bluuebunny/crossref_metadata_2025 huggingface dataset:
Spatial and Temporal Distribution of Soil Microbial Properties in Two Shrub Intercrop Systems of the Sahel
Authors: Diedhiou-Sall Sire , Assigbetsee Komi B. , Badiane Aminata N. , Diedhiou Ibrahima , Khouma M. , Dick Richard P.
Published: March 2021

The Sahel is an ecologically vulnerable region where increasing populations with a concurrent increase in agricultural intensity has degraded soils. Agroforestry offers an approach to remediate these landscapes. A largely unrecognized agroforestry resource in the Sahel are the native shrubs, Piliostigma reticulatum, and Guiera senegalensis that to varying degrees already coexist with row crops. These shrubs improve soil quality, redistribute water from the deep soil to the surface (hydraulic lift), and can improve crop growth. However, little information is available on whether these shrubs affect spatial and temporal dynamics of microbial communities. Therefore, the objective of this study was to determine microbial composition and activity in the wet and dry seasons of soil in the: shrub rhizosphere (RhizS), inter-root zone (IntrS), and outside the influence of shrub soil (OutS) for both G. senegalensis and P. reticulatum in Senegal. A 3 × 2 factorial field experiment was imposed at two locations (490 and 700 mm annual rainfall with G. senegalensis and P. reticulatum, respectively), that had the soil sampling treatments of three locations (RhizS, IntrS, and OutS) and two seasons (wet and dry). Soils were analyzed for: microbial diversity (DGGE with bacterial 16S or fungal 28S rRNA gene sequences phospholipids fatty acid, PLFA); enzyme activities; microbial biomass carbon (MBC); and nitrogen (N) mineralization potential. For the DGGE profiling, the bacterial community responded more to the rhizosphere effect, whereas, the fungal community was more sensitive to season. PLFA, MBC, enzyme activities and inorganic N were significantly higher in both seasons for the RhizS. The presence of shrubs maintained rhizosphere microbial communities and activity during the dry season. This represents a paradigm shift for semi-arid environments where logically it would be expected to have no microbial activity in the extended dry season. In contrast this study has shown this is not the case that rather the presence of shrub roots maintained the microbial community in the dry season most likely due to hydraulic lift and root exudates. This has implications when these shrubs are in cropped fields in that decomposition and mineralization of nutrients can proceed in the dry season. Thus, enabling accumulation of plant available nutrients during the dry season for uptake by crops in the rainy season.
Leishmania infantum-Derived Glycoinositolphospholipids in the Immunodiagnosis of Subclinically Infected Dogs
Authors: Sampaio Julia Ramos , Soares Rodrigo Pedro , Barral Thiago Doria , Passos Gabriela Porfirio , Fonseca Maisa Santos , Meyer Roberto , Barrouin-Melo Stella Maria , Portela Ricardo Wagner
Published: March 2021

Lipophosphoglycan (LPG), when used as an ELISA target, confers high specificity and sensitivity to the detection of Leishmania infantum antibodies in dogs. Glycoconjugates are economically viable because the yield is very high after extraction/purification. In addition, they are very stable, which allows their use in point-of-care testing without special storage conditions. During the glycoconjugate extraction, a glycoinositolphospholipid (GIPL)-enriched fraction is obtained in similar quantities as LPG. Since GIPLs can be extracted from the same parasite pellet as LPGs, this work aimed to evaluate the immune recognition of GIPLs by Leishmania infantum-infected dogs and its use for canine leishmaniasis (CanL) immunodiagnosis. Like LPG, GIPLs were recognized by sera from L. infantum-infected dogs, but with less sensitivity (83.8%). However, 80% (16/20) of subclinically infected dogs were detected as positive in the assay. Different from LPG, the GIPL-based assay achieved a lower specificity (73.7%) and cross-reactions occurred with T. cruzi and L. braziliensis-infected dogs. Although GIPLs exhibited a similar performance to LPG for subclinically L. infantum-infected dogs, the occurrence of cross-reactivities with other protozoa and a lower sensitivity hinders its use for an immunodiagnostic test. In places where those diseases do not co-exist such as in the Mediterranean region, its use for subclinically dogs could be an alternative.
The Effects of Feeding Antibiotic on the Intestinal Microbiota of Weanling Pigs
Authors: Lourenco Jeferson M. , Hampton Rachel S. , Johnson Hannah M. , Callaway Todd R. , Rothrock Michael J. , Azain Michael J.
Published: March 2021

