bluuebunny/crossref_metadata_2025 huggingface dataset:
Imaging the Limbic System in Parkinson’s Disease—A Review of Limbic Pathology and Clinical Symptoms
Authors: Banwinkler Magdalena , Theis Hendrik , Prange Stéphane , van Eimeren Thilo
Published: September 2022

The limbic system describes a complex of brain structures central for memory, learning, as well as goal directed and emotional behavior. In addition to pathological studies, recent findings using in vivo structural and functional imaging of the brain pinpoint the vulnerability of limbic structures to neurodegeneration in Parkinson’s disease (PD) throughout the disease course. Accordingly, dysfunction of the limbic system is critically related to the symptom complex which characterizes PD, including neuropsychiatric, vegetative, and motor symptoms, and their heterogeneity in patients with PD. The aim of this systematic review was to put the spotlight on neuroimaging of the limbic system in PD and to give an overview of the most important structures affected by the disease, their function, disease related alterations, and corresponding clinical manifestations. PubMed was searched in order to identify the most recent studies that investigate the limbic system in PD with the help of neuroimaging methods. First, PD related neuropathological changes and corresponding clinical symptoms of each limbic system region are reviewed, and, finally, a network integration of the limbic system within the complex of PD pathology is discussed.
Effects and Adaptation of Visual-Motor Illusion Using Different Visual Stimuli on Improving Ankle Joint Paralysis of Stroke Survivors—A Randomized Crossover Controlled Trial
Authors: Tanabe Junpei , Amimoto Kazu , Sakai Katsuya , Osaki Shinpei , Yoshihiro Nao , Kataoka Tokuei
Published: September 2022

Visual-motor illusion (VMI) is an intervention to induce kinesthetic sensation from visual stimuli. We aimed to compare the effects of VMI of different visual stimuli on the paralyzed side ankle joint of stroke hemiplegic patients (hemiplegic patients) and to clarify their indication. We applied two types of VMI images of ankle dorsiflexion: ankle dorsiflexion without resistance (standard VMI (S-VMI)) and maximum effort dorsiflexion with resistance (power VMI (P-VMI)). Twenty-two hemiplegic patients were divided into two groups: Group A, which received S-VMI first and P-VMI one week later (n = 11), and Group B, which received P-VMI first and S-VMI one week later (n = 11). Immediate effects were evaluated. Outcomes were the dorsiflexion angle and angular velocity, degree of sense of agency (SoA), and sense of ownership. Patient’s characteristics of cognitive flexibility were assessed using the Trail making test-B (TMT-B). Fugl-Meyer assessment and the Composite-Spasticity-Scale were also assessed. P-VMI was significantly higher than S-VMI in SoA and dorsiflexion angular velocity. Additionally, the degree of improvement in dorsiflexion function with P-VMI was related to TMT-B and degree of muscle tone. Therefore, P-VMI improves ankle function in hemiplegic patients more than S-VMI but should be performed with cognitive flexibility and degree of muscle tone in mind.
Changes in the Epidemiology of Hepatocellular Carcinoma in Asia
Authors: Liu Yao , Liu Lianxin
Published: September 2022

Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide, with high morbidity and mortality, and the incidence is on the rise. HCC imposes a heavy healthcare burden on Asian countries due to the presence of multiple HCC risk factors in this area. Chronic hepatitis B virus (HBV) infection, chronic hepatitis C virus (HCV) infection, non-alcoholic liver disease (NAFLD), aflatoxin and alcohol intake are the causes of HCC that cannot be ignored. Compared with the pre-vaccination era, universal vaccination of newborns reduces the incidence of HCC. Anti-viral therapy with nucleos(t)ide analogues also causes a decline in HCC incidence. Early screening and direct-acting antiviral agent are beneficial to the prevention and treatment of HCV. For HCC caused by NAFLD and other reasons, lifestyle changes are imperative. This paper introduces the epidemiological trends of HCC in Asia and highlight future efforts. Focusing on prevention may be the most effective way to improve the prognosis of this hard-to-treat cancer.
Enhanced Antitumoral Activity of Encapsulated BET Inhibitors When Combined with PARP Inhibitors for the Treatment of Triple-Negative Breast and Ovarian Cancers
Authors: Juan Alberto , Noblejas-López María del Mar , Bravo Iván , Arenas-Moreira María , Blasco-Navarro Cristina , Clemente-Casares Pilar , Lara-Sánchez Agustín , Pandiella Atanasio , Alonso-Moreno Carlos , Ocaña Alberto
Published: September 2022

