bluuebunny/crossref_metadata_2025 huggingface dataset:
An Effective Decoupling Control with Simple Structure for Induction Motor Drive System Considering Digital Delay
Authors: Wang Cheng , Jaidaa Asem , Wang Ze , Lu Lei
Published: December 2021

Digital processing poses a considerable time delay on controllers of induction motor (IM) driving system, which degrades the effects of torque/flux decoupling, slows the motor torque response down, or even makes the entire system unstable, especially when operating at a low switching frequency. The existing methods, such as feed-forward and feed-back decoupling methods based on the proportional integral controller (PI), have an intrinsic disadvantage in the compromise between high performance and low switching frequency. Besides, the digital delay cannot be well compensated, which may affect the system loop and bring instability. Conventional complex vector decoupling control based on an accurate IM model employs complicated decoupling loops that may be degraded by digital delay leading to discrete error. This article aims to give an alternative complex vector decoupling solution with a simple structure, intended for optimized decoupling and improving the system dynamic performance throughout the entire operating range. The digital delay-caused impacts, including secondary coupling effect and voltage vector amplitude/phase inaccuracy, are specified. Given this, the digital delay impact is canceled accurately in advance, simplifying the entire decoupling process greatly while achieving uncompromised decoupling performance. The simulation and experimental results prove the effectiveness and feasibility of the proposed decoupling technique.
A Traffic-Aware Federated Imitation Learning Framework for Motion Control at Unsignalized Intersections with Internet of Vehicles
Authors: Wu Tianhao , Jiang Mingzhi , Han Yinhui , Yuan Zheng , Li Xinhang , Zhang Lin
Published: December 2021

The wealth of data and the enhanced computation capabilities of Internet of Vehicles (IoV) enable the optimized motion control of vehicles passing through an intersection without traffic lights. However, more intersections and demands for privacy protection pose new challenges to motion control optimization. Federated Learning (FL) can protect privacy via model interaction in IoV, but traditional FL methods hardly deal with the transportation issue. To address the aforementioned issue, this study proposes a Traffic-Aware Federated Imitation learning framework for Motion Control (TAFI-MC), consisting of Vehicle Interactors (VIs), Edge Trainers (ETs), and a Cloud Aggregator (CA). An Imitation Learning (IL) algorithm is integrated into TAFI-MC to improve motion control. Furthermore, a loss-aware experience selection strategy is explored to reduce communication overhead between ETs and VIs. The experimental results show that the proposed TAFI-MC outperforms imitated rules in the respect of collision avoidance and driving comfort, and the experience selection strategy can reduce communication overheads while ensuring convergence.
Modeling the Potential Distribution of Three Taxa of Akebia Decne. under Climate Change Scenarios in China
Authors: Wang Xiuting , Zhang Wenwen , Zhao Xin , Zhu Huiqin , Ma Limiao , Qian Zengqiang , Zhang Zheng
Published: December 2021

Akebia trifoliata (Thunb.) Koidz., Akebia trifoliata subsp. australis (Diels) T. Shimizu and Akebia quinata (Houtt.) Decne. are the source plants of the traditional Chinese medicines AKEBIAE CAULIS and AKEBIAE FRUCTUS, and have high pharmaceutical value. However, the resource reserve of these plants has dramatically declined due to habitat destruction, which has seriously affected their adequate supply and sustainable utilization. A poor knowledge of the potential distribution of these medicinal materials would seriously constrain the protective exploitation of wild resources and the establishment of new cultivations. In this study, based on the scenarios of SSP1-2.6, SSP2-4.5, SSP3-7.0 and SSP5-8.5, the maximum entropy model was used to predict the potential distribution of these three Akebia taxa under current and future (2030s, 2050s, 2070s and 2090s) climate conditions. Our findings showed that the potentially suitable areas of these three Akebia taxa were mainly distributed in China at 101.8–121.9° E and 23.5–34.6° N. Temperature played a more significant role than precipitation in affecting the distribution. The dominant bioclimatic variable that affected the distribution of A. trifoliata and A. quinata in China was the minimum temperature of the coldest month (BIO06). For A. trifoliata subsp. australis, the mean diurnal range (BIO02) was the dominant variable influencing its distribution. Compared with current conditions, the moderate- and high-suitability areas of these three Akebia taxa were predicted to shrink towards the core areas, while the low-suitability areas were all observed to increase from the 2030s to the 2090s. With the increase in radiative forcing of SSP, the low-impact areas of these three Akebia taxa showed a decreasing trend as a whole. Our results illustrate the impact of climate change on the distribution of Akebia, and would provide references for the sustainable utilization of Akebia’s resources.
Tree Morphometric Relationships and Dynamic Elasticity Properties in Tropical Rain Tree (Samanea saman Jacq. Merr)
Authors: Karlinasari Lina , Adzkia Ulfa , Puspitasari Tiara , Nandika Dodi , Nugroho Naresworo , Syafitri Utami Dyah , Siregar Iskandar Z.
Published: December 2021

