bluuebunny/crossref_metadata_2025 huggingface dataset:
In Silico Analysis of Triamterene as a Potential Dual Inhibitor of VEGFR-2 and c-Met Receptors
Authors: Lutimba Stuart , Saleem Baraya , Aleem Eiman , Mansour Mohammed A.
Published: December 2024

The vascular endothelial growth factor receptor 2 (VEGFR2) and the hepatocyte growth factor receptor (C-Met) are critical receptors for signaling pathways controlling crucial cellular processes such as cell growth, angiogenesis and tissue regeneration. However, dysregulation of these proteins has been reported in different diseases, particularly cancer, where these proteins promote tumour growth, invasiveness, metastasis and resistance to conventional therapies. The identification of dual inhibitors targeting both VEGFR-2 and c-Met has emerged as a strategic therapeutic approach to overcome the limitations and resistance mechanisms associated with single-target therapies in clinical settings. Through molecular dynamics simulations and comparative docking analysis, we tested the inhibitory potential of 2,016 Food and Drug Administration (FDA)-approved drugs targeting VEGFR-2 and/or c-Met receptors. The results revealed that entacapone and telmisartan are potent and selective inhibitors for c-Met and VEGFR-2, respectively. Interestingly, triamterene was identified as a promising dual inhibitor, demonstrating specific and significant binding affinity to both proteins. Molecular dynamics simulations revealed key interactions between the identified compounds and critical residues in the catalytic domains of both VEGFR-2 (e.g., Lys868, Asp1028, Asp1046) and c-Met (e.g., Asp1204, His1202, Asp1222), providing insights into their mechanism of action. These findings underscore the therapeutic potential of triamterene in targeting multiple signaling pathways involved in cancer progression, metastasis and poor prognosis in patients. Our study provides a foundational framework for the development of novel anticancer compounds able to target multiple pathways in cancer. Further preclinical and clinical investigations are needed to validate the efficacy of these compounds in clinical settings and to test their ability to overcome resistance and improve patient outcome.
Corticosteroids in Pediatric Septic Shock: A Narrative Review
Authors: Rulli Immacolata , Carcione Angelo Mattia , D’Amico Federica , Quartarone Giuseppa , Chimenz Roberto , Gitto Eloisa
Published: December 2024

Objective: A controversial aspect of pediatric septic shock management is corticosteroid therapy. Current guidelines do not recommend its use in forms responsive to fluids and inotropes but leave the decision to physicians in forms refractory to the first steps of therapy. Data Sources: Review of literature from January 2013 to December 2023 from online libraries Pubmed, Medline, Cochrane Library, and Scopus. Study Selection: The keywords “septic shock”, “steroids” and “children” were used. Data Extraction: Of 399 articles, 63 were selected. Data Synthesis: Regarding mortality, although the 2019 Cochrane review supports reduced mortality, benefits on long-term mortality and in patients with CIRCI (critical illness-related corticosteroid insufficiency) are not clear. Yang’s metanalysis and retrospective studies of Nichols and Atkinson show no difference or even an increase in mortality. Regarding severity, the Cochrane review claims that hydrocortisone seems to reduce the length of intensive care hospitalization but influences the duration of ventilatory and inotropic support, and the degree of multi-organ failure appears limited. Further controversies exist on adrenal function evaluation: according to literature, including the Surviving Sepsis Campaign guidelines, basal or stimulated hormonal dosages do not allow the identification of patients who could benefit from hydrocortisone therapy (poor reproducibility). Regarding side effects, muscle weakness, hypernatremia, and hyperglycemia are the most observed. Conclusions: The literature does not give certainties about the efficacy of corticosteroids in pediatric septic shock, as their influence on primary outcomes (mortality and severity) is controversial. A subgroup of patients suffering from secondary adrenal insufficiency could benefit from it, but it remains to be defined how to identify and what protocol to use to treat them.
Dynamics of Dividend Payout in Korean Corporations: A Comprehensive Panel Analysis Across Economic Cycles
Authors: Choi SungSup Brian , Sauka Kudzai
Published: December 2024

This research conducts a meticulous examination of the determinants influencing dividend payout dynamics among firms listed on the Korean Stock Exchange (KSE) from 1995 to 2021, a period characterized by profound economic fluctuations. By leveraging a dynamic panel data model and the Generalized Method of Moments (GMM) for estimation, the study addresses endogeneity concerns while exploring the effects of firm-specific and macroeconomic variables on dividend yields. The investigation delineates three distinct economic phases: normal conditions, financial crises, and the aggregate study period, facilitating a granular understanding of firms’ dividend payout adaptability under varying economic landscapes. Empirical findings underscore the persistence of dividend payments, revealing a variable adjustment speed toward target dividend yields contingent upon the economic context, with an expedited adjustment observed during crises. Crucially, firm profitability emerges as a consistent determinant of dividend yields across all examined periods, whereas the influence of macroeconomic variables is notably more pronounced during periods of economic normalcy. This research elucidates the complex interplay between internal corporate strategies and external economic pressures in shaping dividend policies, thereby enriching the discourse on dividend payout behavior in the context of Korea’s economic evolution from an emerging to a developed market.
Exploring the Functionality of the Krüppel-like Factors in Kidney Development, Metabolism, and Diseases
Authors: Salmon-Cabrales Itzel S. , de la Garza-Kalife David A. , García-González Gabriel , Estrada-Rodríguez Ana E. , Jiménez-Gutiérrez Marco Antonio , Santoyo-Suárez Michelle G. , Rodríguez-Núñez Oscar , Garza-Treviño Elsa N. , Benítez-Chao Diego F. , Padilla-Rivas Gerardo R. , Islas Jose Francisco
Published: December 2024

