bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis and Evaluation of COVID-19 Web Applications for Health Professionals: Challenges and Opportunities
Authors: Mukhtar Hamid , Ahmad Hafiz , Khan Muhammad , Ullah Nasim
Published: November 2020

The multidisciplinary nature of the work required for research in the COVID-19 pandemic has created new challenges for health professionals in the battle against the virus. They need to be equipped with novel tools, applications, and resources—that have emerged during the pandemic—to gain access to breakthrough findings; know the latest developments; and to address their specific needs for rapid data acquisition, analysis, evaluation, and reporting. Because of the complex nature of the virus, healthcare systems worldwide are severely impacted as the treatment and the vaccine for COVID-19 disease are not yet discovered. This leads to frequent changes in regulations and policies by governments and international organizations. Our analysis suggests that given the abundance of information sources, finding the most suitable application for analysis, evaluation, or reporting, is one of such challenges. However, health professionals and policy-makers need access to the most relevant, reliable, trusted, and latest information and applications that can be used in their day-to-day tasks of COVID-19 research and analysis. In this article, we present our analysis of various novel and important web-based applications that have been specifically developed during the COVID-19 pandemic and that can be used by the health professionals community to help in advancing their analysis and research. These applications comprise search portals and their associated information repositories for literature and clinical trials, data sources, tracking dashboards, and forecasting models. We present a list of the minimally essential online, web-based applications to serve a multitude of purposes, from hundreds of those developed since the beginning of the pandemic. A critical analysis is provided for the selected applications based on 17 features that can be useful for researchers and analysts for their evaluations. These features make up our evaluation framework and have not been used previously for analysis and evaluation. Therefore, knowledge of these applications will not only increase productivity but will also allow us to explore new dimensions for using existing applications with more control, better management, and greater outcome of their research. In addition, the features used in our framework can be applied for future evaluations of similar applications and health professionals can adapt them for evaluation of other applications not covered in this analysis.
A Review of the Occurrence of Alpha-Emitting Radionuclides in Wild Mushrooms
Authors: Strumińska-Parulska Dagmara , Falandysz Jerzy
Published: November 2020

Alpha-emitting radioisotopes are the most toxic among all radionuclides. In particular, medium to long-lived isotopes of the heavier metals are of the greatest concern to human health and radiological safety. This review focuses on the most common alpha-emitting radionuclides of natural and anthropogenic origin in wild mushrooms from around the world. Mushrooms bio-accumulate a range of mineral ionic constituents and radioactive elements to different extents, and are therefore considered as suitable bio-indicators of environmental pollution. The available literature indicates that the natural radionuclide 210Po is accumulated at the highest levels (up to 22 kBq/kg dry weight (dw) in wild mushrooms from Finland), while among synthetic nuclides, the highest levels of up to 53.8 Bq/kg dw of 239+240Pu were reported in Ukrainian mushrooms. The capacity to retain the activity of individual nuclides varies between mushrooms, which is of particular interest for edible species that are consumed either locally or, in some cases, also traded on an international scale. The effective radiation dose from the ingestion of this food can reportedly range from 0.033 µSv/kg dw to 26.8 mSv/kg and varies depending on the country. Following pollution events, such consumption may expose consumers to highly radiotoxic decay particles produced by alpha emitters.
Comparative Analysis of PM2.5-Bound Polycyclic Aromatic Hydrocarbons (PAHs), Nitro-PAHs (NPAHs), and Water-Soluble Inorganic Ions (WSIIs) at Two Background Sites in Japan
Authors: Yang Lu , Zhang Lulu , Zhang Hao , Zhou Quanyu , Zhang Xuan , Xing Wanli , Takami Akinori , Sato Kei , Shimizu Atsushi , Yoshino Ayako , Kaneyasu Naoki , Matsuki Atsushi , Hayakawa Kazuichi , Toriba Akira , Tang Ning
Published: November 2020

Daily PM2.5 (particulate matter with aerodynamic diameter ≤2.5 μm) samples were simultaneously collected at two background sites (Wajima Air Monitoring Station (WAMS) and Fukue-Jima Atmosphere and Aerosol Monitoring Station (FAMS)) in Japan in the East Asian winter and summer monsoon periods of 2017 and 2019, to compare the characteristics of air pollutants among different regions and to determine the possible variation during the long-range transport process. Polycyclic aromatic hydrocarbons (PAHs), nitro-PAHs (NPAHs), and water-soluble inorganic ions (WSIIs) were analyzed. Despite the PM2.5 concentrations at FAMS (8.90–78.5 µg/m3) being higher than those at WAMS (2.33–21.2 µg/m3) in the winter monsoon period, the average concentrations of ∑PAHs, ∑NPAHs, and ∑WSIIs were similar between the two sites. Diagnostic ratios indicated PAHs mainly originated from traffic emissions and mostly aged, whereas NPAHs were mostly secondarily formed during long-range transport. WSIIs at WAMS were mainly formed via the combustion process and secondary reactions, whereas those at FAMS mainly originated from sea salt and dust. Backward trajectories revealed the air masses could not only come from Asian continental coastal regions but also distant landlocked areas in the winter monsoon period, whereas most came from the ocean in the summer monsoon period. These findings can provide basic data for the establishment of prediction models of transboundary air pollutants in East Asia.
Determination of the Optimum Removal Efficiency of Fine Particulate Matter Using Activated Carbon Fiber (ACF)
Authors: Kim Min-Kyeong , Jang Yelim , Park Duckshin
Published: November 2020

