bluuebunny/crossref_metadata_2025 huggingface dataset:
Study on the Curing and Foaming of Surfactant-Modified Geopolymer Gels Based on Ash and Slag Waste from Coal Combustion
Authors: Yatsenko Elena A. , Trofimov Sergei V. , Goltsman Boris M. , Li Wensheng , Smoliy Victoria A. , Ryabova Anna V. , Klimova Lyudmila V. , Izvarin Andrey I.
Published: December 2023

This study explores the influence of temperature–time conditions, surfactants, and varied waste compositions on the curing of geopolymer gels, a foam formation with the properties of porous geopolymers. Findings reveal that a 6 h curing period leads to a density of 435 kg/m3 and strength of 0.66 MPa, with notable improvements at 12 h. Comparing 12 to 24 h curing, differences in characteristics remain within 5%, highlighting the 12 h period as more energy-efficient. Sodium stearate-based samples exhibit excellent properties, significantly boosting strength while maintaining overall properties. Microwave curing achieves the lowest density (291 kg/m3) and closely parallels properties of samples cured conventionally for 12 h. However, it leads to complete destruction in sodium stearate-modified gels due to the Dumas reaction, making it unsuitable above 200 °C. Optimal properties emerge from compositions using sodium stearate and oven curing, achieving densities of 334 kg/m3 and strengths of 1.08 MPa (Severodvinsk CHPP-1) and 373 kg/m3 and 1.17 MPa (Novocherkassk SDPP). Although microwave curing allows for high energy efficiency, its high temperature demands necessitate careful material selection. This study offers insight into enhancing geopolymer properties while emphasizing the importance of tailored curing methods for sustainable material development.
Rare Deletions or Large Duplications Contribute to Genetic Variation in Patients with Severe Tinnitus and Meniere Disease
Authors: Escalera-Balsera Alba , Parra-Perez Alberto M. , Gallego-Martinez Alvaro , Frejo Lidia , Martin-Lagos Juan , Rivero de Jesus Victoria , Pérez-Vázquez Paz , Perez-Carpena Patricia , Lopez-Escamez Jose A.
Published: December 2023

Meniere disease (MD) is a debilitating disorder of the inner ear defined by sensorineural hearing loss (SNHL) associated with episodes of vertigo and tinnitus. Severe tinnitus, which occurs in around 1% of patients, is a multiallelic disorder associated with a burden of rare missense single nucleotide variants in synaptic genes. Rare structural variants (SVs) may also contribute to MD and severe tinnitus. In this study, we analyzed exome sequencing data from 310 MD Spanish patients and selected 75 patients with severe tinnitus based on a Tinnitus Handicap Inventory (THI) score > 68. Three rare deletions were identified in two unrelated individuals overlapping the ERBB3 gene in the positions: NC_000012.12:g.56100028_56100172del, NC_000012.12:g.56100243_56101058del, and NC_000012.12:g.56101359_56101526del. Moreover, an ultra-rare large duplication was found covering the AP4M1, COPS6, MCM7, TAF6, MIR106B, MIR25, and MIR93 genes in another two patients in the NC_000007.14:g.100089053_100112257dup region. All the coding genes exhibited expression in brain and inner ear tissues. These results confirm the contribution of large SVs to severe tinnitus in MD and pinpoint new candidate genes to get a better molecular understanding of the disease.
Genome-Wide Identification and Expression Analysis of the SBP-Box Gene Family in Loquat Fruit Development
Authors: Song Haiyan , Zhao Ke , Jiang Guoliang , Sun Shuxia , Li Jing , Tu Meiyan , Wang Lingli , Xie Hongjiang , Chen Dong
Published: December 2023

The loquat (Eriobotrya japonica L.) is a special evergreen tree, and its fruit is of high medical and health value as well as having stable market demand around the world. In recent years, research on the accumulation of nutrients in loquat fruit, such as carotenoids, flavonoids, and terpenoids, has become a hotspot. The SBP-box gene family encodes transcription factors involved in plant growth and development. However, there has been no report on the SBP-box gene family in the loquat genome and their functions in carotenoid biosynthesis and fruit ripening. In this study, we identified 28 EjSBP genes in the loquat genome, which were unevenly distributed on 12 chromosomes. We also systematically investigated the phylogenetic relationship, collinearity, gene structure, conserved motifs, and cis-elements of EjSBP proteins. Most EjSBP genes showed high expression in the root, stem, leaf, and inflorescence, while only five EjSBP genes were highly expressed in the fruit. Gene expression analysis revealed eight differentially expressed EjSBP genes between yellow- and white-fleshed fruits, suggesting that the EjSBP genes play important roles in loquat fruit development at the breaker stage. Notably, EjSBP01 and EjSBP19 exhibited completely opposite expression patterns between white- and yellow-fleshed fruits during fruit development, and showed a close relationship with SlCnr involved in carotenoid biosynthesis and fruit ripening, indicating that these two genes may participate in the synthesis and accumulation of carotenoids in loquat fruit. In summary, this study provides comprehensive information about the SBP-box gene family in the loquat, and identified two EjSBP genes as candidates involved in carotenoid synthesis and accumulation during loquat fruit development.
Association of Variants in Innate Immune Genes TLR4 and TLR5 with Reproductive and Milk Production Traits in Czech Simmental Cattle
Authors: Novák Karel , Valčíková Terezie , Samaké Kalifa , Bjelka Marek
Published: December 2023

