bluuebunny/crossref_metadata_2025 huggingface dataset:
Building Cultures of Participation: Involving Young People in Contact with the Criminal Justice System in the Development of Drug Interventions in the United Kingdom, Denmark, Italy and Poland
Authors: Duke Karen , Gleeson Helen , Dabrowska Katarzyna , Dich Herold Maria , Rolando Sara , Thom Betsy
Published: February 2022

This article explores the participation of young people in contact with criminal justice systems in the development of drugs interventions. Interviews were undertaken with 160 young people (aged 15–25) and 66 practitioners involved in the design, delivery and commissioning of drug interventions. We analyse the key challenges in involving young people in the development of interventions including structural, organisational and individual factors. We argue that these barriers can be overcome by fostering flexible models of participation and identifying the most meaningful and appropriate approaches for involving young people at different stages and in different initiatives which consider socio-cultural contexts.
Clinical Characteristics, Surgical Management, and Prognostic Factors of Medullary Thyroid Carcinoma: A Retrospective, Single-Center Study
Authors: Wu Xin , Li Binglu , Zheng Chaoji
Published: February 2022

Purpose: Medullary thyroid carcinoma (MTC) is a rare neuroendocrine malignancy with relatively early lymphatic metastatic spread. The clinical features of MTC remain controversial owing to the low incidence rate. This study aimed to analyze the clinical characteristics, prognostic factors, and long-term follow-up of MTC. Methods: Medical records of MTC patients treated at our hospital between December 2000 and November 2020 were reviewed retrospectively. Clinicopathologic features of MTC were analyzed using univariate and multivariate analyses. Cumulative survival rates were estimated using the Kaplan-Meier method. Results: In total, 152 patients with MTC were included. The rates of central and lateral lymph node metastases (LNM) were 52.0% and 42.8%, respectively. All patients were followed up with a median follow-up time of 43.5 (17.0−76.3) months. Univariate and multivariate analyses identified two independent factors associated with progressive disease. They were lateral LNM (p < 0.001) and lymph node ratio (LNR) >1/3 (p = 0.009). The 5-, 10-, and 15-year cumulative overall survival (OS) rates of MTC were 88.2%, 83.1%, and 76.2%, respectively. The 5-, 10-, and 15-year cumulative disease-free survival (DFS) rates were 61.8%, 48.6%, and 38.2%, respectively. Patients with stage I, II, and III disease had significantly longer OS and DFS than those with stage IV disease (p < 0.001). Conclusion: MTC is a rare endocrine malignancy and LNM is common. Patients with lateral LNM and LNR >1/3 are more likely to develop progressive disease. The long-term OS rates of MTC are good, but long-term DFS rates are poor.
Wound Healing Effects of Chitosan Nanosheets/Honey Compounds in Male BALB/c Mice
Authors: Askari Masoumeh , Afshar Mohammad , Khorashadizadeh Mohsen , Zardast Mahmoud , Naghizadeh Ali
Published: February 2022

Background and aim: Up to now, proper wound care management has remained as an important clinical challenge. Chitosan nanosheets (CNSs) showed a great potential in tissue engineering, but our knowledge about their wound healing effectiveness is based on very limited data. Thus, the aim of this research was to evaluate the wound healing potential of CNSs and honey as a vehicle for these nanoparticles. Methods: The skin excisional wound injury model was made in adult male BALB/c mice ( n = 60) by creating two identical sized wounds (5mm) on either side of their dorsal midline. The animals were divided into five groups ( n = 12 each) as untreated control, honey, polyethylene glycol, and CNSs dissolved either in honey or polyethylene glycol. Animals were received their relative topical treatments twice per day for 14 consecutive days. Tissue sampling was carried out on days 4, 7, 10, and 14 post wounding. The histological parameters including inflammatory cells infiltration, fibroblast proliferation, re-epithelialization, granulation formation, and collagen formation were evaluated in all studied time points. Results: Compared to the control group, CNSs showed significant wound healing activities with lower inflammatory cells infiltration, higher fibroblastosis and new epithelium thickness, and greater granulation area and collagen fibers density in the ulcer bed. In addition, honey synergistically increased the wound healing activity of the studied nanoparticles. Conclusion: These results showed that CNSs have promising wound healing activity specially when dissolved with honey concurrently.
LRG1 Accelerates Wound Healing in Diabetic Rats by Promoting Angiogenesis via the Wnt/β-Catenin Signaling Pathway
Authors: Yang Ping , Li Shiyan , Zhang Hao , Ding Xiaofeng , Tan Qian
Published: February 2022

