bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of cycloheximide on the interpretation of ribosome profiling experiments in Schizosaccharomyces pombe
Authors: Duncan Caia D. S. , Mata Juan
Published: August 2017

Stress conditions lead to global and gene-specific changes in RNA translation. Ribosome profiling experiments have identified genome-wide alterations in the distribution of ribosomes along mRNAs. However, it is contentious whether these changes reflect real responses, or whether they are artefacts caused by the use of inhibitors of translation (notably cycloheximide). To address this issue we performed ribosome profiling with the fission yeast Schizosaccharomyces pombe under conditions of exponential growth (unstressed) and nitrogen starvation (nutritional stress), and both in the presence and absence of cycloheximide. We examined several aspects of the translational response, including density of ribosomal footprints on coding sequences, 5′ leader ribosomal densities, distribution of ribosomes along coding sequences, and ribosome codon occupancies. Cycloheximide had minor effects on overall ribosome density, which affected mostly mRNAs encoding ribosomal proteins. Nitrogen starvation caused an accumulation of ribosomes on 5′ leaders in both cycloheximide-treated and untreated cells. By contrast, stress-induced ribosome accumulation on the 5′ side of coding sequences was cycloheximide-dependent. Finally, codon occupancy showed strong positive correlations in cycloheximide-treated and untreated cells. Our results demonstrate that cycloheximide does influence some of the results of ribosome profiling experiments, although it is not clear if this effect is always artefactual.
Role of floral organ identity genes in the development of unisexual flowers of Quercus suber L.
Authors: Sobral Rómulo , Costa M. Manuela R.
Published: August 2017

Monoecious species provide an excellent system to study the specific determinants that underlie male and female flower development.Quercus suberis a monoecious species with unisexual flowers at inception. Despite the overall importance of this and other tree species with a similar reproductive habit, little is known regarding the mechanisms involved in the development of their male and female flowers. Here, we have characterised members of the ABCDE MADS-box gene family ofQ. suber. The temporal expression of these genes was found to be sex-biased. The B-class genes, in particular, are predominantly, or exclusively (in the case ofQsPISTILLATA), expressed in the male flowers. Functional analysis in Arabidopsis suggests that the B-class genes have their function conserved. The identification of sex-biased gene expression plus the identification of unusual protein-protein interactions suggest that the floral organ identity ofQ. subermay be under control of specific changes in the dynamics of the ABCDE model. This study constitutes a major step towards the characterisation of the mechanisms involved in reproductive organ identity in a monoecious tree with a potential contribution towards the knowledge of conserved developmental mechanisms in other species with a similar sex habit.
Supported Cu0 nanoparticles catalyst for controlled radical polymerization reaction and block-copolymer synthesis
Authors: Diacon Aurel , Rusen Edina , Mocanu Alexandra , Nistor Leona Cristina
Published: August 2017

The synthesis of Cu0 nanoparticles on different supports and their activity in controlled living radical polymerization processes is presented. The type of support influences the final size of the copper nanoparticles as well as their adhesion to the support. These aspects have a direct influence on the characteristics of the polymers obtained. The best results were obtained for SiO2 particles, which afforded a good molecular weight distribution (Mw/Mn = 1.25). The activity, recovery and recycling of the catalyst was explored for ultrafast polymerization reaction of butyl acrylate. Further, the terminal bromine reactivity was used for the synthesis of a block poly(n butyl acrylate-block-styrene). The influence of ligand type on the control of the reaction was studied. Also, a straightforward polymerization procedure without any ligand afforded a polydispersity value of 1.38.
In-situ visualization of multiple filament competition dynamic during nonlinear propagation of femtosecond laser
Authors: Qi Pengfei , Lin Lie , Su Qiang , Zhang Nan , Sun Lu , Liu Weiwei
Published: August 2017

In this work, multiple filamentation competition of femtosecond pulse in methanol is studied both experimentally and numerically. The visualization of multiple filamentation competition has been realized in the experiment performing the three-photon fluorescence of Coumarin 440. The random changes of multiple filamentation stemmed from the jitter of the peak laser intensity ratio of initial hot spots are first observed directly and visually, which can be well explained by a simplified (2D+1)-dimensional model.
Humans and climate change drove the Holocene decline of the brown bear
Authors: Albrecht Jörg , Bartoń Kamil A. , Selva Nuria , Sommer Robert S. , Swenson Jon E. , Bischof Richard
Published: August 2017

The current debate about megafaunal extinctions during the Quaternary focuses on the extent to which they were driven by humans, climate change, or both. These two factors may have interacted in a complex and unexpected manner, leaving the exact pathways to prehistoric extinctions unresolved. Here we quantify, with unprecedented detail, the contribution of humans and climate change to the Holocene decline of the largest living terrestrial carnivore, the brown bear (Ursus arctos), on a continental scale. We inform a spatially explicit metapopulation model for the species by combining life-history data and an extensive archaeofaunal record from excavations across Europe with reconstructed climate and land-use data reaching back 12,000 years. The model reveals that, despite the broad climatic niche of the brown bear, increasing winter temperatures contributed substantially to its Holocene decline — both directly by reducing the species’ reproductive rate and indirectly by facilitating human land use. The first local extinctions occurred during the Mid-Holocene warming period, but the rise of the Roman Empire 2,000 years ago marked the onset of large-scale extinctions, followed by increasingly rapid range loss and fragmentation. These findings strongly support the hypothesis that complex interactions between climate and humans may have accelerated megafaunal extinctions.
Development of a luciferase-based biosensor to assess enterovirus 71 3C protease activity in living cells
Authors: Zhang Yuan , Ke Xianliang , Zheng Caishang , Liu Yan , Xie Li , Zheng Zhenhua , Wang Hanzhong
Published: August 2017

