bluuebunny/crossref_metadata_2025 huggingface dataset:
The implications of warmer winters for ice climbing: A case study of the Mount Washington Valley, New Hampshire, USA
Authors: Voorhis Jimmy , McDowell Graham , Burakowski Elizabeth , Luneau Taylor
Published: March 2023

Ice climbing is important to the culture and economies of mountain communities worldwide. However, warming winters call into question the future of livelihoods associated with ice climbing. In response, this case study presents observed and simulated ice climbing conditions in the Mount Washington Valley, New Hampshire, USA, as well as local climbing guide's experiences of and responses to these changes. First, variability in ice conditions were evaluated by classifying and summarizing ice characteristics depicted in a 20-year collection of conditions reports (n = 372) including photos and written observations for a benchmark ice climb (Standard Route). Next, climate model ensembles were used to simulate probable changes in future ice season lengths according to intermediate and high climate forcing scenarios (i.e., RCP 4.5 & RCP 8.5). Finally, a survey and focus group were conducted with Mount Washington Valley ice climbing guides to examine observations and lived experiences of warming winters. This study, which is the first formal assessment of the implications of warming winters for ice climbing, reveals significant effects of climate change for current and projected ice climbing conditions as well as marked, and often differentiated, vulnerability and adaptability to these changes amongst climbing guides. The unique mixed-methods approach used is applicable in other locales where climate change is impacting ice climbing activities and associated livelihoods.
Comparison of humoral and cellular immune responses between ChAd-BNT heterologous vaccination and BNT-BNT homologous vaccination following the third BNT dose: A prospective cohort study
Authors: Sim Wooho , Kang Hyunhye , Jung Jin , Lee Jihyun , Ko Geon Young , Park Hye-Sun , Choi Jeewan , Park Kinam , Oh Eun-Jee
Published: March 2023

Introduction

The differential immune responses after two additional BNT162b2 (BNT) booster doses between ChAdOx1 nCoV-10 (ChAd)-primed and BNT-primed groups have not been elucidated. The aim of this study was to compare vaccine-induced humoral and cellular immune responses and evaluate breakthrough infection between the two vaccination strategies.

Methods

In 221 healthy subjects (111 in the ChAd group), longitudinal immune responses were monitored at 3, 4, and 6 months after the 2nd dose and 1, 3, and 6 months after the 3rd dose. Humoral immunity was measured by two fully automated chemiluminescent immunoassays (Elecsys and Abbott) and a surrogate virus neutralization test (sVNT). Cellular immunity was assessed by two interferon-γ (IFN-γ) release assays (QuantiFERON SARS-CoV-2 and Covi-FERON).

Results

After the 2nd dose of BNT vaccination, total antibody levels were higher in the ChAd group, but IgG antibody and sVNT results were higher in the BNT group. Following the 3rd dose vaccination, binding antibody titers were significantly elevated in both groups (ChAD-BNT; 15.4 to 17.8-fold, BNT-BNT; 22.2 to 24.6-fold), and the neutralizing capacity was increased by 1.3-fold in both cohorts. The ChAd-BNT group had lower omicron neutralization positivity than the BNT-BNT group (P = 0.001) at 6 months after the 3rd dose. Cellular responses to the spike antigen also showed 1.7 to 3.0-fold increases after the 3rd dose, which gradually declined to the levels equivalent to before the 3rd vaccination. The ChAd cohort tended to have higher IFN-γ level than the BNT cohort for 3-6 months after the 2nd and 3rd doses. The frequency of breakthrough infection was higher in the ChAd group (44.8%) than in the BNT group (28.1%) (P = 0.0219). Breakthrough infection induced increased humoral responses in both groups, and increase of cellular response was significant in the ChAd group.

Discussion

Our study showed differential humoral and cellular immune responses between ChAd-BNT-BNT heterologous and BNT-BNT-BNT homologous vaccination cohorts. The occurrence of low antibody levels in the ChAd-primed cohort in the humoral immune response may be associated with an increased incidence of breakthrough infections. Further studies are needed on the benefits of enhanced cellular immunity in ChAd-primed cohorts.
A novel liver zonation phenotype-associated molecular classification of hepatocellular carcinoma
Authors: Zhang Tao , Gu Jian , Wang Xinyi , Lu Yaoyao , Cai Kailin , Li Huili , Nie Yingli , Chen Xiangdong , Wang Jiliang
Published: March 2023

Background

Liver zonation is a unique phenomenon in which the liver exhibits distinct functions among hepatocytes along the radial axis of the lobule. This phenomenon can cause the sectionalized initiation of several liver diseases, including hepatocellular carcinoma (HCC). However, few studies have explored the zonation features of HCC.

Methods

Four single-cell RNA sequencing datasets were used to identify hepatocyte-specific zonation markers. Integrative analysis was then performed with a training RNA-seq cohort (616 HCC samples) and an external validating microarray cohort (285 HCC samples) from the International Cancer Genome Consortium, The Cancer Genome Atlas, Gene Expression Omnibus, and EMBL’s European Bioinformatics Institute for clustering using non-negative matrix factorization consensus clustering based on zonation genes. Afterward, we evaluated the prognostic value, clinical characteristics, transcriptome and mutation features, immune infiltration, and immunotherapy response of the HCC subclasses.

