bluuebunny/crossref_metadata_2025 huggingface dataset:
Noncaloric monosaccharides induce excessive sprouting angiogenesis in zebrafish via foxo1a-marcksl1a signal
Authors: Wang Xiaoning , Zhao Jinxiang , Xu Jiehuan , Li Bowen , Liu Xia , Xie Gangcai , Duan Xuchu , Liu Dong
Published: April 2024

Artificially sweetened beverages containing noncaloric monosaccharides were suggested as healthier alternatives to sugar-sweetened beverages. Nevertheless, the potential detrimental effects of these noncaloric monosaccharides on blood vessel function remain inadequately understood. We have established a zebrafish model that exhibits significant excessive angiogenesis induced by high glucose, resembling the hyperangiogenic characteristics observed in proliferative diabetic retinopathy (PDR). Utilizing this model, we observed that glucose and noncaloric monosaccharides could induce excessive formation of blood vessels, especially intersegmental vessels (ISVs). The excessively branched vessels were observed to be formed by ectopic activation of quiescent endothelial cells (ECs) into tip cells. Single-cell transcriptomic sequencing analysis of the ECs in the embryos exposed to high glucose revealed an augmented ratio of capillary ECs, proliferating ECs, and a series of upregulated proangiogenic genes. Further analysis and experiments validated that reduced foxo1a mediated the excessive angiogenesis induced by monosaccharides via upregulating the expression of marcksl1a. This study has provided new evidence showing the negative effects of noncaloric monosaccharides on the vascular system and the underlying mechanisms.
Noncaloric monosaccharides induce excessive sprouting angiogenesis in zebrafish via foxo1a-marcksl1a signal
Authors: wang Xiaoning , Zhao Jinxiang , Xu Jiehuan , Li Bowen , Liu Xia , Xie Gangcai , Duan Xuchu , Liu Dong
Published: April 2024

Artificially sweetened beverages containing noncaloric monosaccharides were suggested as healthier alternatives to sugar-sweetened beverages. Nevertheless, the potential detrimental effects of these noncaloric monosaccharides on blood vessel function remain inadequately understood. Presently, we have established a zebrafish model that exhibits significant excessive angiogenesis induced by high glucose, resembling the hyperangiogenic characteristics observed in proliferative diabetic retinopathy (PDR). Utilizing this model, we observed that glucose and noncaloric monosaccharides could induce excessive formation of blood vessels, especially intersegmental vessels (ISVs). The excessively branched vessels were observed to be formed by ectopic activation of quiescent endothelial cells (ECs) into tip cells. Single-cell transcriptomic sequencing analysis of the endothelial cells in the embryos exposed to high glucose revealed an augmented ratio of capillary ECs, proliferating ECs, and a series of upregulated proangiogenic genes. Further analysis and experiments validated that foxo1a mediated the excessive angiogenesis induced by monosaccharides by down-regulating the expression of
marcksl1a
. This study has provided new evidence showing the negative effects of noncaloric monosaccharides on the vascular system and the underlying mechanisms.
Allosteric coupling asymmetry mediates paradoxical activation of BRAF by type II inhibitors
Authors: Rasmussen Damien M , Semonis Manny M , Greene Joseph T , Muretta Joseph M , Thompson Andrew R , Toledo Ramos Silvia , Thomas David D , Pomerantz William CK , Freedman Tanya S , Levinson Nicholas M
Published: April 2024

The type II class of RAF inhibitors currently in clinical trials paradoxically activate BRAF at subsaturating concentrations. Activation is mediated by induction of BRAF dimers, but why activation rather than inhibition occurs remains unclear. Using biophysical methods tracking BRAF dimerization and conformation, we built an allosteric model of inhibitor-induced dimerization that resolves the allosteric contributions of inhibitor binding to the two active sites of the dimer, revealing key differences between type I and type II RAF inhibitors. For type II inhibitors the allosteric coupling between inhibitor binding and BRAF dimerization is distributed asymmetrically across the two dimer binding sites, with binding to the first site dominating the allostery. This asymmetry results in efficient and selective induction of dimers with one inhibited and one catalytically active subunit. Our allosteric models quantitatively account for paradoxical activation data measured for 11 RAF inhibitors. Unlike type II inhibitors, type I inhibitors lack allosteric asymmetry and do not activate BRAF homodimers. Finally, NMR data reveal that BRAF homodimers are dynamically asymmetric with only one of the subunits locked in the active αC-in state. This provides a structural mechanism for how binding of only a single αC-in inhibitor molecule can induce potent BRAF dimerization and activation.
Discarded sequencing reads uncover natural variation in pest resistance in Thlaspi arvense
Authors: Galanti Dario , Jung Jun Hee , Müller Caroline , Bossdorf Oliver
Published: April 2024

