bluuebunny/crossref_metadata_2025 huggingface dataset:
Uncommon Cold-Rolling Faults in an Fe–Mn–Si–Cr Shape-Memory Alloy
Authors: Bădărău Gheorghe , Popa Mihai , Stoian George , Roman Ana-Maria , Comăneci Radu-Ioachim , Pricop Bogdan , Cimpoeșu Nicanor , Bujoreanu Leandru-Gheorghe
Published: March 2024

The paper analyzes the occurrence of evenly spaced cracks on the surface of lamellar specimens of Fe-28Mn-6Si-5Cr (mass %) shape-memory alloy (SMA), during cold rolling. The specimens were hot rolled and normalized and developed cold rolling cracks with an approximate spacing of about 1.3 mm and a depth that increased with the thickness-reduction degree. At normalized specimens, X-ray diffraction patterns revealed the presence of multiple crystallographic variants of brittle α′ body-bcc martensite, which could be the cause of cold-rolling cracking. Both normalized and cold-rolled specimens were analyzed using scanning electron microscopy SEM. SEM micrographs revealed the presence of several crystallographic variants of α′-body-centered cubic (bcc) and ε hexagonal close-packed (hcp) martensite plates within a γ-face-centered cubic (fcc) austenite matrix in a normalized state. High-resolution SEM, recorded after 25% thickness reduction by cold-rolling, emphasized the ductile character of the cracks by means of an array of multiple dimples. After additional 33% cold-rolling thickness reduction, the surface of crack walls became acicular, thus revealing the fragile character of failure. It has been argued that the specimens cracked in the neutral point but preserved their integrity owing to the ductile character of γ-fcc austenite matrix.
Raman Spectroscopy of Crystalline Materials and Nanostructures
Authors: Nogueira Bernardo A. , Castiglioni Chiara
Published: March 2024

One of the biggest challenges in the field of material science lies in understanding the structure and behavior of crystalline materials and nanostructures [...]
The Changing Biogeography of the Ligurian Sea: Seawater Warming and Further Records of Southern Species
Authors: Azzola Annalisa , Bianchi Carlo Nike , Merotto Lorenzo , Nota Alessandro , Tiralongo Francesco , Morri Carla , Oprandi Alice
Published: March 2024

Global warming is causing poleward expansion of species ranges. Temperate seas, in particular, are undergoing a process known as ‘tropicalisation’, i.e., the combination of sea-water warming and establishment of southern species. The Ligurian Sea is one of the coldest sectors of the Mediterranean and has thus been characterized by a dearth of warm-temperate species and a comparative abundance of cold-temperate species. This paper uses a time series of sea surface temperature (SST) and new records of thermophilic fish species to reconsider the biogeography of the Ligurian Sea. SST has risen by about 0.7 °C on average between 1948 and 2023, but two phases may be distinguished: a cool one (ended in the mid-1980s) and a warm one (still ongoing); the latter phase shows alternating periods of rapid warming and comparatively stationary temperature. The arrival of thermophilic species coincided with the periods of rapid warming; some of these species were established in the subsequent stationary periods. Heatwaves and climate-related diseases associated with the periods of rapid warming have caused mass mortalities of autochthonous species. Our knowledge on the biogeography of the Ligurian Sea was established during the cool phase; the present situation, however, calls for re-defining the chorological spectrum of the Ligurian Sea biota.
Sebaceomas in a Muir–Torre-like Phenotype in a Patient with MUTYH-Associated Polyposis
Authors: Guarrera Julia , Prezzano James C. , Mannava Kathleen A.
Published: March 2024

This case report describes a case of a patient with MUTYH-associated polyposis (MAP), who presented with multiple sebaceomas in a Muir–Torre-like phenotype. MAP is caused by mutations in MUTYH, a base excision repair gene responsible for detecting and repairing the 8-oxo-G:A transversion caused by reactive oxygen species. MAP is associated with an increased risk of developing adenomatous polyps and colorectal cancer. Muir–Torre syndrome is a clinical phenotype of Lynch syndrome, which presents with multiple cutaneous sebaceous neoplasms. Lynch syndrome, like MAP, increases the likelihood of developing colorectal cancer but with a different pathogenesis and mode of inheritance. This case demonstrates that in a patient presenting with multiple sebaceous neoplasms, further workup and genetic testing may be indicated, not only for Muir–Torre and Lynch syndrome but also for MAP.
Comparing Causal Bayesian Networks Estimated from Data
Authors: Ma Sisi , Tourani Roshan
Published: March 2024

The knowledge of the causal mechanisms underlying one single system may not be sufficient to answer certain questions. One can gain additional insights from comparing and contrasting the causal mechanisms underlying multiple systems and uncovering consistent and distinct causal relationships. For example, discovering common molecular mechanisms among different diseases can lead to drug repurposing. The problem of comparing causal mechanisms among multiple systems is non-trivial, since the causal mechanisms are usually unknown and need to be estimated from data. If we estimate the causal mechanisms from data generated from different systems and directly compare them (the naive method), the result can be sub-optimal. This is especially true if the data generated by the different systems differ substantially with respect to their sample sizes. In this case, the quality of the estimated causal mechanisms for the different systems will differ, which can in turn affect the accuracy of the estimated similarities and differences among the systems via the naive method. To mitigate this problem, we introduced the bootstrap estimation and the equal sample size resampling estimation method for estimating the difference between causal networks. Both of these methods use resampling to assess the confidence of the estimation. We compared these methods with the naive method in a set of systematically simulated experimental conditions with a variety of network structures and sample sizes, and using different performance metrics. We also evaluated these methods on various real-world biomedical datasets covering a wide range of data designs.
Investigating the Effect of Gear Ratio in the Case of Joint Multi-Objective Optimization of Electric Motor and Gearbox
Authors: Istenes György , Polák József
Published: March 2024

