bluuebunny/crossref_metadata_2025 huggingface dataset:
A new protein glosaxin consisting of non-catalytic domains of type piii metalloproteinase from the venom of pit viper Gloydius saxatilis inhibits nicotinic acetylcholine receptor
Authors: Osipov A. V. , Kryukova E. V. , Ojomoko L. O. , Shelukhina I. V. , Ziganshin R. H. , Starkov V. G. , Andreeva T. V. , Tsetlin V. I. , Utkin Yu. N.
Published: August 2024

Previously, we found that the venom of the pit viper Gloydius saxatilis inhibited the muscle-type nicotinic acetylcholine receptor (nAChR). Using liquid chromatography, a protein glosaxin was isolated from the venom that inhibited the binding of the α-bungarotoxin to the nAChR of muscle type from Torpedo californica. The amino acid sequence of the isolated protein was analyzed by high resolution mass spectrometry. Subsequent bioinformatic analysis showed that it is homologous to the amino acid sequences of disintegrin-like proteins, consisting of non-catalytic domains of type PIII metalloproteinases from the venom of pit vipers of genus Gloydius. A study of the biological activity of the isolated protein showed that it inhibits the binding of α-bungarotoxin to Torpedo californica nAChR with IC50 = 51 μM. The protein also inhibited acetylcholine-induced functional responses of the human neuronal nAChR of α3β2 subtype. This is the first evidence of the ability of proteins consisting of non-catalytic domains of snake venom type PIII metalloproteinase to inhibit the nAChR.
Isoforms of the cytoskeletal lim-domain protein zyxin in the early embryogenesis of Xenopus laevis
Authors: Ivanova E. D. , Parshina E. A. , Zaraisky A. G. , Martynova N. Y.
Published: August 2024

Zyxin is a conserved mechanosensitive LIM-domain protein that regulates the assembly of F-actin filaments at cell junctions. At the same time, under mechanical stress, it can move from focal adhesions to stress fibrils and into the nucleus and affect gene expression. In Xenopus laevis embryonic cells, western blots with antibodies against Zyxin’s N-terminal and C-terminal LIM-domain regions revealed two full-length and two short isoforms. The intracellular localization of these isoforms and the number depending on the stage of embryo development were determined. According to our study, full forms with different electrophoretic mobility are localized differently in the cell, and the shortest isoform containing LIM-domains is stable during development, mainly located in the nucleus, and participates in gene expression regulation. This study may be of great value to understanding how the LIM-domain mechanotransducer proteins jointly influence morphogenesis and differentiation in vertebrates at early stages of development.
Derivatives of (5-Oxooxazolidin-4-yl)acetic acids in aspartic acid α-carboxyl protection: a word of caution
Authors: Azev V. N. , Chulin A. N. , Molchanov M. V. , Miroshnikov A. I.
Published: August 2024

Isomeric products could be isolated in a reaction of pentafluorophenyl ester of (S)-2-(3-((benzyloxy)-carbonyl)-5-oxooxazolidin-4-yl)acetic acid with aminoacid esters. One product is an expected dipeptide, the isomeric one is an N-hydroxymethylamino succinimide – a product of methylene bridge cleavavge. Steric bulkness of aminoacid esters favors the formation of a peptide, however the reaction selectivity is quite unpredicatable. Moreover, the precise structure assignement requires high-temperature NMR experiments.
Hybrid proteins containing proteorhodopsin from Exiguobacterium sibiricum
Authors: Petrovskaya L. E. , Kryukova E. A. , Bolshakov V. A. , Lukashev E. P. , Siletsky S. A. , Mamedov M. D. , Sudakov R. V. , Dolgikh D. A. , Kirpichnikov M. P.
Published: August 2024

The genes of hybrid proteins including Exiguobacterium sibiricum proteorhodopsin (ESR) and various N-terminal soluble domains have been constructed. Effective synthesis in Escherichia coli cells was observed only in the case of hybrids with chaperone Caf1M and maltose-binding protein MBP expressed as precursors with their own signal sequences. The study of the isolated MBP-ESR protein in micelles and proteoliposomes demonstrated formation and decay of the main photocycle intermediates at pH 8. The photoelectric response of the hybrid proteins Caf-ESR and MBP-ESR is comparable in amplitude to the wild-type ESR response, indicating their homogeneous orientation in the membrane. The obtained constructions can be used to create bacterial expression systems for various retinal proteins, ensuring their uniform incorporation into proteoliposomes.
Electron microscopy of stable electrophoretic fractions of natural humic acids – the key to the understanding of their structural organization
Authors: Trubetskaya O. E. , Selivanova O. M. , Rogachevsky V. V. , Trubetskoj O. A.
Published: August 2024

Transmission electron microscopy (TEM) with contrast staining by uranyl acetate solution was used to study morphological differences between soil humic acids (HAs) and their A, B and C + D fractions obtained using coupling preparative low-pressure size exclusion chromatography and analytical polyacrylamide gel electrophoresis. The electrophoretic mobility of fractions varied in order C + D B A. The distribution of various morphological elements between fractions showed that large structures such as vesicle-like formations 70–150 nm long and 30–80 nm wide with clear edges were found exclusively in fraction A and occupied ~55% of the TEM image area. On the other hand, long fibrils with a length of 60–100 nm, a width of 4–6 nm and a thickness of 2–3 nm, as well as their bundles with a length of 150 nm and a diameter of 30–70 nm were identified only in the C + D fraction and occupied ~59 % area of TEM images. Smaller morphological elements such as point particles with a diameter of 2–3 nm, ring particles with a diameter of 4–6 nm, worm-shaped short particles with a length of 20–30 nm, and spheroids with a diameter of 10– 30 nm were observed in all samples, but in varying quantities. Significant morphological differences between the fractions can be explained by their composition, previously established by using a few physico-chemical methods. The ratio Car(165–108 ppm)/Calk(108–0 ppm), or aromaticity index, calculated from 13C-NMR, could be one of the indicators of the various morphological structures formation. The obtained TEM results clearly confirm the supramolecular organization of soil HAs.
The relevance of engineering and technical solutions implemented in the branch of IBCh RAS, and modern problems of vivariums and breeding facility of laboratory animals
Authors: Murashev A. N. , Meshcheryakov F. A.
Published: August 2024

