bluuebunny/crossref_metadata_2025 huggingface dataset:
Gap Crossing in Flying Squirrels: Mitigating Movement Barriers through Landscape Management and Structural Implementation
Authors: Howard Jeremy M.
Published: November 2022

Habitat fragmentation affects flying squirrels despite their ability to cross canopy gaps. If unable to cross gaps, flying squirrels may suffer from limited access to appropriate resources, inbreeding depression, and even extirpation. North American flying squirrels (Glaucomys) have been the focus of limited research on this issue when compared to other areas of the world tackling this problem. However, as all gliding mammals share similar conservation challenges, findings of other species on other continents can be applied to the Glaucomys species in North America. The purpose of this review is to take a metapopulation approach to the problem of gap crossing. This review first discusses necessary habitat conservation strategies for Glaucomys within the patches they reside. The review then discusses patch size and configuration, honing in on maintaining connectivity between habitat patches. Different structures (natural and manmade) used to maintain connectivity are reviewed using gliding mammal literature from around the world. This information is pertinent to North American conservation ecologists and landscape managers, who can use this information to improve habitat connectivity and facilitate crossings of Glaucomys flying squirrels within metapopulations.
Valorization of Local Legumes and Nuts as Key Components of the Mediterranean Diet
Authors: Hernández-López Israel , Ortiz-Solà Jordi , Alamprese Cristina , Barros Lillian , Shelef Oren , Basheer Loai , Rivera Ana , Abadias Maribel , Aguiló-Aguayo Ingrid
Published: November 2022

Legumes and nuts are components of high importance in the diet of many countries, mainly those in the Mediterranean region. They are also very versatile and culturally diverse foods found all over the world, acting as a basic protein source in certain countries. Their genetic diversity is needed to sustain the food supply and security for humans and livestock, especially because of the current loss of habitats, species, and genetic diversity worldwide, but also because of the ever present need to feed the increasing human population. Even though both legumes and nuts are considered as high-protein food and environmentally friendly crops, developed countries have lower consumption rates when compared to Asia or Africa. With a view to increasing the consumption of legumes and nuts, the objective of this review is to present the advantages on the use of autochthonous varieties from different countries around the world, thus providing a boost to the local market in the area. The consumption of these varieties could be helped by their use in ready-to-eat foods (RTE), which are now on the rise thanks to today’s fast-paced lifestyles and the search for more nutritious and sustainable foods. The versatility of legumes and nuts covers a wide range of possibilities through their use in plant-based dairy analogues, providing alternative-protein and maximal amounts of nutrients and bioactive compounds, potential plant-based flours for bakery and pasta, and added-value traditional RTE meals. For this reason, information about legume and nut nutrition could possibly increase its acceptance with consumers.
Bergamot Essential Oil: A Method for Introducing It in Solid Dosage Forms
Authors: Zambito Ylenia , Piras Anna Maria , Fabiano Angela
Published: November 2022

Bergamot essential oil (BEO) possess antimicrobial, antiproliferative, anti-inflammatory, analgesic, neuroprotective, and cardiovascular effects. However, it is rich in volatile compounds, e.g., limonene, that are susceptible to conversion and degradation reactions. The aim of this communication was to prepare a conjugate based on a quaternary ammonium chitosan derivative (QA-Ch) and methyl-βCD (MCD), coded as BEO/QA-Ch-MCD, to encapsulate BEO in order to stabilize its volatile compounds, eliminate its unpleasant taste, and convert the oil in a solid dosage form. The obtained conjugate, BEO/QA-Ch-MCD, was highly soluble and had a percentage of extract association efficiency (AE %), in terms of polyphenols and limonene contents, of 22.0 ± 0.9 and 21.9 ± 1.2, respectively. Moreover, stability studies under UV stress in simulated gastric fluid showed that BEO/QA-Ch-MCD was more able to protect polyphenols and limonene from degradation compared to free BEO or BEO complexed with MCD (BEO/MCD). The complexation and subsequent lyophilization allowed the transformation of a liquid into a solid dosage form capable of eliminating the unpleasant taste of the orally administered oil and rendering the solid suitable to produce powders, granules, tablets, etc. These solid oral dosage forms, as they come into contact with physiological fluids, could generate nanosized agglomerates able to increase the stability of their active contents and, consequently, their bioavailability.
Application of Lactose-Free Whey Protein to Greek Yogurts: Potential Health Benefits and Impact on Rheological Aspects and Sensory Attributes
Authors: da Silva Agatha Transfeld , de Lima Jair José , Reis Priscila , Passos Maurício , Baumgartner Catiucia Giraldi , Sereno Aiane Benevide , Krüger Cláudia Carneiro Hecke , Cândido Lys Mary Bileski
Published: November 2022

