bluuebunny/crossref_metadata_2025 huggingface dataset:
Periodontal Status and Gingival Conditions of the Tea Workers
Authors: Bhowmick Anjan , Nurunnabi Mohammad , Adhikary Laboni , Rahman Md Shamsur , Saha Tanmoy , Hossain Kazi Jahangir
Published: June 2023

Background: Periodontitis and gingivitis are prevalent chronic inflammatory diseases. The examination of the periodontal and gingival conditions is complex and requires a proficient dental healthcare provider to detect the patient’s definite problems.

Methodology: The study was designed as a cross-sectional study to assess the periodontal status and gingival conditions of the 372 tea workers in the purposively selected Premnagar tea garden located in Moulvibazar district, Sylhet 3200, Bangladesh. ‘Community Periodontal Index’ (CPI) was used to measure the periodontal status.

Results: Most of the workers (38.2%) were from the age group ≥37 years and illiterates (62.1%). More than two-fourths (57.8%) had detected calculus during probing, one-fourth (25.3%) had a shallow pocket and only 11.6% had healthy periodontal status. The prevalence of poor periodontal status was 88.4%. Among the dental cleaning aids such as toothbrush users, 15.6% had healthy periodontal status, whereas the smokeless tobacco non-users (22.6%) and pan-supari non-chewers (75.0%) had healthy periodontal conditions. The level of periodontal status factors was statistically significant with age, educational qualification, and monthly household income (p<0.05) of the workers.

Conclusion: The study warrants poor periodontal health status among the tea workers. It is essential to do the periodical assessment of the periodontal condition, risk factors analysis, and management complications by establishing dental health corners in tea garden health settings.

Eastern Med Coll J. Jan 2023; 8 (1) 19-23
Factors Affecting Interest In Using E-Commerce And E-Wallet With Using Technology Acceptance Model
Authors: Fitranita Vika , Zoraya Intan , Wijayanti Indah Oktari
Published: June 2023

This study aims to determine the Influence of Interest in Using an E-Commerce-Based Accounting Information System and the interest of OVO customers using the Technology Acceptance Model (TAM). Data collection was carried out by giving questionnaires to respondents using OVO and Tokopedia in Bengkulu City. The sample in this study were 215 respondents using OVO and Tokopedia. Measurement of data in this study using Multiple Regression analysis with analysis tools using data processing software Eviews 12. In this study used data quality test, classical assumption test (normality test, multicollinearity test, and heteroscedasticity test), multiple linear regression analysis and hypothesis testing. The results of the study show that the ease of use variable has no effect on interest in use, the variables perceived usefulness, trust, attitudes towards use are significantly positive on interest in use, risk variables have a significant negative effect on interest in use
Metode Simple Additive Weighting Untuk Pemilihan Penerima Bantuan Program Raskin
Authors: Setiyowati  , Sri Siswanti  , Alvareza Anggada Tama  , Andriani Kusumaningrum
Published: June 2023

Program Raskin merupakan salah satu program pemerintah untuk membantu masyarakat miskin dan rawan pangan. Tidak semua warga masyarakat berhak mendapatkan Program Raskin, tetapi hanya warga yang tergolong kelurga miskin dan rawan pangan. Program Raskin berupa pemberian bantuan beras yang diberikan setiap triwulan (tiga bulan) sebanyak 15kg/bulan, selama 12 bulan. Kriteria masyarakat yang memperoleh bantuan program raskin berdasarkan pada penghasilan perbulan, jumlah tanggungan orang dalam keluarga, dan kondisi rumah. bantuan program raskin belum merata dalam pembagiannya, karena terkendala kuota. Sehingga diperlukan perangkingan untuk menentukan penerima Program Raskin, serta mengembangkan aplikasi untuk pemilihan penerima bantuan program Raskin. Perangkingan menggunakan sistem pengambilan keputusan dengan metode Simple Additive Weighting (SAW) di perlukan untuk membantu dalam pemilihan warga yang berhak menerima bantuan Program Raskin. Metode SAW mampu menentukan bobot untuk setiap kriteria, dan melakukan perankingan untuk menyeleksi alternatif terbaik dari sejumlah alternative, kriteria yang digunakan penghasilan, jumlah anak, kondisi rumah. Pengembangan sistem dengan menggunakan model Waterfall, pada tahap desain menggunakan pendekatan terstruktur. Hasil akhir berupa keputusan penerima Program Raskin, serta sebuah Aplikasi yang dapat dipergunakan untuk pengambilan keputusan pemilihan warga yang berhak mendapatkan bantuan Program Raskin. Pengujian sistem dengan menggunkan uji fungsionalitas (Black Box). Hasil uji sistem menunjukkan bahwa sistem berfungsi dengan dengan baik sesuai yang diharapkan. Uji validitas dilakukan untuk menguji hasil perhitungan Metode SAW dengan membandingkan yang menghasilkan uji sistem dinyatakan valid 100%.
PENGARUH PELAYANAN CUSTOMER SERVICE TERHADAP KEPUASAN NASABAH BANK BRI CABANG BANJARMASIN AHMAD YANI
Authors: Widiati Widiati
Published: June 2023

