bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of cohesive subgroups in a university hall of residence during the COVID-19 pandemic using a social network analysis approach
Authors: Marqués-Sánchez Pilar , Pinto-Carral Arrate , Fernández-Villa Tania , Vázquez-Casares Ana , Liébana-Presa Cristina , Benítez-Andrades José Alberto
Published: November 2021

The aims: (i) analyze connectivity between subgroups of university students, (ii) assess which bridges of relational contacts are essential for connecting or disconnecting subgroups and (iii) to explore the similarities between the attributes of the subgroup nodes in relation to the pandemic context. During the COVID-19 pandemic, young university students have experienced significant changes in their relationships, especially in the halls of residence. Previous research has shown the importance of relationship structure in contagion processes. However, there is a lack of studies in the university setting, where students live closely together. The case study methodology was applied to carry out a descriptive study. The participation consisted of 43 university students living in the same hall of residence. Social network analysis has been applied for data analysis. Factions and Girvan–Newman algorithms have been applied to detect the existing cohesive subgroups. The UCINET tool was used for the calculation of the SNA measure. A visualization of the global network will be carried out using Gephi software. After applying the Girvan–Newman and Factions, in both cases it was found that the best division into subgroups was the one that divided the network into 4 subgroups. There is high degree of cohesion within the subgroups and a low cohesion between them. The relationship between subgroup membership and gender was significant. The degree of COVID-19 infection is related to the degree of clustering between the students. College students form subgroups in their residence. Social network analysis facilitates an understanding of structural behavior during the pandemic. The study provides evidence on the importance of gender, race and the building where they live in creating network structures that favor, or not, contagion during a pandemic.
Fine-scale spatial segregation in a pelagic seabird driven by differential use of tidewater glacier fronts
Authors: Bertrand Philip , Bêty Joël , Yoccoz Nigel G. , Fortin Marie-Josée , Strøm Hallvard , Steen Harald , Kohler Jack , Harris Stephanie M. , Patrick Samantha C. , Chastel Olivier , Blévin P. , Hop Haakon , Moholdt Geir , Maton Joséphine , Descamps Sébastien
Published: November 2021

In colonially breeding marine predators, individual movements and colonial segregation are influenced by seascape characteristics. Tidewater glacier fronts are important features of the Arctic seascape and are often described as foraging hotspots. Albeit their documented importance for wildlife, little is known about their structuring effect on Arctic predator movements and space use. In this study, we tested the hypothesis that tidewater glacier fronts can influence marine bird foraging patterns and drive spatial segregation among adjacent colonies. We analysed movements of black-legged kittiwakes (Rissa tridactyla) in a glacial fjord by tracking breeding individuals from five colonies. Although breeding kittiwakes were observed to travel up to ca. 280 km from the colony, individuals were more likely to use glacier fronts located closer to their colony and rarely used glacier fronts located farther away than 18 km. Such variation in the use of glacier fronts created fine-scale spatial segregation among the four closest (ca. 7 km distance on average) kittiwake colonies. Overall, our results support the hypothesis that spatially predictable foraging patches like glacier fronts can have strong structuring effects on predator movements and can modulate the magnitude of intercolonial spatial segregation in central-place foragers.
Utility of total cell-free DNA levels for surgical damage evaluation in patients with urological surgeries
Authors: Konishi Sakae , Narita Takuma , Hatakeyama Shingo , Yoneyama Tohru , Yoneyama Mihoko Sutoh , Tobisawa Yuki , Noro Daisuke , Sato Tendo , Togashi Kyo , Okamoto Teppei , Yamamoto Hayato , Yoneyama Takahiro , Hashimoto Yasuhiro , Ohyama Chikara
Published: November 2021

The evaluation of surgical damage is challenging because of the lack of specific biomarkers. Total cell-free DNA (cfDNA) levels have been reported to increase with external trauma and may be a biomarker for tissue damage. To investigate the utility of perioperative total cfDNA levels in evaluating surgical damage in urological surgeries. This multicenter, prospective, observational study included 196 patients scheduled for urological surgeries between September 2020 and July 2021. The primary outcome was the change in total cfDNA levels before and after urological surgery. The secondary outcome was the effect of surgical type on total cfDNA ratio before and after urological surgery. The postoperative median total cfDNA level of the 196 patients was significantly increased 2.5-fold compared to the preoperative level (185.2 ng/mL vs. 406.7 ng/mL, P < 0.001). The median total cfDNA before/after ratio was greater than four-fold for kidney transplantation, open cystectomy, and open adrenalectomy. The ratio was less than two-fold for laparoscopic adrenalectomy and robot-assisted radical prostatectomy. Major surgery showed a significant postoperative increase in total cfDNA levels, while minor surgery did not. Total cfDNA levels increased 2.5-fold after urological surgery and it can be used as an acute-phase biomarker for surgical damage.
Detection of blueberry stunt phytoplasma in Eastern Canada using cpn60-based molecular diagnostic assays
Authors: Hammond Christine , Pérez-López Edel , Town Jennifer , Vincent Charles , Moreau Debra , Dumonceaux Tim
Published: November 2021

