bluuebunny/crossref_metadata_2025 huggingface dataset:
Breastfeeding Practices and Food Consumption of Socially Vulnerable Children
Authors: Oliveira Natália A. , Pizato Nathalia , Patriota Érika S. O. , do Carmo Ariene S. , Buccini Gabriela , Gonçalves Vivian S. S.
Published: January 2025

Promoting child well-being and development requires a multidimensional approach, including the right to adequate food practices. Socially vulnerable children are more exposed to adverse experiences, such as inadequate food consumption due to poverty. In this context, home-visiting programs are an important strategy for nutritional and health care education to provide relevant guidelines. This study describes breastfeeding and food consumption of children aged 0 to 24 months assisted by the Happy Child Program (Programa Criança Feliz—PCF) and aimed to investigate their association with socioeconomic factors and adherence to the program. This is an observational study, with a cross-sectional design, carried out with children assisted by the PCF in the Federal District, Brazil. Multivariate analysis was performed to identify sociodemographic and income factors, and household visits characteristics associated with breastfeeding, dietary diversity, and the consumption of ultra-processed food. A total of 301 children were assessed, 51.16% of whom were female. In 58.99% of households, the reference person was the mother; 86.20% were unemployed, and 27.08% had a low education degree. About 62.65% of beneficiaries lived on up to USD 200.00 per month and the majority faced food insecurity. The child’s age, and the mother’s current work situation were independently associated with the current breastfeeding situation, especially higher among women who did not work (p = 0.015). The minimum dietary diversity among children over 6 months old was 62.21% and presented a positive association with adherence to the program (p = 0.005). On the other hand, the consumption of ultra-processed foods was 77.21% and was associated with a longer follow-up time within the program (p = 0.047). The associations observed revealed the need to integrate family food choices and nutritional education into public policies for early childhood.
Metabolomics Unveiled the Accumulation Characteristics of Taste Compounds During the Development and Maturation of Litchi Fruit
Authors: Jiang Nonghui , Liu Wei , Xiao Zhidan , Xiang Xu , Zhong Yun
Published: January 2025

Litchi is one of the ancient fruits that originated in China, renowned for its high nutrition and rich flavor, and Xianjinfeng (XJF) stands as one of the most notable varieties in terms of its flavor. Investigating the metabolic changes in taste compounds during fruit development offers deeper insights into the formation patterns of fruit quality. In this study, we conducted extensive metabonomic research on the accumulation patterns of taste compounds (carbohydrates, organic acids, and amino acids) across three developmental stages of XJF litchi. A total of 238 taste metabolites were detected. Cluster analysis and PCA revealed significant changes in metabolite composition and content across different stages, closely correlating with the developmental phase. The abundance of total taste metabolites in stage S1 was notably lower than stages S2 and S3. The total abundance of sugar continued to rise, yet monosaccharides and disaccharides exhibited distinct behaviors, highlighting the characteristic accumulation of reducing sugars. Most organic acids demonstrated a notable downward trend, whereas the abundance of most essential and flavor-contributing amino acids showed an upward trend. The number of DAMs across the three stages followed the trend of S1 vs. S3 > S1 vs. S2 > S2 vs. S3. KEGG functional annotation and enrichment revealed that amino acid biosynthesis, D-amino acid metabolism, 2-oxocarboxylic acid metabolism, glyoxylate and dicarboxylate metabolism, the pentose phosphate pathway, the tricarboxylic acid cycle, and carbon metabolism were the most significantly enriched primary metabolic pathways. More differential metabolites and metabolic pathways indicated that the critical stage from the green fruit stage to the color transition stage laid a solid foundation for litchi flavor. This experiment will offer valuable references for cultivation, breeding, processing, and consumption.
Pharmacogenetics of the Treatment of Neglected Diseases
Authors: Cabral Tiffany Borges , de Oliveira Amanda Carvalho , Melo Gisely Cardoso de , Rodrigues-Soares Fernanda
Published: January 2025

Background/Objectives: Pharmacogenetics (PGx) aims to identify individuals more likely to suffer from adverse reactions or therapeutic failure in drug treatments. However, despite most of the evidence in this area being from European populations, some diseases have also been neglected, such as HIV infection, malaria, and tuberculosis. With this review, we aim to emphasize which pharmacogenetic tests are ready to be implemented in treating neglected diseases that have some evidence and call attention to what is missing for these three diseases. Methods: A critical literature review on the PGx of HIV infection, malaria, and tuberculosis was performed. Results: There are three PGx guidelines for antiretroviral drugs used in HIV infection, one for malaria, and none for tuberculosis. Some evidence is already available, and some genes have already been identified, such as CYP2D6 for primaquine treatment and NAT2 for isoniazid. However, some barriers to the implementation are the lack of evidence due to the few studies on the diseases themselves and the admixture of the most affected populations, which must be considered, given the genetic differentiation of these populations. Conclusions: PGx tests such as abacavir are already implemented in some places, and efavirenz/atazanavir is ready to implement if this medication is used. Other gene–drug associations were found but still do not present a clear recommendation. We call attention to the need to generate more evidence for testing treatments for other neglected diseases, such as malaria and tuberculosis, given their epidemiological importance and for the public health of less favored populations.
Hydrometeorological Variability of Olive Ridley Sea Turtle (Lepidochelys olivacea) Nesting Beaches: Implications for Conservation Practices
Authors: Filonov Anatoliy , Godínez-Domínguez Enrique , Tereshchenko Iryna , Monzon Cesar O. , Avalos-Cueva David , Barba-López María del Refugio
Published: January 2025

