bluuebunny/crossref_metadata_2025 huggingface dataset:
A Nonlinear Approach to Quantifying Investor Fear in Stock Markets of BRIC
Authors: Asafo-Adjei Emmanuel , Bossman Ahmed , Boateng Ebenezer , Owusu Junior Peterson , Idun Anthony Adu-Asare , Agyei Samuel K. , Adam Anokye Mohammed
Published: August 2022

The information flow between BRIC and relevant volatilities constitutes a complex network, which needs comprehensive analysis. We provide a rigorous investigation of information flow among stock markets of BRIC and the US VIX in a frequency-domain paradigm. Henceforward, the variation mode decomposition-based entropy approach is employed for the examination of diverse investment horizons and market conditions. First, we find that under stressed market conditions (lower quantiles), significant negative information flow exists between the BRIC constituents and the BRIC composite index. Also, under benign market conditions, we reveal similar dynamics as found at the lower quantiles, which enhances diversification. However, during market booms, we document more positive information flow between the assets and relevant to the redeployment of portfolios. Second, at low probability events representing market stress, we document potential negative information flow amid the stock markets and the US VIX for most investment horizons. Notwithstanding, the US VIX has the potential of transmitting positive information to the stock markets. However, at high market performance, we find more positive information flow amid the BRIC markets and VIX, generally implying long-term efficiency. Investors, portfolio managers, risk managers, and policy-makers should be wary of the heterogeneous and adaptive behaviour of BRIC stock markets with the VIX.
Identification of Potential miRNA-mRNA Regulatory Network in the Development of Oral Cancer
Authors: Yang Yun , Xin Xin , Xu Ting
Published: August 2022

Background. Oral cancer is a difficult question in modern medical system, and there are few effective strategies to completely heal these diseases. This research investigated the miRNA-mRNA network in oral cancer development via bioinformatics excavation. Methods. GSE28100 and GSE23558 in the GEO database were selected for bioinformatics analysis. The datasets were analyzed with GEO2R to obtain the related matrix files. The hot plot and heatmap of the matrix files were drawn with R language. The MiRDIP database was applied to predict and screen the targets of miRNAs. The DEGs in the matrix files were analyzed with the DAVID database and visualized with R language for enrichment analysis. The PPI-network of the DEGs was established with the STRING database and Cytoscape. Besides, the miRNA-mRNA was visualized by Cytoscape. Results. 35 genes were identified as the DEGs in GES28100. 1651 genes were identified as the DEGs in GSE23558. 143 common genes in the targets of miRNAs in GSE28100 and the DEGs in GSE the targets of DEGs in GES28100 and common genes were enriched in the PI3K/AKT pathway, MAPK pathway, etc. The DEGs in GSE28100 and GSE23558 were involved in the regulations of transcription from RNA polymerase II promoter and DNA transcription. The DEGs in GSE28100 and GSE23558 were established with the miRNA-mRNA network. Conclusion. This research identified miR-15b-5p, miR-199a-3p, miR-21-5p, miR-424-5p, and miR-454-3p as the biomarker of oral cancer and established the miRNA-mRNA network in oral progression.
Prenatal Diagnosis and Fetal Outcome with Mosaic Genome-Wide Uniparental Disomy
Authors: Jawahir-Schonauer Jessica , Norman Alexa , Mbanugo Chineze , Yu Grace , Rowsey Ross A. , MacDonald Erica , Romero Vivian C.
Published: August 2022

Introduction: While non-mosaic genome-wide paternal uniparental disomy (patUPD) is consistent with complete hydatidiform mole, the prenatal presentation of mosaic genome-wide patUPD is not well defined. This report adds another case to the small cohort of patients with the rare genetic disorder of mosaic genome-wide patUPD and provides one of the few examples of a prenatal presentation of this disease. We discuss ultrasound findings and prenatal analysis to review predominant genetic and clinical features associated with mosaic genome-wide patUPD. Case Presentation: A 30-year-old gravida 1 para 0 woman was referred at 10 weeks gestation due to an abnormal first-trimester ultrasound suggesting a partial molar pregnancy. The patient undertook genetic counseling and reviewed possible genetic etiologies and testing options. Karyotype analysis demonstrated a female fetus (46, XX). The BWS methylation pattern suggested the absence of maternally derived copies of IC1 (H19) and IC2 (LIT1) critical regions, which could result from patUPD of chromosome 11. CMA of cultured amniocytes was significant for arr(1–22,X)x2 hmz, consistent with genome-wide absence of heterozygosity (shown in Fig. 3). Discussion/Conclusion: This case report is intended to add to the limited knowledge regarding prenatal diagnosis of mosaic genome-wide patUPD by highlighting the ultrasound findings, the genetic testing performed, and fetal outcome. The fetal karyotype was normal. CMA was consistent with a molecular diagnosis of GWUPD. Low-level mosaicism in our sample was inferred given the clinical presentation of a developing fetus. Methylation studies were consistent with a diagnosis of BWS. The diagnosis of genome-wide patUPD using CMA provides further knowledge of UPD and its functional relevance. In a prenatal setting, a CMA profile without heterozygosity is typical of a complete molar pregnancy. However, in the presence of a fetus, it likely represents mosaic GWUPD, a rare condition that is usually of paternal origin.
Symptomatic Dermal Inclusion Cysts in Infants following Fetal Surgery for Myelomeningocele: Report of Two Cases and Review of the Literature
Authors: Best Benjamin J. , Truong Huy Q. , Foy Andrew B.
Published: August 2022

