bluuebunny/crossref_metadata_2025 huggingface dataset:
Greenness as a Differentiating Strategy
Authors: Masoudi Nahid
Published: June 2021

In a vertical differentiation model, we study a market where consumers, depending on their level of environmental consciousness, value the greenness of the product they consume and are distributed according to a Kumaraswamy distribution. Three scenarios are studied: only one firm takes some green measures and firms compete upon prices; only one firm takes some green measures, and this firm acts as the leader of the price competition; and finally, both firms choose their level of greenness and compete upon their location and price. The results suggest that as consumers become more environmentally conscious, the marginal consumer and the greener firm’s location move to the right. In contrast, the less green firm’s response is non-monotonic. In fact, when the two firms choose their location along with their prices, the latter firm chooses to produce a less green product in response to more environmentally conscious consumers. In the extreme case where all consumers are fully environmentally conscious, the latter firm produces a brown product and sells it at a price equal to its marginal cost. In this case, the greener firm’s price and location choices make the consumers indifferent between the two products. These results could explain why despite all the improvements in the consumers’ environmental consciousness, brown (in its general term) products are still widely produced and consumed, even by environmentally conscious consumers.
Optimal Investment in Preservation Technology for Variable Demand under Trade-Credit and Shortages
Authors: Jani Mrudul Y. , Betheja Manish R. , Chaudhari Urmila , Sarkar Biswajit
Published: June 2021

In particular business transactions, the supplier usually provides an admissible delay in settlement to its vendor to encourage further sales. Additionally, the demand for the commodity is inversely proportional to the function of the sales price, which is non-linear and, in some situations, a holding cost rises over time. Moreover, many goods often deteriorate consistently and shall not be sold after their expiration dates. This study analyses a model for perishable products with a maximum life span with price-dependent demand and trade credit by assimilating these variations and under the supposition of time-varying holding cost. Furthermore, to diminish the rate of deterioration, investment for preservation technology is often taken into account beforehand. Based on real-life circumstances, shortages are admitted and backlogged partially, with an exponential rise in wait time before the new good emerges. The key ambition is to calculate the optimum investment under preservation, sales price, and cycle time using the classical optimization algorithm to maximize the vendor’s net profit. Additionally, to clarify the outcomes, the numerical illustrations are addressed, and the sensitivity analysis of significant parameters is eventually implemented.
An Integrated Decision-Making Approach for Green Supplier Selection in an Agri-Food Supply Chain: Threshold of Robustness Worthiness
Authors: Tirkolaee Erfan Babaee , Dashtian Zahra , Weber Gerhard-Wilhelm , Tomaskova Hana , Soltani Mehdi , Mousavi Nasim Sadat
Published: June 2021

Along with the increased competition in production and service areas, many organizations attempt to provide their products at a lower price and higher quality. On the other hand, consideration of environmental criteria in the conventional supplier selection methodologies is required for companies trying to promote green supply chain management (GSCM). In this regard, a multi-criteria decision-making (MCDM) technique based on analytic hierarchy process (AHP) and fuzzy technique for order of preference by similarity to ideal solution (TOPSIS) is used to evaluate and rate the suppliers. Then, considering the resource constraint, weight of criteria and a rank of suppliers are taken into account in a multi-objective mixed-integer linear programming (MOMILP) to determine the optimum order quantity of each supplier under uncertain conditions. To deal with the uncertain multi-objectiveness of the proposed model, a robust goal programming (RGP) approach based on Shannon entropy is applied. The offered methodology is applied to a real case study from a green service food manufacturing company in Iran in order to verify its applicability with a sensitivity analysis performed on different uncertainty levels. Furthermore, the threshold of robustness worthiness (TRW) is studied by applying different budgets of uncertainty for the green service food manufacturing company. Finally, a discussion and conclusion on the applicability of the methodology is provided, and an outlook to future research projects is given.
How Sustainability Is Defined: An Analysis of 100 Theoretical Approximations
Authors: Luque González Arturo , Coronado Martín Jesús Ángel , Vaca-Tapia Ana Cecilia , Rivas Francklin
Published: June 2021

Sustainability processes are imperfect, hence there is a need to analyze their construction, evolution and deployment. To this end, a sample of one hundred sustainability constructs was taken, together with their conceptual approaches, in order to gauge their impact and to ascertain the dimensions to which they belong. A frequency count and categorization were carried out using Google, which saturated in seven dimensions: economic, social, environmental, legal, political, ethical and cultural. A higher-order association of these hierarchies was then proposed, establishing a triad model that indicated only the most representative combinations of dimensions resulting from the extraction of the most significant definitions. From these definitions and in accordance with their frequency of use in Google, it is inferred that the current concept of sustainability is based on the economic-social-ethical category. This highlights the distance between what, a priori, seems to implicitly allow any definition of sustainability and the existing reality.
Recuperative Amino Acids Separation through Cellulose Derivative Membranes with Microporous Polypropylene Fiber Matrix
Authors: Nechifor Aurelia Cristina , Pîrțac Andreia , Albu Paul Constantin , Grosu Alexandra Raluca , Dumitru Florina , Dimulescu (Nica) Ioana Alina , Oprea Ovidiu , Pașcu Dumitru , Nechifor Gheorghe , Bungău Simona Gabriela
Published: June 2021

