bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of LncRNA MSTRG.20890.1 in Hair Follicle Development of Cashmere Goats
Authors: Wang Min , Ma Rong , Ma Qing , Ma Bingjie , Shang Fangzheng , Lv Qi , Wang Zhiying , Wang Ruijun , Su Rui , Zhao Yanhong , Zhang Yanjun
Published: October 2024

Background: The cashmere goat is a biological resource that mainly produces cashmere. Cashmere has a soft hand feel and good luster, with high economic value. The quality and yield of cashmere are determined by the process of hair follicle development during the embryonic period. Methods: In this study, the skin of the Inner Mongolia cashmere goat at different embryonic stages (45, 55, 65, and 75d) was collected, and the differentially expressed lncRNA MSTRG.20890.1 at 75d was obtained by screening. Dual luciferase reporter gene system, qRT-PCR, and EDU experiments were used to verify further the regulatory role and molecular mechanism of the lncRNA in dermal fibroblasts. Results: Based on the transcriptome database of Inner Mongolia cashmere goat skin at different embryonic stages, which was previously constructed by our group, according to the characteristics of hair follicle development in the embryonic stage, we screened out the lncRNA MSTRG.20890.1 that was down-expressed on the 75-SHFINI day of the embryonic stage. We found that lncRNA MSTRG.20890.1 was mainly located in the cytoplasm of cells, and it could inhibit the proliferation and directional migration of dermal fibroblasts through the chi-miR-24-3p/ADAMTS3 signaling axis, thereby inhibiting the formation of dermal papilla structure at embryonic stage. Conclusions: This study revealed that lncRNA MSTRG.20890.1 regulated secondary hair follicle morphogenesis and development in cashmere goats through the chi-miR-24-3p/ADAMTS3 signaling axis.
Alternative Splicing: A Potential Therapeutic Target in Hematological Malignancies
Authors: Temaj Gazmend , Chichiarelli Silvia , Saha Sarmistha , Telkoparan-Akillilar Pelin , Nuhii Nexhibe , Hadziselimovic Rifat , Saso Luciano
Published: October 2024

Leukemia represents the most prevalent malignancy in children, constituting 30% of childhood cancer cases, with acute lymphoblastic leukemia (ALL) being particularly heterogeneous. This paper explores the role of alternative splicing in leukemia, highlighting its significance in cancer development and progression. Aberrant splicing is often driven by mutations in splicing-factor genes, which can lead to the production of variant proteins that contribute to oncogenesis. The spliceosome, a complex of small nuclear RNAs and proteins, facilitates RNA splicing, a process critical for generating diverse mRNA and protein products from single genes. Mutations in splicing factors, such as U2AF1, SF3B1, SRSF2, ZRSR2, and HNRNPH1, are frequently observed across various hematological malignancies and are associated with poor prognosis and treatment resistance. This research underscores the necessity of understanding the mechanisms of RNA splicing dysregulation in order to develop targeted therapies to correct these aberrant processes, thereby improving outcomes for patients with leukemia and related disorders.
Bullying in Nursing Students: A Cross-Sectional Study
Authors: Fernández-Gutiérrez Lidia , Mosteiro-Díaz Maria-Pilar , Borges Elisabete , Franco-Correia Sara
Published: October 2024

The presence of violence between coworkers in nursing settings is a common fact that has been documented over time by using different terms to define the violence suffered by nurses and nursing students, although in recent years it has been agreed that the term “bullying” covers them all. This study aims to determine the prevalence and characteristics of bullying in nursing students, and to describe the association between the prevalence of bullying and socio-demographic attributes. A descriptive, observational, quantitative, and cross-sectional study was carried out of nursing students between 2017 and 2020. Socio-demographic variables were collected. Bullying was assessed through the NAQ-R questionnaire. Data were analyzed using the R statistical program. A total of 411 nursing students were included. A prevalence of perceived bullying of 92% was detected. The main negative attitudes identified were mandated tasks that are below the level of competence (66.3%), changes in tasks for other more trivial or unpleasant ones (60%), having opinions that are ignored (52%), and being excessively supervised (49%). The prevalence of bullying in nursing students at the University of Oviedo exceeds 90%, which suggests that interventions to solve the problem of bullying are urgent at the institutional, political, and organizational levels.
Improved Early-Stage Maize Row Detection Using Unmanned Aerial Vehicle Imagery
Authors: Xue Lulu , Xing Minfeng , Lyu Haitao
Published: October 2024

Monitoring row centerlines during early growth stages is essential for effective production management. However, detection becomes more challenging due to weed interference and crop row intersection in images. This study proposed an enhanced Region of Interest (ROI)-based approach for detecting early-stage maize rows. It integrated a modified green vegetation index with a dual-threshold algorithm for background segmentation. The median filtering algorithm was also selected to effectively remove most noise points. Next, an improved ROI-based feature point extraction method was used to eliminate residual noises and extract feature points. Finally, the least square method was employed to fit the row centerlines. The detection accuracy of the proposed method was evaluated using the unmanned aerial vehicle (UAV) image data set containing both regular and intersecting crop rows. The average detection accuracy of the proposed approach was between 0.456° and 0.789° (the angle between the fitted centerline and the expert line), depending on whether crop rows were regular/intersecting. Compared to the Hough Transform (HT) algorithm, the results demonstrated that the proposed method achieved higher accuracy and robustness in detecting regular and intersecting crop rows. The proposed method in this study is helpful for refined agricultural management such as fertilization and irrigation. Additionally, it can detect the missing-seedling regions and replenish seedings in time to increase crop yields.
Tail Tales: What We Have Learned About Regeneration from Xenopus Laevis Tadpoles
Authors: Lara Jessica , Mastela Camilla , Abd Magda , Pitstick Lenore , Ventrella Rosa
Published: October 2024

