bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of laparoscopic and open pancreaticoduodenectomy for the treatment of distal cholangiocarcinoma: A propensity score matching analysis
Authors: Zhu Yuwen , Zu Guangchen , Wu Di , Zhang Yue , Yang Yang , Wu Han , Chen Xuemin , Chen Weibo
Published: November 2022

Background

There are few studies comparing the oncological outcomes of laparoscopic pancreaticoduodenectomy (LPD) and open pancreaticoduodenectomy (OPD) for distal cholangiocarcinoma (DCC). Our objective was to assess the short-term efficacy and long-term survival of LPD and OPD in patients with DCC.

Methods

The data of 124 DCC patients who underwent LPD or OPD at the Third Affiliated Hospital of Soochow University from May 2010 to May 2021 were retrospectively analyzed. Propensity score matching was performed to balance the two groups of baseline characteristics. After 1:1 matching, the overall survival (OS) of the two groups was compared by the Kaplan−Meier method. Univariate and multivariate Cox regression analyses were used to identify independent predictors of OS.

Results

The original cohort consisted of 124 patients. Nineteen patients were excluded because of incomplete baseline or follow-up data, and the remaining 105 patients were divided into two cohorts (45 in the LPD group and 60 in the OPD group). The LPD group showed more favorable results in OS analysis (LPD vs. OPD, 56.4 [46.2-66.5] vs. 48.9 [36.4-61.4], months, P=0. 01). PSM analysis identified 30 pairs of patients, and differences between matching groups were still significant (LPD vs. OPD, 67.9[58.2-77.6] vs. 47.4[31.4-67.5], months, P=0.002). Moreover, the LPD group experienced less intraoperative bleeding (LPD vs. OPD, 292.67 vs. 519.17 mL, P=0.002). Univariate analysis showed that surgical modality (P=0.012), carbohydrate antigen 19-9 (P=0.043), carcinoembryonic antigen (P=0.003), neutrophil-to-lymphocyte ratio (P=0.012), blood transfusion (P=0.031), clinically relevant postoperative pancreatic fistula (P<0.001) and lymphatic metastasis (P=0.004) were predictors of OS. Multivariate Cox analysis demonstrated that carbohydrate antigen 19-9 (P=0.048), carcinoembryonic antigen (P=0.031) and lymphatic metastasis (P=0.023) were independent predictive factors of OS. However, adjuvant therapy had no significant effect on the OS of DCC patients after radical pancreaticoduodenectomy (P>0.05).

Conclusions

For DCC patients, LPD may be a more recommended procedure because of its advantages over OPD in terms of intraoperative bleeding and long-term survival.
Neuro-ophthalmic complications of immune checkpoint inhibitor therapy: Current status and future directions
Authors: Winges Kimberly M. , Gordon Lynn K.
Published: November 2022

Since 2011, use of immune checkpoint inhibitors (ICI) in cancer immunotherapy dramatically expanded, both alone and in combination with either a different cancer treatment or with two different ICIs. With this increase in use have come a myriad of adverse effects from enhanced immune activation, including ophthalmic and neurologic immune related adverse events (irAE). Neuro-ophthalmic immune related adverse events (NOirAE) associated with use of ICIs are increasingly recognized and their severity may actually limit use of potentially life-saving immunotherapy. NOirAEs comprise a wide variety of presentations involving both the central and peripheral nervous system. They cause afferent or efferent visual dysfunction, including among them optic neuropathy and edema, orbital inflammatory disease, and ocular myasthenia. While treatment for irAEs typically involves immunosuppression with corticosteroids, there is no expert consensus regarding best practices for treatment of NOirAEs and whether to stop ICI immunotherapy for the cancer or not. This state-of-the-art review explores the pathophysiologic basis for NOirAEs, provides a framework for categorizing them within neuro-ophthalmology, and discusses what is needed to close the current knowledge gaps in diagnosis and management of an increasing population of cancer patients requiring neuro-ophthalmic care.
A retrospective study on the characteristics of renal pathological grades in HSPN children with mild to moderate proteinuria
Authors: Cao Yan , Shen Tian , Li Yongzhen , Shuai Lanjun , Chen Qiaoping , Mo Shuanghong , Li Canlin , Li Xiaoyan , Wang Ying , Wu Xiaochuan
Published: November 2022

Objective

To investigate the characteristics of renal pathological grades in Henoch–Schönlein purpura nephritis (HSPN) children with mild to moderate proteinuria and the correlation between pathological grade and severity of proteinuria among this population.

Methods

HSPN children who were presented with mild (150 mg <24 h urinary protein <25 mg/kg) to moderate (25 mg/kg ≤24 h urinary protein <50 mg/kg) proteinuria and performed renal biopsy without steroid ± immunosuppressant treatment in the Second Xiangya Hospital between January 2010 and March 2021 were involved. We retrospectively analyzed the correlation between age, disease course, degree of proteinuria, type of immunoglobulin deposits, C3 deposits in glomeruli and renal pathological grade.