The separation, concentration and transport of the amino acids through membranes have been continuously developed due to the multitude of interest amino acids of interest and the sources from which they must be recovered. At the same time, the types of membranes used in the sepa-ration of the amino acids are the most diverse: liquids, ion exchangers, inorganic, polymeric or composites. This paper addresses the recuperative separation of three amino acids (alanine, phe-nylalanine, and methionine) using membranes from cellulosic derivatives in polypropylene ma-trix. The microfiltration membranes (polypropylene hollow fibers) were impregnated with solu-tions of some cellulosic derivatives: cellulose acetate, 2-hydroxyethyl-cellulose, methyl 2-hydroxyethyl-celluloseand sodium carboxymethyl-cellulose. The obtained membranes were characterized in terms of the separation performance of the amino acids considered (retention, flux, and selectivity) and from a morphological and structural point of view: scanning electron microscopy (SEM), high resolution SEM (HR-SEM), Fourier transform infrared spectroscopy (FT-IR), energy dispersive spectroscopy (EDS) and thermal gravimetric analyzer (TGA). The re-sults obtained show that phenylalanine has the highest fluxes through all four types of mem-branes, followed by methionine and alanine. Of the four kinds of membrane, the most suitable for recuperative separation of the considered amino acids are those based on cellulose acetate and methyl 2-hydroxyethyl-cellulose.
A Simple 1D Convection-Diffusion Model of Oxalic Acid Oxidation Using Reactive Electrochemical Membrane
Authors: Skolotneva Ekaterina , Cretin Marc , Mareev Semyon
Published: June 2021

In recent years, electrochemical methods utilizing reactive electrochemical membranes (REM) have been recognized as the most promising technologies for the removal of organic pollutants from water. In this paper, we propose a 1D convection-diffusion-reaction model concerning the transport and oxidation of oxalic acid (OA) and oxygen evolution in the flow-through electrochemical oxidation system with REM. It allows the determination of unknown parameters of the system by treatment of experimental data and predicts the behavior of the electrolysis setup. There is a good agreement in calculated and experimental data at different transmembrane pressures and initial concentrations of OA. The model provides an understanding of the processes occurring in the system and gives the concentration, current density, potential, and overpotential distributions in REM. The dispersion coefficient was determined as a fitting parameter and it is in good agreement with literary data for similar REMs. It is shown that the oxygen evolution reaction plays an important role in the process even under the kinetic limit, and its contribution decreases with increasing total organic carbon flux through the REM.
Hot Deformation Behavior of Ultralight Dual-Phase Mg-6li Alloy: Constitutive Model and Hot Processing Maps
Authors: Li Guo , Bai Xingyu , Peng Qiang , Wei Guobing , Ma Zhenduo
Published: June 2021

High-temperature compression tests with dual-phase Mg-6Li alloy were conducted on the Gleeble-3500 thermal-mechanical simulator. Flow stress and micro-structure evolution were analyzed for temperatures (T = 423, 473,523 and 573 K) and strain rates (ε˙=0.001, 0.01, 0.1 and 1 s−1). On this basis, the constitutive model and hot processing maps were established. Besides, the dynamic re-crystallization (DRX) of α-Mg phase, grain orientation and texture composition under different deformation conditions were analyzed by EBSD technology. The experimental results show that the flow stress of Mg-6Li alloy increased with decreasing deformation temperature and increasing strain rate. In addition, the range of instability zone expanded with the increase of strain. The optimal thermal processing temperature was found to be in the range of 500 K–573 K, and the optimal strain rates were between 0.01 s−1–1 s−1. Model-predicted stress values were compared with experimental values for model verification. The 0.9954 correlation coefficient and the 5.48% average absolute relative error shown by the calculation indicate an acceptable accuracy of the model in predicting thermal deformation behavior of Mg-6Li alloy. Moreover, based on our EBSD data and maps analysis, the DRX proportion of α-Mg phase in Mg-6Li alloy was relatively low, and α-Mg phase formed <0001>//CD basal texture.
Identification of Salivary Microorganisms and Metabolites Associated with Halitosis
Authors: Jo Jae-kwon , Seo Seung-Ho , Park Seong-Eun , Kim Hyun-Woo , Kim Eun-Ju , Na Chang-Su , Cho Kwang-Moon , Kwon Sun-Jae , Moon Young-Ho , Son Hong-Seok
Published: June 2021

Halitosis is mainly caused by the action of oral microbes. The purpose of this study was to investigate the differences in salivary microbes and metabolites between subjects with and without halitosis. Of the 52 participants, 22 were classified into the halitosis group by the volatile sulfur compound analysis on breath samples. The 16S rRNA gene amplicon sequencing and metabolomics approaches were used to investigate the difference in microbes and metabolites in saliva of the control and halitosis groups. The profiles of microbiota and metabolites were relatively different between the halitosis and control groups. The relative abundances of Prevotella, Alloprevotella, and Megasphaera were significantly higher in the halitosis group. In contrast, the relative abundances of Streptococcus, Rothia, and Haemophilus were considerably higher in the control group. The levels of 5-aminovaleric acid and n-acetylornithine were significantly higher in the halitosis group. The correlation between identified metabolites and microbiota reveals that Alloprevotella and Prevotella might be related to the cadaverine and putrescine pathways that cause halitosis. This study could provide insight into the mechanisms of halitosis.
Advancements in Microprocessor Architecture for Ubiquitous AI—An Overview on History, Evolution, and Upcoming Challenges in AI Implementation
Authors: Khan Fatima Hameed , Pasha Muhammad Adeel , Masud Shahid
Published: June 2021

