bluuebunny/crossref_metadata_2025 huggingface dataset:
LINGUISTIC ASPECT OF INTERCULTURAL CONFLICT COMMUNICATION
Authors: Harbera Iryna Volodymyrivna
Published: February 2024

The article reveals the linguistic aspect of intercultural conflict communication with the help of a specially prepared linguistic experiment on “Interlingual homonyms” (conducted with English- and Ukrainian-speaking students of Vasyl’ Stus Donetsk National University), as well as through scientific observation (interesting conflict-generating communication situations during the educational process, the subject of which was the contradictory use of certain morphological forms and phraseological units, were identified). Intercultural communication is positioned as a process of verbal and non-verbal communication between people belonging to different national and cultural communities. Conflict in intercultural communication (i.e., intercultural conflict communication) is presented as a cultural phenomenon, the content of which is determined by cultural factors, and the process of emergence and development of conflicts is regulated by cultural norms, which under certain circumstances lead to the emergence of special cultural rituals that affect the process of conflict interaction between participants. The analysis of the linguistic aspect of intercultural conflict communication is to identify verbal and non-verbal markers of cultural information in order to achieve communicative interaction between representatives of different cultures and prevent the emergence of communicative conflicts. Specific examples demonstrate that intercultural conflict communication can be realized at several linguistic levels: lexical, phraseological, morphological, and syntactic. It is proved that the most effective way to regulate intercultural conflict communication is to develop intercultural communication competence in its real and potential participants as the ability to adequately use culturally significant language tools, concepts and behavioral stereotypes.
Evaluation of transfection with gemini nanoparticles for retinal neuroprotection in a dynamic microfluidic cell culture system
Authors: Narsineni Lokesh , Hakim Antoine , Guido Benjamin , Foldvari Marianna
Published: February 2024

Glaucoma is a neurodegenerative disease of the retina and optic nerve with elevated intraocular pressure as one of the main contributing factors. The traditional in vitro methods to evaluate new gene delivery systems for neuroprotective treatments of glaucoma require testing in cell monolayers under static conditions. Therefore, a microfluidic technology that provides a dynamic simulation of cell exposure to nanoparticles (NPs) could be a useful model for rapid screening. In this study, CellASIC ONIX2 microfluidic platform was used to evaluate and optimize culture and transfection conditions to assess cell viability and transfection efficiency (TE) of gemini NPs carrying GFP-encoding plasmid in A7 astrocytes, one of the targeted retinal cells for glaucoma treatment. The average TE (inlet-middle-outlet of culture chambers) by gemini nanoplexes, 18-7NH-18 NPXs and 18-7Np3-18 NPXs was > 85% and > 25%, respectively, after 5 h of perfusion with a combined average FI (caFI) of about 600 and < 100, respectively, whereas TE after Lipofectamine 3000 was > 70% and caFI of < 100. The results have shown that comparatively to 2D monolayers, the microfluidics technique provides better exposure of cells to NPs, suitable for kinetic mapping of gene expression, and a useful tool for cell growth and live evaluation of gene delivery system interaction with cells.
Stochastic models of work and rest schedules
Authors: Bochkovskyi A. P
Published: February 2024

Purpose. To develop stochastic models for determining the duration of work and rest schedules that ensure protection of workers from occupational hazards and a high level of labor productivity during a work shift. Methodology. Analysis of scientific literature was applied to determine the purpose and tasks of the research; methods of formalization – to describe the characteristics, dynamics and states of random processes of accumulation and elimination of the consequences of the negative impact of harmful production factors occurring in an worker within the work and rest schedules; methods of semi-Markov processes theory, theories of reliability and recovery – to build stochastic models of work and rest schedules, determine the conditions and probability of a stable mode of their operation. Findings. Stochastic models were developed to determine the duration of work schedules, at the end of which the level of accumulation of the consequences of the negative impact of harmful production factors in the worker will not exceed the set maximum permissible values with a high probability. And such durations of rest schedules, at the end of which this level will be equal to zero (also with a high probability). The condition for ensuring the stable operation of these schedules throughout the entire period of work experience at the workplace was determined by the author. Originality. For the first time an approach to the development of work and rest schedules at workplaces is proposed, which, contrary to others, is based on determining the probabilities of the levels of accumulation of the consequences of the negative impact of harmful production factors in the worker at the end of these schedules, given the actual random and dynamic characteristics of such impact. This, in turn, allows ensuring the protection of the worker both from accidents caused by the fatigue and from professional illness. Practical value. The proposed approach allows increasing the economic efficiency of the enterprise by ensuring a high level of labor productivity, which is achieved by simultaneous development of the maximum possible duration of work and the minimum possible duration of rest schedules, which exclude the worker’s development and occurrence of fatigue and professional illness with a high probability.
Evaluation of the antimalarial activity of SAM13-2HCl with morpholine amide (SKM13 derivative) against antimalarial drug-resistant Plasmodium falciparum and Plasmodium berghei infected ICR mice
Authors: Hong Hyelee , Moon Kwonmo , Trinh Thuy-Tien Thi , Eom Tae-Hui , Park Hyun , Kim Hak Sung , Yeo Seon-Ju
Published: February 2024

