bluuebunny/crossref_metadata_2025 huggingface dataset:
Application of Passive Sampling to Characterise the Fish Exometabolome
Authors: Viant Mark , Elphinstone Davis Jessica , Duffy Cathleen , Engel Jasper , Stenton Craig , Sebire Marion , Katsiadaki Ioanna
Published: February 2017

The endogenous metabolites excreted by organisms into their surrounding environment, termed the exometabolome, are important for many processes including chemical communication. In fish biology, such metabolites are also known to be informative markers of physiological status. While metabolomics is increasingly used to investigate the endogenous biochemistry of organisms, no non-targeted studies of the metabolic complexity of fish exometabolomes have been reported to date. In environmental chemistry, Chemcatcher® (Portsmouth, UK) passive samplers have been developed to sample for micro-pollutants in water. Given the importance of the fish exometabolome, we sought to evaluate the capability of Chemcatcher® samplers to capture a broad spectrum of endogenous metabolites excreted by fish and to measure these using non-targeted direct infusion mass spectrometry metabolomics. The capabilities of C18 and styrene divinylbenzene reversed-phase sulfonated (SDB-RPS) Empore™ disks for capturing non-polar and polar metabolites, respectively, were compared. Furthermore, we investigated real, complex metabolite mixtures excreted from two model fish species, rainbow trout (Oncorhynchus mykiss) and three-spined stickleback (Gasterosteus aculeatus). In total, 344 biological samples and 28 QC samples were analysed, revealing 646 and 215 m/z peaks from trout and stickleback, respectively. The measured exometabolomes were principally affected by the type of Empore™ (Hemel Hempstead, UK) disk and also by the sampling time. Many peaks were putatively annotated, including several bile acids (e.g., chenodeoxycholate, taurocholate, glycocholate, glycolithocholate, glycochenodeoxycholate, glycodeoxycholate). Collectively these observations show the ability of Chemcatcher® passive samplers to capture endogenous metabolites excreted from fish.
Micro-Hole Drilling on Glass Substrates—A Review
Authors: Hof Lucas , Abou Ziki Jana
Published: February 2017

Glass micromachining is currently becoming essential for the fabrication of micro-devices, including micro- optical-electro-mechanical-systems (MOEMS), miniaturized total analysis systems (μTAS) and microfluidic devices for biosensing. Moreover, glass is radio frequency (RF) transparent, making it an excellent material for sensor and energy transmission devices. Advancements are constantly being made in this field, yet machining smooth through-glass vias (TGVs) with high aspect ratio remains challenging due to poor glass machinability. As TGVs are required for several micro-devices, intensive research is being carried out on numerous glass micromachining technologies. This paper reviews established and emerging technologies for glass micro-hole drilling, describing their principles of operation and characteristics, and their advantages and disadvantages. These technologies are sorted into four machining categories: mechanical, thermal, chemical, and hybrid machining (which combines several machining methods). Achieved features by these methods are summarized in a table and presented in two graphs. We believe that this paper will be a valuable resource for researchers working in the field of glass micromachining as it provides a comprehensive review of the different glass micromachining technologies. It will be a useful guide for advancing these techniques and establishing new hybrid ones, especially since this is the first broad review in this field.
Droplet Dynamics of Newtonian and Inelastic Non-Newtonian Fluids in Conﬁnement
Authors: Ioannou Nikolaos , Liu Haihu , Oliveira Mónica , Zhang Yonghao
Published: February 2017

Microﬂuidic droplet technology has been developing rapidly. However, precise control of dynamical behaviour of droplets remains a major hurdle for new designs. This study is to understand droplet deformation and breakup under simple shear ﬂow in conﬁned environment as typically found in microﬂuidic applications. In addition to the Newtonian–Newtonian system, we consider also both a Newtonian droplet in a non-Newtonian matrix ﬂuid and a non-Newtonian droplet in a Newtonian matrix. The lattice Boltzmann method is adopted to systematically investigate droplet deformation and breakup under a broad range of capillary numbers, viscosity ratios of the ﬂuids, and conﬁnement ratios considering shear-thinning and shear-thickening ﬂuids. Conﬁnement is found to enhance deformation, and the maximum deformation occurs at the viscosity ratio of unity. The droplet orients more towards the ﬂow direction with increasing viscosity ratio or conﬁnement ratio. In addition, it is noticed that the wall effect becomes more signiﬁcant for conﬁnement ratios larger than 0.4. Finally, for the whole range of Newtonian carrier ﬂuids tested, the critical capillary number above which droplet breakup occurs is only slightly affected by the conﬁnement ratio for a viscosity ratio of unity. Upon increasing the conﬁnement ratio, the critical capillary number increases for the viscosity ratios less than unity, but decreases for the viscosity ratios more than unity.
Dissolution and Sorption Processes on the Surface of Calcite in the Presence of High Co2+ Concentration
Authors: González-López Jorge , Fernández-González Ángeles , Jiménez Amalia , Godelitsas Athanasios , Ladas Spyridon , Provatas Georgios , Lagogiannis Anastasios , Pasias Ioannis , Thomaidis Nikolaos , Prieto Manuel
Published: February 2017