The article discusses the relevance of engineering and technical solutions that were implemented in the Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of Russian Academy of Sciences (IBH RAS) in the 1980s when designing a laboratory building with a vivarium and a nursery of laboratory animals free of pathogens (SPF). Such laboratory animals are used when performing preclinical studies according to the international standard of good laboratory practice (GLP).
The ribonucleoprotein complex factor Ybx1 stabilizes the maternal mRNA of the ssx2ip gene encoding the centrosome maturation protein in Xenopus laevis embryogenesis
Authors: Parshina E. A. , Zaraisky A. G. , Martynova N. Y.
Published: August 2024

Our study investigates the mechanisms that regulate early developmental gene expression in Xenopus laevis frog embryos. Our previous study demonstrated that maternal mRNAs of two developmentally significant genes, the nuclear retinoic acid receptor rxrg and the pluripotency factor pou5f3, form complexes with ribonucleoprotein complex Ybx1. Based on the results of the present study, we determined and demonstrated that the stability of the maternal mRNA ssx2ip, which encodes a conserved protein, also called Msd1 or ADIP, which is involved in centrosome maturation, is dependent on Ybx1. This research shows that Ybx1 forms a ribonucleoprotein complex with ssx2ip mRNA, which is mediated by its cold shock domain (CSD). This study confirms our hypothesis of Ybx1 selectively binding to maternal transcripts. It opens up new opportunities to study new mechanisms of gene expression regulation at the earliest stages of development by searching for possible cis-motifs for recognition by trans-regulators such as Ybx1.
Tree-dimensional structure of the fluorescent complex of bacterial lipocalin triple mutant with chromophore – DiB3-F53L/F74L/L129M
Authors: Rossokhin A. V. , Goryacheva E. A. , Artemyev I. V. , Arhipova S. F. , Gilvanov A. R. , Pletneva N. V. , Pletnev V. Z.
Published: August 2024

The three dimensional structure of DiB3-F53L/F74L/L129M – a triple mutant of the fluorescent non-covalent complex of a genetically engineered variant of the bacterial protein lipocalin Blc with the synthetic GFP-like chromophore M739, has been studied by molecular dynamics methods. It was found that the chromophore binding site in the complex is similar to DiB1 and differs from the alternative site in DiB3. The complex is characterized by increased fluorescence brightness compared to those with other genetically engineered variants of lipocalin, which makes it one of the promising markers of biological objects in cell biology.
Reducing Outgoing Quality Level (OQL) in Textile Manufacturing Through Six Sigma Methodology: A Case Study
Authors:   Department of Industrial Engineering and Management, Dawood University of Engineering and Technology, Pakistan, Kaka Shuaib , Maryam Muttahira ,   Department of Industrial Engineering and Management, Dawood University of Engineering and Technology, Pakistan, Wassan Rano Khan ,   Department of Industrial Engineering and Management, Dawood University of Engineering and Technology, Pakistan, Sarwar Umair ,   Department of Industrial Engineering and Management, Dawood University of Engineering and Technology, Pakistan, Akbar Ali ,   Department of Industrial Engineering and Management, Dawood University of Engineering and Technology, Pakistan, Ansari Jansher ,   Department of Industrial Engineering and Management, Dawood University of Engineering and Technology, Pakistan
Published: August 2024

Globally, the textile industry plays a significant role in production but faces many challenges that affect its operation and sustainability. Product quality remains an essential concern despite technological advancements and quality control measures. Raw materials, production methods, and skills differ, resulting in customer dissatisfaction and damage to reputation. A quality management strategy is necessary to overcome these problems and promote quality improvement. This includes the implementation of the Lean Six Sigma approach. This article focuses on improving textile quality by developing the Six Sigma DMAIC method. An analysis of three months' data from a textile company shows that an increase in the Outgoing Quality Level (OQL) from 3% to 6% resulted in significant economic losses, amounting to PKR-240,000 due to rework, residual costs, customer returns, and lost sales time. By utilizing Six Sigma tools, the company reduced OQL from 6% to 4%, thus lowering product losses and increasing operational efficiency. As a result of these findings, the textile industry can improve quality and profitability through a Lean Six Sigma approach.
ANALISIS IMPLEMENTASI TUPOKSI MADRASAH DI MADRASAH TSANAWIYAH PONDOK PESANTREN DARUSSALAM KUNIR
Authors: Apit Muhamad , Suklani Suklani
Published: August 2024

ABSTRAK
Dalam konteks manajemen dan administrasi pendidikan. Memahaimi secara mendalam bagaimana madrasah mengimplementasikan Tupoksi yang telah ditetapkan, dengan mencapai tujuan-tujuan yang diharapkan dapat memberikan gambaran yang komprehensif tentang implementasi Tupoksi di madrasah serta memberikan kontribusi positif terhadap upaya perbaikan dan pengembangan lembaga pendidikan tersebut.
Makalah analisis implementasi Tupoksi madrasah, pemilihan metode penelitian sangat penting untuk mendapatkan data yang relevan dan akurat. Berikut adalah beberapa Teknik pengumpulan data. 1). Wawancara Metode   wawancara   digunakan  untuk  memperoleh   keterangan   terkait   tujuan penelitian   dengan   cara   Tanya   jawab   sambil   bertatap   muka   antara pewawancara dengan responden/ orang yang di wawancarai, denganatau   tanpa menggunakan   pedoman   (guide)   wawancara.
Dalam wawancara  tersebut biasa dilakukan  secara individu maupun  dalambentuk   kelompok,  sehingga di   dapat   data   informatik   yang  orientik (Iryana dan Kawastanti, 2016). 2). Metode Dokumentasi Metode dokumentasi dilakukan sebagai proses pengumpulan   data dalam   penyelidikan   atau   penelitian   yang   berbentuk   dokumen dokumen untuk memperoleh beberapa keterangan atau informasi yang diperoleh,   termasuk   catatan-catatan   penting   pelaksanaan   proseskegiatan belajar terkait dengan proses pendidikan. (Sugiyono 2018:476)
tupoksi madrasah, proses, tugas, produk
FUNGSI DAN PERAN KEPEMIMPINAN KIAI DALAM PENGEMBANGAN PONDOK PESANTREN DARUSSALAM KUNIR
Authors: Aminuddin Aminuddin , Suklani Suklani
Published: August 2024

