bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of the Ransomware Dataset Age on the Detection Accuracy of Machine Learning Models
Authors: Yaseen Qussai M.
Published: March 2023

Several supervised machine learning models have been proposed and used to detect Android ransomware. These models were trained using different datasets from different sources. However, the age of the ransomware datasets was not considered when training and testing these models. Therefore, the detection accuracy for those models is inaccurate since they learned using features from specific ransomware, old or new ransomware, and they did not learn using diverse ransomware features from different ages. This paper sheds light on the importance of considering the age of ransomware datasets and its effects on the detection accuracy of supervised machine learning models. This proves that supervised machine learning models trained using new ransomware dataset are inefficient in detecting old types of ransomware and vice versa. Moreover, this paper collected a large and diverse dataset of ransomware applications that comprises new and old ransomware developed during the period 2008–2020. Furthermore, the paper proposes a supervised machine learning model that is trained and tested using the diverse dataset. The experiments show that the proposed model is efficient in detecting Android ransomware regardless of its age by achieving an accuracy of approximately 97.48%. Moreover, the results shows that the proposed model outperforms the state-of-the-art approaches considered in this work.
Effectiveness of Artificially Synthesized Granitic Residual Soil-Supported Nano Zero-Valent Iron (Gr-nZVI) as Effective Heavy Metal Contaminant Adsorbent
Authors: Zarime Nur ‘Aishah , Solemon Badariah , Wan Yaacob Wan Zuhairi , Jamil Habibah , Che Omar Rohayu , Oyekanmi Adeleke Abdulrahman
Published: March 2023

Supported nano zero-valent iron is receiving great attention nowadays due to its effectiveness in treating heavy metal pollutants. Therefore, this study aimed to investigate the effectiveness of granitic residual soil-supported nano zero-valent iron (Gr-nZVI) for the removal of the heavy metals Pb2+, Cu2+, Co2+, Cd2+ Ni2+ and Zn2+ in mixture solutions under different experimental conditions of batch equilibrium tests. In this study, Gr-nZVI was successfully synthesized by using the chemical reduction of Ferric Chloride Hexahydrate (FeCl3.6H2O) and Sodium Borohydride (NaBH4). The physical and chemical properties, morphology and mineralogy of all adsorbents were characterized by the Braeuer–Emmett–Teller (BET) method, cation exchange capacity (CEC), X-ray fluorescence (XRF), scanning electron microscopy (SEM), field-emission scanning electron microscopy (FESEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). Isotherm, kinetic and diffusion model analyses were conducted to fit the experimental data. The results show rapid adsorption within 5 min in the initial adsorption stage for Pb2+ on nZVI (qe.Pb = 17.89 mg/g) and Gr-nZVI (qe.Pb = 15.29 mg/g). nZVI and Gr-nZVI also showed no significant effects on pH and temperature, serving as a good example of an energy-efficient process. The isotherm data fitted better to the Langmuir model and the pseudo-second-order kinetic model for the adsorption of all of the heavy metals. The diffusion models revealed that adsorption was not the only rate-limiting step. In this study, nZVI compared to Gr-nZVI and Gr demonstrated superior adsorption capacity for the heavy metal adsorption selectivity. Hence, these materials can be utilized as alternative energy-efficient adsorbents for the adsorption of metal ions from wastewater.
“The Ark of Rights”: Development of a Board Game to Empower Older Adults Regarding Their Rights
Authors: Fernandes Carla Sílvia , Neto Camila , Silva Catarina , Dionísio Sara , Oliveira Susana , Amorim Isabel , Matos Alice Delerue , Martins Maria Manuela
Published: March 2023

There is an urgent need to ensure the rights of older adults. In particular, there is a lack of awareness of human rights by older adults themselves, for which intervention strategies should be developed. Due to the need for intervention at this level, a board game was created to empower older adults regarding their rights using a dynamic and interactive method. This article aims to describe the development stages of the board game “The Ark of Rights”® up to its pilot study. Its development followed three stages: A first phase to review the scientific literature and benchmarks on the rights of older persons, a second phase to define the game design and collect statements from older people for the game, and a third phase to test the game. The European Portuguese Validation of the System Usability Scale (SUS) was used to assess the latter phase. Approximately 200 older people contributed to the game’s contents (second phase), and 74 participated and positively evaluated the game’s usability and their satisfaction with its use (third phase). In summary, the game “The Ark of Rights” revealed itself to be a resource for empowering older adults regarding their rights. It also enables the identification of possible human rights violations among older adults and subsequent intervention.
Effects of Radiofrequency Diathermy Plus Therapeutic Exercises on Pain and Functionality of Patients with Patellofemoral Pain Syndrome: A Randomized Controlled Trial
Authors: Albornoz-Cabello Manuel , Ibáñez-Vera Alfonso Javier , Barrios-Quinta Cristo Jesús , Lara-Palomo Inmaculada Carmen , Cardero-Durán María de los Ángeles , Espejo-Antúnez Luis
Published: March 2023

