bluuebunny/crossref_metadata_2025 huggingface dataset:
Relationship of the Behavior of Older Participants with Body Composition Change: Results of the Second Wave of the Cognition of Older People, Education, Recreational Activities, Nutrition, Comorbidities, and Functional Capacity Studies (COPERNICUS)
Authors: Kujawska Agnieszka , López Sánchez Guillermo F. , Hoti Flaka , Kujawski Sławomir , Zalewski Paweł , Kędziora-Kornatowska Kornelia
Published: April 2023

Background: To examine the relationship between the frequency of physical activities and food product consumption with body composition change after two years in a sample of older people. Methods: Body composition, mass change, frequency of physical activity, and food products consumption were measured. Depression severity, health self-assessment, cognitive function, and demographic data were included as confounders. Results: There were no significant changes in body composition except for a reduction in visceral fat level within two years (p < 0.05). Drinking beer and eating sweets a few times per week were associated with a significant increase in body fat percentage (p < 0.05). Drinking green or white tea more frequently than a few times per year was related to an increase in body fat (3.18 to 3.88%, p < 0.05). Contrarily, daily consumption of coffee was related to a decrease in body fat (p = 0.029). Subjects who ate sweets once a week or more frequently consumed coffee more often. Conclusions: More frequent drinking of beer or of green or white tea and consumption of sweets were related to an increase in body fat percentage, while daily coffee consumption was related to a decrease in body fat percentage after two years in older, healthy subjects. Noteworthily, the frequencies of food product consumption are interrelated.
Baicalin—Current Trends in Detection Methods and Health-Promoting Properties
Authors: Bajek-Bil Agata , Chmiel Marcelina , Włoch Aleksandra , Stompor-Gorący Monika
Published: April 2023

Baicalin (7-D-glucuronic acid-5,6-dihydroxyflavone) belongs to natural flavonoids extracted from the roots of Scutellaria baicalensis, the plant used in traditional Chinese medicine. It has been proven that baicalin has various pharmacological activities, such as antioxidant, anti-inflammatory, anticancer, antibacterial, and anti-apoptotic ones. However, it is essential not only to determine the medical usefulness of baicalin, but also to find and develop the most effective methods for its extraction and detection. Therefore, the aim of this review was to summarize the current methods of detection and identification of baicalin and to present the medical applications of baicalin and the underlying mechanisms of its action. Based on the review of the latest literature, it can be concluded that liquid chromatography alone or together with mass spectrometry is the most commonly used method for the determination of baicalin. Recently, also new electrochemical methods have been established, e.g., biosensors with fluorescence, which have better detection limits, sensitivity, and selectivity.
Curcumin Epigenetically Represses Histone Acetylation of Echinocandin B Producing Emericella rugulosa
Authors: Kumari Vandana , Kumar Vinay , Kaushal Manisha , Kumar Antresh
Published: April 2023

Echinocandin B is a natural product that possesses potent antifungal property against a wide array of fungi. This antifungal agent is produced by Emericella rugulosa. The biosynthetic genes of echinocandin B are physically organized in two gene clusters (ecd and hty). The ecd gene cluster comprises 12 genes (ecdA–L). On the other hand, hty contains six genes (htyA–F). These gene clusters regulate Echinocandin B expression and play an essential role in chromatin modifications. The present study elucidates the epigenetic regulatory network of echinocandin B production using Histone acetyltransferase (HAT) inhibitor; curcumin using transcriptional gene expression analysis. The High-performance liquid chromatography (HPLC) analysis revealed suppression of the echinocandin B levels in the cells treated with curcumin. Curcumin was also found to repress the expression of different ecd genes by several folds. Taken together, we conclude that curcumin targets echinocandin B production by inhibiting histone acetylation as well as disrupting interspecies consortium communication, which eventually leads to a decrease in the echinocandin B synthesis.
The Mechanism of Resistance of EUROPEAN Plum to Plum pox virus Mediated by Hypersensitive Response Is Linked to VIRAL NIa and Its Protease Activity
Authors: Rodamilans Bernardo , Hadersdorfer Johannes , Berki Zita , García Beatriz , Neumüller Michael , García Juan Antonio
Published: April 2023

Plum pox virus (PPV) infects Prunus trees across the globe, causing the serious Sharka disease. Breeding programs in the past 20 years have been successful, generating plum varieties hypersensitive to PPV that show resistance in the field. Recently, a single tree displaying typical PPV symptoms was detected in an orchard of resistant plums. The tree was eradicated, and infected material was propagated under controlled conditions to study the new PPV isolate. Performing overlapping PCR analysis, the viral sequence was reconstructed, cloned and tested for infectivity in different ‘Jojo’-based resistant plums. The results confirmed that the isolate, named PPV-D ‘Herrenberg’ (PPVD-H), was able to infect all these varieties. Analyses of chimeras between PPVD-H and a PPV-D standard isolate (PPVD) revealed that the NIa region of PPD-H, carrying three amino acid changes, was enough to break the resistance of these plums. Experiments with single and double mutants showed that all changes were essential to preserve the escaping phenotype. Additionally, one of the changes at the VPg-NIapro junction suggested the involvement of controlled endopeptidase cleavage in the viral response. Transient expression experiments in Nicotiana benthamiana confirmed that NIa cleavage in PPVD-H was reduced, compared to PPVD, linking the observed behavior to an NIa cleavage modulation.
Identification of Single-Nucleotide Polymorphisms (SNPs) Associated with Heat Tolerance at the Reproductive Stage in Synthetic Hexaploid Wheats Using GWAS
Authors: Mehvish Ambreen , Aziz Abdul , Bukhari Birra , Qayyum Humaira , Mahmood Zahid , Baber Muhammad , Sajjad Muhammad , Pang Xuequn , Wang Fenglan
Published: April 2023

