bluuebunny/crossref_metadata_2025 huggingface dataset:
Canonical and early lineage-specific stem cell types identified in planarian SirNeoblasts
Authors: Niu Kaimeng , Xu Hao , Xiong Yuanyi Zhou , Zhao Yun , Gao Chong , Seidel Chris W. , Pan Xue , Ying Yuqing , Lei Kai
Published: October 2021

Background

The pluripotent stem cells in planarians, a model for tissue and cellular regeneration, remain further identification. We recently developed a method to enrichpiwi-1+ cells inSchmidtea mediterranea, by staining cells with SiR-DNA and Cell Tracker Green, named SirNeoblasts that permits their propagation and subsequent functional study in vivo. Since traditional enrichment for planarian neoblasts by Hoechst 33342 staining generates X1 cells, blocking the cell cycle and inducing cytotoxicity, this method by SiR-DNA and Cell Tracker Green represents a complementary technological advance for functional investigation of cell fate and regeneration. However, the similarities in heterogeneity of cell subtypes between SirNeoblasts and X1 remain unknown.

Results

In this work, we performed single cell RNA sequencing of SirNeoblasts for comparison with differential expression patterns in a publicly available X1 single cell RNA sequencing data. We found first that all of the lineage-specific progenitor cells in X1 were present in comparable proportions in SirNeoblasts. In addition, SirNeoblasts contain an early muscle progenitor that is unreported in X1. Analysis of new markers for putative pluripotent stem cells identified here, with subsequent sub-clustering analysis, revealed earlier lineages of epidermal, muscular, intestinal, and pharyngeal progenitors than have been observed in X1. Using thegcmas a marker, we also identified a cell subpopulation resided in previously identifiedtgs-1+ neoblasts. Knockdown ofgcmimpaired the neoblast repopulation, suggesting a function ofgcmin neoblasts.

Conclusions

In summary, the use of SirNeoblasts will enable broad experimental advances in regeneration and cell fate specification, given the possibility for propagation and transplantation of recombinant and mutagenized pluripotent stem cells that are not previously afforded to this rapid and versatile model system.
In vivo imaging reveals mature Oligodendrocyte division in adult Zebrafish
Authors: Zou Suqi , Hu Bing
Published: October 2021

Whether mature oligodendrocytes (mOLs) participate in remyelination has been disputed for several decades. Recently, some studies have shown that mOLs participate in remyelination by producing new sheaths. However, whether mOLs can produce new oligodendrocytes by asymmetric division has not been proven. Zebrafish is a perfect model to research remyelination compared to other species. In this study, optic nerve crushing did not induce local mOLs death. After optic nerve transplantation from olig2:eGFP fish to AB/WT fish, olig2+ cells from the donor settled and rewrapped axons in the recipient. After identifying these rewrapping olig2+ cells as mOLs at 3 months posttransplantation, in vivo imaging showed that olig2+ cells proliferated. Additionally, in vivo imaging of new olig2+ cell division from mOLs was also captured within the retina. Finally, fine visual function was renewed after the remyelination program was completed. In conclusion, our in vivo imaging results showed that new olig2+ cells were born from mOLs by asymmetric division in adult zebrafish, which highlights the role of mOLs in the progression of remyelination in the mammalian CNS.
MRISCs protect colonic stem cells from inflammatory damage
Authors: Zhu Guoli , Xi Rongwen
Published: October 2021

Increasing evidence suggest functional roles of subepithelial mesenchymal niche cells in maintaining intestinal stem cells and in modulating the pathogenesis of various intestinal diseases in mammals. A recent study reported the discovery of a new population of stromal cells in mice termed MAP3K2-Regulated Intestinal Stromal Cells (MRISCs); these cells reside at the base of colonic crypt and function to protect colonic stem cells during colonic inflammation by expressing the Wnt agonist R-spondin1 (Rspo1).
Emerging liver organoid platforms and technologies
Authors: Lam Do Thuy Uyen Ha , Dan Yock Young , Chan Yun-Shen , Ng Huck-Hui
Published: October 2021

Building human organs in a dish has been a long term goal of researchers in pursue of physiologically relevant models of human disease and for replacement of worn out and diseased organs. The liver has been an organ of interest for its central role in regulating body homeostasis as well as drug metabolism. An accurate liver replica should contain the multiple cell types found in the organ and these cells should be spatially organized to resemble tissue structures. More importantly, the in vitro model should recapitulate cellular and tissue level functions. Progress in cell culture techniques and bioengineering approaches have greatly accelerated the development of advance 3-dimensional (3D) cellular models commonly referred to as liver organoids. These 3D models described range from single to multiple cell type containing cultures with diverse applications from establishing patient-specific liver cells to modeling of chronic liver diseases and regenerative therapy. Each organoid platform is advantageous for specific applications and presents its own limitations. This review aims to provide a comprehensive summary of major liver organoid platforms and technologies developed for diverse applications.
Master regulators of skeletal muscle lineage development and pluripotent stem cells differentiation
Authors: Esteves de Lima Joana , Relaix Frédéric
Published: October 2021

