bluuebunny/crossref_metadata_2025 huggingface dataset:
Two new coastal time-series of seawater carbonate system variables in the NW Mediterranean Sea: rates and mechanisms controlling pH changes
Authors: García-Ibáñez Maribel I. , Guallart Elisa F. , Lucas Arturo , Pascual Josep , Gasol Josep M. , Marrasé Cèlia , Calvo Eva , Pelejero Carles
Published: February 2024

In this work, we present, for the first time, the seawater carbonate system measurements of two coastal time-series in the NW Mediterranean Sea, L’Estartit Oceanographic Station (EOS; 42.05°N 3.2542°E) and the Blanes Bay Microbial Observatory (BBMO; 41.665°N 2.805°E). At these two time-series, measurements of total alkalinity (TA), pH, and associated variables, such as dissolved inorganic nutrients, temperature, and salinity, have been performed monthly since 2010 in surface seawater. Seasonality and seasonal amplitude are analogous in both time-series, with seasonality in pHTin situ(pH at in situ seawater conditions on the total hydrogen ion scale) primarily determined by seasonality in sea surface temperature. The evaluated pHTin situtrends at BBMO (-0.0021 ± 0.0003 yr-1) and EOS (-0.0028 ± 0.0005 yr-1) agree with those reported for coastal and open ocean surface waters in the Mediterranean Sea and open ocean surface waters of the global ocean, therefore indicating that these time-series are representative of global ocean acidification signals despite being coastal. The decreases in pHTin situcan be attributed to increases in total dissolved inorganic carbon (DIC; 1.5 ± 0.4 µmol kg-1 yr-1 at BBMO and 1.6 ± 0.6 µmolESkg-1 yr-1 at EOS) and sea surface temperature (0.08 ± 0.02 °C yr-1 at BBMO and 0.08 ± 0.04 °C yr-1 at EOS). The increases in carbon dioxide fugacity (fCO2; 2.4 ± 0.3 µmol kg-1 yr-1 at BBMO and 2.9 ± 0.6 µmol kg-1 yr-1 at EOS) follow the atmospheric CO2 forcing, thus indicating the observed DIC increase is related to anthropogenic CO2 uptake. The increasing trends in TA (1.2 ± 0.3 µmol kg-1 yr-1 at BBMO and 1.0 ± 0.5 µmol kg-1 yr-1 at EOS) buffered the acidification rates, counteracting 60% and 72% of the pHTin situdecrease caused by increasing DIC at EOS and BBMO, respectively. Once accounted for the neutralizing effect of TA increase, the rapid sea surface warming plays a larger role in the observed pH decreases (43% at EOS and 62% at BBMO) than the DIC increase (36% at EOS and 33% at BBMO).
Dynamics of antiphase bursting modulated by the inhibitory synaptic and hyperpolarization-activated cation currents
Authors: Guan Linan , Gu Huaguang , Zhang Xinjing
Published: February 2024

Antiphase bursting related to the rhythmic motor behavior exhibits complex dynamics modulated by the inhibitory synaptic current (Isyn), especially in the presence of the hyperpolarization-activated cation current (Ih). In the present paper, the dynamics of antiphase bursting modulated by the Ih and Isyn is studied in three aspects with a theoretical model. Firstly, the Isyn and the slow Ih with strong strength are the identified to be the necessary conditions for the antiphase bursting. The dependence of the antiphase bursting on the two currents is different for low (escape mode) and high (release mode) threshold voltages (Vth) of the inhibitory synapse. Secondly, more detailed co-regulations of the two currents to induce opposite changes of the bursting period are obtained. For the escape mode, increase of the Ih induces elevated membrane potential of the silence inhibited by a strong Isyn and shortened silence duration to go beyond Vth, resulting in reduced bursting period. For the release mode, increase of the Ih induces elevated tough value of the former part of the burst modulated by a nearly zero Isyn and lengthen burst duration to fall below Vth, resulting in prolonged bursting period. Finally, the fast-slow dynamics of the antiphase bursting are acquired. Using one-and two-parameter bifurcations of the fast subsystem of a single neuron, the burst of the antiphase bursting is related to the stable limit cycle, and the silence modulated by a strong Isyn to the stable equilibrium to a certain extent. The Ih mainly modulates the dynamics within the burst and quiescent state. Furthermore, with the fast subsystem of the coupled neurons, the silence is associated with the unstable equilibrium point. The results present theoretical explanations to the changes in the bursting period and fast-slow dynamics of the antiphase bursting modulated by the Isyn and Ih, which is helpful for understanding the antiphase bursting and modulating rhythmic motor patterns.
COVID-19 booster enhances IgG mediated viral neutralization by human milk in vitro
Authors: Valcarce Vivian , Stafford Lauren Stewart , Neu Josef , Parker Leslie , Vicuna Valeria , Cross Tyler , D'Agati Olivia , Diakite Sisse , Haley Addison , Feigenbaum Jake , Al Mahmoud Mahmoud Y. , Visvalingam Anjali , Cacho Nicole , Kosik Ivan , Yewdell Jonathan W. , Larkin Joseph
Published: February 2024

Background

Facilitated by the inability to vaccinate, and an immature immune system, COVID-19 remains a leading cause of death among children. Vaccinated lactating mothers produce specific SARS-CoV-2 antibodies in their milk, capable of neutralizing the virus in vitro. Our objective for this study is to assess the effect of COVID-19 booster dose on SARS-CoV-2 antibody concentration and viral neutralization in milk, plasma, and infant stool.

