bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Nurse Staffing on Patient Outcomes in Acute Care Hospitals in Korea
Authors: Yoon Hyo-Jeong
Published: November 2022

Nurse staffing is an important factor influencing patient health outcomes. This study aimed to analyze the effects of nurse staffing on patient health outcomes, such as length of stay, mortality within 30 days of hospitalization, and readmission within 7 days of discharge, in acute care hospitals in Korea. Data from the first quarter of 2018 were collected using public and inpatient sample data from the Health Insurance Review and Assessment Service. The data of 46,196 patients admitted to 536 general wards of acute care hospitals were analyzed. A multilevel logistic analysis was performed for the patients’ mortality and early readmission, and a multilevel zero-truncated negative binomial analysis was performed for the length of stay. The average length of stay in acute care hospitals was 6.54 ± 6.03 days, the mortality rate was 1.1%, and the early readmission rate was 7.1%. As the nurse staffing level increased, the length of stay and number of early readmissions were likely to decrease. It can be concluded that interventions to improve nurse staffing are required; for example, a policy that compels medical institutions to comply with Korea’s medical law standards should be implemented. Additionally, continuous research and interventions are needed to establish an appropriate nurse staffing level according to patient severity.
Survey on Reporting of Child Abuse by Pediatricians: Intrapersonal Inconsistencies Influence Reporting Behavior More than Legislation
Authors: Berthold Oliver , Clemens Vera , Levi Benjamin H. , Jarczok Marion , Fegert Jörg M. , Jud Andreas
Published: November 2022

Background: Internationally, various laws govern reporting of child abuse to child protection services by medical professionals. Whether mandatory reporting laws are in place or not, medical professionals need internal thresholds for suspicion of abuse to even consider a report (“reasonable suspicion” in US law, “gewichtige Anhaltspunkte” in German law). Objective: To compare internal thresholds for suspicion of abuse among US and German pediatricians, i.e., from two countries with and without mandatory reporting laws. Participants and Setting: In Germany, 1581 pediatricians participated in a nationwide survey among child health professionals. In the US, a survey was mailed to all Pennsylvania pediatricians, and 1249 participated. Methods: Both samples were asked how high in their rank order of differential diagnoses child abuse would have to be when confronted with a child’s injuries to qualify for reasonable suspicion/gewichtige Anhaltspunkte (differential diagnosis scale, DDS). In a second step, both had to mark a 10-point likelihood scale (0–100%) corresponding to reasonable suspicion/gewichtige Anhaltspunkte (estimated probability scale, EPS). Results: While for almost two-thirds of German pediatricians (62.4%), child abuse had to be among the top three differential diagnoses for gewichtige Anhaltspunkte, over half of the US respondents (48.1%) had a lower threshold for reasonable suspicion. On the estimated probability scale, over 65% in both samples indicated that the probability of abuse had to exceed 50% for reasonable suspicion/gewichtige Anhaltspunkte. There was great variability between the two countries. Conclusions: There are similar uncertainties in assessing cases of suspected child abuse in different legal systems. There is a need for debates on thresholds among medical professionals in both countries.
Beneficial Effects of Melatonin on Periodontitis Management: Far More Than Oral Cavity
Authors: Wang Chuan , Wang Leilei , Wang Xiaoxuan , Cao Zhengguo
Published: November 2022

Periodontitis as a highly prevalent chronic infection/inflammatory disease can eventually lead to tooth loss and masticatory dysfunction. It also has a negative impact on general health and largely impairs quality of life. The tissue destruction during periodontitis is mainly caused by the excessive immune–inflammatory response; hence, how to modulate the host’s reaction is of profound importance for effective periodontal treatment and tissue protection. Melatonin, as an endogenous hormone exhibiting multiple biological functions such as circadian rhythm regulation, antioxidant, and anti-inflammation, has been widely used in general healthcare. Notably, the past few years have witnessed increasing evidence for the application of melatonin as an adjunctive approach in the treatment of periodontitis and periodontitis-related systemic comorbidities. The detailed underlying mechanisms and more verification from clinical practice are still lacking, however, and further investigations are highly required. Importantly, it is essential to establish standard guidelines in the near future for the clinical administration of melatonin for periodontal health and general wellbeing.
Microgreens Biometric and Fluorescence Response to Iron (Fe) Biofortification
Authors: Frąszczak Barbara , Kleiber Tomasz
Published: November 2022

