bluuebunny/crossref_metadata_2025 huggingface dataset:
Neural Sensitivity to Mutual Information in Intermediate-Complexity Face Features Changes during Childhood
Authors: Balas Benjamin , Harel Assaf , Auen Amanda , Saville Alyson
Published: June 2019

One way in which face recognition develops during infancy and childhood is with regard to the visual information that contributes most to recognition judgments. Adult face recognition depends on critical features spanning a hierarchy of complexity, including low-level, intermediate, and high-level visual information. To date, the development of adult-like information biases for face recognition has focused on low-level features, which are computationally well-defined but low in complexity, and high-level features, which are high in complexity, but not defined precisely. To complement this existing literature, we examined the development of children’s neural responses to intermediate-level face features characterized using mutual information. Specifically, we examined children’s and adults’ sensitivity to varying levels of category diagnosticity at the P100 and N170 components. We found that during middle childhood, sensitivity to mutual information shifts from early components to later ones, which may indicate a critical restructuring of face recognition mechanisms that takes place over several years. This approach provides a useful bridge between the study of low- and high-level visual features for face recognition and suggests many intriguing questions for further investigation.
Evidence of the Climate Mitigation Effect of Green Roofs—A 20-Year Weather Study on an Extensive Green Roof (EGR) in Northeast Germany
Authors: Köhler Manfred , Kaiser Daniel
Published: June 2019

Approximately 10 km2 of new green roofs are built in Germany every year. About 85% of these are Extensive Green Roofs (EGR). An EGR with several research features was installed on new buildings belonging to the University of Applied Sciences Neubrandenburg in 1999. The results of the almost 20-year permanent survey of the climate effects of the green roof in contrast to gravel roofs are presented here. High-quality sensors, similar to those used by official weather stations, are in use, and data is collected every 10 s and aggregated to hourly values which enable comparisons to official measurements made by the DWD in Neubrandenburg and Berlin. The results show the typical urban heat island effect (UHI) and the mitigation effect of EGR. Whilst the temperature increased over the years due to the urban heat island effect, the temperature within the growing media in the green roof remained constant. The EGR has a stabilization effect of 1.5 K. This is good news for all those seeking a UHI mitigation solution for city centers. In a best-case scenario, the green roof potential of cities in Germany is between 3 and 8%. A value of 50% can be achieved for all buildings; roofs represent about ¼ of urban surfaces, and the cooling effect of 1.5 K in 20 years is a reasonable contribution to cooling cities and achieving environmental goals by greening urban surfaces.
Diagnostic Leukapheresis Enables Reliable Transcriptomic Profiling of Single Circulating Tumor Cells to Characterize Inter-Cellular Heterogeneity in Terms of Endocrine Resistance
Authors: Reinhardt Florian , Franken André , Meier-Stiegen Franziska , Driemel Christiane , Stoecklein Nikolas H. , Fischer Johannes C. , Niederacher Dieter , Ruckhaeberle Eugen , Fehm Tanja , Neubauer Hans
Published: June 2019

Circulating tumor cells (CTCs) hold great promise with regard to prognosis, treatment optimization, and monitoring of breast cancer patients. Single CTC transcriptome profiling might help reveal valuable information concerning intra-patient heterogeneity relevant to therapeutic interventions. In this study, we combined Diagnostic Leukapheresis (DLA), which is a microfluidic enrichment using the ParsortixTM system, micromanipulation with CellCelectorTM and subsequent single cell multi-marker transcriptome profiling. First, a PCR panel consisting of 30 different endocrine resistance and phenotypic marker genes was validated for single cell profiling by using different breast cancer cell lines. Second, this panel was applied to characterize uncultured and cultured CTCs, which were enriched from a cryopreserved DLA product obtained from a patient suffering from metastatic breast cancer resistant to endocrine therapy. Gene expression profiles of both CTC populations uncovered inter CTC heterogeneity for transcripts, which are associated with response or resistance to endocrine therapy (e.g., ESR1, HER2, FGFR1). Hierarchical clustering revealed CTC subpopulations with different expressions of transcripts regarding the CTCs’ differential phenotypes (EpCAM, CD44, CD24, MYC, MUC1) and of transcripts involved in endocrine signaling pathways (FOXO, PTEN). Moreover, ER-positive CTCs exhibited significant higher expression of Cyclin D1, which might be relevant for CDK4/6 inhibitor therapies. Overall, gene expression profiles of uncultured and cultured CTCs resulted in a partly combined grouping. Our findings demonstrate that multi-marker RNA profiling of enriched single uncultured CTCs and cultured CTCs form cryopreserved DLA samples may provide important insights into intra-patient heterogeneity relevant for targeted therapies and therapy resistance.
Nuclear Phosphoinositides—Versatile Regulators of Genome Functions
Authors: Castano Enrique , Yildirim Sukriye , Fáberová Veronika , Krausová Alžběta , Uličná Lívia , Paprčková Darina , Sztacho Martin , Hozák Pavel
Published: June 2019

