bluuebunny/crossref_metadata_2025 huggingface dataset:
Inter-Subunit Dynamics Controls Tunnel Formation During the Oxygenation Process in Hemocyanin Hexamers
Authors: Bux Khair , Shen Xiayu , Tariq Muhammad , Yin Junqi , Moin Syed Tarique , Bhowmik Debsindhu , Haider Shozeb
Published: September 2021

Hemocyanin from horseshoe crab in its active form is a homo-hexameric protein. It exists in open and closed conformations when transitioning between deoxygenated and oxygenated states. Here, we present a detailed dynamic atomistic investigation of the oxygenated and deoxygenated states of the hexameric hemocyanin using explicit solvent molecular dynamics simulations. We focus on the variation in solvent cavities and the formation of tunnels in the two conformational states. By employing principal component analysis and CVAE-based deep learning, we are able to differentiate between the dynamics of the deoxy- and oxygenated states of hemocyanin. Finally, our results identify the deoxygenated open conformation, which adopts a stable, closed conformation after the oxygenation process.
The Role of Substrate Mediated Allostery in the Catalytic Competency of the Bacterial Oligosaccharyltransferase PglB
Authors: Morgan Brittany R. , Massi Francesca
Published: September 2021

The oligosaccharyltransferase of Campylobacter lari (PglB) catalyzes the glycosylation of asparagine in the consensus sequence N-X-S/T, where X is any residue except proline. Molecular dynamics simulations of PglB bound to two different substrates were used to characterize the differences in the structure and dynamics of the substrate-enzyme complexes that can explain the higher catalytic efficiency observed for substrates containing threonine at the +2 position rather than serine. We observed that a threonine-containing substrate is more tightly bound than a serine-containing substrate. Because serine lacks a methyl group relative to threonine, the serine-containing peptide cannot stably form simultaneous van der Waals interactions with T316 and I572 as the threonine-containing substrate can. As a result, the peptide-PglB interaction is destabilized and the allosteric communication between the periplasmic domain and external loop EL5 is disrupted. These changes ultimately lead to the reorientation of the periplasmic domain relative to the transmembrane domain such that the two domains are further apart compared to PglB bound to the threonine-containing peptide. The crystal structure of PglB bound to the peptide and a lipid-linked oligosaccharide analog shows a pronounced closing of the periplasmic domain over the transmembrane domain in comparison to structures of PglB with peptide only, indicating that a closed conformation of the domains is needed for catalysis. The results of our studies suggest that lower enzymatic activity observed for serine versus threonine results from a combination of less stable binding and structural changes in PglB that influence the ability to form a catalytically competent state. This study illustrates a mechanism for substrate specificity via modulation of dynamic allosteric pathways.
Electronic Couplings and Electrostatic Interactions Behind the Light Absorption of Retinal Proteins
Authors: Fujimoto Kazuhiro J.
Published: September 2021

The photo-functional chromophore retinal exhibits a wide variety of optical absorption properties depending on its intermolecular interactions with surrounding proteins and other chromophores. By utilizing these properties, microbial and animal rhodopsins express biological functions such as ion-transport and signal transduction. In this review, we present the molecular mechanisms underlying light absorption in rhodopsins, as revealed by quantum chemical calculations. Here, symmetry-adapted cluster-configuration interaction (SAC-CI), combined quantum mechanical and molecular mechanical (QM/MM), and transition-density-fragment interaction (TDFI) methods are used to describe the electronic structure of the retinal, the surrounding protein environment, and the electronic coupling between chromophores, respectively. These computational approaches provide successful reproductions of experimentally observed absorption and circular dichroism (CD) spectra, as well as insights into the mechanisms of unique optical properties in terms of chromophore-protein electrostatic interactions and chromophore-chromophore electronic couplings. On the basis of the molecular mechanisms revealed in these studies, we also discuss strategies for artificial design of the optical absorption properties of rhodopsins.
Socializing Sensorimotor Contingencies
Authors: Lübbert Annika , Göschl Florian , Krause Hanna , Schneider Till R. , Maye Alexander , Engel Andreas K.
Published: September 2021

The aim of this review is to highlight the idea of grounding social cognition in sensorimotor interactions shared across agents. We discuss an action-oriented account that emerges from a broader interpretation of the concept of sensorimotor contingencies. We suggest that dynamic informational and sensorimotor coupling across agents can mediate the deployment of action-effect contingencies in social contexts. We propose this concept ofsocializing sensorimotor contingencies(socSMCs) as a shared framework of analysis for processes within and across brains and bodies, and their physical and social environments. In doing so, we integrate insights from different fields, including neuroscience, psychology, and research on human–robot interaction. We review studies on dynamic embodied interaction and highlight empirical findings that suggest an important role of sensorimotor and informational entrainment in social contexts. Furthermore, we discuss links to closely related concepts, such as enactivism, models of coordination dynamics and others, and clarify differences to approaches that focus on mentalizing and high-level cognitive representations. Moreover, we consider conceptual implications of rethinking cognition as social sensorimotor coupling. The insight that social cognitive phenomena like joint attention, mutual trust or empathy rely heavily on the informational and sensorimotor coupling between agents may provide novel remedies for people with disturbed social cognition and for situations of disturbed social interaction. Furthermore, our proposal has potential applications in the field of human–robot interaction where socSMCs principles might lead to more natural and intuitive interfaces for human users.
Ultrasound for Preoperatively Predicting Pathology Grade, Complete Cytoreduction Possibility, and Survival Outcomes of Pseudomyxoma Peritonei
Authors: Liang Lei , Han Xuedi , Zhou Nan , Xu Hongbin , Guo Jun , Zhang Qian
Published: September 2021

Objectives

This study aimed to investigate the value of using ultrasound (US) preoperatively for predicting pathological classification, complete cytoreduction possibility, and survival rate of patients with pseudomyxoma peritonei (PMP).

