bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of Negative Informal Information Before a Change on Performance: A Within-Person Approach
Authors: Jiang Xia , Du Jing , Zhou Jinfan , Cui Yumeng
Published: January 2020

We live in a rapidly changing business environment where change has become the norm for organizations to maintain competitiveness. Although both scholars and practitioners agree that organizational change communication is important to help employees adjust to change, little is known about how negative informal information before the change affects employees’ reaction to the change and occurrence of possible within-person dynamics of resistance intention over time. Based on the construal-level theory, we used SPSS 22, AMOS 20, and HLM 6.0 as tools to explore how negative informal information affects individual performance. We used a multilevel approach to probe within-person processes among 215 MBA students in China. The results show that (1) negative informal information provided before the organizational change is positively related to the resistance intention, (2) resistance intention decreases significantly over time, and (3) negative informal information is negatively related to individual performance during the organizational change. The results from this study extend the literature on informal communication before the change and provide a dynamic perspective on the occurrence of possible within-person dynamics of resistance intention over time.
Regional Influenza Prediction with Sampling Twitter Data and PDE Model
Authors: Wang Yufang , Xu Kuai , Kang Yun , Wang Haiyan , Wang Feng , Avram Adrian
Published: January 2020

The large volume of geotagged Twitter streaming data on flu epidemics provides chances for researchers to explore, model, and predict the trends of flu cases in a timely manner. However, the explosive growth of data from social media makes data sampling a natural choice. In this paper, we develop a method for influenza prediction based on the real-time tweet data from social media, and this method ensures real-time prediction and is applicable to sampling data. Specifically, we first simulate the sampling process of flu tweets, and then develop a specific partial differential equation (PDE) model to characterize and predict the aggregated flu tweet volumes. Our PDE model incorporates the effects of flu spreading, flu recovery, and active human interventions for reducing flu. Our extensive simulation results show that this PDE model can almost eliminate the data reduction effects from the sampling process: It requires lesser historical data but achieves stronger prediction results with a relative accuracy of over 90% on the 1% sampling data. Even for the more aggressive data sampling ratios such as 0.1% and 0.01% sampling, our model is still able to achieve relative accuracies of 85% and 83%, respectively. These promising results highlight the ability of our mechanistic PDE model in predicting temporal–spatial patterns of flu trends even in the scenario of small sampling Twitter data.
Adhering to the 2017 Dutch Physical Activity Guidelines: A Trend over Time 2001–2018
Authors: Duijvestijn Marjolein , van den Berg Saskia W. , Wendel-Vos G. C. Wanda
Published: January 2020

Recently, new physical activity (PA) guidelines were adopted in the Netherlands consisting of two components: (1) addressing duration of moderate and vigorous PA, (2) bone and muscle strengthening activities. The aim of this study is to retrospectively assess the long-term trend in fulfilling the criteria of the new PA guidelines and to gain insight into which activities contribute to changes over time. Data were available for 2001–2018 of a nationally representative sample of approximately 7000 Dutch citizens aged 12 years and over using the Short Questionnaire to Assess Health-enhancing physical activity (SQUASH). Multiple logistic regression analysis was performed by age, sex, and level of education. Overall, a positive trend was found from 39.9% adherence in 2001 to 46.0% in 2018. Adherence levels among adolescents decreased and increased among adults and seniors. Intermediate and higher educated groups showed positive trends over time whereas a stable trend was observed among lower educated. Activities contributing most to changes over time were sports, leisure time walking, and strenuous occupational activities. In the period 2001–2018, though an increasing trend was found, less than half of the population was sufficiently active. Special effort is necessary to reach adolescents, seniors, and lower educated groups in PA promotion programs.
Removal of Hydrogen Sulfide in Septic Tanks for Treating Black Water via an Immobilized Media of Sulfur-Oxidizing Bacteria
Authors: Kang Jeong-Hee , Namgung Hyeong-Gyu , Cho Jeong-Il , Yoo Sung Soo , Lee Bong-Jae , Ji Hyon Wook
Published: January 2020

In South Korea, the installation of septic tanks for treating black water (STBW) is regulated even in sewage treatment areas to prevent the black water deposition in combined sewers. STBWs in which black water is anaerobically decomposed generate high concentrations of hydrogen sulfide (H2S). In this study, an immobilized media of sulfur-oxidizing bacteria (SOB) was used to remove the H2S. SOB media was prepared by using activated sludge collected from a wastewater treatment plant. Prior to field application, an appropriate cultivation period and aeration rate for SOB activation were estimated through a laboratory-scale test. The SOB was activated after a 23-day cultivation period and an aeration rate of 0.25 L-water/L-air/min. Moreover, the maximum H2S removal efficiency was observed at a cultivation period of 43 days and an aeration rate of 0.38 L-water/L-air/min. Then, the SOB media was installed on STBWs of various capacities. The H2S removal efficiency was compared between with and without SOB media. The maximum H2S elimination capacity with SOB media was 12.3 g/m3/h, which was approximately three times higher than without SOB media. Furthermore, the energy efficiency and oxidation rate were also three times higher with SOB, demonstrating the applicability of SOB for H2S removal in STBW.
Six-Step Model of Nature-Based Therapy Process
Authors: Oh Kyung Hee , Shin Won Sop , Khil Tae Gyu , Kim Dong Jun
Published: January 2020