The conservation of the olive ridley turtle (Lepidochelys olivacea) is increasingly critical due to declining global populations. This study investigates the influence of hydrometeorological conditions on the nesting season and annual hatchling sex ratios conducted at the Playón de Mismaloya Federal Reserve in Tomatlán, Jalisco, Mexico. The research specifically examines variations in sand temperature at both the beach surface and nesting depths, with extended measurements taken at multiple depths (20, 40, 60, 80, and 100 cm) to analyze the vertical temperature gradient along the beach. Atmospheric parameters were modeled using Newton’s Cooling Law and solved with the finite difference method to estimate heat loss rates from beach sand to its surroundings, shedding light on microclimatic effects on incubation and embryonic development. Meteorological data were gathered from an automatic weather station, while sand temperatures were monitored with thermographs. During the warm period (approximately 32 °C), sand temperature showed a negative correlation with depth (20–100 cm), indicating cooler temperatures at greater depths. These conditions were associated with female-biased hatchling production. Conversely, the cold period (approximately 28 °C) led to male-biased hatchling production, with a positive correlation between sand and air temperatures. This study emphasizes the importance of monitoring in situ environmental conditions and extending the protection season until February to avoid the loss of male hatchlings.
Detection and Attribution of a Spatial Heterogeneity in the Temporal Evolution of Bulgarian River Discharge
Authors: Kilifarska Natalya A. , Metodieva Gergana I. , Mokreva Antonia Ch.
Published: January 2025

The hydrosphere is an element of the climate system and changes in the latter are reasonably projected over the river outflow. Climatic changes, however, are unevenly distributed over the Earth, and understanding their regional imprint on the hydrosphere is of great importance. In this study, we have conducted a statistical analysis of the monthly maximum and minimum river discharge recorded in 22 hydrological stations located on 19 of the Bulgarian rivers during the period 1993–2022. We have found that in half of the river basins, the trend of the spring maximum discharge is significantly positive (α = 0.05). In the other half of the stations, the trend is neutral. The stations with a positive trend are not randomly distributed but grouped, forming a pattern crossing the country from northwest to southeast. This pattern of trend distribution raises questions about the causes of the irregular hydrological response to the rising global near-surface temperatures. A comparison of hydrological data with some climatic variables (i.e., temperature, precipitation, and ozone at 70 hPa), combined with neural network analysis results, suggests ozone as a possible reason for the heterogeneous hydrological response. Its effect could be explained by an imposed episodic warming of the near-surface temperature due to fluctuations in the ozone density near the tropopause, which in turn favours the faster melting of ice and snow in the corresponding river basins.
Predicting Hospitalization in Older Adults Using Machine Learning
Authors: Buenrostro-Mariscal Raymundo , Montesinos-López Osval A. , Gonzalez-Gonzalez Cesar
Published: January 2025

Background/Objectives: Hospitalization among older adults is a growing challenge in Mexico due to the high prevalence of chronic diseases and limited public healthcare resources. This study aims to develop a predictive model for hospitalization using longitudinal data from the Mexican Health and Aging Study (MHAS) using the random forest (RF) algorithm. Methods: An RF-based machine learning model was designed and evaluated under different data partition strategies (ST) with and without variable interaction. Variable importance was assessed based on the mean decrease in impurity and permutation importance, enhancing our understanding of predictors of hospitalization. The model’s robustness was ensured through modified nested cross-validation, with evaluation metrics including sensitivity, specificity, and the kappa coefficient. Results: The model with ST2, incorporating interaction and a 20% test proportion, achieved the best balance between sensitivity (0.7215, standard error ± 0.0038), and specificity (0.4935, standard error ± 0.0039). Variable importance analysis revealed that functional limitations (e.g., abvd3, 31.1% importance), age (12.75%), and history of cerebrovascular accidents (12.4%) were the strongest predictors. Socioeconomic factors, including education level (12.08%), also emerged as critical predictors, highlighting the model’s ability to capture complex interactions between health and socioeconomic variables. Conclusions: The integration of variable importance analysis enhances the interpretability of the RF model, providing novel insights into the predictors of hospitalization in older adults. These findings underscore the potential for clinical applications, including anticipating hospital demand and optimizing resource allocation. Future research will focus on integrating subgroup analyses for comorbidities and advanced techniques for handling missing data to further improve predictive accuracy.
Predictive Factors of Resilience in Early Childhood Care Professionals
Authors: Gómez-Herrera Sofía , Robles-Bello Maria Auxiliadora , Sánchez-Teruel David , Sarhani-Robles Aziz
Published: January 2025

