bluuebunny/crossref_metadata_2025 huggingface dataset:
Sub-bandgap near-infrared photovoltaic response in Au/Al2O3/n-Si metal–insulator–semiconductor structure by plasmon-enhanced internal photoemission
Authors: Dai Xiyuan , Wu Li , Yu Liang , Yu Zhiyuan , Ma Fengyang , Zhang Yuchen , Yang Yanru , Sun Jian , Lu Ming
Published: March 2023

Silicon sub-bandgap near-infrared (NIR) (λ > 1100 nm) photovoltaic (PV) response by plasmon-enhanced internal photoemission was investigated. The Si sub-bandgap NIR PV response, which remains unexploited in Schottky junction-like solar cell device, was examined using nanometer sized Au/Al2O3/n-Si junction arrays. This kind of metal–insulator–semiconductor structure was similar in functionality to Schottky junction in NIR absorption, photo-induced charge separation and collection. It showed that NIR absorption increased steadily with increasing volume of Au nanoparticles (NPs) till a saturation was reached. Simulation results indicated the formation of localized surface plasmon on the surfaces of Au NPs, which was correlated well with the observed NIR absorption. On the other hand, the NIR PV response was found sensitive to the amount and size of Au NPs and thickness of Al2O3. Chemical and field-effect passivation of n-Si by using Al2O3and SiO2were used to optimize the NIR PV response. In the current configuration, the best PV conversion efficiency was 0.034% atλ = 1319 nm under illumination power of 0.1 W/cm2.
Comparison of the Illumina NextSeq 2000 and GeneMind Genolab M sequencing platforms for spatial transcriptomics
Authors: Pavel Iamshchikov , Irina Larionova , Tatiana Gerashchenko , Denis Piankov , Philipp Koshkin , Sergei Korostelev , Evgeny Denisov
Published: March 2023

Background

The Illumina sequencing systems demonstrate high efficiency and power and remain the most popular platforms. Platforms with similar throughput and quality profiles but lower costs are under intensive development. In this study, we compared two platforms Illumina NextSeq 2000 and GeneMind Genolab M for 10x Genomics Visium spatial transcriptomics.

Results

The performed comparison demonstrates that GeneMind Genolab M sequencing platform produces highly consistent with Illumina NextSeq 2000 sequencing results. Both platforms have similar performance in terms of sequencing quality and detection of UMI, spatial barcode, and probe sequence. Raw read mapping and following read counting produced highly comparable results that is confirmed by quality control metrics and strong correlation between expression profiles in the same tissue spots. Downstream analysis including dimension reduction and clustering demonstrated similar results, and differential gene expression analysis predominantly detected the same genes for both platforms.

Conclusions

GeneMind Genolab M instrument is similar to Illumina sequencing efficacy and is suitable for 10x Genomics Visium spatial transcriptomics.
Low tidal volume ventilation for patients undergoing laparoscopic surgery: a secondary analysis of a randomised clinical trial
Authors: Karalapillai Dharshi , Weinberg Laurence , Neto Ary Serpa , Peyton Philip J. , Ellard Louise , Hu Raymond , Pearce Brett , Tan Chong O. , Story David , O’Donnell Mark , Hamilton Patrick , Oughton Chad , Galtieri Jonathan , Wilson Anthony , Liskaser Grace , Balasubramaniam Ajay , Eastwood Glenn , Bellomo Rinaldo , Jones Daryl A.
Published: March 2023

Background

We recently reported the results for a large randomized controlled trial of low tidal volume ventilation (LTVV) versus conventional tidal volume (CTVV) during major surgery when positive end expiratory pressure (PEEP) was equal between groups. We found no difference in postoperative pulmonary complications (PPCs) in patients who received LTVV. However, in the subgroup of patients undergoing laparoscopic surgery, LTVV was associated with a numerically lower rate of PPCs after surgery. We aimed to further assess the relationship between LTVV versus CTVV during laparoscopic surgery.

Methods

We conducted a post-hoc analysis of this pre-specified subgroup. All patients received volume-controlled ventilation with an applied PEEP of 5 cmH2O and either LTVV (6 mL/kg predicted body weight [PBW]) or CTVV (10 mL/kg PBW). The primary outcome was the incidence of a composite of PPCs within seven days.

Results

Three hundred twenty-eight patients (27.2%) underwent laparoscopic surgeries, with 158 (48.2%) randomised to LTVV. Fifty two of 157 patients (33.1%) assigned to LTVV and 72 of 169 (42.6%) assigned to conventional tidal volume developed PPCs within 7 days (unadjusted absolute difference, − 9.48 [95% CI, − 19.86 to 1.05]; p = 0.076). After adjusting for pre-specified confounders, the LTVV group had a lower incidence of the primary outcome than patients receiving CTVV (adjusted absolute difference, − 10.36 [95% CI, − 20.52 to − 0.20]; p = 0.046).

Conclusion

In this post-hoc analysis of a large, randomised trial of LTVV we found that during laparoscopic surgeries, LTVV was associated with a significantly reduced PPCs compared to CTVV when PEEP was applied equally between both groups.

Trial registration

Australian and New Zealand Clinical Trials Registry no: 12614000790640.
Physical activity levels in cognitively normal and cognitively impaired oldest-old and the association with dementia risk factors: a pilot study
Authors: Muurling Marijn , Badissi Maryam , de Boer Casper , Legdeur Nienke , Barkhof Frederik , van Berckel Bart N.M. , Maier Andrea B. , Pijnappels Mirjam , Visser Pieter Jelle
Published: March 2023

Background

Research assessing the relationship of physical activity and dementia is usually based on studies with individuals younger than 90 years of age. The primary aim of this study was to determine physical activity levels of cognitively normal and cognitively impaired adults older than 90 years of age (oldest-old). Our secondary aim was to assess if physical activity is associated with risk factors for dementia and brain pathology biomarkers.

