bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical Correlation of Transcription Factor SOX3 in Cancer: Unveiling Its Role in Tumorigenesis
Authors: Del Puerto Helen Lima , Miranda Ana Paula G. S. , Qutob Dinah , Ferreira Enio , Silva Felipe H. S. , Lima Bruna M. , Carvalho Barbara A. , Roque-Souza Bruna , Gutseit Eduardo , Castro Diego C. , Pozzolini Emanuele T. , Duarte Nayara O. , Lopes Thacyana B. G. , Taborda Daiana Y. O. , Quirino Stella M. , Elgerbi Ahmed , Choy John S. , Underwood Adam
Published: June 2024

Members of the SOX (SRY-related HMG box) family of transcription factors are crucial for embryonic development and cell fate determination. This review investigates the role of SOX3 in cancer, as aberrations in SOX3 expression have been implicated in several cancers, including osteosarcoma, breast, esophageal, endometrial, ovarian, gastric, hepatocellular carcinomas, glioblastoma, and leukemia. These dysregulations modulate key cancer outcomes such as apoptosis, epithelial-mesenchymal transition (EMT), invasion, migration, cell cycle, and proliferation, contributing to cancer development. SOX3 exhibits varied expression patterns correlated with clinicopathological parameters in diverse tumor types. This review aims to elucidate the nuanced role of SOX3 in tumorigenesis, correlating its expression with clinical and pathological characteristics in cancer patients and cellular modelsBy providing a comprehensive exploration of SOX3 involvement in cancer, this review underscores the multifaceted role of SOX3 across distinct tumor types. The complexity uncovered in SOX3 function emphasizes the need for further research to unravel its full potential in cancer therapeutics.
Mapping Strategies for Strengthening Safety Culture: A Scoping Review
Authors: Pacenko Cristiane de Lima , Figueiredo Karla Crozeta , Nunes Elisabete , Cruchinho Paulo , Lucas Pedro
Published: June 2024

Background: Twenty years after the “To Err Is Human” report, one in ten patients still suffer harm in hospitals in high-income countries, highlighting the need to strengthen the culture of safety in healthcare. This scoping review aims to map patient safety culture strengthening strategies described in the literature. Method: This scoping review follows the JBI methodology. It adhered to all scoping review checklist items (PRISMA-ScR) with searches in the Lilacs, MedLine, IBECS, and PubMed databases and on the official websites of Brazilian and North American patient safety organizations. The research took place during the year 2023. Results: In total, 58 studies comprising 52 articles and 6 documents from health organizations were included. Various strategies were identified and grouped into seven categories based on similarity, highlighting the need for a comprehensive organizational approach to improve patient care. The most described strategies were communication (69%), followed by teamwork (58.6%) and active leadership (56.9%). Conclusion: The identified strategies can promote the development of a culture in which an organization can achieve patient safety, involving practices and attitudes that reduce risks and errors in healthcare. However, the identification of strategies is limited because it is restricted to certain databases and websites of international organizations and does not cover a broader spectrum of sources. Furthermore, the effectiveness of these strategies in improving patient safety culture has not yet been evaluated.
Midwifery Students’ Views on NANDA-I Diagnoses and Care Plans Used in Clinical Practice
Authors: Baran Leyla , Yeşil Yeşim
Published: June 2024

Background: Developing a care plan for patients is not specific to nursing or midwifery; it is the case in every situation where patients seek help from care providers. Midwifery students draw upon NANDA-I diagnoses while developing care plans (CPs) in clinical practices, and plan and apply their care accordingly. This study aims to identify the views of midwifery students on the NANDA-I diagnoses and CPs that they use in clinical practice. Methods: This descriptive and cross-sectional study was conducted with 222 students between September and December 2022. The research data were collected through face-to-face interviews using a questionnaire based on NANDA-I diagnoses and CPs. In line with the existing literature, the questionnaire was designed by two academicians who specialized in nursing fundamentals and midwifery. The questionnaire consisted of closed-ended (8 questions) and open-ended (7 questions) questions. Data analysis was performed with descriptive statistics and Pearson’s chi-square test. Results: Of 222 students, 57.7% stated that they did not know the definition of a CP. It was found that 46.8% of the students felt inadequate at developing CPs. Furthermore, the students stated that they found the CP training provided during their undergraduate study partially sufficient, with a rate of 52.7%, and 16.7% of midwifery students offered suggestions about CP teaching. The number of midwifery-related diagnoses of which the students were aware was 19. Conclusions: The study was beneficial in terms of identifying the deficiencies of the students related to NANDA-I diagnoses and developing CPs that might be overcome through education and observing the midwifery-related diagnoses that they used. It is of critical importance to educate midwifery students about midwifery-related NANDA-I diagnoses in order that they embrace midwifery diagnoses and CP learning.
Gene-Based Developments in Improving Quality of Tomato: Focus on Firmness, Shelf Life, and Pre- and Post-Harvest Stress Adaptations
Authors: Nie Hongmei , Yang Xiu , Zheng Shaowen , Hou Leiping
Published: June 2024

