bluuebunny/crossref_metadata_2025 huggingface dataset:
Using the LCA Method to Develop the Production of Pigment for Processing Plastics
Authors: Bałdowska-Witos Patrycja , Tomporowski Andrzej , Bieliński Marek
Published: August 2023

In recent years, the chemical industry has been developing more and more dynamically, which results in the introduction of many new chemical substances to the market. However, some of them do not meet the accepted standards and may be toxic to humans and the environment. This problem largely concerns polymer materials, which are currently widely used in many areas of the economy. This is indirectly related to the coloring of these materials during processing. Therefore, it became necessary to introduce modern research procedures that enable the quantitative and qualitative determination of the impact of coloring agents in the processing of plastics, in order to include their negative impact on humans and the natural environment. The LCA methodology was used in this work, with ReCiPe 2016 used as the test method. Among the analyzed technological operations, the highest negative impact on the environment was characterized by the process related to heating the tested material (2.08 × 10−1 Pt). Among the materials, polyethylene terephthalate was distinguished by the greatest harmful effect on human health (2.91 × 10−1 Pt) and the quality (2.35 × 10−2 Pt) of the environment. The use of recycling processes would reduce the negative impact on human health (about −3.71 Pt), the ecosystem (about −0.14 Pt), and resources (about −0.27 Pt).
The Influence of Metakaolin and Polypropylene Fiber Concrete on Mechanics Properties and Microstructure Combined Action under Multi-Salt Soaking and Freeze–Thaw
Authors: Gao Yongwei , Zhou Borui , Yao Xianhua , Guan Junfeng , Han Xiaoyu
Published: August 2023

The wide distribution of alpine saline areas in China is faced with two major problems, which are salt intrusion and freeze–thaw. In total, 216 specimens were prepared with 6 kinds of concrete mix proportions in this paper. The effects of the single and compound incorporation of metakaolin (MK) and polypropylene fiber (PPF) of different amounts on the mechanical properties and microstructure properties of concrete were investigated under the dual action of multi-salt (NaCl, MgCl2, Na2SO4, and NaHCO3) soaking and freeze–thaw. Potable water and freeze–thaw concrete were adopted as the control group. Changes in the appearance morphology, mass loss, relative dynamic elastic modulus, and compressive strength of the concrete were tested, and the microstructure of the concrete was analyzed by scanning electron microscopy (SEM). The results showed that an admixture of both MK and PPF in the potable water and freeze–thaw cycle test can improve the mechanical properties and frost resistance of concrete. The admixture of PPF can effectively improve the mechanical properties and frost resistance of concrete. However, the admixture of MK failed to improve the mechanical properties and frost resistance of concrete during multi-salt soaking and freeze–thaw. The frost resistance of concrete under multi-salt soaking and freeze–thaw was optimally improved with 10% MK and 0.6 kg/m3 PPF. Its microstructure shows that PPF can effectively inhibit crack propagation and reduce the deterioration of concrete under multi-salt soaking and freeze–thaw.
Cetuximab-Conjugated Magnetic Poly(Lactic-co-Glycolic Acid) Nanoparticles for Dual-Targeted Delivery of Irinotecan in Glioma Treatment
Authors: Dash Banendu Sunder , Lu Yu-Jen , Luo Shu-Hui , Chen Jyh-Ping
Published: August 2023

A glioma is the most common malignant primary brain tumor in adults and is categorized according to its growth potential and aggressiveness. Within gliomas, grade 4 glioblastoma remains one of the most lethal malignant solid tumors, with a median survival time less than 18 months. By encapsulating CPT-11 and oleic acid-coated magnetic nanoparticles (OMNPs) in poly(lactic-co-glycolic acid) (PLGA) nanoparticles, we first prepared PLGA@OMNP@CPT-11 nanoparticles in this study. After conjugating cetuximab (CET) with PLGA@OMNP@CPT-11, spherical PLGA@OMNP@CPT-11-CET nanoparticles with 250 nm diameter, 33% drug encapsulation efficiency, and 22% drug loading efficiency were prepared in a single emulsion/evaporation step. The nanoparticles were used for dual-targeted delivery of CPT-11 to U87 primary glioblastoma cells by actively targeting the overexpressed epidermal growth factor receptor on the surface of U87 cells, as well as by magnetic targeting. The physicochemical properties of nanoparticles were characterized in detail. CET-mediated targeting promotes intracellular uptake of nanoparticles by U87 cells, which can release four times more drug at pH 5 than at pH 7.4 to facilitate drug release in endosomes after intracellular uptake. The nanovehicle PLGA@OMNP-CET is cytocompatible and hemocompatible. After loading CPT-11, PLGA@OMNP@CPT-11-CET shows the highest cytotoxicity toward U87 compared with free CPT-11 and PLGA@OMNP@CPT-11 by providing the lowest drug concentration for half-maximal cell death (IC50) and the highest rate of cell apoptosis. In orthotopic brain tumor-bearing nude mice with U87 xenografts, intravenous injection of PLGA@OMNP@ CPT-11-CET followed by guidance with a magnetic field provided the best treatment efficacy with the lowest tumor-associated signal intensity from bioluminescence imaging.
Analysis of U-V2X Communications with Non-Clustered and Clustered Jamming in the Presence of Fluctuating UAV Beam Width
Authors: Arif Mohammad , Kim Wooseong
Published: August 2023