Methods

Physical activity was assessed in cognitively normal (N = 49) and cognitively impaired (N = 12) oldest-old by trunk accelerometry for a 7-day period. We tested physical performance parameters and nutritional status as dementia risk factors, and brain pathology biomarkers. Linear regression models were used to examine the associations, correcting for age, sex and years of education.

Results

Cognitively normal oldest-old were on average active for a total duration of 45 (SD 27) minutes per day, while cognitively impaired oldest-old seemed less physically active with 33 (SD 21) minutes per day with a lower movement intensity. Higher active duration and lower sedentary duration were related to better nutritional status and better physical performance. Higher movement intensities were related to better nutritional status, better physical performance and less white matter hyperintensities. Longer maximum walking bout duration associated with more amyloid binding.

Conclusion

We found that cognitively impaired oldest-old are active at a lower movement intensity than cognitively normal oldest-old individuals. In the oldest-old, physical activity is related to physical parameters, nutritional status, and moderately to brain pathology biomarkers.
Somatic mutation landscape in a cohort of meningiomas that have undergone grade progression
Authors: Cain Sarah A , Pope Bernard , Mangiola Stefano , Mantamadiotis Theo , Drummond Katharine J
Published: March 2023

Background

A subset of meningiomas progress in histopathological grade but drivers of progression are poorly understood. We aimed to identify somatic mutations and copy number alterations (CNAs) associated with grade progression in a unique matched tumour dataset.

Methods

Utilising a prospective database, we identified 10 patients with meningiomas that had undergone grade progression and for whom matched pre- and post-progression tissue (n = 50 samples) was available for targeted next-generation sequencing.

Results

Mutations inNF2were identified in 4/10 patients, of these 94% were non-skull base tumours. In one patient, three differentNF2mutations were identified in four tumours.NF2mutated tumours showed large-scale CNAs, with highly recurrent losses in 1p, 10, 22q, and frequent CNAs on chromosomes 2, 3 and 4. There was a correlation between grade and CNAs in two patients. Two patients with tumours without detectedNF2mutations showed a combination of loss and high gain on chromosome 17q. Mutations inSETD2,TP53,TERTpromoter andNF2were not uniform across recurrent tumours, however did not correspond with the onset of grade progression.

Conclusion

Meningiomas that progress in grade generally have a mutational profile already detectable in the pre-progressed tumour, suggesting an aggressive phenotype. CNA profiling shows frequent alterations inNF2mutated tumours compared to nonNF2mutated tumours. The pattern of CNAs may be associated with grade progression in a subset of cases.
Genotype-phenotype correlations of STXBP1 pathogenic variants and the treatment choices for STXBP1-related disorders in China
Authors: Kessi Miriam , Chen Baiyu , Shan Li-Dan , Wang Ying , Yang Lifen , Yin Fei , He Fang , Peng Jing , Wang Guoli
Published: March 2023

Background

We aimed to analyze the genotype-phenotype correlations of STXBP1 pathogenic variants, prognostic factors and the treatment choices in a case-series of STXBP1-related disorders from China.

Methods

The clinical data and genetic results of the children diagnosed with STXBP1-related disorders at Xiangya hospital from 2011 to 2019 were collected retrospectively, and analyzed. We divided our patients into groups for comparison purposes: patients with missense variants and nonsense variants, patients who are seizure-free and not seizure-free, patients with mild to moderate intellectual disability (ID) and severe to profound global developmental delay (GDD).

Results

Nineteen patients were enrolled: 17 (89.5%) unrelated and 2 (10.5%) familial. Twelve (63.2%) were females. Developmental epileptic encephalopathy (DEE) was observed in 18 (94.7%) patients and ID alone in 1 (5.3%) individual. Thirteen patients (68.4%) had profound ID/GDD, 4 (23.53%) severe, 1 (5.9%) moderate and 1 (5.9%) mild. Three patients (15.8%) with profound ID died. A total of 19 variants were detected: pathogenic (n = 15) and likely pathogenic (n = 4). Seven were novel variants: c.664-1G>-, M486R, H245N, H498Pfs*44, L41R, L410del, and D90H. Of the 8 previous reported variants, 2 were recurrent: R406C and R292C. Anti-seizure medications were used in combinations, and 7 patients became seizure-free, and most of them achieved seizure freedom within the first 2 years of life irrespective of the type of the mutation. Effective medications for the seizure-free individuals included adrenocorticotropic (ACTH) and/or levetiracetam and/or phenobarbital and/or sodium valproate and/or topiramate and/or vigabatrin and/or nitrazepam. There was no correlation between the types of pathogenic variants and the phenotypes.

Conclusion

Our case-series showed that there is no genotype-phenotype correlation in patients with STXBP1-related disorders. This study adds 7 novel variants which expand the spectrum of STXBP1-related disorders. Combinations of levetiracetam and/or sodium valproate and/or ACTH and/or phenobarbital and/or vigabatrin and/or topiramate and/or nitrazepam were more often associated with seizure freedom in our cohort within 2 years of life.
Maternal dietary components in the development of gestational diabetes mellitus: a systematic review of observational studies to timely promotion of health
Authors: Lambert Victoria , Muñoz Sonia Edith , Gil Carla , Román María Dolores
Published: March 2023

Background

There is ample evidence that considers diet as an important factor in the prevention of gestational diabetes mellitus (GDM). The aim of this review is to synthesise the existing evidence on the relationship between GDM and maternal dietary components.