Results

A total of 94 human hepatocyte-specific zonation markers (39 central markers and 55 portal markers) were identified for the first time. Subsequently, three subgroups of HCC, namely Cluster1, Cluster2, and Cluster3 were identified. Cluster1 exhibited a non-zonational-like signature with the worst prognosis. Cluster2 was intensively associated with a central-like signature and exhibited low immune infiltration and sensitivity toward immune blockade therapy. Cluster3 was intensively correlated with a portal-like signature with the best prognosis. Finally, we identified candidate therapeutic targets and agents for Cluster1 HCC samples.

Conclusion

The current study established a novel HCC classification based on liver zonation signature. By classifying HCC into three clusters with non-zonational-like (Cluster1), central-like (Cluster2), and portal-like (Cluster3) features, this study provided new perspectives on the heterogeneity of HCC and shed new light on delivering precision medicine for HCC patients.
A phenomic modeling approach for using chlorophyll-a fluorescence-based measurements on coral photosymbionts
Authors: Hoadley Kenneth D. , Lockridge Grant , McQuagge Audrey , Pahl K. Blue , Lowry Sean , Wong Sophie , Craig Zachary , Petrik Chelsea , Klepac Courtney , Muller Erinn M.
Published: March 2023

We test a newly developed instrument prototype which utilizes time-resolved chlorophyll-a fluorescence techniques and fluctuating light to characterize Symbiodiniaceae functional traits across seven different coral species under cultivation as part of ongoing restoration efforts in the Florida Keys. While traditional chlorophyll-a fluorescence techniques only provide a handful of algal biometrics, the system and protocol we have developed generates > 1000 dynamic measurements in a short (~11 min) time frame. Resulting ‘high-content’ algal biometric data revealed distinct phenotypes, which broadly corresponded to genus-level Symbiodiniaceae designations determined using quantitative PCR. Next, algal biometric data from Acropora cervicornis (10 genotypes) and A. palmata (5 genotypes) coral fragments was correlated with bleaching response metrics collected after a two month-long exposure to high temperature. A network analysis identified 1973 correlations (Spearman R > 0.5) between algal biometrics and various bleaching response metrics. These identified biomarkers of thermal stress were then utilized to train a predictive model, and when tested against the same A. cervicornis and A. palmata coral fragments, yielded high correlation (R = 0.92) with measured thermal response (reductions in absorbance by chlorophyll-a). When applied to all seven coral species, the model ranked fragments dominated by Cladocopium or Breviolum symbionts as more bleaching susceptible than corals harboring thermally tolerant symbionts (Durusdinium). While direct testing of bleaching predictions on novel genotypes is still needed, our device and modeling pipeline may help broaden the scalability of existing approaches for determining thermal tolerance in reef corals. Our instrument prototype and analytical pipeline aligns with recent coral restoration assessments that call for the development of novel tools for improving scalability of coral restoration programs.
Behavior of geomaterial composite using sugar cane bagasse ash under compressive and flexural loading
Authors: Nikhade Harshal , Birali Ram Rathan Lal , Ansari Khalid , Khan Mohammad Arsalan , Najm Hadee Mohammed , Anas S. M. , Mursaleen Mohammad , Hasan Mohd Abul , Islam Saiful
Published: March 2023

The sugar industry produces a huge quantity of sugar cane bagasse ash in India. Dumping massive quantities of waste in a non-eco-friendly manner is a key concern for developing nations. The main focus of this study is the development of a sustainable geomaterial composite with higher strength capabilities (compressive and flexural). To develop this composite, sugarcane bagasse ash (SA), glass fiber (GF), and blast furnace slag (BF) are used. Ash generated from burning sugar cane in the sugar industry is known as sugar cane bagasse. To check the suitability of this secondary waste for use in civil engineering and to minimize risk to the environment in the development of sustainable growth, a sequence of compressive and flexural strength tests was performed on materials prepared using sugar cane bagasse ash (SA) reinforced by glass fiber (GF) in combination with blast furnace slag (BF) and cement (CEM). The effects of the mix ratios of glass fiber to bagasse ash (0.2%–1.2%), blast furnace slag to the weight of bagasse ash (10%), cement binding to bagasse ash (10%–20%), and water to sugar cane bagasse ash (55%) regarding the flexural strength, compressive strength, density, tangent modulus, stress–strain pattern, and load–deflection curve of the prepared materials were studied. According to the findings, compressive strength achieved a maximum strength of 1055.5 kPa and ranged from 120 to 1055.5 kPa, and the flexural strength achieved a maximum strength of 217 kPa and ranged from 80.1 to 217 kPa at different mix ratio percentages. The value of the initial tangent modulus for the cube specimens ranged between 96 and 636 MPa. For compression specimens with 20% cement, the density decreased from 1320.1 to 1265 kg/m3, and the flexural strength decreased from 1318 to 1259.6 kg/m3. With limitation in lower percentages of C/SA, the specimen cannot sustain its shape even after curing period. In comparing the previous research with the present experimental work, it was observed that the material proposed here is lightweight and can be utilised as a filler substance in weak compressible soils to improve their load-bearing capacity.
Tunable, reconfigurable, and programmable acoustic metasurfaces: A review
Authors: Zabihi Ali , Ellouzi Chadi , Shen Chen
Published: March 2023

