bluuebunny/crossref_metadata_2025 huggingface dataset:
Highlighting the role of CD44 in cervical cancer progression: immunotherapy’s potential in inhibiting metastasis and chemoresistance
Authors: de Sousa Cayleigh , Eksteen Carla , Riedemann Johann , Engelbrecht Anna-Mart
Published: May 2024

Cervical cancer affects thousands of women globally with recurring high-risk HPV infections being at the centre of cervical pathology. Oncological treatment strategies are continually challenged by both chemoresistance and metastasis within patients. Although both work hand-in-hand, targeting their individual mechanisms could prove highly beneficial for treatment outcomes. Such targets include the metastatic-promoting stem cell marker, CD44, which is abundant in cervical cancer cells and is common to both chemoresistance and metastatic mechanisms. Seeing that many existing advanced-stage cervical cancer treatment regimes, such as platinum-based chemotherapy regimens, remain limited and are rarely curative, alternative treatment options within the field of immunology are being considered. The use of immune checkpoint inhibition therapy, which targets immune checkpoints, CTLA-4 and PD-1/PD-L1, has shown promise as an alternate standard of care for patients suffering from advanced-stage cervical cancer. Therefore, this review aims to assess whether immune checkpoint inhibition can mitigate the pathological effects of CD44-induced EMT, metastasis, and chemoresistance in cervical cancer patients.
Circularly polarized light emission and detection by chiral inorganic semiconductors
Authors: Li Zha , Li Wancai , Li Dehui , Tang Wei , Liang Huageng , Song Huaibing , Chen Chao , Gao Liang , Tang Jiang
Published: May 2024

Chiral inorganic semiconductors with high dissymmetric factor are highly desirable, but it is generally difficult to induce chiral structure in inorganic semiconductors because of their structure rigidity and symmetry. In this study, we introduced chiral ZnO film as hard template to transfer chirality to CsPbBr3 film and PbS quantum dots (QDs) for circularly polarized light (CPL) emission and detection, respectively. The prepared CsPbBr3/ZnO thin film exhibited CPL emission at 520 nm and the PbS QDs/ZnO film realized CPL detection at 780 nm, featuring high dissymmetric factor up to around 0.4. The electron transition based mechanism is responsible for chirality transfer.

Graphical Abstract
Identification and validation of a prognostic signature based on six immune-related genes for colorectal cancer
Authors: Zheng Lifeng , Xu Ziyu , Zhang Wulou , Lin Hao , Zhang Yepeng , Zhou Shu , Liu Zonghang , Gu Xi
Published: May 2024

Background

Colorectal cancer (CRC) is a prevalent malignancy with high mortality and morbidity rates. Although the significant efficacy of immunotherapy is well established, it is only beneficial for a limited number of individuals with CRC.

Methods

Differentially expressed immune-related genes (DE-IRGs) were retrieved from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), and ImmPort databases. A prognostic signature comprising DE-IRGs was developed using univariate, LASSO, and multivariate Cox regression analyses. A nomogram integrating the independent prognostic factors was also developed. CIBERSORT was used to assess immune cell infiltration (ICI). Furthermore, wound-healing, colony formation, migration, and invasion assays were performed to study the involvement of ACTG1 in CRC.

Results

A signature including six DE-IRGs was developed. The overall survival (OS) rate was accurately estimated for TCGA and GSE38832 cohorts. The risk score (RS) of the signature was an independent factor for OS. Moreover, a nomogram encompassing age, RS, and pathological T stage accurately predicted the long-term OS probability of individuals with CRC. The high-risk group had an elevated proportion of patients treated with ICIs, including native B cells, relative to the low-risk group. Additionally, ACTG1 expression was upregulated, which supported the proliferation, migration, and invasion abilities of CRC cells.

Conclusions

An immune-related prognostic signature was developed for predicting OS and for determining the immune status of individuals with CRC. The present study provides new insights into accurate immunotherapy for individuals with CRC. Moreover, ACTG1 may serve as a new immune biomarker.
A rare case report: multiple intrahepatic masses in a pediatric patient with citrin deficiency
Authors: Lin Hui , Jiang Hong , Chen Qiang , Pan Xiang , Deng Mei , Cai Xiang-Ran , Lu Yuan-Zhi , Song Yuan-Zong , Liu Jun-Cheng
Published: May 2024

Deficiency of citrin, the liver-type aspartate-glutamate carrier, arises from biallelic mutations of the gene SLC25A13. Although citrin deficiency (CD) is associated with higher risk of hepatocellular carcinoma (HCC) in adult patients, this association remains inconclusive in pediatric cases. The patient in this paper had been diagnosed to have CD by SLC25A13 analysis at the age 10 months, and then in response to dietary therapy, her prolonged jaundice and marked hepatosplenomegaly resolved gradually. However, she was referred to the hospital once again due to recurrent abdominal distention for 2 weeks at her age 4 years and 9 months, when prominently enlarged liver and spleen were palpated, along with a strikingly elevated serum alpha-fetoprotein (AFP) level of 27605 ng/mL as well as a large mass in the right liver lobe and a suspected tumor thrombus within the portal vein on enhanced computed tomography. After 4 rounds of adjuvant chemotherapy, right hepatic lobectomy and portal venous embolectomy were performed at her age 5 years and 3 months, and metastatic hepatoblastoma was confirmed by histopathological analysis. Afterwards, the patient underwent 5 additional cycles of chemotherapy and her condition remained stable for 7 months after surgery. Unfortunately, hepatoblastoma recurred in the left lobe at the age 5 years and 10 months, which progressed rapidly into liver failure, and led to death at the age 6 years and 1 month. As far as we know, this is the the first case of hepatoblastoma in a patient with CD, raising the possibility of an association between these two conditions.
Foraging behavior and the nest architecture of a high-Andean stingless bee (Hymenoptera: Apidae: Meliponini) revealed by X-ray computerized tomography
Authors: Jacome-García Jesus C. , Gonzalez Victor H. , Riaño-Jimenez Diego A. , Herrera-Motta Andres F. , Cure Jose R.
Published: May 2024

