bluuebunny/crossref_metadata_2025 huggingface dataset:
SECURITIZATION OF SEARCH AND RESCUE AT SEA: THE RESPONSE TO BOAT MIGRATION IN THE MEDITERRANEAN AND OFFSHORE AUSTRALIA
Authors: Ghezelbash Daniel , Moreno-Lax Violeta , Klein Natalie , Opeskin Brian
Published: January 2018

This article compares the law and practice of the European Union and Australia in respect to the search and rescue (SAR) of boat migrants, concluding that the response to individuals in peril at sea in both jurisdictions is becoming increasingly securitized. This has led to the humanitarian purpose of SAR being compromised in the name of border security. Part I contrasts the unique challenge posed by SAR operations involving migrants and asylum seekers, as opposed to other people in distress at sea. Part II analyses the relevant international legal regime governing SAR activities and its operation among European States and in offshore Australia. Part III introduces the securitization framework as the explanatory paradigm for shifting State practice and its impact in Europe and Australia. It then examines the consequences of increasing securitization of SAR in both jurisdictions and identifies common trends, including an increase in militarization and criminalization, a lack of transparency and accountability, developments relating to disembarkation andnon-refoulement, and challenges relating to cooperation and commodification.
Are Ratings the Worst Form of Credit Assessment Except for All the Others?
Authors: Blöchlinger Andreas , Leippold Markus
Published: January 2018

We present a prediction model to forecast corporate defaults. In a theoretical model, under incomplete information in a market with publicly traded equity, we show that our approach must outperform ratings, Altman’sZ-score, and Merton’s distance to default. We reconcile the statistical and structural approaches under a common framework; that is, our approach nests Altman’s and Merton’s approaches as special cases. Empirically, the combined approach is indeed the most powerful predictor, and the numbers of observed defaults align well with the estimated probabilities. With a new transformation method, we obtain cycle-adjusted forecasts that still outperform ratings.
The Medieval Space: Early Medieval Documents as Stages
Authors: Thomas Kyle A.
Published: January 2018

Peter Brook begins the second chapter ofThe Empty Space,“The Holy Theatre,” with a lament for the loss of sacred approaches to theatre; approaches that satisfy a community's need to make visible its identity, its hope, and its history. In describing the vacuum within the modern theatre once occupied by ceremony—what he defines as the importance of a noble aim for theatre—Brook critiques hollow and backward attempts to fill new and grand spaces with old and meaningless ritual. In postwar Europe, he saw a need for new spaces that “crie[d] out for a new ceremony, but of course it is the new ceremony that should have come first—it is the ceremony in all its meanings that should have dictated the shape of the place.” Brook's assessment of postwar European bourgeois theatre and its search for new and meaningful agendas is framed by conceptions of space as antecedent to action, requiring only performer and audience in order for theatre to occur, and for a space to be called a theatre. Indeed, theatrical space is always a product of well-established cultural performance conventions—a phenomenon common throughout history. Brook's critique focuses on the conventions of theatrical space that developed from the romantic dramas and spectacle-driven performances of the late nineteenth century and continued well into the twentieth century. Echoing Bertolt Brecht, Brook rejected theatres that predetermined the limits of drama and performance, arguing that it was necessary to strip them of conventional expectations in order to lay bare their potential. Essentially, he asks: When and how does a space become a theatre?
Islands, the Humanities and environmental conservation
Authors: TROMPF GARRY W.
Published: January 2018

This paper concerns itself with the contributions that the Humanities make to the understanding of islands and their bettered environmental conservation. Most distinctively, the Humanities comprise Literary Studies, Studies in Art and Culture (including Indigenous and Gender Studies) and Philosophy (with Aesthetics and the History of Ideas), but they also encompass Archaeology, History, Linguistics, Studies in Religion and, of late, Media and Communication Studies, even though members of this latter cluster frequently deploy methods from the social sciences. The goal here is to explore many of the implications such Human Studies and their sub-branches may have for island conservation, above all informed by the History of Ideas, in order to introduce the relevant key issues and inter-relationships and offer the most judicious illustrative materials. Variances in the reach and special attention of all these branches of knowledge are vast and intricate, while complex relativities apply both in the types of island situations and in expectations about what can or should be conserved. Since the mass of apposite discussions in the literature cannot possibly be summarized here, this article circumvents the difficulties by means of a special double-edged review. It ranges over the history of human consciousness of insular worlds, as reflected in mythic, legendary and historical materials, yet en route it uncovers how Humanities research can elucidate the human responses to islands through known time and shows how developing meaning-making has generally enhanced the appeal of sea-locked environments as worth conserving.
Replicating Writing: Moulding and Stamping Hieroglyphs on Classic Maya Ceramics
Authors: Matsumoto Mallory E.
Published: January 2018

A fundamental distinction is made in craft production between custom or bespoke creation and mechanical reproduction that generates multiple iterations of the same form. In Mesoamerica, technologies of reproduction are attested by around the sixth century bc in the form of moulding and stamping, and they become increasingly common in ceramic production in the Maya and neighbouring regions in the third or fourth century. Beginning in the Late Classic period (c. 600–830 ad), Maya artisans applied them to the hieroglyphic script as well, generating a corpus of texts that are at once fundamentally distinct from and intimately linked to the broader scribal tradition dominated by hand-written texts. This article examines Classic Maya texts moulded and stamped on ceramics in the context of scribal practice and the social and cultural role of the script. I argue that these artefacts manifest changes not only in hieroglyphic production, but also in writing's role in user communities. Consequentially, they invite reconsideration of scribal practice's relationship to other crafting traditions, as well as the diversity of modes of engaging in Classic Maya scribal tradition.
Mothering and Gender Equality in Iceland: Irreconcilable Opposites?
Authors: Gíslason Ingólfur V. , Símonardóttir Sunna
Published: January 2018

