bluuebunny/crossref_metadata_2025 huggingface dataset:
Utility of FebriDx in early identification of possible COVID19 infection
Authors: Karim Nawazish , Ashraf Muhammad Zubair , Naeem Muhammad , Anwar Tahir , Aung Hnin , Mallik Srikumar , Avraam Eleni , Kiran Sidra , Bandapaati Sareesh , Khan Faisal , Tsaknis Georgios , Reddy Raja V.
Published: May 2020

Background Reliable differentiation between uncomplicated and self-limiting acute respiratory tract infections (ARIs) and more severe bacterial respiratory tract infections remains challenging, due to the non-specific clinical manifestations in both systemic bacterial or viral infections. The current COVID-19 pandemic is putting extraordinary strain on healthcare resources. To date, molecular testing is available but has a long turnaround time and therefore cannot provide results at the point-of-care, leading to a delay in results thereby exposing patients to cross-infection and delay in diagnosis (1-3). Methods We prospectively evaluated the utility of FebriDx®, a point-of-care fingerstick blood test that can differentiate viral from bacterial ARIs through simultaneous detection of both Myxovirus-resistance protein A (MxA) and C-reactive protein (CRP), in rapidly determining viral cases requiring immediate isolation and confirmatory molecular testing, from non-infectious patients or bacterial infections that require antibacterial therapy.Results 75 consecutive patients were assessed and 48 eligible cases were tested with FebriDx®. Overall, 35 patients had FebriDx® test viral positive. All 35 patients had either positive rt-PCR (n=30) for COVID-19 or clinical picture highly suggestive of COVID-19 infection (PPV of 100% in a pandemic situation)[AB1] . In the 13 cases it was viral negative, rRT-PCR was also negative in all cases. In one case of LRTI, it was not possible to determine the exact cause of infection and a viral infection couldn’t be excluded. Including this patient, the NPV was 12/13 (92%) exceeding the NPV of rRt-PCR at 71% (12/17). Sensitivity was conservatively calculated at 97% (35/36) compared to 85.7% (30[RS2] /35) for rRt-PCR. Similarly the specificity of both FebriDx®and rRt-PCR was 100% (12/12).Conclusions In the current COVID-19, FebriDx® shows potential as a reliable POC test and a proxy marker of COVID-19 infection amongst inpatients in a secondary care setting. [AB1]35/35 equates to a sensitivity and specificity of 100% for COVID, would you be willing to say that instead of ‘near 100% ppv)? [RS2]I believe PCR was 85.7% (30/35), because PCR only detects the COVID cases
Fruit ripening-associated leucylaminopeptidase with cysteinylglycine dipeptidase activity from durian suggests its involvement in glutathione recycling
Authors: Panpetch Pawinee , Sirikantaramas Supaart
Published: May 2020

BackgroundDurian (Durio zibethinus M.) is a highly popular fruit in Thailand and several other Southeast Asian countries. It is abundant in essential nutrients and several sulphur-containing compounds such as glutathione (GSH) and γ-glutamylcysteine (γ-EC). Cysteinylglycine (Cys-Gly) generated by GSH catabolism is also found in durian fruit pulp. Cysteine (Cys) is a precursor of the sulphur-containing volatiles produced during durian fruit ripening and accounting for the strong odour and flavour of the ripe fruit. However, the genes encoding plant Cys-Gly dipeptidases have seldom been identified. Our aim was to analyse the Cys-Gly peptidase activity of leucylaminopeptidase (LAP) in durian fruit pulp. ResultsDzLAP1 and DzLAP2 were identified, which the former was highly expressed in the fruit pulp. DzLAP1 expression at various ripening stages and in response to ethephon/1-MCP treatments suggested that DzLAP1 is active and important at the early stages of fruit ripening. Biochemical characterisation showed that DzLAP1 is a metalloenzyme activated by Mg2+ or Mn2+, is approximately 63 kDa in size, and has an optimal alkaline pH of 9.5 which is typical of LAPs. Kinetic studies revealed that DzLAP1 had a Km of 1.62 mM for its preferred substrate Cys-Gly. DzLAP1-GFP was localised to the cytosol and targeted to the plastids. ConclusionsDzLAP1 has Cys-Gly dipeptidase activity in the γ-glutamyl cycle. The present study discloses that the LAPs identified in durian fruit pulp are implicated in the high sulphur-containing compound levels observed at full ripening.
Changes in body composition with a hypocaloric high-protein diet combined with sedentary, moderate and high-intense physical activity in overweight or obese males: a 6-month randomized controlled trial
Authors: Hernández-Reyes Alberto , Vidal A. , Cámara-Martos F. , Molina-Luque R. , Moreno-Rojas R.
Published: May 2020

Objectives

Although the effectiveness of high-intensity training has been widely recognized as positive in athletes, its application in a clinical context is not well established. There is enough evidence to confirm that high-intensity routines are safe and that at-risk populations, such as for overweight and obese subjects, can help from these weight loss treatments. The purpose of this study was to lead a trial to test whether manipulation of physical activity in extension to high protein diet intake would induce changes in total weight and body composition.
Methods

Sixty-eight healthy male volunteers were randomly assigned to one of the experimental groups: a control group with a low-level prescription of physical activity (1–4 METs), moderate physical activity group that performed 10.000 steps walking per day (5–8 METs) and intense physical activity group that trained exercises by at least 70% of VO2max three times a week (> 8 METs). All subjects followed a high-protein diet designed with a reduction of 500 kcal/day. Nutritional counseling was provided throughout the study period to help ensure dietary adherence. This intervention was delivered over twenty-four sessions as weekly one-on-one, face-to-face 30-min consultations.
Results

