bluuebunny/crossref_metadata_2025 huggingface dataset:
Fotocatálise do Corante Azul de Metileno por Sistemas como SrSnO 3, BaSrSnO3 e CaSrSnO3
Authors: Oliveira Letícia G. Oliveira , Rodrigues Murillo H. M. , Eduardo Ariadne C. , Godinho Mario J. , Santos Maria R. de C.
Published: March 2020

Com a grande preocupação da população com os problemas ambientais, têm surgido métodos mais efiazes para o tratamento de eflentes. Nesse contexto, surge o estudo sobre a aplicação dos materiais cerâmicos, por exibir propriedades fotocatalíticas como uma alternativa para a descontaminação de corantes em águas residuais. Este trabalho, reportam-se dados da degradação fotocatalítica do corante Azul de Metileno após a adição do catalisador SnSrO 3 (antes e após a adição de 0,1% Ba e 0,1% Ca). Para tal, amostras foram sintetizados pelo método do Precursor Polimérico, calcinadas à: 500, 800 e 1000 ºC/4 horas e caracterizadas por DRX, UV-VIS e fotocatálise.
Electrolytes Profile of Critically Ill Patients Admitted To Pediatric Intensive Care Unit (PICU) Dr. Soetomo General Hospital
Authors: Octavia Amalia Citra , Setyaningtyas Arina , Edwar Pesta Parulian Maurid
Published: March 2020

Introduction: Fluid and electrolyte disorders can be affected by various conditions or diseases. Electrolyte disorders are often found in pediatric patients with critically ill conditions and are associated with increased morbidity also mortality that requires extra care in the Pediatric Intensive Care Unit (PICU). Objective: To describe the electrolyte profile of critically ill patients with electrolyte disorders admitted to the Pediatric Intensive Care Unit (PICU) Dr. Soetomo General Hospital. Materials and Methods: The study was conducted prospectively with a descriptive method in patients with electrolyte disorders aged 1 month – 18 years old admitted to PICU Dr. Soetomo General Hospital, Surabaya in a period from August to November 2018. Data on patient age, gender, electrolyte profile (sodium, potassium, calcium, chloride), and origin before PICU admission were recorded. Results and Discussion: From 37 patients with electrolyte disorders showed that patients were dominated by the male in 56.8% (n=21), in the age group of infants or 1-12 months old in 45.9% (n=17). Common main diagnosis in most patients were digestive system disorders in 27% (n=10), followed by central nervous system, respiratory system, kidney and cardiovascular disorders, and the origin before PICU admission were from Emergency Room (ER) in 62.2% (n=23). There were found 97 incidences of electrolyte disorders. Most frequent electrolyte disorders were hypocalcemia in 59.5% (n=22), hypokalemia in 54.1% (n=20), hyponatremia in 40.5% (n=15) and the least was hypochloremia in 35.1% (n=13). Mean serum sodium level was 138.18 ± 12.071, serum potassium level was 3.608 ± 1.2247, serum calcium level was 8.057 ± 1.9473 and serum chloride level was 101.45 ± 13.266. Conclusions:  Critically ill patients admitted in PICU tend to experience low electrolyte levels.
EPIDEMIOLOGICAL INVESTIGATION OF NOMA IN PAPUA PROVINCE IN 2017
Authors: Kaimudin Asrul , Hidajah Atik Choirul
Published: March 2020

Background: Indonesia Ministry of Health in October 2017 obtained information from the Public Health Emergency Operation Center that there allegations of noma case in a toddler from the Korowai tribe in Asmat district. Purpose: This study aims to describe the magnitude of the noma problem, identify risk factors, and find other noma cases for prevention and control. Methods: This was a descriptive study using a case study approach. Primary data was obtained from interviews with family or close relatives of patients to obtain information about patient identity, medical history, and risk factors. Measurement of nutritional status and oral health was done by measuring weight or height and checking their oral health. Secondary data was obtained from Asmat DHO and Yaniruma Health Center. The data analyzed were geographical, demographic, socio-cultural, transportation, and communication condition. Results: Noma sufferer was a 5-years-old child who lived in Afimabul village. There was not other cases of Noma found in Korowai tribal community. A long distance to reach health services and no availability of communication tools to Korowai tribe in Afimabul village made them inaccessible. The results of the identification of risk factors in 46 children found that the majority of them had low oral hygiene (73.91%) and consumed uncooked water (80.43%). Besides, some had an index of thin body weight (15.22%), and very lean (6.52%), and also found the suspected measles. Conclusion: There was only one case found in the Asmat district. Moreover, there were still found Korowai children  with thin and very lean nutritional status and suspected measles who are risk factors for noma.
EPIDEMIOLOGY OF DERMATITIS IN FARMERS
Authors: Yarmaliza Yarmaliza , Farisni Teungku Nih , Fitriani Fitriani , Syahputri Veni Nella , Zakiyuddin Zakiyuddin , Reynaldi Fitrah
Published: March 2020