BRCA1/2 protein-deficient or mutated cancers comprise a group of aggressive malignancies. Although PARPis have shown considerably efficacy in their treatment, the widespread use of these agents in clinical practice is restricted by various factors, including the development of acquired resistance due to the presence of compensatory pathways. BETis can completely disrupt the HR pathway by repressing the expression of BRCA1 and could be aimed at generation combination regimes to overcome PARPi resistance and enhance PARPi efficacy. Due to the poor pharmacokinetic profile and short half-life, the first-in-class BETi JQ1 was loaded into newly developed nanocarrier formulations to improve the effectivity of olaparib for the treatment of BRCAness cancers. First, polylactide polymeric nanoparticles were generated by double emulsion. Moreover, liposomes were prepared by ethanol injection and evaporation solvent method. JQ1-loaded drug delivery systems display optimal hydrodynamic radii between 60 and 120 nm, with a very low polydispersity index (PdI), and encapsulation efficiencies of 92 and 16% for lipid- and polymeric-based formulations, respectively. Formulations show high stability and sustained release. We confirmed that all assayed JQ1 formulations improved antiproliferative activity compared to the free JQ1 in models of ovarian and breast cancers. In addition, synergistic interaction between JQ1 and JQ1-loaded nanocarriers and olaparib evidenced the ability of encapsulated JQ1 to enhance antitumoral activity of PARPis.
TRPM4 Participates in Irradiation-Induced Aortic Valve Remodeling in Mice
Authors: Mpweme Bangando Harlyne , Simard Christophe , Aize Margaux , Lebrun Alexandre , Manrique Alain , Guinamard Romain ,   on behalf of the STOP-AS Investigators
Published: September 2022

Thoracic radiotherapy can lead to cardiac remodeling including valvular stenosis due to fibrosis and calcification. The monovalent non-selective cation channel TRPM4 is known to be involved in calcium handling and to participate in fibroblast transition to myofibroblasts, a phenomenon observed during aortic valve stenosis. The goal of this study was to evaluate if TRPM4 is involved in irradiation-induced aortic valve damage. Four-month-old Trpm4+/+ and Trpm4−/− mice received 10 Gy irradiation at the aortic valve. Cardiac parameters were evaluated by echography until 5 months post-irradiation, then hearts were collected for morphological and histological assessments. At the onset of the protocol, Trpm4+/+ and Trpm4−/− mice exhibited similar maximal aortic valve jet velocity and mean pressure gradient. Five months after irradiation, Trpm4+/+ mice exhibited a significant increase in those parameters, compared to the untreated animals while no variation was detected in Trpm4−/− mice. Morphological analysis revealed that irradiated Trpm4+/+ mice exhibited a 53% significant increase in the aortic valve cusp surface while no significant variation was observed in Trpm4−/− animals. Collagen staining revealed aortic valve fibrosis in irradiated Trpm4+/+ mice but not in irradiated Trpm4−/− animals. It indicates that TRPM4 influences irradiation-induced valvular remodeling.
Imprinted-Zeolite-X-Based Sensor for Non-Enzymatic Detection of Blood Glucose by Potentiometry
Authors: Khasanah Miratul , Widati Alfa Akustia , Handajani Usreg Sri , Mastura Akhsin , Sari Eka Yunicha
Published: September 2022

The development of sensors based on imprinted zeolite X to detect blood glucose through potentiometry was performed. In this study, the sensor was made of a mixture of carbon paste and imprinted zeolite X. Zeolite X was synthesized using a sol–gel-hydrothermal method at a temperature of 100 °C with basic materials of NaAlO2, NaOH, TEOS, and distilled water. The characterization results of XRD showed the presence of specific peaks, which were confirmed with standard zeolite X. Imprinted zeolite X exhibited a 20 times greater adsorption capacity size, and an adsorption efficiency 3 times greater than that of zeolite X. This is thought to be due to the presence of a molecular template within it. The IZ–carbon paste electrode showed optimum performance due to a mass ratio of carbon, paraffin, and imprinted zeolite X of 12:7:1. The electrode performance was expressed by the Nernst factor value of 30 mV/decade, the measuring range of 10−4–10−2 M, the upper detection limit of 1.38 × 10−2 M, and the lower detection limit of 1.28 × 10−4 M, so this electrode can be used for glucose analysis with a normal concentration (70–110 mg/dL or equivalent to 3.8 × 10−3–6.1 × 10−3 M), as well as the glucose concentration of people with diabetes mellitus (>200 mg/dL or about 10−2 M). This electrode showed precision values of 97.14–99.02%, accuracy values of 98.65–99.39%, and electrode response times of 10–13 s. The electrodes showed high stability for more than 5 weeks with 141 uses. The electrodes also showed high selectivity for glucose in the matrix of uric acid, urea, NaCl, and KCl. Therefore, its use as an alternative electrode for routine glucose analysis in the medical field is recommended.
“Metrology” Approach to Data Streams Initiated by Internet Services in the Local Networks
Authors: Filimonova Nina A. , Kolpakov Alexander G. , Rakin Sergei I.
Published: September 2022

The paper presents the results of an experimental investigation and statistical analysis of the data streams generated by popular Internet services. It is found that every investigated Internet service generates data streams of a specific type, possessing specific statistical characteristics. On this basis, it is possible to develop an analog of the classical metrology approach for the data streams generated by Internet services. Furthermore, i the problem of the data streams superposition “at the source” (i.e., on a user’s computer or on a local network) is investigated. It is found that the data streams are additive, with sufficient accuracy for engineering applications (but not exactly additive).
Mesophotic Reefs of the Largest Brazilian Coastal Protected Area: Mapping, Characterization and Biodiversity
Authors: Pereira Pedro H. C. , Lima Gislaine V. , Araujo Julia C. , Gomes Erandy , Côrtes Luís G. F. , Pontes Antonio V. , Recinos Radharanne , Cardoso Andrei , Seoane José C. , Brito Camila C. P.
Published: September 2022

