bluuebunny/crossref_metadata_2025 huggingface dataset:
Face Mask Reduces the Effect of Proposer’s (Un)Trustworthiness on Intertemporal and Risky Choices
Authors: Cannito Loreta , Anzani Stefano , Bortolotti Alessandro , Di Domenico Alberto , Palumbo Riccardo
Published: June 2022

Previous literature suggested that individuals increase temporal and risk discounting at the presence of a proposer whose face is perceived as untrustworthy, suggesting the activation of protective choice patterns. By the way, the COVID-19 pandemic has substantially transformed the way we interact with other people, even bringing us into situations where the face of the person making a proposal is not fully visible, because of the mask. With the current study, we aimed at verifying if the effect of proposer’s facial (un)trustworthiness on discounting behavior is modulated by mask wearing. In two different experiments, participants performed traditional delay and probability discounting tasks with masked proposers manipulated across trustworthiness levels. Results highlighted that, even after checking for subject-specific emotion recognition ability with masked faces, the presence of a masked untrustworthy proposer increases both delay and probability discounting parameters, although the effect is not statistically significant and smaller than the one detected at the presence of an untrustworthy proposer without a mask. These results suggest that the ability to perceive the proposer’s (un)trustworthiness is affected by the mask, with a consequent less strong effect of proposer’s (un)trustworthiness on choice behavior on both intertemporal and risky choices. Limits and possible implications are outlined and discussed.
System Mapping of Antimicrobial Resistance to Combat a Rising Global Health Crisis
Authors: Matthiessen Lea Ellen , Hald Tine , Vigre Håkan
Published: June 2022

Antimicrobial resistance (AMR) decreases the effectiveness of antimicrobials to treat bacterial infections in humans and animals. The increased occurrence of AMR in bacterial population in humans, animals, and the environment requires the measures to combat a rising global health crisis. The aim of this research was to present current knowledge on AMR in a system map and to identify potential explanations of former identified variables significantly associated with AMR. This study applies a systems thinking approach and uses feedback loops to visualize the interconnections between human, animal, and environmental components in a circular AMR system map model. First, a literature review focusing on AMR and socioeconomic factors, wicked problem, and system change was carried out, which was then processed in a system map to conceptualize the present core challenges of AMR via feedback loops. Second, to investigate possible underlying values of the society and those that influence humans' behavior in the present AMR system, an iceberg model was established. Third, leverage points were assessed to estimate which kinds of interventions would have the greatest effect to mitigate AMR in the system. The present AMR system map implies the potential to identify and visualize important risk factors that are direct or indirect drivers of AMR. Our results show that the tool of system mapping, which interconnects animals, humans, and environment in one model, can approach AMR holistically and be used to assess potential powerful entry points for system wide interventions. This study shows that system maps are beneficial as a model to predict the relative effect of different interventions and adapt to rapidly changing environments in a complex world. Systems thinking is considered as a complementing approach to the statistical thinking, and further research is needed to evaluate the use of such tools for the development and monitoring of interventions.
A Prospectively Validated Nomogram for Predicting the Risk of PHQ-9 Score ≥15 in Patients With Erectile Dysfunction: A Multi-Center Study
Authors: Zheng Yu , Gao Ming , Hou Guangdong , Hou Niuniu , Feng Xiao , Jannini Tommaso B. , Wei Di , Zheng Wanxiang , Zhang Lei , Dun Xinlong , Zhang Geng , Wang Fuli , Meng Ping , Jannini Emmanuele A. , Yuan Jianlin
Published: June 2022

Background

Although erectile dysfunction (ED) often occurs simultaneously with depression, not all patients with ED suffer major depression (MD), with a PHQ-9 score ≥15 indicating MD. Because the PHQ-9 questionnaire includes phrases such as “I think I am a loser” and “I want to commit suicide,” the psychological burdens of ED patients are likely to increase inevitably after using the PHQ-9, which, in turn, may affect ED therapeutic effects. Accordingly, we endeavored to develop a nomogram to predict individual risk of PHQ-9 score ≥15 in these patients.

Methods

The data of 1,142 patients with ED diagnosed in Xijing Hospital and Northwest Women and Children's Hospital from January 2017 to May 2020 were analyzed. While the Least Absolute Shrinkage and Selection Operator regression was employed to screen PHQ-9 score ≥15 related risk factors, multivariate logistic regression analysis was performed to verify these factors and construct the nomogram. The training cohort and an independent cohort that comprised 877 prospectively enrolled patients were used to demonstrate the efficacy of the nomogram.

Results

The IIEF-5 score, PEDT score, physical pain score, frequent urination, and feeling of endless urination were found to be independent factors of PHQ-9 score ≥15 in patients with ED. The nomogram developed by these five factors showed good calibration and discrimination in internal and external validation, with a predictive accuracy of 0.757 and 0.722, respectively. The sensitivity and specificity of the nomogram in the training cohort were 0.86 and 0.52, respectively. Besides, the sensitivity and specificity of the nomogram in the validation cohort were 0.73 and 0.62, respectively. Moreover, based on the nomogram, the sample was divided into low-risk and high-risk groups.

