bluuebunny/crossref_metadata_2025 huggingface dataset:
The Catholic Church in Russia in the Context of the “New Religious Course” of J. Stalin (1944—1946)
Authors: Tokareva Evgenia
Published: January 2025

In 1943 J. Stalin launched the so-called “new religious course”, which was marked by the restoration of the patriarchate and the creation of the Council for the Affairs of the Russian Orthodox Church. In 1944, this course was continued in relation to a number of other confessions and religious movements by the creation of the Council for Religious Cults. However, the Catholic Church in the USSR did not receive any preferences or benefits within the framework of this course. The reason was that, in the opinion of the Soviet authorities, the Catholic and Greek Catholic Churches did not take a patriotic position during the war, sympathized with the German occupiers and even after the end of the war were hostile to the Soviet government, did not justify the hopes that their policies would contribute to the pacification of the local population in the western regions liberated by Soviet troops after mid-1944, and the cessation of partisan actions of anti-Soviet groups. An important factor in this assessment was also the position and role of the Vatican at the end of the war and in the first post-war years, however, due to its extensiveness, this topic is not considered in this article. The article is based on materials from the Council for Religious Affairs (Russian State Archive of Social and Political History, Molotov Fond), the Archive of Foreign Policy of the Russian Federation and the Assumptionist Archive (Rome).
Russia and the Vienna Order: the Discourse of Peace and Security in Post-Napoleonic Europe
Authors: Mirzekhanov Velikhan
Published: January 2025

The author’s focus in this study is on the establishment of the Vienna System of International Relations and the search for peace, security and justice in Europe in the period following the Napoleonic Wars. The sense of fear and the quest for peace, tranquillity and order were expressed through political metaphors, thereby establishing new concepts and ways of thinking about international relations as a basis. The Napoleonic Wars were a period of great uncertainty and instability, in stark contrast to the security, order and tranquillity sought by the European peoples in this new era of peace. The participants involved in the negotiations in Vienna were not seeking to restore the old order, but rather to establish new forms of security and reasonable — including monarchical — rule within the framework of the future new order. This objective was most clearly manifested in the revived rhetoric associated with the “balance of power”, or juste équilibre, as the French referred to it during this period. Following the Congress of Vienna, the European powers established the foundations for safeguarding the status quo and the management of inter-state disputes by means of “political equilibrium”. This assertion, which is frequently endorsed in historiographic discourse, overlooks the broader implications of the Congress. In addition to fostering conditions conducive to the maintenance of the status quo, the European powers also contributed to the emergence of the Pax Europaea and the development of a pan-European security framework, thus reshaping the political landscape on the continent and establishing foundations for enduring European security. The quest for a novel security system was intertwined with a strategic imperative to legitimise the struggle against domestic opposition as well as popular movements within European states. The author concludes that the architects of the Vienna System were able to achieve a consensus approach, relinquishing the “winner-take-all” principle. Thus, they developed a sensible, multilateral mode of diplomacy consistent with the prevailing mood in post-Napoleonic Europe, in the format of conferences and international mediation.
The Heterodox Charm. Althusser and Latin America
Authors: Ortega Jaime
Published: January 2025

This text is a panoramic exercise of revision of the various forms of the reception of Louis Althusser’s work in Latin America. The aim is to verify the intellectual, material and political elements that allowed the diffusion of a very specific conception of Marxism. To a large extent, it is marked by the spirit of renewal that the Marxist intelligentsia had as a project after 1959, when the Cuban revolution made it possible to refound the principles of analysis and rearticulate political hopes. In this sense, the articulating elements of the Althusserian proposal correlate with Latin American diverse social and ideological life. The analysis of the interpretation of Althusserian ideas in the left political thinking on the continent and their impact on political practice constitute the core of this article.
Italian Left-Wing Politician Francesco Di Piazza and His 1964 “Diary”
Authors: Lyubin Valery
Published: January 2025

The published documentary materials cover the life and work of the Italian left-wing politician Francesco Di Piazza (Palermo, Rome, San Gimignano). Of interest are his approaches and assessments of the political situation in the USSR and the prospects for the country's development after the removal of N. S. Khrushchev from his posts, the state of Soviet-Chinese relations, and the situation in the world communist movement. They are contained in the pages of his diary, part of which, with the permission of his son, Marco Di Piazza, is reproduced in this publication. The materials of the “Diary” are supplemented by three other documentary materials: these are the speeches of the authors of this publication - R. Alonzi and V. P. Lubin at the September 2021 celebrations in San Gimignano in honor of the centenary of its famous resident, honorary citizen of this city F. De Piazza, as well as a private letter from Marco Di Piazza to V. P. Lubin, explaining the idea of the full publication of the “Diary” and adding important touches to the portrait of his father. The very way of thinking of Francesco Di Piazza, his interesting and very accurate assessments of life in the USSR, which was perceived by representatives of the left in the mid-twentieth century as a stronghold of socialism, and which many of them oriented themselves to in their political and social activities in their countries, represent important documentary evidence and confirmation of the fact that the developments in the Soviet Union were closely followed in the world, especially among leftists sympathetic to it.
The Scandinavian-Baltic Region in Historical Retrospect. Problems of Studying in Modern and Contemporary Times. All-Russian Scientific Conference. December 5—6, 2024
Authors: Sadova Lyudmila
Published: January 2025

