bluuebunny/crossref_metadata_2025 huggingface dataset:
Using Automated Vehicle Technologies With Older Adults: A Mixed-Methods Study
Authors: Isbel Stephen , Mulhall Stephanie , Gibson Diane
Published: March 2022

When older adults’ driving abilities decline, automated driving technologies may improve community mobility, engagement, and independence. Most previous research has focused on older persons’ attitudes rather than their use of automated driving technologies. This study examined older Australians’ perceptions and experience of automated vehicle technologies before, during, and after a real-life driving experience, focusing on ease of use, usefulness, safety, acceptance, trust, and confidence. This mixed-methods study included observation of a 6-km test drive using a partially automated vehicle, pre- and post-drive questionnaires, and a post-drive semi-structured interview. Most participants reported positive perceptions and experiences before, during, and after the test drive. Visual analysis of pre/postresponses revealed divergent reactions to the test drive, consistent with the heterogeneity of the older population. Automated driving technologies have potential to contribute to mobility at older ages. Larger-scale studies including actual driving experiences are recommended.
A glimpse into the future of systemic lupus erythematosus
Authors: Aringer Martin , Alarcón-Riquelme Marta E. , Clowse Megan , Pons-Estel Guillermo J. , Vital Edward M. , Dall’Era Maria
Published: March 2022

This viewpoint article on a forecast of clinically meaningful changes in the management of systemic lupus erythematosus (SLE) in the next 10 years is based on a review of the current state of the art. The groundwork has been laid by a robust series of classification criteria and treatment recommendations that have all been published since 2019. Building on this strong foundation, SLE management predictably will take significant steps forward. Assessment for lupus arthritis will presumably include musculoskeletal sonography. Large-scale polyomics studies are likely to unravel more of the central immune mechanisms of the disease. Biomarkers predictive of therapeutic success may enter the field; the type I interferon signature, as a companion for use of anifrolumab, an antibody against the common type I interferon receptor, is one serious candidate. Besides anifrolumab for nonrenal SLE and the new calcineurin inhibitor voclosporin in lupus nephritis, both of which are already approved in the United States and likely to become available in the European Union in 2022, several other approaches are in advanced clinical trials. These include advanced B cell depletion, inhibition of costimulation via CD40 and CD40 ligand (CD40L), and Janus kinase 1 (Jak1) and Tyrosine kinase 2 (Tyk2) inhibition. At the same time, essentially all of our conventional therapeutic armamentarium will continue to be used. The ability of patients to have successful SLE pregnancies, which has become much better in the last decades, should further improve, with approaches including tumor necrosis factor blockade and self-monitoring of fetal heart rates. While we hope that the COVID-19 pandemic will soon be controlled, it has highlighted the risk of severe viral infections in SLE, with increased risk tied to certain therapies. Although there are some data that a cure might be achievable, this likely will remain a challenge beyond 10 years from now.
Increased risks of aortic regurgitation and atrial fibrillation in radiographic axial spondyloarthritis patients: a 10-year nationwide cohort study
Authors: Min Hong Ki , Kim Hae-Rim , Lee Sang-Heon , Park Sojeong , Park Minae , Hong Yeon Sik , Kim Moon-Young , Park Sung-Hwan , Kang Kwi Young
Published: March 2022

Background:

To compare the incidences of aortic regurgitation, atrial fibrillation (AF), and atrioventricular (AV) block II–III between radiographic axial spondyloarthritis (r-axSpA) patients and the general population (GP).

Methods:

National Health Insurance Services data were used. R-axSpA patients ( N = 8877) and the age- and sex-matched GP ( N = 26,631) were followed from August 2006 to December 2019. Incidence rates and standardized incidence ratios (SIRs) of aortic regurgitation, AF, and AV block II–III were compared between these groups. Ten-year incidence rates and hazard ratios (HRs) were calculated by the Kaplan–Meier method and Cox regression analysis.

Results:

Incidence rates of aortic regurgitation, AV block II–III, and AF in the r-axSpA group were 0.42, 0.21, and 4.0 per 1000 person-years (PYs), respectively. In the r-axSpA group, the SIR for aortic regurgitation was highest among 40- to 49-year-old men (4.11). Incidence rates of aortic regurgitation and AF were higher in the r-axSpA group than in the GP group (0.42 versus 0.18 per 1000 PYs 4.00 versus 3.13 per 1000 PYs, both p < 0.001, respectively), whereas the difference was insignificant for AV block II–III (0.21 versus 0.14 per 1000 PYs, p = 0.222). In multivariate analysis, r-axSpA was associated with a higher hazard (risk) for the development of aortic regurgitation and AF [HR (95% confidence interval) = 2.55 (1.49–4.37) and 1.20 (1.04–1.39), respectively], but the difference was insignificant for AV block II–III [HR (95% confidence interval) = 1.17 (0.59–2.31)].