Iceland enjoys a reputation as one of the most gender equal countries in the world. It has also received much attention for an innovative approach to parental leave where fathers have three months of non-transferable leave, thereby encouraging active involvement of fathers in the caretaking of their children. This article focuses on the discrepancy between on the one hand the goals of the state of drawing men, particularly fathers, into traditional female dominated areas such as caregiving of infants and young children and on the other hand a discourse that equates motherhood with parenthood and promotes the ideology of intensive mothering.
Is freedom (still) therapy? The 40th anniversary of the Italian mental health care reform
Authors: Fioritti A.
Published: January 2018

On 13 May 1978, the Italian Parliament approved Law 180, universally known as ‘Basaglia Law’ after the name of the leader of the anti-institutional movement which promoted this radical community mental health care reform. Forty years later, Italian psychiatry still runs a community care system, albeit with degrees of solidity and quality very varied along the peninsula. Mental health care is still an integral part of the National Health System, with liberal regulations on coercion and a lowest number of general hospital and residential facilities beds. Recently, Italy has also closed the special forensic psychiatric institutions and brought the care of the mentally ill offenders within the responsibilities of local Mental Health Departments. Over time, psychiatric deinstitutionalisation inspired policies in other sectors of Italian society, such as those regarding physical and intellectual disabilities, education of children with special needs, drug addictions and management of deviant minors. Furthermore, debate about Law 180 has reached and maintained an international dimension, becoming a term of reference for international agencies such as the World Health Organization and the European Commission, for good and for evil. The overall balance sheet of the Reform process would seem mostly positive, though the last decade has seen many threats challenging the system. Mental health care services have been asked to do much more, in terms of care to a larger population with very diversified needs, but with much less resources, due to the financial consequences of the economic crisis. Although there is no evidence of a trend towards re-institutionalisation, intensity and quality of care may have fallen below acceptable standards in some parts of Italy.
Haemostaseological complication management in caval and iliac venous stenting
Authors: Kohler Corinne , Fuss Torsten , Schweizer Ronny , Baumgartner Iris , Kucher Nils , Schindewolf Marc
Published: January 2018

Abstract. Antiplatelet and anticoagulation therapy after venous stenting is still not standardized, data from randomized-controlled trials are missing. Rare prothrombotic disorders and nonresponsiveness to drugs must be taken into account. This case report demonstrates successful haemostaselogical complication management in recurrent rethromboses due to underlying clopidogrel resistance and low responsiveness to anticoagulation with dabigatran after endovascular stent reconstruction of chronic pelvic and caval vein occlusion in a patient with severe postthrombotic syndrome.
Rechenprobleme von Grundschulkindern: Persistenz und Schulerfolg nach fünf Jahren
Authors: Schulz Franziska , Wyschkon Anne , Gallit Finja S. , Poltz Nadine , Moraske Svenja , Kucian Karin , von Aster Michael , Esser Günter
Published: January 2018

Zusammenfassung.Fragestellung: Ziel war die Untersuchung des Verlaufs von Kindern mit Rechenstörungen bzw. Rechenschwächen. Neben der Persistenz wurden Auswirkungen von Rechenproblemen auf künftige Rechenleistungen sowie den Schulerfolg geprüft. Methodik: Für 2909 Schüler der 2. bis 5. Klasse liegen die Resultate standardisierter Rechen- und Intelligenztests vor. Ein Teil dieser Kinder ist nach 37 und 68 Monaten erneut untersucht worden. Ergebnisse: Die Prävalenz von Rechenstörungen betrug 1.4%, Rechenschwächen traten bei 11.2% auf. Rechenprobleme zeigten eine mittlere bis hohe Persistenz. Schüler mit Rechenschwäche blieben im Rechnen gut eine Standardabweichung hinter durchschnittlich und ca. eine halbe Standardabweichung hinter unterdurchschnittlich intelligenten Kontrollkindern zurück. Der allgemeine Schulerfolg rechenschwacher Probanden (definiert über Mathematiknote, Deutschnote und Schultyp) ähnelte dem der unterdurchschnittlich intelligenten Kontrollgruppe und blieb hinter dem Schulerfolg durchschnittlich intelligenter Kontrollkinder zurück. Eingangs ältere Probanden mit Rechenproblemen (4. bis 5. Klasse) wiesen eine schlechtere Prognose auf als Kinder, die zu Beginn die 2. oder 3. Klasse besuchten. Schlussfolgerungen: Rechenprobleme stellen ein ernsthaftes Entwicklungsrisiko dar. Längsschnittuntersuchungen, die Kinder mit streng definierter Rechenstörung bis ins Erwachsenenalter begleiten und Prädiktoren für unterschiedlich erfolgreiche Verläufe ermitteln, sind dringend notwendig.
Transsensibilität statt Diskriminierung
Authors: van Peeren Nadine
Published: January 2018

Zusammenfassung. Menschen mit einer Transidentität stellen besondere Anforderungen an die Hilfesysteme. Wenig transsensible Hilfen können großen Schaden anrichten und werden oft als Diskriminierung erlebt.
Beziehungsgestaltung in der Zusammenarbeit mit Menschen mit sexuellen Gewalterfahrungen
Authors: Sauter Dorothea , Rixe Jacqueline
Published: January 2018