The application of β-galactosidase in the fermentation of milk enables the acquirement of lower levels of lactose that are tolerated by lactose maldigesters and can reduce the nutritional consequences of avoiding dairy products. The present study evaluated the viability of the fortification of lactose-free prebiotic Greek yogurt formulas with whey protein concentrate (WPC). Two rotational central composite designs (RCCDs) were applied: one to perform the hydrolysis of the whey protein concentrate and another for the yogurt formulations (α = 2 with 2 central points and 4 axial points). Two β-galactosidase enzymes obtained from Kluyveromyces lactis were used. The content of lactose, glucose, galactose, and lactic acid were determined in the WPC, milk (pasteurized and powdered), and yogurts. The three best formulations regarding the attributes’ viscosity, syneresis, firmness, and elasticity were sensorially evaluated by using a nine-point hedonic scale. A microbiological analysis was performed after 48 h of yogurt production. The characterization of the products and the comparison of the results obtained were evaluated using the Student’s T test and the analysis of variance with Tukey’s test (p-values < 0.05). The application of a lactose-free WPC promoted viscosity, firmness, and elasticity. The syneresis was reduced, and whey increased the protein and calcium content. Lactose-free WPC can be used as a partial substitute for skimmed powdered milk in yogurts. The obtained results are encouraging with respect to the production of lactose-free Greek yogurts by the dairy industry.
Insights into Protective Effects of Different Synbiotic Microcapsules on the Survival of Lactiplantibacillus plantarum by Electrospraying
Authors: Li Shu-Fang , Feng Kun , Huang Ru-Meng , Wei Yun-Shan , Wu Hong
Published: November 2022

This study evaluated the protective effects of different synbiotic microcapsules on the viability of encapsulated Lactiplantibacillus plantarum GIM1.648 fabricated by electrospraying. The optimum amount of substrate for three synbiotic microcapsules separately containing fructooligosaccharide (FOS), fish oil, and the complex of both were 4% FOS (SPI-F-L-P), 20 μL fish oil (SPI-O-L-P) and the complex of 20 μL fish oil, and 2% FOS (SPI-O-F-L-P), respectively. The obtained synbiotic microcapsules had a better encapsulation efficiency (EE) and survival rate (SR) after in vitro digestion than microcapsules without the addition of substrate (SPI-L-P) and SPI-O-F-L-P presented the highest EE (95.9%) and SR (95.5%). When compared to SPI-L-P, the synbiotic microcapsules possessed a more compact structure as proved by the SEM observation and their cell viability were significantly improved in response to environmental stresses (heat treatment, freeze drying, and storage). The synbiotic microcapsules containing the complex of FOS and fish oil showed the best beneficial effect, followed by ones with fish oil and then FOS, suggesting the FOS and fish oil complex has more potential in application.
Performing a Sonar Acceptance Test of the Kongsberg EM712 Using Open-Source Software: A Case Study of Kluster
Authors: Younkin Eric , Umfress S. Harper
Published: November 2022

In the world of seafloor mapping, the ability to explore and experiment with a dataset in its raw and processed forms is critical. Kluster is an open-source multibeam data processing software package written in Python that enables this exploration. Kluster provides a suite of multibeam processing features, including analysis, visualization, gridding, and data cleaning. We demonstrated these features using a recently acquired dataset from a Kongsberg EM712 multibeam echosounder aboard NOAA Ship Fairweather. This test dataset served to illustrate the fundamental analysis abilities of the software, as well as its utility as a troubleshooting tool both in the field and during post-processing. Kluster has the capability to perform the Sonar Acceptance Test in full, including common experiments like the patch test, extinction test, and accuracy test, which are generally performed on new systems. When questions arise regarding the integration or parameter settings of a system, this software allows the user to quickly and clearly visualize much of the raw data and its associated metadata, which is a vital step in any investigative effort. With its emphasis on accessibility and ease of use, Kluster is an excellent tool for users who are inexperienced with multibeam sonar data processing.
Clinical and Psychological Variables in Female Patients with Cervical Syndromes: A Cross-Sectional and Correlational Study
Authors: Cabanillas-Barea Sara , Carrasco-Uribarren Andoni , Medrano-de-la-Fuente Ricardo , Jiménez-del-Barrio Sandra , Pardos-Aguilella Pilar , Pérez-Guillén Silvia , Ceballos-Laita Luis
Published: November 2022

Background: The objectives of this study were: (1) to compare the pain intensity, cervical range of motion (ROM), psychological distress and kinesiophobia in patients with cervicogenic dizziness (CGD), tension-type headache (TTH), and mechanical chronic neck pain (MCNP); and (2) to investigate the relationships between pain intensity and cervical ROM and between psychological distress and kinesiophobia. Methods: a cross-sectional and correlational study was designed. In total, 109 patients (32 patients with CGD, 33 with TTH and 44 with MCNP) were included. Pain intensity, cervical ROM, psychological distress and kinesiophobia were assessed. Results: Statistically significant differences were found between the groups in pain intensity, psychological distress and kinesiophobia. The patients with MCNP showed higher pain intensity compared to the other groups (p < 0.001). The patients with CGD showed higher depression and kinesiophobia values compared to the MCNP and TTH groups (p < 0.05). No differences were found for cervical flexion, extension, lateral flexion, or rotation ROM (p > 0.05). The CGD and MCNP groups found a moderate positive correlation between psychological distress and kinesiophobia (p < 0.05). The patients with TTH and MCNP showed a moderate positive correlation between pain intensity, psychological distress and kinesiophobia (p < 0.05). Conclusion: Pain intensity, psychological distress and kinesiophobia should be considered in the three groups. Psychological distress was correlated with kinesiophobia in the CGD and MCNP groups. The MCNP group showed a correlation between pain intensity, psychological distress and kinesiophobia.
College Students’ eMental Health Literacy and Risk of Diagnosis with Mental Health Disorders
Authors: Cormier Eileen , Park Hyejin , Schluck Glenna
Published: November 2022