A significant reduction in body fat percentage was observed in the intense physical activity group (-20.9 ± 8.8 (CI95%: -25.6 – -16.2), p < 0.001). The change in muscle mass did not produce any significant differences between groups (p > 0.05). Concerning weight, a notable weight loss was found in those subjects who performed moderate or intense physical exercise, vs. those who did light exercise, (p < 0.001) and (p < 0.003), respectively.
Conclusion

our data strongly suggest that resistance training should be included, whenever possible, in any dietary treatment for improving the body composition of men with overweight or obesity.
Evaluation of the environmental impact of two types of food in intensive farming of rainbow trout fry (Onchoryncus mykiss. Walbaum, 1792).
Authors: abba El Hassan , Dahak Oumaima , Ainane Tarik , Yaacoubi Adnane El
Published: May 2020

The environmental impact of two types of food distributed during the first phase of intensive breeding of rainbow trout (Oncorhynchus mykiss) fry at the station of the National Center for Hydrobiology and Pisciculture constitutes the main objective of this study. the method used for the evaluation of the impact of these two foods is the mass balance method based on the calculation of the quantities of nitrogen and phosphorus excreted according to the quantities of food ingested and the composition of the carcasses. The results obtained show that the weight growth of fry and the rate of nitrogen and phosphorus rejection during the experimental period are very different and vary depending on the type of food received.
Inhibiting CBX4 efficiently suppress YAP nucleus translocation in hepatocellular carcinoma cells to protect against sorafenib resistance
Authors: zhao wei , Ma BO , Tian Zhihua , Han Haibo , Tang Jintian , Dong Bin , An Guo , Cao Baoshan , Wang Boqing
Published: May 2020

Background

The discovery of biomarkers that predict the response to sorafenib and increase the efficiency of drug therapy represents a clinical challenge. This study aimed to investigate the possible role of inhibiting CBX4 to deregulate of CSCs and to evaluate the contribution of these molecules to sorafenib resistance in advanced HCC.
Methods

HCC cell lines and a xenograft mouse model with resistance to sorafenib were employed to analyze the effects of miR424 on CSC characteristics. RNA expression was analyzed by RT-PCR and next-generation sequencing in a cohort of 106 HCC cancer patients and sorafenib-resistant (SR) cell lines, respectively, to validate the key microRNAs and targets in the network. CBX4 expression was determined in HCC cells to examine YAP1-mediated stem-cell-like activation and its association with sorafenib resistance.
Results

MicroRNA and mRNA profiles of SR cell lines identified miR424 and its direct target CBX4 as significantly associated with stem-cell-like properties, poor survival and clinical characteristics. Functional experiments demonstrated that miR424 suppressed CBX4, and CBX4 induced nuclear translocation of YAP protein but was not associated with protein production. When YAP1 and CBX4 were modulated either alone or together in SR tumors with CA3 and UNC3866, respectively, tumorigenicity and stem-like properties were extremely inhibited, thus indicated that these compounds exerted a strong antitumor effect in vivo against SR HCC cells.
Conclusions

Our results revealed that blocking the crosstalk between CBX4 and YAP1 is critical in response to sorafenib resistance, and it could be a promising therapeutic strategy for patients with advanced HCC.
Comparison of the effect of the graft with double strands peroneus longus tendon and with the four strands hamstring tendon on reconstruction of the posterior cruciate ligament injury
Authors: Lu Yue , Xu Hua , Zhou Zhenyu , Fang Lei , Tao Ran
Published: May 2020

Background

This prospective study was to analyze the clinical effect of double-stranded long peroneal tendon and the four strands hamstring tendon in reconstruction of posterior cruciate ligament (PCL), and to investigate the effectiveness and safety of the two surgical approaches in reconstruction of PCL, so as to provide guidance for clinical treatment of PCL injury.
Methods

Total 48 patients with complete rupture of PCL treated in our hospital from July 2015 to February 2017 were selected and divided into the Group A (reconstructed with double strands peroneus longus tendon, 25 cases) and the Group B (four strands hamstring tendon, 23 cases) according to the different grafts.
Results

The intraoperative time for tendon extraction in the Group A (20.87 ± 2.07 min) was significantly shorter than that in the Group B (26.79 ± 2.32 min). Compared with pre-operation, the tibial posterior displacement of the two groups decreased significantly 24 months after operation, and IKDC score, Lysholm score and Tegner score increased significantly. Nevertheless, the IKDC score, Lysholm score, Tegner score, AOFAS score and Maryland score between the two groups before and after operation were no significant differences. Compared with the quadrupled hamstring tendon, the double-stranded peroneal longus tendon has the advantages of simple and safe tendon extraction.
Conclusions

Both surgical methods are effective for the treatment of PCL injury. When the four strands hamstring tendon is accidental (tendon rupture or tendon variation) or ineffective, the peroneal longus tendon will be a good choice.
Do standard neurological assessments tell us more than we realise?
Authors: Donnan Luke , Baker Emma , Horn Anna , Robinson Caroline
Published: May 2020

