bluuebunny/crossref_metadata_2025 huggingface dataset:
Adaptation and Dissemination of a National Cancer Institute HPV Vaccine Evidence-Based Cancer Control Program to the Social Media Messaging Environment
Authors: Hopfer Suellen , Phillips Kalani Kieu-Diem , Weinzierl Maxwell , Vasquez Hannah E. , Alkhatib Sarah , Harabagiu Sanda M.
Published: July 2022

Social media offers a unique opportunity to widely disseminate HPV vaccine messaging to reach youth and parents, given the information channel has become mainstream with 330 million monthly users in the United States and 4.2 billion users worldwide. Yet, a gap remains on how to adapt evidence-based vaccine interventions for the in vivo competitive social media messaging environment and what strategies to employ to make vaccine messages go viral. Push-pull and RE-AIM dissemination frameworks guided our adaptation of a National Cancer Institute video-based HPV vaccine cancer control program, the HPV Vaccine Decision Narratives, for the social media environment. We also aimed to understand how dissemination might differ across three platforms, namely Instagram, TikTok, and Twitter, to increase reach and engagement. Centering theory and a question-answer framework guided the adaptation process of segmenting vaccine decision story videos into shorter coherent segments for social media. Twelve strategies were implemented over 4 months to build a following and disseminate the intervention. The evaluation showed that all platforms increased following, but Instagram and TikTok outperformed Twitter on impressions, followers, engagement, and reach metrics. Although TikTok increased reach the most (unique accounts that viewed content), Instagram increased followers, engagement, and impressions the most. For Instagram, the top performer, six of 12 strategies contributed to increasing reach, including the use of videos, more than 11 hashtags, COVID-19 hashtags, mentions, and follow-for-follow strategies. This observational social media study identified dissemination strategies that significantly increased the reach of vaccine messages in a real-world competitive social media messaging environment. Engagement presented greater challenges. Results inform the planning and adaptation considerations necessary for transforming public health HPV vaccine interventions for social media environments, with unique considerations depending on the platform.
Differences in structural connectivity between diabetic and psychological erectile dysfunction revealed by network-based statistic: A diffusion tensor imaging study
Authors: Chen Jianhuai , Wu Jindan , Huang Xinfei , Sun Rui , Xiang Ziliang , Xu Yan , Chen Shi , Xu Weilong , Yang Jie , Chen Yun
Published: July 2022

Introduction

Type 2 diabetes mellitus (T2DM) has been found to be associated with abnormalities of the central and peripheral vascular nervous system, which were considered to be involved in the development of cognitive impairments and erectile dysfunction (ED). In addition, altered brain function and structure were identified in patients with ED, especially psychological ED (pED). However, the similarities and the differences of the central neural mechanisms underlying pED and T2DM with ED (DM-ED) remained unclear.

Methods

Diffusion tensor imaging data were acquired from 30 T2DM, 32 ED, and 31 DM-ED patients and 47 healthy controls (HCs). Then, whole-brain structural networks were constructed, which were mapped by connectivity matrices (90 × 90) representing the white matter between 90 brain regions parcellated by the anatomical automatic labeling template. Finally, the method of network-based statistic (NBS) was applied to assess the group differences of the structural connectivity.

Results

Our NBS analysis demonstrated three subnetworks with reduced structural connectivity in DM, pED, and DM-ED patients when compared to HCs, which were predominantly located in the prefrontal and subcortical areas. Compared with DM patients, DM-ED patients had an impaired subnetwork with increased structural connectivity, which were primarily located in the parietal regions. Compared with pED patients, an altered subnetwork with increased structural connectivity was identified in DM-ED patients, which were mainly located in the prefrontal and cingulate areas.

Conclusion

These findings highlighted that the reduced structural connections in the prefrontal and subcortical areas were similar mechanisms to those associated with pED and DM-ED. However, different connectivity patterns were found between pED and DM-ED, and the increased connectivity in the frontal–parietal network might be due to the compensation mechanisms that were devoted to improving erectile function.
Spindle pole body component 25 and platelet-derived growth factor mediate crosstalk between tumor-associated macrophages and prostate cancer cells
Authors: Cui Feilun , Xu Zhipeng , Hu Jianpeng , Lv Yumei
Published: July 2022

Tumor-associated macrophages (TAMs) are involved in the growth of prostate cancer (PrC), while the molecular mechanisms underlying the interactive crosstalk between TAM and PrC cells remain largely unknown. Platelet-derived growth factor (PDGF) is known to promote mesenchymal stromal cell chemotaxis to the tumor microenvironment. Recently, activation of spindle pole body component 25 (SPC25) has been shown to promote PrC cell proliferation and is associated with PrC stemness. Here, the relationship between SPC25 and PDGF in the crosstalk between TAM and PrC was investigated. Significant increases in both PDGF and SPC25 levels were detected in PrC specimens compared to paired adjacent normal prostate tissues. A significant correlation was detected between PDGF and SPC25 levels in PrC specimens and cell lines. SPC25 increased PDGF production and tumor cell growth in cultured PrC cells and in xenotransplantation. Mechanistically, SPC25 appeared to activate PDGF in PrC likely through Early Growth Response 1 (Egr1), while the secreted PDGF signaled to TAM through PDGFR on macrophages and polarized macrophages, which, in turn, induced the growth of PrC cells likely through their production and secretion of transforming growth factor β1 (TGFβ1). Thus, our data suggest that SPC25 triggers the crosstalk between TAM and PrC cells via SPC25/PDGF/PDGFR/TGFβ1 receptor signaling to enhance PrC growth.
Biodiversity of coastal epibenthic macrofauna in Eastern Canadian Arctic: Baseline mapping for management and conservation
Authors: Gianasi Bruno L. , McKindsey Christopher W. , Archambault Philippe , Simard Nathalie , Howland Kimberly L.
Published: July 2022