Rain tree (Samanea saman) is a dominant species commonly planted as a shade tree in urban landscapes in the tropics, including in Indonesia. This species is also targeted for routine inspection and monitoring within tree management systems. Management should particularly focus on tree health through assessment of tree growth conditions as well as tree stability. However, baseline information on the relationships of many key characteristics for the rain tree (e.g., growth, morphometrics, and biomechanics) is lacking. Therefore, an investigation was carried out to determine the relationship of morphometric parameters to tree growth, which included use of an acoustic tool to assess the elasticity of the tree stem. Fifty rain trees were selected for data collection and evaluated using stress wave velocity tools and visual morphometrics assessment. The results demonstrated that, in general, the trees had good vigor and stability. Statistical analysis showed a strong relationship between tree growth parameters and selected morphometric characteristics. The use of a nondestructive acoustic-based instrument enabled determining the modulus of elasticity, which provided equivalent results with the elasticity value obtained through a common method for determining tree stability. Only live crown ratio and tree slenderness parameters had a relationship with the dynamic modulus of elasticity. These results offer a means of measurement that is simpler, less expensive, and faster than current methods, although further testing is needed for different tree species and growth sites.
Contrasts in Top Soil Infiltration Processes for Degraded vs. Restored Lands. A Case Study at the Perijá Range in Colombia
Authors: Lozano-Baez Sergio Esteban , Domínguez-Haydar Yamileth , Zwartendijk Bob W. , Cooper Miguel , Tobón Conrado , Di Prima Simone
Published: December 2021

Governments are increasingly committing to significant ecological restoration. However, the impacts of forest restoration on local hydrological services are surprisingly poorly understood. Particularly, limited information is available about the impacts of tree planting on soil infiltration processes and runoff pathways. Thus, we investigated the saturated hydraulic conductivity (Ks) and preferential flow pathways in three land-cover types: (i) Active Restoration, (ii) Degraded Land, and (iii) Reference Forest, with contrasting differences in soil profile and land use history in the municipality of La Jagua de Ibirico, César department, Colombia. We conducted soil sampling, using the Beerkan method to determine Ks values. We also measured vegetation attributes (i.e., canopy cover, vegetation height, diameter at breast height, and total number of trees) and carried out three dye tracer experiments for each study site. The blue dye experiments revealed that near surface matrix infiltration was dominant for Degraded Land, while at the Active Restoration and Reference Forest, this only occurred at local surface depressions. The general infiltration pattern at the three land uses is indicated as being macropore flow with mixed interaction with the matrix and highly affected by the presence of rock fragments. The deeper infiltration patterns occur by preferential flow due to the presence of roots and rock fragments. The mean Ks for the Active Restoration (240 mm h−1) was much higher than the Ks at Degraded Land (40 mm h−1) but still considerably lower than the Reference Forest (324 mm h−1). These results indicate that top soil infiltration capacity and soil physical parameters not only directly regulate the amount of infiltration but also infiltration patterns and runoff processes, leading to lower infiltration and increased excess overland flow for Degraded Land than for other land uses.
Potential Link between Ectomycorrhizal Community Composition and Host Tree Phenology
Authors: Unuk Nahberger Tina , Damjanič Rok , Kraigher Hojka , Grebenc Tine
Published: December 2021

The timing of leaf phenology tends to be crucial in controlling ecosystem processes such as the acquisition of carbon and water loss as well as in controlling tree nutrient cycling. To date, tree phenology has mostly been associated with environmental control (e.g., temperature and photoperiod) in a relationship with inheritance, while it has rarely been linked with ectomycorrhizal community compositional changes through the host tree’s phenological stages. Seasonal variations of fungal communities have been widely studied, but little is known about mycorrhiza community composition changes along phenological stages. Therefore, we analyzed ectomycorrhizal communities associated with silver fir and their compositional changes during the transition between phenological stages. The phenological stages of each individual tree and time of bud break were monitored weekly for two years and, at the same time, ectomycorrhiza was harvested from selected silver fir trees. In total, 60 soil cores were analyzed for differences in the ectomycorrhizal community between phenological stages using Sanger sequencing of individual ectomycorrhizal morphotypes. A significant difference in beta diversity for an overall ectomycorrhizal community was confirmed between analyzed time periods for both sampled years. Species-specific reactions to transitions between phenological stages were confirmed for 18 different ectomycorrhizal taxa, where a positive correlation of Russula ochroleuca, Russula illota, Tomentella sublilacina, and Tylospora fibrillosa was observed with the phenological stage of bud burst.
Logging Industry in the United States: Employment and Profitability
Authors: He Mingtao , Smidt Mathew , Li Wenying , Zhang Yaoqi
Published: December 2021

This study analyzed logging industry employment and profitability in recent decades in the U.S. based on Occupational Employment Statistics (OES), Quarterly Workforce Indicators (QWI), Quarterly Census of Employment and Wages (QCEW), and Timber Product Output (TPO) Reports. The logging industry in the U.S. has experienced reduced employment with an aging workforce over the past two decades. The changes might be related to increased productivity from mechanization, combined with reduced demand for logging, but estimates of capital and labor productivity for logging are not available. To overcome the data limitation, a simple and cost-effective economic model, Economic Input-Output Life Cycle Assessment (EIO-LCA) Model, was applied to estimate the profitability of the entire industry at a state level. It was found that the reduced demand and increased operating costs led to poor profitability and a wave of closures of logging firms but also accelerated the adjustment in the logging industry. Serious challenges facing the forestry sector include the lack of an effective monitoring tool for the logging industry, structural shortage of logging labor, and rising operating costs.
Effect of Perforation Dyeing Technique on Color Difference, Colorfastness, and Basic Density of Living Red-Heart Chinese Fir
Authors: Wang Yiying , Qu Ruru , Deng Xiangwen , Huang Zhihong , Xiang Wenhua , Ouyang Shuai
Published: December 2021