Conclusion

This study established a nomogram to predict individual risk of PHQ-9 score ≥15 in patients with ED. It is deemed that the nomogram may be employed initially to avoid those with a low risk of MD completing questionnaires unnecessarily.
Exploring Clinical Risk Factors for Breast Cancer Among American Indian Women
Authors: Nadeau Melanie , Best Lyle G. , Klug Marilyn G. , Wise Kathryn
Published: June 2022

Objective

Very little is known about the breast cancer risk profile among American Indian women. Previous research shows that the proportion of American Indian/Alaska Native women with baseline characteristics (commonly known breast cancer risk factors) differs from other ethnicities. This retrospective case control study was designed to the explore the association of these factors among American Indian women with and without breast cancer.

Methods

Cases and controls were retrospectively selected from the medical records of American Indian women who obtained their health care from Quentin N. Burdick Memorial Health Care Facility (Indian Health Service) in Belcourt, ND. For each woman with breast cancer (n = 141), two controls were selected when possible (n = 278). Risk factors examined included woman's age, age at first live birth, age of menarche, the number of previous benign breast biopsies, the total number of first-degree relatives with breast cancer, body mass index and parity. Odds ratios and 95% confidence intervals were calculated using logistic regression.

Results

Many of the associations found among American Indian women who obtained their health care from Quentin N. Burdick Memorial Health Care Facility (Indian Health Service) in Belcourt, ND, between risk factors commonly identified in other populations and breast cancer were weakly positive. Nulliparity was the only risk factor to consistently show a positive significant association (OR = 2.87, 95% CI 1.16–0.7.12).

Conclusion

Disparities in breast cancer incidence, mortality and screening among Northern Plains American Indian emphasize the need to better understand the risk factors associated with breast cancer in this population. Based on the results of this study, the value of current risk prediction models in American Indian communities is uncertain and clinicians should be cautious in using these models to inform American Indian patients of their risk for breast cancer.
The Moderating Effect of COVID-19 Risk Perception on the Relationship Between Empathy and COVID-19 Volunteer Behavior: A Cross-Sectional Study in Jiangsu, China
Authors: Zhu Yeyang , Zhuang Jie , Liu Baohua , Liu Huan , Ren Jiaojiao , Zhao Miaomiao
Published: June 2022

The health system has encountered great challenges since the COVID-19 outbreak, volunteers are urgently needed in every situation during this crisis. The current study aimed to explore the relationship between empathy and COVID-19 volunteer behavior, along with the moderating role of COVID-19 risk perception in the above relationship. The cross-sectional survey was conducted online using Wenjuanxing from February 12th to March 16th, 2021, in Jiangsu, China. A total of 1,486 participants completed the Toronto Empathy COVID-19 volunteer behavior and COVID-19 risk perception questionnaires. The SPSS PROCESS macro was yielded to examine the moderating effect. Simple slopes analysis was conducted to detect the associations between empathy and COVID-19 volunteer behavior at three levels of the COVID-19 risk perception. The Johnson-Neyman (J-N) technique was used to calculate where the moderating effect is significance. Results showed that empathy was positively related with COVID-19 volunteer behavior (β= 0.080, p < 0.001). COVID-19 risk perception played a moderation effect on association between empathy and COVID-19 volunteer behavior (β = −0.005, p < 0.001), the greater the levels of COVID-19 risk perception, the weaker the associations between empathy and COVID-19 volunteer behavior. The J-N test showed the association between empathy and COVID-19 volunteer behavior was no longer significant when values of COVID-19 risk perception was >10.71. Current findings could enlighten researchers and policy makers, that fostering volunteerism among public during crisis situation through arousing more empathy and reducing unnecessary risk perception of the public.
Factors Associated With Late Antiretroviral Therapy Initiation Among People Living With HIV in Southern Iran: A Historical Cohort Study
Authors: Afrashteh Sima , Fararouei Mohammad , Ghaem Haleh , Gheibi Zahra
Published: June 2022

Objectives

Late initiation of antiretroviral therapy (ART) is associated with poor outcome among people living with HIV (PLHIV) and higher risk of transmission of infection. This study was conducted to identify the determinants of late ART initiation among PLHIV in Southern Iran.

Methods

A historical cohort study was conducted on 1,326 PLHIV of ≥15 years of age who were registered with the behavioral diseases counseling center (BDCC) in southern Iran from August 1997 to March 2021. Late ART initiation was defined as a CD4 cell count <200 cells/mm3 or having a clinical AIDS diagnosis at the time of ART initiation. The required demographic and clinical data were collected from the patients' medical records. Multiple regression analysis was conducted to define late ART initiation associated factors.

Results

Late ART initiation was found among 81.9% of patients. Based on the results of the multivariate analysis, older age (odds ratio [OR] = 1.02, 95% CI = 1.00–1.04), being single (ORsingle/married = 1.80, 95% CI = 1.17–2.78), history of drug use (ORyes/no = 1.64, 95% CI = 1.02–2.62), year of ART initiation (OR2011−2013/2018−2021 = 3.65, 95% CI = 2.28–5.86), and possible route of transmission (ORdruginjection/sexual = 7.34, 95% CI = 1.16–46.21) were directly associated with the risk of late ART initiation.

