bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel Fluorinated Poly (Lactic-Co-Glycolic acid) (PLGA) and Polyethylene Glycol (PEG) Nanoparticles for Monitoring and Imaging in Osteoarthritis
Authors: Zerrillo Luana , Gupta Karthick Babu Sai Sankar , Lefeber Fons A.W.M. , Da Silva Candido G. , Galli Federica , Chan Alan , Veltien Andor , Dou Weiqiang , Censi Roberta , Di Martino Piera , Srinivas Mangala , Cruz Luis
Published: February 2021

Polymeric nanoparticles (NPs) find many uses in nanomedicine, from drug delivery to imaging. In this regard, poly (lactic-co-glycolic acid) (PLGA) and polyethylene glycol (PEG) particles are the most widely applied types of nano-systems due to their biocompatibility and biodegradability. Here we developed novel fluorinated polymeric NPs as vectors for multi-modal nanoprobes. This approach involved modifying polymeric NPs with trifluoroacetamide (TFA) and loading them with a near-infrared (NIR) dye for different imaging modalities, such as magnetic resonance imaging (MRI) and optical imaging. The PLGA-PEG-TFA NPs generated were characterized in vitro using the C28/I2 human chondrocyte cell line and in vivo in a mouse model of osteoarthritis (OA). The NPs were well absorbed, as confirmed by confocal microscopy, and were non-toxic to cells. To test the NPs as a drug delivery system for contrast agents of OA, the nanomaterial was administered via the intra-articular (IA) administration method. The dye-loaded NPs were injected in the knee joint and then visualized and tracked in vivo by fluorine-19 nuclear magnetic resonance and fluorescence imaging. Here, we describe the development of novel intrinsically fluorinated polymeric NPs modality that can be used in various molecular imaging techniques to visualize and track OA treatments and their potential use in clinical trials.
Antibiotic-Loaded Psyllium Husk Hemicellulose and Gelatin-Based Polymeric Films for Wound Dressing Application
Authors: Ahmad Naveed , Ahmad Muhammad Masood , Alruwaili Nabil K. , Alrowaili Ziyad Awadh , Alomar Fadhel Ahmed , Akhtar Sultan , Alsaidan Omar Awad , Alhakamy Nabil A. , Zafar Ameeduzzafar , Elmowafy Mohammed , Elkomy Mohammed H.
Published: February 2021

Wound infections are one of the major reasons for the delay in the healing of chronic wounds and can be overcome by developing effective wound dressings capable of absorbing exudate, providing local antibiotic release, and improving patient comfort. Arabinoxylan (AX) is a major hemicellulose present in psyllium seed husk (PSH) and exhibits promising characteristics for developing film dressings. Herein, AX-gelatin (GL) films were prepared by blending AX, gelatin (GL), glycerol, and gentamicin (antibiotic). Initially, the optimal quantities of AX, GL, and glycerol for preparing transparent, bubble-free, smooth, and foldable AX-GL films were found. Physiochemical, thermal, morphological, drug release, and antibacterial characteristics of the AX-GL films were evaluated to investigate their suitability as wound dressings. The findings suggested that the mechanical, water vapor transmission, morphological, and expansion characteristics of the optimized AX-GL films were within the required range for wound dressing. The results of Fourier-transform infrared (FTIR) analyses suggested chemical compatibility among the ingredients of the films. In in vitro drug release and antibacterial activity experiments, gentamicin (GM)-loaded AX-GL films released approximately 89% of the GM in 24 h and exhibited better antibacterial activity than standard GM solution. These results suggest that AX-GL films could serve as a promising dressing to protect against wound infections.
From Genetic Maps to QTL Cloning: An Overview for Durum Wheat
Authors: Colasuonno Pasqualina , Marcotuli Ilaria , Gadaleta Agata , Soriano Jose Miguel
Published: February 2021

Durum wheat is one of the most important cultivated cereal crops, providing nutrients to humans and domestic animals. Durum breeding programs prioritize the improvement of its main agronomic traits; however, the majority of these traits involve complex characteristics with a quantitative inheritance (quantitative trait loci, QTL). This can be solved with the use of genetic maps, new molecular markers, phenotyping data of segregating populations, and increased accessibility to sequences from next-generation sequencing (NGS) technologies. This allows for high-density genetic maps to be developed for localizing candidate loci within a few Kb in a complex genome, such as durum wheat. Here, we review the identified QTL, fine mapping, and cloning of QTL or candidate genes involved in the main traits regarding the quality and biotic and abiotic stresses of durum wheat. The current knowledge on the used molecular markers, sequence data, and how they changed the development of genetic maps and the characterization of QTL is summarized. A deeper understanding of the trait architecture useful in accelerating durum wheat breeding programs is envisioned.
Fine Characterization of a Resistance Phenotype by Analyzing TuYV-Myzus persicae-Rapeseed Interactions
Authors: Souquet Marlène , Pichon Elodie , Armand Thomas , Jacquot Emmanuel
Published: February 2021

