bluuebunny/crossref_metadata_2025 huggingface dataset:
Systemic Candesartan Treatment Modulates Behavior, Synaptic Protein Levels, and Neuroinflammation in Female Mice That Express Human APOE4
Authors: Scheinman Sarah B. , Zaldua Steve , Dada Adedoyin , Krochmaliuk Kateryna , Dye Katherine , Marottoli Felecia M. , Thatcher Gregory R. J. , Tai Leon M.
Published: February 2021

Evidence suggests that angiotensin receptor blockers (ARBs) could be beneficial for Alzheimer’s disease (AD) patients independent of any effects on hypertension. However, studies in rodent models directly testing the activity of ARB treatment on behavior and AD-relevent pathology including neuroinflammation, Aβ levels, and cerebrovascular function, have produced mixed results. APOE4 is a major genetic risk factor for AD and has been linked to many of the same functions as those purported to be modulated by ARB treatment. Therefore, evaluating the effects of ARB treatment on behavior and AD-relevant pathology in mice that express human APOE4 could provide important information on whether to further develop ARBs for AD therapy. In this study, we treated female and male mice that express the human APOE4 gene in the absence (E4FAD−) or presence (E4FAD+) of high Aβ levels with the ARB prodrug candesartan cilexetil for a duration of 4 months. Compared to vehicle, candesartan treatment resulted in greater memory-relevant behavior and higher hippocampal presynaptic protein levels in female, but not male, E4FAD− and E4FAD+ mice. The beneficial effects of candesartan in female E4FAD− and E4FAD+ mice occurred in tandem with lower GFAP and Iba1 levels in the hippocampus, whereas there were no effects on markers of cerebrovascular function and Aβ levels. Collectively, these data imply that the effects of ARBs on AD-relevant pathology may be modulated in part by the interaction between APOE genotype and biological sex. Thus, the further development of ARBs could provide therapeutic options for targeting neuroinflammation in female APOE4 carriers.
Dysbiotic Gut Microbiota and Dysregulation of Cytokine Profile in Children and Teens With Autism Spectrum Disorder
Authors: Cao Xia , Liu Kevin , Liu Jun , Liu Yen-Wenn , Xu Li , Wang Hua , Zhu Yunhui , Wang Pengfei , Li Zhiwei , Wen Jie , Shen Chen , Li Meng , Nie Zuqing , Kong Xue-Jun
Published: February 2021

Inflammation and the gut-brain axis have been implicated in the pathogenesis of autism spectrum disorders (ASDs). To further understand the relationship between aberrant immune responses and dysbiotic features of the gut microbiome in ASD, we enrolled 45 ASD individuals and 41 healthy control subjects with ages ranging from 2 to 19 years. We found that ASD group subjects have significantly higher plasma levels of IL-2, IL-4, IL-5, IL-6, IL-10, TNF-α, TNF-β, and IFN-γ when compared to healthy controls (FDR-adjusted p < 0.05). The plasma levels of pro-inflammatory cytokines IFN-γ and IL-6 are found to be further associated with several largely pathogenic gut microbiota uniquely detected in subjects with ASD. Furthermore, the ASD gut microbiome is characterized by reduced levels of several beneficial microbiota, including Bacteroides (FDR-adjusted p < 0.01) and Lachnospiraceae (FDR-adjusted p < 0.001). Analysis of Lachnospiraceae family and genus level taxa suggested that relative abundances of such taxa are negatively correlated with pro-inflammatory signaling cytokines IFN-γ and IL-6, particularly in subjects with severe ASD as defined by CARS (p < 0.05). Several largely pathogenic genera are determined to be associated with the pro-inflammatory cytokines IFN-γ and IL-6 (FDR-adjusted p < 0.1). Additionally, IL-4 is significantly negatively correlated with CARS total score (p < 0.05). Based on such results, we propose that the association between the disturbances of specific cytokines and alterations in gut microbiota abundance observed in children and adolescents with ASD provides additional evidence on the induction of aberrant pro-inflammatory mechanisms in ASD and its early diagnosis.
Prognosis and Prophylactic Regional Nodal Irradiation in Breast Cancer Patients With the First Isolated Chest Wall Recurrence After Mastectomy
Authors: Zhao Xu-Ran , Xuan Liang , Yin Jun , Tang Yu , Sun Hui-Ru , Jing Hao , Song Yong-Wen , Jin Jing , Liu Yue-Ping , Fang Hui , Ren Hua , Chen Bo , Tang Yuan , Li Ning , Qi Shu-Nan , Lu Ning-Ning , Yang Yong , Li Ye-Xiong , Sun Bing , Wu Shi-Kai , Wang Shu-Lian
Published: February 2021

Background and Purpose

Optimal radiation target volumes for breast cancer patients with their first isolated chest wall recurrence (ICWR) after mastectomy are controversial. We aimed to analyze the regional failure patterns and to investigate the role of prophylactic regional nodal irradiation (RNI) for ICWR.

Materials and Methods

Altogether 205 patients with ICWR after mastectomy were retrospectively analyzed. Post-recurrence progression-free survival (PFS) and overall survival (OS) rates were calculated by Kaplan-Meier method and the differences were compared with Log-rank test. Competing risk model was used to estimate the subsequent regional recurrence (sRR) and locoregional recurrence (sLRR) rates, and the differences were compared with Gray test.