Bovine genes TLR4 and TLR5, which encode antibacterial toll-like receptors, were screened for polymorphisms in Czech Red Pied (Czech Simmental) cattle to identify variants associated with reproduction, udder health, and milk production traits. Variants were discovered by hybrid resequencing of 164 bulls using HiSeq X-Ten and PacBio technologies and then individually genotyped. Nominal p-values < 0.05 for associations were detected in 18 combinations between 14 polymorphisms and 15 traits using one-way analysis of variance (ANOVA). The TLR4 variants g.610C>T (rs43578094) and g.10310T>G (rs8193072) in reference AC000135.1 were strictly associated with the index of early reproductive disorders and maternal calving ease, respectively, at false discovery rate (FDR) < 0.05. A highly permissive false discovery rate cutoff of 0.6 separated seventeen combinations in both genes comprising eight positives. In the case of the TLR4 variant g.9422T>C (rs8193060), indications were obtained for the association with as many as four reproductive traits: incidence of cystic ovaries, early reproductive disorders, calving ease, and production longevity. The permissive FDR interpretation for the TLR5 data indicated associations with cyst incidence and early reproduction disorders with maternal calving ease. Moreover, three TLR5 polymorphisms correlated with milk production traits. The discrepancy of the observed associations with the predicted impacts of the SNPs on protein function points to the role of haplotypes. Nevertheless, this question should be resolved on a larger scale. The observed associations are endorsed by independent evidence from the published functional roles in other species and by the published QTL mapping data.
When Protein Structure Embedding Meets Large Language Models
Authors: Ali Sarwan , Chourasia Prakash , Patterson Murray
Published: December 2023

Protein structure analysis is essential in various bioinformatics domains such as drug discovery, disease diagnosis, and evolutionary studies. Within structural biology, the classification of protein structures is pivotal, employing machine learning algorithms to categorize structures based on data from databases like the Protein Data Bank (PDB). To predict protein functions, embeddings based on protein sequences have been employed. Creating numerical embeddings that preserve vital information while considering protein structure and sequence presents several challenges. The existing literature lacks a comprehensive and effective approach that combines structural and sequence-based features to achieve efficient protein classification. While large language models (LLMs) have exhibited promising outcomes for protein function prediction, their focus primarily lies on protein sequences, disregarding the 3D structures of proteins. The quality of embeddings heavily relies on how well the geometry of the embedding space aligns with the underlying data structure, posing a critical research question. Traditionally, Euclidean space has served as a widely utilized framework for embeddings. In this study, we propose a novel method for designing numerical embeddings in Euclidean space for proteins by leveraging 3D structure information, specifically employing the concept of contact maps. These embeddings are synergistically combined with features extracted from LLMs and traditional feature engineering techniques to enhance the performance of embeddings in supervised protein analysis. Experimental results on benchmark datasets, including PDB Bind and STCRDAB, demonstrate the superior performance of the proposed method for protein function prediction.
The Mutational Spectrum of Pre- and Post-Neoadjuvant Chemotherapy Triple-Negative Breast Cancers
Authors: Aguilar-Mahecha Adriana , Alirezaie Najmeh , Lafleur Josiane , Bareke Eric , Przybytkowski Ewa , Lan Cathy , Cavallone Luca , Salem Myriam , Pelmus Manuela , Aleynikova Olga , Greenwood Celia , Lovato Amanda , Ferrario Cristiano , Boileau Jean-François , Mihalcioiu Catalin , Roy Josée-Anne , Marcus Elizabeth , Discepola Federico , Majewski Jacek , Basik Mark
Published: December 2023

The response of triple-negative breast cancer (TNBC) patients to pre-operative (neoadjuvant chemotherapy) is a critical factor of their outcome. To determine the effects of chemotherapy on the tumor genome and to identify mutations associated with chemoresistance and sensitivity, we performed whole exome sequencing on pre/post-chemotherapy tumors and matched lymphocytes from 26 patients. We observed great inter-tumoral heterogeneity with no gene mutated recurrently in more than four tumors besides TP53. Although the degree of response to chemotherapy in residual tumors was associated with more subclonal changes during chemotherapy, there was minimal evolution between pre/post-tumors. Indeed, gene sets enriched for mutations in pre- and post-chemotherapy tumors were very similar and reflected genes involved in the biological process of neurogenesis. Somatically mutated genes present in chemosensitive tumors included COL1A2, PRMD15, APOBEC3B, PALB2 and histone protein encoding genes, while BRCA1, ATR, ARID1A, XRCC3 and genes encoding for tubulin-associated proteins were present in the chemoresistant tumors. We also found that the mutational spectrum of post-chemotherapy tumors was more reflective of matching metastatic tumor biopsies than pre-chemotherapy samples. These findings support a portrait of modest ongoing genomic instability with respect to single-nucleotide variants induced by or selected for by chemotherapy in TNBCs.
High Expression of THY1 in Intestinal Gastric Cancer as a Key Factor in Tumor Biology: A Poor Prognosis-Independent Marker Related to the Epithelial–Mesenchymal Transition Profile
Authors: Rohan Paulo , dos Santos Everton Cruz , Abdelhay Eliana , Binato Renata
Published: December 2023

