bluuebunny/crossref_metadata_2025 huggingface dataset:
Etika Bisnis Islam pada Pedagang Kuliner di Putussibau Kalimantan Barat
Authors: Jumratul Hasanah  , Sri Deti  , Beti Yanuri Posha
Published: February 2024

Abstract : This research uses a qualitative approach with the type of field research. The subjects of this research were culinary traders in Alun Putussibau park, namely food and drink traders. Data sources consist of primary data and secondary data with data collection techniques using interviews, observation and documentation. The research results show that 1). Culinary traders in Taman Alun Putussibau are able to implement 3 aspects of the Islamic business axioms, namely balance, free will and responsibility, but have not been able to implement the unity aspect. 2). The obstacle faced by culinary traders in implementing Islamic business ethics is that they prioritize trading rather than carrying out their obligations as a Muslim. Apart from that, there are obstacles in terms of poor waste management regulations, so it is hoped that there will be good cooperation between culinary traders, buyers and the relevant government. 3). The implementation of Islamic business ethics for culinary traders in Taman Alun Putussibau is to increase education about the importance of trading based on Islamic business ethics so that culinary traders can communicate well and in accordance with Islamic law.
Abstrak : Penelitian ini menggunakan pendekatan kualitatif dengan jenis penelitian Lapangan (Field Research). Subjek penelitian ini adalah para pedagang kuliner di taman Alun Putussibau yaitu para pedagang makanan dan minuman. Sumber data terdiri dari data primer dan data sekunder dengan teknik pengumpulan data menggunakan wawancara, observasi dan dokumentasi. Hasil penelitian menunjukan bahwa 1). Pedagang kuliner di Taman Alun Putussibau mampu melaksanakan 3 aspek dari aksioma bisnis Islam yaitu keseimbangan (equilibrium), kehendak bebas (free will) dan tanggungjawab (responsibility) namun belum mampu mengimplementasikan aspek kesatuan (unity). 2). Kendala yang dihadapi pedagang kuliner dalam implementasi etika bisnis Islam yaitu pemikiran lebih mementingkan berdagang daripada melaksanakan  kewajiban sebagai  seorang muslim.  Selain  itu  terdapat  kendala dalam regulasi pengelolaan sampah yang kurang baik sehingga diharapkan adanya kejasama yang baik antar pedagang kuliner, Pembeli maupun Pemerintah terkait. 3). Implementasi etika bisnis Islam pada pedagang kuliner di Taman Alun Putussibau adalah menambah edukasi tentang pentingnya berdagang yang berlandaskan etika bisnis Islam dengan demikian para pedagang kuliner dapat bermuamalah dengan baik dan sesuai dengan syariat Islam.
PERLINDUNGAN HUKUM HAK DESAIN INDUSTRI TERKAIT DENGAN KEMASAN PRODUK JAHE (Studi Putusan No. 583K/Pdt.SUS-HKI/2021)
Authors: M. Aulia Zikry Lubis  , Saidin  , Rosnidar Sembiring  , Jelly Leviza
Published: February 2024

Pelaksanaan hak desain industri di tengah masyarakat ada beberapa hal, hak desain industri telah nyata belum mampu untuk melindungi kepentingan pemegang hak eksklusifnya, hal tersebut disebabkan karena beberapa faktor, diantaranya adalah adanya konflik kepentingan politik pemegang kekuasaan. Permasalahan dalam penelitian kepastian hukum dari pemilik desain industri terdaftar atas adanya kemiripan disain industri yang memiliki kesamaan. Akibat hukum setelah adanya putusan kasasi oleh Mahkamah Agung terhadap sengketa unsur kebaruan desain industri kemasan produk jahe. Bentuk perlindungan hukum pada pemegang hak desain industri terkait dengan kemasan produk jahe dalam Putusan 583K/Pdt.SUS-HKI/2021. Sifat penelitian adalah deskriptif. jenis penelitian penelitian hukum normative. Sumber data yang digunakan data sekunder. Pengumpulan data dilakukan dengan cara penelitian kepustakaan. Analisis data yang digunakan metode kualitatif. Kepastian hukum dari pemilik desain industri terdaftar atas adanya kemiripan desain industri yang memiliki kesamaan. Kepastian hukum dapat dicapai melalui putusan pengadilan. Prinsip kepastian hukum mengacu pada keyakinan bahwa hukum harus jelas, dapat dipahami, dan stabil untuk menghindari keraguan dan kebingungan dalam interpretasi dan penerapan hukum. Dalam hal penafsiran undang-undang, pendekatan sistematis sering digunakan untuk memahami makna yang tidak secara eksplisit dijelaskan dalam peraturan perundang-undangan. Dalam konteks kasus yang Anda sebutkan, penafsiran sistematis digunakan untuk menghubungkan makna pasal yang terkait dengan elemen dari public domain dalam Undang-Undang Nomor 31 Tahun 2000 tentang Desain Industri. Akibat hukum setelah adanya putusan kasasi oleh mahkamah agung terhadap sengketa unsur kebaruan desain industri kemasan produk jahe. Menyatakan Desain Industri Daftar Nomor IDD000040082 tanggal permohonan 12 Maret 2014, dengan judul “KEMASAN” yang dikeluarkan tanggal 19 Juni 2015 atas nama Tergugat bukan Desain Industri yang baru oleh karena telah diumumkan, digunakan dan dipasarkan di Indonesia sejak tahun 2009 jauh sebelum diajukanpermohonan pendaftarannya oleh Tergugat. Membatalkan atau setidak-tidaknya menyatakan batal pendaftaran Desain Industri Daftar Nomor IDD000040082 dengan judul “KEMASAN” dikeluarkan tanggal 19 Juni 2015 atas nama Tergugat dengan segala akibat hukumnya. Bentuk perlindungan hukum pada pemegang hak desain industri terkait dengan kemasan produk jahe dalam putusan 583k/Pdt.Sus-Hki/2021. Memerintahkan pada Direktorat Jenderal Kekayaan Intelektual, dalamhal ini adalah Direktur Hak Cipta dan Desain Industri, mencatat dan mengumumkan pembatalan pendaftaran Desain Industri Daftar Nomor IDD000040082 dengan judul “KEMASAN” Agno: A00201400658 tanggal permohonan 12 Maret 2014 atas nama Tergugat besertadengan segala akibat hukumnya.
Development of Microtik Router Bandwidth Management Applicatio at PLN Office Muara Labuh
Authors: Januardi Cecep , Komala Sari Putri
Published: February 2024

