bluuebunny/crossref_metadata_2025 huggingface dataset:
The Build-Up Construction for Codes over a Commutative Non-Unitary Ring of Order 9
Authors: Alahmadi Adel , Alihia Tamador , Alma Betty Rowena , Galvez Lucky , Solé Patrick
Published: March 2024

The build-up method is a powerful class of propagation rules that generate self-dual codes over finite fields and unitary rings. Recently, it was extended to non-unitary rings of order 4, to generate quasi self-dual codes. In the present paper, we introduce three such propagation rules to generate self-orthogonal, self-dual and quasi self-dual codes over a special non-unitary ring of order 9. As an application, we classify the three categories of codes completely in length at most 3, and partially in lengths 4 and 5, up to monomial equivalence.
The Mass Formula for Self-Orthogonal and Self-Dual Codes over a Non-Unitary Non-Commutative Ring
Authors: Alahmadi Adel , Alshuhail Altaf , Betty Rowena Alma , Galvez Lucky , Solé Patrick
Published: March 2024

In this paper, we derive a mass formula for the self-orthogonal codes and self-dual codes over a non-commutative non-unitary ring, namely, Ep=a,b|pa=pb=0,a2=a,b2=b,ab=a,ba=b, where a≠b and p is any odd prime. We also give a classification of self-orthogonal codes and self-dual codes over Ep, where p=3,5, and 7, in short lengths.
Switching Self-Attention Text Classification Model with Innovative Reverse Positional Encoding for Right-to-Left Languages: A Focus on Arabic Dialects
Authors: Baniata Laith H. , Kang Sangwoo
Published: March 2024

Transformer models have emerged as frontrunners in the field of natural language processing, primarily due to their adept use of self-attention mechanisms to grasp the semantic linkages between words in sequences. Despite their strengths, these models often face challenges in single-task learning scenarios, particularly when it comes to delivering top-notch performance and crafting strong latent feature representations. This challenge is more pronounced in the context of smaller datasets and is particularly acute for under-resourced languages such as Arabic. In light of these challenges, this study introduces a novel methodology for text classification of Arabic texts. This method harnesses the newly developed Reverse Positional Encoding (RPE) technique. It adopts an inductive-transfer learning (ITL) framework combined with a switching self-attention shared encoder, thereby increasing the model’s adaptability and improving its sentence representation accuracy. The integration of Mixture of Experts (MoE) and RPE techniques empowers the model to process longer sequences more effectively. This enhancement is notably beneficial for Arabic text classification, adeptly supporting both the intricate five-point and the simpler ternary classification tasks. The empirical evidence points to its outstanding performance, achieving accuracy rates of 87.20% for the HARD dataset, 72.17% for the BRAD dataset, and 86.89% for the LABR dataset, as evidenced by the assessments conducted on these datasets.
Multi-Criteria Decision under Uncertainty as Applied to Resource Allocation and Its Computing Implementation
Authors: Ekel Petr Iakovlevitch , Libório Matheus Pereira , Ribeiro Laura Cozzi , Ferreira Mateus Alberto Dorna de Oliveira , Pereira Junior Joel Gomes
Published: March 2024

This research addresses the problem of multi-objective resource allocation or resource deficits, offering robust answers to planning decisions that involve the elementary question: “How is it done?”. The solution to the problem is realized using the general scheme of multi-criteria decision-making in uncertain conditions. The bases of the proposed scheme are associated with the possibilistic approach, which involves the generalization of fuzzy sets from the classical approach to process the uncertainty of information to produce robust (non-dominated) solutions in multi-criteria analysis. Applying this general scheme makes it possible to reduce regions of decision uncertainty through the maximum use of available quantitative information. In the case where quantitative information analysis is insufficient to obtain a unique solution, the proposed approach presupposes the appropriation of qualitative data extracted from experts, who express their opinions considering their knowledge, experience, and intuition. The information on the qualitative character can be represented in diverse preference formats processed by transformation functions to provide homogeneous information for decision procedures used at the final decision stage. The presented results have been implemented within the system of multi-criteria decision-making under uncertain conditions described in the paper. Its functioning is illustrated by solving the typical problem in investment planning activities.
Advances in Metal Casting Technology: A Review of State of the Art, Challenges and Trends—Part II: Technologies New and Revived
Authors: Lehmhus Dirk
Published: March 2024

The present text is the second part of an editorial written for a Special Issue entitled Advances in Metal Casting Technology [...]
Effect of Carbides on Thermos-Plastic and Crack Initiation and Expansion of High-Carbon Chromium-Bearing Steel Castings
Authors: Feng Qian , Zeng Yanan , Li Junguo , Wang Yajun , Tang Guozhang , Wang Yitong
Published: March 2024

The bearing steel’s high-temperature brittle zone (1250 °C–1100 °C), second brittle zone (1100 °C–950 °C), and low-temperature brittle zone (800 °C–600 °C) were determined by the reduction in area and true fracture toughness. The crack sensitivity was strongest at temperatures of 1200 °C, 1000 °C, and 600 °C, respectively. Various experimental and computational methods were used to establish the phase type, microstructure, size, and mechanical properties of carbides in bearing steel. The critical conditions for crack initiation in the matrix (FCC-Fe, FCC-Fe, and BCC-Fe)/carbides (striped Fe0.875Cr0.125C, netted Fe2.36Cr0.64C, and spherical Fe5.25Cr1.75C3) were also investigated. The values for the high-temperature brittle zone, the second brittle zone, and the low-temperature brittle zone were 13.85 MPa and 8.21 × 10−3, 4.64 MPa and 6.52 × 10−3, and 17.86 MPa and 1.86 × 10−2, respectively. These were calculated using Eshelby’s theory and ABAQUS 2021 version software. The ability of the three carbides to cause crack propagation was measured quantitatively by energy diffusion: M3C > MC > M7C3. This study analyzed the mechanism of carbide precipitation on the formation of high-temperature cracks in bearing steel casting. It also provided the critical conditions for carbide/matrix interface cracks in bearing steel continuous casting, thus providing effective support for improving the quality of bearing steel casting.
Fracture Behaviour of Aluminium Alloys under Coastal Environmental Conditions: A Review
Authors: Alqahtani Ibrahim , Starr Andrew , Khan Muhammad
Published: March 2024

