bluuebunny/crossref_metadata_2025 huggingface dataset:
Morphological features of the eyelid margin in relation to conjunctival goblet cells and MUC5AC in tear film and clinical tests of dry eye
Authors: Dou Zexia , Sun Ming , Huang Yue , Zhao Shaozhen
Published: January 2024

Purpose This study aimed to investigate the morphological features of eyelid margin in dry eye patients and determine their correlations with conjunctival goblet cells, MUC5AC secretion, and clinical tests of tear film.
Methods Patients diagnosed with dry eye disease (DED) were asked to complete the evaluations of symptoms and signs of dry eye, including Ocular Surface Disease Index (OSDI) questionnaire, Lipid layer thickness (LLT), partial blinking (PB) rate, tear-film breakup time (TBUT), Schirmer’s I test (SIt), and meiboscore. Marx line (ML), lid wiper epitheliopathy (LWE), and meibomian orifices (MOs) were used as indicators of morphological features of the eyelid margin. PAS staining of conjunctival goblet cells and MUC5AC measurement of the tear samples were also performed.
Results A total of 30 patients diagnosed with DED were included. The ML score was correlated with the SIt. The MOs showed a significant negative correlation with the TBUT and LLT. The LWE was corrected to TBUT and meiboscore, and also associated with decreased conjunctival goblet cell density and MUC5AC concentration in the tear film rather than ML and MOs in the eyelid margin of dry eye patients. The tear MUC5AC concentration had a significant positive correlation with conjunctival goblet cell density.
Conclusion The eyelid margin is crucial for maintaining the integrity of tear film and ocular surface. Attracting more attention to the eyelid margin is beneficial to routine clinical practice, and lid wipers could be used to evaluate the secretion of MUC5AC in the tear film secreted by conjunctival goblet cells.
The study of human mRNA m7G methylation writer molecule related lncRNA in gastric cancer
Authors: Ma Tianyu , Zhou Dongli , Hu Binbin
Published: January 2024

OBJECTIVE
Presenting a poor prognosis, gastric cancer (GC) remains one of the leading causes of disease and death worldwide. N7-methylguanosine (m7G) is the most prevalent RNA modification. m7G-long noncoding RNAs (lncRNAs) regulate GC onset and progression, but their precise mechanism in GC is unclear.
MATERIALS AND METHODS
The Cancer Genome Atlas database helped extract gene expression data and clinical information for GC. Coexpression analysis helped identify m7G-related lncRNAs. Univariate Cox and multivariate Cox analyses helped identify m7G-related lncRNAs with predictive capability. The chi square test was used to study the relationship between the expression of lncRNAs and clinical characteristics. Enrichment analysis (GSEA) was used for pathway enrichment analysis.
RESULTS
Thirty-one m7G lncRNAs were identified by coexpression analysis. Further univariate Cox and multivariate Cox analyses were used to screen the prognostic lncRNAs. There is only one lncRNA, REPIN1 antisense RNA 1 (REPIN1-AS1), that can be used as an independent prognostic factor. Therefore, REPIN1-AS1 was selected as the molecule for further study. Then, REPIN1-AS1 was enriched by GSEA. Finally, drug sensitivity was analysed.
CONCLUSIONS
We constructed a m7G-related lncRNA prognostic model, and the results showed that REPIN1-AS1 could be an independent prognostic factor involved in tumour formation and other signalling pathways and had potential therapeutic targets.
VERSA: Validating Evaluations with a Robust Scoring Approach - Detecting Fake Reviews through MRCS
Authors: Ennaouri Mohammed , Zellou Ahmed
Published: January 2024

The emergence of online commerce in the digital age resulted to an unparalleled dependence on consumer feedback for making informed choices. However, the rise in the importance of reviews has coincided with an increase in fraudulent behavior, as fake reviews have come into platforms and influenced users' opinions. The widespread use of these deceptive reviews has become an increasing issue, reducing the trust that is essential for the success of online marketplaces.
This study uses a comprehensive approach to handle the difficulty of identifying fake reviews. This distinctive strategy introducing a multidimensional methodology incorporating advanced Natural Language Processing (NLP) tools. The utilization of Word2Vec for semantic richness in review analysis is detailed, emphasizing its ability to capture contextual complexities. The integration of new metric navigates the semantic landscape, offering a normalized measure of textual closeness critical for precise detection. A distinctive feature is the incorporation of an exaggeration detection method, which improves the model's capacity to reveal misleading complexities in reviews' language. Moreover, an adaptive clustering is elaborated, which deviates from conventional K-means clustering by continuously adjusting clusters to account for the dynamic nature of deceptive language patterns.
The approach’s excellent accuracy is demonstrated via empirical validation on the Yelp Labeled Dataset, which also highlights areas for development. Metrics like precision, recall, and F1 score provide light on the model's advantages and direct further improvements. The conclusion examines how misleading reviews affect digital trust more broadly and highlights the proposed approach as a critical first step in reducing fraudulent activity.
Study of short-term periodicities in the occurrence of Forbush decreases: Wavelet analysis
Authors: Singh Y. P. , Badruddin Badruddin
Published: January 2024