Turnip yellows virus (TuYV), transmitted by Myzus persicae, can be controlled in rapeseed fields by insecticide treatments. However, the recent ban of the neonicotinoids together with the description of pyrethrinoid-resistant aphids has weakened insecticide-based control methods available to farmers. Since the deployment of insecticides in the 1980s, few research efforts were made to breed for rapeseed cultivars resistant to aphid-borne viral diseases. Thus, only few rapeseed cultivars released in Europe were reported to be TuYV-resistant, and the resistance phenotype of these cultivars was poorly characterized. In this study, several epidemiological parameters (infection rate, latency period, etc.) associated to the TuYV-resistance of the cv. Architect were estimated. Results showed a partial resistance phenotype for plants inoculated at the 2-/4-leaves stages and a resistance phenotype for plants inoculated at a more advanced growing stage. Moreover, analysis of infected plants highlighted (i) a poor quality of infected cv. Architect as a source of virus for transmission and (ii) an extended latency period for infected plants. Thus, dynamics of virus spread in the field should to be slower for Architect compared to susceptible rapeseed cultivars, which should lead to the maintenance of a higher proportion of healthy plants in the field.
Sampling Variation of RAD-Seq Data from Diploid and Tetraploid Potato (Solanum tuberosum L.)
Authors: Dang Zhenyu , Yang Jixuan , Wang Lin , Tao Qin , Zhang Fengjun , Zhang Yuxin , Luo Zewei
Published: February 2021

The new sequencing technology enables identification of genome-wide sequence-based variants at a population level and a competitively low cost. The sequence variant-based molecular markers have motivated enormous interest in population and quantitative genetic analyses. Generation of the sequence data involves a sophisticated experimental process embedded with rich non-biological variation. Statistically, the sequencing process indeed involves sampling DNA fragments from an individual sequence. Adequate knowledge of sampling variation of the sequence data generation is one of the key statistical properties for any downstream analysis of the data and for implementing statistically appropriate methods. This paper reports a thorough investigation on modeling the sampling variation of the sequence data from the optimized RAD-seq (Restriction sit associated DNA sequencing) experiments with two parents and their offspring of diploid and autotetraploid potato (Solanum tuberosum L.). The analysis shows significant dispersion in sampling variation of the sequence data over that expected under multinomial distribution as widely assumed in the literature and provides statistical methods for modeling the variation and calculating the model parameters, which may be easily implemented in real sequence datasets. The optimized design of RAD-seq experiments enabled effective control of presentation of undesirable chloroplast DNA and RNA genes in the sequence data generated.
Phenolic Acid Derivatives, Flavonoids and Other Bioactive Compounds from the Leaves of Cardiocrinum cordatum (Thunb.) Makino (Liliaceae)
Authors: Hori Kengo , Watanabe Takashi , Devkota Hari Prasad
Published: February 2021

Cardiocrinum cordatum (Thunb.) Makino (Family: Liliaceae), commonly known as ‘Ubayuri’, is native to Japan and some islands in the Russian Far East. It has high value as food, medicinal, and ornamental species. The aim of this study was to isolate and characterize the main chemical constituents of the leaves of C. cordatum. A total of 19 compounds, namely caffeic acid (1), caffeic acid methyl ester (2), caffeic acid β-glucopyranosyl ester (3), caffeic acid 4-O-β-glucopyranoside (4), ferulic acid (5), isoferulic acid (6), protocatechuic acid (7), syringic acid (8), 2,6-dimethoxy-p-hydroquinone 1-O-β-glucopyranoside (9), esculetin (10), taxifolin (11), quercetin 3-O-(6-O-α-rhamnopyranosyl)β-glucopyranoside-7-O-β-rhamnopyranoside (12), 2,7-dimethyl-2,4-diene-deca-α,ω-diacid β-glucopyranoside (13), 4-[formyl-5-(methoxymethyl)-1H-pyrrol-1-yl]butanoic acid (14), (3Z)-3-hexenyl β-glucopyranoside (15), tryptophan (16), adenine (17), adenosine (18), and 2-deoxyadenosine (19) were isolated using various chromatographic methods. The structures of isolated compounds were elucidated on the basis of their NMR spectroscopic data. All these compounds were isolated for the first time from the genus Cardiocrinum. Phenolic acid derivatives and flavonoids can be considered as chemotaxonomic markers in the leaves of Cardiocrinum species.
Cloning and Characterization of Three Sugar Metabolism Genes (LBGAE, LBGALA, and LBMS) Regulated in Response to Elevated CO2 in Goji Berry (Lycium barbarum L.)
Authors: Ma Yaping , Devi Mura Jyostna , R. Reddy Vangimalla , Song Lihua , Gao Handong , Cao Bing
Published: February 2021

The composition and content of sugar play a pivotal role in goji berry (Lycium barbarum L.) fruits, determining fruit quality. Long-term exposure of goji berry to elevated CO2 (eCO2) was frequently demonstrated to reduce sugar content and secondary metabolites. In order to understand the regulatory mechanisms and improve the quality of fruit in the changing climate, it is essential to characterize sugar metabolism genes that respond to eCO2. The objectives of this study were to clone full-length cDNA of three sugar metabolism genes—LBGAE (Lycium barbarum UDP-glucuronate 4-epimerase), LBGALA (Lycium barbarum alpha-galactosidase), and LBMS (Lycium barbarum malate synthase)—that were previously identified responding to eCO2, and to analyze sequence characteristics and expression regulation patterns. Sugar metabolism enzymes regulated by these genes were also estimated along with various carbohydrates from goji berry fruits grown under ambient (400 μmol mol−1) and elevated (700 μmol mol−1) CO2 for 90 and 120 days. Homology-based sequence analysis revealed that the protein-contained functional domains are similar to sugar transport regulation and had a high sequence homology with other Solanaceae species. The sucrose metabolism-related enzyme’s activity varied significantly from ambient to eCO2 in 90-day and 120-day samples along with sugars. This study provides fundamental information on sugar metabolism genes to eCO2 in goji berry to enhance fruit quality to climate change.
The CBL-Interacting Protein Kinase NtCIPK23 Positively Regulates Seed Germination and Early Seedling Development in Tobacco (Nicotiana tabacum L.)
Authors: Shi Sujuan , An Lulu , Mao Jingjing , Aluko Oluwaseun Olayemi , Ullah Zia , Xu Fangzheng , Liu Guanshan , Liu Haobao , Wang Qian
Published: February 2021

