bluuebunny/crossref_metadata_2025 huggingface dataset:
Innovation or replication? Crossing and criss-crossing in social science
Authors: Strathern Marilyn
Published: December 2012

What does it mean to find echoes of an innovatory moment in the past, or a discipline’s cutting edge in another’s worn down tool, or people in different fields quite unknown to one another following a similar intellectual trajectory over the same three or four years? A short case study of what looks uncannily like ‘independent invention’ is prefaced by reflections on replicatory practice in the social sciences. Some US sociological theorizing on the workings of fractal distinctions within disciplines, specifically across many foundational arguments in social science, finds a counterpart in UK anthropological theorizing on scale and replication in social phenomena at large. The conjunction is amusing; it could well be instructive. In any event, there is a challenge here to comparisons across disciplines.
Plasma Viscosity
Authors: Aras Sevgi , Tek Ibrahim , Varli Murat , Yalcin Ahmet , Cengiz Ozlem Karaarslan , Atmis Volkan , Atli Teslime
Published: December 2012

In this study, the importance of plasma viscosity (PV) as a biomarker in differential diagnosis of dementia subtypes especially Alzheimer’s disease (AD) and vascular dementia (VaD) was investigated. Our study recruited 45 patients with AD, 35 patients with VaD, and control participants. Individuals with inflammatory disease, infection, heart, liver, renal failure, and with high erythrocyte sedimentation rate and C-reactive protein levels were excluded from the study. The cases underwent comprehensive geriatric assessment. The PV measurements were performed with Brookfield DV-II viscometer. The PV measurements of AD, VaD, and control groups were 1.61 ± 0.08, 1.70 ± 0.06, and 1.48 ± 0.06 mPa S, respectively. The PV levels of the dementia group were significantly higher than the control group ( P < .001). When the dementia group was analyzed by itself, patients with VaD had higher PV levels than the patients with AD ( P < .001). The PV is a biomarker to be used in diagnosis as well as in differentiating between the 2 most common forms of dementia which are AD and VaD.
An In Vitro and In Vivo Investigation of the Antimetastatic Effects of a Chinese Medicinal Decoction, Erxian Decoction, on Human Ovarian Cancer Models
Authors: Chu Ellie S. M. , Sze Stephen C. W. , Cheung Ho P. , Liu Qing , Ng Tzi B. , Tong Yao
Published: December 2012

Objectives. Erxian Decoction (EXD) is a well-documented Chinese medicinal formulation, which has been clinically applied for years for relieving menopausal syndromes by modulating hormonal levels indicating that EXD might also be effective in treating hormone-related tumors. This study aimed to differentially investigate the efficacy of EXD and its antimetastatic property on human ovarian cancer cells, OVCA429. Methods. The efficacy and cell cycle progression of EXD on OVCA429 cells was determined by MTT assay and flow cytometry, respectively. The modulated expression of metastatic markers by EXD in OVCA429 cells and xenografts was evaluated at transcriptional and translational levels by Western blotting and real-time polymerase chain reaction, respectively. The migrating and invasive ability of the cancer cells were determined by wound healing and invasive assays. Results. The IC50 value of EXD on OVCA429 cells was determined after 24 hours incubation with EXD at 1 mg/mL. EXD (1.5 mg/mL) mediated S-phase cell cycle arrest and apoptotic cell death at 24 hours posttreatment. EXD repressed the expression of several metastatic mediators, including EGFR, ErbB2, MMP2, MMP7, MMP9, and VEGF in OVCA429 cells and xenografts at transcriptional and/or translational levels. Furthermore, EXD functionally demonstrated significant inhibition of migrating and invasive ability of OVCA429 cells. EXD suppressed tumor size in xenografts without any adverse effects on body weight. Conclusions. This is the first study that illustrates the antimetastatic property of EXD on human ovarian cancer models. This decoction merits serious consideration for further delineation of its multiple pharmacological effects, especially on hormone-related cancers, and these would be valuable for future clinical applications of EXD as an alternative regime for cancers.
Online Instruction As Innovation in an Educational Leadership Program
Authors: Owens Michael A.
Published: December 2012

This case was developed for use in a course in educational leadership or organizational theory with a focus on change in educational organizations. In this case narrative, data are presented relating to a proposed restructuring of an educational leadership program. Students should analyze the narrative to look for information that helps them identify internal and external factors that lead to and inhibit change and innovation. In addition, students should identify issues faced by program-level leaders who, as middle managers, must respond to both leaders and followers.
It’s a Different World at the High School
Authors: Hackmann Donald G.
Published: December 2012

This case describes initial conflicts experienced by a new superintendent that involve faculty resistance and diminished academic expectations at the high school, compared with the district’s other schools. Compounding the situation is the fact that the Board of Education was bitterly divided when reaching their superintendent hiring decision, with several members supportive of hiring the current high school principal. Fueled by the discovery of numerous parent complaints regarding discriminatory treatment of their children, the superintendent is confronted by the high school faculty who aggressively defend their practices and a high school principal who appears to be undermining her authority.
‘Just give me the best quality of life questionnaire’: the Karnofsky scale and the history of quality of life measurements in cancer trials
Authors: Timmermann Carsten
Published: December 2012

Objectives:

To use the history of the Karnofsky Performance Scale as a case study illustrating the emergence of interest in the measurement and standardisation of quality of life; to understand the origins of current-day practices.