Tomato (Solanum lycopersicum) is a widely consumed vegetable crop with significant economic and nutritional importance. This review paper discusses the recent advancements in gene-based approaches to enhance the quality of tomatoes, particularly focusing on firmness, shelf life, and adaptations to pre- and post-harvest stresses. Utilizing genetic engineering techniques, such as Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins 9 (CRISPR/Cas9) and Transcription Activator-like Effector Nucleases (TALENs), researchers have made remarkable progress in developing tomatoes with improved traits that address key challenges faced during cultivation, storage, and transportation. We further highlighted the potential of genetic modifications in enhancing tomato firmness, thereby reducing post-harvest losses and improving consumer satisfaction. Furthermore, strategies to extend tomato shelf life through genetic interventions are discussed, emphasizing the importance of maintaining quality and freshness for sustainable food supply chains. Furthermore, the review delves into the ways in which gene-based adaptations can bolster tomatoes against environmental stresses, pests, and diseases, thereby enhancing crop resilience and ensuring stable yields. Emphasizing these crucial facets, this review highlights the essential contribution of genetic advancements in transforming tomato production, elevating quality standards, and promoting the sustainability of tomato cultivation practices.
Climate Change Projections of Potential Evapotranspiration for the North American Monsoon Region
Authors: Shamir Eylon , Mendoza Fierro Lourdes , Mohsenzadeh Karimi Sahar , Pelak Norman , Tarouilly Emilie , Chang Hsin-I , Castro Christopher L.
Published: June 2024

We assessed and quantified future projected changes in terrestrial evaporative demand by calculating Potential Evapotranspiration (PET) for the North American Monsoon region in the Southwestern U.S. and Mexico. The PET projections were calculated using the daily Penman–Monteith equation. The terrestrial meteorological variables needed for the equation (i.e., minimum and maximum daily temperature, specific humidity, wind speed, incoming shortwave radiation, and pressure) were obtained from the North American–CORDEX initiative. We used dynamically downscaled projections of three CMIP5 GCMs for RCP8.5 emission scenarios (i.e., HadGEM2-ES, MPI-ESM-LR, and GFDL-ESM2M), and each was dynamically downscaled to ~25 km by two RCMs (i.e., WRF and regCM4). All terrestrial annual PET projections showed a statistically significant increase when comparing the historical period (1986–2005) to future projections (2020–2039 and 2040–2059). The regional spatial average of the six GCM-RCM combinations projected an increase in the annual PET of about +4% and +8% for 2020–2039 and 2040–2059, respectively. The projected average 20-year annual changes over the study area range for the two projection periods were +1.4%–+8.7% and +3%–+14.2%, respectively. The projected annual PET increase trends are consistent across the entire region and for the six GCM-RCM combinations. Higher annual changes are projected in the northeast part of the region, while smaller changes are projected along the pacific coast. The main drivers for the increase are the projected warming and increase in the vapor pressure deficit. The projected changes in PET, which represent the changes in the atmospheric evaporative demand, are substantial and likely to impact vegetation and the hydrometeorological regime in the area. Quantitative assessments of the projected PET changes provided by this study should be considered in upcoming studies to develop resilience plans and adaptation strategies for mitigating the projected future changes.
Exploring the Influence of Terrain Blockage on Spatiotemporal Variations in Land Surface Temperature from the Perspective of Heat Energy Redistribution
Authors: Gao Hong , Dong Yong , Zhou Liang , Wang Xi
Published: June 2024

Land surface temperature (LST) is a critical indicator of the earth’s surface environment, which has significant implications for research on the ecological environment and climate change. The influence of terrain on LST is complex due to its rugged and varied surface topography. The relationship between traditional terrain features and LST has been comprehensively discussed in the literature; however, terrain blockage has received less attention and could influence LST by hindering the redistribution of heat energy in mountain regions. Here, we investigate the influence of terrain blockage on the spatiotemporal variation in LST in mountain regions. We first propose a terrain feature framework to characterize the effect of terrain blockage from the perspective of heat energy redistribution and then adopt a random forest model to analyze the relationship between terrain blockage features and LST over a whole year. The results show that terrain blockage significantly influences the spatial heterogeneity of LST, which can be effectively simulated based on terrain blockage features, with a mean deviation of less than 0.15 K. Terrain blockage has a more pronounced influence on LST during the four months from June to September. This influence is also more evident during nighttime than daytime. Regarding LST in mountain regions, local terrain blockage features have a greater influence than global terrain blockage features. In spatial terms, the influence of terrain blockage on LST is uniform. Moreover, the diurnal variation in LST can also be effectively simulated based on terrain blockage. The contribution of this study lies in the finding that terrain blockage can influence the spatiotemporal variation in LST through the process of heat energy redistribution. The terrain blockage features proposed in this study may be useful for other studies of the ecological environment in mountain regions.
Updates on C3 Glomerulopathy in Kidney Transplantation: Pathogenesis and Treatment Options
Authors: Bartoli Giulia , Dello Strologo Andrea , Grandaliano Giuseppe , Pesce Francesco
Published: June 2024

