bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Varying Number of Radio Occultation Observations on Regional Weather Prediction Over India During the Summer Monsoon Season
Authors: Singh Randhir , Ojha Satya P. , Anthes Richard
Published: July 2024

Observing system simulation experiments are carried out to investigate the added value of radio occultation (RO) refractivity observations with various spatial sampling scenarios on regional weather predictions across the Indian region. A full summer monsoon season (June through September 2020) was used to demonstrate how varying the numbers and horizontal resolutions of RO data impacted regional‐scale weather forecasting. The MPAS (Model for Prediction Across Scales) was used to produce a nature run at a maximum horizontal resolution of 10 km. Then the WRF model with 12‐km horizontal resolution was used to carry out assimilation/forecast experiments with varying number of simulated RO observations. When the performance of the experiments is taken into account for moisture, temperature, winds and rainfall, as well as prediction lengths, the results show that RO observations with 50‐km resolution assimilated every 6 hr would provide the best results. Increasing the horizontal resolution to 25 km per 6 hr shows little overall improvement. Furthermore, RO data with horizontal resolutions lower than 100 km per 6 hr have only a small impact on the regional numerical weather prediction system. The number of low Earth orbit satellites in low‐inclination orbits required to achieve occultations every 6 hr with 50‐km resolution based on the COSMIC‐2 mission is approximately 700. This work is relevant for the deployment of the cost‐effective RO observing system for improved weather forecasting over the Indian region.
Architecture design and advanced manufacturing of heart-on-a-chip: scaffolds, stimulation and sensors
Authors: Xu Feng , Jin Hang , Liu Lingling , Yang Yuanyuan , Cen Jianzheng , Wu Yaobin , Chen Songyue , Sun Daoheng
Published: July 2024

Heart-on-a-chip (HoC) has emerged as a highly efficient, cost-effective device for the development of engineered cardiac tissue, facilitating high-throughput testing in drug development and clinical treatment. HoC is primarily used to create a biomimetic microphysiological environment conducive to fostering the maturation of cardiac tissue and to gather information regarding the real-time condition of cardiac tissue. The development of architectural design and advanced manufacturing for these “3S” components, scaffolds, stimulation, and sensors is essential for improving the maturity of cardiac tissue cultivated on-chip, as well as the precision and accuracy of tissue states. In this review, the typical structures and manufacturing technologies of the “3S” components are summarized. The design and manufacturing suggestions for each component are proposed. Furthermore, key challenges and future perspectives of HoC platforms with integrated “3S” components are discussed.
YTH domain family protein 3 accelerates non-small cell lung cancer immune evasion through targeting CD8+ T lymphocytes
Authors: Luo Yisheng , Zeng Chao , Ouyang Zezhong , Zhu Wenbin , Wang Jiazhi , Chen Zhiyin , Xiao Chunyang , Wu Guodong , Li Liang , Qian Youhui , Chen Xin , Liu Yuchen , Wu Hao
Published: July 2024

Immune evasion is one of the critical hallmarks of malignant tumors, especially non-small cell lung cancer (NSCLC). Emerging findings have illustrated the roles of N6-methyladenosine (m6A) on NSCLC immune evasion. Here, this study investigated the function and underlying mechanism of m6A reader YTH domain family protein 3 (YTHDF3) on NSCLC immune evasion. YTHDF3 was found to be highly expressed in NSCLC tissue and act as an independent prognostic factor for overall survival. Functionally, up-regulation of YTHDF3 impaired the CD8+ T antitumor activity to deteriorate NSCLC immune evasion, while YTHDF3 silencing recovered the CD8+ T antitumor activity to inhibit immune evasion. Besides, YTHDF3 up-regulation reduced the apoptosis of NSCLC cells. Mechanistically, PD-L1 acted as the downstream target for YTHDF3, and YTHDF3 could upregulate the transcription stability of PD-L1 mRNA. Overall, YTHDF3 targeted PD-L1 to promote NSCLC immune evasion partially through escaping effector cell cytotoxicity CD8+ T mediated killing and antitumor immunity. In summary, this study provides an essential insight for m6A modification on CD8+ T cell-mediated antitumor immunity in NSCLC, which might inspire an innovation for lung cancer tumor immunotherapy.
GolpHCat (TMEM87A), a unique voltage-dependent cation channel in Golgi apparatus, contributes to Golgi-pH maintenance and hippocampus-dependent memory
Authors: Kang Hyunji , Han Ah-reum , Zhang Aihua , Jeong Heejin , Koh Wuhyun , Lee Jung Moo , Lee Hayeon , Jo Hee Young , Maria-Solano Miguel A. , Bhalla Mridula , Kwon Jea , Roh Woo Suk , Yang Jimin , An Hyun Joo , Choi Sun , Kim Ho Min , Lee C. Justin
Published: July 2024

