bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Soil Microbes on Forest Recovery to Climax Community through the Regulation of Nitrogen Cycling
Authors: Qi Dandan , Feng Fujuan , Fu Yanmei , Ji Ximei , Liu Xianfa
Published: September 2020

Microbes, as important regulators of ecosystem processes, play essential roles in ecosystem recovery after disturbances. However, it is not clear how soil microbial communities and functions change and affect forest recovery after clear-cutting. Here, we used metagenome sequencing to systematically analyse the differences in soil microbial community composition, functions, and nitrogen (N) cycling pathways between primary Korean pine forests (PF) and secondary broad-leaved forests (SF) formed after clear-cutting. Our results showed that the dominant phyla of the two forest types were consistent, but the relative abundance of some phyla was significantly different. Meanwhile, at the genus level, the fold-changes of rare genera were larger than the dominant and common genera. The genes related to microbial core metabolic functions, virulence factors, stress response, and defence were significantly enriched in SF. Additionally, based on the relative abundance of functional genes, a schema was proposed to analyse the differences in the whole N cycling processes between the two forest types. In PF, the stronger ammoniation and dissimilatory nitrate reduction (DNRA) and the weaker nitrification provided a genetic explanation for PF dominated by ammonium (NH4+) rather than nitrate (NO3−). In SF, the weaker DNRA, the stronger nitrification and denitrification, the higher soil available phosphorus (AP), and the lower nitrogen to phosphorus ratio (N/P) comprehensively suggested that SF was faced with a greater degree of N limitation. These results offer insights into the potential relationship between soil microbes and forest recovery, and aid in implementing proper forestry management.
Latitudinal Diversity Gradients and Rapoport Effects in Chinese Endemic Woody Seed Plants
Authors: Liu Hua , Yu Ruoyun , Huang Jihong , Liu Yibo , Zang Runguo , Guo Zhongjun , Ding Yi , Lu Xinghui , Li Qian , Chen Han Y. H.
Published: September 2020

The distribution of plant species diversity has long been a major focus on biogeography. Yet, the universality of the popular Rapoport’s rule remains controversial for endemic plants, as previous studies have focused more on broad-ranged species. Here, we collected data for 4418 endemic woody seed plant species across China, including trees, shrubs, and lianas, to explore the latitudinal patterns of species range size and richness, and test the relevant biogeographic law. The species range size distribution was examined for conformity with Rapoport’s rule using four methods (i.e., Steven’s, Pagel’s, the mid-point, and the across-species method). Spatial patterns of species richness along latitudinal gradient were also investigated by parabolic regression. Results showed that species range size increased with latitude for all species as well as by trees, shrubs and lianas, especially assessed by Pagel’s method. Species richness was highest at low latitude, where species range size was smallest, and decreased with increasing latitude. The species range size and richness of shrubs were maximum, followed by trees then lianas. These findings prove that Rapoport’s rule is strongly supported by latitudinal patterns of species distribution in Chinese endemic woody seed plants.
Soil Enzyme Activity and Soil Nutrients Jointly Influence Post-Fire Habitat Models in Mixed-Conifer Forests of Yosemite National Park, USA
Authors: Tamjidi Jelveh , Lutz James A.
Published: September 2020

Disentangling the relative importance of habitat filtering and dispersal limitations at local scales (<1 km2) in shaping species composition remains an important question in community ecology. Previous studies have examined the relative importance of these mechanisms using topography and selected soil properties. We examined both topography and edaphic properties from 160 locations in the recently burned 25.6 ha Yosemite Forest Dynamics Plot (YFDP) in Yosemite National Park, California, USA. In addition to eight soil chemical properties, we included phosphatases and urease enzymes in a definition of habitat niches, primarily because of their rapid changes with fire (compared to soil nutrients) and also their role in ecosystem function. We applied environmental variables to the distributions of 11 species. More species–habitat associations were defined by soil properties (54.5%) than topographically-defined habitat (45.4%). We also examined the relative importance of spatial and environmental factors in species assemblage. Proportions explained by spatial and environmental factors differed among species and demographic metrics (stem abundance, basal area increment, mortality, and recruitment). Spatial factors explained more variation than environmental factors in stem abundance, mortality, and recruitment. The contributions of urease and acid phosphatase to habitat definition were significant for species abundance and basal area increment. These results emphasize that a more complete understanding of niche parameters is needed beyond simple topographic factors to explain species habitat preference. The stronger contribution of spatial factors suggests that dispersal limitation and unmeasured environmental variables have high explanatory power for species assemblage in this coniferous forest.
Valorization of Spent Coffee Grounds, Biochar and other residues to Produce Lightweight Clay Ceramic Aggregates Suitable for Nursery Grapevine Production
Authors: Ronga Domenico , Parisi Mario , Barbieri Luisa , Lancellotti Isabella , Andreola Fernanda , Bignami Cristina
Published: September 2020

