bluuebunny/crossref_metadata_2025 huggingface dataset:
Before Helene’s Landfall: Analysis of Disaster Risk Perceptions and Preparedness Assessment in the Southeastern United States in 2023
Authors: Hong Young-Rock , Chu Haoran , Xie Zhigang , Dalisay Francis
Published: January 2025

Hurricane Helene’s catastrophic impact on the Southeastern United States highlighted the critical importance of disaster preparedness. This study analyzes data from FEMA’s 2023 National Household Survey to examine pre-Helene disaster risk perception and preparedness levels among residents of six Southeastern states: Florida, Georgia, North Carolina, South Carolina, Tennessee, and Virginia. Our aim was to assess baseline preparedness and gain insights that could inform future disaster planning. The analysis revealed significant inter-state variations in risk perceptions, with Florida residents showing the highest awareness (84% believing a disaster was likely or very likely) and Virginia residents the lowest (63%). Perceived primary threats varied geographically, with hurricanes dominating concerns in coastal states (78% in Florida) and tornadoes in inland areas (68% in Georgia). Despite these differences, concerns about losing access to essential services during disasters were consistent across all states, with over 60% of residents extremely concerned about energy and food/shelter disruptions. While self-reported confidence in disaster preparedness was high across all states, there was a notable discrepancy between this confidence and residents’ estimated ability to manage without power or water. For instance, only 47% of Florida residents believed they could manage without power for more than one week despite their high-risk perception. Home or renters’ insurance coverage ranged from 65% in Florida to 77% in South Carolina. Hazard-specific insurance varied widely, with hurricane insurance coverage at 53% in Florida compared to about 12% in Tennessee. Our findings provide timely insights into the state of disaster preparedness in the wake of Helene, emphasizing more need for tailored, region-specific approaches to disaster preparedness and risk communication. The discrepancies between perceived and actual preparedness highlighted by this study can inform more effective strategies for enhancing community resilience in the face of increasing extreme weather events driven by climate change.
Measuring Navigational Health Literacy in Russia: Validation of the HLS19-NAV-RU
Authors: Drapkina Oxana , Molosnov Artemii , Tyufilin Denis , Lopatina Maria , Medvedev Viktor , Chigrina Valeriya , Kobyakova Olga , Deev Ivan , Griese Lennert , Schaeffer Doris , Griebler Robert , Tuillet Polina , Kontsevaya Anna
Published: January 2025

Structures and regulations of healthcare systems in many countries have become increasingly complex and difficult for patients and users to navigate. Thus, more than ever before, navigational health literacy (NAV-HL) is needed by patients. There are no data on NAV-HL in Russia due to the lack of suitable concepts and measuring tools. Therefore, the study aimed to validate the HLS19 navigational health literacy assessment tool (HLS19-NAV) for the Russian-speaking population. This study provides a comprehensive overview of the validation process, including a comprehensibility check and psychometric analysis. Overall, the results of the HLS19-NAV-RU validation demonstrate the partial validity of the NAV-HL tool in the Russian language. Nevertheless, the instrument can be recommended for further research and use in health literacy studies in Russia.
Sexual Dysfunction in Patients with Chronic Non-Genital Physical Disease: An Umbrella Review
Authors: Leemans Charlotte , Van den Broucke Stephan , Jeitani Céline
Published: January 2025

Many primary studies and reviews have been published on the influence of chronic diseases (CDs) on sexual dysfunction (SD), but CDs not involving the genitals are less well represented in the literature. Aim: To determine the prevalence of SD in patients with non-genital CD and assess the overall impact on sexual functioning. Methods: An umbrella review was performed of published systematic reviews on the relationship between the occurrence of CDs not involving the genitals and patients’ sexual functioning, following the PRISMA guidelines. PubMed, EMBASE, the Cochrane Library, PsycInfo, Scopus, and CINAHL were used to find publications for inclusion in the review, with two independent researchers performing the search and selection of articles, data extraction, and quality assessment. The relative risk (RR) or odds ratio (OR) with 95% confidence was used as an indicator of the association between CD and SD across studies. The quality of studies included in the review was assessed using Amstar-2. Outcomes: Forty-three systematic reviews, thirty of which included a meta-analysis, met the inclusion criteria, representing a total of 880,586 patients (756,629 (85.9%) men, 68,388 (7.8%) women, and 55,569 (6.3%) unspecified). Results: Among men, CD is associated with erectile dysfunction, and among females, with lower levels of desire, arousal, lubrication, orgasm, and sexual satisfaction and with increased pain during intercourse. For both men and women, depression, anxiety, and fatigue are also reported, while women with CD are more affected by a poor body image than men. Clinical implications: Patients with CD, especially females, should be more routinely assessed for the impact of their condition on sexual functioning. The impact of CD on men’s sexuality has been extensively studied in terms of erectile capacity, but other aspects of their sexuality are largely neglected. Strengths and limitations: This is the first umbrella review to bring together the documented findings regarding sexual dysfunction among patients with various non-genital CDs. While the heterogeneity of the CDs makes the study unique and clinically relevant, it renders the interpretation of the results more difficult. The overrepresentation of men in existing studies reflects the current state of research but limits the applicability of the findings for women. Conclusions: Women and men with non-genital CDs can suffer from SD or reduced sexual function. Health professionals should pay more attention to managing these sexual disorders, even when the disease does not affect the genitals.
Metabolic Profiling of Breast Cancer Cell Lines: Unique and Shared Metabolites
Authors: Gallo Mariana , Ferrari Elena , Brugnoli Federica , Terrazzan Anna , Ancona Pietro , Volinia Stefano , Bertagnolo Valeria , Bergamini Carlo M. , Spisni Alberto , Pertinhez Thelma A. , Bianchi Nicoletta
Published: January 2025

