bluuebunny/crossref_metadata_2025 huggingface dataset:
Application of Surfagon and Ketaprofen for increasing fertility and preventing embryonic death in cows after insemination
Authors: Kraevskiy A.Y. , Sokolyuk V.M. , Travetskyi M.O.
Published: December 2020

Nowadays only 30–50% of inseminated cows actually calve and the majority of pregnancy loses happens during the first 2–3 weeks after insemination. Fertility rate of heifers and cows with average milk production is from 90% to 100%, but in high producing animals that are influenced by stress, such rate can be reduced to 80%. Our experimental researches were conducted in conditions of dairy farms of Poltava and Kharkiv regions of Ukraine in summer of 2020. Estrus synchronizing in cows was started from 50-60 days after calving in Summer time. To prevent Infectious Rinotracheitis, Viral Diarrhea, Parainfluenza 3 in dairy herds cows were vaccinated with polyvalent vaccinations usually before or at the beginning of dry period. Before estrus synchronization trans-rectal sonographic examination was done in all cows to determine condition of uterus and ovaries. During the trial the herd of cows was divided in two groups depending on length of period from calving to estrus synchronizing. In the first group were cows up to 90 days after calving, in the second group – 91 and more days. We registered that Estrus synchronization accordingly to OVSYNCH protocol in 50-90 days (first group) and 91 and more days (second group) after the calving and using Surfagon on day 5 after insemination promote an increasing of cows fertility in the second group on 5.8% compare to control. At the same time combined using of Surfagon on 5-th and Ainil on 11-th day after insemination in cows of first group promotes their fertility on 7.5% compare to control animals of this group, and in the second group this increased on 12.6%. At the time of repeated pregnancy diagnostic of cows in 60-65 days there was established absence of previously determined pregnancy in 5.0-11.0% of animals, that was the evidence of late embryonic death. When Surfagon was used in cows on day 5 after insemination frequency of embryonic death was decreased in 1.7 times or by 4.7% compare to the control group of cows. Combined using of Surfagon on day 5 and Ainil on day 11 promoted decreasing of embryonic death in 2.3 times or by 6.4% (p≥0.05).
Disinvasive action of aldehyde and chlorine disinfectants on the test-culture of Toxocara canis eggs
Authors: Paliy A.P. , Sumakova N.V. , Rodionova K.O.
Published: December 2020

Disinfection of environmental objects with highly effective disinfectants is a reliable and effective means of preventing the occurrence of outbreaks of infectious and parasitic diseases. The purpose of our work was to determine the disinfection properties of modern disinfectants based on the test culture of Toxocara canis helminths and to establish the optimal modes of their use. It has been proven that an aldehyde disinfectant containing didecyldimethylammonium chloride (2.25%), benzalkonium chloride (8.0%), glutaraldehyde (15.0%), phosphoric acid, nonionic surfactants, water exhibits disinvasive activity against test cultures of Toxocara canis eggs at a concentration of 2.0-4.0% at a temperature of 20±0.5°C and an exposure of 3-24 hours, and the ovocidal efficiency is from 90.60% to 99.70%. Aldehyde disinfectant can be used for disinfection of soil (black earth, sandy loam, loamy) contaminated with Toxocara canis eggs, at a concentration of 4.0% at 6:00 exposure and a consumption rate of 3000 cm3/m2. Chlorine agent, contains dichlorantin, dimethylhydantoin (12.4-16.4%), dispersant (9.0-12%), nonionic surfactants, corrosion inhibitor, filler exhibits disinvasive activity against Toxocara canis test culture in a concentration of 3.0-4.0% with an exposure of 3-24 hours, and the ovocidal efficiency in this case ranges from 97.40% to 98.82%. The chlorine agent is effective for soil disinfection only at a depth of up to 2 cm at a concentration of 4.0% at a consumption rate of 1000 cm3/m2 and an exposure time of 24 hours.
Selection of technological regime and cryoprotector for lyophilization of lactobacteria (Lactobacillus spp.)
Authors: Paliy A.P. , Gujvinska S.O. , Alrawashdeh M.S.
Published: December 2020

Despite the success achieved in the comprehensive study of probiotic cultures, today there are a number of problems associated with the low viability of lactic acid bacteria during their processing and long-term storage in probiotics. Our work aimed to select the optimal technological regime and cryoprotectant to preserve the viability of lactic acid bacteria Lactobacillus spp. during their lyophilization. According to the results of the conducted researches, it is established that for freeze-drying of probiotic cultures Lactobacillus spp. in the facility LZ-45.27 (Frigera, Czech Republic) the most optimal is the mode which provides a rise of temperature within 45 hours from minus 70.0±1.0 °C to plus 26.0±1.0 °C with a speed of 2.2±0.1 °C/hour. It is effective to use protective media for lactobacilli, which consist of: skim milk (90%) and sucrose (10%); skim milk (90%) and lactose (10%); skim milk (90%), glucose (2.5%), sucrose (2.5%), lactose (5.0%) (P≤0.05). Freeze-drying of lactic acid bacteria under optimal conditions and the addition of cryoprotectants will avoid the problems associated with a significant reduction in the number of microbial cells. The results of research can be used for long-term storage of cultures of lactobacilli by their lyophilization.
Improvement of storage resistance of sausage products to micellar mushrooms by synthetic protective shrinks
Authors: Rodionova K.O. , Palii A.P. , Paliy A.P.
Published: December 2020

