bluuebunny/crossref_metadata_2025 huggingface dataset:
Can the Forestry Sector in Jiangsu Province Gain Competitiveness Under Impoverished Forest Resources?
Authors: Zeng Jiejie , Lin Weiting , Kong Fanbin
Published: January 2025

Jiangsu province’s forestry industry offers valuable insights for resource-poor regions in China. This study aims to determine the competitiveness of different forestry sectors in Jiangsu province and identify the industrial structural changes in this context. The shift-share method is employed to analyze the primary, secondary, and tertiary forestry industries, encompassing 17 sub-sectors, and investigate the industrial regional types. By combining location quotient values and inter-sector characteristics, the leading sectors are identified. The findings reveal that the Kuznets facts and the Engel effect in the industry structure have facilitated the process of increasing the value of ecological products; this is particularly evident between 2002 and 2018, as well as in 2021. Within the primary forestry industry, the Flower cultivation sector and the Economic forest product sector emerge as the leading sectors, outperforming the national growth effect by 7.18 and 1.02 times, respectively. In the secondary forestry industry, the Wood processing sector, the Furniture manufacturing sector, the Wood paper-making sector, and the Non-timber forest product sector mostly show the Progressive types as the leading sectors from 2006 to 2020. The tertiary industry’s regional types fluctuate from the Progressive type to the Medium type, with the Forestry tourism sector, the Ecological service sector, and the Non-forestry service sector displaying leading industry characteristics. Recommendations for enhancing inter-sector integration include fostering collaborative agglomeration, unlocking the potential value of wetland resources, and strengthening cross-industry integration through e-commerce clusters to improve the forester’s e-commerce operational capabilities.
Ballistic Impact Study of an Aramid Fabric by Changing the Projectile Trajectory
Authors: Titire Larisa , Muntenita Cristian
Published: January 2025

Personal protective systems widely use aramid textile fabrics, whether in soft or rigid form, to protect against various types of ballistic threats. Ballistic impact refers to a high-velocity impact caused by a thrusting source, often involving a low-mass object. To use these materials effectively in structural applications, it is crucial to have a thorough understanding of their ballistic behavior when subjected to high-velocity impact. Upon contact of the projectile with the ballistic material, complex ballistic penetration processes take place, which require a comprehensive and quantitative examination for a better understanding. This study aims to analyze the damage mechanism of aramid fabric by altering the projectile impact trajectory based on numerical simulations. We aim to obtain a thorough understanding of the behavior of the aramid fabric by performing numerical simulations and examining the penetration process in detail. The obtained results are analyzed based on the von Mises stress distribution (panel and projectile, projectile only, and main wires), projectile deformation, projectile velocity during ballistic impact, and based on photographs obtained during the impact.
Lactic Acid Bacteria from Northern Thai (Lanna) Fermented Foods: A Promising Source of Probiotics with Applications in Synbiotic Formulation
Authors: Suwannasom Nittiya , Siriphap Achiraya , Japa Ornampai , Thephinlap Chonthida , Thepmalee Chutamas , Khoothiam Krissana
Published: January 2025

Northern Thai culture offers a rich variety of traditional fermented foods beneficial for gastrointestinal health. In this study, we characterized lactic acid bacteria (LAB) from various indigenous fermented foods as potential probiotic candidates and determined their properties for application in commercial synbiotic formulation. Five isolates demonstrating high tolerance to low pH (2.0) and 0.3% bile salts were collected and characterized. These included three strains of Lactiplantibacillus plantarum isolated from nham (NB1, NP2, and NP11) and two strains of Limosilactobacillus fermentum isolated from pla-som (PS4 and PS7). All the selected LAB isolates exhibited γ-hemolytic activity, strong antimicrobial activity, and high resistance to gastric and duodenal digestion conditions. Among the LAB isolates, L. plantarum NB1 demonstrated the highest capacity for adhesion to Caco-2 cells, auto-aggregation, and antioxidant activity, differing significantly (p < 0.05) from the other isolates. Furthermore, the NB1 strain exhibited preferential growth in the presence of commercial prebiotics (fructooligosaccharide, lactose, and inulin) and good survival after lyophilization, which is a desirable characteristic for a powdered ingredient. Therefore, the NB1 strain is a suitable probiotic candidate for applications in synbiotic formulation or as a functional food ingredient.
Machine Learning and Multi-Omics Integration to Reveal Biomarkers and Microbial Community Assembly Differences in Abnormal Stacking Fermentation of Sauce-Flavor Baijiu
Authors: Li Shuai , Han Yueran , Yan Ming , Qiu Shuyi , Lu Jun
Published: January 2025