Pesantren sudah ada sejak zaman Hindu-Buddha.Oleh karena itu, dapat disimpulkan bahwa pesantren memberikan kontribusi yang signifikan untuk meningkatkan kehidupan masyarakat Indonesia. Metode kepemimpinan kyai yang sentralistik dalam manajemen dan pengasuh pesantren. Kepemimpinan individual kyai, otoriter-paternalistik, dan feudal karismatik, yang menerapkan manajemen alami yang serba "tidak formal", diidentifikasi sebagai kekuatan yang sangat kuat dan menentukan ini. Fokus penelitian ini adalah kepemimpinan kiai, peran dan fungsi kiai, dan dampak kepemimpinan ini terhadap pertumbuhan Pondok Pesantren. Pola kepemimpinan kiai dalam membangun lembaga pendidikan saat ini sering menjadi perhatian. Jika diperhatikan, banyak kiai bergantung pada charisma daripada praktik kepemimpinan demokratis dan seringkali otoriter. Tujuan penelitian ini adalah untuk: 1. Menentukan fungsi-fungsi kepemimpinan kiai dalam pengembangan Pondok Pesantren Darussalam Kunir; 2. Menentukan peran-peran kepemimpinan kiai dalam pengembangan Pondok Pesantren Darussalam Kunir; dan 3. Menganalisis bagaimana fungsi dan peran kepemimpinan kiai berhubungan satu sama lain. 1. Memberikan gambaran yang lebih jelas tentang peran dan fungsi kepemimpinan kyai dalam pengembangan pondok pesantren Darussalam Kunir. 2. Membantu kyai dalam meningkatkan kualitas kepemimpinannya. 3. Membantu pemerintah dan masyarakat dalam mendukung pengembangan Pondok Pesantren Darussalam Kunir. Penelitian ini dilakukan melalui pendekatan kualitatif deskriptif dan induktif yang dikombinasikan dengan pendekatan fenomenologi sosial. Data berasal dari sumber non-manusia dan manusia; keduanya dikumpulkan melalui observasi, wawancara, dan studi dokumen.
Hasil penelitian menunjukkan bahwa kiai, dalam hal sarana dan prasarana, manajemen, sumber daya manusia, kurikulum, dan pengabdian terhadap masyarakat, memainkan peran dan fungsi penting dalam perkembangan pondok pesantren Darussalam Kunir.
MEMANFAATKAN KECERDASAN BUATAN UNTUK MENINGKATKAN PROSES EVALUASI PEMBELAJARAN DI SEKOLAH MENENGAH SUATU TINJAUAN TERHADAP POTENSI DAN TANTANGANNYA
Authors: Cahyanto Iman , Sonjaya Nana Supriatna
Published: August 2024

Dalam konteks pendidikan di sekolah menengah, evaluasi pembelajaran memainkan peran penting dalam mengukur pencapaian kompetensi siswa dan efektivitas metode pengajaran. Meskipun telah ada berbagai pendekatan evaluasi yang digunakan, implementasi kecerdasan buatan/Artificial intelligence (AI) menawarkan potensi baru untuk meningkatkan proses evaluasi tersebut. Artikel ini menyajikan tinjauan terhadap potensi dan tantangan penerapan kecerdasan buatan dalam evaluasi pembelajaran di sekolah menengah. Dengan menganalisis kajian literatur yang relevan, metode penelitian, hasil penelitian, dan pemikiran diskursif, artikel ini menggambarkan peran AI dalam meningkatkan efisiensi, obyektivitas, dan kedalaman analisis evaluasi pembelajaran. Penelitian-penelitian sebelumnya menyoroti berbagai pendekatan AI yang telah diterapkan, termasuk penggunaan algoritma pembelajaran mesin untuk menilai kinerja siswa dan menyediakan umpan balik yang personal. Namun, tantangan seperti ketersediaan infrastruktur teknologi, perbedaan dalam pemahaman konsep AI di antara para pendidik, dan kekhawatiran etis perlu diatasi agar implementasi AI dalam evaluasi pembelajaran menjadi berhasil. Dengan memahami potensi dan tantangan ini, artikel ini menyimpulkan dengan merekomendasikan langkah-langkah strategis untuk menerapkan kecerdasan buatan secara efektif dalam konteks evaluasi pembelajaran di sekolah menengah.
Kata kunci : Kecerdasan Buatan, Evaluasi Pembelajaran, Sekolah Menengah
KOMPETENSI KEPRIBADIAN KEPALA SEKOLAH DAN MOTIVASI BERPRESTASI GURU SERTA KONTRIBUSINYA TERHADAP KINERJA SEKOLAH DASAR
Authors: Fatimah Siti , Yudaningsih Nunik , Supriadi Andri
Published: August 2024