CBL-interacting protein kinase (CIPK) family is a unique group of serine/threonine protein kinase family identified in plants. Among this family, AtCIPK23 and its homologs in some plants are taken as a notable group for their importance in ions transport and stress responses. However, there are limited reports on their roles in seedling growth and development, especially in Solanaceae plants. In this study, NtCIPK23, a homolog of AtCIPK23 was cloned from Nicotiana tabacum. Expression analysis showed that NtCIPK23 is mainly expressed in the radicle, hypocotyl, and cotyledons of young tobacco seedlings. The transcriptional level of NtCIPK23 changes rapidly and spatiotemporally during seed germination and early seedling growth. To study the biological function of NtCIPK23 at these stages, the overexpressing and CRISPR/Cas9-mediated knock-out (ntcipk23) tobacco lines were generated. Phenotype analysis indicated that knock-out of NtCIPK23 significantly delays seed germination and the appearance of green cotyledon of young tobacco seedling. Overexpression of NtCIPK23 promotes cotyledon expansion and hypocotyl elongation of young tobacco seedlings. The expression of NtCIPK23 in hypocotyl is strongly upregulated by darkness and inhibited under light, suggesting that a regulatory mechanism of light might underlie. Consistently, a more obvious difference in hypocotyl length among different tobacco materials was observed in the dark, compared to that under the light, indicating that the upregulation of NtCIPK23 contributes greatly to the hypocotyl elongation. Taken together, NtCIPK23 not only enhances tobacco seed germination, but also accelerate early seedling growth by promoting cotyledon greening rate, cotyledon expansion and hypocotyl elongation of young tobacco seedlings.
Phytosanitary Interventions for Safe Global Germplasm Exchange and the Prevention of Transboundary Pest Spread: The Role of CGIAR Germplasm Health Units
Authors: Kumar P. Lava , Cuervo Maritza , Kreuze J. F. , Muller Giovanna , Kulkarni Gururaj , Kumari Safaa G. , Massart Sebastien , Mezzalama Monica , Alakonya Amos , Muchugi Alice , Graziosi Ignazio , Ndjiondjop Marie-Noelle , Sharma Rajan , Negawo Alemayehu Teressa
Published: February 2021

The inherent ability of seeds (orthodox, intermediate, and recalcitrant seeds and vegetative propagules) to serve as carriers of pests and pathogens (hereafter referred to as pests) and the risk of transboundary spread along with the seed movement present a high-risk factor for international germplasm distribution activities. Quarantine and phytosanitary procedures have been established by many countries around the world to minimize seed-borne pest spread by screening export and import consignments of germplasm. The effectiveness of these time-consuming and cost-intensive procedures depends on the knowledge of pest distribution, availability of diagnostic tools for seed health testing, qualified operators, procedures for inspection, and seed phytosanitation. This review describes a unique multidisciplinary approach used by the CGIAR Germplasm Health Units (GHUs) in ensuring phytosanitary protection for the safe conservation and global movement of germplasm from the 11 CGIAR genebanks and breeding programs that acquire and distribute germplasm to and from all parts of the world for agricultural research and food security. We also present the challenges, lessons learned, and recommendations stemming from the experience of GHUs, which collaborate with the national quarantine systems to export and distribute about 100,000 germplasm samples annually to partners located in about 90 to 100 countries. Furthermore, we describe how GHUs adjust their procedures to stay in alignment with evolving phytosanitary regulations and pest risk scenarios. In conclusion, we state the benefits of globally coordinated phytosanitary networks for the prevention of the intercontinental spread of pests that are transmissible through plant propagation materials.
Ecological Status of Coralligenous Macroalgal Assemblages in the Marine Protected Area (MPA) Isole Ciclopi (Ionian Sea)
Authors: Costanzo Luca Giuseppe , Marletta Giuliana , Alongi Giuseppina
Published: February 2021

