bluuebunny/crossref_metadata_2025 huggingface dataset:
Matjarr Djuyal: How Using Gesture in Teaching Gathang Helps Preschoolers Learn Nouns
Authors: Radley Anjilkurri , Jones Caroline , Hanham Jose , Richards Mark
Published: June 2021

There are important efforts being made to revitalise Aboriginal languages in Australia, which are both pedagogically and culturally appropriate. This research seeks to expand the current knowledge of the effectiveness of gesturing as a teaching strategy for young children learning the Gathang language. An experimental method was used to investigate the effectiveness of gesture by employing a context in which other variables (e.g., other teaching pedagogies) could be held constant. Participants, age range 4–5.2 years, were taught Gathang nouns with gesture and without gesture, alongside verbal and pictorial instruction. After the teaching sessions, each child was assessed for their receptive and expressive knowledge of the Gathang nouns, at two time points, two days after instruction (post-test 1) and one week after (post-test 2). At post-test 2, children had stronger receptive knowledge for words they had learned with gesture than without. These findings contribute to a growing body of research attesting to the effectiveness of gesture for improving knowledge acquisition amongst learners. In the context of Aboriginal language revitalisation, gesture also aligns with traditional teaching practices and offers a relatively low-cost strategy for helping teachers assist their students in acquiring Aboriginal languages.
Nutritional Strategies for Childhood Obesity Prevention
Authors: Fornari Elena , Brusati Marco , Maffeis Claudio
Published: June 2021

Background: Reducing the spread of obesity represents a challenge for clinicians in which obesity prevention plays a key role in achieving this purpose. The aim of this review is to analyze the nutritional interventions that can be implemented to prevent childhood obesity. Methods: Searching PubMed and Cochrane Library between 2019 and 2021. Further searching with no date range for articles selected for their specific relevance in the pediatric area or for their scientific relevance. A total of 871 articles were identified and 90 were included. Results: We organized the results of the selected articles into age groups, and according to the subjects targeted for interventions or to the site of interventions, reserving an in-depth analysis on specific nutritional aspects. Promotion of breastfeeding, reduction of protein content of formulated milks, and diet of the first 12–24 months, involving family and schools in interventions that promote physical activity and healthy diet, are promising strategies for reduction of the risk of obesity. To increase the efficacy of interventions, a multidimensional approach is crucial. Conclusions: A multidimensional approach, which takes into consideration different areas of intervention, is pivotal for childhood obesity prevention. Integrated programs involving several components (nutrition and physical activity at first) at different levels (individual, family, school, and institutional) are crucial.
Assessment of Electrical Resistivity and Oxygen Diffusion Coefficient of Cementitious Materials from Microstructure Features
Authors: Zhou Renzhan , Li Qiang , Wang Jiandong , Zhou Kewen , He Rui , Fu Chuanqing
Published: June 2021

A newly proposed modified non-contact electrical resistivity measurement was used to test the resistivity of concrete and cement mortar. The oxygen diffusion coefficients of concrete and mortar were determined by a gas diffusion measurement, and the capillary porosity of concrete and cement mortar was measured by mercury intrusion porosimetry (MIP) measurement. The obtained electrical resistivity and capillary porosity results were verified with other researchers’ data, the measured electrical resistivity results can be estimated by a simple equation from the capillary porosity results. The obtained oxygen diffusion coefficient results were quantitatively correlated with capillary porosity and electrical resistivity measurement results. The proposed equations can be practically used to assess the electrical resistivity and oxygen diffusion coefficient.
Predicting Mechanical Properties of High-Performance Fiber-Reinforced Cementitious Composites by Integrating Micromechanics and Machine Learning
Authors: Guo Pengwei , Meng Weina , Xu Mingfeng , Li Victor C. , Bao Yi
Published: June 2021

Current development of high-performance fiber-reinforced cementitious composites (HPFRCC) mainly relies on intensive experiments. The main purpose of this study is to develop a machine learning method for effective and efficient discovery and development of HPFRCC. Specifically, this research develops machine learning models to predict the mechanical properties of HPFRCC through innovative incorporation of micromechanics, aiming to increase the prediction accuracy and generalization performance by enriching and improving the datasets through data cleaning, principal component analysis (PCA), and K-fold cross-validation. This study considers a total of 14 different mix design variables and predicts the ductility of HPFRCC for the first time, in addition to the compressive and tensile strengths. Different types of machine learning methods are investigated and compared, including artificial neural network (ANN), support vector regression (SVR), classification and regression tree (CART), and extreme gradient boosting tree (XGBoost). The results show that the developed machine learning models can reasonably predict the concerned mechanical properties and can be applied to perform parametric studies for the effects of different mix design variables on the mechanical properties. This study is expected to greatly promote efficient discovery and development of HPFRCC.
Structure and Properties of Heat-Resistant Alloys NiAl–Cr–Co–X (X = La, Mo, Zr, Ta, Re) and Fabrication of Powders for Additive Manufacturing
Authors: Sanin Vitaliy V. , Kaplansky Yury Yu. , Aheiev Maksym I. , Levashov Evgeny A. , Petrzhik Mikhail I. , Bychkova Marina Ya. , Samokhin Andrey V. , Fadeev Andrey A. , Sanin Vladimir N.
Published: June 2021