Abstrak-Kinerja guru sekolah dasar negeri di gugus Wira Widya Kecamatan Gabuswetan Kabupaten Indramayu dinilai belum maksimal. Pada saat dilakukan observasi awal, hal tersebut diduga disebabkan oleh rendahnya kompetensi kepribadian kepala sekolah dan motivasi berprestasi guru. Oleh karena itu untuk menguji kebenarannya, perlu dilakukan penelitian. Penelitian ini menggunakan metode survey dengan pendekatan kuantitatif, serta teknik korelasional dan regresi. Teknik pengumpulan data melalui angket skala Likert terhadap 76 responden yang merupakan guru sekolah dasar negeri di gugus Wira Widya Kecamatan Gabuswetan Kabupaten Indramayu. Hasil analisis data menunjukkan bahwa (1) Terdapat kontribusi yang signifikan kompetensi kepribadian kepala sekolah terhadap kinerja guru sekolah dasar, (2) Terdapat kontribusi yang signifikan motivasi berprestasi guru terhadap kinerja guru sekolah dasar, (3)  Terdapat kontribusi yang signifikan kompetensi kepribadian kepala sekolah dan motivasi berprestasi guru secara simultan terhadap kinerja guru sekolah dasar. Berdasarkan temuan maka peneliti menyarankan pelatihan dan pengembangan dalam aspek kompetensi kepribadian seperti normatif, keteladanan, dan sikap bagi kepala sekolah, memberikan insentif atau penghargaan kepada guru yang mencapai hasil yang baik, mengadakan diskusi dan kolaborasi antara kepala sekolah dan guru juga dapat membantu meningkatkan komunikasi dan pemahaman bersama, yang pada gilirannya dapat memengaruhi kinerja sekolah dasar secara positif.
 Kata Kunci: Kinerja guru, Kompetensi kepribadian, Motivasi berprestasi guru.
Problems of the Formulation of Asset Forfeiture Resulting from Corruption Crimes: A Comparative and Conceptual Study
Authors: Sry Karina Br. Sinuhaji  , Marie Mahfudz Harahap
Published: August 2024

Corruption in Indonesia has long been a severe transnational issue requiring appropriate handling. Although Law No. 31 of 1999 and Law No. 20 of 2001 on the Eradication of Corruption have provided a legal basis for combating corruption, weaknesses remain in their implementation. This study aims to evaluate the effectiveness of these laws by employing a legislative approach and analyzing the opinions of scholars. The primary focus of this research is on asset confiscation resulting from corruption, as regulated by the Anti-Corruption Law, which serves as a key instrument in combating corruption. The findings indicate that unclear legal norms and weak coordination among law enforcement agencies still hinder the implementation of asset confiscation. The conclusion of this study emphasizes the need for regulatory improvements and enhanced coordination among institutions to maximize the effectiveness of asset confiscation in efforts to eradicate corruption in Indonesia
Child and adolescent self-harm in a pandemic world: Evidence from a decade of data
Authors: Cawthorpe David
Published: August 2024

BACKGROUND: Little is known about the COVID-19 pandemic impact on child and adolescent mental health, specifically self-harm. This paper serves to form a basis for understanding and planning an appropriate response to the present and longstanding child and adolescent mental health needs with global recommendations for integrated community support and disaster preparedness.

METHODS: Anonymous, aggregated data from an established regional child and adolescent addictions and mental health service was employed to examine differences in the rates of self-harm as the primary reason for referral among the health-seeking population represented by quarter by year from 2010-2022 to examine whether self-harm rates have increased since the onset of the COVID-19 pandemic.

RESULTS: Females and self-defined sex had higher rates of self-harm referrals compared to males. Both total and first-time self-harm referrals since the COVID outbreak in 2020 did not exceed the highest quarterly rates before COVID since 2010.

DISCUSSION: Since the COVID-19 pandemic, self-harm rates in one Canadian region remain stable and lower than the highest rates previously observed over the last decade. Given misplaced alarmist news and reports, a coherent, evidence-based, dynamic national response to mental health, social support, and disaster planning is required to fully understand how best to respond to the pandemic in general with a sustainable social support and disaster preparedness policy strategy and specifically the ongoing and pandemic-related mental health needs of the child and adolescent help-seeking population.
Measurement of Temperature using Advanced Computing Device
Authors: N. N. Kumbhar  , G. B. Bhagat  , K. K. Gawade  , Dr. S. K. Tilekar  , Dr. P.V. Mane-Deshmukh
Published: August 2024

The temperature parameter plays a vital role in various fields. The human body also has some amount of temperature in a specific range. In medical treatment, temperature should be maintained in the human body according to the age group. The present research article has tried to monitor the temperature of the baby incubator for better treatment of newborn babies than the old treatment. For such a study, we deployed the PSoC62 microcontroller. The results have been displayed with the help of a UART or TFT display. The displayed data is compared to an analog thermometer and a digital voltmeter. The LM35`s output is 10 mV/°C, as shown in the digital voltmeter. The water is used for the same experiment in the lab. The temperature of water is measured during heating and cooling. The measured temperature is between 81°C to 27°C. All the measured temperatures are shown in Table 1. The result of this study is that the temperature of the analog thermometer and the kit reading are the same.
Efectos de las instrucciones y descripciones con y sin criterio en la adquisición y transferencia de una discriminación condicional de segundo orden
Authors:   Universidad de Guadalajara, Ribes-Iñesta Emilio , Zaragoza Scherman Alejandra ,   Universidad de Guadalajara
Published: August 2024

