bluuebunny/crossref_metadata_2025 huggingface dataset:
Chloride Ions, Vascular Function and Hypertension
Authors: Goto Kenichi , Kitazono Takanari
Published: September 2022

Blood pressure is determined by cardiac output and systemic vascular resistance, and mediators that induce vasoconstriction will increase systemic vascular resistance and thus elevate blood pressure. While peripheral vascular resistance reflects a complex interaction of multiple factors, vascular ion channels and transporters play important roles in the regulation of vascular tone by modulating the membrane potential of vascular cells. In vascular smooth muscle cells, chloride ions (Cl−) are a type of anions accumulated by anion exchangers and the anion–proton cotransporter system, and efflux of Cl− through Cl− channels depolarizes the membrane and thereby triggers vasoconstriction. Among these Cl− regulatory pathways, emerging evidence suggests that upregulation of the Ca2+-activated Cl− channel TMEM16A in the vasculature contributes to the increased vascular contractility and elevated blood pressure in hypertension. A robust accumulation of intracellular Cl− in vascular smooth muscle cells through the increased activity of Na+–K+–2Cl− cotransporter 1 (NKCC1) during hypertension has also been reported. Thus, the enhanced activity of both TMEM16A and NKCC1 could act additively and sequentially to increase vascular contractility and hence blood pressure in hypertension. In this review, we discuss recent findings regarding the role of Cl− in the regulation of vascular tone and arterial blood pressure and its association with hypertension, with a particular focus on TMEM16A and NKCC1.
Transcriptomic Harmonization as the Way for Suppressing Cross-Platform Bias and Batch Effect
Authors: Borisov Nicolas , Buzdin Anton
Published: September 2022

(1) Background: Emergence of methods interrogating gene expression at high throughput gave birth to quantitative transcriptomics, but also posed a question of inter-comparison of expression profiles obtained using different equipment and protocols and/or in different series of experiments. Addressing this issue is challenging, because all of the above variables can dramatically influence gene expression signals and, therefore, cause a plethora of peculiar features in the transcriptomic profiles. Millions of transcriptomic profiles were obtained and deposited in public databases of which the usefulness is however strongly limited due to the inter-comparison issues; (2) Methods: Dozens of methods and software packages that can be generally classified as either flexible or predefined format harmonizers have been proposed, but none has become to the date the gold standard for unification of this type of Big Data; (3) Results: However, recent developments evidence that platform/protocol/batch bias can be efficiently reduced not only for the comparisons of limited transcriptomic datasets. Instead, instruments were proposed for transforming gene expression profiles into the universal, uniformly shaped format that can support multiple inter-comparisons for reasonable calculation costs. This forms a basement for universal indexing of all or most of all types of RNA sequencing and microarray hybridization profiles; (4) Conclusions: In this paper, we attempted to overview the landscape of modern approaches and methods in transcriptomic harmonization and focused on the practical aspects of their application.
Level of Amyloid-β (Aβ) Binding Leading to Differential Effects on Resting State Functional Connectivity in Major Brain Networks
Authors: Cheung Eva Y. W. , Chau Anson C. M. , Shea Yat-Fung , Chiu Patrick K. C. , Kwan Joseph S. K. , Mak Henry K. F.
Published: September 2022

Introduction: Amyloid-β protein (Aβ) is one of the biomarkers for Alzheimer’s disease (AD). The recent application of interhemispheric functional connectivity (IFC) in resting-state fMRI has been used as a non-invasive diagnostic tool for early dementia. In this study, we focused on the level of Aβ accumulated and its effects on the major functional networks, including default mode network (DMN), central executive network (CEN), salience network (SN), self-referential network (SRN) and sensory motor network (SMN). Methods: 58 participants (27 Hi Aβ (HiAmy) and 31 low Aβ (LowAmy)) and 25 healthy controls (HC) were recruited. [18F]flutemetamol PET/CT was performed for diseased groups, and MRI scanning was done for all participants. Voxel-by-voxel correlation analysis was done for both groups in all networks. Results: In HiAmy, IFC was reduced in all networks except SN. A negative correlation in DMN, CEN, SRN and SMN suggests high Aβ related to IFC reduction; However, a positive correlation in SN suggests high Aβ related to an increase in IFC. In LowAmy, IFC increased in CEN, SMN, SN and SRN. Positive correlation in all major brain networks. Conclusion: The level of Aβ accumulated demonstrated differential effects on IFC in various brain networks. As the treatment to reduce Aβ plaque deposition is available in the market, it may be an option for the HiAmy group to improve their IFC in major brain networks.
Retinol Binding Protein, Sunlight Hours, and the Influenza Virus-Specific Immune Response
Authors: Patel Nehali , Penkert Rhiannon R. , Sealy Robert E. , Surman Sherri L. , Jones Bart G. , Ringwald-Smith Karen , Ross A. Catharine , Hurwitz Julia L.
Published: September 2022