Impaired ion channels regulating Golgi pH lead to structural alterations in the Golgi apparatus, such as fragmentation, which is found, along with cognitive impairment, in Alzheimer’s disease. However, the causal relationship between altered Golgi structure and cognitive impairment remains elusive due to the lack of understanding of ion channels in the Golgi apparatus of brain cells. Here, we identify that a transmembrane protein TMEM87A, renamed Golgi-pH-regulating cation channel (GolpHCat), expressed in astrocytes and neurons that contributes to hippocampus-dependent memory. We find that GolpHCat displays unique voltage-dependent currents, which is potently inhibited by gluconate. Additionally, we gain structural insights into the ion conduction through GolpHCat at the molecular level by determining three high-resolution cryogenic-electron microscopy structures of human GolpHCat. GolpHCat-knockout mice show fragmented Golgi morphology and altered protein glycosylation and functions in the hippocampus, leading to impaired spatial memory. These findings suggest a molecular target for Golgi-related diseases and cognitive impairment.
A scalable and cGMP-compatible autologous organotypic cell therapy for Dystrophic Epidermolysis Bullosa
Authors: Neumayer Gernot , Torkelson Jessica L. , Li Shengdi , McCarthy Kelly , Zhen Hanson H. , Vangipuram Madhuri , Mader Marius M. , Gebeyehu Gulilat , Jaouni Taysir M. , Jacków-Malinowska Joanna , Rami Avina , Hansen Corey , Guo Zongyou , Gaddam Sadhana , Tate Keri M. , Pappalardo Alberto , Li Lingjie , Chow Grace M. , Roy Kevin R. , Nguyen Thuylinh Michelle , Tanabe Koji , McGrath Patrick S. , Cramer Amber , Bruckner Anna , Bilousova Ganna , Roop Dennis , Tang Jean Y. , Christiano Angela , Steinmetz Lars M. , Wernig Marius , Oro Anthony E.
Published: July 2024

We present Dystrophic Epidermolysis Bullosa Cell Therapy (DEBCT), a scalable platform producing autologous organotypic iPS cell-derived induced skin composite (iSC) grafts for definitive treatment. Clinical-grade manufacturing integrates CRISPR-mediated genetic correction with reprogramming into one step, accelerating derivation of COL7A1-edited iPS cells from patients. Differentiation into epidermal, dermal and melanocyte progenitors is followed by CD49f-enrichment, minimizing maturation heterogeneity. Mouse xenografting of iSCs from four patients with different mutations demonstrates disease modifying activity at 1 month. Next-generation sequencing, biodistribution and tumorigenicity assays establish a favorable safety profile at 1-9 months. Single cell transcriptomics reveals that iSCs are composed of the major skin cell lineages and include prominent holoclone stem cell-like signatures of keratinocytes, and the recently described Gibbin-dependent signature of fibroblasts. The latter correlates with enhanced graftability of iSCs. In conclusion, DEBCT overcomes manufacturing and safety roadblocks and establishes a reproducible, safe, and cGMP-compatible therapeutic approach to heal lesions of DEB patients.
Aperiodic approximants bridging quasicrystals and modulated structures
Authors: Matsubara Toranosuke , Koga Akihisa , Takano Atsushi , Matsushita Yushu , Dotera Tomonari
Published: July 2024

Aperiodic crystals constitute a class of materials that includes incommensurate (IC) modulated structures and quasicrystals (QCs). Although these two categories share a common foundation in the concept of superspace, the relationship between them has remained enigmatic and largely unexplored. Here, we show “any metallic-mean” QCs, surpassing the confines of Penrose-like structures, and explore their connection with IC modulated structures. In contrast to periodic approximants of QCs, our work introduces the pivotal role of “aperiodic approximants”, articulated through a series of k-th metallic-mean tilings serving as aperiodic approximants for the honeycomb crystal, while simultaneously redefining this tiling as a metallic-mean IC modulated structure, highlighting the intricate interplay between these crystallographic phenomena. We extend our findings to real-world applications, discovering these tiles in a terpolymer/homopolymer blend and applying our QC theory to a colloidal simulation displaying planar IC structures. In these structures, domain walls are viewed as essential components of a quasicrystal, introducing additional dimensions in superspace. Our research provides a fresh perspective on the intricate world of aperiodic crystals, shedding light on their broader implications for domain wall structures across various fields.
Hyperconfined bio-inspired Polymers in Integrative Flow-Through Systems for Highly Selective Removal of Heavy Metal Ions
Authors: Nakahata Masaki , Sumiya Ai , Ikemoto Yuka , Nakamura Takashi , Dudin Anastasia , Schwieger Julius , Yamamoto Akihisa , Sakai Shinji , Kaufmann Stefan , Tanaka Motomu
Published: July 2024

Access to clean water, hygiene, and sanitation is becoming an increasingly pressing global demand, particularly owing to rapid population growth and urbanization. Phytoremediation utilizes a highly conserved phytochelatin in plants, which captures hazardous heavy metal ions from aquatic environments and sequesters them in vacuoles. Herein, we report the design of phytochelatin-inspired copolymers containing carboxylate and thiolate moieties. Titration calorimetry results indicate that the coexistence of both moieties is essential for the excellent Cd2+ ion-capturing capacity of the copolymers. The obtained dissociation constant, KD ~ 1 nM for Cd2+ ion, is four-to-five orders of magnitude higher than that for peptides mimicking the sequence of endogenous phytochelatin. Furthermore, infrared and nuclear magnetic resonance spectroscopy results unravel the mechanism underlying complex formation at the molecular level. The grafting of 0.1 g bio-inspired copolymers onto silica microparticles and cellulose membranes helps concentrate the copolymer-coated microparticles in ≈3 mL volume to remove Cd2+ ions from 0.3 L of water within 1 h to the drinking water level (<0.03 µM). The obtained results suggest that hyperconfinement of bio-inspired polymers in flow-through systems can be applied for the highly selective removal of harmful contaminants from the environmental water.
Tumor cell-directed STING agonist antibody-drug conjugates induce type III interferons and anti-tumor innate immune responses
Authors: Malli Cetinbas Naniye , Monnell Travis , Soomer-James Jahna , Shaw Pamela , Lancaster Kelly , Catcott Kalli C. , Dolan Melissa , Mosher Rebecca , Routhier Caitlin , Chin Chen-Ni , Toader Dorin , Duvall Jeremy , Bukhalid Raghida , Lowinger Timothy B. , Damelin Marc
Published: July 2024