In Korea, concentrations of particulate matter (PM10) are significantly higher in urban railway tunnels (178.1 μg/m3) than in metropolitan areas (49 μg/m3). In railway tunnels in Korea, it was maintained at 3–4 times higher concentration than general atmosphere and platform. Dust generated by trains is scattered at high speed in these tunnels, making filtration difficult; therefore, the development of filters that can be maintained in tunnels is required. In the present study, we examined PM adsorption in the laboratory scale using activated carbon fiber (ACF), which has high adsorption and capacity. The ACF depth, velocity of flow, and fine PM concentration in the tunnel were the experimental variables. We compared PM concentrations before and after the filter experiments, and calculated removal efficiency to determine the optimal conditions. Comprehensive examination of the experimental variables and differential pressure showed that the optimal conditions for an ACF specimen were a wind speed of 3.0 m/s and the ACF depth of 400 mm. The average removal efficiency of PM10 was 55.5%, and that of PM2.5 was 36.6%. The reproducibility tests showed that the ACF filter could be washed and reused and is suitable for various places because it is easily maintained.
Nutritional Composition of Gluten-Free Labelled Foods in the Slovenian Food Supply
Authors: Lavriša Živa , Hribar Maša , Kušar Anita , Žmitek Katja , Pravst Igor
Published: November 2020

The market of gluten-free (GF) foods has been expanding in recent years. GF foods are consumed not only by those with medical predispositions for avoiding gluten, but also by a specific segment of consumers, searching for “healthier” food choices. For these, such practices can present a serious limitation in the variability of food choices. Considering that GF foods are commonly perceived as healthier alternatives, there is a lack of knowledge on the nutritional profile and content of specific nutrients of GF-labelled foods compared to general food supply. A comparison of nutritional composition of GF/non-GF packed foods in the Slovenian food supply was conducted. The nutrient profiling scoring criterion (NPSC) and content of specific nutrients/energy was compared between GF-labelled and regular foods. The highest proportion of GF-labelled products were found in food categories, which typically do not contain gluten (Cheese imitates, Milk imitates, Yoghurt imitates, Canned fish and seafood and Processed meat). Significant differences in the nutrient profile between GF-labelled and regular products were found in Cakes, muffins and pastry, Crisps and snacks, Desserts and Milk imitates. GF-labelled foods often had lower protein and sugar content. Energy value was comparable in most categories and no significant differences in salt content were found, compared to non-GF products. In conclusion, GF-labelled foods will unlikely bring health benefits to those who are not medically required to follow GF diet. Public health initiatives should aim towards promotion of consuming non-processed foods and provision of reliable information about who is required to consume GF foods.
Role of Nuclear Claudin-4 in Renal Cell Carcinoma
Authors: Owari Takuya , Sasaki Takamitsu , Fujii Kiyomu , Fujiwara-Tani Rina , Kishi Shingo , Mori Shiori , Mori Takuya , Goto Kei , Kawahara Isao , Nakai Yasushi , Miyake Makito , Luo Yi , Tanaka Nobumichi , Kondoh Masuo , Fujimoto Kiyohide , Kuniyasu Hiroki
Published: November 2020

Claudin-4 (CLDN4) is a tight junction protein to maintain the cancer microenvironment. We recently reported the role of the CLDN4 not forming tight junction in the induction of epithelial-mesenchymal transition (EMT). Herein, we investigated the role of CLDN4 in renal cell carcinoma (RCC), focusing on CLDN4. CLDN4 expression in 202 RCCs was examined by immunostaining. CLDN4 phosphorylation and subcellular localization were examined using high metastatic human RCC SN12L1 and low metastatic SN12C cell lines. In 202 RCC cases, the CLDN4 expression decreased in the cell membrane and had no correlation with clinicopathological factors. However, CLDN4 was localized in the nucleus in 5 cases (2%), all of which were pT3. Contrastingly, only 6 of 198 nuclear CLDN4-negative cases were pT3. CLDN4 was found in the nuclear fraction of a highly metastatic human RCC cell line, SN12L1, but not in the low metastatic SN12C cells. In SN12L1 cells, phosphorylation of tyrosine and serine residues was observed in cytoplasmic CLDN4, but not in membranous CLDN4. In contrast, phosphorylation of serine residues was observed in nuclear CLDN4. In SN12L1 cells, CLDN4 tyrosine phosphorylation by EphA2/Ephrin A1 resulted in the release of CLDN4 from tight junction and cytoplasmic translocation. Furthermore, protein kinase C (PKC)-ε phosphorylated the CLDN4 serine residue, resulting in nuclear import. Contrarily, in SN12C cells that showed decreased expression of EphA2/Ephrin A1 and PKCε, the activation of EphA2/EphrinA1 and PKCε induced cytoplasmic and nuclear translocation of CLDN4, respectively. Furthermore, the nuclear translocation of CLDN4 promoted the nuclear translocation of Yes-associated protein (YAP) bound to CLDN4, which induced the EMT phenotype. These findings suggest that the release of CLDN4 by impaired tight junction might be a mechanism underlying the malignant properties of RCC. These findings suggest that the release of CLDN4 by impaired tight junction might be one of the mechanisms of malignant properties of RCC.
3D Spheroids Derived from Human Lipedema ASCs Demonstrated Similar Adipogenic Differentiation Potential and ECM Remodeling to Non-Lipedema ASCs In Vitro
Authors: Al-Ghadban Sara , Pursell India A. , Diaz Zaidmara T. , Herbst Karen L. , Bunnell Bruce A.
Published: November 2020