Stacking fermentation is critical in sauce-flavor Baijiu production, but winter production often sees abnormal fermentations, like Waistline and Sub-Temp fermentation, affecting yield and quality. This study used three machine learning models (Logistic Regression, KNN, and Random Forest) combined with multi-omics (metagenomics and flavoromics) to develop a classification model for abnormal fermentation. SHAP analysis identified 13 Sub-Temp Fermentation and 9 Waistline microbial biomarkers, along with 9 Sub-Temp Fermentation and 12 Waistline flavor biomarkers. Komagataeibacter and Gluconacetobacter are key for normal fermentation, while Ligilactobacillus and Lactobacillus are critical in abnormal cases. Excessive acid and ester markers caused unbalanced aromas in abnormal fermentations. Additionally, ecological models reveal the bacterial community assembly in abnormal fermentations was influenced by stochastic factors, while the fungal community assembly was influenced by deterministic factors. RDA analysis shows that moisture significantly drove Sub-Temp fermentation. Differential gene analysis and KEGG pathway enrichment identify metabolic pathways for flavor markers. This study provides a theoretical basis for regulating stacking fermentation and ensuring Baijiu quality.
Impact of Enzymatic Degradation Treatment on Physicochemical Properties, Antioxidant Capacity, and Prebiotic Activity of Lilium Polysaccharides
Authors: Peng Kaitao , Zhang Yujie , Zhang Qi , Wang Yunpu , Liu Yuhuan , Cui Xian
Published: January 2025

In order to overcome the bioavailability limitation of Lilium polysaccharide (LPS) caused by its high molecular weight and complex structure, two low-molecular-weight degraded polysaccharides, namely G-LPS(8) and G-LPS(16), were prepared through enzymatic degradation. The molecular weight of LPS was significantly reduced by enzymolysis, leading to increased exposure of internal functional groups and altering the molar ratio of its constituent monosaccharides. The results of antioxidant experiments showed that enzymatic hydrolysis had the potential to enhance the antioxidant performance of LPS. In vitro fermentation experiments revealed that LPS and its derivatives exerted different prebiotic effects on intestinal microbial communities. Specifically, LPS mainly inhibited the growth of harmful bacteria such as Fusobacterium, while G-LPS(8) and G-LPS(16) tended to promote the growth of beneficial bacteria like Megamonas, Bacteroides, and Parabacteroides. Metabolomic analysis revealed that LPSs with varying molecular weights exerted comparable promoting effects on multiple amino acid and carbohydrate metabolic pathways. Importantly, with the reduction in molecular weight, G-LPS(16) also particularly stimulated sphingolipid metabolism, nucleotide metabolism, as well as ascorbic acid and uronic acid metabolism, leading to the significant increase in specific metabolites such as sphingosine. Therefore, this study suggests that properly degraded LPS components have greater potential as a prebiotic for improving gut health.
A Review on Blockchain Applications in Operational Technology for Food and Agriculture Critical Infrastructure
Authors: Zheng Chengliang , Peng Xiangzhen , Wang Ziyue , Ma Tianyu , Lu Jiajia , Chen Leiyang , Dong Liang , Wang Long , Cui Xiaohui , Shen Zhidong
Published: January 2025

The food and agriculture sector is a cornerstone of critical infrastructure (CI), underpinning global food security, public health, and economic stability. However, the increasing digitalization and connectivity of operational technologies (OTs) in this sector expose it to significant cybersecurity risks. Blockchain technology (BT) has emerged as a transformative solution for addressing these challenges by enhancing network security, traceability, and system resilience. This study presents a comprehensive review of BT applications in OT security for food and agriculture CI, employing bibliometric and content analysis methods. A total of 124 relevant articles were identified from six databases, including the Web of Science Core Collection and MEDLINE®. Bibliometric analysis was conducted across five dimensions: publication year, literature type, journal distribution, country contributions, and keyword trends. The findings are meticulously organized through tables, charts, and graphs. The year 2018 marked a surge in research within this domain, with the IEEE Internet of Things Journal and IEEE ACESS emerging as the most prolific journals, each boasting nine publications. The United States, China, and India are at the forefront in terms of journal citation counts. Our analysis determined that a reference count of 37 serves as an appropriate threshold. Otoum Safa stands out as the author with the highest number of published articles, totaling four. Keywords such as “blockchain”, “internet of things”, “smart contract”, “security”, and “critical infrastructure” appear with significant frequency. The statistics, trends, and insights gleaned from this bibliometric analysis can guide researchers in the OTCI field to forge a coherent and logical research trajectory. Content analysis further identified six key research areas within this domain: identity authentication and data verification, secure access control, attack detection and perception, data security and protection, data backup and recovery, and attack assessment and attribution. Based on these insights, a general framework is proposed to guide future research and practical applications of BT in securing OT within food and agriculture CI. This study systematically analyzes the current research landscape, challenges, and opportunities for BT in securing the OT critical to food and agriculture CI. By bridging the gap between blockchain innovations and the operational needs of the food and agriculture sector, this work contributes to advancing strategic implementation and improving the security of CI systems.
Finite-Time Cluster Synchronization of Fractional-Order Complex-Valued Neural Networks Based on Memristor with Optimized Control Parameters
Authors: Chang Qi , Wang Rui , Yang Yongqing
Published: January 2025

The finite-time cluster synchronization (FTCS) of fractional-order complex-valued (FOCV) neural network has attracted wide attention. It is inconvenient and difficult to decompose complex-valued neural networks into real parts and imaginary parts. This paper addresses the FTCS of coupled memristive neural networks (CMNNs), which are FOCV systems with a time delay. A controller is designed with a complex-valued sign function to achieve FTCS using a non-decomposition approach, which eliminates the need to separate the complex-valued system into its real and imaginary components. By applying fractional-order stability theory, some conditions are derived for FTCS based on the proposed controller. The settling time, related to the system’s initial values, can be computed using the Mittag–Leffler function. We further investigate the optimization of control parameters by formulating an optimization model, which is solved using particle swarm optimization (PSO) to determine the optimal control parameters. Finally, a numerical example and a comparative experiment are both provided to verify the theoretical results and optimization method.
Prospect of (Nd3+) Complexes and Its Nanoparticles as Promising Novel Anticancer Agents in Particular Targeting Breast Cancer Cell Lines
Authors: Abuilaiwi Faraj Ahmad
Published: January 2025