The valorization of agro-industrial by-products is one of the key strategies to improve agricultural sustainability. In the present study, spent coffee grounds and biochar were used as pore forming agents in the realization of lightweight clay ceramic aggregates that were used as sustainable fertilizers, in addition to tailored glass fertilizer containing phosphorous (P) and potassium (K) and nitrogen (N) synthetic fertilizer, for nursery grapevine production. The obtained fertilizers were assessed in a pot experiment for the fertilization of bare-rooted vines. Unfertilized (T0) and fertilized plants (T1, using NPK-containing commercial fertilizer) were used as controls. Plants fertilized by spent coffee grounds and spent coffee grounds + biochar-containing lightweight aggregates and added with 30 wt% of the above-mentioned glass and N fertilizers (T2 and T3, respectively) recorded higher values of plant height, shoot diameter, leaf and node numbers. Moreover, T2 treatment induced the highest chlorophyll content, shoot and root dry weights. The present study shows that lightweight clay ceramic aggregates containing spent coffee grounds and glass and N fertilizers can be used for nursery grapevine production, in turn improving the agricultural sustainability.
Are Advanced Glycation End Products in Skin Associated with Vascular Dysfunction Markers? A Meta-Analysis
Authors: Saz-Lara Alicia , Álvarez-Bueno Celia , Martínez-Vizcaíno Vicente , Notario-Pacheco Blanca , Sequí-Dominguez Irene , Cavero-Redondo Iván
Published: September 2020

Evidence exists regarding the association between advanced glycation end products and different cardiovascular disease subclinical processes, such as arterial stiffness and atherosclerosis. With this systematic review and meta-analysis, we aimed to provide a synthesis of the evidence regarding the association of arterial stiffness measured by pulse wave velocity and atherosclerosis measured by carotid intima media thickness with skin autofluorescence. A systematic search was performed using: MEDLINE (PubMed), SCOPUS, and Web of Science, until 30 March 2020. Cross-sectional studies or baseline data from prospective longitudinal studies were considered. The DerSimonian and Laird method was used to calculate the pooled estimates of correlation coefficients and the corresponding 95% confidence intervals (CI) for the association of pulse wave velocity and carotid intima media thickness with skin autofluorescence. Twenty-five studies were included in the systematic review and meta-analysis, including 6306 subjects. The pooled correlation coefficient was 0.25 (95% CI: 0.18, 0.31) for pulse wave velocity and skin autofluorescence, and 0.31 (95% CI: 0.25, 0.38) for carotid intima media thickness and skin autofluorescence. This systematic review and meta-analysis provide a synthesis of the evidence showing a positive weak association of pulse wave velocity and carotid intima media thickness with skin autofluorescence.
Exploring Social and Financial Hardship, Mental Health Problems and the Role of Social Support in Asylum Seekers Using Structural Equation Modelling
Authors: Sengoelge Mathilde , Solberg Øivind , Nissen Alexander , Saboonchi Fredrik
Published: September 2020

Asylum seekers are exposed to a range of social and financial difficulties suggested to adversely impact mental health. Uprooted social networks and living conditions during the asylum seeking process potentially predispose this population to low access to social support. The aim of this study was to examine the relationship between social and financial hardship and mental health problems, and assess the potential mediating role of social support among asylum seekers. Cross sectional survey data from a cohort of asylum seekers in Sweden (N = 455) were subjected to structural equation modelling for examining hypothesized pathways between social and financial hardship, common mental health problems (CMHPs) and social support. Fit indices showed adequate to excellent fit of the examined models with CMHPs as the outcome (all CFI ≥ 0.951, RMSEA < 0.05, SRMR < 0.056). CMHPs were positively regressed on social and financial hardship (B = 0.786, S = 0.102, p < 0.001) and negatively regressed on social support (B = −0.103, SE = 0.032, p = 0.001). Social support mediated the association between social and financial hardship and CMHPs (effect estimate = 0.075, 95% CI = 0.032–0.136). The results point to the importance of social and living conditions of asylum seekers and indicate that social support is both socially patterned and may act as a mitigating resource to inform interventions and policies.
Erratum: Meyer, J., et al. Changes in Physical Activity and Sedentary Behavior in Response to COVID-19 and Their Associations with Mental Health in 3052 US Adults. Int. J. Environ. Res. Public Health 2020, 17(18), 6469
Authors: Meyer Jacob , McDowell Cillian , Lansing Jeni , Brower Cassandra , Smith Lee , Tully Mark , Herring Matthew
Published: September 2020

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Conceptualization, Development and Psychometric Evaluations of a New Medication-Related Health Literacy Instrument: The Chinese Medication Literacy Measurement
Authors: Lin Hsiang-Wen , Chang Elizabeth H. , Ko Yu , Wang Chun-Yu , Wang Yu-Shan , Mafruhah Okti Ratna , Wu Shang-Hua , Chen Yu-Chieh , Huang Yen-Ming
Published: September 2020

