bluuebunny/crossref_metadata_2025 huggingface dataset:
Performance and Stability of Wet-Milled CoAl2O4, Ni/CoAl2O4, and Pt,Ni/CoAl2O4 for Soot Combustion
Authors: Álvarez-Docio Carmen M. , Portela Raquel , Reinosa Julián J. , Rubio-Marcos Fernando , Pascual Laura , Fernández José F.
Published: April 2020

Low-energy wet milling was employed to activate commercial CoAl2O4 spinel and disperse mono- and multimetallic nanoparticles on its surface. This method yielded efficient Pt,Ni catalysts for soot oxidation in simulated diesel exhaust conditions. The characterization and activity results indicated that although Ni/CoAl2O4 was highly active, the presence of Pt was required to obtain a stable Ni(0.25 wt. %),Pt(0.75 wt. %)/CoAl2O4 catalyst under the operating conditions of diesel particulate filters, and that hot spots formation must be controlled to avoid the deactivation of the cobalt aluminate. Our work provides important insight for new design strategies to develop high-efficiency low-cost catalysts. Platinum-containing multimetallic nanostructures could efficiently reduce the amount of the costly, but to date non-replaceable, Pt noble metal for a large number of industrially important catalytic processes.
Tunable Composition Aqueous-Synthesized Mixed-Phase TiO2 Nanocrystals for Photo-Assisted Water Decontamination: Comparison of Anatase, Brookite and Rutile Photocatalysts
Authors: Chalastara Konstantina , Guo Fuqiang , Elouatik Samir , Demopoulos George P.
Published: April 2020

Mixed-phase nanoTiO2 materials attract a lot of attention as advanced photocatalysts for water decontamination due to their intrinsic structure that allows better photo-excited e−cb-h+vb charge separation, hence improved photocatalytic efficiency. Currently, the best-known mixed-phase TiO2 photocatalyst is P25 with approximate composition 80% Anatase/20% Rutile (A/r). Apart from Anatase (A) and Rutile (R) phases, there is Brookite (B) which has been evaluated less as photocatalyst in mixed-phase nanoTiO2 systems. In this work we present a sustainable solution process to synthesize tunable composition mixed-phase nanotitania photocatalysts in a continuously stirred tank reactor (CSTR) by modulating conditions like pH, CTiCl4 and time. In particular three mixed-phase TiO2 nanomaterials were produced, namely one predominantly anatase with brookite as minor component (A/b), one predominantly brookite with minor component rutile (B/r), and one predominantly rutile with minor component brookite (R/b) and evaluated as photocatalysts in the degradation of methyl orange. The three semiconducting nanomaterials were characterized by XRD and Raman spectroscopy to quantify the phase ratios and subjected to nano-morphological characterization by FE-SEM and TEM/HR-TEM. The new mixed-phase nanoTiO2 materials are shown to be endowed with large specific surface area, ranging from 90–125 m2 g−1, double of that of P25, to be mesoporous and be surface-rich in Ti–OH molecular groups varying from 12%–20% versus 4% for P25. These properties though impact the adsorptive capacity with R/b and B/r removing > 50% of MO but not photocatalytic activity. The latter depends on nanograined mixed-phase structure and not mere assembly of different phase nanoparticles. First-order rate constants reveal essentially equivalent photocatalytic activity for anatase nanocrystals with either rutile (P25) or brookite (this work) domains.
A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos
Authors: Feizbakhsh Omid , Pontheaux Florian , Glippa Virginie , Morales Julia , Ruchaud Sandrine , Cormier Patrick , Roch Fernando
Published: April 2020

The sea urchin embryo provides a valuable system to analyse the molecular mechanisms orchestrating cell cycle progression and mitosis in a developmental context. However, although it is known that the regulation of histone activity by post-translational modification plays an important role during cell division, the dynamics and the impact of these modifications have not been characterised in detail in a developing embryo. Using different immuno-detection techniques, we show that the levels of Histone 3 phosphorylation at Threonine 3 oscillate in synchrony with mitosis in Sphaerechinus granularis early embryos. We present, in addition, the results of a pharmacological study aimed at analysing the role of this key histone post-translational modification during sea urchin early development.
Diurnal Rhythmicity of Autophagy Is Impaired in the Diabetic Retina
Authors: Qi Xiaoping , Mitter Sayak K. , Yan Yuanqing , Busik Julia V , Grant Maria B , Boulton Michael E
Published: April 2020

Retinal homeostasis is under both diurnal and circadian regulation. We sought to investigate the diurnal expression of autophagy proteins in normal rodent retina and to determine if this is impaired in diabetic retinopathy. C57BL/6J mice and Bio-Breeding Zucker (BBZ) rats were maintained under a 12h/12h light/dark cycle and eyes, enucleated over a 24 h period. Eyes were also collected from diabetic mice with two or nine-months duration of type 1 diabetes (T1D) and Bio-Breeding Zucker diabetic rat (BBZDR/wor rats with 4-months duration of type 2 diabetes (T2D). Immunohistochemistry was performed for the autophagy proteins Atg7, Atg9, LC3 and Beclin1. These autophagy proteins (Atgs) were abundantly expressed in neural retina and endothelial cells in both mice and rats. A differential staining pattern was observed across the retinas which demonstrated a distinctive diurnal rhythmicity. All Atgs showed localization to retinal blood vessels with Atg7 being the most highly expressed. Analysis of the immunostaining demonstrated distinctive diurnal rhythmicity, of which Atg9 and LC3 shared a biphasic expression cycle with the highest level at 8:15 am and 8:15 pm. In contrast, Beclin1 revealed a 24-h cycle with the highest level observed at midnight. Atg7 was also on a 24-h cycle with peak expression at 8:15am, coinciding with the first peak expression of Atg9 and LC3. In diabetic animals, there was a dramatic reduction in all four Atgs and the distinctive diurnal rhythmicity of these autophagy proteins was significantly impaired and phase shifted in both T1D and T2D animals. Restoration of diurnal rhythmicity and facilitation of autophagy protein expression may provide new treatment strategies for diabetic retinopathy.
Self-Assembled Monolayer Formation on a Dental Orthodontic Stainless Steel Wire Surface to Suppress Metal Ion Elution
Authors: Tamaki Hironori , Abe Shigeaki , Yamagata Shuichi , Yoshida Yasuhiro , Sato Yoshiaki
Published: April 2020