Background Neurological assessments are commonly performed by podiatrists as a screening tool for peripheral neuropathy, and to identify the risk of foot ulceration and amputation. Monofilament and tuning fork assessments are routinely used to assess peripheral sensation. Whilst these assessments are commonly used to monitor foot health, there is potential for neurological results to illuminate a broader and more holistic perspective of a person’s overall health status. Methods Recruitment of fifty participants (31 female, 19 male; 71.78±9.64 years) for foot health screening was associated with a foot health week promotional event at the Charles Sturt University Community Engagement and Wellness Centre. Under the guidance of registered podiatrists, fourth year podiatry students completed basic neurological assessments to determine each participant’s neurological status. Participants also completed a modified Foot Health Status Questionnaire (FHSQ). Age and monofilament results were analysed using Spearman’s rho, while correlations involving FHSQ data were identified using a Kruskal-Wallis test. Results For those participants who reported an excellent rating of their own health, there was a statistically significant relationship with adequate vibration sensation (p<0.01). A person’s ability to get up from a sitting position (p<0.01), lift and carry shopping (p=0.02) and climb a flight of stairs (p=0.03), was significantly correlated with more sites detected using monofilament assessment. Discussion Significant correlations were observed between basic neurological assessments and a number of activities of daily living. While the findings reflect a correlational relationship, not causational, this still provides an opportunity for clinicians to view neurological assessment results more holistically. Whilst the immediate focus for a practitioner will be tissue viability, neurological findings may be useful to stimulate further discussion about a patient’s functional capacity by exploring issues beyond the presenting condition.
Barriers and facilitators to implementing Clinical Imaging Referral Guidelines among medical professionals: Protocol for a systematic mixed studies review
Authors: Kisembo Harriet Nalubega , Kinengyere Alison Annet , Sahal Abdirahaman Omar , Malumba Richard , Salaama Dina Husseiny , Kawooya Michael Grace
Published: May 2020

Background The past two decades have seen increasingly rapid advances in the field of diagnostic imaging technology. This has significantly contributed to the quality of medical care outcomes. However, a number of studies have found that 20%-50% of imaging requisitions are inappropriate and unjustified. This wastes the already meager resources and exposes patients to unnecessary radiation with increased risk of radiation induced cancers.Clinical Imaging Guidelines (CIGs) are evidence-based tools developed to support the imaging referrer’s decision-making process by choosing the most appropriate imaging investigation for a particular patient with a specific set of symptoms and signs. However, implementing CIGs has not been effective in several settings. Identifying factors that influence CIGs implementation could give an insight into the type of strategies to put in place before implementing CIGs This systematic review protocol is aimed at understanding barriers and facilitators that influence implementation of CIGs among medical professions. Review Methods The development of the systematic review protocol will follow Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines (PRISMA-P) (additional file 1) Key databases Pubmed (Medline) and Embase will be searched using relevant terms. The, experts in the field will be contacted for their opinion and references from included studies will also be searched Only literature written in the English language will be reviewed. All study designs will be included, and there will be no limit set by the year of publication. The criteria for inclusion will be those studies which document and discuss barriers and facilitators to implementing CIGs among medical professions. All identified studies will be screened by a single reviewer but Quality of the studies to be included and extraction of data will be independently performed by two reviewers. Any discrepancies will be resolved by consensus through discussion, with a 3rd reviewer as a tie breaker Pre-established categories of barriers and facilitators to implementing CIGs in practice from literature, will be used to assess content analysis Discussion The findings from this review will provide an insight and direction to the “champions” implementing adoption or adaption of CIGs, especially in Africa of what is ahead of them for proper planning The protocol has been registered at the International Prospective Register of Systematic Reviews (PROSPERO; registration number: CRD42020136372.
Comprehensive evaluation of risk factors for lymph node metastasis with papillary thyroid carcinoma in Southwest China patients
Authors: Dong Yan , Wang Dan , Luo Yisheng , Chen Ling , Bai Huili , Shen Yifan , Zhang Yangli , Chen Xueping , Su Xinliang , Zhao Jinqiu , Liu Huandong , Lu Jungao , Yao Zuoyi , Zhao Yajing , He Changlong , Li Xiaosong
Published: May 2020

Background: With the increasing incidences of papillary thyroid cancer(PTC), it is important to risk-stratify patients who may have more aggressive tumor biology. This study aimed to evaluate the risk factors for lymph node metastasis with PTC in Southwest China Patients which may provide a substantial reference for clinical diagnosis and treatment. Methods: 1045 PTCs (313 PTMC and 732 non-PTMC) between August 2016 and August 2019 were examined totally (including one Tibetan). BRAF V600E mutation was tested in all samples. The clinical data (gender, age, tumor location, sample source and pathological features) were retrospectively analyzed. Logistic regression analysis was performed to evaluate independent risk factors for LNM. Results: 181 out of 313 PTMC cases (57.8%), 145 out of 732 non-PTMC cases (19.8%) had BRAF V600E mutation, the Tibetan had a double mutation of BRAF L597Q and V600E in two separate lesions. In PTMC, significant difference in gender and sample source was found (BRAF V600E mutation vs. wild-type). In non-PTMC, significant difference in gender was found (BRAF V600E mutation vs. wild-type). The female (OR=1.952; 95% CI= 1.373-2.774; P= 0.00), age (31-59 years) and diameter of tumor ≤1cm (OR=3.273; 95% CI= 2.417-4.432; P=0.000) were significant independent predictors of LNM in all PTCs. In PTMC, the female (OR= 3.002; 95% CI= 1.654-5.446; P= 0.00) was a significant independent predictor of LNM. The tumor in left and right lobes simultaneously was an independent protective factor of LNM in each group (PTCs: OR=0.287; PTMC: OR=0.170; non-PTMC: OR=0.441, respectively). The BRAF V600E mutation rate of US-FNAC was much higher than FFPE in PTMC (P=0.018). Conclusions: Unlike previous research, our findings suggested that the female patients and diameter of tumor ≤1cm were risk factors for LNM and the BRAF V600E wild-type of PTMC might be more aggressive than others. Interestingly, the position of tumor in bilateral thyroid simultaneously was an independent protective factor for LNM. The US-FNA should be recommended for gene analysis (BRAF V600E) in PTMC. The BRAF L597Q mutation may be an independent aggressive factor in the Chinese Tibetan population. Hence, clinicians should consider an individualized treatment according to gene mutation, gender, age, tumor size and location of tumor in order to achieve a better therapeutic efficacy.
Identification of Potential Genes in Her2+ Breast Cancer by Co-expression Network Analysis
Authors: Li Xinyang , Wang Yukun , Wang Ziming , Yao Ge , Fan Jiahao , Liang Gaofeng , Wang Xinshuai
Published: May 2020