Red-heart Chinese fir is an excellent geographic provenance of Cunninghamia lanceolata, with high-value red heartwood. However, the formation of red heartwood is usually slow. To quickly cultivate red-heart Chinese fir, we studied perforation dyeing technology on living trees that were 7 years old and efficient in high-value red heartwood formation. Reactive dye (%), penetrant (%), KH2PO4 (%), and pH were selected as influencing factors, and an orthogonal test (L9(3)4) was used. The results showed that the total color difference between the experimental and CK groups ranged from 13.74 to 26.86 NBS, which was a significant visual perception (above 12 NBS). The total color difference before and after soaking in water for 6 h ranged from 2.30 to 5.12 NBS, which belonged to the detectable and identifiable value of the human eye (2~5 NBS). After the injection of the dye liquid, the wood basic density (WBD) was significantly affected after one year. After a comprehensive analysis of wood color difference, colorfastness, and WBD of the orthogonal test, the best dyeing process of juvenile red-heart Chinese fir was reactive dye: 0.8%, penetrant: 0.05%, KH2PO4: 0.3%, and pH: 3.5. The results of this study can provide a reference to improve the value of red-heart Chinese fir, a fast-cultivated, high-value decorative wood material.
Methodology for Assessing and Managing the Environmental Performance of Skidding and Feller Buncher Tractors
Authors: Grigorev Igor , Kunickaya Ol’ga , Tikhonov Evgeniy , Hertz Edward , Khakhina Anna , Burmistrova Olga , Sukhomlinova Natalia , Zhuk Artem
Published: December 2021

Systematic assessments on the effects of skidding systems on features of forest blueberry pine soil were conducted as part of this study. Assessing the ecological efficiency of forest skidding machines showed that the most significant impact (by 2.0–2.2 times) on soil compaction was observed at loading sites rather than during transportation. Lightweight loam density and sand density increased by 25% and 2%, respectively, after more than two passages of the skidding system. Pressure in 33L-32 tires of forestry machinery in operation on a solid surface varied from 46.5 kPa to 196 kPa at maximum load. Studying the impact of tires on soil compaction showed that the environmental efficiency of forestry equipment can be enhanced if the optimal tire pressure at average loads does not exceed 70 kPa for tracked vehicles and 150 kPa for wheeled vehicles in summer seasons. When ground grips were fully immersed, the pressure of forwarders on soil was reduced. These study results can be used to establish organizational and technological measures in order to manage the negative impact of skidding systems and to increase the environmental effects of their performance.
Bioconversion of Ginsenosides in American Ginseng Extraction Residue by Fermentation with Ganoderma lucidum Improves Insulin-like Glucose Uptake in 3T3-L1 Adipocytes
Authors: Hsu Bo-Yang , Chen Chia-Hui , Lu Ting-Jang , Pan Min-Hsiung , Ho Chi-Tang , Hwang Lucy Sun , Hung Wei-Lun
Published: December 2021

Ginseng is one of the most popular traditional Chinese medicines that have been widely used in China and other Asian countries for thousands of years. Ginsenosides are the unique bioactive saponins occurring in ginseng, and their biological activities have been extensively investigated. A large amount of ginseng residue is produced as waste product due to its applications in manufacturing functional food products, even though it may still contain bioactive components. Thus, the objective of this study was to investigate the hypoglycemic activities of American ginseng extraction residue (AmR) via fermentation with Ganoderma lucidum. Our results showed that the total phenolic contents and β-glucosidase activity of AmR profoundly increased after fermentation with G. lucidum. In 3T3-L1 adipocytes, stimulation of glucose uptake by treatment with AmR was not significant, while fermented AmR (FAmR) exhibited insulin-like glucose-uptake-stimulatory effects. Importantly, the hypoglycemic effects of FAmR were positively associated with the amount of the deglycosylated minor ginsenosides Rg1, Rg3, and compound K. Taken together, our current findings suggest that bioconversion of AmR by fermentation with G. lucidum may be a feasible and eco-friendly approach to developing a functional ingredient for the management of diabetes, while also resolving the problem of ginseng waste.
Securing Environmental IoT Data Using Masked Authentication Messaging Protocol in a DAG-Based Blockchain: IOTA Tangle
Authors: Gangwani Pranav , Perez-Pons Alexander , Bhardwaj Tushar , Upadhyay Himanshu , Joshi Santosh , Lagos Leonel
Published: December 2021

The demand for the digital monitoring of environmental ecosystems is high and growing rapidly as a means of protecting the public and managing the environment. However, before data, algorithms, and models can be mobilized at scale, there are considerable concerns associated with privacy and security that can negatively affect the adoption of technology within this domain. In this paper, we propose the advancement of electronic environmental monitoring through the capability provided by the blockchain. The blockchain’s use of a distributed ledger as its underlying infrastructure is an attractive approach to counter these privacy and security issues, although its performance and ability to manage sensor data must be assessed. We focus on a new distributed ledger technology for the IoT, called IOTA, that is based on a directed acyclic graph. IOTA overcomes the current limitations of the blockchain and offers a data communication protocol called masked authenticated messaging for secure data sharing among Internet of Things (IoT) devices. We show how the application layer employing the data communication protocol, MAM, can support the secure transmission, storage, and retrieval of encrypted environmental sensor data by using an immutable distributed ledger such as that shown in IOTA. Finally, we evaluate, compare, and analyze the performance of the MAM protocol against a non-protocol approach.
Hidden Giants: The Story of Bolbometopon muricatum at Ningaloo Reef
Authors: Thomson Damian P. , Cresswell Anna K. , Doropoulos Christopher , Haywood Michael D. E. , Orr Melanie , Hoey Andrew S.
Published: December 2021