Metal ion elution, including Cr and Ni from dental orthodontic stainless steel, accounts for some allergies. In this study, a self-assembled monolayer (SAM) on a wire surface is proposed for suppressing such elution. This method involves modifying the stainless steel surface using phosphonic acid containing a long alkyl chain. The uncoated and coated wires are immersed in different acidic solutions, and the supernatant is analyzed by inductively coupled plasma mass spectrometry after 1–4 weeks. The results reveal that Cr and Ni ion elution is significantly suppressed by SAM modification. These findings will help in minimizing potential allergens from dental orthodontics.
Noise Reduction Power Stealing Detection Model Based on Self-Balanced Data Set
Authors: Liu Haiqing , Li Zhiqiao , Li Yuancheng
Published: April 2020

In recent years, various types of power theft incidents have occurred frequently, and the training of the power-stealing detection model is susceptible to the influence of the imbalanced data set and the data noise, which leads to errors in power-stealing detection. Therefore, a power-stealing detection model is proposed, which is based on Improved Conditional Generation Adversarial Network (CWGAN), Stacked Convolution Noise Reduction Autoencoder (SCDAE) and Lightweight Gradient Boosting Decision Machine (LightGBM). The model performs Generation- Adversarial operations on the original unbalanced power consumption data to achieve the balance of electricity data, and avoids the interference of the imbalanced data set on classifier training. In addition, the convolution method is used to stack the noise reduction auto-encoder to achieve dimension reduction of power consumption data, extract data features and reduce the impact of random noise. Finally, LightGBM is used for power theft detection. The experiments show that CWGAN can effectively balance the distribution of power consumption data. Comparing the detection indicators of the power-stealing model with various advanced power-stealing models on the same data set, it is finally proved that the proposed model is superior to other models in the detection of power stealing.
Their American Dream
Authors: Davis Danné E.
Published: April 2020

Centuries before W.E.B. DuBois named the colorline—i.e., racism—as the problem of the 20th century, skin color stratification was a persistent phenomenon. In 1983 Black feminist, scholar, and Pulitzer Prize winning author Alice Walker termed “colorism” as “prejudicial or preferential treatment of same-race people based solely on their [skin] color”. Using the tools of genealogy, I conducted a critical family history of my parents, Lem and Mae’s, pursuit of their American Dream. Such exploration digs deep to decipher the nexuses of a family’s evolution. Dr. Maya Angelou routinely shared stories about her past to impart the importance of embracing one’s history. For my parents, the American Dream meant opportunity, which included home ownership. Their American Dream began as African Americans in the United States’ Jim Crow south. Lem was a light-skinned man; Mae a dark-complexion woman. They met, married, and bought a small home in segregated Columbia, South Carolina. Bearing the cloak of oppression, my parents joined millions of southern Blacks in the Great Migration relocating to northern cities—my parents landed in Boston, Massachusetts. Throughout their journey, Lem and Mae reached back to their ancestors, and drew from within themselves to improve their circumstances.
Reverberating Historical Privilege of a “Middling” Sort of Settler Family
Authors: Bell Avril
Published: April 2020

Critical family history illuminates societal relations of inequality through focusing on the experiences and trajectories of particular families. Here, I focus on unequal relations between white settler colonizers and indigenous communities within Aotearoa, New Zealand. I use data gathered from family wills and archival research to sketch aspects of the economic privilege of branches of my own ancestral families in contrast to the economic dispossession and injustices faced by the Māori communities alongside whom they lived. The concept of historical privilege forms the analytic basis of this exploration, beginning with the founding historical windfalls experienced by the Bell and Graham families through their initial acquisition of Māori lands and the parallel historical trauma experienced by Māori at the loss of these lands. I then explore how these windfalls and traumas underpinned the divergent economic trajectories on both sides of this colonial relationship, touching on issues of family inheritance and structural and symbolic privilege. Neither the Bells nor the Grahams accumulated significant wealth, but the stories of such “middling” families are helpful in illuminating mechanisms of historical privilege that we inheritors of such privilege find it difficult to “see” or remember.
Combining Experimental Evolution and Genomics to Understand How Seed Beetles Adapt to a Marginal Host Plant
Authors: Rêgo Alexandre , Chaturvedi Samridhi , Springer Amy , Lish Alexandra M. , Barton Caroline L. , Kapheim Karen M. , Messina Frank J. , Gompert Zachariah
Published: April 2020