In this paper, a software framework is presented through an application that is able to jointly optimize an electric motor and a gearbox for the design of a drive system for electric vehicles. The framework employs a global optimization method and uses both analytical and finite element method (FEM) models to evaluate the objective functions. The optimization process is supported by a statistical surrogate model, which allows a large reduction of runtime. An earlier version of this framework was only suitable for electric motor optimization. In the application presented in a previous paper, the motor of a belt-driven electric drive system was optimized. In this paper, the optimization of the same drive system is shown, but now with a combined optimization of a gear drive and motor. The objective functions of optimization are minimizing the total loss energy and the weight of the drive system. The optimization results are compared with previous results to demonstrate the further potential of joint optimization.
Production of Sustainable Adsorbents for CO2 Capture Applications from Food Biowastes
Authors: Rubiera Fernando , Córdoba Carlos , Pena Tamara , Plaza Marta G.
Published: March 2024

Traditional methods to develop biomass-based carbon adsorbents generally involve carbonization followed by chemical or physical activation. However, routes involving the hydrothermal treatment of biomass are receiving growing interest. In this work, two different strategies for the synthesis of sustainable CO2 adsorbents are compared, i.e., in situ ionic activation and hydrothermal treatment followed by activation with CO2. The latter is a green and simple procedure that does not require the addition of chemicals or acid-washing stages, and which leads to carbon adsorbents with relatively high CO2 adsorption capacity at low pressures, up to 0.64 mmol g−1 at 15 kPa and 50 °C, conditions relevant for postcombustion CO2 capture applications. On the other hand, in situ ionic activation can lead to carbon adsorbents with superior CO2 adsorption capacity in the aforementioned conditions, 0.78 mmol g−1, and with reduced cost and environmental impact compared to conventional chemical activation.
Three-Dimensional Heterogeneity of the Pore and Fracture Development and Acoustic Emission Response Characteristics of Coal Rocks in the Yunnan Laochang Block
Authors: Liu Xingzhi , Zhang Songhang , Xie Yongkang , Wang Tao
Published: March 2024

Studying the heterogeneity of coal reservoirs is significant to coal bed methane (CBM) exploitation. To investigate the development of the pore–fracture and acoustic emission response characteristics of the coal rock in the Yunnan Laochang block, four cores were extracted from the same coal rock in different directions. Through a comprehensive analysis using CT scanning and three-axis compression tests combined with synchronous acoustic emission experiments, a three-dimensional visualization of the pore–fracture structure and an analysis of the acoustic emission process during the elastic phase were conducted. Additionally, the impact of the heterogeneous development of pore–fractures on the acoustic emission characteristics was discussed. The results show that: there is strong heterogeneity in pore and fracture development within the coal rock, with the most significant development occurring along the direction of vertical stratification; the acoustic emission process in the elastic phase can be divided into three stages: strong–weak–strong; the development of pores and fractures affects the acoustic emission characteristics, with both counts and signal strength increasing as the percentage of voids rises; and the inferred in situ stress aligns with strike-slip faulting stress using acoustic emission. These results can provide a reference for the actual project.
Analysis of the Cooling Modes of the Lining of a Ferroalloy-Casting Ladle
Authors: Prikhodko Evgeniy , Nikiforov Alexandr , Kinzhibekova Akmaral , Aripova Nazgul , Karmanov Amangeldy , Ryndin Vladimir
Published: March 2024

An important element of the operation of high-temperature aggregates are modes that change over time. During these modes, maximum temperature changes are recorded in the cross-section of the lining of the aggregate. The difference in temperature leads to the formation of thermal stresses, which are the main reason for the repair of aggregates. During rapid heating, the inner layers of the lining are subjected to compressive stresses, while during rapid cooling, these layers experience tensile stresses. Under the same conditions, rapid cooling of the lining is more critical, since refractories have poor resistance to tension. The purpose of the study is to calculate and analyze the thermal stresses that arise during cooling of the casting ladle lining. The stresses are determined based on the calculation of the unsteady temperature field of the lining. Thermal stress values are necessary for analysis of the current cooling rates of casting ladles and subsequent development of optimal cooling modes for the lining. To solve the heat conductivity equation, a numerical method was chosen using an implicit four-point difference scheme. To study the cooling process of the casting ladle lining, temperature measurements were carried out in the zone of the greatest wear of the lining. Under conditions of natural convection, cooling of the casting ladle lining occurs unevenly. Cooling schedules during natural convection are characterized by significant unevenness and high rates of temperature decrease. The cooling rates of the inner surface of the lining at the initial stage of cooling significantly exceed the values recommended in the technical literature. Such cooling rates lead to the appearance of significant thermal stresses in the lining. For a refractory that has not been in service, the maximum thermal compressive stresses exceed the ultimate compressive strength by 1.27 times, and the tensile stresses exceed the corresponding limit values by 4.4 times. For refractories that have worked three fuses in the ladle lining, the maximum thermal compressive stresses exceed the ultimate compressive strength by 1.28 times, and the tensile stresses exceed the corresponding limit values by 3.19 times. The studied cooling modes for the casting ladle lining are unacceptable for operation. Cooling, taking into account the indicated rates, leads to the destruction of the lining material. To increase the resistance and duration of the working campaign of casting ladle linings, it is necessary to develop cooling modes for the lining at speeds at which the resulting thermal stresses do not exceed the strength of the refractory materials.
Deadwood-Dwelling Beetles (Coleoptera: Eucnemidae) in a Beech Reserve: A Case Study from the Czech Republic
Authors: Zumr Václav , Nakládal Oto , Remeš Jiří
Published: March 2024