The NiAl–Cr–Co–X alloys were produced by centrifugal self-propagating high-temperature synthesis (SHS) casting. The effects of dopants X = La, Mo, Zr, Ta, and Re on combustion, as well as the phase composition, structure, and properties of the resulting cast alloys, have been studied. The greatest improvement in overall properties was achieved when the alloys were co-doped with 15% Mo and 1.5% Re. By forming a ductile matrix, molybdenum enhanced strength characteristics up to the values σucs = 1604 ± 80 MPa, σys = 1520 ± 80 MPa, and εpd = 0.79%, while annealing at T = 1250 ℃ and t = 180 min improved strength characteristics to the following level: σucs = 1800 ± 80 MPa, σys = 1670 ± 80 MPa, and εpd = 1.58%. Rhenium modified the structure of the alloy and further improved its properties. The mechanical properties of the NiAl, ZrNi5, Ni0.92Ta0.08, (Al,Ta)Ni3, and Al(Re,Ni)3 phases were determined by nanoindentation. The three-level hierarchical structure of the NiAl–Cr–Co+15%Mo alloy was identified. The optimal plasma treatment regime was identified, and narrow-fraction powders (fraction 8–27 µm) characterized by 95% degree of spheroidization and the content of nanosized fraction <5% were obtained.
New Volatile Perfluorinated Amidine–Carboxylate Copper(II) Complexes as Promising Precursors in CVD and FEBID Methods
Authors: Madajska Katarzyna , Szymańska Iwona Barbara
Published: June 2021

In the present study, we have synthesised and characterised newly copper(II) complexes with the general formula [Cu2(NH2(NH=)CC2F5)2(µ–O2CRF)4], where RF = CF3, C2F5, C3F7, C4F9. Infrared spectroscopy, mass spectrometry with electron ionisation (EI MS), and density-functional theory (DFT) calculations were used to confirm compounds’ composition and structure. The volatility of the compounds was studied using thermal analysis (TGA), EI MS mass spectrometry, variable temperature infrared spectroscopy (VT IR), and sublimation experiments. Research has revealed that these compounds are the source of metal carriers in the gas phase. The thermal decomposition mechanism over reduced pressure was proposed. TGA studies demonstrated that copper transfer to the gaseous phase occurs even at atmospheric pressure. Two selected complexes [Cu2(NH2(NH=)CC2F5)2(µ–O2CC2F5)4] and [Cu2(NH2(NH=)CC2F5)2(µ–O2CC3F7)4] were successful used as chemical vapour deposition precursors. Copper films were deposited with an evaporation temperature of 393 K and 453 K, respectively, and a decomposition temperature in the range of 573–633 K without the use of hydrogen. The microscopic observations made to investigate the interaction of the [Cu2(NH2(NH=)CC2F5)2(µ–O2CC2F5)4] with the electron beam showed that the ligands are completely lost under transmission electron microscopy analysis conditions (200 keV), and the final product is copper(II) fluoride. In contrast, the beam energy in scanning electron microscopy (20 keV) was insufficient to break all coordination bonds. It was shown that the Cu-O bond is more sensitive to the electron beam than the Cu-N bond.
Colored Tattoo Ink Screening Method with Optical Tissue Phantoms and Raman Spectroscopy
Authors: Sadura Filip , Wróbel Maciej S. , Karpienko Katarzyna
Published: June 2021

Due to the increasing popularity of tattoos among the general population, to ensure their safety and quality, there is a need to develop reliable and rapid methods for the analysis of the composition of tattoo inks, both in the ink itself and in already existing tattoos. This paper presents the possibility of using Raman spectroscopy to examine tattoo inks in biological materials. We have developed optical tissue phantoms mimicking the optical scattering coefficient typical for human dermis as a substitute for an in vivo study. The material employed herein allows for mimicking the tattoo-making procedure. We investigated the effect of the scattering coefficient of the matrix in which the ink is located, as well as its chemical compositions on the spectra. Raman surface line scanning has been carried out for each ink in the skin phantom to establish the spatial gradient of ink concentration distribution. This ensures the ability to detect miniature concentrations for a tattoo margin assessment. An analysis and comparison of the spectra of the inks and the tattooed inks in the phantoms are presented. We recommend the utilization of Raman spectroscopy as a screening method to enforce the tattoo ink safety legislations as well as an early medical diagnostic screening tool.
Evaluation of Rhodamine B Photocatalytic Degradation over BaTiO3-MnO2 Ceramic Materials
Authors: Kuźniarska-Biernacka Iwona , Garbarz-Glos Barbara , Skiba Elżbieta , Maniukiewicz Waldemar , Bąk Wojciech , Antonova Maija , Rebelo Susana L. H. , Freire Cristina
Published: June 2021

Ferroelectric ceramics (BaTiO3_MnO2) with different Mn admixtures were prepared using solid-state synthesis. Elemental analysis, powder X-ray diffraction, scanning electron microscopy, Fourier-transform infrared spectroscopy, and impedance spectroscopy confirmed that the BaTiO3 and MnO2 coexisted in the ceramics. In addition, the high purity and homogeneity of the element distributions in the ceramic samples were confirmed. The adsorptive and photocatalytic properties of the BaTiO3 (reference sample, BTO) and BaTiO3_MnO2 materials (BTO_x, where x is wt.% of MnO2 and x = 1, 2 or 3, denoted as BTO_1, BTO_2 and BTO_3, respectively) were evaluated using Rhodamine B (RhB) as the model dye in a photocatalytic chamber equipped with a UV lamp (15 W) in the absence of additional oxidants and (co)catalysts. No adsorption of RhB dye was found for all the materials during 360 min (dark experiment). All samples were photocatalytically active, and the best results were observed for the BTO_3 material, where RhB was 70% removed from aqueous solution during 360 min of irradiation. The photodegradation of RhB in the presence of MnO2-modified BTO ceramics followed a pseudo-first order model and the rate constant of BTO_3 was about 10 times higher than that of BTO, 2 times that of BTO_2, and 1.5 times that of BTO_1. The photocatalysts could be successfully reused after thermal activation.
Thermal, Spectroscopy and Luminescent Characterization of Hybrid PMMA/Lanthanide Complex Materials
Authors: Gil-Kowalczyk Małgorzata , Łyszczek Renata , Jusza Anna , Piramidowicz Ryszard
Published: June 2021

