bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of treatment persistence, adherence, and risk of exacerbation in patients with COPD treated with single-inhaler versus multiple-inhaler triple therapy: A prospective observational study in China
Authors: Lin Ling , Liu Cong , Cheng Wei , Song Qing , Zeng Yuqin , Li Xin , Deng Dingding , Liu Dan , Chen Yan , Cai Shan , Chen Ping
Published: March 2023

Aim: This study sought to compare treatment persistence, adherence, and risk of exacerbation among patients with COPD treated with single-inhaler triple therapy (SITT) and multiple-inhaler triple therapy (MITT) in the Chinese population.

Methods: This was a multicenter, prospective observational study. Patients with COPD from ten hospitals in Hunan and Guangxi provinces in China were recruited from 1 January 2020 to 31 November 2021 for the study and were followed up for one year. Treatment persistence, adherence, and exacerbation rates during the 12-month follow-up were analyzed in COPD patients treated with SITT and MITT.

Results: A total of 1,328 patients were enrolled for final analysis, including 535 (40.3%) patients treated with SITT and 793 (59.7%) treated with MITT. Of these patients, the mean age was 64.9 years and most patients were men. The mean CAT score was 15.2 ± 7.1, and the median (IQR) FEV1% was 54.4 (31.2). The SITT group had a higher mean CAT score, more patients with mMRC >1, and lower mean FEV1% and FEV1/FVC than the MITT patients. Moreover, the proportion of patients with ≥1 exacerbation in the previous year was higher in the SITT cohort. SITT patients had, compared to MITT patients, a higher proportion of adherence (proportion of days covered, PDC) ≥0.8 (86.5% vs. 79.8%; p = 0.006), higher treatment persistence [HR: 1.676 (1.356–2.071), p < 0.001], lower risk of moderate-to-severe exacerbation [HR: 0.729 (0.593–0.898), p = 0.003], and severe exacerbation [HR: 0.675 (0.515–0.875), p = 0.003], as well as reduced all-cause mortality risk [HR: 0.475 (0.237-0.952), p = 0.036] during the 12-month follow-up. Persistence was related to fewer future exacerbations and mortality than non-persistence in the SITT and MITT groups.

Conclusion: Patients with COPD treated with SITT showed improved treatment persistence and adherence, as well as a reduction in the risk of moderate-to-severe exacerbation, severe exacerbation, and mortality compared to patients treated with MITT in the Chinese population.

Clinical Trial Registration: https://www.chictr.org.cn/, identifier ChiCTR-POC-17010431.
Curative effect of anti-fibrosis Chinese patent medicines combined with ursodeoxycholic acid for primary biliary cholangitis: A systematic review and meta-analysis
Authors: Bi Yufei , Shi Ke , Chen Jialiang , Wang Xianbo
Published: March 2023

Objective: To delineate the curative effect and safety of anti-fibrosis Chinese patent medicines (CPMs) combined with ursodeoxycholic acid (UDCA) for primary biliary cholangitis (PBC).

Methods: A literature search was conducted using PubMed, Web of Science, Embase, Cochrane Library, Wanfang database, VIP database, China Biology Medicine Database, and Chinese National Knowledge Infrastructure from their inception until August 2022. Randomized controlled trials (RCTs) of the treatment of PBC with anti-fibrotic CPMs were collected. The eligibility of the publications was assessed using the Cochrane risk-of-bias tool. The evaluation indicators were the clinical efficacy rate, liver fibrosis, liver function, immune function, and symptom score. Meta-analysis and subgroup analysis were conducted to evaluate the effectiveness of anti-fibrosis CPMs. Risk ratio (RR) was used to assess dichotomous variables, and continuous variables with a 95% confidence interval were calculated using mean difference.

Results: Twenty-two RCTs including 1,725 patients were selected. The findings demonstrated that anti-fibrotic CPMs combined with UDCA improved the efficacy rate, liver function, liver fibrosis, immunological indicators, and clinical symptoms compared with UDCA alone (all p < 0.05).

Conclusion: This study demonstrates that the combination of anti-fibrotic CPMs and UDCA can improve both clinical symptoms and outcomes. Nevertheless, more high-quality RCTs are needed to assess the effectiveness of anti-fibrosis CPMs for PBC.
New insights into the phylogenetic relationships among wild onions (Allium, Amaryllidaceae), with special emphasis on the subgenera Anguinum and Rhizirideum, as revealed by plastomes
Authors: Yang JiYoung , Kim Seon-Hee , Gil Hee-Young , Choi Hyeok-Jae , Kim Seung-Chul
Published: March 2023

The genus Allium, with over 900 species, is one of the largest monocotyledonous genera and is widely accepted with 15 recognized subgenera and 72 sections. The robust subgeneric and sectional relationships within Allium have long been not resolved. Based on 76 species of Allium (a total of 84 accessions), we developed a highly resolved plastome phylogenetic framework by integrating 18 newly sequenced species (20 accessions) in this study and assessed their subgeneric and sectional relationships, with special emphasis on the two subgenera Anguinum and Rhizirideum. We retrieved the three major evolutionary lines within Allium and found that the two subgenera Anguinum and Rhizirideum are monophyletic whereas others are highly polyphyletic (e.g., Allium, Cepa, Polyprason, and Melanocrommyum). Within the subgenus Anguinum, two strongly supported sublineages in East Asian and Eurasian-American were found. Allium tricoccum in North America belonged to the Eurasian clade. The distinct taxonomic status of A. ulleungense and its sister taxon were further determined. In subg. Rhizirideum, the Ulleung Island endemic A. dumebuchum shared its most recent common ancestor with the species from Mongolia and the narrow Korean endemic A. minus. Two Ulleung Island endemics were estimated to originate independently during the Pleistocene. In addition, a separate monotypic sectional treatment of the east Asian A. macrostemon (subg. Allium) and sister relationship between A. condensatum and A. chinense was suggested.
Variation in gene expression along an elevation gradient of Rhododendron sanguineum var. haemaleum assessed in a comparative transcriptomic analysis
Authors: Ye Lin-Jiang , Möller Michael , Luo Ya-Huang , Zou Jia-Yun , Zheng Wei , Liu Jie , Li De-Zhu , Gao Lian-Ming
Published: March 2023