Breast cancer is the leading cause of tumor-related death in women around much of the world and a major health burden for modern medicine. This review highlights the prospect of (Nd3+) complexes and nanoparticles as promising novel anticancer agents in particular targeting breast cancer cell lines. This study emphasizes the therapeutic and diagnostic potentials of Nd3+-based metal complexes, especially in reversing drug resistance or enhancing targeted therapy. A comprehensive overview of diagnostic modalities underscores the imperative for the prompt identification and intervention of breast cancer. Nd3+ complexes demonstrate potential as anticancer therapeutics due to their significant cytotoxicity evidenced by their IC50 values. The research outcomes indicated that it could theoretically inhibit the growth and metastasis of cancer cell lines. Future research should focus on synthesizing novel Nd3+ complexes with enhanced bioavailability, solubility, and reduced toxicity to further advance their application.
The Role of the Immune Response to Helicobacter pylori Antigens and Its Relevance in Gastric Disorders
Authors: Santacroce Luigi , Topi Skender , Cafiero Concetta , Palmirotta Raffaele , Jirillo Emilio
Published: January 2025

Helicobacter pylori (H.p.) is a Gram-negative bacterium endowed with gastric tropism. H.p. infection is widely spread throughout the world, accounting for various pathologies, such as peptic ulcer, gastric cancer, mucosa-associated lymphoid tissue lymphoma, and extra-gastric manifestations. This bacterium possesses several virulence factors, e.g., lipopolysaccharides (LPS), the toxins CagA and VacA, and adhesins, which elicit a robust immune response during the initial phase of the infection. Of note, the lipid A moiety of the LPS exhibits a lower endotoxic potency than that of other LPSs, thus facilitating infection through a mechanism of immune escape. H.p. colonization of the gastric mucosa induces an initial protective immune response with innate immune cells, e.g., neutrophils, monocytes, and macrophages, which engulf and kill bacteria. Moreover, the same cells, along with gastric epithelial cells, secrete cytokines and chemokines, which recruit T cells [T helper (h)1 and Th17 cells] to the site of infection, thus leading to H.p. eradication. In a large subset of individuals, the perturbation of such an immune equilibrium leads to a harmful response, with an expansion of T regulatory (TREG) cells, which suppress the protective immune response. In fact, TREG cells, via the production of interleukin (IL)-10, downregulate Th1- and Th17-related cytokines, thus allowing H.p. survival and the perpetuation of inflammation. As far as the humoral immune response is concerned, B cells, upon H.p. stimulation, produce autoreactive antibodies, and IgG anti-Lex antibodies are harmful to the gastric mucosa. In this review, the structure and function of H.p. antigenic components and immune mechanisms elicited by this bacterium will be described in relation to gastric damage.
Patient Preferences or Provider Pressure? The Relationship Between Coercive Contraceptive Care and Preferred Contraceptive Use
Authors: Swan Laura E. T. , Cannon Lindsay M. , Lands Madison , Zhao Iris Huimeng
Published: January 2025

Background/Objectives: Coercion in contraceptive care occurs when healthcare providers unduly influence patients to use or not use birth control. Contraceptive coercion is antithetical to quality patient-centered care. However, it is unclear how experiencing contraceptive coercion relates to patients’ lives and contraceptive outcomes. In this study, we examined associations between contraceptive coercion and a patient-centered outcome: preferred contraceptive use. Methods: In 2023, we used the Prolific panel to recruit reproductive-aged people in the USA who were assigned female at birth. Our analytic sample included surveyed participants who had ever talked to a healthcare provider about contraception (N = 1197). We conducted chi-square and regression analyses to investigate associations between contraceptive coercion and preferred contraceptive use. We added context by mapping the current and preferred contraceptive method(s) for participants who experienced coercion and were not using their preferred method(s). Results: After adjusting for potential confounders, participants who reported downward coercion (pressure to not use birth control) at their last contraceptive counseling were less likely to be using their preferred contraceptive method(s). The odds of using preferred contraception did not differ significantly based on whether participants experienced upward contraceptive coercion (pressure to use birth control). Patterns in unmet contraceptive preference for patients experiencing coercion include use of the pill when it is not the desired method and unmet desire for permanent contraception. Conclusions: In this study, patients who perceived pressure from a provider to not use birth control were less likely to be using their preferred contraceptive method(s). Promoting reproductive autonomy requires comprehensive, patient-centered, and unbiased contraceptive care.
Refining the Production Date of Historical Palestinian Garments Through Dye Identification
Authors: Tamburini Diego , Durand Ludovic , Klink-Hoppe Zeina
Published: January 2025

