bluuebunny/crossref_metadata_2025 huggingface dataset:
A Quantile Regression Random Forest-Based Short-Term Load Probabilistic Forecasting Method
Authors: Dang Sanlei , Peng Long , Zhao Jingming , Li Jiajie , Kong Zhengmin
Published: January 2022

In this paper, a novel short-term load forecasting method amalgamated with quantile regression random forest is proposed. Comprised with point forecasting, it is capable of quantifying the uncertainty of power load. Firstly, a bespoke 2D data preprocessing taking advantage of empirical mode decomposition (EMD) is presented. It can effectively assist subsequent point forecasting models to extract spatial features hidden in the 2D load matrix. Secondly, by exploiting multimodal deep neural networks (DNN), three short-term load point forecasting models are conceived. Furthermore, a tailor-made multimodal spatial–temporal feature extraction is proposed, which integrates spatial features, time information, load, and electricity price to obtain more covert features. Thirdly, relying on quantile regression random forest, the probabilistic forecasting method is proposed, which exploits the results from the above three short-term load point forecasting models. Lastly, the experimental results demonstrate that the proposed method outperforms its conventional counterparts.
Structural Model of Power Grid Stabilization in the Green Hydrogen Supply Chain System—Conceptual Assumptions
Authors: Frankowska Marzena , Mańkowska Marta , Rabe Marcin , Rzeczycki Andrzej , Szaruga Elżbieta
Published: January 2022

The paper presents the conceptual assumptions of research concerning the design of a theoretical multi-criteria model of a system architecture to stabilize the operation of power distribution networks based on a hydrogen energy buffer, taking into account the utility application of hydrogen. The basis of the research process was a systematic literature review using the technique of in-depth analysis of full-text articles and expert consultations. The structural model concept was described in two dimensions in which the identified variables were embedded. The first dimension includes the supply chain phases: procurement and production with warehousing and distribution. The second dimension takes into account a comprehensive and interdisciplinary approach and includes the following factors: technical, economic–logistical, locational, and formal–legal.
Multi-Objective Approach for Managing Uncertain Delivery from Renewable Energy Sources within a Peer-to-Peer Energy Balancing Architecture
Authors: Drabecki Mariusz , Toczyłowski Eugeniusz
Published: January 2022

On the energy markets, conscious customers may exist who are not only interested in minimising the cost of energy purchase, but, simultaneously, in optimising some other quality criteria (arising from ecological concerns, or social responsibility of the energy producers). In this paper, we develop both a mathematical optimisation problem and a market framework for balancing a power system in a peer-to-peer market setup, where product differentiation can be considered directly on the market. Thus, origins of energy may be clearly identified, and product quality characteristics can be understood by various actors (including households). We derive a multi-objective (mixed-integer) linear programming optimisation problem for balancing the energy system in a peer-to-peer energy trading environment, where not only the cost but also other additional quality criteria are considered. We have identified many possible actors to be present within the proposed market setup. They include consumers, producers, brokers and flexible prosumers with storage. The approach was tested on the IEEE 30-bus standard test system, over three different scenarios, by analysing the impact of various actors/peers activities and different extensions. It has been shown that a multi-objective energy balancing scheme may be developed through crafted optimisation problem and that each type of studied peers may bring some added value to the power system balancing.
Development of Bus and Line Control Method for Short-Circuit Current Reduction Using Genetic Programming
Authors: Son Dabin , Han Sangwook
Published: January 2022

In this study, genetic programming (GP) is used for optimizing bus and line separation methods to reduce the short-circuit current. Expanding power systems led to intensive electric power and more transmission lines, reducing the grid impedance. This increases the short-circuit currents that occur during failure, and it is impossible to continue developing higher-capacity breakers to accommodate such short-circuit currents. Therefore, the short-circuit currents must be managed systematically through busbar separation and line separation. However, there are countless possible bus and line separation schemes for power systems. Furthermore, to comply with power-system reliability standards, no lines or transformers should be overloaded after such controls are applied. This paper proposes the use of GP to optimize the bus and line separation methods for obtaining a solution. The solutions are limited to methods that can be implemented in real power systems, reducing the convergence probability and optimization time. The proposed technique is useful for designing power systems with consideration of the short-circuit current.
Partial Y-Bus Factorization Algorithm for Power System Dynamic Equivalents
Authors: Kim Soobae , Overbye Thomas J.
Published: January 2022

This paper presents a partial Y-bus factorization algorithm to reduce the size of a power system model for transient stability analysis. In the proposed approach, steady-state operating conditions for dynamic equivalents are maintained using the traditional Ward admittance method. Fictitious generators are attached at boundary buses to preserve transient behavior following a disturbance. The equivalent dynamic effects from eliminated generators can be maintained by choosing appropriate dynamic parameters of fictitious generators, including machine inertia, transient reactance, and the damping coefficient. Parameters are determined using the idea that the contributions from external generators mostly depend on the network configuration and impedance characterized by the Y-bus matrix. The fictitious generators’ dynamic parameters are determined by conducting partial Y-bus factorization on dynamic parameter matrices. The proposed method’s performance is validated by conducting case studies with the IEEE 118-bus system and a 10,000 synthetic western U.S. power grid model and comparing simulation outcomes between the full system and reduced equivalent models. Simulation comparisons show that the equivalent model maintains high accuracy. The proposed method is promising alternative solution for power system dynamic equivalents.
Methodology for Defining Priority Locations for Carrying out a Forest Inventory in Points with Conflict between Urban Tree Planting and the Electricity Grid
Authors: Sant Ana William de Oliveira , Faria Jefferson de , Zavarize Mauro dos Santos , Pezente Daniel Pazini , Barbosa Vanessa de Castro , Spacek Anderson Diogo , Vianna Marcelo Pinto , Junior Oswaldo Hideo Ando
Published: January 2022