Results

(1) 72 HSPN children including 46 boys and 26 girls were included, with a mean age of onset of 9.01 ± 2.65 years old. The majority of these patients (62.5%) had a disease course between 1 week to 1 month. 51 patients presented with mild proteinuria and 21 patients with moderate proteinuria. (2) Renal biopsy results showed that ISKDC Grade IIIa were both predominant in mild proteinuria group (25, 49%) and moderate proteinuria group (11, 52.4%). 32 patients had grade II (44.4%), 2 had grade IIIb (2.8%), 1 had grade IV (1.4%), and 1 had grade VI (1.4%). There was no correlation between age, disease course and renal pathological grade (p > 0.05). (3) In patients with mild proteinuria (n = 51), 27 (52.9%) HSPN children had a pathological grade ≥ grade III. In patients with moderate proteinuria (n = 21), 13 (61.9%) HSPN children had grade ≥ III. There was no significant difference in the proportion of renal pathological grade between the 2 groups (p > 0.05). (4) There was no significant correlation between glomerular C3 deposits or immunoglobulin deposit types and renal pathological grade (p = 0.776 and p = 0.056 respectively).

Conclusion

In HSPN children with mild to moderate proteinuria, longer disease course or heavier urinary protein level is not completely parallel with higher renal pathological grade. ISKDC grade IIIa is the most common pathological grade. Clinicians should pay great attention to the renal injury in patients with mild to moderate proteinuria.
Clinical characteristics and MRI-based phenotypes of perianal abscess formation in children with fistulizing Crohn's Disease
Authors: Tabari Azadeh , Kaplan Jess L. , Huh Susanna Y. , Moran Christopher J. , Gee Michael S.
Published: November 2022

Purpose

The aim of this study was to explore potential correlation of the MR imaging features and clinical characteristics with formation of perianal abscess in children with Crohn's perianal fistulas (CPF).

Methods

From 2010 to 2020, pediatric patients with CPF diagnosis on their first pelvic MRI were identified retrospectively. All patients were divided into two groups based on the presence or absence of perianal abscess. Baseline clinical and MRI characteristics were recorded for each patient. All the statistical calculations were performed using R (version 3.6.3).

Results

A total of 60 patients [F:M 17:43, median age 14 years (IQR 10-15), ranging 3–18 years] were included in this study. Forty-four abscesses were identified in 36/60 children (mean volume 3 ± 8.6 ml, median 0.3 ml). In 24/60 patients with perianal disease, no abscess was detected on the MRI. Ten patients (28%) showed perianal abscess on pelvic MRI at the initial diagnosis. The rate of active disease on colonoscopy (visible ulcerations/aphthous ulcers) was similar in both groups (95% vs. 94%). With regards to disease location, the majority of patients (40/60, 66.6%) in both groups had ileocolonic CD. All patients without abscess had a single perianal fistula (n = 24; 3 simple and 21 complex fistulae), however, patients with perianal abscess tended to have >1 fistulous tracts (n = 50 fistulas; all complex, 27 single, 10 double and 1 triple). Intersphincteric fistula was the most common fistula type in both groups (79% and 66%, p = 0.1). The total length of fistula (3.8 ± 1.7 vs. 2.8 ± 0.8 cm, p = 0.006) and presence of multiple external openings (n = 25 vs. 7, p = 0.019) were significantly higher in patients with abscesses, and fistula length >3.3 cm showed 80% specificity and 83% PPV for the presence of perianal abscess. Fistulas were symptomatic (pain, bleeding or drainage) at similar rates in both groups (68% and 70%, p = 0.1).

Conclusion

Pediatric patients with CPF who develop perianal abscess have a distinct imaging phenotype defined by longer fistula length (>3.3 cm), multiple skin openings and multiple fistulous tracts (≥2) on MRI. Patients who have these features but does not have an abscess on imaging may merit more aggressive treatment (and close monitoring) to prevent the development of an abscess.
Prediction and associated factors of non-steroidal anti-inflammatory drugs efficacy in migraine treatment
Authors: Lu Zhao-Xuan , Dong Bing-Qing , Wei Heng-Le , Chen Liang
Published: November 2022

Background: The selection strategy of non-steroidal anti-inflammatory drugs (NSAIDs) for migraine is hard to judge whether it is effective, leading to unnecessary exposure to insufficient or lengthy treatment trials. The goal of the study was to investigate potential predictors of NSAIDs efficacy in migraine therapy and to explore their influence on efficacy.

Methods: 610 migraine patients were recruited and assigned into responders and non-responders. Potential predictors among demographic and clinical characteristics for NSAIDs efficacy were extracted using multivariable logistic regression (LR) analysis, and were applied to construct prediction models via machine learning (ML) algorithms. Finally, Cochran-Mantel-Haenszel tests were used to examine the impact of each predictor on drug efficacy.

Results: Multivariate LR analysis revealed migraine-related (disease duration, headache intensity and frequency) and psychiatric (anxiety, depression and sleep disorder) characteristics were predictive of NSAIDs efficacy. The accuracies of ML models using support vector machine, decision tree and multilayer perceptron were 0.712, 0.741, and 0.715, respectively. Cochran-Mantel-Haenszel test showed that, for variables with homogeneity of odds ratio, disease duration, frequency, anxiety, and depression and sleep disorder were associated with decreased likelihood of response to all NSAIDs. However, the variabilities in the efficacy of acetaminophen and celecoxib between patients with mild and severe headache intensity were not confirmed.

