bluuebunny/crossref_metadata_2025 huggingface dataset:
Critically Appraised Topic on Adverse Food Reactions of Companion Animals (8): Storage Mites in Commercial Pet foods
Authors: Olivry Thierry , Mueller Ralf S.
Published: October 2019

Background

Dogs with year-round atopic dermatitis are often sensitized to Dermatophagoides house dust mites (HDM). Storage mites (SM) are known to grow on cereal-rich foods. Tyrophagus SM can exacerbate clinical signs of allergy in laboratory dogs sensitized to HDM. Consequently, atopic dogs with high-levels of HDM-specific IgE are likely to have a flare of signs after eating a food contaminated with SM; the development of such flares would lead to a false positive diagnosis of food allergy. Herein, we reviewed the published evidence about the growth of SM on commercial dry pet foods.

Results

We searched two databases on January 25, 2019 for articles providing original information on the growth of SM on commercial dog foods. We found ten articles, five reporting results of laboratory experiments and five from field studies. Storage mites, especially Tyrophagus putrescentiae, can multiply on protein- and fat-rich dog foods. The population growth is higher when the initial mite density is high and when kibbles are crushed. When storage conditions lead to the overgrowth of molds on the kibbles, the mite proliferation is higher. Storage mites do not bore holes in food packages but invade bags via defective seals. In the field, SM contamination usually is undetectable in newly-opened commercial dog foods, and, if present, their number is low. When newly-purchased bags are stored in temperate conditions indoors, little overgrowth—if any—of SM occurs. However, when kept in environmental conditions with higher temperature and humidity, Tyrophagus mites will enter and proliferate in sealed food packages.

Conclusions

Commercial dry pet foods should be kept indoors and sealed to decrease the risk of contamination with SM. When performing dietary restriction (elimination) and provocation trials for the diagnosis of food allergies in dogs, it seems preferable to choose newly-purchased bags—of both original and testing diets—to reduce the probability of their contamination with SM, especially Tyrophagus putrescentiae. In case of doubt about the presence of SM in any of these foods, one should perform food challenges with single home-cooked ingredients. Storage mite contamination might lead to an erroneous diagnosis of food allergy in HDM-sensitized dogs.
The neuroprotective activity of heat-treated human platelet lysate biomaterials manufactured from outdated pathogen-reduced (amotosalen/UVA) platelet concentrates
Authors: Nebie Ouada , Devos David , Vingtdeux Valérie , Barro Lassina , Devedjian Jean-Christophe , Jonneaux Aurélie , Chou Ming-Li , Bordet Régis , Buée Luc , Knutson Folke , Blum David , Burnouf Thierry
Published: October 2019

Background

Effective neurorestorative therapies of neurodegenerative diseases must be developed. There is increasing interest in using human platelet lysates, rich in neurotrophic factors, as novel disease-modifying strategy of neurodegeneration. To ensure virus safety, pathogen reduction treatments should be incorporated in the preparation process of the platelet concentrates used as source material. We therefore investigated whether platelet concentrates (PC) pathogen-inactivated using a licensed photo-inactivation treatment combining photosensitive psoralen (amotosalen) and UVA irradiation (Intercept) can serve as source material to prepare platelet lysates with preserved neuroprotective activity in Parkinson’s disease models.

Methods

Intercept treated-PCs were centrifuged, when reaching expiry day (7 days after collection), to remove plasma and platelet additive solution. The platelet pellet was re-suspended and concentrated in phosphate buffer saline, subjected to 3 freeze-thaw cycles (− 80 °C/37 °C) then centrifuged to remove cell debris. The supernatant was recovered and further purified, or not, by heat-treatment as in our previous investigations. The content in proteins and neurotrophic factors was determined and the toxicity and neuroprotective activity of the platelet lysates towards LUHMES cells or primary cortical/hippocampal neurons were assessed using ELISA, flow cytometry, cell viability and cytotoxicity assays and proteins analysis by Western blot.

Results

Platelet lysates contained the expected level of total proteins (ca. 7–14 mg/mL) and neurotrophic factors. Virally inactivated and heat-treated platelet lysates did not exert detectable toxic effects on neither Lund human mesencephalic dopaminergic LUHMES cell line nor primary neurons. When used at doses of 5 and 0.5%, they enhanced the expression of tyrosine hydroxylase and neuron-specific enolase in LUHMES cells and did not significantly impact synaptic protein expression in primary neurons, respectively. Furthermore, virally-inactivated platelet lysates tested were found to exert very strong neuroprotection effects on both LUHMES and primary neurons exposed to erastin, an inducer of ferroptosis cell death.

Conclusion

Outdated Intercept pathogen-reduced platelet concentrates can be used to prepare safe and highly neuroprotective human heat-treated platelet pellet lysates. These data open reassuring perspectives in the possibility to develop an effective biotherapy using virally-inactivated platelet lysates rich in functional neurotrophins for neuroregenerative medicine, and for further bio-industrial development. However, the data should be confirmed in animal models.

Graphical abstract
Association of PCSK9 plasma levels with metabolic patterns and coronary atherosclerosis in patients with stable angina
Authors: Caselli Chiara , Del Turco Serena , Ragusa Rosetta , Lorenzoni Valentina , De Graaf Michiel , Basta Giuseppina , Scholte Arthur , De Caterina Raffaele , Neglia Danilo
Published: October 2019

Objective

Aim of this study was to evaluate the relationship of plasma PCSK9 with metabolic and inflammatory profile and coronary atherosclerotic burden in patients with suspected CAD enrolled in the EVINCI study.