Jammers emit strong intentional jamming signals aiming to limit or block legitimate communications. The distribution of jammers, whether in non-clustered or clustered form, significantly influences the performance of vehicle-to-everything (V2X) networks. In addition, the fluctuations in the three-dimensional (3D) antenna beam width of unmanned aerial vehicles (UAVs) can exert a substantial impact on the network’s overall performance. This paper introduces a model for UAV-V2X (U-V2X) communications in mm-Wave bands, considering non-clustered and clustered jammers, as well as the varying 3D antenna beam width. The roads are modeled using a Poisson line process, vehicular nodes (VNs) are modeled using a 1D Poisson point process (PPP), and UAVs are modeled using a 3D PPP. The jammers are distributed in two ways: non-clustered and clustered distributions. Moreover, the fluctuations in the 3D antenna beam width follow a normal distribution. To this end, a typical node’s performance in U-V2X communications is evaluated for various network configurations, including the number of UAVs, VNs, roads, jammers, and jammer’s transmission power. The analytical expressions for the outage probability (OP) of VN to VN connection (i.e., V2V), VN to UAV connection (i.e., V2U2V), and an overall connection (i.e., U-V2X), under non-clustered and clustered jamming, along with the fluctuating antenna beam width, are derived. The results revealed that the performance of the U-V2X communications utilizing mm-Waves is significantly degraded with the non-clustered jamming in comparison with the clustered jamming. The fluctuations in the 3D beam width of the UAV antennas further compromise the network’s performance. Thus, accurate modeling of these fluctuations is crucial, particularly in the presence of non-clustered jammers. Furthermore, the system designers should focus on implementing additional anti-jamming countermeasures specifically targeting non-clustered jammers in U-V2X communications.
Fixed Point Results in Controlled Fuzzy Metric Spaces with an Application to the Transformation of Solar Energy to Electric Power
Authors: Ishtiaq Umar , Kattan Doha A. , Ahmad Khaleel , Sessa Salvatore , Ali Farhan
Published: August 2023

In this manuscript, we give sufficient conditions for a sequence to be Cauchy in the context of controlled fuzzy metric space. Furthermore, we generalize the concept of Banach’s contraction principle by utilizing several new contraction conditions and prove several fixed point results. Furthermore, we provide a number of non-trivial examples to validate the superiority of main results in the existing literature. At the end, we discuss an important application to the transformation of solar energy to electric power by utilizing differential equations.
Optimal Control Design and Online Controller-Area-Network Bus Data Analysis for a Light Commercial Hybrid Electric Vehicle
Authors: Babangida Aminu , Light Odazie Chiedozie Maduakolam , Szemes Péter Tamás
Published: August 2023

In this article, a hybrid powertrain for the Volkswagen (VW) Crafter is designed using the Model-In-The-Loop (MIL) method. An enhanced Proportional-Integral (PI) control technique based on integral cost functions is developed by carrying out a time-based simulation in MATLAB/Simulink software to realize the optimal fuel economy of the vehicle. Moreover, a comparative study is conducted between the vehicle’s hybrid and pure electric versions to assess the optimal battery energy consumption per unit distance traveled. Communication within our vehicles’ Electronic Control Units (ECUs) is facilitated by a message-based protocol called a Controller Area Network (CAN). Consequently, this paper presents an online CAN Bus data analysis using the Hardware-In-The-Loop (HIL) method. This method uses a standard frame, J1939 CAN protocol, implemented with Net CAN Plus 110 hardware. A graphical user interface is developed on a host Personal Computer (PC) using LabVIEW for decoding the acquired raw CAN data to physical values. The simulation results reveal that the proposed controller is promising and suitable for realizing optimal performance over the HIL method.
A Study of Independency on Fuzzy Resolving Sets of Labelling Graphs
Authors: Shanmugapriya Ramachandramoorthi , Hemalatha Perichetla Kandaswamy , Cepova Lenka , Struz Jiri
Published: August 2023

Considering a fuzzy graph G is simple and can be connected and considered as a subset H=u1,σu1,u2,σu2,…uk,σuk, |H|≥2; then, every two pairs of elements of σ−H have a unique depiction with the relation of H, and H can be termed as a fuzzy resolving set (FRS). The minimal H cardinality is regarded as the fuzzy resolving number (FRN), and it is signified by FrG. An independence set is discussed on the FRS, fuzzy resolving domination set (FRDS), and Fuzzy modified antimagic resolving set (FMARS). In this paper, we discuss the independency of FRS and FMARS in which an application has also been developed.
Limited Palatal Muscle Resection for the Treatment of Obstructive Sleep Apnea: A Systematic Review and Meta-Analysis
Authors: Park Marn , Lee Young-Ha , Cho Jae , Choi Ji
Published: August 2023