Introduction: It remains unclear if fetal repair of myelomeningocele (MM) is associated with a greater risk of developing symptomatic dermal inclusion cysts (ICs) at the neural placode. We report two infants treated with fetal surgery who developed symptomatic IC at less than 1 year of age, and we discuss the current literature on symptomatic IC in children with MM. Case Presentation: Two infants underwent fetal MM repair at 24 weeks of gestational age. Case 1 was born at 30 weeks and had two revisions of the MM wound early in life. At 8 months of age, the patient presented with meningismus and imaging findings of an IC, which was resected at the time of presentation. At 3 years of age, this patient was found to have recurrence of the IC after presenting with worsening bladder function and underwent repeat debulking with no recurrence at 6 years of age. Case 2 was born at 32 weeks of gestational age with uncomplicated recovery. At 8 months of age, the patient presented with irritability and fullness at the lumbar repair site. Imaging showed a large IC with restricted diffusion and extension into the subcutaneous tissue; this was resected completely at the time of presentation, see intraoperative photographs. There has been no sign of recurrence at age of 15 months. Conclusions: Careful monitoring for IC in infancy in MM patients who have had fetal surgery is recommended.
Uma rede de afetos, apesar da distância
Authors: Santos Claudiana Gois dos
Published: August 2022

Este artigo tem como objetivo discutir como redes sociais como o Instagram, geralmente entendido como fator de procrastinação, podem ser utilizadas como plataforma de pesquisa e divulgação científica e literária no contexto do distanciamento social recomendado durante a pandemia de COVID-19. A utilização das redes sociais como ferramenta de estudo proporcionou um contato diversificado com métodos de pesquisa e escrita acadêmica, bem como perfis de escritores dos livros que compõem o corpus da minha pesquisa de doutorado. Para isso, destaco três perfis no Instagram: o perfil da antropóloga e professora da Universidade de Brasília Débora Diniz (@debora_d_diniz), o perfil da escritora e pesquisadora Tatiana Nascimento (@tatiananascivento) e o perfil da também escritora e pesquisadora Natalia Borges Polesso (@nataliaborgespolesso). O acesso semanal às aulas de Débora Diniz proporcionou um uso mais efetivo dos métodos de escrita e pesquisa. Além disso, as discussões sobre o processo de escrita e recepção das obras dos autores, narradas por eles mesmos, auxiliaram na reflexão sobre o corpus da tese. Assim, conclui-se que as redes sociais, pensadas para outros fins, podem revelar-se instrumentos benéficos para a investigação e escrita acadêmica.
A literatura cada vez mais digital: a presença de escritoras e iniciativas culturais no meio virtual durante o distanciamento social
Authors: Bento Oluwa Seyi Salles
Published: August 2022

Os quase dois anos de pandemia causaram profundas mudanças em nossas vidas pessoais e em sociedade. Descobrimos, inventamos e recuperamos formas de nos relacionar, comunicar, locomover pela cidade e também de produzir e apreciar literatura. As lives no Instagram, Facebook e Youtube, os vídeos-minuto postados nestas e em outras plataformas, os eventos completamente virtuais  - como lançamentos, saraus e rodas de conversa sobre literatura - são fenômenos que, apesar de não terem sido criados nos longos meses de distanciamento social que experienciamos, se tornaram ferramentas basilares para o compartilhamento e a publicização de trabalhos literários e editoriais. Este artigo, que é um recorte de comunicação oral apresentada no XX Encontro de Estudos Comparados de Literaturas de Língua Portuguesa, objetiva apresentar e examinar algumas experiências virtuais que, ao longo da pandemia de COVID-19, foram e seguem sendo possíveis na trajetória de escritoras e iniciativas culturais brasileiras, como Carol Dall Farra, Conceição Evaristo a FLUP (Festa Literária das Periferias).
O futuro está pronto, nós viveremos
Authors: Fradico Silmara Ramos
Published: August 2022

Este artigo apresenta uma breve introdução sobre a trajetória da literatura de ficção científica tradicional e sobre alguns conceitos de tempo conforme as culturas Iorubás para então trazer uma análise comparada sobre o uso destes conceitos e do Afrofuturismo nas histórias Angola Janga e Os três Esús e o Tempo.
96–8–3–2: Indícios de primazia lexical em introduções de artigos acadêmicos
Authors: Aragão Rodrigo Moura Lima de
Published: August 2022

Este artigo dirige-se à seguinte questão: expressões funcionalmente semelhantes comportam-se de maneira parecida em línguas e disciplinas acadêmicas diferentes? Com o uso de princípios da teoria da primazia lexical, foram analisadas ocorrências de expressões de transição de escopo em introduções de artigos acadêmicos em inglês, português e japonês de pediatria e administração. O corpus foi composto por 96 introduções de artigos publicados em oito periódicos acadêmicos. A análise enfocou três aspectos: posição das expressões nas introduções (coligações textuais), conteúdos subsequentes (associações de conteúdo) e funções gramaticais (coligações). Como resultado, observaram-se semelhanças e diferenças entre línguas e disciplinas. As expressões japonesas distinguem-se sobretudo por particularidades de conteúdo e gramática. As expressões de pediatria mostram-se mais fixas e acompanhadas de conteúdos menos variados; as de administração, menos fixas e acompanhadas de conteúdos mais diversificados. Os achados podem ser aplicados ao ensino de línguas para fins específicos e à tradução acadêmica.
Entrevista com Sophie Moirand: Análise de discursos comparativa e relações com o Brasil e a América Latina
Authors: Moirand Sophie , Ribeiro Camila Fernanda
Published: August 2022

