bluuebunny/crossref_metadata_2025 huggingface dataset:
Path Planning of Multiple Unmanned Aerial Vehicles Covering Multiple Regions Based on Minimum Consumption Ratio
Authors: Chen Jian , Zhang Ruikang , Zhao Hongqiang , Li Jiejie , He Jilin
Published: January 2023

Investigating the path planning of multiple unmanned aerial vehicles (UAVs) covering multiple regions, this work proposes an effective heuristic method of region coverage path planning to reduce the complexity of the problem. The proposed method decomposes the solution process into two stages. First of all, the two most important parameters affecting the performance of UAV missions were considered, namely, the flying speed and the scan width. According to these two parameters of UAVs, a new multi-regional allocation scheme based on the minimum consumption ratio was proposed. With this allocation scheme, the coverage task allocation and path pre-planning of UAVs were obtained. Then, the UAVs’ trajectory routes were optimized based on the dynamic planning algorithm to reduce the time consumption of UAVs on the transfer path between regions. The method was evaluated with numerical experiments. The results showed that the proposed method can effectively solve the path planning problem of multiple UAVs covering multiple regions. Compared with an advanced algorithm, the time consumption for homogeneous and heterogenous UAV performance was reduced by 5.1% and 3%, respectively.
Water Budget of Urban Turf Field and Optimal Irrigation Schedule Simulation in an Ecotone between Semi-Humid and Semi-Arid Regions, Northern China
Authors: Zhang Hongjuan , Wang Jianjun , Liu Mengzhu , Shen Yanjun , Pei Hongwei
Published: January 2023

Water security in the ecotone between semi-humid and semi-arid regions (EHA) is very vulnerable and sensitive to climate change and human interferences. Urban turf irrigation is a primary consumer of urban water resources in the EHA, which places huge pressures on water security by substantial irrigated water use due to the expansion of urban turf planting. Based on a 2-year (2020–2021) turf experiment in Zhangjiakou City, a typical water-deficit city in the EHA of northern China, the water budget for turf was measured and analyzed. Furthermore, the Root Zone Water Quality Model (RZWQM2) was employed to evaluate the optimal irrigation scheme for turf. The results showed that the average volumetric water content in the 0–40 cm soil layer was maintained above 23% in 2020–2021. The evapotranspiration in growth period of turf accounted for more than 70% of the annual evapotranspiration, and the deep seepage in turf soil accounted for 49.67% and 60.28% of the total precipitation and irrigation in 2020 and 2021, respectively, during the vigorous growth period of the turf from May to September. The calibrated RZWQM2 showed a robust ability to simulate the water changes in turf. The d-values (consistency index) between the simulated and observed volumetric water contents and evapotranspiration were both greater than 0.90. In the aspects of irrigation scenarios, the T60%-12 scenario (TA-B, where A is 100%, 80%, 60% or 40% of the total irrigation amount and B is the number of irrigation events corresponding to A) was determined as the best irrigation schedule in our study area because of lower evapotranspiration, seepage and higher turf soil water storage under this irrigation scenario, also resulting from the comparison of different irrigation scenarios using the entropy-weight-TOPSIS method. In such an optimal scenario, T60%-12 irrigation treatment reduced the irrigated water requirement of turf by 40% (142.06 mm) and the seepage amount by 28.07% (39.05 mm), and had the lowest negative impacts on the turf growth.
Improvement of Tomato Fruit Quality and Soil Nutrients through Foliar Spraying Fulvic Acid under Stress of Copper and Cadmium
Authors: Shi Xiaodi , Zhang Lingfei , Li Zehan , Xiao Xiangyang , Zhan Nanbiao , Cui Xiumin
Published: January 2023

Fulvic acid (FA), the essence and most active component in humus, is widely used as a fertilizer synergistic agent and for soil improvement. As a synergist, FA can not only highly chelate microelements, but also play a key role as a growth promoter. Due to the small molecular weight and high solubility, FA is usually used by foliar spraying in vegetable production, yet the effect on fruit quality and nutrient absorption is still unclear. Here, ‘Jinpengdashuai’ tomatoes were used to investigate the effects of spraying FA on tomato fruit quality and soil Cu and Cd availability under stress of Cu and Cd by pot experiments. The results showed that the 1000 mg·L−1 FA could significantly improve the biomass of tomato plants to some extents under different stresses of Cu and Cd. After spraying FA, the Cu and Cd content in different organs and the whole accumulation decreased; meanwhile, the transport efficiency of Cu and Cd was also reduced to some degree. The drops of FA significantly changed the chemical form of Cu and Cd in 0~10 cm soil, from the easily absorbed water soluble (or ion) form into the insoluble form, hard to absorb. The Cu content in the residual state increased by 93.8% and 172.5%, respectively, under single and compound stress, and the Cd content in the residual state increased by 16.7% and 58.6%. Foliar spraying FA could promote the absorption and transport of essential nutrients such as nitrogen, phosphorus, potassium, calcium, magnesium and zinc, and maintain the nutrient balance, which alleviates the inhibition of normal metabolism by Cu and Cd stress, to a certain extent. More distinctly, Vc, total sugar and lycopene increased by 11.4~45.9%, 19.2~48.5% and 30.9~84.5%, respectively, indicating that foliar spraying FA could improve the appearance and flavor quality of tomato fruits under stress of Cu and Cd.
Interactions and Regulatory Functions of Phenolics in Soil-Plant-Climate Nexus
Authors: Misra Deblina , Dutta Writuparna , Jha Gaurav , Ray Puja
Published: January 2023