Methods

Thirty-nine mothers and 25 infants were enrolled from December 2020 to May 2022. Milk, maternal plasma, and infants' stool were collected at various time-points up to 12 months following mRNA COVID-19 vaccination. A subgroup of 14 mothers received a booster dose. SARS-CoV-2 antibody levels and their neutralization capacities were assessed.

Results

Booster vaccination led to significantly higher IgG levels within human milk and breastfed infants' stool. In vitro neutralization of VSV-gfp-SARS-CoV-2-S-gp, a laboratory safe SARS-CoV-2 like pseudovirus, improved following the booster, with a 90% increase in plasma neutralization and a 60% increase in milk neutralization. We found that post-booster neutralization by human milk was highly correlated to SARS-CoV-2 IgG level. In support of our correlation result, Protein G column depletion of IgG in milk yielded a significant reduction in viral neutralization (p = 0.04).

Discussion

The substantial increase in neutralizing IgG levels in milk and breastfed infants' stool post-booster, coupled with the decrease in milk neutralization capabilities upon IgG depletion, underscores the efficacy of booster doses in augmenting the immune response against SARS-CoV-2 in human milk.
Advances in statistical methods for cancer surveillance research: an age-period-cohort perspective
Authors: Rosenberg Philip S. , Miranda-Filho Adalberto
Published: February 2024

Background

Analysis of Lexis diagrams (population-based cancer incidence and mortality rates indexed by age group and calendar period) requires specialized statistical methods. However, existing methods have limitations that can now be overcome using new approaches.

Methods

We assembled a “toolbox” of novel methods to identify trends and patterns by age group, calendar period, and birth cohort. We evaluated operating characteristics across 152 cancer incidence Lexis diagrams compiled from United States (US) Surveillance, Epidemiology and End Results Program data for 21 leading cancers in men and women in four race and ethnicity groups (the “cancer incidence panel”).

Results

Nonparametric singular values adaptive kernel filtration (SIFT) decreased the estimated root mean squared error by 90% across the cancer incidence panel. A novel method for semi-parametric age-period-cohort analysis (SAGE) provided optimally smoothed estimates of age-period-cohort (APC) estimable functions and stabilized estimates of lack-of-fit (LOF). SAGE identified statistically significant birth cohort effects across the entire cancer panel; LOF had little impact. As illustrated for colon cancer, newly developed methods for comparative age-period-cohort analysis can elucidate cancer heterogeneity that would otherwise be difficult or impossible to discern using standard methods.

Conclusions

Cancer surveillance researchers can now identify fine-scale temporal signals with unprecedented accuracy and elucidate cancer heterogeneity with unprecedented specificity. Birth cohort effects are ubiquitous modulators of cancer incidence in the US. The novel methods described here can advance cancer surveillance research.
Evaluation of clinical and imaging features for differentiating rhabdomyosarcoma from neuroblastoma in pediatric soft tissue
Authors: Sheng Jing , Li Tingting , Xu Huafeng , Xu Rong , Cai Xuemei , Zhang Huanhuan , Ji Qiongqiong , Duan Xiuhua , Xia Weiwei , Yang Xiujun
Published: February 2024

Background

In this study, we developed a nomogram predictive model based on clinical, CT, and MRI parameters to differentiate soft tissue rhabdomyosarcoma (RMS) from neuroblastoma (NB) in children preoperatively.

Materials and methods

A total of 103 children with RMS (n=37) and NB (n=66) were enrolled in the study from December 2012 to July 2023. The clinical and imaging data (assessed by two experienced radiologists) were analyzed using univariate analysis, and significant factors were further analyzed by multivariable logistic regression using the forward LR method to develop the clinical model, radiological model, and integrated nomogram model, respectively. The diagnostic performances, goodness of fit, and clinical utility of the integrated nomogram model were assessed using the area under the curve (AUC) of the receiver operator characteristics curve (ROC) with a 95% confidence interval (95% CI), calibration curve, and decision curve analysis (DCA) curves, respectively. Diagnostic efficacy between the model and radiologists’ interpretations was examined.

Results

The median age at diagnosis in the RMS group was significantly older than the NB group (36.0 months vs. 14.5 months; P=0.003); the fever rates in RMS patients were significantly lower than in patients with NB (0.0% vs.16.7%; P=0.022), and the incidence of palpable mass was higher in patients with RMS compared with the NB patients (89.2% vs. 34.8%; P<0.001). Compare NB on image features: RMS occurred more frequently in the head and neck and displayed homogeneous density on non-enhanced CT than NB (48.6% vs. 9.1%; 35.3% vs. 13.8%, respectively; all P<0.05), and the occurrence of characteristics such as calcification, encasing vessels, and intraspinal tumor extension was significantly less frequent in RMS children compared to children with NB (18.9% vs. 84.8%; 13.5% vs. 34.8%; 2.7% vs. 50.0%, respectively; all P <0.05). Two, three, and four features were identified as independent parameters by multivariate logistic regression analysis to develop the clinical, radiological, and integrated nomogram models, respectively. The AUC value (0.962), calibration curve, and DCA showed that the integrated nomogram model may provide better diagnostic performance, good agreement, and greater clinical net benefits than the clinical model, radiological model, and radiologists’ subjective diagnosis.

