bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Different Histone Deacetylase Inhibitors in Attenuating Inflammatory Pain in Rats
Authors: Mao Yu , Zhou Jing , Liu Xuesheng , Gu Erwei , Zhang Zhi , Tao Wenjuan
Published: January 2019

Histone deacetylase inhibitors (HDACIs), which interfere with the epigenetic process of histone acetylation, have shown analgesic effects in animal models of persistent pain. The HDAC family comprises 18 genes; however, the different effects of distinct classes of HDACIs on pain relief remain unclear. The aim of this study was to determine the efficacy of these HDACIs on attenuating thermal hyperalgesia in persistent inflammatory pain. Persistent inflammatory pain was induced by injecting Complete Freund’s Adjuvant (CFA) into the left hind paw of rats. Then, HDACIs targeting class I (entinostat (MS-275)) and class IIa (sodium butyrate, valproic acid (VPA), and 4-phenylbutyric acid (4-PBA)), or class II (suberoylanilide hydoxamic acid (SAHA), trichostatin A (TSA), and dacinostat (LAQ824)) were administered intraperitoneally once daily for 3 or 4 days. We found that the injection of SAHA once a day for 3 days significantly attenuated CFA-induced thermal hyperalgesia from day 4 and lasted 7 days. In comparison with SAHA, suppression of hyperalgesia by 4-PBA peaked on day 2, whereas that by MS-275 occurred on days 5 and 6. Fatigue was a serious side effect seen with MS-275. These findings will be beneficial for optimizing the selection of specific HDACIs in medical fields such as pain medicine and neuropsychiatry.
Frequent Antenatal Care Visits Increase Institutional Delivery at Dabat Health and Demographic Surveillance System Site, Northwest Ethiopia
Authors: Fekadu Abel , Yitayal Mezgebu , Alemayehu Geta Asrade , Abebe Solomon Mekonnen , Ayele Tadesse Awoke , Tariku Amare , Andargie Gashaw , Teshome Destaw Fetene , Gelaye Kassahun Alemu
Published: January 2019

Background. Early diagnosis of pregnancy, professional follow-up, and skilled delivery service are the main interventions that reduce maternal morbidity and mortality. Generating local based evidence could support targeted and effective intervention placed by a government. Therefore, determining the prevalence of skilled institutional delivery and its associated factors is of supreme importance. Methods. A community based cross-sectional study was conducted among pregnant women at Dabat Health and Demographic Surveillance System (DHDSS) site from 2014 to 2015. A total of 1290 pregnant women were included in the study. Data were extracted from what was collected as part of the ongoing DHDSS. Variables were extracted from the Household Registration System (HRS2 version 2.1) database and exported to STATA version 14.1 for analysis. Binary logistic regression was used to identify the factors associated with skilled institutional delivery. Statistical test was considered significant at P value < 0.05. Results. The proportion of skilled institutional delivery was 31.0% (95% CI: 28.5, 33.6). Frequent Antenatal care (ANC) visits (Adjusted Odds Ratio (AOR): 2.94; 95% CI: 1.75, 4.94)), living in urban setting (AOR: 9.54; 95% CI: 5.99, 15.17), and ability to read and write (AOR: 1.81; 95% CI: 1.18, 2.75) were factors associated with increased delivery in the health institutions. On the other hand, giving more number of births (AOR: 0.39; 95% CI: 0.22, 0.66) decreased health institution delivery by 61%. Conclusion. Higher rate of skilled institutional delivery has been observed at the surveillance site as compared with the previous national estimates. Giving less number of births, frequent ANC visits, being in urban residence, and ability to read and write increased the likelihood of health institution delivery. Strengthening interventions that could influence the identified factors could improve mothers’ choice to skilled institutional delivery.
Antipathogenic Potential of a Polyherbal Wound-Care Formulation (Herboheal) against Certain Wound-Infective Gram-Negative Bacteria
Authors: Patel Pooja , Joshi Chinmayi , Kothari Vijay
Published: January 2019

This study investigated antipathogenic efficacy of a polyherbal wound-healing formulation Herboheal against three multidrug-resistant strains of gram-negative bacterial pathogens associated with wound infections. Herboheal was evaluated for its quorum-modulatory potential against three different human-pathogenic bacteria, firstin vitrothrough the broth dilution assay and thenin vivoin the model hostCaenorhabditis elegans. Herboheal at ≥0.1% v/v was able to inhibit (19–55%)in vitroproduction of quorum sensing-regulated pigments in all these bacteria and seemed to interfere with bacterial quorum sensing by acting as a signal-response inhibitor. This formulation could compromise haemolytic activity of all three bacteria by ∼18–69% and induced their catalase activity by ∼8–21%. Herboheal inhibitedP. aeruginosabiofilm formation up to 40%, reduced surface hydrophobicity ofP. aeruginosacells by ∼9%, and also made them (25%) more susceptible to lysis by human serum. Antibiotic susceptibility of all three bacteria was modulated owing to pretreatment with Herboheal. Exposure of these test pathogens to Herboheal (≥0.025% v/v) effectively reduced their virulence towards the nematodeCaenorhabditis elegans. Repeated subculturing ofP. aeruginosaon the Herboheal-supplemented growth medium did not induce resistance to Herboheal in this mischievous pathogen, and this polyherbal extract was also found to exert a post-extract effect onP. aeruginosa, wherein virulence of the Herboheal-unexposed daughter cultures, of the Herboheal-exposed parent culture, was also found to be attenuated. Overall, this study indicates Herboheal formulation to be an effective antipathogenic preparation and validates its indicated traditional therapeutic use as a wound-care formulation.
Levels of CEACAM6 in Peripheral Blood Are Elevated in Patients with Plasma Cell Disorders: A Potential New Diagnostic Marker and a New Therapeutic Target?
Authors: Steiner N. , Hajek R. , Nachbaur D. , Borjan B. , Sevcikova S. , Göbel G. , Gunsilius E.
Published: January 2019