The many functions of phosphoinositides in cytosolic signaling were extensively studied; however, their activities in the cell nucleus are much less clear. In this review, we summarize data about their nuclear localization and metabolism, and review the available literature on their involvements in chromatin remodeling, gene transcription, and RNA processing. We discuss the molecular mechanisms via which nuclear phosphoinositides, in particular phosphatidylinositol (4,5)-bisphosphate (PI(4,5)P2), modulate nuclear processes. We focus on PI(4,5)P2’s role in the modulation of RNA polymerase I activity, and functions of the nuclear lipid islets—recently described nucleoplasmic PI(4,5)P2-rich compartment involved in RNA polymerase II transcription. In conclusion, the high impact of the phosphoinositide–protein complexes on nuclear organization and genome functions is only now emerging and deserves further thorough studies.
Biofunctionalization of Textile Materials.1. Biofunctionalization of Poly(Propylene) (PP) Nonwovens Fabrics by Alafosfalin
Authors: Kudzin Marcin H. , Mrozińska Zdzisława , Walawska Anetta , Sójka-Ledakowicz Jadwiga
Published: June 2019

This paper presents the method of obtaining poly(propylene) (PP) nonwoven fabrics with antimicrobial properties, using Alafosfalin as the nonwoven modifying agent. Alafosfalin, namely L-alanyl-L-1-aminoethylphosphonic acid, presents representative P-terminal phosphonodipeptide, which possesses a strong, broad spectrum of antimicrobial properties. The analysis of these biofunctionalized nonwoven fabrics processed by the melt-blown technique, included: scanning electron microscopy (SEM), UV/Vis transmittance, FTIR spectrometry, and air permeability. The nonwovens were subjected to microbial activity tests against colonies of Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. Results indicate that the described nonwovens can be successfully used as an antibacterial material.
Microbiological and Physicochemical Properties of Meat Coated with Microencapsulated Mexican Oregano (Lippia graveolens Kunth) and Basil (Ocimum basilicum L.) Essential Oils Mixture
Authors: Hernández-Hernández Elvia , Castillo-Hernández Gustavo , González-Gutiérrez Claudia J. , Silva-Dávila Areli J. , Gracida-Rodríguez Jorge N. , García-Almendárez Blanca E. , Di Pierro Prospero , Vázquez-Landaverde Pedro , Regalado-González Carlos
Published: June 2019

Microencapsulated essential oils (EOs) are increasingly used to protect the safety of foods due to their natural origin. The aim of this work was to determine the chemical composition of Mexican oregano (Lippia graveolens Kunth) (MOEO) and basil (Ocimum basilicum L.) (BEO) EOs, their combined effect against E. coli O157:H7, Lactobacillus plantarum, Brochothrix thermosphacta and Pseudomonas fragi, and their effect on microbiological and physicochemical properties of coated pork meat. EOs chemical composition was determined by GC/MS, their microencapsulated mixture (4 mg MOEO/mL/11 mg BEO/mL) was added to a filmogenic dispersion. Fluorescent probes were used to study the antimicrobial filmogenic active dispersion (FD) effect. Pork meat pieces were coated without microencapsulated EOs (CC), using FD (AC), or uncoated (C), vacuum packed and stored 28 days at 4 °C. Thymol (28.9%) and linalool (23.7%) were the major components of MOEO and BEO, respectively. The cell membrane of all bacteria was damaged by contact with FD. FD-coated samples (AC) exhibited the lowest concentration of 2-thiobarbituric acid reacting substances (TBARS) (0.027 ± 0.001 mg malonaldehyde/kg meat) and natural microbiota growth, while odor and color were the most accepted by untrained judges (range > 6). Coatings added with microencapsulated EOs mixture are a natural food preservation alternative to increase the shelf life of refrigerated meat products.
Transdermal Optical Wireless Links with Multiple Receivers in the Presence of Skin-Induced Attenuation and Pointing Errors
Authors: Varotsos George K. , Nistazakis Hector E. , Aidinis Konstantinos , Jaber F. , Rahman K.K. Mujeeb
Published: June 2019

The last few years, the scientific field of optical wireless communications (OWC) has witnessed tremendous progress, as reflected in the continuous emergence of new successful high data rate services and variable sophisticated applications. One such development of vital research importance and interest is the employment of high speed, robust, and energy-effective transdermal optical wireless (TOW) links for telemetry with implantable medical devices (IMDs) that also have made considerable progress lately for a variety of medical applications, mainly including neural recording and prostheses. However, the outage performance of such TOW links is significantly degraded due to the strong attenuation that affects the propagating information-bearing optical signal through the skin, along with random misalignments between transmitter and receiver terminals, commonly known as pointing error effect. In order to anticipate this, in this work we introduce a SIMO TOW reception diversity system that employs either OOK or more power-effective L-PPM schemes. Taking into account the joint impact of skin-induced attenuation and non-zero boresight pointing errors, modeled through the suitable Beckmann distribution, novel closed-form mathematical expressions for the average BER of the total TOW system are derived. Thus, the possibility of enhancing the TOW availability by using reception diversity configurations along with the appropriate modulation format is investigated. Finally, the corresponding numerical results are presented using the new derived theoretical outcomes.
Mechanochemical Reactions of Lithium Niobate Induced by High-Energy Ball-Milling
Authors: Kocsor Laura , Péter László , Corradi Gábor , Kis Zsolt , Gubicza Jenő , Kovács László
Published: June 2019