Patients with diabetic wounds may end with lower extremity amputation or death. Leucine-rich α-2-glycoprotein 1 (LRG1) is an effective regulator of angiogenesis and essential for timely wound healing. However, its role in regulating angiogenesis in diabetic wounds remains unclear. This study aimed to investigate the pro-angiogenic function of exogenous LRG1 in diabetic wound healing and explore possible mechanisms. LRG1 expression patterns following injury in normal and diabetic wounds were determined by western blotting. Local injection of LRG1 was used to verify the effects on angiogenesis and wound healing in diabetic rats. Immunohistochemical staining for CD31 was used to analyze the vessel density. Human umbilical vein endothelial cells (HUVECs) cultured in hyperglycemia were used to explore how LRG1 promotes angiogenesis in diabetic wound healing. We found that the expression peak of LRG1 around the wounds was delayed in diabetic rats compared with that in normal rats. Exogenous administration of LRG1 significantly accelerated the wound closure rate and promoted angiogenesis in diabetic rats. In addition, exogenous LRG1 effectively restored the proliferation, migration, and tube formation ability of HUVECs under hyperglycemia. Mechanistically, LRG1 promoted angiogenesis and diabetic wound healing mainly by activating the Wnt/β-catenin pathway, which is inhibited in diabetic wounds. This research suggests that LRG1 promotes angiogenesis and wound closure in diabetic rats by improving angiogenesis via activation of the Wnt/β-catenin pathway. Hence, LRG1 may be a possible therapeutic strategy for diabetic foot treatment.
Faculty Members’ Perceptions and Attitudes Towards Anti-Plagiarism Detection Tools: Applying the Theory of Planned Behavior
Authors: Arabyat Rasha M. , Qawasmeh Bashar R. , Al-Azzam Sayer I. , Nusair Mohammad B. , Alzoubi Karem H.
Published: February 2022

Objective

To evaluate predictors of the use of anti-plagiarism detection tools (APTs) in higher education institutions.

Methods

An online survey based on the theory of planned behavior was sent to members of science faculties in Jordan. Multivariate Logistic regression was conducted to determine predictors of previous use of APTs, and multivariate linear regression to predict future intentions.

Results

A total of 173 faculty members completed the survey, with 78.13% indicating previous APTs use. Turnitin® (43%) and Ithenticate® (32.8%) were the most commonly used APTs. These tools were mainly used to evaluate papers before sending them to journals (65.5%) and to detect plagiarism in theses/dissertations (50.4%). Predictors of previous use were academic rank (p  =  0.02) and perceived understanding of APT use (p  =  0.043). Intentions for future use were predicted by attitudes (p < 0.001) and perceived behavioral control (p  =  0.036).

Conclusion

The theory of planned behavior successfully predicted intentions to use APTs. The use of APTs should be encouraged to all faculty members and students.
False friends: Leftist nationalism and the project of transnational solidarity
Authors: Anderl Felix
Published: February 2022

A growing number of left-wing scholars criticize practices of transnational solidarity. Pointing to the cooptation of “globalism” by neoliberal capitalism, these scholars utilize this critique to advance leftwing nationalism. In this article, I reconstruct symptomatic texts of this genre and identify the critique of (liberal) cosmopolitanism as the common denominator in their calls for nationalizing the Left. As a consequence of their opposition to cosmopolitanism, these authors reject freedom of movement or global justice activism. In order to examine whether the project of transnational solidarity is affected by this critique, I reconstruct its justifications in Critical Theory and postcolonial-feminist theory. Hauke Brunkhorst and Chandra Mohanty exemplarily theorize transnational solidarity in different ways, but each based on a substantial critique of liberal cosmopolitanism. By that, I show that a principled return to nationalism derived from a critique of cosmopolitanism is informed by coincidental evidence that rejects practices of transnational solidarity because of their alleged embeddedness in cosmopolitan reason. Drawing on La Via Campesina, I illustrate principles of a transnational solidarity project that is not justified with liberal cosmopolitan ideas. Their experiences challenge the nationalist/statist critique while not falling back into naïve and/or neoliberal cosmopolitanism.
Predicting response to neoadjuvant chemoradiotherapy in rectal cancer: from biomarkers to tumor models
Authors: Li Moying , Xiao Qiyun , Venkatachalam Nachiyappan , Hofheinz Ralf-Dieter , Veldwijk Marlon R. , Herskind Carsten , Ebert Matthias P. , Zhan Tianzuo
Published: February 2022

Colorectal cancer (CRC) is a major contributor to cancer-associated morbidity worldwide and over one-third of CRC is located in the rectum. Neoadjuvant chemoradiotherapy (nCRT) followed by surgical resection is commonly applied to treat locally advanced rectal cancer (LARC). In this review, we summarize current and novel concepts of neoadjuvant therapy for LARC such as total neoadjuvant therapy and describe how these developments impact treatment response. Moreover, as response to nCRT is highly divergent in rectal cancers, we discuss the role of potential predictive biomarkers. We review recent advances in biomarker discovery, from a clinical as well as a histopathological and molecular perspective. Furthermore, the role of emerging predictive biomarkers derived from the tumor environment such as immune cell composition and gut microbiome is presented. Finally, we describe how different tumor models such as patient-derived cancer organoids are used to identify novel predictive biomarkers for chemoradiotherapy (CRT) in rectal cancer.
Antithrombotic Effect of Sorbus commixta in an Arterial Thrombosis Mouse Model
Authors: Kim Mikyung , Park Jung-Min , Kim Young-Mi , Chin Young-Won , Lee Moo-Yeol
Published: February 2022