Methods:

Articles referring to the Karnofsky scale and quality of life measurements published from the 1940s to the 1990s were identified by searching databases and screening journals, and analysed using close-reading techniques. Secondary literature was consulted to understand the context in which articles were written.

Results:

The Karnofsky scale was devised for a different purpose than measuring quality of life: as a standardisation device that helped quantify effects of chemotherapeutic agents less easily measurable than survival time. Interest in measuring quality of life only emerged around 1970.

Discussion:

When quality of life measurements were increasingly widely discussed in the medical press from the late 1970s onwards, a consensus emerged that the Karnofsky scale was not a very good tool. More sophisticated approaches were developed, but Karnofsky continued to be used. I argue that the scale provided a quick and simple, approximate assessment of the ‘soft’ effects of treatment by physicians, overlapping but not identical with quality of life.
The air raids that never were and the war that nobody won: Government propaganda in conflict reporting and how journalists should respond to it
Authors: Rodgers James
Published: December 2012

During the bombing campaign against Libya in the spring of 2011, the fog of war enveloped not only the shifting frontlines of the battles between forces loyal to the then Libyan leader, Muammar Gaddafi, but also the more comfortable surroundings of British government ministries. A news story of an aborted air raid reflected the huge importance which governments increasingly place upon getting their message across in the international media in times of conflict. Looking at that example from the conflict in Libya, and studying in detail others from Russia’s 2008 war with Georgia, this article argues that journalists must correspondingly be increasingly aware of attempts by governments, and their hired public relations hands, to influence reporting.
Intermedia agenda-setting in the age of globalization: A multinational agenda-setting test
Authors: Du Ying Roselyn
Published: December 2012

This study explores mass media’s agenda-setting function in a context of increased globalization to determine whether the theory, which was built upon intra-nation environments, functions in the global setting. It matches public agendas with media agendas to investigate agenda-setting effects in 11 countries worldwide, then compares media agendas across countries to consider whether inter-nation intermedia influence exists. Results suggest a general pattern of the agenda-setting function of mass media in the countries examined. The study finds evidence of inter-nation intermedia influence and thus presents a new way to look at the intermedia agenda-setting relationship – moving this research from comparisons within a local area to cross-national intermedia comparisons. Furthermore, this study checks for evidence of directional inter-nation intermedia agenda-setting. The findings suggest a complex inter-nation intermedia relationship and imply that, in the age of globalization, the simple ‘powerful West’ reasoning derived from the old days may need to be rethought and updated.
Mediating fear
Authors: Jeffries Fiona
Published: December 2012

This paper proposes a critical contribution to the contemporary debates around the ‘culture of fear’ in media and communication studies. It seeks to open up new lines of inquiry into the political significance of fear in the production of everyday life and subjectivity in the context of hyper-mediatized global modernity. Drawing on Jesús Martín-Barbero’s concept of ‘mediations’, the paper aims to contribute to the vital critical communication and media studies scholarship on the culture of fear by proposing the incorporation of analyses of the diverse and creative struggles of social movements working to subvert the political uses of fear in the dominant public sphere.
“What a Glorious Moment in Jurisprudence”: Rhetoric, Law, and Battlestar Galactica
Authors: Kapica Steven S.
Published: December 2012

This article is an inquiry into the legal rhetoric and jurisprudential tensions presented in television series Battlestar Galactica ( BSG) (2003–2009). Gaius Baltar’s trial for treason, which closes the series’ third season, serves as a compelling rumination on collective guilt, vengeance, and injustice, succinctly encapsulating BSG ethos. This study, drawing on rhetorical scholarship, explores the trial’s performative features and through rhetorical analysis illustrates how the legal actors in Baltar’s trial frame justice, set legal precedent, and re-establish community-based order in a tenuous Galactica universe – a universe particularly revealing of post-9/11 anxieties and American jurisprudence in crisis.
Four types of coping with COPD-induced breathlessness in daily living: a grounded theory study
Authors: Jørgensen Lene Bastrup , Dahl Ronald , Pedersen Preben Ulrich , Lomborg Kirsten
Published: December 2012

Coping with breathlessness is a complex and multidimensional challenge for people with chronic obstructive pulmonary disease (COPD) and involves interacting physiological, cognitive, affective, and psychosocial dimensions. The aim of this study was to explore how people with moderate to most severe COPD predominantly cope with breathlessness during daily living. We chose a multi-modal grounded theory design that holds the opportunity to combine qualitative and quantitative data to capture and explain the multidimensional coping behaviour among people with COPD. The participants’ main concern in coping with breathlessness appeared to be an endless striving to economise on resources in an effort to preserve their integrity. In this integrity-preserving process, four predominant coping types emerged and were labelled: ‘Overrater’, ‘Challenger’, ‘Underrater’, and ‘Leveller’. Each coping type comprised distinctive physiological, cognitive, affective and psychosocial features constituting coping-type-specific indicators.