Lithium niobate (LiNbO3, LN) nanocrystals were prepared by ball-milling of the crucible residue of a Czochralski grown congruent single crystal, using a Spex 8000 Mixer Mill with different types of vials (stainless steel, alumina, tungsten carbide) and various milling parameters. Dynamic light scattering and powder X-ray diffraction were used to determine the achieved particle and grain sizes, respectively. Possible contamination from the vials was checked by energy-dispersive X-ray spectroscopy measurements. Milling resulted in sample darkening due to mechanochemical reduction of Nb (V) via polaron and bipolaron formation, oxygen release and Li2O segregation, while subsequent oxidizing heat-treatments recovered the white color with the evaporation of Li2O and crystallization of a LiNb3O8 phase instead. The phase transformations occurring during both the grinding and the post-grinding heat treatments were studied by Raman spectroscopy, X-ray diffraction and optical reflection measurement, while the Li2O content of the as-ground samples was quantitatively measured by coulometric titration.
Ferroelectric Self-Poling in GeTe Films and Crystals
Authors: Kriegner Dominik , Springholz Gunther , Richter Carsten , Pilet Nicolas , Müller Elisabeth , Capron Marie , Berger Helmut , Holý Václav , Dil J. Hugo , Krempaský Juraj
Published: June 2019

Ferroelectric materials are used in actuators or sensors because of their non-volatile macroscopic electric polarization. GeTe is the simplest known diatomic ferroelectric endowed with exceedingly complex physics related to its crystalline, amorphous, thermoelectric, and—fairly recently discovered—topological properties, making the material potentially interesting for spintronics applications. Typically, ferroelectric materials possess random oriented domains that need poling to achieve macroscopic polarization. By using X-ray absorption fine structure spectroscopy complemented with anomalous diffraction and piezo-response force microscopy, we investigated the bulk ferroelectric structure of GeTe crystals and thin films. Both feature multi-domain structures in the form of oblique domains for films and domain colonies inside crystals. Despite these multi-domain structures which are expected to randomize the polarization direction, our experimental results show that at room temperature there is a preferential ferroelectric order remarkably consistent with theoretical predictions from ideal GeTe crystals. This robust self-poled state has high piezoelectricity and additional poling reveals persistent memory effects.
On-Demand Processing of Data Cubes from Satellite Image Collections with the gdalcubes Library
Authors: Appel Marius , Pebesma Edzer
Published: June 2019

Earth observation data cubes are increasingly used as a data structure to make large collections of satellite images easily accessible to scientists. They hide complexities in the data such that data users can concentrate on the analysis rather than on data management. However, the construction of data cubes is not trivial and involves decisions that must be taken with regard to any particular analyses. This paper proposes on-demand data cubes, which are constructed on the fly when data users process the data. We introduce the open-source C++ library and R package gdalcubes for the construction and processing of on-demand data cubes from satellite image collections, and show how it supports interactive method development workflows where data users can initially try methods on small subsamples before running analyses on high resolution and/or large areas. Two study cases, one on processing Sentinel-2 time series and the other on combining vegetation, land surface temperature, and precipitation data, demonstrate and evaluate this implementation. While results suggest that on-demand data cubes implemented in gdalcubes support interactivity and allow for combining multiple data products, the speed-up effect also strongly depends on how original data products are organized. The potential for cloud deployment is discussed.
Seed and Seedling Detection Using Unmanned Aerial Vehicles and Automated Image Classification in the Monitoring of Ecological Recovery
Authors: Buters Todd , Belton David , Cross Adam
Published: June 2019

Monitoring is a crucial component of ecological recovery projects, yet it can be challenging to achieve at scale and during the formative stages of plant establishment. The monitoring of seeds and seedlings, which represent extremely vulnerable stages in the plant life cycle, is particularly challenging due to their diminutive size and lack of distinctive morphological characteristics. Counting and classifying seedlings to species level can be time-consuming and extremely difficult, and there is a need for technological approaches offering restoration practitioners with fine-resolution, rapid and scalable plant-based monitoring solutions. Unmanned aerial vehicles (UAVs) offer a novel approach to seed and seedling monitoring, as the combination of high-resolution sensors and low flight altitudes allow for the detection and monitoring of small objects, even in challenging terrain and in remote areas. This study utilized low-altitude UAV imagery and an automated object-based image analysis software to detect and count target seeds and seedlings from a matrix of non-target grasses across a variety of substrates reflective of local restoration substrates. Automated classification of target seeds and target seedlings was achieved at accuracies exceeding 90% and 80%, respectively, although the classification accuracy decreased with increasing flight altitude (i.e., decreasing image resolution) and increasing background surface complexity (increasing percentage cover of non-target grasses and substrate surface texture). Results represent the first empirical evidence that small objects such as seeds and seedlings can be classified from complex ecological backgrounds using automated processes from UAV-imagery with high levels of accuracy. We suggest that this novel application of UAV use in ecological monitoring offers restoration practitioners an excellent tool for rapid, reliable and non-destructive early restoration trajectory assessment.
Bayesian Analysis of Coefficient Instability in Dynamic Regressions
Authors: Ciapanna Emanuela , Taboga Marco
Published: June 2019