In vertebrates, the skeletal muscles of the body and their associated stem cells originate from muscle progenitor cells, during development. The specification of the muscles of the trunk, head and limbs, relies on the activity of distinct genetic hierarchies. The major regulators of trunk and limb muscle specification are the paired-homeobox transcription factors PAX3 and PAX7. Distinct gene regulatory networks drive the formation of the different muscles of the head. Despite the redeployment of diverse upstream regulators of muscle progenitor differentiation, the commitment towards the myogenic fate requires the expression of the early myogenic regulatory factors MYF5, MRF4, MYOD and the late differentiation marker MYOG. The expression of these genes is activated by muscle progenitors throughout development, in several waves of myogenic differentiation, constituting the embryonic, fetal and postnatal phases of muscle growth. In order to achieve myogenic cell commitment while maintaining an undifferentiated pool of muscle progenitors, several signaling pathways regulate the switch between proliferation and differentiation of myoblasts. The identification of the gene regulatory networks operating during myogenesis is crucial for the development of in vitro protocols to differentiate pluripotent stem cells into myoblasts required for regenerative medicine.
Well-posed results for nonlocal biparabolic equation with linear and nonlinear source terms
Authors: Long Le Dinh , Binh Ho Duy , Thi Kim Van Ho , Nguyen Van Thinh
Published: October 2021

In this paper, we consider the biparabolic problem under nonlocal conditions with both linear and nonlinear source terms. We derive the regularity property of the mild solution for the linear source term while we apply the Banach fixed-point theorem to study the existence and uniqueness of the mild solution for the nonlinear source term. In both cases, we show that the mild solution of our problem converges to the solution of an initial value problem as the parameter epsilon tends to zero. The novelty in our study can be considered as one of the first results on biparabolic equations with nonlocal conditions.
Efficacy of a mobile technology-based intervention for increasing parents’ safety knowledge and actions: a randomized controlled trial
Authors: McKenzie Lara B. , Roberts Kristin J. , McAdams Rebecca J. , Abdel-Rasoul Mahmoud , Kristel Orie , Szymanski Alison , Keim Sarah A. , Shields Wendy C.
Published: October 2021

Background

Leading causes of unintentional child injury such as poisoning and falls are preventable, and the majority occur in the home. Numerous home safety interventions have been developed and tested to increase safety behaviors; however, no smart phone-based applications (apps) have been developed and evaluated for this purpose. The objective of this study was to evaluate whether a mobile technology-based health behavior change intervention, the Make Safe Happen® app, was an effective tool to increase safety knowledge and safety actions/behaviors for the prevention of child unintentional injuries in and around the home.

Methods

Data were collected in pretest and posttest online surveys from an existing nationwide population-based survey panel. Intervention subjects were randomized to organically (participant-driven) use the Make Safe Happen® app for 1 week, which provided home safety information and the ability to purchase safety products, while control participants were assigned to download and use an app about a topic other than home safety. The primary outcomes of safety knowledge and home safety actions were assessed by using linear mixed model regressions with intention-to-treat analyses.

Results

A total of 5032 participants were randomized to either the intervention (n = 4182) or control (n = 850) group, with 2055 intervention participants downloading and entering their participant IDs into the Make Safe Happen® app. The online posttest survey was completed by 770 intervention and 283 control subjects. Mean knowledge parent safety score increased at a greater rate for intervention than control subjects (p < 0.0001), and at posttest was significantly higher for intervention than control subjects (p < 0.0001). The percentage of intervention subjects who reported doing all one-time and repeated safety actions significantly increased from pretest to posttest (p < 0.0001 and p = 0.0001, respectively), but there was no change among the control subjects (p = 0.1041 and p = 0.9755, respectively). At posttest, this percentage was larger for intervention than control subjects only for repeated safety actions (p = 0.0340).

Conclusions

The mobile application significantly improved safety knowledge and safety actions for participants using the Make Safe Happen® app, although loss to follow-up was a limitation. The results of this study indicate the usefulness of widespread distribution and use of the Make Safe Happen® app.

Trial registration numberNCT02751203; Registered April 26, 2016.
A randomised controlled feasibility trial of music-assisted language telehealth intervention for minimally verbal autistic children—the MAP study protocol
Authors: Williams Tim I. , Loucas Tom , Sin Jacqueline , Jeremic Mirjana , Aslett Georgia , Knight Melissa , Fincham-Majumdar Sara , Liu Fang
Published: October 2021

Background

About 30% of children with autism spectrum disorder (ASD) do not develop functional speech and remain non-verbal or minimally verbal even after years of speech, language and educational interventions. A wide range of interventions have been developed for improving communication in ASD, but none have proved effective in eliciting functional language in ASD children. Research has found that people with ASD are more likely to have perfect pitch and prefer music to language. Further, it seems that language delay tends to co-occur with better musical skills. Brain imaging research has found that music alongside words increases the attention that people with ASD pay to spoken words.

