bluuebunny/crossref_metadata_2025 huggingface dataset:
Sustainable Mobility Issues of Physically Active University Students: The Case of Serres, Greece
Authors: Mikiki Foteini , Oikonomou Andreas , Katartzi Ermioni
Published: December 2021

The mobility practices of students are largely dictated by their respective educational obligations. Students of physical education are an ostensibly physically active population, whose active lifestyle may include active travel. University student mobility research calls for behavioral approaches to ground relevant interventions. This work investigated the sustainability practices in the student community of the Physical Education Department in Serres, a medium-sized Greek city. Moreover, this paper aimed to shed light on the gender differences in the physical activity levels of 259 students, as well as their respective differences in mobility practices. A novel questionnaire, based on Ajzen’s theory of planned behavior and Godin–Shephard’s approach to physical activity, was used. The results confirmed higher levels of physical activity in male students, although their attitude toward physical activity was less positive than that of their female classmates. Further positive attitudes in women were recorded toward sustainable mobility choices, although the evidence demonstrated a similar gap between the answers of the two genders. Car possession was higher in men, whereas car purchase intention was slightly lower in women, who had a lower income in general. Moreover, income impacted gender mobility preferences. Recommendations can be guided by students’ sports preferences and can be gender-sensitive, taking income into account.
Effect of ZnO on Properties of Gels for Heritage Objects Conservation
Authors: Mocioiu Oana-Cătălina , Atkinson Irina , Mocioiu Ana-Maria , Neagu Simona , Ruginescu Robert , Mitran Raul-Augustin , Enache Mădălin
Published: December 2021

One of the current research objectives is the development of new films for the conservation of glass heritage objects. The value of historical glass objects is given by the technology and raw materials used in production as well as their transparency and color. Their colors are correlated with oxide composition rich in transitional metals, which decrease resistance of corrosive agents from the atmosphere. In this paper, SiO2-ZnO gels have been designed to protect historical glass objects. The sol–gel method used to obtain gels is a powerful tool for functionalizing different materials. An important functionalization is the antibacterial activity. By applying a gel, the coated material is able to decrease the growth of bacteria. After deposition, some gels must be strengthened by heat treatment. The effect of ZnO content (10 mol% and 20 mol%) on the properties of the studied gels was investigated by Differential scanning calorimetry (DSC), Fourier transform infrared (FTIR), X-ray diffraction (XRD), Scanning electron microscopy (SEM), and antibacterial tests. Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and the halotolerant bacterium, Virgibacillus halodenitrificans, isolated from a salt crystal from Unirea mine, Slănic Prahova, Romania, were used. The gel Gel 2 (SiO2-ZnO (20 mol%)) showed the best properties.
“In the First Place, We Don’t Like to Be Called ‘Refugees’”: Dilemmas of Representation and Transversal Politics in the Participatory Art Project 100% FOREIGN?
Authors: Petersen Anne Ring
Published: December 2021

100% FOREIGN? (100% FREMMED?) is an art project consisting of 250 life stories of individuals who were granted asylum in Denmark between 1956 and 2019. Thus, it can be said to form a collective portrait that inserts citizens of refugee backgrounds into the narrative of the nation, thereby expanding the idea of national identity and culture. 100% FOREIGN? allows us to think of participatory art as a privileged site for the exploration of intersubjective relations and the question of how to “represent” citizens with refugee experience as well as the history and practice of asylum. The conflicting aims and perceptions involved in such representations are many, as suggested by the opening sentence of Hannah Arendt’s 1943 essay “We, Refugees”: “In the first place, we don’t like to be called ‘refugees’”. Using 100% FOREIGN? as an analytical reference point, this article discusses some of the ethical and political implications of representing former refugees. It briefly considers recent Danish immigration and asylum policies to situate the project in its regional European context and argues that, similarly to its neighbouring countries, Denmark can be described as a “postmigrant society” (Foroutan). To frame 100% FOREIGN? theoretically, this article draws on Arendt’s essay, Trinh T. Minh-ha’s concept of speaking nearby, as well as the feminist concept of transversal politics (Meskimmon, Yuval-Davis). It is hoped that this approach will lead to a deeper understanding of what participatory art can bring to the ethical politics of representing refugee experience.
Somatotype Profiles of Montenegrin Karatekas: An Observational Study
Authors: Slankamenac Jelena , Bjelica Dusko , Jaksic Damjan , Trivic Tatjana , Drapsin Miodrag , Vujkov Sandra , Modric Toni , Milosevic Zoran , Drid Patrik
Published: December 2021

