bluuebunny/crossref_metadata_2025 huggingface dataset:
Rock image classification using deep residual neural network with transfer learning
Authors: Chen Weihao , Su Lumei , Chen Xinqiang , Huang Zhihao
Published: January 2023

Rock image classification is a significant part of geological research. Compared with traditional image classification methods, rock image classification methods based on deep learning models have the great advantage in terms of automatic image features extraction. However, the rock classification accuracies of existing deep learning models are unsatisfied due to the weak feature extraction ability of the network model. In this study, a deep residual neural network (ResNet) model with the transfer learning method is proposed to establish the corresponding rock automatic classification model for seven kinds of rock images. ResNet34 introduces the residual structure to make it have an excellent effect in the field of image classification, which extracts high-quality rock image features and avoids information loss. The transfer learning method abstracts the deep features from the shallow features, and better express the rock texture features for classification in the case of fewer rock images. To improve the generalization of the model, a total of 3,82,536 rock images were generated for training via image slicing and data augmentation. The network parameters trained on the Texture Library dataset which contains 47 types of texture images and reflect the characteristics of rocks are used for transfer learning. This pre-trained weight is loaded when training the ResNet34 model with the rock dataset. Then the model parameters are fine-tuned to transfer the model to the rock classification problem. The experimental results show that the accuracy of the model without transfer learning reached 88.1%, while the model using transfer learning achieved an accuracy of 99.1%. Aiming at geological engineering field investigation, this paper studies the embedded deployment application of the rock classification network. The proposed rock classification network model is transplanted to an embedded platform. By designing a rock classification system, the off-line rock classification is realized, which provides a new solution for the rock classification problem in the geological survey. The deep residual neural network and transfer learning method used in this paper can automatically classify rock features without manually extracting. These methods reduce the influence of subjective factors and make the rock classification process more automatic and intelligent.
Landslide susceptibility assessment using the certainty factor and deep neural network
Authors: Ma Wenli , Dong Jianhui , Wei Zhanxi , Peng Liang , Wu Qihong , Wang Xiao , Dong Yangdan , Wu Yuanzao
Published: January 2023

Areas with vulnerable ecological environments often breed many geological disasters, especially landslides, which pose a severe threat to the safety of people’s lives and property in these areas. To aid in landslide prevention and mitigation, an approach combining the coefficient of determination method (CF) and a deep neural network (DNN) were proposed in this study for landslide susceptibility evaluation. The deep neural network can excavate the deep features of samples and improve the accuracy of the susceptibility model. In addition, the logistic regression model (LRM) and support vector machine (SVM) were selected to create landslide susceptibility maps for comparison, which also involved the coefficient of determination method (CF). Based on landslide remote sensing interpretation and field investigations, a spatial database of mudstone landslides in the Xining area was established. Eight different conditional factors, including the elevation, slope, slope aspect, undulation, curvature, watershed, distance from a fault, and distance from a road, in the study area were selected as the evaluation factors to evaluate the susceptibility. The results revealed that four factors (i.e., the ground elevation, curvature, distance from a fault, and distance from a road) had relatively significant influences on the landslide susceptibility in the study area. Finally, the confusion matrix was used to evaluate the accuracy of the results obtained using the three methods, and the optimal result was selected to evaluate the landslide susceptibility in the study area. It was found that the combined CF-DNN method was more suitable for evaluating the landslide susceptibility in this area. Landslide susceptibility zoning was conducted to divide the study area into four sensitivity levels: low (32.65%), medium (35.12%), high (22.44%), and extremely high (9.79%) susceptibility. The high-risk areas were primarily distributed in the high-elevation areas along the eastern edge of the Huangshui Basin.
The development characteristics and mechanisms of the Xigou debris flow in the Three Gorges Reservoir Region
Authors: Dai Zhenwei , Zhang Anle , Wang Shufeng , Fu Xiaolin , Yang Longwei , Jiang Xiannian , Wang Heng
Published: January 2023

Debris flow is a common geological hazard in mountainous areas of China, often causing secondary disasters and seriously threatening residents and infrastructure. This paper uses the Xigou debris flow in the Three Gorges Reservoir Region (TGRR) as an example case study, the development characteristics and initiation pattern of which were analyzed based on field investigation. The disaster dynamics software DAN-W was then used to simulate the entire initiation-movement-accumulation process of the debris flow and conduct the debris flow dynamics analysis. The paper also simulated and predicted the movements of landslides in the formation area of a debris flow after its initiation. The results show that the movement duration of the Xigou debris flow was approximately 40 s, the maximum velocity was 37.1 m/s, the maximum thickness of the accumulation was 18.7 m, and the farthest movement distance was 930 m, which are consistent with the field investigation. When the volumes of landslide transformed into a new source material of debris flow are 5 × 104, 10 × 104, 15 × 104, 20 × 104, and 26 × 104 m3, the movement distances of the debris flows are 250, 280, 300, 340, and 375 m, respectively. When the volume of the source material exceeds 20 × 104 m3, debris flow movement can seriously impact the residential houses at the entrance of the gully. This paper can provide a scientific basis for the prevention and mitigation of the Xigou debris flow.
Recycling fertilizers from human excreta exhibit high nitrogen fertilizer value and result in low uptake of pharmaceutical compounds
Authors: Häfner Franziska , Monzon Diaz Oscar Rodrigo , Tietjen Sarah , Schröder Corinna , Krause Ariane
Published: January 2023