Activating interferon responses with STING agonists (STINGa) is a current cancer immunotherapy strategy, and therapeutic modalities that enable tumor-targeted delivery via systemic administration could be beneficial. Here we demonstrate that tumor cell-directed STING agonist antibody-drug-conjugates (STINGa ADCs) activate STING in tumor cells and myeloid cells and induce anti-tumor innate immune responses in in vitro, in vivo (in female mice), and ex vivo tumor models. We show that the tumor cell-directed STINGa ADCs are internalized into myeloid cells by Fcγ-receptor-I in a tumor antigen-dependent manner. Systemic administration of STINGa ADCs in mice leads to STING activation in tumors, with increased anti-tumor activity and reduced serum cytokine elevations compared to a free STING agonist. Furthermore, STINGa ADCs induce type III interferons, which contribute to the anti-tumor activity by upregulating type I interferon and other key chemokines/cytokines. These findings reveal an important role for type III interferons in the anti-tumor activity elicited by STING agonism and provide rationale for the clinical development of tumor cell-directed STINGa ADCs.
Molecular profiling of 888 pediatric tumors informs future precision trials and data-sharing initiatives in pediatric cancer
Authors: Forrest Suzanne J. , Gupta Hersh , Ward Abigail , Li Yvonne Y. , Doan Duong , Al-Ibraheemi Alyaa , Alexandrescu Sanda , Bandopadhayay Pratiti , Shusterman Suzanne , Mullen Elizabeth A. , Collins Natalie B. , Chi Susan N. , Wright Karen D. , Kumari Priti , Mazor Tali , Ligon Keith L. , Shivdasani Priyanka , Manam Monica , MacConaill Laura E. , Ceca Evelina , Benich Sidney N. , London Wendy B. , Schilsky Richard L. , Bruinooge Suanna S. , Guidry Auvil Jaime M. , Cerami Ethan , Rollins Barrett J. , Meyerson Matthew L. , Lindeman Neal I. , Johnson Bruce E. , Cherniack Andrew D. , Church Alanna J. , Janeway Katherine A.
Published: July 2024

To inform clinical trial design and real-world precision pediatric oncology practice, we classified diagnoses, assessed the landscape of mutations, and identified genomic variants matching trials in a large unselected institutional cohort of solid tumors patients sequenced at Dana-Farber / Boston Children’s Cancer and Blood Disorders Center. Tumors were sequenced with OncoPanel, a targeted next-generation DNA sequencing panel. Diagnoses were classified according to the International Classification of Diseases for Oncology (ICD-O-3.2). Over 6.5 years, 888 pediatric cancer patients with 95 distinct diagnoses had successful tumor sequencing. Overall, 33% (n = 289/888) of patients had at least 1 variant matching a precision oncology trial protocol, and 14% (41/289) were treated with molecularly targeted therapy. This study highlights opportunities to use genomic data from hospital-based sequencing performed either for research or clinical care to inform ongoing and future precision oncology clinical trials. Furthermore, the study results emphasize the importance of data sharing to define the genomic landscape and targeted treatment opportunities for the large group of rare pediatric cancers we encounter in clinical practice.
Organocatalytic asymmetric synthesis of Si-stereogenic silacycles
Authors: Han Jung Tae , Tsuji Nobuya , Zhou Hui , Leutzsch Markus , List Benjamin
Published: July 2024

A strong and confined Brønsted acid catalyzed enantioselective cyclization of bis(methallyl)silanes provides enantioenriched Si-stereogenic silacycles. High enantioselectivities of up to 96.5:3.5 er were obtained for a range of bis(methallyl)silanes. NMR and ESI-MS studies reveal that the formation of a covalent adduct irreversibly inhibits turnover. Remarkably, we found that acetic acid as an additive promotes the collapse of this adduct, enabling full turnover. Experimental investigation and density functional theory (DFT) calculations were conducted to elucidate the origin of this phenomenon and the observed enantioselectivity.
Harnessing virus flexibility to selectively capture and profile rare circulating target cells for precise cancer subtyping
Authors: Li Hui-Da , Chen Yuan-Qiang , Li Yan , Wei Xing , Wang Si-Yi , Cao Ying , Wang Rui , Wang Cong , Li Jing-Yue , Li Jian-Yi , Ding Hong-Ming , Yang Ting , Wang Jian-Hua , Mao Chuanbin
Published: July 2024