On December 5—6, 2024, the All-Russian conference "Scandinavian-Baltic Region in Historical Retrospect. Problems of Study in Modern and Contemporary Times" was held. It was organized by the Institute of World History of the Russian Academy of Sciences and Lomonosov Moscow State University. Young researchers from leading scholar centres of Moscow, St. Petersburg, Arkhangelsk, Petrozavodsk, Smolensk, Yekaterinburg, Voronezh, Veliky Novgorod, Nizhny Novgorod took part in the conference. Within the work of five sections the participants discussed a wide range of issues related to the history of political and economic development of the Northern and Baltic countries, international relations in modern and contemporary times, from the 15th century to the present day.
Nowy model rodzicielstwa i jego konsekwencje
Authors: Kiszka Monika
Published: January 2025

Artykuł zawiera analizy dotyczące nowego modelu rodzicielstwa, który implikuje współczesne sposoby definiowania macierzyństwa i ojcostwa, wskazując na zachodzącą w tej kwestii paradygmatyczną przemianę. Zawarte w artykule ustalenia obejmują również konsekwencje wynikające z tego modelu dotyczące zarówno samych rodziców, jak i dziecka.
Znaczenie doświadczeń życiowych a preferencje wychowawcze rodziców uczniów w młodszym wieku szkolnym
Authors: Rzewucka Paulina
Published: January 2025

Doświadczenia życiowe wpływają na różne sfery życia. Odgrywają także istotną rolę w kształtowaniu się przekonań związanych z wychowaniem. Przeprowadzone badania empiryczne pokazały, że osobiste doświadczenia życiowe rodziców mają znaczenie dla kształtowania się ich preferencji wychowawczych. Należy też zwrócić uwagę na kategorię własnych doświadczeń bycia wychowywanym jako czynnik mający znaczenie dla kształtowania się preferencji wychowawczych oraz tożsamości rodzica. Rodzina generacyjna jest bowiem pierwszą grupą społeczną, w której człowiek rozpoczyna obserwację otaczającego go świata i nabiera umiejętności funkcjonowania w społeczeństwie. To w niej dziecko obserwuje wzorce rodzicielstwa. Doświadczenia związane z rodziną pochodzenia mogą być inspiracją, a także przyczyną zmian w podejściu do wychowania.
Poczucie sensu życia i wartości młodzieży. Konteksty psychopedagogiczne
Authors: Opozda Danuta
Published: January 2025

Artykuł podejmuje problematykę poczucia sensu życia młodzieży. Kolejno omówiono  zagadnienie rozumienia sensu życia – jego strukturę, funkcje i podmiotowy charakter. Przedstawiono niektóre kwestie związane z systemem wartości i procesami wartościowania. Zwrócono uwagę na filozoficzną proweniencję omawianych wątków. W dalszej części nawiązano do rozwojowych i wychowawczych aspektów kształtowania poczucia sensu życia młodzieży oraz podano przykładowe wyniki badań nad deklarowanymi przez młodzież wartościami mającymi znaczenie w określaniu sensu własnego życia.
Obraz małżeństwa i rodziny w opinii dorosłych dzieci rozwiedzionych rodziców
Authors: Regulska Agnieszka , Ślusarczyk Wiktoria
Published: January 2025

Artykuł przedstawia wybrane wyniki badań, których cel ogólny dotyczył poznania obrazu małżeństwa i rodziny z perspektywy dorosłych dzieci pochodzących z rodzin rozwiedzionych, a także ich opinii na temat cech małżeństwa i rodziny oraz podejścia do rozwodów.
Circumstanses, are Subiected bu the Ascerainment during Investigation of Crimes Commited bu the Medical Workers in the Process of their Professional Activity
Authors:   Saratov State University, Ivanova V. G.
Published: January 2025

The article examines the issues of professional crimes made by medical personnel in hospitals and related establishments. The author focused on one of the structural elements of collecting of evidence – the circumstances needed to be ascertain for the case. Based on the analysis of judicial and other legal norms and regulations article represents the formation of the object of substantiation for such kind of criminal cases.
Las tres triadas de Norberto Bobbio. Coordenadas sobre su pensamiento y sus obras
Authors: Duque Daza Javier
Published: January 2025

Campo de conocimiento: ciencia política. Objetivo: presentar un perfil de la vida y el panorama de las orientaciones temáticas, metodológicas y políticas de Norberto Bobbio, filósofo y politólogo. Metodología: artículo de revisión acerca de la vida y obra del filósofo y politólogo italiano. Se basó en la sistematización y el análisis de artículos biográficos, sus libros autobiográficos, sus numerosos artículos y libros, y las obras de sus críticos. Se elaboró un cuadro general de sus aportes, sus orientaciones metodológicas y sus posiciones acerca de problemas centrales de la filosofía y la ciencia política. Resultado: se presenta el conjunto de su obra, su metodología y su concepción de la sociedad mediante tres triadas: una sobre los campos de conocimiento de su trabajo (filosofía del derecho, filosofía política y ciencia política), otra de sus aspectos metodológicos (la dilucidación conceptual, el uso de las dicotomías y su realismo epistemológico) y la tercera sobre su concepción sobre la sociedad (el liberalismo, el socialismo y el realismo político). Conclusión: del análisis de estas tres dimensiones se resalta la gran huella que dejó el pensamiento y la obra del pensador italiano, especialmente en la ciencia política.
Using of Portfolio Brand Strategy – The Case Study of Łódzki Dom Kultury
Authors: Szymańska Kamila
Published: January 2025