The aim of the work was to establish the frequency of detection of mold of raw smoked sausages and to evaluate the use of synthetic protective shrinks, which provide long-term storage of sausages. Nine batches (310 samples) of raw smoked sausages were examined, 487 crops were made on special media, 154 samples of sausages were examined according to physical and chemical parameters. We determined that an intensive contamination of their surface with micellar fungi during the sale of raw smoked sausages in retail trade is taking place: Penicillium (62.9%), Aspergillus (18.2%), Mucor (10.7%), Cladosprium (4.5%). We found that on the 5th day of their sale 4.57±1.64% of experimental sausage bulks are contaminated with micellar fungi but more massive and intensive development of micromycelia was found on the 20th day of sale (29.32±8.16% of cases). We proved that the use of protective coating/srink “Optiglianets” (Lofink Group, Ukraine), “CoatStar 110” (OJSC “Upakprint”, Russia), “Polisved1” (OJSC “Omega”, Russia), “Antibak Tauchmassa” (Company “Swed Holding”, Russia) and “Tauchmasse KP-15”, Italy), delayed up to 20 days the development of micellar fungi on the surface of prototypes of smoked sausages. We applied them in a thin layer (0.06-0.10 mm depending on the applied means) on the surface of sausage bulks and dried in drying chambers at temperature of 15.0±2.0 °C and 45 minutes exposure at a relative humidity of 70%. The weight loss of raw smoked sausages was the lowest with the use of protective coating “Optiglyanets” (Lofink Group, Ukraine) and “Polisved-1” (OJSC “Omega”, Russia). It was 14.5±0.8% and equal to 60% reduction compared to other protective coatings/shrinks implementation.
Seasonal influence on biochemical blood parameters in males of Californian rabbit breed
Authors: Duda Y.V. , Prus M.P. , Shevchik R.S.
Published: December 2020

Biochemical parameters of rabbit blood are important for the control of animal diseases and the impact on meat productivity of diets and various supplements. The biochemical profile of blood changes under the influence of various factors, including temperature and humidity, which are associated with seasonality. The aim of the study was to determine the influence of seasonality on the biochemical analysis of blood of meat rabbits.The average annual temperature in the rabbitry was 15.9±1.10 °? during the observation period, with minimum values in winter +5.30 °?, maximum values in summer +27.70 °? and an average relative humidity of 68.3±1.2%. The seasonal fluctuations (within physiological values) of some serum indicators of protein-nitrogen metabolism in males of Californian rabbits breed have been established. In spring, we observed a significant increase in the concentration of total protein by 7.0-12.0% and albumin – by 9.7-9.8%. At the same time, the lowest values of urea, creatinine and uric acid were equal or less than the average annual physiological indicators. Deviations of temperature from comfortable indicators in winter and summer led to the adaptive changes in rabbit organisms. We revealed the changes in the creatinine econtent in summer, uric acid content in summer and winter and increased activity of ALAT enzyme in winter and summer. We suggested that in case of rabbit keeping in rooms without artificial regulation of the microclimate, it is necessary to consider the influence of seasonal temperatures on blood serum biochemical parameters.
Distribution of trematodes in waterfowl of Pavlodar Oblast (Kazakhstan)
Authors: Akhmetov K.K. , Maralbaeva D.G. , Matsyura A.V.
Published: December 2020

The article presents the parasitological studies of Trematoda, collected by complete helminthological dissections of birds in the Pavlodar region of the Republic of Kazakhstan. In Pavlodar region, we have currently diagnosed among the Trematoda, the presence of 16 species belonging to four subgenera and seven families. We supposed that there is a local pest-spot of Prosthogonimidae and Echinistomatidae in the north-east of Kazakhstan, because we have found marites in young migratory birds. We noted that the pest-spot of Echinostomatidae and Echinistomatidae (in years with their registration) in the territory of Pavlodar region was broad enough, because the trematode marites of these families were registered from the birds, sampled at the geographically remote waterbodies. We revealed that the hotspots of Prosthogonimidae localization varied, which was most likely determined by the annual climatic conditions.
Development of technical regulations for the capsulated probiotic manufacture
Authors: Paliy A.P. , Gujvinska S.O. , Kalashnyk M.V.
Published: December 2020

The aim of this work was to develop a technological regulation for the production of probiotic based on lacto- and bifidobacteria in the form of intestinal-soluble capsules for farm animals. Three experimental series of probiotic drug were made in the form of enteric capsules based on lactic acid bacteria. It has been established that the Bifidobacterium adolectentis No 17-316 strain and the Lactobacillus plantarum No 7-317 strain are the most adapted for the bacterial consortium. These strains have high biological activity and correspond to certain authentic characteristics. The pharmaceutical composition is proposed in the form of capsules with following ingredients: 50% of dry biomass of Lactobacillus plantarum No 7-317 and Bifidobacterium adolectentis No 17-316 strains, 5% of glucose, 5% of lactose, 9% of aerosil and 31% of starch. The content of lyophilized bacteria in one capsule is not less than 1 × 107 CFU of bifidobacteria/cm3 and 1 × 107 CFU of lactobacilli/cm3. A scheme of the manufacturing process of the drug in form of capsules includes the following stages: production of nutrient media and working solutions; growing cultures of lacto- and bifidobacteria, freeze drying of cultures of lacto- and bifidobacteria, obtaining a dry mass of lactic acid bacteria for encapsulation, encapsulation of the drug, control of the manufactured product before release, marking, packaging of capsules, transportation, storage of a drug.
Effect of agrotechnical and chemical practices on Fusarium rot root in sugar beet production
Authors: Maui A.A. , Aipeisova S.A. , Utarbaeva N.A. , Matsyura A.V.
Published: December 2020