Hasil penelitian menunjukkan bahwa Pelayanan customer service yang diberikan oleh Bank BRI Cabang Banjarmasin Ahmad Yani sudah optimal. Berdasarkan olah data pelayanan customer service dari 10 indikator mayoritas responden menyatakan baik pada aspek kemampuan dengan rata-rata tertinggi sebesar 5,60, Kepuasan nasabah, berdasarkan olah data variabel kepuasan nasabah dari 3 variabel mayoritas responden menyatakan sangat puas, terutama dalam hal kepuasan pada aspek harapan dan keseluruhan dengan memiliki nilai rata-rata tertinggi sebesar 5,70. Hasil penelitian ini membuktikan bahwa terdapat pengaruh pelayanan customer service terhadap kepuasan nasabah. Dengan demikian hipotesis penelitian yang berbunyi “Ada Pengaruh antara Pelayanan Customer Service terhadap Kepuasan Nasabah pada Bank BRI Cabang Banjarmasin Ahmad Yani” diterima.
The Investigation of Relationship between Functional Mobility Levels of Children with Chronic Disability and Caregivers’ Quality of Life
Authors: Ahmadov Ramil , Demir Nazlı , Kurt Merve , Tarsuslu Tülay
Published: June 2023

Purpose: This study aims to look at the relationship between carers' quality of life and the functional mobility levels of children with chronic disabilities.Methods: The study included 30 children (22 boys, 8 girls) with chronic disabilities and their caregivers. The socio-demographic characteristics of the children and caregivers were collected.Caregivers' quality of life was evaluated with the Adult Carer Quality of Life Questionnaire (AC-QoL), and children’s functional mobility was evaluated with the mobility subscale of the Pediatric Evaluation Of Disability Inventory (PEDI-Mobility). Results: The age of the children included in the study was 7.0 (5.0-12.0) years and the age of their caregivers was 38.00 (32.75-41.25) years. The AC-QoL score of caregivers was 85.00 (72.00-93.50) out of a total of 120 points, and the PEDI-Mobility score was 33.00 (3.75-53.00) out of a total of 58 points. In the correlation analysis, no significant correlation was found between the PEDI-Mobility total score and the AC-QoL total score (p>0.05).However, there was a negative correlation between the PEDI-Mobility total score and the AC-QoL “ability to care” subscale score(r= -0.403; p=0.027). Conclusion: According to the results of the study, no relationship was found between the functional levels of children and the quality of life of their caregivers. Studies with a larger sample size and consideration of aspects including sociocultural level, economic considerations, and health services that may affect the quality of life of families are regarded to be necessary in order to achieve more conclusive findings on this subject.
Desafios para prevenção do câncer de próstata em mulheres transgêneras
Authors: Carvalho Matheus dos Santos , Silva Pâmela Tays de Holanda , Silva Polyana Maccoy e , Valgueiro Natália de Carvalho Lefosse , Bernardino Amanda de Oliveira , Souza Karla Romana Ferreira de
Published: June 2023

O estudo objetivou a descrever os desafios para prevenção do câncer de próstata em mulheres transgêneras. Para a elaboração deste trabalho, utilizou-se uma revisão narrativa da literatura que analisou estudos científicos nacionais e internacionais, durante o período de 2015 a 2021. Considerando o objeto do estudo, inicialmente os artigos referentes à temática abordada foram pesquisados no banco de dados da Scientific Electronic Library Online, Literatura Latino-Americana e do Caribe em Ciências da Saúde, U. S. National Library of Medicine (PubMed) e Medical Literature Analysis and Retrieval System Online. Desse modo, utilizou-se os seguintes descritores combinado com o operador booleano AND: pessoas transgênero, neoplasias da próstata e prevenção primária. A revisão foi dividida em câncer de próstata em mulheres transgêneras e os desafios para prevenção do câncer de próstata em mulheres transgêneras. Por fim, faz-se necessário entender a epidemiologia do câncer de próstata e as necessidades específicas de prevenção na população transgênera e que se adote políticas públicas de saúde que inclua esse público na estratégia de prevenção.
Climatério: papel do enfermeiro da atenção primária à saúde no cuidado e orientação da sexualidade: uma revisão integrativa de literatura
Authors: Ferreira Daniela Alves de Souza , Freitas Eduarda Medeiros de , Cortez Eduardo Nogueira , Alves Larissa Luisa Costa
Published: June 2023

Este estudo objetivou-se em realizar uma revisão integrativa de literatura para explorar o papel do enfermeiro da atenção primária à saúde no cuidado e orientação da sexualidade durante o climatério. A metodologia utilizada consistiu em busca e análise de estudos científicos em bases de dados eletrônicas, periódicos científicos e teses/dissertações relevantes ao tema. Foram incluídos estudos que abordavam a atuação do enfermeiro na orientação e cuidado da sexualidade durante o climatério, bem como os resultados obtidos. Os resultados da revisão demonstraram que o enfermeiro desempenha um papel importante nessa fase da vida da mulher. A atuação abrange desde a oferta de informações claras e atualizadas sobre as alterações físicas e psicológicas do climatério, até o suporte emocional e a orientação sobre práticas sexuais saudáveis e seguras. Verificou-se que o enfermeiro tem a capacidade de estabelecer uma relação de confiança e empatia com as mulheres em climatério, o que possibilita a abertura para discussões sobre questões íntimas e sensíveis relacionadas à sexualidade. Como conclusão, este estudo evidencia a importância do enfermeiro da Atenção Primária à Saúde no cuidado e orientação da sexualidade durante o climatério. O enfermeiro deve estar preparado para abordar tais questões de forma sensível e acolhedora, fornecendo informações adequadas e promovendo a saúde sexual e o autocuidado das mulheres em climatério. Essa abordagem integral contribui para uma melhor qualidade de vida e bem-estar das mulheres nessa fase da vida.
THE ROLE OF IMPULSIVITY IN MILITARY LEADERSHIP - A LITERATURE REVIEW
Authors: BUREŠOVÁ IVA , ZEMAN JÁCHYM , KNAP-STEFANIUK AGNIESZKA , ROLENEC OTA , SVOBODA IVO
Published: June 2023