Methods

PCSK9 was measured in 539 patients (60.3 ± 8.6 years, 256 males) with symptoms of CAD characterized by risk factors, bio-humoral profiles, and treatment. N = 412 patients underwent coronary computed tomography angiography (CTA) to assess the presence and characteristics of coronary atherosclerosis. A CTA score, combining extent, severity, composition, and location of plaques was computed.

Results

Patients were divided according to PCSK9 quartiles: I (< 136 ng/mL), II–III (136–266 ng/mL), and IV quartile (> 266 ng/mL). Compared with patients in quartile IV, patients in quartile I had a higher prevalence of the metabolic syndrome and higher values of body mass index. LDL- and HDL-cholesterol were significantly lower in patients in the quartile I than in those in quartile IV. Coronary CTA documented normal vessels in 30% and obstructive CAD in 35% of cases without differences among PCSK9 quartiles. Compared with patients with the highest levels, patients with the lowest PCSK9 levels had a higher CTA score mainly due to higher number of mixed non-obstructive coronary plaques. At multivariable analysis including clinical, medications, and lipid variables, PCSK9 was an independent predictor of the CTA score (coefficient − 0.129, SE 0.03, P < 0.0001), together with age, male gender, statins, interleukin-6, and leptin.

Conclusion

In patients with stable CAD, low PCSK9 plasma levels are associated with a particular metabolic phenotype (low HDL cholesterol, the metabolic syndrome, obesity, insulin resistance and diabetes) and diffuse non-obstructive coronary atherosclerosis.

Trial registration ClinicalTrials.gov NCT00979199. Registered September 17, 2009
The effect of concomitant fibromyalgia in HIV infected patients receiving antiretroviral therapy: a prospective cross-sectional study
Authors: Demirdal Umit Secil , Bilir Neriman , Demirdal Tuna
Published: October 2019

Background

HIV infected patients receiving antiretroviral therapy (ART) have extensive musculoskeletal system involvement. Arthralgia and myalgia are the most common forms. Fibromyalgia Syndrome (FMS) is a chronic pain syndrome of the musculoskeletal system characterized by diffuse pain including arthralgia and myalgia. These overlapping symptoms are suggested the relationship between HIV and FMS. The primary purpose of this study was to determine the prevalence of FMS in HIV/AIDS patients. The secondary objective was to investigate the effects of FMS on functional status, depression, fatigue, sleep pattern and quality of life.

Methods

A total of 225 HIV infected patients who were receiving ART were included in this cross-sectional prospective study. The demographic data of the participants, CD4 T-lymphocyte count (cells/mm3), viral load (> 40 copy/ml), and ART regimens were recorded. FMS diagnosis was based on 2016 revision of diagnostic criteria. All patients completed the following questionnaires: Fibromyalgia Impact Questionnaire (FIQ), Beck Depression Inventory (BDI), Pittsburgh Sleep Quality Index (PSQI), Fatigue Severity Scale (FSS), and SF-36 scale.

Results

FMS was found in 20% of the HIV infected patients (n = 45). The mean duration of disease was 4.74 ± 4.42 years; it was significantly longer in patients with FMS (p = 0.007). The median CD4 T-lymphocyte count was found to be 616.00 ± 303.91 cells/mm3, and it was significantly higher in patients without FMS (p = 0.06). No statistically significant difference was found between the two groups according to the drug regimens used. A statistically significant difference was found in FIQ, BDI, PSQI, FSS and all subgroups of the SF-36 scale between the patients with and without FMS (p = 0.001).

Conclusions

A slightly higher frequency of FMS was determined in HIV infected patients receiving ART compared to previous studies. It was shown that presence of FMS negatively affected the function, depression, fatigue, sleep, and quality of life. Detection of FMS may decrease depression, fatigue, and sleep disorders and increase the quality of life in HIV infected patients. FMS should be distinguished correctly for an accurate treatment management of HIV and for increasing ART compliance.
Cerium oxide nanoparticles improve liver regeneration after acetaminophen-induced liver injury and partial hepatectomy in rats
Authors: Córdoba-Jover Bernat , Arce-Cerezo Altamira , Ribera Jordi , Pauta Montse , Oró Denise , Casals Gregori , Fernández-Varo Guillermo , Casals Eudald , Puntes Victor , Jiménez Wladimiro , Morales-Ruiz Manuel
Published: October 2019

Background and aims

Cerium oxide nanoparticles are effective scavengers of reactive oxygen species and have been proposed as a treatment for oxidative stress-related diseases. Consequently, we aimed to investigate the effect of these nanoparticles on hepatic regeneration after liver injury by partial hepatectomy and acetaminophen overdose.

Methods

All the in vitro experiments were performed in HepG2 cells. For the acetaminophen and partial hepatectomy experimental models, male Wistar rats were divided into three groups: (1) nanoparticles group, which received 0.1 mg/kg cerium nanoparticles i.v. twice a week for 2 weeks before 1 g/kg acetaminophen treatment, (2) N-acetyl-cysteine group, which received 300 mg/kg of N-acetyl-cysteine i.p. 1 h after APAP treatment and (3) partial hepatectomy group, which received the same nanoparticles treatment before partial hepatectomy. Each group was matched with vehicle-controlled rats.