Bolbometopon muricatum (bumphead parrotfish, Valenciennes, 1839) is a conspicuous, iconic and ecologically important coral reef fish species. B. muricatum plays an important role in the bioerosion of the reef framework and as a result has been described as both an ecosystem engineer and keystone species. Despite the complete absence of B. muricatum from 32 years of scientific surveys across the Ningaloo Reef World Heritage Area, we recorded a total of 155 individuals of B. muricatum across 63.2 ha of reef crest surveys, equating to mean density of 2.38 ind/ha. Our observations represent the first record of this iconic species in scientific surveys at Ningaloo and in combination with qualitative observations of B. muricatum by expert witnesses, indicate B. muricatum is likely to have been present in ecologically relevant densities since 2006. The densities of B. muricatum observed at northern Ningaloo in 2021 suggest this species is removing an estimated 13.42 tonnes/ha or 1.34 kg/m2 of calcium carbonate per year, which is broadly comparable with estimates of total parrotfish bioerosion across many reefs in the central Indian and Pacific Oceans. Although not currently afforded elevated conservation status within management plans, B. muricatum possess many life-history characteristics that make them vulnerable to overfishing and may justify consideration for increased protection within the world heritage listed Ningaloo Reef Marine Park.
Design and Fabrication of an Automatic Fish Feeder Prototype Suits Tilapia Tanks
Authors: El Shal Ahmed Mohamed , El Sheikh Faisal Mohamed , Elsbaay Atef Mohamed
Published: December 2021

The conventional methods of supplying feed to tilapia tanks are ineffective. It is better to find new a automatic feeder saving pellets from crushing and cohesion without hitting pellets during feeding at a predetermined interval of time and an accurate amount of food with a larger surface area covered by pellets. Developing-country fish farmers use manual feeding to be more cost-effective than with costly mechanized feeding, so this research aimed to design and construct an automatic fish feeder prototype to feed tilapia in a recirculation aquaculture system’s tank. The performance of the prototype was studied after it was designed and installed. The dispensed feed operated by a DC motor located underneath the pellet hopper and the feed material was discharged into the tank through a gate in the bottom of the feeder. Three pellet sizes, three rotation speeds, and three feeder heights from the water’s surface were used to test the automatic feeder’s efficiency. The results showed that the optimal speed for the automatic feeder was 14 rpm with a height of 70 cm, resulting in a distribution width of 26.6 cm and a high automatic feeder efficiency of 99.9%. Furthermore, the feeder used very little electricity and saved time, cost, labor, energy, and pellets.
Influence of Food Matrices and the Population of Interfering Microorganisms on the Determination of Listeria monocytogenes by Conventional Methods and VIDAS
Authors: Estévez Maribel , García-Viejo Fernando , López-Mendoza Mª Carmen , Jordano Rafael , Medina Luis M.
Published: December 2021

In this study, the possible influence of the food matrix and the interfering population of microorganisms on the detection and count of Listeria monocytogenes in three common foods of the Spanish diet (Spanish omelette, fresh cheese and vegetable salad) was determined. Four groups were assayed: one control, two groups with interfering microorganisms (Salmonella Enteritidis, Staphylococcus aureus and Proteus mirabilis) with different levels of L. monocytogenes and a final group only contaminated with L. monocytogenes. The samples were analyzed with the normalized method (UNE-EN ISO 11290:2018) and with an alternative technique (VIDAS). The results show that the presence of interfering microorganisms did not seem to interfere with the determination of L. monocytogenes. Furthermore, the type of food did not seem to influence the determination of L. monocytogenes, but the culture media used showed differences. In fact, regardless of the type of food, the ALOA medium showed higher sensitivity than the other media, with higher recovery in 100% of samples (only for the Spanish omelette in Group B was the result the same as that for PALCAM, −8.11 log cfu/g). The results obtained using the VIDAS were not influenced by any of the factors or conditions used and show 100% efficiency.
The Regulatory Effects of Citrus Peel Powder on Liver Metabolites and Gut Flora in Mice with Non-Alcoholic Fatty Liver Disease (NAFLD)
Authors: Hu Meiyi , Zhang Li , Ruan Zheng , Han Peiheng , Yu Yujuan
Published: December 2021

Gannan navel orange and Jinggang pomelo, belonging to the genus Citrus, are good sources of phenolic compounds, which are mainly concentrated in the peel. These phenolic compounds are considered promising in the prevention and treatment of non-alcoholic fatty liver disease (NAFLD). In order to maximize nutrients retention and bioactivity in the peel, pomelo peel and orange peel were processed using freeze-drying technology and mixed in the ratio (pomelo peel powder 50% and orange peel powder 50%) to make citrus peel powder (CPP). The purpose of this study was to explore new strategies and mechanisms associated with the consumption of CPP to alleviate nonalcoholic fatty liver injury, lipid metabolism disorders, and gut microbiota dysbiosis in obese mice induced by high-fat diet (HFD). The results showed that after 12 weeks of CPP administration, CPP supplementation had a strong inhibitory effect on HFD-induced weight gain, hepatic fat accumulation, dyslipidemia, and the release of pro-inflammatory cytokines. In particular, CPP modulates the composition of the intestinal flora, such as increasing the relative abundance of phylum Firmicutes, genus Faecalibaculum, genus Lactobacillus, genus Dubosiella, and genus Lachnospiraceae_NK4A136_ group and decreasing the relative abundance of phylum Bacteroidota, genus Helicobacter, and genus Bacteroides. These results suggest that CPP has a preventive effect on NAFLD, which can be related to the regulation of intestinal flora.
Lubrication and Sensory Properties of Emulsion Systems and Effects of Droplet Size Distribution
Authors: Wang Qi , Zhu Yang , Ji Zhichao , Chen Jianshe
Published: December 2021