Sugar beet is one of the economically significant industrial crops for the south and southeast of Kazakhstan. In the conditions of Kazakhstan, the best predecessors of sugar beet are winter wheat and alfalfa, which contribute to the improvement of the susceptibility of beet rot. Early plowing by the semi-pair type in the second decade of August allows reducing the susceptibility of root crops to Fusarium rot by 18%. It is shown that 6 centner/ha of wheat straw on the artificial background contributed to decrease in the prevalence of Fusarium rot, on average 1.4 times, Rhizoctoniosis rot – 1.2 times compared with the control. Increasing the rate of nitrogen and phosphate fertilizers contribute to the development of root rot (2-3 times) of sugar beet. To reduce the severity of rotten root crops (from 1.3 to 2.5 times), 6 t/ha of wheat straw are proposed to be applied to the soil together with N90P75K70. Seed treatment with tachigaren seed, rovral and fundazol reduces the development of rot by 8.1-16.6% and leads to an increase in yield of root crops by 31.5-50.2 c/ha.
Influence of organic and non-organic microelements on productivity and metabolic processes in growing young pigs
Authors: Chabaev M.G. , Nekrasov R.V. , Klementiev M.I.
Published: December 2020

The purpose of our research was to determine the effect two different complexes of elements of copper, zinc, iron, manganese, selenium by organic and mineral origin in feeding mixture “SK-4” on growing pigs productivity, metabolic state and mineral deposition in their organs. We formed two groups of growing pigs, 40 heads each, with an average live weight of 13.6 kg at the age of 42 days. In addition to the main diet, the experimental animals were fed by two premixes of enriched mineral (in the form of sulfates) and organic (as chelates) complex of trace elements. In growing period, the pigs from control and experimental groups were kept in equal conditions. Within 56 days of experiments the productivity of animals was depended on feeding, whereas the use of organic complex increased the average daily live weight gain by 6.3% (p≤0.01) with simultaneous decrease in exchange of energy costs by 6.4%. In the experimental group, the feeding with organic form of trace elements contributed to the increase in blood: erythrocytes - by 4.8% (p≤0.01) and hemoglobin - by 15% (p≤0.001) during the whole research period. We proved that the addition of chelate forms to the diet of growing pigs provided greater deposition of trace elements in their organs, which allowed reducing the excretion of trace elements, which is confirmed by the changes in histological sections. The results of our study showed that organic elements were more effective in terms of pigs production and health, and fully meet their needs in essential elements.
Anfibios y reptiles de un humedal periurbano: El Estero, San Ramón, Costa Rica
Authors: Núñez Melvin Cartín
Published: December 2020

Con el avance de la frontera agrícola, el crecimiento urbano y el cambio climático, muchas especies de anfibios y reptiles han visto reducidas sus poblaciones. Pocos trabajos se han hecho en el medio urbano con este grupo, en particular en Costa Rica, por lo que se realizó un primer esfuerzo por generar información de línea base en la periferia de la ciudad de San Ramón, Alajuela. Se eligió la microcuenca alta de la quebrada El Estero, ubicada al oeste de la ciudad. Allí se aplicaron varios métodos de muestreo como transectos y cuadrantes de hojarasca. Además, se registraron las especies vistas en encuentros ocasionales. Luego de un esfuerzo de 120 horas persona, se contabilizaron 19 especies representantes de 14 familias, 8 de reptiles y 6 de anfibios. La mayoría de las especies fueron registradas durante los muestreos (63 %, n= 12); solamente una especie, el sapo Incilius coccifer, fue registrada tanto en los muestreos como en encuentros ocasionales. Seis de las especies están asociadas a cuerpos de agua y se conocen por tolerar cierto grado de contaminación, de ahí que no sorprende su presencia en el área de estudio. El Bosque Demostrativo de la Sede de Occidente, fue el sitio con la mayor riqueza de especies registradas, probablemente por ser el sitio con mayor variedad de microhábitats para la fauna del lugar.
Azithromycin – more than an antibiotic
Authors:   2nd Department of Paediatrics, Centre of Postgraduate Medical Education, Warsaw, Poland, Sybilski Adam J. ,   Department of Paediatrics and Neonatology, Central Clinical Hospital of the Ministry of the Interior, Warsaw, Poland
Published: December 2020

Azithromycin, which belongs to the azalide subclass, is a derivative of erythromycin, obtained by inserting a methyl substituted nitrogen atom into the lactone ring. In this way, better stability at the low pH of the gastric juice, improved bioavailability, and an extended half-life relative to erythromycin were achieved. The mechanism of azithromycin antibacterial activity is to inhibit bacterial cell protein synthesis by binding to the 50S ribosomal subunit and preventing bacterial mRNA translation and protein biosynthesis. In addition to bactericidal activity, azithromycin also has immunomodulatory, anti-inflammatory, and antiviral properties. For this reason, it is used in exacerbations of asthma and chronic obstructive pulmonary disease. However, its antibacterial efficacy and high safety are most important. In clinical practice, several model clinical profiles of patients can be presented: children with community-acquired Mycoplasma pneumoniae pneumonia, recurrent respiratory tract infections and exacerbations of chronic inflammations, acute bronchitis of likely bacterial aetiology, tonsillitis and allergy to β-lactam antibiotics, and pertussis. Efficacy has also been proven in primary skin and soft tissue infections as well as erysipelas. However, azithromycin may also have an unusual use, as confirmed by recent reports. These include travellers’ diarrhoea, cat scratch disease (bartonellosis – rickettsiosis), and mycobacteriosis (nontuberculous mycobacteria). Appropriate spectrum of action, efficacy and safety, as well as a low percentage of resistant bacteria, good bioavailability, easy to remember, practical and simple dosage regimen and optimal cost are features of a good antibiotic. Azithromycin is close to meeting all these criteria.
Tactics of carrying out of auxiliary reproductive technologies at patients with accompanying adiposity and metabolic syndrome
Authors:   Shupyk National Medical Academy of Postgraduate Education, Kуiv, Ukraine, Petropavlovskay V.O.
Published: December 2020