Conclusions:

Compared with the GP, r-axSpA patients are at increased risk of aortic regurgitation and AF, but not AV block II–III. These patients should be carefully monitored for occurrence of aortic regurgitation and AF.
Inverted intercostal hernia of elastofibroma dorsi mimicking well-differentiated liposarcoma in the chest wall
Authors: Yoshida Rika , Yoshizako Takeshi , Okamura Kazuya , Ando Shinji , Nakamura Megumi , Ishikawa Noriyoshi , Kitagaki Hajime
Published: March 2022

Elastofibroma dorsi is a well-known benign chest wall tumor. Herein, we present a case in which an elastofibroma protruded into the thoracic cavity, leading to inverted intercostal hernia. Imaging revealed a soft tissue mass containing fat, typical of elastofibroma dorsi; however, precise diagnosis was difficult owing to the location of this mass that protruded into the thoracic cavity. Liposarcoma had to be ruled out because it was a growing fat-containing mass. Considering that the tumor moved while the patient was undergoing computed tomography-guided biopsy in the prone position, a diagnosis of inverted intercostal hernia of elastofibroma dorsi was made. We report this case with a review of current literature.
Impact of gene annotation choice on the quantification of RNA-seq data
Authors: Chisanga David , Liao Yang , Shi Wei
Published: March 2022

Background

RNA sequencing is currently the method of choice for genome-wide profiling of gene expression. A popular approach to quantify expression levels of genes from RNA-seq data is to map reads to a reference genome and then count mapped reads to each gene. Gene annotation data, which include chromosomal coordinates of exons for tens of thousands of genes, are required for this quantification process. There are several major sources of gene annotations that can be used for quantification, such as Ensembl and RefSeq databases. However, there is very little understanding of the effect that the choice of annotation has on the accuracy of gene expression quantification in an RNA-seq analysis.

Results

In this paper, we present results from our comparison of Ensembl and RefSeq human annotations on their impact on gene expression quantification using a benchmark RNA-seq dataset generated by the SEQC consortium. We show that the use of RefSeq gene annotation models led to better quantification accuracy, based on the correlation with ground truths including expression data from >800 real-time PCR validated genes, known titration ratios of gene expression and microarray expression data. We also found that the recent expansion of the RefSeq annotation has led to a decrease in its annotation accuracy. Finally, we demonstrated that the RNA-seq quantification differences observed between different annotations were not affected by the use of different normalization methods.

Conclusion

In conclusion, our study found that the use of the conservative RefSeq gene annotation yields better RNA-seq quantification results than the more comprehensive Ensembl annotation. We also found that, surprisingly, the recent expansion of the RefSeq database, which was primarily driven by the incorporation of sequencing data into the gene annotation process, resulted in a reduction in the accuracy of RNA-seq quantification.
Determinants of birth asphyxia among newborns in Debre Berhan referral hospital, Debre Berhan, Ethiopia: a case-control study
Authors: Tegegnework Sisay Shine , Gebre Yeshfanos Tekola , Ahmed Sindew Mahmud , Tewachew Abrham Shitaw
Published: March 2022

Background

Birth asphyxia is the major public health problem in the world. It is estimated that around 23% of all newborn deaths are caused by birth asphyxia worldwide. Birth asphyxia is the top three causes of newborn deaths in sub-Saharan Africa and more than one-third of deaths in Ethiopia. Therefore, the aim of this study was to identify determinants of birth asphyxia which can play a crucial role to decrease the death of newborns.

Methods

Unmatched case-control study design was implemented among 276 (92 cases and 184 controls) newborns from January 1st to March 30th, 2020. A systematic sampling technique was used to select the study participants. Data were collected by using a semi-structured interviewer-administered questionnaire and document review by trained nurses and midwives who work at the delivery ward of the hospitals. Bivariate logistic regression analysis was done to identify determinants of birth asphyxia. Adjusted odds ratios with 95% confidence intervals and p-value less than and equal to 0.05 were used to assess the level of significance.

Results

In this study, maternal education of being can’t read & write [AOR = 4.7, 95% CI: (1.2, 11.9)], ante-partum hemorrhage [AOR = 7.7, 95% CI: (1.5, 18.5)], prolonged labor [AOR =13.5, 95% CI: (2.0, 19.4)], meconium stained amniotic fluid [AOR = 11.3, 95% CI: (2.7, 39.5)], breech fetal presentation [AOR = 4.5, 95% CI: (2.0, 8.4)] and preterm birth [AOR: 4.1, 95% CI: (1.8, 9.2)] were factors which showed significantly associated with birth asphyxia among newborns.

Conclusions

In this study, maternal education can’t read & write, antepartum hemorrhage, prolonged labor, stained amniotic fluid, breech fetal presentation, preterm birth were significantly associated with birth asphyxia. So, educating mothers to enhance health-seeking behaviors and close monitoring of the labor and fetus presentation were recommended to reduce birth asphyxia.
The impact of new government childcare accreditation standards on children’s in-care physical activity and sedentary time
Authors: Carson Valerie , Zhang Zhiguang , Kuzik Nicholas , Adamo Kristi B. , Predy Madison , Crozier Mitchell , Hunter Stephen , Ogden Nancy , Goldfield Gary S. , Okely Anthony D.
Published: March 2022

Background

A new physical activity and sedentary behaviour accreditation standard criterion for childcare settings was introduced by the provincial government in Alberta, Canada. The primary objective of this study was to examine if changes for in-care physical activity and sedentary time (ST) differed between centres in and around Edmonton, Alberta after implementing the new accreditation standards and non-accredited control centres in and around Ottawa, Ontario. Secondary objectives were to examine whether baseline age group (toddler, preschooler) or the childcare environment moderated any group differences in change of the primary outcomes. Furthermore, accreditation and control group differences in change of children’s body mass index (BMI) Z-scores or cognitive development as well as educators’ perceptions of the primary outcomes were explored.