Methods

We retrospectively studied PMP patients who were scheduled for cytoreductive surgery between May 2009 and October 2019. US examination was performed before surgery. Factors related to high-grade pathology and poor completeness of cytoreduction (CC) score were identified. Associations between ultrasound characteristics and the survival status were also examined to identify independent predictive factors.

Results

PMP patients with clear ascites, abdominal lymph nodes, omental cake, abdominal mass, portal infiltration, and mesenteric involvement visible on US were considered to have high-grade pathology. Various US features were shown to be independent prognostic markers for inadequate cytoreduction in PMP patients. Portal infiltration and mesenteric involvement were significant prognostic factors for lower survival rates (hazard ratio = 3.092, 3.932, respectively). A visual nomogram including these factors was constructed to predict survival rates. The consistency index was 0.777, which reflected relatively high accuracy.

Conclusions

Preoperative US has the potential to predict pathological grade and resectability of PMP. Portal infiltration and mesenteric involvement were independent predictors of poor clinical outcomes in PMP patients. Furthermore, a simple-to-use nomogram derived from our study data may be a helpful visual tool in clinical practice to predict 1-, 2-, and 3-year survival rates for PMP patients.
Case Report: A Case of Infant Bronchial Dieulafoy's Disease and Article Review
Authors: Chen Yang , Mao Yiting , Cheng Xingfeng , Xiong Ruihua , Lan Ying , Chen Feng , Zhang Furong , Liu Jun , Wu Yanting
Published: September 2021

Background: Bronchial Dieulafoy's disease (BDD), characterized by constant diameter arterial malformation, is rare, especially among infants. The pathogenesis and clinical features of pediatric patients are unknown. Misdiagnosis and biopsy operations may lead to potential massive hemorrhage, which endangers the patient's life.

Case Presentation: Here, we present a case of a 9-month-old boy who was diagnosed with BDD with massive hemoptysis. The boy was cured by embolization of the bronchial artery and was in good health at the 1-year follow-up. In addition, we searched PubMed, Google Scholar, and Web of Science databases using keyword “Bronchial Dieulafoy's Disease (BDD)” and found six additional cases of pediatric BDD.

Conclusion: It is still insufficient to draw a conclusion about the origin of the disease. Bronchial angiography and endobronchial ultrasonography are considered promising methods to diagnose Dieulafoy's disease of the bronchus. Bronchoscopy with transbronchial biopsy should not be deployed due to the high risk of fatal hemorrhage. Explicit clinical case reports of BDD are needed to enhance the understanding of this rare disease.
Chromosomal Microarray in Children With Developmental Delay: The Experience of a Tertiary Center in Korea
Authors: Yang Eun Hye , Shin Yong Beom , Choi Soo Han , Yoo Hye Won , Kim Hye Young , Kwak Min Jung , Park Kyung Hee , Bae Mi Hye , Kong Ju Hyun , Lee Yun-Jin , Nam Sang Ook , Kim Young Mi
Published: September 2021

Background and Objectives: Chromosomal microarray (CMA) is a first-tier genetic test for children with developmental delay (DD), intellectual disability (ID), autism spectrum disorders (ASDs), and multiple congenital anomalies (MCA). In this study, we report our experiences with the use of CMA in Korean children with unexplained DD/ID.

Methods: We performed CMA in a cohort of 308 children with DD/ID between January 2010 and September 2020. We also retrospectively reviewed their medical records. The Affymetrix CytoScan 750 K array with an average resolution of 100 kb was used to perform CMA.

Results: Comorbid neurodevelopmental disorders were ASD (37 patients; 12.0%), epilepsy (34 patients; 11.0%), and attention deficit hyperactivity disorders (12 patients; 3.9%). The diagnostic yield was 18.5%. Among the 221 copy number variants (CNVs) identified, 70 CNVs (57 patients; 18.5%) were pathogenic. Deletion CNVs were more common among pathogenic CNVs (PCNVs) than in non-PCNVs (P < 0.001). The size difference between PCNVs and non-PCNVs was not significant (P = 0.023). The number of included genes within CNV intervals was significantly higher in PCNVs (average 8.6; 0–347) than in non-PCNVs (average 47.5; 1–386) (P < 0.001). Short stature and hearing difficulty were also more common in the PCNV group than in the non-PCNV group (P = 0.010 and 0.070, respectively).