Aluminium alloys have been integral to numerous engineering applications due to their favourable strength, weight, and corrosion resistance combination. However, the performance of these alloys in coastal environments is a critical concern, as the interplay between fracture toughness and fatigue crack growth rate under such conditions remains relatively unexplored. This comprehensive review addresses this research gap by analysing the intricate relationship between fatigue crack propagation, fracture toughness, and challenging coastal environmental conditions. In view of the increasing utilisation of aluminium alloys in coastal infrastructure and maritime industries, understanding their behaviour under the joint influences of cyclic loading and corrosive coastal atmospheres is imperative. The primary objective of this review is to synthesise the existing knowledge on the subject, identify research gaps, and propose directions for future investigations. The methodology involves an in-depth examination of peer-reviewed literature and experimental studies. The mechanisms driving fatigue crack initiation and propagation in aluminium alloys exposed to saltwater, humidity, and temperature variations are elucidated. Additionally, this review critically evaluates the impact of coastal conditions on fracture toughness, shedding light on the vulnerability of aluminium alloys to sudden fractures in such environments. The variability of fatigue crack growth rates and fracture toughness values across different aluminium alloy compositions and environmental exposures was discussed. Corrosion–fatigue interactions emerge as a key contributor to accelerated crack propagation, underscoring the need for comprehensive mitigation strategies. This review paper highlights the pressing need to understand the behaviour of aluminium alloys under coastal conditions comprehensively. By revealing the existing research gaps and presenting an integrated overview of the intricate mechanisms at play, this study aims to guide further research and engineering efforts towards enhancing the durability and safety of aluminium alloy components in coastal environments.
The Effect of Dietary Protein Hydrolysate from Black Soldier Fly Larvae and Schizochytrium on Palatability, Nutrient Metabolites and Health Status in Beagle Dogs
Authors: Wei Yu , Xue Lingfeng , Ma Deying , Weng Yuxiao , Liu Mingkang , Li Luyang , Dai Ziyi , Zhao Ziyun , Wang Haifeng , Xu Xiao
Published: March 2024

Protein hydrolysate from black soldier fly larvae (BSFP) has garnered great attention with its lower allergenicity, high amount of essential amino acids, and small bioactive peptides. Schizochytrium is a promising alternative source of n-3 FUFA because it has enriched docosahexaenoic acid (DHA, C22: 6). The aim of this study was to assess palatability, the presence of diarrhea, plasma biochemistry panels, anti-oxidative and anti-inflammatory effects, and immune function in beagle dogs when supplementing a mixture of protein hydrolysate from black soldier fly larvae and schizochytrium (BSFPs) into their diets. Experiment I: 24 young beagle dogs (16 males and 8 females; 4–5 months; BW: 6.40 ± 0.15 kg) were randomly divided into four groups: (1) control (CON), (2) 5% BSFPs, (3) 10% BSFPs, (4) 15% BSFPs. Their body weights and fecal scores were recorded, and blood samples were collected for analysis. Experiment II: three diets containing 5%, 10%, and 15% BSFPs were evaluated by comparing them with a basal diet (CON) to evaluate palatability. These results suggested that a lower presence of diarrhea existed in the BSFP diet than the CON diet (p < 0.05). Three treatment groups remarkably increased their total protein (TP) and albumin (ALB) contents and decreased their concentrations of triglyceride (TG) and total cholesterol (TC) in plasma (p < 0.05). Moreover, the 5% and 15% BSFPs groups had a higher calcium (CA) content in plasma, and the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and contents of creatinine (CREA) and urea nitrogen (BUN) were significantly reduced by supplementing BSFP in their diets (p < 0.05). Their anti-oxidative enzyme activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) were dramatically enhanced, and their malondialdehyde (MDA) concentrations were remarkably reduced (p < 0.05). Immunoglobulin A and G (IgA and IgG) concentrations in the plasma in the 10% and 15% BSFPs groups were significantly increased (p < 0.05). Furthermore, lower interleukin-8 (IL-8) contents were shown in the BSFP diets than the CON diet (p < 0.05). Similarly, the diets supplemented with BSFPs exhibited a positive effect on palatability (p < 0.05). To sum up, the diets supplemented with BSFPs significantly enhanced palatability, immune function, and anti-oxidative and anti-inflammatory capacity to alleviate diarrhea and improve the general health of the beagle dogs.
Enhancing Manufacturability of SU-8 Piezoelectric Composite Films for Microsystem Applications
Authors: Vazquez Irma Rocio , Guler Zeynel , Jackson Nathan
Published: March 2024