Background: This study investigated college students’ eMental health literacy (eMHL), knowledge of common mental disorders and risk of being diagnosed with a mental health disorder and compared their knowledge of mental disorders and concurrent risk of diagnosis with high and low eMHL; Method: A total of 123 college students completed an online survey through Amazon’s Mechanical Turk (MTurk). Data were analyzed using descriptive statistics and chi-square tests; Results: eMental health literacy scores were higher when students had a history of prior mental health problems, were female, and graduate students. College students with high eMHL were more likely to recognize symptoms, recommend professional help, and be at lower risk for common mental health disorders compared to low eMHL students; Conclusions: eMHL was associated with mental health status and demographic variables. Level of eMHL was associated with knowledge of mental disorders and risk of diagnosis. Implications: The results highlight the need for targeted interventions to enhance eMHL of college students, support mental health resilience and prevent mental health disorders.
Increased Imports of Colorants and Constituent Components during the 18th Century Reflects the Start of the Consumer Society in Norway
Authors: Knudtzon Margaret Aasness
Published: November 2022

The start of the consumer society in Norway is examined by studying the increased imports of colorants and their constituents during the 18th century. Based on historical customs records, 82 imported pigments and dyes, 27 binders and additives and nine mordants and auxiliaries are presented. Imports increased significantly in the middle and at end of the century, representing two chromatic “revolutions”. This was especially evident for lead white and indigo; being the only particularly white and blue pigments used for painting and dyeing, respectively. Red dyes at different prices and properties (brazilwood, madder and cochineal) met the demands for red textile coloring in different social groups. The study presents a comprehensive overview of colorant imports and provides new insights in the development of consumption in Norway. Colorant imports were probably initiated by a supply-driven positive feed-back loop as a result of increased export trade. This was followed by a demand-driven loop, involving increased domestic trade, product preferences, “fashionability”, consumer culture, economic conditions and enlightenment. A model is presented that can contribute to a further understanding of the start of the consumer society in the second half of the 18th century in Norway.
What Is Happening in the Squares of China? Exploring the Experience of Participating in Square Sports and Sustainability Factors
Authors: Li Guoyi , Lee Jungrae
Published: November 2022

This phenomenological study explored the experience of participation in square sports in China and the social mechanisms by which they can be sustained. Ten study participants were selected through a purposeful sampling method. The findings indicate that their physical and mental health were either maintained or improved as they engaged in square sports. They also experienced reduced feelings of loneliness and an increase in their sense of belonging through exchanges with other members of their teams. They enjoyed the freedom from cost and spatial restrictions in pursuing leisure activities. However, conflicts also arose with other groups, mainly related to securing space in the squares. Additionally, the study found that conflicts between participants and non-participants in square sports emerged as a social problem. The social mechanisms by which square sports can be sustained were identified as people-led voluntary participation, pride in square sports, and the reproduction of economic capital using human resources.
Convolutional Neural Network Classification of Rest EEG Signals among People with Epilepsy, Psychogenic Non Epileptic Seizures and Control Subjects
Authors: Lo Giudice Michele , Ferlazzo Edoardo , Mammone Nadia , Gasparini Sara , Cianci Vittoria , Pascarella Angelo , Mammì Anna , Mandic Danilo , Morabito Francesco Carlo , Aguglia Umberto
Published: November 2022

Identifying subjects with epileptic seizures or psychogenic non-epileptic seizures from healthy subjects via interictal EEG analysis can be a very challenging issue. Indeed, at visual inspection, EEG can be normal in both cases. This paper proposes an automatic diagnosis approach based on deep learning to differentiate three classes: subjects with epileptic seizures (ES), subjects with non-epileptic psychogenic seizures (PNES) and control subjects (CS), analyzed by non-invasive low-density interictal scalp EEG recordings. The EEGs of 42 patients with new-onset ES, 42 patients with PNES video recorded and 19 patients with CS all with normal interictal EEG on visual inspection, were analyzed in the study; none of them was taking psychotropic drugs before registration. The processing pipeline applies empirical mode decomposition (EMD) to 5s EEG segments of 19 channels in order to extract enhanced features learned automatically from the customized convolutional neural network (CNN). The resulting CNN has been shown to perform well during classification, with an accuracy of 85.7%; these results encourage the use of deep processing systems to assist clinicians in difficult clinical settings.
Sports Bra Pressure: Effect on Body Skin Temperature and Wear Comfort
Authors: Yick Kit-Lun , Keung Yin-Ching , Yu Annie , Wong Kam-Ho , Hui Kwok-Tung , Yip Joanne
Published: November 2022

Sports bras are an essential apparel for active women, but may exert excessive pressure that negatively affects thermoregulation, thermal comfort and wear sensation. This study measures skin temperature changes during short durations of exercise on a treadmill with different bra pressures. The results based on 21 female subjects (age: 27.2 ± 4.5 years old) show that bras with more pressure at the underband or shoulder straps do not cause statistically significant skin temperature changes during exercise (p > 0.05). Nevertheless, compared to the optimal bra fit, significant differences in bra-breast skin temperature are found during running, cooling down and sitting when the bra pressure is increased (p < 0.05), particularly under bra cup (T1) in this study. The FLIR thermal images can visualize the skin temperature changes at abdomen throughout the four activity stages. Subjective sensations of bra thermal comfort, pressure and breast support are assessed. Despite the increased pressure on the shoulders and chest wall, perceptions towards thermal comfort remain unchanged. The perceived pressure comfort and support sensation amongst the 4 bra conditions are comparable. Interestingly, positive sensations of pressure comfort and breast support are perceived with a tight-fitting sports bra during treadmill exercise. High pressures induced by sports bras (>4 kPa) that habitually considered harmful to the human body may not lead to wear discomfort but enhance bra support sensation and a sense of security to the wearers.
How and for Whom Is Mobile Phone Addiction Associated with Mind Wandering: The Mediating Role of Fatigue and Moderating Role of Rumination
Authors: Lian Shuailei , Bai Xuqing , Zhu Xiaowei , Sun Xiaojun , Zhou Zongkui
Published: November 2022