Breast Cancer (BrCa) exhibits a high phenotypic heterogeneity, leading to the emergence of aggressive clones and the development of drug resistance. Considering the BrCa heterogeneity and that metabolic reprogramming is a cancer hallmark, we selected seven BrCa cell lines with diverse subtypes to provide their comprehensive metabolome characterization: five lines commonly used (SK-Br-3, T-47D, MCF-7, MDA-MB-436, and MDA-MB-231), and two patient-derived xenografts (Hbcx39 and Hbcx9). We characterized their endometabolomes using 1H-NMR spectroscopy. We found distinct metabolite profiles, with certain metabolites being common but differentially accumulated across the selected BrCa cell lines. High levels of glycine, lactate, glutamate, and formate, metabolites known to promote invasion and metastasis, were detected in all BrCa cells. In our experiment setting were identified unique metabolites to specific cell lines: xanthine and 2-oxoglutarate in SK-Br-3, 2-oxobutyrate in T-47D, cystathionine and glucose-1-phosphate in MCF-7, NAD+ in MDA-MB-436, isocitrate in MDA-MB-231, and NADP+ in Hbcx9. The unique and enriched metabolites enabled us to identify the metabolic pathways modulated in a cell-line-specific manner, which may represent potential candidate targets for therapeutic intervention. We believe this study may contribute to the functional characterization of BrCa cells and assist in selecting appropriate cell lines for drug-response studies.
Biodistribution of Polyaldehydedextran Nanoparticle-Encapsulated Epirubicin in Ovarian Tumor-Bearing Mice via Optical Imaging
Authors: Kośnik Wioletta , Sikorska Hanna , Kiciak Adam , Ciach Tomasz
Published: January 2025

This study investigates the biodistribution of polysaccharide-based nanoparticles loaded with epirubicin (POLEPI) compared to epirubicin hydrochloride (EPI) in naïve female nude mice following a single intravenous dose. The inherent fluorescence of epirubicin was tracked using Newton 7 animal imager and Varioskan. Initial whole-animal optical imaging failed to reliably detect epirubicin distribution, necessitating ex vivo imaging of key tissues harvested at intervals between 10 min and 48 h post-injection. Optimal imaging conditions were established using a 5 s exposure time with excitation (Ex)/emission (Em) at 480 nm/550 nm. The biodistribution of POLEPI was further evaluated in both naïve mice and immunocompromised mice bearing patient-derived ovarian tumors. Unlike epirubicin, POLEPI exhibited notable tissue distribution within 3 h post-injection. By 48 h, fluorescence signals were undetectable in both models, although non-tumored animals exhibited persistent signals. In both models, the liver was the primary organ for POLEPI accumulation, with lower levels in tumored mice. Interestingly, brain fluorescence was higher in POLEPI-treated mice compared to those receiving epirubicin. Neither POLEPI nor epirubicin accumulated in the spleen or bone marrow. In tumors, POLEPI fluorescence peaked at 24 h, with levels 2.1 times higher than in the epirubicin-treated group over a 48 h period. Furthermore, POLEPI uptake in tumors exceeded that in healthy ovaries, with the most significant tumor-to-healthy-ovary ratio observed between 6 and 24 h post-injection. These findings demonstrate that POLEPI, a novel polyaldehydedextran nanoparticle formulation, exhibits enhanced accumulation and retention in tumor tissue compared to epirubicin, with preferential distribution to the orthotopic tumor-bearing ovary over healthy ovarian tissue. The inherent fluorescence of epirubicin provided a rapid and cost-effective means of estimating biodistribution, although the limitations of this method—particularly, the inability to differentiate between the parent drug and its metabolites—were acknowledged.
Expression Patterns of Escape Genes in Turner Syndrome Fibroblasts and Induced Pluripotent Stem Cells
Authors: Byun Seki , Yoon Sang-Hoon , Hong Yean-Ju , Jang Hyun-Sik , Seo Bong-Jong , Choi Gyu-Tae , La Hyeonwoo , Lee Je-Woo , Hong Kwonho , Do Jeong-Tae
Published: January 2025