The coralligenous habitat represents one of the most important hotspots of Mediterranean biodiversity. However, along the Ionian coast of Sicily (Italy) the coralligenous macroalgal assemblages have always been poorly studied. The present study was carried out in the coralligenous habitat of the Marine Protected Area (MPA) Isole Ciclopi, located along the central-eastern coast of Sicily. Previously, only a few floristic studies, including some data on the coralligenous flora, were conducted within this MPA in the 1970s and 2001. Therefore, the present study aimed to gain an updated knowledge on the coralligenous flora and to compare the current data with data derived from the previous floristic studies, to observe if in the last 50 years environmental changes occurred and to monitor the effectiveness of the MPA in protecting this habitat. In particular, the coralligenous flora of the MPA was analyzed through remotely operated vehicles (ROV) surveys and destructive samples. ROV surveys allow us to observe that the coralligenous assemblages of the MPA are well-structured, especially regarding the encrusting Rhodophyta, which showed the highest percent cover among the main morphological groups/taxa. Through the sample analysis in the laboratory, a total of 92 taxa has been recorded. Comparing the floristic lists of the present research and the past studies, an increase of local biodiversity was highlighted. Nevertheless, an increment of Non-Indigenous Species (NIS), warm-water species, and Rhodophyta with wide ecological valence was also noted. The main causes of these variations in the coralligenous flora have been traced back to reduced water transparency, maybe due to sedimentation, and a rise in the seawater temperature. Therefore, although the coralligenous assemblages of the MPA Isole Ciclopi appear to be well-structured, future studies will be necessary to continue monitoring this habitat to evaluate whether the MPA is effective in safeguarding this hotspot of biodiversity.
Tomato Bio-Protection Induced by Pseudomonas fluorescens N21.4 Involves ROS Scavenging Enzymes and PRs, without Compromising Plant Growth
Authors: García-Villaraco Ana , Boukerma Lamia , Lucas Jose Antonio , Gutierrez-Mañero Francisco Javier , Ramos-Solano Beatriz
Published: February 2021

Aims: to discover the interrelationship between growth, protection and photosynthesis induced by Pseudomonas fluorescens N21.4 in tomato (Lycopersicum sculentum) challenged with the leaf pathogen Xanthomonas campestris, and to define its priming fingerprint. Methods: Photosynthesis was determined by fluorescence; plant protection was evaluated by relative disease incidence, enzyme activities by specific colorimetric assays and gene expression by qPCR. Changes in Reactive Oxygen Species (ROS) scavenging cycle enzymes and pathogenesis related protein activity and expression were determined as metabolic and genetic markers of induction of systemic resistance. Results: N21.4 significantly protected plants and increased dry weight. Growth increase is supported by significant increases in photochemical quenching together with significant decreases in energy dissipation (Non-Photochemical Quenching, NPQ). Protection was associated with changes in ROS scavenging cycle enzymes, which were significantly increased on N21.4 + pathogen challenged plants, supporting the priming effect. Superoxide Dismutase (SOD) was a good indicator of biotic stress, showing similar levels in pathogen- and N21.4-treated plants. Similarly, the activity of defense-related enzymes, ß-1,3-glucanase and chitinase significantly increased in post-pathogen challenge state; changes in gene expression were not coupled to activity. Conclusions: protection does not compromise plant growth; N21.4 priming fingerprint is defined by enhanced photochemical quenching and decreased energy dissipation, enhanced chlorophylls, primed ROS scavenging cycle enzyme activity, and glucanase and chitinase activity.
Anti-Inflammatory Effect of Artemisia argyi on Ethanol-Induced Gastric Ulcer: Analytical, In Vitro and In Vivo Studies for the Identification of Action Mechanism and Active Compounds
Authors: Shin Myoung-Sook , Lee Jaemin , Lee Jin Woo , Park Se Hoon , Lee Il Kyun , Choi Jung A. , Lee Jung Suk , Kang Ki Sung
Published: February 2021

Artemisia argyi is widely used as traditional medicine in East Asia. However, its effects against inflammation and gastric ulcers have not been reported yet. We analyzed anti-inflammatory activity and its molecular mechanisms of A. argyi using RAW264.7 cells line, then evaluated the curative efficacy in rats with acute gastric ulcers. Nitric oxide and IL-6 production was measured using Griess reagent and an ELISA kit. Inducible nitric oxide synthase (iNOS), interleukin (IL)-6, and mucin (MUC)1, MUC5AC, and MUC6 mRNA were determined by SYBR Green or Taqman qRT-PCR methods. The phosphorylation of ERK, JNK, p38, and c-Jun protein were detected by western blotting. RW0117 inhibited LPS-induced NO and IL-6 production. The mRNA levels of iNOS and IL-6 were strongly suppressed. The phosphorylation of ERK, JNK, and c-Jun decreased by treatment with RW0117. Oral administration of RW0117 recovered the amount of mucin mRNA and protein level that was decreased due to gastric ulcers by HCl-EtOH. A. argyi exhibited strong anti-inflammatory effects and contributed to the modulation of HCl-EtOH-induced gastric ulcer in rats.
Ethephon Activates the Transcription of Senescence-Associated Genes and Nitrogen Mobilization in Grapevine Leaves (Vitis vinifera cv. Riesling)
Authors: Hendgen Maximilian , Günther Stefan , Schubert Sven , Löhnertz Otmar
Published: February 2021

Nitrogen (N) remobilization in the context of leaf senescence is of considerable importance for the viability of perennial plants. In late-ripening crops, such as Vitis vinifera, it may also affect berry ripening and fruit quality. Numerous studies on the model plant Arabidopsis thaliana have confirmed an involvement of the plant hormone ethylene in the regulation of senescence. However, ethylene research on grapevine was mostly focused on its involvement in berry ripening and stress tolerance until now. To investigate the effect of ethylene on the initiation, regulation, and progress of senescence-dependent N mobilization in grapevine leaves, we treated field-grown Vitis vinifera cv. Riesling vines with 25 mM ethephon at the end of berry ripening. Ethephon induced premature chlorophyll degradation and caused a shift of the leaf transcriptome equivalent to developmental leaf senescence. The upregulated metabolic processes covered the entire N remobilization process chain, altered the amino acid composition in the leaves, and resulted in an average 60% decrease in leaf N. Our findings increase the fundamental knowledge about the initiation and manipulation of leaf N remobilization in perennial woody plants by ethephon. This offers a methodological approach to the targeted induction of senescence and thus to an improvement in the N supply of grapes.
Synthesis and Thermo-Mechanical Study of Epoxy Resin-Based Composites with Waste Fibers of Hemp as an Eco-Friendly Filler
Authors: Gargol Mateusz , Klepka Tomasz , Klapiszewski Łukasz , Podkościelna Beata
Published: February 2021