The Association of the Coal Industry of Santa Catarina (SATC), together with the State Electric Energy Company (CEEE/Equatorial) of the Brazilian state of Rio Grande do Sul (RS), seeks solutions to reduce conflicts between vegetation and electrical networks. This study was carried out having as an area for pilot the city of Porto Alegre, RS, where the distributor is responsible for the supply of electricity. The objective of this proposal was to define locations for carrying out a vegetation inventory through a predictive model. From satellite images, the existing vegetation in the distribution network was digitally extracted and, from this, five prioritization factors were associated via Hierarchical Process Analysis (HPA), resulting in an equation with consistency ratio where each factor is given a specific weight, resulting in a priority map. The final model is explained by 36% by the number of power outages, 21.5% by vegetation close to the grid, 21% by the affected population, 16.9% by the type of consumer, and 4.6% by the area from the neighborhood. This combination of factors resulted in ‘red zones’ in the pilot area, of which 100 points were chosen for carrying out a forest inventory, with an expected sampling significance of at least 97%. This makes it possible to develop a more assertive and spatially oriented forest inventory, and this model can be replicated in different urban centers.
Influence of the Car Movable Aerodynamic Elements on Fast Road Car Cornering
Authors: Piechna Janusz Ryszard , Kurec Krzysztof , Broniszewski Jakub , Remer Michał , Piechna Adam , Kamieniecki Konrad , Bibik Przemysław
Published: January 2022

In the case of road cars, road safety is the primary factor. The geometry of high-speed road cars has no regulatory restrictions. In addition to the high engine power and effective shape, they can use various types of additional movable aerodynamic elements to adjust their aerodynamic characteristics to the road conditions. Based on the geometry of a two-seater prototype of such a vehicle, a numerical analysis of the influence of a number of additional movable aerodynamic elements on its aerodynamic characteristics was performed. Several of them were installed on the prototype. An electronic system recording a number of motion parameters of the entire car body and some of its movable elements installed on the body was designed and built. The system has been adapted to program the motion of additional aerodynamic elements according to the set algorithms of their activation, temporarily changing the aerodynamic characteristics of the car. An experimental study of the effect of changes in the aerodynamic characteristics of the prototype on its dynamic properties during a drive through a test road section was carried out. It was shown to what extent an average driver can increase the safe speed of the curve of the road using the possibilities of moving aerodynamic elements installed on it.
Approaching Quietness as an Urban Sustainability Opportunity
Authors: Tsaligopoulos Aggelos , Matsinos Yiannis G.
Published: January 2022

Quietness in an urban environment is vital for the well-being of city residents. Nevertheless, the ambiguity in the conceptualization of the terms noise and quietness as urban acoustic planning and design objectives, has resulted in two different approaches: the soundscape approach and the noise control approach. The main purpose of this research is to supplement the existing approaches by proposing a new ecological acoustics approach in order to identify quiet areas in the city of Mytilene (Lesbos Island, North Aegean, Greece). The use of the soundscape approach involved the participation of Mytilene’s residents and the collection of subjective and objective eligibility criteria. By means of Multi-Criteria Decision Making two urban green areas were highlighted as potential quiet areas. For the noise control approach, road noise maps have been created through a commercial noise mapping software, validated by trough measurements. As a result, two areas located in the outskirts of the city were highlighted. Finally, the novel ecological acoustics approach involved acoustic recordings and the extraction of the Composite Urban Quietness Index (CUQI). The outcome of this approach converged with the soundscape approach results. Quietness, as an urban acoustic planning and design goal, could be viewed as an opportunity for ecologically sustainable urban environments.
The Genetic Differences and Structure of Selected Important Populations of the Endangered Taxus baccata in the Czech Republic
Authors: Komárková Martina , Novotný Petr , Cvrčková Helena , Máchová Pavlína
Published: January 2022

Taxus baccata L. (common yew) is an endangered tree species in the Czech Republic. However, its natural occurrence has not been adequately protected in all areas of the country. The aim of this study is to determine whether the yew population in the newly established Mařeničky seed orchard (TS_L) enables mixing with other Czech yew populations. Using a set of nuclear microsatellites, the genetic diversity in the Lužické Mountains (TS_L) and in selected wild-provenance populations from the Czech Republic (Jílovské yews, TS_J; Březinské yews, TS_B, and yews from Moravský Karst, TS_M) was studied, as they could be donor sources for potential translocation activities. We observed that the level of genetic diversity within the four Czech yew units that were investigated was high. An analysis of the molecular variance (AMOVA) showed 7% variation among populations, and the genetic differentiation values were low to moderate (FST = 0.042–0.108). According to a STRUCTURE analysis, high genetic similarity was observed between the TS_L and TS_B units. Our results provide important genetic suggestions on how conservation management can be designed to maximize its success.
Characteristics and Anaerobic Co-Digestion of Press Water from Wood Fuel Preparation and Digested Sewage Sludge
Authors: Sailer Gregor , Empl Florian , Kuptz Daniel , Silberhorn Martin , Ludewig Darwin , Lesche Simon , Pelz Stefan , Müller Joachim
Published: January 2022