The dyes used to produce two Palestinian garments from the British Museum’s collection attributed to the late 19th–early 20th century were investigated by high pressure liquid chromatography coupled with diode array detector and tandem mass spectrometry (HPLC-DAD-MS/MS). Palestinian embroidery is a symbol of national identity and the topic of scholarly research. However, little attention has been given to the dyes and how these changed with the introduction of new synthetic formulations in the second half of the 19th century. The results revealed the use of natural indigoid blue and red madder (Rubia tinctorum), in combination with tannins. Yellow from buckthorn (probably Rhamnus saxatilis) and red from cochineal (probably Dactylopius coccus) were found mixed with synthetic dyes in green and dark red embroidery threads, respectively. Early synthetic dyes were identified in all the other colours. These include Rhodamine B (C.I. 45170), Orange II (C.I. 15510), Orange IV (C.I. 13080), Metanil Yellow (C.I. 13065), Chrysoidine R (C.I. 11320), Methyl Violet (C.I. 42535), Malachite Green (C.I. 42000), Fuchsin (C.I. 42510), Auramine O (C.I. 41000) and Methyl Blue (C.I. 42780). As the date of the first synthesis of these dyes is known, the production date of the garments was refined, suggesting that these were likely to be produced towards the end of the 1880s/beginning of the 1890s. The continuous use of historical local sources of natural dyes, alongside new synthetic dyes, is of particular interest, adding rightful nuances to the development of textile-making practices in this region.
Dynamic Task Planning for Multi-Arm Harvesting Robots Under Multiple Constraints Using Deep Reinforcement Learning
Authors: Xie Feng , Guo Zhengwei , Li Tao , Feng Qingchun , Zhao Chunjiang
Published: January 2025

Global fruit production costs are increasing amid intensified labor shortages, driving heightened interest in robotic harvesting technologies. Although multi-arm coordination in harvesting robots is considered a highly promising solution to this issue, it introduces technical challenges in achieving effective coordination. These challenges include mutual interference among multi-arm mechanical structures, task allocation across multiple arms, and dynamic operating conditions. This imposes higher demands on task coordination for multi-arm harvesting robots, requiring collision-free collaboration, optimization of task sequences, and dynamic re-planning. In this work, we propose a framework that models the task planning problem of multi-arm operation as a Markov game. First, considering multi-arm cooperative movement and picking sequence optimization, we employ a two-agent Markov game framework to model the multi-arm harvesting robot task planning problem. Second, we introduce a self-attention mechanism and a centralized training and execution strategy in the design and training of our deep reinforcement learning (DRL) model, thereby enhancing the model’s adaptability in dynamic and uncertain environments and improving decision accuracy. Finally, we conduct extensive numerical simulations in static environments; when the harvesting targets are set to 25 and 50, the execution time is reduced by 10.7% and 3.1%, respectively, compared to traditional methods. Additionally, in dynamic environments, both operational efficiency and robustness are superior to traditional approaches. The results underscore the potential of our approach to revolutionize multi-arm harvesting robotics by providing a more adaptive and efficient task planning solution. We will research improving the positioning accuracy of fruits in the future, which will make it possible to apply this framework to real robots.
Comparison Between Water Aerobics and Deep-Water Running on Middle-Aged Adults’ Anthropometric, Hemodynamic and Functional Outcomes
Authors: Leonel Larissa dos Santos , Danielevicz Angelica , Delevatti Rodrigo Sudatti
Published: January 2025

Background: Head-out aquatic training, using modalities such as water-aerobics/hydrogymnastics (HYD) and deep-water running (DWR), has been effective in improving the physical, metabolic and cognitive health of middle-aged adults. However, direct comparisons between these modalities are lacking. Aim: The aim of this study was to compare the effects of water aerobics and deep-water running on anthropometric, functional and hemodynamic outcomes in adults and older adults. Methods: An uncontrolled pragmatic trial (RBR-2txw8zy) was conducted with participants aged 30 to 80, allocated to HYD and DWR groups. The intervention consisted of 12 weeks of progressive aerobic training with weekly undulating periodization (2× week), divided into three mesocycles (4, 5, and 3 weeks), each lasting 50 min. Intensity was prescribed using the Rate of Perceived Effort (RPE), ranging from RPE 11 to 17. Outcomes assessed included the 30 s chair stand, 30 s arm curl, Timed-Up-and-Go usual (TUG-u) and maximum (TUG-m), 6 min walking test (6MWT), body mass, waist circumference, blood pressure and resting heart rate-HRrest. The analysis was conducted using generalized estimating equations, with per-protocol (PP) and intention-to-treat (ITT) analyses. Results: The study included 104 participants (HYD: n = 63, mean age 59 years, 54 women; DWR: n = 41, mean age 53 years, 33 women). ITT analysis showed improvements in waist circumference, waist-to-height ratio, and TUG-m in the HYD group, and a reduction in HRrest in the DWR group. Both modalities showed significant improvements in the 30 s chair stand, 30 s arm curl, 6MWT, waist circumference, and waist-to-height ratio in the PP analysis. Conclusions: Both modalities promoted functional improvements and favorable changes in anthropometric evaluations, with DWR showing a greater reduction in HRrest.
Enhancing Accessibility in Public Spaces: A Computational Study of Hatirjheel Lakefront Using Space Syntax
Authors: Hossain Sharif Tousif , Al-Ramadan Baqer , Bilal Muhammad , Altuwaijri Hamad Ahmed
Published: January 2025