Bandwidth instability is a serious problem where the need for using the internet is in the process, it is necessary to manage bandwidth so that bandwidth is properly organized but changes that will be made to bandwidth management require experts in Mikrotik routers, so an application is needed to manage this bandwidth and can be used by ordinary people so that errors in configuration will be avoided because the configuration is done automatically as desired and can certainly help bandwidth management at a lower cost. Making this application by using data collection methods by taking data at PLN Muara Labuh area, interviewing field supervisors, studying literature that supports problem solving and Unified Modeling Language (UML) for software designers along with software testing. The application is built using django.
Peningkatan Keterampilan Mahasiswa dalam Mempublikasikan Artikel ke Jurnal Terindeks Garuda
Authors: Ekaputra Firdiawan
Published: February 2024

Tujuan – Keterampilan mahasiswa dalam penulisan suatu karya ilmiah harus dimaksimalkan sejak awal perkuliahan. Kemampuan mahasiswa dalam menulis suatu karya ilmiah dapat meningkatkan kemampuan berpikir kritis terhadap fenomena yang terjadi di lingkungan sekitar dan meningkatkan keterampilan menyampaikan suatu gagasan dalam bentuk tulisan ilmiah. Kegiatan pelatihan perlu dilakukan sebagai suatu upaya untuk memfasilitasi mahasiswa dalam menghasilkan suatu karya ilmiah berupa artikel dan mempublikasikan suatu hasil penelitian ke dalam jurnal terindeks garuda. Tujuan dari kegiatan pelatihan ini adalah meningkatan keterampilan mahasiswa dalam mempublikasikan artikel ke dalam jurnal terindeks garuda.

Metode – Kegiatan pelatihan peningkatan keterampilan mahasiswa dalam mempublikasikan artikel ke dalam jurnal terindeks garuda terdiri dari tiga tahap kegiatan yaitu persiapan kegiatan, pelaksanaan pelatihan, dan evaluasi kegiatan. Kegiatan pelatihan dilakukan kepada mahasiswa Program Studi Pendidikan Kimia Universitas Jambi yang berjumlah 26 mahasiswa. Adanya peningkatan keterampilan mahasiswa dalam kegiatan pelatihan diperoleh dari sebaran angket yang diisi oleh mahasiswa peserta pelatihan untuk mengetahui tingkat peningkatan dan ketercapaian pelaksanaan kegiatan pelatihan.

Hasil – Berdasarkan hasil pelatihan yang telah dilaksanakan, menunjukkan adanya peningkatan keterampilan mahasiswa dalam mempublikasikan hasil artikel seperti cara mengoperasikan laman garuda, mengetahui jurnal yang terindeks garuda, cara mengunggah artikel ke dalam jurnal jurnal yang terindeks garuda. Berdasarkan hasil isian angket pada kegiatan evaluasi, diperoleh kriteria sangat baik dengan nilai ketercapaian pelaksanaan kegiatan pelatihan sebesar 4,33.
Penyuluhan Manajemen Waktu Guna Meningkatkan Kedisiplinan Santri di Pondok Pesantren Amanah Bantul
Authors: Putra Hodiri Adi , Makkiyah Nur , Adwia Robbiatul
Published: February 2024

Tujuan - Pendidikan formal berbasis pondok pesantren memiliki instrumen yang lebih kompleks daripada pembinaan di sekolah atau madrasah biasa. Pendampingan dan manajemen waktu untuk membetuk habitat kedesiplinan santri lebih efektif. Tujuan pengabdian ini untuk meningkatkan pengetahuan dan kesadaran santri dalam memenajemen waktu guna meningkatkan kedisiplinan santri dalam dalam mengikuti kegiatan di pondok pesantren.
Metode - kegiatan ini berupa kegiatan penyuluhan kepada santri mengenai manajemen waktu dan kedisiplinan yang dilakukan secara luring sebanyak 64 santri. Media yang digunakan berupa materi power point, laptop, LCD dan kuis online menggunakan aplikasi kahoot. Pengkuruan kedisiplinan santri menggunakan buku mutabaah harian.
Hasil – Berdasarkan hasil dari kegiatan pengabdian ini bahwa para santri sangat antusias mengikuti kegiatan ini dan dapat dengan mudah memahami materi tentang manajemen waktu dan kedisiplinan, serta bisa mengatur diri sendiri dan secara langsung bisa melatih juga mempraktekan kedisiplinan dalam mengikuti kegiatan dipondok pesantren.
Riba dan Bunga Bank Prespektif Majelis Tarjih Muhammadiyah dan Lajnah Bahtsul Masail Nahdlatul Ulama
Authors: Ramadhani Lulu Firdauz , Mahmud Hamidullah
Published: February 2024

Tujuan – Penelitian ini bertujuan untuk mendapatkan kesimpulan atas riba dan bunga bank serta hubungan antara keduanya dengan menggunakan prespektif Majelis tarjih Muhammadiyah dan juga Lajnah Bahtsul Masail Nahdlatul Ulama.

