bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Smartphone Use on Sleep in Undergraduate Medical Students: A Cross-Sectional Study
Authors: Goel Ashish , Moinuddin Arsalan , Tiwari Rajesh , Sethi Yashendra , Suhail Mohammed K. , Mohan Aditi , Kaka Nirja , Sarthi Parth , Dutt Ravi , Ahmad Sheikh F. , Attia Sabry M. , Emran Talha Bin , Chopra Hitesh , Greig Nigel H.
Published: November 2023

Smartphone use, particularly at night, has been shown to provoke various circadian sleep–wake rhythm disorders such as insomnia and excessive daytime tiredness. This relationship has been mainly scrutinized among patient groups with higher rates of smartphone usage, particularly adolescents and children. However, it remains obscure how smartphone usage impacts sleep parameters in adults, especially undergraduate college students. This study sought to (1) investigate the association between smartphone use (actual screen time) and four sleep parameters: Pittsburgh sleep quality score (PSQI), self-reported screen time, bedtime, and rise time; (2) compare the seven PSQI components between good and poor sleep quality subjects. In total, 264 undergraduate medical students (aged 17 to 25 years) were recruited from the Government Doon Medical College, Dehradun, India. All participants completed a sleep questionnaire, which was electronically shared via a WhatsApp invitation link. Hierarchical and multinomial regression analyses were performed in relation to (1) and (2). The average PSQI score was 5.03 ± 0.86, with approximately one in two respondents (48.3%) having a poor sleep index. Smartphone use significantly predicted respondents’ PSQI score (β = 0.142, p = 0.040, R2 = 0.027), perceived screen time (β = 0.113, p = 0.043, R2 = 343), bedtime (β = 0.106, p = 0.042, R2 = 045), and rise time (β = 0.174, p = 0.015, R2 = 0.028). When comparing poor-quality sleep (PSQI ≥ 5) to good-quality sleep (PSQI < 5), with good-quality sleep as the reference, except sleep efficiency and sleep medications (p > 0.05), five PSQI components declined significantly: subjective sleep quality (β = −0.096, p < 0.001); sleep latency (β = −0.034, p < 0.001); sleep duration (β = −0.038, p < 0.001); sleep disturbances (β = 1.234, p < 0.001); and sleep dysfunction (β = −0.077, p < 0.001). Consequently, public health policymakers should take this evidence into account when developing guidelines around smartphone use—i.e., the when, where, and how much smartphone use—to promote improved sleep behaviour and reduce the rate of sleep–wake rhythm disorders.
Understanding Professional Medical Interpreters’ Perspectives on Advancing Accurate and Culturally Informed Patient–Provider Communication for Filipinos in Hawaiʻi: Qualitative Analysis
Authors: Kostareva Uliana , Soo Hoo Carrie A. , Zeng Suzanne M. , Albright Cheryl L. , Ceria-Ulep Clementina D. , Fontenot Holly B.
Published: November 2023

One in every eight persons in Hawaiʻi, USA, have limited English proficiency (LEP) and are entitled to free language assistance for federally funded services under Title IV of the Civil Rights Act of 1964. They also have the right to culturally and linguistically appropriate services (CLAS) provided by professional medical interpreters (PMIs). This study’s goals were to uncover barriers and facilitators of CLAS from the perspective of PMIs. PMIs for Filipino languages (n = 10) participated in an online survey and semi-structured interviews. Quantitative data were analyzed using descriptive statistics, and qualitative data were analyzed using conventional content analysis. Six themes emerged in the qualitative analysis: (1) cultural and social factors that can influence patient–provider communication; (2) barriers to effective patient–provider communication: patient, healthcare provider, and PMI levels; (3) facilitators of effective patient–provider communication: patient, healthcare provider, and PMI levels; (4) COVID-19 and remote interpreting barriers and facilitators; (5) strengths and weaknesses of in-person and stand-by interpreting appointments; and, (6) recommendations: system and provider levels. Proposed interventions could include advertising language services among Filipino communities and educating them about their language rights, providing additional resources for language assistance, employing more PMIs, training staff/providers, and supporting the use of PMIs versus untrained individuals.
Lactoferrin, the Moonlighting Protein of Innate Immunity
Authors: Cutone Antimo , Musci Giovanni , Bonaccorsi di Patti Maria Carmela
Published: November 2023

Lactoferrin (Lf), a naturally occurring glycoprotein involved in innate immunity, was first discovered in bovine milk [...]
Effects and Mechanisms of Non-Thermal Plasma-Mediated ROS and Its Applications in Animal Husbandry and Biomedicine
Authors: Yang Yuhan , Wang Yuan , Wei Shang , Wang Xianzhong , Zhang Jiaojiao
Published: November 2023