The kidneys contribute to the overall health of an organism by maintaining systemic homeostasis. This process involves various biological mechanisms, in which the Krüppel-like factors (KLFs), a family of transcription factors, are essential for regulating development, differentiation, proliferation, and cellular apoptosis. They also play a role in the metabolic regulation of essential nutrients, such as glucose and lipids. The dysregulation of these transcription factors is associated with the development of various pathologies, which can ultimately lead to renal fibrosis, severely compromising kidney function. In this context, the present article provides a comprehensive review of the existing literature, offering an enriching analysis of the findings related to the role of KLFs in nephrology, while also highlighting their potential therapeutic role in the treatment of renal diseases.
Dexmedetomidine Improves Learning Functions in Male Rats Modeling Cognitive Impairment by Modulating the BDNF/TrkB/CREB Signaling Pathway
Authors: Saral Sinan , Mercantepe Tolga , Topçu Atilla , Kaya Ali Koray , Öztürk Aykut
Published: December 2024

Dexmedetomidine (DEX) is a selective alpha-2 adrenergic receptor agonist with sedative and anxiolytic properties. Increasing evidence reports that DEX has a neuroprotective effect. In this study, we investigated the potential effects of DEX on learning and memory functions in rats with experimental cognitive impairment. In the study, 21 adult male rats were used. The rats were divided into three groups, namely control, Scopolamine (SCOP) and SCOP + DEX. Cognitive impairment was induced with 1 mg/kg SCOP daily for 21 days. DEX was administered at a dose of 10 µg/kg between days 14 and 21 of the experiment. Following the injections, a spatial memory test was performed with a Morris Water Maze (MWM). At the end of the experiment, the hippocampus was dissected. The brain-derived neurotrophic factor (BDNF), acetylcholine (ACh) and acetylcholinesterase (AChE) levels were determined by ELISA. The tropomyosin receptor kinase B (TrkB) and Cyclic AMP-Response Element-Binding Protein (CREB) levels were measured by immunohistochemistry. DEX treatment improved the learning performance of rats compared to SCOP for 5 days. However, it did not significantly change memory performance. DEX increased the BDNF and ACh levels in the hippocampus while decreasing the AChE levels. Similarly, DEX treatment significantly increased CREB phosphorylation. No significant difference was observed between the TrkB receptor levels of the groups. This study demonstrated that the role of DEX in reducing SCOP-induced cognitive impairment is partially mediated by the increase in BDNF/TrkB/CREB signaling pathway activity.
Immunomodulatory Properties of Multi-Strain Postbiotics on Human CD14+ Monocytes
Authors: Roberts Kyle D. , Ahmed Sadia , San Valentin Erin , Di Martino Luca , McCormick Thomas S. , Ghannoum Mahmoud A.
Published: December 2024

The ability of probiotics, comprising live microbiota, to modulate the composition of intestinal microbiomes has been connected to modulation of the central nervous system (Gut–Brain axis), neuroendocrine system (Gut–Skin axis), and immune response (Gut–Immune axis). Less information is known regarding the ability of postbiotics (cell wall components and secreted metabolites derived from live organisms) to regulate host immunity. In the present study, we tested postbiotics comprising single strains of bacteria and yeast (Lactobacillus acidophilus 16axg, Lacticaseibacillus rhamnosus 18fx, Saccharomyces cerevisiae var. boulardii 16mxg) as well as combinations of multiple strains for their ability to stimulate cytokine production by human CD14+ monocytes. We quantified cytokine gene and protein expression levels in monocytes following stimulation with postbiotics. Both heat-killed L. acidophilus and L. rhamnosus stimulated naïve monocytes without significant differences between them. Heat-killed S. boulardii stimulated less cytokine production compared to postbiotic bacteria at the same concentration. Interestingly, the addition of heat-killed yeast to heat-killed L. acidophilus and L. rhamnosus resulted in an enhancement of immune stimulation. Thus, heat-killed postbiotics have immune-modulating potential, particularly when bacteria and yeast are combined. This approach may hold promise for developing targeted interventions that can be fine-tuned to modulate host immune response with beneficial health impact.
Novel Numerical Method for Studying Water Freezing on Surfaces Texturized by Laser
Authors: Haj Ibrahim Samih , Wejrzanowski Tomasz , Karl Christian W. , Sagvolden Espen , Karwaszewski Jakub , Pilz Monika , Przybyszewski Bartłomiej , Kozera Rafał
Published: December 2024