Conclusion: This study provides additional evidence for the usefulness of CMA in genetic testing of children with DD/ID in Korea. The pathogenicity of CNVs correlated with the number of included genes within the CNV interval and deletion type of the CNVs, but not with CNV size.
Diminishment of Nrf2 Antioxidative Defense Aggravates Nephrotoxicity of Melamine and Oxalate Coexposure
Authors: Wu Chia-Fang , Liu Chia-Chu , Tsai Yi-Chun , Chen Chu-Chih , Wu Ming-Tsang , Hsieh Tusty-Jiuan
Published: September 2021

Chronic kidney disease (CKD) usually causes devastating healthy impacts on patients. However, the causes affecting the decline of kidney function are not fully revealed, especially the involvement of environmental pollutants. We have revealed that exposure to melamine, a ubiquitous chemical in daily life, is linked to adverse kidney outcomes. Hyperoxaluria that results from exposure to excessive oxalate, a potentially nephrotoxic terminal metabolite, is reportedly associated with CKD. Thus, we explored whether interaction of these two potential nephrotoxicants could enhance kidney injury. We established a renal proximal tubular HK-2 cell model and a Sprague–Dawley rat model of coexposure to melamine with sodium oxalate or hydroxy-L-proline to investigate the interacting adverse effects on kidneys. Melamine and oxalate coexposure enhanced the levels of reactive oxygen species, lipid peroxidation and oxidative DNA damage in the HK-2 cells and kidney tissues. The degrees of tubular cell apoptosis, tubular atrophy, and interstitial fibrosis were elevated under the coexposed condition, which may result from the diminishment of Nrf2 antioxidative capacity. To conclude, melamine and oxalate coexposure aggravates renal tubular injury via impairment of antioxidants. Accumulative harmful effects of exposure to multiple environmental nephrotoxicants should be carefully investigated in the etiology of CKD progression.
Synergy of the Inhibitory Action of Polyphenols Plus Vitamin C on Amyloid Fibril Formation: Case Study of Human Stefin B
Authors: Jahić Mujkić Alma , Tušek Žnidarič Magda , Berbić Selma , Žerovnik Eva
Published: September 2021

In order to study how polyphenols and vitamin C (vitC) together affect protein aggregation to amyloid fibrils, we performed similar in vitro studies as before using stefin B as a model and a potentially amyloid-forming protein (it aggregates upon overexpression, under stressful conditions and some progressive myoclonus epilepsy of tape 1—EPM1-missense mutations). In addition to the chosen polyphenol, this time, we added a proven antioxidant concentration of 0.5 mM vitC into the fibrillation mixture and varied concentrations of resveratrol, quercetin, and curcumin. Synergy with vitC was observed with curcumin and quercetin.
Microbial Biosurfactant: A New Frontier for Sustainable Agriculture and Pharmaceutical Industries
Authors: Kumar Ajay , Singh Sandeep Kumar , Kant Chandra , Verma Hariom , Kumar Dharmendra , Singh Prem Pratap , Modi Arpan , Droby Samir , Kesawat Mahipal Singh , Alavilli Hemasundar , Bhatia Shashi Kant , Saratale Ganesh Dattatraya , Saratale Rijuta Ganesh , Chung Sang-Min , Kumar Manu
Published: September 2021

In the current scenario of changing climatic conditions and the rising global population, there is an urgent need to explore novel, efficient, and economical natural products for the benefit of humankind. Biosurfactants are one of the latest explored microbial synthesized biomolecules that have been used in numerous fields, including agriculture, pharmaceuticals, cosmetics, food processing, and environment-cleaning industries, as a source of raw materials, for the lubrication, wetting, foaming, emulsions formulations, and as stabilizing dispersions. The amphiphilic nature of biosurfactants have shown to be a great advantage, distributing themselves into two immiscible surfaces by reducing the interfacial surface tension and increasing the solubility of hydrophobic compounds. Furthermore, their eco-friendly nature, low or even no toxic nature, durability at higher temperatures, and ability to withstand a wide range of pH fluctuations make microbial surfactants preferable compared to their chemical counterparts. Additionally, biosurfactants can obviate the oxidation flow by eliciting antioxidant properties, antimicrobial and anticancer activities, and drug delivery systems, further broadening their applicability in the food and pharmaceutical industries. Nowadays, biosurfactants have been broadly utilized to improve the soil quality by improving the concentration of trace elements and have either been mixed with pesticides or applied singly on the plant surfaces for plant disease management. In the present review, we summarize the latest research on microbial synthesized biosurfactant compounds, the limiting factors of biosurfactant production, their application in improving soil quality and plant disease management, and their use as antioxidant or antimicrobial compounds in the pharmaceutical industries.
Impact of Heavy Metals on Human Male Fertility—An Overview
Authors: López-Botella Andrea , Velasco Irene , Acién Maribel , Sáez-Espinosa Paula , Todolí-Torró José-Luis , Sánchez-Romero Raquel , Gómez-Torres María José
Published: September 2021

Heavy metals are endocrine disruptors which interfere with processes mediated by endogenous hormones of the organism, negatively affecting endocrine functions. Some studies have correlated heavy metal exposure with male infertility. However, the number of studies conducted on humans are limited. Therefore, the aim of this study is to summarize the current knowledge on how heavy metals influence human male fertility. Hence, three distinct databases were consulted—PubMed, Scopus and Web of Science—using single keywords and combinations of them. The total number of identified articles was 636. Nevertheless, by using the inclusion and exclusion criteria, 144 articles were finally included in this work. Results display that the development of adequate instruments for heavy metal assessment may play an important function in human male fertility diagnosis and treatment. Furthermore, clinical trials could be useful to confirm the role of heavy metals in human male fertility diagnosis. Overall, further research is required to fully understand the molecular and cellular basis of the influence of environmental and occupational exposure to heavy metals on human male infertility and reproductive outcomes.
Characterization of Two Variants at Met 1 of the Human LDLR Gene Encoding the Same Amino Acid but Causing Different Functional Phenotypes
Authors: Graça Rafael , Fernandes Rafael , Alves Ana Catarina , Menezes Juliane , Romão Luísa , Bourbon Mafalda
Published: September 2021