The growth and differentiation of adipose tissue-derived stem cells (ASCs) is stimulated and regulated by the adipose tissue (AT) microenvironment. In lipedema, both inflammation and hypoxia influence the expansion and differentiation of ASCs, resulting in hypertrophic adipocytes and deposition of collagen, a primary component of the extracellular matrix (ECM). The goal of this study was to characterize the adipogenic differentiation potential and assess the levels of expression of ECM-remodeling markers in 3D spheroids derived from ASCs isolated from both lipedema and healthy individuals. The data showed an increase in the expression of the adipogenic genes (ADIPOQ, LPL, PPAR-γ and Glut4), a decrease in matrix metalloproteinases (MMP2, 9 and 11), with no significant changes in the expression of ECM markers (collagen and fibronectin), or integrin A5 in 3D differentiated lipedema spheroids as compared to healthy spheroids. In addition, no statistically significant changes in the levels of expression of inflammatory genes were detected in any of the samples. However, immunofluorescence staining showed a decrease in fibronectin and increase in laminin and Collagen VI expression in the 3D differentiated spheroids in both groups. The use of 3D ASC spheroids provide a functional model to study the cellular and molecular characteristics of lipedema AT.
Fragmentation Study, Dual Anti-Bactericidal and Anti-Viral Effects and Molecular Docking of Cobalt(III) Complexes
Authors: Fernandes Laísa de P. , Silva Júlia M. B. , Martins Daniel O. S. , Santiago Mariana B. , Martins Carlos H. G. , Jardim Ana C. G. , Oliveira Guedmiller S. , Pivatto Marcos , Souza Rafael A. C. , Franca Eduardo de F. , Deflon Victor M. , Machado Antonio E. H. , Oliveira Carolina G.
Published: November 2020

Considering our previous findings on the remarkable activity exhibited by cobalt(III) with 2-acetylpyridine-N(4)-R-thiosemicarbazone (Hatc-R) compounds against Mycobacterium tuberculosis, the present study aimed to explored new structure features of the complexes of the type [Co(atc--R)2]Cl, where R = methyl (Me, 1) or phenyl (Ph, 2) (13C NMR, high-resolution mass spectrometry, LC–MS/MS, fragmentation study) together with its antibacterial and antiviral biological activities. The minimal inhibitory and minimal bactericidal concentrations (MIC and MBC) were determined, as well as the antiviral potential of the complexes on chikungunya virus (CHIKV) infection in vitro and cell viability. [Co(atc-Ph)2]Cl revealed promising MIC and MBC values which ranged from 0.39 to 0.78 µg/mL in two strains tested and presented high potential against CHIKV by reducing viral replication by up to 80%. The results showed that the biological activity is strongly influenced by the peripheral substituent groups at the N(4) position of the atc-R1− ligands. In addition, molecular docking analysis was performed. The relative binding energy of the docked compound with five bacteria strains was found in the range of −3.45 and −9.55 kcal/mol. Thus, this work highlights the good potential of cobalt(III) complexes and provide support for future studies on this molecule aiming at its antibacterial and antiviral therapeutic application.
Characterization of PC12 Cell Subclones with Different Sensitivities to Programmed Thermal Stimulation
Authors: Kudo Tada-aki , Tominami Kanako , Izumi Satoshi , Hayashi Yohei , Noguchi Takuya , Matsuzawa Atsushi , Hong Guang , Nakai Junichi
Published: November 2020

Neuritogenesis is the process underling nervous system regeneration; however, optimal extracellular signals that can promote neuronal regenerative activities require further investigation. Previously, we developed a novel method for inducing neuronal differentiation in rat PC12 cells using temperature-controlled repeated thermal stimulation (TRTS) with a heating plate. Based on neurogenic sensitivity to TRTS, PC12 cells were classified as either hyper- or hyposensitive. In this study, we aimed to investigate the mechanism of hyposensitivity by establishing two PC12-derived subclones according to TRTS sensitivity during differentiation: PC12-P1F1, a hypersensitive subclone, and PC12-P1D10, a hyposensitive subclone. To characterize these subclones, cell size and neuritogenesis were evaluated in subclones treated with nerve growth factor (NGF), bone morphogenetic protein (BMP), or various TRTS. No significant differences in cell size were observed among the parental cells and subclones. BMP4- or TRTS-induced neuritogenesis was increased in PC12-P1F1 cells compared to that in the parental cells, while no neuritogenesis was observed in PC12-P1D10 cells. In contrast, NGF-induced neuritogenesis was observed in all three cell lines. Furthermore, a BMP inhibitor, LDN-193189, considerably inhibited TRTS-induced neuritogenesis. These results suggest that the BMP pathway might be required for TRTS-induced neuritogenesis, demonstrating the useful aspects of these novel subclones for TRTS research.
Cardiovascular Active Peptides of Marine Origin with ACE Inhibitory Activities: Potential Role as Anti-Hypertensive Drugs and in Prevention of SARS-CoV-2 Infection
Authors: Festa Marco , Sansone Clementina , Brunet Christophe , Crocetta Fabio , Di Paola Luisa , Lombardo Michele , Bruno Antonino , Noonan Douglas M. , Albini Adriana
Published: November 2020