Background

Breast cancer (BC) is one of the most common malignancies in women all over the world. For patients with human epidermal growth factor receptor 2 positive (Her2+) BC, although the widespread use of anti-Her2 therapy has make for survival increased, improved survival has led to an increased demand for better management and diminish toxic side effects of anticancer treatments. This study aimed to identify the potential biomarkers associated Her2 + BC in order to improve the overall management of BC treatment.
Results

Our research downloaded GSE54140 gene expression datasets from the Gene Expression Omnibus data sets, and used weighted gene co-expression network analysis (WGCNA) to developed a scale-free gene co-expression network to explore the associations between gene sets and clinical features. A total of 60 modules were analyzed, and found that the skyblue3 module was significantly related to Her2 + BC. The function of 93 genes in the skyblue3 module was annotated by DAVID bioinformatics tool, and it was demonstrated that the function of the module was mainly related to nuclear-transcribed mRNA catabolic process, cytosol, and oxidoreductase activity. Based on the WGCNA and Cytoscape software analysis, 9 hub genes (PGAP3, PPP1R1B, PNMT, ERBB2, CISD3, CRKRS, TCAP, STARD3, and NEUROD2) were identified. The Human Protein Atlas database detected that the protein level of PGAP3, PPP1R1B, PNMT, ERBB2, CISD3, CRKRS, TCAP, and STARD3 in tumor tissues were different from normal tissues. And survival analysis shows that PGAP3, PNMT, ERBB2, TCAP, and STARD3 were negatively associated with the overall survival (P < 0.05).
Conclusions

In total, 9 candidate biomarkers were identified by comprehensive analysis, among which, the co-expansion of PGAP3, CRKRS, STARD3 and NEUROD2 related to ERBB2 may be associated with the occurrence of Her2 + BC. In addition, PPP1R1B, CRKRS and TCAP are related to drug resistance and adverse reactions in the treatment of Her2 + BC.
Novel Water-Soluble Photosensitizer for Photodynamic Inactivation of Gram-Positive Bacteria Using Nontoxic Dose of UV Light
Authors: Yang Zihuayuan , Qiao Ying , Li Junying , Wu Fu-Gen , Lin Fengming
Published: May 2020

Background Antimicrobial photodynamic therapy (APDT) is a promising alternative to traditional antibiotics for bacterial infections, which inactivates a broad spectrum of bacteria. However, it has some disadvantages including poor water solubility and easy aggregation of hydrophobic photosensitizers (PS), and poor tissue penetration and cytotoxicity when using UV as the light source, leading to undesired photodynamic therapy efficacy.Results In this study, we develop a novel water-soluble natural PS (sorbicillinoids) obtained by microbial fermentation using recombinant filamentous fungus Trichoderma reesei (T. reesei). Sorbicillinoids could effectively generate singlet oxygen (1O2) under ultraviolet (UV) light irradiation, and ultimately display photoinactivation activity on Gram-positive bacteria, but not Gram-negative ones. Staphylococcus aureus (S. aureus) treated with sorbicillinoids and UV light displayed high levels of intracellular reactive oxygen species (ROS), notable DNA photocleavage, and compromised cell semi-permeability without overt cell membrane disruption. Moreover, the dark toxicity, phototoxicity or hemolysis activity of sorbicillinoids is negligible, showing its excellent biocompatibility.Conclusion Sorbicillinoids obtained from T. reesei display photoinactivation activity on Gram-positive bacteria using nontoxic dose of UV light irradiation and have an excellent biocompatibility Therefore, sorbicillinoids, a type of secondary metabolite from fungus, has a promising future as a new PS for APDT.
Masticatory Function In Elderly Compared To Young Adults
Authors: Ramos Verônica , Silva Anderson , Picinato-Pirola Melissa
Published: May 2020

Background

The performance of masticatory function in the elderly tends to be reduced due to dental absences, reduction of tone and muscle strength and incoordination of the orofacial musculature.
Aims

To characterize the masticatory function of the elderly and to compare total amount of time, masticatory strokes and total mastication score among the elderly and young adults.
Methods

A total of 50 individuals participated, 25 elderly (mean age 66 years) and 25 young adults (mean age 22 years). The evaluation of mastication was performed by standardized filming of the usual mastication of a wheat flour biscuit. The masticatory type (alternated bilateral, simultaneous bilateral, preferential unilateral, chronic and anterior), masticatory score, total masticatory time and the total number of masticatory strokes were verified and compared between the elderly and young adults.
Results

The predominant masticatory pattern in the young adults was the alternated bilateral mastication (52%), while, in the elderly, the simultaneous bilateral mastication predominated (48%). The use of dental prostheses had a significant influence on the total mastication score; elderly presented greater masticatory time and greater amount of masticatory strokes; however, the total masticatory score was lower for this group.
Conclusions

The use of dental prosthesis has a significant influence on masticatory function. The elderly had a greater amount of time and masticatory strokes and a lower total mastication score.
Identification and characteristics of postnatal development-related long non-coding RNAs under microbiota dependent condition
Authors: Zheng Miaomiao , Zhang Binbin , Zhang Yidan , Sun Tingting , Hu Baozhong
Published: May 2020