Technical drying of harvested wood fuels is heat and energy consuming, while natural pre-drying in the forest, e.g., in stacks or storage piles, is accompanied by energy losses through natural degradation processes. Dewatering of energy wood by mechanical pressing is an innovative method to reduce the moisture content prior to thermal drying while producing press waters (PW, also referred to as wood juice) as a by-product. To date, the characteristics and utilization potentials of PW are largely unknown. In this study, three different spruce- and poplar-based PW were analyzed for their characteristics such as dry matter (DM), organic dry matter (oDM) concentration, pH-value, element concentration or chemical compounds. Additionally, they were used for anaerobic digestion (AD) experiments with digested sewage sludge (DSS) serving as inoculum. The fresh matter-based DM concentrations of the PW were between 0.4 and 3.2%, while oDM concentrations were between 87 and 89%DM. The spruce-based PW were characterized by lower pH-values of approx. 4.4, while the poplar-based PW was measured at pH 8. In the AD experiments, DSS alone (blank variant) achieved a specific methane yield of 95 ± 26 mL/goDM, while the mixture of spruce-based PW and DSS achieved up to 160 ± 12 mL/goDM, respectively. With further research, PW from wood fuel preparation offer the potential to be a suitable co-substrate or supplement for AD processes.
Byproducts (Flour, Meals, and Groats) from the Vegetable Oil Industry as a Potential Source of Antioxidants
Authors: Multescu Mihaela , Marinas Ioana Cristina , Susman Iulia Elena , Belc Nastasia
Published: January 2022

The present study presents the use of photochemiluminescence assay (PCL) and 2,2 diphenyl-1-picryl-hydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), the ferric reducing antioxidant power (FRAP), and cupric ion reducing antioxidant capacity (CUPRAC) methods for the measurement of lipid-soluble antioxidant capacity (ACL) of 14 different byproducts obtained from the vegetable oil industry (flour, meals, and groats). The research showed that the analyzed samples contain significant amounts of phenolic compounds between 1.54 and 74.85 mg gallic acid per gram of byproduct. Grape seed flour extract had the highest content of total phenolic compounds, 74.85 mg GAE/g, while the lowest level was obtained for the sunflower groats, 1.54 mg GAE/g. DPPH values varied between 7.58 and 7182.53 mg Trolox/g of byproduct, and the highest antioxidant capacity corresponded to the grape seed flour (7182.53 mg Trolox/g), followed by walnut flour (1257.49 mg Trolox/g) and rapeseed meals (647.29 mg Trolox/g). Values of ABTS assay of analyzed samples were between 0 and 3500.52 mg Trolox/g of byproduct. Grape seed flour had the highest value of ABTS (3500.52 mg Trolox/g), followed by walnut flower (1423.98) and sea buckthorn flour (419.46). The highest values for FRAP method were represented by grape seed flour (4716.75 mg Trolox/g), followed by sunflower meals (1350.86 mg Trolox/g) and rapeseed flour (1034.92 mg Trolox/g). For CUPRAC assay, grape seed flour (5936.76 mg Trolox/g) and walnut flour (1202.75 mg Trolox/g) showed the highest antioxidant activity. To assess which method of determining antioxidant activity is most appropriate for the byproducts analyzed, relative antioxidant capacity index (RACI) was calculated. Depending on the RACI value of the analyzed byproducts, the rank of antioxidant capacity ranged from −209.46 (walnut flour) to 184.20 (grape seed flour). The most sensitive methods in developing RACI were FRAP (r = 0.5795) and DPPH (r = 0.5766), followed by CUPRAC (r = 0.5578) and ABTS (r = 0.4449), respectively. Strong positive correlations between the antioxidant capacity of lipid-soluble compounds measured by PCL and other methods used for determining antioxidant activity were found (r > 0.9). Analyses have shown that the different types of byproducts obtained from the vegetable oil industry have a high antioxidant activity rich in phenolic compounds, and thus their use in bakery products can improve their nutritional quality.
The Interactive Effect of Genetic and Epigenetic Variations in FKBP5 and ApoE Genes on Anxiety and Brain EEG Parameters
Authors: Kuznetsova Irina L. , Ponomareva Natalya V. , Alemastseva Ekaterina A. , Manakhov Andrey D. , Andreeva Tatyana V. , Gusev Fedor E. , Rogaev Evgeny I.
Published: January 2022

FKBP51 is a key stress-responsive regulator of the hypothalamic–pituitary–adrenal axis. To elucidate the contribution of rs1360780 FKBP5 C/T alleles to aging and longevity, we genotyped FKBP5 in a cohort of 800 non-demented and Alzheimer’s disease (AD) subjects of different age, taking into account the allele state of ApoE ε4, the major risk factor for AD. Furthermore, we searched for the association of FKBP5 with subcohorts of non-demented subjects evaluated for anxiety and resting-state quantitative EEG characteristics, associated with cognitive, emotional, and functional brain activities. We observed that increased state anxiety scores depend on the combination of the FKBP5 and ApoE genotypes and on the DNA methylation state of the FKBP5 promoter and ApoE genotype. We also found a significant gender-dependent correlation between FKBP5 promoter methylation and alpha-, delta-, and theta-rhythms. Analysis of the FKBP5 expression in an independent cohort revealed a significant upregulation of FKBP5 in females versus males. Our data suggest a synergistic effect of the stress-associated (FKBP5) and neurodegeneration-associated (ApoE) gene alleles on anxiety and the gender-dependent effect of FKBP5 on neurophysiological brain activity.
Stalling of Eukaryotic Translesion DNA Polymerases at DNA-Protein Cross-Links
Authors: Yudkina Anna V. , Shilkin Evgeniy S. , Makarova Alena V. , Zharkov Dmitry O.
Published: January 2022