This paper focuses on impulsivity as a significant predictor of risk-taking and decision-making in the context of military leadership. It examines the possible sources and implications of elevated levels of impulsivity in this specific leadership domain, looks at the approaches to studying this subject, as well as other key implications of this construct. Attention is also paid to the prevention and elimination of undesirable forms of impulsive behavior, especially in the context of dealing with challenging situations in the real conditions of professional activity of military leadership students and in the performance of military service of military leaders.
Screening of Immunostimulatory Activity from Indonesian Kampung (Gallus domesticus) Egg White Water Extract: in vitro study
Authors: Mulyani Rizka , Harmayani Eni , Nurliyani  , Ishida Momoko , Nishi Kosuke , Sugahara Takuya
Published: June 2023

The most popular Indonesian native chicken, known as kampung chicken, is maintained under free-range conditions therefore it is prone to high environmental stress. Indonesian native chicken and its product are always regarded as having health benefits compared to commercial chicken by Indonesian society. But there is still limited report regarding Indonesian native chicken and its product. This study focused on screening immunostimulatory activity from Indonesian native chicken egg white using in vitro approaches as a functional food. Indonesian native chicken egg white (NEW) was extracted using distilled water supplemented to human-human hybridoma HB4C5 cells to examine the IgM production stimulating activity using ELISA. The gene expression was also examined using qRT-PCR. The ability of NEW on stimulating immunoglobulin production by mouse splenocytes was analyzed. Commercial egg white water extract (CEW) was used as a comparison. The data were analyzed using One-way ANOVA and continued by using post-hoc analysis using Tukey’s multiple comparison test. The results showed that NEW and CEW modulated IgM production by the HB4C5 cells 8.72-fold and 6.75-fold, respectively, compared to control. NEW stimulated immunoglobulin (Ig) production by the mouse splenocytes higher than CEW. To conclude, NEW provides an immunostimulating activity that can potentially act as a health-promoting food.
A Review on the Health Benefits of Kalakai (Stenochlaena Palustris)
Authors: Pandiangan Febbyandi Isnanda , Oslo Edrick Alvaro , Destine Fiona , Josephine Josephine , Anwar Rania Nadira
Published: June 2023

Kalakai (Stenochlaena palustris) is a common plant in Kalimantan and Sumatra which has been consumed locally as a vegetable. Information about the benefits of kalakai as a functional food has spread widely in the community. However, scientific information and research investigating the potency of kalakai are scarce, and the scientific literature is dominated by local Indonesian-language journals. This review aims to explore and compile the potential health benefits of kalakai based on its nutritional and bioactive content. Kalakai is reported to have various health benefits such as high fibre, antianemia, antioxidant, antidiabetic and antimicrobial activity. Referring to the current status of research, the processing of kalakai as a food functional or nutraceutical ingredient for antioxidative function and controlling blood sugar are the most potential. However, clinical and safety trials still need to be carried out as part of the preparation for the use of kalakai as a functional and nutraceutical food ingredient in the near future.
Chunso Shin Choi's Study on 11 Won(十一原)
Authors:   The Korean Society of Culture and Convergence, Kim Suhyun
Published: June 2023

The purpose of this paper is to discuss 11Won(十一原) of Shin Choi, a writer in the mid-Joseon period. As a leading figure in writing in the mid-Joseon Dynasty, Shin Choi accepted various literary theories of China. 11Won(十一原) is an article expressing opinions in the style of circle on 11 topics across society. Won(原) is a style of writing that emphasizes the legitimacy of the author's claim through the process of 'chubonsowon (推本所原)', which traces the root in the process of introduction. As for the research method, after examining the origin of the original style and the situation of the Joseon literary world, 11Won(十一原) was analyzed. By setting three categories: customs and people's livelihood, politics centered on courtesy and music, and recruitment of talented people, the content and form were investigated. Each of the 11 writings has completeness and is organically connected, reflecting a high level of planning power. The sentences are very systematic and restrained, so the level of prose in the mid-Joseon period can be judged.
A Study on the Innovation Cases and Improvements of University Education
Authors:   The Korean Society of Culture and Convergence, Jeon Jonghee
Published: June 2023