Arctic ecosystems are changing rapidly due to global warming, industrial development, and economic growth. However, the ecological consequences for these ecosystems are difficult to predict due to limited knowledge on species abundance, distribution, and biodiversity patterns. This study evaluated the diversity and assemblage composition of epibenthic macrofauna in shallow coastal areas from five Eastern Arctic locations with increasing economic and shipping activity. Benthic trawls (n=198) were conducted in nearshore coastal habitats of Anaktalak Bay (Labrador), Churchill (Manitoba), Deception Bay (Quebec), Iqaluit (Nunavut), and Milne Inlet (Nunavut), at depths between 3 and 30 m. Diversity and assemblage composition were compared at various taxonomic levels from phylum to species and correlations with broad oceanographic variables were investigated to identify potential drivers of biodiversity. The spatial variability of benthic assemblages was also assessed within each study location. A total of 297,417 macroinvertebrates was identified, belonging to 900 taxa. Abundance and taxonomic richness were highest in Anaktalak Bay. Shannon-Wiener diversity was higher in Anaktalak Bay, Iqaluit, and Milne Inlet than in Churchill and Deception Bay. Churchill showed the lowest diversity metrics among locations. No relationships were observed between diversity and depth, chlorophyll-a, particulate organic carbon, sea surface temperature, or sea ice duration. Assemblages differed among locations at all taxonomic levels, with the highest dissimilarities at the species level; however, dispersion of samples within-groups was significant, suggesting that factors other than locations (e.g., habitat type) influence assemblage composition. While Churchill, Deception Bay, and Iqaluit showed distinct local spatial patterns in diversity metrics and assemblage composition, no patterns were detected in Anaktalak Bay and Milne Inlet. This study represents one of the largest systematic assessments of coastal epibenthic biodiversity in the Canadian Arctic. It identifies patterns of biodiversity and assemblage composition and provides a baseline for studies of community change and the development of informed management and conservation strategies for Arctic coastal ecosystems.
Insights from tyrosinase into the impacts of modified morphology of calcium carbonate on the nacre formation of pearl oysters
Authors: Xiong Xinwei , Cao Yanfei , Li Zhixin , Huang Ronglian , Jiao Yu , Zhao Liqiang , Du Xiaodong , Zheng Zhe
Published: July 2022

Tyrosinase is a type-3 copper protein with six conserved histidine residues within the copper-binding sites. It participates in mollusk nacre formation. Here, we identified nacreous-layer-specific tyrosinases (NLSTyr) from Pinctada fucata martensii (PmTyr-4 and PmTyr-6), as well as their homologs in Pinctada maxima (PmaxTyr and PmaxTyr4) and Pinctada margaritifera (PmarTyr and PmarTyr-4), which encoded tyrosinases without the six conserved histidine residues within the copper-binding sites. PmTyr-4 and PmTyr-6 mRNAs were spatially concentrated in the mantle central and pearl sac, which are the organs responsible for nacre formation. During shell regeneration and pearl formation, PmTyr-4 and PmTyr-6 were also significantly highly expressed in the mantle and pearl sac. RNA interference showed that PmTyr-4 participated in nacreous-layer formation. The recombinant protein of PmTyr-4 (rPmTyr-4) inhibited the calcium carbonate precipitation rate. Correspondingly, calcium carbonate crystallization assay showed that the aragonite crystals of the rPmTyr-4 group were smaller than those of the control group. Moreover, the calcite and aragonite morphologies of the rPmTyr-4 group were modified compared with the control group. These results suggested that NLSTyr in pearl oyster inhibited calcium carbonate precipitation and affected crystal morphologies during nacre formation. Our findings provided new insights into the evolution and function gain of tyrosinase in Mollusk.
RETRACTED: Substantial seagrass blue carbon pools in the southwestern Baltic Sea include relics of terrestrial peatlands
Authors: Stevenson Angela , Ó Corcora Tadhg C. , Hukriede Wolfgang , Schubert Philipp R. , Reusch Thorsten B. H.
Published: July 2022