Conclusion

The clinical and imaging features-based nomogram has potential for helping radiologists distinguish between pediatric soft tissue RMS and NB patients preoperatively, and reduce unnecessary interventions.
Case report: Rare oral manifestations in Cowden syndrome with PTEN mutation
Authors: Yuan Wei , Liu Yanbin , Sun Haibin , Su Ming , Qin Lizheng , Huang Xin
Published: February 2024

Background

Cowden syndrome (CS) is a rare genetic disorder associated with PTEN gene mutations. It is characterized by macrocephaly, specific mucocutaneous features, and a predisposition to benign and malignant tumors. Cases of CS primarily presenting with oral clinical manifestations are relatively uncommon.

Methods/Results

We report the case of a 41-year-old male proband who presented with bilateral commissural and lingual externally projecting symmetric lesions for over two years. The proband also exhibited other features, including macrocephaly, communication difficulties, and obesity. Similar oral clinical manifestations were observed in family members. Whole exome sequencing analysis revealed PTEN gene mutations associated with CS in both the proband and his younger brother. This case serves as a reminder to be aware of the diverse presentations of CS in oral clinical practice and highlights the importance of genetic testing for guiding diagnosis and treatment.

Conclusion

There are few reported cases of CS primarily presenting with oral lesions. This finding contributes to further understanding of certain aspects of the pathogenesis of CS and enhances awareness of CS cases primarily exhibiting oral clinical manifestations.
Natural compounds target programmed cell death (PCD) signaling mechanism to treat ulcerative colitis: a review
Authors: Chen Bo , Dong Xinqian , Zhang Jin Long , Sun Xitong , Zhou Lin , Zhao Kangning , Deng Hualiang , Sun Zhen
Published: February 2024

Ulcerative colitis (UC) is a nonspecific inflammatory bowel disease characterized by abdominal pain, bloody diarrhea, weight loss, and colon shortening. However, UC is difficult to cure due to its high drug resistance rate and easy recurrence. Moreover, long-term inflammation and increased disease severity can lead to the development of colon cancer in some patients. Programmed cell death (PCD) is a gene-regulated cell death process that includes apoptosis, autophagy, necroptosis, ferroptosis, and pyroptosis. PCD plays a crucial role in maintaining body homeostasis and the development of organs and tissues. Abnormal PCD signaling is observed in the pathological process of UC, such as activating the apoptosis signaling pathway to promote the progression of UC. Targeting PCD may be a therapeutic strategy, and natural compounds have shown great potential in modulating key targets of PCD to treat UC. For instance, baicalin can regulate cell apoptosis to alleviate inflammatory infiltration and pathological damage. This review focuses on the specific expression of PCD and its interaction with multiple signaling pathways, such as NF-κB, Nrf2, MAPK, JAK/STAT, PI3K/AKT, NLRP3, GPX4, Bcl-2, etc., to elucidate the role of natural compounds in targeting PCD for the treatment of UC. This review used (ulcerative colitis) (programmed cell death) and (natural products) as keywords to search the related studies in PubMed and the Web of Science, and CNKI database of the past 10 years. This work retrieved 72 studies (65 from the past 5 years and 7 from the past 10 years), which aims to provide new treatment strategies for UC patients and serves as a foundation for the development of new drugs.
Metabolic dysfunction-associated steatotic liver disease increases hepatocellular carcinoma risk in chronic hepatitis B patients: a retrospective cohort study
Authors: Lin Ming , Gao Bowen , Peng Mengnan , Chen Xuefang , Xiao Huanming , Shi Meijie , Zhang Xiujuan , Zeng Folai , Chi Xiaoling
Published: February 2024

Background: The combined effect of hepatitis B virus infection and metabolic dysfunction-associated steatotic liver disease (MASLD) on hepatocellular carcinoma (HCC) risk remains unclear. The current study sought to elucidate the impact of MASLD on HCC progression in chronic hepatitis B (CHB) patients.

Method: This retrospective cohort study included CHB patients who had undergone liver biopsy and abdominal imaging at the Guangdong Provincial Hospital of Chinese Medicine between 2013 and 2019. We investigated the correlation between MASLD and HCC risk, and inverse probability treatment weighting (IPTW) was used to adjust for patient characteristics.

Results: A total of 1,613 patients were included, and 483 (29.9%) were diagnosed with MASLD. Over a median follow-up period of 5.02 years, 36 (2.2%) developed HCC, comprising 4.8% (23/483) of those with MASLD and 1.2% (13/1,130) of those without. Those with MASLD had a significantly higher cumulative incidence of HCC than those without (p < 0.001). The presence of MASLD was associated with a higher risk of HCC (adjusted hazard ratio [HR], 3.996; 95% confidence interval [CI], 2.007–7.959; p < 0.001). After adjustment using IPTW, the patients with MASLD retained a higher cumulative incidence of HCC (p < 0.001). Moreover, MASLD was found to be an independent risk factor for the development of HCC (adjusted HR, 10.191; 95% CI, 4.327–24.002; p < 0.001). However, among patients with MASLD, there were no significant differences in the cumulative risk of HCC between patients with and without overweight, between those with <2 and ≥2 cardiometabolic risk factors (CMRFs), between those with <3 and ≥3 CMRFs, or between those with <4 and ≥4 CMRFs (p = 0.110, p = 0.087, p = 0.066, and p = 0.490, respectively).