Methods

Participants were 252 toddlers (19–35 months) and preschoolers (36–60 months) in childcare centres from Alberta (n = 11) and Ontario (n = 8) in the supporting Healthy physical AcTive CHildcare setting (HATCH) study. In-care ST, light-intensity physical activity (LPA), and moderate- to vigorous-intensity physical activity (MVPA) were accelerometer–derived before and 6 months after the implementation of the new standards. At both time points, cognitive development (working memory, expressive vocabulary), heights, and weights were measured, and BMI Z-scores were calculated. Additionally, the childcare environment was observed using the Environment and Policy Assessment and Observation (EPAO) and Movement Environment Rating Scale (MOVERS) tools. Demographic characteristics were parent-reported and weather variables were derived from Environment Canada data. Mixed models were conducted.

Results

In adjusted models (n = 241), change in children’s in-care ST (B = -0.07, 95%CI: − 1.43,1.29), LPA (B = 0.08, 95%CI: − 0.89,1.05), and log–transformed MVPA (B = 0.01, 95%CI: − 0.09,0.11) were not significantly different between accreditation and control groups. Age group and the childcare environment were not moderators. Significant increases in BMI Z-score (B = 0.19, 95%CI: 0.03,0.35) and high working memory (OR = 3.24, 95%CI: 1.32,7.97) were observed in the accreditation group and significant increases in expressive vocabulary (B = 3.18, 95%CI: 0.05,6.30) were observed in the control group.

Conclusions

The new accreditation criterion may not significantly change physical activity or ST in childcare settings and therefore may not explain findings for BMI Z-scores and cognitive development. Additional training and resources may be needed.
Views of the public about Snacktivity™: a small changes approach to promoting physical activity and reducing sedentary behaviour
Authors: Gokal K. , Amos-Hirst R. , Moakes C. A. , Sanders J. P. , Esliger D. W. , Sherar L. B. , Ives N. , Biddle S. J. H. , Edwardson C. , Yates T. , Frew E. , Greaves C. , Greenfield S. M. , Jolly K. , Skrybant M. , Maddison R. , Mutrie N. , Parretti H. M. , Daley A. J.
Published: March 2022

Background

Many people do not meet the recommended health guidance of participation in a minimum of 150–300 min of moderate intensity physical activity per week, often promoted as at least 30 min of physical activity on 5 days of the week. This is concerning and highlights the importance of finding innovative ways to help people to be physically active each day. Snacktivity™ is a novel approach that aims to encourage people to do small, 2–5 min bouts of physical activity ‘snacks’ throughout the whole day, such that they achieve at least 150 min of moderate intensity activity per week. However, before it can be recommended, there is a need to explore whether the concept is acceptable to the public.

Methods

A survey to assess the views of the public about Snacktivity™ was distributed to adult patients registered at six general practices in the West Midlands, UK and to health care employees in the same region.

Results

A total of 5989 surveys were sent to patients, of which 558 were returned (9.3%). A further 166 surveys were completed by health care employees. A total of 85% of respondents liked the Snacktivity™ concept. The flexibility of the approach was highly rated. A high proportion of participants (61%) reported that the ability to self-monitor their behaviour would help them to do Snacktivity™ throughout their day. Physically inactive participants perceived that Snacktivity™ would help to increase their physical activity, more than those who were physically active (OR = 0.41, 95% CI: 0.25–0.67). Approximately 90% of respondents perceived that Snacktivity™ was easy to do on a non-working day compared to 60% on a working day. Aerobic activity ‘snacks’ were preferred to those which were strength based.

Conclusions

The Snacktivity™ approach to promoting physical activity was viewed positively by the public and interventions to test the merits of such an approach now need to be developed and tested in a variety of everyday contexts.
Success rate of fractured teeth receiving modified crown lengthening surgery and restorations
Authors: Wang Cui , Jia Xue-ting , Zhen Min , Hu Wen-Jie , Zhang Hao , Chung Kwok-Hung
Published: March 2022

Background

Whether to preserve a structurally compromised tooth or remove it is a dilemma often encountered by clinicians. The aim of this study was to assess the long-term success rate of fractured teeth preserved by modified crown lengthening surgery and restorations.

Methods

Thirty-nine patients with a total of 45 fractured teeth who had received modified crown lengthening surgery were recruited and examined. Numbers of teeth lost were recorded, and the criteria for successful teeth were defined. Kaplan–Meier estimator was used to determine the success rate. Possible risk factors were compared between successful and unsuccessful groups by a Cox regression analysis to explore the potential predictors of failure with a significant level at α = 0.05.

Results

The mean ± SD of success time without considering variants was 6.2 ± 0.6 years (95% CI 5.1–7.7). The mean survival rates ± SD at 1.0-, 2.0-, 3.0-, 5.0-, 7.0-, and 9.0-year intervals was 97.8 ± 2.2%, 92.2 ± 4.4%, 72.8 ± 7.9%, 68.2 ± 8.6%, 60.7 ± 10.5%, and 40.4 ± 13.6%, respectively. Failure cases in teeth with poor plaque control and step-shaped fracture margin were significantly more than those with good plaque control and knife-shaped fracture margin (HR = 7.237,p = 0.011; HR = 15.399,p = 0.006; respectively).

