bluuebunny/crossref_metadata_2025 huggingface dataset:
Educação e Relações Interprofissionais na Saúde: Uma Revisão Integrativa
Authors: Schatz Kwiatkowiski Heloísa , Heinz Marina Klein , Schneider Larissa Gabriella , Silva Carlos André Guerreiro , Da Silva Ana Júlia Sandri , Zanotelli Silvana dos Santos , Resende e Silva Débora Tavares
Published: May 2022

Objetivo: analisar a produção nacional existente sobre educação e relações interprofissionais. Metodologia: constitui-se um estudo bibliográfico, do tipo revisão integrativa, utilizando a Biblioteca Virtual em Saúde. Os critérios utilizados para inclusão foram trabalhos disponíveis de forma completa, produções brasileiras, no idioma português, espanhol e inglês publicados entre o ano de 2015 e 2020. Foram critérios de exclusão artigos duplicados e que a temática não estivesse relacionada ao interesse da pesquisa. Síntese dos dados: percebe-se que a educação interprofissional está em consonância com os princípios e diretrizes do Sistema Único de Saúde e vem sendo incorporada no Brasil há alguns anos. Destaca-se a importância das políticas e Instituições de Ensino Superior para o desenvolvimento de profissionais colaborativos interprofissionalmente. Ainda, identifica-se a positividade percebida em relação à integração da interprofissionalidade no ensino, bem como também desafios para sua efetiva incorporação, tanto no ensino, quanto nos ambientes de trabalho. Conclusões: este estudo (re)afirma a importância da temática pesquisada, tanto na formação em saúde, como na atuação profissional, contribuindo, sobremaneira, para a melhoria da atenção à saúde no contexto dos serviços de saúde, atualmente.
Du Côté de La Côte, um filme sobre turismo
Authors: Sanchez Edney , Brufato Aline
Published: May 2022

Às vezes nos deparamos com um filme que nos surpreende. Du Côté de La Côte (1958) de Agnès Varda é um desses filmes. Curta-metragem documental sobre a Riviera Francesa e seus turistas no verão, encomendado pelo Office National du Tourisme para divulgar a Côte D’Azur, destino turístico na França conhecido no mundo todo....
Editorial – An Alternate Technique for Goniotomy
Authors: Nouri-Mahdavi Kouros
Published: May 2022

This is an Editorial and does not have an abstract. Please download the PDF or view the article HTML.
Letter – Noninvasive Markers of Arteritic Anterior Ischemic Optic Neuropathy in Giant Cell Arteritis
Authors: Bajpai Vidhi , Madan Siddharth , Rana Gunjan
Published: May 2022

This is a Letter to the Editor and does not have an abstract. Please download the PDF or view the article HTML.
Erratum – Carrier Status for p.Gly61Glu and p.Arg368His CYP1B1 Mutations Causing Primary Congenital Glaucoma in Iran
Authors: Heshmati Ali , Taghizadeh Peyman , Ahmadieh Hamid , Yaseri Mehdi , Suri Fatemeh , Alizadeh Mahsa , Dadashzadeh Marjan , Khatami Hajar , Moradkhah Navi Monireh , Zamanparvar Parisa , Behboudi Hassan , Elahi Elahe
Published: May 2022

This is an Erratum to "Carrier Status for p.Gly61Glu and p.Arg368His CYP1B1 Mutations Causing Primary Congenital Glaucoma in Iran" [J Ophthalmic Vis Res 2021;16(4):574–581] and does not have an abstract. Please download the PDF or view the article HTML.
Thermal and Fluid Flow Performance Analysis of Tubular Microchannel Heat Sinks with Inward Protrusions and Nanofluids
Authors: Fetuga Ibrahim Ademola , Olakoyejo Olabode Thomas , Siqueira Antônio Marcos de Oliveira , Gbegudu Joshua Kolawole , Adeyemi Ebenezer Aderibigbe
Published: May 2022

In this work, the application of protrusions and nanofluids to improve the performance of tubular-microchannel heat sink (MCHS) is proposed and investigated computationally. The three-dimensional Navier-Stokes and energy equations were solved numerically using the finite volume method incorporated into the ANSYS (Fluent) software package. The effects of different types of nanofluid (Al2O3, CuO, ZnO in pure water), the volume fraction of the nanoparticles (0% to 4%)  and height of the protrusion ( 2um-6um) on microchannel heat sinks were investigated under the steady-state condition and Reynold numbers (400-2000) with constant heat flux of 9 x 106 W/m2. It was revealed that thermal performance improved as protrusion height increased. At Re=2 000  , for Al2O3 nanofluid (NAN) with a volume fraction ( of 4% and a protrusion height (H)  of  2um to 6um yielded a thermal performance value of 1.59, 1.68, 1.77, 1.86, and 1.96 times that of MCHS without the protrusion, respectively. In addition, at a volume fraction of 4%, protrusion height of 6um  and Reynolds number of 800, the Al2O3, CuO, and ZnO nanofluids yielded a thermal performance value of 1.79, 1.08, and 1.07 times that of pure water, respectively. Furthermore, at a Reynolds number of 400 and a volume fraction of 4%, the Al2O3–water nanofluid reduced the maximum temperature of the MCHS wall by 4% , whereas - and -nanofluids decreased the MCHS wall maximum temperature by 0.5% and 0.48% when compared to pure water, respectively. However, for all the cases of volume fraction (1% to 4%), there was an increase trend in the value of thermal performance for the Reynolds number range of 400 to 800 , and decrease with the Reynolds number range of 800 to 2 000.
Implementation of the approaches of the "Lean region" in the development of the system for assessing the quality of education of the Khanty-Mansi autonomous okrug – Ugra
Authors: Grosheva Tatiana A. , Diveeva Galina V.
Published: May 2022