Methods

We performed a systematic bibliographic search in Medline, Latin American and Caribbean Health Sciences Literature (Lilacs) and the Latin American Nutrition Archive (ALAN) of regional and local literature, limiting the searches to observational studies published between 2016 and 2022. Search terms related to nutrients, foods, dietary patterns and the relationship to GDM risk were used. The review included 44 articles, 12 of which were from America. The articles considered different topics about maternal dietary components as follows: 14 are about nutrient intake, 8 about food intake, 4 combined nutrient and food analysis and 18 about dietary patterns.

Results

Iron, processed meat and a low carbohydrate diet were positively associated with GDM. Antioxidant nutrients, folic acid, fruits, vegetables, legumes and eggs were negatively associated with GDM. Generally, western dietary patterns increase GDM risk, and prudent dietary patterns or plant-based diets decrease the risk.

Conclusions

Diet is considered one of the causes of GDM. However, there is no homogeneity in how people eat nor in how researchers assess diet in different contextual conditions of the world.
The epigenetic regulatory mechanism of PIWI/piRNAs in human cancers
Authors: Zhang Qun , Zhu Yazhi , Cao Xinyu , Tan Wenhui , Yu Jianglong , Lu Yaqiong , Kang Ran , Wang Xiaolan , Li Ermao
Published: March 2023

PIWI proteins have a strong correlation with PIWI-interacting RNAs (piRNAs), which are significant in development and reproduction of organisms. Recently, emerging evidences have indicated that apart from the reproductive function, PIWI/piRNAs with abnormal expression, also involve greatly in varieties of human cancers. Moreover, human PIWI proteins are usually expressed only in germ cells and hardly in somatic cells, so the abnormal expression of PIWI proteins in different types of cancer offer a promising opportunity for precision medicine. In this review, we discussed current researches about the biogenesis of piRNA, its epigenetic regulatory mechanisms in human cancers, such as N6-methyladenosine (m6A) methylation, histone modifications, DNA methylation and RNA interference, providing novel insights into the markers for clinical diagnosis, treatment and prognosis in human cancers.
Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat study
Authors: Kniha Kristian , Buhl Eva Miriam , Al-Sibai Faruk , Möhlhenrich Stephan Christian , Bock Anna , Heitzer Marius , Hölzle Frank , Modabber Ali
Published: March 2023

Background

This rat study aimed to evaluate the feasibility of temperature thresholds that affect peri-implant bone cells and morphology and the potential usefulness of thermal necrosis for inducing implant removal for a subsequent in vivo pig study.

Methods

On one side, rat tibiae were thermally treated before implant insertion. The contralateral side was used as the control group without tempering. Temperatures of 4 °C, 3 °C, 2 °C, 48 °C, 49 °C, and 50 °C were evaluated with a tempering time of 1 min. Energy-dispersive X-ray spectroscopy (EDX) and transmission electron microscopy (TEM) analyses were performed.

Results

The EDX analysis revealed significant increases in element weights at 50 °C (e.g., calcium, phosphate, sodium, and sulfur; p < 0.01). The results of the TEM analysis showed that at all the applied cold and warm temperatures, signs of cell damage were observed, including vacuolization, shrinkage, and detachment from the surrounding bone matrix. Some cells became necrotic, leaving the lacunae empty.

Conclusions

Temperature of 50 °C led to irreversible cell death. The degree of damage was more significant at 50 °C and 2 °C than at 48 °C and 5 °C. Although this was a preliminary study, from the results, we identified that a temperature of 50 °C at a time interval of 60 min can lower the number of samples in a further study of thermo-explantation. Thus, the subsequent planned in vivo study in pigs, which will consider osseointegrated implants, is feasible.
Sexual dimorphism in odontometric parameters using cone beam CT: a systematic review
Authors: Ajmal M. A. , Roberts Tina S. , Beshtawi Khaled R. , Raj A. C. , Sandeepa N. C.
Published: March 2023

Objective

To determine whether odontometric parameters using cone beam computed tomography (CBCT) would aid in sex estimation by assessing sexual dimorphism of odontometric parameters.

Material and methods

The focused question was whether there is sexual dimorphism in linear and volumetric odontometric parameters when assessed using CBCT. The preferred reporting items for systematic reviews and meta-analysis (PRISMA) guidelines were followed to conduct a systematic search until June 2022 in all major databases. Data were extracted regarding the population, size of the sample, age range, teeth analyzed, linear or volumetric measurements, accuracy, and conclusion. The quality of included studies was assessed using (Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool.

Results

Out of the 3761 studies identified, twenty-nine full-text articles were assessed for eligibility. Finally, twenty-three articles (4215 participants) that provided data on odontometrics using CBCT were included in this systematic review. The odontological sex estimation were assessed either linear measurements (n = 13) or volumetric measurements (n = 8) or both (n = 2). Canines were analysed in maximum number of reports (n = 14), followed by incisors (n = 11), molars(n = 10) and premolars(n = 6). Most of the reports (n = 18) confirmed the existence of sexual dimorphism in odontometric parameters when assessed using CBCT. No significant differences in odontometrics between the sexes were noted in some reports (n = 5). The accuracy of sex estimation was assessed in eight investigations, which ranged from 47.8 to 92.3%.