Antimalarial drugs are an urgently need and crucial tool in the campaign against malaria, which can threaten public health. In this study, we examined the cytotoxicity of the 9 antimalarial compounds chemically synthesized using SKM13-2HCl. Except for SKM13-2HCl, the 5 newly synthesized compounds had a 50% cytotoxic concentration (CC50) > 100 µM, indicating that they would be less cytotoxic than SKM13-2HCl. Among the 5 compounds, only SAM13-2HCl outperformed SKM13-2HCl for antimalarial activity, showing a 3- and 1.3-fold greater selective index (SI) (CC50/IC50) than SKM13-2HCl in vitro against both chloroquine-sensitive (3D7) and chloroquine -resistant (K1) Plasmodium falciparum strains, respectively. Thus, the presence of morpholine amide may help to effectively suppress human-infectious P. falciparum parasites. However, the antimalarial activity of SAM13-2HCl was inferior to that of the SKM13-2HCl template compound in the P. berghei NK65-infected mouse model, possibly because SAM13-2HCl had a lower polarity and less efficient pharmacokinetics than SKM13-2HCl. SAM13-2HCl was more toxic in the rodent model. Consequently, SAM13-2HCl containing morpholine was selected from screening a combination of pharmacologically significant structures as being the most effective in vitro against human-infectious P. falciparum but was less efficient in vivo in a P. berghei-infected animal model when compared with SKM13-2HCl. Therefore, SAM13-2HCl containing morpholine could be considered a promising compound to treat chloroquine-resistant P. falciparum infections, although further optimization is crucial to maintain antimalarial activity while reducing toxicity in animals.
Infection characteristics of Metagonimus species (Digenea: Heterophyidae) metacercariae in fish from major rivers of Korea
Authors: Sohn Woon-Mok
Published: February 2024

This article analyzed the infection characteristics of metacercariae of Metagonimus spp. (MsMc) in fish from 9 major water systems in Korea. A total of 19,568 fish in 87 species were examined over a period of 10 years (2011–2020). MsMc were detected in fish from all 44 survey areas in 9 water systems. Most of the surveyed sites showed very low and low infection levels (66.7%), while 33.3% of the areas, such as Tamjin-gang and Seomjin-gang, revealed moderate and high infection levels. High endemicity depends on the abundance of susceptible fish species, especially sweet smelt (Plecoglosus altivelis). The susceptibility index (SI) with MsMc in index fish, Zacco spp., was very low and low levels in 62.0%, moderate in 28.0%, and high in 10.0% regions. The SI was highest in the following order: Yeongam-cheon (283.8), Hoeng-cheon (192.3), Togyo-jeosuji (131.2), Deokcheon-gang (119.1), and Joyang-gang (106.3). The recent infection status of MsMc in P. altivelis was analyzed by the survey localities. In addition, except for P. altivelis, 9 fish species were highly infected with MsMc in some survey areas, including Zacco platypus, Z. koreanus, Z. temminckii, Opsariichthys uncirostris, Rhynchocypris oxycephalus, Carassius auratus, Acheilognathus rhombeus, Onchorhynchus masou, and Tribolodon hakonensis. In Korea, 74 fish species (15 families) are collectively listed as second intermediate hosts of Metagonimus spp. This review provides several novel characteristics of MsMc infection and clarifies the fish species of second intermediate host of Metagonimus spp. in this country.
In vitro immunoregulatory role of recombinant Ancylostoma ceylanicum calreticulin
Authors: Zhuang Tingting , Abuzeid Asmaa M. I. , Chen Xiaoyu , Zhu Shilan , Li Guoqing
Published: February 2024

Ancylostoma ceylanicum is a zoonotic soil-derived nematode that parasitizes the intestines of humans and animals (dogs and cats), leading to malnutrition and iron-deficiency anemia. Helminth parasites secrete calreticulin (CRT), which regulates or blocks the host’s immune response. However, no data on A. ceylanicum calreticulin (Ace-CRT) are available. We investigated the biological function of recombinant Ace-CRT (rAce-CRT). rAce-CRT showed reliable antigenicity and stimulated the proliferation of mouse splenocytes and canine peripheral blood mononuclear cells. Quantitative reverse-transcription PCR assays revealed that rAce-CRT primarily promoted the expression of T helper 2 cytokines, particularly IL-13, in canine peripheral blood lymphocytes. rAce-CRT inhibited complement-mediated sheep erythrocyte hemolysis in vitro. Our findings indicate that Ace-CRT plays an immunomodulatory role and may be a promising candidate molecule for a hookworm vaccine.
Molecular detection and characterization of Acanthamoeba infection in dogs and its association with keratitis in Korea
Authors: Lee Subin , Alkathiri Badriah , Jung Ji Seung , Kang Nanyoung , Hwang Jiyi , Park Sang-Eun , Hong Yeonchul , Park Kyung-Mee , Lee Seung-Hun
Published: February 2024

Acanthamoeba infection is associated with keratitis in humans; however, its association with keratitis in dogs remains unclear. To investigate this possibility, we collected 171 conjunctival swab samples from dogs with eye-related diseases (65 with keratitis and 106 without keratitis) at Chungbuk National University Veterinary Teaching Hospital, Korea, from August 2021 to September 2022. Polymerase chain reaction identified 9 samples (5.3%) as Acanthamoeba positive; of these, 3 were from dogs with keratitis (4.6%) and 6 were from dogs without keratitis (5.7%). Our results indicated no significant association between Acanthamoeba infection and keratitis, season, sex, or age. All Acanthamoeba organisms found in this study had the genotype T4, according to 18S ribosomal RNA analysis. Acanthamoeba infection in dogs might have only a limited association with keratitis.
Genome-wide identification of histone lysine methyltransferases and their implications in the epigenetic regulation of eggshell formation-related genes in a trematode parasite Clonorchis sinensis
Authors: Park Min-Ji , Sohn Woon-Mok , Bae Young-An
Published: February 2024