Enterovirus 71 (EV71) is a major pathogen of hand, foot, and mouth disease (HFMD). To date, no antiviral drug has been approved to treat EV71 infection. Due to the essential role that EV71 3 C protease (3Cpro) plays in the viral life cycle, it is generally considered as a highly appealing target for antiviral drug development. In this study, we present a transgene-encoded biosensor that can accurately, sensitively and quantitatively report the proteolytic activity of EV71 3Cpro. This biosensor is based on the catalyzed activity of a pro–interleukin (IL)-1β-enterovirus 3Cprocleavage site-GaussiaLuciferase (GLuc) fusion protein that we named i-3CS-GLuc. GLuc enzyme is inactive in the fusion protein because of aggregation caused by pro–IL-1β. However, the 3Cproof EV71 and other enteroviruses, such as coxsackievirus A9 (CVA9), coxsackievirus B3 (CVB3), and poliovirus can recognize and process the canonical enterovirus 3Cprocleavage site between pro–IL-1β and GLuc, thereby releasing and activating GLuc and resulting in increased luciferase activity. The high sensitivity, ease of use, and applicability as a transgene in cell-based assays of i-3CS-GLuc biosensor make it a powerful tool for studying viral protease proteolytic events in living cells and for achieving high-throughput screening of antiviral agents.
Expression and anti-inflammatory role of activin receptor-interacting protein 2 in lipopolysaccharide-activated macrophages
Authors: Wu Qian , Qi Yan , Wu Na , Ma Chunhui , Feng Wenfang , Cui Xueling , Liu Zhonghui
Published: August 2017

The bacterial endotoxin lipopolysaccharide (LPS), a key pathogenic stimulator, can induce the activation of macrophages. Activin receptor-interacting protein 2 (ARIP2), an intracellular signaling protein, has a wide histological distribution, however, whether ARIP2 is involved in regulation of activation of macrophages was not well characterized. Here, by immunocytochemical staining, we found that ARIP2 protein existed in monocyte-macrophage cell line RAW264.7 cells and peritoneal macrophages of mouse, and ARIP2 expression in RAW264.7 cells was up-regulated by LPS. Furthermore, the results revealed that ARIP2 overexpression in the LPS-activated RAW264.7 cells inhibited the productions of IL-1β and TNFα, phagocytic activities and CD14 expression, whereas did not alter expressions of MyD88, TLR2 and TLR4. Additionally, in vivo ARIP2 overexpression also reduced the productions of IL-1β and TNFα from the LPS-stimulated peritoneal macrophages of mouse. These data suggest that ARIP2 may play an anti-inflammatory role in macrophages via inhibiting CD14 expression.
Breast Cancer Spheroids Reveal a Differential Cancer Stem Cell Response to Chemotherapeutic Treatment
Authors: Reynolds Daniel S. , Tevis Kristie M. , Blessing William A. , Colson Yolonda L. , Zaman Muhammad H. , Grinstaff Mark W.
Published: August 2017

An abnormal multicellular architecture is a defining characteristic of breast cancer and, yet, most in vitro tumor models fail to recapitulate this architecture or accurately predict in vivo cellular responses to therapeutics. The efficacy of two front-line chemotherapeutic agents (paclitaxel and cisplatin) are described within three distinct in vitro models employing the triple-negative basal breast cancer cell line MDA-MB-231 and the luminal breast cancer cell line MCF7: a) a 3D collagen embedded multicellular spheroid tumor model, which reflects the architecture and cellular heterogeneity of tumors in vivo; b) a 3D collagen model with a single cell-type diffusely embedded; and c) a 2D monolayer. The MDA-MB-231 embedded spheroid tumor model exhibited the most robust response to chemotherapeutic treatment, and possessed the greatest cancer stem cell (CSC) content. CSC-related genes are elevated across all MDA-MB-231 in vitro models following paclitaxel treatment, indicating that paclitaxel enrichment of chemoresistant CSCs is less dependent on microenvironmental tumor structure, while cisplatin showed a more context-dependent response. In the MCF7 cell models a context-dependent response is observed with paclitaxel treatment increasing the CSC related genes in the 2D monolayer and 3D diffuse models while cisplatin treatment afforded an increase in ALDH1A3 expression in all three models.
Effect of amino acid mutations on the conformational dynamics of amyloidogenic immunoglobulin light-chains: A combined NMR and in silico study
Authors: Mukherjee Sujoy , Pondaven Simon P. , Hand Kieran , Madine Jillian , Jaroniec Christopher P.
Published: August 2017

The conformational dynamics of a pathogenic κ4 human immunoglobulin light-chain variable domain, SMA, associated with AL amyloidosis, were investigated by 15N relaxation dispersion NMR spectroscopy. Compared to a homologous light-chain, LEN, which differs from SMA at eight positions but is non-amyloidogenic in vivo, we find that multiple residues in SMA clustered around the N-terminus and CDR loops experience considerable conformational exchange broadening caused by millisecond timescale protein motions, consistent with a destabilized dimer interface. To evaluate the contribution of each amino acid substitution to shaping the dynamic conformational landscape of SMA, NMR studies were performed for each SMA-like point mutant of LEN followed by in silico analysis for a subset of these proteins. These studies show that a combination of only three mutations located within or directly adjacent to CDR3 loop at the dimer interface, which remarkably include both destabilizing (Q89H and Y96Q) and stabilizing (T94H) mutations, largely accounts for the differences in conformational flexibility between LEN and SMA. Collectively, our studies indicate that a correct combination of stabilizing and destabilizing mutations is key for immunoglobulin light-chains populating unfolded intermediates that result in amyloid formation, and underscore the complex nature of correlations between light-chain conformational flexibility, thermodynamic stability and amyloidogenicity.
Pharmacogenetics of methylphenidate in childhood attention-deficit/hyperactivity disorder: long-term effects
Authors: Gomez-Sanchez Clara I. , Carballo Juan J. , Riveiro-Alvarez Rosa , Soto-Insuga Victor , Rodrigo Maria , Mahillo-Fernandez Ignacio , Abad-Santos Francisco , Dal-Ré Rafael , Ayuso Carmen
Published: August 2017