Gastric cancer (GC) is an important cancer-related death worldwide. Among its histological subtypes, intestinal gastric cancer (IGC) is the most common. A previous work showed that increased expression of the THY1 gene was associated with poor overall survival in IGC. Furthermore, it was shown that IGC tumor cells with high expression of THY1 have a greater capacity for tumorigenesis and metastasis in vitro. This study aimed to identify molecular differences between IGC with high and low expression of THY1. Using a feature selection method, a group of 35 genes were found to be the most informative gene set for THY1high IGC tumors. Through a classification model, these genes differentiate THY1high from THY1low tumors with 100% of accuracy both in the test subset and the independent test set. Additionally, this group of 35 genes correctly clustered 100% of the samples. An extensive validation of this potential molecular signature in multiple cohorts successfully segregated between THY1high and THY1low IGC tumors (>95%), proving to be independent of the gene expression quantification methodology. These genes are involved in central processes to tumor biology, such as the epithelial–mesenchymal transition (EMT) and remodeling of the tumor tissue composition. Moreover, patients with THY1high IGC demonstrated poor survival and a more advanced clinicopathological staging. Our findings revealed a molecular signature for IGC with high THY1 expression. This signature showed EMT and remodeling of the tumor tissue composition potentially related to the biology of IGC. Altogether, our results indicate that THY1high IGC tumors are a particular subset of tumors with a specific molecular and prognosis profile.
Children with Rare Nager Syndrome—Literature Review, Clinical and Physiotherapeutic Management
Authors: Marszałek-Kruk Bożena Anna , Myśliwiec Andrzej , Lipowicz Anna , Wolański Wojciech , Kulesa-Mrowiecka Małgorzata , Dowgierd Krzysztof
Published: December 2023

Nager syndrome is a rare human developmental disorder characterized by craniofacial defects including the downward slanting of the palpebral fissures, cleft palate, limb deformities, mandibular hypoplasia, hypoplasia or absence of thumbs, microretrognathia, and ankylosis of the temporomandibular joint. The prevalence is very rare and the literature describes only about a hundred cases of Nager syndrome. There is evidence of autosomal dominant and autosomal recessive inheritance for Nager syndrome, suggesting genetic heterogeneity. The majority of the described causes of Nager syndrome include pathogenic variants in the SF3B4 gene, which encodes a component of the spliceosome; therefore, the syndrome belongs to the spliceosomopathy group of diseases. The diagnosis is made on the basis of physical and radiological examination and detection of mutations in the SF3B4 gene. Due to the diversity of defects associated with Nager syndrome, patients require multidisciplinary, complex, and long-lasting treatment. Usually, it starts from birth until the age of twenty years. The surgical procedures vary over a patient’s lifetime and are related to the needed function. First, breathing and feeding must be facilitated; then, oral and facial clefts should be addressed, followed by correcting eyelid deformities and cheekbone reconstruction. In later age, a surgery of the nose and external ear is performed. Speech and hearing disorders require specialized logopedic treatment. A defect of the thumb is treated by transplanting a tendon and muscle or transferring the position of the index finger. In addition to surgery, in order to maximize a patient’s benefit and to reduce functional insufficiency, complementary treatments such as rehabilitation and physiotherapy are recommended. In our study, we describe eight patients of different ages with various cases of Nager syndrome. The aim of our work was to present the actual genetic knowledge on this disease and its treatment procedures.
The Efficacy of a Human-Ready miniMECP2 Gene Therapy in a Pre-Clinical Model of Rett Syndrome
Authors: Sadhu Chanchal , Lyons Christopher , Oh Jiyoung , Jagadeeswaran Indumathy , Gray Steven J. , Sinnett Sarah E.
Published: December 2023

Inactivating mutations and the duplication of methyl-CpG binding protein 2 (MeCP2), respectively, mediate Rett syndrome (RTT) and MECP2 duplication syndrome. These disorders underscore the conceptual dose-dependent risk posed by MECP2 gene therapy for mosaic RTT patients. Recently, a miRNA-Responsive Autoregulatory Element (miRARE) mitigated the dose-dependent toxicity posed by self-complementary adeno-associated viral vector serotype 9 (AAV9) miniMECP2 gene therapy (scAAV9/miniMECP2-myc) in mice. Here, we report an efficacy assessment for the human-ready version of this regulated gene therapy (TSHA-102) in male Mecp2−/y knockout (KO) mice after intracerebroventricular (ICV) administration at postnatal day 2 (P2) and after intrathecal (IT) administration at P7, P14 (±immunosuppression), and P28 (±immunosuppression). We also report qPCR studies on KO mice treated at P7-P35; protein analyses in KO mice treated at P38; and a survival safety study in female adult Mecp2−/+ mice. In KO mice, TSHA-102 improved respiration, weight, and survival across multiple doses and treatment ages. TSHA-102 significantly improved the front average stance and swing times relative to the front average stride time after P14 administration of the highest dose for that treatment age. Viral genomic DNA and miniMECP2 mRNA were present in the CNS. MiniMeCP2 protein expression was higher in the KO spinal cord compared to the brain. In female mice, TSHA-102 permitted survivals that were similar to those of vehicle-treated controls. In all, these pivotal data helped to support the regulatory approval to initiate a clinical trial for TSHA-102 in RTT patients (clinical trial identifier number NCT05606614).
Tetrad Effect of Rare Earth Element Fractionation in Zircon from the Pegmatite of the Adui Massif, Middle Urals
Authors: Levashova Ekaterina V. , Skublov Sergey G. , Zamyatin Dmitry A. , Li Qiuli , Levashov Dmitry S. , Li Xianhua
Published: December 2023