Background and Objectives: Limited palatal muscle resection (PMR) is a surgical technique employed to alleviate respiratory disturbances in obstructive sleep apnea (OSA) patients with retropalatal narrowing by reducing soft palate volume and tightening the muscles. Although some previous publications have demonstrated the effectiveness of limited PMR, the overall efficacy and therapeutic role of limited PMR for the treatment of OSA remain uncertain. This study utilized meta-analysis and a systematic literature review to estimate the overall effectiveness of limited PMR in treating OSA. Materials and Methods: Multiple databases, including PubMed, EMBASE, Cochrane Library, and Web of Science, were searched using specific keywords related to OSA and limited PMR. Original articles assessing respiratory disturbances before and after limited PMR in patients with OSA were included. Data from selected articles were collected using standardized forms, including clinicodemographic characteristics, apnea-hypopnea index (AHI), and lowest pulse oximetry values (minimum SpO2). Random effect models were used for analyzing significant heterogeneity. Egger’s test and funnel plot were used to identify publication bias. Results: Four studies were included in this meta-analysis for AHI, and three studies were included for minimum SpO2 during sleep. A significant reduction in the AHI and an increase in the minimum SpO2 were shown following limited PMR as the standardized mean difference (95% confidence interval) was 2.591 (1.092–4.090) and 1.217 (0.248–2.186), respectively. No publication bias was found in either analysis. Conclusions: The results of the meta-analysis and systemic review add to the literature that limited PMR can result in a reduction in the AHI and an increase in min SaO2. In OSA patients with suspected retropalatal obstruction, limited PMR may be efficiently performed.
Metabolic Associated Fatty Liver Disease (MAFLD) and COVID-19 Infection: An Independent Predictor of Poor Disease Outcome?
Authors: Milivojević Vladimir , Bogdanović Jelena , Babić Ivana , Todorović Nevena , Ranković Ivan
Published: August 2023

Background and Objectives: Early reports on COVID-19 infection suggested that the SARS-CoV-2 virus solely attacks respiratory tract cells. As the pandemic spread, it became clear that the infection is multiorganic. Metabolic associated fatty liver disease (MAFLD) is a chronic liver disease strongly associated with insulin resistance and diabetes. The aim of this study was to assess a possible interplay between MAFLD and COVID-19 infection and its implication in COVID-19 outcome. Materials and Methods: A retrospective observational study, including 130 COVID-19 positive patients was conducted. MAFLD diagnosis was made based on the International Consensus criteria. Patients were divided into two groups, group A (MAFLD) and group B (nonMAFLD). Anthropometric and laboratory analysis were obtained. COVID-19 severity was assessed using the NEWS2 score. Disease outcome was threefold and regarded as discharged, patients who required mechanical ventilation (MV), and deceased patients. Results: MAFLD prevalence was 42%, 67% of patients were discharged, and 19% needed MV. Mortality rate was 14%. MAFLD patients were significantly younger (p < 0.001), and had higher body mass index (p < 0.05), respiratory rate (p < 0.05) and systolic blood pressure (p < 0.05) than nonMAFLD patients. Regarding metabolic syndrome and inflammatory markers: group A had significantly higher glycemia at admission (p = 0.008), lower HDL-c (p < 0.01), higher triglycerides (p < 0.01), CRP (p < 0.001), IL-6 (p < 0.05) and ferritin (p < 0.05) than group B. MAFLD was associated with more prevalent type 2 diabetes (p = 0.035) and hypertension (p < 0.05). MAFLD patients had a more severe disease course (NEWS2 score, 6.5 ± 0.5 vs. 3 ± 1.0, p < 0.05). MAFLD presence was associated with lower patient discharge (p < 0.01) and increased need for MV (p = 0.024). Multiple regression analysis showed that BMI (p = 0.045), IL-6 (p = 0.03), and MAFLD (p < 0.05) are significant independent risk factors for a poor COVID-19 outcome. Conclusions: The prevalence of MAFLD is relatively high. MAFLD patients had a more severe COVID-19 clinical course and worse disease outcome. Our results imply that early patient stratification and risk assessment are mandatory in order to avoid poor outcomes.
Electrostatic Potentials Caused by the Release of Protons from Photoactivated Compound Sodium 2-Methoxy-5-nitrophenyl Sulfate at the Surface of Bilayer Lipid Membrane
Authors: Sokolov Valerij S. , Tashkin Vsevolod Yu. , Zykova Darya D. , Kharitonova Yulia V. , Galimzyanov Timur R. , Batishchev Oleg V.
Published: August 2023

Lateral transport and release of protons at the water–membrane interface play crucial roles in cell bioenergetics. Therefore, versatile techniques need to be developed for investigating as well as clarifying the main features of these processes at the molecular level. Here, we experimentally measured the kinetics of binding of protons released from the photoactivated compound sodium 2-methoxy-5-nitrophenyl sulfate (MNPS) at the surface of a bilayer lipid membrane (BLM). We developed a theoretical model of this process describing the damage of MNPS coupled with the release of the protons at the membrane surface, as well as the exchange of MNPS molecules and protons between the membrane and solution. We found that the total change in the boundary potential difference across the membrane, ∆ϕb, is the sum of opposing effects of adsorption of MNPS anions and release of protons at the membrane–water interface. Steady-state change in the ∆ϕb due to protons decreased with the concentration of the buffer and increased with the pH of the solution. The change in the concentration of protons evaluated from measurements of ∆ϕb was close to that in the unstirred water layer near the BLM. This result, as well as rate constants of the proton exchange between the membrane and the bulk solution, indicated that the rate-limiting step of the proton surface to bulk release is the change in the concentration of protons in the unstirred layer. This means that the protons released from MNPS remain in equilibrium between the BLM surface and an adjacent water layer.
Iron Control in Liquid Effluents: Pseudo-Emulsion Based Hollow Fiber Membrane with Strip Dispersion Technology with Pseudo-Protic Ionic Liquid (RNH3+HSO4−) as Mobile Carrier
Authors: Alguacil Francisco Jose , Robla Jose Ignacio
Published: August 2023