Non-thermal plasma (NTP) is an ionized gas composed of neutral and charged reactive species, electric fields, and ultraviolet radiation. NTP presents a relatively low discharge temperature because it is characterized by the fact that the temperature values of ions and neutral particles are much lower than that of electrons. Reactive species (atoms, radicals, ions, electrons) are produced in NTP and delivered to biological objects induce a set of biochemical processes in cells or tissues. NTP can mediate reactive oxygen species (ROS) levels in an intensity- and time-dependent manner. ROS homeostasis plays an important role in animal health. Relatively low or physiological levels of ROS mediated by NTP promote cell proliferation and differentiation, while high or excessive levels of ROS mediated by NTP cause oxidative stress damage and even cell death. NTP treatment under appropriate conditions not only produces moderate levels of exogenous ROS directly and stimulates intracellular ROS generation, but also can regulate intracellular ROS levels indirectly, which affect the redox state in different cells and tissues of animals. However, the treatment condition of NTP need to be optimized and the potential mechanism of NTP-mediated ROS in different biological targets is still unclear. Over the past ten decades, interest in the application of NTP technology in biology and medical sciences has been rapidly growing. There is significant optimism that NTP can be developed for a wide range of applications such as wound healing, oral treatment, cancer therapy, and biomedical materials because of its safety, non-toxicity, and high efficiency. Moreover, the combined application of NTP with other methods is currently a hot research topic because of more effective effects on sterilization and anti-cancer abilities. Interestingly, NTP technology has presented great application potential in the animal husbandry field in recent years. However, the wide applications of NTP are related to different and complicated mechanisms, and whether NTP-mediated ROS play a critical role in its application need to be clarified. Therefore, this review mainly summarizes the effects of ROS on animal health, the mechanisms of NTP-mediated ROS levels through antioxidant clearance and ROS generation, and the potential applications of NTP-mediated ROS in animal growth and breeding, animal health, animal-derived food safety, and biomedical fields including would healing, oral treatment, cancer therapy, and biomaterials. This will provide a theoretical basis for promoting the healthy development of animal husbandry and the prevention and treatment of diseases in both animals and human beings.
Deficiency of ChPks and ChThr1 Inhibited DHN-Melanin Biosynthesis, Disrupted Cell Wall Integrity and Attenuated Pathogenicity in Colletotrichum higginsianum
Authors: Duan Lingtao , Wang Li , Chen Weilun , He Zhenrui , Zhou Erxun , Zhu Yiming
Published: November 2023

Colletotrichum higginsianum is a major pathogen causing anthracnose in Chinese flowering cabbage (Brassica parachinensis), posing a significant threat to the Chinese flowering cabbage industry. The conidia of C. higginsianum germinate and form melanized infection structures called appressoria, which enable penetration of the host plant’s epidermal cells. However, the molecular mechanism underlying melanin biosynthesis in C. higginsianum remains poorly understood. In this study, we identified two enzymes related to DHN-melanin biosynthesis in C. higginsianum: ChPks and ChThr1. Our results demonstrate that the expression levels of genes ChPKS and ChTHR1 were significantly up-regulated during hyphal and appressorial melanization processes. Furthermore, knockout of the gene ChPKS resulted in a blocked DHN-melanin biosynthetic pathway in hyphae and appressoria, leading to increased sensitivity of the ChpksΔ mutant to cell-wall-interfering agents as well as decreased turgor pressure and pathogenicity. It should be noted that although the Chthr1Δ mutant still exhibited melanin accumulation in colonies and appressoria, its sensitivity to cell-wall-interfering agents and turgor pressure decreased compared to wild-type strains; however, complete loss of pathogenicity was not observed. In conclusion, our results indicate that DHN-melanin plays an essential role in both pathogenicity and cell wall integrity in C. higginsianum. Specifically, ChPks is crucial for DHN-melanin biosynthesis while deficiency of ChThr1 does not completely blocked melanin production.
Bacillus amyloliquefaciens AK-12 Helps Rapeseed Establish a Protection against Brevicoryne brassicae
Authors: Qian Shixiong , Ahmed Ayesha , He Pengbo , He Pengfei , Munir Shahzad , Xia Mengyuan , Tang Chaoyun , Tang Ping , Wang Zaiqiang , Khan Rizwan , Li Xingyu , Wu Yixin , He Yueqiu
Published: November 2023

Aphids are a serious threat to rapeseed (Brassica napus L.) production, and cause unmanageable loss. Therefore, effective prevention and management strategies are urgently required to avoid losses. Bacillus amyloliquefaciens AK-12 isolated from a dead aphid with aphicidal activity was tagged with a green fluorescent protein through a natural transformation. The transformed strains were checked for stability and growth, and the best-performing strain was tested for its colonization inside and outside the rapeseed plant. The stability of AK-12-GFP reached more than 95%, and the growth curve was consistent with that of AK-12. After 30 days of treatment, the colonization of 1 × 106 CFU/g was recorded in rapeseed leaves. Interestingly, AK-12 reduced the aphid transmission rate compared with the control and improved the growth of the rapeseed seedlings. Meanwhile, the AK-12 strain also exhibited phosphorus, potassium-solubilizing, and nitrogen-fixing activity, and produced 2.61 µg/mL of IAA at 24 h. Regulation in the activity of four enzymes was detected after the AK-12 treatment. Phenylalanine ammonia lyase (PAL) was recorded at a maximum of 86.84 U/g after 36 h, and catalase (CAT) decreased after 48 h; however, peroxidase (POD) and polyphenol oxidase (PPO) reached the maximum within 12 h of AK-12 application. Additionally, important resistance genes related to these enzymes were upregulated, indicating the activation of a defense response in the rapeseed against aphids. In conclusion, defense enzymes and defense-related gene activation could improve the pest resistance in rapeseed, which has good application prospects for the future to be developed into biopesticide.
Efficiency of Platelet Transfusion in Patients with Moderate-to-Severe Chronic Kidney Disease and Thrombocytopenia
Authors: Ali Sevigean , Botnarciuc Mihaela , Daba Lavinia Carmen , Ispas Sorina , Stanigut Alina Mihaela , Pana Camelia , Burcila Marian-Catalin , Tuta Liliana-Ana
Published: November 2023

