bluuebunny/crossref_metadata_2025 huggingface dataset:
Proteomics Reveals How the Tardigrade Damage Suppressor Protein Teaches Transfected Human Cells to Survive UV-C Stress
Authors: Shaba Enxhi , Landi Claudia , Marzocchi Carlotta , Vantaggiato Lorenza , Bini Luca , Ricci Claudia , Cantara Silvia
Published: July 2023

The genome sequencing of the tardigrade Ramazzottius varieornatus revealed a unique nucleosome-binding protein named damage suppressor (Dsup), which was discovered to be crucial for the extraordinary abilities of tardigrades in surviving extreme stresses, such as UV. Evidence in Dsup-transfected human cells suggests that Dsup mediates an overall response in DNA damage signaling, DNA repair, and cell cycle regulation, resulting in an acquired resistance to stress. Given these promising outcomes, our study attempts to provide a wider comprehension of the molecular mechanisms modulated by Dsup in human cells and to explore the Dsup-activated molecular pathways under stress. We performed a differential proteomic analysis of Dsup-transfected and control human cells under basal conditions and at 24 h recovery after exposure to UV-C. We demonstrate via enrichment and network analyses, for the first time, that even in the absence of external stimuli, and more significantly, after stress, Dsup activates mechanisms involved with the unfolded protein response, the mRNA processing and stability, cytoplasmic stress granules, the DNA damage response, and the telomere maintenance. In conclusion, our results shed new light on Dsup-mediated protective mechanisms and increases our knowledge of the molecular machineries of extraordinary protection against UV-C stress.
Cell Death and Neurodegenerative Diseases: Mechanisms and Cytoprotective Molecules
Authors: Vejux Anne
Published: July 2023

A neurodegenerative disease is a pathological condition affecting neurons, condemning them to death [...]
Immune System and Hepatocellular Carcinoma (HCC): New Insights into HCC Progression
Authors: Kotsari Maria , Dimopoulou Vassiliki , Koskinas John , Armakolas Athanasios
Published: July 2023

According to the WHO’s recently released worldwide cancer data for 2020, liver cancer ranks sixth in morbidity and third in mortality among all malignancies. Hepatocellular carcinoma (HCC), the most common kind of liver cancer, accounts approximately for 80% of all primary liver malignancies and is one of the leading causes of death globally. The intractable tumor microenvironment plays an important role in the development and progression of HCC and is one of three major unresolved issues in clinical practice (cancer recurrence, fatal metastasis, and the refractory tumor microenvironment). Despite significant advances, improved molecular and cellular characterization of the tumor microenvironment is still required since it plays an important role in the genesis and progression of HCC. The purpose of this review is to present an overview of the HCC immune microenvironment, distinct cellular constituents, current therapies, and potential immunotherapy methods.
Diversity Analysis and Function Prediction of Bacterial Communities in the Different Colored Pericarp of Citrus reticulata cv. ‘Shatangju’ Due to ‘Candidatus Liberibacter asiaticus’ Infection
Authors: Wang Feiyan , Zhu Congyi , Zhang Ruimin , Huang Yongjing , Wu Wen , Chen Jiezhong , Zeng Jiwu
Published: July 2023

Huanglongbing (HLB), caused by the Candidatus Liberibacter spp., is the most devastating disease in the citrus industry. HLB significantly affects and alters the microbial community structure or potential function of the microbial community of leaves and roots. However, it is unknown how the microbial community structure of the pericarp with different pigments is affected by Candidatus Liberibacter asiaticus (CLas). This study identified the enriched taxa of the microbial community in the citrus pericarp with normal or abnormal pigment and determine the effects of HLB on the pericarp microbial community using 16S rRNA-seq. The alpha and beta diversity and composition of microbial communities were significantly different between normal and abnormal pigment pericarp tissues of ripe fruits infected by CLas. Firmicutes, Actinobacteriota, Bacteroidota, Acidobacteriota, and Desulfobacterota dominated the pericarp microbiota composition in WDYFs (whole dark yellow fruits) samples. The relative abundance of most genera in WDYFs was higher than 1%, such as Burkholderia, and Pelomonas. However, with the exception of the HLB pathogen, the relative abundance of most genera in the abnormal-colored pericarp samples was less than 1%. CLas decreased the relative abundance of pericarp taxonomic. The predicted function of microbial was more plentiful and functional properties in the WDYF sample, such as translation, ribosomal structure and biogenesis, amino acid transport and metabolism, energy production and conversion, and some other clusters of orthologous groups (COG) except for cell motility. The results of this study offer novel insights into understanding the composition of microbial communities of the CLas-affected citrus pericarps and contribute to the development of biological control strategies for citrus against Huanglongbing.
Triplin: Mechanistic Basis for Voltage Gating
Authors: Colombini Marco , Liu Patrick , Dee Chase
Published: July 2023

