bluuebunny/crossref_metadata_2025 huggingface dataset:
СТРАТЕГІЧНЕ ПЛАНУВАННЯ ЯК ІНСТИТУЦІЙНИЙ ФЕТИШ У ПУБЛІЧНОМУ УПРАВЛІННІ УКРАЇНИ: МЕТОДОЛОГІЧНІ, ПРОФЕСІЙНІ Й ЕТИЧНІ АСПЕКТИ ПРОБЛЕМИ
Authors: БОРЩЕВСЬКИЙ Віктор , ВАСИЛИЦЯ Оксана , ЧЕХ Мирослава
Published: October 2022

Досліджуються основні причини та способи поширення інституційних фетишів у публічному управлінні України. Наводяться основні приклади негативного впливу інституційних фетишів на ефективність публічного управління. Основну увагу приділено сучасним аспектам фетишизації інституту стратегічного планування у сфері публічного управління. Показано негативні наслідки його поширення на різних щаблях владної ієрархії. Акцентовано увагу на етичних і професійних проблемах публічного управління, пов’язаних з перетворенням стратегічного планування на інституційний фетиш. Здійснено огляд літературних джерел, присвячених проблемам фетишизації сфери публічного управління загалом та розвитку стратегічного планування в Україні зокрема. Метою статті визначено обґрунтування пріоритетних напрямів і першочергових засобів запобігання фетишизації стратегічного планування в публічному управлінні України. До головних чинників фетишизації стратегічного планування в публічному управлінні України віднесено: інституційні прогалини у системі нормативно-правового забезпечення, організаційно-управлінські деформації, а також недостатню ефективність механізму міжсекторних взаємодій. Доведено, що фетишизація інституту стратегічного планування в публічному управлінні України негативно впливає на професійну та етичну діяльність працівників органів державної влади та місцевого самоврядування. Вказаний негатив також проєктується на функціонування інститутів громадянського суспільства. Передусім це стосується демотивації їх персоналу, посилення виявів опортуністичної поведінки та схильності до толерування корупції. Для недопущення перетворення стратегічного планування в публічному управлінні України на інституційний фетиш пропонується активізувати використання інструментів ненасильницького примусу, включаючи ігрові, цифрові та соціальні технології. Крім того, великого значення у цьому контексті набуває інформаційно-просвітницька робота з фахівцями публічної сфери та більш активне залучення інститутів громадянського суспільства до процесів розробки та виконання стратегічних планів на загальнодержавному, регіональному та місцевому рівнях.
Condições para a qualidade na passagem de plantão da enfermagem e seus reflexos na qualidade da assistência de saúde
Authors: Pereira Geysiane da Rosa , Oliveira Joana de Oliveira
Published: October 2022

A passagem de plantão é um método utilizado pela enfermagem para assegurar a continuidade da assistência prestada. Baseia-se em estratégias propostas, utilizadas como atividade fundamental para a organização do trabalho. O profissional enfermeiro na pretensão de se preparar para a assistência prestada, vem adotando, na sua prática, estratégias administrativas que facilitem a sistematização de suas ações voltadas para o planejamento. Desse modo, questiona-se: quais as condições ideais para a passagem de plantão, visando a qualidade na assistência? Considerando a presente questão, o objetivo deste estudo é compreender as condições ideais na passagem de plantão, com base na literatura científica. A metodologia escolhida foi a revisão sistemática com abordagem qualitativa. Os resultados indicam que o enfermeiro, o paciente e a família devem fazer parte do processo de troca de plantão, contribuindo com as anotações feitas pelo enfermeiro que passará o turno de trabalho. Conclui-se que as condições ideais para a passagem de plantão envolvem o enfermeiro, o paciente e a família e deve envolver a comunicação para que a assistência mantenha qualidade.
MODEL PENGEMBANGAN EKONOMI DAERAH PESISIR DAN PULAU-PULAU KECIL MELALUI PENDEKATAN INTEGRASI MARINE-TOURISM INDUSTRY DI DESA BARU DAN BUKU LIMAU KECAMATAN MANGGAR KABUPATEN BELITUNG TIMUR
Authors: Reniati Reniati , Sabri Fadillah , Adi Wahyu
Published: October 2022

Tujuan dari Program KKN-PPM dapat dibagi menjadi tiga yaitu : Pemberdayaan Masyarakat, peningkatan peran Perguruan Tinggi dan memberikan dukungan bagi Pemerintah Daerah. Kegiatan tersebut dalam rangka memecahkan isu-isu permasalahan yang dihadapi oleh wilayah pesisir dan pulau-pulau kecil (WP3K) yaitu : belum optimalnya penataan dan pemanfaatan ruang di WP3K, masih rendahnya kualitas SDM dalam pengelolaan WP3K, terbatasnya sarana dan prasarana, belum optimalnya pemberdayaan sumberdaya pesisir untuk pembangunan ekonomi, belum optimalnya pengelolaan potensi perikanan, belum optimalnya pengembangan potensi dan objek wisata bahari, serta pencemaran dan kerusakan lingkungan.