Methods

In this protocol, we describe our music-assisted programmes (MAP) that will use music to attract the attention of people with ASD to speech. MAP may open the brain pathways to language and therefore help improve communication skills for people with ASD more than standard communication protocols. In particular, we aim to develop and test whether individualised, easily used MAP would increase spoken language in 24–60-month-old, nonverbal or minimally verbal children with ASD. We will develop a structured training method, delivered through naturalistic, interactive activities (e.g. songs) to teach language to ASD children. We will test this by comparing two groups: one undertaking music-assisted programmes, and the other receiving speech and language therapy in the way that is recommended in NHS clinics. Participants will be allocated to groups randomly. The feasibility of MAP will be assessed through estimations of recruitment and retention rates, the sensitivity and reliability of the outcome measures, the intensity and frequency of the trial, the usability of the MAP app (beta version), and the burden of the assessments for the children and parents.

Discussion

This feasibility randomised controlled trial will establish the acceptability and estimate the power of the MAP intervention to improve early word learning in children with ASD. In the longer term, this research will help us develop an app for parents or carers of children with ASD to design their own songs and implement their own individualised MAP.

Trial registration

ISRCTN, ISRCTN12536062. Registered on 26 June 2019.
Retrospective study about the postoperative stability of zygomaticomaxillary complex fracture
Authors: Yang Seoghwan , Cho Jin-yong , Shim Woo-chul , Kim Sungbeom
Published: October 2021

Background

The aim of this study is to evaluate the postoperative stability of zygomaticomaxillary complex (ZMC) fractures according to the number of fixation sites and to investigate the direction of postoperative displacement of the unfixed part of the fractured segment.

Methods

This study was retrospectively performed on 38 patients who were treated by open reduction and internal fixation of ZMC fractures and were taken postoperative computed tomography (CT) between February 2012 and July 2019. The patients were classified into 3 groups: 1-point fixation, 2-point fixation, 3-point fixation according to the number of fixations. The postoperative displacement of the fractured segment was evaluated by the superimposition between postoperative CT and follow-up CT, and the postoperative stability according to the fixation sites was investigated through the amount of postoperative displacement. In addition, it was investigated in which direction the location of the fractured segment was changed in the unfixed fractured segment according to the fixation sites.

Results

The amount of postoperative displacement of the fractured segment was 0.75 ± 1.18 mm on average.

In the postoperative displacement of the distal area according to the number of fixation of the fracture, there was no statistically significant difference in the amount of displacement of the fracture (p = 0.574).

As for the direction of the change in the location of the fractured segment, 12 patients among 38 patients with the change in the location of the fractured segment were investigated, and the displacement in the medial direction (n = 11, 91.67%) was the most common in all three fixation methods.

Conclusion

In patients with a ZMC fracture who were treated by open reduction and internal fixation, the number of fixations did not make the difference in the postoperative displacement of the fracture. In addition, the fractured segment mainly changes in the medial direction after surgery, and this fact can be used as a reference for the reduction direction during surgery for the stable prognosis.
Pipeline therapies for neovascular age related macular degeneration
Authors: Arepalli Sruthi , Kaiser Peter K.
Published: October 2021

Age related macular degeneration (AMD) is the most common cause of vision loss in the elderly population. Neovascular AMD comprises 10% of all cases and can lead to devastating visual loss due to choroidal neovascularization (CNV). There are various cytokine pathways involved in the formation and leakage from CNV. Prior treatments have included focal laser therapy, verteporfin (Visudyne, Bausch and Lomb, Rochester, New York) ocular photodynamic therapy, transpupillary thermotherapy, intravitreal steroids and surgical excision of choroidal neovascular membranes. Currently, the major therapies in AMD focus on the VEGF-A pathway, of which the most common are bevacizumab (Avastin; Genentech, San Francisco, California), ranibizumab (Lucentis; Genentech, South San Francisco, California), and aflibercept (Eylea; Regeneron, Tarrytown, New York). Anti-VEGF agents have revolutionized our treatment of wet AMD; however, real world studies have shown limited visual improvement in patients over time, largely due to the large treatment burden. Cheaper alternatives, including ranibizumab biosimilars, include razumab (Intas Pharmaceuticals Ltd., Ahmedabad, India), FYB 201 (Formycon AG, Munich, Germany and Bioeq Gmbh Holzkirchen, Germany), SB-11 (Samsung Bioepsis, Incheon, South Korea), xlucane (Xbrane Biopharma, Solna, Sweden), PF582 (Pfnex, San Diego, California), CHS3551 (Coherus BioSciences, Redwood City, California). Additionally, aflibercept biosimilars under development include FYB203 (Formycon AG, Munich, Germany and Bioeq Gmbh Holzkirchen, Germany), ALT-L9 (Alteogen, Deajeon, South Korea), MYL1710 (Momenta Pharamaceuticals, Cambridge, MA, and Mylan Pharmacueticals, Canonsburg, PA), CHS-2020 (Coherus BioSciences, Redwood City, California). Those in the pipeline of VEGF targets include abicipar pegol (Abicipar; Allergan, Coolock, Dublin), OPT-302 (Opthea; OPTHEA limited; Victoria, Melbourne), conbercept (Lumitin; Chengdu Kanghong Pharmaceutical Group, Chengdu, Sichuan), and KSI-301 (Kodiak Sciences, Palo Alto, CA). There are also combination medications, which target VEGF and PDGF, VEGF and tissue factor, VEGF and Tie-2, which this paper will also discuss in depth. Furthermore, long lasting depots, such as the ranibizumab port delivery system (PDS) (Genentech, San Francisco, CA), as well as others are under evaluation. Gene therapy present possible longer treatments options as well and are reviewed here. This paper will highlight the past approved medications as well as pipeline therapies for neovascular AMD.
Integration of magnetic, gravity, and well-logging data interpretation to delineate the structural framework and formation evaluation of Bahariya Formation, North Diyur area, northern Western Desert, Egypt
Authors: Araby Walaa , Abd Samy H. , Aref Alaa E. , Al-Alfy Ibrahim , Abdullah M. M. , Elhusseiny Ahmed A.
Published: October 2021

