bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficient Phosphorus Recovery from Municipal Wastewater Using Enhanced Biological Phosphorus Removal in an Anaerobic/Anoxic/Aerobic Membrane Bioreactor and Magnesium-Based Pellets
Authors: Eghombi Elvis , Kim Hyunsik , Choi Yang-Hun , Baek Mi-Hwa , Nadagouda Mallikarjuna N. , Park Pyung-Kyu , Chae Soryong
Published: February 2022

Municipal wastewater has been identified as a potential source of natural phosphorus (P) that is projected to become depleted in a few decades based on current exploitation rates. This paper focuses on combining a bench-scale anaerobic/anoxic/aerobic membrane bioreactor (MBR) and magnesium carbonate (MgCO3)-based pellets to effectively recover P from municipal wastewater. Ethanol was introduced into the anoxic zone of the MBR system as an external carbon source to improve P release via the enhanced biological phosphorus removal (EBPR) mechanism, making it available for adsorption by the continuous-flow MgCO3 pellet column. An increase in the concentration of P in the MBR effluent led to an increase in the P adsorption capacity of the MgCO3 pellets. As a result, the anaerobic/anoxic/aerobic MBR system, combined with a MgCO3 pellet column and ethanol, achieved 91.6% P recovery from municipal wastewater, resulting in a maximum P adsorption capacity of 12.8 mg P/g MgCO3 through the continuous-flow MgCO3 pellet column. Although the introduction of ethanol into the anoxic zone was instrumental in releasing P through the EBPR, it could potentially increase membrane fouling by increasing the concentration of extracellular polymeric substances (EPSs) in the anoxic zone.
Tissue Inhibitor of Metalloproteases 3 (TIMP-3): In Vivo Analysis Underpins Its Role as a Master Regulator of Ectodomain Shedding
Authors: Spanò Donatella , Scilabra Simone
Published: February 2022

The proteolytical cleavage of transmembrane proteins with subsequent release of their extracellular domain, so-called ectodomain shedding, is a post-translational modification that plays an essential role in several biological processes, such as cell communication, adhesion and migration. Metalloproteases are major proteases in ectodomain shedding, especially the disintegrin metalloproteases (ADAMs) and the membrane-type matrix metalloproteases (MT-MMPs), which are considered to be canonical sheddases for their membrane-anchored topology and for the large number of proteins that they can release. The unique ability of TIMP-3 to inhibit different families of metalloproteases, including the canonical sheddases (ADAMs and MT-MMPs), renders it a master regulator of ectodomain shedding. This review provides an overview of the different functions of TIMP-3 in health and disease, with a major focus on the functional consequences in vivo related to its ability to control ectodomain shedding. Furthermore, herein we describe a collection of mass spectrometry-based approaches that have been used in recent years to identify new functions of sheddases and TIMP-3. These methods may be used in the future to elucidate the pathological mechanisms triggered by the Sorsby’s fundus dystrophy variants of TIMP-3 or to identify proteins released by less well characterized TIMP-3 target sheddases whose substrate repertoire is still limited, thus providing novel insights into the physiological and pathological functions of the inhibitor.
Assessment of Exposure to Di-(2-ethylhexyl) Phthalate (DEHP) Metabolites and Bisphenol A (BPA) and Its Importance for the Prevention of Cardiometabolic Diseases
Authors: Carli Fabrizia , Ciociaro Demetrio , Gastaldelli Amalia
Published: February 2022

Exposomics analyses have highlighted the importance of biomonitoring of human exposure to pollutants, even non-persistent, for the prevention of non-communicable diseases such as obesity, diabetes, non-alcoholic fatty liver disease, atherosclerosis, and cardiovascular diseases. Phthalates and bisphenol A (BPA) are endocrine disrupting chemicals (EDCs) widely used in industry and in a large range of daily life products that increase the risk of endocrine and cardiometabolic diseases especially if the exposure starts during childhood. Thus, biomonitoring of exposure to these compounds is important not only in adulthood but also in childhood. This was the goal of the LIFE-PERSUADED project that measured the exposure to phthalates (DEHP metabolites, MEHP, MEHHP, MEOHP) and BPA in Italian mother–children couples of different ages. In this paper we describe the method that was set up for the LIFE PERSUADED project and validated during the proficiency test (ICI/EQUAS) showing that accurate determination of urinary phthalates and BPA can be achieved starting from small sample size (0.5 mL) using two MS techniques applied in cascade on the same deconjugated matrix.
Virulence Regulation and Drug-Resistance Mechanism of Fungal Infection
Authors: Angiolella Letizia
Published: February 2022

In this issue, I propose promising developments in the field of the mechanism of virulence factors that can be linked to antifungal resistance [...]
Interactions of Vallisneria natans and Iron-Oxidizing Bacteria Enhance Iron-Bound Phosphorus Formation in Eutrophic Lake Sediments
Authors: Wang Juanjuan , Gao Mingming , Yang Yanju , Lu Shipeng , Wang Guiliang , Qian Xiaoqing
Published: February 2022