Epigenetic writers including DNA and histone lysine methyltransferases (DNMT and HKMT, respectively) play an initiative role in the differentiation and development of eukaryotic organisms through the spatiotemporal regulation of functional gene expressions. However, the epigenetic mechanisms have long been suspected in helminth parasites lacking the major DNA methyltransferases DNMT1 and DNMT3a/3b. Very little information on the evolutionary status of the epigenetic tools and their role in regulating chromosomal genes is currently available in the parasitic trematodes. We previously suggested the probable role of a DNMT2-like protein (CsDNMT2) as a genuine epigenetic writer in a trematode parasite Clonorchis sinensis. Here, we analyzed the phylogeny of HKMT subfamily members in the liver fluke and other platyhelminth species. The platyhelminth genomes examined conserved genes for the most of SET domain-containing HKMT and Disruptor of Telomeric Silencing 1 subfamilies, while some genes were expanded specifically in certain platyhelminth genomes. Related to the high gene dosages for HKMT activities covering differential but somewhat overlapping substrate specificities, variously methylated histones were recognized throughout the tissues/organs of C. sinensis adults. The temporal expressions of genes involved in eggshell formation were gradually decreased to their lowest levels proportionally to aging, whereas those of some epigenetic tool genes were re-boosted in the later adult stages of the parasite. Furthermore, these expression levels were significantly affected by treatment with DNMT and HKMT inhibitors. Our data strongly suggest that methylated histones are potent epigenetic markers that modulate the spatiotemporal expressions of C. sinensis genes, especially those involved in sexual reproduction.
Current Approaches for the Diagnosis and Conservative Treatment of Stress Urinary Incontinence - A Guideline of Guidelines
Authors: Kaynar Bedriye Muge , Kalkan Senad
Published: February 2024

Urologists utilize evidence-based guidelines organized by urological organizations in the management of stress urinary incontinence (SUI). The objective of this study is to provide guidance in the clinical management of stress urinary incontinence (SUI) by reviewing key guidelines. We conducted a medical literature analysis in the following databases: PubMed, Medline, Embase, National Guideline Clearinghouse, the National Institute for Health and Care Excellence, and Cochrane Library. We also manually searched the websites of the following international and national societies to identify relevant guidelines for inclusion in this review: the International Consultation on Incontinence, American College of Obstetrics and Gynecology, American Urogynecologic Society, American Urological Association/Society of Urodynamic, Female Pelvic Medicine and Urogenital Reconstruction, National Institute for Health and Care Excellence, European Association of Urology, and Canadian Urological Association. The recommendations in the guidelines are summarized in different areas, including the diagnostic standards of SUI, examination and evaluation methods, and conservative treatment methods. This ‘guideline of guidelines’ presents the similarities and differences between prominent authorities in the management of SUI.
A new bird calicivirus detected in feces of cormorants
Authors: Wu Yan , Lu Chunying , Zhao Ran , He Yuyun , Hou Jingjing , Sun Yijie , Yang Shixing , Qin Zhaoying , Ji Likai , Wang Yan , Zhang Wen
Published: February 2024

The incidence of reporting caliciviruses in wild birds is less common than in other animals, and the majority of cases remain unclassified. A strain of calicivirus was discovered in this study in the feces of cormorants collected at Xiamen Horticulture Expo Garden in 2021 and was named Cormcali01. The genome of Cormcali01 was 8,561 bp in length which contained characteristic motifs present in other caliciviruses. Furthermore, it demonstrated a significant deviation from all existing calicivirus nucleotide sequences, exhibiting the highest amino acid identity (47.34%) to the unclassified Ruddy turnstone calicivirus A. A pairwise comparison of the VP1 protein showed that Cormcali01 had the highest amino acid identity of 43.90% with the unassigned Ruddy turnstone calicivirus A. Phylogenetic analysis demonstrated that VP1 of Cormcali01 clustered with unassigned caliciviruses. Therefore, based on phylogenetic analysis and pairwise comparison, Cormcali01 should be affiliated with the unassigned calicivirus, which were suggested to comprise a new calicivirus genus, the Sanovirus genus. After investigating the prevalence of Cormcali01, we discovered that 22.22% of fecal samples (10/45) were tested positive. These findings expand our understanding of the genetic variation of caliciviruses and provide valuable epidemiological information regarding a potential outbreak of calicivirus disease in birds.
The role of macrophage polarization in tendon healing and therapeutic strategies: Insights from animal models
Authors: Wang Yicheng , Lu Xiao , Lu Jianxi , Hernigou Philippe , Jin Fangchun
Published: February 2024

