bluuebunny/crossref_metadata_2025 huggingface dataset:
A Prognostic Model Using Post-Steroid Neutrophil-Lymphocyte Ratio Predicts Overall Survival in Primary Central Nervous System Lymphoma
Authors: Lo Yu Tung , Lim Vivian Yujing , Ng Melissa , Tan Ya Hwee , Chiang Jianbang , Chang Esther Wei Yin , Chan Jason Yongsheng , Poon Eileen Yi Ling , Somasundaram Nagavalli , Bin Harunal Rashid Mohamad Farid , Tao Miriam , Lim Soon Thye , Yang Valerie Shiwen
Published: April 2022

Background: Ratios of differential blood counts (hematological indices, HIs) had been identified as prognostic variables in various cancers. In primary central nervous system lymphomas (PCNSLs), higher baseline neutrophil-lymphocyte ratio (NLR) in particular was found to portend a worse overall survival. However, it was often observed that differential counts shift drastically following steroid administration. Moreover, steroids are an important part of the arsenal against PCNSL due to its potent lymphotoxic effects. We showed that the effect of steroids on differential blood cell counts and HIs could be an early biomarker for subsequent progression-free (PFS) and overall survival (OS). Methods: This study retrospectively identified all adult patients who received a brain biopsy from 2008 to 2019 and had histologically confirmed PCNSL, and included only those who received chemoimmunotherapy, with documented use of corticosteroids prior to treatment induction. Different blood cell counts and HIs were calculated at three time-points: baseline (pre steroid), pre chemoimmunotherapy (post steroid) and post chemoimmunotherapy. Tumor progression and survival data were collected and analyzed through Kaplan–Meier estimates and Cox regression. We then utilized selected variables found to be significant on Kaplan–Meier analysis to generate a decision-tree prognostic model, the NNI-NCCS score. Results: A total of 75 patients who received chemoimmunotherapy were included in the final analysis. For NLR, OS was longer with higher pre-chemoimmunotherapy (post-steroid) NLR (dichotomized at NLR ≥ 4.0, HR 0.42, 95% CI: 0.21–0.83, p = 0.01) only. For platelet-lymphocyte ratio (PLR) and lymphocyte-monocyte ratio (LMR), OS was better for lower post-chemoimmunotherapy PLR (dichotomized at PLR ≥ 241, HR 2.27, 95% CI: 1.00 to 5.18, p = 0.05) and lower pre-chemoimmunotherapy (post-steroid) LMR (dichotomized at LMR ≥25.7, HR 2.17, 95% CI: 1.10 to 4.31, p = 0.03), respectively, only. The decision-tree model using age ≤70, post-steroid NLR >4.0, and pre-steroid (baseline) NLR <2.5 and the division of patients into three risk profiles—low, medium, and high—achieved good accuracy (area-under-curve of 0.78), with good calibration (Brier score: 0.16) for predicting 2-year overall survival. Conclusion: We found that post-steroid NLR, when considered together with baseline NLR, has prognostic value, and incorporation into a prognostic model allowed for accurate and well-calibrated stratification into three risk groups.
Oxidative Stress-Induced TRPV2 Expression Increase Is Involved in Diabetic Cataracts and Apoptosis of Lens Epithelial Cells in a High-Glucose Environment
Authors: Chen Linghui , Chen Yanzhuo , Ding Wen , Zhan Tao , Zhu Jie , Zhang Lesha , Wang Han , Shen Bing , Wang Yong
Published: April 2022

Cataracts are a serious complication of diabetes. In long-term hyperglycemia, intracellular Ca2+ concentration ([Ca2+]i) and reactive oxygen species (ROS) are increased. The apoptosis of lens epithelial cells plays a key role in the development of cataract. We investigated a potential role for transient receptor potential vanilloid 2 (TRPV2) in the development of diabetic cataracts. Immunohistochemical and Western blotting analyses showed that TRPV2 expression levels were significantly increased in the lens epithelial cells of patients with diabetic cataracts as compared with senile cataract, as well as in both a human lens epithelial cell line (HLEpiC) and primary rat lens epithelial cells (RLEpiCs) cultured under high-glucose conditions. The [Ca2+]i increase evoked by a TRPV2 channel agonist was significantly enhanced in both HLEpiCs and RLEpiCs cultured in high-glucose media. This enhancement was blocked by the TRPV2 nonspecific inhibitor ruthenium red and by TRPV2-specific small interfering (si)RNA transfection. Culturing HLEpiCs or RLEpiCs for seven days in high glucose significantly increased apoptosis, which was inhibited by TRPV2-specific siRNA transfection. In addition, ROS inhibitor significantly suppressed the ROS-induced increase of TRPV2-mediated Ca2+ signal and apoptosis under high-glucose conditions. These findings suggest a mechanism underlying high-glucose–induced apoptosis of lens epithelial cells, and offer a potential target for developing new therapeutic options for diabetes-related cataracts.
The Paracrine Effect of Hypoxic and Normoxic Cancer Secretion on the Proliferation of Brain Endothelial Cells (bEnd.3)
Authors: Rado Mariam , Fisher David
Published: April 2022