Background: Dermatitis refers to the inflammation of the skin and can be acute, sub-acute, or chronic. In 2014, there were 364 cases in Meureubo Health Center , which increased sharply to 1,854 cases in 2915 and 2,794 cases in 2016. Purpose: This study looks at the relationship between personal hygiene and a history of contact with families suffering from dermatitis and the incidence of dermatitis in farmers in West Aceh Regency. Methods: This research was conducted using a descriptive observational design and was carried out in the working area of the Meureubo Health Center in West Aceh Regency between 5 December 2017 and 8 January 2018. A purposive sampling technique was used for the collection of primary and secondary data. Results: The majority of respondents were male (60%), aged 31–40 years (66%), and had a low education level (76%). The majority of farmers had poor personal hygiene (61%) and had a history of contact with families suffering from dermatitis (66%). Conclusion: There is a high prevalence of poor personal hygiene in farmers, and they tend to have a history of contact with family members suffering from dermatitis.
Information Technology-based Interventions for Health Care Support in Patients with Chronic Kidney Disease: A Systematic Review
Authors: Putri Inda Rian Patma , Nursalam Nursalam , Kurniawati Ninuk Dian
Published: March 2020

Background: Self-management and support in managing therapeutic regimens is very important for patients with CKD. Information technology-based interventions are increasingly being used to support the self-management of patients with CKD. This study aimed to conduct a systematic review to evaluate information technology-based interventions in relation to the support of the health management of patients with CKD.Method: We conducted a systematic review using electronic databases (Scopus, Science Direct, ProQuest) limited to the last 8 years from 2010 to 2018 with the relevant keywords. The studies included used RCT, pilot and case-control methods focusing on patients with CKD stage 1-5 and that reported on at least one outcome from the health management of patients with CKD.Result: Out of the 7.852 studies taken, 13 studies fulfilled the inclusion criteria. The interventions in this systematic review are multifaceted, including smartphone/PDA (6/13), telematics devices (2/13), internet/web (3/13) and a combination of several interventions (2 studies). In total, 12 (92%) out of the 13 studies showed a positive outcome from the intervention, 7 studies showed improved outcomes in the clinical points, 3 studies had improved adherence and 2 studies improved knowledge.Conclusion: This evidence indicates the potential of IT-based interventions (i.e. smartphone/PDA, computer, internet/web, telematic device) to support the health management of patients with CKD. The variety of interventions in this systematic review requires further research on which interventions are best applied.
The Analysis of the Influencing Factors related to the Effectiveness of Discharge Planning Implementation in Hospitals: A Systematic Review
Authors: Soebagiyo Hari , Beni Kornelis Nama , Fibriola Tan Nina
Published: March 2020

Introduction: Discharge Planning is one of the agendas that is a part of nursing duties. This is not only complicated but it is also a long-term activity, starting from when the patients are admitted to hospital until they are allowed to go home. Factually, it cannot be denied that effective nursing discharge planning implementation will provide benefits for the patient, their family and the professionals within the health care setting. Based on those facts, the author composed this systematic review with the purpose of identifying the influential factors that have a role in terms of the effectiveness of nursing discharge planning implementation in hospitalsMethods: The researcher conducted their investigation in February 2019 and this involved the exploration of scientific papers from ProQuest and Scopus using the keywords ‘nursing discharge planning’, ‘effectiveness’ and ‘implementation’. This paper identified 15 relevant research articles from 500 original full texts. These papers were analyzed according to the inclusion criteria and the impeding factors in discharge planning implementation. It involved 1 quasi-RCT and 14 descriptive research studies.Results: As a result, from the 10.000 respondents, it was found that the enhancing factors include effective communication at 43% and the factors causing impedance in nursing discharge planning effectiveness includes the continuity of staff at 38 %.Conclusion: The dominant factor of impedance as stated in the interview sessions was a lack of time to do the nursing discharge planning properly.
The Small Intestine, an Underestimated Site of SARS-CoV-2 Infection: From Red Queen Effect to Probiotics
Authors: Feng Zhijie , Wang Yan , Qi Wei
Published: March 2020

Understanding how the coronaviruses invade our body is an essential point, and the expression profile of coronaviruses receptor may help us to find where the coronavirus infects our body. We found that the coronavirus receptors, including angiotensin-converting enzyme 2 (ACE2) for SARS-CoV and SARS-Cov-2, are digestion-related enzymes in human enterocytes. Coronaviruses are continually altering the binding receptor and binding modes during their evolution, but the potential target cell in the small intestine is constant when in the lung is inconstant. Enterocytes may act as a conserved cell reservoir for coronaviruses, which may be partially explained by the Red Queen hypothesis. We also found that coronaviruses receptors could be elevated in the presence of both invasive bacteria and their counterpart, probiotics. We demonstrated here that enterocytes act as a conserved cell reservoir for coronaviruses during their evolutions, which should not be ignored in the investigation of coronavirus diagnosis and treatment strategies.
Zinc Differentially Modulates the Assembly of Soluble and Polymerized Vimentin
Authors: Mónico Andreia , Zorrilla Silvia , Rivas Germán , Pérez-Sala Dolores
Published: March 2020