Conclusion: The presence of MASLD is associated with a higher risk of HCC in patients with CHB. Notably, this higher risk is present in patients with MASLD, irrespective of the presence or absence of overweight or the number of CMRFs they have.
Impact of self-control and time perception on intertemporal choices in gain and loss situations
Authors: Qu Weiguo , Yang Ying , Zhou Mengjie , Fan Wei
Published: February 2024

Individuals frequently encounter dilemmas in which they must choose between smaller, immediate gains and larger, delayed rewards; this phenomenon is known as intertemporal choice. The present study analyzed the interplay of trait and state self-control and time perception tendencies (time overestimation vs. time underestimation) and how it influences the rates of selecting immediate options in both gain and loss situations by conducting an intertemporal choice task. Experiment 1 was used to explore the impact of trait self-control and time perception on intertemporal choices within gain and loss situations. In Experiment 2, the e-crossing task was used to induce self-control resource depletion in participants and to investigate the impact of self-control resources and time perception on intertemporal choices in gain and loss situations. The results indicate that (1) compared with the high-self-control group, the low-self-control group exhibited a greater tendency to choose immediate options. Additionally, the high time estimation group was more likely to opt for immediate choices than the low time estimation group was. Furthermore, participants were more likely to select immediate options in the loss situation than in the gain situation. (2) In the gain situation, the high time estimation group was more likely to choose immediate options than was the low time estimation group. However, in the loss situation, the difference between the two groups was nonsignificant. (3) Time perception and gain–loss situations exerted a moderating mediating effect on the impact of self-control resources on intertemporal choices. These findings shed light on the influence of both self-control abilities and self-control resources on intertemporal choices. They provide valuable insights into intertemporal decision behaviors across diverse contexts and indicate the need for rational analysis based on one’s current state to mitigate cognitive biases to ensure individuals can maximize benefits in their daily lives.
Interoceptive experiences and ecological care: an embodied approach within therapeutical realms
Authors: González-Grandón Ximena , Cadena-Alvear Itzel , Gastelum-Vargas Melina
Published: February 2024

The conventional dichotomy between human health and disease has historically been approached through reductionist models that emphasize the exclusive causal relevance of physiological and pathological processes. Consequently, self-awareness and affective dimensions, integral to a phenomenological perspective, are often relegated to secondary traits, affording little consideration for the causal role of embodied living organization. Our interest lies in exploring the potential relevance of the phenomenology of embodied self-awareness in relation to interoceptive processes within therapeutic settings. As we illustrate, when the unfolding processes of interoceptive awareness and its affective capacity take precedence, the agent assumes an active, rather than passive, role in their own experience of health or illness. Departing from an enactive, phenomenological, and ecological standpoint, we propose a distinctive perspective on interoceptive processes, relying on an affective conceptualization of a spectrum of experiences of bodily being-in-the-world. Our primary argument posits that considering interoceptive processes from an embodied and ecological viewpoint of the self, interacting with the material and social environment, enables an approach to the gradient of affective experiences of embodied self-awareness—where pleasure or suffering is perceived and felt—in a naturalized, non-reductive, and relational manner. We discern two ways in which interoceptive processes interrelate with the experience of embodied self-awareness: sensitivity (self-affective) and affective-laden perception. Drawing on this distinction, we provide a nuanced description of these experiences within communities of cis-women, exemplified through the contexts of menstruation and endometriosis. This exploration seeks to enhance our understanding of the phenomenology of embodied, ecological, and affective self-experience from within diverse and situated bodies. The goal is to contribute to their autonomy and ability to adapt and self-regulate within therapeutic contexts.
The effect of age and resilience on the dose–response function between the number of adversity factors and subjective well-being
Authors: Hsieh Shulan , Chang Yun-Hsuan , Yao Zai-Fu , Yang Meng-Heng , Yang Cheng-Ta
Published: February 2024

Background

Encountering challenges and stress heightens the vulnerability to mental disorders and diminishes well-being. This study explores the impact of psychological resilience in the context of adverse events, considering age-related variations in its influence on well-being.

Methods

A total of 442 participants (male vs. female =48% vs. 52%) with a mean age of 41.79 ± 16.99 years were collected and completed the following questionnaires Brief Betrayal Trauma Survey (BBTS), Brief Resilience Scale (BRS), Peace of Mind (PoM), The World Health Organization Quality of Life-BREF (WHOQOL-BREF), and Social Support Questionnaire (SSQ). They all underwent structural and resting-state functional magnetic resonance imaging (MRI) scans.

Results

Participants were categorized based on adversity levels: 34.39% faced one, 26.24% none, and 19.91, 9.50, and 8.14% encountered two, three, and four adversities, respectively. This categorization helps assess the impact on participants’ experiences. As adversity factors increased, PoM decreased. Controlling for age improved PoM model fit (ΔR2 = 0.123, p < 0.001). Adversity factors and age explained 14.6% of PoM variance (df = 2, F = 37.638, p < 0.001). PoM decreased with more adversity and increased with higher age.

