bluuebunny/crossref_metadata_2025 huggingface dataset:
Successful diagnostic stewardship for Clostridioides difficile testing in pediatrics
Authors: Halabi Katia C. , Ross Barbara , Acker Karen P. , Cannon Jean-Marie , Messina Maria , Mangino Diane , Balzer Krystal , Hill-Ricciuti Alexandra , Green Daniel A. , Westblade Lars F. , Salvatore Christine M. , Saiman Lisa
Published: June 2022

Objective:

To reduce both inappropriate testing for and diagnosis of healthcare-onset (HO) Clostridioides difficile infections (CDIs).

Design:

We performed a retrospective analysis of C. difficile testing from hospitalized children before (October 2017–October 2018) and after (November 2018–October 2020) implementing restrictive computerized provider order entry (CPOE).

Setting:

Study sites included hospital A (a ∼250-bed freestanding children’s hospital) and hospital B (a ∼100-bed children’s hospital within a larger hospital) that are part of the same multicampus institution.

Methods:

In October 2018, we implemented CPOE. No testing was allowed for infants aged ≤12 months, approval of the infectious disease team was required to test children aged 13–23 months, and pathology residents’ approval was required to test all patients aged ≥24 months with recent laxative, stool softener, or enema use. Interrupted time series analysis and Mann-Whitney U test were used for analysis.

Results:

An interrupted time series analysis revealed that from October 2017 to October 2020, the numbers of tests ordered and samples sent significantly decreased in all age groups (P < .05). The monthly median number of HO-CDI cases significantly decreased after implementation of the restrictive CPOE in children aged 13–23 months (P < .001) and all ages combined (P = .003).

Conclusion:

Restrictive CPOE for CDI in pediatrics was successfully implemented and sustained. Diagnostic stewardship for CDI is likely cost-saving and could decrease misdiagnosis, unnecessary antibiotic therapy, and overestimation of HO-CDI rates.
ADDITIVE COVERS AND THE CANONICAL BASE PROPERTY
Authors: LOESCH MICHAEL
Published: June 2022

We give a new approach to the failure of the Canonical Base Property (CBP) in the so far only known counterexample, produced by Hrushovski, Palacín and Pillay. For this purpose, we will give an alternative presentation of the counterexample as an additive cover of an algebraically closed field. We isolate two fundamental weakenings of the CBP, which already appeared in work of Chatzidakis and Moosa-Pillay and show that they do not hold in the counterexample. In order to do so, a study of imaginaries in additive covers is developed. As a by-product of the presentation, we observe that a pure binding-group-theoretic account of the CBP is unlikely.
“Living Statues” and Nonuments as “Performative Monument Events” in Post-Socialist South-Eastern Europe
Authors: Preda Caterina
Published: June 2022

After the fall of the socialist regimes in South and Eastern Europe socialist statues and monuments were either removed, dislocated, or resignified. Several performance practices have been employed to engage with these statues and monuments. Focusing on the role played by artistic memorialization in the processes of dealing with the communist past, this article uses the concepts of “performative monuments” (Widrich) and “memory events” (Etkind) to analyze several examples of what can be called “performative monuments events.” As many statues were removed, the statues witnessed performative practices in the process of their elimination. The monuments that were conserved were dislocated and exhibited as part of “sculpture park museums” and observed nostalgic, ironic practices of tourists that perform the memory of communism by interacting with the monuments. This article analyzes several examples in Albania, Bulgaria and Romania of socialist monuments that have remained in place or that have been dislocated and resignified by contemporary artists using performative practices of memory events that engage monuments. This exploratory research argues that artists, through their “performative monuments events,” try to bring the people back to replace the statues with “living statues” and to question the absurd megalomaniac monuments using metaphorical, material instruments.
Sedimentary evolution of the Pennsylvanian–Permian Mulargia–Escalaplano molassic basin (Sardinia, Italy): the most complete record in the Southern Variscan Realm
Authors: Costamagna Luca Giacomo
Published: June 2022

Field investigations into the sedimentary evolution of the Mulargia–Escalaplano late- to post-Variscan basin in Sardinia have been carried out. This basin is the only Variscan molassic basin in the Southern Variscan Realm where relationships between all the Late Pennsylvanian to Middle Triassic sedimentary cycles have been detailed for the first time. Here the interplay between tectonics and sedimentation can also be inferred. The investigations evidence that the sedimentary response recorded in the basin matches the progress and final collapse of the southern Variscan chain branch. The basin preserves continental deposits of decreasing energy organized in cycles, separated by weak unconformities related to abrupt volcano-tectonic climaxes and influenced by progressively drying climates. The sedimentary facies and stratigraphy suggest a progressive widening of the basin and decreasing depositional energy to the southeast as well as the migration of the basin depocentre in the same direction. Stratigraphic, environmental and evolutionary correlations between the presently separated NW and SE parts of the basin are reconstructed by considering the role of tectonics. As a result, a detailed lithostratigraphic framework for the Mulargia–Escalaplano basin is proposed. The findings in the sedimentary evolution match the evolution of similar coeval basins of France and Germany, following a pull-apart to extensional model. Based on comparisons with the other Sardinian and SW Europe coeval basins, relationships between ‘limnic’ and red bed successions in SW Europe during the collapse of the Variscan chain are discussed. The renaming of the European tectonomagmatic ‘Mid-Permian Episode’ to the ‘Lower Permian Episode’ is proposed.
TWO MODELS OF INFORMED CONSENT
Authors: Jansen Lynn A.
Published: June 2022