Phenols are major compounds produced by plant species as a peripheral stimulus or as a regulatory defense mechanism under different environmental biotic stresses. These secondary metabolites are generated from shikimic and acetic acid metabolic pathways. The aromatic benzene ring compound plays an important role in plant development, especially in the defense forefront. They provide structural integrity and support to the plants. Phenolic phytoalexins released by pathogen/arthropod-attacked or wounded plants nullify or repel organisms for the advantage of the host. The allelopathic potential of phenolic compounds is observed in both natural and managed ecosystems. The global impacts of climatic variabilities such as drought, increased carbon dioxide, or greenhouse gas emissions alter the quantitative response of plant phenols. This review primarily discusses the different aspects of phenolic interactions concerning health, antioxidant properties, and insect-plant interaction as a nexus of soil and plant relations in response to variable climatic conditions.
A Preliminary Report of Plastic Ingestion by Hawksbill and Green Turtles in the Saudi Arabian Red Sea
Authors: Tanabe Lyndsey K. , Cochran Jesse E. M. , Hardenstine Royale S. , Scott Kirsty , Berumen Michael L.
Published: January 2023

(1) Background: Plastic pollution is a major environmental concern confronting marine animals. Sea turtles are considered a bio-indicator of plastic pollution, but there is little information regarding plastic ingestion by turtles in the Red Sea. With large-scale development projects being built along the Saudi Arabian coast, it is important to have a baseline for plastic ingestion before construction is complete. (2) Methods: Ten deceased sea turtles (four hawksbill and six green turtles) were collected along the Saudi Arabian coastline. Necropsies were conducted, and the entire gastrointestinal tracts were extracted and the contents were passed through a 1 mm mesh sieve. (3) Results: We found that 40% of the turtles in this study had ingested plastics. Thread-like plastics were the most common plastic category, and multi-colored was the most prevalent color category. (4) Conclusions: Monitoring of the plastic ingestion by marine megafauna should be conducted as a long-term assessment of the developments’ impacts. Additionally, conservation efforts should be focused on removing plastics (namely ghost nests and fishing lines) from the reefs and reducing the amount of plastic entering the sea.
miR-202-5p Inhibits Lipid Metabolism and Steroidogenesis of Goose Hierarchical Granulosa Cells by Targeting ACSL3
Authors: Ran Mingxia , Hu Shenqiang , Ouyang Qingyuan , Xie Hengli , Zhang Xi , Lin Yueyue , Li Xuejian , Hu Jiwei , Li Liang , He Hua , Liu Hehe , Wang Jiwen
Published: January 2023

miRNAs are critical for steroidogenesis in granulosa cells (GCs) during ovarian follicular development. We have previously shown that miR-202-5p displays a stage-dependent expression pattern in GCs from goose follicles of different sizes, suggesting that this miRNA could be involved in the regulation of the functions of goose GCs; therefore, in this study, the effects of miR-202-5p on lipid metabolism and steroidogenesis in goose hierarchical follicular GCs (hGCs), as well as its mechanisms of action, were evaluated. Oil Red O staining and analyses of intracellular cholesterol and triglyceride contents showed that the overexpression of miR-202-5p significantly inhibited lipid deposition in hGCs; additionally, miR-202-5p significantly inhibited progesterone secretion in hGCs. A bioinformatics analysis and luciferase reporter assay indicated that Acyl-CoA synthetase long-chain family member 3 (ACSL3), which activates long-chain fatty acids for the synthesis of cellular lipids, is a potential target of miR-202-5p. ACSL3 silencing inhibited lipid deposition and estrogen secretion in hGCs. These data suggest that miR-202-5p exerts inhibitory effects on lipid deposition and steroidogenesis in goose hGCs by targeting the ACSL3 gene.
Microbiological Quality of Raw Donkey Milk from Serbia and Its Antibacterial Properties at Pre-Cooling Temperature
Authors: Šarić Ljubiša , Premović Tamara , Šarić Bojana , Čabarkapa Ivana , Todorić Olja , Miljanić Jelena , Lazarević Jasmina , Karabasil Nedjeljko
Published: January 2023

The aim of this study was to examine the microbiological quality of raw donkey milk of an indigenous Serbian breed as well as the changes in the microbial populations during storage at 4 °C. In addition, antibacterial activity of donkey milk against E. coli, L. monocytogenes and S. aureus at 15 °C as well as the content of the two main antibacterial proteins lysozyme and lactoferrin were investigated. Microbiological examination of 137 individual milk samples collected over a period of 21 months showed good microbiological quality since foodborne pathogens such as Salmonella spp. and L. monocytogenes were not detected in any of the analyzed samples, while the number of E. coli, Enterobacteriaceae, total coliform bacteria, sulfite-reducing Clostridia and aerobic sporogenic bacteria was below the limit of quantification (<1 cfu mL−1). During the six-days storage at 4 °C, total bacterial counts and the counts of lactic acid bacteria remained at the initial level while pathogenic bacteria were not detected. The strongest antibacterial activity of the tested milk was observed against E. coli, while S. aureus was the least sensitive to milk antibacterial compounds. Although further research is needed to fully elucidate the antibacterial mechanism and synergistic activity of different compounds in donkey milk, the high content lysozyme (2.63 ± 0.03 g L−1) and lactoferrin (15.48 mg L−1) observed in tested milk could contribute to its strong antibacterial activity and extension of the storage period during which it can be safely consumed.
Restoring Activity of Milk Thistle (Silybum marianum L.) on Serum Biochemical Parameters, Oxidative Status, Immunity, and Performance in Poultry and Other Animal Species, Poisoned by Mycotoxins: A Review
Authors: Guerrini Alessandro , Tedesco Doriana Eurosia Angela
Published: January 2023