In theory, four predominant coping types with distinct physiological, cognitive, affective and psychosocial properties are observed among people with COPD. The four coping types seem to constitute a coping trajectory. This hypothesis should be further tested in a longitudinal study.
Clinical findings in C5-C6 and C5-C7 root palsies with brachial plexus traction lesions
Authors: Bertelli J. A. , Ghizoni M. F.
Published: December 2012

Stretch injuries of the C5-C7 roots of the brachial plexus traditionally have been associated with palsies of shoulder abduction/external rotation, elbow flexion/extension, and wrist, thumb, and finger extension. Based on current myotome maps we hypothesized that, as far as motion is concerned, palsies involving C5-C6 and C5-C7 root injuries should be similar. In 38 patients with upper-type palsies of the brachial plexus, we examined for correlations between clinical findings and root injury level, as documented by CT tomomyeloscan. Contrary to commonly held beliefs, C5-C7 root injuries were not associated with loss of extension of the elbow, wrist, thumb, or fingers, but residual hand strength was much lower with C5-C7 vs C5-C6 lesions.
Statin therapy and the risk for diabetes among adult women: do the benefits outweigh the risk?
Authors: Ma Yunsheng , Culver Annie , Rossouw Jacques , Olendzki Barbara , Merriam Philip , Lian Bill , Ockene Ira
Published: December 2012

Purpose of review:

The purpose of this review was to examine statin therapy and the risk for diabetes among adult women using a selective review.

Recent findings:

The literature contains reports of new-onset diabetes associated with statin use. While many studies do not report sex-specific results, there is evidence indicating the risk to benefit ratio may vary by gender. However, the absolute effects are not clear because women have historically been under-represented in clinical trials.

A review of the literature indicates that the cardiovascular benefits of statins appear to outweigh the risk for statin-related diabetes. However, the effect may depend upon baseline diabetes risk, dose, and statin potency. Rigorous, long-term studies focused on the risks and benefits of statins in women are unavailable to sort for gender-specific differences. Until this changes, individualized attention to risk assessment, and strong prevention with lifestyle changes must prevail.
The new patent foramen ovale occluder FIGULLA in complex septal anatomy: a case series
Authors: Baglini Roberto , Baldari Duccio , Amaducci Andrea , D’Ancona Giuseppe
Published: December 2012

Background:

The appropriate treatment strategy for secondary stroke prevention in patients with cryptogenic stroke and patent foramen ovale (PFO) remains challenging. The aim of this study was to describe a case series of patients with PFO and complex septal anatomy who underwent percutaneous correction using a FIGULLA (Occlutech®) septal occluder (FSO).

Patients:

Ten consecutive patients (6 females, 4 males, mean age 41.6 ± 16.0 years, range 17–52 years; Group 1) with cryptogenetic stroke and/or transient cerebral ischemia and complex septal anatomy, as defined by intraprocedural transesophageal echocardiogram (TEE) were compared with a group of 25 patients (10 females, 15 males, mean age 43.7 ± 12.3 years; Group 2) with usual tunnel-like PFO anatomy in whom PFO was closed by an Amplatzer septal occluder (ASO; AGA®).

Results:

No significant differences were noted between Group 1 and Group 2 for immediate success rate, residual intraprosthetic shunt at the end of the procedure, discharge, 1, 6 and 12 months follow up, number of attempts, procedure time, fluoroscopy time, or cardiac complication (atrial arrhythmias, device embolism). The only significant difference was shown for delivery sheath size (11 ± 2 versus 9 ± 1 F) and incidence of local hematoma (30% versus 12 %) between Group 1 and Group 2, without any clinical consequence (need of transfusion) or increase in length of stay.

Conclusion:

FSO shows high performance in patients with PFO and complex septal anatomy when compared with patients with PFO and uncomplicated atrial anatomy treated by ASO. Its favorable behavior is probably related to fabric features such as the total amount of metal and the presence of titanium.
Memory of Sponsorship-Linked Marketing Communications
Authors: Cornwell T. Bettina , Humphreys Michael S. , Quinn Emerald A. , McAlister Anna R.
Published: December 2012

Corporate sponsorship value is brought into question when false recognition of foils suggests confusion regarding true sponsors. While an indicator of confusion, recognition false alarms do not tell the entire story regarding memory for sponsor-event relationships. Two free recall experiments show relatively good memory for sponsors and also that under certain conditions, the mention of direct competitors can actually facilitate recall of true sponsors and events. The findings point to the importance of understanding the memory-based characteristics of measurement as well as to the memory-supported decision-making tasks that sponsorship information might eventually influence.
FACS analysis of Stat3/5 signaling reveals sensitivity to G-CSF and IL-6 as a significant prognostic factor in pediatric AML: a Children's Oncology Group report
Authors: Redell Michele S. , Ruiz Marcos J. , Gerbing Robert B. , Alonzo Todd A. , Lange Beverly J. , Tweardy David J. , Meshinchi Soheil
Published: December 2012

Key Points

In pediatric AML, patterns of Stat3 activation by G-CSF and IL-6 can be identified that are associated with survival. Such functional information may be useful for risk assessment and for determining which patients may benefit from alternative therapies.
Association of telomere instability with senescence of porcine cells
Authors: Ji Guangzhen , Liu Kai , Okuka Maja , Liu Na , Liu Lin
Published: December 2012

Background

Telomeres are essential for the maintenance of genomic stability, and telomere dysfunction leads to cellular senescence, carcinogenesis, aging, and age-related diseases in humans. Pigs have become increasingly important large animal models for preclinical tests and study of human diseases, and also may provide xeno-transplantation sources. Thus far, Southern blot analysis has been used to estimate average telomere lengths in pigs. Telomere quantitative fluorescence in situ hybridization (Q-FISH), however, can reveal status of individual telomeres in fewer cells, in addition to quantifying relative telomere lengths, and has been commonly used for study of telomere function of mouse and human cells. We attempted to investigate telomere characteristics of porcine cells using telomere Q-FISH method.