Conclusions

The results show that the prevalence of late ART initiation was alarmingly high. For better infection control and better prognosis of infection, people at high risk need to be provided with timely services (e.g., diagnosis, treatment, training, and social support).
Changing Conditions for Local Food Actors to Operate Towards Agroecology During the COVID-19 Pandemic
Authors: Frank Markus , Kaufmann Brigitte , Ejarque Mercedes , Lamaison María Guadalupe , Nessi María Virginia , Amoroso Mariano Martin
Published: June 2022

Given the novel character of disturbances caused by the pandemic in food systems, initial studies have been conducted to stress the reinforced urgent need for food systems' transformation toward sustainability. First assessments, conducted in the early months of the pandemic, found that local food actors responded to changing production and marketing conditions by implementing alternative practices under the umbrella of agroecology. However, given the unprecedented and dynamic character of the pandemic in regional situations, and related context-specific changes caused in food system actors' operations, case studies are needed to assess in more detail under which changing conditions food actors implemented alternative practices. Moreover, the maintenance of practices as conditions normalize, and food actors' transformative potential in relation to the principles of agroecology, need further assessment. In response to these emerging issues, we provide insights into our case study research conducted during 2021 in a local food system in Argentina. The aim of this research was to study how changing conditions triggered local food actors to (re-)frame their objectives and activities regarding marketing, and to assess the relevance of agroecological principles as a means of responding to changing conditions and to unfold longer-term transitions. We identified local producer shops (n= 5) and markets (n= 4) that were established or consolidated by self-organized producer groups (SOPGs) during the first months of the pandemic. Using semi-structured interviews with SOPG members (n= 12) and qualitative content analysis, we found that alternative practices were adopted in response to different changing conditions, and new needs and opportunities for producers and consumers brought about by the pandemic. Objectives pursued, and activities undertaken by the groups revealed reactive short-term mitigation strategies, and proactive longer-term transformative objectives. The relational analysis between practices and agroecological principles showed that the principles became important means of responding to changing conditions and to unfold longer-term transitions. The cases illustrate how local food actors operationalized agroecological principles, and in turn how principles can be used to investigate the nature and potentials of food actors' alternative practices, highlighting the relevance of agroecology to co-design sustainability transitions in local food systems and to mitigate possible future crisis.
Case Report: Upper Thoracic Purely Extradural Spinal Meningioma With Nerve Root Attachment: A Case Report and Literature Review
Authors: Wang Zhao-Lin , Mou Jian-Hui , Sun Dong , Liu Peng
Published: June 2022

Introduction

We describe a case of purely extradural spinal meningioma (EDSMs) with nerve root attachment and present the clinical profiles, radiological findings, operative management, and follow-up data, along with a literature review. This case study is purely extradural spinal meningioma attached to the nerve root, and the available literature review discusses the diagnosis and treatment. Purely epidural spinal meningiomas are extremely rare, and only a few clinical reports are published on this disease. Although epidural meningioma is a benign tumor, the recurrence rate is higher when compared with ordinary meningioma.

Case study

A 39-year-old male complained of chest and back pain with ring-shaped radiations to the precordial area and numbness in both the lower extremities. After a long walk, he felt disharmony in both his lower limbs. Magnetic resonance imaging showed a mass located on the left posterolateral side of the T1–T3 spinal cord and exhibited a dumbbell-type growth outward at the level of the T2–T3 intervertebral foramen. On the left side of the thoracic, a hemilaminectomy procedure was performed. The tumor was found in the ventral side of the left nerve root of T1 and was surrounded by the left nerve root of T2. To obliterate the tumor, the T2 nerve root was severed. The patient was advised to come for the follow-up on the 3rd, 6th, and 12th months postoperatively, and there were no complaints or signs of recurrence.

Conclusion

Purely extradural spinal meningioma with nerve root attachment is rare and has no clinical symptoms and image findings. To completely resect the lesion and avoid recurrence, the affected nerve root, epidural fat tissue, and nerve root sheath should be extensively resected and burned, coagulating the adjacent ventral posterior longitudinal ligament.
A Practical Nomogram and Risk Stratification System Predicting Cancer-Specific Survival for Hepatocellular Carcinoma Patients With Severe Liver Fibrosis
Authors: Yang Dashuai , Su Yang , Zhao Fangrui , Chen Chen , Zhao Kailiang , Xiong Xiangyun , Ding Youming
Published: June 2022

Objective

Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related deaths worldwide. This study aims to construct a novel practical nomogram and risk stratification system to predict cancer-specific survival (CSS) in HCC patients with severe liver fibrosis.

Methods

Data on 1,878 HCC patients with severe liver fibrosis in the period 1975 to 2017 were extracted from the Surveillance, Epidemiology, and End Results database (SEER). Patients were block-randomized (1,316 training cohort, 562 validation cohort) by setting random seed. Univariate and multivariate COX regression analyses were employed to select variables for the nomogram. The consistency index (C-index), the area under time-dependent receiver operating characteristic curve (time-dependent AUC), and calibration curves were used to evaluate the performance of the nomogram. Decision curve analysis (DCA), the C-index, the net reclassification index (NRI), and integrated discrimination improvement (IDI) were used to compare the nomogram with the AJCC tumor staging system. We also compared the risk stratification of the nomogram with the American Joint Committee on Cancer (AJCC) staging system.