Selection along environmental gradients may play a vital role in driving adaptive evolution. Nevertheless, genomic variation and genetic adaptation along environmental clines remains largely unknown in plants in alpine ecosystems. To close this knowledge gap, we assayed transcriptomic profiles of late flower bud and early leaf bud of Rhododendron sanguineum var. haemaleum from four different elevational belts between 3,000 m and 3,800 m in the Gaoligong Mountains. By comparing differences in gene expression of these samples, a gene co-expression network (WGCNA) was constructed to identify candidate genes related to elevation. We found that the overall gene expression patterns are organ-specific for the flower and leaf. Differentially expressed unigenes were identified in these organs. In flowers, these were mainly related to terpenoid metabolism (RsHMGR, RsTPS), while in leaves mainly related to anthocyanin biosynthesis (RsCHS, RsF3’5’H). Terpenoids are the main components of flower scent (fragrance) likely attracting insects for pollination. In response to fewer pollinators at higher elevation zone, it seems relatively less scent is produced in flower organs to reduce energy consumption. Secondary metabolites in leaves such as anthocyanins determine the plants’ alternative adaptive strategy to extreme environments, such as selective pressures of insect herbivory from environmental changes and substrate competition in biosynthesis pathways at high elevations. Our findings indicated that the gene expression profiles generated from flower and leaf organs showed parallel expression shifts but with different functionality, suggesting the existence of flexibility in response strategies of plants exposed to heterogeneous environments across elevational gradients. The genes identified here are likely to be involved in the adaptation of the plants to these varying mountainous environments. This study thus contributes to our understanding of the molecular mechanisms of adaptation in response to environmental change.
Multi-instrumental analysis of the day-to-day variability of equatorial plasma bubbles
Authors: Aa Ercha , Zhang Shun-Rong , Coster Anthea J. , Erickson Philip J. , Rideout William
Published: March 2023

This paper presents a multi-instrument observational analysis of the equatorial plasma bubbles (EPBs) variation over the American sector during a geomagnetically quiet time period of 07–10 December 2019. The day-to-day variability of EPBs and their underlying drivers are investigated through coordinately utilizing the Global-scale Observations of Limb and Disk (GOLD) ultraviolet images, the Ionospheric Connection Explorer (ICON) in-situ and remote sensing data, the global navigation satellite system (GNSS) total electron content (TEC) observations, as well as ionosonde measurements. The main results are as follows: 1) The postsunset EPBs’ intensity exhibited a large day-to-day variation in the same UT intervals, which was fairly noticeable in the evening of December 07, yet considerably suppressed on December 08 and 09, and then dramatically revived and enhanced on December 10. 2) The postsunset linear Rayleigh-Taylor instability growth rate exhibited a different variation pattern. It had a relatively modest peak value on December 07 and 08, yet a larger peak value on December 09 and 10. There was a 2-h time lag of the growth rate peak time in the evening of December 09 from other nights. This analysis did not show an exact one-to-one relationship between the peak growth rate and the observed EPBs intensity. 3) The EPBs’ day-to-day variation has a better agreement with that of traveling ionospheric disturbances and atmospheric gravity waves signatures, which exhibited relatively strong wavelike perturbations preceding/accompanying the observed EPBs on December 07 and 10 yet relatively weak fluctuations on December 08 and 09. These coordinate observations indicate that the initial wavelike seeding perturbations associated with AGWs, together with the catalyzing factor of the instability growth rate, collectively played important roles to modulate the day-to-day variation of EPBs. A strong seeding perturbation could effectively compensate for a moderate strength of Rayleigh-Taylor instability growth rate and therefore their combined effect could facilitate EPB development. Lacking proper seeding perturbations would make it a more inefficient process for the development of EPBs, especially with a delayed peak value of Rayleigh-Taylor instability growth rate.
Effect of preoperative radiotherapy on the prognosis of patients with stage cTxN0M0 esophageal squamous cell carcinoma: propensity score matching analysis based on SEER database
Authors: Jin Zixian , Sun Jiajing , Zhang Jian , Shen Jianfei , Zhang Bo
Published: March 2023

Objective

The aim of this study was to investigate the effect of preoperative radiotherapy (RT) on overall survival (OS) in patients with stage cTxN0M0 esophageal squamous cell carcinoma (ESCC).

Methods

A total of 467 patients with ESCC diagnosed as cTxN0M0 and undergoing esophagectomy between 2004 and 2016 were downloaded from the Surveillance, Epidemiology, and End Results (SEER) database. According to the presence or absence of preoperative RT, the patients were divided into preoperative RT group and non-preoperative RT group. Propensity score matching (PSM) was performed to equalize baseline levels between groups. Univariate and multivariate Cox regression analyses were used to compare the survival differences between the two groups.