Adapun kegiatan yang dilaksanakan untuk memecahkan isu-isu permasalahan yang dihadapi oleh wilayah pesisir dan pulau-pulau kecil (WP3K) diantaranya Pembuatan Master Plan“Kampong Wisata”, pembuatan plang desa wisata bahari, memperbaiki sistem drainase dan sanitasi lingkungan di areal pemukiman, ceramah agama, sosialisasi rencana pengelolaan wilayah dan tata ruang pesisir dan pulau-pulau di Kecamatan Manggar, pelatihan pengelolaan fasilitas air bersih, melatih anak-anak nelayan usia sekolah seni tari campak, pelatihan bidang kewirausahaan bidang pengolahan hasil perikanan, mengadakan sosialisasi pelestarian trumbu karang, pelatihan kepemimpinan bagi generasi muda, sosialisasi strategi Belitung Timur sebagai destinasi wisata bahari dunia, penanaman rumpon, kursus Bahasa Inggris bagi anak-anak, upload video ke You Tube, pelatihan perubahan mindset masyarakat nelayan, pelatihan kualitas kesehatan dan keluarga berencana, sosialisasi ancaman perusakan laut dan penggunaan alat tangkap yang berbahaya, pelatihan pembuatan aksesoris berbahan baku kerang dan pembuatan buku.
IPTEKS BAGI MASYARAKAT (IBM) APLIKASI DAN PELATIHAN PENGOLAHAN UNTUK MEMPEROLEH AIR BERSIH SECARA SEDERHANA MELALUI UPAYA PEMBERDAYAAN MASYARAKAT PESISIR DI SUNGAILIAT, KABUPATEN BANGKA
Authors: Helmi Henny , Umroh Umroh , Sari Suci Puspita
Published: October 2022

Masyarakat di wilayah Kampung Nelayan I dan II, Pesisir Sungailiat dalam kehidupan sehari-harinya menggunakan air dari sumur gali yang diperoleh dari sumur gali berwarna kuning, berasa, berbau amis, menimbulkan noda coklat dan kekuning-kuningan pada pakaian putih.  Air dari sumur gali rumah tangga tersebut tidak sesuai dengan standar kualitas air bersih dan dapat mengganggu kesehatan mereka. Tujuan utama dalam kegiatan ini adalah memberi pelatihan penyaringan air keruh menjadi air bersih untuk lingkungan masyarakat yang mengalami krisis air bersih dengan metode saringan bertingkat sederhana dan membangun dua unit contoh instalasi pengolahan air. Pelaksanaan kegiatan in melibatkan dua mitra sebagai pendukung utama pelaksanaan pembuatan fasilitas air bersih, yaitu kelompok Nelayan I dan Nelayan II. Kegiatan pelaksanaan pelatihan penyaringan air keruh dari sumur gali menjadi air bersih dengan metode Saringan Bertingkat Sederhana di wilayah nelayan telah dilakukan bersama-sama dengan  masyarakat pesisir dan mahasiswa sehingga setelah pelatihan, ilmunya dapat diaplikasikan secara langsung. Dua unit contoh instalasi air telah dibangun di Kampung Nelayan I dan Nelayan II, sebagai contoh agar masyarakat nanti mampu membuat unit pengolahan air secara mandiri. Dengan adanya kegiatan ini menghasilkan metode sederhana, aman, murah dan berbahan alami untuk menghasilkan  air bersih yang dapat digunakan masyarakat pesisir. Dua unit bangunan instalasi air telah digunakan oleh masyarakat dan masyarakat juga sudah diberi pelatihan cara merawat dan memelihara bangunan filtrasi. Penyadaran masyarakat mengenai cara hidup sehat telah dilakukan untuk peningkatan kualitas hidup masyarakat Kampung Nelayan.
Habilidades gerenciales y planeación estratégica factores de competitividad en mipymes comerciales: Azogues – Ecuador
Authors: Mantilla Crespo Xavier Augusto , Solís Muñoz Juan Bautista
Published: October 2022

Introducción. A las micro, pequeñas y medianas empresas (MiPymes) que están registradas en Ecuador son las atribuye del crecimiento de la producción, la generación de puestos de empleo, la transformación digital y la eficacia productiva del país. Las MiPymes se encuentran involucradas en las actividades productivas de la economía. Objetivo. Determinar la asociación positiva entre las habilidades gerenciales, la planeación estratégica y la competitividad en las Mipymes comerciales de Azogues- Ecuador. Metodología. El abordaje metodológico se fundamentó con un enfoque cuantitativo, se aplicó el muestreo no probabilístico e intencional. La investigación es exploratoria, descriptiva y correlacional; además, es transversal o transeccional y no experimental. La población fue finita de 156 sujetos de investigación, se aplicó un cuestionario a un marco muestral que incluye a 112 Mipymes. Resultados. Se aplico la prueba de Kolmogorov-Smirnov para determinar la normalidad o no de los datos recopilados, se obtiene un p valor de 0.00 para las tres variables, lo cual indica que los datos corresponden a una distribución normal (p<0.05), para determinar la correlación de las mismas se aplicó la prueba de Pearson, en la variable independiente Prom_HabilidadesGerenciales se obtiene 0.587** con relación a la variable dependiente Prom_Competitividad con una Sig. Bilateral de 0.000 <0.05 del p-valor en consecuencia es estadísticamente significativa. La variable independiente Prom_PlanificaciónEstratégica muestra una correlación fuerte de 0.578** y una Sig. Bilateral de 0.000 < 0.05 del p-valor, es decir, existe correlación con la variable Prom_Competitividad y es estadísticamente significativa. Los resultados sugieren que existe una relación significativa y directa con un grado de relación importante. Conclusiones. Los hallazgos de esta investigación hacen notar que las habilidades gerenciales y la planificación estratégica inciden positivamente en la competitividad de las Mipymes.
Modelo de gestión tecnológica para asegurar la calidad de la información en la UAOME-UCACUE-Ecuador
Authors: Guevara Vizcaíno Claudio Fernando , Solís Muñoz Juan Bautista
Published: October 2022