DNA-protein cross-links (DPCs) are extremely bulky adducts that interfere with replication. In human cells, they are processed by SPRTN, a protease activated by DNA polymerases stuck at DPCs. We have recently proposed the mechanism of the interaction of DNA polymerases with DPCs, involving a clash of protein surfaces followed by the distortion of the cross-linked protein. Here, we used a model DPC, located in the single-stranded template, the template strand of double-stranded DNA, or the displaced strand, to study the eukaryotic translesion DNA polymerases ζ (POLζ), ι (POLι) and η (POLη). POLι demonstrated poor synthesis on the DPC-containing substrates. POLζ and POLη paused at sites dictated by the footprints of the polymerase and the cross-linked protein. Beyond that, POLζ was able to elongate the primer to the cross-link site when a DPC was in the template. Surprisingly, POLη was not only able to reach the cross-link site but also incorporated 1–2 nucleotides past it, which makes POLη the most efficient DNA polymerase on DPC-containing substrates. However, a DPC in the displaced strand was an insurmountable obstacle for all polymerases, which stalled several nucleotides before the cross-link site. Overall, the behavior of translesion polymerases agrees with the model of protein clash and distortion described above.
Investigating the Relationships between COVID-19 Quality of Life, Loneliness, Happiness, and Internet Addiction among K-12 Teachers and School Administrators—A Structural Equation Modeling Approach
Authors: Karakose Turgut , Ozdemir Tuncay Yavuz , Papadakis Stamatios , Yirci Ramazan , Ozkayran Secil Eda , Polat Hakan
Published: January 2022

It is well acknowledged that the roles of both school administrators and teachers have changed due to the global education crisis caused by COVID-19. During this challenging and critical period, it is essential to investigate how those working in the education sector who undertake strategic tasks for sustainable education are affected by the new conditions brought about by the COVID-19 pandemic. This study investigates the interrelationships between COVID-19 quality of life, loneliness, happiness, and Internet addiction. The research was designed according to the relational survey model, was conducted with 432 school administrators and teachers working in K-12 schools. The research data was collected through online questionnaires, and structural equation modelling (SEM) was used to test and analyze proposed hypotheses. The study’s results revealed a positive relationship between the COVID-19 related quality of life and loneliness, and that loneliness significantly positively predicts Internet addiction. In this context, due to the impact of COVID-19 on the life quality, the participants’ loneliness levels significantly increased, and this increase in loneliness caused them to become addicted to using the Internet. Interestingly, it was also determined that a positive relationship exists between loneliness and happiness and that as the loneliness of individuals increased, their level of happiness also increased. In many studies conducted prior to the COVID-19 pandemic, a negative relationship was revealed between loneliness and happiness. In the current study conducted during the pandemic, the relationship between the two variables was positive. SEM results revealed that COVID-19 directly affects the quality of life, Internet addiction, loneliness, and happiness of school administrators and teachers. Furthermore, it was determined that Internet addiction indirectly affects the relationship between loneliness and happiness.
Exosome-Based Treatment for Atherosclerosis
Authors: Heo Jeongyeon , Kang Hara
Published: January 2022

Atherosclerosis is an inflammatory disease in which lipids accumulate on the walls of blood vessels, thickening and clogging these vessels. It is well known that cell-to-cell communication is involved in the pathogenesis of atherosclerosis. Exosomes are extracellular vesicles that deliver various substances (e.g., RNA, DNA, and proteins) from the donor cell to the recipient cell and that play an important role in intercellular communication. Atherosclerosis can be either induced or inhibited through cell-to-cell communication using exosomes. An understanding of the function of exosomes as therapeutic tools and in the pathogenesis of atherosclerosis is necessary to develop new atherosclerosis therapies. In this review, we summarize the studies on the regulation of atherosclerosis through exosomes derived from multiple cells as well as research on exosome-based atherosclerosis treatment.
Characteristics of an In Vitro Mesenteric Lymph Node Cell Suspension Model and Its Possible Association with In Vivo Functional Evaluation
Authors: Feng Saisai , Li Jing , Qu Dingwu , Tian Fengwei , Yu Leilei , Zhang Hao , Chen Wei , Zhao Jianxin , Zhai Qixiao
Published: January 2022