Sorbus commixta Hedlund ( S. commixta) is known to possess cardiovascular protective activity. However, its effect on thrombosis has not been reported, although thrombosis plays a primary role in diverse circulatory diseases. Thus, this study was performed to assess the antithrombotic activity of dried S. commixta fruit extract. Mice were fed with a diet containing 8.3% extract for 4 weeks, which corresponded to the consumption of approximately 1000 mg extract/kg/day. The antithrombotic effect was tested in a FeCl3-induced arterial thrombosis model. Additionally, the effects on platelet aggregation and plasma coagulation were examined in vitro using washed platelets (WP) and isolated blood plasma, respectively. Thrombotic vascular occlusion time was significantly prolonged by the 4-week administration of the extract. Treatment of WP with the extract resulted in a decrease in thrombin-induced aggregation, which was concentration-dependent over 10 to 100 μg/mL and was not attributed to the direct inhibition of thrombin. The extract did not affect blood plasma coagulation, which was assessed by measuring prothrombin time and activated partial thromboplastin time. S. commixta is capable of preventing arterial thrombosis and such an effect may be attributed to the inhibition of platelet aggregation.
Critical infections in the head and neck: A pictorial review of acute presentations and complications
Authors: Shareef Muhammed , Ghosn Youssef , Khdhir Mihran , El Annan Tamara , Alam Raquelle , Hourani Roula
Published: February 2022

Non-traumatic head and neck emergencies include several disease processes such as infectious, inflammatory, and malignant. Infections are among the most common pathological processes that affect the head and neck, and are particularly important due to their acute, severe, and potentially life-threatening nature. Radiologists need to be well acquainted with these entities because any delay or misdiagnosis can lead to significant morbidity and mortality. Having a general understanding of such diseases is crucial, their prevalence, clinical presentation, common causative pathogens, route of spread, potential complications, and multimodality radiological appearance. Furthermore, understanding the relevant anatomy of the region, including the various fascial planes and spaces, is essential for radiologists for accurate image interpretation and assessment of potential complications. Our aim is to review the most common severe infections affecting the head and neck as well as other rare but potentially life-threatening infections. We will also describe their imaging features while focusing on the anatomy of the regions involved and describing their potential complications and treatment options.
It's the Theory, Stupid
Authors: Aguinis Herman , Cronin Matthew A.
Published: February 2022

To the complex question of “What is the number one issue on which we should focus as producers, evaluators, and consumers of research?” our simple and blunt answer is: It's the theory, stupid. Accordingly, we offer guidance on how to produce, test, and use theory by answering the following questions: (1) Why is theory so critical and for whom? (2) What does a good theory look like? (3) What does it mean to have too much or too many theories? (4) When don’t we need a theory? (5) How does falsification work with theory? and (6) Is good theory compatible with current publication pressures? Our answers are useful to current and future scholars and journal editors and reviewers, as well as consumers of research including other researchers, organization decision makers, and policy makers, and other stakeholders in the theory production and testing process including deans and other university administrators.
Acceptance and use of telemedicine technology by health professionals: Development of a conceptual model
Authors: Rouidi Mohammed , Elouadi Abdelmajid , Hamdoune Amine
Published: February 2022

Recent developments in information technology (IT) in health are extended to highly specialized services, an example is telemedicine technology, understood as the use of IT to enable the transfer of medical information for diagnostic purposes, therapeutic and educational. Despite the benefits of implementing such technology, healthcare professionals, as end users, do not fully utilize it. The Technology Acceptance Model (TAM) and the Unified Theory of Acceptance and Use of Technology (UTAUT), are among the models applied to assess and predict the acceptance and use of telemedicine. This article aims to identify the relevant literature related to these two models, to review and summarize the methodologies and results, and propose a conceptual model for the acceptance and use of telemedicine technology by healthcare professionals.
A Community Health Volunteer Involvement Program for Glycated Hemoglobin Reduction Among Thai Patients With Uncontrolled Type 2 Diabetes: A Mixed-Method Study
Authors: Sittipreechachan Penchan , Pichayapinyo Panan , Lagampan Sunee , Chongsuwat Rewadee
Published: February 2022

Background:

Diabetes mellitus is increasing and a shortage exists of nurses to care for patients. Community health volunteers (CHVs) pose potential supportive networks in assisting patients to perform healthy behaviors.

Aim:

The study aimed to develop and investigate the effects of a CHV involvement program on reducing glycated hemoglobin (HbA1c) levels among Thai patients with uncontrolled type 2 diabetes.

Methods:

This sequential mixed-method study was conducted from January to June 2019. Sixty patients with HbA1c exceeding 7% were recruited from 2 communities assigned as the intervention and comparison groups. Using King’s General Systems Framework as a basis to develop the program, the study initially explored the perceptions of diabetes and its management among patients, family members, and CHVs. Then, a quasi-experimental study with 2 groups pretest-posttest design was conducted and compared with usual care. The intervention included educational sessions, home visits, and activities created by CHVs including a campaign, broadcasting, and health food shops. Quantitative data were collected at baseline and 20-week follow-up and analyzed by descriptive statistics, Independent t-test, and paired t-test.

Results:

The intervention group exhibited a lower mean HbA1c ( p < .001) and reported significant, improvement concerning diabetes knowledge, self-efficacy, perceived support, and behavior compared with the comparison group at the end of the study (Cohen’s d > 1.0, effect size large).