The purpose of this paper is to explore recent innovation cases and improvements related to university education. Universities in the current era are making every effort to quickly prepare and implement self-rescue improvements to fulfill their unique role and meet the trend of the times due to population decline and rapid technological development. In this study, innovation trends in domestic and foreign university education, which were investigated mainly in April-May 2023, were confirmed and related contents were presented. Recently, attempts and methods for university education innovation at home and abroad were examined, and educational discussions and improvements were sought to the extent practically possible. As a result of a comprehensively examining the contents of the research topic, it was confirmed that, in particular, in university education innovation, integrated selection, convergence education, industry-academia cooperation, flexible curriculum, building infrastructure, and learner-led in-depth learning induction methods and activities are appearing. Through this study, it is expected that we will be able to start in-depth discussions on the innovation of university education that practices learner-centered education while flexibly responding to the needs of the rapidly changing era.
PROTECTION OF CHILDREN'S RIGHTS AND WOMEN'S RIGHTS AS PART OF HUMAN RIGHTS IN INDONESIA THROUGH RATIFICATION OF INTERNATIONAL REGULATIONS
Authors: Failin Failin , Yuserlina Anny , Ibrahim Eviandi
Published: June 2023

Protection of human rights, regardless of age and gender, everyone has the right to protection. Indonesia as a state of law is obliged to protect the human rights of its people, and joining Indonesia as a member of the United Nations requires it to ratify regulations relating to the protection of children and women. This article will discuss the extent of ratification by the Government of Indonesia to promote the protection of children's rights and women's rights. This type of legal research is also commonly referred to as doctrinal legal research or library research. It is called doctrinal legal research because this research is only aimed at written regulations so that this research is very closely related to libraries because it will require secondary data in the library. The results of the study show that the Government of Indonesia has advanced the protection of children's rights with the ratification of the Convention on the Rights of the Child in the Presidential Decree of the Republic of Indonesia Number 36 of 1990, besides that it also gave birth to the Child Protection Law. As for the protection of women, in 1984 Indonesia ratified the Convention on the Elimination of All Forms of Discrimination Against Women through Law Number 7 of 1984 concerning Ratification of the Convention on the Elimination of All Forms of Discrimination Against Women, which was then followed by Presidential Instruction Number 9 of 2000 concerning Gender Mainstreaming and Law Number 23 of 2004 concerning the Elimination of Domestic Violence, so that the protection of women in Indonesia is more protected.
CRITICISM ANALYSIS OF THE EFFECTIVENESS OF INDONESIA'S ECONOMIC CRIMINAL POLICY IN THE PERSPECTIVE OF ISLAMIC LAW
Authors: Firdaus Muhammad Irkham
Published: June 2023

Economic crime rates tend to be high and generate large losses every year, because one of the biggest factors is the ineffective economic crime policy in providing deterrent and firmness to the perpetrators. Economic crime policies play an important role in creating public welfare, if the economic crime rate is high, it means that the welfare of the community has not been created optimally, so there needs to be efforts to review and fix these criminal policies. This research aims to determine the effectiveness of Indonesia's criminal policy in the perspective of Islamic law, as well as to criticize the economic criminal policy in Indonesia. This research explores data sources from various data on the facts of crimes that occur in Indonesia, which are then analyzed by qualitative methods from the point of view of Islamic law. The results of this research show that economic crime policies have not been effective in tackling economic crimes, because they do not provide a deterrent effect for perpetrators of economic crimes. Whereas in Islam, the prevention of economic crime is divided into two categories, namely jarimah hudud and jarimah ta'zir. Sanctions applied in Islamic law are more of a deterrent effect, because sanctions for serious economic crimes have been determined directly by Allah SWT, then economic criminal policies in Indonesia tend not to achieve their essential goals, namely welfare and social protection for the community, this happens because there are shortcomings in the three stages of implementing economic criminal policies in Indonesia, namely the legislative stage, judicial stage, and executive stage. It is necessary to make a new formulation in the economic criminal policy in Indonesia, namely by specifying the sanctions that will be received by the perpetrators of criminal acts with the sanctions stated in Islamic law, from the point of view of the deterrent effect and the consequences received by the perpetrators.
JURIDICAL REVIEW LARGE-SCALE SOCIAL RESTRICTIONS IMPLEMENTED BY LOCAL GOVERMENTS
Authors: Nur Fasha Moch Gandi , Diamantina Amalia
Published: June 2023

This research discusses the policy guidelines for implementing PSBB, which is one of the models used by the Government in handling Covid-19. The focus is on the legal issues related to the process of determining PSBB at the regional level. The paper uses a normative juridical approach to examine the proposed PSBB policy by the Local Government and its legal implications. The research finds that the proposed PSBB policy has limited the Local Government's ability to set PSBB at the regional level. The legal implications of imposing large-scale social restrictions at the regional level have resulted in a failure to realize the legal benefits of treating the Covid-19 pandemic as an emergency. The direction and legal implications of the proposed PSBB policy are discussed in detail in this paper.
TRANSITION OF ONLINE SINGLE SUBMISSION (OSS 1.1 TO RISK-BASED APPROACH) TO INCREASE INVESTMENT IN WEST SUMATERA
Authors: Anggunsuri Upita , Zahara Zahara
Published: June 2023

