bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Residence Area and Basic Livelihood Conditions on the Prevalence and Diagnosis Experience of Osteoporosis in Postmenopausal Women Aged over 50 Years: Evaluation Using Korea National Health and Nutrition Examination Survey Data
Authors: Kang Suk-Woong , Yang Ji-Hee , Shin Won-Chul , Kim Yoon-Ji , Choi Min-Hyeok
Published: September 2021

Patients with osteoporosis are asymptomatic and are at risk for fractures. Therefore, early detection and interventions are important. We found that a population with a low socioeconomic status living in rural areas was reported to have a high osteoporosis prevalence but a relatively low diagnosis rate. Research on the disparity of osteoporosis prevalence and treatment from the socioeconomic perspective was conducted. This study aimed to investigate the influence of residence area and basic livelihood conditions on osteoporosis prevalence and diagnosis in postmenopausal women aged over 50 years. The cross-sectional data of 1477 postmenopausal women aged over 50 years obtained from the Korea National Health and Nutrition Examination Survey V-2 were analyzed. Univariate analyses were performed to calculate the prevalence and diagnosis rate according to risk factor categories. A multivariate logistic regression analysis was performed to identify the influence of residence area and basic livelihood conditions after controlling for other factors. The osteoporosis prevalence in basic livelihood beneficiaries (53.7%) and rural area residents (41.9%) was higher than that in non-beneficiaries (33.1%) and urban area residents (31.8%). There was no significant difference in the diagnosis rates in relation to the basic livelihood conditions or residence areas. The adjusted odds ratio for the prevalence among the beneficiaries living in rural areas was 2.08 (95% confidence interval: 1.06–4.10). However, the odds ratio for diagnosis was not significantly different. Earlier screening examination policies for osteoporosis in postmenopausal women with a low socioeconomic status living in rural areas are needed.
And Yet It Moves: Oxidation of the Nuclear Autoantigen La/SS-B Is the Driving Force for Nucleo-Cytoplasmic Shuttling
Authors: Berndt Nicole , Bippes Claudia C. , Michalk Irene , Bartsch Tabea , Arndt Claudia , Puentes-Cala Edinson , Soto Javier Andrés , Loureiro Liliana R. , Kegler Alexandra , Bachmann Dominik , Gross Joanne K. , Gross Tim , Kurien Biji T. , Scofield R. Hal , Farris A. Darise , James Judith A. , Bergmann Ralf , Schmitz Marc , Feldmann Anja , Bachmann Michael P.
Published: September 2021

Decades ago, we and many other groups showed a nucleo-cytoplasmic translocation of La protein in cultured cells. This shuttling of La protein was seen after UV irradiation, virus infections, hydrogen peroxide exposure and the Fenton reaction based on iron or copper ions. All of these conditions are somehow related to oxidative stress. Unfortunately, these harsh conditions could also cause an artificial release of La protein. Even until today, the shuttling and the cytoplasmic function of La/SS-B is controversially discussed. Moreover, the driving mechanism for the shuttling of La protein remains unclear. Recently, we showed that La protein undergoes redox-dependent conformational changes. Moreover, we developed anti-La monoclonal antibodies (anti-La mAbs), which are specific for either the reduced form of La protein or the oxidized form. Using these tools, here we show that redox-dependent conformational changes are the driving force for the shuttling of La protein. Moreover, we show that translocation of La protein to the cytoplasm can be triggered in a ligand/receptor-dependent manner under physiological conditions. We show that ligands of toll-like receptors lead to a redox-dependent shuttling of La protein. The shuttling of La protein depends on the redox status of the respective cell type. Endothelial cells are usually resistant to the shuttling of La protein, while dendritic cells are highly sensitive. However, the deprivation of intracellular reducing agents in endothelial cells makes endothelial cells sensitive to a redox-dependent shuttling of La protein.
Decapitation Experiments Combined with the Transcriptome Analysis Reveal the Mechanism of High Temperature on Chrysanthemum Axillary Bud Formation
Authors: Yang Yujie , Ahmad Sagheer , Yang Qingqing , Yuan Cunquan , Zhang Qixiang
Published: September 2021

Temperature is an important factor that largely affects the patterns of shoot branching in plants. However, the effect and mechanism of temperature on axillary bud development in chrysanthemum remains poorly defined. The purpose of the present study is to investigate the effect of high temperature on the axillary bud growth and the mechanism of axillary bud formation in chrysanthemum. Decapitation experiments combined with the transcriptome analysis were designed. Results showed that the axillary bud length was significantly inhibited by high temperature. Decapitation of primary shoot (primary decapitation) resulted in slower growth of axillary buds (secondary buds) under 35 °C. However, secondary decapitation resulted in complete arrest of tertiary buds at high temperature. These results demonstrated that high temperature not only inhibited axillary bud formation but also retarded bud outgrowth in chrysanthemum. Comparative transcriptome suggested differentially expressed gene sets and identified important modules associated with bud formation. This research helped to elucidate the regulatory mechanism of high temperature on axillary bud growth, especially bud formation in chrysanthemum. Meanwhile, in-depth studies of this imperative temperature signaling can offer the likelihood of vital future applications in chrysanthemum breeding and branching control.
The First Cytoplasmic Loop in the Core Structure of the ABCC1 (Multidrug Resistance Protein 1; MRP1) Transporter Contains Multiple Amino Acids Essential for Its Expression
Authors: Conseil Gwenaëlle , Cole Susan P. C.
Published: September 2021