Artificial intelligence (AI) has successfully made its way into contemporary industrial sectors such as automobiles, defense, industrial automation 4.0, healthcare technologies, agriculture, and many other domains because of its ability to act autonomously without continuous human interventions. However, this capability requires processing huge amounts of learning data to extract useful information in real time. The buzz around AI is not new, as this term has been widely known for the past half century. In the 1960s, scientists began to think about machines acting more like humans, which resulted in the development of the first natural language processing computers. It laid the foundation of AI, but there were only a handful of applications until the 1990s due to limitations in processing speed, memory, and computational power available. Since the 1990s, advancements in computer architecture and memory organization have enabled microprocessors to deliver much higher performance. Simultaneously, improvements in the understanding and mathematical representation of AI gave birth to its subset, referred to as machine learning (ML). ML includes different algorithms for independent learning, and the most promising ones are based on brain-inspired techniques classified as artificial neural networks (ANNs). ANNs have subsequently evolved to have deeper and larger structures and are often characterized as deep neural networks (DNN) and convolution neural networks (CNN). In tandem with the emergence of multicore processors, ML techniques started to be embedded in a range of scenarios and applications. Recently, application-specific instruction-set architecture for AI applications has also been supported in different microprocessors. Thus, continuous improvement in microprocessor capabilities has reached a stage where it is now possible to implement complex real-time intelligent applications like computer vision, object identification, speech recognition, data security, spectrum sensing, etc. This paper presents an overview on the evolution of AI and how the increasing capabilities of microprocessors have fueled the adoption of AI in a plethora of application domains. The paper also discusses the upcoming trends in microprocessor architectures and how they will further propel the assimilation of AI in our daily lives.
In Vivo Assessment of Hypoxia Levels in Pancreatic Tumors Using a Dual-Modality Ultrasound/Photoacoustic Imaging System
Authors: Wang Yuhling , Jhang De-Fu , Tsai Chia-Hua , Chiang Nai-Jung , Tsao Chia-Hui , Chuang Chiung-Cheng , Chen Li-Tzong , Chang Wun-Shaing , Liao Lun-De
Published: June 2021

Noninvasive anatomical and functional imaging has become an essential tool to evaluate tissue oxygen saturation dynamics in preclinical or clinical studies of hypoxia. Our dual-wavelength technique for photoacoustic (PA) imaging based on the differential absorbance spectrum of oxyhemoglobin (oxy-Hb) and deoxyhemoglobin (deoxy-Hb) can quantify tissue oxygen saturation using the intrinsic contrast property. PA imaging of tissue oxygen saturation can be used to monitor tumor-related hypoxia, which is a particularly relevant functional parameter of the tumor microenvironment that has a strong influence on tumor aggressiveness. The simultaneous acquisition of anatomical and functional information using dual-modality ultrasound (US) and PA imaging technology enhances the preclinical applicability of the method. Here, the developed dual-modality US/PA system was used to measure relative tissue oxygenation using the dual-wavelength technique. Tissue oxygen saturation was quantified in a pancreatic tumor mouse model. The differences in tissue oxygenation were detected by comparing pancreatic samples from normal and tumor-bearing mice at various time points after implantation. The use of an in vivo pancreatic tumor model revealed changes in hypoxia at various stages of tumor growth. The US/PA imaging data positively correlated with the results of immunohistochemical staining for hypoxia. Thus, our dual-modality US/PA imaging system can be used to reliably assess and monitor hypoxia in pancreatic tumor mouse models. These findings enable the use of a combination of US and PA imaging to acquire anatomical and functional information on tumor growth and to evaluate treatment responses in longitudinal preclinical studies.
Mini-Intein Structures from Extremophiles Suggest a Strategy for Finding Novel Robust Inteins
Authors: Hiltunen Mimmu K. , Beyer Hannes M. , Iwaï Hideo
Published: June 2021

Inteins are prevalent among extremophiles. Mini-inteins with robust splicing properties are of particular interest for biotechnological applications due to their small size. However, biochemical and structural characterization has still been limited to a small number of inteins, and only a few serve as widely used tools in protein engineering. We determined the crystal structure of a naturally occurring Pol-II mini-intein from Pyrococcus horikoshii and compared all three mini-inteins found in the genome of P. horikoshii. Despite their similar sizes, the comparison revealed distinct differences in the insertions and deletions, implying specific evolutionary pathways from distinct ancestral origins. Our studies suggest that sporadically distributed mini-inteins might be more promising for further protein engineering applications than highly conserved mini-inteins. Structural investigations of additional inteins could guide the shortest path to finding novel robust mini-inteins suitable for various protein engineering purposes.
The Fungal Effector Mlp37347 Alters Plasmodesmata Fluxes and Enhances Susceptibility to Pathogen
Authors: Rahman Md. Saifur , Madina Mst Hur , Plourde Mélodie B. , dos Santos Karen Cristine Gonçalves , Huang Xiaoqiang , Zhang Yang , Laliberté Jean-François , Germain Hugo
Published: June 2021