Background: This study aimed to investigate the disruption of cell cycle phases of bEnd.3 cells exposed to cancer paracrine secretion. Cancer cells have been reported to use the secretion of paracrine factors to compromise the endothelial barrier to prepare for their passage into the parenchyma. As cancer cells are known to act differently under conditions of hypoxia, we investigated how conditional media (CM) derived from breast and glioblastoma cells incubated under conditions of normoxia and hypoxia would affect proliferation of brain endothelial cells (bEnd.3). Methods: Brain endothelial cells (bEnd.3) were cultivated with normoxic and hypoxic CM generated from breast cancer MCF7 cells and glioblastoma U-87 cells. Cell proliferation was evaluated using the trypan blue exclusion assay and phases of the cell cycle were evaluated using flow cytometry. Results: bEnd.3 proliferations was suppressed more aggressively with hypoxic CM after 72 and 96 h; cell cycle analysis showed that paracrine treatment tended to prevent BECs from entering the G2 phase, thus suppressing cell division. Conclusions: MCF7 and U-87 cells induce suppressed proliferation of BECs deferentially under hypoxia by blocking cell cycle progression to the G2 phase.
Effects of Adipose-Derived Stem Cells and Their Conditioned Medium in a Human Ex Vivo Wound Model
Authors: Guo Xiao , Schaudinn Christoph , Blume-Peytavi Ulrike , Vogt Annika , Rancan Fiorenza
Published: April 2022

Adult stem cells have been extensively investigated for tissue repair therapies. Adipose-derived stem cells (ASCs) were shown to improve wound healing by promoting re-epithelialization and vascularization as well as modulating the inflammatory immune response. In this study, we used ex vivo human skin cultured in a six-well plate with trans-well inserts as a model for superficial wounds. Standardized wounds were created and treated with allogeneic ASCs, ASCs conditioned medium (ASC-CM), or cell culture medium (DMEM) supplemented with fetal calf serum (FCS). Skin viability (XTT test), histology (hematoxylin and eosin, H and E), β-catenin expression as well as inflammatory mediators and growth factors were monitored over 12 days of skin culture. We observed only a moderate time-dependent decrease in skin metabolic activity while skin morphology was preserved, and re-epithelialization occurred at the wound edges. An increase in β-catenin expression was observed in the newly formed epithelia, especially in the samples treated with ASC-CM. In general, increased growth factors and inflammatory mediators, e.g., hepatocytes growth factor (HGF), platelet-derived growth factor subunit AA (PDGF-AA), IL-1α, IL-7, TNF-α, and IL-10, were observed over the incubation time. Interestingly, different expression profiles were observed for the different treatments. Samples treated with ASC-CM significantly increased the levels of inflammatory cytokines and PDGF-AA with respect to control, whereas the treatment with ASCs in DMEM with 10% FCS resulted in significantly increased levels of fibroblast growth factor-basic (FGF-basic) and moderate increases of immunomodulatory cytokines. These results confirm that the wound microenvironment can influence the type of mediators secreted by ASCs and the mode as to how they improve the wound healing process. Comparative investigations with pre-activated ASCs will elucidate further aspects of the wound healing mechanism and improve the protocols of ACS application.
TGF-β1 Reduces Neutrophil Adhesion and Prevents Acute Vaso-Occlusive Processes in Sickle Cell Disease Mice
Authors: Torres Lidiane , Chweih Hanan , Fabris Fernanda , Gotardo Erica , Leonardo Flávia , Saad Sara , Costa Fernando , Conran Nicola
Published: April 2022

Sickle cell disease (SCD) patients experience chronic inflammation and recurrent vaso-occlusive episodes during their entire lifetime. Inflammation in SCD occurs with the overexpression of several inflammatory mediators, including transforming growth factor beta-1 (TGF-β1), a major immune regulator. In this study, we aimed to investigate the role played by TGF-β1 in vascular inflammation and vaso-occlusion in an animal model of SCD. Using intravital microscopy, we found that a daily dose of recombinant TGF-β1 administration for three consecutive days significantly reduced TNFα-induced leukocyte rolling, adhesion, and extravasation in the microcirculation of SCD mice. In contrast, immunological neutralization of TGF-β, in the absence of inflammatory stimulus, considerably increased these parameters. Our results indicate, for the first time, that TGF-β1 may play a significant ameliorative role in vascular SCD pathophysiology, modulating inflammation and vaso-occlusion. The mechanisms by which TGF-β1 exerts its anti-inflammatory effects in SCD, however, remains unclear. Our in vitro adhesion assays with TNFα-stimulated human neutrophils suggest that TGF-β1 can reduce the adhesive properties of these cells; however, direct effects of TGF-β1 on the endothelium cannot be ruled out. Further investigation of the wide range of the complex biology of this cytokine in SCD pathophysiology and its potential therapeutical use is needed.
The Inhibitory Effect of Selected D2 Dopaminergic Receptor Agonists on VEGF-Dependent Neovascularization in Zebrafish Larvae: Potential New Therapy in Ophthalmic Diseases
Authors: Kasica Natalia , Święch Anna , Saładziak Katarzyna , Mackiewicz Jerzy , Osęka Maciej
Published: April 2022

