bluuebunny/crossref_metadata_2025 huggingface dataset:
Softwaregestützte Modellierung und Optimierung von Geschäftsmodellen
Authors: Loos Peter , Klein Sabine , Emrich Andreas
Published: September 2019

Unternehmen passen ihre Geschäftsmodelle an, um im Markt bestehen zu können. Grund dafür ist häufig die Digitalisierung von Produkten und Dienstleistungen. Unternehmen können durch die digitale Transformation erheblich profitieren, gleichzeitig werden sie aber auch vor große Herausforderungen gestellt. Geschäftsmodelle helfen ihnen dabei, die Geschäftsideen abzubilden sowie Innovationen zu planen. Es existieren viele Methoden, um Geschäftsmodelle abzubilden, zu optimieren und zu evaluieren, wobei einige dieser Methoden auch in Software-Tools umgesetzt sind. In diesem Beitrag werden bestehende kostenfreie Software- Werkzeuge zur Modellierung von Geschäftsmodellen vorgestellt und deren Nutzen untersucht. Dadurch sollen Anwender befähigt werden, das passende Tool für ihren Geschäftsmodell-Transformationsprozess auszuwählen.
Angebote medienwirtschaftlicher Ausbildung an Universitäten
Authors: Eigler Joachim
Published: September 2019

Dieser Beitrag gibt einen Überblick über medienbezogene betriebswirtschaftliche Studiengänge (Bachelor sowie Master) an Universitäten in Deutschland, Österreich und der Schweiz. Dabei konzentriert er sich zum einen auf diejenigen universitären Bachelor- und Masterstudiengänge, deren Studiengangsbezeichnung die Begriffe „Medienmanagement“, „Medien und Management“ oder „Medienwirtschaft“ beinhaltet. Zum anderen werden Studiengänge berücksichtigt, die einen besonderen und auch vom Gewicht her umfangreicheren Schwerpunkt im Bereich Medienmanagement aufweisen.
Weiterbildung im Medienmanagement: Master, MBA, Zertifikatsprogramme
Authors: Rott Armin
Published: September 2019

Bachelor und was nun? Die Frage stellt sich vielen Absolventen nach Abschluss des Erststudiums. Weiter studieren? Oder erst einmal arbeiten? Und dann vielleicht später noch einen Master draufsatteln? Oder gar keinen Master machen und stattdessen eine Weiterbildung besuchen? Auch Fach- und Personalverantwortliche in Medienunternehmen kennen diese Fragen von ihren Werkstudenten, Mitarbeitern oder sind selbst auf der Suche nach einer systematischen Weiterbildung. Aber welche genau? Welcher Studiengang, welches Programm an welcher Institution mit welchem Abschluss? Akademisch oder praktisch?
Medienmanagement an der FH St. Pölten
Authors: Untersteiner Jutta
Published: September 2019

Ein Fokus der Ausbildung liegt auf der praktischen Medienproduktion. Während des Studiums können Projekte mit Kooperationspartnern aus der Praxis erarbeitet werden, beispielsweise Konzepte für den ORF, die Niederösterreichischen Nachrichten, goTV oder Kronehit.
Tagungsbericht: Medientage Mitteldeutschland 2019
Authors: Reising Wolfgang
Published: September 2019

Durch die Digitalisierung bzw. Vernetzung und eines damit einhergehenden sozialen Wandels fallen nicht nur die sektoralen Grenzen der klassischen Mediengattungen im

Print, Hörfunk und Fernsehbereich, sondern hat das Internet als hybrider Kommunikationsraum neue Formen von Öffentlichkeit entstehen lassen. Für das Medienmanagement klassischer Anbieter, für die national geprägte Gesetzgebung und auch für das Gemeinwohl einer demokratisch, pluralistisch geprägten Gesellschaft stellt dies eine erhebliche Herausforderung dar. Vor diesem Hintergrund standen die Themen Demokratie, Digitalisierung und Journalismus im Mittelpunkt der 2019er Medientage Mitteldeutschland (MTM), die am 21./22. Mai einerseits in einer neuen Umgebung, der Leipziger Baumwollspinnerei, stattfanden und andererseits in den Zeitraum zwischen Abgabe der KEF-Anmeldung der öffentlich-rechtlichen Rundfunkanstalten und der Ministerpräsidentenkonferenz zu Fragen von Auftrag, Struktur und Finanzierung der Öffentlich-Rechtlichen fielen.
Using Radioactive Elements and the Th/U Ratio in Study of the Geochemical Typification of Granitoids and Their Intrusive Character
Authors: Rikhvanov L.P.
Published: September 2019

—This paper considers the use of radioactive elements for the solution of geochemical problems related to granitoid magmatism. The metallogenic types of granites are recognized on the basis of the contents of radioactive (Th-bearing, rare-metal, U-bearing, gold ore, etc.) elements in them. Special attention is focused on the Th/U ratio, which is higher than 2.5 in primary igneous rocks, such as Li–F granites of the Ongon-Hayrhan deposit (Mongolia). Granitoids with Th/U < 2 (and, the more so, Th/U < 1) cannot be considered igneous. They are either metasomatized or of metasomatic genesis. Petrochemical and geochemical data on these types of rocks should be used for petrological implications with great care. Radiogeochemical indices can and must be used as additional indicators of the genesis of rocks, when it is controversial because of the widely manifested convergence and metasomatism processes.
The Olimpiadinskoe Gold Deposit (Yenisei Ridge): Temperature, Pressure, Composition of Ore-Forming Fluids, δ34S in Sulfides, 3He/4He of Fluids, Ar–Ar Age, and Duration of Formation1
Authors: Gibsher N.A. , Tomilenko A.A. , Sazonov A.V. , Bul’bak T.A. , Ryabukha M.A. , Sil’yanov S.A. , Nekrasova N.A. , Khomenko M.O. , Shaparenko E.O.
Published: September 2019