Familial hypercholesterolemia (FH) is the most common genetic disorder of lipid metabolism, characterized by increased levels of total and LDL plasma cholesterol, which leads to premature atherosclerosis and coronary heart disease. FH phenotype has considerable genetic heterogeneity and phenotypic variability, depending on LDL receptor activity and lifestyle. To improve diagnosis and patient management, here, we characterized two single nucleotide missense substitutions at Methionine 1 of the human LDLR gene (c.1A>T/p.(Met1Leu) and c.1A>C/p.(Met1Leu)). We used a combination of Western blot, flow cytometry, and luciferase assays to determine the effects of both variants on the expression, activity, and synthesis of LDLR. Our data show that both variants can mediate translation initiation, although the expression of variant c.1A>T is very low. Both variants are in the translation initiation codon and codify for the same amino acid p.(Met1Leu), yet they lead to different levels of impairment on LDLR expression and activity, corroborating different efficiencies of the translation initiation at these non-canonical initiation codons. The functional data of these variants allowed for an improved American College of Medical Genetics (ACMG) classification for both variants, which can allow a more personalized choice of the lipid-lowering treatment and dyslipidemia management, ultimately improving patients’ prognosis.
Can Propolis Be a Useful Adjuvant in Brain and Neurological Disorders and Injuries? A Systematic Scoping Review of the Latest Experimental Evidence
Authors: Zulhendri Felix , Perera Conrad O. , Tandean Steven
Published: September 2021

Propolis has been used therapeutically for centuries. In recent years, research has demonstrated its efficacy as a potential raw material for pharmaceuticals and nutraceuticals. The aim of the present scoping review is to examine the latest experimental evidence regarding the potential use of propolis in protecting the brain and treating neurological disorders and injuries. A systematic scoping review methodology was implemented. Identification of the research themes and knowledge gap was performed. After applying the exclusion criteria, a total of 66 research publications were identified and retrieved from Scopus, Web of Science, Pubmed, and Google Scholar. Several key themes where propolis is potentially useful were subsequently identified, namely detoxification, neuroinflammation, ischemia/ischemia-reperfusion injury/traumatic brain injury, Alzheimer’s disease, Parkinson’s disease, and epilepsy models, depression, cytotoxicity, cognitive improvement, regenerative medicine, brain infection, and adverse effects. In conclusion, propolis is shown to have protective and therapeutic benefits in alleviating symptoms of brain and neurological disorders and injuries, demonstrated by various in vitro studies, animal models, and human clinical trials. Further clinical research into this area is needed.
Cisd2 Preserves the Youthful Pattern of the Liver Proteome during Natural Aging of Mice
Authors: Huang Chen-Hua , Huang Yi-Long , Shen Zhao-Qing , Lin Chao-Hsiung , Tsai Ting-Fen
Published: September 2021

Cisd2 (CDGSH iron sulfur domain 2) is a pro-longevity gene that extends the lifespan and health span of mice, ameliorates age-associated structural damage and limits functional decline in multiple tissues. Non-alcoholic fatty liver disease (NAFLD), which plays an important role in age-related liver disorders, is the most common liver disease worldwide. However, no medicines that can be used to specifically and effectively treat NAFLD are currently approved for this disease. Our aim was to provide pathological and molecular evidence to show that Cisd2 protects the liver from age-related dysregulation of lipid metabolism and protein homeostasis. This study makes four major discoveries. Firstly, a persistently high level of Cisd2 protects the liver from age-related fat accumulation. Secondly, proteomics analysis revealed that Cisd2 ameliorates age-related dysregulation of lipid metabolism, including lipid biosynthesis and β-oxidation, in mitochondria and peroxisomes. Thirdly, Cisd2 attenuates aging-associated oxidative modifications of proteins. Finally, Cisd2 regulates intracellular protein homeostasis by maintaining the functionality of molecular chaperones and protein synthesis machinery. Our proteomics findings highlight Cisd2 as a novel molecular target for the development of therapies targeting fatty liver diseases, and these new therapies are likely to help prevent subsequent malignant progression to cirrhosis and hepatocellular carcinoma.
Predictions about the Cognitive Consequences of Language Switching on Executive Functioning Inspired by the Adaptive Control Hypothesis Fail More Often than Not
Authors: Paap Kenneth R. , Mason Lauren , Anders-Jefferson Regina
Published: September 2021