With the increasing prevalence of mobile phone addiction, mobile phone addiction has been considered a prominent risk factor for internalizing or externalizing problems, such as psychological distress and irrational procrastination. However, few studies shed light on the effect of mobile phone addiction on mind wandering and the underlying mechanisms. This study speculated that the direct effect of mobile phone addiction on mind wandering may be linked to fatigue and that the level of an individual’s personality characteristics, such as rumination, may influence both the direct and indirect effects of mobile phone addiction on mind wandering. To test these hypotheses, we recruited 1811 college students to complete the self-report questionnaires. The results indicated that mobile phone addiction was positively associated with mind wandering. This direct effect could be mediated by fatigue, and both the direct and indirect effects of mobile phone addiction on mind wandering could be moderated by rumination. Specifically, both the direct and indirect effects were stronger for students with high rumination. These findings enrich our understanding of how, why, and for whom mobile phone addiction is correlated with mind wandering.
Facilitators and Barriers of Artificial Intelligence Applications in Rehabilitation: A Mixed-Method Approach
Authors: Alsobhi Mashael , Sachdev Harpreet Singh , Chevidikunnan Mohamed Faisal , Basuodan Reem , K U Dhanesh Kumar , Khan Fayaz
Published: November 2022

Artificial intelligence (AI) has been used in physical therapy diagnosis and management for various impairments. Physical therapists (PTs) need to be able to utilize the latest innovative treatment techniques to improve the quality of care. The study aimed to describe PTs’ views on AI and investigate multiple factors as indicators of AI knowledge, attitude, and adoption among PTs. Moreover, the study aimed to identify the barriers to using AI in rehabilitation. Two hundred and thirty-six PTs participated voluntarily in the study. A concurrent mixed-method design was used to document PTs’ opinions regarding AI deployment in rehabilitation. A self-administered survey consisting of several aspects, including demographic, knowledge, uses, advantages, impacts, and barriers limiting AI utilization in rehabilitation, was used. A total of 63.3% of PTs reported that they had not experienced any kind of AI applications at work. The major factors predicting a higher level of AI knowledge among PTs were being a non-academic worker (OR = 1.77 [95% CI; 1.01 to 3.12], p = 0.04), being a senior PT (OR = 2.44, [95%CI: 1.40 to 4.22], p = 0.002), and having a Master/Doctorate degree (OR = 1.97, [95%CI: 1.11 to 3.50], p = 0.02). However, the cost and resources of AI were the major reported barriers to adopting AI-based technologies. The study highlighted a remarkable dearth of AI knowledge among PTs. AI and advanced knowledge in technology need to be urgently transferred to PTs.
“COVID Is Another Layer of Problematic Things”: Change, Vulnerability, and COVID-19 among University Students
Authors: David Ifeolu , Schatz Enid , Myroniuk Tyler W. , Teti Michelle
Published: November 2022

The COVID-19 pandemic not only had detrimental effects on physical health but also had adverse effects on college students’ mental health. This paper begins to fill a gap in knowledge related to the contextual factors that impacted college students’ mental health during COVID. Using in-depth interviews with a diverse sample of 33 college students at a Midwestern university, during Spring 2021, we highlight the pandemic’s role in shaping college students’ mental health and their outlook of the future. Thematic analysis revealed student reports of mental health decline during the pandemic attributed to campus closures and social distancing policies implemented by the institution to reduce the spread of COVID-19. Students shared that the pandemic created uncertainties about their future opportunities for education, career fulfillment, and employment. However, the interviews also suggested a general sense of adaptation to the pandemic’s impact which was students achieved via a combination of active and passive coping strategies. Expanding institution-based mental health services to include a variety of modalities and off-line toolkits for students can help students cope with mental health challenges, whether in ‘normal times’ or during national crises. Future research should focus on identifying strategies for promoting mental wellness among college students and exploring post-pandemic mental health wellbeing.
Spatiotemporal Changes and Driving Factors of Ecosystem Health in the Qinling-Daba Mountains
Authors: Xiang Ting , Meng Xiaoliang , Wang Xinshuang , Xiong Jing , Xu Zelin
Published: November 2022

Rapid industrialization and urbanization have accelerated land-use changes in mountainous areas, with dramatic impacts on ecosystem health. In particular, the Qinling-Daba Mountains, as China’s central water tower, ecological green lung, and biological gene bank, have rich resource endowments and extremely high ecological value and are an important protective wall to China’s ecological security. Therefore, understanding the level of ecosystem health and its drivers in the research area contributes to the conservation and restoration of the mountain ecosystem. Based on remote sensing image data and land-use data from 2000 to 2020, we explored the spatial characteristics of ecosystem health, and supplemented with socio-economic data to explore its driving factors. The results show that (1) the ecosystem health in the study area has been continuously improved during the study period, and the regional differences in ecological organization are the most prominent; (2) the level of ecosystem health in the Qinling-Daba Mountains has been spatially improved from the peripheral areas to the central area, showing significant spatial autocorrelation and local spatial aggregation; (3) the ecosystem health is influenced by a combination of natural and anthropogenic factors, among which the negative effect of GRDP is mainly concentrated in the eastern region, the negative effect of the proportion of built-up land gradually spreads to the western region, and the positive effect of the proportion of forest land has a large scale. This study contributes to a better understanding of ecosystem health in mountainous counties in China and provides useful information for policymakers to formulate ecological and environmental management policies.
2-O-β-d-Glucopyranosyl-4,6-dihydroxybenzaldehyde Isolated from Morus alba (Mulberry) Fruits Suppresses Damage by Regulating Oxidative and Inflammatory Responses in TNF-α-Induced Human Dermal Fibroblasts
Authors: Kim Kang Sub , Choi Yea Jung , Jang Dae Sik , Lee Sullim
Published: November 2022