Introduction. Leather-made technologies are becoming increasingly distributed not only in the production sector of Russian enterprises, but also in the system of state and municipal management, proving their effectiveness and effectiveness. The article is attempted to summarize the idea of ​​the implementation of leaning production in the regional management system and the rationale for the integrated system of "leaning" management on the example of an education quality assessment system of the Khanty-Mansi Autonomous okrug Ugra.

The purpose: is to analyze the implementation of the concept of a "lean region" in the quality management system of education of the of the Khanty-Mansi Autonomous okrug Ugra.

Methods: The article uses such general scientific research methods as a generalization, analysis and synthesis, concretization.

The results are determined by the main directions for the implementation of the concept of "leaning region" in the system of government quality management of the provision of services of the social sphere. The regional methodology for a comprehensive assessment of the quality of educational results of students of the Khanty-Mansi Autonomous okrug Ugra is analyzed from the position of the implementation of the proceeding principles identified at theoretical stage.

The conclusions by the author substantiates the need for a comprehensive approach to the implementation of leaning technologies into the system of social services quality management, including such components such as an increase in the rate of information processing, eliminating a number of "typical" losses, management on the principles of "continuous improvement systems", as well as the development of "foresight systems.
The system of punishments in modern French criminal law
Authors: Podroykina Inna A.
Published: May 2022

The purpose of the study is to analyze the criminal legislation of France from the point of view of possible borrowing of experience to improve the domestic system of criminal penalties.
The work is based on the method of system analysis, and along with it also formal-legal, comparative-legal and concrete sociological methods.
Results: The article expresses the position that the process of building a system of criminal penalties in Russia cannot be considered complete, since from the entire list of penalties enshrined in Art. 44 of the Criminal Code of the Russian Federation, and numbering 13 types of punishments, only some of them turned out to be in demand in practice, such as a fine, compulsory and corrective labor, deprivation of the right to hold certain positions or engage in certain activities, and imprisonment. According to the author, the construction of an effective system of punishments in Russia is impossible without referring to foreign experience, in connection with which he analyzes the French criminal law in terms of determining the system of punishments.
Conclusions: Based on the analysis, the article concludes that a number of positive aspects related to the regulation of the system of punishments under the French Criminal Code can be taken into account by the Russian legislator in the further improvement of the domestic system of criminal penalties.
Resource approach to the professional well-being of teachers in the conditions of the Far North
Authors: Zhuravleva Yulia A.
Published: May 2022

The purpose of the research presented in the article was the theoretical analysis of scientific papers devoted to the problem of studying the unfavorable factors of professional activity of teachers in the conditions of the Far North of Russia. It is shown that the combination of unfavorable climatic, geographical and socio-psychological factors have a destructive effect on the well-being of teachers and are often experienced by them as critical situations. The results of a theoretical and empirical study of teachers' experiences of difficult, critical situations allowed us to determine individual psychological characteristics that make up the teacher's personality resource. The idea of teachers' personal resources, including the severity of temperament properties (activity, pace, emotionality, impulsivity restraint, plasticity rigidity), the features of the dominant mental state and mood from the position of their favorability or unfavorability, the level of psychological stability of the individual formed the basis of the author's model of prevention. The developed model of psychological and pedagogical prevention can be used by educational psychologists as a theoretical and methodological basis for the development of work programs with teachers in educational organizations of the Far North of Russia.
Development of a recursive model for the calculation of transient processes in electric networks using the wavelet transformation
Authors: Dolgikh Nadezhda N. , Dyuba Elena A. , Osipov Dmitry S.
Published: May 2022

The digital transformation of the electric power industry is one of the priority tasks for the development of the industry. Wavelet transform is widely used in the electric power industry to analyze the dynamics of complex non-linear non-stationary processes. The article proposes a method for calculating transient processes in electrical networks based on a recursive algorithm. The approximating and detailing wavelet coefficients of the discrete wavelet transform are used as the voltage signal. To select the optimal wavelet function, it is proposed to use a criterion that takes into account the accuracy of the signal recovery as a result of the inverse wavelet transform. The nature of the change in the calculation result with an increase in the number of iterations is shown. The results of a numerical experiment for a 110 kV network when calculating a three-phase short circuit showed an acceptable accuracy of the developed technique. The proposed technique makes it possible to compress the volume of transmitted digital data on normal and emergency modes of electrical networks.
Development of a method for analysis and forecast of the quality of electric power microgrid in the Far North
Authors: Kovalev Vladimir Z. , Shcherbakov Alexander G. , Khamitov Rustam N.
Published: May 2022