The effective isolation of rare target cells, such as circulating tumor cells, from whole blood is still challenging due to the lack of a capturing surface with strong target-binding affinity and non-target-cell resistance. Here we present a solution leveraging the flexibility of bacterial virus (phage) nanofibers with their sidewalls displaying target circulating tumor cell-specific aptamers and their ends tethered to magnetic beads. Such flexible phages, with low stiffness and Young’s modulus, can twist and adapt to recognize the cell receptors, energetically enhancing target cell capturing and entropically discouraging non-target cells (white blood cells) adsorption. The magnetic beads with flexible phages can isolate and count target cells with significant increase in cell affinity and reduction in non-target cell absorption compared to magnetic beads having rigid phages. This differentiates breast cancer patients and healthy donors, with impressive area under the curve (0.991) at the optimal detection threshold (>4 target cells mL−1). Immunostaining of captured circulating tumor cells precisely determines breast cancer subtypes with a diagnostic accuracy of 91.07%. Our study reveals the power of viral mechanical attributes in designing surfaces with superior target binding and non-target anti-fouling.
Atomic sawtooth-like metal films for vdW-layered single-crystal growth
Authors: Ko Hayoung , Choi Soo Ho , Park Yunjae , Lee Seungjin , Oh Chang Seok , Kim Sung Youb , Lee Young Hee , Kim Soo Min , Ding Feng , Kim Ki Kang
Published: July 2024

Atomic sawtooth surfaces have emerged as a versatile platform for growth of single-crystal van der Waals layered materials. However, the mechanism governing the formation of single-crystal atomic sawtooth metal (copper or gold) films on hard substrates (tungsten or molybdenum) remains a puzzle. In this study, we aim to elucidate the formation mechanism of atomic sawtooth metal films during melting–solidification process. Utilizing molecular dynamics, we unveil that the solidification of the liquid copper initiates at a high-index tungsten facet with higher interfacial energy. Subsequent tungsten facets follow energetically favourable pathways of forming single-crystal atomic sawtooth copper film during the solidification process near melting temperature. Formation of atomic sawtooth copper film is guaranteed with a film thickness exceeding the grain size of polycrystalline tungsten substrate. We further demonstrate the successful growth of centimeter-scale single-crystal monolayer hexagonal boron nitride films on atomic sawtooth copper films and explore their potential as efficient oxygen barrier.
White-light activatable organic NIR-II luminescence nanomaterials for imaging-guided surgery
Authors: Li Chunbin , Du Jian , Jiang Guoyu , Gong Jianye , Zhang Yue , Yao Mengfan , Wang Jianguo , Wu Limin , Tang Ben Zhong
Published: July 2024

While second near-infrared (NIR-II) fluorescence imaging is a promising tool for real-time surveillance of surgical operations, the previously reported organic NIR-II luminescent materials for in vivo imaging are predominantly activated by expensive lasers or X-ray with high power and poor illumination homogeneity, which significantly limits their clinical applications. Here we report a white-light activatable NIR-II organic imaging agent by taking advantages of the strong intramolecular/intermolecular D-A interactions of conjugated Y6CT molecules in nanoparticles (Y6CT-NPs), with the brightness of as high as 13315.1, which is over two times that of the brightest laser-activated NIR-II organic contrast agents reported thus far. Upon white-light activation, Y6CT-NPs can achieve not only in vivo imaging of hepatic ischemia reperfusion, but also real-time monitoring of kidney transplantation surgery. During the surgery, identification of the renal vasculature, post-reconstruction assessment of renal allograft vascular integrity, and blood supply analysis of the ureter can be vividly depicted by using Y6CT-NPs with high signal-to-noise ratios upon clinical laparoscopic LED white-light activation. Our work provides efficient molecular design guidelines towards white-light activatable imaging agent and highlights an opportunity for precision imaging theranostics.
Spontaneous symmetry breaking in polar fluids
Authors: Gibb Calum J. , Hobbs Jordan , Nikolova Diana I. , Raistrick Thomas , Berrow Stuart R. , Mertelj Alenka , Osterman Natan , Sebastián Nerea , Gleeson Helen F. , Mandle Richard. J.
Published: July 2024

Spontaneous symmetry breaking and emergent polar order are each of fundamental importance to a range of scientific disciplines, as well as generating rich phase behaviour in liquid crystals (LCs). Here, we show the union of these phenomena to lead to two previously undiscovered polar liquid states of matter. Both phases have a lamellar structure with an inherent polar ordering of their constituent molecules. The first of these phases is characterised by polar order and a local tilted structure; the tilt direction processes about a helix orthogonal to the layer normal, the period of which is such that we observe selective reflection of light. The second new phase type is anti-ferroelectric, with the constituent molecules aligning orthogonally to the layer normal. This has led us to term the phases the $${{{{{\rm{Sm}}}}}}{{{{{{\rm{C}}}}}}}_{{{{{{\rm{P}}}}}}}^{{{{{{\rm{H}}}}}}}$$
Sm

C

P

H

and SmAAF phases, respectively. Further to this, we obtain room temperature ferroelectric nematic (NF) and $${{{{{\rm{Sm}}}}}}{{{{{{\rm{C}}}}}}}_{{{{{{\rm{P}}}}}}}^{{{{{{\rm{H}}}}}}}$$
Sm

C

P

H

phases via binary mixture formulation of the novel materials described here with a standard NF compound (DIO), with the resultant materials having melting points (and/or glass transitions) which are significantly below ambient temperature. The new soft matter phase types discovered herein can be considered as electrical analogues of topological structures of magnetic spins in hard matter.
Endonucleosis mediates internalization of cytoplasm into the nucleus
Authors: Galanopoulou Ourania , Tachmatzidi Evangelia C. , Deligianni Elena , Botskaris Dimitris , Nikolaou Kostas C. , Gargani Sofia , Dalezios Yannis , Chalepakis Georges , Talianidis Iannis
Published: July 2024