Understanding the genomic basis of natural variation in plant pest resistance is an important goal in plant science, but it usually requires large and labor-intensive phenotyping experiments. Here, we explored the possibility that non-target reads from plant DNA sequencing can serve as phenotyping proxies for addressing such questions. We used data from a whole-genome and -epigenome sequencing study of 207 natural lines of field pennycress (Thlaspi arvense) that were grown in a common environment and spontaneously colonized by aphids, mildew, and other microbes. We found that the numbers of non-target reads assigned to the pest species differed between populations, had significant SNP-based heritability, and were associated with climate of origin and baseline glucosinolate contents. Specifically, pennycress lines from cold and thermally fluctuating habitats, presumably less favorable to aphids, showed higher aphid DNA load, i.e., decreased aphid resistance. Genome-wide association analyses identified genetic variants at known defense genes but also novel genomic regions associated with variation in aphid and mildew DNA load. Moreover, we found several differentially methylated regions associated with pathogen loads, in particular differential methylation at transposons and hypomethylation in the promoter of a gene involved in stomatal closure, likely induced by pathogens. Our study provides first insights into the defense mechanisms of Thlaspi arvense, a rising crop and model species, and demonstrates that non-target whole-genome sequencing reads, usually discarded, can be leveraged to estimate intensities of plant biotic interactions. With rapidly increasing numbers of large sequencing datasets worldwide, this approach should have broad application in fundamental and applied research.
Endocytic recycling is central to circadian collagen fibrillogenesis and disrupted in fibrosis
Authors: Chang Joan , Pickard Adam , Herrera Jeremy A. , O’Keefe Sarah , Hartshorn Matthew , Garva Richa , Hoyle Anna , Dingle Lewis , Zeltz Cédric , Wong Jason , Reid Adam , Venkateswaran Rajamiyer V. , Lu Yinhui , Caswell Patrick , High Stephen , Gullberg Donald , Kadler Karl E.
Published: April 2024

Collagen-I fibrillogenesis is crucial to health and development, where dysregulation is a hallmark of fibroproliferative diseases. Here, we show that collagen-I fibril assembly required a functional endocytic system that recycles collagen-I to assemble new fibrils. Endogenous collagen production was not required for fibrillogenesis if exogenous collagen was available, but the circadian-regulated vacuolar protein sorting (VPS) 33b and collagen-binding integrin-α11 subunit were crucial to fibrillogenesis. Cells lacking VPS33b secrete soluble collagen-I protomers but were deficient in fibril formation, thus secretion and assembly are separately controlled. Overexpression of VPS33b led to loss of fibril rhythmicity and over-abundance of fibrils, which was mediated through integrin α11β1. Endocytic recycling of collagen-I was enhanced in human fibroblasts isolated from idiopathic pulmonary fibrosis, where VPS33b and integrin-α11 subunit were overexpressed at the fibrogenic front; this correlation between VPS33b, integrin-α11 subunit, and abnormal collagen deposition was also observed in samples from patients with chronic skin wounds. In conclusion, our study showed that circadian-regulated endocytic recycling is central to homeostatic assembly of collagen fibrils and is disrupted in diseases.
Artificially inserted strong promoter containing multiple G-quadruplexes induces long-range chromatin modification
Authors: Roy Shuvra Shekhar , Bagri Sulochana , Vinayagamurthy Soujanya , Sengupta Avik , Then Claudia Regina , Kumar Rahul , Sridharan Sriram , Chowdhury Shantanu
Published: April 2024

Although the role of G-quadruplex (G4) DNA structures has been suggested in chromosomal looping this was not tested directly. Here, to test causal function, an array of G4s, or control sequence that does not form G4s, were inserted within chromatin in cells. In vivo G4 formation of the inserted G4 sequence array, and not the control sequence, was confirmed using G4-selective antibody. Compared to the control insert, we observed a remarkable increase in the number of 3D chromatin looping interactions from the inserted G4 array. This was evident within the immediate topologically associated domain (TAD) and throughout the genome. Locally, recruitment of enhancer histone marks and the transcriptional coactivator p300/Acetylated-p300 increased in the G4-array, but not in the control insertion. Resulting promoter-enhancer interactions and gene activation were clear up to 5 Mb away from the insertion site. Together, these show the causal role of G4s in enhancer function and long-range chromatin interactions. Mechanisms of 3D topology are primarily based on DNA-bound architectural proteins that induce/stabilize long-range interactions. Involvement of the underlying intrinsic DNA sequence/structure in 3D looping shown here therefore throws new light on how long-range chromosomal interactions might be induced or maintained.
Preparation and characterization of vitamin E/calcium/soy protein isolate nanoparticles for soybean milk beverage fortification
Authors: Taha Heba A. I. M. , Agamy Neveen F. M. , Soliman Tarek N. , Younes Nashwa M. , El-Enshasy Hesham Ali , Darwish Amira M. G.
Published: April 2024

Soybean milk is a rich plant-based source of protein, and phenolic compounds. This study compared the nutritional value of soybean milk, flour, soy protein isolate (SPI) and evaluated the impact of prepared vitamin E/calcium salt/soy protein isolate nanoparticles (ECSPI-NPs) on fortification of developed soybean milk formulations. Results indicated that soybean flour protein content was 40.50 g/100 g, that fulfills 81% of the daily requirement (DV%), the unsaturated fatty acids (USFs), oleic and linoleic content was 21.98 and 56.7%, respectively, of total fatty acids content. In soybean milk, essential amino acids, threonine, leucine, lysine achieved 92.70, 90.81, 77.42% of amino acid scores (AAS) requirement values respectively. Ferulic acid was the main phenolic compound in soybean flour, milk and SPI (508.74, 13.28, 491.78 µg/g). Due to the moisture content of soybean milk (88.50%) against (7.10%) in soybean flour, the latest showed higher nutrients concentrations. The prepared calcium (20 mM/10 g SPI) and vitamin E (100 mg/g SPI) nanoparticles (ECSPI-NPs) exhibited that they were effectively synthesized under transmission electron microscope (TEM), stability in the zeta sizer analysis and safety up to IC50value (202 ug/mL) on vero cell line. ECSPI-NPs fortification (NECM) enhanced significantly phenolic content (149.49 mg/mL), taste (6.10), texture (6.70) and consumer overall acceptance (6.54). Obtained results encourage the application of the prepared ECSPI-NPs for further functional foods applications.
Calidad y contenido de calcio en frutos de cerezo dulce provenientes de ramas de árboles conducidos en KGB y sometidas a restricciones de haces vasculares
Authors:   , Quiroz González María Paz
Published: April 2024