Conclusion:

Applying this framework to develop the program could benefit glycemic control among patients with uncontrolled diabetes residing in communities. Further studies should be conducted on a large sample to demonstrate the efficacy of the program.
The Impacts of Drug and Alcohol use on Sentencing for First Nations and Non-Indigenous Defendants
Authors: Velazquez Marisela , Petray Theresa L. , Miles Debra
Published: February 2022

This paper examines the ways personal use of illicit substances and alcohol are constructed as either mitigating or aggravating factors to explain offending. We consider the differential constructions of these factors for people who appear in supreme and district courts in northern Queensland, Australia, for offences involving illicit substance use, alcohol use, drug-related offences, and violence. Qualitative analysis of courtroom observations is understood through the lens of Critical Race Theory (CRT). Our findings reveal that personal use of illicit substances was primarily constructed by legal practitioners as an indicator of disadvantaged circumstances when discussing non-Indigenous defendants. In these cases, drug use was connected to other disadvantages such as poor mental health, physical pain, and trauma. In contrast, alcohol use was primarily raised as an aggravating factor for First Nations defendants, constructed by legal practitioners as a personal flaw linked to violent offending, and overshadowed the interrelated disadvantages that many First Nations defendants experience. This reflects social attitudes about First Nations people, reinforces individualistic explanations for offending patterns, and points to the institutional racism embedded in the structural processes of Queensland's higher courts that continues to profoundly impact First Nations people.
Evaluating YouTube Video Quality in Orthognathic Surgery Patient Education
Authors: Puthumana Joseph S. , Lopez Christopher D. , Girard Alisa , Lake Isabel V. , Taylor Ainsley L. , Khoo Kimberly H. , Rottgers Alex , Yang Robin , Halsey Jordan
Published: February 2022

Background: Patients are increasingly accessing consumer-style health information from a variety of web-based platforms. This study aims to assess a popular audio-visual platform for its quality of information in orthognathic procedures. Methods: We queried YouTube on August 12th, 2021 for 20 videos, by both relevance and view count, under each of 3 search terms: (1) “orthognathic surgery,” (2) “LeFort I osteotomy,” and (3) “sagittal split osteotomy.” Each video was characterized by date of upload, time since upload, video duration, video type, and video author. Videos were further characterized by type of video (1) creator and (2) category. The provider-validated instrument DISCERN was applied to each video to assess content quality and bias. Results: Of 60 videos marked for review, 46 were included for analysis. The most common category of video was operative ( n = 18, 36.1%), while the most common creator type was non-physician medical professional ( n = 14, 30.4%). Hospital or physician advertisement videos had the greatest video power index (26 297 ± 44 556), while medical education videos had the least (13 ± 9). Significant differences were found across both video type and video creator in viewership ( P = .008 and .003, respectively) and video power index ( P = .010 and .007), but not duration ( P = .796 and .059). DISCERN scores ranged from 16 to 80 and were subdivided into 5 categories: very poor (16-28), poor (29-41), fair (42-54), good (55-67), and excellent (68-80). Average DISCERN scores for all 46 videos were 17.9 ± 4.9 for reliability, 14.2 ± 4.0 for quality, and 34.3 ± 9.0 for overall. Conclusions: YouTube videos on orthognathic surgery were of overall poor quality. These videos were rated best in relevance to the search parameter and description of treatment but were least helpful in describing alternatives to the treatment and uncertainties about the procedure. Videos created by patients and about patient experiences were rated best for content that was reliable, high quality, and low bias.
Structural basis of lignocellulose deconstruction by the wood-feeding anobiid beetle Nicobium hirtum
Authors: Krishanti Ni Putu Ratna Ayu , Tobimatsu Yuki , Miyamoto Takuji , Fujimoto Izumi , Kartika Titik , Umezawa Toshiaki , Hata Toshimitsu , Yoshimura Tsuyoshi
Published: February 2022

The details of the lignocellulose deconstruction processes in the digestive systems of wood-feeding insects remain elusive. This study aimed to examine the biochemical conversion of lignocellulose in the digestive system of a wood-feeding anobiid beetle, Nicobium hirtum, one of the most important pests of wooden products in Japan. To this end, N. hirtum larvae were fed with Japanese red pine (softwood) and Japanese beech (hardwood) sapwood diets, as well as an artificial diet containing Shorea wood (hardwood) sapwood sawdust. The structural differences between the original and digested (feces) lignocellulose samples were examined using wet-chemical and two-dimensional (2D) nuclear magnetic resonance (NMR) methods. Cellulose and hemicelluloses, especially mannan in the softwood diet, were preferentially degraded over lignin in the larval digestive system. As a result, lignin was enriched in the digested lignocellulose residues. Lignin compositional analyses based on thioacidolysis and 2D NMR determined that the proportions of oxidized lignin aromatic units were notably increased after digestion. Further, the 2D NMR analyses revealed the accumulation of aldehyde and hydroxypropiovanillone/syringone end-unit structures in lignin, indicating that oxidative and/or reductive modifications of lignin polymers occur in the larval digestive system. Such structural alterations of lignin may facilitate the dissociation of the lignin barrier, thereby liberating polysaccharides for subsequent enzymatic conversion for assimilation and energy.
Prospective and retrospective performance assessment of Advanced Driver Assistance Systems in imminent collision scenarios: the CMI-Vr approach
Authors: Gulino Michelangelo S. , Fiorentino Anita , Vangi Dario
Published: February 2022

Structured abstract

Introduction

Prospective and retrospective performance assessment of Advanced Driver Assistance Systems (ADASs) is fundamental to pilot future enhancements for active safety devices. In critical road scenarios between two vehicles where ADAS activation enables collision mitigation only, currently available assessment methodologies rely on the reconstruction of the impact phase consequent to the specific intervention on braking and steering: the velocity change sustained by the vehicle in the collision ($$\Delta V$$

Δ
V

) is retrieved, so that IR decrease for the vehicle occupants can be obtained by appropriate Injury Risk (IR) models. However, information regarding the ADAS performance is available only after the impact phase reconstruction and not just as when the criticality occurs in the pre-impact phase: the best braking and steering alternative cannot be immediately envisaged, since a direct correlation lacks between the braking/steering intervention and IR.