Melampsora larici-populina (Mlp) is a devastating pathogen of poplar trees, causing the defoliating poplar leaf rust disease. Genomic studies have revealed that Mlp possesses a repertoire of 1184 small secreted proteins (SSPs), some of them being characterized as candidate effectors. However, how they promote virulence is still unclear. This study investigates the candidate effector Mlp37347’s role during infection. We developed a stable Arabidopsis transgenic line expressing Mlp37347 tagged with the green fluorescent protein (GFP). We found that the effector accumulated exclusively at plasmodesmata (PD). Moreover, the presence of the effector at plasmodesmata favors enhanced plasmodesmatal flux and reduced callose deposition. Transcriptome profiling and a gene ontology (GO) analysis of transgenic Arabidopsis plants expressing the effector revealed that the genes involved in glucan catabolic processes are up-regulated. This effector has previously been shown to interact with glutamate decarboxylase 1 (GAD1), and in silico docking analysis supported the strong binding between Mlp37347 and GAD1 in this study. In infection assays, the effector promoted Hyalonoperospora arabidopsidis growth but not bacterial growth. Our investigation suggests that the effector Mlp37347 targets PD in host cells and promotes parasitic growth.
Reduced Viscosity of Mg2GeO4 with Minor MgGeO3 between 1000 and 1150 °C Suggests Solid-State Lubrication at the Lithosphere–Asthenosphere Boundary
Authors: Ferrand Thomas , Deldicque Damien
Published: June 2021

Tectonic plates are thought to move above the asthenosphere due to the presence of accumulated melts or volatiles that result in a low-viscosity layer, known as lithosphere–asthenosphere boundary (LAB). Here, we report experiments suggesting that the plates may slide through a solid-state mechanism. Ultrafine-grained aggregates of Mg2GeO4 and minor MgGeO3 were synthetized using spark plasma sintering (SPS) and deformed using a 1-atm deformation rig between 950 °C and 1250 °C. For 1000 < T < 1150 °C, the derivative of the stress–strain relation of the material drops down to zero once a critical stress as low as 30–100 MPa is reached. This viscosity reduction is followed by hardening. The deformation curves are consistent with what is commonly observed in steels during the shear-induced transformation from austenite to martensite, the final material being significantly harder. This is referred to as TRansformation-Induced Plasticity (TRIP), widely observed in metal alloys (TRIP alloys). It should be noted that such enhanced plasticity is not necessarily due to a phase transition, but could consist of any kind of transformation, including structural transformations. We suspect a stress-induced grain-boundary destabilization. This could be associated to the transient existence of a metastable phase forming in the vicinity of grain boundaries between 1000 and 1150 °C. However, no such phase can be observed in the recovered samples. Whatever its nature, the rheological transition seems to occur as a result of a competition between diffusional processes (i.e., thermally activated) and displacive processes (i.e., stress-induced and diffusionless). Consequently, the material would be harder at 1200 °C than at 1100 °C thanks to diffusion that would strengthen thermodynamically stable phases or grain-boundary structures. This alternative scenario for the LAB would not require volatiles. Instead, tectonic plates may slide on a layer in which the peridotite is constantly adjusting via a grain-boundary transformation.
Lead Mobilization and Speciation in Mining Waste: Experiments and Modeling
Authors: Drapeau Clémentine , Argane Rabei , Delolme Cécile , Blanc Denise , Benzaazoua Mostafa , Hakkou Rachid , Baumgartl Thomas , Edraki Mansour , Lassabatere Laurent
Published: June 2021

Mining produces significant amounts of solid mineral waste. Mine waste storage facilities are often challenging to manage and may cause environmental problems. Mining waste is often linked to contaminated mine drainage, including acidic waters with more or less elevated concentrations of trace metals such as lead. This work presents a study on the mobilization of lead from waste from two typical mining sites: Zeida and Mibladen, two now-closed former Pb–Zn mines in the Moulouya region of Morocco. Our research investigates the mobilization potential of Pb from the waste of these mines. The study involved acid–base neutralization capacity tests (ANC–BNC) combined with geochemical modeling. Experimental data allowed for the quantification of the buffering capacity of the samples and the mobilization rates of lead as a function of pH. The geochemical model was fitted to experimental results with thermodynamic considerations. The geochemical model allowed for the identification of the mineral phases involved in providing the buffering capacity of carbonated mining waste (Mibladen) and the meager buffering capacity of the silicate mining waste (Zeida). These cases are representative of contaminated neutral drainage (CND) and acid mine drainage (AMD), respectively. The results highlight the consistency between the ANC–BNC experimental data and the associated modeling in terms of geochemical behavior, validating the approach and identifying the main mechanisms involved. The modeling approach identifies the dissolution of the main solid phases, which impact the pH and the speciation of lead as a function of the pH. This innovative approach, combining ANC–BNC experiments and geochemical modeling, allowed for the accurate identification of mineral phases and surface complexation phenomena, which control the release of lead and its speciation in drainage solutions, as well as within solid phases, as a function of pH.
Temporal–Spatial Separation of Cu from Mo in the Jiama Porphyry Copper–Polymetallic Deposit, Southern Tibet, China
Authors: Zheng Wenbao , Wang Yiyun
Published: June 2021