Setd8 regulates transcription elongation, mitotic DNA condensation, DNA damage response and replication licensing. Here we show that, in mitogen-stimulated liver-specific Setd8-KO mice, most of the hepatocytes are eliminated by necrosis but a significant number of them survive via entering a stage exhibiting several senescence-related features. Setd8-deficient hepatocytes had enlarged nuclei, chromosomal hyperploidy and nuclear engulfments progressing to the formation of intranuclear vesicles surrounded by nuclear lamina. These vesicles contain glycogen, cytoplasmic proteins and even entire organelles. We term this process “endonucleosis”. Intranuclear vesicles are absent in hepatocytes of Setd8/Atg5 knockout mice, suggesting that the process requires the function of the canonical autophagy machinery. Endonucleosis and hyperploidization are temporary, early events in the surviving Setd8-deficient cells. Larger vesicles break down into microvesicles over time and are eventually eliminated. The results reveal sequential events in cells with extensive DNA damage, which function as part of survival mechanisms to prevent necrotic death.
Perturbed N-glycosylation of Halobacterium salinarum archaellum filaments leads to filament bundling and compromised cell motility
Authors: Sofer Shahar , Vershinin Zlata , Mashni Leen , Zalk Ran , Shahar Anat , Eichler Jerry , Grossman-Haham Iris
Published: July 2024

The swimming device of archaea—the archaellum—presents asparagine (N)-linked glycans. While N-glycosylation serves numerous roles in archaea, including enabling their survival in extreme environments, how this post-translational modification contributes to cell motility remains under-explored. Here, we report the cryo-EM structure of archaellum filaments from the haloarchaeon Halobacterium salinarum, where archaellins, the building blocks of the archaellum, are N-glycosylated, and the N-glycosylation pathway is well-resolved. We further determined structures of archaellum filaments from two N-glycosylation mutant strains that generate truncated glycans and analyzed their motility. While cells from the parent strain exhibited unidirectional motility, the N-glycosylation mutant strain cells swam in ever-changing directions within a limited area. Although these mutant strain cells presented archaellum filaments that were highly similar in architecture to those of the parent strain, N-linked glycan truncation greatly affected interactions between archaellum filaments, leading to dramatic clustering of both isolated and cell-attached filaments. We propose that the N-linked tetrasaccharides decorating archaellins act as physical spacers that minimize the archaellum filament aggregation that limits cell motility.
Polyepitaxial grain matching to study the oxidation of uranium dioxide
Authors: Wasik Jacek , Sutcliffe Joseph , Podor Renaud , Lewis Jarrod , Darnbrough James Edward , Rennie Sophie , Akbar Hussain Syed , Bell Christopher , Chaney Daniel Alexander , Griffiths Gareth , Harding Lottie Mae , Legg Florence , Lawrence Bright Eleanor , Nicholls Rebecca , Sasikumar Yadukrishnan , Siberry Angus , Smith Philip , Springell Ross
Published: July 2024

Although the principal physical behaviour of a material is inherently connected to its fundamental crystal structure, the behaviours observed in the real-world are often driven by the microstructure, which for many polycrystalline materials, equates to the size and shape of the constituent crystal grains. Here we highlight a cutting edge synthesis route to the controlled engineering of grain structures in thin films and the simplification of associated 3-dimensional problems to less complex 2D ones. This has been applied to the actinide ceramic, uranium dioxide, to replicate structures typical in nuclear fission fuel pellets, in order to investigate the oxidation and subsequent transformation of cubic UO2 to orthorhombic U3O8. This article shows how this synthesis approach could be utilised to investigate a range of phenomena, affected by grain morphology, and highlights some unusual results in the oxidation behaviour of UO2, regarding the phase transition to U3O8.
The role of ruminant urine and faeces in the recycling of nutrients by forages
Authors: Kao Pei-Tzu , McGrath Steve P. , Buss Heather L. , Darch Tegan , Warren Helen E. , McAuliffe Graham A. , Cardenas Laura , Blackwell Martin , Lee Michael R. F.
Published: July 2024

This study addresses the effect of using animal excreta on the nutritional content of forages, focusing on macro- and micro-element concentrations (nitrogen; N, phosphorus; P, sulphur; S, copper; Cu, zinc; Zn, manganese; Mn, selenium; Se) from animal feed to excreta, soil, and plants. Data were collected from pot and field trials using separate applications of sheep or cattle urine and faeces. Key findings indicate that soil organic carbon (SOC) and the type of excreta significantly influences nutrient uptake by forages, with varied responses among the seven elements defined above. Although urine contributes fewer micronutrients compared to faeces (as applied at a natural volume/mass basis, respectively), it notably improves forage yield and micronutrient accumulation, thus potentially delivering positive consequences at the farm level regarding economic performance and soil fertility when swards upon clayey soil types receive said urine in temperate agro-climatic regions (i.e., South West England in the current context). In contrast, faeces application in isolation hinders Se and Mn uptake, once again potentially delivering unintended consequences such as micronutrient deficiencies in areas of high faeces deposition. As it is unlikely that (b)ovine grazing fields will receive either urine or faeces in isolation, we also explored combined applications of both excreta types which demonstrates synergistic effects on N, Cu, and Zn uptake, with either synergistic or dilution effects being observed for P and S, depending largely on SOC levels. Additionally, interactions between excreta types can result in dilution or antagonistic effects on Mn and Se uptake. Notably, high SOC combined with faeces reduces Mn and Se in forages, raising concerns for grazed ruminant systems under certain biotic situations, e.g., due to insufficient soil Se levels typically observed in UK pastures for livestock growth. These findings underscore the importance of considering SOC and excreta nutritional composition when designing forage management to optimize nutrient uptake. It should be noted that these findings have potential ramifications for broader studies of sustainable agriculture through system-scale analyses, as the granularity of results reported herein elucidate gaps in knowledge which could affect, both positively and negatively, the interpretation of model-based environmental impact assessments of cattle and sheep production (e.g., in the case of increased yields [beneficial] or the requirement of additional synthetic supplementation [detrimental]).
Implicit bias and experience influence overall but not relative trustworthiness judgment of other-race faces
Authors: Cheung Olivia S. , Quimpo Nathan J. , Smoley James
Published: July 2024