In a previous study, we uncovered three immune-responsive patterns of gut microbes using an in vitro mesenteric lymph node cell suspension model, abbreviated as the MLN model hereafter. We used Akkermansia muciniphila and Clostridium butyricum as the first group directly inducing an immune response, Bifidobacterium sp. and Bacteroides sp. as the second group evoking an immune response with the help of stimuli (anti-CD3 and anti-CD28 antibodies), and Lactobacillus sp. as the third group blunting the immune response with or without stimuli. Our group previously clarified the immune-activation characteristics of A. muciniphila and linked its in vivo immune induction effect in GF and SPF mice under homeostasis. In the present study, we supplemented the characteristics of C. butyricum and B. bifidum in the in vitro MLN model and addressed the specific elements of the model. Finally, we used an in vivo TNBS-challenge model to show the functional differences between these species with different response patterns in vitro. The results showed that C. butyricum and B. bifidum evoked an immune response in vitro in a dose-dependent and strain-unique manner. Although TLR2, rather than TLR4, is indispensable for immune activation in the present in vitro model, it may not involve interaction between TLR2 and bacterial ligands. Like the PBMC model, the present in vitro MLN model is highly dependent on cell resources and should be given more attention when used to conduct a quantitative comparison. Finally, a mixture of two strong immunogenic strains, A. muciniphila and C. butyricum, significantly increased the mortality of TNBS-challenged (2,4,6-trinitrobenzene sulfonic acid, TNBS) mice, indicating a possible link between the in vitro MLN model and in vivo functional evaluation. However, more evidence is needed to clarify the associations and underlying mechanisms.
The Role of Vitamin A in Retinal Diseases
Authors: Sajovic Jana , Meglič Andrej , Glavač Damjan , Markelj Špela , Hawlina Marko , Fakin Ana
Published: January 2022

Vitamin A is an essential fat-soluble vitamin that occurs in various chemical forms. It is essential for several physiological processes. Either hyper- or hypovitaminosis can be harmful. One of the most important vitamin A functions is its involvement in visual phototransduction, where it serves as the crucial part of photopigment, the first molecule in the process of transforming photons of light into electrical signals. In this process, large quantities of vitamin A in the form of 11-cis-retinal are being isomerized to all-trans-retinal and then quickly recycled back to 11-cis-retinal. Complex machinery of transporters and enzymes is involved in this process (i.e., the visual cycle). Any fault in the machinery may not only reduce the efficiency of visual detection but also cause the accumulation of toxic chemicals in the retina. This review provides a comprehensive overview of diseases that are directly or indirectly connected with vitamin A pathways in the retina. It includes the pathophysiological background and clinical presentation of each disease and summarizes the already existing therapeutic and prospective interventions.
Mesothelin Expression Is Not Associated with the Presence of Cancer Stem Cell Markers SOX2 and ALDH1 in Ovarian Cancer
Authors: Nunes Mariana , Pacheco Francisca , Coelho Ricardo , Leitão Dina , Ricardo Sara , David Leonor
Published: January 2022

Mesothelin (MSLN) overexpression (OE) is a frequent finding in ovarian carcinomas and increases cell survival and tumor aggressiveness. Since cancer stem cells (CSCs) contribute to pathogenesis, chemoresistance and malignant behavior in ovarian cancer (OC), we hypothesized that MSLN expression could be creating a favorable environment that nurtures CSCs. In this study, we analyzed the expression of MSLN and CSC markers SOX2 and ALDH1 by immunohistochemistry (IHC) in different model systems: primary high-grade serous carcinomas (HGSCs) and OC cell lines, including cell lines that were genetically engineered for MSLN expression by either CRISPR-Cas9-mediated knockout (Δ) or lentivirus-mediated OE. Cell lines, wild type and genetically engineered, were evaluated in 2D and 3D culture conditions and xenografted in nude mice. We observed that MSLN was widely expressed in HGSC, and restricted expression was observed in OC cell lines. In contrast, SOX2 and ALDH1 expression was limited in all tissue and cell models. Most importantly, the expression of CSC markers was independent of MSLN expression, and manipulation of MSLN expression did not affect CSC markers. In conclusion, MSLN expression is not involved in driving the CSC phenotype.
Structure, Function and Regulation of the Plasma Membrane Calcium Pump in Health and Disease
Authors: Krebs Joachim
Published: January 2022

In this review, I summarize the present knowledge of the structural and functional properties of the mammalian plasma membrane calcium pump (PMCA). It is outlined how the cellular expression of the different spliced isoforms of the four genes are regulated under normal and pathological conditions.
Liposomal Formulation of a PLA2-Sensitive Phospholipid–Allocolchicinoid Conjugate: Stability and Activity Studies In Vitro
Authors: Kobanenko Maria K. , Tretiakova Daria S. , Shchegravina Ekaterina S. , Antipova Nadezhda V. , Boldyrev Ivan A. , Fedorov Alexey Yu. , Vodovozova Elena L. , Onishchenko Natalia R.
Published: January 2022

To assess the stability and efficiency of liposomes carrying a phospholipase A2-sensitive phospholipid-allocolchicinoid conjugate (aC-PC) in the bilayer, egg phosphatidylcholine and 1-palmitoyl-2-oleoylphosphatidylglycerol-based formulations were tested in plasma protein binding, tubulin polymerization inhibition, and cytotoxicity assays. Liposomes L-aC-PC10 containing 10 mol. % aC-PC in the bilayer bound less plasma proteins and were more stable in 50% plasma within 4 h incubation, according to calcein release and FRET-based assays. Liposomes with 25 mol. % of the prodrug (L-aC-PC25) were characterized by higher storage stability judged by their hydrodynamic radius evolution yet enhanced deposition of blood plasma opsonins on their surface according to SDS-PAGE and immunoblotting. Notably, inhibition of tubulin polymerization was found to require that the prodrug should be hydrolyzed to the parent allocolchicinoid. The L-aC-PC10 and L-aC-PC25 formulations demonstrated similar tubulin polymerization inhibition and cytotoxic activities. The L-aC-PC10 formulation should be beneficial for applications requiring liposome accumulation at tumor or inflammation sites.
Decoyinine Induced Resistance in Rice against Small Brown Planthopper Laodelphax striatellus
Authors: Shah Amir Zaman , Ma Chao , Zhang Yuanyuan , Zhang Qiuxin , Xu Gang , Yang Guoqing
Published: January 2022