C3 glomerulopathy is a rare disease, characterized by an abnormal activation of the complement’s alternative pathway that leads to the accumulation of the C3 component in the kidney. The disease recurs in more than half of kidney transplant recipients, with a significant impact on graft survival. Recurrence of the primary disease represents the second cause of graft loss after organ rejection. In C3 glomerulopathy, there are several risk factors which can promote a recurrence during transplantation, such as delayed graft function, infection and monoclonal gammopathy. All these events can trigger the alternative complement pathway. In this review, we summarize the impact of C3 glomerulopathy on kidney grafts and present the latest treatment options. The most widely used treatments for the disease include corticosteroids and mycophenolate mofetil, which are already used chronically by kidney transplant recipients; thus, additional treatments for C3 glomerulopathy are required. Currently, several studies using anti-complement drugs (i.e., eculizumab, Ravalizumab, avacopan) for C3 glomerulopathy in kidney transplant patients are ongoing with encouraging results.
Gene Expression Reprogramming by Citrate Supplementation Reduces HepG2 Cell Migration and Invasion
Authors: Miglionico Rocchina , Matera Ilenia , Ventola Giovanna Maria , Marchese Giovanna , Abruzzese Vittorio , Monné Magnus , Ostuni Angela , Bisaccia Faustino
Published: June 2024

Citrate, which is obtained from oxaloacetate and acetyl-CoA by citrate synthase in mitochondria, plays a key role in both normal and cancer cell metabolism. In this work, we investigated the effect of 10 mM extracellular citrate supplementation on HepG2 cells. Gene expression reprogramming was evaluated by whole transcriptome analysis using gene set enrichment analysis (GSEA). The transcriptomic data were validated through analyzing changes in the mRNA levels of selected genes by qRT-PCR. Citrate-treated cells exhibited the statistically significant dysregulation of 3551 genes; 851 genes were upregulated and 822 genes were downregulated. GSEA identified 40 pathways affected by differentially expressed mRNAs. The most affected biological processes were related to lipid and RNA metabolism. Several genes of the cytochrome P450 family were upregulated in treated cells compared to controls, including the CYP3A5 gene, a tumor suppressor in hepatocellular carcinoma (HCC) that plays an important protective role in HCC metastasis. The citrate-induced dysregulation of cytochromes could both improve the effectiveness of chemotherapeutics used in combination and reduce the aggressiveness of tumors by diminishing cell migration and invasion.
Methionine Sulfoxide Speciation in Mouse Hippocampus Revealed by Global Proteomics Exhibits Age- and Alzheimer’s Disease-Dependent Changes Targeted to Mitochondrial and Glycolytic Pathways
Authors: Lopes Filipa Blasco Tavares Pereira , Schlatzer Daniela , Li Mengzhen , Yilmaz Serhan , Wang Rihua , Qi Xin , Ayati Marzieh , Koyutürk Mehmet , Chance Mark R.
Published: June 2024

Methionine oxidation to the sulfoxide form (MSox) is a poorly understood post-translational modification of proteins associated with non-specific chemical oxidation from reactive oxygen species (ROS), whose chemistries are linked to various disease pathologies, including neurodegeneration. Emerging evidence shows MSox site occupancy is, in some cases, under enzymatic regulatory control, mediating cellular signaling, including phosphorylation and/or calcium signaling, and raising questions as to the speciation and functional nature of MSox across the proteome. The 5XFAD lineage of the C57BL/6 mouse has well-defined Alzheimer’s and aging states. Using this model, we analyzed age-, sex-, and disease-dependent MSox speciation in the mouse hippocampus. In addition, we explored the chemical stability and statistical variance of oxidized peptide signals to understand the needed power for MSox-based proteome studies. Our results identify mitochondrial and glycolytic pathway targets with increases in MSox with age as well as neuroinflammatory targets accumulating MSox with AD in proteome studies of the mouse hippocampus. Further, this paper establishes a foundation for reproducible and rigorous experimental MSox-omics appropriate for novel target identification in biological discovery and for biomarker analysis in ROS and other oxidation-linked diseases.
Chemokines and Cytokines in Immunotherapy of Melanoma and Other Tumors: From Biomarkers to Therapeutic Targets
Authors: Reschke Robin , Enk Alexander H. , Hassel Jessica C.
Published: June 2024

Chemokines and cytokines represent an emerging field of immunotherapy research. They are responsible for the crosstalk and chemoattraction of immune cells and tumor cells. For instance, CXCL9/10/11 chemoattract effector CD8+ T cells to the tumor microenvironment, making an argument for their promising role as biomarkers for a favorable outcome. The cytokine Interleukin-15 (IL-15) can promote the chemokine expression of CXCR3 ligands but also XCL1, contributing to an important DC-T cell interaction. Recruited cytotoxic T cells can be clonally expanded by IL-2. Delivering or inducing these chemokines and cytokines can result in tumor shrinkage and might synergize with immune checkpoint inhibition. In addition, blocking specific chemokine and cytokine receptors such as CCR2, CCR4 or Il-6R can reduce the recruitment of tumor-associated macrophages (TAMs), myeloid-derived suppressor cells (MDSCs) or regulatory T cells (Tregs). Efforts to target these chemokines and cytokines have the potential to personalize cancer immunotherapy further and address patients that are not yet responsive because of immune cell exclusion. Targeting cytokines such as IL-6 and IL-15 is currently being evaluated in clinical trials in combination with immune checkpoint-blocking antibodies for the treatment of metastatic melanoma. The improved overall survival of melanoma patients might outweigh potential risks such as autoimmunity. However, off-target toxicity needs to be elucidated.
Arginine Vasotocin Directly Regulates Spermatogenesis in Adult Zebrafish (Danio rerio) Testes
Authors: Zanardini Maya , Zhang Weimin , Habibi Hamid R.
Published: June 2024