Method

This work highlights an ADAS performance assessment method based on the disaggregation of $$\Delta V$$

Δ
V

in the two pre-impact parameters closing velocity at collision ($$V_r$$

V
r

) and impact eccentricity, represented by the Crash Momentum Index (CMI). Such a disaggregation leads to the determination of IR based solely on impact configuration between the vehicles, without directly considering the impact phase. The performance of diverse ADASs in terms of intervention logic are directly comparable based on the resulting impact configuration, associated with a single coordinate in the CMI-$$V_r$$

V
r

plane and a sole IR value as a consequence.

Results

The CMI-$$V_r$$

V
r

approach is employable for both purposes of prospective and retrospective performance assessment of ADAS devices. To illustrate the advantages of the methodology, a solution for prospective assessment based on the CMI-$$V_r$$

V
r

plane is initially proposed and applied to case studies: this provides direct suggestions regarding the most appropriate interventions on braking and steering for IR minimization, fundamental in the tuning or development phase of an ADAS. A method for retrospective assessment is ultimately contextualized in the EuroNCAP “Car-to-Car Rear moving” test for an Inter-Urban Autonomous Emergency Braking system, a device implemented on a significant portion of the circulating fleet.

Conclusions

Based on the evidenced highlights, it is demonstrated that the approach provides complementary information compared to well-established performance assessment methodologies in all stages of an ADAS life cycle, by suggesting a direct physical connection in the pre-impact phase between the possible ADAS interventions and the foreseeable injury outcomes.
Genome-wide investigation of the ZF-HD gene family in two varieties of alfalfa (Medicago sativa L.) and its expression pattern under alkaline stress
Authors: He Kai , Li Chunxin , Zhang Zhenyue , Zhan Lifeng , Cong Chunlong , Zhang Depeng , Cai Hua
Published: February 2022

Background

Zinc finger homeodomain (ZHD) protein is a plant-specific transcription factor and a potential regulator of phosphoenolpyruvate carboxylase (PEPCase)-coding genes, and it also participates in plant growth regulation and abiotic stress responses. To study the function of MsZF-HD genes in the alkaline stress response, this paper assessed biological information and performed transcriptome analysis of the MsZF-HD gene family by using the genomes of two different varieties of alfalfa (XinJiangDa Ye and Zhongmu No. 1).

Results

In total, 49 and 11 MsZF-HD genes were identified in the two different varieties respectively, including the alleles of XinJiangDa Ye. According to their phylogenetic relationships, the 60 MsZF-HD genes were divided into 5 ZHD subfamilies and 1 MIF subfamily. A total of 88.3% of MsZF-HD genes do not contain introns and are unevenly distributed among the 6 chromosomes of alfalfa. A collinearity analysis indicated that 26 genes of XinJiangDa Ye have no orthologous genes in Zhongmu No. 1, although these genes (such as ZHD-X1–2, ZHD-X3–2 and ZHD-X4–2) have homologous genes in Arabidopsis thaliana, Medicago truncatula and Glycine max. Through RNA-seq and qRT–PCR verification, it was found that MsZF-HD genes are downregulated to participate in the alkaline stress response.

Conclusion

The results of this study may lay the foundation for the cloning and functional study of MsZF-HD genes and provide a theoretical basis for revealing the difference between XinJiangDa Ye and Zhongmu No. 1 at the genome level.
Transcriptomic comparison of ovarian granulosa cells between adult sheep and prepubertal lambs
Authors: Tian Hao , Ren Panyu , Liu Kailing , Qiu Chunjuan , Fan Lihong , Li Junlong , Hou Jian
Published: February 2022

Background

The oocyte development ability of prepubertal animals is significantly lower than that of adult animals. Granulosa cells (GCs) have an important function on regulation of follicular and oocyte development. Therefore, analysis of GC characteristics can be used to explore the developmental mechanism of follicles and oocytes.

Results

In order to understand the possible reasons for the differences in follicle and oocyte development between lambs and adult sheep, we utilized high-throughput sequencing technique to analyze the transcriptome of GCs from follicle-stimulating hormone (FSH) superstimulated adult ewes and prepubertal lambs. Adult ewes were treated with FSH for 3 days (group A) and lambs were FSH-treated for 2 days (group B) or 3 days (group C). Transcriptome analysis of GCs showed that there were 405 and 159 differentially expressed genes from A vs. B and A vs. C, respectively. The results indicated that prolonging the FSH-treatment of lambs made the GC state of lambs more similar to the adult ewes, but there were still a large number of differentially expressed genes between adult ewes and lambs. Further analysis showed that many differently expressed genes were implicated in cell proliferation and apoptosis, oocyte development and follicular ovulation. Cellular examination demonstrated that fatty acid binding protein 4 (FABP4), which was highly expressed in lamb GCs, had a potential of promoting cell apoptosis. Cytoplasmic phospholipase A2 (PLA2G4A), which was expressed lowly in lamb GCs, may be responsible for reduced synthesis of prostaglandins in cells and impaired follicle/oocyte development. In contrast, glutathione S-transferase β-1 (GSTT2B) and forkhead boxO6 (FOXO6) had no apparent effect on the proliferation and apoptosis of GCs.