Growing interest in hypertension—one of the main factors characterizing the cardiometabolic syndrome (CMS)—and anti-hypertensive drugs raised from the emergence of a new coronavirus, SARS-CoV-2, responsible for the COVID19 pandemic. The virus SARS-CoV-2 employs the Angiotensin-converting enzyme 2 (ACE2), a component of the RAAS (Renin-Angiotensin-Aldosterone System) system, as a receptor for entry into the cells. Several classes of synthetic drugs are available for hypertension, rarely associated with severe or mild adverse effects. New natural compounds, such as peptides, might be useful to treat some hypertensive patients. The main feature of ACE inhibitory peptides is the location of the hydrophobic residue, usually Proline, at the C-terminus. Some already known bioactive peptides derived from marine resources have potential ACE inhibitory activity and can be considered therapeutic agents to treat hypertension. Peptides isolated from marine vertebrates, invertebrates, seaweeds, or sea microorganisms displayed important biological activities to treat hypertensive patients. Here, we reviewed the anti-hypertensive activities of bioactive molecules isolated/extracted from marine organisms and discussed the associated molecular mechanisms involved. We also examined ACE2 modulation in sight of SARS2-Cov infection prevention.
Adipokines, Myokines, and Cardiokines: The Role of Nutritional Interventions
Authors: Senesi Pamela , Luzi Livio , Terruzzi Ileana
Published: November 2020

It is now established that adipose tissue, skeletal muscle, and heart are endocrine organs and secrete in normal and in pathological conditions several molecules, called, respectively, adipokines, myokines, and cardiokines. These secretory proteins constitute a closed network that plays a crucial role in obesity and above all in cardiac diseases associated with obesity. In particular, the interaction between adipokines, myokines, and cardiokines is mainly involved in inflammatory and oxidative damage characterized obesity condition. Identifying new therapeutic agents or treatment having a positive action on the expression of these molecules could have a key positive effect on the management of obesity and its cardiac complications. Results from recent studies indicate that several nutritional interventions, including nutraceutical supplements, could represent new therapeutic agents on the adipo-myo-cardiokines network. This review focuses the biological action on the main adipokines, myokines and cardiokines involved in obesity and cardiovascular diseases and describe the principal nutraceutical approaches able to regulate leptin, adiponectin, apelin, irisin, natriuretic peptides, and follistatin-like 1 expression.
Comparative Transcriptome Analysis of Rutabaga (Brassica napus) Cultivars Indicates Activation of Salicylic Acid and Ethylene-Mediated Defenses in Response to Plasmodiophora brassicae
Authors: Zhou Qinqin , Galindo-González Leonardo , Manolii Victor , Hwang Sheau-Fang , Strelkov Stephen E.
Published: November 2020

Clubroot, caused by Plasmodiophora brassicae Woronin, is an important soilborne disease of Brassica napus L. and other crucifers. To improve understanding of the mechanisms of resistance and pathogenesis in the clubroot pathosystem, the rutabaga (B. napus subsp. rapifera Metzg) cultivars ‘Wilhelmsburger’ (resistant) and ‘Laurentian’ (susceptible) were inoculated with P. brassicae pathotype 3A and their transcriptomes were analyzed at 7, 14, and 21 days after inoculation (dai) by RNA sequencing (RNA-seq). Thousands of transcripts with significant changes in expression were identified in each host at each time-point in inoculated vs. non-inoculated plants. Molecular responses at 7 and 14 dai supported clear differences in the clubroot response mechanisms of the two genotypes. Both the resistant and the susceptible cultivars activated receptor-like protein (RLP) genes, resistance (R) genes, and genes involved in salicylic acid (SA) signaling as clubroot defense mechanisms. In addition, genes related to calcium signaling and genes encoding leucine-rich repeat (LRR) receptor kinases, the respiratory burst oxidase homolog (RBOH) protein, and transcription factors such as WRKYs, ethylene responsive factors, and basic leucine zippers (bZIPs), appeared to be upregulated in ‘Wilhelmsburger’ to restrict P. brassicae development. Some of these genes are essential components of molecular defenses, including ethylene (ET) signaling and the oxidative burst. Our study highlights the importance of activation of genes associated with SA- and ET-mediated responses in the resistant cultivar. A set of candidate genes showing contrasting patterns of expression between the resistant and susceptible cultivars was identified and includes potential targets for further study and validation through approaches such as gene editing.
The Role of Foot-Loading Factors and Their Associations with Ulcer Development and Ulcer Healing in People with Diabetes: A Systematic Review
Authors: Hulshof Chantal M. , van Netten Jaap J. , Pijnappels Mirjam , Bus Sicco A.
Published: November 2020

We aimed to comprehensively and systematically review studies associating key foot-loading factors (i.e., plantar pressure, weight-bearing activity, adherence or a combination thereof) with ulcer development and ulcer healing in people with diabetes. A systematic literature search was performed in PubMed and EMBASE. We included studies if barefoot or in-shoe plantar pressure, weight-bearing activity or footwear or device adherence was measured and associated with either ulcer development or ulcer healing in people with diabetes. Out of 1954 records, 36 studies were included and qualitatively analyzed. We found low to moderate quality evidence that lower barefoot plantar pressure and higher footwear and device adherence associate with lower risk of ulcer development and shorter healing times. For the other foot-loading factors, we found low quality evidence with limited or contradictory results. For combined measures of foot-loading factors, we found low quality evidence suggesting that lower cumulative plantar tissue stress is associated with lower risk of ulcer development and higher ulcer healing incidence. We conclude that evidence for barefoot plantar pressure and adherence in association with ulcer outcome is present, but is limited for the other foot-loading factors. More comprehensive investigation in particularly the combination of foot-loading factors may improve the evidence and targeting preventative treatment.
Fat Embolism Syndrome in Sickle Cell Disease
Authors: Tsitsikas Dimitris A. , Bristowe Jessica , Abukar Jibril
Published: November 2020

