bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of herbivorous gastropod grazing on the sedimentation and succession of subtidal macroalgal assemblages
Authors: Suzuki H , Kubo Y , Inomata E , Agatsuma Y , Aoki MN
Published: November 2020

The subtidal zone on cold temperate rocky coasts is an environment exposed to much less physical disturbance than the intertidal, and sediment deposition is continuous. Removal of this sediment by gastropod grazers is therefore presumed to affect the structure of subtidal algal communities. This concept was investigated by evaluating the grazing effects of the dominant herbivorous gastropod Omphalius rusticus by exclusion experiments in the field. Settlement plates of both exclusion and control treatments were placed every month from November 2014, and immersed for 1 mo. Algae colonized from May and tended to increase in biomass toward summer. No marked differences were observed in the algal composition of exclusion and control. Sediment deposition showed no apparent seasonal changes. Cumulative successional plates of both exclusion and control treatments were placed in November 2014, and immersed for 1 to 9 mo. The colonization of algae started in February and the species number peaked earlier in the exclusion and later in the control. Sediment load and algal biomass were high from February to May in the exclusion, and from April to July in the control. Seedlings of Sargassum confusum were found in both plots starting in July. We conclude that O. rusticus constantly removed sediments by its grazing activity and had a large impact on the formation of the macroalgal community by controlling sediment deposition. Its presence delayed the colonization of early-successional turf algal species, but did not affect colonization of late-successional canopy-forming algae such as S. confusum.
NEWS & VIEWS
Authors: Masoodi Shariq R
Published: November 2020

The World Health Organisation (WHO): https://www.who.int/emergencies/diseases/novel-coronavirus-2019

The Centers for Disease Control and Prevention (CDC): https://www.coronavirus.gov.

The National Institutes of Health (NIH): https://www.nih.gov/coronavirus.

The National Center for Biotechnology Information (NCBI): https://www.ncbi.nlm.nih.gov/sars-cov-2/.

The New England Journal of Medicine (NEJM): https://www.nejm.org/coronavirus

The Lancet: https://www.thelancet.com/coronavirus

British Medical Journal (BMJ): https://www.bmj.com/coronavirus

Journal of American Medical Association (JAMA): https://jamanetwork.com/journals/jama/pages/coronavirus-alert

Royal College for Physicians (Edinburgh): https://www.rcpe.ac.uk/covid-19-hub

American College for Physicians (ACP): https://www.acponline.org/clinical-information/clinical-resources-products/coronavirus-disease-2019-covid-19-information-for-internists

UpToDate: https://www.uptodate.com/home/covid-19-access

Indian Council of Medical Research (ICMR): https://www.icmr.gov.in/
Anthropogenic Influences on 2019 July Precipitation Extremes Over the Mid–Lower Reaches of the Yangtze River
Authors: Nanding Nergui , Chen Yang , Wu Huan , Dong Buwen , Tian Fangxing , Lott Fraser C. , Tett Simon F. B. , Rico-Ramirez Miguel Angel , Chen Yiheng , Huang Zhijun , Yan Yan , Li Delei , Li Rouke , Wang Xuan , Fan Xuewei
Published: November 2020

Understanding the driving factors for precipitation extremes matters for adaptation and mitigation measures against the changing hydrometeorological hazards in Yangtze River basin, a habitable area that provides water resources for domestic, farming, and industrial needs. However, the region is naturally subject to major floods linked to monsoonal heavy precipitation during May–September. This study aims to quantify anthropogenic influences on the changing risk of 2-week-long precipitation extremes such as the July 2019 extreme cases, as well as events of shorter durations, over the middle and lower reaches of Yangtze River basin (MLYRB). Precipitation extremes with different durations ranging from 1-day to 14-days maximum precipitation accumulations are investigated. Gridded daily precipitations based on nearly 2,400 meteorological stations across China are used to define maximum accumulated precipitation extremes over the MLYRB in July during 1961–2019. Attribution analysis is conducted by using the Met Office HadGEM3-GA6 modeling system, which comprises two sets of 525-member ensembles for 2019. One is forced with observed sea-surface temperatures (SSTs), sea-ice and all forcings, and the other is forced with preindustrialized SSTs and natural forcings only. The risk ratio between the exceedance probabilities estimated from all-forcing and natural-forcing simulations is calculated to quantify the anthropogenic contribution to the changing risks of the July 2019–like precipitation extremes. The results reveal that anthropogenic warming has reduced the likelihood of 2019-like 14-days heavy precipitation over the mid–lower reaches of the Yangtze River by 20%, but increased that of 2-days extremes by 30%.
Platelets Selectively Regulate the Release of BDNF, But Not That of Its Precursor Protein, proBDNF
Authors: Le Blanc Jessica , Fleury Samuel , Boukhatem Imane , Bélanger Jean-Christophe , Welman Mélanie , Lordkipanidzé Marie
Published: November 2020

Background

Brain-derived neurotrophic factor (BDNF) plays a role in synaptic plasticity and neuroprotection. BDNF has well-established pro-survival effects, whereas its precursor protein, proBDNF, induces apoptosis. Thus, it has been suggested that the proBDNF/BDNF ratio could be an indicator of neuronal health. Access to neurons is, understandably, limited. Because of their similarities, platelets have been put forward as a non-invasive biomarker of neuronal health; indeed, they store large quantities of BDNF and can release it into circulation upon activation, similarly to neurons. However, whether platelets also express the precursor proBDNF protein remains unknown. We therefore sought to characterize proBDNF levels in human platelets and plasma.