Stingless bees (Hymenoptera: Apidae: Meliponini) are key pollinators of both cultivated and wild plants in tropical and subtropical areas of the world. While most species are found in lowland to mid-elevations, a few have adapted to high elevations, and their biology remains poorly understood. We assess the foraging pattern of Parapartamona zonata (Smith) in the central Andes of Colombia (2583 m.a.s.l.) and apply computer tomography to visualize and characterize its internal nest architecture. Bees foraged for pollen and nesting materials (resin and/or mud) from sunrise (5:40 h) to sunset (17:45), even at ambient temperatures as low as 11 °C. Foraging varied significantly throughout the day and temperature and sky condition explained 47% of its variance. Differences in the nest architecture, when compared with previous records, suggest that nesting behavior might be variable. These results are discussed in the context of behavioral adaptations in this unique environmental niche.
Nondestructive and active interrogation system for special nuclear material: proof of principle and initial results
Authors: Bakr Mahmoud , Masuda Kai , Takahashi Yoshiyuki , Misawa Tsuyoshi , Yamakawa Norio , Scott Tomas
Published: May 2024

Herein, we employ the threshold energy neutron analysis (TENA) technique to introduce the world's first active interrogation system to detect special nuclear materials (SNMs), including U-235 and Pu-239. The system utilizes a DD neutron generator based on inertial electrostatic confinement (IEC) to interrogate suspicious objects. To detect secondary neutrons produced during fission reactions induced in SNMs, a tensioned metastable fluid detector (TMFD) is employed. The current status of the system's development is reported in this paper, accompanied by the results from experiments conducted to detect 10 g of highly enriched uranium (HEU). Notably, the experimental findings demonstrate a distinct difference in the count rates of measurements with and without HEU. This difference in count rates surpasses two times the standard deviation, indicating a confidence level of more than 96% for identifying the presence of HEU. The paper presents and extensively discusses the proof-of-principle experimental results, along with the system's planned trajectory.
Applications of microfluidics in biosensing
Authors: Sekhwama Masindi , Mpofu Kelvin , Sivarasu Sudesh , Mthunzi-Kufa Patience
Published: May 2024

Microfluidic devices have become a vastly popular technology, particularly because of the advantages they offer over their traditional counterparts. They have such a wide range of uses and can make complex tasks quite efficient. One area of research or work that has benefited greatly from the use of microfluidics is biosensing, where microfluidic chips are integrated into biosensor setups. There are growing numbers of applications of microfluidics in this area as researchers look for efficient ways to tackle disease diagnostics and drug discovery, which are critical in this era of recurring pandemics. In this work, the authors review the integration of microfluidic chips with biosensors, as well as microfluidic applications in biosensing, food security, molecular biology, cell diagnostics, and disease diagnostics, and look at some of the most recent research work in these areas. The work covers a wide range of applications including cellular diagnostics, life science research, agro-food processing, immunological diagnostics, molecular diagnostics, and veterinarian diagnostics. Microfluidics is a field which combines fundamental laws of physics and chemistry to solve miniaturization problems involving fluids at the nanoscale and microscale, and as such, the authors also examine some fundamental mathematical concepts in microfluidics and their applications to biosensing. Microfluidics has relatively new technologies with great potential in terms of applications.
A Historical Ethnography of the Enga Economy of Papua New Guinea
Authors: Wiessner Polly , Tumu Akii , Pupu Nitze
Published: May 2024

The question addressed in this Element is: What happens to a society when, in the absence of influence from foreign populations, constraints are released by a new crop making possible significant surplus production? We will draw on the historical traditions of 110 tribes of the Enga of Papua New Guinea recorded over a decade to document the changes that occurred in response to the potential for surplus production after the arrival of the sweet potato some 350 years prior to contact with Europeans. Economic change alone does not restructure a society nor build the social and political scaffolding for new institutions. In response to rapid change, the Enga drew on rituals that altered norms and values and resolved cultural contradictions that inhibited cooperation to bring about complexity rather than chaos. The end result was the development of one of the largest known ceremonial exchange systems prior to state formation.
Herding with the Hounds: The Game of Fifty-eight Holes in the Abşeron Peninsula
Authors: Crist Walter , Abdullayev Rahman
Published: May 2024

The game of fifty-eight holes is one of the longest recognized games of antiquity, but also one of the least understood. New evidence from the Caspian littoral points to an early adoption of the game by Middle Bronze Age seasonally pastoral cattle herders in the late third millennium and early second millennium bc. Six boards bearing this game's distinct pattern were found at sites on the Abşeron Peninsula and Gobustan Reserve in Azerbaijan. Their presence there not only indicates that the region was connected to societies to the south, but also demonstrates the game's popularity across cultures and socioeconomic groups. Its supposed first appearance in Egypt is questioned in favour of a south-western Asian origin.
The Concept of the Rule of Law and Global Economic Governance: Theoretical Remarks
Authors: Culot Henri
Published: May 2024

