bluuebunny/crossref_metadata_2025 huggingface dataset:
Biological Factors behind Melanoma Response to Immune Checkpoint Inhibitors
Authors: Olbryt Magdalena , Rajczykowski Marcin , Widłak Wiesława
Published: June 2020

Modern immunotherapy together with targeted therapy has revolutionized the treatment of advanced melanoma. Inhibition of immune checkpoints significantly improved the median overall survival and gave hope to many melanoma patients. However, this treatment has three serious drawbacks: high cost, serious side effects, and an effectiveness limited only to approximately 50% of patients. Some patients do not derive any or short-term benefit from this treatment due to primary or secondary resistance. The response to immunotherapy depends on many factors that fall into three main categories: those associated with melanoma cells, those linked to a tumor and its microenvironment, and those classified as individual ontogenic and physiological features of the patient. The first category comprises expression of PD-L1 and HLA proteins on melanoma cells as well as genetic/genomic metrics such as mutational load, (de)activation of specific signaling pathways and epigenetic factors. The second category is the inflammatory status of the tumor: “hot” versus “cold” (i.e., high versus low infiltration of immune cells). The third category comprises metabolome and single nucleotide polymorphisms of specific genes. Here we present up-to-date data on those biological factors influencing melanoma response to immunotherapy with a special focus on signaling pathways regulating the complex process of anti-tumor immune response. We also discuss their potential predictive capacity.
The Emerging Role of Exosomes in Diagnosis, Prognosis, and Therapy in Head and Neck Cancer
Authors: Hofmann Linda , Ludwig Sonja , Vahl Julius M. , Brunner Cornelia , Hoffmann Thomas K. , Theodoraki Marie-Nicole
Published: June 2020

Exosomes, the smallest group of extracellular vesicles, carry proteins, miRNA, mRNA, DNA, and lipids, which they efficiently deliver to recipient cells, generating a communication network. Exosomes strongly contribute to the immune suppressive tumor microenvironment of head and neck squamous cell carcinomas (HNSCC). Isolation of exosomes from HNSCC cell culture or patient’s plasma allows for analyzing their molecular cargo and functional role in immune suppression and tumor progression. Immune affinity-based separation of different exosome subsets, such as tumor-derived or T cell-derived exosomes, from patient’s plasma simultaneously informs about tumor status and immune dysfunction. In this review, we discuss the recent understanding of how exosomes behave in the HNSCC tumor microenvironment and why they are promising liquid biomarkers for diagnosis, prognosis, and therapy in HNSCC.
Reg3α and Reg3β Expressions Followed by JAK2/STAT3 Activation Play a Pivotal Role in the Acceleration of Liver Hypertrophy in a Rat ALPPS Model
Authors: Otsuka Naohiko , Yoshioka Masato , Abe Yuki , Nakagawa Yasuhiko , Uchinami Hiroshi , Yamamoto Yuzo
Published: June 2020

To explore the underlying mechanism of rapid liver hypertrophy by liver partition in associating liver partition and portal vein ligation for staged hepatectomy (ALPPS), liver partition at different sites was investigated. Increased inflammatory cytokines owing to the liver partition have been reportedly responsible. If this were true, rapid liver hypertrophy should be achieved regardless of where the liver was split. A male Sprague-Dawley rat model was created, in which a liver split was placed inside the portal vein ligated lobe (PiLL), in addition to the ALPPS and portal vein ligation (PVL) models. Liver regeneration rate, inflammatory cytokine levels, activation status of the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway and expressions of regenerating islet-derived (Reg)3α and Reg3β were investigated. The liver regeneration rate was significantly higher in the ALPPS group than in the PiLL group, whereas inflammatory cytokine levels were nearly equal. Additional volume increase in ALPPS group over PVL and PiLL groups was JAK2/STAT3-dependent. Reg3α and Reg3β expressions were observed only in the ALPPS group. An increase in inflammatory cytokines was not enough to describe the mechanism of rapid liver hypertrophy in ALPPS. Expressions of Reg3α and Reg3β could play an important role in conjunction with an activation of the JAK2/STAT3 pathway.
AtHB7/12 Regulate Root Growth in Response to Aluminum Stress
Authors: Liu Yang , Xu Jiameng , Guo Siyi , Yuan Xianzheng , Zhao Shan , Tian Huiyu , Dai Shaojun , Kong Xiangpei , Ding Zhaojun
Published: June 2020

Aluminum (Al) stress is a major limiting factor for plant growth and crop production in acid soils. At present, only a few transcription factors involved in the regulation of Al resistance have been characterized. Here, we used reversed genetic approach through phenotype analysis of overexpressors and mutants to demonstrate that AtHB7 and AtHB12, two HD-Zip I transcription factors, participate in Al resistance. In response to Al stress, AtHB7 and AtHB12 displayed different dynamic expression patterns. Although both AtHB7 and AtHB12 positively regulate root growth in the absence of Al stress, our results showed that AtHB7 antagonizes with AtHB12 to control root growth in response to Al stress. The athb7/12 double mutant displayed a wild-type phenotype under Al stress. Consistently, our physiological analysis showed that AtHB7 and AtHB12 oppositely regulate the capacity of cell wall to bind Al. Yeast two hybrid assays showed that AtHB7 and AtHB12 could form homo-dimers and hetero-dimers in vitro, suggesting the interaction between AtHB7 and AtHB12 in the regulation of root growth. The conclusion was that AtHB7 and AtHB12 oppositely regulate Al resistance by affecting Al accumulation in root cell wall.
Specificity in Ubiquitination Triggered by Virus Infection
Authors: Gu Haidong , Jan Fada Behdokht
Published: June 2020