Esta entrevista aconteceu em fevereiro de 2022 via videoconferência. O objetivo foi conhecer melhor o percurso acadêmico de Sophie Moirand, suas ligações com a América Latina e o desenvolvimento da Análise de Discursos Comparativa, abordagem da qual ela é uma das fundadoras. A linguista argumenta que a ótica comparativa pode ser considerada algo que se constrói metodologicamente de acordo com cada objeto de estudo, mais do que uma perspectiva cujas teorias e possibilidades já estão completamente fixadas. Com bom humor e extrema generosidade no compartilhamento de seus conhecimentos e experiências, ela transforma a entrevista em uma verdadeira conversa, em uma troca rica e interessante que começa antes mesmo de as perguntas serem feitas. Descobrimos, assim, logo no início, que seu percurso acadêmico começa na área do Francês Língua Estrangeira (FLE), que foi o responsável por seus primeiros contatos com a América Latina, além de ser o motor propulsor que levará a pesquisadora ao encontro da Análise do Discurso. Veremos, assim, o quanto seu percurso acadêmico entrelaça-se com suas jovens experiências profissionais, como o interesse precoce pelo discurso jornalístico/midiático em Argel, na Argélia. Observaremos também que a construção de um percurso acadêmico não era mais fácil no passado do que hoje.
State of the Science: The Relevance of Symptoms in Cardiovascular Disease and Research: A Scientific Statement From the American Heart Association
Authors: Jurgens Corrine Y. , Lee Christopher S. , Aycock Dawn M. , Masterson Creber Ruth , Denfeld Quin E. , DeVon Holli A. , Evers Linda R. , Jung Miyeon , Pucciarelli Gianluca , Streur Megan M. , Konstam Marvin A. ,   on behalf of the American Heart Association Council on Cardiovascular and Stroke Nursing; Council on Hypertension; and Stroke Council
Published: August 2022

Symptoms of cardiovascular disease drive health care use and are a major contributor to quality of life. Symptoms are of fundamental significance not only to the diagnosis of cardiovascular disease and appraisal of response to medical therapy but also directly to patients’ daily lives. The primary purpose of this scientific statement is to present the state of the science and relevance of symptoms associated with cardiovascular disease. Symptoms as patient-reported outcomes are reviewed in terms of the genesis, manifestation, and similarities or differences between diagnoses. Specifically, symptoms associated with acute coronary syndrome, heart failure, valvular disorders, stroke, rhythm disorders, and peripheral vascular disease are reviewed. Secondary aims include (1) describing symptom measurement methods in research and application in clinical practice and (2) describing the importance of cardiovascular disease symptoms in terms of clinical events and other patient-reported outcomes as applicable.
Disparities in Reperfusion Therapy and Time Delays Among Patients With Ischemic Stroke and a History of Mental Illness
Authors: Mackenhauer Julie , Frischknecht Christensen Erika , Andersen Grethe , Mainz Jan , Johnsen Søren Paaske
Published: August 2022

Background:

Comorbid mental illness may delay recognition of stroke symptoms and reduce the chance of reperfusion therapy. The aim of this study was to compare the use of reperfusion therapy and treatment delays in patients with or without a history of mental illness.

Methods:

A nationwide registry-based cohort study of patients with ischemic stroke. We combined data from the Danish Stroke Registry and the Danish Quality Database for Prehospital Emergency Medical Services from 2016 to 2017 containing information on reperfusion therapy (thrombolysis or thrombectomy) and relevant time stamps. Patients were categorized according to the severity of their mental health history (how recent and severity, for example, hospital- versus primary-based care).

Results:

A total of 19 592 admissions were included (18% had a minor, 3% had a moderate, and 3% had a history of major mental illness). Significant differences were found regarding age, comorbidity, and socioeconomic factors. Reperfusion therapy was used in 17% of patients.

Patients

with a history of mental illness were less likely to receive reperfusion therapy: risk ratios with 95% CI were 0.79 (0.72–0.86) for minor, 0.85 (0.72–0.99) for moderate, and 0.63 (0.51–0.77) for patients with a history major mental illness, respectively. Total prehospital delay was longer for patients with a history of major mental illness compared to patients with no history of mental illness, especially when no call had been made to the emergency medical service. The median times from symptom onset to hospital arrival was 811 minutes (197–2845) and 115 minutes (41–188), respectively. We found no differences regarding door-to-needle time, response time, on-scene time, transport time, nor in time-to-imaging among patients arriving within 4 hours from symptoms onset between patients with and without a history of mental illness.

Conclusions:

Almost one-quarter of patients with ischemic stroke had a history of mental illness. Regardless of severity of mental illness, these patients were less likely to receive reperfusion therapy. Longer delays from symptom onset to hospital arrival contributed to the patients’ risk of not being eligible for reperfusion therapy.
Two new species and a new record of eriophyoid mites (Acari: Eriophyodea) from Guangxi, China
Authors: LV AN-KANG , HE WEI-QIAOCHU , GAO XIAN-LIANGYU , TAN MENG-CHAO , WANG GUO-QUAN
Published: August 2022

Two new species of eriophyoid mites are described and illustrated from Guangxi, China: Acaphylla quercus sp. nov. collected on Quercus glauca Thunb. (Fagaceae), Rhyncaphytoptus miliusius sp. nov. collected on Miliusa sinensis Finet & Gagnep. (Annonaceae). We further report Cecidodectes euzonus Nalepa, 1917, collected on Trema tomentosa (Roxb.) H.Hara (Cannabaceae), for the first time from China. All the species are vagrants on lower leaf surface causing no apparent damage to their host plants.
Molecular genetics of Simulium (Wilhelmia) equinum (Linnaeus) (Diptera: Simuliidae) from West Siberia
Authors: VAULIN OLEG V. , PETROZHITSKAYA LIUDMILA V. , NOVGORODOVA TATIANA A.
Published: August 2022