Results

In the partial hepatectomy model, rats treated with cerium oxide nanoparticles showed a significant increase in liver regeneration, compared with control rats. In the acetaminophen experimental model, nanoparticles and N-acetyl-cysteine treatments decreased early liver damage in hepatic tissue. However, only the effect of cerium oxide nanoparticles was associated with a significant increment in hepatocellular proliferation. This treatment also reduced stress markers and increased cell cycle progression in hepatocytes and the activation of the transcription factor NF-κB in vitro and in vivo.

Conclusions

Our results demonstrate that the nanomaterial cerium oxide, besides their known antioxidant capacities, can enhance hepatocellular proliferation in experimental models of liver regeneration and drug-induced hepatotoxicity.
Substituting prolonged sedentary time and cardiovascular risk in children and youth: a meta-analysis within the International Children’s Accelerometry database (ICAD)
Authors:   On behalf of the International Children’s Accelerometry Database (ICAD) Collaborators, Wijndaele Katrien , White Thomas , Andersen Lars Bo , Bugge Anna , Kolle Elin , Northstone Kate , Wedderkopp Niels , Ried-Larsen Mathias , Kriemler Susi , Page Angie S. , Puder Jardena J. , Reilly John J. , Sardinha Luis B. , van Sluijs Esther M. F. , Sharp Stephen J. , Brage Søren , Ekelund Ulf
Published: October 2019

Background

Evidence on the association between sitting for extended periods (i.e. prolonged sedentary time (PST)) and cardio-metabolic health is inconsistent in children. We aimed to estimate the differences in cardio-metabolic health associated with substituting PST with non-prolonged sedentary time (non-PST), light (LIPA) or moderate-to-vigorous physical activity (MVPA) in children.

Methods

Cross-sectional data from 14 studies (7 countries) in the International Children’s Accelerometry Database (ICAD, 1998–2009) was included. Accelerometry in 19,502 participants aged 3–18 years, together with covariate and outcome data, was pooled and harmonized. Iso-temporal substitution in linear regression models provided beta coefficients (95%CI) for substitution of 1 h/day PST (sedentary time accumulated in bouts > 15 min) with non-PST, LIPA or MVPA, for each study, which were meta-analysed.

Results

Modelling substitution of 1 h/day of PST with non-PST suggested reductions in standardized BMI, but estimates were > 7-fold greater for substitution with MVPA (− 0.44 (− 0.62; − 0.26) SD units). Only reallocation by MVPA was beneficial for waist circumference (− 3.07 (− 4.47; − 1.68) cm), systolic blood pressure (− 1.53 (− 2.42; − 0.65) mmHg) and clustered cardio-metabolic risk (− 0.18 (− 0.3; − 0.1) SD units). For HDL-cholesterol and diastolic blood pressure, substitution with LIPA was beneficial; however, substitution with MVPA showed 5-fold stronger effect estimates (HDL-cholesterol: 0.05 (0.01; 0.10) mmol/l); diastolic blood pressure: − 0.81 (− 1.38; − 0.24) mmHg).

Conclusions

Replacement of PST with MVPA may be the preferred scenario for behaviour change, given beneficial associations with a wide range of cardio-metabolic risk factors (including adiposity, HDL-cholesterol, blood pressure and clustered cardio-metabolic risk). Effect estimates are clinically relevant (e.g. an estimated reduction in waist circumference of ≈1.5 cm for 30 min/day replacement). Replacement with LIPA could be beneficial for some of these risk factors, however with substantially lower effect estimates.
Effect of Enterococcus faecalis 2001 on colitis and depressive-like behavior in dextran sulfate sodium-treated mice: involvement of the brain–gut axis
Authors: Takahashi Kohei , Nakagawasai Osamu , Nemoto Wataru , Odaira Takayo , Sakuma Wakana , Onogi Hiroshi , Nishijima Hiroaki , Furihata Ryuji , Nemoto Yukio , Iwasa Hiroyuki , Tan-No Koichi , Tadano Takeshi
Published: October 2019

Background

Patients with inflammatory bowel disease (IBD), including those with ulcerative colitis and Crohn’s disease, have higher rates of psychiatric disorders, such as depression and anxiety; however, the mechanism of psychiatric disorder development remains unclear. Mice with IBD induced by dextran sulfate sodium (DSS) in drinking water exhibit depressive-like behavior. The presence of Lactobacillus in the gut microbiota is associated with major depressive disorder. Therefore, we examined whether Enterococcus faecalis 2001 (EF-2001), a biogenic lactic acid bacterium, prevents DSS-induced depressive-like behavior and changes in peripheral symptoms.

Methods

We evaluated colon inflammation and used the tail suspension test to examine whether EF-2001 prevents IBD-like symptoms and depressive-like behavior in DSS-treated mice. The protein expression of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), X-linked inhibitor of apoptosis protein (XIAP), and cleaved caspase-3 in the rectum and hippocampus was assessed by western blotting. Hippocampal neurogenesis, altered nuclear factor-kappa B (NFκB) p65 morphometry, and the localization of activated NFκB p65 and XIAP were examined by immunohistochemistry.

Results

Treatment with 1.5% DSS for 7 days induced IBD-like pathology and depressive-like behavior, increased TNF-α and IL-6 expression in the rectum and hippocampus, activated caspase-3 in the hippocampus, and decreased hippocampal neurogenesis. Interestingly, these changes were reversed by 20-day administration of EF-2001. Further, EF-2001 administration enhanced NFκB p65 expression in the microglial cells and XIAP expression in the hippocampus of DSS-treated mice.