Jiama is a super-large porphyry copper–polymetallic deposit located in the Gangdese metallogenic belt of southern Tibet. The deposit consists of a combination of a polymetallic skarn, Cu–Mo mineralization at the contact between the Jiama Porphyry and hornfels, and distal Au mineralization in fault. The current metal reserves are 7.4 Mt Cu, 0.6 Mt Mo, 1.8 Mt Pb–Zn, 6.65 Moz Au, and 360.32 Moz Ag, with a skarn to porphyry–hornfels host-rock ratio of ~3:1. Based on detailed field and laboratory investigations, this paper indicates that: (i) the skarn and porphyry–hornfels orebodies are almost entirely preserved; (ii) the emplacement age of the Cu-bearing plutonic rocks is earlier than the plutons containing elevated Mo assays; (iii) the permeability of the wall rocks gradually decreases in an upward direction; (iv) the fluid temperature during the precipitation of Cu was higher than that of the Mo mineralization; (v) the lithospheric pressure during the precipitation of Cu and Mo was the same; (vi) the laser Raman spectroscopy shows that the fluid carrying the Cu was rich in magnetite, hematite, and anhydrite, and the fluid carrying Mo was rich in pyrite, CO2, and CH4; and (vii) the SR–XRF mapping shows that the concentration of Cu in the mineralizing fluid was high and that of Mo was low when Cu was deposited. Conversely, the concentration of Cu was relatively low and the concentration of Mo was relatively high during deposition of the Mo. This study also shows that the temporal and spatial separation of Cu and Mo in the porphyry copper–polymetallic deposit at Jiama was associated with the emplacement of the Jiama Porphyry. Transportation of mineralized fluid was controlled by the permeability of the wall rocks, and deposition of the metals related to changes along a redox front and pressure releases during hydrothermal brecciation at the roof of the Jiama Porphyry.
Quartz Crystallite Size and Moganite Content as Indicators of the Mineralogical Maturity of the Carboniferous Chert: The Case of Cherts from Eastern Asturias (Spain)
Authors: Marcos Celia , de Uribe-Zorita María , Álvarez-Lloret Pedro , Adawy Alaa , Fernández Patricia , Arias Pablo
Published: June 2021

Chert samples from different coastal and inland outcrops in the Eastern Asturias (Spain) were mineralogically investigated for the first time for archaeological purposes. X-ray diffraction, X-ray fluorescence, transmission electron microscopy, infrared and Raman spectroscopy and total organic carbon techniques were used. The low content of moganite, since its detection by X-ray diffraction is practically imperceptible, and the crystallite size (over 1000 Å) of the quartz in these cherts would be indicative of its maturity and could potentially be used for dating chert-tools recovered from archaeological sites. Also, this information can constitute essential data to differentiate the cherts and compare them with those used in archaeological tools. However, neither composition nor crystallite size would allow distinguishing between coastal and inland chert outcrops belonging to the same geological formations.
Using Design of Experiments to Optimize a Screening Analytical Methodology Based on Solid-Phase Microextraction/Gas Chromatography for the Determination of Volatile Methylsiloxanes in Water
Authors: Bernardo Fábio , González-Hernández Providencia , Ratola Nuno , Pino Verónica , Alves Arminda , Homem Vera
Published: June 2021

Volatile methylsiloxanes (VMSs) constitute a group of compounds used in a great variety of products, particularly personal care products. Due to their massive use, they are continually discharged into wastewater treatment plants and are increasingly being detected in wastewater and in the environment at low concentrations. The aim of this work was to develop and validate a fast and reliable methodology to screen seven VMSs in water samples, by headspace solid-phase microextraction (HS-SPME) followed by gas chromatography with flame ionization detection (GC-FID). The influence of several factors affecting the extraction efficiency was investigated using a design of experiments approach. The main factors were selected (fiber type, sample volume, ionic strength, extraction and desorption time, extraction and desorption temperature) and optimized, employing a central composite design. The optimal conditions were: 65 µm PDMS/Divinylbenzene fiber, 10 mL sample, 19.5% NaCl, 39 min extraction time, 10 min desorption time, and 33 °C and 240 °C as extraction and desorption temperature, respectively. The methodology was successfully validated, showing low detection limits (up to 24 ng/L), good precision (relative standard deviations below 15%), and accuracy ranging from 62% to 104% in wastewater, tap, and river water samples.
Activity and Mechanism of Action of Antifungal Peptides from Microorganisms: A Review
Authors: Li Tianxi , Li Lulu , Du Fangyuan , Sun Lei , Shi Jichao , Long Miao , Chen Zeliang
Published: June 2021

Harmful fungi in nature not only cause diseases in plants, but also fungal infection and poisoning when people and animals eat food derived from crops contaminated with them. Unfortunately, such fungi are becoming increasingly more resistant to traditional synthetic antifungal drugs, which can make prevention and control work increasingly more difficult to achieve. This means they are potentially very harmful to human health and lifestyle. Antifungal peptides are natural substances produced by organisms to defend themselves against harmful fungi. As a result, they have become an important research object to help deal with harmful fungi and overcome their drug resistance. Moreover, they are expected to be developed into new therapeutic drugs against drug-resistant fungi in clinical application. This review focuses on antifungal peptides that have been isolated from bacteria, fungi, and other microorganisms to date. Their antifungal activity and factors affecting it are outlined in terms of their antibacterial spectra and effects. The toxic effects of the antifungal peptides and their common solutions are mentioned. The mechanisms of action of the antifungal peptides are described according to their action pathways. The work provides a useful reference for further clinical research and the development of safe antifungal drugs that have high efficiencies and broad application spectra.
Synthesis and Characterizations of PdNi Carbon Supported Nanomaterials: Studies of Electrocatalytic Activity for Oxygen Reduction in Alkaline Medium
Authors: Khan Muhammad , Khattak Rozina , Khan Abbas , Chen Qiuling , Nisar Jan , Iqbal Zahoor , Rashid Abdur , Kamran Abdul , Zekker Ivar , Zahoor Muhammad , Alzahrani Khalid , Batiha Gaber
Published: June 2021