Human skin is composed of three layers, of which the dermis is composed of an extracellular matrix (ECM) comprising collagen, elastin, and other proteins. These proteins are reduced due to skin aging caused by intrinsic and extrinsic factors. Among various internal and external factors related to aging, ultraviolet (UV) radiation is the main cause of photoaging of the skin. UV radiation stimulates DNA damage, reactive oxygen species (ROS) generation, and pro-inflammatory cytokine production such as tumor necrosis factor-alpha (TNF-α), and promotes ECM degradation. Stimulation with ROS and TNF-α upregulates mitogen-activated protein kinases (MAPKs), nuclear factor kappa B (NF-κB), and activator protein 1 (AP-1) transcription factors that induce the expression of the collagenase matrix metalloproteinase-1 (MMP-1). Moreover, TNF-α induces intracellular ROS production and several molecular pathways. Skin aging progresses through various processes and can be prevented through ROS generation and TNF-α inhibition. In our previous study, 2-O-β-d-glucopyranosyl-4,6-dihydroxybenzaldehyde (GDHBA) was isolated from the Morus alba (mulberry) fruits and its inhibitory effect on MMP-1 secretion was revealed. In this study, we focused on the effect of GDHBA on TNF-α-induced human dermal fibroblasts (HDFs). GDHBA (50 μM) inhibited ROS generation (18.8%) and decreased NO (58.4%) and PGE2 levels (53.8%), significantly. Moreover, it decreased MMP-1 secretion (55.3%) and increased pro-collagen type I secretion (207.7%). GDHBA (50 μM) decreased the expression of different MAPKs as per western blotting; p-38: 35.9%; ERK: 47.9%; JNK: 49.5%; c-Jun: 32.1%; NF-κB: 55.9%; and cyclooxygenase-2 (COX-2): 31%. This study elucidated a novel role of GDHBA in protecting against skin inflammation and damage through external stimuli, such as UV radiation.
Deletion of POMT2 in Zebrafish Causes Degeneration of Photoreceptors
Authors: Liu Yu , Rittershaus Jaclyn M. , Yu Miao , Sager Rachel , Hu Huaiyu
Published: November 2022

Mutations in the extracellular matrix protein eyes shut homolog (EYS) are a common cause of retinitis pigmentosa, a blinding disease characterized by photoreceptor degeneration. EYS binds to matriglycan, a carbohydrate modification on O-mannosyl glycan substitutions of the cell-surface glycoprotein α-dystroglycan. Patients with mutations in enzymes required for the biosynthesis of matriglycan exhibit syndromic retinal atrophy, along with brain malformations and congenital muscular dystrophy. Protein O-mannosyltransferase 2 (POMT2) is an enzyme required for the synthesis of O-mannosyl glycans. To evaluate the roles of O-mannosyl glycans in photoreceptor health, we generated protein O-mannosyltransferase 2 (pomt2) mutant zebrafish by CRISPR. pomt2 mutation resulted in a loss of matriglycan and abolished binding of EYS protein to α-dystroglycan. Mutant zebrafish presented with hydrocephalus and hypoplasia of the cerebellum, as well as muscular dystrophy. EYS protein was enriched near photoreceptor connecting cilia in the wild-type, but its presence and proper localization was significantly reduced in mutant animals. The mutant retina exhibited mis-localization of opsins and increased apoptosis in both rod and cone photoreceptors. Immunofluorescence intensity of G protein subunit alpha transducin 2 (GNAT2) antibody (a general cone marker) and 1D4 antibody (a long double cone marker) in mutant retinas did not differ from wild-type retinas at 1-month post fertilization, but was reduced at 6 months post fertilization, indicating significant cone degeneration. These data suggest that POMT2-mediated O-mannosyl glycosylation is required for EYS protein localization to the connecting cilium region and photoreceptor survival.
The STIM1/2-Regulated Calcium Homeostasis Is Impaired in Hippocampal Neurons of the 5xFAD Mouse Model of Alzheimer’s Disease
Authors: Skobeleva Ksenia , Shalygin Alexey , Mikhaylova Elena , Guzhova Irina , Ryazantseva Maria , Kaznacheyeva Elena
Published: November 2022