The interaction of the calcite surface with Co2+-rich aqueous solutions ([Co2+aq]initial = 1000 ppm, i.e., ca. 17 mM) was investigated by means of macroscopic experiments and surface spectroscopic techniques. In the case of the macroscopic experiments, calcite powder and monocrystals were immersed into solutions for different time periods (from 1 min to one month). The Ca concentrations in the filtrates was measured by means of atomic absorption spectrometry (AAS) while the interacted solids were studied using a combination of X-ray photoelectron spectroscopy (XPS) and 12C-rutherford backscattering spectrometry (12C-RBS). The macroscopic data showed a characteristic surface dissolution process, in parallel to the surface sorption processes. Adsorption and co-precipitation were seen for almost the entire immersion period for both calcite powder and monocrystals. The surface study by XPS (analyzed at a depth of approximately 12 nm) suggested that adsorption takes place in the first hour of the interaction, followed by incorporation of Co2+ into calcite surface layers, leading to the formation of a Co2+-bearing surface (co)precipitate, which occurs over a period of hours and days. The 12C-RBS measurements on calcite { 10 1 ¯ 4 } indicated that the thickness of this surface co-precipitate was 270 nm after one day and then stabilized at 320 nm after more than a week.
The Architecture of Thiol Antioxidant Systems among Invertebrate Parasites
Authors: Guevara-Flores Alberto , Martínez-González José , Rendón Juan , Del Arenal Irene
Published: February 2017

The use of oxygen as the final electron acceptor in aerobic organisms results in an improvement in the energy metabolism. However, as a byproduct of the aerobic metabolism, reactive oxygen species are produced, leaving to the potential risk of an oxidative stress. To contend with such harmful compounds, living organisms have evolved antioxidant strategies. In this sense, the thiol-dependent antioxidant defense systems play a central role. In all cases, cysteine constitutes the major building block on which such systems are constructed, being present in redox substrates such as glutathione, thioredoxin, and trypanothione, as well as at the catalytic site of a variety of reductases and peroxidases. In some cases, the related selenocysteine was incorporated at selected proteins. In invertebrate parasites, antioxidant systems have evolved in a diversity of both substrates and enzymes, representing a potential area in the design of anti-parasite strategies. The present review focus on the organization of the thiol-based antioxidant systems in invertebrate parasites. Differences between these taxa and its final mammal host is stressed. An understanding of the antioxidant defense mechanisms in this kind of parasites, as well as their interactions with the specific host is crucial in the design of drugs targeting these organisms.
Anticancer Activities of C18-, C19-, C20-, and Bis-Diterpenoid Alkaloids Derived from Genus Aconitum
Authors: Ren Meng-Yue , Yu Qing-Tian , Shi Chun-Yu , Luo Jia-Bo
Published: February 2017

Cancer is one of the most common lethal diseases, and natural products have been extensively studied as anticancer agents considering their availability, low toxicity, and economic affordability. Plants belonging to the genus Aconitum have been widely used medically in many Asian countries since ancient times. These plants have been proven effective for treating several types of cancer, such as lung, stomach, and liver cancers. The main effective components of Aconitum plants are diterpenoid alkaloids—which are divided into C18-, C19-, C20-, and bis-diterpenoid alkaloids—are reportedly some of the most promising, naturally abundant compounds for treating cancer. This review focuses on the progress of diterpenoid alkaloids with different structures derived from Aconitum plants and some of their derivatives with potential anticancer activities. We hope that this work can serve as a reference for further developing Aconitum diterpenoid alkaloids as anticancer agents.
A Protein Isolate from Moringa oleifera Leaves Has Hypoglycemic and Antioxidant Effects in Alloxan-Induced Diabetic Mice
Authors: Paula Paulo , Sousa Daniele , Oliveira Jose , Carvalho Ana , Alves Bella , Pereira Mirella , Farias Davi , Viana Martonio , Santos Flavia , Morais Talita , Vasconcelos Ilka
Published: February 2017

Moringa oleifera has been used in traditional medicine to treat diabetes. However, few studies have been conducted to relate its antidiabetic properties to proteins. In this study, a leaf protein isolate was obtained from M. oleifera leaves, named Mo-LPI, and the hypoglycemic and antioxidant effects on alloxan-induced diabetic mice were assessed. Mo-LPI was obtained by aqueous extraction, ammonium sulphate precipitation and dialysis. The electrophoresis profile and proteolytic hydrolysis confirmed its protein nature. Mo-LPI showed hemagglutinating activity, cross-reaction with anti-insulin antibodies and precipitation after zinc addition. Single-dose intraperitoneal (i.p.) administration of Mo-LPI (500 mg/kg·bw) reduced the blood glucose level (reductions of 34.3%, 60.9% and 66.4% after 1, 3 and 5 h, respectively). The effect of Mo-LPI was also evidenced in the repeated dose test with a 56.2% reduction in the blood glucose level on the 7th day after i.p. administration. Mo-LPI did not stimulate insulin secretion in diabetic mice. Mo-LPI was also effective in reducing the oxidative stress in diabetic mice by a decrease in malondialdehyde level and increase in catalase activity. Mo-LPI (2500 mg/kg·bw) did not cause acute toxicity to mice. Mo-LPI is a promising alternative or complementary agent to treat diabetes.
Anti-Bacterial and Anti-Fungal Activity of Xanthones Obtained via Semi-Synthetic Modification of α-Mangostin from Garcinia mangostana
Authors: Narasimhan Srinivasan , Maheshwaran Shanmugam , Abu-Yousef Imad , Majdalawieh Amin , Rethavathi Janarthanam , Das Prince , Poltronieri Palmiro
Published: February 2017