Electrocatalytic materials offer numerous benefits due to their wide range of applications. In this study, a polyol technique was used to synthesize PdNi nanoparticles (NPs) with different percent atomic compositions (Pd = 50 to 90%) to explore their catalytic efficiency. The produced nanoparticles were characterized using X-ray diffraction (XRD) and electrochemical investigations. According to XRD measurements, the synthesized NPs were crystalline in nature, with crystallite sizes of about 2 nm. The electrochemical properties of the synthesized NPs were studied in alkaline solution through a rotating ring-disk electrode (RRDE) technique of cyclic voltammetry. The PdNi nanoparticles supported on carbon (PdNi/C) were used as electrocatalysts and their activity and stability were compared with the homemade Pd/C and Pt/C. In alkaline solution, PdNi/C electrocatalysts showed improved oxygen reduction catalytic activity over benchmark Pd/C and Pt/C electrocatalysts in all composition ratios. Furthermore, stability experiments revealed that PdNi 50:50 is more stable in alkaline solution than pure Pd and other PdNi compositions.
Malaysian Propolis and Metformin Synergistically Mitigate Kidney Oxidative Stress and Inflammation in Streptozotocin-Induced Diabetic Rats
Authors: Nna Victor Udo , Abu Bakar Ainul Bahiyah , Zakaria Zaida , Othman Zaidatul Akmal , Jalil Nur Asyilla Che , Mohamed Mahaneem
Published: June 2021

Diabetic nephropathy is reported to occur as a result of the interactions between several pathophysiological disturbances, as well as renal oxidative stress and inflammation. We examined the effect of Malaysian propolis (MP), which has anti-hyperglycemic, antioxidant and anti-inflammatory properties, on diabetes-induced nephropathy. Diabetic rats were either treated with distilled water (diabetic control (DC) group), MP (300 mg/kg b.w./day), metformin (300 mg/kg b.w./day) or MP + metformin for four weeks. We found significant increases in serum creatinine, urea and uric acid levels, decreases in serum sodium and chloride levels, and increase in kidney lactate dehydrogenase activity in DC group. Furthermore, malondialdehyde level increased significantly, while kidney antioxidant enzymes activities, glutathione level and total antioxidant capacity decreased significantly in DC group. Similarly, kidney immunoexpression of nuclear factor kappa B, tumor necrosis factor-α, interleukin (IL)-1β and caspase-3 increased significantly, while IL-10 immunoexpression decreased significantly in DC group relative to normal control group. Histopathological observations for DC group corroborated the biochemical data. Intervention with MP, metformin or both significantly mitigated these effects and improved renal function, with the best outcome following the combined therapy. MP attenuates diabetic nephropathy and exhibits combined beneficial effect with metformin.
Effects of Pueraria candollei var mirifica (Airy Shaw and Suvat.) Niyomdham on Ovariectomy-Induced Cognitive Impairment and Oxidative Stress in the Mouse Brain
Authors: Chulikhit Yaowared , Sukhano Wichitsak , Daodee Supawadee , Putalun Waraporn , Wongpradit Rakvajee , Khamphukdee Charinya , Umehara Kaoru , Noguchi Hiroshi , Matsumoto Kinzo , Monthakantirat Orawan
Published: June 2021

The effects of the phytoestrogen-enriched plant Pueraria mirifica (PM) extract on ovari-ectomy (OVX)-induced cognitive impairment and hippocampal oxidative stress in mice were investigated. Daily treatment with PM and 17β-estradiol (E2) significantly elevated cognitive behavior as evaluated by using the Y maze test, the novel object recognition test (NORT), and the Morris water maze test (MWM), attenuated atrophic changes in the uterus and decreased serum 17β-estradiol levels. The treatments significantly ameliorated ovariectomy-induced oxidative stress in the hippocampus and serum by a decrease in malondialdehyde (MDA), an enhancement of superoxide dismutase, and catalase activity, including significantly down-regulated expression of IL-1β, IL-6 and TNF-α proinflammatory cytokines, while up-regulating expression of PI3K. The present results suggest that PM extract suppresses oxidative brain damage and dysfunctions in the hippocampal antioxidant system, including the neuroinflammatory system in OVX animals, thereby preventing OVX-induced cognitive impairment. The present results indicate that PM exerts beneficial effects on cognitive deficits for which menopause/ovariectomy have been implicated as risk factors.
Untargeted Phytochemical Profile, Antioxidant Capacity and Enzyme Inhibitory Activity of Cultivated and Wild Lupin Seeds from Tunisia
Authors: Ben Hassine Amna , Rocchetti Gabriele , Zhang Leilei , Senizza Biancamaria , Zengin Gökhan , Mahomoodally Mohamad Fawzi , Ben-Attia Mossadok , Rouphael Youssef , Lucini Luigi , El-Bok Safia
Published: June 2021