Zusammenfassung. Sexualisierte Gewalt hat viele Gesichter und reicht von einmaligen verbalen Übergriffen bis hin zu langjährigen erzwungenen sexuellen Handlungen, kombiniert mit anderen Formen von Gewaltausübung. Dieser Beitrag fokussiert auf Menschen, die in der Kindheit schwerem sexuellen Missbrauch ausgesetzt waren. Beschrieben werden Aspekte der Beziehungsgestaltung, die in der psychiatrischen Versorgung von Menschen mit Missbrauchserfahrun- gen zu berücksichtigen sind; auf die vielfältigen Hilfe- und Therapiekonzepte wird nicht eingegangen.
Aufklärung und Auswege finden
Authors: Benz Jutta , Loureiro Prati Michael
Published: January 2018

Zusammenfassung. In der Kinder- und Jugendpsychiatrie, behandeln wir sowohl Heranwachsende, die in ihrem sozialen Umfeld sexuell auffällig geworden sind, als auch diejenigen, die bereits zum Zeitpunkt der Aufnahme, erwiesen gegen die sexuelle Selbstbestimmung Anderer, gehandelt haben. Hierbei bewegen wir uns in einem Spektrum von sexuell belästigendem Verhalten, bis hin zu schweren Formen der Vergewaltigung.
Psychiatrie Inklusiv
Authors: Ott-Ordelheide Petra
Published: January 2018

Zusammenfassung. „Der besondere Patient“, so werden Menschen mit Behinderungen und psychischen Störungen sowohl in der Presse, im Internet aber auch in der Pflegepraxis beschrieben. Diese Aussage suggeriert, dass es sich um ein besonders seltenes Phänomen handelt. Dabei ist die geistige Entwicklung bei 320 000 Menschen die Ursache für die Anerkennung des Status der Schwerbehinderung (Statistisches Bundesamt, 2015). Fast alle Menschen mit Behinderungen haben weitere Komorbiditäten, hier sind vor allen Dingen orthopädische Probleme, Epilepsie und körperliche Behinderungen zu nennen. Rund 20 % haben eine psychiatrische Komorbidität (Seidel, 2005).
HOXC8 promotes proliferation and migration through transcriptional up-regulation of TGFβ1 in non-small cell lung cancer
Authors: Liu Houli , Zhang Mingsheng , Xu Shanshan , Zhang Jie , Zou Jin , Yang Chenchen , Zhang Yang , Gong Chen , Kai Yuanzhong , Li Yong
Published: January 2018

Homeobox (HOX) genes encode a family of transcription factors, which play crucial roles in numerous processes, and their dysregulation is involved in the carcinogenesis of many human cancers. In the present study, we investigated the roles of HOXC8 in non-small cell lung cancer (NSCLC). We showed that HOXC8 was upregulated in clinical NSCLC specimens compared to normal lung tissues, and the high expression of HOXC8 correlated with tumor node metastasis (TNM) stage, tumor status, lymph nodal status and poor relapse-free survival for lung cancer patients. Functionally, HOXC8 expression significantly promoted the proliferation, anchorage-independent growth and migration of NSCLC, and HOXC8 functioned as a transcription activator to induce the expression of TGFβ1, leading to an increase in the proliferation, anchorage-independent growth and migration of NSCLC. Furthermore, we demonstrated that HOXC8 expression was associated with chemoresistance and anti-apoptosis in NSCLC, suggesting that HOXC8 is a promising therapeutic target for chemosensitization of NSCLC to cisplatin. Altogether, our study defined a critical role of HOXC8 in promoting transcription of TGFβ1 and NSCLC tumorigenesis.
Calling from distance: attraction of soil bacteria by plant root volatiles
Authors: Schulz-Bohm Kristin , Gerards Saskia , Hundscheid Maria , Melenhorst Jasper , de Boer Wietse , Garbeva Paolina
Published: January 2018

Plants release a wide set of secondary metabolites including volatile organic compounds (VOCs). Many of those compounds are considered to function as defense against herbivory, pests, and pathogens. However, little knowledge exists about the role of belowground plant VOCs for attracting beneficial soil microorganisms. We developed an olfactometer system to test the attraction of soil bacteria by VOCs emitted by Carex arenaria roots. Moreover, we tested whether infection of C. arenaria with the fungal pathogen Fusarium culmorum modifies the VOCs profile and bacterial attraction. The results revealed that migration of distant bacteria in soil towards roots can be stimulated by plant VOCs. Upon fungal infection, the blend of root VOCs changed and specific bacteria with antifungal properties were attracted. Tests with various pure VOCs indicated that those compounds can diffuse over long distance but with different diffusion abilities. Overall, this work highlights the importance of plant VOCs in belowground long-distance plant–microbe interactions.
Anti-Hermitian photodetector facilitating efficient subwavelength photon sorting
Authors: Kim Soo Jin , Kang Ju-Hyung , Mutlu Mehmet , Park Joonsuk , Park Woosung , Goodson Kenneth E. , Sinclair Robert , Fan Shanhui , Kik Pieter G. , Brongersma Mark L.
Published: January 2018