The microbial contamination in food packaging has been a major concern that has paved the way to search for novel, natural anti-microbial agents, such as modified α-mangostin. In the present study, twelve synthetic analogs were obtained through semi-synthetic modification of α-mangostin by Ritter reaction, reduction by palladium-carbon (Pd-C), alkylation, and acetylation. The evaluation of the anti-microbial potential of the synthetic analogs showed higher bactericidal activity than the parent molecule. The anti-microbial studies proved that I E showed high anti-bacterial activity whereas I I showed the highest anti-fungal activity. Due to their microbicidal potential, modified α-mangostin derivatives could be utilized as active anti-microbial agents in materials for the biomedical and food industry.
Resveratrol and Grape Extract-loaded Solid Lipid Nanoparticles for the Treatment of Alzheimer’s Disease
Authors: Loureiro Joana , Andrade Stephanie , Duarte Ana , Neves Ana , Queiroz Joana , Nunes Cláudia , Sevin Emmanuel , Fenart Laurence , Gosselet Fabien , Coelho Manuel , Pereira Maria
Published: February 2017

The aggregation of amyloid-β peptide (Aβ) has been linked to the formation of neuritic plaques, which are pathological hallmarks of Alzheimer’s disease (AD). Various natural compounds have been suggested as therapeutics for AD. Among these compounds, resveratrol has aroused great interest due to its neuroprotective characteristics. Here, we provide evidence that grape skin and grape seed extracts increase the inhibition effect on Aβ aggregation. However, after intravenous injection, resveratrol is rapidly metabolized into both glucuronic acid and sulfate conjugations of the phenolic groups in the liver and intestinal epithelial cells (within less than 2 h), which are then eliminated. In the present study, we show that solid lipid nanoparticles (SLNs) functionalized with an antibody, the anti-transferrin receptor monoclonal antibody (OX26 mAb), can work as a possible carrier to transport the extract to target the brain. Experiments on human brain-like endothelial cells show that the cellular uptake of the OX26 SLNs is substantially more efficient than that of normal SLNs and SLNs functionalized with an unspecific antibody. As a consequence, the transcytosis ability of these different SLNs is higher when functionalized with OX-26.
High-Yield Production of Levulinic Acid from Pretreated Cow Dung in Dilute Acid Aqueous Solution
Authors: Su Jialei , Shen Feng , Qiu Mo , Qi Xinhua
Published: February 2017

Agricultural waste cow dung was used as feedstock for the production of a high value–added chemical levulinic acid (LA) in dilute acid aqueous solutions. A high LA yield of 338.9 g/kg was obtained from the pretreated cow dung, which was much higher than that obtained from the crude cow dung (135 g/kg), mainly attributed to the breakage of the lignin fraction in the lignocellulose structure of the cow dung by potassium hydroxide (KOH) pretreatment, and thus enhanced the accessibility of cow dung to the acid sites in the catalytic reaction. Meanwhile, another value-added chemical formic acid could be obtained with a yield of ca. 160 g/kg in the process, implying a total production of ca. 500 g/kg yield for LA and formic acid from the pretreated cow dung with the proposed process. The developed process was shown to be tolerant to high initial substrate loading with a satisfied LA yield. This work provides a promising strategy for the value-increment utilization of liglocellulosic agricultural residues.
Wine Flavonoids in Health and Disease Prevention
Authors: Fernandes Iva , Pérez-Gregorio Rosa , Soares Susana , Mateus Nuno , De Freitas Victor
Published: February 2017

Wine, and particularly red wine, is a beverage with a great chemical complexity that is in continuous evolution. Chemically, wine is a hydroalcoholic solution (~78% water) that comprises a wide variety of chemical components, including aldehydes, esters, ketones, lipids, minerals, organic acids, phenolics, soluble proteins, sugars and vitamins. Flavonoids constitute a major group of polyphenolic compounds which are directly associated with the organoleptic and health-promoting properties of red wine. However, due to the insufficient epidemiological and in vivo evidences on this subject, the presence of a high number of variables such as human age, metabolism, the presence of alcohol, the complex wine chemistry, and the wide array of in vivo biological effects of these compounds suggest that only cautious conclusions may be drawn from studies focusing on the direct effect of wine and any specific health issue. Nevertheless, there are several reports on the health protective properties of wine phenolics for several diseases such as cardiovascular diseases, some cancers, obesity, neurodegenerative diseases, diabetes, allergies and osteoporosis. The different interactions that wine flavonoids may have with key biological targets are crucial for some of these health-promoting effects. The interaction between some wine flavonoids and some specific enzymes are one example. The way wine flavonoids may be absorbed and metabolized could interfere with their bioavailability and therefore in their health-promoting effect. Hence, some reports have focused on flavonoids absorption, metabolism, microbiota effect and overall on flavonoids bioavailability. This review summarizes some of these major issues which are directly related to the potential health-promoting effects of wine flavonoids. Reports related to flavonoids and health highlight some relevant scientific information. However, there is still a gap between the knowledge of wine flavonoids bioavailability and their health-promoting effects. More in vivo results as well as studies focused on flavonoid metabolites are still required. Moreover, it is also necessary to better understand how biological interactions (with microbiota and cells, enzymes or general biological systems) could interfere with flavonoid bioavailability.
An Overview of Stress-Induced Resveratrol Synthesis in Grapes: Perspectives for Resveratrol-Enriched Grape Products
Authors: Hasan Mohidul , Bae Hanhong
Published: February 2017