Competitive karate activity involves numerous factors affecting performance in sport. Physical structure and somatotype is considered to be one of them. This study aimed to determine whether there are differences between karate athletes in five male and five female official weight categories in different anthropometric measurements and to determine the somatotype profiles of athletes divided by weight categories. This study consisted of a total of 27 male karate athletes (21.88 ± 4.66 years) and 24 female karate athletes (20.29 ± 3.14 years). Measurements were taken in April 2020. Athletes are classified into official weight categories according to World Karate Federation rules. Somatotypes were calculated using anthropometry. One-way analysis of variance and Tukey’s post hoc tests were used for statistical analysis to compare group differences regarding weight categories. Anthropometric parameters were highest in the heaviest categories compared to lighter categories. All male subjects were endomorphic mesomorph, except for category <84 kg, which was endomorphic ectomorphs. Somatotype analysis of male categories found a difference between the <75 kg and <84 kg in endomorphy. In mesomorphy, there is no difference between categories. Perceiving ectomorphy, there is a significant difference between the first category and the >84 kg. Profiling female athletes, three different types of somatotypes were obtained concerning the weight category. The lightest weight category was predominantly endomorphic ectomorphs, and two weight categories were ectomorphic endomorphs (<61 kg and <68 kg), and the other two weight categories were endomorphic mesomorphs (<55 kg and >68 kg). Somatotype differences in the female karate athletes were observed only in the ectomorphy components, between <50 kg and <61 kg. The present study points to how the somatotypes profiles of karate athletes differ between weight categories.
Self-Esteem, Individual versus Team Sports
Authors: Šagát Peter , Bartik Peter , Lazić Anja , Tohănean Dragoș Ioan , Koronas Vasilios , Turcu Ioan , Knjaz Damir , Alexe Cristina Ioana , Curițianu Ioana Maria
Published: December 2021

On the basis of the integrative concept of self-esteem discussed in sport-related literature, various studies refer to its importance in the context of sports activities. Self-esteem is often understood as a personality trait because it tends to be durable and stable. No accurate description is available regarding the types of sports in which subjects participated. The main purpose of the research was to identify and compare the levels of self-esteem and self-confidence of athletes practicing individual and team sports. The self-esteem and self-confidence levels were measured by the Rosenberg Self-Esteem Scale (Rosenberg 1979) and the Self-Confidence Test (Romek, 2000). All participants were males. Subjects were divided into two categories: 40 for individual sports and 40 for team sports. There were two evaluation periods: P1, the beginning of the preparation period, and P2, the beginning of the competition period. There were statistically significant differences for P1 (p < 0.002) and P2 (p < 0.003). The differences between the average values of the two periods were 5.8 points and 3.8 points, both favorable to the group of athletes who practiced individual sports. There were significant differences between the individual and team athletes in self-esteem level. Individual athletes presented a higher level of self-esteem.
Three Novel Bacteria Associated with Two Centric Diatom Species from the Mediterranean Sea, Thalassiosira rotula and Skeletonema marinoi
Authors: Di Costanzo Federica , Di Dato Valeria , van Zyl Leonardo Joaquim , Cutignano Adele , Esposito Francesco , Trindade Marla , Romano Giovanna
Published: December 2021

Diatoms are a successful group of microalgae at the base of the marine food web. For hundreds of millions of years, they have shared common habitats with bacteria, which favored the onset of interactions at different levels, potentially driving the synthesis of biologically active molecules. To unveil their presence, we sequenced the genomes of bacteria associated with the centric diatom Thalassiosira rotula from the Gulf of Naples. Annotation of the metagenome and its analysis allowed the reconstruction of three bacterial genomes that belong to currently undescribed species. Their investigation showed the existence of novel gene clusters coding for new polyketide molecules, antibiotics, antibiotic-resistance genes and an ectoine production pathway. Real-time PCR was used to investigate the association of these bacteria with three different diatom clones and revealed their preference for T. rotula FE80 and Skeletonema marinoi FE7, but not S. marinoi FE60 from the North Adriatic Sea. Additionally, we demonstrate that although all three bacteria could be detected in the culture supernatant (free-living), their number is up to 45 times higher in the cell associated fraction, suggesting a close association between these bacteria and their host. We demonstrate that axenic cultures of T. rotula are unable to grow in medium with low salinity (<28 ppt NaCl) whereas xenic cultures can tolerate up to 40 ppt NaCl with concomitant ectoine production, likely by the associated bacteria.
Investigation of PtSGT1 and PtSGT4 Function in Cellulose Biosynthesis in Populus tomentosa Using CRISPR/Cas9 Technology
Authors: Xue Yinxuan , Li Siyan , Miao Deyu , Huang Sai , Guo Bin , Li Shanwen , An Xin-Min
Published: December 2021

Cellulose synthesis is a complex process in plant cells that is important for wood processing, pulping, and papermaking. Cellulose synthesis begins with the glycosylation of sitosterol by sitosterol glycosyltransferase (SGT) to produce sitosterol-glucoside (SG), which acts as the guiding primer for cellulose production. However, the biological functions of SGTs in Populus tomentosa(P. tomentosa) remain largely unknown. Two full-length PtSGT genes (PtSGT1 and PtSGT4) were previously isolated from P. tomentosa and characterized. In the present study, CRISPR/Cas9 gene-editing technology was used to construct PtSGT1-sgRNA and PtSGT4-sgRNA expression vectors, which were genetically transformed into P. tomentosa using the Agrobacterium-mediated method to obtain transgenic lines. Nucleic acid and amino acid sequencing analysis revealed both base insertions and deletions, in addition to reading frame shifts and early termination of translation in the transgenic lines. Sugar metabolism analysis indicated that sucrose and fructose were significantly downregulated in stems and leaves of mutant PtSGT1-1 and PtSGT4-1. Glucose levels did not change significantly in roots and stems of PtSGT1-1 mutants; however, glucose was significantly upregulated in stems and downregulated in leaves of the PtSGT4-1 mutants. Dissection of the plants revealed disordered and loosely arranged xylem cells in the PtSGT4-1 mutant, which were larger and thinner than those of the wild-type. This work will enhance our understanding of cellulose synthesis in the cell walls of woody plants.
Twenty-Years of Experience in Childhood Glaucoma Surgery
Authors: Wagner Felix Mathias , Schuster Alexander Karl-Georg , Grehn Franz , Urbanek Lukas , Pfeiffer Norbert , Stingl Julia Verena , Hoffmann Esther Maria
Published: December 2021