Pathological angiogenesis is correlated with many ophthalmic diseases. The most common are exudative age-related macular degeneration and proliferative diabetic retinopathy. The current treatment for these diseases is based on regularly administered anti-VEGF antibodies injections. In the study, we investigated selected D2 dopaminergic receptor agonists, namely bromocriptine, cabergoline and pergolide, on hypoxia-induced neovascularization. We used the zebrafish laboratory model, specifically three-day post fertilization (dpf) Tg(fli-1: EGFP) zebrafish larvae. To induce abnormal angiogenesis of hyaloid-retinal vessels (HRVs) and intersegmental vessels (ISVs), the larvae were treated with cobalt chloride (II) (CoCl2) (a hypoxia-inducing agent) from 24 h post fertilization. The inhibitory role of D2 dopaminergic receptor agonists was investigated using confocal microscopy and qPCR. Additionally, the results were compared to those obtained in the group treated with CoCl2 followed by bevacizumab, the well-known antiangiogenic agent. Confocal microscopy analyses revealed severe deformation of vessels in the CoCl2 treated group, while co-incubation with bromocriptine, cabergoline, pergolide and bevacizumab, respectively, significantly inhibited abnormalities of angiogenesis. The qPCR analyses supported the protective role of the chosen dopaminergic agonists by demonstrating their influence on CoCl2-derived upregulation of vegfaa expression. The present results suggest that the D2 receptor agonists can be considered as a new direction in research for antiangiogenic therapy.
The Interplay between Autophagy and Redox Signaling in Cardiovascular Diseases
Authors: Boťanská Barbora , Dovinová Ima , Barančík Miroslav
Published: April 2022

Reactive oxygen and nitrogen species produced at low levels under normal cellular metabolism act as important signal molecules. However, at increased production, they cause damage associated with oxidative stress, which can lead to the development of many diseases, such as cardiovascular, metabolic, neurodegenerative, diabetes, and cancer. The defense systems used to maintain normal redox homeostasis plays an important role in cellular responses to oxidative stress. The key players here are Nrf2-regulated redox signaling and autophagy. A tight interface has been described between these two processes under stress conditions and their role in oxidative stress-induced diseases progression. In this review, we focus on the role of Nrf2 as a key player in redox regulation in cell response to oxidative stress. We also summarize the current knowledge about the autophagy regulation and the role of redox signaling in this process. In line with the focus of our review, we describe in more detail information about the interplay between Nrf2 and autophagy pathways in myocardium and the role of these processes in cardiovascular disease development.
Chaperone-Mediated Autophagy in Neurodegenerative Diseases and Acute Neurological Insults in the Central Nervous System
Authors: Kanno Haruo , Handa Kyoichi , Murakami Taishi , Aizawa Toshimi , Ozawa Hiroshi
Published: April 2022

Autophagy is an important function that mediates the degradation of intracellular proteins and organelles. Chaperone-mediated autophagy (CMA) degrades selected proteins and has a crucial role in cellular proteostasis under various physiological and pathological conditions. CMA dysfunction leads to the accumulation of toxic protein aggregates in the central nervous system (CNS) and is involved in the pathogenic process of neurodegenerative diseases, including Parkinson’s disease and Alzheimer’s disease. Previous studies have suggested that the activation of CMA to degrade aberrant proteins can provide a neuroprotective effect in the CNS. Recent studies have shown that CMA activity is upregulated in damaged neural tissue following acute neurological insults, such as cerebral infarction, traumatic brain injury, and spinal cord injury. It has been also suggested that various protein degradation mechanisms are important for removing toxic aberrant proteins associated with secondary damage after acute neurological insults in the CNS. Therefore, enhancing the CMA pathway may induce neuroprotective effects not only in neurogenerative diseases but also in acute neurological insults. We herein review current knowledge concerning the biological mechanisms involved in CMA and highlight the role of CMA in neurodegenerative diseases and acute neurological insults. We also discuss the possibility of developing CMA-targeted therapeutic strategies for effective treatments.
Application of Liquid Waste from Biogas Production for Microalgae Chlorella sp. Cultivation
Authors: Sendzikiene Egle , Makareviciene Violeta
Published: April 2022

Microalgae biomass is a viable feedstock for a wide range of industries. Recently, there has also been interest in the ability of microalgae biomass applications for biofuel production. In the meantime, the cultivation of microalgae biomass requires high energy costs, and the application of microalgae for technical purposes is still problematic. A significant part of the cost of biomass arises from the nutrients used for cultivation. Chemical compounds included in the microalgae cultivation media can be replaced by suitable wastes containing nitrogen, phosphorus, and other elements. This could reduce the microalgae biomass cultivation price and allow cheaper biomass to be used for biofuel production. The aim of this work was to comprehensively investigate and optimize the growth process of microalgae using liquid waste (liquid waste after biogas production from sewage sludge and distillers’ grain) as a source of nitrogen and phosphorus, and technical glycerol as a carbon source. It was found that higher levels of waste in the cultivation media were found to inhibit the accumulation of microalgal biomass, with the optimum level corresponding to a nitrogen concentration of 0.08 g/L. The influence of technical glycerol from biodiesel production on the yield of microalgal biomass was investigated, and it was found that the addition of 6% glycerol allows an increase in the concentration of microalgal biomass in the cultivation media, from 18.1 to 20.6%.
The Role of Skeletal Muscle in Neuromuscular Diseases: From Cellular and Molecular Players to Therapeutic Interventions
Authors: Dobrowolny Gabriella , Scicchitano Bianca Maria
Published: April 2022

Genetic and acquired defects of lower motor neurons, peripheral nerves, or skeletal muscle are responsible for several neuromuscular disorders [...]
Extracellular Lipids in the Lung and Their Role in Pulmonary Fibrosis
Authors: Burgy Olivier , Loriod Sabrina , Beltramo Guillaume , Bonniaud Philippe
Published: April 2022