There have been relatively few studies revealing a decreased platelet count in chronic kidney disease (CKD). Although this hematological abnormality is not as well documented as renal anemia, platelet functions are altered in the uremic environment and there is an increased risk of bleeding. The aim of this study was to assess the effectiveness of the administration of platelet concentrate in CKD based on how patient prognosis was influenced by platelet transfusion therapy. The study monitored 104 patients with CKD and thrombocytopenia who received platelet transfusion during their hospitalization in the period from 2015 to 2021. The complete blood cell count, serum urea and creatinine, and inflammatory status were tested upon admission. The number of transfused platelet units were considered for each patient. A Kruskal–Wallis H test showed that for one transfused platelet unit, the distribution of the number of platelets (×103/µL) was the same across the categories of associated diagnoses, which was seen as possible risk factors for thrombocytopenia, including liver cirrhosis and urosepsis. With a single exception, all patients exceeded the critical threshold of 20 × 103/µL and 14 patients remained under 50 × 103/µL. Even though our patients exceeded the critical threshold of platelet numbers, in patients with multiple comorbidities, severe, uncontrolled hemorrhages could not be prevented in 4.83% of cases.
Breast Cancer Chemoresistance: Insights into the Regulatory Role of lncRNA
Authors: Ahmadpour Seyedeh Tayebeh , Orre Charlotte , Bertevello Priscila Silvana , Mirebeau-Prunier Delphine , Dumas Jean-François , Desquiret-Dumas Valérie
Published: November 2023

Long noncoding RNAs (lncRNAs) are a subclass of noncoding RNAs composed of more than 200 nucleotides without the ability to encode functional proteins. Given their involvement in critical cellular processes such as gene expression regulation, transcription, and translation, lncRNAs play a significant role in organism homeostasis. Breast cancer (BC) is the second most common cancer worldwide and evidence has shown a relationship between aberrant lncRNA expression and BC development. One of the main obstacles in BC control is multidrug chemoresistance, which is associated with the deregulation of multiple mechanisms such as efflux transporter activity, mitochondrial metabolism reprogramming, and epigenetic regulation as well as apoptosis and autophagy. Studies have shown the involvement of a large number of lncRNAs in the regulation of such pathways. However, the underlying mechanism is not clearly elucidated. In this review, we present the principal mechanisms associated with BC chemoresistance that can be directly or indirectly regulated by lncRNA, highlighting the importance of lncRNA in controlling BC chemoresistance. Understanding these mechanisms in deep detail may interest the clinical outcome of BC patients and could be used as therapeutic targets to overcome BC therapy resistance.
Antioxidant Iron Oxide Nanoparticles: Their Biocompatibility and Bioactive Properties
Authors: Lee Jaewook , Lee Ji-Heon , Lee Seung-Yeul , Park Sin A , Kim Jae Hoon , Hwang Dajeong , Kim Kyung A , Kim Han Sang
Published: November 2023

A lot of nanomaterials have been applied to various nano-biotechnological fields, such as contrast agents, drug or gene delivery systems, cosmetics, and so on. Despite the expanding usage of nanomaterials, concerns persist regarding their potential toxicity. To address this issue, many scientists have tried to develop biocompatible nanomaterials containing phytochemicals as a promising solution. In this study, we synthesized biocompatible nanomaterials by using gallic acid (GA), which is a phytochemical, and coating it onto the surface of iron oxide nanoparticles (IONPs). Importantly, the GA-modified iron oxide nanoparticles (GA-IONPs) were successfully prepared through environmentally friendly methods, avoiding the use of harmful reagents and extreme conditions. The presence of GA on the surface of IONPs improved their stability and bioactive properties. In addition, cell viability assays proved that GA-IONPs possessed excellent biocompatibility in human dermal papilla cells (HDPCs). Additionally, GA-IONPs showed antioxidant activity, which reduced intracellular reactive oxygen species (ROS) levels in an oxidative stress model induced by hydrogen peroxide (H2O2). To investigate the impact of GA-IONPs on exosome secretions from oxidative stress-induced cells, we analyzed the number and characteristics of exosomes in the culture media of HDPCs after H2O2 stimulation or GA-IONP treatment. Our analysis revealed that both the number and proportions of tetraspanins (CD9, CD81, and CD63) in exosomes were similar in the control group and the GA-IONP-treated groups. In contrast, exosome secretion was increased, and the proportion of tetraspanin was changed in the H2O2-treated group compared to the control group. It demonstrated that treatment with GA-IONPs effectively attenuated exosome secretion induced by H2O2-induced oxidative stress. Therefore, this GA-IONP exhibited outstanding promise for applications in the field of nanobiotechnology.
Plant Responses to Climate Change
Authors: Marques Isabel , Ramalho José C. , Ribeiro-Barros Ana I.
Published: November 2023

Ongoing climate change poses a great risk to the natural environment and the sustainability of agriculture [...]
The Tolerogenic Influence of Dexamethasone on Dendritic Cells Is Accompanied by the Induction of Efferocytosis, Promoted by MERTK
Authors: Li Vivien , Binder Michele D. , Kilpatrick Trevor J.
Published: November 2023