Submerged macrophyte restoration and in situ phosphorus (P) passivation are effective methods for the control of internal P loading from sediments. This study explored the synergistic effects of Vallisneria natans and iron (Fe)-oxidizing bacteria (IOB) on internal P loading from eutrophic freshwater lake sediments by taking into account Fe-bound P (FeP) formation and associated bacterial community structures. Sediment samples were prepared in glass tanks under four treatments, namely no V. natans planting or IOB inoculation (control), planting V. natans without IOB inoculation (Va), planting V. natans with IOB inoculation (Va-IOB), and planting V. natans with autoclaved IOB inoculation (Va-IOB[A]). Compared with the control, all three treatments with V. natans (Va, Va-IOB, and Va-IOB[A]) had significantly decreased organic matter contents and increased redox potential in sediments (p < 0.05), at the rapid growth and mature stages of V. natans. Planting V. natans with and without IOB inoculation also decreased the total P (TP) and Fe–P concentrations in sediments. Conversely, Fe3+ concentrations, Fe3+/Fe2+ ratios, and the proportions of Fe–P in TP all increased in sediments planted with V. natans, especially under the Va-IOB treatment (p < 0.05). Furthermore, bacterial community diversity increased in sediments due to the presence of V. natans. The relative abundances of IOB (including Acidovorax and Chlorobium) increased from the transplanting to the rapid growth stage of V. natans and then decreased afterwards. In the later stages, the relative abundances of IOB and their ratios to Fe-reducing bacteria were the highest under the Va-IOB treatment. Accordingly, synergistic interactions between V. natans and IOB could enhance Fe–P formation and reduce TP concentrations in eutrophic lake sediments by altering sediment physicochemical properties and Fe oxidation-related bacterial community structures.
Antiparasitic Ovalicin Derivatives from Pseudallescheria boydii, a Mutualistic Fungus of French Guiana Termites
Authors: Sorres Jonathan , Hebra Téo , Elie Nicolas , Leman-Loubière Charlotte , Grayfer Tatyana , Grellier Philippe , Touboul David , Stien Didier , Eparvier Véronique
Published: February 2022

Social insects are in mutualism with microorganisms, contributing to their resistance against infectious diseases. The fungus Pseudallescheria boydii SNB-CN85 isolated from termites produces ovalicin derivatives resulting from the esterification of the less hindered site of the ovalicin epoxide by long-chain fatty acids. Their structures were elucidated using spectroscopic analysis and semisynthesis from ovalicin. For ovalicin, these compounds displayed antiprotozoal activities against Plasmodium falciparum and Trypanosoma brucei, with IC50 values of 19.8 and 1.1 µM, respectively, for the most active compound, i.e., ovalicin linoleate. In parallel, metabolomic profiling of a collection of P. boydii strains associated with termites made it possible to highlight this class of compounds together with tyroscherin derivatives in all strains. Finally, the complete genome of P. boydii strains was obtained by sequencing, and the cluster of potential ovalicin and ovalicin biosynthesis genes was annotated. Through these metabolomic and genomic analyses, a new ovalicin derivative named boyden C, in which the 6-membered ring of ovalicin was opened by oxidative cleavage, was isolated and structurally characterized.
Storage Stability and In Vitro Bioaccessibility of Liposomal Betacyanins from Red Pitaya (Hylocereus polyrhizus)
Authors: Lin Xian , Li Bozhe , Wen Jing , Wu Jijun , Tang Daobang , Yu Yuanshan , Xu Yujuan , Xu Baojun
Published: February 2022

In order to address the poor stability of the betacyanins from red pitaya (Hylocereus polyrhizus, HP), which are considered as good sources of natural colorant, liposomal-encapsulation technique was applied in this study. Thin-layer dispersion method was employed to prepare HP betacyacnin liposomes (HPBL). The formulation parameters for HPBL were optimized, and the characteristics, stability, and release profile of HPBL in in vitro gastrointestinal systems were evaluated.Results showed that an HP betacyanin encapsulation efficiency of 93.43 ± 0.11% was obtained after formulation optimization. The HPBL exhibited a narrow size distribution of particle within a nanometer range and a strong electronegative ζ-potential. By liposomal encapsulation, storage stability of HP betacyanin was significantly enhanced in different storage temperatures. When the environmental pH ranged from 4.3–7.0, around 80% of HP betacyanins were preserved on Day 21 with the liposomal protection. The loss of 2,2′-Diphenyl-picrylhydrazyl (DPPH) scavenging activity and color deterioration of HPBL were developed in accordance with the degradation of HP betacyanins during storage. In in vitro gastrointestinal digestion study, with the protection of liposome, the retention rates of HP betacyanins in vitro were enhanced by 14% and 40% for gastric and intestinal digestion, respectively.This study suggested that liposomal encapsulation was an effective approach to stabilize HP betacyanins during storage and gastrointestinal digestion, but further investigations were needed to better optimize the liposomal formulation and understand the complex liposomal system.
Bioactive Compounds from Pale Ale Beer Powder Attenuate Experimental Colitis in BALB/c Mice
Authors: Maia Paola D. D. S. , Baião Diego dos Santos , Nanini Hayandra F. , da Silva Victor Paulo F. , Frambach Lissa Bantim , Cabral Iuri Matheus , Pêgo Beatriz , Ribeiro Beatriz E. , Pavão Mauro Sérgio Gonçalves , Paschoalin Vania M. F. , de Souza Heitor S. P. , Pierucci Anna Paola T. R.
Published: February 2022

