bluuebunny/crossref_metadata_2025 huggingface dataset:
A Bcl11bN797K variant isolated from an immunodeficient patient inhibits early thymocyte development in mice
Authors: Matsumoto Kazuaki , Okuyama Kazuki , Sidwell Tom , Yamashita Motoi , Endo Takaho , Satoh-Takayama Naoko , Ohno Hiroshi , Morio Tomohiro , Rothenberg Ellen V. , Taniuchi Ichiro
Published: March 2024

BCL11B is a transcription factor with six C2H2-type zinc-finger domains. Studies in mice have shown that Bcl11b plays essential roles in T cell development. Several germline heterozygous BCL11B variants have been identified in human patients with inborn errors of immunity (IEI) patients. Among these, two de novo mis-sense variants cause asparagine (N) to lysine (K) replacement in distinct zinc-finger domains, BCL11BN441K and BCL11BN807K. To elucidate the pathogenesis of the BCL11BN807K variant, we generated a mouse model of BCL11BN807K by inserting the corresponding mutation, Bcl11bN797K, into the mouse genome. In Bcl11b+/N797K mice, the proportion of immature CD4−CD8+ single-positive thymocytes was increased, and the development of invariant natural killer cells was severely inhibited in a T-cell-intrinsic manner. Under competitive conditions, γδT cell development was outcompeted by control cells. Bcl11bN797K/N797K mice died within one day of birth. Recipient mice reconstituted with Bcl11bN797K/N797K fetal liver cells nearly lacked CD4+CD8+ double-positive thymocytes, which was consistent with the lack of their emergence in culture from Bcl11bN797K/N797K fetal liver progenitors. Interestingly, Bcl11bN797K/N797K progenitors gave rise to aberrant c-Kit+ and CD44+ cells both in vivo and in vitro. The increase in the proportion of immature CD8 single-positive thymocytes in the Bcl11bN797K mutants is caused, in part, by the inefficient activation of the Cd4 gene due to the attenuated function of the two Cd4 enhancers via distinct mechanisms. Therefore, we conclude that immunodeficient patient-derived Bcl11bN797K mutant mice elucidated a novel role for Bcl11b in driving the appropriate transition of CD4−CD8− into CD4+CD8+ thymocytes.
Geospatial analysis of shoreline changes in the Oman coastal region (2000-2022) using GIS and remote sensing techniques
Authors: Colak Arife Tugsan Isiacik
Published: March 2024

This research introduces an innovative method employing the Canny edge detector for automatic and precise coastline extraction, aiming to analyze spatial and temporal variations in the Oman coastline from 2000 to 2022 using GIS and remote sensing (RS) techniques. Focusing on both multi-decadal and short-term periods, the study aims to detect accretion and erosion rates through the observation and interpretation of coastal changes. Utilizing the Digital Shoreline Analysis System and LANDSAT imageries, Shoreline changes have been quantitatively evaluated using three distinct approaches: Linear Regression Rate (LRR), End Point Rate (EPR), and Net Shoreline Movement (NSM). The dynamic nature of the Oman coastal region necessitates a comprehensive understanding of its evolving coastline. Our investigation applies digital shoreline analysis to discern shifts in the coastline’s position, employing a multiple regression approach for quantifying the rate of coastal change. To facilitate automatic shoreline extraction, various methods were experimented with, ultimately determining the Canny Edge algorithm’s superiority in yielding precise results. The paper outlines the monitoring procedures for the coastal area and analyzes coastline changes using geospatial techniques. This analysis provides valuable insights for the planning and management of the Oman shore. Furthermore, the proposed model’s applicability is rigorously tested against other generic edge detection algorithms, including Sobel, Prewitt, and Robert’s techniques. The conclusive findings demonstrate that our model outperforms these alternatives, particularly excelling in the accurate detection of the coastline. This research contributes to a deeper understanding of coastal dynamics and offers a robust methodology for coastal monitoring, with implications for effective planning and management strategies in the Oman shore region.
An optimized culturomics strategy for isolation of human milk microbiota
Authors: Wang Fan , Yu Lingmin , Ren Yuting , Zhang Qianwen , He Shanshan , Zhao Minlei , He Zhili , Gao Qi , Chen Jianguo
Published: March 2024

Viable microorganisms and a diverse microbial ecosystem found in human milk play a crucial role in promoting healthy immune system and shaping the microbial community in the infant’s gut. Culturomics is a method to obtain a comprehensive repertoire of human milk microbiota. However, culturomics is an onerous procedure, and needs expertise, making it difficult to be widely implemented. Currently, there is no efficient and feasible culturomics method specifically designed for human milk microbiota yet. Therefore, the aim of this study was to develop a more efficient and feasible culturomics method specifically designed for human milk microbiota. We obtained fresh samples of human milk from healthy Chinese mothers and conducted a 27-day enrichment process using blood culture bottles. Bacterial isolates were harvested at different time intervals and cultured on four different types of media. Using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, we identified a total of 6601 colonies and successfully obtained 865 strains, representing 4 phyla, 21 genera, and 54 species. By combining CBA and MRS media, we were able to cultivate over 94.4% of bacterial species with high diversity, including species-specific microorganisms. Prolonged pre-incubation in blood culture bottles significantly increased the number of bacterial species by about 33% and improved the isolation efficiency of beneficial bacteria with low abundance in human milk. After optimization, we reduced the pre-incubation time in blood culture bottles and selected optimal picking time-points (0, 3, and 6 days) at 37°C. By testing 6601 colonies using MALDI-TOF MS, we estimated that this new protocol could obtain more than 90% of bacterial species, reducing the workload by 57.0%. In conclusion, our new culturomics strategy, which involves the combination of CBA and MRS media, extended pre-incubation enrichment, and optimized picking time-points, is a feasible method for studying the human milk microbiota. This protocol significantly improves the efficiency of culturomics and allows for the establishment of a comprehensive repertoire of bacterial species and strains in human milk.
Sex differences in the context dependency of episodic memory
Authors: Le Aliza A. , Palmer Linda C. , Chavez Jasmine , Gall Christine M. , Lynch Gary
Published: March 2024