Resveratrol is the most important stilbene phytoalexin synthesized naturally or induced in plants, as a part of their defense mechanism. Grapes and their derivative products, including juice and wine, are the most important natural sources of resveratrol, consisting of notably higher amounts than other natural sources like peanuts. Consumption of red wine with its presence of resveratrol explained the “French Paradox”. Hence, the demand of resveratrol from grapes is increasing. Moreover, as a natural source of resveratrol, grapes became very important in the nutraceutical industry for their benefits to human health. The accumulation of resveratrol in grape skin, juice, and wine has been found to be induced by the external stimuli: microbial infection, ultrasonication (US) treatment, light-emitting diode (LED), ultra violet (UV) irradiation, elicitors or signaling compounds, macronutrients, and fungicides. Phenylalanine ammonia lyase, cinnamate-4-hydroxylase, coumaroyl-CoA ligase, and stilbene synthase play a key role in the synthesis of resveratrol. The up-regulation of those genes have the positive relationship with the elicited accumulation of resveratrol. In this review, we encapsulate the effect of different external stimuli (biotic and abiotic stresses or signaling compounds) in order to obtain the maximum accumulation of resveratrol in grape skin, leaves, juice, wine, and cell cultures.
CirComPara: A Multi‐Method Comparative Bioinformatics Pipeline to Detect and Study circRNAs from RNA‐seq Data
Authors: Gaffo Enrico , Bonizzato Annagiulia , Kronnie Geertruy , Bortoluzzi Stefania
Published: February 2017

Circular RNAs (circRNAs) are generated by backsplicing of immature RNA forming covalently closed loops of intron/exon RNA molecules. Pervasiveness, evolutionary conservation, massive and regulated expression, and posttranscriptional regulatory roles of circRNAs in eukaryotes have been appreciated and described only recently. Moreover, being easily detectable disease markers, circRNAs undoubtedly represent a molecular class with high bearing on molecular pathobiology. CircRNAs can be detected from RNAseq data using appropriate computational methods to identify the sequence reads spanning backsplice junctions that do not colinearly map to the reference genome. To this end, several programs were developed and critical assessment of various strategies and tools suggested the combination of at least two methods as good practice to guarantee robust circRNA detection. Here,we present CirComPara (http://github.com/egaffo/CirComPara), an automated bioinformatics pipeline, to detect, quantify and annotate circRNAs from RNAseq data using in parallel four different methods for backsplice identification. CirComPara also provides quantification of linear RNAs and gene expression, ultimately comparing and correlating circRNA and gene/transcript expression level. We applied our method to RNAseqdata of monocyte and macrophage samples in relation to haploinsufficiency of the RNAbinding splicing factor Quaking (QKI). The biological relevance of the results, in terms of number, types and variations of circRNAs expressed, illustrates CirComPara potential to enlarge the knowledge of the transcriptome, adding details on the circRNAome, and facilitating further computational and experimental studies.
Dietary Fiber and the Human Gut Microbiota: Application of Evidence Mapping Methodology
Authors: Sawicki Caleigh , Livingston Kara , Obin Martin , Roberts Susan , Chung Mei , McKeown Nicola
Published: February 2017

Interest is rapidly growing around the role of the human gut microbiota in facilitating beneficial health effects associated with consumption of dietary fiber. An evidence map of current research activity in this area was created using a newly developed database of dietary fiber intervention studies in humans to identify studies with the following broad outcomes: (1) modulation of colonic microflora; and/or (2) colonic fermentation/short-chain fatty acid concentration. Study design characteristics, fiber exposures, and outcome categories were summarized. A sub-analysis described oligosaccharides and bacterial composition in greater detail. One hundred eighty-eight relevant studies were identified. The fiber categories represented by the most studies were oligosaccharides (20%), resistant starch (16%), and chemically synthesized fibers (15%). Short-chain fatty acid concentration (47%) and bacterial composition (88%) were the most frequently studied outcomes. Whole-diet interventions, measures of bacterial activity, and studies in metabolically at-risk subjects were identified as potential gaps in the evidence. This evidence map efficiently captured the variability in characteristics of expanding research on dietary fiber, gut microbiota, and physiological health benefits, and identified areas that may benefit from further research. We hope that this evidence map will provide a resource for researchers to direct new intervention studies and meta-analyses.
Dietary Fatty Acids and Changes in Blood Lipids during Adolescence: The Role of Substituting Nutrient Intakes
Authors: Harris Carla , Buyken Anette , Koletzko Sibylle , Von Berg Andrea , Berdel Dietrich , Schikowski Tamara , Koletzko Berthold , Heinrich Joachim , Standl Marie
Published: February 2017