Conclusions

Fractured teeth treated with modified crown lengthening surgery are anticipated to have a high clinical success rate for 6.2 ± 0.6 years. Plaque control and fracture morphology appeared to be significantly associated with the success of the multidisciplinary treatment approach.
A DSM-5-based tool to monitor concurrent mood and premenstrual symptoms: the McMaster Premenstrual and Mood Symptom Scale (MAC-PMSS)
Authors: Frey Benicio N. , Allega Olivia R. , Eltayebani Maha , Syan Sabrina K. , Mendes-Ribeiro Jeronimo , Minuzzi Luciano
Published: March 2022

Background

Despite high co-morbidity between premenstrual dysphoric disorder and mood disorders, there is a gap of research-based tools to monitor concurrent premenstrual and mood symptoms. In this study, we developed a new DSM-5-based questionnaire to prospectively monitor concurrent premenstrual and mood symptoms.

Methods

Fifty-two females with bipolar or major depressive disorder, ages 16–45, were enrolled in the study. Participants completed two months of prospective symptom charting including the McMaster Premenstrual and Mood Symptom Scale (MAC-PMSS) and the Daily Record of Severity of Problems (DRSP). At the end of the prospective charting, participants also completed the Montgomery-Åsberg Depression Rating Scale (MADRS), Hamilton Depression Rating Scale (HDRS) and the Young Mania Rating Scale (YMRS). The MAC-PMSS was correlated with the DRSP, MADRS, HDRS and YMRS.

Results

All individual items of the MAC-PMSS correlated strongly with the individual DRSP scores (all p < 0.001). The mood section of the MAC-PMSS also significantly correlated with MADRS (r = 0.572; p < 0.01), HDRS (r = 0.555; p < 0.01) and YMRS scores (r = 0.456; p < 0.01).

Conclusions

The MAC-PMSS is a reliable to tool to measure concurrent mood and premenstrual symptoms in women with mood disorders.
Huaier attenuates the adverse effects of pyroptosis by regulating the methylation of rat mesangial cells: an in vitro study
Authors: Geng Wenjia , Tu Can , Chen Dahao , Lu Zhaoyu , Mao Wei , Zhu Hanyu
Published: March 2022

Background

Pyroptosis is a highly programmed inflammatory cell death process that represents an innate immune response. In this study, the occurrence of pyroptosis in rat mesangial cells (RMCs) and the effect of Huaier (Trametes robiniophia Murr) on this process were investigated.

Methods

RMCs were incubated with OX7 antibodies (0.5 μg/ml, 2.5 μg/ml, 10 μg/ml), normal rat serum (NRS) and Huaier (1 mg/ml, 5 mg/ml, 10 mg/ml). RMC morphology was observed under a light microscope and by immunofluorescence. Lactate dehydrogenase (LDH) release was assessed using the CytoTox 96 Non-Radioactive Cytotoxicity Assay Kit. Western blot assays were performed, and then the RMCs were incubated with the methylase DNMT3B and the demethylase 5-aza-2′-deoxycytidine.

Results

Morphological, LDH, immunofluorescence and western blot analyses showed that RMCs were lysed when stimulated with OX7 antibodies and NRS. RMC lysis released inflammatory cytokines (interleukin-18, interleukin-1β, monocyte chemoattractant protein-1 and intracellular adhesion molecule-1), and Huaier protected RMCs by controlling lysis and the levels of inflammatory cytokines. Lysis was mediated by pyroptosis due to the positive expression of GSDME. The methylase DNMT3B reduced the expression of GSDME induced by OX7 together with NRS. Furthermore, Huaier significantly suppressed the expression of GSDME, which was increased by 5-aza-2’-deoxycytidine.

Conclusions

Pyroptosis might occur in RMCs, and Huaier can protect RMCs by upregulating the methylation of a group of molecules.
Assessment of the effectiveness of BOPPPS-based hybrid teaching model in physiology education
Authors: Liu Xiao-Yu , Lu Chunmei , Zhu Hui , Wang Xiaoran , Jia Shuwei , Zhang Ying , Wen Haixia , Wang Yu-Feng
Published: March 2022

Background

Online teaching has become increasingly common in higher education of the post-pandemic era. While a traditional face-to-face lecture or offline teaching remains very important and necessary for students to learn the medical knowledge systematically, guided by the BOPPPS teaching model, combination of online and offline learning approaches has become an unavoidable trend for maximizing teaching efficiency. However, in physiological education, the effectiveness of combined online teaching and offline teaching models remains poorly assessed. The present study aims at providing an assessment to the hybrid teaching model.

Methods

The study was performed among undergraduate medical students of Class 2017 ~ 2019 in the Physiology course in Harbin Medical University during 2018-2020. Based on established offline teaching model with BOPPPS components in 2018, we incorporated online teaching contents into it to form a hybrid BOPPPS teaching model (HBOPPPS, in brief), preliminarily in 2019 and completely in 2020. HBOPPPS effectiveness was assessed through comparing the final examination scores of both objective (multi-choice and single answer questions) and subjective (short and long essays) questions between classes taught with different modalities.