This study investigated the use of carbadox in the diet of nursery pigs. Ten pens of weanling piglets were assigned to 2 treatments: one containing carbadox and another without it. From days 21 to 35 of age, the first group of piglets was fed carbadox at 55 mg/kg of diet; followed by 27.5 mg/kg from days 36 to 49; and 0 mg/kg from days 50 to 63. The second group of pigs was fed a control diet without carbadox from days 21 to 63 of age. On days 35, 49, and 63, fecal samples were collected directly from the rectum of 2 piglets in each pen, and the samples were subjected to microbial DNA sequencing and metagenomic functional analysis using the 16S rRNA gene. Feed conversion from days 21 to 63 was improved (P = 0.04) in the group of piglets fed carbadox. Faith's phylogenetic diversity was similar (P = 0.89) for both groups of piglets on day 35, but it was diminished (P = 0.01) in the carbadox-fed group on day 49; however, following the complete removal of carbadox from their diets, this microbial diversity index was once again found to be similar (P = 0.27) in both groups on day 63. Likewise, abundances of Slackia, Peptococcus, Catenibacterium, Coprococcus, and Blautia were all similar between the two groups (P ≥ 0.40) on day 35, but were smaller in the carbadox group (P ≤ 0.05) on day 49; however, on day 63, abundances of all these genera were once again similar (P ≥ 0.29). Metabolic pathways involved in cellular growth, death, and genetic information processing (translation) were found to be similarly expressed in the microbiota of piglets from both groups on day 35 (P ≥ 0.52), but decreased in the carbadox group on day 49 (P ≤ 0.05), and were similar again in both groups on day 63 (P ≥ 0.51). These results revealed that feeding carbadox to piglets during the first 4 weeks after weaning significantly affected their fecal microbiotas; however, 2 weeks after the removal of carbadox, those changes tended to disappear, indicating that the shifts were carbadox-dependent.
Case Report: A Possible Case of Congenital Erythropoietic Porphyria in a Gir Calf: A Clinical, Pathological, and Molecular Approach
Authors: Queiroz Cintia Regina Rêgo , Machado Mizael , Bromberger Cristiana Raach , Oliveira-Filho Jose Paes , Borges Alexandre Secorun , Soto-Blanco Benito , Borges José Renato Junqueira , Câmara Antônio Carlos Lopes , Castro Márcio Botelho de
Published: March 2021

Congenital erythropoietic porphyria (CEP) is a rare hereditary autosomal recessive disease which has never been reported in Zebu cattle. A 3-day-old Gir calf showed teeth discoloration, fever, dehydration, and dyspnea. The main gross findings were pink-colored teeth, red-brown periosteum and bone marrow, and a fluorescent bright pink coloration of the bone marrow and articular surfaces under ultraviolet light. Aggregates of periodic acid-Schiff (PAS)-stained porphyrin pigments were evident in the lungs, kidneys, and the liver. An intron 8 single-nucleotide polymorphism (SNP) in both the Gir calf and control animals, along with the absence of the uroporphyrin III synthetase (UROS) gene mutation, was observed. Most SNPs were located in the intron regions of the UROS gene without relevance for CEP. A continuous loss of genetic variability and an increase in inbreeding in some herds may be related to CEP in Gir cattle, one of the most prominent Zebu breeds worldwide. In summary, this study describes a presumptive case of CEP in a Gir calf based on clinical and pathological findings. A definitive diagnosis would require the measurement of porphyrin levels in blood, urine, or tissues or the identification of UROS gene defects.
Repeated Evaluations of Testes and Semen Characteristics in Two Binturongs (Arctictis binturong)
Authors: Zainuddin Zainal Zahari , Sipangkui Symphorosa , Farqhan Kelana Mohd , Chee Yap Keng , Tarmizi Mohamed Reza Mohamed , Comizzoli Pierre
Published: March 2021

The binturong is a medium size carnivore belonging to the Viverrid family that lives in dense forests of South-East Asia. In addition to the protection of this vulnerable species in its natural habitat (in situ), conservation breeding efforts (ex situ) aim at maintaining a good genetic diversity while increasing the number of individuals to reinforce wild populations. Both approaches require a solid understanding of binturong's basic biology. However, there is still a lack of precise information about reproduction. The objective of this brief research report was to analyze testicular sizes and semen characteristics at different times of the year to better understand the reproductive physiology and inform future conservation efforts. A secondary objective was to describe sperm cryotolerance for the first time in that species. Examinations of testes and semen collections were conducted on two adult males. While testicular measurements were relatively constant across multiple examinations, semen characteristics (volume, viability, sperm concentrations, sperm motility) varied between samples. However, incidence of sperm morphological abnormalities was consistently high. Sperm cryotolerance appeared to be poor but further studies are warranted. The present dataset will be useful for future research on binturong reproduction and for the development of assisted reproductive techniques and biobanking of germplasms in that species.
Correction: Razgon, M., et al. Relaxed Rule-Based Learning for Automated Predictive Maintenance: Proof of Concept. Algorithms 2020, 13, 219
Authors: Razgon Margarita , Mousavi Alireza
Published: March 2021

The authors wish to make the following corrections to their paper [...]
Innovation Gaps and Barriers in Alternative Innovative Solutions for Sustainable High Nature Value Grasslands. Evidence from Romania
Authors: Jitea Mugurel I. , Mihai Valentin C. , Arion Felix H. , Muresan Iulia C. , Dumitras Diana E.
Published: March 2021

Common Agricultural Policy (CAP) proposes environmental policies developed around action-based conservation measures supported by agri-environment schemes (AES). High Nature Value (HNV) farming represents a combination of low-intensity and mosaic practices mostly developed in agricultural marginalized rural areas which sustain rich biodiversity. Being threatened by intensification and abandonment, such farming practices were supported in the last CAP periods by targeted AES. This study aims to identify the most important farming trends in a recognized HNV grasslands Romanian site, subject to public conservation actions. The content analysis of multi-actor focus groups transcripts identifies innovation needs for alternative sustainable HNV farming systems, assessing also the most important barriers for their local deployment. Results show that stakeholders have strong awareness about the existence of HNV farming practices in their territory and their negative trends (abandonment; intensification) that might irreversibly affect biodiversity and cultural values. Current AES could not compensate for the existing innovation gaps. Although local stakeholders have basic awareness about alternative innovative approaches, they failed in their deployment mainly due to the lack of collective actions. Access to adapted knowledge reservoirs and the promotion of local innovation catalyzers are essential for the future sustainability of HNV farming practices.
In-Line Technologies for the Analysis of Important Milk Parameters during the Milking Process: A Review
Authors: Kunes Radim , Bartos Petr , Iwasaka Gustavo Kenji , Lang Ales , Hankovec Tomas , Smutny Lubos , Cerny Pavel , Poborska Anna , Smetana Pavel , Kriz Pavel , Kernerova Nadezda
Published: March 2021