Genes that affect adaptive traits have been identified, but our knowledge of the genetic basis of adaptation in a more general sense (across multiple traits) remains limited. We combined population-genomic analyses of evolve-and-resequence experiments, genome-wide association mapping of performance traits, and analyses of gene expression to fill this knowledge gap and shed light on the genomics of adaptation to a marginal host (lentil) by the seed beetle Callosobruchus maculatus. Using population-genomic approaches, we detected modest parallelism in allele frequency change across replicate lines during adaptation to lentil. Mapping populations derived from each lentil-adapted line revealed a polygenic basis for two host-specific performance traits (weight and development time), which had low to modest heritabilities. We found less evidence of parallelism in genotype-phenotype associations across these lines than in allele frequency changes during the experiments. Differential gene expression caused by differences in recent evolutionary history exceeded that caused by immediate rearing host. Together, the three genomic datasets suggest that genes affecting traits other than weight and development time are likely to be the main causes of parallel evolution and that detoxification genes (especially cytochrome P450s and beta-glucosidase) could be especially important for colonization of lentil by C. maculatus.
Marilynne Robinson, Wallace Stevens, and Louis Althusser in the Post/Secular Wilderness: Generosity, Jérémiade, and the Aesthetic Effect
Authors: Muhlestein Daniel
Published: April 2020

In Restless Secularism (2017), Matthew Mutter points out that Wallace Stevens described three related techniques that could be used to attempt to purge secular life of its religious residue: adaptation, substitution, and elimination. Marilynne Robinson pushes back against such secularizing strategies by employing three related techniques of her own: negotiation, grafting, and invitation. She does so to attempt to bridge the gap between religious and humanistic perspectives and—in the process—mounts a spirited defense of religious faith and practice. Robinson uses a fourth technique as well: jérémiade. In its usual sacred form, jérémiade is a lamentation that denounces self-righteousness, religious hypocrisy, and social injustice. Much of what Robinson says about the Christian Right is essentially jérémiade. Robinson’s critique of parascientists is jérémiade as well, although its grounding assumptions are secular rather than sacred. While Robinson’s jérémiades against the Christian Right and against parascientists are effective in isolation, in aggregate they sometimes undercut her more generous and inclusive attempts at negotiation, grafting, and invitation. This may be because Robinson’s essays do not undergo the moderating influence of what Louis Althusser called the aesthetic effect of art, which in Housekeeping (1980), Gilead (2004), Home (2008), and Lila (2014) helps counterbalance the flashes of anger and tendencies toward judgement that periodically surface elsewhere in Robinson’s work. Taking into account the presence—or absence—of the aesthetic effect in Robinson’s work helps explain the sometimes startling differences between Robinson’s fiction and nonfiction and helps provides a new perspective from which to rethink two of the most influential postsecular readings of Robinson’s work to date: Amy Hungerford’s Postmodern Belief (2010) and Christopher Douglas’s If God Meant to Interfere (2016).
Citizens’ Spatial Footprint on Twitter—Anomaly, Trend and Bias Investigation in Istanbul
Authors: Gulnerman Ayse Giz , Karaman Himmet , Pekaslan Direnc , Bilgi Serdar
Published: April 2020

Social media (SM) can be an invaluable resource in terms of understanding and managing the effects of catastrophic disasters. In order to use SM platforms for public participatory (PP) mapping of emergency management activities, a bias investigation should be undertaken with regard to the data related to the study area (urban, regional or national, etc.) to determine the spatial data dynamics. Thus, such determinations can be made on how SM can be used and interpreted in terms of PP. In this study, the city of Istanbul was chosen for social media data research area, as it is one of the most crowded cities in the world and expecting a major earthquake. The methodology for the data investigation is: 1. Obtain data and engage sampling, 2. Identify the representation and temporal biases in the data and normalize it in response to representation bias, 3. Identify general anomalies and spatial anomalies, 4. Manipulate the trend of the dataset with the discretization of anomalies and 5. Examine the spatiotemporal bias. Using this bias investigation methodology, citizen footprint dynamics in the city were determined and reference maps (most likely regional anomaly maps, representation maps, time-space bias maps, etc.) were produced. The outcomes of the study can be summarized in four steps. First, highly active users generate the majority of the data and removing this data as a general approach within a pseudo-cleaning process means concealing a large amount of data. Second, data normalization in terms of activity levels, changes the anomaly outcome resulting from diverse representation levels of users. Third, spatiotemporally normalized data present strong spatial anomaly tendency in some parts of the central area. Fourth, trend data is dense in the central area and the spatiotemporal bias assessments show the data density varies in terms of the time of day, day of week and season of the year. The methodology proposed in this study can be used to extract the unbiased daily routines of the social media data of the regions for the normal days and this can be referred for the emergency or unexpected event cases to detect the change or impacts.
A Non-Invasive Tool for Real-Time Measurement of Sulfate in Living Cells
Authors: Fatima Urooj , Okla Mohammad K. , Mohsin Mohd , Naz Ruphi , Soufan Walid , Al-Ghamdi Abdullah A. , Ahmad Altaf
Published: April 2020