The saproxylic beetles (deadwood-dependent) belong to frequently studied groups of forest insects. Eucnemidae is a rare and poorly studied saproxylic family with a hidden life strictly related to deadwood. We studied the family Eucnemidae in a beech reserve, using 59 window traps placed on standing deadwood (snags) and lying logs. A total of 348 specimens in eight species were recorded in two seasons. The identified species included one critically endangered species (CR): Hylis cariniceps; five endangered species (EN): H. olexai, H. foveicollis, Isorhipis melasoides, Eucnemis capucina, and Microrhagus lepidus; one new species found in Bohemia (a region of the Czech Republic): Clypeorhagus clypeatus; and one common species: Melasis buprestoides. Most species preferred lying logs, but E. capucina and M. buprestoides preferred snags. Species richness (q = 0) was higher on lying logs than on snags, and similarly, Shannon diversity (q = 1) was significantly higher on lying logs compared to snags. The species C. clypeorghagus, H. foveicollis, H. cariniceps, and M. lepides preferred moist lying logs, while M. buprestoides and E. capucina preferred drier snags with cavities. The results suggest that in beech forests, lying logs serve as a fundamental habitat for the existence of Eucnemids. This could be due to the more stable microclimatic conditions inside the lying deadwood. From this perspective, our study may help better understand the biology of hidden and understudied rare saproxylic Eucnemids.
Genome-Wide Identification and Expression Analysis of Salt-Tolerance-Associated NAC Family Genes in Cyclocarya paliurus
Authors: Yang Ziwei , An Yin , Ye Qian , Zhang Nannan , Liu Xin , He Fayin , Zeng Yue , Tang Ming , Yang Zhengting , Li Kun
Published: March 2024

Soil salinity affects approximately 20% of the world’s arable land, presenting a significant challenge for studying the mechanisms by which plants adapt to saline environments. Cyclocarya paliurus, an invaluable research model due to its ecological and medicinal significance, is primarily concentrated in central and southern China. Nevertheless, Cyclocarya paliurus faces challenges from environmental factors such as soil salinization, which adversely impacts its growth, subsequently affecting the yield and quality of its bioactive compounds. The NAC gene family, a critical group of plant-specific transcription factors, plays pivotal roles in responding to abiotic stresses. However, there has not yet been any studies on NAC genes under salt stress in Cyclocarya paliurus. In this study, we identified 132 NAC genes within the Cyclocarya paliurus genome. Our analysis of the conserved structures and gene organization revealed a high degree of conservation in the proteins of the CpNAC gene family. Cis-element analysis unveiled the participation of these genes in a variety of biological processes, including light responses, phytohormone responses, cell cycle responses, and abiotic stress responses. Under salt stress conditions, the expression of 35 CpNAC genes changed significantly, indicating a response to salt treatment. Furthermore, we provided additional evidence for the identification of the NAC gene family and revealed their potential positive regulatory role in signal transduction by conducting a transcriptional activation activity analysis of CpNAC132(D) and CpNAC040, which are homologous to Arabidopsis thaliana NAC062/91 and NAC103, respectively. This research not only advances our comprehension of the salt stress adaptation in Cyclocarya paliurus but also provides robust support for future investigations into plant responses to environmental stress and the cultivation of salt-tolerant crops.
Diversity of Culturable Yeasts Associated with the Technification Level in the Process of Mezcal Production in the State of Durango
Authors: Martínez-Estrada Sandra Consuelo , Narváez-Zapata José Alberto , Rodríguez-Herrera Raúl , Grijalva-Ávila Julio , Gurrola-Reyes José Natividad , Larralde-Corona Claudia Patricia , Chairez-Hernández Isaías
Published: March 2024

Durango State has the denomination of origin for the production of mezcal, which is made from Agave durangensis, mainly in an artisanal way; therefore, differences in the fermentation process affect the quality of the final product. The main objective of the present study was to evaluate the diversity of culturable yeasts involved in the artisanal and semi-technified process of mezcal production in the State of Durango. Three distilleries with different production processes were monitored at different fermentation stages (beginning, mid-fermentation, and end of fermentation) in the spring and summer seasons. A greater diversity was found in the distillery of Nombre de Dios in both the spring and summer production seasons (H’ = 1.464 and 1.332, respectively), since it maintains an artisanal production process. In contrast, the distillery of Durango, where a Saccharomyces cerevisiae commercial inoculum is used to start fermentation, presented low diversity indexes (H’ = 0.7903 and 0.6442) and only S. cerevisiae, Kluyveromyces marxianus, and, sporadically, Pichia manshurica were found. Results suggest that the yeast microbiota involved in mezcal fermentation during the different seasons is affected by the type of inoculum; changes include the presence of some species that were only identified during a specific season in alcoholic fermentation, such as Torulaspora delbrueckii and Pichia kluyveri.
Changes in the Serum Biochemical Indices and Intestinal Microbial Community of Rabbitfish (Siganus oramin) in Three Different Habitats
Authors: Yang Yukai , Lin Heizhao , Huang Xiaolin , Dong Hongbiao , Duan Yafei
Published: March 2024