ABCC1 (human multidrug resistance protein 1 (hMRP1)) is an ATP-binding cassette transporter which effluxes xeno- and endobiotic organic anions and confers multidrug resistance through active drug efflux. The 17 transmembrane α-helices of hMRP1 are distributed among three membrane spanning domains (MSD0, 1, 2) with MSD1,2 each followed by a nucleotide binding domain to form the 4-domain core structure. Eight conserved residues in the first cytoplasmic loop (CL4) of MSD1 in the descending α-helix (Gly392, Tyr404, Arg405), the perpendicular coupling helix (Asn412, Arg415, Lys416), and the ascending α-helix (Glu422, Phe434) were targeted for mutagenesis. Mutants with both alanine and same charge substitutions of the coupling helix residues were expressed in HEK cells at wild-type hMRP1 levels and their transport activity was only moderately compromised. In contrast, mutants of the flanking amino acids (G392I, Y404A, R405A/K, E422A/D, and F434Y) were very poorly expressed although Y404F, E422D, and F434A were readily expressed and transport competent. Modeling analyses indicated that Glu422 and Arg615 could form an ion pair that might stabilize transporter expression. However, this was not supported by exchange mutations E422R/R615E which failed to improve hMRP1 levels. Additional structures accompanied by rigorous biochemical validations are needed to better understand the bonding interactions crucial for stable hMRP1 expression.
Defective Efferocytosis in a Murine Model of Sjögren’s Syndrome Is Mediated by Dysfunctional Mer Tyrosine Kinase Receptor
Authors: Witas Richard , Rasmussen Astrid , Scofield Robert H. , Radfar Lida , Stone Donald U. , Grundahl Kiely , Lewis David , Sivils Kathy L. , Lessard Christopher J. , Farris A. Darise , Nguyen Cuong Q.
Published: September 2021

Sjögren’s syndrome (SjS) is a chronic autoimmune disease primarily involving the exocrine glands in which the involvement of the innate immune system is largely uncharacterized. Mer signaling has been found to be protective in several autoimmune diseases but remains unstudied in SjS. Here, we investigated the role of Mer signaling in SjS. Mer knockout (MerKO) mice were examined for SjS disease criteria. SjS-susceptible (SjSS) C57BL/6.NOD-Aec1Aec2 mice were assessed for defective Mer signaling outcomes, soluble Mer (sMer) levels, A disintegrin and metalloprotease 17 (ADAM17) activity, and Rac1 activation. In addition, SjS patient plasma samples were evaluated for sMer levels via ELISA, and sMer levels were correlated to disease manifestations. MerKO mice developed submandibular gland (SMG) lymphocytic infiltrates, SMG apoptotic cells, anti-nuclear autoantibodies (ANA), and reduced saliva flow. Mer signaling outcomes were observed to be diminished in SjSS mice, as evidenced by reduced Rac1 activation in SjSS mice macrophages in response to apoptotic cells and impaired efferocytosis. Increased sMer was also detected in SjSS mouse sera, coinciding with higher ADAM17 activity, the enzyme responsible for cleavage and inactivation of Mer. sMer levels were elevated in patient plasma and positively correlated with focus scores, ocular staining scores, rheumatoid factors, and anti-Ro60 levels. Our data indicate that Mer plays a protective role in SjS, similar to other autoimmune diseases. Furthermore, we suggest a series of events where enhanced ADAM17 activity increases Mer inactivation and depresses Mer signaling, thus removing protection against the loss of self-tolerance and the onset of autoimmune disease in SjSS mice.
Building Personalized Cancer Therapeutics through Multi-Omics Assays and Bacteriophage-Eukaryotic Cell Interactions
Authors: Wang Qing
Published: September 2021

Bacteriophage-eukaryotic cell interaction provides the biological foundation of Phage Display technology, which has been widely adopted in studies involving protein-protein and protein-peptide interactions, and it provides a direct link between the proteins and the DNA encoding them. Phage display has also facilitated the development of new therapeutic agents targeting personalized cancer mutations. Proteins encoded by mutant genes in cancers can be processed and presented on the tumor cell surface by human leukocyte antigen (HLA) molecules, and such mutant peptides are called Neoantigens. Neoantigens are naturally existing tumor markers presented on the cell surface. In clinical settings, the T-cell recognition of neoantigens is the foundation of cancer immunotherapeutics. This year, we utilized phage display to successfully develop the 1st antibody-based neoantigen targeting approach for next-generation personalized cancer therapeutics. In this article, we discussed the strategies for identifying neoantigens, followed by using phage display to create personalized cancer therapeutics—a complete pipeline for personalized cancer treatment.
Impact of Genomic and Transcriptomic Resources on Apiaceae Crop Breeding Strategies
Authors: Palumbo Fabio , Vannozzi Alessandro , Barcaccia Gianni
Published: September 2021