Novel hybrid materials based on the poly(methyl methacrylate) (PMMA) matrix and lanthanide(III) carboxylates Eu:2,6-DClB and Tb:2,6-DClB were synthesized and carefully analyzed in the context of their potential application in optically active polymer-based optical fibers. To determine the usefulness of the obtained materials, a careful thermal, mass spectroscopy, and optical characterization was performed, focusing on the features critical for the technology of optical fiber processing. In addition, the luminescent features of both lanthanide complexes and the resulting hybrid composites were carefully investigated to identify the processes responsible for light emission and to analyze the influence of the PMMA host on light emission intensity and spectral characteristics. The obtained results showed that both lanthanide carboxylate complexes exhibited intense luminescence in the red and green spectral range, typical of europium and terbium dopants, and that those features were well preserved after introducing them into the PMMA polymer. Thermal analysis also proved that introducing the luminescent additives did not significantly affect the thermal properties of both hybrid materials, thus enabling further processing into the form of optical fibers.
Effect of Precursor Deficiency Induced Ca/P Ratio on Antibacterial and Osteoblast Adhesion Properties of Ag-Incorporated Hydroxyapatite: Reducing Ag Toxicity
Authors: Gokcekaya Ozkan , Ergun Celaletdin , Webster Thomas J. , Bahadir Abdurrahman , Ueda Kyosuke , Narushima Takayuki , Nakano Takayoshi
Published: June 2021

Ag-containing hydroxyapatite (HA) can reduce risks associated with bacterial infections which may eventually require additional surgical operations to retrieve a failed implant. The biological properties of HA in such applications are strongly affected by its composition in terms of dopants as well as Ca/P stoichiometry, which can be easily controlled by altering processing parameters, such as precursor concentrations. The objective of this in vitro study was to understand the effect of variations in HA precursor solutions on antibacterial properties against Escherichia coli (E. coli) and for promoting osteoblast (bone-forming cell) adhesion on Ag incorporated HA (AgHA) which has not yet been investigated. For this, two groups of AgHAs were synthesized via a precipitation method by adjusting precursor reactants with a stoichiometric value of 1.67, being either (Ca + Ag)/P (Ca-deficient) or Ca/(P + Ag) (P-deficient), and were characterized by XRD, FTIR, and SEM-EDS. Results showed that Ag+ incorporated into the Ca2+ sites was associated with a corresponding OH− vacancy. Additional incorporation of CO32− into PO43− sites occurred specifically for the P-deficient AgHAs. While antibacterial properties increased, osteoblast adhesion decreased with increasing Ag content for the Ca-deficient AgHAs, as anticipated. In contrast, significant antibacterial properties with good osteoblast behavior were observed on the P-deficient AgHAs even with a lower Ag content, owing to carbonated HA. Thus, this showed that by synthesizing AgHA using P-deficient precursors with carbonate substitution, one can keep the antibacterial properties of Ag in HA while reducing its toxic effect on osteoblasts.
An In Vitro Stereomicroscopic Evaluation of Bioactivity between Neo MTA Plus, Pro Root MTA, BIODENTINE & Glass Ionomer Cement Using Dye Penetration Method
Authors: Karobari Mohmed Isaqali , Basheer Syed Nahid , Sayed Fazlur Rahman , Shaikh Sufiyan , Agwan Muhammad Atif Saleem , Marya Anand , Messina Pietro , Scardina Giuseppe Alessandro
Published: June 2021

The ideal root end filling material should form a tight seal in the root canal by adhering to the cavity walls. Several materials have been used for root end filling. The present study aims to find out and compare the bioactivity of Neo MTA Plus, Pro Root MTA White, BIODENTINE & glass ionomer cement as root end filling materials using 1% methylene blue as tracer. Materials and methods: 80 extracted human permanent maxillary anterior teeth were used in the study. They were divided into four groups. Specimens were sectioned transversely in the cervical area to separate the crown from the root. The root canal was obturated with gutta percha and zinc oxide eugenol sealers. Thereafter, each sample was resected apically by removing 3 mm of the apex and filled with different materials. Samples were kept in buffering solution at 37 °C until the recommended evaluation periods. The specimens were then suspended in 1% methylene blue for 24 h, prior to the analysis. The teeth were then sectioned, and dye penetration was examined, photographed, and evaluated under a stereomicroscope. Results: Vertical dye penetration showed significant differences across different groups. The minimum dye penetration was seen in Neo MTA plus followed by BIODENTINE, Pro Root MTA and maximum in GIC. There was no significant difference in dye penetration between Neo MTA plus and BIODENTINE both at fifteen days and one-month intervals. Conclusion: The present study suggests Neo MTA plus and BIODENTINE should be the preferred material for root end filling.
Lie Symmetry Analysis, Self-Adjointness and Conservation Law for a Type of Nonlinear Equation
Authors: Wang Hengtai , Zou Zhiwei , Shen Xin
Published: June 2021