The projected rise in global ambient temperature by 3–5 °C by the end of this century, along with unpredicted heat waves during critical crop growth stages, can drastically reduce grain yield and will pose a great food security challenge. It is therefore important to identify wheat genetic resources able to withstand high temperatures, discover genes underpinning resilience to higher temperatures, and deploy such genetic resources in wheat breeding to develop heat-tolerant cultivars. In this study, 180 accessions of synthetic hexaploid wheats (SHWs) were evaluated under normal and late wheat growing seasons (to expose them to higher temperatures) at three locations (Islamabad, Bahawalpur, and Tando Jam), and data were collected on 11 morphological and yield-related traits. The diversity panel was genotyped with a 50 K SNP array to conduct genome-wide association studies (GWASs) for heat tolerance in SHW. A known heat-tolerance locus, TaHST1, was profiled to identify different haplotypes of this locus in SHWs and their association with grain yield and related traits in SHWs. There was a 36% decrease in grain yield (GY), a 23% decrease in thousand-grain weight (TKW), and an 18% decrease in grains per spike (GpS) across three locations in the population due to the heat stress conditions. GWASs identified 143 quantitative trait nucleotides (QTNs) distributed over all 21 chromosomes in the SHWs. Out of these, 52 QTNs were associated with morphological and yield-related traits under heat stress, while 15 of them were pleiotropically associated with multiple traits. The heat shock protein (HSP) framework of the wheat genome was then aligned with the QTNs identified in this study. Seventeen QTNs were in proximity to HSPs on chr2B, chr3D, chr5A, chr5B, chr6D, and chr7D. It is likely that QTNs on the D genome and those in proximity to HSPs may carry novel alleles for heat-tolerance genes. The analysis of TaHST1 indicated that 15 haplotypes were present in the SHWs for this locus, while hap1 showed the highest frequency of 25% (33 SHWs). These haplotypes were significantly associated with yield-related traits in the SHWs. New alleles associated with yield-related traits in SHWs could be an excellent reservoir for breeding deployment.
The Effect of Light and Dark Treatment on the Production of Rosmarinic Acid and Biological Activities in Perilla frutescens Microgreens
Authors: Lee Seom , Yeo Hyeon Ji , Lee Sang Yeob , Kim Su Ryang , Park Sang Un , Park Chang Ha
Published: April 2023

This study aimed to investigate the effect of light [a long-day photoperiod (16 h light/8 h dark cycle)] and dark treatment on the production of rosmarinic acid in P. frutescens microgreens and to determine its antioxidant and antibacterial activities. Microgreens of P. frutescens were grown under light and dark conditions and harvested after 10, 15, 20, and 25 days of each treatment. Although dry weight values of microgreens gradually increased from 10 to 25 days of both treatments, the microgreens grown under light treatment possessed slightly higher levels of dry weight than those grown in the dark. Rosmarinic acid and total phenolic content (TPC) were also analyzed using high-performance liquid chromatography (HPLC) and Folin–Ciocalteu assay. The accumulation patterns of rosmarinic acid and TPC gradually increased and decreased, respectively, in P. frutescens microgreens grown in continuous darkness. The highest accumulation was observed in microgreens grown for 20 days. However, rosmarinic acid and TPC values were not significantly different in microgreens grown under light conditions. According to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical inhibition assay, the extracts of P. frutescens microgreens were confirmed to be strong antioxidants, and their ability to scavenge DPPH radicals was positively correlated with the total phenolic content in the microgreens after 10, 15, 20, and 25 days of both treatments. Considering the relatively higher values of dry weight, rosmarinic acid, TPC, and DPPH assay, P. frutescens microgreens after 20 days of darkness and 20 days of light treatment, respectively, were selected for screening antibacterial activity using nine pathogens. Both microgreen extracts showed strong antibacterial activity against pathogens. In particular, the extracts of microgreens grown for 20 days under light treatment showed higher antimicrobial effects. Therefore, the light treatments for 20 days, as well as the darkness treatment for 20 days, were the best conditions for P. frutescens microgreen production because of their high levels of dry weight, phenolics, and biological activities.
Structural Characterization of the Milled-Wood Lignin Isolated from Sweet Orange Tree (Citrus sinensis) Pruning Residue
Authors: Rosado Mario J. , Rencoret Jorge , Gutiérrez Ana , del Río José C.
Published: April 2023