To quantify the results of childhood glaucoma treatment over time in a cohort of children with different types of childhood glaucoma. A retrospective cohort study of consecutive cases involving children with primary congenital glaucoma, primary juvenile, and secondary juvenile glaucoma at the Childhood Glaucoma Center, University Medical Center Mainz, Germany from 1995 to 2015 was conducted. The main outcome measure was the long-term development of intraocular pressure. Further parameters such as surgical success, refraction, corneal diameter, axial length, and surgical procedure in children with different types of childhood glaucoma were evaluated. Surgical success was defined as IOP < 21 mmHg in eyes without a need for further intervention for pressure reduction. A total of 93 glaucomatous eyes of 61 childhood glaucoma patients with a mean age of 3.7 ± 5.1 years were included. The overall mean intraocular pressure at first visit was 32.8 ± 10.2 mmHg and decreased to 15.5 ± 7.3 mmHg at the last visit. In the median follow-up time of 78.2 months, 271 surgical interventions were performed (130 of these were cyclophotocoagulations). Many (61.9%) of the eyes that underwent surgery achieved complete surgical success without additional medication. Qualified surgical success (with or without additional medication) was reached by 84.5% of the eyes.
Predictors of the Development of Protracted Bacterial Bronchitis following Presentation to Healthcare for an Acute Respiratory Illness with Cough: Analysis of Three Cohort Studies
Authors: O’Grady Kerry-Ann F. , Mahon Juliana , Arnold Daniel , Grimwood Keith , Hall Kerry K. , Goyal Vikas , Marchant Julie M. , Phillips Natalie , Acworth Jason , King Alex , Scott Mark , Chang Anne B.
Published: December 2021

We describe the prevalence and risk factors for protracted bacterial bronchitis (PBB) following healthcare presentation for an acute cough illness in children. Data from three studies of the development of chronic cough (CC) in children were combined. PBB was defined as a wet cough of at least 4-weeks duration with no identified specific cause of cough that resolved following 2–4 weeks of appropriate antibiotics. Anterior nasal swabs were tested for 17 viruses and bacteria by polymerase chain reaction. The study included 903 children. Childcare attendance (adjusted relative risk (aRR) = 2.32, 95% CI 1.48–3.63), prior history of chronic cough (aRR = 2.63, 95% CI 1.72–4.01) and age <2-years (<12-months: aRR = 4.31, 95% CI 1.42–13.10; 12-<24 months: aRR = 2.00, 95% CI 1.35–2.96) increased risk of PBB. Baseline diagnoses of asthma/reactive airways disease (aRR = 0.30, 95% CI 0.26–0.35) or bronchiolitis (aRR = 0.15, 95% CI 0.06–0.38) decreased risk. M. catarrhalis was the most common organism (52.4%) identified in all children (PBB = 72.1%; no PBB = 50.2%, p < 0.001). We provide the first data on risks for PBB in children following acute illness and a hypothesis for studies to further investigate the relationship with wheeze-related illnesses. Clinicians and parents/guardians should be aware of these risks and seek early review if a wet cough lasting more than 4-weeks develops the post-acute illness.
Multidimensional Food Security Nexus in Drylands under the Slow Onset Effects of Climate Change
Authors: Stavi Ilan , Paschalidou Anastasia , Kyriazopoulos Apostolos P. , Halbac-Cotoara-Zamfir Rares , Siad Si Mokrane , Suska-Malawska Malgorzata , Savic Dragisa , Roque de Pinho Joana , Thalheimer Lisa , Williams David Samuel , Hashimshony-Yaffe Nurit , van der Geest Kees , Cordovil Claudia M. d. S. , Ficko Andrej
Published: December 2021

Hyperarid, arid, semiarid, and dry subhumid areas cover approximately 41% of the global land area. The human population in drylands, currently estimated at 2.7 billion, faces limited access to sufficient, affordable, and nutritious food. We discuss the interlinkages among water security, environmental security, energy security, economic security, health security, and food security governance, and how they affect food security in drylands. Reliable and adequate water supply, and the prevention of water contamination, increase the potential for ample food, fodder, and fiber production. Protecting woodlands and rangelands increases food security by buffering the slow onset effects of climate change, including biodiversity loss, desertification, salinization, and land degradation. The protection of natural lands is expected to decrease environmental contamination, and simultaneously, reduce the transfer of diseases from wildlife to humans. Biofuel production and hydroelectric power plants increase energy security but generate land-use conflicts, deforestation, and ecosystem degradation. Economic security generally positively correlates with food security. However, economic growth often degrades the environment, changes tenure rights over natural resources, and stimulates migration to urban areas, resulting in lower food and health security. Moreover, civil unrest, political instability, and armed conflicts disrupt local economies in drylands. Maintaining food security is crucial for health security; conversely, malnourished populations and unresponsive health systems decrease economic security, and adversely affect environmental, energy, and food security. Climate change is expected to deteriorate health security by spreading vector-borne diseases. Effective governance and timely interventions can substantially shorten periods of food insecurity, lower their intensities, and accelerate recovery from inevitable crises, and are therefore crucial in preventing humanitarian crises. Since global drylands population will nearly double by 2050, and since drylands are among the most susceptible areas to climate change, integrated multi-hazard approaches to food security are needed.
Conceptions of Assessment as an Integral Part of Language Learning: A Case Study of Finnish and Chinese University Students
Authors: Liontou Magdalini
Published: December 2021