Piezoelectric thin films are extensively used as sensing or actuating layers in various micro-electromechanical systems (MEMS) applications. However, most piezoelectrics are stiff ceramics, and current polymer piezoelectrics are not compatible with microfabrication due to their low Curie Temperature. Recent polymer-composite piezoelectrics have gained interest but can be difficult to pattern. Photodefinable piezoelectric films could resolve these challenges by reducing the manufacturability steps by eliminating the etching process. But they typically have poor resolution and thickness properties. This study explores methods of enhancing the manufacturability of piezoelectric composite films by optimizing the process parameters and synthesis of SU-8 piezo-composite materials. Piezoelectric ceramic powders (barium titanate (BTO) and lead zirconate titanate (PZT)) were integrated into SU-8, a negative epoxy-based photoresist, to produce high-resolution composites in a non-cleanroom environment. I-line (365 nm) light was used to enhance resolution compared to broadband lithography. Two variations of SU-8 were prepared by thinning down SU-8 3050 and SU-8 3005. Different weight percentages of the piezoelectric powders were investigated: 5, 10, 15 and 20 wt.% along with varied photolithography processing parameters. The composites’ transmittance properties were characterized using UV-Vis spectroscopy and the films’ crystallinity was determined using X-ray diffraction (XRD). The 0–3 SU-8/piezo composites demonstrated resolutions < 2 μm while maintaining bulk piezoelectric coefficients d33 > 5 pm V−1. The films were developed with thicknesses >10 μm. Stacked layers were achieved and demonstrated significantly higher d33 properties.
Experimental Investigation of Reflectarray Antennas for High-Power Microwave Applications
Authors: Zhao Jianing , Dong Yongzhen , Li Hao , Li Tianming , Liu Wei , Zhou Yihong , Wang Haiyang , Hu Biao , Li Fang , Wang Keqiang , Qiu Bin
Published: March 2024

The power capacity of reflectarray antennas (RAs) is investigated through full-wave simulations and high-power microwave (HPM) experiments in this paper. In order to illustrate the results in detail, two RA elements are designed. The simulated power handling capacity of two RA elements are 7.17 MW/m2 and 2.3 GW/m2, respectively. To further study the HPM RA, two RA prototypes operating at 2.8 GHz are constructed with the aperture size of 1 m × 1 m. Simulations and experimental measurements are conducted for the two prototypes. The experimental results demonstrate that, even when subjected to 1 GW of power, the radiation beam of the RA with the second elements can still propagate in the intended direction. This research will establish a basis for advancing the practicality of RAs in HPM applications.
The Renin–Angiotensin System (RAS) in COVID-19 Disease: Where We Are 3 Years after the Beginning of the Pandemic
Authors: Prato Marco , Tiberti Natalia , Mazzi Cristina , Gobbi Federico , Piubelli Chiara , Longoni Silvia Stefania
Published: March 2024

The RAS is a hormonal system playing a pivotal role in the control of blood pressure and electrolyte homeostasis, the alteration of which is associated with different pathologies, including acute respiratory distress syndrome (ARDS). As such, it is not surprising that a number of studies have attempted to elucidate the role and balance of the renin–angiotensin system (RAS) in COVID-19. In this review article, we will describe the evidence collected regarding the two main enzymes of the RAS (i.e., ACE and ACE2) and their principal molecular products (i.e., AngII and Ang1-7) in SARS-CoV-2 infection, with the overarching goal of drawing conclusions on their possible role as clinical markers in association with disease severity, progression, and outcome. Moreover, we will bring into the picture new experimental data regarding the systemic activity of ACE and ACE2 as well as the concentration of AngII and Ang1-7 in a cohort of 47 COVID-19 patients hospitalized at the IRCCS Sacro Cuore-Don Calabria Hospital (Negrar, Italy) between March and April 2020. Finally, we will discuss the possibility of considering this systemic pathway as a clinical marker for COVID-19.
Eddavidite, Cu12Pb2O15Br2, a New Mineral Species, and Its Solid Solution with Murdochite, Cu12Pb2O15Cl2
Authors: Rosenblatt Melli , Origlieri Marcus J. , Graeme Richard , Graeme Richard , Graeme Douglas , Downs Robert T.
Published: March 2024

Eddavidite is a new mineral species (IMA2018-010) with ideal formula, Cu12Pb2O15Br2, and cubic Fm3¯m symmetry: a = 9.2407(9) Å; V = 789.1(2) Å3; Z = 2. Eddavidite is the bromine analog of murdochite, Cu12Pb2O15Cl2, with which it forms a solid solution series. The type locality is the Southwest mine, Bisbee, Cochise County, Arizona, U.S.A. Eddavidite also occurs in the Ojuela mine, Mapimí, Durango, Mexico. Eddavidite occurs as domains within mixed murdochite–eddavidite crystals. The empirical formula, normalized to 12 Cu apfu, is Cu12(Pb1.92Fe0.06Si0.06)(O15.08F0.02)-(Br0.99Cl0.89☐0.12). Type locality samples contain up to 67% eddavidite component, while Ojuela mine samples contain up to 62%. Mixed eddavidite–murdochite crystals show forms {100} and {111}; the habit grades from cubic through cuboctahedral to octahedral. Mixed eddavidite–-murdochite crystals exhibit good cleavage on {111}. Eddavidite is black, opaque with submetallic luster, and visually indistinguishable from intergrown murdochite. Its Mohs hardness is 4; dmeas. = 6.33 g/cm3, dcalc. = 6.45 g/cm3. The crystal structure, refined to R = 0.0112, consists of corner-sharing square planar CuO4 units, arranged in Cu12O24 metal oxide clusters, which encapsulate Br atoms. PbO8 cubes share edges with Cu12O24 clusters in a continuous framework. Eddavidite incorporates bromine remaining after desiccation of paleo-seawater at its two known localities, which were both once situated along the Western Interior Seaway.
Immobilization Forms of Cadmium and Mercury in a Potassium-Activated Metakaolin-Based Geopolymer
Authors: Prihutami Pramesti , Chaerun Raudhatul , Ohya Yusuke , Otake Tsubasa , Kikuchi Ryosuke , Sato Tsutomu
Published: March 2024