There is a need for valid and reliable instruments to focus on medication aspects of health literacy and help healthcare professionals address patients’ barriers to medication use. This cross-sectional study describes the conceptualization, development, and psychometric properties of the first Chinese Medication Literacy Measurement (ChMLM) to assess the level of health literacy on medication use. The 17-item ChMLM (ChMLM-17) and its short form, 13-item ChMLM (ChMLM-13), consist of four sections (vocabulary, over-the-counter labels, prescription labels, and advertisements) to cover six domains of medication-related health literacy. Multistage stratified quota sampling was attempted to recruit a representative sample in Taiwan. Receiver operating characteristic curves were used to identify the cut-off point for differentiating high and low medication literacy. Psychometric analyses were performed (n = 1410) to assess the reliability and validity separately on all samples and sociodemographic subgroups. The 17- and 13-item versions both had high construct validity among all patients and patients with low medication literacy. The developed ChMLM-17 and ChMLM-13 is expected to help healthcare providers and researchers to accurately measure medication-related health literacy and improve medication use in the real-world practice.
Preliminary Analysis of Relationships between COVID19 and Climate, Morphology, and Urbanization in the Lombardy Region (Northern Italy)
Authors: Fazzini Massimiliano , Baresi Claudia , Bisci Carlo , Bna Claudio , Cecili Alessandro , Giuliacci Andrea , Illuminati Sonia , Pregliasco Fabrizio , Miccadei Enrico
Published: September 2020

The coronavirus disease 2019 (COVID-19) pandemic is the most severe global health and socioeconomic crisis of our time, and represents the greatest challenge faced by the world since the end of the Second World War. The academic literature indicates that climatic features, specifically temperature and absolute humidity, are very important factors affecting infectious pulmonary disease epidemics - such as severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS); however, the influence of climatic parameters on COVID-19 remains extremely controversial. The goal of this study is to individuate relationships between several climate parameters (temperature, relative humidity, accumulated precipitation, solar radiation, evaporation, and wind direction and intensity), local morphological parameters, and new daily positive swabs for COVID-19, which represents the only parameter that can be statistically used to quantify the pandemic. The daily deaths parameter was not considered, because it is not reliable, due to frequent administrative errors. Daily data on meteorological conditions and new cases of COVID-19 were collected for the Lombardy Region (Northern Italy) from 1 March, 2020 to 20 April, 2020. This region exhibited the largest rate of official deaths in the world, with a value of approximately 1700 per million on 30 June 2020. Moreover, the apparent lethality was approximately 17% in this area, mainly due to the considerable housing density and the extensive presence of industrial and craft areas. Both the Mann–Kendall test and multivariate statistical analysis showed that none of the considered climatic variables exhibited statistically significant relationships with the epidemiological evolution of COVID-19, at least during spring months in temperate subcontinental climate areas, with the exception of solar radiation, which was directly related and showed an otherwise low explained variability of approximately 20%. Furthermore, the average temperatures of two highly representative meteorological stations of Molise and Lucania (Southern Italy), the most weakly affected by the pandemic, were approximately 1.5 °C lower than those in Bergamo and Brescia (Lombardy), again confirming that a significant relationship between the increase in temperature and decrease in virulence from COVID-19 is not evident, at least in Italy.
Syk Inhibitors: New Computational Insights into Their Intraerythrocytic Action in Plasmodium falciparum Malaria
Authors: Marchetti Giuseppe , Dessì Alessandro , Dallocchio Roberto , Tsamesidis Ioannis , Pau Maria Carmina , Turrini Francesco Michelangelo , Pantaleo Antonella
Published: September 2020

Resistance to antimalarial drugs has spread rapidly over the past few decades. The WHO recommends artemisinin-based combination therapies for the treatment of uncomplicated malaria, but unfortunately these approaches are losing their efficacy in large areas of Southeast Asia. In 2016, artemisinin resistance was confirmed in 5 countries of the Greater Mekong subregion. We focused our study on Syk inhibitors as antimalarial drugs. The Syk protein is present in human erythrocytes, and the membrane of protein band 3 is its major target following activation by oxidant stress. Tyr phosphorylation of band 3 occurs during P. falciparum growth, leading to the release of microparticles containing hemicromes and structural weakening of the host cell membrane, simplifying merozoite reinfection. Syk inhibitors block these events by interacting with the Syk protein’s catalytic site. We performed in vitro proteomics and in silico studies and compared the results. In vitro studies were based on treatment of the parasite’s cellular cultures with different concentrations of Syk inhibitors, while proteomics studies were focused on the Tyr phosphorylation of band 3 by Syk protein with the same concentrations of drugs. In silico studies were based on different molecular modeling approaches in order to analyze and optimize the ligand–protein interactions and obtain the highest efficacy in vitro. In the presence of Syk inhibitors, we observed a marked decrease of band 3 Tyr phosphorylation according to the increase of the drug’s concentration. Our studies could be useful for the structural optimization of these compounds and for the design of novel Syk inhibitors in the future.
Interpreting Morphological Adaptations Associated with Viviparity in the Tsetse Fly Glossina morsitans (Westwood) by Three-Dimensional Analysis
Authors: Attardo Geoffrey M , Tam Nicole , Parkinson Dula , Mack Lindsey K , Zahnle Xavier J , Arguellez Joceline , Takáč Peter , Malacrida Anna R
Published: September 2020