This article offers some general thoughts on the rule of law in international economic law. It begins by briefly defining the rule of law and indicating the legal sources on which it is based. It then shows that the TFEU, confirmed by the case law of the Court of Justice, requires the rule of law to be respected in the conduct of the Union’s commercial policy. However, although the rule of law may be favourable to international trade and investment, it is not indispensable to them. The rule of law is conducive, but not essential, to trade and investment. For businesses, the risks associated with a weak respect for the rule of law represent a cost, which they take into account when setting the price of their products. Finally, it should be remembered that the principles of the rule of law do not apply in the same way in the domestic sphere as in international law. This article is intended as a panoramic introduction to the relationship between the rule of law and international economic law. More specialised studies are published later in this issue, including analyses focusing on trade or investment or national perspectives, such as that of China.
Fluctuation covariance-based study of roll-streak dynamics in Poiseuille flow turbulence
Authors: Nikolaidis Marios-Andreas , Ioannou Petros J. , Farrell Brian F.
Published: May 2024

Although the roll-streak (R-S) is fundamentally involved in the dynamics of wall turbulence, the physical mechanism responsible for its formation and maintenance remains controversial. In this work we investigate the dynamics maintaining the R-S in turbulent Poiseuille flow at

$R=1650$

. Spanwise collocation is used to remove spanwise displacement of the streaks and associated flow components, which isolates the streamwise-mean flow R-S component and the second-order statistics of the streamwise-varying fluctuations that are collocated with the R-S. This partition of the dynamics into streamwise-mean and fluctuation components facilitates exploiting insights gained from the analytic characterization of turbulence in the second-order statistical state dynamics (SSD), referred to as S3T, and its closely associated restricted nonlinear dynamics (RNL) approximation. Symmetry of the statistics about the streak centreline permits separation of the fluctuations into sinuous and varicose components. The Reynolds stress forcing induced by the sinuous and varicose fluctuations acting on the R-S is shown to reinforce low- and high-speed streaks, respectively. This targeted reinforcement of streaks by the Reynolds stresses occurs continuously as the fluctuation field is strained by the streamwise-mean streak and not intermittently as would be associated with streak-breakdown events. The Reynolds stresses maintaining the streamwise-mean roll arise primarily from the dominant proper orthogonal decomposition (POD) modes of the fluctuations, which can be identified with the time average structure of optimal perturbations growing on the streak. These results are consistent with a universal process of R-S growth and maintenance in turbulent shear flow arising from roll forcing generated by straining turbulent fluctuations, which was identified using the S3T SSD.
Lowering the Age of Consent for Vaccination to Promote Pediatric Vaccination: It’s Worth a Shot
Authors: Irwin Margaret , Soled Derek R. , Cummings Christy L.
Published: May 2024

This paper challenges historically preconceived notions surrounding a minor’s ability to make medical decisions, arguing that federal health law should be reformed to allow minors with capacity as young as age 12 to consent to their own Centers for Diseases Control and Prevention (CDC)-approved COVID-19 vaccinations. This proposal aligns with and expands upon current exceptions to limitations on adolescent decision-making. This analysis reviews the historic and current anti-vaccination sentiment, examines legal precedence and rationale, outlines supporting ethical arguments regarding adolescent decision-making, and offers rebuttals to anticipated ethical counterarguments.
Justice, Labor, Research, and Power: The Significance and Implications of Parent-Reported Outcomes in Medical-Legal Partnership
Authors: Bhandary-Alexander James
Published: May 2024

As a legal aid union president in New Haven, laboring within shouting distance of a different large research university, I recall how our membership rolled our eyes when Professors Greiner, Pattanayak, and Hennesy of Harvard published their study providing evidence, through a randomized control trial, that law clinic housing work made no difference for clients.1 Representing, as I was, “lawyers, secretaries, and paralegals who have dedicated their careers to serving poor clients in crisis,”2 the authors’ conclusion generated first shock, then denial, and then an anxious realization that somebody’s job was to research and disseminate such conclusions. In a 2013 United States where there was one legal aid lawyer for every 8,893 people who qualified,3 where federal Legal Services Corporation funding had dropped 40% over ten years in real dollars,4 and in an America that spends as much on Halloween costumes for its pets as it does legal aid for the poor,5 the inquiry felt like a pile-on. It made no more sense to us than asking if a teacher is “good for students,” a nurse “good for the sick,” or a chef “good for the hungry.”6
Diverting Data and Drugs: A Narrative Review of the Mallinckrodt Documents
Authors: Lentacker Antoine , Pham Kelly , Chernesky Jason M.
Published: May 2024

U.S. law imposes strict recording and reporting requirements on all entities that manufacture and distribute controlled substances. As a result, the prescription opioid crisis has unfolded in a data-saturated environment. This article asks why the systematic documentation of opioid transactions failed to prevent or mitigate the crisis. Drawing on a recently disclosed trove of 1.4 million internal records from Mallinckrodt Pharmaceuticals, a leading manufacturer of prescription opioids, we highlight a phenomenon we propose to call data diversion, whereby data ostensibly generated or collected for the purpose of regulating the distribution of controlled substances were repurposed by the industry for the opposite aim of increasing sales at all costs. Systematic data diversion, we argue, contributed substantially to the scale of drug diversion seen with opioids and should become a focus of policy intervention.
Beyond stomatal development: SMF transcription factors as versatile toolkits for land plant evolution
Authors: Doll Yuki , Koga Hiroyuki , Tsukaya Hirokazu
Published: May 2024