Conclusions

Our study found dramatic transcriptomic differences in GCs between lambs and adult sheep, which may explain the possible reasons for the defects of follicle and oocyte development in lambs compared to adult sheep. Our data provides important information for further understanding the mechanism of follicular development in prepubertal animals and improving their oocyte developmental competence.
Biocontrol properties from phyllospheric bacteria isolated from Solanum lycopersicum and Lactuca sativa and genome mining of antimicrobial gene clusters
Authors: Muñoz Claudia Y. , Zhou Lu , Yi Yunhai , Kuipers Oscar P.
Published: February 2022

Background

Biocontrol agents are sustainable eco-friendly alternatives for chemical pesticides that cause adverse effects in the environment and toxicity in animals including humans. An improved understanding of the phyllosphere microbiology is of vital importance for biocontrol development. Most studies have been directed towards beneficial plant-microbe interactions and ignore the pathogens that might affect humans when consuming vegetables. In this study we extended this perspective and investigated potential biocontrol strains isolated from tomato and lettuce phyllosphere that can promote plant growth and potentially antagonize human pathogens as well as plant pathogens. Subsequently, we mined into their genomes for discovery of antimicrobial biosynthetic gene clusters (BGCs), that will be further characterized.

Results

The antimicrobial activity of 69 newly isolated strains from a healthy tomato and lettuce phyllosphere against several plant and human pathogens was screened. Three strains with the highest antimicrobial activity were selected and characterized (Bacillus subtilisSTRP31,Bacillus velezensisSPL51, andPaenibacillussp.PL91). All three strains showed a plant growth promotion effect on tomato and lettuce. In addition, genome mining of the selected isolates showed the presence of a large variety of biosynthetic gene clusters. A total of 35 BGCs were identified, of which several are already known, but also some putative novel ones were identified. Further analysis revealed that among the novel BGCs, one previously unidentified NRPS and two bacteriocins are encoded, the gene clusters of which were analyzed in more depth.

Conclusions

Three recently isolated strains of the Bacillus genus were identified that have high antagonistic activity against lettuce and tomato plant pathogens. Known and unknown antimicrobial BGCs were identified in these antagonistic bacterial isolates, indicating their potential to be used as biocontrol agents. Our study serves as a strong incentive for subsequent purification and characterization of novel antimicrobial compounds that are important for biocontrol.
Perceptions on barriers, facilitators, and recommendations related to mental health service delivery during the COVID-19 pandemic in Quebec, Canada: a qualitative descriptive study
Authors: Spagnolo Jessica , Beauséjour Marie , Fleury Marie-Josée , Clément Jean-François , Gamache Claire , Sauvé Carine , Couture Lyne , Fleet Richard , Knight Shane , Gilbert Christine , Vasiliadis Helen-Maria
Published: February 2022

Background

There was an increase in self-reported mental health needs during the COVID-19 pandemic in Canada, with research showing reduced access to mental health services in comparison to pre-pandemic levels. This paper explores 1) barriers and facilitating factors associated with mental health service delivery via primary care settings during the first two pandemic waves in Quebec, Canada, and 2) recommendations to addressing these barriers.

Methods

A qualitative descriptive study design was used. Semi-structured interviews with 20 participants (health managers, family physicians, mental health clinicians) were conducted and coded using a thematic analysis approach.

Results

Barriers and facilitating factors were organized according to Chaudoir et al. (2013)‘s framework of structural, organizational, provider- and patient-related, as well as innovation (technological modalities for service delivery) categories. Barriers included relocation of mental health staff to non-mental health related COVID-19 tasks (structural); mental health service interruption (organizational); mental health staff on preventive/medical leave (provider); the pandemic’s effect on consultations (i.e., perceptions of increased demand) (patients); and challenges with the use of technological modalities (innovation). Facilitating factors included reinforcements to mental health care teams (structural); perceptions of reductions in wait times for mental health evaluations during the second wave due to diminished FP referrals in the first wave, as well as supports (i.e., management, private sector, mental health trained staff) for mental health service delivery (organizational); staff’s mental health consultation practices (provider); and advantages in increasing the use of technological modalities in practice (innovation).

Conclusions

To our knowledge, this is the first study to explore barriers and facilitating factors to mental health service delivery during the pandemic in Quebec, Canada. Some barriers identified were caused by the pandemic, such as the relocation of staff to non-mental health services and mental health service interruption. Offering services virtually seemed to facilitate mental health service delivery only for certain population groups. Recommendations related to building and strengthening human and technological capacity during the pandemic can inform mental health practices and policies to improve mental health service delivery in primary care settings and access to mental health services via access points.
The long-term prognostic value of serum 25(OH)D, albumin, and LL-37 levels in acute respiratory diseases among older adults
Authors: Aronen Matti , Viikari Laura , Langen Henriikka , Kohonen Ia , Wuorela Maarit , Vuorinen Tytti , Söderlund-Venermo Maria , Viitanen Matti , Camargo Carlos Arturo , Vahlberg Tero , Jartti Tuomas
Published: February 2022

Background

Older adults are more susceptible to respiratory tract infection than healthy working age adults. The increased susceptibility of older adults is thought to be interlinked with vitamin D status, nourishment, and immunological state in general. Data are scarce whether these parameters could serve as prognostic markers.

