bluuebunny/crossref_metadata_2025 huggingface dataset:
Mental Health and Well-Being Needs among Non-Health Essential Workers during Recent Epidemics and Pandemics
Authors: Chowdhury Nashit , Kainth Ankit , Godlu Atobrhan , Farinas Honey Abigail , Sikdar Saif , Turin Tanvir C.
Published: May 2022

Essential workers, those who work in a variety of sectors that are critical to sustain the societal infrastructure, were affected both physically and mentally by the COVID-19 pandemic. While the most studied group of this population were healthcare workers, other essential non-health workers such as those working in the law enforcement sector, grocery services, food services, delivery services, and other sectors were studied less commonly. We explored both the academic (using MEDLINE, PsycInfo, CINAHL, Sociological Abstracts, and Web of Science databases) and grey literature (using Google Scholar) to identify studies on the mental health effects of the six pandemics in the last 20 years (2000–2020). We identified a total of 32 articles; all of them pertained to COVID-19 except for one about Ebola. We found there was an increase in depression, anxiety, stress, and other mental health issues among non-health essential workers. They were more worried about passing the infection on to their loved ones and often did not have adequate training, supply of personal protective equipment, and support to cope with the effects. Generally, women, people having lower education, and younger people were more likely to be affected by a pandemic. Exploring occupation-specific coping strategies of those whose mental health was affected during a pandemic using more robust methodologies such as longitudinal studies and in-depth qualitative exploration would help facilitate appropriate responses for their recovery.
Changes in the Circulation of Common Respiratory Pathogens among Hospitalized Patients with Influenza-like Illnesses in the Lazio Region (Italy) during Fall Season of the Past Three Years
Authors: Sberna Giuseppe , Lalle Eleonora , Valli Maria Beatrice , Bordi Licia , Garbuglia Anna Rosa , Amendola Alessandra
Published: May 2022

A descriptive analysis of common respiratory pathogens (CRPs) detected in nasopharyngeal swabs (NPSs) from hospitalized patients with influenza-like illness during the fall seasons of the past three years, 2019–2021, in the Lazio region, Italy, was conducted to assess whether or not CRP circulation changed because of COVID-19 during the fall season. The results observed in a total of 633 NPSs subjected to molecular diagnosis for CRPs by multiplex PCR assay during the autumn seasons (exactly from week 41 to week 50) were compared with each other. In 2019, in 144 NPSs, the more represented CRPs were rhinovirus/enterovirus (7.6%) and influenza A/B (4.2%). In 2020, 55 (21.6%) out of 255 NPSs resulted positive for SARS-CoV-2 and, except for one case of Legionella pneumophila, the CRPs detected were exclusively rhinovirus/enterovirus (4.7%). In 2021, among 234 NPSs, 25.6% resulted positive for SARS-CoV-2, 14.5% for respiratory syncytial virus (RSV), and 12.8% for rhinovirus/enterovirus. Compared with 2019, in 2020, CRP circulation was severely limited to a few cases; in 2021, instead, infections by RSV (detected also among adults), rhinovirus/enterovirus, and other respiratory pathogens were observed again, while influenza was practically absent. The comparison of the CRPs detected in the NPSs depicts a different circulation in the Lazio region during the last three fall seasons. CRP monitoring has a direct impact on the prevention and control strategies of respiratory infectious diseases, such as the non-pharmacological interventions implemented in response to the COVID-19 pandemic. Future studies should investigate the impact of specific interventions on the spread of respiratory infections.
Caring and Working during the COVID-19 Pandemic: Perspective of Portuguese Residential Care Facility Workers
Authors: Barbosa Maria Miguel , Teixeira Laetitia , Paúl Constança , Yanguas Javier , Afonso Rosa Marina
Published: May 2022

Residential care facilities (RCF) for older people are facing high demands due to the COVID-19 pandemic. The aim of this study was to explore the workers’ perspectives on the changes in work and care dynamics amidst the first wave of the pandemic at Portuguese RCF. This is a descriptive, quantitative, and cross-sectional study. An online questionnaire about pandemic-induced changes in work and care dynamics was sent to 2325 RCF. These entities were then asked to share it with their workers. The participants (n = 784) were mostly women (92.7%) and mostly composed of technical directors (41.6%) and direct-care workers (17.1%). The respondents reported that during the first wave of the pandemic, when compared to the pre-pandemic period, there were greater difficulties in providing care related to the basic necessities of older people (52.7%); direct-care workers were required to work more consecutive hours in each shift (69.95%); direct-care workers had to live at RCF (14.8%), and there were changes concerning the possibility of promoting person-centered care (PCC) practices. It also revealed that focusing on disease prevention and sanitary measures alone facilitates practices that reinforce the traditional model of procedure-centered care and have negative consequences on the rights and well-being of those living and working at RCF, exposing and accentuating preexisting vulnerabilities. This study considers the pandemic’s serious implications and alarming questions about basic care, dignity, living, and working conditions at Portuguese RCF. These notions reinforce the need for change through redefining care policies and practices in Portuguese RCF beyond the pandemic. The current situation provides an opportunity to adopt a formal PCC model.
An Assessment of the Early Symptoms of Energy Deficiency as a Female Athlete Triad Risk among the Polish National Kayaking Team Using LEAF-Q
Authors: Witkoś Joanna , Błażejewski Grzegorz , Gierach Marcin
Published: May 2022