As master transcription factors of stomatal development, SPEECHLESS, MUTE, and FAMA, collectively termed SMFs, are primary targets of molecular genetic analyses in the model plant Arabidopsis thaliana. Studies in other model systems identified SMF orthologs as key players in evolutionary developmental biology studies on stomata. However, recent studies on the astomatous liverwort Marchantia polymorpha revealed that the functions of these genes are not limited to the stomatal development, but extend to other types of tissues, namely sporophytic setal and gametophytic epidermal tissues. These studies provide insightful examples of gene-regulatory network co-opting, and highlight SMFs and related transcription factors as general toolkits for novel trait evolution in land plant lineages. Here, we critically review recent literature on the SMF-like gene in M. polymorpha and discuss their implications for plant evolutionary biology.
Barriers and facilitators to health-care access by older Chinese migrants in high-income countries: a mixed-methods systematic review
Authors: Guo Haoyue , AlSwayied Ghada , Frost Rachael , Rait Greta , Burns Fiona
Published: May 2024

International migrants face barriers when accessing health-care in their destination countries. For older migrants, there are additional difficulties due to their age and associated health conditions. Chinese migrants are an understudied group with culture-specific barriers in addition to those shared with other migrant groups. This review aims to understand the barriers and facilitators to health-care access faced by older Chinese migrants in high-income countries. Literature from MEDLINE, Web of Science, EMBASE, Scopus, CINAHL Plus and ProQuest (1 January 2000 to 6 October 2021) were retrieved. Quantitative, qualitative and mixed-methods studies focusing on older Chinese migrants' access to, utilisation of and satisfaction with health-care services in high-income countries were included. Studies were appraised using checklists from the Joanna Briggs Institute and the Critical Appraisal Skills Programme. Qualitative and quantitative data were extracted and analysed narratively to identify barriers and facilitators to accessing health-care, then applied to Levesque's five-step health-care access journey framework. We included 33 studies in the analysis. Qualitative evidence identified barriers and facilitators to health-care access in four categories: health-care system, social factors, personal factors and health-care interactions. Quantitative studies found that health status and having insurance were positively associated with using non-preventive care, while time of residence and physician's recommendations were positively associated with using preventive care. Factors that influence older Chinese migrants' access to health care include practical barriers (communication, time and cost), social support (family and community), perceptions of health and care needs (beliefs and knowledge) and interactions with health-care professionals (patient–physician trust and support from physicians). Efforts to overcome universal barriers, acknowledgement of cultural contexts, improvements in translation services, and involvement of Chinese families and communities in health-care outreach will benefit this population.
SPURIOUS FACTORS IN DATA WITH LOCAL-TO-UNIT ROOTS
Authors: Onatski Alexei , Wang Chen
Published: May 2024

This paper extends the spurious factor analysis of Onatski and Wang (2021, Spurious factor analysis.Econometrica, 89(2), 591–614.) to high-dimensional data with heterogeneous local-to-unit roots. We find a spurious factor phenomenon similar to that observed in the data with unit roots. Namely, the “factors” estimated by the principal components analysis converge to principal eigenfunctions of a weighted average of the covariance kernels of the demeaned Ornstein–Uhlenbeck processes with different decay rates. Thus, such “factors” reflect the structure of the strong temporal correlation of the data and do not correspond to any cross-sectional commonalities, that genuine factors are usually associated with. Furthermore, the principal eigenvalues of the sample covariance matrix are very large relative to the other eigenvalues, creating an illusion of the “factors”capturing much of the data’s common variation. We conjecture that the spurious factor phenomenon holds, more generally, for data obtained from high frequency sampling of heterogeneous continuous time (or spacial) processes, and provide an illustration.
Deep temporal semi-supervised one-class classification for GNSS radio frequency interference detection
Authors: Ivanov Viktor , Scaramuzza Maurizio , Wilson Richard. C.
Published: May 2024

We present a deep learning approach for near real-time detection of Global Navigation Satellite System (GNSS) radio frequency interference (RFI) based on a large amount of aircraft data collected onboard from the Global Positioning System (GPS) and Attitude and Heading Reference System (AHRS). Our approach enables detection of GNSS RFI in the absence of total GPS failure, i.e. while the receiver is still able to estimate a position, which means RFI sources with low power or at larger distance can be detected. We demonstrate how deep one-class classification can be used to detect GNSS RFI. Furthermore, thanks to a unique dataset from the Swiss Air Force and Swiss Air-Rescue (Rega), preprocessed by Swiss Air Navigation Services Ltd. (Skyguide), we demonstrate application of deep learning for GNSS RFI detection on real-world large scale aircraft data containing flight recordings impacted by real jamming. The approach we present is highly general and can be used as a foundation for solving various automated decision-making problems based on different types of Communications, Navigation and Surveillance (CNS) and Air Traffic Management (ATM) streaming data. The experimental results indicate that our system successfully detects GNSS RFI with 83