Within this study, a methodology for the numerical simulation of droplet freezing, including a micrometer texturized pattern, was developed. The finite volume method was then applied to simulate the behavior of water droplets. The procedure was divided into two processes: stabilization and freezing. In the stabilization step, the droplet was dropped onto the material surface and took an equilibrium shape. In the second step, additional energy equation and temperature boundary conditions were applied to perform freezing simulation. Based on the laser-texturized samples of polyurethane-coated metal substrates studied with freezing delay experiments, numerical models were generated, and droplet freezing simulations were performed. Three cases were studied—non-texturized and texturized with respectively linear and triangular patterns. The obtained simulation results of freezing time were compared with experimental measurements to evaluate the proposed methodology. The study revealed that despite the inability to predict accurate freezing delay time, the proposed methodology can be used to compare the freezing delay capabilities for different texturized patterns. Additionally, the proposed model renders it possible to analyze additional aspects of wetting and freezing of the droplet on rough surfaces, which may be helpful in understanding these processes.
Novel and Sustainable Materials for the Separation of Lithium, Rubidium, and Cesium Ions from Aqueous Solutions in Adsorption Processes—A Review
Authors: Kaczorowska Małgorzata A.
Published: December 2024

The growing demand for alkali metals (AMs), such as lithium, cesium, and rubidium, related to their wide application across various industries (e.g., electronics, medicine, aerospace, etc.) and the limited resources of their naturally occurring ores, has led to an increased interest in methods of their recovery from secondary sources (e.g., brines, wastewater, waste leachates). One of the dynamically developing research directions in the field of separation of AMs ions from various aqueous solutions is the search for novel, efficient, and “green” materials that could be used in adsorption processes, also on a larger industrial scale. This review concerns the latest achievements (mainly from 2023 to 2024) in the development of innovative adsorption materials (e.g., ion sieves, aluminum-based adsorbents, mineral adsorbents, composites, resins) for the separation of Li+, Cs+, and Rb+ ions from solutions, with particular emphasis on their most important advantages and limitations, as well as their potential impact on the environment.
Microstructure and Bioactivity of Ca- and Mg-Modified Silicon Oxycarbide-Based Amorphous Ceramics
Authors: Liu Qidong , Chen Hongmei , Wu Xiumei , Yan Junjie , Yang Biaobiao , Shi Chenying , Li Yunping , Yu Shu
Published: December 2024

Silicon oxycarbide (SiOC), Ca- and Mg-modified silicon oxycarbide (SiCaOC and SiMgOC) were synthesized via sol–gel processing with subsequent pyrolysis in an inert gas atmosphere. The physicochemical structures of the materials were characterized by XRD, SEM, FTIR, and 29Si MAS NMR. Biocompatibility and in vitro bioactivity were detected by MTT, cell adhesion assay, and simulated body fluid (SBF) immersion test. Mg and Ca were successfully doped into the network structure of SiOC, and the non-bridging oxygens (NBO) were formed. The hydroxycarbonate apatite (HCA) was formed on the modified SiOC surface after soaking in simulated body fluid (SBF) for 14 days, and the HCA generation rate of SiCaOC was higher than that of SiMgOC. Accompanying the increase of bioactivity, the network connectivity (NC) of the modified SiOC decreased from 6.05 of SiOC to 5.80 of SiCaOC and 5.60 of SiMgOC. However, structural characterization and biological experiments revealed the nonlinear relationship between the biological activity and NC of the modified SiOC materials.
Study on the Synthesis and Electrochemical Properties of Nitrogen-Doped Graphene Quantum Dots
Authors: Wang Yongbo , Wang Yanxiang , Liu Dongming , Feng Yanqiu , Yang Deli , Wu Simeng , Jiang Haotian , Wang Donglong , Bi Shishuai
Published: December 2024

Nitrogen-doped graphene quantum dots (N-GQDs) are widely used in biosensing, catalysis, and energy storage due to their excellent conductivity, high specific surface area, unique quantum size effects, and optical properties. In this paper, we successfully synthesized N-GQDs using a facile hydrothermal approach and investigated the effects of different hydrothermal temperatures and times on the morphology and structure of N-GQDs. The results indicated that the size of N-GQDs gradually increased and they eventually aggregated into graphene fragments with increasing temperature or reaction time. Notably, N-GQDs synthesized at 180 °C for 6 h exhibited the most uniform size, with an average diameter of approximately 3.48 nm, a height of 5–6 graphene layers, as well as favorable fluorescence properties. Moreover, the surface of N-GQDs contained abundant oxygen- and nitrogen-containing functional groups, which could provide numerous active sites for electrode reactions. The assembled electrode exhibited typical pseudocapacitive behavior with exceptional electrochemical performance, achieving a specific capacitance of 102 F g−1 at a current density of 1 A g−1. In a 10,000-cycle test, the electrode demonstrated excellent cycling stability with a capacitance retention rate of 78.5%, which laid the foundation for practical application of the electrode. This work successfully applied N-GQDs in supercapacitors, offering new insights into their development for the energy storage field.
Research on Flower Pattern and Roll Positioning Optimization for Roll Forming Process of TA4 Coiled Tubing
Authors: Deng Xuwen , Hui Songxiao , Ye Xiongyue , Ye Wenjun , Yu Yang , Li Yunbo
Published: December 2024

