bluuebunny/crossref_metadata_2025 huggingface dataset:
Dirofilaria immitis Could Be a Risk Factor for the Development of Allergic Diseases in Humans
Authors: Montoya-Alonso José Alberto , Morchón Rodrigo , Matos Jorge Isidoro , Falcón-Cordón Yaiza , Costa-Rodriguez Noelia , Carretón Elena
Published: October 2020

The Canary Islands are hyperendemic for canine heartworm and the risk of zoonotic infection is high. Additionally, there is evidence of development of allergies due to nematode infections in animals and humans. Since the population of the Canary Islands presents high prevalence of allergic comorbidities, and previous studies have shown a possible relationship between allergies and seropositivity to heartworm, the aim was to evaluate whether exposure to heartworm may contribute to the development of allergies in the human population. First, an epidemiological study was conducted on 248 owners of dogs with/without heartworm infection in the Canary Islands. Secondly, a serological analysis of the presence of total IgE and specific IgE against heartworm was conducted in 66 samples of inhabitants of the Canary Islands. The survey showed that allergic owners had heartworm-positive dogs more frequently (p < 0.01). Of the analyzed human samples, 43.9% were seropositive to heartworm and Wolbachia. Total IgE concentrations were increased in 34.5% seropositive samples and 8.1% seronegative samples. Specific IgE against heartworm was only found in human seropositive samples (17.2%). Contact with heartworm may be a risk factor for the development of allergic diseases, although further studies to elucidate the relationship between heartworm and allergies should be carried out.
Analysis of lncRNA Expression Profile during the Formation of Male Germ Cells in Chickens
Authors: Gao Wen , Zhang Chen , Jin Kai , Zhang Yani , Zuo Qisheng , Li Bichun
Published: October 2020

Germ cells have an irreplaceable role in transmitting genetic information from one generation to the next, and also play an important role in sex differentiation in poultry, while little is known about epigenetic factors that regulate germ cell differentiation. In this study, RNA-seq was used to detect the expression profiles of long non-coding RNAs (lncRNAs) during the differentiation of chicken embryonic stem cells (ESCs) into spermatogonial stem cells (SSCs). The results showed that a total of 296, 280 and 357 differentially expressed lncRNAs (DELs) were screened in ESCs vs. PGCs, ESCs vs. SSCs and PGCs vs. SSCs, respectively. Gene Ontology (GO) and KEGG enrichment analysis showed that DELs in the three cell groups were mainly enriched in autophagy, Wnt/β-catenin, TGF-β, Notch and ErbB and signaling pathways. The co-expression network of 37 candidate DELs and their target genes enriched in the biological function of germ cell development showed that XLOC_612026, XLOC_612029, XLOC_240662, XLOC_362463, XLOC_023952, XLOC_674549, XLOC_160716, ALDBGALG0000001810, ALDBGALG0000002986, XLOC_657380674549, XLOC_022100 and XLOC_657380 were the key lncRNAs in the process of male germ cell formation and, moreover, the function of these DELs may be related to the interaction of their target genes. Our findings preliminarily excavated the key lncRNAs and signaling pathways in the process of male chicken germ cell formation, which could be helpful to construct the gene regulatory network of germ cell development, and also provide new ideas for further optimizing the induction efficiency of germ cells in vitro.
Low-Protein Diet Supplemented with Medium-Chain Fatty Acid Glycerides Improves the Growth Performance and Intestinal Function in Post-Weaning Piglets
Authors: Cui Zhijuan , Wang Xianze , Hou Zhenping , Liao Simeng , Qi Ming , Zha Andong , Yang Zhe , Zuo Gang , Liao Peng , Chen Yuguang , Tan Bie
Published: October 2020

Medium-chain fatty acid glycerides have been shown to provide energy for rapid oxidation in the body. The study was conducted to investigate the effects of dietary supplementation with medium-chain fatty acid glyceride on the growth performance and intestinal health of weaned piglets fed with a low-protein diet. Nighty healthy weaned piglets were randomly divided into five treatments: NP (Normal protein treatment, normal-protein diet no antibiotics included); NC (Negative control, low-protein diet no antibiotics included); PC (Positive control, low-protein diet +75 mg/kg quinocetone, 20 mg/kg virginiamycin and 50 mg/kg aureomycin); MCT (tricaprylin + tricaprin treatment, low-protein diet + tricaprylin + tricaprin); GML (glycerol monolaurate treatment, low-protein diet + glycerol monolaurate). The results showed that the average daily feed intake (ADFI) of the MCT treatment was significantly higher than that of the NP, NC treatments (p < 0.05). In the jejunum, the villus height of the GML treatment was significantly lower than that of the PC treatment (p < 0.05), and the number of goblet cells in the GML treatment was higher than that in the NC treatment (p < 0.05). Compared with the NC treatment, the MCT treatment significantly increased the level of claudin-1, Zonula occludens-1(ZO-1), while the GML treatment significantly increased the level of claudin-1, occludin, ZO-1 (p < 0.05). In the ileum, the level of ZO-1 in the GML treatment was significantly higher than that in the NP, NC, PC treatments (p < 0.05). Compared with the NC treatment, the GML treatment significantly increased the level of Secretory immunoglobulin A (SIgA) in the ileum and serum, while the MCT treatment significantly increased the level of SIgA and decreased the level of interleukin-6 (IL-6) in the ileum (p < 0.05). These results showed that the addition of medium-chain fatty acid glycerides to a low-protein diet could improve the growth performance and intestinal functional barrier of weaned piglets and also improve the immune function of weaned piglets.
Effect of Parity on Reproductive Performance and Composition of Sow Colostrum during First 24 h Postpartum
Authors: Segura Mónica , Martínez-Miró Silvia , López Miguel José , Madrid Josefa , Hernández Fuensanta
Published: October 2020