Introducción: La tecnología permite la gestión y el dinamismo de las organizaciones y garantiza el adecuado manejo del bien intangible más valioso de las empresas que es la información. El estudio aborda la importancia de la estructura organizacional de las empresas en el área de tecnologías de la información y comunicación. Objetivo: Identificar si el modelo de gestión tecnológica de la Universidad Católica de Cuenca permite asegurar la calidad de la información en la Unidad Académica de Otras Modalidades de Estudio. Se analiza la estructura de la Jefatura de TI- su arquetipo- así como el modelo de Gobierno y Gestión de TI frente a las necesidades de la Unidad Académica de Otras Modalidades de Estudio. Metodología: Se elaboró un estudio de campo con enfoque cualitativo de alcance descriptivo-exploratorio. Para la recolección de la información se aplicó un instrumento de 18 ítems alineados a las variables de estudio. Resultados: Con base en los constructos bilaterales se halla que las políticas y gestión digital están fuertemente correlacionadas con el Gobierno de TI 0.897**. La disponibilidad de información está linealmente asociada a la Gestión TI 0.808**. La calidad de la información está correlacionada con el modelo de Gestión Tecnológica en 0.895**. Los resultados son estadísticamente significativos a un nivel menor a 0.05 p-valor. Discusión: El esquema organizacional del área de TI incide en la calidad de los servicios de tecnología que brindan a las Unidades Académicas de la Universidad, esto permite garantizar la calidad y accesibilidad de la información para su uso en los diferentes procesos institucionales. Conclusión: Se concluyó que un modelo de gestión tecnológica basado en buenas prácticas, garantiza el adecuado manejo de la información, la calidad e integridad de la misma, y permite alinear los procesos de TI con los propósitos institucionales.
Occupational Pathology Service of the FMBA of Russia in 2021
Authors:   A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia, Bushmanov A.U. , Kasymova O.A. ,   A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia, Kretov A.S. ,   A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia, Tukov A.R. ,   A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia, Vlasova I.V. ,   A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia, Lomteva A.A. ,   A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia, Vunova A.A. ,   A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia
Published: October 2022

The Occupational Pathology Service of the FMBA of Russia in 2021 is represented by medical organizations involved in conducting preliminary and periodic medical examinations, and 17 centers of occupational pathology. The chief freelance professional pathologist annually collects reports on the results of the centers of occupational pathology. In accordance with the data presented, all the centers of occupational pathology of the FMBA of Russia have been established on the basis of multidisciplinary specialized healthcare institutions. According to the data of the Centers of Occupational Pathology of the FMBA of Russia, the most common causes of medical contraindications are diseases of the eye and its accessory apparatus, diseases of the circulatory system, diseases of the blood, hematopoietic organs and individual disorders involving the immune mechanism, diseases of the ear and mastoid process, which necessitates the implementation of preventive programs in the indicated directions. One of the main functions of the center for occupational pathology is the examination of the connection of the disease with the profession and professional suitability, including in complex and conflict cases. A specialized medical commission has been established to conduct these examinations in all centers of professional pathology of the FMBA of Russia. During 2021, these medical commissions considered 11436 expert cases. Since 2011, an Industry register of persons with occupational diseases has been maintained on the basis of the A.I. Burnazyan FMBC of the FMBA of Russia, the volume of which currently exceeds 2,300 cases. The system of psychophysiological examination of the FMBA of Russia in the structure of the occupational pathology service has 35 medical organizations, on the basis of which 43781 psychophysiological examinations were conducted in 2021.
Study on the sedentary lifestyle of adolescents in Khulna city
Authors: Paroi Silvia , Hasanat Md Abul , Roy Biplab Kumar
Published: October 2022

Objective: This cross-sectional study was carried out among the students of Rev. Paul’s High School, Gallamari, Khulna to assess the adolescents' sedentary lifestyle and also the related factors.
Methods: The sample size was 217 which were selected by systematic sampling method. Data were collected using a semi-structured questionnaire by a face-to-face interview conducted by the interviewer.
Results: In this study, it was found that only 43.3% of respondents had sedentary habits (watching TV, internet browsing, playing video games and not liking to go outside for playing in the field). Comparatively majority of girls (57.8%) were associated with sedentary habits. Otherwise whose mothers are educated were not associated with sedentary habits (62.9%). In this study, it was observed that among those who like fast foods, the majority had sedentary habits (24.5%). Also, about 64.3% of respondents who have sedentary habits were unwilling to perform outdoor physical activities like playing.
Conclusion: The findings of the result might help develop awareness among adolescents as well as parents and teachers regarding the sedentary lifestyle to reduce the risk of adverse health outcomes as well as to know about the associated factors. Thus, it will be helpful to minimize the factors from the daily lifestyle and become healthy citizens.
Mediscope 2022;9(2): 68-74
Analisa Balok Kantilever Dengan Beban Tidak Terbagi Merata
Authors: Dayera Disabella , Palungan Musa Bondaris , Ohello Febrian
Published: October 2022

Penelitian ini dilakukan untuk menyelidiki pengaruh beban yang terbagi secara merata terhadap deformasi yang terjadi pada sebuah balok kantiliver dengan melihat nilai slope dan defleksinya pada dua jenis material yaitu baja dan alumunium. Metode  penelitian  yang  digunakan  dalam penelitian ini yaitu metode integral ganda. Adapun hasil penelitian terhadap balok kantilever (baja dan aluminium) yang diberi beban terbagi secara merata yaitu untuk beban yang sama (6 N/m), perputaran sudut material baja lebih kecil dari perputaran sudut Alumunium , sehingga memperlihatkan hubungan slope dan beban yang diberikan  bersifat linier. Sedangkan, untuk nilai defleksi pada beban yang sama (6 N/m), nilai defleksi material Alumunium (0,82 mm) lebih besar dari defleksi atau lendutan material baja (0,29 mm), disebabkan karena elestitsitas material Alumunium (EAl = 70 GPa) lebih kecil dari elastisitas baja (ESt = 200 GPa).
Pendampingan Pemulihan Sosial Ekonomi Pasca Bencana Selama Pandemi Covid-19 di Desa Pemakuan, Kabupaten Banjar
Authors: Arista Abdul Irvan , Rahmattullah Muhammad , Aziza Naila , Fitri Annisa Norhayati , Ajijah Aulia , Rizki Auliana , Harianti  , Leni  , Aripin Muhammad Noor , Aulia Rabiatul , Putri Sheila Haifa , Hasanah Mahmudah
Published: October 2022