—New thermobarogeochemical and isotope-geochemical data are presented, which show the intricate and long history of the formation of the unique Olimpiadinskoe gold deposit with predicted gold reserves of >1000 tons on the Yenisei Ridge. Metal-bearing oxidized water–carbon dioxide and reduced carbon dioxide–hydrocarbon fluids participated (at the same time or successively) in the formation of the deposit at 220–470 °C and 0.6–2.5 kbar. Fluids of gold-bearing mineral assemblages include CO2, hydrocarbons, and S-, N-, and halogen-containing compounds capable of transporting ore elements, including gold. Highly mobile carbon dioxide–hydrocarbon fluids were responsible for the appearance of disseminated gold mineralization in large bodies of quartz–carbonate–mica schists serving as geochemical barriers in the Olimpiadinskoe deposit. The deposit formed in the period from 817 to 660 Ma, which fits the time interval from crystallization to cooling (868–721 Ma) of the most proximal multiphase Chirimba granitoid pluton. The hydrothermal activity of the fluids that formed the Olimpiadinskoe deposit lasted at least 100–150 Myr year.
Study of Tau Neutrino Production in Proton Nucleus Interactions
Authors: Gornushkin Yu.
Published: September 2019

In the DsTau experiment at the CERN SPS, an independent direct way to study the tau neutrino production in high energy proton-nucleous interactions was proposed. Since the main source of tau neutrinos is a decay of Ds mesons, the project aims at measuring the differential cross-section of this reaction. The experimental method is based on the use of high-resolution emulsion detectors for the efficient registration of events with short-lived particle decays. The motivation of the project, details of the experimental technique, and the first results of the analysis of the data collected during test runs, which prove the feasibility of the study are presented.
Status of the MUSE experiment
Authors: Rostomyan T.
Published: September 2019

The 5.6q difference in proton radii measured with мp atoms and with ep atoms and scattering remains an unexplained puzzle. MUSE will measure the мp and ep elastic scatterings in the same experiment at the same time. The experiment determines cross-sections, two-photon effects, form-factors, and radii and allows мp and ep to be compared with reduced systematic uncertainties. These data should provide the best test of the lepton universality in a scattering experiment to date, about an order of magnitude improvement over previous tests, a 7q radius determination, and improved two-photon measurements.
Regge Cuts and NNLLA BFKL
Authors: Fadin V. S.
Published: September 2019

In the leading and next-to-leading logarithmic approximations, QCD amplitudes with gluon quantum numbers in cross-channels and negative signature have the pole form corresponding to a reggeized gluon. The famous BFKL equation was derived using this form. In the next-to-next-to-leading approximation (NNLLA), the pole form is violated by contributions of Regge cuts. We discuss these contributions and their impact on the derivation of the BFKL equation in the NNLLA.
On the generalization of the Darboux theorem
Authors: Eftekharinasab Kaveh
Published: September 2019

Darboux theorem to more general context of Frechet manifolds we face an obstacle:  in general vector fields do not have local flows. Recently, Fr\'{e}chet geometry has been developed in terms of projective limit of Banach manifolds. In this framework under an appropriate Lipchitz condition The Darboux theorem asserts that a symplectic  manifold $(M^{2n},\omega)$ is locally symplectomorphic to $(R^{2n}, \omega_0)$, where $\omega_0$  is the standard symplectic form on  $R^{2n}$. This theorem was proved by Moser in 1965, the idea of proof, known as the Moser’s trick, works in many situations. The Moser tricks is to construct an appropriate isotopy $ \ff_t $  generated by a time-dependent vector field $ X_t  $ on $M$ such that $ \ff_1^{*} \omega = \omega_0$. Nevertheless,  it was showed by Marsden that Darboux theorem is not valid for weak symplectic Banach manifolds. However, in 1999 Bambusi showed that if we  associate to each point of a Banach manifold a suitable Banach space (classifying space) via a given symplectic form then the Moser trick can be applied to obtain the theorem if the  classifying space does not depend on the point of the manifold and a suitable smoothness condition holds.
 If we want to try to generalize the local flows exist and with some restrictive conditions the Darboux theorem was proved by Kumar.  In this paper we consider the category of so-called bounded Fr\'{e}chet manifolds and prove that in this category vector fields have local flows and following the idea of Bambusi we associate to each point of a manifold a Fr\'{e}chet space independent of the choice of the point and with the assumption of bounded smoothness on vector fields  we prove the Darboux theorem.
How one shall interpret ‘spirit of mutual trust and co-operation’ in NEC contracts
Authors: Leung Raymond H M , Kwok Bryan C H
Published: September 2019

Promoting the ‘spirit of mutual trust and co-operation’ has long been regarded as the ‘pillar’ of NEC contracts, which reflects the recommendation of the Latham report. Undoubtedly, this clause serves as a good catalyst that suppresses adversarial behaviour among parties. Nevertheless, this clause has recently sparked hot debates concerning its legal implication and interpretation. Some commenters claim that the clause refers to good faith obligation, while others suggest that it does not. This paper aims to conduct a review regarding this issue and provides a rough guideline for practitioners.
Kidney Regeneration in Later-Stage Mouse Embryos via Transplanted Renal Progenitor Cells
Authors: Yamanaka Shuichiro , Saito Yatsumu , Fujimoto Toshinari , Takamura Tsuyoshi , Tajiri Susumu , Matsumoto Kei , Yokoo Takashi
Published: September 2019

Significance Statement

Although researchers have attempted to regenerate organs by transplanting stem cells into embryos of another species, there is no established method for transplanting cells into the midstage embryo near the onset of organogenesis or information on kidney regeneration resulting from this approach. The authors describe a method to allow transplantation of renal progenitor cells (embryonic stage–matched cells) into the retroperitoneal cavity of mouse fetuses without lethality. The transplanted cells differentiated to glomeruli with capillary loops (comprising blood vessels from the host) and filtration function. In a mouse model of fetuses with atrophic kidneys, transplanting renal progenitor cells into such fetuses produced a transplanted-cell neonephron. These findings demonstrate potential kidney regeneration through transplanting renal progenitor cells to the site of natural kidney development.