Seagrass meadows have a disproportionally high organic carbon (Corg) storage potential within their sediments and thus can play a role in climate change mitigationviatheir conservation and restoration. However, high spatial heterogeneity is observed in Corg, with wide differences seen globally, regionally, and even locally (within a seagrass meadow). Consequently, it is difficult to determine their contributions to the national remaining carbon dioxide (CO2) budget without introducing a large degree of uncertainty. To address this spatial heterogeneity, we sampled 20 locations across the German Baltic Sea to quantify Corgstocks and sources inZostera marinaseagrass-vegetated and adjacent unvegetated sediments. To predict and integrate the Corginventory in space, we measured the physical (seawater depth, sediment grain size, current velocity at the seafloor, anthropogenic inputs) and biological (seagrass complexity) environments to determine regional and local drivers of Corgvariation. Here, we show that seagrass meadows in Germany constitute a significant Corgstock, storing on average 7,785 g C/m2, 13 times greater than meadows from other parts of the Baltic Sea, and fourfold richer than adjacent unvegetated sediments. Stocks were highly heterogenous; they differed widely between (by 10-fold) and even within (by 3- to 55-fold) sites. Regionally, Corgwas controlled by seagrass complexity, fine sediment fraction, and seawater depth. Autochthonous material contributed to 78% of the total Corgin seagrass-vegetated sediments, and the remaining 22% originated from allochthonous sources (phytoplankton and macroalgae). However, relic terrestrial peatland material, deposited approximately 6,000 years BP during the last deglaciation, was an unexpected and significant source of Corg. Collectively, German seagrasses in the Baltic Sea are preventing 8.14 Mt of future CO2emissions. Because Corgis mostly produced on-site and not imported from outside the meadow boundaries, the richness of this pool may be contingent on seagrass habitat health. Disturbance of this Corgstock could act as a source of CO2emissions. However, the high spatial heterogeneity warrants site-specific investigations to obtain accurate estimates of blue carbon and a need to consider millennial timescale deposits of Corgbeneath seagrass meadows in Germany and potentially other parts of the southwestern Baltic Sea.
An Updated Genome Assembly Improves Understanding of the Transcriptional Regulation of Coloration in Midas Cichlid
Authors: Lv Yunyun , Li Yanping , Liu Yi , Wen Zhengyong , Yang Yexin , Qin Chuanjie , Shi Qiong , Mu Xidong
Published: July 2022

Midas cichlid (Amphilophus citrinellus), a popular aquarium fish, attracts extensive attention from worldwide biologists mainly due to its morphological polymorphism (dark versus gold). Continuous efforts have therefore been paid to address mechanisms of its coloration variants, while it is far away from the detailed illustration of a clear regulatory network. Some limits may come from the absence of a high-quality genome assembly and a relatively accurate gene set. In this study, we sequenced about 149 Gb of nucleotide sequences of Midas cichlid, generating a genome assembly with a total size of 933.5 Mb, which exhibits a good genome continuity with a contig N50 of 10.5 Mb. A total of 25,911 protein-coding genes were annotated and about 90% completeness was achieved, which helps to build a good gene pool for understanding expressional differences of color variation. With the assistance of the final gene set, we identified a total of 277 differential expressional genes (DEGs), of which 97 up- and 180 downregulated were determined in dark-vs-gold comparisons. Two protein-protein interaction (PPI) networks were constructed from these DEGs, and three key functional modules were classified. Hub genes within each module were evaluated, and we found that the third key module contains tyrp1b, oca2, pmela, tyr, and slc24a5, which were previously proven to be associated with melanin formation. Two downregulated DEGs (myl1 and pgam2) in the first key module may be involved in muscle movement and spermatogenesis, implying that certain side effects could result from the morphological polymorphism. The first key module, consisting of proteins encoded by upregulated DEGs that were associated with MAPK signaling, Toll-like receptor signaling, and gonadotropin-releasing hormone pathways, may contribute to a negative upstream regulation or downstream influence on melanin biosynthesis. Taken together, our new genome assembly and gene annotation of Midas cichlid provide a high-quality genetic resource for biological studies on this species, and the newly identified key networks and hub genes in dark-vs-gold comparisons enhance our understanding of the transcriptional regulatory mechanisms underlying coloration changes not only in Midas cichlid but also in other fishes from freshwater to marine ecosystems.
A survey of Australian and New Zealand medical parents' experiences of infertility, pregnancy, and parenthood
Authors: Kevric Jasmina , Suter Katherine , Hodgson Russell , Chew Grace
Published: July 2022

Objective

To describe the incidence of infertility, pregnancy complications, and breastfeeding practices among Australian and New Zealand doctors and identify factors associated with increased pregnancy complication rates.

Methods

A survey of ANZ doctors using an online questionnaire during November 2021.

Results

One thousand ninety-nine completed responses were received. The median age of female doctors at the time of their first child was 32.4. Fertility testing was undertaken by 37%, with 27% having in vitro fertilization. More than 60% of respondents delayed family planning due to work. Pregnancy loss occurred in 36% of respondents, and 50% suffered a pregnancy complication. There were significant differences between specialists, with surgeons working longer hours before and after pregnancy, but having greater access to maternity leave than general practitioners.

Conclusion

Female doctors delay starting and completing their family due to work-related demands and structural biases in career progression, which may result in higher infertility and pregnancy complication rates.
The heat shock protein 20 gene editing suppresses mycelial growth of Botryosphaeria dothidea and decreases its pathogenicity to postharvest apple fruits
Authors: Huang Yonghong , Liu Junping , Li Jinghui , Sun Meng , Duan Yanxin
Published: July 2022