Recycling nutrients is essential for closing nutrient loops within a circular economy. Using locally available resources such as human excreta to produce bio-based recycling fertilizers can substitute mineral fertilizers and thereby promote environmentally friendly food production. To better understand the fertilizer potential and nitrogen value of human excreta, three novel and safe recycling products were evaluated in a field experiment. Two nitrified urine fertilizers (NUFs) and one fecal compost were applied alone or in combination, and compared against the commercial organic fertilizer vinasse. In addition, the uptake of pharmaceuticals was assessed for treatments with compost application. White cabbage (Brassica oleracea var. capitata f. alba) was cultivated in plots in three different soil types (sand, loam or silt) treated with the fertilizers according to plant needs and mineral soil nitrogen content. The two NUFs resulted in marketable yields similar to those of vinasse in all soil types. Combining fecal compost with a NUF led to increased marketable yield compared to compost alone. The highest yield was recorded from the sandy soil, where vinasse and NUF treatments led to comparable yields, as expected in organic productions systems (up to 72 t ha−1). The cabbage yield and total aboveground fresh biomass followed the following trend in all soils: NUFs ∼ vinasse ≥ compost + NUF ≥ compost. Nitrogen uptake in the cabbage heads and total biomass was significantly higher in sand (69.5–144 kg ha−1) than loam (71.4–95.8 kg ha−1). All compost treatments alleviated the effect of soil type and resulted in comparable nitrogen uptake and yield in all soil types. Plant uptake of pharmaceuticals (Carbamazepin) was higher in sand than in loam, and concentration in the edible part was lower than in the outer leaves. In conclusion, NUF alone appears to be a promising successful fertilizer substitute in horticultural food production. The combined application of NUF and compost led to slightly lower crop yields, but may increase soil carbon content in the long term, promoting climate-friendly food production.
Long-term outcomes of infantile spasms in children treated with ketogenic diet therapy in combination with anti-seizure medications in a resource-limited region
Authors: Liao Jianxiang , Hu Zhanqi , Lin Sufang , Lu Xinguo , Wen Jialun , Duan Jing , Zou Dongfang , Zou Huafang , Yu Mei , Liu Liqin , Qiao Xiaoying , Ye Yuanzhen
Published: January 2023

Objective

Despite numerous guidelines, the overall outcome of infantile spasms is poor, with only a small number of patients being able to attend school. The purpose of this study was to investigate long-term outcomes. Patients had poor access to the recommended first-line anti-seizure medications (ASMs), such as hormones (corticotropin or prednisolone/prednisone) and vigabatrin, and their alternative treatment was other ASMs and a ketogenic diet.

Methods

Patients suffering from infantile spasms who had at least 2 years of medical records in the electronic medical record system between January 2014 and August 2022 were included in this study. Patient information was retrospectively reviewed. All patients had received ketogenic diet therapy (mainly classical ketogenic diet therapy). The ketogenic diet therapy was combined with ASMs not used as first-line therapies. The primary endpoint outcome measure was the number of patients with seizure freedom. The secondary measures included the duration of ketogenic diet therapy, choice of ASMs, and patient development at the last visit.

Results

A total of 177 patients with infantile spasms were included, and 152 (86%) of them had seizure freedom. The median duration from the first to the last hospital visit was 53.27 months, and the number of visits was 47.00. The median age at the initial hospital visit was 8.00 months, and the median age at initiation of the ketogenic diet was 17.73 months. At the last visit, the proportions of patients with neurodevelopmental delay, developmental epileptic encephalopathy, drug-resistant epilepsy, and generalized seizures increased significantly. The frequently used ASMs were topiramate, valproic acid, levetiracetam, nitrazepam, and vitamin B6 injection, while the recommended first-line drugs corticotropin and vigabatrin were rarely selected. The study duration of 9.5 years was divided into three periods but the prescription of ASMs did not change significantly between these periods.

Conclusions

Although the seizure freedom rate was high with ketogenic diet therapy combined with non-standard ASMs, the patients had a significant neurodevelopmental delay at the last visit, which was, however, similar to that of standard treatment. To improve the outcomes of infantile spasms, multicenter clinical trials of the ketogenic diet as a first-line treatment in combination with non-standard ASMs are needed.
Are there sustained psychological impacts in women diagnosed with in-situ or early invasive breast cancers?
Authors: Braun Bettina , Tio Joke , Krause-Bergmann Barbara , Hense Hans-Werner
Published: January 2023

Purpose

The detection of a ductal carcinoma in-situ (DCIS) or an early invasive breast cancer (EIBC), particularly by population-wide mammography-screening-programs, is controversial as an unknown proportion of these cases may be due to overdiagnosis. We investigated whether women with such potentially overdiagnosed breast cancers suffer from sustained adverse psycho-social consequences.