Informed consent is a central concept in the literature on the ethics of clinical care and human subjects research. There is a broad consensus that ethical practice in these domains requires the informed consent of patients and subjects. The requirements of informed consent in these domains, however, are matters of considerable controversy. Some argue that the requirements of informed consent have been inflated, others that they have not been taken seriously enough. This essay argues that both sides are partly right. To advance this argument, the essay distinguishes a general doctrine of informed consent from what it characterizes as “models of informed consent.” A general doctrine articulates a set of requirements for informed consent and then adjusts these requirements to fit the context in which they are to be applied. In contrast, different models of informed consent impose different requirements in different contexts. The essay contends that different models of informed consent are needed for clinical care and clinical research. It outlines these two models, articulates the rationale for distinguishing them, and considers and rebuts the objection that clinical care and clinical research are too deeply intertwined in contemporary medicine for the models approach to apply to them.
WHAT DO EXPERTS KNOW?
Authors: Fileva Iskra
Published: June 2022

Reasonable people agree that whenever possible, we ought to rely on experts to tell us what is true or what the best course of action is. But which experts should we rely on and with regard to what issues? Here, I discuss several dangers that accompany reliance on experts, the most important one of which is this: positions that are offered as expert opinion frequently contain elements outside an expert’s domain of expertise, for instance, values not intrinsic to the given domain. I also talk about the practical implications of accepting my view.
THE VALUE OF JUSTICE-INVOLVED YOUTH: ACCOUNTABILITY THROUGH TECHNOLOGY-DRIVEN POLICIES AND PRACTICES
Authors: Stevens Sally
Published: June 2022

The United States juvenile justice system has primary oversight of youth who come into contact with legal authorities. This system is purposefully distinct from the adult system given the presumption of youths’ reduced culpability for delinquent behavior and increased potential for rehabilitation. Some juvenile court policies and practices are supportive of youth while others may drive youth further into the juvenile justice system. Today, we are at a point in which we can—and should—use information technology to accrue data to unpack the impact of these policies and practices on and across youth. Moreover, technology-driven policies and practices such as electronic monitoring may be detrimental to the well-being of youth, whereas others such as video conferencing could be more widely used to benefit youth. While juvenile courts hold youth accountable for their behavior, courts also need to be accountable to youth by employing data-informed policies and practices that advance the health and well-being of youth.
CULTURAL VALUE AND EVOLVING TECHNOLOGIES: INSTANCES FROM MUSIC AND VISUAL ART
Authors: Asia Daniel , Gordon Robert Edward
Published: June 2022

Scientific advancement is inextricably linked to cultural advancement, and historically the arts have worked hand in hand with technological change. This essay explores some of the connections that exist between science, technology, and the arts, privileging instances where technological change resulted in new forms of artistic creation. Although the role of the arts in contemporary society has ebbed in comparison to that of technology and science, the essay argues that quality, meaningfulness, and longevity are key components in how the arts can retain cultural value in today’s technocentric world. It explores significant instances from the history of music and visual art as the authors make the case that the methods of science and art are both distinct yet commensurate in their ability to shape the values and ideas of contemporary society.
THE PERPETUAL STRUGGLE: HOW THE COEVOLUTION OF HIERARCHY AND RESISTANCE DRIVES THE EVOLUTION OF MORALITY AND INSTITUTIONS
Authors: Buchanan Allen
Published: June 2022

Since the earliest human societies, there has been an ongoing struggle between hierarchy and resistance to hierarchy, and this struggle is a major driver of the evolution of moralities and of institutions. Attempts to initiate or sustain hierarchies are often met with resistance; hierarchs then adopt new strategies, which in turn prompt new strategies of resistance; and so on. The key point is that the struggle is typically conducted using moral concepts in justifications for or against unequal power and involves the stimulation of the moral emotions. Both parties to the struggle treat morality as a valuable strategic resource; and the dynamic of interaction between hierarchs and resisters generates changes in that resource. The hierarch/resister struggle is in part a competition between moral concepts and justifications, and that competition drives the emergence of new moral concepts and justifications, just as competition in other contexts generates innovations. Among the moral concepts generated by the struggle are the following: authority, legitimacy, aristocracy, the divine right of kings, the mandate of heaven, natural rights, civil and political rights, constitutionalism, the rule of law, sovereignty, collective self-determination, exploitation, oppression, and domination.
THE PREHISTORIC ORIGINS OF EUROPEAN ECONOMIC INTEGRATION
Authors: Grantham George
Published: June 2022

It appears likely that at its peak the classical economy was almost as large as that of Western Europe during the Industrial Revolution. The following review of the archeological and document evidence indicates that three events occurring in the first half of the first millennium BC trigger the emergence of a specialized and integrated classical economy after 500 BC: (i) growth in demand for silver as a medium of exchange in economies in the Near East; (ii) technical breakthroughs in hull construction and sailing rig in merchant shipping of the late Bronze Age; (iii) perfection of ferrous metallurgy into the European hinterland. This last event raised agricultural productivity to a level capable of supporting the occupational specialization needed to sustain a vigorous trading economy. To these initial causes may be added the diffusion of alphabetic writing. While it did not create opportunities for long-distance trade, the diffusion of writing supplied the means of responding on a scale large enough economically to matter.
Complex numerals in English: constituents or not?
Authors: HE CHUANSHENG , HER ONE-SOON
Published: June 2022