In the present paper, we mainly focus on the symmetry of the solutions of a given PDE via Lie group method. Meanwhile we transfer the given PDE to ODEs by making use of similarity reductions. Furthermore, it is shown that the given PDE is self-adjoining, and we also study the conservation law via multiplier approach.
Variational Bayesian Learning of SMoGs: Modelling and Their Application to Synthetic Aperture Radar
Authors: Roussos Evangelos
Published: June 2021

We show how modern Bayesian Machine Learning tools can be effectively used in order to develop efficient methods for filtering Earth Observation signals. Bayesian statistical methods can be thought of as a generalization of the classical least-squares adjustment methods where both the unknown signals and the parameters are endowed with probability distributions, the priors. Statistical inference under this scheme is the derivation of posterior distributions, that is, distributions of the unknowns after the model has seen the data. Least squares can then be thought of as a special case that uses Gaussian likelihoods, or error statistics. In principle, for most non-trivial models, this framework requires performing integration in high-dimensional spaces. Variational methods are effective tools for approximate inference in Statistical Machine Learning and Computational Statistics. In this paper, after introducing the general variational Bayesian learning method, we apply it to the modelling and implementation of sparse mixtures of Gaussians (SMoG) models, intended to be used as adaptive priors for the efficient representation of sparse signals in applications such as wavelet-type analysis. Wavelet decomposition methods have been very successful in denoising real-world, non-stationary signals that may also contain discontinuities. For this purpose we construct a constrained hierarchical Bayesian model capturing the salient characteristics of such sets of decomposition coefficients. We express our model as a Dirichlet mixture model. We then show how variational ideas can be used to derive efficient methods for bypassing the need for integration: the task of integration becomes one of optimization. We apply our SMoG implementation to the problem of denoising of Synthetic Aperture Radar images, inherently affected by speckle noise, and show that it achieves improved performance compared to established methods, both in terms of speckle reduction and image feature preservation.
Current Trend of Marine Carbohydrate-Containing Compounds with Medicinal Properties
Authors: Yermak Irina M. , Davydova Viktoria N.
Published: June 2021

Carbohydrates are most abundant biomolecules on Earth and, also, the most complex biomolecules in terms of structure [...]
Old Age Bipolar Disorder—Epidemiology, Aetiology and Treatment
Authors: Arnold Ivan , Dehning Julia , Grunze Anna , Hausmann Armand
Published: June 2021

Data regarding older age bipolar disorder (OABD) are sparse. Two major groups are classified as patients with first occurrence of mania in old age, the so called “late onset” patients (LOBD), and the elder patients with a long-standing clinical history, the so called “early onset” patients (EOBD). The aim of the present literature review is to provide more information on specific issues concerning OABD, such as epidemiology, aetiology and treatments outcomes. We conducted a Medline literature search from 1970–2021 using the MeSH terms “bipolar disorder” and “aged” or “geriatric” or “elderly”. The additional literature was retrieved by examining cross references and by a hand search in textbooks. With sparse data on the treatment of OABD, current guidelines concluded that first-line treatment of OABD should be similar to that for working-age bipolar disorder, with specific attention to side effects, somatic comorbidities and specific risks of OABD. With constant monitoring and awareness of the possible toxic drug interactions, lithium is a safe drug for OABD patients, both in mania and maintenance. Lamotrigine and lurasidone could be considered in bipolar depression. Mood stabilizers, rather than second generation antipsychotics, are the treatment of choice for maintenance. If medication fails, electroconvulsive therapy is recommended for mania, mixed states and depression, and can also be offered for continuation and maintenance treatment. Preliminary results also support a role of psychotherapy and psychosocial interventions in old age BD. The recommended treatments for OABD include lithium and antiepileptics such as valproic acid and lamotrigine, and lurasidone for bipolar depression, although the evidence is still weak. Combined psychosocial and pharmacological treatments also appear to be a treatment of choice for OABD. More research is needed on the optimal pharmacological and psychosocial approaches to OABD, as well as their combination and ranking in an evidence-based therapy algorithm.
Prognostic Value of Systemic Immune-Inflammation Index for Major Adverse Cardiac Events and Mortality in Severe Aortic Stenosis Patients after TAVI
Authors: Tosu Aydin Rodi , Kalyoncuoglu Muhsin , Biter Halil İbrahim , Cakal Sinem , Selcuk Murat , Çinar Tufan , Belen Erdal , Can Mehmet Mustafa
Published: June 2021

Background and objectives: In this study, we aimed to evaluate whether the systemic immune-inflammation index (SII) has a prognostic value for major adverse cardiac events (MACEs), including stroke, re-hospitalization, and short-term all-cause mortality at 6 months, in aortic stenosis (AS) patients who underwent transcatheter aortic valve implantation (TAVI). Materials and Methods: A total of 120 patients who underwent TAVI due to severe AS were retrospectively included in our study. The main outcome of the study was MACEs and short-term all-cause mortality at 6 months. Results: The SII was found to be higher in TAVI patients who developed MACEs than in those who did not develop them. Multivariate Cox regression analysis revealed that the SII (HR: 1.002, 95%CI: 1.001–1.003, p < 0.01) was an independent predictor of MACEs in AS patients after TAVI. The optimal value of the SII for MACEs in AS patients following TAVI was >1.056 with 94% sensitivity and 96% specificity (AUC (the area under the curve): 0.960, p < 0.01). We noted that the AUC value of SII in predicting MACEs was significantly higher than the AUC value of the C-reactive protein (AUC: 0.960 vs. AUC: 0.714, respectively). Conclusions: This is the first study to show that high pre-procedural SII may have a predictive value for MACEs and short-term mortality in AS patients undergoing TAVI.
Anti-Inflammatory and Mineralization Effects of Bromelain on Lipopolysaccharide-Induced Inflammation of Human Dental Pulp Cells
Authors: Hong Joo-Hyun , Kim Mi-Ra , Lee Bin-Na , Oh Won-Mann , Min Kyung-San , Im Yeong-Gwan , Hwang Yun-Chan
Published: June 2021