Results

The 5-year sRR rate was 25.2% with median follow-up of 88.6 months. Of the 52 patients with sRR, 30 (57.7%) recurred in the axilla, 29 (55.8%) in supraclavicular fossa (SC), and five (9.6%) in internal mammary nodes. Surgery plus radiotherapy was independently associated with better sLRR and PFS rates (p<0.001). The ICWR interval of ≤ 4 years was associated with unfavorable sRR (p=0.062), sLRR (p=0.014), PFS (p=0.001), and OS (p=0.005). Among the 157 patients who received radiotherapy after ICWR, chest wall plus RNI significantly improved PFS (p=0.004) and OS (p=0.021) compared with chest wall irradiation alone. In the 166 patients whose ICWR interval was ≤ 4 years, chest wall plus RNI provided the best PFS (p<0.001) and OS (p=0.022) compared with chest wall irradiation alone or no radiotherapy.

Conclusion

Patients with ICWR have a high-risk of sRR in SC and axilla. Chest wall plus RNI is recommended.
Alnus hirsuta (Spach) Rupr. Attenuates Airway Inflammation and Mucus Overproduction in a Murine Model of Ovalbumin-Challenged Asthma
Authors: Lee Ba-Wool , Ha Ji-Hye , Ji Yeongseon , Jeong Seong-Hun , Kim Ju-Hong , Lee Jihye , Park Ji-Young , Kwon Hyung-Jun , Jung Kyungsook , Kim Jong-Choon , Ryu Young-Bae , Lee In-Chul
Published: February 2021

Alnus hirsuta (Spach) Rupr. (AH), a member of the Betulaceae family, is widely used in Eastern Asia of as a source of medicinal compounds for the treatment of hemorrhage, diarrhea, and alcoholism. In this study, we investigated the protective effects of a methanolic extract of AH branches against airway inflammation and mucus production in tumor necrosis factor (TNF)-α-stimulated NCI-H292 cells and in an ovalbumin (OVA)-challenged allergic asthma mouse model. Female BALB/c mice were injected with OVA (40 μg) and aluminum hydroxide (2 mg) on days 0 and 14 to induce allergic airway inflammation. The mice were then challenged with 1% OVA from days 21–23. Mice were treated with AH (50 and 100 mg/kg/day; 2% DMSO) or dexamethasone (positive control; 3 mg/kg/day) from days 18–23. AH treatment effectively attenuated airway resistance/hyperresponsiveness and reduced levels of T helper type 2 (Th2) cytokines, eotaxins, and number of inflammatory cells in bronchoalveolar lavage fluid, and immunoglobulin E in serums of OVA-challenged mice. In histological analysis, AH treatment significantly inhibited airway inflammation and mucus production in OVA-challenged mice. AH treatment downregulated the phosphorylation of I kappa B-alpha, p65 nuclear factor-kappa B (p65NF-κB), and mitogen-activated protein kinases with suppression of mucin 5AC (MUC5AC) in lung tissue. Moreover, AH treatment decreased the levels of pro-inflammatory cytokines and Th2 cytokines, as well as MUC5AC expression, and inhibited the phosphorylation of p65NF-κB in TNF-α-stimulated NCI-H292 cells. These results indicate that AH might represent a useful therapeutic agent for the treatment of allergic asthma.
Combination of Ginsenosides Rb2 and Rg3 Promotes Angiogenic Phenotype of Human Endothelial Cells via PI3K/Akt and MAPK/ERK Pathways
Authors: Choi Ran Joo , Mohamad Zobir Siti Zuraidah , Alexander-Dann Ben , Sharma Nitin , Ma Marcella K.L. , Lam Brian Y.H. , Yeo Giles S.H. , Zhang Weidong , Fan Tai-Ping , Bender Andreas
Published: February 2021

Shexiang Baoxin Pill (SBP) is an oral formulation of Chinese materia medica for the treatment of angina pectoris. It displays pleiotropic roles in protecting the cardiovascular system. However, the mode of action of SBP in promoting angiogenesis, and in particular the synergy between its constituents is currently not fully understood. The combination of ginsenosides Rb2 and Rg3 were studied in human umbilical vein endothelial cells (HUVECs) for their proangiogenic effects. To understand the mode of action of the combination in more mechanistic detail, RNA-Seq analysis was conducted, and differentially expressed genes (DEGs), pathway analysis and Weighted Gene Correlation Network Analysis (WGCNA) were applied to further identify important genes that a play pivotal role in the combination treatment. The effects of pathway-specific inhibitors were observed to provide further support for the hypothesized mode of action of the combination. Ginsenosides Rb2 and Rg3 synergistically promoted HUVEC proliferation and tube formation under defined culture conditions. Also, the combination of Rb2/Rg3 rescued cells from homocysteine-induced damage. mRNA expression of CXCL8, CYR61, FGF16 and FGFRL1 was significantly elevated by the Rb2/Rg3 treatment, and representative signaling pathways induced by these genes were found. The increase of protein levels of phosphorylated-Akt and ERK42/44 by the Rb2/Rg3 combination supports the notion that it promotes endothelial cell proliferation via the PI3K/Akt and MAPK/ERK signaling pathways. The present study provides the hypothesis that SBP, via ginsenosides Rb2 and Rg3, involves the CXCR1/2 CXCL8 (IL8)-mediated PI3K/Akt and MAPK/ERK signaling pathways in achieving its proangiogenic effects.
Dietary Beta-Hydroxy-Beta-Methyl Butyrate Supplementation Affects Growth, Carcass Characteristics, Meat Quality, and Serum Metabolomics Profile in Broiler Chickens
Authors: Tang Zhiyi , Song Bo , Zheng Changbing , Zheng Jie , Yin Yulong , Chen Jiashun
Published: February 2021