Methods

Standardized questionnaires were mailed to 900 survivors, diagnosed with either DCIS or EIBC, requesting self-reports on quality of life using EORTC Quality of Life Questionnaire C-30. Levels of anxiety and depression were assessed using the HADS questionnaires. Item score values in the study group were compared to reference data obtained from normative studies in the German female reference population.

Results

The 577 women who returned completed questionnaires had a mean age of 65.1 years, 387 (67%) had been diagnosed by mammography screening. Median time since diagnosis was 5.9 years. There were no substantial differences between the study sample and the reference population for most of the items. While most score values were even slightly more favorable in the study group, the scores for cognitive function were moderately lower, especially among younger patients. Score values for anxiety were generally higher among younger women (50 to 59 years) from the study group, while depression scores were lower irrespective of age.

Conclusions

This study indicates that the diagnosis of DCIS or EIBC, which is predominantly a result of screening, does not seem to induce sustained, adverse psychological impacts in affected women when compared with the respective general female population. Only anxiety levels remained elevated among younger women.
Positive regulators of T cell functions as predictors of prognosis and microenvironment characteristics of low-grade gliomas
Authors: Li Yang , Feng Yabo , Luo Fushu , Peng Gang , Li Yueran
Published: January 2023

Background

Low-grade gliomas (LGG) are one of the most prevalent types of brain cancers. The efficacy of immunotherapy in LGG is limited compared to other cancers. Immunosuppression in the tumor microenvironment (TME) of LGG is one of the main reasons for the low efficacy of immunotherapy. Recent studies have identified 33 positive regulators of T cell functions (TPRs) that play a critical role in promoting the proliferation, activity, and functions of multiple immunocytes. However, their role in the TME of LGG has not been investigated. This study aimed to construct a risk model based on these TPRs and to detect the significance of immunotypes in predicting LGG prognosis and immunotherapy efficacy.

Methods

A total of 688 LGGs and 202 normal brain tissues were extracted from The Cancer Genome Atlas (TCGA), Chinese Glioma Genome Atlas (CGGA), and Genotype-Tissue Expression (GTEx) databases. The NMF R package was used to identify TRP-related subtypes. The TPR prognostic model was established using the least absolute shrinkage and selection operator (LASSO) algorithm to predict the overall survival of LGG samples.

Results

The Subtype 2 patients had worse survival outcomes, suppressed immune function, and higher immune cell infiltration. A risk regression model consisting of 14 TPRs was established, and its performance was validated in CGGA325 cohorts. The low-risk group exhibited better overall survival, immune microenvironment, and immunotherapy response, as determined via the TIDE algorithm, indicating that increasing the level of immune infiltration can effectively improve the response to immunotherapy in the low-risk group. The risk score was determined to be an independent hazard factor (p<0.001) although other clinical features (age, sex, grade, IDH status, 1p19q codel status, MGMT status, and accepted radiotherapy) were considered. Lastly, high-risk groups in both cohorts revealed optimal drug responses to rapamycin, paclitaxel, JW-7-52-1, and bortezomib.

Conclusions

Our study identified two distinct TPR subtypes and built a TPR signature to elucidate the characteristics of T cell proliferation in LGG and its association with immune status and prognosis. These findings shed light on possible immunotherapeutic strategies for LGGs.
Fish mortality associated to volcanic eruptions in the Canary Islands
Authors: Caballero María José , Perez-Torrado Francisco José , Velázquez-Wallraf Alicia , Betancor Mónica Beatriz , Fernández Antonio , Castro-Alonso Ayoze
Published: January 2023

The Canary Islands are an active volcanic archipelago. In the last decade, volcanic activity has occurred twice on the youngest and western most islands: El Hierro (submarine eruption) in 2011-12, and La Palma (subaerial eruption) in 2021. 70 fish specimens of different species from El Hierro volcano and 14 from La Palma were necropsied. A notable high and early mortality of fishes was registered during the submarine eruption of El Hierro. In most of them, generalized congestion in the gills, liver, spleen, kidney, heart and rete mirabile of the swim bladder was observed. Some specimens also presented exophthalmia, gastric eversion, ocular haemorrhages, over-inflation of swim bladder and gas bubbles mainly on the skin and cornea. The eruption of La Palma volcano was characterized by the emission of large amounts of lava flows and pyroclastic deposits (mainly ash in size) both, on land and sea. Lava flowed on land and eventually reached the sea, forming lava deltas in the coastline of La Palma. This event was also associated with an increase in fish mortality in locations near to the lava deltas. Fishes presented ash particles in opercula/oral cavities and gills. In addition, several fishes presented an intense intestinal impaction composed of volcanic material including ashes and hyaloclastites fragments. To our best knowledge this study describes, for the first time, pathological findings in dead fishes associated with two different styles of recent volcanic eruptions in the Canary Islands.
A bio-sustainable approach for reducing Eucalyptus tree-caused agricultural ecosystem hazards employing Trichoderma bio-sustained spores and mycorrhizal networks
Authors: Kabir Md. Golam , Wang Yonglong , Abuhena Md. , Azim Md. Faisal , Al-Rashid Jubair , Rasul Noorain Munim , Mandal Dipa , Maitra Pulak
Published: January 2023