Healthy pediatric immune responses depend on adequate vitamin A and D levels. Relationships between solar ultraviolet B (UVB) radiation and vitamin D are well understood, while relationships between sunlight, vitamin A, and its serum escort, retinol binding protein (RBP), are not. A pediatric clinical study enrolled 2–8-year-old children at various times between September 2016 and March 2017, inclusive, in Memphis, Tennessee. A serum sample from each child was then assayed to examine the influence of season on vitamin levels. We found that RBP and RBP/retinol molar ratios decreased in winter months and RBP/retinol ratios correlated positively with the average daily sunlight hours per month. A food frequency questionnaire given to parents/guardians indicated a shift in dietary intake from plant-based foods to animal-based foods by children between winter and spring months. This translated to higher retinol and zinc (integral to RBP–transthyretin–retinol complexes) in the spring, perhaps explaining the seasonal influence on RBP/retinol. RBP and retinol were associated positively with IgG/IgM and IgA/IgM ratios. RBP and retinol, but not 25(OH)D, also correlated positively with influenza virus-specific antibodies. Retinol correlated negatively, while 25(OH)D correlated positively, with certain serum cytokine/chemokine levels. Significant differences in 25(OH)D, immunoglobulin ratios, and cytokines/chemokines were observed between black and white children. In sum, seasonal changes in dietary foods rich in retinol and zinc may have influenced RBP levels, which in turn influenced innate and adaptive immune responses. Results encourage routine monitoring and reporting of season, RBP, and vitamin levels in future clinical studies, as seasons may affect sunlight exposures, diet, vitamin levels, and immune protection against infectious disease.
A Biomimetic Basalt Fiber/Epoxy Helical Composite Spring with Hierarchical Triple-Helix Structures Inspired by the Collagen Fibers in Compact Bone
Authors: Wang Jiahui , Shi Zhongyuan , Han Qigang , Sun Yanbiao , Shi Mingdi , Li Rui , Wei Rubin , Dong Bin , Zhai Wen , Zheng Wenfang , Li Yueying , Chen Nuo
Published: September 2022

The lightweight property of helical composite spring (HCS) applied in the transportation field has attracted more and more attention recently. However, it is difficult to maintain stiffness and fatigue resistance at the same time. Herein, inspired by collagen fibers in bone, a bionic basalt fiber/epoxy resin helical composite spring is manufactured. The collagen fibers consist of nanoscale hydroxyapatite (increases stiffness) and collagen molecules composed of helical amino acid chains (can increase fatigue resistance). Such a helical structure of intercalated crystals ensures that bone has good resistance to fracture. Specifically, we first investigated the effect of adding different contents of NS to basalt fibers on the stiffness and fatigue properties of an HCS. The results show that the optimal NS content of 0.4 wt% resulted in 52.1% and 43.5% higher stiffness and fatigue properties of an HCS than those without NS, respectively. Then, two braided fiber bundles (TS-BFB) and four braided fiber bundles (FS-BFB) were designed based on the helical structure of amino acid chains, and the compression tests revealed that the maximum load resistance of TS-BFB and FS-BFB was increased by 29.2% and 44%, respectively, compared with the conventional single fiber bundle (U-BFB). The superior mechanical performance of TS-BFB and FS-BFB is attributed to the more adequate bonding of 0.4 wt% NS to the epoxy resin and the multi-fiber bundles that increase the transverse fiber content of the spring. The findings in this work introduce the bionic collagen fiber structure into the design for an HCS and provide a new idea to improve the spring performance.
Fibonacci Sequences, Symmetry and Order in Biological Patterns, Their Sources, Information Origin and the Landauer Principle
Authors: Bormashenko Edward
Published: September 2022

Physical roots, exemplifications and consequences of periodic and aperiodic ordering (represented by Fibonacci series) in biological systems are discussed. The physical and biological roots and role of symmetry and asymmetry appearing in biological patterns are addressed. A generalization of the Curie–Neumann principle as applied to biological objects is presented, briefly summarized as: “asymmetry is what creates a biological phenomenon”. The “top-down” and “bottom-up” approaches to the explanation of symmetry in organisms are presented and discussed in detail. The “top-down” approach implies that the symmetry of the biological structure follows the symmetry of the media in which this structure is functioning; the “bottom-up” approach, in turn, accepts that the symmetry of biological structures emerges from the symmetry of molecules constituting the structure. A diversity of mathematical measures applicable for quantification of order in biological patterns is introduced. The continuous, Shannon and Voronoi measures of symmetry/ordering and their application to biological objects are addressed. The fine structure of the notion of “order” is discussed. Informational/algorithmic roots of order inherent in the biological systems are considered. Ordered/symmetrical patterns provide an economy of biological information, necessary for the algorithmic description of a biological entity. The application of the Landauer principle bridging physics and theory of information to the biological systems is discussed.
Recent Advances in Nanomechanical Membrane-Type Surface Stress Sensors towards Artificial Olfaction
Authors: Minami Kosuke , Imamura Gaku , Tamura Ryo , Shiba Kota , Yoshikawa Genki
Published: September 2022

Nanomechanical sensors have gained significant attention as powerful tools for detecting, distinguishing, and identifying target analytes, especially odors that are composed of a complex mixture of gaseous molecules. Nanomechanical sensors and their arrays are a promising platform for artificial olfaction in combination with data processing technologies, including machine learning techniques. This paper reviews the background of nanomechanical sensors, especially conventional cantilever-type sensors. Then, we focus on one of the optimized structures for static mode operation, a nanomechanical Membrane-type Surface stress Sensor (MSS), and discuss recent advances in MSS and their applications towards artificial olfaction.
A Highly Integrated and Diminutive Fluorescence Detector for Point-of-Care Testing: Dual Negative Feedback Light-Emitting Diode (LED) Drive and Photoelectric Processing Circuits Design and Implementation
Authors: Wang Yue , Fang Yile , Liu Haoran , Su Xiangyi , Chen Zhu , Li Song , He Nongyue
Published: September 2022