The outer membrane of Gram-negative bacteria contains a variety of pore-forming structures collectively referred to as porins. Some of these are voltage dependent, but weakly so, closing at high voltages. Triplin, a novel bacterial pore-former, is a three-pore structure, highly voltage dependent, with a complex gating process. The three pores close sequentially: pore 1 at positive potentials, 2 at negative and 3 at positive. A positive domain containing 14 positive charges (the voltage sensor) translocates through the membrane during the closing process, and the translocation is proposed to take place by the domain entering the pore and thus blocking it, resulting in the closed conformation. This mechanism of pore closure is supported by kinetic measurements that show that in the closing process the voltage sensor travels through most of the transmembrane voltage before reaching the energy barrier. Voltage-dependent blockage of the pores by polyarginine, but not by a 500-fold higher concentrations of polylysine, is consistent with the model of pore closure, with the sensor consisting mainly of arginine residues, and with the presence, in each pore, of a complementary surface that serves as a binding site for the sensor.
Biochemical Defense Responses in Red Rice Genotypes Possessing Differential Resistance to Brown Planthopper, Nilaparvata lugens (Stål)
Authors: Pati Prajna , Jena Mayabini , Bhattacharya Swarnali , Behera Santhosh Kumar , Pal Subhajit , Shivappa Raghu , Dhar Tapamay
Published: July 2023

The brown planthopper [Nilaparvata lugens (Stål.)] is one of the most destructive insect pests in all the rice-growing regions of the world. The pest is complicated to manage through the blanket application of chemical pesticides. The development of stable, durable N. lugens-resistant rice varieties is the most economical and efficient strategy to manage the pest. Landraces of red rice genotypes possess numerous nutritional and stress-resistant properties, though an exclusive study on the same is yet to be carried out. In the present study, we evaluated 28 red rice genotypes, along with two resistance checks and one susceptibility check, for their resistance to N. lugens. These promising lines revealed differential responses in the defense mechanism against the pest. The resistant accessions showed a greater accumulation of phenols, peroxidase, polyphenol oxidase, catalase, and superoxide dismutase under N. lugens-stressed conditions. However, the concentration of soluble proteins was substantially decreased in all the test genotypes. The concentration of crude silica was at maximum in highly resistant genotypes. Six red rice genotypes, namely Mata Meher, Manipuri Black, Hermonona, Sonahanan, Bavdi, and Bacharya Khuta fall under the highly resistant category, and can be utilized as valuable sources of resistance in breeding programs.
Antibacterial and Antihemolytic Activity of New Biomaterial Based on Glycyrrhizic Acid and Quercetin (GAQ) against Staphylococcus aureus
Authors: Olchowik-Grabarek Ewa , Czerkas Krzysztof , Matchanov Alimjon Davletboevich , Esanov Rahmat Sulton , Matchanov Umarbek Davlatboevich , Zamaraeva Maria , Sekowski Szymon
Published: July 2023

The goal of this study is to obtain and characterize the complex of quercetin with glycyrrhizic acid, which is known to serve as a drug delivery system. Quercetin is a flavonoid with a wide range of biological activities, including an antimicrobial effect. However, quercetin instability and low bioavailability that limits its use in medical practice makes it necessary to look for new nanoformulations of it. The formation of the GAQ complex (2:1) was confirmed by using UV and FT-IR spectroscopies. It was found that the GAQ exhibited antimicrobial and antihemolytical activities against S. aureus bacteria and its main virulent factor—α-hemolysin. The IC50 value for the antihemolytical effect of GAQ was 1.923 ± 0.255 µg/mL. Using a fluorescence method, we also showed that the GAQ bound tightly to the toxin that appears to underlie its antihemolytic activity. In addition, another mechanism of the antihemolytic activity of the GAQ against α-hemolysin was shown, namely, its ability to increase the rigidity of the outer layer of the erythrocyte membrane and thus inhibit the incorporation of α-hemolysin into the target cells, increasing their resistance to the toxin. Both of these effects of GAQ were observed at concentrations below the MIC value for S. aureus growth, indicating the potential of the complex as an antivirulence agent.
Antibacterial Biomaterial Based on Bioglass Modified with Copper for Implants Coating
Authors: Hammami Imen , Gavinho Sílvia Rodrigues , Jakka Suresh Kumar , Valente Manuel Almeida , Graça Manuel Pedro Fernandes , Pádua Ana Sofia , Silva Jorge Carvalho , Sá-Nogueira Isabel , Borges João Paulo
Published: July 2023

Biofilm-related implant infections pose a substantial threat to patients, leading to inflammation in the surrounding tissue, and often resulting in implant loss and the necessity for additional surgeries. Overcoming this implantology challenge is crucial to ensure the success and durability of implants. This study shows the development of antibacterial materials for implant coatings by incorporating copper into 45S5 Bioglass®. By combining the regenerative properties of Bioglass® with the antimicrobial effects of copper, this material has the potential to prevent infections, enhance osseointegration and improve the long-term success of implants. Bioglasses modified with various concentrations of CuO (from 0 to 8 mol%) were prepared with the melt-quenching technique. Structural analysis using Raman and FTIR spectroscopies did not reveal significant alterations in the bioglasses structure with the addition of Cu. The antibacterial activity of the samples was assessed against Gram-positive and Gram-negative bacteria, and the results demonstrated significant inhibition of bacterial growth for the bioglass with 0.5 mol% of CuO. Cell viability studies indicated that the samples modified with up to 4 mol% of CuO maintained good cytocompatibility with the Saos-2 cell line at extract concentrations up to 25 mg/mL. Furthermore, the bioactivity assessment demonstrated the formation of a calcium phosphate (CaP)-rich layer on the surfaces of all bioglasses within 24 h. Our findings show that the inclusion of copper in the bioglass offers a significant enhancement in its potential as a coating material for implants, resulting in notable advancements in both antibacterial efficacy and osteointegration properties.
Bioprinted 3D Bionic Scaffolds with Pancreatic Islets as a New Therapy for Type 1 Diabetes—Analysis of the Results of Preclinical Studies on a Mouse Model
Authors: Klak Marta , Wszoła Michał , Berman Andrzej , Filip Anna , Kosowska Anna , Olkowska-Truchanowicz Joanna , Rachalewski Michał , Tymicki Grzegorz , Bryniarski Tomasz , Kołodziejska Marta , Dobrzański Tomasz , Ujazdowska Dominika , Wejman Jarosław , Uhrynowska-Tyszkiewicz Izabela , Kamiński Artur
Published: July 2023