Conclusion

EF-2001 prevented IBD-like pathology and depressive-like behavior via decreased rectal and hippocampal inflammatory cytokines and facilitated the NFκB p65/XIAP pathway in the hippocampus. Our findings suggest a close relationship between IBD and depression.
Breastfeeding experiences and perspectives on support among Chinese mothers separated from their hospitalized preterm infants: a qualitative study
Authors: Yang Yuanyuan , Brandon Debra , Lu Hong , Cong Xiaomei
Published: November 2019

Background

Chinese mothers of preterm infants often face obstacles to breastfeeding and commonly experience prolonged maternal-infant separation when their high-risk infants are hospitalized in a Neonatal Intensive Care Unit (NICU). This separation hinders mother-infant attachment and the establishment of breastfeeding. Currently, little is known about Chinese mothers’ experiences breastfeeding their preterm infants, or their support needs. The aim of this study was to develop an understanding of mothers’ experiences breastfeeding a hospitalized preterm infant and the support needed to establish a milk supply during the period separation from their infants.

Methods

A qualitative descriptive study was conducted in Beijing in 2017. A total of 11 Chinese mothers were individually interviewed while separated from their infants. The interviews were audio-recorded and transcribed verbatim. A thematic analysis involving a seven-step protocol identified key themes.

Results

Mothers of preterm infants reported physically and mentally challenging breastfeeding experiences during the period they were separated from their babies. They viewed expressing breast milk as integral to their maternal role, even though some found expressing breastmilk exhausting. With little professional support available, the mothers depended upon nonprofessionals to establish breastfeeding.

Conclusions

The study identified the difficulties mothers experienced establishing a milk supply while separated from their preterm infants, and the importance of access to health professional support.
Image analysis-based recognition and quantification of grain number per panicle in rice
Authors: Wu Wei , Liu Tao , Zhou Ping , Yang Tianle , Li Chunyan , Zhong Xiaochun , Sun Chengming , Liu Shengping , Guo Wenshan
Published: October 2019

Background

The number grain per panicle of rice is an important phenotypic trait and a significant index for variety screening and cultivation management. The methods that are currently used to count the number of grains per panicle are manually conducted, making them labor intensive and time consuming. Existing image-based grain counting methods had difficulty in separating overlapped grains.

Results

In this study, we aimed to develop an image analysis-based method to quickly quantify the number of rice grains per panicle. We compared the counting accuracy of several methods among different image acquisition devices and multiple panicle shapes on both Indica and Japonica subspecies of rice. The linear regression model developed in this study had a grain counting accuracy greater than 96% and 97% for Japonica and Indica rice, respectively. Moreover, while the deep learning model that we used was more time consuming than the linear regression model, the average counting accuracy was greater than 99%.

Conclusions

We developed a rice grain counting method that accurately counts the number of grains on a detached panicle, and believe this method can be a huge asset for guiding the development of high throughput methods for counting the grain number per panicle in other crops.
Correction to: PHF8 upregulation contributes to autophagic degradation of E-cadherin, epithelial-mesenchymal transition and metastasis in hepatocellular carcinoma
Authors: Zhou Wuhua , Gong Li , Wu Qinchuan , Xing Chunyang , Wei Bajin , Chen Tianchi , Zhou Yuan , Yin Shengyong , Jiang Bin , Xie Haiyang , Zhou Lin , Zheng Shusen
Published: October 2019

In the publication of our publication [1], we have noticed there is a wrong label in Fig. 1e, in which the position of “HCC” and “Adjacent” should be transposed.
Saccharomyces cerevisiae exhibiting a modified route for uptake and catabolism of glycerol forms significant amounts of ethanol from this carbon source considered as ‘non-fermentable’
Authors: Aßkamp Maximilian R. , Klein Mathias , Nevoigt Elke
Published: October 2019

Background

Due to its inevitable formation during biodiesel production and its relatively high degree of reduction, glycerol is an attractive carbon source for microbial fermentation processes. However, glycerol is catabolized in a fully respiratory manner by the eukaryotic platform organism Saccharomyces cerevisiae. We previously engineered S. cerevisiae strains to favor fermentative metabolism of glycerol by replacing the native FAD-dependent glycerol catabolic pathway with the NAD-dependent ‘DHA pathway’. In addition, a heterologous aquaglyceroporin (Fps1 homolog) was expressed to facilitate glycerol uptake. The current study was launched to scrutinize the formation of S. cerevisiae’s natural fermentation product ethanol from glycerol caused by the conducted genetic modifications. This understanding is supposed to facilitate future engineering of this yeast for fermenting glycerol into valuable products more reduced than ethanol.

Results

A strain solely exhibiting the glycerol catabolic pathway replacement produced ethanol at concentrations close to the detection limit. The expression of the heterologous aquaglyceroporin caused significant ethanol production (8.5 g L−1 from 51.5 g L−1 glycerol consumed) in a strain catabolizing glycerol via the DHA pathway but not in the wild-type background. A reduction of oxygen availability in the shake flask cultures further increased the ethanol titer up to 15.7 g L−1 (from 45 g L−1 glycerol consumed).