The Bahariya Formation in Egypt's Western Desert is a major source for minerals and hydrocarbon accumulation. It is also characterized by a relatively high radiation content because it contains iron oxide deposits that attract radioactive elements. The main objectives of our study are to establish depth to basement, basement configuration and related structural elements, and thickness and configuration of the overlain sedimentary section. In addition to the analysis of well-logging data, many advanced techniques have been applied to analyze magnetic and gravity data, including depth estimation, 2D magnetic and gravity modeling, and 3D inversion of potential field data. By integrating all available data, we can determine the structural control of the study area and evaluate the subsurface parameters. Well logging has been used for interpretation of porous and permeable zones, water saturation calculation, and basic lithology identification. The depth to basement in our study ranges from −1700 to −4500 m. The basement is shallow in the northern parts of the study area and deeper in the southern parts. The main clay minerals of the formation are montmorillonite, chlorite, and a mixed clay layer. The Bahariya Formation is composed mainly of sandy clay and sandstone, and therefore it is considered an excellent reservoir.
Revisiting holistic migration
Authors: Thorkildsen Vemund S. , Gelius Leiv-J. , Robinson Enders A.
Published: October 2021

If an optical hologram is broken into pieces, a virtual object can still be reconstructed from each of the fragments. This reconstruction is possible because each diffraction point emits waves that reach every point of the hologram. Thus, the entire object is encoded into each subset of the hologram. Analogous to the broken hologram, the use of undersampled seismic data violating the Nyquist-Shannon sampling theorem may still give a well-resolved image of the subsurface. A theoretical framework of this idea has already been introduced in the literature and denoted as holistic migration. However, the general lack of seismic field data demonstrations has inspired the study presented here. Since the optical hologram is diffraction-driven, we propose to employ diffraction-separated data and not conventional reflection data as input for holistic migration. We follow the original idea and regularly undersample the data spatially. Such a sampling strategy will result in coherent noise in the image domain. We therefore introduce a novel signal processing technique to remove such noise. The feasibility of the proposed approach is demonstrated employing the Sigsbee2a controlled data set and field data from the Barents Sea.
Complex post-traumatic stress disorder in asylum seekers and victims of trafficking: treatment considerations
Authors: Jowett Sally , Argyriou Angeliki , Scherrer Odile , Karatzias Thanos , Katona Cornelius
Published: October 2021

Asylum-seekers experience high levels of traumatic events pre-, post- and during migration. Poly-traumatisation is associated with complex post-traumatic stress disorder (CPTSD), which has not yet been extensively explored in this population. CPTSD is a prevalent and highly disabling disorder in the present population requiring culturally sensitive diagnostic and treatment approaches. In this service evaluation, we evidence the high prevalence of CPTSD in an asylum-seeking sample and its association with greater distress compared with PTSD. We outline the treatment needs of asylum seekers with CPTSD.
Looking at the Future Through the Mother’s Womb: Gestational Diabetes and Offspring Fertility
Authors: Sinha Niharika , Lydia Walker Gretchen , Sen Aritro
Published: October 2021

Altered nutrition or intrauterine exposure to various adverse conditions during fetal development or earlier in a mother’s life can lead to epigenetic changes in fetal tissues, predisposing those tissues to diseases that manifest when offspring become adults. An example is a maternal obesity associated with gestational diabetes (GDM), where fetal exposure to a hyperglycemic, hyperinsulinemic, and/or hyperlipidemic gestational environment can provoke epigenetic changes that predispose offspring to various diseased conditions later in life. While it is now well established that offspring exposed to GDM have an increased risk of developing obesity, metabolic disorders, and/or cardiovascular disease in adult life, there are limited studies assessing the reproductive health of these offspring. This mini-review discusses the long-term effect of in utero exposure to GDM-associated adverse prenatal environment on the reproductive health of the offspring. Moreover, using evidence from various animal models and human epidemiological studies, this review offers molecular insight and understanding of how epigenetic reprogramming of genes culminates in reproductive dysfunction and the development of subfertility or infertility later in adult life.
Seroprevalence and characterisation of herpes simplex virus from human immunodeficiency virus in samples collected from two provinces in South Africa: a retrospective study
Authors: Obisesan Oluwafemi Samuel , Sithebe Nomathamsanqa Patricia , Mufhandu Hazel Tumelo
Published: October 2021

Background: Herpes simplex virus (HSV) is a widely distributed human pathogen that is known for its ulcerative lesions at the infection site. HSV can cause persistent infection in the host that is often followed by a period of latency within the neurons. Considering the high rate of HIV infection in South Africa, it is important to assess the seroprevalence of HSV with a focus to determine the epidemiological association between HSV-DNA and HIV-1 in the population.