Background and Objectives: Bromelain is a mixture of protease obtained from pineapple fruits or stems. Even though the biological mechanism of action of bromelain has not been completely understood, it is well known that bromelain possesses anticancer, anti-inflammatory and immunomodulatory effects. This study investigated the anti-inflammatory effects of bromelain on lipopolysaccharide (LPS)-induced human dental pulp cells (hDPCs). Materials and Methods: Cell viability after bromelain treatment was measured using WST-1 assay. We exposed hDPCs to 5 µg/mL of LPS with 2.5 or 5 µg/mL of bromelain. We performed reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assay to detect interleukin-1β, interleukin-6, and interleukin-8 levels. Western blots were used to detect intercellular adhesion molecules-1 (ICAM-1) and vascular cell adhesion molecules-1 (VCAM-1) levels. Immunofluorescence staining and Western blots were used to determine bromelain’s anti-inflammatory mechanism. We also performed alkaline phosphatase and Alizarin red staining to verify mineralization nodule formation. Results: Bromelain at 2.5, 5, 10, or 20 µg/mL did not affect the viability of hDPCs significantly. LPS increased interleukin-1β, interleukin-6, interleukin-8, ICAM-1 and VCAM-1 expression in hDPCs. Bromelain significantly decreased interleukin-1β, interleukin-6, interleukin-8, ICAM-1, and VCAM-1 levels in hDPCs, which were stimulated by LPS. Bromelain treatment significantly reduced p65 phosphorylation in the cytoplasm and the nucleus. It also significantly decreased phosphorylation levels of extracellular signal-related kinases (ERK) and p38 mitogen-activated protein kinases (p38). Bromelain also promoted ALP activity and mineralized nodule formation. Conclusions: Bromelain inhibits the expression of inflammatory cytokines in LPS-stimulated hDPCs. The inhibitory effect of bromelain on inflammatory mediators is related to decreased NF-κB and the MAPK pathway. Therefore, bromelain might have the potential to be used for regenerative endodontics, including vital pulp therapy.
Frostbite and Cold Agglutinin Disease: Coexistence of Two Entities Leading to Poor Clinical Outcomes
Authors: Lorenzo-Villalba Noel , Andres Emmanuel , Guerrero-Niño Javier , Nasco Edward , Cattelan Jessy , Jannot Xavier , Ledoux Marie-Pierre
Published: June 2021

An 83-year-old woman was admitted to the emergency department for a 7-day history of fatigue and progressive cyanosis in the feet and hands after cold exposure despite physical protective measures. Upon arrival, the patient presented with necrotic cutaneous lesions in both hands and distal lower extremities. Upon admission, hemoglobin was 7.6 g/dL and laboratory tests were consistent with cold agglutinin disease (CAD), the presence of monoclonal IgM, and flow cytometry consistent with lymphoplasmacytic lymphoma, but MYD88 L265P mutation was negative. The patient required blood transfusion, resulting in stabilized hemoglobin and a decrease in markers of hemolysis. Treatment with aspirin 250 mg daily and intravenous iloprost 0.5 mL/h was initiated with a poor clinical response at day 4. Amputation was required. Plasma exchange was performed and chemotherapy with rituximab and bendamustine was initiated. The clinical course was marked by further necrosis, prompting discussions regarding an additional amputation that was not performed considering the high surgical risk and refusal by the patient. Supportive treatment was initiated, and the patient expired one month after hospital admission.
A New Direction in Microfluidics: Printed Porous Materials
Authors: Evard Hanno , Priks Hans , Saar Indrek , Aavola Heili , Tamm Tarmo , Leito Ivo
Published: June 2021

In this work, the feasibility of a novel direction for microfluidics is studied by demonstrating a set of new methods to fabricate microfluidic systems. Similarly to microfluidic paper-based analytical devices, porous materials are being used. However, alternative porous materials and different printing methods are used here to give the material the necessary pattern to act as a microfluidic system. In this work, microfluidic systems were produced by the following three separate methods: (1) by curing a porous monolithic polymer sheet into a necessary pattern with photolithography, (2) by screen printing silica gel particles with gypsum, and (3) by dispensing silica gel particles with polyvinyl acetate binder using a modified 3D printer. Different parameters of the printed chips were determined (strength of the printed material, printing accuracy, printed material height, wetting characteristics, repeatability) to evaluate whether the printed chips were suitable for use in microfluidics. All three approaches were found to be suitable, and therefore the novel approach to microfluidics was successfully demonstrated.
Investigation on Atomic Structure and Mechanical Property of Na- and Mg-Montmorillonite under High Pressure by First-Principles Calculations
Authors: Zhao Jian , Cao Yu , Wang Lei , Zhang Hai-Jiang , He Man-Chao
Published: June 2021