La producción de cereza dulce (Prunus avium L.) se ha incrementado en los últimos años en Chile, siendo su principal destino Asia. Para llegar a este destino, con fruta de calidad es necesario tener una adecuada relación fuente-sumidero, además de extender la vida útil poscosecha, donde el nutriente calcio (Ca) juega un rol fundamental. Los ensayos se realizaron en un huerto comercial de cultivar “Lapins” / “Colt”, conducido en KGB. En el Exp 1 se comparó la concentración de Ca y la calidad a cosecha de la fruta proveniente de ramas de cerezo tratadas con restricciones floemáticas y xilemáticas. Además, se monitoreó el desarrollo vegetativo y de frutos durante la temporada y el estatus hídrico de las ramas intervenidas. Se consideraron cinco tratamientos (TR) en 10 bloques. Los TR fueron: TR1 - C; sin restricciones, TR2 - A; anillado en la base, TR3 - DA; anillado en la base y otro en la unión entre el segundo y tercer año de crecimiento, TR4 - S; corte al 50% del xilema y TR5 - SA; anillado en la base más corte al 50% del xilema. El TR1 - C tuvo el mayor AF por brote, número de frutos por rama y la distribución más homogénea de color y calibre. También, mantuvo Ψtallo y gs mayores que los TR con alguna intervención. El TR3 - DA registró mayor número de brotes y relación H·F-1. La mitad superior de TR3 - DA alcanzó el mayor diámetro, PF, PS, S.S., AT y la menor firmeza entre los TR. Además, la menor concentración de Ca, Mg y K en los frutos. El anillado en el resto de los TR no mejoró la calidad ni la concentración de nutrientes en la fruta en el resto de los TR. En el Exp 2 se cuantificó la superficie xilemática que presentaba tinción luego de sumergir ramas en fucsina ácida. Los TR fueron TR1-S y TR2-SA, en ambos aumentó la intensidad de color al alejarse de la incisión.
Caracterización de cabernet-sauvignon de Chile: investigación de factores vitícolas y bioclimáticos en la composición química de la uva
Authors:   , García Martínez Jorge de Jesús
Published: April 2024

La composición de la uva y la calidad del vino son determinadas en gran parte por factores vitícolas que varían de un viñedo a otro. Utilizando una muestra de 39 parcelas de Cabernet Sauvignon comerciales repartidas en el valle central de Chile en una diversidad de climas y suelos y con diferentes sistemas de conducción, edades, clones, injertados o no, y con distintas prácticas vitícolas se estudió el efecto de algunos de estos factores sobre la composición general y algunos marcadores fenólicos de la uva. Los sistemas de conducción producto de las modificaciones de la canopia y efectos del vigor y rendimiento influyen sobre parámetros básicos y flavonoles. Las plantas masales se correlaciona con riqueza y madurez fenólica; el mismo efecto se asocia a plantaciones en pie franco versus injertos. El análisis de clima con orientación norte a sur categoriza al Valle del Maipo en la zona II, área con mejores condiciones para el desarrollo del Cabernet Sauvignon; en el análisis este a oeste muestra resultados claros e importantes entre los marcadores químicos habiendo mayor contenido de flavonoles y flavan-3-oles para la zona Andes.
Flores húmedas, diario húmedo
Authors:   , Mendoza Rajevic Vicenta
Published: April 2024

La presente investigación propone una reflexión en torno a mi trabajo desarrollado durante el programa de magíster y que aborda, desde la fotografía y la instalación, los imaginarios femeninos que giran en torno a la sexualidad y el erotismo, desde una perspectiva feminista. Esta memoria está estructurada a partir de un marco teórico que es leído bajo el sesgo crítico del giro afectivo que pone el énfasis en los imaginarios y las representaciones de las subjetividades femeninas. En el capítulo introductorio se exponen los principales marcos teóricos vinculados a los imaginarios de mi trabajo.Posteriormente, y a partir de este cuadro analítico, se observan en el capítulo siguiente algunos referentes visuales, tanto editoriales como fotográficos, con los que mi trabajo dialoga.Por último, se lleva a cabo la memoria y el análisis del proyecto resultante, incorporando descripciones, procesos de trabajo, bitácoras y reflexiones.
Evaluación formativa y compartida en una investigación en ciencias
Authors:   , Krippel Ponde de León Paulina
Published: April 2024

La evaluación es un proceso fundamental para el aprendizaje y con la implementación en Chile del Decreto 67/2018. La diversificación de la evaluación y la evaluación formativa cobran real importancia, lo que genera un desafío a los docentes que por muchos años hay evaluado a sus estudiantes en su mayoría, con instrumentos tipo pruebas. Por otra parte, los alumnos del sistema educativo chileno no tienen demasiada participación en este proceso evaluativo, la cual debería ser mucho mayor. Para determinar el impacto de la implementación de un proceso de evaluación formativa y compartida, este trabajo realizó un análisis de los cambios en las reflexiones de los estudiantes, sustentando esta investigación en un diseño de investigación-acción desde un paradigma crítico, donde se busca justicia y cambio social. El análisis de los datos se realizó de manera cualitativa, haciendo una triangulación de la información recogida por medio de bitácoras, grabaciones y rúbricas. La validación de los instrumentos se realizó de manera dialógica con estudiantes y participantes del establecimiento donde se desarrolla la intervención. Finalmente, como resultado y conclusión de esta investigación, se observaron cambios en las reflexiones de los estudiantes al poder tomar decisiones en su proceso evaluativo de manera protagónica y crítica, desarrollando autorregulación por medio de auto y coevaluaciones.
Proyecto de mejoramiento: Modelo de gestión de dispositivos de capacitación on-line de competencias pedagógicas en una institución de Educación Superior
Authors:   , Retamales Chacana José Francisco
Published: April 2024