The functional and sensory properties of food emulsion are thought to be complicated and influenced by many factors, such as the emulsifier, oil/fat mass fraction, and size of oil/fat droplets. In addition, the perceived texture of food emulsion during oral processing is mainly dominated by its rheological and tribological responses. This study investigated the effect of droplet size distribution as well as the content of oil droplets on the lubrication and sensory properties of o/w emulsion systems. Friction curves for reconstituted milk samples (composition: skimmed milk and milk cream) and Casein sodium salt (hereinafter referred to as CSS) stabilized model emulsions (olive oil as oil phase) were obtained using a soft texture analyzer tribometer with a three ball-on-disc setup combined with a soft surfaces (PDMS) tribology system. Sensory discrimination was conducted by 22 participants using an intensity scoring method. Stribeck curve analyses showed that, for reconstituted milk samples with similar rheological properties, increasing the volume fraction of oil/fat droplets in the size range of 1–10 µm will significantly enhance lubrication, while for CSS-stabilized emulsions, the size effect of oil/fat droplets reduced to around 1 µm. Surprisingly, once the size of oil/fat droplets of both systems reached nano size (d90 = 0.3 µm), increasing the oil/fat content gave no further enhancement, and the friction coefficient showed no significant difference (p > 0.05). Results from sensory analysis show that consumers are capable of discriminating emulsions, which vary in oil/fat droplet size and in oil/fat content (p < 0.01). However, it appeared that the discrimination capability of the panelist was significantly reduced for emulsions containing nano-sized droplets.
Comparative Peptidomics Analysis of Fermented Milk by Lactobacillus delbrueckii ssp. bulgaricus and Lactobacillus delbrueckii ssp. lactis
Authors: Ye Hongji , Zhang Xinyi , Jiang Yang , Guo Min , Liu Xiaoming , Zhao Jianxin , Zhang Hao , Chen Wei
Published: December 2021

Few studies have investigated the peptidomics of fermented milk by Lactobacillus delbrueckii. The aim of the present study was to interpret the peptidomic pattern of the fermented milk by five strains of L. delbrueckii ssp. bulgaricus and ssp. lactis prior to and after the simulated gastrointestinal digestion in vitro. The results indicated variations in the peptidomics among the samples, particularly between the samples of different subspecies. The peptides originating from β-casein were abundant in the samples of ssp. bulgaricus, whereas the peptides derived from αs1-casein and αs2-casein were more likely to dominate in those of ssp. lactis. For β-casein, the strains of ssp. bulgaricus displayed extensive hydrolysis in the regions of (73–97), (100–120), and (130–209), whereas ssp. lactis mainly focused on (160–209). The digestion appears to reduce the variations of the peptidomics profile in general. Among the five strains, L. delbrueckii ssp. bulgaricus DQHXNS8L6 was the most efficient in the generation of bioactive peptides prior to and after digestion. This research provided an approach for evaluating the peptide profile of the strains during fermentation and digestion.
Antioxidative Responses to Pre-Storage Hot Water Treatment of Red Sweet Pepper (Capsicum annuum L.) Fruit during Cold Storage
Authors: Kantakhoo Jirarat , Imahori Yoshihiro
Published: December 2021

The effects of hot water treatments on antioxidant responses in red sweet pepper (Capsicum annuum L.) fruit during cold storage were investigated. Red sweet pepper fruits were treated with hot water at 55 °C for 1 (HWT-1 min), 3 (HWT-3 min), and 5 min (HWT-5 min) and stored at 10 °C for 4 weeks. The results indicated that HWT-1 min fruit showed less development of chilling injury (CI), electrolyte leakage, and weight loss. Excessive hot water treatment (3 and 5 min) caused cellular damage. Moreover, HWT-1 min slowed the production of hydrogen peroxide and malondialdehyde and promoted the ascorbate and glutathione contents for the duration of cold storage as compared to HWT-3 min, HWT-5 min, and control. HWT-1 min enhanced the ascorbate-glutathione cycle associated with ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase, but it was less effective in simulating catalase activity. Thus, HWT-1 min could induce CI tolerance in red sweet pepper fruit by activating the ascorbate-glutathione cycle via the increased activity of related enzymes and the enhanced antioxidant level.
A Comparative Functional Analysis of Pea Protein and Grass Carp Protein Mixture via Blending and Co-Precipitation
Authors: Zhou Xiaohu , Zhang Chaohua , Cao Wenhong , Zhou Chunxia , Zheng Huina , Zhao Liangzhong
Published: December 2021