Assessment is viewed as an internal and pivotal part of learning, where cultural factors, previous experiences, and future aspirations affect learners’ perceptions. In recent years, an increasing number of western universities have established their campuses or “dual” programmes in China. In the first Sino–Finnish programme, 293 Finnish and Chinese students participated in the same English for Specific Purposes (ESP) course. This study investigated students’ perceptions of assessment through an adapted version of the “Students’ Conceptions of Assessment” inventory, and it explored if the responses on each conception differ between the groups. The self-report inventory included statements based on four main conceptions of assessment: improvement, external factors, affect/benefit, and irrelevance, while open-ended questions were also included. The analysis of the open-ended questions raised the issues of teacher fairness, learner autonomy, and feedback. Additionally, differences appeared between the role of assessment and its relation to future aspirations, as well as the role of the parents. This study is a starting point for exploring the conceptions for distinct groups of students regarding assessment, providing a better understanding of students’ perceptions and discussing the implications for the language classroom.
Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells
Authors: Filardo Simone , Di Pietro Marisa , Frasca Federica , Diaco Fabiana , Scordio Mirko , Antonelli Guido , Scagnolari Carolina , Sessa Rosa
Published: December 2021

Following a Chlamydia trachomatis infection, the host immune response is characterized by its recognition via Toll-like and Nod-like Receptors, and the subsequent activation of interferon (IFN)-γ-mediated signaling pathways. Recently, the inflammasome-mediated host cell response has emerged to play a role in the physiopathology of C. trachomatis infection. Here we investigated, for the first time, the interaction of IFN-γ and inflammasome in an in vitro model of C. trachomatis-infected primary human synovial cells. Chlamydial replication as well as the expression of caspase-1, IL-1β, as well as IL-18 and IL-6, were assayed. Our results demonstrated the inhibitory activity of IFN-γ by interfering with the inflammasome network through the downregulation of caspase-1 mRNA expression. In addition, the ability of C. trachomatis to hinder the inflammasome pathway favoring its intracellular survival within synovial cells, was observed. Overall, our data suggest a potential mechanism of immune evasion by C. trachomatis in synovial cells, that may be contested by IFN-γ.
Creep and Shrinkage Behaviour of Disintegrated and Non-Disintegrated Cement Mortar
Authors: Gailitis Rihards , Figiela Beata , Abelkalns Kalvis , Sprince Andina , Sahmenko Genadijs , Choinska Marta , Guigou Martin Duarte
Published: December 2021

One way to prevent cement from ending up in landfills after its shelf life is to regain its activity and reuse it as a binder. As has been discovered, milling by planetary ball mill is not effective. Grinding by collision is considered a more efficient way to refine brittle material and, in the case of cement, to regain its activity. There has been considerable research regarding the partial replacement of cement using disintegrated cement in mortar or concrete in the past few decades. This article determines and compares the creep and shrinkage properties of cement mortar specimens made from old disintegrated, old non-disintegrated, and new non-disintegrated Portland cement. The tests show that the creep strains for old disintegrated and old non-disintegrated cement mortars are close, within a 2% margin of each other. However, the creep strains for new non-disintegrated cement mortar are 30% lower. Shrinkage for old disintegrated and non-disintegrated cement mortar is 20% lower than for new non-disintegrated cement mortar. The research shows that disintegration is a viable procedure to make old cement suitable for structural application from a long-term property standpoint. Additionally, it increases cement mortar compressive strength by 49% if the cement is disintegrated together with sand.
The Design of a Reflection Electron Energy Loss Spectrometer Attachment for Low Voltage Scanning Electron Microscopy
Authors: Chuah Jonathan , Khursheed Anjam
Published: December 2021

This paper presents the design of a reflection electron energy spectrometer (REELS) attachment for low voltage scanning electron microscopy (LVSEM) applications. The design is made by carrying out a scattered electron trajectory ray paths simulation. The spectrometer attachment is small enough to fit on the specimen stage of an SEM, and aims to acquire nanoscale spatially resolved REELS information. It uses a retarding field electrostatic toroidal sector energy analyzer design, which is able to lower the kinetic energies of elastically backscattered electrons to pass energies of 10 eV or less. For the capture of 1 keV BSEs emitted in the polar angular range between 40 to 50°, direct ray-tracing simulations predict that the spectrometer attachment will have an energy resolution of around 0.4 eV at a pass energy of 10 eV, and 0.2 eV at a pass energy of 5 eV. This predicted performance will make it a suitable REELS attachment for SEMs that use field emission electron sources.
Ultra-Short Laser Surface Properties Optimization of Biocompatibility Characteristics of 3D Poly-ε-Caprolactone and Hydroxyapatite Composite Scaffolds
Authors: Daskalova Albena , Filipov Emil , Angelova Liliya , Stefanov Radostin , Tatchev Dragomir , Avdeev Georgi , Sotelo Lamborghini , Christiansen Silke , Sarau George , Leuchs Gerd , Iordanova Ekaterina , Buchvarov Ivan
Published: December 2021