The article is devoted to the construction of an algorithm for predicting and evaluating the quality of MicroGrid electric power in the Far North. A fragment of the daily schedule of the electrical load of a small settlement in the Far North is given. The system features of electrical energy conversion in isolated power supply systems are shown. A structural model of the MicroGrid power supply system of the Far North has been built. A computational experiment was carried out to assess the quality of electrical energy MicroGrid in the Far North. The potential area for the output of electric energy quality indicators beyond the permissible limits according to GOST 32144 2013 is determined.
Calidad percibida en karaokes de Lima Moderna
Authors:   , Carhuavilca Palomino Liz Sarita , Nole Grados Geraldine Fiorella
Published: May 2022

El objetivo del presente trabajo de investigación es determinar la calidad percibida en los servicios de karaokes de Lima Moderna. Para dicha investigación se utilizó la metodología del modelo SERVQUAL. El diseño de la investigación es un estudio descriptivo, cuantitativo y no experimental y de tipo aplicado. Se realizaron 233 encuestas digitales enviadas por conveniencia a clientes que asisten frecuentemente a los principales karaokes ubicados en los diversos distritos de Lima Moderna. Las preguntas se agruparon en cinco factores; tangibilidad, fiabilidad, capacidad de respuesta, seguridad y empatía. Los resultados evidencian la validez del instrumento utilizado para la investigación con una fiabilidad de 0,97. Como resultado del estudio de investigación, se concluye que son los factores de fiabilidad y seguridad los que influyen significativamente en la calidad percibida por los clientes del servicio de karaokes. El trabajo también investiga la relación entre factores, atributos y la calidad percibida por rangos de edad, así como comparativos entre principales distritos y karaokes de Lima Moderna. En el Perú no existen estudios que aplican el modelo SERVQUAL para medir la calidad de servicio en los servicios de entretenimiento denominados karaokes, por lo que, creemos, esta investigación servirá de base para futuros estudios sobre este rubro de negocio en Lima Moderna.
A Short-Term Intercultural Immersion Program in the US: Contextualizing Intercultural Adjustment Journeys
Authors:   , Melchor Bazalar Ariana
Published: May 2022

La educación superior ha sido impactada de gran manera por un mundo crecientemente interconectado y las demandas de empleadores por graduados con educación internacional, catalizando una tendencia de programas study-abroad de corta duración. A pesar de su crecimiento prominente y la expansión de su alcance, surgen desafíos clave porque la investigación sobre los aprendizajes de los estudiantes y la efectividad de estos programas aún se encuentra en etapas iniciales. Es fundamental entender los study-abroad como una variable cambiante, en donde el diseño del programa se relaciona con los aprendizajes del estudiante y las experiencias específicas de ajuste intercultural estén enmarcadas en su contexto. El Iacocca Global Village en Lehigh University (EE. UU.) fue elegido como estudio de caso. Los hallazgos muestran que se necesita una pedagogía intencional enfocada en resultados interculturales respaldada por tutorías continuas. Es importante tener una participación obligatoria en integración cultural y grupos de apoyo, la presión por el trabajo real y la responsabilidad es un requisito previo para dejar la zona de confort y fomentar los encuentros interculturales y que las redes de egresados ​​puedan contribuir a mantener el sentido de pertenencia desarrollado en el programa.
Propiedades psicométricas del Cuestionario de Violencia entre Novios universitarios
Authors:   , Raiser Velarde Romina Esperanza
Published: May 2022

El objetivo del presente estudio fue determinar las propiedades psicométricas del Cuestionario de Violencia entre Novios (Rodríguez, López, Rodríguez, Bringas & Antuña, 2010) en universitarios de la provincia de Tacna, Perú. La muestra estuvo compuesta por 500 estudiantes de una universidad privada, con edades entre 18 a 30 años. Se aplicó el Cuestionario de Violencia entre Novios y la Escala de Violencia contra las mujeres en relaciones de pareja (Vara & López, 2016). Para evaluar la evidencia de validez de la estructura interna del instrumento, se realizó un análisis factorial confirmatorio, el cual dio como resultado como resultado adecuado índices de ajuste para el modelo de ocho factores relacionados (χ²/gl = 4.574, CFI = .961, TLI = .956 SRMR = .054, RMSEA = .085). Se calculó la consistencia interna y se obtuvieron valores alfa y omega que oscilan entre .84 y .91. Se utilizó la prueba no paramétrica de Spearman para el análisis de validez convergente. El análisis de correlaciones entre factores del modelo planteado y la escala de VcM dio como resultado que todas son positivas y estadísticamente significativas (p < .05), con magnitud alta (rs > .50) y moderada (rs > .30). Se concluye que el CUVINO cuenta con adecuadas propiedades psicométricas para su uso en la muestra de estudio.
Vínculo afectivo de pareja en adultos con ausencia paterna en la infancia
Authors:   , Bozzo Uyén Paula
Published: May 2022