Ubiquitination is a prominent posttranslational modification, in which the ubiquitin moiety is covalently attached to a target protein to influence protein stability, interaction partner and biological function. All seven lysine residues of ubiquitin, along with the N-terminal methionine, can each serve as a substrate for further ubiquitination, which effectuates a diverse combination of mono- or poly-ubiquitinated proteins with linear or branched ubiquitin chains. The intricately composed ubiquitin codes are then recognized by a large variety of ubiquitin binding domain (UBD)-containing proteins to participate in the regulation of various pathways to modulate the cell behavior. Viruses, as obligate parasites, involve many aspects of the cell pathways to overcome host defenses and subjugate cellular machineries. In the virus-host interactions, both the virus and the host tap into the rich source of versatile ubiquitination code in order to compete, combat, and co-evolve. Here, we review the recent literature to discuss the role of ubiquitin system as the infection progresses in virus life cycle and the importance of ubiquitin specificity in the regulation of virus-host relation.
Reactive Glia Inflammatory Signaling Pathways and Epilepsy
Authors: Sanz Pascual , Garcia-Gimeno Maria Adelaida
Published: June 2020

Neuroinflammation and epilepsy are interconnected. Brain inflammation promotes neuronal hyper-excitability and seizures, and dysregulation in the glia immune-inflammatory function is a common factor that predisposes or contributes to the generation of seizures. At the same time, acute seizures upregulate the production of pro-inflammatory cytokines in microglia and astrocytes, triggering a downstream cascade of inflammatory mediators. Therefore, epileptic seizures and inflammatory mediators form a vicious positive feedback loop, reinforcing each other. In this work, we have reviewed the main glial signaling pathways involved in neuroinflammation, how they are affected in epileptic conditions, and the therapeutic opportunities they offer to prevent these disorders.
DHA Modulates Immune Response and Mitochondrial Function of Atlantic Salmon Adipocytes after LPS Treatment
Authors: Bou Marta , Torgersen Jacob Seilø , Østbye Tone-Kari Knutsdatter , Ruyter Bente , Wang Xinxia , Škugor Stanko , Kristiansen Inger Øien , Todorčević Marijana
Published: June 2020

Adipocytes play a central role in overall energy homeostasis and are important contributors to the immune system. Fatty acids (FAs) act as signaling molecules capable to modulate adipocyte metabolism and functions. To identify the effects of two commonly used FAs in Atlantic salmon diets, primary adipocytes were cultured in the presence of oleic (OA) or docosahexaenoic (DHA) acid. DHA decreased adipocyte lipid droplet number and area compared to OA. The increase in lipid load in OA treated adipocytes was paralleled by an increase in iNOS activity and mitochondrial SOD2-GFP activity, which was probably directed to counteract increase in oxidative stress. Under lipopolysaccharide (LPS)-induced inflammation, DHA had a greater anti-inflammatory effect than OA, as evidenced by the higher SOD2 activity and the transcriptional regulation of antioxidant enzymes and pro- and anti-inflammatory markers. In addition, DHA maintained a healthy mitochondrial structure under induced inflammation while OA led to elongated mitochondria with a thin thread like structures in adipocytes exposed to LPS. Overall, DHA possess anti-inflammatory properties and protects Atlantic salmon against oxidative stress and limits lipid deposition. Furthermore, DHA plays a key role in protecting mitochondria shape and function.
Editorial for Special Issue “Heavy Metals Accumulation, Toxicity, and Detoxification in Plants”
Authors: Aprile Alessio , De Bellis Luigi
Published: June 2020

“Heavy metals” is a collective term widely applied for the group of metals and metalloids with an atomic density above 4 g/cm3 [...]
The Role of Pref-1 during Adipogenic Differentiation: An Overview of Suggested Mechanisms
Authors: da Silva Carina , Durandt Chrisna , Kallmeyer Karlien , Ambele Melvin A. , Pepper Michael S.
Published: June 2020

Obesity contributes significantly to the global health burden. A better understanding of adipogenesis, the process of fat formation, may lead to the discovery of novel treatment strategies. However, it is of concern that the regulation of adipocyte differentiation has predominantly been studied using the murine 3T3-L1 preadipocyte cell line and murine experimental animal models. Translation of these findings to the human setting requires confirmation using experimental models of human origin. The ability of mesenchymal stromal/stem cells (MSCs) to differentiate into adipocytes is an attractive model to study adipogenesis in vitro. Differences in the ability of MSCs isolated from different sources to undergo adipogenic differentiation, may be useful in investigating elements responsible for regulating adipogenic differentiation potential. Genes involved may be divided into three broad categories: early, intermediate and late-stage regulators. Preadipocyte factor-1 (Pref-1) is an early negative regulator of adipogenic differentiation. In this review, we briefly discuss the adipogenic differentiation potential of MSCs derived from two different sources, namely adipose-derived stromal/stem cells (ASCs) and Wharton’s Jelly derived stromal/stem cells (WJSCs). We then discuss the function and suggested mechanisms of action of Pref-1 in regulating adipogenesis, as well as current findings regarding Pref-1’s role in human adipogenesis.
N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice
Authors: Chaves Cayuela Natalie , Kiyomi Koike Marcia , Jacysyn Jacqueline , Rasslan Roberto , Azevedo Cerqueira Anderson , Pereira Costa Soraia , Picanço Diniz-Júnior José , Massazo Utiyama Edivaldo , Frasson de Souza Montero Edna
Published: June 2020