We utilized the Forbush decreases (magnitude >1.5%) detected in cosmic ray neutron monitor data during continuous five solar cycles, viz., 20, 21, 22, 23 and 24 (1965 to 2019) and subjected them to wavelet analysis in order to obtain the possible periodicities in their occurrence. We also studied the periodicities separately during the odd and even solar activity cycles. In addition to solar activity, the solar magnetic polarity and its extension into the interplanetary space makes significant difference in the cosmic ray modulation in the helisphere, we have also applied the wavelet analysis procedure separately during positive (A > 0) and negative (A < 0) polarity states of the heliospheric magnetic fields. Observed periodicities in Forbush decreases have been discussed and compared with earlier detected periodicities in solar and geomagnetic activity indices, e.g., sunspot numbers, sunspot areas, sunspot groups, solar flares, coronal mass ejections, and various geomagnetic activity indices. Significant short-term periodic behaviour detected in the occurrence of Forbush decreases, which in general, corroborates the observed behaviour in solar (in particular, solar eruptive activity) and geomagnetic activity. Understanding the quasi-periodic process in magnetic field emergence from solar active regions and solar eruptive activity, as well as solar-terrestrial coupling and space weather effects, requires comparing the quasi-periodic behaviour between parameters representing solar and geomagnetic activity along with cosmic ray variability.
Comprehensive analysis of LD-related genes signature for predicting prognosis and immunotherapy response in clear cell renal cell carcinoma
Authors: Yangtao Jia , Xinke Dong , Fangzheng Yang , Huimin Long , Libin Zhou
Published: January 2024

Background
Lipid droplets (LD) in renal clear cell carcinoma (ccRCC)play a crucial role in lipid metabolism and immune response modulation. The purpose of this study was to create a LD-related signature to predict prognosis and guide the immunotherapy and targeted therapy in ccRCC patients.
Methods
We conducted a comprehensive analysis using transcriptional profiles and clinical data obtained from The Cancer Genome Atlas (TCGA). LD-related genes were identified from existing literature and the GeneCards database, and differentially expressed genes were determined. Sequentially, we conducted Cox regression analysis and Lasso regression analysis, to establish a prognostic risk model. The performance of the risk model was evaluated using Kaplan-Meier (KM) analysis and time-dependent receiver operating characteristic (ROC) analysis. Additionally, gene set enrichment analysis (GSEA), ESTIMATE, CIBERSORT, and immunophenoscore (IPS) algorithm were used to assess the tumor microenvironment (TME) and treatment response.
Results
We constructed a risk signature with four LD-related genes in the TCGA dataset, which could be an independent prognostic factor in ccRCC patients. Then, patients were classified into two risk groups and exhibited notable differences in overall survival (OS), progression-free survival (PFS), and TME characteristics. Furthermore, we developed a comprehensive nomogram based on clinical features, which demonstrated good prognostic predictive value. According to the results of GSEA analysis, immune-related pathways were found to be significantly enriched in the high-risk group. Additionally, the high-risk group displayed high levels of immune cell infiltration, TMB and IPS scores, indicating better efficacy of immune checkpoint inhibitors (ICIs). Finally, high-risk demonstrated reduced IC50 values compared to the low-risk counterpart for specific targeted and chemotherapeutic drugs., suggesting that the patients receiving these targeted drugs in high-risk group had better treatment outcomes.
Conclusions
Our LD-related genes signature provides reliable prognostic predictions and can guide immunotherapy and targeted therapy in ccRCC patients.
Safety Diagnostic Tool for Non-Small Cell Lung Cancer (NSCLC) Lyophilized Serum
Authors: Mohammed Mohammed , Mohammed Asmaa M S
Published: January 2024

Background and Objectives
The international protocol used to diagnose non-small cell lung cancer (NSCLC) usually faces an inappropriate result due to the poor diagnostic ability in the early stages. Carcinoembryonic Antigen (CEA), an established serum tumor marker that is used for NSCLC diagnosis, has limited sensitivity and specificity, but, still, it is the predominant complementary detecting tool wherein its results confirm diagnostic radiology findings (PET-CT). Unfortunately, the limited range of its sensitivity is unable to classify approximately one third of patients suffering from NSCLC. Due to a huge number of patients lately classified as NSCLC, the efficacy of the offered treatment is limited. Hence, the importance of discovering, improving, and establishing a new technique that participates in the NSCLC diagnosis is indeed urgent.
Methods
The low angle x-ray scattering (LAXS) technique was applied on the lyophilized serum of NSCLC patients to create patient profiles that were able to distinguish the molecular differences between NSCLC patients, avoiding the undesirable radiation exposure to the patients.
Results
The created LAXS profile was characterized by two peaks. The first scattering peak at 4.8° was sensitive to molecular alterations in protein structures that were the main characteristic differences from the normal serum. Comparing the measurements of LAXS profiles of NSCLC with the normal sera, the unique first scattering peak at 4.8° was elucidated as a characterization shape and profile for NSCLC and normal individuals. Conclusions: Using the LAXS technique gives us full details at a molecular level that are introduced as a promising tool that could be a supporter in NSCLC early detection.
On the characteristics of aphantasia, hyperphantasia and sensory imagery in a multi-cultural sample
Authors: Bruder Jennifer , Zehra Masooma
Published: January 2024