Blueberry stunt phytoplasma (BBSP; ‘Candidatus Phytoplasma asteris’) is an insect-vectored plant pathogen that causes severe yield losses in blueberry (Vaccinium corymbosum), which is the most valuable fruit crop in Canada. Rapid, field-based diagnostic assays are desirable tools for the control of BBSP, as part of an integrated, proactive approach to production management termed biovigilance. We designed and validated a chaperonin-60 (cpn60)-targeted LAMP assay for detection of BBSP, providing a rapid, low cost, field-deployable diagnostic option. Our validation demonstrates that the assay is reproducible, with high analytical specificity and improved sensitivity when compared with 16S rRNA nested PCR. We applied the validated LAMP assay to nearly 2000 blueberry samples from Québec and Nova Scotia over three growing seasons (2016–2018). Our surveys revealed that BBSP is present in most sites across both provinces, though detection of the pathogen in individual plants varied in different tissues across sampling dates and across years, and evidence of spread between plants was limited. To quantify pathogen load in select plants, we designed additional qPCR and ddPCR assays, also based on cpn60. We found that pathogen load fluctuates in individual plants, both within and between growing seasons. Finally, we designed an interactive map to visualize the results of our surveys. These results provide a validated diagnostic assay that can be used as part of a biovigilance strategy for detecting and controlling infections caused by BBSP.
The first observation of osmotically neutral sodium accumulation in the myocardial interstitium
Authors: Artyukov I. , Arutyunov G. , Bobrov M. , Bukreeva I. , Cedola A. , Dragunov D. , Feshchenko R. , Fratini M. , Mitrokhin V. , Sokolova A. , Vinogradov A. , Gianoncelli A.
Published: November 2021

The aim of this study was the detection and quantification of the Na+ depositions in the extracellular matrix of myocardial tissue, which are suggested to be bound by negatively charged glycosaminoglycan (GAG) structures. The presented experimental results are based on high resolution X-ray fluorescence (XRF) spectromicroscopy technique used to perform a comparative analysis of sodium containment in intracellular and interstitial spaces of cardiac tissues taken from animals selected by low and high sodium intake rates. The experimental results obtained show that high sodium daily intake can result in a remarkable increase of sodium content in the myocardial interstitium.
Chronic pathophysiological changes in the normal brain parenchyma caused by radiotherapy accelerate glioma progression
Authors: Tsuji Yuichiro , Nonoguchi Naosuke , Okuzaki Daisuke , Wada Yusuke , Motooka Daisuke , Hirota Yuki , Toho Taichiro , Yoshikawa Nobuhiko , Furuse Motomasa , Kawabata Shinji , Miyatake Shin-Ichi , Nakamura Hiroyuki , Yamamoto Ryohei , Nakamura Shota , Kuroiwa Toshihiko , Wanibuchi Masahiko
Published: November 2021

Radiation therapy is one of standard treatment for malignant glioma after surgery. The microenvironment after irradiation is considered not to be suitable for the survival of tumor cells (tumor bed effect). This study investigated whether the effect of changes in the microenvironment of parenchymal brain tissue caused by radiotherapy affect the recurrence and progression of glioma. 65-Gy irradiation had been applied to the right hemisphere of Fisher rats. After 3 months from irradiation, we extracted RNA and protein from the irradiated rat brain. To study effects of proteins extracted from the brains, we performed WST-8 assay and tube formation assay in vitro. Cytokine production were investigated for qPCR. Additionally, we transplanted glioma cell into the irradiated and sham animals and the median survival time of F98 transplanted rats was also examined in vivo. Immunohistochemical analyses and invasiveness of implanted tumor were evaluated. X-ray irradiation promoted the secretion of cytokines such as CXCL12, VEGF-A, TGF-β1 and TNFα from the irradiated brain. Proteins extracted from the irradiated brain promoted the proliferation and angiogenic activity of F98 glioma cells. Glioma cells implanted in the irradiated brains showed significantly high proliferation, angiogenesis and invasive ability, and the post-irradiation F98 tumor-implanted rats showed a shorter median survival time compared to the Sham-irradiation group. The current study suggests that the microenvironment around the brain tissue in the chronic phase after exposure to X-ray radiation becomes suitable for glioma cell growth and invasion.
Radial analysis and scaling of urban land use
Authors: Lemoy Rémi , Caruso Geoffrey
Published: November 2021

We determine the functional form and scaling law of radial artificial land use profiles in 300 European functional urban areas (FUAs). These profiles, starting from a fully artificial surface in the city center, decrease exponentially, the faster the smaller the city. More precisely, the characteristic decrease distance scales like the square root of total population, meaning that the artificial surface of cities is proportional to their population. This also means that the amount of artificial land per capita is independent of city size, and that larger cities are not more or less parsimonious in terms of land use than smaller ones.
Dynamics of online hate and misinformation
Authors: Cinelli Matteo , Pelicon Andraž , Mozetič Igor , Quattrociocchi Walter , Novak Petra Kralj , Zollo Fabiana
Published: November 2021

Online debates are often characterised by extreme polarisation and heated discussions among users. The presence of hate speech online is becoming increasingly problematic, making necessary the development of appropriate countermeasures. In this work, we perform hate speech detection on a corpus of more than one million comments on YouTube videos through a machine learning model, trained and fine-tuned on a large set of hand-annotated data. Our analysis shows that there is no evidence of the presence of “pure haters”, meant as active users posting exclusively hateful comments. Moreover, coherently with the echo chamber hypothesis, we find that users skewed towards one of the two categories of video channels (questionable, reliable) are more prone to use inappropriate, violent, or hateful language within their opponents’ community. Interestingly, users loyal to reliable sources use on average a more toxic language than their counterpart. Finally, we find that the overall toxicity of the discussion increases with its length, measured both in terms of the number of comments and time. Our results show that, coherently with Godwin’s law, online debates tend to degenerate towards increasingly toxic exchanges of views.
Neurofilaments can differentiate ALS subgroups and ALS from common diagnostic mimics
Authors: Behzadi Arvin , Pujol-Calderón Fani , Tjust Anton E. , Wuolikainen Anna , Höglund Kina , Forsberg Karin , Portelius Erik , Blennow Kaj , Zetterberg Henrik , Andersen Peter Munch
Published: November 2021