Background: Kayaking is a high intensity sport that demands high levels of aerobic and anaerobic capacity as well as a great deal of strength and endurance. The aim of this study was an assessment of the frequency of occurrence of early low energy availability symptoms using the Low Energy Availability in Females Questionnaire for women belonging to the Polish national kayaking team. Additionally, quantitative measurements of body composition and levels of calcium were performed. Methods: The study involved 33 women who were competitors in the Polish national kayaking team. Results: An analysis of the results in terms of disorders in the monthly cycle in the tested kayakers found that only five competitors, approximately 15% of the entire group, had this type of problem. Between the participants who had disorders of the menstrual cycle and those whose cycle was normal, there was only a statistically significant difference at the level of p < 0.001 in relation to the age of the kayakers. Conclusions: The Low Energy Availability in Females Questionnaire proved to be a useful screening tool, which allowed for the early detection of Female Athlete Triad symptoms in several young female kayakers from the Polish national team.
Depression, Anxiety, and Stress among Teachers during the Second COVID-19 Wave
Authors: Lizana Pablo A. , Lera Lydia
Published: May 2022

There is a strong background indicating that the teaching profession is one of the most stressful and that their mental health has deteriorated even further during the pandemic. However, there is a little background about the impact of the COVID-19 infection peaks and teachers’ mental health. To this end, 313 teachers were recruited. Via online questionnaires, an evaluation was performed on their depression, anxiety, and stress symptoms on the DASS-21 scale. Teachers’ sociodemographic and socio-personal data were also analyzed. A binary logistic regression was used to analyze the variables which could be associated with each of the symptoms. High rates of depression, anxiety, and stress symptoms were observed among teachers (67%, 73%, and 86%, respectively). Among teachers who were affected by the work–family balance (89%), there was also an increased risk of symptoms of anxiety (OR: 3.2) and stress (OR: 3.5). Depression symptom risk was higher among women (OR: 2.2), and teachers under 35 years old had a risk of presenting all three symptoms (depression OR: 2.2; anxiety OR: 4.0; stress OR 3.0). In contrast, teaching in private educational establishments was a protective factor for anxiety symptoms (OR: 0.3). The results suggest that the second COVID-19 wave profoundly affected teachers’ mental health. Urgent interventions are thus needed to aid teachers’ mental health.
Understanding the Influence of Community-Level Determinants on Children’s Social and Emotional Well-Being: A Systems Science and Participatory Approach
Authors: Poon Brenda T. , Atchison Chris , Kwan Amanda
Published: May 2022

Healthy social and emotional development and longer-term outcomes for children are shaped by factors across the multiple levels (micro, meso, exo, macro) of a child’s environment. By employing a novel systems science and participatory approach, we were able to co-produce a series of causal loop diagrams that detail the complex relationships between variables operating at the community or neighborhood environment level (e.g., features of the built environment such as: housing type, access, availability, and location; parks and greenspace, facilities such as community services, and other service infrastructure such as transit), and highlight the individual and collective impacts these relationships can have on the subsystem surrounding a child’s social and emotional well-being. Our approach provides a unique lens of knowledge through which communities can identify key leverage points for action and (re)design of community spaces, practices, and policy.
State Health Department Communication about Long COVID in the United States on Facebook: Risks, Prevention, and Support
Authors: Laestadius Linnea I. , Guidry Jeanine P. D. , Bishop Andrea , Campos-Castillo Celeste
Published: May 2022

Greater public awareness of long COVID severity and susceptibility is needed to support those with long COVID and encourage preventive behaviors. It is not yet known to what extent health departments have informed the public about long COVID risks or offered guidance and support for those with long COVID. The objective of this research was to determine how and to what extent US state health departments have communicated with the public about long COVID via Facebook. Facebook posts with COVID-19 and long COVID terms made by 50 US state health departments plus Washington, DC, from 1 January 2020 to 31 January 2022, were collected using CrowdTangle. The first long COVID post appeared on 15 July 2020. From 15 July 2020 to 31 January 2022, state health departments made 49,310 COVID-19 posts and 137 long COVID posts. Using quantitative content analysis methods, long COVID posts were coded for health belief model constructs. Among long COVID posts, 75.18% included language about susceptibility, 64.96% severity, and 64.23% benefits of prevention. Cues to preventive action appeared in 54.01% of posts. 19.71% of posts provided guidance for those with long COVID. While health departments posted extensively about COVID-19, posts about long COVID were rare. This represents a missed opportunity to bolster arguments for preventive behaviors and support those experiencing long COVID.
Resource and Environmental Pressures on the Transformation of Planting Industry in Arid Oasis
Authors: Huang Jing , Xue Dongqian , Wang Chuansheng , Chen Jiehu
Published: May 2022