Induced resistance against SBPH via microbial pesticides is considered as an eco-friendly and promising management approach. In this study, the induced resistance against SBPH in rice seedling by a new potential microbial pesticide, decoyinine (DCY), a secondary metabolite produced by Streptomyces hygroscopicus, was evaluated to investigate the effects of DCY on SBPH’s biological and population parameters along with defense-related physiological and biochemical indices in rice against SBPH feeding. We found that DCY has potential to improve rice resistance and significantly reduced the fecundity of SBPH. Laboratory results revealed that DCY treated rice significantly changed SBPH’s fecundity and population life table parameters. The concentrations of hydrogen peroxide (H2O2), soluble sugars and malondialdehyde (MDA) were significantly lower in DCY treated rice plants against SBPH infestation at 24, 48 and 96 hours post infestation (hpi), respectively. The concentrations of antioxidant enzymes, catalase (CAT) was significantly higher at 72 hpi, while super oxidase dismutase (SOD) and peroxidase (POD) concentrations were recorded higher at 96 hpi. The concentrations of synthases enzymes, phenyl alanine ammonia-lyase (PAL) was higher at 48 hpi, whereas polyphenol oxidase (PPO) concentration was maximum at 72 hpi against SBPH infestation. The results imply that DCY has unique properties to enhance rice resistance against SBPH by stimulating plant defensive responses. Microbial pesticides may be developed as an alternative to chemical pest control.
A Review of Franklinothrips vespiformis (Thysanoptera: Aeolothripidae): Life History, Distribution, and Prospects as a Biological Control Agent
Authors: Hussain Mubasher , Wang Zhaohong , Arthurs Steven P. , Gao Jing , Ye Fengxian , Chen Lingling , Mao Runqian
Published: January 2022

Predatory species comprise a small but important and often overlooked component of the Thysanoptera. A case in point, the ant-mimicking Franklinothrips are widely distributed in the tropics and are considered important generalist natural enemies for thrips and some other small arthropod prey. Franklinothrips present an addition to biocontrol applications, i.e., greenhouse or commercial application for certain target pests and situations. Current knowledge, including distribution, biological features, life history pa rameters, prey specificity, host plant associations and lass production is yet insufficient to decide to what extent Franklinothrips could contribute for biological control programs. In this review, we summarized the geographical background, morphology, and prey associations, with a focus on F. vespiformis, the most widely distributed species of predatory thrips. This literature review serves as the basis for future research into the use of Franklinothrips as biocontrol agents for economically significant insect and mite pests in China and elsewhere.
Impact of BMI, Physical Activity, and Sitting Time Levels on Health-Related Outcomes in a Group of Overweight and Obese Adults with and without Type 2 Diabetes
Authors: Pippi Roberto , Cugusi Lucia , Bergamin Marco , Bini Vittorio , Fanelli Carmine Giuseppe , Bullo Valentina , Gobbo Stefano , Di Blasio Andrea
Published: January 2022

Physical activity level and sedentary behaviors affect health status in people with obesity and type 2 diabetes (DM2); their assessment is mandatory to properly prescribe exercise programs. From January 2011 to February 2014, 293 overweight/obese adults (165 women and 128 men, mean age of 51.9 ± 9.5 years and 54.6 ± 8.3 years, respectively), with and without DM2, participated in a three-month intensive exercise program. Before starting, participants were allocated into three subgroups (overweight, body mass index or BMI = 25–29.9; class 1 of obesity, BMI = 30–34.4; or class 2 (or superior) of obesity, BMI > 35). The international physical activity questionnaire (IPAQ-it) was used to evaluate participants’ baseline sitting time (SIT) and physical activity level (PAL). Stratified multiple analyses were performed using four subgroups of SIT level according to Ekelund et al., 2016 (low, 8 h/day of SIT) and three subgroups for PAL (high, moderate, and low). Health-related measures such as anthropometric variables, body composition, hematic parameters, blood pressure values, and functional capacities were studied at the beginning and at the end of the training period. An overall improvement of PAL was observed in the entire sample following the three-month intensive exercise program together with a general improvement in several health-related measures. The BMI group factor influenced the VO2 max variations, leg press values, triglycerides, and anthropometric variables, while the SIT group factor impacted the sitting time, VO2 max, glycemic profile, and fat mass. In this study, baseline PAL and SIT did not seem to influence the effects of an exercise intervention. The characteristics of our educational program, which also included a physical exercise protocol, allowed us to obtain positive results.
Strahler Ordering Analyses on Branching Coral Canopies: Stylophora pistillata as a Case Study
Authors: Shmuel Yaniv , Ziv Yaron , Rinkevich Baruch
Published: January 2022