The implementation of an online single submission risk-based approach (OSS RBA) is a manifestation of a government program to increase investment in a region. Business risk classification is an instrument of the Central Government and Local Government in controlling business activity. This study aims to find out the readiness of the local government to implement an online single submission risk-based approach. The method of the study is empirical legal research. The result of this study shows that Padang City has issued Mayor Regulation No 43 /2021 on Guidelines and Procedures of an online single submission risk-based approach Supervision as a follow-up to Government Regulation No. 5/2021 on implementation of online single submission risk-based approach; meanwhile, several other regions are in the process of drafting local regulations relate to implementing online single submission risk-based approach in West Sumatera Province. The challenges of local government to implement OSS RBA consists of indefinite license periods in the particular business field (Appendix I Government Regulation No 5/2021), the regional support system has not integrated OSS RBA and the readiness for the transition from manual RDTR to digital RDTR in West Sumatera.
Local art museums and visitors: audience and attendance development. Theoretical requirements and empirical evidence
Authors: Cerquetti Mara
Published: June 2023

During the 20th century museum visitor studies have used different approaches with varying results. They have covered not only individual demographic and psychographic characteristics, but also the motivation, needs and expectations of both visitors and non-visitors. According to the visitor-oriented approach, most research has suggested to attract and satisfy new audiences promoting social interaction and the emotional involvement of visitors through edutainment, new technological devices, exhibitions and other ‘block-buster’ events.

In this paper we will analyse the content and language of art museum communication, to verify if the general knowledge people have when they visit a museum is sufficient to understand and appreciate the complex value of exhibits in museums and if museum communication strategies are suitable to the mise en valeur of local cultural heritage. Analysing the findings of a local survey on the relationship between museums and their real and potential visitors, carried out among students, the following paper argues that museums and education should enhance the distinctive features of local cultural heritage, both to promote the understanding of its complex value and to reach new audiences.
Experiential Learning Theory applied to the Degree Profiles of the Arts and Cultural Management Programmes in Europe1
Authors: Kuznetsova-Bogdanovits Kristina
Published: June 2023

European Higher Education Area (EHEA) has been in construction for a bit more than a decade. This paper aims to explore this process as well as the communication of cultural and arts management degree programmes through outcome-based education vocabulary as something flagshipped by the EU. It also aims to engage into critical discussions about learning outcomes and experiential learning process. The theoretical framework of the paper is mainly based on constructivist education paradigm and, to a lesser extent, on connectivism; David Kolb’s experiential learning cycle and Benjamin Bloom’s taxonomy of learning domains are applied in the analysis. 23 degree programmes of ENCATC members are approached through the degree profiles provided on their websites: learning outcomes, profiles of the graduates, and other informative texts. We are questioning the connections between the learning process, learning outcomes and profiles of the graduates, and giving practical suggestions for the programmes administrators to make the information on the degree more clear and holistic, both for the students and for potential employers.
Intangible cultural heritage projects – National policies and strategies. The creation of intangible cultural heritage inventories
Authors: Konach Teodora
Published: June 2023

The UNESCO Convention for the Safeguarding of Intangible Cultural Heritage of 2003 has established a new, holistic approach to cultural heritage, as well as a new set of administrative and legal instruments and strategies for identifying, preserving, managing and promoting intangible cultural heritage. The policy of intangible culture entails the conceptualisation of the elements of intangible heritage in the national cultural policy framework. Administration strategies and methods are often confronted with scientific contextualisation and various policies of representation and identification. Thus, while articulating the idea of the growing importance of intangible cultural heritage, the national authorities increasingly construct the national inventories through processes of worldwide networking and positioning through symbolic meanings such as “national issues” and “national culture”. This paper presents a brief review of administrative and legal measures and policies concerning intangible cultural heritage of some selected countries.
Sevoflurane inhibited reproductive function in male mice by reducing oxidative phosphorylation through inducing iron deficiency
Authors: Zhang Xue , Zuo Yong , Zhang Jianhua , Zhang Di , Naeem Muhammad , Chang Yanzhong , Shi Zhenhua
Published: June 2023

Sevoflurane (Sev) is one of the commonly used inhalation anesthetic chemicals in clinics. It has great impact on spermatogenesis and fertilization in male animals. The underlying mechanism remains largely unexplored. Based on our previous research, we hypothesized that Sev induced iron metabolism disturbance in the testis and epididymis and inhibited the spermatogenesis. In this study, two-month-old C57BL/6 male mice were treated with 3% Sev for 6 h, and their fertility (including sperm concentration, sperm mobility, and the number of offspring) was evaluated. Mice testis, epididymis, and sperm were harvested and subjected to Western blot analysis and immunofluorescence analysis. Iron levels were reflected by the gene expression of iron metabolism-related proteins (including ferritin, TfR1, and FpN1) and ICP-MS and Perl’s iron staining. Electron transport and oxidative phosphorylation levels were measured by Oxygraph-2k and ATP contents. The activity of ribonucleotide reductase was evaluated by assay kit. DNA synthesis status in testis and/or epididymis was marked with BrdU. Cell proliferation was evaluated by double immunofluorescence staining of specific protein marker expression. Our results revealed that the mice exposed to Sev showed damaged testicular and epididymis structure and significantly reduced the sperm concentration, sperm motility, and fertility. Sev decreases the iron levels through down-regulating the expression of H-ferritin, L-ferritin, and FpN1, and up-regulating the expression of TfR1 in the testis and epididymis. Iron levels also significantly reduced in germ cells which decrease the number of germ cells, including sperm, Sertoli cells, and primary spermatocyte. Iron deficiency not only decreases electron transport, oxidative phosphorylation level, and ATP production but also suppresses the activity of ribonucleotide reductase and the expression of Ki67, DDX4, GATA1, and SCP3, indicating that Sev affects the spermatogenesis and development. Meanwhile, Sev impaired the blood-testis barrier by decreasing the ZO1 expression in the testis and epididymis. The damage effect induced by Sev can be significantly ameliorated by iron supplementation. In conclusion, our study illustrates a new mechanism by which Sev inhibits spermatogenesis and fertility through an oxidative phosphorylation pathway due to iron deficiency of epididymis and testis or sperm. Furthermore, the damaging effects could be ameliorated by iron supplementation.
A novel force transduction pathway from a tension sensor to the gate in the mechano-gating of MscL channel
Authors: Sawada Yasuyuki , Nomura Takeshi , Martinac Boris , Sokabe Masahiro
Published: June 2023