The neuropeptide vasopressin is known for its regulation of osmotic balance in mammals. Arginine vasotocin (AVT) is a non-mammalian homolog of this neuropeptide that is present in fish. Limited information suggested that vasopressin and its homologs may also influence reproductive function. In the present study, we investigated the direct effect of AVT on spermatogenesis, using zebrafish as a model organism. Results demonstrate that AVT and its receptors (avpr1aa, avpr2aa, avpr1ab, avpr2ab, and avpr2l) are expressed in the zebrafish brain and testes. The direct action of AVT on spermatogenesis was investigated using an ex vivo culture of mature zebrafish testes for 7 days. Using histological, morphometric, and biochemical approaches, we observed direct actions of AVT on zebrafish testicular function. AVT treatment directly increased the number of spermatozoa in an androgen-dependent manner, while reducing mitotic cells and the proliferation activity of type B spermatogonia. The observed stimulatory action of AVT on spermiogenesis was blocked by flutamide, an androgen receptor antagonist. The present results support the novel hypothesis that AVT stimulates short-term androgen-dependent spermiogenesis. However, its prolonged presence may lead to diminished spermatogenesis by reducing the proliferation of spermatogonia B, resulting in a diminished turnover of spermatogonia, spermatids, and spermatozoa. The overall findings offer an insight into the physiological significance of vasopressin and its homologs in vertebrates as a contributing factor in the multifactorial regulation of male reproduction.
Advancements in Biomarkers and Molecular Targets in Hematological Neoplasias
Authors: Gonçalves Ana Cristina , Alves Raquel , Sarmento-Ribeiro Ana Bela
Published: June 2024

Hematological neoplasias are among the most common cancers worldwide, and the number of new cases has been on the rise since 1990, reaching 1 [...]
Compensatory Modulation of Seed Storage Protein Synthesis and Alteration of Starch Accumulation by Selective Editing of 13 kDa Prolamin Genes by CRISPR-Cas9 in Rice
Authors: Pham Hue Anh , Cho Kyoungwon , Tran Anh Duc , Chandra Deepanwita , So Jinpyo , Nguyen Hanh Thi Thuy , Sang Hyunkyu , Lee Jong-Yeol , Han Oksoo
Published: June 2024

Rice prolamins are categorized into three groups by molecular size (10, 13, or 16 kDa), while the 13 kDa prolamins are assigned to four subgroups (Pro13a-I, Pro13a-II, Pro13b-I, and Pro13b-II) based on cysteine residue content. Since lowering prolamin content in rice is essential to minimize indigestion and allergy risks, we generated four knockout lines using CRISPR-Cas9, which selectively reduced the expression of a specific subgroup of the 13 kDa prolamins. These four mutant rice lines also showed the compensatory expression of glutelins and non-targeted prolamins and were accompanied by low grain weight, altered starch content, and atypically-shaped starch granules and protein bodies. Transcriptome analysis identified 746 differentially expressed genes associated with 13 kDa prolamins during development. Correlation analysis revealed negative associations between genes in Pro13a-I and those in Pro13a-II and Pro13b-I/II subgroups. Furthermore, alterations in the transcription levels of 9 ER stress and 17 transcription factor genes were also observed in mutant rice lines with suppressed expression of 13 kDa prolamin. Our results provide profound insight into the functional role of 13 kDa rice prolamins in the regulatory mechanisms underlying rice seed development, suggesting their promising potential application to improve nutritional and immunological value.
Composite Graph Neural Networks for Molecular Property Prediction
Authors: Bongini Pietro , Pancino Niccolò , Bendjeddou Asma , Scarselli Franco , Maggini Marco , Bianchini Monica
Published: June 2024

Graph Neural Networks have proven to be very valuable models for the solution of a wide variety of problems on molecular graphs, as well as in many other research fields involving graph-structured data. Molecules are heterogeneous graphs composed of atoms of different species. Composite graph neural networks process heterogeneous graphs with multiple-state-updating networks, each one dedicated to a particular node type. This approach allows for the extraction of information from s graph more efficiently than standard graph neural networks that distinguish node types through a one-hot encoded type of vector. We carried out extensive experimentation on eight molecular graph datasets and on a large number of both classification and regression tasks. The results we obtained clearly show that composite graph neural networks are far more efficient in this setting than standard graph neural networks.
Exoscopic versus Microscopic Surgery in 5-ALA-Guided Resection of High-Grade Gliomas
Authors: Garufi Giada , Conti Alfredo , Chaurasia Bipin , Cardali Salvatore Massimiliano
Published: June 2024