Considering automatized and robotic milking systems substantially decreasing the contact between producers and the herd, milk analysis is crucial to maintain the quality and safety of all dairy products. These systems naturally also decrease the possibility of health problems and illness identification. Abnormalities in milk can be caused by several factors. Milk quality can be affected by external conditions, such as temperature and contamination in the feedstock; by management practices, such as hygiene, milking frequency, treatment, and feedstuff quality; and by diseases, genetics, or age. Somatic cell count, electric conductivity, and contents of urea, fat, protein, and lactose were reviewed as likely parameters of milk representing its quality with respect to feedback for consumers and breeders. Methods for evaluating milk constituents and parameters are still being developed to provide in-line information. These methods allow the avoidance of enormous economic losses every year caused by milk discard, health treatments, or cow replacements. In addition, individual and in-line milk analysis provides information in terms of nutritional status or lactation period and fertility. The objective of this study is to identify trends and potential methods focusing on in situ and in-line techniques for the analysis of milk parameters during the automatized and robotic milking process. Four methods are described and compared: near-infrared spectroscopy (NIRS) and mid-infrared spectroscopy (MIRS), optical analysis, milk conductivity analysis, and milk leukocyte differential test. The versatility and accessibility of these methods were also evaluated, showing a considerable range of possible related problems.
Evaluating the Financial Health of Agricultural Enterprises in the Conditions of the Slovak Republic Using Bankruptcy Models
Authors: Vavrek Roman , Kravčáková Vozárová Ivana , Kotulič Rastislav
Published: March 2021

Under the influence of the change in the overall economic environment, the problem of measuring the performance of a company and its financial health is also changing. At present, every agricultural company is exposed to a number of internal as well as external risks, the failure of which can lead to potential bankruptcy. It is a known fact that the risks in agriculture are significantly greater than those in other sectors of the national economy. Proper diagnosis of critical aspects and measuring the development of individual financial indicators of agricultural holdings are basic prerequisites for eliminating these risks and maintaining, as well as increasing, their competitiveness. Among the key tools for measuring the financial situation of a company are bankruptcy models, three of which have been used (Altman model, Taffler model, and Bonity index). The aim of this study was to identify the comprehensive financial health of 469 agricultural enterprises in the Slovak Republic using the three above-mentioned bankruptcy models in 2016. The obtained results were verified using the Kruskal–Wallis test, Levene test, or Moran index. Altman’s model indicated potential future problems of businesses and agricultural cooperatives. Using the Bonity index, the neutral situation was assessed (the financial health could be regarded as neither insufficient nor optimal). Taffler model offered contradicted results and does not expect the occurrence of problems soon. In the study, we also verified the assumption of the dependence of financial health of companies on the legal form and territorial division, which was confirmed to be insignificant.
Weed Diversity, Abundance, and Seedbank in Differently Tilled Faba Bean (Vicia faba L.) Cultivations
Authors: Romaneckas Kęstutis , Kimbirauskienė Rasa , Sinkevičienė Aušra , Jaskulska Iwona , Buragienė Sidona , Adamavičienė Aida , Šarauskis Egidijus
Published: March 2021

Differently tilled faba bean cultivations, in particular, require a comprehensive study of weed diversity, abundance, and seedbank due to the lack of experimental data. Therefore, in 2016–2018, field trials were conducted at Vytautas Magnus University on the basis of a long-term tillage experiment. Conventional deep and shallow plowing, deep chiseling, shallow disking, and no-tillage systems were investigated. According to the results of the investigations, the air temperature and amount of precipitation during the vegetative season had a greater influence on the total number of weeds (r = 0.538 and 0.833 p > 0.05) than the types of tillage systems investigated. However, on average, a reduction in tillage intensity did not change the weed number, especially in disked and not tilled plots. On average, the biomass of weeds varied little between the treatments (from 105.9 to 125.7 g m−2) and mainly depended on the volume of forecrop residues (rannual = −0.982 p ≤ 0.01 and rperennial = 0.890 p ≤ 0.05). Higher total weed seedbanks were found in the disked (+43.0%) and not tilled (+21.6%) soils compared to deeply plowed ones. The weed seedbank was almost similarly distributed between the treatments, irrespective of the tillage depth and method used.
Effects of Light Quality Treatments during the Grain Filling Period on Yield, Quality, and Fragrance in Fragrant Rice
Authors: Xie Huijia , Xie Wenjun , Pan Shenggang , Liu Xuwei , Tian Hua , Duan Meiyang , Wang Shuli , Tang Xiangru , Mo Zhaowen
Published: March 2021