Grains are major farm animals’ diet ingredients, and one of the main concerns is when are mycotoxin (MyT) contaminated, compromising animals’ health, performance, and product safety. Among the natural phytocompounds that are being used to prevent MyT damage, silymarin (SIL), an extract from the seed of the milk thistle (MT) is a suitable candidate. This review aims to examine the scientific evidence concerning the anti-MyT toxicity effects of MT/SIL in poultry and livestock. In vitro and in vivo studies (n = 27) showed that MT whole plant, seed, or SIL-standardized extract had positive effects on animal health, performance, and restoring the hepatic activity, with a reduction of organ lesions caused by MyT. Furthermore, showed utility for combating MyT-immunodepression, improving intestinal health, and limiting the excretion of toxins residues in food of animal origin, although in some cases, MT/SIL supplementation does not produce appreciable effects. The use of MT in animal nutrition can be useful since the bioactive compounds, also if present in variable amounts, can help the animals to counteract the effects of MyT. The use of the phytoextract of SIL, due to its cost, can be useful if it reported the specific bioactive compounds, recognize for their pharmacological activities.
Diet Composition and Using Probiotics or Symbiotics Can Modify the Urinary and Faecal Nitrogen Ratio of Broiler Chicken’s Excreta and Also the Dynamics of In Vitro Ammonia Emission
Authors: Such Nikoletta , Mezőlaki Ákos , Rawash Mohamed Ali , Tewelde Kesete Goitom , Pál László , Wágner László , Schermann Kornél , Poór Judit , Dublecz Károly
Published: January 2023

The objective of this research was to determine whether diet composition, or adding probiotic or symbiotic feed additives to broiler diets can modify the N composition of the excreta and the dynamics of ammonia volatilization from the manure. A total of 574 one-day-old Ross 308 broiler chickens were fed four different diets. The treatments included a corn and soybean meal-based control diets (C), wheat-based and wheat bran containing diets (W), a multi-strain probiotic treatment (Broilact®; Br), and a symbiotic additive containing Bacillus subtilis, inulin, and Saccharomices cerevisiae (Sy). Feeding the wheat-based diet significantly improved the weight gain and FCR of chickens. Treatment W also significantly increased the dry matter content of the excreta compared with the probiotic and symbiotic treatments. Both Br and Sy tended to decrease the amount of excreted uric acid, which is the main substrate of ammonia. Treatment Sy reduced the urinary N ratio of the excreta in comparison with treatment W. The symbiotic additive resulted in significantly higher ammonia emission in the first two hours. On the other hand, the dynamics of the emission was slow at the beginning and increased steeply after 15 h when the wheat-based diets were fed. Based on our results, the wheat-based diets, containing soluble arabinoxylans, and the symbiotic treatments of broiler diets have an impact on the urinary and faecal nitrogen composition of the excreta, and also on the dynamics of ammonia release from the manure.
Modeling the Impact of Liquid Polymers on Concrete Stability in Terms of a Slump and Compressive Strength
Authors: Mohammed Ahmed Salih , Emad Wael , Sarwar Qadir Warzer , Kurda Rawaz , Ghafor Kawan , Kadhim Faris Raed
Published: January 2023

It is generally known that the two most crucial elements of concrete that depend on the slump value of the mixture are workability and compressive strength. In addition, slump retention is more delicate than the commonly used slump value since it reflects the concrete mixture’s durability for usage in civil engineering applications. In this study, the effect of three water-reducer additives was tested on concrete’s workability and compressive strength from 1 day to 28 days of curing. The slump of the concrete was measured at the time of adding water to the mix and after 30 min of adding water. This study employed 0–1.5% (%wt) water-reducer additives. The original ratio between water and cement (wc) was 0.65, 0.6, and 0.56 for mixtures incorporating 300, 350, and 400 kg of cement. It was lowered to 0.3 by adding water-reducer additives based on the additives type and cement content. Depending on the kind and amount of water-reducer additives, w/c, gravel content, sand content, crushed content, and curing age, adding water-reducer additives to the concrete increased its compressive strength by 8% to 186%. When polymers were added to the concrete, they formed a fiber net (netting) that reduced the space between the cement particles. As a result, joining the cement particles quickly enhanced the fresh concrete’s viscosity and the hardened concrete’s compressive strength. The study aims to establish mathematical models (nonlinear and M5P models) to predict the concrete compressive strength when containing water-reducer additives for construction projects without theoretical restrictions and investigate the impact of mix proportion on concrete compressive strength. A total of 483 concrete samples modified with 3 water-reducer additives were examined, evaluated, and modeled for this study.
Predictor–Corrector Scheme for Electrical Magnetohydrodynamic (MHD) Casson Nanofluid Flow: A Computational Study
Authors: Nawaz Yasir , Arif Muhammad Shoaib , Abodayeh Kamaleldin
Published: January 2023