Controlling environmental pollutant discharge and water resource demand is crucial for the sustainable development of agriculture and rural areas in arid oases. Taking Ganzhou, an arid oasis in Northwest China, as an example, we established an analysis framework for the relationship between the planting industry transformation and the resource and environmental pressures, from 2011 to 2020, through the methods of inventory, coefficient and quota accounting. The results showed that the planting scale of crops in oases has continuously expanded, with a structural dominance of corn seed production. Pollutant discharge showed a “Z”-type evolution trend, and the demand for water consumption continued to increase. The transformation of the planting industry and pollutant discharge showed coupled trade-offs and a synergetic alternating fluctuations coupling relationship, which was highly co-evolutionary with the demand for water resources. Crop planting exhibited four spatial patterns, namely the mixed planting area of grain and cash crops grown in mountain areas (GCPA), suburban scale vegetable planting (SVPA), planting of seed production corn (MSPA), and the compound planting area of grain crops, oil crops, vegetables, and other characteristic crops (CMPA). MSPA and SVPA had the highest total and average volume per unit area, respectively. The planting industry transformation and evolution of resource and environment pressures are closely related to changes in national strategies, regional agricultural policies, and environmental regulations. Therefore, studying their relationships provides a scientific basis for the formulation of suitable countermeasures, according to the development stage of a region.
Impact of Digital Economy on the Provision Efficiency for Public Health Services: Empirical Study of 31 Provinces in China
Authors: Lyu Yuwen , Peng Yuqing , Liu Hejian , Hwang Ji-Jen
Published: May 2022

The digital economy is booming in China and has become the world’s largest after the United States’. Since China entered the era of the digital economy, its digital technology has radiated into various fields. This study is to examine the impact of China’s digital economy on the provision efficiency of public health institutions and the mechanism of action between them. Specifically, it measures the development level of China’s digital economy, and the provision efficiency of public health institutions from 2009 to 2018. The research also explores the relationship between China’s digital economy and its provision efficiency, through the Tobit-DEA model. An analysis of the regional heterogeneity indicated that the performance of China’s digital economy in the eastern region has a significant positive effect on improving the efficiency of the public health sector. This further confirms that the digital economy has strengthened China’s ability to deal with public health crises during the COVID-19 pandemic. A further mediation effect analysis showed that China’s digital economy optimizes the efficiency of public health provision by improving governmental performance and regulatory quality. This shows that the development of the digital economy promotes the construction of digital government, and thus improves the quality of governmental supervision and governmental performance, which has a significant positive effect on the efficiency of the supply of public health services. During the COVID-19 pandemic especially, government delivery of public health services was critical in addressing public health crises. Therefore, based on the results of our empirical analysis, this study provides policy suggestions for improving the efficiency of public health service provision in the era of the digital economy.
The Challenges of Treating Glucokinase MODY during Pregnancy: A Review of Maternal and Fetal Outcomes
Authors: Kirzhner Alena , Barak Oren , Vaisbuch Edi , Zornitzki Taiba , Schiller Tal
Published: May 2022

Background: The optimal treatment strategy for the follow-up and management of women with glucokinase maturity-onset diabetes of the young (GCK−MODY)during pregnancy remains unknown. Data regarding maternal and fetal outcomes are lacking. Aim: This paper summarizes the existing literature regarding the maternal and fetal outcomes of women with glucokinase MODY to guide future treatment strategy. Methods: A literature search was conducted in Pubmed, Embace, and Cochrane library with citation follow-up using the terms: glucokinase, MODY, diabetes, pregnancy, gestation, and outcomes. We searched for articles with known fetal mutational status. Relevant outcomes included: birthweight, large for gestational age (LGA), small for gestational age (SGA), macrosomia, cesarean delivery (CD), shoulder dystocia, congenital anomalies, miscarriages, preterm births, and long-term outcomes. Results: Fourteen relevant manuscripts were identified describing maternal and fetal outcomes. The percentage of LGA and macrosomia in 102 glucokinase -unaffected offspring (GCK−) was significantly higher than in the glucokinase -affected offspring (GCK+) (44% vs. 10%, p < 0.001 and 22% vs. 2%, p < 0.001, respectively). Among the 173 GCK(+) offspring, only 5% were SGA, which can be expected according to the normal distribution. We observed higher rates of CD and shoulder dystocia in the GCK(−) offspring. Conclusions: GCK(−) offspring have significantly higher birthweights and more birth complications. The optimal treatment strategy to guide management should take into consideration multiple variables other than fetal mutational status.
Employees’ Green Enterprise Motivation and Green Creative Process Engagement and Their Impact on Green Creative Performance
Authors: Hu Xiao , Khan Shumaila Mazhar , Huang Shijiao , Abbas Jawad , Matei Mirabela Constanta , Badulescu Daniel
Published: May 2022

Based on the recent surge in environmental degradation issues mainly caused by the manufacturing industry and the inadequacy of the measures taken to respond to them, this research focuses on investigating whether employees’ motivation to protect the natural environment leads to their green creative performance (GCP) at work. It also examines the role of green creative process engagement (GCPE) as a mediator between green motivation (GM) and GCP. Structural equation modeling (SEM) is used to examine the hypotheses which indicated that employees’ GCP appears to be significantly influenced by their GM. Moreover, GCPE is also found to act as a mediating factor between the two. Moreover, industry type and gender are found to play significant roles in the studied variables. The current research is among the pioneer studies that focus on involving employees in the pro-environmental creative process through green motivation, leading towards GCP, an essential element for achieving the United Nations Sustainable Development Goals. The study’s findings can help companies promote GCP to solve today’s most pressing environmental issues and achieve sustainable development goals.
Carbon Emissions of the Tourism Telecoupling System: Theoretical Framework, Model Specification and Synthesis Effects
Authors: Duan Xiaofang , Zhang Jinhe , Sun Ping , Zhang Honglei , Wang Chang , Sun Ya-Yen , Lenzen Manfred , Malik Arunima , Cao Shanshan , Kan Yue
Published: May 2022