The aim of this study is to assess the effects of parity number on sow reproductive performance and the chemical and immunological composition of colostrum and immunoglobin concentrations in the sera of the sows. Colostrum samples were collected at 0, 6 and 24 h after the births of the first piglets from 56 sows with different numbers of parturitions (ranging 1–6). The piglets born alive to primiparous sows had lower birth weights (p < 0.05) than piglets from second and fourth parturition sows. The colostrum composition was influenced (p < 0.05) by parity number: primiparous sows had higher concentrations of dry matter, fat, lactose and non-fat-solids. No parity-dependent differences were found concerning total protein amount. Colostrum composition was drastically affected (p < 0.001) by sampling time—the highest concentrations of dry matter and protein and lowest concentrations of fat and lactose were found immediately after parturition (0 h). The study revealed no effect of parity (p ≥ 0.05) on the concentrations of immunoglobulins in colostrum. The immunoglobulin with the highest level in sow serum at day 110 of gestation was IgG, while IgA showed the lowest values and greater variability with respect to parity from an immunological point of view. Regarding the relationship between serum Ig levels at the end of gestation and colostrum Ig, serum IgG showed a strong correlation with colostrum IgG and IgM, while colostrum IgG was strongly related with colostrum IgM, but not with IgA. IgA did not correlate with any other immunoglobulin. The different behaviors of the immunoglobins in colostrum were probably due to IgG coming almost exclusively from the sows’ sera, whereas IgA is mainly synthetized by the mammary gland.
The Impact of Ammoniation Treatment on the Chemical Composition and In Vitro Digestibility of Rice Straw in Chinese Holsteins
Authors: Ma Yulin , Chen Xu , Zahoor Khan Muhammad , Xiao Jianxin , Liu Shuai , Wang Jingjun , He Zhiyuan , Li Congcong , Cao Zhijun
Published: October 2020

The current study was conducted to explore the ammoniation treatment effects on the chemical composition and in vitro digestibility of rice straw in Chinese Holsteins. For this purpose, rice straw was stored in polyethylene bags (35 × 25 cm, 350 g per bag) including (i) no additives (RS); (ii) 5% urea (5U, dry matter (DM) basis); (iii) 9% corn steep liquor + 5% urea (9C5U, DM basis); (iv) 9C2.5U; and (v) 9C2.5U + 3% molasses (9C2.5U3M, DM basis). The air-dry matter of the mixture was kept at the same level at 55% for all treatments. Fifteen bags (5 treatments × 3 repeats) were prepared and stored at ambient temperature (25 ± 3 °C). The chemical composition and in vitro digestibility were measured at day 60 after storage. Our analysis revealed that all the four ammoniation treatments improved the in vitro DM and neutral detergent fiber (IVNDFD) digestibility. In addition, all the four ammoniation treatments significantly (P < 0.001) increased the levels of crude protein (CP), gas production (GP), acetic acid (AA), butyric acid (BA) and total volatile fatty acid (TVFA) contents of the rice straw and decreased the neutral detergent fiber (NDF) and acid detergent fiber (ADF) of the rice straw compared to the control. Within four treated groups, the 9C5U treatment was most effective. Finally, we concluded that ammoniation treatments increased the nutritive value of rice straw. In addition the 9C5U treatment could be an effective ammoniation treatment for the better utilization of rice straw.
Effect of Physical Form and Level of Wheat Straw Inclusion on Growth Performance and Blood Metabolites of Fattening Goat
Authors: Malik Muhammad I. , Rashid Muhammad A. , Yousaf Muhammad S. , Naveed Saima , Javed Khalid , Rehman Habib
Published: October 2020

The inclusion of straw in high concentrate total mixed rations (TMRs) of male fattening goats can provide the necessary fiber to prevent ruminal acidosis and maintain growth. The objective of this study was to evaluate the effects of the physical form (PF) of the diet (pelleted vs. conventional) and the straw level (SL) of wheat straw (WS) (15% versus 25%) in total mixed rations on feed intake, growth, total tract digestibility, and blood metabolites of fattening goats. Thirty-two male Beetal goats (27.4 ± 0.28 kg body weight (BW)) were divided randomly into the following four dietary treatments with a 2 × 2 factorial arrangement (n = 8/treatment): (1) CTMR15 (conventional TMR containing 15% WS), (2) CTMR25 (conventional TMR containing 25% WS), (3) PTMR15 (pelleted TMR containing 15% WS), and (4) PTMR25 (pelleted TMR containing 25% WS). Both conventional and pelleted 15% WS TMR had 33.7% neutral detergent fiber (NDF) and 19.3% acid detergent fiber (ADF), whereas in 25% WS TMR the NDF and ADF contents were 38.7% and 22.9%, respectively. The experimental diets were formulated to be iso-nitrogenous (crude protein (CP) = 15%). The dry matter intake (DMI) (1.265 vs. 1.044 kg/day) and average daily gain (ADG) (0.176 vs. 0.143 kg/day) were higher (p < 0.05) in pelleted vs. conventional TMR-fed goats. Irrespective of the PF of the TMR, the 15% WS-fed animals had greater (p < 0.05) DMI (1.206 vs. 1.102 kg/day) and ADG (0.172 vs. 0.144 kg) when compared to those fed on 25% WS diets. Furthermore, feed-to-gain ratio (F:G) was higher (p < 0.05) in the 25% WS-fed goats when compared with the 15% WS-fed animals. Digestibility coefficients, nitrogen balancing, hepatic enzymes, blood metabolites, and hematological parameters were similar (p > 0.05) across all treatments. In conclusion, feeding pelleted TMR with WS improved DMI and growth performance as compared to those fed conventional TMR, and 15% WS performed better than 25% WS without exerting any adverse effects on blood metabolites, liver enzymes, or hematological parameters.
Varying Opinions about Animal Welfare in the Australian Live Export Industry: A Survey
Authors: Fleming Patricia A. , Wickham Sarah L. , Barnes Anne L. , Miller David W. , Collins Teresa
Published: October 2020