BackgroundThe interplay of long-non coding RNAs (lncRNAs) and the intestinal microbiota may serve as an essential role in intestinal development and homeostasis. Microbiota could regulate a large numbers of lncRNAs expression in intestinal epithelial cells. However, the associations between lncRNAs and microbiota during early postnatal development stages are still need to understand. MethodsIn present study, the microbial effects on lncRNA of intestinal epithelial cells (IECs) during postnatal development stage were investigated. ResultsWe identified gut microbiota-specific lncRNAs in diverse postnatal development stages including week 1, week 4 and week 12/16 of mice. A large proportion of gut microbiota-specific lncRNAs only were differential expressed in a single postnatal development stage. Up- and down-regulated gut microbiota-specific lncRNAs both showed consistent expression pattern. We also constructed gut microbiota-specific lncRNAs and coding genes interacted co-expressed networks. Functional analysis indicated that gut microbiota-specific lncRNAs were associated with ABC transporters. ConclusionsIn summary, the present study characterizes the landscape of lncRNAs associated with gut microbiota in different postnatal development stages. It provide assistance for exploring the relationships among lncRNAs, gut microbiota and postnatal development stages.
Inhibiting roles of FOXA2 in hepatocellular carcinoma cell migration and invasion by transcriptionally suppressing microRNA-103a-3p and activating the GREM2/LATS2 axis
Authors: Ma Guangzhen , Chen Jirong , Wei Tiantian , Wang Jia , Chen Wenshan
Published: May 2020

Background Forkhead box A2 (FOXA2) is a transcriptional activator for liver-specific genes. Hepatocellular carcinoma (HCC) is a prevalent fetal malignancy across the globe. This work focused on the role of FOXA2 in HCC cell migration and invasion and the involving molecules. Methods FOXA2 expression in HCC tissues and cells was determined using RT-qPCR. Altered expression of FOXA2 was introduced to identify its role in HCC cell migration and invasion using Transwell assays. The potential target microRNA (miRNA) of FOXA2 was predicted via online prediction and validated through a ChIP assay, and the mRNA target of miRNA-103a-3p was predicted and confirmed through a luciferase assay. The roles of miR-103a-3p and GREM2 in HCC cell invasion and migration were determined, and the downstream molecules mediated by GREM2 were analyzed. Results FOXA2 and GREM2 were poorly expressed while miR-103a-3p was abundant in HCC tissues and cells. Overexpression of FOXA2 or GREM2 suppressed migration and invasion of HepG2 and SK-HEP-1 cells, while up-regulation of miR-103a-3p led to reverse trends. FOXA2 transcriptionally suppressed miR-103a-3p to increase GREM2 expression, and silencing of GREM2 partially blocked the inhibitory effects of FOXA2 on cell migration and invasion. GREM2 increased LATS2 activity and YAP phosphorylation and degradation. Conclusion This study evidenced that FOXA2 inhibits migration and invasion potentials of HCC cell lines through suppressing miR-103a-3p transcription. The following upregulation of GREM2 plays key roles in migration inhibition by promoting LATS2 activity and YAP phosphorylation. This study may offer new insights into HCC treatment.
Alcohol drinking as a mediator of the influence of smoking prevalence on second-hand smoke exposure in workplaces: A mediation analysis
Authors: LIN Haoxiang , Chang Chun , LIU Zhao
Published: May 2020

Objective: Both alcohol drinking and second-hand smoke (SHS) exposure have shown a strong association with smoking behaviour. This study uses mediation analysis to clarify the process underlying the relationship between smoking prevalence and SHS exposure and the extent to which this relationship is mediated by alcohol use in the workplace.Method: A total of 14195 employees from 81 companies in China participated in this survey. Mediation analysis was used to establish the mediation effect of alcohol drinking. Structural equation modelling（SEM） was used to evaluate the role of alcohol drinking when a smoke-free (SF) workplace policy was imposed.Results: For males, approximately 6.3% of the effects on SHS exposure were mediated through the channel of alcohol use. For females, this channel mediated 14.1% of the total effects. SF policy was negatively associated with smoking prevalence and SHS exposure. The indirect effect of the SF policy on reducing SHS exposure by controlling smoking behaviour was significant. For smokers, worksite smoking bans were negatively associated with the smoking amount and drinking behaviour, and the indirect effect of this policy on promoting smokers’ intention to quit by reducing the smoking amount and controlling drinking behaviour was significant.Conclusion: This study confirmed that alcohol drinking is among the channels that mediate the association between smoking prevalence and SHS exposure in workplaces. Our results also find a spillover effect of SF workplace policy and call for effective interventions for alcohol use, which may influence the outcomes of SF policy implementation.
Price behavior of Turmeric in Sangali APMC
Authors: KOLEKAR PALLAVI LALASAHEB , shinde reshma , Shinde Shital Pravin
Published: May 2020

Prices of Agricultural commodity fluctuates time to time, in Sangali turmeric is one of the major crop grown, the prices of Turmeric are inversely related with arrivals. After harvesting arrivals are more in AMPC And prices are less. Seasonal indices and growth rate are used for study purpose.
Disruption of the CCL5/RANTES-CCR5 Pathway Restores Immune Homeostasis and Reduces Plasma Viral Load in Critical COVID-19
Authors: Pattterson Bruce , Seetthamraju Harish , Dhody Kush , Corley Michael , Kazempour Kazem , Lalezari Jay , Pang Alina , Sugai Christopher , Francisco Edgar , Pise Amruta , Rodrigues Hallison , Ryou Matthew , Wu Helen , Webb Gabriela , Park Byung , Kelly Scott , Pourhassan Nader , Lelic Alena , Kdouh Lama , Herrera Monica , Hall Eric , Aklin Enver , Ndhlovu Lishomwa , Sacha Jonah
Published: May 2020