Context contributes to multiple aspects of human episodic memory including segmentation and retrieval. The present studies tested if, in adult male and female mice, context influences the encoding of odors encountered in a single unsupervised sampling session of the type used for the routine acquisition of episodic memories. The three paradigms used differed in complexity (single vs. multiple odor cues) and period from sampling to testing. Results show that males consistently encode odors in a context-dependent manner: the mice discriminated novel from previously sampled cues when tested in the chamber of initial cue sampling but not in a distinct yet familiar chamber. This was independent of the interval between cue encounters or the latency from initial sampling to testing. In contrast, female mice acquired both single cues and the elements of multi-cue episodes, but recall of that information was dependent upon the surrounding context only when the cues were presented serially. These results extend the list of episodic memory features expressed by rodents and also introduce a striking and unexpected sex difference in context effects.
Associations between genetically determined dietary factors and risk of autism spectrum disorder: a Mendelian randomization study
Authors: Li Wenwen , Liu Cuncheng , Chen Shouqiang
Published: March 2024

Background

Existing studies confirm the importance of dietary factors in developing autism spectrum disorder (ASD) and disease progression. Still, these studies are primarily observational, and their causal relationship is unknown. Moreover, due to the extensive diversity of food types, the existing research remains somewhat limited in comprehensiveness. The inconsistency of the results of some studies is very disruptive to the clinic. This study infers a causal relationship between dietary factors on the risk of developing ASD from a genetic perspective, which may lead to significant low-cost benefits for children with ASD once the specificity of dietary factors interfering with ASD is confirmed.

Methods

We performed a two-sample Mendelian randomization (MR) analysis by selecting single nucleotide polymorphisms (SNPs) for 18 common dietary factors from the genome-wide association study (GWAS) database as instrumental variables (IVs) and obtaining pooled data for ASD (Sample size = 46,351) from the iPSYCH-PGC institution. Inverse variance weighted (IVW) was used as the primary analytical method to estimate causality, Cochran's Q test to assess heterogeneity, the Egger-intercept test to test for pleiotropy and sensitivity analysis to verify the reliability of causal association results.

Results

The MR analysis identified four dietary factors with potential causal relationships: poultry intake (fixed-effects IVW: OR = 0.245, 95% CI: 0.084–0.718, P < 0.05), beef intake (fixed-effects IVW: OR = 0.380, 95% CI: 0.165–0.874, P < 0.05), cheese intake (random-effects IVW: OR = 1.526, 95% CI: 1.003–2.321, P < 0.05), and dried fruit intake (fixed-effects IVW: OR = 2.167, 95% CI: 1.342–3.501, P < 0.05). There was no causal relationship between the remaining 14 dietary factors and ASD (P > 0.05).

Conclusion

This study revealed potential causal relationships between poultry intake, beef intake, cheese intake, dried fruit intake, and ASD. Poultry and beef intake were associated with a reduced risk of ASD, while cheese and dried fruit intake were associated with an increased risk. Other dietary factors included in this study were not associated with ASD.
The effect of micronutrient on thyroid cancer risk: a Mendelian randomization study
Authors: Shen Jiali , Zhang Hong , Jiang Hongzhan , Lin Huihui , He Jiaxi , Fan Siyue , Yu Doudou , Yang Liping , Tang Hui , Lin Ende , Li Lianghui , Chen Lijuan
Published: March 2024

Background

The effect of micronutrients on thyroid cancer has been studied in observational studies, however, the cause of relationships has not yet been determined. Thyroid cancer was the subject of a Mendelian randomization (MR) analysis of micronutrients. Aimed to determine whether micronutrient intake has a causal impact on the chance of developing thyroid cancer.

Methods

We used a Mendelian randomization (MR) analysis with two samples. Our circulation levels of Cu, Ir, Zn, Ca, VD, and VC were reflected by genetic variations reported from GWAS in individuals of European ancestry. For the GWAS outcome of thyroid cancer. Sensitivity studies that included MR-Egger, weighted median/mode tests, and a more open selection of variations at a genome-wide sub-significant threshold were added to our inverse-variance weighted (IVW) MR study.

Results

Using the IVW approach, we did not find evidence that any of the micronutrients to thyroid cancer (Cu: odds ratio [OR = 0.88, p = 0.41]; Zn: odds ratio [OR = 0.87, p = 0.40]; Ir: odds ratio [OR = 1.18, p = 0.39]; Ca: odds ratio [OR = 1.12, p = 0.43]; VC: odds ratio [OR = 0.95, p = 0.22]; VD: odds ratio [OR = 0.89, p = 0.04]). The heterogeneity (p > 0.05) and pleiotropy (p > 0.05) testing provided confirmatory evidence for the validity of our MR estimates.

