bluuebunny/crossref_metadata_2025 huggingface dataset:
ELK3 expressed in lymphatic endothelial cells promotes breast cancer progression and metastasis through exosomal miRNAs
Authors: Kim Kwang-Soo , Park Ji-In , Oh Nuri , Cho Hyeon-Ju , Park Ji-Hoon , Park Kyung-Soon
Published: June 2019

Tumor-associated lymphatic vessels (LV) serve as a route of cancer dissemination through the prometastatic crosstalk between lymphatic endothelial cells (LECs) lining the LVs and cancer cells. Compared to blood endothelial cell-derived angiocrine factors, however, LEC-secreted factors in the tumor microenvironment and their roles in tumor metastasis are poorly understood. Here, we report that ELK3 expressed in LECs contributes to the dissemination of cancer cells during tumor growth by providing oncogenic miRNAs to tumor cells through exosomes. We found that conditioned medium from ELK3-suppressed LECs (LCM) lost its ability to promote the migration and invasion of breast cancer cells such as MDA-MB-231, Hs578T and BT20 in vitro. Suppression of ELK3 in LECs diminished the ability of LECs to promote tumor growth and metastasis of MDA-MB-231 in vivo. Exosomes derived from LECs significantly increased the migration and invasion of MDA-MB-231 in vitro, but ELK3 suppression significantly diminished the pro-oncogenic activity of exosomes from LECs. Based on the miRNA expression profiles of LECs and functional analysis, we identified miR-503-3p, miR-4269 and miR-30e-3p as downstream targets of ELK3 in LECs, which cause the above phenotype of cancer cells. These findings strongly suggest that ELK3 expressed in LECs is a major regulator that controls the communication between the tumor microenvironment and tumors to support cancer metastasis.
Overexpression of MAP3K3 promotes tumour growth through activation of the NF-κB signalling pathway in ovarian carcinoma
Authors: Zhang Ying , Wang Sha-Sha , Tao Lin , Pang Li-Juan , Zou Hong , Liang Wei-Hua , Liu Zheng , Guo Su-Liang , Jiang Jin-Fang , Zhang Wen-Jie , Jia Wei , Li Feng
Published: June 2019

Mitogen-activated protein kinase kinase kinase 3 (MAP3K3), a member of the serine/threonine protein kinase family, is ubiquitously expressed and acts as an oncogene. However, the expression and exact molecular mechanism of MAP3K3 in ovarian carcinoma (OC) remain unclear. Here, we found that MAP3K3 protein was highly expressed in 70.5% of high-grade serous ovarian carcinoma (HGSOC) samples. MAP3K3 overexpression was significantly associated with the FIGO stage and chemotherapy response. Additionally, MAP3K3 overexpression was associated with reduced disease-free survival and overall survival. In vitro experiments showed that MAP3K3 overexpression promoted cell proliferation, inhibited apoptosis, and enhanced the migration and invasion of OC cells. Moreover, in vivo tumourigenesis experiments confirmed that silencing MAP3K3 significantly reduced the growth rate and volume of transplanted tumours in nude mice. Drug sensitivity experiments demonstrated that differential expression of MAP3K3 in OC cell lines correlates with chemotherapy resistance. Functionally, the MAP3K3 gene regulated the malignant biological behaviour of OC cells by mediating NF-κB signalling pathways, affecting the downstream epithelial-mesenchymal transition and cytoskeletal protein expression. Our results unveiled the role of MAP3K3 in mediating NF-κB signalling to promote the proliferation, invasion, migration, and chemotherapeutic resistance of OC cells, highlighting a potential new therapeutic and prognostic target.
Two phase kinetics of the inflammatory response from hepatocyte-peripheral blood mononuclear cell interactions
Authors: Beringer Audrey , Molle Jennifer , Bartosch Birke , Miossec Pierre
Published: June 2019

Active liver diseases are characterized by an infiltration of inflammatory immune cells, which interact locally with hepatocytes. Co-cultures between non- and -activated human peripheral blood mononuclear cells (PBMCs) and human hepatoma HepaRG cells were used to determine the role of these cell interactions in the inflammatory response. At the early stage, PBMC-HepaRG cell interactions increased mRNA expression and/or secretion of IL-6, IL-8, CCL-20 and MCP-1, in part through direct cell contact and the induction was higher in PHA-activated conditions. The pro-inflammatory cytokines IL-17 and/or TNFα contributed to the increase of IL-6 and IL-8 secretion. HepaRG cells modulated T cell polarization by increasing Th1 cell transcription factor expression and by reducing CD3+ CD4+ IL-17+ cell frequency when PBMCs were activated with PHA. At a later stage, the presence of HepaRG cells inhibited PHA-induced HLA-DR expression on PBMCs, and PBMC proliferation. In contrast, the presence of skin fibroblasts had no effect of PBMC proliferation induced by PHA. After a first pro-inflammatory phase, PBMC-HepaRG cell interactions may down-regulate the immune response. The PBMC-hepatocyte interactions can thus participate first to the initiation of hepatitis and later to the maintenance of immune tolerance in liver, possibly contributing to chronicity.
Reciprocal cortical activation patterns during incisal and molar biting correlated with bite force levels: an fMRI study
Authors: Yoshizawa Hideyuki , Miyamoto Jun J. , Hanakawa Takashi , Shitara Hitoshi , Honda Manabu , Moriyama Keiji
Published: June 2019