Metode – Penelitian ini menggunakan metode kualitatif yang dapat diartikan sebagai rangkaian proses menjaring informasi suatu objek dan dihubungkan dengan pemecahan suatu masalah, baik dari sudut pandang teoritis maupun praktis.

Hasil – Hasil penelitian ini menunjukkan bahwa Majelis tarjih Muhammadiyah dan Lajnah Bahtsul Masail NU keduanya membedakan antara riba dan bunga bank.
Pengaruh Penambahan Semen Terhadap Stabilisasi Pada Tanah Lempung: Studi Kasus Dusun Lanang Desa Lampasio Kabupaten Tolitoli
Authors: Haris
Published: February 2024

Dalam pelaksanaan konstruksi jalan raya banyak hal yang menjadi permasalahan dilapanganyang salahsatunya adalah adanya daya dukung tanah dasar yang kurang memadai. Olehnya itu perlu dilakukan perbaikan tanah atau Stabilisasi tanah,adapun jenis tanah yang sering mengalami kesulitan dalam perbaikan tanah adalah tanah lempung. Tujuan dilakukannya stabilisasi tanaah adalah untuk mengetahui nilai kepadatan tanah. Tanah lempung merupakan tanah yang buruk memiliki sifat sangat keras dalam keadaan kering dan bersifat plastis pada kadar air sedang. Salah satu contoh tanah yang mengandung lempung yaitu tanah yang berada di Kecamatan Lampasio di Usun Lanang Desa Lampasio Kabupaten Tolitoli, oleh karena itu perlu dilakukan perbaikan tanah melalui stabilisasitanah . Tanah asli yang diberi bahan tambah berupa Portland cement memiliki pengaruh dalam menaikkan nilai kepadatan Compaction dan CBR. Portland cement dapat menurunkan nilai kadar air tanah serta dapat menaikkan nilai MDD dan nilai CBR tanah, penambahan bahan tambah berupa Portland cementterhadap tanah lempung di dapatkan dari jumlah berat contoh tanah kering yg di gunakan untuk pengujian pemadatan standar (Compaction) dan CBR.Secara umum stabilisasi tanah adalah suatu cara yang digunakan untuk mengubah atau memperbaiki sifat tanah pada penelitian ini digunakan metode stabilisasi secara kimiawi, Stabilisai tanah secara kimiawi ialah usaha perbaikan tanah dengan mencampur tanah asli dengan bahan tambah seperti Portlandcement sebagai stabilitator yang bertujuan untuk memperbaiki sifat teknis dari suatu tanah.
Dampak penerapan adaptasi kurikulum pada perubahan sikap siswa dalam aspek kerja sama dan partisipasi di sekolah inklusif
Authors: Aulia Sulthon Wiji Pasha  , Andini Dinar Westri , Sari Dyan Indah Purnama
Published: February 2024

Setiap anak pada dasarnya memiliki karakteristik dan kebutuhan belajar yang berbeda-beda. Namun, sering kali guru menganggap semua anak itu sama. Penelitian ini secara deskriptif bertujuan untuk mendeskripsikan dampak dari penerapan adaptasi kurikulum terhadap perubahan sikap siswa dalam aspek kerja sama dan partisipasi. Penelitian ini merupakan penelitian kualitatif deskriptif dengan menggunakan teknik pemilihan subjek purposive sampling. Sumber data penelitian ini adalah guru kelas VI dan tiga siswa kelas VI. Hasil penelitian menunjukkan bahwa model adaptasi kurikulum telah diterapkan di SDN Demakijo II, dengan melakukan akomodasi berupa pemilihan metode pembelajaran, setting tempat, dan penggunaan dokter untuk modifikasi berupa penurunan indikator dan pemberian soal yang berbeda-beda ketika ulangan harian. Hal tersebut berdampak positif teerhadap kerja sama dan partisipasi siswa yang mana dengan akomodasi dan modifikasi yang dipilih dapat meningkatkan kerja sama dan partisipasi siswa. Dalam proses pembelajaran siswa berperan aktif dengan berdiskusi, saling bantu, bertanya, dan sebagainya. Penelitian ini diharapkan dapat membantu guru mengetahui kemampuan dan potensi masing-masing anak sehingga dapat memperoleh layanan yang sesuai dengan karakteristik dan kebutuhan belajarnya sehingga dapat meningkatkan kerja sama dan partisipasi anak dalam proses pembelajaran.
Evaluating the teratogenic effect of Eugenol in the development of the chick embryos
Authors: Gad El-Hak Heba , Gerges Mariam
Published: February 2024

The aim of the present study is to investigate the effects of single Eugenol dose on chick embryo development. Fertilized treated eggs of Gallus gallus domesticus injected with a single volume of 0.1, 0.2, 0.3, 0.4 and 0.5 μl Eugenol to the egg air chamber and control egg group were incubated at 37ºC in the humidified incubator for 3, 6, 12 and 18 days. A visible damage effect caused by increasing of Eugenol doses on the vascular development with the cause of angiogenesis and brain acetychlorinesterase (AchE) activity suppression, incomplete embryonic development with the increasing of malformation and the mortality rate of the chick embryo. This work demonstrated that the treatment of the chick embryo in the early stages with Eugenol may be considered as teratogenic agents at the dose levels utilized in our laboratory conditions between days 3 to day 18 of incubation.
Insecticidal activity of Xylopia aethiopica (Family; Annonaceae) against Callosobruchus maculatus (F) (Coleoptera: Bruchidae) and Sitophilus oryzae (Coleoptera: Curculionidae)
Authors: Ito Edwin , Ukpohwo Regina , Okiriguo Ifeoma
Published: February 2024