The flows of people and material attributed to international tourism exert a major impact on the global environment. Tourism carbon emissions is the main indicator in this context. However, previous studies focused on estimating the emissions of destinations, ignoring the embodied emissions in tourists’ origins and other areas. This study provides a comprehensive framework of a tourism telecoupling system. Taking China’s international tourism as an example, we estimate the carbon emissions of its tourism telecoupling system based on the Tourism Satellite Account and input–output model. We find that (1) the proposal of a tourism telecoupling system provides a new perspective for analyzing the carbon emissions of a tourism system. The sending system (origins) and indirect spillover system (resource suppliers) have been ignored in previous studies. (2) In the telecoupling system of China’s international tourism, the emission reduction effect of the sending system is significant. (3) The direct spillover system (transit) and indirect spillover system’s spatial transfer effects of environment responsibility are remarkable. (4) There is a large carbon trade implied in international tourism. This study makes us pay attention to the carbon emissions of tourists’ origins and the implied carbon trading in tourism flows.
Application of Integrated Fixed-Film Activated Sludge in a Conventional Wastewater Treatment Plant
Authors: Kuśnierz Magdalena , Domańska Magdalena , Hamal Kamila , Pera Agnieszka
Published: May 2022

It is often only at the operation stage of a wastewater treatment plant that there is a need to adjust the treatment process in terms of variable hydraulic capacity, increased pollutant load, high/low concentration of suspended biomass, or the unfavorable phenomenon of reduced sedimentation capacity of the activated sludge. One of the ways to improve the treatment process efficiency is to increase the biologically active surface by using bio-carriers in the form of fibers, materials, or bio-balls. This paper presents the results of a wastewater treatment plant operation during the period of six months after the implementation of the integrated fixed-film activated sludge (IFAS) technology. The research showed that microorganisms developed both in the activated sludge and on the fibers, positively influencing the activated sludge condition. During the start-up of the IFAS process, ciliates predominated over the other species. However, as oxygen content was high (2 mg/dm3 and more) and textile beds were used, the protozoan population developed intensively, and small metazoans became increasingly common. Throughout the research period, nitrifying and phosphorus-accumulating bacteria were observed both in the activated sludge and on the fibers. Between the 59th and 184th day of operation, numerous microorganisms were detected on the fibers and in the activated sludge, testifying to low biological oxygen demand, good aerobic conditions for nitrification, and long sludge age. However, the process seemed to break down after day 72, when the occurrence of metazoan led to reduced sludge production; after day 88, chemical oxygen demand and total suspended solids in the outflow increased, and oligochaetes and rotifers dominated the suspended sludge and fibers. Results also showed that the textile bed and low ammonia concentration became an excellent substrate for the development of Stentor sp. With regard to chemical and biological oxygen demand, total nitrogen- and total phosphorus-effluent concentrations were mostly within the legally permissible limits throughout the 184 days of operation.
Mapping Building-Based Spatiotemporal Distributions of Carbon Dioxide Emission: A Case Study in England
Authors: Zheng Yue , Ou Jinpei , Chen Guangzhao , Wu Xinxin , Liu Xiaoping
Published: May 2022

The spatiotemporal inventory of carbon dioxide (CO2) emissions from the building sector is significant for formulating regional and global warming mitigation policies. Previous studies have attempted to use energy consumption models associated with field investigations to estimate CO2 emissions from buildings at local scales, or they used spatial proxies to downscale emission sources from large geographic units to grid cells for larger scales. However, mapping the spatiotemporal distributions of CO2 emissions on a large scale based on buildings remains challenging. Hence, we conducted a case study in England in 2015, wherein we developed linear regression models to analyze monthly CO2 emissions at the building scale by integrating the Emissions Database for Global Atmospheric Research, building data, and Visible Infrared Imaging Radiometer Suite night-time lights images. The results showed that the proposed model that considered building data and night-time light imagery achieved the best fit. Fine-scale spatial heterogeneity was observed in the distributions of building-based CO2 emissions compared to grid-based emission maps. In addition, we observed seasonal differences in CO2 emissions. Specifically, buildings emitted significantly more CO2 in winter than in summer in England. We believe our results have great potential for use in carbon neutrality policy making and climate monitoring.
The Long-Term Effects of Early Childhood Resilience Profiles on School Outcomes among Children in the Child Welfare System
Authors: Yoon Susan , Pei Fei , Benavides Juan Lorenzo , Ploss Alexa , Logan Jessica , Hamby Sherry
Published: May 2022