Apple ring rot caused by Botryosphaeria dothidea is an essential and prevalent disease in the apple orchard in China. Our previous study demonstrated that dimethyl trisulfide (DT) from Chinese leek (Allium tuberosum) significantly suppressed the mycelial growth of B. dothidea and inhibited the incidence of apple ring rot postharvest. However, the mechanism underlying the inhibitory role of DT against B. dothidea is not fully understood. Comparing the control and the DT-treated B. dothidea mycelial transcriptomes revealed that heat shock protein 20 (Hsp20) strongly responded to DT treatment. This study identified four Hsp20 genes throughout the B. dothidea genome (BdHsp20_1-4). Each BdHsp20 gene had a conserved ACD with a variable N-terminal region and a short C-terminal extension. The segmental duplication event has contributed to the expansion of the BdHsp20 gene family. Compared to the wild-type strain, the CRISPR/Cas9 gene-edited BdHsp20 mutant (ΔBdHsp20) decreased the mycelial growth by 55.95% and reduced the disease symptom in postharvest apple fruit by 96.34%. However, the BdHsp20 complemented strain (ΔBdHsp20_C) significantly restored the growth and pathogenicity, which suggested that the BdHsp20 gene was closely involved in the growth and pathogenicity of B. dothidea. This study would accelerate the exploration of the molecular mechanism of the inhibitory effect of DT against B. dothidea and also provide new insights for the management of apple ring rot disease.
Screening and identification of nucleocapsid protein-nanobodies that inhibited Newcastle disease virus replication in DF-1 cells
Authors: Fan Wenqi , Ji Pinpin , Sun Xuwen , Kong Min , Zhou Ning , Zhang Qiang , Wang Ying , Liu Qianqian , Li Xiaoxuan , Zhou En-Min , Zhao Qin , Sun Yani
Published: July 2022

Newcastle disease (ND) is an acute and highly contagious infectious disease found in poultry. Although commercial ND virus (NDV) vaccines are universally used, some case reports persistently documented vaccination failure. Therefore, novel strategies are still required to control the occurrence of the disease in chickens. Recently, nanobodies (Nbs), which have the advantages of small molecular weight and low production costs, have been shown to be promising therapeutics against viral infection. In the present study, a total of 16 Nbs against NDV nucleocapsid protein (NP) were screened from two libraries against NDV using phage display technology. Of the 16 screened Nbs, eight were prevented from binding to NDV NP protein through administering positive chicken sera for anti-NDV antibodies, indicating that the epitopes recognized by these eight Nbs were able to induce the immune response after the chickens were infected with NDV stock. Subsequently, transfection assay, construction of recombinant DF-1 cells capable of expressing different nanobodies and viral inhibition assay were used to screen the nanobodies inhibiting NDV replication. The results demonstrated that Nb18, Nb30, and Nb88 significantly inhibited the replication of Class I and different genotypes of Class II NDV strains in DF-1 cells when they were expressed in the cytoplasm. Collectively, these nanobodies provided new tools for researching the functions of NDV NP protein and may be used as a novel strategy for designing drugs against NDV infection in chickens.
Reduced Expression of TMEM16A Impairs Nitric Oxide-Dependent Cl− Transport in Retinal Amacrine Cells
Authors: Rodriguez Tyler Christopher , Zhong Li , Simpson Hailey , Gleason Evanna
Published: July 2022

Postsynaptic cytosolic Cl− concentration determines whether GABAergic and glycinergic synapses are inhibitory or excitatory. We have shown that nitric oxide (NO) initiates the release of Cl− from acidic internal stores into the cytosol of retinal amacrine cells (ACs) thereby elevating cytosolic Cl−. In addition, we found that cystic fibrosis transmembrane conductance regulator (CFTR) expression and Ca2+ elevations are necessary for the transient effects of NO on cytosolic Cl− levels, but the mechanism remains to be elucidated. Here, we investigated the involvement of TMEM16A as a possible link between Ca2+ elevations and cytosolic Cl− release. TMEM16A is a Ca2+-activated Cl− channel that is functionally coupled with CFTR in epithelia. Both proteins are also expressed in neurons. Based on this and its Ca2+ dependence, we test the hypothesis that TMEM16A participates in the NO-dependent elevation in cytosolic Cl− in ACs. Chick retina ACs express TMEM16A as shown by Western blot analysis, single-cell PCR, and immunocytochemistry. Electrophysiology experiments demonstrate that TMEM16A functions in amacrine cells. Pharmacological inhibition of TMEM16A with T16inh-AO1 reduces the NO-dependent Cl− release as indicated by the diminished shift in the reversal potential of GABAA receptor-mediated currents. We confirmed the involvement of TMEM16A in the NO-dependent Cl− release using CRISPR/Cas9 knockdown of TMEM16A. Two different modalities targeting the gene for TMEM16A (ANO1) were tested in retinal amacrine cells: an all-in-one plasmid vector and crRNA/tracrRNA/Cas9 ribonucleoprotein. The all-in-one CRISPR/Cas9 modality did not change the expression of TMEM16A protein and produced no change in the response to NO. However, TMEM16A-specific crRNA/tracrRNA/Cas9 ribonucleoprotein effectively reduces both TMEM16A protein levels and the NO-dependent shift in the reversal potential of GABA-gated currents. These results show that TMEM16A plays a role in the NO-dependent Cl− release from retinal ACs.
Efficacy and safety of acupuncture combined with auricular acupressure for smoking cessation: A study protocol of a multicentre, randomized, controlled clinical trial
Authors: Zeng Jinchun , Liao Yizu , Wei Xiaojing , Chen Guangxian , Cai Zibin , Chen Min , Gou Yanhua , Lin Guohua
Published: July 2022

Background

Nicotine dependence is an addictive behavioral disease facilitated by habitually smoking cigarettes. In many countries, acupuncture and auricular acupressure have attracted growing attention as complementary or alternative treatments for smoking cessation; however, there is a lack of rigorous randomized, controlled studies evaluating the combination of these two interventions specifically for smoking cessation. The aim of this study is to evaluate the efficacy and safety of using acupuncture combined with auricular acupressure (A&AA) to increase the rates of smoking cessation and ultimately reduce the rates of relapse.