Introduction. The prognosis of multiple myeloma is still unfavorable due to inherent characteristics of the disease and the often-delayed diagnosis due to widespread and unspecific symptoms such as back pain and fatigue. Therefore, a simple diagnostic blood test would be helpful to speed up the diagnostic procedure in such patients (pts.). Here, we evaluated the diagnostic value of plasma levels of carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) in the peripheral blood and bone marrow of pts. with plasma cell disorders and in healthy controls. Materials and Methods. Immunoreactive CEACAM6 was determined in the peripheral blood and bone marrow (n=95/100) of pts. with monoclonal gammopathy of unknown significance (MGUS: 28/37), newly diagnosed multiple myeloma (NDMM: 42/40), and relapsed/refractory multiple myeloma (RRMM: 25/23) by sandwich ELISA. Results. Median CEACAM6 levels in the peripheral blood of pts. with plasma cell disorders were significantly higher than those of healthy controls (healthy controls: 15.2 pg/ml (12.1-17.1); MGUS: 19.0 pg/ml (16.4-22.5); NDMM: 18.0 pg/ml (13.4-21.2); and RRMM: 18.9 pg/ml (15.2-21.5); p<0.001). Plasma levels of CEACAM6 discriminated healthy subjects from MGUS/NDMM pts. (AUC=0.71, 95% CI: 0.6-0.8); i.e., a CEACAM6 level>17.3 pg/ml has an 82% (95% CI: 70-90) predictive probability for the identification of MGUS or NDMM. Moreover, CEACAM6 levels in the bone marrow were significantly higher in RRMM pts. than in NDMM pts. (p=0.04), suggesting a role of this molecule in disease progression. Conclusion. CEACAM6 plasma levels can noninvasively identify pts. with a plasma cell disorder and should be evaluated prospectively as a potential diagnostic marker. Moreover, due to high CEACAM6 levels in the bone marrow in RRMM pts., this adhesion molecule might be a therapeutic target in multiple myeloma pts.
Evaluation of the Treatment Effect of Aloe vera Fermentation in Burn Injury Healing Using a Rat Model
Authors: Hai Zhiwen , Ren Yimeng , Hu Jiawen , Wang Huan , Qin Qi , Chen Tingtao
Published: January 2019

Burn injury is a growing medical problem associated with public health, and few effective agents are available for treatment of this disease. In the present study, a burn injury rat model was developed and the accelerated effect of Aloe vera fermentation on burn injury healing was evaluated. Our results indicated that Aloe vera fermentation could markedly reduce the DPPH (56.12%), O2⋅− (93.5%), ⋅OH (76.12%), Fe2+ chelation (82%), and oxygen-reduction activity (0.28 μg/ml) and significantly inhibited the growth of pathogens S. typhimurium ATCC 13311 (inhibition zone diameter: 14 mm), S. enteritidis ATCC13076 (IZD: 13 mm), S. flexneri ATCC 12022 (IZD: 18 mm), E. coli 44102 (IZD: 10 mm), L. monocytogenes ATCC 19111 (IZD: 18 mm), S. dysenteriae 301 (IZD: 20 mm), S. aureus COWAN1 (IZD: 19 mm), and P. acnes ATCC 11827 (IZD: 25 mm) in vitro. The in vivo results indicated that Aloe vera fermentation produced more eosinophils and fibroblasts and less vessel proliferation compared with the model group on the 14th day, which had greatly accelerated burn injury healing via shedding of the scab and promoting hair growth. ELISA results indicated that Aloe vera fermentation had significantly reduced the production of proinflammatory factors TNF-α and IL-1β (p<0.05) and greatly enhanced the yield of anti-inflammatory factor IL-4 in animal serum (p<0.05). In addition, the high-throughput sequencing results indicated that Aloe vera fermentation obviously increased the percentage of Firmicutes (65.86% vs. 49.76%), while reducing the number of Bacteroidetes (27.60% vs. 45.15%) compared with the M group at the phylum level. At the genus level, Aloe vera fermentation increased the probiotic bacteria Lactobacillus (3.13% vs. 2.09%) and reduced the pathogens Prevotella (10.60% vs.18.24%) and Blautia (2.91% vs. 16.41%) compared with the M group. Therefore, we concluded that the use of Aloe vera fermentation significantly accelerates burn injury healing via reduction of the severity of inflammation and through modification of gut microbiota.
Upper Bound Analysis for Collapse Failure of Shield Tunnel Face Excavated in Unsaturated Soils Considering Steady Vertical Flow
Authors: Zhang Jia-hua , Wang Wei-jun , Zhang Biao , Zhang Dao-bing
Published: January 2019