Sulfur (S) is an essential element for all forms of life. It is involved in numerous essential processes because S is considered as the primary source of one of the essential amino acids, methionine, which plays an important role in biological events. For the control and regulation of sulfate in a metabolic network through fluxomics, a non-invasive tool is highly desirable that opens the door to monitor the level of the sulfate in real time and space in living cells without fractionation of the cells or tissue. Here, we engineered a FRET (fluorescence resonance energy transfer) based sensor for sulfate, which is genetically-encoded and named as FLIP-SP (Fluorescent indicator protein for sulfate). The FLIP-SP can measure the level of the sulfate in live cells. This sensor was constructed by the fusion of fluorescent proteins at the N- and C-terminus of sulfate binding protein (sbp). The FLIP-SP is highly specific to sulfate, and showed pH stability. Real-time monitoring of the level of sulfate in prokaryotic and eukaryotic cells showed sensor bio-compatibility with living cells. We expect that this sulfate sensor offers a valuable strategy in the understanding of the regulation of the flux of sulfate in the metabolic network.
The Imbalance among Oxidative Biomarkers and Antioxidant Defense Systems in Thromboangiitis Obliterans (Winiwarter-Buerger Disease)
Authors: Sharebiani Hiva , Fazeli Bahare , Maniscalco Rosanna , Ligi Daniela , Mannello Ferdinando
Published: April 2020

(1) Background: Thromboangiitis obliterans or Winiwarter-Buerger disease (WBD), is an inflammatory, thrombotic occlusive, peripheral vascular disease, usually occurring in young smokers. The pathophysiological mechanisms underlying the disease are not clearly understood. The aim of this study is to investigate the imbalance between oxidants and antioxidants occurring in these patients. (2) Patients and Methods: In this cross-sectional study, 22 male patients with WBD and 20 healthy male smoking habit matched control group were included. To evaluate the possible sources of oxidative stress, the antioxidant biomarkers, and the markers of lipid peroxidation and protein oxidation, serum samples were analyzed for total oxidative status (TOS), total antioxidant capacity (TAC), myeloperoxidase (MPO), coenzyme Q10 (CoQ10), superoxide dismutase (SOD), glutathione reductase (GR), malondialdehyde (MDA), and protein carbonyl (PC) activity and/or content. (3) Results: The circulating levels of TOS, TAC, and CoQ10 were significantly higher in WBD patients, with respect to healthy smokers as controls. No significant difference was found among the serum level of PC, total cholesterol, MPO, and GR activity in WBD patients and healthy smoker controls. The activity of SOD and the mean serum level of MDA were significantly lower in WBD patients, with respect to healthy smoker controls. (4) Conclusion: Considerably high levels of oxidative stress were detected in WBD patients, which were greater than the antioxidant capacity. The low level of MDA may be associated with the enzymatic degradation of lipid peroxidation products. High levels of CoQ10 and low levels of SOD may be related to a harmful oxidative cooperation, leading to the vasoconstriction of WBD, representing a promising tool to discern possible different clinical risks of this poorly understood peripheral occlusive disease.
Concomitant TP53 Mutation Confers Worse Prognosis in EGFR-Mutated Non-Small Cell Lung Cancer Patients Treated with TKIs
Authors: Canale Matteo , Petracci Elisabetta , Delmonte Angelo , Bronte Giuseppe , Chiadini Elisa , Ludovini Vienna , Dubini Alessandra , Papi Maximilian , Baglivo Sara , De Luigi Nicoletta , Verlicchi Alberto , Chiari Rita , Landi Lorenza , Metro Giulio , Burgio Marco Angelo , Crinò Lucio , Ulivi Paola
Published: April 2020

Background: Non-small cell lung cancer (NSCLC) is the primary cause of cancer-related deaths worldwide. Epidermal Growth Factor Receptor (EGFR)-mutated patients usually benefit from TKIs treatment, but a significant portion show unresponsiveness due to primary resistance mechanisms. We investigated the role of TP53 mutations in predicting survival and response to EGFR-TKIs in EGFR-mutated NSCLC patients, to confirm, on an independent case series, our previous results. Methods: An independent retrospective cohort study was conducted, on a case series of 136 EGFR-mutated NSCLC patients receiving first or second generation TKIs as a first line therapy, and a smaller fraction of patients who acquired the T790M resistance mutation and were treated with third generation TKIs in the second or further line of treatment. TP53 mutations were evaluated in relation to disease control rate (DCR), objective response rate (ORR), progression-free survival (PFS) and overall survival (OS) of the patients. Results: Forty-two patients (30.9%) showed a TP53 mutation. Considered together, TP53 mutations had no significant impact on time-to-event endpoints. Considering the different TP53 mutations separately, exon 8 mutations confirmed their negative effect on PFS (HR 3.16, 95% 1.59–6.28, p = 0.001). In patients who developed the T790M resistance mutation, treated with third generation TKIs, the TP53 exon 8 mutations predicted worse PFS (even though not statistically significant), and OS (HR 4.86, 95% CI: 1.25–18.90, p = 0.023). Conclusions: TP53 exon 8 mutations confirmed their negative prognostic impact in patients treated with first and second generation TKIs and demonstrated a role in affecting clinical outcome in patients treated with third generation TKIs.
Microstructures and Phases of Ytterbium Silicate Coatings Prepared by Plasma Spray-Physical Vapor Deposition
Authors: Xiao Jie , Guo Qian , Wei Liangliang , He Wenting , Guo Hongbo
Published: April 2020