Conclusion

This study does not provide evidence that supplementation with micronutrients including Cu, Ir, Zn, Ca, VD, and VC can prevent thyroid cancer.
Intestinal organoid modeling: bridging the gap from experimental model to clinical translation
Authors: Liu Taotao , Li Xiaoqi , Li Hao , Qin Jingjing , Xu Hui , Wen Jun , He Yaqin , Zhang Cao
Published: March 2024

The 3D culture of intestinal organoids entails embedding isolated intestinal crypts and bone marrow mesenchymal stem cells within a growth factor-enriched matrix gel. This process leads to the formation of hollow microspheres with structures resembling intestinal epithelial cells, which are referred to as intestinal organoids. These structures encompass various functional epithelial cell types found in the small intestine and closely mimic the organizational patterns of the small intestine, earning them the name “mini-intestines”. Intestinal tumors are prevalent within the digestive system and represent a significant menace to human health. Through the application of 3D culture technology, miniature colorectal organs can be cultivated to retain the genetic characteristics of the primary tumor. This innovation offers novel prospects for individualized treatments among patients with intestinal tumors. Presently established libraries of patient-derived organoids serve as potent tools for conducting comprehensive investigations into tissue functionality, developmental processes, tumorigenesis, and the pathobiology of cancer. This review explores the origins of intestinal organoids, their culturing environments, and their advancements in the realm of precision medicine. It also addresses the current challenges and outlines future prospects for development.
Research progress on arsenic, arsenic-containing medicinal materials, and arsenic-containing preparations: clinical application, pharmacological effects, and toxicity
Authors: Yang Yichu , Li Yiye , Li Ran , Wang Zhang
Published: March 2024

Introduction: The toxicity of arsenic is widely recognized globally, mainly harming human health by polluting water, soil, and food. However, its formulations can also be used for the clinical treatment of diseases such as leukemia and tumors. Arsenic has been used as a drug in China for over 2,400 years, with examples such as the arsenic-containing drug realgar mentioned in Shennong’s Herbal Classic. We have reviewed references on arsenic over the past thirty years and found that research has mainly focused on clinical, pharmacological, and toxicological aspects.

Results and Discussion: The finding showed that in clinical practice, arsenic trioxide is mainly used in combination with all-trans retinoic acid (ATRA) at a dose of 10 mg/d for the treatment of acute promyelocytic leukemia (APL); realgar can be used to treat acute promyelocytic leukemia, myelodysplastic syndrome, and lymphoma. In terms of pharmacology, arsenic mainly exerts anti-tumor effects. The dosage range of the action is 0.01–80 μmol/L, and the concentration of arsenic in most studies does not exceed 20 μmol/L. The pharmacological effects of realgar include antiviral activity, inhibition of overactivated lactate dehydrogenase, and resistance to malaria parasites. In terms of toxicity, arsenic is toxic to multiple systems in a dose-dependent manner. For example, 5 μmol/L sodium arsenite can induce liver oxidative damage and promote the expression of pro-inflammatory factors, and 15 μmol/L sodium arsenite induces myocardial injury; when the concentration is higher, it is more likely to cause toxic damage.
‘Seeing’ the electromagnetic spectrum: spotlight on the cryptochrome photocycle
Authors: Aguida Blanche , Babo Jonathan , Baouz Soria , Jourdan Nathalie , Procopio Maria , El-Esawi Mohamed A. , Engle Dorothy , Mills Stephen , Wenkel Stephan , Huck Alexander , Berg-Sørensen Kirstine , Kampranis Sotirios C. , Link Justin , Ahmad Margaret
Published: March 2024

Cryptochromes are widely dispersed flavoprotein photoreceptors that regulate numerous developmental responses to light in plants, as well as to stress and entrainment of the circadian clock in animals and humans. All cryptochromes are closely related to an ancient family of light-absorbing flavoenzymes known as photolyases, which use light as an energy source for DNA repair but themselves have no light sensing role. Here we review the means by which plant cryptochromes acquired a light sensing function. This transition involved subtle changes within the flavin binding pocket which gave rise to a visual photocycle consisting of light-inducible and dark-reversible flavin redox state transitions. In this photocycle, light first triggers flavin reduction from an initial dark-adapted resting state (FADox). The reduced state is the biologically active or ‘lit’ state, correlating with biological activity. Subsequently, the photoreduced flavin reoxidises back to the dark adapted or ‘resting’ state. Because the rate of reoxidation determines the lifetime of the signaling state, it significantly modulates biological activity. As a consequence of this redox photocycle Crys respond to both the wavelength and the intensity of light, but are in addition regulated by factors such as temperature, oxygen concentration, and cellular metabolites that alter rates of flavin reoxidation even independently of light. Mechanistically, flavin reduction is correlated with conformational change in the protein, which is thought to mediate biological activity through interaction with biological signaling partners. In addition, a second, entirely independent signaling mechanism arises from the cryptochrome photocycle in the form of reactive oxygen species (ROS). These are synthesized during flavin reoxidation, are known mediators of biotic and abiotic stress responses, and have been linked to Cry biological activity in plants and animals. Additional special properties arising from the cryptochrome photocycle include responsivity to electromagnetic fields and their applications in optogenetics. Finally, innovations in methodology such as the use of Nitrogen Vacancy (NV) diamond centers to follow cryptochrome magnetic field sensitivity in vivo are discussed, as well as the potential for a whole new technology of ‘magneto-genetics’ for future applications in synthetic biology and medicine.
Differential change in alcohol consumption during the COVID-19 pandemic: the role of loneliness, socialization, and mental well-being
Authors: Mohamed Mohamed S. , Rukh Gull , Vadlin Sofia , Olofsdotter Susanne , Åslund Cecilia , Schiöth Helgi B. , Nilsson Kent W.
Published: March 2024