In humans, the incisors and molars have distinct functions during mastication, analogous to the two main types of handgrip, the precision and power grips. In the present study, we investigated cortical activation and masticatory muscle activity during incisal and molar biting via simultaneous functional magnetic resonance imaging and electromyogram (EMG) recordings. We conducted recordings in 15 healthy adult participants while they performed incisal and molar biting tasks at three step-wise force levels using two custom-made splints. Regarding the results of the ROI analysis, we found a significantly stronger positive linear correlation between the blood oxygenation level dependent signal and EMG activity during molar biting than incisal biting, which was particularly prominent in the primary sensorimotor cortex and the cerebellum. We also found a significantly stronger negative linear correlation during incisal biting than molar biting, which was particularly prominent in the rostral cingulate motor area, superior frontal gyrus, and caudate nucleus. These findings indicate that molar biting enables powerful chewing: brain activity in several brain areas related to motor function was increased with increasing bite force levels, while incisal biting enables fine motor control: brain activity in several brain areas related to motor control was increased with reduced bite force levels.
Identification of reliable reference genes for qRT-PCR in the ephemeral plant Arabidopsis pumila based on full-length transcriptome data
Authors: Jin Yuhuan , Liu Fang , Huang Wei , Sun Qi , Huang Xianzhong
Published: June 2019

Arabidopsis pumila, an annual ephemeral plant, plays important roles in preventing wind and sand erosion, water and soil conservation, and microhabitat improvement in the North of Xinjiang, China. Studies of adaptive mechanisms in harsh desert environments at the genetic and genomic levels can be used to more effectively develop and protect this species. The quantitative real-time polymerase chain reaction (qRT-PCR) method is one of the essential means to achieve these goals, and the selection of an appropriate reference gene is the prerequisite for qRT-PCR. In this study, 10 candidate reference genes were identified from the full-length transcriptome data of A. pumila, and their expression stabilities under four abiotic stresses (drought, heat, cold and salt) and in seven different tissues (roots, hypocotyl, cotyledon, leaves, stems, flowers and siliques) were evaluated with four programmes geNorm, NormFinder, Bestkeeper and RefFinder. Although the most stable reference genes were variable under different treatments using different software, comprehensive ranking revealed that UEP and HAF1 under drought stress, UBQ9 and GAPDH under heat stress, UBC35 and GAPDH under cold stress, GAPDH and ACT1 under salt stress, and ACT1 and GAPDH in different tissues were the most stable reference genes. Moreover, GAPDH and UBQ9 were the most suitable reference gene combinations for all samples. The expression pattern of the K+ uptake permease gene KUP9 further validated that the selected reference genes were suitable for normalization of gene expression. The identification of reliable reference genes guarantees more accurate qRT-PCR quantification for A. pumila and facilitates functional genomics studies of ephemeral plants.
Diagnostic role of medical thoracoscopy in childhood pleural tuberculosis
Authors: Wang Maoshui , Han Chao , He Yu
Published: June 2019

Currently, the diagnostic role of medical thoracoscopy in childhood pleural tuberculosis remains uncertain. Therefore, this retrospective study was conducted to evaluate the diagnostic performance of histological examination of tissue samples obtained by medical thoracoscopy in childhood pleural tuberculosis. Hospitalized children who underwent medical thoracoscopy between May 2012 and March 2016 were included in the study. Tissue samples obtained by thoracoscopy were submitted for histological examination (hematoxylin and eosin staining). Descriptive statistical methods were used for data interpretation, and the data were expressed as the mean ± standard deviation. The childhood tuberculosis patients had the following characteristics: 11 had pleural tuberculosis and 9 had pleural tuberculosis + pulmonary tuberculosis, the average age was 13.0 ± 2.2 years old, 60% were male, 26.3% (5/19) of patients tested positive for acid-fast bacilli positive in smears, 21.1% (4/19) of patients were positive for TB-PCR, and 90% (18) of pleural tuberculosis patients were positive in the culture for Mycobacterium tuberculosis. The sensitivity of histological examinations of tissue samples obtained by thoracoscopy in the detection of pleural tuberculosis was 80% (16/20). Complications were reported in 15 cases, and all complications disappeared spontaneously without any specific treatment. Therefore, we concluded that medical thoracoscopy was a sensitive and safe tool for the detection of childhood pleural tuberculosis.
Brain activity sustaining the modulation of pain by empathetic comments
Authors: Fauchon C. , Faillenot I. , Quesada C. , Meunier D. , Chouchou F. , Garcia-Larrea L. , Peyron R.
Published: June 2019

Empathetic verbal feedback from others has been shown to alleviate the intensity of experimental pain. To investigate the brain changes associated with this effect, we conducted 3T-fMRI measurements in 30 healthy subjects who received painful thermal stimuli on their left hand while overhearing empathetic, neutral or unempathetic comments, supposedly made by experimenters, via headsets. Only the empathetic comments significantly reduced pain intensity ratings. A whole-brain BOLD analysis revealed that both Empathetic and Unempathetic conditions significantly increased the activation of the right anterior insular and posterior parietal cortices to pain stimuli, while activations in the posterior cingulate cortex and precuneus (PCC/Prec) were significantly stronger during Empathetic compared to Unempathetic condition. BOLD activity increased in the DLPFC in the Empathetic condition and decreased in the PCC/Prec and vmPFC in the Unempathetic condition. In the Empathetic condition only, functional connectivity increased significantly between the vmPFC and the insular cortex. These results suggest that modulation of pain perception by empathetic feedback involves a set of high-order brain regions associated with autobiographical memories and self-awareness, and relies on interactions between such supra-modal structures and key nodes of the pain system.
Pleomorphic Adenoma Gene 1 Is Needed For Timely Zygotic Genome Activation and Early Embryo Development
Authors: Madissoon Elo , Damdimopoulos Anastasios , Katayama Shintaro , Krjutškov Kaarel , Einarsdottir Elisabet , Mamia Katariina , De Groef Bert , Hovatta Outi , Kere Juha , Damdimopoulou Pauliina
Published: June 2019