Attention-deficit/hyperactivity disorder (ADHD) is a common neurodevelopmental disorder in which a significant proportion of patients do not respond to treatment. The objective of this study was to examine the role of genetic risk variants in the response to treatment with methylphenidate (MPH). The effectiveness of MPH was evaluated based on variations in the CGI-S and CGAS scales over a 12-month treatment period using linear mixed effects models. A total of 208 ADHD patients and 34 polymorphisms were included in the analysis. For both scales, the response was associated with time, extended-release MPH/both formulations, and previous MPH treatment. For the CGI-S scale, response was associated with SLC6A3 rs2550948, DRD4 promoter duplication, SNAP25 rs3746544, and ADGRL3 rs1868790. Interactions between the response over time and SLC6A3 and DRD2 were found in the CGI-S and CGAS scales, respectively. The proportion of the variance explained by the models was 18% for the CGI-S and 22% for the CGAS. In this long-term study, the effects of SLC6A3, DRD4, SNAP25, and ADGRL3 on response to treatment reflect those observed in previous studies. In addition, 2 previously unreported interactions with response to treatment over a 12-month period were found (SLC6A3 and DRD2).
MDM2 Contributes to High Glucose-Induced Glomerular Mesangial Cell Proliferation and Extracellular Matrix Accumulation via Notch1
Authors: Lei Chun-Tao , Tang Hui , Ye Chen , You Chao-Qun , Zhang Jiao , Zhang Chun-Yun , Xiong Wei , Su Hua , Zhang Chun
Published: August 2017

Murine double minute 2 (MDM2) is an E3-ubiquitin ligase critical for various biological functions. Previous data have revealed an indispensable role of MDM2 in kidney homeostasis. However, its role in glomerular mesangial cell (GMC) proliferation and extracellular matrix (ECM) accumulation during hyperglycemia condition remains unclear. In our present study, we found that MDM2 protein level was significantly upregulated in high glucose-treated GMCs, while knocking down MDM2 by siRNA could attenuate high glucose-induced ECM accumulation and GMCs proliferation. Unexpectedly, Nutlin-3a, a MDM2-p53 interaction blocker, had no benefit in protecting diabetic mice from renal impairment in vivo and in alleviating high glucose-induced ECM accumulation in vitro. Intriguingly, we found that Notch1 signaling activation was obviously attenuated by MDM2 depletion in GMCs with high glucose exposure. However, Numb, a substrate of MDM2 which suppresses Notch1 signaling, was found not to be involved in the MDM2 and Notch1 association. Moreover, our findings demonstrated that MDM2 interacted with Notch1 intracellular domain (NICD1) independent of Numb and regulated the ubiquitination status of NICD1. Collectively, our data propose a pivotal role of MDM2 in high glucose-induced GMC proliferation and ECM accumulation, via modulating the activation of Notch1 signaling pathway in an ubiquitination-dependent way.
Modulation of action potentials using PEDOT:PSS conducting polymer microwires
Authors: Thourson Scott B. , Payne Christine K.
Published: August 2017

We describe the use of PEDOT:PSS conducting polymer microwires to modulate action potentials in single cells. PEDOT:PSS conducting polymer microwires are electrochemically synthesized with diameters ranging from 860 nm to 4.5 μm and conductivities of ~30 S/cm. The length of the microwires is controlled by the spacing of the electrodes used for the electrochemical polymerization. We demonstrate the use of these microwires to control the action potentials of cardiomyocytes, showing that the cellular contractions match the frequency of the applied voltage. Membrane integrity assays confirm that the voltage delivered by the wires does not damage cells. We expect the conducting polymer microwires will be useful as minimally invasive devices to control the electrical properties of cells with high spatial precision.
Enhanced Low-temperature Electro-optical Kerr Effect of Stable Cubic Soft Superstructure Enabled by Fluorinated Polymer Stabilization
Authors: Li Xiao , Yang Wei-Qiang , Yuan Cong-Long , Liu Zhen , Zhou Kang , Wang Xiao-Qian , Shen Dong , Zheng Zhi-gang
Published: August 2017

An enhanced electro-optical Kerr effect of the stable self-organized cubic blue phase liquid crystal superstructure at a relatively low temperature down to −50 °C was achieved through a judiciously designed fluorinated polymer stabilization. The fluorinated sample exhibited not only a rather stable cubic structure, but the promoted electro-optical performances of low driving voltage, weak hysteresis and high contrast ratio at such a low-temperature, which were much distinct from the common non-fluorinated polymer stabilized blue phase liquid crystal without conspicuous low-temperature Kerr response behaviours. Kerr constant, which reflects the obviousness of Kerr effect, of the fluorinated sample at −50 °C indicated a spectacular enhancement of two orders of magnitude in contrast to the commonly material, thereby corroborating the high efficiency of polymer fluorination in enhancing low-temperature Kerr effect. Such an enhancement of Kerr effect was probably resulted from the decreasing of interfacial anchoring between liquid crystal and fluorinated polymer network. The fluorinated polymer stabilization not only ensures the stability of self-organized cubic structure of blue phase, but overcomes the challenge and bottleneck problem of low-temperature inapplicability of common blue phase liquid crystal and paves a brilliant and broad way for relevant materials to abundant perspective applications at low temperature.
Morphine-induced hyperalgesia involves mu opioid receptors and the metabolite morphine-3-glucuronide
Authors: Roeckel Laurie-Anne , Utard Valérie , Reiss David , Mouheiche Jinane , Maurin Hervé , Robé Anne , Audouard Emilie , Wood John N. , Goumon Yannick , Simonin Frédéric , Gaveriaux-Ruff Claire
Published: August 2017