Phenolic compounds (PCs) present in foods are associated with a decreased risk of developing inflammatory diseases. The aim of this study was to extract and characterize PCs from craft beer powder and evaluate their potential benefits in an experimental model of inflammatory bowel disease (IBD). PCs were extracted and quantified from pure beer samples. BALB/c mice received either the beer phenolic extract (BPE) or beer powder fortified with phenolic extract (BPFPE) of PCs daily for 20 days by gavage. Colon samples were collected for histopathological and immunohistochemical analyses. Dextran sodium sulfate (DSS)-induced mice lost more weight, had reduced colon length, and developed more inflammatory changes compared with DSS-induced mice treated with either BPE or BPFPE. In addition, in DSS-induced mice, the densities of CD4- and CD11b-positive cells, apoptotic rates, and activation of NF-κB and p-ERK1/2 MAPK intracellular signaling pathways were higher in those treated with BPE and BPFPE than in those not treated. Pretreatment with the phenolic extract and BPFPE remarkably attenuated DSS-induced colitis. The protective effect of PCs supports further investigation and development of therapies for human IBD.
Effect of Different Sterilization Methods on the Microbial and Physicochemical Changes in Prunus mume Juice during Storage
Authors: Ma Yuan , Xu Yingping , Chen Yuanyuan , Meng Ailian , Liu Ping , Ye Kunyue , Yuan Anqi
Published: February 2022

This study evaluated the pasteurization (P), ozone (O3), ultrasonic (US), and high-hydrostatic-pressure (HHP) sterilization approaches for processing of Prunus mume regarding browning factors and microorganisms, compared with non-sterilization (control check, CK) treatment. The microorganisms (total bacterial count and fungi and yeast count) in the juice were identified after different sterilization techniques, while the quality parameter changes (degree of browning, color measurements, total phenolic content, reducing sugar, ascorbic acid, 5-hydroxymethyl furaldehyde (5-HMF), amino acid nitrogen, total soluble solids (TSS), pH value) were investigated. The results indicate that P and HHP treatment reduced non-enzymatic browning while substantially impacting the color measurements, TSS, and pH, while the sterilization effect was remarkable, with a rate exceeding 90%. Furthermore, the Prunus mume juices treated with P and HHP sterilization were used as the objects, and the CK group was used as the control group. They were placed at 4 °C, 25 °C and 37 °C, respectively, and stored in dark for 15 d. Sampling and determination were carried out on 0, 3, 6, 9, 12, and 15 d, respectively. M-&-Y (molds and yeasts) were not detected in the late storage period, and no obvious microbial growth was observed during storage, indicating that P and HHP treatments could ensure the microbial safety of Prunus mume juice. P- and HHP- treated Prunus mume juice has better quality and low temperature storage is beneficial for maintaining the quality of Prunus mume juice. Therefore, P treatment or HHP treatment combined with low temperature storage could achieve a more ideal storage effect. Overall, this study conclusively established that P and HHP methods were suitable for sterilizing Prunus mume juice. These techniques minimally affected overall product quality while better maintaining the quality parameters than the untreated juice samples and those exposed to O3 and US treatment.
Pharmacological Activities of Soursop (Annona muricata Lin.)
Authors: Mutakin Mutakin , Fauziati Rizky , Fadhilah Fahrina Nur , Zuhrotun Ade , Amalia Riezki , Hadisaputri Yuni Elsa
Published: February 2022

Soursop (Annona muricata Lin.) is a plant belonging to the Annonaceae family that has been widely used globally as a traditional medicine for many diseases. In this review, we discuss the traditional use, chemical content, and pharmacological activities of A.muricata. From 49 research articles that were obtained from 1981 to 2021, A.muricata’s activities were shown to include anticancer (25%), antiulcer (17%), antidiabetic (14%), antiprotozoal (10%), antidiarrhea (8%), antibacterial (8%), antiviral (8%), antihypertensive (6%), and wound healing (4%). Several biological activities and the general mechanisms underlying the effects of A.muricata have been tested both in vitro and in vivo. A.muricata contains chemicals such as acetogenins (annomuricins and annonacin), alkaloids (coreximine and reticuline), flavonoids (quercetin), and vitamins, which are predicted to be responsible for the biological activity of A.muricata.
Piceatannol Protects Brain Endothelial Cell Line (bEnd.3) against Lipopolysaccharide-Induced Inflammation and Oxidative Stress
Authors: Zhou Yan , Khan Haroon , Hoi Maggie Pui Man , Cheang Wai San
Published: February 2022

Dysfunction of the blood–brain barrier (BBB) is involved in the pathogenesis of many cerebral diseases. Oxidative stress and inflammation are contributing factors for BBB injury. Piceatannol, a natural ingredient found in various plants, such as grapes, white tea, and passion fruit, plays an important role in antioxidant and anti-inflammatory responses. In this study, we examined the protective effects of piceatannol on lipopolysaccharide (LPS) insult in mouse brain endothelial cell line (bEnd.3) cells and the underlying mechanisms. The results showed that piceatannol mitigated the upregulated expression of adhesion molecules (ICAM-1 and VCAM-1) and iNOS in LPS-treated bEnd.3 cells. Moreover, piceatannol prevented the generation of reactive oxygen species in bEnd.3 cells stimulated with LPS. Mechanism investigations suggested that piceatannol inhibited NF-κB and MAPK activation. Taken together, these observations suggest that piceatannol reduces inflammation and oxidative stress through inactivating the NF-κB and MAPK signaling pathways on cerebral endothelial cells in vitro.
The Current State of Knowledge on Salvia hispanica and Salviae hispanicae semen (Chia Seeds)
Authors: Motyka Sara , Koc Katarzyna , Ekiert Halina , Blicharska Eliza , Czarnek Katarzyna , Szopa Agnieszka
Published: February 2022