N-acetylcysteine (NAC) is a pharmacological alternative with great potential for reducing the deleterious effects of surgical procedures on patients with steatohepatitis. We evaluated the effect of NAC on hepatic ischemia/reperfusion (I/R) injury in C57BL/6J mice, 8 weeks-old, weighing 25–30 g, with steatohepatitis induced by a methionine- and choline-deficient (MCD) diet. Groups: MCD group (steatohepatitis), MCD-I/R group (steatohepatitis plus 30 min of 70% liver ischemia and 24 h of reperfusion), MCD-I/R+NAC group (same as MCD-I/R group plus 150 mg/kg NAC 15 min before ischemia), and control group (normal AIN-93M diet). Liver enzymes and histopathology; nitrite and TBARS (thiobarbituric acid reactive substances) levels; pro-inflammatory cytokines; antioxidants enzymes; Nrf2 (nuclear factor erythroid-2-related factor 2) expression; and apoptosis were evaluated. In the group treated with NAC, reductions in inflammatory infiltration; AST (aspartate aminotransferase), nitrite, and TBARS levels; GPx (gutathione peroxidase) activity; cytokines synthesis; and number of apoptotic cells were observed while the GR (glutathione reductase) activity was increased. No differences were observed in Nfr2 expression or in SOD (superoxide dismutase), CAT (catalase), and GST (glutathione S-transferase) activities. Thus, it may be concluded that NAC exerts beneficial effects on mice livers with steatohepatitis submitted to I/R by reducing oxidative stress, inflammatory response, and cell death.
Advances in Plant Boron
Authors: González-Fontes Agustín , Fujiwara Toru
Published: June 2020

Although very recently, David H [...]
The Cellular and Molecular Determinants of Naphthoquinone-Dependent Activation of the Aryl Hydrocarbon Receptor
Authors: Faber Samantha C. , Giani Tagliabue Sara , Bonati Laura , Denison Michael S.
Published: June 2020

1,2-naphthoquinone (1,2-NQ) and 1,4-naphthoquinone (1,4-NQ) are clinically promising biologically active chemicals that have been shown to stimulate the aryl hydrocarbon receptor (AhR) signaling pathway, but whether they are direct or indirect ligands or activate the AhR in a ligand-independent manner is unknown. Given the structural diversity of AhR ligands, multiple mechanisms of AhR activation of gene expression, and species differences in AhR ligand binding and response, we examined the ability of 1,2-NQ and 1,4-NQ to bind to and activate the mouse and human AhRs using a series of in vitro AhR-specific bioassays and in silico modeling techniques. Both NQs induced AhR-dependent gene expression in mouse and human hepatoma cells, but were more potent and efficacious in human cells. 1,2-NQ and 1,4-NQ stimulated AhR transformation and DNA binding in vitro and was inhibited by AhR antagonists. Ligand binding analysis confirmed the ability of 1,2-NQ and 1,4-NQ to competitively bind to the AhR ligand binding cavity and the molecular determinants for interactions were predicted by molecular modeling methods. NQs were shown to bind distinctly differently from that of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and differences were also observed between species. Mutation of amino acid residues (F289, M334, and M342) involved in critical NQ:AhR binding interactions, decreased NQ- and AhR-dependent gene expression, consistent with a role for these residues in binding and activation of the AhR by NQs. These studies provide insights into the molecular mechanism of action of NQs and contribute to the development of emerging NQ-based therapeutics.
Ruthenium Dendrimers against Human Lymphoblastic Leukemia 1301 Cells
Authors: Michlewska Sylwia , Ionov Maksim , Szwed Aleksandra , Rogalska Aneta , Sanz del Olmo Natalia , Ortega Paula , Denel Marta , Jacenik Damian , Shcharbin Dzmitry , de la Mata Francisco Javier , Bryszewska Maria
Published: June 2020

Ruthenium atoms located in the surfaces of carbosilane dendrimers markedly increase their anti-tumor properties. Carbosilane dendrimers have been widely studied as carriers of drugs and genes owing to such characteristic features as monodispersity, stability, and multivalence. The presence of ruthenium in the dendrimer structure enhances their successful use in anti-cancer therapy. In this paper, the activity of dendrimers of generation 1 and 2 against 1301 cells was evaluated using Transmission Electron Microscopy, comet assay and Real Time PCR techniques. Additionally, the level of reactive oxygen species (ROS) and changes of mitochondrial potential values were assessed. The results of the present study show that ruthenium dendrimers significantly decrease the viability of leukemia cells (1301) but show low toxicity to non-cancer cells (peripheral blood mononuclear cells—PBMCs). The in vitro test results indicate that the dendrimers injure the 1301 leukemia cells via the apoptosis pathway.
Position Control of Cable-Driven Robotic Soft Arm Based on Deep Reinforcement Learning
Authors: Wu Qiuxuan , Gu Yueqin , Li Yancheng , Zhang Botao , Chepinskiy Sergey A. , Wang Jian , Zhilenkov Anton A. , Krasnov Aleksandr Y. , Chernyi Sergei
Published: June 2020