$\,\cdot\,$

5% accuracy. Extensive empirical studies demonstrate that the proposed method outperforms strong machine learning and rule-based baselines.
Refugees as Resources: A Post-War Experiment in European Refugee Relief
Authors: Ballinger Pamela
Published: May 2024

This article explores the Homeless European Land Program, an experiment in resettling foreign refugees in post-Second World War Sardinia undertaken by two idealistic Americans with the support of the Brethren Service Committee and the fledgling UNHCR. Focusing on individuals rejected for immigration, the initiative aimed to integrate these ‘hard core’ refugees by rendering them agents of development of a ‘backwards’ region of the Italian South and to overcome Italian reluctance to serve as a country of permanent resettlement for the displaced. The history of this project reveals the contradictory impulses of early Cold War refugee relief and humanitarianism: the competition between intergovernmental and voluntary agencies, of secular and spiritual enterprises, and of images of refugees as dependent and difficult to settle and yet capable of self-sufficiency. Many of the ideas piloted in Sardinia, notably the linking of self-sufficiency and development, later became prominent in the UNHCR's work in the Global South.
Target-site mechanism confers resistance pattern of ACCase inhibitors in bearded sprangletop (Leptochloa fusca ssp. fascicularis) from California
Authors: Unan Rasim , Becerra-Alvarez Aaron , Al-Khatib Kassim
Published: May 2024

Bearded sprangletop is a problematic native grass weed in California’s rice fields. The widespread and extensive use of acetyl-CoA carboxylase (ACCase)–inhibiting herbicides, such as cyhalofop-p-butyl (cyhalofop), has led to speculation that biotypes of bearded sprangletop have developed herbicide resistance to ACCase. The aim of this study was to evaluate suspected resistant bearded sprangletop biotypes, R1, R2, R3, and the susceptible biotype, S1, in terms of their levels of resistance to three ACCase-inhibiting herbicides and to characterize the molecular mechanisms of resistance. Dose–response experiments suggested that the biotype R1, R2, and R3 had high-level resistance to cyhalofop and to quizalofop-p-ethyl (quizalofop), but not clethodim. The study determined that the resistance to ACCase inhibitors was a target-site mechanism resulting from nucleotide substitution. The carboxyl transferase (CT) domain of the ACCase gene’s sequence analysis revealed the substitutions Trp-2027-Cys for R1 and R2 biotypes and Ile-2041-Asn for the R3 biotype. This study revealed the presence of target-site resistance to cyhalofop and quizalofop in at least two mutation points in representative biotypes of bearded sprangletop in California. This research highlights the significance of careful herbicide selection due to weed species responding quite rapidly to selection pressure, so as to aid in managing bearded sprangletop in rice fields.
What Controls Early Restraining Bend Growth? Structural, Morphometric, and Numerical Modeling Analyses From the Eastern California Shear Zone
Authors: Garvue Max M. , Spotila James A. , Cooke Michele L. , Curtiss Elizabeth R.
Published: May 2024

Restraining bends influence topography, strike‐slip evolution, and earthquake rupture dynamics, however the specific factors governing their geometry and development in the crust are not well established. These relationships are challenging to investigate in field examples due to cannibalization and erosion of earlier structures with cumulative strain. To address this knowledge gap, we investigated the structure, morphology, and kinematics of 22 basement‐cored restraining bends on low net‐slip faults (<10 km) within the southern Eastern California shear zone (SECSZ) via mapping, topographic analyses, and 3D numerical modeling. The bends are self‐similar in form with most exhibiting focused relief between high‐angle bounding faults with an arrowhead shape in map view and a “whaleback” longitudinal profile. Slight changes in that form occur with increasing size indicating predictable growth that appears to be primarily controlled by local fault geometries (i.e., bifurcation angle), rather than the influence of fault obliquity relative to far‐field plate motion, due to inefficient slip‐transfer across interconnected irregularly trending closely spaced faults. Modeling results indicate that the self‐similar fault‐bound geometry of SECSZ restraining bends may arise from elevated shear strain at the outer corners of single transpressional fault bends with increasing cumulative slip. This, in turn, promotes growth of a new fault leading to efficient accommodation of local convergent strain via uplift between bounding faults. Finally, our results indicate that the kilometer‐scale restraining bends contribute minimally to regional contraction as they only penetrate the upper third of the seismogenic crust and are therefore also unlikely to impede large earthquake surface ruptures.
Nitrogen Assimilation and Nitrification in Surface Waters of the Amazon and Pará Estuaries
Authors: Choisnard N. , Umbricht J. , Araujo M. , Böttcher M. E. , Burmeister C. , Liskow I. , Schmiedinger I. , Voss M.
Published: May 2024