The presence of the exotic Eucalyptus tree in crop-growing soil and the accumulation of its undecomposed leaves is a significant ecological hazard. The waxy coating on the leaves and the phenolic compounds takes a long time to break down under normal conditions. It is necessary to explore various fungi that can degrade these leaves for an eco-friendly solution to this problem. In this study, spores of nine native Trichoderma strains were produced on wheat agar using a lactic acid-induced sporulation strategy (LAISS). Trichoderma biosustained spores and Serendipita indica (SI) spores were applied to a rice field with accumulated Eucalyptus leaves under continuous ponding (CP) and alternate flooding and wetting conditions (AFW). Among the strains, TI04 (Trichoderma viride) and TI15 (Trichoderma citrinoviride) showed faster (5 days) and massive sporulation (1.06–1.38 × 1011 CFU/g) in LAISS. In vitro, TI04 and TI15 biosustained on Eucalyptus leaves and improved rice seedling growth and SI infection under greenhouse conditions. In the rice-field experiment, Trichoderma-treatment had a threefold yield (percentage) increase from control, with TI04 (CP) increasing the yield by 30.79, TI04 (AFW) by 29.45, TI15 (CP) by 32.72, and TI15 (AFW) rising by 31.91. Remarkably, unfilled grain yield significantly decreased in all the Trichoderma treatments. Under AFW conditions, TI04 and TI15 showed a higher pH increase. Furthermore, TI04 and TI15 under AFW had higher water productivity (t ha−1 cm−1) of 0.0763 and 0.0791, respectively, and the highest rates (percentage) of SI colonization of 86.36 and 83.16, respectively. According to the findings, LAISS-produced Trichoderma spores can be applied to break down persistent wastes and restore agricultural ecosystems through increased mycorrhizae networking.
Population dynamics of Brachionus calyciflorus driven by the associated natural bacterioplankton
Authors: Zhang Yongzhi , Feng Sen , Zhu Lingyun , Li Meng , Xiang Xianling
Published: January 2023

Zooplankton provides bacteria with a complex microhabitat richen in organic and inorganic nutrients, and the bacteria community also changes the physiochemical conditions for zooplankton, where the symbiotic relationship between them plays an important role in the nutrient cycle. However, there are few studies on the effect of associated bacteria on the population dynamics of rotifers. In order to make clear their relationships, we reconstructed the associated bacterial community in Brachionus calyciflorus culture, and examined the life history and population growth parameters, and analyzed the diversity and community composition of the associated bacteria at different growth stages of B. calyciflorus. The results showed that the addition of bacteria from natural water can promote the population growth and asexual reproduction of B. calyciflorus, but has no significant effect on sexual reproduction, exhibited by the improvement of its life expectancy at hatching, net reproduction rates and intrinsic growth rate, no significant effects on the generation time and mixis ratio of offspring. It was found that the B. calyciflorus-associated bacterial community was mainly composed of Proteobacteria, Bacteroidota, Actinobacteriota, Cyanobacteria and Firmicutes. Through correlation network analysis, the members of Burkholderiales, Pseudomonadales, Micrococcales, Caulobacterales and Bifidobacteriales were the keystone taxa of B. calyciflorus-associated bacteria. In addition, the relative abundance of some specific bacteria strains increased as the population density of B. calyciflorus increased, such as Hydrogenophaga, Acidovorax, Flavobacterium, Rheinheimera, Novosphingobium and Limnobacter, and their relative abundance increased obviously during the slow and exponential phases of population growth. Meanwhile, the relative abundance of adverse taxa (such as Elizabethkingia and Rickettsiales) decreased significantly with the increase in rotifer population density. In conclusion, the closely associated bacteria are not sufficient for the best growth of B. calyciflorus, and external bacterioplankton is necessary. Furthermore, the function of keystone and rare taxa is necessary for further exploration. The investigation of the symbiotic relationship between zooplankton-associated bacterial and bacterioplankton communities will contribute to monitoring their roles in freshwater ecosystems, and regulate the population dynamics of the micro-food web.
The significance of the crosstalk between ubiquitination or deubiquitination and ncRNAs in non-small cell lung cancer
Authors: Sun Yiyang , He Ping , Li Li , Ding Xue
Published: January 2023