Public spaces are vital for urban living, contributing to the environmental, social, and economic aspects of city life. Hatirjheel Lakefront, a newly developed recreational area in Dhaka, offers significant potential for enhancing accessibility and connectivity in a rapidly urbanizing metropolis. This study aims to evaluate global and local integration of access routes and propose strategies to improve pedestrian and vehicular connectivity using Space Syntax methodology and DepthmapX V10 software. The key findings indicate that while Hatirjheel demonstrates strong global integration, regional integration remains moderate, with certain access roads being underutilized due to poor connectivity. Recommendations include enhancing integration through connecting dead-end roads, improving pedestrian pathways, and constructing foot-over bridges to mitigate vehicular traffic barriers. This study contributes to urban planning by providing actionable insights to optimize accessibility in public spaces, supporting recreational and economic activities. The findings are critical for creating a more integrated urban fabric in Dhaka, ensuring sustainable urban growth. This research provides actionable strategies for urban planners to optimize the use of public spaces, reinforcing the role of Hatirjheel as a vital component of Dhaka’s urban network.
Implications of Mineralization Biomarkers in Breast Cancer Outcomes Beyond Calcifications
Authors: Giacobbi Erica , Bonfiglio Rita , Rotondaro Gabriele , Servadei Francesca , Smirnov Artem , Palumbo Valeria , Scioli Maria Paola , Bonanno Elena , Buonomo Claudio Oreste , Vanni Gianluca , Candi Eleonora , Mauriello Alessandro , Scimeca Manuel
Published: January 2025

The aim of this work was to explore the biomarkers associated with epithelial to mesenchymal transition (EMT) and mineralization processes as new prognostic factors across different breast cancer phenotypes. To this end, 133 breast biopsies, including benign and malignant lesions, with or without microcalcifications, were retrospectively collected. Immunohistochemical analysis was performed to evaluate the expression of vimentin, BMP-2, BMP-4, RANKL, Runx2, OPN, PTX3, and SDF-1, while Kaplan—Meier plots were used to assess their prognostic impact on overall survival in a dataset of 2976 breast cancer patients. The expression of vimentin, BMP-2, BMP-4, and SDF-1 was significantly higher in malignant lesions compared to benign ones, regardless of the presence of microcalcifications. Notably, these markers showed no correlation with traditional prognostic factors, such as tumor grade or hormone receptor status. The bioinformatics analysis provided valuable insights into the possible prognostic and therapeutic significance of BMP-2, BMP-4, SDF-1, and vimentin in breast cancer. In fact, all these biomarkers impact on the overall survival in specific molecular breast cancer types. In addition, high expression of SDF-1 and vimentin is able to predict the response to chemotherapy. The findings here reported suggest that vimentin, BMP-2, BMP-4, and SDF-1 could be independent prognostic biomarkers in breast cancer, providing insights beyond traditional clinical factors.
Impact of Genetic Variants on Vitamin E Levels in an Italian Cohort of Bariatric Surgery Patients: A Focus on SNPs Involved with Transport and Bioavailability
Authors: Ricci Claudia , Bufano Annalisa , Iraci Sareri Gabriele , Simon Batzibal Maria , Marzocchi Carlotta , Simoncelli Giorgia , Righi Delia , Salvemini Antonia , Ciuoli Cristina , Di Stefano Leonardo , Benenati Nicoletta , Regoli Tommaso , Miedviedieva Kateryna , Tirone Andrea , Voglino Costantino , Pirisinu Selenia , Cantara Silvia
Published: January 2025

Obesity is a global epidemic associated with chronic inflammation, oxidative stress, and metabolic disorders. Bariatric surgery is a highly effective intervention for sustained weight loss and the improvement of obesity-related comorbidities. However, post-surgery nutritional deficiencies, including vitamin E, remain a concern. This study investigates the role of single-nucleotide polymorphisms (SNPs) in genes related to vitamin E transport and bioavailability in determining vitamin E levels post bariatric surgery. A cohort of 140 patients with obesity undergoing bariatric surgery was analyzed. Serum vitamin E levels were measured before and one year after surgery, and SNPs in genes associated with vitamin E transport and metabolism were genotyped using PCR, DHPLC, and sequencing methods. Associations between SNPs, haplotypes, and vitamin E levels were statistically evaluated. Significant associations were observed between the APOE rs7412 SNP and serum vitamin E levels. The rare T allele was linked to lower vitamin E levels post surgery, with an increased frequency in patients with severe deficiency (<11.6 μmol/L). Haplotype analysis of APOE revealed that the ε2 haplotype (T-T) was strongly associated with vitamin E deficiency. Other SNPs, including CD36 rs1761667, SCARB1 rs4238001, and ABCA1 rs4149314, were also linked to changes in vitamin E levels, suggesting that an impaired bioavailability and transport can be the reason for low vitamin E levels post surgery. Genetic polymorphisms in APOE, CD36, SCARB1, and ABCA1 significantly influence vitamin E status after bariatric surgery. These findings highlight the importance of personalized supplementation strategies considering patients’ genetic profiles to mitigate the risk of vitamin E deficiency and related complications.
Utility of Protein Markers in COVID-19 Patients
Authors: López-Biedma Alicia , Onieva-García María Ángeles , Martín-García Desirée , Redondo Maximino , García-Aranda Marilina
Published: January 2025