Mesophotic reefs are poorly known worldwide despite their great ecological relevance and management importance for coral reef conservation strategies. To aid in filling this gap, we conducted a pioneering, large-scale survey, covering a total of around 315 miles in length, in the largest Brazilian coastal Marine Protected Area (MPA) Costa dos Corais. From the digital bathymetry model (30 to 50 m depth) generated by a multibeam echo sounder, we selected areas of greater geomorphological diversity for a detailed investigative expedition of mesophotic ecosystems. Various sampling techniques were used: single-beam echo sounders for detailing the relief, a remotely operated underwater vehicle (ROV) for habitat type investigation, baited remote underwater video (BRUV) for collecting images of the fish community, and scuba diving to perform transects describing the benthic and fish community. We analyzed reef environments from 20 to 68 m deep. As a result, we present the mapping and geomorphological characterization of two compartments of mesophotic reefs at 21–45 m depth and an image library of mesophotic ecosystems with the species description and indications of whether it is a new record in the region. Biodiversity data were collected covering eight species of sponges, with greater abundance for Ircinia spp., Aplysina spp., and Xestospongia muta; eight from corals, mainly Siderastrea spp. And Montastrea cavernosa; and 68 species of reef fish, with the Labridae family (including Scarinae—11 species) being the richest. Our results demonstrate the importance of mesophotic reefs for MPA Costa dos Corais reef biodiversity and, with that, the need to protect these areas through the application of local conservation strategies, such as the creation of “no-take zones”.
Bush Encroachment and Large Carnivore Predation Success in African Landscapes: A Review
Authors: Atkinson Holly , Cristescu Bogdan , Marker Laurie , Rooney Nicola
Published: September 2022

Bush encroachment is a habitat change phenomenon that threatens savanna and grassland ecosystems worldwide. In Africa, large carnivores in bush encroached landscapes must adjust to increasing woody plant cover and biomass, which could affect predation success at multiple stages through complex and context-dependent pathways. We highlight, interpret, and compare studies that assessed how bush encroachment or related habitat parameters affect the predation stages of large African carnivores. Bush encroachment may directly or indirectly affect predation success in various ways, including by: (1) altering habitat structure, which may affect hunting efficiency and prey accessibility; (2) changing prey abundance/distribution, with smaller species and browsers being potentially favoured; (3) influencing interference competition within the carnivore guild. For habitat or dietary specialists, and subordinate predators that are vulnerable to both top-down and bottom-up ecosystem effects, these alterations may be detrimental and eventually incur population fitness costs. As the threat of bush encroachment continues, future studies are required to assess indirect effects on competitive interactions within the large African carnivore guild to ensure that conservation efforts are focused. Additionally, to better understand the effects of bush encroachment across Africa, further research is necessary in affected areas as overall little attention has been devoted to the topic.
Distributional Trends and Species Richness of Maryland, USA, Stoneflies (Insecta: Plecoptera), with an Emphasis on the Appalachian Region
Authors: Hogan Phillip N. , Grubbs Scott A.
Published: September 2022

Faunistic studies of regional biodiversity of aquatic insects are increasing in importance as declines are noted globally. Federal and state government conservation attempts for rare and threatened species are predicated upon the initial research of specialized taxonomists and trained field biologists. The reporting of aquatic insect occurrence data provides a baseline for conservation agencies to compare water quality monitoring studies. Updated field work, literature reviews, and database queries for stoneflies from the mid-Atlantic United States of America state of Maryland necessitated an assessment of species diversity for the state. Seven new state records and one new literature record are presented, bringing the total number of species to 122. Chao1 estimates of species richness are presented for diversity hotspots and the state as a whole, indicating that increased sampling is still necessary to fully understand diversity patterns. Accompanying are assessments of elevation trends and adult presence patterns within nine families. Collections are predominantly restricted to the Appalachian region. Herein, we direct future efforts to focus on understudied regions. An outline of distribution knowledge for species is presented to inform upcoming State Wildlife Action Plans.
An Analysis of the Performance of Regional Development Banks (RDB) in Indonesia: Stochastic Frontier Analysis Approach
Authors: Satyagraha Ferdian Timur , Purwono Rudi , Sari Dyah Wulan
Published: September 2022

The dominance of the banking sector shows that the banking industry plays an important role in driving the economy in Indonesia. As part of the national banking industry, Regional Development Banks (RDB) are required to contribute to the economy through optimal performance. One of the important indicators in measuring company performance, including in the banking industry, is the level of efficiency. Efficiency in the banking industry can be observed from a micro and macro perspective, which leads to the ability of banks to survive in conditions of increasingly fierce competition in technology-based products and services, as well as the ability of banks to allocate financial resources to increase investment activities that can stimulate the economy. Therefore, this study examined the level of cost and profit efficiency of the RDB industry in Indonesia for the 2011–2020 period, as well as the internal and external variables that affect RDB inefficiency using Stochastic Frontier Analysis (SFA). The results show that there are no RDB’s efficient in cost and profit. Furthermore, the variables capital adequacy ratio and technology investment impact on increasing cost and profit efficiency, opposite with non-performing loan. Therefore, to optimize the xRDB’s cost and profit efficiency, the main thing that must be done includes managing and improving good quality loans as well as optimizing idle funds.
The Role of Agricultural Biomass as a Renewable Energy Source in European Union Countries
Authors: Janiszewska Dorota , Ossowska Luiza
Published: September 2022