This review explores the regenerative capacity of Xenopus laevis, focusing on tail regeneration, as a model to uncover cellular, molecular, and developmental mechanisms underlying tissue repair. X. laevis tadpoles provide unique insights into regenerative biology due to their regeneration-competent and -incompetent stages and ability to regrow complex structures in the tail, including the spinal cord, muscle, and skin, after amputation. The review delves into the roles of key signaling pathways, such as those involving reactive oxygen species (ROS) and signaling molecules like BMPs and FGFs, in orchestrating cellular responses during regeneration. It also examines how mechanotransduction, epigenetic regulation, and metabolic shifts influence tissue restoration. Comparisons of regenerative capacity with other species shed light on the evolutionary loss of regenerative abilities and underscore X. laevis as an invaluable model for understanding the constraints of tissue repair in higher organisms. This comprehensive review synthesizes recent findings, suggesting future directions for exploring regeneration mechanisms, with potential implications for advancing regenerative medicine.
The Complement System as a Part of Immunometabolic Post-Exercise Response in Adipose and Muscle Tissue
Authors: Wojciuk Bartosz , Frulenko Ignacy , Brodkiewicz Andrzej , Kita Dagmara , Baluta Monica , Jędrzejczyk Filip , Budkowska Marta , Turkiewicz Karolina , Proia Patrizia , Ciechanowicz Andrzej , Kostrzewa-Nowak Dorota , Nowak Robert
Published: October 2024

The precise molecular processes underlying the complement’s activation, which follows exposure to physical stress still remain to be fully elucidated. However, some possible mechanisms could play a role in initiating changes in the complement’s activity, which are observed post-exposure to physical stress stimuli. These are mainly based on metabolic shifts that occur in the microenvironment of muscle tissue while performing its function with increased intensity, as well as the adipose tissue’s role in sterile inflammation and adipokine secretion. This review aims to discuss the current opinions on the possible link between the complement activation and diet, age, sex, and health disorders with a particular emphasis on endocrinopathies and, furthermore, the type of physical activity and overall physical fitness. It has been indicated that regular physical activity incorporated into therapeutic strategies potentially improves the management of particular diseases, such as, e.g., autoimmune conditions. Moreover, it represents a favorable influence on immunoaging processes. A better understanding of the complement system’s interaction with physical activity will support established clinical therapies targeting complement components.
Fahr’s Syndrome with Pseudohypoparathyroidism: Oral Features and Genetic Insights
Authors: Wang Xiangpu , Xu Taoyun , Zhu Yulong , Duan Xiaohong
Published: October 2024

Fahr’s syndrome is a rare neurodegenerative disorder with limited research on its oral manifestations. This study investigates the dental features and genetic background of Fahr’s syndrome through a pedigree analysis and a retrospective literature study. A clinical examination and whole-exome sequencing (WES) were conducted on a female patient with Fahr’s syndrome and pseudohypoparathyroidism, along with her family members. The patient presented with super-numerary teeth, tooth agenesis, enamel hypoplasia, and abnormal tooth eruption. The WES did not reveal any known pathogenic mutations related to pseudohypoparathyroidism or Fahr’s disease. However, genetic variations in KIF1A, FZD8, and PDGFA may underlie these dental abnormalities. Additionally, a retrospective analysis of 22 reported cases from PubMed and the Human Gene Mutation Database (1 January 1965–30 June 2024) was conducted with keywords such as “Fahr’s disease”, “Fahr’s syndrome”, “dental”, and “hypoparathyroidism”. The analysis showed that patients with Fahr’s syndrome, pseudohypoparathyroidism, and idiopathic hypoparathyroidism exhibited similar oral abnormalities, including tooth agenesis, root dysplasia, dental malformations, and abnormal tooth eruption. Variations in the incidence of tooth agenesis and dental malformation among these groups may be linked to differences in parathyroid hormone metabolism. These findings suggest oral abnormalities are the key local features of Fahr’s syndrome and related parathyroid disorders.
New Insights into the Fanconi Anemia Pathogenesis: A Crosstalk Between Inflammation and Oxidative Stress
Authors: Repczynska Anna , Ciastek Barbara , Haus Olga
Published: October 2024

Fanconi anemia (FA) represents a rare hereditary disease; it develops due to germline pathogenic variants in any of the 22 currently discovered FANC genes, which interact with the Fanconi anemia/breast cancer-associated (FANC/BRCA) pathway to maintain genome integrity. FA is characterized by a triad of clinical traits, including congenital anomalies, bone marrow failure (BMF) and multiple cancer susceptibility. Due to the complex genetic background and a broad spectrum of FA clinical symptoms, the diagnostic process is complex and requires the use of classical cytogenetic, molecular cytogenetics and strictly molecular methods. Recent findings indicate the interplay of inflammation, oxidative stress, disrupted mitochondrial metabolism, and impaired intracellular signaling in the FA pathogenesis. Additionally, a shift in the balance towards overproduction of proinflammatory cytokines and prooxidant components in FA is associated with advanced myelosuppression and ultimately BMF. Although the mechanism of BMF is very complex and needs further clarification, it appears that mutual interaction between proinflammatory cytokines and redox imbalance causes pancytopenia. In this review, we summarize the available literature regarding the clinical phenotype, genetic background, and diagnostic procedures of FA. We also highlight the current understanding of disrupted autophagy process, proinflammatory state, impaired signaling pathways and oxidative genotoxic stress in FA pathogenesis.
Co-Stimulation with TWEAK and TGF-β1 Induces Steroid-Insensitive TSLP and CCL5 Production in BEAS-2B Human Bronchial Epithelial Cells
Authors: Abe Sumiko , Harada Norihiro , Sandhu Yuuki , Sasano Hitoshi , Tanabe Yuki , Ueda Shoko , Nishimaki Takayasu , Sato Yoshihiko , Takeshige Tomohito , Harada Sonoko , Akiba Hisaya , Takahashi Kazuhisa
Published: October 2024