The pruning of sweet orange trees (Citrus sinensis) generates large amounts of lignocellulosic residue. Orange tree pruning (OTP) residue presents a significant lignin content (21.2%). However, there are no previous studies describing the structure of the native lignin in OTPs. In the present work, the “milled-wood lignin” (MWL) was extracted from OTPs and examined in detail via gel permeation chromatography (GPC), pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS), and two-dimensional nuclear magnetic resonance (2D-NMR). The results indicated that the OTP-MWL was mainly composed of guaiacyl (G) units, followed by syringyl (S) units and minor amounts of p-hydroxyphenyl (H) units (H:G:S composition of 1:62:37). The predominance of G-units had a strong influence on the abundance of the different linkages; therefore, although the most abundant linkages were β–O–4′ alkyl–aryl ethers (70% of total lignin linkages), the lignin also contained significant amounts of phenylcoumarans (15%) and resinols (9%), as well as other condensed linkages such as dibenzodioxocins (3%) and spirodienones (3%). The significant content of condensed linkages will make this lignocellulosic residue more recalcitrant to delignification than other hardwoods with lower content of these linkages.
Evaluation of Sourcing Decision for Hydrogen Supply Chain Using an Integrated Multi-Criteria Decision Analysis (MCDA) Tool
Authors: Ransikarbum Kasin , Chanthakhot Wattana , Glimm Tony , Janmontree Jettarat
Published: April 2023

The use of fossil fuels has caused many environmental issues, including greenhouse gas emissions and associated climate change. Several studies have focused on mitigating this problem. One dynamic direction for emerging sources of future renewable energy is the use of hydrogen energy. In this research, we evaluate the sourcing decision for a hydrogen supply chain in the context of a case study in Thailand using group decision making analysis for policy implications. We use an integrative multi-criteria decision analysis (MCDA) tool which includes an analytic hierarchy process (AHP), fuzzy AHP (FAHP), and data envelopment analysis (DEA) to analyze weighted criteria and sourcing alternatives using data collected from a group of selected experts. A list of criteria related to sustainability paradigms and sourcing decisions for possible use of hydrogen energy, including natural gas, coal, biomass, and water, are evaluated. Our results reveal that political acceptance is considered the most important criterion with a global weight of 0.514 in the context of Thailand. Additionally, natural gas is found to be the foreseeable source for hydrogen production in Thailand with a global weight of 0.313. We also note that the analysis is based on specific data inputs and that an alternative with a lower score does not imply that the source is not worth exploring.
Rice Yield Prediction in Different Growth Environments Using Unmanned Aerial Vehicle-Based Hyperspectral Imaging
Authors: Kurihara Junichi , Nagata Toru , Tomiyama Hiroyuki
Published: April 2023

There are certain growth stages and spectral regions that are optimal for obtaining a high accuracy in rice yield prediction by remote sensing. However, there is insufficient knowledge to establish a yield prediction model widely applicable for growth environments with different meteorological factors. In this study, high temporal resolution remote sensing using unmanned aerial vehicle-based hyperspectral imaging was performed to improve the yield prediction accuracy of paddy rice cultivated in different environments. The normalized difference spectral index, an index derived from canopy reflectance at any two spectral bands, was used for a simple linear regression analysis to estimate the optimum stage and spectral region for yield prediction. Although the highest prediction accuracy was obtained from the red-edge and near-infrared regions at the booting stage, the generalization performance for different growth environments was slightly higher at the heading stage than at the booting stage. The coefficient of determination and the root mean squared percentage error for the heading stage were R2 = 0.858 and RMSPE = 7.52%, and they were R2 = 0.853 and RMSPE = 9.22% for the booting stage, respectively. In addition, a correction by solar radiation was ineffective at improving the prediction accuracy. The results demonstrate the possibility of establishing regression models with a high prediction accuracy from a single remote sensing measurement at the heading stage without using meteorological data correction.
Deep Learning-Based Improved WCM Technique for Soil Moisture Retrieval with Satellite Images
Authors: Nijaguna G. S. , Manjunath D. R. , Abouhawwash Mohamed , Askar S. S. , Basha D. Khalandar , Sengupta Jewel
Published: April 2023

The water cycle around the globe is significantly impacted by the moisture in the soil. However, finding a quick and practical model to cope with the enormous amount of data is a difficult issue for remote sensing practitioners. The traditional methods of measuring soil moisture are inefficient at large sizes, which can be replaced by remote sensing techniques for obtaining soil moisture. While determining the soil moisture, the low return frequency of satellites and the lack of images pose a severe challenge to the current remote sensing techniques. Therefore, this paper suggested a novel technique for Soil Moisture Retrieval. In the initial phase, image acquisition is made. Then, VI indexes (NDVI, GLAI, Green NDVI (GNDVI), and WDRVI features) are derived. Further, an improved Water Cloud Model (WCM) is deployed as a vegetation impact rectification scheme. Finally, soil moisture retrieval is determined by the hybrid model combining Deep Max Out Network (DMN) and Bidirectional Gated Recurrent Unit (Bi-GRU) schemes, whose outputs are then passed on to enhanced score level fusion that offers final results. According to the results, the RMSE of the Hybrid Classifier (Bi-GRU and DMN) method was lower (0.9565) than the RMSE of the Hybrid Classifier methods. The ME values of the HC (Bi-GRU and DMN) were also lower (0.728697) than those of the HC methods without the vegetation index, the HC methods without the presence of water clouds, and the HC methods with traditional water clouds. In comparison to HC (Bi-GRU and DMN), the HC method without vegetation index has a lower error of 0.8219 than the HC method with standard water cloud and the HC method without water cloud.
Remote-Sensing Data and Deep-Learning Techniques in Crop Mapping and Yield Prediction: A Systematic Review
Authors: Joshi Abhasha , Pradhan Biswajeet , Gite Shilpa , Chakraborty Subrata
Published: April 2023