Natural soils are mostly in an unsaturated state and the corresponding mechanical properties differ significantly from a saturated one. In traditional stability analysis for a shield tunnel face, the soil mass is typically assumed to be dry or saturated for convenience of analysis. In this work, based on the upper bound theorem of classical plasticity theory and the log‐spiral failure mechanism, face stability of the shield tunnel excavated in unsaturated soils under vertical steady flow is studied. The profile of shear strength is determined by virtues of the unified effective stress approach and the analytical solution of matric suction under unsaturated steady flow. On this basis, the analytical expression for the supporting pressure is deduced and the sequential quadratic programming is employed to search for the optimal upper bound solution. Through parametric analysis it is found the fitting parameters of SWCC and the groundwater level affect the supporting pressure notably. Besides, the relevant influence of vertical discharge is also significant when clayey soils are concerned.
Effect of a Novel Patient Garment on Perceived Privacy during Colonoscopy: A Simple Approach to Minimize Embarrassment
Authors: Aamar Ali , Butt Zeeshan , Madhani Kamraan , Hussain Iqra , Garsten Joel , Aslanian Harry
Published: January 2019

Background. In the United States, patients wear a one-piece, reusable cloth gown during colonoscopy procedures. Many patients report embarrassment related to bodily exposure during colonoscopy. This may limit participation in colorectal cancer screening programs. Aims. To assess whether the use of a novel, disposable patient garment (Privacy Pants, Jackson, MS), which increases patient coverage, can reduce embarrassment related to bodily exposure and increase colonoscopy acceptance rates. Methods. Patients were offered a novel gown, and they completed questionnaires before and after colonoscopy. Results. A total of 120 patients participated. 54% were female and 82% were Caucasian. The novel gown had high overall satisfaction (8.3) and was associated with a sense of respect during the procedure (9.4). 67% (80) of the patients had a prior colonoscopy, and of these, 76% would request a novel gown over a traditional gown for future procedures. Among all study participants, a high rate of acceptability for repeat colonoscopy if recommended by their doctors was reported (mean of 9.4). Nonwhites were more likely to have a concern for embarrassment addressed by using novel gowns as compared to whites (P value 0.02). Conclusion. All participants, particularly women and nonwhite participants, reported high rates of respect and satisfaction and decrease in embarrassment utilizing the novel gown during colonoscopy. Patients who had prior colonoscopy with a traditional gown preferred the novel garment. A novel procedure gown may enhance colonoscopy acceptance by minimizing embarrassment.
COL11A1 Polymorphisms Are Associated with Primary Angle-Closure Glaucoma Severity
Authors: Wan Yani , Li Shengjie , Gao Yanting , Tang Li , Cao Wenjun , Sun Xinghuai
Published: January 2019

Purpose. PLEKHA7 and COL11A1 were genotyped for single-nucleotide polymorphisms (SNPs) to investigate the possible association of these two genes with primary angle-closure glaucoma (PACG) and disease severity. Method. A total of 51 PACG cases and 51 normal controls were recruited. Twelve SNPs in the PLEKHA7 (rs216489, rs1027617, rs366590, rs11024060, rs6486330, rs11024097, and rs11024102) and COL11A1 (rs1676484, rs3753841, rs12138977, rs2126642, and rs2622848) genes were genotyped by direct Sanger sequencing. Distributions of allele frequencies and genotype frequencies in cases and controls, as well as in mild, moderate, and severe subgroups, were compared based on mean defect (MD ≤ 6.00 dB, 6 dB < MD ≤ 12 dB, and MD > 12 dB were considered mild, moderate, and severe, respectively). Independent Student’s t-tests and chi-square tests were used to compare characteristics of PACG cases and controls. Chi-square tests were used to compare the distribution of allele frequencies in cases and controls and in MD-based subgroups with various degrees of glaucoma severity. Binary logistic regression was used to compare the distribution of genotype frequencies and calculate odds ratios (OR) with confidence intervals (CI). Result. Three of the 12 SNPs in COL11A1, rs1676486 (P=0.026, OR = 2.089, 95% CI = 1.092–3.996), rs3753841 (P=0.036, OR = 1.886, 95% CI = 1.038–3.426), and rs12138977 (P=0.024, OR = 2.133, 95% CI = 1.104–4.123) were found to have a significant association with PACG. Furthermore, in the subgroup analysis, rs1676486 (P=0.018, OR = 2.416, 95% CI = 1.284–4.544; P=0.011, OR = 2.119, 95% CI = 1.204–3.729), rs12138977 (P=0.009, OR = 2.158, 95% CI = 1.287–3.618; P=0.006, OR = 1.962, 95% CI = 1.239–3.106), and rs3753841 (P=0.007, OR = 2.550, 95% CI = 1.344–4.839) showed statistically significant differences between moderate/severe groups and controls. Conclusion. Our data suggested that COL11A1 rs1676484, rs3753841, and rs12138977 polymorphisms may be of value for further study as potential gene-dependent risk factors for developing PACG. Moreover, COL11A1 rs1676484 and rs12138977 polymorphisms might be associated with PACG disease severity.
Research on HMCVT Efficiency Model Based on the Improved SA Algorithm
Authors: Cheng Zhun , Lu Zhixiong , Dai Fang
Published: January 2019

Research on the efficiency characteristic of the hydromechanical continuous variable transmission (HMCVT) in tractors is key to obtaining optimal transmission, developing control strategies, and assessing efficiency. To ease and improve the accuracy of obtaining the efficiency completely based on test measurements or theoretical calculation, this study proposes a method for building the HMCVT efficiency model. The method is based on an improved simulated annealing (SA) algorithm according to a small amount of test data. The study uses 8 groups of transmission efficiency values under different operating conditions obtained from bench tests. By theoretical analysis of the HMCVT, this study divides the total transmission efficiency into (i) the transmission efficiency from the output power of the power source to the confluence mechanism, (ii) the transmission efficiency of the confluence mechanism, and (iii) the transmission efficiency of the output part after confluence. The formulas for the three parts of transmission efficiencies are then derived. This study improves the SA algorithm and uses it to identify the three key parameters of hydraulic systems of the transmission efficiency calculation model. Research results indicate that the efficiency model built using the proposed method exhibits high accuracy with an error of about 1.90%. The improved SA algorithm can rapidly complete key parameter identification with an error of about 2.16%; when the displacement ratio is 0, the efficiency values at the same stage are approximately equal under different operating conditions. The HMCVT efficiency model can be built rapidly and effectively with only five groups of efficiency measurement tests.
Euglycemic Ketoacidosis in Spinal Muscular Atrophy
Authors: Stoimenis Dimitrios , Spyridonidou Christina , Theofanidou Sofia , Petridis Nikolaos , Papaioannou Nikos , Iasonidou Christina , Kapravelos Nikolaos
Published: January 2019