The synthesis, thermal, and mechanical properties of epoxy resin composites incorporating waste fibers of hemp were studied. Five different systems with increasing quantity of the eco-filler were obtained. For the synthesis of polymeric materials, the commercial epoxy resins Epidian® 5 and triethylenetetramine (TETA) were applied as crosslinking agents. The composites were obtained based on the polyaddition reaction of an amine group with an epoxide ring. ATR/FT-IR (Attenuated Total Reflection-Fourier Transform Infrared) analysis was used to confirm the chemical structure of the composites and the course of curing processes. Moreover, the influence of the eco-friendly components on the mechanical properties was determined, while thermal properties of the materials were investigated by thermogravimetry analysis (TGA) and differential scanning calorimetry (DSC). Dynamic mechanical studies (DMA) and Shore hardness tests of the obtained polymers were also carried out. The DSC curves and DMA analysis revealed that all materials were characterized by a similar glass transition range. Furthermore, the DMA and hardness measurements of the composites demonstrated an increasing elasticity with the increase in the amount of eco-filler present in the compositions.
Evaluating Antibacterial Efficacy and Biocompatibility of PAN Nanofibers Loaded with Diclofenac Sodium Salt
Authors: Sarwar Muhammad Nauman , Ullah Azeem , Haider Md. Kaiser , Hussain Nadir , Ullah Sana , Hashmi Motahira , Khan Muhammad Qamar , Kim Ick Soo
Published: February 2021

Side effects of the drugs’ oral administration led us to examine the possibility of using diclofenac sodium (DLF) in a polymeric drug delivery system based on electrospun polyacrylonitrile (PAN) nanofibers, which can be produced cost-effectively and with good applicability for transdermal treatments. The inclusion of DLF in PAN nanofibers increased the nanofiber diameter from ~200 nm to ~500 nm. This increase can be attributed to the increase in the spinning solution viscosity. FTIR spectra confirm the entrapment of the DLF into the PAN nanofibers. X-ray diffraction pattern showed that the inclusion of the DLF in the PAN nanofibers had caused the misalignment in the polymeric chains of the PAN, thus resulting in a decrease of the peak intensity at 2θ = 17o. The DLF loaded PAN nanofibers were efficient against the gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli), with maximum inhibition zone of 16 ± 0.46 mm for E. coli and 15.5 ± 0.28 mm for S. aureus. Good cell viability ~95% for L929 cells in more extended incubation periods was reported. A gradual release of DLF from the PAN nanofiber was observed and can be attributed to the stability of Pan in PBS medium. Cell adhesion micrographs show that cell-cell interaction is stronger than the cell-material interaction. This type of weak cell interaction with the wound dressing is particularly advantageous, as this will not disturb the wound surface during the nursing of the wound.
Magnetic Polymer Composite Particles: Design and Magnetorheology
Authors: Lu Qi , Choi Kisuk , Nam Jae-Do , Choi Hyoung Jin
Published: February 2021

As a family of smart functional hybrid materials, magnetic polymer composite particles have attracted considerable attention owing to their outstanding magnetism, dispersion stability, and fine biocompatibility. This review covers their magnetorheological properties, namely, flow curve, yield stress, and viscoelastic behavior, along with their synthesis. Preparation methods and characteristics of different types of magnetic composite particles are presented. Apart from the research progress in magnetic polymer composite synthesis, we also discuss prospects of this promising research field.
Fast Curable Polysiloxane-Silphenylene Hybrimer with High Transparency and Refractive Index for Optical Applications
Authors: Koh Kyungkuk , Sohn Honglae
Published: February 2021

In this study, a fast curable polysiloxane-silphenylene hybrimer (PSH) was synthesized by the nonhydrolytic sol–gel condensation of phenyl-vinyl-oligosiloxane (PVO) and tris(dimethylhydrosilyl)benzene (TDMSB) under a Pt catalyst to investigate its optical property and thermal stability. The combination of PVO and tripod-type TDMSB results in a hybrimer with a fast curing time of 30 min. The PSH exhibited a high refractive index of 1.60, 1.59, and 1.58 at 450, 520, and 635 nm, respectively. High transmittance of 97% at 450 nm was obtained. The PSH exhibited a very high transmittance of 97% before thermal aging. The high optical transmittance of the PSH was only slightly decreased by 0.5% of the transmittance at 180 °C for 72 h after thermal aging, and high transparency was maintained almost constant even after 72 h of high-temperature treatment.
Evaluation of Polyacrylonitrile Nonwoven Mats and Silver–Gold Bimetallic Nanoparticle-Decorated Nonwoven Mats for Potential Promotion of Wound Healing In Vitro and In Vivo and Bone Growth In Vitro
Authors: Bai Meng-Yi , Ku Fang-Yu , Shyu Jia-Fwu , Hayashi Tomohiro , Wu Chia-Chun
Published: February 2021