Methods: A total of 44 sera samples were screened for HSV and HIV-1 using the highly sensitive enzyme-linked immunosorbent assay (ELISA). The ELISA positive samples were characterized using polymerase chain reaction (PCR) to confirm the positivity of both viruses and to further differentiate HSV into HSV-1 and -2. Thereafter, the samples were analysed for relatedness using phylogenetic analysis.

Results: Of the 44 samples, 36 (81.8%) were positive for HIV-1, while 35 (79.5%) were positive for HSV when screened with ELISA kits. The PCR results, with the use of type specific primers, showed that 4/35 (11.4%) samples were specific for HSV-1 while 30/35 (85.7%) were specific for HSV-2. Statistical analysis performed using the chi-squared goodness-of-fit test showed that there is a significant relationship between HSV-2 and HIV-1 transmission.

Conclusions: The prevalence of HSV in the population is high with an increased HSV-2 infection in women. Our study shows that some of the HSV-2 isolates are not related to the clinical isolate SD90e from South Africa, suggesting diversity in HSV-2 viral transmission.
Wprowadzenie do debaty
Authors: Gasparski Wojciech
Published: October 2021

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Decomposition-Based Approaches for a Class of Two-Stage Robust Binary Optimization Problems
Authors: Arslan Ayşe N. , Detienne Boris
Published: October 2021

In this paper, we study a class of two-stage robust binary optimization problems with objective uncertainty, where recourse decisions are restricted to be mixed-binary. For these problems, we present a deterministic equivalent formulation through the convexification of the recourse-feasible region. We then explore this formulation under the lens of a relaxation, showing that the specific relaxation we propose can be solved by using the branch-and-price algorithm. We present conditions under which this relaxation is exact and describe alternative exact solution methods when this is not the case. Despite the two-stage nature of the problem, we provide NP-completeness results based on our reformulations. Finally, we present various applications in which the methodology we propose can be applied. We compare our exact methodology to those approximate methods recently proposed in the literature under the name [Formula: see text]adaptability. Our computational results show that our methodology is able to produce better solutions in less computational time compared with the [Formula: see text]adaptability approach, as well as to solve bigger instances than those previously managed in the literature.

Summary of Contribution: Our manuscript describes an exact solution approach for a class of robust binary optimization problems with mixed-binary recourse and objective uncertainty. Its development reposes first on a reformulation of the problem, then a carefully constructed relaxation of this reformulation. Our solution approach is designed to exploit the two-stage and binary structure of the problem for effective resolution. In its execution, it relies on the branch-and-price algorithm and its efficient implementation. With our computational experiments, we show that our proposed exact solution method outperforms the existing approximate methodologies and, therefore, pushes the computational envelope for the class of problems considered.
Mobile Consumer Scanning Technology: A Replacement for Interorganizational Information Systems for Demand Information Learning in Supply Chains?
Authors: Shi Ye , Alwan Layth C. , Raghunathan Srinivasan , Yu Yugang , Yue Xiaohang
Published: October 2021

Recently, firms in supply chains have begun to deploy popular mobile apps (e.g., WeChat) into their supply chain practices to improve demand visibility. These efforts rely on consumers to scan the products they purchase using these apps, which we refer to as consumer scanning technology (CST). CST can be an alternative to the conventional interorganizational information technology (IOIT) that relies on collaboration between supply chain firms. This paper develops a theoretical model to examine the value of CST to learn supply chain (demand) information and the impact of CST on IOIT. Using an extensive simulation analysis based on real-world data from a manufacturer that has implemented a CST program, we show that the value of CST to a manufacturer can be substantial and provide insights into how market conditions affect the value.
Microcurrent stimulates cell proliferation and modulates cytokine release in fibroblast cells
Authors: Bravo Mariana Prado , Soares Glaciane Pozza , Daniele de Oliveira Priscila , Szezerbaty Stheacy Kelly , Frederico Regina Celia Poli , Maia Luciana Prado
Published: October 2021

Aims:

To analyse the effects of microcurrent on L929 fibroblast cell culture.

Methods:

Cells were cultivated in six-well plates at densities of 5×104, 1×105, 3×105and 5×105cells/well to determine the best plating density. Subsequently, two methods of current application were tested: with a paper cone coupled to the electrode (M1) and with the electrode directly inside the well (M2). Then, streams of 60µA (G60), 100µA (G100), 500µA (G500) and 900µA (G900) were applied to the cells (n=3) once a day for three minutes, for a period of one (T1), two (T2) and three days (T3). The MTT assay method was used to evaluate cell proliferation. For the quantification of the inflammatory markers by flow cytometry, the group and time that presented the best results were selected.

