bluuebunny/crossref_metadata_2025 huggingface dataset:
Tác động của kinh nghiệm, năng lực chuyên môn đến khả năng phát hiện gian lận trên báo cáo tài chính với vai trò trung gian của chủ nghĩa hoài nghi nghề nghiệp - trường hợp các công ty kiểm toán Non-Big4 Việt Nam
Authors: Trần Thị Bích Hiền
Published: January 2025

Bài viết nghiên cứu tác động của kinh nghiệm và năng lực chuyên môn đến khả năng phát hiện gian lận báo cáo tài chính, thông qua vai trò trung gian của chủ nghĩa hoài nghi nghề nghiệp của kiểm toán viên độc lập tại các công ty kiểm toán không thuộc Big4 tại Việt Nam. Phương pháp nghiên cứu định lượng được áp dụng, sử dụng mô hình cấu trúc tuyến tính bình phương nhỏ nhất từng phần (PLS-SEM) với sự hỗ trợ của phần mềm SmartPLS. Dữ liệu được thu thập từ 179 kiểm toán viên có chứng chỉ hành nghề, làm việc tại các công ty kiểm toán độc lập ngoài Big4. Kết quả nghiên cứu chỉ ra rằng cả ba yếu tố đều có tác động trực tiếp tích cực đến khả năng phát hiện gian lận báo cáo tài chính. Đồng thời, kinh nghiệm và năng lực chuyên môn của kiểm toán viên cũng ảnh hưởng gián tiếp đến khả năng này thông qua biến trung gian là chủ nghĩa hoài nghi nghề nghiệp. Trên cơ sở đó, bài viết đề xuất một số khuyến nghị nhằm nâng cao hiệu quả phát hiện gian lận báo cáo tài chính của kiểm toán viên độc lập tại Việt Nam.
Efficacy and safety analysis of Itopride Vs Bisacodyl in Chronic Constipation Cases at tertiary care Hospital in Prakasam District: A Prospective, randomized study.
Authors: Zaheera Mohammed , Gokul Thulasi , Kambar Chandrakala , Choppara Jhansi
Published: January 2025

Introduction: Chronic functional constipation is a functional bowel disorder characterized by difficult, infrequent, and incomplete defecation, affecting 14% of the world population. The quality of life and health care system are impacted and represents a heavy economic burden. Rome IV criteria are used for the diagnosis of constipation. Aim and objectives: To compare the safety and efficacy of Itopride vs Bisacodyl in chronic constipation, to evaluate improvement in constipation symptoms using the Validated PAC-SYM questionnaire, to evaluate treatment satisfaction in participants using a 5-point Likert Scale, Bristol Stool Form Score for Stool form assessment. Materials and methods: This prospective, randomized study was conducted in Government Medical College, Ongole, for 13 months, with the study population of 150 patients of both genders and aged >18 years. The data analysis is done using the PAC-SYM Score, 5-point Likert Scale, and Bristol Stool Form Scale (BSFS). Results: Compared to the bisacodyl group, The mean spontaneous complete bowel movements and spontaneous bowel movements were significantly higher in the itopride group (p < 0.05) at the end of the study. The overall satisfaction levels of patients taking Itopride and Bisacodyl do not significantly differ over time. Both drugs appear to have similar satisfaction profiles from baseline to 12 weeks. The BSFS scores differ significantly between Itopride and Bisacodyl during different time intervals. The similar satisfaction profiles suggest that both drugs, Itopride and Bisacodyl can be effectively used to manage constipation, depending on the patient's specific needs and preferences.
First whole genome sequencing of Staphylococcus aureus isolates from Iraq: Insights into zoonotic relations and biofilm-related genes
Authors: Bukhalifa Mohammed , Tameemi Hassan
Published: January 2025

Background:
Staphylococcus aureus is a significant zoonotic pathogen capable of causing infections in both humans and animals. The bacterium's capacity to develop biofilms and resistance to many different antibiotics has raised significant concerns for public health. Furthermore, studies have demonstrated that horizontal gene transfer enables the transfer of deleterious features between strains found in humans and animals, consequently rendering treatment and control efforts more challenging.

Aim:
This study aimed to investigate the relationships between human and animal isolates and biofilm-associated genes in local S. aureus strains using whole genome sequencing technique.

Methods:
We examined 111 suspected cases of Staphylococcus aureus infection in humans and in animals and screened all S. aureus -positive isolates (11 isolates) for biofilm formation and antimicrobial profiles. Additionally, we sequenced and studied five S. aureus genomes isolated from humans, cows, sheep, cats, and dogs for significant biofilm-related genes and predicted their loci following annotation and deposition in the NCBI database.

Results:
The study showed that the isolates have genome sizes between 2.7 and 2.8 megabases, a GC content of 32.8–33.1%, and a coding sequence count between 2,718 to 2,838. The cow isolate (MHB) and cat isolate (MHF) exhibited substantial genomic similarities with human isolates of S. aureus (N315) and the type strain of S. aureus (DSM 20231). The genomes of the human isolate (MHH) and the dog isolate (MHC) were comparable to S. aureus (N315). The sheep isolate (MHO) showed lesser genomic similarity and was closely related to S. aureus subsp. anaerobius. The genomes were submitted to the NCBI database with the following accession numbers: MHB (GCA_040196135.1), MHH (GCA_040196155.1), MHO (GCA_040195495.1), MHF (GCA_040195555.1), and MHC (GCA_040195445.1). The isolates were categorized by PubMLST typing into MHC (ST-1156), MHB (ST-6), MHF (ST-6), and MHO (a unique ST). We identified the accession numbers, locations, and lengths of biofilm-associated genes and regulators within the studied genomes.