Although consensus has been reached about the use of therapeutic exercise in patellofemoral pain syndrome, several techniques used worldwide such as radiofrequency diathermy could be useful as complementary therapy. The objective of this randomized controlled trial was to compare the effects of adding radiofrequency diathermy to therapeutic exercises in patients with patellofemoral pain syndrome. Fifty-six participants were randomly assigned either to radiofrequency diathermy plus therapeutic exercises group (n = 29) or therapeutic exercises group (n = 27). Both groups received the same therapeutic exercises, and the diathermy group additionally received monopolar dielectric diathermy for three weeks (5–3–2 weekly sessions). Data related to intensity of pain, probability of neuropathic pain, functionality, and range of movement of the knee were measured at baseline and three weeks after the intervention. Comparing pre-treatment and values obtained at the third week, significant improvements were found in intensity of pain, neuropathic pain, functionality, and range of motion in both groups (p < 0.05). The diathermy plus exercises group had significantly better intensity of pain than the control group at the end of the three weeks (p < 0.01). The addition of diathermy by emission of radiofrequency to the therapeutic knee exercise protocol is more effective than a therapeutic exercise protocol alone in the relief of intensity of pain in patients with patellofemoral pain in the immediate post-treatment follow-up compared with baseline scores.
Association between Systemic Factors and Vitreous Fluid Cytokines in Proliferative Diabetic Retinopathy
Authors: Sato Tomohito , Okazawa Rina , Nagura Koichi , Someya Hideaki , Nishio Yoshiaki , Enoki Toshio , Ito Masataka , Takeuchi Masaru
Published: March 2023

Proliferative diabetic retinopathy (PDR) is a vision-threatening complication of diabetes mellitus (DM). Systemic and intraocular factors are intricately related to PDR, and vitreous fluid (VF) cytokines are representative intraocular biomarkers. However, the associations between systemic factors and VF cytokines and their influence on PDR pathology are unclear. This study aimed to examine the correlation between systemic factors and VF cytokines and analyze their contributions to the pathology of PDR using multivariate analyses. We conducted a retrospective observational study on 26 PDR eyes of 25 patients with type 2 DM, and 30 eyes of 30 patients with idiopathic macular hole or epiretinal membrane as controls. Fifteen systemic and laboratory tests including blood pressure (BP) and body mass index (BMI), and 27 cytokines in VF were analyzed. BP and BMI correlated positively with VF levels of IL-6 and IP-10 in PDR patients, while no significant correlation was found between systemic factors and VF cytokines in controls. MCP-1 and VEGF-A in VF separately clustered with different systemic factors in controls, but these cytokines lost the property similarity with systemic factors and acquired property similarity with each other in PDR. Systemic factors contributed to only 10.4%, whereas VF cytokines contributed to 42.3% out of 52.7% variance of the whole PDR dataset. Our results suggest that intraocular factors play a major role in the pathology of PDR, whereas systemic factors may have limited effects, and that BP and BMI control in PDR could be useful interventions to improve intraocular immune condition.
Macular Anatomy Differs in Dyslexic Subjects
Authors: Garcia-Medina Jose Javier , Bascuñana-Mas Nieves , Sobrado-Calvo Paloma , Gomez-Molina Celia , Rubio-Velazquez Elena , De-Paco-Matallana Maravillas , Zanon-Moreno Vicente , Pinazo-Duran Maria Dolores , Del-Rio-Vellosillo Monica
Published: March 2023

The macula, as the central part of the retina, plays an important role in the reading process. However, its morphology has not been previously studied in the context of dyslexia. In this research, we compared the thickness of the fovea, parafovea and perifovea between dyslexic subjects and normal controls, in 11 retinal segmentations obtained by optical coherence tomography (OCT). With this aim, we considered the nine sectors of the Early Treatment Diabetic Retinopathy Study (ETDRS) grid and also summarized data from sectors into inner ring subfield (parafovea) and outer ring subfield (perifovea). The thickness in all the four parafoveal sectors was significantly thicker in the complete retina, inner retina and middle retina of both eyes in the dyslexic group, as well as other macular sectors (fovea and perifovea) in the inner nuclear layer (INL), inner plexiform layer (IPL), IPL + INL and outer plexiform layer + outer nuclear layer (OPL + ONL). Additionally, the inner ring subfield (parafovea), but not the outer ring subfield (perifovea), was thicker in the complete retina, inner retina, middle retina (INL + OPL + ONL), OPL + ONL, IPL + INL and INL in the dyslexic group for both eyes. In contrast, no differences were found between the groups in any of the sectors or subfields of the outer retina, retinal nerve fiber layer, ganglion cell layer or ganglion cell complex in any eye. Thus, we conclude from this exploratory research that the macular morphology differs between dyslexic and normal control subjects, as measured by OCT, especially in the parafovea at middle retinal segmentations.
Endometrial Cytology in Diagnosis of Endometrial Cancer: A Systematic Review and Meta-Analysis of Diagnostic Accuracy
Authors: Wang Ting , Jiang Ruoan , Yao Yingsha , Wang Yaping , Liu Wu , Qian Linhua , Li Juanqing , Weimer Joerg , Huang Xiufeng
Published: March 2023