Montmorillonite is an important layered phyllosilicate material with many useful physicochemical and mechanical properties, which is widely used in medicine, environmental protection, construction industry, and other fields. In order to a get better understanding of the behavior of montmorillonite under high pressure, we studied its atomic structure, electronic and mechanical properties using density functional theory (DFT), including dispersion corrections, as function of the interlayer Na and Mg cations. At ideal condition, the calculations of lattice constants, bond length, band structure, and elastic modulus of Na- and Mg-montmorillonite are in good agreement with the experimental values. Under high pressure, the lattice constants and major bond lengths decreased with increasing pressure. The calculated electronic properties and band structure show only a slight change under 20 GPa, indicating that the effect of pressure on the electronic properties of Na- and Mg-montmorillonite is weak. The bulk modulus, shear modulus, Young’s modulus, shear wave velocity and compression wave velocity of Na- and Mg-montmorillonite are positively correlated with the external pressure, and the other mechanical parameters have a little change. The calculated studies will be useful to explore experiments in the future from a purely scientific point of view.
Applications of Mössbauer Spectroscopy in Meteoritical and Planetary Science, Part II: Differentiated Meteorites, Moon, and Mars
Authors: Maksimova Alevtina , Goryunov Michael , Oshtrakh Michael
Published: June 2021

Mössbauer (nuclear γ-resonance) spectroscopy is a powerful technique which is actively used in various fields from physics and chemistry to biology and medicine. Rudolf L. Mössbauer, who observed nuclear γ-resonance and published his results in 1958, got a Nobel Prize in physics in 1961 for this discovery. 57Fe is the most widely used nucleus in Mössbauer spectroscopy. Therefore, a large variety of compounds containing iron can be studied by Mössbauer spectroscopy. It is well known that planetary matter contains various iron-bearing phases and minerals. Therefore, the extraterrestrial material from different meteorites, asteroids, and planets can be studied using 57Fe Mössbauer spectroscopy as an additional powerful technique. Two parts of this review consider the results of more than 50 years of experience of Mössbauer spectroscopy applied for the studies of various meteorites, soils and rocks from the Moon and a recent investigation of the Martian surface using two rovers equipped with miniaturized Mössbauer spectrometers. Part I considered the results of Mössbauer spectroscopy of undifferentiated meteorites. Part II discusses the results of Mössbauer spectroscopy of differentiated meteorites formed in asteroids and protoplanets due to matter differentiation, as well as Lunar and Martian matter.
Detecting Impending Malnutrition of (Pre-) Frail Older Adults in Domestic Smart Home Environments
Authors: Friedrich Björn , Bauer Jürgen M. , Hein Andreas , Diekmann Rebecca
Published: June 2021

Malnutrition is a well-known risk factor for deteriorated physical function, disability and loss in independence in older adults. An unintended loss in body weight of more than 5% in 3 months is one indicator for malnutrition. In this study we examined the relationship between meal preparation time, hand grip strength, and body weight in order to map impending nutritional problems using ambient sensors. Data were collected in the domestic environments of 20 (pre-) frail older adults aged 85.75 y (Standard Deviation: 5.19 y) over 10-months of observation. Collecting included physical function and nutritional status of the participants and meal preparation time by a combination of motion and power sensor events. Analysis was done by rank correlation of hand grip strength, body weight, and meal preparation time. Ten participants aged 85.1 years (Standard Deviation: 4.6 y) were included. The results show a significant correlation (≥0.99) of the meal preparation time with the hand grip strength. This result validated the meal preparation time as a suitable measure for analysing the correlation between meal preparation time and body weight, and a significant correlation (≥0.99) found. Hence the meal preparation time could be used as an indicator for malnutrition. However, causalities have to be conducted by further clinical studies.
Magnesium Metabolism in Chronic Alcohol-Use Disorder: Meta-Analysis and Systematic Review
Authors: Vanoni Flora O. , Milani Gregorio P. , Agostoni Carlo , Treglia Giorgio , Faré Pietro B. , Camozzi Pietro , Lava Sebastiano A. G. , Bianchetti Mario G. , Janett Simone
Published: June 2021

Chronic alcohol-use disorder has been imputed as a possible cause of dietary magnesium depletion. The purpose of this study was to assess the prevalence of hypomagnesemia in chronic alcohol-use disorder, and to provide information on intracellular magnesium and on its renal handling. We carried out a structured literature search up to November 2020, which returned 2719 potentially relevant records. After excluding non-significant records, 25 were retained for the final analysis. The meta-analysis disclosed that both total and ionized circulating magnesium are markedly reduced in chronic alcohol-use disorder. The funnel plot and the Egger’s test did not disclose significant publication bias. The I2-test demonstrated significant statistical heterogeneity between studies. We also found that the skeletal muscle magnesium content is reduced and the kidney’s normal response to hypomagnesemia is blunted. In conclusion, magnesium depletion is common in chronic alcohol-use disorder. Furthermore, the kidney plays a crucial role in the development of magnesium depletion.
Targeting HMGB1 in the Treatment of Non-Small Cell Lung Adenocarcinoma
Authors: Anderson Brady , Vue Mary , Gayluak Nya , Brown Sarah Jane , Bemis Lynne T. , Simmons Glenn E.
Published: June 2021