Una de las preocupaciones y punto de discusión actual en el ámbito de la educación superior en Chile es la dimensión de la docencia; esto es, con qué herramientas pedagógicas cuenta el docente para desempeñarse en las aulas de la educación superior (Mas-Torelló & Olmos-Rueda, 2016). Este proyecto, por lo anterior, presenta en una sede local de una propuesta de intervención y mejoramiento de un modelo estandarizado de gestión de formación docente de una institución de formación profesional con cobertura nacional. La intervención de este proyecto consideró entonces la incorporación de docentes monitores con tareas específicas de apoyo en la implementación en sede de una parte de este plan de formación, así como el desarrollo de iniciativas desde la gestión de los recursos humanos y económicos del colaborador responsable en sede de la ejecución de dicho plan. Los resultados mostraron un importante avance en efectividad y eficiencia en el uso de los recursos involucrados, bajando el costo empresa y aumentando el nivel de aprobación de los docentes participantes.
La relación de las oportunidades de aprendizaje del conocimiento docente en las carreras de pedagogía básica sobre la autopercepción en tareas docentes de profesores en formación
Authors:   , Vega Godoy Andrea
Published: April 2024

Garantizar la calidad de los estudiantes en formación, así como de los futuros docentes, ha generado la necesidad de evaluar los programas de formación en búsqueda de la calidad, lo que es una tarea bastante compleja por la característica multidimensional, propias de la labor docente. Para contribuir a la discusión, esta investigación describe en profundidad las oportunidades de aprendizaje que ofrecen los programas de formación inicial. El objetivo principal de esta tesis es conocer la relación entre las oportunidades de aprendizaje ofrecidas en los programas de formación de estudiantes de último año de la carrera de Pedagogía de Educación Básica y el conocimiento práctico. Para ello, se realizó un estudio descriptivo correlacional con tres fases: 1° fase: análisis de las carreras pedagógicas en términos de tipo de conocimiento; 2° fase: elaboración y aplicación de un instrumento para recoger la percepción de autoeficacia de los estudiantes respecto a tareas docentes, y 3° fase: relación entre la percepción de autoeficacia con los tipos de conocimientos.
Distributed Local Energy Market for Microgrids based on Feedback Optimization
Authors:   , Díaz Ulloa Alejandro Eduardo
Published: April 2024

A medida que aumenta la penetración de los recursos energéticos distribuidos (DER), se hacen necesarias estrategias de control eficientes y confiables para su operación coordinada dentro de las microrredes. Esta tesis propone una arquitectura de mercado de energía local para microrredes basada en feedback optimization, que lleva a una microrred a un estado estable al resolver un problema subyacente de operación óptima, con las características de ser distribuida y plug-and play. La arquitectura utiliza una red de comunicaciones, un algoritmo de consenso lineal y agentes inteligentes de servicio adicionales que respaldan la toma de decisiones distribuida. Características adicionales incluyen el diseño de una función de costo de energía futura (FCF) para facilitar la gestión del almacenamiento distribuido a lo largo del tiempo y el diseño de un mercado de energía forward para gestionar el riesgo. La arquitectura propuesta es simulada en el despacho óptimo multiperíodo de una microrred aislada de 5 barras, utilizando dos casos de estudio para evaluar el desempeño del controlador. El primer caso de estudio muestra la dinámica de los FCF propuestos para una gestión satisfactoria del almacenamiento en las unidades de almacenamiento distribuido. El segundo caso de estudio analiza la dinámica del mercado de energía forward y su impacto en el controlador principal, implementándose con éxito la subasta de energía. En todos los casos de estudio, el controlador logra alcanzar rápidamente un estado estable óptimo y un consenso de las variables globales entre todos los agentes de la microrred.
Cuantificación de la percepción visual de entornos urbanos a través de imágenes satelitales, utilizando aprendizaje contrastivo multimodal
Authors:   , Magna Depoortere Michel Alexander
Published: April 2024

El estudio de la percepción del entorno urbano permite desarrollar modelos computacionales que identifiquen a gran escala los elementos más significativos que influyen en la percepción de las personas sobre el lugar en el que se desenvuelven. Estos resultados son cruciales para el diseño de áreas de tránsito peatonal o vehicular y la asignación eficiente de recursos con el fin de mejorar las ciudades y sus espacios públicos. Diversos estudios han concluido que un diseño apropiado de los espacios públicos promueve, por ejemplo, un mayor uso del transporte público, áreas verdes, actividades deportivas y contribuye a la salud mental de los residentes. Por lo tanto, resulta relevante desarrollar metodologías que apoyen estas decisiones. Una alternativa para superar las limitaciones mencionadas es el empleo de imágenes satelitales. Estas ofrecen datos más actualizados, recientes y económicos en comparación con Google Street View, gracias a la proliferación de satélites y servicios relacionados. No obstante, existe una limitación significativa: la percepción visual humana se experimenta y reporta a nivel terrestre y desde una perspectiva única, lo que dificulta la aplicación directa de imágenes captadas desde ángulos radicalmente diferentes, como es el caso de las imágenes satelitales. En los últimos años, los modelos computacionales han dependido en gran medida de las imágenes de Google Street View para sus análisis. Esta dependencia ofrece la ventaja de un acceso fácil y masivo a los datos. Sin embargo, en ciertas áreas, la actualización de estas imágenes es un proceso lento, principalmente debido al elevado costo que implica un mapeo exhaustivo de la ciudad, tal como se observa en Chile. Esta situación conduce a un sesgo inherente en las imágenes, presente desde el momento de su captura y durante su uso en los análisis. Este estudio introduce una metodología diseñada para evaluar la percepción del entorno urbano en Santiago, minimizando el sesgo temporal. Se propone un enfoque innovador que integra tanto imágenes de Street View como imágenes satelitales para desarrollar un modelo multimodal de aprendizaje contrastivo. Este modelo es capaz de predecir la percepción basándose en una imagen dada. Dicha metodología aborda el problema de la discrepancia temporal entre la captura de las imágenes y el momento del análisis, resultando además en un proceso más eficiente al reducir la necesidad de actualizar constantemente las imágenes de Google Street View, gracias a la utilización de imágenes satelitales que se actualizan en menor tiempo.
¡Cuida tu Salud Mental! 2023: Intervención psicoeducativa sobre los beneficios del movimiento corporal como hábito saludable para la salud mental
Authors:   , Bravo Hernández Tamara Ailén
Published: April 2024