Turner syndrome (TS) is an X monosomy-related disorder caused by X chromosome nondisjunction during embryonic development. Patients with TS have only one intact X chromosome, with the other either completely or partially lost. TS affects various tissues, including the liver, kidneys, brain, cardiovascular system, and ovaries. These abnormalities are suggested to involve an altered dosage of escape genes that evade X chromosome inactivation. However, the mechanisms and roles of these escape genes in the TS phenotype remain unclear. We hypothesized that the expression levels of escape genes differ between wild-type (WT) and TS cell lines. In this study, we generated induced pluripotent stem cell (iPSC) lines from WT and TS fibroblasts and examined the expression levels of escape genes in both undifferentiated fibroblasts and reprogrammed iPSCs from WT and TS samples. The reprogrammed WT and TS iPSCs exhibited general characteristics of pluripotency, including the expression of pluripotency markers and the potential to differentiate into all three germ layers. Forty-five escape genes were differentially expressed between the WT and TS cell lines. Among these, five genes (ATP7A, PHKA1, EBP, ZFX, and SMC1A) were suggested to be implicated in the TS phenotype. However, further studies using additional cell lines are necessary to clarify the correlation between TS and escape genes.
Short-Term Fasting Induces Hepatocytes’ Stress Response and Increases Their Resilience
Authors: Prša Patrik , Miller Izak Patrik , Kramar Barbara , Šuput Dušan , Milisav Irina
Published: January 2025

Fasting leads to a range of metabolic adaptations that have developed through evolution, as humans and other mammals have unequal access to food over the circadian cycle and are therefore adapted to fasting and feeding cycles. We have investigated the role of a single fasting episode in rats in triggering the stress response of liver hepatocytes. Since the stress responses were observed in both animals and isolated cells, we investigated whether the effects of the animal stressor could persist in the cells after isolation. By measuring staurosporine-induced apoptosis, stress signalling, and oxidative and antioxidant responses in hepatocytes from fasted and ad libitum-fed animals, we found that only fasting animals elicited a stress response that prevented caspase-9 activation and persisted in isolated cells. The addition of glucose oxidase, a hydrogen peroxide-producing enzyme, to the cells from ad libitum-fed animals also led to a stress response phenotype and prevented the activation of caspase-9. A single fasting episode thus leads to a stress response in normal hepatocytes, with hydrogen peroxide as a second messenger that reduces the initiation of apoptosis. This finding is the first characterisation of a mechanism underlying the effects of fasting and provides a basis for the development of methods to increase the resilience of cells. These findings need to be taken into account when interpreting the results obtained in animal and cell research models to account for the effects of overnight fasting used in many laboratory protocols. The research results also form the basis for the development of clinical applications to increase the resistance of transplants and to improve the fitness of hepatocytes under acute stress conditions in liver and some metabolic diseases.
Characterization of Metabolic Patterns in Mouse Spermatogenesis and Its Clinical Implications in Humans
Authors: Wang Jiachen , Chen Mengqi , Yao Ying , Zhu Mengyuan , Jiang Yingtong , Duan Jiawei , Yuan Yan , Li Laihua , Chen Minjian , Sha Jiahao
Published: January 2025

Spermatogenesis is a complex process requiring precisely controlled metabolic adaptations. Although the genetic and cellular aspects of spermatogenesis have been extensively studied, the underlying metabolic mechanisms remain largely underexplored. In this study, we utilized STA-PUT technology to separate three key cell types involved in mouse spermatogenesis: pachytene spermatocytes (PAC), round spermatids (RS), and elongated spermatids (ES). A comprehensive untargeted metabolomic analysis revealed significant metabolic changes during spermatogenesis, such as reduced methylation-related metabolites and increased glycolytic intermediates and TCA cycle metabolites during ES. Moreover, metabolic differences between germ cells and somatic cells (Leydig and Sertoli cells) were highlighted, particularly in steroidogenesis and lipid metabolism. To investigate clinical relevance, we analyzed human seminal plasma. Samples from individuals with azoospermia displayed significant metabolic abnormalities, including reduced methionine, tryptophan, and arginine, which play vital roles in sperm development. Pathway enrichment analysis revealed disturbances in the metabolism of nucleotide, amino acid, and energy in azoospermia, suggesting potential biomarkers of male infertility. Our findings provide a comprehensive metabolic profile of spermatogenesis and suggest that metabolic alterations may be significant contributors to male infertility, particularly in cases of azoospermia.
Molecular Docking Study and 3D-QSAR Model for Trans-Stilbene Derivatives as Ligands of CYP1B1
Authors: Dutkiewicz Zbigniew , Mikstacka Renata
Published: January 2025

Scientific research on stilbenes is conducted for their chemopreventive and therapeutic properties. In experimental studies, natural and synthetic trans-stilbenes exhibit antioxidant, anti-inflammatory, cardioprotective, and anticancer effects. The antitumor activity of some natural and synthetic stilbenes is associated with their interaction with cytochrome P450 family 1, which leads to the inhibition of procarcinogen activation. In the present study, three-dimensional quantitative structure–activity relationship analysis (3D-QSAR) was performed on a series of forty-one trans-stilbene derivatives to identify the most significant features of the molecules responsible for their CYP1B1 inhibitory activity. The developed 3D-QSAR model presented a cross-validated correlation coefficient Q2 of 0.554. The model’s predictive ability was confirmed by external validation (r2 = 0.808). The information provided by 3D-QSAR analysis is expected to be valuable for the rational design of novel CYP1B1 inhibitors.
Alzheimer’s Disease: Exploring Pathophysiological Hypotheses and the Role of Machine Learning in Drug Discovery
Authors: Dominguez-Gortaire Jose , Ruiz Alejandra , Porto-Pazos Ana Belen , Rodriguez-Yanez Santiago , Cedron Francisco
Published: January 2025