Conclusion

The increased yield of cytosolic NADH caused by the glycerol catabolic pathway replacement seems to be a minimal requirement for the occurrence of alcoholic fermentation in S. cerevisiae growing in synthetic glycerol medium. The remarkable metabolic switch to ethanol formation in the DHA pathway strain with the heterologous aquaglyceroporin supports the assumption of a much stronger influx of glycerol accompanied by an increased rate of cytosolic NADH production via the DHA pathway. The fact that a reduction of oxygen supply increases ethanol production in DHA pathway strains is in line with the hypothesis that a major part of glycerol in normal shake flask cultures still enters the catabolism in a respiratory manner.
LIM and cysteine-rich domains 1 (LMCD1) regulates skeletal muscle hypertrophy, calcium handling, and force
Authors: Ferreira Duarte M. S. , Cheng Arthur J. , Agudelo Leandro Z. , Cervenka Igor , Chaillou Thomas , Correia Jorge C. , Porsmyr-Palmertz Margareta , Izadi Manizheh , Hansson Alicia , Martínez-Redondo Vicente , Valente-Silva Paula , Pettersson-Klein Amanda T. , Estall Jennifer L. , Robinson Matthew M. , Nair K. Sreekumaran , Lanner Johanna T. , Ruas Jorge L.
Published: October 2019

Background

Skeletal muscle mass and strength are crucial determinants of health. Muscle mass loss is associated with weakness, fatigue, and insulin resistance. In fact, it is predicted that controlling muscle atrophy can reduce morbidity and mortality associated with diseases such as cancer cachexia and sarcopenia.

Methods

We analyzed gene expression data from muscle of mice or human patients with diverse muscle pathologies and identified LMCD1 as a gene strongly associated with skeletal muscle function. We transiently expressed or silenced LMCD1 in mouse gastrocnemius muscle or in mouse primary muscle cells and determined muscle/cell size, targeted gene expression, kinase activity with kinase arrays, protein immunoblotting, and protein synthesis levels. To evaluate force, calcium handling, and fatigue, we transduced the flexor digitorum brevis muscle with a LMCD1-expressing adenovirus and measured specific force and sarcoplasmic reticulum Ca2+ release in individual fibers. Finally, to explore the relationship between LMCD1 and calcineurin, we ectopically expressed Lmcd1 in the gastrocnemius muscle and treated those mice with cyclosporine A (calcineurin inhibitor). In addition, we used a luciferase reporter construct containing the myoregulin gene promoter to confirm the role of a LMCD1-calcineurin-myoregulin axis in skeletal muscle mass control and calcium handling.

Results

Here, we identify LIM and cysteine-rich domains 1 (LMCD1) as a positive regulator of muscle mass, that increases muscle protein synthesis and fiber size. LMCD1 expression in vivo was sufficient to increase specific force with lower requirement for calcium handling and to reduce muscle fatigue. Conversely, silencing LMCD1 expression impairs calcium handling and force, and induces muscle fatigue without overt atrophy. The actions of LMCD1 were dependent on calcineurin, as its inhibition using cyclosporine A reverted the observed hypertrophic phenotype. Finally, we determined that LMCD1 represses the expression of myoregulin, a known negative regulator of muscle performance. Interestingly, we observed that skeletal muscle LMCD1 expression is reduced in patients with skeletal muscle disease.

Conclusions

Our gain- and loss-of-function studies show that LMCD1 controls protein synthesis, muscle fiber size, specific force, Ca2+ handling, and fatigue resistance. This work uncovers a novel role for LMCD1 in the regulation of skeletal muscle mass and function with potential therapeutic implications.
Abiotic environmental factors override phytoplankton succession in shaping both free-living and attached bacterial communities in a highland lake
Authors: Wang Huan , Zhu Rong , Zhang Xiaolin , Li Yun , Ni Leyi , Xie Ping , Shen Hong
Published: October 2019

Bacterial communities are an important part of biological diversity and biogeochemical cycling in aquatic ecosystems. In this study, the relationship amongst the phytoplankton species composition and abiotic environmental factors on seasonal changes in the community composition of free-living and attached bacteria in Lake Erhai were studied. Using Illumina high-throughput sequencing, we found that the impact of environmental factors on both the free-living and attached bacterial community composition was greater than that of the phytoplankton community, amongst which total phosphorus, Secchi disk, water temperature, dissolved oxygen and conductivity strongly influenced bacterial community composition. Microcystis blooms associated with subdominant Psephonema occurred during the summer and autumn, and Fragilaria, Melosira and Mougeotia were found at high densities in the other seasons. Only small numbers of algal species-specific bacteria, including Xanthomonadaceae (Proteobacteria) and Alcaligenaceae (Betaproteobacteria), were tightly coupled to Microcystis and Psephonema during Microcystis blooms. Redundancy analysis showed that although the composition of the bacterial communities was controlled by species composition mediated by changes in phytoplankton communities and abiotic environmental factors, the impact of the abiotic environment on both free-living and attached bacterial community compositions were greater than the impact of the phytoplankton community. These results suggest that the species composition of both free-living and attached bacterial communities are affected by abiotic environmental factors, even when under strong control by biotic factors, particularly dominant genera of Microcystis and Psephonema during algal blooms.
Effect of the inoculation of plant growth-promoting rhizobacteria on the photosynthetic characteristics of Sambucus williamsii Hance container seedlings under drought stress
Authors: Liu Fangchun , Ma Hailin , Peng Lin , Du Zhenyu , Ma Bingyao , Liu Xinghong
Published: October 2019