Sensory imagery, such as visual imagery, is assumed to be a universal cognitive ability, yet a current research gap is a lack of cultural diversity in the existing literature. Further, research has primarily focused on visual imagery, as opposed to other sensory imagery. Visual imagery presents with a large degree of individual variability and includes two extreme forms, aphantasia and hyperphantasia. This study investigated sensory imagery in a multi-cultural sample. Participants (N = 636) completed the Vividness of Visual Imagery Questionnaire (VVIQ) scale and the Plymouth Sensory Imagery Questionnaire (Psi-Q). Altogether 5.0% of participants fell within the aphantasia range (VVIQ: 16–32) and 12.1% within the hyperphantasia range (VVIQ: 75–80). Across the sample, no VVIQ differences were found for gender, age, occupation, or education. VVIQ scores positively correlated with all imagery scales in the Psi-Q, suggesting imagery across senses is not independent. To explore cultural differences, participants were divided into five cultural groups: 1) Western Educated Industrialized Rich & Democratic (WEIRD) (n = 89); 2) South-East Asia (SEA, n = 121); 3) South Asia (n = 180); 4) Middle East and North Africa (MENA, n = 86), and 5) Arabian Gulf (Arab, n = 43). WEIRD and SEA participants reported significantly higher levels of visual imagery in the VVIQ than those from MENA. Within the Psi-Q sensory subscales, SEA and WEIRD participants reported significantly higher imagery than MENA and Arabian Gulf participants. In conclusion, this study contributes to previous prevalence reports for aphantasia and hyperphantasia in a novel sample and demonstrates the need to consider culture when investigating imagery across all senses.
Effect of Humidity on of Sol-Gel Glass Chirowaveguide Thin Films and Microstructures
Authors: SAHIB Hoshang , Bensalah-Ledoux Amina , Gassenq Alban , Baguenard Bruno , Guy Laure , Guy Stephan
Published: January 2024

We present a comprehensive study on the effect of humidity on organically modified sol-gel glasses (ormosils) thin films and related-microstructures, using optical characterization methods.Using a Mach-Zehnder interferometer and a polarimetric setup, we quantitatively measure the variations in refractive index and linear birefringence induced by changes in ambient relative humidity. Our findings unveil the high sensitivity of the material's refractive index to humidity, predominantly attributed to the hygroscopic nature of the polyethylene glycol (PEG) component embedded within the ormosils.Furthermore, We show that stress-induced birefringence during the deposition of the sol-gel layers is partially released in the structured layers compared to the planar one. The remaining stress is reduced by the effect of humidity in a linear relationship in which the proportionality factor is higher in the planar layers. The results shown here shed new light on the complex relationship between humidity and sol-gel derived materials and contribute to the understanding of the mechanisms behind their sensitivity to humidity.
The January 1 st 2024 Noto Peninsula Co-seismic Landslides Hazards: Preliminary Results
Authors: Gomez Christopher
Published: January 2024

On the first day of 2024, a strong Mw.7.6 earthquake followed by a tsunami shook the Noto Peninsula (Japan) located on the coast facing the Sea of Japan. It resulted in numerous casualties, infrastructures and dwelling destroyed. The earthquake also triggered at least 930 coastal and mountain co-seismic landslides that were digitized from the emergency aerial photographs (2/1/2024), in order to provide a preliminary assessment of their distribution and characteristics. The medium surface of the landslides was found to be 1749 m2, with numerous small < 50 m2 landslide and at least one large deep-seated landslide (0.8 km x 1 km). The mountain landslides were concentrated around two clusters, which were not close to the epicentre, but around 7 km and 10 km from the epicentre. From a disaster perspective, the 1/1/2024 Noto Peninsula earthquake is typical of a ‘coastal earthquake’ where the coastal landslides, even sparse collapsed on the main artery of the peninsula, the ring road, isolating communities and hampering the disaster relief process.
Contemporary Survival Outcomes and Prognostic Factors in Glioblastoma: An Institutional Analysis
Authors: Narang Kushal , Kataria Tejinder , Bisht Shyam Singh , Gupta Deepak , Banerjee Susovan , Mayank Mayur , Shishak Sorun , Kaliyaperumal Venkatesan , Tamilselvan S , Kamaraj Dayanithi , Abraham Susan
Published: January 2024

Purpose
There have been significant advancements in diagnostic radiology and pathology, and in the technical aspects of surgery, radiation therapy and temozolomide used for treatment of glioblastoma. We analyzed the outcomes of 267 adult patients with glioblastoma, consecutively treated between December 2010 and November 2018 using modern techniques at our institute.
Methods
All patients underwent surgical resection, volumetric modulated arc therapy-based radiation therapy using institute-specific target-delineation guidelines and image-guidance, and temozolomide according to Stupp’s protocol. Serial multiparametric magnetic resonance imaging-based follow-up ensured early detection of disease progression. Appropriate salvage therapy was determined based on clinicopathological attributes. Kaplan-Meier survival plots, log-rank test and Cox regression analysis was performed on the prospectively recorded dataset to estimate survival, and the factors affecting it.
Results
The median progression-free survival (PFS), 1-year PFS, and 2-year PFS were 10 months, 37.8%, and 17.5%, respectively. Radiation dose ≥ 54 Gy and ≥ 4 adjuvant TMZ cycles were associated with favourable PFS. The median overall survival (OS), 2-year OS and 5-year OS were 24 months, 48% and 18%, respectively. One-year disease control, surgery-use at progression, and systemic therapy-use at progression were associated with favourable OS. Haematological toxicity ≥ Grade 2 was evident in 6% patients during concurrent radiation-temozolomide phase, and in 9% patients in adjuvant temozolomide phase. Post-radiation neurocognitive deficits were noted in 20.1% patients, with onset at a median duration of 10 months.
Conclusion
Modern diagnostic and therapeutic techniques for glioblastoma effected a near-doubling of survival compared to historical data, with acceptable late toxicity.
B-Index as activity proxy for Proxima Centauri star ; HARPS survey
Authors: Azizi Fatemeh , Mirtorabi Mohammad Taghi , Foroughi Rahimeh
Published: January 2024