Pleomorphic adenoma gene 1 (PLAG1) is a transcription factor involved in cancer and growth. We discovered a de novo DNA motif containing a PLAG1 binding site in the promoters of genes activated during zygotic genome activation (ZGA) in human embryos. This motif was located within an Alu element in a region that was conserved in the murine B1 element. We show that maternally provided Plag1 is needed for timely mouse preimplantation embryo development. Heterozygous mouse embryos lacking maternal Plag1 showed disrupted regulation of 1,089 genes, spent significantly longer time in the 2-cell stage, and started expressing Plag1 ectopically from the paternal allele. The de novo PLAG1 motif was enriched in the promoters of the genes whose activation was delayed in the absence of Plag1. Further, these mouse genes showed a significant overlap with genes upregulated during human ZGA that also contain the motif. By gene ontology, the mouse and human ZGA genes with de novo PLAG1 motifs were involved in ribosome biogenesis and protein synthesis. Collectively, our data suggest that PLAG1 affects embryo development in mice and humans through a conserved DNA motif within Alu/B1 elements located in the promoters of a subset of ZGA genes.
Sequence and expression analysis of HSP70 family genes in Artemia franciscana
Authors: Junprung Wisarut , Norouzitallab Parisa , De Vos Stephanie , Tassanakajon Anchalee , Nguyen Viet Dung , Van Stappen Gilbert , Bossier Peter
Published: June 2019

Thus far, only one gene from the heat shock protein 70 (HSP70) family has been identified in Artemia franciscana. Here, we used the draft Artemia transcriptome database to search for other genes in the HSP70 family. Four novel HSP70 genes were identified and designated heat shock cognate 70 (HSC70), heat shock 70 kDa cognate 5 (HSC70-5), Immunoglobulin heavy-chain binding protein (BIP), and hypoxia up-regulated protein 1 (HYOU1). For each of these genes, we obtained nucleotide and deduced amino acid sequences, and reconstructed a phylogenetic tree. Expression analysis revealed that in the juvenile state, the transcription of HSP70 and HSC70 was significantly (P < 0.05) higher in a population of A. franciscana selectively bred for increased induced thermotolerance (TF12) relative to a control population (CF12). Following non-lethal heat shock treatment at the nauplius stage, transcription of HSP70, HSC70, and HSC70-5 were significantly (P < 0.05) up-regulated in TF12. In contrast, transcription of the other HSP70 family members in A. franciscana (BIP, HYOU1, and HSPA4) showed no significant (P > 0.05) induction. Gene expression analysis demonstrated that not all members of the HSP70 family are involved in the response to heat stress and selection and that especially altered expression of HSC70 plays a role in a population selected for increased thermotolerance.
Morph-specific protein patterns in the femoral gland secretions of a colour polymorphic lizard
Authors: Mangiacotti Marco , Fumagalli Marco , Cagnone Maddalena , Viglio Simona , Bardoni Anna Maria , Scali Stefano , Sacchi Roberto
Published: June 2019

Colour polymorphism occurs when two or more genetically-based colour morphs permanently coexist within an interbreeding population. Colouration is usually associated to other life-history traits (ecological, physiological, behavioural, reproductive …) of the bearer, thus being the phenotypic marker of such set of genetic features. This visual badge may be used to inform conspecifics and to drive those decision making processes which may contribute maintaining colour polymorphism under sexual selection context. The importance of such information suggests that other communication modalities should be recruited to ensure its transfer in case visual cues were insufficient. Here, for the first time, we investigated the potential role of proteins from femoral gland secretions in signalling colour morph in a polymorphic lizard. As proteins are thought to convey identity-related information, they represent the ideal cues to build up the chemical modality used to badge colour morphs. We found strong evidence for the occurrence of morph-specific protein profiles in the three main colour-morphs of the common wall lizard, which showed both qualitative and quantitative differences in protein expression. As lizards are able to detect proteins by tongue-flicking and vomeronasal organ, this result support the hypothesis that colour polymorphic lizards may use a multimodal signal to inform about colour-morph.
Sequence and structural patterns detected in entangled proteins reveal the importance of co-translational folding
Authors: Baiesi Marco , Orlandini Enzo , Seno Flavio , Trovato Antonio
Published: June 2019

Proteins must fold quickly to acquire their biologically functional three-dimensional native structures. Hence, these are mainly stabilized by local contacts, while intricate topologies such as knots are rare. Here, we reveal the existence of specific patterns adopted by protein sequences and structures to deal with backbone self-entanglement. A large scale analysis of the Protein Data Bank shows that loops significantly intertwined with another chain portion are typically closed by weakly bound amino acids. Why is this energetic frustration maintained? A possible picture is that entangled loops are formed only toward the end of the folding process to avoid kinetic traps. Consistently, these loops are more frequently found to be wrapped around a portion of the chain on their N-terminal side, the one translated earlier at the ribosome. Finally, these motifs are less abundant in natural native states than in simulated protein-like structures, yet they appear in 32% of proteins, which in some cases display an amazingly complex intertwining.
Nanofiber-expanded human CD34+ cells heal cutaneous wounds in streptozotocin-induced diabetic mice
Authors: Kanji Suman , Das Manjusri , Joseph Matthew , Aggarwal Reeva , Sharma Sudarshana M. , Ostrowski Michael , Pompili Vincent J. , Mao Hai-Quan , Das Hiranmoy
Published: June 2019