The purpose of this paper is to indicate the usefulness of a brand portfolio management strategy for organizations operating locally in the cultural services market. Brand portfolio strategy is standardly considered useful for international manufacturing companies, which have numerous product lines in their assortment. However, the specificity of the institution such as community center also allows for running in one place many services with separate brands, each of which has its own image and its own group of buyers. The study indicates not only the way of implementing this brand management strategy in the Łódzki Dom Kultury, but also the potential strengths and weaknesses of such a strategic solution in the field of contemporary marketing.
Use of Digital Marketing Tools in Companies from the Financial Sector in the Era of COVID-19 Pandemic
Authors: Dąbrowski Jacek
Published: January 2025

The purpose of the paper is to present the possibilities of using modern technologies in the promotional activities of banks in Poland. This issue has gained particular importance during the development of the COVID-19 pandemic. Already earlier, we could observe an increased openness of consumers to marketing communication involving the use of modern technologies and the Internet, but the pandemic caused the need to reduce direct contact with customers, and thus - to intensify such activities in the Internet sphere. The paper cites examples of digital marketing communication tools that are used by banks operating in Poland.
Mérantis d'Indonésie. D'où venez-vous et comment vous exploite-t-on ?
Authors: Laurent Dominique
Published: January 2025

Dans une première partie l'auteur situe l'importance de l'Indonésie par quelques repères géographiques, économiques et par une présentation rapide du développement de la production, de l'industrie et du commerce des produits forestiers sur les deux dernières décennies en se focalisant, en fin de chapitre, sur Kalimantan.La seconde partie est consacrée à la présentation de l'activité d'exploitation forestière sur terre ferme à Kalimantan : le cadre naturel de la forêt, les entreprises qui y opèrent (leur organisation, leur structure, leur importance, leur méthode), les techniques d'exploitation qu'elles mettent en œuvre et le type de matériel qu'elles utilisent.Un chapitre présente les méthodes et techniques du transport fluvial des Meranti et autres bois flottables produits à partir de cette forêt.La productivité et les prix de revient de ce type d'exploitation et de transport fluvial font l'objet d'un chapitre spécial.La troisième partie, dans un souci d'élargissement de la perspective, rappelle quels sont les objectifs et les axes majeurs de la politique indonésienne de développement de la production, de la transformation et du commerce de ses produits forestiers, tout en indiquant les besoins que cette volonté sous-tend.
The tactical urbanism of urban collectives in Campinas for innovation districts
Authors: Carvalho Laura Martins de , Souza Zilma Borges
Published: January 2025

Based on the theoretical framework of Knowledge-Based Urban Development (KBUD), tactical urbanism, and smart cities, this article aims to explore the relationship between urban collectives in Campinas with the public sector, private sector, and the university. The focus is on their perception of the International Hub for Sustainable Development (HIDS), the Innovation and Sustainable Development Hub (PIDS); the use of Information and Communication Technologies (ICTs) to promote their activities; technology parks and responsible innovation, in order to explore aspects that favor or hinder the development of quintuple helix innovation districts, where the participation of civil society and the integration of the environment are central elements to the innovation process. The research is qualitative and exploratory in nature, and the methodology used is participatory research, involving the following collectives: Coletivo Cuidadores da APP do Jardim Miriam, Brigada Popular Cachorro do Mato, Pé na Estrada Bike Clube, and Mobiliza Satélite. The results show that the collectives possess socio-environmental knowledge and engagement, despite the lack of effective support from the public sector, universities, or the private sector. There is an interest in greater involvement with universities, especially in the development of technologies with social and environmental impact. In this context, the collectives demonstrate potential to implement the quintuple helix innovation model. The results also highlight conflicts between collectives and the public sector, emphasizing the importance of creating permanent participatory forums to ensure democratic management in the territories of the collectives and their participation in innovation districts.
A new approach to assessing the efficacy of anthelmintic agents in vitro
Authors: Perina E. A. , Buyko E. E. , Kaminskiy I. P. , Sobakin D. S. , Ufandeev A. A. , Kaidash O. A. , Ivanov V. V. , Udut E. V.
Published: January 2025

Aim. To develop a new method to determine the viability of Opisthorchis felineus in vitro using the MTS reagent and to evaluate its applicability for analyzing the efficacy of anthelmintic agents in the treatment of opisthorchiasis.