This study aimed to explore the effects of beta-hydroxy-beta-methyl butyrate (HMB) on serum metabolic profiles and meat quality of muscles in Wenshi broiler chickens. Birds were fed a basal diet with an additional 0, 0.05, 0.10, or 0.15% HMB, respectively. Results showed that dietary HMB quadratically increased the average daily gain (P = 0.058) and decreased feed:gain (P < 0.05) mainly in the starter phase. At 51 days of age, birds receiving 0.10% HMB diet exhibited less abdominal fat and more breast yield than the control (P < 0.05). Moreover, dietary HMB quadratically decreased the L∗ value and drip loss in selected muscles (P < 0.05) and increased the a∗ value in breast muscle (P < 0.05). Serum metabolome profiling showed that the most differentially abundant metabolites are lipids and lipid-like molecules, including phosphatidylcholines. It was concluded that HMB improved growth performance and meat quality of muscle in broilers.
NO, ROS, RAS, and PVAT: More Than a Soup of Letters
Authors: Barp Clarissa Germano , Bonaventura Daniella , Assreuy Jamil
Published: February 2021

Perivascular adipose tissue (PVAT) has recently entered in the realm of cardiovascular diseases as a putative target for intervention. Notwithstanding its relevance, there is still a long way before the role of PVAT in physiology and pathology is fully understood. The general idea that PVAT anti-contractile effect is beneficial and its pro-contractile effect is harmful is being questioned by several reports. The role of some PVAT important products or systems such as nitric oxide (NO), reactive oxygen species (ROS), and RAS may vary depending on the context, disease, place of production, etc., which adds doubts on how mediators of PVAT anti- and pro-contractile effects are called to action and their final result. This short review will address some points regarding NO, ROS, and RAS in the beneficial and harmful roles of PVAT.
Ovary Signals for Pollen Tube Guidance in Chalazogamous Mangifera indica L.
Authors: Lora Jorge , Perez Veronica , Herrero Maria , Hormaza Jose I.
Published: February 2021

Most flowering plants show porogamy in which the pollen tubes reach the egg apparatus through the micropyle. However, several species show chalazogamy, an unusual pollen tube growth, in which the pollen tubes reach the embryo sac through the chalaza. While ovary signals for pollen tube growth and guidance have been extensively studied in porogamous species, few studies have addressed the process in chalazogamous species such as mango (Mangifera indicaL.), one of the five most important fruit crops worldwide in terms of production. In this study, we characterize pollen–pistil interaction in mango, paying special attention to three key players known to be involved in the directional pollen tube growth of porogamous species such as starch, arabinogalactan proteins (AGPs), and γ-aminobutyric acid (GABA). Starch grains were observed in the style and in the ponticulus at anthesis, but their number decreased 1 day after anthesis. AGPs, revealed by JIM8 and JIM13 antibodies, were homogenously observed in the style and ovary, but were more conspicuous in the nucellus around the egg apparatus. GABA, revealed by anti-GABA antibodies, was specifically observed in the transmitting tissue, including the ponticulus. Moreover, GABA was shown to stimulatein vitromango pollen tube elongation. The results support the heterotrophic growth of mango pollen tubes in the style at the expense of starch, similarly to the observations in porogamous species. However, unlike porogamous species, the micropyle of mango does not show high levels of GABA and starch, although they were observed in the ponticulus and could play a role in supporting the unusual pollen tube growth in chalazogamous species.
Genome-Wide Identification and Characterization of Potato Long Non-coding RNAs Associated With Phytophthora infestans Resistance
Authors: Cao Weilin , Gan Liming , Wang Chenchen , Zhao Xuechen , Zhang Mingyu , Du Jinwen , Zhou Shumei , Zhu Changxiang
Published: February 2021

Long non-coding RNA (lncRNA) is a crucial regulatory mechanism in the plant response to biotic and abiotic stress. However, their roles in potato (Solanum tuberosum L.) resistance to Phytophthora infestans (P. infestans) largely remain unknown. In this study, we identify 2857 lncRNAs and 33,150 mRNAs of the potato from large-scale published RNA sequencing data. Characteristic analysis indicates a similar distribution pattern of lncRNAs and mRNAs on the potato chromosomes, and the mRNAs were longer and had more exons than lncRNAs. Identification of alternative splicing (AS) shows that there were a total of 2491 lncRNAs generated from AS and the highest frequency (46.49%) of alternative acceptors (AA). We performed R package TCseq to cluster 133 specific differentially expressed lncRNAs from resistance lines and found that the lncRNAs of cluster 2 were upregulated. The lncRNA targets were subject to KEGG pathway enrichment analysis, and the interactive network between lncRNAs and mRNAs was constructed by using GENIE3, a random forest machine learning algorithm. Transient overexpression of StLNC0004 in Nicotiana benthamiana significantly suppresses P. infestans growth compared with a control, and the expression of extensin (NbEXT), the ortholog of the StLNC0004 target gene, was significantly upregulated in the overexpression line. Together, these results suggest that lncRNAs play potential functional roles in the potato response to P. infestans infection.
Regulation of Photosystem II Heterogeneity and Photochemistry in Two Cultivars of C4 Crop Sugarcane Under Chilling Stress
Authors: Mathur Sonal , Sunoj Valiaparambil Sebastian John , Elsheery Nabil Ibrahim , Reddy Vangimalla R. , Jajoo Anjana , Cao Kun-Fang
Published: February 2021