Chia seeds (Salviae hispanicae semen) are obtained from Salvia hispanica L. This raw material is distinguished by its rich chemical composition and valuable nutritional properties. It is currently referred to as “health food”. The purpose of the present work was to perform a literature review on S. hispanica and chia seeds, focusing on their chemical composition, biological properties, dietary importance, and medicinal uses. The valuable biological properties of chia seeds are related to their rich chemical composition, with particularly high content of polyunsaturated fatty acids, essential amino acids, polyphenols, as well as vitamins and bioelements. The available scientific literature indicates the cardioprotective, hypotensive, antidiabetic, and antiatherosclerotic effects of this raw material. In addition, studies based on in vitro assays and animal and human models have proven that chia seeds are characterized by neuroprotective, hepatoprotective, anti-inflammatory, and antioxidant properties. These properties indicate a valuable role of chia in the prevention of civilization diseases. Chia seeds are increasingly popular in functional food and cosmetic and pharmaceutical industries. That is attributed not only to their desirable chemical composition and biological activity but also to their high availability. Nevertheless, S. hispanica is also the object of specific biotechnological studies aimed at elaboration of micropropagation protocols of this plant species.
Resource-Conserving Protection against Energy Draining (RCPED) Routing Protocol for Wireless Sensor Networks
Authors: Gong Pu , Chen Thomas M. , Xu Peng
Published: February 2022

This paper proposes a routing protocol for wireless sensor networks to deal with energy-depleting vampire attacks. This resource-conserving protection against energy-draining (RCPED) protocol is compatible with existing routing protocols to detect abnormal signs of vampire attacks and identify potential attackers. It responds to attacks by selecting routes with the maximum priority, where priority is an indicator of energy efficiency and estimation of security level calculated utilizing an analytic hierarchy process (AHP). RCPED has no dependence on cryptography, which consumes less energy and hardware resources than previous approaches. Simulation results show the benefits of RCPED in terms of energy efficiency and security awareness.
Stability and Transformation of Metabolic Syndrome in Adolescents: A Prospective Assessment in Relation to the Change of Cardiometabolic Risk Factors
Authors: Wu Pei-Wen , Lai Yi-Wen , Chin Yu-Ting , Tsai Sharon , Yang Tun-Min , Lin Wei-Ting , Lee Chun-Ying , Tsai Wei-Chung , Huang Hsiao-Ling , Seal David W. , Duh Tsai-Hui , Lee Chien-Hung
Published: February 2022

Underlying pathophysiological mechanisms drive excessive clustering of cardiometabolic risk factors, causing metabolic syndrome (MetS). MetS status may transform as adolescents transition to young adulthood. This study investigated the latent clustering structure and its stability for MetS during adolescence, and assessed the anthropometric and clinical metabolic determinants for MetS transformation. A community-based representative adolescent cohort (n = 1516) was evaluated for MetS using four diagnostic criteria, and was followed for 2.2 years to identify new-onset MetS. The clustering structure underlying cardiometabolic parameters was stable across adolescence; both comprised a fat—blood pressure (BP)—glucose three-factor structure (total variance explained: 68.8% and 69.7% at baseline and follow-up, respectively). Among adolescents with MetS-negative at baseline, 3.2–4.4% had incident MetS after 2.2 years. Among adolescents with MetS-positive at baseline, 52.0–61.9% experienced MetS remission, and 38.1–48.0% experienced MetS persistence. Increased systolic BP (SBP) was associated with a high MetS incidence risk, while decreased levels of SBP and glucose were associated with MetS remission. Compared with adolescents with a normal metabolic status at baseline, those with an initial abdominal obesity and increased triglycerides level had a 15.0- and 5.7-fold greater risk for persistent abnormality, respectively. Abdominal obesity and low high-density lipoprotein cholesterol are two abnormal MetS components that highly persist during adolescence, and are the intervention targets for reducing the future risk of cardiometabolic disorders.
Combined Evaluation of Geriatric Nutritional Risk Index and Modified Creatinine Index for Predicting Mortality in Patients on Hemodialysis
Authors: Yajima Takahiro , Yajima Kumiko , Arao Maiko
Published: February 2022

The geriatric nutritional risk index (GNRI) and modified creatinine index (mCI) are surrogate markers of protein-energy wasting in patients receiving hemodialysis. We aimed to examine whether a combined evaluation of these indices improved mortality prediction in this population. We retrospectively investigated 263 hemodialysis patients divided into two groups, using 91.2 and 20.16 mg/kg/day as cut-off values of GNRI and mCI, respectively. The resultant four groups were reshuffled into four subgroups defined using combinations of cut-off values of both indices and were followed up. During the follow-up period (median: 3.1 years), 103 patients died (46/103, cardiovascular causes). Lower GNRI and lower mCI were independently associated with all-cause mortality (adjusted hazard ratio (aHR) 4.96, 95% confidence intervals (CI) 3.10–7.94, and aHR 1.92, 95% CI 1.22–3.02, respectively). The aHR value for the lower GNRI and lower mCI group vs. the higher GNRI and higher mCI group was 7.95 (95% CI 4.38–14.43). Further, the addition of GNRI and mCI to the baseline risk assessment model significantly improved the C-index of all-cause mortality (0.801 to 0.835, p = 0.025). The simultaneous evaluation of GNRI and mCI could be clinically useful to stratify the risk of mortality and to improve the predictability of mortality in patients on hemodialysis.
The Role of Shared Decision-Making in Personalised Medicine: Opening the Debate
Authors: Guadalajara Hector , Lopez-Fernandez Olatz , León Arellano Miguel , Domínguez-Prieto Víctor , Caramés Cristina , Garcia-Olmo Damian
Published: February 2022