Alzheimer’s disease (AD) is a major neurodegenerative dementia, with its complex pathophysiology challenging current treatments. Recent advancements have shifted the focus from the traditionally dominant amyloid hypothesis toward a multifactorial understanding of the disease. Emerging evidence suggests that while amyloid-beta (Aβ) accumulation is central to AD, it may not be the primary driver but rather part of a broader pathogenic process. Novel hypotheses have been proposed, including the role of tau protein abnormalities, mitochondrial dysfunction, and chronic neuroinflammation. Additionally, the gut–brain axis and epigenetic modifications have gained attention as potential contributors to AD progression. The limitations of existing therapies underscore the need for innovative strategies. This study explores the integration of machine learning (ML) in drug discovery to accelerate the identification of novel targets and drug candidates. ML offers the ability to navigate AD’s complexity, enabling rapid analysis of extensive datasets and optimizing clinical trial design. The synergy between these themes presents a promising future for more effective AD treatments.
LRP4 and Agrin Are Modulated by Cartilage Degeneration and Involved in β-Catenin Signaling in Human Articular Chondrocytes
Authors: Naniwa Shuichi , Nishida Keiichiro , Yoshida Aki , Nasu Yoshihisa , Nakahara Ryuichi , Ohtsuki Takashi , Hotta Yoshifumi , Shimizu Noriyuki , Ichikawa Chinatsu , Lin Deting , Otsuka Noriaki , Ozaki Toshifumi
Published: January 2025

We investigated the roles of low-density lipoprotein receptor-related protein (LRP) 4 and its ligand Agrin in the pathophysiology of cartilage degeneration. Immunohistochemical analysis of human normal articular cartilage and cartilage tissues from patients with osteoarthritis (OA) obtained during surgery of the knee joint showed marked LRP4 expression in the early stages of OA, which then decreased with cartilage degeneration, whereas Agrin was consistently increased with cartilage degeneration. In normal human articular chondrocytes (NHACs), mild cyclic tensile strain (CTS) (0.5 Hz, 5% elongation, 2 h) increased the expression of LRP4 and aggrecan (ACAN), while intense CTS (0.5 Hz, 10% elongation, 6 h) increased the expression of Agrin without affecting LRP4 expression. Treatment with recombinant human (rh) Agrin downregulated the mRNA expression of LRP4 and ACAN, but upregulated the expression of LRP5/6, SRY-box transcription factor 9 (SOX9), Runt-related transcription factor 2 (RUNX2), and a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4). Immunocytochemistry and Western blot analysis showed that rhAgrin treatment upregulated the expression of β-catenin and SOX9. Agrin knockdown by siAGRN transfection partially reduced the nuclear protein expression of β-catenin, which was increased with intense CTS. LRP4 knockdown by siLRP4 transfection increased the expression of LRP5/6, SOX9, RUNX2, ADAMTS-4, and Agrin. These results suggested that intense CTS increases the expression of Agrin, which might interfere with the role of LRP4 in the inhibition of LRP5/6 and their downstream β-catenin signaling, leading to cartilage degeneration.
Fibrinogen Alpha Chain as a Potential Serum Biomarker for Predicting Response to Cisplatin and Gemcitabine Doublet Chemotherapy in Lung Adenocarcinoma: Integrative Transcriptome and Proteome Analyses
Authors: Raungrut Pritsana , Jirapongsak Jirapon , Tanyapattrapong Suchanan , Bunsong Thitaya , Ruklert Thidarat , Kueakool Kannika , Thongsuksai Paramee , Nakwan Narongwit
Published: January 2025

Cisplatin combined with gemcitabine, a doublet regimen, is the first-line treatment for patients with advanced lung adenocarcinoma (ADC); however, the treatment response remains poor. This study aimed to identify potential biomarkers for predicting response to cisplatin and gemcitabine. Tissue transcriptome and blood proteome analyses were conducted on 27 patients with lung ADC. Blood-derived proteins that reflected tissue-specific biomarkers were obtained using Venn diagrams. The candidate proteins were validated by Western blotting. Lentivirus-mediated short hairpin RNA interference was used to verify the functional roles of the candidate proteins in human A549 cells. We identified 417 differentially expressed genes, including 52 upregulated and 365 downregulated genes, and 31 differentially expressed proteins, including 26 upregulated and 5 downregulated proteins. Integrative analysis revealed the presence of alpha-1-acid glycoprotein 1 (A1AG1) and fibrinogen alpha chain (FGA or FIBA) in both the tissue and serum. FGA levels were elevated in responders compared to non-responders, and reduced serum FGA levels were correlated with resistance to this regimen. Moreover, FGA knockdown in A549 cells resulted in resistance to the doublet regimen. Our findings indicate that FGA is a tissue-specific serum protein that may function as a blood-based biomarker to predict the response of patients with lung ADC to cisplatin plus gemcitabine chemotherapy.
Genome-Wide Analysis, Identification, and Transcriptional Profile of the Response to Abiotic Stress of the Purple Acid Phosphatases (PAP) Gene Family in Apple
Authors: Liu Hong-Chao , Rao Lei , Meng Jia-Hui , Zuo Wen-Teng , Sun Ting-Ting
Published: January 2025