Currently, the application of protein mixture derived from plants and animals is of great interest to the food industry. However, the synergistic effects of isolated protein blends (BL) are not well established. Herein, the development of a more effective method (co-precipitation) for the production of protein mixtures from pea and grass carp is reported. Pea protein isolate (PPI), grass carp protein isolate (CPI), and pea–carp protein co-precipitates (Co) were prepared via isoelectric solubilization/precipitation using peas and grass carp as raw materials. Meanwhile, the BL was obtained by blending PPI with CPI. In addition, the subunit composition and functional properties of Co and BL were investigated. The results show that the ratios of vicilin to legumin α + β and the soluble aggregates of Co were 2.82- and 1.69-fold higher than that of BL. The surface hydrophobicity of Co was less than that of BL, PPI, and CPI (p < 0.05). The solubility of Co was greater than that of BL, PPI, and CPI (p < 0.05), and the foaming activity was higher than that of BL and CPI (p < 0.05) but slightly lower than that of PPI. In addition, based on the emulsifying activity index, particle size, microstructure, and viscosity, Co had better emulsifying properties than BL, PPI, and CPI. The study not only confirmed that co-precipitation was more effective than blending for the preparation of mixed protein using PPI and CPI but also provided a standard of reference for obtaining a mixture of plant and animal proteins.
Distribution and Quantification of 1,2-Propylene Glycol Enantiomers in Baijiu
Authors: Xu Hao , Dai Yifeng , Qiu Shuyi , Sun Baoguo , Zeng Xiangyong
Published: December 2021

Enantiomers of 1,2-Propylene glycol (1,2-PG) were investigated in 64 commercial Chinese Baijiu including soy sauce aroma-type Baijiu (SSB), strong aroma-type Baijiu (STB), and light aroma-type Baijiu (LTB), via chiral gas chromatography (β-cyclodextrin). The natural enantiomeric distribution and concentration of 1,2-PG in various baijiu were studied to evaluate whether the distribution and content of the two isomers of 1,2-PG were correlated with the aroma type and storage year. The results showed that 1,2-PG has a high enantiomeric ratio and the (S)-configuration predominated in SSB. The average S/R enantiomeric ratio of this compound in SSB was approximately 87:13 (±3.17), with an average concentration of 52.77 (±23.70) mg/L for the (S)-configuration and 8.72 (±3.63) mg/L for the (R)-enantiomer. The (R)-configuration was predominant in the STB, whereas neither (S) nor (R)-form of 1,2-PG were detected in LTB. The content of the two configurations of 1,2-PG in the JSHSJ vintage of SSB showed a wave variation, with an average S/R enantiomeric ratio of 89:11 (±1.15). The concentration of (R)-1,2-PG in XJCTJ vintage liquors showed an upward and then downward trend with aging time, with an overall downward trend, and the concentration of (S)-form showed a wavy change with an overall upward trend. Except for the LZLJ-2019 vintage where both (R) and (S)-1,2-PG were present, all other samples only existed (R)-form, and a decreasing trend of (R)-enantiomer with aging time was observed. The enantiomeric ratio of 1,2-PG might be one of the potential markers for adulteration control of Baijiu as industrial 1,2-PG usually presented in the racemic mixture. Sensory analysis revealed olfactory thresholds of 4.66 mg/L and 23.92 mg/L for the (R)- and (S)-configurations in pure water respectively. GC-O showed both enantiomers exhibited different aromatic nuances.
The Investigation of Mycotoxins and Enterobacteriaceae of Cereal-Based Baby Foods Marketed in Turkey
Authors: Er Demirhan Buket , Demirhan Burak
Published: December 2021

In this study, a total of 85 cereal-based baby foods with or without milk (four different brands; A, B, C, and D) collected from Ankara local markets, Turkey were analyzed for mycotoxins, total aerobic mesophilic bacteria (TAMB), and Enterobacteriaceae contamination. Baby foods were analyzed for 12 toxicological important mycotoxins such as aflatoxin B1, B2, G1, and G2; fumonisin B1 and B2; ochratoxin A; sterigmatocystin (STE); deoxynivalenol (DON); zearalenone (ZON); and T-2 toxin and HT-2 toxin by LC-MS/MS multi-mycotoxin method. In addition to these mycotoxins, the presence of aflatoxin M1 (AFM1) was investigated in baby foods containing milk. The classical culture method was used for microbiological analysis. Consequently, at least one mycotoxin was detected in 69.41% of the total samples. The most frequently detected mycotoxins were STE (34.12%) and HT-2 (34.12%). However, AFM1 was not detected in any of the baby foods containing milk. Also, TAMB and Enterobacteriaceae were isolated from 30.59% and 10.59% of samples, respectively. As a result, it was determined that the mycotoxin levels in the analyzed samples were in accordance with the mycotoxin levels specified in the Turkish Food Codex.
LC-QTOF-MS/MS Based Molecular Networking Approach for the Isolation of α-Glucosidase Inhibitors and Virucidal Agents from Coccinia grandis (L.) Voigt
Authors: Astiti Maharani A. , Jittmittraphap Akanitt , Leaungwutiwong Pornsawan , Chutiwitoonchai Nopporn , Pripdeevech Patcharee , Mahidol Chulabhorn , Ruchirawat Somsak , Kittakoop Prasat
Published: December 2021