Background

The limited availability of donor kidneys for transplantation has spurred interest in investigating alternative strategies, such as regenerating organs from stem cells transplanted into animal embryos. However, there is no known method for transplanting cells into later-stage embryos, which may be the most suitable host stages for organogenesis, particularly into regions useful for kidney regeneration.

Methods

We demonstrated accurate transplantation of renal progenitor cells expressing green fluorescent protein to the fetal kidney development area by incising the opaque uterine muscle layer but not the transparent amniotic membrane. We allowed renal progenitor cell–transplanted fetuses to develop for 6 days postoperatively before removal for analysis. We also transplanted renal progenitor cells into conditional kidney-deficient mouse embryos. We determined growth and differentiation of transplanted cells in all cases.

Results

Renal progenitor cell transplantation into the retroperitoneal cavity of fetuses at E13–E14 produced transplant-derived, vascularized glomeruli with filtration function and did not affect fetal growth or survival. Cells transplanted to the nephrogenic zone produced a chimera in the cap mesenchyme of donor and host nephron progenitor cells. Renal progenitor cells transplanted to conditional kidney-deficient fetuses induced the formation of a new nephron in the fetus that is connected to the host ureteric bud.

Conclusions

We developed a cell transplantation method for midstage to late-stage fetuses. In vivo kidney regeneration from renal progenitor cells using the renal developmental environment of the fetus shows promise. Our findings suggest that fetal transplantation methods may contribute to organ regeneration and developmental research.
Key moments of morphological examination of pancreatic tumors considering modern morphological classification
Authors: Paklina O. V. , Setdikova G. R. , Rotin D. L.
Published: September 2019

Modern histological and molecular classification of endocrine and exocrine pancreatic tumorsis reviewed. Step-bystep algorithm of pancreatic complex dissection and detailed histological protocols are described.
Open, laparoscopic and robot-assisted surgery for periampullary cancer (outcomes, advantages, drawbacks)
Authors: Khatkov I. E. , Tsvirkun V. V. , Izrailov R. E. , Mikhnevich M. V. , Vasnev O. S. , Efanov M. G. , Tyutyunnik P. S. , Baychorov M. E. , Andrianov A. V. , Elizarova N. I. , Kazakov I. V. , Vankovich A. N.
Published: September 2019

Objective. To assess the role of minimally invasive surgery for periampullary cancer.

Material and methods. A systematic literature review of original articles devoted to comparison of open, laparoscopic and robot-assisted pancreatoduodenectomy was performed. Also, our own experience of minimally invasive pancreatoduodenectomy is shown.

Results. Articles included in the analysis showed comparable results of minimally invasive and open pancreatoduodenectomy regarding morbidity, mortality and oncological outcomes. Laparoscopic and robot-assisted pancreatoduodenectomy are associated with significantly less intraoperative blood loss and length of postoperative hospital-stay. However, these procedures require more time.

Conclusion. Minimally invasive surgery does not worsen perioperative outcomes and may be considered as an alternative to open procedures in selective patients. Minimally invasive techniques should be implemented in highvolume pancreatic centers only with specialists experienced in minimally invasive surgery.
Minimally invasive technologies for ablation of pancreatic malignancies
Authors: Vetshev P. S. , Chzhao A. V. , Ionkin D. A. , Stepanova Yu. A. , Zhavoronkova O. I. , Kulezneva Yu. V. , Melekhina O. V. , Panchenkov D. N. , Astakhov D. A. , Ivanov Yu. V. , Bruslik S. V. , Sviridova T. I.
Published: September 2019

Minimally invasive ablative technologies is a promising direction in the treatment of some cancer patients including pancreatic cancer. Cryodestruction, radiofrequency ablation, irreversible electroporation and ultrasound ablation show encouraging results regarding destruction of tumor tissue, cytoreduction. These methods are associated with small number of complications and relatively easy to tolerate by patients. However, there is no single approach to their use in the complex treatment of these patients. Accumulation of data followed by comparative analysis of various ablation techniques is being carried out in many specialized clinics of the world including national hospitals. Ablation mechanisms of technologies, literature data and the authors' own experience in the treatment of pancreatic cancer are reported in the article. Further randomized prospective trials are required to determine the role of ablation methods in the complex treatment of tumors of parenchymal organs including pancreatic cancer.
Operability boundaries in pancreatic cancer: what is the limit?
Authors: Subbotin V. V , Dushin I. N. , Zabaluev D. A. , Kamnev S. A. , Klimov A. A. , Malakhova A. A. , Osokin Ya. A.
Published: September 2019

Preoperative diagnosis of comorbidities is essential for identifying the limiting factors inperioperative period and correct assessment of patient’s ability to undergo surgery. Severe concomitant diseases followed by advanced risk of adverse events require development and implementation of the strategy of preoperative preparation of patient. These measuress hould include both medicamentous and surgical correction of concomitant diseases in order to reduce perioperative risks. Thus, multidisciplinary perioperative approach makes it possible to reduce significantly the risks and mortality in patients undergoing surgery for pancreatic cancer. Diagnosis and correction of limiting factors approach operability to absolute one. However, operation should be avoided or alternative approaches should be preferred if co-morbidities are significant and failed to be corrected.
Políticas e práticas territoriais socialmente necessárias
Authors: Neto Manoel Lemes da Silva , Oliveira Fabiano Melo Gonçalves de , Carandina Thiago
Published: September 2019