Conclusion: Migraine-related and psychiatric parameters play a critical role in predicting the outcomes of acute migraine treatment. These models based on predictors could optimize drug selection and improve benefits from the start of treatment.
Bushen huoxue decoction inhibits RANKL-stimulated osteoclastogenesis and glucocorticoid-induced bone loss by modulating the NF-κB, ERK, and JNK signaling pathways
Authors: Liu Yamei , Fu Binlan , Li Xiaoman , Chen Chen , Li Xican , Xu Liangliang , Wang Bin
Published: November 2022

Glucocorticoid-induced osteoporosis (GIOP) is the most common form of secondary osteoporosis, which is caused by a disorder in bone metabolism due to excessive activation of osteoclasts. Bushen Huoxue decoction (BHD) is an herbal formula with multiple pharmacological effects, including anti-inflammatory, antioxidant activity and stem cell migration promotion. However, the effect of BHD on osteoclastogenesis has not been reported. In this study, we aimed to elucidate the effect of BHD on RANKL-stimulated osteoclastogenesis and explored its underlying mechanisms of action in vitro. Our results show that BHD had no effect on BMMs and RAW264.7 cells viability, but inhibited RANKL-induced osteoclast formation in vitro. Furthermore, BHD attenuated RANKL-induced NF-κB, ERK, and JNK signaling. The attenuation of NF-κB, ERK, and JNK activation were enough to impede downstream expression of c-fos and NFATc1 and related specific genes. Meanwhile, we investigated the therapeutic effect of BHD on glucocorticoid-induced osteoporosis (GIOP) mice. The result indicated that BHD prevents glucocorticoid-induced osteoporosis and preserves bone volume by repressing osteoclast activity. Collectively, BHD shows significant osteoclast inhibition and holds great promise in the treatment of osteoporosis.
Metabolomics- and systems toxicology-based hepatotoxicity mechanism of Sophorae Tonkinensis Radix et Rhizoma in rats
Authors: Yu Dengxiang , Shao Zhen , Fu Yuemeng , Tang Xiaohang , Chen Qilong , Deng Zhongping
Published: November 2022

Drug-induced liver injury (DILI) is a major challenge to the development and clinical application of drugs, especially limits the global application of Chinese herbal medicines, because the material basis and mechanisms of some Chinese herbal medicines are not well clear. In this study, a comprehensive method integrating metabolomics and systems toxicology (SysT) was used to investigate how the main substances in Sophorae TonkinensisRadix et Rhizoma (STRER) influence the metabolic pathways and molecular mechanisms of hepatotoxicity. Through a 28-day continuous oral administration toxicity study combined with serum metabolomics analyses, the aqueous, ethanol-precipitation and dichloromethane extracts of STRER exhibited significant hepatotoxic effects. In addition, 19 differential metabolites with a time-dose-effect relationship were identified in rats. The primary bile acid biosynthesis pathway was significantly altered, which was consistent with the findings of the SysT analysis. Furthermore, through the quantification of bile acids in serum, 16 differential bile acids were identified as being significantly changed; moreover, 21 relevant targets which intersected with the hepatotoxic targets of STRER were identified. Molecular docking was used to confirm the validation of bindings between targets and corresponding compounds, and finally, six important compounds and 14 potential targets were identified to be involved in STRER-induced liver injury in relation to bile acid metabolism.
Ocular adverse events associated with anti-VEGF therapy: A pharmacovigilance study of the FDA adverse event reporting system (FAERS)
Authors: Ma Pan , Pan Xinmei , Liu Ruixiang , Qu Ya , Xie Linli , Xie Jiangchuan , Cao Liya , Chen Yongchuan
Published: November 2022

Background: The purpose of this study is to identify and characterize ocular adverse events (AEs) that are significantly associated with anti-VEGF drugs for treatment of neovascular age-related macular degeneration and compare the differences between each drug, and provide clinical reference.

Methods: Ocular AEs submitted to the US Food and Drug Administration were analyzed to map the safety profile of anti-VEGF drugs. The Pharmacovigilance tools used for the quantitative detection of signals were reporting odds ratio and bayesian confidence propagation neural network.

Results: A total of 10,608,503 AE reports were retrieved from FAERS, with 20,836 for ranibizumab, 19,107 for aflibercept, and 2,442 for brolucizumab between the reporting period of Q1, 2004 and Q3, 2021. We found and analyzed the different AEs with the strongest signal in each drug—ranibizumab-macular ischaemia (ROR = 205.27, IC-2SD = 3.70), retinal pigment epithelial tear (ROR = 836.54, IC-2SD = 7.19); aflibercept-intraocular pressure increased (ROR = 31.09, IC-2SD = 4.61), endophthalmitis (ROR = 178.27, IC-2SD = 6.70); brolucizumab-retinal vasculitis (ROR = 2930.41, IC-2SD = 7.47) and/or retinal artery occlusion (ROR = 391.11, IC-2SD = 6.10), dry eye (ROR = 12.48, IC-2SD = 2.88).

Conclusion: The presence of AEs should bring clinical attention. The use of anti-VEGF drugs should be based on the patient’s underlying or present medical condition to reduce any adverse event associated with the treatment.
Identification of highly effective inhibitors against SARS-CoV-2 main protease: From virtual screening to in vitro study
Authors: Wang Hu , Wen Jun , Yang Yang , Liu Hailin , Wang Song , Ding Xiaoli , Zhou Chunqiao , Zhang Xuelin
Published: November 2022

Background and Objective: The public’s safety has been significantly jeopardized by the pandemic of COVID-19, which is brought on by the highly virulent and contagious SARS-CoV-2 virus. Finding novel antiviral drugs is currently of utmost importance for the treatment of patients with COVID-19. Main protease (3CLpro) of SARS-CoV-2 is involved in replication of virus, so it is considered as a promising target. Using small molecules to inhibit SARS-CoV-2-3CLpro activity may be an effective way to prevent viral replication to fight COVID-19. Despite the fact that some SARS-CoV-2-3CLpro inhibitors have been described, only few of them have high levels of inhibition at nanomolar concentrations. In this study, we aimed to screen out effective SARS-CoV-2-3CLpro inhibitors.