The advent of acoustic metasurfaces (AMs), which are the two-dimensional equivalents of metamaterials, has opened up new possibilities in wave manipulation using acoustically thin structures. Through the interaction between the acoustic waves and the subwavelength scattering, AMs exhibit versatile capabilities to control acoustic wave propagation such as by steering, focusing, and absorption. In recent years, this vibrant field has expanded to include tunable, reconfigurable, and programmable control to further expand the capacity of AMs. This paper reviews recent developments in AMs and summarizes the fundamental approaches for achieving tunable control, namely, by mechanical tuning, active control, and the use of field-responsive materials. An overview of basic concepts in each category is first presented, followed by a discussion of their applications and details about their performance. The review concludes with the outlook for future directions in this exciting field.
A force controlled tribometer for pre-sliding measurements at the nanometer scale
Authors: Du Junxiao , Franklin Steve , Weber Bart
Published: March 2023

In the pre-sliding friction regime, interfaces partially stick and partially slip. The pre-slip is thought to be locally initiated at regions of the interface where the ratio of shear stress to normal stress exceeds a critical value. The displacements involved in pre-slip can be limited to the nanoscale, especially for stiff interfaces. Furthermore, little is known experimentally about the interplay between surface topography, pre-sliding behavior and wear. In this work, we introduce a pre-sliding tribometer that enables the study of how the pre-sliding friction at various types of ball-on-flat interfaces evolves as a function of wear. Polytetrafluoroethylene-on-silicon (PTFE-on-Si) pre-sliding measurements covering interfacial displacements up to 50 nm, conducted with the new instrument, show good agreement with Mindlin theory predictions, without adjustable parameters.
Incidence and clinical outcomes of bacterial superinfections in critically ill patients with COVID-19
Authors: Yoon Si Mong , Lee Jinwoo , Lee Sang-Min , Lee Hong Yeul
Published: March 2023

Background

Bacterial superinfection is not uncommon in critically ill patients with coronavirus disease (COVID-19) pneumonia requiring intensive care unit (ICU) treatment. However, there is still a lack of evidence related to bacterial superinfection and their clinical significance in critically ill patients with COVID-19. Therefore, we assessed the incidence of bacterial superinfections and their effects on clinical outcomes in critically ill patients with COVID-19.

Materials and methods

This single-center retrospective cohort study analyzed critically ill patients with COVID-19 admitted to the ICU at a tertiary academic hospital between February 2020 and December 2021. We reviewed data including patient demographics, clinical and microbiological characteristics, and outcomes.

Results

During the study period, 106 patients (median [IQR] age, 67 [58–75] years) were included, of which 32 (30%) were diagnosed with bacterial superinfections. Of these, 12 cases (38%) were associated with multidrug-resistant pathogens. Klebsiella aerogenes (6 cases [19%]) and Klebsiella pneumoniae (6 cases [19%]) were the most common pathogens associated with superinfections. The median time to bacterial superinfection was 13 (IQR, 9–20) days after ICU admission. Patients with bacterial superinfections had significantly fewer ventilator-free days on day 28 (0 [IQR, 0–0] days) than those without bacterial superinfections (19 [IQR, 0–22] days) (p < 0.001). Patients with bacterial superinfections had a longer ICU length of stay (32 [IQR, 9–53] days) than those without bacterial superinfections (11 [IQR, 7–18] days) (p < 0.001). Additionally, they had a longer hospital length of stay after ICU admission (39 [IQR, 18–62] days) than those without bacterial superinfections (18 [IQR, 12–37] days) (p = 0.001). There were no differences in ICU mortality or in-hospital mortality between the two groups. In the multivariable analysis, higher SAPS II score (OR, 2.697; 95% CI, 1.086–6.695) and thrombocytopenia (OR, 3.318; 95% CI, 1.355–8.123) were identified as risk factors for development of bacterial superinfection.

Conclusion

In critically ill patients with COVID-19, bacterial superinfections were common, and more than one-third of the bacterial superinfection cases were caused by multidrug-resistant pathogens. As patients with bacterial superinfections had worse clinical outcomes, the development of bacterial superinfections should be actively monitored.
Mortality and survival in nonagenarians during the COVID-19 pandemic: Unstable equilibrium of aging
Authors: Kashtanova Daria A. , Erema Veronika V. , Gusakova Maria S. , Sutulova Ekaterina R. , Yakovchik Anna Yu. , Ivanov Mikhail V. , Taraskina Anastasiia N. , Terekhov Mikhail V. , Matkava Lorena R. , Rumyantseva Antonina M. , Yudin Vladimir S. , Akopyan Anna A. , Strazhesko Irina D. , Kordiukova Irina S. , Akinshina Alexandra I. , Makarov Valentin V. , Tkacheva Olga N. , Kraevoy Sergey A. , Yudin Sergey M.
Published: March 2023

Introduction

Aging puts the human body under an immense stress and makes it extremely susceptible to many diseases, often leading to poor outcomes and even death. Long-living individuals represent a unique group of people who withstood the stress of time and offer an abundance of information on the body’s ability to endure the pressure of aging. In this study, we sought to identify predictors of overall one-year mortality in 1641 long-living individuals. Additionally, we analyzed risk factors for COVID-19-related morality, since statistics demonstrated an extreme vulnerability of older adults.

Methods

We conducted a two-stage evaluation, including a comprehensive geriatric assessment for major aging-associated: frailty, cognitive impairment, frontal lobe dysfunction, chronic pain, anxiety, risk of falls, sensory deficit, depression, sarcopenia, risk of malnutrition, fecal and urinary incontinence, dependence in Activities of Daily Living, dependence in Instrumental Activities of Daily Living, polypragmasia, and orthostatic hypotension; extensive blood testing, a survey, and a one-year follow-up interview.