The zircon from the pegmatite of the Adui granitic massif displays the unique the rare earth element (REE) distribution spectrum with the tetrad effect in REE fractionation. The tetrad effect often occurs in granitoid rocks, but it is rarely encountered in minerals, e.g., zircon. Fluid saturated with volatiles, water and trace elements is a factor responsible for the tetrad effect in the zircon. The detailed isotopic-geochemical study of the zircon has revealed several zones differing in internal structure (in the back-scattered electron (BSE) image), composition and REE distribution. The zones indicate changes in the crystallization environment provoked by the evolution of the pegmatite-forming melt. They occur as the gradually growing changes in composition from the unaltered zones that are light-colored in BSE to the altered zones that are dark-colored in BSE. The unaltered zones are consistent in composition and geochemical features with magmatic zircons. The high content of trace (U, Th, REE) and volatile elements (F, Cl) in water suggests its crystallization from the fluid-saturated magmatic melt. The altered zircon zones occur as recrystallized zones with high content of non-formula elements (Y, Ca, Sr, Nb, P), a non-differentiated REE distribution spectrum and an absent Ce anomaly. These features are consistent with those of hydrothermal-metasomatic zircon.
An Enhanced Catalog of Repeating Earthquakes on the 1980 Irpinia Fault System, Southern Italy
Authors: Palo Mauro , Scotto di Uccio Francesco , Picozzi Matteo , Festa Gaetano
Published: December 2023

A pilot study employing a template-matching approach on the detection of repeating earthquakes (or repeaters) in a small volume of the Irpinia fault (Southern Italy) is presented here. A catalog of repeaters nucleating over about a decade with local magnitudes ranging between ML 0.4 and ML 1.7 is generated. The events nucleate at depths of about 11 km over a fault patch with a size of about 600 m. Assuming a constant stress drop for all repeaters, the yearly coseismic slip rate of the patch is estimated in the range of 1–4.8 mm with a mean value of about 3.4 mm, which is close to the fault slip rate estimated by geodetic approaches. Given the mechanical properties of the medium in the source region of the repeaters revealed by previous tomographic studies, and in particular the high Vp/Vs ratio (close to 1.95), it is postulated that these events are fluid triggered, consistently with the mechanism proposed for the generation of most of the microseismicity in the area. The encouraging outcomes of this study suggest to focus future measurement campaigns on this area and open perspectives to extend this approach to other segments of the Irpinia fault to reveal small-scale frictional properties in an area capable to generate M7 earthquakes.
Development of an Implementation Strategy Tailored to Deliver Evidence-Based and Person-Centred Nursing Care for Patients with Community-Acquired Pneumonia: An Intervention Mapping Approach
Authors: Eekholm Signe , Samuelson Karin , Ahlström Gerd , Lindhardt Tove
Published: December 2023

Community-acquired pneumonia is a serious public health problem, and more so in older patients, leading to high morbidity and mortality. However, this problem can be reduced by optimising in-hospital nursing care. Accordingly, this study describes a systematic process of designing and developing a tailored theory- and research-based implementation strategy that supports registered nurses (RNs) in delivering evidence-based and person-centred care for this patient population in a hospital setting. The implementation strategy was developed by completing the six steps of the Intervention Mapping framework: (1) developing a logic model of the problem and (2) a logic model of change by defining performance and change objectives, (3) designing implementation strategy interventions by selecting theory-based change methods, (4) planning the interventions and producing materials through a co-design approach, (5) developing a structured plan for adoption, maintenance and implementation and (6) developing an evaluation plan. This method can serve as a guide to (1) target behavioural and environmental barriers hindering the delivery of nursing care in local clinical practice, (2) support evidence uptake, (3) support RNs in the delivery of nursing care according to individual patient needs and thereby (4) optimise health-related patient outcomes.
Strength Training vs. Aerobic Training for Managing Pain and Physical Function in Patients with Knee Osteoarthritis: A Systematic Review and Meta-Analysis
Authors: Ceballos-Laita Luis , Lahuerta-Martín Silvia , Carrasco-Uribarren Andoni , Cabanillas-Barea Sara , Hernández-Lázaro Héctor , Pérez-Guillén Silvia , Jiménez-del-Barrio Sandra
Published: December 2023