The efficacy of Xylopia aethiopica leaf dust and extract on Callosobruchus maculatus and Sitophilus oryzae was evaluated at different doses (1.0 – 3.0 g and 50.0-100 mg-ml) with 10 unsexed adult weevils per 10 gram of substrate per replicate. All treatments were triplicated and mortality of the insects was recorded after every 24 hours (h) for 96 h exposure to powder and extract respectively. The parameter compared was the mortality rate of the adult pests. The negropepper was an active biopesticide against C. maculatus and S. oryzae. However, the plant products gave higher mortality on S. oryzae with a mean of mean mortality and LD50 of 82.2% and 1.06g respectively than on C. maculatus whose mean mortality and LD50 was 79.9% and 1.12g respectively over 96 hours exposure. Statistical analysis showed a significant difference (P<0.05) in pest mortality between treated and control samples. The results suggested that X. aethiopica is more promising botanical insecticides on S. oryzae than C. maculatus.
Thyroid activity of Emballonurid male bat, Taphozous longimanus (Hardwicke) during reproductive period
Authors: Abaji Shende Virendra
Published: February 2024

Thyroid gland in bat, Taphozous longimanus consists of two lateral lobes on the either side of trachea and are connected by isthmus. During the pre-breeding period large and medium sized follicles are predominant and small sized follicles are few while during the breeding period small and medium sized follicles are much more and large sized follicles are a few. Thyroid gland is composed of two different kinds of parenchymal cells, follicular epithelial cells and parafollicular cells (‘C’ cells or Calcitonin secreting cells or basal granular cell).  ‘A’ type of follicular cells is lined by tall columnar epithelial cells which are highly secretory in nature, ‘B’ type of follicular cells is lined by low columnar epithelial cells and are secretory in nature while ‘C’ type of follicular cells is lined by low cuboidal epithelial cells. Parafollicular cells during the breeding period are more in number and larger in size than the pre-breeding period. During the breeding period, weight of thyroid gland, the mean diameter of follicles, colloid and height of epithelial cells of small, medium and large sized follicles are more, but not significantly different from that of the pre-breeding period.
Geometric morphometrics of the head shape of Pseudocerastes persicus (DUMÉRIL, BIBRON & DUMÉRIL, 1854) (Ophidia: Viperidae) from central Iran
Authors: Belgheiszadeh Hamid , Salemi Ali , Heydari Nastaran , Tahouri Ayda , Tafreshi Sogol
Published: February 2024

In the present study, we focused on three populations of Persian Horned Snake, Pseudocerastes persicus from Iran and examined them morphologically. Geometric morphometrics is an appropriate method to evaluate the scale positions and shape in different populations of a given species in snakes. We used this method to evaluate the geographic variation among three populations of Pseudocerastes persicus. Based on variance and multivariate analyses, all populations from Isfahan, Semnan and Markazi provinces don’t show variation in head scales. This result from morphological features confirmed the previous molecular study on these populations. We recommended examining further taxonomic characters of these populations as karyotype and allozyme features.
First report of Alternaria phragmospora causing early blight disease of Lycopersicon esculentum in Egypt
Authors: Abdel-Motaal Fatma , Kamel Noha , El-Zayat Soad , Abou-Ellail Mohamed
Published: February 2024

Tomato is one of the most common vegetable crops in the world. Many diseases would infect tomatoes during Tomato the growing season. In summer (August, 2018), from different fields (5 fields) severe brown spots appeared on the leaves, stems, and fruits of Lycopersicon esculentum variety Castle rock in the Abu El-Rish region, at Aswan city, Upper Egypt. The pathogen which isolated from different infected parts of the plant was identified as Alternaria phragmospora according to the morphological and molecular characterization using primers ITS1 and ITS4. The pathogenicity was tested by the re-inoculation of the healthy tomato plant from the same variety either by spore suspension (1.2x106 conidia/ ml) spraying or direct mycelium inoculum on the plant surface. Virulent symptoms were observed in the inoculated plant. In the lab, moisture and high temperature increase the disease severity of infected plant while in the field, high temperature (40℃-47℃), excess watering of the plant and condensation of them in patches were recognized and these conditions increased the disease severity and spreading from plant to other. To our knowledge, this is the first detection of the pathogen, A. phragmospora which infected L. esculentum.
Mises en présence du végétal et pratiques attentionnelles dans Branché et We Move Together Or Not At All
Authors: Cyr Catherine
Published: February 2024

Cet article porte sur l’expérience de réception de deux oeuvres performatives récentes s’attachant à mettre en relation des êtres humains et des végétaux : le spectacle ambulatoire Branché, des compagnies circassiennes Acting for Climate – Montréal et Barcode et l’installation chorégraphique de longue durée We Move Together Or Not At All de Sasha Kleinplatz. Par le biais d’une perspective soma-esthétique, qui fait du corps sentant et ressentant le site d’élaboration de la pensée, la réflexion examine la façon dont la rencontre avec le végétal et avec les autres composantes de l’environnement détermine et affecte l’expérience spectatorielle. Pour rendre compte de la dimension sensible de celle-ci, l’autrice privilégie, en partie, une méthodologie auto-ethnographique où des fragments tirés de son carnet d’observation, ressaisis par l’écriture, sont intégrés à l’analyse. Opérant un maillage théorique entre les champs des études en arts vivants, de l’écocritique, de la philosophie environnementale et des Critical Plant Studies, l’article place en son centre les effets et les affects induits par l’agentivité et la bioperformativité des végétaux en régime performatif. Il s’intéresse au déplacement et à l’intensification des pratiques attentionnelles et à la dimension éminemment politique de cette reconfiguration.
Corps et plantes : porter ou adopter
Authors: Weber Pascale
Published: February 2024