Results

Using PSM, 162 pairs of patients were selected. Preoperative RT was not a prognostic factor for OS in all patients with cTx stage. After PSM, for patients with cT1–2 stage, univariate Cox regression analysis showed that preoperative RT was an influencing factor of OS, and multivariate Cox regression analysis confirmed that preoperative RT was an independent predictor of OS. Compared with non-preoperative RT, preoperative RT significantly decreased OS (HR = 1.556, 95%CI 1.008–2.464, p = 0.046). For patients with cT3–4, univariate Cox regression analysis showed that preoperative RT was an influencing factor for OS, and multivariate Cox regression analysis determined that preoperative RT was independent predictors of survival. Compared with non-preoperative RT, preoperative RT significantly improved the OS (HR = 0.479, 95%CI 0.272–0.841, p = 0.010).

Conclusion

For ESCC, preoperative RT can improve the OS of patients with cT3-4N0M0. However, preoperative RT is not suitable for patients with cT1-2N0M0.
A rare gas-containing lumbar synovial cyst treated by percutaneous transforaminal endoscopic cystectomy: A case report and literature review
Authors: Wei Ziran , Lin Qian , Zhang Hao , Zhang Haoyun , Ma Xuexiao , Wang Chao
Published: March 2023

Spinal synovial cysts are rare entities for which standard surgical strategies are inconsistent. Here, we present an uncommon intraspinal gas-containing synovial cyst treated by percutaneous transforaminal endoscopic cystectomy. A 52-year-old man presented with radicular pain and intermittent claudication that had persisted for one month. Computed tomography revealed an intraspinal cystic lesion anteromedial to the left L4-L5 articular joint and the center of the lesion manifested gas contents. A transforaminal endoscopic procedure was performed and confirmed as a safe and minimally invasive technique for gas-containing lumbar synovial cysts. It provides a valuable substitution and supplementation to open surgery.
Evaluating outcomes of patient-centered enhanced recovery after surgery (ERAS) in percutaneous nephrolithotomy for staghorn stones: An initial experience
Authors: Lei Jun , Huang Kai , Dai Yingbo , Yin Guangming
Published: March 2023

Objective

To evaluate the outcomes of patient-centered enhanced recovery after surgery (ERAS) in ­percutaneous nephrolithotomy (PCNL) for staghorn stones.

Patients and methods

A retrospective analysis of 106 patients with staghorn calculi who underwent PCNL treatment at the Third Xiangya Hospital from October 01, 2018 to September 30, 2021 was performed. The patients were divided into the ERAS group (n = 56) and traditional group (n = 50). The ERAS program focused on a patient-centered concept, with elaboration on aspects, such as patient education, nutritional support, analgesia, body warming, early mobilization, nephrostomy tube removal, and strict follow-up.

Results

The total stone free rate and total complication rate were similar in both groups. The visual analogue scale (VAS) 6 h after surgery, ambulation off bed time, indwelling ﬁstula time, indwelling catheter time, and postoperative hospital stays were lower in the ERAS group than in the traditional group (P < 0.05). The multiple session rate in the ERAS group (19, 28.57%) was lower than that in the traditional group (30, 60%) (P = 0.007). The 1-year stone recurrence rate in the ERAS group (7, 17.5%) was lower than that in the traditional group (14, 38.9%) (P = 0.037).

Conclusion

The patient-centered ERAS in PCNL for staghorn stones accelerated rehabilitation by relieving postoperative pain, shortening hospitalization time, accelerating early ambulation, and reducing multiple session rate and 1-year stone recurrence rate, which have socioeconomic benefits.
Developmental venous anomaly associated with dural arteriovenous fistula: Etiopathogenesis and hemorrhagic risk
Authors: Agosti Edoardo , De Maria Lucio , Panciani Pier Paolo , Serioli Simona , Mardighian Dikran , Fontanella Marco Maria , Lanzino Giuseppe
Published: March 2023

Introduction

Developmental venous anomalies (DVAs) have traditionally been defined as non-pathological congenital lesions. Compared to isolated DVAs, the association of DVAs with arteriovenous shunts seems to have a more adverse clinical connotation. In this review, we describe the association between DVA and dAVF and discuss the hemorrhagic risk. We also advance a hypothesis about the potential de novo formation of a DVA and challenge the dogma about their “developmental” or “congenital” nature.

Methods

A systematic review of the literature on the association of DVA and dAVF was performed in accordance with the PRISMA-P (Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols) guidelines.

Results

A number of 678 papers was initially identified, but only 9 studies were included in the final qualitative analysis. Most of the patients presented with bleeding (56%), with a median GCS of 14 (range 10–15). In 56% of the cases the DVA had a supratentorial location. Supratentorial DVAs mostly drained in the superior sagittal sinus (80%), while all of infratentorial/combined DVAs drained in deep ependymal veins of the 4th ventricle. All the supratentorial dAVFs drained into the superior sagittal sinus, while the infratentorial/combined dAVFs mostly drained in the jugular bulb, Vein of Rosenthal, or transverse-sigmoid sinuses (75%). Most of the dAVFs were classified as Cognard type IIa + b (67%), while in a smaller number of cases type I (22%) and type V (11%). The dAVF was the target of treatment in each case and most patients underwent endovascular treatment (78%). The dAVF was completely occluded in 78% of cases and no periprocedural complications were reported.