El objetivo del estudio es describir la manera en la que se manifiesta el vínculo afectivo de parejas en adultos con ausencia paterna biológica en la infancia. Desde el paradigma cualitativo, se utilizó el diseño fenomenológico, usando como técnica de recolección de información la entrevista semiestructurada. Participaron 9 jóvenes entre hombres y mujeres comprendidos entre 22 y 26 años, que tienen o han tenido una relación de pareja. Se utilizó el análisis contenido obteniendo como categorías: Contextualización, Intimidad y Calidad de la relación analizando lo expresado por cada participante. Se encontró que los participantes presentan dificultades para relacionarse en el plano amoroso, expresar sus emociones, comunicarse; así mismo mencionan tener poco apoyo por parte de sus parejas, inseguridad y baja autoestima
Seeing the Combined Effects of Aid Programmes
Authors: Burge Richard , Nadelman Rachel , McGee Rosie , Fox Jonathan , Anderson Colin
Published: May 2022

Multiple aid agencies often try to support change in the same places, at the same time, and with similar actors. Surprisingly, their interactions and combined effects are rarely explored. This Policy Briefing describes findings from research conducted on recent aid programmes that overlapped in Mozambique, Nigeria, and Pakistan, and from a webinar with UK Foreign, Commonwealth & Development Office (FCDO) advisors and practitioners. The research found three distinct categories of ‘interaction effects’: synergy, parallel play, and disconnect. We explore how using an ‘interaction effects’ lens in practice could inform aid agency strategies and programming.
Szaleństwem jest marzyć o społeczeństwie szczęśliwym… „Записки українського самашедшого” Liny Kostenko i „W roku 2000. (Z przeszłości 2000–1887)” Edwarda Bellamy’ego
Authors: Jakubowska-Krawczyk Katarzyna
Published: May 2022

The article reflects on dreams about social life, disappointments and finally the belief in breakthrough moments. Two novels, Zapysky ukrainskoho samashedshoho by Lina Kostenko and Looking Backward: 2000–1887 by Edward Bellamy were used as research material. I based my analysis on the sociological concepts of the lifeworld and the theory of transformative learning. I showed that the subject of both novels was not only the critique of contemporary society and political life, but, most of all, the weakness of interpersonal relations. Thus, both of these novels not only are narratives about a specific social, political or ideological situation, but also acquire a universal dimension.
Between poetology and nation building: complex identities in Ukrainian underground poetry of the 1960s–1980s
Authors: Achilli Alessandro
Published: May 2022

In this essay, I analyse how identities are constructed in Ukrainian underground poetry of the late Soviet period. On the basis of selected poems by Mykhailo Hryhoriv and Vasyl’ Stus, I explore the complex dynamics between the explicit commitment to fostering national identity that is often regarded as a standard duty of Ukrainian writers and the pursuit of a transnational, purely literary identity as part of a wider embrace of the modernist imagination.
Apresentação
Authors: Kopelman Isa Etel , Possani Maria Alice
Published: May 2022

Apresentação do volume 12, ano de 2022
The history of the library of Rzhanov Institute of Semiconductor Physics of the Siberian Branch of the Russian Academy of Sciences (to the 65th anniversary of the library)
Authors: Shaburova N. N.
Published: May 2022

The history of foundation and development of one of libraries of the Siberian Branch of the Russian Academy of Sciences (SB RAS) – the Institute of Semiconductor Physics is briefly presented, interesting facts are given, and the types of its activities at the present stage are described. The object is to acquaint the library community with the work of the academic library staff in its historical aspect and to show the need for scientific and methodological support for its full-fledged activities.
Post-General Anesthesia Angioedema After General Anesthesia
Authors: Panggabean Mathilda , Solihat Yutu
Published: May 2022

Background: General anesthesia includes the administration of avariety medicines to patient/in/ fairly short/period. Numerous negative consequences can occur because of reaction from the drugs given, one example is anaphylaxis that may lead to death if not been recognized and handled quickly & promptly.
Case Illustration: A sixty-seven years old male patient 152 cm 65 kg with multiple cholecystitis and obstructive jaundice, who was scheduled to undergo laparoscopic cholecystectomy. Patient had no records of allergy to medication and food. Family has no records to hypersensitive reactions or angioedema. At initial evaluation, blood pressure was 190/90 mmHg and hemoglobin 10,4 g/dL while the rest parameter was within normal limits. We given propofol and rocuronium to induce relaxation and facilitate intubation in surgery. Approximately 90 minutes after rocuronium injection, patient evolved angioedema across the face/area, tongue, and floor of the mouth. Patient also complained of not being able to talk. Soon after that, we give 10 mg of dexamethasone and 100 mg hydrocortisone to the patient. Within 60 minutes after hydrocortisone administration the patient shows/clinical improvement that marked by swelling began to decrease and the patient was able to speak again. Then we educate patient about angioedema and its alert.

Conclusion: Anaphylaxis is a type of hypersensitivity response that take place to a particular allergen and mostly self-limited, but 11% of hypersensitive reaction require airway intervention. Most of anesthetic medication such as thiopental sodium, propofol, muscle relaxants, and etomidate may induce anaphylactic reaction during anesthesia induction. Discontinuation of underlying agent and airway control may prevent mortality and morbidity.
Traumatic Cervical Facet Fractures and Dislocations
Authors: McDonald Christopher L. , Daniels Alan H. , Anderson George A. , Alsoof Daniel , Kuris Eren O.
Published: May 2022

» Flexion-distraction, axial loading, and rotational forces can cause various degrees of osseoligamentous disruption of the cervical spine, leading to traumatic cervical facet fractures and dislocations.