In subtropical regions, chilling stress is one of the major constraints for sugarcane cultivation, which hampers yield and sugar production. Two recently released sugarcane cultivars, moderately chilling tolerant Guitang 49 and chilling tolerant Guitang 28, were selected. The experiments were conducted in the controlled environment, and seedlings were exposed to optimum (25°C/15°C), chilling (10°C/5°C), and recovery (25°C/15°C) temperature conditions. PSII heterogeneity was studied in terms of reducing side and antenna size heterogeneity. Under chilling, reducing side heterogeneity resulted in increased number of QB non-reducing centers, whereas antenna side heterogeneity resulted in enhanced number of inactive β centers in both cultivars, but the magnitude of change was higher in Guitang 49 than Guitang 28. Furthermore, in both cultivars, quantum efficiency of PSII, status of water splitting complex, and performance index were adversely affected by chilling, along with reduction in net photosynthesis rate and nighttime respiration and alterations in leaf optical properties. The extents of negative effect on these parameters were larger in Guitang 49 than in Guitang 28. These results reveal a clear differentiation in PSII heterogeneity between differentially chilling tolerant cultivars. Based on our studies, it is concluded that PSII heterogeneity can be used as an additional non-invasive and novel technique for evaluating any type of environmental stress in plants.
Understanding Human Decision Making in an Interactive Landslide Simulator Tool via Reinforcement Learning
Authors: Chaturvedi Pratik , Dutt Varun
Published: February 2021

Prior research has used an Interactive Landslide Simulator (ILS) tool to investigate human decision making against landslide risks. It has been found that repeated feedback in the ILS tool about damages due to landslides causes an improvement in human decisions against landslide risks. However, little is known on how theories of learning from feedback (e.g., reinforcement learning) would account for human decisions in the ILS tool. The primary goal of this paper is to account for human decisions in the ILS tool via computational models based upon reinforcement learning and to explore the model mechanisms involved when people make decisions in the ILS tool. Four different reinforcement-learning models were developed and evaluated in their ability to capture human decisions in an experiment involving two conditions in the ILS tool. The parameters of an Expectancy-Valence (EV) model, two Prospect-Valence-Learning models (PVL and PVL-2), a combination EV-PU model, and a random model were calibrated to human decisions in the ILS tool across the two conditions. Later, different models with their calibrated parameters were generalized to data collected in an experiment involving a new condition in ILS. When generalized to this new condition, the PVL-2 model’s parameters of both damage-feedback conditions outperformed all other RL models (including the random model). We highlight the implications of our results for decision making against landslide risks.
Epidemic Risk Perception, Perceived Stress, and Mental Health During COVID-19 Pandemic: A Moderated Mediating Model
Authors: Li Xiaobao , Lyu Houchao
Published: February 2021

The aim of the present study was to investigate relationships among epidemic risk perception, perceived stress, mental health (depression and anxiety), future time perspective, and confidence in society during the novel coronavirus disease (COVID-19) pandemic in China. Especially, we wonder that whether perceived stress mediates associations between epidemic risk perception and mental health and that whether future time perspective and confidence in society moderate the link between perceived stress and mental health. This cross-sectional study was conducted among 693 Chinese adults aged 18–60 years. The results showed that epidemic risk perception was positively related to perceived stress, depression, and anxiety. The correlations between epidemic risk perception and depression and anxiety were reduced when perceived stress was included, suggesting that perceived stress mediated these relationships. Moreover, the boundary conditions for the associations among perceived stress, depression, and anxiety were found in the study. Specifically, positive future time perspective could buffer the negative effects of perceived stress on depression, and confidence in society could weaken the negative effects of perceived stress on anxiety. Based on these findings, practical guidance and theoretical implications are provided for the public to maintain mental health during COVID-19 pandemic. Limitations and future directions are also discussed.
Not all Perfectionists Are as They Are Assessed: An Investigation of the Psychometric Properties of the Perfectionism Inventory in the Teaching Profession
Authors: Samfira Elena Mirela , Maricuţoiu Laurenţiu P.
Published: February 2021