Opiates are potent analgesics but their clinical use is limited by side effects including analgesic tolerance and opioid-induced hyperalgesia (OIH). The Opiates produce analgesia and other adverse effects through activation of the mu opioid receptor (MOR) encoded by the Oprm1 gene. However, MOR and morphine metabolism involvement in OIH have been little explored. Hence, we examined MOR contribution to OIH by comparing morphine-induced hyperalgesia in wild type (WT) and MOR knockout (KO) mice. We found that repeated morphine administration led to analgesic tolerance and hyperalgesia in WT mice but not in MOR KO mice. The absence of OIH in MOR KO mice was found in both sexes, in two KO global mutant lines, and for mechanical, heat and cold pain modalities. In addition, the morphine metabolite morphine-3beta-D-glucuronide (M3G) elicited hyperalgesia in WT but not in MOR KO animals, as well as in both MOR flox and MOR-Nav1.8 sensory neuron conditional KO mice. M3G displayed significant binding to MOR and G-protein activation when using membranes from MOR-transfected cells or WT mice but not from MOR KO mice. Collectively our results show that MOR is involved in hyperalgesia induced by chronic morphine and its metabolite M3G.
Targeting transferrin receptors at the blood-brain barrier improves the uptake of immunoliposomes and subsequent cargo transport into the brain parenchyma
Authors: Johnsen Kasper Bendix , Burkhart Annette , Melander Fredrik , Kempen Paul Joseph , Vejlebo Jonas Bruun , Siupka Piotr , Nielsen Morten Schallburg , Andresen Thomas Lars , Moos Torben
Published: August 2017

Drug delivery to the brain is hampered by the presence of the blood-brain barrier, which excludes most molecules from freely diffusing into the brain, and tightly regulates the active transport mechanisms that ensure sufficient delivery of nutrients to the brain parenchyma. Harnessing the possibility of delivering neuroactive drugs by way of receptors already present on the brain endothelium has been of interest for many years. The transferrin receptor is of special interest since its expression is limited to the endothelium of the brain as opposed to peripheral endothelium. Here, we investigate the possibility of delivering immunoliposomes and their encapsulated cargo to the brain via targeting of the transferrin receptor. We find that transferrin receptor-targeting increases the association between the immunoliposomes and primary endothelial cells in vitro, but that this does not correlate with increased cargo transcytosis. Furthermore, we show that the transferrin receptor-targeted immunoliposomes accumulate along the microvessels of the brains of rats, but find no evidence for transcytosis of the immunoliposome. Conversely, the increased accumulation correlated both with increased cargo uptake in the brain endothelium and subsequent cargo transport into the brain. These findings suggest that transferrin receptor-targeting is a relevant strategy of increasing drug exposure to the brain.
A strategy for the identification of patterns in the biosynthesis of nonribosomal peptides by Betaproteobacteria species
Authors: Baldim João Luiz , da Silva Bruna Lidiane , Chagas-Paula Daniela Aparecida , Lago João Henrique G. , Soares Marisi G.
Published: August 2017

Nonribosomal peptides have an important pharmacological role due to their extensive biological properties. The singularities in the biosynthesis of these natural products allowed the development of genome-mining strategies which associate them to their original biosynthetic gene clusters. Generally, these compounds present complex architectures that make their identification difficult. Based on these evidences, genomes from species of the class Betaproteobacteria were studied with the purpose of finding biosynthetic similarities ﻿among them﻿. These organisms were applied as templates due to their large number of biosynthetic gene clusters and the natural products isolated from them. The strategy for Rapid Identification of Nonribosomal Peptides Portions (RINPEP) proposed in this work was built by reorganizing the data obtained from antiSMASH and NCBI with a product-centered way. The verification steps of RINPEP comprehended the fragments of existent compounds and predictions obtained in silico with the purpose of finding common subunits expressed by different genomic sequences. The results of this strategy revealed patterns in a global overview of the biosynthesis of nonribosomal peptides by Betaproteobacteria.
Addressing the challenges of applying precision oncology
Authors: Shin Seung Ho , Bode Ann M. , Dong Zigang
Published: August 2017

Precision oncology is described as the matching of the most accurate and effective treatments with the individual cancer patient. Identification of important gene mutations, such as BRCA1/2 that drive carcinogenesis, helped pave the way for precision diagnosis in cancer. Oncoproteins and their signaling pathways have been extensively studied, leading to the development of target-based precision therapies against several types of cancers. Although many challenges exist that could hinder the success of precision oncology, cutting-edge tools for precision diagnosis and precision therapy will assist in overcoming many of these difficulties. Based on the continued rapid progression of genomic analysis, drug development, and clinical trial design, precision oncology will ultimately become the standard of care in cancer therapeutics.
FANTOM5 CAGE profiles of human and mouse reprocessed for GRCh38 and GRCm38 genome assemblies
Authors: Abugessaisa Imad , Noguchi Shuhei , Hasegawa Akira , Harshbarger Jayson , Kondo Atsushi , Lizio Marina , Severin Jessica , Carninci Piero , Kawaji Hideya , Kasukawa Takeya
Published: August 2017

The FANTOM5 consortium described the promoter-level expression atlas of human and mouse by using CAGE (Cap Analysis of Gene Expression) with single molecule sequencing. In the original publications, GRCh37/hg19 and NCBI37/mm9 assemblies were used as the reference genomes of human and mouse respectively; later, the Genome Reference Consortium released newer genome assemblies GRCh38/hg38 and GRCm38/mm10. To increase the utility of the atlas in forthcoming researches, we reprocessed the data to make them available on the recent genome assemblies. The data include observed frequencies of transcription starting sites (TSSs) based on the realignment of CAGE reads, and TSS peaks that are converted from those based on the previous reference. Annotations of the peak names were also updated based on the latest public databases. The reprocessed results enable us to examine frequencies of transcription initiations on the recent genome assemblies and to refer promoters with updated information across the genome assemblies consistently.
Erratum: Lentiviral vectors can be used for full-length dystrophin gene therapy
Authors: Counsell John R. , Asgarian Zeinab , Meng Jinhong , Ferrer Veronica , Vink Conrad A. , Howe Steven J. , Waddington Simon N. , Thrasher Adrian J. , Muntoni Francesco , Morgan Jennifer E. , Danos Olivier
Published: August 2017