Results

The average telomere lengths in porcine cells measured by Q-FISH correlated with those of quantitative real-time PCR method (qPCR) or telomere restriction fragments (TRFs) by Southern blot analysis. Unexpectedly, we found that porcine cells exhibited high incidence of telomere doublets revealed by Q-FISH method, coincided with increased frequency of cellular senescence. Also, telomeres shortened during subculture of various porcine primary cell types. Interestingly, the high frequency of porcine telomere doublets and telomere loss was associated with telomere dysfunction-induced foci (TIFs). The incidence of TIFs, telomere doublets and telomere loss increased with telomere shortening and cellular senescence during subculture.

Conclusion

Q-FISH method using telomere PNA probe is particularly useful for characterization of porcine telomeres. Porcine cells exhibit high frequency of telomere instability and are susceptible to telomere damage and replicative senescence.
A genome-wide scan of selective sweeps in two broiler chicken lines divergently selected for abdominal fat content
Authors: Zhang Hui , Wang Shou-Zhi , Wang Zhi-Peng , Da Yang , Wang Ning , Hu Xiao-Xiang , Zhang Yuan-Dan , Wang Yu-Xiang , Leng Li , Tang Zhi-Quan , Li Hui
Published: December 2012

Background

Genomic regions controlling abdominal fatness (AF) were studied in the Northeast Agricultural University broiler line divergently selected for AF. In this study, the chicken 60KSNP chip and extended haplotype homozygosity (EHH) test were used to detect genome-wide signatures of AF.

Results

A total of 5357 and 5593 core regions were detected in the lean and fat lines, and 51 and 57 reached a significant level (P<0.01), respectively. A number of genes in the significant core regions, includingRB1,BBS7,MAOA,MAOB,EHBP1,LRP2BP,LRP1B,MYO7A,MYO9AandPRPSAP1, were detected. These genes may be important for AF deposition in chickens.

Conclusions

We provide a genome-wide map of selection signatures in the chicken genome, and make a contribution to the better understanding the mechanisms of selection for AF content in chickens. The selection for low AF in commercial breeding using this information will accelerate the breeding progress.
A role for prenylated rab acceptor 1 in vertebrate photoreceptor development
Authors: Dickison Virginia M , Richmond Angela M , Abu Irqeba Ameair , Martak Joshua G , Hoge Sean CE , Brooks Matthew J , Othman Mohammed I , Khanna Ritu , Mears Alan J , Chowdhury Adnan Y , Swaroop Anand , Ogilvie Judith Mosinger
Published: December 2012

Background

The rd1 mouse retina is a well-studied model of retinal degeneration where rod photoreceptors undergo cell death beginning at postnatal day (P) 10 until P21. This period coincides with photoreceptor terminal differentiation in a normal retina. We have used the rd1 retina as a model to investigate early molecular defects in developing rod photoreceptors prior to the onset of degeneration.

Results

Using a microarray approach, we performed gene profiling comparing rd1 and wild type (wt) retinas at four time points starting at P2, prior to any obvious biochemical or morphological differences, and concluding at P8, prior to the initiation of cell death. Of the 143 identified differentially expressed genes, we focused on Rab acceptor 1 (Rabac1), which codes for the protein Prenylated rab acceptor 1 (PRA1) and plays an important role in vesicular trafficking. Quantitative RT-PCR analysis confirmed reduced expression of PRA1 in rd1 retina at all time points examined. Immunohistochemical observation showed that PRA1-like immunoreactivity (LIR) co-localized with the cis-Golgi marker GM-130 in the photoreceptor as the Golgi translocated from the perikarya to the inner segment during photoreceptor differentiation in wt retinas. Diffuse PRA1-LIR, distinct from the Golgi marker, was seen in the distal inner segment of wt photoreceptors starting at P8. Both plexiform layers contained PRA1 positive punctae independent of GM-130 staining during postnatal development. In the inner retina, PRA1-LIR also colocalized with the Golgi marker in the perinuclear region of most cells. A similar pattern was seen in the rd1 mouse inner retina. However, punctate and significantly reduced PRA1-LIR was present throughout the developing rd1 inner segment, consistent with delayed photoreceptor development and abnormalities in Golgi sorting and vesicular trafficking.

Conclusions

We have identified genes that are differentially regulated in the rd1 retina at early time points, which may give insights into developmental defects that precede photoreceptor cell death. This is the first report of PRA1 expression in the retina. Our data support the hypothesis that PRA1 plays an important role in vesicular trafficking between the Golgi and cilia in differentiating and mature rod photoreceptors.
A Tourist-like MITE insertion in the upstream region of the BnFLC.A10 gene is associated with vernalization requirement in rapeseed (Brassica napus L.)
Authors: Hou Jinna , Long Yan , Raman Harsh , Zou Xiaoxiao , Wang Jing , Dai Shutao , Xiao Qinqin , Li Cong , Fan Longjiang , Liu Bin , Meng Jinling
Published: December 2012

Background

Rapeseed (Brassica napus L.) has spring and winter genotypes adapted to different growing seasons. Winter genotypes do not flower before the onset of winter, thus leading to a longer vegetative growth period that promotes the accumulation and allocation of more resources to seed production. The development of winter genotypes enabled the rapeseed to spread rapidly from southern to northern Europe and other temperate regions of the world. The molecular basis underlying the evolutionary transition from spring- to winter- type rapeseed is not known, however, and needs to be elucidated.