Background/Objectives: Early childhood intervention professionals have higher rates of work-related stress and burnout compared to other health professionals. Furthermore, this is exacerbated by exposure to negative emotions, the stigma associated with mental health, and even the stress experienced by families due to the impact of having a child with a developmental disability. The aim of this study was to determine whether emotional intelligence and empathy were able to predict resilience in early childhood care professionals. Methods: The total sample consisted of 139 people (128 women and 11 men, with a mean age of 32.69 and SD 9.72) who were divided into two groups: high resilience (M = 35.85; SD = 3.64) and low resilience (M = 20.74; SD = 3.84). Results: The results showed significant differences between the two groups in self and others’ emotional appraisal, use and regulation of emotion, perspective taking, and personal distress, with a positive relationship between resilience and all sub-dimensions of emotional intelligence and perspective taking and a negative relationship with personal distress. In addition, a predictive model of resilience in early childhood professionals was found with empathic concern, personal distress, and use of emotion. Conclusions: This study is useful to start investigating psychological aspects related to early intervention and its professionals in order to consolidate a resilient workforce.
Healthcare Application of Failure Mode and Effect Analysis (FMEA): Is There Room in the Infectious Disease Setting? A Scoping Review
Authors: Vecchia Marco , Sacchi Paolo , Marvulli Lea Nadia , Ragazzoni Luca , Muzzi Alba , Polo Lorenzo , Bruno Raffaele , Salio Flavio
Published: January 2025

Background: Failure mode and effect analysis (FMEA) is a valuable risk analysis tool aimed at predicting the potential failures of a system and preventing them from occurring. Since its initial use, it has also recently been applied to the healthcare setting, which has been made progressively more complex by technological developments and new challenges. Infection prevention and control (IPC) is an area that requires effective strategies. The aim of this study is to review the literature on the employment of FMEA in the healthcare environment, with special consideration for its application in the infectious disease setting. Methods: An extensive search was carried out in two international and public databases, PUBMED and EMBASE; we included all studies regarding the use of FMEA in hospital settings and human patient care processes. Results: A total of 163 studies published over the period from 2003 to 2023 were included for data extraction. These studies were analyzed regarding bibliometric data (publication year and country of origin), the healthcare issues to be addressed, the application fields, and the utilized FMEA methods. Among these, 13 studies were found that took an interest in infectious diseases. Conclusions: FMEA can be effectively used for healthcare risk assessment. Its implementation as a standard tool in healthcare settings, though demanding, may serve as an important tool for preventing the risk of biohazard incidents, epidemics, and environmental contamination, thereby improving safety for both patients and healthcare workers.
The Effects of Social Feedback Through the “Like” Feature on Brain Activity: A Systematic Review
Authors: Dores Artemisa R. , Peixoto Miguel , Fernandes Carina , Marques António , Barbosa Fernando
Published: January 2025

Background: Problematic social media (SM) use is a growing concern, particularly among adolescents who are drawn to these platforms for social interactions important to their age group. SM dependence is characterized by excessive, uncontrolled usage that impairs personal, social, and professional aspects. Despite the ongoing debate over recognizing SM addiction as a distinct diagnostic category, the impact of social feedback, particularly through the “like” button, on brain activity remains under scrutiny. Objective: This systematic review aims to study the neural correlates of online social feedback, focusing on the effects of the “like” feedback on brain activity using fMRI and EEG. Methods: The review followed the recommendations of the Preferred Reporting Items for Systematic Reviews and Meta-Analysis Protocols (PRISMA). Results: The review included 11 studies with 504 participants, identifying key brain structures such as the amygdala, ventromedial prefrontal cortex (vmPFC), and ventral striatum involved in reward processing. Positive feedback (“likes”) activates areas like the nucleus accumbens (NACC), vmPFC, and amygdala, with NACC correlating with increased SM use intensity. Negative feedback activates the ventrolateral prefrontal cortex (vlPFC) and left medial prefrontal cortex (mPFC). Behavioral data indicates that positive feedback influences subsequent social interactions. Conclusions: The review highlights disparities in the literature regarding the neural response to social feedback, emphasizing the need for further research to clarify the roles of sex, personality traits, and the person giving feedback. Overall, understanding the neurobiological underpinnings of SM engagement is essential for developing effective interventions to prevent or address the negative effects of excessive SM use.
Exploring New Tools in Upper Limb Rehabilitation After Stroke Using an Exoskeletal Aid: A Pilot Randomized Control Study
Authors: Syringas Pantelis , Potsika Vassiliki , Tachos Nikolaos , Pardalis Athanasios , Papaioannou Christoforos , Mitsis Alexandros , Pakos Emilios E. , Zestas Orestis N. , Papagiannis Georgios , Triantafyllou Athanasios , Tselikas Nikolaos D. , Yiannopoulou Konstantina G. , Papathanasiou George , Georgoudis George , Bakalidou Daphne , Kyriakidou Maria , Gkrilias Panagiotis , Kakkos Ioannis , Matsopoulos George K. , Fotiadis Dimitrios I.
Published: January 2025