Reliable and timely crop-yield prediction and crop mapping are crucial for food security and decision making in the food industry and in agro-environmental management. The global coverage, rich spectral and spatial information and repetitive nature of remote sensing (RS) data have made them effective tools for mapping crop extent and predicting yield before harvesting. Advanced machine-learning methods, particularly deep learning (DL), can accurately represent the complex features essential for crop mapping and yield predictions by accounting for the nonlinear relationships between variables. The DL algorithm has attained remarkable success in different fields of RS and its use in crop monitoring is also increasing. Although a few reviews cover the use of DL techniques in broader RS and agricultural applications, only a small number of references are made to RS-based crop-mapping and yield-prediction studies. A few recently conducted reviews attempted to provide overviews of the applications of DL in crop-yield prediction. However, they did not cover crop mapping and did not consider some of the critical attributes that reveal the essential issues in the field. This study is one of the first in the literature to provide a thorough systematic review of the important scientific works related to state-of-the-art DL techniques and RS in crop mapping and yield estimation. This review systematically identified 90 papers from databases of peer-reviewed scientific publications and comprehensively reviewed the aspects related to the employed platforms, sensors, input features, architectures, frameworks, training data, spatial distributions of study sites, output scales, evaluation metrics and performances. The review suggests that multiple DL-based solutions using different RS data and DL architectures have been developed in recent years, thereby providing reliable solutions for crop mapping and yield prediction. However, challenges related to scarce training data, the development of effective, efficient and generalisable models and the transparency of predictions should be addressed to implement these solutions at scale for diverse locations and crops.
MFTR-Net: A Multi-Level Features Network with Targeted Regularization for Large-Scale Point Cloud Classification
Authors: Liu Ruyu , Zhang Zhiyong , Dai Liting , Zhang Guodao , Sun Bo
Published: April 2023

There are some irregular and disordered noise points in large-scale point clouds, and the accuracy of existing large-scale point cloud classification methods still needs further improvement. This paper proposes a network named MFTR-Net, which considers the local point cloud’s eigenvalue calculation. The eigenvalues of 3D point cloud data and the 2D eigenvalues of projected point clouds on different planes are calculated to express the local feature relationship between adjacent point clouds. A regular point cloud feature image is constructed and inputs into the designed convolutional neural network. The network adds TargetDrop to be more robust. The experimental result shows that our methods can learn more high-dimensional feature information, further improving point cloud classification, and our approach can achieve 98.0% accuracy with the Oakland 3D dataset.
A Complex of Marine Geophysical Methods for Studying Gas Emission Process on the Arctic Shelf
Authors: Krylov Artem A. , Ananiev Roman A. , Chernykh Denis V. , Alekseev Dmitry A. , Balikhin Ermolay I. , Dmitrevsky Nikolay N. , Novikov Mikhail A. , Radiuk Elena A. , Domaniuk Anna V. , Kovachev Sergey A. , Timashkevich Georgy K. , Ivanov Vladimir N. , Ilinsky Dmitry A. , Ganzha Oleg Yu. , Gunar Alexey Yu. , Pushkarev Pavel Yu. , Koshurnikov Andrey V. , Lobkovsky Leopold I. , Semiletov Igor P.
Published: April 2023

The Russian sector of the arctic shelf is the longest in the world. Quite a lot of places of massive discharge of bubble methane from the seabed into the water column and further into the atmosphere were found there. This natural phenomenon requires an extensive complex of geological, biological, geophysical, and chemical studies. This article is devoted to aspects of the use of a complex of marine geophysical equipment applied in the Russian sector of the arctic shelf for the detection and study of areas of the water and sedimentary strata with increased saturation with natural gases, as well as a description of some of the results obtained. This complex contains a single-beam scientific high-frequency echo sounder and multibeam system, a sub-bottom profiler, ocean-bottom seismographs, and equipment for continuous seismoacoustic profiling and electrical exploration. The experience of using the above equipment and the examples of the results obtained in the Laptev Sea have shown that these marine geophysical methods are effective and of particular importance for solving most problems related to the detection, mapping, quantification, and monitoring of underwater gas release from the bottom sediments of the shelf zone of the arctic seas, as well as the study of upper and deeper geological roots of gas emission and their relationship with tectonic processes. Geophysical surveys have a significant performance advantage compared to any contact methods. The large-scale application of a wide range of marine geophysical methods is essential for a comprehensive study of the geohazards of vast shelf zones, which have significant potential for economic use.
COQ7-Related Juvenile-Onset Motor Neuronopathy: A New Pathogenetic Dysfunction Associated with Motor Neuron Disease
Authors: Souza Paulo Victor Sgobbi de , Farias Igor Braga , Serrano Paulo de Lima , Badia Bruno de Mattos Lombardi , Jorge Ana Carolina dos Santos , Barros Glenda Barbosa , Oliveira Hélvia Bertoldo de , Calil Samia Rogatis , Fernandes Isabela Danziato , Ribeiro Roberta Correa , Braga Vinícius Lopes , Machado Roberta Ismael Lacerda , Chieia Marco Antônio Troccoli , Pinto Wladimir Bocca Vieira de Rezende , Oliveira Acary Souza Bulle
Published: April 2023