Scientific Reports 7: Article number: 44775; published online: 17 March 2017; updated: 29 August 2017. This article was published twice in error during a change in production systems. The publisher apologizes to the authors and readers for the error. When citing this work, please refer to the original version.
Erratum: Daytime warming has stronger negative effects on soil nematodes than night-time warming
Authors: Yan Xiumin , Wang Kehong , Song Lihong , Wang Xuefeng , Wu Donghui
Published: August 2017

Scientific Reports 7: Article number: 44888; published online: 20 March 2017; updated: 29 August 2017. This article was published twice in error during a change in production systems. The publisher apologizes to the authors and readers for the error. When citing this work, please refer to the original version.
Correction: Corrigendum: The NAC-type transcription factor OsNAC2 regulates ABA-dependent genes and abiotic stress tolerance in rice
Authors: Shen Jiabin , Lv Bo , Luo Liqiong , He Jianmei , Mao Chanjuan , Xi Dandan , Ming Feng
Published: August 2017

Scientific Reports 7: Article number: 40641; published online: 11 January 2017; updated: 29 August 2017. This Article contains an error in Figure 5a, where the panel ‘After Drought’ is incorrect. The correct Figure 5 appears below as Figure 1.
Genome-wide expression and response to exposure-based psychological therapy for anxiety disorders
Authors: Roberts S , Wong C C Y , Breen G , Coleman J R I , De Jong S , Jöhren P , Keers R , Curtis C , Lee S H , Margraf J , Schneider S , Teismann T , Wannemüller A , Lester K J , Eley T C
Published: August 2017

Exposure-based psychological treatments for anxiety have high efficacy. However, a substantial proportion of patients do not respond to therapy. Research examining the potential biological underpinnings of therapy response is still in its infancy, and most studies have focussed on candidate genes. To our knowledge, this study represents the first investigation of genome-wide expression profiles with respect to treatment outcome. Participants (n=102) with panic disorder or specific phobia received exposure-based cognitive behavioural therapy. Treatment outcome was defined as percentage reduction from baseline in clinician-rated severity of their primary anxiety diagnosis at post treatment and 6 month follow-up. Gene expression was determined from whole blood samples at three time points using the Illumina HT-12v4 BeadChip microarray. Linear regression models tested the association between treatment outcome and changes in gene expression from pre-treatment to post treatment, and pre-treatment to follow-up. Network analysis was conducted using weighted gene co-expression network analysis, and change in the detected modules from pre-treatment to post treatment and follow-up was tested for association with treatment outcome. No changes in gene expression were significantly associated with treatment outcomes when correcting for multiple testing (q<0.05), although a small number of genes showed a suggestive association with treatment outcome (q<0.5,n=20). Network analysis showed no association between treatment outcome and change in gene expression for any module. We report suggestive evidence for the role of a small number of genes in treatment outcome. Although preliminary, these findings contribute to a growing body of research suggesting that response to psychological therapies may be associated with changes at a biological level.
Simultaneous determination of ginsenosides Rg1, Re, and Rb1 and notoginsenoside R1 by solid phase extraction followed by UHPLC-MS/MS and investigation of their concentrations in various kinds of cosmetics
Authors: Peng Ying , Wu Zhijiang , Huo Yingpeng , Chen Yanwu , Lu Fenghui , Peng Qi , Liang Yingying
Published: August 2017

A solid phase extraction combined with UHPLC-MS/MS method has been developed and validated for determination of GRg1, GRe, GRb1 and NR1 in cosmetic products.
Diesel particulate matter and polycyclic aromatic hydrocarbons in fire stations
Authors: Bott Raymond C. , Kirk Katherine M. , Logan Michael B. , Reid Damien A.
Published: August 2017

Diesel exhaust emissions have been measured throughout the living and working areas of fire stations in Queensland, Australia.
Apoptosis triggered by isoquercitrin in bladder cancer cells by activating the AMPK-activated protein kinase pathway
Authors: Wu Ping , Liu Siyuan , Su Jianyu , Chen Jianping , Li Lin , Zhang Runguang , Chen Tianfeng
Published: August 2017

Our findings provide comprehensive evidence that isoquercitrin (ISO) influenced T24 bladder cancer cell metabolism by activating the AMPK pathway as identified by combination with metabolomics and immunoblotting assay.
The composition of potentially bioactive triterpenoid glycosides in red raspberry is influenced by tissue, extraction procedure and genotype
Authors: McDougall Gordon J. , Allwood J. William , Pereira-Caro Gema , Brown Emma M. , Latimer Cheryl , Dobson Gary , Stewart Derek , Ternan Nigel G. , Lawther Roger , O'Connor Gloria , Rowland Ian , Crozier Alan , Gill Chris I. R.
Published: August 2017

The composition of potentially bioactive triterpenoids differs between different tissues and varieties of raspberries, but are readily available from juices and purees.
Probing charges on solid–liquid interfaces with the resistive-pulse technique
Authors: Qiu Yinghua , Siwy Zuzanna
Published: August 2017

Surface charge of particles can be probedviaan approach curve of individual particles to a pore.
Rare earth ions enhanced near infrared fluorescence of Ag2S quantum dots for the detection of fluoride ions in living cells
Authors: Ding Caiping , Cao Xuanyu , Zhang Cuiling , He Tangrong , Hua Nan , Xian Yuezhong
Published: August 2017

We discovered that the fluorescence of Ag2S QDs can be enhanced by rare earth ions through AIE and then developed method for F− detection based on the coordination of rare earth ions with F−.
Preventing sintering of nanoclusters on graphene by radical adsorption
Authors: Martínez-Galera A. J. , Schröder U. A. , Herbig C. , Arman M. A. , Knudsen J. , Michely T.
Published: August 2017