The transport of iron(III) from aqueous solutions through pseudo-emulsion-based hollow fiber with strip dispersion (PEHFSD) was investigated using a microporous hydrophobic hollow fiber membrane module. The pseudo-protic ionic liquid RNH3HSO4− dissolved in Solvesso 100 was used as the carrier phase. This pseudo-protic ionic liquid was generated by the reaction of the primary amine Primene JMT (RNH2) with sulphuric acid. The aqueous feed phase (3000 cm3) containing iron(III) was passed through the tube side of the fiber, and the pseudo-emulsion phase of the carrier phase (400 cm3) and sulphuric acid (400 cm3) were circulated through the shell side in counter-current operational mode, using a single hollow fiber module for non-dispersive extraction and stripping. In the operation, the stripping solution (sulphuric acid) was dispersed into the organic membrane phase in a tank with a mixing arrangement (a four-blade impeller stirrer) designed to provide strip dispersion. This dispersed phase was continuously circulated from the tank to the membrane module in order to provide a constant supply of the organic solution to the fiber pores. Different hydrodynamic and chemical parameters, such as feed (75–400 cm3/min) and pseudo-emulsion phases (50–100 cm3/min) flows, sulphuric acid concentration in the feed and stripping phases (0.01–0.5 M and 0.5–3 M, respectively), metal concentration (0.01–1 g/L) in the feed phase, and PPILL concentration (0.027–0.81 M) in the carrier phase, were investigated. From the experimental data, different diffusional parameters were estimated, concluding that the resistance due to the feed phase was not the rate-controlling step of the overall iron(III) transport process. It was possible to concentrate iron(III) in the strip phase using this smart PEHFSD technology.
Influence of Current Density upon Hydrogenation on the Shape Memory Effect of Binary TiNi Alloy Single Crystals
Authors: Kireeva Irina V. , Chumlyakov Yuriy I. , Yakovleva Liya P. , Vyrodova Anna V.
Published: August 2023

Some results concerning the hydrogen effect at electrolytic saturation at a current density of j = 1500 and 3500 A/m2 for 3 h at room temperature on the temperature dependence of the yield stress σ0.1(T) and the shape memory effect (SME) under tension of the [011]-oriented Ti-50.55%Ni (at.%) alloy single crystals are presented. It was shown that hydrogen is in a solid solution and forms particles of titanium hydride TiH2 after hydrogenation at j = 1500 and 3500 A/m2, respectively. Both hydrogen in the solid solution and TiH2 particles led to a decrease in the Ms temperature of the onset of the forward martensitic transformation (MT) upon cooling and the Md temperature (Md is the temperature at which the stresses for the onset of the stress-induced MT are equal to the stresses for the onset of plastic flow of the high-temperature B2 phase), and increased the yield stress σ0.1 of the B2 phase at the Md temperature compared to hydrogen-free crystals. It was found that the SME under stress depends on the tensile stress level and current density. The maximum SME εSME = 10 ± 0.2% at σex = 200 MPa and εSME = 10.5 ± 0.2% at σex = 300 MPa was observed in the hydrogen-free crystals and after hydrogenation at j = 1500 A/m2, respectively, which exceeded the theoretical value of lattice deformation ε0 = 8.95% for the B2-B19′ MT in [011] orientation under tension. At j = 1500 A/m2, the physical reason for the excess of the SME of the theoretical ε0 value was due to the increase in the plasticity of B19′ martensite upon hydrogenation. At j = 3500 A/m2, εSME = 8.0 ± 0.2%, and it was less than ε0 = 8.95% for B2-B19′ MT in [011] orientation under tension. The decrease in SME after hydrogenation at j = 3500 A/m2 was associated with the interaction of two types of B19′-martensite: oriented under stress and non-oriented, formed near TiH2 particles. It was shown that the redistribution of hydrogen in the bulk of the crystals during long-term holding for 168 h at 263 K after hydrogenation at j = 1500 A/m2 increases the SME relative to crystals without long-term holding: 3.5 times at 50 MPa and 1.8 times at 100–150 MPa. After long-term holding, εSME = 9.5 ± 0.2% at 150 MPa, which exceeds the theoretical value ε0 = 8.95% for B2-B19′ MT in [011] orientation under tension.
Effect of Microstructure on Coalescence-Induced Droplet Jumping Behavior of a Superhydrophobic Surface and Its Application for Marine Atmospheric Corrosion Protection
Authors: Chen Zhengshen , Chen Xiaotong , Sun Yihan , Wang Guoqing , Wang Peng
Published: August 2023

Coalescence-induced droplet jumping behavior (CIDJB) refers to the spontaneous jumping of droplets on a specific superhydrophobic surface (SS) without any external energy, which offers a new approach to the field of marine atmospheric corrosion protection by isolating corrosive media. In this study, a flower-like micro–nanocomposite structure SS (F-SS) and a sheet-like nanostructure SS (S-SS) were prepared on copper sheets by ammonia immersion and chemical vapor deposition. Firstly, we observed the microstructure characteristics of the samples and secondly analyzed its chemical composition and wettability. Moreover, the CIDJB was studied by simulated condensation experiments, and the influence of the microstructure on CIDJB was revealed. Meanwhile, the atmospheric corrosion resistance of samples was analyzed by electrochemical impedance spectroscopy (EIS) measurements, and the protection mechanism of SS through CIDJB was proposed. The results showed that the S-SS had a smaller solid–liquid contact area and lower interfacial adhesion, which is more conducive to CIDJB. Since a larger solid–liquid contact area requires greater interface adhesion energy for the droplets to overcome, droplet jumping behavior was not observed on the F-SS. Compared with the F-SS, the S-SS exhibited outstanding corrosion resistance due to the wettability transition of droplets by CIDJB, which facilitated the restoration of the air film to insulate the corrosive medium. The present study provides a reference for a marine atmospheric corrosion resistance technique through CIDJB on an SS.
Two-Step Spin Forming of Thin-Walled Heads with Lateral Normal Flanged Holes
Authors: Zhu Lijun , Huang Cheng , Li Xinhe , Chang Xin , Liu Chao
Published: August 2023