Methods: To identify highly effective SARS-CoV-2-3CLpro inhibitors, a pharmacophore mapping and multiple-conformation docking were efficiently applied to find novel hit compounds from a database. Then, the stability of the 3CLpro-hit complexes was validated by using molecular dynamics simulation. Finally, biological assay was used to assess the inhibition effects of hit compounds on SARS-CoV-2-3CLpro.

Results: Four hit compounds were identified by using computer-assisted strategy. Molecular dynamics simulation suggested that these hits bound stably to the 3CLpro-active pocket. Bioassay showed that all the hits had potent inhibition against SARS-CoV-2-3CLpro with IC50 values in the range of 0.017–0.83 μM. Particularly, hit one was the best 3CLpro inhibitor and its inhibition effect of SARS-CoV-2-3CLpro (IC50 = 0.017 ± 0.003 µM) was about 236 times stronger than that of ML300 (IC50 = 4.01 ± 0.66 µM).

Conclusion: These data indicate that hit one could be regarded as an anti-SARS-CoV-2 candidate worth exploring further for the treatment of COVID-19.
Combined metabolomics and network pharmacology to elucidate the mechanisms of Dracorhodin Perchlorate in treating diabetic foot ulcer rats
Authors: Deng Pin , Liang Huan , Wang Shulong , Hao Ruinan , Han Jinglu , Sun Xiaojie , Pan Xuyue , Li Dongxiao , Wu Yinwen , Huang Zhichao , Xue Jiajia , Chen Zhaojun
Published: November 2022

Background: Diabetic foot ulcer (DFU) is a severe chronic complication of diabetes, that can result in disability or death. Dracorhodin Perchlorate (DP) is effective for treating DFU, but the potential mechanisms need to be investigated. We aimed to explore the mechanisms underlying the acceleration of wound healing in DFU by the topical application of DP through the combination of metabolomics and network pharmacology.

Methods: A DFU rat model was established, and the rate of ulcer wound healing was assessed. Different metabolites were found in the skin tissues of each group, and MetaboAnalyst was performed to analyse metabolic pathways. The candidate targets of DP in the treatment of DFU were screened using network pharmacology. Cytoscape was applied to construct an integrated network of metabolomics and network pharmacology. Moreover, the obtained hub targets were validated using molecular docking. After the topical application of DP, blood glucose, the rate of wound healing and pro-inflammatory cytokine levels were assessed.

Results: The levels of IL-1, hs-CRP and TNF-α of the Adm group were significantly downregulated. A total of 114 metabolites were identified. These could be important to the therapeutic effects of DP in the treatment of DFU. Based on the network pharmacology, seven hub genes were found, which were partially consistent with the metabolomics results. We focused on four hub targets by further integrated analysis, namely, PAH, GSTM1, DHFR and CAT, and the crucial metabolites and pathways. Molecular docking results demonstrated that DP was well combined with the hub targets.

Conclusion: Our research based on metabolomics and network pharmacology demonstrated that DP improves wound healing in DFU through multiple targets and pathways, and it can potentially be used for DFU treatment.
Integrated analysis of comprehensive metabolomics and network pharmacology to reveal the mechanisms of abelmoschus manihot (L.) medik. in the treatment of cisplatin-induced chronic kidney disease
Authors: Liao Jian-Cheng , Li Chang-Yin , Teng Feng-Meng , Jian-Chen  , Yu Jiang-Yi , Ju Wen-Zheng , Zou Jian-Dong
Published: November 2022

Background: Abelmoschus manihot (L.) Medik (“Huangkui” in Chinese, HK) has been widely used for the treatment of kidney diseases. Nephrotoxicity is the side effect of cisplatin (CDDP), which greatly limits its clinical application. Therefore, CDDP could be used to establish the chronic kidney disease (CKD) model. However, the protective effects of HK on CDDP-induced CKD have not been investigated.

Purpose: To explore the protective effect and underlying mechanisms of HK on multiple low-dose CDDP-induced CKD in rats by the integrated analysis of serum, kidney, and urine metabolomics and network pharmacology.

Methods: The CKD model was induced by multiple low-dose CDDP. Body weight, organ index, serum biochemical, and kidney histology were examined to evaluate the effect of HK. Serum, kidney, and urine were collected and profiled by HILIC/RPLC-Q-TOF/MS-based metabolomics. Potential biomarkers (PBs) were screened according to the criteria of VIP >1, p < 0.01, and FC > 2, and then identified or assigned. The pathway analysis and PBs enrichment were conducted by MetaboAnalyst and ChemRICH. Furthermore, network pharmacology was adopted to dig out the active components and targets. Finally, the results from metabolomics and network pharmacology were integrated to confirm each other.

Results: HK could recover the CDDP-induced abnormal pharmacological and metabolic profile changes. A total of 187 PBs were screened and identified from the serum, kidney, and urine metabolomics. Pathway analysis showed that multiple metabolic pathways, mainly related to amino acid and lipid metabolisms, were involved in the nephroprotective effect of HK, and especially, HK could significantly alleviate the disorder of tryptophan metabolism pathway in serum, kidney, and urine. Meanwhile, network pharmacology analysis revealed that 5 components in HK and 4 key genes could be responsible for the nephroprotection of HK, which also indicated that the metabolism of tryptophan played an important role in HK against CKD.