Se realizaron tres experimentos para evaluar los efectos del uso de instrucciones y descripciones de la ejecución, con y sin criterio de solución, en la facilitación de interacciones sustitutivas de contingencias. Participaron voluntariamente 32 estudiantes universitarios de ambos sexos, que se asignaron aleatoriamente a ocho grupos, seis experimentales y dos de control. La tarea experimental consistió en una igualación de la muestra de segundo orden. En el Experimento 1 se evaluó el efecto de entrenar en un primer bloque con instrucciones especificando el criterio (Grupo 1) o especificando la respuesta correcta (Grupo 2) sobre el desempeño en pruebas de transferencia intramodal, extramodal, extrarelacional y extradimensional, así como en la adquisición de una nueva tarea bajo entrenamiento instrumental no correctivo y el mismo tipo de pruebas de transferencia en un segundo bloque. En el Experimento 2 se evaluó el efecto de que se escogieran descripciones especificando el criterio (Grupo 5) o la respuesta elegida (Grupo 6) durante el entrenamiento en un primer bloque, sobre las mismas pruebas y segundo entrenamiento que en el Experimento 1. En el Experimento 3 se evaluó el efecto conjunto de las instrucciones y descripciones especificando el criterio (Grupo 7) o la respuesta (Grupo 8) durante el entrenamiento, de igual manera que en los primeros dos experimentos. Se utilizaron dos grupos de control, uno bajo entrenamiento instrumental no correctivo (Grupo 3) y otro bajo entrenamiento instrumental correctivo (Grupo 4). Los datos mostraron una mejor ejecución en las pruebas de transferencia y en el segundo bloque de los Grupos 1, 4 y 6. Los resultados se discuten en términos de las interacciones discriminativas y lingüísticas que facilitan los procedimientos empleados.
L’étude de la Théorie de l’Esprit au sein de la Théorie des Cadres Relationnels : Une revue de la littérature sur les réponses relationnelles déictiques
Authors:   Université de Picardie Jules Verne, Amiens, Villatte Matthieu , Monestés Jean-Louis ,   Service Universitaire de Psychiatrie -Neurosciences Fonctionnelles & Pathologies – CNRS UMR 8160 - CentreHospitalier Ph. Pinel, McHugh Louise ,   University of Wales, Swansea, Freixa i Baqué Esteve ,   Université de Picardie Jules Verne, Amiens
Published: August 2024

La Théorie de l’Esprit fait référence à la capacité d’attribuer un état mental à soi-même ou à autrui. Alors que ce phénomène fait l’objet d’une littérature abondante en psychologie cognitive depuis plus de vingt ans, l’Analyse du Comportement ne s’est penchée sur cette question que très récemment, au sein de la Théorie des Cadres Relationnels (TCR). Cette approche consiste à analyser le langage et la cognition en termes de réponses relationnelles dérivées arbitrairement applicables afin, notamment, de rendre compte de leurs propriétés symboliques. Le modèle proposé par la TCR repose sur le principe que l’attribution d’états mentaux est sous-tendue par les habiletés de prise de perspective qui correspondent à une clase particulière de réponses relationnelles, les réponses relationnelles déictiques. Le présent article propose une revue de la littérature récente sur cette question, ainsi qu’une confrontation avec les modèles émanant de la psychologie cognitive. Les études développementales, les évaluations de déficits dans des populations connues pour présenter des difficultés en Théorie de l’Esprit (autisme, schizophrénie) et les premières applications en termes de remédiation sont exposées. Les premiers résultats sont prometteurs et montrent la pertinence et l’utilité d’une analyse fonctionnelle de ce concept emblématique de la psychologie cognitive.
Identification of cardiac premature senescence markers through GEO database interaction approach
Authors: Susantiningsih Tiwuk , Prijanti Ani Retno , Hardiany Novi Silvia , Fadilah
Published: August 2024

Background: Cardiomyocytes are cardiac muscle cells where premature senescence can occur. The availability of in silico research regarding premature senescence in cardiomyocytes is still limited.
Purpose: This in silico research aims to identify marker of cardiac premature senescence through the GEO database interaction approach.
Methods: This research used the GEO database with the GeneCards website approach using four keywords: premature senescence AND cardiovascular AND cardiomyocytes AND p53 followed by Cytoscape 3.9.1 analysis and StringDB analysis.
Results: From 1,046 proteins obtained, analyzed by using Cytoscape Tools series 3.9.1 resulted to 100 proteins having the highest score and continued with StringDB analysis with 16 proteins.
Conclusion: From the GeneCards, Cytoscape, and StringDB data, analysis of protein interactions in cardiac premature senescence showed that the protein-protein interactions with the highest scores were TP53, CDK2, and PTEN.
ASSESSMENT OF TRANSIENT VIBRATION EFFECTS OF A ROCKING STRUCTURE ON TWO-PARAMETER UPLIFTING FOUNDATION MODEL
Authors: Ugwu Juliet N. , Onyia Micheal E.
Published: August 2024

This study is on assessment of transient vibration of a rocking structure on two-parameter uplifting foundation model. This foundation model, Filonenko-Borodich (F-B) considers effect of continuity and interactions of the structure with the surrounding foundation surface. The foundation model aids uplift because of its elastic and flexible nature. During ground movement, some forces are generated which cause foundation to vibrate, rock and sometimes uplift due to increasing upward forces and then leading to reduction in the spring stiffness and increase in soil flexibility. This sometimes causes upward trend in the structural response which might affect structural integrity and stability. The use of damping and its effects on the structure response considering uplift are evaluated for a structure on two-parameter foundation. Equations describing the motion were developed by summing forces acting on the structure and foundation, considering equilibrium of moments for the conditions of before uplift and during uplift of the system in accordance using Newton’s second law of motion, D’Alembert’s principles. The resulting equations were solved by applying them in Duhamel Integral form and Simpson’s method used for the numerical solution for the structure responses. The result showed an upward trend in the structural responses during uplifting hence uplift of building foundation is not always beneficial.
المستويات التقديرية في تصنيف تلامذة الابتدائية حسب القدرات العقلية والقابليات البيوحركية
Authors: Lec. Hassanein Abdul Razzaq Jawad  , Prof. Dr. Muhammad Jassim Al-Yasiri
Published: August 2024