A thin-walled head with a lateral normal flanged hole is a key part of a propellant tank, and its low-cost and high-precision forming process is very challenging. In this paper, a two-step method is proposed to preform the head via marginal-restraint mandrel-free spinning and then to realize the flanging of the lateral normal hole using a punching–spinning method. Finite element analysis of round-hole punching–spinning flanging shows that the larger the thinning ratio and the roller fillet radius, the lower the rebound and contour rise amount; the larger the feed ratio, the lower the rebound and the higher the contour rise amount. Further study on the effects of round-hole punching–spinning flanging on the secondary deformation of thin-walled heads shows that the deformation of the head in the area around the flanging hole is less severe than that of the flat blank, and the deformation in the circumferential direction is different from that in the busbar direction. Finally, it is verified through experiments that the two-step method can realize the spin forming of thin-walled heads with lateral normal flanged holes.
Statistical Analysis of Tribological Properties of Mg(AM50)/GNF-Al2O3sf Hybrid Composites
Authors: Lee Min-Sik , Kang Chung-Gil , Babu J. S. S.
Published: August 2023

The present article describes the tribological properties of Mg-based hybrid composites reinforced with graphite nanofiber (GNF) and alumina short fiber (Al2O3sf) that were investigated. The Mg/GNF/Al2O3sf hybrid composites with varying volume fraction of fiber (10 vol.%, 15 vol.%, 20 vol.%) were developed. SEM observations indicate that the GNF cluster distributions within the array of the Al2O3sf network are found to be relatively good. The Taguchi design of the experiment has been applied to conduct the wear test, and the statistical analysis of variance (ANOVA) has been used to evaluate the influence of wear test parameters on the wear loss and coefficient of friction (COF) of the composites. The influence of wear test parameters such as volume fraction of fiber (VF), applied load (AL), sliding distance (SD), and sliding speed (SP) on the wear loss and COF of composites was analyzed under dry sliding conditions. The results of ANOVA indicate that the sliding distance was found to be the prominent factor affecting wear loss, and the applied load influenced the COF most significantly. Furthermore, the composites with 20 vol.% of fiber had lower wear loss than those with 10 vol.% and 15 vol.% of fiber. The COF of composites with 15 vol.% of fiber was found to be slightly lower compared to the 10 vol.% and 20 vol.% of fiber cases. The results imply that the hybridization of GNFs and Al2O3sf, as well as the formation of Mg17Al12 and Al2MgC2 precipitates enhanced the tribological properties of the Mg hybrid composites.
Natural Grass Cultivation Management Improves Apple Fruit Quality by Regulating Soil Mineral Nitrogen Content and Carbon–Nitrogen Metabolism
Authors: Yu Bo , Wang Lixia , Zhang Jiaqi , Lyu Deguo
Published: August 2023

Orchard grass cultivation management is an effective measure to safeguard the sustainable development of the fruit industry in China. However, details of the influence of natural sod culture management on carbon (C)–nitrogen (N) nutrition of trees and fruit quality in Hanfu apple orchards are lacking. Therefore, a field experiment was conducted, which consisted of two treatments: clean tillage (CT) and natural grass cultivation (NG). Results revealed that NG treatment contributed to the increases in soil organic matter (SOM), total N, and soil NH4+-N at depths of 0–20 cm and 20–40 cm, while the soil NO3−-N concentration under NG treatment was significantly decreased at the same depth, within the range of 0–200 cm of the soil profile, compared with CT. NG treatment also significantly promoted leaf photosynthesis and enhanced leaf N and fruit sugar metabolism. The results of isotope labeling showed that NG treatment obviously elevated the 13C accumulation and distribution rate in fruits, as well as the 15N accumulation in the whole tree, whereas the 15N accumulation in fruits decreased. Furthermore, NG treatment significantly increased the fruit anthocyanin content. These results provide theoretical references for the feasibility of natural sod culture management to improve fruit quality in Hanfu apple orchards.
Resting Metabolic Rate of Individuals
Authors: Plaza-Florido Abel , Alcantara Juan M. A.
Published: August 2023

The resting metabolic rate (RMR) represents the energy required to sustain normal body functions and homeostasis in an awake individual under ambient thermoneutral conditions and during rest [...]
Transcriptome Profiling Based at Different Time Points after Hatching Deepened Our Understanding on Larval Growth and Development of Amphioctopus fangsiao
Authors: Li Zan , Bao Xiaokai , Liu Xiumei , Wang Weijun , Yang Jianmin , Zhu Xibo , Wang Shuhai
Published: August 2023