Las campañas masivas de psicoeducación han sido claves en inducir cambios cognitivos y conductuales beneficiosos, incrementando la alfabetización en salud mental. Actualmente, aunque hay abundante información sobre salud mental en plataformas digitales, muchas no son fuentes fiables ni se enfocan en informar con veracidad. Este escenario presenta desafíos en el acceso a información adecuada y confiable. En este contexto, la tercera edición de la campaña psicoeducativa "¡Cuida tu salud mental!" se propone generar y difundir material psicoeducativo de confianza, proporcionando información basada en evidencia para fomentar la salud mental en la población. Dentro de esta campaña, este trabajo de grado busca promover el movimiento corporal como un hábito saludable para la salud mental, resaltando sus múltiples beneficios. Para realizar la iniciativa, se desarrolló una propuesta psicoeducativa, la cual fue evaluada por 18 expertos y 63 usuarios, usando una metodología de evaluación mixta. Los resultados reflejaron una percepción positiva del material, acompañada de impresiones y recomendaciones para integrar. La difusión del material psicoeducativo audiovisual fue presentada en Metro de Santiago, así como en las plataformas web y redes sociales de instituciones colaboradoras, incluyendo la Pontificia Universidad Católica, la Fundación PsiConecta y el Instituto Milenio para la Investigación en Depresión y Personalidad.
Immunoglobulin Based Therapeutic Approach for Parvo Virus Infection in Dog: A Case Report
Authors: Deka Naba Jyoti , Kalita Dhruba Jyoti , Chabukdhara Prasanta , Brahma Derhasar , Dutta Chinmoy , Bania Biraj
Published: April 2024

Canine parvo viral infection is a highly contagious disease in dog with high mortality rate in untreated cases. This study represents a case report of an unvaccinated 4 months old German shepherd puppy presented with a history of profuse foetid diarrhoea, vomition, inappetence and depression for 2 days. Physical examination of the dog revealed sub normal temperature (99.5°F), abdominal pain, bloat, severe dehydration and debility. Haematology showed low RBC count, low Hb level, low Hct value, lower MCV and mild lymphopenia. The dog was diagnosed as canine parvoviral infection based on the result of rapid antigen test and the characteristic clinical findings. The treatment included fluid replacement therapy, antimicrobial drug, antiemetic, haemostatic agent along with immunoglobulins. An instant onset of passive immunity is developed upon IV administration of immunoglobulins. The pup showed good response from 3rd day of the treatment and finally recovered successfully. Thus, administration of specific immunoglobins along with standard treatment regimen in canine parvo virus infection can be effective in reducing mortality in dogs.
Apple Cider Vinegar Effervescent Tablets on Gut Health, Obesity and User Experience: An Observation
Authors: Kannan S. , Anandhasayanam A. , E.S Niranjana , Rajamurugan R. , Akash B. , Abitha S. , Nandhamurugan R.
Published: April 2024

Background: The escalating prevalence of obesity is a significant health concern, closely associated with non-communicable diseases like diabetes, hypertension, dyslipidemia, and coronary heart disease. Research indicates that apple cider vinegar (ACV) may aid in lowering blood glucose levels, total cholesterol, triglycerides, body weight, waist circumference, and BMI, as demonstrated in animal studies and clinical trials. The primary objective of the study is to assess the impact of apple cider vinegar effervescent tablets on overweight and obese individuals within a period of 30-days.
Methods: This observational study took place at the outpatient department of general medicine at Sudha Institute of Medical Science in Erode, involving 45 participants with abnormal weight. Participants consumed an apple cider vinegar effervescent tablet daily in the afternoon for 30 days, alongside mild exercise and a low-sugar diet recommendation. Anthropometric measurements and participant satisfaction data were collected on the 0th (baseline), 15th, and 30th days. Data analysis was conducted using Microsoft Excel, with descriptive statistics such as mean and percentage utilized.
Results: This study comprised 75.56% of participants aged between 18 and 40 years. 44.44% of participants fall into the overweight category. Additionally, 95.5% of participants expressed an interest in weight reduction. The results revealed that reductions of 1.13 cm in waist circumference, 1.07 cm in hip circumference, 1.46 kg in weight, and 0.58 kg/m² in BMI over the 30-day period. Following the 30-day consumption of ACV, improvements were observed in abnormal food intake and resolved issues such as digestion, appetite, constipation, cravings, and gas problems.
Conclusion: This study concluded that daily consumption of apple cider vinegar effervescent tablet has a positive impact on weight loss and alleviates issues related to digestion, appetite, constipation, cravings, and gas problems.
Effect of Tillage and Weed Management Practices on Growth and Yield of Green Gram [Vigna radiata (L.) Wilczek]
Authors: Indra S. , Innazent Anitrosa , Joseph P. A. , B Balaganesh , Kumar P. Dinesh
Published: April 2024