Plant growth-promoting rhizobacteria (PGPR) are beneficial bacteria that survive within the range of plant rhizosphere and can promote plant growth. The effects of PGPR in promoting plant growth, activating soil nutrients, reducing fertilizer application, and improving the resistance of plant inducible system have been widely investigated. However, few studies have investigated PGPR as elicitors of tolerance to abiotic stresses, especially drought stress. In this study, the effects of Acinetobacter calcoaceticus X128 on the photosynthetic rate (Pn), stomatal conductance (Gs), intracellular CO2 concentration (Ci), and total chlorophyll content [Chl(a+b)] of Sambucus williamsii Hance seedling leaves under moderate drought stress and drought-rewatering conditions were determined. Compared with those of uninoculated seedlings, the average Pn values during the entire drought stress of inoculated seedlings increased by 12.99%. As the drought duration was lengthened, Ci of uninoculated leaves continued to increase after rapidly declining, whereas Gs continuously decreased. Furthermore, their photosynthetic properties were simultaneously restricted by stomatal and non-stomatal factors. After X128 inoculation, Ci and Gs of S. williamsii Hance leaves continued to decrease, and their photosynthetic properties were mainly restricted by stomatal factors. At the end of the drought stress, water stress reduced [Chl(a + b)] of S. williamsii Hance leaves by 13.49%. However, X128 inoculation decreased this deficit to only 7.39%. After water supply was recovered, Pn, Gs, and [Chl(a+b)] in uninoculated leaves were reduced by 14.23%, 12.02%, and 5.86%, respectively, relative to those under well-watered conditions. However, Ci increased by 6.48%. Compared with those of uninoculated seedlings, Pn, Gs, and [Chl(a+b)] in X128-inoculated seedlings were increased by 9.83%, 9.30%, and 6.85%, respectively. Therefore, the inoculation of X128 under arid environments can mitigate the reduction of chlorophyll, delay the restriction caused by non-stomatal factors to Pn in plant leaves under water stress, and can be more conducive to the recovery of photosynthetic functions of leaves after water supply is recovered.
Application of biological and single-strand conformation polymorphism assays for characterizing potential mild isolates of Citrus tristeza virus for cross protection
Authors: Atta Sagheer , Umar Ummad ud din , Bashir Muhammad Amjad , Hannan Abdul , Rehman Ateeq ur , Naqvi Syed Atif Hasan , Zhou Changyong
Published: October 2019

Citrus tristeza virus(CTV) by killing millions of citrus cultivars grown on sour orange rootstock worldwide has become one of the most dangerous viral pathogen. Characterization of 12 CTV isolates was analyzed by biological indexing. Infected samples of citrus were collected from citrus growing areas of Pakistan and CTV was detected by symptoms on indicator plants and confirmed by direct tissue blot immunoassay (DTBIA). CTV positive samples were graft inoculated on six biological indicator hosts in the study. A standardized protocol was deployed to study biological characteristics of these isolates. All biological indicators induced mild and from mild to moderate reactions against all of the CTV isolates tested. About two isolates produced stem-pitting symptoms from moderate to severe on Mexican lime. CTV strains were further characterized and confirmed by the analysis of p25 gene of CTV isolates using single-strand conformation polymorphism (SSCP) assay. SSCP analysis revealed that most isolates confined only one predominant sequence variant. SSCP profiles of PCR amplified products from CTV isolates showed bands patterns corresponding to mild and sever strain. Three isolates (4MF, 8KBS and 10GS) from different regions and cultivars were identified as potential source of mild strains for cross protection. These results are the best base for mild strain cross protection (MSCP) in the country.
Assessing peer review pattern and the effect of face-to-face and mobile-mediated modes on students’ academic writing development
Authors: Farahani Ali Akbar Khomeijani , Nemati Majid , Nazari Montazer Mostafa
Published: October 2019

This study examines the distribution of peer review in face-to-face and mobile-mediated peer review groups and their effects on students’ revision skills and academic writing development. Seventy-two first-year English for academic purposes (EAP) students participated in an 18-session IELTS academic writing course in a Canadian university the mobile-mediated peer review group (MMPR) used Telegram to exchange peer comments synchronously, while the face-to-face peer review group (FFPR) did peer review in the classroom. An adapted analytic scheme (Journal of English for Academic Purposes, 2, 193–227, 2003) and the IELTS academic writing assessment criteria were used to conceptualize the peer comments in terms of frequency, area, type, nature, and IELTS assessment categories. Results indicated that the total number of comments, the percentage of revision-oriented comments and actual revisions made by the MMPR group were statistically more significant than those by the FFPR group. Furthermore, the MMPR group made more local revision-oriented comments than that of FFPR. However, the revision-oriented suggestion in local areas was the most distributed type of comment made by both groups. Regarding the IELTS assessment criteria, the FFPR group made more comments on task achievement and coherence and cohesion, whereas the comments made by the MMPR group targeted more lexical resources, and grammatical range and accuracy. In addition, the results showed that both MMPR and FFPR groups developed their IELTS academic writing skills while the MMPR mode of collaboration outperformed the FFPR.
Mendel’s pea crosses: varieties, traits and statistics
Authors: Ellis T. H. Noel , Hofer Julie M. I. , Swain Martin T. , van Dijk Peter J.
Published: October 2019

A controversy arose over Mendel’s pea crossing experiments after the statistician R.A. Fisher proposed how these may have been performed and criticised Mendel’s interpretation of his data. Here we re-examine Mendel’s experiments and investigate Fisher’s statistical criticisms of bias. We describe pea varieties available in Mendel’s time and show that these could readily provide all the material Mendel needed for his experiments; the characters he chose to follow were clearly described in catalogues at the time. The combination of character states available in these varieties, together with Eichling’s report of crosses Mendel performed, suggest that two of his F3 progeny test experiments may have involved the same F2 population, and therefore that these data should not be treated as independent variables in statistical analysis of Mendel’s data. A comprehensive re-examination of Mendel’s segregation ratios does not support previous suggestions that they differ remarkably from expectation. The χ2values for his segregation ratios sum to a value close to the expectation and there is no deficiency of extreme segregation ratios. Overall the χ values for Mendel’s segregation ratios deviate slightly from the standard normal distribution; this is probably because of the variance associated with phenotypic rather than genotypic ratios and because Mendel excluded some data sets with small numbers of progeny, where he noted the ratios “deviate not insignificantly” from expectation.
Ultra-high performance liquid chromatography-MS/MS (UHPLC-MS/MS) in practice: analysis of drugs and pharmaceutical formulations
Authors: Rathod Ravsaheb H. , Chaudhari Suraj R. , Patil Amod S. , Shirkhedkar Atul A.
Published: October 2019

Background

UHPLC-MS/MS is connected in various research facilities for the qualitative and quantitative investigation of a pharmaceutical substance, pharmaceutical items, and biological specimen.