Results

The most reliable predictors of overall mortality were cognitive impairment, malnutrition, frailty, aging-associated diseases and blood markers indicating malnutrition-induced metabolic dysfunctions (decreased levels of protein fractions, iron, 25-hydroxyvitamin D, and HDL), and aging biomarkers, such as IGF-1 and N-terminal pro b-type natriuretic peptide. In post-COVID 19 participants, the most significant mortality predictors among geriatric syndromes were depression, frontal lobe dysfunction and frailty, and similar to overall mortality blood biomarkers - 25-hydroxyvitamin D, IGF-1, HDL as well as high white blood cell, neutrophils counts and proinflammatory markers. Based on the results, we built a predictive model of overall mortality in the long-living individuals with f-score=0.76.

Conclusion

The most sensitive and reliable predictors of mortality were modifiable. This is another evidence of the critical importance of proper geriatric care and support for individuals in their “golden years”. These results could facilitate geriatric institutions in their pursuit for providing improved care and could aid physicians in detecting early signs of potentially deadly outcomes. Additionally, our findings could be used in developing day-to-day care guidelines, which would greatly improve prevention statistics.
A bibliometric analysis of systematic reviews and meta-analyses in ophthalmology
Authors: Fu Yihang , Mao Yuxiang , Jiang Shuangyan , Luo Sheng , Chen Xiaoyun , Xiao Wei
Published: March 2023

Background

Bibliometric analysis is a quantitative method which applies mathematical and statistical tools to evaluate the inter-relationships and impacts of publications, authors, institutions and countries in a specific research area. Systematic reviews and meta-analyses (SRMAs) are summaries of the best available evidence to address a specific research question via comprehensively literature search, in-depth analysis and synthesis of results. To date, there have been several studies summarizing the publication trends of SRMAs in research specialties, however, none has conducted specifically in ophthalmology. The purpose of this study is to establish the scientometric landscape of SRMAs published in the field of ophthalmology over time.

Methods

We retrieved relevant ophthalmological SRMAs and the corresponding bibliometric parameters during 2000 to 2020 from Web of Science Core Collection. Bibliometric analysis was performed using bibliometrix package. Pre-registration and guideline compliance of each article was independently assessed by two investigators.

Results

A total of 2,660 SRMAs were included, and the average annual growth rate was 21.26%. China and the United States were the most productive countries, while Singapore was the country with the highest average citations per document. Wong TY was not only the most productive, but also the most frequently cited author. The most productive affiliation was National University of Singapore (n = 236). Systematic reviews and meta-analyses output in most subspecialties had steadily increased with retina/vitreous (n = 986), glaucoma (n = 411) and cornea/external diseases (n = 303) constantly as the most dominant fields. Rates of pre-registration and guideline compliance had dramatically increased over time, with 20.0 and 63.5% of article being pre-registered and reported guideline in 2020, respectively. However, SRMAs published on ophthalmology journals tended to be less frequently pre-registered and guideline complied than those on non-ophthalmology journals (both p < 0.001).

Conclusion

The annual output of SRMAs has been rapidly increasing over the past two decades. China and the United States were the most productive countries, whereas Singapore has the most prolific and influential scholar and institution. Raising awareness and implementation of SRMAs pre-registration and guideline compliance is still necessary to ensure quality, especially for ophthalmology journals.
Challenges of cardiac sarcoidosis
Authors: Strambu Irina R.
Published: March 2023

Sarcoidosis is a multisystem granulomatosis of unknown origin, which can involve almost any organ. Most frequently the disease involves the lungs and mediastinal lymph nodes, but it can affect the skin, the eyes, nervous system, the heart, kidneys, joints, muscles, calcium metabolism, and probably any other anecdotical organ involvement. Cardiac sarcoidosis is one of the most challenging involvements, as it can lead to cardiac mortality and morbidity, and also because the diagnosis may be difficult. With no specific symptoms, cardiac sarcoidosis may be difficult to suspect in a patient with no previous extra-cardiac sarcoidosis diagnosis. This manuscript reviews the current knowledge of the diagnosis and decision to treat cardiac sarcoidosis, and illustrates the information with a case presentation of a young adult with no risk factors, no previous diagnosis of sarcoidosis, and with cardiac symptoms impairing his quality of life.
A minireview on the bioremediative potential of microbial enzymes as solution to emerging microplastic pollution
Authors: De Jesus Rener , Alkendi Ruwaya
Published: March 2023