Perfectionism has been studied for almost 30 years. In the present study, we investigated the internal validity of The Perfectionism Inventory (PI—Hill et al., 2004) in an occupation that encourages perfectionistic tendencies in own behavior or in students' behavior. We collected data from a large sample of schoolteachers (N = 633, 81.18% female, 63.02% from urban areas, 46.66% from secondary schools, mean age = 42.11 years) recruited using a snowball sampling approach, and we analyzed the factor structure of the PI using confirmatory factor analyses. We found that the 8-factor structure of PI provided a reasonable fit root mean square error of approximation [RMSEA = 0.055, 90% CI = (0.053–0.057); SRMR = 0.071]. However, additional analyses revealed problematic divergent validity only in the case of the scales associated with self-evaluative perfectionism, not in the case of the scales associated with conscientious perfectionism. We found that teachers displayed distinguishably different forms of perfectionism only when it referred to own person, not when it referred to perfectionism imposed to others. Based on these findings, we suggested that the PI could provide a useful framework for investigating the role of conscientious-related forms of perfectionism in the development of teacher beliefs regarding their school behavior.
A Promising Candidate to Reliably Index Attentional Bias Toward Alcohol Cues–An Adapted Odd-One-Out Visual Search Task
Authors: Heitmann Janika , Jonker Nienke C. , de Jong Peter J.
Published: February 2021

Attentional bias (AB) has been suggested to contribute to the persistence of substance use behavior. However, the empirical evidence for its proposed role in addiction is inconsistent. This might be due to the inability of commonly used measures to differentiate between attentional engagement and attentional disengagement. Attesting to the importance of differentiating between both components of AB, a recent study using the odd-one-out task (OOOT) showed that substance use was differentially related to engagement and disengagement bias. However, the AB measures derived from the OOOT showed insufficient reliability to be used as a solid measure of individual differences. Therefore, the current study aimed to improve the reliability of the AB measures derived from the OOOT by using more distinct contrast stimuli, adding practice trials, increasing the number of trials, and by having participants perform the task in an alcohol-relevant context. We contrasted the original OOOT with the adapted OOOT (i.e., OOOT-adapt) and assessed AB in low- and high-drinking individuals. Participants were 245 undergraduate students who typically tend to drink either low or high amounts of alcohol. In one condition, AB was measured with the original OOOT in a typical laboratory context, whereas in the other condition, AB was measured with the OOOT-adapt in a bar (i.e., alcohol-relevant) context. The OOOT-adapt showed superior internal consistency, especially for the high-drinking group. Further, specifically the OOOT-adapt differentiated between low- and high-drinking participants showing that high drinkers engaged faster with alcohol cues than did low drinkers. Thus, the OOOT-adapt was found to be a promising candidate to reliably index AB in the context of alcohol use. The OOOT-adapt further showed superior criterion validity as it could differentiate between low- and high-drinking individuals, thereby adding to the evidence that AB might be involved in substance use behavior.
A Pilot Study of Gender Differences in Sexual Arousal of Patients With OCD: The Moderator Roles of Attachment and Contamination Symptoms
Authors: Dèttore Davide , Angelo Nicole Loren , Marazziti Donatella , Mucci Federico , Prestia Davide , Pozza Andrea
Published: February 2021

Sexual arousal is often impaired in patients with obsessive–compulsive disorder (OCD). However, little is known about the factors related to this impairment: no study focused on the role of gender-based effects of attachment styles and contamination symptoms. The Dual Control Model assumes three processes driving sexual arousal: sexual excitation (SE), sexual inhibition (SI) due to threat of performance failure, and SI due to threat of performance consequences (e.g., getting contaminated with sexually transmitted diseases). In a group of OCD patients, we hypothesized that (a) women report lower SE and higher SI than men; (b) patients with insecure (both anxious and avoidant) attachment styles show lower SE and higher SI; (c) attachment styles moderate the relation between gender and sexual arousal (respectively, for women, higher attachment anxiety, and for men higher attachment avoidance were related to impaired sexual arousal (higher SE and SI) controlling for OCD severity); and (d) contamination symptoms moderate the relation between gender and sexual impairment (women with contamination symptoms show impaired sexual arousal). Seventy-two OCD patients (37.50% women) completed the Obsessive–Compulsive Inventory-Revised, Attachment Styles Questionnaire and Sexual Inhibition/Sexual Excitation Scales. In contrast with our hypotheses, women reported higher SE and lower SI due to threat of performance consequences than men. Patients with higher attachment avoidance (discomfort with intimacy) but also confidence in self and others had higher SE. Women with attachment avoidance (i.e., discomfort with intimacy) had lower SE, while women with attachment anxiety (i.e., preoccupations with relationships) had higher SI due to negative performance consequences. Women with contamination symptoms had higher SI due to performance failure but lower SI due to performance consequences. The present preliminary findings suggest that sexual arousal impairment should be evaluated during the assessment of OCD patients, and gender-based effects of attachment styles and contamination symptoms should be considered during personalized treatment planning.
Impact of SARS-CoV-2 Infection on Cognitive Function: A Systematic Review
Authors: Alnefeesi Yazen , Siegel Ashley , Lui Leanna M. W. , Teopiz Kayla M. , Ho Roger C. M. , Lee Yena , Nasri Flora , Gill Hartej , Lin Kangguang , Cao Bing , Rosenblat Joshua D. , McIntyre Roger S.
Published: February 2021