Euglycemic ketoacidosis is defined by the triad of high anion gap acidosis, increased plasma ketones, and the absence of hyperglycemia. Apart from diabetes mellitus, the disorder may occur in prolonged fasting, excessive alcohol consumption, pregnancy, and inborn errors of metabolism. Here, we highlight the diagnosis of euglycemic ketoacidosis in a pediatric nondiabetic patient with spinal muscular atrophy (SMA) type 1 (Werdnig–Hoffmann disease), who, subsequently to her postoperative admission to the intensive care unit following a spinal surgery, developed high anion gap metabolic acidosis. We discuss the pathophysiology of acid-base disorders in SMA, along with the glucose and fatty acids metabolism, the necessary knowledge for medical practitioners.
Vitamin D Regulates the Expressions of AQP-1 and AQP-4 in Mice Kidneys
Authors: Fu Yu , Zhu Jiajun , Zhang Yalin , Liu Zuwang , Su Han , Kong Juan
Published: January 2019

Aim. Vitamin D plays an important role in water and salt homeostasis. The aim of our study was to investigate the underlying relationship of Vitamin D and Aquaporins (AQP). Methods. The behaviors of 1α (OH)-ase knockout mice and wild type mice were observed before analysis. The ICR mice were treated with vehicle or paricalcitol, a vitamin D analogue, followed by animals receiving a standard diet and free access to drinking water either with aliskiren (renin blocker; 37.5 mg aliskiren in 100 ml water), or telmisartan (a angiotensin II type I receptor blocker; 40 mg telmisartan in 100 ml water) a week before study. The expressions of AQP-1, AQP-4, and renin in mice kidneys were detected by western bolting, immunohistochemistry, and immunofluorescence. Results. Diuresis and polydipsia were observed in 1α (OH)-ase knockout mice, and a decreased water intake and urine output in ICR mice was observed after paricalcitol treatment. Compared with wild type, the AQP-1 expressions were increased in renal papilla and AQP-4 expressions were decreased in renal proximal tubule of 1α(OH) ase knockout mice. In addition, AQP-1 was decreased in renal papilla and AQP-4 expressions were increased in proximal tubule by suppressing renin activity or supplement of Vitamin D analogue. After injecting renin into the lateral ventricle of the 1α(OH)ase knockout mice, the renin expression level was decreased in the kidney, followed by the decrease of AQP-1 in renal papilla and increase of AQP-4 in proximal tubule. Conclusions. Overall, Vitamin D and renin inhibitors have synergistic effects in regulating water channels in mice kidneys.
An Application of the Newton‐Homotopy Continuation Method for Solving the Forward Kinematic Problem of the 3‐RRS Parallel Manipulator
Authors: Gallardo-Alvarado Jaime
Published: January 2019

The forward kinematic problem (FKP) of the 3‐RRS parallel manipulator is solved by means of the Newton‐homotopy continuation method. The closure equations are formulated in the three‐dimensional Euclidean spaces considering the coordinates of the centers of the spherical joints as unknown variables. The method is easy to follow and unlike the classical Newton‐Raphson method it allows finding all the solutions of the FKP. A case study is included in the contribution in order to confirm the correctness of the method. In that concern, the numerical results obtained by means of the proposed method are verified with the aid of two different approaches such as the application of commercially available software like Maple16™ and the application of the PHCpack, a general purpose solver for polynomial systems based on homotopy continuation.
Hybrid Approach of Finite Element Method, Kigring Metamodel, and Multiobjective Genetic Algorithm for Computational Optimization of a Flexure Elbow Joint for Upper‐Limb Assistive Device
Authors: Nguyen Duc Nam , Dao Thanh-Phong , Chau Ngoc Le , Dang Van Anh
Published: January 2019

Modeling for robotic joints is actually complex and may lead to wrong Pareto‐optimal solutions. Hence, this paper develops a new hybrid approach for multiobjective optimization design of a flexure elbow joint. The joint is designed for the upper‐limb assistive device for physically disable people. The optimization problem considers three design variables and two objective functions. An efficient hybrid optimization approach of central composite design (CDD), finite element method (FEM), Kigring metamodel, and multiobjective genetic algorithm (MOGA) is developed. The CDD is used to establish the number of numerical experiments. The FEM is developed to retrieve the strain energy and the reaction torque of joint. And then, the Kigring metamodel is used as a black‐box to find the pseudoobjective functions. Based on pseudoobjective functions, the MOGA is applied to find the optimal solutions. Traditionally, an evolutionary optimization algorithm can only find one Pareto front. However, the proposed approach can generate 6 Pareto‐optimal solutions, as near optimal candidates, which provides a good decision‐maker. Based on the user’s real‐work problem, one of the best optimal solutions is chosen. The results found that the optimal strain energy is about 0.0033 mJ and the optimal torque is approximately 588.94 Nm. Analysis of variance is performed to identify the significant contribution of design variables. The sensitivity analysis is then carried out to determine the effect degree of each parameter on the responses. The predictions are in a good agreement with validations. It confirms that the proposed hybrid optimization approach has an effectiveness to solve for complex optimization problems.
Eco-Friendly Approach and Potential Biodegradable Polymer Matrix for WPC Composite Materials in Outdoor Application
Authors: Quitadamo Alessia , Massardier Valerie , Valente Marco
Published: January 2019