The intermediate filament protein vimentin constitutes a critical sensor for electrophilic and oxidative stress. We previously showed that vimentin interacts with zinc, which affects its assembly and redox sensing. Here we have used vimentin wt and C328S, an oxidation-resistant mutant showing improved NaCl-induced polymerization, to assess the impact of zinc on soluble and polymerized vimentin by light scattering and electron microscopy. Zinc acts as a switch, reversibly inducing the formation of vimentin oligomeric species. High zinc concentrations elicit optically-detectable vimentin structures with a characteristic morphology depending on the support. These effects also occur in vimentin C328S, but are not mimicked by magnesium. Treatment of vimentin with micromolar zinc induces fibril-like particles that do not assemble into filaments, but form aggregates upon subsequent addition of NaCl. In contrast, when added to NaCl-polymerized vimentin, zinc increases the diameter or induces lateral association of vimentin wt filaments. Remarkably, these effects are absent or attenuated in vimentin C328S filaments. Therefore, the zinc-vimentin interaction depends on the chemical environment and on the assembly state of the protein, leading to atypical polymerization of soluble vimentin, likely through electrostatic interactions, or to broadening and lateral association of preformed filaments through mechanisms requiring the cysteine residue. Thus, impact of zinc on vimentin assembly and redox regulation is envisaged.
Direct Ink Writing Glass: A Preliminary Step for Optical Application
Authors: Nan Bo , Gołębiewski Przemysław , Buczyński Ryszard , Rosales F.J. Galindo , Ferreira José
Published: March 2020

In this paper, we present a preliminary study and conceptual idea concerning 3D printing water-sensitive glass by exemplifying with a borosilicate glass with high alkali and alkaline oxide contents using direct ink writing. The investigated material was prepared in the form of a glass frit, which was further ground in order to obtain a fine powder of desired particle size distribution. In a following step, inks were prepared by mixing the fine glass powder with Pluoronic F-127 hydrogel. The acquired pastes were rheologically characterized and printed using a Robocasting device. DSC experiments were performed for base materials and the obtained green bodies. After sintering, SEM and XRD analyses were carried out in order to examine microstructure and the eventual presence of crystalline phase inclusions. Results confirmed that obtained inks exhibit stable rheological properties despite the propensity of glass to undergo hydrolysis and could be adjusted to desirable values for 3D printing. No additional phase was observed, supporting the suitability of the designed technology for the production of water sensitive glass inks. SEM micrographs of the sintered samples revealed the presence of closed porosity, which may be the main reason of light scattering.
The Impact of Student-Curated Exhibitions about Socio-Scientific Issues on Students’ Perceptions regarding their Competences and the Science Classes
Authors: Reis Pedro , Tinoca Luís , Baptista Mónica , Linhares Elisabete
Published: March 2020

The IRRESISTIBLE Project (FP7, Grant 612367) had the aim of involving teachers, students and the public in the discussion on Responsible Research and Innovation (RRI), promoting both the construction of knowledge on cutting-edge (and controversial) research topics and the discussion about the criteria that these research/innovation processes should respect in order to be considered as responsible. These criteria also represent a strong contribution to a more sustainable future for all. This quantitative research evaluates the impact of IRRESISTIBLE’s student-curated exhibitions – about the RRI dimensions of cutting-edge research topics (socio-scientific issues) – on students’ perceptions regarding their scientific competences and the science classes. A pre and post-questionnaire was developed, validated and applied to students from 10 countries. The overall results of the statistical analysis indicate that students improved their perceptions regarding their competences for developing exhibitions in science classes as a way of creating awareness on topics relating science-technology-society. This activity reinforced students’ perceptions that in science classes they: a) discuss current issues and how they impact their lives; b) develop socially and relevant projects; and c) learn how to influence other citizens’ decisions about social issues related to science, technology and environment with the aim of assuring a more sustainable future.
Multi-Physical Design and Resonant Controller Based Trajectory Tracking of the Electromagnetically Driven Fast Tool Servo
Authors: Hussain Imran , Xia Wei , Zhao Dongpo , Huang Peng , Zhu Zhiwei
Published: March 2020

In this paper, a voice coil motor (VCM) actuated fast tool servo (FTS) system is developed for diamond turning. To guide motions of the VCM actuator, a crossed double parallelogram flexure mechanism is selected featuring totally symmetric structure with high lateral stiffness. To facilitate the determination of the multi-physical parameters, analytical models of both electromagnetic and mechanical systems are developed. The designed FTS with balanced stroke and natural frequency is then verified through the finite element analysis. Finally, the prototype of the VCM actuated FTS is fabricated and experimentally demonstrated to have a stroke of ±59.02 μm and a first natural frequency of 253 Hz. By constructing a closed-loop control using PID controller with the internal-model based resonant controller, the error for tracking a harmonic trajectory with ±10 μm amplitude and 120 Hz frequency is obtained to be ±0.2 μm, demonstrating the capability of the FTS for high accuracy trajectory tracking.
Comparative Plastomics of Ashwagandha (Withania, Solanaceae) and Identification of Mutational Hotspots for Barcoding Medicinal Plants
Authors: Mehmood Furrukh , . Abdullah , Ubaid Zartasha , Bao Yiming , Poczai Peter , Mirza Bushra
Published: March 2020