Under increasing anthropogenic impacts, the hydrology, weathering, turbidity, and biogeochemistry of the Amazon and Pará river catchments are changing, potentially affecting the concentration and speciation of nitrogen (N) entering the estuary, as well as the subsequent primary productivity. Primary production and several N‐cycling rates have been studied in the northern river plume, and new studies focusing on specific processes in the river mouth emerged. Yet, a general overview is still lacking. For the first time, we provide an overview of primary production along with nitrification, ammonium (NH4+), nitrate (NO3−), and amino acid uptake rates in the Amazon and Pará river mouths which exhibit different degrees of catchment land use. Our results indicate that the Amazon supplies tenfold more NH4+ and NO3−, and its nitrification rates are four times higher than in the Pará River. The stable isotope composition of ambient water and NO3− confirmed that nitrification is a dominant pathway, with fluxes reaching 8.5 × 108 mol N d−1 in the Amazon. Nutrients are transported to regions with reduced turbidity and little nitrification, facilitating NH4+ (0.2–2.4 × 108 mol N d−1) and carbon uptake (4.6 and 14.0 × 108 mol C d−1 in the Pará and the Amazon, respectively) by phytoplankton. The lower N‐cycling rates measured in the Pará relative to the Amazon River mouth likely originate from differences in river discharge and watershed characteristics.
The ion channel TRPA1 is a modulator of the cocaine reward circuit in the nucleus accumbens
Authors: Kim Young-Jung , Choi Su Jeong , Hong Sa-Ik , Park Jung-Cheol , Lee Youyoung , Ma Shi-Xun , Hur Kwang-Hyun , Lee Young , Kim Kyeong-Man , Kim Hyung Kyu , Kim Hee Young , Lee Seok-Yong , Choi Se-Young , Jang Choon-Gon
Published: May 2024

Drug addiction therapies commonly fail because continued drug use promotes the release of excessive and pleasurable dopamine levels. Because the connection between pleasure and drug use becomes hard-wired in the nucleus accumbens (NAc), which interfaces motivation, effective therapies need to modulate this mesolimbic reward system. Here, we report that mice with knockdown of the cation channel TRPA1 (transient receptor potential ankyrin 1) were resistant to the drug-seeking behavior and reward effects of cocaine compared to their wildtype litter mates. In our study, we demonstrate that TRPA1 inhibition in the NAc reduces cocaine activity and dopamine release, and conversely, that TRPA1 is critical for cocaine-induced synaptic strength in dopamine receptor 1-expressing medium spiny neurons. Taken together, our data support that cocaine-induced reward-related behavior and synaptic release of dopamine in the NAc are controlled by TRPA1 and suggest that TRPA1 has therapeutic potential as a target for drug misuse therapies.
Maternal behaviours disrupted by Gprasp2 deletion modulate neurodevelopmental trajectory in progeny
Authors: Pereira Marta I. , Laranjo Mariana , Gomes Marcos , Edfawy Mohamed , Peça João
Published: May 2024

Autism spectrum disorders (ASDs) are known to present sex-specific differences. At the same time, understanding how maternal behaviours are affected by pathogenic mutations is crucial to translate research efforts since rearing may recursively modulate neurodevelopment phenotype of the progeny. In this work, we focused on the effects of Gprasp2 deletion in females and its impact in progeny care and development. Female mice, wild-type (WT), Gprasp2+/− (HET) or Gprasp2−/− (KO) mutants and their progeny were used and behavioural paradigms targeting anxiety, memory, maternal care, and other social behaviours were performed. Analysis of communication was carried out through daily recordings of ultrasonic vocalizations in isolated pups and cross-fostering experiments were performed to understand the effect of maternal genotype in pup development. We found that Gprasp2−/− females presented striking impairments in social and working memory. Females also showed disruptions in maternal care, as well as physiological and molecular alterations in the reproductive system and hypothalamus, such as the structure of the mammary gland and the expression levels of oxytocin receptor (OxtR) in nulliparous versus primiparous females. We observed alterations in pup communication, particularly a reduced number of calls in Gprasp2 KO pups, which resulted from an interaction effect of the dam and pup genotype. Cross-fostering mutant pups with wild-type dams rescued some of the early defects shown in vocalizations, however, this effect was not bidirectional, as rearing WT pups with Gprasp2−/− dams was not sufficient to induce significant phenotypical alterations. Our results suggest Gprasp2 mutations perturb social and working memory in a sex-independent manner, but impact female-specific behaviours towards progeny care, female physiology, and gene expression. These changes in mutant dams contribute to a disruption in early stages of progeny development. More generally, our results highlight the need to better understand GxE interactions in the context of ASDs, when female behaviour may present a contributing factor in postnatal neurodevelopmental trajectory.
Tuning the band gap, optical, mechanical, and electrical features of a bio-blend by Cr2O3/V2O5 nanofillers for optoelectronics and energy applications
Authors: Alanazi Tarek I. , Alenazi Raghad A. , El Sayed Adel M.
Published: May 2024

This work presents a facile approach for controlling the optical and electrical parameters of a biopolymeric matrix for optoelectronics. Vanadium oxide (V2O5) and chromium oxide (Cr2O3) nanoparticles (NPs) were prepared and incorporated into the carboxymethylcellulose/polyethylene glycol (CMC/PEG) blend by simple chemical techniques. Transmission electron microscopy (HR-TEM), and X-ray diffraction (XRD) data showed that V2O5 and Cr2O3 exhibited spherical shapes with sizes in the range of 40–50 nm and 10–20 nm, respectively. In addition, the blend's degree of crystallinity was sensitive to the V2O5 and Cr2O3 doping ratios. The scanning electron microscopy (FE-SEM) and the elemental chemical analysis (EDAX) used to study the filler distribution inside the blend, and confirmed the existence of both V and Cr in the matrix. Fourier transform infrared (FTIR) spectroscopy showed that the dopants significantly affected the blend reactive (C–O–C, OH, and C=O) groups. The stress–strain curves illustrated the reinforcing effect of the dopants up to 1.0 $$\text{wt\%}$$
wt\%
Cr2O3/V. The transmittance and absorption index spectra in the visible-IR wavelengths decreased with increasing filler content. Utilizing Tauc's relation and (optical) dielectric loss, the direct (indirect) band gap narrowed from 5.6 (4.5) eV to 4.7 (3.05) eV at 1.0  $$\text{wt\%}$$
wt\%
Cr2O3/V. All films have an index of refraction in the range of 1.93–2.17. AC conductivity was improved with increasing filler content and temperature. The energy density at 50 °C is in the range of 1–3 J/m3. The influence of V2O5 and Cr2O3 content on the optical conductivity, dielectric constant, loss, and dielectric modulus of CMC/PEG was reported. These enhancements in electrical and optical properties, along with the potential for band gap engineering, offer promising prospects for advanced applications in optoelectronics and energy-related fields.
Spatial diversity of atmospheric moisture transport and climate teleconnections over Indian subcontinent at different timescales
Authors: Raghuvanshi Akash Singh , Agarwal Ankit
Published: May 2024