The rabbitfish Siganus oramin is an important naturally caught and aquaculture fish species. Intestinal microbiota can affect the metabolism and immunity of fish, which is closely related to the habitat of the host. In this study, we collected the wild fry S. oramin from a natural sea area, and cultured them in outdoor and indoor ponds, respectively, and investigated the changes in serum biochemical indexes and intestinal microbial community in three different habitats. The results showed that compared with the wild population, the serum total protein content of the outdoor culture population increased significantly. The indoor culture population had significantly higher triglyceride and total cholesterol contents than that of the outdoor culture population. Additionally, the intestinal microbial richness indexes ACE and Chao1 of the cultured population were higher than those of the wild population, especially the indoor culture, but Shannon and Simpson had no obvious changes. The relative abundances of Firmicutes, Spirochaetae and Bacteroidetes increased in the outdoor culture population, but decreased in the indoor culture population; those of Proteobacteria and Cyanobacteria were completely the opposite. Some putative beneficial bacteria (Lactobacillus, Clostridium sensu stricto 1, and Intestinibacter) and harmful bacteria (Vibrio, Photobacterium, Desulfovibrio, Streptococcus) all decreased in the intestines of the cultured population when compared with the wild population, while Akkermansia and Faecalibacterium were enriched in the outdoor culture population. These results reveal that a change in habitat environment, whether an outdoor or an indoor pond, positively influenced the intestinal microbiota of the rabbitfish, which is beneficial to the healthy culture of the fish from the perspective of microbial community.
Farmed Chinese Perch (Siniperca chuatsi) Coinfected with Parasites and Oomycete Pathogens
Authors: Xu Xiandong , Zhang Yanping , Ding Liyun , Huang Jiangfeng , Zhou Zhiyong , Chen Wenjing
Published: March 2024

Farming Chinese perch in aquaculture systems with artificial diets is a new method and developing trend. This method of raising Chinese perch has led to outbreaks of new diseases. In 2022, a disease outbreak occurred among farmed Chinese perch fed an artificial diet in Jiangxi Province, China, during which 50% of the fish died. The clinical signs exhibited by the diseased Chinese perch included decreased physical vitality, anorexia, emaciation, and dorsal fin ulceration with white cysts and flocs. Coinfection with ciliate parasites and an oomycete was found. No pathogenic bacteria were isolated from the microbial test, and the viral detection results were negative. After morphological and 18S or 28S rDNA sequence clustering analysis, the parasites were identified as Epistylis wuhanensis and Zoothamnium sp., while the oomycete was identified as Achlya klebsiana. This article discusses the interaction between parasitic and oomycete coinfections in Chinese perch from the perspective of the characteristics of Chinese perch raised with an artificial diet. The relationships between parasites, oomycete, and fish were also briefly discussed. This is the first report of the coinfection of Chinese perch with Epistylis wuhanensis, Zoothamnium sp. and Achlya klebsiana.
Turbulent Channel Flow: Direct Numerical Simulation-Data-Driven Modeling
Authors: Liakopoulos Antonios , Palasis Apostolos
Published: March 2024

Data obtained using direct numerical simulations (DNS) of pressure-driven turbulent channel flow are studied in the range 180 ≤Reτ≤ 10,000. Reynolds number effects on the mean velocity profile (MVP) and second order statistics are analyzed with a view of finding logarithmic behavior in the overlap region or even further from the wall, well in the boundary layer’s outer region. The values of the von Kármán constant for the MVPs and the Townsend–Perry constants for the streamwise and spanwise fluctuation variances are determined for the Reynolds numbers considered. A data-driven model of the MVP, proposed and validated for zero pressure-gradient flow over a flat plate, is employed for pressure-driven channel flow by appropriately adjusting Coles’ strength of the wake function parameter, Π. There is excellent agreement between the analytic model predictions of MVP and the DNS-computed MVP as well as of the Reynolds shear stress profile. The skin friction coefficient Cf is calculated analytically. The agreement between the analytical model predictions and the DNS-based computed discrete values of Cf is excellent.
Existence and Uniqueness of Some Unconventional Fractional Sturm–Liouville Equations
Authors: Gholizadeh Zivlaei Leila , Mingarelli Angelo B.
Published: March 2024

In this paper, we provide existence and uniqueness results for the initial value problems associated with mixed Riemann–Liouville/Caputo differential equations in the real domain. We show that, under appropriate conditions in a fractional order, solutions are always square-integrable on the finite interval under consideration. The results are valid for equations that have sign-indefinite leading terms and measurable coefficients. Existence and uniqueness theorem results are also provided for two-point boundary value problems in a closed interval.
Anti-UV Microgel Based on Interfacial Polymerization to Decrease Skin Irritation of High Permeability UV Absorber Ethylhexyl Methoxycinnamate
Authors: Wang Wei , He Qi-Tong , Chen Yin-Feng , Wang Bai-Hui , Xu Wen-Ying , Liu Qing-Lei , Liu Hui-Min
Published: March 2024