As the quality of life improves, there is an increasing demand for nutrition-rich marine organisms like fish, shellfish, and cephalopods. To address this, artificial cultivation of these organisms is being explored along with ongoing research on their growth and development. A case in point is Amphioctopus fangsiao, a highly valued cephalopod known for its tasty meat, nutrient richness, and rapid growth rate. Despite its significance, there is a dearth of studies on the A. fangsiao growth mechanism, particularly of its larvae. In this study, we collected A. fangsiao larvae at 0, 4, 12, and 24 h post-hatching and conducted transcriptome profiling. Our analysis identified 4467, 5099, and 4181 differentially expressed genes (DEGs) at respective intervals, compared to the 0 h sample. We further analyzed the expression trends of these DEGs, noting a predominant trend of continuous upregulation. Functional exploration of this trend entailed GO and KEGG functional enrichment along with protein–protein interaction network analyses. We identified GLDC, DUSP14, DPF2, GNAI1, and ZNF271 as core genes, based on their high upregulation rate, implicated in larval growth and development. Similarly, CLTC, MEF2A, PPP1CB, PPP1R12A, and TJP1, marked by high protein interaction numbers, were identified as hub genes and the gene expression levels identified via RNA-seq analysis were validated through qRT-PCR. By analyzing the functions of key and core genes, we found that the ability of A. fangsiao larvae to metabolize carbohydrates, lipids, and other energy substances during early growth may significantly improve with the growth of the larvae. At the same time, muscle related cells in A. fangsiao larvae may develop rapidly, promoting the growth and development of larvae. Our findings provide preliminary insights into the growth and developmental mechanism of A. fangsiao, setting the stage for more comprehensive understanding and broader research into cephalopod growth and development mechanisms.
Effect of Noise and Music on Neurotransmitters in the Amygdala: The Role Auditory Stimuli Play in Emotion Regulation
Authors: Nian Haoyang , Ding Susu , Feng Yanru , Liu Honggui , Li Jianhong , Li Xiang , Zhang Runxiang , Bao Jun
Published: August 2023

Stress caused by noise is becoming widespread globally. Noise may lead to deafness, endocrine disorders, neurological diseases, and a decline in mental health. The mechanism behind noise-induced neurodevelopmental abnormalities is unclear, but apoptosis and pro-inflammatory signals may play an important role. In this study, weaned piglets were used as a model to explore noise-induced neurodevelopmental abnormalities. We hypothesized that long-term noise exposure would induce anxiety and cause acute stress, exhibited by alterations in neurotransmission in the amygdala. A total of 72 hybrid piglets (Large White × Duroc × Min Pig) were randomly divided into three groups, including noise (exposed to mechanical noise, 80–85 dB), control (blank, exposed to natural background sound, <40 dB), and music (positive control, exposed to Mozart K.448, 60–70 dB) groups. The piglets were exposed to 6 h of auditory noise daily (10:00–16:00) for 28 days. Compared with the control group, piglets exposed to noise showed more aggressive behavior. The expression of Caspase3, Caspase9, Bax, NF-κB (p56), TLR4, MYD88, I κ B α, IL-1 β, TNF-α, and IL-12RB2 was significantly upregulated in the amygdala, while the expression of Nrf2, HO-1, CAT, and SOD was downregulated in piglets in the noise group. Cell death occurred, and numerous inflammatory cells accumulated in the amygdala of piglets in the noise group. Targeted metabolomics showed that the content of inhibitory neurotransmitter GABA was higher in the amygdala of piglets in the noise group. Compared with the noise group, piglets in the music group displayed more positive emotion-related behaviors. Compared with the noise group, the expression of genes related to apoptosis, inflammation, and oxidative damage was lower in the music group. Cells of the amygdala in the music group were also of normal morphology. Our results show that noise-induced stress causes apoptosis and neuroinflammation in the amygdala and induces anxiety during the early neonatal neural development of piglets. In contrast, to some extent, music alleviates noise-induced anxiety.
Exhaled Nitric Oxide and Pulmonary Oxygen Toxicity Susceptibility
Authors: Fothergill David M. , Gertner Jeffery W.
Published: August 2023

Individual susceptibility to pulmonary oxygen toxicity (PO2tox) is highly variable and currently lacks a reliable biomarker for predicting pulmonary hyperoxic stress. As nitric oxide (NO) is involved in many respiratory system processes and functions, we aimed to determine if expired nitric oxide (FENO) levels can provide an indication of PO2tox susceptibility in humans. Eight U.S. Navy-trained divers volunteered as subjects. The hyperoxic exposures consisted of six- and eight-hour hyperbaric chamber dives conducted on consecutive days in which subjects breathed 100% oxygen at 202.65 kPa. Subjects’ individual variability in pulmonary function and FENO was measured twice daily over five days and compared with their post-dive values to assess susceptibility to PO2tox. Only subjects who showed no decrements in pulmonary function following the six-hour exposure conducted the eight-hour dive. FENO decreased by 55% immediately following the six-hour oxygen exposure (n = 8, p < 0.0001) and by 63% following the eight-hour exposure (n = 4, p < 0.0001). Four subjects showed significant decreases in pulmonary function immediately following the six-hour exposure. These subjects had the lowest baseline FENO, had the lowest post-dive FENO, and had clinical symptoms of PO2tox. Individuals with low FENO were the first to develop PO2tox symptoms and deficits in pulmonary function from the hyperoxic exposures. These data suggest that endogenous levels of NO in the lungs may protect against the development of PO2tox.
Structural Design and Simulation of a Multi-Channel and Dual Working Condition Wafer Defect Inspection Prototype
Authors: Ding Ruizhe , Luo Haiyan , Li Zhiwei , Zhou Zuoda , Qu Dingjun , Xiong Wei
Published: August 2023