The Apiaceae taxon is one of the most important families of flowering plants and includes thousands of species used for food, flavoring, fragrance, medical and industrial purposes. This study had the specific intent of reviewing the main genomics and transcriptomic data available for this family and their use for the constitution of new varieties. This was achieved starting from the description of the main reproductive systems and barriers, with particular reference to cytoplasmic (CMS) and nuclear (NMS) male sterility. We found that CMS and NMS systems have been discovered and successfully exploited for the development of varieties only in Foeniculum vulgare, Daucus carota, Apium graveolens and Pastinaca sativa; whereas, strategies to limit self-pollination have been poorly considered. Since the constitution of new varieties benefits from the synergistic use of marker-assisted breeding in combination with conventional breeding schemes, we also analyzed and discussed the available SNP and SSR marker datasets (20 species) and genomes (8 species). Furthermore, the RNA-seq studies aimed at elucidating key pathways in stress tolerance or biosynthesis of the metabolites of interest were limited and proportional to the economic weight of each species. Finally, by aligning 53 plastid genomes from as many species as possible, we demonstrated the precision offered by the super barcoding approach to reconstruct the phylogenetic relationships of Apiaceae species. Overall, despite the impressive size of this family, we documented an evident lack of molecular data, especially because genomic and transcriptomic resources are circumscribed to a small number of species. We believe that our contribution can help future studies aimed at developing molecular tools for boosting breeding programs in crop plants of the Apiaceae family.
Liquid Biopsy in Melanoma: Significance in Diagnostics, Prediction and Treatment Monitoring
Authors: Kamińska Paula , Buszka Karolina , Zabel Maciej , Nowicki Michał , Alix-Panabières Catherine , Budna-Tukan Joanna
Published: September 2021

Liquid biopsy is a common term referring to circulating tumor cells and other biomarkers, such as circulating tumor DNA (ctDNA) or extracellular vesicles. Liquid biopsy presents a range of clinical advantages, such as the low invasiveness of the blood sample collection and continuous control of the tumor progression. In addition, this approach enables the mechanisms of drug resistance to be determined in various methods of cancer treatment, including immunotherapy. However, in the case of melanoma, the application of liquid biopsy in patient stratification and therapy needs further investigation. This review attempts to collect all of the relevant and recent information about circulating melanoma cells (CMCs) related to the context of malignant melanoma and immunotherapy. Furthermore, the biology of liquid biopsy analytes, including CMCs, ctDNA, mRNA and exosomes, as well as techniques for their detection and isolation, are also described. The available data support the notion that thoughtful selection of biomarkers and technologies for their detection can contribute to the development of precision medicine by increasing the efficacy of cancer diagnostics and treatment.
Early Embryo Exposure to Assisted Reproductive Manipulation Induced Subtle Changes in Liver Epigenetics with No Apparent Negative Health Consequences in Rabbit
Authors: García-Domínguez Ximo , Diretto Gianfranco , Peñaranda David S. , Frusciante Sarah , García-Carpintero Victor , Cañizares Joaquín , Vicente José S. , Marco-Jiménez Francisco
Published: September 2021

Embryo manipulation is a requisite step in assisted reproductive technology (ART). Therefore, it is of great necessity to appraise the safety of ART and investigate the long-term effect, including lipid metabolism, on ART-conceived offspring. Augmenting our ART rabbit model to investigate lipid metabolic outcomes in offspring longitudinally, we detected variations in hepatic DNA methylation ART offspring in the F3 generation for embryonic exposure (multiple ovulation, vitrification and embryo transfer). Through adult liver metabolomics and proteomics, we identified changes mainly related to lipid metabolism (e.g., polyunsaturated fatty acids, steroids, steroid hormone). We also found that DNA methylation analysis was linked to changes in lipid metabolism and apoptosis genes. Nevertheless, these differences did not apparently alter the general health status. Thus, our findings suggest that ART is likely to be a player in embryo epigenetic events related to hepatic homeostasis alteration in adulthood.
Small Molecules Targeting Programmed Cell Death in Breast Cancer Cells
Authors: Maniam Subashani , Maniam Sandra
Published: September 2021

Targeted chemotherapy has become the forefront for cancer treatment in recent years. The selective and specific features allow more effective treatment with reduced side effects. Most targeted therapies, which include small molecules, act on specific molecular targets that are altered in tumour cells, mainly in cancers such as breast, lung, colorectal, lymphoma and leukaemia. With the recent exponential progress in drug development, programmed cell death, which includes apoptosis and autophagy, has become a promising therapeutic target. The research in identifying effective small molecules that target compensatory mechanisms in tumour cells alleviates the emergence of drug resistance. Due to the heterogenous nature of breast cancer, various attempts were made to overcome chemoresistance. Amongst breast cancers, triple negative breast cancer (TNBC) is of particular interest due to its heterogeneous nature in response to chemotherapy. TNBC represents approximately 15% of all breast tumours, however, and still has a poor prognosis. Unlike other breast tumours, signature targets lack for TNBCs, causing high morbidity and mortality. This review highlights several small molecules with promising preclinical data that target autophagy and apoptosis to induce cell death in TNBC cells.
Sarcopenia: Etiology, Nutritional Approaches, and miRNAs
Authors: Cannataro Roberto , Carbone Leandro , Petro Jorge L. , Cione Erika , Vargas Salvador , Angulo Heidy , Forero Diego A. , Odriozola-Martínez Adrián , Kreider Richard B. , Bonilla Diego A.
Published: September 2021