The prevalence and etiology of COVID-19's impact on brain health and cognitive function is poorly characterized. With mounting reports of delirium, systemic inflammation, and evidence of neurotropism, a statement on cognitive impairment among COVID-19 cases is needed. A substantial literature has demonstrated that inflammation can severely disrupt brain function, suggesting an immune response, a cytokine storm, as a possible cause of neurocognitive impairments. In this light, the aim of the present study was to summarize the available knowledge of the impact of COVID-19 on cognition (i.e., herein, we broadly define cognition reflecting the reporting on this topic in the literature) during the acute and recovery phases of the disease, in hospitalized patients and outpatients with confirmed COVID-19 status. A systematic review of the literature identified six studies which document the prevalence of cognitive impairment, and one which quantifies deficits after recovery. Pooling the samples of the included studies (total sample n = 644) at three standards of quality produced conservative estimates of cognitive impairment ranging from 43.0 to 66.8% prevalence in hospitalized COVID-19 patients only, as no studies which report on outpatients met criteria for inclusion in the main synthesis. The most common impairment reported was delirium and frequent reports of elevated inflammatory markers suggest etiology. Other studies have demonstrated that the disease involves marked increases in IL-6, TNFα, and IL-1β; cytokines known to have a profound impact on working memory and attention. Impairment of these cognitive functions is a characteristic aspect of delirium, which suggests these cytokines as key mediators in the etiology of COVID-19 induced cognitive impairments. Researchers are encouraged to assay inflammatory markers to determine the potential role of inflammation in mediating the disturbance of cognitive function in individuals affected by COVID-19.
Deciphering the Link Between Mental Health and Green Space in Shenzhen, China: The Mediating Impact of Residents' Satisfaction
Authors: Qiao Yuhan , Chen Zini , Chen Yuqing , Zheng Tianxiang
Published: February 2021

Rapid urbanization and increasing urban density in China threaten residents' mental health. As a vital component of built environments, green space plays a key role in individuals' psychological well-being; however, the mediating effect of residents' satisfaction with the green space environment on the relationship between urban greening and residents' mental health in Chinese contexts has yet to be thoroughly explored. To fill this knowledge gap, this paper attempts to reveal the internal logic and mechanism underlying the linkages between green space, residents' mental health, and their satisfaction with green space in Shenzhen, China. Specifically, this paper explores the mediating role of residents' satisfaction with a green space environment using questionnaire survey data, “Quick Bird-2” high-resolution remote sensing image data, and a multilevel regression model. Our empirical findings indicate that the relative range of neighboring green spaces can directly improve residents' mental health. More importantly, the relationship between the relative scope of green space and residents' mental health is mediated by residents' satisfaction with the green space environment rather than its direct health effects. Given the influence of green space on residents' satisfaction with the environment, green space indirectly affects mental health. These findings should provide the government useful guidance for considering the spatial distribution and quantity of green space. Our results should also help residents improve their actual experiences and subjective satisfaction with the green space environment.
Network Analysis to Identify Communities Among Multiple Exposure Biomarkers Measured at Birth in Three Flemish General Population Samples
Authors: Ottenbros Ilse , Govarts Eva , Lebret Erik , Vermeulen Roel , Schoeters Greet , Vlaanderen Jelle
Published: February 2021

Introduction:Humans are exposed to multiple environmental chemicals via different sources resulting in complex real-life exposure patterns. Insight into these patterns is important for applications such as linkage to health effects and (mixture) risk assessment. By providing internal exposure levels of (metabolites of) chemicals, biomonitoring studies can provide snapshots of exposure patterns and factors that drive them. Presentation of biomonitoring data in networks facilitates the detection of such exposure patterns and allows for the systematic comparison of observed exposure patterns between datasets and strata within datasets.

Methods:We demonstrate the use of network techniques in human biomonitoring data from cord blood samples collected in three campaigns of the Flemish Environment and Health Studies (FLEHS) (sampling years resp. 2002–2004, 2008–2009, and 2013–2014). Measured biomarkers were multiple organochlorine compounds, PFAS and metals. Comparative network analysis (CNA) was conducted to systematically compare networks between sampling campaigns, smoking status during pregnancy, and maternal pre-pregnancy BMI.

Results:Network techniques offered an intuitive approach to visualize complex correlation structures within human biomonitoring data. The identification of groups of highly connected biomarkers, “communities,” within these networks highlighted which biomarkers should be considered collectively in the analysis and interpretation of epidemiological studies or in the design of toxicological mixture studies. Network analyses demonstrated in our example to which extent biomarker networks and its communities changed across the sampling campaigns, smoking status during pregnancy, and maternal pre-pregnancy BMI.

Conclusion:Network analysis is a data-driven and intuitive screening method when dealing with multiple exposure biomarkers, which can easily be upscaled to high dimensional HBM datasets, and can inform mixture risk assessment approaches.
PTSD and Suicidal Behaviors Amongst L'Aquila 2009 Earthquake Young Survivors
Authors: Carmassi Claudia , Bertelloni Carlo Antonio , Dell'Oste Valerio , Luperini Chiara , Marazziti Donatella , Rossi Rodolfo , Dell'Osso Liliana
Published: February 2021

Background: Post-traumatic stress disorder (PTSD) is one of the most frequent and severe psychiatric consequences of natural disasters, frequently associated with suicidality. The aim of this study was at examining the possible relationships between suicidal behaviors and full-blown or partial PTSD, in a sample of young earthquake survivors. The second aim was at investigating the specific role of PTSD symptoms on suicidality.

Methods: A total of 475 young adults who survived the L'Aquila 2009 earthquake, one of the most severe Italian disasters of the last decades, were recruited and assessed after 21 months from the catastrophe. Participants were evaluated by two questionnaires assessing subthreshold psychopathology, the Trauma and Loss Spectrum Self-Report (TALS-SR) to investigate both full and partial PTSD, and two specific Mood Spectrum Self-Report (MOODS-SR) sub-domains exploring suicidality, namely suicidal ideation and suicide attempts.