The adaptive control hypothesis developed by Green and Abutalebi is the most influential theory of bilingual language control. The focus of this article is on the predictions that other researchers have derived based on the three different modes of interactional context described by the hypothesis. Foremost, that dual-language contexts should enhance domain-general executive functions more than single-language contexts. Several recent and ambitious behavioral tests of these predictions are reviewed. Although there was some evidence that dual-language contexts are associated with smaller switch costs, the evidence is inconsistent and there were no similar advantages for inhibitory control. The hypothesis also predicts neuroanatomical adaptations to the three types of interactional context. A careful evaluation of the relevant fMRI and ERP studies that take into account whether behavioral differences align with neuroscience differences and resolves valence ambiguities led to the conclusion that the neuroscience evidence for the hypothesis is, at best, inconsistent. The study also includes new analyses of two large-sample studies that enable the identification of relatively pure cases of single-language bilinguals, dual-language bilinguals, and dense-code switchers. Across nine different measures of executive functioning, the predicted advantage of the dual-language context never materialized. The hypotheses derived from the adaptive control hypothesis do not accurately predict behavioral performance on tests of executive functioning and do not advance our understanding as to what dimensions of bilingualism may lead to enhancements in specific components of executive functioning.
Infants’ Sleep: Israeli Parents’ Knowledge, Attitudes and Practices
Authors: Shatz Anat , Joseph Leon , Korn Liat
Published: September 2021

The purpose of the study was to assess Israeli parents’ knowledge of and attitudes towards practices promoting infants’ safe sleep and their compliance with such practices. Researchers visited the homes of 335 parents in 59 different residential locations in Israel and collected their responses to structured questionnaires. SPSS 25 statistical package for data analysis was used. Attitude scales were created after the reliability tests and scaled means of parental attitudes were compared between independent groups differentiated by gender, ethnicity, and parental experience. A logistic regression was run to predict the outcome variable of babies’ sleep positions. The total knowledge score was significantly higher for women (56.3%) than for men (28.6%; p < 0.001). Arabs were more committed to following recommendations (29.3%) than Jews (26.9%; p < 0.001). Consistent with safe sleep recommendations, 92% of the sampled parents reported avoiding bedsharing and 89% reported using a firm mattress and fitted sheets. The risk of not placing a baby to sleep in a supine position was higher among older parents (adjusted odds ratio—AOR = 0.36, 95%CI 0.16–0.82), smoking fathers (AOR = 2.66, 95%CI 1.12–6.33), parents who did not trust recommendations (AOR = 4.03, 95%CI 1.84–8.84), parents not committed to following recommendations (AOR = 2.83, 95%CI 1.21–6.60), and parents whose baby slept in their room (AOR = 0.38, 95%CI 0.17–0.88). Knowledge of safe sleep recommendations was not associated with actual parental practices. Trust of and commitment to recommendations were positively correlated with safe sleep position practices. It is essential to develop ethnic-/gender-focused intervention programs.
Revealing the Correlation between Altered Skin Lipids Composition and Skin Disorders
Authors: Drakou Katerina , Tsianni Andrea , Vrani Faye , Kefala Valia , Rallis Efstathios
Published: September 2021

Human skin layers serve as a barrier between the body and the environment, by preventing water loss and blocking the entry of chemicals, allergens, and microbes. Recent data showed that skin lipids are vital ‘key players’ of several functions and mechanisms performing in the skin, such as, barrier function and microbiome composition. Abnormalities in lipid composition have been observed in inflammatory cutaneous diseases with a disrupted skin barrier. This review aims to demonstrate the fundamental role of keratinocytes, sebocytes, and microbiome-derived lipids in the maintenance of the skin barrier. Furthermore, it would reveal the correlation between altered skin lipids’ composition, microbiome, and the occurrence of certain dermatological disorders such as acne vulgaris, atopic dermatitis, psoriasis, and rosacea.
Performance Evaluation of Forecasting Strategies for Electricity Consumption in Buildings
Authors: Hadri Sarah , Najib Mehdi , Bakhouya Mohamed , Fakhri Youssef , El Arroussi Mohamed
Published: September 2021

In this paper, three main approaches (univariate, multivariate and multistep) for electricity consumption forecasting have been investigated. In fact, three major algorithms (XGBOOST, LSTM and SARIMA) have been evaluated in each approach with the main aim to figure out which one performs the best in forecasting electricity consumption. The motivation behind this work is to assess the forecasting accuracy and the computational time/complexity for an embedded forecasting and model training at the smart meter level. Moreover, we investigate the deployment of the most efficient model in our platform for an online electricity consumption forecasting. This solution will serve for deploying predictive control solutions for efficient energy management in buildings. As a proof of concept, an already existing public dataset has been used. These data were mainly collected thanks to the usage of already deployed sensors. These provide accurate data related to occupancy (e.g., presence) as well as contextual data (e.g., disaggregated electricity consumption of equipment). Experiments have been conducted and the results showed the effectiveness of these algorithms, used in each approach, for short-term electricity consumption forecasting. This has been proved by performance evaluation and error calculations. The obtained results mainly shed light on the challenging trade-off between embedded forecasting model training and processing for being deployed in smart meters for electricity consumption forecasting.
Citrobacter braakii Yield False-Positive Identification as Salmonella, a Note of Caution
Authors: Pławińska-Czarnak Joanna , Wódz Karolina , Kizerwetter-Świda Magdalena , Nowak Tomasz , Bogdan Janusz , Kwieciński Piotr , Kwieciński Adam , Anusz Krzysztof
Published: September 2021