Accumulating plastics in the biosphere implicates adverse effects, raising serious concern among scientists worldwide. Plastic waste in nature disintegrates into microplastics. Because of their minute appearance, at a scale of <5 mm, microplastics easily penetrate different pristine water bodies and terrestrial niches, posing detrimental effects on flora and fauna. The potential bioremediative application of microbial enzymes is a sustainable solution for the degradation of microplastics. Studies have reported a plethora of bacterial and fungal species that can degrade synthetic plastics by excreting plastic-degrading enzymes. Identified microbial enzymes, such as IsPETase and IsMHETase from Ideonella sakaiensis 201-F6 and Thermobifida fusca cutinase (Tfc), are able to depolymerize plastic polymer chains producing ecologically harmless molecules like carbon dioxide and water. However, thermal stability and pH sensitivity are among the biochemical limitations of the plastic-degrading enzymes that affect their overall catalytic activities. The application of biotechnological approaches improves enzyme action and production. Protein-based engineering yields enzyme variants with higher enzymatic activity and temperature-stable properties, while site-directed mutagenesis using the Escherichia coli model system expresses mutant thermostable enzymes. Furthermore, microalgal chassis is a promising model system for “green” microplastic biodegradation. Hence, the bioremediative properties of microbial enzymes are genuinely encouraging for the biodegradation of synthetic microplastic polymers.
Influence of pressurization rate and mode on cell damage of Escherichia coli and Staphyloccocus aureus by high hydrostatic pressure
Authors: Yang Dong , Li Renjie , Dong Peng , Rao Lei , Wang Yongtao , Liao Xiaojun
Published: March 2023

As a non-thermal technology, high hydrostatic pressure (HHP) has been widely investigated for inactivating microorganisms in food. Few studies have been presented on the pressurization/depressurization rate and mode of microbial inactivation. In this study, effect of pressurization rate and mode on Escherichia coli and Staphylococcus aureus cell damage during HHP treatment was investigated. The results showed that fast pressurization + linear mode (FL) treatment has the best bactericidal effect on E. coli and S. aureus, followed by fast pressurization + stepwise mode (FS) and slow pressurization + stepwise mode (SS) treatments. FL treatment caused more morphological damage to the cell wall, cell membrane, and cytoplasmic components compared with FS and SS treatment detected by SEM and TEM. Additionally, the damage to membrane permeability of them was also enhanced after FL treatment. Therefore, our results indicated that FL treatment could be applied to enhance the bactericidal effect of HHP on bacteria by increasing the damage to cell morphological structure and membrane integrity.
Hydrogen peroxide treatment induces the transposition of an insertion sequence in Deinococcus radiopugnans DY59
Authors: Shin Eunjung , Noh Hee Seong , Ye Qianying , Lee Sung-Jae
Published: March 2023

Deinococcus radiopugnans DY59 (formerly Deinococcus swuensis DY59) is a radiation-resistant bacterium isolated from soil. From the 3.5 Mb genomic DNA sequence of strain DY59 (December 2014), 31 insertion sequence (IS) elements of six IS families including IS1, IS4, IS5, IS66, IS630, and IS701 and five unclassified IS elements were detected. Upon induction of oxidative stress with 80 and 100 mM H2O2, the unique ISs of the IS4 family member were actively translocated into a carotenoid biosynthesis gene phytoene desaturase (QR90_10400), resulting in non-pigment phenotypic selection. Therefore, these active transpositions of a specific IS family member were induced by oxidative stress at 80 and 100 mM H2O2. Furthermore, D. radiopugnans DY59 exhibited extremely higher MIC values against H2O2 treatment. To explain this phenomenon, qRT-PCR was conducted to assess the expression levels of catalase and three LysR family regulators. Our findings indicated that the ISDrpg2 and ISDrpg3 elements of the IS4 family were actively transposed into the phytoene desaturase gene by H2O2 treatment via replicative transposition. However, high H2O2 resistance did not originate from H2O2-induced expression of catalase and LysR family regulators.
Stronger linkage of diversity-carbon decomposition for rare rather than abundant bacteria in woodland soils
Authors: Cao Hui , Li Suying , He Huan , Sun Yaoqin , Wu Yichao , Huang Qiaoyun , Cai Peng , Gao Chun-Hui
Published: March 2023

Soil microbial diversity is important for maintaining ecosystem functions. However, the linkage between microbial diversity, especially rare and abundant bacterial diversity, and carbon decomposition remains largely unknown. In this study, we assessed the establishment and maintenance of rare and abundant bacterial α-diversities at the taxonomic and phylogenetic levels and their linkages with soil carbon decomposition separately in four Chinese woodlands. Compared to abundant bacteria, rare bacteria showed higher community diversity, tighter phylogenetic clustering, wider environmental breadth, stronger phylogenetic signals, and higher functional redundancy. The assembly of the abundant bacterial subcommunity was governed by stochastic (59.2%) and deterministic (41.8%) processes, whereas the assembly of the rare bacterial subcommunity was mainly dominated by deterministic processes (85.8%). Furthermore, total phosphorus, soil pH, and ammonium nitrogen balanced stochastic and deterministic processes in both rare and abundant bacterial subcommunities. Our results reveal that rare bacteria displayed stronger environmental adaptability and environmental constraint. Importantly, the α-diversities of rare taxa, rather than abundant taxa, were significantly related to carbon decomposition. This study provides a holistic understanding of biogeographic patterns of abundant and rare bacteria and their α-diversities in relation to carbon decomposition, thus helping us better predict and regulate carbon dynamics under the background of global climate change.
Transcriptome analysis of the biofilm formation mechanism of Vibrio parahaemolyticus under the sub-inhibitory concentrations of copper and carbenicillin
Authors: Xie Jiaying , Zhang Hongmin , Li Yinhui , Li Hao , Pan Yingjie , Zhao Yong , Xie Qingchao
Published: March 2023