Conclusion: HK has a nephroprotection on CDDP-induced CKD, mainly by restoring the dysregulation of tryptophan metabolism. Integrated analysis of serum, kidney, and urine metabolomics and network pharmacology was a powerful method for exploring pharmacological mechanisms and screening active components and targets of traditional Chinese medicine.
Dissolvable hyaluronic acid microneedles loaded with β-Elemene for the treatment of psoriasis
Authors: Wang Chun , Hao Ruiqi , Peng Baowei , Chang Jiang , Chen Shisheng , Chen Yanxin , Yin Xiaohang , Que Yumei , Fan Chen , Xu Yuhong
Published: November 2022

The pathology of psoriasis involves the over-proliferation of keratinocytes, exaggerated inflammation of keratinocytes, and infiltration of inflammatory cells such as macrophages (Mø), etc. The therapeutic outcomes of current treatment targeting one single pathological process are less than satisfactory. Based on their diverse biological activities, natural products offer a potential solution to this problem. In this study, we investigated the effects of β-Elemene (ELE) on both psoriatic keratinocytes and M1-type Mø (M1-Mø) in vitro. Hyaluronic acid (HA) microneedles loaded with ELE (HA-ELE-MN) were also fabricated and tested for the treatment of psoriasis in vivo using an imiquimod (IMQ)-induced psoriatic mice model. Our data suggest that ELE induces apoptosis and inhibits inflammation of psoriatic keratinocytes. In addition, ELE attenuates the expression of inflammatory cytokines secreted from M1-Mø, thus indirectly inhibiting the inflammation of keratinocytes. Furthermore, HA-ELE-MN has been found to significantly alleviate symptoms in an IMQ-induced psoriatic mice model by inducing keratinocytes apoptosis, suppressing keratinocytes proliferation, and inhibiting M1-Mø infiltration. Taken together, this study demonstrates that ELE can be used for the treatment of psoriasis by targeting both keratinocytes and M1-Mø, which provides a potential novel reagent for psoriasis treatment.
Autophagy: An important target for natural products in the treatment of bone metabolic diseases
Authors: Li Zhichao , Li Dandan , Su Hui , Xue Haipeng , Tan Guoqing , Xu Zhanwang
Published: November 2022

Bone homeostasis depends on a precise dynamic balance between bone resorption and bone formation, involving a series of complex and highly regulated steps. Any imbalance in this process can cause disturbances in bone metabolism and lead to the development of many associated bone diseases. Autophagy, one of the fundamental pathways for the degradation and recycling of proteins and organelles, is a fundamental process that regulates cellular and organismal homeostasis. Importantly, basic levels of autophagy are present in all types of bone-associated cells. Due to the cyclic nature of autophagy and the ongoing bone metabolism processes, autophagy is considered a new participant in bone maintenance. Novel therapeutic targets have emerged as a result of new mechanisms, and bone metabolism can be controlled by interfering with autophagy by focusing on certain regulatory molecules in autophagy. In parallel, several studies have reported that various natural products exhibit a good potential to mediate autophagy for the treatment of metabolic bone diseases. Therefore, we briefly described the process of autophagy, emphasizing its function in different cell types involved in bone development and metabolism (including bone marrow mesenchymal stem cells, osteoblasts, osteocytes, chondrocytes, and osteoclasts), and also summarized research advances in natural product-mediated autophagy for the treatment of metabolic bone disease caused by dysfunction of these cells (including osteoporosis, rheumatoid joints, osteoarthritis, fracture nonunion/delayed union). The objective of the study was to identify the function that autophagy serves in metabolic bone disease and the effects, potential, and challenges of natural products for the treatment of these diseases by targeting autophagy.
Order and density fluctuations near the boundary in sheared dense suspensions
Authors: Miller Joia M. , Blair Daniel L. , Urbach Jeffrey S.
Published: November 2022

We introduce a novel approach to reveal ordering fluctuations in sheared dense suspensions, using line scanning in a combined rheometer and laser scanning confocal microscope. We validate the technique with a moderately dense suspension, observing modest shear-induced ordering and a nearly linear flow profile. At high concentration (ϕ = 0.55) and applied stress just below shear thickening, we report ordering fluctuations with high temporal resolution, and directly measure a decrease in order with distance from the suspension’s bottom boundary as well as a direct correlation between order and particle concentration. Higher applied stress produces shear thickening with large fluctuations in boundary stress which we find are accompanied by dramatic fluctuations in suspension flow speeds. The peak flow rates are independent of distance from the suspension boundary, indicating that they likely arise from transient jamming that creates solid-like aggregates of particles moving together, but only briefly because the high speed fluctuations are interspersed with regions flowing much more slowly, suggesting that shear thickening suspensions possess complex internal structural dynamics, even in relatively simple geometries.
Priming exercise accelerates oxygen uptake kinetics during high-intensity cycle exercise in middle-aged individuals with type 2 diabetes
Authors: Rocha Joel , Gildea Norita , O’Shea Donal , Green Simon , Egaña Mikel
Published: November 2022

Background: The primary phase time constant of pulmonary oxygen uptake kinetics (V·O2τp) during submaximal efforts is longer in middle-aged people with type 2 diabetes (T2D), partly due to limitations in oxygen supply to active muscles. This study examined if a high-intensity “priming” exercise (PE) would speed V·O2τp during a subsequent high-intensity cycling exercise in T2D due to enhanced oxygen delivery.