Materials and methods. Golden hamsters were used to create a model of O. felineus infection. The animals were infected with metacercariae obtained from fish of the Cyprinidae family. Three months after infection, adult parasites were extracted from the hepatobiliary system. Their viability was assessed using the motility scale and a new method based on the modified MTS test protocol. To account for differences between the size and number of adult parasite cells, the results were normalized with respect to protein content. To evaluate the feasibility of the new approach in the study of pharmacological activity against opisthorchiasis, the viability of adult parasites in the presence of praziquantel was tested.

Results. During incubation of adult flukes in a medium with the addition of the MTS reagent, colored watersoluble formazan was accumulated. Thermal inactivation of parasites significantly decreased the production of this compound. Since the studied adult parasites differed in size and number of cells, the obtained data on their viability were normalized to protein content. The results correlated with the data on parasite viability obtained by the traditional method using the motility scale. Evaluation of praziquantel efficacy at different concentrations using two independent methods (the MTS test and the motility scale) showed that the results of the MTS test were consistent with literature data and comparable with the results obtained using the motility scale.

Conclusion. A new method for in vitro evaluation of anti-opisthorchiasis activity of drugs was developed. It is based on the assessment of water-soluble formazan production by adult O. felineus flukes in the culture medium using the MTS reagent for screening anti-opisthorchiasis activity of new anthelmintic drugs.
The use of three-dimensional bioprinting for skin regeneration and wound healing (literature review)
Authors: Barsuk I. A. , Golovko K. P. , Alexandrov V. N. , Khasanov A. R. , Edgeev N. I. , Galiullin R. I.
Published: January 2025

Three-dimensional (3D) bioprinting is rapidly proliferating across many medical disciplines and is making strides towards manufacturing intricate human organs for clinical application. One of the most promising areas in 3D bioprinting is development of bioinks with certain composition and designed properties.

The aim of this systematic review was to assess current biomedical research evidence regarding the efficacy of 3D bioprinting for skin regeneration and wound healing. A comprehensive search for all applicable original articles was conducted according to pre-established eligibility criteria. The study employed PubMed, Web of Science, Scopus, Medline Ovid, and ScienceDirect databases.

Of the retrieved articles, eighteen satisfied the inclusion criteria, while twenty-three were excluded. A total of 159 animals that had wound defects were considered in all animal-based research. Collagen and gelatin hydrogels were the most commonly employed bioinks. In relation to cellular composition, allogeneic fibroblasts and keratinocytes were predominant. The observation period ranged from one day to six weeks. Complete wound closure was achieved within 2–4 weeks in most animal studies. In vitro and in vivo animal studies have shown a positive effect of printed bioengineered constructs in accelerating wound healing. Notably, the research where bioprinting was performed directly in the wound in situ was of particular interest. Further studies are required to enhance the tissue bioprinting technique to address skin wound healing in animal models. The utilization of standardized parameters may pave the way for human clinical studies.
Clinical trials on oncolytic viruses
Authors: Golovinov I. V. , Goncharova A. S. , Shulga A. A. , Vlasov S. N. , Dimitriadi S. N.
Published: January 2025

Oncolytic viruses (OVs) are a new class of targeted anticancer drugs with unique mechanisms of action. Oncolytic virotherapy has evolved from the use of in vitro-passaged strains (first generation) to genetically engineered viruses with increased selectivity (second generation) and, ultimately, to recombinant OVs expressing a transgene (third generation).

The aim of the review was to analyze and summarize data on the current state of clinical research on OVs.

A PubMed search identified 182 articles from 1997 to 2024 with 154 studies reporting data on 4,850 patients. We found that adenovirus (n = 44) is the most common OV in clinical trials with more than two-thirds (n = 108) using modified or recombinant viral backbones, and granulocyte-macrophage colony-stimulating factor (GM-CSF; n = 40) was the most common transgene. The most common tumors targeted were melanoma (n = 1,997) and gastrointestinal (GI; n = 916) cancers with the most common monotherapy received by intratumoral (n = 3,003) or intravenous (n = 1,318) delivery routes. The most common combination included chemotherapy (n = 54).

Treatment-related adverse events included low-grade constitutional symptoms and local injection site reactions. Measurements of virus shedding were frequently performed, but many studies were limited to blood and tumor tissue analysis, using only polymerase chain reaction (PCR). Although most studies reported antiviral antibody titers (n = 101), only a few reported virus-specific T-cell responses (n = 23). Objective responses were recorded in 458 (9.4%) patients and disease control was achieved in 1,141 (23.5%) patients, although standard reporting criteria were used in only 60.4% of cases.

These data provide an insight into the current state of clinical research on OVs and highlight potential areas requiring further investigation to better define the role of OVs in cancer treatment.
Markers of gastrointestinal diseases
Authors: Ostanko V. L. , Kalacheva T. P. , Kulumaeva K. A. , Li N. A. , Beloborodova E. V. , Purlik I. L. , Kalyuzhina E. V. , Brazovskaya N. G. , Kalyuzhin V. V.
Published: January 2025

The lifestyle of people nowadays and poor diet are factors affecting the increasing incidence of digestive diseases in people all over the world. The search for new methods of early diagnosis of the disease is an urgent issue of modern medicine. In the last decade, much attention has been paid to various biological markers that can be used to assess the risk of disease development, the response to therapy, and the possible development of complications. Biomarkers in clinical medicine can be used as additional tools not only to improve early diagnosis of gastrointestinal diseases but also to assess the effectiveness of therapy.