The three-dimensional structural complexities generated by living sessile organisms, such as trees and branching corals, embrace distinct communities of dwelling organisms, many of which are adapted to specific niches within the structure. Thus, characterizing the build-up rules and the canopy compartments may clarify small-scale biodiversity patterns and rules for canopy constituents. While biodiversity within tree canopies is usually typified by the vertical axis that is delineated by its main compartments (understory, trunk, crown), traditional studies of coral canopy dwelling species are evaluated only by viewing the whole coral head as a single homogeneous geometric structure. Here, we employ the Strahler number of a mathematical tree for the numerical measurements of the coral’s canopy complexity. We use the branching Indo-Pacific coral species Stylophora pistillata as a model case, revealing five compartments in the whole coral canopy volume (Understory, Base, Middle, Up, and Bifurcation nods). Then, the coral’s dwellers’ diel distribution patterns were quantified and analyzed. We observed 114 natal colonies, containing 32 dwelling species (11 sessile), totaling 1019 individuals during day observations, and 1359 at night (1–41 individuals/colony). Biodiversity and abundance associated with Strahler numbers, diel richness, abundance, and patterns for compartmental distributions differed significantly between day/night. These results demonstrate that the coral-canopy Strahler number is an applicable new tool for assessing canopy landscapes and canopy associated species biodiversity, including the canopy-compartmental utilization by mobile organisms during day/night and young/adult behaviors.
Ca2+ Signalling Differentially Regulates Germ-Tube Formation and Cell Fusion in Fusarium oxysporum
Authors: Kurian Smija M. , Lichius Alexander , Read Nick D.
Published: January 2022

Fusarium oxysporum is an important plant pathogen and an emerging opportunistic human pathogen. Germination of conidial spores and their fusion via conidial anastomosis tubes (CATs) are significant events during colony establishment in culture and on host plants and, hence, very likely on human epithelia. CAT fusion exhibited by conidial germlings of Fusarium species has been postulated to facilitate mitotic recombination, leading to heterokaryon formation and strains with varied genotypes and potentially increased virulence. Ca2+ signalling is key to many of the important physiological processes in filamentous fungi. Here, we tested pharmacological agents with defined modes of action in modulation of the mammalian Ca2+ signalling machinery for their effect on germination and CAT-mediated cell fusion in F. oxysporum. We found various drug-specific and dose-dependent effects. Inhibition of calcineurin by FK506 or cyclosporin A, as well as chelation of extracellular Ca2+ by BAPTA, exclusively inhibit CAT induction but not germ-tube formation. On the other hand, inhibition of Ca2+ channels by verapamil, calmodulin inhibition by calmidazolium, and inhibition of mitochondrial calcium uniporters by RU360 inhibited both CAT induction and germ-tube formation. Thapsigargin, an inhibitor of mammalian sarco/endoplasmic reticulum Ca2+ ATPase (SERCA), partially inhibited CAT induction but had no effect on germ-tube formation. These results provide initial evidence for morphologically defining roles of Ca2+-signalling components in the early developmental stages of F. oxysporum colony establishment—most notably, the indication that calcium ions act as self-signalling molecules in this process. Our findings contribute an important first step towards the identification of Ca2+ inhibitors with fungas-specific effects that could be exploited for the treatment of infected plants and humans.
Torque Generation of the Endodontic Instruments: A Narrative Review
Authors: Kwak Sang Won , Shen Ya , Liu He , Kim Hyeon-Cheol , Haapasalo Markus
Published: January 2022

As the use of nickel-titanium (NiTi) file systems for root canal therapy has become popular; hence, knowledge and understanding of the characteristics of NiTi files is essential for dentists. Unintended sudden fracture can occur during root canal shaping, and it is important to understand the conditions that may cause instrument fractures. Torque is defined as the force required to rotate the NiTi file and can be considered of as a parameter for the stress generated. The endodontic engine maintains a constant rotational speed by adjusting torque regardless of the root canal conditions. The process of root canal shaping by rotary instruments is a series of actions that requires torque and generates stress to both the teeth and the NiTi instruments. The generated stress may induce the strain accumulation on NiTi instrument and the canal wall and lead to the development of microcrack in the instrument and dentinal wall. Therefore, understanding of torque and stress generated is important to prevent the fractures to the instrument and the teeth. This stress has been measured using various experimental approaches, including microcrack observation by using a microscope or computed tomography, attaching strain gauges to the teeth, and finite element analysis. This review focuses on the stress generated to the teeth and the instrument during instrumentation under various experimental conditions. The factors related to torque generation are also discussed.
Bioconjugation Strategy for Ceramic Membranes Decorated with Candida Antarctica Lipase B—Impact of Immobilization Process on Material Features
Authors: Kujawa Joanna , Głodek Marta , Koter Izabela , Li Guoqiang , Knozowska Katarzyna , Kujawski Wojciech
Published: January 2022