Introduction

The COVID-19 pandemic led to a surge in mental health issues and psychological distress, disruption to work/studying conditions, and social isolation particularly among young adults. Changes in these factors are differentially associated with alcohol use. Moreover, the relationship between these factors are bidirectional and may have fluctuated throughout the different phases of the pandemic. However, studies focusing on young adults had conflicting results, short follow-up periods, and lacked comprehensive data to describe underlying mechanisms.

Methods

1067 young adults participated in repetitive measures termed wave 4 (2021) of the Survey of Adolescent Life in Västmanland Cohort “SALVe” Cohort. Of these, 889 also completed pre-pandemic measurements termed wave 3 (2018). Participants completed the Alcohol Use Disorders Identification Test (AUDIT) to evaluate alcohol consumption and harmful use. Cross-sectional associations between perceived changes in alcohol use and shift in individual, mental health, and work environment factors were examined using Chi-square tests. Logistic regression was utilized to identify pre-pandemic predictors of harmful consumption during the pandemic.

Results

Harmful consumption decreased only in females following the COVID-19 pandemic. Participants who reported increased feelings of depression, anxiety, and loneliness were more likely to increase their alcohol use. Interestingly, the subgroup who felt less lonely and met their friends more often, as well as those who continued working/studying from their regular workplace also had an increased likelihood of higher consumption. Only pre-pandemic ADHD and delinquency symptoms predicted harmful alcohol consumption following the pandemic.

Conclusion

Females reduced harmful alcohol consumption during the COVID-19 pandemic. While those who suffered the burden of social isolation and distress were more likely to increase their alcohol use, young adults who felt less lonely and met their friends more often also had a similar outcome. The relationship between loneliness and alcohol consumption among young adults is influenced by the social factors that may be facilitated by drinking.
Exposure to urban green spaces and mental health during the COVID-19 pandemic: evidence from two low and lower-middle-income countries
Authors: Patwary Muhammad Mainuddin , Bardhan Mondira , İnan Hüseyin Ertan , Browning Matthew H. E. M. , Disha Asma Safia , Haque Md. Zahidul , Helmy Mai , Ashraf Sadia , Dzhambov Angel M. , Shuvo Faysal Kabir , Alam Md. Ashraful , Billah Sharif Mutasim , Kabir Md. Pervez , Hossain Md. Riad , Azam Md. Golam , Rahman Md. Mijanur , Swed Sarya , Sah Ranjit , Montenegro-Idrogo Juan J. , Bonilla-Aldana D. Katterine , Rodriguez-Morales Alfonso J.
Published: March 2024

Introduction

The COVID-19 pandemic has had a significant impact on mental health globally, with limited access to mental health care affecting low- and middle-income countries (LMICs) the most. In response, alternative strategies to support mental health have been necessary, with access to green spaces being a potential solution. While studies have highlighted the role of green spaces in promoting mental health during pandemic lockdowns, few studies have focused on the role of green spaces in mental health recovery after lockdowns. This study investigated changes in green space access and associations with mental health recovery in Bangladesh and Egypt across the pandemic.

Methods

An online survey was conducted between January and April 2021 after the first lockdown was lifted in Bangladesh (n = 556) and Egypt (n = 660). We evaluated indoor and outdoor greenery, including the number of household plants, window views, and duration of outdoor visits. The quantity of greenness was estimated using the normalized difference vegetation index (NDVI). This index was estimated using satellite images with a resolution of 10x10m during the survey period (January-April 2021) with Sentinel-2 satellite in the Google Earth Engine platform. We calculated averages within 250m, 300m, 500m and 1000m buffers of the survey check-in locations using ArcGIS 10.3. Multiple linear regression models were used to evaluate relationships between changes in natural exposure and changes in mental health.

Results

The results showed that mental health improved in both countries after the lockdown period. People in both countries increased their time spent outdoors in green spaces after the lockdown period, and these increases in time outdoors were associated with improved mental health. Unexpectedly, changes in the number of indoor plants after the lockdown period were associated with contrasting mental health outcomes; more plants translated to increased anxiety and decreased depression. Refocusing lives after the pandemic on areas other than maintaining indoor plants may assist with worrying and feeling panicked. Still, indoor plants may assist with depressive symptoms for people remaining isolated.