Background/Objectives: Spasticity commonly occurs in individuals after experiencing a stroke, impairing their hand function and limiting activities of daily living (ADLs). In this paper, we introduce an exoskeletal aid, combined with a set of augmented reality (AR) games consisting of the Rehabotics rehabilitation solution, designed for individuals with upper limb spasticity following stroke. Methods: Our study, involving 60 post-stroke patients (mean ± SD age: 70.97  ±  4.89 years), demonstrates significant improvements in Ashworth Scale (AS) scores and Box and Block test (BBT) scores when the Rehabotics solution is employed. Results: The intervention group showed slightly greater improvement compared to the control group in terms of the AS (−0.23, with a confidence interval of −0.53 to 0.07) and BBT (1.67, with a confidence interval of 1.18 to 2.16). Additionally, the Rehabotics solution was particularly effective for patients with more severe deficits. Patients with an AS score of 3 showed more substantial improvements, with their AS scores increasing by −1.17 ± 0.39 and BBT scores increasing by −4.83 ± 0.72. Conclusions: These findings underscore the potential of wearable hand robotics in enhancing stroke survivors’ hand rehabilitation, emphasizing the need for further investigations into its broader applications.
Bacillus thuringiensis and Trichoderma asperellum as Biostimulants in Hydroponic Tendril Pea (Pisum sativum) Microgreens
Authors: Pérez-Leal Ramona , Rodríguez-Roque María Janeth , Acevedo-Barrera Angélica Anahí , Villa-Martínez Alejandra , Guerrero Brenda I. , Gutiérrez-Chávez Aldo , Hernández-Huerta Jared
Published: January 2025

The study evaluated the effect of Bacillus thuringiensis (Bt) and Trichoderma asperellum (Ta) as biostimulants in hydroponically grown tendril pea (Pisum sativum) microgreens. A completely randomized experimental design was conducted under controlled conditions; the study included the root pea-spraying treatment with Bt, Ta, and their combination, alongside a non-inoculated control. The results showed that treatments with Ta significantly enhanced growth parameters, such as stem length and diameter, stipular leaf area, and fresh weight of the microgreens. Specifically, fresh biomass increased by 140% and dry biomass by 80% with Ta compared to the control, while combined treatment with Bt and Ta reduced nitrates by 39.6%. Bt and Bt + Ta increased chlorophyll b and carotenoids contents, suggesting improved photosynthetic activity. No significant differences in visual appearance were observed. In conclusion, the application of Ta and Bt can enhance tendril pea microgreens’ yield and certain biochemical (chlorophyll b and carotenoids) qualities without affecting their visual quality, supporting the application of these microorganisms as biostimulants. These findings underscore the potential to scale these treatments in commercial hydroponic systems, offering a sustainable approach to optimizing the production of this emerging crop type.
Genome-Wide Identification of the CIPK Gene Family in Jasmine and Expression Analysis Under Salt Stress
Authors: Zhang Shuang , Huang Xin , Yin Lili , Li Jiawei , Xu Jiacan , Wu Ruigang
Published: January 2025

Various CBL-interacting protein kinases (CIPKs) are involved in abiotic stress responses in plants. Despite the economic importance of jasmine (Jasminum sambac L. Aiton) and the availability of genome data, there are few reports analyzing the CIPK gene family. In this study, genome-wide identification of the CIPK gene family in jasmine was conducted, which would provide valuable information for the function analysis of JsCIPKs regarding participation in growth and development and response to salt stress. In the present study, a total of 17 CIPKs were identified, which were unevenly distributed on eight chromosomes. The JsCIPK protein sequences contained 311–781 amino acids, with a molecular weight of 35.05–87.58 kDa. Phylogenetic analysis revealed that the 17 JsCIPKs could be divided into five classical branches. JsCIPK genes with higher homology showed greater similarity between conserved protein motifs. Collinearity analysis demonstrated that 13 gene pairs in Arabidopsis were collinear with the jasmine sequences. Various hormone-related response- and stress-induced elements were observed in the promoter region of JsCIPK genes, such as TC-rich repeats, CARE, etc. Furthermore, the expression of JsCIPK genes varied in different organs. Finally, the expression analyses of eight JsCIPKs under salt stress were performed. A systematic analysis of the CIPK gene family and the effect of salt stress on the expression of eight JsCIPK genes in leaves of jasmine was carried out. The expression of JsCIPK6 and JsCIPK8 was significantly down-regulated and up-regulated by salt treatment, respectively. These findings would lay a foundation for future functional studies of these two genes in jasmine related to salt stress and provide useful resistance genes for the molecular breeding of new varieties of salt-tolerant jasmine.
Genome-Wide Identification of miRNAs in Oily Persimmon (Diospyros oleifera Cheng) and Their Functional Targets Associated with Proanthocyanidin Metabolism
Authors: Zhang Meng , Wu Rong , Hu Xinlong , Luo Zhengrong , Zhang Qinglin , Yang Sichao
Published: January 2025