As an important detection tool in biochemistry, fluorescence detection has wide applications. Quantitative detection can be achieved by detecting fluorescence signals excited by excitation light at a specific wavelength range. Therefore, the key to fluorescence detection is the stable control of the excitation light and the accurate acquisition of weak photoelectric signals. Moreover, to improve portability and instantaneity, devices are developing in miniaturization and integration. As the core of such devices, fluorescence detectors should also have these features. Under this circumstance, we designed a highly integrated and diminutive fluorescence detector and focused on its excitation light driving and photoelectric signal processing. A current–light dual negative feedback light-emitting diode (LED) driving circuit was proposed to obtain constant current and luminance. In addition, a silicon photodiode (PD) was used to receive and convert the fluorescence signal to an electric signal. Then, amplifying, filtering, and analog-to-digital (A/D) converting were applied to make the detection of weak fluorescence signals possible. The test results showed that the designed circuit has wonderful performance, and the detector shows good linearity (R2 = 0.9967) and sensitivity (LOD = 0.077 nM) in the detection of fluorescein sodium solution. Finally, a real-time fluorescence polymerase chain reaction (real-time PCR) of Legionella pneumophila was carried out on a homemade platform equipped with this detector, indicating that the detector met the requirements of real-time PCR detection.
Escherichia coli Enumeration in a Capillary-Driven Microfluidic Chip with SERS
Authors: Dogan Üzeyir , Sucularlı Ferah , Yildirim Ender , Cetin Demet , Suludere Zekiye , Boyaci Ismail Hakkı , Tamer Ugur
Published: September 2022

Pathogen detection is still a challenging issue for public health, especially in food products. A selective preconcentration step is also necessary if the target pathogen concentration is very low or if the sample volume is limited in the analysis. Plate counting (24–48 h) methods should be replaced by novel biosensor systems as an alternative reliable pathogen detection technique. The usage of a capillary-driven microfluidic chip is an alternative method for pathogen detection, with the combination of surface-enhanced Raman scattering (SERS) measurements. Here, we constructed microchambers with capillary microchannels to provide nanoparticle–pathogen transportation from one chamber to the other. Escherichia coli (E. coli) was selected as a model pathogen and specific antibody-modified magnetic nanoparticles (MNPs) as a capture probe in a complex milk matrix. MNPs that captured E. coli were transferred in a capillary-driven microfluidic chip consisting of four chambers, and 4-aminothiophenol (4-ATP)-labelled gold nanorods (Au NRs) were used as the Raman probe in the capillary-driven microfluidic chip. The MNPs provided immunomagnetic (IMS) separation and preconcentration of analytes from the sample matrix and then, 4-ATP-labelled Au NRs provided an SERS response by forming sandwich immunoassay structures in the last chamber of the capillary-driven microfluidic chip. The developed SERS-based method could detect 101–107 cfu/mL of E. coli with the total analysis time of less than 60 min. Selectivity of the developed method was also tested by using Salmonella enteritidis (S. enteritidis) and Staphylococcus aureus (S. aureus) as analytes, and very weak signals were observed.
A Study on the Mediating Role of Emotional Solidarity between Authenticity Perception Mechanism and Tourism Support Behavior Intentions within Rural Homestay Inn Tourism
Authors: Chen Jie , Liu Chang , Si Yuqi , Law Rob , Zhang Mu
Published: September 2022

Rural homestay inns are an important part of rural tourism, and tourists’ support behavior intentions are important factors affecting whether rural homestay inns can be developed sustainably. The local authentic life experiences and realization of actual communication between the host and tourists are the main influencing factors for tourists to revisit, recommend, or provide support. Although previous studies have confirmed the influence of authenticity perception on tourists’ support behavior intentions from different perspectives, they have not analyzed the influence mechanism between them from the perspective of micro interpersonal emotional attitude. To further understand the impact mechanism between the two, this study introduces the variable of emotional solidarity; constructs a relationship model of authenticity perception, emotional solidarity, and tourists’ support behavior intentions by adopting the theory of reasoned action; and verifies the established hypotheses through empirical analysis. The results show that both existential authenticity and objective authenticity positively influence tourism support behavior intentions, and the effect of objective authenticity on tourism support behavior intentions is greater than that of the presence of authenticity. Empathic understanding, feeling welcome, and emotional intimacy all play mediating roles between intrapersonal authenticity perception and tourism support behavior intentions. Findings also show empathic understanding and feeling welcome play mediating roles in objective authenticity perception and between the perception of objective authenticity and tourism support behavior intentions. Suggestions are also proposed for the development of homestay inn enterprises.
Perceived Physical Education Teachers’ Controlling Behaviour and Students’ Physical Activity during Leisure Time—The Dark Side of the Trans-Contextual Model of Motivation
Authors: Viksi Andres , Tilga Henri
Published: September 2022