Stellar activity cycles on magnetically active stars can be estimated by molecular absorption bands.A new color indicator which compares absorptional line strength of the T iOλ567nm with its near continuum has previously been introduced. In this work we use this indicator to evaluation activity cycle for Proxima Centauri star, using spectroscopic data from HARPS survey. The results indicate periodicity with an activity period of 2873+47.4 −53.9 days.
Continual Learning Approaches to Hand-Eye Calibration in Robots
Authors: Bahadir Ozan , Siebert Jan Paul , Aragon-Camarasa Gerardo
Published: January 2024

This study addresses the problem of hand-eye calibration in robotic systems by developing a continuous learning (CL)-based approach. Traditionally, robots require explicit models to transfer knowledge from camera observations to their hands or base. However, this poses limitations, as the hand-eye calibration parameters are typically valid only for the current camera configuration. To overcome this, we propose a flexible and autonomous hand-eye calibration system that can adapt to changes in camera pose over time.

Three CL-based approaches are introduced: the naive CL approach, the reservoir rehearsal approach, and the hybrid approach, combining reservoir sampling with new data evaluation. The naive CL approach suffers from catastrophic forgetting, while the reservoir rehearsal approach mitigates this issue by sampling uniformly from past data. The hybrid approach further enhances performance by incorporating reservoir sampling and assessing new data for novelty.

Experimental evaluations conducted in simulated and real-world environments demonstrate that the CL-based approaches, except for the naive one, achieve competitive performance compared to traditional batch learning-based methods. This suggests that treating hand-eye calibration as a time sequence problem enables the extension of the learned space without complete retraining. The adaptability of the CL-based approaches facilitates accommodating changes in camera pose, leading to an improved hand-eye calibration system.
Application of Full Waveform Inversion in passive seismic explorations: Some practical considerations
Authors: kashi Mohammad Hazrati , Tatar Mohammad , Norouzi Siavash
Published: January 2024

Passive seismic tomography has been proved to be a great alternative solution for areas which is highly challenging to be explored by active seismic methods. As a modern high resolution tomographic algorithm, we consider Full Waveform Inversion (FWI) as a potential passive seismic method for Dehdasht embayment, South West, Iran. However, before the implementation of FWI process on the real dataset of Dehdasht area, we test our proposed multi-scale workflow on a synthetic model resembling our area of interest. Using this workflow, different factors affecting the FWI results such as source and receiver configurations, presence of noise in data and erroneous source location were evaluated. Fortunately, with a receiver spacing similar to Dehdasht acquisition network, using enough number of sources and especially in Vs model, it is possible to reconstruct such high-resolution features of the medium as hydrocarbon trap, high-velocity bedrock and shallow syncline structure. Moreover, despite a high level of random noise added to the data, the results demonstrate that our proposed workflow is robust enough to diminish the adverse effect of noise and present a clear subsurface tomographic image of the medium. However, simulations with erroneous source locations warns about the divergence and instability of FWI process caused by the sources with high location errors. As a result, before the implementation of FWI on the real dataset of Dehdasht project, we should dedicate a specific attention on source relocation or source modeling process in general. At the end, we propose a hierarchical processing workflow for the real dataset of Dehdasht to guarantee the convergence to a high-resolution tomographic model of the Dehdasht embayment area.
In vitro effect of Granulocyte-macrophage colony-stimulating factor (GM-CSF) on the expression of related genes to sperm motility and energy metabolism, and ICSI outcomes in obstructive azoospermic patients
Authors: Sabz fatemeh Tanhaye Kalate , Hosseini Elham , Amjadi Fatemeh Sadat , Mohammadian Masoud , Zandieh Zahra , Mohammadian Farnaz , Kafaeinezhad Raheleh , Ashrafi Mahnaz
Published: January 2024

Background
Granulocyte-macrophage colony-stimulating factor (GM-CSF) expressed in the human reproductive system, holds a pivotal role in the reproductive processes. This study investigates the in vitro effect of GM-CSF on the testicular sperm of obstructive azoospermia (OA) patients and assesses the effectiveness of GM-CSF‐supplemented sperm media in Intracytoplasmic sperm injection (ICSI) outcomes.
Methods and Results
Following testicular sperm extraction from 20 patients diagnosed with OA, each sample was divided into two parts: the experimental samples were incubated with the medium containing 2 ng/ml GM-CSF at 37°C for 60 min, and control samples were incubated with medium without GM-CSF. Subsequently, the oocytes retrieved from the partner were injected with sperms from treatment and the control groups. The sperm parameters ( motility, viability), the expression level of sperm motility-related genes (PIK3R1, PIK3CA, and AKT1 ), and sperm energy metabolism-related genes (GLUT1, GLUT3, and GLUT14) were assessed. Furthermore, the fertilization and cleavage rates and embryo quality were evaluated. Supplemented testicular sperm with GM-CSF significantly increased motility parameters, the mRNA expression of PIK3R1, AKT1, and GLUT3 compared to the non-treated group (p < 0.05). However, no significant differences in mRNA expression of PIK3CA, GLUT1, or GLUT14 were identified. Based on ICSI outcomes, the GM-CSF treatment group exhibited significantly higher fertilization rates (p = 0.027), cleavage rates (p = 0.001), and the proportion of good-quality embryos (p = 0.002) compared to the control group.
Conclusions
GM-CSF increased gene expression related to motility and energy metabolism pathway and effectively had a positive effect on the motility of testis-extracted spermatozoa and, consequently yielding positive clinical outcomes.
The dynamics of vertical coalescence of acoustically levitated droplets
Authors: Vashi Aditya , Yadav Ajeet Singh , Nguyen Nam-Trung , Sreej Kamalalayam Rajan
Published: January 2024

