms and characteristics of newly developed xenon flash lamp lift-off (XF-LO) technology, a novel thin film lift-off method using a light to heat conversion layer (LTHC) and a xenon flash lamp (XFL). XF-LO technology was used to lift-off polyimide (PI) films of 8.68–19.6 μm thickness. When XFL energy irradiated to the LTHC was 2.61 J/cm2, the PI film was completely released from the carrier substrate. However, as the energy intensity of the XFL increased, it became increasingly difficult to completely release the PI film from the carrier substrate. Using thermal gravimetric analysis (TGA), Fourier-transform infrared spectroscopy (FTIR) and transmittance analysis, the process mechanism of XF-LO technology was investigated. Thermal durability of the PI film was found to deteriorate with increasing XFL energy intensity, resulting in structural deformation and increased roughness of the PI film surface. The optimum energy intensity of 2.61 J/cm2 or less was found to be effective for performing XF-LO technology. This study provides an attractive method for manufacturing flexible electronic boards outside the framework of existing laser lift-off (LLO) technology.
A Multi-Omic Analysis for Low Bone Mineral Density in Postmenopausal Women Suggests a Relationship between Diet, Metabolites, and Microbiota
Authors: Palacios-González Berenice , Ramírez-Salazar Eric G. , Rivera-Paredez Berenice , Quiterio Manuel , Flores Yvonne N. , Macias-Kauffer Luis , Moran-Ramos Sofía , Denova-Gutiérrez Edgar , Ibarra-González Isabel , Vela-Amieva Marcela , Canizales-Quinteros Samuel , Salmerón Jorge , Velázquez-Cruz Rafael
Published: October 2020

The effect of microbiota composition and its health on bone tissue is a novel field for research. However, their associations with bone mineral density (BMD) have not been established in postmenopausal women. The present study investigates the relation of diet, the microbiota composition, and the serum metabolic profile in postmenopausal women with normal-BMD or with low-BMD. Ninety-two Mexican postmenopausal women were classified into normal-BMD (n = 34) and low-BMD (n = 58). The V4 hypervariable region was sequenced using the Miseq platform. Serum vitamin D was determined by chemiluminescence immunoassay. Serum concentrations of acyl-carnitines and amino acids were determined by electrospray tandem mass spectrometry. Diet was assessed by a food frequency questionnaire. The low-BMD group had fewer observed species, higher abundance of γ-Proteobacteria, lower consumption of lycopene, and lower concentrations of leucine, valine, and tyrosine compared with the normal-BMD group. These amino acids correlated positively with the abundance of Bacteroides. Lycopene consumption positively correlated with Oscillospira and negatively correlated with Pantoea genus abundance. Finally, the intestinal microbiota of women with vitamin D deficiency was related to Erysipelotrichaceae and Veillonellaceae abundance compared to the vitamin D non-deficient group. Associations mediated by the gut microbiota between diet and circulating metabolites with low-BMD were identified.
Rapid Changes in Microbial Community Structures along a Meandering River
Authors: Cruaud Perrine , Vigneron Adrien , Dorea Caetano C. , Rodriguez Manuel J. , Charette Steve J.
Published: October 2020

Streams and rivers convey freshwater from lands to the oceans, transporting various organic particles, minerals, and living organisms. Microbial communities are key components of freshwater food webs and take up, utilize, and transform this material. However, there are still important gaps in our understanding of the dynamic of these organisms along the river channels. Using high-throughput 16S and 18S rRNA gene sequencing and quantitative PCR on a 11-km long transect of the Saint-Charles River (Quebec, CA), starting from its main source, the Saint-Charles Lake, we show that bacterial and protist community structures in the river drifted quickly but progressively downstream of its source. The dominant Operational Taxonomic Units (OTUs) of the lake, notably related to Cyanobacteria, decreased in proportions, whereas relative proportions of other OTUs, such as a Pseudarcicella OTU, increased along the river course, becoming quickly predominant in the river system. Both prokaryotic and protist communities changed along the river transect, suggesting a strong impact of the shift from a stratified lake ecosystem to a continuously mixed river environment. This might reflect the cumulative effects of the increasing water turbulence, fluctuations of physicochemical conditions, differential predation pressure in the river, especially in the lake outlet by benthic filter feeders, or the relocation of microorganisms, through flocculation, sedimentation, resuspension, or inoculation from the watershed. Our study reveals that the transit of water in a river system can greatly impact both bacterial and micro-eukaryotic community composition, even over a short distance, and, potentially, the transformation of materials in the water column.
Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future Methods
Authors: Reimão Juliana Quero , Coser Elizabeth Magiolo , Lee Monica Ran , Coelho Adriano Cappellazzo
Published: October 2020