A 38-year-old Brazilian man presented with slowly progressive quadriparesis since age 11 years. He progressed over 15 years with symptoms restricted to the lower limbs, and since then, with a progressive compromise of the upper limbs. His deceased brother had a similar clinical presentation. Examination showed spastic dysarthria, global amyotrophy, brisk tendon reflexes in the lower limbs, symmetrical quadriparesis, and fasciculations in the four limbs. Neurophysiological studies disclosed acute and chronic signs of denervation and chronic reinnervation involving the cervical, thoracic, and lumbosacral myotomes, with normal sensory conduction study. Fibrillation potentials, fasciculations, and positive sharp waves involved mainly the upper limbs. A diagnosis of long-standing juvenile-onset motor neuronopathy was established. Genetic testing identified the possibly pathogenic variant c.3G>T (p.Met1?) in homozygosity in the COQ7 gene. This report highlights the importance of considering a potentially treatable metabolic dysfunction as the primary mechanism in cases of juvenile motor neuron disease.
Experimental Investigation on Solar Water Heater Integrated with Thermal Battery Using Phase Change Material and Porous Media
Authors: Naghavi Mohammad S. , Silakhori Mahyar , Ang Bee Chin , Simon Cornelis Metselaar Hendrik , Mousavi Gazafroudi Sayed Mohammad , Noorollahi Younes
Published: April 2023

Evacuated tube heat pipe solar collector as a passive solar water heating system is a simple, reliable, and cost-effective way to capture the sun’s thermal energy to supply hot water to homes. In the proposed system, the manifold is reshaped to a tank and filled with phase change materials (PCM) and porous media, which the PCM acts as a latent heat thermal energy storage medium. In order to increase the heat flux from the heat pipe to the PCM and overcome the low thermal conductivity of the PCM, porous media is used. The porous media is connected to the heat pipe condenser to collect the heat and distribute it uniformly throughout the PCM filling the pores. This design of the manifold acts as a heat storage tank or thermal battery. Another pipe in the tank transfers heat from the PCM to the water. Experiments were conducted in 2 modes: charging/discharging and periodic draw-off. The results demonstrated that this thermal battery design could provide homes with the hot water they require on sunny days, while it needs an auxiliary heater or larger solar collector to provide enough hot water on rainy/cloudy days. Considering the solar radiation fluctuation, the efficiency of the thermal battery is 50% ± 9.3%. The thermal battery can warm up the cold water higher than the operating temperature on a sunny day (more than 120 L per day at 38 °C). Using porous media provides better heat distribution in the PCM.
Does Service Trade Liberalization Promote Service Productivity? Evidence from China
Authors: Fu Xin , Wang Tangyou , Yang Hongxu
Published: April 2023

This paper investigates the effects of service trade liberalization on service productivity. Based on a city-level database from 2006 to 2019, we estimate the labor productivity of the service industry and show the varying trends of productivity growth. Additionally, by exploiting the cross-city, cross-time variation at the time of China’s innovation pilot city policy of service trade liberalization, we employ a difference-in-difference strategy to estimate the effects. The empirical evidence suggests that liberalizing the service trade has a positive effect on service productivity. In addition, the evidence indicates that service trade liberalization could increase the productivity of the service industry in cities located in the eastern and central parts of China, as well as in those cities with a higher degree of marketization. The main influencing channels of service trade liberalization on productivity predominantly occur through the increase in technology spillover, market competition, and human capital. China’s evidence highlights the positive relationship between trade liberalization and productivity in the service industry and provides implications for realizing the sustainable development of services in developing countries; therefore, China and other developing countries are suggested to continuously formulate and deepen their service trade liberalization strategy.
Using the PLS-SEM Model to Measure the Impact of the Knowledge Economy on Sustainable Development in the Al-Jouf Region of Saudi Arabia
Authors: Fahad S. Almawishir Naeimah , Benlaria Houcine
Published: April 2023

Saudi Arabia has been increasingly using the knowledge economy, which employs digital technologies, to boost the country’s prosperity and growth. To measure the impact of the knowledge economy on sustainable development in the Al-Jouf region, in this research, a survey was utilized as the principal tool for gathering data from employees who occupy administrative positions in both public and private organizations in the region. The collected data were analyzed using the Partial Least Squares Structural Equation Modeling (PLS-SEM) methodology. The results indicate that information and communication technology (ICT) is the most important dimension in both public and private institutions, while the dimension of innovation, research, and development ranks last. Although ICT is significantly applied, there are shortcomings in other aspects of the knowledge economy in both public and private institutions. This study recommends developing the investment environment through economic incentives and institutional systems to make it more attractive to establish entrepreneurial activities. Additionally, it suggests the need to fund applied research and development and leverage the outcomes to develop innovative and entrepreneurial activities. Overall, the knowledge economy has a positive impact on sustainable development in the Al-Jouf region.
Innovation Activities and Their Impact on Product Innovation Results: Evidence from a Sectorial Study
Authors: Chang-Muñoz Eduardo Antonio , Guarín-García Andrés Felipe , Charris-Sevilla Yuneidis , Gallego-Nicholls José Fernando , Santos-Rojo Cristina , Ortigosa-Blanch Arturo
Published: April 2023