Conclusion

These findings have important implications for policymakers and urban planners in LMICs, highlighting the need to increase access to natural environments in urban areas to improve mental health and well-being in public health emergencies.
How are APOE4, changes in body weight, and longevity related? Insights from a causal mediation analysis
Authors: Holmes Rachel , Duan Hongzhe , Bagley Olivia , Wu Deqing , Loika Yury , Kulminski Alexander , Yashin Anatoliy , Arbeev Konstantin , Ukraintseva Svetlana
Published: March 2024

The ε4 allele of the APOE gene (APOE4) is known for its negative association with human longevity; however, the mechanism is unclear. APOE4 is also linked to changes in body weight, and the latter changes were associated with survival in some studies. Here, we explore the role of aging changes in weight in the connection between APOE4 and longevity using the causal mediation analysis (CMA) approach to uncover the mechanisms of genetic associations. Using the Health and Retirement Study (HRS) data, we tested a hypothesis of whether the association of APOE4 with reduced survival to age 85+ is mediated by key characteristics of age trajectories of weight, such as the age at reaching peak values and the slope of the decline in weight afterward. Mediation effects were evaluated by the total effect (TE), natural indirect effect, and percentage mediated. The controlled direct effect and natural direct effect are also reported. The CMA results suggest that APOE4 carriers have 19%–22% (TE p = 0.020–0.039) lower chances of surviving to age 85 and beyond, in part, because they reach peak values of weight at younger ages, and their weight declines faster afterward compared to non-carriers. This finding is in line with the idea that the detrimental effect of APOE4 on longevity is, in part, related to the accelerated physical aging of ε4 carriers.
Exploring the intricate cross-talk between clonal expansion and the bone marrow niche
Authors: Ngo Steven , Papazoglou Despoina , Huerga Encabo Hector , Bonnet Dominique
Published: March 2024

Haematopoietic stem cells (HSCs) reside within an intricate network of cells in the bone marrow (BM) niche. HSC crosstalk with niche compartments influences lineage determination and blood cell production, while independent niche interactions are essential for the maintenance of HSC quiescence. How different niche components influence the genetic diversity of HSCs represents an expanding field of investigation. As such, we will summarise the current knowledge of the contribution to the Darwinian evolution of mutant HSCs of both haematopoietic and non-haematopoietic cells residing in the BM. In this review, we will disentangle how somatic evolution associates with the niche at two stages: from (1) the stage of preleukaemic HSC expansion and clonal haematopoiesis (CH) to (2) leukaemia-initiating cells (LICs) and the development of myeloid malignancies with acute myeloid leukaemia (AML) being the most prevalent. We will finally describe current challenges such as limitations in models used in the field or the difficulty in studying specific genetic clones in isolation.
Social problem-solving workation through collaboration between local regions and urban companies: the case of Kamaishi in Japan
Authors: Matsushita Keita
Published: March 2024

This study analyzed a social problem-solving workation in Kamaishi, Japan, focusing on the “hitohada nugu” experience, a cross-boundary and other-oriented contribution and relationship-building experience. The results of interviews with stakeholders of the work experience, including company managers and participants, intermediaries in the host local community, and government officials, as well as a questionnaire survey of all participants, indicated that employees who participated in the program learned and grew through their inexperience in Kamaishi, which was different from their work experience. On the other hand, the local community and companies that plan and operate the program face a dilemma in explaining the effects of the workation on the company's business, the solution to local issues, and the learning and growth of employees.
Driving frugal innovation in SMEs: how sustainable leadership, knowledge sources and information credibility make a difference
Authors: Ur Rehman Khalil , Anwar Rana Salman , Antohi Valentin Marian , Ali Uzma , Fortea Costinela , Laura Zlati Monica
Published: March 2024

This study investigates the driving factors behind frugal innovation in Small and Medium-sized Enterprises (SMEs). It specifically examines sustainable leadership as an independent variable, considering its impact on frugal innovation, with sources of knowledge mediating this relationship and information credibility moderating the effects. Employing a Partial Least Squares Structural Equation Modeling (PLS-SEM) approach, data were gathered from 325 employees of SMEs in Pakistan. This methodology was chosen for its ability to handle complex relationships between multiple variables simultaneously, offering robust insights into the interplay among sustainable leadership, sources of knowledge, information credibility, and frugal innovation. The results reveal significant associations between sustainable leadership, sources of knowledge, information credibility, and frugal innovation. Sustainable leadership demonstrates a substantial influence on both sources of knowledge and frugal innovation. Furthermore, sources of knowledge play a vital role in mediating the relationship between sustainable leadership and frugal innovation. Information credibility emerges as a significant moderator, affecting the pathways between sustainable leadership, sources of knowledge, and frugal innovation. The findings underscore the importance of sustainable leadership and credible information sources in driving frugal innovation within SMEs. They highlight the intricate interdependencies among these variables and emphasize the pivotal role of information credibility in shaping these dynamics. These results carry significant implications for SMEs in Pakistan, shedding light on the mechanisms through which sustainable leadership and reliable knowledge sources can stimulate frugal innovation in emerging economies.
Development of a rapid quantitative method to differentiate MS1 vaccine strain from wild-type Mycoplasma synoviae
Authors: Liao Changtao , Chen Yiquan , Yan Zhuanqiang , Song Yiwei , Zhou Qi , Zhu Puduo , He Xudong , Li Wenyang , Chen Feng
Published: March 2024