Ethylhexyl methoxycinnamate (EHMC) is frequently employed as a photoprotective agent in sunscreen formulations. EHMC has been found to potentially contribute to health complications as a result of its propensity to produce irritation and permeate the skin. A microgel carrier, consisting of poly(ethylene glycol dimethacrylate) (pEDGMA), was synthesized using interfacial polymerization with the aim of reducing the irritation and penetration of EHMC. The thermogravimetric analysis (TGA) indicated that the EHMC content accounted for 75.72% of the total composition. Additionally, the scanning electron microscopy (SEM) images depicted the microgel as exhibiting a spherical morphology. In this study, the loading of EHMC was demonstrated through FTIR and contact angle tests. The UV resistance, penetration, and skin irritation of the EHMC-pEDGMA microgel were additionally assessed. The investigation revealed that the novel sunscreen compound, characterized by limited dermal absorption, had no irritant effects and offered sufficient protection against ultraviolet radiation.
Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analyses Provide Comprehensive Insight into Molecular Mechanisms of Flavonoid Metabolites Biosynthesis in Styphnolobium japonicum
Authors: Wu Miao , Zhang Yu , Guo Peng , Liu Huiyuan , Xia Linkui , Wang Mengyuan , Zeng Chuqi , Wang Hongwei , Shang Fude
Published: March 2024

Styphnolobium japonicum L. is a commonly consumed plant in China, known for its medicinal and nutritional benefits. This study focuses on the medicinal properties influenced by flavonoid metabolites, which vary during flower development. Utilizing full-length transcriptome sequencing on S. japonicum flowers, we observed changes in gene expression levels as the flowers progressed through growth stages. During stages S1 and S2, key genes related to flavonoid synthesis (PAL, 4CL, CHS, F3H, etc.) exhibited heightened expression. A weighted gene co-expression network analysis (WGCNA) identified regulatory genes (MYB, bHLH, WRKY) potentially involved in the regulatory network with flavonoid biosynthesis-related genes. Our findings propose a regulatory mechanism for flavonoid synthesis in S. japonicum flowers, elucidating the genetic underpinnings of this process. The identified candidate genes present opportunities for genetic enhancements in S. japonicum, offering insights into potential applications for improving its medicinal attributes.
New Observational Material about Seismic and Non-Seismic Tsunamis in Greece and Surrounding Areas from 1900 to 2023
Authors: Triantafyllou Ioanna , Papadopoulos Gerassimos A.
Published: March 2024

A new set of observations has been compiled for tsunamis occurring in Greece and in the surrounding areas from 1900 to 2023. A variety of information sources has been collected and examined, including scientific and press reports, books, eyewitness accounts, pictorial and video material, and tide-gauge records. New material was also collected during our field surveys in the islands of Cephalonia, Karpathos, and Kos. Our investigation included 26 distinct events and revealed several tsunamis. The majority of them have remained unknown so far in the tsunami community. Our compilation also included little-known events for which further documentation has been provided. Among others, of particular importance is the collection of new information about the well-known tsunami associated with the 9 February 1948 large earthquake in Karpathos Island as well as the unknown so far series of local but powerful tsunamis generated during the seismic crisis of very strong earthquakes that destroyed the Ionian islands during August 1953. The new observational material collected is significant for the enrichment of existing tsunami catalogs with positive implications for better understanding the tsunami generation mechanisms and the assessment of tsunami hazards and risks.
Association of the Combination of Moderate-to-Vigorous Physical Activity and Sleep Quality with Physical Frailty
Authors: Yokote Tsubasa , Yatsugi Harukaze , Chu Tianshu , Liu Xin , Wang Lefei , Kishimoto Hiro
Published: March 2024

Background: The association of the individual and combined effects of moderate-to-vigorous physical activity (MVPA) and sleep quality with physical frailty in community-dwelling older adults is still unknown. Subjects and Methods: A cross-sectional study was conducted with a sample of older adults who had not required nursing care or support services. Physical frailty was assessed using Liu’s definition based on Fried’s concept. MVPA was measured by a triaxial accelerometer, and individuals who met either moderate physical activity (MPA) for ≥300 min/week, vigorous physical activity (VPA) for ≥150 min/week, or both were defined as “MVA+”. “SLP+” was defined as a Pittsburgh Sleep Quality Index score of <5.5 points. Results: A total of 811 participants were included in the final analysis. After adjusting for the multivariable confounding factors, the odds ratios (ORs) and 95% confidence intervals (CIs) for physical pre-frailty and frailty in the MVA−SLP+ (OR, 2.56; 95%CI, 1.80–3.62) and the MVA−SLP− group (OR, 3.97; 95%CI, 2.33–6.74) were significantly higher compared with the MVA+SLP+ group. Conclusion: Community-dwelling older adults who did not meet the MVPA criteria, regardless of sleep quality, had a higher prevalence of physical frailty.
Inguinoscrotal Hernia, a Possible Cause of Rapidly Developing Fetal Scrotal Mass: Case Report and Literature Update
Authors: Montironi Ramona , Giannubilo Stefano Raffaele , Cappanera Irene , Battistoni Giovanna Irene , Mancinelli Romina , Ciavattini Andrea
Published: March 2024