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), is now pandemic with nearly three million cases reported to date1. Although the majority of COVID-19 patients experience only mild or moderate symptoms, a subset will progress to severe disease with pneumonia and acute respiratory distress syndrome (ARDS) requiring mechanical ventilation2. Emerging results indicate a dysregulated immune response characterized by runaway inflammation, including cytokine release syndrome (CRS), as the major driver of pathology in severe COVID-193,4. With no treatments currently approved for COVID-19, therapeutics to prevent or treat the excessive inflammation in severe disease caused by SARS-CoV-2 infection are urgently needed. Here, in 10 terminally-ill, critical COVID-19 patients we report profound elevation of plasma IL-6 and CCL5 (RANTES), decreased CD8+ T cell levels, and SARS-CoV-2 plasma viremia. Following compassionate care treatment with the CCR5 blocking antibody leronlimab, we observed complete CCR5 receptor occupancy on macrophage and T cells, rapid reduction of plasma IL-6, restoration of the CD4/CD8 ratio, and a significant decrease in SARS-CoV-2 plasma viremia. Consistent with reduction of plasma IL-6, single-cell RNA-sequencing revealed declines in transcriptomic myeloid cell clusters expressing IL-6 and interferon-related genes. These results demonstrate a novel approach to resolving unchecked inflammation, restoring immunologic deficiencies, and reducing SARS-CoV-2 plasma viral load via disruption of the CCL5-CCR5 axis, and support randomized clinical trials to assess clinical efficacy of leronlimab-mediated inhibition of CCR5 for COVID-19.
Anosmia, a Hidden Sign for COVID-19? A Case Report and Literature Review
Authors: Christelle Karen , Zulkifli Maryam Mohd , Draman Nani
Published: May 2020

Background The coronavirus disease 2019 (COVID-19) is an ongoing viral pandemic that is actively affecting 210 countries worldwide, with a total of more than 1.5 million cases and 106 000 deaths. Symptoms associated with COVID-19 are mainly fever, cough, dyspnea and sore throat. The current indication for COVID-19 testing includes presence of these symptoms with a positive history of travel to affected countries or contact with COVID-19 patients. Anosmia has been recently reported anecdotally over the past weeks as an emerging symptom of the COVID-19 but has yet to gain recognition as a symptom for COVID-19 by the World Health Organization (WHO) and Centre for Disease Control and Prevention (CDC). This case report highlights a case of isolated sudden onset of anosmia as a presenting symptom of COVID-19 and relevant literature review supporting the incidence of anosmia in COVID-19. This is a first case report of anosmia in COVID-19 occurring in pregnancy.Case Presentation A 30-year-old pregnant lady at 11 weeks of gestation presented with sudden onset of anosmia for one day with no other accompanying symptoms. She had just recovered from a mild cold a day prior to the development of anosmia. She had a history of travel by land to Singapore 14 days prior to onset of anosmia. There was no known close contact with a COVID-19 patient or attended any mass gatherings prior to development of her symptom. She underwent nasopharyngeal and oropharyngeal swab sampling which was then tested using reverse transcription polymerase chain reaction (RT-PCR) method and confirmed infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).Conclusion Clinicians should be aware regarding anosmia as a presenting symptom of COVID-19 especially in the presence of risk factors such as travel to affected countries and having close contact with COVID-19 positive patients. If testing is not done, these patients should be advised for home quarantine to reduce the risk of transmission. Healthcare workers must always adhere to infection control and prevention protocol as well as personal protective equipment.
Inhibition of papain-like protease PLpro blocks SARS-CoV-2 spread and promotes anti-viral immunity
Authors: Shin Donghyuk , Mukherjee Rukmini , Grewe Diana , Bojkova Denisa , Baek Kheewoong , Bhattacharya Anshu , Schulz Laura , Widera Marek , Mehdipour Ahmad Reza , Tascher Georg , Geurink Paul P. , Noort Gerbrand J. van der Heden van , Ovaa Huib , Knobeloch Klaus- Peter , Rajalingam Krishnaraj , Schulman Brenda A. , Cinatl Jindrich , Hummer Gerhard , Ciesek Sandra , Dikic Ivan
Published: May 2020

Main protease and papain-like protease (PLpro) are essential coronaviral enzymes required for polypeptide processing during viral maturation. PLpro additionally cleaves proteinous post-translational modifications from host proteins to evade anti-viral immune responses. Here, we provide biochemical, structural and functional characterizations of PLpro from SARS-CoV-2 (PLproCoV2) and reveal differences to that of SARS (PLproSARS) in controlling interferon (IFN) and NF-kB pathways. PLproCoV2 and PLproSARS share 83% sequence identity, yet they differ in their host substrate preferences: PLproCoV2 predominantly cleaves the ubiquitin-like protein ISG15 off from host proteins, while PLproSARS preferentially targets ubiquitin chains. The crystal structure of PLproCoV2 in complex with ISG15 explains the affinity and higher specificity through distinctive binding to ISG15’s unique amino-terminal ubiquitin-like domain, and enabled the identification of GRL-0617 as a non-covalent candidate inhibitor for PLproCoV2. In human cells, PLproCoV2 cleaves ISG15 from interferon responsive factor 3 (IRF3), blocks its nuclear translocation, and reduces type I interferon responses, whereas PLproSARS preferentially mediates deubiquitination of critical components of the NF-kB pathway. Pharmacological inhibition of PLproCoV2 blocks the virus-induced cytopathogenic effect upon infection with SARS-CoV-2, fosters the anti- viral interferon pathway and reduces viral release from infected cells. We propose that therapeutic targeting of PLproCoV2 can suppress SARS-CoV-2 infection and promote anti-viral immunity.
O DIREITO FUNDAMENTAL AO PLANEJAMENTO FAMILIAR E A NECESSIDADE DE REGULAMENTAÇÃO DA REPRODUÇÃO HUMANA ASSISTIDA
Authors: AMORIM João Vítor Lopes , AYLON Lislene Ledier
Published: May 2020