The medico-parasitological importance of bloodsucking black flies is high. To assess potential epidemiological risks and develop biological control programs for these bloodsucking Diptera, it is necessary to be informed of their diversity, community structure and the most common and dominant species. The degree of homogeneity of molecular genetic characteristics of one of the most widespread black fly species Simulium (W.) equinum (Linnaeus) in different parts of its range was investigated. The features of S. equinum from West Siberia where the species has become an abundant ecosystem component in recent years have been revealed. Analysis of species-diagnostic DNA-fragments (COI, ITS2 and D2 28S) in the specimens studied from this region, from the Novosibirsk Oblast (Russia) and data from the DNA database revealed four distinct branches tentatively referred to as West-European, East-European, Finland-Turkish, and Novosibirsk. Specimens from the Novosibirsk Branch were closer to the Finland-Turkish branch. We simulated restriction patterns of the standard COI fragment for all the branches. Substantial level of differences among the branches (4.1–14.8 % by COI sequences) raised the issue of their species status. A further study of S. equinum from West Siberia may clarify the ecological and medico-parasitological features of the Novosibirsk Branch of the species.
A new species of the diogenid hermit crab genus Pseudopaguristes McLaughlin, 2002 from the Northern Mariana Islands, Micronesia
Authors: KOMAI TOMOYUKI , MILLER ALLISON K. , MALAY MARIA CELIA D.
Published: August 2022

The diogenid hermit crab genus Pseudopaguristes McLaughlin, 2002 is currently represented by 15 species worldwide. In this study, we describe a new species of the genus, P. flavioculus, based on a unique male holotype from off Sarigan, Northern Mariana Islands, Micronesia, at depth of 131–133 m. The new species appears similar to the type species of the genus, P. janetkae McLaughlin, 2002, but the lack of conspicuous spines on the dorsal face of the right cheliped carpus, the possession of a median row of tiny spines on the dorsal face of the left cheliped carpus, and different colour pattern of the cephalic appendages and the ambulatory legs distinguish P. flavioculus n. sp. from P. janetkae. Pseudopaguristes has been differentiated from the two allied genera, Paguristes Dana, 1851 and Areopaguristes Rahayu, 2012, by the possession of eight functional pairs of gills (versus 13 pairs in Paguristes and 12 pairs in Areopaguristes), but our examination of the new species and three other congeneric species available for study clarifies that the development of arthrobranchs on the maxillipeds 3 and chelipeds is rather variable in species currently assigned to Pseudopaguristes. Careful observation of the anterior arthrobranchs is recommended to avoid misinterpretation in the counts of gill numbers.
Treatment Activity of a New Cd(II)-Based Complex on Prostatitis by Inhibiting the AMP-Activated Protein Kinase Signaling Pathway
Authors: Ma Kehe
Published: August 2022

In this work, a novel Cd(II) metal–organic framework, namely, [Cd3(L)2(H2O)4]·(CH3CN)2·(H2O)2 (1) [H3L: H3L = 4,4′,4″-((benzene-1,3,5-tricarbonyl)tris(azanediyl))tribenzoic
acid] is synthesized using solvothermal reactions. Then, a series of instrumental analyses, such as single-X ray diffraction, PXRD, FTIR, and elemental analysis, is conducted to demonstrate the properties. The application value of the framework on prostatitis is assessed, and the related mechanism
is discussed. After compound treatment, the level of inflammatory cytokines in the plasma is performed using the enzyme linked immunosorbent assay (ELISA). Finally, real-time Reverse Transcription-Polymerase Chain Reaction (RT-PCR) is used in exploring AMPK signaling pathway activation in
the prostate tissues.
Action of Tofacitinib in a Rat Model of Synovitis
Authors: Wang MingJun , Wu Jian , Cao Jing , Zhou Erye , Yin Yufeng , Chang Xin , Cheng Tao
Published: August 2022

Purpose: To evaluate effects and mechanism of tofacitinib in treatment of rheumatoid arthritis (RA) model rats. Materials and Methods: Dividing 27 rats into 3 groups: NC (normal control), Model (RA model) and Tofacitinib (RA model rats treated with tofacitinib) groups.
Observation joint swelling and articular synovium pathology by HE staining, IL-1β, IL-6, IL-10 and TNF-α levels by ELISA assay, JAK2, STAT3 and NF-κB(p65) proteins by IHC and WB assay. Results: Compared with NC group, joint swelling, histopathological
score IL-1β, IL-6, IL-10 and TNF-α significantly deteriorated (P < 0.001, respectively); by IHC and WB assay, JAK2, STAT3 and NF-κB(p65) proteins expression were significantly up-regulation in joint synovial tissue in model group (P <
0.001, respectively). With tofacitinib supplement, joint swelling, histopathological score IL-1β, IL-6, IL-10 and TNF-α significantly improved (P < 0.001, respectively); by IHC and WB assay, JAK2, STAT3 and NF-κB(p65) proteins expression significantly
down-regulation in joint synovial tissue in Tofacitinib group (P < 0.001, respectively). Conclusion: Tofacitinib could improve RA via regulation JAK2/STAT3/NF-κB(p65) pathway in vivo study.
Impact of Long Non-Coding RNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 on the Susceptibility of High Glucose-Treated Cardiomyocytes to Hypoxia/Reoxygenation
Authors: Liu Rongchen , Gao Xiufang , Shi Haiming
Published: August 2022