Ytterbium silicate coatings were deposited on SiCf/SiC ceramics matrix composite (CMC) substrates by plasma spray-physical vapor deposition (PS-PVD), and the microstructures and phase constituents of the coatings were studied. The results show that the Yb2SiO5 coating prepared with high power and low pressure (65 kW/2 mbar) had quasi-columnar structure, mainly deposited from the vapor phase, whereas the coating prepared with low power and high pressure (40 kW/10 mbar) had a typical layered structure, mainly deposited from the liquid phase. The deposition efficiency of parameter A (~2 μm/min) was also significantly lower than that of parameter B (~20 μm/min). After annealing at 1300 °C for 20 h, the coating prepared by 65 kW/2 mbar was mainly composed of ytterbium disilicate phase (77.2 wt %). The coating also contained some silicon-rich phase. The coating prepared by 40 kW/10 mbar basically consisted of ytterbium monosilicate (63.6 wt %). In addition, a small amount of silicon-rich phase and ytterbium-rich phase were also present in the coating. Accompanied with calculation results by the FactSage software, the cause of deviations in phase compositions was analyzed.
Magnetic Proximity Sensor Based on Magnetoelectric Composites and Printed Coils
Authors: Pereira Nélson , Lima Ana Catarina , Correia Vitor , Peřinka Nikola , Lanceros-Mendez Senentxu , Martins Pedro
Published: April 2020

Magnetic sensors are mandatory in a broad range of applications nowadays, being the increasing interest on such sensors mainly driven by the growing demand of materials required by Industry 4.0 and the Internet of Things concept. Optimized power consumption, reliability, flexibility, versatility, lightweight and low-temperature fabrication are some of the technological requirements in which the scientific community is focusing efforts. Aiming to positively respond to those challenges, this work reports magnetic proximity sensors based on magnetoelectric (ME) polyvinylidene fluoride (PVDF)/Metglas composites and an excitation-printed coil. The proposed magnetic proximity sensor shows a maximum resonant ME coefficient (α) of 50.2 Vcm−1 Oe−1, an AC linear response (R2 = 0.997) and a maximum voltage output of 362 mV, which suggests suitability for proximity-sensing applications in the areas of aerospace, automotive, positioning, machine safety, recreation and advertising panels, among others.
Numerical Study on the Fatigue Limit of Metallic Glasses under Cyclic Tension-Compression Loading
Authors: Yan Jinfeng , Meng Wenjun , Chen Zhi , Guo Hong , Yan Xianguo
Published: April 2020

Numerical study was performed to determine the fatigue limit of metallic glass under tension-compression cyclic loading. A revised free-volume theory which considers the hydrostatic stress was utilized to make the predictions. Systematical simulations showed that a higher strain amplitude is prone to making the sample completely damaged earlier. However, lower strain fluctuations could result in a longer fatigue life. Shear banding evolution history described by free-volume localization could reasonably explain the mechanical responses of different samples. In addition, compressive loading could give rise to a higher stress than that under tensile loading because of hydrostatic stress contribution. In the end, a correlation between fatigue life and applied strain amplitude was plotted which could supply a guidance for designing the engineering application of metallic glass under periodic loading.
Geometric Properties and Algorithms for Rational q-Bézier Curves and Surfaces
Authors: Delgado Jorge , Peña J. M.
Published: April 2020

In this paper, properties and algorithms of q-Bézier curves and surfaces are analyzed. It is proven that the only q-Bézier and rational q-Bézier curves satisfying the boundary tangent property are the Bézier and rational Bézier curves, respectively. Evaluation algorithms formed by steps in barycentric form for rational q-Bézier curves and surfaces are provided.
A Functional Data Analysis for Assessing the Impact of a Retrofitting in the Energy Performance of a Building
Authors: Martínez Comesaña Miguel , Martínez Mariño Sandra , Eguía Oller Pablo , Granada Álvarez Enrique , Erkoreka González Aitor
Published: April 2020

There is an increasing interest in reducing the energy consumption in buildings and in improving their energy efficiency. Building retrofitting is the employed solution for enhancing the energy efficiency in existing buildings. However, the actual performance after retrofitting should be analysed to check the effectiveness of the energy conservation measures. The aim of this work was to detect and to quantify the impact that a retrofitting had in the electrical consumption, heating demands, lighting and temperatures of a building located in the north of Spain. The methodology employed is the application of Functional Data Analyses (FDA) in comparison with classic mathematical techniques such as the Analysis of Variance (ANOVA). The methods that are commonly used for assessing building refurbishment are based on vectorial approaches. The novelty of this work is the application of FDA for assessing the energy performance of renovated buildings. The study proves that more accurate and realistic results are obtained working with correlated datasets than with independently distributed observations of classical methods. Moreover, the electrical savings reached values of more than 70% and the heating demands were reduced more than 15% for all floors in the building.
A Review on Current Development of Membranes for Oil Removal from Wastewaters
Authors: Bolto Brian , Zhang Jianhua , Wu Xing , Xie Zongli
Published: April 2020

The current situation with the problems associated with the removal of oil from wastewaters by membranes is being explored. Many types of membranes have been investigated—organic polymers, inorganic or ceramic species and hybrids of the two. Polymeric membranes can be designed to facilitate the passage of oil, but the more successful approach is with hydrophilic types that encourage the passage of water. Ceramic membranes have an advantage here as they are less often irreversibly fouled and give a higher recovery of oil, with a lower flux decline. Furthermore, they can be cleaned up by a simple heating procedure. More attention should be given to understanding the mechanism of fouling so that operating conditions can be optimised to further reduce fouling and further decrease the flux decline, as well as assisting in the design of antifouling membranes. Another obstacle to ceramic membrane use is the high cost of manufacture. Cheaper starting materials such as clays have been surveyed.
Improved Performance of Polysulfone Ultrafiltration Membrane Using TCPP by Post-Modification Method
Authors: Jia Yuandong , Sun Shuangqing , Li Shunshun , Wang Zhikun , Wen Fushan , Li Chunling , Matsuyama Hideto , Hu Songqing
Published: April 2020