Recently, tissue engineering, including 3D bioprinting of the pancreas, has acquired clinical significance and has become an outstanding potential method of customized treatment for type 1 diabetes mellitus. The study aimed to evaluate the function of 3D-bioprinted pancreatic petals with pancreatic islets in the murine model. A total of 60 NOD-SCID (Nonobese diabetic/severe combined immunodeficiency) mice were used in the study and divided into three groups: control group; IsletTx (porcine islets transplanted under the renal capsule); and 3D bioprint (3D-bioprinted pancreatic petals with islets transplanted under the skin, on dorsal muscles). Glucose, C-peptide concentrations, and histological analyses were performed. In the obtained results, significantly lower mean fasting glucose levels (mg/dL) were observed both in a 3D-bioprint group and in a group with islets transplanted under the renal capsule when compared with untreated animals. Differences were observed in all control points: 7th, 14th, and 28th days post-transplantation (129, 119, 118 vs. 140, 139, 140; p < 0.001). Glucose levels were lower on the 14th and 28th days in a group with bioprinted petals compared to the group with islets transplanted under the renal capsule. Immunohistochemical staining indicated the presence of secreted insulin-living pancreatic islets and neovascularization within 3D-bioprinted pancreatic petals after transplantation. In conclusion, bioprinted bionic petals significantly lowered plasma glucose concentration in studied model species.
Varroa Destructor Classification Using Legendre–Fourier Moments with Different Color Spaces
Authors: Noriega-Escamilla Alicia , Camacho-Bello César J. , Ortega-Mendoza Rosa M. , Arroyo-Núñez José H. , Gutiérrez-Lazcano Lucia
Published: July 2023

Bees play a critical role in pollination and food production, so their preservation is essential, particularly highlighting the importance of detecting diseases in bees early. The Varroa destructor mite is the primary factor contributing to increased viral infections that can lead to hive mortality. This study presents an innovative method for identifying Varroa destructors in honey bees using multichannel Legendre–Fourier moments. The descriptors derived from this approach possess distinctive characteristics, such as rotation and scale invariance, and noise resistance, allowing the representation of digital images with minimal descriptors. This characteristic is advantageous when analyzing images of living organisms that are not in a static posture. The proposal evaluates the algorithm’s efficiency using different color models, and to enhance its capacity, a subdivision of the VarroaDataset is used. This enhancement allows the algorithm to process additional information about the color and shape of the bee’s legs, wings, eyes, and mouth. To demonstrate the advantages of our approach, we compare it with other deep learning methods, in semantic segmentation techniques, such as DeepLabV3, and object detection techniques, such as YOLOv5. The results suggest that our proposal offers a promising means for the early detection of the Varroa destructor mite, which could be an essential pillar in the preservation of bees and, therefore, in food production.
Periodontal Pathogens and Adverse Pregnancy Outcomes: A Narrative Review
Authors: AlSharief Mishali , Alabdurubalnabi Esraa
Published: July 2023

Periodontal disease is a multi-microbial infection of the teeth-supporting apparatus that manifests as clinical attachment loss and alveolar bone loss. The association between periodontal disease and systemic diseases has been proposed in the literature owing to the former’s chronic state of inflammation, and adverse pregnancy outcomes are no exception. As a result of periodontal pathogen invasion, a series of systemic inflammatory and immunologic events affecting the safety of the fetoplacental unit may unfold. This may be further exaggerated by physiologic hormonal and metabolic fluctuations during pregnancy. This can not only negatively affect the gestation period and consequently cause preterm low weight but also complicate the pregnancy via preeclampsia and gestational diabetes. This narrative review article aims to provide a summary of relevant available evidence pertinent to the relationship between periodontal diseases, associated periodontal pathogens and virulence mechanisms mediated by pro-inflammatory cytokines and prostaglandins, and adverse pregnancy outcomes. Furthermore, this article highlights some of the literature addressing the impact of periodontal therapy interventions and pregnancy outcomes.
Testing the Performance of the Azo-Polyimide Supramolecular Systems as Substrate for Sensors Based on Platinum Electrodes
Authors: Sava Ion , Asandulesa Mihai , Barzic Andreea Irina , Albu Raluca Marinica , Stoica Iuliana
Published: July 2023