Biofilm formation of Vibrio parahaemolyticus enhanced its tolerance to the environment, but caused many serious problems to food safety and human health. In this paper, the effects of copper and carbenicillin (CARB) stress on the formation of the biofilms of V. parahaemolyticus organisms were studied, and RNA sequencing technology was used to compare the differences in transcriptome profiles of the biofilm-related genes of V. parahaemolyticus organisms under different sub-inhibitory stresses. The results proved that V. parahaemolyticus had a large growth difference under the two stresses, copper and CARB at 1/2 minimal inhibitory concentration (MIC), and it could form a stable biofilm under both stress conditions. The amount of biofilm formed under CARB stress was significantly higher than that of copper stress (p < 0.05). Based on the analysis of transcriptome sequencing results 323, 1,550, and 1,296 significantly differential expressed genes were identified in the three treatment groups namely 1/2 MIC CARB, Cu2+, and Cu2++CARB. Through COG annotation, KEGG metabolic pathway analysis and gene expression analysis related to biofilm formation, the functional pathways of transcriptome changes affecting V. parahaemolyticus were different in the three treatment groups, and the CARB treatment group was significantly different from the other two groups. These differences indicated that the ABC transport system, two-component system and quorum sensing were all involved in the biofilm formation of the V. parahaemolytic by regulating flagellar motility, extracellular polysaccharides and extracellular polymer synthesis. Exploring the effects of different stress conditions on the transcriptome of V. parahaemolyticus could provide a basis for future research on the complex network system that regulates the formation of bacterial biofilms.
Role of the subthalamic nucleus in perceiving and estimating the passage of time
Authors: Honma Motoyasu , Sasaki Fuyuko , Kamo Hikaru , Nuermaimaiti Maierdanjiang , Kujirai Hitoshi , Atsumi Takeshi , Umemura Atsushi , Iwamuro Hirokazu , Shimo Yasushi , Oyama Genko , Hattori Nobutaka , Terao Yasuo
Published: March 2023

Sense of time (temporal sense) is believed to be processed by various brain regions in a complex manner, among which the basal ganglia, including the striatum and subthalamic nucleus (STN), play central roles. However, the precise mechanism for processing sense of time has not been clarified. To examine the role of the STN in temporal processing of the sense of time by directly manipulating STN function by switching a deep brain stimulation (DBS) device On/Off in 28 patients with Parkinson’s disease undergoing STN-DBS therapy. The test session was performed approximately 20 min after switching the DBS device from On to Off or from Off to On. Temporal sense processing was assessed in three different tasks (time reproduction, time production, and bisection). In the three temporal cognitive tasks, switching STN-DBS to Off caused shorter durations to be produced compared with the switching to the On condition in the time production task. In contrast, no effect of STN-DBS was observed in the time bisection or time reproduction tasks. These findings suggest that the STN is involved in the representation process of time duration and that the role of the STN in the sense of time may be limited to the exteriorization of memories formed by experience.
The block design subtest of the Wechsler adult intelligence scale as a possible non-verbal proxy of cognitive reserve
Authors: Corujo-Bolaños Graciela , Yánez-Pérez Roraima , Cedrés Nira , Ferreira Daniel , Molina Yaiza , Barroso José
Published: March 2023

Objectives

To investigate the potential of the Block design subtest of the Wechsler Adults Intelligence Scale as a non-verbal proxy of cognitive reserve.

Method

A total of 391 cognitively unimpaired participants were included in this study. The association between the Block design subtest and the Information subtest (an established verbal proxy of cognitive reserve) from the WAIS, as well as the association of the two subtests with a Cognitive Reserve Questionnaire (CRQ) were tested. In addition, multiple linear regression models were conducted to investigate the association of the Block design and Information subtests with cognitive performance. The capacity of the Block design subtest to minimize the negative effect of an older age over cognitive performance was also assessed and this effect was compared with that of the Information subtest. The four cognitive domains included were: verbal memory, visual–visuospatial memory, executive-premotor functions and processing speed.

Results

The Block design subtest correlated positively with both the Information subtest and the CRQ. A statistically significant association was observed between the Block design subtest and all four cognitive domains. Higher scores in the Block design subtest minimized the negative effect of aging on the cognitive domains of visual–visuospatial memory and executive-premotor functions, in a similar way to the results obtained for the Information subtest.

Conclusion

The Block design subtest is significantly correlated with two established proxies of cognitive reserve: it correlates with cognitive performance and high scores in Block design have the capacity to minimize the negative effect of an older age on cognitive performance. Therefore, the results suggest that the corrected Block design subtest could be considered as a non-verbal proxy of cognitive reserve.
Ethanol combined with energy drinks: Two decades of research in rodents
Authors: Petribu Beatriz Nunes , Abrahao Karina Possa , Souza-Formigoni Maria Lucia Oliveira
Published: March 2023