Methods

This is a multicentre, prospective, parallel, randomized, controlled trial. A total of 360 patients with severe nicotine dependence will be randomized into test (A&AA) or control (nicotine replacement therapy, NRT) groups. The test group will be treated with A&AA twice weekly, while the control group will use an NRT patch daily. All treatments will be administered for 8 weeks, with a follow-up period of 4 months. The primary outcome will be the smoking abstinence rate at week 24, with a combined safety assessment. The secondary outcomes will be smoking cessation rates at other timepoints, saliva cortisone test results, and scores on the Fagerstrom Test for Nicotine Dependence, the Autonomy over Tobacco Scale, the Hamilton Anxiety Rating Scale, the Self-rating Anxiety Scale, and the Pittsburgh Sleep Quality Index. The cost of treatment will also be used to evaluate the economic effects of different smoking cessation interventions. Statistical analysis on the data collected from both the intention-to-treat (all randomly assigned patients) and per-protocol (patients who complete the trial without any protocol deviations) patients, will be performed using the statistical software package, IBM SPSS 27.0.

Discussion

This study will provide rigorous clinical evidence evaluating the efficacy and safety of using A&AA as a smoking cessation therapy.

Trial registration

Chinese Clinical Trial Registry (Registration number: ChiCTR1900028371).
Automated measurement of the disc-fovea angle based on DeepLabv3+
Authors: Zheng Bo , Shen Yifan , Luo Yuxin , Fang Xinwen , Zhu Shaojun , Zhang Jie , Wu Maonian , Jin Ling , Yang Weihua , Wang Chenghu
Published: July 2022

Purpose

To assess the value of automatic disc-fovea angle (DFA) measurement using the DeepLabv3+ segmentation model.

Methods

A total of 682 normal fundus image datasets were collected from the Eye Hospital of Nanjing Medical University. The following parts of the images were labeled and subsequently reviewed by ophthalmologists: optic disc center, macular center, optic disc area, and virtual macular area. A total of 477 normal fundus images were used to train DeepLabv3+, U-Net, and PSPNet model, which were used to obtain the optic disc area and virtual macular area. Then, the coordinates of the optic disc center and macular center were obstained by using the minimum outer circle technique. Finally the DFA was calculated.

Results

In this study, 205 normal fundus images were used to test the model. The experimental results showed that the errors in automatic DFA measurement using DeepLabv3+, U-Net, and PSPNet segmentation models were 0.76°, 1.4°, and 2.12°, respectively. The mean intersection over union (MIoU), mean pixel accuracy (MPA), average error in the center of the optic disc, and average error in the center of the virtual macula obstained by using DeepLabv3+ model was 94.77%, 97.32%, 10.94 pixels, and 13.44 pixels, respectively. The automatic DFA measurement using DeepLabv3+ got the less error than the errors that using the other segmentation models. Therefore, the DeepLabv3+ segmentation model was finally chosen to measure DFA automatically.

Conclusions

The DeepLabv3+ segmentation model -based automatic segmentation techniques can produce accurate and rapid DFA measurements.
How does the use of simultaneous contrast illusion on product-background color combination nudge consumer behavior? A behavioral and event-related potential study
Authors: Peng Minjing , Tong Yao , Xu Zhicheng , Jiang Linli , Huang Haiyang
Published: July 2022

Color, as one of the most critical visual factors influencing consumer decisions, has been widely used in e-commerce marketing. However, the effects of product-background saturation combination on consumers’ willingness to purchase products with different heaviness attributes (e.g., heaviness-positive products or heaviness-negative products) have not been conclusively determined. The current study demonstrated the effects of product-background saturation combination on product heaviness perception and its downstream consequences. Based on behavioral method, study 1 showed that a patch of color placed in a pale background (the saturation of the background is lower than the saturation of the color patch) was perceived as visually heavier than that in a colorful background (the saturation of the background is higher than the saturation of the color patch). Study 2 applied event-related potentials (ERPs) method to explore the underlying neural mechanisms of how the interactions between the presentation modes and the product types affect consumer decisions. Behaviorally, compared to the colorful background, the pale background would lead to a higher purchase rate for the heaviness-positive products, whereas the opposite results were found for the heaviness-negative products. Furthermore, for both the heaviness-positive and heaviness-negative products, a shorter reaction time would be observed in the pale background condition than in the colorful background condition. Neurophysiologically, the pale background would result in smaller N2 component and larger P3 component compared to the colorful background for the heaviness-positive products, while the reverse held for the heaviness-negative products. Smaller N2 component implies decreased perceptual conflicts and larger P3 component implies increased decision confidence, suggesting that e-retailers should present heaviness-positive products with pale backgrounds and heaviness-negative products with colorful backgrounds.
Parkinson’s disease detection based on multi-pattern analysis and multi-scale convolutional neural networks
Authors: Qiu Lina , Li Jianping , Pan Jiahui
Published: July 2022