Conclusion

The study found most participants faced at least one adversity. Adversity negatively affected PoM scores, while resilience acted as a protective factor. Resilience plays a crucial role in buffering the impact of adversities on well-being. Among those with high adversity, higher resilience correlated with stronger DMN-right frontal pole connectivity. Brain volume showed no significant differences, but the quality of life and social support varied between subgroups, with no differences in personal demographic and biophysical features.
The authenticity of souvenirs: examples from Taiwan
Authors: Wall Geoffrey
Published: February 2024

The purpose of this study is to demonstrate that authenticity is a complicated and imprecise concept. Using autoethnography as an approach to the study and, in particular, a consideration of the meanings of aboriginal souvenirs originating in Taiwan that are in the authors' possession, it is shown that these objects do not fit snuggly into typical classifications that have been proposed to address authenticity. Authenticity is a concept that can hide issues of power and powerlessness. It is suggested that authentication, who says something is authentic and why they do so, is important as it facilitates consideration of access to power.
Table tennis players use superior saccadic eye movements to track moving visual targets
Authors: Nakazato Riku , Aoyama Chisa , Komiyama Takaaki , Himo Ryoto , Shimegi Satoshi
Published: February 2024

Introduction

Table tennis players perform visually guided visuomotor responses countlessly. The exposure of the visual system to frequent and long-term motion stimulation has been known to improve perceptual motion detection and discrimination abilities as a learning effect specific to that stimulus, so may also improve visuo-oculomotor performance. We hypothesized and verified that table tennis players have good spatial accuracy of saccades to moving targets.

Methods

University table tennis players (TT group) and control participants with no striking-sports experience (Control group) wore a virtual reality headset and performed two ball-tracking tasks to track moving and stationary targets in virtual reality. The ball moved from a predetermined position on the opponent's court toward the participant's court. A total of 54 conditions were examined for the moving targets in combinations of three ball trajectories (familiar parabolic, unfamiliar descent, and unfamiliar horizontal), three courses (left, right, and center), and six speeds.

Results and discussion

All participants primarily used catch-up saccades to track the moving ball. The TT group had lower mean and inter-trial variability in saccade endpoint error compared to the Control group, showing higher spatial accuracy and precision, respectively. It suggests their improvement of the ability to analyze the direction and speed of the ball's movement and predict its trajectory and future destination. The superiority of the spatial accuracy in the TT group was seen in both the right and the left courses for all trajectories but that of precision was for familiar parabolic only. The trajectory dependence of improved saccade precision in the TT group implies the possibility that the motion vision system is trained by the visual stimuli frequently encountered in table tennis. There was no difference between the two groups in the onset time or spatial accuracy of saccades for stationary targets appearing at various positions on the ping-pong table.

Conclusion

Table tennis players can obtain high performance (spatial accuracy and precision) of saccades to track moving targets as a result of motion vision ability improved through a vast amount of visual and visuo-ocular experience in their play.
Exploring actual and perceived levels of physical activity intensity during virtual reality active games
Authors: Naugle Keith E. , Cervantes Xzaliya A. , Boone Carolyn L. , Wind Brandon , Naugle Kelly M.
Published: February 2024

Background

Research suggests that engaging in active virtual reality (VR) video games can elicit light to moderate levels of physical activity (PA), making it a novel and fun mode of exercise. Further research is needed to understand the influence of VR on perceptions of exertion and enjoyment during PA.

Objective

The objectives of this study are (1) to compare actual and perceived exertion within and between active VR games with varying levels of difficulty and (2) to determine how playing active VR games influences PA enjoyment during gameplay.

Methods

A total of 18 participants completed four separate study sessions, during which they engaged in either a 15-min bout of traditional exercise (stationary cycling) or played one VR game. Heart rate (HR) and ratings of perceived exertion (RPE) using the Borg CR10 scale were assessed during VR gameplay and cycling. Enjoyment was measured after gameplay. VR games included playing Holopoint at level 2 and level 3 and Hot Squat. Repeated measures ANOVAs were used to examine (1) changes in HR and RPE across time within games and (2) differences in actual and perceived levels of intensity and enjoyment between games. Bivariate correlations examined the relationship between the degree of change in actual intensity and the degree of change in perceived intensity during each VR game and cycling.

Results

The analyses revealed that RPE and HR significantly increased from baseline during each condition and generally increased across the 15-min of gameplay. Hot Squat and cycling elicited a significantly higher percentage of heart rate reserve (%HRR) than Holopoint at levels 2 and 3. Holopoint level 3 elicited a higher %HRR than Holopoint level 2. The participants reported greater average and max RPE during Hot Squat and cycling compared with Holopoint at levels 2 and 3. The correlations revealed a significant positive correlation between the degree of change in HR and RPE for cycling, but no significant correlations were observed for any of the VR conditions. The physical activity during Holopoint at both levels was rated as more enjoyable than Hot Squat and cycling.

Conclusion

Our data support the notion that VR has the potential to alter individuals’ perceptions of exertion during PA and, in particular, may reduce their awareness of increases in actual exertion.
A Review of Mechanisms to Vary the Stiffness of Laminar Jamming Structures and Their Applications in Robotics
Authors: Caro Freddy , Carmichael Marc G.
Published: February 2024

Laminar jamming (LJ) is a method to achieve variable stiffness in robotics that has attracted notable attention because of its simple working principle and potential high stiffness variation. This article reviews the lock/unlock mechanisms of LJ structures. The application of these mechanisms in robotics is discussed, including grippers, continuum robots, wearable robots, robot arms, and more. Furthermore, the performance and limitations of the mechanisms to vary the stiffness of LJ are qualitatively and quantitatively analyzed. This performance analysis focuses mainly on the potential of LJ mechanisms to be applied in robot arms with variable stiffness and their potential to attenuate the impact between human beings and robot arms. The modeling of LJ through analytical and finite element methods is described, and their evolution towards design methodologies is discussed. To conclude, the directions and recommendations that should be followed in research on LJ are discussed. These include the improvement of existing lock/unlock mechanisms, the development of new lock/unlock mechanisms, and the development of more control algorithms for robot arms that incorporate LJ structures.
Multi-Span Greenhouse Energy Saving by External Insulation: System Design and Implementation
Authors: Guan Wenfei , Guo Wenzhong , Chen Fan , Han Xiaobei , Wang Haiguang , Sun Weituo , Zhao Qian , Jia Dongdong , Wei Xiaoming , Zhu Qingzhen
Published: February 2024