Methods: Eleven (4 women) middle-aged individuals with type 2 diabetes and 11 (4 women) non-diabetic controls completed four separate cycling bouts each starting at an ‘unloaded’ baseline of 10 W and transitioning to a high-intensity constant-load. Two of the four cycling bouts were preceded by priming exercise. The dynamics of pulmonary V·O2 and muscle deoxygenation (i.e. deoxygenated haemoglobin and myoglobin concentration [HHb + Mb]), were calculated from breath-by-breath and near-infrared spectroscopy data at the vastus lateralis, respectively.

Results: At baseline V·O2τp, was slower (p < 0.001) in the type 2 diabetes group (48 ± 6 s) compared to the control group (34 ± 2 s) but priming exercise significantly reduced V·O2τp (p < 0.001) in type 2 diabetes (32 ± 6 s) so that post priming exercise it was not different compared with controls (34 ± 3 s). Priming exercise reduced the amplitude of the V·O2 slow component (As) in both groups (type 2 diabetes: 0.26 ± 0.11 to 0.16 ± 0.07 L/min; control: 0.33 ± 0.13 to 0.25 ± 0.14 L/min, p < 0.001), while [HHb + Mb] kinetics remained unchanged.

Conclusion: These results suggest that in middle-aged men and women with T2D, PE speeds V·O2τp likely by a better matching of O2 delivery to utilisation and reduces the V·O2 As during a subsequent high-intensity exercise.
Increased CD34 in pancreatic islet negatively predict islet β-cell decrease in type1 diabetes model
Authors: Huang Shichen , Li Zhiyuan , Sun Yuhan , Chen Baiyi , Jiang Yuxin , Hong Feng
Published: November 2022

Islet β-cell biomarkers can reflect changes in the number and function of islet β-cells in the prediabetes or early diabetes stage. CD34 is a commonly used stem cell biomarker; however, its expression and function in pancreatic islets remain unclear. In the present study, double immunofluorescence staining, proteomic bioinformatics analysis, and correlation analysis were used to explore the potential of CD34 as an islet β-cell biomarker. Bioinformatics analysis revealed that the amino acid sequence of CD34 was conserved among multiple species and abundantly expressed on mouse and human pancreatic tissues. Immunofluorescence demonstrated that in the control rat pancreas, CD34 was expressed on glucagon-labeled islet α-cells but not on insulin-labeled islet β-cells. Furthermore, the proportion of CD34-positive cells, which were also positive for glucagon, was significantly increased in alloxan-induced diabetes models. Statistical analysis revealed that the expression of CD34 was negatively correlated with the number of insulin-labeled islet β-cells during diabetes progression in dose-dependent fashion in alloxan-induced diabetes models. Furthermore, the results suggested that the transdifferentiation of islet β-cells into islet α-cells may occur in the process of diabetes. Thus, the present study demonstrated that CD34 is expressed on islet α-cells, and its number is linearly and negatively correlated with the number of islet β-cells, suggesting that CD34 can be used as a prospective biomarker for islet β-cells in the early diagnosis of diabetes. The study also suggests the transformation of β-cells to α-cells in diabetes which provide a potential to be applied towards diabetes mechanism research.
Genome-wide characterization of PEBP gene family in Perilla frutescens and PfFT1 promotes flowering time in Arabidopsis thaliana
Authors: Xu Huaxiang , Guo Xi , Hao Youjin , Lu Geng , Li Dan , Lu Junxing , Zhang Tao
Published: November 2022

Phosphatidylethanolamine-binding proteins (PEBP) family plays important roles in regulating plant flowering time and morphogenesis. However, geneme-wide identification and functional analysis of PEBP genes in the rigorous short-day plant Perilla frutescens (PfPEBP) have not been studied. In this study, 10 PfPEBP were identified and divided into three subfamilies based on their phylogenetic relationships: FT-like, TFL1-like and MFT-like. Gene structure analysis showed that all PfPEBP genes contain 4 exons and 3 introns. Motifs DPDxP and GIHR essential for anion-binding activity are highly conserved in PfPEBP. A large number of light-responsive elements were detected in promoter regions of PfPEBP. Gene expression of PfFT1 exhibited a diurnal rhythm. It was highly expressed in leaves under the short-day photoperiod, but higher in flowers and seeds under the long-day photoperiod. Overexpression of PfFT1 in Arabidopsis thaliana not only promoted early flowering of Col-0 or Ler, but also rescued the late flowering phenotype of ft-1 mutant. We concluded that PfFT1 promotes early flowering by regulating the expression of flowering-related genes AtAP1, AtLFY, AtFUL and AtSOC1. In conclusion, our results provided valuable information for elucidating the functions of PfPEBP in P. frutescens and shed light on the promoting effect of PfFT1 on flowering.
Frequent spontaneous structural rearrangements promote rapid genome diversification in a Brassica napus F1 generation
Authors: Orantes-Bonilla Mauricio , Makhoul Manar , Lee HueyTyng , Chawla Harmeet Singh , Vollrath Paul , Langstroff Anna , Sedlazeck Fritz J. , Zou Jun , Snowdon Rod J.
Published: November 2022