Results

The final examination score of students in Class 2019 (83.9 ± 0.5) who were taught with the HBOPPPS was significantly higher than that in Class 2017 (81.1 ± 0.6) taught with offline BOPPPS and in Class 2018 (82.0 ± 0.5) taught with immature HBOPPPS. The difference mainly attributed to the increase in average subjective scores (41.6 ± 0.3 in Class 2019, 41.4 ± 0.3 in Class 2018, and 38.2 ± 0.4 in Class 2017). In the questionnaire about the HBOPPPS among students in Class 2019, 86.2% responded positively and 79.4% perceived improvement in their learning ability. In addition, 73.5% of the students appreciated the reproducibility of learning content and 54.2% valued the flexibility of HBOPPPS. Lastly, 61.7% of the students preferred the HBOPPPS relative to BOPPPS in future learning.

Conclusions

HBOPPPS is likely a more effective teaching model and useful for enhancing effectiveness of Physiology teaching. This is attributable to the reproducibility and flexibility as well as the increased learning initiatives.
Opioid treatment program safety measures during the COVID-19 pandemic: a statewide survey
Authors: Bandara Sachini , Maniates Hannah , Hulsey Eric , Smith Jennifer S. , DiDomenico Ellen , Stuart Elizabeth A. , Saloner Brendan , Krawczyk Noa
Published: March 2022

Background

Opioid treatment programs (OTPs) serve as daily essential services for people with opioid use disorder. This study seeks to identify modifications to operations and adoption of safety measures at Pennsylvania OTPs during the COVID-19 pandemic.

Methods

A 25-min online survey to clinical and administrative directors at all 103 state-licensed OTPs in Pennsylvania was fielded from September to November 2020. Survey domains included: 1) changes to services, client volume, hours and staffing during the COVID-19 pandemic 2) types of services modifications 3) safety protocols to reduce COVID-19 transmission 4) challenges to operations during the pandemic.

Results

Forty-seven directors responded, for a response rate of 45%. Almost all respondents reported making some service modification (96%, n = 43). Almost half (47%, n = 21) of respondents reported reductions in the number of clients served. OTPs were more likely to adopt safety protocols that did not require significant funding, such as limiting the number of people entering the site (100%, n = 44), posting COVID-safety information (100%, n = 44), enforcing social distancing (98%, n = 43), and increasing sanitation (100%, n = 44). Only 34% (n = 14) of OTPS provided N95 masks to most or all staff. Respondents reported that staff’s stress and negative mental health (86%, n = 38) and staff caregiving responsibilities (84%, n = 37) during the pandemic were challenges to maintaining OTP operations.

Conclusion

OTPs faced numerous challenges to operations and adoption of safety measures during the COVID-19 pandemic. Funding mechanisms and interventions to improve adoption of safety protocols, staff mental health as well as research on patient experiences and preferences can inform further OTP adaptation to the COVID-19 pandemic and future emergency planning.
APOE ε4, Alzheimer’s disease neuropathology and sleep disturbance, in individuals with and without dementia
Authors: Blackman Jonathan , Love Seth , Sinclair Lindsey , Cain Richard , Coulthard Elizabeth
Published: March 2022

Background

Apolipoprotein E epsilon 4 (APOE-ε4) carrier status is an established risk factor for Alzheimer’s disease (AD) dementia. It has also been linked with sleep disturbance in healthy older adults and increased insomnia risk. This association may be driven by the effect of APOE-ε4 on AD pathological change, itself associated with sleep abnormalities. To assess this relationship, we have evaluated post-mortem neuropathological findings in patients with and without cognitive impairment and AD pathology, who had extensive clinical assessment within 12 months of death.

Methods

This retrospective cohort study used UK Brain Banks Network data. Eligible subjects were aged over 50, with pre-mortem neuropsychiatry inventory scores of sleep disturbance (NPI-K), neurocognitive testing and functional cognitive status assessment (Clinical Dementia Rating scale). Neuropathological data included Thal phase, Braak stage and CERAD scores (measures of Aβ plaque distribution, tangle distribution and neuritic plaque density, respectively) combined to form the National Institute on Aging Alzheimer’s Association (NIA-AA) ABC score reflecting AD neuropathology. Participants with other significant intracerebral pathology or pathological features of non-AD dementia were excluded.

Multivariate linear regression was performed with NPIK Global Score (NPIK frequency score multiplied by severity score) as the dependent variable and APOE-ε4 heterozygosity or homozygosity as independent variables. Covariates included age, gender, APOE-ε2 status and ABC NPI measures reflecting depression and anxiety. Further models stratified by ABC score and functional cognitive status were also produced.

Results

Seven hundred twenty-eight records were identified. Two hundred two participants were included in the final analysis: mean (SD) age 84.0 (9.2) and MMSE 14.0 (11.8). Mean sleep disturbance scores were highest in ε4 homozygosity (n=11), 4.55 (5.4); intermediate in ε4 heterozygosity (n=95), 2.03 (4.0); and lowest in non-ε4 carriers (n=96), 1.36 (3.3). Within the full sample, controlling for pathological status, age, gender, depression, anxiety and CDR-SOB status, APOE-ε4 homozygosity was associated with sleep disturbance (β 2.53, p=0.034). APOE-ε4 heterozygosity was similarly associated in individuals without dementia (β 1.21, p=0.048).