Inguinoscrotal hernia is a common pediatric disease but a rare condition in the fetus. We present a case, from our institution, of fetal inguinoscrotal hernia with possible rapid development. In addition to our case, we present a literature update on fetal inguinoscrotal hernia in order to enhance the ability to recognize it from the other scrotal masses on ultrasound. Antenatal management, differential diagnosis and postnatal management are also discussed.
Exploring the Production of Secondary Metabolites from a Halophyte Tetragonia tetragonoides through Callus Culture
Authors: Lee Ka Youn , Nam Do-Hyeon , Jeon Yongsam , Park Sang Un , Cho Jongki , Gulandaz Md Ashrafuzzaman , Chung Sun-Ok , Lee Geung-Joo
Published: March 2024

Considering Korea’s gradual shift toward an aging society, consumer interest in compounds with physiological benefits, including antioxidant and anticancer effects, has surged. This study explored the potential of Tetragonia tetragonoides (Pall.) Kunze, commonly known as New Zealand Spinach (NZS), a halophyte with reported health benefits, including efficacy in treating gastrointestinal diseases, high blood pressure, diabetes, and obesity. This study also introduced a novel callus culture system for NZS, allowing for the rapid in vitro production of secondary metabolites. Optimal callus induction (100%) and biomass production (0.416 g) were achieved by adding 2.0 mg·L−1 6-BA (6-Benzylaminopurine) and 0.5 mg·L−1 2,4-D (2,4-Dichlorophenoxyacetic acid) among five auxin and cytokinin combinations. Two distinct callus types, TGC [TDZ (Thidiazuron)-supplemented Green Callus] and TNYC [TDZ + NAA (Naphthalene acetic acid)-supplemented Yellow Callus], were identified, each with unique characteristics. The calli showed total phenolic and flavonoid contents comparable to those of NZS leaves grown in the greenhouse. An expression analysis of six genes (CHS, CHI, F3H, F3′H, FLS, and DFR) involved in the kaempferol biosynthesis revealed an enhanced flavonoid biosynthesis-related gene expression in TGC, emphasizing its potential for compound production. GC-MS analysis identified distinct compound profiles in TGC and TNYC, with 2,3-butanediol and succinic acid being the predominant compounds among the nine and forty-four components, respectively. These calli offer a stable supply of functional compounds and present an environmentally sustainable solution. The derived callus culture system is anticipated to contribute to the development of healthy functional foods or pharmaceuticals from halophyte NZS.
Correction: Fabris et al. Exploring Multivariate Profiles of Psychological Distress and Empathy in Early Adolescent Victims, Bullies, and Bystanders Involved in Cyberbullying Episodes. Int. J. Environ. Res. Public Health 2022, 19, 9871
Authors: Fabris Matteo Angelo , Longobardi Claudio , Morese Rosalba , Marengo Davide
Published: March 2024

In the published publication [...]
Flexible Cotton Fabric-Based Ionizing Radiation Dosimeter for 2D Dose Distribution Measurements over a Wide Dose Range at High Dose Rates
Authors: Kozicki Marek , Sąsiadek-Andrzejczak Elżbieta , Wach Radosław , Maras Piotr
Published: March 2024

This work presents an ecological, flexible 2D radiochromic dosimeter for measuring ionizing radiation in the kilogray dose range. Cotton woven fabric made of cellulose was volume-modified with nitrotetrazolium blue chloride as a radiation-sensitive compound. Its features include a color change during exposure from yellowish to purple-brown and flexibility that allows it to adapt to various shapes. It was found that (i) the dose response is up to ~80 kGy, (ii) it is independent of the dose rate for 1.1–73.1 kGy/min, (iii) it can be measured in 2D using a flatbed scanner, (iv) the acquired images can be filtered using a mean filter, which improves its dose resolution, (v) the dose resolution is −0.07 to −0.4 kGy for ~0.6 to ~75.7 kGy for filtered images, and (vi) two linear dose subranges can be distinguished: ~0.6 to ~7.6 kGy and ~9.9 to ~62.0 kGy. The dosimeter combined with flatbed scanner reading and data processing using dedicated software packages constitutes a comprehensive system for measuring dose distributions for objects with complex shapes.
Neutrophils at the Crossroads: Unraveling the Multifaceted Role in the Tumor Microenvironment
Authors: Awasthi Deepika , Sarode Aditya
Published: March 2024

Over the past decade, research has prominently established neutrophils as key contributors to the intricate landscape of tumor immune biology. As polymorphonuclear granulocytes within the innate immune system, neutrophils play a pivotal and abundant role, constituting approximately ∼70% of all peripheral leukocytes in humans and ∼10–20% in mice. This substantial presence positions them as the frontline defense against potential threats. Equipped with a diverse array of mechanisms, including reactive oxygen species (ROS) generation, degranulation, phagocytosis, and the formation of neutrophil extracellular traps (NETs), neutrophils undeniably serve as indispensable components of the innate immune system. While these innate functions enable neutrophils to interact with adaptive immune cells such as T, B, and NK cells, influencing their functions, they also engage in dynamic interactions with rapidly dividing tumor cells. Consequently, neutrophils are emerging as crucial regulators in both pro- and anti-tumor immunity. This comprehensive review delves into recent research to illuminate the multifaceted roles of neutrophils. It explores their diverse functions within the tumor microenvironment, shedding light on their heterogeneity and their impact on tumor recruitment, progression, and modulation. Additionally, the review underscores their potential anti-tumoral capabilities. Finally, it provides valuable insights into clinical therapies targeting neutrophils, presenting a promising approach to leveraging innate immunity for enhanced cancer treatment.
Poly(Propylene Carbonate)-Based Biodegradable and Environment-Friendly Materials for Biomedical Applications
Authors: Wang Li , Li Yumin , Yang Jingde , Wu Qianqian , Liang Song , Liu Zhenning
Published: March 2024