Despite advances in diabetic wound care, the significant number of amputations that occur every year demands more effective therapeutics. Herein, we offer an aminated polyethersulfone nanofiber-expanded human umbilical cord blood-derived CD34+ cells (henceforth CD34+ cells) effective therapy, tested in cutaneous wounds developed in streptozotocin-induced diabetic NOD/SCID mice. We show that systemic administration of CD34+ cells homed to the wound site and significantly accelerated wound closure. Wound closure was associated with improved re-epithelialization and increased neovascularization; and with decreased sustained pro-inflammatory activity of NF-κB and its downstream effector molecules TNF-α, IL-1β, and IL-6 at the wound bed. This finding was further supported by the observation of a decreased number of myeloperoxidase positive neutrophils, and concomitantly increased levels of IL-10. In addition, improved granulation tissue formation was observed along with higher collagen deposition and myofibroblasts and decreased expressions of MMP-1. Mechanistically, CD34+ cells reduced the level of MMP-1 expression by inhibiting recruitment of NF-κB to the MMP-1 promoter site in dermal fibroblasts. In summary, we provide evidence of a novel nanofiber-expanded CD34+ stem cell therapeutic development for treating diabetic wounds by defining their cellular and molecular mechanisms.
Detection of novel biomarkers for early detection of Non-Muscle-Invasive Bladder Cancer using Competing Endogenous RNA network analysis
Authors: Kouhsar Morteza , Azimzadeh Jamalkandi Sadegh , Moeini Ali , Masoudi-Nejad Ali
Published: June 2019

Bladder Cancer (BC) is one of the most common cancers in the world. Recent studies show that non-coding RNAs such as lncRNAs and circRNAs play critical roles in the progression of this cancer, but their regulatory relationships and functions are still largely unknown. As a new regulatory process within the cell, the coding and non-coding RNAs compete with each other to sponge their target miRNAs. This mechanism is described as “the competing endogenous RNA (ceRNA) hypothesis” which provides a new perspective to understand the regulation of gene expression in health and diseases such as cancer. In this study, to investigate the role of non-coding RNAs in BC, a new approach was used to reconstruct the ceRNA network for Non-Muscle Invasive Bladder Cancer (NMIBC) based on the expression data of coding and non-coding genes. Analysis of ceRNA networks in the early stage of BC led to the detection of an important module containing the lncRNA MEG3 as the central gene. The results show that the lncRNAs CARMN, FENDRR and ADAMTS9-AS2 may regulate MEG3 in NMIBC through sponging some important miRNAs such as miR-143-3p, miR-106a-5p and miR-34a-3p. Also, the lncRNA AC007608.2 is shown to be a potential BC related lncRNA for the first time based on ceRNA stage-specific network analysis. Furthermore, hub and altered genes in stage-specific and between stage networks led to the detection of hsa_circ_0017586 and hsa_circ_0001741 as novel potential circRNAs related to NMIBC. Finally, the hub genes in the networks were shown to be valuable candidates as biomarkers for the early stage diagnosis of BC.
Synthesis of magnetic nanoflower immobilized lipase and its continuous catalytic application
Authors: Ren Weifan , Li Yao , Wang Jihui , Li Lin , Xu Longquan , Wu Yuxuan , Wang Yi , Fei Xu , Tian Jing
Published: June 2019

We have synthesized a kind of magnetic lipase–Cu3(PO4)2 nanoflowers (lipase@MNFs), which can be separated quickly from the reaction system under the external magnetic field. Compared with free lipase, the lipase@MNFs exhibited higher enzymatic activity (∼110%) and stability.
Synthesis of ion-imprinted polymer-decorated SBA-15 as a selective and efficient system for the removal and extraction of Cu(ii) with focus on optimization by response surface methodology
Authors: Ghanavati Nasab Shima , Semnani Abolfazl , Karimi Meghdad , Javaheran Yazd Mehdi , Cheshmekhezr Setareh
Published: June 2019

Ion-imprinted polymer-decorated SBA-15 (SBA-15-IIP) for the adsorption of copper was synthesized and characterized using different techniques, including FT-IR, XRD, TG/DTA, SEM, BET, and TEM.
Electrochemical sensing of cocaine in real samples based on electrodeposited biomimetic affinity ligands
Authors: Florea Anca , Cowen Todd , Piletsky Sergey , De Wael Karolien
Published: June 2019

This work assesses the fabrication of the first amperometric sensor based on electropolymerized MIPs for cocaine direct detection.
Silicon nanoparticles: fluorescent, colorimetric and gel membrane multiple detection of Cu2+ and Mn2+ as well as rapid visualization of latent fingerprints
Authors: Zhu Baoya , Tang Mingyu , Yu Liying , Qu Yaoyao , Chai Fang , Chen Lihua , Wu Hongbo
Published: June 2019

In this paper, silicon nanoparticles (Si NPs) was synthesized by a simple one-step procedure by applying uric acid as reducing agent. The Si NPs can be acted as a dual output fluorescent and colorimetric probe for selective and sensitive detection of Cu2+ and Mn2+.
Predicted M(H2)12n+ (M = Ac, Th, Pa, U, La and n = 3, 4) complexes with twenty-four hydrogen atoms bound to the metal ion
Authors: Joshi Meenakshi , Ghanty Tapan K.
Published: June 2019

The first ever report showing that a maximum of 24 hydrogen atoms can directly bind to actinide ions in M–(η2-H2)12 complexes.
Segmental Janus nanoparticles of polymer composites
Authors: Wang Ke , Li Fan , Tian Di , Xu Jiangping , Liu Yuanyuan , Hou Zaiyan , Zhou Huamin , Chen Senbin , Zhu Jintao , Yang Zhenzhong
Published: June 2019

We demonstrate a facile yet robust “plasma etching and grafting” strategy to prepare Janus nanoparticles coated with binary polymer brushes on two different sides. The ratio of two types of polymers can be tailored by tuning the plasma etching power.
Understanding and controlling the metal-directed assembly of terpyridine-functionalized coiled-coil peptides
Authors: Scheib Kimberly A. , Tavenor Nathan A. , Lawless Matthew J. , Saxena Sunil , Horne W. Seth
Published: June 2019

Spectroscopic elucidation of the interaction between terpyridine-functionalized coiled-coil peptides and Cu(ii) enables the construction of rationally designed supramolecular lattices.
Synthesis of water soluble and multi-responsive selenopolypeptides via ring-opening polymerization of N-carboxyanhydrides
Authors: Wu Guangqi , Ge Chenglong , Liu Xingyi , Wang Shuo , Wang Letian , Yin Lichen , Lu Hua
Published: June 2019