Conclusion

These findings lend weight to the hypothesis that APOE-ε4 affects sleep by mechanisms independent of AD pathological change. Evaluation of those mechanisms would enhance understanding of sleep disturbance pathways and potentially provide treatment targets.
Analysis of lung cancer-related genetic changes in long-term and low-dose polyhexamethylene guanidine phosphate (PHMG-p) treated human pulmonary alveolar epithelial cells
Authors: Lee Hong , Jeong Sang Hoon , Lee Hyejin , Kim Cherry , Nam Yoon Jeong , Kang Ja Young , Song Myeong Ok , Choi Jin Young , Kim Jaeyoung , Park Eun-Kee , Baek Yong-Wook , Lee Ju-Han
Published: March 2022

Background

Lung injury elicited by respiratory exposure to humidifier disinfectants (HDs) is known as HD-associated lung injury (HDLI). Current elucidation of the molecular mechanisms related to HDLI is mostly restricted to fibrotic and inflammatory lung diseases. In our previous report, we found that lung tumors were caused by intratracheal instillation of polyhexamethylene guanidine phosphate (PHMG-p) in a rat model. However, the lung cancer-related genetic changes concomitant with the development of these lung tumors have not yet been fully defined. We aimed to discover the effect of long-term exposure of PHMG-p on normal human lung alveolar cells.

Methods

We investigated whether PHMG-p could increase distorted homeostasis of oncogenes and tumor-suppressor genes, with long-term and low-dose treatment, in human pulmonary alveolar epithelial cells (HPAEpiCs). Total RNA sequencing was performed with cells continuously treated with PHMG-p and harvested after 35 days.

Results

After PHMG-p treatment, genes with transcriptional expression changes of more than 2.0-fold or less than 0.5-fold were identified. Within 10 days of exposure, 2 protein-coding and 5 non-coding genes were selected, whereas in the group treated for 27–35 days, 24 protein-coding and 5 non-coding genes were identified. Furthermore, in the long-term treatment group, 11 of the 15 upregulated genes and 9 of the 14 downregulated genes were reported as oncogenes and tumor suppressor genes in lung cancer, respectively. We also found that 10 genes of the selected 24 protein-coding genes were clinically significant in lung adenocarcinoma patients.

Conclusions

Our findings demonstrate that long-term exposure of human pulmonary normal alveolar cells to low-dose PHMG-p caused genetic changes, mainly in lung cancer-associated genes, in a time-dependent manner.
Epigenetic downregulation of Socs2 contributes to mutant N-Ras-mediated hematopoietic dysregulation
Authors: Jin Xi , Ng Victor , Zhao Meiling , Liu Lu , Higashimoto Tomoyasu , Lee Zheng Hong , Chung Jooho , Chen Victor , Ney Gina , Kandarpa Malathi , Talpaz Moshe , Li Qing
Published: March 2022

RAS mutations occur in a broad spectrum of human hematopoietic malignancies. Activating Ras mutations in blood cells leads to hematopoietic malignancies in mice. In murine hematopoietic stem cells (HSCs), mutant N-RasG12D activates Stat5 to dysregulate stem cell function. However, the underlying mechanism remains elusive. In this study, we demonstrate that Stat5 activation induced by a hyperactive Nras mutant, G12D, is dependent on Jak2 activity. Jak2 is activated in Nras mutant HSCs and progenitors (HSPCs), and inhibiting Jak2 with ruxolitinib significantly decreases Stat5 activation and HSPC hyper-proliferation in vivo in NrasG12D mice. Activation of Jak2-Stat5 is associated with downregulation of Socs2, an inhibitory effector of Jak2/Stat5. Restoration of Socs2 blocks NrasG12D HSC reconstitution in bone marrow transplant recipients. SOCS2 downregulation is also observed in human acute myeloid leukemia (AML) cells that carry RAS mutations. RAS mutant AML cells exhibited suppression of the enhancer active marker H3K27ac at the SOCS2 locus. Finally, restoration of SOCS2 in RAS mutant AML cells mitigated leukemic growth. Thus, we discovered a novel signaling feedback loop whereby hyperactive Ras signaling activates Jak2/Stat5 via suppression of Socs2.
Governance of inter-organizational systems: a longitudinal case study of Rotterdam’s Port Community System
Authors: Chandra Dissa R. , Van Hillegersberg Jos
Published: March 2022

An increasing use of inter-organizational systems, as Port Community System (PCS), can be observed in port collaborations. As multiple organizations often rely on PCS, even for business-critical processes, proper governance of these systems is crucial. This study aims to explain the governance of inter-organizational port collaborations using a lifecycles paradigm. The governance is explored using three points of view – i.e. governance mechanisms, governance aspects, and governance models – and by describing the actors’ roles in collaborations. A case study in the port of Rotterdam is analyzed to explain how these actors affect the governance models through the mechanisms to govern the aspects in each lifecycle stage. The port collaboration in Rotterdam has gone through three full governance lifecycles and has entered the fourth iteration after the set-up of Portbase. During the last two cycles, the collaboration has maintained its Network Administrative Organization governance model. This case study analysis of Rotterdam’s port collaboration provides an example of how a systematic approach could help to discuss and communicate the governance of inter-organizational port collaboration systems and gives some lessons learned for other collaborations.
IT program management challenges: insights from programs that ran into difficulties
Authors: Teubner R. Alexander
Published: March 2022