Lipids are major actors and regulators of physiological processes within the lung. Initial research has described their critical role in tissue homeostasis and in orchestrating cellular communication to allow respiration. Over the past decades, a growing body of research has also emphasized how lipids and their metabolism may be altered, contributing to the development and progression of chronic lung diseases such as pulmonary fibrosis. In this review, we first describe the current working model of the mechanisms of lung fibrogenesis before introducing lipids and their cellular metabolism. We then summarize the evidence of altered lipid homeostasis during pulmonary fibrosis, focusing on their extracellular forms. Finally, we highlight how lipid targeting may open avenues to develop therapeutic options for patients with lung fibrosis.
Age and Gender Are Associated with the Component of Psychosocial Impact of Dental Aesthetics Questionnaire in Young People: A Cross-Sectional Study
Authors: Wan Hassan Wan Nurazreena , Makhbul Mohd Zambri Mohamed , Othman Siti Adibah
Published: April 2022

(1) The aim of the study was to investigate the association between age, gender, and the component of psychosocial impact of dental aesthetics (PIDAQ) in Malaysian young people. (2) Cross-sectional data on the PIDAQ (comprised of dental self-confidence, social impact, psychological impact, and aesthetic concern variables) of Malaysian youth (n = 1425) recruited through multi-stage sampling were analyzed for mediation and moderated mediation analyses using the PROCESS macro on SPSS software. (3) Participants (mean age 16.0 ± 2.8) represented 54.8% of girls and 45.2% of boys. In the mediation model, psychological impact and aesthetic concern completely mediated the effects of social impact on dental self-confidence. In the moderated mediation model, social impact directly influenced dental self-confidence amongst participants at one standard deviation below the sample mean age and among boys. However, psychological impact completely mediated the influence of social impact on dental self-confidence amongst participants at the sample mean age and at one standard deviation above the sample mean ages, and among girls. Neither age nor gender moderated the effect of aesthetic concern on dental self-confidence. (4) Age and gender moderated the influence of social impact and psychological impact on dental self-confidence.
Transcatheter Device Therapy and the Integration of Advanced Imaging in Congenital Heart Disease
Authors: Divekar Abhay A. , Arar Yousef M. , Clark Stephen , Tandon Animesh , Zellers Thomas M. , Veeram Reddy Surendranath R.
Published: April 2022

Transcatheter device intervention is now offered as first line therapy for many congenital heart defects (CHD) which were traditionally treated with cardiac surgery. While off-label use of devices is common and appropriate, a growing number of devices are now specifically designed and approved for use in CHD. Advanced imaging is now an integral part of interventional procedures including pre-procedure planning, intra-procedural guidance, and post-procedure monitoring. There is robust societal and industrial support for research and development of CHD-specific devices, and the regulatory framework at the national and international level is patient friendly. It is against this backdrop that we review transcatheter implantable devices for CHD, the role and integration of advanced imaging, and explore the current regulatory framework for device approval.
Essential Management of Pediatric Brain Tumors
Authors: Lutz Katharina , Jünger Stephanie T. , Messing-Jünger Martina
Published: April 2022

Brain tumors are the most common solid tumors in children and are associated with high mortality. The most common childhood brain tumors are grouped as low-grade gliomas (LGG), high grade gliomas (HGG), ependymomas, and embryonal tumors, according to the World Health Organization (WHO). Advances in molecular genetics have led to a shift from pure histopathological diagnosis to integrated diagnosis. For the first time, these new criteria were included in the WHO classification published in 2016 and has been further updated in the 2021 edition. Integrated diagnosis is based on molecular genomic similarities of the tumor subclasses, and it can better explain the differences in clinical courses of previously histopathologically identical entities. Important advances have also been made in pediatric neuro-oncology. A growing understanding of the molecular-genetic background of tumorigenesis has improved the diagnostic accuracy. Re-stratification of treatment protocols and the development of targeted therapies will significantly affect overall survival and quality of life. For some pediatric tumors, these advances have significantly improved therapeutic management and prognosis in certain tumor subgroups. Some therapeutic approaches also have serious long-term consequences. Therefore, optimized treatments are greatly needed. Here, we discuss the importance of multidisciplinary collaboration and the role of (pediatric) neurosurgery by briefly describing the most common childhood brain tumors and their currently recognized molecular subgroups.
The Needs of Adolescents and Young Adults with Chronic Illness: Results of a Quality Improvement Survey
Authors: Allen Taryn , Reda Stephanie , Martin Staci , Long Phoebe , Franklin Alexis , Bedoya Sima Zadeh , Wiener Lori , Wolters Pamela L.
Published: April 2022