COVID-19 has been a challenge at the healthcare level not only in the early stages of the pandemic, but also in the subsequent appearance of long-term COVID-19. Several investigations have attempted to identify proteomic biomarkers in an attempt to improve clinical care, guide treatment and predict possible patient outcomes. Proteins such as C-reactive protein (CRP) or interleukin 6 (IL-6) are clear markers of severe disease, but many others have been proposed that could help in risk stratification and in the prediction of specific complications. This review aims to bring together the most relevant studies in this regard, providing information to identify the most notable biomarkers in relation to COVID-19 found to date.
Application of the OSNA Technique (One-Step Nucleic Acid Amplification Test) in Breast Cancer
Authors: Sychowski Grzegorz , Romanowicz Hanna , Smolarz Beata
Published: January 2025

Breast cancer is one of the most common cancers diagnosed in both countries with high and low levels of socio-academic development. Routine, regular screening tests being introduced in an increasing number of countries make it possible to detect breast cancer at an early stage of development, as a result of which the trend in the incidence of metastatic breast cancer has been decreasing in recent years. The latest guidelines for the treatment of this tumor do not recommend axillary dissection, which limits the need for rapid assessment of the nodes during surgery. Regardless of the progression of the disease, lymph node biopsy and their analysis is one of the most common diagnostic methods for detecting metastases. Systems using one-step amplification of nucleic acids have been present in the diagnosis of breast cancer for nearly 20 years. The one-step nucleic acid amplification (OSNA) test semi-quantitatively detects the number of cytokeratin 19 mRNA copies, a well-known tumor marker, which can be used to infer the presence of metastases in non-sentinel lymph nodes (SLN). Aim: OSNA is a widely used molecular method for SLN, intra-, or postoperative analysis. Its high accuracy has been proved over the years in clinical use. In this review, we checked current state of this technology and compared it to its competitors in the field of breast cancer diagnosis in the era of Axillary Lymph Nodes Dissection (ALND) importance decrease with intention to foresee its further potential use. Objectives: To evaluate OSNA current place in breast cancer diagnosis and treatment we compared OSNA to other lymph node assessing methods. We based our review on original articles and metanalyses published in the last decade. The research was conducted with PubMed, Science Direct, Google Scholar, and NCBI databases. The collected data allowed us to assess the accuracy of OSNA, its cost effectiveness, and its application in other cancers. Results: Regardless of the progression of the disease, a lymph node biopsy and its analysis constitutes one of the most common diagnostic methods for detecting metastases. The OSNA method is characterized by high sensitivity and specificity, and its predictive value has been confirmed by many studies over the years. While its cost effectiveness is still a matter of discussion, this method has been tested more thoroughly than other new lymph nodes assessing technologies. Conclusions: Despite the emergence of competing methods, this test is still widely used as a routine intraoperative examination of lymph nodes. Research carried out in recent years has proved its effectiveness in the diagnosis of other cancers, in the research field, and as a provider of additional data for prognosis improvement.
A Method for the Precise Determination of the Intramolecular Potential Energy Surface on the Basis of Microwave and Submillimeter-Wave Spectra: Diatomic Molecules as a Relevant Illustration
Authors: Ulenikov Oleg , Bekhtereva Elena , Gromova Olga , Sidko Sergei , Bauerecker Sigurd
Published: January 2025

A new method for the precise semiempirical determination of the basic parameters (structural parameters and parameters of the intramolecular potential energy surface, PES) of a molecule on the basis of highly accurate experimental data from the microwave and submillimeter-wave regions is suggested. The options and advantages of this method in comparison with the other methods of molecular PES determination are discussed using a diatomic molecule as an appropriate illustration. The HCl molecule is exploited as a suitable example. It is shown with this example that the use of a very limited number (ten for H35Cl and five for D35Cl) of submillimeter-wave line positions allows one to determine the values of the equilibrium rotational parameter, harmonic frequency, and anharmonic coefficients of the third, fourth, and fifth order with accuracy of 0.01%, 0.01%, 0.01%, 2.1%, and 10.1%, respectively, in comparison with the analogous results obtained from extensive infrared studies.
Evaluation of Hypoxia Markers in Critically Ill Patients Categorized by Their Burden of Organ Dysfunction: A Novel Approach to Detect Pathophysiological and Clinical Relevance in a Secondary Analysis of a Prospective Observational Study
Authors: Centner Franz-Simon , Brohm Kathrin , Mindt Sonani , Jaeger Evelyn , Hahn Bianka , Fuderer Tanja , Lindner Holger A. , Schneider-Lindner Verena , Krebs Joerg , Neumaier Michael , Thiel Manfred , Schoettler Jochen J.
Published: January 2025