Background: Glioma surgery has been remarkably enhanced in the past 2 decades, with improved safety and limited but improved life expectations. The fluorescence-guided resection of high-grade gliomas (HGGs) plays a central role in this sense, allowing a greater extent of resection (EOR). The introduction of exoscopic-guided surgery may be considered in implementing fluorescence techniques over traditional microscopes. We present the application and the advantages of exoscopic-guided surgery compared to microscopic surgery in tumor resection guided by 5-ALA fluorescence in patients with HGGs. Methods: Ten consecutive patients underwent surgery for HGG resection. The surgery was performed via an exoscopic-guided procedure (Olympus ORBEYE) and after the oral administration of Gliolan 5 h before the procedure. During surgery, the procedure shifted to using a microscopic (Kinevo 900, Zeiss) view. The intensity of the fluorescence under the two different procedures was subjectively measured in different picture samples during the surgery on a 1 to 5 (from minimum to maximum) scale. The brightness of the surgical field and the detailing of the anatomy were also analyzed comparatively. Results: Among the ten patients, the histopathological diagnosis was an high-grade glioma in all cases. In nine cases, it was possible to achieve gross total resection. There was no perioperative mortality. The median fluorescence intensity, on a scale of 1–5, was 4.5 in the exoscope group and 3.5 in the microscope group (p < 0.01). Conclusions: The exoscopic-guided surgery adds advantages to traditional fluorescence-guided surgery with 5-aminolevulinic acid. Beyond the important advantage of low cost and the possibility to perform collaborative surgeries, it adds a plain and continuous visualization of the tumor and offers advantages in the surgical field of fluorescence-guided glioma surgery compared to the microscopic-guided one.
The Retention Rate and Safety of JAK Inhibitors in Rheumatoid Arthritis: Real Word Data from a Monocentric Cohort
Authors: Donzella Denise , Bellis Elisa , Crepaldi Gloria , Data Valeria , Gatto Mariele , Lomater Claudia , Liperoti Gaetano , Marucco Elena , Saracco Marta , Iagnocco Annamaria
Published: June 2024

Background/Objectives: To date, the literature concerning real-world data on the retention rate and safety of Janus kinase inhibitors (JAKis) is limited. To retrospectively evaluate the overall drug retention rate (DRR) of different JAKis in a monocentric cohort of patients with rheumatoid arthritis (RA). Methods: Patients diagnosed with RA and treated with JAKis who were evaluated at our outpatient clinic from March 2017 to December 2023 were included in the study. Demographic, clinical characteristics, and comorbidities were recorded. The DRR was evaluated as the time to drug discontinuation, and baseline predictors of drug discontinuation were investigated through Cox regression after adjusting for baseline confounders. Results: The global DRR for JAKis was 51.3%. The DRR was 37.5% for tofacitinib, 46.6% for baricitinib, 69.4% for upadacitinib, and 53.5% for filgotinib. Considering all JAKis, the only significant predictor of drug discontinuation was the use of JAKis as a first-line treatment (HR 95% CI [0.25 (0.13–0.46)]. When considering each JAKi individually, a longer disease duration predicted TOF discontinuation (HR95% CI [1.05 (1.01–1.09)], while seropositivity protected against TOF being withdrawn (HR95% CI [0.41 (0.17–0.97)]. No independent predictors emerged for other JAKis. Conclusions: the use of JAKis as a first-line treatment as well as disease duration and serology may impact the DRR of JAKis, which may inform tailored treatment strategies in clinical practice.
Does the Layer Configuration of Loofah (Luffa cylindrica) Affect the Mechanical Properties of Polymeric Composites?
Authors: Paula Edgley Alves de Oliveira , Melo Rafael Rodolfo de , Albuquerque Felipe Bento de , Silva Fernanda Monique da , Scatolino Mário Vanoli , Pimenta Alexandre Santos , Paula Edjane Alves de Oliveira , Pedrosa Talita Dantas , Vieira Ricardo Alan da Silva , Rodolfo Junior Francisco
Published: June 2024

The arrangement of layers of natural long fibers that compose a polymeric composite can result in a final material with greater mechanical strength, in addition to replacing synthetic glass and carbon fibers. This study proposed different configurations of layers of loofah fibers (Luffa cylindrica) to produce reinforced polymeric–polyester composites, determining their potential mechanical properties such as flexural strength and Rockwell hardness. The layers were arranged by varying parallel and perpendicularly the direction of the loofah fibers pieces. The reinforcement decreased the density of all composites, with the lowest value, 1.03 g cm−3, indicated by the configuration 90°/0°/90°. The composites in the configuration 0°/90°/0° presented the highest value among the reinforced compositions (10.8 MPa), in addition to the highest rigidity value during bending tests (774.8 MPa). In the Rockwell hardness tests, the treatment reinforced with fibers in the configuration 90°/90°/90° had the highest value among all experimental treatments with a value of 86.9 HHR. The configuration angle of the loofah layers has a significant impact on the mechanical performance of the composites and should be taken into account in their confection. Furthermore, composites reinforced with loofah fibers in different configurations have physical–mechanical properties that qualify them for non-structural applications in indoor environments.
Improving Deposited Surface Quality in Additive Manufacturing Using Structured Light Scanning Characterization and Mechanistic Modeling
Authors: Mukherjee Tuhin , Shen Weijun , Liao Yiliang , Li Beiwen
Published: June 2024