The use of laser processing for the creation of diverse morphological patterns onto the surface of polymer scaffolds represents a method for overcoming bacterial biofilm formation and inducing enhanced cellular dynamics. We have investigated the influence of ultra-short laser parameters on 3D-printed poly-ε-caprolactone (PCL) and poly-ε-caprolactone/hydroxyapatite (PCL/HA) scaffolds with the aim of creating submicron geometrical features to improve the matrix biocompatibility properties. Specifically, the present research was focused on monitoring the effect of the laser fluence (F) and the number of applied pulses (N) on the morphological, chemical and mechanical properties of the scaffolds. SEM analysis revealed that the femtosecond laser treatment of the scaffolds led to the formation of two distinct surface geometrical patterns, microchannels and single microprotrusions, without triggering collateral damage to the surrounding zones. We found that the microchannel structures favor the hydrophilicity properties. As demonstrated by the computer tomography results, surface roughness of the modified zones increases compared to the non-modified surface, without influencing the mechanical stability of the 3D matrices. The X-ray diffraction analysis confirmed that the laser structuring of the matrices did not lead to a change in the semi-crystalline phase of the PCL. The combinations of two types of geometrical designs—wood pile and snowflake—with laser-induced morphologies in the form of channels and columns are considered for optimizing the conditions for establishing an ideal scaffold, namely, precise dimensional form, mechanical stability, improved cytocompatibility and antibacterial behavior.
Implicit Finite Difference Simulation of Prandtl-Eyring Nanofluid over a Flat Plate with Variable Thermal Conductivity: A Tiwari and Das Model
Authors: Abu-Hamdeh Nidal H. , Aljinaidi Abdulmalik A. , Eltaher Mohamed A. , Almitani Khalid H. , Alnefaie Khaled A. , Abusorrah Abdullah M. , Safaei Mohammad Reza
Published: December 2021

The current article presents the entropy formation and heat transfer of the steady Prandtl-Eyring nanofluids (P-ENF). Heat transfer and flow of P-ENF are analyzed when nanofluid is passed to the hot and slippery surface. The study also investigates the effects of radiative heat flux, variable thermal conductivity, the material’s porosity, and the morphologies of nano-solid particles. Flow equations are defined utilizing partial differential equations (PDEs). Necessary transformations are employed to convert the formulae into ordinary differential equations. The implicit finite difference method (I-FDM) is used to find approximate solutions to ordinary differential equations. Two types of nano-solid particles, aluminium oxide (Al2O3) and copper (Cu), are examined using engine oil (EO) as working fluid. Graphical plots are used to depict the crucial outcomes regarding drag force, entropy measurement, temperature, Nusselt number, and flow. According to the study, there is a solid and aggressive increase in the heat transfer rate of P-ENF Cu-EO than Al2O3-EO. An increment in the size of nanoparticles resulted in enhancing the entropy of the model. The Prandtl-Eyring parameter and modified radiative flow show the same impact on the radiative field.
Experimental Assessment of Leptomeningeal Metastasis Diagnosis in Medulloblastoma Using Cerebrospinal Fluid Metabolomic Profiles
Authors: Im Ji Hye , Yoo Byong Chul , Lee Jun Hwa , Lee Kyue-Yim , Kim Kyung-Hee , Kim Jong Heon , Park Hyeon Jin , Park Meerim , Lee Sang Hyeon , Kwon Ji-Woong , Shin Sang Hoon , Yoo Heon , Yang Jeyul , Choi Seung Ah , Kim Seung-Ki , Gwak Ho-Shin
Published: December 2021

Diagnosing leptomeningeal metastasis (LM) in medulloblastoma is currently based on positive cerebrospinal fluid (CSF) cytology or magnetic resonance imaging (MRI) finding. However, the relevance of discordant results has not been established. We evaluated the diagnostic potential of CSF metabolomic profiles in the medulloblastoma LM assessment. A total of 83 CSF samples from medulloblastoma patients with documented MRI and CSF cytology results at the time of sampling for LM underwent low-mass ions (LMIs) analysis using liquid chromatography-mass spectrometry. Discriminating LMIs were selected by a summed sensitivity and specificity (>160%) and LMI discriminant equation (LOME) algorithms, evaluated by measuring diagnostic accuracy for verifying LM groups of different MRI/cytology results. Diagnostic accuracy of LM in medulloblastoma was 0.722 for cytology and 0.889 for MRI. Among 6572 LMIs identified in all sample, we identified 27 discriminative LMIs differentiating MRI (+)/cytology (+) from MRI (−)/cytology (−). Using LMI discriminant equation (LOME) analysis, we selected 9 LMIs with a sensitivity of 100% and a specificity of 93.6% for differentiating MRI (+)/cytology (+) from MRI (−)/cytology (−). Another LOME of 20 LMIs significantly differentiated sampling time relative to treatment (p = 0.007) and the presence or absence of LM-related symptoms (p = 0.03) in the MRI (+)/cytology (−) group. CSF metabolomics of medulloblastoma patients revealed significantly different profiles among LM diagnosed with different test results. We suggest that LM patients could be screened by appropriately selected LOME-generated LMIs to support LM diagnosis by either MRI or cytology alone.
Insight into OroxylinA-7-O-β-d-Glucuronide-Enriched Oroxylum indicum Bark Extract in Oral Cancer HSC-3 Cell Apoptotic Mechanism: Role of Mitochondrial Microenvironment
Authors: Kameyanda Poonacha Sharmila , Harishkumar Madhyastha , Radha Madhyastha , Varadarajan Remya , Nalilu Suchetha Kumari , Shetty Shilpa Sharathraj , Shetty Praveen Kumar , Chandrashekharappa Revanasiddappa Bistuvalli , Sreenivas Mahendra Gowdru , Bhandary Bavabeedu Satheesh Kumar
Published: December 2021