O objetivo é discutir a insuficiência das condições por meio das quais os instrumentos da legislação urbanística são formulados e postos em prática. Parte-se do pressuposto de que há condições históricas que podem contribuir, e até mesmo produzir, arcabouços normativos com horizontes democráticos concretos, amplos, e sob perspectiva redistributiva. A existência de pré-condições históricas para a constituição do Período Popular da História, propõe planos analíticos inovadores, a exemplo da noção elementar do “socialmente necessário”. Esses elementos fundam-se na compreensão do “território usado” como instância ativa da sociedade e categoria analítica essencial para se pensar o futuro. Examina-se os limites históricos que conduzem análises, projetos e planos que orbitam em torno da noção seminal do “direito à cidade”, e especula-se sobre a urgência de se pensar os lugares como dimensão político-institucional imprescindível para alçar o direito ao patamar do socialmente necessário. Para a apreensão de possibilidades concretas, de natureza insurgente e que se apresentam no presente histórico, especula-se sobre inter-relações indissociáveis e socialmente necessárias dos agentes sociais no processo de tomada de decisões das políticas públicas de caráter territorial.

PALAVRAS-CHAVE: Política urbana. Atores sociais. Ação política. Território usado. Período popular da história.
Fachada Verde e Conforto Térmico em Escritório Universitário
Authors: Silveira Guilherme William Petrini da , Padovan Leonardo Diba Gonçalves , Fontes Maria Solange Gurgel de Castro
Published: September 2019

O sombreamento de fachadas e aberturas de um edifício constitui-se uma das principais estratégias bioclimáticas passivas para melhoria do conforto e desempenho térmico de seus ambientes. Neste sentido o uso de fachadas verdes em superfícies verticais da edificação funciona como dispositivo de controle solar dinâmico, uma vez que proporciona diferentes taxas de transmissão solar, variando conforme clima e estação. Aprimorando o debate sobre os benefícios desta estratégia para arquitetura, o presente trabalho tem como objetivo a avaliação do grau de conforto térmico proporcionado pelo uso de fachada verde com a espécie Wisteria Floribunda (Glicínia) em dois ambientes de escritório do bloco administrativo/professores da Faculdade Integradas de Ourinhos, localizada em Ourinhos-SP, Zona Bioclimática 3. A metodologia utilizada baseou-se em simulações computacionais com o software Energyplus v. 8.9.0, comparando-se os resultados das temperaturas operativas provenientes dos cálculos do programa com a zona de conforto definidas pelo modelo de conforto adaptativo da norma americana ASHRAE 55 (2013). Na análise dos resultados observou-se que introdução da estratégia passiva de sombreamento na fachada oeste proporcionou aumento do conforto em até 9% durante período de uso da edificação. Na comparação do grau hora de desconforto para frio e calor constatou-se melhoria de até 39% no desempenho térmico das salas analisadas.

PALAVRAS-CHAVE: Fachada verde; Conforto térmico; Desempenho térmico. Escritório.
Metodologia de Análise Urbana Tipo-Morfológica: Transformações Urbanas no Bairro Aparecida em Uberlândia-MG
Authors: Cocozza Glauco de Paula , Bertuluci Gabriela de Oliveira , Silva Bárbara Oliveira , Brandão Schirley Crisitane de Oliveira
Published: September 2019

O presente artigo consiste em uma análise de uma região do bairro Aparecida em Uberlândia-MG com o objetivo de identificar as implicações sociais e materiais da forma urbana, o que demandou uma “arqueologia” do tecido urbano para compreender quais aspectos podem ser responsáveis por seus efeitos. A análise foi feita a partir de duas abordagens distintas, mas, complementares: histórico-geográfica de M. R. G. Conzen (1960); e tipológico-processual de Saverio Muratori (1959, 1963). Para tanto, foi feita uma breve contextualização dos métodos e procedimentos analíticos utilizados por Conzen e Muratori e dos procedimentos que foram empregados de fato na análise do bairro. Assim, seguindo os métodos e procedimentos analíticos de Conzen, para identificar e definir os períodos e regiões morfológicas do tecido urbano, foi realizada a divisão tripartite de análise da paisagem: planta, volume edificado e uso do solo. Quanto ao procedimento analítico desenvolvido por Muratori, foi feita a análise de dados qualitativos com a identificação das constantes tipológicas no tecido urbano. Por fim, os resultados alcançados levam a uma definição mais clara do bairro como parte do tecido urbano em que se insere e da lógica espacial existente.

PALAVRAS-CHAVE: Forma Urbana; Transformações Urbanas; Tipo-morfologia
Protocol for detect Trypanosoma cruzi by indirect methods as IIF, ELISA and IHA from serological samples v1
Authors: Méndez Claudia , Clara Duque Maria , Romero Yanira , Pérez Julie , Rodríguez Omaira , A. Correa-Cárdenas Camilo , Teresa Alvarado Maria , Cantillo-Barraza Omar
Published: September 2019

This is the protocolto be used in the diagnosis of Chagas disease in Colombia, according to the algorithm suggested by Health and Social Protection Ministery with the National Health Institute.
UC Davis - Tissue TG & TC Protocol v2
Authors: Havel Peter
Published: September 2019