There is significant public interest in the Australian live animal export industry and a need to develop a program that can measure and monitor animal welfare throughout the supply chain. An online survey of stakeholder opinions of this industry and animal welfare measures was carried out in 2015 with 921 respondents: 30% from the public who identified as animal welfare advocates (AWAs); 44% from the public who did not identify as AWAs (general public; GP); 26% live export industry (LEI) workers. AWA and GP respondents expressed greater concern than LEI respondents for animal welfare throughout the supply chain but had less concern for animals at Australian feedlots than in other parts of the supply chain. The majority of AWA and GP respondents believed data collected on animal welfare should be made public and should be collected by independent welfare officers and used to regulate the industry and impose penalties for poor welfare. LEI workers believed that data should be confidential, collected by LEI workers and used by the industry to self-regulate. AWA and GP respondents rated the importance and practicality of a number of welfare indicators greater than LEI workers, while respondents shared an analogous view of the importance and practicality of these indicators. Results can be used to develop welfare assessments that ensure a better understanding between industry members and those not in the industry, while facilitating welfare improvements and promoting greater transparency for the live export industry.
Multifactorial Causes of Chronic Mortality in Juvenile Sturgeon (Huso huso)
Authors: Ciulli Sara , Volpe Enrico , Sirri Rubina , Tura Giorgia , Errani Francesca , Zamperin Gianpiero , Toffan Anna , Silvi Marina , Renzi Andrea , Abbadi Miriam , Biasini Lorena , Pretto Tobia , Emmanuele Pietro , Casalini Antonio , Sarli Giuseppe , Serratore Patrizia , Mordenti Oliviero , Mandrioli Luciana
Published: October 2020

This investigation focused on an episode of chronic mortality observed in juvenile Huso huso sturgeons. The examined subjects underwent pathological, microbiological, molecular, and chemical investigations. Grossly severe body shape deformities, epaxial muscle softening, and multifocal ulcerative dermatitis were the main observed findings. The more constant histopathologic findings were moderate to severe rarefaction and disorganization of the lymphohematopoietic lymphoid tissues, myofiber degeneration, atrophy and interstitial edema of skeletal epaxial muscles, and degeneration and atrophy of the gangliar neurons close to the myofibers. Chemical investigations showed a lower selenium concentration in affected animals, suggesting nutritional myopathy. Other manifestations were nephrocalcinosis and splenic vessel wall hyalinosis. Septicemia due to bacteria such as Aeromonas veronii, Shewanella putrefaciens, Citrobacter freundii, Chryseobacterium sp., and pigmented hyphae were found. No major sturgeon viral pathogens were detected by classical methods. Next-generation sequencing (NGS) analysis confirmed the absence of viral pathogens, with the exception of herpesvirus, at the order level; also, the presence of Aeromonas veronii and Shewanella putrefaciens was confirmed at the family level by the metagenomic classification of NGS data. In the absence of a primary yet undetected biological cause, it is supposed that environmental stressors, including nutritional imbalances, may have led to immune system impairment, facilitating the entry of opportunistic bacteria and mycotic hyphae.
A Multilevel Intervention Framework for Supporting People Experiencing Homelessness with Pets
Authors: Kerman Nick , Lem Michelle , Witte Mike , Kim Christine , Rhoades Harmony
Published: October 2020

Approximately one in 10 people experiencing homelessness have pets. Despite the psychosocial benefits derived from pet ownership, systemic and structural barriers can prevent this group from meeting their basic needs and exiting homelessness. A multilevel framework is proposed for improving the health and well-being of pet owners experiencing homelessness. Informed by a One Health approach, the framework identifies interventions at the policy, public, and direct service delivery levels. Policy interventions are proposed to increase the supply of pet-friendly emergency shelters, access to market rental housing and veterinary medicine, and the use of a Housing First approach. At the public level, educational interventions are needed to improve knowledge and reduce stigma about the relationship between homelessness and pet ownership. Direct service providers can support pet owners experiencing homelessness by recognizing their strengths, connecting them to community services, being aware of the risks associated with pet loss, providing harm reduction strategies, documenting animals as emotional support animals, and engaging in advocacy. By targeting policies and service approaches that exacerbate the hardships faced by pet owners experiencing homelessness, the framework is a set of deliberate actions to better support a group that is often overlooked or unaccommodated in efforts to end homelessness.
cAMP Response Element Binding Protein 1 (CREB1) Promotes Monounsaturated Fatty Acid Synthesis and Triacylglycerol Accumulation in Goat Mammary Epithelial Cells
Authors: Yao Dawei , Yang Chunlei , Ma Jing , Chen Lili , Luo Jun , Ma Yi , Loor Juan. J.
Published: October 2020