We prepared polyacrylonitrile (PAN) and urchin-like Ag–Au bimetallic or Ag nanoparticle-decorated PAN nonwoven mats using electrospinning and evaluated them in vitro and in vivo for wound healing, antibacterial effects on skin tissue, and promotion of bone ingrowth in vitro. A facile, green, low-temperature protocol was developed to obtain these nonwoven mats. The sterilization rate of urchin-like Ag–Au bimetallic and Ag nanoparticle-decorated PAN nonwoven mats against Staphylococcus aureus was 96.81 ± 2.81% and 51.90 ± 9.07%, respectively, after 5 h treatment. In an in vitro cell model, these two mats did not show significant toxicity; cell viability of >80% was obtained within 5 h of treatment. In vivo animal model preclinical assessment showed that the urchin-like Ag–Au bimetallic nonwoven mat group showed significant wound recovery because of sebaceous gland, hair follicle, and fat formation during skin tissue regeneration; increased neovascularization and compact collagen fibers were observed in the dermal layer, comparable to the findings for the control group. The mother substrate of the urchin-like Ag–Au bimetallic nanoparticle-decorated PAN nonwoven mats, that is, pure PAN nonwoven mats, was found to be a potential scaffold for bone tissue engineering as osteoblast ingrowth from the top to the bottom of the membrane and proliferation inside the membrane were observed. The key genetic factor Cbfa1 was identified as a key osteoblast differentiation regulator in vitro. Thus, electrospun membrane materials show potential for use as dual-functional biomaterials for bone regeneration and infection control and composite grafts for infectious bone and soft tissue defects.
Analysis of the Impact on the Mechanical Properties of Modification of Oligohydroxyethers in Organic Solvent Solution with Rubbers
Authors: Bezgin Vitalii , Dudek Agata , Gnatowski Adam
Published: February 2021

This paper proposes and presents the chemical modification of linear hydroxyethers (LHE) with different molecular weights (380, 640, and 1830 g/mol) with the addition of three types of rubbers (polysulfide rubber (PSR), polychloroprene rubber (PCR), and styrene-butadiene rubber (SBR)). The main purpose of choosing this type of modification and the materials used was the possibility to use it in industrial settings. The modification process was conducted for a very wide range of modifier additions (rubber) per 100 g LHE. The materials obtained in the study were subjected to strength tests in order to determine the effect of the modification on functional properties. Mechanical properties of the modified materials were improved after the application of the modifier (rubber) to polyhydroxyether (up to certain modifier content). The most favorable changes in the tested materials were registered in the modification of LHE-1830 with PSR. In the case of LHE-380 and LHE-640 modified in cyclohexanol (CH) and chloroform (CF) solutions, an increase in the values of the tested properties was also obtained, but to a lesser extent than for LHE-1830. The largest changes were registered for LHE-1830 with PSR in CH solution: from 12.1 to 15.3 MPa for compressive strength tests, from 0.8 to 1.5 MPa for tensile testing, from 0.8 to 14.7 MPa for shear strength, and from 1% to 6.5% for the maximum elongation. The analysis of the available literature showed that the modification proposed by the authors has not yet been presented in any previous scientific paper.
One-Shot Fabrication of Polymeric Hollow Microneedles by Standard Photolithography
Authors: Dardano Principia , De Martino Selene , Battisti Mario , Miranda Bruno , Rea Ilaria , De Stefano Luca
Published: February 2021

Microneedles (MNs) are an emerging technology in pharmaceutics and biomedicine, and are ready to be commercialized in the world market. However, solid microneedles only allow small doses and time-limited administration rates. Moreover, some well-known and already approved drugs need to be re-formulated when supplied by MNs. Instead, hollow microneedles (HMNs) allow for rapid, painless self-administrable microinjection of drugs in their standard formulation. Furthermore, body fluids can be easily extracted for analysis by a reverse use of HMNs, thus making them perfect for sensing issues and theranostics applications. The fabrication of HMNs usually requires several many-step processes, increasing the costs and consequently decreasing the commercial interest. Photolithography is a well-known fabrication technique in microelectronics and microfluidics that fabricates MNs. In this paper, authors show a proof of concept of a patented, easy and one-shot fabrication of two kinds of HMNs: (1) Symmetric HMNs with a “volcano” shape, made by using a photolithographic mask with an array of transparent symmetric rings; and (2) asymmetric HMNs with an oblique aperture, like standard hypodermic steel needles, made by using an array of transparent asymmetric rings, defined by two circles, which centers are slightly mismatched. Simulation of light propagation, fabrication process, and preliminary results on ink microinjection are presented.
Synthesis and Antibacterial Activity of Cationic Amino Acid-Conjugated Dendrimers Loaded with a Mixture of Two Triterpenoid Acids
Authors: Schito Anna Maria , Schito Gian Carlo , Alfei Silvana
Published: February 2021

To counteract the growing bacterial resistance, we previously reported the remarkable antimicrobial activity of amino acid-conjugated cationic dendrimers (CDs) against several Gram-negative species, establishing that the cationic lysine was essential for their potency. In this paper, CDs conjugated with lysine and arginine and encapsulating ursolic and oleanolic acids (UOACDs) were assumed to be excellent candidates for developing new antibacterial agents, possibly active against Gram-positive species. Indeed, both the guanidine group of arginine and the two triterpenoid acids are items known for directing antibacterial effects, particularly against Gram-positive bacteria. The cationic dendrimers were obtained by peripheral conjugation with the selected amino acids and by entrapping a physical mixture of the commercial triterpenoid acids. The cationic compounds were characterized and successfully tested against 15 Gram-positive isolates. Interesting minimum inhibitory concentration (MIC) values were obtained for all the dendrimer-drug agents, establishing that the antibacterial activity observed for the UOACDs strongly depended on the density and on the type of the cationic groups of the cationic amino acid-conjugated dendrimers and not on the presence and the release of UOA. Particularly, lysine was critical for potency, while arginine was critical for redirecting activity against Gram-positive species. Especially, a high cationic character, associated with a balanced content of lysine/arginine, produced a remarkable antimicrobial effect (MIC = 0.5–8.7 µM).
Digital Media: When God Becomes Everybody—The Blurring of Sacred and Profane
Authors: Tsuria Ruth
Published: February 2021