Titanium alloy coiled tubing (CT) has great superiority while operating in deep wells with severe conditions, but it faces serious challenges during roll forming like high springback, edge cracking and surface wearing. In order to utilize the high precision and high efficiency production of titanium alloy CT, it is urgently necessary to develop a detailed process design. Based on the W-forming strategy and the cubic curve of the strip edge projection, the flower pattern and roll design for the 21-pass roll forming process were completed. Different tube dimensions and roll positionings were researched regarding the aspects of contact state, stress–strain distribution and geometry ovality using numerical simulation. The thickness/diameter ratio of the tube that can be formed should be within an effective range: exceeding the range leads to edge cracking, and an insufficient ratio leads to high springback. Roll positioning significantly affects the contact state and stress distribution, but leads to no major differences in the tube geometry. Finally, the trial production of the TA4 titanium CT with a size of Φ50.8 × 4 mm was completed successfully and was consistent with the simulation results.
Analysis of Honing Material Removal Rate and Surface Quality Using Electroplated Oilstone
Authors: Su Hao , Yang Changyong , Fu Yucan , Nie Rui
Published: December 2024

The manufacturing precision of electro-hydraulic servo valve sleeves is critical to the performance and longevity of the valves. To ensure the service life of these valves, the valve sleeve is typically made from high-hardness martensitic stainless steel, which is considered a hard-to-cut material. Current honing methods often suffer from inefficiency and instability. This study compares the honing processes using electroplated and sintered oilstones, emphasizing processing efficiency and surface quality. Initially, the morphology of the oilstones was examined. Equivalent honing depth and material removal rate per unit width were developed to characterized material removal. The influence of various parameters on honing depth and material removal rates was explored, along with the surface morphology and roughness after honing. The results indicated that electroplated oilstones achieved a material removal rate 2.5 times higher than sintered oilstones. In contrast, sintered oilstones produced superior surface quality. To optimize both surface quality and efficiency, we proposed a sequential honing method: using electroplated oilstones for significant material removal followed by sintered oilstones for surface finishing, which enhanced efficiency by 1.6 times. Electroplated oilstone has broad application prospects in the field of precision and efficient machining of hydraulic components.
Concurrency Conflict Modeling for Asynchronous Processing in Blockchain-Based Transactive Energy Systems
Authors: Marcos Solis Fabiola , Pomares Hernandez Saul Eduardo , Pérez Cruz José Roberto , Rodríguez Henríquez Lil María
Published: December 2024

Blockchain is widely adopted for decentralized transaction management in systems like Transactive Energy (TE). Unfortunately, conventional blockchains with sequential models and restrictive participation rules do not meet energy sector requirements. High volumes of asynchronous-generated transactions impose severe concurrency challenges for blockchain. These difficulties worsen when participants process blocks concurrently, prompting branching and conflicting versions. This issue is often addressed by discarding blocks and reverting transactions, which is detrimental to TE systems. Preserving validated transactions is crucial to avoid disrupting physical asset exchanges and wasting computational resources. To address these challenges, this paper proposes a new model for identifying transaction discrepancies in conflict blocks while maintaining validated transactions. The model enables collaborative block building by integrating multiple blockchain views, eliminating competition, leader selection, and transaction reversals or discards. Block and transaction generation conflicts are addressed by establishing logical-temporal dependencies and leveraging pairwise interactions to detect them toward accelerating consensus. Hence, the model promotes concurrency to enhance transaction processing and avoid resource waste. Simulations indicate traditional models limit network potential to below 5% as blockchain height increases because of single contributions. Conversely, the proposed model uses multiple nodes’ views to achieve up to 90% of the network’s processing capacity.
Evaluating Meniscus, Ligament and Soft Tissue Injury Using MRI in Tibial Plateau Fractures: A Tscherne Classification Approach
Authors: Joo Yong-Bum , Kim Young-Mo , Park Young-Cheol , Chae Soo-Hyeok , Kim Dong-Hwan
Published: December 2024

Background and Objectives: This study investigated associated meniscus and ligament injuries in tibial plateau fractures using magnetic resonance imaging (MRI) and assessed soft tissue injuries in relation to the Schatzker classification and Tscherne classification. Materials and Methods: The data of 185 patients who sustained tibial plateau fractures from January 2010 to April 2021 were retrospectively reviewed. Fractures were classified according to the Schatzker classification system. Soft-tissue injuries were assessed using the Tscherne classification. Menisci and ligaments were evaluated using preoperative MRI. Nerve injuries, compartment syndrome and wound problems were also assessed. The incidence of soft tissue injuries, as well as the relationship between the Schatzker and Tscherne classification systems, were analyzed. Results: Evidence of derangement of meniscus and ligament around the knee was found in 183 (98.9%) patients. The most common injury was a medial collateral ligament injury. The incidence of lateral collateral ligament injury, nerve injury, compartment syndrome and wound problem was higher in high-energy tibial plateau fractures. A tendency was observed between the Schatzker and the Tscherne classifications (p value < 0.001). Higher Tscherne grade was also associated with the incidence of posterior cruciate ligament injury, nerve injury and compartment syndrome. Conclusions: In tibial plateau fractures, soft tissue injuries were highly prevalent. High-energy fractures tended to exhibit higher Tscherne classification grades and showed an increased incidence of meniscus and ligament injuries. The Tscherne classification appears to be a helpful system for predicting soft tissue injuries in tibial plateau fractures. And preoperative MRI can be a helpful tool.
The Impact of Perceived Leadership Effectiveness and Emotional Intelligence on Employee Satisfaction in the Workplace
Authors: Rodrigues Rosa , Teixeira Natália , Costa Bernardo
Published: December 2024