Several studies have confirmed that the natural environment has psychophysiological healing effects. However, few studies have investigated the healing process involved in the effect of the natural environment. To date, no theoretical model on the nature-based therapy process has been clearly established. Thus, the aim of this study was to develop a theoretical model of the nature-based therapy process by analyzing individual empirical data. Research materials were 180 self-reported essays on “Forest Therapy Experiences” submitted to the Korea Forest Service. This study was conducted based on grounded theory. Data were analyzed through open coding. A total of 82 concepts, 21 subcategories, and six categories were derived. Results revealed that the nature-based therapy process contained six categories: Stimulation, acceptance, purification, insight, recharging, and change. When in the natural environment, participants first experienced positive emotional change, followed by cognitive and behavioral changes. Based on these results, a nature-based therapy process model was derived. This study revealed that the nature-based therapy process did not consist of just a single element or step, but involved an integrated way of healing with emotional and cognitive changes. This study is significant in that it derives a theoretical model of the nature-based therapy process with comprehensive mechanisms. Further research is needed to establish more systematic theoretical model.
A Systematic Review of Beef Meat Quantitative Microbial Risk Assessment Models
Authors: Tesson Vincent , Federighi Michel , Cummins Enda , de Oliveira Mota Juliana , Guillou Sandrine , Boué Géraldine
Published: January 2020

Each year in Europe, meat is associated with 2.3 million foodborne illnesses, with a high contribution from beef meat. Many of these illnesses are attributed to pathogenic bacterial contamination and inadequate operations leading to growth and/or insufficient inactivation occurring along the whole farm-to-fork chain. To ensure consumer health, decision-making processes in food safety rely on Quantitative Microbiological Risk Assessment (QMRA) with many applications in recent decades. The present study aims to conduct a critical analysis of beef QMRAs and to identify future challenges. A systematic approach, the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, was used to collate beef QMRA models, identify steps of the farm-to-fork chain considered, and analyze inputs and outputs included as well as modelling methods. A total of 2343 articles were collected and 67 were selected. These studies focused mainly on western countries and considered Escherichia coli (EHEC) and Salmonella spp. pathogens. Future challenges were identified and included the need of whole-chain assessments, centralization of data collection processes, and improvement of model interoperability through harmonization. The present analysis can serve as a source of data and information to inform QMRA framework for beef meat and will help the scientific community and food safety authorities to identify specific monitoring and research needs.
Inflammatory Biomarkers, Microbiome, Depression, and Executive Dysfunction in Alcohol Users
Authors: Rodríguez-Rabassa Mary , López Pablo , Sánchez Raphael , Hernández Cyanela , Rodríguez Cesarly , Rodríguez-Santiago Ronald E. , Orengo Juan C. , Green Vivian , Yamamura Yasuhiro , Rivera-Amill Vanessa
Published: January 2020

Alcohol-related disorders (ARD) are highly prevalent among Latin American-Caribbean countries. Mental disorders are common comorbidities in individuals with ARD. However, the etiology of the association between ARD and mental disorders remains unclear. We examined the association of inflammatory cytokines, microbiome, and other biomakers with measures of depression, social anxiety, and executive functions. We observed a significant increase in cytokine and chemokine expression levels in saliva and plasma in the alcohol group (AG) samples. Also, the salivary bacterial composition in the AG revealed an abundance of Prevotella. Depression symptomatology was markedly higher in the AG, but social anxiety levels were negligible. AG also exhibited executive dysfunctions, which negatively correlated with increased plasma levels of pro-inflammatory cytokines and increased salivary concentrations of Prevotella bacteria. Our study suggests that chronic alcohol use correlates with executive dysfunction, immune system dysregulation, and dysbiosis of the salivary microbiota. Additional studies are needed to understand the role of the microbiome and inflammation in alcohol use and mental comorbidities.
Cognitive-Affective Change Mechanisms in Personalized Normative Feedback via the Articulated Thoughts in Simulated Situations Paradigm
Authors: Hummer Justin F. , Hatch Melissa R. , Davison Gerald C.
Published: January 2020

The research explored explanatory mechanisms of change for a personalized normative feedback (PNF) intervention, through an adapted application of the Articulated Thoughts in Simulated Situation (ATSS) cognitive think-aloud paradigm. A sample of 70 (51% female) U.S. adjudicated students were randomly assigned to one of three conditions: a PNF-ATSS condition, a PNF-Only condition (without ATSS), and an active Control+ATSS condition which received psychoeducation about alcohol use. Students in both the PNF-Only and PNF-ATSS conditions reported significant reductions in their misperceived peer drinking norms and alcohol-related consequences at the 30-day follow-up, relative to students in the control condition. Participants in the PNF-ATSS condition drank significantly fewer drinks per week at follow-up than participants in the PNF-Only condition, but not less than participants in the control condition. Significant indirect effects were found for the ATSS codes of participants’ neutrality and believability toward PNF content. This study presents a proof of concept for an adapted ATSS think-aloud methodology as a clinical science intervention tool to specify the cognitive-affective processes of change linked to complex intervention for particular problems, persons, and contexts.
Parental Attachment, Adult-Child Romantic Attachment, and Marital Satisfaction: An Examination of Cultural Context in Taiwanese and Thai Heterosexual Couples
Authors: Huang Ching-Yu , Sirikantraporn Skultip , Pichayayothin Nipat Bock , Turner-Cobb Julie M.
Published: January 2020