Purple acid phosphatases (PAPs) play a significant role in plant phosphorus nutrition and can not only release phosphorus from the soil but also regulate the distribution of phosphorus in plants throughout their entire growth and development process. Moreover, members of the PAP protein family exert a more extensive influence on plant mineral homeostasis, developmental processes, and stress responses. Three clusters of purple acid phosphatases, including 31 putative genes, were identified in apples (Malus domestica) by searching the Genome Database for Rosaceae. The structure, chromosomal distribution and location, phylogeny, motifs, and cis-acting elements in the gene promoter regions of the MdPAP gene family are reviewed. These genes exhibit different expression patterns in different tissues. For example, almost all MdPAP genes are strongly expressed in the roots, except for MdPAP10, MdPAP12, and MdPAP27. Similarily, all MdPAPs were expressed in the leaves while the transcript levels of MdPAP7, MdPAP10, MdPAP15, MdPAP21, MdPAP24, MdPAP26, MdPAP29, and MdPAP30 were highest in apple flowers. Overall, the expression of the 31 genes significantly changed in either the roots or leaves following the application of phosphorus and/or drought stress. These results indicate that MdPAP family members play a role in plant adaptation to adverse environments. This work explores the adaptative responses to phosphorus and/or drought conditions in apple and establishes a foundation for an enhanced comprehension of the evolution of PAP families and the exploration of the genes of interest.
Enhancing Foreign Speakers’ Satisfaction in Learning Indonesian Language with a Gamified Multiplatform Approach
Authors: Irfan Rifqi Imaduddin , Arifin Yulyani
Published: January 2025

This study examines how gamification can improve the satisfaction level of foreign learners studying the Indonesian language using the innovative multiplatform application named Belajar Indo. In consideration of the Indonesian government’s heightened focus on language proficiency for foreign workers and students, this study examines the challenges encountered in Bahasa Indonesia for Foreign Speakers (BIPA) programs. Integrating gamified elements into a Progressive Web App (PWA) presents an engaging alternative to traditional learning resources, which frequently fall short in terms of interactivity and accessibility. Data gathered from surveys, online observation, and user engagement metrics indicate that gamification greatly improves learner motivation, resulting in a success rate of 93.51% and a Net Promoter Score (NPS) of 8.11. The findings demonstrate that gamification enhances language acquisition while simultaneously cultivating a greater enthusiasm for learning Indonesian, proving advantageous for both beginners and advanced learners. This study advances the domain of educational technology by presenting a model that integrates language acquisition with digital innovation, highlighting gamified learning as an effective instrument for foreign language education. Future recommendations involve fine-tuning user interface components to improve usability and maintain elevated engagement levels.
Eradication of Yellow Crazy Ants, Anoplolepis gracileps Smith, from Lismore and Statistical Proof of Freedom Using Scenario Tree Analysis
Authors: Henderson Robyn , Charlton Scott , Fraser Catherine , Moloney Barbara , Sergeant Evan S. G. , Dominiak Bernard C.
Published: January 2025

Yellow crazy ants (YCAs) are an invasive ant with a pantropical distribution, largely due to the international movements of ships and produce. This invasive ant has the capacity to impact a broad range of environmental, domestic and agricultural situations and has the ability to develop into supercolonies and dominate landscapes if uncontrolled. YCAs have been detected in several locations in Australia. During 2018 in New South Wales, YCAs were detected in two locations in the Lismore region. Several awareness techniques were used to gain community support and engagement in the response program. The eradication program relied on the insecticide fipronil (several formulations), and the program subsequently used surveillance data to demonstrate that eradication had been achieved. We used the scenario tree analysis with stochastic models to estimate the likelihood of eradication. We combined the results of the passive and active surveillance systems to predict a 70.4% (62.7–80.7) probability of freedom of detecting one nest, 84.4% (73.9–94.4) probability of freedom for two nests and 98% (93.1–99.9) probability of freedom for five nests. The results from the scenario tree analysis were used to inform program managers regarding the termination of the eradication and surveillance activities.
Aortic Remodeling After False Lumen Embolization in Aortic Dissection
Authors: Halloum Nancy , Meyer Anna-Sophie , Wenkel Martin , Dohle Daniel-Sebastian , Youssef Marwan , Dorweiler Bernhard , Treede Hendrik , El Beyrouti Hazem
Published: January 2025

Background/Objectives: Retrograde false lumen perfusion after thoracic endovascular aortic repair (TEVAR) can compromise positive remodeling and clinical outcomes. The aim of this study is to describe the feasibility and outcomes of a false lumen thrombosis technique. Methods: A single-center, retrospective analysis of patients between January 2017 and January 2022 who underwent TEVAR in conjunction with false lumen embolization. Results: Twelve patients (83% type A, 17% type B; 33% with frank rupture; mean age 65 years; eleven men) underwent 13 false lumen embolization procedures with a 92% technical success rate and a 100% clinical success rate. Positive aortic remodeling was observed in all the patients. The maximum thoracic aortic diameter remained stable (p = 0.526) but the true lumen increased from 22 to 33 mm (p = 0.009) and the false lumen decreased from 41 to 20 mm (p = 0.076) after a median follow-up of 31 months. Conclusions: False lumen embolization using the candy-plug is safe and promotes positive aortic remodeling.
Risk of Postoperative Infection in Total Knee Arthroplasty Patients with Preoperative Methicillin-Resistant Staphylococcus aureus (MRSA) Colonization
Authors: Areti Aruni , Ratcliff Terrul , Mittal Mehul M. , Wukich Dane K. , Chen Antonia F. , Sambandam Senthil N.
Published: January 2025