Microgreens are foods with high nutritional value, which can be further enhanced with biofortification. Crop biofortification involves increasing the accumulation of target nutrients in edible plant tissues through fertilization or other factors. The purpose of the present study was to evaluate the potential for biofortification of some vegetable microgreens through iron (Fe) enrichment. The effect of nutrient solution supplemented with iron chelate (1.5, 3.0 mg/L) on the plant’s growth and mineral concentration of purple kohlrabi, radish, pea, and spinach microgreens was studied. Increasing the concentration of Fe in the medium increased the Fe content in the leaves of the species under study, except for radish. Significant interactions were observed between Fe and other microelements (Mn, Zn, and Cu) content in the shoots. With the increase in the intensity of supplementation with Fe, regardless of the species, the uptake of zinc and copper decreased. However, the species examined suggested that the response to Fe enrichment was species-specific. The application of Fe didn’t influence plant height or fresh and dry weight. The chlorophyll content index (CCI) was different among species. With increasing fertilisation intensity, a reduction in CCI only in peas resulted. A higher dose of iron in the medium increased the fluorescence yield of spinach and pea microgreens. In conclusion, the tested species, especially spinach and pea, grown in soilless systems are good targets to produce high-quality Fe biofortified microgreens.
Potential of Non-Coding RNA as Biomarkers for Progressive Supranuclear Palsy
Authors: Simoes Fabio A. , Joilin Greig , Peters Oliver , Schneider Luisa-Sophie , Priller Josef , Spruth Eike Jakob , Vogt Ina , Kimmich Okka , Spottke Annika , Hoffmann Daniel C. , Falkenburger Björn , Brandt Moritz , Prudlo Johannes , Brockmann Kathrin , Fries Franca Laura , Rowe James B. , Church Alistair , Respondek Gesine , Newbury Sarah F. , Leigh P. Nigel , Morris Huw R. , Höglinger Günter U. , Hafezparast Majid
Published: November 2022

Objective markers for the neurodegenerative disorder progressive supranuclear palsy (PSP) are needed to provide a timely diagnosis with greater certainty. Non-coding RNA (ncRNA), including microRNA, piwi-interacting RNA, and transfer RNA, are good candidate markers in other neurodegenerative diseases, but have not been investigated in PSP. Therefore, as proof of principle, we sought to identify whether they were dysregulated in matched serum and cerebrospinal fluid (CSF) samples of patients with PSP. Small RNA-seq was undertaken on serum and CSF samples from healthy controls (n = 20) and patients with PSP (n = 31) in two cohorts, with reverse transcription-quantitative PCR (RT-qPCR) to confirm their dysregulation. Using RT-qPCR, we found in serum significant down-regulation in hsa-miR-92a-3p, hsa-miR-626, hsa-piR-31068, and tRNA-ValCAC. In CSF, both hsa-let-7a-5p and hsa-piR-31068 showed significant up-regulation, consistent with their changes observed in the RNA-seq results. Interestingly, we saw no correlation in the expression of hsa-piR-31068 within our matched serum and CSF samples, suggesting there is no common dysregulatory mechanism between the two biofluids. While these changes were in a small cohort of samples, we have provided novel evidence that ncRNA in biofluids could be possible diagnostic biomarkers for PSP and further work will help to expand this potential.
Layered Double Hydroxides Derived from MIL-88A(Fe) as an Efficient Adsorbent for Enhanced Removal of Lead (II) from Water
Authors: Huo Jiang-Bo , Yu Guoce
Published: November 2022

The efficient removal of lead (II) from aqueous solution remains a big problem and the development of novel nanomaterials as adsorbents by various technologies to solve this problem is promising. This study contributed a novel nanostructure of MIL-88A-layered double hydroxides (LDHs) as the adsorbent for Pb2+, which was synthesized by a two-step solvothermal method with MIL-88A(Fe) as the precursor. The as-prepared material featured a chestnut-like core-shell structure, and exhibited excellent removal performance towards Pb2+ from water in comparison to MIL-88A(Fe) and LDHs (directly synthesized). The adsorption of Pb2+ by the MIL-88A-LDHs conformed to the pseudo-second-order kinetic model and the Langmuir and Freundlich isotherm models. The maximal adsorption capacity was 526.32, 625.00, and 909.09 mg g−1 at 278, 298, and 318 K, respectively. The thermodynamic parameters suggested that the adsorption was an endothermic, entropy-increasing, and spontaneous reaction. X-ray photoelectron spectroscopy (XPS) analysis indicated that the surface complexation was mostly responsible for Pb2+ elimination. The MIL-88A-LDHs can be readily regenerated and showed good cyclic performance towards Pb2+. Thus, the as-prepared MIL-88A-LDHs may hold promise for the elimination of aqueous heavy metals.
Multi-Trait Genomic Prediction Improves Accuracy of Selection among Doubled Haploid Lines in Maize
Authors: Hu Haixiao , Meng Yujie , Liu Wenxin , Chen Shaojiang , Runcie Daniel E.
Published: November 2022