Adolescent and young adults (AYAs) with chronic illnesses cope with complex issues that require unique psychological support and healthcare services to reduce psychosocial difficulties, improve disease management, and facilitate positive transitions to adult care. Engaging patients and caregivers can help providers understand the specific needs of this population and identify the perceived areas of support. The purpose of this quality improvement initiative is to assess the needs of AYAs with chronic medical conditions at a large government research hospital. Eighty-nine AYA patients (age = 23.5 years; range 13–34) with neurofibromatosis type 1, cancer, primary immunodeficiencies, or sickle cell disease, and a sample of caregivers (n = 37, age = 52 years; range: 41–65), completed an anonymized survey that assessed their preferences for a wide range of informational and service-related needs. The results indicate an overwhelming desire for information about general health and wellbeing and disease-specific medical knowledge. The most endorsed item was the need for more information about an individual’s medical condition (72%), which was a primary concern across disease, racial, and gender groups. Demographic and disease-specific needs were also identified. Thus, providing information to AYA patients and caregivers is a critical and largely unmet component of care, which requires the development and implementation of targeted educational and psychosocial interventions.
Transitional Care in Pediatric Brain Tumor Patients: A Systematic Literature Review
Authors: Ebel Florian , Greuter Ladina , Guzman Raphael , Soleman Jehuda
Published: April 2022

Background: Due to advances in the treatment of pediatric brain tumors (PBT), an increasing number of patients are experiencing the transition from the pediatric to the adult health care system. This requires efficient transitional models. Methods: We systematically reviewed the literature regarding PBT concerning different transitional models and aspects of the transitional period. For this purpose, PubMed, Medline, and Embase databases were searched systematically through January 2022. Results: We reviewed a total of 304 studies, of which 15 were ultimately included. We identified five transition models described within the literature, while the most frequently mentioned ones were the “adult caregiver model” (45.5%), “joint caregiver model” (45.5%), “continued caregiver model” (27.3%), and the “specialized clinic model” (27.3%). During the transition, the most frequent challenges mentioned by the patients were the lack of knowledge about the disease by the adult health care professionals (62.5%) and the difficulty of establishing a new relationship with the new physician, environment, or hospital (37.5%). Conclusions: An efficient transitional model is mandatory for patients with PBT. Continuity in the treatment and care of the patient and their family is essential. For this purpose, in patients with PBT, the “continued caregiver model”, and for NF1 and TSC patients, the “specialized clinic model” seems optimal to offer continuity of care. If such models are unavailable, efficient communication with patients, families, and specialists in a multidisciplinary network is even more critical.
Leukapheresis in Pediatric Acute Leukemia with Hyperleukocytosis: A Single-Center Experience
Authors: Jones Sandra Renee , Rahrig April , Saraf Amanda J.
Published: April 2022

Hyperleukocytosis in pediatric acute leukemia is associated with increased morbidity and mortality and at present there is no consensus on the use of leukapheresis (LPH) for its management. Our aim was to review characteristics and outcomes of newly diagnosed leukemia patients with hyperleukocytosis (HL) comparing those who received LPH and those who did not. An IRB approved retrospective case control study reviewed data from a single institution over a 10 year period. At our institution, LPH was used in 8 of 62 (13%) patients with hyperleukocytosis with minimal complications. Mean leukocyte count in patients who received LPH versus those who did not was 498 k cells/mm3 and 237 k cells/mm3, respectively. Patients who had symptoms of neurologic (63 vs. 17%) or pulmonary leukostasis (75 vs. 17%) were more likely to have undergone leukapheresis. The time from presentation to the initiation of chemotherapy was not different between those who received LPH and those who did not (mean of 35 h vs. 34 h). There was one death in the LPH group, that was the result of neurologic sequelae of hyperleukocytosis and not LPH itself. The use of LPH in patients with hyperleukocytosis is safe, well tolerated and does not alter time to chemotherapy at our institution.
Association between Stress Hyperglycemia and Adverse Outcomes in Children Visiting the Pediatric Emergency Department
Authors: Bae Woori , Ahn Moon Bae
Published: April 2022

Stress hyperglycemia (SH) is often identified in patients visiting the pediatric emergency department (PED), and SH in adults has been associated with adverse outcomes, including mortality. In this retrospective study, we determined the adverse outcomes according to blood glucose (BG) levels of children visiting the PED of tertiary hospitals. Data were collected from the electronic medical records of children aged <18 years between 1 January 2011 and 31 December 2020. A total of 44,905 visits were included in the analysis. SH was identified in 1506 patients, with an incidence rate of 3.4%. Compared to those without SH, patients with SH had significantly higher ward admission rates (52.6% vs. 35.9%, p < 0.001), intensive care unit admission rates (2.6% vs. 0.7%, p < 0.001), and mortality rates (2.7% vs. 0.3%, p < 0.001). Compared to the normoglycemic group of 45 ≤ BG < 150 mg/dL, the odds ratios (95% CI) for mortality were 5.61 (3.35–9.37), 27.96 (14.95–52.26), 44.22 (17.03–114.82), and 39.94 (16.31–97.81) for levels 150 ≤ BG < 200, 200 ≤ BG < 250, 250 ≤ BG < 300 and ≥300 mg/dL, respectively. This suggests that SH is common in children visiting the PED and is associated with higher adverse outcomes. Thus, there is a need to quickly identify its cause and take prompt intervention to resolve it.
Executive Function Improvement for Children with Autism Spectrum Disorder: A Comparative Study between Virtual Training and Physical Exercise Methods
Authors: Ji Chaoxin , Yang Jun , Lin Lin , Chen Song
Published: April 2022