The ability to split an incident light beam into separate wavelength bands is central to a diverse set of optical applications, including imaging, biosensing, communication, photocatalysis, and photovoltaics. Entirely new opportunities are currently emerging with the recently demonstrated possibility to spectrally split light at a subwavelength scale with optical antennas. Unfortunately, such small structures offer limited spectral control and are hard to exploit in optoelectronic devices. Here, we overcome both challenges and demonstrate how within a single-layer metafilm one can laterally sort photons of different wavelengths below the free-space diffraction limit and extract a useful photocurrent. This chipscale demonstration of anti-Hermitian coupling between resonant photodetector elements also facilitates near-unity photon-sorting efficiencies, near-unity absorption, and a narrow spectral response (∼ 30 nm) for the different wavelength channels. This work opens up entirely new design paradigms for image sensors and energy harvesting systems in which the active elements both sort and detect photons.
Orientation of non-spherical protonated water clusters revealed by infrared absorption dichroism
Authors: Daldrop Jan O. , Saita Mattia , Heyden Matthias , Lorenz-Fonfria Victor A. , Heberle Joachim , Netz Roland R.
Published: January 2018

Infrared continuum bands that extend over a broad frequency range are a key spectral signature of protonated water clusters. They are observed for many membrane proteins that contain internal water molecules, but their microscopic mechanism has remained unclear. Here we compute infrared spectra for protonated and unprotonated water chains, discs, and droplets from ab initio molecular dynamics simulations. The continuum bands of the protonated clusters exhibit significant anisotropy for chains and discs, with increased absorption along the direction of maximal cluster extension. We show that the continuum band arises from the nuclei motion near the excess charge, with a long-ranged amplification due to the electronic polarizability. Our experimental, polarization-resolved light–dark difference spectrum of the light-driven proton pump bacteriorhodopsin exhibits a pronounced dichroic continuum band. Our results suggest that the protonated water cluster responsible for the continuum band of bacteriorhodopsin is oriented perpendicularly to the membrane normal.
Publisher Correction: The low affinity neurotrophin receptor CD271 regulates phenotype switching in melanoma
Authors: Restivo Gaetana , Diener Johanna , Cheng Phil F. , Kiowski Gregor , Bonalli Mario , Biedermann Thomas , Reichmann Ernst , Levesque Mitchell P. , Dummer Reinhard , Sommer Lukas
Published: January 2018

The originally published version of this Article was updated shortly after publication to add the words ‘The’ and ‘affinity’ to the title, following their inadvertent removal during the production process. This has now been corrected in both the PDF and HTML versions of the Article.
The cross correlation properties of composite systems
Authors: Huang Zhifu , Zheng Shuqing
Published: January 2018

A new method is presented for characterizing cross correlations in composite systems described by a couple of time-dependent random variables. This method is based on (i) rescaling the time derivatives of the variables to make their variances unity and then (ii) recombining these rescaled variables into their sum and difference. This manipulation enables one to express the joint probability distribution function in a peculiar way. It is also found that the entropy of composite systems is not equal to the sum of entropy of each subsystem because of the cross correlations.
Insights into cerebral haemodynamics and oxygenation utilising in vivo mural cell imaging and mathematical modelling
Authors: Sweeney Paul W. , Walker-Samuel Simon , Shipley Rebecca J.
Published: January 2018

The neurovascular mechanisms underpinning the local regulation of cerebral blood flow (CBF) and oxygen transport remain elusive. In this study we have combined novel in vivo imaging of cortical microvascular and mural cell architecture with mathematical modelling of blood flow and oxygen transport, to provide new insights into CBF regulation that would be inaccessible in a conventional experimental context. Our study indicates that vasoconstriction of smooth muscle actin-covered vessels, rather than pericyte-covered capillaries, induces stable reductions in downstream intravascular capillary and tissue oxygenation. We also propose that seemingly paradoxical observations in the literature around reduced blood velocity in response to arteriolar constrictions might be caused by a propagation of constrictions to upstream penetrating arterioles. We provide support for pericytes acting as signalling conduits for upstream smooth muscle activation, and erythrocyte deformation as a complementary regulatory mechanism. Finally, we caution against the use of blood velocity as a proxy measurement for flow. Our combined imaging-modelling platform complements conventional experimentation allowing cerebrovascular physiology to be probed in unprecedented detail.
Structural basis of thalidomide enantiomer binding to cereblon
Authors: Mori Tomoyuki , Ito Takumi , Liu Shujie , Ando Hideki , Sakamoto Satoshi , Yamaguchi Yuki , Tokunaga Etsuko , Shibata Norio , Handa Hiroshi , Hakoshima Toshio
Published: January 2018

Thalidomide possesses two optical isomers which have been reported to exhibit different pharmacological and toxicological activities. However, the precise mechanism by which the two isomers exert their different activities remains poorly understood. Here, we present structural and biochemical studies of (S)- and (R)-enantiomers bound to the primary target of thalidomide, cereblon (CRBN). Our biochemical studies employed deuterium-substituted thalidomides to suppress optical isomer conversion, and established that the (S)-enantiomer exhibited ~10-fold stronger binding to CRBN and inhibition of self-ubiquitylation compared to the (R)-enantiomer. The crystal structures of the thalidomide-binding domain of CRBN bound to each enantiomer show that both enantiomers bind the tri-Trp pocket, although the bound form of the (S)-enantiomer exhibited a more relaxed glutarimide ring conformation. The (S)-enantiomer induced greater teratogenic effects on fins of zebrafish compared to the (R)-enantiomer. This study has established a mechanism by which thalidomide exerts its effects in a stereospecific manner at the atomic level.
Author Correction: CODA: Integrating multi-level context-oriented directed associations for analysis of drug effects
Authors: Yu Hasun , Jung Jinmyung , Yoon Seyeol , Kwon Mijin , Bae Sunghwa , Yim Soorin , Lee Jaehyun , Kim Seunghyun , Kang Yeeok , Lee Doheon
Published: January 2018