Mobility manipulation of liquid droplets is an important task of digital imicrofluidics. Acoustic levitation has revolutionised the contactless manipulation of liquid droplets for various applications. Acoustic levitation technique can be effectively used to manipulate droplets to obtain their coalescence. This paper reports a unique, versatile, and material-independent approach for the vertical coalescence of the droplets suspended in an acoustic levitator. The acoustic power of the levitator is carefully engineered to obtain vertical coalescence of two liquid droplets. Water, 20% and 40% glycerol-water solutions are used as the working liquids. The results of the experiments revealed three outcomes during the coalescence. The outcomes are analysed and discussed.
Solution of Relativistic Feshbach-Villars Spin-1/2 Equations
Authors: Papp Zoltan , Wingard Derek , Vallejo Antonio Garcia
Published: January 2024

We propose method for studying relativistic spin-$1/2$ particles by solving the corresponding Feshbach-Villars equation. We have found that the Feshbach-Villars spin-$1/2$ equations can be formulated as spin-coupled Feshbach-Villars spin-$0$ equations,that results in a Hamiltonian eigenvalue problem.We adopted an integral equation formalism.The potential operators are represented in a discrete Hilbert space basisand the relevant Green's operator has been calculated by a matrix continued fraction.
The RNA helicases DDX19A/B modulate Selinexor sensitivity by regulating MCL1 mRNA nuclear export in leukemia cells
Authors: Maeda Takahiro , Terasaki Tatsuya , Semba Yuichiro , Sasaki Kensuke , Imanaga Hiroshi , Setoguchi Kiyoko , Yamauchi Takuji , Hirabayashi Shigeki , Nakao Fumihiko , Akahane Koshi , Inukai Takeshi , Akashi Koichi
Published: January 2024

Selinexor, a first-in-class exportin1 (XPO1) inhibitor, is an attractive anti-tumor agent because of its unique mechanisms of action; however, its dose-dependent toxicity and lack of biomarkers preclude its wide use in clinical applications. To identify key molecules/pathways regulating Selinexor sensitivity, we performed genome-wide CRISPR/Cas9 dropout screens using two B-ALL lines. We identified, for the first time, that paralogous DDX19A and DDX19B RNA helicases modulate Selinexor sensitivity by regulating MCL1 mRNA nuclear export. While single depletion of either DDX19A or DDX19B barely altered MCL1 protein levels, depletion of both significantly attenuated MCL1 mRNA nuclear export, reducing MCL1 protein levels. Importantly, combining Selinexor treatment with depletion of either DDX19A or DDX19B markedly induced intrinsic apoptosis of leukemia cells, an effect rescued by MCL1 overexpression. Analysis of Depmap datasets indicated that a subset of T-ALL lines expresses minimal DDX19B mRNA levels, and, in fact, either Selinexor treatment or DDX19A depletion effectively induced TP53-independent apoptosis of MOLT-3, a T-ALL line expressing low DDX19B levels. We conclude that XPO1 and DDX19A/B coordinately regulate cellular MCL1 levels and propose that DDX19A/B could serve as biomarkers for Selinexor treatment. Moreover, pharmacological targeting of DDX19 paralogs may represent a potential strategy to induce intrinsic apoptosis in leukemia cells.
Denitrificimonas halotolerans sp. nov., a novel species isolated from landfill leachate
Authors: Wang Shanhui , Yuan Chu , Xu Chengbin , Li Ditian , Zhang Han , Wang Jing , Wang Xin , Li Yan , Jiao Dian , Yuan Siliang , Chen Han , Qiu Dongru
Published: January 2024

A polyphasic taxonomic approach was employed to characterize the strain JX-1T isolated from landfill leachate samples in Wuhan city, Hubei province. Compared to the five most closely related type species, strain JX-1T and Denitrificimonas caeni HY-14T exhibited the highest sequence similarity (96.83%) for 16S rRNA gene, nearly reaching the maximum value of 20.3% for digital DNA-DNA hybridization (DDH), with the highest value of 76.06% for average nucleotide identity (ANI) and the highest value of 78.89% for average amino acid identity (AAI). The genome size of strain JX-1T was 2,778,815 bp with a G + C content of 46.12%. It had a total of 2059 clusters, 2710 proteins, and 498 singletons. The major fatty acids in strain JX-1T were Summed Feature 8 (C18:1ω7c/C18:ω6c)(18.28%), iso-C15:0 (28.52%), and anteiso-C15:0 (13.54%). The respiratory quinones were predominantly Q9 (91.92%) with a minor presence of Q8 (8.08%). The major polar lipids included aminolipid, aminophospholipid, diphosphatidylglycerol, glycolipid, phosphatidylethanolamine, phosphatidylglycerol, and phospholipid. This bacterium showed resilience to elevated salinity and high levels of ammonia nitrogen in physiological experiments. These results of this polyphasic study enabled the genotypic and phenotypic differentiation of the strain analyzed from the closest related species, which confirmed that the strain represented a novel species within the genus Denitrificimonas, for which the name Denitrificimonas halotolerans sp. nov. was proposed with JX-1T (MCCC 1K08958T = CCTCC M2023896T) as the type strain. The strain JX-1T, despite being classified as Denitrificimonas, lacked denitrification-related genes and was solely capable of ammonia assimilation.
Positioning control of robots using a novel nature inspired optimization based neural network
Authors: Kai Guo , Zhi Bo , Sai Wang , Jingjing Ge
Published: January 2024