To address the issues of excessive heat loss from the roofs of multi-span greenhouses and high energy consumption for heating during winter production, we propose an approach for the external insulation of the roof of multi-span glass greenhouses and have developed an external insulation system (EIS) to practice this approach. The system achieved full coverage of the greenhouse roof through mechanized unfurling and furling of external thermal blankets, thereby achieving energy-saving insulation. This paper describes the overall design and working method of the EIS, providing detailed design and structural parameters for critical components such as the traction rope transmission mechanism and the rail-type sealing structure. Through a system verification experiment, the specifications of the traction rope were determined and the rationality of the EIS’s thermal blanket unfurling and furling time was confirmed. An insulation performance experiment indicated that the average heat flux of the greenhouse roof covered with the external thermal blanket over 14 continuous nights was 54.2 W/m2, compared with 198.6 W/m2 for a single-layer glass roof. Covering the roof with the external thermal blanket reduced heat loss from the glass roof by 72.7%. The average heat flux of the roof of the Venlo-type multi-span greenhouse with double-layer internal insulation was 99.9 W/m2 during the same period, indicating that the heat loss from the roof using external insulation was only 50.3%. This study provides a novel thermal insulation approach and an energy-saving system for multi-span greenhouses.
When an Intruder Comes Home: GM and GE Strategies to Combat Virus Infection in Plants
Authors: Rahman Adeeb , Sanan-Mishra Neeti
Published: February 2024

Viruses are silent enemies that intrude and take control of the plant cell’s machinery for their own multiplication. Infection by viruses and the resulting damage is still a major challenge in the agriculture sector. Plants have the capability to fight back, but the ability of viruses to mutate at a fast rate helps them to evade the host’s response. Therefore, classical approaches for introgressing resistance genes by breeding have obtained limited success in counteracting the virus menace. Genetic modification (GM)-based strategies have been successful in engineering artificial resistance in plants. Several different approaches based on pathogen-derived resistance, antisense constructs, hairpin RNAs, double-stranded RNA, etc., have been used to enhance plants’ resistance to viruses. Recently, genome editing (GE) strategies mainly involving the CRISPR/Cas-mediated modifications are being used for virus control. In this review, we discuss the developments and advancements in GM- and GE-based methods for tackling viral infection in plants.
Dissecting Diagnostic and Management Strategies for Plant Viral Diseases: What Next?
Authors: Devi B. Megala , Guruprasath Samyuktha , Balu Pooraniammal , Chattopadhyay Anirudha , Thilagar Siva Sudha , Dhanabalan Kanaga Vijayan , Choudhary Manoj , Moparthi Swarnalatha , Jailani A. Abdul Kader
Published: February 2024

Recent advancements in molecular biology have revolutionized plant disease diagnosis and management. This review focuses on disease diagnosis through serological techniques, isothermal amplification methods, CRISPR-based approaches, and management strategies using RNA-based methods. Exploring high-throughput sequencing and RNA interference (RNAi) technologies like host-induced gene silencing (HIGS) and spray-induced gene silencing (SIGS), this review delves into their potential. Despite the precision offered by RNAi in pest and pathogen management, challenges such as off-target effects and efficient dsRNA delivery persist. This review discusses the significance of these strategies in preventing aphid-mediated plant virus transmission, emphasizing the crucial role of meticulous dsRNA design for effective viral RNA targeting while minimizing harm to plant RNA. Despite acknowledged challenges, including off-target effects and delivery issues, this review underscores the transformative potential of RNA-based strategies in agriculture. Envisaging reduced pesticide dependency and enhanced productivity, these strategies stand as key players in the future of sustainable agriculture.
Ability of Nutrient Management and Molecular Physiology Advancements to Overcome Abiotic Stress: A Study on Sub-Saharan African Crops
Authors: Kouame Koffi Pacome , Agrahari Raj Kishan , Konjengbam Noren Singh , Koyama Hiroyuki , Kobayashi Yuriko
Published: February 2024

Abiotic stress is a major cause of the declining crop yield worldwide, especially in tropical agricultural areas. Meeting the global food demand has become a serious challenge, especially in tropical areas, because of soil acidity, Al and Fe toxicity, drought and heat stress, and climate change. In this article, we reviewed several research and review papers from Google Scholar to list the different solutions available for the mitigation of abiotic stress, especially in tropical regions where several major crops, such as maize, sorghum, wheat, rice, soybean, and millet, are affected by abiotic stress and fertilizer input. In particular, Sub-Saharan Africa (SSA) has been affected by the low use of fertilizers owing to their high cost. Therefore, soil and plant researchers and farmers have developed many techniques to mitigate the effects of stress and improve the crop yield based on the agroecological zone and crop type. Nutrient management using chemical fertilizers alone or in combination with organic crops is a strategy recommended to cope with abiotic stress and increase the crop yield, particularly in developing countries. Notably, integrated soil fertility management has been effective in semi-arid areas under drought and heat stress and in subhumid and humid areas with high soil acidity and Fe toxicity in Africa. Recent advances in the molecular physiology of various crops considered a staple food in SSA have facilitated the breeding of transgenic tolerant plants with high yield. However, the feasibility and implementation of this technique in the African continent and most tropical developing countries are major issues that can be solved via adequate subsidies and support to farmers. This review can aid in the development of novel strategies to decrease hunger and food insecurity in SSA.
AI-Based Prediction of Carrot Yield and Quality on Tropical Agriculture
Authors: de Lima Silva Yara Karine , Furlani Carlos Eduardo Angeli , Canata Tatiana Fernanda
Published: February 2024