Alzheimer’s disease (AD) is the most common cause of age-related dementia. Neuronal calcium homeostasis impairment may contribute to AD. Here we demonstrated that voltage-gated calcium (VGC) entry and store-operated calcium (SOC) entry regulated by calcium sensors of intracellular calcium stores STIM proteins are affected in hippocampal neurons of the 5xFAD transgenic mouse model. We observed excessive SOC entry in 5xFAD mouse neurons, mediated by STIM1 and STIM2 proteins with increased STIM1 contribution. There were no significant changes in cytoplasmic calcium level, endoplasmic reticulum (ER) bulk calcium levels, or expression levels of STIM1 or STIM2 proteins. The potent inhibitor BTP-2 and the FDA-approved drug leflunomide reduced SOC entry in 5xFAD neurons. In turn, excessive voltage-gated calcium entry was sensitive to the inhibitor of L-type calcium channels nifedipine but not to the T-type channels inhibitor ML218. Interestingly, the depolarization-induced calcium entry mediated by VGC channels in 5xFAD neurons was dependent on STIM2 but not STIM1 protein in cells with replete Ca2+ stores. The result gives new evidence on the VGC channel modulation by STIM2. Overall, the data demonstrate the changes in calcium signaling of hippocampal neurons of the AD mouse model, which precede amyloid plaque accumulation or other signs of pathology manifestation.
3-Dimensional Immunostaining and Automated Deep-Learning Based Analysis of Nerve Degeneration
Authors: Drake Sienna S. , Charabati Marc , Simas Tristan , Xu Yu Kang T. , Maes Etienne J. P. , Shi Shan Shan , Antel Jack , Prat Alexandre , Morquette Barbara , Fournier Alyson E.
Published: November 2022

Multiple sclerosis (MS) is an autoimmune and neurodegenerative disease driven by inflammation and demyelination in the brain, spinal cord, and optic nerve. Optic neuritis, characterized by inflammation and demyelination of the optic nerve, is a symptom in many patients with MS. The optic nerve is the highway for visual information transmitted from the retina to the brain. It contains axons from the retinal ganglion cells (RGCs) that reside in the retina, myelin forming oligodendrocytes and resident microglia and astrocytes. Inflammation, demyelination, and axonal degeneration are also present in the optic nerve of mice subjected to experimental autoimmune encephalomyelitis (EAE), a preclinical mouse model of MS. Monitoring the optic nerve in EAE is a useful strategy to study the presentation and progression of pathology in the visual system; however, current approaches have relied on sectioning, staining and manual quantification. Further, information regarding the spatial load of lesions and inflammation is dependent on the area of sectioning. To better characterize cellular pathology in the EAE model, we employed a tissue clearing and 3D immunolabelling and imaging protocol to observe patterns of immune cell infiltration and activation throughout the optic nerve. Increased density of TOPRO staining for nuclei captured immune cell infiltration and Iba1 immunostaining was employed to monitor microglia and macrophages. Axonal degeneration was monitored by neurofilament immunolabelling to reveal axonal swellings throughout the optic nerve. In parallel, we developed a convolutional neural network with a UNet architecture (CNN-UNet) called BlebNet for automated identification and quantification of axonal swellings in whole mount optic nerves. Together this constitutes a toolkit for 3-dimensional immunostaining to monitor general optic nerve pathology and fast automated quantification of axonal defects that could also be adapted to monitor axonal degeneration and inflammation in other neurodegenerative disease models.
Pseudorogneria libanotica Intraspecific Genetic Polymorphism Revealed by Fluorescence In Situ Hybridization with Newly Identified Tandem Repeats and Wheat Single-Copy Gene Probes
Authors: Wu Dandan , Yang Namei , Xiang Qian , Zhu Mingkun , Fang Zhongyan , Zheng Wen , Lu Jiale , Sha Lina , Fan Xing , Cheng Yiran , Wang Yi , Kang Houyang , Zhang Haiqin , Zhou Yonghong
Published: November 2022

The genus Pseudoroegneria (Nevski) Löve (Triticeae, Poaceae) with its genome abbreviated ‘St’ accounts for more than 60% of perennial Triticeae species. The diploid species Psudoroegneria libanotica (2n = 14) contains the most ancient St genome. Therefore, investigating its chromosomes could provide some fundamental information required for subsequent studies of St genome evolution. Here, 24 wheat cDNA probes covering seven chromosome groups were mapped in P. libanotica to distinguish homoelogous chromosomes, and newly identified tandem repeats were performed to differentiate seven chromosome pairs. Using these probes, we investigated intraspecific population chromosomal polymorphism of P. libanotica. We found that (i) a duplicated fragment of the 5St long arm was inserted into the short arm of 2St; (ii) asymmetrical fluorescence in situ hybridization (FISH) hybridization signals among 2St, 5St, and 7St homologous chromosome pairs; and (iii) intraspecific population of polymorphism in P. libanotica. These observations established the integrated molecular karyotype of P. libanotica. Moreover, we suggested heterozygosity due to outcrossing habit and adaptation to the local climate of P. libanotica. Specifically, the generated STlib_96 and STlib_98 repeats showed no cross-hybridization signals with wheat chromosomes, suggesting that they are valuable for identifying alien chromosomes or introgressed fragments of wild relatives in wheat.
Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds
Authors: Azueta-Aguayo Paola Hassibe , Chuc-Gamboa Martha Gabriela , Aguilar-Pérez Fernando Javier , Aguilar-Ayala Fernando Javier , Rodas-Junco Beatriz A. , Vargas-Coronado Rossana Faride , Cauich-Rodríguez Juan Valerio
Published: November 2022

It has been reported that chitosan scaffolds, due to their physicochemical properties, stimulate cell proliferation in different tissues of the human body. This study aimed to determine the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide, with different pH values, to better understand cell behavior depending on the pH of the biomaterial. Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing. In vitro cytotoxicity was assessed via dental-pulp stem cells’ (DPSCs’) biocompatibility. The results revealed that the neutralized scaffolds exhibited better cell proliferation and morphology. It was concluded that the chitosan scaffolds’ high pH (due to residual ammonium hydroxide) decreases DPSCs’ cell viability.
BMP/Smad Pathway Is Involved in Lithium Carbonate-Induced Neural-Tube Defects in Mice and Neural Stem Cells
Authors: Yang Aiyun , Li Shen , Zhang Yan , Wang Xiuwei , Guan Zhen , Zhu Zhiqiang , Liang Yingchao , Zhao Lijiao , Wang Jianhua
Published: November 2022