Impressions of trustworthiness are formed quickly from faces. To what extent are these impressions shared among observers of the same or different races? Although high consensus of trustworthiness evaluation has been consistently reported, recent studies suggested substantial individual differences. For instance, negative implicit racial bias and low contact experience towards individuals of the other race have been shown to be related to low trustworthiness judgments for other-race faces. This pre-registered study further examined the effects of implicit social bias and experience on trustworthiness judgments of other-race faces. A relatively large sample of White (N = 338) and Black (N = 299) participants completed three tasks: a trustworthiness rating task of faces, a race implicit association test, and a questionnaire of experience. Each participant rated trustworthiness of 100 White faces and 100 Black faces. We found that the overall trustworthiness ratings for other-race faces were influenced by both implicit bias and experience with individuals of the other-race. Nonetheless, when comparing to the own-race baseline ratings, high correlations were observed for the relative differences in trustworthiness ratings of other-race faces for participants with varied levels of implicit bias and experience. These results suggest differential impact of social concepts (e.g., implicit bias, experience) vs. instinct (e.g., decision of approach-vs-avoid) on trustworthiness impressions, as revealed by overall vs. relative ratings on other-race faces.
Morphological classification of neurons based on Sugeno fuzzy integration and multi-classifier fusion
Authors: He Fuyun , Li Guanglian , Song Haixing
Published: July 2024

In order to extract more important morphological features of neuron images and achieve accurate classification of the neuron type, a method is proposed that uses Sugeno fuzzy integral integration of three optimized deep learning models, namely AlexNet, VGG11_bn, and ResNet-50. Firstly, using the pre-trained model of AlexNet and the output layer is fine-tuned to improve the model’s performance. Secondly, in the VGG11_bn network, Global Average Pooling (GAP) is adopted to replace the traditional fully connected layer to reduce the number of parameters. Additionally, the generalization ability of the model is improved by transfer learning. Thirdly, the SE(squeeze and excitation) module is added to the ResNet-50 variant ResNeXt-50 to adjust the channel weight and capture the key information of the input data. The GELU activation function is used to better fit the data distribution. Finally, Sugeno fuzzy integral is used to fuse the output of each model to get the final classification result. The experimental results showed that on the Img_raw, Img_resample and Img_XYalign dataset, the accuracy of 4-category classification reached 98.04%, 91.75% and 93.13%, respectively, and the accuracy of 12-category classification reached 97.82%, 85.68% and 87.60%, respectively. The proposed method has good classification performance in the morphological classification of neurons.
Exploring cell death mechanisms in spheroid cultures using a novel application of the RIP3-caspase3-assay
Authors: Philippi C. I. , Hagens J. , Heuer K. M. , Schmidt H. C. , Schuppert P. , Pagerols Raluy L. , Trochimiuk M. , Li Z. , Bunders M. J. , Reinshagen K. , Tomuschat C.
Published: July 2024

This study explores the application of the RIP3-caspase3-assay in heterogeneous spheroid cultures to analyze cell death pathways, emphasizing the nuanced roles of apoptosis and necroptosis. By employing directly conjugated monoclonal antibodies, we provide detailed insights into the complex mechanisms of cell death. Our findings demonstrate the assay’s capability to differentiate between RIP1-independent apoptosis, necroptosis, and RIP1-dependent apoptosis, marking a significant advancement in organoid research. Additionally, we investigate the effects of TNFα on isolated intestinal epithelial cells, revealing a concentration-dependent response and an adaptive or threshold reaction to TNFα-induced stress. The results indicate a preference for RIP1-independent cell death pathways upon TNFα stimulation, with a notable increase in apoptosis and a secondary role of necroptosis. Our research underscores the importance of the RIP3-caspase3-assay in understanding cell death mechanisms in organoid cultures, offering valuable insights for disease modeling and the development of targeted therapies. The assay’s adaptability and robustness in spheroid cultures enhances its potential as a tool in personalized medicine and translational research.
Preparation, characterization and antioxidant and anticancerous potential of Quercetin loaded β-glucan particles derived from mushroom and yeast
Authors: Trivedi Rashmi , Upadhyay Tarun Kumar
Published: July 2024