This paper deals with instability in regression coefficients. We propose a Bayesian regression model with time-varying coefficients (TVC) that allows to jointly estimate the degree of instability and the time-path of the coefficients. Thanks to the computational tractability of the model and to the fact that it is fully automatic, we are able to run Monte Carlo experiments and analyze its finite-sample properties. We find that the estimation precision and the forecasting accuracy of the TVC model compare favorably to those of other methods commonly employed to deal with parameter instability. A distinguishing feature of the TVC model is its robustness to mis-specification: Its performance is also satisfactory when regression coefficients are stable or when they experience discrete structural breaks. As a demonstrative application, we used our TVC model to estimate the exposures of S&P 500 stocks to market-wide risk factors: We found that a vast majority of stocks had time-varying exposures and the TVC model helped to better forecast these exposures.
A University Foreign Language Curriculum for Pre-Service Non-Language Subject Teacher Education
Authors: Borzova Elena , Shemanaeva Maria
Published: June 2019

The article focuses on the problems of teaching foreign languages (FL) to pre-service subject (other than foreign languages) teachers and highlights their possible solutions. A sustained discontent of both university teachers and students with the outcomes of FL classes gave impetus for a systematic, detailed study of the university FL education of the target group of learners. There was an acute need to develop well-grounded guidelines for the related course objectives, its content and adequate techniques intended for the target group of learners. The authors claim that the generally accepted English for Specific Purposes (ESP) or Content and Language Integrated Learning (CLIL) models of university FL education cannot be applied in this context for a number of reasons. A limited amount of classroom time, lack of updated teaching materials, mixed-ability classrooms, and unclear perspectives of the future application of the FL skills made the study urgent. Therefore, the teachers of the English Language Department of the Petrozavodsk State University (Russia) developed a project that is described in the article; 152 students and six teachers participated in its implementation. The objective of the project was to introduce reasonable changes into the approaches, the aims, the content, and the techniques for rational and effective FL teaching and learning in this context. The authors identified priorities of the university FL education in line with the context and the target learner characteristics. The article presents arguments in favor of the introduced changes, describes the phases of the project work, and gives details of all the constituents of the proposed curriculum. Based on the outcomes of the project, the authors conclude that it is necessary to undertake further research steps in this direction.
The Socio-Linguistic Adaptation of Migrants: The Case of Oralman Students’ Studying in Kazakhstan
Authors: Valieva Fatima , Sagimbayeva Jannat , Kurmanayeva Dina , Tazhitova Gulzhakhan
Published: June 2019

This article examines different aspects of the oralman students’ socio-linguistic adaptation. Scientific research has identified various obstacles oralmen face when studying at Universities in Kazakhstan, especially in the context of the English language studying process. The data used in this paper explores certain peculiarities of oralman students’ adaptation to new educational environments, their attitude towards English, and their difficulties in advancing in foreign language learning. The electronic questionnaires, which included the Likert scale, multiple choice and open-ended questions, were offered to 600 students, while interviews were conducted with 90 students in the last stage of the research. Descriptive statistics and one-way analysis of variance were used to analyze the data. The scientific findings indicated some unexpected difficulties in the oralman students’ socio-linguistic adaptation, which decreased their motivation for learning the foreign language and reduced effectiveness of the teaching. However, certain noticeable discrepancies between the anticipated and real educational context were revealed. The interview and data analysis made it possible to uncover cultural, linguistic, social, and psychological problems. The scientific research suggests that the incorporation of some integrative effective methods and techniques into English classes would be useful for improvement of the oralman students’ English language skills.
A Machine Learning Approach to Fitting Prescription for Hearing Aids
Authors: Mondol S I M M Raton , Lee Sangmin
Published: June 2019

A successful Hearing-Aid Fitting (HAF) is more than just selecting an appropriate Hearing Aid (HA) device for a patient with Hearing Loss (HL). The initial fitting is given by the prescription based on user’s hearing loss; however, it is often necessary for the audiologist to readjust some parameters to satisfy the user demands. Therefore, in this paper, we concentrated on a new application of Neural Network (NN) combined with a Transfer Learning (TL) strategy to develop a fitting algorithm with the prescription database for hearing loss and readjusted gain to minimize the gap between fitting satisfaction. As prior information, we generated the data set from two popular hearing-aid fitting software, then fed the training data to our proposed model, and verified the performance of the architecture. Pondering real life circumstances, where numerous fitting records may not always be accessible, we first investigated the number of minimum fitting records required for possible sufficient training. After that, we evaluated the performance of the proposed algorithm in two phases: (a) NN with refined hyper parameter showed enhanced performance in compare to state-of-the-art DNN approach, and (b) the TL approach boosted the performance of the NN algorithm in a broad way. Altogether, our model provides a pragmatic and promising tool for HAF.
A Method for the Development of Dynamic Site Index Models Using Height–Age Data from Temporal Sample Plots
Authors: Socha Jarosław , Tymińska-Czabańska Luiza
Published: June 2019