Methods

The presence of proBDNF was assessed by immunoblotting, cell fractionation, flow cytometry, and confocal microscopy in washed platelets from 10 healthy volunteers. Platelets from 20 independent healthy volunteers were activated with several classical agonists and the release of BDNF and proBDNF into plasma was quantified by ELISA.

Results

Platelets expressed detectable levels of proBDNF (21 ± 13 fmol/250 x 106 platelets). ProBDNF expression was mainly localized in the intracellular compartment. The proBDNF to BDNF molar ratio was ~1:5 in platelets and 10:1 in plasma. In stark contrast to the release of BDNF during platelet activation, intraplatelet and plasma concentrations of proBDNF remained stable following stimulation with classical platelet agonists, consistent with non-granular expression.

Conclusions

Platelets express both the mature and the precursor form of BDNF. Whether the intraplatelet proBDNF to BDNF ratio could be used as a non-invasive biomarker of cognitive health warrants further investigation.
The Role of Systems Vaccinology in Understanding the Immune Defects to Vaccination in Solid Organ Transplant Recipients
Authors: Scanlon Nicholas , Saklawi Youssef , Rouphael Nadine
Published: November 2020

Solid organ transplant recipients (SOTRs) are at increased risk for many infections, whether viral, bacterial, or fungal, due to immunosuppressive therapy to prevent organ rejection. The same immune defects that render transplanted patients susceptible to infection dampen their immune response to vaccination. Therefore, it is vital to identify immune defects to vaccination in transplant recipients and methods to obviate them. These methods can include alternative vaccine composition, dosage, adjuvants, route of administration, timing, and re-vaccination strategies. Systems biology is a relatively new field of study, which utilizes high throughput means to better understand biological systems and predict outcomes. Systems biology approaches have been used to help obtain a global picture of immune responses to infections and vaccination (i.e. systems vaccinology), but little work has been done to use systems biology to improve vaccine efficacy in immunocompromised patients, particularly SOTRs, thus far. Systems vaccinology approaches may hold key insights to vaccination in this vulnerable population.
Regulation of PD-L1 Expression by NF-κB in Cancer
Authors: Antonangeli Fabrizio , Natalini Ambra , Garassino Marina Chiara , Sica Antonio , Santoni Angela , Di Rosa Francesca
Published: November 2020

Immune checkpoints are inhibitory receptor/ligand pairs regulating immunity that are exploited as key targets of anti-cancer therapy. Although the PD-1/PD-L1 pair is one of the most studied immune checkpoints, several aspects of its biology remain to be clarified. It has been established that PD-1 is an inhibitory receptor up-regulated by activated T, B, and NK lymphocytes and that its ligand PD-L1 mediates a negative feedback of lymphocyte activation, contributing to the restoration of the steady state condition after acute immune responses. This loop might become detrimental in the presence of either a chronic infection or a growing tumor. PD-L1 expression in tumors is currently used as a biomarker to orient therapeutic decisions; nevertheless, our knowledge about the regulation of PD-L1 expression is limited. The present review discusses how NF-κB, a master transcription factor of inflammation and immunity, is emerging as a key positive regulator of PD-L1 expression in cancer. NF-κB directly induces PD-L1 gene transcription by binding to its promoter, and it can also regulate PD-L1 post-transcriptionally through indirect pathways. These processes, which under conditions of cellular stress and acute inflammation drive tissue homeostasis and promote tissue healing, are largely dysregulated in tumors. Up-regulation of PD-L1 in cancer cells is controlled via NF-κB downstream of several signals, including oncogene- and stress-induced pathways, inflammatory cytokines, and chemotherapeutic drugs. Notably, a shared signaling pathway in epithelial cancers induces both PD-L1 expression and epithelial–mesenchymal transition, suggesting that PD-L1 is part of the tissue remodeling program. Furthermore, PD-L1 expression by tumor infiltrating myeloid cells can contribute to the immune suppressive features of the tumor environment. A better understanding of the interplay between NF-κB signaling and PD-L1 expression is highly relevant to cancer biology and therapy.
Lipid Mediators in Critically Ill Patients: A Step Towards Precision Medicine
Authors: Cioccari Luca , Luethi Nora , Masoodi Mojgan
Published: November 2020