Tendon injuries, a common musculoskeletal issue, usually result in adhesions to the surrounding tissue, that will impact functional recovery. Macrophages, particularly through their M1 and M2 polarizations, play a pivotal role in the inflammatory and healing phases of tendon repair. In this review, we explore the role of macrophage polarization in tendon healing, focusing on insights from animal models. The review delves into the complex interplay of macrophages in tendon pathology, detailing how various macrophage phenotypes contribute to both healing and adhesion formation. It also explores the potential of modulating macrophage activity to enhance tendon repair and minimize adhesions. With advancements in understanding macrophage behavior and the development of innovative biomaterials, this review highlights promising therapeutic strategies for tendon injuries.
A developmental atlas of male terminalia across twelve species of Drosophila
Authors: Urum Anna , Rice Gavin , Glassford William , Yanku Yifat , Shklyar Boris , Rebeiz Mark , Preger-Ben Noon Ella
Published: February 2024

How complex morphologies evolve is one of the central questions in evolutionary biology. Observing the morphogenetic events that occur during development provides a unique perspective on the origins and diversification of morphological novelty. One can trace the tissue of origin, emergence, and even regression of structures to resolve murky homology relationships between species. Here, we trace the developmental events that shape some of the most diverse organs in the animal kingdom—the male terminalia (genitalia and analia) of Drosophilids. Male genitalia are known for their rapid evolution with closely related species of the Drosophila genus demonstrating vast variation in their reproductive morphology. We used confocal microscopy to monitor terminalia development during metamorphosis in twelve related species of Drosophila. From this comprehensive dataset, we propose a new staging scheme for pupal terminalia development based on shared developmental landmarks, which allows one to align developmental time points between species. We were able to trace the origin of different substructures, find new morphologies and suggest possible homology of certain substructures. Additionally, we demonstrate that posterior lobe is likely originated prior to the split between the Drosophila melanogaster and the Drosophila yakuba clade. Our dataset opens up many new directions of research and provides an entry point for future studies of the Drosophila male terminalia evolution and development.
Structure-reactivity analysis of novel hypervalent iodine reagents in S-vinylation of thiols
Authors: Doobary Sayad , Di Tommaso Ester Maria , Postole Alexandru , Inge A. Ken , Olofsson Berit
Published: February 2024

The transition-metal free S-vinylation of thiophenols by vinylbenziodoxolones (VBX) constituted an important step forward in hypervalent iodine-mediated vinylations, highlighting the difference to vinyliodonium salts and that the reaction outcome was influenced by the substitution pattern of the benziodoxolone core. In this study, we report several new classes of hypervalent iodine vinylation reagents; vinylbenziodazolones, vinylbenziodoxolonimine and vinyliodoxathiole dioxides. Their synthesis, structural and electronic properties are described and correlated to the S-vinylation outcome, shedding light on some interesting facets of these reagents.
Predicting risk of preterm birth in singleton pregnancies using machine learning algorithms
Authors: Yu Qiu-Yan , Lin Ying , Zhou Yu-Run , Yang Xin-Jun , Hemelaar Joris
Published: February 2024

We aimed to develop, train, and validate machine learning models for predicting preterm birth (<37 weeks' gestation) in singleton pregnancies at different gestational intervals. Models were developed based on complete data from 22,603 singleton pregnancies from a prospective population-based cohort study that was conducted in 51 midwifery clinics and hospitals in Wenzhou City of China between 2014 and 2016. We applied Catboost, Random Forest, Stacked Model, Deep Neural Networks (DNN), and Support Vector Machine (SVM) algorithms, as well as logistic regression, to conduct feature selection and predictive modeling. Feature selection was implemented based on permutation-based feature importance lists derived from the machine learning models including all features, using a balanced training data set. To develop prediction models, the top 10%, 25%, and 50% most important predictive features were selected. Prediction models were developed with the training data set with 5-fold cross-validation for internal validation. Model performance was assessed using area under the receiver operating curve (AUC) values. The CatBoost-based prediction model after 26 weeks' gestation performed best with an AUC value of 0.70 (0.67, 0.73), accuracy of 0.81, sensitivity of 0.47, and specificity of 0.83. Number of antenatal care visits before 24 weeks' gestation, aspartate aminotransferase level at registration, symphysis fundal height, maternal weight, abdominal circumference, and blood pressure emerged as strong predictors after 26 completed weeks. The application of machine learning on pregnancy surveillance data is a promising approach to predict preterm birth and we identified several modifiable antenatal predictors.
Relevance of mutations in protein deubiquitinases genes and TP53 in corticotroph pituitary tumors
Authors: Pękul Monika , Szczepaniak Magdalena , Kober Paulina , Rusetska Natalia , Mossakowska Beata J. , Baluszek Szymon , Kowalik Artur , Maksymowicz Maria , Zieliński Grzegorz , Kunicki Jacek , Witek Przemysław , Bujko Mateusz
Published: February 2024

Introduction

Corticotroph pituitary neuroendocrine tumors (PitNETs) develop from ACTH-producing cells. They commonly cause Cushing’s disease (CD), however, some remain clinically silent. Recurrent USP8, USP48, BRAF and TP53 mutations occur in corticotroph PitNETs. The aim of our study was to determine frequency and relevance of these mutations in a possibly large series of corticotroph PitNETs.

Methods

Study included 147 patients (100 CD and 47 silent tumors) that were screened for hot-spot mutations in USP8, USP48 and BRAF with Sanger sequencing, while 128 of these patients were screened for TP53 mutations with next generation sequencing and immunohistochemistry.