Results

Seven variables were selected to establish the nomogram. The C-index (training cohort: 0.781, 95%CI: 0.767–0.793; validation cohort: 0.793, 95%CI = 95%CI: 0.779–0.798) and the time-dependent AUCs (the training cohort: the values of 1-, 3-, and 5 years were 0.845, 0.835, and 0.842, respectively; the validation cohort: the values of 1-, 3-, and 5 years were 0.861, 0.870, and 0.876, respectively) showed satisfactory discrimination. The calibration plots also revealed that the nomogram was consistent with the actual observations. NRI (training cohort: 1-, 2-, and 3-year CSS: 0.42, 0.61, and 0.67; validation cohort: 1-, 2-, and 3-year CSS: 0.26, 0.52, and 0.72) and IDI (training cohort: 1-, 3-, and 5-year CSS:0.16, 0.20, and 0.22; validation cohort: 1-, 3-, and 5-year CSS: 0.17, 0.26, and 0.30) indicated that the established nomogram significantly outperformed the AJCC staging system (P < 0.001). Moreover, DCA also showed that the nomogram was more practical and had better recognition.

Conclusion

A nomogram for predicting CSS for HCC patients with severe liver fibrosis was established and validated, which provided a new system of risk stratification as a practical tool for individualized treatment and management.
Clinical Study on the Application of Preserved Urethral Mucosa at the Prostatic Apex in Transurethral Plasmakinetic Resection of the Prostate
Authors: Liang Jun-Qiang , Ma Wang-Teng , Lu Bin-Wei , Dai Liang , Zhao Yu-Ming , Zhang Ji-Dong , Tian Bao , Liu Qing-Li
Published: June 2022

Objective

To explore the differences in the clinical efficacy, complications, and safety of transurethral plasmakinetic resection of the prostate (PKRP) by the conventional approach versus the approach preserving the urethral mucosa at the prostatic apex in the treatment of benign prostatic hyperplasia (BPH).

Methods

A total of 90 patients with PKRP admitted to the First Hospital of Qinhuangdao from December 2018 to March 2021 were selected and divided into a control group (conventional PKRP, n = 45) and an observation group (PKRP with preserved urethral mucosa at the prostatic apex, n = 45). The clinical efficacy, safety, and sexual function of the groups were evaluated using the patients’ International Prostate Symptom Score (IPSS), quality of life (QoL), prostate volume, maximum flow rate (Qmax), post-void residual (PVR), blood loss, surgical resection efficiency, and surgical complication data.

Results

The differences in the preoperative indicators, glandectomy quality, and glandectomy rate between the groups were not statistically significant (P > 0.05). However, in the observation group, the surgery time and blood loss were significantly lower compared with the control group, and the resection efficiency was significantly higher, with statistical significance (P < 0.05). In the follow-up, one month after surgery, the IPSS and QoL were lower in the observation group than in the control group, and the differences were statistically significant (P < 0.05); three months after surgery, the PVR, IPSS, QoL, and Qmax scores were similar between the groups, with no statistical significance (P > 0.05). In terms of surgical complications, the incidences of urinary incontinence and other complications after catheter extraction were significantly lower in the observation group than in the control group, and the differences between the groups were statistically significant (P < 0.05).

Conclusion

Compared with conventional PKRP, PKRP with preserved urethral mucosa at the prostatic apex can lead to immediate urinary continence after catheter extraction, reduce intraoperative blood loss, and shorten the surgery time, thus improving the surgical efficiency.
Mathematical Modelling of Pseudomonas aeruginosa L-forms Reveals Complex Interplay Between Host Defence Mechanisms and Putative Treatments
Authors: Spalding Chloe , Shirgill Sandeep , Taylor Emma , Krachler Anne-Marie , Jabbari Sara
Published: June 2022

The bacterium Pseudomonas aeruginosa has been shown to undergo a morphological transition akin to L-forms under exposure to antibiotics, a process which may contribute to persistent infections. With the further consideration of antibiotic-resistance mechanisms, this transition renders the design of effective treatment strategies challenging. Through a mathematical model, we illustrate that additionally incorporating the complexities of the host immune response can render somewhat surprising predictions from the simulations. In particular, scenarios arise whereby the addition of a treatment strategy to directly target the L-forms results in a worsened infection, while in others this treatment could turn an antibiotic-resistant infection from persistent to treatable. The study highlights the importance of understanding the in vivo interplay between immune cells and pathogens for successful treatment design.
Withaferin A Inhibits Neutrophil Adhesion, Migration, and Respiratory Burst and Promotes Timely Neutrophil Apoptosis
Authors: Bayless Rosemary L. , Sheats M. Katie , Jones Samuel L.
Published: June 2022

Neutrophils play a major role in many equine conditions, including equine asthma, laminitis, and intestinal ischemia and reperfusion injury, and therefore represent an attractive target for innovative therapeutic approaches. Novel strategies for reducing neutrophilic inflammation include modulation of neutrophil functions and lifespan. Withaferin A (WFA) is a phytochemical with well-establishedin vitroandin vivoanti-inflammatory properties, but its direct effects on neutrophils are largely unknown. We hypothesized that WFA would inhibit adhesion, migration, and respiratory burst by equine neutrophils and promote timely apoptosis of primed equine neutrophils. Consistent with this hypothesis, our data show that WFA causes a significant, concentration-dependent inhibition of equine neutrophil adhesion, migration, and respiratory burst in response to diverse stimuli. Further, WFA treatment increased apoptosis of equine neutrophils exposed to GM-CSF for 24 h. This pro-apoptotic effect of WFA was not observed in unprimed neutrophils, nor at the 2-h time point relevant to our functional neutrophil experiments. Our data demonstrate that WFA may reduce neutrophil-mediated inflammation through multiple mechanisms, including suppression of inflammatory responses and promotion of apoptosis. Additional research is needed to elucidate the molecular mechanisms for these effects and evaluate the potential clinical use of WFA in veterinary and human patients.
Assessing Amino Acid Metabolism in Splanchnic Tissues and Mammary Glands to Short-Term Graded Removal of Lys From an Abomasal-Infused Amino Acid Mixture in Lactating Goats
Authors: Li Yantao , Lin Xueyan , Liu Chen , Hu Zhiyong , Hou Qiuling , Wang Zhonghua
Published: June 2022