Many treatments for autoimmune diseases, caused by the loss of immune self-tolerance, are broadly immunosuppressive. Dendritic cells (DCs) can be induced to develop anti-inflammatory/tolerogenic properties to suppress aberrant self-directed immunity by promoting immune tolerance in an antigen-specific manner. Dexamethasone can generate tolerogenic DCs and upregulates MERTK expression. As MERTK can inhibit inflammation, we investigated whether dexamethasone’s tolerogenic effects are mediated via MERTK, potentially providing a novel therapeutic approach. Monocyte-derived DCs were treated with dexamethasone, and with and without MERTK ligands or MERTK inhibitors. Flow cytometry was used to assess effects of MERTK modulation on co-stimulatory molecule expression, efferocytosis, cytokine secretion and T cell proliferation. The influence on expression of Rab17, which coordinates the diversion of efferocytosed material away from cell surface presentation, was assessed. Dexamethasone-treated DCs had upregulated MERTK expression, decreased expression of co-stimulatory molecules, maturation and proliferation of co-cultured T cells and increased uptake of myelin debris. MERTK ligands did not potentiate these properties, whilst specific MERTK inhibition only reversed dexamethasone’s effect on myelin uptake. Cells undergoing efferocytosis had higher Rab17 expression. Dexamethasone-enhanced efferocytosis in DCs is MERTK-dependent and could exert its tolerogenic effects by increasing Rab17 expression to prevent the presentation of efferocytosed material on the cell surface to activate adaptive immune responses.
Polyphenol Extraction from Food (by) Products by Pulsed Electric Field: A Review
Authors: Athanasiadis Vassilis , Chatzimitakos Theodoros , Kotsou Konstantina , Kalompatsios Dimitrios , Bozinou Eleni , Lalas Stavros I.
Published: November 2023

Nowadays, more and more researchers engage in studies regarding the extraction of bioactive compounds from natural sources. To this end, plenty of studies have been published on this topic, with the interest in the field growing exponentially. One major aim of such studies is to maximize the extraction yield and, simultaneously, to use procedures that adhere to the principles of green chemistry, as much as possible. It was not until recently that pulsed electric field (PEF) technology has been put to good use to achieve this goal. This new technique exhibits many advantages, compared to other techniques, and they have successfully been reaped for the production of extracts with enhanced concentrations in bioactive compounds. In this advancing field of research, a good understanding of the existing literature is mandatory to develop more advanced concepts in the future. The aim of this review is to provide a thorough discussion of the most important applications of PEF for the enhancement of polyphenols extraction from fresh food products and by-products, as well as to discuss the current limitations and the prospects of the field.
A Protein–Protein Interaction Analysis Suggests a Wide Range of New Functions for the p21-Activated Kinase (PAK) Ste20
Authors: Joshua Ifeoluwapo Matthew , Lin Meng , Mardjuki Ariestia , Mazzola Alessandra , Höfken Thomas
Published: November 2023

The p21-activated kinases (PAKs) are important signaling proteins. They contribute to a surprisingly wide range of cellular processes and play critical roles in a number of human diseases including cancer, neurological disorders and cardiac diseases. To get a better understanding of PAK functions, mechanisms and integration of various cellular activities, we screened for proteins that bind to the budding yeast PAK Ste20 as an example, using the split-ubiquitin technique. We identified 56 proteins, most of them not described previously as Ste20 interactors. The proteins fall into a small number of functional categories such as vesicle transport and translation. We analyzed the roles of Ste20 in glucose metabolism and gene expression further. Ste20 has a well-established role in the adaptation to changing environmental conditions through the stimulation of mitogen-activated protein kinase (MAPK) pathways which eventually leads to transcription factor activation. This includes filamentous growth, an adaptation to nutrient depletion. Here we show that Ste20 also induces filamentous growth through interaction with nuclear proteins such as Sac3, Ctk1 and Hmt1, key regulators of gene expression. Combining our observations and the data published by others, we suggest that Ste20 has several new and unexpected functions.
Role of Surface Defects on Photoinduced Reactivity in SiO2 Nanoparticles
Authors: D’Amato Roberto , Quaglia Giulia , Selvaggi Roberta , Marmottini Fabio , Latterini Loredana
Published: November 2023

Silica nanostructured materials find application in different fields, since they are cheap, versatile, and easy to functionalize as materials. However, silica reactivity has not been deeply investigated yet, mainly due to a poor understanding of how it is affected by superficial defects. In the present study, the electronic and optical properties of nanoparticles have been investigated using bare silica nanoparticles (SNP) and amino- or phosphonate-functionalized silica nanoparticles (SNP–APTES and SNP-phosphonate), prepared by a sol-gel procedure, and their morphology has been investigated using transmission electron microscopy (TEM) analysis. The prepared silica nanomaterials were characterized by means of reflectance and emission spectroscopies to determine the types of defects that can be found on silica nanoparticles’ surface. In order to understand the effect of surface defects on the reactivity of silica, the nanoparticles were employed for the photocatalytic degradation of Rhodamine 6G (R6G), upon selective irradiation at 320 nm, where only silica colloids absorb. The photoreaction was carried out in ethanol and in water and was monitored following the fluorescence signal of the dye. The evaluation of the fluorescent intensities allowed for the determination of the degradation efficiencies.
Muscle Strength and Joint Range of Motion of the Spine and Lower Extremities in Female Prepubertal Elite Rhythmic and Artistic Gymnasts
Authors: Mandroukas Athanasios , Metaxas Ioannis , Michailidis Yiannis , Metaxas Thomas
Published: November 2023