Parkinson’s disease (PD) is a complex neurodegenerative disease. At present, the early diagnosis of PD is still extremely challenging, and there is still a lack of consensus on the brain characterization of PD, and a more efficient and robust PD detection method is urgently needed. In order to further explore the features of PD based on brain activity and achieve effective detection of PD patients (including OFF and ON medications), in this study, a multi-pattern analysis based on brain activation and brain functional connectivity was performed on the brain functional activity of PD patients, and a novel PD detection model based on multi-scale convolutional neural network (MCNN) was proposed. Based on the analysis of power spectral density (PSD) and phase-locked value (PLV) features of multiple frequency bands of two independent resting-state electroencephalography (EEG) datasets, we found that there were significant differences in PSD and PLV between HCs and PD patients (including OFF and ON medications), especially in the β and γ bands, which were very effective for PD detection. Moreover, the combined use of brain activation represented by PSD and functional connectivity patterns represented by PLV can effectively improve the performance of PD detection. Furthermore, our proposed MCNN model shows great potential for automatic PD detection, with cross-validation accuracy, sensitivity, specificity, and area under the receiver operating characteristic curve all above 99%. Our study may help to further understand the characteristics of PD and provide new ideas for future PD diagnosis based on spontaneous EEG activity.
Cerebral blood flow in adolescents with drug-naive, first-episode major depressive disorder: An arterial spin labeling study based on voxel-level whole-brain analysis
Authors: Xiong Ying , Chen Rong-Sheng , Wang Xing-Yu , Li Xiao , Dai Lin-Qi , Yu Ren-Qiang
Published: July 2022

Purpose

The major depressive disorder (MDD) can be a threat to the health of people all over the world. Although governments have developed and implemented evidence-based interventions and prevention programs to prevent MDD and maintain mental health in adolescents, the number of adolescents with this condition has been on the rise for the past 10 years.

Methods

A total of 60 adolescents were recruited, including 32 drug-naive adolescents with first-episode MDD and 28 healthy controls (HCs). Alterations in the intrinsic cerebral activity of the adolescents with MDD were explored using arterial spin labeling (ASL) while differences in the regional cerebral blood flow (rCBF) of the two groups were assessed based on voxel-based whole-brain analysis. Finally, correlations between the regional functional abnormalities and clinical variables were investigated for adolescents with MDD.

Results

Compared with HCs, MDD patients had a lower rCBF in the left triangular part of the inferior frontal gyrus (IFGtriang) but a higher one in the right Precental gyrus (PreCG). Negative correlations were also noted between the CBF in the left IFGtriang and the Hamilton depression scale (HAMD) scores of MDD patients.

Conclusion

Elucidating the neurobiological features of adolescent patients with MDD is important to adequately develop methods that can assist in early diagnosis, precaution and intervention.
Financial Wellbeing and Quality of Life Among a Sample of the Lebanese Population: The Mediating Effect of Food Insecurity
Authors: Karam Joanne , Haddad Chadia , Sacre Hala , Serhan Mireille , Salameh Pascale , Jomaa Lamis
Published: July 2022

Background

Lebanon is undergoing multiple overlapping crises, affecting the food security, financial well-being, and quality of life (QOL) of its residents.

Objective

The primary objective was to assess the food insecurity (FI) status of a sample of the Lebanese population. The second objective was to explore factors related to QOL parameters and evaluate the mediating effect of food security between financial well-being and QOL.

Methods

The study was cross-sectional and enrolled 412 participants recruited online using the snowball sampling technique. The survey included questions related to sociodemographic and economic characteristics of Lebanese households and validated scales to assess FI, QOL measures, financial well-being, and fear of COVID-19.

Results

Almost 43% of the study participants reported being food insecure, with 31% experiencing mild FI, 10% moderate FI, and 1.5% severe FI. Compared to food-insecure participants, food secure participants had a significantly higher income (58.5% vs. 39.2%, p < 0.001), a university education level (96.6% vs. 88.1%, p = 0.002), an average perceived financial status (83.9% vs. 65.9%), higher financial well-being scores (5.14 vs. 3.19, p < 0.001), and lower crowding index (0.94 ± 0.4 vs. 1.09, p = 0.002). Multivariate analysis showed that FI was not associated with physical (Beta = −1.48, 95% CI: −3.10; 0.13) and mental (Beta = −1.46, 95% CI −3.68; 0.75) QOL, after adjusting for other demographic and socioeconomic correlates. This association remained non-significant when introducing the financial well-being variable to the model. Mediation analyses showed that the FI variable mediated the association between financial well-being and physical QOL (Beta = 0.19, 95% CI: 0.02; 0.36), but not the mental QOL (Beta = −0.02, 95% CI: −0.20; 0.14).