Previous studies of cadmium and mercury immobilization in geopolymers have produced inconsistent results due to their different pozzolans, metal concentrations, and mixing procedures. Understanding the effects of these parameters on heavy metal immobilization is key to predicting their long-term stability. In this study, cadmium and mercury were incorporated into a metakaolin-based K-activated geopolymer by three mixing procedures and concentrations of 0.02–1.00 wt.%. The samples were then immersed in water for 90 d to determine their stability. The results show that mercury is readily leached from the geopolymer, but cadmium is retained. Adding the heavy metals in salt form converts the metals into cadmium hydroxide and mercury oxide that reside at the bottom of the geopolymer. Mixing the salts with water forms soluble heavy metals prior to geopolymerization. This procedure produces more-homogeneous geopolymers. Cadmium is associated with silicate and aluminate, giving a better stability, whereas mercury forms mercury oxide. Different cadmium and mercury concentrations do not change the metal speciation as mercury is affected by relativistic contribution.
Improved Vth Stability and Gate Reliability of GaN-Based MIS-HEMTs by Employing Alternating O2 Plasma Treatment
Authors: Xie Xinling , Wang Qiang , Pan Maolin , Zhang Penghao , Wang Luyu , Yang Yannan , Huang Hai , Hu Xin , Xu Min
Published: March 2024

The Vth stability and gate reliability of AlGaN/GaN metal–insulator–semiconductor high-electron-mobility transistors (MIS-HEMTs) with alternating O2 plasma treatment were systematically investigated in this article. It was found that the conduction band offset at the Al2O3/AlGaN interface was elevated to 2.4 eV, which contributed to the suppressed gate leakage current. The time-dependent dielectric breakdown (TDDB) test results showed that the ALD-Al2O3 with the alternating O2 plasma treatment had better quality and reliability. The AlGaN/GaN MIS-HEMT with the alternating O2 plasma treatment demonstrated remarkable advantages in higher Vth stability under high-temperature and long-term gate bias stress.
Effect of n-C-S-H on Hydration and Reinforcement of Mineral Powder-Cement System at Low Temperatures
Authors: Li Wei , Qian Chunxiang , Li Qingchao , Wang Kehan , Zheng Chunyang , Zhang Yanli
Published: March 2024

This paper investigated the effect of nano-calcium silicate hydrate (n-C-S-H) on the early compressive strength of mineral powder-cement systems under low-temperature curing conditions (5 °C). The hydration mechanism of n-C-S-H in the mineral powder-cement system at different dosages was analyzed by combining it with XRD, DSC-TG, MIP, and other techniques. The results show that n-C-S-H significantly enhances the early compressive strength of the mineral powder-cement system under low-temperature curing conditions, with optimal results observed at a dosage of 1.0% (mass fraction). The XRD, DSC-TG, and MIP tests reveal that n-C-S-H promotes the hydration of the mineral powder cement, accelerates the generation rate of hydration products, reduces the porosity of the hardened mineral powder-cement slurry, and improves the system’s density.
Potassium Intake and Human Health
Authors: D’Elia Lanfranco
Published: March 2024

Potassium is a monovalent cation widely present in nature, where it is not in metallic form, but always in combination with other substances, especially chloride [...]
Oleuropein Supplementation Ameliorates Long-Course Diabetic Nephropathy and Diabetic Cardiomyopathy Induced by Advanced Stage of Type 2 Diabetes in db/db Mice
Authors: Zheng Shujuan , Geng Ruixuan , Guo Jingya , Kang Seong-Gook , Huang Kunlun , Tong Tao
Published: March 2024

Previous studies have reported the therapeutic effects of oleuropein (OP) consumption on the early stage of diabetic nephropathy and diabetic cardiomyopathy. However, the efficacy of OP on the long-course of these diabetes complications has not been investigated. Therefore, in this study, to investigate the relieving effects of OP intake on these diseases, and to explore the underlying mechanisms, db/db mice (17-week-old) were orally administrated with OP (200 mg/kg bodyweight) for 15 weeks. We found that OP reduced expansion of the glomerular mesangial matrix, renal inflammation, renal fibrosis, and renal apoptosis. Meanwhile, OP treatment exerted cardiac anti-fibrotic, anti-inflammatory, and anti-apoptosis effects. Notably, transcriptomic and bioinformatic analyses indicated 290 and 267 differentially expressed genes in the kidney and heart replying to OP treatment, respectively. For long-course diabetic nephropathy, OP supplementation significantly upregulated the cyclic guanosine monophosphate-dependent protein kinase (cGMP–PKG) signaling pathway. For long-course diabetic cardiomyopathy, p53 and cellular senescence signaling pathways were significantly downregulated in response to OP supplementation. Furthermore, OP treatment could significantly upregulate the transcriptional expression of the ATPase Na+/K+ transporting subunit alpha 3, which was enriched in the cGMP–PKG signaling pathway. In contrast, OP treatment could significantly downregulate the transcriptional expressions of cyclin-dependent kinase 1, G two S phase expressed protein 1, and cyclin B2, which were enriched in p53 and cellular senescence signal pathways; these genes were confirmed by qPCR validation. Overall, our findings demonstrate that OP ameliorated long-course diabetic nephropathy and cardiomyopathy in db/db mice and highlight the potential benefits of OP as a functional dietary supplement in diabetes complications treatment.
Glutathione Supplementation Prevents Neonatal Parenteral Nutrition-Induced Short- and Long-Term Epigenetic and Transcriptional Disruptions of Hepatic H2O2 Metabolism in Guinea Pigs
Authors: Mungala Lengo Angela , Mohamed Ibrahim , Lavoie Jean-Claude
Published: March 2024