A dysregulated response to systemic inflammation is a common pathophysiological feature of most conditions encountered in the intensive care unit (ICU). Recent evidence indicates that a dysregulated inflammatory response is involved in the pathogenesis of various ICU-related disorders associated with high mortality, including sepsis, acute respiratory distress syndrome, cerebral and myocardial ischemia, and acute kidney injury. Moreover, persistent or non-resolving inflammation may lead to the syndrome of persistent critical illness, characterized by acquired immunosuppression, catabolism and poor long-term functional outcomes. Despite decades of research, management of many disorders in the ICU is mostly supportive, and current therapeutic strategies often do not take into account the heterogeneity of the patient population, underlying chronic conditions, nor the individual state of the immune response. Fatty acid-derived lipid mediators are recognized as key players in the generation and resolution of inflammation, and their signature provides specific information on patients’ inflammatory status and immune response. Lipidomics is increasingly recognized as a powerful tool to assess lipid metabolism and the interaction between metabolic changes and the immune system via profiling lipid mediators in clinical studies. Within the concept of precision medicine, understanding and characterizing the individual immune response may allow for better stratification of critically ill patients as well as identification of diagnostic and prognostic biomarkers. In this review, we provide an overview of the role of fatty acid-derived lipid mediators as endogenous regulators of the inflammatory, anti-inflammatory and pro-resolving response and future directions for use of clinical lipidomics to identify lipid mediators as diagnostic and prognostic markers in critical illness.
Pathogenic Factors Correlate With Antimicrobial Resistance Among Clinical Proteus mirabilis Strains
Authors: Filipiak Aneta , Chrapek Magdalena , Literacka Elżbieta , Wawszczak Monika , Głuszek Stanisław , Majchrzak Michał , Wróbel Grzegorz , Łysek-Gładysińska Małgorzata , Gniadkowski Marek , Adamus-Białek Wioletta
Published: November 2020

Proteus mirabilis is the third most common etiological factor of urinary tract infection. It produces urease, which contributes to the formation of a crystalline biofilm, considered to be one of the most important virulence factors of P. mirabilis strains, along with their ability to swarm on a solid surface. The aim of this study was to analyze the pathogenic properties of two selected groups of clinical P. mirabilis isolates, antimicrobial susceptible and multidrug resistant (MDR), collected from hospitals in different regions in Poland. The strains were examined based on virulence gene profiles, urease and hemolysin production, biofilm formation, and swarming properties. Additionally, the strains were characterized based on the Dienes test and antibiotic susceptibility patterns. It turned out that the MDR strains exhibited kinship more often than the susceptible ones. The strains which were able to form a stronger biofilm had broader antimicrobial resistance profiles. It was also found that the strongest swarming motility correlated with susceptibility to most antibiotics. The correlations described in this work encourage further investigation of the mechanisms of pathogenicity of P. mirabilis.
Human Papillomavirus Infections in Cervical Samples From HIV-Positive Women: Evaluation of the Presence of the Nonavalent HPV Genotypes and Genetic Diversity
Authors: Sias Catia , Guarrasi Valerio , Minosse Claudia , Lapa Daniele , Nonno Franca Del , Capobianchi Maria Rosaria , Garbuglia Anna Rosa , Del Porto Paola , Paci Paola
Published: November 2020

Non-nonavalent vaccine (9v) Human papillomavirus (HPV) types have been shown to have high prevalence among HIV-positive women. Here, 1444 cervical samples were tested for HPV DNA positivity. Co-infections of the 9v HPV types with other HPV types were evaluated. The HPV81L1andL2genes were used to investigate the genetic variability of antigenic epitopes. HPV-positive samples were genotyped using the HPVCLART2 assay. The L1 and L2 protein sequences were analyzed using a self-optimized prediction method to predict their secondary structure. Co-occurrence probabilities of the 9v HPV types were calculated. Non9v types represented 49% of the HPV infections; 31.2% of the non9v HPV types were among the low-grade squamous intraepithelial lesion samples, and 27.3% among the high-grade squamous intraepithelial lesion samples, and several genotypes were low risk. The co-occurrence of 9v HPV types with the other genotypes was not correlated with the filogenetic distance. HPV81 showed an amino-acid substitution within the BC loop (N75Q) and the FGb loop (T315N). In the L2 protein, all of the mutations were located outside antigenic sites. The weak cross-protection of the 9v types suggests the relevance of a sustainable and effective screening program, which should be implemented by HPV DNA testing that does not include only high-risk types.
Multicentric Retrospective Evaluation of Five Classic Infantile Pompe Disease Subjects Under Enzyme Replacement Therapy With Early Infratentorial Involvement
Authors: Paoletti Matteo , Pichiecchio Anna , Colafati Giovanna Stefania , Conte Giorgio , Deodato Federica , Gasperini Serena , Menni Francesca , Furlan Francesca , Rubert Laura , Triulzi Fabio Maria , Cinnante Claudia
Published: November 2020

White matter (WM) abnormalities and ventricular enlargement in brain MRI are well-known features in infantile-onset Pompe disease (IOPD) in the era of enzyme replacement therapy (ERT). In this multicentric observational retrospective study, we report a small cohort of IOPD subjects under ERT treatment (n = 5, median age at MRI = 7.4 years, median period of treatment = 85 months) that showed the classic features of extensive supratentorial WM abnormalities but also unusual findings such as early infratentorial WM abnormalities and late supratentorial U-fiber involvement. Given the recent implementation of ERT and the rarity of the disease, a complete spectrum of presentation and understanding of progressive pathology in the brain of IOPD subjects in treatment remains underacknowledged. The availability of long-term follow-up of IOPD subjects under ERT treatment allows a better insight into the evolution of brain abnormalities in such disease.
GJA1 Gene Polymorphisms and Topographic Distribution of Cranial MRI Lesions in Cerebral Small Vessel Disease
Authors: Zhang Jing , You Qian , Shu Junlong , Gang Qiang , Jin Haiqiang , Yu Meng , Sun Wei , Zhang Wei , Huang Yining
Published: November 2020