β-glucans are polysaccharides found in the cell walls of various fungi, bacteria and cereals. β-glucan have been found to show various kinds of anti-inflammatory, antimicrobial, antidiabetic antioxidant and anticancerous activities. In the present study, we have isolated β-glucan from the baker’s yeast Saccharomyces cerevisiae and white button mushroom Agaricus bisporus and tested their antioxidant potential and anticancerous activity against prostate cancer cell line PC3. Particles were characterized with zeta sizer and further with FTIR that confirmed that the isolated particles are β-glucan and alginate sealing made slow and sustained release of the Quercetin from the β-glucan particles. Morphological analysis of the hollow and Quercetin loaded β-glucan was performed with the SEM analysis and stability was analyzed with TGA and DSC analysis that showed the higher stability of the alginate sealed particles. Assessments of the antioxidant potential showed that Quercetin loaded particles were having higher antioxidant activity than hollow β-glucan particles. Cell viability of the PC3 cells was examined with MTT assay and it was found that Quercetin loaded alginate sealed Agaricus bisporus derived β-glucan particles were having lowest IC50. Further ROS generation was found to increase in a dose dependent manner. Apoptosis detection was carried out with Propidium iodide and AO/EtBr staining dye which showed significant death in the cells treated with higher concentration of the particles. Study showed that particles derived from both of the sources were having efficient anticancer activity and showing a dose dependent increase in cell death in PC3 cells upon treatment.
Impact of perioperative COVID-19 infection on postoperative complication in cesarean section using Korean National Health insurance data
Authors: Kim Hyo Jin , Ahn EunJin , Kim Gunn Hee , Noh Ji-Hyun , Bang Si Ra
Published: July 2024

The vulnerability during pregnancy has raised concerns about the potential impact of COVID-19 on obstetric anesthesia, an essential aspect of maternal care during cesarean section procedures. To evaluate the influence of COVID-19 infection on obstetric anesthesia during cesarean section, we analyzed the data from Korean National Health Insurance System (NHIS). This retrospective study utilized data from Korean NHIS. We included patients admitted with operation codes specific to cesarean section between January 1, 2020, and December 31, 2021. We classified patients into a COVID (+) group with a diagnosis code (U071) 30 days around surgery and a COVID (−) group without the code in the same period. The primary outcome was 30-day mortality that was defined as death within 30 days of admission due to any causes. Secondary outcomes were pulmonary complications (pneumonia, acute respiratory distress syndrome [ARDS], pulmonary thromboembolism [PTE], or unexpected postoperative mechanical ventilation), ICU admission, cardiac arrest, myocardial infarction [MI], other thromboembolic events, surgical site infection, sepsis, acute renal failure [ARF], and hepatic failure. Among 75,268 patients who underwent cesarean section, 107 had a COVID-19 diagnosis code, while 75,161 did not. After 1:4 propensity score matching (PSM), 535 patients were included in each group. 30-day mortality showed no significant differences between the two groups both before and after PSM. The COVID (+) group demonstrated significantly elevated rates of pneumonia, ARDS, PTE, and surgical site infection both before and after PSM. Hospital length of stay and admission costs were also significantly longer and higher, respectively, in the COVID (+) group before and after PSM. In subgroup analysis, no differences were observed in mortality and postoperative complications based on the anesthesia method after matching. COVID-19 infection is associated with increased rates of postoperative complications, including pneumonia, ARDS, PTE, surgical site infection, longer hospital stays, and increased admission costs, in patients who underwent cesarean section.
The glycine-rich domain of GRP7 plays a crucial role in binding long RNAs and facilitating phase separation
Authors: Lühmann Kim Lara , Seemann Silja , Martinek Nina , Ostendorp Steffen , Kehr Julia
Published: July 2024

Microscale thermophoresis (MST) is a well-established method to quantify protein-RNA interactions. In this study, we employed MST to analyze the RNA binding properties of glycine-rich RNA binding protein 7 (GRP7), which is known to have multiple biological functions related to its ability to bind different types of RNA. However, the exact mechanism of GRP7’s RNA binding is not fully understood. While the RNA-recognition motif of GRP7 is known to be involved in RNA binding, the glycine-rich region (known as arginine-glycine-glycine-domain or RGG-domain) also influences this interaction. To investigate to which extend the RGG-domain of GRP7 is involved in RNA binding, mutation studies on putative RNA interacting or modulating sites were performed. In addition to MST experiments, we examined liquid–liquid phase separation of GRP7 and its mutants, both with and without RNA. Furthermore, we systemically investigated factors that might affect RNA binding selectivity of GRP7 by testing RNAs of different sizes, structures, and modifications. Consequently, our study revealed that GRP7 exhibits a high affinity for a variety of RNAs, indicating a lack of pronounced selectivity. Moreover, we established that the RGG-domain plays a crucial role in binding longer RNAs and promoting phase separation.
Play partner preferences among groups of unfamiliar juvenile male rats
Authors: Ham Jackson R. , Pellis Sergio M.
Published: July 2024

Like many mammals, as juveniles, rats engage in play fighting, which in the laboratory is typically studied in dyads, and consequently, it is the researcher who determines a rat’s play partner. In real-life conditions, a rat would have many partners with whom to play. In a previous study, we found that rats do prefer to play with some individuals more than others, and surprisingly, when given the choice, unfamiliar partners are preferred to familiar ones. In this study, we assessed partner choice when all the available partners are strangers. Eight groups of six unfamiliar juvenile male rats were observed for 10 min play trials. One of the six in each group was selected as the ‘focal’ rat and his play towards, and received by, the others were scored. Social networks revealed that five of the eight groups formed preferences, with preferred partners also engaging in more play with the focal rat. The mechanism by which these preferences were formed remains to be determined, but it seems that there are individual differences, potentially in the amount and style of play, that allow an individual to select the most suitable partner from a group of strangers.
Performance of four different microalgae-based technologies in antibiotics removal under multiple concentrations of antibiotics and strigolactone analogue GR24 administration
Authors: Huang Jing , Wang Zhengfang , Zhao Chunzhi , Yang Huayun , Niu Lei
Published: July 2024