Delayed diagnosis and misdiagnosis are frequent in people with amyotrophic lateral sclerosis (ALS), the most common form of motor neuron disease (MND). Neurofilament light chain (NFL) and phosphorylated neurofilament heavy chain (pNFH) are elevated in ALS patients. We retrospectively quantified cerebrospinal fluid (CSF) NFL, CSF pNFH and plasma NFL in stored samples that were collected at the diagnostic work-up of ALS patients (n = 234), ALS mimics (n = 44) and controls (n = 9). We assessed the diagnostic performance, prognostication value and relationship to the site of onset and genotype. CSF NFL, CSF pNFH and plasma NFL levels were significantly increased in ALS patients compared to patients with neuropathies & myelopathies, patients with myopathies and controls. Furthermore, CSF pNFH and plasma NFL levels were significantly higher in ALS patients than in patients with other MNDs. Bulbar onset ALS patients had significantly higher plasma NFL levels than spinal onset ALS patients. ALS patients withC9orf72HRE mutations had significantly higher plasma NFL levels than patients with SOD1 mutations. Survival was negatively correlated with all three biomarkers. Receiver operating characteristics showed the highest area under the curve for CSF pNFH for differentiating ALS from ALS mimics and for plasma NFL for estimating ALS short and long survival. All three biomarkers have diagnostic value in differentiating ALS from clinically relevant ALS mimics. Plasma NFL levels can be used to differentiate between clinical and genetic ALS subgroups.
Blocked O-GlcNAc cycling alters mitochondrial morphology, function, and mass
Authors: Akinbiyi Elizabeth O. , Abramowitz Lara K. , Bauer Brianna L. , Stoll Maria S. K. , Hoppel Charles L. , Hsiao Chao-Pin , Hanover John A. , Mears Jason A.
Published: November 2021

O-GlcNAcylation is a prevalent form of glycosylation that regulates proteins within the cytosol, nucleus, and mitochondria. The O-GlcNAc modification can affect protein cellular localization, function, and signaling interactions. The specific impact of O-GlcNAcylation on mitochondrial morphology and function has been elusive. In this manuscript, the role of O-GlcNAcylation on mitochondrial fission, oxidative phosphorylation (Oxphos), and the activity of electron transport chain (ETC) complexes were evaluated. In a cellular environment with hyper O-GlcNAcylation due to the deletion of O-GlcNAcase (OGA), mitochondria showed a dramatic reduction in size and a corresponding increase in number and total mitochondrial mass. Because of the increased mitochondrial content, OGA knockout cells exhibited comparable coupled mitochondrial Oxphos and ATP levels when compared to WT cells. However, we observed reduced protein levels for complex I and II when comparing normalized mitochondrial content and reduced linked activity for complexes I and III when examining individual ETC complex activities. In assessing mitochondrial fission, we observed increased amounts of O-GlcNAcylated dynamin-related protein 1 (Drp1) in cells genetically null for OGA and in glioblastoma cells. Individual regions of Drp1 were evaluated for O-GlcNAc modifications, and we found that this post-translational modification (PTM) was not limited to the previously characterized residues in the variable domain (VD). Additional modification sites are predicted in the GTPase domain, which may influence enzyme activity. Collectively, these results highlight the impact of O-GlcNAcylation on mitochondrial dynamics and ETC function and mimic the changes that may occur during glucose toxicity from hyperglycemia.
The human TRAM1 locus expresses circular RNAs
Authors: Dubois Josephine , Sczakiel Georg
Published: November 2021

Numerous indirect and in silico produced evidences suggest circular RNAs (circRNA) in mammals while thorough experimental proofs of their existence have rarely been reported. Biological studies of circRNA, however, should be based on experimentally verified circRNAs. Here, we describe the identification of two circRNAs originating from the gene locus of the translocation associated membrane protein 1 (TRAM1). Linear and potentially circular TRAM1-specific transcripts were identified in a transcriptome analysis of urine RNA of bladder cancer (BCa) patients versus healthy donors. Thus, we first focused on the topology of TRAM1-specific transcripts. We describe conclusive experimental evidence for the existence of TRAM1-specific circRNAs in the human BCa cell lines ECV-304 and RT-4. PCR-based methodology followed by cloning and sequencing strongly indicated the circular topology of two TRAM1 RNAs. Further, studies with exon fusion sequence-specific antisense oligonucleotides (asON) and RNase H as well as studies in the use of RNase R contribute to conclusive set of experiments supporting the circular topology of TRAM1 transcripts. On the biological side, TRAM1-specific circRNAs showed low expression levels and minor differences in BCa cell lines while linear TRAM1 transcripts displayed down-regulated expression in the higher cancer stage model ECV-304 versus more differentiated RT-4 cells.
The Quaternary Kurobegawa Granite: an example of a deeply dissected resurgent pluton
Authors: Ito Hisatoshi , Adachi Yoshiko , Cambeses Aitor , Bea Fernando , Fukuyama Mayuko , Fukuma Koji , Yamada Ryuji , Kubo Takashi , Takehara Mami , Horie Kenji
Published: November 2021