Vascular endothelial cell (EC) and blood–brain barrier (BBB) dysfunction is the core pathogenesis of cerebral small vessel disease (CSVD). Moreover, animal experiments have shown the importance of connexin (Cx)-43 in EC and BBB function. In this study, we recruited 200 patients diagnosed with sporadic CSVD. Initially, we examined imaging scores of white matter hyperintensities (WMH), lacunar infarction (LI), and cerebral microbleeds (CMB). Additionally, we performed next-generation sequencing of the GJA1 gene (Cx43 coding gene) to examine correlation between these single-nucleotide polymorphisms and the burden and distribution of CSVD. Fourteen target loci were chosen. Of these, 13 loci (92.9%) contributed toward risk for cerebellar LI, one locus (7.1%) was shown to be a protective factor for lobar CMB after FDR adjustment. In conclusion, single-nucleotide polymorphisms in the GJA1 gene appear to affect the distribution but not severity of CSVD.
Targeting the Ubiquitin System in Glioblastoma
Authors: Scholz Nico , Kurian Kathreena M. , Siebzehnrubl Florian A. , Licchesi Julien D. F.
Published: November 2020

Glioblastoma is the most common primary brain tumor in adults with poor overall outcome and 5-year survival of less than 5%. Treatment has not changed much in the last decade or so, with surgical resection and radio/chemotherapy being the main options. Glioblastoma is highly heterogeneous and frequently becomes treatment-resistant due to the ability of glioblastoma cells to adopt stem cell states facilitating tumor recurrence. Therefore, there is an urgent need for novel therapeutic strategies. The ubiquitin system, in particular E3 ubiquitin ligases and deubiquitinating enzymes, have emerged as a promising source of novel drug targets. In addition to conventional small molecule drug discovery approaches aimed at modulating enzyme activity, several new and exciting strategies are also being explored. Among these, PROteolysis TArgeting Chimeras (PROTACs) aim to harness the endogenous protein turnover machinery to direct therapeutically relevant targets, including previously considered “undruggable” ones, for proteasomal degradation. PROTAC and other strategies targeting the ubiquitin proteasome system offer new therapeutic avenues which will expand the drug development toolboxes for glioblastoma. This review will provide a comprehensive overview of E3 ubiquitin ligases and deubiquitinating enzymes in the context of glioblastoma and their involvement in core signaling pathways including EGFR, TGF-β, p53 and stemness-related pathways. Finally, we offer new insights into how these ubiquitin-dependent mechanisms could be exploited therapeutically for glioblastoma.
Reactive Oxygen Species Interact With NLRP3 Inflammasomes and Are Involved in the Inflammation of Sepsis: From Mechanism to Treatment of Progression
Authors: Zhao Shuai , Chen Fan , Yin Qiliang , Wang Dunwei , Han Wei , Zhang Yuan
Published: November 2020

Over the past 10 years, the crisis of sepsis has remained a great challenge. According to data from 2016, the sepsis-related mortality rate remains high. In addition, sepsis consumes extensive medical resources in intensive care units, and anti-inflammatory agents fail to improve sepsis-associated hyperinflammation and symptoms of immunosuppression. The specific immune mechanism of sepsis remains to be elucidated. Reactive oxygen species (ROS) are triggered by energy metabolism and respiratory dysfunction in sepsis, which not only cause oxidative damage to tissues and organelles, but also directly and indirectly promote NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) inflammasome activation. NLRP3 inflammasomes enlarge the inflammatory response and trigger apoptosis of immune cells to exacerbate sepsis progression. Inhibiting the negative effects of ROS and NLRP3 inflammasomes therefore provides the possibility of reversing the excessive inflammation during sepsis. In this review, we describe the interaction of ROS and NLRP3 inflammasomes during sepsis, provide prevention strategies, and identify fields that need further study.
The Role of Basement Membranes in Cerebral Amyloid Angiopathy
Authors: Howe Matthew D. , McCullough Louise D. , Urayama Akihiko
Published: November 2020

Dementia is a neuropsychiatric syndrome characterized by cognitive decline in multiple domains, often leading to functional impairment in activities of daily living, disability, and death. The most common causes of age-related progressive dementia include Alzheimer’s disease (AD) and vascular cognitive impairment (VCI), however, mixed disease pathologies commonly occur, as epitomized by a type of small vessel pathology called cerebral amyloid angiopathy (CAA). In CAA patients, the small vessels of the brain become hardened and vulnerable to rupture, leading to impaired neurovascular coupling, multiple microhemorrhage, microinfarction, neurological emergencies, and cognitive decline across multiple functional domains. While the pathogenesis of CAA is not well understood, it has long been thought to be initiated in thickened basement membrane (BM) segments, which contain abnormal protein deposits and amyloid-β (Aβ). Recent advances in our understanding of CAA pathogenesis link BM remodeling to functional impairment of perivascular transport pathways that are key to removing Aβ from the brain. Dysregulation of this process may drive CAA pathogenesis and provides an important link between vascular risk factors and disease phenotype. The present review summarizes how the structure and composition of the BM allows for perivascular transport pathways to operate in the healthy brain, and then outlines multiple mechanisms by which specific dementia risk factors may promote dysfunction of perivascular transport pathways and increase Aβ deposition during CAA pathogenesis. A better understanding of how BM remodeling alters perivascular transport could lead to novel diagnostic and therapeutic strategies for CAA patients.
Complexity-Based Decoding of the Coupling Among Heart Rate Variability (HRV) and Walking Path
Authors: Mujib Kamal Shahul , Babini Mohammad Hossein , Krejcar Ondrej , Namazi Hamidreza
Published: November 2020