Results: The ensuing findings showed that suicidal ideation and suicide attempts were present, respectively, in 40 (8.4%) and 11 (2.3%) survivors. Rates of suicidal ideation were significantly more elevated in full-blown PTSD subjects (group 1), as compared with those suffering from partial (group 2) or no PTSD (group 3). Interestingly, group 2 subjects showed significantly more suicidal ideation than healthy individuals, and less than those of group 1, while the frequency of suicide attempts was similar across the three groups. Suicidal ideation was associated with higher scores in the following TALS-SR domains: grief-reactions, re-experiencing, avoidance and numbing, maladaptive coping, and personal characteristics/risk factor.

Conclusions: The results of the present study support and extend previous findings on the role of PTSD symptoms in suicidality after a severe earthquake. However, as compared with available literature, they also highlight the significant impact of sub-threshold PTSD manifestations in increasing the suicide risk in survivors of a mass disaster.
Our Collective Needs and Strengths: Urban AI/ANs and the COVID-19 Pandemic
Authors: Maudrie Tara L. , Lessard Kerry Hawk , Dickerson Jessica , Aulandez Kevalin M. W. , Barlow Allison , O’Keefe Victoria M.
Published: February 2021

The COVID-19 pandemic has raised national consciousness about health inequities that disproportionately impact American Indian/Alaska Native (AI/AN) communities, yet urban AI/AN communities continue to remain a blind spot for health leaders and policymakers. While all United States cities have been the traditional homelands of AI/AN peoples since time immemorial, urban AI/ANs are consistently excluded in local and national health assessments, including recent reports pertaining to COVID-19. Today the majority of AI/ANs (71%) live in urban areas, and many cities have strong Urban Indian Health Programs (UIHPs) that provide space for medical care, community gatherings, cultural activities, and traditional healing. Many of these UIHPs are currently scrambling to meet the needs of their AI/AN service communities during the pandemic. While the COVID-19 pandemic brought new sources of funding to UIHPs, the lack of local AI/AN data and arbitrary funding restrictions precluded some UIHPs from addressing their communities’ most immediate challenges such as food and economic insecurities. Despite these challenges, urban AI/AN communities carry the historical resilience of their ancestors as they weave strong community networks, establish contemporary traditions, and innovate to meet community needs. This article focuses on the experiences of one UIHP in Baltimore City during the COVID-19 pandemic to illustrate present-day challenges and strengths, as well as illuminate the urgency for tailored, local data-driven public health approaches to urban AI/AN health.
The Nairobi Pork Value Chain: Mapping and Assessment of Governance, Challenges, and Food Safety Issues
Authors: Murungi Maurice K. , Muloi Dishon M. , Muinde Patrick , Githigia Samuel Maina , Akoko James , Fèvre Eric M. , Rushton Jonathan , Alarcon Pablo
Published: February 2021

The Nairobi pork food system is a growing livestock sub-sector which serves as a source of food and livelihood to its inhabitants. The study aimed to map Nairobi's pork value chains, assess their governance, operational challenges and their impacts on food safety risks and management practices. Qualitative data were collected in seven focus group discussions and 10 key informants' interviews on animal movements and product flows, stakeholders' interactions, perceptions on system governance and challenges, and on their potential impact on food safety management. Quantitative data were obtained to show the importance of flows, business operations and market share. Thematic analysis was conducted to identify themes that provide understanding on the governance, challenges and food safety practices in each profile. The predominant chains identified were [1] The “large integrated company” profile which accounted for 83.6% of pork marketed through abattoirs, and was based on a well-structured supply system, with owned farms (representing 50% of their supply), contract farms and semi-contract farms and [2] Local independent abattoirs, accounting for 16.4%, are privately owned small-scale production, supplied mainly (70%) by small farmers from the immediate neighboring areas. The main challenges associated with governance themes included; (i) Inadequate/lack of enforcement of existing regulation (ii) Negative effect of devolution system of governance (iii) Pig traders' dominance (iii) Lack of association at all system nodes, and (iv) Male dominance across the pig system. The main challenges reported included; (i) Lack of capital to upscale (ii) Poor infrastructure (iii) Pig shortage (iv) Excessive regulation (v) Lack of training (vi) Diseases (v) Lack of knowledge (vi) Unfair competition. Food safety themes were associated with (i) Inadequate slaughter facilities forcing traders/farmers to undertake home slaughter (ii) Lack of knowledge on disease management (iii) Lack of training on hygienic practices in the slaughterhouse and (iv) Lack/insufficient capital to purchase equipment's to ensure proper hygiene e.g., boilers. The study provides insights into the structure of the pork system supplying Nairobi, the governance issues important to the stakeholders, challenges and food safety issues. The framework obtained can be used by policy makers and researchers to investigate and develop pork industry and for food safety and disease control programmes.
An Iterative, Frequentist Approach for Latent Class Analysis to Evaluate Conditionally Dependent Diagnostic Tests
Authors: Schoneberg Clara , Kreienbrock Lothar , Campe Amely
Published: February 2021