Mycoplasma synoviae (MS) is an economically important pathogen in the poultry industry. Vaccination is an effective method to prevent and control MS infections. Currently two live attenuated MS vaccines are commercially available, the temperature-sensitive MS-H vaccine strain and the NAD-independent MS1 vaccine strain. Differentiation of vaccine strains from wild-type (WT) strains is crucial for monitoring MS infection, especially after vaccination. In this study, we developed a Taqman duplex real-time polymerase chain reaction (PCR) method to identify MS1 vaccine strains from WT strains. The method was specific and did not cross-react with other avian pathogens. The sensitivity assay indicated that no inhibition occurred between probes or between mixed and pure templates in duplex real-time PCR. Compared with the melt-based mismatch amplification mutation assay (MAMA), our method was more sensitive and rapid. In conclusion, the Taqman duplex real-time PCR method is a useful method for the diagnosis and differentiation of WT-MS and MS1 vaccine strains in a single reaction.
Ameliorative effects of propolis and wheat germ oil on acute toxoplasmosis in experimentally infected mice are associated with reduction in parasite burden and restoration of histopathological changes in the brain, uterus, and kidney
Authors: Elmahallawy Ehab Kotb , Ali Fatma Abo Zakaib , Raya-Álvarez Enrique , Fehaid Alaa , Abd El-Razik Khaled A. , El Fadaly Hassan Ali Mohamed , El-Khadragy Manal F. , Sayed Amal S. M. , Soror Ashraf H. , Alhegaili Alaa S. , Saleh Amira A. , Alkhaldi Abdulsalam A. M. , Madboli Abd El-Nasser A. , Agil Ahmad , Barakat Ashraf Mohamed
Published: March 2024

Toxoplasmosis continues to be a prevalent parasitic zoonosis with a global distribution. This disease is caused by an intracellular parasite known as Toxoplasma gondii, and the development of effective novel drug targets to combat it is imperative. There is limited information available on the potential advantages of wheat germ oil (WGO) and propolis, both individually and in combination, against the acute phase of toxoplasmosis. In this study, acute toxoplasmosis was induced in Swiss albino mice, followed by the treatment of infected animals with WGO and propolis, either separately or in combination. After 10 days of experimental infection and treatment, mice from all groups were sacrificed, and their brains, uteri, and kidneys were excised for histopathological assessment. Additionally, the average parasite load in the brain was determined through parasitological assessment, and quantification of the parasite was performed using Real-Time Polymerase Chain Reaction targeting gene amplification. Remarkably, the study found that treating infected animals with wheat germ oil and propolis significantly reduced the parasite load compared to the control group that was infected but not treated. Moreover, the group treated with a combination of wheat germ oil and propolis exhibited a markedly greater reduction in parasitic load compared to the other groups. Similarly, the combination treatment effectively restored the histopathological changes observed in the brain, uterus, and kidney, and the scoring of these reported lesions confirmed these findings. In summary, the present results reveal intriguing insights into the potential therapeutic benefits of wheat germ oil and propolis in the treatment of acute toxoplasmosis.
What Do You Need? Information Requirements and Task Analysis of (Future) Advanced Air Mobility Pilots in the Emergency Medical Service
Authors: Janetzko Dominik , Kacem Bacem
Published: March 2024

In the domain of Advanced Air Mobility (AAM), Simplified Vehicle Operations (SVOs) promise a reduction in handling complexity and training time for pilots. Designing a usable human–machine interface (HMI) for pilots of SVO-enabled aircraft requires a deep understanding of task and user requirements. This paper describes the results of two user research methods to gather these requirements. First, a traditional Helicopter Emergency Medical Service (HEMS) mission was examined using a Hierarchical Task Analysis (HTA). The findings were used to formulate a theoretical HTA for a single-piloted electric Vertical Take-Off and Landing (eVTOL) system in such a scenario. In the second step, qualitative interviews with seven subject matter experts (pilots and paramedic support) in HEMS operations produced vital user requirements for HMI development. Key findings emphasize the necessity of a simplified information presentation and collision avoidance support in the HMI.
An Experimental and Analytical Approach to Evaluate Transponder-Based Aircraft Noise Monitoring Technology
Authors: Yang Chuyang , Mott John H.
Published: March 2024

Aviation is a vital modern transportation sector connecting millions of passengers globally. Sustainable aviation development holds substantial community benefits, necessitating effective management of its environmental impacts. This paper addresses the need for an accurate and cost-effective aircraft noise monitoring model tailored to non-towered general aviation airports with limited resources for official air traffic data collection. The existing literature highlights a heavy reliance on air traffic data from control facilities in prevailing aircraft noise modeling solutions, revealing a disparity between real-world constraints and optimal practices. Our study presents a validation of a three-stage framework centered on a low-cost transponder unit, employing an innovative experimental and analytical approach to assess the model’s accuracy. An economical Automatic Dependent Surveillance-broadcast (ADS-B) receiver is deployed at Purdue University Airport (ICAO Code: KLAF) to estimate aircraft noise levels using the developed approach. Simultaneously, a physical sound meter is positioned at KLAF to capture actual acoustic noise levels, facilitating a direct comparison with the modeled data. Results demonstrate that the developed noise model accurately identifies aircraft noise events with an average error of 4.50 dBA. This suggests the viability of our low-cost noise monitoring approach as an affordable solution for non-towered general aviation airports. In addition, this paper discusses the limitations and recommendations for future research.
Sizing of Multicopter Air Taxis—Weight, Endurance, and Range
Authors: Yang Yannian , Liang Yu , Pröbsting Stefan , Li Pengyu , Zhang Haoyu , Huang Benxu , Liu Chaofan , Pei Hailong , Noack Bernd R.
Published: March 2024