Knowledge of the potential productivity of forest sites is fundamental for making strategic decisions in forest management. Site productivity is usually evaluated using the site index, and therefore the development of site index models is one of the crucial tasks in forest research and forest management. This research aims to develop an effective method for building top-growth and site index models using data from temporary sample plots (TSP). Exploiting the advantages of the generalised algebraic difference approach (GADA), the proposed method overcomes the limitations of the guide curve method that has been to date used in site index modelling using TSPs data and allows to obtain only a set of anamorphic site index curves. The proposed approach enables the construction of dynamic site index models with polymorphism and variable asymptotes. Such models better reflect local, site-specific height growth trajectories and therefore allow more appropriate site index estimation. We tested the proposed method using data collected from 5105 temporary sample plots in Poland. Our results indicate that growth trend estimates using height–age measurements of TSPs may be valuable data for modelling top height growth. For these reasons, the proposed method can be very useful in forest management.
Estimating Canopy Fuel Attributes from Low-Density LiDAR
Authors: Engelstad Peder S. , Falkowski Michael , Wolter Peter , Poznanovic Aaron , Johnson Patty
Published: June 2019

Simulations of wildland fire risk are dependent on the accuracy and relevance of spatial data inputs describing drivers of wildland fire, including canopy fuels. Spatial data are freely available at national and regional levels. However, the spatial resolution and accuracy of these types of products often are insufficient for modeling local conditions. Fortunately, active remote sensing techniques can produce accurate, high-resolution estimates of forest structure. Here, low-density LiDAR and field-based data were combined using randomForest k-nearest neighbor imputation (RF-kNN) to estimate canopy bulk density, canopy base height, and stand age across the Boundary Waters Canoe Area in Minnesota, USA. RF-kNN models produced strong relationships between estimated canopy fuel attributes and field-based data for stand age (Adj. R2 = 0.81, RMSE = 10.12 years), crown fuel base height (Adj. R2 = 0.78, RMSE = 1.10 m), live crown base height (Adj. R2 = 0.7, RMSE = 1.60 m), and canopy bulk density (Adj. R2 = 0.48, RMSE = 0.09kg/m3). These results suggest that low-density LiDAR can help estimate canopy fuel attributes in mixed forests, with robust model accuracies and high spatial resolutions compared to currently utilized fire behavior model inputs. Model map outputs provide a cost-efficient alternative for data required to simulate fire behavior and support local management.
RETRACTED: Ahmed et al. Dual Solutions in a Boundary Layer Flow of a Power Law Fluid over a Moving Permeable Flat Plate with Thermal Radiation, Viscous Dissipation and Heat Generation/Absorption. Fluids 2018, 3, 6
Authors:   Fluids Editorial Office
Published: June 2019

This article [...]
Novel Molecular Synapomorphies Demarcate Different Main Groups/Subgroups of Plasmodium and Piroplasmida Species Clarifying Their Evolutionary Relationships
Authors: Sharma Rahul , Gupta Radhey S.
Published: June 2019

The class Hematozoa encompasses several clinically important genera, including Plasmodium, whose members cause the major life-threating disease malaria. Hence, a good understanding of the interrelationships of organisms from this class and reliable means for distinguishing them are of much importance. This study reports comprehensive phylogenetic and comparative analyses on protein sequences on the genomes of 28 hematozoa species to understand their interrelationships. In addition to phylogenetic trees based on two large datasets of protein sequences, detailed comparative analyses were carried out on the genomes of hematozoa species to identify novel molecular synapomorphies consisting of conserved signature indels (CSIs) in protein sequences. These studies have identified 79 CSIs that are exclusively present in specific groups of Hematozoa/Plasmodium species, also supported by phylogenetic analysis, providing reliable means for the identification of these species groups and understanding their interrelationships. Of these CSIs, six CSIs are specifically shared by all hematozoa species, two CSIs serve to distinguish members of the order Piroplasmida, five CSIs are uniquely found in all Piroplasmida species except B. microti and two CSIs are specific for the genus Theileria. Additionally, we also describe 23 CSIs that are exclusively present in all genome-sequenced Plasmodium species and two, nine, ten and eight CSIs which are specific for members of the Plasmodium subgenera Haemamoeba, Laverania, Vinckeia and Plasmodium (excluding P. ovale and P. malariae), respectively. Additionally, our work has identified several CSIs that support species relationships which are not evident from phylogenetic analysis. Of these CSIs, one CSI supports the ancestral nature of the avian-Plasmodium species in comparison to the mammalian-infecting groups of Plasmodium species, four CSIs strongly support a specific relationship of species between the subgenera Plasmodium and Vinckeia and three CSIs each that reliably group P. malariae with members of the subgenus Plasmodium and P. ovale within the subgenus Vinckeia, respectively. These results provide a reliable framework for understanding the evolutionary relationships among the Plasmodium/Piroplasmida species. Further, in view of the exclusivity of the described molecular markers for the indicated groups of hematozoa species, particularly large numbers of unique characteristics that are specific for all Plasmodium species, they provide important molecular tools for biochemical/genetic studies and for developing novel diagnostics and therapeutics for these organisms.
Cloudy with a Chance of Insights: Context Dependent Gene Regulation and Implications for Evolutionary Studies
Authors: Buchberger Elisa , Reis Micael , Lu Ting-Hsuan , Posnien Nico
Published: June 2019