Summary: The lipid extraction method is based on work by Folch 1957. Tissue is homogenized and put into a 2:1 chloroform and methanol mix. The mix is separated into two phases by adding a salt solution. The upper phase contains any non-lipid substances and the lower phase contains the chloroform with the lipids. The upper phase is removed and a sample is taken from the bottom phase and evaporated. The sample is reconstituted with 2-propanol with a volume to bring it to the desired concentration to assay. Triglycerides are enzymatically hydrolyzed by lipase to free fatty acids and glycerol. The glycerol is phosphorylated by adenosine triphosphate (ATP) with glycerol kinase (GK) to produce glycerol-3- phosphate and adenosine diphosphate. Glycerol-3-phosphate is oxidized by dihydroxyacetone phosphate (DAP) by glycerolphosphate oxidase producing hydrogen peroxide (H²O²). In a Trinder5 type color reaction catalyzed by peroxidase, the H²O² reacts with 4-aminoantipyrine (4-AAP) and 3,5-dichloro-2- hydroxybenzene sulfonate (DHBS) to produce a red colored dye. The absorbance of this dye is proportional to the concentration of triglycerides present in the sample. Cholesterol esters are enzymatically hydrolysed by cholesterol esterase to cholesterol and free fatty acids. Free cholesterol, including that originally present, is then oxidized by cholesterol oxidase to cholest-4-en-3- one and hydrogen peroxide. The hydrogen peroxide combines with HBA and 4-aminoantipyrine to forma chromophore (quinoneimine dye) which may be quantitated at 500-550nm.
UC Davis - Metabolomics: Primary metabolites by GC-TOF MS v2
Authors: Fiehn Oliver
Published: September 2019

Summary: Description of standard setting for analysis of derivatized samples ready for injection into a Leco Pegasus III or Pegasus IV gas chromatograph-time of flight mass spectrometer. References: Fiehn O, Wohlgemuth G, Scholz M, Kind T, Lee DY, Lu Y, Moon S, Nikolau BJ (2008) Quality control for plant metabolomics: Reporting MSI-compliant studies. Plant J. 53, 691-704
Spatial metabolomics of in situ, host-microbe interactions (practical guide for combining MALDI-MSI and FISH microscopy on the same section) v1
Authors: Geier Benedikt , Sogin Emilia , Michellod Dolma , Janda Moritz , Kompauer Mario , Spengler Bernhard , Dubilier Nicole , Liebeke Manuel
Published: September 2019

Spatial metabolomics describes the location and chemistry of small molecules involved in metabolic phenotypes, defense molecules and chemical interactions in natural communities. Most current techniques are unable to spatially link the genotype and metabolic phenotype of microorganisms in situ at a scale relevant to microbial interactions. Here, we present a spatial metabolomics pipeline (metaFISH) that combines fluorescence in situ hybridization (FISH) microscopy and high-resolution atmospheric pressure mass spectrometry imaging (AP-MALDI-MSI) to image host-microbe symbioses and their metabolic interactions. metaFISH aligns and integrates metabolite and fluorescent images at the micrometer-scale for a spatial assignment of host and symbiont metabolites on the same tissue section. To illustrate the advantages of metaFISH, we mapped the spatial metabolome of a deep-sea mussel and its intracellular symbiotic bacteria at the scale of individual epithelial host cells. Our analytical pipeline revealed metabolic adaptations of the epithelial cells to the intracellular symbionts, a variation in metabolic phenotypes in one symbiont type, and novel symbiosis metabolites. metaFISH provides a culture-independent approach to link metabolic phenotypes to community members in situ – a powerful tool for microbiologists across fields.
U Michigan - Illumina 16S rRNA gene sequencing using DNA v2
Authors: Young Vincent
Published: September 2019

Summary: This protocol is for the submission of DNA to generate libraries for 16S rRNA sequencing, which can be used for bacterial community analysis and detect variations in the microbiota under differing conditions. DNA is aliquoted into 96 well plates, which are properly packaged and shipped to the MMPC and libraries are prepared for community analysis as previously described by Seekatz et al. (1). Briefly, barcoded dual-index primers specific to the V4 region of the 16S rRNA gene amplify the DNA (2). PCR reactions are composed of 5 μL of 4 μM equimolar primer set, 0.15 μL of AccuPrime Taq DNA High Fidelity Polymerase, 2 μL of 10x AccuPrime PCR Buffer II (Thermo Fisher Scientific, catalog no. 12346094), 11.85 μL of PCR-grade water, and 1 μL of DNA template. The PCR conditions used consisted of 2 min at 95°C, followed by 30 cycles of 95°C for 20 s, 55°C for 15 s, and 72°C for 5 min, followed by 72°C for 10 min. Each PCR reaction is normalized using the SequalPrep Normalization Plate Kit (Thermo Fisher Scientific, catalog no. A1051001). The normalized reactions are pooled and quantified using the Kapa Biosystems Library qPCR MasterMix (ROX Low) Quantification kit for Illumina platforms (catalog no. KK4873). The Agilent Bioanalyzer is used to confirm the size of the amplicon library (~399 bp) using a high-sensitive DNA analysis kit (catalog no. 5067-4626). Pooled amplicon library is then sequenced on the Illumina MiSeq platform using the 500 cycle MiSeq V2 Reagent kit (catalog no. MS-102-2003) according to the manufacturer's instructions with modifications of the primer set with custom read 1/read 2 and index primers added to the reagent cartridge. The“Preparing Libraries for Sequencing on the MiSeq” (part 15039740, Rev. D) protocol was used to prepare libraries with a final load concentration of 5.5 pM, spiked with 15% PhiX to create diversity within the run. FASTQ files are distributed to the client when the 2 x 250 bp sequencing completes. References: 1. Seekatz AM, Theriot CM, Molloy CT, Wozniak KL, Bergin IL, Young VB. 2015. Fecal Microbiota Transplantation Eliminates Clostridium difficile in a Murine Model of Relapsing Disease. Infect Immun 83:3838-3846. 10.1128/IAI.00459-15. 2. Kozich JJ, Westcott SL, Baxter NT, Highlander SK, Schloss PD. 2013. Development of a dual- index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform. Appl Environ Microbiol 79:5112–5120. 10.1128/AEM.01043-13.
Organization for Lab Data using Bash v1
Authors: Comrie Courtney
Published: September 2019

Organization is important in the IPA and MSBIL
Effect of Limb Salvage by Excimer Laser Debulking plus Low-pressure Balloon Inflation Without Stent Deployment in Patients with Infrapopliteal Vessel Disease v1
Authors: Yang Yuanpo , Lin Tsung-Hsien , Chou Chen-Ying , Lee Cheng-Liang , Huang Hung-Kai , Wu Xian-Nin , Chen Ching-Pei
Published: September 2019