Tsetse flies (genus Glossina), the sole vectors of African trypanosomiasis, are distinct from most other insects, due to dramatic morphological and physiological adaptations required to support their unique biology. These adaptations are driven by demands associated with obligate hematophagy and viviparous reproduction. Obligate viviparity entails intrauterine larval development and the provision of maternal nutrients for the developing larvae. The reduced reproductive capacity/rate associated with this biology results in increased inter- and intra-sexual competition. Here, we use phase contrast microcomputed tomography (pcMicroCT) to analyze morphological adaptations associated with viviparous biology. These include (1) modifications facilitating abdominal distention required during blood feeding and pregnancy, (2) abdominal and uterine musculature adaptations for gestation and parturition of developed larvae, (3) reduced ovarian structure and capacity, (4) structural features of the male-derived spermatophore optimizing semen/sperm delivery and inhibition of insemination by competing males and (5) structural features of the milk gland facilitating nutrient incorporation and transfer into the uterus. Three-dimensional analysis of these features provides unprecedented opportunities for examination and discovery of internal morphological features not possible with traditional microscopy techniques and provides new opportunities for comparative morphological analyses over time and between species.
Morphological Characteristics of the Osteoplastic Potential of Synthetic CaSiO3/HAp Powder Biocomposite
Authors: Apanasevich Vladimir , Papynov Evgeniy , Plekhova Nataliay , Zinoviev Sergey , Kotciurbii Evgeniy , Stepanyugina Alexandra , Korshunova Oksana , Afonin Igor , Evdokimov Ivan , Shichalin Oleg , Bardin Artem , Nevozhai Vladimir , Polezhaev Alexandr
Published: September 2020

The study describes the influence of synthetic CaSiO3/HAp powder biocomposite on the process of regeneration in osseous tissue in the alveolar ridges in terms of the morphological characteristics of the osteoplastic potential. The authors investigated the osteoinduction and osteoconduction “in vivo” processes during bone tissue regeneration in the mandible defect area of an experimental animal (rabbit). The possibility of angiogenesis in the graft as an adaptation factor was studied in the process of bone tissue regeneration. The results of the histological study that included the qualitative parameters of bone tissue regeneration, the morphometric parameters (microarchitectonics) of the bone, the parameters of osteosynthesis (thickness of the osteoid plates), and resorption (volume density of the eroded surface) were presented. The results allowed the authors to characterize the possibility of the practical adaptation for synthetic powder biocomposite as an osteoplastic graft for the rehabilitation of osseous defects in dentistry.
Mechanical Properties of Corner Joints Made of Honeycomb Panels with Double Arrow-Shaped Auxetic Cores
Authors: Majewski Adam , Krystofiak Tomasz , Smardzewski Jerzy
Published: September 2020

The development of both light and strong wood-derived materials is an interesting research area, particularly in terms of usability in, e.g., furniture constructions. Honeycomb panels being current industry standard are relatively thick (32 mm and 40 mm), thus their attractiveness in designing furniture is limited. In a few studies, it has been shown that honeycomb panels with paper cores are characterized by unsatisfactory mechanical properties, especially when the composite thickness is less than 20 mm. From the literature, it is also evident that mechanical properties might be improved by introducing auxetic features into the core structure. Even though it is a concept with great potential, there are a few studies dealing with honeycomb panels with auxetic cores made of paper. Furthermore, there is no research on the corner joints made from such material. For this reason, the aim of the study was to test the bending behavior of the corner adhesive joints made of honeycomb panels with double arrow-shaped auxetic cores. Within the research, the core cell was adopted based on literature and preliminary studies, paper auxetic cores were produced by the use of the designed and 3d printed device, and joints stiffness and strength were calculated analytically based on the experiment results. Evaluated corner joints stiffness, both in compression and tension test, is greater for samples made of panels with designed auxetic cores. Surprisingly, in the analyzed range of elasticity, it was statistically proved that the values of joint stiffness coefficient K did not vary significantly between compared joints pairs.
Porous Carbon-Based Supercapacitors Directly Derived from Metal–Organic Frameworks
Authors: Kim Hyun-Chul , Huh Seong
Published: September 2020

Numerously different porous carbons have been prepared and used in a wide range of practical applications. Porous carbons are also ideal electrode materials for efficient energy storage devices due to their large surface areas, capacious pore spaces, and superior chemical stability compared to other porous materials. Not only the electrical double-layer capacitance (EDLC)-based charge storage but also the pseudocapacitance driven by various dopants in the carbon matrix plays a significant role in enhancing the electrochemical supercapacitive performance of porous carbons. Since the electrochemical capacitive activities are primarily based on EDLC and further enhanced by pseudocapacitance, high-surface carbons are desirable for these applications. The porosity of carbons plays a crucial role in enhancing the performance as well. We have recently witnessed that metal–organic frameworks (MOFs) could be very effective self-sacrificing templates, or precursors, for new high-surface carbons for supercapacitors, or ultracapacitors. Many MOFs can be self-sacrificing precursors for carbonaceous porous materials in a simple yet effective direct carbonization to produce porous carbons. The constituent metal ions can be either completely removed during the carbonization or transformed into valuable redox-active centers for additional faradaic reactions to enhance the electrochemical performance of carbon electrodes. Some heteroatoms of the bridging ligands and solvate molecules can be easily incorporated into carbon matrices to generate heteroatom-doped carbons with pseudocapacitive behavior and good surface wettability. We categorized these MOF-derived porous carbons into three main types: (i) pure and heteroatom-doped carbons, (ii) metallic nanoparticle-containing carbons, and (iii) carbon-based composites with other carbon-based materials or redox-active metal species. Based on these cases summarized in this review, new MOF-derived porous carbons with much enhanced capacitive performance and stability will be envisioned.
Ciprofloxacin and Graphene Oxide Combination—New Face of a Known Drug
Authors: Matulewicz Karolina , Kaźmierski Łukasz , Wiśniewski Marek , Roszkowski Szymon , Roszkowski Krzysztof , Kowalczyk Oliwia , Roy Archi , Tylkowski Bartosz , Bajek Anna
Published: September 2020