Steroid-resistant asthma is a common cause of refractory asthma. Type 2 inflammation is the main inflammatory response in asthma, and the mechanism underlying the steroid-resistance of type 2 inflammation has not been completely elucidated. Tumor-necrosis-factor-like apoptosis-inducing factor (TWEAK) and transforming growth factor (TGF)-β1 are involved in epithelial–mesenchymal transition (EMT) and the production of thymic stromal lymphopoietin (TSLP) and C-C motif chemokine ligand 5 (CCL5). We herein hypothesize that the combined exposure to TWEAK and TGF-β1 may result in the development of steroid resistance in bronchial epithelial cells. The bronchial epithelial cell line BEAS-2B was cultured with or without TGF-β1 or TWEAK, in the presence or absence of dexamethasone (DEX). The roles of Smad-independent pathways and MAP kinase phosphatase 1 (MKP-1) were also explored. Co-stimulation of TWEAK and TGF-β1 induced E-cadherin reduction, N-cadherin upregulation, and TSLP and CCL5 production, which were not suppressed by DEX. Inhibition of the nuclear factor kappa beta (NF-κB) and mitogen-activated protein kinase pathways downregulated steroid-unresponsive TSLP and CCL5 production, whereas knockdown of MKP-1 improved steroid-unresponsive TSLP production, induced by co-stimulation with TWEAK and TGF-β1. Therefore, co-stimulation with TWEAK and TGF-β1 can induce the steroid-insensitive production of TSLP and CCL5 in the bronchial epithelium and may contribute to airway inflammation.
Noggin-Loaded PLA/PCL Patch Inhibits BMP-Initiated Reactive Astrogliosis
Authors: Hawes James , Gonzalez-Manteiga Ana , Murphy Kendall P. , Sanchez-Petidier Marina , Moreno-Manzano Victoria , Pathak Bedika , Lampe Kristin , Lin Chia-Ying , Peiro Jose L. , Oria Marc
Published: October 2024

Myelomeningocele (MMC) is a congenital birth defect of the spine and spinal cord, commonly treated clinically through prenatal or postnatal surgery by repairing the unclosed spinal canal. Having previously developed a PLA/PCL polymer smart patch for this condition, we aim to further expand the potential therapeutic options by providing additional cellular and biochemical support in addition to its mechanical properties. Bone morphogenetic proteins (BMPs) are a large class of secreted factors that serve as modulators of development in multiple organ systems, including the CNS. We hypothesize that our smart patch mitigates the astrogenesis induced, at least partly, by increased BMP activity during MMC. To test this hypothesis, neural stem or precursor cells were isolated from rat fetuses and cultured in the presence of Noggin, an endogenous antagonist of BMP action, with recombinant BMPs. We found that the developed PLA/PCL patch not only serves as a biocompatible material for developing neural stem cells but was also able to act as a carrier for BMP–Notch pathway inhibitor Noggin, effectively minimizing the effect of BMP2 or BMP4 on NPCs cultured with the Noggin-loaded patch.
A Survey of Artificial Intelligence Applications in Nuclear Power Plants
Authors: Jendoubi Chaima , Asad Arghavan
Published: October 2024

Nuclear power plants (NPPs) rely on critical, complex systems that require continuous monitoring to ensure safe operation under both normal and abnormal conditions. Despite the potential of artificial intelligence (AI) to enhance predictive capabilities in these systems, limited research has been conducted on the application of AI algorithms within NPPs. This presents a knowledge gap in the integration of AI for improving safety, reliability, and decision making in NPP. In this study, we explore the use of AI methods, including machine learning and real-time data analytics, applied to NPP components to address the nonlinearity and dynamic behavior inherent in reactor operations. Through the implementation of AI and Internet of Things (IoT) devices, we propose a system that enables early warning and real-time data transmission to regulatory authorities and decision-makers, ensuring better coordination during incidents. Lessons from past nuclear accidents, such as Chernobyl, emphasize the importance of timely information dissemination to mitigate risks. However, this integration also presents challenges, including cybersecurity risks and the need for updated regulations to address AI use in safety-critical environments. The results of this study highlight the urgent need for further research on the application of AI in NPPs, with a particular focus on addressing these challenges to ensure safe implementation.
Open Reduction and Internal Fixation Is a Feasible Alternative to Femoral Revision Arthroplasty in Geriatric Patients with Vancouver B2/3 Type Periprosthetic Fractures: A Study Analyzing In-Hospital Outcomes
Authors: Lampert Christopher , Linhart Christoph , Holzapfel Boris Michael , Böcker Wolfgang , Neuerburg Carl , Zhang Yunjie
Published: October 2024