The novelty of this paper is to propose a numerical method for solving ordinary differential equations of the first order that include both linear and nonlinear terms (ODEs). The method is constructed in two stages, which may be called predictor and corrector stages. The predictor stage uses the dependent variable’s first- and second-order derivative in the given differential equation. In literature, most predictor–corrector schemes utilize the first-order derivative of the dependent variable. The stability region of the method is found for linear scalar first-order ODEs. In addition, a mathematical model for boundary layer flow over the sheet is modified with electrical and magnetic effects. The model’s governing equations are expressed in partial differential equations (PDEs), and their corresponding dimensionless ODE form is solved with the proposed scheme. A shooting method is adopted to overcome the deficiency of the scheme for solving only first-order boundary value ODEs. An iterative approach is also considered because the proposed scheme combines explicit and implicit concepts. The method is also compared with an existing method, producing faster convergence than an existing one. The obtained results show that the velocity profile escalates by rising electric variables. The findings provided in this study can serve as a helpful guide for investigations into fluid flow in closed-off industrial settings in the future.
Base Isolation Compared to Capacity Design for Long Corner Periods and Pulse-Type Seismic Records
Authors: Köber Dietlinde , Weber Felix , Lozincă Eugen , Popa Viorel
Published: January 2023

Southern Romania is a geographic region with alluvial deposits. This soil type leads to rather long corner periods and provides as a particularity of the response spectrum an enlarged plateau. These conditions produce large displacement demands. Moreover, pulse-type ground acceleration records make this seismic area more unique. Research on the seismic behaviour of structures built under such unusual conditions is limited and Romanian engineers are not confident to apply alternative solutions such as base isolation. Although capacity design is still the regular design method applied in Romania, modern base isolation solutions may overcome the large displacement demand expectation produced by seismic events and fulfil immediate occupancy requirements. This study presents the seismic performance of an existing hospital from Bucharest, for which two seismic design solutions were applied: (i) classical approach based on capacity design and (ii) base isolation. Both approaches are compared in terms of drift, acceleration and base shear values. Static as well as non-linear dynamic analysis methods were applied.
Cuffless Blood Pressure Estimation with Confidence Intervals using Hybrid Feature Selection and Decision Based on Gaussian Process
Authors: Lee Soojeong , Joshi Gyanendra Prasad , Shrestha Anish Prasad , Son Chang-Hwan , Lee Gangseong
Published: January 2023

Cuffless blood pressure (BP) monitoring is crucial for patients with cardiovascular disease and hypertension. However, conventional BP monitors provide only single-point estimates without confidence intervals. Therefore, the statistical variability in the estimates is indistinguishable from the intrinsic variability caused by physiological processes. This study introduced a novel method for improving the reliability of BP and confidence intervals (CIs) estimations using a hybrid feature selection and decision method based on a Gaussian process. F-test and robust neighbor component analysis were applied as feature selection methods for obtaining a set of highly weighted features to estimate accurate BP and CIs. Akaike’s information criterion algorithm was used to select the best feature subset. The performance of the proposed algorithm was confirmed through experiments. Comparisons with conventional algorithms indicated that the proposed algorithm provided the most accurate BP and CIs estimates. To the best of the authors’ knowledge, the proposed method is currently the only one that provides highly reliable BP and CIs estimates. Therefore, the proposed algorithm may be robust for concurrently estimating BP and CIs.
Numerical Gas–Liquid Two-Phase Flow Regime Identification in a Horizontal Pipe Using Dynamic Pressure Data
Authors: Khan Umair , Pao William , Sallih Nabihah
Published: January 2023

Gas–liquid two-phase flow is very common in industrial pipelines. Flow regime identification is the first step to design, analyze, and operate the gas–liquid system successfully. The purpose of this study is to develop a methodology for identification of a two-phase flow regime using post signal processing techniques, namely Fast Fourier Transform (FFT) and Probabilistic Density Function (PDF). Three different flow regimes were simulated in a 6 m horizontal pipe with a 0.050 m inner diameter. A Level-Set (LS) method coupled with Volume of Fluid (VOF) method is used to model the air–water interface. After validation of the numerical method, dynamic pressure readings were collected with the intent to identify the associated flow regimes by post-processing of these signals. It was concluded that dynamic pressure signals of different flow regimes show different characteristics (like dominant frequency, FFT amplitude, PDF location and PDF magnitude) in the time and frequency domains. These characteristics can be potentially used as differentiating factors to distinguish different flow regimes. This research is limited to stratified, slug, and annular flow in the horizontal pipe. This paper uses a new approach to identify the flow regime in a horizontal pipe by Fast Fourier Transform and Probability Density Function of dynamic pressure readings obtained by using numerical simulation.
Changes in Joint Power and Energetics during a Sport-Specific Jumping Fatigue Protocol
Authors: Wannop John , Schrier Nicole , Wolter Marie-Louise , Madden Ryan , Barrons Zach , Stefanyshyn Darren
Published: January 2023