Drug modification with nanomaterials is a new trend in pharmaceutical studies and shows promising results, especially considering carbon-based solutions. Graphene and its derivatives have attracted much research interest for their potential applications in biomedical areas as drug modifiers. The following work is a comprehensive study regarding the toxicity of ciprofloxacin (CIP) modified by graphene oxide (GO). The influence on the morphology, viability, cell death pathway and proliferation of T24 and 786-0 cells was studied. The results show that ciprofloxacin modified with graphene oxide (CGO) shows the highest increase in cytotoxic potential, especially in the case of T24 cells. We discovered a clear connection between CIP modification with GO and the increase in its apoptotic potential. Our results show that drug modification with carbon-based nanomaterials might be a promising strategy to improve the qualities of existing drugs. Nevertheless, it is important to remember that cytotoxicity effects are highly dependent on dose and nanomaterial size. It is necessary to conduct further research to determine the optimal dose of GO for drug modification.
Laboratory Investigation of Aging Resistance for Rubberized Bitumen Modified by Using Microwave Activation Crumb Rubber and Different Modifiers
Authors: Zhang Ben , Chen Huaxin , Zhang Honggang , Wu Yongchang , Kuang Dongliang , Guo Fengjun
Published: September 2020

Different modification methods, such as adding modifiers and pretreating crumb rubber, have been developed to achieve decent engineering properties and reduce the viscosity of rubberized bitumen. This study evaluated the influence of the modification methods on the aging resistance for rubberized bitumen. Two types of crumb rubber—a 40-mesh crumb rubber and a microwave-pretreated crumb rubber—and two kinds of modifiers—Sasobit and Trans-polyoctenamer—were selected to prepare rubberized bitumen. The samples were subjected to a Thin-Film Oven Test for the simulation of the short-term aging condition, while a Pressure-Aging-Vessel test was used to simulate the long-term aging condition. The indexes of rubberized bitumen, including softening point, elastic recovery ratio, maximum load, ductility, fracture energy, phase angle, and dynamic modulus, were tested before and after aging. The result showed that trans-polyoctenamer displayed the best resistance to short-term aging, while Sasobit significantly improved the fracture energy of rubberized bitumen after short-term aging. Microwave pretreated partially destroyed the internal structure of crumb rubber, leading to a decrease of short-term aging resistance for rubberized bitumen. Compared with short-term aging, the changing trends of various indexes were basically same, except the discrepancy of properties indexes was reduced after long-term aging.
A Digital Twin Approach to a Quantitative Microstructure-Property Study of Carbon Fibers through HRTEM Characterization and Multiscale FEA
Authors: Sweat Rebekah , Park Jin Gyu , Liang Richard
Published: September 2020

Microstructures of typical carbon fibers (CFs) from polyacrylonitrile (PAN) and pitch-based precursors were studied using a novel digital twin approach with individual carbon fibers for a local crystal scale model. The transmission electron microscopy (TEM) samples were prepared using a focused-ion beam (FIB) for both longitudinal and transverse directions of carbon fibers. Measurements of the crystal size and orientation were estimated from X-ray scattering. TEM imaging of graphitic packing facilitated further comprehension of associations between processing and final material properties, which could enable customization of microstructures for property targets. Then the detailed microstructural information and their X-ray scattering properties were incorporated into the simulation model of an individual carbon fiber. Assuming that graphene properties are the same among different forms of carbon fiber, a reasonable physics-based explanation for such a drastic decrease in strength is the dislocations between the graphitic units. The model reveals critical defects and uncertainty of carbon fiber microstructures, including skin/core alignment differences and propagating fracture before ultimate failure. The models are the first to quantify microstructures at the crystal scale with micromechanics and to estimate tensile and compressive mechanical properties of carbon fiber materials, as well as potentially develop new fundamental understandings for tailoring carbon fiber and composites properties.
In Vitro Study of Comparative Evaluation of Marginal and Internal Fit between Heat-Pressed and CAD-CAM Monolithic Glass-Ceramic Restorations after Thermal Aging
Authors: Vasiliu Roxana-Diana , Porojan Sorin Daniel , Porojan Liliana
Published: September 2020