The purpose of this study was to investigate and compare the passive joint range of motion (PROM) and muscle strength in prepubertal rhythmic gymnasts (RGs), artistic gymnasts (AGs), and a control group (CG) of the same age. A total of 54 prepubertal girls were divided into three groups: 18 RGs (age 11.14 ± 0.7, height 142.6 ± 5.81, and body mass 31.2 ± 3.63); 18 AGs (age 11.27 ± 0.99, height 139.6 ± 5.85, and body mass 31.7 ± 3.21), and 18 school girls who are defined as CG (age 10.55 ± 0.42, height 145.33 ± 6.95, and body mass 42.1 ± 8.21) participated in the study. All athletes were elites and participated in national competitions. The CG participated only in their school physical education program. Isokinetic peak torques were measured using an isokinetic dynamometer (Cybex II) at 60, 180, and 300°·sec−1. Body mass index was greater in the CG compared to RGs and AGs (p < 0.001). PROM in cervical extension in RG was significantly higher compared to the AG and CG (p < 0.001). The athlete groups, RG and AG, showed significantly greater PROM in knee flexion (p < 0.001), hip flexion (p < 0.001), and hip abduction (p < 0.05) compared to CG. PROM in hip flexion was different between the left and right leg in RGs. The relative muscle strength of the quadriceps in the RG and AG was significantly greater compared to CG (p < 0.001 and p < 0.01 respectively). Gymnastics training in prepubertal ages can improve neuromuscular function and increase the relative muscle strength. Therefore, it is essential to note that when evaluating children within the developmental ages, especially those involved in sports, the type of muscle strength to be assessed should be specified.
Synergistic Effects of Multiple Environmental Factors on Degradation of Silicone Rubber Seals under Marine Atmosphere
Authors: Wang Rui-Yuan , Wang Chong-Hao , Wang Ying , Zhang Wei-Fang
Published: November 2023

In this research, the degradation behavior and failure mechanism of silicone rubber seal rings under the synergistic effects of multiple factors in the marine atmosphere are fully investigated. Firstly, four aging factors of air, temperature, compressive stress, and chemical medium were determined by analyzing the service environment profile of silicone rubber seal under a marine atmosphere environment. Secondly, to better simulate the actual service environment of silicone rubber and shorten the test period, an artificially accelerated aging test was designed and carried out in the laboratory. In this paper, temperature is utilized as the accelerating stress. According to the results of the pre-test, the accelerating stress level is finally determined to be 110–150 ∘C. In addition, the compression set applied is consistent with the constant compression permanent deformation value of 28% of the silicone rubber in the actual service process. Finally, through the macroscopic physical properties and microstructure analysis of the samples before and after aging, the corresponding test results are given, and the failure mechanism is analyzed and discussed in detail. Through the above test results and discussion, it can be concluded that the aging process of multi-factor coupling on the lower silicone rubber seal ring is uneven, and its aging process is not a simple superposition of multiple environmental factors. More importantly, the above test data and results are of great significance for evaluating the service life of silicone rubber seals, which can be utilized in the future to improve the reliability and durability of related equipment in the marine environment.
Effect of Polyoxymethylene Fiber on the Mechanical Properties and Abrasion Resistance of Ultra-High-Performance Concrete
Authors: Tan Lixin , Yang Jun , Li Chuanxi , Zhang Gaozhan , Ding Qingjun , Sun Daosheng , Zhang Yongyuan
Published: November 2023

It is necessary to prepare marine UHPC with synthetic fibers instead of steel fibers, owing to the corrosion risk of steel fibers in marine environments. Currently, the performance of UHPC prepared with different types of fibers has not been comparatively investigated. This work prepared UHPC with steel fiber, polyoxymethylene (POM) fiber, polypropylene (PP) fiber, and polyvinyl alcohol (PVA) fiber. The effects of different fibers on the mechanical properties, impact, and abrasion resistance of UHPC were studied and compared. The results showed that increasing POM fiber can increase the mechanical strength, flexural toughness, impact, and abrasion resistance of UHPC. When its content reaches 2%, the adsorbed-in-fracture energy and abrasion strength of UHPC are 2670 J and 105 h/(kg/m2), respectively. At the same fiber content, POM fiber-reinforced UHPC shows better mechanical strength, toughness, and impact- and abrasion-resistance than the polypropylene (PP)- and polyvinyl alcohol (PVA)-fiber-reinforced UHPCs. Microstructure investigation found that PP fiber has the weakest binding with UHPC paste, which would directly pull out of the matrix under external tensile loading. This weak connection limits the strengthening and toughening effect on the UHPC. PVA fiber has an excellent interfacial connection with the UHPC paste. However, the low tensile strength of PVA fiber limits the strength and toughness of UHPC. POM fiber has a high tensile strength and can absorb tensile loading through debonding, fracture, and tearing. The fracture interface of POM fiber is large, indicating its significant role in strengthening and toughening the UHPC.
Enhanced Photosynthetic Pigment Production Using a Scaled-Up Continuously Circulated Bioreactor
Authors: Lee Won-Kyu , Ryu Yong-Kyun , Kim Taeho , Park Areumi , Lee Yeon-Ji , Sunwoo In Yung , Koh Eun-Jeong , Oh Chulhong , Choi Woon-Yong , Kang Do-Hyung
Published: November 2023

Microalgae have gained attention as a promising source of chlorophylls and carotenoids in various industries. However, scaling up of conventional bubble columns presents challenges related to cell sedimentation and the presence of non-photosynthetic cells due to non-circulating zones and decreased light accessibility, respectively. Therefore, this study aimed to evaluate the newly developed continuously circulated bioreactor ROSEMAX at both laboratory and pilot scales, compared to a conventional bubble column. There was no significant difference in the biomass production and photosynthetic pigment content of Tetraselmis sp. cultivated at the laboratory scale (p > 0.05). However, at the pilot scale, the biomass cultured in ROSEMAX showed significantly high biomass (1.69 ± 0.11 g/L, dry weight, DW), chlorophyll-a (14.60 ± 0.76 mg/g, DW), and total carotene (5.64 ± 0.81 mg/g, DW) concentrations compared to the conventional bubble column (1.17 ± 0.11 g/L, DW, 10.67 ± 0.72 mg/g, DW, 3.21 ± 0.56 mg/g, DW, respectively) (p ≤ 0.05). Flow cytometric analyses confirmed that the proportion of Tetraselmis sp. live cells in the culture medium of ROSEMAX was 32.90% higher than that in the conventional bubble column, with a photosynthetic efficiency 1.14 times higher. These results support suggestions to use ROSEMAX as a bioreactor for industrial-scale applications.
Frequency of Impulsive-Compulsive Behavior and Associated Psychological Factors in Parkinson’s Disease: Lack of Control or Too Much of It?
Authors: Kapsomenakis Alexandros , Kasselimis Dimitrios , Vaniotis Emily , Bougea Anastasia , Koros Christos , Simitsi Athina Maria , Stefanis Leonidas , Potagas Constantin
Published: November 2023