Conclusion:
The study is the first to conduct complete genome sequencing of Staphylococcus aureus in Iraq, allowing analysis of biofilm-associated genes in local isolates. It provides the first large-scale genomic investigation of genetic relationships among animal and human isolates in Iraq.
Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae
Authors: Alyamani Najiah
Published: January 2025

Background:
Mitochondrial genomes play a key role in molecular biology research by providing essential information about evolutionary links, population history, and genetic diversity.

Aim:
The aim of this investigation was to produce partial sequence of 12S rRNA and 16S rRNA genes, as well as complete sequence of tRNA-Val gene in some species of family Labridae.

Methods:
Five species of labrid fishes (Oxycheilinus digramma, Cheilio inermis, Epibulus insidiator, Coris aygula and Gomphosus caeruleus) belong to Family Labridae were collected from the Red Sea, thereafter, taken to a lab for morphological identification in accordance with Randall (1982). Using forward and reverse primers (Wang, 2000), genome DNA was amplified through polymerase chain reaction.

Results:
The tRNA-Val gene's entire sequence, the 12S rRNA gene's partial sequence, and the 16S rRNA gene's partial sequence were all submitted to GenBank/NCBI with accession numbers (PP962382.1 - PP962386.1). The sequences' outcomes showed that the average A+T values were higher than the C+G values.

Conclusion:
The partial sequences of 12S RNA and 16S RNA, and the whole sequence of the tRNA-Val gene, were arranged that, the 12S RNA and 16S RNA have been distinguished by the tRNA-Val gene.
Evaluation of the anti-diabetic and anti-inflammatory potentials of curcumin nanoparticle in diabetic rat induced by streptozotocin
Authors: Alrashdi Barakat , Askar Hussam , Germoush Mousa , Fouda Maged , Farid Ibrahim , Massoud Diaa , Alzwain Sarah , Gadelmawla Mohamed , Ashry Mahmoud
Published: January 2025

Background:
Natural materials are frequently good options for drug development, regardless of their source. It has been demonstrated that curcumin boosts antioxidant capacity and guards against diabetic disorders.

Aim:
The current study aimed to evaluate the possible anti-inflammatory and anti-diabetic effects of curcumin-NPs (Cur-NPs) in streptozotocin (STZ)-induced diabetic rats.

Methods:
Four groups of rats were randomly selected; (1) standard control group, (2) Cur-NPs group was given the regular food of rats along with 5 mg/kg of Cur-NPs daily, (3) Diabetic rats in the STZ group served as the positive control, and (4) Included in the STZ~Cur-NPs group were diabetic rats receiving Cur-NPs (5 mg/kg/day).

Results:
After receiving Cur-NPs treatment for six weeks, the levels of glucose, TNF-α, IL1β, IL-4, IL-6, IL-10, MDA, and NO in the diabetic animals were significantly reduced. Simultaneously, the levels of insulin, CAT, GPx, GSH, and SOD were significantly increased, approaching the levels of the corresponding healthy animals. Similarly, insulin secretion increased in the islet β-cells as shown by immunohistochemical analysis, indicating improved glycaemic control and eventual glucose commitment to glycolysis; its processes for scavenging free radicals and acting as an antioxidant may explain this behavior.

Conclusion:
As a result, our findings aid in the potential characterization and creation of novel therapeutic agents that prevent diabetes.
A novel diagnostic technique for diagnosis of Staphylococcus aureus subclinical mastitis using gold nanoparticle-based ELISA
Authors: Gad Walaa , Osman Salama , Razik Khaled , Soror Ashraf , Fouad Ehab
Published: January 2025

Background:
Bovine subclinical mastitis is a significant cause of economic losses in dairy farms, primarily due to its asymptomatic nature and difficulty in early detection. The enzyme-linked immunosorbent assay (ELISA) is a widely used diagnostic tool in bioscience, facilitating antigen detection through antibody binding.

Aim:
The present study designed a gold nanoparticle (AuNPs)-based ELISA, to enhance the sensitivity of conventional ELISA by improving the binding efficiency of capture antibodies to purified antigens. This modified ELISA could enable more accurate detection of bovine subclinical mastitis.

Methods:
A total of 200 milk samples from apparently healthy cows were screened for subclinical mastitis using the California Mastitis Test (CMT). Positive samples were then subjected to bacteriological culture, biochemical testing, and PCR targeting the Nuc2 gene for confirmation of S. aureus. The purified fraction antigen of all 78 confirmed S. aureus isolates was extracted using sepharose 4B affinity column chromatography, described by SDS-PAGE, and assessed for its sensitivity in S. aureus mastitis diagnosis compared with crude antigen and purified fraction antigen-conjugated gold nanoparticles within an indirect enzyme-linked immunosorbent assay (ELISA). Immunoglobulins (IGS) from positive cow serum were extracted and purified from all confirmed S. aureus. Gold nanoparticle-based indirect ELISA was used on 400 samples (200 milk and 200 serum) from the same cows.

Results:
Using CMT, out of 200 examined milk samples from apparently normal cows, 65% (130/200) were sub-clinically infected. Out of these 130 positive milk samples, 60% (78/130) were confirmed to be infected with S. aureus. Purified fraction antigen-conjugated gold nanoparticles achieved the highest sensitivity to ELISA at 97%, 94%, and 92% immediately before storage, 6 months at -20 o C, and 1 year at -20 o C, respectively. Gold nanoparticle-based indirect ELISA detected specific IgG antibodies in 97% (76/78) sera and milk samples related to confirmed S. aureus isolates.