Main body

The commence review article is an endeavor to offer pervasive awareness around assorted aspects and details about the UHPLC-MS/MS and related techniques with the aim on practice to an estimation of medicinal active agents in the last 10 years. The article also focused on isolation, separation, and characterization of present impurity in drug and biological samples.

Conclusion

Review article compiles a general overview of medicinally important drugs and their analysis with UHPLC-MS/MS. It gives fundamental thought regarding applications of UHPLC-MS/MS for the study on safety limit. The summary of developed UHPLC-MS/MS methods gives a contribution to the future trend and limitations in this area of research.
Frequency-dependent AVO analysis using the scattering response of a layered reservoir
Authors: Kumar Dhananjay , Zhao Zeyu , Foster Douglas J. , Dralus Danica , Sen Mrinal K.
Published: October 2019

Sensitivity of reservoir properties to broadband seismic amplitudes can be weak, which makes interpretation ambiguous. Examples of challenging interpretation scenarios include distinguishing blocky reservoirs from fining sequences, low gas saturation from high gas saturation, and variable reservoir quality. Some of these rock and fluid changes might indicate stronger sensitivity to amplitudes over narrow frequency bands, which is a characteristic of frequency-dependent amplitude variation with offset (FAVO). We have developed a FAVO model for reservoir characterization, following a seismic scattering phenomenon through a set of isotropic elastic layers. The frequency dependency in our model comes from the time delays due to wave propagation within layers. The FAVO modeled response is a complex-valued amplitude varying with angle and frequency, and it is a function of the seismic velocities and thicknesses of individual layers, along with the conventional AVO response at all interfaces. Our FAVO seismic analysis consists of two main steps: (1) forward modeling using well logs to understand rock and fluid sensitivity to amplitudes to identify tuning frequencies with maximum amplitude excursions and (2) seismic analysis at tuning frequencies. With well-log models, we observed that the frequency-dependent tuning response is primarily dependent on the lithology stacking pattern of a reservoir; in the cases studied, the fluid and reservoir quality have secondary effects on the frequency dependence of the amplitudes. We evaluate synthetic models and field data from the Columbus Basin, Trinidad, to illustrate our frequency-dependent seismic analysis methods. For one of the sandstone reservoirs, a frequency-dependent attribute indicates better spatial resolution of the anomaly than a conventional amplitude extraction. FAVO attributes are complementary to conventional AVO attributes.
Quantifying the acid-base status of dragonflies across their transition from breathing water to breathing air
Authors: Lee Daniel J. , Matthews Philip G. D.
Published: October 2019

Amphibiotic dragonflies show a significant increase in hemolymph total CO2 (TCO2) as they transition from water-breathing to air-breathing. This study examines the hemolymph acid-base status of dragonflies from two families (Aeshnidae and Libellulidae) as they transition from water to air. CO2 solubility (αCO2) and the apparent carbonic acid dissociation constant (pKapp) were determined in vitro, and pH/bicarbonate [HCO3−] plots were produced by equilibrating hemolymph samples with PCO2 between 0.5-5 kPa in custom-built rotating microtonometers. Hemolymph αCO2 varied little between families and across development (mean 0.355±0.005 mmol l−1 kPa−1) while the pKapp was between 6.23 to 6.27, similar to values determined for grasshopper hemolymph. However, the non-HCO3− buffer capacity for dragonfly hemolymph was uniformly low relative to other insects (3.6 to 5.4 mmol l−1 pH−1). While aeshnid dragonflies maintained this level as bimodally-breathing late-final instars and air-breathing adults, the buffer capacity of bimodally-breathing late-final instar Libellula nymphs increased substantially to 9.9 mmol l−1 pH−1. Using the pH/[HCO3−] plots and in vivo measurements of TCO2 and PCO2 from early-final instar nymphs, it was calculated that the in vivo hemolymph pH was 7.8 for an aeshnid nymph and 7.9 for a libellulid nymph, respectively. The pH/[HCO3−] plots show that the changes in acid-base status experienced by dragonflies across their development are more moderate than those seen in vertebrate amphibians. Whether these differences are due to dragonflies being secondarily aquatic, or arise from intrinsic differences between insect and vertebrate gas exchange and acid-base regulatory mechanisms, remains an open question.
Hearing in Caterpillars of the Monarch Butterfly (Danaus plexippus)
Authors: Taylor Chantel J. , Yack Jayne E.
Published: October 2019