The parenteral nutrition (PN) received by premature newborns is contaminated with peroxides that induce global DNA hypermethylation via oxidative stress. Exposure to peroxides could be an important factor in the induction of chronic diseases such as those observed in adults who were born preterm. As endogenous H2O2 is a major regulator of glucose–lipid metabolism, our hypothesis was that early exposure to PN induces permanent epigenetic changes in H2O2 metabolism. Three-day-old guinea pigs were fed orally (ON), PN or glutathione-enriched PN (PN+GSSG). GSSG promotes endogenous peroxide detoxification. After 4 days, half the animals were sacrificed, and the other half were fed ON until 16 weeks of age. The liver was harvested. DNA methylation and mRNA levels were determined for the SOD2, GPx1, GCLC, GSase, Nrf2 and Keap1 genes. PN induced GPx1 hypermethylation and decreased GPx1, GCLC and GSase mRNA. These findings were not observed in PN+GSSG. PN+GSSG induced Nrf2 hypomethylation and increased Nrf2 and SOD2 mRNA. These observations were independent of age. In conclusion, in neonatal guinea pigs, PN induces epigenetic changes, affecting the expression of H2O2 metabolism genes. These changes persist for at least 15 weeks after PN. This disruption may signify a permanent reduction in the capacity to detoxify peroxides.
The Influence of L-Lysine-Alpha-Oxidase on the Biofilm Formation of Opportunistic Microorganisms Associated with Inflammatory Diseases of the Urinary Tract
Authors: Senyagin Alexandr , Sachivkina Nadezhda , Das Milana , Arsenyuk Anna , Mannapova Ramziya , Mannapov Alfir , Kubatbekov Tursumbai , Svistunov Dmitriy , Petrukhina Olesya , Zharov Andrey , Zhabo Natallia
Published: March 2024

Urinary tract infections occupy a special niche among diseases of infectious etiology. Many microorganisms associated with urinary tract infections, such as Klebsiella oxytoca, Enterococcus spp., Morganella morganii, Moraxella catarrhalis, Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus, Staphylococcus spp., and Candida spp., can form biofilms. The aim of this research was to study the effect of the enzyme L-lysine-Alpha-oxidase (LO) produced by the fungus Trichoderma harzianum Rifai on the biofilm formation process of microorganisms associated with urinary tract infections. Homogeneous LO showed a more pronounced effect than the culture liquid concentrate (cCL). When adding samples at the beginning of incubation, the maximum inhibition was observed in relation to Enterococcus faecalis 5960—cCL 86%, LO 95%; Enterococcus avium 1669—cCL 85%, LO 94%; Enterococcus cloacae 6392—cCL 83%, LO—98%; and Pseudomonas aeruginosa 3057—cCL 70%, LO—82%. The minimum inhibition was found in Candida spp. Scanning electron microscopy was carried out, and numerous morphological and structural changes were observed in the cells after culturing the bacterial cultures in a medium supplemented with homogeneous LO. For example, abnormal division was detected, manifesting as the appearance of joints in places where the bacteria diverge. Based on the results of this work, we can draw conclusions about the possibility of inhibiting microbial biofilm formation with the use of LO; especially significant inhibition was achieved when the enzyme was added at the beginning of incubation. Thus, LO can be a promising drug candidate for the treatment or prevention of infections associated with biofilm formation.
Anti-Inflammatory Effect of Columbianadin against D-Galactose-Induced Liver Injury In Vivo via the JAK2/STAT3 and JAK2/p38/NF-κB Pathways
Authors: Ma Zhe , Peng Lin , Sheng Yaoyao , Chu Wenhui , Fu Yongqian
Published: March 2024

Angelicae pubescentis radix (APR) has been traditionally used for thousands of years in China to treat rheumatoid arthritis (RA), an autoimmune disorder. As the main active coumarin of APR, columbianadin (CBN) exhibits a significant anti-inflammatory effect in vitro. However, the anti-inflammatory activity and underlying mechanism of CBN in vivo remain unclear. This work aimed to elucidate the anti-inflammatory activity of CBN in vivo and its related signaling pathways in a D-Gal-induced liver injury mouse model. Analysis of biochemical indices (ALT and AST) and pro-inflammatory cytokines (IL-1β and IL-6) in serum indicated that CBN significantly ameliorated D-Gal-induced liver injury. CBN treatment also significantly increased the activities of antioxidant enzymes (SOD, CAT, GPx), and decreased the levels of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) in liver tissue. Liver histology revealed that CBN treatment reduced hepatic inflammation. Western blot analysis indicated that CBN down-regulates the expression of phosphorylated JAK2, STAT3, MAPK, and NF-κB in the related signaling pathways. These findings support the traditional use of APR as a remedy for the immune system, and indicate that the JAK2/STAT3 and JAK2/p38/NF-κB signaling pathways may be important mechanisms for the anti-inflammatory activity of CBN in vivo.
Efficient Structure Transformation Based on Sensitivity-Oriented Structure Adjustment for Inverse-Designed Devices
Authors: Chen Yuchen , Qiu Jifang , Dong Zhenli , Wang Lihang , Wu Lan , Jiao Suping , Guo Hongxiang , Wu Jian
Published: March 2024