This article focuses on the controversy over whether complex numerals in English are constituents. Contra the traditional view (e.g. Hurford 1975; Greenberg 1990 [1978]), the cascading structure proposed in Ionin & Matushansky (2006, 2018) maintains that cross-linguistically a complex numeral does not form a constituent to the exclusion of the NP complement. The derivation of an additive complex numeral, e.g.twenty-two people, thus involves an underlying source form with a nominal conjunction, e.g.twenty people and two people. Based on the argumentation established in He (2015) and Heet al.(2017) supporting complex numerals as constituents in Chinese and minority languages in southern China, this article first demonstrates that the non-constituency analysis is not viable for English, as the underlying forms of additive complex numerals may be ill-formed and also not semantically equivalent to the surface forms. We then offer evidence to support the constituency analysis from constituency tests and behavior of post-numeral and pre-numeral modifiers. Finally, we demonstrate that the extra mechanism of grafting, proposed by Meinunger (2015), is unnecessary for English complex numerals.
Use of cannabis in the treatment of animals: a systematic review of randomized clinical trials
Authors: Lima Tácio de Mendonça , Santiago Nathania Rodrigues , Alves Elaine Cristina Ramos , Chaves Douglas Siqueira de Almeida , Visacri Marília Berlofa
Published: June 2022

Cannabis is used in the treatment of several human conditions; however, its use is still less explored in veterinary medicine. This systematic review aims to summarize the evidence of efficacy and safety of the use of cannabis for the treatment of animal disease. A literature search was performed for studies published until 16 March 2021 in five databases. Randomized clinical trials (RCTs) that reported the efficacy or safety of cannabis in the treatment of animal disease were included. The RoB 2 Tool was used to assess the risk of bias. A total of 2427 records were identified, of which six studies fully met the eligibility criteria. RCTs were conducted in dogs with osteoarthritis (n= 4), with epilepsy (n= 1), and with behavioral disorders (n= 1). All studies used cannabidiol (CBD) oil in monotherapy or in combination with other drugs. Studies used CBD at 2 or 2.5 mg kg−1twice daily (n= 4), orally (n= 5), during 4 or 6 weeks (n= 3), and compared CBD with placebo (n= 5). CBD significantly reduced pain and increased activity in dogs with osteoarthritis (n= 3). Moreover, CBD significantly reduced the frequency of seizures in dogs with epilepsy (n= 1) and the aggressive behavior of dogs (n= 1). Although promising results were identified, studies were heterogeneous and presented risks of bias that required caution in the interpretation of findings. Therefore, there was some evidence to support the use of CBD in dogs with osteoarthritis to reduce pain and increased activity, but limited evidence against epilepsy and behavioral problems. In addition, CBD was well tolerated with mild adverse effects. More RCTs with high quality of evidence are needed, including greater numbers of animal subjects, additional species, and clear readout measures to confirm these findings.
Low Thermal Conductivity of Hydrous Phase D Leads to a Self‐Preservation Effect Within a Subducting Slab
Authors: Hsieh Wen‐Pin , Marzotto Enrico , Ishii Takayuki , Dubrovinsky Leonid , Aslandukova Alena A. , Criniti Giacomo , Tsao Yi‐Chi , Lin Chun‐Hung , Tsuchiya Jun , Ohtani Eiji
Published: June 2022

Earth's deep water cycle impacts the physical and chemical properties and geodynamics in its deep interior. However, how dense hydrous magnesium silicates (DHMSs) influence the thermal evolution and dynamics of sinking slabs remains poorly understood. We have precisely measured thermal conductivity of phase D, an important DHMS that could carry large amounts of water from the mantle transition zone to the lower mantle, at high pressure‐temperature conditions. The thermal conductivity of (Al,Fe)‐bearing phase D is lower than those of the pyrolitic mantle and basaltic crust along slab subduction, except for the depth range of ∼800–1,100 km where a spin transition of iron occurs. Numerical simulations indicate that although the spin transition in phase D has minor effects on slab's temperature due to its small volume fraction, the poorly thermally‐conductive hydrous minerals contribute to maintain a cold hydrous layer within a sinking slab, stabilizing slab hydrous minerals and promoting water transportation to the deeper mantle.
Magnetic force microscopy of an operational spin nano-oscillator
Authors: Banuazizi Seyed Amir Hossein , Houshang Afshin , Awad Ahmad A. , Mohammadi Javad , Åkerman Johan , Belova Liubov M.
Published: June 2022

Magnetic force microscopy (MFM) is a powerful technique for studying magnetic microstructures and nanostructures that relies on force detection by a cantilever with a magnetic tip. The detected magnetic tip interactions are used to reconstruct the magnetic structure of the sample surface. Here, we demonstrate a new method using MFM for probing the spatial profile of an operational nanoscale spintronic device, the spin Hall nano-oscillator (SHNO), which generates high-intensity spin wave auto-oscillations enabling novel microwave applications in magnonics and neuromorphic computing. We developed an MFM system by adding a microwave probe station to allow electrical and microwave characterization up to 40 GHz during the MFM process. SHNOs—based on NiFe/Pt bilayers with a specific design compatible with the developed system—were fabricated and scanned using a Co magnetic force microscopy tip with 10 nm spatial MFM resolution, while a DC current sufficient to induce auto-oscillation flowed. Our results show that this developed method provides a promising path for the characterization and nanoscale magnetic field imaging of operational nano-oscillators.
CA125: a novel cardiac biomarker for infants with congenital diaphragmatic hernia
Authors: Schroeder Lukas , Pugnaloni Flaminia , Dolscheid-Pommerich Ramona , Geipel Annegret , Berg Christoph , Holdenrieder Stefan , Mueller Andreas , Kipfmueller Florian
Published: June 2022

Background

The carbohydrate antigen 125 (CA125) was proven as a robust biomarker for risk stratification in adults with heart failure. This is the first study analyzing CA125 in a cohort of infants with congenital diaphragmatic hernia (CDH).