Walking is an everyday activity in our daily life. Because walking affects heart rate variability, in this research, for the first time, we analyzed the coupling among the alterations of the complexity of walking paths and heart rate. We benefited from the fractal theory and sample entropy to evaluate the influence of the complexity of paths on the complexity of heart rate variability (HRV) during walking. We calculated the fractal exponent and sample entropy of the R-R time series for nine participants who walked on four paths with various complexities. The findings showed a strong coupling among the alterations of fractal dimension (an indicator of complexity) of HRV and the walking paths. Besides, the result of the analysis of sample entropy also verified the obtained results from the fractal analysis. In further studies, we can analyze the coupling among the alterations of the complexities of other physiological signals and walking paths.
Rapid Manipulation in Irradiance Induces Oxidative Free-Radical Release in a Fast-Ice Algal Community (McMurdo Sound, Antarctica)
Authors: Kennedy Fraser , Martin Andrew , Castrisios Katerina , Cimoli Emiliano , McMinn Andrew , Ryan Ken G.
Published: November 2020

Sea ice supports a unique assemblage of microorganisms that underpin Antarctic coastal food-webs, but reduced ice thickness coupled with increased snow cover will modify energy flow and could lead to photodamage in ice-associated microalgae. In this study, microsensors were used to examine the influence of rapid shifts in irradiance on extracellular oxidative free radicals produced by sea-ice algae. Bottom-ice algal communities were exposed to one of three levels of incident light for 10 days: low (0.5 μmol photons m−2 s−1, 30 cm snow cover), mid-range (5 μmol photons m−2 s−1, 10 cm snow), or high light (13 μmol photons m−2 s−1, no snow). After 10 days, the snow cover was reversed (either removed or added), resulting in a rapid change in irradiance at the ice-water interface. In treatments acclimated to low light, the subsequent exposure to high irradiance resulted in a ~400× increase in the production of hydrogen peroxide (H2O2) and a 10× increase in nitric oxide (NO) concentration after 24 h. The observed increase in oxidative free radicals also resulted in significant changes in photosynthetic electron flow, RNA-oxidative damage, and community structural dynamics. In contrast, there was no significant response in sea-ice algae acclimated to high light and then exposed to a significantly lower irradiance at either 24 or 72 h. Our results demonstrate that microsensors can be used to track real-time in-situ stress in sea-ice microbial communities. Extrapolating to ecologically relevant spatiotemporal scales remains a significant challenge, but this approach offers a fundamentally enhanced level of resolution for quantifying the microbial response to global change.
Young Patients With Persistent and Complex Care Needs Require an Integrated Care Approach: Baseline Findings From the Multicenter Youth Flexible ACT Study
Authors: Broersen Marieke , Frieswijk Nynke , Kroon Hans , Vermulst Ad A. , Creemers Daan H. M.
Published: November 2020

Background: The Multicenter Youth Flexible ACT Study is an ongoing observational prospective cohort study that examines the effects of Youth Flexible ACT (Assertive Community Treatment) on young people with complex care needs who are difficult to engage in traditional (office-based) mental health services. However, a clear and detailed description of this patient group is lacking. In the current paper, we present baseline characteristics and psychosocial outcomes of the Youth Flexible ACT target group and explore the existence of underlying specific patient subgroups.

Methods: Sixteen Youth Flexible ACT teams from seven mental healthcare institutes in the Netherlands participated in the study. Research participants were monitored for 18 months and administered questionnaires measuring psychiatric- and social functioning every 6 months, yielding four measurements. Baseline data were obtained from 199 adolescents, their mental health workers, and parents/carers. Latent Class Analysis based on HoNOSCA scores (measuring psychosocial and daily functioning) was conducted to identify underlying subgroups.

Results: The target group of Youth Flexible ACT mainly consisted of patients older than 15 years of age with a history of (specialized) mental healthcare. They face many complex problems, including trauma; developmental, mood, and anxiety disorders; and problems with school attendance, family life, and peer relationships. Other frequently reported difficulties were substance misuse, the involvement of the legal system or police, problems with intellectual functioning, and personal finance. Patients were classified into four distinct classes: the “internalizing,” “externalizing,” “non-specific,” and the “overly impulsive” subgroup. Each subgroup had its unique pattern of difficulties and focus, respectively, high levels of depression and anxiety, disruptive behavior, unspecific difficulties, and substance misuse.