This article explores the relationship between communication technology and religion. While previous research has focused on how religious institutions and individuals use digital media, this article emphasizes the religious feelings digital media seem to invoke, with examples like the Jesus Phone or Kopimism. This is explained using theories from Religious Studies. Borrowing from Durkheim, digital media are examined as “sacred” and as “profane”. It is suggested that digital media can be both sacred and profane because hypermodern societies have sanctified the profane. More specifically, hypermodern societies have “killed” god and replaced it with the human, with everybody. It is then digital media—a tool that is meant to be owned by everybody and represent everybody—that take the place of the divine. This tool then, because it connects and communicates human needs and everyday thoughts (and not despite that), inspires feelings of awe and sanctity, even as we use it for the most profane activities.
Performance and Feasibility of Drone-Mounted Imaging Spectroscopy for Invasive Aquatic Vegetation Detection
Authors: Bolch Erik A. , Hestir Erin L. , Khanna Shruti
Published: February 2021

Invasive plants are non-native species that can spread rapidly, leading to detrimental economic, ecological, or environmental impact. In aquatic systems such as the Sacramento-San Joaquin River Delta in California, USA, management agencies use manned aerial vehicles (MAV) imaging spectroscopy missions to map and track annual changes in invasive aquatic plants. Advances in unmanned aerial vehicles (UAV) and sensor miniaturization are enabling higher spatial resolution species mapping, which is promising for early detection of invasions before they spread over larger areas. This study compared maps made from UAV-based imaging spectroscopy with the manned airborne imaging spectroscopy-derived maps that are currently produced for monitoring invasive aquatic plants in the Sacramento-San Joaquin Delta. Concurrent imagery was collected using the MAV mounted HyMap sensor and the UAV mounted Nano-Hyperspec at a wetland study site and classification maps generated using random forest models were compared. Classification accuracies were comparable between the Nano- and HyMap-derived maps, with the Nano-derived map having a slightly higher overall accuracy. Additionally, the higher resolution of the Nano imagery allowed detection of patches of water hyacinth present in the study site that the HyMap could not. However, it would not be feasible to operate the Nano as a replacement to HyMap at scale despite its improved detection capabilities due to the high costs associated with overcoming area coverage limitations. Overall, UAV-based imaging spectroscopy provides comparable or improved capability, and we suggest it could be used to supplement existing monitoring programs by focusing on target areas of high ecologic or economic priority.
Machine Learning Classification of Mediterranean Forest Habitats in Google Earth Engine Based on Seasonal Sentinel-2 Time-Series and Input Image Composition Optimisation
Authors: Praticò Salvatore , Solano Francesco , Di Fazio Salvatore , Modica Giuseppe
Published: February 2021

The sustainable management of natural heritage is presently considered a global strategic issue. Owing to the ever-growing availability of free data and software, remote sensing (RS) techniques have been primarily used to map, analyse, and monitor natural resources for conservation purposes. The need to adopt multi-scale and multi-temporal approaches to detect different phenological aspects of different vegetation types and species has also emerged. The time-series composite image approach allows for capturing much of the spectral variability, but presents some criticalities (e.g., time-consuming research, downloading data, and the required storage space). To overcome these issues, the Google Earth engine (GEE) has been proposed, a free cloud-based computational platform that allows users to access and process remotely sensed data at petabyte scales. The application was tested in a natural protected area in Calabria (South Italy), which is particularly representative of the Mediterranean mountain forest environment. In the research, random forest (RF), support vector machine (SVM), and classification and regression tree (CART) algorithms were used to perform supervised pixel-based classification based on the use of Sentinel-2 images. A process to select the best input image (seasonal composition strategies, statistical operators, band composition, and derived vegetation indices (VIs) information) for classification was implemented. A set of accuracy indicators, including overall accuracy (OA) and multi-class F-score (Fm), were computed to assess the results of the different classifications. GEE proved to be a reliable and powerful tool for the classification process. The best results (OA = 0.88 and Fm = 0.88) were achieved using RF with the summer image composite, adding three VIs (NDVI, EVI, and NBR) to the Sentinel-2 bands. SVM and RF produced OAs of 0.83 and 0.80, respectively.
Improving Soil Moisture Estimation by Identification of NDVI Thresholds Optimization: An Application to the Chinese Loess Plateau
Authors: Yuan Lina , Li Long , Zhang Ting , Chen Longqian , Zhao Jianlin , Liu Weiqiang , Cheng Liang , Hu Sai , Yang Longhua , Wen Mingxin
Published: February 2021