Sarcopenia, an age-related decline in skeletal muscle mass and function, dramatically affects the quality of life. Although there is a consensus that sarcopenia is a multifactorial syndrome, the etiology and underlying mechanisms are not yet delineated. Moreover, research about nutritional interventions to prevent the development of sarcopenia is mainly focused on the amount and quality of protein intake. The impact of several nutrition strategies that consider timing of food intake, anti-inflammatory nutrients, metabolic control, and the role of mitochondrial function on the progression of sarcopenia is not fully understood. This narrative review summarizes the metabolic background of this phenomenon and proposes an integral nutritional approach (including dietary supplements such as creatine monohydrate) to target potential molecular pathways that may affect reduce or ameliorate the adverse effects of sarcopenia. Lastly, miRNAs, in particular those produced by skeletal muscle (MyomiR), might represent a valid tool to evaluate sarcopenia progression as a potential rapid and early biomarker for diagnosis and characterization.
In Vitro Model to Investigate Communication between Dorsal Root Ganglion and Spinal Cord Glia
Authors: Ma Junxuan , Patil Vaibhav , Pandit Abhay , Quinlan Leo R. , Finn David P. , Grad Sibylle , Alini Mauro , Peroglio Marianna
Published: September 2021

Chronic discogenic back pain is associated with increased inflammatory cytokine levels that can influence the proximal peripheral nervous system, namely the dorsal root ganglion (DRG). However, transition to chronic pain is widely thought to involve glial activation in the spinal cord. In this study, an in vitro model was used to evaluate the communication between DRG and spinal cord glia. Primary neonatal rat DRG cells were treated with/without inflammatory cytokines (TNF-α, IL-1β, and IL-6). The conditioned media were collected at two time points (12 and 24 h) and applied to spinal cord mixed glial culture (MGC) for 24 h. Adult bovine DRG and spinal cord cell cultures were also tested, as an alternative large animal model, and results were compared with the neonatal rat findings. Compared with untreated DRG-conditioned medium, the second cytokine-treated DRG-conditioned medium (following medium change, thus containing solely DRG-derived molecules) elevated CD11b expression and calcium signal in neonatal rat microglia and enhanced Iba1 expression in adult bovine microglia. Cytokine treatment induced a DRG-mediated microgliosis. The described in vitro model allows the use of cells from large species and may represent an alternative to animal pain models (3R principles).
The Role of Melanoma Cell-Derived Exosomes (MTEX) and Photodynamic Therapy (PDT) within a Tumor Microenvironment
Authors: Mkhobongo Bridgette , Chandran Rahul , Abrahamse Heidi
Published: September 2021

Photodynamic Therapy (PDT), an unconventional cancer therapy with optimistic desirable effects, utilizes the delivery of a photosensitizer (PS) that is activated by light at a particular wavelength and inducing oxidative cytotoxic damage of a tumor and its surrounding vasculature. Deeper seated tumors such as internally metastasized melanomas are more difficult to treat with PDT as the penetration of laser light to those sites is less. Limitations in targeting melanomas can also be attributed to melanin pigments that hinder laser light from reaching targeted sites. Exosomes serve as naturally occurring nanoparticles that can be re-assembled with PSs, improving targeted cellular absorption of photosensitizing agents during PDT. Additionally, studies indicate that exosomes released from PDT-treated tumor cells play a critical role in mediating anti-tumor immune responses. This review collates the role of Melanoma Cell-Derived Exosomes (MTEX) in immune response mediation and metastasis. Tumor Cell-Derived Exosomes (TEX) post PDT treatment are also reviewed, as well as the effects of exosomes as carriers of photosensitizers and delivery systems for PDT. The understanding and research on the role of melanoma exosomes induced by Photodynamic Therapy and their tumor microenvironment will assist in future research in treatment prospects and implications.
Targeted Delivery of Zinc Pyrithione to Skin Epithelia
Authors: Mangion Sean E. , Holmes Amy M. , Roberts Michael S.
Published: September 2021

Zinc pyrithione (ZnPT) is an anti-fungal drug delivered as a microparticle to skin epithelia. It is one of the most widely used ingredients worldwide in medicated shampoo for treating dandruff and seborrheic dermatitis (SD), a disorder with symptoms that include skin flaking, erythema and pruritus. SD is a multi-factorial disease driven by microbiol dysbiosis, primarily involving Malassezia yeast. Anti-fungal activity of ZnPT depends on the cutaneous availability of bioactive monomeric molecular species, occurring upon particle dissolution. The success of ZnPT as a topical therapeutic is underscored by the way it balances treatment efficacy with formulation safety. This review demonstrates how ZnPT achieves this balance, by integrating the current understanding of SD pathogenesis with an up-to-date analysis of ZnPT pharmacology, therapeutics and toxicology. ZnPT has anti-fungal activity with an average in vitro minimum inhibitory concentration of 10–15 ppm against the most abundant scalp skin Malassezia species (Malassezia globosa and Malassezia restrica). Efficacy is dependent on the targeted delivery of ZnPT to the skin sites where these yeasts reside, including the scalp surface and hair follicle infundibulum. Imaging and quantitative analysis tools have been fundamental for critically evaluating the therapeutic performance and safety of topical ZnPT formulations. Toxicologic investigations have focused on understanding the risk of local and systemic adverse effects following exposure from percutaneous penetration. Future research is expected to yield further advances in ZnPT formulations for SD and also include re-purposing towards a range of other dermatologic applications, which is likely to have significant clinical impact.
Plant Viral Nanoparticle Conjugated with Anti-PD-1 Peptide for Ovarian Cancer Immunotherapy
Authors: Gautam Aayushma , Beiss Veronique , Wang Chao , Wang Lu , Steinmetz Nicole F.
Published: September 2021