تمثلت أهمية البحث في معرفة الواقع الذي علية التلامذة بأعمار (10-12) سنه في القدرات العقلية والقابليات البيو حركية، وتصنيف التلامذة الى مجاميع غير متجانسة بحسب القدرات العقلية والقابليات البيو حركية. اذ اشتملت الاجراءات على تصنيف التلامذة حسب قدراتهم العقلية باستخدام المصفوفات المتباينة الملونة للذكاء لــ(جون رافن), واستخدام اختبارات القابليات البيو حركية, لغرض تصنيفهم الى اربع مجاميع والتي هي المجموعة الاولى (عالية القدرات – عالية القابليات), المجموعة الثانية (واطئة القدرات – عالية القابليات), المجموعة الثالثة ( عالية القدرات – واطئة القابليات), المجموعة الرابعة (واطئة القدرات – واطئة القابليات), اما عينة البحث جاءت باختيار بالطريقة الطبقية العشوائية من مجتمع البحث الأصلي بأسلوب توزيع العينات كل حسب الفئة العمرية والمرحلة الدراسية، وبعدد (267) مختبر من أصل (351) تلميذ، وتم استبعاد الاخرين نتيجة عدم الحضور ولأسباب العمر ومشاكل صحية أخرى، وبنسبة مئوية (70%) للمجتمع المبحوث وكان عدد الذكور للمراحل (الرابع- الخامس- السادس) 197 وعدد الاناث للمراحل (الرابع- الخامس- السادس) 154. وجاءت أبرز نتائج الدراسة: ان المديات المعيارية والمستويات والاختلافات تم الحصول عليها من خلال التوزيع الاعتدالي، هناك فروق فردية بين المستويات التلامذة وحسب مراحلهم وفئاتهم العمرية، الدرجات والمستويات التي تم ايجادها من قبل الباحثان تمثل المستوى الحقيقي لا مكانيات عينة البحث، تباين افراد العينة من كلا الجنسين في حصولهم على مستويات مختلفة وحسب درجاتهم المعيارية.
A Data-Driven Approach in Establishing Ideal Quiz Question Timings
Authors: Zamfiroiu Alin , Vasile Denisa , Ciutacu Claudia , Floroiu Iustin
Published: August 2024

In the realm of modern education, the integration of technology has transformed traditional assessment methods. This article explores the implementation of a data-driven approach to optimize quiz question timings, utilizing both a mobile and web application for student evaluation through multiple-choice questions. The study aims to enhance the efficiency of assessments by analyzing student responses and tailoring question durations based on data-driven insights. The research leverages two distinct platforms: a mobile application and a web-based system, both designed to facilitate seamless student engagement. Through these applications, students can access quizzes featuring multiple-choice questions, providing a comprehensive understanding of their knowledge and proficiency in various subjects. The focus of the investigation lies in harnessing the power of data analytics to refine the timing of each question, thereby optimizing the overall assessment experience. The findings from this study contribute valuable insights into the dynamics of student engagement and performance during assessments. By identifying optimal question timings, educators can create a more adaptive and personalized learning environment. This approach not only benefits students by aligning assessments with their cognitive processes but also aids educators in refining their teaching strategies based on real-time, data-driven feedback. Furthermore, the article delves into the technological aspects of the mobile and web applications, highlighting their user-friendly interfaces, secure assessment environments, and compatibility with various devices. The implementation of these applications reflects a commitment to modernizing educational practices while ensuring accessibility and inclusivity.
Changing Anxiety and Generativity Awareness: Based on a Survey in the COVID-19 Pandemic
Authors: Isada Yuriko
Published: August 2024

This paper examines how generativity awareness and anxiety levels have changed among Japanese people aged 35 and older due to the societal impacts of COVID-19 and the rise of AI in workplaces. The research involved 1,000 participants and utilized surveys conducted during and after the pandemic. It identified four key aspects of generativity awareness: nurturing the next generation, personal growth, creativity, and a shift away from self-centred attitudes. The findings show that while self-anxiety decreases with age, anxiety about the next generation increases. There is also a significant relationship between generativity awareness and self-anxiety. The study highlights an increase in anxiety, especially among women, due to the pandemic.
Psychological Well-Being, Curiosity and Perfectionism among Filipino Young Professionals with Quarter Life Crisis: Basis for Existential Positive Psychology Wellness Plan
Authors: Dalan Kris Hanley , Lopez Elna
Published: August 2024

Quarter Life Crisis is a difficult encounter in the life transition from adolescence to early adulthood. This crisis revolves around social comparison, work satisfaction, intimate relationships, career consolidation, preoccupations and fears, struggle from transition, social isolation, and frustration and disappointments. This study examined the psychological well-being, curiosity, and perfectionism among young professionals with a quarter-life crisis. There were 318 participants in this study from two different cities in one province of Region IV-A in the Philippines. The study used a sequential explanatory mixed method design that combines qualitative and quantitative research. Based on the result, the research found that young professionals with quarter-life crises have low psychological well-being, low curiosity, and high perfectionism. The themes, low disposition, poor mindset, and weak personality were extracted from the texts to fully support the respective variables. Furthermore, the research proposed a psychological intervention plan for young professionals experiencing the crisis: the existential positive psychology wellness plan.
UNVEILING THERAPEUTIC TARGETS THROUGH PATHWAY ANALYSIS AND IDENTIFICATION OF DIFFERENTIALLY EXPRESSED GENES IN ULCERATIVE COLITIS
Authors: Abdelrazig Omnia Amir , Fadilah Fadilah , Erlina Linda , Hegar Badriul
Published: August 2024

Objective: This study utilizes integrated bioinformatics to investigate Differentially Expressed Genes (DEGs) and pathways related to ulcerative colitis (UC).
Material and Method: Differentially Expressed Genes were identified from UC patients' colonic mucosal samples and controls using GSE13367 and GSE134025 datasets. Differentially Expressed Genes selection utilized GEO2R and Venn diagrams, followed by functional annotation, pathway analysis, PPI determination via the STRING database, and GO/KEGG enrichment analysis using Metascape.
Result and Discussion: Analysis unveiled 197 DEGs, with 76 up-regulated and 121 down-regulated genes. Up-regulated genes were enriched in humoral immune response, peptidoglycan binding, and NADPH oxidase complex, while down-regulated genes were linked to inorganic anion transport, transmitter-gated ion channel activity, and integral plasma membrane components. In the PPI network, up-regulated DEGs formed a dense network (75 nodes, 190 edges), indicating significant interactions, whereas down-regulated DEGs formed a less dense network (114 nodes, 63 edges). Five hub genes (CXCR4, CXCL13, CXCL1, MMP3) were identified among the 197 DEGs. These findings provide new insights into UC's causes and offer promise for more effective therapeutic approaches.
Fiziksel Şiddet Kaygı Ölçeğinin Geliştirilmesi
Authors: Memduhoğlu Hasan Basri , Demir Mehmet Erkan
Published: August 2024