Os direitos fundamentais da pessoa humana são reconhecidos e garantidos pela Constituição Federal de 1988. Dentre eles, encontra-se o planejamento familiar, determinado no artigo 226, §7° da Carta Maior. O referido direito é estabelecido como livre escolha do casal, obrigando-se o Estado a propiciar os meios médicos e educacionais para que possa ser exercido, proibidas as práticas impeditivas por parte do poder público ou privado e suas respectivas instituições. Isto posto, como pode se comportar o planejamento familiar em vista do empecilho reprodutivo, nos casos de esterilidade ou infertilidade do casal? Com o grande aumento da busca das técnicas de reprodução assistida no território brasileiro devido à sua popularidade atual, não restam dúvidas de que questões jurídicas importantes são levantadas a todo momento. No entanto, em virtude da ausência de legislação sobre o assunto, a resolução desses conflitos torna-se complexa. Este artigo, portanto, tem o intuito de compreender os principais aspectos jurídicos do emprego das técnicas de reprodução assistida, recorridas quando há impossibilidade ou dificuldade do casal em procriar, visto que a ausência de uma regulamentação específica permite várias lacunas e interpretações.
A INCLUSÃO DO DEFICIENTE NA SOCIEDADE EDUCACIONAL: UMA ANÁLISE DO VIÉS PRINCIPIOLÓGICO DA EFICÁCIA HORIZONTAL DOS DIREITOS FUNDAMENTAIS APLICADA À RELAÇÃO DOS PARTICULARES DA EDUCAÇÃO
Authors: ARAUJO Larissa Janoni de , CARVALHO Roberta Dos Santos Pereira de
Published: May 2020

O objetivo principal do presente artigo empreendeu investigar os obstáculos de outrora e os ainda não sobrelevados pela Instituição de Ensino Regular Privada, no que diz respeito a inclusão da pessoa com deficiência no ambiente escolar privado, e, se essa mesma inclusão dentro do vínculo relacional particular tem sido amparada pela aplicação eficaz horizontal da norma pelos particulares da educação. Pautado em um estudo de caráter histórico sociológico, filosófico normativo e pedagógico, constata-se que a inclusão dessas pessoas depende, acima de tudo, do empenho conjunto do Estado aliado a Sociedade Educacional para que haja a operacionalização da norma fundamental também dentro do vínculo relacional particular.
A ALIENAÇÃO PARENTAL E A PERDA DO PODER FAMILIAR
Authors: PARANHOS Bruna Queiros , BERTONI Rosângela Aparecida Vilaça
Published: May 2020

Este artigo tem o objetivo de fazer uma revisão da monografia com o tema “A Alienação Parental e a Perda do Poder Familiar” no Brasil. A Alienação Parental é um fenômeno complexo que tem atingido significativamente grande parte das crianças da sociedade. Suas consequências apontam para questões de ordem psicológica, psiquiátrica e social. Têm-se apresentado teorias que partem de hipóteses e também algumas concepções que procuram identificar as possíveis causas da Alienação Parental.
NEGÓCIOS JURÍDICOS PROCESSUAIS APLICADOS AOS CONTRATOS DE LOCAÇÃO DE IMÓVEIS URBANOS
Authors: ARANTES Isabella Branquinho , MILAN Victor Hugo Polim
Published: May 2020

O presente trabalho disserta sobre a aplicação do negócio jurídico processual, previsto nos artigos 190 e 191 do Código de Processo Civil, aos contratos de locação de imóveis urbanos, buscando estabelecer algumas hipóteses em que é possível as partes convencionarem os seus direitos, ônus e deveres. De início, aborda a previsão do negócio jurídico processual no novo CPC, apontando os requisitos legais para a sua validade, encontrados tanto na legislação processual civil, quanto na legislação civil. Apresenta, em complemento, alguns dos princípios processuais e contratuais mais importantes quando se trata de Processo e de Contrato, a fim de delimitar o âmbito de aplicação das convenções processuais no caso concreto. Desprende, por fim, a possibilidade ou não de determinadas transações contratuais, concluindo se capazes de produzir pleno efeito, no caso de validação pelo juiz, ou se defesas por colidirem com algum Princípio ou requisito legal. Para tanto, utiliza ampla pesquisa bibliográfica, unindo os ramos de direito constitucional, processual e civil a fim de balizar a atuação das partes e garantir que as normas de ordem públicas serão respeitadas.
DIREITO E FILOSOFIA: REFLEXÕES SOBRE A JUSTIÇA EM ARISTÓTELES
Authors: CARVALHO Eduardo Vieira , GERA Marcos Silvestre
Published: May 2020

O presente artigo tem como desígnio fornecer uma visão sintética e panorâmica a respeito da virtude da justiça no pensamento aristotélico. Nessa perspectiva, o artigo objetiva compreender a visão que o maior filósofo de toda a Grécia Antiga tinha sobre a virtude ora em tela. Dessa feita, o presente trabalho realiza uma triagem dentro do pensamento sobre o justo em Aristóteles no sentido de selecionar os temas mais pertinentes e merecedores de serem trabalhados com mais afinco e prestígio. O método utilizado para a realização do artigo consiste no bibliográfico, uma vez que o trabalho foi todo desenvolvido por meio da consulta a obras teóricas a respeito do tema.
NEGÓCIOS JURÍDICOS PROCESSUAIS: OS LIMITES À LIBERDADE DAS PARTES QUANTO AO OBJETO NEGOCIÁVEL NA DINÂMICA PROCESSUAL CIVIL
Authors: RODARTE Gabriela Taveira , CASTRO Fabiana Maria Martins Gomes de
Published: May 2020