Results

USP8 mutations were found in 41% CD and 8,5% silent tumors, while USP48 mutations were found in 6% CD patients only. Both were more prevalent in women. They were related to higher rate of biochemical remission, non-invasive tumor growth, its smaller size and densely granulated histology, suggesting that these mutation may be favorable clinical features. Multivariate survival analyses did not confirm possible prognostic value of mutation in protein deubiquitinases. No BRAF mutations were found. Four TP53 mutations were identified (2 in CD, 2 in silent tumors) in tumors with size >10mm including 3 invasive ones. They were found in Crooke’s cell and sparsely granulated tumors. Tumors with missense TP53 mutations had higher TP53 immunoreactivity score than wild-type tumors. Tumor with frameshift TP53 variant had low protein expression. TP53 mutation was a poor prognostic factor in CD according to uni- and multivariate survival analyses in spite of low mutations frequency.

Conclusions

We confirmed high prevalence of USP8 mutations and low incidence of USP48 and TP53 mutations. Changes in protein deubiquitinases genes appear to be favorable prognostic factors in CD. TP53 mutations are rare, occur in both functioning and silent tumors and are related to poor clinical outcome in CD.
Optimization and analysis of distributed power carrying capacity of distribution network based on DR-DQN
Authors: Yang Zhichun , Yang Fan , Min Huaidong , Liu Yu , Zhang Nan , Zeng Hao
Published: February 2024

The booming development of distributed power sources in power systems has drawn attention to the carrying capacity and stability of the power grid, becoming a key challenge for the power industry. This study aims to develop a comprehensive deep learning model by combining deep recurrent double Q network (DR-DQN) and deep convolutional neural network (DCNN), and use meta-learning to optimize the model as a whole to simultaneously optimize the power grid. Distributed power supply carrying capacity and predicting the voltage fluctuations of the grid. The comprehensive model is designed to consider distributed power capacity optimization and voltage fluctuation prediction holistically. Through the DR-DQN model, the maximum distributed power capacity is determined under different grid conditions and the distributed power configuration of the grid is optimized. At the same time, the DCNN model is used to analyze the power grid time series data and predict the voltage fluctuation of the power grid. The results are presented in graph form, showing trends in maximum capacity and voltage fluctuations under different grid conditions. Experimental results show that the overall model achieves satisfactory results in distributed power capacity optimization and voltage fluctuation prediction. Performance evaluation and comparison highlight the comprehensive model’s excellent performance in terms of prediction accuracy and computational efficiency, providing new possibilities for efficient management and reliable operation of power systems. The successful development of the model provides practical and reliable solutions for the future development of power systems.
Responses of fungal diversity and community composition after 42 years of prescribed fire frequencies in semi-arid savanna rangelands
Authors: Poswa Sanele Briged , Manyevere Alen , Mashamaite Chuene Victor
Published: February 2024

Prescribed fire frequencies have been widely used to reduce the risk of severe wildfire occurrences. In addition, several studies have been conducted to assess the impact of fire frequencies on vegetation, vertebrate, and invertebrate species, as well as soil physical and chemical properties. However, there is a lack of empirically based knowledge concerning the impact of fire frequency on soil microorganisms. This study assessed the effect of different fire frequencies on the diversity and composition of soil fungal communities in a semi-arid savanna rangeland. Soil samples were collected from an ongoing long-term trial at the University of Fort Hare (South Africa) on the following treatments: (i) no burning; (ii) annual burning (burned once every year); (iii) biennial burning (burned once every 2 years); (iv) triennial burning (burned once every 3 years); (v) quadrennial burning (burned once every 4 years); and (vi) sexennial burning (burned once every 6 years). Fungi were identified using high-throughput sequencing, with Shannon-Wiener and Inverse Simpson diversity indexes being used for diversity and network analysis. Principal coordinate analysis was used for Bray-Curtis distance matrices to visualise the relationships between treatments. The highest diversity was found in biennial burning, which was significantly different (p < 0.05) from the sexennial, quadrennial, and no burning treatments but was not different from the triennial and annual burning treatments. Regarding the taxa, Ascomycota and Basidiomycota were the phyla with the highest relative abundance, followed by Mortierellomycota, Chytridiomycota, and Rozellomycota. The different fire frequencies had an influence on soil fungi diversity and taxonomic composition in semi-arid savanna rangelands.
Industrial agglomeration, international R&D capital and air pollution-based on provincial spatial panel data
Authors: Gao Dawei , Deng Yan , Chen Zhengyang
Published: February 2024

Introduction: This paper presents a spatial effect model to examine the impact of industrial agglomeration and international R&D capital technology spillover on air pollution.

Methods: The study utilizes spatial panel data from 30 provinces and cities in China spanning the period 2008 to 2022, allowing for the decomposition of both direct and indirect spillover effects.

Results: Additionally, the research investigates the threshold effect of industrial agglomeration and international research and development capital and technology spillover on air pollution, considering independent research and development as the threshold variable. The findings reveal evident spatial autocorrelation and spatial dependence between industrial agglomeration, international R&D capital, and air pollution. Importantly, both international R&D capital technology spillover and industrial agglomeration exhibit a single threshold effect. This implies that surpassing the threshold value leads to a more pronounced positive impact of international R&D capital technology spillover on reducing air pollution, while the impact of industrial agglomeration on air pollution tends to be positive.