Oroxylum indicum, of the Bignoniaceae family, has various ethnomedical uses such as an astringent, anti-inflammatory, anti-bronchitis, anti-helminthic and anti-microbial, including anticancer properties. The druggability of OI stem bark extract was determined by its molecular docking interactions with PARP and Caspase-3, two proteins involved in cell survival and death. Note that 50 µg/mL of Oroxylum indicum extract (OIE) showed a significant (p < 0.05%) toxicity to HSC-3 cells. MTT aided cell viability and proliferation assay demonstrated that 50 µg/mL of OIE displayed significant (p < 0.5%) reduction in cell number at 4 h of incubation time. Cell elongation and spindle formation was noticed when HSC-3 cells were treated with 50 µg/mL of OIE. OIE initiated DNA breakage and apoptosis in HSC-3 cells, as evident from DNA ladder assay and calcein/EB staining. Apoptosis potential of OIE is confirmed by flow cytometer and triple-staining (live cell/apoptosis/necrosis) assay. Caspase-3/7 fluorescence quenching (LANCE) assay demonstrated that 50 µg/mL of OIE significantly enhanced the RFU of caspases-3/7, indicating that the apoptosis potential of OIE is probably through the activation of caspases. Immuno-cytochemistry of HSC-3 cells treated with 50 µg/mL of OIE showed a significant reduction in mitochondrial bodies as well as a reduction in RFU in 60 min of incubation time. Immunoblotting studies clearly showed that treatment of HSC-3 cells with OI extract caused caspase-3 activation and PARP deactivation, resulting in apoptotic cell death. Overall, our data indicate that OIE is an effective apoptotic agent for human squamous carcinoma cells and it could be a future cancer chemotherapeutic target.
Concurrent Measurement of Mitochondrial DNA Copy Number and ATP Concentration in Single Bovine Oocytes
Authors: Read Casey C. , Bhandari Sadikshya , Moorey Sarah E.
Published: December 2021

To sustain energy-demanding developmental processes, oocytes must accumulate adequate stores of metabolic substrates and mitochondrial numbers prior to the initiation of maturation. In the past, researchers have utilized pooled samples to study oocyte metabolism, and studies that related multiple metabolic outcomes in single oocytes, such as ATP concentration and mitochondrial DNA copy number, were not possible. Such scenarios decreased sensitivity to intraoocyte metabolic relationships and made it difficult to obtain adequate sample numbers during studies with limited oocyte availability. Therefore, we developed and validated procedures to measure both mitochondrial DNA (mtDNA) copy number and ATP quantity in single oocytes. Validation of our procedures revealed that we could successfully divide oocyte lysates into quarters and measure consistent results from each of the aliquots for both ATP and mtDNA copy number. Coefficient of variation between the values retrieved for mtDNA copy number and ATP quantity quadruplicates were 4.72 ± 0.98 and 1.61 ± 1.19, respectively. We then utilized our methodology to concurrently measure mtDNA copy number and ATP quantity in germinal vesicle (GV) and metaphase two (MII) stage oocytes. Our methods revealed a significant increase in ATP levels (GV = 628.02 ± 199.53 pg, MII = 1326.24 ± 199.86 pg, p < 0.001) and mtDNA copy number (GV = 490,799.4 ± 544,745.9 copies, MII = 1,087,126.9 ± 902,202.8 copies, p = 0.035) in MII compared to GV stage oocytes. This finding is consistent with published literature and provides further validation of the accuracy of our methods. The ability to produce consistent readings and expected results from aliquots of the lysate from a single oocyte reveals the sensitivity and feasibility of using this method.
A Content Review of Life Cycle Assessment of Nanomaterials: Current Practices, Challenges, and Future Prospects
Authors: Nizam Nurul Umairah M. , Hanafiah Marlia M. , Woon Kok Sin
Published: December 2021

This paper provides a comprehensive review of 71 previous studies on the life cycle assessment (LCA) of nanomaterials (NMs) from 2001 to 2020 (19 years). Although various studies have been carried out to assess the efficiency and potential of wastes for nanotechnology, little attention has been paid to conducting a comprehensive analysis related to the environmental performance and hotspot of NMs, based on LCA methodology. Therefore, this paper highlights and discusses LCA methodology’s basis (goal and scope definition, system boundary, life cycle inventory, life cycle impact assessment, and interpretation) to insights into current practices, limitations, progress, and challenges of LCA application NMs. We found that there is still a lack of comprehensive LCA study on the environmental impacts of NMs until end-of-life stages, thereby potentially supporting misleading conclusions, in most of the previous studies reviewed. For a comprehensive evaluation of LCA of NMs, we recommend that future studies should: (1) report more detailed and transparent LCI data within NMs LCA studies; (2) consider the environmental impacts and potential risks of NMs within their whole life cycle; (3) adopt a transparent and prudent characterization model; and (4) include toxicity, uncertainty, and sensitivity assessments to analyze the exposure pathways of NMs further. Future recommendations towards improvement and harmonization of methodological for future research directions were discussed and provided. This study’s findings redound to future research in the field of LCA NMs specifically, considering that the release of NMs into the environment is yet to be explored due to limited understanding of the mechanisms and pathways involved.
Nanolasers with Feedback as Low-Coherence Illumination Sources for Speckle-Free Imaging: A Numerical Analysis of the Superthermal Emission Regime
Authors: Wang Tao , Jiang Can , Zou Junlong , Yang Jie , Xu Kuiwen , Jin Chaoyuan , Wang Gaofeng , Puccioni Gian Piero , Lippi Gian Luca
Published: December 2021