The Quaternary Kurobegawa Granite, central Japan, is not only the youngest known granitic pluton exposed on the Earth’s surface, it is one of few localities where both Quaternary volcanics and related plutons are well exposed. Here, we present new zircon U–Pb ages together with whole rock and mineral geochemical data, revealing that the Kurobegawa Granite is a resurgent pluton that was emplaced following the caldera-forming eruption of the Jiigatake Volcanics at 1.55 ± 0.09 Ma. Following the eruption, the remnant magma chamber progressively cooled forming the voluminous Kurobegawa pluton in the upper crust (~ 6 km depth) until ~ 0.7 Ma when resurgence caused rapid uplift and erosion in the region. This is the first study to document the detailed spatiotemporal evolution of resurgent pluton for a Quaternary caldera system. Our new findings may contribute significantly to understanding the fate of active caldera systems that can produce supereruptions.
Defining skin aging and its risk factors: a systematic review and meta-analysis
Authors: Wong Qi Yi Ambrose , Chew Fook Tim
Published: November 2021

Skin aging has been defined to encompass both intrinsic and extrinsic aging, with extrinsic aging effected by environmental influences and overlaying the effects of chronological aging. The risk factors of skin aging have been studied previously, using methods of quantifying skin aging. However, these studies have yet to be reviewed. To better understand skin aging risk factors and collate the available data, we aimed to conduct a systematic review and meta-analysis. We conducted our systematic review in compliance with Preferred Reporting Item for Systematic Review and Meta-Analyses (PRISMA) guidelines. Embase, PubMed and Web of Science databases were searched in October 2020 using specific search strategies. Where odds ratios were reported, meta-analyses were conducted using the random effects model. Otherwise, significant factors were reported in this review. We identified seven notable risk factors for various skin aging phenotypes: age, gender, ethnicity, air pollution, nutrition, smoking, sun exposure. This review’s results will guide future works, such as those aiming to examine the interaction between genetic and environmental influences.
Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides
Authors: Seni Sushmita , Kaur Satinder , Malik Palvi , Yadav Inderjit Singh , Sirohi Parul , Chauhan Harsh , Kaur Amandeep , Chhuneja Parveen
Published: November 2021

Wheat, one of the major cereal crops worldwide, get adversely affected by rising global temperature. We have identified the diploid B genome progenitor of wheat, Aegilops speltoides (SS), as a potential donor for heat stress tolerance. Therefore, the present work was planned to study the total transcriptome profile of heat stress-tolerant Ae. speltoides accession pau3809 (AS3809) and compare with that of tetraploid and hexaploid wheat cultivars PDW274 and PBW725, respectively. The comparative transcriptome was utilized to identify and validate heat stress transcription factors (HSFs), the key genes involved in imparting heat stress tolerance. Transcriptome analysis led to the identification of a total of 74 K, 68 K, and 76 K genes in AS3809, PDW274, and PBW725, respectively. There was a high uniformity of GO profiles under the biological, molecular, and cellular functions across the three wheat transcriptomes, suggesting the conservation of gene function. Twelve HSFs having the highest FPKM value were identified in the AS3809 transcriptome data, while six of these HSFs namely HSFA3, HSFA5, HSFA9, HSFB2a, HSFB2b, and HSFC1b, were validated with qRT PCR. These six HSFs were identified as an important component of thermotolerance in AS3809 as evident from their comparative higher expression under heat stress.
Antibody-guided design and identification of CD25-binding small antibody mimetics using mammalian cell surface display
Authors: See Kyra , Kadonosono Tetsuya , Miyamoto Kotaro , Tsubaki Takuya , Ota Yumi , Katsumi Marina , Ryo Sumoe , Aida Kazuki , Minegishi Misa , Isozaki Tatsuhiro , Kuchimaru Takahiro , Kizaka-Kondoh Shinae
Published: November 2021

Small antibody mimetics that contain high-affinity target-binding peptides can be lower cost alternatives to monoclonal antibodies (mAbs). We have recently developed a method to create small antibody mimetics called FLuctuation-regulated Affinity Proteins (FLAPs), which consist of a small protein scaffold with a structurally immobilized target-binding peptide. In this study, to further develop this method, we established a novel screening system for FLAPs called monoclonal antibody-guided peptide identification and engineering (MAGPIE), in which a mAb guides selection in two manners. First, antibody-guided design allows construction of a peptide library that is relatively small in size, but sufficient to identify high-affinity binders in a single selection round. Second, in antibody-guided screening, the fluorescently labeled mAb is used to select mammalian cells that display FLAP candidates with high affinity for the target using fluorescence-activated cell sorting. We demonstrate the reliability and efficacy of MAGPIE using daclizumab, a mAb against human interleukin-2 receptor alpha chain (CD25). Three FLAPs identified by MAGPIE bound CD25 with dissociation constants of approximately 30 nM as measured by biolayer interferometry without undergoing affinity maturation. MAGPIE can be broadly adapted to any mAb to develop small antibody mimetics.
Bloodletting has no effect on the blood pressure abnormalities of hyperandrogenic women taking oral contraceptives in a randomized clinical trial
Authors: Luque-Ramírez Manuel , Ortiz-Flores Andrés E. , Nattero-Chávez Lía , Martínez-García M.Ángeles , Insenser María , Álvarez-Blasco Francisco , Fernández-Durán Elena , Quintero-Tobar Alejandra , de Lope Quiñones Sara , Escobar-Morreale Héctor F.
Published: November 2021