Blends based on high-density polyethylene (HDPE) and poly(lactic) acid (PLA) with different ratios of both polymers were produced: a blend with equal amounts of HDPE and PLA, hence 50 wt.% each, proved to be a useful compromise, allowing a high amount of bioderived charge without this being too detrimental for mechanical properties and considering its possibility to biodegradation behaviour in outdoor application. In this way, an optimal blend suitable for producing a composite with cellulosic fillers is proposed. In the selected polymer blend, wood flour (WF) was added as a natural filler in the proportion of 20, 30, and 40 wt.%, considering as 100 the weight of the polymer blend matrix. There are two compatibilizers to modify both HDPE-PLA blend and wood-flour/polymer interfaces, i.e., polyethylene-grafted maleic anhydride and a random copolymer of ethylene and glycidyl methacrylate. The most suitable percentage of compatibilizer for HDPE-PLA blends appears to be 3 wt.%, which was selected also for use with wood flour. In order to evaluate properties of blends and composites tensile tests, scanning electron microscopy, differential scanning calorimetry, thermogravimetric analyses, and infrared spectroscopy have been performed. Wood flour seems to affect heavy blend behaviour in process production of material suggesting that future studies are needed to reduce defectiveness.
Inhibition of LOXL2 Enhances the Radiosensitivity of Castration-Resistant Prostate Cancer Cells Associated with the Reversal of the EMT Process
Authors: Xie Peng , Yu Hongliang , Wang Feijiang , Yan Feng , He Xia
Published: January 2019

Introduction. Radiotherapy is the mainstay in the treatment of prostate cancer. However, significant radioresistance of castration-resistant prostate cancer (CRPC) cells constitutes a main obstacle in the treatment of this disease. By using bioinformatic data mining methods, LOXL2 was found to be upregulated in both androgen-independent prostate cancer cell lines and radioresistant tumor samples collected from patients with prostate cancer. We speculate that LOXL2 may play an important role in the radioresistance of CRPC cells. Methods. The effect of LOXL2 knockdown on the radiosensitivity of androgen-independent prostate cancer cells lines was measured by the clonogenic assay and xenograft tumor experiments under in vitro and in vivo conditions, respectively. In studies on the mechanism, we focused on the EMT phenotype changes and cell apoptosis changes induced by LOXL2 knockdown in DU145 cells. The protein levels of three EMT biomarkers, namely, E-cadherin, vimentin, and N-cadherin, were measured by western blotting and immunohistochemical staining. Cell apoptosis after irradiation was measured by flow cytometry and caspase-3 activity assay. Salvage experiment was also conducted to confirm the possible role of EMT in the radiosensitization effect of LOXL2 knockdown in CRPC cells. Results. LOXL2 knockdown in CRPC cells enhanced cellular radiosensitivity under both in vitro and in vivo conditions. A significant reversal of EMT was observed in LOXL2-silenced DU145 cells. Cell apoptosis after irradiation was significantly enhanced by LOXL2 knockdown in DU145 cells. Results from the salvage experiment confirmed the key role of EMT process reversal in the radiosensitization effect of LOXL2 knockdown in DU145 cells. Conclusions. LOXL2 plays an important role in the development of cellular radioresistance in CRPC cells. Targeting LOXL2 may be a rational avenue to overcome radioresistance in CRPC cells. A LOXL2-targeting strategy for CRPC treatment warrants detailed investigation in the future.
Fabrication and Characterization of Chitosan–Vitamin C–Lactic Acid Composite Membrane for Potential Skin Tissue Engineering
Authors: Madni Ahmed , Khan Romana , Ikram Muhammad , Naz Syeda Sohaila , Khan Taous , Wahid Fazli
Published: January 2019

Recent advances in tissue engineering have potential for the development of improved substitutes for damaged skin tissues. Vitamin C and lactic acid are well-known wound healing accelerators while chitosan is an important biomaterial having wound healing capabilities. However, addition of vitamin C induces fragility to the chitosan–lactic acid membranes. Therefore, the current study was designed to fabricate an intact chitosan–vitamin C–lactic acid composite membrane that may synergize the critical properties of every individual component for potential skin tissue engineering. For this purpose, different concentrations of glycerol and polyethylene glycol (PEG) were added to strengthen the chitosan–vitamin C–lactic acid membranes. The prepared membranes were characterized by Fourier transform infrared spectroscopy, X–ray diffraction, and field emission scanning electron microscopy. Moreover, the biocompatibility of the prepared membranes was evaluated with fibroblast NIH 3T3 cells. The results showed that addition of glycerol and PEG has improved the strength of chitosan–vitamin C–lactic acid composite membrane. Characterization studies revealed the successful synthesis of chitosan–vitamin C–lactic acid composite membrane. Moreover, the prepared membranes showed excellent biocompatibility with NIH 3T3 cells. However, it is important to note that cells showed more attachment and spreading on porous chitosan composites membranes as compared to nonporous membranes. This study provided a base for the development of an intact chitosan–vitamin C–lactic acid composite membrane for skin tissue engineering. However, further preclinical and clinical studies are required for its practical applications in skin tissue engineering.
Predicting Intracerebral Hemorrhage Patients’ Length-of-Stay Probability Distribution Based on Demographic, Clinical, Admission Diagnosis, and Surgery Information
Authors: Luo Li , Xu Xueru , Jiang Yan , Zhu Wei
Published: January 2019