Surgeons and cancer patients are starting to open the debate on how personalised medicine could use shared decision-making (SDM) to balance the personal and clinical components and thus improve the quality and value of care. Personalised precision medicine (PPM) has traditionally focused on the use of genomic information when prescribing treatments, which are usually pharmaceutical. However, the knowledge base is considerably scarcer in terms of how clinicians can individualise the information they provide patients about the consequences of different treatments, and in doing so involve them in the decision-making process. To achieve this, the ethical implications of SDM must be addressed from both sides. This paper explores the medical characteristics, the SDM implications in severe and fragile patients, potential risks, and observed benefits within this healthcare approach through four clinical cases. Findings shed light on current needs for clinician and patient training and tools related to SDM in PPM, and also remarks on the way in which this shift in healthcare settings is taking place to include the human component together with the biological and technological advances when designing care processes in colorectal cancer.
Identification of a Quinone Derivative as a YAP/TEAD Activity Modulator from a Repurposing Library
Authors: Lauriola Angela , Uliassi Elisa , Santucci Matteo , Bolognesi Maria Laura , Mor Marco , Scalvini Laura , Elisi Gian Marco , Gozzi Gaia , Tagliazucchi Lorenzo , Marverti Gaetano , Ferrari Stefania , Losi Lorena , D’Arca Domenico , Costi Maria Paola
Published: February 2022

The transcriptional regulators YAP (Yes-associated protein) and TAZ (transcriptional co-activator with PDZ-binding motif) are the major downstream effectors in the Hippo pathway and are involved in cancer progression through modulation of the activity of TEAD (transcriptional enhanced associate domain) transcription factors. To exploit the advantages of drug repurposing in the search of new drugs, we developed a similar approach for the identification of new hits interfering with TEAD target gene expression. In our study, a 27-member in-house library was assembled, characterized, and screened for its cancer cell growth inhibition effect. In a secondary luciferase-based assay, only seven compounds confirmed their specific involvement in TEAD activity. IA5 bearing a p-quinoid structure reduced the cytoplasmic level of phosphorylated YAP and the YAP–TEAD complex transcriptional activity and reduced cancer cell growth. IA5 is a promising hit compound for TEAD activity modulator development.
Linkers: An Assurance for Controlled Delivery of Antibody-Drug Conjugate
Authors: Sheyi Rotimi , de la Torre Beatriz G. , Albericio Fernando
Published: February 2022

As one of the major therapeutic options for cancer treatment, chemotherapy has limited selectivity against cancer cells. Consequently, this therapeutic strategy offers a small therapeutic window with potentially high toxicity and thus limited efficacy of doses that can be tolerated by patients. Antibody-drug conjugates (ADCs) are an emerging class of anti-cancer therapeutic drugs that can deliver highly cytotoxic molecules directly to cancer cells. To date, twelve ADCs have received market approval, with several others in clinical stages. ADCs have become a powerful class of therapeutic agents in oncology and hematology. ADCs consist of recombinant monoclonal antibodies that are covalently bound to cytotoxic chemicals via synthetic linkers. The linker has a key role in ADC outcomes because its characteristics substantially impact the therapeutic index efficacy and pharmacokinetics of these drugs. Stable linkers and ADCs can maintain antibody concentration in blood circulation, and they do not release the cytotoxic drug before it reaches its target, thus resulting in minimum off-target effects. The linkers used in ADC development can be classified as cleavable and non-cleavable. The former, in turn, can be grouped into three types: hydrazone, disulfide, or peptide linkers. In this review, we highlight the various linkers used in ADC development and their design strategy, release mechanisms, and future perspectives.
A Convenient and Simple Ionic Polymer-Metal Composite (IPMC) Actuator Based on a Platinum-Coated Sulfonated Poly(ether ether ketone)–Polyaniline Composite Membrane
Authors: Luqman Mohammad , Shaikh Hamid M. , Anis Arfat , Al-Zahrani Saeed M. , Alam Mohammad Asif
Published: February 2022

Herein, we present new approaches for developing sulfonated polyether ether ketone (SPEEK) and polyaniline-based (PANI) actuator formed by film-casting and chemical reduction of Pt electrodes. We have thoroughly studied the synthesis of SPEEK and characterized it by different analytical techniques. The ion-exchange capacity (IEC) and proton conductivity of SPEEK-PANI polymer membrane were calculated to be 1.98 mmol g−1 and 1.97 × 10−3 S cm−1, respectively. To develop an IPMC actuator, SPEEK was combined with PANI through in-situ polymerization method. SEM and XRD were used to check the morphology of the given SPEEK-PANI-Pt membrane. In addition, FT-IR and EDX techniques confirmed the molecular structure and chemical conformation of SPEEK-PANI polymer membrane. Pt electrode layers homogeneously dispersed on the IPMC membrane surface, which was demonstrated by smooth SEM micrographs. The actuation functioning, including the high bending deflection, proton conductivity, current density and IEC of IPMC actuator based on SPEEK-PANI-Pt, was obtained owing to its strong electrochemical and electromechanical characteristics. Synergistic combinations of SPEEK and PANI produced membrane that are flexible, mechanically strong and robust. The developed materials have immense capability as actuators for various applications including in biomimetics and robotics.
Quantitative and Qualitative Aspects of Composite Action of Concrete and Dispersion-Reinforcing Fiber
Authors: Stel’makh Sergey A. , Shcherban’ Evgenii M. , Beskopylny Alexey , Mailyan Levon R. , Meskhi Besarion , Varavka Valery
Published: February 2022