To estimate the effect of lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) on susceptibility of high glucose (HG)-treated cardiomyocytes (CMs) to hypoxia/reoxygenation (H/R). Forty healthy rats were assigned to the control, diabetes mellitus (DM), DM + ischemia/reperfusion
injury (IRI) control, and DM IRI groups (n = 10 for each group). Rat CMs (H9C2) were subjected to HG and H/R treatments. LncRNA MALAT1 and cyclic-AMP responsive element modulator (CREM) mRNA levels were measured using quantitative polymerase chain reaction, and protein levels of CREM,
myeloid differentiation primary response protein 88 (MyD88), interleukin-1 receptor-associated kinase 1 (IRAK1), tumor necrosis factor receptor associated factor 6 (TRAF6), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) were quantified using western blotting.
Flow cytometry was applied for detection of apoptosis, and a cell viability analyzer determined the number of living cells. IRI induced myocardial apoptosis and elevated lncRNA MALAT1, CREM, and MyD88/IRAK1/TRAF6 levels in DM group mice. In H9C2 cells, HG treatment downregulated CREM expression,
which resulted in the downregulation of lncRNA MALAT1 expression, reducing the susceptibility to H/R, increasing cell viability and apoptosis, decreasing the activity of the MyD88/IRAK1/TRAF6 signaling pathway, and suppressing TNF-α and IL-6 expression. LncRNA MALAT1 regulates
the susceptibility of HG-treated CMs to H/R through the MyD88 signaling pathway.
Bone Marrow Mesenchymal Stem Cell (BMSC) Restrains the Angiogenesis in Melanoma Through Stromal-Derived-Factor-1/C-X-C Chemokine Receptor Type 4 (SDF-1/CXCR4)
Authors: Jiao Liyan , Yang Qingyan , Miao Guoying , Wang Youming , Yang Zhitang , Liu Xiaojuan
Published: August 2022

This study analyzes the effect of BMSC on restraining the angiogenesis in melanoma through inducing SDF-1/CXCR4 channel. 50 female naked rates were equally assigned into NC group, model group, BMSC group, agonist group and positive NC group randomly followed by analysis of pathological
changes, and the level of HIF-1, VEG, MVD, SDF-1 and CXCR4. Agonist group showed the highest level of HIF-1α and VEGF and MVD followed by, model group BMSC group, positive NC group and NC group with no different between BMSC group and positive NC group. SDF-1 and CXCR4 expression
was highest in agonist group, followed by that in model group, positive NC group, BMSC group and NC group without difference between model group and positive NC group. In conclusion, SDF-1/CXCR4 activity could be restrained by BMSC partly along with reduced level of HIF-1α and
VEGF. This is mainly related with restraining the SDF-1/CXCR4 channel, indicating that it could be adopted as a brand-new therapeutic target for treating melanoma.
Effect of Co-Cultured Bone Marrow Mesenchymal Stem Cells (BMSC) and Neuropilin 1 on the Migration of Endometrial Stromal Cells and Epithelial-Mesenchymal Transition in Adenomyosis
Authors: Liu Lixue , Wang Saren , Li Debang , Tuo Ya
Published: August 2022

Our study intends to assess the effect of co-cultured bone marrow mesenchymal stem cells (BMSC) and Neuropilin 1 on the migration of endometrial stromal cells and epithelial-mesenchymal transition in adenomyosis. qRT-PCR was utilized for measuring Neuropilin 1 level in adenomyosis tissues
and cells. Endometrial stromal cells were treated with BMSC, si-Neuropilin 1, and pc-DNANeuropilin followed by analysis of cell proliferation and migration, as well as the expression of epithelial-mesenchymal transition (EMT)-related proteins. Neuropilin 1 expression was significantly upregulated
in 77 adenomyosis patients. Neuropilin 1 expression showed a positive correlation with the diagnostic sensitivity of adenomyosis. BMSC+pc-DNA-Neuropilin 1 treatment significantly promoted cell viability, migration, and EMT which were reduced by BMSC+si-Neuropilin 1. In conclusion, our study
demonstrates that co-culture of BMSCs and Neuropilin 1 increases cell viability, migration, and EMT in adenomyosis.
Supramolecular Mn(III) Coordination Polymer with Treatment Activity on Diabetic Retinopathy
Authors: Yang Chun-Juan , Li Xing-Mei
Published: August 2022

A new Mn(III) coordination polymer formulated as [Mn2(L)2Cl2(DMF)2]n (1, H2L is 2,2′-bipyridyl-6,6′-dicarboxylic acid; DMF is N,N′-dimethylformamide) was solvothermally prepared using Mn(ClO4)2
· 6H2O, H2L, and (CH3)4NCl as raw materials. The assay of SCXRD for 1 suggests that it features a 0D isolated dinuclear [Mn2(L)2Cl2(DMF)2] structure that exists in P-1 with triclinic
system, and the cell parameters are a = 8.4593(2), b = 9.4675(5), c = 11.0147(6) Å, α = 86.023(3), β = 74.892(6), γ = 78.055(4)°, V = 833.11(7) Å3, and Z = 1. These isolated 0D dinuclear [Mn2(L)2Cl2(DMF)2]
motifs were extended into a 1D supramolecular chain via the connection of intermolecular C–H…Cl together with the C–H…O H bond. Furthermore, the novel CP’s treatment activity against the diabetic retinopathy was investigated, and the associated mechanism of
the new compound was discussed.
Early B Cytokine 1 Improves the Proliferation, Invasion and Migration of Ovarian Cancer Cells by Transcriptional Inhibition of the Expression of Cripto-1
Authors: Song Chunhong , Zhen Juan , Wang Ying , Zhang Longying
Published: August 2022