Many species of caterpillars have been reported to respond to sound, but there has been limited formal study of what sounds they hear, how they hear them, and how they respond to them. Here we report on hearing in caterpillars of the Monarch butterfly (Danaus plexippus). Fourth and fifth instar caterpillars respond to sounds by freezing, contracting, and flicking their thorax in a vertical direction. Behavioural responses were evoked by sound frequencies between 50 and 900 Hz, with best sensitivity at 100-200 Hz. The lowest mean threshold was 79 dBSPL (particle velocity 605 µm/s) at 150 Hz. When presented with a repeated 200 Hz sound tone, caterpillars habituate by no longer responding. A series of ablation experiments confirmed that the primary sensory receptors are a pair of long hairs, called trichoid sensilla, located on the upper prothorax. These sensilla are ∼450 µm long, rest in a socket, and are innervated by a single bipolar sensory neuron. Removal of these setae reduced responses significantly compared to controls. Other setae contributed minimally to hearing in response to 200 Hz tones, and tubercles and prothoracic shields played no apparent role in sound reception. We propose that hearing functions to prevent attacks by aerial insect predators and parasitoids, which produce flight sounds in the frequency range of the caterpillars’ sensitivity. This research lays the foundation for further investigations on the function and evolution of hearing in caterpillars, and has significance for conservation of threatened monarch butterfly larvae living near noisy urban environments and roadways.
High concentrations of trimethylamines in slime glands inhibit skein unraveling in Pacific hagfish
Authors: Jain G. , Starksen M. , Singh K. , Hoang C. , Yancey P. , McCord C. , Fudge D.S.
Published: October 2019

Hagfish defend themselves from fish predators by producing large volumes of gill-clogging slime when they are attacked. The slime consists of seawater and two major components that are ejected from the slime glands: mucus and threads. The threads are produced within specialized cells and packaged into intricately coiled bundles called skeins. Skeins are kept from unraveling via a protein adhesive that dissolves when the skeins are ejected from the slime glands. Previous work revealed that hagfish slime glands have high concentrations of methylamines including trimethylamine N-oxide (TMAO), betaine (trimethylglycine), and dimethylglycine, however the function of these compounds in the slime glands is unknown. We hypothesized that methylamines have stabilizing effects on the skeins that prevent premature unraveling in the gland. To test this hypothesis, we quantified the effect of methylamines on skein unraveling in Pacific hagfish and found that TMAO and betaine have inhibitory effects on skein unraveling in vitro. Furthermore, we found that TMAO is a more effective inhibitor of unraveling than betaine, but the presence of TMAO synergistically boosts the inhibitory action of betaine. Glycine and dimethyl glycine were far less effective inhibitors of unraveling at natural concentrations. Our results support the hypothesis that high levels of trimethylamines in the slime glands may act to hold the coiled thread skeins together within gland thread cells, and they may do so by stabilizing adhesive proteins. These results advance our knowledge of skein stabilization and deployment and provide another example of trimethylamines functioning to stabilize proteins in a marine organism.
Validation of a 3D CBCT-based protocol for the follow-up of mandibular condyle remodeling
Authors: Verhelst Pieter-Jan , Shaheen Eman , de Faria Vasconcelos Karla , Van der Cruyssen Fréderic , Shujaat Sohaib , Coudyzer Walter , Salmon Benjamin , Swennen Gwen , Politis Constantinus , Jacobs Reinhilde
Published: November 2019

Objectives:

Three-dimensional models of mandibular condyles provide a way for condylar remodeling follow-up. The overall aim was to develop and validate a user-friendly workflow for cone beam CT (CBCT)-based semi-automatic condylar registration and segmentation.

Methods:

A rigid voxel-based registration (VBR) technique for registration of two post-operative CBCT-scans was tested. Two modified mandibular rami, with or without gonial angle, were investigated as the volume of interest for registration. Inter- and intraoperator reproducibility of this technique was tested on 10 mandibular rami of orthognathic patients by means of intraclass correlation coefficients (ICC’s) and descriptive statistics of the transformation values from the VBR. The difference in reproducibility between the two modified rami was evaluated using a paired t-test (p < 0.05). For the segmentation, eight fresh frozen cadaver heads were scanned with CBCT and micro-CT. These data were used to test the inter- and intraoperator reproducibility (ICC’s) and accuracy (Bland–Altman plot) of a newly designed workflow based on semi-automated contour enhancement.

Results:

Excellent ICC’s (0.94–0.99) were obtained for the voxel-based registration technique using both modified rami. If the gonial angle was not included in the volume of interest, there was a trend of increased operator error suggested by significant higher interoperator differences in translation values (p = 0,0036). The segmentation workflow proved to be highly reproducible with excellent ICC’s (0.99), low absolute mean volume differences between operators (23.19 mm3), within operators (28.93 mm3) and low surface distances between models of different operators (<0.20 mm). Regarding the accuracy, CBCT-models slightly overestimate the condylar volume compared to micro-CT.

Conclusions:

This study provides a validated user-friendly and reproducible method of creating three-dimensional-surface models of mandibular condyles out of longitudinal CBCT-scans.
Time to initiate randomized controlled clinical trials with methadone in cancer patients
Authors: Kremer Hans-Joachim
Published: October 2019

Public media coverage has fueled a demand for methadone as potential cure for cancer itself. Because patients have asked for respective prescriptions, clinical societies issued statements warning against the use of methadone as long as preclinical findings have not been supported by clinical evidence. In fact, not all preclinical data clearly support relevant effects. However, strong epidemiologic data suggest beneficial effects of methadone on cancer. Alternative explanations, namely better safety of methadone or hidden selection bias, seem less likely. This uncertainty can only be resolved by randomized controlled clinical trials. This review discusses all relevant data pertinent to methadone and cancer, uncovers supportive epidemiologic data, and suggests possible study designs.