In the near future, urban air mobility (UAM) will let an old dream of human society come true: affordable and fast air transportation for almost everyone. Among the various existing designs, the multicopter configuration best combines the advantages of compactness, simplicity, and maturity. These aspects are important for actual use, particularly during the early stage of this market. This study elaborates on the design principles of UAM multicopters by examining existing models in terms of their configuration, weight, and range specifications. In particular, the weights of the different components are estimated based on empirical models, aerodynamic fundamentals for the analysis of UAM multicopters are derived from momentum theory, and the power and energy requirements for hovering and cruise flight are evaluated, thereby enabling estimation of the maximum hovering time and flight range. Finally, a sizing method is introduced and validated against an actual UAM design.
A Comprehensive Analysis of Beekeeping Risks and Validation of Biosecurity Measures against Major Infectious Diseases in Apis mellifera in Europe
Authors: De Carolis Alessandra , Newmark Adam J. , Kim Jieun , Song Junxia , Pietropaoli Marco , Manara Veronica , Gyorffy Andrea , Cazier Joseph , Formato Giovanni
Published: March 2024

Background/Objectives: In a collaborative effort, FAO, the International Federation of Beekeepers’ Association “Apimondia”, the Appalachian State University, and the Istituto Zooprofilattico Sperimentale del Lazio e Toscana (IZSLT), the FAO Reference Centre for Animal Health and Food Security Discipline Apiculture, health, and biosecurity, designed an online international survey (“Knowledge, Awareness and Practice”-KAP) as a risk assessment tool to validate biosecurity measures able to prevent and control the main honeybee (Apis mellifera subsp.) infectious diseases. Methods: The KAP survey was disseminated through various channels for 14 months (January 2019–March 2020). Beekeepers’ knowledge on nosemosis, American foulbrood (AFB), and European foulbrood (EFB) management was assessed. Beekeepers’ attitudes were investigated by asking them to provide a score called “Acceptance by beekeepers”. Results: Of the 410 respondents, the majority of them (68.3%, n = 252) were from Europe. European beekeepers (n = 252) considered “Highly acceptable” the following practices: removing combs that show signs of dysentery and feeding colonies for nosemosis; the quick identification and management of affected hives for American foulbrood and European foulbrood. Instead, the following practices were considered as not acceptable by beekeepers: for nosemosis, the use of antibiotics and sampling of hive debris for early detection; for American foulbrood and European foulbrood, the shook swarm, the destruction of the whole apiary, as well as their treatment with antibiotics. Conclusion: This study demonstrated the validity of the present “Knowledge, Awareness and Practice” (KAP) survey to validate the best practices for the main infectious honeybee diseases, considering the pragmatic point of view of beekeepers. Sustainable practices for disease control are preferred by the European beekeepers. While having an adequate knowledge base for the early detection of the investigated honeybee diseases, European beekeepers tend to be aware of the health status of their hives, embracing advanced, practice-oriented training. Authors highlight that the selection of practices and their validation should be revised on a regular basis, to be adapted in a timely manner to the current infectious honeybee disease situation, in order to build up a resilient and effective stock of practices.
Human–Robot Skill Transferring and Inverse Velocity Admittance Control for Soft Tissue Cutting Tasks
Authors: Liu Kaidong , Xie Bin , Chen Zhouyang , Luo Zhenhao , Jiang Shan , Gao Zhen
Published: March 2024

Robotic meat cutting is increasingly in demand in meat industries due to safety issues, labor shortages, and inefficiencies. This paper proposes a multi-demonstration human–robot skill transfer framework to address the flexible and generalized cutting of sheep hindquarters with complex 3D anatomy structures by imitating humans. To improve the generalization with meat sizes and demonstrations and extract target cutting behaviors, multi-demonstrations of cutting are encoded into low-dimension latent space through principal components analysis (PCA), Gaussian mixture model (GMM), and Gaussian mixture regression (GMR). To improve the robotic cutting flexibility and the cutting behavior reproducing accuracy, this study combines a modified dynamic movement primitive (DMP) high-level behavior generator with the low-level joints admittance control (AC) through real-time inverse velocity (IV) kinematics solving and constructs the IVAC-DMP control module. The experimental results show that the maximum residual meat thickness in the sheep hindquarter cutting of sample 1 is 3.1 mm, and sample 2 is 3.8 mm. The residual rates of samples 1 and 2 are 5.6% and 4.8%. Both meet the requirements for sheep hindquarter separation. The proposed framework is advantageous for harvesting high-value meat products and providing a reference technique for robot skill learning in interaction tasks.
Plastic Mulching Effects on Cotton Seedling and Wilt Disease, Lint Yields, and Yield Components
Authors: You Xingmei , Zhang Jingru , Xue Yannan , Zhang Ruikai , Zhang Siwen , Li Chuanwang , Xia Xiaoming
Published: March 2024