The vast majority of patients with intracerebral hemorrhage (ICH) suffer from long and uncertain length of stay (LOS). The aim of our study was to provide decision support for discharge and admission plans by predicting ICH patients’ LOS probability distribution. The demographics, clinical predictors, admission diagnosis, and surgery information from 3,600 ICH patients were used in this study. We used univariable Cox analysis, multivariable Cox analysis, Cox-variable of importance (Cox-VIMP) analysis, and an intersection analysis to select predictors and used random survival forests (RSF)—a method in survival analysis—to predict LOS probability distribution. The Cox-VIMP method constructed by us effectively selected significant correlation predictors. The Cox-VIMP RSF model can improve prediction performance and is significantly different from the other models. The Cox-VIMP can contribute to the screening of predictors, and the RSF model can be established through those predictors to predict the probability distribution of LOS in each patient.
Control of a Humanoid NAO Robot by an Adaptive Bioinspired Cerebellar Module in 3D Motion Tasks
Authors: Antonietti Alberto , Martina Dario , Casellato Claudia , D’Angelo Egidio , Pedrocchi Alessandra
Published: January 2019

A bioinspired adaptive model, developed by means of a spiking neural network made of thousands of artificial neurons, has been leveraged to control a humanoid NAO robot in real time. The learning properties of the system have been challenged in a classic cerebellum-driven paradigm, a perturbed upper limb reaching protocol. The neurophysiological principles used to develop the model succeeded in driving an adaptive motor control protocol with baseline, acquisition, and extinction phases. The spiking neural network model showed learning behaviours similar to the ones experimentally measured with human subjects in the same task in the acquisition phase, while resorted to other strategies in the extinction phase. The model processed in real-time external inputs, encoded as spikes, and the generated spiking activity of its output neurons was decoded, in order to provide the proper correction on the motor actuators. Three bidirectional long-term plasticity rules have been embedded for different connections and with different time scales. The plasticities shaped the firing activity of the output layer neurons of the network. In the perturbed upper limb reaching protocol, the neurorobot successfully learned how to compensate for the external perturbation generating an appropriate correction. Therefore, the spiking cerebellar model was able to reproduce in the robotic platform how biological systems deal with external sources of error, in both ideal and real (noisy) environments.
Association between the Number of Childbirths and the Progress of Atherosclerosis among Women with Diabetes: A Cohort Study Based on Chinese Population
Authors: Wang Jie , Pei Yu , Chen Kang , Yan Wenhua , Wang Anping , Li Yijun , Li Jia , Wang Haibing , An Ping , Zhang Linxi , Ye Yingnan , Jin Xinye , Ning Guang , Mu Yiming , Gu Weijun
Published: January 2019

Background. The aim of this study is to explore the association between the number of childbirths and the progress of atherosclerosis among Chinese women with hypertension or diabetes. Methods. In total, 1159 Chinese parous women from a community longitudinal survey conducted in the communities of Shijingshan district, Beijing, China, were included in our study. They were divided into three groups according to the number of childbirths, and the change in pulse wave velocity (PWV) was as an indicator of the progression of atherosclerosis because the increased PWV reflected the more serious atherosclerosis. After 3 years, we conducted follow-up visits to the subjects. Logistical regression analyses were applied to investigate the relationship between the number of childbirths and the progression of atherosclerotic stiffness and a stratification analysis was performed for history of hypertension and diabetes. Results. After 3-year follow-up, among women with diabetes, the OR of women with 2 childbirths was significant [3.5 (95% confidence interval 1.5, 7.9)] in model I, [3.1 (95% confidence interval 1.3, 7.2)] in model II, and the OR of women with ≥3 childbirths was significant [4.4 (95% confidence interval 1.3, 14.5)] in model I, [4.1 (95% confidence interval 1.2, 14.3)] in model II. Among women with hypertension, the risk of the progress of atherosclerosis was not significant. Conclusion. The increasing number of childbirths is associated with the progression of atherosclerotic stiffness among Chinese women with diabetes, independent of a variety of confounding factors.
Evaluation of Skin Irritation and Acute and Subacute Oral Toxicity of Lavandula angustifolia Essential Oils in Rabbit and Mice
Authors: Mekonnen Awol , Tesfaye Solomon , Christos Selam G. , Dires Kassahun , Zenebe Tizazu , Zegeye Nigus , Shiferaw Yoseph , Lulekal Ermias
Published: January 2019

Lavandula angustifolia is used in traditional and folk medicines of Ankober District, North Central Ethiopia, for the treatment of several livestock and human disorders. This toxicity study aimed to investigate L. angustifolia essential oil oral toxicity in mice and skin irritation in rabbit. L. angustifolia essential oil was analyzed using gas chromatography-mass spectrometry methods and showed predominance of Eucalyptol (52.36%), Camphor (11.91%), gamma-terpinene (8.775%) and endoborneol (7.585%). Limit test at 2000 mg/kg dose was used for L. angustifolia essential oil acute toxicity test and revealed LD50 value was higher than 2000 mg/kg. For subacute toxicity study 2000mg/kg was given orally to each mouse for 21 days. The result demonstrated no significant changes (p > 0.05) in the body weights, and biochemical parameters, gross abnormalities, water, and food intake were observed. No macroscopic changes were seen in the histopathology analysis of kidneys and livers. For skin irritation test shaved rabbit skin was treated with 10% ointment formulation. Ointment of L. angustifolia oil did not affect mice skin. Generally, this toxicity study demonstrated that L. angustifolia essential oil is nontoxic.
Effects of Habitat Change on the Web Characteristics and Fitness of the Giant Wood Spider (Nephila pilipes) in Sri Lanka
Authors: Wijerathna Tharaka , Tharanga Dilini , Perera Inoka C. , Wijesinghe Mayuri R.
Published: January 2019