(1) Background: Strength training (ST) and aerobic training (AT) are the most recommended interventions in patients with knee OA. These recommendations are supported by high-quality evidence, but it is still unknow whether one type of exercise is superior to the other. Thus, the aim was to investigate whether one type of exercise (ST or AT) is superior to the other for improving pain and physical function in patients with knee osteoarthritis. (2) Methods: A systematic review and meta-analysis was carried out following the PRISMA statement. The search strategy was conducted in PubMed, PEDro, Scopus, Web of Science and Cochrane Library databases. Randomized controlled trials comparing ST and AT on pain intensity and physical function in patients with knee osteoarthritis were included. Methodological quality and risk of bias were assessed with a PEDro scale and risk-of-bias tool, respectively. The certainty of evidence was evaluated using GRADE guidelines. (3) Results: Four studies (6 publications) were included. The qualitative and quantitative synthesis showed that ST produces no more improvement in pain intensity (SMD after intervention: 0.02; 95%CI: −0.15, 0.19; I2: 0%; three studies; 426 patients) and physical function (SMD after intervention: 0.07; 95%CI: −0.10, 0.24; I2: 0%; three studies; 426 patients) compared to AT in patients with knee osteoarthritis. The certainty of evidence was rated as very low. (4) Conclusions: Both type of exercises showed clinical benefits in people with knee osteoarthritis, but no differences between ST and AT were found.
Reconstructive Surgery of Pressure Injuries in Spinal Cord Injury/Disorder Patients: Retrospective Observational Study and Proposal of an Algorithm for the Flap Choice
Authors: Sgarzani Rossella , Rucci Paola , Landi Siriana , Battilana Micaela , Capirossi Rita , Aramini Beatrice , Negosanti Luca
Published: December 2023

Pressure injuries (PIs) are a common complication in patients with spinal cord injury/disorder (SCI/D), and deep PIs require surgical treatment consisting of wide debridement and adequate reconstruction. We conducted a retrospective observational study at a tertiary rehabilitation hospital for SCI/D in Italy with the aim of describing the incidence and associated risk factors of postoperative complications in individuals with SCI/D presenting with chronic deep PIs, treated with a specific flap selection algorithm based on the site of the defect, the presence of scars from previous surgeries, and the need to spare reconstructive options for possible future recurrences. Medical records of surgical procedures performed on SCI/D patients with fourth-degree PIs, according to NPUAP classification (National Pressure Ulcer Advisory Panel), between July 2011 and January 2018 were reviewed. A total of 434 surgical procedures for fourth-degree PIs in 375 SCI/D patients were analyzed. After a mean follow-up of 21 months (range 12–36), 59 PIs (13.6%) had minor complications, and 17 (3.9%) had major complications requiring reoperation. The sacral site and muscular and musculocutaneous flaps were significant risk factors for postoperative complications. Six patients (1.4%) had a recurrence. The choice of flap correlates with the outcome of decubitus reconstruction. Therefore, reconstructive planning should be based on established principles.
Breastfeeding vs. Formula Feeding and Maternal Sexuality among Polish Women: A Preliminary Report
Authors: Szablewska Anna Weronika , Michalik Anna , Czerwińska-Osipiak Agnieszka , Zdończyk Sebastian Artur , Śniadecki Marcin , Bukato Katarzyna , Kwiatkowska Wanda
Published: December 2023

Although postpartum sexual problems are common, there is a poor understanding of the underlying influencing factors and the impact of the infant feeding method on the mother’s sexual life. A cross-sectional control study was conducted with a group of 253 women during their postpartum period. This study aimed to investigate the effects of different infant feeding methods on female sexual life after childbirth. The study followed the STROBE guidelines for cross-sectional control analysis. The study design included a questionnaire characterizing sociodemographic, obstetric and breastfeeding variables and the PL-FSFI (Female Sexual Function Index). The authors collected the data in compliance with the CAWI (Computer-Assisted Web Interview) research methodology—an interview conducted via an Internet channel. Each respondent received and completed the survey provided to them via the same online link. This study included women in the postpartum period: 170 breastfeeding women (study group) and 83 formula-feeding women (control group). There were statistically significant difference between the groups that practiced different types of breastfeeding. Out of all the PL-FSFI-assessing domains, the highest average score for the whole group correlated with satisfaction and the lowest score correlated with lubrication use. Our findings indicate that women practicing only breastfeeding are more likely to develop sexual problems. In order to maintain sexual health and promote long-term breastfeeding, extensive and professional counseling is needed for couples about postpartum sexuality and the factors that affect it, such as breastfeeding.
Association of Basic Psychological Need Fulfillment and School Happiness with Obesity Levels and Intensity of Physical Activity during Physical Education Classes in South Korean Adolescents
Authors: Yun Ju-Seok , Lee Gyu-Il , Kim Bo-Ram
Published: December 2023

This study examined the relationship between basic psychological need fulfillment (BPNF) and school happiness in relation to the intensity of physical activity exhibited by adolescents during physical education (PE) classes and their obesity levels. We measured BPNF and school happiness using a questionnaire administered to 600 junior high school students. After exclusion, 532 questionnaires were analyzed. A 3D accelerometer (GT3X model) from Actigraph and the body mass index were used to measure physical activity intensity and obesity, respectively. The findings of this study indicate that BPNF and school happiness were significantly associated with physical activity intensity. Specifically, students who engaged in high-intensity physical activity exhibited higher levels of BPNF and school happiness. Additionally, BPNF and school happiness were not significantly related to obesity, whereas a significant relationship was observed between BPNF, school happiness, and the interaction between physical activity intensity and obesity level. This finding implies that adolescents with higher physical activity intensity and lower obesity reported higher levels of BPNF and school happiness. These findings suggest that physical activity intensity during PE classes significantly influences emotional development. Therefore, incorporating moderate-to-vigorous physical activity into PE may serve as a strategy for promoting emotional development in adolescents.
Effects of Simulated Visual Impairment Conditions on Movement and Anxiety during Gap Crossing
Authors: Uno Tadashi , Matsuo Taihei , Asano Masanari , Loh Ping Yeap
Published: December 2023