Cet article présente ce qui a conduit le duo Hantu (Weber+Delsaux) à créer des mises en scène et actions performatives où il est essentiellement question de se déplacer chargés de réceptacles végétalisés. De tels dispositifs permettent d’interroger le geste du portage. Nous portons ce que nous partageons, un héritage, des revendications, des espoirs de changements… Ce geste atteste également de liens affectifs et de nouvelles socialités que nous pourrions développer avec les plantes. Mais le portage peut également conforter l’idée que nous avons le pouvoir de manipuler, déplacer, arranger le végétal à notre guise, renvoyant une vision passive du végétal. L’article développe alors en contrepoint du portage le principe d’adoption végétale, qui permettrait d’envisager la plante comme sujet vivant, de reconnaître son agentivité et de faire l’hypothèse d’une relation partagée, car l’adoption implique un mutuel engagement. À partir d’exemples d’expériences, de performances, de workshops menés par le duo, ce texte s’efforce de saisir dans quelle mesure le principe d’adoption est transposable au monde végétal et ce que signifie respecter, prendre en compte et en charge une plante, et réciproquement.
The Politics of “Pombilai Orumai”: The 2015 Kanan Devan Strike in Kerala, India
Authors: S. Anagha
Published: February 2024

Colonial hegemony was retained in the South Indian plantations of Kanan Devan Hills Plantations Limited, where the workers belonged to marginalized classes. The landless employees were given housing facilities, and this compelled them to remain there for generations despite poor wages. These uneducated and geographically secluded people found it difficult to come out of the plantation labyrinth, and the labour acts or land legislation acts were not much help. In 2015, around 5,000 women workers called “Pombilai Orumai” led a successful strike for a wage increase. The most remarkable aspect of this was the disassociation with political parties and trade unions and the solidarity of women workers despite all odds.
Sensitization of meningeal afferents to locomotion-related meningeal deformations in a migraine model
Authors: Blaeser Andrew S , Zhao Jun , Sugden Arthur U , Carneiro-Nascimento Simone , Andermann Mark L , Levy Dan
Published: February 2024

Migraine headache is hypothesized to involve the activation and sensitization of trigeminal sensory afferents that innervate the cranial meninges. To better understand migraine pathophysiology and improve clinical translation, we used two-photon calcium imaging via a closed cranial window in awake mice to investigate changes in the responses of meningeal afferent fibers using a preclinical model of migraine involving cortical spreading depolarization (CSD). A single CSD episode caused a seconds-long wave of calcium activation that propagated across afferents and along the length of individual afferents. Surprisingly, unlike previous studies in anesthetized animals with exposed meninges, only a very small afferent population was persistently activated in our awake mouse preparation, questioning the relevance of this neuronal response to the onset of migraine pain. In contrast, we identified a larger subset of meningeal afferents that developed augmented responses to acute three-dimensional meningeal deformations that occur in response to locomotion bouts. We observed increased responsiveness in a subset of afferents that were already somewhat sensitive to meningeal deformation before CSD. Furthermore, another subset of previously insensitive afferents also became sensitive to meningeal deformation following CSD. Our data provides new insights into the mechanisms underlying migraine, including the emergence of enhanced meningeal afferent responses to movement-related meningeal deformations as a potential neural substrate underlying the worsening of migraine headache during physical activity.
PCBP2 as an intrinsic aging factor regulates the senescence of hBMSCs through the ROS-FGF2 signaling axis
Authors: Chen Pengbo , Li Bo , Lu Zeyu , Xu Qingyin , Zheng Huoliang , Jiang Shengdan , Jiang Leisheng , Zheng Xinfeng
Published: February 2024

It has been reported that loss of PCBP2 led to increased reactive oxygen species (ROS) production and accelerated cell aging. Knockdown of PCBP2 in HCT116 cells leads to significant down-regulation of fibroblast growth factor 2 (FGF2). Here, we tried to elucidate the intrinsic factors and potential mechanisms of BMSCs aging from the interactions among PCBP2, ROS and FGF2.Unlabeled quantitative proteomics were performed to show differentially expressed proteins in the replicative senescent human-derived bone marrow stromal cells (RS-hBMSCs). ROS and FGF2 were detected in the loss-and-gain cell function experiments of PCBP2. The function recovery experiments were performed to verify whether PCBP2 regulates cell function through ROS/FGF2-dependent ways.PCBP2 expression was significantly lower in P10-hBMSCs. Knocking down the expression of PCBP2 inhibited the proliferation while accentuated the apoptosis and cell arrest of RS-hBMSCs. PCBP2 silence could increase the production of ROS. On the contrary, overexpression of PCBP2 increased the viability of both P3-hBMSCs and P10-hBMSCs significantly. Meanwhile, over-expression of PCBP2 led to significantly reduced expression of FGF2. Overexpression of FGF2 significantly offset the effect of PCBP2 overexpression in P10-hBMSCs, leading to decreased cell proliferation, increased apoptosis and reduced G0/G1 phase ratio of the cells.This study initially elucidates that PCBP2 as an intrinsic aging factor regulates the replicative senescence of hBMSCs through the ROS-FGF2 signaling axis.
Hemispheric divergence of interoceptive processing across psychiatric disorders
Authors: Adamic Emily M , Teed Adam R , Avery Jason , de la Cruz Feliberto , Khalsa Sahib
Published: February 2024