Synthesis of selenopolypeptides via ring opening polymerization of N-carboxyanhydrides.
Nickel-catalyzed borrowing hydrogen annulations: access to diversified N-heterocycles
Authors: Yang Peng , Zhang Caili , Gao Wen-Chao , Ma Yu , Wang Xiuhua , Zhang Li , Yue Jieyu , Tang Bo
Published: June 2019

An efficient nickel-catalyzed synthesis of diversified N-heterocyclesviaatom-economic borrowing hydrogen annulations of easily available alcohols and amines.
Femtosecond infrared pump–stimulated Raman probe spectroscopy: the first application of the method to studies of vibrational relaxation pathways in the liquid HDO/D2O system
Authors: Pastorczak Marcin , Nejbauer Michał , Radzewicz Czesław
Published: June 2019

We constructed a setup for fs-infrared pump–stimulated Raman probe spectroscopy and applied it to study vibrational relaxation pathways in HDO/D2O.
Amphiphilic copolymers change the nature of the ordered-to-disordered phase transition of lipid membranes from discontinuous to continuous
Authors: Zaki Afroditi Maria , Carbone Paola
Published: June 2019

The phase behaviour as a function of temperature is explored for pure phospholipid (DPPC) and hybrid lipid–polymer (DPPC/Pluronic L64) bilayers with the aid of atomistic MD simulations.
Unveiling the electronic structures and ligation effect of the superatom–polymeric zirconium oxide clusters: a computational study
Authors: Wang Jing , Zhao Yang , Li Jun , Huang Hai-Cai , Chen Jing , Cheng Shi-Bo
Published: June 2019

DFT calculations reveal the electronic structures and ligation-induced superalkali characteristics of superatom–polymeric zirconium oxide clusters.
Stabilization of AgI's polar surfaces by the aqueous environment, and its implications for ice formation
Authors: Sayer Thomas , Cox Stephen J.
Published: June 2019

AgI is a potent inorganic ice nucleating particle, a feature often attributed to the lattice match between its {0001} surfaces and ice. Dissolved ions are found to be essential to the stability of these polar surfaces, and crucial to ice formation.
Assimilation and transport of organic bound tritium in an irrigated pine forest
Authors: Duff Martine C. , Kuhne Wendy W. , Viner Brian , Swindle Ashlee , Houk Amanda L. , Chiswell Steven , Hunter Douglas B. , Rivera Omar E.
Published: June 2019

After 2 decades of irrigation with groundwater from a holding pond: OBT and 14C are retained in the recent wood growth whereas HTO levels remained constant with wood age.
Water-induced self-assembly of mixed gelator system (ceramide and lecithin) for edible oil structuring
Authors: Guo Shenglan , Song Mingyue , He Xiaoting , Yang Fuyu , Cao Yong , Rogers Michael , Lan Yaqi
Published: June 2019

In the presence of a small amount of water, CER and LEC were self-assembled to form oleogels in sunflower oil. This can be used to produce hard-stock fat replacers as well as for incorporating water-soluble nutrients into oil-based products.
Triple copper catalysis for the synthesis of vinyl triazoles
Authors: Baldassari Lucas L. , Cechinatto Eduardo A. , Moro Angélica V.
Published: June 2019

Herein, we report our studies on the synthesis of vinyl 1,2,3-triazoles through a one-pot sequence, enabled by the same copper catalyst, of three different reactions: hydroboration involving an alkyne, azidation for a vinyl boronate and azide–alkyne cycloaddition.
Fabrication and characterization of biocompatible pH responsive halloysite nanotubes grafted with sodium alginate for sustained release of phenytoin sodium
Authors: Zeraatpishe Leila , Mohebali Alireza , Abdouss Majid
Published: June 2019

Schematic design of a new method for fabrication of biocompatible pH sensitive halloysite nanocomposites for controlled release of phenytoin sodium.
Redox-switching of ternary Ni(ii) and Cu(ii) complexes: synthesis, experimental and theoretical studies along with second-order nonlinear optical properties
Authors: Novoa Néstor , Manzur Carolina , Roisnel Thierry , Dorcet Vincent , Cabon Nolwenn , Robin-Le Guen Françoise , Ledoux-Rak Isabelle , Kahlal Samia , Saillard Jean-Yves , Carrillo David , Hamon Jean-René
Published: June 2019

The redox-switching behavior of the title compounds has been probed for the Ni(ii) derivatives using time-resolved spectroelectrochemistry under thin-layer conditions.
The effect of different metal oxides on the catalytic activity of a Co3O4catalyst for toluene combustion: importance of the structure–property relationship and surface active species
Authors: Zhang Xuejun , Zhao Min , Song Zhongxian , Zhao Heng , Liu Wei , Zhao Jinggang , Ma Zi'ang , Xing Yun
Published: June 2019

The Co–La catalyst was used to the catalytic oxidation of toluene. The LaCoO3perovskite was detected. The abundance of Co3+and active oxygen contributed synergistically to the redox cycle reaction, which could improve the catalytic activity.
Effect of secondary interactions, steric hindrance and electric charge on the interaction of VIVO species with proteins
Authors: Sciortino Giuseppe , Sanna Daniele , Ugone Valeria , Maréchal Jean-Didier , Alemany-Chavarria Mercè , Garribba Eugenio
Published: June 2019

The effect of secondary interactions (hydrogen bonds and van der Waals contacts), steric hindrance and electric charge, on the binding of VIVcomplexes formed by pipemidic and 8-hydroxyquinoline-5-sulphonic acids with ubiquitin and lysozyme is studied.
Construction of a hollow porous carbon spheres@CoP/nitrogen-doped carbon supported platinum catalyst for high performance methanol oxidation
Authors: Zhang Huanhuan , Ma Li , Gan Mengyu , Xie Fei , He Hongmei , Hu Liangqing , Jiang Minghang
Published: June 2019