Detecting and classifying defects on unpatterned wafers is a key part of wafer front-end inspection. Defect inspection schemes vary depending on the type and location of the defects. In this paper, the structure of the prototype is designed to meet the requirements of wafer surface and edge defect inspection. This prototype has four inspection channels: scattering, reflection, phase, and contour, with two working conditions: surface and edge inspection. The key structure of the prototype was simulated using Ansys. The simulation results show that the maximum deformation of the optical detection subsystem is 19.5 μm and the fundamental frequency of the prototype is 96.9 Hz; thus, these results meet the requirements of optical performance stability and structural design. The experimental results show that the prototype meets the requirements of the inspection sensitivity better than 200 nm equivalent PSL spherical defects.
An 8–18 GHz 90° Switched T-Type Phase Shifter
Authors: Zeng Jialong , Ren Yuxin , Tan Cheng , Yuan Yang , Li Jiaxuan , Yu Zhongjun
Published: August 2023

This paper proposes a novel 8–18 GHz 90° switched T-type phase shifter (TPS). In contrast to the conventional TPS, the proposed TPS adds a compensation capacitance to greatly enhance the phase shifting capacity. Moreover, the designed structure also integrates a filtering compensation network, which can effectively achieve a flat relative phase shift in a wide band. The proposed 90° TPS is fabricated using 0.15 μm GaAs pHEMT technology. The TPS achieves homogeneous phase shift at 8–18 GHz, with the measured phase error of less than ±1.5°. The insertion loss of the proposed phase shifter is 1.3–2.6 dB, and the chip size is merely 0.53 × 0.86 mm2. Thanks to these excellent performance characteristics, the designed phase shifter is well-suited for ultra-wideband wireless communication and radar systems.
Continuous Flow Separation of Live and Dead Cells Using Gravity Sedimentation
Authors: Ozcelik Adem , Gucluer Sinan , Keskin Tugce
Published: August 2023

The separation of target cell species is an important step for various biomedical applications ranging from single cell studies to drug testing and cell-based therapies. The purity of cell solutions is critical for therapeutic application. For example, dead cells and debris can negatively affect the efficacy of cell-based therapies. This study presents a cost-effective method for the continuous separation of live and dead cells using a 3D resin-printed microfluidic device. Saccharomyces cerevisiae yeast cells are used for cell separation experiments. Both numerical and experimental studies are presented to show the effectiveness of the presented device for the isolation of dead cells from cell solutions. The experimental results show that the 3D-printed microfluidic device successfully separates live and dead cells based on density differences. Separation efficiencies of over 95% are achieved at optimum flow rates, resulting in purer cell populations in the outlets. This study highlights the simplicity, cost-effectiveness, and potential applications of the 3D-printed microfluidic device for cell separation. The implementation of 3D printing technology in microfluidics holds promise for advancing the field and enabling the production of customized devices for biomedical applications.
A Comparative Prospective Study in Evaluating Candida spp. In Vitro Susceptibility through Micronaut-AM and Sensititre Yeast-One
Authors: Trovato Laura , Calvo Maddalena , Scalia Guido , Oliveri Salvatore
Published: August 2023

Background. Among invasive fungal infection pathogens, Candida spp. represent the most common aetiological agents. The increasing rate of severe infections and the emergence of antimicrobial resistance highlight the importance of in vitro susceptibility testing. The EUCAST and the CLSI have established reference microdilutions that are reliable but difficult to apply in a laboratory routine. Commercial microdilutions could represent a valuable alternative within a diagnostic workflow. Methods. A number of 50 Candida spp. collected from positive blood samples simultaneously underwent the Sensititre Yeast-One microdilution as a standard susceptibility test and the Micronaut-AM as an experimental method. A comparison between the two techniques was produced, evaluating the effectiveness of the Micronaut-AM compared to the extensively consolidated Sensititre Yeast-One. Results. The two techniques revealed optimal agreement rates, confirming the reliability of the commercial microdilution kits within the diagnostic workflows. The results showed remarkable concordance for both susceptible and resistant isolates, highlighting slight variations in the different identified Candida species. Conclusions. Future studies about antifungal susceptibility testing should be encouraged, including molecular confirmation of possible resistance phenotypes and extended isolate numbers for the different Candida species. Moreover, it would be interesting to plan clinical trials after the execution of the examined commercial microdilution methods.
Sage and Lavender Essential Oils as Potential Antimicrobial Agents for Foods
Authors: Speranza Barbara , Guerrieri Angela , Racioppo Angela , Bevilacqua Antonio , Campaniello Daniela , Corbo Maria Rosaria
Published: August 2023

Modern society is becoming more and more reluctant to use antibiotic or chemical compounds in food production and is demanding foods without what they perceive as artificial and harmful chemicals, including many used as antimicrobials and preservatives in food. Another big problem is the improper use of antibiotics, especially broad-spectrum ones, which has significantly contributed to increased antibiotic resistance in many microorganisms. As a consequence, the whole scientific world has recently concentrated numerous studies on the research of natural remedies capable of counteracting multidrug-resistant strains and fighting infections: the use of aromatic plants and their essential oils (EOs) as potential alternatives to conventional antimicrobials to extend shelf life and combat foodborne pathogens has heightened. Among EOs, sage and lavender have also been promoted for their potential antimicrobial capabilities. In this review, we summarize the latest research studies performed about sage and lavender EOs, focusing on their chemical composition and their biological and antimicrobial properties; the aim is to give an overview of the current knowledge about their major components, effectiveness, mechanisms of action, synergistic effects and use in foods to facilitate a widespread application in both food and pharmaceuticals industries.
Vaginal Microbial Network Analysis Reveals Novel Taxa Relationships among Adolescent and Young Women with Incident Sexually Transmitted Infection Compared with Those Remaining Persistently Negative over a 30-Month Period
Authors: Mehta Supriya D. , Agingu Walter , Zulaika Garazi , Nyothach Elizabeth , Bhaumik Runa , Green Stefan J. , van Eijk Anna Maria , Otieno Fredrick O. , Phillips-Howard Penelope A. , Schneider John
Published: August 2023