A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper.
NMR studies on lignocellulose deconstructions in the digestive system of the lower termite Coptotermes formosanus Shiraki
Authors: Tarmadi Didi , Tobimatsu Yuki , Yamamura Masaomi , Miyamoto Takuji , Miyagawa Yasuyuki , Umezawa Toshiaki , Yoshimura Tsuyoshi
Published: January 2018

Termites represent one of the most efficient lignocellulose decomposers on earth. The mechanism by which termites overcome the recalcitrant lignin barrier to gain access to embedded polysaccharides for assimilation and energy remains largely unknown. In the present study, softwood, hardwood, and grass lignocellulose diets were fed to Coptotermes formosanus workers, and structural differences between the original lignocellulose diets and the resulting feces were examined by solution-state multidimensional nuclear magnetic resonance (NMR) techniques as well as by complementary wet-chemical methods. Overall, our data support the view that lignin polymers are partially decomposed during their passage through the termite gut digestive system, although polysaccharide decomposition clearly dominates the overall lignocellulose deconstruction process and the majority of lignin polymers remain intact in the digestive residues. High-resolution NMR structural data suggested preferential removal of syringyl aromatic units in hardwood lignins, but non-acylated guaiacyl units as well as tricin end-units in grass lignins. In addition, our data suggest that termites and/or their gut symbionts may favor degradation of C–C-bonded β–5 and resinol-type β–β lignin inter-monomeric units over degradation of ether-bonded β–O–4 units, which is in contrast to what has been observed in typical lignin biodegradation undertaken by wood-decaying fungi.
Precision of a new ocular biometer in children and comparison with IOLMaster
Authors: Yu Xinxin , Chen Hao , Savini Giacomo , Zheng Qianqian , Song Benhao , Tu Ruixue , Huang Jinhai , Wang Qinmei
Published: January 2018

To assess the repeatability and reproducibility of AL-Scan in agreement with those by the IOLMaster in healthy children, two skilled operators measured ocular parameters in 58 children. The parameters included keratometry (K) values, anterior chamber depth (ACD), axial length (AL), central corneal thickness (CCT), pupil diameter (PD), and corneal diameter (CD). The cohort comprised of 32 boys and 26 girls. The AL-Scan measurements showed high repeatability, as the test-retest repeatability (TRT) values of AL, CCT, ACD, Kf, Ks, Km, CD, and PD were 0.09 mm, 5.1 μm, 0.04 mm, 0.28 D, 0.24 D, 0.21 D, 0.39 mm, and 0.22 mm, respectively. The within-subject coefficient of variation (CoV) was low (<0.35%) and the intraclass correlation coefficients (ICC) of all parameters were >0.85. The interobserver reproducibility was excellent with low values of TRT and ICC > 0.95. The CoV of AL, CCT, ACD, and K was <0.22%. The 95% limits of agreement between the AL-Scan and the IOLMaster were narrow for all parameters except for CD. The repeatability and reproducibility of the new biometer, Al-Scan, was excellent for all parameters and can be routinely used in children to measure the biometric values.
Echocardiographic validation of pulmonary hypertension due to heart failure with reduced ejection fraction in mice
Authors: Dayeh Nour R. , Tardif Jean-Claude , Shi Yanfen , Tanguay Mégane , Ledoux Jonathan , Dupuis Jocelyn
Published: January 2018

Pulmonary hypertension (PH) associated with left heart diseases is the most prevalent cause of PH. The scarcity of studies exploring the pathophysiology and therapies of group II PH resides in the lack of validated small animal models with non-invasive determination of the presence and severity of PH. Heart failure (HF) was induced in mice by coronary artery ligation. Mice developed PH as evidenced by an elevated right ventricular (RV) systolic pressure and RV hypertrophy. Detailed non-invasive echocardiographic analysis on the left and right ventricles showed impaired left ventricular (LV) systolic and diastolic function. In addition, RV hypertrophy was confirmed by echo and accompanied by impaired function as well as increased pulmonary resistance. Correlation analysis validated the use of the LV wall-motion score index (WMSI) at a threshold value of ≥2.0 as a powerful and reliable indicator for the presence of PH and RV dysfunction. Echocardiography is an accurate non-invasive technique to diagnose PH in a HF mouse model. Moreover, an echocardiographic parameter of infarct size and LV function, the LV WMSI, reliably correlates with the presence of PH, RV hypertrophy and RV dysfunction and could be used to improve efficiency and design of pre-clinical studies.
Lithospheric folding by flexural slip in subduction zones as source for reverse fault intraslab earthquakes
Authors: Romeo I. , Álvarez-Gómez J. A.
Published: January 2018