Conclusion:
The utilization of purified fraction antigen with gold nanoparticles enhances the sensitivity of ELISA, increasing it from 83.3% to 97% (p < .01; CI: 99%). The current study establishes a valuable way for S. aureus mastitis diagnosis within the use of purified fraction antigen-conjugated gold nanoparticles instead of the classical way to improve the sensitivity and the specificity of ELISA.
The standardization and efficacy of fermented Crescentia cujete (L.) in combination with enrofloxacin against artificially induced pneumonic pasteurellosis in rat models
Authors: Prakoso Yos , Susilo Achmadi , Widyarini Sitarina
Published: January 2025

Background:
Pasteurella multocida is an opportunistic bacterium causes pneumonic pasteurellosis (PP). The common treatment against PP is using antibiotics in conjunction with nonsteroidal anti-inflammatory drugs (NSAIDs). This combination presents various complications, i.e., immune-depression. Hence, the alternative therapy to replace the effects of NSAIDs needs to be clarified. One of them is using fermented calabash (Crescentia cujete (L.)) (FCC).

Aim:
This study aimed to elucidate the efficacy of FCC in combination with enrofloxacin against artificially induced PP in rat models.

Methods:
The calabash was collected and fermented. Moreover, the product of FCC was standardised regarding its biochemical compounds using LC-MS/MS and HPLC. This study used 30 male Sprague Dawley rats, weight 251.52±2.65 grams, six months old. The rats were divided into six groups as follows: G1 (control); G2 (infected with Pasteurella multocida + untreated); G3 (infected + 20 mg/kg enrofloxacin); G4 (infected + 20 mg/kg enrofloxacin + 30 mg/kg ibuprofen); G5 (infected + 20 mg/kg enrofloxacin + 2.96 mg/kg FCC); and G6 (infected + 20 mg/kg enrofloxacin + 5.92 mg/kg FCC). The treatment was given once daily for seven days. On day eight, the rats were radiographed. The serum was collected and tested against C-reactive protein (CRP) and procalcitonin. The rats were euthanized and lung tissue was collected for histopathology and immunohistochemistry against CD4+, CD8+ and COX-2. The data was analyzed using SPSS.

Results:
This study indicated that FCC contains choline, phytonadione, alpha-tocopherol, and retinol. Moreover, using FCC as a combination therapy with enrofloxacin against PP in group G6 promotes a repair of radiology image compared to other treatments (p<0.05). Group G5 and G6 showed increase activity of BALT, immune expression of CD4+ and COX-2, and the level of CRP and procalcitonin within the lung tissue (p<0.05). Group G6 indicated the better effects in various parameters in this study. However, the FCC has not influenced the immune expression of CD8+ during PP (p>0.05).

Conclusion:
This study proved that FCC could be used in rat models as an alternative anti-inflammatory treatment in combination with enrofloxacin against PP. Further research is needed to explore other effects of FCC to support the current findings.
Selection of Lactobacillus strains from native chicken feces for the fermentation of purple onion (Allium cepa L.) as an antibiotic alternative against Salmonella spp. in chickens
Authors: Hai Phan , Phuong Hoang , Hung Pham , Na Tran , Lai Ngo , Khuong Nguyen , Liem Tran , Hoa Nguyen
Published: January 2025

Background:
The increasing prevalence of antibiotic resistance in poultry pathogens necessitates the development of sustainable alternatives to antibiotics. Probiotics, particularly Lactobacillus spp., have shown promise in combating bacterial infections in poultry. Purple onion extract (OE) possesses antibacterial properties and can potentially enhance the probiotic efficacy of Lactobacillus strains.

Aim:
This study aimed to develop a biological product based on Lactobacillus-fermented OE (LFOE) as a sustainable alternative to antibiotics for the control of Salmonella-induced diarrhea in poultry.

Methods:
Lactobacillus strains were isolated from native free-range chicken feces and screened for their antibacterial activity against S. pullorum NCTC10705 and S. typhimurium FC13827, as well as their survival rate in OE. Six promising strains were selected and further characterized for their ability to ferment OE and their co-aggregation ability against the pathogenic bacteria using scanning electron microscopy. 16S rRNA gene sequencing was performed for bacterial identification. The selected strain was used for fermentation in OE, and the resulting product was freeze-dried into a biological preparation. In vivo studies in chicks were conducted to assess the safety and intestinal persistence of LFOE.

Results:
From an initial pool of 68 Lactobacillus strains, six promising candidates (L. plantarum 1582, L. plantarum WCFS1, L. plantarum JDM1, L. acidophilus NCFM, L. agilis DSM 20509, and L. agilis La3) were selected based on their antibacterial activity and high survival rate in OE. Scanning electron microscopy confirmed the ability of these strains to ferment OE and co-aggregate with pathogenic bacteria. 16S rRNA gene sequencing confirmed their taxonomic identity as Lactobacillus. L. plantarum 1582, selected for its superior probiotic properties, was used to ferment OE (LFOE), which proved safe for chicks and demonstrated the strain's ability to survive temporarily in the intestine.

Conclusion:
This study successfully developed a biopreparation based on LFOE as a potential alternative to antibiotics for the control of Salmonella-induced diarrhea in poultry. However, regular re-supplementation is required to maintain probiotic efficacy due to the transient nature of intestinal colonization.
Rancang Bangun Sistem Jemuran Pakaian Otomatis Berbasis Arduino
Authors: Ikromullah Mifad , Hafizul Haq Izham , Satria Putra Andi , Purwahyudi Bambang , Watiasih Richa
Published: January 2025