This study evaluated and compared the effects of virtual training and physical exercise on the executive function of children with autism spectrum disorder (ASD). After screening, the final analysis of this study was conducted on three groups: a virtual training group (n = 34), a physical exercise group (n = 33), and a control group (n = 33). The experiment was conducted for nine weeks, of which the virtual training group and physical exercise group were conducted three times a week for one hour each time during the first six weeks, while the control group did not conduct virtual training nor physical exercise. During the last three weeks (week 6 to week 9), virtual training and physical exercise were not performed on all three groups. The three main components of executive function (working memory, inhibition, flexibility) of children with ASD were measured before the intervention, after the intervention (week 1 to week 6) and in the last three weeks (week 6 to week 9). The final results are that firstly, the executive function of the virtual training and physical exercise groups were simultaneously improved after the intervention. Secondly, after the intervention stopped, the executive function of the virtual training and physical exercise groups showed a downward trend. Therefore, the study concludes that the application of virtual training and physical exercise can effectively enhance the executive function of children with ASD.
Early Childhood Temperamental Trajectories following Very Preterm Birth and Their Association with Parenting Style
Authors: Lovato Irene , Vanes Lucy D. , Sacchi Chiara , Simonelli Alessandra , Hadaya Laila , Kanel Dana , Falconer Shona , Counsell Serena , Redshaw Maggie , Kennea Nigel , Edwards Anthony David , Nosarti Chiara
Published: April 2022

Childhood temperament is an early characteristic shaping later life adjustment. However, little is currently known about the stability of early temperament and its susceptibility to the environment in children born very preterm (VPT; <33 weeks’ gestation). Here, we investigated infant-to-childhood temperamental trajectories, and their interaction with parental practices, in VPT children. Maternal reports of infant temperament were collected in 190 infants (mean age: 11.27 months; range 9–18 months) enrolled in the longitudinal Evaluation of Preterm Imaging (ePrime; Eudra: CT 2009-011602-42) study, using the ePrime questionnaire on infant temperament. At 4–7 years of age, further assessments of child temperament (Children’s Behavior Questionnaire—Very Short Form) and parenting style (Arnold’s Parenting Scale) were conducted. Results showed that more difficult temperament in infancy was associated with increased Negative Affectivity in childhood, regardless of parenting practices. This lends support to the stability of early temperamental traits reflecting negative emotionality. In contrast, a lax parenting style moderated the relationship between easy infant temperament and Negative Affectivity at 4–7 years, such that an easier infant temperament was increasingly associated with higher childhood Negative Affectivity scores as parental laxness increased. These results highlight a potential vulnerability of VPT infants considered by their mothers to be easy to handle, as they may be more susceptible to the effects of suboptimal parenting in childhood.
Ultrasonographic Assessment for Tenosynovitis in Juvenile Idiopathic Arthritis with Ankle Involvement: Diagnostic and Therapeutic Significance
Authors: Della Paolera Sara , Pastore Serena , Zabotti Alen , Tommasini Alberto , Taddio Andrea
Published: April 2022

Background: The role of musculoskeletal ultrasound in JIA is still controversial, although there is growing evidence on its utility, especially in the diagnosis of tenosynovitis. Methods: We presented a retrospective cross-sectional study of a group of patients with JIA with ankle swelling followed in a Pediatric Rheumatology Service of a tertiary-level pediatric hospital in Northern Italy during the follow-up period between January 1st 2003 and December 31st 2019. Preliminary results have been presented at the EULAR Congress 2021. We enrolled only patients who underwent msk-US, and we identified those with a clinical and sonographic diagnosis of tenosynovitis. For each patient, we collected data on demographics, clinical characteristics, and therapeutic strategies during the follow-up. Results: On December 31st 2019, 56 swollen ankles of 48 patients were assessed with msk-US. Twenty-two ankles showed sonographic signs of joint synovitis, sixteen ankles presented signs of both joint synovitis and tenosynovitis, and fourteen ankles presented sonographic signs of tenosynovitis only. Overall, tenosynovitis was detected on 27 (56%) out of 48 children with at least a swollen ankle. In 13 patients out of 27 with tenosynovitis (48%), there was no joint synovitis of ankle or foot. Twenty-five patients with tenosynovitis (92%) achieved clinical and radiological remission: seven patients achieved remission of tenosynovitis with methotrexate only, and fifteen patients with biological drugs alone or in combination therapy. Conclusions: We observed that more than half of the patients with ankle swelling presented a tenosynovitis, and about 50% of them did not show sonographic signs of an active joint synovitis. Among patients with tenosynovitis, biological therapy alone or in association with DMARDs showed effectiveness in inducing disease remission.
Climate Change Effects on Heating and Cooling Demands of Buildings in Canada
Authors: Chidiac Samir E. , Yao Lan , Liu Paris
Published: April 2022

Climate change is causing more frequent extreme weather events. The consequences of increasing global temperature on the operating cost of existing buildings, and the associated health, safety, and economic risks were investigated. Eight cities in Ontario, Canada, across climate zones 5 to 8, were selected for this study. Statistical models were employed to forecast daily temperatures for 50 years. The impact of climate change on buildings’ heating and cooling demands for energy was measured as changes in heating degree days (HDD) and cooling degree days (CDD) compared to current design requirements. The results predict an increase in the demand for cooling and a decrease in that for heating within the next 50 years. A drop in the total HDD and CDD is shown which reflects a more comfortable outdoor thermal condition. Risk to human health attributable to the increase in global temperature is negligible.
Investigating the Water Jet Erosion Performance of HVOF-Sprayed WC-10Co Coatings on 35CrMo Steel Utilizing Design of Experiments
Authors: Ribu Daniel C. , Rajesh Rajamony , Thirumalaikumarasamy Duraisamy , Ramachandran Chidambaram Seshadri , Ahamed Saleel C. , Aabid Abdul , Baig Muneer , Saleh Bahaa
Published: April 2022