Leishmaniasis is a neglected tropical disease with two main clinical forms: cutaneous and visceral leishmaniasis. Diagnosis of leishmaniasis is still a challenge, concerning the detection and correct identification of the species of the parasite, mainly in endemic areas where the absence of appropriate resources is still a problem. Most accessible methods for diagnosis, particularly in these areas, do not include the identification of each one of more than 20 species responsible for the disease. Here, we summarize the main methods used for the detection and identification of leishmaniasis that can be performed by demonstration of the parasite in biological samples from the patient through microscopic examination, by in vitro culture or animal inoculation; by molecular methods through the detection of parasite DNA; or by immunological methods through the detection of parasite antigens that may be present in urine or through the detection of specific antibodies against the parasite. Potential new methods that can be applied for laboratory diagnosis of leishmaniasis are also discussed.
Revisiting an Aspergillus flavus Strain Isolated from an Egyptian Sugarcane Field in 1930
Authors: Abdallah Mohamed F. , Audenaert Kris , Saeger Sarah De , Houbraken Jos
Published: October 2020

The aflatoxin type B and G producer Aspergillus novoparasiticus was described in 2012 and was firstly reported from sputum, hospital air (Brazil), and soil (Colombia). Later, several survey studies reported the occurrence of this species in different foods and other agricultural commodities from several countries worldwide. This short communication reports on an old fungal strain (CBS 108.30), isolated from Pseudococcus sacchari (grey sugarcane mealybug) from an Egyptian sugarcane field in (or before) 1930. This strain was initially identified as Aspergillus flavus; however, using the latest taxonomy schemes, the strain is, in fact, A. novoparasiticus. These data and previous reports indicate that A. novoparasiticus is strongly associated with sugarcane, and pre-harvest biocontrol approaches with non-toxigenic A. novoparasiticus strains are likely to be more successful than those using non-toxigenic A. flavus strains. Further studies on the association between A. novoparasiticus and Pseudococcus sacchari might shed light on the distribution (and aflatoxin contamination) of this species in sugarcane. Additionally, the interaction between A. novoparasiticus, Pseudococcus sacchari, and sugarcane crop under different scenarios of climate change will be critical in order to get more insight into the host–pathogen interaction and host resistance and propose appropriate prevention strategies to decrease mycotoxin contamination and crop loss due to A. novoparasiticus attack.
Innovative and Assistive eHealth Technologies for Smart Therapeutic and Rehabilitation Outdoor Spaces for the Elderly Demographic
Authors: Marques Bruno , McIntosh Jacqueline , Valera Alvin , Gaddam Anuroop
Published: October 2020

The use of technology for social connectivity and achieving engagement goals is increasingly essential to the overall well-being of our rapidly ageing population. While much of the extant literature has focused on home automation and indoor remote health monitoring; there is a growing literature that finds personal health and overall well-being improves when physical activities are conducted outdoors. This study presents a review of possible innovative and assistive eHealth technologies suitable for smart therapeutic and rehabilitation outdoor spaces for older persons. The article also presents key performance metrics required of eHealth technologies to ensure robust, timely and reliable biometric data transfer between patients in a therapeutic landscape environment and respective medical centres. A literature review of relevant publications with a primary focus of integrating sensors and eHealth technologies in outdoor spaces to collect and transfer data from the elderly demographic who engage such built landscapes to appropriate stakeholders was conducted. A content analysis was carried out to synthesize outcomes of the literature review. The study finds that research in assistive eHealth technologies and interfaces for outdoor therapeutic spaces is in its nascent stages and has limited generalisability. The level of technology uptake and readiness for smart outdoor spaces is still developing and is currently being outpaced by the growth of elderly fitness zones in public spaces. Further research is needed to explore those eHealth technologies with interactive feedback mechanisms that are suitable for outdoor therapeutic environments.
How Thermal Aging Affects Ignition and Combustion Properties of Reactive Al/CuO Nanolaminates: A Joint Theoretical/Experimental Study
Authors: Estève A. , Lahiner G. , Julien B. , Vivies S. , Richard N. , Rossi C.
Published: October 2020