Immunotherapy holds tremendous potential in cancer therapy, in particular, when treatment regimens are combined to achieve synergy between pathways along the cancer immunity cycle. In previous works, we demonstrated that in situ vaccination with the plant virus cowpea mosaic virus (CPMV) activates and recruits innate immune cells, therefore reprogramming the immunosuppressive tumor microenvironment toward an immune-activated state, leading to potent anti-tumor immunity in tumor mouse models and canine patients. CPMV therapy also increases the expression of checkpoint regulators on effector T cells in the tumor microenvironment, such as PD-1/PD-L1, and we demonstrated that combination with immune checkpoint therapy improves therapeutic outcomes further. In the present work, we tested the hypothesis that CPMV could be combined with anti-PD-1 peptides to replace expensive antibody therapies. Specifically, we set out to test whether a multivalent display of anti-PD-1 peptides (SNTSESF) would enhance efficacy over a combination of CPMV and soluble peptide. Efficacy of the approaches were tested using a syngeneic mouse model of intraperitoneal ovarian cancer. CPMV combination with anti-PD-1 peptides (SNTSESF) resulted in increased efficacy; however, increased potency against metastatic ovarian cancer was only observed when SNTSESF was conjugated to CPMV, and not added as a free peptide. This can be explained by the differences in the in vivo fates of the nanoparticle formulation vs. the free peptide; the larger nanoparticles are expected to exhibit prolonged tumor residence and favorable intratumoral distribution. Our study provides new design principles for plant virus-based in situ vaccination strategies.
Climate Change and Major Pests of Mediterranean Olive Orchards: Are We Ready to Face the Global Heating?
Authors: Caselli Alice , Petacchi Ruggero
Published: September 2021

Evidence of the impact of climate change on natural and agroecosystems is nowadays established worldwide, especially in the Mediterranean Basin, an area known to be very susceptible to heatwaves and drought. Olea europaea is one of the main income sources for the Mediterranean agroeconomy, and it is considered a sensitive indicator of the climate change degree because of the tight relationship between its biology and temperature trend. Despite the economic importance of the olive, few studies are nowadays available concerning the consequences that global heating may have on its major pests. Among the climatic parameters, temperature is the key one influencing the relation between the olive tree and its most threatening parasites, including Bactrocera oleae and Prays oleae. Therefore, several prediction models are based on this climatic parameter (e.g., cumulative degree day models). Even if the use of models could be a promising tool to improve pest control strategies and to safeguard the Mediterranean olive patrimony, they are not currently available for most O. europaea pests, and they have to be used considering their limits. This work stresses the lack of knowledge about the biology and the ethology of olive pests under a climate change scenario, inviting the scientific community to focus on the topic.
Long-Term Population Dynamics of Namib Desert Tenebrionid Beetles Reveal Complex Relationships to Pulse-Reserve Conditions
Authors: Henschel Joh R.
Published: September 2021

Noy-Meir’s paradigm concerning desert populations being predictably tied to unpredictable productivity pulses was tested by examining abundance trends of 26 species of flightless detritivorous tenebrionid beetles (Coleoptera, Tenebrionidae) in the hyper-arid Namib Desert (MAP = 25 mm). Over 45 years, tenebrionids were continuously pitfall trapped on a gravel plain. Species were categorised according to how their populations increased after 22 effective rainfall events (>11 mm in a week), and declined with decreasing detritus reserves (97.7–0.2 g m−2), while sustained by nonrainfall moisture. Six patterns of population variation were recognised: (a) increases triggered by effective summer rainfalls, tracking detritus over time (five species, 41% abundance); (b) irrupting upon summer rainfalls, crashing a year later (three, 18%); (c) increasing gradually after series of heavy (>40 mm) rainfall years, declining over the next decade (eight, 15%); (d) triggered by winter rainfall, population fluctuating moderately (two, 20%); (e) increasing during dry years, declining during wet (one, 0.4%); (f) erratic range expansions following heavy rain (seven, 5%). All species experienced population bottlenecks during a decade of scant reserves, followed by the community cycling back to its earlier composition after 30 years. By responding selectively to alternative configurations of resources, Namib tenebrionids showed temporal patterns and magnitudes of population fluctuation more diverse than predicted by Noy-Meir’s original model, underpinning high species diversity.
Clinical and Cytokine Profile in Patients with Early and Late Onset Meniere Disease
Authors: Moleon Maria-Del-Carmen , Martinez-Gomez Estrella , Flook Marisa , Peralta-Leal Andreina , Gallego Juan Antonio , Sanchez-Gomez Hortensia , Montilla-Ibañez Maria Alharilla , Dominguez-Durán Emilio , Soto-Varela Andres , Aran Ismael , Frejo Lidia , Lopez-Escamez Jose A.
Published: September 2021