Di tengah masa pandemi Covid-19, Desa Pemakuan, Kabupaten Banjar yang terkenal sebagai Kampung Sagu beberapa waktu lalu tertimpa bencana alam yaitu banjir besar yang membuat masyarakat disana menderita kerugian karena tidak bisa menjalankan aktivitasnya dengan baik selama kurang lebih dua bulan. Tujuan pengabdian ini adalah untuk memberikan pendampingan dalam pemulihan sosial ekonomi pasca bencana di Desa Pemakuan, Kabupaten Banjar, Kecamatan Sungai Tabuk, Kabupaten Banjar. Lingkup kegiatan pengabdian ini meliputi pemulihan sosial ekonomi pasca bencana di desa tersebut. Metode pengabdian yang digunakan adalah pelatihan dan diskusi pada berbagai kegiatan bidang sosial ekonomi yang dilaksanakan selama 45 hari mulai tanggal 3 Juli 2021 sampai dengan 18 Agustus 2021, bertempat di desa Pemakuan, Kabupaten Banjar bersama dosen dan mahasiswa Program Studi Pendidikan Ekonomi FKIP Universitas Lambung Mangkurat dan masyarakat sekitar. Hasil dari pengabdian ini adalah terlaksananya berbagai pendampingan terkait pemulihan sosial ekonomi pasca bencana, mencakup: a) pelatihan pemasaran produk sagu, b) kegiatan mengajar SD Negeri Pemakuan, Kabupaten Banjar, c) program menciptakan pojok literasi untuk anak-anak di desa Pemakuan, Kabupaten Banjar, d) program membantu mengajar TPA, e) membantu administrasi desa Pemakuan, Kabupaten Banjar, serta f) program bakti sosial dan MJM (Malam Jumat Mengaji).
KELAPA “Kelompok Pengangkut Sampah” : Pemberdayaan Masyarakat (Ikatan Pemuda Aktif Mandiri) Sebagai Upaya Mewujudkan Lingkungan Yang Sehat
Authors: Muhidin Muh Azmi , Permana Lies , Agustini Rina Tri
Published: October 2022

Saah satu komponen penting dalam mewujudkan kesehatan masyarakat yakni kesehatan lingkungan. Namun seringkali ditemukan di masyarakat permasalahan lingkungan khususnya pengelolaan sampah yang belum optimal dalam penyelesaiannya. Fenomena serupa juga terjadi di  Kelurahan Sidodamai, Kecamatan Sungai Dama, Samarinda. Diketahui bahwa sebagian masyarakat jarang membuang sampah di TPS (Tempat Pembuangan Sampah), dikarenakan letak geografis lingkungan tempat tinggal yang kurang mendukung. Kondisi tersebut mengisyaratkan bahwa diperlukan upaya dan peran masyarakat untuk menangani masalah tersebut. Untuk itu dilakukan pemberdayaan masyarakat yang merupakan salah satu strategi promosi kesehatan untuk menggerakan dan memampukan masyarakat dalam mewujudkan lingkungan yang bersih dan sehat. Kegiatan pemberdayaan dilakukan dengan beberapa kegiatan di antaranya yaitu pembentukan divisi lingkungan pada organisasi IPAM (Ikatan Pemuda Aktif Mandiri), kemudian dilanjutkan dengan penentuan tim yang menjadi pengangkut sampah dan terakhir sosialisasi program kepada masyarakat. Kegiatan pemberdayaan tersebut diharapkan mampu mewujudkan lingkungan yang bersih dan sehat. Kelompok pengelola yang telah terbentuk juga diharapkan mampu mensosialisasikan dan menambah jumlah partisipasi masyarakat bergabung dalam pengangkutan sampah.
Sex Differences: Potential Impact in Vascular Physiopathology or Ischemic Heart Disease
Authors: Kingma Jr J.G.
Published: October 2022

Pathophysiological mechanisms for development and progression of ischemic heart disease, which is essentially an age-related condition, may differ between men and women. Age-related changes in cardiac and vascular anatomy and physiopathology combined with traditional risk factors affect the likelihood of developing heart disease. Sex specific features of cardiac and vascular remodeling with age are associated with a higher prevalence of ischemic heart disease in women post-menopause. Cardiac symptoms such as chest pain/discomfort are often less in women, which generally results in less aggressive clinical treatment compared to men. Incidentally, treatment guidelines for acute myocardial infarction have for the most part, been established from studies with a large male cohort. Sex differences relating to effects of ischemia on overall cardiovascular function could contribute to worse clinical outcomes for females. Treatment options for women also arise because they generally present with multiple co-morbidities. The present review focuses on recent findings for sex differences in pathogenesis of ischemic heart disease, vascular function as well as potential use of cardio protection strategies.
Soyanın Farklı Gelişim Dönemlerinde Uygulanan Yaprak Gübresinin Yaprak Alanı, Klorofil ve Besin İçeriklerine Etkisi
Authors: ŞAHİN Cenk Burak , İŞLER Necmi
Published: October 2022