Fat embolism syndrome is a devastating complication of sickle cell disease resulting from extensive bone marrow necrosis and associated with high mortality rates, while survivors often suffer severe neurological sequelae. Despite that, the syndrome remains under-recognised and under-diagnosed. Paradoxically, it affects exclusively patients with mild forms of sickle cell disease, predominantly HbSC and HbSβ+. A significant number of cases occur in the context of human parvovirus B19 infection. We provide here a brief summary of the existing literature and describe our experience treating 8 patients in our institution. One patient had HbSS, 6 HbSC and 1 HbSβ+. All patients developed type I respiratory failure and neurological involvement either at presentation or within the first 72 h. The most striking laboratory abnormality was a 100-fold increase of the serum ferritin from baseline. Seven patients received emergency red cell exchange and 1 simple transfusion. Two patients (25%) died, 2 patients (25%) suffered severe neurological impairment and 1 (12%) mild neurological impairment on discharge, while 3 (38%) patients made a complete recovery. With long-term follow-up, 1 patient with severe neurological impairment and one patient with mild neurological impairment made dramatic improvements, making the long-term complete recovery or near complete recovery rate 63%. Immediate red cell exchange transfusion can be lifesaving and should be instituted as soon as the syndrome is suspected. However, as the outcomes remain unsatisfactory despite the increasing use of red cell exchange, we suggest additional therapeutic measures such as therapeutic plasma exchange and pre-emptive transfusion for high risk patients.
Immunofluorescence Evaluation of Myf5 and MyoD in Masseter Muscle of Unilateral Posterior Crossbite Patients
Authors: Vermiglio Giovanna , Centofanti Antonio , Ramieri Guglielmo , Tepedino Michele , Runci Anastasi Michele , Micali Antonio Girolamo , Arco Alba , Piancino Maria Grazia
Published: November 2020

A unilateral posterior crossbite is a malocclusion where the low activity of the affected masseter muscle is compensated by the contralateral muscle hypertrophy. It is still unknown if, in the same condition, myogenesis with new fibre formation takes place. Aim: the aim of the present study was to evaluate the expression of myogenesis markers, such as Myf5 and MyoD, in masseter muscles of unilateral posterior crossbite patients. Materials and methods: biopsies from fifteen surgical patients with unilateral posterior crossbites have been analysed by immunofluorescence reactions. The results show the expression of Myf5 and MyoD in the contralateral muscle but not in the ipsilateral one. Moreover, statistical analysis shows the higher number of satellite cells in the contralateral side if compared to the ipsilateral one. Conclusions: these results suggest that in contralateral muscle, hyperplastic events take place, as well as hypertrophy.
Assessment of Building Vulnerability to Tsunami Hazard in Kamari (Santorini Island, Greece)
Authors: Batzakis Dimitrios-Vasileios , Misthos Loukas-Moysis , Voulgaris Gerasimos , Tsanakas Konstantinos , Andreou Maria , Tsodoulos Ioannis , Karymbalis Efthimios
Published: November 2020

Santorini Island, located in the Southern Aegean Sea, is prone to tsunamis due to its proximity to the Hellenic subduction zone, which is one of the major tsunamigenic areas. Characteristic events, such as those of 365 A.D. and 1303 A.D. greatly affected the coasts of the Eastern Mediterranean Sea, causing significant loss of life and construction damage. Tsunami disaster risk is nowadays significantly higher due to the increased exposure of the buildings as a result of the economic and touristic growth of the Aegean Islands. This study focuses on the eastern coast of Santorini, since its morphology and human presence amplify the necessity to assess its building vulnerability. After conducting an exposure analysis at the settlements of the eastern coast, Kamari poses the highest physical, social and economic relative exposure to any potential natural hazard. The main objective of this research is to quantify the building stock’s vulnerability to tsunami hazard. For this purpose, a “worst-case run-up scenario” was developed. Considering the history of tsunamis in the Aegean Sea, an extreme sea-level rise after a 10 m a.s.l. tsunami run-up, caused by an earthquake with Mw~8.5, was assumed. The relative vulnerability of the buildings in Kamari was calculated via the application of the Papathoma Tsunami Vulnerability Assessment (PTVA-4) analytic model. The results indicate that 423 buildings are within the inundation zone, 58% of which are characterized as highly and very highly vulnerable to tsunamis, revealing the problematic characteristics of the building stock, offering important information to the decision-makers to mitigate a possible future tsunami impact.
Effect of UV and Visible Radiation on Optical Properties of Chromophoric Dissolved Organic Matter Released by Emiliania huxleyi
Authors: Retelletti Brogi Simona , Charrière Bruno , Gonnelli Margherita , Vaultier Frédéric , Sempéré Richard , Vestri Stefano , Santinelli Chiara
Published: November 2020