Background: Previous studies have shown that the controlling behaviour of physical education teachers might be detrimental to their students’ psychological experiences. The purpose of this work was to examine whether and to what extent the different dimensions of the perceived controlling teaching questionnaire predict students’ basic psychological needs, motivations, and physical activities during leisure-time. Methods: A total of 299 students (164 boys and 135 girls) from four Estonian general education schools and two vocational education institutions participated in the study. Students filled in the questionnaire of study variables. A variance-based structural model was used to test the research hypotheses. Results: The results revealed that different forms of controlling behaviours predicted psychological need frustration (β = 0.09–0.37; p < 0.01). Psychological need frustration predicted controlled motivation (β = 0.52; p < 0.01). Controlled motivation predicted subjective norms (β = 0.51; p < 0.01). Intention was predicted by attitudes (β = 0.30; p < 0.01), perceived behavioural control (β = 0.37; p < 0.01), and subjective norms (β = 0.15; p < 0. 01). Attitude was statistically significantly related to leisure-time physical activity (β = 0.09; p < 0.05). The model describes 10% of students’ physical activity in the context of leisure-time. Conclusion: The results of this study highlight that physical education teachers should avoid using controlling behaviours if the aim is to avoid frustrating their students’ psychological needs, which might have detrimental effect on students’ leisure-time physical activity via controlled forms of motivation.
A Field Study of Outdoor Human Thermal Perception in Three Seasons in Shanghai, China
Authors: Wei Dongxue , Lian Zefeng , Liu Binyi
Published: September 2022

The locality of landscape design needs to obtain human thermal perception in different cities or regions. Previous studies in Shanghai have focused on a single season and ignored the seasonal characteristics of hot summers and cold winters. The objective of this research was to examine the outdoor human thermal perception in three seasons—summer, autumn, and winter. For this purpose, a field survey was conducted in two urban squares in Shanghai, and during three seasons, an outdoor human thermal perception questionnaire survey was completed combined with micrometeorological measurements. In the questionnaire, the thermal sensation and thermal comfort of interviewees were evaluated through the 9-point scale and the 4-point break scale, respectively. Regression lines gave the possibility to calculate the neutral ranges and the comfort ranges. A neutral PET (physiological equivalent temperature) range of 13.0~28.6 °C and a comfort PET range of 14.2~32.6 °C were obtained for three seasons. In addition, the relationships between personal (gender and age of respondents) and microclimatic (air temperature, relative humidity, wind velocity, and solar radiation) factors on thermal sensation votes (TSV) and thermal comfort votes (TCV) were evaluated. The results of multiple regression analysis showed that the variables affecting TSV and TCV changed with the season. The findings of this research enhance our understanding of outdoor human thermal perception and can contribute to more friendly outdoor open space design in Shanghai.
Study on Seismic Performance of TID-LRB Hybrid Control System under Multi-Level Earthquakes
Authors: Huang Xiao , Hu Zhixiang , Liu Yunlin , Nie Liqing
Published: September 2022

The seismic response characteristics of a lead-rubber-bearing(LRB) base-isolated structure under rare and very rare earthquakes were investigated. The acceleration, ductility coefficient, and shear strain of the LRB increase significantly under very rare earthquakes in comparison to rare earthquakes; in particular, the shear strain of the LRB may exceed the ultimate shear strain and cause damage to the base-isolated structure. The criterion selected for the optimum tuned inerter damper (TID) of the TID–LRB hybrid control system is the minimization of the mean value of the maximum shear strain of the LRB. For each inertance mass ratio of the TID, there exists an optimum tuning frequency ratio and damping ratio of the TID to minimize the shear strain of the LRB, and the effectiveness is increased with a higher inertance mass ratio. By equipping the TID with appropriate parameters, the safety of the LRB during rare and very rare earthquakes can be ensured. Finally, the pounding response of the base-isolated structure collision with the moat wall under very rare earthquakes was analyzed. It was observed that under very rare earthquakes, the ductility coefficients of the superstructure by equipping with the suitable TID were improved, and the shear strain of the LRB was reduced. In addition, equipping the TID can reduce the required width of the isolation joint to avoid collision between the isolation layer and the moat wall, and with an increase in the inertance mass ratio, the required width of the isolation joint is smaller.
Production of the Traditional Organic Mortars of Padmanabhapuram Palace—A Characterization Study on the Simulated Mortars for Their Compatibility
Authors: Shivakumar Mani , Singh Aruna , Selvaraj Thirumalini , Thangaraj Shanmugapriya
Published: September 2022

The scientific investigation performed on the different mortar typologies of the Padmanabhapuram Palace mortars in a previous study led to the formulation of a similar traditional mortar. The outcome of the study was an increase in the carbonation action and mechanical strength of the mortar compared to conventional lime mortar, primarily due to the fermented organics such as aloe vera, cactus, kadukkai, hibiscus, jaggery, and neelamari. The transformation of portlandite crystals to calcite, along with the early developed C-S-H and C-A-S-H hydrated products reported by the XRD analysis. The bio-organic spectral peaks for compounds such as carbohydrates, polysaccharides, and fatty acids were observed in the FT-IR investigation, which corroborates the XRD mineralogical results. The calcite decomposition is detected in the TGA analysis in the temperature range of 700–750 °C, with a maximum weight loss of approximately 35–37% for the aloe vera lime mortar. The addition of fermented organic extracts to the extent of 5% was found to increase the internal and external carbon absorption of the aloe vera and cactus mortars compared to conventional lime mortars. The mechanical strength of the organic lime mortar reported as 2.5 MPa, and 1.5 MPa for the reference mortar. The carbonation and hydraulic reactions due to the presence of polysaccharides, fatty acids, carbohydrates, and proteins in the formulation enhanced the compressive strength of the compatible mortar. The prepared compatible mortar will support the revival of the 400-year-old practice of the application of Palace mortars.
Study on Mix Proportion Design Based on Strength and Sulfate Resistance of 100% Recycled Aggregate Concrete
Authors: Li Haizhou , Liu Jianping , Chu Fujiao , Zhang Lu
Published: September 2022