Positioning control methods for robot arms are offered within a broad framework for the purposes of unification and categorization. In robot control, the issue of Inverse Kinematics (IK) is crucial. Several conventional IK solutions, including geometry, iterations, and algebraic approaches, are insufficient for high-speed solutions and precise positioning. In recent years, the subject of robot IK using neural networks has attracted a great deal of attention, although its precise control is convenient and need improvement. To tackle the IK issue of a UR3 robot, we offer the Global Iterative Sunflower Optimized Binary Multi Layer Perceptron (GISOB-MLP) neural network technique. The GISOB-MLP improves upon previous methods in terms of generalizability, convergence speed, and convergence accuracy. In order to perform medical puncture surgery, which necessitates precise robot positioning to within 1 mm, the study method solves the position error for the UR3 robot IK only with joint angle or less 0.1 degree courses and the output end tool less than 0.1 mm. The study was conducted, and initial results were obtained, with the goal of pinpointing the robot's puncturing application; this laid the groundwork for the robot's eventual widespread use in medical settings.
Nomogram model to predict progression from Preserved Ratio Impaired Spirometry to Chronic Obstructive Pulmonary Disease in inpatient people: Development and validation of a novel model
Authors: Wu jiaxuan , Wang Guoqing , Gan Jiadi , Yang Lan , Zhang Huohuo , Xian Jinghong , Li Yalun , Li Weimin
Published: January 2024

Background
Preserved Ratio Impaired Spirometry (PRISm) is a specific subtype of pre-chronic obstructive pulmonary disease (pre-COPD), defined as FEV1/FVC ≥ 70% and FEV1<80% pred by pulmonary function test (PFT). People with PRISm are at risk of progression to chronic obstructive pulmonary disease (COPD). We developed a model to predict progression in subjects with PRISm.
Methods
We screened out 188 patients whose lung function transitioned from PRISm to COPD, 173 patients with PRISm who underwent at least two lung function tests and remained unchanged for two years in West China hospital. A total of 283 patients were finally included and they were randomly divided into training and validation groups at a 8:2 ratio. Logistic regression was used to create the model, which eventually emerges as a nomogram.
Results
A total of 283 patients were enrolled, 134 patients (47.35%) were eventually diagnosed with COPD. The training cohort included 227 patients and the validation cohort included 56 patients. Through baseline feature comparison and logistic regression, we finally identified seven meaningful variables, including age, body mass index (BMI), FEV1 pred, FEV1/FVC, family history of respiratory disease, respiratory complications and immune related diseases. Accordingly, one nomogram was developed. The areas under the receiver operating characteristic (ROC) curves of this model were 0.89 and 0.86 in the training and validation cohorts, respectively. The model is well calibrated and decision curve analysis (DCA), clinical impact curve (CIC) demonstrated that the predictive model was clinically meaningful.
Conclusion
We developed China’s first prediction model for the progression of lung function from PRISm to COPD in a real-world inpatient population. This model is conducive to early identification of high-risk groups of pulmonary function deterioration, so as to provide timely intervention and delay the occurrence and progression of the disease.
Statistical Investigation of the Standard Height of Occlusal Rim Blocks among Patients Attending Makerere University Dental Hospital, Kampala, Uganda
Authors: Nono David , Mwebesa Ernest , Bagenda Godfrey , Okullo Isaac , Mugisha Rwenyonyi Charles
Published: January 2024

Edentulous patients desire to reinstate oral function and aesthetics in the most comfortable state and to regain their self-esteem. Due to racial anatomic differences, there is a need to ascertain the standard height of Occlusal Rim Blocks (ORBs) for edentulous patients in Uganda. This study was aimed at statistical investigation of the standard height of ORBs among patients attending Makerere University Dental Hospital, Kampala, Uganda. This was a cross-sectional quantitative study that comprised 240 pairs of wax ORBs requested by clinicians for patients in need of complete dentures. Consecutive sampling was used to select the ORBs. The height of the anterior and posterior portions of the maxillary and mandibular ORBs were recorded using a pair of pliers and a flexible ruler, and data were entered into Excel and exported into STATA software version 15 for analysis. The mean height for the anterior portion of maxillary ORBs was 19.4 mm and for the posterior, 17.4 mm after adjustment while the mean height for the mandibular counterparts was 16.4 mm and 16.0 mm, respectively. There was a statistically significant difference between the mean height of ORB before and after adjustments and between the newly established and internationally accepted standard height of ORB. There was no significant sex difference in the mean height of ORBs. Overall, the mean height of ORBs for the Ugandan population was significantly shorter than the internationally accepted standard height. Keywords: Bite registration, Complete denture, Dental hospital, Edentulous patients, Height, Occlusal rim blocks
The Relationship Between the Diversity of Growth Hormone Genes and the Body Weight of Sentul Chickens
Authors: Pratama Shera Aditia , Depison Depison , Gushairiyanto Gushairiyanto
Published: January 2024