To investigate the responses of amino acid metabolism in portal-drained viscera (PDV), liver, and mammary glands (MGs) to a graded gradual decrease of post-ruminal Lys supply, four multi-catheterized lactating goats were used in a 4 × 4 Latin square experiment. Goats were fasted for 12 h and then received a 33-h abomasal infusion of an amino acid mixture and glucose. Treatments consisted of a graded decrease of Lys content in the infusate to 100 (complete), 60, 30, or 0% as in casein. Lys-removed infusions decreased the production of milk, milk protein, fat, and lactose linearly and also decreased arterial Lys concentrations linearly (p< 0.05). Net PDV uptake decreased linearly (p< 0.05) with decreasing PDV loss ratio (p< 0.05). Although liver removal of Lys decreased linearly (p< 0.05), the removal ratio relative to portal absorption changed small, which was about 10% in all four treatments. Reduced Lys supply resulted in a linear decrease in the utilization of Lys in the peripheral tissues (except mammary,p< 0.05) and the release of more Lys in MGs. Although net mammary uptake of Lys declined linearly (p< 0.05), lactating goats can partially offset the negative effect of decreased circulating Lys concentrations by increasing mammary affinity (p< 0.05) and increasing mammary blood flow (p< 0.05). Graded removal of Lys from the infusate linearly decreased mammary uptake-to-output ratios of Lys (p< 0.05) suggesting that mammary catabolism of Lys decreased. Meanwhile, the treatments linearly increased circulating concentrations of glucagon and linearly decreased prolactin (p< 0.05). In conclusion, the results of the present study indicated that there were several mechanisms used to mitigate a Lys deficiency, including reduced catabolism of Lys in PDV and peripheral tissues (including MGs) and linearly increased mammary blood flow and mammary affinity together with increased mammary uptake and U:O of branched-chain amino acids (BCAA). Given these changes, the decline in milk protein production could be attributed to the combined effect of mass action with Lys and hormonal status.
Strand-Specific Patterns of Codon Usage Bias Across Cressdnaviricota
Authors: Crespo-Bellido Alvin , Duffy Siobain
Published: June 2022

The rapidly expanding phylum Cressdnaviricota contains circular, Rep-encoding single-stranded (CRESS) DNA viruses that are organized within seven established families, but many CRESS DNA virus sequences are not taxonomically defined. We hypothesized that genes in CRESS DNA virus ambisense genomes exhibit strand-specific signatures due to a cytosine to thymine transition bias that can help determine the orientation of the genome: which strand is packaged and is in the “virion sense”. To identify broad strand-specific patterns across genera, we performed compositional analyses of codon usage across the two major opposite sense open reading frames of 712 reference viruses. Additionally, we developed a statistical test to identify relative codon overrepresentation between ambisense sequence pairs for each classified virus exemplar and an additional 137 unclassified CRESS DNA viruses. Codons clustered by the identity of their third-position nucleotide, displaying both strand- and genus-specific patterns across Cressdnaviricota. Roughly 70% of virion-sense sequences have a relative overrepresentation of thymine-ending codons while ~80% of anti-sense sequences display a relative overrepresentation of adenine-ending codons (corresponding to a relative overrepresentation of thymine in these genes as packaged). Thirteen of the 137 unclassified viruses show strong evidence of having the rarer circovirus-like genome orientation, and likely represent novel genera or families within Cressdnaviricota. Given the strong strand-specific patterns of relative codon overrepresentation, the results suggest that the relative codon overrepresentation test can serve as a tool to help corroborate the genome organization of unclassified CRESS DNA viruses.
Genomic Landscape of Metastatic Lymph Nodes and Primary Tumors in Non-Small-Cell Lung Cancer
Authors: Chen Bing , Li Rutao , Zhang Junling , Xu Lin , Jiang Feng
Published: June 2022

Objective: To investigate the genetic mutation characteristics of non-small cell lung cancers (NSCLC) with and without lymph node metastasis.

Methods: Primary lesions and metastatic lymph node lesions of 36 Chinese NSCLC patients were tested for somatic mutations, tumor mutation burden, phylogenetic and clonal evolutional analysis using a 1021-gene panel by next-generation sequencing (NGS) with an average sequencing depth of 671X.

Results: In this study, eighteen patients with lung adenocarcinoma (LUAD) and 18 with lung squamous cell carcinoma (LUSC) were included. Different groups had distinct characteristics of gene mutations. CTNNB1 gene mutations were only present in Nome_LC LUAD patients (p < 0.05). ARID1A mutation was however the only gene with significant alterations (p < 0.05) in Nome_LC in LUSC. Phylogenetic trees of mutated genes were also constructed. Linear and parallel evolutions of metastatic lymph nodes were observed both in LUAD and LUSC.