Neural-tube defects (NTDs) are one type of the most serious birth defects. Studies have shown that inositol deficiency is closely related to the occurrence of NTDs. Bone morphogenetic protein (BMP)-mediated Smad signaling pathways have been implicated in neurogenesis and neural-tube closure. However, the role of the BMP/Smad pathway in inositol-deficiency-induced NTDs remains unclear. Inositol-deficiency models in C57 mice and mouse neural stem cells (mNSCs) were induced with Li2CO3 treatment or inositol withdrawal. The role of the BMP/Smad pathway in the regulation of cell proliferation and the development of NTDs was determined utilizing qRT-PCR, HE staining, Western blot, immunostaining, MTT assay, EdU staining, and flow cytometry. The intraperitoneal injection of Li2CO3 at Embryonic Day 7.5 induced the occurrence of NTDs. The mRNA levels of Bmp2, Bmp4, Smad1, Smad5, Smad8 and Runx2, the phosphorylation of Smad1/5/8, and the nuclear translocation of Runx2 were significantly increased in NTD embryonic brain tissues and mNSCs exposed to Li2CO3 or an inositol-free medium, which were suppressed by BMP receptor selective inhibitor LDN-193189. The Li2CO3-induced phosphorylation of Smad1/5/8 was inhibited by inositol supplementation. Cell proliferation was significantly promoted by Li2CO3 exposure or the absence of inositol in mNSCs, which was reversed by LDN-193189. These results suggest that the activation of the BMP/Smad signaling pathway might play an important role in the development of NTDs induced by maternal Li2CO3 exposure via inositol deficiency.
Different Effects of Different Lactobacillus acidophilus Strains on DSS-Induced Colitis
Authors: Huang Zheng , Gong Lei , Jin Yan , Stanton Catherine , Ross Reynolds Paul , Zhao Jianxin , Yang Bo , Chen Wei
Published: November 2022

Inflammatory bowel disease (IBD) is a worldwide chronic intestinal inflammatory immune-related disease. In this study, mice with dextran sulfate sodium (DSS)-induced colitis were used to evaluate the effect of Lactobacillus acidophilus on colitis. The results revealed that L. acidophilus CCFM137 and FAHWH11L56 show potential for relieving colitis symptoms, while L. acidophilus FGSYC48L79 did not show a protective effect. Moreover, L. acidophilus NCFM and FAHWH11L56 showed similar effects on various indicators of DSS-induced colitis, increasing the IL-10 and IL-17 in the colon, and modifying the CCL2/CCR2 axis and CCL3/CCR1 axis. For L. acidophilus CCFM137, its effects on colitis were different from the above two strains. Moreover, L. acidophilus FGSYC48L79 had negative effects on colitis by increasing the abundance of harmful bacteria in the gut microbiota and may promote the signaling of chemokines and their receptors. This may be related to its special genome compared to the other strains.
Screening and Molecular Mechanisms of Novel ACE-Inhibitory Peptides from Gracilariopsis lemaneiformis
Authors: Su Yongchang , Chen Shicheng , Shen Jiashen , Yi Zhiwei , Liu Shuji , Cai Shuilin , Pan Nan , Qiao Kun , Chen Xiaoting , Chen Bei , Xu Min , Yang Suping , Liu Zhiyu
Published: November 2022

Candidate peptides with novel angiotensin-I-converting enzyme (ACE) inhibitor activity were obtained from hydrolysates of Gracilariopsis lemaneiformis by virtual screening method. Our results showed that G. lemaneiformis peptides (GLP) could significantly lower blood pressure in spontaneously hypertensive rats (SHR). At least 101 peptide sequences of GLP were identified by LC-MS/MS analysis and subjected to virtual screening. A total of 20 peptides with the highest docking score were selected and chemically synthesized in order to verify their ACE-inhibitory activities. Among them, SFYYGK, RLVPVPY, and YIGNNPAKG showed good effects with IC50 values of 6.45 ± 0.22, 9.18 ± 0.42, and 11.23 ± 0.23 µmoL/L, respectively. Molecular docking studies revealed that three peptides interacted with the active center of ACE by hydrogen bonding, hydrophobic interactions, and electrostatic forces. These peptides could form stable complexes with ACE. Furthermore, SFYYGK, RLVPVPY, and YIGNNPAKG significantly reduced systolic blood pressure (SBP) in SHR. YIGNNPAKG exhibited the highest antihypertensive effect, with the largest decrease in SBP (approximately 23 mmHg). In conclusion, SFYYGK, RLVPVPY, and YIGNNPAKG can function as potent therapeutic candidates for hypertension treatment.
Increased Prevalence of Unstable HLA-C Variants in HIV-1 Rapid-Progressor Patients
Authors: Stefani Chiara , Sangalli Antonella , Locatelli Elena , Federico Tania , Malerba Giovanni , Romanelli Maria Grazia , Argañaraz Gustavo Adolfo , Da Silva Bosco Christiano Maciel , Da Silva Alberto Jose Duarte , Casseb Jorge , Argañaraz Enrique Roberto , Ruggiero Alessandra , Zipeto Donato
Published: November 2022