Research in various fields of evolutionary biology has shown that divergence in gene expression is a key driver for phenotypic evolution. An exceptional contribution of cis-regulatory divergence has been found to contribute to morphological diversification. In the light of these findings, the analysis of genome-wide expression data has become one of the central tools to link genotype and phenotype information on a more mechanistic level. However, in many studies, especially if general conclusions are drawn from such data, a key feature of gene regulation is often neglected. With our article, we want to raise awareness that gene regulation and thus gene expression is highly context dependent. Genes show tissue- and stage-specific expression. We argue that the regulatory context must be considered in comparative expression studies.
Super-Resolution Microscopy of Chromatin
Authors: Birk Udo J.
Published: June 2019

Since the advent of super-resolution microscopy, countless approaches and studies have been published contributing significantly to our understanding of cellular processes. With the aid of chromatin-specific fluorescence labeling techniques, we are gaining increasing insight into gene regulation and chromatin organization. Combined with super-resolution imaging and data analysis, these labeling techniques enable direct assessment not only of chromatin interactions but also of the function of specific chromatin conformational states.
Land Use and Land Cover Changes in the Owabi Reservoir Catchment, Ghana: Implications for Livelihoods and Management
Authors: Antwi-Agyei Philip , Kpenekuu Felix , Hogarh Jonathan N. , Obiri-Danso Kwasi , Abaidoo Robert C. , Jeppesen Erik , Andersen Mathias Neumann
Published: June 2019

Reservoir catchments in Ghana have undergone significant changes in recent years with major implications for socio-economic development and local livelihoods. We studied land use and land cover changes and their impacts on livelihoods in the Owabi reservoir catchment from 1970 to 2014 using Landsat, ERDAS Imagine and Arc Geographic Information System (ArcGIS 10.2) software supplemented with participatory approaches including focus group discussions, key informant interviews and questionnaire surveys with 400 households. Our results showed that, since 1970, 24.6% of high-density forests and 15.8% of sparse forests have disappeared, while the built-up area has increased from 9.8% to 56.6%. Additionally, the proportion of bare soil (areas that do not have vegetation cover due to forest clearing and other anthropogenic activities) has increased, while the areas of waterbodies have declined. We identified urbanisation and lack of community involvement in catchment management as the key factors driving the land cover changes that have adversely affected the livelihoods of the local fringe communities. This study highlights the threats from urbanisation to land cover changes and identifies the key drivers of land use change. For effective and sustainable management of natural resources, the local communities should be more actively involved in the decision-making process regarding the management of their individual catchments.
Changes in the Nature of Long-Term Fluctuations of Water Flow in the Subarctic Region of Yakutia: A Global Warming Perspective
Authors: Shpakova Raisa , Kusatov Konstantin , Mustafin Sabir , Trifonov Alexander
Published: June 2019

Global warming has begun to affect Yakutia, an area recognized as the coldest region of the Northern Hemisphere. Previous research has indicated that the effects of global warming will be long-term. When modeling oncoming climatic changes, researchers often forecast the related water flow changes in various water bodies as well. However, these evaluations frequently differ from the actual water flow data. Thus, the current study identifies and assesses the trends in long-term flow fluctuations in the current context of global warming. This is particularly relevant in the subarctic region of Yakutia, because the local climate is not significantly influenced by anthropogenic factors. The region has an essentially uniform climate, and the river basins within the subarctic zone flow in the same direction. Thus, the study parameters can be adequately compared. Analysis of changes in the water regimen parameters of the rivers in this region is of particular importance. This study demonstrates that the changes in the long-term river regimen in the region, within approximately equivalent climate zones, have been highly and locally variable indifferent areas and time periods. However, we were unable to detect any specific consistency in these changes. The water content of almost all rivers in Yakutia has increased in the last 30 years (approximately), thus confirming general assumptions based on predictive models of climate changes; however, in most cases, such changes were the result of reaching the high-water stage of established long-term cycles. The nature of long-term fluctuations in the water flow of rivers did not change in about half of the Yakutia rivers. One water body showed a further decrease in the water content from the norm, both in terms of duration and water flow rate. Meanwhile, specific water bodies exhibited extreme long-term fluctuations, which are predicted to be a reaction to global warming. Prior to the onset of significant warming in the region, the trends of long-term water discharge fluctuations were stationary. Then, the trends of certain rivers became non-stationary due to the reasons indicated above. On their own, quantitative characteristics are insufficient to evaluate actual changes in water regimens. Moreover, evaluations obtained in the absence of a trend analysis of specific long-term discharge fluctuations, which can only be performed via graphic visualization, are most likely to be inaccurate.
Impact of CSR-Relevant News on Stock Prices of Companies Listed in the Austrian Traded Index (ATX)
Authors: Ender Manuela , Brinckmann Finn
Published: June 2019