Jemuran pakaian merupakan keperluan primer yang sangat dibutuhkan oleh setiap orang. Sulitnya memperkirakan kondisi cuaca menjadi masalah utama ketika menjemur pakaian sehingga diperlukan jemuran pakaian otomatis untuk menyelesaikan masalah tersebut. Jemuran otomatis dapat dirancang dengan memanfaatkan sensor cahaya dan sensor hujan kemudian dikontrol menggunakan Arduino Uno untuk dapat membantu memudahkan menjemur pakaian tanpa harus khawatir keadaan cuaca. Rancang bangun dari sistem jemuran otomatis berhasil dibuat dan dapat bekerja sesuai yang diharapkan sehingga dapat menggerakkan jemuran ketika sesuai dengan kondisi cuaca pada lingkungan tersebut.
Hubungan Antara Pengetahuan dan Sikap Remaja dengan Seks Pra Nikah
Authors: Chodijah Siti , Sari Bulan Purnama , Tedengki Rein , Rizal Rossy Ville , Ayub Agis Dimas , Palola Jelsdryn
Published: January 2025

Masa remaja diawali dengan masa pubertas, yaitu masa terjadinya perubahan perubahan fisik, meliputi penampilan fisik dan fungsi fisiologis. Perubahan tubuh juga akan disertai dengan perkembangan bertahap dari karakteristik seksual primer dan karakteristik seksual sekunder. Perkembangan dan perubahan fisik pada remaja akan menyebabkan perubahan perilaku seksual remaja secara keseluruhan. Seks pranikah merupakan perilaku seksual yang dilakukan tanpa melalui proses pernikahan yang resmi menurut hukum maupun menurut agama dan kepercayaan masing-masing individu. Penelitian ini adalah untuk menganalisis Hubungan antara pengetahuan dan sikap remaja dengan seks pranikah di SMA Negeri 3 Pulau Rimau. Desain penelitian ini menggunakan metode korelasi analitik yaitu peneliti mencoba untuk mencari hubungan antar variabel, dengan pendekatan cross sectional yaitu jenis penelitian yang bertujuan untuk mengetahui hubungan antar variabel dimana variabel independent dan variabel dependen diidentifikasi pada satu satuan waktu.Penelitian ini menunjukkan hasil berdasarkan hasil perhitungan dengan menggunakan uji spearman rho dan analisa SPSS 22 didapatkan nilai rs=-0,386 dan nilai p=0,002 dimana p,0,05 maka H1 diterima, artinya terdapat hubungan pengetahuan dengan perilaku seks pranikah pada remaja usia di SMA Negeri 3 Pulau Rimau memiliki hubungan yang positif dan korelasi sempurna.Berdasarkan hasil analisis maka disimpulkan terdapat hubungan antara pengetahuan remaja dengan perilaku seks pranikah di SMA Negeri 3 Pulau Rimau. Demikian juga didapatkan hubungan antara sikap remaja dengan perilaku seks pranikah di SMA Negeri 3 Pulau Rimau.
An Investigation into Students’ Perceptions of Using Padlet as a Discussion Board at Saigon University
Authors: Vu Thi Kim Chi , Nguyen Hoang Thanh Tam , Dang Vu Minh Thu
Published: January 2025

Padlet.com is a cutting-edge platform enabling learners to collaborate effortlessly online by posting their work on a digital wall. Integrating Padlet into language teaching is thought to enhance remote learning, collaboration, and access to diverse language learning resources, making the learning experience more accessible, flexible, and interactive. The study aims to explore the perceptions of Saigon University students regarding using Padlet as a discussion board in their learning process. Both quantitative and qualitative methods were used to gather data through questionnaires and reflections. According to the results, the students acknowledge that the integration of Padlet enables them to create their responses in different forms, identify spelling mistakes and grammatical errors, as well as give peer assessments and comments. Additionally, most students agree that Padlet creates an enjoyable environment for productive and collaborative practice, which encourages active participation and engagement in their language learning classes. On the other hand, they admit that the greatest limitation is the stability of the Internet connection during the learning practice using Padlet.
Opioid prescribing patterns following surgical interventions for benign prostatic hyperplasia
Authors: Gressler Laura E. , Sze Christina , Punyala Ananth , Martinez-Diaz Susana , Bhojani Naeem , Zorn Kevin C. , Elterman Dean , Bilal Chughtai
Published: January 2025

Introduction: This study aimed to analyze opioid prescribing behavior following surgical interventions for benign prostatic hyperplasia (BPH), focusing on differences in postoperative opioid prescribing practices between patients who undergo surgical procedures in operative room (OR) settings vs. non-operative room (non-OR) settings.
Methods: A retrospective cohort study was conducted using a 10% random sample of the IQVIA PharMetrics® Plus for Academics database, including men who underwent surgical interventions for BPH from 2015–2020. Propensity score analysis and inverse probability treatment weighting were employed to adjust for potential confounders. Primary outcomes included opioid receipt, cumulative days of opioid use, and morphine equivalent daily dose (MEDD).
Results: Among the included men (n=6022), those undergoing procedures in OR settings were more likely to receive opioid prescriptions postoperatively compared to those in non-OR settings (42.78% vs. 28.00%, p<0.001). While cumulative days and MEDD of opioid prescriptions did not significantly differ between groups, there was a statistically significant difference in the distribution of opioid receipt duration (p=0.0128). The adjusted model showed significantly higher odds of opioid prescription for men undergoing OR procedures (odds ratio 1.922, 95% confidence interval 1.690–2.185).
Conclusions: Men undergoing BPH surgeries in OR settings were more likely to receive opioid prescriptions postoperatively, suggesting potential overprescription. Despite similar cumulative opioid use, differences in prescription rates indicate a need for improved postoperative pain management strategies, possibly using non-opioid alternatives. Future research should focus on optimizing pain control, characterizing actual opioid consumption, and considering patient-specific factors in surgical decision-making.
COLABORAÇÃO DO CIRURGIÃO DENTISTA NO DIAGNÓSTICO DE LESÕES OROFACIAIS EM CRIANÇAS OU ADOLESCENTES SUSPEITAS DE MAUS TRATOS FÍSICOS
Authors: Almeida Graça Tereza Cristina , Guimarães Fernanda Garcia de Andrade , Aguiar Telma Regina da Silva , Souza Cristiane Salgado de
Published: January 2025