Results:

The ideal plating density was established as 1x105cells/well and M2 as the best application method. An increase in cell viability was observed at all intensities from T1 to T2, but with no significant differences. From T2 to T3, there was a decrease in viability in all groups, with a significant difference only in G500 (p<0.05). Flow cytometry was performed in the GC and G900 groups at T2. It was possible to observe an increase of 0.56pg/ml in Interleukin (IL)-17 and a decrease of 5.45pg/ml in IL-2.

Conclusion:

This study showed that two applications of microcurrent increases cell proliferation and modulates the inflammatory response, aiding tissue regeneration and playing a key role in rehabilitation.
Stem cells and repair of necrosis after dermolipectomy: a case study
Authors: Hernández-Patiño Rafael Ivan , Rossani German , Arriola Augusto , Tello-Majluf Daniela , Vargas Jhony De la Cruz
Published: October 2021

Skin ulcers secondary to necrosis post-dermolipectomy occur infrequently. Despite their rarity and because of their complex pathopathophysiological nature, their resolution is a major challenge for plastic surgeons. This study is to recognise the safety and efficiency of use of CD34 stem cells (SC), obtained from the patient's bone marrow, within a solid autologous fibrin scaffold in the case of female patient with an ulcer with torpid evolution as a result of an abdominal dermolipectomy. The concept was to regenerate the affected tissue, and to induce and promote the formation of granulation tissue that is compact enough to stimulate repair by secondary intention or facilitating surgical closure. We applied a technique under specific protocols to obtain the stem cells. The healing technique—the application of CD34 stem cells without any surgical intervention—was applied at two ambulatory sessions. There were no complications or concomitant infections, and recovery was complete within five weeks via secondary intention healing, achieving a significant and satisfactory healing response in the patient, improving both her physical and mental health. In this case study, CD34 stem cells were used successfully in the treatment of a hard-to-heal ulcer. It is necessary to carry out further investigations with a greater number of patients to substantiate the use of this intervention in patients with hard-to-heal wounds.
Anterior versus posterior fusion surgery in idiopathic scoliosis: A comparison of health-related quality of life and radiographic outcomes in Lenke 5C curves - results from the Swedish spine registry
Authors: Charalampidis Anastasios , Möller Hans , Gerdhem Paul
Published: October 2021

Purpose

To compare health-related quality of life and radiographic outcomes in patients treated with either anterior or posterior fusion surgery for Lenke 5C type idiopathic scoliosis.

Methods

We used data from the Swedish spine registry and identified 59 patients with idiopathic scoliosis treated with fusion for Lenke 5C type curves; 27 patients underwent anterior surgery and 32 underwent posterior surgery. All patients had pre- and postoperative radiographic data and postoperative clinical data at a minimum of two years after surgery. Patient-reported outcomes measures included the Scoliosis Research Society (SRS)-22r, EuroQoL 5 dimensions 3 levels (EQ-5D-3L), EQ-visual analogue scale (VAS) and VAS for back pain. Radiographic assessment included measurement of the angle of the major curve, disc angulation below the lowest instrumented vertebra, curve flexibility, rate of curve correction, differences in sagittal parameters, number of fused vertebrae and length of fusion.

Results

The mean age at surgery was 16 years in both groups. The mean follow-up time was 3.8 years. There were no significant differences in the SRS-22r score and EQ-5D-3L index at follow-up (all p ≥ 0.2). Postoperatively, both the anterior and posterior fusion group demonstrated a significant correction of the major curve (p ≤ 0.001) with no significant difference of the correction rate between the groups (p = 0.4). The posterior fusion group had shorter operative time (p < 0.001) and higher perioperative blood loss (p = 0.004) while the anterior group had lower number of fused vertebrae (p< 0.001).

Conclusion

The type of surgical approach for Lenke 5C curves is not associated with differences in health-related quality of life, despite the lower number of fused vertebrae after anterior surgery.

Level of Evidence

III
Hybrid-net: a two-to-one deep learning framework for three-wavelength phase-shifting interferometry
Authors: Li Jiaosheng , Zhang Qinnan , Zhong Liyun , Lu Xiaoxu
Published: October 2021

In this paper, we propose a two-to-one deep learning (DL) framework for three- wavelength phase-shifting interferometry. The interferograms at two different wavelengths are used as the input of the proposed hybrid-net, and the interferogram of the third wavelength is used as the output. Using the advantages of the hybrid learning network, the interferogram of the third wavelength can be obtained accurately. Finally, the three-wavelength phase-shifting interferometry is realized. Compared with the previous DL-based dual-wavelength interferometry (DWI), the proposed method can further improve the measurement range of the sample without changing the DWI system. Especially for the independent step sample, the problem of limited measurement range is solved due to the input of auxiliary information. More importantly, the third wavelength can be set freely according to the measurement requirements, which is no longer limited by the actual laser and can provide more measuring ruler for phase measurement. Both experimental results and simulation analysis demonstrate the proposed method in the feasibility and the performance in improving the measurement range.
Autosetting soliton pulsation in a fiber laser by an improved depth-first search algorithm
Authors: Zheng Pei-Zhu , Li Ti-Jian , Xia Han-Ding , Feng Meng-Jun , Liu Meng , Ye Bo-Lin , Luo Ai-Ping , Xu Wen-Cheng , Luo Zhi-Chao
Published: October 2021