Bu araştırmanın amacı öğretmelerin fiziksel şiddete ilişkin kaygı düzeylerini belirlemeye yönelik bir ölçek geliştirmektir. Araştırmanın çalışma grubu Siirt ilindeki çeşitli liselerde görev yapan 657 yönetici ve öğretmendir. Ölçeğin yapı geçerliliği için açımlayıcı faktör analizi AFA) tekniği olan temel bileşenler analizi, ortaya çıkan yapıyı test etmek için de Lisrel programı ile doğrulayıcı faktör analizi (DFA) yapılmış ve açımlayıcı faktör analizi sonucu belirlenen faktörler doğrulanmıştır. Maddelerin ayırt ediciliği için madde toplam korelâsyonlarına bakılmış, ölçeğin güvenirlik hesaplamalarında Cronbach’s Alpha değeri dikkate alınmıştır. Araştırmada Fiziksel Şiddet Kaygı Ölçeği’nin (FŞKÖ); olumsuz sonuç kaygısı, maruz kalma kaygısı ve destek algısı olmak üzere üç faktörden oluştuğu ve ölçeğin öğretmenlerin fiziksel şiddete ilişkin kaygılarını ölçebilecek geçerli ve güvenilir bir ölçme aracı olduğu sonucuna ulaşılmıştır.
Sessiz Kitapların Okul Öncesi Dönem Çocuklarının Dil Gelişimlerine Etkisinin İncelenmesi
Authors: Taşkın Zeynep , Durmuşoğlu Mine Canan
Published: August 2024

Bu araştırmada sessiz kitapların okul öncesi dönem çocuklarının dil gelişimine etkisini ortaya koymak amaçlanmaktadır. Araştırma, yarı deneysel desen türlerinden ön-test, son-test, kalıcılık testi eşleştirilmiş kontrol gruplu desen uygulanarak yürütülmüştür. Araştırmanın çalışma grubunu, 2023-2024 eğitim-öğretim yılında Van ili İpekyolu ilçesinde iki ayrı resmi bağımsız anaokuluna devam eden 58 çocuk oluşturmaktadır. Seçkisiz bir şekilde belirlenen anaokullarından birindeki dört sınıf deney, diğer anaokulundaki dört sınıf ise kontrol grubunu oluşturmuştur. Çalışmada veri toplama aracı olarak Kaçar Kütükçü (2021) tarafından geçerlilik ve güvenirlik çalışması yapılmış olan LITMUS Türkçe Cümle Tekrarı Testi kullanılmıştır. Verilerin analizinde betimleyici istatistiklerin yanında fark analizleri yapılmıştır. İkili grupların karşılaştırılmasında bağımsız örneklemler t testi, bağımlı örneklemler t testi ve çoklu grupların karşılaştırılmasında ise bağımlı örneklemler tek yönlü varyans analizi ANOVA testleri kullanılmıştır. Elde edilen bulgulara göre, eğitim ortamının sessiz kitaplar kullanılarak desteklendiği deney grubunun LİTMUS Testi son test sonuçları, herhangi bir müdahalenin bulunmadığı kontrol grubuna göre deney grubunun lehine anlamlı derecede farklılık göstermektedir. Benzer şekilde deney grubu LİTMUS Testi ön test sonuçları ile son test sonuçları arasında da son test lehine anlamlı bir farklılık bulunmaktadır. Bulgular, sessiz kitapların eğitim ortamında kullanımının çocukların dil gelişimine katkı sağladığını ortaya koymaktadır.
Artificial intelligence in the diagnostics and treatment of inflammatory bowel diseases (review)
Authors: Fil’ T. S. , Bakulin I. G.
Published: August 2024

Currently scientists from different countries are exploring the possibilities of using machine learning methods to improve the accuracy of endoscopic and radiation diagnostics in patients with inflammatory bowel diseases (IBD) both to reduce the time spent by doctors on describing the results and to reduce the time needed to verify the diagnosis. Predicting the course of IBD based on artificial intelligence (AI) with the creation of predictive scenarios (models) is another promising area in gastroenterology. This review analyzes the main directions of scientific projects on the introduction of AI and machine learning methods in the diagnosis and prediction of the course of IBD. The article pays special attention to the problems faced by specialists in the application of AI methods, ways to solve them, as well as the prospects for using AI in patients with IBD. The possibilities of using AI for colorectal cancer screening and analysis of medical records are presented.
Effectiveness of 0.3 % nifedipine gel combined with 2.0 % lidocaine and BTA 80 U in treatment of chronic anal fissure. Pilot randomized study. Preliminary results
Authors: Zharkov E. E. , Goloktionov N. A. , Sagidova K. I. , Lebedeva E. Yu. , Kostarev I. V.
Published: August 2024

AIM: to improve the results of chronic anal fissure treatment.

   PATIENTS AND METHODS: the study included 22 patients randomized by random numbers generation method into 2 groups. Eleven patients were included in main group and were treated with 0.3 % nifedipine + 2.0 % lidocaine gel, 11 patients of the control group received injections of botulinum toxin A into the internal anal sphincter at a dose of 80 U (BTA 80).

   RESULTS: by day 30, there was a decrease of maximal resting pressure in anal canal (MRPAC) in both the main and control groups [p = 0.015 and p = 0.004, respectively] and the average resting pressure in anal canal (ARPAC) [p = 0.01 and p = 0.02, respectively]. There was no difference between the groups in pain severity both after stool and during the day (p = 0.5 and p = 0.6, respectively). On day 60, the defect was epithelized in 6/11 (54.6 %) patients of the study group and in 9/11 (81.8 %) patients of the BTA 80 group [p = 0.36], respectively. The reason of treatment failure in 4/11 (36.4 %) patients of the main group and 2/11 (18.2 %) patients of the control group was a preserved internal sphincter spasm. It was found that these patients used a lower amount of the drug product — 2.2 (1.8; 2.5) mg/day compared to 2.4 (1.9; 2.7) mg/day in other patients. On the day 30 after surgical treatment, complaints about gas incontinence were registered in 1/11 (9.1 %) patients of the main group and 1/11 (9.1 %) patients of the control group [p = 1]. Such a complication as external hemorrhoid thrombosis occurred only in 1/11 (9.1 %) patients in the BTA 80 group [p = 0.87].