This paper presents the mechanical properties of 100% recycled aggregate concrete (RAC), and the results and analysis of the dry–wet cycle accelerated sulfate attack test. The results show that recycled concrete aggregate (RCA) can replace the coarse and fine aggregate. The recycled clay brick aggregate (RCBA) is not suitable for use as a coarse aggregate because the water absorption exceeds the standard. RCA replaces the coarse aggregate; and RCBA returns the fine aggregate to prepare 100% recycled concrete aggregate (RAC). The water–cement ratio is the most significant factor affecting the compressive strength of 100% RAC. The results of the mechanical properties analysis show that the compressive strength of RAC is less than that of NAC, and the difference in compressive strength between 100% RAC and NAC decreases with age. The splitting tensile strength of 100% RAC was slightly higher than that of NAC except for 7 d. The results of the dry–wet cycle accelerated sulfate attack test showed that the performance of 100% RAC was lower than that of NAC under the dry–wet process and sulfate attack coupling. Still, the loss rate was less than 5%, which met the standard resistance to the dry–wet cycle accelerating the sulfate attack.
Case Study of the Application of an Innovative Guide for the Seismic Vulnerability Evaluation of Schools Located in Sangolquí, Interandean Valley in Ecuador
Authors: Ballesteros-Salazar Kevin Sebastián , Caizaguano-Montero Diego German , Haro-Báez Ana Gabriela , Toulkeridis Theofilos
Published: September 2022

The current study is based on the analysis and adaptation of a Federal Emergency Management Agency guide, FEMA P-1000, from the USA to improve school safety against natural hazards by applying the guide to the infrastructure of Ecuadorian schools, focusing primarily on seismic risk. By considering the technical foundations of structuring and managing disasters in buildings for school use, society will be provided with a practical procedure to recognize those aspects that need immediate attention as part of proper risk management. Here, a variety of parameters are involved in the proposed methodology of the given guide from FEMA combined with the national construction standards and regulations. The characteristics of nearby geological faults and structural and nonstructural vulnerability levels, amongst others, were also considered to allow for a detailed evaluation and a subsequent seismic risk categorization. Finally, the global risk is determined for the studied institutions of Sangolquí in the Valley of Los Chillos, within the Interandean Depression in central Ecuador.
BIM-Based Resource Tradeoff in Project Scheduling Using Fire Hawk Optimizer (FHO)
Authors: Shishehgarkhaneh Milad , Azizi Mahdi , Basiri Mahla , Moehler Robert
Published: September 2022

Project managers should balance a variety of resource elements in building projects while taking into account many major concerns, including time, cost, quality, risk, and the environment. This study presents a framework for resource trade-offs in project scheduling based on the Building Information Modeling (BIM) methodology and metaheuristic algorithms. First, a new metaheuristic algorithm called Fire Hawk Optimizer (FHO) is used. Using project management software and the BIM process, a 3D model of the construction is created. In order to maximize quality while minimizing time, cost, risk, and CO2 in the project under consideration, an optimization problem is created, and the FHO’s capability for solving it is assessed. The results show that the FHO algorithm is capable of producing competitive and exceptional outcomes when it comes to the trade-off of various resource options in projects.
Multi-Objective Optimisation of Urban Form: A Framework for Selecting the Optimal Solution
Authors: Showkatbakhsh Milad , Makki Mohammed
Published: September 2022

The complexity associated with the design of urban tissues is driven by the multitude of design goals that influence urban development and growth. This complexity is amplified by the design goals being inherently conflicting, necessitating preference-based decisions within the design process—an approach that results in predetermined design solutions driven by personal biases. The utility of population-based optimisation algorithms addresses this by allowing for the examination of multiple conflicting objectives within the same design problem, negating the need for trade-off decisions between the design goals. The application of these algorithms is associated with three primary steps. The first is the formulation of the design problem, the second is the application of the algorithm, and the third is selecting the most optimal solution from the algorithm’s output. This paper examines the third step in this process, in which various methods are employed to facilitate data-driven selection mechanisms that are both objective as well as subjective in their formulation. The selection mechanisms are demonstrated on a speculative urban tissue that examines the potential of inhabiting interstitial spaces, through various morphological interventions, within the urban fabric. The results present a scalable and adaptable framework that assists designers employing multi-objective evolutionary algorithms (MOEAs) to select the optimal solution from their generated populations, a challenge commonly associated with the application of MOEAs in design.
Measuring Work Autonomy and Its Role in Enhancing Labour Productivity: The Case of the Vietnamese Construction Industry
Authors: Tam Nguyen Van , Watanabe Tsunemi , Hai Nguyen Luong
Published: September 2022