The aim of the research is to assess the potential of agricultural biomass available in European Union countries that can be used for energy purposes. The research took into account the potential of agricultural biomass from: straw from cereal crops, hay from permanent grasslands, natural fertilizers from animal husbandry, cultivation of energy crops on fallow land and waste wood from permanent crops. The study estimated the theoretical potential presenting the energy value of all existing agricultural biomass resources in EU countries and the technical potential taking into account agricultural biomass resources that are not used in agriculture. The research was based on Eurostat data for 2019. The conducted research shows that European Union countries are characterized by a significant potential of agricultural biomass. However, due to the high demand for this resource in agriculture, about 15% of the existing potential can be used for energy purposes. Among the analyzed sources, the highest potential is characterized by straw from cereal crops and by the cultivation of energy crops on fallow land. The conducted research also indicates a large spatial differentiation of the potential of agricultural biomass in European Union countries, which may have a negative impact on the economic efficiency of using this renewable energy source.
COVID-19 Pandemic as a Transitional Event: From the Perspective of the Transition Theory
Authors: Heanoy Eamin Z. , Uzer Tugba , Brown Norman R.
Published: September 2022

The COVID-19 pandemic has affected every aspect of people’s lives across the globe. It is also unique in the way it changed their lives. In this entry, a framework, the Transition Theory, is outlined, which is used to interpret the transitional properties of this pandemic, the ways it differs from other transitional events, and how it impacts the lives and well-being of the individuals. The prediction is that people might consider the pandemic as an important life transition event only if there is a little similarity between their pre-pandemic and post-pandemic lives. Individual differences also need to be considered as those whose lives have been directly affected by the pandemic experience a greater COVID-related change (e.g., job loss vs. no job loss). Lastly, the transitional impact of the pandemic might have a strong link with people’s mental outcomes. These notions call for a longitudinal approach to get an accurate understanding of the pandemic experience while this world-changing event unfolds rather than in retrospect.
Ectomycorrhizal Fungi Modulate Biochemical Response against Powdery Mildew Disease in Quercus robur L.
Authors: Kebert Marko , Kostić Saša , Zlatković Milica , Stojnic Srdjan , Čapelja Eleonora , Zorić Martina , Kiprovski Biljana , Budakov Dragana , Orlović Saša
Published: September 2022

In light of climate change, pedunculate oak (Q. robur L.) was marked as the most threatened European tree species. Pedunculate oak is particularly jeopardized by powdery mildew disease caused by Erysiphe alphitoides. We hypothesized that priming of this tree species with ectomycorrhizal fungi could mitigate biotic stress and produce bioprotective properties against the disease. In this study, we have compared oaks’ foliar physiological and biochemical responses upon infection with E. alphitoides in the presence and absence of ectomycorrhizal fungi (ECM). The main aim of this study was to inspect how ECM modulate an oak’s biochemical response to infection with E. alphitoides, particularly at the level of the accumulation of the main polyamines (putrescine, spermidine, and spermine), soluble osmolytes (proline and glycine betaine), and phenolics (total phenolic content, flavonoids, and condensed tannins). A polyamine quantification was performed after derivatization by using high-performance liquid chromatography (HLPC) coupled with fluorescent detection. Oak seedlings inoculated with ECM fungi exhibited significantly higher levels of putrescine, spermine, and proline compared to non-inoculated seedlings, indicating the priming properties of the ECM. E. alphitoides caused an increase in individual and total polyamine content and lipid peroxidation in oak leaves regardless of the effect of ECM, while causing a decrease in physiological and antioxidative parameters and water use efficiency (WUE). Common biochemical parameters may contribute to understanding the underpinning plant defense mechanisms in three-way interactions among plants and pathogenic and ectomycorrhizal fungi and can be used as reliable adaptability descriptors in the context of climate change.
Comparison of Chemosensory Receptor Genes in the Antennae Transcriptomes of Sirex noctilio and Sirex nitobei (Hymenoptera: Siricidae)
Authors: Wu Weiwei , Hao Enhua , Guo Bing , Yang Huan , Zhou Jingjiang , Ma Mei , Lu Pengfei , Qiao Haili
Published: September 2022