The objective: restoration of reproductive function at women with accompanying adiposity and metabolic syndrome on the basis of studying at them clinical-functional and endocrinological features, and also improvement and introduction of algorithm of diagnostic and treatment-and-prophylactic actions with use of auxiliary reproductive technologies. Patients and methods. For studying of features of an induction superovulation at patients with superfluous weight of body and adiposity the retrospective analysis of treatment by methods of auxiliary reproductive technologies of 30 patients with IMB > 25kg/m2 (38 cycles of stimulation) has been carried out. Criteria of inclusion were age of 18–38 years; barreness that was the indication to ECO/IKSI; absence contra-indications to an induction superovulation and pregnancy. The comparison group was made by 30 patients at the age of 18–38 years without endocrinological infringements and in normal weight of a body (IМB – 18.5–24.9 kg/m2), indications to application ARТ for them were tube-peritoneale barreness owing to impassability or absence uterine pipes or the man’s factor of barreness. Results. Results of the spent researches testify about presence of essential difficulties at performance ART at women with superfluous weight of body and an accompanying metabolic syndrome. The results received by us are important from the scientific and theoretical point of view and allow insufficient efficiency of treatment of barreness at these patients. Conclusions. Results of treatment of infringements of reproductive function at women with accompanying adiposity and a metabolic syndrome that allows to recommend from in practical public health services allow to improve introduction of the received results. Key words: auxiliary reproductive technologies, superfluous weight bodies, adiposity.
Identification and characterization of antibodies against human Hsp60 at pregnancy
Authors:   Institute of postgraduate education, Bogomolets National Medical University, Kiev, Ukraine, Makarenko M. , Govsieiev D. ,   Institute of postgraduate education, Bogomolets National Medical University, Kiev, Ukraine, Vorona R. ,   Institute of postgraduate education, Bogomolets National Medical University, Kiev, Ukraine, Tsisarenko A. ,   Institute of Molecular Biology and Genetics of NAS of Ukraine, Kyiv, Vasil’tchuk O. ,   Institute of Molecular Biology and Genetics of NAS of Ukraine, Kyiv, Pavlyuk O. ,   Institute of Molecular Biology and Genetics of NAS of Ukraine, Kyiv, Yakovenko L. ,   Institute of Molecular Biology and Genetics of NAS of Ukraine, Kyiv, Kroupska I. ,   Institute of Molecular Biology and Genetics of NAS of Ukraine, Kyiv, Pogribna A. ,   Institute of Molecular Biology and Genetics of NAS of Ukraine, Kyiv, Sidorik L. ,   Institute of Molecular Biology and Genetics of NAS of Ukraine, Kyiv
Published: December 2020

Heat shock proteins (HSPs) or molecular chaperones were originally described as members of the cell response to different types of stress (infection, the influence of heavy metals, ischemia, hypoxia, amino acid starvation, psycho-emotional stress, hormonal stress, etc.). Hsp60 is one of the first proteins that are synthesized during embryogenesis. Hsp60 is necessary for the development of the embryo. The objective: was the identification and characterization of anti-Hsp60 autoantibodies in pregnant women to assess the prospects for a possible use as additional capabilities for the prediction of pregnancy progress. Patients and methods. Was a group of 170 pregnant women. 20 pregnant women were clinically healthy (control group) and 150 pregnant women had complicated anamnesis (main group). The level of IgG anti-Hsp60 antibodies in the serum was determined using immunoassay (ІFA). Immunoreactivity was determined by Western blot (immunoblotting). Results. Using the ІFA method, anti-Hsp60 positive serum was detected in 7.7% of pregnant women from the control group and in 14.0% of pregnant with complicated anamnesis. A statistically significant difference between the level of anti-Hsp60 autoantibody (on average) in pregnant women of the main group and the control group was not identified (0.177±0.118 vs. 0.134±0.097 units of optical density, p>0.05). Also, it was determined the frequency of detection of pathologies in pregnant women which have an anti-Hsp60 positive serum, compared with pregnant women which have an anti-Hsp60 negative serum. Conclusions. Our results suggest that in relation to the anti-Hsp60 antibody studies as an additional test, in particular, the forecast of progress of pregnancy, joint use ІFA and Western blotting is more appropriate. Key words: pregnancy, Hsp60, anti-Hsp60 autoantibodies.
“Love, Divine and Human: Contemporary Essays in Systematic and Philosophical Theology” edited by Oliver D. Crisp, James M. Arcadi and Jordan Wessling
Authors: Bray Dennis P.
Published: December 2020

Review of

Oliver D. Crisp, James M. Arcadi and Jordan Wessling, eds., Love, Divine and Human: Contemporary Essays in Systematic and Philosophical Theology (London: T&T Clark, 2020), pp. viii + 260, ISBN 978-0567687739. £95.00
On Issue of Civil and Legal Liability of the Insolvency Officer for Actions (Inaction) of Third Parties in a Bankruptcy Case
Authors: Staritsyn Igor
Published: December 2020

Considering the right of the insolvency officer to involve other persons, including employees of the debtor, to ensure his powers, the article examines the possibility of bringing the officer to civil and legal liability in the form of recovering damages for actions (inaction) of such persons. It summarizes and analyzes the judicial practice at the level of the Arbitration District Courts and the Judicial Panel on Economic Disputes of the Supreme Court of the Russian Federation. A conclusion is made of admissibility in certain cases of bringing the insolvency officer to civil and legal liability for the actions of the persons involved by him. The illegal behavior and the fault of the insolvency officer can be expressed in improper control over the actions (inactions) of third parties, in selecting an incompetent person. The article offers to regulate at the legislative level the issue of the possibility of bringing the insolvency officer to liability for actions of other persons, by including such rules in the Bankruptcy Law.
On Issue of the Concept Other Control Measures
Authors: Komarova Galina , Zmanovskaya Olga
Published: December 2020