The thermal stability of monodisperse transition metals nanoparticles on graphene is improved by exposure to radicals.
Phenyl-doped graphitic carbon nitride: photoluminescence mechanism and latent fingerprint imaging
Authors: Song Zhiping , Li Zhihong , Lin Lihua , Zhang Yongfan , Lin Tianran , Chen Ling , Cai Zhuang , Lin Sen , Guo Liangqia , Fu Fengfu , Wang Xinchen
Published: August 2017

Green-photoluminescent phenyl-doped graphitic carbon nitride (PDCN) powders were prepared and applied for the visualization of latent fingerprints.
Tandem Wittig/Diels–Alder diversification of genetically encoded peptide libraries
Authors: Triana Vivian , Derda Ratmir
Published: August 2017

In this paper, we developed a tandem of two carbon–carbon bond-forming reactions to chemically diversify libraries of peptides displayed on a bacteriophage.
Iron-catalyzed intermolecular cycloaddition of diazo surrogates with hexahydro-1,3,5-triazines
Authors: Liu Pei , Zhu Chenghao , Xu Guangyang , Sun Jiangtao
Published: August 2017

An iron-catalyzed formal [4 + 1]-cycloaddition of diazo compounds with hexahydro-1,3,5-triazines has been achieved.
A post-polymerization functionalization strategy for the synthesis of sulfonyl (trifluoromethanesulfonyl)imide functionalized (co)polymers
Authors: Ho Hien The , Tintaru Aura , Rollet Marion , Gigmes Didier , Phan Trang N. T.
Published: August 2017

Synthesis of functional sulfonyl(trifluoromethanesulfonyl)imide (STFSI) derivatives and original (co)polymers containing the STFSI group.
Mechanosynthesized copper(i) complex based initiating systems for redox polymerization: towards upgraded oxidizing and reducing agents
Authors: Garra P. , Dumur F. , Al Mousawi A. , Graff B. , Gigmes D. , Morlet-Savary F. , Dietlin C. , Fouassier J. P. , Lalevée J.
Published: August 2017

Significant improvements of the recently proposed Cu(i)/VitC + water/dibenzoyl peroxide (BPO) system for the redox free radical polymerization of methacrylates in air are presented here.
Enantioselective construction of tricyclic pyrrolidine-fused benzo[b]thiophene 1,1-dioxide derivatives via copper(i)-catalyzed asymmetric 1,3-dipolar cycloaddition
Authors: Deng Hua , He Fu-Sheng , Li Cong-Shan , Yang Wu-Lin , Deng Wei-Ping
Published: August 2017

A facile Cu(i)-catalyzed asymmetric 1,3-dipolar cycloaddition is developed, affording chiral tricyclic pyrrolidine-fused benzo[b]thiophene derivatives in excellent diastereo- and enantioselectivities.
Metal-mediated C–O bond forming reactions in natural product synthesis
Authors: Evano Gwilherm , Wang Jianjun , Nitelet Antoine
Published: August 2017

Metal catalyzed reactions for the formation of C(sp2)–O bonds have had a dramatic impact in natural product synthesis. They have enabled the emergence of new bond disconnections, which notably resulted in remarkably efficient and short synthetic pathways. The use of these reactions for the formation of C–O bonds in natural product synthesis is overviewed in this critical review.
Theoretical investigation on the mechanism of the OH-initiated degradation process of reactive red 2 azo dye
Authors: Luo Cheng , Wang Hongjie , Dong Wenyi , Zhang Xianbing
Published: August 2017

The dual descriptor (Δf) data of azo form (a, RR2) and hydrazone form (b, HRR2) of RR2 dianion. For Δf> 0 (green), the site is favorable for nucleophilic attack, for Δf< 0 (blue), the site is favorable for electrophilic attack. Key bond lengths in Å.
Facile one-pot synthesis of 3D graphite–SiO2 composite foam for negative resistance devices
Authors: Wu Ye , Fu Hao , Roy Amitava , Song Pengfei , Lin Yingcheng , Kizilkaya Orhan , Xu Jian
Published: August 2017

Facile one-pot synthesis of graphite–SiO2 foam for negative resistance devices.
Endocytosis mechanism of a novel proteoglycan, extracted from Ganoderma lucidum, in HepG2 cells
Authors: Yang Zhou , Wu Fan , Yang Hongjie , Zhou Ping
Published: August 2017

A novel protein tyrosine phosphatase 1B (PTP1B) inhibitor,FYGL, extracted fromGanoderma lucidum, was first reported to have an efficient hypoglycemic effect and high safetyin vivoin our previous study.
Synthesis and characterization of epitope-imprinted polymers for purification of human hemoglobin
Authors: Bagán Héctor , Zhou Tongchang , Eriksson Nélida Leiva , Bülow Leif , Ye Lei
Published: August 2017

On-particle tryptic digestion reveals surface epitopes suitable for molecular imprinting and protein recognition.
Improving piezoelectric properties by controlling phase structure and crystal orientation
Authors: Liu Baihui , Liu Xing , Li Peng , Li Feng , Shen Bo , Zhai Jiwei
Published: August 2017

〈001〉-textured (1 −x)K0.48Na0.52Nb0.96Sb0.04O3-xBi0.50(K0.48Na0.52)0.50Zr0.50Hf0.50O3lead-free ceramics prepared by templated grain growth (TGG) method are reported in this study.
Magnetic solid phase extraction with octahedral structured Fe3O4@SiO2@polydimethylsiloxane magnetic nanoparticles as the sorbent for determining benzene, toluene, ethylbenzene and xylenes in water samples
Authors: Zhang Maosheng , Huang Guobin , Huang Jiarong , Zhong Ling , Chen Weilan
Published: August 2017