In recent years, the role of leadership in increasing employee satisfaction (ES) has received significant attention, with emotional intelligence (EI) emerging as a key factor in promoting effective leadership (EL) in organizations. Although research has demonstrated the positive impact of EI on workplace outcomes, the interplay between perceived LE, EI, and ES remains underexplored. This research is part of this theme and aims to investigate the influence of the perceived levels of LE and EI of SE leaders in the work environment. This study highlights the importance of integrating emotionally intelligent leaders into organizational structures to improve SE, which subsequently contributes to organizational success. A quantitative methodology was used, with data collected through the application of three questionnaires: one to assess the perception of LE, another to measure EI, and a third to assess SE. The results indicated a positive correlation between all constructs. Employees with more favorable perceptions of their leaders’ EI levels and leadership effectiveness reported greater workplace satisfaction. We also found that LE and EI significantly predicted ES. These findings emphasize the need to foster leadership that is not only competent, but also emotionally intelligent to increase workplace satisfaction and productivity.
Wearable Electrospun Nanofibrous Sensors for Health Monitoring
Authors: Mpofu Nonsikelelo Sheron , Blachowicz Tomasz , Ehrmann Andrea , Ehrmann Guido
Published: December 2024

Various electrospinning techniques can be used to produce nanofiber mats with randomly oriented or aligned nanofibers made of different materials and material mixtures. Such nanofibers have a high specific surface area, making them sensitive as sensors for health monitoring. The entire nanofiber mats are very thin and lightweight and, therefore, can be easily integrated into wearables such as textile fabrics or even patches. Nanofibrous sensors can be used not only to analyze sweat but also to detect physical parameters such as ECG or heartbeat, movements, or environmental parameters such as temperature, humidity, etc., making them an interesting alternative to other wearables for continuous health monitoring. This paper provides an overview of various nanofibrous sensors made of different materials that are used in health monitoring. Both the advantages of electrospun nanofiber mats and their potential problems, such as inhomogeneities between different nanofiber mats or even within one electrospun specimen, are discussed.
Role of Climate and Edaphic Factors on the Community Composition of Biocrusts Along an Elevation Gradient in the High Arctic
Authors: Mas Martinez Isabel , Pushkareva Ekaterina , Keilholz Leonie Agnes , Linne von Berg Karl-Heinz , Karsten Ulf , Kammann Sandra , Becker Burkhard
Published: December 2024

Biological soil crusts are integral to Arctic ecosystems, playing a crucial role in primary production, nitrogen fixation and nutrient cycling, as well as maintaining soil stability. However, the composition and complex relationships between the diverse organisms within these biocrusts are not well studied. This study investigates how the microbial community composition within Arctic biocrusts is influenced by environmental factors along an altitudinal gradient (101 m to 314 m). Metagenomic analyses were used to provide insights into the community composition, revealing that temperature, pH, and nutrient availability significantly shaped the community. In contrast, altitude did not directly influence the microbial composition significantly. Eukaryotic communities were dominated by Chloroplastida and fungi, while Proteobacteria and Actinobacteria prevailed among prokaryotes. Cyanobacteria, particularly orders such as Pseudoanabaenales, Pleurocapsales, and Nostocales, emerged as the most abundant photoautotrophic organisms. Our findings highlight the impact of environmental gradients on microbial diversity and the functional dynamics of biocrusts, emphasizing their critical role in Arctic tundra ecosystems. Arctic biocrusts are intricate micro-ecosystems, whose structure is strongly shaped by local physicochemical parameters, likely affecting essential ecological functions.
Transport of (Micro)plastic Within a River Cross-Section—Spatio-Temporal Variations and Loads
Authors: Chifflard Peter , Nather Thorsten , Weber Collin J.
Published: December 2024

Despite substantial research, the spatio-temporal dynamics of microplastic fluxes remain underexplored, especially in lower-order rivers. This study aims to quantify microplastic loads using a spatio-temporal sampling approach in a single cross-section of the Lahn River, a typical low-mountain river in Central Germany, over a sampling period from July 2020 to April 2021, covering varying discharge conditions, from low to high flow. A total of 198 plastic particles were detected, averaging 3.67 particles per hour, with a mean microplastic load of 0.03 ± 0.027 particles per cubic metre. Microplastic abundance varied spatially within the river cross-section, with lower concentrations found at deeper sampling positions. The data indicate that higher discharge conditions correlate with increased microplastic loads, predominantly at the water surface, suggesting that hydrological conditions significantly influence plastic transport dynamics. However, it remains unclear whether the microplastics observed at higher discharges originate from additional sources or are reactivated from river sediments. This research highlights the need for further studies to validate model assumptions and better understand the reactivation and transport mechanisms of microplastics in river systems.
Path Planning Method for Wire-Based Additive Manufacturing Processes
Authors: Shcherbakov Alexey , Gudenko Alexander , Sliva Andrey , Gaponova Daria , Marchenkov Artem , Goncharov Alexey
Published: December 2024