Latent class analysis is a well-established method in human and veterinary medicine for evaluating the accuracy of diagnostic tests without a gold standard. An important assumption of this procedure is the conditional independence of the tests. If tests with the same biological principle are used, this assumption is no longer met. Therefore, the model has to be adapted so that the dependencies between the tests can be considered. Our approach extends the traditional latent class model with a term for the conditional dependency of the tests. This extension increases the number of parameters to be estimated and leads to negative degrees of freedom of the model, meaning that not enough information is contained in the existing data to obtain a unique estimate. As a result, there is no clear solution. Hence, an iterative algorithm was developed to keep the number of parameters to be estimated small. Given adequate starting values, our approach first estimates the conditional dependencies and then regards the resulting values as fixed to recalculate the test accuracies and the prevalence with the same method used for independent tests. Subsequently, the new values of the test accuracy and prevalence are used to recalculate the terms for the conditional dependencies. These two steps are repeated until the model converges. We simulated five application scenarios based on diagnostic tests used in veterinary medicine. The results suggest that our method and the Bayesian approach produce similar precise results. However, while the presented approach is able to calculate more accurate results than the Bayesian approach if the test accuracies are initially misjudged, the estimates of the Bayesian method are more precise when incorrect dependencies are assumed. This finding shows that our approach is a useful addition to the existing Bayesian methods, while it has the advantage of allowing simpler and more objective estimations.
Serum and Tissue Expression Levels of Leptin and Leptin Receptor Are Putative Markers of Specific Feline Mammary Carcinoma Subtypes
Authors: Gameiro Andreia , Nascimento Catarina , Urbano Ana Catarina , Correia Jorge , Ferreira Fernando
Published: February 2021

Obesity is an established risk factor for breast cancer in post-menopausal women, being associated with elevated serum levels of leptin. Although overweight is a common condition in cat, the role of leptin and its receptor in feline mammary carcinoma remains unsettled. In this study, serum leptin and leptin receptor (ObR) levels were investigated in 58 cats with mammary carcinoma and compared with those of healthy animals, as were the expression levels of leptin and ObR in tumor tissues. The results showed that the Free Leptin Index is significantly decreased in cats with mammary carcinoma (p= 0.0006), particularly in those with luminal B and HER2-positive tumors, and that these animals also present significantly lower serum leptin levels (p< 0.0001 andp< 0.005, respectively). Interestingly, ulcerating tumors (p= 0.0005) and shorter disease-free survival (p= 0.0217) were associated to serum leptin levels above 4.17 pg/mL. In contrast, elevated serum ObR levels were found in all cats with mammary carcinoma (p< 0.0001), with levels above 16.89 ng/mL being associated with smaller tumors (p= 0.0118), estrogen receptor negative status (p= 0.0291) and increased serum levels of CTLA-4 (p= 0.0056), TNF-α (p= 0.0025), PD-1 (p= 0.0023), and PD-L1 (p= 0.0002). In tumor samples, leptin is overexpressed in luminal B and triple-negative carcinomas (p= 0.0046), whereas ObR is found to be overexpressed in luminal B tumors (p= 0.0425). Altogether, our results support the hypothesis that serum levels of leptin and ObR can be used as biomarkers of specific feline mammary carcinoma subtypes, and suggests the use of leptin antagonists as a therapeutic tool, reinforcing the utility of the cat as a cancer model.
Preliminary Effects of Crop Residue Management on Soil Quality and Crop Production under Different Soil Management Regimes in Corn-Wheat Rotation Systems
Authors: Mirzaei Morad , Gorji Anari Manouchehr , Razavy-Toosi Ehsan , Asadi Hossein , Moghiseh Ebrahim , Saronjic Nermina , Rodrigo-Comino Jesús
Published: February 2021

Strategic management of crop residues is essential to enhance soil quality for sustainable agriculture. However, little is known about the specific amounts of crop residues needed to improve soil quality characteristics which are key to develop economic plans. In this study, we investigated the effects of applying crop residue at five rates, including 100% (R100), 75% (R75), 50% (R50), 25% (R25), and 0% (R0), on wheat yield and soil properties. Field experiments were conducted for two cropping seasons in a wheat-corn rotation under conventional (CT) and no-till (NT) systems to observe the first results obtained during short-term periods (one-year application). During the study, the wheat and corn fields were irrigated. Application of plant residue resulted in increased soil organic carbon (SOC) and available nutrients and improved soil physical properties, i.e., aggregates mean weight diameter in wet (MWDw) and dry (MWDd) conditions, water-stable aggregates (WSA), dry-stable aggregates, (DSA), soil water infiltration (SWI), soil available water (SAW), and yield of wheat and corn. The effects were stronger at higher residue application rates. In the CT system, compared to R0, R100 resulted in the highest increase equal to 38, 29, 23, 34, 35, 41, and 11% for SOC, MWDw, MWDd, WSA, DSA, SAW, and wheat grain yield, respectively. This was equivalent to 28, 19.5, 19, 37, 44, 52, and 6% for the NT system, respectively. Generally, the NT system resulted in a stratification of the soil properties within 0–10 cm compared to 10–20 cm soil depth, but a uniform distribution for both depths under CT system. Overall, these results show that crop residue application can improve soil quality and yield in cereal production systems under semi-arid conditions during the first year of application. It will be key to monitor these changes in along-term field studies.