The woodwasp Sirex noctilio Fabricius is a worldwide quarantine pest for forestry that mainly harms conifers, especially Pinus species. Sirex nitobei Matsumura, a native species from China and closely related to S. noctilio. Olfaction and gustation play a vital role in the life movements of woodwasps, but the molecular mechanisms of chemoperception in these species remain unclear. We performed a comparative analysis of 41 odorant receptors (ORs), 13 ionotropic receptors (IRs), and 8 gustatory receptors (GRs) of S. noctilio and 43 ORs, 16 IRs, and 10 GRs of S. nitobei. Phylogenetic analysis showed that two species-specific OR subfamilies were identified in each species. In addition to conserved “antennal IRs”, “divergent IRs”, including 7 SnocIRs and 7 SnitIRs, were found. Moreover, a sugar receptor subfamily (SnocGR5 and SnitGR11), a carbon dioxide receptor subfamily (SnocGR2/GR3 and SnitGR3), and a fructose receptor subfamily (SnitGR9) emerged, but bitter receptors were not identified. The tissue-specific expression profiles showed 36 ORs were enriched in the antennae of S. noctilio. Among them, 19 ORs were female-biased, whereas 4 ORs (SnocOR6/15/18/30) were male-biased. In addition, 34 ORs were highly expressed in S. nitobei antennae, of which 22 ORs were female-biased, whereas SnitOR2/18/30 were male-biased. Seven IRs were enriched in the antennae of both species, of which SnocIR4, 6, 10, 11, and 12 were significantly male-biased, while SnitIR4, 6, 10, 11, and 12 were significantly female-biased. Three GRs were highly expressed in the antennae of both species. SnocGR2 and SnocGR6 were also highly expressed in the head and leg, respectively. In the present study, a total 62 and 69 chemosensory receptor genes were identified in the antennal transcriptomes of S. noctilio and S. nitobei, respectively. Although most receptor genes are homologous, there are also some specific receptor genes, suggesting similarities and differences in molecular mechanisms between the two closely related species. OR genes may be involved in different physiological functions by whether they are expressed in olfactory organs, or obvious gender bias. Our results provide a foundation for further investigating the molecular mechanisms of chemoreception in these two closely related woodwasp species, and establishes a starting point for further research on molecular mechanisms of the olfactory system in symphyta woodwasps.
An Efficient Blockchain Transaction Retrieval System
Authors: Feng Hangwei , Wang Jinlin , Li Yang
Published: September 2022

In the era of the digital economy, blockchain has developed well in various fields, such as finance and digital copyright, due to its unique decentralization and traceability characteristics. However, blockchain gradually exposes the storage problem, and the current blockchain stores the block data in third-party storage systems to reduce the node storage pressure. The new blockchain storage method brings the blockchain transaction retrieval problem. The problem is that when unable to locate the block containing this transaction, the user must fetch the entire blockchain ledger data from the third-party storage system, resulting in huge communication overhead. For this problem, we exploit the semi-structured data in the blockchain and extract the universal blockchain transaction characteristics, such as account address and time. Then we establish a blockchain transaction retrieval system. Responding to the lacking efficient retrieval data structure, we propose a scalable secondary search data structure BB+ tree for account address and introduce the I2B+ tree for time. Finally, we analyze the proposed scheme’s performance through experiments. The experiment results prove that our system is superior to the existing methods in single-feature retrieval, concurrent retrieval, and multi-feature hybrid retrieval. The retrieval time under single feature retrieval is reduced by 40.54%, and the retrieval time is decreased by 43.16% under the multi-feature hybrid retrieval. It has better stability in different block sizes and concurrent retrieval scales.
A Sulfated Abalone Polysaccharide Inhibited SARS-CoV-2 Infection of Vero E6 Cells In Vitro
Authors: Sun Jinghe , Song Shuang , Ai Chunqing , Zhu Beiwei , Yang Jingfeng
Published: September 2022

Sulfate polysaccharides, such as heparin sulfate, have been found to have inhibitory activity against SARS-CoV-2. An abalone polysaccharide, AGSP, was deeply sulfate modified using the chlorosulfonic acid/pyridine method, yielding S-AGSP. AGSP and S-AGSP inhibitions of SARS-CoV-2 infection of Vero E6 cells were tested in vitro. The interference of AGSP or S-AGSP on the binding interaction between the SARS-CoV-2 spike protein and angiotensin-converting enzyme was tested using a biolayer interferometry assay. Results showed that S-AGSP, above a concentration of 1.87 µg/mL, significantly inhibited SARS-CoV-2 infection of Vero E6 cells. Compared with AGSP, S-AGSP obviously weakened the affinity between the SARS-CoV-2 spike protein and ACE2. The polysaccharide’s sulfate content played a vital role in influencing the binding affinity of spike protein to ACE2. Therefore, S-AGSP has potential as a COVID-19 competitive inhibitor as well as a candidate to be repurposed as a prophylactic COVID-19 therapeutic.
Some Results on a New Refinable Class Suitable for Fractional Differential Problems
Authors: Pezza Laura , Tallini Luca
Published: September 2022

In recent years, we found that some multiscale methods applied to fractional differential problems, are easy and efficient to implement, when we use some fractional refinable functions introduced in the literature. In fact, these functions not only generate a multiresolution on R, but also have fractional (non-integer) derivative satisfying a very convenient recursive relation. For this reason, in this paper, we describe this class of refinable functions and focus our attention on their approximating properties.
Vasovagal Syncope Is Associated with Variants in Genes Involved in Neurohumoral Signaling Pathways
Authors: Titov Boris , Matveeva Natalya , Kulakova Olga , Baulina Natalia , Bazyleva Elizaveta , Kheymets Grigory , Rogoza Anatolii , Pevzner Alexander , Favorova Olga
Published: September 2022