Azo-polyimide films with supramolecular structure were obtained by casting onto glass plates a mixture based on polyamidic acid and different quantities of azochromophore, followed by thermal treatment to realize the final azo-polyimide structure. The dielectric characteristics of the supramolecular structure of polymer films were investigated by broad-band dielectric spectroscopy measurements at different temperatures and frequencies. The free-standing films proved to be flexible and tough and maintained their integrity after repeated bending. The work of adhesion at the polymer/platinum interface was calculated after the evaluation of the surface energy parameters before and after plasma treatment. Atomic force microscopy was used to image the surface morphology, the evolution of the roughness parameters, and the adhesion force between the platinum-covered tip and the polymer surface, registered at the nanoscale with the quantity of the azo dye introduced in the system. The simulation of the columnar growth of a platinum layer was made to provide information about the deposition parameters that should be used for optimal results in the deposition of platinum electrodes for sensors.
Effect of a Fine Fraction on Dynamic Properties of Recycled Concrete Aggregate as a Special Anthropogenic Soil
Authors: Gabryś Katarzyna , Šadzevičius Raimondas , Dapkienė Midona , Ramukevičius Dainius , Sas Wojciech
Published: July 2023

The literature confirms that fine recycled concrete aggregate (fRCA) can be used as a replacement for natural soil in new concrete, offering many advantages. Despite these advantages, there are also critical barriers to the development of fRCA in new mixes. Among these, the first challenge is the variability of fRCA properties, in both physical, chemical, and mechanical terms. Many individual studies have been carried out on different RCA or fRCA properties, but little investigative work has been performed to analyze their dynamic properties. Therefore, the influence of the non-cohesive fine fraction content of RCA on the dynamic properties of this waste material, when used as a specific anthropogenic soil, has been studied in laboratory conditions, employing a standard resonant column apparatus, as well as piezoelectric elements. In the present research, special emphasis has been placed on the dynamic shear modulus, dynamic damping ratio, small-strain shear modulus, and small-strain damping ratio, as well as shear modulus degradation G(γ)/Gmax, the damping ratio increase D(γ)/Dmin, and the threshold shear strain amplitudes γtl and γtv. Artificially prepared fRCAs with varying fine fraction contents (0% ≤ FF ≤ 30%, within increments of 5%) have been tested at different pressures (p′ = 90, 180, and 270 kPa) and relative densities of Dr > 65%. This study also examined the effect of two tamping-based sample preparation methods, i.e., dry and wet tamping. The results presented herein indicate that the analyzed anthropogenic material, although derived from concrete and produced by human activities, behaves very similarly to natural aggregate when subjected to dynamic loading. The introduction of a fine fraction content to fRCA leads to changes in the dynamic properties of the tested mixture. Concrete material with lower stiffness but, at the same time, with stronger damping properties can be obtained. A fine fraction content of at least 30% is sufficient to cause a significant loss of stiffness and, at the same time, a significant increase in the damping properties of the mixture. This study can serve as a reference for designing fRCA mixtures in engineering applications.
Study on Porosity Defect Detection in Narrow Gap Laser Welding Based on Spectral Diagnosis
Authors: Liu Jinping , Xu Baoping , Feng Yingchao , Chen Peng , Yan Cancan , Li Zhuyuan , Yang Kaisong , She Kun , Huang Yiming
Published: July 2023

As an advanced connection technology for large thick-walled components, narrow gap laser welding has the advantages of small heat input and high efficiency and quality. However, porosity defects are prone to occur inside the weld due to the complex welding environment. In this study, the influence of the process parameters and pollutants such as water and oil on the porosity defect were explored. The action mechanism of water on the electron temperature and spectral intensity of the laser-induced plasma was analyzed. The results showed that the spectral intensity during narrow gap laser welding was weaker than that of flat plate butt welding. Under the optimal welding process conditions, the electron temperature during narrow gap laser self-fusion welding was calculated as 7413.3 K by the Boltzmann plot method. The electron density was 5.6714 × 1015 cm−3, conforming to the thermodynamic equilibrium state. With six groups of self-fusion welding parameters, only sporadic porosity defects were observed according to the X-ray detection. When there was water on the base metal surface, a large number of dense pores were observed on the weld surface and in the weld through X-ray inspection. Compared with the spectral data obtained under the normal process, the relative light intensity of the spectrometer in the whole band was reduced. The electron temperature decreased to the range of 6900 to 7200 K, while the electron density increased. The spectrum variation during narrow gap laser wire filling welding was basically the same as that of laser self-fusion welding. The porosity defects caused by water and oil pollutants in the laser welding could be effectively identified based on the intensity of the Fe I spectral lines.
Cobalt-Doped Bioactive Glasses for Biomedical Applications: A Review
Authors: Baino Francesco , Montazerian Maziar , Verné Enrica
Published: July 2023

Improving angiogenesis is the key to the success of most regenerative medicine approaches. However, how and to which extent this may be performed is still a challenge. In this regard, cobalt (Co)-doped bioactive glasses show promise being able to combine the traditional bioactivity of these materials (especially bone-bonding and osteo-stimulatory properties) with the pro-angiogenic effect associated with the release of cobalt. Although the use and local delivery of Co2+ ions into the body have raised some concerns about the possible toxic effects on living cells and tissues, important biological improvements have been highlighted both in vitro and in vivo. This review aims at providing a comprehensive overview of Co-releasing glasses, which find biomedical applications as various products, including micro- and nanoparticles, composites in combination with biocompatible polymers, fibers and porous scaffolds. Therapeutic applications in the field of bone repair, wound healing and cancer treatment are discussed in the light of existing experimental evidence along with the open issues ahead.
Strain Rate Sensitivity and Constitutive Law of Closed-Cell PVC Foams under Shock
Authors: Xue Bin , Zhou Yong
Published: July 2023