Regional weather and climate are generally impacted by global climatic phenomenon′s. Understanding the impact of global climate phenomenon′s on an atmospheric branch of the hydrological cycle is crucial to make advances in skillful precipitation forecast. The present study adopts a multiscale approach based on wavelets for unravelling the linkages between teleconnections and atmospheric moisture transport over homogeneous regions of Indian sub-continent. We investigated linkages between atmospheric moisture transport quantified as monthly integrated water vapor transport (IVT) during 1951–2022 over selected homogeneous regions and eight large scale climate oscillations using wavelet and global wavelet coherence. Our results indicate significant heterogeneity in linkages across different regions and across multiple timescales. In particular, the Indian Ocean Dipole (IOD) influence monthly IVT at intra-annual to inter-annual scale over all regions. The El Niño–Southern Oscillation (ENSO) have strong connection to monthly IVT at inter-annual scale whereas over west central region both IOD and ENSO strongly influence IVT at inter-decadal scale. While the Atlantic Multi-Decadal Oscillation and Pacific Decadal Oscillation have an impact on IVT in the north-east and southern regions, the Arctic Oscillation and North Atlantic oscillation have a strong inter-annual connection to IVT, majorly in the northwest and hilly regions. Overall, the methodology offers an effective approach for capturing the dynamics of atmospheric moisture transport in time–frequency space and provide a practical reference for prediction of atmospheric moisture transport linked precipitation over different regions of Indian subcontinent.
Bioconversion of cow manure through vermicomposting: effects of tylosin concentration on the weight of worms and manure quality
Authors: Ghandehari Yazdi Farnaz , Mokhtari Mehdi , Nabi Meibodi Mohsen , Sefidkar Reyhane , Hatami Behnam , Molavi Fereshteh , Ghafourzadeh Mahin , Golshiri Ahmad , Ebrahimi Ali Asghar
Published: May 2024

This study investigated batch-fed vermicomposting of cow manure, with a specific focus on assessing the effects of tylosin on the weight of earthworms and the overall quality of the resulting manure. Five reactors, including three concentrations of tylosin (50, 100, and 150 mg/kg) and two control reactors, were employed. Residual tylosin concentrations were measured using high-performance liquid chromatography (HPLC). Quality parameters such as pH, temperature, volatile solids (VS), organic carbon content (OCC), electrical conductivity (EC), ash content, C/N ratio, total Kjeldahl nitrogen (TKN), and microbial content were evaluated. The toxicity and maturity of vermicompost were assessed by determining the germination index (GI). The study also monitored variations in the earthworm’s weight. The results demonstrated a decreasing trend in VS, OCC, C/N, and fecal coliforms, along with increased pH, EC, ash content, and TKN during the vermicomposting process. Furthermore, investigations revealed significant reductions in the reactors with tylosin concentrations of 50, 100, and 150 mg/kg, resulting in the removal of 98%, 90.48%, and 89.38% of the initial tylosin, respectively. This result confirms the faster removal of tylosin in reactors with lower concentrations. Degradation of tylosin also conforms to first-order kinetics. The findings showed a significant influence of tylosin on the weight of Eisenia fetida earthworms and the lowest antibiotic concentration led to the highest weight gain. Finally, the high percentage of germination index (90–100%) showed that the quality and maturity of vermicompost is by national and international standards.
Comprehensive expression analysis of hormone-like substances in the subcutaneous adipose tissue of the common bottlenose dolphin Tursiops truncatus
Authors: Suzuki Miwa , Funasaka Noriko , Yoshimura Kazuma , Inamori Daiki , Watanabe Yurie , Ozaki Miki , Hosono Masayuki , Shindo Hideaki , Kawamura Keiko , Tatsukawa Toshiyuki , Yoshioka Motoi
Published: May 2024