Photodegradation is a natural process that strongly affects the chromophoric fraction of dissolved organic matter (DOM), especially in surface water of the oceans. In the euphotic zone, the concentration and quality of DOM are mostly dependent on primary production by phytoplankton. The effect of photodegradation on algal DOM has not been investigated as much as on terrestrial DOM. In this study, we explored the effect of different spectral regions (i.e., full sun spectrum, visible light, 295–800 nm, 305–800 nm, and 320–800 nm) on algal exudates by Emiliania huxleyi, a ubiquitous coccolithophore. The optical properties (absorption and fluorescence) of algal DOM were investigated before and after irradiation with the different spectral regions. The absorption and fluorescence spectra were compared before and after irradiation. The results showed an increase in the effect of photobleaching with increasing irradiation energy for all of the absorbance indices. Similarly, the protein-like fluorescence decreased at increasing irradiation energy. The humic-like fluorescence, which was the most affected, did not show a linear trend between photobleaching and irradiation energy, which suggested that irradiation mainly determined a change in these molecules’ quantum yield.
From Ripples to Large-Scale Sand Transport: The Effects of Bedform-Related Roughness on Hydrodynamics and Sediment Transport Patterns in Delft3D
Authors: Brakenhoff Laura , Schrijvershof Reinier , van der Werf Jebbe , Grasmeijer Bart , Ruessink Gerben , van der Vegt Maarten
Published: November 2020

Bedform-related roughness affects both water movement and sediment transport, so it is important that it is represented correctly in numerical morphodynamic models. The main objective of the present study is to quantify for the first time the importance of ripple- and megaripple-related roughness for modelled hydrodynamics and sediment transport on the wave- and tide-dominated Ameland ebb-tidal delta in the north of the Netherlands. To do so, a sensitivity analysis was performed, in which several types of bedform-related roughness predictors were evaluated using a Delft3D model. Also, modelled ripple roughness was compared to data of ripple heights observed in a six-week field campaign on the Ameland ebb-tidal delta. The present study improves our understanding of how choices in model set-up influence model results. By comparing the results of the model scenarios, it was found that the ripple and megaripple-related roughness affect the depth-averaged current velocity, mainly over the shallow areas of the delta. The small-scale ripples are also important for the suspended load sediment transport, both indirectly through the affected flow and directly. While the current magnitude changes by 10–20% through changes in bedform roughness, the sediment transport magnitude changes by more than 100%.
Torsional and Transversal Stiffness of Orthotropic Sandwich Panels
Authors: Garbowski Tomasz , Gajewski Tomasz , Grabski Jakub Krzysztof
Published: November 2020

In the present work, an analytical equation describing the plate torsion test taking into account the transverse shear stiffness in sandwich plates is derived and numerically validated. Transverse shear becomes an important component if the analyzed plate or shell is thick with respect to the in-plane dimensions and/or its core has significantly lower stiffness than the outer faces. The popular example of such a sandwich plate is a corrugated cardboard, widely used in the packaging industry. The flat layers of a corrugated board are usually made of thicker (stronger) material than that used for the corrugated layer, the role of which is rather to keep the outer layers at a certain distance, to ensure high bending stiffness of the plate. However, the soft core of such a plate usually has a low transverse shear stiffness, which is often not considered in the plate analysis. Such simplification may lead to significant calculation errors. The paper presents the generalization of the Reissner’s analytical formula, which describes the torsional stiffness of the plate sample including two transverse shear stiffnesses. The paper also presents the implementation of the numerical model of the plate torsion test including the transverse shear stiffnesses. Both approaches are compared with each other on a wide range of material parameters and different aspect ratios of the specimen. It has been proved that both analytical and numerical formulations lead to an identical result. Finally, the performance of presented formulations is compared with other numerical models using commercial implementation of various Reissner–Mindlin shell elements and other analytical formulas from the literature. The comparison shows good agreement of presented theory and numerical implementation with other existing approaches.
Investigations of Liquid Steel Viscosity and Its Impact as the Initial Parameter on Modeling of the Steel Flow through the Tundish
Authors: Ślęzak Marta , Warzecha Marek
Published: November 2020

The paper presents research carried out to experimentally determine the dynamic viscosity of selected iron solutions. A high temperature rheometer with an air bearing was used for the tests, and ANSYS Fluent commercial software was used for numerical simulations. The experimental results obtained are, on average, lower by half than the values of the dynamic viscosity coefficient of liquid steel adopted during fluid flow modeling. Numerical simulations were carried out, taking into account the viscosity standard adopted for most numerical calculations and the average value of the obtained experimental dynamic viscosity of the analyzed iron solutions. Both qualitative and quantitative analysis showed differences in the flow structure of liquid steel in the tundish, in particular in the predicted values and the velocity profile distribution. However, these differences are not significant. In addition, the work analyzed two different rheological models—including one of our own—to describe the dynamic viscosity of liquid steel, so that in the future, the experimental stage could be replaced by calculating the value of the dynamic viscosity coefficient of liquid steel using one equation. The results obtained support the use of the author’s rheological model for the above; however, this model still needs to be refined and extended to a wide range of alloying elements, mainly the extension of the carbon range.
The Study of Structural, Impedance and Energy Storage Behavior of Plasticized PVA:MC Based Proton Conducting Polymer Blend Electrolytes
Authors: Aziz Shujahadeen B. , Brevik Iver , Brza M. A. , Asnawi A. S. F. M. , Dannoun Elham M. A. , Yusof Y. M. , Abdulwahid Rebar T. , Hamsan M. H. , M. Nofal Muaffaq , Kadir M. F. Z.
Published: November 2020