This study aimed to examine the association between early childhood resilience profiles and later school outcomes (academic achievement and school involvement) among children in the U.S. child welfare system. This study compared 827 children aged 3–5 years in three latent profile groups (poor emotional and behavioral resilience, low cognitive resilience, and multi-domain resilience) to their baseline profiles using data from the National Survey of Child and Adolescent Well-Being (NSCAW-II). At the three-year follow-up, children with low emotional and behavioral resilience profiles and children with the multi-domain resilience profile had significantly higher basic reading skills, reading comprehension, and math reasoning compared to children with low scores on the cognitive resilience profile. Furthermore, children with the multi-domain resilience profile had significantly higher levels of emotional school engagement than did those with the low emotional and behavioral resilience profile and considerably higher levels of behavioral school engagement compared to those with the low cognitive resilience profile. The findings highlight the persistent effects of early resilience into the later childhood years. Moreover, our results suggest the need for early identification of and intervention for children with low cognitive or emotional/behavioral resilience during the preschool years to promote academic success and school engagement during the school-age years.
Ambient Air Pollution and Risk of Admission Due to Asthma in the Three Largest Urban Agglomerations in Poland: A Time-Stratified, Case-Crossover Study
Authors: Dąbrowiecki Piotr , Chciałowski Andrzej , Dąbrowiecka Agata , Badyda Artur
Published: May 2022

Ambient air pollution in urban areas may trigger asthma exacerbations. We carried out a time-series analysis of the association between the concentrations of various air pollutants and the risk of hospital admission due to asthma over 7 days from exposure. We used distributed lag nonlinear models to analyze data gathered between 2010 and 2018 in the three largest urban agglomerations in Poland. Overall, there were 31,919 asthma hospitalizations. Over 7 days since exposure, the rate ratio (95%CI) for admission per 10 µg/m3 was 1.013 (1.002–1.024) for PM10; 1.014 (1.000–1.028) for PM2.5; 1.054 (1.031–1.078) for NO2; and 1.044 for SO2 (95%CI: 0.986–1.104). For all pollutants, the risk of admission was the greatest on the day of exposure (day 0), decreased below baseline on days 1 and 2, and then increased gradually up to day 6. The proportions (95%CI) of hospitalizations attributable to air pollution were 4.52% (0.80%–8.14%) for PM10; 3.74% (0.29%–7.11%) for PM2.5; 16.4% (10.0%–21.8%) for NO2; and 2.50% (−0.75%–5.36%) for SO2. In conclusion, PM2.5, PM10, NO2, and SO2 pollution was associated with an increased risk of hospital admission due to asthma in the three largest urban agglomerations in Poland over nine years.
Examining Adolescent Tennis Participation in Contemporary China Using an Ecological Framework
Authors: Li Longxi , Rick Oliver J. C. , Mullin Elizabeth M. , Moosbrugger Michelle E.
Published: May 2022

Physical activity and sport participation behaviors in children and adolescents are consistently shaped by surrounding ecological systems. Accumulating evidence highlights individual, family, peer, school and teacher, and macroenvironment elements such as policies that affect unstructured physical activity choices in youth populations. However, the reason for participation has not been fully interpreted from the perspective of the youth themselves, especially those from an Asian cultural background. In our study, we aimed to better understand the self-identified reasons for adolescents’ participation in non-organized or spontaneous tennis practice in contemporary China. Twenty-six adolescents and informants were recruited in mainland China and participated in semi-structured interviews to provide thick descriptions of their continued tennis participation behaviors. Data were coded and analyzed via NVivo 12. Four themes emerged: (a) Individual characteristics and self-interpretations of tennis culture; (b) microsystems mediating adolescents’ tennis participation; (c) barriers and obstacles impacting tennis participation; and (d) policies and macroenvironments. Adolescent tennis participation is a result of the integration effect of the sociocultural and ecological factors dominated by multifaceted ecological systems. As a particular vision of their physical activity experiences, adolescents’ interpretation of tennis and their broader worldview has been continuously reshaped by concurrent sport and educational policies.
Maintaining School Foodservice Operations in Ohio during COVID-19: “This [Was] Not the Time to Sit Back and Watch”
Authors: Braun Ashlea , Hawley Joshua D. , Garner Jennifer A.
Published: May 2022

The COVID-19-related lockdowns led to school closures across the United States, cutting off critical resources for nutritious food. Foodservice employees emerged as frontline workers; understanding their experiences is critical to generate innovations for program operations and viability. The purpose of this cross-sectional study was to characterize COVID-19-related foodservice adaptations for summer and school year meal provision. Public school district foodservice administrators across Ohio were surveyed in December 2020. Questions related to meal provision before, during, and after COVID-19-related school closures. Results indicate the majority of districts continued providing meals upon their closure in Spring 2020 (n = 182, 87.1%); fewer did so in Summer (n = 88, 42.1%) and Fall (n = 32, 15.3%). In Spring and Summer, most districts that offered meals functioned as ‘open sites’ (67.0% and 87.5%, respectively), not limiting food receipt to district-affiliated students. Most districts employed a pick-up system for food distribution (76–84% across seasons), though some used a combination of approaches or changed their approach within-season. Qualitatively, districts reported both “successes” (e.g., supporting students) and “challenges” (e.g., supply chain). Despite being ill-prepared, districts responded quickly and flexibly to demands of the pandemic. This analysis provides insight for future practice (e.g., establishing community partnerships) and policy (e.g., bolstering local food systems).
Few-Shot Building Footprint Shape Classification with Relation Network
Authors: Hu Yaohui , Liu Chun , Li Zheng , Xu Junkui , Han Zhigang , Guo Jianzhong
Published: May 2022