The accuracy of newly developed ceramic materials is still being studied. Marginal and internal adaptation are known factors that have an essential impact on the long term success of dental restorations. The aim of this in vitro study was to evaluate the marginal and internal fit of heat-pressed and milled monolithic glass-ceramic restorations based on their ceramic type, processing technique, and in vitro thermocycling. Thirty-two crowns were studied and divided into four groups (n = 8), according to the ceramic material (feldspathic glass-ceramic (F) and zirconia reinforced lithium silicate glass-ceramic (ZLS)) and to their technological obtaining processes (milling (M) and heat-pressing (P)). A typodont preparation was scanned with a D2000 3D scanner to obtain identical 32 resin 3D-printed abutment teeth. Marginal and internal gaps were measured using the silicone replica technique under 40× magnification. The crowns were further cemented and thermally aged for 10,000 cycles After cementation and thermocycling of the samples, marginal and internal gaps were assessed using micro-CT (micro-computed tomography)) analysis. Data were statistically analyzed using statistical tests. Significant differences were found before and after cementation and thermocycling among the tested materials (p < 0.05). Related to technological processing, significant differences were seen in the marginal area between FP and FM (p < 0.05) Significant differences were also found in the axial and occlusal areas between the ZLSP and ZLSM. Thermocycling and cementation did not have a significant effect on the tested materials (p < 0.05). The technological processes influenced the marginal and internal fit of the crowns in favor of the CAD/CAM (computer aided design/computer aided manufacturing)technologies. Thermal aging had little effect on marginal adaptability; it increased the values for all the tested samples in a small way, but the values remained in their clinically acceptable range for all of the crowns.
A Novel Ramp Metering Approach Based on Machine Learning and Historical Data
Authors: Ghanbartehrani Saeed , Sanandaji Anahita , Mokhtari Zahra , Tajik Kimia
Published: September 2020

The random nature of traffic conditions on freeways can cause excessive congestion and irregularities in the traffic flow. Ramp metering is a proven effective method to maintain freeway efficiency under various traffic conditions. Creating a reliable and practical ramp metering algorithm that considers both critical traffic measures and historical data is still a challenging problem. In this study we use simple machine learning approaches to develop a novel real-time ramp metering algorithm. The proposed algorithm is computationally simple and has minimal data requirements, which makes it practical for real-world applications. We conduct a simulation study to evaluate and compare the proposed approach with an existing traffic-responsive ramp metering algorithm.
Solving Integro-Differential Boundary Value Problems Using Sinc-Derivative Collocation
Authors: Abdella Kenzu , Ross Glen
Published: September 2020

In this paper, the sinc-derivative collocation approach is used to solve second order integro-differential boundary value problems. While the derivative of the unknown variables is interpolated using sinc numerical methods, the desired solution and the integral terms are evaluated through numerical integration and all higher order derivatives are approximated through successive numerical differentiation. Suitable transformations are used to reduce non-homogeneous boundary conditions to homogeneous. Comparison of the proposed method with different approaches that were previously considered in the literature is carried out in order to test its accuracy and efficiency. The results show that the sinc-derivative collocation method performs well.
Non-Alcoholic Beverages, Old and Novel, and Their Potential Effects on Human Health, with a Focus on Hydration and Cardiometabolic Health
Authors: Sikalidis Angelos K. , Kelleher Anita H. , Maykish Adeline , Kristo Aleksandra S.
Published: September 2020

The Beverage Guidance System has established dietary recommendations for daily intake of commonly consumed beverages including water, tea, coffee, milk, non-calorically sweetened beverages, and calorically sweetened beverages. As obesity in America continues to be a growing problem, this guidance becomes of increasing importance due to many beverages’ potential links to Type 2 Diabetes Mellitus (T2DM), Cardiovascular disease (CVD), and numerous other harmful health effects. However, the growing popularity of “better for you” beverages is causing a shift in the market, with consumers pushing for healthier beverage alternatives. Beverages simultaneously present advantages while posing concerns that need to be evaluated and considered. In this review, health effects of nonalcoholic beverages are discussed including various aspects of consumption and current trends of the beverage market such as the novel Soft Seltzer category as an alternative to Hard Seltzer and various mashups. A variety of advisory boards and agencies responsible for dietary guidelines in various countries suggest drinking water as the preferred practice for hydration.
Single-Cell Elasticity Measurement with an Optically Actuated Microrobot
Authors: Grexa István , Fekete Tamás , Molnár Judit , Molnár Kinga , Vizsnyiczai Gaszton , Ormos Pál , Kelemen Lóránd
Published: September 2020

A cell elasticity measurement method is introduced that uses polymer microtools actuated by holographic optical tweezers. The microtools were prepared with two-photon polymerization. Their shape enables the approach of the cells in any lateral direction. In the presented case, endothelial cells grown on vertical polymer walls were probed by the tools in a lateral direction. The use of specially shaped microtools prevents the target cells from photodamage that may arise during optical trapping. The position of the tools was recorded simply with video microscopy and analyzed with image processing methods. We critically compare the resulting Young’s modulus values to those in the literature obtained by other methods. The application of optical tweezers extends the force range available for cell indentations measurements down to the fN regime. Our approach demonstrates a feasible alternative to the usual vertical indentation experiments.
Wafer-Level Vacuum-Packaged Translatory MEMS Actuator with Large Stroke for NIR-FT Spectrometers
Authors: Sandner Thilo , Gaumont Eric , Graßhoff Thomas , Rieck Andreas , Seifert Tobias , Auböck Gerald , Grahmann Jan
Published: September 2020