The adoption of artificial intelligence tools can improve production efficiency in the agroindustry. Our objective was to perform the predictive modeling of carrot yield and quality. The crop was grown in two commercial areas during the summer season in Brazil. The root samples were taken at 200 points with a 30 × 30 m sampling grid at 82 and 116 days after sowing in both areas. The total fresh biomass, aerial part, and root biometry were quantified for previous crop harvesting to measure yield. The quality of the roots was assessed by sub-sampling three carrots by the concentration of total soluble solids (°Brix) and firmness in the laboratory. Vegetation indices were extracted from satellite imagery. The most important variables for the predictive models were selected by principal component analysis and submitted to the Artificial Neural Network (ANN), Random Forest (RF), and Multiple Linear Regression (MLR) algorithms. SAVI and NDVI indices stood out as predictors of crop yield, and the results from the ANN (R2 = 0.68) were superior to the RF (R2 = 0.67) and MLR (R2 = 0.61) models. Carrot quality cannot be modeled by the predictive models in this study; however, it should be explored in future research, including other crop variables.
Advances in Nanomaterials and Composites Based on Mesoporous Materials as Antimicrobial Agents: Relevant Applications in Human Health
Authors: Gomez Germán E. , Hamer Mariana , Regiart Matías D. , Tortella Gonzalo R. , Seabra Amedea B. , Soler Illia Galo J. A. A. , Fernández-Baldo Martín A.
Published: February 2024

Nanotechnology has emerged as a cornerstone in contemporary research, marked by the advent of advanced technologies aimed at nanoengineering materials with diverse applications, particularly to address challenges in human health. Among these challenges, antimicrobial resistance (AMR) has risen as a significant and pressing threat to public health, creating obstacles in preventing and treating persistent diseases. Despite efforts in recent decades to combat AMR, global trends indicate an ongoing and concerning increase in AMR. The primary contributors to the escalation of AMR are the misuse and overuse of various antimicrobial agents in healthcare settings. This has led to severe consequences not only in terms of compromised treatment outcomes but also in terms of substantial financial burdens. The economic impact of AMR is reflected in skyrocketing healthcare costs attributed to heightened hospital admissions and increased drug usage. To address this critical issue, it is imperative to implement effective strategies for antimicrobial therapies. This comprehensive review will explore the latest scientific breakthroughs within the metal–organic frameworks and the use of mesoporous metallic oxide derivates as antimicrobial agents. We will explore their biomedical applications in human health, shedding light on promising avenues for combating AMR. Finally, we will conclude the current state of research and offer perspectives on the future development of these nanomaterials in the ongoing battle against AMR.
NADPH Oxidase 3: Beyond the Inner Ear
Authors: Herb Marc
Published: February 2024

Reactive oxygen species (ROS) were formerly known as mere byproducts of metabolism with damaging effects on cellular structures. The discovery and description of NADPH oxidases (Nox) as a whole enzyme family that only produce this harmful group of molecules was surprising. After intensive research, seven Nox isoforms were discovered, described and extensively studied. Among them, the NADPH oxidase 3 is the perhaps most underrated Nox isoform, since it was firstly discovered in the inner ear. This stigma of Nox3 as “being only expressed in the inner ear” was also used by me several times. Therefore, the question arose whether this sentence is still valid or even usable. To this end, this review solely focuses on Nox3 and summarizes its discovery, the structural components, the activating and regulating factors, the expression in cells, tissues and organs, as well as the beneficial and detrimental effects of Nox3-mediated ROS production on body functions. Furthermore, the involvement of Nox3-derived ROS in diseases progression and, accordingly, as a potential target for disease treatment, will be discussed.
Formyl Peptide Receptor 2-Dependent cPLA2 and 5-LOX Activation Requires a Functional NADPH Oxidase
Authors: Pecchillo Cimmino Tiziana , Panico Iolanda , Scarano Simona , Stornaiuolo Mariano , Esposito Gabriella , Ammendola Rosario , Cattaneo Fabio
Published: February 2024