Objetivo: Através de uma revisão narrativa de literatura, analisar o papel do cirurgião dentista na redução da violência doméstica, colaborando para denúncia de suspeita ou constatação de abusos contra crianças ou adolescentes. Metodologia: Foram realizadas buscas nas bases de dados: Scielo, Pubmed e BVS, utilizando-se os descritores selecionados na plataforma Descritores em Ciências da Saúde (DeCS): Child abuse, Childhood violence, dental surgeon, Forensic dentistry e orofacial manifestations.  Trabalhos publicados entre 2004 e 2024 que ofereceram, dentro do tema proposto, os descritores citados no resumo e título; trabalhos em inglês, português, francês e espanhol, foram consideradas no critério de inclusão. Os critérios de exclusão foram:  revisões de literatura, teses, monografias e capítulos de livro. Resultados: Com base na anamnese, relatos sobre como ocorreu a injúria e respaldado no diagnóstico diferencial, o cirurgião dentista será capaz de diagnosticar, conduzir o atendimento necessário e notificar às autoridades competentes suspeita ou constatação de abusos contra crianças ou adolescentes. Conclusão: O Cirurgião Dentista, bem como todo profissional da área de saúde, deve estar atento para a possibilidade de se ver diante de casos de maus tratos a crianças ou adolescentes, e deve alem de realizar os procedimentos terapeuticos indicados,  denunciar os casos  às autoridades competentes.
ENDODONTIC TREATMENT OF DECIDUOUS LOWER MOLAR DUE TO AGENESIS OF THE PERMANENT TOOTH GERM
Authors: Travassos Rosana Maria Coelho , Martins William Wale Rodrigues , Santos Pedro Guimarães Sampaio Trajano dos , Almeida Gustavo Moreira de , Chaves Adriane Tenório Dourado , Lima Samuel Nogueira , Lopes Daniela Siqueira , Milagres Christiano Ferraz , Cardoso Maria do Socorro Orestes , Ribeiro Fabrício Victer , Ferreira Glauco dos Santos
Published: January 2025

The objective of this study was to describe the preparation of root canals with a rotary system in a lower molar (85). A 23-year-old female patient was referred to the office of an endodontic specialist for endodontic therapy of her primary lower molar (85).  During the clinical examination, he reported pain on vertical percussion and occluso-distal composite resin restoration. Radiographic examination revealed deep restoration close to the distal pulp horn and agenesis of tooth 46. The probable diagnosis was acute apical periodontitis. Exploration of the root canals is carried out with a #10 C-pilot file, based on the apparent length of the tooth. Electronic odontometry was performed with the apex locator (Iroot Apex). The preparation of the root canals was carried out with a Prodesign Logic #30.05 file in the three root canals. During the preparation of each canal, 2% Chlorhexidine gel was used to facilitate the action of the nickel-titanium instruments.  The filling of the root canals, carried out in the same session with the calibrated MX single cone technique (Tanari) associated with Bio-C Sealer cement (bioceramic cement). Clinical and radiographic follow-up, carried out two years after filling the canals, determined the success of endodontic therapy. It is concluded that the endodontic treatment of deciduous teeth must be carried out in accordance with the technical standards of endodontics, aiming at sanitization, modeling and hermetic filling, determining reabsorption of the bioceramic cement extravasated at the time of filling, which has been proven during 2 years of maintenance. completion of endodontic therapy.
Lifelink Medical Emergency Handling App
Authors:   Student in Computer Science and Engineering & Presidency University, Bengaluru, N Meghana
Published: January 2025

This paper presents LifeLink, a novel system designed to address the critical challenges of medical emergency response in India. The system integrates advanced technologies such as GPS tracking, AI-powered risk assessment, and telemedicine to streamline emergency response, reduce response times, and improve healthcare outcomes. LifeLink aims to enhance coordination among different entities involved in emergency response, improve access to timely medical care, and ultimately save lives.

Key Words: Medical Emergency, Telemedicine, AI, GPS Tracking, Mobile App, IoT
Downregulation of proinflammatory cytokines and dynamic expression of TGF-β1 molecules induced by anti-IL-17 mAb in murine schistosomiasis mansoni
Authors:   Department of Medical Parasitology, Faculty of Medicine (girls), Al-Azhar University, Egypt., El-Lessy Fatma
Published: January 2025

Hepato-intestinal schistosomiasis is characterized by severe pathological changes at advanced chronic stages, including granulomatous lesions and liver fibrosis. The objective of our research was to assess the dynamic expression of profibrotic molecules, the transforming growth factor beta 1 (TGF-β1), and proinflammatory cytokines immunomodulation induced by interleukin 17 (IL-17) neutralization in murine Schistosomiasis mansoni. The study included 56 specific pathogen-free male C57BL/6 mice, divided into 3 main groups: GI uninfected normal controls, GII S. mansoni infected with 70±5 cercariae/non-treated, GIII S. mansoni infected and treated with anti-IL-17 monoclonal antibody (mAb), GIV S. mansoni infected and isotype-matched IgG2a mAb was given as a challenge. Mice were sacrificed at 6, 8, and 10 weeks after infection, then their liver enzymes and cytokines assessed, histopathological and immunohistochemical tested. The present study demonstrated a statistically significant elevation in serum levels of IL-17 (p<0.01), TGF-β1 (p<0.01), IL-1β (p≤0.001), IL-4 (p≤0.003), IL-6 (p≤0.05), and liver enzymes (ALT: p≤0.001; AST: p≤0.002). Additionally, granulomatous lesions and TGF-β1 expression were significantly increased (p<0.001) in infected mice at 6, 8, and 10 weeks after infection. All showed significant reduction by neutralization with anti-IL-17 mAb. Finally, IL-17 exhibited potent profibrogenic activity, and anti-IL-17 mAb can be used to alleviate and counteract this impact in murine S. mansoni.
Pengaruh ASI Eksklusif, Berat Badan Lahir Rendah, dan Tinggi Badan Ibu terhadap Kejadian Stunting pada Anak di Bawah Usia 2 Tahun di Kabupaten Tapin, Indonesia
Authors: Riza Ade
Published: January 2025