The mechanisms of the strain rate dependence of closed-cell PVC foams under shock were numerically studied based on a cell-based model combined with the Coupled Eulerian–Lagrangian (CEL) method in this paper. The strain rate effect of the base material and the entrapped gas effect were focused. The results show that the strain rate effect of the base material has a significant influence on the stress magnitude in the regions before and after the shock front, and the entrapped gas mainly affects the velocity field. Both the strain rate effect of the base material and the entrapped gas have a notable influence on the strain distribution. Taking PVC foam with a relative density of 0.07 as an example, the strain rate effect of the base material will increase the impact stress by 45% and reduce the impact strain by 0.04. The entrapped gas will reduce the impact strain by 0.18, and its effect on the impact stress can be ignored. Finally, two constitutive laws considering the strain rate effect and entrapped gas effect were proposed and compared for the PVC foam under shock with one based on the Hugoniot relationship and the other based on the D-RPH model.
The Effect of Clearance Angle on Tool Life, Cutting Forces, Surface Roughness, and Delamination during Carbon-Fiber-Reinforced Plastic Milling
Authors: Knápek Tomáš , Dvořáčková Štěpánka , Váňa Martin
Published: July 2023

This study aimed to investigate the effect of the clearance angle of the milling tool on wear, cutting forces, machined edge roughness, and delamination during non-contiguous milling of carbon-fiber-reinforced plastic (CFRP) composite panels with a twill weave and 90° fiber orientation. To achieve the objective of the study, it was first necessary to design suitable tools (6 mm diameter sintered carbide shank milling cutters) with a variety of clearance angles (8.4°, 12.4°, and 16.4°) and all the machinery and measuring equipment for the research to be carried out. Furthermore, measurement and evaluation methods for cutting tool wear, cutting forces, machined edge roughness, and delamination were developed. Last but not least, the results obtained during the research were summarized and evaluated. From the experiments conducted in this study, it was found that the tool clearance angle has a significant effect on tool wear, roughness of the machined surface, and delamination of the carbon fiber composite board. The tool with a clearance angle of 8.4° wore faster than the tool with a clearance angle of 16.4°. The same trend was observed for cutting force, machined surface roughness, and delamination. In this context, it was also shown that the cutting force increased as the tool wear increased, which in turn increased surface roughness and delamination. These results are of practical significance, not only in terms of the quality of the machined surface but also in terms of time, cost, and energy savings when machining CFRP composite materials.
Comparison of Wire and Non-Wire Localisation Techniques in Breast Cancer Surgery: A Review of the Literature with Pooled Analysis
Authors: Shirazi Shahram , Hajiesmaeili Hamed , Khosla Muskaan , Taj Saima , Sircar Tapan , Vidya Raghavan
Published: July 2023

Background and Objectives: Wide local excision is a common procedure in the treatment of breast cancer. Wire-guided localisation (WGL) has been the gold standard for many years; however, several issues have been identified with this technique, and therefore, wire-free techniques have been developed. This scoping review synthesises the available literature comparing wire-guided localisation with the wire-free techniques used in breast-conserving cancer surgery. Materials and Methods: Multiple databases including Pubmed and MEDLINE were used to search articles between 1 January 2000 and 31 December 2022. Terms included “breast neoplasms”, “margins of excision”, and “reoperation”. In total, 34/256 papers were selected for review. Comparisons were made between positive margins and re-excision rates of WGL with wire-free techniques including SAVI SCOUT, Magseed, ROLL, and RSL. Pooled p-values were calculated using chi-square testing to determine statistical significance. Results: Pooled analysis demonstrated statistically significant reductions in positive margins and re-excision rates when SAVI SCOUT, RSL, and ROLL were compared with WGL. When SAVI SCOUT was compared to WGL, there were fewer re-excisions {(8.6% vs. 18.8%; p = 0.0001) and positive margins (10.6% vs. 15.0%; p = 0.0105)}, respectively. This was also the case in the ROLL and RSL groups. When compared to WGL; lower re-excision rates and positive margins were noted {(12.6% vs. 20.8%; p = 0.0007), (17.0% vs. 22.9%; p = 0.0268)} for ROLL and for RSL, respectively {(6.8% vs. 14.9%),(12.36% vs. 21.4%) (p = 0.0001)}. Magseed localisation demonstrated lower rates of re-excision than WGL (13.44% vs. 15.42%; p = 0.0534), but the results were not statistically significant. Conclusions: SAVI SCOUT, Magseed, ROLL, and RSL techniques were reviewed. Pooled analysis indicates wire-free techniques, specifically SAVI SCOUT, ROLL, and RSL, provide statistically significant reductions in re-excision rates and positive margin rates compared to WGL. However, additional studies and systematic analysis are required to ascertain superiority between techniques.
Recent Advances in Electrochemical Biosensors for Food Control
Authors: Rizzotto Francesco , Khalife Majd , Hou Yanxia , Chaix Carole , Lagarde Florence , Scaramozzino Natale , Vidic Jasmina
Published: July 2023