In the current competitive context, innovation has become a necessity, to the point that it seems that no company can survive without developing innovation processes. One of the great concerns of small and medium-sized companies is about the investment of resources in innovation activities and their success. Therefore, this article analyzes the influence of different innovation activities performed by small and medium-sized enterprises (SMEs) of the Colombian Caribbean region on their product innovation outcomes and thereby provides a solid groundwork for the design of innovation strategies. To achieve this objective, a data collection instrument was first validated in 56 SMEs, followed by the application of a reflective measurement model with convergent validity analysis. The results of the model application revealed that investment in Research and Development (R&D) and innovation, knowledge transfer, technology adoption, and financial aspects explain to a greater extent the introduction of new products to the market.
Tetrodotoxin and the Geographic Distribution of the Blue-Lined Octopus Hapalochlaena fasciata on the Korean Coast
Authors: Kim Ji-Hoe , Kim Dong-Wook , Cho Sung-Rae , Lee Ka-Jeong , Mok Jong-Soo
Published: April 2023

The genus Hapalochlaena, including the blue-lined octopus Hapalochlaena fasciata (H. fasciata), is highly toxic. Venomous, blue-lined octopuses were recently found in Korea, but their toxicity, toxin composition, and distribution remain largely unknown. Here we estimated the geographic distribution of the organisms along the Korean coast and clarified their toxicity. Tetrodotoxin (TTX) was present in all three specimens of H. fasciata examined, although the toxicity varied largely between individuals. The mean TTX concentration in the whole body of the three specimens was 6.5 ± 2.2 μg/g (range 3.3–8.5 μg/g). Among the body parts examined, the salivary glands exhibited the highest concentration (22.4 ± 9.7 μg/g). From 2012 to 2021, 26 individuals were obtained nearly every month from different regions of the Korean coast. A non-fatal case of a blue-lined octopus bite was reported along the Korean coast in June 2015. This is the first report on the widespread distribution of blue-lined octopuses on the Korean coast and TTX detection. The widespread distribution of the TTX-bearing H. fasciata along the Korean coast within the temperate zone indicates that the species may soon become a serious health issue in Korea. The toxicity of this species is also a potentially significant human health risk.
Seroepidemiological Survey of Anti-Toxoplasma gondii and Anti-Neospora caninum Antibodies in Domestic Cats (Felis catus) in Rolim de Moura, State of Rondônia, North Brazil
Authors: Silva Ana Luzia Peixoto , Lima Estefany Ferreira , Silva Filho Geraldo Moreira , Ferreira Larissa Claudino , Campos Beatriz de Andrade , Bison Ividy , Brasil Arthur Willian de Lima , Parentoni Roberta Nunes , Feitosa Thais Ferreira , Vilela Vinícius Longo Ribeiro
Published: April 2023

Epidemiological studies on infections by Toxoplasma gondii and Neospora caninum in cats in the North Region of Brazil are scarce. We intended to assess the seroprevalence in cats of anti-T. gondii and anti-N. caninum antibodies, as well as the associated risk factors that may lead them to contract these infections in Rolim de Moura, state of Rondônia, North Brazil. For this, blood serum samples of 100 cats from different regions of the city were evaluated. To assess possible factors associated with infections, epidemiological questionnaires were applied to tutors. The Immunofluorescence Antibody Test (IFAT) was performed for anti-T. gondii (cutoff 1:16) and anti-N. caninum (cutoff 1:50) antibodies. After identifying the positive samples, antibody titration was performed. The results showed the prevalence of 26% (26/100) of anti-T. gondii antibodies, with titration varying between 1:16 to 1:8192. There were no factors associated with the prevalence of anti-T. gondii antibodies in the multivariate analysis in this study. There was no occurrence of seropositive cats for anti-N. caninum. It was concluded that there was a high prevalence of anti-T. gondii antibodies in cats in Rolim de Moura, state of Rondônia, North Brazil. However, the evaluated animals did not present anti-N. caninum antibodies. Therefore, knowing that T. gondii has different transmission forms, we emphasize the importance of spreading more information to the population about cat’s relevance in the T. gondii life cycle and how to avoid the parasite transmission and proliferation.
A Spatial Autocorrelation Method for Taenia solium Risk Mapping: The Case of Lao PDR
Authors: Larkins Andrew , Bruce Mieghan , Ash Amanda
Published: April 2023

Background: The World Health Organization has identified Taenia solium mapping tools as an important development for intensifying control in hyperendemic areas. Taenia solium has also been identified as a priority by the Lao PDR government. There is a limited understanding of the distribution of T. solium due to inherent diagnostic challenges. Method: Global and local autocorrelation statistics were applied to available risk factor data sourced from national censuses to map the risk of Taenia solium in Lao PDR. Results: Approximately 50% of villages could be considered hot spots for one or more risk factors. Different risk factor hot spots co-occurred in 30% of villages. Twenty per cent of villages were classified as hot spots for the proportion of households owning pigs and another risk factor. Northern Lao PDR was the dominant high-risk area. This is consistent with passive reports, limited surveys, and anecdotal reports. One smaller area in southern Lao PDR was also identified as high-risk. This is of particular interest because T. solium has not previously been investigated in this area. Conclusions: The methods applied provide a simple, rapid, and versatile approach that allows endemic countries to begin mapping the risk of T. solium at a sub-national level.
Investing in the Prevention of Communicable Disease Outbreaks: Fiscal Health Modelling—The Tool of Choice for Assessing Public Finance Sustainability
Authors: van der Schans Simon , Schöttler Marcel H. , van der Schans Jurjen , Connolly Mark P. , Postma Maarten J. , Boersma Cornelis
Published: April 2023