cAMP response element binding protein 1 (CREB1) is a member of the leucine zipper transcription factor family of DNA binding proteins. Although studies in non-ruminants have demonstrated a crucial role of CREB1 in lipid synthesis in liver and adipose tissue, it is unknown if this transcription regulator exerts control of fatty acid synthesis in ruminant mammary cells. To address this question, we first defined the expression dynamics of CREB1 in mammary tissue during lactation. Analysis of CREB1 in mammary tissue revealed higher mRNA abundance in mammary tissue harvested at peak lactation. Overexpression of CREB1 markedly upregulated sterol regulatory element binding transcription factor 1 (SREBP1), fatty acid synthase (FASN), acetyl-coenzyme A carboxylase α (ACACA), elongase of very long chain fatty acids 6 (ELOVL6), lipoprotein lipase (LPL), fatty acid binding protein 3 (FABP3), lipin 1 (LPIN1) and diacylglycerol acyltransferase 1 (DGAT1), but had no effect on glycerol-3-phosphate acyltransferase, mitochondrial (GPAM) or 1-acylglycerol-3-phosphate O-acyltransferase 6 (AGPAT6). In addition, overexpressing CREB1 led to a significant increase in the concentration and desaturation index of C16:1 (palmitoleic acid) and C18:1 (oleic acid), along with increased concentration of triacylglycerol. Taken together, these results highlight an important role of CREB1 in regulating lipid synthesis in goat mammary epithelial cells. Thus, manipulation of CREB1 in vivo might be one approach to improve the quality of goat milk.
Bioaccumulation of Mineral Elements in Different Biological Substrates of Athletic Horse from Messina, Italy
Authors: Fazio Francesco , Gugliandolo Enrico , Nava Vincenzo , Piccione Giuseppe , Giannetto Claudia , Licata Patrizia
Published: October 2020

The objective of this study was to evaluate the levels and the potential bioaccumulation of vanadium (V), chromium (Cr), cobalt (Co), copper (Cu), zinc (Zn), cadmium (Cd), lead (Pb), and bismuth (Bi) in horses from the industrial risk area of Sicily (Italy). Different biological substrates (whole blood, serum; tail and mane) and samples of hay, concentrate and water provided to the horses were processed by means of Thermo Scientific iCAP-Q ICP–MS spectrometer for mineral concentration. One-way analysis of variance (ANOVA) was applied to show the differences in various trace elements in the biological substrates. Pearson’s test was applied to evaluate the correlation of mineral concentrations between whole blood and serum; and tail and mane. The results showed statistical differences of tested mineral elements among biological substrates; Cr whole blood concentrations were negatively correlated with serum concentrations and a positive correlation between whole blood and serum was observed for Cd and Bi. This latter also showed a positive correlation between mane and tail. The concentrations of V, Cr, and Pb in tail with serum and whole blood samples were negatively correlated, while the concentrations of Cd in tail and serum samples were positively correlated. Minerals had a non-homogenous distribution in the organism, showing different concentrations in the biological substrates.
Effect of Pre-Blended Phosphates on the Freezing Quality Characteristics of Ground Woody Breast Meat Compared to Normal Meat
Authors: Garner Laura J. , Brooks Lasheda , Spencer Lindsey F. , Rehm John , Kataria Jasmine , Morey Amit
Published: October 2020

Woody breast (WB) myopathy affected meat has a tough texture, higher cook loss, and decreased water holding capacity (WHC), and thus lower consumer acceptability. The WB meat can be ground and further converted into further processed products or frozen, stored, and shipped to further processors. Freezing and thawing of ground WB meat may further affect the quality of WB meat products. Hence, research is required to determine the effect of pre-blended phosphates on the quality of ground WB meat as well as its cryoprotective effect during frozen storage. The objective of this experiment was to investigate the effect of pre-blended phosphate levels on meat quality in WB and normal breast (NB) fillets before and after freezing. NB fillets and severely affected WB fillets were procured from a local commercial processor. The meat was separated into various treatment groups according to the sodium tripolyphosphate (STPP) inclusion levels (0, 0.25, and 0.5% w/w). The meat was ground with respective phosphate treatments and subdivided into vacuum-sealed bags (n = 240; 1 kg each). Half of the bags (n = 120) from each treatment were taken for meat quality analysis, while the other bags were placed in a freezer (−18 °C) for 6 days. Fresh samples were analyzed within 6–8 h while the frozen samples were thawed for 18 h at 4°C prior to analysis. Samples (n = 10) were analyzed for gel strength, pH, color (L* a* b*), proximate composition, and randomly selected samples (n = 5) were analyzed for aerobic plate count (APC). Experiments were repeated in two separate replications and the data was analyzed using the Proc Glimmix model procedure in SAS (v. 9.4) (Cary, NC, USA) with LSMeans Separation at p ≤ 0.05. The gel strength (g) of the fresh ground NB meat (883.7 g) was higher than the gel strength of woody meat (720.8 g) with 0% phosphate (p ≤ 0.05). Addition of phosphate (0.25 and 0.5%) significantly increased the gel strength of fresh woody meat but it was significantly lower than NB meat added with 0.25 and 0.5% phosphate treatment. After freezing, ground NB meat samples with 0.25 and 0.5% phosphate had higher gel strength compared to fresh and frozen ground WB meat (p ≤ 0.05). Pre-blended STPP raised the pH in all treatments (p < 0.05). Treatments did not have any clear impact on APC of ground WB or NB meat. Addition of pre-blended sodium tripolyphosphate increases the functionality of fresh and frozen ground WB meat, as well as NB meat.
Effect of the Inclusion of Different Levels of Dietary Cactus (Opuntia ficus-indica) on Gilts’ Biochemical Parameters and Feed Intake during Lactation
Authors: Ortiz Ruy , López Manuel , Pérez Rosa E. , Ramírez Paola de la Paz , Ordaz Gerardo
Published: October 2020