Normoferritinemic women with functional hyperandrogenism show a mild iron overload. Iron excess, hyperandrogenism, and cardioautonomic dysfunction contribute to blood pressure (BP) abnormalities in these patients. Furthermore, combined oral contraceptives (COC) prescribed for hyperandrogenic symptoms may worse BP recordings. Iron depletion by phlebotomy appears to lower BP in other acquired iron overload conditions. We aimed to determine the effect of iron depletion on the office BP, ambulatory BP monitoring, and frequency of hypertension in patients with functional hyperandrogenism submitted to standard therapy with COC. We conducted a phase 2 randomized, controlled, parallel, open-label clinical trial (NCT02460445) in adult women with functional hyperandrogenism including hyperandrogenic polycystic ovary syndrome and idiopathic hyperandrogenism. After a 3-month run-in period of treatment with 35 µg ethinylestradiol plus 2 mg cyproterone acetate, participants were randomized (1:1) to three scheduled bloodlettings or observation for another 9 months. Main outcome measures were the changes in office BP, 24-h-ambulatory BP, and frequency of hypertension in both study arms. From June 2015 to June 2019, 33 women were included in the intention-to-treat analyses. We observed an increase in mean office systolic BP [mean of the differences (MD): 2.5 (0.3–4.8) mmHg] and night-time ambulatory systolic BP [MD 4.1 (1.4–6.8) mmHg] after 3 months on COC. The percentage of nocturnal BP non-dippers also increased, from 28.1 to 92.3% (P < 0.001). Office and ambulatory BP did not change throughout the experimental period of the trial, both when considering all women as a whole or as a function of the study arm. The frequency of the non-dipping pattern in BP decreased during the experimental period [OR 0.694 (0.577–0.835), P < 0.001], regardless of the study arm. Decreasing iron stores by scheduled bloodletting does not override the BP abnormalities caused by COC in women with functional hyperandrogenism.
Shifting seasonality of cyclones and western boundary current interactions in Bay of Bengal as observed during Amphan and Fani
Authors: Sil Sourav , Gangopadhyay Avijit , Gawarkiewicz Glen , Pramanik Saikat
Published: November 2021

In recent years, the seasonal patterns of Tropical Cyclones (TC) in the Bay of Bengal have been shifting. While tropical depressions have been common in March–May (spring), they typically have been relatively weaker than the TCs during October–December. Here we show that the spatial pattern of recent warming trends during the last two decades in the southwestern Bay has allowed for stronger springtime pre-monsoon cyclones such as Amphan (May 2020, Super Cyclone) and Fani (April–May 2019, Extremely Severe Cyclone). The tracks of the pre-monsoon cyclones shifted westward, concurrent with an increasing rate of warming. This shift allowed both Fani and Amphan tracks to cross the northeastward warm Western Boundary Current (WBC) and associated warm anti-cyclonic eddies, while the weaker Viyaru (April 2013, Cyclonic Storm) did not interact with the WBC. A quantitative model linking the available along-track heat potential to cyclone’s intensity is developed to understand the impact of the WBC on cyclone intensification. The influence of the warming WBC and associated anti-cyclonic eddies will likely result in much stronger springtime TCs becoming relatively common in the future.
Pollination success increases with plant diversity in high-Andean communities
Authors: Gavini Sabrina S. , Sáez Agustín , Tur Cristina , Aizen Marcelo A.
Published: November 2021

Pollinator-mediated plant–plant interactions have traditionally been viewed within the competition paradigm. However, facilitation via pollinator sharing might be the rule rather than the exception in harsh environments. Moreover, plant diversity could be playing a key role in fostering pollinator-mediated facilitation. Yet, the facilitative effect of plant diversity on pollination remains poorly understood, especially under natural conditions. By examining a total of 9371 stigmas of 88 species from nine high-Andean communities in NW Patagonia, we explored the prevalent sign of the relation between conspecific pollen receipt and heterospecific pollen diversity, and assessed whether the incidence of different outcomes varies with altitude and whether pollen receipt relates to plant diversity. Conspecific pollen receipt increased with heterospecific pollen diversity on stigmas. In all communities, species showed either positive or neutral but never negative relations between the number of heterospecific pollen donor species and conspecific pollen receipt. The incidence of species showing positive relations increased with altitude. Finally, stigmas collected from communities with more co-flowering species had richer heterospecific pollen loads and higher abundance of conspecific pollen grains. Our findings suggest that plant diversity enhances pollination success in high-Andean plant communities. This study emphasizes the importance of plant diversity in fostering indirect plant–plant facilitative interactions in alpine environments, which could promote species coexistence and biodiversity maintenance.
No difference in mid-term outcome after superior vs. anteroinferior plate position for displaced midshaft clavicle fractures
Authors: Nolte Philip-Christian , Tross Anna-Katharina , Studniorz Julia , Grützner Paul-Alfred , Guehring Thorsten , Schnetzke Marc
Published: November 2021

To compare outcomes, complications, revisions, and rates of implant removal of superior compared to anteroinferior plating in displaced midshaft clavicle fractures at mid-term follow-up. We retrospectively reviewed 79 patients who underwent operative treatment for displaced midshaft clavicle fractures (Group A: 28 patients with superior plating; Group B: 51 patients with anteroinferior plating) that were at least 2 years postoperatively. Adjusted Constant Score (aCS), Visual Analog Scale (VAS), and Quick Disabilities of the Arm, Shoulder and Hand (QuickDASH) score were compared. Bone union, implant removal, complications and revision surgeries were assessed. Group A had a significantly higher aCS compared to group B (90, IQR: 85.0–91.0 vs. 91, IQR: 90.0–93.0; P = 0.037). No significant differences between groups were seen in VAS (P = 0.283) and QuickDASH (P = 0.384). Bone union was achieved in 76 patients (96.2%) with no significant differences between groups (Group A: 96.4% vs. Group B: 96.1%; P > 0.999). There were no significant differences in implant removal rates (Group A: 60.7% vs. Group B: 66.7%; P = 0.630), complications (Group A: 46.4% vs. Group B: 31.4%; P = 0.226) and revisions (Group A: 25% vs. Group B: 9.8%; P = 0.102). Superior and anteroinferior plating result in high bone union rates and good clinical outcomes with similar rates of plate removal.
The transition from the female-like great calls to male calls during ontogeny in southern yellow-cheeked gibbon males (Nomascus gabriellae)
Authors: Hradec Michal , Illmann Gudrun , Bartoš Luděk , Bolechová Petra
Published: November 2021