The interest in using polymer-dispersed reinforcement in the construction industry in the context of sustainability has led to significant research on this scientific problem. The article is devoted to studying the processes of fiber interaction depending on its dispersion and the concrete matrix, and their combined contact work during the formation of a concrete structure, work under stresses arising in a concrete body, and during a collapse. The physical and mechanical processes of deformation and destruction of the “matrix–fiber” system were studied using high-precision microscopic equipment, and the nature of the work and deformation of fibers in concrete were revealed. The work aimed to establish and characterize the quantitative and qualitative aspects of the concrete matrix and dispersion-reinforcing fiber combined work. It was established that the best values of the adhesion index were observed at a volume content of fiber in the amount of 2% by weight of cement, regardless of the type of dispersion-reinforcing fiber. It was shown that the microstructure of polydispersion-reinforced fiber-cement specimens was denser, and microcracks formed during fracture in polydispersion-reinforced specimens had a smaller opening width. It was established that polydispersion-reinforced concrete had higher values of strength (up to 126%) and deformation (up to 296%) characteristics compared to monodispersion fiber-reinforced concrete.
The Potential of the Oil-Producing Oleaginous Yeast Rhodotorula mucilaginosa for Sustainable Production of Bio-Oil Energy
Authors: Tsai Shu-Yao , Yu Hsuan-Ti , Lin Chun-Ping
Published: February 2022

At the 2021 United Nations Climate Change Conference (COP26), many countries in the world agreed to reach the goal of net-zero greenhouse gas emissions by 2050. This issue constrains energy use, petrochemical use, and related manufacturing production patterns. It is undeniable that the diesel engine of large equipment is still widely used in engineering applications, and it will not disappear in a short time. Many industrial projects still have to face the use of diesel engines. This study was focused on the development of oil-producing microorganisms to generate lipids. The oleaginous yeast Rhodotorula mucilaginosa (R. mucilaginosa) was selected for liquid-state cultivation, and the conditions for growth of the yeast cells were assessed. For the liquid fermentation culture with a fixed inoculation amount of 5%, it was determined that a suitable oil-producing culture was obtained on the sixth day, and the optimum conditions involved a carbon source concentration of 60 g/L, a yeast extract concentration of 0.5 g/L, and a KH2PO4 concentration of 7.0 g/L for each batch of culture experiments. In addition, the extraction method and solvent for the extraction of R. mucilaginosa lipids were chosen. The acid-heat method using the green organic solvent ethyl acetate exhibited the best performance for extraction of yeast lipids under environmentally friendly and safe conditions. The analysis of lipids showed that the fatty acids obtained primarily contained C16:0, C18:1 and C18:2, and especially C18:1 (41%) lipids, indicating that R. mucilaginosa lipids are a good bio-oil source for the production of biodiesel.
Assessment of Three Long-Term Satellite-Based Precipitation Estimates against Ground Observations for Drought Characterization in Northwestern China
Authors: Guo Hao , Li Min , Nzabarinda Vincent , Bao Anming , Meng Xiangchen , Zhu Li , De Maeyer Philippe
Published: February 2022

Long-term satellite-based precipitation estimates (LSPE) play a significant role in climatological studies like drought monitoring. In this study, three popular LSPEs (Precipitation Estimation from Remotely Sensed Information using Artificial Neural Networks–Climate Data Record (PERSIANN-CDR), Rainfall Estimates from Rain Gauge and Satellite Observations (CHIRPS) and Multi-Source Weighted-Ensemble Precipitation (MSWEP)) were evaluated on a monthly scale using ground-based stations for capturing drought event characteristics over northwestern China from 1983 to 2013. To reflect dry or wet evolution, the Standardized Precipitation Index (SPI) was adopted, and the Run theory was used to identify drought events and their characteristics. The conventional statistical indices (relative bias (RB), correlation coefficient (CC), and root mean square error (RMSE)), as well as categorical indices (probability of detection (POD), false alarm ratio (FAR), and missing ratio (MISS)) are used to evaluate the capability of LSPEs in estimating precipitation and drought characteristics. We found that: (1) three LSPEs showed generally satisfactory performance in estimating precipitation and characterizing drought events. Although LSPEs have acceptable performance in identifying drought events with POD greater than 60%, they still have a high false alarm ratio (>27%) and a high missing ratio (>33%); (2) three LSPEs tended to overestimate drought severity, mainly because of an overestimation of drought duration; (3) the ability of CHIRPS to replicate the temporal evolution of precipitation and SPI values is limited; (4) in severe drought events, PERSIANN-CDR tends to overestimate precipitation, and drought severity, as well as drought area; (5) among the three LSPEs, MSWEP outperformed the other two in identifying drought events (POD > 66%) and characterizing drought features. Finally, we recommend MSWEP for drought monitoring studies due to its high accuracy in estimating drought characteristics over northwestern China. In drought monitoring applications, the overestimation of PERSIANN-CDR for drought peak value and area, as well as CHIRPS’s inferiority in capturing drought temporal evolution, must be considered.
Mapping Key Indicators of Forest Restoration in the Amazon Using a Low-Cost Drone and Artificial Intelligence
Authors: Albuquerque Rafael Walter , Vieira Daniel Luis Mascia , Ferreira Manuel Eduardo , Soares Lucas Pedrosa , Olsen Søren Ingvor , Araujo Luciana Spinelli , Vicente Luiz Eduardo , Tymus Julio Ricardo Caetano , Balieiro Cintia Palheta , Matsumoto Marcelo Hiromiti , Grohmann Carlos Henrique
Published: February 2022