Purpose: The surgical management of periprosthetic femoral fractures is particularly challenging in geriatric patients due to physiological limitations. The choice between open reduction and internal fixation (ORIF) and hip revision arthroplasty for treating Vancouver B2 and B3 fractures remains controversial. This study aims to contribute further evidence by analyzing the in-hospital outcomes in geriatric patients with Vancouver B2/3 fractures. Methods: This retrospective study analyzed 133 patients treated for Vancouver B2/3 fractures at a level I trauma center from 2017 to 2023. Data were collected on preclinical characteristics, comorbidities, Vancouver classification, surgery-related parameters, and postoperative outcomes for an age- and gender-matched analysis. A subgroup analysis was also conducted on patients classified as American Society of Anesthesiologists (ASA) class 3 and 4. Results: Among the 133 patients, 85 suffered Vancouver B2 fractures and 48 Vancouver B3 fractures. Age-and-gender-matched analysis revealed that ORIF was more commonly performed in patients with higher ASA grades. A subgroup analysis of ASA 3 and 4 patients and an age-and-gender-matched analysis showed that ORIF resulted in shorter operation times and less blood loss. No significant differences were found in mortality or complication rates. Conclusions: ORIF is associated with shorter operation times, less bleeding, and comparable in-hospital outcomes in treating Vancouver B2/3 fractures in higher-risk geriatric patients compared to revision arthroplasty. The retrospective design and small sample size in the ORIF group are limitations of the study. Further studies with functional evaluation are still required.
Selective Digestive Decontamination: A Comprehensive Approach to Reducing Nosocomial Infections and Antimicrobial Resistance in the ICU
Authors: Martínez-Pérez María , Fernández-Fernández Rosario , Morón Rocío , Nieto-Sánchez María Teresa , Yuste María Eugenia , Díaz-Villamarín Xando , Fernández-Varón Emilio , Vázquez-Blanquiño Alberto , Alberola-Romano Ana , Cabeza-Barrera José , Colmenero Manuel
Published: October 2024

Background/Objective: Multidrug-resistant (MDR) bacteria pose a significant threat to global health, especially in intensive care units (ICUs), where high antibiotic consumption drives antimicrobial resistance. Selective digestive decontamination (SDD) is a strategy designed to prevent nosocomial infections and colonization by MDR pathogens. This study aimed to evaluate the impact of implementing an SDD protocol on antibiotic consumption and colonization by carbapenemase-producing Enterobacterale (CPE) in a specific ICU setting. Methods: This quasi-experimental study was conducted in the ICU of a university hospital from June 2021 to June 2023. Patients were divided into two groups: pre-intervention (before SDD) and post-intervention (after SDD implementation). Data on antibiotic consumption (expressed as defined daily doses (DDDs) per 100 stays), nosocomial infections, colonization rates, and the incidence of MDR bacteria were collected. A statistical analysis was conducted to compare the pre- and post-intervention groups. Results: A total of 3266 patients were included, with 1532 in the pre-intervention group and 1734 in the post-intervention group. The implementation of the SDD protocol resulted in a significant reduction in total antibiotic consumption (p = 0.028), with notable decreases in carbapenem use (p < 0.01) and colonization by CPE (p = 0.0099). The incidence of nosocomial infections also decreased in the post-SDD group, although this reduction was not statistically significant. Conclusions: The implementation of the SDD protocol in this ICU setting significantly reduced antibiotic consumption and colonization by CPE. These findings suggest that SDD may be a valuable tool in managing antimicrobial resistance in critical care settings, without contributing to the development of MDR bacteria.
Radiomics for Predicting Prognostic Factors in Breast Cancer: Insights from Contrast-Enhanced Mammography (CEM)
Authors: Piccolo Claudia Lucia , Sarli Marina , Pileri Matteo , Tommasiello Manuela , Rofena Aurora , Guarrasi Valerio , Soda Paolo , Beomonte Zobel Bruno
Published: October 2024

Objectives: To evaluate the correlation between radiomic features extracted from contrast-enhanced mammography (CEM) tumor lesions and peritumoral background with prognostic factors in breast cancer (BC). Methods: In this retrospective, single-center study, 134 women with histologically confirmed breast cancer underwent CEM examination. Radiomic features were extracted from manually segmented lesions and lesion contours were automatically delineated using PyRadiomics. The extracted features were categorized into seven classes: First-order Features, Shape Features (2D), Gray Level Co-occurrence Matrix (GLCM), Gray Level Run Length Matrix (GLRLM), Gray Level Size Zone Matrix (GLSZM), and Neighboring Gray Tone Difference Matrix (NGTDM). Histological examination assessed tumor type, grade, receptor structure (ER, PgR, HER2), Ki67 index, and lymph node involvement. Pearson correlation and multivariate regression were applied to evaluate associations between radiomic features and prognostic factors. Results: Significant correlations were found between First-order Features and prognostic factors such as ER, PgR, and Ki67 (p < 0.05). GLCM-based texture features showed strong associations with Ki67 and HER2 (p < 0.01). Radiomic features from peritumoral regions, especially shape and GLSZM metrics, were significantly correlated with Ki67 and lymph node involvement. Conclusions: Radiomic analysis of both tumor and peritumoral regions offers significant insights into BC prognosis. These findings support the integration of radiomics into personalized diagnostic and therapeutic strategies, potentially improving clinical decision making in BC management.
Corticospinal Tract Sparing in Cervical Spinal Cord Injury
Authors: Schuch Clarissa Pedrini , Jovanovic Lazar I. , Balbinot Gustavo
Published: October 2024