Lupin seeds can represent a valuable source of phenolics and other antioxidant compounds. In this work, a comprehensive analysis of the phytochemical profile was performed on seeds from three Lupinus species, including one cultivar (Lupinus albus) and two wild accessions (Lupinus cossentinii and Lupinus luteus), collected from the northern region of Tunisia. Untargeted metabolomic profiling allowed to identify 249 compounds, with a great abundance of phenolics and alkaloids. In this regard, the species L. cossentinii showed the highest phenolic content, being 6.54 mg/g DW, followed by L. luteus (1.60 mg/g DW) and L. albus (1.14 mg/g DW). The in vitro antioxidant capacity measured by the ABTS assay on seed extracts ranged from 4.67 to 17.58 mg trolox equivalents (TE)/g, recording the highest values for L. albus and the lowest for L. luteus. The DPPH radical scavenging activity ranged from 0.39 to 3.50 mg TE/g. FRAP values varied between 4.11 and 5.75 mg TE/g. CUPRAC values for lupin seeds ranged from 7.20 to 8.95 mg TE/g, recording the highest for L. cossentinii. The results of phosphomolybdenum assay and metal chelation showed similarity between the three species of Lupinus. The acetylcholinesterase (AChE) inhibition activity was detected in each methanolic extract analyzed with similar results. Regarding the butyrylcholinesterase (BChE) enzyme, it was weakly inhibited by the Lupinus extracts; in particular, the highest activity values were recorded for L. albus (1.74 mg GALAE/g). Overall, our results showed that L. cossentinii was the most abundant source of polyphenols, consisting mainly in tyrosol equivalents (5.82 mg/g DW). Finally, significant correlations were outlined between the phenolic compounds and the in vitro biological activity measured, particularly when considering flavones, phenolic acids and lower-molecular-weight phenolics.
Epilobium angustifolium L. Extracts as Valuable Ingredients in Cosmetic and Dermatological Products
Authors: Nowak Anna , Zagórska-Dziok Martyna , Ossowicz-Rupniewska Paula , Makuch Edyta , Duchnik Wiktoria , Kucharski Łukasz , Adamiak-Giera Urszula , Prowans Piotr , Czapla Norbert , Bargiel Piotr , Petriczko Jan , Markowska Marta , Klimowicz Adam
Published: June 2021

Epilobium angustifolium L. is a popular and well-known medicinal plant. In this study, an attempt to evaluate the possibility of using this plant in preparations for the care and treatment of skin diseases was made. The antioxidant, antiaging and anti-inflammatory properties of ethanolic extracts from Epilobium angustifolium (FEE) were assessed. Qualitative and quantitative evaluation of extracts chemically composition was performed by gas chromatography with mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC). The total polyphenol content (TPC) of biologically active compounds, such as the total content of polyphenols (TPC), flavonoids (TFC), and assimilation pigments, as well as selected phenolic acids, was assessed. FEE was evaluated for their anti-inflammatory and antiaging properties, achieving 68% inhibition of lipoxygenase activity, 60% of collagenase and 49% of elastase. FEE also showed high antioxidant activity, reaching to 87% of free radical scavenging using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 59% using 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Additionally, in vitro penetration studies were performed using two vehicles, i.e., a hydrogel and an emulsion containing FEE. These studies showed that the active ingredients contained in FEE penetrate through human skin and accumulate in it. The obtained results indicate that E. angustifolium may be an interesting plant material to be applied as a component of cosmetic and dermatological preparations with antiaging and anti-inflammatory properties.
Bisindole Alkaloids from the Alstonia Species: Recent Isolation, Bioactivity, Biosynthesis, and Synthesis
Authors: Pandey Kamal P. , Rahman Md Toufiqur , Cook James M.
Published: June 2021

Bisindoles are structurally complex dimers and are intriguing targets for partial and total synthesis. They exhibit stronger biological activity than their corresponding monomeric units. Alkaloids, including those containing C-19 methyl-substitution in their monomeric units, their synthetic derivatives, and their mismatched pairs can be attractive targets for synthesis and may unlock better drug targets. We herein discuss the isolation of bisindoles from various Alstonia species, their bioactivity, putative biosynthesis, and synthesis. The total synthesis of macralstonidine, macralstonine, O-acetylmacralstonine, and dispegatrine, as well as the partial synthesis of alstonisidine, villalstonine, and macrocarpamine are also discussed in this review. The completion of the total synthesis of pleiocarpamine by Sato et al. completes the formal synthesis of the latter two bisindoles.
Design, Synthesis, Biological Evaluation and Silico Prediction of Novel Sinomenine Derivatives
Authors: Li Shoujie , Gao Mingjie , Nian Xin , Zhang Liyu , Li Jinjie , Cui Dongmei , Zhang Chen , Zhao Changqi
Published: June 2021

Sinomenine is a morphinan alkaloid with a variety of biological activities. Its derivatives have shown significant cytotoxic activity against different cancer cell lines in many studies. In this study, two series of sinomenine derivatives were designed and synthesized by modifying the active positions C1 and C4 on the A ring of sinomenine. Twenty-three compounds were synthesized and characterized by spectroscopy (IR, 1H-NMR, 13C-NMR, and HRMS). They were further evaluated for their cytotoxic activity against five cancer cell lines, MCF-7, Hela, HepG2, SW480 and A549, and a normal cell line, Hek293, using MTT and CCK8 methods. The chlorine-containing compounds exhibited significant cytotoxic activity compared to the nucleus structure of sinomenine. Furthermore, we searched for cancer-related core targets and verified their interaction with derivatives through molecular docking. The chlorine-containing compounds 5g, 5i, 5j, 6a, 6d, 6e, and 6g exhibited the best against four core targets AKT1, EGFR, HARS and KARS. The molecular docking results were consistent with the cytotoxic results. Overall, results indicate that chlorine-containing derivatives might be a promising lead for the development of new anticancer agents.
Paclitaxel-Loaded Magnetic Nanoparticles Based on Biotinylated N-Palmitoyl Chitosan: Synthesis, Characterization and Preliminary In Vitro Studies
Authors: Ursachi Vlad Constantin , Dodi Gianina , Rusu Alina Gabriela , Mihai Cosmin Teodor , Verestiuc Liliana , Balan Vera
Published: June 2021