Recent advances in maize doubled haploid (DH) technology have enabled the development of large numbers of DH lines quickly and efficiently. However, testing all possible hybrid crosses among DH lines is a challenge. Phenotyping haploid progenitors created during the DH process could accelerate the selection of DH lines. Based on phenotypic and genotypic data of a DH population and its corresponding haploids, we compared phenotypes and estimated genetic correlations between the two populations, compared genomic prediction accuracy of multi-trait models against conventional univariate models within the DH population, and evaluated whether incorporating phenotypic data from haploid lines into a multi-trait model could better predict performance of DH lines. We found significant phenotypic differences between DH and haploid lines for nearly all traits; however, their genetic correlations between populations were moderate to strong. Furthermore, a multi-trait model taking into account genetic correlations between traits in the single-environment trial or genetic covariances in multi-environment trials can significantly increase genomic prediction accuracy. However, integrating information of haploid lines did not further improve our prediction. Our findings highlight the superiority of multi-trait models in predicting performance of DH lines in maize breeding, but do not support the routine phenotyping and selection on haploid progenitors of DH lines.
Ketone Bodies as Metabolites and Signalling Molecules at the Crossroad between Inflammation and Epigenetic Control of Cardiometabolic Disorders
Authors: Bendridi Nadia , Selmi Anna , Balcerczyk Aneta , Pirola Luciano
Published: November 2022

For many years, it has been clear that a Western diet rich in saturated fats and sugars promotes an inflammatory environment predisposing a person to chronic cardiometabolic diseases. In parallel, the emergence of ketogenic diets, deprived of carbohydrates and promoting the synthesis of ketone bodies imitating the metabolic effects of fasting, has been shown to provide a possible nutritional solution to alleviating diseases triggered by an inflammatory environment. The main ketone body, β-hydroxybutyrate (BHB), acts as an alternative fuel, and also as a substrate for a novel histone post-translational modification, β-hydroxybutyrylation. β-hydroxybutyrylation influences the state of chromatin architecture and promotes the transcription of multiple genes. BHB has also been shown to modulate inflammation in chronic diseases. In this review, we discuss, in the pathological context of cardiovascular risks, the current understanding of how ketone bodies, or a ketogenic diet, are able to modulate, trigger, or inhibit inflammation and how the epigenome and chromatin remodeling may be a key contributor.
A Schematic Approach to Defining the Prevalence of COL VI Variants in Five Years of Next-Generation Sequencing
Authors: Marinella Gemma , Astrea Guja , Buchignani Bianca , Cassandrini Denise , Doccini Stefano , Filosto Massimiliano , Galatolo Daniele , Gallone Salvatore , Giannini Fabio , Lopergolo Diego , Maioli Maria Antonietta , Magri Francesca , Malandrini Alessandro , Mandich Paola , Mari Francesco , Massa Roberto , Mata Sabrina , Melani Federico , Moggio Maurizio , Mongini Tiziana E. , Pasquariello Rosa , Pegoraro Elena , Ricci Federica , Ricci Giulia , Rodolico Carmelo , Rubegni Anna , Siciliano Gabriele , Sperti Martina , Ticci Chiara , Tonin Paola , Santorelli Filippo M. , Battini Roberta
Published: November 2022

Objective: To define the prevalence of variants in collagen VI genes through a next-generation sequencing (NGS) approach in undiagnosed patients with suspected neuromuscular disease and to propose a diagnostic flowchart to assess the real pathogenicity of those variants. Methods: In the past five years, we have collected clinical and molecular information on 512 patients with neuromuscular symptoms referred to our center. To pinpoint variants in COLVI genes and corroborate their real pathogenicity, we sketched a multistep flowchart, taking into consideration the bioinformatic weight of the gene variants, their correlation with clinical manifestations and possible effects on protein stability and expression. Results: In Step I, we identified variants in COLVI-related genes in 48 patients, of which three were homozygous variants (Group 1). Then, we sorted variants according to their CADD score, clinical data and complementary studies (such as muscle and skin biopsy, study of expression of COLVI on fibroblast or muscle and muscle magnetic resonance). We finally assessed how potentially pathogenic variants (two biallelic and 12 monoallelic) destabilize COL6A1-A2-A3 subunits. Overall, 15 out of 512 patients were prioritized according to this pipeline. In seven of them, we confirmed reduced or absent immunocytochemical expression of collagen VI in cultured skin fibroblasts or in muscle tissue. Conclusions: In a real-world diagnostic scenario applied to heterogeneous neuromuscular conditions, a multistep integration of clinical and molecular data allowed the identification of about 3% of those patients harboring pathogenetic collagen VI variants.
Endogenous Modulation of Extracellular Matrix Collagen during Scar Formation after Myocardial Infarction
Authors: Schumacher David , Curaj Adelina , Staudt Mareike , Simsekyilmaz Sakine , Kanzler Isabella , Boor Peter , Klinkhammer Barbara Mara , Li Xiaofeng , Bucur Octavian , Kaabi Adnan , Xu Yichen , Zheng Huabo , Nilcham Pakhwan , Schuh Alexander , Rusu Mihaela , Liehn Elisa A.
Published: November 2022