In this study, structural characterization, electrical properties and energy storage performance of plasticized polymer electrolytes based on polyvinyl alcohol/methylcellulose/ammonium thiocyanate (PVA/MC-NH4SCN) were carried out. An X-ray diffraction (XRD) study displayed that the plasticized electrolyte system with the uppermost value of direct current (DC) ionic conductivity was the most amorphous system. The electrolyte in the present work realized an ionic conductivity of 2.903 × 10−3 Scm−1 at room temperature. The main charge carrier in the electrolyte was found to be the ions with the ionic transference number (tion) of 0.912, compared to only 0.088 for the electronic transference number (telec). The electrochemical stability potential window of the electrolyte is 2.1 V. The specific capacitance was found to reduce from 102.88 F/g to 28.58 F/g as the scan rate increased in cyclic voltammetry (CV) analysis. The fabricated electrochemical double layer capacitor (EDLC) was stable up to 200 cycles with high efficiency. The specific capacitance obtained for the EDLC by using charge–discharge analysis was 132.7 F/g at the first cycle, which is slightly higher compared to the CV plot. The equivalent series resistance (ESR) increased from 58 to 171 Ω throughout the cycles, which indicates a good electrolyte/electrode contact. Ions in the electrolyte were considered to have almost the same amount of energy during the conduction process as the energy density is approximately at 14.0 Wh/kg throughout the 200 cycles. The power density is stabilized at the range of 1444.3 to 467.6 W/kg as the EDLC completed the cycles.
A Novel, Nontoxic and Scalable Process to Produce Lipidic Vehicles
Authors: Naziris Nikolaos , Pippa Natassa , Demetzos Costas
Published: November 2020

Lipidic vehicles are novel industrial products, utilized as components for pharmaceutical, cosmeceutical and nutraceutical formulations. The present study concerns a newly invented method to produce lipidic vehicles in the nanoscale that is simple, nontoxic, versatile, time-efficient, low-cost and easy to scale up. The process is a modification of the heating method (MHM) and comprises (i) providing a mixture of an amphiphilic lipid and a charged lipid and/or a fluidity regulator in a liquid medium composed of water and a liquid polyol, (ii) stirring and heating the mixture in two heating steps, wherein the temperature of the second step is higher than the temperature of the first step and (iii) allowing the mixture to cool down to room temperature. The process leads to the self-assembly of nanoparticles of small size and good homogeneity, compared with conventional approaches that require additional size reduction steps. In addition, the incorporation of bioactive molecules, such as drugs, inside the nanoparticles is possible, while lyophilization of the products provides long-term stability. Most importantly, the absence of toxic solvents and the simplicity guarantee the safety and scalability of the process, distinguishing it from most prior art processes to produce lipidic vehicles.
Inequalities in Triangular Norm-Based ∗-fuzzy ( L + ) p Spaces
Authors: Ghaffari Abbas , Saadati Reza , Mesiar Radko
Published: November 2020

In this article, we introduce the ∗-fuzzy (L+)p spaces for 1≤p<∞ on triangular norm-based ∗-fuzzy measure spaces and show that they are complete ∗-fuzzy normed space and investigate some properties in these space. Next, we prove Chebyshev’s inequality and Hölder’s inequality in ∗-fuzzy (L+)p spaces.
A Robust and Reversible Watermarking Algorithm for a Relational Database Based on Continuous Columns in Histogram
Authors: Li Yan , Wang Junwei , Jia Hongyong
Published: November 2020

Due to the discreteness of integer data, there are a large number of gaps and continuous columns in the histogram based on integer data. Aiming at the characteristics, this paper presents a robust and reversible watermarking algorithm for a relational database based on continuous columns in histogram. Firstly, it groups the database tuples according to the watermark length and the grouping key. Secondly, it calculates the prediction errors and uses the absolute values of the prediction errors to construct the histogram. Thirdly, it traverses the histogram to find all the continuous columns and in turn, computes the sum of the height of each continuous column and selects the group of continuous columns that has the largest sum as the positions to embed the watermarks. FCTD (Forest cover type data set) is utilized for experimental verification. A large amount of experimental data shows that the method is effective and robust. Not only does the data distortion caused by shifting histogram columns not exist, but the robustness of the watermark is also greatly improved.
Mitochondrial Targeting in an Anti-Austerity Approach Involving Bioactive Metabolites Isolated from the Marine-Derived Fungus Aspergillus sp.
Authors: Abdel-Naime Waleed A , Kimishima Atsushi , Setiawan Andi , Fahim John Refaat , Fouad Mostafa A. , Kamel Mohamed Salah , Arai Masayoshi
Published: November 2020

The tumor microenvironment is a nutrient-deficient region that alters the cancer cell phenotype to aggravate cancer pathology. The ability of cancer cells to tolerate nutrient starvation is referred to as austerity. Compounds that preferentially target cancer cells growing under nutrient-deficient conditions are being employed in anti-austerity approaches in anticancer drug discovery. Therefore, in this study, we investigated physcion (1) and 2-(2′,3-epoxy-1′,3′,5′-heptatrienyl)-6-hydroxy-5-(3-methyl-2-butenyl) benzaldehyde (2) obtained from a culture extract of the marine-derived fungus Aspergillus species (sp.), which were isolated from an unidentified marine sponge, as anti-austerity agents. The chemical structures of 1 and 2 were determined via spectroscopic analysis and comparison with authentic spectral data. Compounds 1 and 2 exhibited selective cytotoxicity against human pancreatic carcinoma PANC-1 cells cultured under glucose-deficient conditions, with IC50 values of 6.0 and 1.7 µM, respectively. Compound 2 showed higher selective growth-inhibitory activity (505-fold higher) under glucose-deficient conditions than under general culture conditions. Further analysis of the mechanism underlying the anti-austerity activity of compounds 1 and 2 against glucose-starved PANC-1 cells suggested that they inhibited the mitochondrial electron transport chain.
Microbial Species–Area Relationships in Antarctic Cryoconite Holes Depend on Productivity
Authors: Sommers Pacifica , Porazinska Dorota L. , Darcy John L. , Gendron Eli M. S. , Vimercati Lara , Solon Adam J. , Schmidt Steven K.
Published: November 2020