National strategies for preparedness for future outbreaks of COVID-19 often include timely preparedness with vaccines. Fiscal health modelling (FHM) has recently been brought forward as an additional analysis by defining the public economic impact from a governmental perspective. As governments are the main decision-makers concerning pandemic preparedness, this study aimed to develop an FHM framework for infectious diseases in the Netherlands. Based on the Dutch COVID-19 outbreak of 2020 and 2021 and publicly available data on tax income and gross domestic product (GDP), the fiscal impact of COVID-19 was assessed using two approaches. Approach I: Prospective modelling of future fiscal impact based on publicly available laboratory-confirmed COVID-19 cases; and Approach II: Retrospective assessment of the extrapolated tax and benefit income and GDP. Approach I estimated the consequences that can be causally linked to the population counts reducing income taxes by EUR 266 million. The total fiscal loss amounted to EUR 164 million over 2 years (excluding pension payments averted). The total losses in terms of tax income (2020 and 2021) and GDP (2020) (Approach II), were estimated at, respectively, EUR 13.58 billion and EUR 96.3 billion. This study analysed different aspects of a communicable disease outbreak and its influence on government public accounts. The choice of the two presented approaches depends on the perspective of the analysis, the time horizon of the analysis and the availability of data.
Feeding Spray-Dried Porcine Plasma to Pigs Reduces African Swine Fever Virus Load in Infected Pigs and Delays Virus Transmission—Study 1
Authors: Blázquez Elena , Pujols Joan , Rodríguez Fernando , Segalés Joaquim , Rosell Rosa , Campbell Joy , Polo Javier
Published: April 2023

The objective of this study was to evaluate the potential benefits of feeding spray-dried porcine plasma (SDPP) to pigs infected with African swine fever virus (ASFV). Two groups of twelve weaned pigs each were fed with CONVENTIONAL or 8% SDPP enriched diets. Two pigs (trojans)/group) were injected intramuscularly with the pandemic ASFV (Georgia 2007/01) and comingled with the rest of the pigs (1:5 trojan:naïve ratio) to simulate a natural route of transmission. Trojans developed ASF and died within the first week after inoculation, but contact pigs did not develop ASF, viremia, or seroconversion. Therefore, three more trojans per group were introduced to optimize the ASFV transmission (1:2 trojan:naïve ratio). Blood, nasal, and rectal swabs were weekly harvested, and at end of the study ASFV-target organs collected. After the second exposure, rectal temperature of conventionally fed contact pigs increased >40.5 °C while fever was delayed in the SDPP contact pigs. Additionally, PCR Ct values in blood, secretions, and tissue samples were significantly lower (p < 0.05) for CONVENTIONAL compared to SDPP contact pigs. Under these study conditions, contact exposed pigs fed SDPP had delayed ASFV transmission and reduced virus load, likely by enhanced specific T-cell priming after the first ASFV-exposure.
Comparison of Biological, Pharmacological Characteristics, Indications, Contraindications, Efficacy, and Adverse Effects of Inactivated Whole-Virus COVID-19 Vaccines Sinopharm, CoronaVac, and Covaxin: An Observational Study
Authors: Meo Sultan Ayoub , ElToukhy Riham A. , Meo Anusha Sultan , Klonoff David C.
Published: April 2023

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an emerging viral zoonotic illness that has developed a distinctive and threatening situation globally. Worldwide, many vaccines were introduced to fight against the COVID-19 pandemic. The present study aims to compare the bio-pharmacological characteristics, indications, contraindications, efficacy, and adverse effects of inactivated whole-virus COVID-19 vaccines, Sinopharm, CoronaVac, and Covaxin. Initially, 262 documents and 6 international organizations were selected. Finally, 41 articles, fact sheets, and international organizations were included. The data were recorded from the World Health Organization (WHO), Food and Drug Administration (FDA) USA, Web of Science, PubMed, EMBASE, and Scopus. The results demonstrated that these three inactivated whole-virus COVID-19 vaccines, Sinopharm, CoronaVac, and Covaxin, received emergency approval from the FDA/WHO, and all three of these vaccines are beneficial for the prevention of the COVID-19 pandemic. The Sinopharm vaccine has been recommended during pregnancy and for people of all age groups, and the CoronaVac and Covaxin vaccines are recommended for people over 18 years of age and older. These three vaccines have recommended intramuscular doses of 0.5 mL each, with a 3–4 week interval. These three vaccines can be stored in a refrigerator at +2 to +8 °C. The common adverse effects of these vaccines are pain at the injection site, redness, fatigue, headache, myalgias, general lethargy, body ache, arthralgia, nausea, chills, fever, and dizziness. The overall mean efficiency for the prevention of the COVID-19 disease is 73.78% for Sinopharm, 70.96% for CoronaVac, and 61.80% for Covaxin. In conclusion, all three inactivated whole-virus COVID-19 vaccines, Sinopharm, CoronaVac, and Covaxin, are beneficial for the prevention of the COVID-19 pandemic. However, evidence suggests that the overall impact of Sinopharm is slightly better than that of CoronaVac and Covaxin.
Laboratory Model Test and Field In Situ Test of Distributed Optical Fiber Monitoring of Seepage in a Karst Depression Reservoir Basin
Authors: Yu Bo , Shen Chunyong , Peng Hao , Guo Fawang
Published: April 2023