Phospholipases (PL) A2 catalyzes the hydrolysis of membrane phospholipids and mostly generates arachidonic acid (AA). The enzyme 5-lipoxygenase (5-LOX) can metabolize AA to obtain inflammatory leukotrienes, whose biosynthesis highly depends on cPLA2 and 5-LOX activities. Formyl Peptide Receptor 2 (FPR2) belongs to a subfamily of class A GPCRs and is considered the most versatile FPRs isoform. Signaling triggered by FPR2 includes the activation of several downstream kinases and NADPH oxidase (NOX)-dependent ROS generation. In a metabolomic analysis we observed a significant increase in AA concentration in FPR2-stimulated lung cancer cell line CaLu-6. We analyzed cPLA2 phosphorylation and observed a time-dependent increase in cPLA2 Ser505 phosphorylation in FPR2-stimulated cells, which was prevented by the MEK inhibitor (PD098059) and the p38MAPK inhibitor (SB203580) and by blocking NOX function. Similarly, we demonstrated that phosphorylation of 5-LOX at Ser271 and Ser663 residues requires FPR2-dependent p38MAPK and ERKs activation. Moreover, we showed that 5-LOX Ser271 phosphorylation depends on a functional NOX expression. Our overall data demonstrate for the first time that FPR2-induced ERK- and p38MAPK-dependent phosphorylation/activation of cPLA2 and 5-LOX requires a functional NADPH oxidase. These findings represent an important step towards future novel therapeutic possibilities aimed at resolving the inflammatory processes underlying many human diseases.
Direct Numerical Simulation of Turbulent Boundary Layer over Cubical Roughness Elements
Authors: Yoon Min
Published: February 2024

The present study explores turbulence statistics in turbulent flow over urban-like terrain using direct numerical simulation (DNS). DNS is performed in a turbulent boundary layer (TBL) over 3D cubic roughness elements. The turbulence statistics at Reτ = 816 are compared with those of experimental and numerical studies for validation, where Reτ is the friction Reynolds number. The flow exhibits wake interference characteristics similar to k-type roughness. Logarithmic variations in streamwise and spanwise Reynolds stresses and a plateau in Reynolds shear stress are observed, reminiscent of Townsend’s attached-eddy hypothesis. The energy at long wavelengths near the top of elements extends to smaller scales, indicating a two-scale behavior and a potential link to amplitude modulation. The quadrant analysis of Reynolds shear stress is employed, revealing significant changes in the contributions of ejection and sweep events near the top of elements. The results of quadrant analysis in the outer region closely resemble those of a TBL over a smooth wall, aligning with Townsend’s outer-layer similarity. The analysis of the transport equation of turbulent kinetic energy highlights the role of the roughness elements in energy transfer, especially pressure transport. Streamwise energy is mainly reduced near upstream elements and redirected in other directions.
Towards Greener Packaging: Tapioca Starch-Based Biocomposites with Siam Weed Extract and Flax Seed Gel as Sustainable Antibacterial Packaging Material
Authors: Swapna Mohanachandran Nair Sindhu , Oscar Abin , Korte Dorota , Sankararaman Sankaranarayana Iyer
Published: February 2024

This paper delves into the development of biocomposite (BC) packaging material from tapioca starch (TS), flax seed gel (FS), and Siam weed (SW) extract, considering the increased demand for sustainable, eco-friendly packaging materials. The BCs—BC1 (40 mL), BC2 (80 mL), and BC3 (120 mL), prepared by varying the concentration of SW—were subjected to morphological, structure, thermal, and optical characterisations. The BC with a fragmented, agglomerated morphology shows cellulosic peaks in the X-ray diffraction pattern, indicating the C-type crystalline structure in TS. Thermogravimetric analysis confirmed the BC’s safe use up to 300 °C, with a minimal 40% weight loss. Differential scanning calorimetry plots identified heat absorption during gelatinisation, with an endothermic peak at 300 °C marking a phase transition. The Fourier transform infrared (FTIR) and UV–visible spectra revealed functional groups that attribute antibacterial potential to the BC. The optical analyses show greater absorption and fewer emissions, resulting in the increased enthalpy responsible for the microbial activities. Antibacterial studies demonstrated BC2’s efficacy against Staphylococcus aureus, while the stability against humidity and the minimal weight loss underscored the BC’s robust thermal stability. FTIR spectra of post-heating at 80 °C confirmed the structural integrity, positioning the BC as a promising material for eco-friendly packaging solutions.
Micellar and Solvent Loan Chemical Extraction as a Tool for the Development of Natural Skin Care Cosmetics Containing Substances Isolated from Grapevine Buds
Authors: Hordyjewicz-Baran Zofia , Wasilewski Tomasz , Zarębska Magdalena , Stanek-Wandzel Natalia , Zajszły-Turko Ewa , Tomaka Magdalena , Zagórska-Dziok Martyna
Published: February 2024

The present study aimed to evaluate the possibility of using micellar and solvent loan chemical extraction (LCE) to isolate valuable components from grapevine leaf buds, subsequently utilizing them to prepare functional and safe-to-use cosmetic preparations, specifically facial serums. An aqueous solution of polyglyceryl-4 laurate/sebacate and polyglyceryl-6 caprylate/caprate was employed for a micellar LCE, while an aqueous solution of 1,3-propanediol was used for a solvent LCE. Importantly, the extraction medium was exclusively comprised of components from the designed final cosmetic product. Consequently, no additional substances were present in the cosmetics developed, and the formulation was notably enhanced by compounds extracted from grapevine buds. The antioxidant properties and compound characterization of the obtained micellar (SurfE) and solvent (SolvE) extracts based on grapevine buds were tested and compared. UPLC-MS/MS results indicated that the extracts were rich in phenolic and flavonoid compounds, exhibiting antioxidant activity as measured using the DPPH and ABTS scavenging ability. The extracts were used to prepare model facial serums, which underwent evaluation based on fundamental functionality-related parameters (e.g., rheological characteristics and color) and their impact on the skin through cytotoxicity assessment. The results demonstrated that facial serums with extracts based on grapevine buds provided safe, natural cosmetics.