A Pt–HPCS@CoP/NC catalyst with excellent catalytic activity, CO-tolerance and durability was synthesized for the methanol oxidation reaction.
Erythrocyte-derived vesicles for circulating tumor cell capture and specific tumor imaging
Authors: Chen Ming , Liu Ao , Chen Bei , Zhu Dao-Ming , Xie Wei , Deng Fang-Fang , Ji Li-Wei , Chen Li-Ben , Huang Hui-Ming , Fu You-Rong , Liu Wei , Wang Fu-Bing
Published: June 2019

The precise diagnosis of cancer remains a great challenge; therefore, it is our research interest to develop safe, tumor-specific reagents.
2D ultrathin carbon nanosheets with rich N/O content constructed by stripping bulk chitin for high-performance sodium ion batteries
Authors: Gao Lingfeng , Ma Jingqi , Li Shuping , Liu Dajin , Xu Dingfeng , Cai Jie , Chen Lingyun , Xie Jia , Zhang Lina
Published: June 2019

The layered structure assisted efficient production of 2D carbon nanosheets from natural chitin is reported, demonstrating excellent performance in sodium storage.
Ionic-liquid-bifunctional wrapping of ultrafine SnO2 nanocrystals into N-doped graphene networks: high pseudocapacitive sodium storage and high-performance sodium-ion full cells
Authors: Yang Yan , Pan Zhen-Zhen , Wang Ying-Ying , Ma Yuan-Chuan , Li Chong , Lu Yu-Jun , Wu Xing-Long
Published: June 2019

N-Doped graphene wrapped ultrafine SnO2 nanocrystals were designed and prepared by a simple ionic liquid assisted hydrothermal method.
Thermally enhanced NIR–NIR anti-Stokes emission in rare earth doped nanocrystals
Authors: Mi Chao , Zhou Jiajia , Wang Fan , Jin Dayong
Published: June 2019

Surface phonons enhancing the one-photon anti-Stokes fluorescence has been demonstrated in rare earth doped nanocrystals.
WO3 nanosheets rich in oxygen vacancies for enhanced electrocatalytic N2 reduction to NH3
Authors: Kong Wenhan , Zhang Rong , Zhang Xiaoxue , Ji Lei , Yu Guangsen , Wang Ting , Luo Yonglan , Shi Xifeng , Xu Yuanhong , Sun Xuping
Published: June 2019

WO3 nanosheets rich in oxygen vacancies are efficient for electrocatalytic N2-to-NH3 fixation under ambient conditions with a NH3 yield of 17.28 μg h−1 mgcat.−1 and a faradaic efficiency of 7.0% in 0.1 M HCl.
High photoluminescence of shortwave infrared-emitting anisotropic surface charged gold nanoclusters
Authors: Musnier Benjamin , Wegner K. David , Comby-Zerbino Clothilde , Trouillet Vanessa , Jourdan Muriel , Häusler Ines , Antoine Rodolphe , Coll Jean-Luc , Resch-Genger Ute , Le Guével Xavier
Published: June 2019

Incorporating anisotropic surface charges on atomically precise gold nanoclusters led to a intense shortwave infrared photoluminescence exceeding 1100 nm with QY up to 6.1%.
Facile construction of diverse polyheterocyclic scaffolds via gold-catalysed dearomative spirocyclization/1,6-addition cascade
Authors: He Yi , Wu Danjun , Li Zhenghua , Robeyns Koen , Van Meervelt Luc , Van der Eycken Erik V.
Published: June 2019

A post-Ugi gold-catalysed chemo- and diastereo-selective cascade dearomative spirocyclization/1,6-addition sequence is disclosed for the facile synthesis of diverse fused polyheterocyclic scaffolds bearing indole, pyrrole, benzothiophene, furan or electron-rich arene cores.
Investigation of plasma metabolomics and neurotransmitter dysfunction in the process of Alzheimer's disease rat induced by amyloid beta 25-35
Authors: Wei Mengying , Liu Yuanyuan , Pi Zifeng , Yue Kexin , Li Shizhe , Hu Mingxin , Liu Zhiqiang , Song Fengrui , Liu Zhongying
Published: June 2019

The cognitive impairment, Alzheimer's disease (AD) related enzymes, neurotransmitters and endogenous metabolites shown a dynamic change in AD model rat induced by amyloid beta 25-35.
Fe3+-codoped ultra-small NaGdF4:Nd3+ nanophosphors: enhanced near-infrared luminescence, reduced particle size and bioimaging applications
Authors: Li Yabing , Li Fujin , Huang Yanan , Wu Haiyan , Wang Jian , Yang Jin , Xiao Qingbo , Lin Hongzhen
Published: June 2019

Small-sized lanthanide-doped nanoparticles (NPs) exhibiting superior near-infrared (NIR) luminescence properties are highly desired for bioimaging applications.
The effect of fermentation conditions on the structure and anti-tumor activity of polysaccharides from Cordyceps gunnii
Authors: Liu Xiao-Cui , Li Hongran , Kang Tong , Zhu Zhen-Yuan , Liu Ying-Liang , Sun Hui-Qing , Pan Li-chao
Published: June 2019

This study investigates the effect of fermentation conditions on the structure and anti-tumor activity of intracellular polysaccharides (IPS) of Cordyceps gunnii (C. gunnii) in submerged fermentation.
Photoactive cotton fabric for UV protection and self-cleaning
Authors: Ahmad Ishaq , Kan Chi-wai , Yao Zhongping
Published: June 2019

Development of textile materials with tailored properties by coating with nano-materials is an emerging field of research.
Noninvasive diagnostic methods for diabetes mellitus from tear fluid
Authors: Glinská Gabriela , Krajčíková Kristína , Zakutanská Katarína , Shylenko Oleg , Kondrakhova Daria , Tomašovičová Natália , Komanický Vladimír , Mašlanková Jana , Tomečková Vladimíra
Published: June 2019