Conclusion: LUSC exhibited more genetic mutations than LUAD. Intriguingly, there was significant difference in gene mutations between Meta_LC and Nome_LC. CTNNB1 gene alteration was the key mutation in LUAD that seems to promote proliferation of the tumor and then determine T stage. On the other hand, proliferation of the tumor was characterized by ARID1A missense mutation in LUSC, thus influencing the T stage as well. Lymph node metastasis could display both linear and parallel evolutionary characteristics in NSCLC. Different metastatic lymph nodes might have exactly the same or different mutated genes, underlining the heterogeneous genomic characteristics of these cancer types.
A Design of Biomimetic Prosthetic Hand
Authors: Narumi Sakura , Huang Xiansong , Lee Jongho , Kambara Hiroyuki , Kang Yousun , Shin Duk
Published: June 2022

Many patients with upper limb defects desire myoelectric prosthetic hands, but they are still not used for some reasons. One of the most significant reasons is its external appearance, which has the discomfort caused by the structural difference between a human hand and a robotic link. The structure must be based on human anatomy to create a more natural-looking prosthesis. This study designed a biomimetic prosthetic hand with bones, ligaments, tendons, and multiple muscles based on the human musculoskeletal system. We verified the proposed prosthetic hand using the viscoelastic angle sensor to determine whether it works like a human hand. We also compared the finger force of the prosthetic hand with that of a human finger. It could be capable of controlling the angle and the stiffness of the joint by multiple extensor and flexor muscles, like humans.
Performance Comparison of Control Strategies for a Variable-Thrust Solid-Propellant Rocket Motor
Authors: Cha Jihyoung , de Oliveira Élcio Jeronimo
Published: June 2022

This paper deals with a performance comparison of the control algorithm for a variable-thrust solid-propellant rocket motor (VTSRM). To do this, we develop a simulation model of a VTSRM considering characteristic changes in the combustor and design control systems for pressure and thrust. We use three types of control algorithms for the pressure control: classical PID control, feedback linearization control, and fuzzy PID control, and two control algorithms for thrust control: classical PID control and fuzzy PID control. Finally, we compare the performance of each control system through a numerical simulation using step responses. Through this work, we check that feedback linearization is better in pressure control, and fuzzy PID control is more appropriate in thrust control. Especially using fuzzy PID control, we can get fast settling with a small undershoot even if the system is a nonminimum phase system.
Sex and Body Colour Affect the Variation in Internal Body Temperature of Oedaleus decorus asiaticus in Natural Habitats in Inner Mongolia, China
Authors: Cheng Yumeng , Li Hongmei , Liu Lulu , Wang Guangjun , Gu Haojing , Luke Belinda
Published: June 2022

Oedaleus decorus asiaticus is one of the most harmful locusts in agricultural and pastoral areas in China. Plagues of this grasshopper can aggravate grassland degradation and cause huge damage to the livestock industry. Fungal biopesticides are seen as a suitable means of controlling grasshoppers and locusts. However, the efficiency of fungal biopesticides is dependent on temperature. Currently, there is limited knowledge on the thermal biology of this grasshopper in natural habitats. In this study, ground temperature measurements were made in conjunction with measurements of internal body temperatures using thermocouples and hand-held thermometers. The grasshoppers were randomly caught during the daytime in 2017 and 2018 in eight different locations in Inner Mongolia Autonomous Region, China. Our results indicated that the average internal body temperature of nymphs as well as adults of O. d. asiaticusis was higher than the ground temperature and that it increases/decreases with increases/decreases in ground temperature, respectively, during the daytime. Moreover, the adult internal body temperature is significantly higher than that of the nymphs at different times of the day, specifically around 6:00, 10:00, 13:00, and 18:00. Female internal body temperatures were significantly higher than those of the males by an average of 0.90 °C. Additionally, the average internal body temperature of the brown morphs was higher than that of the green morphs by approximately 1.17 °C. These findings demonstrate that brown morph insects might be more tolerant of fungal biopesticides and hence the biopesticides may take longer to kill them. Hence, ecophysiological adaptations to climate change may affect how fungal biopesticides could be used in the future.
Evaluation of Agricultural Value of Composts Prepared from Municipal Biowastes in Different Conditions of Composting Process
Authors: Jakubus Monika , Spychalski Waldemar
Published: June 2022

The increasing mass of organic waste as well as the assumptions of a circular economy enforce the rational management of this type of waste. One method of recycling is composting, which makes it possible to use waste efficiently as an organic fertilizer. This paper presents the results of a comparative study of six different composts in terms of their agricultural quality. The aim of this study was to evaluate the bioavailable amounts of metals using single extractions with DTPA solution and to characterize various humic compounds. Particular attention was paid to the amounts of labile carbon (LC), hot water-extractable carbon (HWC), and the quantity and quality of humus substances (HS). Regardless of compost types, they were characterized by a small share of easily decomposable compounds such as fulvic acids (FAs), LC, and HWC, which may indicate the low susceptibility of compost humic substances to microbiological degradation in soil. In general, the bioavailable metal amounts found in the analyzed composts were low; therefore, the tested composts applied to the soil can be considered safe for the environment.
Short- and Medium-Term Effects of On-Farm Compost Addition on the Physical and Hydraulic Properties of a Clay Soil
Authors: Castellini Mirko , Diacono Mariangela , Preite Antonio , Montemurro Francesco
Published: June 2022