Lung cancer (LC) remains the leading cause of cancer-related deaths worldwide, with extremely high morbidity and mortality rates. Non-small cell lung cancer (NSCLC) is the most critical type of LC. It seriously threatens the life and health of patients because of its early metastasis, late clinical symptoms, limited early screening methods, and poor treatment outcomes. Non-coding RNAs (ncRNAs), including microRNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs), participate in cell proliferation, metastasis, and chemoresistance. Several previous studies have proven that ncRNAs are vital regulators of tumorigenesis. Ubiquitination plays the most crucial role in protein post-translational modification (PTM). Deubiquitination and ubiquitination form a homeostasis. In summary, ubiquitination and deubiquitination play essential roles in mediating the degradation or overexpression of a range of crucial proteins in various cancers. A growing number of researchers have found that interactions between ncRNAs and ubiquitination (or deubiquitination) play a crucial role in NSCLC. This review presents several typical examples of the important effects of ncRNAs and ubiquitination (or deubiquitination) in NSCLC, aiming to provide more creative ideas for exploring the diagnosis and treatment of NSCLC.
Impact of clinicopathological factors on extended endocrine therapy decision making in estrogen receptor–positive breast cancer
Authors: Chen Weilin , Wu Jiayi , Zhu Yifei , Huang Jiahui , Chen Xiaosong , Huang Ou , He Jianrong , Li Yafen , Chen Weiguo , Shen Kunwei , Zhu Li
Published: January 2023

Purpose

In our study, we aim to analyze the impact of clinicopathological factors on the recommendation of extended endocrine therapy (EET) in patients with ER+ breast cancer and to retrospectively validate the value of CTS5 in EET decision making.

Patients and methods

The retrospective analysis was performed in patients with ER+ breast cancer who have finished 4.5–5 years of adjuvant endocrine therapy and undergone MDT discussion from October 2017 to November 2019. Multivariate logistic regression was used to identify the independent factors for treatment recommendation. CTS5 was calculated for retrospective validation of the EET decision making.

Results

Two hundred thirty-five patients were received; 4.5–5 years of adjuvant endocrine therapy were included in the study. Multivariate analysis suggested that age (OR 0.460, 95% CI 0.219–0.965, p = 0.04), pN (OR 39.350, 95% CI 9.831–157.341, P < 0.001), and receipt of chemotherapy (OR 3.478, 95% CI 1.336–9.055, p = 0.011) were independent predictors for the recommendation of EET. In the previously selective estrogen receptor modulator (SERM)–treated subgroup, pN and receipt of chemotherapy were independent predictors for the recommendation of EET. In the previously AI-treated subgroup, age, pN, and receipt of chemotherapy were independent predictors. Adverse events did not affect the recommendation in patients previously treated with adjuvant endocrine treatment nor in the previously SERM or AI-treated subgroups. CTS5 (OR 21.887, 95% CI 2.846–168.309, p = 0.003) remained an independent predictor for the recommendation of EET.

Conclusions

Our study indicated that age, lymph nodal status, and receipt of chemotherapy were independent predictors for the recommendation of EET. The application of the CTS5 on EET decision making might be valuable among ER+ breast cancer patients.
Identification of MTHFD2 as a prognostic biomarker and ferroptosis regulator in triple-negative breast cancer
Authors: Zhang Hao , Zhu Shuangli , Zhou Haiting , Li Rui , Xia Xiaohui , Xiong Huihua
Published: January 2023

Background

Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a mitochondrial bifunctional enzyme encoded in the nucleus. It plays a significant role in the regulation of glucose, nucleic acid, and folate metabolism, and maintains redox balance in the cells. The present study aimed at elucidating the potential function and mechanisms of MTHFD2 and explored the correlation between ferroptosis and MTHFD2 in triple-negative breast cancer.

Methods

MTHFD2 expression, survival analysis, and clinical correlation were performed using data from various online databases including TCGA, GEO, HPA, GTEX, Kaplan–Meier Plotter, PrognoScan, and UALCAN databases. Genomic alterations and CNV analysis were performed using the cBioPortal and GSCA databases. Potential functions and mechanisms were explored by enrichment analysis. The tumor microenvironment was identified by the TIMER database. In vitro, RT-qPCR and western blot assays were utilized to identify the MTHFD2 expression and the knockdown effects in breast cancer. CCK8, cell wound healing, transwell, and flow cytometry assays were used to identify the potential function of MTHFD2 in TNBC cells. MDA, GSH detection, and flow cytometry assays were performed to identify ferroptosis. Western blot assays were performed to measure the protein expression of all target genes.

Results

MTHFD2 expression levels were up-regulated in the majority of cancers and particularly in TNBC, in which higher expression levels indicated a poorer prognosis. Enrichment analyses showed that MTHFD2 is involved in various tumor-related biological processes. MTHFD2 expression was found to strongly correlate with multiple immune cell infiltration. In vitro, the knockdown of MTHFD2 suppresses the proliferation, apoptosis, migration, and invasion in TNBC cells. In addition, the MTHFD2 knockdown significantly enhanced intracellular ROS and lipid peroxidation and decreased intracellular GSH. The expressions of SLC7A11, GPX4, and NRF2 were down-regulated by the MTHFD2 knockdown.

Conclusion

MTHFD2 could be a crucial molecular biomarker for predicting patient prognosis and a novel therapeutic target in TNBC. In addition, MTHFD2 is a potential ferroptosis regulatory gene in TNBC.
Aldosterone defects in infants and young children with hyperkalemia: A single center retrospective study
Authors: Liu Xu , Xie Yanshu , Tang Jing , Zhong Jingzi , Zeng Dan , Lan Dan
Published: January 2023

Introduction

Hyperkalemia is a rare but severe condition in young children and usually discovered as a result of hemolysis of the blood samples taken. However, patients with defects in either aldosterone biosynthesis or function can also present with hyperkalemia- as well hyponatremia-associated, and metabolic acidosis. It is a challenge to make an accurate diagnosis of these clinical conditions. We conducted this study to investigate the clinical and genetic features of aldosterone signaling defects associated hyperkalemia in young children.