Conclusion

The clinical presentation, radiological findings, and treatment outcomes of DVAs and associated dAVFs have been discussed. Despite the general opinion that DVAs are benign congenital lesions, increasing epidemiological and radiological evidence supports a potential acquired origin, and the venous system seem to play a pivotal role in their post-natal genesis and development.
Grading pharmacists’ risk of complaints to a regulator: A retrospective cohort study
Authors: Morris Katherine , Spittal Matthew J.
Published: March 2023

Background: Tools to grade risk of complaint to a regulatory board have been developed for physicians but not for other health practitioner groups, including pharmacists. We aimed to develop a score that classified pharmacists into low, medium and high risk categories.

Methods: Registration and complaint data were sourced from Ontario College of Pharmacists for January 2009 to December 2019. We undertook recurrent event survival analysis to predict lodgement of a complaint. We identified those variables that were associated with a complaint and included these in a risk score which we called PRONE-Pharm (Predicted Risk of New Event for Pharmacists). We assessed diagnostic accuracy and used this to identify thresholds that defined low, medium and high risk.

Results: We identified 3,675 complaints against 17,308 pharmacists. Being male (HR = 1.72), older age (HR range 1.43–1.54), trained internationally (HR = 1.62), ≥1 prior complaint (HR range 2.83–9.60), and complaints about mental health or substance use (HR = 1.91), compliance with conditions (HR = 1.86), fees and servicing (HR = 1.74), interpersonal behaviour or honesty (HR = 1.40), procedures (HR = 1.75) and treatment or communication or other clinical issues (HR = 1.22) were all associated with lodgement of a complaint. When converted into the PRONE-Pharm risk score, pharmacists were assigned between 0 and 98 points with higher scores closely associated with higher probability of a complaint. A score of ≥25 had sufficient accuracy for classifying medium-risk pharmacists (specificity = 87.0%) and ≥45 for high-risk pharmacists (specificity = 98.4%).

Conclusion: Distinguishing isolated incidents from persistent problems poses a significant challenge for entities responsible for the regulation of pharmacists and other health practitioners. The diagnostic properties of PRONE-Pharm (minimizing the false positives) means that the risk score is useful for “ruling-out” low risk pharmacists using routinely collected regulatory data. PRONE-Pharm may be useful when used alongside interventions appropriately matched to a pharmacist’s level of risk.
How to Efficiently Produce the Selenium-Enriched Cucumber Fruit with High Yield and Qualities via Hydroponic Cultivation? The Balance between Selenium Supply and CO2 Fertilization
Authors: Wang Ziying , Li Di , Gruda Nazim S. , Zhu Chunwu , Duan Zengqiang , Li Xun
Published: March 2023

Hydroponic-producing selenium (Se)-biofortified vegetables in a greenhouse is a convenient and effective way to provide Se-enriched food and overcome hidden hunger. CO2 fertilization is commonly implemented to increase vegetable yield in greenhouse production. However, this application accompanies decreased mineral concentrations in the edible parts. Here we investigated the effects of [CO2] and Se supply on the growth, gas exchange, and cucumber fruit quality. A hydroponic experiment with two CO2 concentrations ([CO2]) (C1: 410, and C2: 1200 μmol mol−1) and four Se supply levels (Se0: 0, Se1: 0.125, Se2: 0.250, and Se3: 0.500 mg Se L−1) was carried out. A low level of Se supply (Se1: 0.125 mg Se L−1) protected the photosynthetic pigments and stimulated the stomatal opening, especially under [CO2] fertilization. It leads to a higher net photosynthesis rate (Pn) and transpiration rate (Tr) than other Se treatments. The most significant changes in dry weight, fruit yield, and soluble sugar concentration were also obtained in Se1 under CO2 fertilization due to the enhanced CO2 fixation. Meanwhile, the Se concentration in fruit was 0.63 mg kg−1 FW in C2Se1, with the highest Se accumulation and use efficiency. According to the recommended dietary allowance of 55 μg Se day−1 for adults, an intake of 87 g of cucumber grown in C2Se1 is sufficient. Because of the improved Tr and better root structure in Se1, the uptake of mineral nutrients through mass flow and interception was well maintained under CO2 fertilization. So, the concentrations of N, P, K, Ca, and Mn in cucumber fruits were not significantly decreased by elevated [CO2] in Se1. However, the concentrations of soluble proteins, S, Mg, Fe, and Zn in cucumber fruits in C2Se1 were lower than those in C1Se1, which was mainly attributed to the dilution effects under CO2 fertilization. Therefore, a selenite supply of 0.125 mg Se L−1 was found to be the optimal dosage for producing Se-enriched cucumber fruits with high yield and better qualities under CO2 fertilization (1200 μmol mol−1).
Understanding the Pathogenesis of Red Mark Syndrome in Rainbow Trout (Oncorhynchus mykiss) through an Integrated Morphological and Molecular Approach
Authors: Galeotti Marco , Orioles Massimo , Saccà Elena , Byadgi Omkar , Pesaro Stefano , Di Cerbo Alessandro , Magi Gian Enrico
Published: March 2023