Ovarian cancer is a common malignant tumor in obstetrics and gynecology and its pathogenesis is complex. EBF1 expression is significantly decreased in ovarian cancer tissues, but its specific mechanism in ovarian cancer has not been studied. In this study, GEPIA website predicted the
expression of EBF1 in ovarian cancer tissues. Expression of EBF1 in ovarian cancer cells was detected by RT-qPCR and western blot. CCK-8 and clone formation assay were used to detect the cell proliferation level. Wound healing and Transwell assays detected the levels of cell invasion and migration.
Western blot was used to detect the expression of migration-related proteins. Cell transfection techniques were used to overexpress or reduce the expression levels of EBF1 and Cripto-1. Luciferase assay was used to detect the transcriptional activity of Cripto-1 promoter mutant in ovarian
cancer cells. ChIP assay was used to verify the combination of EBF1 and the E1 element of the Cripto-1 promoter. The results showed that the expression of EBF1 was down-regulated in all ovarian cancer cell lines. Overexpression of EBF1 can inhibit the proliferation, invasion and migration
of ovarian cancer cells, which is achieved by inhibiting the expression of Cripto-1. Overall, EBF1 improved the malignant progression of ovarian cancer cells by transcriptional inhibition of the expression of Cripto-1.
Treatment Activity of Ho(III)-Based Coordination Polymer on Liver Cancer by the Inhibition of Vascular Endothelial Growth Factor Signaling Pathway Activity
Authors: Zhang Meng , Lin Feng , Hong Wei-Wen , Zheng Shuang , Fu Jun-Hui
Published: August 2022

A new 0D dinuclear coordination polymer [Ho2(L)6(phen)2] (1) was hydrothermally synthesized based on HoCl3·6H2O, organic ligand HL = 3-hydroxybenzoic acid, and the auxiliary ligand phen = 1,10-phenanthroline (L−
is the fully deprotonated organic ligand), and full characterization of the structure was performed via the X-ray single-crystal diffraction data. Once this newly synthesized novel compound was achieved, the way it acted inside the liver cancer was examined, and the corresponding mechanism
was determined. First, the Cell Counting Kit-8 (CCK-8) method was conducted to analyze the compound activity after the treatment of liver cancer cells. In addition, real-time reverse-transcription polymerase chain reaction (RT-PCR) method was used to examine the in-cell vascular endothelial
growth factor (VEGF) signaling pathway activity. The molecular docking simulation showed that the carboxyl and phenol groups contained active binding receptor sites, indicating that Ho complex has excellent biological activity.
Study on the Function of miR-134 on Cognitive Function of Vascular Dementia (VD) Rats and Mechanism About Oxidative Stress and Autophagy and Cofilin 2 Level
Authors: Xia Haimiao , Wang Haipeng , Li Yue , Luo Ye , Liu Yuxiang , Wang Feng
Published: August 2022

To discuss the mechanism of miR-134 in improving cognitive function of VD rats through regulating the oxidative stress and autophagy and reducing the expression of Cofilin 2. VD rats was established. They were disposed with miR-134 antagonist. The cerebral regulatory capacity was observed
through ethology. The pathological change in CAI area of hippocampus and cerebral cortex was observed with HE staining method. The regulation of miR-134 targeting downstream was analyzed through bioinformatics. The presentation level of SOD, GSH, ROS and MDA was detected. The expression of
LC1/LC-3 and p62 was detected with Western Blot assay. There was visible activated microglial cells and gliocyte proliferation in VD rat’s model. The myelination was weakened. They were improved notably through the treatment with miR-134 antagonist. The expression of MDA and ROS could
be restrained by miR-134 antagonist through reducing the expression of Cofilin 2. The expression of SOD and GSH could be increased and oxidative stress could be reduced. The level of autophagy could be decreased. The cognitive function of VD rats could be improved by miR-134 antagonist through
regulating the oxidative stress and autophagy and reducing presentation of Cofilin 2.
Elevated Expression of miRNA-181a-5p in Placenta Tissue of Preeclampsia
Authors: Chen Dandan , Wang Ruiling , Zhang Hui , Pang Huayan , Zou Jiening , Chen Min , Zhao Caizhen , Xiong Li
Published: August 2022

This study intends to assess miR-181a-5p expression profile in preeclampsia (PE). PE tissues from 30 patients and 30 normal pregnancy patients were obtained to measure miR-181a-5p level by RTPCR. Cell was transfected with miR-181a-5p mimics followed by analysis of cell migration by
woundhealing assay, cell proliferation by CCK-8 assay as well as IGF2BP2 expression by RT-PCR and Bcl-2, Bax, caspase3, and Bcl-xl level by western blot. miR-181a-5p was significantly upregulated in PE patients. Mimics transfection significantly decreased cell migration and proliferation.
Interestingly, miR-181a-5p bound to IGF2BP2 3′UTR. Further, miR-181a-5p upregulation decreased IGF2BP2 expression dose-dependently, upregulated the mRNA expression of Bax, caspase3 and Bcl-xl and downregulated Bcl-2. IGF2BP2 upregulated Bcl-2 and downregulated Bax and caspase3, which
were reversed by miR-181a-5p. miRNA-181a-5p overexpression restrains placental trophoblast cells by down-regulation of IGF2BP2, thereby participating in PE pathogenesis.
Facility Size and Risk Factors for Mortality in Pediatric Trauma
Authors: Hairr Marsha , Tumin Dmitry , Greene Erika , Ledoux Matthew , Longshore Shannon
Published: August 2022

Background

Pediatric trauma outcomes can vary across facilities, yet evidence on the relationship between facility bed size and pediatric trauma outcomes has been mixed. We aimed to identify how facility bed size might modify the impact of patient-level risk factors on mortality in pediatric trauma. We hypothesized that patient-level risk factors would have a stronger association with mortality at smaller trauma centers, and a weaker association with mortality at larger centers.