The relevance of creating specialized computer programs that convert a virtual 3D model of an object into machine code (G-code) for controlling the process of 3D printing products from wire raw materials is substantiated. It is shown that for wire-based additive technologies, a fundamentally important requirement is to ensure the continuity of the surfacing trajectory within one section. A method for determining a continuous surfacing trajectory is proposed, the implementation of which requires two stages: performing a numerical analysis of a two-dimensional region with boundary conditions describing this section; and running a heuristic algorithm for the movement of the surfacing head, in which the direction of movement is selected based on the results of the analysis. The procedure for setting boundary conditions and an algorithm for numerically solving the boundary value problem of determining the field of the “height” function for each section are described. The principles of operation of the heuristic algorithm for selecting the direction of head movement based on the calculated height field and continuous determination of the proximity of adjacent layers and section boundaries are disclosed. An analysis of the algorithm operation is carried out using a section with holes as an example, and the potential of using numerical methods to calculate the change in the temperature field during the surfacing process is shown.
Sphaeropsidin A Loaded in Liposomes to Reduce Its Cytotoxicity and Preserve Antifungal Activity Against Candida auris
Authors: Buonanno Annalisa , Salvatore Maria Michela , Feola Antonia , Siciliano Antonietta , Bellavita Rosa , Imbò Lorenzo Emiliano , Guida Marco , Andolfi Anna , Nicoletti Rosario , Maione Angela , Falanga Annarita , Galdiero Emilia
Published: December 2024

Candida species constitute the most common cause of fungal infections in humans; the emergence of resistance and biofilm formation by Candida species further threaten the limited availability of antifungal agents. Over the past decade, C. auris has caused significant outbreaks worldwide and has emerged as a human pathogenic fungus that causes diseases ranging from superficial to life-threatening disseminated infections. Despite the recent advances in antifungal research, the mechanisms of drug resistance in C. auris remain poorly understood even as its ability to form biofilms poses a significant therapeutic challenge. The purpose of this research was to elucidate the fungal properties of Sphaeropsidin A (SphA), a secondary metabolite derived from Diplodia fungi, with a specific focus on its efficacy against C. auris. This study revealed that SphA and its liposomal encapsulated (SphA-L) form are fungistatic with time-kill kinetics highlighting their efficacy and significantly inhibited the formation of C. auris biofilms. Our investigation into the antifungal mechanism of this drug revealed notable alterations in ROS production and the disruption of the Candida cell cycle. Our findings show that SphA-L impairs key pathogenic traits of C. auris, such as its ability to adhere to human epithelial cell lines, while exhibiting no harmful effects on human cells, highlighting its potential as a future therapeutic agent. In Caenorhabditis elegans infection models, both ShpA and SphA-L displayed effective antifungal activity, significantly reducing the C. auris fungal load and improving nematode survival rates, underscoring their promise as antifungal candidates. Overall, the potent antifungal effects of SphA and SphA-L against C. auris encourage further research.
Lighting the Path: Raman Spectroscopy’s Journey Through the Microbial Maze
Authors: Salbreiter Markus , Frempong Sandra Baaba , Even Sabrina , Wagenhaus Annette , Girnus Sophie , Rösch Petra , Popp Jürgen
Published: December 2024

The rapid and precise identification of microorganisms is essential in environmental science, pharmaceuticals, food safety, and medical diagnostics. Raman spectroscopy, valued for its ability to provide detailed chemical and structural information, has gained significant traction in these fields, especially with the adoption of various excitation wavelengths and tailored optical setups. The choice of wavelength and setup in Raman spectroscopy is influenced by factors such as applicability, cost, and whether bulk or single-cell analysis is performed, each impacting sensitivity and specificity in bacterial detection. In this study, we investigate the potential of different excitation wavelengths for bacterial identification, utilizing a mock culture composed of six bacterial species: three Gram-positive (S. warneri, S. cohnii, and E. malodoratus) and three Gram-negative (P. stutzeri, K. terrigena, and E. coli). To improve bacterial classification, we applied machine learning models to analyze and extract unique spectral features from Raman data. The results indicate that the choice of excitation wavelength significantly influences the bacterial spectra obtained, thereby impacting the accuracy and effectiveness of the subsequent classification results.
Advanced Carbon Nanostructures: Synthesis, Properties, and Applications II
Authors: Kharlamova Marianna V.
Published: December 2024

Single-walled carbon nanotubes [...]
Advancing Towards Higher Contrast, Energy-Efficient Screens with Advanced Anti-Glare Manufacturing Technology
Authors: van der Heijden Danielle , Casimiro Anna , ter Meulen Jan Matthijs , Keskinbora Kahraman , Ercan Erhan
Published: December 2024