A strategy for the bioconjugation of the enzyme Candida antarctica lipase B onto titania ceramic membranes with varied pore sizes (15, 50, 150, and 300 kDa) was successfully performed. The relationship between the membrane morphology, i.e.,the pore size of the ceramic support, and bioconjugation performance was considered. Owing to the dimension of the enzyme (~33 kDa), the morphology of the ceramics allowed (50, 150, and 300 kDa) or did not allow (15 kDa) the entrance of the enzyme molecules into the porous structure. Such a strategy made it possible to better understand the changes in the material (morphology) and physicochemical features (wettability, adhesiveness, and surface charge) of the samples, which were systematically examined. The silane functionalization and enzyme immobilization were accomplished via the covalent route. The samples were characterized after each stage of the modification, which was very informative from the material point of view. As a consequence of the modification, significant changes in the contact angle, roughness, adhesion, and zeta potential were observed. For instance, for the 50 kDa membrane, the contact angle increased from 29.1 ± 1.5° for the pristine sample to 72.3 ± 1.5° after silane attachment; subsequently, it was reduced to 57.2 ± 1.5° after the enzyme immobilization. Finally, the contact angle of the bioconjugated membrane used in the enzymatic process rose to 92.9 ± 1.5°. By roughness (Sq) controlling, the following amendments were noticed: for the pristine 50 kDa membrane, Sq = 1.87 ± 0.21 µm; after silanization, Sq = 2.33 ± 0.30 µm; after enzyme immobilization, Sq = 2.74 ± 0.26 µm; and eventually, after the enzymatic process, Sq = 2.37 ± 0.27 µm. The adhesion work of the 50 kDa samples was equal to 136.41 ± 2.20 mN m−1 (pristine membrane), 94.93 ± 2.00 mN m−1 (with silane), 112.24 ± 1.90 mN m−1 (with silane and enzyme), and finally, 69.12 ± 1.40 mN m−1 (after the enzymatic process). The materials and physicochemical features changed substantially, particularly after the application of the membrane in the enzymatic process. Moreover, the impact of ceramic material morphology on the zeta potential value is here presented for the first time. With an increase in the ceramic support cut-off, the amount of immobilized lipase rose, but the specific productivity was higher for membranes possessing smaller pores, owing to the higher grafting density. For the enzymatic process, two modes of accomplishment were selected, i.e., stirred-tank and cross-flow. The latter method was characterized by a much higher effectiveness, with a resulting productivity equal to 99.7 and 60.3 µmol h−1 for the 300 and 15 kD membranes, respectively.
Study of Rolling Motion of Ships in Random Beam Seas with Nonlinear Restoring Moment and Damping Effects Using Neuroevolutionary Technique
Authors: Khan Naveed Ahmad , Sulaiman Muhammad , Tavera Romero Carlos Andrés , Laouini Ghaylen , Alshammari Fahad Sameer
Published: January 2022

In this paper, a mathematical model for the rolling motion of ships in random beam seas has been investigated. The ships’ steady-state rolling motion with a nonlinear restoring moment and damping effect is modeled by the nonlinear second-order differential equation. Furthermore, an artificial neural network (NN)-based, backpropagated Levenberg-Marquardt (LM) algorithm is utilized to interpret a numerical solution for the roll angle (x(t)), velocity (x′(t)), and acceleration (x′′(t)) of the ship in random beam seas. A reference data set based on numerical examples of the mathematical model for a rolling ship for the LM-NN algorithm is generated by the numerical solver Runge–Kutta method of order 4 (RK-4). The LM-NN algorithm further uses the created data set for the validation, testing, and training of approximate solutions. The outcomes of the design paradigm are compared with those of the homotopy perturbation method (HPM), optimal homotopy analysis method (OHAM), and RK-4. Statistical analyses of the mean square error (MSE), regression, error histograms, proportional performance, and computational complexity further validate the worth of the LM-NN algorithm.
An Overview Regarding Microbial Aspects of Production and Applications of Bacterial Cellulose
Authors: Lupașcu Raluca Elisabeta , Ghica Mihaela Violeta , Dinu-Pîrvu Cristina-Elena , Popa Lăcrămioara , Velescu Bruno Ștefan , Arsene Andreea Letiția
Published: January 2022

Cellulose is the most widely used biopolymer, accounting for about 1.5 trillion tons of annual production on Earth. Bacterial cellulose (BC) is a form produced by different species of bacteria, representing a purified form of cellulose. The structure of bacterial cellulose consists of glucose monomers that give it excellent properties for different medical applications (unique nanostructure, high water holding capacity, high degree of polymerization, high mechanical strength, and high crystallinity). These properties differ depending on the cellulose-producing bacteria. The most discussed topic is related to the use of bacterial cellulose as a versatile biopolymer for wound dressing applications. The aim of this review is to present the microbial aspects of BC production and potential applications in development of value-added products, especially for biomedical applications.
Synthesis and Potential Applications of Lipid Nanoparticles in Medicine
Authors: Musielak Ewelina , Feliczak-Guzik Agnieszka , Nowak Izabela
Published: January 2022

Currently, carriers of active ingredients in the form of particles of a size measured in nanometers are the focus of interest of research centers worldwide. So far, submicrometer emulsions, liposomes, as well as microspheres, and nanospheres made of biodegradable polymers have been used in medicine. Recent studies show particular interest in nanoparticles based on lipids, and at the present time, are even referred to as the “era of lipid carriers”. With the passage of time, lipid nanoparticles of the so-called first and second generation, SLN (Solid Lipid Nanoparticles) and nanostructured lipid carriers and NLC (Nanostructured Lipid Carriers), respectively, turned out to be an alternative for all imperfections of earlier carriers. These carriers are characterized by a number of beneficial functional properties, including, among others, structure based on lipids well tolerated by the human body, high stability, and ability to carry hydro- and lipophilic compounds. Additionally, these carriers can enhance the distribution of the drug in the target organ and alter the pharmacokinetic properties of the drug carriers to enhance the medical effect and minimize adverse side effects. This work is focused on the current review of the state-of-the-art related to the synthesis and applications of popular nanoparticles in medicine, with a focus on their use, e.g., in COVID-19 vaccines.