» Low-energy forces lead to minimally displaced facet fractures that often can be treated with immobilization only. High-energy forces are more likely to cause unstable injuries with or without neurologic compromise, which may require surgical intervention.

» The initial treatment of cervical facet injuries requires patient evaluation and management through the Advanced Trauma Life Support (ATLS) protocols, while definitive management varies based on the biomechanical components of the injury, the neurologic status of the patient, and additional patient factors.

» Cervical facet injuries often require a multidisciplinary approach to optimize long-term functional outcomes and minimize serious complications.
Digitalization Of Tax Administration Systems And Tax Complience Costs On Taxpayer Complience Of Individual Taxpayer
Authors: Wulandari Dian Sulistyorini
Published: May 2022

This study aims to examine the effect of the application of e-filing, e-billing, e-invoicing, and tax compliance costs on taxpayer compliance. The independent variable in this research are e-filing, e-billing, e-invoicing, and tax compliance, while dependent variabe is taxpayer compliance. The population in this research was an individual taxpayer listed in KPP Pratama South Cikarang. The sample in this study is determined by random sampling method with a sample size of 100 respondents. the primary data collectin method used in this study is a survey method with questionnaire media. The method of analysis used is multiple linear regression. The results of this research is shows that the effect  of the application of e-filling and e-invoicing has a positif effect on taxpayer compliance. Tax compliance cost have a negative effect on taxpayer compliance. While the effect of the application of the e-billing has no effect on taxpayer compliance.
Rumen microbiota alterations during subclinical ketosis is associated with the development of subclinical mastitis in dairy cows
Authors: Xiang Kaihe , Shen Peng , Mu Ruiying , Li Shuang , Wang Ying , Zhao Caijun , Zhang Naisheng , Hu Xiaoyu , Fu Yunhe
Published: May 2022

Background: Mastitis is the most serious disease endangering animal husbandry, especially dairy farming. Clinical investigations indicated that cows suffering from ketosis have a higher probability of mastitis. The composition of rumen microbiota is closely related to ruminant health. However, it is not clear what role it plays in this process.Results: Blood β-hydroxybutyrate (BHBA), blood non-esterified fatty acids (NEFA), milk somatic cell counts (SCC), milk fat, fat to protein ratio, and milk urea nitrogen (MUN) were significantly elevated in all cows with subclinical ketosis (SCK)，while the milk lactose was significantly decreased. The production and proportion of volatile fatty acids (VFA) in rumen changed during SCK, with significant decreases in acetate, propionate, butyrate, and total VFA (TVFA), and a significant increase in the acetate to propionate ratio. The microbiota in rumen fluid and milk were determined by 16S rRNA gene sequencing. The results showed that the richness of rumen microflora was significantly decreased during SCK. The rumen harbored unique bacterial community in cows suffering SCK, which were significantly correlated with BHBA in plasma, SCC in milk and milk components. LEfSe analysis revealed that it was characterized by the increase of Bacteroidota, Christensenellaceae_R-7_group, Luteimonas and Thermomonas and the decrease of Prevotella. The richness of milk microflora was significantly increased during SCK. LEfSe analysis suggested a significant increase in Staphylococcus and a significant decrease in Coxiella, Acinetobacter, Oscillospiraceae_UCG-005 and Corynebacterium. Conclusions: The SCC of cows with subclinical ketosis was significantly elevated and all individuals suffered from subclinical mastitis. The rumen microbiota during subclinical ketosis changed significantly, characterized by decreased richness, increased acetate and butyrate producing bacteria and decreased propionate producing bacteria, and was closely related to blood BHBA and milk SCC. This provides a new perspective for the study of the relationship between subclinical ketosis and subclinical mastitis as well as the prevention and treatment of the two diseases.
Immediate Effect of Trunk Flexion and Extension Isometric Exercise Using an External Compression Device on Electromyography of the Hip Extensor and Trunk Range of Motion of Healthy Subjects
Authors: Huang Tian-zong , Kim Suhn-yeop
Published: May 2022