Ultrafiltration (UF) membranes have found great application in sewage purification and desalination due to their high permeation flux and high rejection rate for contaminants under low-pressure conditions, but the flux and antifouling ability of UF membranes needs to be improved. Tetrakis (4-carboxyphenyl) porphyrin (TCPP) has good hydrophilicity, and it is protonated under strongly acidic conditions and then forms strong hydrogen bonds with N, O and S, so that the TCPP would be well anchored in the membrane. In this work, NaHCO3 was used to dissolve TCPP and TMC (trimesoyl chloride) was used to produce a strong acid. Then, TCPP was modified in a membrane with a different rejection rate by a method similar to interfacial polymerization. Performance tests of TCPP/polysulfone (PSf) membranes show that for the membrane with a high BSA (bovine serum albumin) rejection, when the ratio of NaHCO3 to TCPP is 16:1 (wt.%), the pure water flux of membrane Z1 16:1 is increased by 34% (from 455 to 614 Lm−2h−1bar−1) while the membrane retention was maintained above 95%. As for the membrane with a low BSA rejection, when the ratio of NaHCO3 to TCPP was 32:1, the rejection of membrane B2 32:1 was found to increase from 81% to 96%. Although the flux of membrane B2 32:1 decreased, it remained at 638 Lm−2h−1bar−1, which is comparable to the reported polymer ultrafiltration membrane. The above dual results are thought to be attributed to the synergistic effect of protonated TCPP and NaHCO3, where the former increases membrane flux and the latter increases the membrane rejection rate. This work provides a way for the application of porphyrin and porphyrin framework materials in membrane separation.
Effect of Overflow Pipe on the Internal Flow Fields and Separation Performance of W-Shaped Hydrocyclones
Authors: Jiang Lanyue , Liu Peikun , Yang Xinghua , Zhang Yuekan , Li Xiaoyu , Zhang Yulong , Wang Hui
Published: April 2020

The entrainment of fine particles in underflow of a grinding-classification hydrocyclone can cause ore overgrinding, which will lead to reductions in both metal recovery and ball mill throughput. To address this problem, this paper proposed a W-shaped hydrocyclone that can effectively reduce underflow fine particle entrainment. Experimental tests and numerical simulations were employed to deeply investigate overflow pipe diameter influence on the separation performance and internal flow field of W-shaped hydrocyclones. The effects of overflow pipe diameter on air core shape, velocity field, pressure field, and separation performance were studied. The results revealed that as the diameter of the overflow pipe increased, air core gradually stabilized, and air core diameter gradually increased. The diameter of stabilized air core was approximately 45% to 55% of overflow pipe diameter. As overflow pipe diameter increased, hydrocyclone pressure drop decreased, energy consumption was reduced, the tangential velocity decreased, outer vortex axial velocity did not change significantly, and inner vortex axial velocity gradually increased. At the same time, zero-velocity points gradually moved outward, and the inner vortex region expanded. By the increase of overflow pipe diameter, both the underflow yield and split ratio gradually decreased, the coarse particle content in the overflow product increased, and the fine particle content in the underflow product gradually decreased.
Predictors of Response to Exclusive Enteral Nutrition in Newly Diagnosed Crohn´s Disease in Children: PRESENCE Study from SEGHNP
Authors: Moriczi Melinda , Pujol-Muncunill Gemma , Martín-Masot Rafael , Jiménez Treviño Santiago , Segarra Cantón Oscar , Ochoa Sangrador Carlos , Peña Quintana Luis , González Santana Daniel , Rodríguez Martínez Alejandro , Rosell Camps Antonio , Armas Honorio , Barrio Josefa , González de Caldas Rafael , Rodríguez Salas Mónica , Balmaseda Serrano Elena , Donat Aliaga Ester , Bodas Pinedo Andrés , Vaquero Sosa Esther , Vecino López Raquel , Solar Boga Alfonso , Moreno Álvarez Ana , Sánchez Sánchez César , Tolín Hernani Mar , Gutiérrez Junquera Carolina , Martinón Torres Nazareth , Leis Trabazo María Rosaura , Eizaguirre Francisco Javier , García Peris Mónica , Medina Benítez Enrique , Fernández Caamaño Beatriz , Vegas Álvarez Ana María , Crespo Valderrábano Laura , Alonso Vicente Carmen , Rubio Santiago Javier , Galera-Martínez Rafael , García-Romero Ruth , Ros Arnal Ignacio , Fernández Cebrián Santiago , Lorenzo Garrido Helena , Viada Bris Javier Francisco , Velasco Rodríguez-Belvis Marta , Bartolomé Porro Juan Manuel , Blanco Rodríguez Miriam , Barros García Patricia , Botija Gonzalo , Chicano Marín Francisco José , La Orden Izquierdo Enrique , Crehuá-Gaudiza Elena , Navas-López Víctor Manuel , Martín-de-Carpi Javier
Published: April 2020