In a cross between two homozygous Brassica napus plants of synthetic and natural origin, we demonstrate that novel structural genome variants from the synthetic parent cause immediate genome diversification among F1 offspring. Long read sequencing in twelve F1 sister plants revealed five large-scale structural rearrangements where both parents carried different homozygous alleles but the heterozygous F1 genomes were not identical heterozygotes as expected. Such spontaneous rearrangements were part of homoeologous exchanges or segmental deletions and were identified in different, individual F1 plants. The variants caused deletions, gene copy-number variations, diverging methylation patterns and other structural changes in large numbers of genes and may have been causal for unexpected phenotypic variation between individual F1 sister plants, for example strong divergence of plant height and leaf area. This example supports the hypothesis that spontaneous de novo structural rearrangements after de novo polyploidization can rapidly overcome intense allopolyploidization bottlenecks to re-expand crops genetic diversity for ecogeographical expansion and human selection. The findings imply that natural genome restructuring in allopolyploid plants from interspecific hybridization, a common approach in plant breeding, can have a considerably more drastic impact on genetic diversity in agricultural ecosystems than extremely precise, biotechnological genome modifications.
Qualified foreign institutional investors and corporate innovation: From the perspective of corporate governance
Authors: Wang Xiao , Wang Wanting , Shi Xiang yan
Published: November 2022

Whether qualified foreign institutional investors can effectively play a governance role in the capital market and guide the transformation of corporate innovation from “high-volume and low-quality” to “high-volume and high-quality” is an important issue in the process of foreign capital introduction at the present stage. From the perspective of how QFII shareholding affects the innovation model of firms, this study analyzes the data of China’s A-share listed companies from 2007 to 2018 and finds that the shareholding of qualified foreign institutional investors has significantly improved the innovation level of the invested firms, which is reflected in the increase of innovation output and the improvement of innovation quality. The mediating effect shows that QFII shareholding can improve the innovation level of corporates by slowing down insider tunneling of holding companies and increasing the number of analysts to follow, which indicates that QFII is conducive to improving the governance structure of listed companies and improving their qualities. Further research finds that QFII shareholding has a positive impact on corporate efficiency by improving the level of corporate innovation. The above conclusions provide experience and reference for China to further introduce foreign capital.
Cognitive flexibility in younger and older children who stutter
Authors: Paphiti Maria , Eggers Kurt
Published: November 2022

Purpose

Recent research findings suggest possible weaknesses in cognitive flexibility (CF) in children who stutter (CWS) when compared to children who do not stutter (CWNS). Studies so far, have been conducted with either younger (3–6 years old) or older children (6–12 years old) with a variety of measures. The purpose of the present study was to investigate CF with the use of a single behavioral measure across a broader age range (4–10 years old).

Methods

Participants were 37 CWS (mean age = 6.90 years) and 37 age-and gender-matched CWNS (mean age = 6.88 years), divided in a younger (below 7 years) and older (above 7 years) age group. All participants undertook a computerized visual set-shifting task consisting of three blocks. CF was evaluated through across-and within-block comparisons of the actual response speed and accuracy values. In addition, mixing-and set-shifting-costs were evaluated based on the mean response speed and accuracy.

Results

All participants showed expected mixing-and set-shifting-costs. Only the within-block analyses yielded significant between (sub)group differences. Investigation of the block × classification group × age group interactions showed that older CWS had larger set-shifting-costs (slowed down more and made more errors) compared to older CWNS.

Conclusion

While all participants required more time during set-shifting trials, only the older CWS (7–10 years old), and not younger CWS, were slower and made more errors. This finding corroborates previous findings in CWS of a similar age and could possibly point to a role of CF in stuttering persistence.
Nurses’ feeling trusted and knowledge hiding: The role of psychological safety, felt obligation and traditionality
Authors: Lu Guangli , Liang Yipei , Ding Yueming , Tang Haishan , Zhang Yiming , Huang Haitao , Chen Chaoran
Published: November 2022

Knowledge hiding is one of the dilemmas of organizational knowledge management. For nurses, knowledge hiding behavior is not conducive to improving the quality and efficiency of their work and hinders the innovation of nursing services. Based on the social exchange theory, the current study constructed a moderated mediation model by taking psychological safety and felt obligation as mediating variables, and traditionality as moderating variable, and explored the mechanism of feeling trusted affecting knowledge hiding behavior. The empirical research based on 285 nurses from China shows that feeling trusted is negative correlate with knowledge hiding behavior; feeling trusted can negatively affect knowledge hiding by enhancing psychological safety and felt obligation; traditionality can positively moderate the relationship between feeling trusted and felt obligation, and feeling trusted has a stronger positive influence on felt obligation of highly traditional nurses; traditionality has no significant moderating effect between feeling trusted and psychological safety. Theoretically, this study supplements the influencing factors of knowledge hiding, examines the complex mechanism between feeling trusted and knowledge hiding and supplements the boundary conditions for feeling trusted to play its role from the perspective of individual characteristics (i.e., traditionality). From the perspective of practical implication, this study suggests that managers should pay attention to using trust strategies to enhance subordinates’ psychological safety and felt obligation, especially for highly traditional nurses, thus reducing knowledge hiding.
Influence of different playing styles among the top three teams on action zones in the World Cup in 2018 using a Markov state transition matrix
Authors: Liu Tianbiao , Zhou Chenye , Shuai Xumei , Zhang Li , Zhou Jingjing , Yang Lang
Published: November 2022

Purpose

In football, attacking has seen evolving for decades and attacking pattern detection is an important topic in this sport. The purpose of this study was to identify the general and threatening attacking patterns of different playing styles in world top football matches, which represented the latest evolvement of soccer attacking.