Evidence of immunogenic cell death as a predictor of response to cancer therapy has increased interest in the high molecular group box 1 protein (HMGB1). HMGB1 is a nuclear protein associated with chromatin organization and DNA damage repair. HMGB1 is also a damage-associated molecular pattern (DAMP) protein and promotes proinflammatory signaling in a paracrine and autocrine manner. Extracellular HMGB1 can promote activation of NF-kB and is associated with several chronic inflammatory and autoimmune diseases, including sepsis, rheumatoid arthritis, atherosclerosis, chronic kidney disease, systemic lupus erythematosus (SLE), as well as cancer. In this review, we describe studies that demonstrate the use of deacetylase inhibitors and HMGB1 inhibitors to alter the expression and localization of HMGB1 in cancer cells, with a focus on lung cancer. The drugs described herein are well established and frequently used in human and small mammal studies. The main objective of this review is to summarize the potential benefit of targeting posttranslational modification of HMGB1 to decrease inflammatory signaling in the tumor microenvironment, and perhaps lead to improved response to current immunotherapeutic approaches.
A Review of the Synthetic Strategies toward Dihydropyrrolo[1,2-a]Pyrazinones
Authors: Winant Pieterjan , Horsten Tomas , Gil de Melo Shaiani , Emery Flavio , Dehaen Wim
Published: June 2021

Dihydropyrrolo[1,2-a]pyrazinone rings are a class of heterocycles present in a wide range of bioactive natural products and analogues thereof. As a direct result of their bioactivity, the synthesis of this privileged class of compounds has been extensively studied. This review provides an overview of these synthetic pathways. The literature is covered up until 2020 and is organized according to the specific strategies used to construct the scaffold: fusing a pyrazinone to an existing pyrrole, employing a pyrazinone-first strategy, an array of multicomponent reactions and some miscellaneous reactions.
CBM Performance for Λ Hyperon Directed Flow Measurements in Au + Au Collisions at 12A GeV/c
Authors: Lubynets Oleksii , Selyuzhenkov Ilya , Klochkov Viktor
Published: June 2021

We present the current status of the performance studies of Λ hyperon directed flow measurement with the CBM experiment at the future FAIR facility in Darmstadt. Kalman Filter mathematics is used to reconstruct Λ→pπ− weak decay kinematics, while the Particle Finder Simple package is used to optimize criteria for Λ hyperon candidate selection. Directed flow of Λ hyperons is studied as a function of rapidity, transverse momentum and collision centrality. The effects on flow measurement due to non-uniformity of the CBM detector response in the azimuthal angle, transverse momentum and rapidity are corrected using the QnTools analysis framework.
Pitfalls in Tick and Tick-Borne Pathogens Research, Some Recommendations and a Call for Data Sharing
Authors: Estrada-Peña Agustín , Cevidanes Aitor , Sprong Hein , Millán Javier
Published: June 2021

An understanding of the relationships of ticks and tick-borne pathogens can only be achieved by integrating data from multiple studies. The publication of raw material is a necessary step for wide-area meta-analyses and study design, data collection and reporting require harmonization. This is an opinion paper, not a consensus position, and is open to debate. This work reflects our view about how data should be communicated in mainstream journals. We indicate rules that should be observed in recording weather data, to avoid serendipitous correlations between the density of ticks and climate variables and recommend the inclusion of raw data in reports. We stress the need for standardized methods to collect ticks that cannot be obtained by standard flagging. The reporting of infection rates of pathogens in ticks should avoid conclusions based on pure molecular findings in feeding ticks. Studies demonstrating the vectorial capacity of ticks should not be supported only by molecular surveys of feeding ticks. Vacuous conclusions about vectorial or reservoir status based solely on the finding of genomic material of a pathogen should be discouraged. We stress that phylogenetic studies based on random selection of sequences from GenBank are unsuitable. We firmly support the development of a dedicated server of curated sequences of ticks and pathogens as a standard for future studies.
Observations on the Relationships between Endophytic Metarhizium robertsii, Spodoptera frugiperda (Lepidoptera: Noctuidae), and Maize
Authors: Flonc Brianna , Barbercheck Mary , Ahmad Imtiaz
Published: June 2021

Fungi in the genus Metarhizium are entomopathogens that can establish endophytically inside plants and benefit them through growth promotion and pest suppression. Lab- and greenhouse-based experiments were conducted to examine the effects of endophytic M. robertsii colonization in maize (Zea mays) on fall armyworm (FAW) (Spodoptera frugiperda). Maize seeds were inoculated with M. robertsii conidia, plants were evaluated for endophytic colonization, and then relative growth rate (RGR) and feeding behavior of larval FAW fed leaves from inoculated and uninoculated maize were measured. Endophytic M. robertsii was recovered from 60.5% of inoculated maize. In feeding bioassays, the RGR of larval FAW fed leaves of inoculated maize was no different than the RGR of larvae fed leaves from uninoculated maize. The RGR of larval FAW was positively correlated with the proportion of endophytic colonization of maize leaf and root tissues; however, in feeding assays, FAW larvae demonstrated no preference for consuming leaf tissue from inoculated or uninoculated maize. The proportion of leaf tissue consumed was unrelated to the proportion of M. robertsii-colonization of leaf or root tissue from source plants. We discuss possible reasons why FAW were not affected by endophytic M. robertsii in the context of assay methodology, FAW physiology, and induced maize defenses.
Habitat Characteristics as Potential Drivers of the Angiostrongylus daskalovi Infection in European Badger (Meles meles) Populations
Authors: Nagy Eszter , Benedek Ildikó , Zsolnai Attila , Halász Tibor , Csivincsik Ágnes , Ács Virág , Nagy Gábor , Tari Tamás
Published: June 2021