   CONCLUSION: the study results show that gel containing 0.3 % nifedipine and 2 % lidocaine produces an effect on the tone of the internal sphincter comparable to BTA. However, the lack of accurate dosage of the drug product and/or low patient compliance reduce the effectiveness of treatment and make the use of BTA preferable for medical relaxation of the internal sphincter.
Staged surgical treatment of wounds of the extra-abdominal rectum
Authors: Pesikin I. N. , Perekhodov S. N. , Onnitsev I. E. , Artemkin E. N. , Sokolov A. A. , Kharitonova L. S. , Mytnikov A. D. , Kozlov N. S. , Kelzin A. I. , Kuzminov A. D.
Published: August 2024

AIM: to improve the algorithm and results of surgical treatment of combat wounds of the rectum.

   PATIENTS AND METHODS: thirty-one patients with combat injuries of the rectum were included in the cohort.

   RESULTS: twenty-seven (87.1 %) patients were discharged in satisfactory condition, 4 (12,9 %) patients died. Sepsis developed in all four patients: in two of them sepsis was on the background of necrotising fasciitis and one had peritonitis. Endoscopic control revealed 10 wounds of the extraperitoneal part of the rectum undiagnosed at the 1st and 2nd stages of medical care. Surgical care included all four main components: diverting stoma, distal washout (mechanical cleansing of the rectum to clear water), suturing of the lesion through perineal access, drainage or tamponisation of the presacral space was done in 20 (64.5 %) patients. Endoluminal VAC therapy was used in 12 patients. In 11 of them, VAC treatment was successful with complete healing of rectal wall wounds.

   CONCLUSION: the four-component treatment approach is the optimal option for wounds of the extraperitoneal part of the rectum. Diverting stoma and distal bowel washout prevents severe infection. Endoluminal VAC therapy, in some cases, can be used as an alternative to presacral drainage and suturing of the rectal wall lesion.
Intracorporal isoperistaltic linear colorectal “side-to-side” (overlap) anastomosis: invention concept and primary results
Authors: Sazhin A. V. , Ermakov I. V. , Ivakhov G. B. , Lebedev I. S. , Dalgatov K. D. , Poltoratsky M. V. , Shikhin I. S. , Timoshenko N. A.
Published: August 2024

AIM: to present the primary experience of performing intracorporeal linear colorectal anastomosis using the “overlap” technique in laparoscopic anterior rectal resection.

   MATERIALS AND METHODS: the study presents the outcomes of intracorporeal linear “overlap” anastomosis in patients who underwent laparoscopic anterior rectal resection between October 2023 and February 2024. The results were evaluated based on various parameters including operation time, the postoperative morbidity rate (by Clavien-Dindo scale), conversion rates, hospital stay, quality of life by SF-36 questionnaire 30 days after surgery.

   RESULTS: the linear “overlap” anastomosis was done in 10 patients (5 females), aged 62.0 ± 9.03 years and BMI of 27.5 ± 7.2 kg/m2. No intraoperative complications occurred. One patient developed a seroma at the incision site (Clavien–Dindo I). The first bowel movement was at 1 (0–3) days after surgery. The post-op hospital stay was 5.7 ± 1.9 days.

   CONCLUSION: primary results demonstrate the simplicity and safety of intracorporeal linear “overlap” anastomosis technique. The further advanced study is justified.
Open management of rectal wound after transanal endoscopic microsurgery: randomized prospective study
Authors: Sinitsyn R. K. , Alekseev M. V. , Chernyshov S. V. , Khomyakov E. A. , Rybakov E. G.
Published: August 2024

INTRODUCTION: the wound following Transanal Endoscopic Microsurgery (TEM) is traditionally closed by suturing. However, some studies suggest that leaving the wound open after TEM might be a viable alternative.

   STUDY OBJECTIVE: to compare two methods of managing rectal wounds in patients after TEM.

   PATIENTS AND METHODS: patients were divided into 2 groups: the main group (open wound management) and the control group (suture wound management). The primary endpoint of the study was the morbidity rate. The secondary endpoints included the need and duration of antibacterial therapy, post-op hospital stay, the severity of the inflammatory response, the wound healing time and the quality of life.

   RESULTS: from November 2021 to November 2023, 177 patients were included in the randomized study: 68 in the open wound management group and 109 in the suture wound group. The analysis included 50 patients in eachgroup. It was found that the operation time in the main group was significantly shorter (30 (20; 40) minutes versus 55 (40; 60), p < 0.0001). The infectious complications rate was lower in the open wound management group: 11/50 (22 %) versus 16/50 (32 %), p = 0.26; the postoperative bleeding rate was higher: 4/50 (8 %) versus 2/50 (4 %), p = 0.7. Post-op hospital stay did not differ: 6 (4;7) days versus 6 (5; 7), p = 0.22. The duration of antibiotic therapy for infectious complications was longer in the open wound management group: 6 (5;6) days versus 5 (5;6.5), p = 0.02. In the case of infectious complication the inflammatory response was higher in the group of the open wound management: white blood cell counts (15.9 (14.3; 19.5) × 109/L versus 13.1 (12; 15.6) × 109/L, p = 0.01) and temperature (38.6 ± 0.7 °C versus 38 ± 0.6 °C, p = 0.02). Wounds in both groups healed by the 30th day, and the quality of life did not differ between groups.

   CONCLUSION: the results of the randomized study demonstrated the efficacy and safety of open rectal wound management after TEM.