We compare web properties and fitness of the Giant wood spiderNephila pilpeswithin and outside its natural rainforest habitat in Sri Lanka. The nonforest habitats comprised rural home gardens and plantations. We hypothesize that marked differences would be evident between the two habitats in (i) web properties and (ii) fitness of the female spiders. Web architectural and silk thread properties were measured in 25 webs of adult female spiders in each of the two habitats, while female abdomen size was used as the proxy for fitness. Findings support both hypotheses. The nonforest webs were more closely knit (smaller mesh spaces) and the hub was placed at higher position on the web than that in the forest webs both altering prey capture efficiency. Also, females in nonforest habitats were significantly smaller than those in the forest, indicating lowered fitness. The disparities in web characteristics and fitness are impressive given that the forest and nonforest habitats are located in close proximity, suggesting that rainforest orbweaver spiders such asNephila pilpesmay suffer population declines if the extents of natural forest continue to shrink.
Simultaneous Bilateral Primary Spontaneous Pneumothorax: A Case Report and a Review of the Literature
Authors: Kewcharoen Jakrin , Morris Paul , Kanitsoraphan Chanavuth , La Hanh , Sriratanaviriyakul Narin
Published: January 2019

Background. Simultaneous bilateral primary spontaneous pneumothorax (SBPSP) is an extremely rare and potentially fatal condition. Patients usually have no relevant medical conditions. Some cases, however, may have certain risk factors such as smoking, being young, and male gender. We reported a case of a healthy young male who presented with BPSP. Case Presentation. A 21-year-old man with a past medical history of well-controlled intermittent asthma presented with acute worsening shortness of breath overnight. Chest X-ray performed showed bilateral large pneumothorax with significantly compressed mediastinum. Chest tubes were placed bilaterally with immediate clinical improvement. However, the chest tubes continued to have an air leak without full lungs expansion. Computed tomography scan without contrast of the chest revealed subpleural blebs in both upper lobes. The patient underwent bilateral video-assisted thoracoscopic surgery (VATS) with apical bleb resection, bilateral pleurectomy, and bilateral doxycycline pleurodesis. Biopsy of the apical blebs and parietal pleura of both lungs were negative for any atypical cells suspicious for malignancy or Langerhans cell histiocytosis. The patient had been doing well six months following surgery with no recurrence of pneumothorax. Conclusion. SBPSP is a rare and urgent condition that requires prompt intervention. In a young patient without any underlying disease, surgical intervention, such as VATS, is relatively safe and can be considered early.
The Difficult Airway Trolley: A Narrative Review and Practical Guide
Authors: Bjurström Martin F. , Bodelsson Mikael , Sturesson Louise W.
Published: January 2019

Death and severe morbidity attributable to anesthesia are commonly associated with failed difficult airway management. When an airway emergency develops, immediate access to difficult airway equipment is critical for implementation of rescue strategies. Previously, national expert consensus guidelines have provided only limited guidance for the design and setup of a difficult airway trolley. The overarching aim of the current work was to create a dedicated difficult airway trolley (for patients>12 years old) for use in anesthesia theatres, intensive care units, and emergency departments. A systematic literature search was performed, using the PubMed, Embase, and Google Scholar search engines. Based on evidence presented in 11 national or international guidelines, and peer-reviewed journals, we present and outline a difficult airway trolley organized to accommodate sequential progression through a four-step difficult airway algorithm. The contents of the top four drawers correspond to specific steps in the airway algorithm (A = intubation, B = oxygenation via a supraglottic airway device, C = facemask ventilation, and D = emergency invasive airway access). Additionally, specialized airway equipment may be included in the fifth drawer of the proposed difficult airway trolley, thus enabling widespread use. A logically designed, guideline-based difficult airway trolley is a vital resource for any clinician involved in airway management and may aid the adherence to difficult airway algorithms during evolving airway emergencies. Future research examining the availability of rescue airway devices in various clinical settings, and simulation studies comparing different types of difficult airway trolleys, are encouraged.
Neuroprotective Effects of Collagen-Glycosaminoglycan Matrix Implantation following Surgical Brain Injury
Authors: Chen Jia-Hui , Hsu Wei-Cherng , Huang Kuo-Feng , Hung Chih-Huang
Published: January 2019