This paper examines the short-term influence of CSR-relevant news on the enterprise value in the form of respective shareholder value, which is represented by the corresponding stock price. This effect is measured using an event study applied to companies in the “Austrian Traded Index” (ATX). Subsequently, the results of the study show that CSR-relevant news in the financial portal finanzen.net trigger statistically significant abnormal returns and average cumulated abnormal returns within a period of five days. Based on these research results, it is stated that CSR-relevant news has a significant impact on the shareholder value of a company represented by its stock price. The hypotheses defined on the basis of the previously conducted studies on this topic were only partially confirmed. In particular, the negative effects of negative CSR-relevant news are insufficiently shown. Based on these findings, it can be deduced that the effects of the news are reinforced by the CSR relevance, although media presence due to the publications is a factor to be explored.
Tonantzin, a New Genus of Bess Beetle (Coleoptera, Passalidae) from a Montane Subtropical Forest in Central Mexico, with a Review of the Taxonomic Significance of the Mesofrontal Structure in Proculini
Authors: Beza-Beza Cristian Fernando , Jiménez-Ferbans Larry , Clarke Dave J. , Reyes-Castillo Pedro , McKenna Duane D.
Published: June 2019

Mexico has the third highest diversity of passalid beetles in the World. Here we describe Tonantzin new genus, a new monotypic genus, potentially endemic to the mountains of central Mexico. The new genus is diagnosed by a new configuration of characters from the mesofrontal structure (MFS) in addition to other characters. The MFS in Passalidae has been treated either as a composite complex character or a combination of individual characters. Using a broad taxonomic sample within Proculini, we discuss the taxonomic and systematic implications of the MFS for the tribe. We define the MFS type tepetl. Given the importance of the MFS for passalid taxonomy we propose a new delimitation of the structure using boundaries based on internal and external head structures. We argue that the treatment of the MFS as a complex character better captures the nature of this structure but we ultimately find a need to standardize the way in which this structure is described in the taxonomic literature and used in phylogenetic analyses.
Carry-Over Niches for Lepidopteran Maize Stemborers and Associated Parasitoids during Non-Cropping Season
Authors: Sokame Bonoukpoè Mawuko , Rebaudo François , Musyoka Boaz , Obonyo Julius , Mailafiya Duna Madu , Le Ru Bruno Pierre , Kilalo Dora Chao , Juma Gerald , Calatayud Paul-André
Published: June 2019

Sources of infestation are the key elements to be considered in the development of habitat management techniques for the control of maize stemborers. Several wild plants, grasses mostly, have been identified that serve as hosts for stemborers and their parasitoids during the off-season when maize is not present in the field. However, their abundance is much lower in wild plants compared to cultivated fields. Thus, the role of wild plants as a reservoir for cereal stemborers and their parasitoids is still controversial, particularly in agro-ecosystems with reduced wild habitat. We studied the occurrence of different maize stemborers and associated parasitoids in maize stem residues and wild grasses during non-cropping seasons as potential carry-over populations to subsequent early season maize plants. Surveys were conducted in the central region of Kenya during long and short dry seasons in maize residues and wild grasses as well as during the two rainy seasons in maize plants at earlier and late whorl stages during the years of 2017 and 2018. Wild habitat had a higher species diversity than maize residues habitat, but maize residues had a higher abundance of maize stemborer species, such as Busseola fusca, Sesamia calamistis, and Chilo partellus, and of associated parasitoid species (i.e., Cotesia flavipes and Cotesia sesamiae) than wild plants. Our surveys, complemented by field parasitoid releases of C. flavipes and C. sesamiae, indicated that maize residues constitute a better refugia reservoir not only of the maize stemborers but also of C. flavipes and C. sesamiae during non-cropping seasons as compared to wild plants and, thus, might constitute in this region the main source of both stemborers and C. flavipes/C. sesamiae carry-over in maize plants during the subsequent cropping season. Thus, systematic destruction of maize residues would not help the biological control of lepidopteran stemborers. This is particularly true in areas with reduced wild habitat.
Prevalence of Function-Dependent Temporomandibular Joint and Masticatory Muscle Pain, and Predictors of Temporomandibular Disorders among Patients with Lyme Disease
Authors: Osiewicz Magdalena , Manfredini Daniele , Biesiada Grażyna , Czepiel Jacek , Garlicki Aleksander , Aarab Ghizlane , Pytko-Polończyk Jolanta , Lobbezoo Frank
Published: June 2019