Interactions between top-down attention and bottom-up visceral inputs are assumed to produce conscious perceptions of interoceptive states, and while each process has been independently associated with aberrant interoceptive symptomatology in psychiatric disorders, the neural substrates of this interface are unknown. We conducted a preregistered functional neuroimaging study of 46 individuals with anxiety, depression, and/or eating disorders (ADE) and 46 propensity-matched healthy comparisons (HC), comparing their neural activity across two interoceptive tasks differentially recruiting top-down or bottom-up processing within the same scan session. During an interoceptive attention task, top-down attention was voluntarily directed towards cardiorespiratory or visual signals. In contrast, during an interoceptive perturbation task, intravenous infusions of isoproterenol (a peripherally-acting beta-adrenergic receptor agonist) were administered in a double-blinded and placebo-controlled fashion to drive bottom-up cardiorespiratory sensations. Across both tasks, neural activation converged upon the insular cortex, localizing within the granular and ventral dysgranular subregions bilaterally. However, contrasting hemispheric differences emerged, with the ADE group exhibiting (relative to HCs) an asymmetric pattern of overlap in the left insula, with increased or decreased proportions of co-activated voxels within the left or right dysgranular insula, respectively. The ADE group also showed less agranular anterior insula activation during periods of bodily uncertainty (i.e. when anticipating possible isoproterenol-induced changes that never arrived). Finally, post-task changes in insula functional connectivity were associated with anxiety and depression severity. These findings confirm the dysgranular mid-insula as a key cortical interface where attention and prediction meet real-time bodily inputs, especially during heightened awareness of interoceptive states. Furthermore, the dysgranular mid-insula may indeed be a ‘locus of disruption’ for psychiatric disorders.
Speech and music recruit frequency-specific distributed and overlapping cortical networks
Authors: te Rietmolen Noémie , Mercier Manuel R , Trébuchon Agnès , Morillon Benjamin , Schön Daniele
Published: February 2024

To what extent does speech and music processing rely on domain-specific and domain-general neural networks? Using whole-brain intracranial EEG recordings in 18 epilepsy patients listening to natural, continuous speech or music, we investigated the presence of frequency-specific and network-level brain activity. We combined it with a statistical approach in which a clear operational distinction is made between shared, preferred, and domain-selective neural responses. We show that the majority of focal and network-level neural activity is shared between speech and music processing. Our data also reveal an absence of anatomical regional selectivity. Instead, domain-selective neural responses are restricted to distributed and frequency-specific coherent oscillations, typical of spectral fingerprints. Our work highlights the importance of considering natural stimuli and brain dynamics in their full complexity to map cognitive and brain functions.
Coevolution with toxic prey produces functional trade-offs in sodium channels of predatory snakes
Authors: del Carlo Robert E. , Reimche Jessica S. , Moniz Haley A. , Hague Michael T.J. , Agarwal Shailesh R. , Brodie Edmund D. , Brodie Edmund D. , Leblanc Normand , Feldman Chris R.
Published: February 2024

Seemingly unrelated traits often share the same underlying molecular mechanisms, potentially generating a pleiotropic relationship whereby selection shaping one trait can simultaneously compromise another. While such functional trade-offs are expected to influence evolutionary outcomes, their actual relevance in nature is masked by obscure links between genotype, phenotype, and fitness. Here, we describe functional trade-offs that likely govern a key adaptation and coevolutionary dynamics in a predator-prey system. Several garter snake (
Thamnophis
spp.) populations have evolved resistance to tetrodotoxin (TTX), a potent chemical defense in their prey, toxic newts (
Taricha
spp.). Snakes achieve TTX resistance through mutations occurring at toxin-binding sites in the pore of snake skeletal muscle voltage-gated sodium channels (Na
V
1.4). We hypothesized that these mutations impair basic Na
V
functions, producing molecular trade-offs that should ultimately scale up to compromised organismal performance. We investigate biophysical costs in two snake species with unique and independently evolved mutations that confer TTX resistance. We show electrophysiological evidence that skeletal muscle sodium channels encoded by toxin-resistant alleles are functionally compromised. Furthermore, skeletal muscles from snakes with resistance genotypes exhibit reduced mechanical performance. Lastly, modeling the molecular stability of these sodium channel variants partially explains the electrophysiological and muscle impairments. Ultimately, adaptive genetic changes favoring toxin resistance appear to negatively impact sodium channel function, skeletal muscle strength, and organismal performance. These functional trade-offs at the cellular and organ levels appear to underpin locomotor deficits observed in resistant snakes and may explain variation in the population-level success of toxin-resistant alleles across the landscape, ultimately shaping the trajectory of snake-newt coevolution.
Absenteísmo e eficiência: um estudo nas instituições federais de ensino superior
Authors: Mendes Nara Cristina Ferreira , Serrano André Luiz Marques , Matias-Pereira José , Cruz Emelle Rodrigues Novais , Costa Abimael de Jesus Barros
Published: February 2024

Esta pesquisa pretende identificar a relação existente entre absenteísmo e eficiência no contexto das Instituições Federais de Ensino Superior (IFES’s). Para tanto, o estudo utilizou a metodologia de Análise Envoltória de Dados (DEA). Na execução desse método desconsideramos o absenteísmo (variável interesse), no primeiro momento, que resultou em 15 IFES’s como destaque em benchmark e, em seguida, ao considerando o efeito da variável absenteísmo o resultado alterou-se, pois das 15 instituições identificadas inicialmente como referência, apenas 2 delas permaneceram como destaque. Sendo assim, os resultados permitem concluir que o absenteísmo afeta negativamente na eficiência das instituições de educação superior do setor público.
Analysis of slave labor in the meatpacking sector
Authors: Kuazaqui Edmir , Lisboa Teresinha Covas
Published: February 2024

This article aims to present a category of activity – slavery, where there is the subletting of workers without a legal employment relationship, without due remuneration as well as labor rights and responsibilities. In this way, companies that practice this type of activity reduce their operational labor costs, payment of taxes and increase the profit margin, bringing economic and mainly social impacts to both the worker and society, a qualitative reserarch with the meatpacking segment, one of the sectors in the country where part of this harmful practice converges in society.
Comparison of online to face-to-face instruction for anatomy review in a third-year clinical course
Authors: Faydenko Jocelyn , Grieve Thomas , Madigan Dana , Pocius Judith D. , Olsen Christopher , Cramer Gregory D.
Published: February 2024

Objective

This project compared student learning and satisfaction of an anatomy review delivered by a face-to-face lecture (F2FL) and an online learning module (OLM) for third-year doctor of chiropractic students.