Disruptions in the brain’s connections to the hands resulting from a cervical spinal cord injury (cSCI) can lead to severe and persistent functional impairments. The integrity of these connections is an important predictor of upper extremity recovery in stroke and may similarly act as a biomarker in cSCI. In this perspective article, we review recent findings from a large cohort of individuals with cSCI, demonstrating the predictive value of corticospinal tract (CST) integrity in cSCI—CST sparing. This research underscores that, akin to stroke, the integrity of brain-to-hand connections is crucial for predicting upper extremity recovery following cSCI. We address the limitations of commonly used metrics, such as sacral sparing and the concept of central cord syndrome. Furthermore, we offer insights on emerging metrics, such as tissue bridges, emphasizing their potential in assessing the integrity of brain connections to the spinal cord.
Exploring the Spatiotemporal Heterogeneities in Urban Vitality Through Scalable Proxies from Mobile Data
Authors: Park Sunggyun , Lee Dongwoo
Published: October 2024

Jane Jacob’s concepts of urban vitality and diversity have become prevailing urban planning philosophies in most countries for making cities more livable. Recent changes in demographics and the impacts of COVID-19 have exacerbated the economic and social challenges that cities commonly face, particularly spatiotemporal heterogeneities. Being able to understand these heterogeneities in scalable approaches is fundamental to tackling these challenges in cities. Therefore, this article aims to provide a new form of scalable estimation of urban vitality by using the de facto population. Instead of merely adopting static statistical information such as morphological characteristics in areas, we leverage dynamic factors such as internal mobility flows and energy use intensity as proxies for the spatiotemporal dynamics of indoor and outdoor behaviors of crowds. In this way, we combine dynamic attributes and static features to describe the patterns of urban vitality, which are directly related to spatiotemporal dynamics in urban places. We utilize GNSS-based mobile data and building energy usage intensity as dynamic proxies along with static data such as land use mix and age distribution. To better capture spatial heterogeneity, we use a Multiscale Geographically Weighted Regression (MGWR) model to identify the relationships between the de facto population and the dynamic and static factors. Drawing from the factors determining urban vitality, this article provides policy implications for alleviating spatiotemporal urban imbalances. These data-driven implications can fill the technical knowledge gaps in establishing planning strategies for achieving urban sustainability while enhancing overall subjective livability.
Enterotype-Specific Effects of Red Beetroot (Beta vulgaris L.) Powder and Betanin on Human Gut Microbiota: A Preliminary Study Based on In Vitro Fecal Fermentation Model
Authors: Ko Gwang-Pyo , Jo Hyejun , Kim Jungman , Kim Jeong Seon , Boo Kyung-Hwan , Kim Chang Sook
Published: October 2024

Red beetroots, rich in betanin, may act as prebiotics and impact gut microbiota. Because the human gut microbiota is unique to each person, the effectiveness of prebiotics varies with the enterotype. In this study, we hypothesized that the effects of red beetroot powder (RP) and betanin pigment (BP) would differ depending on the enterotype. Fecal samples from 30 subjects were analyzed and categorized into three enterotypes: Phocaeicola, Prevotella, and Bifidobacterium. Feces were collected from one representative subject from each enterotype cluster for fermentation. Results showed that RP and BP affected microbiota composition and short-chain fatty acid (SCFA) production differently across enterotypes. The Bifidobacterium cluster showed significantly reduced alpha diversity, with the direction of change in the gut microbiota composition being different from that of other subjects. Additionally, SCFAs significantly increased, with the highest increase in the Bifidobacterium cluster. In this cluster, metabolic pathways related to SCFAs (i.e., starch and sucrose metabolism and glycolysis/gluconeogenesis) were altered. Conversely, Prevotella-dominant feces exhibited fewer changes in SCFAs and a lower increase in Bifidobacterium abundance than the others. These findings highlight that RP and BP elicit enterotype-specific responses in the gut microbiota composition and SCFA production, emphasizing the importance of enterotypes in personalized nutrition.
Sports Medicine: Nutritional Sciences and Nutritional Biochemistry, Focusing on Nutritional Supplements from the FFNFO Framework to Contamination
Authors: Bonilla Diego A. , Petro Jorge L. , Kreider Richard B. , Cannataro Roberto
Published: October 2024

Sports medicine has become a well-established field with a wide range of applications, from competitive and professional athletics to general well-being [...]
Orthodontic Alloy Wires and Their Hypoallergenic Alternatives: Metal Ions Release in pH 6.6 and pH 5.5 Artificial Saliva
Authors: Jusufi Osmani Zana , Tariba Knežević Petra , Vučinić Davor , Alimani Jakupi Jetmire , Reka Arianit A. , Can Mustafa , Kara Koray , Katić Višnja
Published: October 2024

Legislative framework addresses the issues of alloy corrosion, demanding the restricted use of probable carcinogenic, mutagenic, and toxic-for-human-reproduction (CMG) metals like nickel, cobalt, and chromium and demanding the development of new biomaterials. The aim of this research was to evaluate and compare the ion release of standard dental alloys and their hypoallergenic equivalents. Six types of orthodontic alloy wires (nickel–titanium (NiTi), coated NiTi, stainless steel (SS), Ni-free SS, and cobalt–chromium (CoCr) and titanium–molybdenum (TMA) were immersed into artificial saliva of pH 5.5 and 6.6. Release of metal ions was measured by inductively coupled plasma–mass spectrometry after 3, 7, 14 and 28 days. The data were analyzed using analysis of variance, and results with p < 0.05 were considered significant. NiTi released more Ti and Ni ions compared to the coated NiTi; SS released more iron, chromium, and nickel compared to the nickel-free SS. CoCr released cobalt in a high concentration and low amounts of chromium, nickel, and molybdenum compared to the molybdenum and titanium released by TMA. Release of metals from dental orthodontic alloys in vitro was overall lower at pH 6.6 and for the hypoallergenic equivalents when compared to standard dental alloys.
Better Ce (III) Sorption Properties of Unprocessed Chitinous Waste from Hermetia illucens than Commercial Chitosans
Authors: Bąk Justyna , Bulak Piotr , Kaczor Monika , Kołodyńska Dorota , Bieganowski Andrzej
Published: October 2024