The effect of the light quality on 2-acetyl-1-pyrroline (2AP) during the grain filling period in fragrant rice has rarely been investigated. A pot experiment was carried out with two fragrant rice varieties, Xiangyaxiangzhan and Yuxiangyouzhan, grown under three light treatments, 100% red light (L1), 100% blue light (L2), and compound light (L3), during the grain filling period, and natural light was taken as the control (CK). The yield, quality, and fragrance were investigated. The results showed that light quality treatments significantly decreased the 2AP content in mature grains by 16.67–32.82% but improved the grain yield by 2.70–21.41% compared to CK. The regulation effects of light quality treatments on grain yield and 2AP are linked to yield-related traits, biomass accumulation, antioxidant physiology, and 2AP formation-related physiology. Additionally, light quality treatments decreased the chalky rice percentage and chalkiness, and increased the length-to-width ratio. Overall, light quality treatments during the grain filling period had a positive effect on the grain yield but not on fragrance in fragrant rice.
Machine-Learning Approach Using SAR Data for the Classification of Oil Palm Trees That Are Non-Infected and Infected with the Basal Stem Rot Disease
Authors: Hashim Izrahayu Che , Shariff Abdul Rashid Mohamed , Bejo Siti Khairunniza , Muharam Farrah Melissa , Ahmad Khairulmazmi
Published: March 2021

Basal stem rot disease (BSR) in oil palm plants is caused by the Ganoderma boninense (G. boninense) fungus. BSR is a major disease that affects oil palm plantations in Malaysia and Indonesia. As of now, the only available sustaining measure is to prolong the life of oil palm trees since there has been no effective treatment for the BSR disease. This project used an ALOS PALSAR-2 image with dual polarization, Horizontal transmit and Horizontal receive (HH) and Horizontal transmit and Vertical receive (HV). The aims of this study were to (1) identify the potential backscatter variables; and (2) examine the performance of machine learning (ML) classifiers (Multilayer Perceptron (MLP) and Random Forest (RF) to classify oil palm trees that are non-infected and infected by G. boninense. The sample size consisted of 55 uninfected trees and 37 infected trees. We used the imbalance data approach (Synthetic Minority Over-Sampling Technique (SMOTE) in these classifications due to the differing sample sizes. The result showed backscatter variable HV had a higher correct classification for the G. boninense non-infected and infected oil palm trees for both classifiers; the MLP classifier model had a robust success rate, which correctly classified 100% for non-infected and 91.30% for infected G. boninense, and RF had a robust success rate, which correctly classified 94.11% for non-infected and 91.30% for infected G. boninense. In terms of model performance using the most significant variables, HV, the MLP model had a balanced accuracy (BCR) of 95.65% compared to 92.70% for the RF model. Comparison between the MLP model and RF model for the receiver operating characteristics (ROC) curve region, (AUC) gave a value of 0.92 and 0.95, respectively, for the MLP and RF models. Therefore, it can be concluded by using only the HV polarization, that both the MLP and RF can be used to predict BSR disease with a relatively high accuracy.
Agrotechnical Biofortification as a Method to Increase Selenium Content in Spring Wheat
Authors: Radawiec Aleksandra , Szulc Wiesław , Rutkowska Beata
Published: March 2021

Selenium (Se) is a micronutrient that is insufficiently present in the human diet. Increasing its content in food through appropriately matched agricultural practices may contribute to reducing Se deficit in humans. The study covered the effect of grain, soil, as well as grain and soil fertilization with selenium combined with foliar application at different stages of spring wheat (Triticum aestivum L.) development. The fertilization involved the application of sodium selenate. Fertilization with selenium had no significant effect on the grain yield. Grain application, soil application, and grain and soil application combined with foliar application at particular development stages of the plant significantly contributed to an increase in selenium content in grain. The study showed that the accumulation of selenium in spring wheat depends on the type of fertilization and term of its application. The best method of introducing selenium into the plant is grain and soil fertilization combined with foliar application at the stage of tillering and stem elongation (G + S + F1-2) for which the highest selenium content was obtained (0.696 mg·kg−1 Se). The applied biofortification methods contributed to the increase in selenium in the grain of spring wheat.
Changes Caused by Low Doses of Bisphenol A (BPA) in the Neuro-Chemistry of Nerves Located in the Porcine Heart
Authors: Makowska Krystyna , Gonkowski Slawomir
Published: March 2021

Bisphenol A (BPA) contained in plastics used in the production of various everyday objects may leach from these items and contaminate food, water and air. As an endocrine disruptor, BPA negatively affects many internal organs and systems. Exposure to BPA also contributes to heart and cardiovascular system dysfunction, but many aspects connected with this activity remain unknown. Therefore, this study aimed to investigate the impact of BPA in a dose of 0.05 mg/kg body weight/day (in many countries such a dose is regarded as a tolerable daily intake–TDI dose of BPA–completely safe for living organisms) on the neurochemical characterization of nerves located in the heart wall using the immunofluorescence technique. The obtained results indicate that BPA (even in such a relatively low dose) increases the number of nerves immunoreactive to neuropeptide Y, substance P and tyrosine hydroxylase (used here as a marker of sympathetic innervation). However, BPA did not change the number of nerves immunoreactive to vesicular acetylcholine transporter (used here as a marker of cholinergic structures). These observations suggest that changes in the heart innervation may be at the root of BPA-induced circulatory disturbances, as well as arrhythmogenic and/or proinflammatory effects of this endocrine disruptor. Moreover, changes in the neurochemical characterization of nerves in the heart wall may be the first sign of exposure to BPA.
Mechanical Processing of Hermetia illucens Larvae and Bombyx mori Pupae Produces Oils with Antimicrobial Activity
Authors: Saviane Alessio , Tassoni Luca , Naviglio Daniele , Lupi Daniela , Savoldelli Sara , Bianchi Giulia , Cortellino Giovanna , Bondioli Paolo , Folegatti Liliana , Casartelli Morena , Orlandi Viviana Teresa , Tettamanti Gianluca , Cappellozza Silvia
Published: March 2021