A field experiment was conducted during 2023 at Karunya Institute of Technology and Sciences, Coimbatore, Tamil Nadu, to find out the effect of different tillage management [Minimum tillage (primary tillage only) and Conventional tillage (primary and secondary tillage)] and five weed control treatments [(PE Pendimethalin @ 1.0 kg ha-1, PE Pendimethalin + Imazethapyr (pre-mix) - Valor 32% EC 1.0 kg ha-1, Hand weeding on 15 and 30 DAS, Weed free plot and Un weeded control (weedy check)] on weeds and productivity of green gram (Vigna radiata (L.) Wilczek) during the winter (Rabi). Conventional tillage generally led to taller plants and higher leaf counts compared to minimum tillage, while effective weed management practices, particularly the combination of Pendimethalin and Imazethapyr, resulted in superior growth indices. Additionally, conventional tillage and weed-free conditions significantly enhanced dry matter production and yield parameters of green gram.
Influence of Sulphur and Zinc on Growth, Yield and Economics of Groundnut (Arachis hypogea L.)
Authors: Pawan . , Kumar Prateek , Mehera Biswarup , Vishal .
Published: April 2024

A field trial was led during Zaid (summer) season of 2023 at Crop Research Farm Department of Agronomy. The treatments contained of 3 level of Sulphur (15, 30 & 45 kg/ha) and 3 level of zinc (20kg/ha, 0.50% and 0.25% foliar application) along with recommended dosages of N, P, K and a control (20:60:40 NPK kg/ha). The investigate was laid out in a RBD with ten treatments and replication thrice. Application of Sulphur (45 kg/ha) + Zinc (10kg/ha + 0.25% foliar application) (treatment 9) recorded maximum plant height (46.00 cm), & yield attributes namely more number of pod/plants (26.53), highest seed yield (2.65 t/ha) and haulm yield (6.37 t/ha). The treatment also recorded, net return (135076 INR/ha) and B:C ratio (2.61).
Enhancing Wheat Yield and Economic Viability in Subtropical Rainfed Agriculture through Combined Herbicide Application
Authors: Kasi Indra Kumar , Charitha N. , Tiwari Awanindra Kumar , Gomadhi G. , Sahu Devendra Kumar , Devi Okram Ricky , Katiyar Drishty , Pandey Saurabh Raj , Afreen Nadiya
Published: April 2024

The management of weeds remains a critical aspect of agricultural production, particularly in rainfed subtropical regions where weed pressure can significantly impact crop yield and economic returns. This study aimed to evaluate the effects of combined herbicide applications on both the yield and economic viability of wheat (Triticum aestivum L.) in subtropical rainfed agricultural systems. Field trials were conducted over two consecutive growing seasons, employing a randomized complete block design with four treatments: (1) pre-emergence application of herbicide A, (2) pre-emergence application of herbicide B, (3) combined pre-emergence application of herbicides A and B, and (4) control (no herbicide application). The study assessed various parameters including weed density, wheat yield, and economic returns. Results indicated that the combined pre-emergence application of herbicides A and B significantly reduced weed density compared to individual herbicide applications and the control, consequently enhancing wheat yield. Furthermore, economic analysis revealed that the combined herbicide treatment resulted in the highest economic returns compared to individual herbicide applications and the control, indicating its economic viability in rainfed wheat cultivation in subtropical regions. These findings underscore the importance of integrated weed management strategies, particularly the judicious use of combined herbicide applications, for optimizing wheat yield and economic sustainability in subtropical rainfed agriculture.
Characterization and Evaluation of Native Rhizobium of Groundnut (Arachis hypogaea L.) and Soybean (Glycine max L.)
Authors: Chandrakanta . , Baghel Dayashankar , Gupta S.B. , Chowdhury Tapas
Published: April 2024

The present investigation was conducted to characterize and evaluate native Rhizobium isolates of groundnut and soybean to enhance the Legume-Rhizobium symbiosis as well as increase sustainable crop production. In this connection, all the native isolates under study were characterized biochemically and tested with N-free sand culture in the glasshouse to select effective native Rhizobium isolates through proper screening. 10 isolates of each crop were collected from the culture collection bank of the Department of Agricultural Microbiology, Indira Gandhi Krishi Vishwavidyalaya Raipur (Chhattisgarh). Out of 10 isolates, 3 isolates of each crop were selected on the basis of their growth performance and the purity of the culture. The biochemical studies of selected isolates include starch hydrolysis, catalase, urease, citrate utilization, indole production, gelatine iron, and methyl red tests, which were further evaluated for nodulation and biological nitrogen fixation in groundnut and soybean under controlled conditions with four treatments for each crop and five replications. The experiment result revealed that the evaluation of native Rhizobium isolate no. 1185 of groundnut and isolate no. 1076 of soybean was found to be superior among all the rhizobial isolates taken for study. These isolates accumulated 1.24, 1.44 mg/plant extra amount of atmospheric nitrogen over un-inoculated control plants, respectively. Further, it can be concluded that these native isolates may be the most effective nitrogen fixers for groundnut and soybean over the control.
Knowledge and Attitude of Mothers towards Human Papilloma Virus Vaccine Uptake in Nigeria
Authors: Meshach Osejele I. , Braimah Salami G. , David Uhomoibhi O. , Junior Omokaro E. , Ehis Ehizuelen G. , Stephen Abebe E. , Cyril Uangbaoje A. , Enobakhare Emmanuel E. , Martins Odafen , Precious Akhaine J.
Published: April 2024