The paper reports a joint experimental/theoretical study on the aging of reactive Al/CuO nanolaminates, investigating both structural modifications and combustion properties of aged systems. We first show theoretically that the long-term storage (over several decades) in ambient temperature marginally affects nanolaminates structural properties with an increase in an interfacial layer of only 0.3 nm after 30 years. Then, we observe that the first thermal aging step occurs after 14 days at 200 °C, which corresponds to the replacement of the natural Al/CuO interfaces by a proper ~11 nm thick amorphous alumina. We show that this aging step does impact the nanolaminates structure, leading, for thin bilayer thicknesses, to a substantial loss of the energetic reservoir: considering a stoichiometric Al/CuO stack, the heat of reaction can be reduced by 6–40% depending on the bilayer thickness ranging from 150 nm (40%) to 1 µm (6%). The impact of such thermal aging (14 days at 200 °C) and interfacial modification on the initiation and combustion properties have been evaluated experimentally and theoretically. Varying Al to CuO ratio of nanolaminates from 1 to 3, we show that ignition time of aged systems does not increase over 10% at initiation power densities superior to 15 W·mm−2. In contrast, burn rate can be greatly impacted depending on the bilayer thickness: annealing a stoichiometric nanolaminates with a bilayer thickness of 300 nm at 200 °C for 14 days lowers its burn rate by ~25%, whereas annealing a fuel rich nanolaminates with the same bilayer thickness under the same thermal conditions leads to a burn rate decrease of 20%. When bilayer thickness is greater than 500 nm, the burn rate is not really affected by the thermal aging. Finally, this paper also proposes a time–temperature diagram to perform accelerated thermal aging.
Spin Caloritronics in 3D Interconnected Nanowire Networks
Authors: da Câmara Santa Clara Gomes Tristan , Marchal Nicolas , Abreu Araujo Flavio , Piraux Luc
Published: October 2020

Recently, interconnected nanowire networks have been found suitable as flexible macroscopic spin caloritronic devices. The 3D nanowire networks are fabricated by direct electrodeposition in track-etched polymer templates with crossed nano-channels. This technique allows the fabrication of crossed nanowires consisting of both homogeneous ferromagnetic metals and multilayer stack with successive layers of ferromagnetic and non-magnetic metals, with controlled morphology and material composition. The networks exhibit extremely high, magnetically modulated thermoelectric power factors. Moreover, large spin-dependent Seebeck coefficients were directly extracted from experimental measurements on multilayer nanowire networks. This work provides a simple and cost-effective way to fabricate large-scale flexible and shapeable thermoelectric devices exploiting the spin degree of freedom.
Analog and Digital Bipolar Resistive Switching in Co–Al-Layered Double Hydroxide Memristor
Authors: Sun Yanmei , Li Li , Shi Keying
Published: October 2020

We demonstrate a nonvolatile memristor based on Co–Al-layered double hydroxide (Co–Al LDH). We also introduce a memristor that has a hexazinone-adsorbing Co–Al LDH composite active layer. Memristor characteristics could be modulated by adsorbing hexazinone with Co–Al LDHs in the active layer. While different, Co–Al LDH-based memory devices show gradual current changes, and the memory device with small molecules of adsorbed hexazinone undergo abrupt changes. Both devices demonstrate programmable memory peculiarities. In particular, both memristors show rewritable resistive switching with electrical bistability (>105 s). This research manifests the promising potential of 2D nanocomposite materials for adsorbing electroactive small molecules and rectifying resistive switching properties for memristors, paving a way for design of promising 2D nanocomposite memristors for advanced device applications.
Morphology and Mechanical Properties of 3Y-TZP Nanofiber Mats
Authors: Tyurin Alexander I. , Rodaev Vyacheslav V. , Razlivalova Svetlana S. , Korenkov Viktor V. , Zhigachev Andrey O. , Vasyukov Vladimir M. , Golovin Yuri I.
Published: October 2020

The mats of yttria-stabilized tetragonal zirconia nanofibers were prepared using electrospinning. The effect of calcination temperature in the range of 600–1200 °C on their microstructure, phase composition and mechanical properties was investigated. Phase composition of the nanofibers did not change in all ranges of the calcination temperatures, while the average grain size increased from 8 to 39 nm. Nanoindentation testing of the mats showed a decrease in the hysteresis loop energy in samples with higher calcination temperature. Hardness and the elastic modulus measured with the indentation technique were the highest for the mats calcined at 900 °C.