The bacterial mechanosensitive channel of large conductance MscL is activated exclusively by increased tension in the membrane bilayer. Despite many proposed models for MscL opening, its precise mechano-gating mechanism, particularly how the received force at the tension sensor transmits to the gate remains incomplete. Previous studies have shown that along with amphipathic N-terminus located near the cytoplasmic surface of the membrane, Phe78 residue near the outer surface also acts as a “tension sensor,” while Gly22 is a central constituent of the “hydrophobic gate.” Present study focused on elucidating the force transmission mechanism from the sensor Phe78 in the outer transmembrane helix (TM2) to the gate in the inner transmembrane helix (TM1) of MscL by applying the patch clamp and molecular dynamics (MD) simulations to the wild type MscL channel and its single mutants at the sensor (F78N), the gate (G22N) and their combination (G22N/F78N) double mutant. F78N MscL resulted in a severe loss-of-function, while G22N MscL caused a gain-of-function channel exhibiting spontaneous openings at the resting membrane tension. We initially speculated that the spontaneous opening in G22N mutant might occur without tension acting on Phe78 residue. To test this hypothesis, we examined the (G22N/F78N) double mutant, which unexpectedly exhibited neither spontaneous activity nor activity by a relatively high membrane tension. To understand the underlying mechanism, we conducted MD simulations and analyzed the force transduction pathway. Results showed that the mutation at the tension sensor (F78N) in TM2 caused decreased interaction of this residue not only with lipids, but also with a group of amino acids (Ile32-Leu36-Ile40) in the neighboring TM1 helix, which resulted in an inefficient force transmission to the gate-constituting amino acids on TM1. This change also induced a slight tilting of TM1 towards the membrane plane and decreased the size of the channel pore at the gate, which seems to be the major mechanism for the inhibition of spontaneous opening of the double mutant channel. More importantly, the newly identified interaction between the TM2 (Phe78) and adjacent TM1 (Ile32-Leu36-Ile40) helices seems to be an essential force transmitting mechanism for the stretch-dependent activation of MscL given that substitution of any one of these four amino acids with Asn resulted in severe loss-of-function MscL as reported in our previous work.
The Sedimentary records of the Hapcheon impact crater basin in Korea over the past 1.3 Ma
Authors: Lee Jin-Young , Shin Seungwon , Yoon Hyun Ho , Kim Jin Cheul , Choi Yire , Nahm Wook-Hyun , Kim Heejung
Published: June 2023

The Hapcheon impact crater is the only meteorite impact crater identified on the Korean peninsula. However, the morphology of the impact crater and the nature of the meteorite collision are unknown. In this study, we analyzed the sedimentary facies using grain size data; computed tomography images, 14C, 10Be, and optically stimulated luminescence dating on a >66-m-long sediment core (20HCL04) recovered from the Hapcheon Basin. Four sedimentary units and 10 types of facies were documented in the Hapcheon Basin sediment core. The sedimentary units comprise 1) a lower part (unit 1) that is dominated by moderately to well-sorted coarse gravel, which contains some impact-related sediments; 2) a middle part (units 2 and 3) dominated by well-laminated mud; and 3) an upper part (Unit 4) that is dominated by poorly sorted coarse gravel supplied from the surrounding mountain slopes by alluvial and fluvial processes. After the meteorite impact, the Hapcheon impact crater was filled with deposits from the crater wall after ca. 1.3 Ma and the Hapcheon Basin became a deep lake environment. After ca. 0.5 Ma, sediments were supplied from the surrounding mountains until the lake was filled. Finally, sediments were deposited in an alluvial fan setting. In addition, the Hapcheon Basin sedimentary cores contain a tephra layer and deformed soft sediments that can be used to investigate volcanic and seismic events on the Korean Peninsula over the past 1.3 Ma.
Wide field electromagnetic method calculation in arbitrary orientation and its effectiveness analysis
Authors: Tian Hongjun , Qiang Jianke , Li Kun , Tan Zhangkun , Zhang Ying , Zhu Dexiang
Published: June 2023