Myocardial infarction is remains the leading cause of death in developed countries. Recent data show that the composition of the extracellular matrix might differ despite similar heart function and infarction sizes. Because collagen is the main component of the extracellular matrix, we hypothesized that changes in inflammatory cell recruitment influence the synthesis of different collagen subtypes in myofibroblasts, thus changing the composition of the scar. We found that neutrophils sustain the proliferation of fibroblasts, remodeling, differentiation, migration and inflammation, predominantly by IL-1 and PPARγ pathways (n = 3). They also significantly inhibit the mRNA expression of fibrillar collagen, maintaining a reduced stiffness in isolated myofibroblasts (n = 4–5). Reducing the neutrophil infiltration in CCR1−/− resulted in increased mRNA expression of collagen 11, moderate expression of collagen 19 and low expression of collagen 13 and 26 in the scar 4 weeks post infarction compared with other groups (n = 3). Mononuclear cells increased the synthesis of all collagen subtypes and upregulated the NF-kB, angiotensin II and PPARδ pathways (n = 3). They increased the synthesis of collagen subtypes 1, 3, 5, 16 and 23 but reduced the expression of collagens 5 and 16 (n = 3). CCR2−/− scar tissue showed higher levels of collagen 13 (n = 3), in association with a significant reduction in stiffness (n = 4–5). Upregulation of the inflammation-related genes in myofibroblasts mostly modulated the fibrillar collagen subtypes, with less effect on the FACIT, network-forming and globular subtypes (n = 3). The upregulation of proliferation and differentiation genes in myofibroblasts seemed to be associated only with the fibrillar collagen subtype, whereas angiogenesis-related genes are associated with fibrillar, network-forming and multiplexin subtypes. In conclusion, although we intend for our findings to deepen the understanding of the mechanism of healing after myocardial infarction and scar formation, the process of collagen synthesis is highly complex, and further intensive investigation is needed to put together all the missing puzzle pieces in this still incipient knowledge process.
Basophil Activation Test with Different Polyethylene Glycols in Patients with Suspected PEG Hypersensitivity Reactions
Authors: Vespa Simone , Del Biondo Pietro , Simeone Pasquale , Cavallucci Enrico , Catitti Giulia , Auciello Raffaella , De Bellis Domenico , Altomare Isotta , Pierdomenico Laura , Canonico Barbara , Cicalini Ilaria , Angilletta Ilaria , Del Boccio Piero , Pieragostino Damiana , Santilli Francesca , Urbani Andrea , De Laurenzi Vincenzo , Stuppia Liborio , Lanuti Paola
Published: November 2022

Allergic reactions to COVID-19 vaccine components are rare but should be considered. Polyethylene glycol (PEG) is responsible for anaphylaxis in mRNA vaccines. Skin tests have been used in the allergological work-up programs for COVID-19 vaccine evaluation. However, the reproducibility of the skin prick test is time-dependent and the reactivity declines over time. Therefore, we combined the administration of the skin tests with the basophil activation test (BAT) using PEG2000, PEG4000 and DMG-PEG2000, where the BAT was considered positive when the percentage of activated basophils was higher than 6%, 5% and 6.5%, for PEG 4000, PEG2000 and DMG-PEG2000, respectively. To this end, among the subjects that underwent allergy counseling at the Allergy Unit of our Institution during the 2020/2021 vaccination campaign, 13 patients had a suggested medical history of PEG/drug hypersensitivity and were enrolled together with 10 healthy donors. Among the enrolled patients 2 out of 13 tested patients were positive to the skin test. The BAT was negative in terms of the percentages of activated basophils in all analyzed samples, but the stimulation index (SI) was higher than 2.5 in 4 out of 13 patients. These data evidenced that, when the SI is higher than 2.5, even in the absence of positivity to BAT, the BAT to PEG may be a useful tool to be coupled to skin tests to evidence even low-grade reactions.
Salmonella-Based Biorodenticides: Past Applications and Current Contradictions
Authors: Shikov Anton E. , Belousova Maria E. , Belousov Mikhail V. , Nizhnikov Anton A. , Antonets Kirill S.
Published: November 2022

The idea of using pathogens to control pests has existed since the end of the 19th century. Enterobacteria from the genus Salmonella, discovered at that time, are the causative agents of many serious diseases in mammals often leading to death. Mostly, the strains of Salmonella are able to infect a wide spectrum of hosts belonging to vertebrates, but some of them show host restriction. Several strains of these bacteria have been used as biorodenticides due to the host restriction until they were banned in many countries in the second part of the 20th century. The main reason for the ban was their potential pathogenicity for some domestic animals and poultry and the outbreaks of gastroenteritis in humans. Since that time, a lot of data regarding the host specificity and host restriction of different strains of Salmonella have been accumulated, and the complexity of the molecular mechanisms affecting it has been uncovered. In this review, we summarize the data regarding the history of studying and application of Salmonella-based rodenticides, discuss molecular systems controlling the specificity of Salmonella interactions within its multicellular hosts at different stages of infection, and attempt to reconstruct the network of genes and their allelic variants which might affect the host-restriction mechanisms.
MIG-6 Is Critical for Progesterone Responsiveness in Human Complex Atypical Hyperplasia and Early-Stage Endometrial Cancer
Authors: Jeong Olivia , Broaddus Russell R. , Lessey Bruce A. , Risinger John I. , Hunter Mark I. , Kim Tae Hoon
Published: November 2022