To enhance the surface of a material with the desired qualities for diverse applications in service, a variety of thermal and thermo-chemical surface treatment processes are used. Due to the high-velocity impact inherent in the process, high-velocity oxy-fuel (HVOF) spray is now frequently employed in industrial applications for its ability to generate a high-quality coating with appropriate hardness and low oxide content. In this investigation, a high-velocity oxy-fuel (HVOF) thermal spraying process was utilized to coat WC-10Co powders on a 35CrMo steel substrate. A water jet erosion test was also used to examine the substrate and coated samples’ erosion behavior. The erosion rate was systematically investigated using water jet variables such as the angle of impingement, water jet velocity, standoff distance, and erodent discharge. For the development of multiple regression models, experiments were performed utilizing the central composite rotatable design and the response surface methodology. The angle of impingement had the most impact on the rate of coating erosion, leading to the water jet velocity, standoff distance, and erodent discharge.
Measurement of Multi-Stokes Ultrashort Pulse Shapes of Synchronously Pumped Stimulated Raman Scattering on Combined Vibrational Modes in a BaWO4 Crystal
Authors: Tereshchenko Dmitry P. , Peganov Egor A. , Smetanin Sergei N. , Papashvili Alexander G. , Shashkov Evgeny V. , Ivleva Lyudmila I. , Dunaeva Elizaveta E. , Voronina Irina S. , Frank Milan
Published: April 2022

Multi-Stokes ultrashort pulse shapes and their relative positions of synchronously pumped stimulated Raman scattering (SRS) on combined primary and secondary vibrational modes in a BaWO4 crystal are investigated. An original method of its simultaneous measurement with the help of a streak camera has been developed. The structure of SRS pulses at the pulse shortening effect down to the pulse duration, close to the dephasing time of the secondary Raman mode of the BaWO4 crystal, is registered and analyzed for the detuning of the Raman laser cavity length.
Thermal and Electron Plasma Effects on Phase Separation Dynamics Induced by Ultrashort Laser Pulses
Authors: Cavillon Maxime , Cao Jing , Vallet Maxime , Brisset François , Mazerolles Léo , Dkhil Brahim , Lancry Matthieu , Poumellec Bertrand
Published: April 2022

During ultrafast laser-induced crystallization from glass with a non-congruent composition, a phase separation occurs. The morphology of the crystallized area, inside the heat-affected zone (HAZ), is spectacular showing a bouquet-like structure, under some specific conditions related to glass chemical composition and laser parameters. In this work, we investigate this HAZ along a written line through a set of high-resolution electron microscopy techniques to probe both the morphology and the chemical distribution at the nanoscale. Based on these findings, we demonstrate that the bouquet-like structure arises from poorly textured nanocrystals between two regions that have probably accumulated elastic strain. From that analysis, we also provide insights into the chemical separation process during this complex light-matter transformation in which the induced plasma structure guides the spatial distribution of SiO2 and LiNbO3. We suggest a model based on an electric field modulation produced by the inhomogeneous plasma electron trapping, that modifies the electrochemical potentials of the constituents.
Linking Intermediate to Final “Real-World” Outcomes: Is Financial Toxicity a Reliable Predictor of Poorer Outcomes in Cancer?
Authors: Longo Christopher J.
Published: April 2022

Traditionally, economic evaluations are based on clinical trials with well-defined patient populations that exclude many patient types. By contrast, studies that incorporate general patient populations end up including those in lower income categories, some of whom have significant financial burdens (often described as financial toxicity) related to their care. Consideration of these patient burdens when examining the incremental cost-effectiveness of newer treatments from a clinical trial perspective can result in differing conclusions regarding cost-effectiveness. The challenge is to reliably assess the link between financial toxicity, quality of life and potential decisions to forego or delay care. It is also well-documented that these financial effects are not evenly distributed across populations, with those with low income and of black or Latino decent being most affected. There is a paucity of literature in this space, but some of the early work has suggested that for lung, breast, colorectal and ovarian cancers there are poorer quality-of-life scores and/or shorter overall survival for those experiencing financial toxicity. Hence, we may see both a lower quality of life and a shorter duration of life for these populations. If this is the case, additional considerations include: are the benefits of newer, more-expensive treatment strategies muted by the lack of adherence to these newer treatments due to financial concerns, and, if true, can these effects be effectively quantified as “real-world” outcomes? This rapid review examines these possibilities and the steps that may be required to examine this reliably.
Access Denied? The Unintended Consequences of Pending Drug Pricing Rules
Authors: Kaplan Alan , Stewart David J. , Batist Gerald , Spadafora Silvana , Sehdev Sandeep , Goodman Shaun G.
Published: April 2022