Cultivated persimmon (Diosspyros kaki Thunb.) is a hexaploid (mostly) or a nonaploid with high heterozygosity, hindering molecular genetic studies on proanthocyanidin (PA) metabolism, which is a major trait for persimmon astringency. Recently, one of its wild diploid relative species, oily persimmon (Diospyros oleifera), has been assembled with a chromosome-level reference. Thus, oily persimmon is now regarded as a model plant for discovering new genes associated with PA metabolism, which is highly accumulated in the fruits of this genus. In our study, we identified genome-wide microRNAs (miRNAs) and their precursor sequence based on the chromosome-scale genome of oily persimmon and the miRNA database of “Eshi 1” according to the sequence alignment and secondary structure accession. The targets were predicted on the psRNATarget software based on the genome CDS database. The size, conservation, diversity, stem-loop hairpin structures, and genome location of miRNA or the precursor sequence were analyzed by bioinformatics tools. The promoter elements of the miRNA genes were predicted on the promoter-2.0 software, which indicated that the abundant cis-acting elements were light responsiveness, promoter, and enhancer regions. The qRT-PCR assay was performed to elucidate the potential expression patterns of precursor miRNA and their targets during fruit development, and one target gene, DkMYB22, of miR2911 was verified to promote the conversion of soluble tannins into insoluble tannins involved in the deastringency in persimmons. Together, this study provides a robust foundation for further functional verification of these miRNAs associated with the natural deastringency process in persimmon, thereby facilitating advancements in persimmon fruit breeding.
MLAS: Machine Learning-Based Approach for Predicting Abiotic Stress-Responsive Genes in Chinese Cabbage
Authors: You Xiong , Shu Yiting , Ni Xingcheng , Lv Hengmin , Luo Jian , Tao Jianping , Bai Guanghui , Feng Shusu
Published: January 2025

The challenges posed by climate change have had a crucial impact on global food security, with crop yields negatively affected by abiotic and biotic stresses. Consequently, the identification of abiotic stress-responsive genes (SRGs) in crops is essential for augmenting their resilience. This study presents a computational model utilizing machine learning techniques to predict genes in Chinese cabbage that respond to four abiotic stresses: cold, heat, drought, and salt. To construct this model, data from relevant studies regarding responses to these abiotic stresses were compiled, and the protein sequences encoded by abiotic SRGs were converted into numerical representations for subsequent analysis. For the selected feature set, six distinct machine learning binary classification algorithms were employed. The results demonstrate that the constructed models can effectively predict SRGs associated with the four types of abiotic stresses, with the area under the receiver operating characteristic curve (auROC) for the models being 81.42%, 87.92%, 80.85%, and 88.87%, respectively. For each type of stress, a distinct number of stress-resistant genes was predicted, and the ten genes with the highest scores were selected for further analysis. To facilitate the implementation of the proposed strategy by users, an online prediction server, has been developed. This study provides new insights into computational approaches to the identification of abiotic SRGs in Chinese cabbage as well as in other plants.
The Molecular Biology of Placental Transport of Calcium to the Human Foetus
Authors: Walker Valerie
Published: January 2025

From fertilisation to delivery, calcium must be transported into and within the foetoplacental unit for intracellular signalling. This requires very rapid, precisely located Ca2+ transfers. In addition, from around the eighth week of gestation, increasing amounts of calcium must be routed directly from maternal blood to the foetus for bone mineralisation through a flow-through system, which does not impact the intracellular Ca2+ concentration. These different processes are mediated by numerous membrane-sited Ca2+ channels, transporters, and exchangers. Understanding the mechanisms is essential to direct interventions to optimise foetal development and postnatal bone health and to protect the mother and foetus from pre-eclampsia. Ethical issues limit the availability of human foetal tissue for study. Our insight into the processes of placental Ca2+ handling is advancing rapidly, enabled by developing genetic, analytical, and computer technology. Because of their diverse sources, the reports of new findings are scattered. This review aims to pull the data together and to highlight areas of uncertainty. Areas needing clarification include trafficking, membrane expression, and recycling of channels and transporters in the placental microvilli; placental metabolism of vitamin D in gestational diabetes and pre-eclampsia; and the vascular effects of increased endothelial Orai expression by pregnancy-specific beta-1-glycoproteins PSG1 and PSG9.
Alveolar Macrophages in Viral Respiratory Infections: Sentinels and Saboteurs of Lung Defense
Authors: Pöpperl Pauline , Stoff Melanie , Beineke Andreas
Published: January 2025

Respiratory viral infections continue to cause pandemic and epidemic outbreaks in humans and animals. Under steady-state conditions, alveolar macrophages (AlvMϕ) fulfill a multitude of tasks in order to maintain tissue homeostasis. Due to their anatomic localization within the deep lung, AlvMϕ are prone to detect and react to inhaled viruses and thus play a role in the early pathogenesis of several respiratory viral infections. Here, detection of viral pathogens causes diverse antiviral and proinflammatory reactions. This fact not only makes them promising research targets, but also suggests them as potential targets for therapeutic and prophylactic approaches. This review aims to give a comprehensive overview of the current knowledge about the role of AlvMϕ in respiratory viral infections of humans and animals.
CD8+ and CD8− NK Cells and Immune Checkpoint Networks in Peripheral Blood During Healthy Pregnancy
Authors: Meggyes Matyas , Nagy David U. , Mezosi Livia , Polgar Beata , Szereday Laszlo
Published: January 2025