The pervasive use of screens, averaging nearly 7 h per day globally between mobile phones, computers, notebooks and TVs, has sparked a growing desire to minimize reflections from ambient lighting and enhance readability in harsh lighting conditions, without the need to increase screen brightness. This demand highlights a significant need for advanced anti-glare (AG) technologies, to increase comfort and eventually reduce energy consumption of the devices. Currently used production technologies are limited in their texture designs, which can lead to suboptimal performance of the anti-glare texture. To overcome this design limitation and improve the performance of the anti-glare feature, this work reports a new, cost-effective, high-volume production method that enables much needed design freedom over a large area. This is achieved by combining mastering via large-area Laser Beam Lithography (LBL) and replication by Nanoimprint Lithography (NIL) processes. The environmental impact of the production method, such as regards material consumption, are considered, and the full cycle from design to final imprint is discussed.
Heterogeneity in Health Outcomes in the Strong Hearts, Healthy Communities-2.0 Multilevel Intervention in a Community-Randomized Trial: An Exploratory Study of Moderators
Authors: Rethorst Chad D. , Demment Margaret M. , Ha Seungyeon , Folta Sara C. , Graham Meredith L. , Eldridge Galen D. , Seguin-Fowler Rebecca A.
Published: December 2024

Background/Objectives: Multilevel interventions have demonstrated efficacy in improving obesity and other related health outcomes. However, heterogeneity in individual responses indicates the need to identify the factors associated with responses and non-responses to multilevel interventions. The objective of this report is to identify the potential sources of heterogeneity through the exploration of the moderation effects of participant characteristics (sociodemographic and baseline physical/mental health) in the Strong Hearts, Healthy Communities-2.0 (SHHC-2.0) intervention. Methods: SHHC-2.0 is a 24-week multilevel intervention to improve people’s diet and physical activity evaluated using a cluster-randomized, controlled trial design conducted with women aged 40 and older living in rural communities with an elevated risk of cardiovascular disease, defined as having a BMI > 30, or a BMI 25–30 plus < 1 weekly occurrence of 30 min of physical activity during leisure time. Linear mixed models were used to compare the between-group changes in the outcomes (weight, systolic blood pressure, hemoglobin A1c [HbA1c], and triglycerides), with an interaction term included for each potential moderator. Results: Within the sociodemographic characteristics, there were no differences in effectiveness by age, income, or baseline BMI status, but the participants with a high school education or less experienced less weight loss. Among their health history, only a history of hypertension was associated with differential outcomes; those with a history of hypertension demonstrated a greater reduction in systolic blood pressure. The participants with elevated depressive symptoms demonstrated greater weight loss and a greater reduction in the HbA1c level. Conclusions: SHHC-2.0 was effective across a wide range of participants. The identified moderators (i.e., education level) may inform the future tailoring of the SHHC intervention to optimize the outcomes among participant subgroups, while more broadly, our findings can serve to inform the development and dissemination of multilevel interventions.
Genetic Diversity of Cryptosporidium Species in Different Hosts in Africa: A Systematic Review
Authors: Midala Chahari A. , Kyari Falmata , Onyiche ThankGod E.
Published: December 2024

Cryptosporidium species are protozoan parasites of veterinary and medical importance that infect a wide range of vertebrates globally. Primarily, the mode of infection is typically ingestion of sporulated oocysts, and the risk of transmission to susceptible host is increased by environmental contamination with sporulated oocysts. This systematic review aims to provide a summary of the available data on Cryptosporidium genotypes in Africa. A systematic research of literature on four electronic databases, including Scopus Web of Science, Science Direct, and AJOL, was performed for the determination of Cryptosporidium genotypes in animal and human hosts across Africa between January 2000 to August 2023. All published studies were screened based on the inclusion and exclusion criteria. Overall, 131 Cryptosporidium genotypes were generated from the 47 articles included in this study, which cut across 14 African countries. Cryptosporidium genotypes were reported across all regions within the African continent, such as northern, eastern, western, central, and southern Africa. Cryptosporidium hominis (Ia, Ib, Id, Ie, If, Ih, Ii, and Ik) genotypes were the most dominant, with C. hominis subfamily Ia genotypes reported across all African regions, which indicates high diversity of these subfamilies on the continent. Cryptosporidium parvum (IIa, IIb, IIc, IId, IIe, IIm, and IIi) genotypes were the second dominant genotypes reported, with C. parvum subfamily IIa genotypes having a high diversity across all the African regions with the exception of southern Africa. C. parvum subfamily IIc (IIcA5G3b), which is known to circulate among humans, was reported in a study on dogs in Nigeria. Other documented species of Cryptosporidium with known subtypes in Africa include C. meleagridis (IIIb, IIId, and IIIg), C. cuniculus (Vb), C. ubiquitum (XIIa), C.), and C. erinacei (XIIIa) genotypes. In conclusion, findings from this review have elucidated the Cryptosporidium species and subtypes within the African continent across humans and multiple animal hosts, implying the diversity of this parasites and its ability to infect wide host ranges.
Respiratory Pathogen Coinfection During Intersecting COVID-19 and Influenza Epidemics
Authors: Jiang Lina , Jin Yifei , Li Jingjing , Zhang Rongqiu , Zhang Yidun , Cheng Hongliang , Lu Bing , Zheng Jing , Li Li , Wang Zhongyi
Published: December 2024