Background/Objectives: While methicillin-resistant Staphylococcus aureus (MRSA) colonization is a known risk factor for surgical site infections, no definitive recommendations exist regarding preoperative S. aureus screening and decolonization protocols due to inconclusive evidence in Orthopedic Surgery. This study aimed to examine the correlation between preoperative MRSA colonization and postoperative infections in Total Knee Arthroplasty (TKA) patients. Methods: Data from 2005 to 2023 were collected from TriNetX, a global health research network, reviewing 237,360 unique patients. TKA patients were assigned under Current Procedural Terminology, while International Statistical Classification of Diseases Codes were used to identify preoperative comorbidities and postoperative complications. Demographic and analytical statistics were compared between MRSA-positive and control groups before and after propensity matching. Results: The MRSA-positive group had a significantly (p < 0.001) higher proportion of patients over 65 years (47.17% vs. 38.46%), obesity (41.76% vs. 34.67%), smoking disorders (33.36% vs. 19.73%), and diabetes (25.06% vs. 19.85%) compared to the control group. Postoperative complications were significantly (p < 0.001) more frequent in the MRSA-positive group, specifically periprosthetic joint infection (PJI) (4.11% vs. 0.79%, OR = 5.40), deep (0.12% vs. 0.01%, OR = 11.15) and superficial (0.37% vs. 0.09%, OR = 4.17) surgical site infections, and wound dehiscence (1.11% vs. 0.52%, OR = 2.13). The matched analysis confirmed significantly (p < 0.001) higher rates of PJI (4.39% vs. 1.18%, OR = 3.59). Conclusions: Our results illustrated preoperative colonization of MRSA as associated with an increased risk of wound-related complications. Surgeons and patients must consider preoperative MRSA colonization status when deciding if TKA is an optimal treatment option.
Analysis of Natural Clinical Crown Height Changes in Central Incisors and First Molars from Age 8 to 18: A Retrospective Digital Study
Authors: Huanca Ghislanzoni Luis , Boesiger Jean , Mourgues Thomas , González-Olmo María José , Romero Martin
Published: January 2025

Objective: This thesis aims to digitally study the natural longitudinal evolution of clinical crown height in maxillary and mandibular central incisors and first molars between 8 and 18 years of age. Methods: A total of 31 subjects (17 females and 14 males) were selected for the study. Their plaster study models were converted to digital format using a three-dimensional scanner. Digital anatomical landmarks were placed on the central incisors and first molars of all dental arches. The clinical crown height of the central incisors and first molars was then analyzed digitally and longitudinally, based on the position of the marginal gingiva on the vestibular surface of the teeth in question. Results: The clinical crown height of maxillary and mandibular central incisors and first molars increased significantly with age (p-value < 0.001) from 8 to 18 years. On average, the clinical crown height increased by 1.5 mm for the upper central incisors and 0.8 mm for the mandibular central incisors. The clinical crown height of the upper first molar increased on average by 2.7 mm and 2.1 mm for mandibular first molars. Conclusions: The clinical crown height of maxillary and mandibular central incisors and first molars appears to continue increasing beyond 18 years of age. These results suggest that the position of the marginal gingiva on the buccal surface of the teeth is not stable at 18 years of age.
The Effect of Osteopathic Visceral Manipulation on Quality of Life and Postural Stability in Women with Endometriosis and Women with Pelvic Organ Prolapse: A Non-Controlled Before–After Clinical Study
Authors: Wójcik Małgorzata , Kampioni Małgorzata , Hudáková Zuzana , Siatkowski Idzi , Kędzia Witold , Jarząbek-Bielecka Grażyna
Published: January 2025

Background: Visceral manipulation is a soft tissue manual work technique that originates from and is classified within the Osteopathic Manipulative Technique (OMT), focusing on the fascial tissue mobilisation of the visceral system. Manual therapy on internal organs is based on anatomy, physiology and physics. Methods: Sixty women with endometriosis and pelvic organ prolapse, aged 41.5 ± 12.02, participated in the study. The women had manipulation therapy once a week for 5 weeks. The World Health Organisation Quality of Life BREF questionnaire and a postural stability assessment were administered before and after performing visceral manipulation. Results: The p-value = 0.0093 obtained in the group with prolapses and the p-value = 0.0001 in the group with endometriosis indicated that the applied visceral manipulation improved the women’s quality of life. No effect of therapy was observed on postural stability. Conclusions: Visceral manipulation improved the quality of life of both study groups of women. A significant difference was also observed when comparing the two groups for area.
Pulmonary Rehabilitation in Patients with Operable Non-Small Cell Lung Cancer
Authors: Zhong Jeffrey , Trinh Ilene , Raju Shine , Hsu Melinda
Published: January 2025