Background: Because the incidence of endometrial cancer has been increasing every year, it is important to identify an effective screening method for it. The endometrial cytology test (ECT) is considered to be the more acceptable technique compared to invasive endometrial sampling. Methods: The study followed the Priority Reporting Project for Systematic Evaluation and Meta-Analysis (PRISMA-DTA) protocol. This systematic rating searched EMBASE and Web of Science databases for studies on ECT for endometrial cancer from the databases’ dates of inception to 30 September 2022. All literature screening and data extraction were performed by two researchers, while the methodological quality of the included studies was assessed against defined inclusion criteria. And a third researcher resolves the disagreements. Results: Twenty-six studies were eventually included in this final analysis. Meta-analysis results showed that the diagnostic accuracy characteristics of ECT for endometrial cancer were as follows: combined sensitivity = 0.84 [95% confidence interval (CI) (0.83–0.86)], combined specificity = 0.98 [95% CI (0.98–0.98)], combined positive likelihood ratio = 34.65 [95% CI (20.90–57.45)], combined negative likelihood ratio = 0.21 [95% CI (0.15–0.30)], and area under the summary receiver operating characteristic curve = 0.9673. Conclusions: ECT had the ability to detect endometrial cancer with strong specificity, although some studies have demonstrated significant differences in sensitivity.
Non-Traditional Non-Immunological Risk Factors for Kidney Allograft Loss—Opinion
Authors: Andrian Titus , Siriteanu Lucian , Covic Andreea Simona , Ipate Cristina Alexandra , Miron Adelina , Morosanu Corneliu , Caruntu Irina-Draga , Covic Adrian
Published: March 2023

Rates of late allograft loss have improved slowly in the last decades. Well described traditional risk factors that influence allograft survival include cardiovascular events, rejection, infections and post-transplant neoplasia. Here, we critically evaluate the influence of several non-immunological, non-traditional risk factors and describe their impact on allograft survival and cardiovascular health of kidney transplant recipients. We assessed the following risk factors: arterial stiffness, persistent arteriovenous access, mineral bone disease, immunosuppressive drugs residual levels variability, hypomagnesemia, glomerular pathological alterations not included in Banff criteria, persistent inflammation and metabolic acidosis.
Significant Interactions between Adipokines and Vitamin D Combined with the Estimated Glomerular Filtration Rate: A Geriatric Case Study
Authors: Biercewicz Monika , Kwiatkowska Katarzyna , Kędziora-Kornatowska Kornelia , Krintus Magdalena , Ślusarz Robert , Ruszkowska-Ciastek Barbara
Published: March 2023

Vitamin D deficiency is an important issue in the worldwide population, especially in older people. According to the World Health Organization data, in 2030, 1 in 6 people in the world will be 60 years old or older. The main storage site for vitamin D is adipose tissue. Further, 25(OH)D regulates the expression of adipogenic genes and apoptosis of adipocytes and directly influences the secretion of the appetite-regulating hormone—leptin. Thus, we investigated the impact of the serum concentrations of leptin, adiponectin, omentin, ghrelin, visfatin, and biochemical parameters on vitamin D and estimated glomerular filtration rate (eGFR) in geriatric females. Our studies indicate that the leptin, visfatin and ghrelin are linked with vitamin D concentration and the eGFR rate in the geriatric females. (1) Background: Vitamin D deficiency is common in older people, and researchers are looking for a link between vitamin D deficiency and the occurrence of diseases in advanced age. The study aimed to evaluate the association between serum 25(OH)D levels and clinical variables in older females. (2) Methods: We investigated the impact of the serum concentrations of leptin, adiponectin, omentin, ghrelin, visfatin, and biochemical parameters on vitamin D and estimated the glomerular filtration rate (eGFR) in 74 geriatric females. (3) Results: We observed a significantly higher concentration of creatinine and visfatin in the G2 stage (eGFR = 60–89 mL/min./1.73 m2). We performed an additional analysis to exclude the effect of vitamin D supplementation and obtained a significantly higher vitamin D concentration in the G2 stage. We found significantly lower vitamin D concentrations in older people. In addition, in a person with low levels of vitamin D, we observed significantly lower levels of albumin and ghrelin. Older patients (80 to 89 years old) had significantly lower levels of vitamin D, albumin, insulin, HOMA-IR, and ghrelin than younger patients (60 to 69 years old). Spearman’s correlations performed to examine the relationship between clinical variables seemed to confirm previous results. According to ROC curve analysis, leptin concentration was the strongest predictor of vitamin D fluctuations (the area under the curve, AUC = 0.685; with 79.5% sensitivity and 51.4% specificity; p = 0.0291). However, visfatin reached the most accurate AUCROC = 0.651 with 84.2% sensitivity and 49.1% specificity for predicting effects on eGFR. (4) Conclusions: The results suggest that serum levels of leptin, visfatin, and ghrelin are linked with vitamin D concentration and the eGFR rate in the population of geriatric females.
Digital Health Technologies for Post-Discharge Care after Heart Failure Hospitalisation to Relieve Symptoms and Improve Clinical Outcomes
Authors: Krzesiński Paweł
Published: March 2023

The prevention of recurrent heart failure (HF) hospitalisations is of particular importance, as each such successive event may increase the risk of death. Effective care planning during the vulnerable phase after discharge is crucial for symptom control and improving patient prognosis. Many clinical trials have focused on telemedicine interventions in HF, with varying effects on the primary endpoints. However, the evidence of the effectiveness of telemedicine solutions in cardiology is growing. The scope of this review is to present complementary telemedicine modalities that can support outpatient care of patients recently hospitalised due to worsening HF. Remote disease management models, such as video (tele) consultations, structured telephone support, and remote monitoring of vital signs, were presented as core components of telecare. Invasive and non-invasive monitoring of volume status was described as an important step forward to prevent congestion—the main cause of clinical decompensation. The idea of virtual wards, combining these facilities with in-person visits, strengthens the opportunity for education and enhancement to promote more intensive self-care. Electronic platforms provide coordination of tasks within multidisciplinary teams and structured data that can be effectively used to develop predictive algorithms based on advanced digital science, such as artificial intelligence. The rapid progress in informatics, telematics, and device technologies provides a wide range of possibilities for further development in this area. However, there are still existing gaps regarding the use of telemedicine solutions in HF patients, and future randomised telemedicine trials and real-life registries are still definitely needed.
Rapid Oral Health Deterioration in Older People—A Narrative Review from a Socio-Economic Perspective
Authors: Slack-Smith Linda , Arena Gina , See Lydia
Published: March 2023