Many studies raised concerns on alcoholic beverages consumption mixed with energy drinks (AmED), which can induce higher rates of binge drinking and earlier development of alcohol use disorders. After 20 years of research, few studies with laboratory animals have focused on the effects of this mixture and the neurobiological and pharmacological mechanisms underlying them. We found 16 articles on AmED administration to rodents evaluating its effects on voluntary consumption, locomotion, anxiety-like behavior, memory, influence on the onset time of seizures, biochemical and neurochemical measures. Some of these studies indicated energy drinks (ED) can alter the pattern of use and motivation to consume ethanol (EtOH); increase the expression of sensitization to EtOH stimulant effect and the proportion of sensitized mice; decrease the aversiveness of high concentrations of EtOH, among other effects. In addition AmED hastens the loss of righting reflex and its effects on memory are controversial. After acute administration no difference was found in blood ethanol concentration (BEC) of rodents which received EtOH with or without ED, but after 60 days of treatment, AmED group had lower BEC levels than EtOH group. Data on biochemical and neurochemical parameters after AmED are not consistent. Although the AmED group presented higher glucose levels than the EtOH group when drugs were administered by gavage, this was not observed in a self-administration protocol. AmED may induce higher kidney damage, higher levels of plasma urea, uric acid and creatinine when compared to EtOH. Chronic consumption of AmED causes an inflammatory response and oxidative stress, which may induce cell death in the cortex and hypothalamus of adult rats. These controversial results show that AmED diverse effects depend on sex, age and lineage of the animals, duration of the treatment and route of administration. Further research is necessary to evaluate the mechanisms underlying AmED biological effects.
Excessive rest time during active phase is reliably detected in a mouse model of myotonic dystrophy type 1 using home cage monitoring
Authors: Golini Elisabetta , Rigamonti Mara , Raspa Marcello , Scavizzi Ferdinando , Falcone Germana , Gourdon Genevieve , Mandillo Silvia
Published: March 2023

Myotonic dystrophy type 1 (DM1) is a dominantly inherited neuromuscular disease caused by the abnormal expansion of CTG-repeats in the 3′-untranslated region of the Dystrophia Myotonica Protein Kinase (DMPK) gene, characterized by multisystemic symptoms including muscle weakness, myotonia, cardio-respiratory problems, hypersomnia, cognitive dysfunction and behavioral abnormalities. Sleep-related disturbances are among the most reported symptoms that negatively affect the quality of life of patients and that are present in early and adult-onset forms of the disease. DMSXL mice carry a mutated human DMPK transgene containing >1,000 CTGrepeats, modeling an early onset, severe form of DM1. They exhibit a pathologic neuromuscular phenotype and also synaptic dysfunction resulting in neurological and behavioral deficits similar to those observed in patients. Additionally, they are underweight with a very high mortality within the first month after birth presenting several welfare issues. To specifically explore sleep/rest-related behaviors of this frail DM1 mouse model we used an automated home cage-based system that allows 24/7 monitoring of their activity non-invasively. We tested male and female DMSXL mice and their wild-type (WT) littermates in Digital Ventilated Cages (DVCR) assessing activity and rest parameters on day and night for 5 weeks. We demonstrated that DMSXL mice show reduced activity and regularity disruption index (RDI), higher percentage of zero activity per each hour and longer periods of rest during the active phase compared to WT. This novel rest-related phenotype in DMSXL mice, assessed unobtrusively, could be valuable to further explore mechanisms and potential therapeutic interventions to alleviate the very common symptom of excessive daytime sleepiness in DM1 patients.
Advances in transcorneal electrical stimulation: From the eye to the brain
Authors: Agadagba Stephen K. , Lim Lee Wei , Chan Leanne Lai Hang
Published: March 2023

The mammalian brain is reported to contain about 106–109 neurons linked together to form complex networks. Physiologically, the neuronal networks interact in a rhythmic oscillatory pattern to coordinate the brain’s functions. Neuromodulation covers a broad range of techniques that can alter neuronal network activity through the targeted delivery of electrical or chemical stimuli. Neuromodulation can be used to potentially treat medical conditions and can serve as a research tool for studying neural functions. Typically, the main method of neuromodulation is to electrically stimulate specific structures in both the central and peripheral nervous systems via surgically implanted electrodes. Therefore, it is imperative to explore novel and safer methods for altering neuronal network activity. Transcorneal electrical stimulation (TES) has rapidly emerged as a non-invasive neuromodulatory technique that can exert beneficial effects on the brain through the eyes. There is substantial evidence to show that TES can change the brain oscillations in rodents. Moreover, the molecular data clearly shows that TES can also activate non-visual brain regions. In this review, we first summarize the use of TES in the retina and then discuss its effects in the brain through the eye-brain connection. We then comprehensively review the substantial evidence from electrophysiological, behavioral, and molecular studies on the role of TES on modulating neurons in the brain. Lastly, we discuss the implications and possible future directions of the research on TES as a non-invasive tool for neuromodulation of the brain via directly stimulating the mammalian eye.
Efficacy and safety of low dose aspirin plus clopidogrel in the treatment of elderly patients with symptomatic intracranial artery stenosis
Authors: Song Hai-xia , Zhang Bin , Liu Shan , Shi Zhi-chao , Wang Zi-yun , Lu Hai-li , Yao Jie , Chen Juan
Published: March 2023

Background

As one of the most common causes of stroke, symptomatic intracranial artery stenosis (sICAS) is a great threat to public health, and its financial burden is substantial, with annual direct high medical costs particularly in China. Currently, the long-term use of conventional dual antiplatelet therapy (DAPT) as the primary modality of treatment for sICAS decreases the risk of stroke recurrence but increases the risk of bleeding. We aimed to evaluate the efficacy and safety of low dose aspirin plus clopidogrel for the treatment of sICAS in the elderly population.