Inverse-designed devices with thousands of degrees of freedom could achieve high performance in compact footprints, but typically have complex structure topologies that contain many irregular and tiny features and sharp corners, which tend to lead to a poor robustness to fabrication errors. In order to effectively transform the structure of inverse-designed nanophotonic devices into simple structure topologies that have high robustness to fabrication errors without sacrificing device performance, in this paper, we propose a structure adjustment method that innovatively adjusts the structures of inverse-designed devices by introducing their structural sensitivity to the optical performance, extracting the device substructures with high sensitivity and eliminating those with low sensitivity, and, finally, transforming the device structures into simple structure topologies with high robustness and better performance. Two devices (90° crossing and T-junction) were designed and fabrication tolerance simulation was conducted to verify the method. The results show that the devices designed using the proposed method achieved better performance and were more robust to under/over-etched errors.
Modal Phase Modulators Based on Liquid Crystals with 3D-Printed Polymer Microstructures: Increasing Size and Complexity
Authors: Xu Alec , Nourshargh Camron , Salter Patrick S. , Elston Steve J. , Morris Stephen M. , Booth Martin J.
Published: March 2024

We present extended capabilities in simple liquid crystal-based devices that are applicable to adaptive optics and other related fields requiring wavefront manipulation. The laser-written devices can provide complex phase profiles, but are extremely simple to operate, requiring only a single electrode pair tuned between 0 and 10 V RMS. Furthermore, the devices operate in the transmissive mode for easy integration into the optical path. We present here as examples three such devices: the first device reproduces the defocus Zernike polynomial; the second device reproduces a seventh-order Zernike polynomial, tertiary coma; and the last example is of a primary spherical aberration. All devices offer wavelength-scale wavefront manipulation up to more than 2π radians peak-to-peak phase at a wavelength of 660 nm. The coma correction device is significantly more complex, reproducing a mode two orders higher than previous demonstrations, while the spherical device is nearly a full order of magnitude larger, measuring 2 mm in diameter.
Optimization of Grating Coupler over Single-Mode Silicon-on-Insulator Waveguide to Reach < 1 dB Loss through Deep-Learning-Based Inverse Design
Authors: Lin Chung-Chih , Na Audrey , Wu Yi-Kuei , Wang Likarn , Na Neil
Published: March 2024

Grating couplers are essential components in silicon photonics that facilitate the coupling of light between waveguides and fibers. Optimization of the grating couplers to reach <1 dB loss when coupling to single-mode fibers (SMFs) has been reported in the literature, but this was based on silicon-on-insulator (SOI) waveguides supporting multi-modes. In this paper, using a deep-learning model combined with an inverse-design process, we achieve <1 dB losses for grating couplers implemented over single-mode SOI waveguides, i.e., a maximum efficiency of 80.5% (−0.94 dB) for gratings constrained with e-beam (EB) lithography critical dimension (CD), and a maximum efficiency of 77.9% (−1.09 dB) for gratings constrained with deep ultraviolet (DUV) lithography CD. To verify these results, we apply covariance matrix adaptation evolution strategy (CMA-ES) and find that while CMA-ES yields slightly better results, i.e., 82.7% (−0.83 dB) and 78.9% (−1.03 dB) considering e-beam and DUV, respectively, the spatial structures generated by CMA-ES are nearly identical to the spatial structures generated by the deep-learning model combined with the inverse-design process. This suggests that our approach can achieve a representative low-loss structure, and may be used to improve the performance of other types of nanophotonic devices in the future.
Antioxidative Response and Phenolic Content of Young Industrial Hemp Leaves at Different Light and Mycorrhiza
Authors: Varga Ivana , Kristić Marija , Lisjak Miroslav , Tkalec Kojić Monika , Iljkić Dario , Jović Jurica , Kristek Suzana , Markulj Kulundžić Antonela , Antunović Manda
Published: March 2024

Due to the increasing presence of industrial hemp (Cannabis sativa L.) and its multiple possibilities of use, the influence of different light and several biopreparations based on beneficial fungi and bacteria on hemp’s morphological and physiological properties were examined. Different biopreparations and their combinations were inoculated on hemp seed and/or substrate and grown under blue and white light. A completely randomized block design was conducted in four replications within 30 days. For biopreparation treatment, vesicular arbuscular mycorrhiza (VAM) in combination with Azotobacter chroococum and Trichoderma spp. were inoculated only on seed or both on seed and in the substrate. Generally, the highest morphological parameters (stem, root and plant length) were recorded on plants in white light and on treatment with applied Trichoderma spp., both on seed and substrate. Blue light negatively affected biopreparation treatments, resulting in lower values of all morphological parameters compared to control. Leaves pigments were higher under blue light, as compared to the white light. At the same time, 1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), flavonoids, total flavanol content and phenolic acids were not influenced by light type. Biopreparation treatments did not significantly influence the leaves’ pigments content (Chl a, Chl b and Car), nor the phenolic and flavanol content.
Potential of Plant-Based Extracts to Alleviate Sorbitol-Induced Osmotic Stress in Cabbage Seedlings
Authors: Pacyga Katarzyna , Pacyga Paweł , Boba Aleksandra , Kozak Bartosz , Wolko Łukasz , Kochneva Yelyzaveta , Michalak Izabela
Published: March 2024