Red mark syndrome (RMS) is a widespread skin disorder of rainbow trout in freshwater aquaculture, believed to be caused by a Midichloria-like organism (MLO). Here, we aimed to study the pathologic mechanisms at the origin of RMS by analyzing field samples from a recent outbreak through gene expression, MLO PCR, quantitative PCR, and a histopathological scoring system proposed for RMS lesions. Statistical analyses included a One-Way Analysis of Variance (ANOVA) with a Dunnett’s multiple comparisons test to assess differences among gene expression groups and a nonparametric Spearman correlation between various categories of skin lesions and PCR results. In short, the results confirmed the presence of a high quantity of 16S gene copy numbers of Midichloria-like organisms in diseased skin tissues. However, the number of Midichloria-like organisms detected was not correlated to the degree of severity of skin disease. Midichloria-like organism DNA was found in the spleen and head kidney. The spleen showed pathologic changes mainly of hyperplastic type, reflecting its direct involvement during infection. The most severe skin lesions were characterized by a high level of inflammatory cytokines sustaining and modulating the severe inflammatory process. IL-1 β, IL-6, IL-10, MHC-II, and TCR were upregulated in severe skin lesions, while IL-10 was highly expressed in moderate to severe ones. In the moderate form, the response was driven to produce immunoglobulins, which appeared crucial in controlling the skin disease’s severity. Altogether our results illustrated a complex immune interaction between the host and Midichloria-like organism.
Moringa oleifera Leaf Powder as New Source of Protein-Based Feedstuff Improves Growth Performance and Cecal Microbial Diversity of Broiler Chicken
Authors: Zhang Haiwen , Huang Liangmin , Hu Shihui , Qin Xinyun , Wang Xuemei
Published: March 2023

Currently, the lack of protein source feed has become a pressing issue. Moringa oleifera leaf powder (MOLP) has good potential for the development of protein-derived feeds due to its good protein quality and abundance, but little is known about its effects on broiler growth performance and cecal microbiota. In this study, the chickens were fed different rates of MOLP (1%, 3%, 5%, 7%, and 9%) instead of the rape seed cake, and the effects of different levels of MOLP on growth performance, carcass characteristics, and cecal microbiota of the broilers were evaluated at two different growth stages (day 28 and day 56). In terms of growth performance, the best results were obtained at the 3% MOLP level in the early stages (p < 0.05). In terms of carcass characteristics, in the early stage, the level of 5% MOLP had the best effect; in the later stage, 5% MOLP also had the best effect. In terms of cecal microbial changes, the alpha diversity analysis revealed that 5% MOLP enhanced the richness and diversity of broiler intestinal flora. At the phylum level, the addition of 5% MOLP adjusted the relative abundance of Firmicutes and Bacteroidetes to a level close to that of the A1 group on day 28, while 5% MOLP significantly reduced the relative abundance of Bacteroidetes (p < 0.05) compared to the A2 group on day 56, and the relative abundance of Firmicutes was still higher in the D2 group than in the A2 group (p < 0.05). At the genus level, MOLP addition consistently and significantly increased the relative abundance of Bacteroides (p < 0.05), except for 3% on day 28 and 1% on day 56. For Oscillospira, increasing MOLP levels in the pre- and post-period resulted in a significant decrease in the relative abundance of Oscillospira (p < 0.05). In conclusion, MOLP helps to enhance growth performance and carcass characteristics and improve the cecal microbial structure of broilers. The recommended rate of MOLP addition for broilers is 5% in both the early and late stages.
Short-Term Survival and Postoperative Complications Rates in Horses Undergoing Colic Surgery: A Multicentre Study
Authors: Spadari Alessandro , Gialletti Rodolfo , Gandini Marco , Valle Emanuela , Cerullo Anna , Cavallini Damiano , Bertoletti Alice , Rinnovati Riccardo , Forni Giulia , Scilimati Nicola , Giusto Gessica
Published: March 2023

The occurrence of colic could be influenced by the characteristics of a population, geographical area, and feeding management. The aim of this study was to report the short-term postoperative complications and survival rates and to identify factors that might affect the outcome of horses that underwent colic surgery in three Italian surgical referral centres. Data of horses subjected to colic surgery in three referral centres (2018–2021) were analysed. Comparisons of the outcomes were performed using a Mann–Whitney or a Chi square test. Areas under the receiver operating characteristic (ROC) curve and multivariable logistic regression analysis were used for parameters that were significant in the previous univariate analysis. The goodness-of-fit of the model was assessed using the Akike information criterion (AIC). Significance was defined as p < 0.05, and odds ratios and 95% confidence intervals were calculated as percentages. A total of 451 horses were included. The survival rate was 68.5% of all of the horses that underwent colic surgery and 80% of the horses surviving anaesthesia. Age, BCS, PCV and TPP before and after surgery, amount of reflux, type of disease, type of lesion, duration of surgery, surgeon’s experience, and amount of intra- and postoperative fluids administered influenced the probability of short-term survival. The multivariate analysis revealed that PCV at arrival, TPP after surgery, and BCS had the highest predictive power. This is the first multicentre study in Italy. The results of this study may help surgeons to inform owners regarding the prognosis of colic surgery.
Vernonia polyanthes Less. (Asteraceae Bercht. & Presl), a Natural Source of Bioactive Compounds with Antibiotic Effect against Multidrug-Resistant Staphylococcus aureus
Authors: Gitirana de Santana Jordana Damasceno , Santos-Mayorga Oscar Alejandro , Florencio Jônatas Rodrigues , Oliveira Mirella Chrispim Cerqueira de , Almeida Luísa Maria Silveira de , Xavier Julianna Oliveira de Lucas , Zimmermann-Franco Danielle Cristina , Macedo Gilson Costa , Ferreira Adriana Lúcia Pires , Sousa Orlando Vieira de , da Silva Filho Ademar Alves , Alves Maria Silvana
Published: March 2023