The aim of this work was to develop processing methods that safeguard the quality and antimicrobial properties of H. illucens and B. mori oils. We adopted a vegetable diet for both insects: leftover vegetables and fruit for H. illucens and mulberry leaves for B. mori. First, alternative techniques to obtain a good oil extraction yield from the dried biomass of H. illucens larvae were tested. Traditional pressing resulted to be the best system to maximize the oil yield and it was successfully applied to B. mori pupae. Oil quality resulted comparable to that obtained with other extraction methods described in the literature. In the case of B. mori pupae, different treatments and preservation periods were investigated to evaluate their influence on the oil composition and quality. Interestingly, agar diffusion assays demonstrated the sensitivity of Gram-positive Bacillus subtilis and Staphylococcus aureus to H. illucens and B. mori derived oils, whereas the growth of Gram-negative Pseudomonas aeruginosa and Escherichia coli was not affected. This study confirms that fat and other active compounds of the oil extracted by hot pressing could represent effective antimicrobials against bacteria, a relevant result if we consider that they are by-products of the protein extraction process in the feed industry.
Leptospira Seroprevalence in Colombian Dairy Herds
Authors: Taddei Simone , Moreno Giovanni , Cabassi Clotilde Silvia , Schiano Emiliana , Spadini Costanza , Cavirani Sandro
Published: March 2021

Leptospirosis in cattle has important economic effects on the infected farms. Moreover, livestock farming is considered a major occupational risk factor for the transmission of Leptospira infection to humans. A survey was performed to determine the overall and within-herd seroprevalence and mapping of different Leptospira serovars in dairy cattle from farms located in some municipalities of the Colombian department of Boyacá. Nine hundred and fifty-nine animals, from 20 unvaccinated and one vaccinated herd, were included in the study. Anti-Leptospira serum antibodies were detected by the microscopic agglutination test (MAT). Only one herd was seronegative. Overall seroprevalence to at least one serovar of Leptospira was 24.1% for unvaccinated animals and 62.3% for animals from the vaccinated herd. A very high within-herd seroprevalence (>60%) was present in 20% of the unvaccinated herds. The presence in the vaccinated herd of 20/398 animals showing high titers, between 1000 and 4000, to at least one serovar of Leptospira suggest that some animals could have been infected. Moreover, due to the presence of seronegative animals, a failure of vaccination immunity or the presence of unvaccinated animals in the vaccinated herd cannot be excluded. In all farms, domestic animals other than cattle were present. Considering the farming practices occurring on dairy farms in the study area, higher hygienic standards and stricter biosecurity measures are suggested.
Activity of Mannose-Binding Lectin on Bacterial-Infected Chickens—A Review
Authors: Idowu Peter A. , Idowu Adeola P. , Zishiri Oliver T. , Mpofu Takalani J. , Veldhuizen Edwin J. A. , Nephawe Khathutshelo A. , Mtileni Bohani
Published: March 2021

In recent years, diseases caused by pathogenic bacteria have profoundly impacted chicken production by causing economic loss in chicken products and by-product revenues. MBL (mannose-binding lectin) is part of the innate immune system (IIS), which is the host’s first line defense against pathogens. The IIS functions centrally by identifying pathogen-specific microorganism-associated molecular patterns (MAMPs) with the help of pattern recognition receptors (PRRs). Studies have classified mannose-binding lectin (MBL) as one of the PRR molecules which belong to the C-type lectin family. The protective role of MBL lies in its ability to activate the complement system via the lectin pathway and there seems to be a direct link between the chicken’s health status and the MBL concentration in the serum. Several methods have been used to detect the presence, the level and the structure of MBL in chickens such as Enzyme-linked immunosorbent assay (ELISA), Polymerase Chain Reaction (PCR) among others. The concentration of MBL in the chicken ranges from 0.4 to 35 µg/mL and can be at peak levels at three to nine days at entry of pathogens. The variations observed are known to depend on the bacterial strains, breed and age of the chicken and possibly the feed manipulation strategies. However, when chicken MBL (cMBL) becomes deficient, it can result in malfunctioning of the innate immune system, which can predispose chickens to diseases. This article aimed to discuss the importance and components of mannose-binding lectin (MBL) in chickens, its mode of actions, and the different methods used to detect MBL. Therefore, more studies are recommended to explore the causes for low and high cMBL production in chicken breeds and the possible effect of feed manipulation strategies in enhancing cMBL production.
Addendum: Gonzalez, J.J., et al. Automatically Detected Pecking Activity in Group-Housed Turkeys. Animals 2020, 10, 2034
Authors: Gonzalez Jennifer J. , Nasirahmadi Abozar , Knierim Ute
Published: March 2021

The authors wish to add the following to this paper [...]
Reproductive Toxicity of 3,4-dichloroaniline (3,4-DCA) on Javanese Medaka (Oryziasjavanicus, Bleeker 1854)
Authors: Ibrahim Musa Adamu , Zulkifli Syaizwan Zahmir , Azmai Mohammad Noor Amal , Mohamat-Yusuff Ferdaus , Ismail Ahmad
Published: March 2021