Exclusive enteral nutrition (EEN) has been shown to be more effective than corticosteroids in achieving mucosal healing in children with Crohn´s disease (CD) without the adverse effects of these drugs. The aims of this study were to determine the efficacy of EEN in terms of inducing clinical remission in children newly diagnosed with CD, to describe the predictive factors of response to EEN and the need for treatment with biological agents during the first 12 months of the disease. We conducted an observational retrospective multicentre study that included paediatric patients newly diagnosed with CD between 2014–2016 who underwent EEN. Two hundred and twenty-two patients (140 males) from 35 paediatric centres were included, with a mean age at diagnosis of 11.6 ± 2.5 years. The median EEN duration was 8 weeks (IQR 6.6–8.5), and 184 of the patients (83%) achieved clinical remission (weighted paediatric Crohn’s Disease activity index [wPCDAI] < 12.5). Faecal calprotectin (FC) levels (μg/g) decreased significantly after EEN (830 [IQR 500–1800] to 256 [IQR 120–585] p < 0.0001). Patients with wPCDAI ≤ 57.5, FC < 500 μg/g, CRP >15 mg/L and ileal involvement tended to respond better to EEN. EEN administered for 6–8 weeks is effective for inducing clinical remission. Due to the high response rate in our series, EEN should be used as the first-line therapy in luminal paediatric Crohn’s disease regardless of the location of disease and disease activity.
Monoacylglycerol Form of Omega-3s Improves Its Bioavailability in Humans Compared to Other Forms
Authors: Cuenoud Bernard , Rochat Isabelle , Gosoniu Maria , Dupuis Lenaick , Berk Evan , Jaudszus Anke , Mainz Jochen , Hafen Gaudenz , Beaumont Maurice , Cruz-Hernandez Cristina
Published: April 2020

Numerous benefits are attributed to omega-3 fatty acids (OM3) especially in cardiovascular health. However, bioavailability and clinical efficacy depend on numerous factors, including OM3 form, food matrix effects (especially the lipid content of the diet), and metabolic capacity. Here, we show in humans that a “pre-digested” OM3-sn-1(3)-monoacylglycerol lipid structure (OM3-MAG) has a significantly greater absorption at high therapeutic doses (2.9 g/day) than the most commonly OM3-ethyl ester (3.1 g/day) form (used for the treatment of hypertriglyceridemia), and a comparable profile to other pre-digested OM3 free fatty acids (OM3-FFA) structure (3.2 g/day). Nutritional supplement doses of MAG resulted in similar increases in OM3 blood level, compared to OM3 triacylglycerols (OM3-TAG) supplements in obese subjects (1.2 g/day) under low fat diet, and in children with cystic fibrosis (1.0 g/day). These results suggest that both forms of pre-digested OM3-MAG and OM3-FFA are effectively absorbed and re-incorporated effectively into triacylglycerols inside the enterocytes, before being exported into the chylomicrons lipid transport system. The pre-digested OM3-MAG might provide a more effective therapy in severe cardiovascular conditions where high doses of OM3 are required and a low-fat diet is indicated, which limited digestive lipase activity.
A Systematic Review: Is Aedes albopictus an Efficient Bridge Vector for Zoonotic Arboviruses?
Authors: Pereira-dos-Santos Taissa , Roiz David , Lourenço-de-Oliveira Ricardo , Paupy Christophe
Published: April 2020

Mosquito-borne arboviruses are increasing due to human disturbances of natural ecosystems and globalization of trade and travel. These anthropic changes may affect mosquito communities by modulating ecological traits that influence the “spill-over” dynamics of zoonotic pathogens, especially at the interface between natural and human environments. Particularly, the global invasion of Aedes albopictus is observed not only across urban and peri-urban settings, but also in newly invaded areas in natural settings. This could foster the interaction of Ae. albopictus with wildlife, including local reservoirs of enzootic arboviruses, with implications for the potential zoonotic transfer of pathogens. To evaluate the potential of Ae. albopictus as a bridge vector of arboviruses between wildlife and humans, we performed a bibliographic search and analysis focusing on three components: (1) The capacity of Ae. albopictus to exploit natural larval breeding sites, (2) the blood-feeding behaviour of Ae. albopictus, and (3) Ae. albopictus’ vector competence for arboviruses. Our analysis confirms the potential of Ae. albopictus as a bridge vector based on its colonization of natural breeding sites in newly invaded areas, its opportunistic feeding behaviour together with the preference for human blood, and the competence to transmit 14 arboviruses.
Antifungal and Antiparasitic Drug Delivery
Authors: Torrado Juan José , Serrano Dolores R. , Capilla Javier
Published: April 2020

Fungal and parasitic diseases affect more than a billion people across the globe, one-sixth of the world’s population, mostly located in developing countries. The lack of effective and safer treatments combined with a deficient diagnosis lead to serious chronic illness or even death. There is a mismatch between the rate of drug resistance and the development of new medicines. Formulation of antifungal and antiparasitic drugs adapted to different administration routes is challenging, bearing in mind their poor water solubility, which limits their bioavailability and efficacy. Hence, there is an unmet clinical need to develop vaccines and novel formulations and drug delivery strategies that can improve the bioavailability and therapeutic effect by enhancing their dissolution, increasing their chemical potency, stabilising the drug and targeting high concentration of drug to the infection sites. This Editorial regards the ten research contributions presented in the Special Issue “Antifungal and Antiparasitic Drug Delivery”.
Preparation, Physicochemical Properties, and In Vitro Toxicity towards Cancer Cells of Novel Types of Arsonoliposomes
Authors: Zagana Paraskevi , Mourtas Spyridon , Basta Anastasia , Antimisiaris Sophia G.
Published: April 2020