Background: Meniere disease (MD) is an inner ear disorder associated with comorbidities such as autoimmune diseases or migraine. This study describes clinical and cytokine profiles in MD according to the age of onset of the condition. Methods: A cross-sectional study including 83 MD patients: 44 with early-onset MD (EOMD, <35 years old), and 39 with late-onset MD (LOMD, >50 years old), 64 patients with migraine and 55 controls was carried out. Clinical variables and cytokines levels of CCL3, CCL4, CCL18, CCL22, CXCL,1 and IL-1β were compared among the different groups. Results: CCL18 levels were higher in patients with migraine or MD than in controls. Elevated levels of IL-1β were observed in 11.4% EOMD and in 10.3% LOMD patients and these levels were not dependent on the age of individuals. EOMD had a longer duration of the disease (p = 0.004) and a higher prevalence of migraine than LOMD (p = 0.045). Conclusions: Patients with EOMD have a higher prevalence of migraine than LOMD, but migraine is not associated with any cytokine profile in patients with MD. The levels of CCL18, CCL3, and CXCL4 were different between patients with MD or migraine and controls.
HBV and HDV: New Treatments on the Horizon
Authors: Zuccaro Valentina , Asperges Erika , Colaneri Marta , Marvulli Lea Nadia , Bruno Raffaele
Published: September 2021

Despite the accumulating knowledge, chronic hepatitis B (CHB) and HDV infection represent a global health problem, and there are still several critical issues, which frequently remain uncovered. In this paper, we provided an overview of the current therapeutic options and summarized the investigational therapies in the pipeline. Furthermore, we discussed some critical issues such as a “functional cure” approach, the futility of long-term NA therapy and the relevance of understanding drug actions and safety of antivirals, especially in special populations.
Chronic Myeloid Leukemia in Children: Immune Function and Vaccinations
Authors: Suttorp Meinolf , Webster Carrion Andrea , Hijiya Nobuko
Published: September 2021

Children with CML need TKI treatment for many years, and the lack of knowledge about immune dysfunction with TKI has hindered routine immunizations. This review attempts to provide an overview of the effects of TKIs licensed for children (e.g., imatinib, dasatinib, and nilotinib) on immune function, as well as its implications on immunizations. We discuss surveillance strategies (e.g., immunoglobulin blood serum levels and hepatitis B reactivation) and immunizations. All inactivated vaccines (e.g., influenza, pneumococcal, and streptococcal) can be given during the treatment of CML in the chronic phase, although their efficacy may be lower. As shown in single cases of children and adults with CML, live vaccines (e.g., varicella, measles, mumps, rubella, and yellow fever) may be administered under defined circumstances with great precautions. We also highlight important aspects of COVID-19 in this patient population (e.g., the outcome of COVID-19 infection in adults with CML and in children with varying hemato-oncological diseases) and discuss the highly dynamic field of presently available different vaccination options. In conclusion, TKI treatment for CML causes humoral and cellular immune dysfunction, which is mild in most patients, and thus infectious complications are rare. Routine immunizations are important for health maintenance of children, but vaccinations for children with CML on TKI therapy should be carefully considered.
Does Mandibular Gonial Angle Influence the Eruption Pattern of the Lower Third Molar? A Three-Dimensional Study
Authors: Barone Selene , Antonelli Alessandro , Averta Fiorella , Diodati Federica , Muraca Danila , Bennardo Francesco , Giudice Amerigo
Published: September 2021

Lower third molars (M3M) are the most frequent impacted teeth. The aim of this study was to evaluate the correlation between M3M position and gonial angle. A retrospective cross-sectional study was conducted. The study population included patients with unilateral or bilateral M3M and underwent Cone Beam Computed Tomography. A morphometric analysis of the mandible was performed after three-dimensional reconstruction, recording gonial angle (GA), ramus high, ramus width, ramus divergency, and retromolar space. GA was the primary predictor variable. The primary outcome variable was the position of M3M analyzed in sagittal, axial, and coronal planes. Descriptive, bivariate, and multiple regression statistics were performed (p < 0.05). Study sample included 172 patients (mean age: 26.3 ± 4.6 years); 266 M3Ms were analyzed. The average GA was 122.6° ± 4.8°. A reduced GA value was significantly associated with a deeply impacted M3M in the ramus. With a progressive decrease of GA, M3M assumed a more horizontal position closer to the mandibular canal (p < 0.05). A lower GA showed a reduced retromolar space with more complex impacted M3M (p < 0.05). The results confirm a statistically significant correlation between GA and the position of M3M. Higher incidence of impacted M3M was related to a reduction of the GA value.
COVID-19-Induced Reduction in Primary Melanoma Diagnoses: Experience from a Dermatopathology Referral Center
Authors: Hoellwerth Magdalena , Kaiser Andreas , Emberger Michael , Brandlmaier Matthias , Laimer Martin , Egger Alexander , Bauer Johann W. , Koelblinger Peter
Published: September 2021

The collateral damage caused by COVID-19 pandemic-associated public health and governmental measures on patient care has been increasingly assessed in various oncological and non-oncological clinical settings. In order to investigate potential adverse effects in the field of melanoma the present study analyzed the characteristics of primary melanoma diagnoses at an Austrian dermato-pathological referral center before, during, and after the first coronavirus-related lockdown in March 2020. As suspected, we found significant temporary reductions in the number of newly diagnosed melanomas in 2020 compared to previous years, in particular, during the first lockdown period.
Selective Arterial Embolization of Renal Angiomyolipomas with a N-Butyl Cyanoacrylate-Lipiodol Mixture: Efficacy, Safety, Short- and Mid-Term Outcomes
Authors: Prigent François-Victor , Guillen Kévin , Comby Pierre-Olivier , Pellegrinelli Julie , Falvo Nicolas , Midulla Marco , Majbri Nabil , Chevallier Olivier , Loffroy Romaric
Published: September 2021