Compound 3,4-dichloroaniline (3,4-DCA) is a metabolite of several urea herbicides and intermediate chemical of several industrial products. Moreover, 3,4-DCA has been frequently detected in aquatic ecosystems around the world. This aniline is more toxic than the parent chemicals, and it affects non-target organisms. This study evaluated a 21-day reproductive response of an emerging aquatic vertebrate model, Javanese medaka (Oryzias javanicus), exposed to 3,4-DCA. Fecundity and gonads histopathology were observed. The spawning rate and fertilisation reduced significantly in the highest exposed-group (250 µg/L). Gonadosomatic index (GSI) was significantly low in females exposed to 250 µg/L. No substantial structural alteration of male gonads. However, oocyte development and ovarian cell structure were disrupted in 250 µg/L exposed females. The gonadal developmental was not affected in the males; however, a significant reduction in the developmental of female gonads was observed at 250 µg/L. These results show that 3,4-DCA interfere with the reproduction of Javanese medaka through fecundity and alteration of gonadal tissues.
Cryptosporidium spp. and Giardia spp. in Free-Ranging Introduced Monk Parakeets from Santiago, Chile
Authors: Sandoval-Rodríguez Alejandra , Marcone Daniela , Alegría-Morán Raúl , Larraechea Matilde , Yévenes Karina , Fredes Fernando , Briceño Cristóbal
Published: March 2021

Monk Parakeets (Myiopsitta monachus) are medium-sized parrots that due to international pet trade currently exist as invasive species in 19 countries globally. Such is the case of Chile, where Monk Parakeets have thrived in the city of Santiago. Cryptosporidium spp. and Giardia spp. are worldwide distributed gastrointestinal parasites whose potential hosts include birds and humans. The present study sought to determine the presence of these pathogens in Monk Parakeets from Santiago. During the austral summers of 2017 and 2018, 207 Monk Parakeet nestlings were captured, and fecal samples were studied via microscopical analyses. Environmental data related to the trees in which the nestlings were captured were analyzed to establish the existence of infection clusters. Associations between spatial clusters, environmental variables, and the presence or absence of these pathogens were explored. In total, 33 samples were positive to the presence of one or both protozoa. Of the 33, Cryptosporidium spp. oocysts were detected in 10 nestlings (30%) while Giardia spp. cysts were detected in 25 (76%). Two nestlings presented poly-parasitism (6%). Statistical analyses established pruned trees as a potential protective factor against infection with these parasites. The present study corresponds to the second report of Cryptosporidium spp. in Monk Parakeets in Chile and the first worldwide report of Giardia spp. in these birds, emphasizing Monk Parakeet’s potential role as a reservoir and pathogen disseminator, especially in urban environments.
Laser Use in Creating Orthodontic Adhesion to Ceramic Surfaces
Authors: Labunet Anca , Kui Andreea , Sava Sorina
Published: March 2021

Orthodontists must sometimes bond attachments to ceramic crowns, by using one of the surface preparations available, such as sandblasting or acid etching with hydrofluoric acid. Research shows that different laser systems may also be used for this purpose. The aim of this review is to determine which laser type and modality of use is the most effective in increasing shear bond strength of brackets bonded to ceramic surfaces. Two independent researchers studied the current literature 1990–2018 and selected original articles focusing on in vitro research on laser use for ceramic surface preparation for bonding metallic or ceramic attachments. Twelve articles have met the criteria and have been thoroughly revised, focusing on 2 fractional, Nd:Yag, Er:Yag, femtosecond and Ti:Sapphire lasers. There is little difference shown by the current studies between ceramic types and information on orthodontic bonding to non-feldspathic ceramics is scarce. Femtosecond laser is a good alternative to classical surface preparation with hydrofluoric acid. Nd:Yag laser is more suitable for surface preparation of ceramics than different types of Er:Yag lasers. Difference in laser power may achieve different results, but the golden standard for lasers use has not been found. Laser usage does not obtain the same results as hydrofluoric acid and cannot, so far, eliminate its application.
A Comparative Study of Energy Consumption and Recovery of Autonomous Fuel-Cell Hydrogen–Electric Vehicles Using Different Powertrains Based on Regenerative Braking and Electronic Stability Control System
Authors: Yildiz Ahmet , Özel Mert Ali
Published: March 2021

Today, with the increasing transition to electric vehicles (EVs), the design of highly energy-efficient vehicle architectures has taken precedence for many car manufacturers. To this end, the energy consumption and recovery rates of different powertrain vehicle architectures need to be investigated comprehensively. In this study, six different powertrain architectures—four independent in-wheel motors with regenerative electronic stability control (RESC) and without an RESC, one-stage gear (1G) transmission, two-stage gear (2G) transmission, continuously variable transmission (CVT) and downsized electric motor with CVT—were mathematically modeled and analyzed under real road conditions using nonlinear models of an autonomous hydrogen fuel-cell electric vehicle (HFCEV). The aims of this paper were twofold: first, to compare the energy consumption performance of powertrain architectures by analyzing the effects of the regenerative electronic stability control (RESC) system, and secondly, to investigate the usability of a downsized electrical motor for an HFCEV. For this purpose, all the numerical simulations were conducted for the well-known FTP75 and NEDC urban drive cycles. The obtained results demonstrate that the minimum energy consumption can be achieved by a 2G-based powertrain using the same motor; however, when an RESC system is used, the energy recovery/consumption rate can be increased. Moreover, the results of the article show that it is possible to use a downsized electric motor due to the CVT, and this powertrain significantly reduces the energy consumption of the HFCEV as compared to all the other systems. The results of this paper present highly significant implications for automotive manufacturers for designing and developing a cleaner electrical vehicle energy consumption and recovery system.
Multi-Manned Assembly Line Balancing: Workforce Synchronization for Big Data Sets through Simulated Annealing
Authors: Pilati Francesco , Ferrari Emilio , Gamberi Mauro , Margelli Silvia
Published: March 2021