The island species–area relationship (ISAR) is a positive association between the number of species and the area of an isolated, island-like habitat. ISARs are ubiquitous across domains of life, yet the processes generating ISARs remain poorly understood, particularly for microbes. Larger and more productive islands are hypothesized to have more species because they support larger populations of each species and thus reduce the probability of stochastic extinctions in small population sizes. Here, we disentangled the effects of “island” size and productivity on the ISAR of Antarctic cryoconite holes. We compared the species richness of bacteria and microbial eukaryotes on two glaciers that differ in their productivity across varying hole sizes. We found that cryoconite holes on the more productive Canada Glacier gained more species with increasing hole area than holes on the less productive Taylor Glacier. Within each glacier, neither productivity nor community evenness explained additional variation in the ISAR. Our results are, therefore, consistent with productivity shaping microbial ISARs at broad scales. More comparisons of microbial ISARs across environments with limited confounding factors, such as cryoconite holes, and experimental manipulations within these systems will further contribute to our understanding of the processes shaping microbial biogeography.
Diversify and Conquer: The Vaccine Escapism of Plasmodium falciparum
Authors: Pance Alena
Published: November 2020

Over the last century, a great deal of effort and resources have been poured into the development of vaccines to protect against malaria, particularly targeting the most widely spread and deadly species of the human-infecting parasites: Plasmodium falciparum. Many of the known proteins the parasite uses to invade human cells have been tested as vaccine candidates. However, precisely because of the importance and immune visibility of these proteins, they tend to be very diverse, and in many cases redundant, which limits their efficacy in vaccine development. With the advent of genomics and constantly improving sequencing technologies, an increasingly clear picture is emerging of the vast genomic diversity of parasites from different geographic areas. This diversity is distributed throughout the genome and includes most of the vaccine candidates tested so far, playing an important role in the low efficacy achieved. Genomics is a powerful tool to search for genes that comply with the most desirable attributes of vaccine targets, allowing us to evaluate function, immunogenicity and also diversity in the worldwide parasite populations. Even predicting how this diversity might evolve and spread in the future becomes possible, and can inform novel vaccine efforts.
Diversity and Composition of the Skin, Blood and Gut Microbiome in Rosacea—A Systematic Review of the Literature
Authors: Tutka Klaudia , Żychowska Magdalena , Reich Adam
Published: November 2020

Rosacea is a chronic inflammatory skin disorder of a not fully understood pathophysiology. Microbial factors, although not precisely characterized, are speculated to contribute to the development of the condition. The aim of the current review was to summarize the rosacea-associated alterations in the skin, blood, and gut microbiome, investigated using culture-independent, metagenomic techniques. A systematic review of the PubMed, Web of Science, and Scopus databases was performed, according to PRISMA (preferred reporting items for systematic review and meta-analyses) guidelines. Nine out of 185 papers were eligible for analysis. Skin microbiome was investigated in six studies, and in a total number of 115 rosacea patients. Blood microbiome was the subject of one piece of research, conducted in 10 patients with rosacea, and gut microbiome was studied in two papers, and in a total of 23 rosacea subjects. Although all of the studies showed significant alterations in the composition of the skin, blood, or gut microbiome in rosacea, the results were highly inconsistent, or even, in some cases, contradictory. Major limitations included the low number of participants, and different study populations (mainly Asians). Further studies are needed in order to reliably analyze the composition of microbiota in rosacea, and the potential application of microbiome modifications for the treatment of this dermatosis.
Design of Anticancer 2,4-Diaminopyrimidines as Novel Anoctamin 1 (ANO1) Ion Channel Blockers
Authors: Kim Taewoo , Cho Sinyoung , Oh Haejun , Hur Joonseong , Kim Haedong , Choi Young-Ho , Jeon Seongho , Yang Young Duk , Kim Seok-Ho
Published: November 2020

Pyrimidine is a privileged scaffold in many synthetic compounds exhibiting diverse pharmacological activities, and is used for therapeutic applications in a broad spectrum of human diseases. In this study, we prepared a small set of pyrimidine libraries based on the structure of two hit compounds that were identified through the screening of an in-house library in order to identify an inhibitor of anoctamin 1 (ANO1). ANO1 is amplified in various types of human malignant tumors, such as head and neck, parathyroid, and gastrointestinal stromal tumors, as well as in breast, lung, and prostate cancers. After initial screening and further structure optimization, we identified Aa3 as a dose-dependent ANO1 blocker. This compound exhibited more potent anti-cancer activity in the NCI-H460 cell line, expressing high levels of ANO1 compared with that in A549 cells that express low levels of ANO1. Our results open a new direction for the development of small-molecule ANO1 blockers composed of a pyrimidine scaffold and a nitrogen-containing heterocyclic moiety, with drug-like properties.