Insect farming generates a new type of chitinous waste in the form of dead specimens that have died of natural causes and insect moults (puparia), particularly large amounts of which are generated during the rearing of holometabolous insects. Following the circular economy paradigm, we treated waste in the form of puparia and dead adults of H. illucens as a valuable material, i.e., as sources of chitin, and tested it as a sorbent for cerium, a lanthanide of great industrial importance. For comparison, non-treated, raw insect materials and commercial chitosans were also investigated. Chitin extracted from H. illucens showed an adsorption capacity at the same level as commercially available, marine-source chitin (approximately 6 mg Ce·g−1). However, more interestingly, raw materials exhibited much higher adsorption capacities—dead adults were similar to commercial chitosans (approximately 32 mg Ce·g−1), while puparia demonstrated twice the performance (approximately 60 mg Ce·g−1). This indicates that unprocessed waste can be used as environmentally friendly, cost-effective Ce biosorbents with comparable or even better sorption capacity than chitosans, whose production requires intense chemical processing.
Advances in Nanostructured Metallic Materials—A Pathway to Future Innovations
Authors: Wojnicki Marek , Escribà-Gelonch Marc , Hessel Volker
Published: October 2024

The development of civilization has always been deeply intertwined with advancements in metallurgy [...]
Design and Optimization of a Fan-Out Wafer-Level Packaging- Based Integrated Passive Device Structure for FMCW Radar Applications
Authors: Yang Jiajie , Xu Lixin , Yang Ke
Published: October 2024

This paper presents an integrated passive device (IPD) structure based on fan-out wafer-level packaging (FOWLP) for the front end of frequency-modulated continuous wave (FMCW) radar systems, focusing on enhancing the integration efficiency and performance of large passive components like antennas. Additionally, a new metric is introduced to assess this structure’s effect on the average noise figure in FMCW systems. Using this metric as a loss function, we apply the support vector machine (SVM) for electromagnetic simulation and the genetic algorithm (GA) for optimization. The sample fitting variance is 2.42 dB, reducing computation time from 12 min to under 1 millisecond, with the entire optimization completed in less than 100 s. The optimized IPD structure is 0.7 × 0.9 × 0.014 λ03 in size and achieves over 35 dB isolation between the transmitter and receiver. Compared to the IPD model calculated by empirical formulas, the optimized device lowers the average noise figure by 15.2 dB and increases maximum gain by 4.19 dB.
One-Step Fabrication of 2.5D CuMoOx Interdigital Microelectrodes Using Numerically Controlled Electric Discharge Machining for Coplanar Micro-Supercapacitors
Authors: Yang Shunqi , Chen Ri , Huang Fu , Wang Wenxia , Zhitomirsky Igor
Published: October 2024

With the increasing market demands for wearable and portable electronic devices, binary metal oxides (BMOs) with a remarkable capacity and good structure stability have been considered as a promising candidate for fabricating coplanar micro-supercapacitors (CMSCs), serving as the power source. However, the current fabrication methods for BMO microelectrodes are complex, which greatly hinder their further development and application in BMO CMSCs. Herein, the one-step fabrication of 2.5D CuMoOx-based CMSCs (CuMoCMSCs) has been realized by numerically controlled electric discharge machining (NCEDM) for the first time. In addition, the controllable capacity of CuMoCMSCs has been achieved by adjusting the NCEDM-machining voltage. The CuMoCMSCs machined by a machining voltage of 60 V (CuMoCMSCs60) showed the best performance. The fabricated CuMoCMSCs60 with binary metal oxides could operate at an ultra-high scanning rate of 10 V s−1, and gained a capacity of 40.3 mF cm−2 (1.1 mA cm−2), which is more than 4 times higher than that of MoOx-based CMSCs (MoCMSCs60) with a single metal oxide. This is because CuMoOx BMOs materials overcome the poor electroconductivity problem of the MoOx single metal oxide. This one-step and numerically controlled fabrication technique developed in this research opens a new vision for preparing BMO materials, BMO microelectrodes, and BMO microdevices in an environmental, automatic, and intelligent way.
Strategies for Hydrocarbon Removal and Bioleaching-Driven Metal Recovery from Oil Sand Tailings
Authors: Joshi Khyati , Magdouli Sara , Kaur Kamalpreet , Brar Satinder Kaur
Published: October 2024