From 2016 to 2020, an investigation was carried out to identify the rate of Angiostrongylus spp. infections in European badgers in Hungary. During the study, the hearts and lungs of 50 animals were dissected in order to collect adult worms, the morphometrical characteristics of which were used for species identification. PCR amplification and an 18S rDNA-sequencing analysis were also carried out. Global and local spatial autocorrelation methods were used to detect high-rated and low-rated infected animal clusters. We conducted a binary logistic regression analysis along with hierarchical agglomerative clustering to determine the relation between selected biotic and abiotic variables, and the prevalence of an A. daskalovi infection. We found a high prevalence (72%) and moderate mean intensity (14.1) of Angiostrongylus sp. infection. Morphology and sequencing revealed that all animals were infected by A. daskalovi. The results of both spatial autocorrelations suggested that the spatial distribution of infected badgers was more spatially clustered than random. The results of an analysis of the correlation between habitat characteristics and infection showed that the infected animals could be associated with dry and open landscape habitats without extended and connected canopy. It is suggested that the territorial behaviour of badgers and the landscape-directed aggregation of potential intermediate hosts might be the drivers of an A. daskalovi infection.
Genetic Listeria monocytogenes Types in the Pork Processing Plant Environment: From Occasional Introduction to Plausible Persistence in Harborage Sites
Authors: Demaître Niels , Rasschaert Geertrui , De Zutter Lieven , Geeraerd Annemie , De Reu Koen
Published: June 2021

The purpose of this study was to investigate the L. monocytogenes occurrence and genetic diversity in three Belgian pork cutting plants. We specifically aim to identify harborage sites and niche locations where this pathogen might occur. A total of 868 samples were taken from a large diversity of food and non-food contact surfaces after cleaning and disinfection (C&D) and during processing. A total of 13% (110/868) of environmental samples tested positive for L. monocytogenes. When looking in more detail, zone 3 non-food contact surfaces were contaminated more often (26%; 72/278) at typical harborage sites, such as floors, drains, and cleaning materials. Food contact surfaces (zone 1) were less frequently contaminated (6%; 25/436), also after C&D. PFGE analysis exhibited low genetic heterogeneity, revealing 11 assigned clonal complexes (CC), four of which (CC8, CC9, CC31, and CC121) were predominant and widespread. Our data suggest (i) the occasional introduction and repeated contamination and/or (ii) the establishment of some persistent meat-adapted clones in all cutting plants. Further, we highlight the importance of well-designed extensive sampling programs combined with genetic characterization to help these facilities take corrective actions to prevent transfer of this pathogen from the environment to the meat.
Saponins in Cancer Treatment: Current Progress and Future Prospects
Authors: Elekofehinti Olusola Olalekan , Iwaloye Opeyemi , Olawale Femi , Ariyo Esther Opeyemi
Published: June 2021

Saponins are steroidal or triterpenoid glycoside that is distinguished by the soap-forming nature. Different saponins have been characterized and purified and are gaining attention in cancer chemotherapy. Saponins possess high structural diversity, which is linked to the anticancer activities. Several studies have reported the role of saponins in cancer and the mechanism of actions, including cell-cycle arrest, antioxidant activity, cellular invasion inhibition, induction of apoptosis and autophagy. Despite the extensive research and significant anticancer effects of saponins, there are currently no known FDA-approved saponin-based anticancer drugs. This can be attributed to a number of limitations, including toxicities and drug-likeness properties. Recent studies have explored options such as combination therapy and drug delivery systems to ensure increased efficacy and decreased toxicity in saponin. This review discusses the current knowledge on different saponins, their anticancer activity and mechanisms of action, as well as promising research within the last two decades and recommendations for future studies.
Recent Progress in Lipid Nanoparticles for Cancer Theranostics: Opportunity and Challenges
Authors: Bukhari Sarah I. , Imam Syed Sarim , Ahmad Mohammad Zaki , Vuddanda Parameswara Rao , Alshehri Sultan , Mahdi Wael A. , Ahmad Javed
Published: June 2021

Cancer is one of the major leading causes of mortality in the world. The implication of nanotherapeutics in cancer has garnered splendid attention owing to their capability to efficiently address various difficulties associated with conventional drug delivery systems such as non-specific biodistribution, poor efficacy, and the possibility of occurrence of multi-drug resistance. Amongst a plethora of nanocarriers for drugs, this review emphasized lipidic nanocarrier systems for delivering anticancer therapeutics because of their biocompatibility, safety, high drug loading and capability to simultaneously carrying imaging agent and ligands as well. Furthermore, to date, the lack of interaction between diagnosis and treatment has hampered the efforts of the nanotherapeutic approach alone to deal with cancer effectively. Therefore, a novel paradigm with concomitant imaging (with contrasting agents), targeting (with biomarkers), and anticancer agent being delivered in one lipidic nanocarrier system (as cancer theranostics) seems to be very promising in overcoming various hurdles in effective cancer treatment. The major obstacles that are supposed to be addressed by employing lipidic theranostic nanomedicine include nanomedicine reach to tumor cells, drug internalization in cancer cells for therapeutic intervention, off-site drug distribution, and uptake via the host immune system. A comprehensive account of recent research updates in the field of lipidic nanocarrier loaded with therapeutic and diagnostic agents is covered in the present article. Nevertheless, there are notable hurdles in the clinical translation of the lipidic theranostic nanomedicines, which are also highlighted in the present review along with plausible countermeasures.