The cable-driven soft arm is mostly made of soft material; it is difficult to control because of the material characteristics, so the traditional robot arm modeling and control methods cannot be directly applied to the soft robot arm. In this paper, we combine the data-driven modeling method with the reinforcement learning control method to realize the position control task of robotic soft arm, the method of control strategy based on deep Q learning. In order to solve slow convergence and unstable effect in the process of simulation and migration when deep reinforcement learning is applied to the actual robot control task, a control strategy learning method is designed, which is based on the experimental data, to establish a simulation environment for control strategy training, and then applied to the real environment. Finally, it is proved by experiment that the method can effectively complete the control of the soft robot arm, which has better robustness than the traditional method.
Examining the Relationship between Semiquantitative Methods Analysing Concentration-Time and Enhancement-Time Curves from Dynamic-Contrast Enhanced Magnetic Resonance Imaging and Cerebrovascular Dysfunction in Small Vessel Disease
Authors: Bernal Jose , Valdés-Hernández María , Escudero Javier , Sakka Eleni , Armitage Paul A. , Makin Stephen , Touyz Rhian M. , Wardlaw Joanna M.
Published: June 2020

Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) can be used to examine the distribution of an intravenous contrast agent within the brain. Computational methods have been devised to analyse the contrast uptake/washout over time as reflections of cerebrovascular dysfunction. However, there have been few direct comparisons of their relative strengths and weaknesses. In this paper, we compare five semiquantitative methods comprising the slope and area under the enhancement-time curve, the slope and area under the concentration-time curve ( S l o p e C o n and A U C C o n ), and changes in the power spectrum over time. We studied them in cerebrospinal fluid, normal tissues, stroke lesions, and white matter hyperintensities (WMH) using DCE-MRI scans from a cohort of patients with small vessel disease (SVD) who presented mild stroke. The total SVD score was associated with A U C C o n in WMH ( p < 0.05 ), but not with the other four methods. In WMH, we found higher A U C C o n was associated with younger age ( p < 0.001 ) and fewer WMH ( p < 0.001 ), whereas S l o p e C o n increased with younger age ( p > 0.05 ) and WMH burden ( p > 0.05 ). Our results show the potential of different measures extracted from concentration-time curves extracted from the same DCE examination to demonstrate cerebrovascular dysfunction better than those extracted from enhancement-time curves.
Using Autonomous Underwater Vehicles for Diver Tracking and Navigation Aiding
Authors: Nađ Đula , Mandić Filip , Mišković Nikola
Published: June 2020

SCUBA diving activities are classified as high-risk due to the dangerous environment, dependency on technical equipment that ensures life support, reduced underwater navigation and communication capabilities all of which compromise diver safety. While autonomous underwater vehicles (AUVs) have become irreplaceable tools for seabed exploration, monitoring, and mapping in various applications, they still lack the higher cognitive capabilities offered by a human diver. The research presented in this paper was carried out under the EU FP7 “CADDY—Cognitive Autonomous Diving Buddy”. It aims to take advantage of both human diver and AUV complementary traits by making their synergy a potential solution for mitigation of state of the art diving challenges. The AUV increases diver safety by constantly observing the diver, provides navigation aiding by directing the diver and offers assistance (e.g., lights, tool fetching, etc.). The control algorithms proposed in the paper provide a foundation for implementing these services. These algorithms use measurements from stereo-camera, sonar and ultra-short baseline acoustic localization to ensure the vehicle constantly follows and observes the diver. Additionally, the vehicle maintains a relative formation with the diver to allow observation from multiple viewpoints and to aid underwater navigation by pointing towards the next point of interest. Performance of the proposed algorithms is evaluated using results from pool experiments.
Integrating Wind Turbines and Fish Farms: An Evaluation of Potential Risks to Marine and Coastal Bird Species
Authors: Benjamins Steven , Masden Elizabeth , Collu Maurizio
Published: June 2020

Expansion of marine aquaculture into more remote areas will likely accelerate over the next decade. Integrating Marine Renewable Energy (MRE) generation technologies (e.g., wind turbines) into remote, off-grid aquaculture sites will reduce reliance on fossil fuels by allowing localised low-carbon power generation, but may also result in novel environmental pressures. In this study, we undertook a thought experiment to assess the potential for increased collision risks to local marine and coastal bird species of integrating small wind turbines (4 units; combined capacity of 200 MWh) into a generalised marine fish farm in western Scotland (UK). Potential risks to bird species were assessed using a bespoke Sensitivity Index (SI) based on 12 factors, including population size, adult survival rate, UK conservation status, flight manoeuvrability, nocturnal flight activity, habitat preference, sensitivity to wind farms, attraction to fish farms for feeding and/or resting, and attraction to other marine anthropogenic structures/activities. SI scores varied substantially between species, but large gulls (Larus sp.) and European shag (Phalacrocorax aristotelis) were expected to be at the greatest potential risk. The general lack of information on interactions between birds and fish farms represented a significant knowledge gap, and greater focus on these interactions is needed to improve future risk assessments.
Wave-Generated Current: A Second-Order Formulation
Authors: Bratland Anne Katrine
Published: June 2020

In Stokes’ wave theory, wave numbers are corrected in the third order solution. A change in wave number is also associated with a change in current velocity. Here, it will be argued that the current is the reason for the wave number correction, and that wave-generated current at the mean free surface in infinite depth equals half the Stokes drift. To demonstrate the validity of this second-order formulation, comparisons to computational fluid dynamics (CFD) results are shown; to indicate its effect on wave loads on structures, model tests and analyses are compared.
Metadiscourse, Cohesion, and Engagement in L2 Written Discourse
Authors: Liao Jianling
Published: June 2020