HIV-1 infection in the absence of treatment results in progression toward AIDS. Host genetic factors play a role in HIV-1 pathogenesis, but complete knowledge is not yet available. Since less-expressed HLA-C variants are associated with poor HIV-1 control and unstable HLA-C variants are associated with higher HIV-1 infectivity, we investigated whether there was a correlation between the different stages of HIV-1 progression and the presence of specific HLA-C allotypes. HLA-C genotyping was performed using allele-specific PCR by analyzing a treatment-naïve cohort of 96 HIV-1-infected patients from multicentric cohorts in the USA, Canada, and Brazil. HIV-1-positive subjects were classified according to their different disease progression status as progressors (Ps, n = 48), long-term non-progressors (LTNPs, n = 37), and elite controllers (ECs, n = 11). HLA-C variants were classified as stable or unstable according to their binding stability to β2-microglobulin/peptide complex. Our results showed a significant correlation between rapid progression to AIDS and the presence of two or one unstable HLA-C variants (p-value: 0.0078, p-value: 0.0143, respectively). These findings strongly suggest a link between unstable HLA-C variants both at genotype and at allele levels and rapid progression to AIDS. This work provides further insights into the impact of host genetic factors on AIDS progression.
Fine Mapping of BoVl Conferring the Variegated Leaf in Ornamental Kale (Brassica oleracea var. acephala)
Authors: Ren Jie , Zou Jiaqi , Zou Xiao , Song Gengxing , Gong Zhichao , Liu Zhiyong , Ji Ruiqin , Feng Hui
Published: November 2022

Ornamental kale, as a burgeoning landscaping plant, is gaining popularity for its rich color patterns in leaf and cold tolerance. Leaf variegation endows ornamental kale with unique ornamental characters, and the mutants are ideal materials for exploring the formation mechanisms of variegated phenotype. Herein, we identified a novel variegated leaf kale mutant ‘JC007-2B’ with green margins and white centers. Morphological observations and physiological determinations of the green leaf stage (S1), albino stage (S2) and variegated leaf stage (S3) demonstrated that the chloroplast structure and photosynthetic pigment content in the white sectors (S3_C) of variegated leaves were abnormal. Genetic analysis revealed that a single dominant nuclear gene (BoVl) controlled the variegated leaf trait of ‘JC007-2B’, and three candidate genes for BoVl were fine-mapped to a 6.74 Kb interval on chromosome C03. Multiple sequence alignment among the green-leaf mapping parent ‘BS’, recombinant individuals, mutant parent ‘JC007-2B’ and its same originated DH line population established that the mutation sites in Bo3g002080 exhibited a complete consensus. Bo3g002080, homologous to Arabidopsis MED4, was identified as the candidate gene for BoVl. Expression analysis showed that Bo3g002080 displayed a 2158.85-fold higher expression at albino stage than that in green leaf stage. Transcriptome analysis showed that related pathways of photosynthesis and chloroplast development were significantly enriched in the white sectors, and relevant DEGs involved in these pathways were almost down-regulated. Overall, our study provides a new gene resource for cultivar breeding in ornamental kale and contributes to uncovering the molecular genetic mechanism underlying the variegated leaf formation.
Impact of Caterpillar Increased Feeding Rates on Reduction of Bt Susceptibility
Authors: Dhammi Anirudh , van Krestchmar Jaap B. , Zhu Jiwei , Ponnusamy Loganathan , Gould Fred , Reisig Dominic , Kurtz Ryan W. , Roe R. Michael
Published: November 2022

The use of insect-resistant transgenic crops producing Bacillus thuringiensis protein Cry toxins (Bt) to control caterpillars is wide-spread. Development of a mechanism to prevent Bt from reaching its target site in the digestive system could result in Bt resistance and resistance to other insecticides active per os. Increased feeding rates by increasing temperature in tobacco budworms, Chloridea virescens, and bollworms, Helicoverpa zea, decreased Bt Cry1Ac susceptibility and mortality. The same was found in C. virescens for Bollgard II plant extract containing Bt Cry1Ac and Cry2Ab2 toxins. Furthermore, H. zea from the same inbred laboratory colony that fed faster independent of temperature manipulation were less susceptible to Bt intoxication. A laboratory derived C. virescens Bt resistant strain demonstrated a higher feeding rate on non-Bt artificial diet than the parental, Bt susceptible strain. A laboratory-reared Bt resistant fall armyworm, Spodoptera frugiperda, strain also fed faster on non-Bt diet compared to Bt susceptible caterpillars of the same species, both originally collected from corn. The studies in toto and the literature reviewed support the hypothesis that increased feeding rate is a behavioral mechanism for reducing caterpillar susceptibility to Bt. Its possible role in resistance needs further study.
Design, Synthesis and Actual Applications of the Polymers Containing Acidic P–OH Fragments: Part 1. Polyphosphodiesters
Authors: Nifant’ev Ilya E. , Ivchenko Pavel V.
Published: November 2022

Among natural and synthetic polymers, main-chain phosphorus-containing polyacids (PCPAs) (polyphosphodiesters), stand in a unique position at the intersection of chemistry, physics, biology and medicine. The structural similarity of polyphosphodiesters PCPAs to natural nucleic and teichoic acids, their biocompatibility, mimicking to biomolecules providing the ‘stealth effect’, high bone mineral affinity of polyphosphodiesters resulting in biomineralization at physiological conditions, and adjustable hydrolytic stability of polyphosphodiesters are the basis for various biomedical, industrial and household applications of this type of polymers. In the present review, we discuss the synthesis, properties and actual applications of polyphosphodiesters.