Background and Objectives: Impulse Control Disorders (ICDs) including pathological gambling, hypersexuality, compulsive eating, compulsive buying, and other related behaviors are well-known distinct non-motor symptoms in Parkinson’s Disease (PD). Some large-scale studies present a prevalence of at least 10%, however, there are other reports providing much higher rates. The majority of the conducted studies investigating ICDs focus mainly on pharmacological factors, however, from a psychological perspective, there is yet enough room for investigation. In order to address the above issues, we designed a two-part study. Materials and Methods: First, we aimed to identify the incidence of ICD and related behaviors in a cohort of 892 Greek PD patients. Second, we administered a comprehensive battery of psychometric tools to assess psychological factors such as personality dimensions, quality of life, defenses, coherence, and resilience as well as to screen general cognitive capacity in PD patients with ICD manifestations. Results: With regard to the first part, we identified ICD manifestations in 12.4% of the patients. Preliminary findings from the second part indicate elevated activity, rather than impulsivity, as well as interrelations between several variables, including measures of activity, coping mechanisms, and quality of life. Conclusions: We present a working hypothesis for the contribution of high activity channeled to specific behavioral patterns through specific coping mechanisms, concerning the emergence of ICDs and related behaviors in PD, and further stress the importance of compulsivity rather than impulsivity in this process.
Endpoint Detection Based on Optical Method in Chemical Mechanical Polishing
Authors: Tian Fangxin , Wang Tongqing , Lu Xinchun
Published: November 2023

Endpoint detection is an important technology in chemical mechanical polishing (CMP), which is used to capture the material interface and compensate the variations of consumables and incoming wafer thickness. This paper aimed to apply optical detection in metal CMP. An in situ optical measurement system was developed for a 12-inch CMP tool. Kinematic analysis of the scanning trajectory of the laser device indicated the relative position relationship between the device and the wafer. Average optical data within the wafer described the material removal of metal CMP. Furthermore, optical data and location described the non-uniformity of the entire wafer surface. In this research, the polishing condition and the residual of the wafer edge are characterized by optical trace. Pauta Criterion is used to discriminate the inflexion point of the material interface. The results reveal that the interface capture is accurate and effective.
Method for Extraction and Evaluation of Heliocarpus popayanensis and Triumfetta bogotensis as Natural Coagulants for the Treatment of Wastewater
Authors: Garcés-Gómez Yeison Alberto , Pacheco-Gonzalez Sebastián Isaac
Published: November 2023

This research evaluates extracts from the bark of Heliocarpus popayanensis and Triumfetta bogotensis as coagulating agents for removing turbidity in domestic wastewater, considering the coagulant dosage and pH of the wastewater. ANOVA was conducted to assess differences between the coagulants, dosages, and pH, with three pH levels (5, 8, and 9) and six dosages (7, 9, 11, 13, 15, and 17 mL per 1000 mL of wastewater) at a significance level of α = 0.05, and both the p-value and effect size were evaluated. This study found that the mucilaginous compound from the bark of Triumfetta bogotensis performed better in reducing turbidity levels, with an average reduction of 30.2 NTU (Nephelometric Turbidity Unit) (CI [25.9 NTU; 34.5 NTU], α = 0.05) at a pH of 5, and an average initial NTU of 102.2. This represents an average reduction of 70.45%. The dosage factor did not show significant effects on turbidity reduction, which opens the possibility for further study to determine the optimal dosage of the best coagulant.
A Case Series of Four Patients with Artery of Percheron Occlusion over a Three-Month Period
Authors: Perovnik Matej , Pretnar Oblak Janja , Frol Senta
Published: November 2023

Here, we present a case series of four patients diagnosed with acute ischaemic stroke due to occlusion of the artery of Percheron (AOP), a rare stroke variant, observed in a single emergency centre within a three-month period. AOP occlusion is characterized by bilateral thalamic infarction with or without involvement of the mesencephalon. The presenting symptoms are diverse and not specific, but commonly include disturbance of consciousness, memory impairment, and vertical gaze palsy. In addition, due to the location of the infarction, imaging recognition is challenging and AOP occlusion often remains undiagnosed. This paper emphasizes the necessity of early recognition and appropriate management of AOP occlusion to significantly impact patient outcomes. Moreover, we argue that the condition might be more common than previously thought and that misdiagnosis or delay in diagnosis may lead to inappropriate treatment and potential failure to apply thrombolysis within the required timeframe.
Nutritional Intervention in Cushing’s Disease: The Ketogenic Diet’s Effects on Metabolic Comorbidities and Adrenal Steroids
Authors: Guarnotta Valentina , Amodei Roberta , Di Gaudio Francesca , Giordano Carla
Published: November 2023