Relationship dynamics between married couples can differ considerably, with varying impacts on relationship satisfaction. However, very limited research attention has been paid to how intergenerational attachment, relating to an individual’s perception of his/her own and that of his/her parents’ attachment, can affect marital dynamics within different cultural contexts. The current study examined associations between married heterosexual couples’ romantic attachment, perception of parental attachment, and marital satisfaction in 100 Thai couples (M age = 45.59 years, SD = 10.86) and 73 Taiwanese couples (M age = 39.55 years, SD = 9.13). Results revealed that romantic attachment anxiety was negatively associated with marital satisfaction in the Taiwanese couples; in the Thai couples, neither romantic attachment anxiety nor avoidance was associated with marital satisfaction. Husbands reported higher romantic attachment anxiety than their wives in Taiwan, but this was not observed in the Thai couples. Taiwanese wives reported higher scores on their perceived parental attachment avoidance than did their husbands; whereas the reverse trend was observed in the Thai couples. These findings highlight the need to consider intergenerational aspects of attachment in cultural contexts, and they have important implications for practitioners working with couples from Asian cultural backgrounds.
Low-Cost Robotic Guide Based on a Motor Imagery Brain–Computer Interface for Arm Assisted Rehabilitation
Authors: Quiles Eduardo , Suay Ferran , Candela Gemma , Chio Nayibe , Jiménez Manuel , Álvarez-Kurogi Leandro
Published: January 2020

Motor imagery has been suggested as an efficient alternative to improve the rehabilitation process of affected limbs. In this study, a low-cost robotic guide is implemented so that linear position can be controlled via the user’s motor imagination of movement intention. The patient can use this device to move the arm attached to the guide according to their own intentions. The first objective of this study was to check the feasibility and safety of the designed robotic guide controlled via a motor imagery (MI)-based brain–computer interface (MI-BCI) in healthy individuals, with the ultimate aim to apply it to rehabilitation patients. The second objective was to determine which are the most convenient MI strategies to control the different assisted rehabilitation arm movements. The results of this study show a better performance when the BCI task is controlled with an action–action MI strategy versus an action–relaxation one. No statistically significant difference was found between the two action–action MI strategies.
Spatiotemporal Analysis of the Population Risk of Congenital Microcephaly in Pernambuco State, Brazil
Authors: Alexander Neal D. E. , Souza Wayner V. , Rodrigues Laura C. , Braga Cynthia , Sá André , Albuquerque Bezerra Luciana Caroline , Turchi Martelli Celina Maria
Published: January 2020

Since an outbreak in Brazil, which started in 2015, Zika has been recognized as an important cause of microcephaly. The highest burden of this outbreak was in northeast Brazil, including the state of Pernambuco. The prevalence of congenital microcephaly in Pernambuco state was estimated from the RESP (Registro de Eventos em Saúde Pública) surveillance system, from August 2015 to August 2016 inclusive. The denominators were estimated at the municipality level from official demographic data. Microcephaly was defined as a neonatal head circumference below the 3rd percentile of the Intergrowth standards. Smoothed maps of the prevalence of microcephaly were obtained from a Bayesian model which was conditional autoregressive (CAR) in space, and first order autoregressive in time. A total of 742 cases were identified. Additionally, high and early occurrences were identified in the Recife Metropolitan Region, on the coast, and in a north–south band about 300 km inland. Over a substantial part of the state, the overall prevalence, aggregating over the study period, was above 0.5%. The reasons for the high occurrence in the inland area remain unclear.
Spaces in Spatial Science and Urban Applications—State of the Art Review
Authors: Zlatanova Sisi , Yan Jinjin , Wang Yijing , Diakité Abdoulaye , Isikdag Umit , Sithole George , Barton Jack
Published: January 2020

In spatial science and urban applications, “space" is presented by multiple disciplines as a notion referencing our living environment. Space is used as a general term to help understand particular characteristics of the environment. However, the definition and perception of space varies and these variations have to be harmonised. For example, space may have diverse definitions and classification, the same environment may be abstracted/modelled by contradicting notions of space, which can lead to inconsistencies and misunderstandings. In this paper, we seek to investigate and document the state-of-the-art in the research of “space” regarding its definition, classification, modelling and utilization (2D/3D) in spatial sciences and urban applications. We focus on positioning, navigation, building micro-climate and thermal comfort, landscape, urban planning and design, urban heat island, interior design and planning, transportation and intelligent space. We review 147 research papers, technical reports and on-line resources. We compare the presented space concepts with respect to five criteria—classification, boundary, modelling components, use of standards and granularity. The review inventory is intended for both scientists and professionals in the spatial industry, such as companies, national mapping agencies and governments, and aim to provide a reference to better understand and employ the “space” while working across disciplines.
Acknowledgement to Reviewers of IJGI in 2019
Authors:   IJGI Editorial Office
Published: January 2020