Poor oral health is a common morbidity in old age with older adults less likely to attend dental care and more likely to have dental disease; this situation is exacerbated by older adults retaining more teeth often with complex restorations. Evidence suggests that some older adults experience rapid oral health deterioration (ROHD). While more clinical and population level evidence is needed, current evidence suggests upstream changes addressing disadvantage through the social determinants of health (SDH) may impact broader disorders such as ROHD, often occurring as older adults become dependent. The aim of this paper is to conduct a narrative review to explore the social determinants of ROHD in older adults. The social determinants of health are important in understanding oral health including ROHD. This includes the important influence of the economic determinants. We explored the SDH as relevant to oral health and ROHD including using a framework based on that of the Fisher-Owens conceptual model (for children) but adapted for older adults. Better understanding of these relationships is likely to assist in future prevention and care.
Adipogenesis-Related Metabolic Condition Affects Shear-Stressed Endothelial Cells Activity Responding to Titanium
Authors: Pinto Thaís Silva , Gomes Anderson Moreira , de Morais Paula Bertin , Zambuzzi Willian F.
Published: March 2023

Purpose: Obesity has increased around the world. Obese individuals need to be better assisted, with special attention given to dental and medical specialties. Among obesity-related complications, the osseointegration of dental implants has raised concerns. This mechanism depends on healthy angiogenesis surrounding the implanted devices. As an experimental analysis able to mimic this issue is currently lacking, we address this issue by proposing an in vitro high-adipogenesis model using differentiated adipocytes to further investigate their endocrine and synergic effect in endothelial cells responding to titanium. Materials and methods: Firstly, adipocytes (3T3-L1 cell line) were differentiated under two experimental conditions: Ctrl (normal glucose concentration) and High-Glucose Medium (50 mM of glucose), which was validated using Oil Red O Staining and inflammatory markers gene expression by qPCR. Further, the adipocyte-conditioned medium was enriched by two types of titanium-related surfaces: Dual Acid-Etching (DAE) and Nano-Hydroxyapatite blasted surfaces (nHA) for up to 24 h. Finally, the endothelial cells (ECs) were exposed in those conditioned media under shear stress mimicking blood flow. Important genes related to angiogenesis were then evaluated by using RT-qPCR and Western blot. Results: Firstly, the high-adipogenicity model using 3T3-L1 adipocytes was validated presenting an increase in the oxidative stress markers, concomitantly with an increase in intracellular fat droplets, pro-inflammatory-related gene expressions, and also the ECM remodeling, as well as modulating mitogen-activated protein kinases (MAPKs). Additionally, Src was evaluated by Western blot, and its modulation can be related to EC survival signaling. Conclusion: Our study provides an experimental model of high adipogenesis in vitro by establishing a pro-inflammatory environment and intracellular fat droplets. Additionally, the efficacy of this model to evaluate the EC response to titanium-enriched mediums under adipogenicity-related metabolic conditions was analyzed, revealing significant interference with EC performance. Altogether, these data gather valuable findings on understanding the reasons for the higher percentage of implant failures in obese individuals.
Intermittent Exposure to a 16 Hz Extremely Low Frequency Pulsed Electromagnetic Field Promotes Osteogenesis In Vitro through Activating Piezo 1-Induced Ca2+ Influx in Osteoprogenitor Cells
Authors: Chen Yangmengfan , Braun Benedikt J. , Menger Maximilian M. , Ronniger Michael , Falldorf Karsten , Histing Tina , Nussler Andreas K. , Ehnert Sabrina
Published: March 2023

Exposure to extremely low frequency pulsed electromagnetic fields (ELF-PEMF) is supposed to simulate local EMF generated during mechanical stimulation of bone and may therefore be used to improve bone regeneration. This study aimed at optimizing the exposure strategy and investigating the underlying mechanisms of a 16 Hz ELF-PEMF, previously reported to boost osteoblast function. Comparing influences of daily continuous (30 min every 24 h) and intermittent (10 min every 8 h) exposure to the 16 Hz ELF-PEMF on osteoprogenitor cells revealed that the intermittent exposure strategy enhanced the 16 Hz ELF-PEMF effects regarding cell numbers and osteogenic function. Gene expression of piezo 1 and related Ca2+ influx were significantly increased in SCP-1 cells with the daily intermittent exposure. Pharmacological inhibition of piezo 1 with Dooku 1 largely abolished the positive effect of the 16 Hz ELF-PEMF exposure on osteogenic maturation of SCP-1 cells. In summary, the intermittent exposure strategy enhanced the positive effects of 16 Hz continuous ELF-PEMF exposure in terms of cell viability and osteogenesis. This effect was shown to be mediated by an increased expression of piezo 1 and related Ca2+ influx. Thus, the intermittent exposure strategy is a promising way to further optimize the therapeutic effects of the 16 Hz ELF-PEMF regarding fracture healing or osteoporosis.
6D Object Localization in Car-Assembly Industrial Environment
Authors: Papadaki Alexandra , Pateraki Maria
Published: March 2023