Subduction requires the permanent generation of a bend fold in the subducting slab which mechanics is not well understood. Lithospheric bending of subducting slabs was traditionally considered to be accommodated by orthogonal flexure, generating extensional outer rise earthquakes responsible of the external arc elongation during folding. Here we explore the possibility of lithospheric flexure being accommodated through simple shear deformation parallel to the slab (folding by flexural slip) and evaluate this process as source of earthquakes. The seismicity predicted by flexural slip dominated slab bending explains a significant amount of intermediate earthquakes observed in subduction zones with different degrees of coupling. This mechanism predicts the generation of intraslab thrust earthquakes with fault planes subparallel to the slab top. Being the orientations of the fault planes the same for the interface thrust earthquakes and the flexural-slip intraslab earthquakes, the amount of seismic moment liberated by the interface could be significantly lower than considered before. This proposed seismic source should be taken into account in models and hazard studies of subduction zones. Determining the seismic generating processes in subduction zones and their characteristics is a fundamental issue for the correct assessment of the associated seismic and tsunami risk.
The prevalence and treatment pattern of clinically diagnosed pelvic organ prolapse: a Korean National Health Insurance Database-based cross-sectional study 2009–2015
Authors: Yuk Jin-Sung , Lee Jung Hun , Hur Jun-Young , Shin Jung-Ho
Published: January 2018

The study aim was to evaluate the prevalence of pelvic organ prolapse using claim data of South Korea and to evaluate treatment patterns. The Korea National Health Insurance Corporation pay medical costs for most diseases. This study used Health Insurance Review & Assessment Service-National Inpatient Sample (HIRA-NIS) 2009–2015. Pelvic organ prolapse was defined by diagnostic code (N81.x). Of the approximately 4.5 million women included in HIRA-NIS 2009–2015, 10,305 women were selected as having pelvic organ prolapse, and the mean age of the pelvic organ prolapse group was 63.9 ± 0.2 years. The prevalence of pelvic organ prolapse was 180 ± 4 per 100,000 population in women older than 50 years old. In logistic regression analysis, constipation increased the prevalence of all pelvic organ prolapse (odds ratio, 4.04; 95% confidence interval, 3.52–4.63; P < 0.01). The number of women requiring pessary only and surgery only were 26 ± 2 per 100,000 population and 89 ± 1 per 100,000 population, respectively, for women over 50 years of age. The prevalence of pelvic organ prolapse was quite lower than in previous studies. Surgery peaked at approximately 70 years of age. Pessary increased dramatically among women after the age of 65.
Gender-specific associations of skeletal muscle mass and arterial stiffness among peritoneal dialysis patients
Authors: Liu Xinhui , Zheng Xunhua , Yi Chunyan , Wu Juan , Ye Hongjian , Guo Qunying , Yu Xueqing , Yang Xiao
Published: January 2018

Decreased skeletal muscle has been identified as a relevant factor for arterial stiffness but has not been thoroughly investigated in peritoneal dialysis (PD) patients. The aim of this study was to investigate the relationship between skeletal muscle and arterial stiffness in PD patients. A cross-sectional study of 658 prevalent PD patients with a mean brachial-ankle pulse wave velocity (baPWV) of 1714 (±501) cm/s and mean skeletal muscle mass of 26.6 (±5.4) kg was performed. Skeletal muscle mass level was significantly higher in males than in females. When examining skeletal muscle mass as a continuous variable, skeletal muscle mass was significantly associated with baPWV in fully adjusted linear regression models in total patients [standardized coefficients (β), −0.181; 95% confidence interval (95% CI), −0.276 to −0.056; P = 0.003] or female patients (β, −0.119; 95% CI, −0.350 to −0.015; P = 0.03) but not in male patients (β, −0.117; 95% CI, −0.300 to 0.011; P = 0.07). Furthermore, in females, a significant association between the middle or highest tertile of skeletal muscle mass and baPWV was found in fully adjusted models (β, −0.123; 95% CI, −0.204 to −0.008; P = 0.03; β, −0.140; 95% CI, −0.228 to −0.016; P = 0.02, respectively). In conclusion, decreased skeletal muscle mass was independently associated with increased baPWV in PD patients, and this association was significant in females but not in males.
Aversive gustatory learning and perception in honey bees
Authors: Guiraud Marie , Hotier Lucie , Giurfa Martin , de Brito Sanchez María Gabriela
Published: January 2018

Taste perception allows discriminating edible from non-edible items and is crucial for survival. In the honey bee, the gustatory sense has remained largely unexplored, as tastants have been traditionally used as reinforcements rather than as stimuli to be learned and discriminated. Here we provide the first characterization of antennal gustatory perception in this insect using a novel conditioning protocol in which tastants are dissociated from their traditional food-reinforcement role to be learned as predictors of punishment. We found that bees have a limited gustatory repertoire via their antennae: they discriminate between broad gustatory modalities but not within modalities, and are unable to differentiate bitter substances from water. Coupling gustatory conditioning with blockade of aminergic pathways in the bee brain revealed that these pathways are not restricted to encode reinforcements but may also encode conditioned stimuli. Our results reveal unknown aspects of honey bee gustation, and bring new elements for comparative analyses of gustatory perception in animals.
Large room-temperature tunneling anisotropic magnetoresistance and electroresistance in single ferromagnet/Nb:SrTiO3 Schottky devices
Authors: Kamerbeek Alexander M. , Ruiter Roald , Banerjee Tamalika
Published: January 2018