The wide field electromagnetic method E_Ex observation method requires the horizontal electrical field source (AB) to be parallel to the measure station (MN), but the complex terrain conditions make it difficult to meet the AB parallel MN in field construction, and there is always an azimuthal difference α between them, which will bring a large calculation error and cause distortion of the interpretation parameters if the calculation method E_Ex continues to be used. In order to tackle this problem, we analyse and study the E_Ex method and derive the new calculation method of E_EMN for the wide field electromagnetic method along the MN (E_EMN). The method is based on the intrinsic relationship between the electric field components Ex, Ey and the azimuthal difference α and derives the expression for the electric field E_EMN along the MN direction, and then uses the E_EMN to calculate the wide field apparent resistivity. In this paper, we design a three-layer geoelectric model and calculate the azimuthal angle difference α = 0° and α = 15°wide field apparent resistivity parameters, respectively. The results show that the relative error between the calculated apparent resistivity and the theoretical value is less than 1%, which verifies the correctness and validity of the method. To further verify the accuracy of the method, experimental work on azimuthal difference α was carried out next to a known well in Sichuan. The results show that: firstly, when α is 1°, 3°, 5°, 10°, and 15°, respectively, the relative error of each frequency point increases with α increasing. Secondly, when α is 1°, 3°, 5°, 10°, and 15°, respectively, the relative error of each frequency point is less than 10% when the EMN method is used to calculate the wide field apparent resistivity value; thirdly, taking α = 15° as an example, single-station inversions are performed with Ex and EMN apparent resistivity parameters, in which the Ex inversion results are more different from the trend of the logging curve, while the EMN inversion results are relatively more consistent with the trend of the logging curve. The arbitrary orientation EMN calculation method proposed in this paper can effectively reduce the influence of α on the interpretation parameters and improve the accuracy of interpretation, and also greatly expand the applicability and flexibility of Ex wide field electromagnetic method in the observation of complex terrain areas, which has important theoretical research and practical production significance.
Multi-objective reactive power optimization strategy of power system considering large-scale renewable integration
Authors: Ma Xiping , Liang Chen , Dong Xiaoyang , Li Yaxin
Published: June 2023

To fully tap the abilities of renewables in reactive power optimization, this paper develops a detailed model for the power regulation capabilities of wind turbines and photovoltaic units and studies their impact on the power system’s operation. First, the power system model with renewables integration is established using AC power flow. The wind turbines and photovoltaic units are modeled in detail according to their topologies and operating characteristics, and then further simplified according to the feasible region. An improved DC power flow model is adopted to handle the non-linear characteristics of the power system. On this basis, a multi-objective reactive power optimization model is constructed to minimize the power generation cost, wind and solar power curtailment, and voltage offset. Finally, comparisons between two types of models in different scenarios are designed. Numerical simulations demonstrate that the participation of renewables in reactive power regulation can improve the operational economy and voltage stability of power systems.
Precipitation-type discrimination and changes in related climate indices in the upper Heihe River Basin during 1960–2021
Authors: Wang Guanxing , Zhang Fan , Wang Li , Zeng Chen , Shi Xiaonan , Xiao Xiong
Published: June 2023

Introduction: Precipitation in the upstream region of the Heihe River basin (UHRB) in the northeastern of the Tibetan Plateau, which is the main water source of the basin, has undergone drastic changes in extreme climate events in recent decades. In addition to the amount of precipitation, the type of precipitation has a substantial impact on hydrological processes.

Methods: In this study, we compared the results from three methods aimed at improving precipitation type estimation based on daily precipitation type records for 24 discontinuous years. Based on the precipitation type distinction, we examined the spatial and temporal changes in the total precipitation, rainfall, snowfall and air temperature at the six stations as well as the spatial average of the UHRB during the past 62 years. We also analyzed changes in the quantity, duration magnitude, and frequency of extreme precipitation using the RClimDex model and statistical analysis.

Results: The probability of detection value of the T3.6_4.5 method was 1.9%, indicating the estimation was closest to actual records. The analyses based on precipitation type diving showed that rainfall accounts for an average of 81.9% of the total precipitation received in a year. In the context of large scale climate warming, temperatures at all six stations increased significantly, but precipitation changes were only apparent at about half of the stations which were located in regions of higher elevation and influenced by both ENSO and the East Asian monsoon. Analysis of the spatial averages in the UHRB revealed that the annual drought events (CDD) were significantly alleviated, and that the growing season length (GSL) was significantly extended. The annual total precipitation, rainfall, and extreme precipitation indices (P99P, P95P, R95P, and SDII) increased in magnitude, and the frequency of extreme precipitation events (P10mm) also significantly increased.