In light of expected climate change, it is important to seek nature-based solutions that can contribute to the protection of our planet as well as to help overcome the emerging adverse changes. In an agricultural context, increasing plant resistance to abiotic stress seems to be crucial. Therefore, the scope of the presented research was focused on the application of botanical extracts that exerted positive effects on model plants growing under controlled laboratory conditions, as well as plants subjected to sorbitol-induced osmotic stress. Foliar spraying increased the length and fresh mass of the shoots (e.g., extracts from Taraxacum officinale, Trifolium pratense, and Pisum sativum) and the roots (e.g., Solidago gigantea, Hypericum perforatum, and Pisum sativum) of cabbage seedlings grown under stressful conditions, as well as their content of photosynthetic pigments (Pisum sativum, Lens culinaris, and Hypericum perforatum) along with total phenolic compounds (Hypericum perforatum, Taraxacum officinale, and Urtica dioica). The antioxidant activity of the shoots measured with the use of DDPH (Pisum sativum, Taraxacum officinale, Urtica dioica, and Hypericum perforatum), ABTS (Trifolium pratense, Symphytum officinale, Valeriana officinalis, Pisum sativum, and Lens culinaris), and FRAP (Symphytum officinale, Valeriana officinalis, Urtica dioica, Hypericum perforatum, and Taraxacum officinale) assays was also enhanced in plants exposed to osmotic stress. Based on these findings, the most promising formulation based on Symphytum officinale was selected and subjected to transcriptomic analysis. The modification of the expression of the following genes was noted: Bol029651 (glutathione S-transferase), Bol027348 (chlorophyll A-B binding protein), Bol015841 (S-adenosylmethionine-dependent methyltransferases), Bol009860 (chlorophyll A-B binding protein), Bol022819 (GDSL lipase/esterase), Bol036512 (heat shock protein 70 family), Bol005916 (DnaJ Chaperone), Bol028754 (pre-mRNA splicing Prp18-interacting factor), Bol009568 (heat shock protein Hsp90 family), Bol039362 (gibberellin regulated protein), Bol007693 (B-box-type zinc finger), Bol034610 (RmlC-like cupin domain superfamily), Bol019811 (myb_SHAQKYF: myb-like DNA-binding domain, SHAQKYF class), Bol028965 (DA1-like Protein). Gene Ontology functional analysis indicated that the application of the extract led to a decrease in the expression of many genes related to the response to stress and photosynthetic systems, which may confirm a reduction in the level of oxidative stress in plants treated with biostimulants. The conducted studies showed that the use of innovative plant-based products exerted positive effects on crops and can be used to supplement current cultivation practices.
Optimizing ChIRP-MS for Comprehensive Profiling of RNA-Protein Interactions in Arabidopsis thaliana: A Telomerase RNA Case Study
Authors: Bozděchová Lucie , Rudolfová Anna , Hanáková Kateřina , Fojtová Miloslava , Fajkus Jiří
Published: March 2024

The current repertoire of methods available for studying RNA-protein interactions in plants is somewhat limited. Employing an RNA-centric approach, particularly with less abundant RNAs, presents various challenges. Many of the existing methods were initially designed for different model systems, with their application in plants receiving limited attention thus far. The Comprehensive Identification of RNA-Binding Proteins by Mass Spectrometry (ChIRP-MS) technique, initially developed for mammalian cells, has been adapted in this study for application in Arabidopsis thaliana. The procedures have been meticulously modified and optimized for telomerase RNA, a notable example of a low-abundance RNA recently identified. Following these optimization steps, ChIRP-MS can serve as an effective screening method for identifying candidate proteins interacting with any target RNA of interest.
Dairy Effluent-Saturated Biochar’s Short-Term Effects on Vigna unguiculata and Cynodon dactylon Performance and Soil Properties
Authors: Entio Lisandro J. , Taggart Cosette B. , Muir James P. , Kan Eunsung , Brady Jeff A. , Obayomi Olabiyi
Published: March 2024

We compared the effects of wood-, manure-, and blend-derived biochar (BC) saturated/unsaturated with dairy effluents on Vigna unguiculata and Cynodon dactylon performance and soil characteristics in a greenhouse pot study. Plant samples were assayed for herbage and root dry weight and N and C percentages. Soil samples were assayed for nutrients, pH, and conductivity. Variance analysis, Tukey’s tests, Pearson’s correlations, and multiple regression analysis were performed. The performance of C. dactylon was not affected. V. unguiculata’s herbage and root production responded negatively to manure BC and 2% of any BC, respectively, which is mainly explained by the conductivity and soil P increase, respectively. When V. unguiculata was grown, BC inclusion decreased NO3-N and increased the soil P content. When C. dactylon was grown, only P was altered (increased) when manure or the blend BC were applied. The soil total C increased as the BC loading rate increased. The application of high BC rates was detrimental for V. unguiculata, but showed a neutral effect for C. dactylon. To improve dairy waste recycling, saturated 1% blend BC and saturated 2% blend or manure BC could be applied to V. unguiculata and C. dactylon, respectively, with no short-term negative impacts. Only wood BC avoided soil P build-up. BC application increased the soil total C, showing potential for C sequestration.
Genome-Wide Association Studies on Chinese Wheat Cultivars Reveal a Novel Fusarium Crown Rot Resistance Quantitative Trait Locus on Chromosome 3BL
Authors: Wang Chuyuan , Sun Manli , Zhang Peipei , Ren Xiaopeng , Zhao Shuqing , Li Mengyu , Ren Zhuang , Yuan Meng , Ma Linfei , Liu Zihan , Wang Kaixuan , Chen Feng , Li Zaifeng , Wang Xiaodong
Published: March 2024

Fusarium crown rot (FCR), primarily caused by Fusarium pseudograminearum, has emerged as a new threat to wheat production and quality in North China. Genetic enhancement of wheat resistance to FCR remains the most effective approach for disease control. In this study, we phenotyped 435 Chinese wheat cultivars through FCR inoculation at the seedling stage in a greenhouse. Our findings revealed that only approximately 10.8% of the wheat germplasms displayed moderate or high resistance to FCR. A genome-wide association study (GWAS) using high-density 660K SNP led to the discovery of a novel quantitative trait locus on the long arm of chromosome 3B, designated as Qfcr.hebau-3BL. A total of 12 significantly associated SNPs were closely clustered within a 1.05 Mb physical interval. SNP-based molecular markers were developed to facilitate the practical application of Qfcr.hebau-3BL. Among the five candidate FCR resistance genes within the Qfcr.hebau-3BL, we focused on TraesCS3B02G307700, which encodes a protein kinase, due to its expression pattern. Functional validation revealed two transcripts, TaSTK1.1 and TaSTK1.2, with opposing roles in plant resistance to fungal disease. These findings provide insights into the genetic basis of FCR resistance in wheat and offer valuable resources for breeding resistant varieties.
The Potential for Foreign Body Reaction of Implanted Poly-L-Lactic Acid: A Systematic Review
Authors: Nonhoff Melanie , Puetzler Jan , Hasselmann Julian , Fobker Manfred , Gosheger Georg , Schulze Martin
Published: March 2024