Novel octahedral structured Fe3O4@SiO2@polydimethylsiloxane magnetic nanoparticles (Fe3O4@SiO2@PDMS MNPs) have been successfully synthesized for the first time.
Chiisanoside, a triterpenoid saponin, exhibits anti-tumor activity by promoting apoptosis and inhibiting angiogenesis
Authors: Bian Xingbo , Zhao Yan , Guo Xue , Zhang Lianxue , Li Pingya , Fu Tianhua , Wang Weidong , Yin Yongxia , Chen Guilin , Liu Jinping
Published: August 2017

Chissanoside from Acanthopanax species exhibits anti-tumor activity by protecting liver function, regulating immunity, promoting apoptosis and inhibiting angiogenesis.
Effect of Nb doping in WO3/ZrO2 catalysts on gas phase dehydration of glycerol to form acrolein
Authors: Sung Ku-Hsiang , Cheng Soofin
Published: August 2017

Gas-phase dehydration of glycerol to produce acrolein was investigated over tungstated zirconia catalysts with and without niobium doping, in order to correlate the catalytic activity and life-stability with the acid/base properties of the catalysts.
Zinc bimetallics supported by a xanthene-bridged dinucleating ligand: synthesis, characterization, and lactide polymerization studies
Authors: Hollingsworth Thilini S. , Hollingsworth Ryan L. , Rosen Tomer , Groysman Stanislav
Published: August 2017

Di-zinc complexes of a new dinucleating xanthene-based bis(iminophenolate) ligand have been synthesized, and their coordination chemistry and lactide polymerization activity have been investigated.
Identification of new quorum sensing autoinducer binding partners in Pseudomonas aeruginosa using photoaffinity probes
Authors: Baker Y. R. , Hodgkinson J. T. , Florea B. I. , Alza E. , Galloway W. R. J. D. , Grimm L. , Geddis S. M. , Overkleeft H. S. , Welch M. , Spring D. R.
Published: August 2017

Design, synthesis and application of PQS and HHQ probes for investigating quinolone quorum sensing pathways using photoaffinity labeling.
Pressure-induced dramatic changes in organic–inorganic halide perovskites
Authors: Lü Xujie , Yang Wenge , Jia Quanxi , Xu Hongwu
Published: August 2017

We summarise cutting-edge discoveries and provide insights into the important theme of halide perovskites using pressure as a tuning tool.
A novel peptide stapling strategy enables the retention of ring-closing amino acid side chains for the Wnt/β-catenin signalling pathway
Authors: Wu Ye , Li Ye-Hua , Li Xiang , Zou Yan , Liao Hong-Li , Liu Lei , Chen Ye-Guang , Bierer Donald , Hu Hong-Gang
Published: August 2017

An alternative all-hydrocarbon stapling approach in which the amino acid side chains are retained at the stapled positions.
Defining the current scope and limitations of dual noncanonical amino acid mutagenesis in mammalian cells
Authors: Zheng Yunan , Addy Partha Sarathi , Mukherjee Raja , Chatterjee Abhishek
Published: August 2017

We systematically evaluate potential platforms for site-specifically incorporating two distinct noncanonical amino acids into proteins expressed in mammalian cells with optimal fidelity and efficiency – a technology that will have many enabling applications.
Alternating current electrothermal modulated moving contact line dynamics of immiscible binary fluids over patterned surfaces
Authors: Kunti Golak , Bhattacharya Anandaroop , Chakraborty Suman
Published: August 2017

In this paper, we report the results of our numerical study on incompressible flow of a binary system of two immiscible fluids in a parallel plate capillary using alternating current electrothermal kinetics as the actuation mechanism for flow.
Nanoconfinement greatly speeds up the nucleation and the annealing in single-DNA collapse
Authors: Dai Liang , Jones Jeremy J. , Klotz Alexander R. , Levy Stephen , Doyle Patrick S.
Published: August 2017

Manipulating and measuring single-molecule dynamics and reactions in nanofluidics is a rapidly growing field with broad applications in developing new biotechnologies, understanding nanoconfinement effects in vivo, and exploring new phenomena in confinement.
Achiral flexible liquid crystal trimers exhibiting gyroid-like surfaces in chiral conglomerate phases
Authors: Sasaki Haruna , Takanishi Yoichi , Yamamoto Jun , Yoshizawa Atsushi
Published: August 2017

An achiral flexible trimer exhibits a gyroid-like surface and a sponge-like structure in the bulk of the material.
Charge storage at the nanoscale: understanding the trends from the molecular scale perspective
Authors: Vatamanu Jenel , Borodin Oleg , Olguin Marco , Yushin Gleb , Bedrov Dmitry
Published: August 2017

Molecular modeling of electrolytes near charged electrode surfaces provides key insights into fundamental mechanisms of charge storage at nanoscale operative in electric double layer capacitors, dual ion batteries and pseudo-capacitors.
Effect of sulfur-containing additives on the formation of a solid-electrolyte interphase evaluated by in situ AFM and ex situ characterizations
Authors: Lin Lingpiao , Yang Kai , Tan Rui , Li Maofan , Fu Shaojie , Liu Tongchao , Chen Haibiao , Pan Feng
Published: August 2017

In situ AFM reveals the evolution of SEI during initial cycles under the effect of additive.
A nanoporous graphene analog for superfast heavy metal removal and continuous-flow visible-light photoredox catalysis
Authors: Xiao Ran , Tobin John M. , Zha Meiqin , Hou Yun-Long , He Jun , Vilela Filipe , Xu Zhengtao
Published: August 2017

A metal-free conjugated porous polymer offers remarkable capabilities crucial for green and sustainable technologies, including removing 99.9% of lead from water within minutes, catalyzing quantitatively the Knoevenagel reaction in water, donor–acceptor units boosting photocatalytic activity and implementation in a continuous flow reactor.
A novel mixture of lithium bis(oxalato)borate, gamma-butyrolactone and non-flammable hydrofluoroether as a safe electrolyte for advanced lithium ion batteries
Authors: Shi Pei , Fang Shaohua , Huang Jun , Luo Dong , Yang Li , Hirano Shin-ichi
Published: August 2017