Conclusions

Odontometrics of human permanent dentition using CBCT exhibit a certain degree of sexual dimorphism. Both linear and volumetric measurements of teeth can aid sex estimation.
Network regulatory mechanism of ncRNA on the Wnt signaling pathway in osteoporosis
Authors: An Fangyu , Meng Xiangrui , Yuan Lingqing , Niu Yanqiang , Deng Jie , Li Zhaohui , Liu Yongqi , Xia Ruoliu , Liu Shiqing , Yan Chunlu
Published: March 2023

Non-coding RNA (ncRNA) is a type of non-protein-coding RNA molecule transcribed from the genome which performs broad regulation of a variety of biological functions in human cells. The Wnt signaling pathway is highly conserved in multicellular organisms, playing an important role in their growth and development. Increasing evidence suggests that ncRNA can regulate cell biological function, enhance bone metabolism, and maintain normal bone homeostasis by interacting with the Wnt pathway. Studies have also demonstrated that the association of ncRNA with the Wnt pathway may be a potential biomarker for the diagnosis, evaluation of prognosis, and treatment of osteoporosis. The interaction of ncRNA with Wnt also performs an important regulatory role in the occurrence and development of osteoporosis. Targeted therapy of the ncRNA/Wnt axis may ultimately be the preferred choice for the treatment of osteoporosis in the future. The current article reviews the mechanism of the ncRNA/Wnt axis in osteoporosis and reveals the relationship between ncRNA and Wnt, thereby exploring novel molecular targets for the treatment of osteoporosis and providing theoretical scientific guidance for its clinical treatment.
Elevated serum immunoglobulin level predicts high risk of 1-year recurrence in patients with Takayasu arteritis
Authors: Guo Yanqiu , Du Juan , Li Taotao , Gao Na , Yang Shiyu , Zhang Yaxin , Pan Lili
Published: March 2023

Background

The mechanism of humoral immunity to Takayasu arteritis (TAK) is not clear. In our study, we aimed to investigate the correlation between immunoglobulins and disease activity and the relationship between immunoglobulins and the prognosis of TAK patients.

Methods

One hundred ninety TAK patients divided into two groups according to whether they had elevated immunoglobulins or not. We compared the demographic data and the clinical data between the two groups. Pearson correlation was used to analyze the relationship between immunoglobulin and disease activity, as well as the relationship between their changes. Immunohistochemical staining was used to compare the expression of humoral immune cells in TAK and atherosclerotic patients. One hundred twenty TAK patients who achieved remission within 3 months after discharge were followed up for 1 year. Logistic regression was used to explore the relationship between elevated immunoglobulins and recurrence.

Results

Disease activity and inflammatory factors were significantly higher in the group with elevated immunoglobulins than in the normal group [NIH (3.0 vs. 2.0, P = 0.001), ITAS-A (9.0 vs. 7.0, P = 0.006)]. Compared with atherosclerotic patients, CD138 + plasma cells were significantly increased in the aortic wall of patients with TAK (P = 0.021). Changes in IgG correlated well with CRP and ESR [CRP (r = 0.40, P = 0.027), ESR (r = 0.64, P < 0.001)]. For patients with TAK in remission, elevated immunoglobulins was associated with 1-year recurrence [OR95%, CI: 2.37 (1.03, 5.47), P = 0.042].

Conclusions

Immunoglobulins is of clinical value in evaluating disease activity in TAK patients. Moreover, the dynamic changes of IgG were correlated with the changes in inflammatory indicators in TAK patients.
The double homeodomain protein DUX4c is associated with regenerating muscle fibers and RNA-binding proteins
Authors: Claus Clothilde , Slavin Moriya , Ansseau Eugénie , Lancelot Céline , Bah Karimatou , Lassche Saskia , Fiévet Manon , Greco Anna , Tomaiuolo Sara , Tassin Alexandra , Dudome Virginie , Kusters Benno , Declèves Anne-Emilie , Laoudj-Chenivesse Dalila , van Engelen Baziel G. M. , Nonclercq Denis , Belayew Alexandra , Kalisman Nir , Coppée Frédérique
Published: March 2023

Background

 We have previously demonstrated that double homeobox 4 centromeric (DUX4C) encoded for a functional DUX4c protein upregulated in dystrophic skeletal muscles. Based on gain- and loss-of-function studies we have proposed DUX4c involvement in muscle regeneration. Here, we provide further evidence for such a role in skeletal muscles from patients affected with facioscapulohumeral muscular dystrophy (FSHD).

Methods

DUX4c was studied at RNA and protein levels in FSHD muscle cell cultures and biopsies. Its protein partners were co-purified and identified by mass spectrometry. Endogenous DUX4c was detected in FSHD muscle sections with either its partners or regeneration markers using co-immunofluorescence or in situ proximity ligation assay.

Results

We identified new alternatively spliced DUX4C transcripts and confirmed DUX4c immunodetection in rare FSHD muscle cells in primary culture. DUX4c was detected in nuclei, cytoplasm or at cell–cell contacts between myocytes and interacted sporadically with specific RNA-binding proteins involved, a.o., in muscle differentiation, repair, and mass maintenance.

In FSHD muscle sections, DUX4c was found in fibers with unusual shape or central/delocalized nuclei (a regeneration feature) staining for developmental myosin heavy chain, MYOD or presenting intense desmin labeling. Some couples of myocytes/fibers locally exhibited peripheral DUX4c-positive areas that were very close to each other, but in distinct cells. MYOD or intense desmin staining at these locations suggested an imminent muscle cell fusion.