The aim of this lecture was to analyze and systematize biomarkers in various gastrointestinal diseases.
The importance of biodiversity of human microbiota and environment in the susceptibility to the development of bronchial asthma in children
Authors: Sokolova T. S. , Malchuk V. N. , Nogai A. A. , Fedorova O. S. , Ogorodova L. M.
Published: January 2025

Bronchial asthma (BA) remains one of the most common chronic respiratory diseases in childhood. BA develops with a combination of genetic predisposition and environmental factors. Epidemiological data on the development of BA emphasize the role of early-life microbiota in the formation of immune responses and susceptibility to the development of BA. In recent years, enough data has been accumulated to suggest that an imbalance in intestinal and airway microbiota during early life may predispose a child to the development of BA. In turn, the biodiversity of the environment influences the colonization of various biotopes in the human body by microorganisms. The study of the mechanisms of interaction between microbiota communities of the environment and humans will pave the way for the development of new strategies for the prevention of BA.

The aim of this review was to analyze current research aimed at assessing the importance of biodiversity of human microbiota and environment in the susceptibility to the development of BA in children.
A model of chronic thromboembolic pulmonary hypertension with the use of microencapsulated fibrin particles
Authors: Karpov A. A. , Shilenko L. A. , Vaulina D. D. , Sidorova E. E. , Akhmetova A. A. , Bunenkov N. S. , Vorotilov A. V. , Ivkin D. Yu. , Karpenko V. V. , Galagudza M. M.
Published: January 2025

Aim. To develop a model of chronic thromboembolic pulmonary hypertension (CTEPH) in rats by embolization of the pulmonary vascular bed with microencapsulated fibrin (MF).

Materials and methods. Microencapsulated fibrin (MF) was prepared by encapsulating fibrin particles smaller than 71 μm in sodium alginate. Non-encapsulated fibrin with a particle size of 71–200 µm was used as an alternative embolic particle. Modeling was performed on male Wistar rats. The animals were divided into 4 groups. Intact (INT) animals (n = 7) were administered normal saline intravenously. In the NF8 group (n = 14), non-encapsulated fibrin was injected as embolic particles 8 times every 4 days. In the MF5 group (n = 14), 0.5 ml MF (9,047 ± 430 particles) was administered intravenously 5 times every 5 days. In the MF8 group (n = 14), MF was administered 8 times every 4 days. Six weeks after the last injection of embolic particles, cardiac catheterization with manometry and histologic examination of the lungs were performed.

Results. According to cardiac catheterization, right ventricular systolic pressure (RVSP) in the MF8 group was significantly higher compared to rats from the INT and NF8 groups (p < 0.05). The hypertrophy index and the percentage of collagen fibers in the structure of the vascular wall of the pulmonary artery branches were significantly higher in the MF5 and MF8 groups than in the INT and NF8 groups (p < 0.01). There were no significant differences between the MF5 and MF8 groups.

Conclusion. A representative CTEPH model in rats was developed, characterized by a stable increase in RVSP and pronounced structural changes in the branches of the pulmonary artery.
Changes in VEGFR1 and VEGFR2 expression and endothelial cell maturity in laboratory animals with a model of Alzheimer’s disease
Authors: Kukla M. V. , Averchuk A. S. , Stavrovskaya A. V. , Rozanova N. A. , Berdnikov A. K. , Kolotyeva N. A. , Salmina A. B.
Published: January 2025

Aim. To evaluate the expression of VEGFR1 and VEGFR2 and the maturity of endothelial cells in neurogenic niches in the model of Alzheimer’s disease.

Materials and methods. The study was carried out on 6-month-old male C57BL/6 mice. The experimental group (n = 15) received 2 µl of 1 mM Aβ25-35 solution in the CA1 hippocampal region, while the control group (n = 15) received normal saline. Brain plasticity was assessed at day 10, 17, and 38 after surgery by the passive avoidance test. The expression of VEGFR1, VEGFR2, and CLDN5 was assessed by immunohistochemistry and the Image ExFluorer imaging system.

Results. In the control group, cognitive training stimulated angiogenesis in the neurogenic niches of the brain, which was accompanied by the formation of microvasculature with fully mature endothelium. In the experimental group, an early and pronounced increase in the VEGFR1 expression was observed by day 7 after cognitive training, which was followed by impaired barrier formation and high VEGFR2 expression by day 28 after cognitive training. These changes were associated with the formation of small vessels with structural incompetence of endothelial cells.