The relevance of dietary fatty acids (FA) for blood lipids should be assessed in the context of substituting nutrients. Such evidence is lacking for adolescents. This study describes prospective associations of dietary FA with changes in serum lipids during adolescence, and considers the theoretical isocaloric replacements of saturated FA (SFA) with other FA or carbohydrates (CHO). Children from the GINIplus and LISAplus birth cohorts, with data on FA intakes (at age 10 years) and serum lipids (at age 10 and 15 years), were included (n = 1398). Associations of SFA, monounsaturated FA (MUFA), n-3 polyunsaturated FA (n-3 PUFA) and n-6 PUFA, with changes in low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglycerides (TAG), and total cholesterol to HDL ratio (TOTAL:HDL), were assessed by linear regression. Substitution models assessed isocaloric replacements of SFA with MUFA, n-3 PUFA, n-6 PUFA or CHO. Higher SFA intakes were associated with decreasing TAG. No associations were observed for fatty acid intakes with LDL, HDL or TOTAL:HDL. In females, replacing SFA with CHO was associated with increasing LDL, TAG and TOTAL:HDL. Our findings confirm observations in adults, although sex-specific determinants seem relevant in our adolescent population. Overlooking the nutrient context when limiting SFA intakes might have detrimental consequences appreciable as early as adolescence.
Validity of the Food Frequency Questionnaire Assessing the Folate Intake in Women of Reproductive Age Living in a Country without Food Fortification: Application of the Method of Triads
Authors: Zekovic Milica , Djekic-Ivankovic Marija , Nikolic Marina , Gurinovic Mirjana , Krajnovic Dusanka , Glibetic Marija
Published: February 2017

The study aimed to examine the external validity of the Folate Food Frequency Questionnaire (F-FFQ) designed for assessing the folate intake in Serbian women of reproductive age. The F-FFQ was tested against repeated 24 h dietary recalls and correspondent nutritional biomarkers (red blood cells (RBC) and serum folate concentrations) using the method of triads. In a cross sectional study, 503 women aged 18–49 years completed dietary questionnaires and representative validation subsample (n = 50) provided fasting blood samples for biomarker analyses. Correlation coefficients were calculated between each of the dietary methods and three pair-wise correlations were applied for the calculation of validity coefficients. Correlation coefficients observed between F-FFQ and three 24 h recalls were r = 0.56 (p < 0.001) and r = 0.57 (p < 0.001) for total sample and validation group, respectively. Bland–Altman plot and cross-classification analyses indicated good agreement between methods. High validity coefficients were determined between the true intake (I) and dietary assessment methods, F-FFQ (Q) and 24 h dietary recalls (R) (ρQIrbc = 0.871 and ρQIser = 0.814; ρRIrbc = 0.652 and ρRIser = 0.698), and moderate ones for biomarkers (B) (ρBIrbc = 0.428 and ρBIser = 0.421). The F-FFQ is valid instrument for the assessment of dietary folate intake in women living in Serbia, a country without mandatory folic acid food fortification.
Adequacy of Usual Vitamin and Mineral Intake in Spanish Children and Adolescents: ENALIA Study
Authors: López-Sobaler Ana , Aparicio Aránzazu , González-Rodríguez Liliana , Cuadrado-Soto Esther , Rubio Josefa , Marcos Victoria , Sanchidrián Rosa , Santos Sara , Pérez-Farinós Napoleón , Dal Re Marian , Villar Carmen , Robledo Teresa , Castrodeza J. , Ortega Rosa
Published: February 2017

Background: The National Dietary Survey on the Child and Adolescent Population in Spain (ENALIA) provides data to assess the usual micronutrient intake among Spanish infants, children, and adolescents. Methods: Cross-sectional survey (November 2012–July 2014) of a representative sample of Spanish children and adolescents (six months–17 years) (n = 1862). Dietary information was collected using two non-consecutive one-day food diaries (six months–10 years old) or two 24 h dietary recalls (11 years and older) separated by at least 14 days. Estimates were calculated using the Iowa State University method and PC-SIDE software (version 1.0, department of statistics, center for agricultural and rural development, Ames, IA, USA) to account for within- and between-person variation. Results: Usual intake of vitamin D was insufficient in practically all individuals. Vitamin E, folate, and calcium were insufficient, especially from nine years of age, and magnesium and iodine from 14 years of age. The percentage of subjects with insufficient intakes was higher among females. Sodium intake was excessive in a considerable percentage of the population, especially in males, and it increased with age. Finally, over half of children under four years of age had zinc usual intakes that exceeded the Tolerable Upper Level. Conclusion: Vitamin and mineral intake in Spain should be improved, especially in late childhood and adolescence. Nutritional intervention and educational strategies are needed to promote healthy eating habits and correct micronutrient inadequacies in Spanish children and adolescents.
Association between Serum Vitamin D Levels and Sleep Disturbance in Hemodialysis Patients
Authors: Han Bin , Zhu Fu-Xiang , Shi Chao , Wu Heng-Lan , Gu Xiao-Hong
Published: February 2017

Sleep disturbance is a frequent and serious complication of hemodialysis (HD). Low serum vitamin D levels have been associated with sleep quality in non-HD subjects. Our aim was to examine the possible association between serum vitamin D levels and the presence of sleep disturbance in HD patients. We recruited 141 HD patients at the HD center of the First Affiliated Hospital of Jiaxing University during 2014–2015. Serum levels of 25-hydroxyvitamin D (25(OH)D) were determined by the competitive protein-binding assay. Sleep quality was measured using the Pittsburgh Sleep Quality Index (PSQI). Demographic, clinical and laboratory data were recorded. Meanwhile, 117 healthy control subjects were also recruited and underwent measurement of 25(OH)D. Eighty-eight patients (62.4%) had sleep disturbance (PSQI scores ≥ 5). Patients with sleep disturbance showed lower levels of 25(OH)D as compared to those without sleep disturbance (85.6 ± 37.4 vs. 39.1 ± 29.1 nmol/L, p < 0.001). In multivariate analyses, serum levels of 25(OH)D (≤48.0 nmol/L) were independently associated with sleep disturbance in HD patients (OR 9.897, 95% CI 3.356–29.187, p < 0.001) after adjustment for possible variables. Our study demonstrates that low serum levels of vitamin D are independently associated with sleep disturbance in HD patients, but the finding needs to be confirmed in future experimental and clinical studies.
Transcriptomic Analysis Reveals Selective Metabolic Adaptation of Streptococcus suis to Porcine Blood and Cerebrospinal Fluid
Authors: Koczula Anna , Jarek Michael , Visscher Christian , Valentin-Weigand Peter , Goethe Ralph , Willenborg Jörg
Published: February 2017