We further demonstrated DUX4c interaction with its major protein partner, C1qBP, inside myocytes/myofibers that presented features of regeneration. On adjacent muscle sections, we could unexpectedly detect DUX4 (the FSHD causal protein) and its interaction with C1qBP in fusing myocytes/fibers.

Conclusions

DUX4c upregulation in FSHD muscles suggests it contributes not only to the pathology but also, based on its protein partners and specific markers, to attempts at muscle regeneration. The presence of both DUX4 and DUX4c in regenerating FSHD muscle cells suggests DUX4 could compete with normal DUX4c functions, thus explaining why skeletal muscle is particularly sensitive to DUX4 toxicity. Caution should be exerted with therapeutic agents aiming for DUX4 suppression because they might also repress the highly similar DUX4c and interfere with its physiological role.

Graphical Abstract
Macrophage-derived exosomes mediate glomerular endothelial cell dysfunction in sepsis-associated acute kidney injury
Authors: Xiang Huiling , Xu Zhifeng , Zhang Chun , Xiong Jing
Published: March 2023

Background

Sepsis-associated AKI has been shown to be related to sepsis mortality. Macrophage activation and endothelial cell damage are involved in the progression of sepsis-associated AKI, but the specific mechanisms are still unclear.

Methods

In vitro experiments, exosomes extracted from lipopolysaccharide (LPS) -stimulated macrophages were co-incubated with rat glomerular endothelial cells (RGECs) and then detected the injury markers of RGECs. Acid sphingomyelinase (ASM) inhibitor amitriptyline were used to investigate the role of ASM. In vivo experiment, exosomes produced by LPS-stimulated macrophages were injected into mice through tail vein to further explore the role of macrophage-derived exosomes. Moreover, ASM knockout mice were used to verify the mechanism.

Result

In vitro, the secretion of macrophage exosomes increased upon the stimulation with LPS. Notably, macrophage-derived exosomes can cause glomerular endothelial cell dysfunction. In vivo, macrophage infiltration and exosome secretion in glomeruli of the LPS-induced AKI group increased. The exosomes produced by LPS-stimulated macrophages were injected into mice, which also led to the injury of renal endothelial cells. In addition, in the LPS-induced AKI mouse model, compared with wild-type mice, the secretion of exosomes in glomeruli of ASM gene knockout mice and the injury of endothelial cells were reduced.

Conclusion

Our study shows that ASM regulates the secretion of macrophage exosomes, leading to endothelial cell injury, which may be a therapeutic target in sepsis-associated AKI.
Firm growth, exporting and information communication technology (ICT) in Southern Africa
Authors: Masenyetse Rethabile , Manamathela Malefu
Published: March 2023

This paper examines the key determinants of firm growth in three Southern African countries of Eswatini, Lesotho and Namibia and assesses whether exporting activity and use of information communication technology play any role in growth of the firms. The paper uses data collected by the World Bank through enterprise surveys during the period 2014–2016. Employing the ordinary least squares, the results show that firm growth is determined by size of the firm, age, use of information communication technology, exporting activities, foreign ownership and the legal form of the firm. The result is robust even when controlling for industry and country differences. Overall, the results support industrial policy geared towards small firms through rolling out information communication technology infrastructure and promoting access to exporting markets.
The role of the environment in entrepreneurial propensity of youngsters’ business ideas
Authors: Czakó Katalin , Polster Csilla , Setyaningsih Santi , Vasic Tihana
Published: March 2023

Entrepreneurship shows regional differences through countries’ history, economic environments, society and habits of individuals. Youngsters can think globally and have the highest skills to access information and know-how. This paper collects data from students’ project work from different countries and extract that information, which can give interesting input to describe regional effects on entrepreneurial propensity through a quantified semantic analysis. Youngsters from 12 countries received the same instructions to present their national economies and then create a business there. The fundamental question of the paper is that are there any definable attributes from the economic environment, which effect youngsters’ entrepreneurial propensity? How can we describe these effects? We linked values and made a numerical evaluation of youngsters’ business concepts, which gives not only a range but also the grade of the presence of local or regional elements. The results show that the more exact special elements of a national economy mentioned, the more barriers participants are aware of. The research concept and the results may give useful inputs in knowledge transfer and education of entrepreneurship.
p53 directly downregulates the expression of CDC20 to exert anti-tumor activity in mantle cell lymphoma
Authors: Chen Yingtong , Yang Ping , Wang Jing , Gao Shuang , Xiao Shiyu , Zhang Weilong , Zhu Mingxia , Wang Yanfang , Ke Xiaoyan , Jing Hongmei
Published: March 2023

Background

Cell cycle dysregulation characterized by cyclin D1 overexpression is common in mantle cell lymphoma (MCL), while mitotic disorder was less studied. Cell division cycle 20 homologue (CDC20), an essential mitotic regulator, was highly expressed in various tumors. Another common abnormality in MCL is p53 inactivation. Little was known about the role of CDC20 in MCL tumorigenesis and the regulatory relationship between p53 and CDC20 in MCL.

Methods

CDC20 expression was detected in MCL patients and MCL cell lines harboring mutant p53 (Jeko and Mino cells) and wild-type p53 (Z138 and JVM2 cells). Z138 and JVM2 cells were treated with CDC20 inhibitor apcin, p53 agonist nutlin-3a, or in combination, and then cell proliferation, cell apoptosis, cell cycle, cell migration and invasion were determined by CCK-8, flow cytometry and Transwell assays. The regulatory mechanism between p53 and CDC20 was revealed by dual-luciferase reporter gene assay and CUT&Tag technology. The anti-tumor effect, safety and tolerability of nutlin-3a and apcin were investigated in vivo in the Z138-driven xenograft tumor model.