Lung cancer is the leading cause of cancer-related death worldwide, and patients with operable early-stage NSCLC are typically managed surgically. While effective, surgical resection can significantly impact pulmonary function and quality of life. Pulmonary rehabilitation (PR) is a comprehensive, multimodal approach that is an established cornerstone in the treatment of COPD. It has similarly demonstrated multiple benefits in patients with lung cancer who have undergone lobectomy or resection by improving pulmonary function, increasing exercise tolerance, improving nutritional status, providing psychological support, and enhancing quality of life. Despite this, PR for early-stage operable NSCLC is oftentimes not standardized, and challenges to adherence remain. In this review, we examine the components of PR, the role of PR in pre- and postoperative settings in patients with early-stage NSCLC, implementation strategies for PR, and future directions and challenges of PR in operable NSCLC.
Keeping It Simple—Pain Science Education for Patients with Chronic Pain Referred to Community-Based Rehabilitation: Translation, Adaptation, and Clinical Feasibility Testing of PNE4Adults
Authors: Eiger Bettina , Rathleff Michael Skovdal , Ickmans Kelly , Rheel Emma , Straszek Christian Lund
Published: January 2025

Background/Objectives: Pain science education accommodating low health literacy is needed for people with chronic pain. The purpose of this study was to translate PNE4Kids, contextually adapt it into PNE4Adults, and test the feasibility of the newly developed pain science education program (PNE4Adults) for adult patients with chronic musculoskeletal pain in the municipality. Methods: A three-step approach was used to (1) translate PNE4Kids into Danish, (2) adapt to age and context (PNE4Adults), and (3) test the feasibility. (1) Translation was performed by a native Dane fluent in Dutch. (2) Two think-aloud group sessions were held, with therapists and end users. (3) Feasibility was tested amongst twenty adult patients with chronic musculoskeletal pain consecutively referred for rehabilitation in the municipality. The a priori success criteria were determined to be 70% acceptability and 70% understandability. Prior to inclusion of the first patient, the study was pre-registered on clinicaltrial.gov [NCT05140031]. Results: Translation was successfully performed. Both the therapist and end users found the program easy to grasp, the simplicity and interactive nature of the program ingenious, and the program to be well suited to an adult population. All patients (100%), across health literacy levels, found PNE4Adults comprehensible and acceptable. Conclusions: The aims were successfully met. Progression to a full trial is warranted and is underway.
Transcatheter Aortic Valve Implantation (TAVI) in Bicuspid Anatomy
Authors: Nikas Dimitrios N. , Lakkas Lampros , Naka Katerina K. , Michalis Lampros K.
Published: January 2025

Bicuspid aortic valve (BAV) stenosis, a common congenital condition, presents unique challenges for transcatheter aortic valve replacement (TAVI) due to anatomical variations like cusp morphology, coexisting aortopathy and calcification. TAVI offers a viable option for BAV patients with refinements in technique and technology, though ongoing research is essential to optimize patient-specific approaches and long-term results. Key considerations for TAVI in BAV include precise valve sizing, positioning, and the need for rigorous pre-procedural imaging to mitigate risks such as paravalvular leak and stroke. Early results show TAVI’s safety and efficacy are comparable to surgery, though BAV patients undergoing TAVI often are exposed to higher rates of post-procedural pacemaker implantation. Emerging data on next-generation self-expandable (SE) and balloon-expandable (BE) valves reveal that while both offer success in this complex anatomical aortic valve variation, gaps remain in the long-term durability and management of BAV-related aortopathy. This review examines the latest advancements in TAVI for BAV, emphasizing how specialized approaches and device selection address BAV’s complexities.
Hierarchical Free Vibration Analysis of Variable-Angle Tow Shells Using Unified Formulation
Authors: Iannotta Domenico Andrea , Giunta Gaetano , Kirkayak Levent , Montemurro Marco
Published: January 2025

This paper investigates the dynamic behavior of shell structures presenting variable-angle tow laminations. The choice of placing fibers along curvilinear patterns allows for a broader structural design space, which is advantageous in several engineering contexts, provided that more complex numerical analyses are managed. In this regard, Carrera’s unified formulation has been widely used for studying variable-angle tow plates and shells. This article aims to expand this formulation through the derivation of the complete formulation for a generic shell reference surface. The principle of virtual displacements is used as a variational statement for obtaining, in a weak sense, the stiffness and mass matrices within the finite element solution method. The free vibration problem of singly and doubly curved variable-angle tow shells is then addressed. The proposed approach is compared to Abaqus three-dimensional reference solutions and classical theories to investigate the effectiveness of the developed models in predicting the vibrational frequencies and modes. The results demonstrate a good agreement between the proposed approach and reference solutions.
Edge-Based Dynamic Spatiotemporal Data Fusion on Smart Buoys for Intelligent Surveillance of Inland Waterways
Authors: Zhang Ruolan , Zhao Chenhui , Liang Yu , Hu Jingfeng , Pan Mingyang
Published: January 2025