Arsonoliposomes (ARSL) are liposomes that incorporate arsonolipids (ARS) in their membranes. They have demonstrated significant toxicity towards cancer cells, while being less toxic towards normal cells. In this study, we sought to investigate the possibility to prepare novel types of arsonoliposomes (ARSL) by incorporating a lipidic derivative of curcumin (TREG) in their membrane, and/or by loading the vesicles with doxorubicin (DOX). The final aim of our studies is to develop novel types of ARSL with improved pharmacokinetics/targeting potential and anticancer activity. TREG was incorporated in ARSL and their integrity during incubation in buffer and serum proteins was studied by monitoring calcein latency. After evaluation of TREG-ARSL stability, the potential to load DOX into ARSL and TREG-ARSL, using the active loading protocol, was studied. Loading was performed at two temperatures (40 °C and 60 °C) and different time periods of co-incubation (of empty vesicles with DOX). Calculation of DOX entrapment efficiency (%) was based on initial and final drug/lipid ratios. The cytotoxic activity of DOX-ARSL was tested towards B16F10 cells (mouse melanoma cells), LLC (Lewis Lung carcinoma cells), and HEK-293 (Human embryonic kidney cells). Results show that TREG-ARSL have slightly larger size but similar surface charge with ARSL and that they are both highly stable during storage at 4 °C for 56 d. Interestingly, the inclusion of TREG in ARSL conferred increased stability to the vesicles towards disruptive effects of serum proteins. The active-loading protocol succeeded to encapsulate high amounts of DOX into ARSL as well as TREG-LIP and TREG-ARSL, while the release profile of DOX from the novel liposome types was similar to that demonstrated by DOX-LIP. The cytotoxicity study results are particularly encouraging, since DOX-ARSL were less toxic towards the (normal) HEK cells compared to the two cancer cell-types. Furthermore, DOX-ARSL demonstrated lower toxicities (at all concentrations tested) for HEK cells, compared to that of the corresponding mixtures of free DOX and empty ARSL, while the opposite was true for the cancer cells (in most cases). The current results justify further in vivo exploitation of DOX-ARSL, as well as TREGARSL as anticancer therapeutic systems.
Coordinated Localization and Antagonistic Function of NtPLC3 and PI4P 5-Kinases in the Subapical Plasma Membrane of Tobacco Pollen Tubes
Authors: Stenzel Irene , Ischebeck Till , Vu-Becker Linh Hai , Riechmann Mara , Krishnamoorthy Praveen , Fratini Marta , Heilmann Ingo
Published: April 2020

Polar tip growth of pollen tubes is regulated by the membrane phospholipid phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), which localizes in a well-defined region of the subapical plasma membrane. How the PtdIns(4,5)P2 region is maintained is currently unclear. In principle, the formation of PtdIns(4,5)P2 by PI4P 5-kinases can be counteracted by phospholipase C (PLC), which hydrolyzes PtdIns(4,5)P2. Here, we show that fluorescence-tagged tobacco NtPLC3 displays a subapical plasma membrane distribution which frames that of fluorescence-tagged PI4P 5-kinases, suggesting that NtPLC3 may modulate PtdIns(4,5)P2-mediated processes in pollen tubes. The expression of a dominant negative NtPLC3 variant resulted in pollen tube tip swelling, consistent with a delimiting effect on PtdIns(4,5)P2 production. When pollen tube morphologies were assessed as a quantitative read-out for PtdIns(4,5)P2 function, NtPLC3 reverted the effects of a coexpressed PI4P 5-kinase, demonstrating that NtPLC3-mediated breakdown of PtdIns(4,5)P2 antagonizes the effects of PtdIns(4,5)P2 overproduction in vivo. When analyzed by spinning disc microscopy, fluorescence-tagged NtPLC3 displayed discontinuous membrane distribution omitting punctate areas of the membrane, suggesting that NtPLC3 is involved in the spatial restriction of plasma membrane domains also at the nanodomain scale. Together, the data indicate that NtPLC3 may contribute to the spatial restriction of PtdIns(4,5)P2 in the subapical plasma membrane of pollen tubes.
Study on the Pyrolysis Kinetics and Mechanisms of the Tread Compounds of Silica-Filled Discarded Car Tires
Authors: Wang Chuansheng , Zhao Baishun , Tian Xiaolong , Wang Kongshuo , Tian Zhongke , Han Wenwen , Bian Huiguang
Published: April 2020

The disposal of used automobile tires is a major waste concern. Simply stacking tires and allowing them to decompose will harbor breeding mosquitoes that spread viruses, whereas burning them will release acidic and toxic gases. Therefore, one viable option is pyrolysis, where elevated temperatures are used to facilitate the decomposition of a material. However, the lack of theoretical support for pyrolysis technology limits the development of the pyrolysis industry when it comes to discarded tires. The purpose of this research is to put forward a brand-new multi-kinetic research method for studying materials with complex components through the discussion of various kinetic research methods. The characteristic of this kinetic research method is that it is a relatively complete theoretical system and can accurately calculate the three kinetic factors considered during the pyrolysis of multicomponent materials. The results show that the multi-kinetic research method can obtain the kinetic equation and reaction mechanism for the pyrolysis of tires with high accuracy. The pyrolysis process of this compound was divided into two stages, Reaction I and II, where the kinetic equation of Reaction I was f ( α ) = 0.2473 α − 3.0473 , with an activation energy of 155.26 kJ/mol and a pre-exponential factor of 5.88 × 109/min. Meanwhile, the kinetic equation of Reaction II was f ( α ) = 0.4142 ( 1 − α ) [ − ln ( 1 − α ) ] − 1.4143 , while its activation energy was 315.40 kJ/mol and its pre-exponential factor was 7.86 × 1017/min. Furthermore, based on the results of the research analysis, the reaction principles corresponding to Reaction I and Reaction II in the pyrolysis process of this compound were established.