Conclusion

Food insecurity was prevalent in our study sample, and it mediated the association between financial well-being and physical, but not mental, QOL parameters. These findings call for evidence-based policies and programs to help improve the food security and well-being of Lebanese households amidst these unprecedented circumstances.
Editorial: Immunotherapy in renal cell carcinoma
Authors: Gruenwald Viktor , Storkus Walter J.
Published: July 2022

This collection contains 10 reports published In Frontiers in Oncology between August 2020 and March 2022 broadly focused on the immunobiology of renal cell carcinoma (RCC), the impact of immunotherapy in the setting of RCC, and the identification of biomarkers that are prognostic of RCC patient outcomes and response to immunotherapy.
Nanoporous silica gel can compete with the flower stigma in germinating and attracting pollen tubes
Authors: Chichiriccò Giuseppe , Poma Anna , Pace Loretta
Published: July 2022

To find nanoporous substrates with hydrodynamic properties useful for pollen hydration and germination, we used the glassy Silica gel and Vycor scales and pollen with different morphological and physiological traits, that ofCrocus vernus, and that ofNarcissus poeticus. Forin vitrotests, the scales were spread on microscope slides, hand pollinated, and incubated. Pollen germination was evaluated with the stereomicroscope and the tube growth was explored with scanning electron microscopy (SEM). Thein vivotests were carried out by sprinkling the stigmas of theCrocusplants with Silica gel scales and immediately after having pollinated them by hand, the plants were incubated. Three hours later, the stigmas were removed and treated for observation with SEM.In vitrothe pollen of both species germinated on Silica gel with percentages similar to those of thein vivoandin vitrocontrols, accumulating fibrillary material at the interface. The tubes grew perpendicular to the surface of the scales, trying to penetrate the scales to the point of flattening with the apex. OnCrocusstigmas sprinkled with Silica gel scales, pollen developed tubes that grew to the scales rather than penetrating the papillae. The results underline the close interaction of pollen with nanoporous artificial material, so much so that its pollen tubes are attracted to the Silica scales more than to the stigma papillae that arises from a mechanism of natural selection.
Relationship Between Childhood Sensory Processing Differences and Quality of Life as Adults
Authors: May-Benson Teresa A. , Teasdale Alison , Easterbrooks-Dick Olivia
Published: July 2022

Research suggests a relationship between sensory processing, motor skills and quality of life (QOL) in a variety of clinical populations of adults and children. There have been no studies which investigated the relationship of childhood sensory processing and integration and related motor performance (sensori-motor) patterns identified using an Ayres Sensory Integration® (ASI) frame of reference and later QOL of those children as adults. This longitudinal follow-up study examined this relationship. Adult QOL was also examined in relation to current adult sensori-motor patterns. Fifty-three adults who received occupational therapy services as children, were identified as having sensori-motor difficulties at that time and completed a sensory history and a quality-of-life measure as adults participated. Measures included the OTA the Koomar Center Sensory History (SXHX), Adult/Adolescent Sensory History (ASH), and the World Health Organization Quality of Life-Brief (WHOQOL-BREF). MANCOVA found that Total childhood sensori-motor scores had a small relationship to Physical Health QOL as adults that approached significance. Pearson Correlations found that adults with childhood sensori-motor challenges who report sensori-motor challenges as adults had a moderate significant relationship among overall sensori-motor functioning and Physical Health (r = −0.56, p = 0.018). Visual (r = −0.76, p = 0.001), movement (vestibular; r = −0.48, p = 0.042) and tactile processing (r = −0.63, p = 0.008) had moderate to large significant relationships with Physical Health. Visual processing (r = −0.54, p = 0.024) was also significantly related to Psychological Health. Motor Coordination trended to significance for Physical Health (r = −0.42) and Psychological Health (r = −0.41). Conversely, adults who reported typical sensori-motor scores as adults, despite childhood sensori-motor challenges, had a good QOL. Furthermore, similar to previous research, there was a relationship between current visual, movement (vestibular) and tactile (touch) sensory processing and adult Physical and Psychological Health. A multivariate linear regression found Sensory Discrimination and Modulation accounted for one-quarter of the variance in QOL in adults with only Discrimination being statistically significant. Therefore, it is important to consider childhood sensori-motor function as well as adult functioning when examining QOL. Further, heretofore unexamined Sensory Discrimination was found to play a role in adult QOL.
Construction of self-learning classroom history teaching mode based on human-computer interaction emotion recognition
Authors: Ji Changwei , Zhao Shuyan
Published: July 2022

Due to the continuous epidemic in recent years, the traditional teaching mode of history classroom has been gradually replaced by the teaching mode of self-learning classroom. The teaching mode of autonomous learning classroom has become a popular teaching mode in recent years. However, in the autonomous learning classroom under the current history teaching mode, the lecturer cannot always pay attention to the various states of the students. It is also difficult to understand and receive the information the teacher wants to convey in real time. For this reason, human-computer interaction emotion recognition technology has been proposed and developed. In order to construct and realize the teaching mode of self-learning classroom history, this paper studies the emotion recognition technology of human-computer interaction. The research results show that the introduction of human-computer interaction emotion recognition technology into the construction of autonomous learning classroom teaching mode can fully understand students' emotional behavior. It improves the accuracy of students' emotion recognition by 2.67%, enables students to maintain a good learning motivation, and make reasonable plans and arrangements for the historical time and progress of learning. At the same time, it enhances the history teaching intensity and autonomous learning ability, and improves the original single learning mode. By establishing a new teaching-teacher-student relationship, it creates a good and active autonomous classroom atmosphere.
Evaluating the impact and cost-effectiveness of chlamydia management strategies in Hong Kong: A modeling study
Authors: Montes-Olivas Sandra , Ozten Yaz , Homer Martin , Turner Katy , Fairley Christopher K. , Hocking Jane S. , Tse Desiree , Verschueren van Rees Nicolas , Wong William C. W. , Ong Jason J.
Published: July 2022

Objectives

To illustrate the epidemiologic and cost-effectiveness impact of shifting the focus from population-based screening toward a targeted management approach for genital chlamydia infection.