A safe electrolyte supported graphite/LiCo1/3Mn1/3Ni1/3O2 full cell to achieve excellent electrochemical performances.
Towards environmentally stable solution-processed oxide thin-film transistors: a rare-metal-free oxide-based semiconductor/insulator heterostructure and chemically stable multi-stacking
Authors: Cho Sung Woon , Kim Da Eun , Kim Kyung Su , Jung Sung Hyun , Cho Hyung Koun
Published: August 2017

ZTO/Al2O3 heterostructure and chemically stable sol–gel multi-stacking method are proposed for practical solution-processed oxide TFTs.
Metabolomics for the early detection of cisplatin-induced nephrotoxicity
Authors: Ezaki Takeshi , Nishiumi Shin , Azuma Takeshi , Yoshida Masaru
Published: August 2017

Metabolomics is a useful tool for discovering biomarkers to predict the side effects of cancer therapy.
Tributylphosphate (TBP) and tris (2-butoxyethyl) phosphate (TBEP) induced apoptosis and cell cycle arrest in HepG2 cells
Authors: Ren Guofa , Hu Jingwen , Shang Yu , Zhong Yufang , Yu Zhiqiang , An Jing
Published: August 2017

The purpose of this study was to investigate the cytotoxic effects of tributylphosphate (TBP) and tris (2-butoxyethyl) phosphate (TBEP) and to explore the underlying molecular mechanism focusing on oxidative stress, apoptosis, and cell cycle arrest.
Multipolar Hardy inequalities on Riemannian manifolds
Authors: Faraci Francesca , Farkas Csaba , Kristály Alexandru
Published: August 2017

We prove multipolar Hardy inequalities on complete Riemannian manifolds, providing various curved counterparts of some Euclidean multipolar inequalities due to Cazacu and Zuazua [Improved multipolar Hardy inequalities, 2013]. We notice that our inequalities deeply depend on the curvature, providing (quantitative) information about the deflection from the flat case. By using these inequalities together with variational methods and group-theoretical arguments, we also establish non-existence, existence and multiplicity results for certain Schrödinger-type problems involving the Laplace-Beltrami operator and bipolar potentials on Cartan-Hadamard manifolds and on the open upper hemisphere, respectively.
An Enantioselective Synthesis of (5S,6R,11S,14R)-Acremodiol
Authors: Dey Papiya , Chatterjee Sucheta , Gamre Sunita , Chattopadhyay Subrata , Sharma Anubha
Published: August 2017

An expeditious synthesis of the (5S,6R,11S,14R)-isomer of acremodiol was developed via a convergent route. One of the required building blocks was synthesized earlier via two sequential lipase-catalyzed secondary carbinol acetylations. The other unit was derived from (R)-2,3-cyclohexylideneglyceraldehyde as a chiral template. The bismacrolide skeleton was constructed by an intermolecular esterification reaction under Mitsunobu conditions followed by a ring-closing metathesis­ of the resultant α,ω-dialkenoic ester.
Laryngozele
Authors: Nawka Tadeus
Published: August 2017

Aufgrund ihrer Größe können Laryngozelen Beschwerden in Form von Heiserkeit, Atemnot oder Schluckbehinderung verursachen. Wenn diese
Symptome auftreten, ist eine operative Entfernung indiziert.
Botulismus: Diagnostik und Therapie
Authors: Wendt Sebastian , Eder Ines , Wölfel Roman , Braun Peggy , Lippmann Norman , Rodloff Arne
Published: August 2017

Hintergrund Botulismus ist eine seltene, lebensbedrohliche, zeitkritische neuroparalytische Erkrankung, die häufig Gegenstand differenzialdiagnostischer Erwägungen ist. Bezüglich Diagnostik und Therapie herrscht aber viel Unsicherheit. Rasche Diagnostik, frühzeitige Antitoxingabe, konsequente Lebensmittelhygiene und die Sensibilisierung der Bevölkerung können dazu beitragen, Inzidenz, Morbidität und Mortalität zu senken.

Methoden Grundlage ist eine epidemiologische Datenabfrage (RKI, ECDC, CDC, WHO) und selektive Literaturrecherche zu Diagnostik- und Therapieempfehlungen (PubMed bis März 2017). Ferner wurden die deutsche Botulismus-S1-Leitlinie (2012) und eigene Erfahrungen in der Diagnostik berücksichtigt.

Ergebnisse Die Inzidenz der durch den ubiquitären Sporenbildner Clostridium botulinum (Hauptvertreter) verursachten Botulinum-Intoxikation beträgt < 0,01/100 000 EU-Einwohner. Der Nahrungsmittelbotulismus ist ein reines Intoxikationssyndrom (häufigste Form) durch unsachgemäß zubereitete bzw. falsch gelagerte Lebensmittel. Wund- und Säuglingsbotulismus sind „Toxiko-Infektionen“. Im Vordergrund der Botulismus-Symptomatik steht die Neuroparalyse mit „bulbärem“ Beginn sowie bei Progredienz eine schlaffe Tetraparese bis hin zur Atemlähmung. Säuglingsbotulismus präsentiert sich unspezifisch und wird symptomatisch behandelt; ein spezielles Human-Antitoxin ist aber über internationale Apotheken erhältlich. Bei Verdacht auf Nahrungsmittel- bzw. Wundbotulismus muss schnellstmöglich Antitoxin verabreicht werden, das auch noch 24 Stunden nach Symptombeginn wirksam sein kann. Adjuvante Maßnahmen sind ohne Evidenz.

Schlussfolgerungen Trotz typischer Symptomatik wird Botulismus oft zu spät diagnostiziert. Frühzeitige Antitoxingabe und Intensivtherapie sind für das Überleben entscheidend. Zur Beratung sollte ein Konsiliarlabor kontaktiert werden.