The regulation of sows’ metabolic state during the gestation-lactation transition is a requirement for a higher feed intake in lactation, an important aspect in improving animal welfare in current swine production systems. The present study aimed to evaluate the effects of the inclusion of different cactus (Opuntia ficus-indica L.) levels in the diet of gilts during late gestation and lactation on their biochemical parameters and voluntary feed intake during lactation. From day 85 of gestation until weaning, 40 gilts were divided into four groups: GNC (group with no cactus) with a basal diet (BD) only, G1C; group with 1% inclusion of cactus plus BD, G2C; group with 1.5% inclusion of cactus plus BD, and G3C; group with 2% inclusion of cactus plus BD. The dietary cactus supplementation increased the gilts’ feed intake (by 1.04 kg/day on average) during lactation and reduced their weight loss (4.3%) at weaning. The glucose concentrations were higher (range 73.0–83.9 mg/dL) in the GNC. The GNC had the highest triglyceride and cholesterol concentrations at day 100 of gestation. G3C had the highest osteocalcin concentration at day 100 of gestation. The highest feed intake and lowest glucose concentration were achieved with a cactus consumption of 1.04% in lactating gilts.
The Concerns, Difficulties, and Stressors of Caring for Pets during COVID-19: Results from a Large Survey of U.S. Pet Owners
Authors: Applebaum Jennifer W. , Tomlinson Camie A. , Matijczak Angela , McDonald Shelby E. , Zsembik Barbara A.
Published: October 2020

Pets may be a positive presence for their owners during the Coronavirus Disease 2019 (COVID-19) pandemic. However, it is pertinent to identify the hardships associated with pet ownership. We conducted a large-scale survey of U.S. pet owners (n = 2254) in spring and summer 2020 to assess the ways that relationships with pets impacted life during COVID-19. We used thematic analysis to analyze 3671 open-ended responses to three prompts. Reported concerns fell into three major categories: (1) pet-focused (meeting needs of pets; procuring supplies; accessing veterinary care; new and emerging behavioral issues; fate of the pet if owner becomes ill; general safety and well-being), (2) human-focused (issues with working from home; well-being and mental health; balancing responsibilities), and (3) household-focused (disease spread; economic issues). Quantitative analyses showed that the owner’s strength of attachment to their pet, economic resources, and relationship status were associated with the types of concerns expressed. Results from this study indicate that pet owners experienced unique hardships related to changes in everyday life from the COVID-19 pandemic. These hardships should be considered alongside the potential benefits found in other studies in order to manage pet owner expectations, prevent pet relinquishment, and more fully understand multifaceted human-companion animal relationships.
Effect of Breed Types and Castration on Carcass Characteristics of Boer and Large Frame Indigenous Veld Goats of Southern Africa
Authors: van Wyk Gertruida L. , Hoffman Louwrens C. , Strydom Phillip E. , Frylinck Lorinda
Published: October 2020

Weaner male Boer Goats (BG; n = 36; 21 bucks and 15 wethers) and large frame Indigenous Veld Goats (IVG; n = 41; 21 bucks and 20 wethers) were raised on hay and natural grass ad libitum and the recommended amount of commercial pelleted diet to a live weight between 30 and 35 kg. Carcass quality characteristics (live weight, carcass weights, dressing %, chilling loss and eye muscle area) were measured. The right sides of the carcasses were divided into wholesale cuts and dissected into subcutaneous fat, meat and bone. Large frame Indigenous Veld Goat (IVG) wethers were slightly lighter than the IVG bucks with no significant difference observed between BG. Wethers compared to bucks had higher dressing %, subcutaneous fat % in all primal cuts, intramuscular fat %, kidney fat % and, overall, slightly less bone %. Some breed–wether interactions were noticed: IVG wethers were slightly lighter than the IVG bucks, but the IVG bucks tended to produce higher % meat compared to other test groups. Judged on the intramuscular fat % characteristics, it seems as if wethers should produce juicier and more flavorsome meat compared to bucks.
A Comparison of Stocking Methods for Pasture-Based Growing-Finishing Pig Production Systems
Authors: Pietrosemoli Silvana , Green James T. , Villamide Maria Jesús
Published: October 2020

Two alternative stocking methods (rotational and strip-grazing) were compared to continuous stocking at a stocking rate of 47 pigs ha−1 in tall fescue pastures. The research was conducted during two twelve-weeks grazing periods in North Carolina (USA). In total 144 (females and castrated males, 17.5 and 29.1 kg initial body weight) crossbred Yorkshire X Berkshire, Yorkshire/Landrace X Hampshire and Yorkshire/Landrace X Duroc pigs without nose rings were used. Greater soil bulk density and soil concentrations of NO3−, P, K, Mn, Zn and Cu were observed in paddocks managed continuously, while greater final ground cover (+22%) was recorded in paddocks managed with rotational and strip-grazing stocking methods. No differences were detected in botanical composition of the paddocks. Greater weight gains (+8.5%) were registered for rotationally managed pigs. Feed efficiency was better (+8%) for rotationally than for continuously stocked pigs, while strip-grazed pigs presented intermediate values. The results indicated the potential of both alternative stocking methods to be implemented in sustainable pasture-based pig production systems.
Copper Supplementation, A Challenge in Cattle
Authors: López-Alonso Marta , Miranda Marta
Published: October 2020