The formation of symbionts by using different combinations of endophytic bacteria, microalgae, and fungi to purify antibiotics-containing wastewater is an effective and promising biomaterial technology. As it enhances the mixed antibiotics removal performance of the bio-system, this technology is currently extensively studied. Using exogenous supplementation of various low concentrations of the phytohormone strigolactone analogue GR24, the removal of various antibiotics from simulated wastewater was examined. The performances of Chlorella vulgaris monoculture, activated sludge–C. vulgaris–Clonostachys rosea, Bacillus licheniformis–C. vulgaris–C. rosea, and endophytic bacteria (S395-2)–C. vulgaris–C. rosea co-culture systems were systematically compared. Their removal capacities for tetracycline, oxytetracycline, and chlortetracycline antibiotics from simulated wastewater were assessed. Chlorella vulgaris–endophytic bacteria–C. rosea co-cultures achieved the best performance under 0.25 mg L−1 antibiotics, which could be further enhanced by GR24 supplementation. This result demonstrates that the combination of endophytic bacteria with microalgae and fungi is superior to activated sludge–B. licheniformis–microalgae–fungi systems. Exogenous supplementation of GR24 is an effective strategy to improve the performance of antibiotics removal from wastewater.
Millimeter wave negative refractive index metamaterial antenna array
Authors: Aziz Rao Shahid , Koziel Slawomir , Pietrenko-Dabrowska Anna
Published: July 2024

In this paper, a novel negative refractive index metamaterial (NIM) is developed and characterized. The proposed metamaterial exhibits negative effective permittivity (εeffe) and negative effective permeability (µeffe) at millimeter wave frequency of 28 GHz. This attractive feature is utilized to enhance the gain of a microstrip patch antenna (MPA). Two thin layers of 5 × 5 subwavelength unit cell array of NIM are placed above a single MPA to enhance the gain of the antenna. Each unit cell has an area of 3.4 × 3.4 mm2. A gain increase of 7.9 dBi has been observed when using the proposed NIM as a superstrate. Furthermore, the NIM array is placed over a 2 × 2 array of MPAs with four ports to demonstrate versatility of the metamaterial. The total size of the 2 × 2 antenna array system with N-MTM is about 61.1 × 34 × 16mm3(5.71λ × 3.18λ × 1.5λ, whereλis the free-space wavelength at 28 GHz). The measurement result indicate that the maximum gain of the antenna array is 13.5dBi. A gain enhancement of 7.55 dB in E-Plane and 7.25 dB in H-Plane at the resonant frequency of 28 GHz is obtained. The proposed antenna structure is suitable for 5G millimeter wave communications, in particular, for possible implementation in future millimeter wave access points and cellular base stations.
Digital biomarkers for non-motor symptoms in Parkinson’s disease: the state of the art
Authors: Janssen Daalen Jules M. , van den Bergh Robin , Prins Eva M. , Moghadam Mahshid Sadat Chenarani , van den Heuvel Rudie , Veen Jeroen , Mathur Soania , Meijerink Hannie , Mirelman Anat , Darweesh Sirwan K. L. , Evers Luc J. W. , Bloem Bastiaan R.
Published: July 2024

Digital biomarkers that remotely monitor symptoms have the potential to revolutionize outcome assessments in future disease-modifying trials in Parkinson’s disease (PD), by allowing objective and recurrent measurement of symptoms and signs collected in the participant’s own living environment. This biomarker field is developing rapidly for assessing the motor features of PD, but the non-motor domain lags behind. Here, we systematically review and assess digital biomarkers under development for measuring non-motor symptoms of PD. We also consider relevant developments outside the PD field. We focus on technological readiness level and evaluate whether the identified digital non-motor biomarkers have potential for measuring disease progression, covering the spectrum from prodromal to advanced disease stages. Furthermore, we provide perspectives for future deployment of these biomarkers in trials. We found that various wearables show high promise for measuring autonomic function, constipation and sleep characteristics, including REM sleep behavior disorder. Biomarkers for neuropsychiatric symptoms are less well-developed, but show increasing accuracy in non-PD populations. Most biomarkers have not been validated for specific use in PD, and their sensitivity to capture disease progression remains untested for prodromal PD where the need for digital progression biomarkers is greatest. External validation in real-world environments and large longitudinal cohorts remains necessary for integrating non-motor biomarkers into research, and ultimately also into daily clinical practice.
More urbanization, more polarization: evidence from two decades of urban expansion in China
Authors: Hu Yi’na , Connor Dylan Shane , Stuhlmacher Michelle , Peng Jian , Turner II B. L.
Published: July 2024

This article examines whether the patterns of urbanization in China over the past twenty years have resulted in a more balanced or increasingly polarized city size distribution. China is an important study area due to the immense scale of its urbanization and the substantial role of its planners in coordinating and striving for more balanced development patterns. This study utilizes measures of the spatial and temporal characteristics of built-up area (BUA) expansion across 23 urban agglomerations in China, focusing on size dynamics through the urban primacy index and Pareto exponent. The BUA within these 23 UAs increased by 280.90% in the study period. Coastal urban agglomerations were larger in size at baseline and maintained relatively high annual growth rates compared to inland agglomerations, indicating rising polarization. Changes within agglomerations were indicative of declining polarization, as evidenced by the reduction in the urban primacy index and the increase of Pareto exponent. Policies implemented to achieve enhanced urbanization coordination in China appear to be partially successful, but the growth of urban regions with existing large size continues to fuel uneven urban development and polarization.