Vasovagal syncope (VVS) is the most common cause of sudden loss of consciousness. VVS results from cerebral hypoperfusion, due to abnormal autonomic control of blood circulation, leading to arterial hypotension. It is a complex disease, and its development is largely associated with genetic susceptibility. Since abnormal neurohumoral regulation plays an important role in VVS development, we analyzed the association of VVS with polymorphic variants of ADRA1A, ADRB1, HTR1A, ADORA2A, COMT, and NOS3 genes, the products of which are involved in neurohumoral signaling, in patients with a confirmed VVS diagnosis (157 subjects) and individuals without a history of syncope (161 subjects). We were able to identify the associations between VVS and alleles/genotypes ADRA1A rs1048101, ADRB1 rs1801253, ADORA2A rs5751876, and COMT rs4680, as well as NOS3 rs2070744 in biallelic combination with COMT rs4680. Thus, we are the first to observe, within a single study, the role of the genes that encode α- and β-adrenergic receptors, catechol-O-methyltransferase, adenosine receptors and nitric oxide synthase in VVS development. These findings demonstrate that the genes involved in neurohumoral signaling pathways contribute to the formation of a genetic susceptibility to VVS.
SLC13A5 Deficiency Disorder: From Genetics to Gene Therapy
Authors: Goodspeed Kimberly , Liu Judy S. , Nye Kimberly L. , Prasad Suyash , Sadhu Chanchal , Tavakkoli Fatemeh , Bilder Deborah A. , Minassian Berge A. , Bailey Rachel M.
Published: September 2022

Epileptic encephalopathies may arise from single gene variants. In recent years, next-generation sequencing technologies have enabled an explosion of gene identification in monogenic epilepsies. One such example is the epileptic encephalopathy SLC13A5 deficiency disorder, which is caused by loss of function pathogenic variants to the gene SLC13A5 that results in deficiency of the sodium/citrate cotransporter. Patients typically experience seizure onset within the first week of life and have developmental delay and intellectual disability. Current antiseizure medications may reduce seizure frequency, yet more targeted treatments are needed to address the epileptic and non-epileptic features of SLC13A5 deficiency disorder. Gene therapy may offer hope to these patients and better clinical outcomes than current available treatments. Here, we discuss SLC13A5 genetics, natural history, available treatments, potential outcomes and assessments, and considerations for translational medical research for an AAV9-based gene replacement therapy.
Nonsense-Mediated Decay Targeted RNA (ntRNA): Proposal of a ntRNA–miRNA–lncRNA Triple Regulatory Network Usable as Biomarker of Prognostic Risk in Patients with Kidney Cancer
Authors: Zhou Zhiyue , Hu Fuyan , Huang Dan , Chi Qingjia , Tang Nelson L. S.
Published: September 2022

The most prevalent subtype of renal cell carcinoma (RCC), kidney renal clear cell carcinoma (KIRC) may be associated with a poor prognosis in a high number of cases, with a stage-specific prognostic stratification currently in use. No reliable biomarkers have been utilized so far in clinical practice despite the efforts in biomarker research in the last years. Nonsense-mediated mRNA decay (NMD) is a critical safeguard against erroneous transcripts, particularly mRNA transcripts containing premature termination codons (called nonsense-mediated decay targeted RNA, ntRNA). In this study, we first characterized 296 differentially expressed ntRNAs that were independent of the corresponding gene, 261 differentially expressed miRNAs, and 4653 differentially expressed lncRNAs. Then, we constructed a hub ntRNA–miRNA–lncRNA triple regulatory network associated with the prognosis of KIRC. Moreover, the results of immune infiltration analysis indicated that this network may influence the changes of the tumor immune microenvironment. A prognostic model derived from the genes and immune cells associated with the network was developed to distinguish between high- and low-risk patients, which was a better prognostic than other models, constructed using different biomarkers. Additionally, correlation of methylation and ntRNAs in the network suggested that some ntRNAs were regulated by methylation, which is helpful to further study the causes of abnormal expression of ntRNAs. In conclusion, this study highlighted the possible clinical implications of ntRNA functions in KIRC, proposing potential significant biomarkers that could be utilized to define the prognosis and design personalized treatment plans in kidney cancer management in the next future.
Recommendations for Interpreting and Reporting Silent Carrier and Disease-Modifying Variants in SMA Testing Workflows
Authors: Milligan John N. , Blasco-Pérez Laura , Costa-Roger Mar , Codina-Solà Marta , Tizzano Eduardo F.
Published: September 2022

Genetic testing for SMA diagnosis, newborn screening, and carrier screening has become a significant public health interest worldwide, driven largely by the development of novel and effective molecular therapies for the treatment of spinal muscular atrophy (SMA) and the corresponding updates to testing guidelines. Concurrently, understanding of the underlying genetics of SMA and their correlation with a broad range of phenotypes and risk factors has also advanced, particularly with respect to variants that modulate disease severity or impact residual carrier risks. While testing guidelines are beginning to emphasize the importance of these variants, there are no clear guidelines on how to utilize them in a real-world setting. Given the need for clarity in practice, this review summarizes several clinically relevant variants in the SMN1 and SMN2 genes, including how they inform outcomes for spinal muscular atrophy carrier risk and disease prognosis.
Examining the Relationship between Pes Planus Degree, Balance and Jump Performances in Athletes
Authors: Şahin Fatma Neşe , Ceylan Levent , Küçük Hamza , Ceylan Tülay , Arıkan Gökhan , Yiğit Sevcan , Sarşık Derya Çetin , Güler Özkan
Published: September 2022