Background: The human papilloma virus (HPV), a principal factor in the development of cervical cancer, is identified as one of the most widespread sexually transmitted infections, leading to a variety of conditions globally, from warts to cancer. It is estimated that 75% of people worldwide will contract an HPV infection at some point in their lives. Vaccination against HPV serves as an important preventive measure to reduce the rates of cervical cancer.
Objective: This study assessed knowledge, attitude of mothers toward HPV vaccine uptake in
Local communities in Edo State Nigeria.
Methods: A cross-sectional study utilizing a descriptive approach was carried out with 350 participants, chosen through simple random sampling. Information was gathered via questionnaires conducted by researchers and was processed with the Statistical Package for the Social Sciences (SPSS), version 25. To examine the relationships between variables, the Fisher's exact test was employed, with statistical significance determined by a P-value < 0.05.
Results: The average age of those surveyed was 40.4 years, with a standard deviation of 10.1 years. A mere 28.3% (44 respondents) had adequate knowledge about HPV and its vaccine. A significant portion (80%) exhibited a negative attitude toward receiving the HPV vaccine. There was a noticeable statistical correlation between the respondents' age and their awareness of HPV (with a p-value of 0.019) as well as between their employment status and their knowledge about HPV (p-value = 0.002). The primary reason cited by participants for not getting the HPV vaccine was its lack of availability.
Conclusion: Knowledge and uptake of HPV vaccination among the respondents were poor.
Majority also had negative attitude towards uptake of the vaccine.
Influence of Color Therapy in Alleviating Academic Stress in High School Students
Authors: Tiwari Ananya , Vijayvargiya Seema , Singh Bhupinder
Published: April 2024

Aim: Color Therapy as a powerful communication tool possesses the potential to impact emotions, moods, and psychological reactions. This study delves into the significance of colors and their influence on academic stress levels. The research aims to investigate the efficacy of color therapy, specifically utilizing blue and yellow, in alleviating academic stress among high school students.
Study Design: Purposive sampling method of research was used.
Place and Duration of Study: Saket MGM Senior Secondary School, Vidisha, near Bhopal (MP). The study took almost three months including data collection, one month 8 hours daily (excluding Saturday and Sunday) intervention.
Methodology: The sample comprised 150 high school students whose academic stress levels were initially measured by Academic Stress Scale by Kim. Subsequently, students exhibiting moderate to extreme stress levels were exposed to a color intervention and again the stress level was observed. The data was analyzed through Student’s t-test.
Results: The findings of the study revealed a substantial reduction in academic stress levels among students after exposure to blue and yellow color therapy. The accompanying statistical analysis, with a significant p-value of less than 0.01, underlines the robust evidence of this positive change.
Conclusion: The results suggest that employing specific colors in educational settings may offer a viable and non-intrusive approach to addressing academic stress among high school students. Implications of the study are discussed.
Protected Vegetable Crop Production for Long-term Sustainable Food Security
Authors: Chamuah Suchibrata , Kiran . , N Divyashree , Amin Md Al , Sultana Nazmin , Hansda Narendra Nath , B M Harish , Noopur Kohima
Published: April 2024

A protected culture strategy that offers a fully regulated environment decreases a number of biotic and abiotic stressors. Thinking about protected farming is necessary given the rising food need. The most effective way for meeting the goals of protected horticulture is the greenhouse. The cultivation of many greenhouses has become an important Indian agricultural policy. As a result of the greater productivity levels, these technologies are not only opening doors for producers with larger landholdings, but also for those with smaller holdings. Protected agriculture has provided a new way to produce more in a small space as a result of land holding restrictions, fast urbanisation, falling crop yield, declining biodiversity, and an ever-increasing population. Demand for food, particularly vegetables, has increased significantly as a result of these factors.
O Impacto da Indicação Geográfica no Registro de Marcas: um estudo sobre a cachaça
Authors: Santos Ana Cristina , Costa Patrícia Marinho , Aveni Alessandro
Published: April 2024

Ao considerar a Lei de Propriedade Intelectual, tanto o registro de marca quanto uma Indicação Geográfica conferem valor agregado a um produto ou processo, pois são modalidades de proteção intelectual. Como tal, essas modalidades podem ser incorporadas em uma negociação para melhorar a competitividade de determinado setor da economia. Algumas políticas públicas podem regulamentar essas questões com o propósito de melhorar a competitividade, mas é preciso que se observe o impacto dessas estratégias nos negócios brasileiros. Este trabalho apresenta uma discussão a respeito da proteção do produto cachaça no que se refere à indicação geográfica com base no arcabouço legal acerca do tema e sua relação com o registro de marcas. A pesquisa aprofundou-se nos dados quantitativos de pedido para registros de marcas e foi utilizada com objetivo de apresentar a evolução desses registros, no período de 2002 a 2022, com observações sobre deferimento/indeferimento. Por último, foi realizada uma avaliação do conflito entre a base legal para a Indicação Geográfica da cachaça e os registros das marcas desse destilado.
Prospecção Tecnológica de Patentes do Uso do Zebrafish em Substituição aos Animais de Laboratório
Authors: Milewski Christoph Schweitzer , Longa Leila  Costa Duarte , Barroso Wanise Borges Gouvea
Published: April 2024