Results

We fine-mapped the spring environment specific quantitative trait locus (QTL) for flowering time, qFT10-4,in a doubled haploid (DH) mapping population of rapeseed derived from a cross between Tapidor (winter-type) and Ningyou7 (semi-winter) and delimited the qFT10-4 to an 80-kb region on chromosome A10 of B. napus. The BnFLC.A10 gene, an ortholog of FLOWERING LOCUS C (FLC) in Arabidopsis, was cloned from the QTL. We identified 12 polymorphic sites between BnFLC.A10 parental alleles of the TN-DH population in the upstream region and in intron 1. Expression of both BnFLC.A10 alleles decreased during vernalization, but decreased more slowly in the winter parent Tapidor. Haplotyping and association analysis showed that one of the polymorphic sites upstream of BnFLC.A10 is strongly associated with the vernalization requirement of rapeseed (r
2
= 0.93, χ

2
= 0.50). This polymorphic site is derived from a Tourist-like miniature inverted-repeat transposable element (MITE) insertion/deletion in the upstream region of BnFLC.A10. The MITE sequence was not present in the BnFLC.A10 gene in spring-type rapeseed, nor in ancestral ‘A’ genome species B. rapa genotypes. Our results suggest that the insertion may have occurred in winter rapeseed after B. napus speciation.

Conclusions

Our findings strongly suggest that (i) BnFLC.A10 is the gene underlying qFT10-4, the QTL for phenotypic diversity of flowering time in the TN-DH population, (ii) the allelic diversity caused by MITE insertion/deletion upstream of BnFLC.A10 is one of the major causes of differentiation of winter and spring genotypes in rapeseed and (iii) winter rapeseed has evolved from spring genotypes through selection pressure at the BnFLC.A10 locus, enabling expanded cultivation of rapeseed along the route of Brassica domestication.
Thromboembolic risks of recombinant factor VIIa Use in warfarin-associated intracranial hemorrhage: a case–control study
Authors: H-Y Chou Sherry , Xuemei Cai , G Konigsberg Rachael , M Bresette Linda , V Henderson Galen , A Sorond Farzaneh , K Feske Steven
Published: December 2012

Background

Recombinant factor VIIa (rFVIIa) may be used for rapid hemostasis in life-threatening hemorrhage. In warfarin-associated intracerebral hemorrhage (wICH), FVIIa use is controversial and may carry significant thromboembolic risks. We compared incidence of baseline thromboembolic risk factors and thromboembolism rates in wICH patients treated with additional rFVIIa to those treated with standard therapy of fresh frozen plasma (FFP) and vitamin K alone.

Methods

We identified 45 consecutive wICH patients treated with additional rFVIIa over 5-year period, and 34 consecutive wICH patients treated with standard therapy alone as comparison group. We compared the incidence of post-hemorrhage cardiac and extra-cardiac thromboembolic complications between two treatment groups, and used logistic regression to adjust for significant confounders such as baseline thromboembolic risk factors. We performed secondary analysis comparing the quantity of FFP transfused between two treatment cohorts.

Results

Both rFVIIa-treated and standard therapy-treated wICH patients had a high prevalence of pre-existing thromboembolic diseases including atrial fibrillation (73% vs 68%), deep venous thrombosis (DVT) or pulmonary embolism (PE) (22% vs 18%), coronary artery disease (CAD) (38% vs 32%), and abnormal electrocardiogram (EKG) (78% vs 85%). Troponin elevation following wICH was prevalent in both groups (47% vs 41%). Clinically significant myocardial infarction (MI), defined as troponin > 1.0 ng/dL, occurred in 13% of rFVIIa-treated and 6% of standard therapy-treated patients (p=0.52). Past history of CAD (p=0.0061) and baseline abnormal EKG (p=0.02) were independently associated with clinically significant MI following wICH while rFVIIa use was not. The incidences of DVT/PE (2% vs 9%; p=0.18) and ischemic stroke (2% vs 0%; p=0.38) were similar between two treatment groups. Recombinant FVIIa-treated patients had lower mean INR at 3 (p=0.0001) and 6 hours (p<0.0001) and received fewer units of FFP transfusion (3 vs 5; p=0.003).

Conclusions

Pre-existing thromboembolic risk factors as well as post-hemorrhage troponin elevation are prevalent in wICH patients. Clinically significant MI occurs in up to 13% of wICH patients. rFVIIa use was not associated with increased incidence of clinically significant MI or other venous or arterial thromboembolic events in this wICH cohort.
Influence of interpersonal violence on maternal anxiety, depression, stress and parenting morale in the early postpartum: a community based pregnancy cohort study
Authors: Malta Lise A , McDonald Sheila W , Hegadoren Kathy M , Weller Carol A , Tough Suzanne C
Published: December 2012

Background

Research has shown that exposure to interpersonal violence is associated with poorer mental health outcomes. Understanding the impact of interpersonal violence on mental health in the early postpartum period has important implications for parenting, child development, and delivery of health services. The objective of the present study was to determine the impact of interpersonal violence on depression, anxiety, stress, and parenting morale in the early postpartum.

Methods

Women participating in a community-based prospective cohort study (n = 1319) completed questionnaires prior to 25 weeks gestation, between 34–36 weeks gestation, and at 4 months postpartum. Women were asked about current and past abuse at the late pregnancy data collection time point. Postpartum depression, anxiety, stress, and parenting morale were assessed at 4 months postpartum using the Edinburgh Postnatal Depression Scale, the Spielberger State Anxiety Index, the Cohen Perceived Stress Scale, and the Parenting Morale Index, respectively. The relationship between interpersonal violence and postpartum psychosocial health status was examined using Chi-square analysis (p < 0.05) and multivariable logistic regression.