Ensuring adequate copper supplementation in ruminants is a challenging task due to the complexity of copper metabolism in these animals. The three-way interaction between copper, molybdenum and sulphur (Cu-Mo-S) in the rumen makes ruminants, particularly cattle, very susceptible to suffering from secondary copper deficiency. Paradoxically, excessive copper storage in the liver to prevent deficiency becomes a hazard when ruminants are fed copper-supplemented diets even slightly above requirements. While cattle were traditionally thought to be relatively tolerant of copper accumulation, and reports of copper poisoning were until recently somewhat rare, in recent years an increased number of episodes/outbreaks of copper toxicity in cattle, particularly in dairy cattle, have been reported worldwide. The growing number of lethal cases reported seems to indicate that copper intoxication is spreading silently in dairy herds, urging the development of strategies to monitor herd copper status and improve farmers’ awareness of copper toxicity. In fact, monitoring studies carried out on numerous samples collected from culled animals in slaughterhouses and/or diagnostic laboratories have demonstrated that large numbers of animals have hepatic copper concentrations well above adequate levels in many different countries. These trends are undoubtedly due to copper supplementation aimed at preventing copper deficiency, as dietary copper intake from pasture alone is unlikely to cause such high levels of accumulation in liver tissue. The reasons behind the copper overfeeding in cattle are related both to a poor understanding of copper metabolism and the theory of “if adding a little produces a response, then adding a lot will produce a better response”. Contrary to most trace elements, copper in ruminants has narrow margins of safety, which must also be formulated considering the concentrations of copper antagonists in the diet. This review paper aims to provide nutritionists/veterinary practitioners with the key points about copper metabolism in cattle to guarantee an adequate copper supply while preventing excessive hepatic copper loading, which requires à la carte copper supplementation for each herd.
Does Functionality Condition the Population Structure and Genetic Diversity of Endangered Dog Breeds under Island Territorial Isolation?
Authors: Alanzor Puente José Manuel , Pons Barro Águeda Laura , de la Haba Giraldo Manuel Rafael , Delgado Bermejo Juan Vicente , Navas González Francisco Javier
Published: October 2020

Despite the undefinition of the origins of Ca de Rater (CR) and Ca de Bestiar (CB) dogs, references to these endangered autochthonous breeds highlighted their ratting/pet and shepherding/guard skills for centuries. Genealogical historical records were traced back to founders. Founder number in the reference population (146 and 53 for CR and CB, respectively), historical and reference maximum generations traced (eight and seven for CR and CB, respectively), and historical average number of complete generations (1.04 for both breeds) were determined. Structure assessment revealed the existence of subpopulations regarding criteria such as breeders (75 and 17), breeder location (32 and eight), owners (368 and 198), and owner location (73 and 51) for CR and CB, respectively. Average inbreeding (F) within breed subpopulations ranged from 0.27–1.20% for CB breeders and the rest of subpopulation criteria for both breeds, respectively, except for CB owners and owner location. F ranged from 0.27–1.41% for CB historical population and CR current population, respectively. The study of genetic diversity revealed a relatively similar genetic background between subpopulations. Average coancestry between and within breeds suggested a similar evolutionary process. However, Mann–Whitney U test determined significant differences for diversity parameters (F, ΔR, coancestry, nonrandom mating degree, maximum, complete, and equivalent generations, ΔF, and genetic conservation index) between breeds and their functionalities. Conclusively, functionality in dog breeds may determine the genetic diversity evolution of endangered breeds, even when these share the same geographic isolation conditions.
Intrafollicular and Systemic Dopamine, Noradrenaline and Adrenaline Concentrations in Cycling Mares
Authors: Satué Katiuska , Fazio Esterina , Rubio Maria Dolores , Cravana Cristina , Medica Pietro
Published: October 2020

In some species, catecholamines in follicular fluid (FF) are related to local physiological events responsible for the regulation of ovarian functions and oocyte maturation. The aim of the present study was to determine and compare intrafollicular and systemic concentrations of dopamine (DA), noradrenaline (NA) and adrenaline (AD) in cycling mares. Sixty ovaries were collected during breeding season from 30 mares raised for slaughterhouse meat production, with clinically normal reproductive tracts, were evaluated. Blood samples were collected prior to slaughter. Follicles were classified into three categories in relation to size: small (20–30 mm; n = 20), medium (≥31–40 mm; n = 20) and large (≥41 mm; n = 20). Follicular fluid (FF) samples were extracted from each follicle. Intrafollicular DA, NA and AD concentrations were significantly higher than the systemic concentrations (p < 0.05). Intrafollicular DA concentrations were higher in medium than small and large follicles (p < 0.05). Intrafollicular NA concentrations were higher in small than medium and large follicles (p < 0.05). Intrafollicular AD concentrations were higher in large than small and medium follicles (p < 0.05). Follicle diameter was significantly and negatively correlated with NA and AD (p < 0.05). A significant correlation of the same hormone concentration in FF and in systemic fluid was observed (p < 0.05). In summary, the FF can serve as an intraovarian catecholamine-storing compartment, with the ability to release neurotransmitters in a regulated way. These results provide novel insights into the neuronal nature of the follicle, suggesting the involvement of catecholamines in normal ovarian functions in mares.
Welfare Benefits of Intradermal Vaccination of Piglets
Authors: Temple Déborah , Jiménez Marta , Escribano Damián , Martín-Valls Gerard , Díaz Ivan , Manteca Xavier
Published: October 2020