Soliton pulsation is one of the most fascinating phenomena in ultrafast fiber lasers, owing to its rich nonlinear dynamics and potential generation of high peak power pulse. However, it is still a challenge to efficiently search for pulsating soliton in fiber lasers because it requires a fine setting of laser cavity parameters. Here, we report the autosetting soliton pulsation in a passively mode-locked fiber laser. The parameters of electronic polarization controller are intelligently adjusted to search for pulsating soliton state by the improved depth-first search algorithm. Moreover, the intensity modulation depth of pulsating soliton could be flexibly controlled. These findings indicate that the intelligent control of a fiber laser is an effective way to explore on-demand soliton dynamics and is also beneficial to the optimization of ultrafast laser performance.
Broadband surface wave coupler with low infrared emission and microwave reflection
Authors: Liu Tonghao , Meng Yueyu , Ma Hua , Zhu Ruichao , Huang Sining , Xu Cuilian , Zhang Lei , Wang Jiafu , Qu Shaobo
Published: October 2021

Metasurfaces possess excellent capabilities to flexibly manipulate electromagnetic waves in multiple frequency domains, which show great potential application in multispectral stealth. Herein, a broadband surface waves coupler based on the design of thin Pancharatnam–Berry (PB) phase gradient metasurfaces (PGMs) of thickness 0.12λ0 is proposed to reduce infrared emission and microwave reflection simultaneously. Low infrared emission results from the high filling ratio of the indium-tin-oxide (ITO) on the surface, and low microwave reflection results from the conversion from propagating waves to surface waves. Intriguingly, this design is also capable of acting as a simple circular polarized (CP) discriminator because orthogonal CP waves are coupled into surface waves propagating along opposite directions. A proof-of-concept prototype is simulated and measured to validate the effectiveness of our methodology. The results indicate that the broadband surface waves coupler shows low infrared emissivity less than 0.28 from 3 to 14 µm and has microwave reflection reduction larger than 10 dB in 7.3-9.5 GHz. The exceptional performances of the proposed broadband surface waves coupler make us believe that our design offers an alternative strategy for multispectral stealth and multifunctional application.
Enhanced solar water splitting using plasmon-induced resonance energy transfer and unidirectional charge carrier transport
Authors: Jia Huaping , Wong Yat Lam , Wang Bingzhe , Xing Guichuan , Tsoi Chi Chung , Wang Meiling , Zhang Wendong , Jian Aoqun , Sang Shengbo , Lei Dangyuan , Zhang Xuming
Published: October 2021

Solar water splitting by photoelectrochemical (PEC) reactions is promising for hydrogen production. The gold nanoparticles (AuNPs) are often applied to promote the visible response of wideband photocatalysts. However, in a typical TiO2/AuNPs structure, the opposite transfer direction of excited electrons between AuNPs and TiO2 under visible light and UV light severely limits the solar PEC performance. Here we present a unique Pt/TiO2/Cu2O/NiO/AuNPs photocathode, in which the NiO hole transport layer (HTL) is inserted between AuNPs and Cu2O to achieve unidirectional transport of charge carriers and prominent plasmon-induced resonance energy transfer (PIRET) between AuNPs and Cu2O. The measured applied bias photon-to-current efficiency and the hydrogen production rate under AM 1.5G illumination can reach 1.5% and 16.4 μmol·cm-2·h-1, respectively. This work is original in using the NiO film as the PIRET spacer and provides a promising photoelectrode for energy-efficient solar water splitting.
Selection and cloning of periodic optical patterns with a cold Rydberg atomic gas
Authors: Shi Zeyun , Huang Guoxiang
Published: October 2021

We show that periodic optical patterns formed in a cold Rydberg atomic gas via electromagnetically induced transparency (EIT) can be selected by using a weakly modulated control laser field. We also show that the (hexagonal, stripe, square, etc.) patterns prepared in one probe laser field can be cloned onto another one with high fidelity via a double EIT.
Hyperuniform scalar random fields for lensless, multispectral imaging systems
Authors: Chen Yuyao , Britton Wesley A. , Dal Negro Luca
Published: October 2021