The rapid and sensitive detection of food contaminants is becoming increasingly important for timely prevention and treatment of foodborne disease. In this review, we discuss recent developments of electrochemical biosensors as facile, rapid, sensitive, and user-friendly analytical devices and their applications in food safety analysis, owing to the analytical characteristics of electrochemical detection and to advances in the design and production of bioreceptors (antibodies, DNA, aptamers, peptides, molecular imprinted polymers, enzymes, bacteriophages, etc.). They can offer a low limit of detection required for food contaminants such as allergens, pesticides, antibiotic traces, toxins, bacteria, etc. We provide an overview of a broad range of electrochemical biosensing designs and consider future opportunities for this technology in food control.
Numerical and Experimental Investigation on a “Tai Chi”-Shaped Planar Passive Micromixer
Authors: Xia Annan , Shen Cheng , Wei Chengfeng , Meng Lingchen , Hu Zhiwen , Zhang Luming , Chen Mengyue , Li Liang , He Ning , Hao Xiuqing
Published: July 2023

(1) Background: Microfluidic chips have found extensive applications in multiple fields due to their excellent analytical performance. As an important platform for micro-mixing, the performance of micromixers has a significant impact on analysis accuracy and rate. However, existing micromixers with high mixing efficiency are accompanied by high pressure drop, which is not conducive to the integration of micro-reaction systems; (2) Methods: This paper proposed a novel “Tai Chi”-shaped planar passive micromixer with high efficiency and low pressure drop. The effect of different structural parameters was investigated, and an optimal structure was obtained. Simulations on the proposed micromixer and two other micromixers were carried out while mixing experiments on the proposed micromixer were performed. The experimental and simulation results were compared; (3) Results: The optimized values of the parameters were that the straight channel width w, ratio K of the outer and inner walls of the circular cavity, width ratio w1/w2 of the arc channel, and number N of mixing units were 200 μm, 2.9, 1/2, and 6, respectively. Moreover, the excellent performance of the proposed micromixer was verified when compared with the other two micromixers; (4) Conclusions: The mixing efficiency M at all Re studied was more than 50%, and at most Re, the M was nearly 100%. Moreover, the pressure drop was less than 18,000 Pa.
The Role of Probiotics in Skin Health and Related Gut–Skin Axis: A Review
Authors: Gao Ting , Wang Xiaoyu , Li Yixuan , Ren Fazheng
Published: July 2023

Aging skin, wrinkles, pigmentation, and dryness are problems that plague people, and researchers are working to solve them. Recent studies have shown that intestinal microbiota homeostasis can influence skin health, demonstrating the existence of a gut–skin axis. Recently, improving skin health through probiotic interventions has been proposed, and micro-ecological skin care is becoming a popular concept. By regulating skin health and gut–skin axis interactions, probiotics can be used as potential management tools to suppress and improve skin diseases in multiple ways, including decreasing oxidative stress, suppressing inflammatory responses, and keeping immune effects. The purpose of this paper is to provide a comprehensive review of the application and mechanisms of probiotic-mediated gut microbiota homeostasis in skin care and to offer a theoretical basis for the application of probiotics in skin care.
The Role of the Kynurenine Pathway in the Pathophysiology of Frailty, Sarcopenia, and Osteoporosis
Authors: Ballesteros Juan , Rivas Daniel , Duque Gustavo
Published: July 2023

Tryptophan is an essential nutrient required to generate vitamin B3 (niacin), which is mainly involved in energy metabolism and DNA production. Alterations in tryptophan metabolism could have significant effects on aging and musculoskeletal health. The kynurenine pathway, essential in tryptophan catabolism, is modulated by inflammatory factors that are increased in older persons, a process known as inflammaging. Osteoporosis, sarcopenia, osteosarcopenia, and frailty have also been linked with chronically increased levels of inflammatory factors. Due to the disruption of the kynurenine pathway by chronic inflammation and/or changes in the gut microbiota, serum levels of toxic metabolites are increased and are associated with the pathophysiology of those conditions. In contrast, anabolic products of this pathway, such as picolinic acid, have demonstrated a positive effect on skeletal muscle and bone. In addition, physical activity can modulate this pathway by promoting the secretion of anabolic kynurenines. According to the evidence collected, kynurenines could have a promising role as biomarkers for osteoporosis sarcopenia, osteosarcopenia, and frailty in older persons. In addition, some of these metabolites could become important targets for developing new pharmacological treatments for these conditions.
Effect of Black Garlic Consumption on Endothelial Function and Lipid Profile: A Before-and-After Study in Hypercholesterolemic and Non-Hypercholesterolemic Subjects
Authors: Villaño Débora , Marhuenda Javier , Arcusa Raúl , Moreno-Rojas José Manuel , Cerdá Begoña , Pereira-Caro Gema , Zafrilla Pilar
Published: July 2023