Method

A retrospective review was conducted at the pediatric department of the First Affiliated Hospital of Guangxi Medical University from 2012 to 2022.

Results

47 patients with hyperkalemia were enrolled, of which 80.9% (n = 38) were diagnosed with primary hypoaldosteronism, including congenital adrenal hyperplasia due to 21-hydroxylase deficiency (n = 32), isolated hypoaldosteronism (n = 1) due to CYP11B2 gene mutation and Xp21 contiguous gene deletion syndrome (n = 1). Additionally, 4 patients were clinically-diagnosed with primary adrenal insufficiency. Nine patients were confirmed with aldosterone resistance, of which one child was diagnosed with pseudohypoaldosteronism (PHA) type 1 with a mutation in the NR3C2 gene and 3 children were identified with PHA type 2 due to novel mutations in either the CUL3 or KLHL3 genes. Five patients had PHA type 3 because of pathologies of either the urinary or intestinal tracts.

Conclusions

The etiologies of infants with hyperkalemia associated with aldosterone defects were mostly due to primary hypoaldosteronism. An elevated plasma aldosterone level may be a useful biomarker for the diagnosis an aldosterone functional defect in patients presented with hyperkalemia. However, a normal plasma aldosterone level does rule out an aldosterone defect in either its biosynthesis or function, especially in young infants. Molecular genetic analyses can greatly help to clarify the complexity of disorders and can be used to confirm the diagnosis.
Role of adrenergic receptors in shock
Authors: Geevarghese Mathew , Patel Krishna , Gulati Anil , Ranjan Amaresh K.
Published: January 2023

Shock is a severe, life-threatening medical condition with a high mortality rate worldwide. All four major categories of shock (along with their various subtypes)—hypovolemic, distributive, cardiogenic, and obstructive, involve a dramatic mismatch between oxygen supply and demand, and share standard features of decreased cardiac output, reduced blood pressure, and overall hypoperfusion. Immediate and appropriate intervention is required regardless of shock type, as a delay can result in cellular dysfunction, irreversible multiple organ failure, and death. Studies have shown that dysfunction and downregulation of adrenergic receptors (ARs) are often implicated in these shock conditions; for example, their density is shown to be decreased in hypovolemic and cardiogenic shock, while their reduced signaling in the brain and vasculature decrease blood perfusion and oxygen supply. There are two main categories of ARs, α, and β, each with its subtypes and distributions. Our group has demonstrated that a dose of .02 mg/kg body wt of centhaquine (CQ) specifically activates α2B ARs on venous circulation along with the central α2A ARs after hypovolemic/hemorrhagic shock. Activating these receptors by CQ increases cardiac output (CO) and reduces systemic vascular resistance (SVR), with a net increase in blood pressure and tissue perfusion. The clinical trials of CQ conducted by Pharmazz Inc. in India have demonstrated significantly improved survival in shock patients. CQ improved blood pressure and shock index, indicating better blood circulation, and reduced lactate levels in the blood compared to in-use standard resuscitative agents. After successful clinical trials, CQ is being marketed as a drug (Lyfaquin®) for hypovolemic/hemorrhagic shock in India, and United States FDA has approved the phase III IND application. It is anticipated that the phase III trial in the United States will begin in 2023. Thus, we have demonstrated that α2 ARs could be suitable targets for treating or managing hypovolemic/hemorrhagic shock. Further understanding of ARs in shock would help find new potential pharmacological targets.
Endocytic trafficking induces lateral root founder cell specification in Arabidopsis thaliana in a process distinct from the auxin-induced pathway
Authors: Morales-Herrera Stefanía , Rubilar-Hernández Carlos , Pérez-Henríquez Patricio , Norambuena Lorena
Published: January 2023

Plants can modify their body structure, such as their root architecture, post-embryonically. For example, Arabidopsis thaliana can develop lateral roots as part of an endogenous program or in response to biotic and abiotic stimuli. Root pericycle cells are specified to become lateral root founder cells, initiating lateral root organogenesis. We used the endocytic trafficking inducer Sortin2 to examine the role of endomembrane trafficking in lateral root founder cell specification. Our results indicate that Sortin2 stimulation turns on a de novo program of lateral root primordium formation that is distinct from the endogenous program driven by auxin. In this distinctive mechanism, extracellular calcium uptake and endocytic trafficking toward the vacuole are required for lateral root founder cell specification upstream of the auxin module led by AUX/IAA28. The auxin-dependent TIR1/AFB F-boxes and auxin polar transport are dispensable for the endocytic trafficking–dependent lateral root founder cell specification; however, a different set of F-box proteins and a functional SCF complex are required. The endocytic trafficking could constitute a convenient strategy for organogenesis in response to environmental conditions.
Genetic dissection of marker trait associations for grain micro-nutrients and thousand grain weight under heat and drought stress conditions in wheat
Authors: Devate Narayana Bhat , Krishna Hari , Mishra Chandra Nath , Manjunath Karthik Kumar , Sunilkumar V. P. , Chauhan Divya , Singh Shweta , Sinha Nivedita , Jain Neelu , Singh Gyanendra Pratap , Singh Pradeep Kumar
Published: January 2023