Vernonia polyanthes is a medicinal plant used to treat many disorders, including infectious diseases. This study investigated the chemical constituents and the antibacterial activity of V. polyanthes leaf rinse extract (Vp-LRE). The chemical characterization of Vp-LRE was established using ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS), and glaucolide A was identified through 1H and 13C nuclear magnetic resonance (NMR) and mass fragmentation. The cytotoxicity was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). The antibacterial activity was assessed by minimal inhibitory concentration and minimal bactericidal concentration. Interactions between ligands and beta-lactamase were evaluated via molecular docking. UHPLC/Q-TOF-MS detected acacetin, apigenin, chrysoeriol, isorhamnetin, isorhamnetin isomer, kaempferide, 3′,4′-dimethoxyluteolin, 3,7-dimethoxy-5,3′,4′-trihydroxyflavone, piptocarphin A and glaucolide A. Vp-LRE (30 µg/mL) and glaucolide A (10 and 20 μg/mL) were cytotoxic against RAW 264.7 cells. Glaucolide A was not active, but Vp-LRE inhibited the Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Escherichia coli, Salmonella Choleraesuis and Typhimurium, with a bacteriostatic effect. The compounds (glaucolide A, 3′,4′-dimethoxyluteolin, acacetin and apigenin) were able to interact with beta-lactamase, mainly through hydrogen bonding, with free energy between −6.2 to −7.5 kcal/mol. These results indicate that V. polyanthes is a potential natural source of phytochemicals with a significant antibiotic effect against MRSA strains.
Impact of Bariatric Surgery on the Stability of the Genetic Material, Oxidation, and Repair of DNA and Telomere Lengths
Authors: Ferk Franziska , Mišík Miroslav , Ernst Benjamin , Prager Gerhard , Bichler Christoph , Mejri Doris , Gerner Christopher , Bileck Andrea , Kundi Michael , Langie Sabine , Holzmann Klaus , Knasmueller Siegfried
Published: March 2023

Obesity causes genetic instability, which plays a key-role in the etiology of cancer and aging. We investigated the impact of bariatric surgery (BS) on DNA repair, oxidative DNA damage, telomere lengths, alterations of antioxidant enzymes and, selected proteins which reflect inflammation. The study was realized with BS patients (n = 35). DNA damage, base oxidation, BER, and NER were measured before and 1 month and 6 months after surgery with the single-cell gel electrophoresis technique. SOD and GPx were quantified spectrophotometrically, malondealdehyde (MDA) was quantified by HPLC. Telomere lengths were determined with qPCR, and plasma proteome profiling was performed with high-resolution mass spectrophotometry. Six months after the operations, reduction of body weight by 27.5% was observed. DNA damage decreased after this period, this effect was paralleled by reduced formation of oxidized DNA bases, a decline in the MDA levels and of BER and NER, and an increase in the telomere lengths. The activities of antioxidant enzymes were not altered. Clear downregulation of certain proteins (CRP, SAA1) which reflect inflammation and cancer risks was observed. Our findings show that BS causes reduced oxidative damage of DNA bases, possibly as a consequence of reduction of inflammation and lipid peroxidation, and indicate that the surgery has beneficial long-term health effects.
A Comprehensive Review of Mechanisms, Predictive Techniques, and Control Strategies of Rockburst
Authors: Waqar Muhammad Faisal , Guo Songfeng , Qi Shengwen
Published: March 2023

Brittle failure, also known as rockburst, is a violent phenomenon that occurs during the excavation of hard rock in areas with high geostress, particularly at great depths. The induced hazards have been a global concern since the first half of the 20th century, leading to extensive efforts to understand the mechanisms and develop effective approaches to predict and control such rock mass failures. To understand the current state of research and future prospects of rockburst, a comprehensive review of past developments has been conducted. Firstly, we briefly summarize different definitions and types of rockburst. Next, we delve into the various technologies and methods employed in rockburst research and analyze the current knowledge on the mechanisms, classifications, and prediction of rockbursts. Then, we examine the existing methods for preventing and controlling these events. Despite the advancements made, the relationship between the behavior of rock masses at different scales, both in the laboratory and real-world conditions, remains an area of challenge. Additionally, the absence of rock mass classifications under high in-situ stress and lack of accuracy in rockburst prediction methods remain persistent issues that need to be addressed. This study delves into cutting-edge rockburst research, illuminating potential ground support strategies and empowering the mining industry in order to unleash innovative solutions for controlling these explosive phenomena.
Novel Insights from Fourier-Transform InfraRed Imaging on the Morpho-Chemical Profile of Human Corpus Callosum
Authors: Belloni Alessia , Montanari Eva , Sagrati Andrea , Lorenzi Teresa , Balloni Aurora , Busardò Francesco Paolo , Notarstefano Valentina , Fabri Mara , Giorgini Elisabetta
Published: March 2023