Background: Sacroiliac joints (SIJs) transmitted trunk load to lower extremities through the lumbopelvic. External compression devices across the SIJs could provide stability to the SIJs. A previous study established that using a device known as Active Therapeutic Movement version 2 (ATM®2) has been developed to improve pain and joint range of motion (ROM) in patients with LBP. However, no study has examined the physiological change in the muscle through ATM®2-based exercise thus far. This study aimed to determine the immediate effects of ATM®2 exercise on the contraction timing, back extension endurance, muscle fatigue, and trunk ROM of lumbar and lower limb muscles in healthy subjects.Methods: Thirty-six healthy subjects (mean age=23.16±2.3) volunteered to participate in this study. Subjects were instructed to perform ROM test using sit and reach test, back extensor endurance test using Biering-Sorensen test, erector spinae (ES), lumbar multifidus (LM) fatigue and onset time of Gluteus maximus (GM) in prone hip extension using electromyography before and after trunk flexion and extension isometric exercises.Results: The ROM in trunk flexion showed a significant increase of 7.9% after exercise compared to that before exercise (p<0.05). Relative GM contraction onset timing significantly decreased after exercise (p<0.05). The result of the Sorensen test after exercise showed a trend of increase in duration time. Muscle fatigue in the LM, however, showed a significant increase (p<0.05), whereas muscle fatigue in the ES was reduced without statistical significance.Conclusions: The results base on this study showed a significant increase in the trunk ROM after trunk flexion and extension isometric exercise using an external compression device, while the relative contraction onset timing in the GM was significantly reduced. Furthermore, the muscle endurance test after exercise showed a trend of increase in the duration time with a decreasing trend in muscle fatigue in the ES. Exercise-based on ATM®2 is an effective exercise protocol with an effect on the biomechanics of healthy subjects.· Clinical trial registration numbers : KCT0006728· Clinical trial registration date : 09/11/2021
Cold stress induces colitis-like phenotypes in mice by altering gut microbiota and metabolites
Authors: Sun Lijuan , Wang Xueying , Chen Yue , Song Jianxia , Liang Changting , Zhang Jianbin
Published: May 2022

Objective
The westernized lifestyle has been paralleled by an epidemic of inflammatory bowel disease (IBD). Excessive consumption of cold beverages is especially common among the modern humans. However, whether cold stress contributes directly to the gut barrier and gut-brain axis is not clear. We investigated whether the cold stress affect gut inflammation or not.
Material
: 16 male mice were used in this study.
Treatment:
The mice were treated with 14 consecutive days of intragastric cold or common water administration.
Methods
We observed changes in gut transit and gut barrier in the colon.We also employed RNA sequencing-based transcriptomic analysis to identify the genes potentially driving gut injury, and simultaneously examined the gut microbiota and metabolites in the feces.
Results
Cold stress inhibited the colonic motility and increased gut permeability. A set of core genes related to immune responses were consistently overexpressed in the cold stress group. Additionally, cold stress induced decreased bacterial diversity, ecological network, and increased pathogens mainly belonging to Proteobacteria. The dopamine signaling pathway-related metabolites were largely reduced in the cold stress group.
Conclusion
This study revealed that cold stress could trigger an IBD-like phenotype in mice, implying that cold stress is a possible risk factor for IBD development.
Inverse association between fasting blood glucose and occurrence of gallbladder cancer in type 2 diabetes mellitus patients: a case-control study
Authors: Sheng Bin-Wu , Zhang Jianqin , Chen Min , Ma Mao
Published: May 2022

Purpose To explore the reason for the correlation between diabetes mellitus (DM) and gallbladder cancer (GBC) in an epidemiological setting. Methods We summarized the clinical and laboratory data of 2210 GBC patients within 3524 Chinese patients in our hospital from January 2009 to December 2020. Seventeen influencing factors for GBC, including gender, body mass index (BMI), fasting blood glucose (FBG), fasting insulin (FINS), homeostasis model assessment of insulin resistance (HOMA-IR), retinol binding protein (RBP), lipid indexes, and others, were analyzed by unconditional logistic regression analysis in this case-control study. Results On univariate logistic regression, GBC likelihood was significantly and positively correlated with serum triglyceride, low-density lipoprotein cholesterol, FINS, and HOMA-IR; being female; BMI; DM; NAFLD; and GSD, and it was significantly negatively correlated with high-density lipoprotein cholesterol and FBG concentrations in serum and with hypertension. In multivariate analysis, FINS was significantly positively associated with GBC risk, while DM had an insignificant negative association, and FBG levels were also not important. HOMA-IR was a most remarkable independent factor of GBC risk in DM patients. FBG levels showed a significant negative relationship to GBC in DM individuals. Conclusions Our findings revealed that the efficient treatment of insulin-resistance is an important approach for decreasing GBC risk, as opposed to lowering blood sugar alone, especially in DM patients. Interestingly, FBG might have an inverse association with the development of GBC in type 2 DM patients. Exciting, we found initially that a dramatic drop in RBP might be helpful in predicting the occurrence of GBC.
SSR mining of black cumin (Nigella sativa L) transcriptome for molecular markers development
Authors: Celik Ibrahim , AYDIN Adnan
Published: May 2022

Black cumin (Nigella sativa L.) is an important aromatic plant due to the production of many health-beneficial metabolites. Up to date- molecular characterizations of the germplasm were performed by using general marker systems. In the present study, the transcriptome of the black cumin was searched for SSRs. Dinucleotide and trinucleotide repeats were the major repeat types which comprised 52.4% and 44.7% of total SSRs, respectively. TC/GA was the most frequent repeat motif (11.8%). A total of 4,779 SSR markers were developed. Functional annotation of 2,456 SSR markers was performed. For validation of the SSR markers, randomly selected 20 markers were tested in a subset population containing nine Nigella sativa and one Nigella damascena genotypes. A total of 13 SSR makers produced clear amplification and generated 41 polymorphic fragments with mean gene diversity (0.25). Principal Coordinate Analyses (PCoA) and MCMC methods based on hierarchical clustering analysis revealed two clusters and moderate diversity with a mean value of 0.66 and 0.57 for interspecific and intraspecific populations, respectively. This is the first report of the development of SSR markers from black cumin transcriptome. These high-throughput crop-specific SSR markers are valuable genomic sources for comprehensive genome analysis in black cumin.
Upregulated ONECUT2 impeded the efficacy of 5-FU treatment to relapsed patients and might be an emerging indicator of chemotherapy in gastric cancer
Authors: Zhu Haifeng , Chen Shu , Long Weiguo , Wang Xu
Published: May 2022