The editorial team greatly appreciates the reviewers who have dedicated their considerable time and expertise to the journal’s rigorous editorial process over the past 12 months, regardless of whether the papers are finally published or not [...]
Integrative Analysis of Spatial Heterogeneity and Overdispersion of Crime with a Geographically Weighted Negative Binomial Model
Authors: Chen Jianguo , Liu Lin , Xiao Luzi , Xu Chong , Long Dongping
Published: January 2020

Negative binomial (NB) regression model has been used to analyze crime in previous studies. The disadvantage of the NB model is that it cannot deal with spatial effects. Therefore, spatial regression models, such as the geographically weighted Poisson regression (GWPR) model, were introduced to address spatial heterogeneity in crime analysis. However, GWPR could not account for overdispersion, which is commonly observed in crime data. The geographically weighted negative binomial model (GWNBR) was adopted to address spatial heterogeneity and overdispersion simultaneously in crime analysis, based on a 3-year data set collected from ZG city, China, in this study. The count of residential burglaries was used as the dependent variable to calibrate the above models, and the results revealed that the GWPR and GWNBR models performed better than NB for reducing spatial dependency in the model residuals. GWNBR outperformed GWPR for incorporating overdispersion. Therefore, GWNBR was proven to be a promising tool for crime modeling.
Towards Integrating Heterogeneous Data: A Spatial DBMS Solution from a CRC-LCL Project in Australia
Authors: Li Wei , Zlatanova Sisi , Diakite Abdoulaye A. , Aleksandrov Mitko , Yan Jinjin
Published: January 2020

Over recent decades, more and more cities worldwide have created semantic 3D city models of their built environments based on standards across multiple domains. 3D city models, which are often employed for a large range of tasks, go far beyond pure visualization. Due to different spatial scale requirements for planning and managing various built environments, integration of Geographic Information Systems (GIS) and Building Information Modeling (BIM) has emerged in recent years. Focus is now shifting to Precinct Information Modeling (PIM) which is in a more general sense to built-environment modeling. As scales change so do options to perform information modeling for different applications. How to implement data interoperability across these digital representations, therefore, becomes an emerging challenge. Moreover, with the growth of multi-source heterogeneous data consisting of semantic and varying 2D/3D spatial representations, data management becomes feasible for facilitating the development and deployment of PIM applications. How to use heterogeneous data in an integrating manner to further express PIM is an open and comprehensive topic. In this paper, we develop a semantic PIM based on multi-source heterogeneous data. Then, we tackle spatial data management problems in a Spatial Database Management System (SDBMS) solution for our defined unified model. Case studies on the University of New South Wales (UNSW) campus demonstrate the efficiency of our solution.
SLiX: A GIS Toolbox to Support Along-Stream Knickzones Detection through the Computation and Mapping of the Stream Length-Gradient (SL) Index
Authors: Piacentini Daniela , Troiani Francesco , Servizi Tommaso , Nesci Olivia , Veneri Francesco
Published: January 2020

The stream length-gradient (SL) index is widely used in geomorphological studies aimed at detecting knickzones, which are extensive along-stream deviations from the typical concave-up shape assumed for stream longitudinal profiles at steady-state conditions. In particular, SL was practical for identifying anomalous gradients along bedrock stream channels in mountainous catchments. This work presents the GIS toolbox SLiX designed to extract values of the SL index, starting from Digital Elevation Models (DEMs). SLiX is also suitable for the spatial analysis of the SL values, allowing for the identification of landscape portions where anomalous high values of SL occur and, consequently, those catchment sectors where stream channels show peaks in the erosional dynamic. The SLiX main outputs are (i) point shapefiles containing, among stream channels attributes, the extracted values of SL along the stream network analyzed, and (ii) SL anomaly maps in GeoTIFF format, computed through the Hotspot and Cluster Analysis (HCA), that permit the detection of the catchment sectors where the major SL anomalies occur and consequently the principal knickzones. The application of the proposed tool within an experimental catchment located in the Northern Apennines of Italy demonstrated the proper functionality and the potential of its use for different geomorphological and environmental studies. The accurate and cost-effective detection of anomalous changes in stream gradient ensured by SLiX is of great interest and can be useful for studies aimed at unravelling the Earth processes responsible of their formation (e.g., active hillslope processes, such as landslides directly interacting with the streambed, presence of geological structures, and meander cut-off). The applications of SLiX have clear implications for preliminary analyses, at a regional scale in different morpho-climatic contexts, for the hydrological management of river basins and/or to prevent geological hazards related to the fluvial erosional dynamics.
RETRACTED: Spatiotemporal Analysis of Tourists and Residents in Shanghai Based on Location-Based Social Network’s Data from Weibo
Authors: Khan Naimat , Wan Wanggen , Yu Shui
Published: January 2020