Compost amendment is a widespread agronomic practice, but little information is available about the short- and medium-term effects on clay soils. In this investigation, we selected three soil compost rates (treatments, T), i.e., fertilizer (T2 = 1.5 kg m−2), amending (T3 = 15 kg m−2), and organic (T4 = 75 kg m−2), that were compared to a control (T1). Our research accounts for the effects reached on representative large boxes (about 0.75 m3), without crops and for about eighteen months. An overall assessment of the physical and hydraulic properties of the soil was made, including infiltration rate or saturated hydraulic conductivity (Ks), water content and water retention (θ) of the soil, bulk density and structure, and several physical quality indicators obtained from the water retention curve, accounting for the optimal balance between water/air into the soil, pore volume distribution function, and soil features in the inflection point of the soil water retention curve. Additionally, the temporal changes of Ks were evaluated. The main results showed that (i) after eighteen months, and regardless of T, θ significantly improved by a factor of 1.2–1.3, but these improvements (up to 1%) were detected only close to water saturation (i.e., until 6 cm of soil pressure head) when little (T2) or no compost (T1) addition was considered, while a larger range was detected (until 60 cm) when higher rates (T3–T4) were used; (ii) Ks determination allowed to establish that compost effects vanished after about eleven months, but it was not possible to verify that composting increased the permeability of the investigated clay soil within that time frame; (iii) some significant correlations between Ks and some soil physical indicators estimated from both the inflection point of the water retention curve or bulk density suggested possible improvements in soil permeability. Because some factors (water content of the soil above all) could have affected the comparison of Ks measurements, further research on this topic is needed.
Comparative Genetic Diversity Analysis for Biomass Allocation and Drought Tolerance in Wheat
Authors: Shamuyarira Kwame W. , Shimelis Hussein , Mathew Isack , Shayanowako Admire , Zengeni Rebecca , Chaplot Vincent
Published: June 2022

Genetic diversity is invaluable in developing climate-smart and drought-adapted wheat varieties. The aim of this study was to determine the extent of genetic variation present in wheat germplasm collections for biomass allocation and drought tolerance based on complementary phenotypic and root attributes and high-density single nucleotide polymorphism (SNP) markers to select breeding parents. A total of 97 bread wheat (Triticum aestivum L.) genotypes were evaluated in field and greenhouse trials under drought-stressed and non-stressed conditions. The molecular variance analysis showed that the intrapopulation variance was very high at 99%, with a small minimal inter-population variance (1%). The genetic distance, polymorphic information content and expected heterozygosity were 0.20–0.88, 0.24–1.00 and 0.29–0.58, respectively. The cluster analysis based on SNP data showed that 44% and 28% of the assessed genotypes maintained their genetic groups when compared to hierarchical clusters under drought-stressed and non-stressed phenotypic data, respectively. The joint analysis using genotypic and phenotypic data resolved three heterotic groups and allowed the selection of genotypes BW140, BW152, BW157, BW162, LM30, LM47, LM48, LM52, LM54 and LM70. The selected genotypes were the most genetically divergent with high root biomass and grain yield and are recommended for production or breeding.
A Simple and Reliable Liquid Chromatographic Method for Simultaneous Determination of Five Benzodiazepine Drugs in Human Plasma
Authors: Albishri Hassan M. , Aldawsari Naflaa A. , Abd El-Hady Deia
Published: June 2022

Benzodiazepines (BZDs) are one of the most important drugs that have been used in the treatment of neuropsychological disorders. Indeed, BZDs are abused by drug addicts regardless of their therapeutic uses. Therefore, it was important in forensic and clinical toxicology to reach an easy and reliable method for the screening and quantification of BZDs in the human plasma matrix. In the current work, five BZDs, namely bromazepam, clonazepam, lorazepam, nordiazepam and diazepam were simultaneously separated and detected by a simple and reliable RPLC method in a human plasma matrix. Isocratic mobile elution consisting of 20 mmol L−1 phosphate buffer (pH 7.0) and methanol (50:50, v/v) on a Symmetry C18 column was employed. The flow rate, wavelength and column temperature were fixed at 1.0 mL min−1, 214 nm and 40 °C, respectively. The proposed method was validated, giving a linearity within the concentration ranges 5–500 ng mL−1 for bromazepam and diazepam, 3–500 ng mL−1 for clonazepam and lorazepam and 1–500 ng mL−1 for nordiazepam with a determination coefficient (R2) more than 0.9992. The LOD values for the selected BZDs ranged from 0.54 to 2.32 and from 1.78 to 7.65 ng mL−1 for standard methanolic and plasma matrices, respectively. Precision, accuracy, selectivity, stability, and robustness were some of the terms considered in validating the current RPLC method. Based on these results, a simple and reliable RPLC method was successfully applied to quantify BZDs in human plasma matrix appearing with recoveries ranging from 96.5 to 107.5% and interday RSD less than 4%. The current developed method was useful for rapidly screening the most commonly used BZDs in the market within their therapeutic concentration ranges.
Prevalence and Correlates of Self-Medication Practices for Prevention and Treatment of COVID-19: A Systematic Review
Authors: Ayosanmi Oluwasola Stephen , Alli Babatunde Yusuf , Akingbule Oluwatosin Adetolani , Alaga Adeyemi Hakeem , Perepelkin Jason , Marjorie Delbaere , Sansgiry Sujit S. , Taylor Jeffrey
Published: June 2022