Poly-L-lactic acid (PLLA) implants have been used for bone fixation for decades. However, upon insertion, they can cause a foreign body reaction (FBR) that may lead to complications. On 15 December 2023, a systematic review was conducted to search for articles on the PubMed, MeSH term, and Scopus databases using the keywords ‘PLLA’ and ‘foreign body reaction’. The articles were reviewed not only for the question of FBR, its severity, and the manifestation of symptoms but also for the type of implant and its location in the body, the species, and the number of individuals included. A total of 71 original articles were identified. Of these, two-thirds reported on in vivo trials, and one-third reported on clinical applications. The overall majority of the reactions were mild in more than half of the investigations. Symptoms of extreme and extensive FBR mainly include osteolysis, ganglion cysts, and swelling. The localization of PLLA implants in bone can often result in osteolysis due to local acidosis. This issue can be mitigated by adding hydroxyapatite. There should be no strong FBR when PLLA is fragmented to 0.5–4 µm by extracorporeal shock wave.
Study of Hygrothermal Aging for Basalt Fiber/Epoxy Resin Composites Modified with CeCl3
Authors: Li Chong , Zhang Longwang , Wang Haoyu , Song Yiguo , Wang Jiayou
Published: March 2024

With increasing attention being paid to environmental issues, the application of natural fibers in fiber-reinforced composites has attracted more and more attention. Composite materials with basalt fibers (BFs) as reinforcement have excellent properties and are widely used in many fields. Hydrothermal aging crucially influences the durability of basalt fiber/epoxy resin composites (BF/ERCs). In this study, BFs were used as reinforcing materials, whose surfaces were modified with a rare earth modification solution (CeCl3). The density, mechanical performance, and chemical properties of BF/ERCs subjected to hygrothermal aging were analyzed by the weight method, static mechanical performance testing, scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FT-IR). The effects of the modification solution with different Ce concentrations on the water absorption, tensile, bending and interlaminar shear strength (ILSS) of BF/ERCs were investigated. The test results showed that the water absorption of BF/ERCs treated with a modification solution that contained Ce 0.5 wt % as the minimum value and the retention rate of the mechanical properties of BF/ERCs reached maximum values after hygrothermal aging.
Enhanced Corrosion Resistance and Mechanical Durability of the Composite PLGA/CaP/Ti Scaffolds for Orthopedic Implants
Authors: Prosolov Konstantin A. , Komarova Ekaterina G. , Kazantseva Ekaterina A. , Luginin Nikita A. , Kashin Alexander D. , Uvarkin Pavel V. , Sharkeev Yurii P.
Published: March 2024

In addressing the challenge of enhancing orthopedic implants, 3D porous calcium phosphate (CaP) coatings on titanium (Ti) substrates modified with poly(lactic-co-glycolic acid) (PLGA) were proposed. CaP coatings on Ti were deposited using the ultrasonic-assisted micro-arc oxidation (UMAO) method, followed by modification with PLGA through a dip coating process at concentrations of 5%, 8%, and 10%. The addition of PLGA significantly improved adhesive–cohesive strength according to the scratch test, while PLGA to CaP adhesion was found to be not less than 8.1 ± 2.2 MPa according to the peel test. Tensile testing showed a typical fracture of CaP coatings and mechanisms of brittle fracture. Corrosion resistance, assessed via gravimetric and electrochemical methods in 0.9% NaCl and PBS solutions, revealed PLGA’s substantial reduction in corrosion rates, with the corrosion current decreasing by two orders of magnitude even for the 5% PLGA/CaP/Ti sample. Also, the PLGA layer significantly enhanced the impedance modulus by two orders of magnitude, indicating a robust barrier against corrosion at all PLGA concentrations. Higher PLGA concentrations offered even greater corrosion resistance and improved mechanical properties. This research underscores the potential of using CaP- and PLGA-modified coatings to extend the life and functionality of orthopedic implants, addressing a significant challenge in biomedical engineering.
Risk Analysis of Wellbore Instability of “Felt Layer” in Qiudong Sag Based on Monte Carlo Method
Authors: Jia Ruixue , Zhu Liang , Song Xiaoping , Chen Jian
Published: March 2024

Both domestic and international scholars have conducted in-depth research on wellbore stability issues. They have established various empirical models, analytical models, and numerical simulation methods. However, there is relatively little research on the impact of the uncertainty of input parameters on wellbore stability, and the understanding of this aspect remains unclear. To address this, this paper introduces a probability distribution method. It is based on a wellbore stability mechanical analytical model and, using reliability theory, establishes a method for evaluating wellbore instability risks. By employing the Monte Carlo random simulation method, this study investigates the sensitivity of input parameters to wellbore stability, clarifying that ground stress is the main controlling factor affecting wellbore stability. Combining the analysis of the “felt layer” ground stress profile, this study utilizes two-dimensional simulation experiments to validate the accurate determination of ground stress magnitude in wellbore stability analysis. It also examines the impact of reducing its uncertainty. The results show that this approach significantly reduces the risk of wellbore instability, addressing the challenging issue of identifying wellbore instability in the Qiu Dong Depression’s “felt layer” within the TH Basin.