Methods

This randomized, controlled study included 181 older patients with transient ischemic attack (TIA) or ischemic stroke (IS) attributed to sICAS, who were recruited between April 2015 and November 2020. The 90 patients assigned to the low dose therapy group included aspirin, 75 mg, plus clopidogrel, 50 mg, daily for 90 and 91 patients assigned to the conventional group included aspirin, 100 mg, plus clopidogrel, 75 mg, daily for 90 days (aspirin or clopidogrel alone daily thereafter) were included in this intention-to-treat analysis. Efficacy and safety analyses were done in this trial.

Results

One hundred eighty-one eligible elderly patients with sICAS were enrolled in this trial. The median age was 70 years ranged 60–83 years. Seventy-five participants were with TIA and 106 with IS. The median time of follow-up was 30 months ranged 1–36 months. Ninety patients were assigned randomly to the low dose group and 91 patients to the conventional group. The rate of primary, secondary and composite efficacy were not significantly different between the low dose and conventional group (P > 0.05). The rate of composite safety outcome was 7.8% (7/90) in the low dose group, which was lower than 17.6% (16/91) in the conventional group (χ2 = 3.921, P = 0.048). At the time of last follow-up, 17 (9.4%) of 181 patients developed GI injuries, which occurred in four (4.4%) of 90 patients in the low dose group and in 13 (14.3%) of 91 patients in the conventional group (χ2 = 4.058, P = 0.044). The primary efficacy outcome occurred in six (18.2%) of 33 patients with severe sICAS and in 22 (38.6%) of 57 patients with moderate sICAS (χ2 = 4.064, P = 0.044) in the low dose group.

Conclusion

In this study, the safety of low dose aspirin combined with clopidogrel proved to be equally efficient and significantly safer than those of conventional dose within 24 months in elderly patients with sICAS. However, the small size of this study limits the validity of the results. Further larger longitudinal and randomized controlled trials are necessary to evaluate the role of low dose DAPT in the patients with sICAS.
Multilingual evaluation of interpretable biomarkers to represent language and speech patterns in Parkinson's disease
Authors: Favaro Anna , Moro-Velázquez Laureano , Butala Ankur , Motley Chelsie , Cao Tianyu , Stevens Robert David , Villalba Jesús , Dehak Najim
Published: March 2023

Motor impairments are only one aspect of Parkinson's disease (PD), which also include cognitive and linguistic impairments. Speech-derived interpretable biomarkers may help clinicians diagnose PD at earlier stages and monitor the disorder's evolution over time. This study focuses on the multilingual evaluation of a composite array of biomarkers that facilitate PD evaluation from speech. Hypokinetic dysarthria, a motor speech disorder associated with PD, has been extensively analyzed in previously published studies on automatic PD evaluation, with a relative lack of inquiry into language and task variability. In this study, we explore certain acoustic, linguistic, and cognitive information encoded within the speech of several cohorts with PD. A total of 24 biomarkers were analyzed from American English, Italian, Castilian Spanish, Colombian Spanish, German, and Czech by conducting a statistical analysis to evaluate which biomarkers best differentiate people with PD from healthy participants. The study leverages conceptual robustness as a criterion in which a biomarker behaves the same, independent of the language. Hence, we propose a set of speech-based biomarkers that can effectively help evaluate PD while being language-independent. In short, the best acoustic and cognitive biomarkers permitting discrimination between experimental groups across languages were fundamental frequency standard deviation, pause time, pause percentage, silence duration, and speech rhythm standard deviation. Linguistic biomarkers representing the length of the narratives and the number of nouns and auxiliaries also provided discrimination between groups. Altogether, in addition to being significant, these biomarkers satisfied the robustness requirements.
Mind wandering in reading: An embodied approach
Authors: Trasmundi Sarah Bro , Toro Juan
Published: March 2023

In the last 20 years, the study of mind wandering has attracted the attention of a growing number of researchers from fields like psychology, philosophy, and neuroscience. Mind wandering has been characterized in multiple ways: as task-unrelated, unintentional, stimulus-independent, or unguided thought processes. Those accounts have mostly focused on the identification of neurocognitive mechanisms that enable the emergence of mind-wandering episodes. Reading is one activity in which mind wandering frequently occurs, and it is widely accepted that mind wandering is detrimental for reading flow, comprehension and the capacity to make inferences based on the text. This mind wandering scepsis in reading is based on two unchallenged views: (i) that reading is a disembodied, mental activity of information processing, and (ii) that mind wandering is essentially characterized as a task-unrelated and involuntary thought process that disrupts all kinds of goal-oriented behavior. However, recent developments within cognitive science treat the mind as embodied and thus challenge both ontological and epistemological assumptions about what mind wandering is, where it is located, and how it is being studied empirically during reading. In this article we integrate embodied accounts of mind wandering and reading to show how reading benefits from nested mind wandering processes. Empirically, we investigate how a reader can move successfully in and out of different embodied processes and mesh different cognitive strategies over time, including some forms of mind wandering. While such changes in reading are frequently deemed dysfunctional, we suggest an alternative interpretation: Rather than seeking constant flow and fluency, we propose that reading is multi-actional and benefits from drawing on different cognitive strategies spanning mind wandering processes and goal-oriented behavior. In that sense, we suggest that mind wandering has a potential for enriching cognitive processes underlying reading, such as imagining and reflection. We exemplify these insights through analyses of data obtained in ethnographic and semi-experimental studies of reading practices. We conclude that to capture cognitive phenomena within an embodied framework, a richer methodology must be developed. Such a methodology must not only be capable of accounting for brains, bodies, and contexts in isolation, but must consider an overall brain-body-environment system.