The aim of this study is to analyze and compare the patterns of behavior of tourists and residents from Location-Based Social Network (LBSN) data in Shanghai, China using various spatiotemporal analysis techniques at different venue categories. The paper presents the applications of location-based social network’s data by exploring the patterns in check-ins over a period of six months. We acquired the geo-location information from one of the most famous Chinese microblogs called Sina-Weibo (Weibo). The extracted data is translated into the Geographical Information Systems (GIS) format, and compared with the help of temporal statistical analysis and kernel density estimation. The venue classification is done by using information regarding the nature of physical locations. The findings reveal that the spatial activities of tourists are more concentrated as compared to those of residents, particularly in downtown, while the residents also visited suburban areas and the temporal activities of tourists varied significantly while the residents’ activities showed relatively stable behavior. These results can be applied in destination management, urban planning, and smart city development.
Determination of a Hazard Compensations Based on Land Administration Data
Authors: Vučić Nikola , Mađer Mario , Pivac Doris , Roić Miodrag , Križanović Josip
Published: January 2020

During the summer period, fire hazards represent an especially real threat not only for vegetation and animals, but also for material resources and human lives. In the Republic of Croatia, the most affected areas are those in the coastal region, which are characterised by a very dry environment and strong winds that are conducive to the rapid spread of wildfire. Although the prevention of wildfire is an utmost imperative, many countries are still missing a good and reliable fire management system for both fire monitoring and prevention and post-disaster management. The aim of this paper is to show the utilisation of open source GIS, web-based technologies, and open data in the case of natural disasters, and to define the concept of advanced national system of compensation determination. Such a system could be utilised to identify land parcels for which compensations for damage and losses after fire devastation have to be determined and could be used by compensation agencies, insurance companies, or state administration. The island of Hvar, which has suffered from fire hazards, was used as a case study for a quick and relevant calculation of the compensation based on land administration data. Sentinel satellite images and official digital orthophoto maps (DOMs) of the affected area before and after the fire devastation were obtained and analysed together with the data on cadastral parcels and land owners. Research was conducted by using both sets of imagery data, showing that Sentinel satellite images are more than adequate for fulfilling the task of the determination of hazard compensation.
Genome and Karyotype Reorganization after Whole Genome Duplication in Free-Living Flatworms of the Genus Macrostomum
Authors: Zadesenets Kira S. , Jetybayev Ilyas Y. , Schärer Lukas , Rubtsov Nikolay B.
Published: January 2020

The genus Macrostomum represents a diverse group of rhabditophoran flatworms with >200 species occurring around the world. Earlier we uncovered karyotype instability linked to hidden polyploidy in both M. lignano (2n = 8) and its sibling species M. janickei (2n = 10), prompting interest in the karyotype organization of close relatives. In this study, we investigated chromosome organization in two recently described and closely related Macrostomum species, M. mirumnovem and M. cliftonensis, and explored karyotype instability in laboratory lines and cultures of M. lignano (DV1/10, 2n = 10) and M. janickei in more detail. We revealed that three of the four studied species are characterized by karyotype instability, while M. cliftonensis showed a stable 2n = 6 karyotype. Next, we performed comparative cytogenetics of these species using fluorescent in situ hybridization (FISH) with a set of DNA probes (including microdissected DNA probes generated from M. lignano chromosomes, rDNA, and telomeric DNA). To explore the chromosome organization of the unusual 2n = 9 karyotype discovered in M. mirumnovem, we then generated chromosome-specific DNA probes for all chromosomes of this species. Similar to M. lignano and M. janickei, our findings suggest that M. mirumnovem arose via whole genome duplication (WGD) followed by considerable chromosome reshuffling. We discuss possible evolutionary scenarios for the emergence and reorganization of the karyotypes of these Macrostomum species and consider their suitability as promising animal models for studying the mechanisms and regularities of karyotype and genome evolution after a recent WGD.
Topical Therapy with Antisense Tumor Necrosis Factor Alpha Using Novel β-Glucan-Based Drug Delivery System Ameliorates Intestinal Inflammation
Authors: Sakisaka Hideto , Takedatsu Hidetoshi , Mitsuyama Keiichi , Mochizuki Shinichi , Sakurai Kazuo , Sakisaka Shotaro , Hirai Fumihito
Published: January 2020

Anti-tumor necrosis factor alpha (TNF-α) antibodies are effective in patients with inflammatory bowel disease (IBD). However, the effect is not optimal because a sufficient concentration of antibodies cannot be maintained at the site of inflammation. Thus, a macromolecular complex was developed with schizophyllan (SPG) and antisense oligonucleotides. In the present study, an SPG-antisense TNF-α complex was prepared, and its therapeutic efficacy was examined using a dextran sodium sulfate (DSS)-induced colitis model. The TNF-α production in CD11b+ macrophages significantly increased in the colon of DSS-treated mice. Dectin-1, a receptor of SPG, binds with SPG and is subsequently taken into the cells via phagocytosis. The expression of dectin-1 by CD11b+ macrophages significantly increased in DSS-treated mice. Flow cytometry revealed that the uptake of SPG-antisense TNF-α in the macrophages was efficient. TNF-α production was suppressed significantly by SPG-antisense TNF-α in vitro, which was administered via enema to evaluate its efficacy. The intrarectal administration of SPG-antisense TNF-α ameliorated the intestinal inflammation. In this study, we showed that the delivery system that conjugates SPG and antisense can have higher therapeutic efficacy. Thus, the new therapeutic approach presented in this study may be used in the management of IBD.
Computer-Aided Drug Design of β-Secretase, γ-Secretase and Anti-Tau Inhibitors for the Discovery of Novel Alzheimer’s Therapeutics
Authors: Mouchlis Varnavas D. , Melagraki Georgia , Zacharia Lefteris C. , Afantitis Antreas
Published: January 2020