Methods

Attacking sequence data of the top three teams from 21 matches in the 2018 World Cup were collected. The three teams were classified into two playing styles according to a previous study, France was a direct-play team, and Croatia and Belgium were possession-play teams. The football field was divided into 12 zones and Markov transition matrix-based zone models were applied to assess the attacking pattern in the 21 matches. Both descriptive analysis and simulative analysis were conducted using this model.

Results

The results revealed that (1) flanker attacks were frequently taken among all three teams, and possession playing teams (Croatia and Belgium) played more often than direct playing teams (France) in their center of the midfield zone and (2) forward passes across/through zones toward the middle of attacking quarter (A1/4) have a positive impact of creating a chance of a goal.

Conclusion

Using Markov transition matrix, general and threatening attacking patterns were found. The combination of possession play and counterattack was a new trend that emerged in the 2018 World Cup. These findings can help coaches to develop corresponding strategies when facing opponents of different playing styles.
Shared and nonshared agency for occupational goals with mothers, fathers, VIPs, and romantic partners during young adulthood
Authors: Chang Esther S. , Shane Jacob , Villarreal Brandilynn , Heckhausen Jutta
Published: November 2022

This study applied a framework of shared and nonshared agency to investigate how social partners can help and hinder young adults’ career development. We also considered the extent to which motivational control could be promoted or burdened when young people seek help and encouragement from others in their careers. Based on the importance of shared agency in life goal pursuit, it was hypothesized that shared agency (i.e., perceived support and collaboration) with mothers, fathers, important adults, and romantic partners would have direct and positive associations with young adults’ career satisfaction and exploration and positive indirect associations on career development via motivational control. We further hypothesized that nonshared agency (i.e., directing and uninvolvement) would have direct and negative effects on career satisfaction and exploration and negative indirect effects on career development via motivational strategies. Results indicated that relationships can facilitate career development but differently depending upon relationship type. We found that support and directing from mothers and VIPs had positive associations with outcomes via individual motivational control whereas a total effect of collaboration with fathers and romantic partners were associated with outcomes without an indirect effect via motivational control. These findings are discussed within the context of previous socialization research and theory.
How do you see your role as a follower? A quantitative exploration of followers’ role orientation
Authors: Gesang Elena
Published: November 2022

How people see and define their role in different social settings has been of interest in psychological science for several decades. However, followers’ role orientations, followers’ beliefs about their role in the workplace, and how they execute their roles, have mostly been omitted in research so far. Nevertheless, followers’ role orientations are important as they can affect (work-related) behavior. Therefore, this study quantitatively investigates the structure, heterogeneity, and consistency of followers’ role orientations as well as the role orientations’ link to work-related traits and behaviors. For this purpose, content and statistical analysis of an Implicit-Followership-Theory Scale and latent profile analyses were conducted with data from two points in time via a sample of German employees (t1: n = 211, t2: n = 69). The results indicate that a passive/active work attitude (Enthusiasm) plays the most prominent part in differences in followers’ role orientation, followed by a positive/negative work ethic (Industry), and cooperativeness toward the leader (Good Citizen). Moreover, followers can be differentiated according to their role orientations into three distinct types: the Anti-Prototype, the Moderate Anti-Prototype, and the Moderate Prototype. Followers of the Moderate Prototype have the highest values in work-related traits and behaviors like conscientiousness and personal initiative. Followers’ profile affiliation is stable for three-quarters of the sample over 4–6 weeks. Overall, these findings point to role orientation being a (performance-related) follower characteristic, making role orientations relevant for application processes, especially for positions with frequent leader–follower interactions. Lastly, results show a current conceptual inaccuracy. They indicate the need to differentiate more rigorously between role orientations and Implicit Followership Theories (IFT), although currently both are often used synonymously. Therefore, recommendations for alterations to the used Implicit Followership Theory scale for capturing followers’ role orientations are given, including the elimination of items with situational character and changes in wording and factor structure.
Intervention initiatives to raise young people’s interest and participation in STEM
Authors: Schneider Barbara , Chen I-Chien , Bradford Lydia , Bartz Kayla
Published: November 2022

For nearly a decade, two science interventions anchored in project-based learning (PBL) principles have been shown to increase student science learning in 3rdgrade and high school physical science classes. Both interventions employed a randomized control trial of several thousand students (N = 3,271 in 3rdgrade and N = 4,238 in 10th, 11th, and 12thgrades). Incorporating a rich background of research studies and reports, the two interventions are based on the ideas of PBL as well as the National Academies of Science’s publications, including how children learn; how science learning and instruction can be transformed; and the performance expectations for science learning articulated in the Next Generation of Science Standards. Results show significant positive increases in student academic, social, and emotional learning in both elementary and secondary school. These findings can be traced, in part, to carefully crafted experiential participatory activities and high-quality instructional materials which act as strong facilitators for knowledge acquisition and use. Reviewing the innovations undertaken by these two interventions, this article describes the importance of studying social and emotional factors ‘in situ’, using the Experience Sampling Method (ESM), that can motivate and engage students in science learning in both elementary and secondary school. Using these ‘in situ’ data collection (N = 596 students in 3rdand N = 1412 students in 10th, 11th, and 12thgrades) along with case studies and repeated measures analysis gave deep insights into emotional and social development for young children and adolescents. These methods should continue to be considered when trying to understand key factors of improving engagement in science.