Kinematic and kinetic changes in the lower extremities occur as an athlete becomes fatigued during vertical jumping; however, the specifics of these changes are not well-understood. Therefore, the purpose of this study was to quantify the influence of a sport-specific, vertical jumping fatigue protocol on the biomechanics of the ankle, knee, and hip joint. Twenty male varsity athletes performed repetitive standing countermovement squat jumps every 20 s until fatigued (vertical jump and reach height decreased to 88% of their maximum height for three consecutive jumps). The kinematics and kinetics of their lower extremities (ankle, knee, and hip) were quantified, and the ankle, knee, and hip joint’s moments, angular velocity, and joint power were compared. The participants performed an average of 175 jumps before they were classified as being fatigued. When they became fatigued, the peak power of the ankle and hip joints were substantially reduced due to a decrease in the angular velocity at both joints. Ankle and hip joint moments were unchanged. Peak power at the knee joint was also unchanged over the course of the jumping protocol. To maintain vertical jumping performance over the course of a game or to delay the influence of fatigue, training should be targeted at maintaining the angular velocity of the ankle and hip joints.
Large Field-Size Elliptic Curve Processor for Area-Constrained Applications
Authors: Rashid Muhammad , Sonbul Omar S. , Zia Muhammad Yousuf Irfan , Kafi Nadeem , Sinky Mohammed H. , Arif Muhammad
Published: January 2023

This article has proposed an efficient area-optimized elliptic curve cryptographic processor architecture over GF(2409) and GF(2571). The proposed architecture employs Lopez-Dahab projective point arithmetic operations. To do this, a hybrid Karatsuba multiplier of 4-split polynomials is proposed. The proposed multiplier uses general Karatsuba and traditional schoolbook multiplication approaches. Moreover, the multiplier resources are reused to implement the modular squares and addition chains of the Itoh-Tsujii algorithm for inverse computations. The reuse of resources reduces the overall area requirements. The implementation is performed in Verilog (HDL). The achieved results are provided on Xilinx Virtex 7 device. In addition, the performance of the proposed design is evaluated on ASIC 65 nm process technology. Consequently, a figure-of-merit is constructed to compare the FPGA and ASIC implementations. An exhaustive comparison to existing designs in the literature shows that the proposed architecture utilizes less area. Therefore, the proposed design is the right choice for area-constrained cryptographic applications.
A Comparative Analysis of Two Mediterranean Tornado Hotspots
Authors: Avolio Elenio , Miglietta Mario Marcello
Published: January 2023

An updated climatology of EF1+ (Enhanced Fujita category 1 or stronger) tornadoes in Italy (445 events in the period of 1990–2021) confirms that the central Tyrrhenian (CT) and the southeastern (SE) regions of the Italian peninsula represent two of the areas most affected by tornadoes in the Mediterranean. We performed a comparative analysis between these two hotspots, examining the large-scale atmospheric features associated to the tornado occurrences, throughout the analysis of radiosounding observations and reanalysis (ERA5) fields. The conditions in which the tornadoes develop are investigated using metrics regarding atmospheric instability and/or horizontal/vertical wind shear. Common synoptic characteristics are found for both regions, i.e., a prevalent occurrence of tornadoes on the eastern flank of approaching troughs, with moderate shear/CAPE mean values. A large number of events develop in a high-shear/low-CAPE (HSLC) environment, in the presence of positive sea surface and 850 hPa temperature anomalies. Conversely, the upper-level winds suggest different characteristics of the tornado-spawning cells in the two areas, i.e., multi-cells/linear systems for CT and isolated supercells for SE. The maximum values for some typical atmospheric fields/instability parameters in the areas around the tornado hotspots permit the obtention of thresholds that identify the most favorable environments for tornado formation in these Italian areas.
General Method Based on Regressive Relationships to Parameterize the Three-Parameter Depth–Duration–Frequency Curve
Authors: Mottahedin Amirabbas , Giudicianni Carlo , Barbero Giuseppe , Petaccia Gabriella , Creaco Enrico
Published: January 2023

This paper aims to present simple regressive equations to estimate the parameters of the three-parameter depth–duration–frequency (DDF) curve (3p-DDF), which accurately expresses, for a preassigned return period, the relationship between the rainfall depth and the rainfall duration over large duration ranges, from below 1 h (i.e., tens of minutes) to above 1 h (up to 24 h). These equations are developed to relate their parameters to those of the two-parameter DDF curve (2p-DDF), which can be estimated more easily being based on more readily available data related to rainfall durations above 1 h. In the applications, the regressive equations are first calibrated using recent pluviographic data in northern Italy, Germany, and Sweden. Two validation steps are then carried out to test the equations in terms of estimated rainfall depths using the same data as those used in the calibration step and data of stations from other geographic areas, i.e., Sicily in southern Italy, and from the past century, respectively. The results obtained prove this methodology capable of providing reliable estimation of short-duration rainfalls with various return periods in the absence of measurements with fine temporal resolution.
Integrated Life Cycle Analysis of Cost and CO2 Emissions from Vehicles and Construction Work Activities in Highway Pavement Service Life
Authors: Liu Yuanyuan , Li Haijie , Wang Huihui , Wang Yuanqing , Han Shuang
Published: January 2023