It is well known that gibbons emit a pattern of vocalizations, which is specific for species and sex. A previous study showed, however, that immature southern yellow-cheeked gibbon (Nomascus gabriellae) males produce only female-like great calls from 2.3 to 5.3 years of age in co-singing interactions with their mothers. To date, nothing is known about how the vocal repertoire of a male changes from the female-like call (great call) to the male call (staccato notes and multi-modulation phrase) during vocal ontogeny. The goal of this study was to describe the transition from the female-like great call to the male call and the ontogeny of the male call. We predicted that the transition from the female-like great call to the male-specific call and the development of the male call is a normal part of the aging proces. If this is the case, the following phenomena will occur: (a) female vocalization should no longer be produced with the mature form of the multi-modulation phrase and (b) all stages of the male vocalization should occur gradually as the young male ages. Young males regularly emit both female-like great calls and male-specific calls between the ages of 5.6 to 7.1 years. Once the young males reached 7.1 years of age, they emitted male calls exclusively, and they continued to do so until the end of the observation period (at 8.11 years of age). It was confirmed that the young males emitted only female-like great calls during periods when they produced non-mature forms of a multi-modulation phrase (Fm0,1—none or one frequency modulation in second notes). Furhermore, the decrease in the number of female-like great calls was attributed to the development of the mature form of the multi-modulation phrase (Fm2—two or more frequency modulation in second notes), which developed with age. We also confirmed that the multi-modulation phrase developed gradually, while the development of the staccato notes occurred in leaps. A multi-modulation phrase developed as the initial part of the male-specific call. It was evolved from a simpler to a more complex form as the maximum frequency and age of the young males increased. Staccato notes subsequently developed in certain young males. Possible explanations for such vocal ontogeny in young males are discussed in this work.
Exposing structural variations in SARS-CoV-2 evolution
Authors: Yang Jiaan , Zhang Peng , Cheng Wen Xiang , Lu Youyong , Gang Wu , Ren Gang
Published: November 2021

The mutation of SARS-CoV-2 influences viral function as residue replacements affect both physiochemical properties and folding conformations. Although a large amount of data on SARS-CoV-2 is available, the investigation of how viral functions change in response to mutations is hampered by a lack of effective structural analysis. Here, we exploit the advances of protein structure fingerprint technology to study the folding conformational changes induced by mutations. With integration of both protein sequences and folding conformations, the structures are aligned for SARS-CoV to SARS-CoV-2, including Alpha variant (lineage B.1.1.7) and Delta variant (lineage B.1.617.2). The results showed that the virus evolution with change in mutational positions and physicochemical properties increased the affinity between spike protein and ACE2, which plays a critical role in coronavirus entry into human cells. Additionally, these structural variations impact vaccine effectiveness and drug function over the course of SARS-CoV-2 evolution. The analysis of structural variations revealed how the coronavirus has gradually evolved in both structure and function and how the SARS-CoV-2 variants have contributed to more severe acute disease worldwide.
Exposure of infants to organochlorine pesticides from breast milk consumption in southwestern Ethiopia
Authors: Mekonen Seblework , Ambelu Argaw , Wondafrash Mekitie , Kolsteren Patrick , Spanoghe Pieter
Published: November 2021

Breast milk is often used as an environmental bioindicator since it serves as an important medium to accumulate organochlorine pesticides. The main aim of this study is to determine the level of organochlorine pesticides in human breast milk collected from three districts of southwestern Ethiopia (Asendabo, Deneba, and Serbo) at three times points baselines (1st month), midline ( six months), and end line (12 months) and risk of infants’ exposure. A longitudinal study was conducted to assess pesticide residues in human breast milk samples and evaluate the risk-exposure of infants to these pesticides from consumption of mother’s milk in Ethiopia. Breast milk samples were collected from 168 mothers at three time points and pesticides were extracted using the quick, easy, cheap, effective, rugged, and safe (QuEChERS) method. The level of pesticide residues in human milk samples and exposure of infants to the pesticides was evaluated by calculating the estimated daily intake and compared with the provisional tolerable daily intake of the pesticides under study. The results indicated that, from the eight organochlorine pesticides analyzed in 447 breast milk samples at three sampling times, DDT and its metabolites were detected. p,p′-DDE and p,p′-DDT were detected in all (100%) of the breast milk samples while, p,p-DDD, and o,p-DDT were detected in 53.9%, and 42.7%, respectively. The mean concentration of total DDT at three time points(baseline, midline and endline) were 2.25, 1.68 and 1.32 µg/g milk fat, respectively. The mean concentration of total DDT from the three districts was 1.85 µg/g milk fat which is above the maximum residue limit (MRL = 0.02 µg/g milk fat set by FAO/WHO). The mean ratio of DDT/DDE for the three areas was calculated less than five (< 5) indicates historical DDT use in the study area. The estimated daily intake of infants at the first month of breastfeeding was 11.24 µg/kg-BW/day, above the provisional tolerable daily intake (PTDI) for total DDT set by FAO/WHO, which is 10 µg/kg body weight. An intake of OCPs is a big concern for infants' health in Ethiopia and countries with a similar condition, particularly at the first month of lactation. Strict regulations of the health-threatening pesticide by the regulatory body (Environment, Forest and Climate Change Commission) at the country and regional levels is advocated.
Combined wide-field optical coherence tomography angiography density map for high myopic glaucoma detection
Authors: Kim Yu Jeong , Na Kyeong Ik , Lim Han Woong , Seong Mincheol , Lee Won June
Published: November 2021