Conclusion. Angiogenesis in neurogenic niches of the animals with the model of Alzheimer’s disease is characterized by incompetent mechanisms regulating the subpopulation composition of endothelial cells, impaired stabilization of the endothelial layer, and a decrease in the maturation rate of endothelial cells in newly formed microvessels by the time of cognitive deficit manifestation. This may contribute to microcirculatory dysfunction and impaired neurogenesis in neurogenic niches as well as to the development of pathological permeability and neuroinflammation. On the whole, the disruption of angiogenesis in neurogenic niches observed in the animal model of Alzheimer’s disease suggests a potential contribution of this mechanism to the development of aberrant brain plasticity.
Dynamic changes in RNA integrity, gene expression, and tissue pathomorphology of experimental mice in the postmortem period
Authors: Buyko E. E. , Perina E. A. , Vasilchenko D. V. , Tsydenova I. A. , Khmelevskaya E. S. , Ufandeev A. A. , Kaidash O. A. , Ivanov V. V. , Vtorushin S. V. , Udut E. V.
Published: January 2025

Aim. To examine the pattern of morphological changes, RNA quality number, and gene expression in mouse tissues sampled at autopsy under controlled experimental conditions.

Materials and methods. Balb/c mice were euthanized and subsequently subjected to necropsy at 0, 3, 12, 24, 48, and 72 hours of the postmortem period. During the first three hours following euthanasia, the mice were maintained at room temperature, after which they were transferred to a refrigerator (4 º С). Total RNA was extracted from tissue samples taken from the kidney, liver, and brain; the integrity of the RNA samples was assessed by capillary electrophoresis, and the RNA quality number (RQN) was calculated. The expression levels of Actb, Epas1, and Rps18 housekeeping genes were evaluated by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) with original primers and probes using the TaqMan assay. The histologic examination was performed according to standard techniques.

Results. Degradation of RNA extracted from mouse kidney tissues appeared to be greater than that of RNA taken from the liver. In the meantime, a negative linear correlation was observed between RQN and the duration of the postmortem interval for liver and kidney samples. In contrast, no significant changes in the RQN score were observed for brain RNA samples at any of the time points. The expression of the Epas1 and Rps18 genes was significantly decreased in mouse kidney and liver tissues. However, the level of Epas1 and Rps18 gene expression in the brain remained stable at all time points and did not exhibit a significant decrease at 72 hours after euthanasia. No obvious morphological changes were detected by the histologic examination, which does not exclude the presence of ultrastructural pathological changes.

Conclusion. RQN in autopsy tissues serves as a crucial predictor of sample quality for molecular biology studies, including gene expression analysis.
Phenotypic profile of blood monocytes and tumor-associated macrophages in relation to the expression of galectins 1 and 3 in colorectal cancer
Authors: Kurnosenko A. V. , Reingardt G. V. , Poletika V. S. , Kolobovnikova Yu. V. , Chumakova S. P. , Urazova O. I. , Grishchenko M. Yu. , Churina E. G. , Gamirova K. A.
Published: January 2025

Aim. To identify the features of the subpopulation composition of blood monocytes and tumor macrophages in relation to the plasma concentration and intratumoral expression of galectins 1 and 3 in patients with colorectal cancer.

Materials and methods. A total of 23 patients with colorectal cancer (ICD C18-20) were examined – 5 men and 18 women (average age 63.8 ± 9.4 years). The control group consisted of healthy volunteers; the comparison group encompassed age- and sex-matched patients with colon adenomas. The study materials included whole blood and tumor biopsies. The concentration of galectins 1 and 3 in the blood was determined by enzyme-linked immunosorbent assay, the content of tumor galectin-1+ and galectin-3+ cells – by immunohistochemistry. Subpopulations of blood monocytes were evaluated by flow cytometry; the macrophage immunophenotypes M1 (CD68+ CD80+ ) and M2d (CD68+ CD206+ ) in tumor tissues were determined using immunofluorescence staining. Statistical processing of the research results was performed by the Jamovi 2.3.21 software package for Windows.

Results. In patients with colorectal cancer (CRC), a positive relationship was identified between high plasma concentrations of galectins 1 and 3 and an imbalance of blood monocytes manifested by a decrease in the relative count of classical CD14++CD16- monocytes and, conversely, an increase in the number of non-classical CD14+CD16++ and intermediate CD14+ CD16- cells. The relative numbers of M1 (CD68+CD80+) and M2d (CD68+CD206+) macrophages in CRC tissue samples turned out to be comparable and did not depend on the level of galectins 1 and 3 in the blood and tumor. In patients with colon adenomas, the M2d subpopulation of tumorassociated macrophages was predominant (p = 0.031).

Conclusion. In patients with CRC, galectins 1 and 3 have a modulating effect on the ratio of non-classical CD14+CD16++, intermediate CD14+CD16- , and classical CD14++CD16- monocytes in the blood and do not affect the M1/M2d expression profile of tumor-associated macrophages.
Predictors of mortality in hospitalized patients with COVID-19
Authors: Malinovskiy V. A. , Fedosenko S. V. , Semakin A. V. , Dirks I. I. , Arzhanik M. B. , Semenova O. L. , Vinokurova D. A. , Starovoitova E. A. , Agaeva S. A. , Nesterovich S. V. , Kalyuzhin V. V.
Published: January 2025

Aim. To determine clinical and laboratory factors associated with a severe course and lethality in hospitalized patients with COVID-19.

Materials and methods. A retrospective comparative study included data of 745 adult patients hospitalized with COVID-19 from 16.05.2020 to 30.09.2020 (Tomsk, Russia). The intergroup comparison of indices, ROC analysis, and determination of odds ratio to assess the association between risk factors and the outcome were performed.