A presente pesquisa aborda sobre o negócio jurídico processual, temática que ganhou destaque com o advento do Código de Processo Civil de 2015 pela inédita previsão no artigo 190. O instituto compreende a faculdade de as partes ajustarem o procedimento conforme as características singulares da causa, a fim de imprimir eficiência e celeridade na resolução do conflito. O estudo tem como objetivo examinar os princípios basilares, os dispositivos legais correspondentes, as características do negócio jurídico processual e, sobretudo, os limites formais e objetivos à liberdade das partes. Dessa forma, revela-se a importância da pesquisa para contribuir com os estudos acerca das particularidades dos negócios processuais, sua incidência, requisitos essenciais e limitações. A indagação do estudo é identificar hipóteses de admissibilidade do negócio jurídico processual. A pesquisa foi pautada nos métodos comparativo e dialético, com a utilização do meio de pesquisa bibliográfico. Com o desenvolvimento do estudo, conclui-se que o negócio jurídico processual tem sido utilizado e admitido na jurisprudência, porém reconhece-se que, para atingir a finalidade, sem desvirtuar o instituto, é crucial delinear o acordo com limites razoáveis a atuação das partes, todavia sem obstar a participação dos sujeitos processuais.
DELAÇÃO PREMIADA: O DESAFIO DA PONDERAÇÃO ENTRE A NECESSIDADE DE SE COMBATER O CRIME ORGANIZADO E A OBSERVÂNCIA DOS DIREITOS E GARANTIAS FUNDAMENTAIS DO ACUSADO
Authors: SILVA Lívia Maria Lopes e , BARBOSA André Luis Jardini
Published: May 2020

O objetivo geral deste trabalho foi analisar a pertinência da Delação Premiada e os pressupostos para sua introdução na legislação pátria; a percepção da mesma pela sociedade; e as consequências do uso contemporâneo do instituto, com foco na questão da (in) compatibilidade da mesma com os direitos e garantias fundamentais dos acusados, verificando até que ponto isto é tolerável. A modernização da sociedade, com a evolução dos meios de comunicação e informação, o fenômeno da globalização, entre outros fatores, acarretou também, inevitavelmente, a sofisticação do crime. Diante desse cenário, a Delação Premiada se mostra como um instrumento eficaz no combate ao crime organizado. No entanto, o procedimento utilizado parece ir de encontro a certas garantias constitucionais dos acusados.
UMA ANÁLISE SOBRE A SELETIVIDADE DA JUSTIÇA CRIMINAL BRASILEIRA
Authors: RITOSSA Elaine Maria Silveira , BARBOSA André Luis Jardini
Published: May 2020

O presente artigo tem como objetivo analisar de maneira sintética de que forma um dos episódios mais sombrios de nossa história, a escravidão, mostra seus reflexos hodiernamente, a partir de uma análise da construção do Estado Democrático de Direito e do advento de teorias punitivistas, como o Direito Penal do Inimigo, ocasionando assim uma latente seletividade racista da justiça criminal brasileira.
O DIREITO DE MORRER DIGNAMENTE: UM ESTUDO SOB A LUZ DO TESTAMENTO VITAL E DO PRINCÍPIO DA AUTONOMIA PRIVADA DA VONTADE
Authors: MORANDO Amanda Ramaiane , CASTRO Fabiana Maria Martins Gomes de
Published: May 2020

O propósito do presente artigo é identificar se há no ordenamento jurídico pátrio o reconhecimento da liberdade e autodeterminação para fazer valer o princípio da dignidade da pessoa humana, tendo por enfoque central o testamento vital. Pois, diante da valorização da dignidade da pessoa humana, permite-se a conclusão de uma vida da forma mais integra e menos dolorosa possível, perto de quem se ama e da forma como se pretende. Utiliza-se, para tanto, o método dedutivo, por meio de um procedimento técnico-bibliográfico sobre o tema, empregando ensinamentos doutrinários e jurisprudenciais, na análise de um acórdão brasileiro, bem como dados secundários, como artigos e teses já publicadas acerca do assunto. É oportuno destacar que, no direito pátrio, ainda não há legislação clara e específica sobre o tema, apoiando-se este, basilarmente, em princípios constitucionais e bioéticos, no Código Civil e nas Resoluções do Conselho Federal de Medicina que traduzem a validade e aplicabilidade da declaração do enfermo sob a forma de testamento vital no contexto jurídico brasileiro. Desta feita, o artigo impinge verificar que o paciente terminal deve ser tratado de modo digno, conforme seus valores, suas crenças ou convicções pessoais, recebendo cuidados paliativos para amenizar o seu sofrer, a fim de assegurar-lhe a qualidade de vida, em virtude de o ser humano possuir outras dimensões que não somente a biológica.
LEI MARIA DA PENHA: ANÁLISE A PARTIR DA VITIMOLOGIA CRÍTICA DE ORIENTAÇÃO FEMINISTA
Authors: FALCAI Maria Fernanda , MARIN Gustavo de Carvalho
Published: May 2020

O presente trabalho foi desenvolvido com o objetivo de analisar a Lei Maria da Penha (LMP), Lei nº 11.340/2006, a partir da vitimologia crítica de orientação feminista. Foram realizados também estudos sobre o movimento feminista, suas peculiaridades, e o impacto que teve para a criação de uma lei que defenda os direitos humanos das mulheres. Neste trabalho foi desenvolvido em ordem cronológica todo o processo até a promulgação da LMP e uma comparação de quando era aplicada aos casos de violência doméstica e familiar, a Lei nº 9.099/95, e o que foi alterado depois que foi retirada sua competência. Conclui-se que A Lei Maria da Penha possui grande representatividade e importância no mundo jurídico, promoveu um alcance maior para a igualdade, suprindo a dívida que o Estado possuía com as mulheres, depois de anos de tratamentos desiguais, em que o homem era visto como detentor de todo o poder, e a mulher como incapaz, submissa a ele. Porém, em relação à prevenção ainda há muito a ser debatido e colocado em prática para a real eficácia da Lei.