BACKGROUND: Patients with critical limb ischemia (CLI) often exhibit long, diffuse, totally occluded, and heavily calcified infrapopliteal (IP) lesions. Peripheral excimer laser atherectomy (PELA) has been proposed to increase the treatment success for complex IP lesions. This study evaluated the limb salvage after PELA plus low-pressure balloon inflation (LPBI) without stent deployment in CLI patients with severe IP disease. METHODS: We retrospectively evaluated for 82 consecutive patients with 125 IP vessels who underwent PELA plus LPBI in a 4-year period. Technical success was defined as IP straight-line flow being achieved below the malleolus. The binary logistic regression was performed to find factors associated with 6-month limb salvage RESULTS: A total of 113 vessels (90.4%) were totally occluded, and none of the patients received a stent. Twenty-one percentage of the patients were octogenarians, and 84% of the patients were Rutherford-Becker class 5 and 6. The technical success rate was 72% and 6-month limb salvage rate was 77%. Binary logistic regression showed age, smoking history and technical success were independent predictors for 6-month limb salvage. CONCLUSIONS: PELA plus LPBI without stent deployment is a reasonable option for treating complex IP lesions in patients with CLI. Technical success plays an important role in 6-month limb salvage
Genomic DNA extraction from diatom P. multistriata v3
Authors: Manfellotto Francesco , Teresa Russo Monia , Annunziata Rossella , Ferrante Mariella
Published: September 2019

Genomic DNA extraction from diatom P. multistriata
Ligation Protocol with T4 DNA Ligase v1
Authors: Balletbó Alba
Published: September 2019

The final step in the construction of a recombinant plasmid is connecting the insert DNA (gene or fragment of interest) into a compatibly digested vector backbone. This is accomplished by covalently connecting the sugar backbone of the two DNA fragments. This reaction, called ligation, is performed by the T4 DNA ligase enzyme. The DNA ligase catalyzes the formation of covalent phosphodiester linkages, which permanently join the nucleotides together. After ligation, the insert DNA is physically attached to the backbone and the complete plasmid can be transformed into bacterial cells for propagation. The majority of ligation reactions involve DNA fragments that have been generated by restriction enzyme ligation (See Protocols). Most restriction enzymes digest DNA asymmetrically across their recognition sequence, which results in a single stranded overhang on the digested end of the DNA fragment. The overhangs, called "sticky ends", are what allow the vector and insert to bind to each other. When the sticky ends are compatible, meaning that the overhanging base pairs on the vector and insert are complementary, the two pieces of DNA connect and ultimately are fused by the ligation reaction.
Colony PCR (Protocol for Thermo Scientific™ Phire™ Hot Start II DNA Polymerase) v1
Authors: Balletbó Alba
Published: September 2019

Thermo Scientific™ Phire™ Hot Start II DNA Polymerase is a novel DNA polymerase designed for use in all routine and high throughput PCR applications. A special DNA binding domain linked to the Phire Hot Start II DNA Polymerase enhances the processivity of the polymerase, enabling short extension times and improved yields. The polymerase is also capable of amplifying long DNA fragments, such as the 7.5 kb genomic DNA used in Thermo Scientific quality control assays. Phire Hot Start II DNA Polymerase provides 2-fold fidelity compared to Taq DNA polymerase. The hot start modification of the polymerase is based on the Affibody™ inactivation method1,2 . It inhibits DNA polymerase activity at ambient temperatures, thus preventing amplification of non-specific products. At polymerization temperatures the Affibody molecule is released, rendering the polymerase fully active. Phire Hot Start II DNA Polymerase generates blunt ends in the amplification products. It does not possess the 5’→3’ exonuclease activity needed for hydrolysis experiments.
Method of measuring simulated weight-bearing in vivo forces acting on medial tibial plafond v1
Authors: E Graham Michael
Published: September 2019

This protocol describes a method to simulate and measure weight-bearing in vivo forces acting on the medial knee joint. Furthermore, the effects of subtalar joint instability will be studied to determine if a connection to altered knee forces exists. The method to discover if there is a connection between subtalar joint instability and increased forces acting on the medial knee joint will be evaluated by measuring the knee joint forces in an above knee cadaveric specimens that have subtalar joint instability, diagnoses with open and closed chain range of motion testing, confirmed by simulate weightbearing x-rays (dorsoplantar and lateral). The insertion of a sinus tarsi stent will normalize the subtalar joint range of motion and this will also be shown via the range of motion testing and radiographic examination showing realignment of the talotarsal joint. The simulation of weight-bearing forces will be measured with and without the insertion of the sinus tarsi stent.
Phage stock preparation v1
Authors: Ceelen Marijn
Published: September 2019

A protocol for phage lambda and phage T7 phage stock preparation
pAmpPSBA Plasmid Map v1
Authors: Nimmo Isabel , Nisbet Ellen , Barbrook Adrian , Howe Christopher
Published: September 2019

A plasmid map for the Amphidinium carterae pAmpPSBA construct
U Michigan - DNA Extraction for Illumina 16S rRNA Extraction v2
Authors: Young Vincent
Published: September 2019