In this work, a visual object detection and localization workflow integrated into a robotic platform is presented for the 6D pose estimation of objects with challenging characteristics in terms of weak texture, surface properties and symmetries. The workflow is used as part of a module for object pose estimation deployed to a mobile robotic platform that exploits the Robot Operating System (ROS) as middleware. The objects of interest aim to support robot grasping in the context of human–robot collaboration during car door assembly in industrial manufacturing environments. In addition to the special object properties, these environments are inherently characterised by cluttered background and unfavorable illumination conditions. For the purpose of this specific application, two different datasets were collected and annotated for training a learning-based method that extracts the object pose from a single frame. The first dataset was acquired in controlled laboratory conditions and the second in the actual indoor industrial environment. Different models were trained based on the individual datasets and a combination of them were further evaluated in a number of test sequences from the actual industrial environment. The qualitative and quantitative results demonstrate the potential of the presented method in relevant industrial applications.
Numerical Investigation of Step Size Effect on Formability of 2024-T3 Aluminum in Incremental Forming
Authors: Grimm Tyler J. , Colombini Filipe , Ragai Ihab
Published: March 2023

Incremental forming (IF) is an advanced manufacturing process in which a forming tool locally deforms sheet material into a desired geometry through successive passes at incremental depths. An inherent benefit to the IF process is its formability improvement over conventional stamping; however, further enhancements will enable the forming of increasingly complex geometries. To progress the IF process towards heavy industrial use, the modeling of such processes must be further developed. Single point incremental forming (SPIF) of AA2024-T3 was modeled herein utilizing explicit formulations. The model geometry featured a nominally rectangular-shaped clamping region. A friction factor was experimentally determined and utilized within the model, which is a novel addition to this work. Formability was determined and forming limit diagrams were composed. It was found that the present model shows greater formability and underestimates plastic strain compared to experimental testing. The generation of forming limit diagrams for this material processed by IF is also a novel addition to this field.
Wave Overtopping at Sea Dikes on Shallow Foreshores: A Review, an Evaluation, and Remaining Challenges
Authors: Tarakcioglu Gulizar Ozyurt , Kisacik Dogan , Gruwez Vincent , Troch Peter
Published: March 2023

Wave overtopping is a critical parameter in the design of coastal defense structures. Nowadays, several empirical formulations based on small-scale experiments are available in the literature to predict the mean overtopping discharge at dikes on shallow foreshores. Although the accuracy of the predictions has improved due to each approach’s contributions, the formulations’ performance depends on their range of applicability. In engineering applications, it is important to know the performance and limitations of the different formulas. This work presents a new experimental dataset focused on very shallow and extremely shallow foreshore conditions for a range of foreshore slopes (i.e., 1/20, 1/35, 1/50, and 1/80) and relative water depths. The recent developments in wave overtopping research on very shallow and extremely shallow foreshore conditions have been reviewed using this dataset to reflect the existing uncertainties and challenges in the wave-overtopping literature. We find that predicting wave overtopping for extremely shallow foreshore conditions still requires improvement. Additional research is needed to understand the (residual) influence on the wave overtopping of the foreshore slope and relative magnitude of the infragravity wave height to the sea-swell wave height at the dike toe, especially for extremely shallow foreshore conditions. The variation in performance of the formulas for different foreshore slopes is demonstrated. Finally, some of the remaining uncertainties that need further exploration are discussed.
Analysis of Water Resonance in the Inner Domain of a Large Fixed Floating Tourist Platform Based on a 3D Non-Hydrostatic Model
Authors: Liu Zhiqiang , Mao Yanjun , Ding Jun , Tian Chao , Wang Siyu , Ai Congfang , Ma Xiaozhou , Dong Guohai
Published: March 2023

In order to meet the needs of marine tourism development, this paper analyzes the water resonance in the inner domain of a fixed floating tourist platform based on a 3D non-hydrostatic model. The tourist platform has a complex structure that is prone to the resonance of the water in the inner domain under wave actions. The water’s motion response in the platform’s inner domain under wave action is simulated numerically using numerical simulation methods. The non-hydrostatic model used in this paper is based on a semi-implicit method to solve the incompressible Navier–Stokes equations (NSE) and combines the immersed boundary method with the global continuity equations in the pressure zone (the flow zone under the structure) to approach the problem. In this paper, firstly, the resonant frequencies and resonant amplitudes of the water inside the box-shaped ship are calculated and compared with the experimental data to demonstrate the non-hydrostatic model’s accuracy in calculating the resonance problem of the water in the inner domain of a fixed floating platform. Secondly, the water’s resonant frequencies and resonant modes in the inner domain are calculated by numerical modeling of the floating tourist platform, including rare high-order resonance modes. We found that there are six resonance modes with complex shapes in the water of the inner domain of the tourist platform. Furthermore, the effects of the wall thickness, chord distance and draft of the annular tourist platform on the resonant amplitude values and modes of the water domain within the platform are analyzed in depth.
Safe Design of a Hydrogen-Powered Ship: CFD Simulation on Hydrogen Leakage in the Fuel Cell Room
Authors: Guan Wenfeng , Chen Ju , Chen Lijian , Cao Jiaolong , Fan Hongjun
Published: March 2023