Aging-associated neurodegenerative diseases, which are characterized by progressive neuronal death and synapses loss in human brain, are rapidly growing affecting millions of people globally. Alzheimer’s is the most common neurodegenerative disease and it can be caused by genetic and environmental risk factors. This review describes the amyloid-β and Tau hypotheses leading to amyloid plaques and neurofibrillary tangles, respectively which are the predominant pathways for the development of anti-Alzheimer’s small molecule inhibitors. The function and structure of the druggable targets of these two pathways including β-secretase, γ-secretase, and Tau are discussed in this review article. Computer-Aided Drug Design including computational structure-based design and ligand-based design have been employed successfully to develop inhibitors for biomolecular targets involved in Alzheimer’s. The application of computational molecular modeling for the discovery of small molecule inhibitors and modulators for β-secretase and γ-secretase is summarized. Examples of computational approaches employed for the development of anti-amyloid aggregation and anti-Tau phosphorylation, proteolysis and aggregation inhibitors are also reported.
Mechanisms and Treatment of Light-Induced Retinal Degeneration-Associated Inflammation: Insights from Biochemical Profiling of the Aqueous Humor
Authors: Chistyakov Dmitry V. , Baksheeva Viktoriia E. , Tiulina Veronika V. , Goriainov Sergei V. , Azbukina Nadezhda V. , Gancharova Olga S. , Arifulin Eugene A. , Komarov Sergey V. , Chistyakov Viktor V. , Tikhomirova Natalia K. , Zamyatnin Andrey A. , Philippov Pavel P. , Senin Ivan I. , Sergeeva Marina G. , Zernii Evgeni Yu.
Published: January 2020

Ocular inflammation contributes to the pathogenesis of blind-causing retinal degenerative diseases, such as age-related macular degeneration (AMD) or photic maculopathy. Here, we report on inflammatory mechanisms that are associated with retinal degeneration induced by bright visible light, which were revealed while using a rabbit model. Histologically and electrophysiologically noticeable degeneration of the retina is preceded and accompanied by oxidative stress and inflammation, as evidenced by granulocyte infiltration and edema in this tissue, as well as the upregulation of total protein, pro-inflammatory cytokines, and oxidative stress markers in aqueous humor (AH). Consistently, quantitative lipidomic studies of AH elucidated increase in the concentration of arachidonic (AA) and docosahexaenoic (DHA) acids and lyso-platelet activating factor (lyso-PAF), together with pronounced oxidative and inflammatory alterations in content of lipid mediators oxylipins. These alterations include long-term elevation of prostaglandins, which are synthesized from AA via cyclooxygenase-dependent pathways, as well as a short burst of linoleic acid derivatives that can be produced by both enzymatic and non-enzymatic free radical-dependent mechanisms. The upregulation of all oxylipins is inhibited by the premedication of the eyes while using mitochondria-targeted antioxidant SkQ1, whereas the accumulation of prostaglandins and lyso-PAF can be specifically suppressed by topical treatment with cyclooxygenase inhibitor Nepafenac. Interestingly, the most prominent antioxidant and anti-inflammatory benefits and overall retinal protective effects are achieved by simultaneous administrating of both drugs indicating their synergistic action. Taken together, these findings provide a rationale for using a combination of mitochondria-targeted antioxidant and cyclooxygenase inhibitor for the treatment of inflammatory components of retinal degenerative diseases.
Extremely Low Leakage Expression Systems Using Dual Transcriptional-Translational Control for Toxic Protein Production
Authors: Kato Yusuke
Published: January 2020

Expression systems for highly toxic protein genes must be conditional and suppress leakage expression to almost zero because even faint leakage expression may kill host cells, inhibit host growth, and cause loss of plasmids containing the toxic protein genes. The most widely used conditional expression systems are controlled only at the transcriptional level, and complete suppression of leakage expression is challenging. Recent progress on translational control has enabled construction of dual transcriptional-translational control systems in which leakage expression is strongly suppressed. This review summarizes the principles, features, and practical examples of dual transcriptional-translational control systems in bacteria, and provides future perspectives on these systems.
Nuclear Integrants of Organellar DNA Contribute to Genome Structure and Evolution in Plants
Authors: Zhang Guo-Jun , Dong Ran , Lan Li-Na , Li Shu-Fen , Gao Wu-Jun , Niu Hong-Xing
Published: January 2020