A considerable interest in cancer research is represented by the development of magnetic nanoparticles based on biofunctionalized polymers for controlled-release systems of hydrophobic chemotherapeutic drugs targeted only to the tumor sites, without affecting normal cells. The objective of the paper is to present the synthesis and in vitro evaluation of the nanocomposites that include a magnetic core able to direct the systems to the target, a polymeric surface shell that provides stabilization and multi-functionality, a chemotherapeutic agent, Paclitaxel (PTX), and a biotin tumor recognition layer. To our best knowledge, there are no studies concerning development of magnetic nanoparticles obtained by partial oxidation, based on biotinylated N-palmitoyl chitosan loaded with PTX. The structure, external morphology, size distribution, colloidal and magnetic properties analyses confirmed the formation of well-defined crystalline magnetite conjugates, with broad distribution, relatively high saturation magnetization and irregular shape. Even if the ability of the nanoparticles to release the drug in 72 h was demonstrated, further complex in vitro and in vivo studies will be performed in order to validate the magnetic nanoparticles as PTX delivery system.
Application of an LC–MS/MS Method for the Simultaneous Quantification of Homovanillic Acid and Vanillylmandelic Acid for the Diagnosis and Follow-Up of Neuroblastoma in 357 Patients
Authors: Hwang Narae , Chong Eunbin , Oh Hyeonju , Cho Hee Won , Lee Ji Won , Sung Ki Woong , Lee Soo-Youn
Published: June 2021

Homovanillic acid (HVA) and vanillylmandelic acid (VMA) are end-stage metabolites of catecholamine and are clinical biomarkers for the diagnosis of neuroblastoma. For the first time in Korea, we implemented and validated a liquid chromatography tandem mass spectrometry (LC–MS/MS) assay to measure urinary concentrations of HVA and VMA according to Clinical and Laboratory Standards Institute guidelines. Our LC–MS/MS assay with minimal sample preparation was validated for linearity, lower limit of detection (LOD), lower limit of quantification (LLOQ), precision, accuracy, extraction recovery, carryover, matrix effect, and method comparison. A total of 1209 measurements was performed to measure HVA and VMA in spot urine between October 2019 and September 2020. The relationship between the two urinary markers, HVA and VMA, was analyzed and exhibited high agreement (89.1% agreement, kappa’s k = 0.6) and a strong correlation (Pearson’s r = 0.73). To our knowledge, this is the first study to utilize LC–MS/MS for simultaneous quantitation of spot urinary HVA and VMA and analyze the clinical application of both markers on a large scale for neuroblastoma patients.
Role of Organic and Eco-Friendly Inhibitors on the Corrosion Mitigation of Steel in Acidic Environments—A State-of-Art Review
Authors: Yang Hyun-Min
Published: June 2021

Steel has versatile application in chemical, structure and construction industries owing to its mechanical properties. However, it is susceptible to corrosion in acid environments. Thus, it requires to protect the steel from corrosion. Different types of corrosion resistance steel, coatings and inhibitors are developed to mitigate the corrosion, but, inhibitor is the best remedies to control the corrosion of steel in acid condition. Moreover, organic and green inhibitors used in acid condition for descaling, acid pickling, pipelines, boiler tubes and oil-wells. Organic inhibitors reduce the dissolution of steel in acid but, it is hazardous, expensive and needs expertise to synthesize the inhibitor. Therefore, there is utmost required to study and compile the latest research about the eco-friendly corrosion inhibitors, which showed more than 90% corrosion inhibition efficiency. In the present study, I have reviewed the state-of-arts, and compile the latest development in organic and eco-friendly corrosion inhibitor used in acid environment as well as suggested about the future scope and role of green inhibitor for sustainable society, which is economical, less hazardous and readily available from the natural sources.
Novel Strategy for the Development of Antibacterial TiO2 Thin Film onto Polymer Substrate at Room Temperature
Authors: Phuinthiang Patcharaporn , Trinh Dang Trung Tri , Channei Duangdao , Ratananikom Khakhanang , Sirilak Sirikasem , Khanitchaidecha Wilawan , Nakaruk Auppatham
Published: June 2021

This work demonstrates a novel method to deposit an antibacterial TiO2 thin film on a polymer substrate at room temperature. A combination of sol–gel and photon assistance was used in the experiment in order to avoid any thermal processes of thin film crystallization. The morphological photograph of samples indicated that the TiO2 thin film was perfectly coated on the PVC substrate without any cracks or pinholes. Chemical analysis by EDS and XPS reported that the thin film consisted of titanium (Ti), oxygen (O), and carbon (C). The Raman spectrum proved that the thin film was the anatase phase of TiO2 and, furthermore, that it was contaminated with carbon remaining from the photon assistance process. In addition, the optical band gap of the thin film was 3.35 eV, suggesting that the photocatalytic activity of TiO2 should occur under UV-A radiation. The bacteria viability assay was examined using E. coli and S. typhimurium as indicator strains under UV-A irradiation (365 nm) at different times. The data from OD and CFU count revealed that >97% of bacteria were killed after 60 min of irradiation, and the bacteria were completely killed at 120 min for E. coli and 180 min for S. typhimurium.