Women with complex atypical hyperplasia (CAH) or early-stage endometrioid endometrial cancer (EEC) are candidates for fertility preservation. The most common approach is progesterone (P4) therapy and deferral of hysterectomy until after completion of childbearing. However, P4 therapy response rates vary, and molecular mechanisms behind P4 resistance are poorly understood. One potential molecular cause of P4 resistance is a loss or attenuation of PGR expression. Mitogen-inducible gene 6 (MIG-6) is critical for P4 responsiveness. MIG-6 protein expression in the endometrial epithelial and stromal cells from women with CAH and EEC was significantly lower compared to women without CAH or EEC. The P4-responsive women (10/15) exhibited an increase of MIG-6 expression in epithelial and stromal cells compared to P4-resistant women (5/15). In addition, immunohistochemical analysis for PGR results showed that stromal PGR levels are significantly higher in P4-responsive women compared to P4-resistant women, whereas epithelial PGR expression was not different. A reverse correlation of MIG-6 and pAKT levels was observed in early-stage EEC patients. Studies strongly suggest that loss of MIG-6 and PGR and activation of pAKT lead to P4 resistance in CAH and EEC. These results will help to elucidate the molecular mechanism leading to P4 resistance in CAH and EEC.
Inhibition of the MAPK/c-Jun-EGR1 Pathway Decreases Photoreceptor Cell Death in the rd1 Mouse Model for Inherited Retinal Degeneration
Authors: Dong Yujie , Xu Wenrong , Li Yan , Wei Chunling , Hu Yunzhang , Hu Zhulin , Paquet-Durand François , Jiao Kangwei
Published: November 2022

Retinitis pigmentosa (RP) is a group of inherited retinal dystrophies that typically results in photoreceptor cell death and vision loss. Here, we explored the effect of early growth response-1 (EGR1) expression on photoreceptor cell death in Pde6brd1 (rd1) mice and its mechanism of action. To this end, single-cell RNA-seq (scRNA-seq) was used to identify differentially expressed genes in rd1 and congenic wild-type (WT) mice. Chromatin immunoprecipitation (ChIP), the dual-luciferase reporter gene assay, and western blotting were used to verify the relationship between EGR1 and poly (ADP-ribose) polymerase-1 (PARP1). Immunofluorescence staining was used to assess PARP1 expression after silencing or overexpression of EGR1. Photoreceptor cell death was assessed using the TUNEL assay following silencing/overexpression of EGR1 or administration of MAPK/c-Jun pathway inhibitors tanzisertib and PD98059. Our results showed differential expression of ERG1 in rd1 and WT mice via scRNA-seq analysis. The ChIP assay demonstrated EGR1 binding to the PARP1 promoter region. The dual-luciferase reporter gene assay and western blotting results revealed that EGR1 upregulated PARP1 expression. Additionally, the TUNEL assay showed that silencing EGR1 effectively reduced photoreceptor cell death. Similarly, the addition of tanzisertib and PD98059 reduced the expression of c-Jun and EGR1 and decreased photoreceptor cell death. Our study revealed that inhibition of the MAPK/c-Jun pathway reduced the expression of EGR1 and PARP1 and prevented photoreceptor cell death. These results highlight the importance of EGR1 for photoreceptor cell death and identify a new avenue for therapeutic interventions in RP.
Preventing Surgery-Induced NK Cell Dysfunction Using Anti-TGF-β Immunotherapeutics
Authors: Market Marisa , Tennakoon Gayashan , Scaffidi Marlena , Cook David P. , Angka Leonard , Ng Juliana , Tanese de Souza Christiano , Kennedy Michael A. , Vanderhyden Barbara C. , Auer Rebecca C.
Published: November 2022