Monitoring the vegetation structure and species composition of forest restoration (FR) in the Brazilian Amazon is critical to ensuring its long-term benefits. Since remotely piloted aircrafts (RPAs) associated with deep learning (DL) are becoming powerful tools for vegetation monitoring, this study aims to use DL to automatically map individual crowns of Vismia (low resilience recovery indicator), Cecropia (fast recovery indicator), and trees in general (this study refers to individual crowns of all trees regardless of species as All Trees). Since All Trees can be accurately mapped, this study also aims to propose a tree crown heterogeneity index (TCHI), which estimates species diversity based on: the heterogeneity attributes/parameters of the RPA image inside the All Trees results; and the Shannon index measured by traditional fieldwork. Regarding the DL methods, this work evaluated the accuracy of the detection of individual objects, the quality of the delineation outlines and the area distribution. Except for Vismia delineation (IoU = 0.2), DL results presented accurate values in general, as F1 and IoU were always greater than 0.7 and 0.55, respectively, while Cecropia presented the most accurate results: F1 = 0.85 and IoU = 0.77. Since All Trees results were accurate, the TCHI was obtained through regression analysis between the canopy height model (CHM) heterogeneity attributes and the field plot data. Although TCHI presented robust parameters, such as p-value < 0.05, its results are considered preliminary because more data are needed to include different FR situations. Thus, the results of this work show that low-cost RPA has great potential for monitoring FR quality in the Amazon, because Vismia, Cecropia, and All Trees can be automatically mapped. Moreover, the TCHI preliminary results showed high potential in estimating species diversity. Future studies must assess domain adaptation methods for the DL results and different FR situations to improve the TCHI range of action.
Compact Midwave Imaging System: Results from an Airborne Demonstration
Authors: Kelly Michael A. , Carr James L. , Wu Dong L. , Goldberg Arnold C. , Papusha Ivan , Meinhold Renee T.
Published: February 2022

The Compact Midwave Imaging System (CMIS) is a wide field of view, multi-angle, multi-spectral pushframe imager that relies on the forward motion of the satellite to create a two-dimensional (2D) image swath. An airborne demonstration of CMIS was successfully completed in January–February 2021 on the NASA Langley Research Center Gulfstream III. The primary objective of the four-flight campaign was to demonstrate the capability of this unique instrument to perform stereo observations of clouds and other particulates (e.g., smoke) in the atmosphere. It is shown that the midwave infrared (MWIR) spectral bands of CMIS provide a unique 24/7 capability with high resolution for accurate stereo sensing. The instrument relies on new focal plane array (FPA) technology, which provides excellent sensitivity at much warmer detector temperatures than traditional technologies. This capability enabled a compact, low-cost design that can provide atmospheric motion vectors and cloud heights to support requirements for atmospheric winds in the 2017–2027 Earth Science Decadal Survey. Applications include day/night observations of the planetary boundary layer, severe weather, and wildfires. A comparison with current space-based earth science instruments demonstrates that the SWIR/MWIR multi-spectral capability of CMIS is competitive with larger, more expensive instrumentation. Imagery obtained over a controlled burn and operating nuclear power plant demonstrated the sensitivity of the instrument to temperature variations. The system relies on a mature stereoscopic imaging technique applied to the same scene from two independent platforms to unambiguously retrieve atmospheric motion vectors (AMVs) with accurate height assignment. This capability has been successfully applied to geostationary and low-earth orbit satellites to achieve excellent accuracy. When applied to a ground-point validation case, the accuracy for the CMIS aircraft observations was 20 m and 0.3 m/s for cloud heights and motion vectors, respectively. This result was confirmed by a detailed error analysis with analytical and covariance models. The results for CMIS cases with underflights of Aeolus, CALIPSO, and Aqua provided a good validation of expected accuracies. The paper also showed the feasibility of accommodating CMIS on CubeSats to enable multiple instruments to be flown in a leader–follower mode.
Unsupervised Optical Classification of the Seabed Color in Shallow Oligotrophic Waters from Sentinel-2 Images: A Case Study in the Voh-Koné-Pouembout Lagoon (New Caledonia)
Authors: Wattelez Guillaume , Dupouy Cécile , Juillot Farid
Published: February 2022