Work autonomy (WA) is an important factor in improving work performance, yet such freedom and its role in construction workforce management have rarely been discussed. To address this gap, this study quantitatively measured the WA of onsite construction workers in Vietnam, developed a new model by which to discover the function of WA in increasing construction labour productivity (CLP) and determined the ways through which WA can be cultivated and maintained. The WA of 215 workers was measured using the relative autonomy index (RAI) and an aggregated motivation index (AMI) that was developed in this research. Structural equation modelling (SEM) was conducted to examine the effects of WA on CLP. The SEM results indicated that WA positively and significantly contributed to CLP. Promoting WA required paying attention to the competence and relatedness satisfaction of the workers. Furthermore, latent and potentially extensive labour management-related problems were identified, namely, unsuccessful career development and the underutilisation of experienced workers. Three meaningful policy recommendations were put forward to solve the aforementioned problems and improve CLP: the effective organisation of crew members, the improvement of training and the improvement of site amenities. This study expands theoretical knowledge by (1) developing and justifying the AMI as an auxiliary to conventional indices, (2) proposing five conditions necessary for optimal scoring in WA measurement and (3) developing a motivation matrix that identifies and distinguishes the attributes of different groups. In practical terms, the findings support the introduction of reasonable policies that advance the career development of workers, promote WA and improve CLP. These achievements, in turn, significantly advance effective and sustainable construction workforce management.
CO2 Methanation of Biogas over Ni-Mg-Al: The Effects of Ni Content, Reduction Temperature, and Biogas Composition
Authors: Han Danbee , Cho Wonjun , Baek Youngsoon
Published: September 2022

Biogas is mainly composed of CH4 and CO2, so it is used as an alternative energy to CH4 with high energy density by separating and removing CO2 from biogas. In addition, it can be utilized by producing synthesis gas (CO and H2) through thermal decomposition of biogas or by synthesizing CH4 by methanation of CO2. The technique of CO2 methanation is a method that can improve the CH4 concentration without CO2 separation. This study aims to produce more efficient methane through CO2 methanation of biogas over Ni-Mg-Al catalyst. So, the effect of Ni contents in catalyst, catalyst reduction temperature, CO2 concentration in biogas, and the initial concentration of CH4 on CO2 conversion rate and CH4 selectivity was investigated. In addition, the effect of increasing CO2 concentration, H2/CO2 ratio, and GHSV (gas space velocity per hour) on H2 conversion, CH4 productivity, and product was investigated. In particular, the durability and stability of CO2 methanation was tested over 60 wt% Ni-Mg-Al catalyst at 350 °C and 30,000/h for 130 h. From the long-term test results, the catalyst shows stability by maintaining a constant CO2 conversion rate of 72% and a CH4 selectivity of 95%.
Se-Doped Ni5P4 Nanocatalysts for High-Efficiency Hydrogen Evolution Reaction
Authors: An Cuihua , Wang Yuchen , Jiao Penggang , Wu Shuai , Gao Lingxiao , Zhu Chunyou , Li Junsheng , Hu Ning
Published: September 2022

Increasing energy consumption and environmental pollution problems have forced people to turn their attention to the development and utilization of hydrogen energy, which requires that hydrogen energy can be efficiently prepared. However, the sluggish kinetics of hydrogen evolution reaction (HER) requires higher overpotential. It is urgent to design and fabricate catalysts to drive the procedure and decrease the overpotential of HER. It is well known that platinum catalysts are the best for HER, but their high cost limits their wide application. Transition metals such as Fe, Co, Mo and Ni are abundant, and transition metal phosphides are considered as promising HER catalysts. Nevertheless, catalysts in powder form are very easily soluble in the electrolyte, which leads to inferior cycling stability. In this work, Ni5P4 anchored on Ni foam was doped with Se powder. After SEM characterization, the Ni5P4-Se was anchored on Ni foam, which circumvents the use of the conductive additives and binder. The Ni5P4-Se formed a porous nanosheet structure with enhanced electron transfer capability. The prepared Ni5P4-Se exhibited high electrochemical performances. At 10 mA cm−2, the overpotential was only 128 mV and the Tafel slope is 163.14 mV dec−1. Additionally, the overpotential was stabilized at 128 mV for 30 h, suggesting its excellent cycling stability. The results show that Se doping can make the two phases achieve a good synergistic effect, which makes the Ni5P4-Se catalyst display excellent HER catalytic activity and stability.
Development of Adsorptive Materials for Selective Removal of Toxic Metals in Wastewater: A Review
Authors: Motitswe Moeng Geluk , Badmus Kassim Olasunkanmi , Khotseng Lindiwe
Published: September 2022

Removal of toxic metals is essential to achieving sustainability in wastewater purification. The achievement of efficient treatment at a low cost can be seriously challenging. Adsorption methods have been successfully demonstrated for possession of capability in the achievement of the desirable sustainable wastewater treatment. This review provides insights into important conventional and unconventional materials for toxic metal removal from wastewater through the adsorption process. The importance of the role due to the application of nanomaterials such as metal oxides nanoparticle, carbon nanomaterials, and associated nanocomposite were presented. Besides, the principles of adsorption, classes of the adsorbent materials, as well as the mechanisms involved in the adsorption phenomena were discussed.
A Review of Persulfate Activation by Magnetic Catalysts to Degrade Organic Contaminants: Mechanisms and Applications
Authors: Tian Ke , Shi Fengyin , Cao Menghan , Zheng Qingzhu , Zhang Guangshan
Published: September 2022