A non-optimal vaginal microbiome (VMB) is typically diverse with a paucity of Lactobacillus crispatus and is often associated with bacterial vaginosis (BV) and sexually transmitted infections (STIs). Although compositional characterization of the VMB is well-characterized, especially for BV, knowledge remains limited on how different groups of bacteria relate to incident STIs, especially among adolescents. In this study, we compared the VMB (measured via 16S ribosomal RNA gene amplicon sequencing) of Kenyan secondary school girls with incident STIs (composite of chlamydia, gonorrhea, and trichomoniasis) to those who remained persistently negative for STIs and BV over 30 months of follow-up. We applied microbial network analysis to identify key taxa (i.e., those with the greatest connectedness in terms of linkages to other taxa), as measured by betweenness and eigenvector centralities, and sub-groups of clustered taxa. VMB networks of those who remained persistently negative reflected greater connectedness compared to the VMB from participants with STI. Taxa with the highest centralities were not correlated with relative abundance and differed between those with and without STI. Subject-level analyses indicated that sociodemographic (e.g., age and socioeconomic status) and behavioral (e.g., sexual activity) factors contribute to microbial network structure and may be of relevance when designing interventions to improve VMB health.
Gut Microbial and Associated Metabolite Markers for Colorectal Cancer Diagnosis
Authors: Alhhazmi Areej A. , Alhamawi Renad M. , Almisned Reema M. , Almutairi Hanouf A. , Jan Ahdab A. , Kurdi Shahad M. , Almutawif Yahya A. , Mohammed-Saeid Waleed
Published: August 2023

Globally, colorectal cancer (CRC) is the second most common cause of mortality worldwide. Considerable evidence indicates that dysbiosis of the gut microbial community and its metabolite secretions play a fundamental role in advanced adenoma (ADA) and CRC development and progression. This study is a systematic review that aims to assess the clinical association between gut microbial markers and/or gut and circulating metabolites with ADA and CRC. Five electronic databases were searched by four independent reviewers. Only controlled trials that compared ADA and/or CRC with healthy control (HC) using either untargeted (16s rRNA gene or whole genome sequencing) or targeted (gene-based real-time PCR) identification methods for gut microbiome profile, or untargeted or targeted metabolite profiling approaches from the gut or serum/plasma, were eligible. Three independent reviewers evaluated the quality of the studies using the Cochrane Handbook for Systematic Reviews of Interventions. Twenty-four studies were eligible. We identified strong evidence of two microbial markers Fusobacterium and Porphyromonas for ADA vs. CRC, and nine microbial markers Lachnospiraceae-Lachnoclostridium, Ruminococcaceae-Ruminococcus, Parvimonas spp., Parvimonas micra, Enterobacteriaceae, Fusobacterium spp., Bacteroides, Peptostreptococcus-Peptostreptococcus stomatis, Clostridia spp.-Clostridium hylemonae, Clostridium symbiosum, and Porphyromonas-Porphyromonas asaccharolytica for CRC vs. HC. The remaining metabolite marker evidence between the various groups, including ADA vs. HC, ADA vs. HC, and CRC vs. HC, was not of sufficient quality to support additional findings. The identified gut microbial markers can be used in a panel for diagnosing ADA and/or CRC. Further research in the metabolite markers area is needed to evaluate the possibility to use in diagnostic or prognostic markers for colorectal cancer.
Color Origin of Greyish-Purple Tremolite Jade from Sanchahe in Qinghai Province, NW China
Authors: Gong Nina , Wang Chaowen , Xu Shuai
Published: August 2023

Greyish-purple tremolite jade has become well known in the past few years, and the origin of its color has attracted the attention of gemologists. In this study, FT-IR spectra, EPMA, EPR spectra, micro-XRF, UV–Vis–NIR spectra, and LA-ICP-MS in situ mapping were analyzed to investigate the chromophore elements. The study sample was chosen from the Sanchahe mine, Qinghai Province, NW China, which has the typical characteristics of a gradual color change. The FT-IR and EPMA results revealed that the mineral composition of the dark and light greyish-purple regions of the sample are primarily composed of tremolite. UV–Vis–NIR spectra demonstrated that the greyish-purple color is mainly due to strong absorptions at 560 nm and 700 nm and weak absorption at 745 nm in the visible range. The EPR spectra presented ~3400 G six hyperfine lines resulting from the hyperfine interactions of the unpaired electron with the Mn2+ nucleus in the octahedral site. The UV–Vis–NIR and EPR spectra analyses demonstrated that Mn2+ is the origin of the purple color. A comparison of the major elements in the light and dark regions indicated that the chromogenic elements have strong positive correlations with Mn, Cu, and Fe. LA-ICP-MS mapping used to analyze the first transition metals indicated possible positive correlations between the greyish-purple color and the trace chromogenic elements. This suggested that the Mn, Cu, and Fe contents are significantly high in the dark band region. Combining in situ LA-ICP-MS mapping of trace elements, UV–Vis spectra, and EPR analysis results, it was suggested that Mn, Cu, and Fe are the major contributors to the greyish-purple color. This study provides a reference for the specific experimental methods to determine chromophores and the origin of color in tremolite jades.