Discussion: The findings of this study have significant theoretical and practical implications for the promotion of sustainable economic development, particularly in China.
Longitudinal rheumatoid factor autoantibody responses after SARS-CoV-2 vaccination or infection
Authors: Keijzer Sofie , Oskam Nienke , Ooijevaar-de Heer Pleuni , Steenhuis Maurice , Keijser Jim B.D. , Wieske Luuk , van Dam Koos P.J. , Stalman Eileen W. , Kummer Laura Y.L. , Boekel Laura , Kuijpers Taco W. , ten Brinke Anja , van Ham S. Marieke , Eftimov Filip , Tas Sander W. , Wolbink Gerrit J. , Rispens Theo
Published: February 2024

Background

Rheumatoid factors (RFs) are autoantibodies that target the Fc region of IgG, and are found in patients with rheumatic diseases as well as in the healthy population. Many studies suggest that an immune trigger may (transiently) elicit RF responses. However, discrepancies between different studies make it difficult to determine if and to which degree RF reactivity can be triggered by vaccination or infection.

Objective

We quantitatively explored longitudinal RF responses after SARS-CoV-2 vaccination and infection in a well-defined, large cohort using a dual ELISA method that differentiates between true RF reactivity and background IgM reactivity. In addition, we reviewed existing literature on RF responses after vaccination and infection.

Methods

151 healthy participants and 30 RA patients were included to measure IgM-RF reactivity before and after SARS-CoV-2 vaccinations by ELISA. Additionally, IgM-RF responses after a SARS-CoV-2 breakthrough infection were studied in 51 healthy participants.

Results

Published prevalence studies in subjects after infection report up to 85% IgM-RF seropositivity. However, seroconversion studies (both infection and vaccination) report much lower incidences of 2-33%, with a trend of lower percentages observed in larger studies. In the current study, SARS-CoV-2 vaccination triggered low-level IgM-RF responses in 5.5% (8/151) of cases, of which 1.5% (2/151) with a level above 10 AU/mL. Breakthrough infection was accompanied by development of an IgM-RF response in 2% (1/51) of cases.

Conclusion

Our study indicates that de novo RF induction following vaccination or infection is an uncommon event, which does not lead to RF epitope spreading.
TIM-3, LAG-3, or 2B4 gene disruptions increase the anti-tumor response of engineered T cells
Authors: Cianciotti Beatrice Claudia , Magnani Zulma Irene , Ugolini Alessia , Camisa Barbara , Merelli Ivan , Vavassori Valentina , Potenza Alessia , Imparato Antonio , Manfredi Francesco , Abbati Danilo , Perani Laura , Spinelli Antonello , Shifrut Eric , Ciceri Fabio , Vago Luca , Di Micco Raffaella , Naldini Luigi , Genovese Pietro , Ruggiero Eliana , Bonini Chiara
Published: February 2024

Background

In adoptive T cell therapy, the long term therapeutic benefits in patients treated with engineered tumor specific T cells are limited by the lack of long term persistence of the infused cellular products and by the immunosuppressive mechanisms active in the tumor microenvironment. Exhausted T cells infiltrating the tumor are characterized by loss of effector functions triggered by multiple inhibitory receptors (IRs). In patients, IR blockade reverts T cell exhaustion but has low selectivity, potentially unleashing autoreactive clones and resulting in clinical autoimmune side effects. Furthermore, loss of long term protective immunity in cell therapy has been ascribed to the effector memory phenotype of the infused cells.

Methods

We simultaneously redirected T cell specificity towards the NY-ESO-1 antigen via TCR gene editing (TCRED) and permanently disrupted LAG3, TIM-3 or 2B4 genes (IRKO) via CRISPR/Cas9 in a protocol to expand early differentiated long-living memory stem T cells. The effector functions of the TCRED-IRKO and IR competent (TCRED-IRCOMP) cells were tested in short-term co-culture assays and under a chronic stimulation setting in vitro. Finally, the therapeutic efficacy of the developed cellular products were evaluated in multiple myeloma xenograft models.

Results

We show that upon chronic stimulation, TCRED-IRKO cells are superior to TCRED-IRCOMP cells in resisting functional exhaustion through different mechanisms and efficiently eliminate cancer cells upon tumor re-challenge in vivo. Our data indicate that TIM-3 and 2B4-disruption preserve T-cell degranulation capacity, while LAG-3 disruption prevents the upregulation of additional inhibitory receptors in T cells.

Conclusion

These results highlight that TIM-3, LAG-3, and 2B4 disruptions increase the therapeutic benefit of tumor specific cellular products and suggest distinct, non-redundant roles for IRs in anti-tumor responses.
The IFIH1-A946T risk variant promotes diabetes in a sex-dependent manner
Authors: Stock Amanda J. , Gonzalez Paredes Pierina , de Almeida Luciana Previato , Kosanke Stanley D. , Chetlur Srinivaas , Budde Hannah , Wakenight Paul , Zwingman Theresa A. , Rosen Aaron B.I. , Allenspach Eric J. , Millen Kathleen J. , Buckner Jane H. , Rawlings David J. , Gorman Jacquelyn A.
Published: February 2024