Summary: This protocol is for the submission of tissue/fecal samples for DNA extraction and subsequent processing to generate libraries for 16S rRNA sequencing, which can be used for bacterial community analysis and detect variations in the microbiota under differing conditions. Up to 250 uL or 0.25 g of sample is added to each well of the Bead plate provided by the MMPC. Plates are properly packaged and shipped to the MMPC and processed using the Qiagen MagAttract PowerMicrobiome kit DNA/RNA kit (Qiagen, catalog no. 27500-4-EP) on the EpMotion 5075 (Eppendorf) liquid handler. DNA is lysed using mechanical bead beating and extracted using magnetic bead technology. Extracted DNA is then used to generate 16S rRNA libraries for community analysis. The process used for library generation has been previously described by Seekatz et al. (1). Briefly, barcoded dual-index primers specific to the V4 region of the 16S rRNA gene amplify the DNA (2). PCR reactions are composed of 5 µL of 4 µM equimolar primer set, 0.15 µL of AccuPrime Taq DNA High Fidelity Polymerase, 2 µL of 10x AccuPrime PCR Buffer II (Thermo Fisher Scientific, catalog no. 12346094), 11.85 µL of PCR-grade water, and 1 µL of DNA template. The PCR conditions used consisted of 2 min at 95°C, followed by 30 cycles of 95°C for 20 s, 55°C for 15 s, and 72°C for 5 min, followed by 72°C for 10 min. Each PCR reaction is normalized using the SequalPrep Normalization Plate Kit (Thermo Fisher Scientific, catalog no. A1051001). The normalized reactions are pooled and quantified using the Kapa Biosystems Library qPCR MasterMix (ROX Low) Quantification kit for Illumina platforms (catalog no. KK4873). The Agilent Bioanalyzer is used to confirm the size of the amplicon library (~399 bp) using a high-sensitive DNA analysis kit (catalog no. 5067-4626). Pooled amplicon library is then sequenced on the Illumina MiSeq platform using the 500 cycle MiSeq V2 Reagent kit (catalog no. MS-102-2003) according to the manufacturer's instructions with modifications of the primer set with custom read 1/read 2 and index primers added to the reagent cartridge. The “Preparing Libraries for Sequencing on the MiSeq” (part 15039740, Rev. D) protocol was used to prepare libraries with a final load concentration of 5.5 pM, spiked with 15% PhiX to create diversity within the run. FASTQ files are distributed to the client when the 2 x 250 bp sequencing completes. References: 1. Seekatz AM, Theriot CM, Molloy CT, Wozniak KL, Bergin IL, Young VB. 2015. Fecal Microbiota Transplantation Eliminates Clostridium difficile in a Murine Model of Relapsing Disease. Infect Immun 83:3838-3846. 10.1128/IAI.00459-15. 2. Kozich JJ, Westcott SL, Baxter NT, Highlander SK, Schloss PD. 2013. Development of a dual- index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform. Appl Environ Microbiol 79:5112–5120. 10.1128/AEM.01043-13.
Polymorphism v1
Authors: Esparza Cecilia
Published: September 2019

Background: Depressive symptoms are diagnosed using symptom scales by a psychiatric doctor but pathophysiology is unclear. Serotonin (5-HT) plays an important role in neural activity and social behavior. 5-HT transporter (5-HTT) is an important regulator of brain serotonin levels and its reuptake. The 5-HTT gene-linked polymorphic region (5-HTTLPR) is associated with depression. Aim: The aim of this study was to further characterize the role and impact of serotonin levels in plasma and the 5HTTPLR polymorphism in the depressive symptoms present in older adults. Methods:We included 84 aging adult participants. Plasma serotonin levels was determined by ELISA method and the 5-HTTLPR genotype was analyzed by polymerase chain reaction (PCR). Results and discussion:33 (39.3%) participants presented with depressive symptoms. The median level of serotonin in blood was 204.34 ± 93.88 ng/mL. There was an association between plasma serotonin levels and depressive symptoms (p= 0.001). The 5-HTTLPR analysis showed that of the 84 older adults, 30 (35.7%) were SS genotype, 9 (10.7%) LL genotype and 45 (53.6%) heterozygous. No association was found between the 5-HTTLPR polymorphism and depressive symptoms; (odds ratio) OR 1.21 95%IC 0.6-2.30 and plasma serotonin levels OR 0.73 95%IC 0.39-1.38. Conclusions:Depressive Symptomsare associated with low serotonin levels in the plasma but not with a 5-HTTLPR polymorphism in a population of aging Mexican adults. There was also a protective effect against depressive symptoms associated with high serotonin levels in the presence of the S allele.
Tissue Freezing for seqFISH v1
Authors: Dar Nina , Cai Long
Published: September 2019

Once a tissue is received, it should be frozen using the following protocol:
Responsabilidade Socioambiental: um estudo em Empresa de Transporte Coletivo de Passageiros (ETCP)
Authors: Weis Jeniffer Campos
Published: September 2019

Este estudo teve como objetivo descrever como a ETCP está desenvolvendo a sua responsabilidade socioambiental. A pesquisa é de natureza aplicada; com abordagem qualitativa; descritiva quanto ao objetivo e quanto ao procedimento técnico utilizado se enquadra como pesquisa de campo. Os dados foram coletados por meio de um instrumento de pesquisa (questionário) respondido por diretores e gerentes, contendo 14 (catorze) perguntas abertas. Nos depoimentos colhidos, foi possível perceber que na empresa são desenvolvidas diversas ações voltadas ao meio ambiente, como a renovação da frota para reduzir a emissão de gás carbônico; estação de tratamento de água, de pintura e separação de resíduos, ao mesmo tempo em que são desenvolvidas ações que objetivam beneficiar os colaboradores e familiares. Os respondentes também mencionaram a repercussão positiva com os colaboradores decorrente de premiações recebidas pela empresa. Outro elemento que merece destaque é o fato de os diretores e os gerentes estarem comprometidos com a elaboração do balanço social da empresa, em que são divulgadas as ações socioambientais desenvolvidas.
Reflexos da nova sistemática de cálculo do Simples Nacional na carga tributária: uma análise das alterações promovidas pela LC n.º 155/2016 e Lei Estadual n.º 11.031/2017 para as empresas comerciais no Estado da Paraíba
Authors: Aires Matheus De Farias , Menezes José Elinilton Cruz de , Dieng Mamadou , Toledo Sidney Soares de
Published: September 2019