We propose a novel framework for the systematic design of lensless imaging systems based on the hyperuniform random field solutions of nonlinear reaction-diffusion equations from pattern formation theory. Specifically, we introduce a new class of imaging point-spread functions (PSFs) with enhanced isotropic behavior and controllable sparsity. We investigate PSFs and modulated transfer functions for a number of nonlinear models and demonstrate that two-phase isotropic random fields with hyperuniform disorder are ideally suited to construct imaging PSFs with improved performances compared to PSFs based on Perlin noise. Additionally, we introduce a phase retrieval algorithm based on non-paraxial Rayleigh–Sommerfeld diffraction theory and introduce diffractive phase plates with PSFs designed from hyperuniform random fields, called hyperuniform phase plates (HPPs). Finally, using high-fidelity object reconstruction, we demonstrate improved image quality using engineered HPPs across the visible range. The proposed framework is suitable for high-performance lensless imaging systems for on-chip microscopy and spectroscopy applications.
Efficient nonlinear compression of a thin-disk oscillator to 8.5  fs at 55  W average power
Authors: Barbiero Gaia , Wang Haochuan , Graßl Martin , Gröbmeyer Sebastian , Kimbaras Džiugas , Neuhaus Marcel , Pervak Vladimir , Nubbemeyer Thomas , Fattahi Hanieh , Kling Matthias F.
Published: October 2021

We demonstrate an efficient hybrid-scheme for nonlinear pulse compression of high-power thin-disk oscillator pulses to the sub-10 fs regime. The output of a home-built, 16 MHz, 84 W, 220 fs Yb:YAG thin-disk oscillator at 1030 nm is first compressed to 17 fs in two nonlinear multipass cells. In a third stage, based on multiple thin sapphire plates, further compression to 8.5 fs with 55 W output power and an overall optical efficiency of 65% is achieved. Ultrabroadband mid-infrared pulses covering the spectral range 2.4-

8

µ
m

were generated from these compressed pulses by intra-pulse difference frequency generation.
Short-term elevations in glucocorticoids do not alter telomere lengths: A systematic review and meta-analysis of non-primate vertebrate studies
Authors: Zane Lauren , Ensminger David C. , Vázquez-Medina José Pablo
Published: October 2021

Background

The neuroendocrine stress response allows vertebrates to cope with stressors via the activation of the Hypothalamic-Pituitary-Adrenal (HPA) axis, which ultimately results in the secretion of glucocorticoids (GCs). Glucocorticoids have pleiotropic effects on behavior and physiology, and might influence telomere length dynamics. During a stress event, GCs mobilize energy towards survival mechanisms rather than to telomere maintenance. Additionally, reactive oxygen species produced in response to increased GC levels can damage telomeres, also leading to telomere shortening. In our systematic review and meta-analysis, we tested whether GC levels impact telomere length and if this relationship differs among time frame, life history stage, or stressor type. We hypothesized that elevated GC levels are linked to a decrease in telomere length.

Methods

We conducted a literature search for studies investigating the relationship between telomere length and GCs in non-human vertebrates using four search engines: Web of Science, Google Scholar, Pubmed and Scopus, last searched on September 27th, 2020. This review identified 31 studies examining the relationship between GCs and telomere length. We pooled the data using Fisher’s Z for 15 of these studies. All quantitative studies underwent a risk of bias assessment. This systematic review study was registered in the Open Science Framework Registry (https://osf.io/rqve6).

Results

The pooled effect size from fifteen studies and 1066 study organisms shows no relationship between GCs and telomere length (Fisher’s Z = 0.1042, 95% CI = 0.0235; 0.1836). Our meta-analysis synthesizes results from 15 different taxa from the mammalian, avian, amphibian groups. While these results support some previous findings, other studies have found a direct relationship between GCs and telomere dynamics, suggesting underlying mechanisms or concepts that were not taken into account in our analysis. The risk of bias assessment revealed an overall low risk of bias with occasional instances of bias from missing outcome data or bias in the reported result.

Conclusion

We highlight the need for more targeted experiments to understand how conditions, such as experimental timeframes, stressor(s), and stressor magnitudes can drive a relationship between the neuroendocrine stress response and telomere length.
Sequencing DNA with nanopores: Troubles and biases
Authors: Delahaye Clara , Nicolas Jacques
Published: October 2021

Oxford Nanopore Technologies’ (ONT) long read sequencers offer access to longer DNA fragments than previous sequencer generations, at the cost of a higher error rate. While many papers have studied read correction methods, few have addressed the detailed characterization of observed errors, a task complicated by frequent changes in chemistry and software in ONT technology. The MinION sequencer is now more stable and this paper proposes an up-to-date view of its error landscape, using the most mature flowcell and basecaller. We studied Nanopore sequencing error biases on both bacterial and human DNA reads. We found that, although Nanopore sequencing is expected not to suffer from GC bias, it is a crucial parameter with respect to errors. In particular, low-GC reads have fewer errors than high-GC reads (about 6% and 8% respectively). The error profile for homopolymeric regions or regions with short repeats, the source of about half of all sequencing errors, also depends on the GC rate and mainly shows deletions, although there are some reads with long insertions. Another interesting finding is that the quality measure, although over-estimated, offers valuable information to predict the error rate as well as the abundance of reads. We supplemented this study with an analysis of a rapeseed RNA read set and shown a higher level of errors with a higher level of deletion in these data. Finally, we have implemented an open source pipeline for long-term monitoring of the error profile, which enables users to easily compute various analysis presented in this work, including for future developments of the sequencing device. Overall, we hope this work will provide a basis for the design of better error-correction methods.