Respiratory pathogen coinfections pose significant challenges to global public health, particularly regarding the intersecting epidemics of COVID-19 and influenza. This study investigated the incidences of respiratory infectious pathogens in this unique context. We collected throat swab samples from 308 patients with a fever from outpatient and emergency departments at sentinel surveillance hospitals in Xiamen, southeast of China, between April and May 2023, testing for SARS-CoV-2 and 26 other respiratory pathogens. The coinfection rate of the XBB SARS-CoV-2 variant with other respiratory pathogens was higher than that observed during the Alpha and Delta phases. Among patients with influenza, bacterial coinfections were more prevalent. Only 0.65% (2/308) of the patients were concurrently infected with both COVID-19 and influenza. Age-stratified analysis showed a clear pattern, with a higher incidence of coinfections in children under 18 years of age. These findings highlight the need for the timely detection of respiratory pathogen coinfections and for the implementation of appropriate interventions, crucial for reducing disease burden during intersecting respiratory epidemics.
High Carbonyl Graphene Oxide Suppresses Colorectal Cancer Cell Proliferation and Migration by Inducing Ferroptosis via the System Xc−/GSH/GPX4 Axis
Authors: Zhou Xiecheng , Zhang Qixing , Zhu Haoran , Ouyang Guangxiong , Wang Xin , Cai Yuankun
Published: December 2024

Background/Objectives: Colorectal cancer (CRC) is characterized by a high rate of both incidence and mortality, and its treatment outcomes are often affected by recurrence and drug resistance. Ferroptosis, an iron-dependent programmed cell death mechanism triggered by lipid peroxidation, has recently gained attention as a potential therapeutic target. Graphene oxide (GO), known for its oxygen-containing functional groups, biocompatibility, and potential for functionalization, holds promise in cancer treatment. However, its role in ferroptosis induction in CRC remains underexplored. The objective of this study was to investigate the effects of High Carbonyl Graphene Oxide (HC-GO) on ferroptosis in CRC and elucidate the underlying mechanisms. Methods: In vitro assays were conducted to evaluate the impact of HC-GO on CRC cell proliferation, mitochondrial function, iron accumulation, lipid peroxidation, and reactive oxygen species (ROS) production. The ferroptosis inhibitor Fer-1 was used to confirm the role of ferroptosis in HC-GO’s anti-tumor effects. In vivo, the anti-tumor activity of HC-GO was assessed in a CRC xenograft model, with organ toxicity evaluated. Results: HC-GO significantly inhibited CRC cell proliferation, induced mitochondrial damage, and enhanced iron accumulation, lipid peroxidation, and ROS production. It also downregulated the ferroptosis-inhibiting proteins GPX4 and SLC7A11, which were reversed by Fer-1, confirming the involvement of ferroptosis in HC-GO’s anti-cancer effects. In vivo, HC-GO significantly suppressed tumor growth without noticeable toxicity to vital organs. Conclusions: HC-GO triggered ferroptosis in CRC cells by suppressing the System Xc−/GSH/GPX4 pathway, providing a novel therapeutic strategy for CRC treatment. These findings suggest HC-GO as a promising nanomedicine for clinical application, warranting further investigation to explore its potential in CRC therapy.
Natural Gomesin-like Peptides with More Selective Antifungal Activities
Authors: Bolosov Ilia A. , Finkina Ekaterina I. , Bogdanov Ivan V. , Safronova Victoria N. , Panteleev Pavel V. , Ovchinnikova Tatiana V.
Published: December 2024

Background: Antimicrobial peptides are generally considered promising drug candidates for combating resistant bacterial infections. However, the selectivity of their action may vary significantly. Natural gomesin, isolated from haemocytes of the tarantula Acanthoscurria gomesiana, demonstrates a broad spectrum of antimicrobial activities, being the most effective against pathogenic fungi. Methods: Here, we searched for variants of natural gomesin-like peptides and produced their recombinant analogs in the bacterial expression system. The antimicrobial activities of the obtained peptides were tested against a panel of bacterial and yeast strains, and their toxicity towards human cells was examined. Results: Most of the new analogs of gomesin have primary structures homologous to that of the natural gomesin; however, they have fewer amino acid residues and post-translational modifications. One of the discovered analogs, the His-rich shorter peptide from the spider Dysdera sylvatica, designated as DsGom, displays antifungal activity comparable with that of natural gomesin. In the process of the structural–functional study of DsGom, it was shown that this analog retains a basic mechanism of action similar to that of natural gomesin. The DsGom analog has a significantly better toxicity profile as compared to gomesin. At the same time, the loss of the first Arg residue reduces, but does not annul, the antifungal activity of DsGom. Moreover, the acidification of the growth medium reduces the loss of the antifungal activity of this analog. Conclusions: The discovered natural gomesin-like peptides display more selective antifungal activities as compared to gomesin. The low cytotoxicity of DsGom, combined with its high antifungal activity and stability, allows us to consider it a promising drug candidate for the treatment of fungal infections, especially those caused by fungi of the Candida genus.