The surface quality of parts fabricated using laser-directed energy deposition additive manufacturing significantly affects the fatigue life, corrosion resistance, and performance of the components. Surface quality improvements remain a key challenge in laser-directed energy deposition because of the involvement of multiple simultaneously occurring physical phenomena controlling the surface characteristics. Here, a unique combination of structured light scanning characterization and mechanistic modeling was used to identify three key physical factors that affect surface quality. These factors include a geometric factor, an instability factor, and a disintegration factor, which were calculated using a mechanistic model and correlated with the surface characteristics data obtained from the structured light scanning characterization. It was found that these factors can precisely explain the variations in the average surface roughness. In addition, skewness and kurtosis of the surfaces made by laser-directed energy deposition were found to be significantly better than those observed in traditional manufacturing. Based on the experimental and modeling results, a surface quality process map was constructed that can guide engineers in selecting appropriate sets of process variables to improve deposit surface quality in additive manufacturing.
The Machining and Surface Modification of H13 Die Steel via the Electrical Discharge Machining Process Using Graphite Mixed Dielectric
Authors: Singh Dharmesh , Goyal Parveen , Sehgal Shankar
Published: June 2024

Surface modification through electrical discharge machining (EDM) results in many advantages, such as improved surface hardness, enhanced wear resistance, and better micro-structuring. During EDM-based surface modification, either the eroding tool electrode or a powder-mixed dielectric can be utilized to add material onto the machined surface of the workpiece. The current study looks at the surface modification of H13 die steel using EDM in a dielectric medium mixed with graphite powder. The experiments were carried out using a Taguchi experimental design. In this work, peak current, pulse-on time, and powder concentration are taken into consideration as input factors. Tool wear rate (TWR), material removal rate (MRR), and the microhardness of the surface of the machined specimen are taken as output parameters. The machined surface’s microhardness was found to have improved by 159%. The results of X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) analysis and changes in MRR and TWR due to the powder-mixed dielectric are also discussed in detail.
Extracts from Microalgae and Archaea from the Andalusian Coast: A Potential Source of Antiproliferative, Antioxidant, and Preventive Compounds
Authors: Luque Cristina , Perazzoli Gloria , Gómez-Villegas Patricia , Vigara Javier , Martínez Rosario , García-Beltrán Alejandro , Porres Jesús M. , Prados Jose , León Rosa , Melguizo Consolación
Published: June 2024

Marine and extreme environments harbor a huge diversity of microorganisms able to produce new bioactive metabolites with beneficial health effects. In this study, ethanol, aqueous, methanol, and acetone extracts and protein hydrolysates were obtained from five different microalgae species and two haloarchaea. An in vitro study of cytotoxicity, migration, angiogenic effect, antioxidant capacity, and modulation of detoxifying enzyme expression was carried out using resistant (HCT-15) and non-resistant (T84) colon cancer tumor lines. Our results showed that the aqueous extract of the microalga Chlorella sorokiniana induced the greatest cytotoxic effect in both cell lines, while the ethanolic extracts of the archaea Haloarcula hispanica and Halobacterium salinarum caused the greatest inhibition on the migratory capacity. Meanwhile, the protein hydrolyzate and the aqueous extract of the microalga Chlorella sorokiniana significantly protected cells against hydrogen peroxide damage. Moreover, the aqueous extracts of Haloarcula hispanica and Halobacterium salinarum resulted in inducing the greatest increase in the activity of the detoxifying enzymes enzyme quinone oxidoreductase and glutathione S-transferase. These preliminary results suggest that aqueous extracts of some microalgae and haloarchaea may be promising candidates for an adjuvant therapy against colorectal cancer. However, additional research is required to identify the active principles and elucidate the mechanisms of action involved.
Comprehensive Characterization of Tuber maculatum, New in Uruguay: Morphological, Molecular, and Aromatic Analyses
Authors: Kuhar Francisco , Tejedor-Calvo Eva , Sequeira Alejandro , Pelissero David , Cosse Mariana , Donnini Domizia , Nouhra Eduardo
Published: June 2024

Although only a few species of Tuber account for the major truffle sales volume, many species that are not considered delicacies are finding their way to the market, especially in regions where the traditionally appreciated ones do not occur. This is the case for whitish truffles. Specimens of whitish truffles were collected in pecan (Carya illinoinensis) orchards in Uruguay in October 2021. Morphological and molecular methods were used to characterize and assess their identity as Tuber maculatum Vittad. An SPME extraction of volatile compounds and GC–MS analyses were performed to characterize the aromatic profile of these specimens and evaluate their potential applications. Among the 60 VOCs detected, 3-octenone (mushroom odor), 3-octanol (moss, nut, mushroom odor), and 2H-pyran-2-one (no odor), followed by octen-1-ol-acetate (no odor) and 2-undecanone (orange, fresh, green odor) were the major compounds in T. maculatum fruiting bodies. The attributes of exotic edible mushrooms of commercial value in the region are highlighted. In particular, this work emphasizes the characteristics of truffles as a byproduct of pecan cultivation.
Neutrophil Oxidative Burst Profile Is Related to a Satisfactory Response to Itraconazole and Clinical Cure in Feline Sporotrichosis
Authors: de Miranda Luisa Helena Monteiro , Santiago Marta de Almeida , Frankenfeld Julia , Reis Erica Guerino dos , Menezes Rodrigo Caldas , Pereira Sandro Antonio , Gremião Isabella Dib Ferreira , Hofmann-Lehmann Regina , Conceição-Silva Fátima
Published: June 2024