The article examines approaches to defining the concept other control measures carried out by tax authorities. The opportunities of making such arrangements are envisaged in the RF Tax Code. The tax authorities powers in carrying out other control measures in the Code are not specified together with the fact that not specified are the content and consequences of these measures, except the responsibility for non-performance of the requirements. In practice, the tax authorities carry out other control measures in the process of pre-testing analysis in selecting taxpayers for the on-site tax audit. Carrying out other control measures increases the possibilities of monitoring legal compliance in taxes, levies and insurance contributions of the tax authorities but creates certain inconveniences and additional costs, problems with contractors for taxpayers. The contractors are requested to present the documents about the taxpayer, while the requirements do not always specify the transactions to be controlled, instead they indicate the number of the contract or the tax period.
Current State and Main Trends of Foreign Trade Development in Siberian Federal District
Authors: Sukhodolov Yakov , Kolodin Viktor
Published: December 2020

The article considers the current specificity of foreign trade ties of the Siberian Federal District. It presents a brief analysis of dynamics for the volumes and commodity structure of export-import operations of the Siberian Federal District based on an analysis of the statistical data of the Federal Customs Service of the Russian Federation during the period of 2015-2018. In currents terms and in view of forecasting of the world economy development, of special significance for the Siberian Federal District is the search for directions and tools of modernizing the economy and transition to the innovative development. The foreign trade can be a prospective direction of socio-economic development of the Siberian Federal District. At the present time, the Siberian Federal District is export-oriented, but in the commodity structure of the export there prevail raw mineral groups of commodities, which decrease the positive effect from the export. Developing processing industries and machine-building oriented at increasing the depth of processing the raw resources and the finished goods export can be the basis of the sustainable development of Siberia.
SUSTAINABILITY OF PEDOBIONT MEADOW COMMUNITIES UNDER ANTHROPOGENIC LOAD
Authors:   Institute for Ecology and Subsoil Use of the Academy of Sciences of the Republic of Tatarstan (Kazan, Russia), Gordienko T.A. , Sukhodolskaya R.A. ,   Institute for Ecology and Subsoil Use of the Academy of Sciences of the Republic of Tatarstan (Kazan, Russia), Vavilov D.N. ,   Institute for Ecology and Subsoil Use of the Academy of Sciences of the Republic of Tatarstan (Kazan, Russia), Lukyanova Yu.A. ,   Nizhnyaya Kama National Park (Elabuga, Russia)
Published: December 2020

Studies of the soil mesofauna were carried out in Tanaev meadows of the «Nizhnyaya Kama» National Park in natural and disturbed areas. The abundance of pedobionts and herpetobionts in natural areas significantly exceeded the abundance in disturbed areas. However, the taxonomic composition and trophic structure were similar. In general, communities structure indexes in natural sites indicate a stable ecosystem of floodplain meadows. The observance of pipe laying technology during further restoration of disturbed areas, high soil moisture and a small width of the disturbed soil layer contribute to the preservation and rapid restoration of the structure and population of large soil and litter invertebrates of meadow phytocenosis.
STUDIES OF CARABIDS COMPLEXES INSIDE THE CITY BLOCKS IN KEMEROVO
Authors:   Kemerovo State University (Kemerovo, Russia), Eremeeva N.I.
Published: December 2020

Studies of carabids complexes inside the city blocks on the example of one district of the city were conducted. Carabids were studied on lawns, in coniferous and deciduous plantings. 46 species of ground beetles were found, including 38 species on lawns and 28 species in plantings. On the lawns are dominated by carabids Poecilus versicolor, Amara aenea, Poecilus fortipes, Harpalus rufipes. In the coniferous and deciduous plantations are dominated by the species Harpalus affinis, Pterostihus niger, Pterostihus melanarius and Harpalus rufipes.
TAXONOMIC STRUCTURE OF NIDICOLE FAUNA IN THE NESTS OF THE SAND MARTIN (RIPARIA RIPARIA LINNAEUS, 1758) IN THE TERRITORY OF THE SARATOV REGION
Authors:   Saratov State University N. G. Chernyshevsky (Saratov, Russia), Kondratyev E.N.
Published: December 2020

Today, global climate changes are taking place, leading to changes in the habitats of many species, including organisms of epidemiological importance. The transfer of such organisms will primarily involve the blood-sucking parasites of migratory birds. The sand martin (Riparia riparia Linnaeus, 1758) is one of many migratory birds nesting in the Saratov region. In order to understand how much the species is involved in the transmission of infection and the creation of a new focal point of infection, it is necessary to establish the taxonomic structure of the nidicol fauna.
THE USE OF PHEROMONE TRAPS IN TRAINING FIELD PRACTICE
Authors:   Petrozavodsk State University (Petrozavodsk, Russia), Lyabzina S.N. ,   All-Russian Plant Quarantine Centre (Petrozavodsk, Russia), Zinnikov D.F. ,   All-Russian Plant Quarantine Centre (Petrozavodsk, Russia)
Published: December 2020

In field practice on invertebrate zoology and other subjects, in addition to the traditional methods of collecting insects, we can use pheromone traps. Traps were applied in studying the dynamics of xylophilous insects: a sawyer beetle and two bark beetles (an eight-dentated bark beetle and a six-dentated bark beetle). The pheromonitoring have been obtained seasonal variations in the number of populations and density of these species of some nature conservation area in Karelia. The collection efficiency of bark beetles during the period of the maximum number of species was about 600 individuals.
SEASONAL DYNAMICS OF CICADAS (HEMIPTERA: CICADINA) ON THE TERRITORY OF THE TIGIREKSKY NATURE RESERVE (ALTAI KRAI)
Authors:   Lobachevsky State University (Nizhny Novgorod, Russia), Mayorova E.Yu.
Published: December 2020