Buildings are important entity objects of cities, and the classification of building shapes plays an indispensable role in the cognition and planning of the urban structure. In recent years, some deep learning methods have been proposed for recognizing the shapes of building footprints in modern electronic maps. Furthermore, their performance depends on enough labeled samples for each class of building footprints. However, it is impractical to label enough samples for each type of building footprint shapes. Therefore, the deep learning methods using few labeled samples are more preferable to recognize and classify the building footprint shapes. In this paper, we propose a relation network based method for the recognization of building footprint shapes with few labeled samples. Relation network, composed of embedding module and relation module, is a metric based few-shot method which aims to learn a generalized metric function and predict the types of the new samples according to their relation with the prototypes of these few labeled samples. To better extract the shape features of the building footprints in the form of vector polygons, we have taken the TriangleConv embedding module to act as the embedding module of the relation network. We validate the effectiveness of our method based on a building footprint dataset with 10 typical shapes and compare it with three classical few-shot learning methods in accuracy. The results show that our method performs better for the classification of building footprint shapes with few labeled samples. For example, the accuracy reached 89.40% for the 2-way 5-shot classification task where there are only two classes of samples in the task and five labeled samples for each class.
Tryptophan in Nutrition and Health
Authors: Poeggeler Burkhard , Singh Sandeep Kumar , Pappolla Miguel A.
Published: May 2022

Tryptophan is a rate-limiting essential amino acid and a unique building block of peptides and proteins [...]
Overexpression of PpSnRK1α in Tomato Increased Autophagy Activity under Low Nutrient Stress
Authors: Luo Jingjing , Yu Wenying , Xiao Yuansong , Zhang Yafei , Peng Futian
Published: May 2022

Plants suffer from a variety of environmental stresses during their growth and development. The evolutionarily conserved sucrose nonfermenting kinase 1-related protein kinase 1 (SnRK1) plays a central role in the regulation of energy homeostasis in response to stresses. In plant cells, autophagy is a degradation process occurring during development or under stress, such as nutrient starvation. In recent years, SnRK1 signaling has been reported to be an upstream activator of autophagy. However, these studies all focused on the regulatory effect of SnRK1 on TOR signaling and the autophagy-related gene 1 (ATG1) complex. In this study, overexpression of the gene encoding the Prunus persica SnRK1 α subunit (PpSnRK1α) in tomato improved the photosynthetic rates and enhanced the resistance to low nutrient stress (LNS). Overexpression of PpSnRK1α increased autophagy activity and upregulated the expression of seven autophagy-related genes (ATGs). The transcriptional levels of SlSnRK2 family genes were altered significantly by PpSnRK1α, signifying that PpSnRK1α may be involved in the ABA signaling pathway. Further analysis showed that PpSnRK1α not only activated autophagy by inhibiting target of rapamycin (TOR) signaling but also enhanced ABA-induced autophagy. This indicates that PpSnRK1α regulates the photosynthetic rate and induces autophagy, and then responds to low nutrient stress.
Identification and Transcriptome Analysis of Genes Related to Membrane Lipid Regulation in Sweet Sorghum under Salt Stress
Authors: Wu Fenghui , Chen Zengting , Zhang Fangning , Zheng Hongxiang , Li Simin , Gao Yinping , Yang Jie , Sui Na
Published: May 2022

Sweet sorghum has strong stress resistance and is considered a promising energy crop. In the present study, the effects of salt on the membrane lipid metabolism of two sweet sorghum inbred lines (salt-tolerant M-81E and salt-sensitive Roma) were analyzed. After treatment with 150 mM NaCl, higher levels of fresh weight and chlorophyll fluorescence, as well as lower levels of malondialdehyde (MDA) were found in salt-tolerant M-81E. Concomitantly, 702 and 1339 differentially expression genes (DEGs) in M-81E and Roma were identified in response to salt stress. We determined that most DEGs were related to glycerophospholipid metabolism, glycerolipid metabolism, and other membrane lipid metabolisms. Under NaCl treatment, the expression of the membrane-associated phospholipase A1 was down-regulated at the transcriptional level, along with an increased content of phosphatidylcholine (PC) in both cultivars. The inhibition of triacylglycerol (TAG) mobilization in M-81E delayed salt-induced leaf senescence. Furthermore, enhanced levels of glycerol-3-phosphate acyltransferase (GPAT) expression contributed to improved salt resistance in M-81E. The results of this study demonstrate membrane the role of lipid regulation in mediating salt-defensive responses in sweet sorghum and expand our understanding of the relationship between changes in membrane lipid content and salt resistance.
Next-Generation Probiotics for Inflammatory Bowel Disease
Authors: Pesce Marcella , Seguella Luisa , Del Re Alessandro , Lu Jie , Palenca Irene , Corpetti Chiara , Rurgo Sara , Sanseverino Walter , Sarnelli Giovanni , Esposito Giuseppe
Published: May 2022