The corpus callosum (CC) is the largest interhemispheric commissure of the mammalian brain, and it includes axons, cortical neurons, and glial cells. It is mainly composed of myelin, a lipidic sheath which is produced by glial cell membranes; myelin is wrapped up around axons and plays a fundamental role in the fast conduction of neuronal electrical signals. The human CC is divided into various anatomical regions, with different axonal composition, including, from front to back, genu, body or trunk, isthmus, and splenium. Corpus callosum undergoes some alterations not only in the presence of specific physiological and pathological conditions, but also because of aging. For the first time, in the present study a hyperspectral imaging analysis of human corpus callosum was performed. The study, carried out on CC autopsy samples collected from human adult males of different ages, was focused mainly on the genu and splenium regions. By combining Fourier-transform infrared imaging and histological analyses with multivariate and univariate ones, the macromolecular composition of these regions was defined, and age-related alterations in the lipid and protein components were identified.
A Machine Learning Approach to the Non-Invasive Estimation of Continuous Blood Pressure Using Photoplethysmography
Authors: Tarifi Basheq , Fainman Aaron , Pantanowitz Adam , Rubin David M.
Published: March 2023

Blood pressure is an important vital sign that sometimes requires continuous measurement. The current methods include cuff measurements (manual auscultation and oscillometric techniques) for non-continuous measurement and invasive arterial cannulation for continuous measurement. The use of photoplethysmography as a cuffless, non-invasive, and continuous blood pressure measurement system is investigated through the use of four neural networks. These predict the systolic blood pressure, diastolic blood pressure, mean arterial blood pressure, and waveform shape. The models are trained on 890 h of data from 1669 patients in the MIMIC-III database. Feature-trained artificial neural networks predict the systolic blood pressure to 5.26 ± 6.53 mmHg (mean error ± standard deviation), the diastolic blood pressure to 2.96 ± 3.31 mmHg, and the mean arterial pressure to 3.27 ± 3.55 mmHg. These are used to shift and scale the predicted waveform, allowing the waveform prediction neural network to optimise for the wave shape rather than the amplitude. The waveform prediction has 86.4% correlation with the actual arterial blood pressure waveform. All results meet international clinical blood pressure measurement standards and could potentially change how blood pressure is measured in both clinical and research settings. However, more data from healthy individuals and analysis of the models’ biases based on clinical features is required.
Recycled Carbon Fibers with Improved Physical Properties Recovered from CFRP by Nitric Acid
Authors: Sakai Asuka , Kurniawan Winarto , Kubouchi Masatoshi
Published: March 2023

To effectively reuse a large amount of Carbon Fiber Reinforced Plastics (CFRP) waste, the carbon fibers should be able to be recovered without degrading their quality. In this report, we developed a new approach to recover carbon fibers from CFRPs with improved physical properties compared to virgin carbon fibers with an environmentally friendly recycling method using nitric acid. Following the decomposition of the CFRP waste in nitric acid at 80 °C, both recycled carbon fibers and decomposed resin were recovered. The obtained recycled carbon fibers showed 1.4 times higher tensile strength and 2.2 times higher interfacial shear strength to resin compared to virgin carbon fibers. TEM-EDX analysis showed a decrease in the abundance of voids existing in the carbon fiber surface layer and new polar functional groups caused by nitric acid existing inside the voids, leading to increased tensile strength. Furthermore, XPS analysis showed that the interfacial shear strength improved due to the formation of new polar functional groups due to nitric acid. The possibility of applying recycled carbon fibers to CFRP products was shown by elucidating the mechanism that expressed its physical properties during the recycling process, leading to a novel approach to realizing closed-loop recycling.
Smartwatch In-Air Signature Time Sequence Three-Dimensional Static Restoration Classification Based on Multiple Convolutional Neural Networks
Authors: Guo Yuheng , Sato Hiroyuki
Published: March 2023

In-air signatures are promising applications that have been investigated extensively in the past decades; an in-air signature involves gathering datasets through portable devices, such as smartwatches. During the signing process, individuals wear smartwatches on their wrists and sign their names in the air. The dataset we used in this study collected in-air signatures from 22 participants, resulting in a total of 440 smartwatch in-air signature signals. The dynamic time warping (DTW) algorithm was applied to verify the usability of the dataset. This paper analyzes and compares the performances of multiple convolutional neural networks (CNN) and the transformer using median-sized smartwatch in-air signatures. For the four CNN models, the in-air digital signature data were first transformed into visible three-dimensional static signatures. For the transformer, the nine-dimensional in-air signature signals were concatenated and downsampled to the desired length and then fed into the transformer for time sequence signal multi-classification. The performance of each model on the smartwatch in-air signature dataset was thoroughly tested with respect to 10 optimizers and different learning rates. The best testing performance score in our experiment was 99.8514% with ResNet by using the Adagrad optimizer under a 1×10−4 learning rate.
An Experimental Study on Ice Accretion under Bridge Cable in Different Conditions
Authors: Li Wentao , Geng Zhiyuan , Xiao Henglin , Pei Yaoyao , Yang Kang
Published: March 2023

The ice accumulation on the surface of the stay cable of the bridge is a frequently identified problem that threatens the safety of bridge traffic. Therefore, it is important to investigate the ice accumulation on the stay cable under different conditions. To understand the distribution characteristics and variation rules of ice, this study analyzed the effects of ambient temperature, cable inclination angle, and diameter on the ice accumulation of the high-density polyethylene (HDPE) stay cable. The results show that the ambient temperature significantly affects ice formation in the lower part of the stay cable. Low temperature facilitates the formation and growth of icicles, while larger size and inclination angle of the stay cable inhibit the icicle growth. When the inclination angle of the cable is less than 60°, icicles easily form at the bottom of the cable. Smaller-diameter cables are more likely to accumulate icicles at the bottom.
Body Cell Mass from Bioelectrical Impedance Analysis in Patients with Stroke Undergoing Rehabilitation
Authors: Guerrini Alessandro , Siotto Mariacristina , Germanotta Marco , Schirru Mirko , Pavan Arianna , Cipollini Valeria , Insalaco Sabina , Aprile Irene
Published: March 2023