The current study examines how L2 Chinese writers at different proficiencies employed various metadiscourse devices to shape their written descriptive discourse and also whether various metadiscourse features may distinguish levels of writing proficiency. The study also looks at how L2 learners’ use of metadiscourse devices is related to their linguistic performances in descriptive writing. The findings revealed differential metadiscourse use by learners at different proficiencies on local, global, and textual organizational dimensions. For instance, compared to low-proficiency writers, more proficient writers used significantly more conditional/hypothetical markers, frame markers, and engagement markers. Multiple metadiscourse features also demonstrated significant positive and negative correlations with each other, suggesting patterns of decreases and increases in the use of particular organizational features. Several metadiscourse features characteristic of more advanced writers also displayed positive relationships with linguistic features.
Study on Low-Velocity Impact Damage and Residual Strength of Reinforced Composite Skin Structure
Authors: Li Hanhua , Zhang Qiuhua , Jia Jiale , Ji Chunming , Wang Bing , Yan Shi
Published: June 2020

In order to better understand the damage tolerance of reinforced composite plates, the impact damage of the reinforced composite plates was investigated under low-velocity impact test. The experimental results show that the impact of different positions and energies causes different degrees of damage to the specimens, including but not limited to ply fracture, internal delamination of the skin, and debonding of the stiffeners and skin. After impacting, the specimens were tested in an axial compression. The results show that the ultimate bearing capacity of the specimen is also affected by different forms of impact. The impact point has the greatest influence on the specimen while it locates at the intersection of longitudinal and transverse bars. Compared with the intact specimen, the ultimate load carrying capacity was reduced by 16.83% and 44.02%, while the specimen impacted by 15 J and 30 J, respectively. The compression failure mode of the damaged specimen is mainly the breakage of the stiffeners and the delamination of the skin.
Label-Free Split Aptamer Sensor for Femtomolar Detection of Dopamine by Means of Flexible Organic Electrochemical Transistors
Authors: Liang Yuanying , Guo Ting , Zhou Lei , Offenhäusser Andreas , Mayer Dirk
Published: June 2020

The detection of chemical messenger molecules, such as neurotransmitters in nervous systems, demands high sensitivity to measure small variations, selectivity to eliminate interferences from analogues, and compliant devices to be minimally invasive to soft tissue. Here, an organic electrochemical transistor (OECT) embedded in a flexible polyimide substrate is utilized as transducer to realize a highly sensitive dopamine aptasensor. A split aptamer is tethered to a gold gate electrode and the analyte binding can be detected optionally either via an amperometric or a potentiometric transducer principle. The amperometric sensor can detect dopamine with a limit of detection of 1 μM, while the novel flexible OECT-based biosensor exhibits an ultralow detection limit down to the concentration of 0.5 fM, which is lower than all previously reported electrochemical sensors for dopamine detection. The low detection limit can be attributed to the intrinsic amplification properties of OECTs. Furthermore, a significant response to dopamine inputs among interfering analogues hallmarks the selective detection capabilities of this sensor. The high sensitivity and selectivity, as well as the flexible properties of the OECT-based aptasensor, are promising features for their integration in neuronal probes for the in vitro or in vivo detection of neurochemical signals.
Full-Field Strain Uncertainties and Residuals at the Cartilage-Bone Interface in Unstained Tissues Using Propagation-Based Phase-Contrast XCT and Digital Volume Correlation
Authors: Tozzi Gianluca , Peña Fernández Marta , Davis Sarah , Karali Aikaterina , Kao Alexander Peter , Blunn Gordon
Published: June 2020

A deeper understanding of the cartilage-bone mechanics is fundamental to unravel onset and progression of osteoarthritis, enabling better diagnosis and treatment. The aim of this study is therefore to explore the capability of X-ray computed (XCT) phase-contrast imaging in a lab-based system to enable digital volume correlation (DVC) measurements of unstained cartilage-bone plugs from healthy adult bovines. DVC strain uncertainties were computed for both articular cartilage and mineralized tissue (calcified cartilage and subchondral bone) in the specimens at increasing propagation distances, ranging from absorption up to four times (4× such effective distance. In addition, a process of dehydration and rehydration was proposed to improve feature recognition in XCT of articular cartilage and mechanical properties of this tissue during the process were assessed via micromechanical probing (indentation), which was also used to determine the effect of long X-ray exposure. Finally, full-field strain from DVC was computed to quantify residual strain distribution at the cartilage-bone interface following unconfined compression test (ex situ). It was found that enhanced gray-scale feature recognition at the cartilage-bone interface was achieved using phase-contrast, resulting in reduced DVC strain uncertainties compared to absorption. Residual strains up to ~7000 µε in the articular cartilage were transferred to subchondral bone via the calcified cartilage and micromechanics revealed the predominant effect of long phase-contrast X-ray exposure in reducing both stiffness and hardness of the articular cartilage. The results of this study will pave the way for further development and refinement of the techniques, improving XCT-based strain measurements in cartilage-bone and other soft-hard tissue interfaces.
New Power MOSFET with Beyond-1D-Limit RSP-BV Trade-Off and Superior Reverse Recovery Characteristics
Authors: Zhang Meng , Li Baikui , Wei Jin
Published: June 2020