Engineered probiotics represent a cutting-edge therapy in intestinal inflammatory disease (IBD). Genetically modified bacteria have provided a new strategy to release therapeutically operative molecules in the intestine and have grown into promising new therapies for IBD. Current IBD treatments, such as corticosteroids and immunosuppressants, are associated with relevant side effects and a significant proportion of patients are dependent on these therapies, thus exposing them to the risk of relevant long-term side effects. Discovering new and effective therapeutic strategies is a worldwide goal in this research field and engineered probiotics could potentially provide a viable solution. This review aims at describing the proceeding of bacterial engineering and how genetically modified probiotics may represent a promising new biotechnological approach in IBD treatment.
Soyasapogenol C from Fermented Soybean (Glycine Max) Acting as a Novel AMPK/PPARα Dual Activator Ameliorates Hepatic Steatosis: A Novel SANDA Methodology
Authors: Arulkumar Radha , Jung Hee Jin , Noh Sang Gyun , Chung Hae Young
Published: May 2022

(1) Background: Soyasapogenol C (SSC), a derivative of soyasapogenol B (SSB), is specifically found high in many fermented soybean (Glycine max) products, including Cheonggukjang (in Korean). However, the biological activities for preventing and treating hepatic steatosis, and the precise underlying mechanisms of SSC, remain to be explored. (2) Methods: A novel SANDA (structural screening, ADMET prediction, network pharmacology, docking validation, and activity evaluation) methodology was used to examine whether SSC exerts hepatoprotective effects in silico and in vitro. (3) Results: SSC had better ADMET characteristics and a higher binding affinity with predicted targets chosen from network pathway analysis than SSB. SSC induced the phosphorylation of AMP-activated protein kinase (AMPK) and stimulated the nuclear translocation of peroxisome proliferator-activated receptor alpha (PPARα), further enhancing PPAR response element (PPRE) binding activity in HepG2 cells. Concurrently, SSC significantly inhibited triglyceride accumulation, which was associated with the suppression of lipogenesis genes and the enhancement of fatty acid oxidation gene expression in HepG2 cells. (4) Conclusions: Soyasapogenol C, discovered using a novel SANDA methodology from fermented soybean, is a novel AMPK/PPARα dual activator that is effective against hepatic steatosis. Dietary supplementation with soyasapogenol C may prevent the development of hepatic steatosis and other diseases associated with fat accumulation in the liver.
Identification of an Epigenetically Marked Locus within the Sex Determination Region of Channel Catfish
Authors: Yang Yujia , Zhou Tao , Liu Yang , Tian Changxu , Bao Lisui , Wang Wenwen , Zhang Yu , Liu Shikai , Shi Huitong , Tan Suxu , Gao Dongya , Dunham Rex A. , Liu Zhanjiang
Published: May 2022

Channel catfish has an XY sex determination system. However, the X and Y chromosomes harbor an identical gene content of 950 genes each. In this study, we conducted comparative analyses of methylome and transcriptome of genetic males and genetic females before gonadal differentiation to provide insights into the mechanisms of sex determination. Differentially methylated CpG sites (DMCs) were predominantly identified on the sex chromosome, most notably within the sex determination region (SDR), although the overall methylation profiles across the entire genome were similar between genetic males and females. The drastic differences in methylation were located within the SDR at nucleotide position 14.0–20.3 Mb of the sex chromosome, making this region an epigenetically marked locus within the sex determination region. Most of the differentially methylated CpG sites were hypermethylated in females and hypomethylated in males, suggesting potential involvement of methylation modification in sex determination in channel catfish. Along with the differential methylation in the SDR, a number of differentially expressed genes within the SDR were also identified between genetic males and females, making them potential candidate genes for sex determination and differentiation in channel catfish.
Reduced Environmental Dose Rates Are Responsible for the Increased Susceptibility to Radiation-Induced DNA Damage in Larval Neuroblasts of Drosophila Grown inside the LNGS Underground Laboratory
Authors: Porrazzo Antonella , Esposito Giuseppe , Grifoni Daniela , Cenci Giovanni , Morciano Patrizia , Tabocchini Maria Antonella
Published: May 2022

A large amount of evidence from radiobiology studies carried out in Deep Underground Laboratories support the view that environmental radiation may trigger biological mechanisms that enable both simple and complex organisms to cope with genotoxic stress. In line with this, here we show that the reduced radiation background of the LNGS underground laboratory renders Drosophila neuroblasts more sensitive to ionizing radiation-induced (but not to spontaneous) DNA breaks compared to fruit flies kept at the external reference laboratory. Interestingly, we demonstrate that the ionizing radiation sensitivity of flies kept at the LNGS underground laboratory is rescued by increasing the underground gamma dose rate to levels comparable to the low-LET reference one. This finding provides the first direct evidence that the modulation of the DNA damage response in a complex multicellular organism is indeed dependent on the environmental dose rate.
Transformation of Cellulose via Two-Step Carbonization to Conducting Carbonaceous Particles and Their Outstanding Electrorheological Performance
Authors: Plachy Tomas , Kutalkova Erika , Skoda David , Holcapkova Pavlina
Published: May 2022