The majority of BIA parameters have been investigated in relation to post-stroke recovery, except for body cell mass (BCM), which measures the body’s cellular components involved in oxygen consumption and nutritional status. The aim of this longitudinal prospective study was to investigate in a cohort of subacute post-stroke patients undergoing rehabilitation the body composition parameters from BIA and the relationship between these parameters, in particular BCM, with both the performance in activity of daily living (ADL), measured by Barthel’s Index at admission (BI T0), and the recovery in ADL, measured by change in BI from baseline (ΔBI). We analyzed 66 patients, before and after a six-week rehabilitation program and we found that patients’ body composition after the rehabilitation protocol did not change. All lean mass and muscle quality parameters measured by BIA are positively correlated with BI T0. Moreover, patients with better recovery had higher values of BCM, such as other lean mass parameters and their related height-adjusted indices. Furthermore, BCM was independently associated both with ADL performance at admission, and most notably with recovery. These results suggest that assessing BCM at admission not only would improve the valuation of the nutritional status in post-stroke patients but would also potentially predict their recovery.
Calamintha incana (Sm.) Helder: A New Phytoextract with In Vitro Antioxidant and Antidiabetic Action
Authors: Althaher Arwa R. , Mastinu Andrea
Published: March 2023

This study aimed to assess the antioxidant and antidiabetic properties of ethanolic phytoextracts of Calamintha incana (Sm.) Helder leaves. Initially, the chemical characterization of the phytocomplex was performed using high-performance liquid chromatography (HPLC)/mass spectrometry (MS). The cytotoxicity of the ethanolic extract was assessed using an MTT assay in HepG2 cells. Subsequently, antioxidant activity was evaluated using a DPPH test. Finally, enzymatic tests with α-amylase, α-glucosidase, pancreatic lipase, and dipeptidyl peptidase IV (DPP-IV) were performed to evaluate their effects on glucose metabolism. The chemical composition of the extract is p-linolenic acid (13.2%), myristic acid (12.1%), and p-cymene (10.5%). The extract demonstrated low toxicity, with none of the tested concentrations inducing 50% cell death. Furthermore, the ethanolic extract revealed potent antioxidant activity using DPPH (IC50 was 35.9 ± 0.7 µg/mL) and reducing power capacity (IC50 was 90.3 ± 0.8 µg/mL). Regarding the antidiabetic activity, the extract caused a significant inhibition of α-amylase, α-glucosidase (IC50 46.3 ± 0.2, 56.8 ± 0.1 μg/mL, respectively), weak inhibition of pancreatic lipase and no notable inhibition of dipeptidyl peptidase IV. In conclusion, C. incana has antioxidant and antidiabetic properties and appears to exert insulin-independent hypoglycemic action.
Geometric Morphometrics and Machine Learning Models Applied to the Study of Late Iron Age Cut Marks from Central Spain
Authors: Maté-González Miguel Ángel , Estaca-Gómez Verónica , Aramendi Julia , Sáez Blázquez Cristina , Rodríguez-Hernández Jesús , Yravedra Sainz de los Terreros José , Ruiz-Zapatero Gonzalo , Álvarez-Sanchís Jesús R.
Published: March 2023

Recently the incorporation of artificial intelligence has allowed the development of valuable methodological advances in taphonomy. Some studies have achieved great precision in identifying the carnivore that produced tooth marks. Additionally, other works focused on human activity have managed to specify what type of tool or raw material was used in the filleting processes identified at the sites. Through the use of geometric morphometrics and machine learning techniques, the present study intends to analyze the cut marks of the Ulaca oppidum (Solosancho, Ávila, Spain) in order to identify the type of tools used during carcass modification. Although the Ulaca oppidum is an Iron Age site, the results suggest that most of the cut marks were produced with flint tools.
Influence of Suction on Capacity of Pile Foundation
Authors: Sagu Aizhan , Satyanaga Alfrendo , Gofar Nurly , Irawan Sonny , Khamitov Ruslan , Moon Sung-Woo , Kim Jong
Published: March 2023

In the common practice of geotechnical design, the state of soil is considered fully saturated, while recent examples show the importance of the consideration of unsaturated conditions as well due to the location of the groundwater table at deeper levels. However, it is problematic to design a foundation and calculate bearing capacity as the internal stress in unsaturated conditions needs to be quantified. The mechanics of unsaturated soils are critical in geotechnical engineering, especially when the case of the foundation design is required. This study aims to investigate the influence of suction on the shaft capacity of pile foundations based on the data obtained from in situ and laboratory testing. The three modified methods (i.e., modified α, β, and λ) were implemented in this study in order to calculate the shaft capacity of the pile foundation incorporating unsaturated soil mechanics principles. In addition, the effect of varying matric suction on shaft capacity was discussed. The results of obtained changes show the importance of rain infiltration in the design of piles in unsaturated conditions. It can be concluded that the most conservative method is found to be the modified β method in designing pile foundations incorporating the suction changes due to rainwater infiltration. However, cost optimization shall be considered while choosing the method for the design. As for the increase in GWT, the modified λ method depicted the most conventional behavior. The possible rain infiltration decreases matric suction, mostly in the upper layers.