Background: Chemotherapeutic resistance is a main obstacle for prolonging the lifetime of gastric cancer (GC) patients, so new emerging solutions still remain to be investigated. We aim to identify an effective target, ONECTU2, for chemotherapy of GC patient, particularly relapsed patients.Methods: STAD patients matched with clinical features and transcriptome sequencing data from TCGA were used in this study. Gene set enrichment analyses (GSEA) were performed using 100 patients who were divided average according to ONECUT2 level. Cytotoxic assays for 5-FU and cisplatin were used to test the role of chemotherapeutic resistance of ONECUT2 in GC cells. 30 relapsed patients with previously resected tumor samples were collected to validate the efficacy of ONECUT2 in GC chemotherapy.Results: ONECUT2 was a crucial regulator of Disease-Free Interval (DFI) in GC patients with reoccurrence once having chemotherapeutic treatment. Gene set enrichment analysis (GSEA) results suggest that upregulated ONECUT2 is positively correlated with the increased ability of xenobiotic drug metabolism, which might contribute to the pharmaceutical resistance in GC. Targeting ONECUT2 reduced half-maximal inhibitory concentration (IC50) of 5-FU and cisplatin in GC cells. The efficacy of 5-FU treatment was negatively correlated with ONECUT2 mRNA level of tumor samples derived from GC patients who relapsed after surgical resection.Conclusions: These findings indicate that ONECUT2 displays a drug-resistant function in GC, providing a new therapeutic target for GC treatment.
Droplet microfluidics based combinatorial indexing for massive-scale 5′-end single-cell RNA sequencing
Authors: Jiang Lan , Li Yun , Huang Zheng , Zhang Zhaojun , Wang Qifei , Fang Xiangdong , Wang Shufang , Li Fengxian
Published: May 2022

Single-cell RNA sequencing methods focus on 5’-end of transcripts can reveal promoter and enhancer activity and allows efficiently profiling immune receptor repertoire. However, ultra-high-throughput 5′-end single-cell RNA sequencing methods have not been described. Here, we introduce five-prime end single-cell combinatorial indexing RNA-Seq (FIPRESCI), enabling the massive sample multiplexing and increasing the throughput of the droplet-microfluidics system by over 10-fold. FIPRESCI is based on combining the ability of indexed Tn5 transposome to barcoding RNA/cDNA hybrids heteroduplexes in situ and Template Switching Oligo barcoding of commercial droplet-microfluidic. Using FIPRESCI, we profiled transcriptome and transcribed cis-regulatory elements from various human and mouse cell lines and demonstrated the approach is compatible for both cells and nuclei. We applied FIPRESCI to E10.5 whole mouse embyro and uncover previously unknown isoform switch during GABAergic neurogenesis of several important regulators, including Rbfox2. We further applied FIPRESCI to primary T cells from human peripheral blood mononuclear cells (PBMCs) and demonstrated it enable simultaneous identification of cancer specific gene expression and T cell receptor (TCR) signatures. Given its simplicity, flexibility, and scalability, FIPRESCI will have wide application in cell atlas projects, large-scale screening, and single-cell immune profiling of large cohorts’s studies.
Invading bivalves replaced the native Levantine populations with negligible local effects on the benthic community
Authors: Diga Rei , Gilboa Merav , Moskovich Raz , Darmon Neomie , Amit Tal , Belmaker Jonathan , Yahel Gitai
Published: May 2022

Constructing the Suez Canal connected the Red Sea and the Mediterranean Sea, allowing rapid marine bio-invasion. Over the last century, several bivalve species have invaded the Levantine basin, yet their distribution and impact on the benthic community have not been thoroughly studied. Large-scale benthic surveys along the rocky substrate of the Israeli Mediterranean coastline indicate that invading bivalves now dominate the rocky environment, reaching densities of tens to hundreds of individuals per m2. No native bivalve specimens were found in any of the transects surveyed. The small-scale ecological effects of the established invading populations on the benthic community were examined using in-situ exclusion experiment where all invading bivalves were either physically removed or poisoned and kept in place to maintain the physical effect of the shells. Surprisingly, the experimental exclusion showed little measurable effect of bivalve presence on the invertebrate community in close vicinity (~1 m). Bivalves presence had a small, but statistically significant, effect only on the community composition of macroalgae, increasing the abundance of some filamentous macroalgae, and reducing the cover of turf. The generally low impact of invading bivalves could be due to (1) wave activity and local currents dispersing the bivalve excreta, (2) high grazing pressure, possibly by invading herbivorous fish, reducing the bottom-up effect of increased nutrient input by the bivalves, or (3) the natural complexity of the rocky habitat masking the contribution of the increased complexity associated with the bivalve’s shell. We found that established invading bivalves have replaced native bivalve species, yet their small-scale effects on the benthic community seem to be negligible.
Small and large scale engineering design optimization using an innovative hybrid algorithm
Authors: Parouha Raghav Prasad
Published: May 2022