O presente estudo verifica o potencial de utilização do zebrafish em substituição ao uso de animais de laboratório a partir da prospecção tecnológica de patentes. Esse biomodelo tem sido amplamente reconhecido em publicações como uma alternativa promissora em diversas áreas de pesquisa. O estudo foi realizado em 2023, em um período de duas décadas: 1) identificação de informações sobre zebrafish; 2) busca de patentes utilizando Questel e Cortellis plataformas; e 3) análise, compilação dos resultados e formulação de conclusões. A análise encontrou 1.681 famílias de patentes. Os resultados destacam que a utilização, a manutenção e a criação do zebrafish podem oferecer benefícios no desenvolvimento de pesquisas na área biomédica, quando comparados com outras opções de animais de laboratório, resultando em um custo-benefício mais favorável. O zebrafish possui semelhança genética de aproximadamente 70% com os seres humanos. O estudo evidencia a viabilidade do zebrafish em testes relacionados à pesquisa de forma concreta.
Prospecção Tecnológica sobre o Uso de Bagaço de Cana-de-Açúcar e seus Derivados para a Adsorção Seletiva e Compostos Contendo Enxofre no Diesel
Authors: Silva Ricardo Miguel Gonçalves da , Braga Patricia Regina Sobral , Costa Andréia  Alves
Published: April 2024

O objetivo desta pesquisa foi realizar uma prospecção tecnológica sobre o uso de bagaço de cana-de-açúcar e seus derivados para a adsorção seletiva de compostos contendo enxofre no diesel. A pesquisa se baseou na análise de documentos científicos provenientes das bases de dados Scopus e Web of Science, bem como na identificação de pedidos de patentes por meio da ferramenta Questel Orbit Intelligence®. Os resultados indicaram que os derivados do bagaço de cana-de-açúcar são adsorventes promissores, podendo ser aplicados na remoção de diversos compostos químicos presentes principalmente em águas residuais. No entanto, o uso no tratamento de combustíveis ainda não é uma aplicação amplamente desenvolvida.
Controlling the Thickness of the Surface Oxide Layer on Cu Nanoparticles for the Fabrication of Conductive Structures by Ink‐Jet Printing
Authors: Jeong Sunho , Woo Kyoohee , Kim Dongjo , Lim Soonkwon , Kim Jang Sub , Shin Hyunjung , Xia Younan , Moon Jooho
Published: February 2008

With the aim of preparing a high performance conductive ink, we sought to control the surface chemistry of Cu nanoparticles so as to minimize surface oxidation. Specifically, the surface oxide layer on Cu nanoparticles synthesized in ambient atmosphere was minimized by adjusting the molecular weight of poly(N‐vinylpyrrolidone) capping molecules, as confirmed by high resolution transmission electron microscopy and X‐ray photoelectron spectroscopy analyses. In addition, we demonstrate that by minimizing the thickness of the surface oxide layer, Cu granular films with good conductivity could be obtained by sintering nanoparticle assembles. Finally, we fabricated highly conductive Cu patterns on a plastic substrate by ink‐jet printing.
Synthesis of 2,3,4‐Trisubstituted Thiochromanes using an Organocatalytic Enantioselective Tandem Michael–Henry Reaction
Authors: Dodda Rajasekhar , Goldman Joshua J. , Mandal Tanmay , Zhao Cong‐Gui , Broker Grant A. , Tiekink Edward R. T.
Published: February 2008

Enantioenriched 2,3,4‐trisubstituted thiochromanes have been synthesized by using a cupreine‐catalyzed tandem Michael addition–Henry reaction between 2‐mercaptobenzaldehydes and β‐nitrostyrenes. Good diastereoselectivities and enantioselectivities were obtained for the title compounds, which may be further improved through a single recrystallization (up to 98% de and>99% ee).
Recyclable Carbon Supported Copper‐Manganese Oxide for Selective Aerobic Oxidation of Alcohols in Combination with 2,2,6,6‐Tetramethylpiperidyl‐1‐oxyl under Neutral Condition
Authors: Yang Guanyu , Zhu Weimin , Zhang Panke , Xue Huazhen , Wang Wei , Tian Junshan , Song Maoping
Published: February 2008

Due to the promotion of the surface area and the dispersion of active components upon supporting mixed metal oxides on the porous material active carbon, the copper‐manganese oxide on carbon system has been proven to be much more efficient than the co‐precipitation prepared Cu‐Mn oxide in mediating the 2,2,6,6‐tetramethylpiperidyl‐1‐oxyl (TEMPO)‐catalyzed aerobic oxidation of alcohols. Even at 30 °C and with a 0.1 mol% load of TEMPO, the oxidations proceeded smoothly. Upon catalysis with the Cu‐Mn oxide/C (10 wt%) and TEMPO (0.5–5 mol%), various alcohols were oxidized selectively to the corresponding aldehydes or ketones with molecular oxygen at 80 °C. Such a stable, recyclable heterogeneous cocatalyst permits alcohols to be oxidized under neutral and mild conditions.
Novel Chiral Biheteroaromatic Diphosphine Oxides for Lewis Base Activation of Lewis Acids in Enantioselective Allylation and Epoxide Opening
Authors: Simonini Valentina , Benaglia Maurizio , Benincori Tiziana
Published: February 2008

Enantiomerically pure biheteroaromatic diphosphine oxides were synthesised and tested as organocatalysts in two different reactions involving trichlorosilyl compounds. The allylation of aldehydes with allyl(trichloro)silane afforded homoallylic alcohols in fair to good yields and up to 95% ee. Preliminary experiments showed that this new class of metal‐free catalysts was able also to promote the stilbene oxide opening by addition of tetrachlorosilane with enantioselectivity higher than 80%. The interesting results in terms of chemical and stereochemical efficiency open the way to further studies towards the development of new chiral heteroaromatic Lewis bases as efficient metal‐free catalysts.