Monitoring chlorophyll-a concentration or turbidity is crucial for understanding and managing oligo- to mesotrophic coastal waters quality. However, mapping bio-optical components from space in such shallow settings remains challenging because of the strong interference of the complex bathymetry and various seabed colors. Correcting the total satellite reflectance signal from the seabed reflectance in ocean color with high resolution sensors is promising. This article shows how unsupervised clustering approaches can be applied to Sentinel-2 images to classify seabed colors in shallow waters of a tropical oligotrophic lagoon in New Caledonia. Data processing included Lyzenga correction for estimating the water column reflectance, optical spectra standardization for attenuating water absorption effects and clustering using the unsupervised k-means method. This methodological approach was applied on the 497, 560, 664 and 704 nm optical bands of the selected Sentinel-2 image. When applied on non-standardized data, our unsupervised classification retrieved three seafloor clusters, whereas five seafloor clusters could be retrieved using standardized data. For each of these two trials, the computed membership values explained more than 75% of the inertia in each Sentinel-2 wavelength band used for the clustering. However, the accuracy of the method was slightly improved when applied on standardized data. Confusion index mapping of the unsupervised clustering retrieved from these data emphasized the relevance and robustness of our methodological approach. Such an approach for seabed colors classification in optically complex shallow settings will be particularly helpful to improve remote sensing of biogeochemical indicators such as chlorophyll-a concentration and turbidity in fragile coastal environments.
Assessment of the Effectiveness of Sand-Control and Desertification in the Mu Us Desert, China
Authors: Li Jie , Wu Weicheng , Fu Xiao , Jiang Jingheng , Liu Yixuan , Zhang Ming , Zhou Xiaoting , Ke Xinxin , He Yecheng , Li Wenjing , Zhou Cuimin , Li Yuan , Song Yifei , Yang Hongli , Tu Qihong
Published: February 2022

The first successful sand-control was achieved in the Mu Us Desert by local people in the 1950–1960s, and their experience and approach have been extended to the whole Ordos and Northern China since then. The objective of this paper is to assess comprehensively the effectiveness of sand-control in 15 counties in and around Mu Us using multitemporal satellite images and socioeconomic data. After atmospheric correction, Landsat TM and OLI images were harnessed for land cover classification based on the ground-truth data and for derivation of the GDVI (generalized difference vegetation index) to extract the biophysical changes of the managed desert and desertification. Climatic, socioeconomic, environmental and spatial factors were selected for coupling analysis by multiple linear and logistic regression models to reveal the driving forces of desertification and their spatial determinants. The results show that from 1991 to 2020, 8712 km2 or 63% of the desert has been converted into pastures and shrublands with a greenness increase of 0.3509 in GDVI; the effectiveness of sand-control is favored by the rational agropastoral activities and policies; though desertification occurs locally, it is associated with both climatic and socioeconomic factors, such as wind speed, precipitation, water availability, distance to roads and animal husbandry.
InSAR Modeling and Deformation Prediction for Salt Solution Mining Using a Novel CT-PIM Function
Authors: Xing Xuemin , Zhang Tengfei , Chen Lifu , Yang Zefa , Liu Xiangbin , Peng Wei , Yuan Zhihui
Published: February 2022

Deformation prediction for a salt solution mining area is essential to mining environmental protection. The combination of Synthetic Aperture Radar Interferometry (InSAR) technique with Probability Integral Method (PIM) has proven to be powerful in predicting mining-induced subsidence. However, traditional mathematical empirical models (such as linear model or linear model combined with periodical function) are mostly used in InSAR approaches, ignoring the underground mining mechanisms, which may limit the accuracy of the retrieved deformations. Inaccurate InSAR deformations will transmit an unavoidable error to the estimated PIM parameters and the forward predicted subsidence, which may induce more significant errors. Besides, theoretical contradictory and non-consistency between InSAR deformation model and future prediction model is another limitation. This paper introduces the Coordinate-Time (CT) function into InSAR deformation modeling. A novel time-series InSAR model (namely, CT-PIM) is proposed as a substitute for traditional InSAR mathematical empirical models and directly applied for future dynamic prediction. The unknown CT-PIM parameters can be estimated directly via InSAR phase observations, which can avoid the error propagation from the InSAR-generated deformations. The new approach has been tested by both simulated and real data experiments over a salt mine in China. The root mean square error (RMSE) is determined as ±10.9 mm, with an improvement of 37.2% compared to traditional static PIM prediction method. The new approach provides a more robust tool for the forecasting of mining-induced hazards in salt solution mining areas, as well as a reference for ensuring the environment protection and safety management.
Effect of Time Window on Satellite and Ground-Based Data for Estimating Chlorophyll-a in Reservoirs
Authors: Kayastha Priya , Dzialowski Andrew R. , Stoodley Scott H. , Wagner Kevin L. , Mansaray Abubakarr S.
Published: February 2022

Algal blooms in freshwater ecosystems can negatively impact aquatic and human health. Satellite remote sensing of chlorophyll a (Chl-a) is often used to help determine the severity of algal blooms. However, satellite revisit flyover schedules may not match the erratic nature of algal blooms. Studies have paired satellite and ground-based data that were not collected on the same day, assuming Chl-a concentrations did not change significantly by the flyover date. We determined the effects of an increasing time window between satellite overpass dates and field-based collection of Chl-a on algorithms for Landsat 5, Landsat 8, and Sentinel-2, using 14 years (2006–2020) of Chl-a data from 10 Oklahoma reservoirs. Multiple regression models were built, and selected statistics were used to rank the time windows. The Sentinel-2 results showed strong relationships between Chl-a and satellite data collected up to a ±5-day window. The strength of these relationships decreased beyond a ±3-day time window for Landsat 8 and a ±1-day time window for Landsat 5. Our results suggest that the time window between field sampling and satellite overpass can impact the use of satellite data for Chl-a monitoring in reservoirs. Furthermore, longer time windows can be used with higher resolution (spatial, spectral) satellites.