Results

CDC20 was overexpressed in MCL patients and cell lines compared with their respective controls. The typical immunohistochemical marker of MCL patients, cyclin D1, was positively correlated with CDC20 expression. CDC20 high expression indicated unfavorable clinicopathological features and poor prognosis in MCL patients. In Z138 and JVM2 cells, either apcin or nutlin-3a treatment could inhibit cell proliferation, migration and invasion, and induce cell apoptosis and cell cycle arrest. GEO analysis, RT-qPCR and WB results showed that p53 expression was negatively correlated with CDC20 expression in MCL patients, Z138 and JVM2 cells, while this relationship was not observed in p53-mutant cells. Dual-luciferase reporter gene assay and CUT&Tag assay revealed mechanistically that CDC20 was transcriptionally repressed by p53 through directly binding p53 to CDC20 promoter from − 492 to + 101 bp. Moreover, combined treatment of nutlin-3a and apcin showed better anti-tumor effect than single treatment in Z138 and JVM2 cells. Administration of nutlin-3a/apcin alone or in combination confirmed their efficacy and safety in tumor-bearing mice.

Conclusions

Our study validates the essential role of p53 and CDC20 in MCL tumorigenesis, and provides a new insight for MCL therapeutics through dual-targeting p53 and CDC20.
Endogenous small intestinal microbiome determinants of transient colonisation efficiency by bacteria from fermented dairy products: a randomised controlled trial
Authors: Zaccaria Edoardo , Klaassen Tim , Alleleyn Annick M. E. , Boekhorst Jos , Smokvina Tamara , Kleerebezem Michiel , Troost Freddy J.
Published: March 2023

Background

The effects of fermented food consumption on the small intestine microbiome and its role on host homeostasis are largely uncharacterised as our knowledge on intestinal microbiota relies mainly on faecal samples analysis. We investigated changes in small intestinal microbial composition and functionality, short chain fatty acid (SCFA) profiles, and on gastro-intestinal (GI) permeability in ileostomy subjects upon the consumption of fermented milk products.

Results

We report the results from a randomised, cross-over, explorative study where 16 ileostomy subjects underwent 3, 2-week intervention periods. In each period, they consumed either milk fermented by Lacticaseibacillus rhamnosus CNCM I-3690, or milk fermented by Streptococcus thermophilus CNCM I-1630 and Lactobacillus delbrueckii subsp. bulgaricus CNCM I-1519, or a chemically acidified milk (placebo) daily. We performed metataxonomic, metatranscriptomic analysis, and SCFA profiling of ileostomy effluents as well as a sugar permeability test to investigate the microbiome impact of these interventions and their potential effect on mucosal barrier function. Consumption of the intervention products impacted the overall small intestinal microbiome composition and functionality, mainly due to the introduction of the product-derived bacteria that reach in several samples 50% of the total microbial community. The interventions did not affect the SCFA levels in ileostoma effluent, or gastro-intestinal permeability and the effects on the endogenous microbial community were negligible. The impact on microbiome composition was highly personalised, and we identified the poorly characterised bacterial family, Peptostreptococcaceae, to be positively associated with a low abundance of the ingested bacteria. Activity profiling of the microbiota revealed that carbon- versus amino acid-derived energy metabolism of the endogenous microbiome could be responsible for the individual-specific intervention effects on the small intestine microbiome composition and function, reflected also on urine microbial metabolites generated through proteolytic fermentation.

Conclusions

The ingested bacteria are the main drivers of the intervention effect on the small intestinal microbiota composition. Their transient abundance level is highly personalised and influenced by the energy metabolism of the ecosystem that is reflected by its microbial composition (http://www.clinicaltrials.gov, ID NCT NCT02920294).
miR-34a/DRP-1-mediated mitophagy participated in cisplatin-induced ototoxicity via increasing oxidative stress
Authors: Wang Haiyan , Lin Hanqing , Kang Weibiao , Huang Lingfei , Gong Sisi , Zhang Tao , Huang Xiaotong , He Feinan , Ye Yongyi , Tang Yiyang , Jia Haiying , Yang Haidi
Published: March 2023

Purpose

Cisplatin is a widely used and effective chemotherapeutic agent for most solid malignant tumors. However, cisplatin-induced ototoxicity is a common adverse effect that limits the therapeutic efficacy of tumors in the clinic. To date, the specific mechanism of ototoxicity has not been fully elucidated, and the management of cisplatin-induced ototoxicity is also an urgent challenge. Recently, some authors believed that miR34a and mitophagy played a role in age-related and drug-induced hearing loss. Our study aimed to explore the involvement of miR-34a/DRP-1-mediated mitophagy in cisplatin-induced ototoxicity.

Methods

In this study, C57BL/6 mice and HEI-OC1 cells were treated with cisplatin. MiR-34a and DRP-1 levels were analyzed by qRT‒PCR and western blotting, and mitochondrial function was assessed via oxidative stress, JC-1 and ATP content. Subsequently, we detected DRP-1 levels and observed mitochondrial function by modulating miR-34a expression in HEI-OC1 cells to determine the effect of miR-34a on DRP-1-mediated mitophagy.

Results

MiR-34a expression increased and DRP-1 levels decreased in C57BL/6 mice and HEI-OC1 cells treated with cisplatin, and mitochondrial dysfunction was involved in this process. Furthermore, the miR-34a mimic decreased DRP-1 expression, enhanced cisplatin-induced ototoxicity and aggravated mitochondrial dysfunction. We further verified that the miR-34a inhibitor increased DRP-1 expression, partially protected against cisplatin-induced ototoxicity and improved mitochondrial function.