In this study, we aimed to provide a practical method to estimate the economic and environmental impact of vehicle and work activities throughout the entire service life of a pavement area to support pavement management strategies and decisions. To achieve this, we integrated two key life cycle analysis methods, life cycle assessment (LCA)) and life cycle cost analysis (LCCA). The integrated model not only considers CO2 emissions associated with the four main modules—the materials module, the work activities module, the work zone module and the usage module—mentioned in LCA, it also considers agency costs and user costs related to highway usage, work activities, work zone traffic delays and detours in the LCCA process. We used detailed and integrated methods to compute CO2 emissions and costs based on the four modules and two components of agency and users mentioned above. A case study based on a real freeway project in China was used to verify the applicability of the integrated model. The results of the application of the integrated LCA-LCCA model indicate that maintaining the typical activity profile could be beneficial in terms of both CO2 emissions and cost, with savings of 36.8 ton/lane/km of CO2 emissions and 10,530 USD/lane/km (in 2007 dollars) representing the total benefits during the pavement’s service life. This means that timely maintenance could help to achieve savings in terms of financial costs and CO2 emissions simultaneously.
An Improved Battery Equalizer with Reduced Number of Components Applied to Electric Vehicles
Authors: Alvarez-Diazcomas Alfredo , Rodríguez-Reséndiz Juvenal , Carrillo-Serrano Roberto V.
Published: January 2023

The investigation of electric vehicle technologies has increased significantly in the last few years. These vehicles can substantially reduce the environmental impact of the transportation sector. In electric cars, the battery is a crucial element. The batteries are made up of several stacked cells to meet the requirements of the propulsion system. Battery equalizer circuits take active measures to ensure that a particular variable is kept inside an allowable range in all cells. Inductor-based equalizers are very popular since the equalization current is controlled. This paper proposes a single-inductor architecture with a reduced number of components. The proposed topology can transfer energy from adjacent cell-to-cell or adjacent string-to-string. This paper analyzes the operation of the converter, its design, and the design of the controller. Furthermore, a comparison of the proposed equalizer with other inductor-based schemes was made considering the component count, stress on devices, equalization time, driver complexity, and other parameters. The theoretical efficiency of the proposed equalizer obtained was 84.9%, which is competitive with other literature solutions. The impact of battery size on the number of circuit components was also analyzed. Finally, simulation results in open load and changes of current through the battery conditions were performed to validate the theoretical analysis.
Small Intestinal Microbiota Oscillations, Host Effects and Regulation—A Zoom into Three Key Effector Molecules
Authors: Ratiner Karina , Fachler-Sharp Tahel , Elinav Eran
Published: January 2023

The gut microbiota features a unique diurnal rhythmicity which contributes to modulation of host physiology and homeostasis. The composition and activity of the microbiota and its secreted molecules influence the intestinal milieu and neighboring organs, such as the liver. Multiple immune-related molecules have been linked to the diurnal microbiota-host interaction, including Reg3γ, IgA, and MHCII, which are secreted or expressed on the gut surface and directly interact with intestinal bacteria. These molecules are also strongly influenced by dietary patterns, such as high-fat diet and time-restricted feeding, which are already known to modulate microbial rhythms and peripheral clocks. Herein, we use Reg3γ, IgA, and MHCII as test cases to highlight the divergent effects mediated by the diurnal activity of the gut microbiota and their downstream host effects. We further highlight current challenges and conflicts, remaining questions, and perspectives toward a holistic understanding of the microbiome’s impacts on circadian human behavior.
The Co-Association of Enterobacteriaceae and Pseudomonas with Specific Resistant Cucumber against Fusarium Wilt Disease
Authors: Zhang Yu-Lu , Guo Xiao-Jing , Huang Xin , Guo Rong-Jun , Lu Xiao-Hong , Li Shi-Dong , Zhang Hao
Published: January 2023

The root microbiota contributes to the plant’s defense against stresses and pathogens. However, the co-association pattern of functional bacteria that improves plant resistance has not been interpreted clearly. Using Illumina high-throughput sequencing technology, the root bacterial community profiles of six cucumber cultivars with different resistance in response to the causative agent of cucumber Fusarium wilt (CFW), Fusarium oxysporum f. sp. cucumerinum (Foc), were analyzed. The principal coordinate analysis indicated that the interactions of the cultivars and pathogens drove the cucumber root bacterial communities (p = 0.001). The resistance-specific differential genera across the cultivars were identified, including Massilia in the resistant cultivars, unclassified Enterobacteriaceae in resistant CL11 and JY409, Pseudomonas in JY409, Cronobacter in moderately resistant ZN106, and unclassified Rhizobiaceae and Streptomyces in susceptible ZN6. The predominant root bacterium Massilia accounted for the relative abundance of up to 28.08–61.55%, but dramatically declined to 9.36% in Foc-inoculated susceptible ZN6. Pseudomonas ASV103 and ASV48 of Pseudomonadaceae and Cronobacter ASV162 of Enterobacteriaceae were consistently differential across the cultivars at the phylum, genus, and ASV levels. Using the culture-based method, antagonistic strains of Enterobacteriaceae with a high proportion of 51% were isolated. Furthermore, the bacterial complexes of Pantoea dispersa E318 + Pseudomonas koreensis Ps213 and Cronobacter spp. C1 + C7 reduced the disease index of CFW by 77.2% and 60.0% in the pot experiment, respectively. This study reveals the co-association of specific root bacteria with host plants and reveals insight into the suppressing mechanism of resistant cultivars against CFW disease by regulating the root microbiota.
Identification of an l-Arabitol Transporter from Aspergillus niger
Authors: Meng Jiali , Mäkelä Miia R. , de Vries Ronald P.
Published: January 2023

l-arabitol is an intermediate of the pentose catabolic pathway in fungi but can also be used as a carbon source by many fungi, suggesting the presence of transporters for this polyol. In this study, an l-arabitol transporter, LatA, was identified in Aspergillus niger. Growth and expression profiles as well as sugar consumption analysis indicated that LatA only imports l-arabitol and is regulated by the arabinanolytic transcriptional activator AraR. Moreover, l-arabitol production from wheat bran was increased in a metabolically engineered A. niger mutant by the deletion of latA, indicating its potential for improving l-arabitol-producing cell factories. Phylogenetic analysis showed that homologs of LatA are widely conserved in fungi.
Age at Onset Influences Progression of Motor and Non-Motor Symptoms during the Early Stage of Parkinson’s Disease: A Monocentric Retrospective Study
Authors: De Carolis Lanfranco , Galli Silvia , Bianchini Edoardo , Rinaldi Domiziana , Raju Manikandan , Caliò Bianca , Alborghetti Marika , Pontieri Francesco E.
Published: January 2023