The government of Canada now plans to bring into force new federal drug pricing regulations on 1 July 2022. We do not take issue with the goal of medication affordability, which is vital in healthcare the world over. Our concern is that the new guidelines are being implemented without due consideration for three major unintended consequences: regulatory changes will lower the number of clinical trials for new medications in Canada, fewer clinical trials will mean lower research and development investments, and changes will reduce patients’ access to new medications. Access to effective medications is a cornerstone of healthcare for Canadian patients. As physicians, our duty to patient care demands that we tell the government to protect the right of Canadians to timely access to life-changing medicines.
Comparative Evaluation of Free Web Tools ImageJ and Photopea for the Surface Area Quantification of Planar Substrates and Organisms
Authors: El-Khaled Yusuf C. , Kler Lago Alexandra , Mezger Selma D. , Wild Christian
Published: April 2022

Biological imaging is an essential tool to visualise and obtain reference data. In this context, the programme ImageJ has been widely used in many disciplines to determine the surface areas of planar biological samples in marine and aquatic experimental biology. Despite its range of advantages, ImageJ is relatively time-consuming, because of the need to manually select the target areas for quantification. Hence, we here evaluated the freeware programme Photopea as a potential alternative by comparing the accuracy and time required for the surface area quantification of exemplary algae compared with established ImageJ analysis. Our results show that Photopea is equally accurate as ImageJ, but 45% more time efficient. This time efficiency originates from using colour contrast that reduces the time needed to analyse each picture. Photopea thus offers an accurate, rapid, and cost-free tool to easily obtain reference data from field and laboratory experiments. This tool is particularly useful for experiments with an extensive sample size of specimens and thus can increase the power of study results.
Optimized Active Control of a Smart Cantilever Beam Using Genetic Algorithm
Authors: Awada Ali , Younes Rafic , Ilinca Adrian
Published: April 2022

Vibration is one of the most dangerous phenomena that happens to a structure. It leads to premature fatigue and eventually failure, with potentially fatal consequences. A smart structure is an excellent solution to this problem; it adds an actuator, a sensor, and an appropriate control law to the system to reduce/eliminate the vibration. This study developed a complete analytical model for a cantilever beam with a collocated PZT sensor/actuator pair. First, we used a coupling of a collocated PZT sensor and an actuator to measure and control vibration levels based on a PID control law considering the physical constraints associated with PZT operation as the voltage level of the actuator. Next, the damping coefficient of the structure was determined by using genetic algorithms best fit to satisfy specific vibration conditions. Finally, we conducted a complete optimization for sensor/actuator position and PID parameters, using genetic algorithms. Thus, this paper gives a thorough understanding of the potential vibration control of the cantilever beam.
A Web Application for Biomedical Text Mining of Scientific Literature Associated with Coronavirus-Related Syndromes: Coronavirus Finder
Authors: Armenta-Medina Dagoberto , Brambila-Tapia Aniel Jessica Leticia , Miranda-Jiménez Sabino , Rodea-Montero Edel Rafael
Published: April 2022

In this study, a web application was developed that comprises scientific literature associated with the Coronaviridae family, specifically for those viruses that are members of the Genus Betacoronavirus, responsible for emerging diseases with a great impact on human health: Middle East Respiratory Syndrome-Related Coronavirus (MERS-CoV) and Severe Acute Respiratory Syndrome-Related Coronavirus (SARS-CoV, SARS-CoV-2). The information compiled on this webserver aims to understand the basics of these viruses’ infection, and the nature of their pathogenesis, enabling the identification of molecular and cellular components that may function as potential targets on the design and development of successful treatments for the diseases associated with the Coronaviridae family. Some of the web application’s primary functions are searching for keywords within the scientific literature, natural language processing for the extraction of genes and words, the generation and visualization of gene networks associated with viral diseases derived from the analysis of latent semantic space, and cosine similarity measures. Interestingly, our gene association analysis reveals drug targets in understudies, and new targets suggested in the scientific literature to treat coronavirus.
Application of a Deep Learning System in Pterygium Grading and Further Prediction of Recurrence with Slit Lamp Photographs
Authors: Hung Kuo-Hsuan , Lin Chihung , Roan Jinsheng , Kuo Chang-Fu , Hsiao Ching-Hsi , Tan Hsin-Yuan , Chen Hung-Chi , Ma David Hui-Kang , Yeh Lung-Kun , Lee Oscar Kuang-Sheng
Published: April 2022

Background: The aim of this study was to evaluate the efficacy of a deep learning system in pterygium grading and recurrence prediction. Methods: This was a single center, retrospective study. Slit-lamp photographs, from patients with or without pterygium, were collected to develop an algorithm. Demographic data, including age, gender, laterality, grading, and pterygium area, recurrence, and surgical methods were recorded. Complex ocular surface diseases and pseudopterygium were excluded. Performance of the algorithm was evaluated by sensitivity, specificity, F1 score, accuracy, and area under the receiver operating characteristic curve. Confusion matrices and heatmaps were created to help explain the results. Results: A total of 237 eyes were enrolled, of which 176 eyes had pterygium and 61 were non-pterygium eyes. The training set and testing set were comprised of 189 and 48 photographs, respectively. In pterygium grading, sensitivity, specificity, F1 score, and accuracy were 80% to 91.67%, 91.67% to 100%, 81.82% to 94.34%, and 86.67% to 91.67%, respectively. In the prediction model, our results showed sensitivity, specificity, positive predictive value, and negative predictive values were 66.67%, 81.82%, 33.33%, and 94.74%, respectively. Conclusions: Deep learning systems can be useful in pterygium grading based on slit lamp photographs. When clinical parameters involved in the prediction of pterygium recurrence were included, the algorithm showed higher specificity and negative predictive value in prediction.