Karst depressions are ideal places for building reservoirs and stacking wastes, but karst depressions are mostly located in areas with strong karst development, and there is a problem of karst leakage. The study of karst depression monitoring methods can provide technical support for the construction of reservoirs in karst depressions, and leakage monitoring technology based on distributed temperature sensing (DTS) has the clear advantages of a large measurement range, high precision, continuity, wide distribution, large area, etc. In this paper, a temperature-sensing optical cable is first used to conduct leakage monitoring tests in different soil media, and the temperature change curve in different media is obtained, which verifies the feasibility of the heatable temperature-sensing optical cable to identify leakage in soil media with different moisture contents. Then, for the heatable distributed temperature-sensing optical cable, the variation law of the temperature eigenvalues of the sensing optical cable under different seepage velocities is studied using the layout method wrapped with saturated medium-coarse sand, and the relationship between the seepage rate and the temperature eigenvalues was analyzed. A regression formula for quantitative analysis of leakage velocity was established; finally, the manganese slag silo project in Songtao County in Guizhou Province, China, was used to simulate different leakage conditions on site for testing, and the data were compared and analyzed to obtain the route under different conditions. The temperature distribution law of the seepage section verifies the feasibility of the application of heatable temperature-sensing optical cables in the seepage monitoring of karst depressions.
Comparison between Different Technologies (Zerovalent Iron, Coagulation-Flocculation, Adsorption) for Arsenic Treatment at High Concentrations
Authors: Lan Luis E. , Reina Fernando D. , De Seta Graciela E. , Meichtry Jorge M. , Litter Marta I.
Published: April 2023

The presence of arsenic in water for human consumption is of concern, especially in developing countries, and the design of simple and economic treatments for arsenic removal is imperative. In this paper, three low-cost technologies were evaluated for As(V) or As(III) (5 mg L−1) removal: (1) zerovalent iron (Fe(0)), as powdered (μFe(0)) and iron wool (wFe(0)); (2) coagulation-flocculation with Al2(SO4)3 or FeCl3; and (3) adsorption on a natural clay. μFe(0) was more efficient than wFe(0), requiring a minimal dose of 0.25 g L−1 to achieve [As(V)] < 0.01 mg L−1 after 288 h; the reaction time was reduced to 168 h under stirring. When starting from As(III), partial oxidation to As(V) was observed, and removal was not complete even after 648 h with 1 g L−1 μFe(0). As(V) removal using FeCl3 and Al2(SO4)3 was very fast and completed in 15 min with 0.25 g L−1 of both reagents. However, Al2(SO4)3 was not efficient to remove As(III). With the clay, doses higher than 50 g L−1 and times longer than 648 h were needed to remove both As species. Arsenic leached from μFe(0) used to treat As(III) was almost negligible. Thus, Fe(0) may be the best alternative for low-cost, small-scale applications.
Classroom Psychomotor Education Programme to Enhance Executive Functions: A Cluster Randomised Feasibility Trial
Authors: Riga Vassiliki , Rouvali Aimilia
Published: April 2023

An increase in children exhibiting attention difficulties has created the need for more classroom-based intervention programmes. A promising link between physical education and improvement in executive functioning has been at the centre of attention. POTENTIAL constitutes a novel classroom-based psychomotor education programme to enhance students’ attention and listening skills by improving executive functions. A cluster randomised feasibility study was conducted to explore the feasibility of a definitive trial to assess POTENTIAL’s effectiveness regarding (i) recruitment and sampling procedures; (ii) compliance and fidelity; (iii) the acceptability of POTENTIAL by teachers and children; and (iv) the appropriateness of the outcome measures. Four early years classes with an inclusion unit participated: two implemented POTENTIAL and two received no intervention. Eight children in each class (n = 32) were sampled to investigate the appropriateness of the outcome measures. Teachers’ diaries were utilised to explore the acceptability of the activities and the fidelity and compliance to the implementation. The findings regarding POTENTIAL’s acceptability were positive. The recruitment targets were met, and compliance and fidelity were good. Mixed results were produced about the appropriateness of the outcome measures. Thus, the trial protocol could be scaled up in a definitive trial. This study highlights the need for more physical education programmes to support children’s executive functioning.
Cultural experiences with narratives
Authors:   filmmaker, independent researcher (Armenia), Virapyan Ed.G.
Published: April 2023

Bacchante. Pyro rating. Aesop. O'Henry. Fellini: in search of imperishable meanings. Virgil. Momsen: Germans in the night. Boccaccio: mute. The Taviani brothers: a journey. Process. Keywords: narration, genre, creative method, author, cultural history.