Oil sand tailings from bitumen extraction contain various contaminants, including polycyclic aromatic hydrocarbons, BTEX, and naphthenic acids, which can leak into surrounding environments, threatening aquatic ecosystems and human health. These tailings also contribute to environmental issues such as habitat disruption and greenhouse gas emissions. Despite these challenges, oil sand tailings hold significant potential for waste-to-resource recovery as they contain valuable minerals like rare earth elements (REEs), titanium, nickel, and vanadium. Traditional metal extraction methods are environmentally damaging, requiring high energy inputs and generating dust and harmful emissions. Furthermore, the coating of hydrocarbons on mineral surfaces presents an additional challenge, as it can inhibit the efficiency of metal extraction processes by blocking access to the minerals. This highlights the need for alternative, eco-friendly approaches. Bioleaching, which uses microorganisms to extract metals, emerges as a sustainable solution to unlock the valuable metals within oil sand tailings. This review discusses the minerals found in oil sand tailings, the challenges associated with their extraction, methods from hydrocarbon removal from minerals, and bioleaching as a potential metal recovery method.
The Application of the Supervised Descent Method in the Inversion of 3D Direct Current Resistivity Data
Authors: Wan Tingli , Li Tonglin , Kang Xinze , Zhang Rongzhe
Published: October 2024

Three-dimensional direct current resistivity inversion is vital for mineral exploration, offering detailed electrical property distribution data of subsurface resources. To overcome the traditional algorithm’s reliance on initial models, its tendency to become stuck in local optima, and its low inversion resolution, this paper introduces an SDM-based 3D direct current resistivity inversion method. The Supervised Descent Method (SDM) is primarily used to solve optimization problems in nonlinear least squares, effectively capturing subsurface structural details and identifying electrical anomalies through the integration of machine learning and gradient descent techniques, thereby precisely revealing the complex electrical characteristics underground. Moreover, this study incorporates smooth regularization to enhance the stability and reliability of the inversion results. The paper demonstrates the feasibility and generalization of the SDM in 3D resistivity inversion through two sets of model calculations. A comparative analysis with traditional methods further proves the advantages of the SDM algorithm in improving inversion resolution and efficiency. Finally, applying the SDM algorithm to the Xiagalaoyi River mining area in Heilongjiang Province fully proves its optimized data processing capabilities and sensitivity to complex geological structures, providing a more precise and rapid technical approach for mineral resource exploration and assessment.
Tripodal Quinone-Cyanine G-Quadruplex Ligands as Novel Photosensitizers on Photoinduced Cancer Cell Death
Authors: Muramoto Junya , Sakamoto Takashi
Published: October 2024

Guanine-quadruplex (G4) selective photosensitizers have huge potential for photodynamic therapy against various diseases correlated with G4 DNA and G4 RNAs; however, the types of photosensitizer skeletons available are limited. Herein, we investigated the ability of our original G4 ligands, tripodal quinone-cyanine dyes (tpQCy(s)), which were developed as fluorescent probes for G4, to act as photosensitizers for cancer-selective apoptosis inducers. The results indicated that the tpQCy skeleton has great potential for developing G4-targeted cancer-selective photosensitizers for photodynamic therapy. Among the two tpQCys, only QCy(BnBT)3, which has greater G4 selectivity, exhibited photoinduced cytotoxicity in HeLa cell growth, suggesting that the direct oxidation of G4 DNA or RNA is crucial for photoinduced cytotoxicity. RNA-seq analysis using a next-generation sequencing technique revealed that apoptosis was clearly induced by photoirradiation after QCy(BnBT)3 treatment.
Visual Detection of Ascorbic Acid via Smartphone with Portable Probe Based on Assembled FePO4@GO Peroxidase-like Nanozyme
Authors: Zhang Zifang , Bai Junhua , Gong Yan , Zhang Lifang
Published: October 2024

Ascorbic acid (AA) plays an important role in many physiological and biochemical processes. Adequate quantification of AA is required for controlling the intake of AA for health management. In this study, a portable probe was fabricated based on the assembled FePO4@GO nanozyme by homogeneous precipitation. FePO4@GO exhibits excellent peroxidase (POD) activity. As an efficient free radical scavenger, ascorbic acid scavenges 1O2 free radicals produced by hydrogen peroxide catalyzed by peroxidase enzymes and then inhibits the color reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). A good linear relationship between the color of the reaction system and the concentration of AA in the range of 2.5–75 μM was presented, and the detection limit is 1.25 μM. Furthermore, a visual colorimetric detection platform was constructed using a smartphone. The AA content is accurately reflected by intelligent recognition of color changes in the reaction system. It provides an alternative approach for portable and visual rapid detection of Vitamin C (VC) in medicine. Meanwhile, it also provides a reference for the assembly of an intelligent detection platform based on nanozymes.
Experimental Polarizability Transition Moments of CO2 for Excited Vibrational States
Authors: Álvarez Carlos , Tejeda Guzmán , Fernández José M.
Published: October 2024

The Raman spectrum of CO2 from room temperature to 1800 K has been measured in a series of experiments. The differential Raman scattering cross-sections for the fundamental bands at 1285.41 cm−1 and 1388.18 cm−1 have been obtained from reference bands of H2 and N2 as intensity standards. The Raman cross-sections of CO2 hot bands, involving vibrational energy levels up to 5000 cm−1, were derived from those of the fundamental bands. The Raman cross-sections obtained this way were reduced to transition moments of the mean molecular polarizability, which make it possible to simulate the Raman spectrum of CO2 up to 2000 K. This paves the way for local or remote diagnostics of CO2 in hot environments using Raman based techniques.
An MEDT Study of the Reaction Mechanism and Selectivity of the Hetero-Diels–Alder Reaction Between 3-Methylene-2,4-Chromandione and Methyl Vinyl Ether
Authors: Bouhaoui Abderrazzak , Moumad Aziz , Domingo Luis R. , Bouissane Latifa
Published: October 2024