Within the family Solanaceae, Withania is a small genus belonging to the Solanoideae subfamily. Here, we report the de novo assembled, complete, plastomed genome sequences of W. coagulans, W. adpressa, and W. riebeckii. The length of these genomes ranged from 154,198 base pairs (bp) to 154,361 bp and contained a pair of inverted repeats (IRa and IRb) of 25,027--25,071 bp that were separated by a large single-copy (LSC) region of 85,675--85,760 bp and a small single-copy (SSC) region of 18,457--18,469 bp. We analyzed the structural organization, gene content and order, guanine-cytosine content, codon usage, RNA-editing sites, microsatellites, oligonucleotide and tandem repeats, and substitutions of Withania plastid genomes, which revealed close resemblance among the species. Both the substitution and insertion and deletion analyses confirmed that the IR region was significantly conserved compared with the LSC and SSC regions. Further comparative analysis among the Withania species highlighted 30 divergent hotspots that could potentially be used for molecular marker development, phylogenetic analysis, and species identification.
Two Achilles' Heels of the Ebolavirus Glycoprotein?
Authors: Li Wei
Published: March 2020

Ebolavirus has a membrane envelope decorated by trimers of a glycoprotein (GP), which is responsible for host cell attachment and membrane fusion. Therefore, GP is a primary target for antiviral drugs development. Here, this article reports the first, to my knowledge, set of structural analysis of all Ebolavirus GP structures as of March 10, 2020, and also a brief update of the structurally identified electrostatic features of the Ebolavirus GP structures in both apo (unliganded) state and also in bound states with a series of small compounds, including a variety of approved drugs. With this comprehensive set of structural analysis, this article puts forward a hypothesis of two Achilles' heels of Ebolavirus GP structure, where the formation of two interfacial salt bridges, instead of destabilizing the prefusion conformation of Ebolavirus GP, constitutes a positive contribution towards the structural rigidification of the prefusion conformation of the Ebolavirus GP structure, thereby acting against GP-mediated Ebolavirus cell entry and/or preventing fusion between the viral and endosome membranes.
Purinergic Signaling in Neutrophils During Inflammatory Diseases
Authors: Sun Hui , Xu Gang , Li Pingsong , Li Yumei , Sun Bingwei
Published: March 2020

Purinergic signaling is that nucleotides (especially ATP) and adenosine are utilized as transmitter molecules, which play an important role in the immune system. In the extracellular ventricle, ATP plays a significant role of pro-inflammatory molecules mainly through activating P2 receptors, while adenosine plays the role of anti-inflammatory molecules mainly through activating P1 receptors. As we know，neutrophils are the most abundant immune cells in our circulation and have become an essential part of coordinating a series of complex events during inflammatory diseases. However, due to the destruction of inflammatory substances from neutrophils, the activation of neutrophils is fine-tuned, and purinergic signaling is associated with this process. As a matter of fact, altering the balance between P2 and P1 signals is of great importance for neutrophils to exert immune activities properly. Here, we review the role of purinergic signaling in regulatory function of neutrophils during inflammatory disease, and then discuss the potential contribution of targeted purinergic signals in the treatment of the neutrophil during inflammatory diseases.
Machine Learning Models for Predicting Hearing Prognosis in Unilateral Idiopathic Sudden Sensorineural Hearing Loss
Authors: Park Keon Vin , Oh Kyoung Ho , Jeong Yong Jun , Rhee Jihye , Han Mun Soo , Han Sung Won , Choi June
Published: March 2020

Objectives. Prognosticating idiopathic sudden sensorineural hearing loss (ISSNHL) is an important challenge. In our study, a dataset was split into training and test sets and cross-validation was implemented on the training set, thereby determining the hyperparameters for machine learning models with high test accuracy and low bias. The effectiveness of the following five machine learning models for predicting the hearing prognosis in patients with ISSNHL after 1 month of treatment was assessed: adaptive boosting, K-nearest neighbor, multilayer perceptron, random forest (RF), and support vector machine (SVM).Methods. The medical records of 523 patients with ISSNHL admitted to Korea University Ansan Hospital between January 2010 and October 2017 were retrospectively reviewed. In this study, we analyzed data from 227 patients (recovery, 106; no recovery, 121) after excluding those with missing data. To determine risk factors, statistical hypothesis tests (e.g., the two-sample t-test for continuous variables and the chi-square test for categorical variables) were conducted to compare patients who did or did not recover. Variables were selected using an RF model depending on two criteria (mean decreases in the Gini index and accuracy).Results. The SVM model using selected predictors achieved both the highest accuracy (75.36%) and the highest F-score (0.74) on the test set. The RF model with selected variables demonstrated the second-highest accuracy (73.91%) and F-score (0.74). The RF model with the original variables showed the same accuracy (73.91%) as that of the RF model with selected variables, but a lower F-score (0.73). All the tested models, except RF, demonstrated better performance after variable selection based on RF.Conclusion. The SVM model with selected predictors was the best-performing of the tested prediction models. The RF model with selected predictors was the second-best model. Therefore, machine learning models can be used to predict hearing recovery in patients with ISSNHL.
Macular hole edge morphology predicts restoration of postoperative retinal microstructure and functional outcome
Authors: Tao Jiwei , Chen Huan , Zhu Lin , Pan Deming , Fang Jia , Chen Yiqi , Mao Jianbo , Shen Lijun
Published: March 2020