Conclusions: As expected, patients in Youth Flexible ACT experienced many severe problems, rendering them vulnerable to fragmented and, thus, ineffective care. Our findings underscore the need for an integrated care approach with a multidisciplinary team of skilled professionals that can bridge these wide-ranging psychosocial problems, as each class of participants experienced a different set of difficulties. Youth Flexible ACT teams need to adjust their care services accordingly.
Estimating Walking Speed in the Wild
Authors: Baroudi Loubna , Newman Mark W. , Jackson Elizabeth A. , Barton Kira , Shorter K. Alex , Cain Stephen M.
Published: November 2020

An individual's physical activity substantially impacts the potential for prevention and recovery from diverse health issues, including cardiovascular diseases. Precise quantification of a patient's level of day-to-day physical activity, which can be characterized by the type, intensity, and duration of movement, is crucial for clinicians. Walking is a primary and fundamental physical activity for most individuals. Walking speed has been shown to correlate with various heart pathologies and overall function. As such, it is often used as a metric to assess health performance. A range of clinical walking tests exist to evaluate gait and inform clinical decision-making. However, these assessments are often short, provide qualitative movement assessments, and are performed in a clinical setting that is not representative of the real-world. Technological advancements in wearable sensing and associated algorithms enable new opportunities to complement in-clinic evaluations of movement during free-living. However, the use of wearable devices to inform clinical decisions presents several challenges, including lack of subject compliance and limited sensor battery life. To bridge the gap between free-living and clinical environments, we propose an approach in which we utilize different wearable sensors at different temporal scales and resolutions. Here, we present a method to accurately estimate gait speed in the free-living environment from a low-power, lightweight accelerometer-based bio-logging tag secured on the thigh. We use high-resolution measurements of gait kinematics to build subject-specific data-driven models to accurately map stride frequencies extracted from the bio-logging system to stride speeds. The model-based estimates of stride speed were evaluated using a long outdoor walk and compared to stride parameters calculated from a foot-worn inertial measurement unit using the zero-velocity update algorithm. The proposed method presents an average concordance correlation coefficient of 0.80 for all subjects, and 97% of the error is within ±0.2m·s−1. The approach presented here provides promising results that can enable clinicians to complement their existing assessments of activity level and fitness with measurements of movement duration and intensity (walking speed) extracted at a week time scale and in the patients' free-living environment.
Specific Detection and Differentiation Between Brucella melitensis and Brucella abortus by a Duplex Recombinase Polymerase Amplification Assay
Authors: Gumaa M. M. , Li Zhaocai , Cao Xiaoan , Zhang Nianzhang , Lou Zhongzi , Zhou Jizhang , Fu Baoquan
Published: November 2020

Brucellosis is a highly contagious zoonosis caused by a species under the genus Brucella. A duplex recombinase polymerase amplification (Duplex RPA) assay for the specific detection of Brucella melitensis and Brucella abortus was developed in this study. Primers were designed targeting hypothetical protein genes and membrane transporter genes of B. melitensis and B. abortus, respectively. The newly developed assay was validated for its analytical sensitivity and specificity. Different samples were collected from the Qinghai, Inner Mongolia, and Xinjiang provinces. After DNA extraction, the samples were analyzed by Duplex RPA, real-time PCR, and multiplex AMOS PCR to estimate the prevalence of brucellosis in sheep and yak in West China. The analytical sensitivities of Duplex RPA were 9 × 102 plasmid copies of B. melitensis and 9 × 101 plasmid copies of B. abortus, but by mixing the reaction tubes after 4 min of incubation, the sensitivities were 4 × 100 and 5 × 100 copies of B. melitensis and B. abortus, respectively. There was no cross-reactivity with Brucella suis, Chlamydia abortus, Salmonella typhimurium, Escherichia coli, and Toxoplasma gondii. The screening of field samples by Duplex RPA revealed that the prevalence of B. melitensis in sheep and yak was 75.8% and the prevalence of B. abortus was 4.8%. Multiplex AMOS PCR showed that the prevalence of B. melitensis was 19.3%, and that of B. abortus was 4.8%. It was concluded that the developed Duplex RPA is sensitive and specific to the detection of and differentiation between B. melitensis and B. abortus which will be useful in epidemiological surveillance and in the clinical settings.
Enhancing Healthcare Decision-Making Process: Findings from Orthopaedic Field
Authors: Schettini Irene , Palozzi Gabriele , Chirico Antonio
Published: November 2020

In the healthcare field, the decision-making process is part of the broad spectrum of “clinical reasoning”, which is recognised as the whole process by which a physician decides about patients’ treatments and cares. Several clinicians’ intrinsic variables lead to this decisional path. Little is known about the inference of these variables in triggering biases in decisions about the post-discharge period in the surgical field. Accordingly, this research aims to understand if and how cognitive biases can affect orthopaedists in decision-making regarding the follow-up after knee and hip arthroplasty. To achieve this goal, an interview-based explorative case study was run. Three key-decisional orthopaedic surgeons were interviewed through a quality control tool aimed at monitoring the causes and effects of cognitive distortions. Coherently with the literature, eight biases come to light. All the interviewees agree on the presence of four common biases in orthopaedic surgery (Affect heuristic, Anchoring, Halo effect, Saliency). The other biases (Groupthink, Availability, Overconfidence, Confirmation), instead, depending on specific physicians’ intrinsic variables; namely: (i) working experience; (ii) working context. This finding contributes to the debate about the application of cognitive tools as leverage for improving the quality of clinical decision-making process and, indirectly, enhancing better healthcare outcomes.
Maize Yield Components as Affected by Plant Population, Planting Date and Soil Coverings in Brazil
Authors: Beruski Gustavo Castilho , Schiebelbein Luis Miguel , Pereira André Belmont
Published: November 2020