Conclusion

MiR-34a/DRP-1-mediated mitophagy was related to cisplatin-induced ototoxicity and might be a novel target for investigating the treatment and protection of cisplatin-induced ototoxicity.
Co-enrichment of CD8-positive T cells and macrophages is associated with clinical benefit of tislelizumab in solid tumors
Authors: Ye Dingwei , Desai Jayesh , Shi Jingwen , Liu Si-Yang Maggie , Shen Wei , Liu Tengfei , Shi Yang , Wang Dan , Liang Liang , Yang Silu , Ma Xiaopeng , Jin Wei , Zhang Pei , Huang Ruiqi , Shen Zhirong , Zhang Yun , Wu Yi-Long
Published: March 2023

Background

Activated immune cells (IC) in the tumor microenvironment (TME) are critical for anti-tumor efficacy. Greater understanding of the dynamic diversity and crosstalk between IC is needed to clarify their association with immune checkpoint inhibitor efficacy.

Methods

Patients from three tislelizumab monotherapy trials in solid tumors (NCT02407990, NCT04068519, NCT04004221) were retrospectively divided into subgroups by CD8+ T-cell and macrophage (Mφ) levels, assessed via multiplex immunohistochemistry (mIHC; n = 67) or gene expression profiling (GEP; n = 629).

Results

A trend of longer survival was observed in patients with both high CD8+ T-cell and Mφ levels versus other subgroups in the mIHC analysis (P = 0.11), which was confirmed with greater statistical significance in the GEP analysis (P = 0.0001). Co-existence of CD8+ T cells and Mφ was coupled with elevated CD8+ T-cell cytotoxicity, T-cell trafficking, MHC class I antigen presentation signatures/genes, and enrichment of the pro-inflammatory Mφ polarization pathway. Additionally, a high level of pro-inflammatory CD64+ Mφ density was associated with an immune-activated TME and survival benefit with tislelizumab (15.2 vs. 5.9 months for low density; P = 0.042). Spatial proximity analysis revealed that closer proximity between CD8+ T cells and CD64+ Mφ was associated with a survival benefit with tislelizumab (15.2 vs. 5.3 months for low proximity; P = 0.024).

Conclusions

These findings support the potential role of crosstalk between pro-inflammatory Mφ and cytotoxic T cells in the clinical benefit of tislelizumab.

Trial registration

NCT02407990, NCT04068519, NCT04004221.
Influence of prey availability on habitat selection during the non-breeding period in a resident bird of prey
Authors: Bühler Roman , Schalcher Kim , Séchaud Robin , Michler Stephanie , Apolloni Nadine , Roulin Alexandre , Almasi Bettina
Published: March 2023

Background

For resident birds of prey in the temperate zone, the cold non-breeding period can have strong impacts on survival and reproduction with implications for population dynamics. Therefore, the non-breeding period should receive the same attention as other parts of the annual life cycle. Birds of prey in intensively managed agricultural areas are repeatedly confronted with unpredictable, rapid changes in their habitat due to agricultural practices such as mowing, harvesting, and ploughing. Such a dynamic landscape likely affects prey distribution and availability and may even result in changes in habitat selection of the predator throughout the annual cycle.

Methods

In the present study, we (1) quantified barn owl prey availability in different habitats across the annual cycle, (2) quantified the size and location of barn owl breeding and non-breeding home ranges using GPS-data, (3) assessed habitat selection in relation to prey availability during the non-breeding period, and (4) discussed differences in habitat selection during the non-breeding period to habitat selection during the breeding period.

Results

The patchier prey distribution during the non-breeding period compared to the breeding period led to habitat selection towards grassland during the non-breeding period. The size of barn owl home ranges during breeding and non-breeding were similar, but there was a small shift in home range location which was more pronounced in females than males. The changes in prey availability led to a mainly grassland-oriented habitat selection during the non-breeding period. Further, our results showed the importance of biodiversity promotion areas and undisturbed field margins within the intensively managed agricultural landscape.

Conclusions

We showed that different prey availability in habitat categories can lead to changes in habitat preference between the breeding and the non-breeding period. Given these results we show how important it is to maintain and enhance structural diversity in intensive agricultural landscapes, to effectively protect birds of prey specialised on small mammals.
Micro fading test for textile single yarns: a new methodology applied to the Reformation Tapestry to assess its sensitivity to light
Authors: Vannucci Giulia , Joram Salwa , Beselin Anna , Röhrs Stefan
Published: March 2023

Tapestries and especially pile carpets can be challenging objects for assessing their photosensitivity by microfading: they are often made of relatively thick yarns resulting in a non-flat airy surface. For this reason, focusing the light on the object’s surface is difficult and the precision of the colour change measurement is hampered. In this study, an improved test methodology was developed to overcome the difficulties by analysing single yarns. The methodology consists in flattening the yarn between glass microscope slides tightly held together by means of 4 strong paired neodymium magnets. The results show that the newly-developed sample preparation together with the required instrumental adjustment allow to significantly reduce the standard deviation and variation coefficient which often characterize microfading measurements of textiles. The methodology for sample preparation is evaluated and had been applied to samples from the Reformation Tapestry dating to 1667 from the collection of the Museum Europäischer Kulturen (Museum of European Cultures) in Berlin.
Stock identification of Congaturi halfbeak (Hyporhamphus limbatus): insight into conventional and truss-based morphometrics
Authors: Mahfuj Sarower , Islam Sk Injamamul , Jinia Sharmin Sultana , Hossain Md. Firoj , Atique Usman
Published: March 2023

Background

Wild fish stocks continuously decline in Bangladeshi rivers and oxbow lakes. Small indigenous fish species management solely depends on their population status and conservation. This study aimed to determine the population status of Hyporhamphus limbatus, in Southwestern Bangladesh using conventional and truss morphological characteristics.