Lasers distinguish themselves for the high coherence and high brightness of their radiation, features which have been exploited both in fundamental research and a broad range of technologies. However, emerging applications in the field of imaging, which can benefit from brightness, directionality and efficiency, are impaired by the speckle noise superimposed onto the picture by the interference of coherent scattered fields. We contribute a novel approach to the longstanding efforts in speckle noise reduction by exploiting a new emission regime typical of nanolasers, where low-coherence laser pulses are spontaneously emitted below the laser threshold. Exploring the dynamic properties of this kind of emission in the presence of optical reinjection we show, through the numerical analysis of a fully stochastic approach, that it is possible to tailor some of the properties of the emitted radiation, in addition to exploiting this naturally existing regime. This investigation, therefore, proposes semiconductor nanolasers as potential attractive, miniaturized and versatile future sources of low-coherence radiation for imaging.
Calculating the Effect of AlGaN Dielectric Layers in a Polarization Tunnel Junction on the Performance of AlGaN-Based Deep-Ultraviolet Light-Emitting Diodes
Authors: Wang Yong , Zhang Zihui , Guo Long , Chen Yuxuan , Li Yahui , Qi Zhanbin , Ben Jianwei , Sun Xiaojuan , Li Dabing
Published: December 2021

In this work, AlGaN-based deep-ultraviolet (DUV) light-emitting diodes (LEDs) with AlGaN as the dielectric layers in p+-Al0.55Ga0.45N/AlGaN/n+-Al0.55Ga0.45N polarization tunnel junctions (PTJs) were modeled to promote carrier tunneling, suppress current crowding, avoid optical absorption, and further enhance the performance of LEDs. AlGaN with different Al contents in PTJs were optimized by APSYS software to investigate the effect of a polarization-induced electric field (Ep) on hole tunneling in the PTJ. The results indicated that Al0.7Ga0.3N as a dielectric layer can realize a higher hole concentration and a higher radiative recombination rate in Multiple Quantum Wells (MQWs) than Al0.4Ga0.6N as the dielectric layer. In addition, Al0.7Ga0.3N as the dielectric layer has relatively high resistance, which can increase lateral current spreading and enhance the uniformity of the top emitting light of LEDs. However, the relatively high resistance of Al0.7Ga0.3N as the dielectric layer resulted in an increase in the forward voltage, so much higher biased voltage was required to enhance the hole tunneling efficiency of PTJ. Through the adoption of PTJs with Al0.7Ga0.3N as the dielectric layers, enhanced internal quantum efficiency (IQE) and optical output power will be possible.
Low Malaria Transmission in Rosso, an Irrigated Rice-Growing Area in Mauritania
Authors: Ould Lemrabott Mohamed Aly , Mint Lekweiry Khadijetou , Deida Jemila , Djigo Oum Kelthoum Mamadou , Ould Ahmedou Salem Mohamed Salem , Ould Khalef Yacoub , Bogreau Hervé , Simard Frédéric , Basco Leonardo , Ould Mohamed Salem Boukhary Ali
Published: December 2021

The construction of dams along the Senegal River resulted in an increase in irrigated land areas and changes in the epidemiology and transmission of water-related diseases. The objective of this study was to update epidemiological data on malaria in Rosso, one of the major Mauritanian cities along the Senegal River. Febrile patients (n = 318) were screened for malaria using a rapid diagnostic test (RDT) for malaria and microscopic examination of blood smears. Diagnosis was later confirmed by polymerase chain reaction (PCR). The mean age of 318 febrile patients was 25.1 (range 1–80 years). Only 7 (2.2%) and 2 (0.6%) had a positive RDT and thick smear, respectively. PCR confirmed the diagnosis in two Plasmodium vivax-infected patients. Most participants (198/318, 62.3%) had no recent travel history outside Rosso. The majority of the febrile patients (90%, 284/311) owned at least one insecticide-treated net (ITN). The frequency of the use of ITNs was not significantly associated with season (rainy vs. dry seasons; p = 0.9) or with the number of ITNs per household (rs = 0.07; n = 285; p = 0.19). Of 285 individuals with ITNs, only two (0.7%) with no travel history were PCR-positive for malaria. Despite the presence of mosquito breeding sites related to rice irrigation, malaria transmission in Rosso remained very low, possibly due to the high coverage and frequent use of bed nets. Regular entomological surveillance for possible changes in the prevalence of Anopheles mosquito species and their behavioural aspects should be implemented.
Conceptualization of a Sensory Feedback System in an Anthropomorphic Replacement Hand
Authors: Hazubski Simon , Bamerni Derya , Otte Andreas
Published: December 2021