We present a wafer-level vacuum-packaged (WLVP) translatory micro-electro-mechanical system (MEMS) actuator developed for a compact near-infrared-Fourier transform spectrometer (NIR-FTS) with 800–2500 nm spectral bandwidth and signal-nose-ratio (SNR) > 1000 in the smaller bandwidth range (1200–2500 nm) for 1 s measuring time. Although monolithic, highly miniaturized MEMS NIR-FTSs exist today, we follow a classical optical FT instrumentation using a resonant MEMS mirror of 5 mm diameter with precise out-of-plane translatory oscillation for optical path-length modulation. Compared to highly miniaturized MEMS NIR-FTS, the present concept features higher optical throughput and resolution, as well as mechanical robustness and insensitivity to vibration and mechanical shock, compared to conventional FTS mirror drives. The large-stroke MEMS design uses a fully symmetrical four-pantograph suspension, avoiding problems with tilting and parasitic modes. Due to significant gas damping, a permanent vacuum of ≤3.21 Pa is required. Therefore, an MEMS design with WLVP optimization for the NIR spectral range with minimized static and dynamic mirror deformation of ≤100 nm was developed. For hermetic sealing, glass-frit bonding at elevated process temperatures of 430–440 °C was used to ensure compatibility with a qualified MEMS processes. Finally, a WLVP MEMS with a vacuum pressure of ≤0.15 Pa and Q ≥ 38,600 was realized, resulting in a stroke of 700 µm at 267 Hz for driving at 4 V in parametric resonance. The long-term stability of the 0.2 Pa interior vacuum was successfully tested using a Ne fine-leakage test and resulted in an estimated lifetime of >10 years. This meets the requirements of a compact NIR-FTS.
Characterization of Fine Fractions from the Processing of Municipal Solid Waste Incinerator Bottom Ashes for the Potential Recovery of Valuable Metals
Authors: Keber Sebastian , Schirmer Thomas , Elwert Tobias , Goldmann Daniel
Published: September 2020

Municipal waste incinerator bottom ashes contain copper contents comparable to those of low-grade ores in addition to other valuable metals. While the processing of coarse fractions (>2 mm) is state of the art, the fines with their residual metal content are landfilled. This paper presents the results from a mineralogical characterization of fine fractions from the processing of municipal solid waste incinerator bottom ashes. From the results, possible approaches for a recovery of copper from the fine fraction are derived. Spatially resolved phase analysis reveals that the material contains a very heterogenic mixture of naturally occurring compounds as well as particles of alloys, metals, artificial oxides, and sulfides. The most interesting element to recover is copper. Copper can be found in the form of alloys, simple sulfides (XS), and oxides (XO). During the incineration process, new phases are generated that differ from natural ones and therefore can be called artificial minerals. Additionally, several components are partially altered due to oxidation, especially after the prolonged outside storage of the bottom ash. Crystalline silicate and glass particles are only sporadically enriched in Cu. Based on these results, different processing techniques are discussed. Due to the small particle size distribution and the physical and physico-chemical properties of the particles, flotation seems to be the most promising technique for the enrichment of copper from municipal solid waste incineration bottom ash (MSWI-BA) fine fractions.
Become Competent within One Day in Generating Boxplots and Violin Plots for a Novice without Prior R Experience
Authors: Hu Kejin
Published: September 2020

The boxplot is a powerful visualization tool of sampled continuous data sets because of its rich information delivered, compact size, and effective visual expression. The advantage of boxplots is not widely appreciated. Many top journals suggest that boxplots should be used in place of bar charts, but have been wrongly replaced by bar charts. One technical barrier to the usage of boxplots in reporting quantitative data is that bench scientists are not competent in generating boxplots, and are afraid of R, a programming tool. This tutorial provides an effective training material in that even a novice without prior R experience can become competent, within one day, in generating professional boxplots. The available R scripts for boxplots are very limited in scope and are aimed at specialists, and the bench scientists have difficulty in following these scripts. This tutorial provides extensive step-by-step R scripts and instructions, as well as 29 illustrations for customizing every detail of the boxplot structures. Basic R commands and concepts are introduced for users without prior R experiences, which can be skipped by audiences with R knowledge. Violin plots are the enhanced version of boxplots, and therefore, this tutorial also provides a brief introduction and usage of the R package vioplot with one additional illustration. While the protocol is prepared for the newbies and trainees it will be a handy tool for infrequent users, and may benefit the experienced users as well since it provides scripts for customizing every detail of boxplots.
Long-wave Absorption of Few-Hole Gas in Prolate Ellipsoidal Ge/Si Quantum Dot: Implementation of Analytically Solvable Moshinsky Model
Authors: Hayrapetyan David B. , Kazaryan Eduard M. , Mkrtchyan Mher A. , Sarkisyan Hayk A.
Published: September 2020

In this paper, the behavior of a heavy hole gas in a strongly prolate ellipsoidal Ge/Si quantum dot has been investigated. Due to the specific geometry of the quantum dot, the interaction between holes is considered one-dimensional. Based on the adiabatic approximation, it is shown that in the z-direction, hole gas is localized in a one-dimensional parabolic well. By modeling the potential of pair interaction between holes in the framework of oscillatory law, the problem is reduced to a one-dimensional, analytically solvable Moshinsky model. The exact energy spectrum of the few-hole gas has been calculated. A detailed analysis of the energy spectrum is presented. The character of long-wave transitions between the center-of-mass levels of the system has been obtained when Kohn theorem is realized.
Modification of V2O5-WO3/TiO2 Catalyst by Loading of MnOx for Enhanced Low-Temperature NH3-SCR Performance
Authors: Zhang Xianlong , Diao Qinchao , Hu Xiaorui , Wu Xueping , Xiao Kesong , Wang Junwei
Published: September 2020