Adopting proton exchange membrane fuel cells fuelled by hydrogen presents a promising solution for the shipping industry’s deep decarbonisation. However, the potential safety risks associated with hydrogen leakage pose a significant challenge to the development of hydrogen-powered ships. This study examines the safe design principles and leakage risks of the hydrogen gas supply system of China’s first newbuilt hydrogen-powered ship. This study utilises the computational fluid dynamics tool FLACS to analyse the hydrogen dispersion behaviour and concentration distributions in the hydrogen fuel cell room based on the ship’s parameters. This study predicts the flammable gas cloud and time points when gas monitoring points first reach the hydrogen volume concentrations of 0.8% and 1.6% in various leakage scenarios, including four different diameters (1, 3, 5, and 10 mm) and five different directions. This study’s findings indicate that smaller hydrogen pipeline diameters contribute to increased hydrogen safety. Specifically, in the hydrogen fuel cell room, a single-point leakage in a hydrogen pipeline with an inner diameter not exceeding 3 mm eliminates the possibility of flammable gas cloud explosions. Following a 10 mm leakage diameter, the hydrogen concentration in nearly all room positions reaches 4.0% within 6 s of leakage. While the leakage diameter does not impact the location of the monitoring point that first activates the hydrogen leak alarm and triggers an emergency hydrogen supply shutdown, the presence of obstructions near hydrogen detectors and the leakage direction can affect it. These insights provide guidance on the optimal locations for hydrogen detectors in the fuel cell room and the pipeline diameters on hydrogen gas supply systems, which can facilitate the safe design of hydrogen-powered ships.
Mycoviruses in Fungi: Carcinogenesis of Fungal Agents May Not Always Be Mycotoxin Related
Authors: Tebbi Cameron K.
Published: March 2023

Certain viruses have been found to induce diverse biological pathways to carcinogenesis, evidenced by the presence of viral gene products in some tumors. Despite the fact that many fungal agents contain mycoviruses, until recently, their possible direct effects on human health, including carcinogenesis and leukemogenesis, had not been explored. In this regard, most studies of fungal agents have rightly concentrated on their mycotoxin formation and effects. Recently, the direct role of yeasts and fungi in the etiology of cancers, including leukemia, have been investigated. While greater attention has been placed on the carcinogenic effects of Candida, the role of filamentous fungi in carcinogenesis has also been explored. Recent findings from studies using the enzyme-linked immunosorbent assay (ELISA) technique indicate that the plasma of patients with acute lymphoblastic leukemia (ALL) uniformly contains antibodies for a certain mycovirus-containing Aspergillus flavus, while controls are negative. The exposure of mononuclear leukocytes from patients with ALL in full remission, and long-term survivors, to the product of this organism was reported to result in the re-development of typical genetics and cell surface phenotypes characteristic of active ALL. Mycoviruses are known to be able to significantly alter the biological characteristics and functions of their host. The possible carcinogenic and leukemogenic role of mycoviruses, with and without their host, needs to be further investigated.
Chemistry in Fungal Bioluminescence: Theoretical Studies on Biosynthesis of Luciferin from Caffeic Acid and Regeneration of Caffeic Acid from Oxidized Luciferin
Authors: Liu Xiayu , Wang Mingyu , Liu Yajun
Published: March 2023

Fungal bioluminescence is widely distributed in the terrestrial environment. At a specific stage of growth, luminescent fungi shine green light at the fruiting body or mycelium. From the viewpoint of chemistry, fungal bioluminescence involves an in vivo cycle of caffeic acid. The complete cycle is composed of three stages: biosynthesis of luciferin from caffeic acid, luminescence process from luciferin to oxidized luciferin, and regeneration of caffeic acid from oxidized luciferin. Experimental studies roughly proposed this cycle but not the detailed reaction process and mechanism. Our previous theoretical study clearly described the mechanism of the middle stage. The present article attempts to describe the reaction processes and mechanisms of the other two stages by theoretical calculations. A complete theoretical study on the chemistry in the entire process of fungal bioluminescence is helpful to deeply understand fungal bioluminescence.
Transcriptomic and Metabolomic Profiles Provide Insights into the Red-Stipe Symptom of Morel Fruiting Bodies
Authors: Yang Chi , Jiang Xiaoling , Ma Lu , Xiao Donglai , Liu Xiaoyu , Ying Zhenghe , Li Yaru , Lin Yanquan
Published: March 2023

The cultivation of true morels (Morchella spp., Morchellaceae, Ascomycota) has rapidly expanded in recent years, especially in China. Red stipe is a symptom wherein the stipe of morel fruiting bodies becomes red–gray, resulting in the gradual death of the affected fruiting bodies. The impact of red-stipe symptom occurrence on the development and nutritional quality of morel fruiting bodies remains unclear. Herein, morel ascocarps with the red-stipe symptom (R) and normal (N), artificially cultivated in the Fujian Province of China, were selected for the transcriptome and metabolome analysis to study the physiological and biochemical responses of morel fruiting bodies to the red-stipe symptom. Transcriptome data revealed several differentially expressed genes between the R and N groups significantly enriched in the tyrosine, riboflavin, and glycerophospholipid metabolism pathways. Similarly, the differentially accumulated metabolites were mainly assigned to metabolic pathways, including tyrosine, the biosynthesis of plant secondary metabolites, and the biosynthesis of amino acids. Moreover, the transcriptome and metabolome data combination revealed that tyrosine metabolism was the most enriched pathway, which was followed by ATP-binding cassette (ABC) transport, alanine, aspartate, and glutamate metabolism. Overall, the integration of transcriptomic and metabolomic data of M. sextelata affected by red-stipe symptoms identified several important genes, metabolites, and pathways. These findings further improve our understanding of the mechanisms underlying the red-stipe symptom development of M. sextelata and provide new insights into how to optimize its cultivation methods.
Characterization of Aspartic Proteases from Paracoccidioides brasiliensis and Their Role in Fungal Thermo-Dimorphism
Authors: Silva Rafael de Souza , Segura Wilson Dias , Oliveira Reinaldo Souza , Xander Patricia , Batista Wagner Luiz
Published: March 2023

Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin America and is caused by fungi from the Paracoccidioides genus. The infection begins after inhalation of the fungal propagules and their thermo-dimorphic shift to yeast form. Proteases play an important role in the host invasion process and immune modulation in many pathogenic microorganisms. Aspartyl proteases are virulence factors in many human fungal pathogens that play an important role in the host invasion process morphogenesis, cellular function, immunity, and nutrition. In the present study, we characterized the modulation of acid proteases from Paracoccidioides brasiliensis. We detected four aspartyl proteases in P. brasiliensis with high homology to aspartic protease from Saccharomyces cerevisiae Pep4. Furthermore, we demonstrated that Pepstatin A can inhibit dimorphic switching (mycelium→yeast) in P. brasiliensis. In addition, these genes were modulated during thermo-dimorphism (M→Y transition) in the presence or absence of carbon and nitrogen sources and during growth at pH 4 during 24 and 48 h. We also observed that P. brasiliensis increase the secretion of aspartic proteases when cultivated at pH 4, and these acid proteases cleave BSA, collagen, and hemoglobin. These data suggest that aspartyl proteases are modulated by environmental conditions and during fungal thermo-dimorphism. Thus, this work brings new possibilities for studying the role of aspartyl proteases in the host–pathogen relationship and P. brasiliensis biology.
Baseline Analysis of Endophytic Fungal Associates of Solenopsis invicta Buren from Mounds across Five Counties of Guangdong Province, China
Authors: Bamisile Bamisope Steve , Siddiqui Junaid Ali , Nie Lei , Idrees Atif , Aguila Luis Carlos Ramos , Jia Chunsheng , Xu Yijuan
Published: March 2023

Red imported fire ants mounds have been suggested as a potential reservoir for beneficial entomopathogenic fungal species that are vital for more complex roles in the ecosystem aside from infecting the insects. In the current study, the assemblage of fungal symbionts of the red imported fire ants (RIFA) were obtained across five cities in Guangdong Province, China. The sampling areas were selected because of high occurrence of fire ants mounds in the regions. Mound soils, plant debris within mounds, and ants were collected from three sampling locations in each city for potential isolation of entomopathogenic fungal associates of RIFA. All samples were collected during the spring of 2021. Following successful isolation from substrates, the patterns of fungal species composition, and richness were evaluated. In total, 843 isolates were recovered, and based on their phenotypic distinctiveness and molecular characterization based on DNA sequences of multiple loci including the ITS, SSU, and LSU regions, 46 fungal taxa were obtained, including 12 that were unidentified. Species richness and abundance was highest in the mound soils, while the lowest value was recorded from the ant body. As per the different locations, the highest abundance level was recorded in Zhuhai, where 15 fungal taxa were cultivated. The most common taxa across all substrates and locations was Talaromyces diversus. A baseline analysis of the fungal community composition of RIFA would better our understanding on the interactions between these social ants and their associated microbial organisms, and this knowledge in turn would be important for the successful management of the RIFA.
Anthropometric Features and Third-Fourth Degree Perineal Tears
Authors: Fruscalzo Arrigo , Novak Alice , Somma Camilla , Xholli Anjeza , Michelerio Virginia , Prefumo Federico , Londero Ambrogio , Cagnacci Angelo
Published: March 2023

The main objective of this study was to evaluate the association between maternal and fetal anthropometric characteristics and third- and fourth-degree perineal tears. This retrospective cohort study considered all consecutive pregnancies from 2011 to 2017 at a single Institution. The inclusion criteria were: singletons who delivered vaginally during the study period, the presence of information on maternal pre-pregnancy weight, maternal height, and weight of the newborn. The feto-maternal body-mass index (BMI) was calculated as neonatal weight in kg on maternal height in squared meters (kg/m2). In total, 5397 singleton-term pregnancies were included; the prevalence of third-fourth-degree perineal tears was 0.47%. The most predictive factors were: nulliparity, feto-maternal BMI, neonatal weight, gestational age at delivery, and neonatal head circumference. After adjustment in multivariate analysis, the only independent predictors were nulliparity and fetomaternal BMI. The AUC of the final multivariate model was 73.54% (95% CI 65.65–81.42). Furthermore, feto-maternal BMI and gestational age had a significant direct correlation. Nulliparity and feto-maternal BMI are the two best predictors for third and fourth-degree perineal tears in our setting. Confirming this association in future research and integrating it into a decision algorithm on delivery timing could reduce obstetric damage to the anal sphincter.
Diagnostic Value of the Combined Measurement of Serum HCY and NRG4 in Type 2 Diabetes Mellitus with Early Complicating Diabetic Nephropathy
Authors: Ding Sheng , Yang Yi , Zheng Yuming , Xu Jinling , Cheng Yangyang , Wei Wei , Yu Fuding , Li Li , Li Menglan , Wang Mengjie , Wang Zhongjing , Xiang Guangda
Published: March 2023