Methods

Sixty-eight infants with CDH, treated at the University Children’s Hospital Bonn (Germany), between January 2018 and February 2021, were prospectively enrolled for analysis. CA125 values were measured at the following timepoints: 6,12, 24, 48 h, and during ECMO daily from day 1 to day 7.

Results

In infants not surviving to discharge, CA125 values were significantly higher at day 1 (6, 12, and 24 h). Infants with subsequent need for ECMO presented significantly higher CA125 values at 12 h of life. During ECMO, CA125 values measured at day 1 were significantly higher in infants not surviving to discharge. In the ROC analysis, a CA125 value of ≥10 U/ml was calculated as optimal cut-off for the prediction of ECMO and in-hospital mortality. CA125 values correlated significantly with the severity of PH and ventricular dysfunction.

Conclusions

CA125 values correlate significantly with echocardiographic markers of PH and ventricular dysfunction and correlate significantly with parameters of disease severity (need for ECMO, mortality).

Impact

CA125 was proven as robust cardiac biomarker in adult cohorts.

Information about the utility as a biomarker in neonatal cohorts is lacking.

This is the first study analyzing CA125 as a cardiac biomarker in a cohort of infants with congenital diaphragmatic hernia (CDH).

CA125 correlates significantly with markers of echocardiographic assessment (PH and ventricular dysfunction) in infants with CDH and helps to identify infants at high risk for ECMO and in-hospital mortality.

The results underline the need for the inclusion of cardiac biomarkers in the clinical routine in neonates at risk for cardiopulmonary failure.
Genetics of autism spectrum disorder: an umbrella review of systematic reviews and meta-analyses
Authors: Qiu Shuang , Qiu Yingjia , Li Yan , Cong Xianling
Published: June 2022

Autism spectrum disorder (ASD) is a class of neurodevelopmental conditions with a large epidemiological and societal impact worldwide. To date, numerous studies have investigated the associations between genetic variants and ASD risk. To provide a robust synthesis of published evidence of candidate gene studies for ASD, we performed an umbrella review (UR) of meta-analyses of genetic studies for ASD (PROSPERO registration number: CRD42021221868). We systematically searched eight English and Chinese databases from inception to March 31, 2022. Reviewing of eligibility, data extraction, and quality assessment were performed by two authors. In total, 28 of 5062 retrieved articles were analyzed, which investigated a combined 41 single nucleotide polymorphisms (SNPs) of nine candidate genes. Overall, 12 significant SNPs of CNTNAP2, MTHFR, OXTR, SLC25A12, and VDR were identified, of which associations with suggestive evidence included the C677T polymorphism of MTHFR (under allelic, dominant, and heterozygote models) and the rs731236 polymorphism of VDR (under allelic and homozygote models). Associations with weak evidence included the rs2710102 polymorphism of CNTNAP2 (under allelic, homozygote, and recessive models), the rs7794745 polymorphism of CNTNAP2 (under dominant and heterozygote models), the C677T polymorphism of MTHFR (under homozygote model), and the rs731236 polymorphism of VDR (under dominant and recessive models). Our UR summarizes research evidence on the genetics of ASD and provides a broad and detailed overview of risk genes for ASD. The rs2710102 and rs7794745 polymorphisms of CNTNAP2, C677T polymorphism of MTHFR, and rs731236 polymorphism of VDR may confer ASD risks. This study will provide clinicians and healthcare decision-makers with evidence-based information about the most salient candidate genes relevant to ASD and recommendations for future treatment, prevention, and research.
ID proteins promote the survival and primed-to-naive transition of human embryonic stem cells through TCF3-mediated transcription
Authors: Jiang Haibin , Du Mingxia , Li Yaning , Zhou Tengfei , Lei Jia , Liang Hongqing , Zhong Zhen , Al-Lamki Rafia S. , Jiang Ming , Yang Jun
Published: June 2022

Inhibition of DNA binding proteins 1 and 3 (ID1 and ID3) are important downstream targets of BMP signalling that are necessary for embryonic development. However, their specific roles in regulating the pluripotency of human embryonic stem cells (hESCs) remain unclear. Here, we examined the roles of ID1 and ID3 in primed and naive-like hESCs and showed that ID1 and ID3 knockout lines (IDs KO) exhibited decreased survival in both primed and naive-like state. IDs KO lines in the primed state also tended to undergo pluripotent dissolution and ectodermal differentiation. IDs KO impeded the primed-to-naive transition (PNT) of hESCs, and overexpression of ID1 in primed hESCs promoted PNT. Furthermore, single-cell RNA sequencing demonstrated that ID1 and ID3 regulated the survival and pluripotency of hESCs through the AKT signalling pathway. Finally, we showed that TCF3 mediated transcriptional inhibition of MCL1 promotes AKT phosphorylation, which was confirmed by TCF3 knockdown in KO lines. Our study suggests that IDs/TCF3 acts through AKT signalling to promote survival and maintain pluripotency of both primed and naive-like hESCs.
Cysteamine exerts in vitro antiviral activity against the SARS-CoV-2 Delta and Omicron variants
Authors: Alonzi Tonino , Aiello Alessandra , Repele Federica , Falasca Laura , Francalancia Massimo , Garbuglia Anna Rosa , Delogu Giovanni , Nicastri Emanuele , Piacentini Mauro , Goletti Delia
Published: June 2022