Streptococcus suis is a zoonotic pathogen that can cause severe pathologies such as septicemia and meningitis in its natural porcine host as well as in humans. Establishment of disease requires not only virulence of the infecting strain but also an appropriate metabolic activity of the pathogen in its host environment. However, it is yet largely unknown how the streptococcal metabolism adapts to the different host niches encountered during infection. Our previous isotopologue profiling studies on S. suis grown in porcine blood and cerebrospinal fluid (CSF) revealed conserved activities of central carbon metabolism in both body fluids. On the other hand, they suggested differences in the de novo amino acid biosynthesis. This prompted us to further dissect S. suis adaptation to porcine blood and CSF by RNA deep sequencing (RNA-seq). In blood, the majority of differentially expressed genes were associated with transport of alternative carbohydrate sources and the carbohydrate metabolism (pentose phosphate pathway, glycogen metabolism). In CSF, predominantly genes involved in the biosynthesis of branched-chain and aromatic amino acids were differentially expressed. Especially, isoleucine biosynthesis seems to be of major importance for S. suis in CSF because several related biosynthetic genes were more highly expressed. In conclusion, our data revealed niche-specific metabolic gene activity which emphasizes a selective adaptation of S. suis to host environments.
An Improved Syringe Agroinfiltration Protocol to Enhance Transformation Efficiency by Combinative Use of 5-Azacytidine, Ascorbate Acid and Tween-20
Authors: Zhao Huimin , Tan Zilong , Wen Xuejing , Wang Yucheng
Published: February 2017

Syringe infiltration is an important transient transformation method that is widely used in many molecular studies. Owing to the wide use of syringe agroinfiltration, it is important and necessary to improve its transformation efficiency. Here, we studied the factors influencing the transformation efficiency of syringe agroinfiltration. The pCAMBIA1301 was transformed into Nicotiana benthamiana leaves for investigation. The effects of 5-azacytidine (AzaC), Ascorbate acid (ASC) and Tween-20 on transformation were studied. The β-glucuronidase (GUS) expression and GUS activity were respectively measured to determine the transformation efficiency. AzaC, ASC and Tween-20 all significantly affected the transformation efficiency of agroinfiltration, and the optimal concentrations of AzaC, ASC and Tween-20 for the transgene expression were identified. Our results showed that 20 μM AzaC, 0.56 mM ASC and 0.03% (v/v) Tween-20 is the optimal concentration that could significantly improve the transformation efficiency of agroinfiltration. Furthermore, a combined supplement of 20 μM AzaC, 0.56 mM ASC and 0.03% Tween-20 improves the expression of transgene better than any one factor alone, increasing the transgene expression by more than 6-fold. Thus, an optimized syringe agroinfiltration was developed here, which might be a powerful method in transient transformation analysis.
Mechanical and Electrical Properties of Sulfur-Containing Polymeric Materials Prepared via Inverse Vulcanization
Authors: Diez Sergej , Hoefling Alexander , Theato Patrick , Pauer Werner
Published: February 2017

Recently, new methods have been developed for the utilization of elemental sulfur as a feedstock for novel polymeric materials. One promising method is the inverse vulcanization, which is used to prepare polymeric structures derived from sulfur and divinyl comonomers. However, the mechanical and electrical properties of the products are virtually unexplored. Hence, in the present study, we synthesized a 200 g scale of amorphous, hydrophobic as well as translucent, hyperbranched polymeric sulfur networks that provide a high thermal resistance (>220 °C). The polymeric material properties of these sulfur copolymers can be controlled significantly by varying the monomers as well as the feed content. The investigated comonomers are divinylbenzene (DVB) and 1,3-diisopropenylbenzene (DIB). Plastomers with low elastic content and high shape retention containing 12.5%–30% DVB as well as low viscose waxy plastomers with a high flow behavior containing a high DVB content of 30%–35% were obtained. Copolymers with 15%–30% DIB act, on the one hand, as thermoplastics and, on the other hand, as vitreous thermosets with a DIB of 30%–35%. Results of the thermogravimetric analysis (TGA), the dynamic scanning calorimetry (DSC) and mechanical characterization, such as stress–strain experiments and dynamic mechanical thermal analysis, are discussed with the outcome that they support the assumption of a polymeric cross-linked network structure in the form of hyper-branched polymers.
Spatial-Temporal Characteristics and Climatic Responses of Water Level Fluctuations of Global Major Lakes from 2002 to 2010
Authors: Tan Chao , Ma Mingguo , Kuang Honghai
Published: February 2017