Background: Black garlic is obtained from raw garlic (Allium sativum L.), by a fermentation process, under humidity and heat treatment, showing a high concentration of organosulfur compounds, which have been related to benefits in the prevention or delay of cardiovascular diseases (CVDs). The objective of the research was to evaluate whether long-term consumption of black garlic improves endothelial function and lipid profile in subjects with hypercholesterolemia. Methods: Single center, controlled clinical trial with two branches: Hypercholesterolemia vs. Healthy condition. Sixty-two subjects of both sexes were distributed in two groups, the hypercholesterolemia group (n = 31) (total cholesterol (TC) range 200–300 mg/dL and low-density lipoprotein (LDL)-cholesterol range 135–175 mg/dL) and the healthy group (n = 31). The intervention consisted of the ingestion of 4 cloves of black garlic (12 g) daily for 12 weeks. Results: significant increases in Apolipoprotein (Apo)A1 occurred in both groups: Hypercholesterolemia (Δ 11.8 mg/dL p < 0.001) vs Healthy (Δ 11.1 mg/dL p < 0.001). Besides, significant reductions for endothelial adhesion molecules monocyte chemoattractant protein-1 (MCP-1) (Δ −121.5 pg/mL p = 0.007 vs. Δ −56.3 pg/mL p = 0.015), intracellular adhesion molecule-1 (ICAM-1) (Δ −39.3 ng/mL p < 0.001 vs. Δ 63.5 ng/mL p < 0.001), and vascular cyto-adhesion molecule-1 (VCAM-1) (Δ −144.4 ng/mL p < 0.001 vs. Δ −83.4 ng/mL p = 0.061) were observed, for hypercholesterolemic and healthy subjects, respectively. Conclusions: These data show that black garlic consumption could improve some parameters related to endothelial function and lipid profile, which may have a favorable impact on the risk of CVDs, although more long-term studies are necessary to confirm.
The Association between Dietary Fiber Intake and Serum Klotho Levels in Americans: A Cross-Sectional Study from the National Health and Nutrition Examination Survey
Authors: Liu Si , Wu Mingyang , Wang Yan , Xiang Lu , Luo Gang , Lin Qian , Xiao Lin
Published: July 2023

Background: Klotho is an aging-related marker closely associated with a number of diseases. A growing body of evidence suggests that dietary factors and lifestyle habits can impact serum Klotho levels. The effect of dietary fiber, a key component of a healthy diet, on the body’s serum Klotho levels has not been fully elucidated. Objective: The aim of this study was to explore the relationship between dietary fiber intake and serum Klotho levels in people aged 40–79 years in the United States. Methods: A total of 11,282 participants were included in this study, all from the National Health and Nutrition Examination Survey from 2007 to 2016. Dietary fiber intake was assessed by uniformly trained interviewers using the 24 h dietary recall method. Serum Klotho was quantified using commercially available ELISA kits manufactured by IBL International, Japan. The relationship between dietary fiber intake and serum Klotho levels was analyzed using a multiple linear regression model. Subsequently, the non-linear dose–response relationship between the two was further explored using a restricted cubic spline (RCS) model. Results: After adjusting for potential confounders, serum Klotho levels increased by 1.9% (95% confidence interval [CI]: 0.8%, 3.0%) for each interquartile range increase in dietary fiber intake in all participants. Considering dietary fiber intake as a categorical variable, serum Klotho levels were found to be 4.7% higher in participants in the highest quartile of dietary fiber intake than in those in the lowest quartile (95% CI: 1.8%, 7.6%). RCS plots depicted a non-linear positive correlation between dietary fiber intake and serum Klotho levels. Subgroup analysis revealed that the relationship between dietary fiber intake and serum Klotho levels was more pronounced in older (percentage change: 7.0%; 95% CI: 2.5%, 11.7%) and overweight and obese participants (percentage change: 4.9%; 95% CI: 1.5%, 8.4%). Conclusions: The results of this study showed that dietary fiber intake was significantly associated with serum Klotho levels in participants. This finding is yet to be further confirmed by prospective studies.
Topical Microemulsions: Skin Irritation Potential and Anti-Inflammatory Effects of Herbal Substances
Authors: Leanpolchareanchai Jiraporn , Teeranachaideekul Veerawat
Published: July 2023

Microemulsions (MEs) have gained prominence as effective drug delivery systems owing to their optical transparency, low viscosity, and thermodynamic stability. MEs, when stabilized with surfactants and/or co-surfactants, exhibit enhanced drug solubilization, prolonged shelf life, and simple preparation methods. This review examines the various types of MEs, explores different preparation techniques, and investigates characterization approaches. Plant extracts and bioactive compounds are well established for their utilization as active ingredients in the pharmaceutical and cosmetic industries. Being derived from natural sources, they serve as preferable alternatives to synthetic chemicals. Furthermore, they have demonstrated a wide range of therapeutic effects, including anti-inflammatory, antimicrobial, and antioxidant activities. However, the topical application of plant extracts and bioactive compounds has certain limitations, such as low skin absorption and stability. To overcome these challenges, the utilization of MEs enables enhanced skin absorption, thereby making them a valuable mode of administration. However, considering the significant surfactant content in MEs, this review evaluates the potential skin irritation caused by MEs containing herbal substances. Additionally, the review explores the topical application of MEs specifically for herbal substances, with an emphasis on their anti-inflammatory properties.
Ocular Delivery of Bimatoprost-Loaded Solid Lipid Nanoparticles for Effective Management of Glaucoma
Authors: Satyanarayana Sandeep Divate , Abu Lila Amr Selim , Moin Afrasim , Moglad Ehssan H. , Khafagy El-Sayed , Alotaibi Hadil Faris , Obaidullah Ahmad J. , Charyulu Rompicherla Narayana
Published: July 2023