Methods

This cohort study compared student learning and satisfaction of a pediatric spinal anatomy review delivered via F2FL (cohort 1, n = 23) and OLM (cohort 2, n = 18) in 2 successive 2019 (pre-COVID) course offerings. Previously validated pre- and post-tests were given. Students completed a survey assessing delivery, comfort with online learning and online learning technology, and preference of F2FL vs OLM of review material. Pre- and post-test results were assessed using repeated-measures analysis of variance.

Results

Testing results showed an improvement with both groups (F2FL 53.7%, p < .001 vs OLM 51.8%, p < .001), with no significant difference between the F2FL and OLM groups (p = .53; p = .82). The survey showed: 83.3% of OLM students felt the online method was effective, and 88.9% of the OLM students would prefer online reviews or have no preference between online or face-to-face; meanwhile, 80% of the F2FL group thought the lecture engaging/effective, whereas 60% of the F2FL group would have preferred to have the material presented online.

Conclusion

The OLM was found to be as effective as the F2FL for the content assessed. The majority of students would prefer the online method for future anatomy review content presented in the course. This strategy could be applied to provide review materials in other clinical courses, allowing material to be developed and given by content experts while freeing valuable in-class time.
Biostimulants for Promoting Growth, Yield and Flower Quality in Anthurium andreanum Lind
Authors: Thomas Jewel Maria , Reshmi CR  , Rafeekher M  , Priyakumari I  , Aparna B
Published: February 2024

The present study was carried out at the Department of Floriculture and Landscaping, College of Agriculture, Vellayani, Kerala Agricultural University, during 2022-23 with an aim to evaluate the effect of foliar application of different biostimulants on growth, yield, and flowering attributes of Anthurium under naturally ventilated polyhouse condition. The experiment was laid out in CRD with ten replications. There were fifteen treatments including the control.  Fertilizers and manures were applied as per the Package of Practices Recommendations of Kerala Agricultural University (i.e., application of cow dung supernatant + 2 g L-119:19:19 weekly once) to all the treatments including the control (T1). In addition, for treatments T2 to T15, different biostimulants were applied as foliar sprays either alone or in combination fortnightly or monthly as per the treatment specifications. Being a popular cut flower, improvement in floral attributes is very much valued in Anthurium. The study concluded that the treatment T8 i.e., application of cow dung supernatant and 2 g L-119:19:19 weekly once as foliar spray along with foliar application of   2% humic acid - fulvic acid mixture and 100 mg L-1 salicylic acid at fortnightly intervals exhibited superior values for stalk length, spathe length, spathe width, number of flowers, flower longevity and vase life and it also recorded earliest flower bud initiation. The results revealed that growth and floral attributes of Anthurium can be improved through the foliar application of treatment T8.
Antipsychotics in child and adolescent patients with major depressive disorder: A retrospective analysis of prescribing patterns
Authors: Dauchot Danielle , Rettey Suzanne , Melton Brittany L. , Moeller Karen E.
Published: February 2024

Introduction

Depression rates in children/adolescents in the United States have increased in the last 10 years. Fluoxetine and escitalopram are the only 2 antidepressants approved for the treatment of major depression disorder (MDD) in children/adolescents. In adults, some antipsychotics are approved for augmented treatment of MDD. However, there is limited research on antipsychotic augmentation in child/adolescent MDD.

Methods

This retrospective chart review evaluated antipsychotic prescribing for MDD in hospitalized patients aged 4 to 17 years to determine the frequency of prescribing antipsychotics for MDD and what factors influence the addition of an antipsychotic. For inclusion, patients were diagnosed with MDD and not on an antidepressant or antipsychotic before admission. Binomial logistic regression was used to analyze variables with prescribed antipsychotics as the dependent variable.

Results

There were 6.8% of patients prescribed an antipsychotic. Binomial logistic regression analysis found that increased age (odds ratio [OR] 1.28; 95% CI = 1.045, 1.568; P = .017) and multiple admissions within 1 year (OR 3.277; 95% CI = 2.283, 4.705; P < .001) were associated with the use of antipsychotics in patients with MDD. Posttraumatic stress disorder and disruptive mood dysregulation disorder were also associated with the use of antipsychotics.

Discussion

Careful consideration should be taken when using off-label antipsychotics in children due to limited studies on efficacy. Future research is warranted to assess the efficacy and safety of these agents in children and adolescents.
Clinical pharmacist intervention to ensure safe stimulant prescribing practices at a Veterans Affairs facility
Authors: Bass Bailey B. , Vann Lacey J.
Published: February 2024

Introduction

The Psychotropic Drug Safety Initiative (PDSI) is a national Veterans Affairs program that recommends obtaining cardiovascular vital signs semiannually and urine toxicology screening annually for veterans prescribed stimulants. The PDSI also recommends a risk review of concurrent central nervous system (CNS) depressants to ensure the benefits of coadministration with stimulants outweigh the risks. This project’s purpose was to evaluate the occurrence of coprescriptions for CNS depressants and stimulants and encourage compliance with the PDSI recommendations to increase safe and appropriate management of veterans prescribed the combination. This study aimed to evaluate the occurrence of coprescriptions for CNS depressants and stimulants, evaluate compliance with stimulant monitoring recommendations, and measure the proportion of pharmacist recommendations implemented by the prescriber.