The application of conventional power metal-oxide-semiconductor field-effect transistor (MOSFET) is limited by the famous one-dimensional “silicon limit” (1D-limit) in the trade-off relationship between specific on-resistance (RSP) and breakdown voltage (BV). In this paper, a new power MOSFET architecture is proposed to achieve a beyond-1D-limit RSP-BV trade-off. Numerical TCAD (technology computer-aided design) simulations were carried out to comparatively study the proposed MOSFET, the conventional power MOSFET, and the superjunction MOSFET. All the devices were designed with the same breakdown voltage of ~550 V. The proposed MOSFET features a deep trench between neighboring p-bodies and multiple p-islands located at the sidewall and bottom of the trench. The proposed MOSFET allows a high doping concentration in the drift region, which significantly reduces its RSP compared to the conventional power MOSFET. The multiple p-islands split the electric field into multiple peaks and help the proposed MOSFET maintain a similar breakdown voltage to the conventional power MOSFET with the same drift region thickness. Another famous device technology, the superjunction MOSFET (SJ-MOSFET), also breaks the 1D-limit. However, the SJ-MOSFET suffers a snappy reverse recovery performance, which is a notorious drawback of SJ-MOSFET and limits the range of its application. On the contrary, the proposed MOSFET presents a superior reverse recovery performance and can be used in various power switching applications where hard commutation is required.
Axial Compression Experiments and Finite Element Analysis of Basalt Fiber/Epoxy Resin Three-Dimensional Tubular Woven Composites
Authors: Zhu Liming , Zhang Huawei , Guo Jing , Wang Ying , Lyu Lihua
Published: June 2020

In order to avoid the delamination of traditional tubular composite materials and reduce its woven cost, on an ordinary loom, the three-dimensional (3D) tubular woven fabrics were woven with basalt filament tows, and then the 3D tubular woven composites were prepared with epoxy resin by a hand layup process. The wall thickness of the 3D tubular woven composite was thin, and was only 2 mm thick. Through experiments and finite element method (FEM) simulation, the axial compression properties of the material were analyzed. The results show that the material 2 mm thick has good axial compression performance, the maximum load value of the experiment is 10,578 N, and the maximum load value of the finite element simulation is 11,285 N. The error between the two is 6.68%, indicating that the experiment and simulation have a good consistency. The failure mode of the material is also analyzed through finite element method simulation in the paper, thus revealing the failure stress propagation, local stress concentration, and failure morphology of the material. It provides an effective reference for the design and application of the 3D tubular woven composite.
Investigations on the Processing of Ceramic Filled Inks for 3D InkJet Printing
Authors: Graf Dennis , Qazzazie Afnan , Hanemann Thomas
Published: June 2020

3D inkjet printing is moving from a technology of rapid prototyping to rapid manufacturing. The introduction of ultraviolet curable composites filled with functional ceramics could expand the possibilities of this technology. In this work, a simple and scalable process was investigated as a template for the production of inkjet printable functional ceramics. Pyrogenic alumina particles with an average size of 13 nm, 35 nm and 100 nm were used as fillers in an acrylate mixture. The physical coating of the ceramics with 2-[2-(2-methoxyethoxy)ethoxy] acetic acid results in a low-viscosity dispersion with a ceramic content of up to 2 vol%, Newtonian behavior and surface tension within the limits allowed for inkjet printing. The material has sufficient stability for printing tensile specimens. Tensile tests have shown that modulus of elasticity, tensile strength and toughness can be kept constant despite the light scatter caused by the particles. The final production steps could be reduced to grinding and drying of the powders, their resuspension in the organic matrix and inkjet printing. The process can be used in an industrial-scale production of materials for abrasion-resistant components with adapted tribology.
Effect of Curing Methods on Shrinkage Development in 3D-Printed Concrete
Authors: Federowicz Karol , Kaszyńska Maria , Zieliński Adam , Hoffmann Marcin
Published: June 2020

Technological developments in construction have led to an increase in the use of 3D modelling using CAD environments. The popularity of this approach has increased in tandem with developments in industry branches which use 3D printers to print concrete based printing materials in construction, as these allow freedom in shaping the dimensions of supporting elements. One of the biggest challenges for researchers working on this highly innovative technology is that of cement material shrinkage. This article presents the findings of research on an original method of measuring deformations caused by shrinkage in 3D-printed concrete elements. It also discusses the results of tests on base mixes, as well as comparisons between the influence of internal and external curing methods on the development of deformations and their final outcomes. Furthermore, the article discusses differences between deformations formed after seven days of hardening without curing, with those which occur when two common, traditional concrete curing methods are used: foil insulation and shrinkage reducing admixtures. In addition, the article examines the effects of internal curing on the 1, 7, 14, 21 and 28 day mechanical properties of concrete, in accordance with EN 196-1 and EN 12390-2. Studies have shown that the optimal amount of shrinkage reducing admixtures is 4% (in relation to the mass of cement), resulting in a reduction in total shrinkage of 23%. The use of a shrinkage reducing admixture in 3D-printed concrete does not affect their strength after 28 days, but slows the strength development during the first 7 days.
Preparation and Microstructural Characterization of a High-Cr White Cast Iron Reinforced with WC Particles
Authors: Moreira Aida B. , Ribeiro Laura M. M. , Lacerda Pedro , Sousa Ricardo O. , Pinto Ana M. P. , Vieira Manuel F.
Published: June 2020