Results

Approximately 30% of women reported one or more experience of interpersonal violence. Sixteen percent of women reported exposure to child maltreatment, 12% reported intimate partner violence, and 12% reported other abuse. Multivariable logistic regression analysis found that a history of child maltreatment had an independent effect on depression in the postpartum, while both child maltreatment and intimate partner violence were associated with low parenting morale. Interpersonal violence did not have an independent effect on anxiety or stress in the postpartum.

Conclusion

The most robust relationships were seen for the influence of child maltreatment on postpartum depression and low parenting morale. By identifying women at risk for depression and low parenting morale, screening and treatment in the prenatal period could have far-reaching effects on postpartum mental health thus benefiting new mothers and their families in the long term.
Environments For Healthy Living (EFHL) Griffith birth cohort study: characteristics of sample and profile of antenatal exposures
Authors: Cameron Cate M , Scuffham Paul A , Shibl Rania , Ng ShuKay , Scott Rani , Spinks Anneliese , Mihala Gabor , Wilson Andrew , Kendall Elizabeth , Sipe Neil , McClure Roderick J
Published: December 2012

Background

The Environments for Healthy Living (EFHL) study is a repeated sample, longitudinal birth cohort in South East Queensland, Australia. We describe the sample characteristics and profile of maternal, household, and antenatal exposures. Variation and data stability over recruitment years were examined.

Methods

Four months each year from 2006, pregnant women were recruited to EFHL at routine antenatal visits on or after 24 weeks gestation, from three public maternity hospitals. Participating mothers completed a baseline questionnaire on individual, familial, social and community exposure factors. Perinatal data were extracted from hospital birth records. Descriptive statistics and measures of association were calculated comparing the EFHL birth sample with regional and national reference populations. Data stability of antenatal exposure factors was assessed across five recruitment years (2006–2010 inclusive) using the Gamma statistic for ordinal data and chi-squared for nominal data.

Results

Across five recruitment years 2,879 pregnant women were recruited which resulted in 2904 live births with 29 sets of twins. EFHL has a lower representation of early gestational babies, fewer still births and a lower percentage of low birth weight babies, when compared to regional data. The majority of women (65%) took a multivitamin supplement during pregnancy, 47% consumed alcohol, and 26% reported having smoked cigarettes. There were no differences in rates of a range of antenatal exposures across five years of recruitment, with the exception of increasing maternal pre-pregnancy weight (p=0.0349), decreasing rates of high maternal distress (p=0.0191) and decreasing alcohol consumption (p<0.0001).

Conclusions

The study sample is broadly representative of births in the region and almost all factors showed data stability over time. This study, with repeated sampling of birth cohorts over multiple years, has the potential to make important contributions to population health through evaluating longitudinal follow-up and within cohort temporal effects.

Trial registration

Australian and New Zealand Clinical Trials Registry ACTRN12610000931077
Lipoteichoic acid is an important microbe-associated molecular pattern of Lactobacillus rhamnosus GG
Authors: Claes Ingmar JJ , Segers Marijke E , Verhoeven Tine LA , Dusselier Michiel , Sels Bert F , De Keersmaecker Sigrid CJ , Vanderleyden Jos , Lebeer Sarah
Published: December 2012

Background

Probiotic bacteria are increasingly used as immunomodulatory agents. Yet detailed molecular knowledge on the immunomodulatory molecules of these bacteria is lagging behind. Lipoteichoic acid (LTA) is considered a major microbe-associated molecular pattern (MAMP) of Gram-positive bacteria. However, many details and quantitative data on its immune signalling capacity are still unknown, especially in beneficial bacteria. Recently, we have demonstrated that a dltD mutant of the model probiotic Lactobacillus rhamnosus GG (LGG), having modified LTA molecules, has an enhanced probiotic efficacy in a DSS-induced colitis model as compared to wild-type.

Results

In this study, the importance of D-alanylated and acylated LTA for the pro-inflammatory activity of LGG was studied in vitro. Purified native LTA of LGG wild-type exhibited a concentration-dependent activation of NF-κB signalling in HEK293T cells after interaction with TLR2/6, but not with TLR2 alone. Chemical deacylation of LTA interfered with the TLR2/6 interaction, while a moderate effect was observed with chemical dealanylation. Similarly, the dltD mutant of LGG exhibited a significantly reduced capacity to activate TLR2/6-dependent NF-κB signalling in a HEK293T reporter cell line compared to wild-type. In addition, the dltD mutant of LGG showed a reduced induction of mRNA of the chemokine IL-8 in the Caco-2 epithelial cell line compared to wild-type. Experiments with highly purified LTA of LGG confirmed that LTA is a crucial factor for IL-8 mRNA induction in Caco-2 epithelial cells. Chemical dealanylation and deacylation reduced IL-8 mRNA expression.