Results

A total of 174 fish samples were procured from monsoon season i.e., May to August 2018 the Bhairab River, BR; Kopotakkho River, KR; and Baluhar Baor, BB in Bangladesh. Five meristics, six conventional and twelve truss-based morphometric characters, were considered for stock identification. The Kruskal–Wallis test helped to analyze the meristic characters, while ANOVA, principal component analysis (PCA), discriminant function analysis (DFA), correct classification analysis using DFA, and a UPGMA dendrogram formation were used to investigate the conventional and truss measurements. By the Kruskal–Wallis test, no meristic characters showed significant differences across different populations. Eleven of the 18 morphometric measurements showed significant differences among three populations through the univariate ANOVA. PCA specified the population structure variations and explained 67.438% of the total variance. The first and second discrimination functions accounted for 78.3% and 21.7%, representing 100% of the group variability. Similarly, 85.6% of the grouped cases and 77.6% of cross-validated grouped cases were initially effectively represented by their correct number of individuals. A dendrogram based on morphometric (conventional and truss) displayed three stocks grouped into two clusters, with BR forming a distinct cluster, while KR and BB creating a shared cluster.

Conclusion

In conclusion, such morphological differences are most likely due to their distinctive ancestral origins. This study presents novel reports on the stock assessment of H. limbatus in their natural ecosystem. Furthermore, molecular research and an evaluation of the environmental impact on H. limbatus populations in Bangladesh are strongly recommended.
Extrinsic arterial compression and lower extremity ischemia after iliac vein stent placement: case report, review of literature
Authors: Elsayed Mohammad , Sarkar Debkumar , Zhao Ken , Bryce Yolanda , Friedman Adie
Published: March 2023

Background

Lower extremity ischemia due to extrinsic arterial compression by venous stent placement is a rare but increasingly recognized occurrence. Given the rise of complex venous interventions, awareness of this entity is becoming increasingly important to avoid serious complications.

Case Presentation

A 26-year-old with progressively enlarging pelvic sarcoma despite chemoradiation developed recurrent symptomatic right lower extremity deep venous thrombosis due to worsening mass effect on a previously placed right common iliac vein stent. This was treated with thrombectomy and stent revision, with extension of the right common iliac vein stent to the external iliac vein. During the immediate post-procedure period the patient developed symptoms of acute right lower extremity arterial ischemia including diminished pulses, pain, and motorsensory loss. Imaging confirmed extrinsic compression of the external iliac artery by the newly placed adjacent venous stent. The patient underwent stenting of the compressed artery with complete resolution of ischemic symptoms.

Conclusions

Awareness and early recognition of arterial ischemia following venous stent placement is important to prevent serious complication. Potential risk factors include patients with active pelvis malignancy, prior radiation, or scarring from surgery or other inflammatory processes. In cases of threatened limb, prompt treatment with arterial stenting is recommended. Further study is warranted to optimize detection and management of this complication.
A case–control study of single-nucleotide variants in microRNA biogenesis genes (AGO1 and GEMIN4) in people with primary immune thrombocytopenia
Authors: Mohamed Haydi Sayed , Abdelghany Wafaa Mohamed , Eid Inas Abdelmoaty Mohamed
Published: March 2023

Background

The role of microRNA (miRNA) is to regulate the translation of genes involved in a variety of diseases.

Aim of the work

This study investigated the relation between the rs636832 and rs2740348 single-nucleotide polymorphisms (SNPs) of the AGO1 gene and the GEMIN4 gene of miRNA biogenesis genes and the risk for primary ITP and the response to therapy.

Patients and methods

In this case–control study, 100 patients with immune thrombocytopenic purpura from the clinical hematology department and outpatient clinic were compared to 100 control subjects. Patients' blood samples are taken, and DNA extraction and PCR amplification of rs636832 A/G of AGO1 and rs2740348 G/C of GEMIN4 were done on sera and compared to the clinical status of the patients.

Results

According to statistical analysis, the genotype and allele frequencies of both variants did not differ significantly between cases and controls. The GG genotype was found to be more prevalent in the chronic phase of ITP than the AA and AG genotypes, with a significance level of p 0.05. The GG genotype was found to be more prevalent in the chronic phase of ITP than the AA and AG genotypes, with a significance level of p 0.05. In addition, the GG genotype was more prevalent in ITP patients who did not respond to treatment compared to the AA and AG genotypes, although this difference was not statistically significant. Concerning rs2740348: the CC genotype was more common than the GC genotype in ITP cases that did not respond to treatment and needed a second line of therapy like splenectomy or TPO-RA, but this did not reach statistical significance.

Conclusion

The rs636832 and rs2740348 SNPs did not appear to be risk factors for ITP; however, the rs636832:GG genotype was more prevalent in older patients, who tend to have the chronic phase of the disease. Although this distinction was not statistically significant, they were less responsive to therapy than the AA and AG genotypes. Concerning rs2740348: the CC genotype was more common than the GC genotype in ITP cases that did not respond to treatment and needed a second line of therapy like splenectomy or TPO-RA, but this did not reach statistical significance.