High-chromium white cast iron (WCI) specimens locally reinforced with WC–metal matrix composites were produced via an ex situ technique: powder mixtures of WC and Fe cold-pressed in a pre-form were inserted in the mold cavity before pouring the base metal. The microstructure of the resulting reinforcement is a matrix of martensite (α’) and austenite (γ) with WC particles evenly distributed and (Fe,W,Cr)6C carbides that are formed from the reaction between the molten metal and the inserted pre-form. The (Fe,W,Cr)6C precipitation leads to the hypoeutectic solidification of the matrix and the final microstructure consists of martensite, formed from primary austenite during cooling and eutectic constituent with (Fe,Cr)7C3 and (Fe,W,Cr)6C carbides. The presence of a reaction zone with 200 µm of thickness, between the base metal and the composite should guarantee a strong bonding between these two zones.
Spark Plasma Sintering of Copper Matrix Composites Reinforced with TiB2 Particles
Authors: Pellizzari Massimo , Cipolloni Giulia
Published: June 2020

The aim of this study is to fabricate a Cu-0.5wt%TiB2 composite by mechanical alloying (MA) and spark plasma sintering (SPS). Increasing the milling time, the powders are subjected firstly to a severe flattening process and then to intense welding, which promotes the refinement of TiB2 particles, their uniform dispersion in the metal matrix, and the adhesion between the two constituents. Sintered metal matrix composites (MMC) exhibit density values between 99 and 96%, which are generally decreased by increasing milling time in view of the stronger strain hardening. On the other side, the hardness increases with milling time due to the refinement of TiB2 particles and their improved distribution. The hardness of MMC is three times higher (225 HV0.05) than the starting hardness of atomized copper (90 HV0.05). Tensile tests show a loss of ductility, but ultimate tensile strength has been increased from 276 MPa of atomized copper to 489 MPa of MMC milled for 240 min. The thermal conductivity of MMC is comparable to that of atomized copper (300 W/mK), i.e., much higher than that of the commercial Cu-Be alloy (Uddeholm Moldmax HH, 106 W/mK) typically used for tooling applications.
Comparison of the Influence of 45S5 and Cu-Containing 45S5 Bioactive Glass (BG) on the Biological Properties of Novel Polyhydroxyalkanoate (PHA)/BG Composites
Authors: Schuhladen Katharina , Lukasiewicz Barbara , Basnett Pooja , Roy Ipsita , Boccaccini Aldo R.
Published: June 2020

Polyhydroxyalkanoates (PHAs), due to their biodegradable and biocompatible nature and their ability to be formed in complex structures, are excellent candidates for fabricating scaffolds used in tissue engineering. By introducing inorganic compounds, such as bioactive glasses (BGs), the bioactive properties of PHAs can be further improved. In addition to their outstanding bioactivity, BGs can be additionally doped with biological ions, which in turn extend the functionality of the BG-PHA composite. Here, different PHAs were combined with 45S5 BG, which was additionally doped with copper in order to introduce antibacterial and angiogenic properties. The resulting composite was used to produce scaffolds by the salt leaching technique. By performing indirect cell biology tests using stromal cells, a dose-depending effect of the dissolution products released from the BG-PHA scaffolds could be found. In low concentrations, no toxic effect was found. Moreover, in higher concentrations, a minor reduction of cell viability combined with a major increase in VEGF release was measured. This result indicates that the fabricated composite scaffolds are suitable candidates for applications in soft and hard tissue engineering. However, more in-depth studies are necessary to fully understand the release kinetics and the resulting long-term effects of the BG-PHA composites.
Cu/Ce-co-Doped Silica Glass as Radioluminescent Material for Ionizing Radiation Dosimetry
Authors: Bahout Jessica , Ouerdane Youcef , El Hamzaoui Hicham , Bouwmans Géraud , Bouazaoui Mohamed , Cassez Andy , Baudelle Karen , Habert Rémi , Morana Adriana , Boukenter Aziz , Girard Sylvain , Capoen Bruno
Published: June 2020

Optically activated glasses are essential to the development of new radiation detection systems. In this study, a bulk glassy rod co-doped with Cu and Ce ions, was prepared via the sol-gel technique and was drawn at about 2000 °C into a cylindrical capillary rod to evaluate its optical and radioluminescence properties. The sample showed optical absorption and photoluminescence (PL) bands attributed to Cu+ and Ce3+ ions. The presence of these two ions inside the host silica glass matrix was also confirmed using PL kinetics measurements. The X-ray dose rate was remotely monitored via the radioluminescence (RL) signal emitted by the Cu/Ce scintillating sensor. In order to transport the optical signal from the irradiation zone to the detection located in the instrumentation zone, an optical transport fiber was spliced to the sample under test. This RL signal exhibited a linear behavior regarding the dose rate in the range at least between 1.1 mGy(SiO2)/s and 34 Gy(SiO2)/s. In addition, a spectroscopic analysis of this RL signal at different dose rates revealed that the same energy levels attributed to Cu+ and Ce3+ ions are involved in both the RL mechanism and the PL phenomenon. Moreover, integrated intensities of the RL sub-bands related to both Cu+ and Ce3+ ions depend linearly on the dose rate at least in the investigated range from 102 mGy(SiO2)/s up to 4725 mGy(SiO2)/s. The presence of Ce3+ ions also reduces the formation of HC1 color centers after X-ray irradiation.
Comparative Fracture Properties of Four Fibre Reinforced High Performance Cementitious Composites
Authors: Smarzewski Piotr
Published: June 2020