Background: To investigate the association between iOCT feature in macular hole (MH) surgery and the restoration of the retina microstructure and visual outcomes. Methods: This was a case series study including fifty-three eyes of 53 patients with macular hole were recruited. According to the morphological characteristics of the hole edge as imaged by iOCT after ILM peeling, all patients were divided into three groups: fovel flap group, hole-door group, and negative group. The restoration of the retina microstructure and postoperative visual outcomes of the MH surgery were compared between these groups. Results: All of the eyes had MH closure after vitrectomy. The postoperative BCVA was significantly improved compared with the preoperative BCVA (P<0.001). Based on the features of the hole edge as reveal by iOCT, the negative group included 24 eyes, the fovea flap group included 14 eyes, and the hole-door group included 15 eyes. The hole-door group and foveal flap group had significantly better final visual acuity and postoperative restoration of the ELM than the negative group (P=0.002, P=0.012). For the group in which the MHD was ≤400 μm, there were no significant differences in ELM restoration, EZ restoration, or BCVA among the three groups (P=0.516 and P=0.179 respectively). For the MHD >400-μm group, the hole-door group and fovea flap group had significantly better final visual acuity and restoration of ELM than the negative group (P=0.013, P=0.005). Conclusion: This study describes a novel intraoperative sign using iOCT. This sign can provide useful predictive information for postoperative restoration of the retinal microstructure and visual outcomes of MHs, especially large ones
Awareness of Osteoporosis among 368 Residents in China: A Cross-Sectional Study
Authors: Oumer Kemal Sherefa , Liu Yawen , Yu Qiong , Wu Fan , Yang Shuman
Published: March 2020

Abstract
Background: Studies on osteoporosis awareness among the general population in China are still limited. We examined the level of osteoporosis awareness among residents in China, determined the risk factors associated with a lower level of osteoporosis awareness, and assessed the sources of their knowledge about osteoporosis.
Methods: We conducted a cross-sectional study among 368 general residents aged 30 years or older from 19 provinces during January-March 2018 in China. All participants were identified and interviewed face-to-face by medical students in Jilin University using a structured questionnaire. Osteoporosis awareness scores (percent of the correct answer) was determined across several domains, including definition, diagnosis, risk factors, and prevention of osteoporosis. We used multiple linear regression models to test the relationship between risk factors and overall awareness scores.
Results: The mean age of included participants was 52.9 ± 10.2 years, and 53% of them were male. Osteoporosis awareness score for definition was 77.7%, diagnosis 49.6%, risk factors 49.2%, treatment 60.5%, and prevention 69.9%. The overall awareness score was 67.8%. Lower family income and education level were significantly associated with lower overall awareness score (all p <0.05). Television or radio health program was reported to be their main source of knowledge about osteoporosis.
Conclusion: The awareness level for osteoporosis in our study is moderate; lower family income and education level were risk factors for lower awareness. Television or radio health programs had the greatest contribution to osteoporosis awareness.
Keywords: Osteoporosis, Awareness, Knowledge, China
Genetic and phenotypic associations between root architecture, arbuscular mycorrhizal fungi colonisation and low phosphate tolerance instrawberry (Fragaria × ananassa)
Authors: Cockerton Helen Maria , Li Bo , Stavridou Eleftheria , Johnson Abigail , Karlström Amanda , Armitage Andrew Douglas , Martinez-Crucis Ana , Arjona Lorena Galiano , Harrison Nicola , Barber-Pérez Nuria , Cobo-Medina Magdalena , Harrison Richard Jonathan
Published: March 2020