The interactions between the age at onset with other pathogenic mechanisms and the interplays between the disease progression and the aging processes in Parkinson’s disease (PD) remain undefined, particularly during the first years of illness. Here, we retrospectively investigated the clinical presentation and evolution of the motor and non-motor symptoms and treatment-related complications during the first 5 years of illness in subjects categorized according to age at onset. A total of 131 subjects were divided into “Early-Onset-PD” (EOPD; onset ≤49 years), “Middle-Onset-PD” (MOPD; onset 50–69 years) and “Late-Onset-PD” (LOPD; onset ≥70 years). The T0 visit was set at the time of the clinical diagnosis; the T1 visit was 5 years (±5 months) later. At T0, there were no significant differences in the motor features among the groups. At T1, the LOPD patients displayed a significantly higher frequency of gait disturbances and a higher frequency of postural instability. Moreover, at T1, the LOPD subjects reported a significantly higher frequency of non-motor symptoms; in particular, cardiovascular, cognitive and neuropsychiatric domains. The presented results showed a significantly different progression of motor and non-motor symptoms in the early course of PD according to the age at onset. These findings contribute to the definition of the role of age at onset on disease progression and may be useful for the pharmacological and non-pharmacological management of PD.
Evaluating Smart Home Services and Items: A Living Lab User Experience Study
Authors: Seo Eugene , Yang Wanseok
Published: January 2023

South Korea is expected to become a super-aged society by 2025, when more than 20% of its population will be aged 65 and over. One possible solution for minimizing the socioeconomic burden posed by this aging trend is smart home technology, which can be used to support older adults’ daily routines. In this study, the aim is to suggest the direction of development on smart home technology and products to enhance our understanding of service and item needs for the optimal commercialization of smart homes for users. A living lab was set up to research the experience of using smart home technology in real environments. To obtain intuitive and specific evaluations, visitors of varying ages tested each item and living space and responded to a five-point scale questionnaire on importance (recognition) and performance (satisfaction). The recognition and satisfaction for each smart home item or service were then evaluated using basic statistical analysis, importance–performance analysis, and factor analysis using SPSS. It was determined that the importance and performance evaluations of smart home services and items differed by age group. The scores for the two categories exhibited evident similarities in the older adult group. More similarities were found in the evaluation of performance than importance across age groups. The results show that different age groups agree that the development of services/items that can constantly and automatically check residents’ health status should be prioritized.
Thermal Assessment of a Ventilated Double Skin Façade Component with a Set of Air Filtering Photocatalytic Slats in the Cavity
Authors: Roig Oriol , Cuerva Eva , Pardal Cristina , Guardo Alfredo , Isalgue Antonio , Lopez-Besora Judit
Published: January 2023

Indoor air quality is a crucial factor when evaluating habitability, especially in developed countries, where people spend most of their time indoors. This paper presents a novel double skin façade (DSF) system that combines physical and photocatalytic filtering strategies. The air purification system is made up of fixed slats that are both solar protection and air purification system. The objective of this work is to determine the thermal behaviour of the proposed system, so that its suitability for use in various environments may be assessed. This was carried out using a physical 1:1 scale model and a computational fluid dynamics (CFD) model. The maximum temperature inside the scale model cavity was 17–20 °C higher than outdoor air. Additionally, it was discovered that the airflow through the DSF would require forced ventilation. To determine the emissivity values of the photocatalytic coating, additional experimental measurements were made. The CFD model was tested for summer and winter conditions in Barcelona, Chicago, and Vancouver. The average increase in the intake air temperature was around 14.5 °C in winter and 12 °C in summer, finding that the system has its main use potential in temperate or cold areas with many hours of solar radiation.
Recurrence Risk Evaluation in Patients with Papillary Thyroid Carcinoma: Multicenter Machine Learning Evaluation of Lymph Node Variables
Authors: Jang Sung-Woo , Park Jae-Hyun , Kim Hae-Rim , Kwon Hyeong-Ju , Lee Yu-Mi , Hong Suck-Joon , Yoon Jong-Ho
Published: January 2023