The potential yield of annual crops is affected by management practices and water and energy availabilities throughout the crop season. The current work aimed to assess the effects of plant population, planting dates and soil covering on yield components of maize. Field experiments were carried out during the 2014–2015 and 2015–2016 growing seasons at areas grown with oat straw, voluntary plants and bare soil, considering five plant populations (40,000, 60,000, 80,000, 100,000 and 120,000 plants ha−1) and three sowing dates (15 September, 30 October and 15 December) for the hybrid P30F53YH in Ponta Grossa, State of Paraná, Brazil. Non-impacts of soil covering or plant population on plant height at the flowering phenological stage were observed. Significant effects of soil covering on yield components and final yield responses throughout the 2014–2015 season were detected. An influence of plant populations on yield components was evidenced, suggesting that, from 80,000 plants ha−1, the P30F53YH hybrid performs a compensatory effect among assessed yield components in such a way as to not compromise productivity insofar as the plant population increases up to 120,000 plants ha−1. It was noticed, a positive trend of yield components and crop final yield as a function of plant density increments.
The Cultivation Techniques and Quality Characteristics of a New Germplasm of Vitis adenoclada Hand.-Mazz Grape
Authors: Wu Dai-Dong , Cheng Guo , Li Hong-Yan , Zhou Si-Hong , Yao Ning , Zhang Jin , Xie Lin-Jun
Published: November 2020

Vitis adenoclada Hand.-Mazz is a special wild grape resource that is often confused with Vitis heyneana Roem. & Schult in research or production practice, and there are few comprehensive studies on this species in recent years. “Gui Heizhenzhu No. 3” (GH3), as a new germplasm of V. adenoclada found in Guangxi, China, has many advantages, such as good quality and strong adaptability. In this paper, an attempt was made to introduce the breeding process of GH3, including a brief description of its botanical characteristics and its cultivation and management techniques in karst rocky desertification mountains. Meanwhile, its quality-related parameters were evaluated by widely targeted metabolomic analysis. This study indicated that GH3 had the typical botanical characteristics of V. adenoclada, but with larger fruit and a higher sugar content compared to wild or other V. adenoclada grape varieties. Metabolomic study of the target variety showed that glucose and citric acid were the main sugar and acid components in fully ripened berries. Moreover, cyanidin-3-O-glucoside presented as the characteristic anthocyanin. In addition, B-ring dihydroxylation was more active than trihydroxylation in the GH3 berry. Several of its botanical and quality characteristics highlight the unique genetic background of this variety. Thus, it has an important guiding significance and a scientific theoretical basis for identifying, exploiting, and utilizing East Asian wild grape resources.
Differential Ozone Responses Identified among Key Rust-Susceptible Wheat Genotypes
Authors: Mashaheet Alsayed M. , Burkey Kent O. , Saitanis Costas J. , Abdelrhim Abdelrazek S. , Rafiullah  , Marshall David S.
Published: November 2020

Increasing ambient ozone (O3) concentrations and resurgent rust diseases are two concomitant limiting factors to wheat production worldwide. Breeding resilient wheat cultivars bearing rust resistance and O3 tolerance while maintaining high yield is critical for global food security. This study aims at identifying ozone tolerance among key rust-susceptible wheat genotypes [Rust near-universal susceptible genotypes (RnUS)], as a first step towards achieving this goal. Tested RnUS included seven bread wheat genotypes (Chinese Spring, Line E, Little Club, LMPG 6, McNair 701, Morocco and Thatcher), and one durum wheat line (Rusty). Plants were treated with five O3 concentrations (CF, 50, 70, 90, and 110 ppb), in two O3 exposure systems [continuous stirred tank reactors (CSTR) and outdoor-plant environment chambers (OPEC)], at 21–23 Zadoks decimal growth stage. Visible injury and biomass accumulation rate were used to assess O3 responses. Visible injury data showed consistent order of genotype sensitivity (Thatcher, LMPG 6 > McNair 701, Rusty > Line E, Morocco, Little Club > Chinese Spring). Additionally, leaves at different orders showed differential O3 responses. Biomass accumulation under O3 stress showed similar results for the bread wheat genotypes. However, the durum wheat line “Rusty” had the most O3-sensitive biomass production, providing a contrasting O3 response to the tolerance reported in durum wheat. Chinese Spring was the most tolerant genotype based on both parameters and could be used as a source for O3 tolerance, while sensitive genotypes could be used as sensitive parents in mapping O3 tolerance in bread wheat. The suitability of visible symptoms and biomass responses in high-throughput screening of wheat for O3 tolerance was discussed. The results presented in this research could assist in developing future approaches to accelerate breeding wheat for O3 tolerance using existing breeding materials.
Delineation of Management Zones for Site-Specific Information about Soil Fertility Characteristics through Proximal Sensing of Potato Fields
Authors: Khan Humna , Farooque Aitazaz A. , Acharya Bishnu , Abbas Farhat , Esau Travis J. , Zaman Qamar U.
Published: November 2020