Optimum büyümenin sağlanması ve yüksek verim için bitkiler, bitki besin elementlerinin toprakta alınabilir formda ve yeterince var olmasına gereksinim duymaktadır. Ancak, Akdeniz Bölgesi gibi kireç içeriği ve pH’sı yüksek topraklarda bulunan makro ve mikro besin elementlerinin bitkiler tarafından alımı kısıtlı olmakta, bazı durumlarda ise bitki tarafından kullanılamamaktadır. Bu gibi topraklarda gerekli besin elementinin, özellikle mikro bitki besin elementlerinin yaprak gübresi şeklinde verilmesi bitkinin daha hızlı ve etkili şekilde faydalanmasını sağlamaktadır. Bu çalışmada, soya bitkisinin (Glycine max L.) farklı gelişim dönemlerinde (V3, R1, R3) yaprak gübresi şeklinde verilen çinko ve demir besin elementlerinin klorofil içeriği (SPAD), yaprak alan indeksi (LAI) ve soya bitkisinin kimyasal kompozisyonuna etkisi incelenmiştir. Deneme, 2018 ve 2019 yıllarında ikinci ürün olarak Amik Ovası (Hatay) koşullarında bölünen bölünmüş parseller deneme desenine göre üç yinelemeli olarak yürütülmüştür. Ana parsellere çeşitler (Arısoy, Bravo, Nazlıcan), alt parsellere gelişim dönemleri (V3, R1, R3) ve alt alt parsellere gübre uygulamaları (Kontrol, Çinko, Demir, Çinko + Demir) yerleştirilmiştir. Soya bitkisine farklı gelişim dönemlerinde uygulanan demir ve çinko yaprak gübreleri, kontrol grubuna göre SPAD ve LAI değerlerini artırmıştır. En yüksek klorofil içeriği (SPAD değeri) Nazlıcan x R3 x Zn + Fe (36.03) uygulamasından alınırken, en yüksek yaprak alan indeksi değeri ise Bravo x V3 x Fe (7.57) uygulamasından alınmıştır. Klorofil içeriği tespitinde (SPAD değerleri) generatif dönem ölçümlerinde yüksek sonuç alınırken, yaprak alan indeksi ölçümlerinde ise vejetatif dönem daha iyi sonuç vermiştir. Yaprak besin elementi içerikleri yönünden incelendiğinde ise generatif dönemde yapılan uygulamalar neticesinde çinko ve demir birikimleri daha fazla olmuştur. Sonuç olarak, kireçli topraklarda soya bitkisinin ihtiyacı olan mikro besin elementlerinin yaprak gübrelemesi ile karşılanabileceği görülmüştür.
People’s Economic Development Model based on Micro, Small, and Medium Enterprises (MSMEs) during Covid19
Authors: Istiqomah Nurul , Mafruhah Izza , Noviani Leni , Akbar Leon , Ismoyowati Dewi
Published: October 2022

The aims of this study are analyzes the MSMEs business cycle based on the types of business in the COVID-19 pandemic, analyzes the supporting aspects in supporting the working system for MSMEs before and during the COVID-19 Pandemic, and analyzes MSMEs immunity. The findings of these three objectives will be used to formulate the MSME-based People's Economic Development Model. This study used a mixed method with a sequential explanatory model, where the researcher uses quantitative and qualitative methods sequentially. The quantitative method was carried out by analyzing the business cycle to determine the types of business according to the stages: peak, recession, depression or recovery so as to provide an overview of the pattern of MSME development. Result of this study are the MSMEs conditions during the COVID-19 pandemic can be divided into 4 quadrants: quadrant 1 Unable to market and unable to produce; quadrant 2, able produce but unable to market; quadrant 3, unable to produce but able to market; quadrant 4, able to produce and market. The role of stakeholders is divided into five actors, namely academics, business, government, society and mass media. The five actors together have their respective contributions to the development of MSMEs during the pandemic.Key words : MSME, Covid19, imunity, development model
SETH predicts nuances of residue disorder from protein embeddings
Authors: Ilzhöfer Dagmar , Heinzinger Michael , Rost Burkhard
Published: October 2022

Predictions for millions of protein three-dimensional structures are only a few clicks away since the release of AlphaFold2 results for UniProt. However, many proteins have so-called intrinsically disordered regions (IDRs) that do not adopt unique structures in isolation. These IDRs are associated with several diseases, including Alzheimer’s Disease. We showed that three recent disorder measures of AlphaFold2 predictions (pLDDT, “experimentally resolved” prediction and “relative solvent accessibility”) correlated to some extent with IDRs. However, expert methods predict IDRs more reliably by combining complex machine learning models with expert-crafted input features and evolutionary information from multiple sequence alignments (MSAs). MSAs are not always available, especially for IDRs, and are computationally expensive to generate, limiting the scalability of the associated tools. Here, we present the novel method SETH that predicts residue disorder from embeddings generated by the protein Language Model ProtT5, which explicitly only uses single sequences as input. Thereby, our method, relying on a relatively shallow convolutional neural network, outperformed much more complex solutions while being much faster, allowing to create predictions for the human proteome in about 1 hour on a consumer-grade PC with one NVIDIA GeForce RTX 3060. Trained on a continuous disorder scale (CheZOD scores), our method captured subtle variations in disorder, thereby providing important information beyond the binary classification of most methods. High performance paired with speed revealed that SETH’s nuanced disorder predictions for entire proteomes capture aspects of the evolution of organisms. Additionally, SETH could also be used to filter out regions or proteins with probable low-quality AlphaFold2 3D structures to prioritize running the compute-intensive predictions for large data sets. SETH is freely publicly available at: https://github.com/Rostlab/SETH.
Protein structure prediction in the era of AI: Challenges and limitations when applying to in silico force spectroscopy
Authors: Gomes Priscila S. F. C. , Gomes Diego E. B. , Bernardi Rafael C.
Published: October 2022