The present study aimed to evaluate the diagnostic ability of wide-field optical coherence tomography angiography (OCTA) density map for detection of glaucomatous damage in high myopic (HM) eyes and to further compare the diagnostic ability of OCTA with that of conventional imaging approaches including red-free photography and swept-source OCT (SS-OCT) wide-field maps. A total of 77 healthy HM eyes and 72 HM eyes with open angle glaucoma (OAG) participated in this retrospective observational study. Patients underwent a comprehensive ocular examination, including wide-field SS-OCT scan and peripapillary area and macular OCTA scans. An integrated OCTA density map thereafter was merged by vascular landmark-guided superimposition of peripapillary and macular superficial vascular density maps onto the red-free photography (resulting in the OCTA-PanoMap). Glaucoma specialists then determined the presence of glaucomatous damage in HM eyes by reading the OCTA-PanoMap and compared its sensitivity and specificity with those of conventional images. Sensitivity and specificity of OCTA-PanoMap for HM-OAG diagnosis was 94.4% and 96.1%, respectively. Compared with other imaging methods, the sensitivity of OCTA-PanoMap was significantly higher than that of red-free photography (P = 0.022) and comparable to that of wide-field SS-OCT maps. Specificity of OCTA-PanoMap was significantly higher than those of other conventional imaging methods (except for wide-field thickness map). The OCTA-PanoMap showed good diagnostic ability for discrimination of HM-OAG eyes from healthy HM eyes. As a complementary method of an alternative imaging modality, OCTA-PanoMap can be a useful tool for detection of HM-OAG.
Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice
Authors: Kondo-Takuma Yuko , Mizuno Masayuki , Tsuda Yo , Madokoro Yuta , Suzuki Kengo , Sato Toyohiro , Takase Hiroshi , Uchida Yuto , Adachi Ken-ichi , Hida Hideki , Borlongan Cesario V. , Matsukawa Noriyuki
Published: November 2021

The cholinergic efferent network from the medial septal nucleus to the hippocampus plays an important role in learning and memory processes. This cholinergic projection can generate theta oscillations in the hippocampus to encode novel information. Hippocampal cholinergic neurostimulating peptide (HCNP), which induces acetylcholine (Ach) synthesis in the medial septal nuclei of an explant culture system, was purified from the soluble fraction of postnatal rat hippocampus. HCNP is processed from the N-terminal region of a 186-amino acid, 21-kDa HCNP precursor protein, also known as Raf kinase inhibitory protein and phosphatidylethanolamine-binding protein 1. Here, we confirmed direct reduction of Ach release in the hippocampus of freely moving HCNP-pp knockout mice under an arousal state by the microdialysis method. The levels of vesicular acetylcholine transporter were also decreased in the hippocampus of these mice in comparison with those in control mice, suggesting there was decreased incorporation of Ach into the synaptic vesicle. These results potently indicate that HCNP may be a cholinergic regulator in the septo-hippocampal network.
Identification for antitumor effects of tramadol in a xenograft mouse model using orthotopic breast cancer cells
Authors: Kim Myoung Hwa , Lee Jeong-Rim , Kim Ki-Joon , Jun Ji Hae , Hwang Hye Jeong , Lee Wootaek , Nam Seung Hyun , Oh Ju Eun , Yoo Young Chul
Published: November 2021

In our previous research showed that tramadol having potential anti-tumor effect was associated with enhancement of oncological prognosis in patients with breast cancer surgery. As these effects have not been confirmed by clinical dose-regulated animal or prospective human studies, we investigated the anti-tumor effect of tramadol in vivo. Female nude mice orthotopically inoculated with luciferase-expressing MCF-7 cells, were randomly divided into the control (saline), tramadol group 1 (1.5 mg kg−1 day−1), tramadol group 2 (3 mg kg−1 day−1), and morphine (0.5 mg kg−1 day−1) (n = 5/group). Bioluminescence signals after D-luciferin injection, tumor size, and tumor weight were compared among groups after 4 weeks. Estrogen receptor (ER), progesterone receptor (PR), and transient receptor potential vanilloid (TRPV)-1 expression, natural killer (NK) cell activity, and serum interleukin (IL)-1β, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and interleukin (IL)-6 were then examined. Tumour growth was attenuated in tramadol-treated groups (P < 0.05). NK cell activity was significantly decreased only in the morphine treated group not in sham, control, and tramadol groups. The expression levels of ERα, PRα and β, and TRPV1 were decreased in tramadol group 2 compared with those in the morphine group, but not compared to the control group. Serum levels of IL-6 and TNFα were reduced in both tramadol-treated group 1 and 2 compared to the control group. Overall, clinical dose of tramadol has anti-tumour effects on MCF-7 cell-derived breast cancer in a xenograft mouse model.
An insight into the botanical origins of propolis from permanent preservation and reforestation areas of southern Brazil
Authors: Giovanini de Oliveira Sartori Alan , Papa Spada Fernanda , Pena Ribeiro Victor , Rosalen Pedro Luiz , Ikegaki Masaharu , Kenupp Bastos Jairo , de Alencar Severino Matias
Published: November 2021