Latar belakang: Stunting memengaruhi pertumbuhan linier antar generasi. Ibu yang pendek berawal dari masa anak-anak stunting dan akan melahirkan bayi dengan berat badan lahir rendah (BBLR). ASI eksklusif diketahui sebagai salah satu faktor untuk mencegah stunting. Penelitian ini dilakukan untuk melihat pengaruh pemberian ASI eksklusif, riwayat berat badan lahir rendah, dan tinggi ibu terhadap kejadian stunting anak di bawah dua tahun di Kabupaten Tapin. Metode: Cross-sectional dengan uji analisis bivariat menggunakan Chi-Square dengan odds ratio untuk menilai risikonya. Hasil: Didapatkan hasil kejadian stunting berhubungan dengan tinggi ibu (p=0,002, OR 8,325, 95%CI 2,117–32,741). Tidak didapatkan adanya hubungan ASI eksklusif dan BBLR terhadap kejadian stunting (p=0,751, OR 1,303, 95%CI 0,454-3,738 untuk ASI eksklusif dan p=0,201, OR 2,222, 95%CI 0,849–5,818 untuk BBLR). Simpulan: Tinggi badan ibu memengaruhi kejadian stunting pada anak di Kabupaten Tapin.
ON THE ISSUE OF APPROACHES TO ASSESSING THE EFFECTIVENESS OF STATE REGULATION OF VEGETABLE GROWING DEVELOPMENT
Authors: Agibalov Aleksandr , Masik Aleksandr
Published: January 2025

The article discusses current theoretical and methodological approaches to assessing the effectiveness of government regulation of development. The qualitative characteristics of each approach and the degree of reliability of their results are determined. It is reasonable that the protected soil industry is a promising area within the framework of the state task to increase the level of provision of the population with vegetable products in the Central Asian Republic. A methodologically appropriate approach to determining the effectiveness of state regulation of vegetable production development using the production function has been chosen and its author's view based on the Cobb-Douglas model has been compiled. It has been established that specialized vegetable-growing enterprises are the object of reliable efficiency assessment in the Central Asian Republic. The proposed approach has been successfully tested. Conclusions have been drawn that have allowed us to establish an empirical basis for determining the effectiveness of state support at different levels of economic activity activities for the strategy of state regulation of the development of protected soil vegetable growing.
INTERNATIONAL COMPETITIVENESS OF THE BIOTECHNOLOGY INDUSTRY IN FRANCE IN THE CONTEXT OF THE COUNTRY'S SCIENTIFIC AND TECHNOLOGICAL DEVELOPMENT STRATEGY
Authors: Zaharova Natal'ya , Sekacheva Alla
Published: January 2025

The article reveals the specifics of the legislative framework of France, which considers the development of innovations in the country. In addition to certain laws that directly or indirectly regulate the activities of companies in the field of innovation, the authors note the importance of the provisions of the French Scientific and Technological Development Strategy. In the named Strategy the main focus is made on supporting innovation through effective tools to stimulate the innovative development of the national economy. The object of research in the article is the biotechnology sector that is one of the most competitive sectors of the French economy and according to the UNCTAD classification, contains "advanced" and in some cases "breakthrough" technologies. The development of this industry in the Fifth Republic is organized in clusters, contributes to the reindustrialization of the country's territories, increases employment and reduces carbon dioxide emissions.
The authors of the article conducted a comparative analysis of participants and funded projects of biotechnological clusters in France in 2024, using the annual cluster reports. Thus, the authors revealed that cluster financing in France occurs at the federal, regional and local levels. Besides the state as the main investor of innovative projects, France apply such an instrument like a system of public-private partnerships, as well as attracting private capital in the form of venture investments.
The authors note that, despite its high international competitiveness, the biotechnology industry in France faces certain challenges: difficulties in increasing the level of commercialization of products related to the timing of clinical trials; fact of luring companies with promising projects to other countries; complexity in financing start-ups; dependence on raw materials in biobanks located outside Europe; weak presence in global value chains. The authors propose specific measures to maintain and increase the level of international competitiveness in the field of healthcare biotechnology for the EU in general and France in particular.
METHODS AND CRITERIA FOR ASSESSING THE DEGREE OF ECONOMIC INTEGRATION USING THE EXAMPLE OF THE EUROPEAN UNION COUNTRIES
Authors: Zaharova Natal'ya , Sekacheva Alla
Published: January 2025

In this article the authors examined relevant experience of evaluating the effectiveness of economic integration using the example of the European Union as the most advanced integration association in the world nowadays. The article contains the analysis of the indices for integration measurement proposed by the supranational structures of the EU – the European Commission and the European Central Bank. The authors studied in detail two examples of approaches to assessing the effectiveness of integration within the EU by foreign researchers, which are based on an expanded theoretical and empirical base. The authors of the article highlight that besides the experience studied in the text, scientific publications also contain calculations of the old and new EU members integration level (Central and Eastern European countries), as well as integration and disintegration processes within the EU. Finally, the authors concluded that despite the methods adopted in the European Union for assessing the degree of its integration, this issue remains in the focus of attention of the European scientific community. Furthermore, the methodology for assessing the effectiveness of economic integration within the EU allows its leadership to timely adjust the development strategies of the integration association, which is strongly important in modern conditions. A question of integration is especially acute in the context of the systemic crisis in all EU countries, one of the main causes of which is the anti-Russian sanction policy.
EFFECT OF FAT GRAFTING ON IMPROVEMENT OF SCAR-AN INTERVENTIONAL STUDY
Authors: Abdul Sattar Sumaira , Faisal Akhlaq  , Waqas Sami  , Maryam Noor  , Farhan Zahid  , Unza Sheikh
Published: January 2025