All kinds of refractory organic pollutants in environmental water pose a serious threat to human health and ecosystems. In recent decades, sulfate radical-based advanced oxidation processes (SR-AOPs) have attracted extensive attention in the removal of these organic pollutants due to their high redox potential and unique selectivity. This review first introduces persulfate activation by magnetic catalysts to degrade organic contaminants. We present the advances and classifications in the generation of sulfate radicals using magnetic catalysts. Subsequently, the degradation mechanisms in magnetic catalysts activated persulfate system are summarized and discussed. After an integrated presentation of magnetic catalysts in SR-AOPs, we discuss the application of persulfate activation by magnetic catalysts in the treatment of wastewater, landfill leachate, biological waste sludge, and soil containing organic pollutants. Finally, the current challenges and perspectives of magnetic catalysts that activated persulfate systems are summarized and put forward.
Catalytic Hydrogenation of Nitrocyclohexane with CuCo/SiO2 Catalysts in Gas and Liquid Flow Reactors
Authors: Kowalewski Emil , Matus Krzysztof , Gajek Arkadiusz , Śrębowata Anna
Published: September 2022

Catalytic hydrogenation of nitrocyclohexane proved to be an attractive alternative source of various chemical compounds: cyclohexanone oxime, cyclohexanone, cyclohexanol, cyclohexylamine and dicyclohexylamine. A growing interest in this reaction has been observed in the last few years. Herein, we present the catalytic performance of Cu/SiO2, Co/SiO2 and CuCo/SiO2 in gas and liquid flow nitrocyclohexane hydrogenation. The analysis of synthesized catalysts morphology (BET, TPR, XRD, TEM) in terms of their catalytic behavior allows us to draw general conclusions and determine the optimal conditions for the production of desired products. Application of the monometallic copper leads to the formation of cyclohexanone as the main product, but with low activity. On the other hand, Co/SiO2 shows high activity but gives cyclohexylamine. Bimetallic system CuCo(3:1)/SiO2 allows for the efficient production of 100% cyclohexanone at 5 bar and 75 °C.
A Convenient U-Shape Microreactor for Continuous Flow Biocatalysis with Enzyme-Coated Magnetic Nanoparticles-Lipase-Catalyzed Enantiomer Selective Acylation of 4-(Morpholin-4-yl)butan-2-ol
Authors: Imarah Ali O. , Silva Fausto M. W. G. , Tuba László , Malta-Lakó Ágnes , Szemes József , Sánta-Bell Evelin , Poppe László
Published: September 2022

This study implements a convenient microreactor for biocatalysis with enzymes immobilized on magnetic nanoparticles (MNPs). The enzyme immobilized onto MNPs by adsorption or by covalent bonds was lipase B from Candida antarctica (CaLB). The MNPs for adsorption were obtained by covering the magnetite core with a silica shell and later with hexadecyltrimethoxysilane, while for covalent immobilization, the silica-covered MNPs were functionalized by a layer forming from mixtures of hexadecyl- and 3-(2-aminoethylamino)propyldimethoxymethylsilanes in 16:1 molar ratio, which was further activated with neopentyl glycol diglycidyl ether (NGDE). The resulting CaLB-MNPs were tested in a convenient continuous flow system, created by 3D printing to hold six adjustable permanent magnets beneath a polytetrafluoroethylene tube (PTFE) to anchor the MNP biocatalyst inside the tube reactor. The anchored CaLB-MNPs formed reaction chambers in the tube for passing the fluid through and above the MNP biocatalysts, thus increasing the mixing during the fluid flow and resulting in enhanced activity of CaLB on MNPs. The enantiomer selective acylation of 4-(morpholin-4-yl)butan-2-ol (±)-1, being the chiral alcohol constituent of the mucolytic drug Fedrilate, was carried out by CaLB-MNPs in the U-shape reactor. The CaLB-MNPs in the U-shape reactor were compared in batch reactions to the lyophilized CaLB and to the CaLB-MNPs using the same reaction composition, and the same amounts of CaLB showed similar or higher activity in flow mode and superior activity as compared to the lyophilized powder form. The U-shape permanent magnet design represents a general and easy-to-access implementation of MNP-based flow microreactors, being useful for many biotransformations and reducing costly and time-consuming downstream processes.
Effects of Elamipretide on Autophagy in Renal Cells of Pigs with Metabolic Syndrome
Authors: Hong Siting , Ghandriz Ramyar , Siddiqi Sarosh , Zhu Xiang-Yang , Saadiq Ishran M. , Jordan Kyra L. , Tang Hui , Ali Khaled A. , Lerman Amir , Eirin Alfonso , Lerman Lilach O.
Published: September 2022