Poly(propylene carbonate) (PPC) is an emerging “carbon fixation” polymer that holds the potential to become a “biomaterial of choice” in healthcare owing to its good biocompatibility, tunable biodegradability and safe degradation products. However, the commercialization and wide application of PPC as a biomedical material are still hindered by its narrow processing temperature range, poor mechanical properties and hydrophobic nature. Over recent decades, several physical, chemical and biological modifications of PPC have been achieved by introducing biocompatible polymers, inorganic ions or small molecules, which can endow PPC with better cytocompatibility and desirable biodegradability, and thus enable various applications. Indeed, a variety of PPC-based degradable materials have been used in medical applications including medical masks, surgical gowns, drug carriers, wound dressings, implants and scaffolds. In this review, the molecular structure, catalysts for synthesis, properties and modifications of PPC are discussed. Recent biomedical applications of PPC-based biomaterials are highlighted and summarized.
EsigPBP3 Was the Important Pheromone-Binding Protein to Recognize Male Pheromones and Key Eucalyptus Volatiles
Authors: Fu Hengfei , Xiao Guipeng , Yang Zhende , Hu Ping
Published: March 2024

Pheromone-binding proteins (PBPs) are specific odorant-binding proteins that can specifically recognize insect pheromones. Through transcriptional analysis of the antennae of adult Endoclita signifer, EsigPBP3 was discovered and identified, and EsigPBP3 was found to be highly expressed in the antennae of male moths. Based on the binding characteristics and ability of EsigPBP3, we can find the key ligands and binding site to consider as a target to control the key wood bore E. signifier. In this study, the fluorescence competitive binding assays (FCBA) showed that EsigPBP3 had a high binding affinity for seven key eucalyptus volatiles. Molecular docking analysis revealed that EsigPBP3 had the strongest binding affinity for the sexual pheromone component, (3E,7E)-4,7,11-trimethyl-1,3,7,10-dodecatetraene. Furthermore, same as the result of FCBA, the EsigPBP3 exhibited high binding affinities to key eucalyptus volatiles, eucalyptol, α-terpinene, (E)-beta-ocimene, (−)-β-pinene, and (−)-α-pinene, and PHE35, MET7, VAL10, PHE38, ILE52, and PHE118 are key sites. In summary, EsigPBP3 exhibits high binding affinity to male pheromones and key volatile compounds and the crucial binding sites PHE35, MET7, VAL10, PHE38, ILE52, and PHE118 can act as targets in the recognition of E. signifier pheromones.
BAP31 Promotes Angiogenesis via Galectin-3 Upregulation in Neuroblastoma
Authors: Namusamba Mwichie , Wu Yufei , Yang Jiaying , Zhang Qi , Wang Changli , Wang Tianyi , Wang Bing
Published: March 2024

Neuroblastoma (NB) is one of the highly vascularized childhood solid tumors, and understanding the molecular mechanisms underlying angiogenesis in NB is crucial for developing effective therapeutic strategies. B-cell receptor-associated protein 31 (BAP31) has been implicated in tumor progression, but its role in angiogenesis remains unexplored. This study investigated BAP31 modulation of pro-angiogenic factors in SH-SY5Y NB cells. Through protein overexpression, knockdown, antibody blocking, and quantification experiments, we demonstrated that overexpression of BAP31 led to increased levels of vascular endothelial growth factor A (VEGFA) and Galectin-3 (GAL-3), which are known to promote angiogenesis. Conditioned medium derived from BAP31-overexpressing neuroblastoma cells stimulated migration and tube formation in endothelial cells, indicating its pro-angiogenic properties. Also, we demonstrated that BAP31 enhances capillary tube formation by regulating hypoxia-inducible factor 1 alpha (HIF-1α) and its downstream target, GAL-3. Furthermore, GAL-3 downstream proteins, Jagged 1 and VEGF receptor 2 (VEGFR2), were up-regulated, and blocking GAL-3 partially inhibited the BAP31-induced tube formation. These findings suggest that BAP31 promotes angiogenesis in NB by modulating GAL-3 and VEGF signaling, thereby shaping the tumor microenvironment. This study provides novel insights into the pro-angiogenic role of BAP31 in NB.
A Comparison of Spatial and Phenotypic Immune Profiles of Pancreatic Ductal Adenocarcinoma and Its Precursor Lesions
Authors: Enzler Thomas , Shi Jiaqi , McGue Jake , Griffith Brian D. , Sun Lei , Sahai Vaibhav , Nathan Hari , Frankel Timothy L.
Published: March 2024

Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease with a 5-year survival rate of 12.5%. PDAC predominantly arises from non-cystic pancreatic intraepithelial neoplasia (PanIN) and cystic intraductal papillary mucinous neoplasm (IPMN). We used multiplex immunofluorescence and computational imaging technology to characterize, map, and compare the immune microenvironments (IMEs) of PDAC and its precursor lesions. We demonstrate that the IME of IPMN was abundantly infiltrated with CD8+ T cells and PD-L1-positive antigen-presenting cells (APCs), whereas the IME of PanIN contained fewer CD8+ T cells and fewer PD-L1-positive APCs but elevated numbers of immunosuppressive regulatory T cells (Tregs). Thus, immunosuppression in IPMN and PanIN seems to be mediated by different mechanisms. While immunosuppression in IPMN is facilitated by PD-L1 expression on APCs, Tregs seem to play a key role in PanIN. Our findings suggest potential immunotherapeutic interventions for high-risk precursor lesions, namely, targeting PD-1/PD-L1 in IPMN and CTLA-4-positive Tregs in PanIN to restore immunosurveillance and prevent progression to cancer. Tregs accumulate with malignant transformation, as observed in PDAC, and to a lesser extent in IPMN-associated PDAC (IAPA). High numbers of Tregs in the microenvironment of PDAC went along with a markedly decreased interaction between CD8+ T cells and cancerous epithelial cells (ECs), highlighting the importance of Tregs as key players in immunosuppression in PDAC. We found evidence that a defect in antigen presentation, further aggravated by PD-L1 expression on APC, may contribute to immunosuppression in IAPA, suggesting a role for PD-L1/PD-1 immune checkpoint inhibitors in the treatment of IAPA.
Extraribosomal Functions of Bacterial Ribosomal Proteins—An Update, 2023
Authors: Aseev Leonid V. , Koledinskaya Ludmila S. , Boni Irina V.
Published: March 2024

Ribosomal proteins (r-proteins) are abundant, highly conserved, and multifaceted cellular proteins in all domains of life. Most r-proteins have RNA-binding properties and can form protein–protein contacts. Bacterial r-proteins govern the co-transcriptional rRNA folding during ribosome assembly and participate in the formation of the ribosome functional sites, such as the mRNA-binding site, tRNA-binding sites, the peptidyl transferase center, and the protein exit tunnel. In addition to their primary role in a cell as integral components of the protein synthesis machinery, many r-proteins can function beyond the ribosome (the phenomenon known as moonlighting), acting either as individual regulatory proteins or in complexes with various cellular components. The extraribosomal activities of r-proteins have been studied over the decades. In the past decade, our understanding of r-protein functions has advanced significantly due to intensive studies on ribosomes and gene expression mechanisms not only in model bacteria like Escherichia coli or Bacillus subtilis but also in little-explored bacterial species from various phyla. The aim of this review is to update information on the multiple functions of r-proteins in bacteria.
The Role of Polyphenols in Modulating PON1 Activity Regarding Endothelial Dysfunction and Atherosclerosis
Authors: Sirca Teodora , Mureșan Mariana , Pallag Annamaria , Marian Eleonora , Jurca Tunde , Vicaș Laura , Tunduc Ioana , Manole Felicia , Ștefan Liana
Published: March 2024

The incidence and prevalence of cardiovascular diseases are still rising. The principal mechanism that drives them is atherosclerosis, an affection given by dyslipidemia and a pro-inflammatory state. Paraoxonase enzymes have a protective role due to their ability to contribute to antioxidant and anti-inflammatory pathways, especially paraoxonase 1 (PON1). PON1 binds with HDL (high-density lipoprotein), and high serum levels lead to a protective state against dyslipidemia, cardiovascular diseases, diabetes, stroke, nonalcoholic fatty liver disease, and many others. Modulating PON1 expression might be a treatment objective with significant results in limiting the prevalence of atherosclerosis. Lifestyle including diet and exercise can raise its levels, and some beneficial plants have been found to influence PON1 levels; therefore, more studies on herbal components are needed. Our purpose is to highlight the principal roles of Praoxonase 1, its implications in dyslipidemia, cardiovascular diseases, stroke, and other diseases, and to emphasize plants that can modulate PON1 expression, targeting the potential of some flavonoids that could be introduced as supplements in our diet and to validate the hypothesis that flavonoids have any effects regarding PON1 function.
Identification of Gene–Allele System Conferring Alkali-Tolerance at Seedling Stage in Northeast China Soybean Germplasm
Authors: Zong Chunmei , Zhao Jinming , Wang Yanping , Wang Lei , Chen Zaoye , Qi Yuxin , Bai Yanfeng , Li Wen , Wang Wubin , Ren Haixiang , Du Weiguang , Gai Junyi
Published: March 2024

Salinization of cultivated soils may result in either high salt levels or alkaline conditions, both of which stress crops and reduce performance. We sampled genotypes included in the Northeast China soybean germplasm population (NECSGP) to identify possible genes that affect tolerance to alkaline soil conditions. In this study, 361 soybean accessions collected in Northeast China were tested under 220 mM NaHCO3:Na2CO3 = 9:1 (pH = 9.8) to evaluate the alkali-tolerance (ATI) at the seedling stage in Mudanjiang, Heilongjiang, China. The restricted two-stage multi-locus model genome-wide association study (RTM-GWAS) with gene–allele sequences as markers (6503 GASMs) based on simplified genome resequencing (RAD-sequencing) was accomplished. From this analysis, 132 main effect candidate genes with 359 alleles and 35 Gene × Environment genes with 103 alleles were identified, explaining 90.93% and 2.80% of the seedling alkali-tolerance phenotypic variation, respectively. Genetic variability of ATI in NECSGP was observed primarily within subpopulations, especially in ecoregion B, from which 80% of ATI-tolerant accessions were screened out. The biological functions of 132 candidate genes were classified into eight functional categories (defense response, substance transport, regulation, metabolism-related, substance synthesis, biological process, plant development, and unknown function). From the ATI gene–allele system, six key genes–alleles were identified as starting points for further study on understanding the ATI gene network.