(1) Background: This paper presents a conceptual design for an anthropomorphic replacement hand made of silicone that integrates a sensory feedback system. In combination with a motorized orthosis, it allows performing movements and registering information on the flexion and the pressure of the fingers. (2) Methods: To create the replacement hand, a three-dimensional (3D) scanner was used to scan the hand of the test person. With computer-aided design (CAD), a mold was created from the hand, then 3D-printed. Bending and force sensors were attached to the mold before silicone casting to implement the sensory feedback system. To achieve a functional and anthropomorphic appearance of the replacement hand, a material analysis was carried out. In two different test series, the properties of the used silicones were analyzed regarding their mechanical properties and the manufacturing process. (3) Results: Individual fingers and an entire hand with integrated sensors were realized, which demonstrated in several tests that sensory feedback in such an anthropomorphic replacement hand can be realized. Nevertheless, the choice of silicone material remains an open challenge, as there is a trade-off between the hardness of the material and the maximum mechanical force of the orthosis. (4) Conclusion: Apart from manufacturing-related issues, it is possible to cost-effectively create a personalized, anthropomorphic replacement hand, including sensory feedback, by using 3D scanning and 3D printing techniques.
Sorption Processes in Soils and Sediments
Authors: Elzinga Evert J. , Arai Yuji
Published: December 2021

Sorption processes at the mineral–water interface are fundamental to the chemical functioning of soils, and impact the biogeochemical cycling of both trace and major elements in soil and sediment environments [...]
Approaches and Policies to Promote Zero-Waste City Construction: China’s Practices and Lessons
Authors: Meng Mingfu , Wen Zongguo , Luo Weijun , Wang Shijie
Published: December 2021

The rapidly growing output of solid waste has brought tremendous pressure to urban development. China launched an action plan known as “Zero-waste city” (ZWC), that refers to an urban development model aimed at reducing the generation of and enhancing the recycling of solid waste, in order to alleviate environmental impacts. Eleven cities and five special zones achieved positive results of solid waste management were selected as pilot areas for exploring empirical methods until 2019. The practices and lessons of the pilot cities need to be deeply analyzed and summarized, so as to promote successful models, learn lessons and better implement the policy comprehensively for other cities. This study presents a review of China’s ZWC policies and practices with constructive suggestions for further development. Based on the policy objective of ZWC and the field investigation of solid waste flow, five crucial approaches to developing ZWC are proposed, namely, solid waste reduction throughout the industrial chain, collaborative treatment of classified municipal solid waste (MSW), efficient utilization of agricultural waste with multiple purposes, safety control of hazardous waste flow, and optimization of market mechanisms. The case study demonstrates that the five paths are appropriate to Xuzhou city. However, deficiencies in MSW classification, pesticide packaging waste collection systems, solid waste product application, management, and policymaking, have emerged. Regulatory prohibitions, extended producer responsibility and market vitality should be adopted to improve the collection, transportation, and utilization of solid waste. Key findings from this research are to summarize crucial paths toward fulfiling ZWC goal, and to reveal some successful practices of, and lessons from ZWC construction by case studies. This study provides a method to further implement zero solid waste management in a targeted manner. The recommendations drawn from the study, which include law, market and institutional measures, may contribute to the achievement of developing sustainable cities.
Risk Assessment Models to Improve Environmental Safety in the Field of the Economy and Organization of Construction: A Case Study of Russia
Authors: Larionov Arkadiy , Nezhnikova Ekaterina , Smirnova Elena
Published: December 2021

This article assesses risks in order to substantiate the economic and organizational efficiency of housing and industrial construction. This topic is relevant because it is necessary for sustainable development. In Russia, environmental safety in construction and housing, as well as communal services, is poorly developed and not regulated by the legal system. As building construction, housing, and communal services should be based on environmental safety, this topic requires rapid development. Methods related to quantifying environmental risk and making decisions under conditions of uncertainty were studied. A quantitative risk assessment was performed using the Monte Carlo method for pessimistic and optimistic options to prevent environmental damage. The model reproduced the distribution derived from the evidence-based fit. The results of sensitivity analysis are also presented to prove the hypothesis. The selection of the most appropriate probability density functions for each of the input quantities was implemented through settings in a computer program. The simulation modeling results clearly illustrate the choice of the general principle of assessment and the adoption of the optimal decision. In conditions of uncertainty, the decision to choose the optimistic options with high cost (to maintain the reliability of the technical system) but less risk plays a decisive role in the future environmental safety strategies of construction projects. The Monte Carlo method is preferable for environmental impact assessments. In the future, the amended methodology can be applied to raise environmental safety in the field of construction.
Facilitating Servitization in Manufacturing Firms: The Influence of Strategic Orientation
Authors: Zhang Yu , Wang Yajuan , Li Yao
Published: December 2021

Servitization has significant implications for the sustainable development of manufacturing, the economy, and the environment. However, it does not always produce returns as the firms expect, which may discourage them from engaging in this transition. In this study, we examine the facilitating effects of two dimensions of strategic orientation (i.e., technology and market orientation) on two types of servitization (i.e., basic and advanced service provision), and further investigate the performance impacts of these servitization types contingent on firm size. By conducting an empirical study, using survey data comprising 210 samples, we confirm that both technology and market orientation are positively related to basic and advanced service provision. Moreover, while they have equal effects on basic services, market orientation is more important than technology orientation for providing advanced services. We also find that, for basic services, these two strategic orientations function independently, whereas they reinforce each other in the provision of advanced services. Finally, the relationship between servitization and firm performance is contingent on the size of the firm. Our results show that small firms can benefit from providing basic services, rather than advanced services, while only advanced services can improve the performance of large firms further.