The foot absorbs shocks with its arches, muscles, ligaments and joints, and bodyweight transmission and it pushes the body forward during all movement patterns. Pes planus is more important in sports activities that include balance and sports performance, such as walking, running, jumping, or transferring weight to a single lower limb. This study, conducted with this information in mind, aims to examine the effects of pes planus deformity on balance and vertical jump performance. Fifty athletes were included in the study. The presence of pes planus was evaluated according to the Feiss line. Balance measurements were performed with a Balance System SD Biodex. Vertical jump performance was recorded using an Omegawave jumping mat. The relationship between the pes planus grades of the participants and their balance and jump performances was analyzed using the Spearman correlation method. Vertical jump and Limit of stability (LOS) was significantly correlated with pes planus.
Nursing Students and the Human Body: Application of an Ethics Pilot Project
Authors: Garrigues Layla , Soulé Isabelle , Vermeesch Amber
Published: September 2022

This manuscript offers findings from a pilot project which prepares nursing students for embodied professional practice through the lens of ethics. Four undergraduate nursing students were mentored by two nursing faculty in the Dundon-Berchtold Institute Faculty Fellowship Program in the Application of Ethics through an exploration on the ethics of embodiment using an arts pedagogy across one academic year. Inspired by the intersection of nature and health, this project explores the impact of an arts-integrated pedagogy on the human body. The findings from this project provide a natural first step for nursing students to consider multiple interpretations of the human body and to facilitate the students’ development of an embodied ethical practice that is perceptive, empathic, and attuned to themselves as natural beings as well as diverse individuals and populations. The findings from this pilot project presents a pivotal opportunity to guide future nursing curricular development toward holistic, nature-inspired, and mindful-based interventions in order to increase resilience, decrease risk factors of compassion fatigue and burnout, and support nursing students to develop strength-based skills to use in their professional nursing practice.
Research on the Influence of Labor Contract on the Urban Integration of Migrant Workers: Empirical Analysis Based on China’s Micro Data
Authors: Zhao Chuangxin , Tang Manping
Published: September 2022

Using the micro data of the China Labor Dynamics Survey (CLDS), this paper uses factor analysis to construct urban integration indicators and uses the OLS model and intermediary effect model to study the urban integration of 1976 migrant workers in 29 cities in China. This paper empirically analyzes the impact of labor contracts on migrant workers’ urban integration and its mechanism. The study found that: (1) labor contract can significantly promote the urban integration of migrant workers. Further, this conclusion is still tenable after correcting endogenous bias with the 2SLS model and performing a series of robustness tests. (2) Signing labor contracts increases the participation rate of migrant workers in various insurances, enhances the social security level of migrant workers, alleviates the discrimination in the urban labor market, and thus enhances the urban integration of migrant workers. (3) The results of the heterogeneity tests show that the labor contract has a greater impact on the urban integration of the new generation, married and public sector of migrant workers compared with the old generation, unmarried and private sector of migrant workers. Therefore, this paper makes the following suggestions: the government should strengthen labor market supervision, encourage employers to sign long-term labor contracts with migrant workers, improve the social security system for migrant workers, and protect the legitimate rights and interests of migrant workers.
Engagement in Physical Activity Improves after Participation in Pasos Para Prevenir Cancer—An Obesity-Related Cancer Prevention Program in El Paso, Texas
Authors: Valenzuela Roy , Saadiq Stefan , Cobos Sandra , Salinas Jennifer J.
Published: September 2022

Background: This present study experimentally evaluated the Pasos Para Prevenir Cancer (PPPC) program to determine whether participation was associated with improved physical activity engagement. Evidence suggests that obesity prevention programs improve physical activity (PA) engagement and lead to healthier weights, which substantially impacts cancer and cardiometabolic disease risk. There is a shortage of knowledge on the effectiveness of programs tailored to populations along the U.S.–Mexico border. Methods: We collected demographic, nutrition, and physical activity data at baseline, 6 months, and 12 months using the Research Electronic Data Capture (REDCap) for 209 participants. We analyzed the average metabolic equivalents (METS) per week for all physical activity levels and types and the achievement of the recommended METS per week to determine the demographic characteristics most associated with a change between baseline, 6 months, and 12 months. Results: Light activity was the most common activity at all three points, and it slightly increased at 6 months in work settings. Subjects conducted moderate physical activity primarily at home and work, and moderate physical activity increased more compared to vigorous physical activity. Conclusions: Intervention tailoring might improve PA engagement in Mexican Americans residing on the U.S.–Mexico border; however, larger studies that are more diverse are required.
The Relationship between Daily Physical Activity, Psychological Factors, and Vegetative Symptoms in Women with Fibromyalgia: A Cross-Sectional Observational Study
Authors: Navarro-Ledesma Santiago , Pruimboom Leo , Lluch Enrique , Dueñas Lirios , Mena-Del Horno Silvia , Gonzalez-Muñoz Ana
Published: September 2022