An innovative hybrid algorithm (ihPSODE) present in this paper, for finding the solution of small and large scale engineering design optimization problems. A new particle swarm optimization (nPSO) and differential evolution (nDE) introduced in ihPSODE, to promote the search accuracy. Besides, a novel inertia weight, acceleration factor and position update structure is adopted in nPSO to increase the population diversity as well as a novel mutation approach and crossover rate is implemented in nDE to escape from local optima. After fitness function calculations recognize the top half member with discard rest half and apply nPSO which help to sustain exploration and exploitation competency of the algorithm. Furthermore, to achieve rapid convergence and stability, apply nDE on offspring created by nPSO. The population resultant by nPSO and nDE are combined for repetition. The efficiency of ihPSODE with its integrating nPSO and nDE algorithm validated on 23 unconstrained benchmark functions and utilized to solve 5 small (structural optimization) and 1large (economic dispatch with 3-, 6-, 15-, 40-, 140-unit test system considering with or without valve point effect) scale engineering design optimization problems plus comparing the results with various modern algorithms. Experimental and equated results confirm the superiority of the proposed algorithms.
Evaluation of Gentamicin Dosage Regimen and Route of Administration Based on Achieved Serum Concentrations in Neonates
Authors: Lim Chuan Poh , Candra Samuel Rocky , Edison Ebenezer Priyantha , Tan Mary Grace , Yong Mei Hui Amanda , Chen Yufei , Yeo Cheo Lian
Published: May 2022

Purpose
Combination antibiotics consisting beta-lactam and aminoglycoside are commonly utilized in the treatment of neonatal septicaemia. The aims of this study were (1) to determine safety and efficacy of the revised gentamicin dosage regimen and (2) to compare intravascular (IV) versus intramuscular (IM) route of gentamicin administration, in term of attaining peak and trough serum concentration targets.
Methods
This was a retrospective study from 2012 to 2017. All neonates who received gentamicin with therapeutic drug monitoring performed were included in this study. Data for all eligible neonates were collected from electronic medical records and included demographics, serum creatinine levels, the complete gentamicin dosing and concentration history.
Results
A total of 737 neonates who received the correct institutional gentamicin dosage regimen (± 5% of the intended dose) were included in this study. There were 635 trough concentrations (within 30 minutes prior to subsequent dose) and 430 peak concentrations (60 to 90 minutes post administration) included in the comparison of IV and IM route of administration. With the revised dosage regimen, 91% of peak and 98% of trough concentrations were found within therapeutic range. There was no difference in terms of proportion of trough concentrations within therapeutic range for IV as compared to IM (97.8 vs 98.6%, p = 0.556) while IM resulted in a higher proportion of peak concentrations within therapeutic range (86.1 vs 97.0%, p < 0.001).
Conclusions
The current institutional gentamicin dosage regimen is safe and effective in attaining therapeutic targets. Intramuscular injection can be an alternative route of administration for gentamicin in neonates.
Determinants of Nonrenewal of National Health Insurance (NHI) Membership Cards Among Healthcare Workers in the Kintampo North Municipality, Bono East Region of Ghana.
Authors: Hallidu Mustapha , Sumaila Issah
Published: May 2022

Introduction:
National Health Insurance Scheme (NHIS) was implemented in Ghana in 2004 to serve as the lifeline to realizing Universal Health Coverage (UHC). Available evidence suggests that, formal sector workers do not promptly renew their expired NHIS membership cards. This study was therefore conducted to unearth factors responsible for the failure of healthcare workers in the Kintampo North Municipality to promptly renew their health insurance membership whenever it expires.
Methods
A descriptive cross-sectional approach was used to conduct this study where three hundred and ninety-seven (397) participants were recruited using a proportionate stratified sampling technique. All variables with a p-value < 0.25 at the bivariate analysis level were selected and put into multiple logistic regression analysis models for statistical significance (p-value < 0.05). Odds ratios with their corresponding 95% Confidence Interval were reported. A p-value < 0.05 was set as level of significance.
Results
Almost all the respondents (94.0%) had NHIS membership cards; out of which 70.7% had valid membership cards. 40.0% did not renew their expired NHIS membership cards for more than 7 months. From the study, reasons given for health workers’ inability to promptly renew NHIS membership included: 212 (19.8%) indicated forgetfulness, busy schedules 191 (17.9%), procrastination 167 (15.6%), self-medication 170 (15.9%) and utilization of spiritual homes (4.5%). All socio-economic factors were significantly associated at the bivariate level (p < 0.05). However, in the multiple logistic regression model, employment status, the type of health staff and monthly salary lost their statistical significance.
Conclusion
NHIS subscription and membership renewals is high among healthcare works in the Kintampo North District of the Bono East Region of Ghana. However, there is the need to encourage those who do not renew their expired cards by ensuring that more NHIS cards renewal centers are established at close proximity to the health facilities. It will be prudent for NHIS to liaise with Government of Ghana (GoG) to put measures in place to facilitate automatic membership renewals for public sector workers who for some other reasons often fail to renew their cards.