The delineation of management zones (MZs) has been suggested as a solution to mitigate adverse impacts of soil variability on potato tuber yield. This study quantified the spatial patterns of variability in soil and crop properties to delineate MZs for site-specific soil fertility characterization of potato fields through proximal sensing of fields. Grid sampling strategy was adopted to collect soil and crop data from two potato fields in Prince Edward Island (PEI). DUALEM-2 sensor, Time Domain Reflectometry (TDR-300), GreenSeeker were used to collect soil ground conductivity parameter horizontal coplanar geometry (HCP), soil moisture content (θ), and normalized difference vegetative index (NDVI), respectively. Soil organic matter (SOM), soil pH, phosphorous (P), potash (K), iron (Fe), lime index (LI), and cation exchange capacity (CEC) were determined from soil samples collected from each grid. Stepwise regression shortlisted the major properties of soil and crop that explained 71 to 86% of within-field variability. The cluster analysis grouped the soil and crop data into three zones, termed as excellent, medium, and poor at a 40% similarity level. The coefficient of variation and the interpolated maps characterized least to moderate variability of soil fertility parameters, except for HCP and K that were highly variable. The results of multiple means comparison indicated that the tuber yield and HCP were significantly different in all MZs. The significant relationship between HCP and yield suggested that the ground conductivity data could be used to develop MZs for site-specific fertilization in potato fields similar to those used in this study.
Train Scheduling with Deep Q-Network: A Feasibility Test
Authors: Gong Intaek , Oh Sukmun , Min Yunhong
Published: November 2020

We consider a train scheduling problem in which both local and express trains are to be scheduled. In this type of train scheduling problem, the key decision is determining the overtaking stations at which express trains overtake their preceding local trains. This problem has been successfully modeled via mixed integer programming (MIP) models. One of the obvious limitation of MIP-based approaches is the lack of freedom to the choices objective and constraint functions. In this paper, as an alternative, we propose an approach based on reinforcement learning. We first decompose the problem into subproblems in which a single express train and its preceding local trains are considered. We, then, formulate the subproblem as a Markov decision process (MDP). Instead of solving each instance of MDP, we train a deep neural network, called deep Q-network (DQN), which approximates Q-value function of any instances of MDP. The learned DQN can be used to make decision by choosing the action which corresponds to the maximum Q-value. The advantage of the proposed method is the ability to incorporate any complex objective and/or constraint functions. We demonstrate the performance of the proposed method by numerical experiments.
Spectral Alteration Zonation Based on Close Range HySpex-320 m Imaging Spectroscopy: A Case Study in the Gongchangling High-Grade Iron Ore Deposit, Liaoning Province, NE China
Authors: Song Kun , Wang Ende , Yao Yuzeng , Fu Jianfei , Hao Dahai , You Xinwei
Published: November 2020

Research on wall rock alteration is of great importance to the understanding and exploration of ore deposits. The microscopic changes of the same mineral in different alteration zones can provide information about the migration and enrichment of ore-forming elements. In this paper, a typical profile of a high-grade iron ore body in Gongchangling iron deposit was investigated and sampled. The samples were checked by polarized microscopy, and alterations zonation were delineated according to the hydrothermal mineral assemblages and paragenesis. Moreover, hyperspectral images of wall rocks from each alteration zone were obtained by Norsk Elektro Optikk (NEO) HySpex-320 m imaging system. A customer Interactive Data Language (IDL) software package was used to process the images, and spectral features were extracted from the selected samples. The results indicate that spectral characteristics manifest obviously regular variations; i.e., from proximal to distal for the high-grade iron ore body, the wavelengths at ca. 1200 nm of chlorite and garnet, which accounts for most of the hydrothermal alteration minerals, become longer, and the absorption depths gradually smaller. The spectral features at 1200 nm of chlorite and garnet are always caused by the crystal field effect of Fe2+; therefore, the wavelength variations indicate the increase of Fe2+ and a reduced environment, which can provide more detailed information about the metallogeny and water–rock interaction. Since the hyperspectral features of the altered rocks can disclose unique mineralogical and structural information, the conventional classification of alteration zonation should be combined with the spectral feature, i.e., spectral alteration zonation, which is of great help to the understanding of the forming conditions of wall rock alteration and also the high-grade iron ore bodies.
Antibacterial Bio-Based Polymers for Cranio-Maxillofacial Regeneration Applications
Authors: Martín-del-Campo Marcela , Fernández-Villa Daniel , Cabrera-Rueda Gabriela , Rojo Luis
Published: November 2020

Cranio-maxillofacial structure is a region of particular interest in the field of regenerative medicine due to both its anatomical complexity and the numerous abnormalities affecting this area. However, this anatomical complexity is what makes possible the coexistence of different microbial ecosystems in the oral cavity and the maxillofacial region, contributing to the increased risk of bacterial infections. In this regard, different materials have been used for their application in this field. These materials can be obtained from natural and renewable feedstocks, or by synthetic routes with desired mechanical properties, biocompatibility and antimicrobial activity. Hence, in this review, we have focused on bio-based polymers which, by their own nature, by chemical modifications of their structure, or by their combination with other elements, provide a useful antibacterial activity as well as the suitable conditions for cranio-maxillofacial tissue regeneration. This approach has not been reviewed previously, and we have specifically arranged the content of this article according to the resulting material and its corresponding application; we review guided bone regeneration membranes, bone cements and devices and scaffolds for both soft and hard maxillofacial tissue regeneration, including hybrid scaffolds, dental implants, hydrogels and composites.