Natural Killer (NK) cell cytotoxicity and interferon-gamma (IFNγ) production are profoundly suppressed postoperatively. This dysfunction is associated with increased morbidity and cancer recurrence. NK activity depends on the integration of activating and inhibitory signals, which may be modulated by transforming growth factor-beta (TGF-β). We hypothesized that impaired postoperative NK cell IFNγ production is due to altered signaling pathways caused by postoperative TGF-β. NK cell receptor expression, downstream phosphorylated targets, and IFNγ production were assessed using peripheral blood mononuclear cells (PBMCs) from patients undergoing cancer surgery. Healthy NK cells were incubated in the presence of healthy/baseline/postoperative day (POD) 1 plasma and in the presence/absence of a TGF-β-blocking monoclonal antibody (mAb) or the small molecule inhibitor (smi) SB525334. Single-cell RNA sequencing (scRNA-seq) was performed on PBMCs from six patients with colorectal cancer having surgery at baseline/on POD1. Intracellular IFNγ, activating receptors (CD132, CD212, NKG2D, DNAM-1), and downstream target (STAT5, STAT4, p38 MAPK, S6) phosphorylation were significantly reduced on POD1. Furthermore, this dysfunction was phenocopied in healthy NK cells through incubation with rTGF-β1 or POD1 plasma and was prevented by the addition of anti-TGF-β immunotherapeutics (anti-TGF-β mAb or TGF-βR smi). Targeted gene analysis revealed significant decreases in S6 and FKBP12, an increase in Shp-2, and a reduction in NK metabolism-associated transcripts on POD1. pSmad2/3 was increased and pS6 was reduced in response to rTGF-β1 on POD1, changes that were prevented by anti-TGF-β immunotherapeutics. Together, these results suggest that both canonical and mTOR pathways downstream of TGF-β mediate phenotypic changes that result in postoperative NK cell dysfunction.
Novel Therapeutics for Treating Sleep Disorders: New Perspectives on Maydis stigma
Authors: Kim Ryeong-Eun , Mabunga Darine Froy , Kim Hee Jin , Han Seol-Heui , Kim Hahn Young , Shin Chan Young , Kwon Kyoung Ja
Published: November 2022

Sleep is a restorative period that plays a crucial role in the physiological functioning of the body, including that of the immune system, memory processing, and cognition. Sleep disturbances can be caused by various physical, mental, and social problems. Recently, there has been growing interest in sleep. Maydis stigma (MS, corn silk) is a female maize flower that is traditionally used as a medicinal plant to treat many diseases, including hypertension, edema, and diabetes. It is also used as a functional food in tea and other supplements. β-Sitosterol (BS) is a phytosterol and a natural micronutrient in higher plants, and it has a similar structure to cholesterol. It is a major component of MS and has anti-inflammatory, antidepressive, and sedative effects. However, the potential effects of MS on sleep regulation remain unclear. Here, we investigated the effects of MS on sleep in mice. The effects of MS on sleep induction were determined using pentobarbital-induced sleep and caffeine-induced sleep disruption mouse models. MS extracts decreased sleep latency and increased sleep duration in both the pentobarbital-induced sleep induction and caffeine-induced sleep disruption models compared to the positive control, valerian root extract. The butanol fraction of MS extracts decreased sleep latency time and increased sleep duration. In addition, β-sitosterol enhances sleep latency and sleep duration. Both MS extract and β-sitosterol increased alpha activity in the EEG analysis. We measured the mRNA expression of melatonin receptors 1 and 2 (MT1/2) using qRT-PCR. The mRNA expression of melatonin receptors 1 and 2 was increased by MS extract and β-sitosterol treatment in rat primary cultured neurons and the brain. In addition, MS extract increased the expression of clock genes including per1/2, cry1/2, and Bmal1 in the brain. MS extract and β-sitosterol increased the phosphorylation of ERK1/2 and αCaMKII. Our results demonstrate for the first time that MS has a sleep-promoting effect via melatonin receptor expression, which may provide new scientific evidence for its use as a potential therapeutic agent for the treatment and prevention of sleep disturbance.
Software Tool for Visualization and Validation of Protein Turnover Rates Using Heavy Water Metabolic Labeling and LC-MS
Authors: Deberneh Henock M. , Sadygov Rovshan G.
Published: November 2022

Metabolic stable isotope labeling followed by liquid chromatography coupled with mass spectrometry (LC-MS) is a powerful tool for in vivo protein turnover studies of individual proteins on a large scale and with high throughput. Turnover rates of thousands of proteins from dozens of time course experiments are determined by data processing tools, which are essential components of the workflows for automated extraction of turnover rates. The development of sophisticated algorithms for estimating protein turnover has been emphasized. However, the visualization and annotation of the time series data are no less important. The visualization tools help to validate the quality of the model fits, their goodness-of-fit characteristics, mass spectral features of peptides, and consistency of peptide identifications, among others. Here, we describe a graphical user interface (GUI) to visualize the results from the protein turnover analysis tool, d2ome, which determines protein turnover rates from metabolic D2O labeling followed by LC-MS. We emphasize the specific features of the time series data and their visualization in the GUI. The time series data visualized by the GUI can be saved in JPEG format for storage and further dissemination.
Biocompatibility and Biological Performance of Additive-Manufactured Bioabsorbable Iron-Based Porous Interference Screws in a Rabbit Model: A 1-Year Observational Study
Authors: Tai Chien-Cheng , Huang Yu-Min , Liaw Chen-Kun , Yang Kuo-Yi , Ma Chun-Hsien , Huang Shin-I , Huang Chih-Chieh , Tsai Pei-I , Shen Hsin-Hsin , Sun Jui-Sheng , Chen Chih-Yu
Published: November 2022