Glaucoma is a progressive optic neuropathy characterized by a rise in the intraocular pressure (IOP) leading to optic nerve damage. Bimatoprost is a prostaglandin analogue used to reduce the elevated IOP in patients with glaucoma. The currently available dosage forms for Bimatoprost suffer from relatively low ocular bioavailability. The objective of this study was to fabricate and optimize solid lipid nanoparticles (SLNs) containing Bimatoprost for ocular administration for the management of glaucoma. Bimatoprost-loaded SLNs were fabricated by solvent evaporation/ultrasonication technique. Glyceryl Monostearate (GMS) was adopted as solid lipid and poloxamer 407 as surfactant. Optimization of SLNs was conducted by central composite design. The optimized formulation was assessed for average particle size, entrapment efficiency (%), zeta potential, surface morphology, drug release study, sterility test, isotonicity test, Hen’s egg test-chorioallantoic membrane (HET-CAM) test and histopathology studies. The optimized Bimatoprost-loaded SLNs formulation had an average size of 183.3 ± 13.3 nm, zeta potential of −9.96 ± 1.2 mV, and encapsulation efficiency percentage of 71.8 ± 1.1%. Transmission electron microscopy (TEM) study revealed the nearly smooth surface of formulated particles with a nano-scale size range. In addition, SLNs significantly sustained Bimatoprost release for up to 12 h, compared to free drug (p < 005). Most importantly, HET-CAM test nullified the irritancy of the formulation was verified its tolerability upon ocular use, as manifested by a significant reduction in mean irritation score, compared to positive control (1% sodium dodecyl sulfate; p < 0.001). Histopathology study inferred the absence of any signs of cornea tissue damage upon treatment with Bimatoprost optimized formulation. Collectively, it was concluded that SLNs might represent a viable vehicle for enhancing the corneal permeation and ocular bioavailability of Bimatoprost for the management of glaucoma.
The Effect of Additional Treatment with Empagliflozin or Semaglutide on Endothelial Function and Arterial Stiffness in Subjects with Type 1 Diabetes Mellitus—ENDIS Study
Authors: Navodnik Maja Preložnik , Janež Andrej , Žuran Ivan
Published: July 2023

We investigated the effect of additional treatment with newer antidiabetic drugs on endothelium function and arterial stiffness in subjects with type 1 diabetes mellitus (T1DM) without cardiovascular diseases. A total of 89 participants, all users of CGMS (continuous monitoring glucose system), were randomized into three comparable groups, receiving empagliflozin (E; n = 30), receiving semaglutide (S; n = 30), and a control group (C; n = 29). At baseline and 12 weeks post treatment, we measured FMD (brachial artery flow-mediated dilation) and FBF (forearm blood flow as reactive hyperemia assessed with strain gauge plethysmography) as parameters of endothelial function, as well as pulse wave velocity (PWV) and peripheral resistance as parameters of arterial stiffness. Improvement in FMD was significant in both intervention groups compared to controls (E group 2.0-fold, p = 0.000 and S group 1.9-fold, p = 0.000), with no changes between those two groups (p = 0.745). During the evaluation of FBF, there were statistically insignificant improvements in both therapeutic groups compared to controls (E group 1.39-fold, p = 0.074 and S group 1.22-fold, p = 0.701). In arterial stiffness parameters, improvements were seen only in the semaglutide group, with a decline in peripheral resistance by 5.1% (p = 0.046). We can conclude that, for arterial stiffness, semaglutide seems better, but both drugs positively impact endothelial function and, thus, could also have a protective role in T1DM.
Iron Oxide Nanoparticle-Mediated mRNA Delivery to Hard-to-Transfect Cancer Cells
Authors: Huang Jianxi , Lin Guanyou , Juenke Taylor , Chung Seokhwan , Lai Nicholas , Zhang Tianxin , Zhang Tianyi , Zhang Miqin
Published: July 2023

mRNA-based therapeutics have emerged as a promising strategy for cancer treatment. However, the effective delivery of mRNA into hard-to-transfect cancer cells remains a significant challenge. This study introduces a novel approach that utilizes iron oxide nanoparticles (NPs) synthesized through a layer-by-layer (LbL) method for safe and efficient mRNA delivery. The developed NPs consist of an iron oxide core modified with a thin charge-bearing layer, an mRNA middle layer, and an outer layer composed of perfluorinated polyethyleneimine with heparin (PPH), which facilitates efficient mRNA delivery. Through a comparative analysis of four nanoparticle delivery formulations, we investigated the effects of the iron oxide core’s surface chemistry and surface charge on mRNA complexation, cellular uptake, and mRNA release. We identified an optimal and effective mRNA delivery platform, namely, (IOCCP)-mRNA-PPH, capable of transporting mRNA into various hard-to-transfect cancer cell lines in vitro. The (IOCCP)-mRNA-PPH formulation demonstrated significant enhancements in cellular internalization of mRNA, facilitated endosomal escape, enabled easy mRNA release, and exhibited minimal cytotoxicity. These findings suggest that (IOCCP)-mRNA-PPH holds great promise as a solution for mRNA therapy against hard-to-transfect cancers.