Brown propolis from permanent preservation and reforestation areas of southern Brazil have attracted international commercial interest and have a unique composition, although little is known about their botanical origins, which are the plant resins used by bee foragers to produce propolis. Hence, the volatile profiles of organic and non-organic brown propolis and resins of suspected botanical origins—Araucaria angustifolia, Pinus elliott and Pinus taeda—were determined using static headspace gas chromatography coupled to mass spectrometry (SHS-GCMS) and compared. Nighty nine volatiles were tentatively identified, and monoterpenes and sesquiterpenes were the most abundant classes. Principal component analysis (PCA) showed similarity between organic propolis and A. angustifolia volatile profiles (p < 0.05). Hierarchical clustering analysis showed singularities among propolis, even between propolis produced 1 km away from each other. Heatmaps were used to identify peaks present in similar relative intensities in both propolis and conifer resins. Hence, the approach using volatile profiles shed light to propolis botanical origins, which is important for authentication and traceability purposes.
Conversion of methane to acetonitrile over GaN catalysts derived from gallium nitrate hydrate co-pyrolyzed with melamine, melem, or g-C3N4: the influence of nitrogen precursors
Authors: Trangwachirachai Korawich , Chen Chin-Han , Huang Ai-Lin , Lee Jyh-Fu , Chen Chi-Liang , Lin Yu-Chuan
Published: November 2021

The GaN catalyst derived from co-pyrolyzing gallium nitrate hydrate and g-C3N4 is effective in the conversion of methane to acetonitrile because of its well dispersed GaN crystals and amorphous CN species.
Mechanism, reactivity, and selectivity in a palladium-catalyzed organosilicon-based cross coupling reaction
Authors: Wang Qian , Jiang Hui-Mei , Zhuo Shuping , Xu Li-Ping
Published: November 2021

A thermodynamic control mechanism unravels the unusual chemical selectivity in Pd-catalyzed cross-coupling reaction of silacycles.
Modulating the structure and photochromic performance of hybrid metal chlorides with nonphotochromic 1,10-phenanthroline and its derivative
Authors: Xu Fei , Li Gang-Mei , Wang A-Ni , Han Song-De , Pan Jie , Wang Guo-Ming
Published: November 2021

We provide a general method for modulating the structure and photochromic performance of hybrid metal chlorides with 1,10-phenanthroline and its substituted derivative under the synergy of electron transfer and coordination assembly strategies.
Dendrobium fimbriatum Hook polysaccharide ameliorates dextran-sodium-sulfate-induced colitis in mice via improving intestinal barrier function, modulating intestinal microbiota, and reducing oxidative stress and inflammatory responses
Authors: Wang Yu-Jing , Li Qiang-Ming , Zha Xue-Qiang , Luo Jian-Ping
Published: November 2021

The ameliorative effect of Dendrobium fimbriatum polysaccharide (cDFPW1) on ulcerative colitis (UC) was investigated using a dextran-sodium-sulfate-induced (DSS-induced) mouse model in the present study.
Two-dimensional materials for electrochemical CO2reduction: materials,in situ/operandocharacterizations, and perspective
Authors: Chen Zuolong , Wang Xiyang , Mills Joel P. , Du Cheng , Kim Jintae , Wen John , Wu Yimin A.
Published: November 2021

2D materials andin situandoperandocharacterization techniques for the development of next-generation CO2conversion systems.
Synthesis methods of microporous organic polymeric adsorbents: a review
Authors: Khakbaz Mobina , Ghaemi Ahad , Mir Mohamad Sadeghi Gity
Published: November 2021

MOPs can be synthesized in a large variety of ways, which affect their pores and surface area. Variation in synthesis and porosity has a significant effect on their adsorption properties.
Effects of mesoporous silica particle size and pore structure on the performance of polymer-mesoporous silica mixed matrix membranes
Authors: Wang Junhui , Wang Gang , Zhang Zhongshen , Ouyang Gangfeng , Hao Zhengping
Published: November 2021

The effects of filler particle size and pore structure on the gas separation performance of mixed matrix membranes were comprehensively investigated via elaborate synthesis of mesoporous silicas.
Zn2+ detection of a benzimidazole 8-aminoquinoline fluorescent sensor by inhibited tautomerization
Authors: Taş Harun , Adams Jörg , Namyslo Jan C. , Schmidt Andreas
Published: November 2021

The sensor shows a high selectivity and sensitivity toward zinc cations, accompanied by a distinct green fluorescence emission.
Atmospheric pressure chemical vapor deposition growth of vertically aligned SnS2 and SnSe2 nanosheets
Authors: Sierra-Castillo Ayrton , Haye Emile , Acosta Selene , Arenal Raul , Bittencourt Carla , Colomer Jean-François
Published: November 2021

SnS2 and SnSe2 nanosheets were synthesized vertically aligned in different substrates by an AP-CVD method.
Visible photodetector based on transition metal-doped ZnO NRs/PEDOT:PSS hybrid materials
Authors: Nguyen Xuan Hao , Luong Hoai Nhan , Pham Hoang Anh , Nguyen Nhat Minh , Dang Vinh Quang
Published: November 2021

This study reports the fabrication of a hybrid photodetector based on Cu-doped ZnO NRs/PEDOT:PSS, which improves the device's performance and applications.