This study evaluated the mid-term (12-month) biomechanical, biocompatibility, and biological performance of additive-manufactured bioabsorbable iron-based interference screws (ISs). Two bioabsorbable iron IS types—manufactured using pure iron powder (iron_IS) and using pure iron powder with 0.2 wt% tricalcium phosphate (TCP_IS)—were compared with conventional metallic IS (control) using in vitro biocompatibility and degradation analyses and an in vivo animal study. The in vitro ultimate failure strength was significantly higher for iron_IS and TCP_IS than for control ISs at 3 months post-operatively; however, the difference between groups were nonsignificant thereafter. Moreover, at 3 months after implantation, iron_IS and TCP_IS increased bone volume fraction, bone surface area fraction, and percent intersection surface; the changes thereafter were nonsignificant. Iron_IS and TCP_IS demonstrated degradation over time with increased implant surface, decreased implant volume, and structure thickness; nevertheless, the analyses of visceral organs and biochemistry demonstrated normal results, except for time-dependent iron deposition in the spleen. Therefore, compared with conventional ISs, bioabsorbable iron-based ISs exhibit higher initial mechanical strength. Although iron-based ISs demonstrate high biocompatibility 12 months after implantation, their corrosive iron products may accumulate in the spleen. Because they demonstrate mechanical superiority along with considerable absorption capability after implantation, iron-based ISs may have potential applications in implantable medical-device development in the future.
Effect of Sweep on Axial Fan Noise Sources Using the Lattice Boltzmann Method
Authors: Ghodake Dipali , Sanjosé Marlène , Moreau Stéphane , Henner Manuel
Published: November 2022

The effect of blade sweep has been studied numerically with the Lattice Boltzmann Method on a family of low-speed free-vortex axial fans with sweeps of ±45°. Good overall aerodynamic agreement is first demonstrated on all fans at the design condition, particularly in the tip gap. The local larger wall-pressure fluctuations seen in the unswept and backward swept fans compared to the forward case are traced to the stronger tip vortices that remain in the rotational plane or even move upstream. These stronger and faster vortices interacting with the fan blades are then responsible for the larger noise levels observed in the acoustic spectra of these fans, and particularly for large subharmonic humps. Excellent agreement between experimental and numerical noise predictions is finally reported stressing the dominant tip noise.
Springback Behavior of Aluminum/Polypropylene/Aluminum Sandwich Laminates
Authors: Kella Caroline K. , Mallick Pankaj K.
Published: November 2022

The springback of sheet metals after forming has been widely studied for decades using numerical and experimental methods. Many of these springback studies involve aluminum alloys. This study aims to understand the springback behavior of aluminum-polypropylene-aluminum laminates as they are being used increasingly in automotive and other applications because of their weight saving potential. A finite element model of the draw bending of a U-channel based on Numisheet’93 benchmark study is built using LS-DYNA. First, the model is validated and studied for springback prediction of single AA5182-O aluminum alloy sheets, and then it is extended to the study of the springback behaviors of AA5182-O/polypropylene/AA5182-O laminates with various combinations of core and skin thicknesses. The numerical model is also validated by experiment. Effects of various tool design and process parameters, such die radius, punch radius and blank holder force, on the springback of the sandwich laminates are studied. The effect of numerical modeling parameters is also considered.
Effect of Butt Gap on Stress Distribution and Carrying Capacity of X80 Pipeline Girth Weld
Authors: Zhu Lixia , Jia Haidong , Li Xiao , Luo Jinheng , Li Lifeng , Bai Dongdong
Published: November 2022

An unstable assembly gap is detrimental to the formation and performance of the pipeline butt girth weld joint. Therefore, a numerical model of an 18.4 mm-thick X80 pipeline girth weld by a homogeneous body heat source was established to investigate the effect of the butt gap on the joint temperature and stress field, and carrying capacity. The accuracy of the simulation results was verified by measuring the welding thermal cycle with a thermocouple. The investigation results showed that the weld pool, heat-affected zone (HAZ) width, and maximum circumferential stress of the joint rose with the increase in the butt gap. The tensile stress unfavorable to the joint quality was mainly distributed in the weld metal and partial HAZ, and the distribution areas gradually expanded as the gap increased. The Von Mises stress peak value of the joint appeared in the order of 3 mm > 2 mm > 1 mm > 0 mm gap, reaching the maximum of 467.3 MPa (3 mm gap). This variation trend is directly related to the improvement in welding heat input with increasing butt gaps. The maximum Von Mises stress of the joint was positively correlated with the carrying capacity of the pipeline, which diminished as the butt gap enlarged. The pipeline carrying capacity reached 17.8 MPa for the joint with no butt gap, and dropped to 13.1 MPa for the joint with a 3 mm gap. The relationship between the carrying capacity (P) and butt gap (C) was described by P = −0.125C2 − 1.135C + 17.715, through which the pipeline carrying capacity with other butt gaps can be predicted.
Dielectric and Spin-Glass Magnetic Properties of the A-Site Columnar-Ordered Quadruple Perovskite Sm2CuMn(MnTi3)O12
Authors: Belik Alexei A. , Liu Ran , Yamaura Kazunari
Published: November 2022