Aim

To study whether serum 25(OH)D, albumin, and LL-37 level could give prognostic value of long-term survival in the older adults with multimorbidity and acute respiratory infection.

Methods

Consecutive episodes of hospital care of patients 65 years and older with respiratory symptoms were prospectively studied as a cohort. Standard clinical questionnaire was filled by the study physician. Laboratory markers included serum levels of 25(OH)D, albumin and LL-37, C-reactive protein (CRP), white blood cell count (WBC) and polymerase chain reaction diagnostics for 14 respiratory viruses. Pneumonia was confirmed by chest radiographs. Respiratory illness severity, death at ward, length of hospital stays, and 5-year survival were used as outcomes.

Results

In total, 289 older adult patients with mean age of 83 years were included in the study. Serum 25(OH)D deficiency (< 50 nmol/liter) was present in 59% and hypoalbuminemia (< 3.5 g/dL) in 55% of the study patients. Low serum albumin level was associated to one, two- and five-year mortality after hospital stay (all P < .05). In addition, it was associated with pneumonia, dyspnea, over 13-night long stay at ward and death at ward (all P < .05). No associations were seen between serum 25(OH)D and LL-37 levels and disease severity, short-term clinical outcome, or long-term survival. Associations between serum 25(OH)D, albumin, and LL-37 levels and respiratory virus presence were not seen.

Conclusions

Serum albumin level on admission seems to give valuable information about the patients’ general health and recovery potential in treating older adults with respiratory symptoms. Serum 25(OH)D and LL-37 had no associations with disease severity or long- and short-term prognosis among older adults hospitalized with respiratory symptoms.
Multi-body-site colonization screening cultures for predicting multi-drug resistant Gram-negative and Gram-positive bacteremia in hematological patients
Authors: Torres Ignacio , Huntley Dixie , Tormo Mar , Calabuig Marisa , Hernández-Boluda Juan Carlos , Terol María José , Carretero Carlos , de Michelena Paula , Pérez Ariadna , Piñana José Luis , Colomina Javier , Solano Carlos , Navarro David
Published: February 2022

Background

To investigate the multi-drug resistant bacteria (MDRB) colonization rate in hematological patients hospitalized for any cause using a multi-body-site surveillance approach, and determine the extent to which this screening strategy helped anticipate MDRB bloodstream infections (BSI).

Methods

Single-center retrospective observational study including 361 admissions documented in 250 adult patients. Surveillance cultures of nasal, pharyngeal, axillary and rectal specimens (the latter two combined) were performed at admission and subsequently on a weekly basis. Blood culture samples were incubated in an automated continuous monitoring blood culturing instrument (BACTEC FX).

Results

In total, 3463 surveillance cultures were performed (pharyngeal, n = 1201; axillary-rectal, n = 1200; nasal, n = 1062). MDRB colonization was documented in 122 out of 361 (33.7%) admissions corresponding to 86 patients (34.4%). A total of 149 MDRB were isolated from one or more body sites, of which most were Gram-negative bacteria, most frequently non-fermenting (n = 83) followed by Enterobacterales (n = 51). BSI were documented in 102 admissions (28%) involving 87 patients. Overall, the rate of BSI caused by MDRB was
significantly higher (p = 0.04) in the presence of colonizing MDRB (16 out of 47 admissions in 14 patients) than in its absence (9 out of 55 admissions in 9 patients). Colonization by any MDRB was independently associated with increased risk of MDRB-BSI (HR, 3.70; 95% CI, 1.38–9.90; p = 0.009).

Conclusion

MDRB colonization is a frequent event in hematological patients hospitalized for any reason and is associated with an increased risk of MDRB BSI. The data lend support to the use of MDRB colonization surveillance cultures for predicting the occurrence of MDRB BSI in this cohort.
Gait disorders in CKD patients: muscle wasting or cognitive impairment? A cross-sectional pilot study to investigate gait signatures in Stage 1–5 CKD patients
Authors: Zemp Damiano D. , Giannini Olivier , Quadri Pierluigi , Rabuffetti Marco , Tettamanti Mauro , de Bruin Eling D.
Published: February 2022

Background

Instrumental gait analysis in nephrology is widely neglected, although patients with chronic kidney disease (CKD) show brain changes due to cerebrovascular disease and metabolic disorders that can potentially influence gait quality. Our study assesses the association between CKD stages and gait parameters, to understand the prevalent status of brain related gait parameters (i.e. variability) and of performance related parameters (i.e. gait speed, stride length). We hypothesize that gait changes are detectable already in early stages of CKD.

Methods

Forty-five participants distributed in 5 CKD severity groups underwent an instrumental gait analysis via a triaxial accelerometer affixed to the lower trunk under single- and dual-task conditions. In addition to spatio-temporal parameters, variability and dual-task cost of gait were extracted. A battery of clinical assessments was conducted with the aim of helping to better explain the findings of the gait analysis. A correlation analysis was made to investigate a linear relation between gait parameters and CKD severity.