The transfer of genetic material from the mitochondria and plastid to the nucleus gives rise to nuclear integrants of mitochondrial DNA (NUMTs) and nuclear integrants of plastid DNA (NUPTs). This frequently occurring DNA transfer is ongoing and has important evolutionary implications. In this review, based on previous studies and the analysis of NUMT/NUPT insertions of more than 200 sequenced plant genomes, we analyzed and summarized the general features of NUMTs/NUPTs and highlighted the genetic consequence of organellar DNA insertions. The statistics of organellar DNA integrants among various plant genomes revealed that organellar DNA-derived sequence content is positively correlated with the nuclear genome size. After integration, the nuclear organellar DNA could undergo different fates, including elimination, mutation, rearrangement, fragmentation, and proliferation. The integrated organellar DNAs play important roles in increasing genetic diversity, promoting gene and genome evolution, and are involved in sex chromosome evolution in dioecious plants. The integrating mechanisms, involving non-homologous end joining at double-strand breaks were also discussed.
Nest Modification Protects Immature Stages of the Japanese Orchard Bee (Osmia cornifrons) from Invasion of a Cleptoparasitic Mite Pest
Authors: Joshi Neelendra K. , Naithani Kusum , Biddinger David J.
Published: January 2020

Osmia cornifrons (Radoszkowski) (Hymenoptera: Megachilidae) is an effective orchard pollinator. Considering the honey bee population decline in recent years, the conservation and propagation of O. cornifrons as an alternative managed pollinator is important in ensuring adequate pollination of tree fruit crops in the eastern United States. A field study was conducted to determine if nest modifications could reduce mite parasites and parasitoid natural enemies that attack managed O. cornifrons. Paraffin-coated paper liners (straws) were added to create modified nests, and were compared with the unmodified nests (i.e., nests without paper liners). In each nest, we recorded the number of nest cells with cleptoparasitic mites Chaetodactylus krombeini (Baker), and the presence of a parasitoid wasp Monodontomerus obscurus (Westwood). We also recorded the number of cocoons, male pupae, female pupae, and unconsumed pollen-nectar provision masses in these nests. Results showed that using paper liners in nest-tunnels greatly reduced the invasion of natural enemies of managed populations of O. cornifrons. Most notably, the addition of paper liners provided protection from invasion by C. krombeini mites, as the mean number of nest cells with mites were significantly lower in these lined nests compared to the nests without paper liners. A significantly higher number of male and female cocoons of O. cornifrons were recorded in the nests with paper liners. The population of M. obscurus was almost negligible in this field study. These results suggest that using nests with paper liners may accelerate the O. cornifrons population establishment and propagation in commercial orchards of rosaceous fruit crops, and possibly in other crops.
Survival and Recovery of the Pine-Tree Lappet Dendrolimus pini When Subjected to Simulated Starvation
Authors: Łukowski Adrian , Adamczyk Dawid , Karolewski Piotr
Published: January 2020

There are many reasons to study the survival and recovery of animals after starvation in simulated transport conditions or other passive dispersal methods. To do so, we chose Dendrolimus pini, an economically important pest of Scots pine with great potential in terms of passive dispersal outside its territory. In this work, we sought to answer the following questions: What is the maximum survival of different instar larvae after total starvation? Does access to dry tissues of the preferred host plant extend the lifespan of the larvae? Does the possibility of larvae recovery exist after starvation for various periods? We found that older larvae survived longer without food than younger larvae. Moreover, dry food did not extend the lifespan of the larvae. Our observations showed that insects were interested in food and tasted it at the beginning, but they did not feed on it for long. Furthermore, larvae recovery was indeed possible, and the time of starvation did not significantly affect this. We generally concluded that the D. pini larvae were characterized by the ability to survive without food for up to one month, which confirms that this species is able to survive long durations of transport to almost anywhere in the world.
Parasitoid Complex of Fall Armyworm, Spodoptera frugiperda, in Ghana and Benin
Authors: Agboyi Lakpo Koku , Goergen Georg , Beseh Patrick , Mensah Samuel Adjei , Clottey Victor Attuquaye , Glikpo Raymond , Buddie Alan , Cafà Giovanni , Offord Lisa , Day Roger , Rwomushana Ivan , Kenis Marc
Published: January 2020

The fall armyworm, Spodoptera frugiperda, a moth originating from the American continent, has recently invaded most African countries, where it is seriously threatening food security as a pest of cereals. The current management methods rely heavily on the use of synthetic insecticides but there is a need for more sustainable control methods, including biological control. Surveys were conducted in two West African countries, Ghana and Benin, to determine the native parasitoid complex and assess parasitism rates of S. frugiperda. Samples of S. frugiperda eggs and larvae were collected in maize fields located in 56 and 90 localities of Ghana and Benin, respectively, from July 2018 to July 2019. Ten species were found parasitizing the pest, including two egg parasitoids, one egg–larval, five larval and two larval–pupal parasitoids. The two most abundant parasitoids in both countries were two Braconidae: the egg-larval parasitoid Chelonus bifoveolatus and the larval parasitoid Coccygidum luteum. Parasitism rates were determined in three Ghanaian regions and averages varied from 0% to 75% between sites and from 5% to 38% between regions. These data provide an important baseline for the development of various biological control options. The two egg parasitoids, Telenomus remus and Trichogramma sp. can be used in augmentative biological control and investigations should be conducted to assess how cultural practices can enhance the action of the main parasitoids, C. luteum and Ch. bifoveolatus, in the field. Understanding the parasitoid complex of S. frugiperda in Africa is also necessary before any development of classical biological controls involving the introduction of parasitoids from the Americas.
Photosynthesis Inhibiting Effects of Pesticides on Sweet Pepper Leaves
Authors: Giménez–Moolhuyzen Miguel , van der Blom Jan , Lorenzo–Mínguez Pilar , Cabello Tomás , Crisol–Martínez Eduardo
Published: January 2020