Coccinia grandis or ivy gourd is an edible plant. Its leaves and fruits are used as vegetable in many countries. Many works on antidiabetic activity of a crude extract of C. grandis, i.e., in vitro, in vivo, and clinical trials studies, have been reported. Profiles of the antidiabetic compounds were previously proposed by using LC-MS or GC-MS. However, the compounds responsible for antidiabetic activity have rarely been isolated and characterized by analysis of 1D and 2D NMR data. In the present work, UHPLC-ESI-QTOF-MS/MS analysis and GNPS molecular networking were used to guide the isolation of α-glucosidase inhibitors from an extract of C. grandis leaves. Seven flavonoid glycosides including rutin (1), kaempferol 3-O-rutinoside (2) or nicotiflorin, kaempferol 3-O-robinobioside (3), quercetin 3-O-robinobioside (4), quercetin 3-O-β-D-apiofuranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→6)]-β-D-glucopyranoside (5) or CTN-986, kaempferol 3-O-β-D-api-furanosyl-(1→2)-[α-L-rhamnopyranosyl-(1→6)]-β-D-glucopyranoside (6), and kaempferol 3-O-β-D-apiofuranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→6)]-β-D-galactopyranoside (7) were isolated from C. grandis leaves. This is the first report of glycosides containing apiose sugar in the genus Coccinia. These glycosides exhibited remarkable α-glucosidase inhibitory activity, being 4.4–10.3 times more potent than acarbose. Moreover, they also displayed virucidal activity against influenza A virus H1N1, as revealed by the ASTM E1053-20 method.
Nonexistence of Global Solutions to Time-Fractional Damped Wave Inequalities in Bounded Domains with a Singular Potential on the Boundary
Authors: Bin Sultan Areej , Jleli Mohamed , Samet Bessem
Published: December 2021

We first consider the damped wave inequality ∂2u∂t2−∂2u∂x2+∂u∂t≥xσ|u|p,t>0,x∈(0,L), where L>0, σ∈R, and p>1, under the Dirichlet boundary conditions (u(t,0),u(t,L))=(f(t),g(t)),t>0. We establish sufficient conditions depending on σ, p, the initial conditions, and the boundary conditions, under which the considered problem admits no global solution. Two cases of boundary conditions are investigated: g≡0 and g(t)=tγ, γ>−1. Next, we extend our study to the time-fractional analogue of the above problem, namely, the time-fractional damped wave inequality ∂αu∂tα−∂2u∂x2+∂βu∂tβ≥xσ|u|p,t>0,x∈(0,L), where α∈(1,2), β∈(0,1), and ∂τ∂tτ is the time-Caputo fractional derivative of order τ, τ∈{α,β}. Our approach is based on the test function method. Namely, a judicious choice of test functions is made, taking in consideration the boundedness of the domain and the boundary conditions. Comparing with previous existing results in the literature, our results hold without assuming that the initial values are large with respect to a certain norm.
The Nature of Micro-Variability in Blazars
Authors: Webb James R. , Arroyave Viviana , Laurence Douglas , Revesz Stephen , Bhatta Gopal , Hollingsworth Hal , Dhalla Sarah , Howard Emily , Cioffi Michael
Published: December 2021

We present the results of a long-term study designed to investigate the nature of micro-variability in blazars carried out primarily at the Southeastern Association for Research in Astronomy (SARA) observatories. We analyzed micro-variability data of fifteen OVV quasars and BL Lac sources collected from 1995 to 2021. The data set consists of single-band light curves interspersed with multi-color and micro-variability observations. This paper reports over 900 nights of CCD observations. We also incorporated observations from other observers as well as observations gleaned from the literature into our analysis. We employed differential photometry to measure magnitudes and then construct the long-term and micro-variability light curves. Our results indicate that there is no correlation between the presence of micro-variations and the brightness of the source. We present a viable theory to explain the intermittent micro-variability as pulses of radiation emitted by individual turbulent cells in the relativistic jet, which are stimulated by a passing shock wave. We present model fits and test results for various data sets, including WEBT light curves, Kepler light curves and a TESS light curve. Although the consensus in the community is that blazar jets must be turbulent, the identification of micro-variations as manifestations of actual turbulent cells is important for modeling these turbulent jets. We can obtain estimates of cell sizes (assuming a shock speed), and the distribution of cell sizes derived from observations is consistent with numerical simulation predictions.
Preparation and Characterization of Nanocellulose/Chitosan Aerogel Scaffolds Using Chemical-Free Approach
Authors: Rizal Samsul , Yahya Esam Bashir , Abdul Khalil H. P. S. , Abdullah C. K. , Marwan Marwan , Ikramullah Ikramullah , Muksin Umar
Published: December 2021

Biopolymer-based aerogels are open three-dimensional porous materials that are characterized by outstanding properties, such as a low density, high porosity and high surface area, in addition to their biocompatibility and non-cytotoxicity. Here we fabricated pure and binary blended aerogels from cellulose nanofibers (CNFs) and chitosan (CS), using a chemical-free approach that consists of high-pressure homogenization and freeze-drying. The prepared aerogels showed a different porosity and density, depending on the material and mixing ratio. The porosity and density of the aerogels ranged from 99.1 to 90.8% and from 0.0081 to 0.141 g/cm3, respectively. Pure CNFs aerogel had the highest porosity and lightest density, but it showed poor mechanical properties and a high water absorption capacity. Mixing CS with CNFs significantly enhance the mechanical properties and reduce its water uptake. The two investigated ratios of aerogel blends had superior mechanical and thermal properties over the single-material aerogels, in addition to reduced water uptake and 2-log antibacterial activity. This green fabrication and chemical-free approach could have great potential in the preparation of biopolymeric scaffolds for different biomedical applications, such as tissue-engineering scaffolds.
Thermal Conductivity of Detonation Nanodiamond Hydrogels and Hydrosols by Direct Heat Flux Measurements
Authors: Usoltseva Liliya O. , Volkov Dmitry S. , Karpushkin Evgeny A. , Korobov Mikhail V. , Proskurnin Mikhail A.
Published: December 2021