As one of the most important geographical units affected by global climate change, lakes are sensitive to climatic changes and are considered “indicators” of climate and the environment. In this study, changes in the spatial-temporal characteristics of the water levels of 204 global major lakes are systematically analyzed using satellite altimetry data (Hydroweb product) from 2002 to 2010. Additionally, the responses of the major global lake levels to climatic fluctuations are analyzed using Global Land Surface Assimilation System (GLDAS) data (temperature and precipitation). The results show that the change rates of most global lakes exceed 0, which means that the lake levels of these lakes are rising. The change rates of the lake levels are between −0.3~0.3 m/a, which indicates that the rate of change in the water-level of most lakes is not obvious. A few lakes have a particularly sharp change rate, between −5.84~−2 m/a or 0.7~1.87 m/a. Lakes with increasing levels are mainly located in the mountain and plateau regions, and the change rates in the coastal highlands are more evident. The global temperatures rise by a change rate of 0.0058 °C/a, while the global precipitation decreases by a change rate of −0.6697 mm/a. However, there are significant regional differences in both temperature and precipitation. In addition, the impact of precipitation on the water level of lakes is significant and straightforward, while the impact of temperature is more complex. A study of lake levels on a global scale would be quite useful for a better understanding of the impact which climate change has on surface water resources.
Combining Airborne Laser Scanning and Aerial Imagery Enhances Echo Classification for Invasive Conifer Detection
Authors: Dash Jonathan , Pearse Grant , Watt Michael , Paul Thomas
Published: February 2017

The spread of exotic conifers from commercial plantation forests has significant economic and ecological implications. Accurate methods for invasive conifer detection are required to enable monitoring and guide control. In this research, we combined spectral information from aerial imagery with data from airborne laser scanning (ALS) to develop methods to identify invasive conifers using remotely-sensed data. We examined the effect of ALS pulse density and the height threshold of the training dataset on classification accuracy. The results showed that adding spectral values to the ALS metrics/variables in the training dataset led to significant increases in classification accuracy. The most accurate models (kappa range of 0.773–0.837) had either four or five explanatory variables, including ALS elevation, the near-infrared band and different combinations of ALS intensity and red and green bands. The best models were found to be relatively invariant to changes in pulse density (1–21 pls/m2) or the height threshold (0–2 m) used for the inclusion of data in the training dataset. This research has extended and improved the methods for scattered single tree detection and offered valuable insight into campaign settings for the monitoring of invasive conifers (tree weeds) using remote sensing approaches.
A Review of Wearable Technologies for Elderly Care that Can Accurately Track Indoor Position, Recognize Physical Activities and Monitor Vital Signs in Real Time
Authors: Wang Zhihua , Yang Zhaochu , Dong Tao
Published: February 2017

Rapid growth of the aged population has caused an immense increase in the demand for healthcare services. Generally, the elderly are more prone to health problems compared to other age groups. With effective monitoring and alarm systems, the adverse effects of unpredictable events such as sudden illnesses, falls, and so on can be ameliorated to some extent. Recently, advances in wearable and sensor technologies have improved the prospects of these service systems for assisting elderly people. In this article, we review state-of-the-art wearable technologies that can be used for elderly care. These technologies are categorized into three types: indoor positioning, activity recognition and real time vital sign monitoring. Positioning is the process of accurate localization and is particularly important for elderly people so that they can be found in a timely manner. Activity recognition not only helps ensure that sudden events (e.g., falls) will raise alarms but also functions as a feasible way to guide people’s activities so that they avoid dangerous behaviors. Since most elderly people suffer from age-related problems, some vital signs that can be monitored comfortably and continuously via existing techniques are also summarized. Finally, we discussed a series of considerations and future trends with regard to the construction of “smart clothing” system.
An Optoelectronic Equivalent Narrowband Filter for High Resolution Optical Spectrum Analysis
Authors: Feng Kunpeng , Cui Jiwen , Dang Hong , Wu Weidong , Sun Xun , Jiang Xuelin , Tan Jiubin
Published: February 2017

To achieve a narrow bandwidth optical filter with a wide swept range for new generation optical spectrum analysis (OSA) of high performance optical sensors, an optoelectronic equivalent narrowband filter (OENF) was investigated and a swept optical filter with bandwidth of several MHz and sweep range of several tens of nanometers was built using electric filters and a sweep laser as local oscillator (LO). The principle of OENF is introduced and analysis of the OENF system is presented. Two electric filters are optimized to be RBW filters for high and medium spectral resolution applications. Both simulations and experiments are conducted to verify the OENF principle and the results show that the power uncertainty is less than 1.2% and the spectral resolution can reach 6 MHz. Then, a real-time wavelength calibration system consisting of a HCN gas cell and Fabry–Pérot etalon is proposed to guarantee a wavelength accuracy of ±0.4 pm in the C-band and to reduce the influence of phase noise and nonlinear velocity of the LO sweep. Finally, OSA experiments on actual spectra of various optical sensors are conducted using the OENF system. These experimental results indicate that OENF system has an excellent capacity for the analysis of fine spectrum structures.
A Novel Strain-Based Method to Estimate Tire Conditions Using Fuzzy Logic for Intelligent Tires
Authors: Garcia-Pozuelo Daniel , Olatunbosun Oluremi , Yunta Jorge , Yang Xiaoguang , Diaz Vicente
Published: February 2017