The novel SARS-CoV-2 variants of concern (VOC) represent a considerable global alarm because their mutations are known to affect transmissibility and cause immune escape. While preventing severe disease and deaths, the available vaccines do not avoid infection; therefore, COVID-19 disease management still requires effective therapies. We have recently reported that the aminothiol cysteamine, a drug already applied to humans, exerts direct antiviral activity against SARS-CoV-2 and has in vitro immunomodulatory effect. To evaluate whether this compound exerts antiviral effects also against SARS-CoV-2 variants, we performed different infected cell-based assays using Wild type, Delta, or Omicron VOC. We found that cysteamine significantly reduces the cytopathic effect induced by SARS-CoV-2 Wild type strain and Delta variant in Vero E6 cells. On the other hand, cysteamine had no effects on the survival of cells infected with the Omicron variant, due to the lack of cytotoxicity on Vero E6 cells, at least when infected at MOI = 0.001 for 72 h. Moreover, cysteamine significantly reduced the production of Wild type, Delta, and Omicron variants as measured by the virus released in the culture media (Vero E6 and Calu-3 cells) and by transmission electron microscopy analysis (Vero E6 cells). Notably, cysteamine is more effective in inhibiting the Omicron rather than Delta or Wild type viruses, with an 80% inhibition of Omicron production compared to 40% of Wild type and Delta variant. Overall, our findings demonstrate that cysteamine exerts direct antiviral actions against SARS-CoV-2 Delta and Omicron variants, in addition to the Wild type virus. Our data further demonstrate that cysteamine is a good candidate as repurposing drug for the treatment of SARS-CoV-2 infection for the present and, likely, the future VOC and, therefore, it would be important to investigate its clinical relevance in randomized clinical trials.
Remote solid cancers rewire hepatic nitrogen metabolism via host nicotinamide-N-methyltransferase
Authors: Mizuno Rin , Hojo Hiroaki , Takahashi Masatomo , Kashio Soshiro , Enya Sora , Nakao Motonao , Konishi Riyo , Yoda Mayuko , Harata Ayano , Hamanishi Junzo , Kawamoto Hiroshi , Mandai Masaki , Suzuki Yutaka , Miura Masayuki , Bamba Takeshi , Izumi Yoshihiro , Kawaoka Shinpei
Published: June 2022

Cancers disrupt host homeostasis in various manners but the identity of host factors underlying such disruption remains largely unknown. Here we show that nicotinamide-N-methyltransferase (NNMT) is a host factor that mediates metabolic dysfunction in the livers of cancer-bearing mice. Multiple solid cancers distantly increase expression of Nnmt and its product 1-methylnicotinamide (MNAM) in the liver. Multi-omics analyses reveal suppression of the urea cycle accompanied by accumulation of amino acids, and enhancement of uracil biogenesis in the livers of cancer-bearing mice. Importantly, genetic deletion of Nnmt leads to alleviation of these metabolic abnormalities, and buffers cancer-dependent weight loss and reduction of the voluntary wheel-running activity. Our data also demonstrate that MNAM is capable of affecting urea cycle metabolites in the liver. These results suggest that cancers up-regulate the hepatic NNMT pathway to rewire liver metabolism towards uracil biogenesis rather than nitrogen disposal via the urea cycle, thereby disrupting host homeostasis.
Challenging the highstand-dormant paradigm for land-detached submarine canyons
Authors: Heijnen M. S. , Mienis F. , Gates A. R. , Bett B. J. , Hall R. A. , Hunt J. , Kane I. A. , Pebody C. , Huvenne V. A. I. , Soutter E. L. , Clare M. A.
Published: June 2022

Sediment, nutrients, organic carbon and pollutants are funnelled down submarine canyons from continental shelves by sediment-laden flows called turbidity currents, which dominate particulate transfer to the deep sea. Post-glacial sea-level rise disconnected more than three quarters of the >9000 submarine canyons worldwide from their former river or long-shore drift sediment inputs. Existing models therefore assume that land-detached submarine canyons are dormant in the present-day; however, monitoring has focused on land-attached canyons and this paradigm remains untested. Here we present the most detailed field measurements yet of turbidity currents within a land-detached submarine canyon, documenting a remarkably similar frequency (6 yr−1) and speed (up to 5–8 ms−1) to those in large land-attached submarine canyons. Major triggers such as storms or earthquakes are not required; instead, seasonal variations in cross-shelf sediment transport explain temporal-clustering of flows, and why the storm season is surprisingly absent of turbidity currents. As >1000 other canyons have a similar configuration, we propose that contemporary deep-sea particulate transport via such land-detached canyons may have been dramatically under-estimated.
Deciphering the phase transition-induced ultrahigh piezoresponse in (K,Na)NbO3-based piezoceramics
Authors: Zhang Mao-Hua , Shen Chen , Zhao Changhao , Dai Mian , Yao Fang-Zhou , Wu Bo , Ma Jian , Nan Hu , Wang Dawei , Yuan Qibin , da Silva Lucas Lemos , Fulanović Lovro , Schökel Alexander , Liu Peitao , Zhang Hongbin , Li Jing-Feng , Zhang Nan , Wang Ke , Rödel Jürgen , Hinterstein Manuel
Published: June 2022