This study aims to determine the diversity of growth hormone genes in Sentul chickens using PCR-RFLP. This study used 70 samples of Sentul chicken blood (35 males and 35 females). The research methods are Bodyweight and body weight gain data collection, blood collection, DNA extraction, and PCR-RFLP. Polymorphism analysis included genotype and allele frequencies, Hardy-Weinberg balance, and heterozygosity. The difference in the average body weight against the genotype of the GH|Msp1 gene was tested for the average difference (t-test). The average body weight of DOC - 3 months and body weight gain of DOC - 3 months in male Sentul chickens were significantly different (P <0.05) higher than female Sentul chickens. The GH|MspI gene in Sentul chickens is polymorphic. The Sentul chicken population was in Hardy-Weinberg equilibrium. Heterozygosity in the Sentul chicken population was Ho 0.228, greater than the He value of 0.213. Genotypes (++) and (+-) were significantly different (P<0.05) on body weight and body weight gain of Sentul chickens aged DOC - 3 months. The conclusion of this study, the allele frequency at the GH|MspI Sentul chicken is polymorphic with two genotypes, namely (++) and (+-), and there are two kinds of alleles (+) and (-). Chisquare test results on the GH|mspI Sentul chicken show balance Hardy-Weinberg. Heterozygosity in Sentul chickens showed diversity. The genotype of the GH|Msp1 Sentul chickens with the genotype (++) had higher body weight and body weight gain than those with the (+-) genotype
Identification of Genetic Diversity of P21-Activated Kinases (PAK1) Gene in Senduro Goats and Boerawa Goats using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism)
Authors: Damayanti Heri , Susilorini Tri Eko , Prasetyo Guruh , Hidayah Nur , Efendi Ahmad Zarkasi , Ardiantoro Ari , Septian Wike Andre , Suyadi Suyadi
Published: January 2024

This study aimed to detect genetic diversity associated with litter size traits of the P21-activated kinases (PAK1) gene in Senduro and Boerawa goats using the PCR-RFLP method. The restriction enzyme used is MSP1 (C*CGG). A pair of primers used in this study, namely Forward ((5'-GCTCAGTGTTGTATTAGCAG-3') and Reverse (5'- CAAGATATAAAGCACAGCCG-3') product length 713 bp (access code ENSCHIG00000003071). The samples used in this study consisted of 80 samples of Senduro goats and 20 samples of Boerawa goats from UPT PT-HMT Singosari, Malang Regency. The results showed that the PAK1 exon 9 gene in Senduro goats and Boerawa goats UPT PT-HMT Singosari was uniform (monomorphic), it was because all samples had GG genotype and G allele frequency of 100%. In conclusion, the PAK1 exon 9 / MSP1 gene is monomorphic and does not associate with litter size in Senduro goats and Boerawa goats, so it cannot be used as a genetic marker.
The effect of Moringa oleifera L. leaf extract supplementation on the chemical and sensory quality of beef patties cooked to a well-done level
Authors: Kartikasari Lilik Retna , Hertanto Bayu Setya , Barido Farough Heidar , Swastike Winny , Nuhriawangsa Adi Magna Patriadi
Published: January 2024

This study aimed to evaluate the effect of Moringa oleifera L. leaf extract supplementation on the chemical composition and sensory quality of beef patties cooked to a well-done level. This study used a completely randomized design (CRD) in one direction with five treatments and five replications. The research treatments included the supplementation of Moringa oleifera L. leaf flour extract (MOE) 0% (P0), 1% (P1), 2% (P2), 3% (P3), and 4% (P4). The variables observed included water content, fat, protein, collagen, and sensory attributes. A 15-cm line scale was used to evaluate the intensity of the attributes tested on beef patties by 30 semi-trained panelists. Chemical and organoleptic quality test data were analyzed using Analysis of Variance. If the treatments had a significant effect, then the analysis was continued using the Tukey Test. The results showed that the addition of Moringa leaf flour extract had a very significant effect (P<0.05) on the chemical quality of beef patties (water, fat, and protein content). The use of Moringa oleifera L. leaf extract at the 2% level produces higher protein and fat levels than the use of 4%. Adding MOE up to 4% can increase the intensity of beef patties' color, aroma, and tenderness (P<0.05). The supplementation of Moringa oleifera L. leaf flour extract at the 2% level can produce beef patties with relatively high protein and fat content and can maintain sensory quality.
Successful Pregnancy in Uncorrected Tetralogy of Fallot with Right Aortic Arch: An Interdisciplinary Team Approach
Authors: Iqda Milisani Hayla , Yogibuana Swastika Putri Valerinna
Published: January 2024

Background: Tetralogy of Fallot (ToF) is the most common cyanotic disorder, with a global prevalence of congenital heart disease nearly reaching 10%. The anomalies observed in individuals with ToF result from the anterior and cephalad displacement of the infundibular (outflow tract) component of the interventricular septum. ToF continues to be a significant contributor to maternal morbidity, mortality, and adverse outcomes in newborns with the condition. Case presentation: An 18-year-old woman in her 28th week of pregnancy was referred to our tertiary referral center, Saiful Anwar Hospital, diagnosed with uncorrected Tetralogy of Fallot (TOF), which had been noticed since she was 2 years old. At that time, her parents refused treatment. She became pregnant unexpectedly and was then directed to RSSA for further management. Conclusion: We present a successful case of pregnancy in a woman who has not undergone surgical repair for ToF. An interdisciplinary pregnancy heart team, including a cardiologist, obstetrician, anesthesiologist, midwives, and neonatologists at our tertiary hospital, developed a care plan based on an individualized assessment of the patient's comorbidities and ToF. The life expectancy of this pregnant woman and her baby is higher with a team approach. The choice of contraception and family planning should be part of the discussion and follow-up from the teenage years, allowing patients with ToF to control their fertility and make informed decisions about having children.
Modified Organized Systems by Incorporation of Carbon Allotropes and Derivatives for Electron Shuttle, ET, FRET, MEF, and Quantum Biology Coupling
Authors: García – Quismondo E. , Bracamonte A. Guillermo
Published: January 2024