The seasonal dynamics of cicadas (Hemiptera: Cicadina) has been investigated at five model locations of Tigireksky Nature Reserve (Altai Krai). 65 cicada species of 43 genera and 5 families have been identified. Two association clusters have been built: aestival and late-summer/autumnal. The maximum abundance for the families Cicadellidae and Membracidae was observed during latesummer/ autumnal period (early September and late July, respectively).
DISTRIBUTION OF HORTHOBIONIC INSECTS (HETEROPTERA) ON THE MEADOW STATIONS IN THE TERRITORY OF THE RYAZAN REGION
Authors:   Oka State Natural Biosphere Reserve (Ryazan Region, Brykin Bor village, Russia), Nikolaeva A.M.
Published: December 2020

378 species of hemiptera insects are known now for the Ryazan region. The article discusses changes in the complexes of hemiptera insects in meadow habitats. We present data for 3 species new to the territory.
REVISION OF FAUNA BUMBLEBEE (HYMENOPTERA: APIDAE) OF ARCHIPELAG NEW EARTH
Authors:   FCIARCTIC UB RAS (Arkhangelsk, Russia), Potapov G.S. , Kolosova Yu.S. ,   FCIARCTIC UB RAS (Arkhangelsk, Russia)
Published: December 2020

Fauna of invertebrates of the Novaya Zemlya Archipelago is rather poorly known, while a growing body of recent molecular research indicates that several Arctic species may represent endemic lineages with restricted ranges. These local endemics need special conservation efforts because of the increasing anthropogenic pressure and climate changes. Here, we re-examine the bumblebee fauna of the Novaya Zemlya Archipelago by using the phylogeographic analysis.
SEXUAL DIMORPHISM OF THE PTEROSTICHUS MELANARIUS ILL. (COLEOPTERA, CARABIDAE) AND THE CONDITIONS OF ITS MANIFESTATION
Authors:   Institute for Ecology and Subsoil Use of the Academy of Sciences of the Republic of Tatarstan (Kazan, Russia), Sukhodolskaya R.A. , Timofeeva G.A. ,   Bogorodskaya secondary school of Pestrechinsky district of the Republic of Tatarstan (Russia), Ukhova N.L. ,   Visimsky state natural biosphere reserve (Kirovgrad, Russia), Vorobieva I.G. ,   Maria State University (Yoshkar-Ola, Republic of Mari El, Russia), Saveliev A.A. ,   Kazan (Povolzhsky) Federal University (Kazan, Russia)
Published: December 2020

This study presents materials on the variability of SD in Carabidae, depending on habitats - open, shaded, shrubs, city, suburb.
MONITORING OF TICK-BORNE INFECTIONS (IXODIDAE) IN THE TOMSK REGION
Authors:   Federal Public Health Institution ", Poltoratskaya N.V. ,   Center of Hygiene and Epidemiology in Tomsk Region",   (Tomsk, Russia),   Tomsk State University (Tomsk, Russia), Poltoratskaya T.N. ,   Federal Public Health Institution "Center of Hygiene and Epidemiology in Tomsk Region" (Tomsk, Russia), Pankina T.M. ,   Federal Public Health Institution "Center of Hygiene and Epidemiology in Tomsk Region" (Tomsk, Russia), Shikhin A.V. ,   Federal Public Health Institution "Center of Hygiene and Epidemiology in Tomsk Region" (Tomsk, Russia)
Published: December 2020

The analysis of epidemiological monitoring for the period 2017–2019 for infections transmitted by ixode ticks in Tomsk region is presented. The incidence of ixodic tick-borne borreliosis (ICB) is twice as high as that of tick-borne encephalitis (CE). The main morbidity (over 82%) falls on the southern regions of the Tomsk Oblast. Most infections occurred at the place of residence of the victims.
FISH INFECTIONS BY TREMATODES OF THE GENUS POSTHODIPLOSTOMUM IN THE SOUTHERN REGIONS OF THE TOMSK REGION
Authors:   ISEA SB RAS (Novosibirsk, Russia), Serbina E.A. ,   Siberian State University of Telecommunications and Informatics (Novosibirsk), Interesova E.A. ,   ISEA SB RAS (Novosibirsk, Russia); Novosibirsk branch of VNIRO (Novosibirsk, Russia)
Published: December 2020

Infection of the fish with trematode metacercariae of the genus Posthodiplostomum in rivers in the southern regions of the Tomsk region are presented. In this study it was shown that the abundance trematode metacercariae of the genus Posthodiplostomum is statistically significantly higher in fish in the Ob river in comparison with the Tom river. The assumption is put forward that the appearance of this parasite on the territory of the Tomsk Region is associated with the expansion to the north of the area of the gray heron Area cinereal, which is the final host of trematodes of the genus Posthodiplostomum.
VETERINARY AND MEDICAL SIGNIFICANCE OF GADFLIES (DIPTERA, TABANIDAE)
Authors:   All-Russian Research Institute of Veterinary Entomology and Arachnology (Tyumen, Russia), Sivkova E.I.
Published: December 2020