Results. Age > 62 years, pneumonia within a year before COVID-19, and the presence of ≥3 comorbidities were associated with a fatal outcome (FO). Negative predictors of the outcome at the time of hospitalization included dyspnea, diastolic blood pressure ≤ 80 and pulse pressure > 48 mmHg, SpO2 < 94% (and/or a decrease to ≤ 89% throughout hospitalization). Laboratory predictors of FO at admission were platelets ≤ 183 × 109 / l, neutrophils > 4.57 × 109 / l, lymphocytes ≤ 1.08 × 109 / l, neutrophil-to-lymphocyte ratio > 4.8, aspartate aminotransferase > 39 U / l, urea > 6.75 mmol / l, lactate dehydrogenase > 219 U / l, blood albumin ≤ 38 g / l, C-reactive protein (CRP) > 47 mg / l. When threshold values were reached during any of the hospitalization periods, FO was associated with CRP > 38 mg / l, ferritin > 648.6 µg / l, D-dimer > 731.11 ng / ml, white blood cells > 14.27 × 109 / l, lymphocytes ≤ 0.73× 109 / l, duration of oxygen therapy > 3 days, need for non-invasive and invasive ventilation ≥ 1 day, need for glucocorticoid administration > 1 day, reaching a total course dose > 6 mg for dexamethasone.

Conclusion. The factors associated with FO in hospitalized patients with COVID-19 were identified.
Late-season source limitation practices to cope with climate change: delaying ripening and improving colour of Cabernet-Sauvignon grapes and wine in a hot and arid climate
Authors: Pallotti Luca , Partida Guadalupe , Laroche-Pinel Eve , Lanari Vania , Pedroza Miguel , Brillante Luca
Published: January 2025

The current rise in temperatures is hastening grape ripening, resulting in unbalanced wines with high alcohol content but poor colour, aroma and phenolic composition. Late-season canopy manipulation practices aimed at reducing the size of the photosynthetic apparatus after veraison have been shown to delay ripening in cooler climates. However, these methods have not directly been compared in regions with high irradiance and temperature, where berry composition is negatively affected by late fruit exposure.
In this two-year Cabernet-Sauvignon trial, we compared the application of pinolene (P) to late shoot topping (T) and late above-bunch zone leaf removal (LR), all performed during the late stages of ripening, with an untreated control (C). Vine water status was monitored weekly from the time of treatment application until harvest. Gas exchange was monitored only in 2021, from treatment application through to harvest. Berry samples were collected to measure berry weight, total soluble solids, pH, titratable acidity, and the anthocyanin profile. At harvest, we assessed yield components, rated sun damage on clusters, and produced experimental wines. Wine colour and chemical characteristics were evaluated.
The application of P scarcely improved gas exchange and stem water potential. Conversely, LR and T increased water potential but did not affect leaf gas exchange. T was the only treatment that significantly decreased sugar accumulation, followed by P, which showed some effect although not significant. Grape yield was not altered, despite the use of P resulted in a lower berry weight in 2022. Canopy manipulation increased lighting conditions in the canopy and caused a significant presence of damaged fruits, especially in LR. Berry anthocyanins were not affected, but LR resulted in a lower concentration of total anthocyanins in the wine and altered the anthocyanin profile. P increased wine acidity, while LR and T improved wine colour.
This study suggests that late-season canopy management can effectively control ripening speeds and improve the composition of grapes and wines. However, it is essential to avoid grape overexposure by employing the appropriate techniques to ensure grape quality is not compromised.
Factors influencing user interest in using Sharia-compliant Fintech services: A case study of SyarQ in Yogyakarta, Indonesia
Authors: Yuniati Tri , Andriansyah Yuli , Martins José Duarte Moleiro
Published: January 2025

IntroductionThe rapid growth of financial technology has led to the emergence of sharia-compliant services like SyarQ, an Islamic-based online installment platform. Understanding the factors that influence consumer interest in such services is essential for their adoption and success.ObjectivesThis study aims to analyze the factors affecting users' interest in using SyarQ's services by integrating the Technology Acceptance Model (TAM) and the Theory of Planned Behavior (TPB).MethodA quantitative and exploratory research design was employed, utilizing online questionnaires distributed to 100 verified SyarQ users. The data were analyzed using Partial Least Squares Structural Equation Modeling (PLS-SEM) to test the proposed hypotheses and examine the relationships between variables.ResultsThe findings reveal that user attitude has a positive and significant effect on the interest in using SyarQ services. Perceived behavioral control positively influences perceived ease of use but does not significantly affect interest directly. Perceived ease of use positively affects both attitude and perceived usefulness. Subjective norms have a positive and significant impact on perceived usefulness but do not significantly influence interest. Perceived usefulness does not have a significant effect on attitude or interest.ImplicationsThe study suggests that enhancing user attitudes and simplifying the user experience can increase consumer interest in Sharia-compliant fintech services like SyarQ. Providers should focus on improving the ease of use and addressing factors that positively influence user attitudes to attract and retain customers.Originality/NoveltyThis research contributes to the existing literature by combining TAM and TPB to examine user interest in a Sharia-compliant fintech platform. It offers valuable insights into consumer behavior within Islamic financial services, a relatively underexplored area.
Obligatory Necessity Theory: Elements and Facts Influenced by the Intensity of the Specific Physical Concept
Authors: Cardoso Carlos Eduardo Ramos
Published: January 2025