Background: Globally, Salmonella enterica is one of the leading causes of foodborne illness in humans. Food of animal origin is obligatorily tested for the presence of this pathogen. Unfortunately, in meat and meat products, this is often hampered by the presence of background microbiota, which may present as false-positive Salmonella. Methods: For the identification of Salmonella spp. from meat samples of beef, pork, and poultry, the authorized detection method is PN-EN ISO 6579-1:2017-04 with the White–Kauffmann–Le Minor scheme, two biochemical tests: API 20E and VITEK II, and a real-time PCR-based technique. Results: Out of 42 presumptive strains of Salmonella, 83.3% Salmonella enterica spp. enterica, 14.3% Citrobacter braakii, and 12.4% Proteus mirabilis were detected from 180 meat samples. Conclusions: Presumptive strains of Salmonella should be identified based on genotypic properties such as DNA-based methods. The aim of this study was the isolation and identification of Salmonella spp. from miscellaneous meat sorts: beef, pork, and poultry.
Microstructural Analysis of Whey/Soy Protein Isolate Mixed Gels Using Confocal Raman Microscopy
Authors: Gómez-Mascaraque Laura G. , Pinho Samantha C.
Published: September 2021

This work explores the potential of confocal Raman microscopy to investigate the microstructure of mixed protein gel systems. Heat-set protein gels were prepared using whey protein isolate (WPI), soy protein isolate (SPI), and mixtures thereof, with a total of five different whey-to-soy protein ratios (100, 75, 50, 25, and 0%). These were analysed using confocal Raman microscopy, and different data analysis approaches were used to maximize the amount of structural and compositional information extracted from the spectral datasets generated, including both univariate and multivariate analysis methods. Small spectral differences were found between pure WPI and SPI gels, mainly attributed to conformational differences (amide bands), but SPI exhibited considerably greater auto-fluorescence than WPI. The univariate analysis method allowed for a rapid microstructural analysis, successfully mapping the distribution of protein and water in the gels. The greater fluorescence of the capsule-like structures found in the mixed gels, compared to other regions rich in proteins, suggested that these may be enriched in soy proteins. Further analysis, using a multivariate approach, allowed us to distinguish proteins with different levels of hydration within the gels and to detect non-proteinaceous compounds. Raman microscopy proved to be particularly useful to detect the presence of residual lipids in protein gels.
A Novel Exercise Facilitation Method in Combination with Cognitive Behavioral Therapy Using the Ikiiki Rehabilitation Notebook for Intractable Chronic Pain: Technical Report and 22 Cases
Authors: Kimura Shinji , Hosoi Masako , Otsuru Naofumi , Iwasaki Madoka , Matsubara Takako , Mizuno Yasuyuki , Nishihara Makoto , Murakami Takanori , Yamazaki Ryo , Ijiro Hajime , Anno Kozo , Watanabe Kei , Kitamura Takuya , Yamada Shouhei
Published: September 2021

Recent clinical practice guidelines for chronic pain indicate, with a high evidence level, that the combination of exercise and cognitive behavioral therapy (CBT) is effective. The purpose of this study was to evaluate the effectiveness of an exercise facilitation method in combination with CBT using the “Ikiiki Rehabilitation Notebook” for patients with intractable chronic pain. “Ikiiki” means active in Japanese. A total of 22 cases with chronic low back (n = 13), lower extremity (n = 8), or neck (n = 1) pain were treated using this notebook. Two cases dropped out, leaving 22 cases. Each case was evaluated in terms of the numerical rating scale (NRS) of the pain, activities of daily living (ADL), pain catastrophizing scale (PCS), and quality of life (QOL) at pretreatment and post-treatment. The endpoint of the method was to achieve the long-term goals set by the patients. The mean treatment period was 11.2 months. The outcomes were as follows: improvement of presenteeism: nine cases; enhanced participation in hobbies: seven cases; improved school attendance: two cases; return to work: one case; improvement of self-care and/or self-efficacy: three cases. The NRS, ADL, PCS, and QOL were significantly improved after the treatment. This method is possibly valuable for educating patients about the cause and treatment of chronic pain and actively facilitating exercise and social participation. Further studies are needed to investigate the effectiveness of using this notebook for the patient with intractable chronic pain.
Link between Excessive Smartphone Use and Sleeping Disorders and Depression among South Korean University Students
Authors: Islam Maidul
Published: September 2021

The purpose of this study was to explore the link between smartphone use and sleeping disorders and depression among university students in South Korea. South Korea has the highest mobile phone penetration rate as well as the highest rate of suicide of any of the Organization for Economic Cooperation and Development (OECD) nations, thus making this study of great importance. The core aim was to see whether the excessive use of smartphones has an association with sleeping disorders and depression. A cross-sectional analysis was performed to establish if there was any link between smartphone use and sleeping disorders and depression. Samples from 188 participants were used for this study. Data were collected using two well-established questionnaires, the Center for Epidemiologic Studies-Depression (CES-D) and the Athene Insomnia Scale (AIS), as well as a few questions on smartphone use. A few demographic questions were added to the questionnaire. The results of this study concluded that a significant relationship exists between smartphone use and depression. However, the finding of this research could not uncover a significant relationship between smartphone use and sleeping disorders among university students in South Korea. The excessive use of smartphones shows a relationship to an unhealthy lifestyle. There is a clear indication that the overuse of smartphones could be linked to depression. Furthermore, the study found that students with depression also tend to have sleeping disorders.
Effectiveness of a Mindfulness-Based Intervention Program for Women Family Caregivers of Older Adults
Authors: Fernández-Portero Cristina , Alarcón David , Gallardo-Flores Ana , Amián Josue G. , Sánchez-Medina Jose A.
Published: September 2021