The use of Information Technology (IT) to drive organizational change has gained momentum in both for-profit and not-for-profit organizations, and currently culminates in a vivid discussion on what many call “Digital Transformation”. It is not surprising then that practitioners seek guidance on how to manage such transformation. Professional bodies have addressed this need by issuing best practice standards for Program Management (PgM), but we know little about their value in managing programs in general and IT programs in particular. Academic research on IT PgM is still in its very infancy. Taking this as motivation, we have investigated the challenges that managers faced in five IT programs that encountered problems. Our analysis reveals a set of management issues and shortcomings including, among others, a lack of architectural overview, difficulties in dealing with scope changes, stakeholder interest, diverse business groups and cultures, as well as a lack of internal PgM competences and unclear management responsibilities. In this paper, based on our findings, we present a first checklist for managing IT programs across their life cycle. Though still tentative and not necessarily comprehensive, we were able to confirm that our checklist provides relevant guidance for managing IT programs in practice.
Governance, governmentality and project performance: the role of sovereignty
Authors: Müller Ralf
Published: March 2022

Considerable confusion prevails in the mutual positioning and relationship of concepts like management, leadership, governance and governmentality in projects. This article first develops a framework to distinguish these terms conceptually by use of Archer’s structure and human agency philosophy. This provides for clearer conceptualization and lesser redundancy in the use of terms. Then the interaction between governance and governmentality in the context of projects is assessed, using a contingency theory perspective. This addresses long-standing questions about the nature of the impact of governance and governmentality on each other and on project and organizational performance. The results show that higher levels of project sovereignty (as a measure of governance), are associated with lower levels of authoritarian, but higher levels of neo-liberal governmentality, as well as higher levels of project and organizational performance. The article continues with a discussion of the theoretical implications from different perspectives of causality, which provides for different approaches to improve project performance through deliberate fine-tuning of governance and governmentality.
Go for it: Where IS researchers aren’t researching
Authors: Saunders Carol , Rutkowski Anne-F.
Published: March 2022

This viewpoint article describes two research topics under-researched by Information Systems (IS) researchers: Robotics and IT addiction. These topics offer great potential for IS researchers in terms of business and societal impacts and it would behoove IS researchers to study them more fully. The aspects of the research topics that are related to IS are discussed and potential research areas and questions are suggested.
Megaproject complexity attributes and competences: lessons from IT and construction projects
Authors: Nyarirangwe Maxwell , Babatunde Oluwayomi K.
Published: March 2022

Megaprojects have been associated with persistent underperformance technically, financially, socially and environmentally. This underperformance has been attributed to the inherent complexity attributes and the gaps in the form of the mismatch in the project management competences and processes used by the project management teams to deal with the complexity attributes. This study seeks to investigate the performance implications of these complexity attributes to recommend suitable management competences for the successful delivery of megaprojects. This conceptual study used an integrative literature review to analyze and synthesize findings from existing scientific articles related to the complexity constructs based on a comparative assessment of Information Technology (IT) and construction megaprojects. The Complex Adaptive Systems (CAS) Theory was also used to highlight some of the factors that influence megaproject performance towards identifying suitable management processes and competences, which are required to deal with megaprojects complexity. The key findings include a nomenclature of the main complexity attributes, their implications on the performance of IT and construction megaprojects, and, lastly, the managementcompetences and processes that are required to deal with the complexity attributes for improved megaproject performance.
Child sexual abuse: A ten-billion-pound problem
Authors: Shaw Gabrielle
Published: March 2022

Do you have something to share, reflect on or provoke thinking around? Write to the editor: angelica.celinska@markallengroup.com
Scattering evaluation of an infinite PEC plane coated with an anisotropic medium under arbitrary source excitation
Authors: He Jing-Jing , He Si-Yuan , Zhu Guo-Qiang , Li Wan-Cong , Hua Meng-Bo , Chao Li
Published: March 2022

A spectral-domain asymptotic method based on the dyadic Green’s function is proposed to study the electromagnetic (EM) scattering of an infinite perfectly conducting (PEC) plane coated with a uniaxial electric anisotropic medium (UEAM) under arbitrary source excitation. We specifically analyze the ray propagation path of the incidence wave from the individual point source, which acts at the planar-layered medium structure to obtain the dyadic Green’s functions (DGFs) of different types of EM waves. These DGFs from the reflected, transmitted, and entire secondary scattered fields are employed to establish the relationship between the arbitrary excitation source and different types of scattered fields. According to the corresponding “source-field” connection between the excitation source and the scattered field components in the spectral domain, we can first derive the expression of the spectral-domain DGF matrix components and then use the saddle point method to obtain the spatial-domain dyadic Green’s function. Finally, we use the integral equation solution to construct the scattered field for an infinite PEC plane coated with the UEAM layer under arbitrary source excitation. The comparison of simulation results from the proposed method and the method of moments (MoM) method confirms the algorithm validation. So it is expected to be a valid algorithm for dealing with electrically large and complex targets coated with the UEAM layer under any source excitation in the future.
Real-time transmission of geometrically-shaped signals using a software-defined GPU-based optical receiver
Authors: van der Heide Sjoerd , Luis Ruben S. , Goossens Sebastiaan , Puttnam Benjamin J. , Rademacher Georg , Koonen Ton , Shinada Satoshi , Awaji Yoshinari , Alvarado Alex , Furukawa Hideaki , Okonkwo Chigo
Published: March 2022