The methodology and results of thermal conductivity measurements by the heat-flow technique for the detonation nanodiamond suspension gels, sols, and powders of several brands in the range of nanoparticle concentrations of 2–100% w/w are discussed. The conditions of assessing the thermal conductivity of the fluids and gels (a FOX 50 heat-flow meter) with the reproducibility (relative standard deviation) of 1% are proposed. The maximum increase of 13% was recorded for the nanodiamond gels (140 mg mL−1 or 4% v/v) of the RDDM brand, at 0.687 ± 0.005 W m−1 K−1. The thermal conductivity of the nanodiamond powders is estimated as 0.26 ± 0.03 and 0.35 ± 0.04 W m−1 K−1 for the RUDDM and RDDM brands, respectively. The thermal conductivity for the aqueous pastes containing 26% v/v RUDDM is 0.85 ± 0.04 W m−1 K−1. The dignities, shortcomings, and limitations of this approach are discussed and compared with the determining of the thermal conductivity with photothermal-lens spectrometry.
Novel In-Frame Deletion in HTRA1 Gene, Responsible for Stroke at a Young Age and Dementia—A Case Study
Authors: Grigaitė Julija , Šiaurytė Kamilė , Audronytė Eglė , Preikšaitienė Eglė , Burnytė Birutė , Pranckevičienė Erinija , Ekkert Aleksandra , Utkus Algirdas , Jatužis Dalius
Published: December 2021

Biallelic mutations in the high-temperature requirement A serine peptidase 1 (HTRA1) gene are known to cause an extremely rare cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL), which belongs to the group of hereditary cerebral small vessel diseases and is mainly observed in the Japanese population. Even though this pathology is inherited in an autosomal recessive manner, recent studies have described symptomatic carriers with heterozygous HTRA1 mutations who have milder symptoms than patients with biallelic HTRA1 mutations. We present the case of a Lithuanian male patient who had a stroke at the age of 36, experienced several transient ischemic attacks, and developed an early onset, progressing dementia. These clinical symptoms were associated with extensive leukoencephalopathy, lacunar infarcts, and microbleeds based on brain magnetic resonance imaging (MRI). A novel heterozygous in-frame HTRA1 gene deletion (NM_002775.5:c.533_535del; NP_002766.1:p.(Lys178del)) was identified by next generation sequencing. The variant was consistent with the patient’s phenotype, which could not be explained by alternative causes, appeared highly deleterious after in silico analysis, and was not reported in the medical literature or population databases to date.
Genome-Wide Analysis of the MADS-Box Gene Family in Maize: Gene Structure, Evolution, and Relationships
Authors: Zhao Da , Chen Zheng , Xu Lei , Zhang Lijun , Zou Quan
Published: December 2021

The MADS-box gene family is one of the largest families in plants and plays an important roles in floral development. The MADS-box family includes the SRF-like domain and K-box domain. It is considered that the MADS-box gene family encodes a DNA-binding domain that is generally related to transcription factors, and plays important roles in regulating floral development. Our study identified 211 MADS-box protein sequences in the Zea mays proteome and renamed all the genes based on the gene annotations. All the 211 MADS-box protein sequences were coded by 98 expressed genes. Phylogenetic analysis of the MADS-box genes showed that all the family members were categorized into five subfamilies: MIKC-type, Mα, Mβ, Mγ, and Mδ. Gene duplications are regarded as products of several types of errors during the period of DNA replication and reconstruction; in our study all the 98 MADS-box genes contained 22 pairs of segmentally duplicated events which were distributed on 10 chromosomes. We compared expression data in different tissues from the female spikelet, silk, pericarp aleurone, ear primordium, leaf zone, vegetative meristem, internode, endosperm crown, mature pollen, embryo, root cortex, secondary root, germination kernels, primary root, root elongation zone, and root meristem. According to analysis of gene ontology pathways, we found a total of 41 pathways in which MADS-box genes in maize are involved. All the studies we conducted provided an overview of MADS-box gene family members in maize and showed multiple functions as transcription factors. The related research of MADS-box domains has provided the theoretical basis of MADS-box domains for agricultural applications.
Numerical Simulation of the Tsunami Generated by a Potential Submarine Landslide in La Palma (Canary Islands) after the September 2021 Cumbre Vieja Eruption
Authors: Periáñez Raúl
Published: December 2021

Cumbre Vieja volcano, located in La Palma Island (Canary Islands, Spain), erupted on 19 September 2021. Some papers have been published in the past in which the tsunamis generated by a potential massive landslide due to a collapse of one of the volcano flanks are investigated. However, a potential slide of the lava flow down the island slope has not been considered yet. A numerical model has been applied to simulate the propagation of the tsunami generated by such slide and to assess its consequences in the near field (Canary Islands and west coast of Africa). The model provides maps of maximum wave heights and arrival times of the tsunami, as well as time series of water surface elevation at several selected locations. Since the volume involved in such potential slide is a priori not known, several values were tested and their effects compared.