Pregnancy involves significant immunological changes to support fetal development while protecting the mother from infections. A growing body of evidence supports the importance of immune checkpoint pathways, especially at the maternal–fetal interface, although limited information is available about the peripheral expression of these molecules by CD8+ and CD8− NK cell subsets during the trimesters of pregnancy. Understanding the dynamics of these immune cells and their checkpoint pathways is crucial for elucidating their roles in pregnancy maintenance and potential complications. This study aims to investigate the peripheral expression and functional characteristics of CD8+ and CD8− NK cell subsets throughout pregnancy, providing insights into their contributions to maternal and fetal health. A total of 34 healthy women were enrolled from the first, 30 from the second and 40 from the third trimester of pregnancy. At the same time, 35 healthy age-matched non-pregnant women formed the control group. From peripheral blood, mononuclear cells were separated and stored at −80 °C. CD8+ and CD8− NK cell subsets were analyzed from freshly thawed samples, and surface and intracellular staining was performed using flow cytometric analyses. The proportions of CD56+ NK cells in peripheral blood were similar across groups. While CD8− NKdim cells increased significantly in all trimesters compared to non-pregnant controls, CD8+ NKdim cells showed no significant changes. CD8− NKbright cells had higher frequencies throughout pregnancy, whereas CD8+ NKbright cells significantly increased only in the first and second trimesters. The expression levels of immune checkpoint molecules, such as PD-1 and PD-L1, and cytotoxic-activity-related molecules were stable, with notable perforin and granzyme B increases in CD8− NKbright cells throughout pregnancy. Our study shows that peripheral NK cell populations, especially CD8− subsets, are predominant during pregnancy. This shift suggests a crucial role for CD8− NK cells in balancing maternal immune tolerance and surveillance. The stable expression of immune checkpoint molecules indicates that other regulatory mechanisms may be at work. These findings enhance our understanding of peripheral immune dynamics in pregnancy and suggest that targeting CD8− NKbright cell functions could help manage pregnancy-related immune complications. This research elucidates the stable distribution and functional characteristics of peripheral NK cells during pregnancy, with CD8− subsets being more prevalent. The increased activity of CD8− NKbright cells suggests their critical role in maintaining immune surveillance. Our findings provide a basis for future studies to uncover the mechanisms regulating NK cell function in pregnancy, potentially leading to new treatments for immune-related pregnancy complications.
Comprehensive In Silico Analysis of the NHX (Na+/H+ Antiporter) Gene in Rice (Oryza sativa L.)
Authors: Bui Hoa Hai Thi , Nguyen Duong Huy , Dinh Le Thu Thi , Trinh Hang Thu Thi , Vu Thoa Kim , Bui Van Ngoc
Published: January 2025

The Na+/H+ antiporter (NHX) gene family plays a pivotal role in plant salt tolerance in regulating intracellular Na+ and H+ homeostasis. In this study, seven candidate OsNHX genes (OsNHX1 to OsNHX7) were identified in the rice genome and classified into three phylogenetic clusters (Vac, Endo, and PM) based on their predicted subcellular localization. Five OsNHX gene pairs (OsNHX1/OsNHX2, OsNHX1/OsNHX3, OsNHX1/OsNHX4, OsNHX2/OsNHX6, and OsNHX5/OsNHX6) were found to have arisen from dispersed duplication events and exhibited purifying selection, indicating functional conservation. Analysis of cis-regulatory elements (CREs) revealed a diverse range of elements associated with tissue-specific expression, hormone signaling, and stress responses, particularly to dehydration and salinity. Notably, CREs associated with tissue/organelle-specific expression and stress responses were the most abundant, suggesting a potential role for OsNHX genes in regulating growth, development, and stress tolerance in rice. Importantly, expression profiling revealed that OsNHX1, OsNHX2, OsNHX3, and OsNHX5 were upregulated under salt stress, with significantly higher expression levels in the salt-tolerant rice cultivar Pokkali compared to the salt-sensitive cultivar IR64. Our findings provide a comprehensive analysis of the evolutionary, structural, and functional features of the OsNHX gene family and highlights their critical role in rice salt tolerance, offering insights into potential applications for crop improvement.
Relay Node Selection Methods for UAV Navigation Route Constructions in Wireless Multi-Hop Network Using Smart Meter Devices
Authors: Ohkawa Shuto , Ueda Kiyoshi , Miyoshi Takumi , Yamazaki Taku , Yamamoto Ryo , Funabiki Nobuo
Published: January 2025

Unmanned aerial vehicles (UAVs) offer solutions to issues like traffic congestion and labor shortages. We developed a distributed UAV management system inspired by virtual circuit and datagram methods in packet-switching networks. By installing houses with wireless terminals, UAVs navigate routes in a multi-hop network, communicating with ground nodes. UAVs are treated as network packets, ground devices are treated as routers, and their connections are treated as links. Activating all nodes as relays increases control message traffic and node load. To optimize connectivity, we minimize relay nodes, connecting non-relay nodes to the nearest relay. This study proposes four relay node selection methods: random selection, two adjacency-based methods, and our innovative approach using Multipoint Relay (MPR) from the Optimized Link State Routing Protocol (OLSR). We evaluated these methods according to their route construction success rates, relay node counts, route lengths, and so on. The MPR-based method proved most effective for UAV route construction. However, fewer relay nodes increase link collisions, and we identify the minimum relay density needed to balance efficiency and conflict reduction.
Analyzing Transit Systems Using General Transit Feed Specification (GTFS) by Generating Spatiotemporal Transit Networks
Authors: Liu Diyi , Guo Jing , Gu Yangsong , King Meredith , Han Lee D. , Brakewood Candace
Published: January 2025