Here, we introduce phase change mechanisms in lead-free piezoceramics as a strategy to utilize attendant volume change for harvesting large electrostrain. In the newly developed (K,Na)NbO3 solid-solution at the polymorphic phase boundary we combine atomic mapping of the local polar vector with in situ synchrotron X-ray diffraction and density functional theory to uncover the phase change and interpret its underlying nature. We demonstrate that an electric field-induced phase transition between orthorhombic and tetragonal phases triggers a dramatic volume change and contributes to a huge effective piezoelectric coefficient of 1250 pm V−1 along specific crystallographic directions. The existence of the phase transition is validated by a significant volume change evidenced by the simultaneous recording of macroscopic longitudinal and transverse strain. The principle of using phase transition to promote electrostrain provides broader design flexibility in the development of high-performance piezoelectric materials and opens the door for the discovery of high-performance future functional oxides.
Organic donor-acceptor heterojunctions for high performance circularly polarized light detection
Authors: Zhu Danlei , Jiang Wei , Ma Zetong , Feng Jiajing , Zhan Xiuqin , Lu Cheng , Liu Jie , Liu Jie , Hu Yuanyuan , Wang Dong , Zhao Yong Sheng , Wang Jianpu , Wang Zhaohui , Jiang Lang
Published: June 2022

Development of highly efficient and stable lateral organic circularly polarized light photodetector is a fundamental prerequisite for realization of circularly polarized light integrated applications. However, chiral semiconductors with helical structure are usually found with intrinsically low field-effect mobilities, which becomes a bottleneck for high-performance and multi-wavelength circularly polarized light detection. To address this problem, here we demonstrate a novel strategy to fabricate multi-wavelength circularly polarized light photodetector based on the donor-acceptor heterojunction, where efficient exciton separation enables chiral acceptor layer to provide differentiated concentration of holes to the channel of organic field-effect transistors. Benefitting from the low defect density at the semiconductor/dielectric interface, the photodetectors exhibit excellent stability, enabling current roll-off of about 3–4% over 500 cycles. The photocurrent dissymmetry value and responsivity for circularly polarized light photodetector in air are 0.24 and 0.28 A W−1, respectively. We further demonstrate circularly polarized light communication based on a real-time circularly polarized light detector by decoding the light signal. As the proof-of-concept, the results hold the promise of large-scale circularly polarized light integrated photonic applications.
Slowing quantum decoherence of oscillators by hybrid processing
Authors: Park Kimin , Hastrup Jacob , Neergaard-Nielsen Jonas Schou , Brask Jonatan Bohr , Filip Radim , Andersen Ulrik L.
Published: June 2022

Quantum information encoded into the superposition of coherent states is an illustrative representation of practical applications of macroscopic quantum coherence possessing. However, these states are very sensitive to energy loss, losing their non-classical aspects of coherence very rapidly. An available deterministic strategy to slow down this decoherence process is to apply a Gaussian squeezing transformation prior to the loss as a protective step. Here, we propose a deterministic hybrid protection scheme utilizing strong but feasible interactions with two-level ancillas immune to spontaneous emission. We verify the robustness of the scheme against the dephasing of qubit ancilla. Our scheme is applicable to complex superpositions of coherent states in many oscillators, and remarkably, the robustness to loss is enhanced with the amplitude of the coherent states. This scheme can be realized in experiments with atoms, solid-state systems, and superconducting circuits.
Optimization of BRET saturation assays for robust and sensitive cytosolic protein–protein interaction studies
Authors: Besson Benoit , Eun Hyeju , Kim Seonhee , Windisch Marc P. , Bourhy Herve , Grailhe Regis
Published: June 2022

Bioluminescence resonance energy transfer (BRET) saturation is a method of studying protein–protein interaction (PPI) upon quantification of the dependence of the BRET signal on the acceptor/donor (A:D) expression ratio. In this study, using the very bright Nluc/YFP BRET pair acquired respectively with microplate reader and automated confocal microscopy, we significantly improved BRET saturation assay by extending A:D expression detection range and normalizing A:D expression with a new BRET-free probe. We next found that upon using variable instead of fixed amount of donor molecules co-expressed with increasing acceptor concentrations, BRET saturation assay robustness can be further improved when studying cytosolic protein, although the relative amounts of dimers (BRETmax) and the relative dimer affinity (BRET50) remain similar. Altogether, we show that our method can be applied to many PPI networks, involving the NF-κB pathway, high-affinity nanobody, rabies virus-host interactions, mTOR complex and JAK/STAT signaling. Altogether our approach paves the way for robust PPI validation and characterization in living cells.
Comparison of the gut microbiome of sacbrood virus-resistant and -susceptible Apis cerana from South Korea
Authors: Yun Bo-Ram , Truong A-Tai , Choi Yong Soo , Lee Man Young , Kim Byoung Yong , Seo Minjung , Yoon Soon-Seek , Yoo Mi-Sun , Van Quyen Dong , Cho Yun Sang
Published: June 2022