Introduction

Wheat is grown and consumed worldwide, making it an important staple food crop for both its calorific and nutritional content. In places where wheat is used as a staple food, suboptimal micronutrient content levels, especially of grain iron (Fe) and zinc (Zn), can lead to malnutrition. Grain nutrient content is influenced by abiotic stresses, such as drought and heat stress. The best method for addressing micronutrient deficiencies is the biofortification of food crops. The prerequisites for marker-assisted varietal development are the identification of the genomic region responsible for high grain iron and zinc contents and an understanding of their genetics.

Methods

A total of 193 diverse wheat genotypes were evaluated under drought and heat stress conditions across the years at the Indian Agricultural Research Institute (IARI), New Delhi, under timely sown irrigated (IR), restricted irrigated (RI) and late sown (LS) conditions. Grain iron content (GFeC) and grain zinc content (GZnC) were estimated from both the control and treatment groups. Genotyping of all the lines under study was carried out with the single nucleotide polymorphisms (SNPs) from Breeder’s 35K Axiom Array.

Result and Discussion

Three subgroups were observed in the association panel based on both principal component analysis (PCA) and dendrogram analysis. A large whole-genome linkage disequilibrium (LD) block size of 3.49 Mb was observed. A genome-wide association study identified 16 unique stringent marker trait associations for GFeC, GZnC, and 1000-grain weight (TGW). In silico analysis demonstrated the presence of 28 potential candidate genes in the flanking region of 16 linked SNPs, such as synaptotagmin-like mitochondrial-lipid-binding domain, HAUS augmin-like complex, di-copper center-containing domain, protein kinase, chaperonin Cpn60, zinc finger, NUDIX hydrolase, etc. Expression levels of these genes in vegetative tissues and grain were also found. Utilization of identified markers in marker-assisted breeding may lead to the rapid development of biofortified wheat genotypes to combat malnutrition.
Children’s social emotional competence in Pakistan and Sweden: Factor structure and measurement invariance of the Social Competence Scale (teacher edition)
Authors: Thomas Sarah , Kågström Anna , Eichas Kyle , Inam Ayesha , Ferrer-Wreder Laura , Eninger Lilianne
Published: January 2023

Introduction

Social emotional competence is fundamental to the positive development of children and youth. Accurately understanding and assessing children’s social emotional competencies, using psychometrically sound instruments, are essential to global efforts to support children’s social emotional learning, academic achievements, and health. This study examined the psychometric properties of a teacher-reported measure of young children’s social emotional competence, the Social Competence Scale – Teacher edition (SCS-T), in two samples of children growing up with varied economic resources/conditions, cultural norms, and educational experiences, namely Pakistan (N = 396) and Sweden (N = 309).

Methods

Participants were aged 4–6 years old. The study design was cross-sectional.

Results and Discussion

Using structural equation modelling, bi-factor confirmatory factor analysis models implying shared variance, among all items and domain-specific shared variance, among the prosocial items, emotion regulation items, and academic skills items resulted in good fitting models in each respective sample. Invariance testing across samples revealed a subset of items from each factor structure with partial scalar invariance, whereby five items had equal thresholds and could be comparable across the two samples. Thus, results provided partial support for hypotheses 1, 2, and 3, in that the posited three factor model (H1) was not clearly supported and a bi-factor model evidenced the best fit, among tested models, for both samples. Further, partial scalar invariance (H3) was found for five items out of 25 items, concerning social competence and academic skills. In regards, to the posited research question, the results of Z-tests showed significant (p < 0.001) latent mean differences between the samples. Compared to the Swedish sample, the Pakistani sample was 1.80 units lower on social competence (z = −6.41, p < 0.001) and 1.86 units lower on academic skills (z = −7.87, p < 0.001). The implications of these findings in light of efforts to promote positive child development in diverse parts of the world are considered.
Parenting motives: Validation of the Italian version of the parental care and tenderness scale
Authors: Castelli Luigi , Garau Tania , Carraro Luciana
Published: January 2023

Relevant individual differences can be observed in relation to parenting motives. The Parental Care and Tenderness (PCAT) scale is an important tool aimed at assessing them. We here investigated the psychometric properties of an Italian version of the scale (N = 946). The scale had a very high reliability and its internal structure closely reproduced the one obtained in different cultural contexts. Two major subscales, namely nurturance and protection, could be identified. In addition, we explored the validity of the scale in relation to a novel domain. Individual differences in parenting motivations, and more specifically those related to nurturance, emerged to be associated with a spontaneous behavioral tendency to approach children. Scores in the PCAT, and more specifically scores in the protection subscale, were also associated with a more conservative political orientation. The present work contributes to the growing literature about the key role of parenting motives in affecting social behaviors.
How does the perceived green human resource management impact employee’s green innovative behavior? —From the perspective of theory of planned behavior
Authors: Song Dian , Bai Yan , Wu Hainan , Wang Xiaoyuan
Published: January 2023