Mechanoactive proteins are essential for a myriad of physiological and pathological processes. Guided by the advances in single-molecule force spectroscopy (SMFS), we have reached a molecular-level understanding of how mechanoactive proteins sense and respond to mechanical forces. However, even SMFS has its limitations, including the lack of detailed structural information during force-loading experiments. That is where molecular dynamics (MD) methods shine, bringing atomistic details with femtosecond time-resolution. However, MD heavily relies on the availability of high-resolution structural data, which is not available for most proteins. For instance, the Protein Data Bank currently has 192K structures deposited, against 231M protein sequences available on Uniprot. But many are betting that this gap might become much smaller soon. Over the past year, the AI-based AlphaFold created a buzz on the structural biology field by being able to predict near-native protein folds from their sequences. For some, AlphaFold is causing the merge of structural biology with bioinformatics. Here, using an in silico SMFS approach pioneered by our group, we investigate how reliable AlphaFold structure predictions are to investigate mechanical properties of Staphylococcus bacteria adhesins proteins. Our results show that AlphaFold produce extremally reliable protein folds, but in many cases is unable to predict high-resolution protein complexes accurately. Nonetheless, the results show that AlphaFold can revolutionize the investigation of these proteins, particularly by allowing high-throughput scanning of protein structures. Meanwhile, we show that the AlphaFold results need to be validated and should not be employed blindly, with the risk of obtaining an erroneous protein mechanism.
Hydrogen sulfide activatable metal-organic frameworks for Fluorescence Imaging-Guided Photodynamic Therapy of colorectal cancer
Authors: Li Honghui , Huang Mao , Wei Zixuan , He Jiawen , Ma Yunong , Lu Cuixia , Jin Albert , Wang Zhixiong , Wen Liewei
Published: October 2022

Photodynamic therapy (PDT) is a promising alternative and palliative therapeutic strategy for colorectal cancer (CRC). A novel photosensitizer with higher selectivity for CRC and fewer side effects is vital for clinical application. Given that the overexpression of hydrogen sulfide (H2S) in CRC, it is expected to provide a selective stimulus for activatable photosensitizers that in respond to the specific microenvironment. Herein, we report a novel development of metal-organic frameworks (MOFs) composed of meso-Tetra (4-carboxyphenyl) porphine (TCPP) and ferric ion (Fe3+) through a facile one-pot process. Experiments both in vitro and in vivo reveal that the MOF is capable of depredating in response to the high content of H2S in tumor microenvironment of CRC. Accompanying with the degradation and release of TCPP, the fluorescence and photosensitivity effect is switched from “off” to “on”, enabling the MOF to serve as a H2S activatable nano-photosensitizer for real-time fluorescence imaging-guided and targeted PDT of CRC.
N-Net: Lesion region segmentations using the generalized hybrid dilated convolutions for polyps in colonoscopy images
Authors: Cui Rongsheng , Yang Runzhuo , Liu Feng , Cai Chunqian
Published: October 2022

Colorectal cancer is the cancer with the second highest and the third highest incidence rates for the female and the male, respectively. Colorectal polyps are potential prognostic indicators of colorectal cancer, and colonoscopy is the gold standard for the biopsy and the removal of colorectal polyps. In this scenario, one of the main concerns is to ensure the accuracy of lesion region identifications. However, the missing rate of polyps through manual observations in colonoscopy can reach 14%–30%. In this paper, we focus on the identifications of polyps in clinical colonoscopy images and propose a new N-shaped deep neural network (N-Net) structure to conduct the lesion region segmentations. The encoder-decoder framework is adopted in the N-Net structure and the DenseNet modules are implemented in the encoding path of the network. Moreover, we innovatively propose the strategy to design the generalized hybrid dilated convolution (GHDC), which enables flexible dilated rates and convolutional kernel sizes, to facilitate the transmission of the multi-scale information with the respective fields expanded. Based on the strategy of GHDC designing, we design four GHDC blocks to connect the encoding and the decoding paths. Through the experiments on two publicly available datasets on polyp segmentations of colonoscopy images: the Kvasir-SEG dataset and the CVC-ClinicDB dataset, the rationality and superiority of the proposed GHDC blocks and the proposed N-Net are verified. Through the comparative studies with the state-of-the-art methods, such as TransU-Net, DeepLabV3+ and CA-Net, we show that even with a small amount of network parameters, the N-Net outperforms with the Dice of 94.45%, the average symmetric surface distance (ASSD) of 0.38 pix and the mean intersection-over-union (mIoU) of 89.80% on the Kvasir-SEG dataset, and with the Dice of 97.03%, the ASSD of 0.16 pix and the mIoU of 94.35% on the CVC-ClinicDB dataset.
Recent advances in PCR-free nucleic acid detection for SARS-COV-2
Authors: Ma Xiaowei , Xu Jingsong , Zhou Fei , Ye Jing , Yang Donglei , Wang Hua , Wang Pengfei , Li Min
Published: October 2022

As the outbreak of Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory disease coronavirus 2 (SARS-COV-2), fast, accurate, and economic detection of viral infection has become crucial for stopping the spread. Polymerase chain reaction (PCR) of viral nucleic acids has been the gold standard method for SARS-COV-2 detection, which, however, generally requires sophisticated facilities and laboratory space, and is time consuming. This review presents recent advances in PCR-free nucleic acid detection methods for SARS-CoV-2, including emerging methods of isothermal amplification, nucleic acid enzymes, electrochemistry and CRISPR.
High-throughput optical action potential recordings in hiPSC-derived cardiomyocytes with a genetically encoded voltage indicator in the AAVS1 locus
Authors: Zhang Fangfang , Meier Anna B. , Poch Christine M. , Tian Qinghai , Engelhardt Stefan , Sinnecker Daniel , Lipp Peter , Laugwitz Karl-Ludwig , Moretti Alessandra , Dorn Tatjana
Published: October 2022

Cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) represent an excellent in vitro model in cardiovascular research. Changes in their action potential (AP) dynamics convey information that is essential for disease modeling, drug screening and toxicity evaluation. High-throughput optical AP recordings utilizing intramolecular Förster resonance energy transfer (FRET) of the voltage-sensitive fluorescent protein (VSFP) have emerged as a substitute or complement to the resource-intensive patch clamp technique. Here, we functionally validated our recently generated voltage indicator hiPSC lines stably expressing CAG-promoter-driven VSFP in the AAVS1 safe harbor locus. By combining subtype-specific cardiomyocyte differentiation protocols, we established optical AP recordings in ventricular, atrial, and nodal CMs in 2D monolayers using fluorescence microscopy. Moreover, we achieved high-throughput optical AP measurements in single hiPSC-derived CMs in a 3D context. Overall, this system greatly expands the spectrum of possibilities for high-throughput, non-invasive and long-term AP analyses in cardiovascular research and drug discovery.
Apoptosis-mediated anti-proliferative activity of Calligonum comosum against human breast cancer cells, and molecular docking of its major polyphenolics to Caspase-3
Authors: Kumar Ashok , Alfhili Mohammad A. , Bari Ahmed , Ennaji Hanane , Ahamed Maqusood , Bourhia Mohammed , Chebaibi Mohamed , Benbacer Laila , Ghneim Hazem K. , Abudawood Manal , Alghamdi Khalid M. , Giesy John P. , Al-Sheikh Yazeed A. , Aboul-Soud Mourad A. M.
Published: October 2022

Due to poor diagnosis breast cancer in women has emerged as the most common cause of death disease in developing countries. Medicinal plants have been used for thousands of years and can be useful in healthcare, especially in developing countries. Ethanol extracts of leaves of fire bush or arta (Calligonum comosum; EECC), exhibited significant anticancer potencies against two breast cancer cell lines, MCF-7 and MDA 231. These in vitro effects of EECC indicated potential anticancer activities that were determined to be specific since minimal toxicity was recorded against MCF-12, a non-cancerous breast cell line used as a reference. EECC also induced cell cycle arrest in MCF-7 and MDA 231 as revealed by the increased proportions of sub-G1 cells. Fluorescence-activated cell sorter analysis (FACS), utilizing double staining by annexin V-FITC/propidium iodide, revealed that the observed cytotoxic effects were mediated via apoptosis and necrosis. FACS measurement of thegreater in fluorescence intensity, linked with oxidation of DCFH to DCF, revealed that apoptosis was attributable to production of free radicals. EECC-mediated apoptosis was further validated by observation of up-regulation in the “executioner” enzyme, caspase 3. The current findings reveal that EECC exhibits significant, selective cytotoxicity to breast cancer cells, that proceeds via the generation of ROS, which culminates in apoptosis. The anti-proliferative effects of EECC weres further verified by use of a structure-based, virtual screening between its major bioactive polyphenolic constituents and the apoptosis executioner marker enzyme, caspase-3. Based on their glide score values against the active site of caspase 3, some phyto-constituents present in EECC, such as DL-alpha-tocopherol and campesterol, exhibited distinctive, drug-like potential with no predicted toxicity to non-target cells. Taken together, the usefulness of natural phenolic and flavonoid compounds contained in Calligonum comosum were suggested to be potent anticancer agents.
Utilization of amino acid for selective leaching of critical metals from spent hydrodesulfurization catalyst
Authors: Phann Idol , Tanaka Yu , Yamamoto Sae , Okibe Naoko
Published: October 2022

While spent catalysts can cause serious environmental pollution, they can be considered an essential secondary metal source due to their high critical metal grades. The formation of the amino acid-metal complex is often seen in nature, and its potential application in hydrometallurgy can be foreseen. Alanine (Ala) was first screened as the most effective type of amino acid to be used for the selective leaching of spent hydrodesulfurization catalyst (consisting of MoS2 and Co3S4 supported on Al2O3, at 10% Mo and 2.4% Co grades). The sequential 3-step leaching (Step-1: Alkaline Ala leaching at 45°C, Step-2: Hot water leaching at 70°C, Step-3: Second alkaline Ala leaching at 45°C) was conducted where the role of Ala was found to be at least three-fold; 1) maintaining alkalinity by amino acid’s buffering capacity to assist Mo leaching, 2) selectively precipitating Co by forming Co-Ala complex with a distinctive pink color, which can readily re-dissolve in hot water to be separated from spent catalyst particles. 3) Effectively suppressing unwanted dissolution of Al throughout the reaction without needing pH control. Consequently, highly metal-selective, two separate Co-rich (<1% Mo and 79% Co dissolved, Al not detected) and Mo-rich (96% Mo, 19% Co, and 2.1% Al dissolved) leachates were obtained. This study highlighted the potential utility of amino acids as non-toxic, alternative metal lixiviant as well as a metal precipitant for selective leaching of critical metals from spent hydrodesulfurization catalyst.
Ti/Ni co-doped perovskite cathode with excellent catalytic activity and CO2 chemisorption ability via nanocatalysts exsolution for solid oxide electrolysis cell
Authors: Zhen Shuying , Zhang Lihong , Xu Chunming , Zhang Ding , Yi Qun , Sun Wang , Sun Kening
Published: October 2022