Background: Lymph node (LN)-related risk factors have been updated to predict long-term outcomes in patients with papillary thyroid carcinoma (PTC). However, those factors’ analytic appropriateness and general applicability must be validated. This study aimed to assess LN-related risk factors, and suggest new LN-related risk categories. Methods: This multicenter observational cohort study included 1232 patients with PTC with N1 disease treated with a total thyroidectomy and neck dissection followed by radioactive iodine remnant ablation. Results: The median follow-up duration was 117 months. In the follow-up period, structural recurrence occurred in 225 patients (18.3%). Among LN-related variables, the presence of extranodal extension (p < 0.001), the maximal diameter of metastatic LN foci (p = 0.029), the number of retrieved LNs (p = 0.003), the number of metastatic LNs (p = 0.003), and the metastatic LN ratio (p < 0.001) were independent risk factors for structural recurrence. Since these factors showed a nonlinear association with the hazard ratio of recurrence-free survival (RFS) rates, we calculated their optimal cutoff values using the K-means clustering algorithm, selecting 0.2 cm and 1.1 cm for the maximal diameter of metastatic LN foci, 4 and 13 for the number of metastatic LN, and 0.28 and 0.58 for the metastatic LN ratio. The RFS curves of each subgroup classified by these newly determined cutoff values showed significant differences (p < 0.001). Each LN risk group also showed significantly different RFS rates from the others (p < 0.001). Conclusions: In PTC patients with an N1 classification, our novel LN-related risk estimates may help predict long-term outcomes and design postoperative management and follow-up strategies. After further validation studies based on independent datasets, these risk categories might be considered when redefining risk stratification or staging systems.
Graph Theory Measures and Their Application to Neurosurgical Eloquence
Authors: Tanglay Onur , Dadario Nicholas B. , Chong Elizabeth H. N. , Tang Si Jie , Young Isabella M. , Sughrue Michael E.
Published: January 2023

Improving patient safety and preserving eloquent brain are crucial in neurosurgery. Since there is significant clinical variability in post-operative lesions suffered by patients who undergo surgery in the same areas deemed compensable, there is an unknown degree of inter-individual variability in brain ‘eloquence’. Advances in connectomic mapping efforts through diffusion tractography allow for utilization of non-invasive imaging and statistical modeling to graphically represent the brain. Extending the definition of brain eloquence to graph theory measures of hubness and centrality may help to improve our understanding of individual variability in brain eloquence and lesion responses. While functional deficits cannot be immediately determined intra-operatively, there has been potential shown by emerging technologies in mapping of hub nodes as an add-on to existing surgical navigation modalities to improve individual surgical outcomes. This review aims to outline and review current research surrounding novel graph theoretical concepts of hubness, centrality, and eloquence and specifically its relevance to brain mapping for pre-operative planning and intra-operative navigation in neurosurgery.
Patients with Chronic Liver Disease under Surveillance for Hepatocellular Carcinoma Have a Favorable Long-Term Outcome for Pancreatic Cancer Due to Early Diagnosis and High Resection Rate
Authors: Kumagi Teru , Terao Takashi , Kuroda Taira , Koizumi Mitsuhito , Imamura Yoshiki , Ohno Yoshinori , Yokota Tomoyuki , Azemoto Nobuaki , Uesugi Kazuhiro , Kisaka Yoshiyasu , Tanaka Yoshinori , Shibata Naozumi , Miyata Hideki , Miyake Teruki , Hiasa Yoichi
Published: January 2023

Patients with viral hepatitis-related chronic liver disease (CLD) under surveillance for hepatocellular carcinoma (HCC) are often diagnosed with pancreatic cancer (PC) at an early stage. However, the long-term outcomes of these patients are unclear. We aimed to clarify the long-term outcomes of patients with PC with viral hepatitis-related CLD using a chart review. Data collection included the Union for International Cancer Control (UICC) stage at PC diagnosis, hepatitis B virus and hepatitis C virus status, and long-term outcomes. The distribution of the entire cohort (N = 552) was as follows: early stage (UICC 0-IB; n = 52, 9.5%) and non-early stages (UICC IIA-IV; n = 500, 90.5%). At diagnosis, the HCC surveillance group (n = 18) had more patients in the early stages than the non-surveillance group (n = 534) (50% vs. 8.0%), leading to a higher indication rate for surgical resection (72.2% vs. 29.8%) and a longer median survival time (19.0 months vs. 9.9 months). We confirmed that patients with viral hepatitis-related CLD under HCC surveillance were diagnosed with PC at an early stage. Because of the higher indication rate for surgical resection in these patients, they had favorable long-term outcomes for PC.
Discovery and Heterologous Expression of Unspecific Peroxygenases
Authors: Ebner Katharina , Pfeifenberger Lukas J. , Rinnofner Claudia , Schusterbauer Veronika , Glieder Anton , Winkler Margit
Published: January 2023

Since 2004, unspecific peroxygenases, in short UPOs (EC. 1.11.2.1), have been explored. UPOs are closing a gap between P450 monooxygenases and chloroperoxidases. These enzymes are highly active biocatalysts for the selective oxyfunctionalisation of C–H, C=C and C-C bonds. UPOs are secreted fungal proteins and Komagataella phaffii (Pichia pastoris) is an ideal host for high throughput screening approaches and UPO production. Heterologous overexpression of 26 new UPOs by K. phaffii was performed in deep well plate cultivation and shake flask cultivation up to 50 mL volume. Enzymes were screened using colorimetric assays with 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,6-dimethoxyphenol (DMP), naphthalene and 5-nitro-1,3-benzodioxole (NBD) as reporter substrates. The PaDa-I (AaeUPO mutant) and HspUPO were used as benchmarks to find interesting new enzymes with complementary activity profiles as well as good producing strains. Herein we show that six UPOs from Psathyrella aberdarensis, Coprinopsis marcescibilis, Aspergillus novoparasiticus, Dendrothele bispora and Aspergillus brasiliensis are particularly active.