Honey bees are important pollinators for the conservation of the ecosystem and agricultural products and provide a variety of products important for human use, such as honey, pollen, and royal jelly. Sacbrood disease (SD) is a devastating viral disease in Apis cerana; an effective preventive measure for SD is urgently needed. In this study, the relationship between the gut microbiome of honey bees and SD was investigated by pyrosequencing. Results revealed that sacbrood virus (SBV)-resistant A. cerana strains harbour a unique acetic acid bacterium, Bombella intestini, and the lactic acid bacteria (LAB) Lactobacillus (unclassified)_uc, Bifidobacterium longum, B. catenulatum, Lactococcus lactis, and Leuconostoc mesenteroides in larvae and Hafnia alvei, B. indicum, and the LAB L. mellifer and Lactobacillus HM215046_s in adult bees. Changes in the gut microbiome due to SBV infection resulted in loss of bacteria that could affect host nutrients and inhibit honey bee pathogens, such as Gilliamella JFON_s, Gilliamella_uc, Pseudomonas putida, and L. kunkeei in A. cerana larvae and Frischella_uc, Pantoea agglomerans, Snodgrassella_uc, and B. asteroides in adult bees. These findings provide important information for the selection of probiotics for A. cerana larvae and adults to prevent pathogenic infections and keep honey bees healthy.
A new method to quantify left ventricular mass by 2D echocardiography
Authors: Kristensen Charlotte Burup , Myhr Katrine Aagaard , Grund Frederik Fasth , Vejlstrup Niels , Hassager Christian , Mattu Raj , Mogelvang Rasmus
Published: June 2022

Increased left ventricular mass (LVM) is a strong independent predictor for adverse cardiovascular events, but conventional echocardiographic methods are limited by poor reproducibility and accuracy. We developed a novel method based on adding the mean wall thickness from the parasternal short axis view, to the left ventricular end-diastolic volume acquired using the biplane model of discs. The participants (n = 85) had various left ventricular geometries and were assessed using echocardiography followed immediately by cardiac magnetic resonance, as reference. We compared our novel two-dimensional (2D) method to various conventional one-dimensional (1D) and other 2D methods as well as the three-dimensional (3D) method. Our novel method had better reproducibility in intra-examiner [coefficients of variation (CV) 9% vs. 11–14%] and inter-examiner analysis (CV 9% vs. 10–20%). Accuracy was similar to the 3D method (mean difference ± 95% limits of agreement, CV): Novel: 2 ± 50 g, 15% vs. 3D: 2 ± 51 g, 16%; and better than the “linear” 1D method by Devereux (7 ± 76 g, 23%). Our novel method is simple, has considerable better reproducibility and accuracy than conventional “linear” 1D methods, and similar accuracy as the 3D-method. As the biplane model forms part of the standard echocardiographic protocol, it does not require specific training and provides a supplement to the modern echocardiographic report.
Screening and characterisation of proteins interacting with the mitogen-activated protein kinase Crmapk in the fungus Clonostachys chloroleuca
Authors: Lv Binna , Fan Lele , Li Shidong , Sun Manhong
Published: June 2022

Clonostachys chloroleuca 67-1 (formerly C. rosea 67-1) is a promising mycoparasite with great potential for controlling various plant fungal diseases. The mitogen-activated protein kinase (MAPK)-encoding gene Crmapk is of great importance to the mycoparasitism and biocontrol activities of C. chloroleuca. To investigate the molecular mechanisms underlying the role of Crmapk in mycoparasitism, a high-quality yeast two hybrid (Y2H) library of C. chloroleuca 67-1 was constructed, and proteins interacting with Crmapk were characterised. The library contained 1.6 × 107 independent clones with a recombination rate of 96%, and most inserted fragments were > 1 kb. The pGBKT7-Crmapk bait vector with no self-activation or toxicity to yeast cells was used to screen interacting proteins from the Y2H library, resulting in 60 candidates, many linked to metabolism, cellular processes and signal transduction. Combined bioinformatics and transcriptome analyses of C. chloroleuca 67-1 and ΔCrmapk mutant mycoparasitising Sclerotinia sclerotiorum sclerotia, 41 differentially expressed genes were identified, which might be the targets of the Fus3/Kss1-MAPK pathway. The results provide a profile of potential protein interactions associated with MAPK enzymes in mycoparasites, and are of great significance for understanding the mechanisms of Crmapk regulating C. chloroleuca mycoparasitism.
Newly synthesized chitosan-nanoparticles attenuate carbendazim hepatorenal toxicity in rats via activation of Nrf2/HO1 signalling pathway
Authors: Mo Elshazly , Ebedy Yasmin A. , Ibrahim Marwa A. , Farroh Khaled Y. , Hassanen Eman I.
Published: June 2022