Conclusions

Taken together, our results indicate that LTA of LGG is a crucial MAMP with pro-inflammatory activities such as IL-8 induction in intestinal epithelial cells and NF-κB induction in HEK293T cells via TLR2/6 interaction. The lipid chains of LGG LTA are needed for these activities, while also the D-alanine substituents are important, especially for IL-8 induction in Caco-2 cells.
Synchronous adenocarcinomas of the colon presenting as synchronous colocolic intussusceptions in an adult
Authors: Chen Chuang-Wei , Lai Chieh-Wen , Hsiao Koung-Hong
Published: December 2012

Intussusception is uncommon in adults. To our knowledge, synchronous colocolic intussusceptions have never been reported in the literature. Here we described the case of a 59-year-old female of synchronous colocolic intussusceptions presenting as acute abdomen that was diagnosed by CT preoperatively. Laparotomy with radical right hemicolectomy and sigmoidectomy was undertaken without reduction of the invagination due to a significant risk of associated malignancy. The final diagnosis was synchronous adenocarcinoma of proximal transverse colon and sigmoid colon without lymph nodes or distant metastasis. The patient had an uneventful recovery. The case also emphasizes the importance of thorough exploration during surgery for bowel invagination since synchronous events may occur.
Role of reactive nitrogen species in male infertility
Authors: Doshi Sejal B , Khullar Karishma , Sharma Rakesh K , Agarwal Ashok
Published: December 2012

Reactive nitrogen species (RNS) is a subset of free oxygen radicals called reactive oxygen species (ROS). Physiological levels of ROS are necessary to maintain the reproductive functions such as cell signaling, tight junction regulation, production of hormones, capacitation, acrosomal reaction, sperm motility, and zona pellucida binding. However, an excess of RNS can adversely affect reproductive potential by causing testicular dysfunction, decreased gonadotropin secretion, and abnormal semen parameters. Because such levels of RNS have been demonstrated in males with fertility problems and routine semen analysis has not been able to accurately predict IVF outcomes, it is imperative that novel strategies be developed in order to both assess and treat oxidative stress. This article describes both physiological and pathological roles of this unique subset of ROS.
The perfused swine uterus model: long-term perfusion
Authors: Geisler Klaudija , Künzel Julian , Grundtner Philipp , Müller Andreas , Beckmann Matthias W , Dittrich Ralf
Published: December 2012

Background

It has previously been shown that the viability of swine uteri can be maintained within the physiological range in an open perfusion model for up to 8 hours. The aim of this study was to assess medium- to long-term perfusion of swine uteri using a modified Krebs–Ringer bicarbonate buffer solution (KRBB) in the established open perfusion model.

Methods

In an experimental study at an infertility institute, 30 swine uteri were perfused: group 1: n = 11, KRBB; group 2: n = 8, modified KRBB with drainage of perfusate supernatant; group 3: n = 11, modified KRBB with drainage of perfusate every 2 h and substitution with fresh medium. Modified and conventional KRBB were compared with regard to survival and contraction parameters: intrauterine pressure (IUP), area under the curve (AUC), and frequency of contractions (F).

Results

Modified KRBB showed significantly higher IUP, AUC, and F values than perfusion with conventional KRBB. In group 3, the organ survival time of up to 17 h, with a 98% rate of effective contraction time, differed significantly from group 1 (P < 0.001).

Conclusions

Using modified KRBB in combination with perfusate substitution improves the open model for perfusion of swine uteri with regard to survival time and quality of contraction parameters. This model can be used for medium- to long-term perfusion of swine uteri, allowing further metabolic ex vivo studies in a cost-effective way and with little logistic effort.
Hepatocyte-specific S100a8 and S100a9 transgene expression in mice causes Cxcl1 induction and systemic neutrophil enrichment
Authors: Wiechert Lars , Németh Julia , Pusterla Tobias , Bauer Christine , De Ponti Aurora , Manthey Sandra , Marhenke Silke , Vogel Arndt , Klingmüller Ursula , Hess Jochen , Angel Peter
Published: December 2012

Background

Calprotectin consists of the Ca2+-binding proteins S100a8 and S100a9 that are induced in epithelial cells in response to tissue damage and infection. Both proteins are also secreted by activated innate immune cells and numerous studies demonstrate their crucial role in pathological conditions of acute and chronic inflammation.

Results

Here, we established a conditional mouse model with simultaneousS100a8andS100a9transgene expression in hepatocytes (TgS100a8a9hep) under the control of doxycycline to unravel the role of epithelial-derived Calprotectin on tissue homeostasis and inflammation.TgS100a8a9hepmice displayed a significant enrichment of neutrophils in peripheral blood and tissues with high blood content. Interestingly,Cxcl1transcription was significantly induced in the liver ofTgS100a8a9hepmice and primary hepatocytes derived thereof as compared to Control mice, accompanied by an increase of Cxcl1 serum levels. However, expression of other chemokines with a known function in neutrophil mobilization from the bone marrow, e.g. Csf3 and Cxcl2, was not altered. Doxycycline treatment ofTgS100a8a9hepmice reducedCxcl1expression in the liver and resulted in normal numbers of neutrophils.