The theory says that the first things to exist in the universe and the current moment were driven by an imperative need; that is, the intensities of the physical concept specify influence the existence of elements and facts based on the countless observations in physical spaces in relation to elements and facts. The existence of mandatory elements in space is motivated by the characteristics of the spaces that offer conditions for such an obligation. The theory states that the universe did not arise by chance; that is, the universe emerged through determination influenced by its surroundings. The objective of the study is to help explain the elements and facts of the universe from its origins to the present.
Best digital practices in Mental Health: Design and Implementation of a Tailored Mobile Application for Vulnerable Populations in the European Union
Authors: Torrents Enric Garcia
Published: January 2025

This paper outlines the ongoing development of a specialised mobile application to address the mental health needs of vulnerable populations in the European Union (EU). Conducted as a mixed-methods research within two EU Science and Technology Cooperation Actions, the study prioritises stakeholder feedback to identify features for initial testing and strategies to improve application uptake, using critical digital health analysis. With a focus on marginalised groups such as refugees, migrants and socio-economically disadvantaged individuals, the application aims to provide an accessible and culturally sensitive platform for mental health support, integrating evidence-based interventions such as cognitive behavioural techniques and mindfulness exercises. Key features include multilingual support, habit tracking, personalised recommendations for support and services, and real-time crisis intervention. Collaboration with stakeholders and ongoing rigorous usability testing are driving the refinement process to ensure usability, acceptability and effectiveness, underscoring the importance of user engagement in technology-based mental health interventions in the EU context and highlighting the importance of collaborative science and technology for societal benefit.
Predicting the Occurrence of Falls Among Portuguese Community-Dwelling Adults Aged 50–60, 60–70, and 70 or Older Using the Timed Up and Go Test
Authors: Martins Anabela Correia , Moreira Juliana , Silva Catarina , Tonelo Cláudia , Rocha Clara
Published: January 2025

Falls are a major cause of morbidity and mortality among older adults. While the Timed Up and Go (TUG) test has recently been identified as the best predictor of falls, it should not be used in isolation to identify individuals at risk. This study aims to develop a predictive model by combining the TUG test with other risk factors associated with falls in Portuguese community-dwelling adults aged 50–60, 60–70, and 70 years or older. A total of 403 participants aged 50 or older completed a questionnaire on demographic information and fall risk factors, underwent the TUG test, and were monitored for 12 months to record falls. ROC curve analysis demonstrated that the TUG test alone effectively distinguished fallers from non-fallers exclusively among adults aged 50–60, with a cut-off time of 6.9 seconds. Multivariate logistic regression defined three predictive models based on age groups, with ROC curve results as follows: 50–60 (AUC=0.825, cut-off=18.1), 60–70 (AUC=0.754, cut-off=17.8), and 70 or older (AUC=0.708, cut-off=24.8). These findings are clinically significant, demonstrating that the TUG test combined with a few self-reported questions can efficiently identify individuals at risk of falling in just a few minutes, without requiring specialized equipment.
Maximizing the Impact of Scientific Conferences: A Scoping Review of Methods, Strategies and Future Directions
Authors: Zary Nabil , Alali Mohammed
Published: January 2025

Introduction: Scientific conferences are vital for sharing knowledge, fostering collaboration, and advancing professional development. However, traditional formats are facing increasing challenges related to accessibility, sustainability, and engagement. This scoping review synthesizes current evidence on strategies for organizing scientific conferences to enhance their impact across key dimensions: learning, networking, inclusion, and environmental considerations sustainability.Methods: We conducted a systematic search across Web of Science, Scopus, PubMed, and Google Scholar, analyzing 110 studies published between 1983 and 2024. Two independent reviewers extracted and analyzed data on conference formats, outcomes, and implementation strategies. The review adhered to established scoping review methodologies, including PRISMA guidelines, to ensure comprehensive and transparent reporting.Results: Virtual and hybrid conference formats improved accessibility significantly and reduced carbon emissions by 60–90% compared to traditional in-person formats. However, these formats required careful design to maintain networking benefits, successfully incorporating structured small-group sessions, AI-powered matchmaking, and multi-hub models that combine regional in-person gatherings with global virtual connections. Interactive session designs and asynchronous content access consistently enhanced learning outcomes across all formats. Multi-hub models emerged as a promising compromise, reducing emissions by 60–70% while preserving networking opportunities.Discussion: The evidence indicates that future conference designs should embrace flexible, multi-modal approaches to leverage the strengths of various formats while overcoming participation barriers. Virtual and hybrid formats provide substantial benefits in accessibility and sustainability but necessitate intentional design to ensure effective networking and engagement. Additional research is essential to establish standardized impact metrics and assess the long-term career effects of different conference models. This review offers evidence-based guidance for conference organizers and emphasizes the need for ongoing innovation in conference design to address the evolving requirements of the global academic community.