This study investigated the effects of visual conditions associated with progressive eye disease on movement patterns and anxiety levels during gap-crossing tasks. Notably, 15 healthy young adults performed crossover platforms with a 10 cm gap at three different heights, namely equal (0 cm), raised (+15 cm), and lowered (−15 cm) levels, under four vision conditions, namely normal or corrected eyesight, 10° tunnel vision, 5° tunnel vision, and 5° tunnel vision with 0.04 occlusion. Leg movements during gap crossing were analyzed using three-dimensional motion analysis. The results highlighted a distinct motion pattern in the trajectories of participants’ legs under the different visual conditions. Specifically, at the point where the gap-crossing movement began (D1), the normal or corrected eyesight conditions resulted in further separation between the steps compared with the other visual conditions. The highest point of the foot during movement (D2) did not differ between the visual conditions, except for the 0 cm step. Furthermore, anxiety levels, as quantified by the State-Trait Anxiety Inventory (STAI-S) questionnaire, were exacerbated under conditions of restricted visual information. In conclusion, visual impairments associated with progressive ocular diseases may perturb complex motor movement patterns, including those involved in gap-crossing tasks, with heightened anxiety potentially amplifying these disturbances.
Asymptomatic Autonomic Dysregulation after Recovery from Mild COVID-19 Infection Revealed by Analysis of Heart Rate Variability Responses to Task Load
Authors: Shinba Toshikazu , Shinba Yujiro , Shinba Shuntaro
Published: December 2023

(1) Background: The coronavirus disease 2019 (COVID-19) infection is often followed by various complications, which can cause disturbances in daily life after recovery from the infectious state, although etiological mechanisms are not fully elucidated. Previous studies have indicated that autonomic dysregulation is an underlying factor, and it is of interest to clarify whether autonomic dysregulation is also present in the asymptomatic subjects after COVID-19 infection (post-COVID-19) for early detection of post-COVID-19 complications. (2) Methods: In the present study, autonomic activity was assessed using heart rate variability (HRV) analysis in the workers who recovered from mild COVID-19 infection (n = 39). They took a leave of absence for an average of 11.9 days and returned to the original work without complications. HRV was measured after an average of 9.3 days from return. High-frequency (HF) and low-frequency (LF) HRV parameters and heart rate (HR) were recorded during a three-behavioral-state paradigm of approximately 5 min length composed of initial rest, task load, and post-task rest periods and were compared with the data of the workers without the history of COVID-19 infection (normal, n = 38). (3) Results: The HRV and HR scores at the initial rest in the post-COVID-19 subjects showed no difference from those in the control. It is found that the post-COVID-19 subjects exhibited an attenuation of LF/HF increment during the task load and an excessive increase of HF together with a decrease of LF, LF/HF and HR during the post-task rest period in comparison with the initial rest scores. (4) Conclusions: These abnormalities are evaluated as asymptomatic autonomic dysregulation in response to task load, are frequently present after COVID-19 infection, and could be related to the generation of complications.
Effects of Dynamic Stretching Combined with Manual Therapy on Pain, ROM, Function, and Quality of Life of Adhesive Capsulitis
Authors: Choi Jeong-Min , Cho Eun-Young , Lee Byoung-Hee
Published: December 2023

This study was conducted to evaluate the effects of dynamic stretching combined with manual therapy on pain, range of motion, function, and quality of life in patients with adhesive capsulitis. The participants were randomly divided into two groups: the dynamic stretching combined with manual therapy (DSMT) group (n = 17) and the static stretching combined with manual therapy (SSMT) group (n = 17). Both groups received manual therapy for 10 min and two sessions per week for 4 weeks. The DSMT group also performed additional dynamic stretching for 20 min per session, two sessions per week for 4 weeks. The SSMT group practiced additional static stretching for 20 min per session, two sessions per week for 4 weeks. The pain, ROM, function, and quality of life were measured and evaluated before and after treatment. There were significant improvements in the outcomes of pain, flexion and abduction of shoulder ROM, Shoulder Pain and Disability Index (SPADI), and the physical component score and mental component score of the Short Form-36 (SF-36) in both groups. Additionally, the external and internal rotation of the shoulder ROM and the SF-36 general health factor increased significantly more in the A group (DSMT group) compared to the B group (SSMT). In conclusion, dynamic stretching plus manual therapy offers the same results as static stretching plus manual therapy, but with additional improvement in internal and external rotation.
Microbial Allies in Agriculture: Harnessing Plant Growth-Promoting Microorganisms as Guardians against Biotic and Abiotic Stresses
Authors: Teiba Islam I. , El-Bilawy Emad H. , Elsheery Nabil I. , Rastogi Anshu
Published: December 2023