Background: a very low-calorie ketogenic diet (VLCKD) is associated with improvement of metabolic and cardiovascular disorders. We aimed to evaluate the effects of a VLCKD in patients with Cushing’s disease (CD) as adjunctive therapy to treatment for the primary disease. Methods: we evaluated clinical, hormonal and metabolic parameters in 15 patients with CD and 15 controls at baseline after 1 week and 3 weeks of VLCKD and, further, after 2 weeks of a low-carbohydrate ketogenic diet (LCKD). Results: after 5 weeks of diet, a significant decrease in BMI (p = 0.002), waist circumference (WC) (p = 0.024), systolic blood pressure (p = 0.015), diastolic blood pressure (p = 0.005), ACTH (p = 0.026), cortisone (p = 0.025), total cholesterol (p = 0.006), LDL cholesterol (p = 0.017), triglycerides (p = 0.016) and alkaline phosphatase (p = 0.008) and a significant increase in HDL cholesterol (p = 0.017), vitamin D (p = 0.015) and oral disposition index (oDI) (p = 0.004) was observed in the CD patients. A significant decrease in BMI (p = 0.003), WC (p = 0.002), systolic blood pressure (p = 0.025), diastolic (p = 0.007) blood pressure and total cholesterol (p = 0.026) and an increase in HDL cholesterol (p = 0.001) and oDI (p < 0.001) was observed in controls. Conclusions: the current study confirms that a ketogenic diet is effective in improving metabolic disorders in CD and shows that a nutritional approach may be combined with conventional CD therapy in order to improve metabolic and cardiovascular comorbidities.
Deficiency of apoA-IV in Female 129X1/SvJ Mice Leads to Diet-Induced Obesity, Insulin Resistance, and Decreased Energy Expenditure
Authors: Qu Jie , Wu Dong , Ko Chih-Wei , Zhu Qi , Liu Min , Tso Patrick
Published: November 2023

Obesity is one of the main risk factors for cardiovascular diseases, type II diabetes, hypertension, and certain cancers. Obesity in women at the reproductive stage adversely affects contraception, fertility, maternal well-being, and the health of their offspring. Being a major protein component in chylomicrons and high-density lipoproteins, apolipoprotein A-IV (apoA-IV) is involved in lipid metabolism, food intake, glucose homeostasis, prevention against atherosclerosis, and platelet aggregation. The goal of the present study is to determine the impact of apoA-IV deficiency on metabolic functions in 129X1/SvJ female mouse strain. After chronic high-fat diet feeding, apoA-IV−/− mice gained more weight with a higher fat percentage than wild-type (WT) mice, as determined by measuring their body composition. Increased adiposity and adipose cell size were also observed with a microscope, particularly in periovarian fat pads. Based on plasma lipid and adipokine assays, we found that obesity in apoA-IV−/− mice was not associated with hyperlipidemia but with higher leptin levels. Compared to WT mice, apoA-IV deficiency displayed glucose intolerance and elevated insulin levels, according to the data of the glucose tolerance test, and increased HOMA-IR values at fasting, suggesting possible insulin resistance. Lastly, we found obesity in apoA-IV−/− mice resulting from reduced energy expenditure but not food intake. Together, we established a novel and excellent female mouse model for future mechanistic study of obesity and its associated comorbidities.
Order, Chaos and Born’s Distribution of Bohmian Particles
Authors: Tzemos Athanasios C. , Contopoulos George
Published: November 2023

We study order, chaos and ergodicity in the Bohmian trajectories of a 2D quantum harmonic oscillator. We first present all the possible types (chaotic, ordered) of Bohmian trajectories in wavefunctions made of superpositions of two and three energy eigenstates of the oscillator. There is no chaos in the case of two terms and in some cases of three terms. Then, we show the different geometries of nodal points in bipartite Bohmian systems of entangled qubits. Finally, we study multinodal wavefunctions and find that a large number of nodal points does not always imply the dominance of chaos. We show that, in some cases, the Born distribution is dominated by ordered trajectories, something that has a significant impact on the accessibility of Born’s rule P=|Ψ|2 by initial distributions of Bohmian particles with P0≠|Ψ0|2.
The Essential Oil from Conyza bonariensis (L.) Cronquist (Asteraceae) Exerts an In Vitro Antimelanoma Effect by Inducing Apoptosis and Modulating the MAPKs, NF-κB, and PKB/AKT Signaling Pathways
Authors: Ferreira Rafael Carlos , Duarte Sâmia Sousa , de Sousa Valgrícia Matias , de Souza Ramon Ramos Marques , Marques Karinne Kelly Gadelha , de Abrantes Renata Albuquerque , do Nascimento Yuri Mangueira , de Sousa Natália Ferreira , Scotti Marcus Tullius , Scotti Luciana , Tavares Josean Fechine , Gonçalves Juan Carlos Ramos , da Silva Marcelo Sobral , Sobral Marianna Vieira
Published: November 2023

The characterization and cytotoxicity of the essential oil from Conyza bonariensis (L.) aerial parts (CBEO) were previously conducted. The major compound was (Z)-2-lachnophyllum ester (EZ), and CBEO exhibited significant ROS-dependent cytotoxicity in the melanoma cell line SK-MEL-28. Herein, we employed the Molegro Virtual Docker v.6.0.1 software to investigate the interactions between the EZ and Mitogen-Activated Protein Kinases (MAPKs), the Nuclear Factor kappa B (NF-κB), and the Protein Kinase B (PKB/AKT). Additionally, in vitro assays were performed in SK-MEL-28 cells to assess the effect of CBEO on the cell cycle, apoptosis, and these signaling pathways by flow cytometry and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using MAPKs inhibitors. CBEO induced a significant increase in the sub-G1 peak, as well as biochemical and morphological changes characteristic of apoptosis. The in-silico results indicated that EZ interacts with Extracellular Signal-Regulated Kinase 1 (ERK1), c-Jun N-terminal Kinase 1 (JNK1), p38α MAPK, NF-κB, and PKB/AKT. Moreover, CBEO modulated the ERK1/2, JNK, p38 MAPK, NF-κB, and PKB/AKT activities in SK-MEL-28 cells. Furthermore, CBEO’s cytotoxicity against SK-MEL-28 cells was significantly altered in the presence of MAPKs inhibitors. These findings support the in vitro antimelanoma effect of CBEO through apoptosis induction, and the modulation of ERK, JNK, p38 MAPK, NF-κB, and PKB/AKT activities.
The Practical Price of Pyrrhonism
Authors: Byrd Jeremy
Published: November 2023