Background: Phosphate is an essential plant macronutrient required to achieve maximum crop yield. Roots are able to uptake soil phosphate from the immediate root area, thus creating a nutrient depletion zone. Many plants are able to exploit phosphate from beyond this root nutrient depletion zone through symbiotic association with Arbuscular Mycorrhizal Fungi (AMF). Here we characterise the relationship between root architecture, AMF association and low phosphate tolerance in strawberries. The contrasting root architecture in the parental strawberry cultivars ‘Redgauntlet’ and ‘Hapil’ was studied through a mapping population of 168 progeny. Low phosphate tolerance and AMF association was quantified for each genotype to allow assessment of the phenotypic and genotypic relationships between traits. Results: A “phosphate scavenging” root phenotype where individuals exhibit a high proportion of surface lateral roots was associated with a reduction in root system size across genotypes. A genetic correlation between “root system size” traits was observed with a network of pleiotropic QTL were found to represent five “root system size” traits. By contrast, average root diameter and the distribution of roots appeared to be under two discrete methods of genetic control. A total of 18 QTL were associated with plant traits, 4 of which were associated with solidity that explained 46 % of the observed variation. Investigations into the relationship between AMF association and root architecture found that a higher root density was associated with greater AMF colonisation across genotypes. However, no phenotypic correlation or genotypic association was found between low phosphate tolerance and the propensity for AMF association, nor root architectural traits when plants are grown under optimal nutrient conditions.Conclusions: Understanding the genetic relationships underpinning phosphate capture can inform the breeding of strawberry varieties with better nutrient use efficiency. Solid root systems were associated with greater AMF colonisation. However, low P-tolerance was not phenotypically or genotypically associated with root architecture traits in strawberry plants. Furthermore, a trade-off was observed between root system size and root architecture type, highlighting the energetic costs associated with a “phosphate scavenging” root architecture.
Unstable intertrochanteric fractures are associated with a greater hemoglobin drop during the perioperative period: a retrospective case control study
Authors: Lin Po-Hsun , Chien Jui-Teng , Hung Jung-Pin , Hong Chih-Kai , Tsai Tzung-Yi , Yang Chang-Chen
Published: March 2020

Background

With an increase in the elderly population, the occurrence of hip fractures, femoral neck fractures, and intertrochanteric fractures (ITFs) is also increasing. It is important to establish effective perioperative methods that would help reduce the morbidity and mortality rates associated with ITFs. The purpose of this study was to determine the effects of ITFs according to the AO classification for perioperative hemoglobin drop.

Methods

Seventy-six patients with ITFs classified as AO 31-A1 or A2 and fixated with intramedullary nails participated in this retrospective cohort study. Medical records of these patients were retrospectively reviewed from September 2016 to August 2018. The perioperative hemoglobin drop was chosen as the main outcome measure and calculated as the difference between pre- and postoperative hemoglobin levels. Multivariate linear regression analysis was performed and included the following variables: AO classification (A1.1-A2.1 [stable] vs. A2.2-A2.3 [unstable]), time interval between injury and surgery, age, body mass index, and the use of anticoagulants.

Results

Among the 76 patients who met the inclusion criteria, a significantly higher hemoglobin drop was observed in the AO 31 A2.2-A2.3 (unstable) group than in the AO 31 A1.1-A2.1 (stable) group (p = 0.04). The multivariate analysis also showed a greater hemoglobin drop in the unstable group (p < 0.05).

Conclusions

Patients with unstable ITFs exhibited a greater hemoglobin drop and a hidden blood loss was suspected around the fracture site. We believe that this should be taken into consideration when presurgical blood transfusion is being planned for patients with unstable ITFs, to reduce associated postoperative complications, especially in patients with severe anemia or high risk of mortality.
Bis-benzylidine Piperidone RA190 Treatment of Hepatocellular Carcinoma via Binding RPN13 and Inhibiting NF-κB Signaling
Authors: Soong Ruey-Shyang , Ravi Anchoori , Roden Richard , Cho Rou-Ling , Chen Yi-Chan , Tseng Sheng-Chieh , Huang Yun-Li , Liao Po-Cheng , Shyu Yu-Chiau
Published: March 2020

Background Hepatocellular carcinoma (HCC) is the fifth most common malignancy and the third leading cause of cancer mortality worldwide. The development of new anticancer agents targeting different pathways is imperative to improve the advanced HCC. The aberrant metabolism and aggressive growth of cancer cells can render them particularly susceptible to proteasome inhibition, as first demonstrated by the success of bortezomib treatment for multiple myeloma. However, resistance does emerge and this 20S proteasome inhibitor has not proven active against HCC. The bis-benzylidine piperidone RA190 represents a novel class of proteasome inhibitor that covalently binds to cysteine 88 of RPN13, a ubiquitin receptor subunit of the proteasome’s 19S regulatory particle. RA190 treatment inhibits proteasome function, causing rapid accumulation of polyubiquitinated proteins. Methods Human HCC cell lines were treated by RA190 in vitro in different concentration and time frame. We checked the killing effect and the possible mechanisms that lead the tumor apoptosis. We also performed the orthotopic HCC animal model to show the RA190 had significant killing effect in vivo . Results We showed RA190 is also toxic to HCC cells by triggering the rapid build-up of polyubiquitinated proteins, resulting in endoplasmic reticulum stress and the induction of cell death via apoptosis. Considerable evidence suggests that nuclear factor κB (NF-κB) signal is essential for promoting inflammation-associated cancer. Here, we showed that RA190 inhibited the NF-κB pathway in HCC by preventing the degradation of IκBα via the proteasome. Treatment of mice bearing an orthotopic HCC model with RA190 significantly reduced tumor growth. We therefore explored combining RA190 with a tyrosine kinase inhibitor currently used to the treat HCC, Sorafenib. Conclusions RA190 and Sorafenib exhibited synergetic killing of HCC cells in vitro , suggesting further exploration of such a combination treatment of HCC is warranted.
Factors influencing the perceived importance of oral health within a rural Aboriginal and Torres Strait Islander Community in Australia.
Authors: Tynan Anna , Walker David , Tucker Taygan , Fisher Barry , Fisher Tarita
Published: March 2020