Type 1 diabetes (T1D) is an autoimmune disease in which pancreatic islet β-cells are attacked by the immune system, resulting in insulin deficiency and hyperglycemia. One of the top non-synonymous single-nucleotide polymorphisms (SNP) associated with T1D is in the interferon-induced helicase C domain-containing protein 1 (IFIH1), which encodes an anti-viral cytosolic RNA sensor. This SNP results in an alanine to threonine substitution at amino acid 946 (IFIH1A946T) and confers an increased risk for several autoimmune diseases, including T1D. We hypothesized that the IFIH1A946T risk variant, (IFIH1R) would promote T1D pathogenesis by stimulating type I interferon (IFN I) signaling leading to immune cell alterations. To test this, we developed Ifih1R knock-in mice on the non-obese diabetic (NOD) mouse background, a spontaneous T1D model. Our results revealed a modest increase in diabetes incidence and insulitis in Ifih1R compared to non-risk Ifih1 (Ifih1NR) mice and a significant acceleration of diabetes onset in Ifih1R females. Ifih1R mice exhibited a significantly enhanced interferon stimulated gene (ISG) signature compared to Ifih1NR, indicative of increased IFN I signaling. Ifih1R mice exhibited an increased frequency of plasma cells as well as tissue-dependent changes in the frequency and activation of CD8+ T cells. Our results indicate that IFIH1R may contribute to T1D pathogenesis by altering the frequency and activation of immune cells. These findings advance our knowledge on the connection between the rs1990760 variant and T1D. Further, these data are the first to demonstrate effects of Ifih1R in NOD mice, which will be important to consider for the development of therapeutics for T1D.
Case report: A case of advanced gastric cancer with multiple skin metastases, with significant relief from immunotherapy
Authors: Hao Wen , Chang Ruimin , Liu Jingxin , Wang Yibing , Ren Meijin , Xin Kai , Liu Baorui , Xie Jiaqi , Yang Yang
Published: February 2024

Gastric cancer is the fifth leading cause of cancer-related mortality worldwide, with a low 5-year survival rate in advanced stages. Cutaneous metastasis is rare in gastric cancer, with only 0.8-1% incidence. We reported a rare case of female gastric cancer. The patient had undergone subtotal gastrectomy and chemotherapy 13 years ago, followed by a subsequent surgery of residual stomach, partial jejunum, and partial colon resection 11 years later. The pathological examination revealed poorly differentiated stomach adenocarcinoma, Lauren classification: diffuse type. The patient received 2 cycles of SOX chemotherapy. Two years later, cauliflower-like skin nodules, which were surgically excised, appeared on the back. The histopathological examination showed a spindle cell tumor; no specific anti-tumor treatment was administered. Six months later, the skin lesions increased in size and number, spreading to the neck, chest, and abdomen, presenting as erythematous patches with some cauliflower-like elevations. A skin biopsy of a 1cm0.5cm0.3cm lesion on the left abdomen was performed, and based on the immunohistochemistry, clinical history, and the possibility of metastatic or infiltrating adenocarcinoma, the gastrointestinal origin was highly suspected. Genetic testing was performed on the gastric recurrence and skin lesions, revealing 103 shared genetic variations, further suggesting the skin metastasis originated from gastric cancer. Subsequently, the patient received 10 cycles of immunotherapy combined with intravenous chemotherapy (200mg Tislelizumab and 100mg albumin-bound paclitaxel). The treatment response was evaluated as partial remission, with significant improvement in the skin lesions compared to before. This case highlights the possibility of tumor metastasis in patients with extensive skin lesions in advanced gastric cancer. Early examination, diagnosis, skin biopsy, immunohistochemistry, and genetic sequencing are recommended.
Mangrove community-based management in Eastern Africa: experiences from rural Mozambique
Authors: Macamo Célia da Conceição Felisberto , Inácio da Costa Fátima , Bandeira Salomao , Adams Janine Barbara , Balidy Henriques Jacinto
Published: February 2024

Introduction

This study investigated a community-based management model in a mangrove-dependent community in central Mozambique and its adequacy as a management tool for conservation.

Methods

Satellite images were used to map changes in mangrove cover between 1996 and 2017. Individual interviews and Focus Group Discussions were conducted with community members to understand the mangrove restoration processes and management models.

Results and discussion

After unsustainable exploitation that led to mangrove deforestation, the local community engaged in a restoration and management program that started in the late 1990s. Local norms were delineated by the Natural Resources Management Committee (NRMC) aiming at (1) reducing extractive uses by introducing alternative income generating activities; (2) awareness and mangrove planting and (3) law enforcement. Ten hectares of forest were rehabilitated. However, the management system fails to exclude illegal cutters and to enforce regulatory and sanctioning mechanisms, due to resource limitations. More involvement from government authorities and other stakeholders is needed to enhance law enforcement and explore opportunities for carbon trading, tourism and payment for ecosystem services. Additional recommendations are to develop a community management plan and create alternatives to mangrove products and income. Mangrove community-based management is increasingly advocated in many developing countries to promote sustainable utilization of resources and conservation. Understanding the reasons behind the limited success and lessons learnt at this site will guide similar programs elsewhere in Mozambique and other parts of the globe.
Radar absorbing properties of different size carbon nanotube reinforced polymer composites
Authors: Yilmaz Atay Gül , Bilgiç Nurşah
Published: February 2024