Increasing vessel traffic in narrow, winding inland waterways has heightened the risk of accidents, driving the need for improved surveillance and management. This study addresses the challenge of real-time processing and synchronization of voluminous video and AIS data for effective waterway management. We developed a surveillance method utilizing smart buoys equipped with sensors and edge computing devices, enabling dynamic spatiotemporal data fusion. The integration of AIS data with advanced computer vision techniques for target detection allows for real-time traffic analysis and provides detailed navigational dynamics of vessels. The method employs an enhanced Long Short-Term Memory network for precise trajectory prediction of AIS data and a single-stage target detection model for video data analysis. Experimental results demonstrate significant improvements in ship detection accuracy and tracking precision, with an average position prediction error of approximately 1.5 m, which outperforms existing methods. Additionally, a novel regional division and a Kalman filter-based method for AIS and video data fusion were proposed, effectively resolving the issues of data sparsity and coordinate transformation robustness under complex waterway conditions. This approach substantially advances the precision and efficiency of waterway monitoring systems, providing a robust theoretical and practical framework for the intelligent supervision of inland waterways.
Dynamic Target Hunting Under Autonomous Underwater Vehicle (AUV) Motion Planning Based on Improved Dynamic Window Approach (DWA)
Authors: Li Juan , Lu Houtong , Zhang Honghan , Zhang Zihao
Published: January 2025

A dynamic distributed target hunting method is proposed for the problem of distributed moving target hunting by multiple Autonomous Underwater Vehicles (AUVs). By integrating the improved Dynamic Window Approach (DWA) with the Rapidly-exploring Random Tree (RRT) algorithm and incorporating collision avoidance rules between AUVs into the evaluation system of the DWA, the collision avoidance rules are quantified, and corresponding evaluation functions are established. This allows for the selection of motion trajectories that comply with the collision avoidance rules from the predicted trajectory set, improving the obstacle avoidance capability during AUV motion planning and enhancing the reliability of the target hunting task. The introduction of a consistency algorithm maintains the consistency of the group task information and ensures that the hunting strategy can be adjusted promptly in the event of an AUV failure, allowing the target hunting task to continue. Polynomial regression algorithms are used to predict the moving target’s trajectory. Based on a polygonal hunting formation, the hunting potential points are dynamically allocated, and, finally, each AUV executes distributed motion planning towards the hunting potential points to form the hunting formation. Simulation results show that the proposed method achieves efficient multi-AUV-distributed dynamic target hunting.
A Mixed Integer Linear Programming Model for Rapid Rescheduling in Ship and Offshore Unit Design Projects
Authors: Kim Kyeongho , Choi Minjoo , Seo Haram , Lee Jaekyeong , Kim Jihong , Kim Shinhyo
Published: January 2025

Shipbuilding and offshore projects frequently require schedule adjustments due to unforeseen factors such as material supply delays, technical issues, and adverse weather conditions. These adjustments are often managed manually, resulting in significant time consumption and an increased risk of human error. Unlike production scheduling, little attention has been given to design scheduling, particularly in the context of rescheduling. To address this gap, this paper presents an optimization model that automates the rescheduling process for shipbuilding and offshore unit design projects. The model generates updated schedules that accommodate necessary changes while minimizing deviations from the initial schedule. In a real-world case involving 857 tasks, the model generated a schedule in under one second, preserving approximately 80% of the original schedule and achieving a 20% improvement in adherence compared to the original scheduling method. Furthermore, the model demonstrated exceptional scalability by efficiently generating optimized schedules for 108,700 tasks in under three minutes. These results demonstrate the model’s capability to provide rapid, efficient, and scalable rescheduling solutions, enabling quick and iterative refinement processes.
Antifungal Testing of Vaginal Candida Isolates in Pregnant Women: A Retrospective, Single-Center Study in Adana, Türkiye
Authors: Sucu Mete , Ünal Nevzat , Karakoyun Ayşe Sultan , Şahin İrem , Bingöl Oğuzhan , Hüner Fatih , İşlek Uzay Fatma , Ünal İlker , Metin Dilek Yeşim , Ilkit Macit
Published: January 2025

Clinical and mycological data are essential for the optimal management of patients with Candida vaginitis (CV), particularly in cases of (i) azole-resistant C. albicans vaginitis, (ii) recurrent CV, and (iii) CV in pregnant women. The present retrospective single-center study investigated the antifungal activity of six commonly used antifungals against randomly selected vaginal isolates recovered from 68 pregnant women in Adana, Türkiye, including C. albicans, petite C. glabrata, non-petite C. glabrata, and C. krusei, using the disk diffusion method at pH 4 and 7. Furthermore, the antifungal activities of fluconazole and itraconazole were also assessed using the broth microdilution method. For all isolates, the mean inhibition zone diameters were narrower for itraconazole and ketoconazole and larger for miconazole at pH 4 than pH 7 (p < 0.05). For nystatin, zone diameters were wider in C. albicans and petite C. glabrata at pH 4 (p < 0.001 and p < 0.001). Remarkably, clotrimazole was more active at pH 4 than at pH 7, except against non-petite C. glabrata isolates. Based on the broth microdilution results, the resistance rate was higher at pH 4 than at pH 7 in all isolates. Candida glabrata petite isolates exhibited MIC values 2 to 5 times higher than those of the non-petite isolates for both fluconazole and itraconazole. This study highlights the potent activity of topical antifungals (miconazole, nystatin, and clotrimazole) for the treatment of CV in pregnant women and highlights the need to identify petite and non-petite mutants of vaginal C. glabrata isolates to obtain more reliable data and for antifungal susceptibility testing prior to decision-making. The results of the two antifungal susceptibility methods were compared for C. albicans and C. glabrata isolates, and the reliability of the disk diffusion test was discussed.