It has been suggested that the COVID-19 pandemic led to an increase in self-medication practices across the world. Yet, there is no up-to-date synthesized evidence on the prevalence of self-medication that is attributable to the pandemic. This study aimed to conduct a systematic literature review on the prevalence and correlates of self-medication for the prevention and treatment of COVID-19 globally. The review was registered with the PROSPERO database. Searches were conducted following PRISMA guidelines, and relevant articles published between 1 April 2020 and 31 March 2022 were included. Pooled prevalence rate was conducted using the Meta package in R. A total of 14 studies from 14 countries, which represented 15,154 participants, were included. The prevalence of COVID-19-related self-medication ranged from 3.4–96%. The pooled prevalence of self-medication for this purpose was 44.9% (95% CI: 23.8%, 68.1%). Medications reported by studies for self-medication were antibiotics (79%), vitamins (64%), antimalarials (50%), herbal and natural products (50%), analgesics and antipyretics (43%), minerals and supplements (43%), cold and allergy preparations (29%), corticosteroids (14%), and antivirals (7%). The prevalence of self-medication with antibiotics is concerning. More public health education about responsible self-medication amidst the COVID-19 pandemic and future pandemics is required to mitigate the rising threat of antimicrobial resistance.
Effect of the Symbiosis with Mycoplasma hominis and Candidatus Mycoplasma Girerdii on Trichomonas vaginalis Metronidazole Susceptibility
Authors: Margarita Valentina , Cao Le Chi , Bailey Nicholas P. , Ngoc Thuy Ha Thi , Ngo Thi Minh Chau , Nu Phuong Anh Ton , Diaz Nicia , Dessì Daniele , Hirt Robert P. , Fiori Pier Luigi , Rappelli Paola
Published: June 2022

Trichomoniasis, the most common non-viral sexually transmitted infection worldwide, is caused by the protozoon Trichomonas vaginalis. The 5- nitroimidazole drugs, of which metronidazole is the most prescribed, are the only effective drugs to treat trichomoniasis. Resistance against metronidazole is increasingly reported among T. vaginalis isolates. T. vaginalis can establish an endosymbiosis with two Mycoplasma species, Mycoplasma hominis and Candidatus Mycoplasma girerdii, whose presence has been demonstrated to influence several aspects of the protozoan pathobiology. The role of M. hominis in T. vaginalis resistance to metronidazole is controversial, while the influence of Ca. M. girerdii has never been investigated. In this work, we investigate the possible correlation between the presence of Ca. M. girerdii and/or M. hominis and the in vitro drug susceptibility in a large group of T. vaginalis isolated in Italy and in Vietnam. We also evaluated, via RNA-seq analysis, the expression of protozoan genes involved in metronidazole resistance in a set of syngenic T. vaginalis strains, differing only for the presence/absence of the two Mycoplasmas. Our results show that the presence of M. hominis significantly increases the sensitivity to metronidazole in T. vaginalis and affects gene expression. On the contrary, the symbiosis with Candidatus Mycoplasma girerdii seems to have no effect on metronidazole resistance in T. vaginalis.
Editorial for the Special Issue: “Therapeutic Drug Monitoring of Antimicrobials”
Authors: Gijsen Matthias , Allegaert Karel
Published: June 2022

A recent guideline [...]
Ethyl Acetate Fraction of Bixa orellana and Its Component Ellagic Acid Exert Antibacterial and Anti-Inflammatory Properties against Mycobacterium abscessus subsp. massiliense
Authors: Moraes-Neto Roberval Nascimento , Coutinho Gabrielle Guedes , Ataíde Ana Caroline Santos , de Oliveira Rezende Aline , Nascimento Camila Evangelista Carnib , de Albuquerque Rafaela Pontes , da Rocha Cláudia Quintino , Rêgo Adriana Sousa , de Sousa Cartágenes Maria do Socorro , Abreu-Silva Ana Lúcia , dos Santos Igor Victor Ferreira , dos Santos Cleydson Breno Rodrigues , Guerra Rosane Nassar Meireles , Ribeiro Rachel Melo , Monteiro-Neto Valério , de Sousa Eduardo Martins , Carvalho Rafael Cardoso
Published: June 2022

Mycobacterium abscessus subsp. massiliense (Mabs) causes chronic infections, which has led to the need for new antimycobacterial agents. In this study, we investigated the antimycobacterial and anti-inflammatory activities of the ethyl acetate fraction of Bixa orellana leaves (BoEA) and ellagic acid (ElAc). In silico analysis predicted that ElAc had low toxicity, was not mutagenic or carcinogenic, and had antimicrobial and anti-inflammatory activities. Apparently, ElAc can interact with COX2 and Dihydrofolate reductase (DHFR) enzymes, which could explain both activities. In vitro analysis showed that BoEA and ElAc exerted antimicrobial activity against Mabs (minimum inhibitory concentration of 1.56, 1.56 mg/mL and bactericidal concentration of 6.25, 3.12 mg/mL, respectively. Clarithromycin showed MIC and MBC of 1 and 6 µg/mL). Treatment with BoEA or ElAc increased survival of Tenebrio molitor larvae after lethal infection with Mabs and reduced carrageenan-induced paw edema in mice, around 40% of edema volume after the fourth hour, similarly to diclofenac. In conclusion, BoEA and ElAc exert antimicrobial effects against Mabs and have anti-inflammatory effects, making them potential sources of antimycobacterial drugs. The biological activities of ElAc may be due to its high binding affinities predicted for COX2 and DHFR enzymes.