The hetero-Diels–Alder (HDA) reaction between the ambident heterodiene 3-methylene-2,4-chromandione (MCDO) and non-symmetric methyl vinyl ether (MVE) is investigated using the molecular electron density theory (MEDT) at the B3LYP/6-311G(d,p) computational level. The aim of this study is to gain insight into its molecular mechanism and to elucidate the factors that control the selectivity found experimentally. DFT-based reactivity indices reveal that MCDO exhibits strong electrophilic characteristics, while MVE displays a strong nucleophilic character. Meanwhile, the Parr function explains the ortho regioselectivity of this HDA reaction. The highly polar nature of this HDA reaction, supported by the high global electron density transfer (GEDT) taking place at the transition state structures (TSs), accounts for the very low activation energy associated with the most favorable TS-4on. The ambident nature of MCDO allows for the formation of two constitutional isomeric cycloadducts. In the case of MVE, pseudocyclic selectivity is attained using a thermodynamic control. This polar HDA reaction displays an endo stereoselectivity and a complete ortho regioselectivity. A comparative relative interacting atomic energy (RIAE) analysis of the two diastereomeric structures TS-4on and TS-6on indicates a high degree of likeness, which explains the low pseudocyclic selectivity under kinetic control.
Preparation and Luminescence Property Study of Red-Emitting Na3.6Y1.8(PO4)3:Eu3+,Li+/K+ Phosphors with Excellent Thermal Stability for Light-Conversion Application
Authors: You Qi , Zhou Xuan , Yang Chengxiang , Liu Mu , Liu Wei , Li Jinkai , Jiang Xuchuan
Published: October 2024

A series of red-emitting phosphors, Na3.6Y1.8−x(PO4)3:xEu3+, have been synthesized by a high-temperature solid-phase method. The impact of the partial Li+/K+ ion substitution on the crystal structure and photoluminescence (PL) performance of Na3.6Y1.05(PO4)3:0.75Eu3+ phosphor have been investigated. Various techniques have been used for characterization of the as-obtained materials. X-ray diffraction (XRD) analysis was utilized to confirm the composites of these samples, and the morphology and element distribution were examined by scanning electron microscope (SEM) and transmission electron microscope (TEM). This study found that the developed Na3.6Y1.8−x(PO4)3:xEu3+ phosphors exhibited a prominent emission peak at ~620 nm when excited at 393 nm, which corresponded to 5D0 → 7F2 transitions of Eu3+ ions. Furthermore, the robust emission peak at ~705 nm (5D0 → 7F4) of these phosphors enables a better match with plant pigment absorption. Beyond that, the partial substitution of Li+/K+ ions probably changed the crystal structure, and reduces the symmetry around Eu3+, leading to significantly enhanced luminous intensities by 23.24% and 18.29%, with the highest quantum yields (QYs) reaching 99.85% and 96.29%, respectively. Additionally, the prepared phosphors show non-thermal quenching and superior thermal stability at elevated temperatures from 298 to 473 K. These findings and results suggest that Li⁺/K⁺-substituted Na3.6Y1.05(PO₄)₃:0.75Eu3⁺ phosphors can serve as promising red-emitting phosphors for plant lighting applications.
Diet and Disease Development: Mechanisms, Prevention, and Treatment
Authors: Saad Sonia , Chen Hui
Published: October 2024

The overall aims of this Special Issue “Diet and Disease Development: Mechanisms, Prevention and Treatment” are to describe and emphasise the importance of diet in disease development; understand the mechanism(s) whereby an unhealthy diet can induce various diseases; examine the potential effects of natural compounds or extracts on disease prevention or treatment; and determine whether nutrients and their metabolites can be used as biomarkers to diagnose certain diseases [...]
DNA Barcoding Using 18S rRNA Gene Fragments for Identification of Tick-Borne Protists in Ticks in the Republic of Korea
Authors: Alkathiri Badriah , Lee Subin , Ahn KyuSung , Youn So Youn , Yoo Mi-Sun , Lee Hyang-Sim , Cho Yun Sang , Jung Jaeyun , Seo Kwangwon , Kim Soochong , Umemiya-Shirafuji Rika , Xuan Xuenan , Kwak Dongmi , Shin SungShik , Lee Seung-Hun
Published: October 2024

The objective of this study was to evaluate the diversity and prevalence of tick-borne protists in the Republic of Korea via DNA barcoding using 18S rRNA gene fragments and PCR. Between 2021 and 2022, questing ticks were collected using the flagging method, with a total of 13,375 ticks collected and pooled into 1003 samples. Of these, 50 tick pools were selected for DNA barcoding targeting the V4 and V9 regions of 18S rRNA using the MiSeq platform. A taxonomic analysis of the amplicon sequence variants identified three genera of protozoa, namely Hepatozoon canis, Theileria luwenshuni, and Gregarine sp. However, the number and abundance of protists detected were different depending on the primer sets, and T. gondii was not identified in DNA barcoding. Furthermore, conventional PCR confirmed the presence of H. canis, Toxoplasma gondii, T. luwenshuni, and Theileria sp. in the collected ticks. This study identified H. canis and T. gondii in Ixodes nipponensis for the first time. It demonstrated that the results of DNA barcoding using 18S rRNA gene fragments can vary depending on the primer sets and further optimization is required for library construction to identify tick-borne protists in ticks. Despite these limitations, the findings highlight the potential of DNA barcoding using 18S rRNA gene fragments for screening the diversity of tick-borne protists in ticks.