Discussion: The findings of this study indicate that under the background of climate warming, the changes of precipitation patterns in the UHRB which may have resulted in bringing better vegetation growth, but also the increasingly frequent extreme rainfall events may pose challenges to growing extreme rainfall events to agriculture and other human activities in local and downstream areas.
Variance component estimates, phenotypic characterization, and genetic evaluation of bovine congestive heart failure in commercial feeder cattle
Authors: Buchanan Justin W. , Flagel Lex E. , MacNeil Michael D. , Nilles Ashley R. , Hoff Jesse L. , Pickrell Joseph K. , Raymond Randall C.
Published: June 2023

The increasing incidence of bovine congestive heart failure (BCHF) in feedlot cattle poses a significant challenge to the beef industry from economic loss, reduced performance, and reduced animal welfare attributed to cardiac insufficiency. Changes to cardiac morphology as well as abnormal pulmonary arterial pressure (PAP) in cattle of mostly Angus ancestry have been recently characterized. However, congestive heart failure affecting cattle late in the feeding period has been an increasing problem and tools are needed for the industry to address the rate of mortality in the feedlot for multiple breeds. At harvest, a population of 32,763 commercial fed cattle were phenotyped for cardiac morphology with associated production data collected from feedlot processing to harvest at a single feedlot and packing plant in the Pacific Northwest. A sub-population of 5,001 individuals were selected for low-pass genotyping to estimate variance components and genetic correlations between heart score and the production traits observed during the feeding period. At harvest, the incidence of a heart score of 4 or 5 in this population was approximately 4.14%, indicating a significant proportion of feeder cattle are at risk of cardiac mortality before harvest. Heart scores were also significantly and positively correlated with the percentage Angus ancestry observed by genomic breed percentage analysis. The heritability of heart score measured as a binary (scores 1 and 2 = 0, scores 4 and 5 = 1) trait was 0.356 in this population, which indicates development of a selection tool to reduce the risk of congestive heart failure as an EPD (expected progeny difference) is feasible. Genetic correlations of heart score with growth traits and feed intake were moderate and positive (0.289–0.460). Genetic correlations between heart score and backfat and marbling score were −0.120 and −0.108, respectively. Significant genetic correlation to traits of high economic importance in existing selection indexes explain the increased rate of congestive heart failure observed over time. These results indicate potential to implement heart score observed at harvest as a phenotype under selection in genetic evaluation in order to reduce feedlot mortality due to cardiac insufficiency and improve overall cardiopulmonary health in feeder cattle.
Whole exome sequencing identified five novel variants in CNTN2, CARS2, ARSA, and CLCN4 leading to epilepsy in consanguineous families
Authors: Abdulkareem Angham Abdulrhman , Zaman Qaiser , Khan Hamza , Khan Sabar , Rehman Gauhar , Tariq Nabeel , Ahmad Mashal , Owais Muhammad , Najumuddin  , Muthaffar Osama Yousef , Bibi Fehmida , Khang Rin , Ryu Seung Woo , Naseer Muhammad Imran , Jelani Musharraf
Published: June 2023

Introduction: Epilepsy is a group of neurological disorders characterized by recurring seizures and fits. The Epilepsy genes can be classified into four distinct groups, based on involvement of these genes in different pathways leading to Epilepsy as a phenotype. Genetically the disease has been associated with various pathways, leading to pure epilepsy-related disorders caused by CNTN2 variations, or involving physical or systemic issues along with epilepsy caused by CARS2 and ARSA, or developed by genes that are putatively involved in epilepsy lead by CLCN4 variations.

Methods: In this study, five families of Pakistani origin (EP-01, EP-02, EP-04, EP-09, and EP-11) were included for molecular diagnosis.

Results: Clinical presentations of these patients included neurological symptoms such as delayed development, seizures, regression, myoclonic epilepsy, progressive spastic tetraparesis, vision and hearing impairment, speech problems, muscle fibrillation, tremors, and cognitive decline. Whole exome sequencing in index patients and Sanger sequencing in all available individuals in each family identified four novel homozygous variants in genes CARS2: c.655G>A p.Ala219Thr (EP-01), ARSA: c.338T>C: p.Leu113Pro (EP-02), c.938G>T p.Arg313Leu (EP-11), CNTN2: c.1699G>T p.Glu567Ter (EP-04), and one novel hemizygous variant in gene CLCN4: c.2167C>T p.Arg723Trp (EP-09).

Conclusion: To the best of our knowledge these variants were novel and had not been reported in familial epilepsy. These variants were absent in 200 ethnically matched healthy control chromosomes. Three dimensional protein analyses revealed drastic changes in the normal functions of the variant proteins. Furthermore, these variants were designated as “pathogenic” as per guidelines of American College of Medical Genetics 2015. Due to overlapping phenotypes, among the patients, clinical subtyping was not possible. However, whole exome sequencing successfully pinpointed the molecular diagnosis which could be helpful for better management of these patients. Therefore, we recommend that exome sequencing be performed as a first-line molecular diagnostic test in familial cases.
Pathogenic role of S100 proteins in psoriasis
Authors: Liang Huifang , Li Junqin , Zhang Kaiming
Published: June 2023

Psoriasis is a chronic inflammatory skin disease. The histopathological features of psoriasis include excessive proliferation of keratinocytes and infiltration of immune cells. The S100 proteins are a group of EF-hand Ca2+-binding proteins, including S100A2, -A7, -A8/A9, -A12, -A15, which expression levels are markedly upregulated in psoriatic skin. These proteins exert numerous functions such as serving as intracellular Ca2+ sensors, transduction of Ca2+ signaling, response to extracellular stimuli, energy metabolism, and regulating cell proliferation and apoptosis. Evidence shows a crucial role of S100 proteins in the development and progress of inflammatory diseases, including psoriasis. S100 proteins can possibly be used as potential therapeutic target and diagnostic biomarkers. This review focuses on the pathogenic role of S100 proteins in psoriasis.