Background: Scars are inevitable after effect of wound healing, a natural process that restores tissue integrity following injury, burns or surgery. They can be aesthetically displeasing and functionally impairing, depending on their location, size, and depth. Depending on the severity of the injury, scars can be hypertrophic, atrophic, or keloid. These variations can lead to pain, itching, contractures (limited movement), and psychological distress, impacting a patient's quality of life. Objective was to evaluate the efficacy of autologous fat grafting in improving the appearance and quality of scars, with a focus on patients presenting with facial scars due to burns, trauma, surgery or acne at a tertiary care hospital in Karachi, Pakistan. Methods: In this quasi-experimental study, 30 patients of age 18 years to 60 years, irrespective of gender having facial scar because of burn, trauma, surgery or acne were included in the study. A comprehensive scar assessment was performed using the Patient and Observer Scar Assessment Scale (POSAS) before treatment, and follow-up evaluations were conducted on the seventh day, at three months, and at six months post-fat grafting. Fat grafting was executed using a tumescent technique based on Klein's formula, with the aspirated fat injected into the subdermal plane of the scar. Data analysis was performed using SPSS version 23. Results: The patient cohort had a mean age of 26.77 years, with a predominance of female participants (73.3%). Trauma was the most common cause of scars (50%), with the forehead being the most frequent location (36.67%). Significant improvements in scar quality were observed, with both patient and observer POSAS scores showing notable reductions from baseline to 6 months (p<0.05). All patients (100%) demonstrated an improvement in scar appearance. Conclusion: Fat grafting presents a promising treatment for improving the appearance and quality of facial scars.
AMPHETAMINE-INDUCED PSYCHOSIS LEADING TO HOMICIDE, SUICIDAL ATTEMPTS, AND DISORIENTATION: A CASE REPORT
Authors: Hussain Azad Abrar , Shahzad Ali Khan  , Iram Bibi  , Ijaz Ali  , Pashma Wazir  , Shaaray Abrar Umar
Published: January 2025

Amphetamine abuse leads to severe psychiatric and cardiovascular complications–psychosis, hallucinations and aggressive behavior. A 34-year-old male of five years noted history of amphetamine abuse and presented with amphetamine psychosis characterized by hallucinations, violent ideations and a recent suicide attempt is described in this case report. Previously under the influence of amphetamines, two years prior, the patient committed homicide. Patient had periods of very severe disorientation and agitation, history of suicidal ideation and violent thoughts toward others. Toxicology screenings were done, all were consistent with amphetamine presence. Diagnostic studies included cardiac biomarker elevations and abnormal electrolyte levels. Management was immediate and involved sedation, antipsychotic therapy and cardiovascular support, followed by long term psychiatric care and substance abuse rehabilitation. Here, chronic amphetamine abuse is shown to have profound psychiatric and behavioral effects and to require integrated treatment strategies to meet the complex needs of such patients.
Analysis of Factors Influencing the Mandarin Proficiency of Ethnic Minority College Students
Authors: Xiaohua  , Changlin
Published: January 2025

This study focuses on determining the factors that influence Mandarin proficiency of ethnic minority college students of Hulunbuir University in border minority areas of China. We used questionnaire to collect multi-dimensional data including gender, living environment, family language environment, parents’ Mandarin proficiency, parents’ academic qualifications, regional differences and parents’ occupations. Descriptive statistics, cross-contingency and chi-square tests were used to analyze the data and reveal the key factors affecting Mandarin proficiency. The results show that girls’ Mandarin proficiency is generally better than that of boys (x² = 25, p < 0.01), and students from cities and flag counties perform better in Mandarin, compared to students from rural and pastoral areas (x² = 27, p < 0.05). Family language environment(x² = 38, p < 0.01), Mandarin proficiency (x²=37, p < 0.05), occupation and academic qualifications of parents and regional background all significantly affect students' Mandarin proficiency (x 2 = 40.8, p < 0.01). Based on these findings, this study recommends the following measures to improve Mandarin education: increase investment in Mandarin education in minority areas, optimize the language learning environment, encourage parents to improve their Mandarin proficiency, and carry out more Mandarin training and practice activities in schools, while develop personalized teaching strategies for students from different backgrounds.
Psychological consequences of the digital transformation of the translation industry: an exploratory study of technostress among Danish certified translators
Authors: Paulsen Christensen Tina , Bundgaard Kristine , Flanagan Marian
Published: January 2025

Based on survey data, the study explores the consequences of digital transformation for the well-being of Danish certified translators, focusing on the level of technostress related to translators’ adoption of CAT tools, neural machine translation tools or no tools, as well as to translators’ professional translation experience.
Influência da leflunomida nos níveis de ácido úrico sérico em pacientes com artrite reumatoide
Authors: Kahlow Bárbara Stadler , Tonet Camila , Vieira Maria Fernanda Correa , Neukamp Marcelo Budke , Skare Thelma Larocca
Published: January 2025