Background
Indigenous Australians suffer from higher rates of oral disease and have more untreated dental problems and tooth extractions than the general population. Indigenous Australians also have lower rates of accessing oral health services and are more likely to visit for a problem rather than a check-up. Multiple issues effect health service and prevention programs including: characteristics of health services such as distances to health services; existence of social and cultural barriers; available wealth and social support; and, characteristics of the individual and community including the importance given to the disease. This paper seeks to explore the perceived importance of oral health within a rural Indigenous community in Australia and the factors influencing this perception.

Methods
The study used a phenomenology research design incorporating focus group discussions and in-depth interviews. It was undertaken in partnership with communities’ Health Action Group who guided the focus, implementation and reporting of the research. A convenience sample was recruited from established community groups. Thematic analysis on the transcripts was completed.

Results
Twenty- seven community members participated in three focus groups and twelve in-depth interviews. The study found that the community gives high priority to oral health. Factors influencing the importance include: the perceived severity of symptoms of oral disease such as pain experienced due to tooth ache; lack of enabling resources such as access to finance and transport; the social impact of oral disease on individuals including impact on their personal appearance and self-esteem; and health beliefs including oral health awareness. Participants also noted that the importance given to oral health within the community competed with the occurrence of multiple health concerns and family responsibilities.

Conclusion
This paper highlights the high importance this rural Indigenous community gives to oral health. Its findings suggest that under-utilisation of oral health services is influenced by both major barriers faced in accessing oral health services; and the number and severity of competing health and social concerns within the community. The study results confirm the importance of establishing affordable, culturally appropriate, community-based oral health care services to improve the oral health of rural Indigenous communities.
Habitat suitability modelling to assess the introductions of Aedes albopictus (Diptera, Culicidae) in the Netherlands
Authors: Ibañez-Justicia Adolfo , Alcaraz-Hernández Juan Diego , Lammeren Ron van , Koenraadt Constantianus J.M. , Bergsma Aldo , Delucchi Luca , Rizzoli Annapaola , Takken Willem
Published: March 2020

Background In the Netherlands, Aedes albopictus has been found each year since 2010 during routine exotic mosquito species surveillance at companies that import used tires. We developed habitat suitability models to investigate the potential risk of establishment and spread of this invasive species at these locations. Methods We used two methodologies: first, a species distribution model based on the maximum entropy modelling approach (MaxEnt) taking into consideration updated occurrence data of the species in Europe, and second, a spatial logic conditional model based on the temperature requirements of the species and using land surface temperature data (LST model). Results Suitability assessment obtained with the MaxEnt model at European level accurately reflect the current distribution of the species and these results also depict moderately low values in parts of the Netherlands, Belgium, Denmark, the British islands and southern parts of Scandinavia. Winter temperature was the variable that contributed most to the performance of the model (47.3%). The results of the LST model show that 1) coastal areas are suitable for overwintering of eggs, 2) large areas in the northern part of the country have a low suitability for adult survival, and 3) the entire country is suitable for successful completion of the life cycle if the species is introduced after the winter months. Results of the LST model reveal that temperatures in 2012 and 2014 did not limit the overwintering of eggs or survival of adults at the locations where the species was found. By contrast, for the years 2010, 2011 and 2013, overwintering of eggs at these locations is considered unlikely. Conclusions Results using two modelling methodologies show differences in predicted habitat suitability values. Based on the results of both models, the climatic conditions could hamper the successful overwintering of eggs of Ae. albopictus and their survival as adults in many areas of the country. However, during warm years with mild winters, many areas of the Netherlands offer climatic conditions suitable for developing populations. Regular updates of the models, using updated occurrence and climatic data, are recommended to study the areas at risk.
Minocycline prevents and repairs the skin disorder associated with afatinib, one of the epidermal growth factor receptor-tyrosine kinase inhibitors for non-small cell lung cancer
Authors: Sano Kazumi , Nakadate Kazuhiko , Hanada Kazuhiko
Published: March 2020