Methods

We used deidentified data obtained from the 2017-2018 Trauma Quality Programs registry, including patients ages 0-18 years of age who were admitted to the hospital. The primary outcome was in-hospital mortality. Facility bed size was dichotomized as large (>600 beds) vs small/medium (≤600 beds). Sensitivity analyses used 200 and 400 beds as alternative cutoffs. Interaction between facility bed size and patient characteristics was assessed using unadjusted logistic regression, with statistically significant interactions entered in a final, fully adjusted model.

Results

The analysis included 171 810 patients (mean age 10 ± 5 years; 65%/35% male/female), including 28% treated in a large hospital and 1.2% who died during the hospitalization. Controlling for trauma center level (or subsetting to pediatric trauma centers only), larger bed size did not reduce mortality risk associated with patient characteristics such as injury mechanism, injury severity, or patient demographics.

Conclusions

Contrary to our hypothesis, greater facility bed size was not associated with reduced mortality risk associated with patient characteristics. Future studies are needed to identify hospital practices or characteristics that can attenuate the excess risk of known patient-level risk factors.
Physical Activity Counseling and Documentation by Pediatric Residents in Primary Care: Before and After Introduction of the FITT Principle
Authors: Grace Benjamin , Taylor Lucas Candice , Shetgiri Rashmi , Cardenas Krystal , Perez de la Garza Giselle , Pak Youngju , Yee Jennifer K.
Published: August 2022

Physician training on physical activity (PA) counseling in patient care is highly variable. The objective of this study was to improve PA counseling by pediatric residents through introduction of the four components of PA, called the FITT principle (frequency, intensity, time, type). Pediatric residents ( n=30) received lectures, curriculum content, and an electronic smart-phrase addressing PA in obesity, including the FITT principle. Surveys assessed resident attitudes, and chart reviews ( n = 423 over 16 months) identified evidence of PA counseling including FITT principle components preintervention and postintervention. Survey results showed positive attitudes and confidence regarding primary care provider roles in counseling on PA, with no differences postintervention. Chart reviews demonstrated increased documentation on PA frequency postintervention (pre 31.9% vs post 50.9%, P=.00006), but no significant changes in intensity, time or type. In conclusion, a focused PA curriculum promoted PA counseling by pediatric residents, with increased documentation of one component of the FITT principle.
The Roles of Gender and Employment Status in Abdication Decisions
Authors: Hall Kayla , Pazda Adam
Published: August 2022

Abdication—the act of giving up a choice to another person—has been shown to prompt reciprocal generosity (Kardas et al., 2018), but it is not clear whether men or women are more likely to engage in abdication. The present research utilized a gift card scenario adapted from Kardas et al. (2018) in two Amazon Mechanical Turk samples to explore whether women abdicated more frequently than men, whether men abdicated to women less frequently than to other men, and whether employment status predicts abdication. In experiment one, participants ( N = 520) were assigned to one of three conditions in which they had to either choose who received a hypothetical gift card themselves or abdicate the decision to a friend (sex not specified, male friend, or female friend). Chi-square analyses revealed no significant differences between participants who abdicated and those who allocated; men and women had similar abdication patterns; there was no significant difference in abdication as a function of friend gender for male or female participants. In experiment two, participants ( N = 707) were again assigned to one of the three conditions but were also asked their employment status. Chi-square analyses indicated that participants generally preferred to be allocators rather than abdicators; women showed a similar abdication pattern to men; there was no significant difference in abdication as a function of friend gender for male or female participants. Lastly, unemployed participants abdicated more frequently (70%) than their employed counterparts (42%), and this was especially likely for women. These results have implications for potential factors that influence abdication decisions.
Prevalence of Mitral Valve Prolapse in Keratoconus Patients
Authors: Sharif K W , Casey T A , Coltart J
Published: August 2022

Fifty patients with advanced degrees of keratoconus, requiring corneal transplantation, were sreened for mitral valve prolapse by two dimensional echocardiography. The overall prevalence of 58% was found to be statistically higher than the prevalence of 7% found in a group of age and sex-matched controls. It was also found to be higher than the previously reported prevalence of 38% in a group of keratoconus patients with similar age and sex match to our series. The findings of our study in conjunction with the histopathological and biochemical similarities between the two conditions strongly suggest that they may be different manifestations of similar defects in collagen metabolism.
Dental Enamel Growth, Perikymata and Hypoplasia in Ancient Tooth Crowns
Authors: Hillson S W
Published: August 2022

This paper describes the hypoplastic defects commonly seen on the surface of ancient human tooth crowns, excavated from archaeological sites, and presents a new method for estimating the ages at which these defects were initiated during life. The method is based upon examination of microscopic incremental structures on the enamel surface and it is possible also to apply it to reconstruction of the sequence and timing of dental crown development. The method of examination is non-destructive and allows full use to be made of the large numbers of complete, unworn dentitions which are found amongst archaeological remains.
Delay in Presentation after Myocardial Infarction
Authors: Heriot Alexander G , Brecker Stephen J , Coltart D John
Published: August 2022

Thrombolytic therapy reduces mortality in acute myocardial infarction (AMI), giving maximal benefit with early treatment. In the UK delayed presentation after AMI may reduce the advantages of thrombolysis. To assess this, 103 patients presenting with AMI to two London Hospitals were interviewed to determine the length and cause of delay from onset of chest pain to arrival at hospital.

Forty-nine per cent of patients took longer than 2 h to arrive at hospital, and 21% took longer than 4 h. Patients who contacted their general practitioner (GP) had a significantly prolonged time delay (160 mins; 65–730: median; range) compared to those who went directly to hospital by ambulance (82 mins; 15–395; P<0.0005), or on their own (90 min; 15–855; P<0.005). Patients calling their GP took a similar duration to decide to seek help [decision time (30 min versus 25 mins) P=NS], but significantly longer to reach hospital once the decision was made (110 min versus 56 min; P<0.0001), than those proceeding directly to hospital. Believing the pain was cardiac in origin significantly shortened decision time (15 min versus 45 min; P<0.05), as did knowledge of the existence of thrombolysis (15 min versus 50 min; P<0.05) and lack of prior cardiac symptoms (18 min versus 42 min; P<0.05).