Selective arterial embolization (SAE) for renal angiomyolipoma (rAML) is effective to treat or prevent bleeding. We report our experience using a cyanoacrylate–Lipiodol mixture. We performed a single-center retrospective review of all rAMLs embolized with cyanoacrylate glue between July 2014 and June 2020. Demographics, tuberous sclerosis complex (TSC) status, clinical presentation, angiography features, and follow-up data were recorded. Pre- and post-procedure rAML sizes and volumes were estimated from computed tomography (CT) or magnetic resonance imaging (MRI) studies. Kidney function was assessed before and after the procedure. We identified 24 patients (22 females and 2 males, mean age 51 years) treated for 27 AMLs, either prophylactically (n = 20) or as an emergency (n = 4). Technical success was achieved for 25/27 AMLs; two patients, each with a single AML, required nephrectomy and repeated embolization, respectively. Major complications occurred in three patients and minor complications such as postembolization syndrome in 15 patients. AML volume reduction after embolization was 55.1% after a mean follow-up of 15 months (range, 1–72 months). Factors associated with greater volume reduction were a smaller percentage of fat (p = 0.001), larger initial rAML volume (p = 0.014), and longer follow-up (p = 0.0001). The mean creatinine level did not change after SAE. Embolization of rAMLs with a mixture of cyanoacrylate and Lipiodol is feasible, safe, and effective in significantly decreasing tumor volume.
Impact of the Vaginal and Endometrial Microbiome Pattern on Assisted Reproduction Outcomes
Authors: Diaz-Martínez María del Carmen , Bernabeu Andrea , Lledó Belén , Carratalá-Munuera Concepción , Quesada Jose A. , Lozano Francisca M. , Ruiz Vicente , Morales Ruth , Llácer Joaquín , Ten Jorge , Castillo Juan Carlos , Rodríguez Adoración , Nouni-García Rauf , López-Pineda Adriana , Moliner Belén , Bernabeu Rafael
Published: September 2021

Uterine microbiota may be involved in reproductive health and disease. This study aims to describe and compare the vaginal and endometrial microbiome patterns between women who became pregnant and women who did not after in vitro fertilization. We also compared the vaginal and endometrial microbiome patterns between women with and without a history of repeated implantation failures (RIF). This pilot prospective cohort study included 48 women presenting to the fertility clinic for IVF from May 2017 to May 2019. Women who achieved clinical pregnancy presented a greater relative abundance of Lactobacillus spp. in their vaginal samples than those who did not (97.69% versus 94.63%; p = 0.027. The alpha and beta diversity of vaginal and endometrial samples were not statistically different between pregnant and non-pregnant women. The Faith alpha diversity index in vaginal samples was lower in women with RIF than those without RIF (p = 0.027). The alpha diversity of the endometrial microbiome was significantly higher in women without RIF (p = 0.021). There were no significant differences in the vaginal and endometrial microbiomes between pregnant and non-pregnant women. The relative abundance of the genera in women with RIF was different from those without RIF. Statistically significant differences in the endometrial microbiome were found between women with and without RIF.
Tor Hidden Services: A Systematic Literature Review
Authors: Huete Trujillo Diana L. , Ruiz-Martínez Antonio
Published: September 2021

Anonymous communications networks were created to protect the privacy of communications, preventing censorship and traffic analysis. The most famous anonymous communication network is Tor. This anonymous communication network provides some interesting features. Among them, we can mention that Tor can hide a user’s IP address when accessing to a service such as the Web, and it also supports Tor hidden services (THS) (now named onion services) as a mechanism to conceal the server’s IP address, used mainly to provide anonymity to websites. THS is an important research field in Tor. However, there is a lack of reviews that sum up the main findings and research challenges. In this article, we present a systematic literature review that aims to offer a comprehensive overview of the research made on THS by presenting the state-of-the-art and the different research challenges to be addressed. This review has been developed from a selection of 57 articles and presents main findings and advances regarding Tor hidden services, limitations found, and future issues to be investigated.
Hydrogen Bonds in Blends of Poly(N-isopropylacrylamide), Poly(N-ethylacrylamide) Homopolymers, and Carboxymethyl Cellulose
Authors: García-Peñas Alberto , Liang Weijun , Hashmi Saud , Sharma Gaurav , Saeb Mohammad Reza , Stadler Florian J.
Published: September 2021

Recently, it was reported that the physical crosslinking exhibited by some biopolymers could provide multiple benefits to biomedical applications. In particular, grafting thermoresponsive polymers onto biopolymers may enhance the degradability or offer other features, as thermothickening behavior. Thus, different interactions will affect the different hydrogen bonds and interactions from the physical crosslinking of carboxymethyl cellulose, the lower critical solution temperatures (LCSTs), and the presence of the ions. This work focuses on the study of blends composed of poly(N-isopropylacrylamide), poly(N-ethylacrylamide), and carboxymethyl cellulose in water and water/methanol. The molecular features, thermoresponsive behavior, and gelation phenomena are deeply studied. The ratio defined by both homopolymers will alter the final properties and the gelation of the final structures, showing that the presence of the hydrophilic groups modifies the number and contributions of the diverse hydrogen bonds.
Pulsed Mechanical Surface Treatment—An Approach to Combine the Advantages of Shot Peening, Deep Rolling, and Machine Hammer Peening
Authors: Meyer Daniel , Hettig Matthias , Mensching Nicole
Published: September 2021