The so-called intelligent tires are one of the most promising research fields for automotive engineers. These tires are equipped with sensors which provide information about vehicle dynamics. Up to now, the commercial intelligent tires only provide information about inflation pressure and their contribution to stability control systems is currently very limited. Nowadays one of the major problems for intelligent tire development is how to embed feasible and low cost sensors to obtain reliable information such as inflation pressure, vertical load or rolling speed. These parameters provide key information for vehicle dynamics characterization. In this paper, we propose a novel algorithm based on fuzzy logic to estimate the mentioned parameters by means of a single strain-based system. Experimental tests have been carried out in order to prove the suitability and durability of the proposed on-board strain sensor system, as well as its low cost advantages, and the accuracy of the obtained estimations by means of fuzzy logic.
New Approaches to Exciting Exergame-Experiences for People with Motor Function Impairments
Authors: Eckert Martina , Gómez-Martinho Ignacio , Meneses Juan , Martínez José-Fernán
Published: February 2017

The work presented here suggests new ways to tackle exergames for physical rehabilitation and to improve the players’ immersion and involvement. The primary (but not exclusive) purpose is to increase the motivation of children and adolescents with severe physical impairments, for doing their required exercises while playing. The proposed gaming environment is based on the Kinect sensor and the Blender Game Engine. A middleware has been implemented that efficiently transmits the data from the sensor to the game. Inside the game, different newly proposed mechanisms have been developed to distinguish pure exercise-gestures from other movements used to control the game (e.g., opening a menu). The main contribution is the amplification of weak movements, which allows the physically impaired to have similar gaming experiences as the average population. To test the feasibility of the proposed methods, four mini-games were implemented and tested by a group of 11 volunteers with different disabilities, most of them bound to a wheelchair. Their performance has also been compared to that of a healthy control group. Results are generally positive and motivating, although there is much to do to improve the functionalities. There is a major demand for applications that help to include disabled people in society and to improve their life conditions. This work will contribute towards providing them with more fun during exercise.
Optical Sensor of Thermal Gas Flow Based on Fiber Bragg Grating
Authors: Jiang Xu , Wang Keda , Li Junqing , Zhan Hui , Song Zhenan , Che Guohang , Lyu Guohui
Published: February 2017

This paper aims at solving the problem of explosion proof in measurement of thermal gas flow using electronic sensor by presenting a new type of flow sensor by optical fiber heating. A measuring unit based on fiber Bragg grating (FBG) for fluid temperature and a unit for heat dissipation are designed to replace the traditional electronic sensors. The light in C band from the amplified spontaneous emission (ASE) light source is split, with one part used to heat the absorbing coating and the other part used in the signal processing unit. In the heating unit, an absorbing coating is introduced to replace the traditional resistance heating module to minimize the risk of explosion. The measurement results demonstrate a fine consistency between the flow and temperature difference in simulation. The method to enhance the measurement resolution of flow is also discussed.
Collagen-Gold Nanoparticle Conjugates for Versatile Biosensing
Authors: Unser Sarah , Holcomb Samuel , Cary ReJeana , Sagle Laura
Published: February 2017

Integration of noble metal nanoparticles with proteins offers promising potential to create a wide variety of biosensors that possess both improved selectivity and versatility. The multitude of functionalities that proteins offer coupled with the unique optical properties of noble metal nanoparticles can allow for the realization of simple, colorimetric sensors for a significantly larger range of targets. Herein, we integrate the structural protein collagen with 10 nm gold nanoparticles to develop a protein-nanoparticle conjugate which possess the functionality of the protein with the desired colorimetric properties of the nanoparticles. Applying the many interactions that collagen undergoes in the extracellular matrix, we are able to selectively detect both glucose and heparin with the same collagen-nanoparticle conjugate. Glucose is directly detected through the cross-linking of the collagen fibrils, which brings the attached nanoparticles into closer proximity, leading to a red-shift in the LSPR frequency. Conversely, heparin is detected through a competition assay in which heparin-gold nanoparticles are added to solution and compete with heparin in the solution for the binding sites on the collagen fibrils. The collagen-nanoparticle conjugates are shown to detect both glucose and heparin in the physiological range. Lastly, glucose is selectively detected in 50% mouse serum with the collagen-nanoparticle devices possessing a linear range of 3–25 mM, which is also within the physiologically relevant range.
Differences in the Dominant and Non-Dominant Knee Valgus Angle in Junior Elite and Amateur Soccer Players after Unilateral Landing
Authors: Ludwig Oliver , Simon Steven , Piret Joe , Becker Stephan , Marschall Franz
Published: February 2017

More than 70% of all knee injuries in soccer occur in non-contact situations. It is known that increased lower limb dynamic knee valgus is associated with such situations. Little has been found out about differences in knee kinematics of the dominant (kicking) and non-dominant (supporting) leg during a single leg landing. A total of 114 male adolescent soccer players (age 14.6 ± 1.1 years) from elite (N = 66) and amateur soccer clubs (N = 48) performed a single leg drop landing down from a box. For each leg, the two-dimensional dynamic knee valgus angle (DKVA) was calculated. Paired t-tests were used to statistically determine significant differences between dominant and non-dominant leg DKVA, and t-tests were calculated between the two performance groups. Statistically significant differences (p < 0.05) were identified for the DKVA between the dominant and non-dominant leg for both amateur and elite players, showing a greater DKVA for the dominant leg. Group differences for the DKVA between amateur and elite players were not found, neither for the dominant, nor for the non-dominant leg. It can be concluded that the non-dominant leg showed more stable dynamics than the dominant leg during unilateral landing regardless of the player’s performance level. This could be due to adaptions to sport-specific requirements. Therefore, it is recommended that programs to prevent knee injuries among soccer players consider the dynamics of each leg individually.