Plastic mulching is a widely used intensive planting system for cotton production in China. For the present study, the effects of three plastic mulching treatments (i.e., NDNM: normal sowing date with no plastic mulching as a positive control, NDM: normal sowing date with plastic mulching, and LDM: sowing 7 days late with plastic mulching) were studied in the field on seedling disease, Verticillium wilt, and Fusarium wilt as well as on the lint yield in cotton from 2019 to 2020. The treatment effects were evaluated based on the disease incidence (DI) and disease severity index (DSI), seedling fresh weights, lint yields, and yield components. For all cultivars (SCRC28, SCRC21, and Jimian11), both the DIs and DSIs of the seedling disease were lower in the LDM treatment than in the NDNM and NDM treatments. The DIs and DSIs of Fusarium wilt for all the cultivars were higher in the NDNM treatment than in the NDM and LDM treatments. However, the DIs and DSIs for Verticillium wilt were lower in the NDNM treatment. Moreover, the seedling fresh weights, average lint yields, and boll numbers per square meter were all highest in the NDM treatment and lowest in the NDNM treatment. The results of this study demonstrated that the use of plastic mulching with a suitable seed sowing date would be an appropriate cultural practice for enhancing cotton production and reducing the severity of cotton seedling and Fusarium wilt disease.
Effect of Polymer Adjuvant Type and Concentration on Atomization Characteristics of Nozzle
Authors: Hu Peng , Zhang Ruirui , Chen Liping , Li Longlong , Tang Qing , Yan Wenlong , Yang Jiajun
Published: March 2024

(1) Background: Various types of adjuvants are added during application to enhance the spraying effect, but most adjuvant formulations are proprietary products, and their exact compositions have not been disclosed. (2) Methods: The spatial distributions of droplet sizes and velocities generated by the atomization of different polymer adjuvants were measured based on the phase Doppler interferometer (PDI) measurement method. The sub-area statistical method was used to quantitatively analyze the droplet characteristics at various points below the nozzle. (3) Results: The polymer (polyethylene oxide (PEO))/associative surfactant (sodium dodecyl sulfate (SDS)) can increase the droplet size and velocity of the solution after atomization by increasing the amount of the polymer/associative surfactant to reduce the equilibrium surface tension of the solution and increase the viscosity. Different concentrations of polymer/associative surfactant atomization produced larger droplet sizes and better uniformity than polymer/non-associative surfactant (polysorbate-20 (Tween20)). At the same position, the relationship between droplet velocities for the three adjuvant combinations was roughly as follows: PEO/SDS solution had the highest velocity, followed by PEO solution, and PEO/Tween20 solution had the lowest velocity. (4) Conclusions: The optimal of droplet size distribution can be achieved by adding appropriate amounts of associative surfactants to polymers.
Warming Mitigates the Impacts of Degradation on Nitrogen Allocation between Soil Microbes and Plants in Alpine Meadow
Authors: Pang Zhe , Wen Guoqi , Jiang Lili , Nie Xiaowei , Wang Zongsong , Pang Rui , Liu Wenjing , Chen Meirong , Zhao Weiwai , Tang Li , Zhang Biao , Li Linfeng , Zhou Shutong , Xu Xingliang , Hao Yanbin , Cui Xiaoyong , Wang Shiping , Wang Yanfen
Published: March 2024

In alpine meadows, plants and soil microbes typically engage in competition for nitrogen (N) under N-deficient conditions. However, the acquisition and distribution of N among soil microbes and plants under alpine meadow degradation and climate warming induced by global climate change are still uncharacterized. In this study, we isotope labeled inorganic (NH4+-15N, NO3−-15N) and organic (glycine-15N) N in both degraded and non-degraded plots by using open-top chambers (OTC) to mimic increasing air temperatures. After 6 h, the 15N contents in soil microbes and plants were measured to investigate the effects of degradation and rising air temperature on N allocations in the ecosystems studied. Results showed that alpine meadow degradation significantly reduced soil microbial N accumulation by 52% compared to those in non-degraded plots. In non-degraded plots, warming significantly lowered the organic N levels of soil microbes by 49%, whereas in degraded ones, it reduced both NH4+-15N and NO3−-15N recovery by 80% and 45% on average but increased glycine-15N recovery by 653%. Meanwhile, warming decreased the plant recovery of NH4+-15N and NO3−-15N by 75% and 45% but increased the recovery of glycine-15N by 45% in non-degraded plots. Conversely, in degraded plots, warming markedly lowered NH4+-15N recovery by 40% but increased glycine-15N recovery by 114%. Warming mitigates the effects of alpine meadow degradation on nitrogen allocation among soil microbes and plants. In unwarmed plots, degradation significantly elevated the total 15N recovery ratio of soil microbes to plants by 60%. However, in warmed plots, the impact of degradation on this ratio was reduced. The responses of the 15N recovery ratio of soil microbes and plants to rising temperatures were closely related to alpine meadow quality. In non-degraded areas, warming enhanced the recovery ratio for NH4+-15N by 165% but reduced it for glycine-15N by 66%. Conversely, in degraded plots, warming decreased the recovery ratio for NH4+-15N by 66% but increased it for glycine-15N by 232%. This indicates that warming can increase carbon limitation for soil microbes in degraded alpine meadows, and the restoration of degraded alpine meadows should prioritize restoring carbon accumulation.