V2O5-WO3/TiO2 as a commercial selective catalytic reduction (SCR) catalyst usually used at middle-high temperatures was modified by loading of MnOx for the purpose of enhancing its performance at lower temperatures. Manganese oxides were loaded onto V-W/Ti monolith by the methods of impregnation (I), precipitation (P), and in-situ growth (S), respectively. SCR activity of each modified catalyst was investigated at temperatures in the range of 100–340 °C. Catalysts were characterized by specific surface area and pore size determination (BET), X-ray diffraction (XRD), temperature programmed reduction (TPR), etc. Results show that the loading of MnOx remarkably enhanced the SCR activity at a temperature lower than 280 °C. The catalyst prepared by the in-situ growth method was found to be most active for SCR.
Low Working Temperature of ZnO-MoS2 Nanocomposites for Delaying Aging with Good Acetylene Gas-Sensing Properties
Authors: Wang Sijie , Chen Weigen , Li Jian , Song Zihao , Zhang He , Zeng Wen
Published: September 2020

The long-term stability and the extension of the use time of gas sensors are one of the current concerns. Lowering the working temperature is one of the most effective methods to delay aging. In this paper, pure MoS2 and ZnO-MoS2 nanocomposites were successfully prepared by the hydrothermal method, and the morphological characteristics were featured by scanning electron microscopy (SEM), X-ray diffraction (XRD), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). Pure MoS2 and ZnO-MoS2 nanocomposites, as a comparison, were used to study the aging characteristic. The sensing properties of the fabricated gas sensors with an optimal molar ratio ZnO-MoS2 (Zn:Mo = 1:2) were recorded, and the results exhibit a high gas-sensing response and good repeatability to the acetylene detection. The working temperature was significantly lower than for pure MoS2. After aging for 40 days, all the gas-sensing response was relatively attenuated, and pure MoS2 exhibits a faster decay rate and lower gas-sensing response than nanocomposites. The better gas-sensing characteristic of nanocomposites after aging was possibly attributed to the active interaction between ZnO and MoS2.
Physiologic Effects of Exogenous Dextrose in Murine Klebsiella pneumoniae Sepsis Vary by Route of Provision
Authors: Chuan Byron , Guo Lanping , Cooper Bryce , Rawal Sagar , Gallego-Martin Teresa , Zhang Yingze , McVerry Bryan J. , O’Donnell Christopher P. , Shah Faraaz Ali
Published: September 2020

Sepsis is characterized by a dysregulated immune response to infection. Nutrition is important in the care of septic patients, but the effects of specific nutrients on inflammation in sepsis are not well defined. Our prior work has shown benefits from early enteral dextrose infusion in a preclinical endotoxemia model of sepsis. In the current study, we extend our initial work to examine the effects of dextrose infusions, varying by route of administration, on inflammation and glycemic control in a more clinically relevant and translational model of Klebsiella pneumoniae (KP) bacteremia. Ten-week old C57BL6/J male mice (n = 31) underwent the implantation of indwelling vascular catheters, followed by inoculation with oropharyngeal KP. The mice were randomized 24 h after inoculation to (1) intravenous (IV) dextrose, (2) enteral dextrose, or (3) enteral saline (control) to study the effects on systemic inflammation, hemodynamics, and glycemic control. At 72 h, 77% of the control mice died, whereas IV dextrose induced 100% mortality, associated with increased inflammation, hyperglycemia, and hypotension. Enteral dextrose reduced mortality to 27%, promoted euglycemia, and reduced inflammation compared to IV dextrose. We conclude, in a bacteremic model of sepsis, that enteral (but not IV) dextrose administration is protective, suggesting that the route of nutrient support influences inflammation in sepsis.
An Exploratory Analysis of Changes in Mental Wellbeing Following Curcumin and Fish Oil Supplementation in Middle-Aged and Older Adults
Authors: Kuszewski Julia C. , Howe Peter R. C. , Wong Rachel H. X.
Published: September 2020

Curcumin has previously been shown to enhance mood in non-depressed older adults. However, observed benefits were limited to short-term supplementation (4 weeks). In a 16 week randomized, double-blind, placebo-controlled, 2 × 2 factorial design trial, we supplemented overweight or obese non-depressed adults (50–80 years) with curcumin (160 mg/day), fish oil (2000 mg docosahexaenoic acid +400 mg eicosapentaenoic acid/day), or a combination of both. Secondary outcomes included mental wellbeing measures (mood states and subjective memory complaints (SMCs)) and quality of life (QoL). Furthermore, plasma apolipoprotein E4 (APOE4) was measured to determine whether APOE4 status influences responses to fish oil. Curcumin improved vigour (p = 0.044) compared to placebo and reduced SMCs compared to no curcumin treatment (p = 0.038). Fish oil did not affect any mood states, SMCs or QoL; however, responses to fish oil were affected by APOE4 status. In APOE4 non-carriers, fish oil increased vigour (p = 0.030) and reduced total mood disturbances (p = 0.048) compared to placebo. Improvements in mental wellbeing were correlated with increased QoL. Combining curcumin with fish oil did not result in additive effects. This exploratory analysis indicates that regular supplementation with either curcumin or fish oil (limited to APOE4 non-carriers) has the potential to improve some aspects of mental wellbeing in association with better QoL.