Several manufacturing processes are used to beneficially influence the surface and subsurface properties of manufactured parts. Different aspects such as the surface topography or resulting residual stresses are addressed using different manufacturing processes. This paper presents the first approach for pulsed mechanical surface treatment (PMST), a new manufacturing process aiming to combine the mechanics used in deep rolling and shot or hammer peening. The process can generate a defined surface topography while constantly impinging a mechanical impact on the workpiece. Two different tools (type 1 and type 2) have been designed to approach this new concept. Hardened carbide pins are used for type 1 to prove the concept using a simpler kinematic and resulting in a burnishing-like process. For type 2, hardened roller is used and results in an actual rolling process. Specimens made of S235 are processed in experiments with tool type 1 with varying pulse frequency and feeds. The resulting surface topography is described using optical measurement systems while micro-hardness measurements are used to describe the subsurface properties. The results in general show an increase of hardness in the surface and subsurface layer while the resulting surface topography can be directly controlled by the process parameters and therefore be designed for specific functional properties.
Occurrence of Microplastics in the Gastrointestinal Tract and Gills of Fish from Guangdong, South China
Authors: Pan Zhengkun , Zhang Chaonan , Wang Shaodan , Sun Di , Zhou Aiguo , Xie Shaolin , Xu Guohuan , Zou Jixing
Published: September 2021

Microplastic pollution has become a major global concern. Coastal areas are densely populated with human activity, commercial enterprises, and fishing, resulting in high incidences of fish microplastic pollution. It has been shown that microplastics exist in commercial fish in coastal areas of Guangdong, China. Most of the microplastics were less than 1 mm, white and blue fibers. The abundance, type, shape and color of microplastics in gills and in the gastrointestinal tract (GIT) of eight species of commercial fish in the coastal waters of central and western Guangdong Province were analyzed. The overall abundance of microplastic particles in fish was 6.6 items/individual, with an average of 2.2 particles in gills and 4.4 in the GIT. The GIT of carnivorous fish was less likely to ingest microplastics than that of herbivores or omnivores. Middle-water fish, on the other hand, are less likely to have their gills contaminated with microplastics. These results revealed the degree of microplastic pollution in fish tissues from the central and western coastal areas of Guangdong province. The quantity of microplastics in the GIT of fish in the Pearl River Delta was the highest compared with the surrounding areas.
Numerical Simulation of the Motion of a Large Scale Unmanned Surface Vessel in High Sea State Waves
Authors: Huang Shuo , Liu Weiqi , Luo Wanzhen , Wang Kai
Published: September 2021

The motion stability of the Unmanned Surface Vessel (USV) is threatened by the action of waves under a rough sea state. In the present paper, the motion of a large-scale USV is numerically simulated under high sea state of level 5 and 7. The overset grid method and Reynolds Averaged Navier–Stokes (RANS) approach are employed to solve Navier–Stokes (N-S) equations. For the case of wave incident angle 0° and 30°, the heave, pitch and roll motion response of a large scale USV are investigated by using the six Degrees of Freedom (6-DOF) numerical model. The effects of different sea states, as well as different wave directions, on the motion of USV are compared. The comparative results indicate that the response of this USV in waves is the periodic free-motion according to the corresponding amplitude, which does not exceed the stable range, and there are no overturning and other situations that may affect the safety, in the case of level 5 and 7 sea states. The corresponding pressure at the bottom of this USV meets the range of material strength, and no structural damage or injury to the hull occurs, although the pressure varies at different wave periods. For the case of different wave directions, the analysis of the boundary layer thickness shows that the wave direction is of great importance to the boundary layer thickness distribution, both in the level 5 and level 7 sea states.
Beachrock as a Paleoshoreline Indicator: Example from Wadi Al-Hamd, South Al-Wajh, Saudi Arabia
Authors: Mannaa Ammar A. , Haredy Rabea A. , Ghandour Ibrahim M.
Published: September 2021

The present study concerns the Holocene inland beachrocks that are exposed in the Red Sea coastal plain at the mouth of Wadi Al-Hamd, South Al-Wajh City, Saudi Arabia, and their utility as an indicator for Holocene climate and sea level changes. In addition, the framework composition, and carbon and oxygen isotopic data, are employed to interpret the origin of their cement. The beachrock consists mainly of gravel and coarse-grained terrigenous sediments dominated by lithic fragments of volcanic rocks, cherts and rare limestones along with quartz, feldspars and traces of amphiboles and heavy minerals. In addition, rare skeletal remains dominated by coralline algae, benthic foraminifera and mollusca remains are recognized. The allochems are cemented by high Mg-calcite (HMC) formed mainly in the intertidal zone under active marine phreatic conditions. The cement takes the form of isopachous to anisopachous rinds of bladed crystals, micritic rim non-selectively surrounding siliciclastic and skeletal remains, and pore-filling micrite. Pore-filling micrite cement occasionally displays a meniscus fabric, suggesting a vadose environment. The δ18O and δ13C values of carbonate cement range from −0.35‰ to 1‰ (mean 0.25‰) and −0.09‰ to 3.03‰ (mean 1.85‰), respectively, which are compatible with precipitation from marine waters. The slight depletion in δ18O and δ13C values in the proximal sample may suggest a slight meteoric contribution.