In critically ill patients, compromised microcirculation causes tissue hypoxia, organ failure, and death. These pathophysiological processes occur particularly in patients with high illness severity, so reliable hypoxia biomarkers should reflect this in their occurrence. This secondary analysis of a prospective study categorized patients by their burden of organ dysfunction (BOD) using the cohort’s median initial sequential organ failure assessment (SOFA) score of 8 as a cutoff. The kinetic parameters of the hypoxia markers lactate and S-adenosylhomocysteine (SAH) were analyzed for correlation with organ dysfunction severity and mortality prediction. In low BOD patients, neither marker correlated with SOFA. In high BOD patients, lactate showed a moderate correlation and SAH showed a strong correlation. Lactate correlated with organ dysfunction in survivors but not in non-survivors, while SAH correlated strongly in non-survivors but not in survivors. In univariate logistic regression, lactate predicted mortality moderately in low BOD (areas under the receiver operating characteristic curves (AUROCs) 0.7–0.8) but poorly in high BOD patients (AUROCs 0.5–0.7). SAH’s prediction improved from poor to excellent (AUROCs 0.8–0.9) with higher BOD. Thus, SAH appears superior to lactate in the detection of organ dysfunction severity and mortality prediction in high BOD patients.
Polymorphisms Influence the Expression of the Fas and FasL Genes in COVID-19
Authors: dos Santos Brito Wandrey Roberto , de Brito William Botelho , dos Santos Ferreira Fabiane , Santana Emmanuelle Giuliana Mendes , da Costa Lopes Jeferson , da Silva Graça Amoras Ednelza , Lima Sandra Souza , dos Santos Erika Ferreira , da Costa Flávia Póvoa , de Sarges Kevin Matheus Lima , Cantanhede Marcos Henrique Damasceno , de Brito Mioni Thieli Figueiredo Magalhães , da Silva Andréa Luciana Soares , de Meira Leite Mauro , de Nazaré do Socorro de Almeida Viana Maria , Rodrigues Fabíola Brasil Barbosa , da Silva Rosilene , Viana Giselle Maria Rachid , do Socorro Souza Chaves Tânia , de Oliveira Lameira Veríssimo Adriana , da Silva Carvalho Mayara , Henriques Daniele Freitas , da Silva Carla Pinheiro , Nunes Juliana Abreu Lima , Costa Iran Barros , Brasil-Costa Igor , Quaresma Juarez Antônio Simões , Cayres-Vallinoto Izaura Maria Vieira , Reis Leonardo Oliveira , Falcão Luiz Fábio Magno , dos Santos Eduardo José Melo , Vallinoto Antonio Carlos Rosário , Queiroz Maria Alice Freitas
Published: January 2025

The apoptotic molecule Fas and its ligand FasL are involved in the process of T-lymphocyte death, which may lead to lymphopenia, a characteristic of severe coronavirus disease 2019 (COVID-19). In this study, we investigated the influence of polymorphisms in the FAS and FASL genes, FAS and FASL gene expression, and plasma cytokine levels on COVID-19 severity and long COVID occurrence. A total of 116 individuals with severe COVID-19 and 254 with the non-severe form of the disease were evaluated. In the post-COVID-19 period, samples from 196 individuals with long COVID and 67 from people who did not have long COVID were included. Genotyping and quantification of gene expression were performed via real-time PCR, and cytokine measurement was performed via flow cytometry. The AA genotype for FAS rs1800682 (A/G) and the TT genotype for FASL rs763110 (C/T) were associated with increased FAS and FASL gene expression, respectively (p < 0.005). Higher plasma IFN-γ levels were associated with higher FAS and FASL gene expression (p < 0.05). Among individuals with non-severe COVID-19, carriers of the AA genotype for FAS rs1800682 (A/G) had higher levels of FAS expression, more symptoms, and higher IFN-γ levels (p < 0.05). No association of the evaluated markers with long COVID were observed. The AA genotype of FAS rs1800682 (A/G) and the TT genotype of FASL rs763110 (C/T) influence the levels of FAS and FASL gene expression. Higher gene expression of FAS and FASL may lead to greater inflammation in COVID-19 patients, with higher levels of IFN-γ and T lymphocyte death.
Use of Light-Emitting Diodes on the In Vitro Rooting of Apple Tree Rootstocks
Authors: Tomazini Scolaro Adriana Maria , De Martin Mariuccia Schlichting , Vieira Renato Luis , Schveitzer Bianca , de Souza Edson Luiz , Borges Endler Marcel
Published: January 2025

This study presents a pioneering investigation into the use of Light Emitting Diodes (LEDs) for in vitro rooting of ‘Marubakaido’ apple tree rootstocks, marking the first report of this approach in the literature. The research evaluates the effects of four distinct light sources: blue LED (450 nm), red LED (660 nm), a combination of red and blue LEDs, and traditional fluorescent lamps as a control. Mini-cuttings were inoculated in Murashige and Skoog (MS) medium with reduced nutrient concentrations, supplemented with indoleacetic acid (IAA) and sucrose. The explants were incubated under controlled conditions for 30 days, enabling a comprehensive assessment of the impact of different light sources on various growth metrics. The results revealed that blue LEDs significantly enhanced dry mass accumulation in seedlings compared to both red LEDs and fluorescent lamps, demonstrating their superior effectiveness in promoting plant growth. The use of LEDs not only improves seedling development but also offers economic advantages over fluorescent lamps. LEDs are characterized by high luminous efficiency, low energy consumption, and a long operational lifespan, which collectively reduce costs in plant production systems. This research advances the understanding of light-mediated effects on plant tissue culture and highlights the potential of combining blue and red LEDs as a viable alternative to fluorescent lighting. These findings could revolutionize practices in horticulture and plant propagation, providing a more efficient and sustainable approach to in vitro cultivation.
Analysis of Biomolecular Changes in HeLa Cervical Cancer Cell Line Induced by Interaction with [Pd(dach)Cl2]
Authors: Ralić Vanja , Nešić Maja D. , Dučić Tanja , Stepić Milutin , Korićanac Lela , Davalieva Katarina , Petković Marijana
Published: January 2025