Methods

This quality improvement project identified veterans with an outpatient prescription for a stimulant and any coprescription(s) for benzodiazepines, sedative-hypnotics, and/or opioids. A pharmacy intervention note was generated to request a risk review, provide recommendations for de-escalation, and notify the stimulant prescriber of overdue monitoring parameters. Impact was measured 60 days after intervention. Descriptive statistics and a McNemar test were used to compare preintervention and postintervention data.

Results

From the 61 patients included, there were 67 unique prescriptions for benzodiazepines (49.3%), sedative-hypnotics (34.3%), and opioids (16.4%) in combination with a stimulant. Pharmacist intervention resulted in de-escalation of coprescribing for 9 patients (16.1%) and was associated with statistically significant improvement in compliance to stimulant monitoring recommendations.

Discussion

Clinical pharmacists can assist in ensuring safe and appropriate monitoring and management of veterans prescribed stimulants.
Serotonin syndrome and neuroleptic malignant syndrome: A case report of intersecting symptomatology
Authors: Maktabi Loulwa , Shipman Denver , Reinert Justin P.
Published: February 2024

Introduction

Serotonin syndrome and neuroleptic malignant syndrome are caused by 2 distinct pathologies; however, the clinical presentation associated with both syndromes share many features.

Methods

We describe a 56-year-old male patient who presented to our facility with seizures, leukocytosis, fevers, extremity hyperreflexia, and signs of autonomic dysfunction as evidenced by cardiovascular instability. The patient was noted to be taking vortioxetine, trazodone, lamotrigine, lurasidone, and carbidopa-levodopa as outpatient medications for his depression, an unspecified mood disorder, and Parkinson disease. Following a robust workup and failure of other therapies, all serotonergic and dopaminergic medications were held, and the patient was tried on cyproheptadine for serotonin syndrome, which led to the cessation of fevers. Bromocriptine was added to the regimen, which led to the resolution of the remainder of the patient’s symptoms.

Results

The overlapping symptomatology of several key diagnostic criteria for both serotonin syndrome and neuroleptic malignant syndrome as well as their nature as diagnoses of exclusion require an evaluation of the patient’s aggregate improvement following targeted pharmacologic strategies for both syndromes. The efficacy of both cyproheptadine and bromocriptine when administered concomitantly support the concurrent pathologies.

Discussion

Clinicians at the bedside must be cognizant of the potential for clinically relevant drug-drug interactions that may present with overlapping pathologies.
Valproate, risperidone, and paliperidone: A case of valproate-induced hyperammonemic encephalopathy
Authors: Wesselman Kyle , Cavaliere Vincent , Goyal Rakesh , Anderson Eric
Published: February 2024

Hyperammonemia is a well-known adverse effect of valproate that can progress to a potentially fatal condition known as valproate-induced hyperammonemic encephalopathy (VHE). VHE is more common when valproate is used in combination therapy with other antiepileptic medications. A growing number of case reports have pointed to a possible interaction with the antipsychotic risperidone leading to an increased risk of VHE. We present a case of VHE in which a 20-year-old male patient with bipolar affective disorder developed VHE when on concomitant valproate, risperidone, and paliperidone palmitate. On the seventh day of treatment with oral risperidone, oral divalproex sodium was added. Intramuscular paliperidone palmitate was initiated on day 13, and oral risperidone was discontinued after the second loading dose on day 16. The following day, the patient displayed worsening psychomotor retardation, swaying gait, drowsiness, and vomiting. The patient was found to have hyperammonemia and transferred to the emergency department for treatment of suspected VHE.
Detection of a Plasmodium vivax erythrocyte binding protein by flow cytometry
Authors: Tran Tuan M. , Moreno Alberto , Yazdani Syed S. , Chitnis Chetan E. , Barnwell John W. , Galinski Mary R.
Published: December 2004

Background

The malaria parasite Plasmodium vivax preferentially invades reticulocytes. It is therefore relevant for vaccine development purposes to identify and characterize P. vivax proteins that bind specifically to the surface of reticulocytes. We have developed a two‐color flow cytometric erythrocyte binding assay (F‐EBA) that has several advantages over traditional erythrocyte binding assays (T‐EBAs) used in malaria research. We demonstrate the use of F‐EBA using the P. vivax Duffy binding protein region II (PvDBP‐RII) recombinant protein as a model. This protein binds to all erythrocytes that express the Duffy receptor (Fy) and discriminates binding between normocytes and reticulocytes.

Methods

F‐EBAs were performed by incubating freshly isolated Aotus nancymai, Macaca mulatta, Saimiri boliviensis, and human erythrocytes with PvDBP‐RII, a fluorescent anti‐His tag detection antibody, and thiazole orange before flow cytometric analysis. T‐EBAs employing immunoblot detection with an anti‐His antibody were performed concomitantly.

Results

PvDBP‐RII bound to A. nancymai, M. mulatta, and human Fy+ erythrocytes, but not human Fy− erythrocytes, by F‐EBAs and T‐EBAs. However, F‐EBAs exhibited higher sensitivity and better concordance between experiments compared with T‐EBAs.

Conclusions

F‐EBA is a rapid, simple, and reliable method for quantifying the ability of malaria proteins to bind to the surface of erythrocytes. F‐EBA can discriminate binding between erythrocyte subpopulations without enrichment protocols and may be more reliable and sensitive than T‐EBAs in identifying novel erythrocyte binding proteins. © 2004 Wiley‐Liss, Inc.