Now the interest of scientists and researchers in dipterous insects considerably increased. Blood-sicking dipterous insects (midges), include in the structure of mosquitoes (Culicidae), gadflies (Tabanidae), midges (Simuliidae) and other insects. The major factors determining the high number of midges are favorable climatic conditions for their reproduction and existence in combination with abundance of biotopes of cultivation (various reservoirs and marsh educations) and dwellings of an imago (existence of wood, shrubby or high grassy vegetation) and also presence of enough warm-blooded animals – a saturation source blood. The harm done by gadflies to the person and an animal is very big.
INFLUENCE OF INSECTICIDE BREEDING ON BIOLOGICAL PARAMETERS OF MUSCA DOMESTICA L. (DIPTERA: MUSCIDAE)
Authors:   All-Russian Research Institute of Veterinary Entomology and Arachnology (Tyumen, Russia), Silivanova E.A. , Shumilova P.A. ,   All-Russian Research Institute of Veterinary Entomology and Arachnology (Tyumen, Russia), Levchenko M.A. ,   All-Russian Research Institute of Veterinary Entomology and Arachnology (Tyumen, Russia)
Published: December 2020

The goal of the current research was to evaluate the biological parameters of insects when they were exposed to insecticides for several generations. In the experiments, the adults of the house fly Musca domestica L. were feed with one of two insecticides (chlorfenapyr or fipronil) in each generation. The duration of development stages, fecundity, the weight of larvae, pupa, and adults, as well as the sizes of females and males, were evaluated. The statistical significance of differences in biological parameters was assessed by the non-parametric Kruskal-Wallis and Dunn criteria. The duration of the larva stages lasted 2.2 times more in the fourth generation of chlorfenapyr- and fipronil-exposure strains compared to the control laboratory strain of M. domestica. Increasing the period of preimaginal stages of the insect life cycle can be considered as a sublethal effect of insecticides.
RANGE OF DIAGNOSTIC CONCENTRATIONS FROM DIFFERENT GROUPS OF INSECTICIDES IN RELATION TO INVASIVE SPECIES OF MOSQUITOES OF THE GENUS AEDES
Authors:   Research Institute of Disinfectology of Rospotrebnadzor (Moscow, Russia), Frolova A.I.
Published: December 2020

Data on the susceptibility to insecticides of various structures in mosquito larvae of Aedes albopictus that is a vector of arboviral fevers are given. Diagnostic concentrations of insecticides for the detection of insecticide resistance in Ae. albopictus populations from different districts of the Russia Black Sea coast of the Caucasus are calculated.
Desenvolvimento de Curvas Intensidade-Duração-Frequência para Dois Municípios do Sertão de Pernambuco
Authors: Santana Natalia Fernanda Jeronimo de , Silva Junior Marcos Antonio Barbosa da
Published: December 2020

Para o dimensionamento e projeto das obras hidráulicas, faz-se necessário conhecer/obter a equação de chuvas intensas, que relaciona três grandezas características das precipitações máximas: intensidade, duração e frequência. Neste sentido, o presente trabalho determina as equações IDFs (Intensidade-Duração-Frequência) para os municípios de Itapetim e Brejinho, ambos situados no sertão de Pernambuco. Para isso, foram utilizadas as séries históricas diárias da Agência Pernambucana de Águas e Clima (APAC) dos postos de Brejinho (cód. 437) e Itapetim (cód. 151), com dados de 20 anos (2000-2019) e 27 anos (1993-2019), respectivamente. Os dados foram consistidos utilizando o método da Dupla Massa e as alturas de chuvas máximas diárias, determinadas para diferentes tempos de retorno, foram obtidas através do método de distribuição estatística de Gumbel e, posteriormente, desagregadas para durações entre 24 horas e 6 minutos. Por meio de planilhas eletrônicas do Microsoft Excel, foram obtidas as curvas IDFs para os tempos de retorno de 5, 10, 15, 25, 50, 100, 500, 1.000 e 10.000 anos, bem como suas relações matemáticas. Para Brejinho, a equação da família de curvas IDF obtida foi i = 1318,06Tr0,19 (t + 11,5)-0,759 e, para Itapetim, a equação foi i = 1028,68Tr0,19 (t + 11,51)-0,759, em que “i” é a intensidade máxima média (mm h-1), “t” a duração (minuto) e “Tr” o período de retorno (ano) das chuvas. A obtenção das citadas equações de chuvas intensas poderá contribuir para futuros estudos hidrológicos nos municípios.
Triagem Paulista: inventário e diretrizes para preservação da memória do conjunto ferroviário arquitetônico em Bauru (SP)
Authors: Almeida Natasha Neme Gonçalves de , Gomes Samir Hernandes Tenório
Published: December 2020

As discussões de metodologias que vinculem a preservação de conjuntos ferroviários a novos usos na dinâmica urbana vêm ganhando espaço crescente dentro da arqueologia industrial atualmente. No oeste paulista, o desenvolvimento da malha ferroviária impulsionou o crescimento de diversas cidades e formou um patrimônio cultural material e imaterial diversificado. A história desse patrimônio está diretamente vinculada a grupos sociais, conjuntos ferroviários, deslocamentos humanos, assentamentos e infraestruturas urbanas inteiras que se transformaram ao longo do tempo. Esse estudo se insere nesse contexto e visa reconstituir memória do conjunto arquitetônico presente no antigo complexo ferroviário de Triagem, localizado em Bauru (SP), o qual foi palco estratégico da logística e manutenção dos equipamentos rodantes da Companhia Paulista de Estradas de Ferro e hoje encontra-se obsoleto, com uma série de conflitos com seu entorno. Mesmo nessa conjuntura, a área apresenta vestígios de usos por alguns grupos sociais e partilha relações de afetividade no imaginário coletivo da população remetendo a seu período operacional. O objetivo dessa pesquisa é inventariar a memória do conjunto ferroviário de Triagem Paulista e, por meio de análise comparativa das formas de apropriação do objeto em seu período operacional e atual, propor metodologias e diretrizes para preservação das suas edificações mais significativas. Para tanto, a metodologia envolveu a pesquisa documental e bibliográfica, entrevistas, mapeamento e pesquisa de campo a fim de relacionar os contextos antigo e atual do conjunto e reinseri-lo no dinâmica atual da cidade.
Sistemas de Informação Geográfica (SIG’s) na análise do entorno de aterros sanitários e a Democratização / Inclusão Geotecnológica
Authors: Bezerra João Paulo Peres , Leal Antonio Cezar , Nunes Rafael Silva
Published: December 2020