The assembly of large and complex products such as cars, trucks, and white goods typically involves a huge amount of production resources such as workers, pieces of equipment, and layout areas. In this context, multi-manned workstations commonly characterize these assembly lines. The simultaneous operators’ activity in the same assembly station suggests considering compatibility/incompatibility between the different mounting positions, equipment sharing, and worker cooperation. The management of all these aspects significantly increases the balancing problem complexity due to the determination of the start/end times of each task. This paper proposes a new mixed-integer programming model to simultaneously optimize the line efficiency, the line length, and the workload smoothness. A customized procedure based on a simulated annealing algorithm is developed to effectively solve this problem. The aforementioned procedure is applied to the balancing of the real assembly line of European sports car manufacturers distinguished by 665 tasks and numerous synchronization constraints. The experimental results present remarkable performances obtained by the proposed procedure both in terms of solution quality and computation time. The proposed approach is the practical reference for efficient multi-manned assembly line design, task assignment, equipment allocation, and mounting position management in the considered industrial fields.
An In-Line Coaxial-to-Waveguide Transition for Q-Band Single-Feed-Per-Beam Antenna Systems
Authors: Simone Marco , Fanti Alessandro , Lodi Matteo Bruno , Pisanu Tonino , Mazzarella Giuseppe
Published: March 2021

An in-line transition between a coaxial cable and rectangular waveguide operating in Q-band (33–50 GHz) is presented. The aim of the work is to minimize the modifications in the waveguide to the strictly necessary to overcome the manufacturing issues due to the high frequencies involved. In addition, the transition is compact and it does not increase the space occupation on the transverse section, this suggests its application in horn antennas clusters arrangement. The operating principle consists of both a modal conversion and an impedance matching between the devices. The modal conversion is realized in an intermediate region, where the coaxial penetrates in the waveguide: the device geometry is designed so that the electric field in the transition is a trade-off between the TEM mode of the coaxial and the TE10 of the guide. A shaped waveguide backshort and a reactive air gap in the coaxial cable co-participate to achieve the matching. An optimized Chebyshev stepped transformer completes the transition to fulfil the impedance mismatch with the full waveguide. The design issues and technological aspects are considered. The influences of the feeding pin misalignment, the presence of groove is included in the analysis and these practical aspects are discussed and numerically validated via the scattering parameters analysis of the proposed design. The return loss is higher than 25 dB over the whole Q-band.
Evaluation of the Nasolabial Angle in Orthodontic Diagnosis: A Systematic Review
Authors: Quinzi Vincenzo , Paskay Licia Coceani , D’Andrea Nicola , Albani Arianna , Monaco Annalisa , Saccomanno Sabina
Published: March 2021

Background: This study is a systematic literature review aiming at identifying the variation of the average nasolabial angle (NLA) in various orthodontic situations. The NLA is one of the key factors to be studied in an orthodontic diagnosis for the aesthetics of the nose and facial profile. Methods: Out of 3118 articles resulting from four search engines (PubMed, Cochrane Library, Turning Research Into Practice (TRIP) and SciELO), the final study allowed the analysis and comparison of only 26 studies. These included studies have considered the NLA in the following cases: teeth extraction, class II malocclusion, class III malocclusion, rapid palatal expansion (RPE), orthognathic surgery, and non-surgical rhinoplasty with a hyaluronic acid filler. Results: The results indicate that teeth extraction and the use of hyaluronic acid fillers significantly affect the NLA. Conclusions: This systematic review shows that a statistically significant change in NLA values occurs in: extractive treatments of all four of the first or second premolars in class I patients; in class II patients with upper maxillary protrusion; in patients with maxillary biprotrusion, except for cases of severe crowding; and in patients undergoing non-surgical rhinoplasty with a hyaluronic acid filler. Trial registration number: PROSPERO CRD42020185166
Plasma Treatment of Fish Cells: The Importance of Defining Cell Culture Conditions in Comparative Studies
Authors: Rebl Henrike , Bergemann Claudia , Rakers Sebastian , Nebe Barbara , Rebl Alexander
Published: March 2021

The present study provides the fundamental results for the treatment of marine organisms with cold atmospheric pressure plasma. In farmed fish, skin lesions may occur as a result of intensive fish farming. Cold atmospheric plasma offers promising medical potential in wound healing processes. Since the underlying plasma-mediated mechanisms at the physical and cellular level are yet to be fully understood, we investigated the sensitivity of three fish cell lines to plasma treatment in comparison with mammalian cells. We varied (I) cell density, (II) culture medium, and (III) pyruvate concentration in the medium as experimental parameters. Depending on the experimental setup, the plasma treatment affected the viability of the different cell lines to varying degrees. We conclude that it is mandatory to use similar cell densities and an identical medium, or at least a medium with identical antioxidant capacity, when studying plasma effects on different cell lines. Altogether, fish cells showed a higher sensitivity towards plasma treatment than mammalian cells in most of our setups. These results should increase the understanding of the future treatment of fish.
PAM-N—Fundamental Limits in Chromatic Dispersive-Uncompensated Channels
Authors: Granot Er’el , Katz Gilad
Published: March 2021