The aim was to determine the occurrence of temporomandibular disorders (TMDs) in patients with Lyme disease (LD), and to estimate the contribution of factors that may identify TMD among LD patients. In seventy-six (N = 76) adult patients with LD (mean age 57.6 ± 14.6 years) and 54 healthy non-Lyme volunteers with a mean age of 56.4 ± 13.5 years, possible function (i.e., non-pain) diagnoses were established using the Research Diagnostic Criteria of Temporomandibular Disorders (RDC/TMD). Pain diagnoses were established by means of the function-dependent dynamic and static tests. The two groups did not significantly differ in the frequency of disc displacements diagnoses and function-dependent pain diagnoses. LD showed a significantly higher frequency (p < 0.001) of osteoarthrosis than the control group. For the prediction of pain diagnoses in LD patients, the single regression analyses pointed out an association with age, sleep bruxism (SB), and awake bruxism (AB). Two predictors (i.e., SB (p = 0.002) and AB (p = 0.017)) were statistically significant in the final multiple variable model. The frequency of TMD in patients with LD based on function-dependent tests was not significantly different from that in the control group. This investigation suggests that the contribution of bruxism to the differentiation between patients with Lyme and TMD is high.
The Bruxoff Device as a Screening Method for Sleep Bruxism in Dental Practice
Authors: Saczuk Klara , Lapinska Barbara , Wilmont Paulina , Pawlak Lukasz , Lukomska-Szymanska Monika
Published: June 2019

Sleep bruxism (SB) is a masticatory muscle activity during sleep and a common phenomenon. Severe SB can have a serious impact on the success of dental treatment. Reliable methods of screening and diagnosing patients with SB are crucial. Therefore, in this study, a Bruxoff device as a potential screening and diagnostic method for sleep bruxism was evaluated. A total of 60 adults participated in this study: 35 patients with symptoms of bruxism (study group) and 25 asymptomatic patients (control group). Data were recorded using Bruxoff. All symptomatic patients participating in the study turned out to be bruxers, while not all asymptomatic patients turned out to be non-bruxers according to the Bruxoff device. Bruxoff is a simple screening device that can be safely used to evaluate masseter muscle activity during sleep. Since the device does not have a built-in microphone and/or video camera and, therefore, cannot record grinding sounds, the objective capabilities of Bruxoff as a single diagnostic device for sleep bruxism are limited.
The World Association against Infection in Orthopaedics and Trauma (WAIOT) procedures for Microbiological Sampling and Processing for Periprosthetic Joint Infections (PJIs) and other Implant-Related Infections
Authors: Drago Lorenzo , Clerici Pierangelo , Morelli Ilaria , Ashok Johari , Benzakour Thami , Bozhkova Svetlana , Alizadeh Chingiz , del Sel Hernán , Sharma Hemant K , Peel Trisha , Mattina Roberto , Romanò Carlo Luca
Published: June 2019

While implant-related infections continue to play a relevant role in failure of implantable biomaterials in orthopaedic and trauma there is a lack of standardised microbiological procedures to identify the pathogen(s). The microbiological diagnosis of implant-related infections is challenging due to the following factors: the presence of bacterial biofilm(s), often associated with slow-growing microorganisms, low bacterial loads, previous antibiotic treatments and, possible intra-operative contamination. Therefore, diagnosis requires a specific set of procedures. Based on the Guidelines of the Italian Association of the Clinical Microbiologists (AMCLI), the World Association against Infection in Orthopaedics and Trauma has drafted the present document. This document includes guidance on the basic principles for sampling and processing for implant-related infections based on the most relevant literature. These procedures outline the main microbiological approaches, including sampling and processing methodologies for diagnostic assessment and confirmation of implant-related infections. Biofilm dislodgement techniques, incubation time and the role of molecular approaches are addressed in specific sections. The aim of this paper is to ensure a standardised approach to the main microbiological methods for implant-related infections, as well as to promote multidisciplinary collaboration between clinicians and microbiologists.
Targeting Tyrosine Phosphatases by 3-Bromopyruvate Overcomes Hyperactivation of Platelets from Gastrointestinal Cancer Patients
Authors: Faria Alessandra V. S. , Andrade Sheila S. , Reijm Agnes N. , Spaander Manon C. W. , de Maat Moniek P. M. , Peppelenbosch Maikel P. , Ferreira-Halder Carmen V. , Fuhler Gwenny M.
Published: June 2019

Venous thromboembolism (VTE) is one of the most common causes of cancer related mortality. It has been speculated that hypercoagulation in cancer patients is triggered by direct or indirect contact of platelets with tumor cells, however the underlying molecular mechanisms involved are currently unknown. Unraveling these mechanisms may provide potential avenues for preventing platelet-tumor cell aggregation. Here, we investigated the role of protein tyrosine phosphatases in the functionality of platelets in both healthy individuals and patients with gastrointestinal cancer, and determined their use as a target to inhibit platelet hyperactivity. This is the first study to demonstrate that platelet agonists selectively activate low molecular weight protein tyrosine phosphatase (LMWPTP) and PTP1B, resulting in activation of Src, a tyrosine kinase known to contribute to several platelet functions. Furthermore, we demonstrate that these phosphatases are a target for 3-bromopyruvate (3-BP), a lactic acid analog currently investigated for its use in the treatment of various metabolic tumors. Our data indicate that 3-BP reduces Src activity, platelet aggregation, expression of platelet activation makers and platelet-tumor cell interaction. Thus, in addition to its anti-carcinogenic effects, 3-BP may also be effective in preventing platelet-tumor cell aggregationin cancer patients and therefore may reduce cancer mortality by limiting VTE in patients.