There is a large effort in research and development to realize electronic devices capable of storing information in new ways - for instance devices which simultaneously exhibit electro and magnetoresistance. However it remains a challenge to create devices in which both effects coexist. In this work we show that the well-known electroresistance in noble metal-Nb:SrTiO3 Schottky junctions can be augmented by a magnetoresistance effect in the same junction. This is realized by replacing the noble metal electrode with ferromagnetic Co. This magnetoresistance manifests as a room temperature tunneling anisotropic magnetoresistance (TAMR). The maximum room temperature TAMR (1.6%) is significantly larger and robuster with bias than observed earlier, not using Nb:SrTiO3. In a different set of devices, a thin amorphous AlOx interlayer inserted between Co and Nb:SrTiO3, reduces the TAMR by more than 2 orders of magnitude. This points to the importance of intimate contact between the Co and Nb:SrTiO3 for the TAMR effect. This is explained by electric field enhanced spin-orbit coupling of the interfacial Co layer in contact with Nb:SrTiO3. We propose that the large TAMR likely has its origin in the 3d orbital derived conduction band and large relative permittivity of Nb:SrTiO3 and discuss ways to further enhance the TAMR.
Establishment of an easy and straight forward heparinase protocol to analyse circulating and myocardial tissue micro-RNA during coronary artery-bypass-graft surgery
Authors: Engler Andrea , Dreja Florian , Köberle Sarah , Thielmann Matthias , Peters Jürgen , Frey Ulrich H.
Published: January 2018

Coronary artery-bypass-graft (CABG) surgery is associated with myocardial damage and increased blood concentrations of circulating microRNAs (miRNA). However, whether and to what extent these miRNAs relate to cardiac tissue miRNA expression have not yet been explored. Since plasma miRNA quantification in samples from cardiopulmonary bypass (CPB) patients is severely hampered by heparin, we established and validated successfully a protocol to reliably measure miRNA in 49 heparinized patients undergoing CABG so as to investigate the relationship between circulating and right atrial miRNAs. Plasma and right atrial expression of miR-1, miR-133a, miR-423-5p, and miR-499 were measured before and after CPB, as well as miRNAs in plasma 24 h thereafter. All plasma miRNAs increased significantly with surgery while cardiac tissue expression of only miR-133a (1.4-fold; p = 0.003) and miR-423-5p (1.3 fold; p = 0.025) increased as well. Right atrial and plasma miR-133a expression correlated positively before CPB (r = 0.288, p = 0.045) but miR-499 expression inversely (r = −0.484, p = 0.0004). There was a strong association between plasma miR-133a and miR-499 concentrations and postoperative troponin I concentrations, the marker for myocardial damage. Increased myocardial miR-133a and miR-423-5p expression together with unchanged miR-1 and miR-499 expression might suggest active release of these miRNAs rather than their origin from damaged cells.
Production of Wilson Disease Model Rabbits with Homology-Directed Precision Point Mutations in the ATP7B Gene Using the CRISPR/Cas9 System
Authors: Jiang Weihua , Liu Lili , Chang Qiurong , Xing Fengying , Ma Zhengwen , Fang Zhenfu , Zhou Jing , Fu Li , Wang Huiyang , Huang Xingxu , Chen Xuejin , Li Yao , Li Shangang
Published: January 2018

CRISPR/Cas9 has recently been developed as an efficient genome engineering tool. The rabbit is a suitable animal model for studies of metabolic diseases. In this study, we generated ATP7B site-directed point mutation rabbits to simulate a major mutation type in Asians (p. Arg778Leu) with Wilson disease (WD) by using the CRISPR/Cas9 system combined with single-strand DNA oligonucleotides (ssODNs). The efficiency of the precision point mutation was 52.94% when zygotes were injected 14 hours after HCG treatment and was significantly higher than that of zygotes injected 19 hours after HCG treatment (14.29%). The rabbits carrying the allele with mutant ATP7B died at approximately three months of age. Additionally, the copper content in the livers of rabbits at the onset of WD increased nine-fold, a level similar to the five-fold increase observed in humans with WD. Thus, the efficiency of precision point mutations increases when RNAs are injected into zygotes at earlier stages, and the ATP7B mutant rabbits are a potential model for human WD disease with applications in pathological analysis, clinical treatment and gene therapy research.
New functions of Semaphorin 3E and its receptor PlexinD1 during developing and adult hippocampal formation
Authors: Mata Agata , Gil Vanessa , Pérez-Clausell Jeús , Dasilva Miguel , González-Calixto Mari Carmen , Soriano Eduardo , García-Verdugo José Manuel , Sanchez-Vives Maria V. , del Río José Antonio
Published: January 2018

The development and maturation of cortical circuits relies on the coordinated actions of long and short range axonal guidance cues. In this regard, the class 3 semaphorins and their receptors have been seen to be involved in the development and maturation of the hippocampal connections. However, although the role of most of their family members have been described, very few data about the participation of Semaphorin 3E (Sema3E) and its receptor PlexinD1 during the development and maturation of the entorhino-hippocampal (EH) connection are available. In the present study, we focused on determining their roles both during development and in adulthood. We determined a relevant role for Sema3E/PlexinD1 in the layer-specific development of the EH connection. Indeed, mice lacking Sema3E/PlexinD1 signalling showed aberrant layering of entorhinal axons in the hippocampus during embryonic and perinatal stages. In addition, absence of Sema3E/PlexinD1 signalling results in further changes in postnatal and adult hippocampal formation, such as numerous misrouted ectopic mossy fibers. More relevantly, we describe how subgranular cells express PlexinD1 and how the absence of Sema3E induces a dysregulation of the proliferation of dentate gyrus progenitors leading to the presence of ectopic cells in the molecular layer. Lastly, Sema3E mutant mice displayed increased network excitability both in the dentate gyrus and the hippocampus proper.
Λ-enhanced grey molasses on the D2 transition of Rubidium-87 atoms
Authors: Rosi Sara , Burchianti Alessia , Conclave Stefano , Naik Devang S. , Roati Giacomo , Fort Chiara , Minardi Francesco
Published: January 2018