Widespread application of carbendazim (CBZ) is a major environmental impact because of its residues that caused multi-organ dysfunction. Recently, Chitosan nanoparticles (CS-NPs) are extensively used as nanocarriers due to their non-toxic and biodegradable nature. Therefore, the current study aimed to investigate the possible mechanistic pathway of modified CS-NPs to reduce the hepatic and nephrotoxicity of CBZ in rats. CS-NPs were synthesized by the ionic gelation method by using ascorbic acid instead of acetic acid to increase its antioxidant efficiency. Twenty-adult male Wistar rats were grouped (n = 5) as follows: Group (1) negative control, group (2) received CS-NPs, group (3) received CBZ, and group (4) co-administered CS-NPs with CBZ. Rats received the aforementioned materials daily by oral gavage for 28 days and weighed weekly. The results revealed that CBZ receiving group showed severe histopathological alterations in the liver and kidney sections including cellular necrosis and interstitial inflammation confirmed by immunostaining and showed marked immunopositivity of iNOS and caspase-3 protein. There were marked elevations in the serum levels of ALT, AST, urea, and creatinine with a significant increase in MDA levels and decrease in TAC levels. Upregulation of the Keap1 gene and down-regulation of Nrf2 and HO-1 genes were also observed. Co-treatment of rats by CS-NPs with CBZ markedly improved all the above-mentioned toxicological parameters and return liver and kidney tissues to normal histological architecture. We concluded that CBZ caused hepatorenal toxicity via oxidative stress and the Nrf2/HO-1 pathway and CS-NPs could reduce CBZ toxicity via their antioxidant, anti-apoptotic, and anti-inflammatory effects.
A prolonged innate systemic immune response in COVID-19
Authors: Ekstedt Sandra , Piersiala Krzysztof , Petro Marianne , Karlsson Agneta , Kågedal Åsa , Kumlien Georén Susanna , Cardell Lars Olaf
Published: June 2022

Despite the introduction of vaccines, COVID-19 still affects millions of people worldwide. A better understanding of pathophysiology and the discovery of novel therapies are needed. One of the cells of interest in COVID-19 is the neutrophil. This cell type is being recruited to a site of inflammation as one of the first immune cells. In this project, we investigated a variety of neutrophils phenotypes during COVID-19 by measuring the expression of markers for migration, maturity, activation, gelatinase granules and secondary granules using flow cytometry. We show that neutrophils during COVID-19 exhibit altered phenotypes compared to healthy individuals. The activation level including NETs production and maturity of neutrophils seem to last longer during COVID-19 than expected for innate immunity. Neutrophils as one of the drivers of severe cases of COVID-19 are considered as potential treatment targets. However, for a successful implementation of treatment, there is a need for a better understanding of neutrophil functions and phenotypes in COVID-19. Our study answers some of those questions.
Vectorcardiography-derived index allows a robust quantification of ventricular electrical synchrony
Authors: Fernández Juan M. F. , Spagnuolo Damián N. , Politi María T. , Tello Santacruz Iván A. , Schiavone Miguel , Cáceres Monié César , Avaca Horacio A. , Chara Osvaldo
Published: June 2022

Alteration of muscle activation sequence is a key mechanism in heart failure with reduced ejection fraction. Successful cardiac resynchronization therapy (CRT), which has become standard therapy in these patients, is limited by the lack of precise dyssynchrony quantification. We implemented a computational pipeline that allows assessment of ventricular dyssynchrony by vectorcardiogram reconstruction from the patient’s electrocardiogram. We defined a ventricular dyssynchrony index as the distance between the voltage and speed time integrals of an individual observation and the linear fit of these variables obtained from a healthy population. The pipeline was tested in a 1914-patient population. The dyssynchrony index showed minimum values in heathy controls and maximum values in patients with left bundle branch block (LBBB) or with a pacemaker (PM). We established a critical dyssynchrony index value that discriminates electrical dyssynchronous patterns (LBBB and PM) from ventricular synchrony. In 10 patients with PM or CRT devices, dyssynchrony indexes above the critical value were associated with high time to peak strain standard deviation, an echocardiographic measure of mechanical dyssynchrony. Our index proves to be a promising tool to evaluate ventricular activation dyssynchrony, potentially enhancing the selection of candidates for CRT, device configuration during implantation, and post-implant optimization.
Composition and decomposition of rhizoma peanut (Arachis glabrata Benth.) belowground biomass
Authors: S. Santos Erick R. , B. Dubeux José C. , Sollenberger Lynn E. , B. Siqueira Michelle C. , O. S. van Cleef Flávia , M. Jaramillo David , Q. S. D. Zagato Luana , D. Queiroz Luana M. , Garcia Liza , V. Garcia Carlos C. , Ruiz-Moreno Martin
Published: June 2022

Roots and rhizomes can play an important role in nutrient cycling, however, few studies have investigated how their decomposition pattern is affected by defoliation and time of the year. This 2-year study evaluated root-rhizome composition and decomposition of a warm-season rhizomatous perennial legume [rhizoma peanut (RP; Arachis glabrata Benth.)] under continuous stocking or when defoliated by clipping every 56 days. A 168-days incubation trial was performed to determine disappearance of biomass and N and changes in acid detergent fiber (ADF), acid detergent insoluble N (ADIN), and C:N ratio. Additionally, three 56-days incubations were performed each year to evaluate the disappearance coefficient (B0) and relative decay rate (k). There were no treatment differences in any response for the 168-days incubation. After 168 days, 21 and 60% of initial biomass and initial N remained, respectively. Relative decay rate for OM and N were 0.0088 and 0.0035 g g−1 day−1, respectively. Carbon-to-N ratio decreased from 29 at day 0 to 17 at day 168. Concentration of ADIN increased from 6.9 to 19.3 g kg−1, plateauing at day 79. The B0 and k for remaining OM and N were greater in late than early season and could be explained by greater N concentration and lesser C:N ratio. Rapid decomposition, difference in C:N ratio from day 0 to 168, and the increase in ADIN concentration during incubation indicate large amounts of root-rhizome-soluble C at initiation of incubation. These data indicate that RP root-rhizome turnover is more responsive to season than defoliation frequency.