Purpose: This study aimed to investigate the value of combined detection of HCY and NRG4 in the diagnosis of early diabetic kidney disease (DKD) and to explore the association between the ratio of HCY/NRG4 and DKD. Methods: A total of 140 diabetic patients and 43 healthy people were prospectively enrolled. The plasma HCY level, NRG4 level and HCY/NRG4 of them were measured to compare their differences and analyze the correlation with DKD. The independent influencing factors of patients with DKD were screened, and the nomograph of DKD occurrence was constructed. Results: The levels of HCY and HCY/NRG4 in diabetic patients were significantly increased, while the level of NRG4 was significantly decreased (p < 0.01). The AUCs of HCY/NRG4 predicted for DKD were 0.961. HCY/NRG4 and the course of DM were independent risk factors for DKD. A predictive nomograph of DKD was constructed, and decision curve analysis (DCA) showed good clinical application value. HCY/NRG4 was positively correlated with Scr, UACR, TG, UA, BUN, TCHOL and LDL and negatively correlated with eGFR and HDL (p < 0.05). Conclusions: The level of HCY and NRG4 is closely related to the severity of DM, and combined detection of HCY/NRG4 can identify patients with DKD at an early stage.
Diametric Growth of a Forest under Reduced-Impact Logging in the Eastern Region of the Brazilian Amazon
Authors: Flores William Montero , França Isadora , Santos Graciliano Galdino Alves dos , Miranda Izildinha de Souza , Moraes Eric Fabricio Santos , Sánchez Gustavo Hernández , Silva Sandra Dezuite Balieiro Da , Hernández-Ruz Emil José
Published: March 2023

Growth is a component of forest dynamics that encompasses changes in species composition, interactions between species, and stand structure. We evaluated the effect of the presence of lianas, crown shape, and light exposure on the periodic annual increment of trees (PAI) in two phytophysiognomies: the ombrophilous dense forest and the ombrophilous open forest with lianas. This study was conducted in the eastern Brazilian Amazon in 13 permanent monitoring plots of 50 m × 50 m (2500 m2) in the same annual production unit. The area has been subjected to reduced-impact logging from 2014 to 2016. The increment in tree diameter varied significantly between both forests (opens forests: 1.28 cm yr−1; dense forests: 0.82 cm yr−1). Light exposure influenced the increment in tree diameter in the ombrophilous open forest with lianas, while the crown shape and light exposure significantly affected the ombrophilous dense forest. Our study emphasizes the usefulness of the periodic annual increment in exploring the differences between contrasting phytophysiognomies in a single annual production unit in the Brazilian Amazon that is subject to reduced-impact logging.
Mechanisms of Hydride Nucleation, Growth, Reorientation, and Embrittlement in Zirconium: A Review
Authors: Jia Yu-Jie , Han Wei-Zhong
Published: March 2023

Zirconium (Zr) hydrides threaten the reliability of fuel assembly and have repeatedly induced failures in cladding tubes and pressure vessels. Thus, they attract a broad range of research interests. For example, delayed hydride cracking induced a severe fracture and failure in a Zircaloy-2 pressure tube in 1983, causing the emergency shutdown of the Pickering nuclear reactor. Hydride has high hardness and very low toughness, and it tends to aggregate toward cooler or tensile regions, which initiates localized hydride precipitation and results in delayed hydride cracking. Notably, hydride reorientation under tensile stress substantially decreases the fracture toughness and increases the ductile-to-brittle transition temperature of Zr alloys, which reduces the safety of the long-term storage of spent nuclear fuel. Therefore, improving our knowledge of Zr hydrides is useful for effectively controlling hydride embrittlement in fuel assembly. The aim of this review is to reorganize the mechanisms of hydride nucleation and growth behaviors, hydride reorientation under external stress, and hydride-induced embrittlement. We revisit important examples of progress of research in this field and emphasize the key future aspects of research on Zr hydrides.
Refined Dual-Phase-Lag Theory for the 1D Behavior of Skin Tissue under Ramp-Type Heating
Authors: Zenkour Ashraf M. , Saeed Tareq , Aati Amal M.
Published: March 2023

In this article, a mathematical analysis of thermoelastic skin tissue is presented based on a refined dual-phase-lag (DPL) thermal conduction theory that considers accounting for the effect of multiple time derivatives. The thin skin tissue is regarded as having mechanically clamped surfaces that are one-dimensional. Additionally, the skin tissue undergoes ramp-type heating on its outer surface, whereas its inner surface keeps the assessed temperature from vanishing. Some of the previous generalized thermoelasticity theories were obtained from the proposed model. The distributions of temperature, displacement, dilatation, and stress are attained by applying the Laplace transform and its numerical reversal approaches. The outcomes are explicitly illustrated to examine the significant influences on the distributions of the field variables. The refined DPL bioheat conduction model in this study predicts temperature, and the findings revealed that the model is located among the existing generalized thermoelastic theories. These findings offer a more thorough understanding of how skin tissue behaves when exposed to a particular boundary condition temperature distribution.