Employees’ green innovative behavior encouraged by enterprises plays an important role in the enterprise sustainable development. The study explores the impact of perceived green human resource management on employees’ innovative behavior. Drawing upon the planned behavior theory, this study examines how perceived green human resource management impact employees’ green innovation behavior. Through three-stage questionnaire survey, 207 samples are obtained and hierarchical regression is employed to test the hypothesis., Data analysis results show that perceived green human resource management has a directly positive effect on employees’ green innovative behavior. Green behavior intention, self-efficacy of environmental protection behavior, and identity with the company’s green environmental protection system are the mediators between perceived green human resource management and employees’ green innovative behavior. Meanwhile, the results demonstrate that there is a chain mediating relationship among these variables. In addition, green supply chain management moderates the relationship between the identity of a green environmental protection system and employees’ green innovative behavior. These conclusions transcend the macro perspective and open the black box between green human resource management and enterprise performance. Enterprise should take a holistic view to play the role of green human resource management and supply chain management in the implementation of environmental strategy.
The Tapping-PROMS: A test for the assessment of sensorimotor rhythmic abilities
Authors: Georgi Markus , Gingras Bruno , Zentner Marcel
Published: January 2023

Sensorimotor synchronization is a longstanding paradigm in the analysis of isochronous beat tapping. Assessing the finger tapping of complex rhythmic patterns is far less explored and considerably more complex to analyze. Hence, whereas several instruments to assess tempo or beat tapping ability exist, there is at present a shortage of paradigms and tools for the assessment of the ability to tap to complex rhythmic patterns. To redress this limitation, we developed a standardized rhythm tapping test comprising test items of different complexity. The items were taken from the rhythm and tempo subtests of the Profile of Music Perception Skills (PROMS), and administered as tapping items to 40 participants (20 women). Overall, results showed satisfactory psychometric properties for internal consistency and test–retest reliability. Convergent, discriminant, and criterion validity correlations fell in line with expectations. Specifically, performance in rhythm tapping was correlated more strongly with performance in rhythm perception than in tempo perception, whereas performance in tempo tapping was more strongly correlated with performance in tempo than rhythm perception. Both tapping tasks were only marginally correlated with non-temporal perception tasks. In combination, the tapping tasks explained variance in external indicators of musical proficiency above and beyond the perceptual PROMS tasks. This tool allows for the assessment of complex rhythmic tapping skills in about 15 min, thus providing a useful addition to existing music aptitude batteries.
Mind the gap: Exploring differences in suicide literacy between cybersuicide and offline suicide
Authors: Li Ang , Jiao Dongdong
Published: January 2023

Introduction

The highly public nature of cybersuicide contradicts long-held beliefs of offline suicide, which may cause differences in the way people perceive and respond to both of them. However, knowledge of whether and how suicide literacy differs between cybersuicide and offline suicide is limited.

Methods

By analyzing social media data, this paper focused on livestreamed suicide and aimed to compare suicide literacy between cybersuicide and offline suicide on three aspects, including false knowledge structure, extent of association with stigma, and linguistic expression pattern. 7,236 Sina Weibo posts with relevant keywords were downloaded and analyzed. First, a content analysis was performed by human coders to determine whether each post reflected suicide-related false knowledge and stigma. Second, a text analysis was conducted using the Simplified Chinese version of LIWC software to automatically extract psycholinguistic features from each post. Third, based on selected features, classification models were developed using machine learning techniques to differentiate false knowledge of cybersuicide from that of offline suicide.

Results

Results showed that, first, cybersuicide-related posts generally reflected more false knowledge than offline suicide-related posts (χ12=255.13, p < 0.001). Significant differences were also observed in seven false knowledge types. Second, among posts reflecting false knowledge, cybersuicide-related posts generally carried more stigma than offline suicide-related posts (χ12 = 116.77, p < 0.001). Significant differences were also observed in three false knowledge types. Third, among established classification models, the highest F1 value reached 0.70.

Discussion

The findings provide evidence of differences in suicide literacy between cybersuicide and offline suicide, and indicate the need for public awareness campaigns that specifically target cybersuicide.
Anesthetic management for cytoreductive surgery of pseudomyxoma peritonei with high intra-abdominal pressure: A case report
Authors: Lin Yan-Jun , Lu Liang-Yuan , Tao De-Qiang , Yang Zhan-Min
Published: January 2023

Anesthetic management for patients of pseudomyxoma peritonei (PMP) is challenging. This case report describes a patient of PMP with high intra-abdominal pressure. Intubation was performed in lateral position; the intraabdominal pressure was relieved slowly to prevent significant hemodynamic changes. Additionally, positive pressure ventilation was performed to reduce the risk of re-expansion pulmonary edema. During the operation, transfusion and infusion therapy was performed with target-mediated fluid therapy according to stroke volume variation (SVV) and cardiac index (CI) and blood gas analysis.