A elevada geração de resíduos sólidos é uma problemática crescente e faz parte dos males que incidem na crise ambiental vivenciada nos tempos atuais em todo o mundo, demandando estratégias e ferramentas de gestão de resíduos sólidos urbanos. O presente texto tem como objetivo realizar uma reflexão teórica sobre geotecnologias, sistemas de informação geográfica e geoprocessamento aplicados à gestão de resíduos sólidos urbanos. Especificamente, traz para o debate uma revisão dos fundamentos teóricos referente à geotecnologia e propõe avanços na democratização de dados e informações geoespaciais, com o escopo da democratização geotecnológica. Ainda, o texto apresenta dois estudos de análise espacial realizados por meio de procedimentos de análise de proximidade e vizinhança no entorno de locais de disposição final de resíduos sólidos, tendo como base as especificidades de normas técnicas, no que tange a proximidade destas áreas com corpos hídricos e núcleos habitacionais.
Differentiation of hPSCs to hypothalamic neurons v1
Authors: Chen Cortina , Mali Iman , T Merkle Florian
Published: December 2020

This protocol is about Differentiation of hPSCs to hypothalamic neurons.
Preparing hPSC-derived neurons for single-cell RNA sequencing v1
Authors: Chen Cortina , T Merkle Florian
Published: December 2020

This protocol is about Preparing hPSC-derived neurons for single-cell RNA sequencing.
Metabarcoding MinION library prep using custom primers v2
Authors: Regan Tim
Published: December 2020

This protocol follows from dx.doi.org/10.17504/protocols.io.bms8k6hw; i.e. starting with marine sample derived DNA extracts. It describes a protocol to prepare 16S and 18S metabarcoding libraries for microbial population analysis using Oxford Nanopore Technology Flow cell sequencing.
COVID-19 Pandemic: A Challenge for Healthcare Professionals and Assessment of Anxiety Symptoms
Authors: Maestro Daniel , Šegalo Sabina , Remić Dinko , Pašalić Arzija , Jogunčić Anes
Published: December 2020

Introduction: Worldwide, COVID-19 pandemic caused millions of infected people and thousands of deaths. Due to enormous pressure on health-care systems and its inadequate preparedness, utter collapse is expected. In the current epidemic response, healthcare workers’ (HCWs) knowledge and practice are crucial, while the impact on their mental health is still unknown.Methods: The cross-sectional study was conducted among HCWs redeployed to COVID points in the Public Institution Health Centre of Sarajevo Canton. According to guidelines and information provided by the World Health Organization and Ministries of Health, a questionnaire was developed. In addition, General Anxiety Disorder-7 as a screening tool for anxiety disorders was used.Results: Of 180 respondents, 26 (14.4%) were in direct contact with the sick patient. In total, 79 (43.9%) respondents consider their personal protective equipment is in accordance with the guidelines of the world health authorities. A total of 72 (39.7%) of respondents used the same mask for several days. In general, the danger from new coronavirus was considered minimal by 59 (32,6%) HCWs. Based on the achieved score for assessing the anxiety disorder, in 63 (35%) subjects, the presence of severe symptoms was detected.Conclusion: This study found that most HCWs do not have enough knowledge about the COVID-19 pandemic. We identified that there are differences in the sources of information and gap in perceptions of the native origin of the virus. Considering the frequency of anxiety symptoms among HCWs, interventions are necessary in order to preserve their mental health.
Computed tomography simulator conversion curve dependence on scan parameters and phantom dimension
Authors: Kolarevic Goran , Jaroš Dražan , Pavičar Bojan , Ignjć Tatjana , Kostovski Aleksandar , Marosevic Goran , Predojević Branko , Mirjanić Dragoljub
Published: December 2020

Introduction: Using computed tomography (CT) and treatment planning systems (TPS) in radiotherapy, due to the difference in photon beam energy on CT and linear accelerator, it is necessary to convert Hounsfield units (HU) to relative electron density (RED) values. The aim of this dosimetric study was to determine whether there is a significant effect of potential in the CT tube, field of view size (FOV), and phantom dimensions on the CT conversion curve CT-RED. The second aim is whether there are significant differences between the CT-RED obtained by the Computerized Imaging Reference Systems (CIRS) Thorax 002LFC phantom and the “reference” curve in the TPS, obtained by the CIRS 062M pelvis phantom, at the same CT conditions.Methods: Heterogeneous CIRS 062M and CIRS Thorax 002LFC phantoms were used, which anatomically and dimensionally represent the human pelvis, head, and thorax, with a set of known RED inserts. They were scanned on a CT LightSpeed GE simulator and obtained CT-RED.Results: The high voltage in the CT tube had a significant effect on the HU (t = 10.72, p < 0.001) for RED values >1.1, while FOV as a parameter did not show statistical significance for the 062M pelvis phantom. Comparing the slopes (062M pelvis and head) of the CT-RED for RED ≥ 1.1, the obtained value is t = 1.404 (p = 0.163). In the case of a 062M pelvis and a 002LFC phantom, we have seen a difference in RED values (for the same HU value) of 5 % in the RED region ≥ 1.1 (bone).Conclusion: Patients should be imaged on a CT simulator only at the potential of the CT tube on which the conversion curve was recorded. The influence of the FOV and scanned phantom dimensions is not statistically significant on the appearance of the calibration curve (RED ≥ 1.1).