Accuracy soil moisture estimation at a relevant spatiotemporal scale is scarce but beneficial for understanding ecohydrological processes and improving weather forecasting and climate models, particularly in arid and semi-arid regions like the Chinese Loess Plateau (CLP). This study proposed Criterion 2, a new method to improve relative soil moisture (RSM) estimation by identification of normalized difference vegetation index (NDVI) thresholds optimization based on our previously proposed iteration procedure of Criterion 1. Apparent thermal inertia (ATI) and temperature vegetation dryness index (TVDI) were applied to subregional RSM retrieval for the CLP throughout 2017. Three optimal NDVI thresholds (NDVI0 was used for computing TVDI, and both NDVIATI and NDVITVDI for dividing the entire CLP) were firstly identified with the best validation results (R¯) of subregions for 8-day periods. Then, we compared the selected optimal NDVI thresholds and estimated RSM with each criterion. Results show that NDVI thresholds were optimized to robust RSM estimation with Criterion 2, which characterized RSM variability better. The estimated RSM with Criterion 2 showed increased accuracy (maximum R¯ of 0.82 ± 0.007 for Criterion 2 and of 0.75 ± 0.008 for Criterion 1) and spatiotemporal coverage (45 and 38 periods (8-day) of RSM maps and the total RSM area of 939.52 × 104 km2 and 667.44 × 104 km2 with Criterion 2 and Criterion 1, respectively) than with Criterion 1. Moreover, the additional NDVI thresholds we applied was another strategy to acquire wider coverage of RSM estimation. The improved RSM estimation with Criterion 2 could provide a basis for forecasting drought and precision irrigation management.
Hydrological and Kinematic Precursors of the 2017 Calving Event at the Petermann Glacier in Greenland Observed from Multi-Source Remote Sensing Data
Authors: Li Daan , Jiang Liming , Huang Ronggang
Published: February 2021

Both a decrease of sea ice and an increase of surface meltwater, which may induce ice-flow speedup and frontal collapse, have a significant impact on the stability of the floating ice shelf in Greenland. However, detailed dynamic precursors and drivers prior to a fast-calving process remain unclear due to sparse remote sensing observations. Here, we present a comprehensive investigation on hydrological and kinematic precursors before the calving event on 26 July 2017 of Petermann Glacier in northern Greenland, by jointly using remote sensing observations at high-temporal resolution and an ice-flow model. Time series of ice-flow velocity fields during July 2017 were retrieved with Sentinel-2 observations with a sub-weekly sampling interval. The ice-flow speed quickly reached 30 m/d on 26 July (the day before the calving), which is roughly 10 times quicker than the mean glacier velocity. Additionally, a significant decrease in the radar backscatter coefficient of Sentinel-1 images suggests a rapid transformation from landfast sea ice into open water, associated with a decrease in sea ice extent. Additionally, the area of melt ponds on the floating ice tongue began to increase in mid-May, quickly reached a peak at the end of June and lasted for nearly one month until the calving occurred. We used the ice sheet system model to model the spatial-temporal damage and stress on the floating ice, thereby finding an abnormal stress distribution in a cracked region. It is inferred that this calving event may relate to a weakening of the sea ice, shearing of the tributary glacier, and meltwater infiltrating crevasses.
Fast Implementation of Insect Multi-Target Detection Based on Multimodal Optimization
Authors: Wang Rui , Zhang Yiming , Tian Weiming , Cai Jiong , Hu Cheng , Zhang Tianran
Published: February 2021

Entomological radars are important for scientific research of insect migration and early warning of migratory pests. However, insects are hard to detect because of their tiny size and highly maneuvering trajectory. Generalized Radon–Fourier transform (GRFT) has been proposed for effective weak maneuvering target detection by long-time coherent detection via jointly motion parameter search, but the heavy computational burden makes it impractical in real signal processing. Particle swarm optimization (PSO) has been used to achieve GRFT detection by fast heuristic parameter search, but it suffers from obvious detection probability loss and is only suitable for single target detection. In this paper, we convert the realization of GRFT into a multimodal optimization problem for insect multi-target detection. A novel niching method without radius parameter is proposed to detect unevenly distributed insect targets. Species reset and boundary constraint strategy are used to improve the detection performance. Simulation analyses of detection performance and computational cost are given to prove the effectiveness of the proposed method. Furthermore, real observation data acquired from a Ku-band entomological radar is used to test this method. The results show that it has better performance on detected target amount and track continuity in insect multi-target detection.
Multi-Source EO for Dynamic Wetland Mapping and Monitoring in the Great Lakes Basin
Authors: Battaglia Michael J. , Banks Sarah , Behnamian Amir , Bourgeau-Chavez Laura , Brisco Brian , Corcoran Jennifer , Chen Zhaohua , Huberty Brian , Klassen James , Knight Joseph , Morin Paul , Murnaghan Kevin , Pelletier Keith , White Lori
Published: February 2021

Wetland managers, citizens and government leaders are observing rapid changes in coastal wetlands and associated habitats around the Great Lakes Basin due to human activity and climate variability. SAR and optical satellite sensors offer cost effective management tools that can be used to monitor wetlands over time, covering large areas like the Great Lakes and providing information to those making management and policy decisions. In this paper we describe ongoing efforts to monitor dynamic changes in wetland vegetation, surface water extent, and water level change. Included are assessments of simulated Radarsat Constellation Mission data to determine feasibility of continued monitoring into the future. Results show that integration of data from multiple sensors is most effective for monitoring coastal wetlands in the Great Lakes region. While products developed using methods described in this article provide valuable management tools, more effort is needed to reach the goal of establishing a dynamic, near-real-time, remote sensing-based monitoring program for the basin.