Background While epidermal growth factor receptor(EGFR) tyrosine kinase inhibitors(TKIs) exert a breakthrough effect, the incidence of skin disorders as a side effect has significantly reduced patients' quality of life. Objective This study aimed to develop a treatment for inflammatory ulcers as one of the side effects of afatinib (Geotrif®), a second-generation EGFR-TKI, and established a skin disorder mouse model to investigate the protective effect of minocycline. Methods First, under inhalation anesthesia with isoflurane, the back of a male ddy mouse was shaved, and afatinib petrolatum was applied alone or in combination with minocycline to observe the state of the skin and measure transepidermal water transpiration (TEWL). Next, afatinib was administered orally to mice, and minocycline petrolatum was applied to observe whether the skin disorder was prevented and its effect on repair of the skin disorder. Results Skin injury occurred on the back of the mouse following afatinib (1 mg/g in petrolatum) application, and scab formation was observed. Application of minocycline prevented and improved the skin disorder caused by afatinib. When the minocycline-petrolatum mixture was applied to the mouse that developed the skin disorder, a significant improvement in TEWL was observed, and skin repair was observed macroscopically. Discussion These results suggest that minocycline applied both locally and orally prevents and repairs afatinib-induced skin disorders. Histological examination of skin elucidated the mechanism of the occurrence of the EGFR-TKI-related skin disorder and suggested the efficacy of minocycline topical application in clinical practice.
Estimating runtime of a job in Hadoop MapReduce
Authors: Peyravi Narges , Moeini Ali
Published: March 2020

Hadoop MapReduce is a framework to process vast amounts of data in the cluster of machines in a reliable and fault-tolerant manner. Since being aware of the runtime of a job is crucial to subsequent decisions of this platform and being better management, in this paper we propose a new method to estimate the runtime of a job. For this purpose, after analysis the anatomy of processing a job in Hadoop MapReduce precisely, we consider two cases: when a job runs for the first time or a job has run previously. In the first case, by considering essential and efficient parameters that higher impact on runtime we formulate each phase of the Hadoop execution pipeline and state them by mathematical expressions to calculate runtime of a job. In the second case, by referring to the profile or history of a job in the database and use a weighting system the runtime is estimated. The results show the average error rate is less than 12% in the estimation of runtime for the first run and less than 8.5% when the profile or history of the job has existed.
Cavernous malformation hemorrhage due to trans-mural pressure alterations after cerebrospinal fluid diversion: a case report
Authors: Hartley Benjamin , Birnbaum Corinne , Hoffman Caitlin E
Published: March 2020

Background: Cavernous malformations are rare cerebral pseudo-vascular lesions with annualized bleeding rates of 0.5 – 3% in most studies. Of the various explored risk factors for bleeding to date, only prior hemorrhage has shown significant correlation. Case Presentation: In this case, we describe a 65-year old man with a peri-ventricular atrial cavernous malformation that hemorrhaged after CSF diversion via ventriculoperitoneal shunting. Serial imaging showed that bleeding continued until the shunt was revised with a programmable valve set at maximum resistance with the addition of a gravitational unit, thereby lowering the trans-mural pressure differential across the cavernous malformation. Conclusions: Given that other vascular lesions are subject to hemorrhage from alterations in trans-mural pressure dynamics, we hypothesize that cavernous malformations are similarly affected by trans-mural pressure gradients as they are composed of primitive vascular elements. This hypothesis is corroborated by the temporal correlation of interventions, imaging, and exam findings in the present case, and suggests a potentially important risk factor for hemorrhage in CM patients that affects prognostication and management.
microRNA profiling in the Weddell Seal suggests novel regulatory mechanisms contributing to diving adaptation
Authors: Dolfin Luca Penso , Haerty Wilfried , Hindle Allyson , Palma Federica Di
Published: March 2020

Background. The Weddell Seal (Leptonychotes weddelli) represents a remarkable example of adaptation to diving among marine mammals. This species is capable of diving >900 m deep and remaining underwater for more than 60 minutes. A number of key physiological specializations have been identified, including the low levels of aerobic, lipid-based metabolism under hypoxia, significant increase in oxygen storage in blood and muscle; high blood volume and extreme cardiovascular control. These adaptations have been linked to increased abundance of key proteins, suggesting an important, yet still understudied role for gene reprogramming. In this study, we investigate the possibility that post-transcriptional gene regulation by microRNAs (miRNAs) has contributed to the adaptive evolution of diving capacities in the Weddell Seal. Results. Using small RNA data across 4 tissues (cortex, heart, muscle and plasma), in 3 biological replicates, we generate the first miRNA annotation in this species, consisting of 559 high confidence, manually curated miRNA loci. Evolutionary analyses of miRNA gain and loss highlight a high number of Weddell seal specific miRNAs. 416 miRNAs were differentially expressed (DE) among tissues, whereas 83 miRNAs were differentially expressed (DE) across all tissues between pups and adults and 188 miRNAs demonstrated developmental changes in specific tissues only. mRNA targets of these altered miRNAs identify possible protective mechanisms in individual tissues, particularly relevant to hypoxia tolerance, anti-apoptotic pathways, and nitric oxide signal transduction. Novel, lineage-specific miRNAs associated with developmental changes target genes with roles in angiogenesis and vasoregulatory signaling. Conclusions. Altogether, we provide an overview of miRNA composition and evolution in the Weddell seal, and the first insights into their possible role in the specialization to diving.