Sextus Empiricus presents Pyrrhonism as a skeptical lifestyle that is appealing, in large part, because of the tranquility it appears to afford. Addressing concerns about the practicality of such a lifestyle, Sextus suggests that Pyrrhonists can lead sufficiently ordinary lives while suspending belief about everything unclear. Here, I aim to offer a partial examination of the practicality and appeal of Pyrrhonism from the Pyrrhonist’s perspective. In particular, I examine how a skeptic would likely respond if asked to consider his potential use of problematic concepts in his daily life. I argue that, even if the Pyrrhonist’s skepticism is limited to certain types of controversial theoretical commitments, consideration of this issue would likely still cause him to worry that he is relying on beliefs about things unclear in his ordinary life. Along the way, I also hope to highlight some of the difficulties that a philosophically reflective person is likely to encounter if he is resistant to taking on philosophical commitments.
Advancing Paper Industry Applications with Extruded Cationic Wheat Starch as an Environmentally Friendly Biopolymer
Authors: Tara Ahmed
Published: November 2023

Within the domain of starch modification, the study delved into cationization of wheat starch through a laboratory-scale twin-screw extruder, exploring various processing conditions. Cationic starch, a crucial component for enhancing paper attributes like dry strength and printability, took center stage. The focus shifted towards integration into papermaking, investigating the transformative potential of reactive extrusion. By contrasting it with conventional dry-process methodology, innovative strides were unveiled. The study extended to pilot-scale extrusion, bridging the gap between laboratory experimentation and potential industrial implementation. Infused with scientific rigor, the investigation navigated the benefits brought about by reactive extrusion. Empirical insights highlighted a significant reduction in the intrinsic viscosity of extruded starch, decreasing from 170 mL·g−1 (native starch) to 100 mL·g−1 at a specific mechanical energy (SME) input of 800 kWh·t−1, demonstrating remarkable stability despite increased mechanical treatment. Moreover, beyond the critical threshold of 220 kWh·t−1, retention efficiency reached a stable plateau at 78%. The study revealed that utilizing a larger extruder slightly improved the mechanical properties of the paper, emphasizing the advantage of scaling up the production process and the consistency of results across different extruder sizes.
Decision Making in a Closed-Loop Supply Chain with a Waste Management Program: Manufacturers’ Take-Back Activity and Governmental Subsidies for Remanufacturing
Authors: Lee Doo-Ho , Park Eun-Hee
Published: November 2023

As awareness of climate change increases, diverse initiatives such as subsidies for remanufactured products and take-back programs for end-of-life products have been taken to conserve energies and materials. This paper explores how the subsidy program affects manufacturer’s take-back activity in a closed-loop supply chain and also analyzes how a coalition between a retailer and a remanufacturer affects the equilibrium decisions. Major findings of this paper reveal that (i) when a take-back program is implemented, the government imposes a high penalty on products that are not collected, thereby encouraging manufacturers to collect more used products, (ii) implementing a take-back program in conjunction with a subsidy program results in a greater reduction in environmentally negative impacts and an enhanced social welfare compared to implementing them separately, and (iii) a coalition between a retailer and a remanufacturer results in lowering the penalty imposed to a manufacturer, which leads to lowering the quantity of the collected and remanufactured products.
Satellite Monitoring of the Urban Expansion in the Pearl River–Xijiang Economic Belt and the Progress towards SDG11.3.1
Authors: Liu Shuyue , Yan Yan , Hu Baoqing
Published: November 2023

Quantitative analysis of the spatiotemporal pattern of urban expansion and forecasting of the progress towards SDG11.3.1 are of great significance for the promotion of sustainable urban development. This study employed the spatiotemporal normalized threshold method to extract urban built-up areas in the Pearl River–Xijiang Economic Belt based on night-time light data and investigated the intricate patterns of urban expansion from 2000 to 2020. Then, the historical trends of the SDG11.3.1 indicators within the economic belt were evaluated, and future urban built-up areas were predicted based on the SSP1 scenario. The results indicate the following: (1) Built-up area extraction has an overall accuracy that exceeds 97% and G-mean values that all surpass 82%, indicating the high accuracy of the method. (2) The Pearl River–Xijiang Economic Belt demonstrates evident urban expansion trends, albeit with uneven development. The urban area of the economic belt has expanded from 1020.29 km2 to 3826.87 km2, the expansion direction of each city is different, and the center of gravity of the economic belt has moved to the southeast. (3) During the period from 2008 to 2020, the entire economic belt experienced a situation where the urban expansion rate was lower than the population growth rate, and there was an imbalance in urban development (LCRPGR = 0.33). However, looking ahead to the period from 2020 to 2030, the average LCRPGR for the entire economic belt shows a significant upward trend, approaching the ideal state of sustainable development (LCRPGR ≈ 1).