In this study, cellulose was carbonized in two-steps using hydrothermal and thermal carbonization in sequence, leading to a novel carbonaceous material prepared from a renewable source using a sustainable method without any chemicals and, moreover, giving high yields after a treatment at 600 °C in an inert atmosphere. During this treatment, cellulose was transformed to uniform microspheres with increased specific surface area and, more importantly, conductivity increased by about 7 orders of magnitude. The successful transition of cellulose to conducting carbonaceous microspheres was confirmed through SEM, FTIR, X-ray diffraction and Raman spectroscopy. Prepared samples were further used as a dispersed phase in electrorheological fluids, exhibiting outstanding electrorheological effects with yield stress over 100 Pa at an electric field strength 1.5 kV mm−1 and a particle concentration of only 5 wt%, significantly overcoming recent state-of-the-art findings. Impedance spectroscopy analysis showed clear interfacial polarization of this ER fluid with high dielectric relaxation strength and short relaxation time, which corresponded to increased conductivity of the particles when compared to pure cellulose. These novel carbonaceous particles prepared from renewable cellulose have further potential to be utilized in many other applications that demand conducting carbonaceous structures with high specific surface area (adsorption, catalyst, filtration, energy storage).
CD133-Functionalized Gold Nanoparticles as a Carrier Platform for Telaglenastat (CB-839) against Tumor Stem Cells
Authors: Poonaki Elham , Nickel Ann-Christin , Shafiee Ardestani Mehdi , Rademacher Lars , Kaul Marilyn , Apartsin Evgeny , Meuth Sven G. , Gorji Ali , Janiak Christoph , Kahlert Ulf Dietrich
Published: May 2022

The failure of a long-lasting curative therapeutic benefit of currently applied chemotherapies against malignant cancers is suggested to be caused by the ineffectiveness of such interventions on cancer stem cells (CSCs). CD133/AC133 is a cell surface protein previously shown to have potential to identify CSCs in various tumors, including brain tumors. Moreover, an increase in the rate of cellular metabolism of glutamine and glucose are contributors to the fast cellular proliferation of some high-grade malignancies. Inhibition of glutaminolysis by utilizing pharmacological inhibitors of the enzyme glutaminase 1 (GLS1) can be an effective anti-CSC strategy. In this study, the clinical-stage GLS1 inhibitor Telaglenastat (CB-839) was loaded into PEGylated gold nanoparticles equipped with the covalently conjugated CD133 aptamer (Au-PEG-CD133-CB-839) and exposed to a collection of CD133-positive brain tumor models in vitro. Our results show that Au-PEG-CD133-CB-839 significantly decreased the viability of CD133-postive cancer cells in a dose-dependent manner, which was higher as compared to the effects of treatment of the cells with the individual components of the assembled nanodrug. Interestingly, the treatment effect was observed in glioblastoma stem cells modeling different transcriptomic subtypes of the disease. The presented platform is the fundament for subsequent target specificity characterization and in vivo application.
Molecular Rearrangement of Pyrazino[2,3-c]quinolin-5(6H)-ones during Their Reaction with Isocyanic Acid
Authors: Klásek Antonín , Lyčka Antonín , Křemen Filip , Růžička Aleš , Rouchal Michal
Published: May 2022

New tetrahydropyrazino[2,3-c]quinolin-5(6H)-ones were prepared from 3-chloroquinoline-2,4(1H,3H)-diones and ethylene diamine. In their reaction with HNCO, an unprecedented molecular rearrangement produced new types of hydantoin derivatives. All prepared compounds were characterized on the basis of their 1H, 13C, and 15N NMR and ESI mass spectra and some were authenticated by X-ray analysis of single crystalline material. A proposed mechanism for rearrangement is discussed in this essay. The CDK and ABL inhibition activity as well as in vitro cytotoxicity of the prepared compounds was also tested.
Dopamine Photochemical Behaviour under UV Irradiation
Authors: Falamaş Alexandra , Petran Anca , Hada Alexandru-Milentie , Bende Attila
Published: May 2022

To understand the photochemical behaviour of the polydopamine polymer in detail, one would also need to know the behaviour of its building blocks. The electronic absorption, as well as the fluorescence emission and excitation spectra of the dopamine were experimentally and theoretically investigated considering time-resolved fluorescence spectroscopy and first-principles quantum theory methods. The shape of the experimental absorption spectra obtained for different dopamine species with standard, zwitterionic, protonated, and deprotonated geometries was interpreted by considering the advanced equation-of-motion coupled-cluster theory of DLPNO-STEOM. Dynamical properties such as fluorescence lifetimes or quantum yield were also experimentally investigated and compared with theoretically predicted transition rates based on Fermi’s Golden Rule-like equation. The results show that the photochemical behaviour of dopamine is strongly dependent on the concentration of dopamine, whereas in the case of a high concentration, the zwitterionic form significantly affects the shape of the spectrum. On the other hand, the solvent pH is also a determining factor for the absorption, but especially for the fluorescence spectrum, where at lower pH (5.5), the protonated and, at higher pH (8.0), the deprotonated forms influence the shape of the spectra. Quantum yield measurements showed that, besides the radiative deactivation mechanism characterized by a relatively small QY value, non-radiative deactivation channels are very important in the relaxation process of the electronic excited states of different dopamine species.