Conclusion

In summary, our data demonstrate for the first time that hepatocyte-specific S100a8 and S100a9 expression induces a systemic mobilization of neutrophils by a specific activation of Cxcl1 transcription in the liver.
Synthetic dye decolorization by three sources of fungal laccase
Authors: Forootanfar Hamid , Moezzi Atefeh , Aghaie-Khozani Marzieh , Mahmoudjanlou Yasaman , Ameri Alieh , Niknejad Farhad , Faramarzi Mohammad Ali
Published: December 2012

Decolorization of six synthetic dyes using three sources of fungal laccase with the origin of Aspergillus oryzae, Trametes versicolor, and Paraconiothyrium variabile was investigated. Among them, the enzyme from P. variabile was the most efficient which decolorized bromophenol blue (100%), commassie brilliant blue (91%), panseu-S (56%), Rimazol brilliant blue R (RBBR; 47%), Congo red (18.5%), and methylene blue (21.3%) after 3 h incubation in presence of hydroxybenzotriazole (HBT; 5 mM) as the laccase mediator. It was also observed that decolorization efficiency of all dyes was enhanced by increasing of HBT concentration from 0.1 mM to 5 mM. Laccase from A. oryzae was able to remove 53% of methylene blue and 26% of RBBR after 30 min incubation in absence of HBT, but the enzyme could not efficiently decolorize other dyes even in presence of 5 mM of HBT. In the case of laccase from T. versicolor, only RBBR was decolorized (93%) in absence of HBT after 3 h incubation.
Analysis of alternative signaling pathways of endoderm induction of human embryonic stem cells identifies context specific differences
Authors: Mathew Shibin , Jaramillo Maria , Zhang Xinan , Zhang Li Ang , Soto-Gutiérrez Alejandro , Banerjee Ipsita
Published: December 2012

Background

Lineage specific differentiation of human embryonic stem cells (hESCs) is largely mediated by specific growth factors and extracellular matrix molecules. Growth factors initiate a cascade of signals which control gene transcription and cell fate specification. There is a lot of interest in inducing hESCs to an endoderm fate which serves as a pathway towards more functional cell types like the pancreatic cells. Research over the past decade has established several robust pathways for deriving endoderm from hESCs, with the capability of further maturation. However, in our experience, the functional maturity of these endoderm derivatives, specifically to pancreatic lineage, largely depends on specific pathway of endoderm induction. Hence it will be of interest to understand the underlying mechanism mediating such induction and how it is translated to further maturation. In this work we analyze the regulatory interactions mediating different pathways of endoderm induction by identifying co-regulated transcription factors.

Results

hESCs were induced towards endoderm using activin A and 4 different growth factors (FGF2 (F), BMP4 (B), PI3KI (P), and WNT3A (W)) and their combinations thereof, resulting in 15 total experimental conditions. At the end of differentiation each condition was analyzed by qRT-PCR for 12 relevant endoderm related transcription factors (TFs). As a first approach, we used hierarchical clustering to identify which growth factor combinations favor up-regulation of different genes. In the next step we identified sets of co-regulated transcription factors using a biclustering algorithm. The high variability of experimental data was addressed by integrating the biclustering formulation with bootstrap re-sampling to identify robust networks of co-regulated transcription factors. Our results show that the transition from early to late endoderm is favored by FGF2 as well as WNT3A treatments under high activin. However, induction of late endoderm markers is relatively favored by WNT3A under high activin.

Conclusions

Use of FGF2, WNT3A or PI3K inhibition with high activin A may serve well in definitive endoderm induction followed by WNT3A specific signaling to direct the definitive endoderm into late endodermal lineages. Other combinations, though still feasible for endoderm induction, appear less promising for pancreatic endoderm specification in our experiments.
Sex-specific signaling through Toll-Like Receptors 2 and 4 contributes to survival outcome of Coxsackievirus B3 infection in C57Bl/6 mice
Authors: Roberts Brian J , Dragon Julie A , Moussawi Mohamad , Huber Sally A
Published: December 2012

Background

Coxsackievirus B3 (CVB3) induces myocarditis, an inflammatory heart disease, which affects men more than women. Toll-like receptor (TLR) signaling has been shown to determine the severity of CVB3-induced myocarditis. No direct role for signaling through TLR2 had been shown in myocarditis although published studies show that cardiac myosin is an endogenous TLR2 ligand and stimulates pro-inflammatory cytokine expression by dendritic cellsin vitro. The goal of this study is to determine which TLRs show differential expression in CVB3 infected mice corresponding to male susceptibility and female resistance in this disease.

Methods

Male and female C57Bl/6 mice were infected with 102PFU CVB3 and killed on day 3 or 6 post infection. Hearts were evaluated for virus titer, myocardial inflammation, and TLR mRNA expression by PCR array and microarray analysis. Splenic lymphocytes only were evaluated by flow cytometry for the number of TLR+/CD3+, TLR+/CD4+, TLR+F4/80+ and TLR+/CD11c+ subpopulations and the mean fluorescence intensity to assess upregulation of TLR expression on these cells. Mice were additionally treated with PAM3CSK4 (TLR2 agonist) or ultrapure LPS (TLR4 agonist) on the same day as CVB3 infection or 3 days post infection to confirm their role in myocarditis susceptibility.

Results

Despite equivalent viral titers, male C57Bl/6 mice develop more severe myocarditis than females by day 6 after infection. Microarray analysis shows a differential expression of TLR2 at day 3 with female mice having higher levels of TLR2 gene expression compared to males. Disease severity correlates to greater TLR4 protein expression on splenic lymphocytes in male mice 3 days after infection while resistance in females correlates to preferential TLR2 expression, especially in spleen lymphocytes. Treating male mice with PAM reduced mortality from 55% in control CVB3 infected animals to 10%. Treating female mice with LPS increased mortality from 0% in control infected animals to 60%.

Conclusion

CVB3 infection causes an up-regulation of TLR2 in female and of TLR4 in male mice and this differential expression between the sexes contributes to disease resistance of females and susceptibility of males. While previous reports demonstrated a pathogenic role for TLR4 this is the first report that TLR2 is preferentially up-regulated in CVB3 infected female mice or that signaling through this TLR directly causes myocarditis resistance.