Transition metal complexes have been used in medicine for several decades, but their intracellular effects are not yet fully elucidated. Therefore, in this study, we investigate biomolecular changes induced by a palladium(II) complex in cervical carcinoma (HeLa) cells as a model to study the subtle changes caused by transition metal ions ingested by the cells. The impact of dichloro(1,2-diaminocyclohexane)palladium(II), [Pd(dach)Cl2], was studied by synchrotron radiation-based Fourier transform infrared (SR FTIR) spectroscopy, a powerful tool for studying alterations in cellular components’ biochemical composition and biomolecular secondary structure on a single-cell level. A spectral analysis, complemented by statistics, revealed that the Pd(II) complex considerably affected all major types of macromolecules in HeLa cells and induced structural changes in proteins through an increased formation of cross-β-sheets and causes structural rearrangement in deoxyribonucleic acid (DNA) through potential chromosome fragmentation. Although a certain level of lipid peroxidation was detectable by SR FTIR spectroscopy and confirmed by an analysis of cellular lipids by matrix-assisted laser desorption and ionisation time-of-flight mass spectrometry, the oxidative stress is not a significant mechanism by which Pd(II) expresses the effect on the HeLa cells.
Evaluating the Impact of Climate Change on the Asia Habitat Suitability of Troides helena Using the MaxEnt Model
Authors: Yang Fengrong , Liu Quanwei , Yang Junyi , Liu Biyu , Deng Xinqi , Gan Tingjiang , Liao Xue , Li Xiushan , Xu Danping , Zhuo Zhihang
Published: January 2025

Butterflies are highly sensitive to climate change, and Troides helena, as an endangered butterfly species, is also affected by these changes. To enhance the conservation of T. helena and effectively plan its protected areas, it is crucial to understand the potential impacts of climate change on its distribution. This study utilized a MaxEnt model in combination with ArcGIS technology to predict the global potential suitable habitats of T. helena under current and future climate conditions, using the species’ distribution data and relevant environmental variables. The results indicated that the MaxEnt model provided a good prediction accuracy for the distribution of T. helena. Under the current climate scenario, the species is primarily distributed in tropical regions, with high suitability areas concentrated in tropical rainforest climates. In future climate scenarios, the suitable habitat areas for T. helena in medium and high suitability categories generally show an expansion trend, which increases over time. Especially under the SSP5-8.5 scenario, by the 2090s, the area of high suitability for T. helena is projected to increase by 42.85%. The analysis of key environmental factors revealed that precipitation of the wettest quarter (Bio16) was the most significant environmental factor affecting the distribution of T. helena. The species has high demands for precipitation and temperature and can adapt to future climate warming. This study is valuable for identifying the optimal conservation areas for T. helena and provides a reference for future conservation efforts.
Analysis of Calcium Patterns in the Thoracic Aorta and Clinical Outcomes of TAVR Patients Presenting with Porcelain Aorta
Authors: Campanella Caterina , Voss Stephanie , Schreyer Julia , Puluca Nazan , Amabile Andrea , Wirth Felix , Krane Markus , Ruge Hendrik
Published: January 2025

Background/Objectives: In the presence of porcelain aorta (PA), transcatheter aortic valve replacement (TAVR) has become a class I therapeutic indication for the treatment of severe aortic valve stenosis. To date, few studies have analyzed the clinical outcomes of TAVR in PA patients. We aim to analyze the calcification patterns of the thoracic aorta in PA patients and to evaluate their clinical implications for TAVR procedures. Methods: This study included 161 patients who had PA confirmed through pre-operative CT and underwent TAVR between 11/2014 and 12/2022. The primary outcome was to perform a multi-slice CT (MSCT) analysis assessing the calcification in the proximal, middle, and distal thoracic aortic segments. Each segment was divided into quadrants for scoring calcifications on a scale from 1 (<25%) to 4 (>75%). The cohort was categorized into circular or noncircular calcification group. The secondary clinical outcomes were defined according to VARC-3 criteria. Results: The study cohort included 161 patients (median age, 77.2 years; IQR, 70.1–82.6 years). The median EuroSCORE II and STS predicted risk of mortality were 3.10% [1.80–5.58] and 2.70% [1.70–4.30], respectively. In 75% of patients (n = 121/161), TAVR was performed via transfemoral access. Circular calcifications were found in 8.1% of patients, while noncircular calcifications were present in 91.9%. Significant calcifications were primarily in the right quadrant of the proximal segment (33.54%), superior quadrant of the middle segment (39.75%), and left quadrant of the distal segment (73.29%). The 30-day mortality rate was 3.11% and periprocedural ischemic stroke rate 3.38%. Conclusions: Most TAVR patients with PA exhibited noncircular calcification. The most extensive calcifications were primarily in areas relevant to surgical manipulation. Patients with PA displayed low short-term mortality and relatively few stroke events. In view of these findings, TAVR constitutes a valid treatment option for patients with PA and aortic stenosis.