Introdução: Inflamação é componente comum das doenças autoimunes como a artrite reumatoide. Há crescentes evidências do papel dele sérico no seu processo inflamatório e possível influência na atividade da doença.
Objetivo: Correlacionar níveis séricos de ácido úrico à atividade da doença, aos fatores de pior prognóstico e medicamentos em uso em pacientes com essa artrite.
Método: Coletou-se dados relacionados à epidemiologia, sintomatologia, tratamentos farmacológicos e escores de doença de prontuários de 105 pacientes com artrite reumatoide, correlacionando-os com os níveis séricos de ácido úrico, como biomarcador.
Resultado: O valor mediano de ácido úrico sérico foi de 3,4 mg/dL e não houve significância entre ele e scores de atividade de doença. Ao associá-lo com hipertensão (p = 0,0014), diabete melito (p = 0,058), leflunomida (p = 0,0276) e inibidores de JAK (p = 0,02) observou-se significância. Contudo, demonstrou-se correlação significativa apenas entre menores taxas de ácido úrico sérico e o uso de leflunomida (p = 0,0192; t = -2,382).
Conclusão: A variação nos níveis séricos de ácido úrico não demonstrou impacto na atividade da doença. Os pacientes em uso de leflunomida apresentaram níveis reduzidos do biomarcador, reforçando seu efeito hipouricêmico.
Študija motivacije srednješolcev in prepričanj učiteljev o motivaciji
Authors: Čelofiga Jernej
Published: January 2025

Izvedena študija se osredotoča na problem učne motivacije med učenci srednjih šol in jo primerja s prepričanji učiteljev o tem, kaj motivira učence. Hkrati preučuje povezave med dejavniki učne motivacije in samopodobo glede izgovorjave ter učne motivacije. Kot del obsežnejše raziskave ta drobec izbrane teme ponuja vpogled v povezavo med motivacijo, učno motivacijo, samopodobo motivacije in prepričanji učiteljev o motivaciji učencev. Omenjeni motivacijski dejavniki so razdeljeni na zunanje in notranje dejavnike. Izkaže se, da so učitelji našteli enake ali podobne motivacijske dejavnike kot učenci, kar kaže na odsotnost vrzeli v dojemanju tega, kaj motivira. Učitelji natančno vedo, zakaj se učenci učijo. Edina odstopanja so v tem, da učitelji verjamejo, da je motivacija, ki je pridobljena z ocenami, bolj prisotna, kot v resnici je.
Bimbingan Teknis Penyusunan Laporan Pertanggungjawaban sebagai Optimalisasi Dana Corporate Social Responsibility untuk Pembangunan Berkelanjutan di Sidoarjo
Authors: Fitrianah Listin , Zahrotul Azizah  , Fitri Medya Ayunda , Hakim Luqman , Yafi Muhammad Mansur
Published: January 2025

The problem of river water pollution caused by waste and garbage disposal has reduced water quality, ecosystem carrying capacity, and natural resources, especially in Sidoarjo Regency. This study aims to ensure that Corporate Social Responsibility (CSR) funds are utilized by the principles of sustainable development so that they positively impact society and the environment. The methods used involve the planning and socialization stages, allocation of CSR funds, implementation of activities, technical guidance, monitoring and evaluation, and accountability reports. The results show that technical guidance provides an in-depth understanding of transparency, accountability, and sustainability in managing CSR funds, increasing villages' capacity to manage funds effectively. This community service contributes to the management of CSR funds that are more focused on supporting environmental conservation and the welfare of local communities.
Efektivitas Pelatihan Microsoft Office dan Canva dalam Meningkatkan Kemampuan Teknologi Digital
Authors: Putri Irsya Yuwana , Mustaqim M. , Azizah Siti Nur , Khalimah Siti Nur
Published: January 2025

Pelatihan teknologi digital sangat penting untuk menjembatani kesenjangan keterampilan peserta didik dalam menghadapi dunia kerja berbasis teknologi. Kegiatan pengabdian ini bertujuan meningkatkan kemampuan peserta dalam pembuatan dokumen, pengelolaan data, dan desain presentasi multimedia. Metode pelatihan meliputi penyampaian materi teoretis, praktik langsung, dan pendampingan intensif, dengan evaluasi yang menunjukkan peningkatan signifikan dalam keterampilan peserta. Program ini menawarkan pendekatan unik melalui integrasi Microsoft Office dan Canva, yang berkontribusi pada peningkatan kemampuan komunikasi visual peserta. Hasil pelatihan ini diharapkan dapat direplikasi di institusi pendidikan lain untuk mendukung kesiapan tenaga kerja yang kompetitif di era digital.
Digital technology training is essential to bridge the gap in students' skills in facing the technology-based world of work. This community service activity aims to improve participants' document creation, data management, and multimedia presentation design abilities. The training method includes delivering theoretical materials, hands-on practice, and intensive mentoring, with evaluations showing significant improvements in participants' skills. This program offers a unique approach by integrating Microsoft Office and Canva, which contributes to improving participants' visual communication skills. The results of this training are expected to be replicated in other educational institutions to support the readiness of a competitive workforce in the digital era.
Implementation of the Policy on the Use of the BKN e-Performance Application in the Southeast Minahasa Regency Government Environment
Authors: Mokosolang Merry Natalia , Tasik Femmy C M , Egeten Maxi M
Published: January 2025

The use of the e-Performance application will result in effectiveness and efficiency in ASN performance reporting. ASN employees no longer need to print their monthly performance results but can be directly entered into the application and can be directly assessed by the assessor without being limited by time, can be done anywhere and anytime. This study aims to determine what factors are the causes of the failure to implement the BKN e-Performance Application in the Southeast Minahasa Regency Government properly. Qualitative design was used in this study. The Human Resources Development and Personnel Agency as the research location. The focus of the study is based on aspects of communication, resources, attitudes of implementers and bureaucratic structure. Data analysis through the stages of data reduction, data presentation and preparation of conclusions and verification. The findings of the study were miscommunication in ASN in translating socialization materials, inadequate internet facilities, ASN who were still indifferent in reporting their monthly performance, and there were no standard binding rules regarding awards and sanctions regarding the use of the BKN e-Performance application for ASN.