Despite the central role of cats in the transmission and amplification of Sporothrix, studies regarding immune response in feline sporotrichosis are scarce. In cats with sporotrichosis, neutrophil-rich lesions are usually associated to good general condition and lower fungal burden. However, the role of neutrophils in anti-Sporothrix immunity has been little explored in cats. Thus, the aim of this study was to evaluate the neutrophil oxidative burst in the blood of cats with sporotrichosis. Cats with sporotrichosis included in the study were treated with itraconazole (ITZ) alone or combined with potassium iodide (KI). The neutrophil oxidative burst was evaluated through a flow-cytometry-based assay using dihydrorhodamine 123 (background) and stimulation with Zymosan and heat-killed Sporothrix yeasts. The cure rate was 50.0% in cats under treatment with ITZ monotherapy and 90.9% in cats treated with ITZ + KI (p = 0.014), endorsing the combination therapy as an excellent alternative for the treatment of feline sporotrichosis. Higher percentages of Sporothrix-stimulated neutrophils were associated with good general condition (p = 0.003). Higher percentages of Sporothrix- (p = 0.05) and Zymosan-activated (p = 0.014) neutrophils before and early in the treatment were related to clinical cure in ITZ-treated cats. The correlation between oxidative burst and successful use of KI could not be properly assessed given the low number of failures (n = 2) in this treatment group. Nasal mucosa involvement, typically linked to treatment failure, was related to lower percentages of activated neutrophils in the background at the treatment outcome (p = 0.02). Our results suggest a beneficial role of neutrophils in feline sporotrichosis and a positive correlation between neutrophil activation and the cure process in ITZ-treated cats.
Morphological and Phylogenetic Analyses Reveal Three New Species of Entomopathogenic Fungi Belonging to Clavicipitaceae (Hypocreales, Ascomycota)
Authors: Wang Zhi-Qin , Ma Jin-Mei , Yang Zhi-Li , Zhao Jing , Yu Zhi-Yong , Li Jian-Hong , Yu Hong
Published: June 2024

This study aims to report three new species of Conoideocrella and Moelleriella from Yunnan Province, Southwestern China. Species of Conoideocrella and Moelleriella parasitize scale insects (Coccidae and Lecaniidae, Hemiptera) and whiteflies (Aleyrodidae, Hemiptera). Based on the phylogenetic analyses of the three-gene nrLSU, tef-1α, and rpb1, it showed one new record species (Conoideocrella tenuis) and one new species (Conoideocrella fenshuilingensis sp. nov.) in the genus Conoideocrella, and two new species, i.e., Moelleriella longzhuensis sp. nov. and Moelleriella jinuoana sp. nov. in the genus Moelleriella. The three new species were each clustered into separate clades that distinguished themselves from one another. All of them were distinguishable from their allied species based on their morphology. Morphological descriptions, illustrations, and comparisons of the allied taxa of the four species are provided in the present paper. In addition, calculations of intraspecific and interspecific genetic distances were performed for Moelleriella and Conoideocrella.
The Impact of Off-Farm Employment Recession and Land on Farmers’ Mental Health: Empirical Evidence from Rural China
Authors: Wang Ziyu , Zhao Qiran , Ji Yueqing
Published: June 2024

The agricultural land management under the household responsibility system (HRS) in rural China empowers farmers with land tenure rights, meeting the basic needs for their livelihoods and employment by cultivating the land. This paper investigates the pivotal role of agricultural cultivated land as a social safety net, ensuring livelihood security for farmers confronting a recession in off-farm employment. Our analysis is based on data collected from six provinces (Heilongjiang, Henan, Zhejiang, Yunnan, Shandong, and Anhui) in the rural areas of China from 2019 to 2020. We investigated the impact of the off-farm employment recession on the mental health of farmers and the moderating effect of land as a social safety net on the relationship between the off-farm employment recession and mental health. The analysis points to the following results: (1) The majority of farmers have significantly reduced off-farm working time during employment recession. (2) The off-farm employment recession has worsened farmers’ mental health. (3) Farmers’ land assets through tenure rights provide both income and employment security functions, which can mitigate the adverse effects of the off-farm employment recession on their mental health. The findings of this study highlight the crucial role of land assets to reduce the negative impact of unemployment in the context of economic recession, emphasizing the significance of the policies supporting the land rights among vulnerable rural groups.
Research on the Value of Water-Related Cultural Heritage Architecture from Historical Environmental Records: Evidence from the Li River Basin in China
Authors: Dai Qifan , Wei Yueqing , Hu Yequan , Chen Tao , Yan Yixun , Gu Yansheng , Wang Qi
Published: June 2024