In our pilot study we assessed the diagnostic potential of FS, IRS and AFM for diabetes mellitus using tear fluid.
Mimicking growth factors: role of small molecule scaffold additives in promoting tissue regeneration and repair
Authors: Goonoo Nowsheen , Bhaw-Luximon Archana
Published: June 2019

Scaffold loaded with small molecules mimicking the action of growth factors for tissue repair.
Microfabricated needle for hydrogen peroxide detection
Authors: Feng Shilun , Clement Sandhya , Zhu Yonggang , Goldys Ewa M. , Inglis David W.
Published: June 2019

A droplet-based microfluidic needle-like probe has been designed and applied for on-site hydrogen peroxide (H2O2) sampling, reaction and detection using a commercial, single-step fluorescent H2O2 assay.
Sizable bandgaps of graphene in 3d transition metal intercalated defective graphene/WSe2 heterostructures
Authors: Zhang Xiuyun , Sun Yi , Gao Weicheng , Lin Yin , Zhao Xinli , Wang Qiang , Yao Xiaojing , He Maoshuai , Ye Xiaoshan , Liu Yongjun
Published: June 2019

3d transition metal (TM = Sc–Ni) atom-intercalated G/WSe2 heterostructures, as well as their defective configurations having single vacancies on graphene or WSe2 sublayers, are studied.
Determination of the absolute configuration of conformationally flexible molecules by simulation of chiro-optical spectra: a case study
Authors: Mancinelli Michele , Franzini Roberta , Renzetti Andrea , Marotta Emanuela , Villani Claudio , Mazzanti Andrea
Published: June 2019

The absolute configuration (AC) assignment of two very flexible organic molecules by TD-DFT simulation of ECD spectra is evaluated by modifying the calculation level in the various steps of the AC assignment process.
Rh(iii)-catalyzed C-7 arylation of indolines with arylsilanes via C–H activation
Authors: Luo Haiqing , Xie Qi , Sun Kai , Deng Jianbo , Xu Lin , Wang Kejun , Luo Xuzhong
Published: June 2019

Site-selective synthesis of C-7 arylated indolines has been achieved via oxidative arylation of indolines with arylsilanes under Rh(iii)-catalyzed C–H activation of indolines.
Interplay of secondary and tertiary folding in abiotic foldamers
Authors: Mazzier Daniela , De Soumen , Wicher Barbara , Maurizot Victor , Huc Ivan
Published: June 2019

Helical secondary folds are stabilized within abiotic helix–turn–helix tertiary structures in organic solvents.
Thermodynamic and kinetic studies of H2 and N2 binding to bimetallic nickel-group 13 complexes and neutron structure of a Ni(η2-H2) adduct
Authors: Cammarota Ryan C. , Xie Jing , Burgess Samantha A. , Vollmer Matthew V. , Vogiatzis Konstantinos D. , Ye Jingyun , Linehan John C. , Appel Aaron M. , Hoffmann Christina , Wang Xiaoping , Young Victor G. , Lu Connie C.
Published: June 2019

Binding energies for H2 and N2 at nickel become more exergonic for the larger group 13 sigma-accepting supports.
Storing redox equivalent in the phenalenyl backbone towards catalytic multi-electron reduction
Authors: Bhunia Mrinal , Sahoo Sumeet Ranjan , Shaw Bikash Kumar , Vaidya Shefali , Pariyar Anand , Vijaykumar Gonela , Adhikari Debashis , Mandal Swadhin K.
Published: June 2019

Redox equivalent storage in the phenalenyl backbone towards catalytic multi-electron reduction.
Facilitation of DNA loop formation by protein–DNA non-specific interactions
Authors: Shin Jaeoh , Kolomeisky Anatoly B.
Published: June 2019

DNA looping is facilitated by non-specific protein–DNA interactions.
Schiff base-bridged TPE-rhodamine dyad: facile synthesis, distinct response to shearing and hydrostatic pressure, and sequential multicolored acidichromism
Authors: Liu Xuedan , Ma Chunran , Li Aisen , Xu Weiqing , Ma Zhiyong , Jia Xinru
Published: June 2019

A newly designed and facilely synthesized Schiff base-bridged TPE-rhodamine dyad that achieves AIEE, distinct response to shearing and hydrostatic pressure and intriguing sequential multicolored acidichromism, owing to the imine group and spiro-lactam structure.
Spin polarization and magnetic properties at the C60/Fe4N(001) spinterface
Authors: Han Xuefei , Mi Wenbo , Wang Xiaocha
Published: June 2019

C60-Induced redistribution of Fe d-orbitals results in magnetic moment inversion, and the inverted spatial spin polarization is obtained.
A Comparison of Three Discourse Elicitation Methods in Aphasia and Age-Matched Adults: Implications for Language Assessment and Outcome
Authors: Stark Brielle C.
Published: June 2019

Purpose

Discourse analysis is commonly used to assess language ability and to evaluate language change following intervention in aphasia. The purpose of this study was to identify differences in language produced during different discourse tasks in a large aphasia group and an age- and education-matched control group.

Method

Four structured discourse tasks across 3 discourse types (expositional, narrative, and procedural) were evaluated in a group of adults with aphasia (
n
= 90) and an age-matched control group (
n
= 84) drawn from AphasiaBank. CLAN software was used to extract primary linguistic variables (mean length of utterance, propositional density, type–token ratio, words per minute, open–closed class word ratio, noun–verb ratio, and tokens), which served as proxies for various language abilities. Using a series of repeated-measures analyses of covariance, with significantly correlated demographic and descriptive variables as covariates, main effects of discourse type were evaluated.