This study investigates the fracture properties of high performance cementitious composites (HPCC) with four different types of fibres and with volume fraction content 3%. The four fibres are steel hooked end (S), polypropylene crimped (PP), basalt chopped (B), and glass (G) fibres. The tests were carried out in accordance with the RILEM recommendations. In order to examine the fresh properties of HPCC the slump flow tests were performed. Twelve fibre reinforced HPCC beam specimens with notch were cast and tested using central point loading experiments. In addition, experimental tests of the compressive strength and splitting tensile strength were carried out. The test results made it possible to obtain representative fracture parameters, such as the equivalent strengths, residual strengths, and fracture energy of fibre reinforced HPCC. The S fibre specimens showed the best performance in terms of workability, compressive strength, tensile splitting strength, and fracture energy at large deflection. On the other hand, G fibre specimens exhibited the best performance in terms of flexural strength, equivalent flexural strength at higher deflection, and residual flexural strength at lower deflection. In terms of equivalent flexural strength at lower deflection and residual flexural strength at higher deflection, basalt fibre specimens performed the best. On the contrary, polypropylene fibre reinforced beam specimens revealed the highest deflection capacity.
3D-Printed Sievenpiper Metasurface Using Conductive Filaments
Authors: Stuardo Pablo , Pizarro Francisco , Rajo-Iglesias Eva
Published: June 2020

This article presents the design, construction and measurement of different 3D-printed Sievenpiper metasurfaces. The structures were printed using a conductive filament combined with regular polylactic acid PLA. Measurement shows a good agreement on the electromagnetic behaviour of the stop-bands generated by the fully 3D-printed metasurface and the simulated ideal cases, but with higher transmission losses due to the characteristics of the conductive filament.
Equivalent Pore Channel Model for Fluid Flow in Rock Based on Microscale X-ray CT Imaging
Authors: Choi Chae-Soon , Lee Yong-Ki , Song Jae-Joon
Published: June 2020

Pore-scale modeling with a reconstructed rock microstructure has become a dominant technique for fluid flow characterization in rock thanks to technological improvements in X-ray computed tomography (CT) imaging. A new method for the construction of a pore channel model from micro-CT image analysis is suggested to improve computational efficiency by simplifying a highly complex pore structure. Ternary segmentation was applied through matching a pore volume experimentally measured by mercury intrusion porosimetry with a CT image voxel volume to distinguish regions denoted as “apparent” and “indistinct” pores. The developed pore channel model, with distinct domains of different pore phases, captures the pore shape dependence of flow in two dimensions and a tortuous flow path in three dimensions. All factors determining these geometric characteristics were identified by CT image analysis. Computation of an interaction flow regime with apparent and indistinct pore domains was conducted using both the Stokes and Brinkman equations. The coupling was successfully simulated and evaluated against the experimental results of permeability derived from Darcy’s law. Reasonable agreement was found between the permeability derived from the pore channel model and that estimated experimentally. However, the model is still incapable of accurate flow modeling in very low-permeability rock. Direct numerical simulation in a computational domain with a complex pore space was also performed to compare its accuracy and efficiency with the pore channel model. Both schemes achieved reasonable results, but the pore channel model was more computationally efficient.
Research on Recommendation Method of Product Design Scheme Based on Multi-Way Tree and Learning-to-Rank
Authors: Chen Boyang , Hu Xiaobing , Huo Yunliang , Deng Xi
Published: June 2020

A product is composed of several components, and the number, type, and combination of components plays a crucial role in the process of product design. It is difficult to get an optimized scheme in a short time. In order to improve the efficiency of product design, a product design scheme recommendation algorithm based on multi-way tree and learning-to-rank is proposed. Firstly, the product solution model, whose nodes are obtained by mapping the product attributes, is generated according to the design process, and the alternative scheme is obtained by traversing the multi-tree model. Secondly, considering users’ cognition of the importance of each product attribute, the analytic hierarchy process (AHP) is applied to assign weight to the product attribute, and then similarity to ideal solution (TOPSIS) method based on AHP is used to rank alternative solutions. Furthermore, according to users’ preference for parts’ supplier information, the learning-to-rank algorithm is used to optimize the list of alternative schemes twice. Finally, taking the design of the hoist as an example, it was verified that the proposed method had higher efficiency and better recommendation effect than the traditional parametric design method.
Feature Keypoint-Based Image Compression Technique Using a Well-Posed Nonlinear Fourth-Order PDE-Based Model
Authors: Barbu Tudor
Published: June 2020

A digital image compression framework based on nonlinear partial differential equations (PDEs) is proposed in this research article. First, a feature keypoint-based sparsification algorithm is proposed for the image coding stage. The interest keypoints corresponding to various scale-invariant image feature descriptors, such as SIFT, SURF, MSER, ORB, and BRIEF, are extracted, and the points from their neighborhoods are then used as sparse pixels and coded using a lossless encoding scheme. An effective nonlinear fourth-order PDE-based scattered data interpolation is proposed for solving the decompression task. A rigorous mathematical investigation of the considered PDE model is also performed, with the well-posedness of this model being demonstrated. It is then solved numerically by applying a consistent finite difference method-based numerical approximation algorithm that is next successfully applied in the image compression and decompression experiments, which are also discussed in this work.