The fundamental chromatic dispersion limit for an optical communication N-level pulse amplitude modulation (PAM-N) format without any dispersion compensating module is calculated. The main result of this analysis shows that in a non-dispersion-compensated channel, the product β2B2L (where β2, L, and B are the dispersion coefficient, fiber length, and the Baud rate, respectively) is bounded by a number, which depends only on the number of levels N. In particular, β2B2L < 0.318, β2B2L < 0.212, and β2B2L < 0.14 for N values of 2, 4, and 8, respectively. Moreover, an analytical expression for a noisy channel’s power penalty was formulated. This analytic expression shows high agreement with numerical simulations. To the best of our knowledge, this is the first time that such a fundamental limit has been formulated for PAM-N systems.
Strengthening Effect of the External Prestressing Method That Simulated a Deterioration Bridge
Authors: Kim Sang-Hyun , Park Jong-Sup , Jung Woo-Tai , Kang Jae-Yoon
Published: March 2021

Various methods for strengthening existing structures have been developed owing to the increase in human and property damages caused by the deterioration of structures. Among the various reinforcing methods, the external prestressing method increases the usability and safety of a structure by directly applying tension to the weak tensile area that suffers the greatest deflection during the structure usage. The external prestressing method is advantageous in reducing cracks caused by the introduced tension and restoration of the deflection. Since the strengthening method is applied to deterioration bridges, the strengthening effect is affected by the condition of the existing structure. However, studies on the strengthening effect according to the degree of deterioration are insufficient. Therefore, the behavior according to the strengthening status was analyzed, and the strengthening effect was identified in this study by simulating the deteriorated bridge, reducing the compressive strength and reinforcement quantity, and conducting a four-point loading test. As a result of the experiment, a reinforcement effect of 215% crack load, 161% yield load, and the difference in behavior according to the reinforcement parameters were confirmed.
Implementation of Machine Learning Algorithms in Spectral Analysis of Surface Waves (SASW) Inversion
Authors: Mitu Sadia Mannan , Rahman Norinah Abd. , Nayan Khairul Anuar Mohd , Zulkifley Mohd Asyraf , Rosyidi Sri Atmaja P.
Published: March 2021

One of the complex processes in spectral analysis of surface waves (SASW) data analysis is the inversion procedure. An initial soil profile needs to be assumed at the beginning of the inversion analysis, which involves calculating the theoretical dispersion curve. If the assumption of the starting soil profile model is not reasonably close, the iteration process might lead to nonconvergence or take too long to be converged. Automating the inversion procedure will allow us to evaluate the soil stiffness properties conveniently and rapidly by means of the SASW method. Multilayer perceptron (MLP), random forest (RF), support vector regression (SVR), and linear regression (LR) algorithms were implemented in order to automate the inversion. For this purpose, the dispersion curves obtained from 50 field tests were used as input data for all of the algorithms. The results illustrated that SVR algorithms could potentially be used to estimate the shear wave velocity of soil.
Bandwidth Improvement of an Inverted-F Antenna Using Dynamic Hybrid Binary Particle Swarm Optimization
Authors: Alnas Jude , Giddings Garrett , Jeong Nathan
Published: March 2021

This paper proposes a Dynamic Hybrid Binary Particle Swarm Optimization (DH-BPSO) algorithm to improve the bandwidth of an inverted-F antenna (IFA). The proposed algorithm improves upon the existing Artificial Immune System (AIS) algorithm by including a weighting factor that dynamically changes throughout the optimization. DH-BPSO activates or deactivates a 12 × 2 grid of parasitic patches incorporated between the IFA and ground plane. The DH-BPSO optimized and conventional IFAs are fabricated and compared while maintaining the same antenna volume. The measurement results show that the optimized IFAs have characteristics of 58.6% wider bandwidths and 5.8% higher antenna gain for various ground clearance lengths at Long Term Evolution (LTE) 700 MHz band compared to the conventional IFAs.
Optimization of Electron Beams Based on Plasma-Density Modulation in a Laser-Driven Wakefield Accelerator
Authors: Ke Lintong , Yu Changhai , Feng Ke , Qin Zhiyong , Jiang Kangnan , Wang Hao , Luan Shixia , Yang Xiaojun , Xu Yi , Leng Yuxin , Wang Wentao , Liu Jiansheng , Li Ruxin
Published: March 2021

We demonstrate a simple but efficient way to optimize and improve the properties of laser-wakefield-accelerated electron beams (e beams) based on a controllable shock-induced density down-ramp injection that is achieved with an inserted tunable shock wave. The e beams are tunable from 400 to 800 MeV with charge ranges from 5 to 180 pC. e beams with high reproducibility (of ~95% in consecutive 100 shots) were produced in elaborate experiments with an average root- mean-square energy spread of 0.9% and an average divergence of 0.3 mrad. Three-dimensional particle-in-cell (PIC) simulations were also performed to accordingly verify and uncover the process of the injection and the acceleration. These tunable e beams will facilitate practical applications for advanced accelerator beam sources.