A software-defined optical receiver is implemented on an off-the-shelf commercial graphics processing unit (GPU). The receiver provides real-time signal processing functionality to process 1 GBaud minimum phase (MP) 4-, 8-, 16-, 32-, 64-, 128-ary quadrature amplitude modulation (QAM) as well as geometrically shaped (GS) 8- and 128-QAM signals using Kramers-Kronig (KK) coherent detection. Experimental validation of this receiver over a 91 km field-deployed optical fiber link between two Tokyo locations is shown with detailed optical signal-to-noise ratio (OSNR) investigations. A net data rate of 5 Gbps using 64-QAM is demonstrated.
Integrated visible-light liquid-crystal-based phase modulators
Authors: Notaros Milica , Dyer Thomas , Raval Manan , Baiocco Christopher , Notaros Jelena , Watts Michael R.
Published: March 2022

In this work, an integrated liquid-crystal-based phase modulator operating at visible wavelengths was developed and experimentally demonstrated. A visible-light silicon-nitride-based 300-mm-wafer foundry platform and a liquid-crystal integration process were developed to leverage the birefringence of liquid crystal to actively tune the effective index of a section of silicon-nitride waveguide and induce a phase shift over its length. The device was experimentally shown to achieve a 41π phase shift within 4.8 Vpp for a 500-µm-long modulator, which means that a 2π phase shifter would need to be only 24.4 µm long. This device is a compact and low-power solution to the challenge of integrated phase modulation in silicon nitride and paves the way for future low-power small-form-factor integrated systems at visible wavelengths.
Adaptive feedback threshold based demodulation for mobile visible light communication and positioning integrated system
Authors: Jin Jianli , Lu Huimin , Wang Jianping , Huang Jiacheng , Feng Lifang , Chen Danyang , Chen Hongyao , Dong Tao , Su Yuwei , Wang Yufeng
Published: March 2022

In this paper, a novel adaptive feedback threshold (AFT) based demodulation for mobile visible light communication and positioning (VLCP) integrated system is proposed. The AFT can vary with the received signal without excessive fluctuation and support communication and positioning in mobile environment. For the VLCP integrated system with single light emitting diodes (LED) and multiple photodetectors (PDs), maximal ratio combining (MRC) and received optical power ratio (ROPR) based on the AFT is further achieved for communication and positioning with high performance, respectively. It is demonstrated that high credible communication and high accuracy positioning for the mobile integrated VLCP system can be realized by using the proposed AFT based demodulation. As a result, the implemented VLCP system with a moving speed of 1 m/s is evaluated experimentally. Average positioning error of 3.43 cm with 800 mA current and the bit error ratio (BER) with different currents are also obtained.
Electro-optic frequency comb based IPDA lidar: assessment of speckle issues
Authors: Patiño William , Cézard Nicolas
Published: March 2022

We present a theoretical, numerical and experimental assessment of the impact of speckle on a dual electro-optic frequency comb (EOFC) based system for integrated path differential absorption (IPDA) measurements. The principle of gas concentration measurements in a dual EOFC configuration in the absence of speckle is first briefly reviewed and experimentally illustrated using a C2H2 gas cell. A numerical simulation of the system performance in the presence of speckle is then outlined. The speckle-related error in the concentration estimate is found to be an increasing function of the product between the roughness of the backscattering surface and the EOFC line-spacing. As this product increases, the speckle-induced power fluctuations in the comb lines are no longer correlated to each other. To confirm this, concentration measurements are conducted using backscattered light from two different surfaces. Experiment results are in very good agreement with numerical simulations. Though detrimental for IPDA measurements, it is finally shown that decorrelation of speckle noise can be advantageously exploited for surface characterization in a dual EOFC configuration.
Meta-learning-enabled accurate OSNR monitoring of directly detected QAM signals with one-shot training
Authors: Cheng Yijun , Yang Zheng , Yan Zhijun , Liu Deming , Fu Songnian , Qin Yuwen
Published: March 2022

We experimentally demonstrate meta-learning-enabled accurate optical signal-to-noise ratio (OSNR) monitoring of directly detected 16QAM signals with extremely few training data. When one-shot training, where one amplitude histogram (AH) for each OSNR value includes only 2000 data samples, is implemented for a 16QAM signal within a variable OSNR range of 15–24 dB, the experimental root mean squared error (RMSE) of the retraining technique is 1.53 dB. For transfer learning from the 16QAM simulation to the experimentally generated AH, the RMSE can be reduced to 1.11 dB. In comparison with both the retraining and transfer learning techniques, the RMSE of meta-learning-enabled OSNR monitoring can be further reduced by 42.8% and 22.3%, respectively. In order to reach the optimal accuracy with an RMSE of 0.66 dB, the meta-learning technique requires only 15 AHs for each OSNR value to be monitored, while the retraining and the transfer learning techniques need 20 and 25 AHs, respectively.
Direct conversion of Greenberger–Horne–Zeilinger state to Knill–Laflamme–Milburn state in decoherence-free subspace
Authors: Chen Lei , Xiu Xiao-Ming , Dong Li , Liu Nan-Nan , Shen Cai-Peng , Zhang Shou , Chen Shu , Su Shi-Lei
Published: March 2022

Several schemes are proposed to realize the conversion of photonic polarized-entangled Greenberger–Horne–Zeilinger state to Knill–Laflamme–Milburn state in decoherence-free subspace (DFS) via weak cross-Kerr nonlinearity and X-quadrature homodyne measurement with high fidelity. DFS is introduced to decrease the decoherence effect caused by the coupling between the system and the environment. Optimizations to improve the success rate and utilization of residual states are further investigated. This study indicates important applications for quantum information processing in the future.