Water-related cultural heritage architecture (WRCHA) represents a globally significant and potentially hybrid heritage found across river basins worldwide. Its spatial and temporal evolution characteristics offer insight into the development trends of river basin environments, yet their value within water-related cultural and environmental systems remain incompletely assessed. This study undertakes qualitative and quantitative analyses of the historical spatial and temporal distributions, influencing factors, and environmental changes affecting the water-related culture, climate, population, and urban areas of 295 WRCHA sites in the Li River Basin of China, employing drought–flood indices, GIS analyses, random forest algorithms, and other methodologies. The results reveal that (1) the Lishui Basin contains a significant distribution pattern of agglomeration for WRCHA within the river basin, concentrated along the river, at low altitudes, with minimal terrain variation, and radiating around ancient governance centers, with varying increases observed across different periods and aggregation zones and with significant spatial and temporal heterogeneities; (2) the distribution pattern is influenced by joint natural and human factors, closely tied to variables such as the river network density, DEM, population changes, and distance to ancient government sites; (3) the combination of the architecture’s location and elevation, along with drought–flood curves, reflects the position of the ancient riverbed of the Lishui River and its historical maximum water level. The quantity of new constructions, in conjunction with the distance from ancient government centers and the trends in population change, thus indicates the urban scale and the frequency and severity of disasters. This study provides a research paradigm and historical reference model for investigating environmental changes in watershed systems, aiding in clarifying the historical human–water symbiosis pattern in the middle reaches of the Yangtze River. Such insights will furnish a scientific basis for future regional ecological planning and watershed environmental management.
The Impact of and Mechanism behind High-Standard Farmland Construction in Farmland Abandonment: A Moderated Mediating Analysis
Authors: Zhang Yuhan , Zhang Xu , Zhou Wangyue , Li Jianfu , Weng Zhenlin , Gao Xueping
Published: June 2024

At present, farmland abandonment (FA) is a serious problem in China, severely restricting agricultural production. In this context, it is of great significance to explore the logical relationship between high-standard farmland construction (HSFC) and FA to optimize land resource allocation and guarantee national food security. Based on a sample of 838 farmers in the main rice production area of the Yangtze River Basin in China, this study employed the Tobit model, the mediating effect model, and the moderated mediating effect model to analyze the impact of HSFC on FA at the micro level. The results show the following: (1) HSFC inhibits FA and the FA proportion decreases by 1.15% for every 1% increase in the HSFC proportion; the robustness test and endogeneity treatment also yield consistent conclusions. (2) The inhibitory effect of HSFC on FA varies greatly among different farmers and is more significant for part-time farmers and those with a higher degree of land fragmentation. (3) Agricultural socialization services (ASS) play a positive mediating role in the influence path. HSFC promotes the farmers’ purchase of ASS, which in turn inhibits FA. (4) The agricultural labor transfer distance (ALTD) plays a positive moderating role in the relationship between HSFC and FA. The farther the distance, the more likely it is that HSFC can promote the farmers’ purchase of ASS and inhibit FA. The results provide insights regarding how to precisely implement the HSFC policy, i.e., to inhibit FA by improving the construction of high-standard farmland and the post-construction management and protection system. Building targeted construction programs and operational systems that consider the differences in the target groups, improving the standard and capacity of ASS to ensure sustainable benefits for farmers, and promoting the non-agricultural transfer of surplus agricultural labor can create conditions for the modern transformation of the traditional rural economy.
Towards a Comprehensive Framework for Regional Transportation Land Demand Forecasting: Empirical Study from Yangtze River Economic Belt, China
Authors: Wang Ke , Wang Li , Zhang Jianjun
Published: June 2024

China is currently experiencing rapid expansion in its transportation land. To promote sustainable land use, accurately estimating transportation land demand is crucial. This study aims to develop a comprehensive framework for urban transportation land forecasting within the Yangtze River Economic Belt (YREB), providing support for optimizing regional land allocation. Employing methods such as meta-analysis, statistical analysis, and BP neural network analysis, this study forecasts the transportation land demand of 127 cities in the YREB. The study findings indicate that cities with high transportation land demand are mainly distributed in the middle and upper reaches of the Yangtze River. Moreover, the growth rate of transportation land in the upper reaches significantly outstrips that in the middle and lower reaches, suggesting a focus shift in transportation infrastructure construction toward the upper regions. Additionally, some cities within the YREB face a mismatch between the supply and demand of transportation land, necessitating proactive adjustments to their land supply plans to achieve a balance between supply and demand. The main contribution of this study is the development of a comprehensive and adaptable framework that guides the development of future strategies for optimal land allocation by forecasting transportation land demand at a regional level.
A Collaborative Multi-Criteria Decision-Making Framework for the Adaptive Reuse Design of Disused Railways
Authors: Della Spina Lucia , Lanteri Cristina
Published: June 2024

With an increasing focus on sustainable development and the reuse of disused infrastructure, there is a pressing need for effective decision-making methods. This study explored the use of the PROMETHEE method within a collaborative decision-making framework to assess the adaptive reuse options for a disused railway in Sicily, Italy. Using a participatory paradigm, the research actively involves stakeholders in the assessment process. The collaborative PROMETHEE approach acts as a tool for multi-criteria decision analysis, facilitating the comparison and prioritization of different reuse alternatives based on predefined criteria. The research highlights the effectiveness of PROMETHEE in streamlining decision-making processes for adaptive reuse efforts, particularly in the context of integrated conversions of abandoned railways. By combining multi-criteria decision analysis with collaborative methods, the study contributed to the formulation of sustainable and socially responsible strategies for the valorization of disused railway infrastructure. The results underline the importance of collaborative decision-making processes and the instrumental role of PROMETHEE in assisting stakeholders in evaluating and selecting adaptive reuse options for disused railways, thereby, in turn, promoting the sustainable development of these sites.