Design

Modeling study, implementing an individual-based, stochastic, dynamic network model.

Setting

Hong Kong.

Population

A hypothetical sample network of 10,000 people with a partnership distribution based on Hong Kong's sexually active population of reproductive age (age 18–49 years).

Interventions

In this study, we present several scenarios with different implementations of universal vs. targeted screening (based on partner numbers). We also explored the impact of (1) screening only, (2) screening plus expedited partner therapy, and (3) screening plus partner testing.

Primary outcome measures

Change of chlamydia prevalence before and after implementing the different strategies. The cost-effectiveness analysis reports total direct cost from a health provider perspective, the QALYs gained, and incremental cost-effectiveness ratios (ICER).

Results

In comparing the effects of universal screening only and targeted screening of the high-risk population, the mean prevalence during the 10th year of intervention was 2.75 ± 0.30% and 2.35 ± 0.21%, respectively (compared with 3.24 ± 0.30% and 3.35 ± 0.21% before the interventions, respectively). The addition of contact tracing to the latter targeted screening scenario reduces the mean prevalence during the 10th year of intervention to 1.48 ± 0.13% (compared with 3.31 ± 0.33% at baseline) in the best-case of testing before treatment and maximal contact-tracing effectiveness (40%). Overall, the most effective scenarios were those for which interventions focused on the high-risk population defined by the number of partners, with contact tracing included. The ICER for targeted screening with contact tracing at 20% and 40% efficiency was $4,634 and $7,219 per QALY gained, respectively (10-year time horizon). Expedited partner therapy did not significantly impact overall chlamydia prevalence and caused overtreatment.

Conclusions

Our study suggests that targeted screening with strengthened contact tracing efforts is the most cost-effective strategy to reduce the prevalence of chlamydia in Hong Kong.
Health shocks and changes in preventive behaviors: Results from the China Health and Retirement Longitudinal Study
Authors: Zhang Peng , Jiang Hongli , Chen Wen
Published: July 2022

Background

China is facing the challenge of rising prevalence and ballooning burden of chronic non-communicable diseases (NCDs); however, the Chinese middle- and older-aged population considerably lack preventive behaviors. Health shocks (HS), widely defined as sudden health deterioration brought on by diseases or accidents, bring a “teachable moment” to motivate changes in preventive behaviors.

Objective

This study aims to examine the effect of HS on changes in preventive behaviors, including personal health practices and preventive care utilization.

Methods

HS was defined as any five chronic disease diagnoses (cancer, heart disease, stroke, diabetes, and hypertension). The impacts of HS on smoking, drinking, and exercise, physical examination were estimated. The panel data of 13,705 respondents were obtained from the latest two waves of the China Health and Retirement Longitudinal Study (CHARLS) in 2015 and 2018. A multilevel propensity score match difference-in-difference (multilevel PSM-DID) model was constructed.

Results

HS significantly decreased smoking (OR = 0.59, p < 0.05) and drinking (OR = 0.62, p < 0.01) and increased the utilization of auxiliary inspection in physical examination (OR = 1.19, p < 0.1). Major HS had significantly considerable and specific effects on reducing smoking and drinking (OR = 0.37 and 0.56, p < 0.01), while minor HS had relatively small effects on reducing smoking (OR = 0.74, p < 0.05) and drinking (OR = 0.69, p < 0.01), but extensive effects on initiating exercise (OR = 1.32, p < 0.05), physical examination (OR = 1.18, p < 0.1), and auxiliary inspection (OR = 1.30, p < 0.05).

Conclusion

After HS, there is a teachable moment to promote positive changes in preventive behaviors. Guided by the 5A's brief intervention model (Ask, Advise, Assess, Assist, Arrange), tailored interventions should be targeted at these populations to reduce the risk of the progression and complications of existing diseases, prevent the related comorbidity, and prolong the expected life-span.
Rethinking the A in FAIR Data: Issues of Data Access and Accessibility in Research
Authors: Shanahan Hugh , Bezuidenhout Louise
Published: July 2022

The FAIR data principles are rapidly becoming a standard through which to assess responsible and reproducible research. In contrast to the requirements associated with the Interoperability principle, the requirements associated with the Accessibility principle are often assumed to be relatively straightforward to implement. Indeed, a variety of different tools assessing FAIR rely on the data being deposited in a trustworthy digital repository. In this paper we note that there is an implicit assumption that access to a repository is independent of where the user is geographically located. Using a virtual personal network (VPN) service we find that access to a set of web sites that underpin Open Science is variable from a set of 14 countries; either through connectivity issues (i.e., connections to download HTML being dropped) or through direct blocking (i.e., web servers sending 403 error codes). Many of the countries included in this study are already marginalized from Open Science discussions due to political issues or infrastructural challenges. This study clearly indicates that access to FAIR data resources is influenced by a range of geo-political factors. Given the volatile nature of politics and the slow pace of infrastructural investment, this is likely to continue to be an issue and indeed may grow. We propose that it is essential for discussions and implementations of FAIR to include awareness of these issues of accessibility. Without this awareness, the expansion of FAIR data may unintentionally reinforce current access inequities and research inequalities around the globe.