Carbon nanotubes (CNTs), which have a diameter on the nanometer scale, are tube-like materials composed of carbon and graphite sheets. Carbon nanotubes play an important role in many fields such as nanotechnology, electronics, optics and material science due to their superior properties such as high elasticity, high thermal conductivity, low density and being more chemically inert. In this study, the use of carbon nanotubes in radar absorbing materials (RAM) to reduce the reflection of electromagnetic waves was investigated. In this sense, by using a polymeric composite structure reinforced with carbon nanotubes, a material with unique properties such as solid absorption, low weight/thickness and cost-effectiveness has been produced, minimizing the reflection of electromagnetic waves. For this purpose, carbon nanotubes with different particle sizes of 8 nm, 30–50 nm and 48–78 nm were blended with polyester at the ratio of 1%. The resulting composites were characterized using a Network analyzer in the frequency range of 8–12 GHz. According to the results obtained, it was observed that radar absorption increased with increasing particle size. It has been determined that by changing the particle sizes of carbon nanotubes in composites, the absorption bands can be adjusted to suit different applications in different frequency bands.
Application of dental pulp stem cells for bone regeneration
Authors: Liu Ye , Xiong Wei , Li Junyi , Feng Huixian , Jing Shuili , Liu Yonghao , Zhou Heng , Li Duan , Fu Dehao , Xu Chun , He Yan , Ye Qingsong
Published: February 2024

Bone defects resulting from severe trauma, tumors, inflammation, and other factors are increasingly prevalent. Stem cell-based therapies have emerged as a promising alternative. Dental pulp stem cells (DPSCs), sourced from dental pulp, have garnered significant attention owing to their ready accessibility and minimal collection-associated risks. Ongoing investigations into DPSCs have revealed their potential to undergo osteogenic differentiation and their capacity to secrete a diverse array of ontogenetic components, such as extracellular vesicles and cell lysates. This comprehensive review article aims to provide an in-depth analysis of DPSCs and their secretory components, emphasizing extraction techniques and utilization while elucidating the intricate mechanisms governing bone regeneration. Furthermore, we explore the merits and demerits of cell and cell-free therapeutic modalities, as well as discuss the potential prospects, opportunities, and inherent challenges associated with DPSC therapy and cell-free therapies in the context of bone regeneration.
Insights into CO oxidation on Au/TiO2-HMor zeolite catalysts at low temperature
Authors: Camposeco R. , Sánchez-Flores N. A. , Zanella R.
Published: February 2024

The effect of combining TiO2 and mordenite zeolite (HMOR), employed as support of gold nanoparticles, on the CO oxidation reaction at low temperature is studied. The amount of TiO2 encapsulated into HMOR was varied and the catalyst efficiency was investigated. The deposition-precipitation with urea (DPU) method was used to deposit gold nanoparticles; likewise, the synthesis of monometallic catalysts based on TiO2 and HMOR is reported. The synthesized materials were characterized by X-ray diffraction (XRD), nitrogen adsorption, X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). The addition of TiO2 influenced the properties of the TiO2-HMOR composite, and its catalytic performance in the CO oxidation from 20°C. It was established that the 5Au/(28)TiO2-HMOR composite was the most active catalyst at lower temperatures, which was ascribed to the close contact among the components of the TiO2-HMOR composite, gold dispersion, gold and TiO2 loadings, and Au and Ti species present in the catalysts.
Clinical and imaging markers for the prognosis of acute ischemic stroke
Authors: Pei Chenyang , He Che , Li Han , Li Xiangying , Huang Weihui , Liu Jun , Yin Jianzhong
Published: February 2024

Background and purpose

Significant differences in the outcomes observed in patients with acute ischemic stroke (AIS) have led to research investigations for identifying the predictors. In this retrospective study, we aimed to investigate the relationship of different clinical and imaging factors with the prognosis of AIS.

Materials and methods

All clinical and imaging metrics were compared between the good and poor prognosis groups according to the modified Rankin Scale (mRS) score at 90 days after discharge. Clinical factors included gender, age, NIHSS scores at admission, and other medical history risk factors. Imaging markers included the lesion’s size and location, diffusion, and perfusion metrics of infarction core and peripheral regions, and the state of collateral circulation. Spearman’s correlations were analyzed for age and imaging markers between the different groups. The Chi-square test and Cramer’s V coefficient analysis were performed for gender, collateral circulation status, NIHSS score, and other stroke risk factors.

Results

A total of 89 patients with AIS were divided into the good (mRS score ≤ 2) and poor prognosis groups (mRS score ≥ 3). There were differences in NIHSS score at the admission; relative MK (rMK), relative MD (rMD), relative CBF (rCBF) of the infarction core; relative mean transit time (rMTT), relative time to peak (rTTP), and relative CBF (rCBF) of peripheral regions; and collateral circulation status between the two groups (p < 0.05). Among them, the rMK of infarction lesions had the strongest correlation with the mRS score at 90 days after discharge (r = 0.545, p < 0.001).

Conclusion

Perfusion and diffusion metrics could reflect the microstructure and blood flow characteristics of the lesion, which were the key factors for the salvage ability and prognosis of the infarction tissue. The characteristics of the infarction core and peripheral regions have different effects on the outcomes. Diffusion of infarction core has strong relations with the prognosis, whereas the time metrics (MTT, TTP) were more important for peripheral regions. MK had a more significant association with prognosis than MD. These factors were the primary markers influencing the prognosis of cerebral infarction patients.