Background. Neurological deficits following neurosurgical procedures are inevitable; however, there are still no effective clinical treatments. Earlier reports revealed that collagen-glycosaminoglycan (CG) matrix implantation promotes angiogenesis, neurogenesis, and functional recovery following surgical brain injury (SBI). The present study was conducted to further examine the potential neuroprotective effects of collagen-glycosaminoglycan (CG) matrix implantation following neurosurgery. Methods. CG implantation was performed in the lesion cavity created by surgical trauma. The Sprague-Dawley rat model of SBI was used as established in the previous study by the author. The rats were divided into three groups as follows: (1) sham (SHAM), (2) surgery-induced lesion cavity (L), and (3) CG matrix implantation following surgery-induced lesion cavity (L+CG). Proinflammatory (tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells)) and anti-inflammatory (IL-10 and granulocyte-macrophage colony-stimulating factor (GMCSF)) cytokine expression was evaluated by enzyme-linked immunosorbent assays. Microglial activation was evaluated by immunohistochemistry, and the neuroprotective effect of CG matrix implantation was evaluated by an immunohistochemical study of microglia ED-1 and IBA-1 (activated microglia) and myeloperoxidase (MPO) and by the analysis of IL-6, IL-10, TNF-α, NF-κB, and GMCSF cytokine levels. Apoptosis was also assessed using a TUNEL assay. Results. The results showed that CG matrix implantation following surgically induced lesions significantly decreased the density of ED-1, IBA-1, and MPO (activated microglia). The tissue concentration of proinflammatory cytokines, such as TNF-α, IL-6, and NF-κB was significantly decreased. Conversely, the anti-inflammatory cytokines GMCSF and IL-10 were significantly increased. Conclusions. Implantation of the CG matrix following SBI has neuroprotective effects, including the suppression of microglial activation and the production of inflammatory-related cytokines.
Long-Term Effects of Periodical Fires on Archaeal Communities from Brazilian Cerrado Soils
Authors: Belmok Aline , Rodrigues-Oliveira Thiago , Lopes Fabyano A. C. , Miranda Heloisa S. , Krüger Ricardo H. , Kyaw Cynthia M.
Published: January 2019

The Cerrado biome corresponds to an extensive area of Brazil and is considered a biodiversity hotspot. Frequent fires are a natural feature in this biome and have influences on vegetation structure and composition. However, continuous anthropogenic actions are promoting changes in fire frequency and seasonality. Despite the high biodiversity of the Cerrado, little is known about its microbiome, with few publications describing some aspects of the bacterial and fungal communities found on this biome and almost no references about archaea. In this study, we describe the archaeal diversity in Cerradosensu strictosoils, comparing the archaeal communities from soils of an area long protected from fires to one exposed to biennial fires, using both 16S rRNA andamoAgenes as molecular markers. Almost all 16S rRNA sequences from both studied areas were affiliated with I.1b and 1.1cThaumarchaeota, groups commonly detected in terrestrial environments. A higher relative abundance of I.1b thaumarchaeal subgroup was detected in the frequently burned area even though no statistically significant differences were observed in archaeal 16S rRNA richness and diversity between the investigated areas. Many ammonia-oxidizing archaea (AOA) are affiliated with this group, which is consistent with the higheramoAdiversity and OTU numbers detected in the area periodically burned. Taken together, our results suggest that, although total archaeal community richness and diversity do not seem to greatly differ between the investigated conditions, alterations in wood cover and vegetation structure caused by frequent fires likely cause long-term effects in AOA diversity in Cerrado soils.
Urban Water Ecosystem Health Evaluation Based on the Improved Fuzzy Matter‐Element Extension Assessment Model: Case Study from Zhengzhou City, China
Authors: Han Han , Li Huimin , Zhang Kaize
Published: January 2019

The urban water ecosystem is a core foundation for the urban construction of ecological civilization. Ecosystem health is directly related to the economic development of the region and the quality of urban residents’ lives. Evaluating the health state of an urban water ecosystem is an important prerequisite for the construction of an ecologically civilized city. This study used Zhengzhou City in China as research area. Firstly, a Pressure‐State‐Response (PSR) model was used to construct an urban water ecosystem evaluation index system. Secondly, after analyzing the deficiency of traditional fuzzy matter‐element extension model in urban water ecosystem health assessment, an improved fuzzy matter‐element extension assessment model (FMEAM) was constructed by introducing the variable weight theory. Finally, using the proposed model above and the data from 2007 to 2016, this study evaluated the water ecosystem health status of Zhengzhou based on water ecosystem health integrated index (WHI). The results showed that the state of the urban water ecosystem in Zhengzhou generally improved during the study period. However, the overall level still remained low and retained an unhealthy state throughout the timeframe. The pressure on the Zhengzhou water ecosystem mainly originates from the increasing urbanization rate, the increased consumption of ecological environmental resources, and household water. Corresponding countermeasures in Zhengzhou have achieved certain results, and the health of the state’s subsystem and response subsystem has improved. However, it remains necessary to further strengthen the protection of the ecological environment, particularly with respect to water resources.
Spot-Centroid Determination Algorithms in Semiactive Laser Photodiodes for Artillery Applications
Authors: de Celis Raul , Cadarso Luis
Published: January 2019

Precision of guided projectiles depends equally on the accuracy in determining the coordinates of the objective and on the exactness of the measurement devices utilized for position and attitude calculation of the projectile. Development of algorithms for low-cost high-precision terminal guidance systems is a cornerstone in research in this field. Semiactive laser (SAL) kits, and particularly quadrant detector devices, have been developed to improve precision in guided weapons. Photodetection system can be functionally divided into two main parts: sensing and processing. The sensed signal is processed to estimate the spot coordinates, i.e., the laser footprint, which provides some information regarding projectile-target relative position, to obtain the needed information for the navigation and guidance algorithms. The electrical intensities that a real sensor provides under laboratory conditions are compared to a mathematical model based on area intersection calculations to simulate the intensities on real flights. Then, four different processing algorithms, two of them rational, and the other two logarithmic, are tested for different spot sizes, which are nonlinear. Proposing an interpolation algorithm based on the four electrical intensities obtained in a semiactive laser quadrant photodetector, laser footprint center estimation is improved for artillery applications. Finally, an example illustrating a projectile flight is employed to compare real and calculated laser footprints in order to select the best algorithm for artillery applications.