Background: The objective of this study was to analyze the effectiveness of a mindfulness-based intervention program for the promotion of well-being and health in family caregivers. Methods: The participants were 111 family women caregivers aged between 33 and 75 years old. This was a double-blinded randomized controlled trial. The mindfulness intervention program lasted 12 weeks. The experimental group underwent mindfulness and acceptance-based interventions (MABIs). The control group performed physical activity training. Results: A cross-lagged panel analysis was computed with the two waves of health, well-being, burden and resilience and age in years and intervention as predictors. The cross-lagged path model fit well χ2 (8) = 7.179, p = 0.51, root mean square error of approximation (RMSEA) = 0.00, comparative fit index (CFI) = 1.00, standardized root mean square residual (SRMR) = 0.05. The mindfulness intervention program was a significant predictor accounting for decreasing health problems (β = −0.292, p < 0.01) and burden (β = −0.190, p < 0.01) and increasing well-being (β = 0.107, p < 0.05) at post-test. Conclusions: Mindfulness-based intervention programs are effective in coping with the burden of family caregivers and, in turn, in promoting resilience, well-being and health among caregivers. Our findings encourage clinical uses of mindfulness interventions to promote health.
The Intriguing Relationships of von Willebrand Factor, ADAMTS13 and Cardiac Disease
Authors: Reardon Benjamin , Pasalic Leonardo , Favaloro Emmanuel J.
Published: September 2021

von Willebrand factor (VWF) is an adhesive protein involved in primary hemostasis and facilitates platelet adhesion to sites of vascular injury, thereby promoting thrombus formation. VWF exists in plasma as multimers of increasing size, with the largest (high molecular weight; HMW) expressing the greatest functional activity. A deficiency of VWF is associated with a bleeding disorder called von Willebrand disease (VWD), whereas an excess of VWF, in particular the HMW forms, is associated with thrombosis. ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type 1 motif-13), also known as VWF-cleaving protease, functions to moderate the activity of VWF by cleaving multimers of VWF and limiting the expression of the largest multimers of VWF. A deficiency of ADAMTS13 is therefore associated with an excess of (HMW forms of) VWF, and thus thrombosis. Indeed, any disturbance of the VWF/ADAMTS13 ratio or ‘axis’ may be associated with pathophysiological processes, including prothrombotic tendency. However, both thrombosis or bleeding may be associated with such disturbances, depending on the presenting events. This review evaluates the relationship of VWF and ADAMTS13 with cardiac disease, including cardiac failure, and associated pathophysiology.
Application of Basis Functions for Hull Form Surface Modification
Authors: Feng Baiwei , Zhan Chengsheng , Liu Zuyuan , Cheng Xide , Chang Haichao
Published: September 2021

Basis functions are key in constructing interpolation equations in hull surface modification based on radial basis functions (RBF) interpolation. However, few have studied the selection of basis functions in depth. By comparing several typical basis functions through a theoretical analysis and two-dimensional modification examples, the Wendland ψ3,1 (W) function is selected. The advantages of hull form surface modification based on W function interpolation are further validated through a case study. Finally, the modification method is used to optimize a trimaran model. An optimal hull form with fair lines is obtained, and its wave-making resistance coefficient and total resistance are reduced by 8.3% and 3.8%, respectively, compared to those of the original model. These findings not only further illustrate that the W function is relatively suitable for hull form surface modification, but also validate the feasibility and value of the RBF interpolation-based surface modification method in engineering practice.
Sound Propagation with Undulating Bottom in Shallow Water
Authors: Liu Dai , Li Zhenglin , Wang Guangxu , Liu Yunfeng
Published: September 2021

An undulating bottom in shallow water has a significant effect on sound propagation. An acoustic propagation experiment was carried out in the East China Sea in 2020. Measurements along two separate propagation tracks with flat and undulating bottoms were obtained. Abnormal transmission losses (TLs) were observed along the track with the undulating bottom. By using the parabolic equation model RAM and ray theory, these abnormal TLs and the distribution of the sound field energy were analyzed. Numerical simulations indicate that under the shallow water condition with a negative thermocline and for a high frequency (1000 Hz), the incidence and reflection angles of sound rays on the sea bottom are changed due to the undulating sea bottom. The larger the inclination angle of the undulating bottom, the greater the grazing angle changes. These angles changes lead to different sound propagation paths for the undulating bottom and the flat bottom, resulting in the difference of TLs at a certain distance and depth. The undulating bottom will cause energy convergence in the mixed layer when the source and receiver locate above the thermocline.
Antifungal Activity of the Phenolic Compounds Ellagic Acid (EA) and Caffeic Acid Phenethyl Ester (CAPE) against Drug-Resistant Candida auris
Authors: Possamai Rossatto Fernanda Cristina , Tharmalingam Nagendran , Escobar Iliana E. , d’Azevedo Pedro Alves , Zimmer Karine Rigon , Mylonakis Eleftherios
Published: September 2021