Laser cooling based on dark states, i.e. states decoupled from light, has proven to be effective to increase the phase-space density of cold trapped atoms. Dark-states cooling requires open atomic transitions, in contrast to the ordinary laser cooling used for example in magneto-optical traps (MOTs), which operate on closed atomic transitions. For alkali atoms, dark-states cooling is therefore commonly operated on the D1 transition nS1/2 → nP1/2. We show that, for 87Rb, thanks to the large hyperfine structure separations the use of this transition is not strictly necessary and that “quasi-dark state” cooling is efficient also on the D2 line, 5S1/2 → 5P3/2. We report temperatures as low as (4.0 ± 0.3) μK and an increase of almost an order of magnitude in the phase space density with respect to ordinary laser sub-Doppler cooling.
Deactivation of Cellulase at the Air-Liquid Interface Is the Main Cause of Incomplete Cellulose Conversion at Low Enzyme Loadings
Authors: Bhagia Samarthya , Dhir Rachna , Kumar Rajeev , Wyman Charles E.
Published: January 2018

Amphiphilic additives such as bovine serum albumin (BSA) and Tween have been used to improve cellulose hydrolysis by cellulases. However, there has been a lack of clarity to explain their mechanism of action in enzymatic hydrolysis of pure or low-lignin cellulosic substrates. In this work, a commercial Trichoderma reesei enzyme preparation and the amphiphilic additives BSA and Tween 20 were applied for hydrolysis of pure Avicel cellulose. The results showed that these additives only had large effects on cellulose conversion at low enzyme to substrate ratios when the reaction flasks were shaken. Furthermore, changes in the air-liquid interfacial area profoundly affected cellulose conversion, but surfactants reduced or prevented cellulase deactivation at the air-liquid interface. Not shaking the flasks or adding low amounts of surfactant resulted in near theoretical cellulose conversion at low enzyme loadings given enough reaction time. At low enzyme loadings, hydrolysis of cellulose in lignocellulosic biomass with low lignin content suffered from enhanced enzyme deactivation at the air-liquid interface.
Physical basis of specificity and delayed binding of a subtype selective sodium channel inhibitor
Authors: Corry Ben
Published: January 2018

Nerve and muscle signalling is controlled by voltage-gated sodium (Nav) channels which are the targets of local anesthetics, anti-epileptics and anti-arrythmics. Current medications do not selectively target specific types of Nav found in the body, but compounds that do so have the potential to be breakthrough treatments for chronic pain, epilepsy and other neuronal disorders. We use long computer simulations totaling more than 26 μs to show how a promising lead compound can target one Nav implicated in pain perception and specific channels found in bacteria, and accurately predict the affinity of the compound to different channel types. Most importantly, we provide two explanations for the slow kinetics of this class of compound that limits their therapeutic utility. Firstly, the negative charge on the compound is essential for high affinity binding but is also responsible for energetic barriers that slow binding. Secondly, the compound has to undergo a conformational reorientation during the binding process. This knowledge aids the design of compounds affecting specific eukaryotic and bacterial channels and suggests routes for future drug development.
Spin orbit torques induced magnetization reversal through asymmetric domain wall propagation in Ta/CoFeB/MgO structures
Authors: Cao Jiangwei , Chen Yifei , Jin Tianli , Gan Weiliang , Wang Ying , Zheng Yuqiang , Lv Hua , Cardoso Susana , Wei Dan , Lew Wen Siang
Published: January 2018

The magnetization reversal induced by spin orbit torques in the presence of Dzyaloshinskii-Moriya interaction (DMI) in perpendicularly magnetized Ta/CoFeB/MgO structures were investigated by using a combination of Anomalous Hall effect measurement and Kerr effect microscopy techniques. By analyzing the in-plane field dependent spin torque efficiency measurements, an effective field value for the DMI of ~300 Oe was obtained, which plays a key role to stabilize Néel walls in the film stack. Kerr imaging reveals that the current-induced reversal under small and medium in-plane field was mediated by domain nucleation at the edge of the Hall bar, followed by asymmetric domain wall (DW) propagation. However, as the in-plane field strength increases, an isotropic DW expansion was observed before reaching complete reversal. Micromagnetic simulations of the DW structure in the CoFeB layer suggest that the DW configuration under the combined effect of the DMI and the external field is responsible for the various DW propagation behaviors.
Synthetic gene regulation for independent external induction of the Saccharomyces cerevisiae pseudohyphal growth phenotype
Authors: Pothoulakis Georgios , Ellis Tom
Published: January 2018

Pseudohyphal growth is a multicellular phenotype naturally performed by wild budding yeast cells in response to stress. Unicellular yeast cells undergo gross changes in their gene regulation and elongate to form branched filament structures consisting of connected cells. Here, we construct synthetic gene regulation systems to enable external induction of pseudohyphal growth in Saccharomyces cerevisiae. By controlling the expression of the natural PHD1 and FLO8 genes we are able to trigger pseudohyphal growth in both diploid and haploid yeast, even in different types of rich media. Using this system, we also investigate how members of the BUD gene family control filamentation in haploid cells. Finally, we employ a synthetic genetic timer network to control pseudohyphal growth and further explore the reversibility of differentiation. Our work demonstrates that synthetic regulation can exert control over a complex multigene phenotype and offers opportunities for rationally modifying the resulting multicellular structure.