The General Transit Feed Specification (GTFS) is an open standard format for recording transit information, utilized by thousands of transit agencies worldwide. In this study, a new tool named GTFS2STN for converting GTFS data into the spatiotemporal networks is introduced. To analyze the travel time variability, it is important to transform a transit network to a spatiotemporal network to enable a comprehensive analysis of transit system accessibility. GTFS2STN is a new tool that converts General Transit Feed Specification (GTFS) data into spatiotemporal networks, addressing the lack of open-source solutions for transit analysis. The tool includes a web application that generates isochrone maps and calculates travel time variability between locations. Validation against Google Maps APIs shows that journey time (i.e., the summation of the transit time, walking time, and waiting time) differences in the Mean Absolute Percentage Error are typically within 12%. A before–after analysis shows that for the transit journey time in 2024 in Nashville, Tennessee, 8 out of 10 pivotal bus stops showed a significantly decreased journey time compared with the case of 2019. A further set of before–after analyses shows that although journey time between transit sites significantly dropped on May 2020 during COVID-19 emergencies, the journey time almost totally recovered to the before-COVID-19 level by November 2020. By supporting any valid GTFS schedule, GTFS2STN enables the analysis of historical and planned transit systems, making it valuable for long-term accessibility assessment and travel time variability studies.
Novel Machine Learning-Based Brain Attention Detection Systems
Authors: Wang Junbo , Kim Song-Kyoo
Published: January 2025

Electroencephalography (EEG) can reflect changes in brain activity under different states. The electrical signals of the brain are observed to exhibit varying amplitudes and frequencies. These variations are closely linked to different states of consciousness, influencing the internal and external behaviors, emotions, and learning performance of humans. The assessment of personal level of attention, which refers to the ability to consciously focus on something, can also be facilitated by these signals. Research on brain attention aids in the understanding of the mechanisms underlying human cognition and behavior. Based on the characteristics of EEG signals, this research identifies the most effective method for detecting brain attention by adapting various preprocessing and machine learning techniques. The results of our analysis on a publicly available dataset indicate that KNN with the feature importance feature extraction method performed the best, achieving 99.56% accuracy, 99.67% recall, and 99.44% precision with a rapid training time.
Detection of Anaplasma phagocytophilum DNA in Deer Keds: Massachusetts, USA
Authors: Pearson Patrick , Xu Guang , Siegel Eric L. , Ryan Mileena , Rich Connor , Feehan Martin J. R. , Dinius Blake , McAuliffe Shaun M. , Roden-Reynolds Patrick , Rich Stephen M.
Published: January 2025

Deer keds (Lipoptena spp. and Neolipoptena ferrisi) are hematophagous ectoparasites that primarily infest white-tailed deer (Odocoileus virginianus) and other cervids in the United States. The distribution of deer keds in the northeastern United States and the pathogens they harbor remains relatively unexplored. In this study, we examined the geographical distribution and pathogen prevalence of deer keds in Massachusetts by collecting samples from white-tailed deer and testing for tick-borne pathogens. Deer keds were collected across the state, including in four previously unrecorded counties, indicating a wide distribution. Pathogen screening revealed the presence of Anaplasma phagocytophilum DNA in 30% of the keds, but no other pathogens were detected. The medical and biological significance of detecting A. phagocytophilum DNA in deer keds requires future studies. This research provides a baseline for the distribution and pathogen prevalence of deer keds in Massachusetts and highlights the potential of deer keds as sentinels for monitoring deer-associated microbes.
Design, Development, and Testing of a New Device to Prevent High-Frequency Noise-Induced Damage: The “Dynamic Earplug”
Authors: Giorgi Lucrezia , Moffa Antonio , Mattaroccia Marco , Lopez Michele Antonio , Schena Emiliano , Casale Manuele
Published: January 2025

Hearing loss resulting from prolonged exposure to loud noise is known as noise-induced hearing loss (NIHL), and it often affects professionals exposed to occupational sources of high sound levels. Among the professionals chronically exposed to noise, dentists use instrumentation that produces high-frequency noise. In this occupational category, NIHL is estimated to reach a 5% to 20% prevalence of workers. However, dentists and healthcare personnel have no suitable personal protection equipment designed for their needs. The study aims to develop a new individual hearing protection device called the “dynamic earplug”, which protects from high-frequency noise and amplifies speech frequencies. Testing with the Fonix 7000 Hearing Aid Test System showed effective filtering of high frequencies (above 4000 Hz) from dental instruments and a speech frequency amplification of up to 13 dB (500 Hz–1000 Hz). In a trial involving 20 subjects during an 8 h work shift, most participants positively evaluated the device’s esthetics, ease of insertion, comfort, stability, and noise attenuation while still being able to hear patients’ and colleagues’ voices. The dynamic earplug shows promise as an efficient and comfortable hearing protection solution for professionals exposed to high-frequency noise.
The Impact of Diabetes Mellitus and Metformin Use on Outcomes After Endovascular Aneurysm Repair
Authors: van Merrienboer Tara A. R. , Warlich Veerle , Holewijn Suzanne , Driessen Wouter , Yeung Kak K. , Reijnen Michel M. P. J.
Published: January 2025