Plants face many biological and non-biological challenges throughout their life cycle, from seed to harvest. These challenges have recently increased due to climate changes. Strategies for confronting different types of stresses depend on the type of stress, the cultivated plant, climatic conditions, soil characteristics, water variables, cost, and management system. Chemical methods (fertilizers and pesticides) have been widely used to manage abiotic and biotic stresses, but they raise concerns about environmental contamination, toxic residues, and the development of resistant pathogens. Eco-friendly strategies have recently become one of the most important approaches to obtaining high-quality and quantitative plant-based products. Microbial inoculants, such as plant growth-promoting microorganisms (PGPM), offer a sustainable alternative to chemical methods. PGPM can augment plant growth and nutrition, improve plant tolerance to abiotic stresses, and reduce the growth of certain pathogens. They employ a variety of mechanisms to alleviate stressors and boost plant resilience, including nutrient assimilation, production of metabolites, and activation of systemic resistance. This review aims to elucidate the impact of PGPM, with a particular focus on plant growth-promoting bacteria (PGPB), and their mechanisms of action on plants under varying stressors, while also identifying areas for further research in both PGPB and other non-bacterial organisms.
Response of Metabolites in Cymbopogon distans Leaves to Water Addition in Karst Areas during Different Seasons
Authors: Huang Aiwei , Jing Hongxia , Umair Muhammad , Du Hongmei
Published: December 2023

Climate change could influence the plant response to drought stress in karst environments. However, fewer related studies have been reported. This study examined the impact of artificial water addition on the accumulation of metabolites of Cymbopogon distans with a non-targeted metabolomics approach during both the dry and wet seasons. Three water treatment gradients (CK, T1, and T2, indicating 0%, +20%, and +40% relative to the average monthly precipitation, respectively) were chosen. The findings of our study indicate that the levels of primary metabolites were higher in the leaves of C. distans during the dry season compared to the rainy season. In addition, the presence of water did not have a substantial impact on the composition and functionality of metabolites between the wet and drought seasons. The contents of some lipids were greater during the dry season, while others were greater during the wet season. During the dry season, the contents of FA, DG, MGDG, SQDG, TG, and PR decreased with water addition. Our findings demonstrated that artificial water addition might have a greater impact on metabolite accumulation during the dry season in drought-tolerant species in karst areas. Due to the buildup of certain metabolites, they exhibit clear drought resistance. At the same time, water addition during the dry season will also cause a certain stress, affecting the adaptability of plants. These findings have significant ramifications for the management and choice of species in various sea seasons in karst regions.
Comparative Proteomics Analysis of Primulina serrulata Leaves Reveals New Insight into the Formation of White Veins
Authors: Dou Quan-Li , Xie Da-Jun , Deng Tan , Chen Mo-Fang , Qian Zheng-Min , Wang Shuang-Shuang , Zhang Ren-Bo
Published: December 2023

Primulina serrulata is a valuable ornamental herb with rosette leaves and vibrant flowers. Some leaves of this species exhibit a bright and distinct white color along the upper veins, enhancing their ornamental value, while others are less white or entirely green. This variation is observed in adult leaves from natural habitats and among young leaves from seedlings grown in the laboratory. TMT-labeled proteomics technology was used to study the protein-level biogenesis of white-veined (WV) P. serrulata leaves. Our objective was to offer novel insight into the breeding of WV plants. Chlorophyll (Chl) content was significantly lower in the WV group than in the control group. Out of 6261 proteins identified, a mere 69 met the criteria for differentially expressed proteins (DEPs) after stringent screening for subsequent analyses. Among these DEPs, there were 44 proteins that exhibited downregulation and 25 that were upregulated in the WV plants. Some DEPs associated with chloroplasts and Chl biosynthesis were downregulated, leading to the absence of green coloration. Concurrently, Gene Ontology enrichment analysis further emphasized an insufficiency of magnesium, the key element in Chl biosynthesis. Many DEPs associated with abiotic or biotic stressors were downregulated, suggesting an overall weakening of stress resistance with certain compensatory mechanisms. Similarly, many DEPs related to modifying biomacromolecules were downregulated, possibly affected by the decrease in proteins involved in photosynthesis and stress resistance. Some DEPs containing iron were upregulated, indicating that iron is mainly used to synthesize heme and ferritin rather than Chl. Additionally, several DEPs related to sulfur or sulfate were upregulated, suggesting strengthened respiration. Expansin-A4 and pectinesterase were upregulated, coinciding with the emergence of a rough and bright surface in the white area of leaves, indicative of the elongation and gelation processes in the cell walls. These findings provide new insight for future studies to explore the mechanism of color formation in WV leaves.
Artificial Polyploidization Enhances Morphological, Physiological, and Biological Characteristics in Melothria scabra Naudin
Authors: Fernández-Cusimamani Eloy , Bharati Rohit , Javůrková Tereza Anna , Škvorová Petra , Paznocht Luboš , Kotikova Zora , Kouřimská Lenka , Orsák Matyáš
Published: December 2023