In this communication, it was afforded in brief to show how high conjugated carbon based structures, and Carbon allotropes could participate as electron shuttle, semiconductors, quantum emitters and Opto-electronic processors within confined Nanostructured Organized systems. In particular was focused on Nanoassemblies such as vesicles, micelles and lipidic Nanoparticles; and incorporating as well insights from other types of Nanomaterials that could afford to develop new organized systems. It should be noted that in these cases the term organized system was used for all types of molecular assembling and Supramolecular systems that formed structures within the Nanoscale. By this manner the incorporation of Opto-electronic active materials permitted to develop very important photo-physical phenomena with high impact perspectives within technology and Life Sciences. Thus, it was leaded to discuss the participation of Carbon based chemical structures incorporated in different confined molecular media to develop i) Electron Transfer (ET) processes; ii) Reaction Electron Transfers (RET); iii) Catalysis; iv) Quantum emissions; v) Fluorescence Resonance Energy Transfer (FRET); vi) non-classical Light; and vii) Nano-Optics. Therefore, it was intended to present the most important physical and chemical phenomena where they could participate as functional high electronic conjugated chemical structures.
Public Opinion About COVID-19 on a Microblog Platform in China: Topic Modeling and Multidimensional Sentiment Analysis of Social Media
Authors: Guo Feipeng , Liu Zixiang , Lu Qibei , Ji Shaobo , Zhang Chen
Published: January 2024

Background

The COVID-19 pandemic raised wide concern from all walks of life globally. Social media platforms became an important channel for information dissemination and an effective medium for public sentiment transmission during the COVID-19 pandemic.

Objective

Mining and analyzing social media text information can not only reflect the changes in public sentiment characteristics during the COVID-19 pandemic but also help the government understand the trends in public opinion and reasonably control public opinion.

Methods

First, this study collected microblog comments related to the COVID-19 pandemic as a data set. Second, sentiment analysis was carried out based on the topic modeling method combining latent Dirichlet allocation (LDA) and Bidirectional Encoder Representations from Transformers (BERT). Finally, a machine learning logistic regression (ML-LR) model combined with a sparse matrix was proposed to explore the evolutionary trend in public opinion on social media and verify the high accuracy of the model.

Results

The experimental results show that, in different stages, the characteristics of public emotion are different, and the overall trend is from negative to positive.

Conclusions

The proposed method can effectively reflect the characteristics of the different times and space of public opinion. The results provide theoretical support and practical reference in response to public health and safety events.
Linking Dementia Pathology and Alteration in Brain Activation to Complex Daily Functional Decline During the Preclinical Dementia Stages: Protocol for a Prospective Observational Cohort Study (Preprint)
Authors: De Sanctis Pierfilippo , Mahoney Jeannette R , Wagner Johanna , Blumen Helena M , Mowrey Wenzhu , Ayers Emmeline , Schneider Claudia , Orellana Natasha , Molholm Sophie , Verghese Joe
Published: January 2024

BACKGROUND

Progressive difficulty in performing everyday functional activities is a key diagnostic feature of dementia syndromes. However, not much is known about the neural signature of functional decline, particularly during the very early stages of dementia. Early intervention before overt impairment is observed offers the best hope of reducing the burdens of Alzheimer disease (AD) and other dementias. However, to justify early intervention, those at risk need to be detected earlier and more accurately. The decline in complex daily function (CdF) such as managing medications has been reported to precede impairment in basic activities of daily living (eg, eating and dressing).

OBJECTIVE

Our goal is to establish the neural signature of decline in CdF during the preclinical dementia period.

METHODS

Gait is central to many CdF and community-based activities. Hence, to elucidate the neural signature of CdF, we validated a novel electroencephalographic approach to measuring gait-related brain activation while participants perform complex gait-based functional tasks. We hypothesize that dementia-related pathology during the preclinical period activates a unique gait-related electroencephalographic (grEEG) pattern that predicts a subsequent decline in CdF.

RESULTS

We provide preliminary findings showing that older adults reporting CdF limitations can be characterized by a unique gait-related neural signature: weaker sensorimotor and stronger motor control activation. This subsample also had smaller brain volume and white matter hyperintensities in regions affected early by dementia and engaged in less physical exercise. We propose a prospective observational cohort study in cognitively unimpaired older adults with and without subclinical AD (plasma amyloid-β) and vascular (white matter hyperintensities) pathologies. We aim to (1) establish the unique grEEG activation as the neural signature and predictor of decline in CdF during the preclinical dementia period; (2) determine associations between dementia-related pathologies and incidence of the neural signature of CdF; and (3) establish associations between a dementia risk factor, physical inactivity, and the neural signature of CdF.

CONCLUSIONS

By establishing the clinical relevance and biological basis of the neural signature of CdF decline, we aim to improve prediction during the preclinical stages of ADs and other dementias. Our approach has important research and translational implications because grEEG protocols are relatively inexpensive and portable, and predicting CdF decline may have real-world benefits.

INTERNATIONAL REGISTERED REPORT

DERR1-10.2196/56726