Autophagy eliminates excessive nutrients and maintains homeostasis. Obesity and metabolic syndrome (MetS) dysregulate autophagy, possibly partly due to mitochondria injury and inflammation. Elamipretide (ELAM) improves mitochondrial function. We hypothesized that MetS blunts kidney autophagy, which ELAM would restore. Domestic pigs were fed a control or MetS-inducing diet for 16 weeks. During the 4 last weeks, MetS pigs received subcutaneous injections of ELAM (0.1 mg/kg/day, MetS + ELAM) or vehicle (MetS), and kidneys were then harvested to measure protein expression of autophagy mediators and apoptosis. Systemic and renal venous levels of inflammatory cytokines were measured to calculate renal release. The function of isolated mitochondria was assessed by oxidative stress, energy production, and pro-apoptotic activity. MetS slightly downregulated renal expression of autophagy mediators including p62, ATG5-12, mTOR, and AMPK vs. control. Increased mitochondrial H2O2 production accompanied decreased ATP production, elevated apoptosis, and renal fibrosis. In MetS + ELAM, mito-protection restored autophagic protein expression, improved mitochondrial energetics, and blunted renal cytokine release and fibrosis. In vitro, mitoprotection restored mitochondrial membrane potential and reduced oxidative stress in injured proximal tubular epithelial cells. Our study suggests that swine MetS mildly affects renal autophagy, possibly secondary to mitochondrial damage, and may contribute to kidney structural damage in MetS.
Cell Tracking by Magnetic Particle Imaging: Methodology for Labeling THP-1 Monocytes with Magnetic Nanoparticles for Cellular Imaging
Authors: Remmo Amani , Löwa Norbert , Kosch Olaf , Eberbeck Dietmar , Ludwig Antje , Kampen Lena , Grüttner Cordula , Wiekhorst Frank
Published: September 2022

Magnetic particle imaging (MPI) is a noninvasive tomographic imaging modality for the quantitative visualization of magnetic nanoparticles (MNPs) with high temporal and spatial resolution. The general capability of MPI for cell tracking (e.g., monitoring living cells labeled with MNPs) has successfully been shown. MNPs in cell culture media are often subjected to structural and magnetic changes. In addition to the deteriorating reproducibility, this also complicates the systematic study of the relationship between the MNP properties and their cellular uptake for MPI. Here, we present a method for the preparation of magnetically labeled THP-1 (Tamm–Horsfall Protein-1) monocytes that are used in MPI cell tracking. The method development was performed using two different MPI tracers, which exhibited electrostatic and steric stabilizations, respectively. In the first step, the interaction between the MNPs and cell culture media was investigated and adjusted to ensure high structural and magnetic stability. Furthermore, the influences of the incubation time, MNP concentration used for cellular uptake, and individual preparation steps (e.g., the washing of cells) were systematically investigated. Finally, the success of the developed loading method was demonstrated by the MPI measurements. The presented systematic investigation of the factors that influence the MNP loading of cells will help to develop a reliable and reproducible method for MPI monocyte tracking for the early detection of inflammation in the future.
Effects of Cigarette Smoke on Adipose and Skeletal Muscle Tissue: In Vivo and In Vitro Studies
Authors: Wang Lei , van Iersel Lieke E. J. , Pelgrim Charlotte E. , Lu Jingyi , van Ark Ingrid , Leusink-Muis Thea , Gosker Harry R. , Langen Ramon C. J. , Schols Annemie M. W. J. , Argilés Josep M. , van Helvoort Ardy , Kraneveld Aletta D. , Garssen Johan , Henricks Paul A. J. , Folkerts Gert , Braber Saskia
Published: September 2022

Chronic obstructive pulmonary disease (COPD), often caused by smoking, is a chronic lung disease with systemic manifestations including metabolic comorbidities. This study investigates adaptive and pathological alterations in adipose and skeletal muscle tissue following cigarette smoke exposure using in vivo and in vitro models. Mice were exposed to cigarette smoke or air for 72 days and the pre-adipose cell line 3T3-L1 was utilized as an in vitro model. Cigarette smoke exposure decreased body weight, and the proportional loss in fat mass was more pronounced than the lean mass loss. Cigarette smoke exposure reduced adipocyte size and increased adipocyte numbers. Adipose macrophage numbers and associated cytokine levels, including interleukin-1β, interleukine-6 and tumor necrosis factor-α were elevated in smoke-exposed mice. Muscle strength and protein synthesis signaling were decreased after smoke exposure; however, muscle mass was not changed. In vitro studies demonstrated that lipolysis and fatty acid oxidation were upregulated in cigarette smoke-exposed pre-adipocytes. In conclusion, cigarette smoke exposure induces a loss of whole-body fat mass and adipose atrophy, which is likely due to enhanced lipolysis.
Primary Cilia Influence Progenitor Function during Cortical Development
Authors: Zaidi Donia , Chinnappa Kaviya , Francis Fiona
Published: September 2022

Corticogenesis is an intricate process controlled temporally and spatially by many intrinsic and extrinsic factors. Alterations during this important process can lead to severe cortical malformations. Apical neuronal progenitors are essential cells able to self-amplify and also generate basal progenitors and/or neurons. Apical radial glia (aRG) are neuronal progenitors with a unique morphology. They have a long basal process acting as a support for neuronal migration to the cortical plate and a short apical process directed towards the ventricle from which protrudes a primary cilium. This antenna-like structure allows aRG to sense cues from the embryonic cerebrospinal fluid (eCSF) helping to maintain cell shape and to influence several key functions of aRG such as proliferation and differentiation. Centrosomes, major microtubule organising centres, are crucial for cilia formation. In this review, we focus on how primary cilia influence aRG function during cortical development and pathologies which may arise due to defects in this structure. Reporting and cataloguing a number of ciliary mutant models, we discuss the importance of primary cilia for aRG function and cortical development.