Objective: To study the influence of diabetes mellitus (DM) and metformin treatment on aneurysm sac remodeling after endovascular aneurysm repair (EVAR). Methods: A retrospective single-center cohort analysis was conducted on consecutive patients who underwent elective EVAR for an infrarenal abdominal aortic aneurysm (AAA) between January 2011 and December 2021. Differences between study groups were analyzed and Kaplan–Meier analysis were employed to describe overall and reintervention-free survival. Cox regression analysis was performed to identify predictors of sac shrinkage. Results: A total of 529 patients were included: 74 (14.0%) had DM and metformin treatment, 26 (4.9%) had DM without metformin treatment, and 429 (81.1%) did not have DM. At one-year follow-up, diabetic patients showed significantly less sac shrinkage compared to non-diabetic patients (40.0% vs. 52.0%; p = 0.038), with a trend toward more stable sac behavior in diabetic patients (52% vs. 42%; p = 0.055). At last follow-up, sac shrinkage was significantly less in diabetic patients on metformin treatment compared to non-diabetics (48.6% vs. 59.9%; p = 0.047). No differences in sac shrinkage were observed between diabetics with and without metformin treatment. The presence of endoleak was significantly higher in groups showing stable sac behavior and growth. Through nine-year follow-up, overall survival was significantly less in diabetic patients compared to non-diabetic ones (23.5% vs. 37.5%; p < 0.001). Conclusions: This study showed a negative impact of diabetes mellitus and metformin treatment on sac shrinkage following EVAR. The presence of any type of endoleak was associated with reduced sac shrinkage at both time points. Overall survival was significantly lower in diabetic patients compared to non-diabetic patients.
Relationship of Chronic Stress and Hypertension with Bone Resorption
Authors: Paulini Marina Ribeiro , Aimone Mariangeles , Feldman Sara , Buchaim Daniela Vieira , Buchaim Rogerio Leone , Issa João Paulo Mardegan
Published: January 2025

Background/Objectives: Chronic exposure to stress has been considered a risk factor for hypertension, which is also associated with increased bone resorption. This review aimed to investigate the effect of acute and chronic stress, associated with hypertension, on the skeletal system. Methods: A comprehensive search was conducted across multiple databases, focusing on peer-reviewed articles published in English. We include experimental, clinical, and peer-reviewed studies focused on the relationship between stress, hypertension, and bone resorption. Searches were conducted in MEDLINE via PubMed, Embase and Scopus, with the last search completed on 10 September 2024. Results: The main topics include situations that favor bone loss, such as psychological stress, which can lead to osteoporotic fractures through immunological and endocrine mechanisms. The relationship between psychological stress and loss of bone density, as in osteoporosis, occurs due to the reduction in the number of osteoblasts and loss in the balance between physiological formation/resorption. Conclusions: Chronic stress significantly affects cardiovascular health and bone resorption. This narrative review study highlights the vulnerability of the skeletal system, along with the cardiovascular system, to prolonged stress, emphasizing the need for multidisciplinary strategies in preventing stress-related conditions. Effective stress management can help reduce the risks of cardiovascular disease and bone resorption, emphasizing their role in comprehensive health care.
Evaluation of Optimized Lumbar Oblique X-Ray Angles with Positioning Assistance for Enhanced Imaging Quality: A Pilot Study in an Asian Cohort
Authors: Wang Yu-Li , Su Hsin-Yueeh , Cheng Chao-Min , Lee Kuei-Chen
Published: January 2025

Objective: Pars fractures are a common cause of lower back pain, especially among young individuals. Although computed tomography (CT) and magnetic resonance imaging (MRI) scanning are commonly used in developed regions, traditional radiography remains the main diagnostic method in many developing countries. This study assessed whether the standard radiographic angles suggested in textbooks are optimal for an Asian population since Asian groups have lower lumbar lordosis. This study found a 35° angle to be the most effective angle for lumbar oblique X-ray imaging. Additionally, the potential for a customized positioning auxiliary device was examined to improve image quality and reduce patient discomfort in cost-sensitive healthcare settings like Taiwan’s single-payer system. Methods: A total of 100 participants underwent lumbar oblique radiography using a specially designed footboard with angle markings. Radiologists evaluated 600 images based on waist-to-hip ratio (WHR) and body mass index to identify the optimal angulation for various body types. Results: For individuals with a WHR of 0.85, a 35° angle provided superior image quality, while 45° was more effective for slimmer patients. This optimized approach indicates the cost-effectiveness and diagnostic value of traditional X-ray imaging. Conclusions: The 35° angulation standardizes lumbar X-ray imaging for an Asian cohort, reducing repeat scans and improving accuracy. Using a positioning device further enhances image quality and patient comfort, supporting the clinical utility of traditional radiography in resource-limited environments.