Cucamelon (Meltric scabra Naudin, Cucurbitaceae) is a climbing, herbaceous perennial plant with various culinary and medicinal uses. The current study aimed to develop novel autopolyploid genotypes of M. scabra through in vitro polyploidization and assess their morphological and phytochemical characteristics. For polyploid induction, oryzalin was employed as an antimitotic agent, and it was applied at various concentrations (40, 60, and 80 μM) and durations (24 and 48 h). Flow cytometry analysis confirmed the successful induction of polyploids, with polyploidization efficiency ranging from 2.5% to 15%. From a total of 240 treated plants, a total of 20 autotetraploid plants were obtained. The obtained polyploid and control diploid genotypes were cultivated under greenhouse conditions. Further, the plants were transferred to field conditions, and the leaves, flowers, and fruits were harvested to be evaluated for the morphological, biochemical, and biological activity variations among the obtained genotypes. Morphological comparisons between diploid and autotetraploid plants revealed significant differences in flower characteristics, fruit attributes, and leaf morphology. Nutritional evaluation demonstrated enhancement of key parameters in the induced polyploids compared to the diploid plants, including glucose, fructose, carotenoid, polyphenol, and antioxidant contents, highlighting the potential impact of polyploidization on these traits. The results from this study highlight the potential of artificial chromosome doubling as an effective breeding strategy in M. scabra and related plant species.
Child Maltreatment Types by Age: Implications for Prevention
Authors: Guastaferro Kate , Shipe Stacey L.
Published: December 2023

Child maltreatment is a global public health issue known to affect an average of 600,000 U.S. children of all ages (0–18 years old) annually. However, a preponderance of preventive programs target children on the younger end of the spectrum, specifically those aged 0–5. Annual reports of the prevalence of maltreatment provide opportunities to analyze trends, but in 2009, these reports stopped reporting the ages of victims for each type of maltreatment (i.e., neglect, physical abuse, emotional abuse, and sexual abuse). This omission limits the ability to match (or design) prevention programs responsive to the ages of those at greatest risk. Using data from the National Child Abuse and Neglect Data System (NCANDS) from 2011–2020, this secondary data analysis describes trends for four types of maltreatment by age from an epidemiological perspective. Implications for practice (i.e., prevention) and policy are presented. The findings of this study offer the first step in what is hoped to be a line of research that seeks to identify, match, and/or develop evidence-based programs to prevent child maltreatment among the populations at highest risk.
Digital Mental Health Interventions: Differences in Diet Culture Intervention Framing
Authors: Fitterman-Harris Hannah F. , Davis Gabrielle G. , Bedard Samantha P. , Cusack Claire E. , Levinson Cheri A.
Published: December 2023

Diet culture is a societal norm that ranks thin bodies as superior to other body types and has been associated with negative outcomes, such as eating disorders. Wellness has evolved into a term that is often used to promote diet culture messages. One possible way to combat diet culture is through single-session, digital mental health interventions (DMHIs), which allow for increased access to brief public health treatments. The framing of DMHIs is critical to ensure that the target population is reached. Participants (N = 397) were enrolled in a single-session DMHI, which was framed as either a Diet Culture Intervention (n = 201) or a Wellness Resource (n = 196). Baseline group differences in eating disorder pathology, body image, weight stigma concerns, fat acceptance, and demographic characteristics were analyzed. Across groups, participants reported moderately high eating disorder pathology, low-to-moderate levels of body dissatisfaction, moderate levels of fat acceptance, and either very low or very high weight stigma concerns. Participants in the Diet Culture Intervention group reported higher levels of fat acceptance than those in the Wellness Resource group (p < 0.001). No other framing group differences were identified, though post hoc analyses revealed differences based on recruitment source (i.e., social media versus undergraduate research portal). This study found that framing a DMHI as targeting diet culture or as a Wellness Resource can result in the successful recruitment of individuals at risk of disordered eating. Framing a DMHI as a Wellness Resource may increase recruitment of individuals with low levels of fat acceptance, which may be particularly important for dismantling diet culture, disordered eating, and weight stigma concerns. Future research should assess DMHI framing in other populations, such as men and adolescents.
Climate Stressors and Physiological Dysregulations: Mechanistic Connections to Pathologies
Authors: Heidari Hajar , Lawrence David A.
Published: December 2023

This review delves into the complex relationship between environmental factors, their mechanistic cellular and molecular effects, and their significant impact on human health. Climate change is fueled by industrialization and the emission of greenhouse gases and leads to a range of effects, such as the redistribution of disease vectors, higher risks of disease transmission, and shifts in disease patterns. Rising temperatures pose risks to both food supplies and respiratory health. The hypothesis addressed is that environmental stressors including a spectrum of chemical and pathogen exposures as well as physical and psychological influences collectively impact genetics, metabolism, and cellular functions affecting physical and mental health. The objective is to report the mechanistic associations linking environment and health. As environmental stressors intensify, a surge in health conditions, spanning from allergies to neurodegenerative diseases, becomes evident; however, linkage to genetic-altered proteomics is more hidden. Investigations positing that environmental stressors cause mitochondrial dysfunction, metabolic syndrome, and oxidative stress, which affect missense variants and neuro- and immuno-disorders, are reported. These disruptions to homeostasis with dyslipidemia and misfolded and aggregated proteins increase susceptibility to cancers, infections, and autoimmune diseases. Proposed interventions, such as vitamin B supplements and antioxidants, target oxidative stress and may aid mitochondrial respiration and immune balance. The mechanistic interconnections of environmental stressors and disruptions in health need to be unraveled to develop strategies to protect public health.