Nowadays, there is evidence that relates the amount of physical activity, as well as the impact of psychological factors, to the intensity of symptoms present in patients with fibromyalgia (FM). However, there are no studies which correlate the level of association of physical activity, psychological factors and vegetative symptoms in the FM population. The study has a cross-sectional observational design with 41 participants being recruited from a private clinic and rehabilitation service. The Autonomic Symptom Profile (Compass-31) to assess vegetative symptoms, the GODIN questionnaire to evaluate the level of leisure activity, and the pain catastrophizing scale, Tampa Kinesiophobia Scale and Self-Efficacy Scale to assess psychological factors, were used. A low and significant level of association was found between pain catastrophizing (PCS) and Kinesiophobia (r = 0.398; p < 0.01), as well as with catastrophizing and vegetative symptoms (r = 0.428; p < 0.05). Furthermore, a low and significant level of association was also found between self-efficacy and vegetative symptoms (r = 0.397; p < 0.05). No association was found between the level of daily physical activity (measured by the Godin Leisure questionnaire) and vegetative symptoms, nor with any psychological factor studied. There is an association between vegetative symptoms and psychological factors. Nevertheless, more research which takes other factors into account, such as lifestyle and nutritional, is needed.
Weekly Training Load across a Standard Microcycle in a Sub-Elite Youth Football Academy: A Comparison between Starters and Non-Starters
Authors: Teixeira José E. , Branquinho Luís , Ferraz Ricardo , Leal Miguel , Silva António J. , Barbosa Tiago M. , Monteiro António M. , Forte Pedro
Published: September 2022

Compensatory training sessions have been highlighted as useful strategies to solve the differential weekly training load between the players’ starting status. However, the influence of the players’ starting status is still understudied in sub-elite youth football. Thus, the aim of this study was to compare the weekly training load on a standard microcycle in starters and non-starters of a sub-elite youth football academy. The weekly training load of 60 young sub-elite football players was monitored during a 6-week period using an 18 Hz global positioning system (GPS), 1 Hz telemetry heart rate, rating of perceived exertion (RPE), and total quality recovery (TQR). The total distance (TD) covered presented a significant difference between starters and non-starters with a moderate effect (t = −2.38, Δ = −428.03 m, p = 0.018, d = 0.26). Training volume was higher in non-starters than in starter players (TDStarters = 5105.53 ± 1684.22 vs. TDNon-starters = 5533.56 ± 1549.26 m). Significant interactive effects were found between a player’s starting status, playing time, and session duration in overall training load variables for within (F = 140.46; η2 = 0.85; p < 0.001) and between-subjects (F = 11.63 to 160.70; η2 = 0.05 to 0.76; p < 0.001). The player’s starting status seems to only influence the training volume in sub-elite youth football, unless one considers the covariance of the playing time and session duration. Consequently, coaches should prioritize complementary training to equalize training volume and emphasize similar practice opportunities for non-starters. Future studies should evaluate the gap between training and match load, measuring the impact of recovery and compensatory sessions.
Community-Dwelling Older Adults’ Intended Use of Different Types of Long-Term Care in China and Its Associated Factors Based on the Andersen Behavioral Model
Authors: Che Run-Ping , Cheung Mei-Chun
Published: September 2022

In light of the increased demand for long-term care services in China, there is an ongoing discussion on what factors contribute to older adults’ intended use of long-term care services. This study empirically recruited 239 community-dwelling older adults aged ≥60 years in China and explored factors influencing their intended use of four types of long-term care (basic life care, basic medical care, rehabilitation care, and psychological care) based on the Andersen behavioral model (i.e., predisposing characteristics, enabling resources, and need factors). The results showed that older adults were most likely to use psychological care. Age (as the predisposing characteristic) was the significant predictor of the intended use of four types of care. Regarding the intended use of basic life care, the enabling resources of marital status, household composition, income, as well as need factors of preference for the care setting, were influential. Moreover, income and need factors of self-rated physical health status were only two variables associated with the intended use of basic medical care. Concerning the intended use of rehabilitation care, household composition, income, self-rated physical health status, and preference for the care setting were significant predictors. The intended use of psychological care was influenced by enabling resources of marital status, household composition, and need factors of self-rated physical health status, preference for the care setting, and preference for the caregiver. These results can promote the sensitivity of policymakers and caregivers to the community-dwelling older adults’ intended use of long-term care and contribute to the delivery of appropriate care services by public policy.
SARS-CoV-2 Seroprevalence in Those Utilizing Public Transportation or Working in the Transportation Industry: A Rapid Review
Authors: Heiskanen Aliisa , Galipeau Yannick , Langlois Marc-André , Little Julian , Cooper Curtis L.
Published: September 2022

Proximity and duration of social contact while working or using public transportation may increase users’ risk of SARS-CoV-2 exposure. This review aims to assess evidence of an association between use of public transportation or work in the transportation industry and prevalence of SARS-CoV-2 antibodies as well as to identify factors associated with seropositivity in transit users. A literature search of major databases was conducted from December 2019 to January 2022 using key worlds including “seroprevalence”, “SARS-CoV-2”, and “public transit”. A narrative review of included studies was completed for the following categories: those working in the transportation industry, healthcare workers relying on public transit, and population-based studies. The association between work in the transit industry and seroprevalence varied based on location, demographic characteristics, and test sensitivities. No association was found in healthcare workers. Several population-based studies indicated higher seroprevalence in those using public transit. Overall seroprevalence estimates varied based on geographic location, population demographics, study methodologies, and calendar date of assessment. However, seropositivity was consistently higher in racial minorities and low-income communities.