Vaccination is reported as a stressful and painful event for animals. This study investigated whether needle-free intradermal vaccination improves the welfare of weaned pigs through the reduction of stress and pain biomarkers and improvement of behavioural parameters compared to traditional intramuscular injection with a needle. A total of 339 weaned piglets were allocated to 3 treatment groups: Intradermal Application of Liquids (IDAL) pigs, vaccinated against Porcine Circovirus type 2 (PCV2) by means of intradermal vaccination using a needle-free device Porcilis® PCV ID; Intramuscular (IM) pigs vaccinated against PCV2 with Porcilis® PCV intramuscularly with a needle; CONTROL pigs were managed identically but did not receive any vaccine injection. At the time of the injection, the reaction of IDAL piglets was similar to control piglets, whereas a greater percentage of piglets vaccinated intramuscularly displayed high-pitch vocalizations (7% CONTROL, 7% IDAL, 32% IM) and retreat attempts (3% CONTROL, 7% IDAL, 39% IM). The day after vaccination, IDAL piglets did not differ from the control piglets for any of the behavioural variables studied through scan samplings. IM piglets showed a lower frequency of social negative interactions (p = 0.001) and rope manipulation (p = 0.04) compared to the CONTROL group. Resting postures did not differ between treatments. At 28 h post-vaccination, IDAL piglets presented lower blood C-reactive protein levels (CONTROL = 20 μg/mL; IDAL = 39 μg/mL; IM = 83 μg/mL, p < 0.0001) and blood Haptoglobin (CONTROL = 1.8 mg/mL; IDAL = 1.9 mg/mL vs. IM = 3.1 mg/mL, p < 0.0001) compared to IM piglets. Salivary chromogranin A and alpha-amylase did not differ between treatment groups when measured 25 min post-vaccination. The method of vaccination did not affect the growth of the piglets or their rectal temperature. These results support that needle-free intradermal vaccination reduces vaccination-related pain in growing pigs.
Evaluation of Changes in the Levels of Firmicutes and Bacteroidetes Phyla of Sheep Feces Depending on the Breed
Authors: Cholewińska Paulina , Wołoszyńska Magdalena , Michalak Marta , Czyż Katarzyna , Rant Witold , Janczak Marzena
Published: October 2020

Studies carried out so far have indicated the effect of the microbiome on the composition of ruminant products. Recent studies have shown that not only diet, but also genetic factors can affect the microbiological composition of the digestive system. The aim of the study was to determine the differences in the levels of selected bacterial phyla in terms of breed differences. Three sheep breeds, i.e., Olkuska, Romanov, and old-type Polish Merino, differing in their use (meat–wool, meat, prolificacy) and country of breed origin were included in the study. Sheep at the same age and of the same sex were kept for a period of 3 months in the same environmental conditions and fed the same feed in the same proportions. The study included real-time PCR (polymerase chain reaction) analysis of feces collected before the slaughter and measurements of body weight and chilled carcasses. The obtained results showed significant differences between the breeds in the levels of bacterial populations tested. There were also differences in body weight between the breeds during the first weight measurements, however, the final results did not show any differences—after three months of maintenance all of them reached similar body weights, despite differences in fecal microbiological composition. The study suggests that in addition to diet and environmental conditions, the microbiology can also be influenced by breed.
A Novel Prefix Cache with Two-Level Bloom Filters in IP Address Lookup
Authors: Kim Junghwan , Ko Myeong-Cheol , Shin Moon Sun , Kim Jinsoo
Published: October 2020

Prefix caching is one of the notable techniques in enhancing the IP address lookup performance which is crucial in packet forwarding. A cached prefix can match a range of IP addresses, so prefix caching leads to a higher cache hit ratio than IP address caching. However, prefix caching has an issue to be resolved. When a prefix is matched in a cache, the prefix cannot be the result without assuring that there is no longer descendant prefix of the matching prefix which is not cached yet. This is due to the aspect of the IP address lookup seeking to find the longest matching prefix. Some prefix expansion techniques avoid the problem, but the expanded prefixes occupy more entries as well as cover a smaller range of IP addresses. This paper proposes a novel prefix caching scheme in which the original prefix can be cached without expansion. In this scheme, for each prefix, a Bloom filter is constructed to be used for testing if there is any matchable descendant. The false positive ratio of a Bloom filter generally grows as the number of elements contained in the filter increases. We devise an elaborate two-level Bloom filter scheme which adjusts the filter size at each level, to reduce the false positive ratio, according to the number of contained elements. The experimental result shows that the proposed scheme achieves a very low cache miss ratio without increasing the number of prefixes. In addition, most of the filter assertions are negative, which means the proposed prefix cache effectively hits the matching prefix using the filter.
Control Performances of Friction Pendulum and Sloped Rolling-Type Bearings Designed with Single Parameters
Authors: Wang Shiang-Jung , Sung Yi-Lin , Yang Cho-Yen , Lin Wang-Chuen , Yu Chung-Han
Published: October 2020