Although a large number of pesticides of different compositions are regularly used in agriculture, the impact of pesticides on the physiology of field crops is not well understood. Pesticides can produce negative effects on crop physiology―especially on photosynthesis―leading to a potential decrease in both the growth and the yield of crops. To investigate these potential effects in greenhouse sweet peppers, the effect of 20 insecticides and 2 fungicides (each sprayed with a wetting agent) on the photosynthesis of sweet pepper leaves was analyzed. Among these pesticides, nine caused significant reductions in photosynthetic activity. The effects were observed in distinctive ways—either as a transitory drop of the photosynthetic-rate values, which was observed at two hours after the treatment and was found to have recovered after 24 h, or as a sustained reduction of these values, which remained substantial over a number of days. The results of this study suggest that the production of a crop may substantially benefit when the frequent use of pesticides can be substituted with alternative pest control methods (e.g., biological control). Our results advocate further investigation of the potential impact of pesticides, either alone or in combination, on the photosynthesis of crop plants.
Expression Dynamics of Core RNAi Machinery Genes in Pea Aphids Upon Exposure to Artificially Synthesized dsRNA and miRNAs
Authors: Yang Li , Tian Yuan , Peng Yuan-Yuan , Niu Jinzhi , Wang Jin-Jun
Published: January 2020

The pea aphid is an important pest of vegetables and causes serious losses worldwide. RNA interference (RNAi) is an effective pest control tool, and three sub-pathways have been described: The miRNA pathway, siRNA pathway, and piRNA pathway. A large number of genes in miRNA pathway and piRNA pathway are found to be expanded. To study the roles of these genes, the expression of 25 core RNAi genes was screened in spatiotemporal samples, artificially synthesized dsRNA and miRNA treated samples. The 25 genes were all expressed during different development stages and in different tissues. In dsRNA-treated samples and miRNA-treated samples, the expressions of genes in these three pathways were induced, especially the expanded genes. This suggests a complex network of RNAi core genes in the three sub-pathways. Treatment of miRNA seems to induce gene expression in a dosage-dependent manner. These results increase our knowledge of the siRNA pathway and related factors from RNAi pathway in aphids and promote the use of RNAi for the control of aphid pests.
Study of Two-Phase Newtonian Nanofluid Flow Hybrid with Hafnium Particles under the Effects of Slip
Authors: Ellahi Rahmat , Hussain Farooq , Asad Abbas Syed , Sarafraz Mohammad Mohsen , Goodarzi Marjan , Shadloo Mostafa Safdari
Published: January 2020

This paper investigates the role of slip in a two-phase flow of Newtonian fluid. The nano-size Hafnium particles are used in the base fluid. The fluid under consideration is studied for two cases namely (i) fluid phase (ii) phase of particles. Both cases are examined for three types of geometries. The governing equations are simplified in nondimensional form for each phase along with boundary conditions. The resulting equations have been analytically solved to get exact solutions for both fluid and particle phases. Different features of significant physical factors are discussed graphically. The flow patterns have been examined through streamlines.
Designing of Cost-Effective and Low-Carbon Multi-Energy Nanogrids for Residential Applications
Authors: Di Somma Marialaura , Caliano Martina , Graditi Giorgio , Pinnarelli Anna , Menniti Daniele , Sorrentino Nicola , Barone Giuseppe
Published: January 2020

Recently, hybrid multi-energy systems consisting of multiple generation, conversion, and storage technologies have been receiving great attention as a promising option to meet the multi-energy demands of residential end-users, by transforming them from passive consumers to active prosumers, who both produce and consume energy. The design problem of such systems is challenging due to the large number of degrees of freedom in the design and operation phases, so the system as a whole must be optimized. Moreover, both economic and low-carbon priorities should be considered in the design problem to foster an effective implementation and deployment. The aim of this paper was to present a methodology for the optimal design of multi-energy nanogrids (MENs) operating in grid-connected and islanded modes. Based on a pre-defined MEN superstructure, a multi-objective linear problem was established to find the types and sizes of the technologies in the MEN, with the aim to reduce the total annual cost and the fossil primary energy input, while satisfying the assigned time-varying user multi-energy demand. With reference to the latter, the thermal behavior of the building was simulated by using the dynamic simulation software TRNSYS. The Pareto frontier was found by minimizing a weighted sum of the total annual cost and fossil primary energy input, and the problem was solved by using branch-and-cut. In the numerical testing, a single-family house of 200 m2 located in Italy was considered as the residential end-user. Results show the effectiveness of the model for providing good balancing solutions for end-users based on economic and energetic priorities. Moreover, it was found that the MEN operating in grid-connected mode showed economic and environmental performances much better than those found for the configuration operating in islanded mode.