Marine mammals possess a specific subcutaneous fat layer called blubber that not only insulates and stores energy but also secretes bioactive substances. However, our understanding of its role as a secretory organ in cetaceans is incomplete. To exhaustively explore the hormone-like substances produced in dolphin subcutaneous adipose tissue, we performed seasonal blubber biopsies from captive female common bottlenose dolphins (Tursiops truncatus; N = 8, n = 32) and analyzed gene expression via transcriptomics. Analysis of 186 hormone-like substances revealed the expression of 58 substances involved in regulating energy metabolism, tissue growth/differentiation, vascular regulation, immunity, and ion/mineral homeostasis. Adiponectin was the most abundantly expressed gene, followed by angiopoietin protein like 4 and insulin-like growth factor 2. To investigate the endocrine/secretory responses of subcutaneous adipose tissue to the surrounding temperature, we subsequently compared the mean expression levels of the genes during the colder and warmer seasons. In the colder season, molecules associated with appetite suppression, vasodilation, and tissue proliferation were relatively highly expressed. In contrast, warmer seasons enhanced the expression of substances involved in tissue remodeling, immunity, metabolism, and vasoconstriction. These findings suggest that dolphin blubber may function as an active secretory organ involved in the regulation of metabolism, appetite, and tissue reorganization in response to changes in the surrounding environment, providing a basis for elucidating the function of hormone-like substances in group-specific evolved subcutaneous adipose tissue.
Predicting mortality after transcatheter aortic valve replacement using preprocedural CT
Authors: Brüggemann David , Kuzo Nazar , Anwer Shehab , Kebernik Julia , Eberhard Matthias , Alkadhi Hatem , Tanner Felix C. , Konukoglu Ender
Published: May 2024

Transcatheter aortic valve replacement (TAVR) is a widely used intervention for patients with severe aortic stenosis. Identifying high-risk patients is crucial due to potential postprocedural complications. Currently, this involves manual clinical assessment and time-consuming radiological assessment of preprocedural computed tomography (CT) images by an expert radiologist. In this study, we introduce a probabilistic model that predicts post-TAVR mortality automatically using unprocessed, preprocedural CT and 25 baseline patient characteristics. The model utilizes CT volumes by automatically localizing and extracting a region of interest around the aortic root and ascending aorta. It then extracts task-specific features with a 3D deep neural network and integrates them with patient characteristics to perform outcome prediction. As missing measurements or even missing CT images are common in TAVR planning, the proposed model is designed with a probabilistic structure to allow for marginalization over such missing information. Our model demonstrates an AUROC of 0.725 for predicting all-cause mortality during postprocedure follow-up on a cohort of 1449 TAVR patients. This performance is on par with what can be achieved with lengthy radiological assessments performed by experts. Thus, these findings underscore the potential of the proposed model in automatically analyzing CT volumes and integrating them with patient characteristics for predicting mortality after TAVR.
The effect of duration between sessions on microperimetric biofeedback training in patients with maculopathies
Authors: Zhou Jie , Hou Jintong , Li Shengnan , Zhang Jinglin
Published: May 2024

Aim of this study was to explore the optimal training interval and times of microperimetric biofeedback training (MBFT) in maculopathies. Twenty-nine patients with maculopathies were divided into two groups: daily training (Group A) or alternate daily training (Group B). Both groups underwent 15 MBFT sessions. We compared the BCVA, reading speed, and fixation stability at baseline, after 5, 10, 15 sessions. After 15 sessions of MBFT, all visual parameters in both groups improved. There was a significant increase in BCVA after 5 sessions in both groups (P=0.016, and P<0.001 respectively), but Group A showed further improvement after 10 sessions (P<0.001). Regarding reading speed, Group A showed significant improvement from baseline after 15 sessions(P=0.020), Group B improved significantly after 5 sessions (P=0.047) and continued to improve after 10 sessions (P=0.030). Additionally, P1 and LgBCEA of Group A significantly improved after 10 sessions (P=0.001, and P=0.001 respectively), while Group B significantly improved after 5 sessions (P=0.002, and P<0.001 respectively). There was no significant difference in visual outcomes between the two groups (P>0.05) except LgBCEA (P=0.046) after 15 sessions. We concluded that the both MBFT frequencies are effective at improving vision and quality of life in patients with maculopathies. The alternate daily training group showed less time-dependent of improvement in all parameters and a greater benefit in fixation stability. Ten sessions are the optimal number of treatment sessions for alternate daily training.
Integrated high-precision monitoring method for surface subsidence in mining areas using D-InSAR, SBAS, and UAV technologies
Authors: Zhu Mingfei , Yu Xuexiang , Tan Hao , Yuan Jiajia
Published: May 2024

The use of unmanned operations to monitor mining induced land subsidence is increasing. This study conducts a detailed comparative analysis of accuracy of measured ground deformation provided by Differential Interferometric Synthetic Aperture Radar (D-InSAR), Small Baseline Subset (SBAS), and Unmanned Aerial Vehicle (UAV) tilt photogrammetry with respect to levelling measurements. Based on such analysis we propose an integrated approach that combines multiple remote sensing methods to achieve a better global accuracy in the land subsidence monitoring in mining areas. Conducted at the Banji Coal Mine, this study collected subsidence data from April 10, 2021, to June 28, 2022, through D-InSAR, SBAS, and UAV techniques. After segmenting the subsidence basin into distinct zones, we qualitatively assessed each area with UAV-derived 3D models and quantitatively evaluated the precision of all applied techniques, benchmarking against leveling data. Our findings indicate that integrating D-InSAR, SBAS, and UAV technologies significantly enhances monitoring accuracy over any single method, demonstrating their combined effectiveness in different subsidence areas. Consequently, the synergistic integration of D-InSAR, SBAS, and UAV technologies, capitalizing on their complementary strengths, enables the achievement of intuitive, comprehensive, and high-precision monitoring of subsidence basins in mining areas.