Owing to quite different features and hysteretic behavior of friction pendulum bearings (FPBs) and sloped rolling-type bearings (SRBs), their control performances might not be readily compared without some rules. In this study, first, on the premise of retaining the same horizontal acceleration control performance, the effects arising from different sloping angles and damping forces on the horizontal maximum and residual displacement responses of SRBs are numerically examined. For objective comparison of passive control performances of FPBs and SRBs, then, some criteria are considered to design FPBs with the same horizontal acceleration control performance by referring to the designed damping force and the maximum horizontal displacement response of SRBs under a given seismic demand. Based on the considered criteria, the passive control performances of FPBs and SRBs under a large number of far-field and pulse-like near-fault ground motions are quantitatively compared. The numerical comparison results indicate that the FPB models might potentially have better horizontal acceleration and isolation displacement control performances than the SRB models regardless of whether they are subjected to far-field or near-fault ground motions, while the opposite tendency is observed for their self-centering performances, especially when the SRB model designed with a larger sloping angle or a smaller damping force.
The Microstructure Evolution and Mass Transfer in Mushy Zone during High-Pressure Solidifying Hypoeutectic Al-Ni Alloy
Authors: Wang Xiaohong , Dong Duo , Zhu Dongdong , Wang Hongwei , Wei Zunjie
Published: October 2020

The mushy zone of hypoeutectic Al-1.5 wt% Ni alloy during high-pressure synthesis was obtained by changing the structure of the graphite heater. Meanwhile, the evolution of the microstructure was investigated. The results demonstrated that three distinguished zones were successfully generated along with the direction of the temperature gradient, including the fully melted area consisting of the columnar dendrite. The mushy zone is composed of an α-Al phase, bulk β-Al3Ni phase, and a eutectic microstructure as well as a non-melted, solid region. In addition, the mass transfer velocity and the time required for the liquid pool to migrate through the mushy zone during thermal-stable treatment under different high pressures were also analyzed. The results showed that the mass transfer was greatly inhibited, and the minimum time required for a liquid droplet to go through the whole mushy zone at 1 GPa and 3 GPa was 746 h and 5523 h, respectively.
Area-Efficient Short-Time Fourier Transform Processor for Time–Frequency Analysis of Non-Stationary Signals
Authors: Jeon Hohyub , Jung Yongchul , Lee Seongjoo , Jung Yunho
Published: October 2020

In this paper, we propose an area-efficient short-time Fourier transform (STFT) processor that can perform time–frequency analysis of non-stationary signals in real time, which is essential for voice or radar-signal processing systems. STFT processors consist of a windowing module and a fast Fourier transform processor. The length of the window function is related to the time–frequency resolution, and the required window length varies depending on the application. In addition, the window function needs to overlap the input data samples to minimize the data loss in the window boundary, and overlap ratios of 25%, 50%, and 75% are generally used. Therefore, the STFT processor should ideally support a variable window length and overlap ratio and be implemented with an efficient hardware architecture for real-time time–frequency analysis. The proposed STFT processor is based on the radix-4 multi-path delay commutator (R4MDC) pipeline architecture and supports a variable length of 16, 64, 256, and 1024 and overlap ratios of 25%, 50%, and 75%. Moreover, the proposed STFT processor can be implemented with very low complexity by having a relatively lower number of delay elements, which are the ones that increase complexity in the most STFT processors. The proposed STFT processor was designed using hardware description language (HDL) and synthesized to gate-level circuits using a standard cell library in a 65 nm CMOS process. The proposed STFT processor results in logic gates of 197,970, which is 63% less than that of the conventional radix-2 single-path delay feedback (R2SDF) based STFT processor.
Flat Field Soft X-ray Spectrometry with Reflection Zone Plates on a Curved Substrate
Authors: Probst Jürgen , Braig Christoph , Erko Alexei
Published: October 2020

We report on the first experimental results obtained with a newly designed instrument for high-resolution soft X-ray spectroscopy, using reflection zone plates (RZPs) on a spherical substrate. The spectrometer was tested with a fluorescence source. High-resolution flat field spectra within ±50% around the design energies were measured at an interval of 150–750 eV, using only two RZPs: the first RZP, with its design energy of 277 eV, covered the band of 150–370 eV, and the second RZP, with a design energy of 459 eV, covered the band of 350–750 eV, where the upper boundary of this energy range was defined by the Ni coating of the RZPs. The absolute quantum efficiency of the spectrometer, including the optical element and the detector, was, on average, above 10%, and reached 20% at the designed energies of the RZPs. The resolving power E/∆E exceeded 600 for energies E inside the core range of 200–550 eV.
Multi-Frame Labeled Faces Database: Towards Face Super-Resolution from Realistic Video Sequences
Authors: Rajnoha Martin , Mezina Anzhelika , Burget Radim
Published: October 2020

Forensically trained facial reviewers are still considered as one of the most accurate approaches for person identification from video records. The human brain can utilize information, not just from a single image, but also from a sequence of images (i.e., videos), and even in the case of low-quality records or a long distance from a camera, it can accurately identify a given person. Unfortunately, in many cases, a single still image is needed. An example of such a case is a police search that is about to be announced in newspapers. This paper introduces a face database obtained from real environment counting in 17,426 sequences of images. The dataset includes persons of various races and ages and also different environments, different lighting conditions or camera device types. This paper also introduces a new multi-frame face super-resolution method and compares this method with the state-of-the-art single-frame and multi-frame super-resolution methods. We prove that the proposed method increases the quality of face images, even in cases of low-resolution low-quality input images, and provides better results than single-frame approaches that are still considered the best in this area. Quality of face images was evaluated using several objective mathematical methods, and also subjective ones, by several volunteers. The source code and the dataset were released and the experiment is fully reproducible.