Candida auris is an emerging healthcare-associated fungal pathogen that has become a serious global health threat. Current treatment options are limited due to drug resistance. New therapeutic strategies are required to target this organism and its pathogenicity. Plant polyphenols are structurally diverse compounds that present a vast range of biological properties. In the present study, plant-derived molecules ellagic acid (EA) and caffeic acid phenethyl ester (CAPE) were investigated for their antifungal and antivirulence activities against Candida auris. We also tested against C. albicans. The minimum inhibitory concentration (MIC) for EA ranged from 0.125 to 0.25 µg/mL and for CAPE ranged from 1 to 64 µg/mL against drug-resistant C. auris strains. Killing kinetics determined that after 4 h treatment with CAPE, there was a complete reduction of viable C. auris cells compared to fluconazole. Both compounds might act by modifying the fungal cell wall. CAPE significantly reduced the biomass and the metabolic activity of C. auris biofilm and impaired C. auris adhesion to cultured human epithelial cells. Furthermore, both compounds prolonged the survival rate of Galleria mellonella infected by C. auris (p = 0.0088 for EA at 32 mg/kg and p = 0.0028 for CAPE at 4 mg/kg). In addition, EA at 4 μg/mL prolonged the survival of C. albicans-infected Caenorhabditis elegans (p < 0.0001). CAPE was not able to prolong the survival of C. albicans-infected C. elegans. These findings highlight the antifungal and antivirulence effects of EA and CAPE against C. auris, and warrant further investigation as novel antifungal agents against drug-resistant infections.
Price Transmission in Cotton Futures Market: Evidence from Three Countries
Authors: Singh Amrinder , Soni Tarun Kumar
Published: September 2021

This study examines the price transmission between cotton prices in U.S., Indian, and Chinese futures markets. We focus on studying the long-run price movements using cointegration and alternate causality tests. The empirical results indicate the following: (a) the U.S. cotton futures market continues to be the most dominant market, and it leads price changes in India and China; (b) the cotton prices in India also impacts the cotton prices in China as we report a unidirectional relationship flowing from India to China; (c) there is duality of direction of price transmission for U.S. and Chinese commodity markets as we document bi-directional causality between U.S. to Chinese cotton futures for the entire period and uni-directional causality from U.S. to Chinese markets for the two sub-periods; (d) the long-term relationship between the three markets has seen a significant shift as documented by the absence of cointegration which may be due to changes in government policy, especially in India and China specifically after 2014. Overall, results provide support for further reforms especially for Indian and Chinese commodity exchanges so that they can play a vital role in the price discovery process especially for commodities that are largely produced or consumed in these economies.
Market Behavior in the Face of Political Violence: Evidence from Tsarist Russia
Authors: Hartwell Christopher A.
Published: September 2021

Even efficient financial markets may break down under periods of prolonged stress, especially when the ramification of an event is unclear. Political violence is such an event, sending immediate signals about possible impact on firm valuations but unclear information about the future viability of existing institutions. This paper examines the effect of political violence in 19th century Russia on its stock market; using a battery of unit root and variance ratio tests, the evidence is that Russian financial markets were mostly efficient in processing short-term information from political violence. However, when violence was at its peak between the assassination of the Tsar in 1881 and the 1905 revolution, large deviations from efficiency can be detected, as markets were unsure about the viability of the existing rules of the game.
Asymptotics of the Sum of a Sine Series with a Convex Slowly Varying Sequence of Coefficients
Authors: Solodov Aleksei
Published: September 2021

We study the asymptotic behavior in a neighborhood of zero of the sum of a sine series g(b,x)=∑k=1∞bksinkx whose coefficients constitute a convex slowly varying sequence b. The main term of the asymptotics of the sum of such a series was obtained by Aljančić, Bojanić, and Tomić. To estimate the deviation of g(b,x) from the main term of its asymptotics bm(x)/x, m(x)=[π/x], Telyakovskiĭ used the piecewise-continuous function σ(b,x)=x∑k=1m(x)−1k2(bk−bk+1). He showed that the difference g(b,x)−bm(x)/x in some neighborhood of zero admits a two-sided estimate in terms of the function σ(b,x) with absolute constants independent of b. Earlier, the author found the sharp values of these constants. In the present paper, the asymptotics of the function g(b,x) on the class of convex slowly varying sequences in the regular case is obtained.
Magic Square and Arrangement of Consecutive Integers That Avoids k-Term Arithmetic Progressions
Authors: Sim Kai An , Wong Kok Bin
Published: September 2021

In 1977, Davis et al. proposed a method to generate an arrangement of [n]={1,2,…,n} that avoids three-term monotone arithmetic progressions. Consequently, this arrangement avoids k-term monotone arithmetic progressions in [n] for k≥3. Hence, we are interested in finding an arrangement of [n] that avoids k-term monotone arithmetic progression, but allows k−1-term monotone arithmetic progression. In this paper, we propose a method to rearrange the rows of a magic square of order 2k−3 and show that this arrangement does not contain a k-term monotone arithmetic progression. Consequently, we show that there exists an arrangement of n consecutive integers such that it does not contain a k-term monotone arithmetic progression, but it contains a k−1-term monotone arithmetic progression.
On the Approximated Solution of a Special Type of Nonlinear Third-Order Matrix Ordinary Differential Problem
Authors: Defez Emilio , Ibáñez Javier , Alonso José M. , Tung Michael M. , Real-Herráiz Teresa
Published: September 2021

Matrix differential equations are at the heart of many science and engineering problems. In this paper, a procedure based on higher-order matrix splines is proposed to provide the approximated numerical solution of special nonlinear third-order matrix differential equations, having the form Y(3)(x)=f(x,Y(x)). Some numerical test problems are also included, whose solutions are computed by our method.