Results

Despite an impoverished output from the aphasia group (i.e., the control group produced significantly more overall output), there was a main effect of discourse type on most primary linguistic variables in both groups, suggesting that, in adults with and without language impairments, each discourse type taxed components of the spoken language system to varying extents. Post hoc tests fleshed out these results, demonstrating that, for example, narrative discourse produced speech highest in propositional density.

Conclusion

Each discourse type taxes the language system in different ways, verifying the importance of using several discourse tasks and selecting the most sensitive discourse tasks when evaluating specific language abilities and outcomes.
Volubility, Consonant Emergence, and Syllabic Structure in Infants and Toddlers Later Diagnosed With Childhood Apraxia of Speech, Speech Sound Disorder, and Typical Development: A Retrospective Video Analysis
Authors: Overby Megan S. , Caspari Susan S. , Schreiber James
Published: June 2019

Purpose

Studies of infants' early vocalizations have proven helpful in describing the developmental characteristics of various communication disorders. However, few studies have addressed the early vocalizations of infants and toddlers who were later diagnosed, as older children, with childhood apraxia of speech (CAS). We refer to these infants and toddlers as
LCAS
. Extant studies also often lack a comparison group of infants and toddlers who were later diagnosed, as older children, with a speech sound disorder (SSD). We refer to these infants and toddlers as
LSSD
. We aimed to compare the volubility, consonant emergence, and syllabic structure from birth to age of 2 years, as observed in home videos, among 3 groups of infants and toddlers: LCAS, LSSD, and typically developing (TD).

Method

We assessed the speech-language skills of 17 children (3.5–8.8 years old; 7 with CAS, 5 with SSD, and 5 TD) and transcribed home videos (obtained from parents) of these same children from birth to age of 2 years. Early vocalizations were coded as nonresonant or resonant. Nonresonant vocalizations could not be transcribed with the International Phonetic Alphabet. Resonant (speechlike) vocalizations were broadly transcribed, and resonant consonants were categorized by place, manner, and voicing.

Results

Effect size comparisons revealed LCAS infants and toddlers were less voluble, used fewer resonant consonants, had a less diverse phonetic repertoire, and acquired resonant consonants later than either the LSSD or TD participants. For LSSD infants and toddlers, means for these dependent variables were lower than the means demonstrated by the TD group, but effect size were not strong due to LSSD variability.

Conclusions

Findings imply there might be clinical “red flags” that could assist the identification of infants and toddlers at risk for later diagnosis of CAS. Data did not support red flags for identifying infants and toddlers at risk for later diagnosis of SSD. Because of significant study limitations, results obtained should be considered preliminary.

Supplemental Material

https://doi.org/10.23641/asha.8233334
Optically variable AGN in the three-year VST survey of the COSMOS field
Authors: De Cicco D. , Paolillo M. , Falocco S. , Poulain M. , Brandt W. N. , Bauer F. E. , Vagnetti F. , Longo G. , Grado A. , Ragosta F. , Botticella M. T. , Pignata G. , Vaccari M. , Radovich M. , Salvato M. , Covone G. , Napolitano N. R. , Marchetti L. , Schipani P.
Published: June 2019

Context. The analysis of the variability of active galactic nuclei (AGN) at different wavelengths and the study of possible correlations of different spectral windows are a current main field of inquiry. Optical variability has been largely used to identify AGN in multivisit surveys. The strength of a selection based on optical variability lies in the opportunity of analyzing data from surveys of large sky areas by ground-based telescopes. However, the effectiveness of optical variability selection with respect to other multiwavelength techniques has been poorly studied down to the depth that is expected from next-generation surveys.

Aims. Here we present the results of our r-band analysis of a sample of 299 optically variable AGN candidates in the VST survey of the COSMOS field, counting 54 visits spread over three observing seasons spanning more than three years. This dataset is more than three times larger than the dataset presented in our previous analysis, and the observing baseline is about eight times longer.

Methods. We push toward deeper magnitudes (r(AB) ∼ 23.5 mag) than were reached in past studies. We made wide use of ancillary multiwavelength catalogs in order to confirm the nature of our AGN candidates, and constrained the accuracy of the method based on spectroscopic and photometric diagnostics. We also performed tests aimed at assessing the relevance of dense sampling in view of future wide-field surveys.

Results. We demonstrate that the method allows the selection of high-purity (> 86%) samples. We take advantage of the longer observing baseline to achieve great improvement in the completeness of our sample with respect to X-ray and spectroscopically confirmed samples of AGN (59% vs. ∼15% in our previous work), as well as in the completeness of unobscured and obscured AGN. The effectiveness of the method confirms the importance of developing future more refined techniques for the automated analysis of larger datasets.
Study of various organophosphonate additives as calcium carbonate inhibitors for reverse osmosis
Authors: Andrianov Alexey , Govorova Zhanna
Published: June 2019

Various chemical additives are used in water treatment industry, among them – precipitation inhibitors for reverse osmosis membrane facilities. The development and synthesis of new inhibitor formulas and the study of their effectiveness is still an urgent task for many researchers working in this field. The relationship between the structure of phosphonate scale inhibitors and their effectiveness in preventing of calcium carbonate precipitation was studied. Two classes of tetraphosphonate and diphosphonate inhibitors that possess systematic structural similarities and differences have been tested on the laboratory membrane unit. All chemicals were tested at doses of 1 and 5 mg/L. The results showed that the antiscalant efficiency for both groups of inhibitors increases with elongation of methylene chain, but the longest compounds have a sharp drop in inhibition efficiency.
The use of solar radiation for generating heat in a solar air collector in northern Poland
Authors: Fieducik Jolanta
Published: June 2019

This article analyzes the use of solar radiation for generating heat in a simple solar air collector built in a laboratory. The structure of the collector and the equipment for measuring physical parameters were described. The aim of the study was to analyze the operating parameters and the thermal efficiency of a solar air collector in northern Poland. Various applications of solar air collectors were discussed. Solar air collectors can be used for heating, ventilating and drying indoor premises and for heating water.