Results

Statistically significant correlations (Pearson correlation coefficient) with CKD severity were found for gait speed (p < 0.01, r = -0.55, 95% CI [-0.73;-0.30]), stride length ( p < 0.01, r = -0.40, 95% CI [-0.62;-0.12]), step length (p < 0.01, r = -0.41, 95% CI [-0.63;-0.13], coefficient of variance (CV) of step length (p = 0.01, r = 0.36, 95% CI [0.08;0.59]), gait regularity (p < 0.01, r = -0.38, 95% CI [-0.61;-0.10]), dual-task cost of gait speed (p < 0.01, r = 0.40, 95% CI [0.13;0.62]) and dual-task cost of stride time (p = 0.03, r = 0.32, 95% CI [0.03;0.57]). Adjustment for age and gender confirmed all results except for gait regularity. With increasing severity of renal failure, Handgrip strength, Time for the Expanded Timed Get Up and Go test, executive functions, haemoglobin, and haematocrit, worsen.

Conclusions

The correlation of CKD severity with spatio-temporal parameters (performance indices mainly relatable to peripheral functionality) and with variability of gait (related to central factors) supported by the results of the clinical assessments, suggests that gait disturbance in CKD patients is not only due to metabolic factors that lead to muscle wasting, but also to brain changes that affect motor control. This suggests that the treatment of renal disease should include cognitive aspects in addition to metabolic and functional factors.
Obstetric outcomes in pregnant COVID-19 women: the imbalance of von Willebrand factor and ADAMTS13 axis
Authors: Grandone Elvira , Vimercati Antonella , Sorrentino Felice , Colaizzo Donatella , Ostuni Angelo , Ceci Oronzo , Capozza Manuela , Tiscia Giovanni , De Laurenzo Antonio , Mastroianno Mario , Cappucci Filomena , Fischetti Lucia , Margaglione Maurizio , Cicinelli Ettore , Nappi Luigi
Published: February 2022

Background

Thrombotic microangiopathy has been invoked as one of the most important mechanisms of damage in COVID-19 patients. Protease ADAMTS13 is a marker of microangiopathy responsible for controlling von Willebrand multimers size. Von Willebrand factor/ADAMTS13 ratio has been found impaired in COVID-19 patients outside pregnancy.

Methods

We prospectively investigated 90 pregnant women admitted to two tertiary academic hospitals in Italy with a laboratory-confirmed diagnosis of SARS-CoV-2 infection. Demographic, clinical information and routine laboratory data were collected at the hospital admission and until discharge. We investigated whether vonWillebrand /ADAMTS13 axis imbalance is a predictor of adverse outcomes. Logistic regression analysis, which controlled for potential confounders, was performed to evaluate the association between laboratory parameters and clinical outcomes.

Results

Most women (55.6%) were parae, with median gestational age at admission of 39 weeks. At hospital admission, 63.3% were asymptomatic for COVID-19 and 24.4% showed more than one sign or symptom of infection. Nulliparae with group O showed Willebrand / ADA MTS-13 ratios significantly lower than non-O, whereas in multiparae this difference was not observed. Logistic regression showed that ratio von Willebrand to ADAMTS13 was significantly and independently associated with preterm delivery (OR 1.9, 95%CI 1.1–3.5).

Conclusion

This study shows an imbalance of vonWillebrand /ADAMTS13 axis in pregnant women with COVID-19, leading to a significantly higher and independent risk of preterm delivery. Monitoring these biomarkers might support decision making process to manage and follow-up pregnancies in this setting.
Clinical outcomes of advanced non-small cell lung cancer patients harboring distinct subtypes of EGFR mutations and receiving first-line tyrosine kinase inhibitors: brain metastasis and de novo T790M matters
Authors: Zeng Ya , Guo Tiantian , Zhou Yue , Zhao Yang , Chu Li , Chu Xiao , Yang Xi , Ni Jianjiao , Zhu Zhengfei
Published: February 2022

Background

The clinical features, survival outcomes and patterns of treatment failure of advanced non-small cell lung cancer (NSCLC) patients harboring distinct subtypes of EGFR mutations and receiving first-line EGFR tyrosine kinases inhibitor (TKIs) are not fully understood.

Methods

Consecutive metastatic EGFR-mutant NSCLC patients receiving first-line EGFR-TKIs from October 2010 to March 2020 were enrolled and classified into two main groups based on the EGFR mutation subtypes: common mutation (L858R or exon 19 deletion), uncommon mutation (other EGFR mutations).

Results

Of the 1081 patients included, 74 (6.8%) harbored uncommon mutations. The baseline characteristics were generally balanced between the two groups, except that bone metastasis developed less frequently in patients with uncommon mutations (p = 0.02). No significant difference of survival outcomes was found between the two groups, except that among patients with baseline brain metastasis, the intracranial time to progression was significantly shorter in patients with uncommon mutations. Nine of the 17 patients with de novo T790M mutation received Osimertinib, whose overall survival tended to be longer than the remaining 8 patients without Osimertinib treatment (p = 0.08). The patterns of treatment failure were generally consistent between the two groups, except which patients with uncommon mutations had a higher risk developing progressive disease in the brain.

Conclusion

First-line EGFR-TKIs seemed to be less effective in controlling and preventing brain metastasis in patients with uncommon EGFR mutations and Osimertinib was associated with promising efficacy in patients with de novo T790M mutation, which warranted further validation.