Only 14% were aware of thrombolysis. Rank correlation confirmed that decision and total delay time were age independent. Delays of this magnitude may compromise the efficiency of thrombolysis. Education should encourage patients with chest pain to seek early attention and in urban areas to attend hospital directly.
The Influence of Diagnostic and Therapeutic Laparoscopy on Patients Presenting with an Acute Abdomen
Authors: Scott H J , Rosin R D
Published: August 2022

The role of laparoscopy in the management of patients presenting to one surgical firm with an acute abdomen is discussed. Sixty-seven laparoscopies have been performed over an 18 month period and it has altered the diagnosis in 19.4% of cases and the management in 13.4% of cases.

At laparoscopy the diagnosis of appendicitis was made in 37 patients (81% had attempted laparoscopic appendicectomies); pelvic inflammatory disease in 15 patients; torted fimbrial cyst in two patients; and free pus in the right lower peritoneum as a result of a perforated appendix was seen in two patients. Normal laparoscopy was performed in five patients and four patients who presented with a perforated duodenal ulcer had the diagnosis confirmed at laparoscopy, in three cases the perforation was oversewn laparoscopically. Two laparoscopies were performed on trauma patients; one stabbing and one blunt trauma to the right hypochondrium.

It has been demonstrated that diagnostic laparoscopy is a useful adjunct to the general surgeon's armamentarium. It is suggested that the skill of laparoscopy is passed on to junior trainee surgeons who can use this technique to help attain a diagnosis in patients presenting with an acute abdomen.
Cervical Dorsal Column Stimulation Relieves Pain of Brachial Plexus Avulsion
Authors: Bennett M I , Tai Y M A
Published: August 2022

Five patients with deafferentation pain due to brachial plexus avulsion injuries were treated with cervical dorsal column stimulation (DCS). This has produced a clinically useful improvement in pain relief and mood scores during a mean follow-up period of 13.5 months. No complications have been encountered and the advantages of DCS are discussed.
Susceptibility of Helicobacter Pylori to the Antibacterial Activity of Manuka Honey
Authors: Somal N Al , Coley K E , Molan P C , Hancock B M
Published: August 2022

Honey is a traditional remedy for dyspepsia, and is still used for this by some medical practitioners although there is no rational basis for its use. The finding that Helicobacter pylori is probably the causative agent in many cases of dyspepsia has raised the possibility that the therapeutic action of honey may be due to its antibacterial properties. Consequently, the sensitivity of Helicobacter pylori to honey was tested, using isolates from biopsies of gastric ulcers. It was found that all five isolates tested were sensitive to a 20% (v/v) solution of manuka honey in an agar well diffusion assay, but none showed sensitivity to a 40% solution of a honey in which the antibacterial activity was due primarily to its content of hydrogen peroxide. Assessment of the minimum inhibitory concentration by inclusion of manuka honey in the agar showed that all seven isolates tested had visible growth over the incubation period of 72 h prevented completely by the presence of 5% (v/v) honey.
Drug Misusers in Police Custody: A Prospective Survey
Authors: Payne-James J Jason , Dean Peter J , Keys Derek W
Published: August 2022

Approximately 11% of individuals seen by forensic medical examiners in police custody in London are drug misusers. This prospective survey using an anonymized structured questionnaire attempted to define some of the characteristics of this selected group of drug misusers. The study was undertaken in Metropolitan Police Service stations (London, UK) within the area covered by Group IV Forensic Medical Examiners. One hundred and fifty consecutive drug misusers in police custody were assessed.

Of these individuals 77% used heroin; 30% used both heroin and cocaine regularly; 72% were injecting drugs; 32% were being prescribed drugs (e.g. methadone) by general practitioners or drug agencies. Those individuals prescribed drugs spent a similar amount per day on illicit drugs as those who were not (£100.30 versus £106). Four per cent of individuals were HIV-positive; 25.7% were hepatitis-B positive. Only 9.7% were aware that prophylaxis for hepatitis-B was possible. Seventy-four per cent had served previous prison sentences and of those 82% had used class A controlled drugs whilst serving the sentence. It is concluded that drug misusers seen in police custody tend to be recidivists. It seems that some clear means of identifying and ensuring referral and attendance at an appropriate agency may be the only way to break the cycle of drug misuse, prison and further drug misuse in this highly (self) selected group of patients.
Audit on Myocardial Infarction in a District General Hospital: Is There Room for Improvement in Diagnostic Accuracy?
Authors: Wong Wilson , Jones Adam , Goodwin Timothy J
Published: August 2022

The proportion of patients with an acute myocardial infarction (MI) whose diagnosis was missed on admission was accessed. The admitting consultants were then tested to see if they could diagnose the patients correctly when they were shown the admission histories and electrocardiograms (ECGs).

Twenty-six per cent of patients with a final diagnosis of MI were not correctly diagnosed on admission. Fifty-one per cent of all patients did not receive thrombolytic therapy (TT) mainly because the diagnosis was not made on admission. A smaller proportion of these patients were admitted to the coronary care unit (CCU).

The consultants only correctly diagnosed an average of 7.3 of the 20 patients who were mis-diagnosed on admission and would have prescribed TT to an average of 4.3 of these 20 patients.

A significant proportion of patients had a diagnosis of MI missed on admission and therefore did not receive TT. Therefore, another aid to diagnosis such as serum creatinine phosphokinase (CPK) measurement should be available as an emergency test.