Carbon dioxide (CO2) gas is the main cause of global warming and has a significant effect on both climate change and human health. In this study, Ni/Ti co-doped Sr1.95Fe1.2Ni0.1Ti0.2Mo0.5O6-δ (SFNTM) double perovskite oxides were prepared and used as solid oxide electrolysis cell (SOEC) cathode materials for effective CO2 reduction. Ti-doping enhances the structural stability of the cathode material and increases the oxygen vacancy concentration. After treatment in 10% H2/Ar at 800°C, Ni nanoparticles were exsolved in situ on the SFNTM surface (Ni@SFNTM), thereby improving its chemisorption and activation capacity for CO2. Modified by the Ti-doping and the in situ exsolved Ni nanoparticles, the single cell with Ni@SFNMT cathode exhibits improved catalytic activity for CO2 reduction, exhibiting a current density of 2.54 A cm−2 at 1.8 V and 800°C. Furthermore, the single cell shows excellent stability after 100 h at 1.4 V, indicating that Ni/Ti co-doping is an effective strategy for designing novel cathode material with high electrochemical performance for SOEC.
Evolution of Mycobacterium tuberculosis drug resistance in the genomic era
Authors: Nimmo Camus , Millard James , Faulkner Valwynne , Monteserin Johana , Pugh Hannah , Johnson Eachan Oliver
Published: October 2022

Mycobacterium tuberculosis has acquired drug resistance to all drugs that have been used against it, including those only recently introduced into clinical practice. Compared to other bacteria, it has a well conserved genome due to its role as an obligate human pathogen that has adapted to a niche over five to ten thousand years. These features facilitate reconstruction and dating of M. tuberculosis phylogenies, giving key insights into how resistance has been acquired and spread globally. Resistance to each new drug has occurred within five to ten years of clinical use and has occurred even more rapidly with recently introduced drugs. In most cases, resistance-conferring mutations come with a fitness cost, but this can be overcome by compensatory mutations which restore fitness to that of wild-type bacteria. It is likely that M. tuberculosis acquires drug resistance while maintaining limited genomic variability due the generation of low frequency within-host variation, combined with ongoing purifying selection causing loss of variants without a clear fitness advantage. However, variants that do confer an advantage, such as drug resistance, can increase in prevalence amongst all bacteria within a host and become the dominant clone. These resistant strains can then be transmitted leading to primary drug resistant infection in a new host. As many countries move towards genomic methods for diagnosis of M. tuberculosis infection and drug resistance, it is important to be aware of the implications for the evolution of resistance. Currently, understanding of resistance-conferring mutations is incomplete, and some targeted genetic diagnostics create their own selective pressures. We discuss an example where a rifampicin resistance-conferring mutation which was not routinely covered by standard testing became dominant. Finally, resistance to new drugs such as bedaquiline and delamanid is caused by individually rare mutations occurring across a large mutational genomic target that have been detected over a short time, and do not provide statistical power for genotype-phenotype correlation – in contrast to longer-established drugs that form the backbone of drug-sensitive antituberculosis therapy. Therefore, we need a different approach to identify resistance-conferring mutations of new drugs before their resistance becomes widespread, abrogating their usefulness.
Bundle formation of sperm: Influence of environmental factors
Authors: Morcillo i Soler Paula , Hidalgo Carlos , Fekete Zoltán , Zalanyi Laszlo , Khalil Islam S. M. , Yeste Marc , Magdanz Veronika
Published: October 2022

Cooperative behaviour of sperm is one of the mechanisms that plays a role in sperm competition. It has been observed in several species that spermatozoa interact with each other to form agglomerates or bundles. In this study, we investigate the effect of physical and biochemical factors that will most likely promote bundle formation in bull sperm. These factors include fluid viscosity, swim-up process, post-thaw incubation time and media additives which promote capacitation. While viscosity does not seem to influence the degree of sperm bundling, swim-up, post-thaw migration time and suppressed capacitation increase the occurrence of sperm bundles. This leads to the conclusion that sperm bundling is a result of hydrodynamic and adhesive interactions between the cells which occurs frequently during prolonged incubation times.
Association between vitamin D and zoledronate-induced acute-phase response fever risk in osteoporotic patients
Authors: Lu Ke , Shi Qin , Gong Ya-qin , Li Chong
Published: October 2022

Objectives

To elucidate the independent correlation between vitamin D content and zoledronate (ZOL)-triggered acute-phase response (APR) fever risk in osteoporotic (OP) patients, and to examine the potential threshold for optimal vitamin D concentrations that prevent the occurrence of ZOL-induced fever.

Methods

This retrospective investigation was based on a prospectively documented database compiled at the Affiliated Kunshan Hospital of Jiangsu University between January 2015 and March 2022. In total, 2095 OP patients, who received ZOL during hospitalization, were selected for analysis. The primary endpoint was the presence (>37.3°C) or absence (≤37.3°C) of fever, quantified by the maximum body temperature, measured within 3 days of ZOL infusion. The exposure variable was the baseline serum 25-hydroxyvitamin D (25[OH]D) levels.

Results

The OP patients with fever exhibited markedly reduced 25(OH)D content than those without fever. Upon adjusting for age, gender, order of infusion of ZOL, main diagnosis, season of blood collection, year of blood collection, calcitonin usage, and beta-C-terminal telopeptide of type I collagen (β-CTX) levels, a 10 ng/mL rise in serum 25(OH)D content was correlated with a 14% (OR, 0.86; 95% CI, 0.76 to 0.98, P-value = 0.0188) decrease in the odds of ZOL-induced fever. In addition, a non-linear relationship was also observed between 25(OH)D levels and fever risk, and the turning point of the adjusted smoothed curve was 35 ng/mL of serum 25(OH)D content.

Conclusions

Herein, we demonstrated the independent negative relationship between serum 25(OH)D content and ZOL-induced fever risk. According to our analysis, 25(OH)D above 35 ng/mL may be more effective in preventing ZOL-induced APR. If this is confirmed, a “vitamin D supplemental period” is warranted prior to ZOL infusion, particularly the first ZOL infusion, to ensure appropriate 25(OH)D levels that protect against ZOL-induced fever.