A presente pesquisa teve como objetivo geral analisar o impacto tributário decorrente da Lei Complementar n.º 155/2016 e da Lei Estadual n.º 11.031/2017 para as empresas do comércio paraibano enquadradas no Simples Nacional (SN). Trata-se de uma pesquisa de caráter exploratório, descritiva, bibliográfica e com uma abordagem quantitativa. A fim de observar os reflexos gerados pela alteração do cálculo do SN nas empresas do comercio paraibano, foi escalonado um Faturamento Mensal Médio (FMM) a cada dez mil reais. Evidenciou-se que das trinta faixas de FMM analisadas apenas em 7 houve reduções da carga tributária do SN, quando comparado à nova sistemática de cálculo com a antiga. Assim sendo, as demais 23 faixas expressaram acréscimo da carga tributária no novo cálculo. Em relação às reduções, percebe-se que variam de 0,02 a 0,64 pontos percentuais, enquanto ao observar os acréscimos, percebe-se que estes variam de 0,04 a 1,31 pontos percentuais. Com isso, pôde-se concluir que a nova sistemática de cálculo tem como principais reflexos na carga tributária o aperfeiçoamento da obediência ao princípio da progressividade, assim como o aumento do montante monetário dos tributos envolvidos no Simples Nacional.
Materialidade aplicada a relatórios de sustentabilidade assegurados: uma abordagem crítica à luz da pesquisa contábil
Authors: Da Cunha Estephanye Paganotti , Fiorin Ivone , Faller Renato Loureiro
Published: September 2019

Este ensaio teórico aborda lacunas quanto à pesquisa contábil ambiental e as limitações apresentadas por relatórios socioambientais. Indagam-se alguns pontos ainda não respondidos sobre sustentabilidade, como o fato de o assunto ser pouco pesquisado no Brasil. Isso posto, observa-se em foco o que é chamado na literatura de Ciência da Sustentabilidade, uma linha de conhecimento multidisciplinar que envolve diversas áreas de conhecimento, permitindo a experimentação e testes, em vez de apenas fazer uma seleção de ciência específica para encontrar a solução mais acertada. A materialidade contábil no âmbito da pesquisa ambiental, tendo em vista as óticas dos organismos mundiais, tais como, Financial Accounting Standards Board (Ffasb), Securities And Exchange Commission (Sec), International Accounting Standards Board (Iasb), Public Company Accounting Oversight Board (PCAOB), que, apesar de ter como ponto positivo ser flexível, apresenta, ao mesmo tempo, como ponto negativo, essa mesma flexibilidade, que permite aos relatórios ambientais maior subjetividade e baixa objetividade. A referida flexibilidade pode fazer com que a empresa demonstre o que foi considerado bom, omitindo o que a instituição considere ruim na sua visão. Sendo assim, questionamentos são levantados sobre o escopo da Ciência da Sustentabilidade, da efetividade dos relatórios socioambientais apresentados e da clareza das demonstrações socioambientais, mediante a presença, ou não, de dispositivos legais.
Análise dos relatórios de auditoria independente das companhias brasileiras listadas no Novo Mercado da B3 S.A
Authors: Da Silva Márcia Fontoura , Venturini Lauren Dal Bem , Andre Fernando Cafruni , Bianchi Márcia
Published: September 2019

O estudo analisa as informações divulgadas pelos auditores independentes no Novo Relatório de Auditoria das empresas do segmento Novo Mercado da B3 S.A. Utilizou-se a pesquisa qualitativa, quantitativa, descritiva e documental, analisando as informações dos relatórios de auditoria independente, de 138 empresas em 2016 e 145 em 2017, no tocante aos Principais Assuntos de Auditoria (PAAs) e às respostas destes, tipo de opinião, às ênfases e os outros assuntos de auditoria. Identificaram-se 396 PAAs, em 2016, e 399, em 2017, cada qual com seus procedimentos de auditoria, sendo Valor Recuperável de Ativos Não Financeiros, Receita e Contingências, nessa ordem, como os mais reportados nos dois anos avaliados. Apenas cinco relatórios de auditoria apresentaram opinião modificada em 2016 e sete em 2017 e, quanto às ênfases, em 2016 e 2017, sobressaíram-se aspectos relacionados à reapresentação e à aplicação adicional de norma de atividade específica na elaboração das demonstrações contábeis, e o Demonstrativo de Valor Adicionado foi o mais destacado, em ambos os anos, no parágrafo de outros assuntos de auditoria. O estudo auxilia, especialmente, investidores e analistas financeiros averiguarem os destaques elencados pelos auditores independentes, nas empresas categorizadas no nível de Governança Corporativa Novo Mercado da B3.
Características das certificações profissionais (obrigatórias e voluntárias) das áreas Contábil e de Finanças
Authors: Borba José Alonso , Soares José Guilherme , Minatti Ferreira Denize Demarche , Maragno Lucas Martins Dias
Published: September 2019

O artigo apresenta as características das principais certificações obrigatórias e não obrigatórias com maior relevância internacional para profissionais das áreas Contábil e de Finanças. Os órgãos certificadores possuem, no mínimo, dez anos de existência e mais de 50 mil membros. Além disso, são líderes em seus campos de atuação. Destaca-se que a obrigatoriedade, tanto no Brasil como nos Estados Unidos surgiu devido à importância da Contabilidade,  em que órgãos reguladores optaram pela habilitação mediante certificações obrigatórias, enfrentando um aumento expressivo na demanda imediatamente após casos de irregularidades nas empresas. As certificações não obrigatórias, criadas normalmente por associações ou organizações de profissionais de mercado, vêm aumentando em termos de quantidade. Dentre os requisitos para obtenção das certificações, destaca-se a necessidade de diploma de graduação ou experiência profissional mínima equivalente. Os benefícios apontados recaem no aumento em compensações salariais ao longo da carreira, com diferença expressiva quando comparados aos pares sem certificação.