Perovskite-type ABO3 oxides show a number of cation-ordered structures, which have significant effects on their properties. The rock-salt-type order is dominant for B cations, and the layered order for A cations. In this work, we prepared a new perovskite-type oxide, Sm2CuMn(MnTi3)O12, with a rare columnar A-site order using a high-pressure, high-temperature method at about 6 GPa and about 1700 K. Its crystal structure was studied with synchrotron powder X-ray diffraction. The compound crystallizes in space group P42/nmc (No. 137) at room temperature with a = 7.53477 Å and c = 7.69788 Å. The magnetic properties of the compound were studied with dc and ac magnetic susceptibility measurements and specific heat. Spin-glass (SG) magnetic properties were found with TSG = 7 K, while specific heat, in the form of Cp/T, showed a strong, very broad anomaly developing below 20 K and peaking at 4 K. The dielectric constant of Sm2CuMn(MnTi3)O12 was nearly frequency and temperature independent between 8 K and 200 K, with a value of about 50. Cu2+ doping drastically modified the magnetic and dielectric properties of Sm2CuMn(MnTi3)O12 in comparison with the parent compound Sm2MnMn(MnTi3)O12, which showed a long-range ferrimagnetic order at 34–40 K. The antisite disorder of Cu2+ and Mn2+ cations between square-planar and octahedral sites was responsible for the SG magnetic properties of Sm2CuMn(MnTi3)O12.
Analysis of the Extrusion Process of Aluminium Alloy Profiles
Authors: Bajor Teresa , Kawałek Anna , Berski Szymon , Jurczak Henryk , Borowski Jacek
Published: November 2022

The paper presents an analysis of the results of numerical tests of the extrusion process of structural panels made of the 5xxx and 6xxx series aluminium alloys in a designed split die. The obtained products are intended for innovative superstructures of special car bodies. The main purpose of the research was the designed split die and numerical simulations and analysis of test results to determine the parameters of the extrusion process. The distribution of stress intensity, strain, strain rate, and temperature in the extruded metal was analysed for two different speeds of the punch movement. On the basis of the analysis of the distribution of stress values occurring in the extrusion process, the conditions enabling the real process of extrusion of the panel profile in industrial conditions in the designed split die were determined. It was shown that panel sections can be produced from ingots with a length of 770 mm on a press with a pressure of 35 MN (12”).
Chiral Dy(III) Fluorescent Single-Molecule Magnet Based on an Achiral Flexible Ligand
Authors: Zeng Min , Miao Lin , Wu Xue-Ru , Liu Cai-Ming , Kou Hui-Zhong
Published: November 2022

A novel multi-channel barcode module was developed by using chiral co-crystals which contain field-induced SMM behavior and different emission bands. The chiral co-crystals [Zn(H2L)Dy(DBM)2]4(ClO4)4⋅9CH3OH⋅H2O (1a) and [Zn(H2L)Dy(DBM)2]4(ClO4)4⋅8CH3OH⋅0.5H2O (1b) (H4L = 2,2′-[1,2-ethanediylbis[(hydroxyethylimino)methylene]]bis[6-methoxy-4-methyl-phenol], HDBM = dibenzoylmethane) were obtained through one-pot reaction of ZnII and DyIII with the achiral ligands H4L and HDBM. X-ray single crystal diffraction and CD spectroscopy confirmed that they are enantiomers crystallized in P43 (1a) and P41 (1b), both consisting of two ∆-[Zn(H2L)Dy(DBM)2]+ cations, two Λ-[Zn(H2L)Dy(DBM)2]+ cations and four (ClO4)− anions. The presence of DyIII ions endow them with the property of field-induced slow magnetic relaxation. The relatively low energy barrier of 35.0(9) K for complex 1 may be due to the poor axiality of the ligand field caused by the long Dy-Ophenoxy bond lengths and the small Ophenoxy-Dy-Ophenoxy bond angles. Moreover, when the organic ligands H4L (λex = 350 nm) and DyIII (λex = 420 nm) are excited, different emission spectra are observed.
A Simplified Approach to Pulmonary Vein Visualization during Cryoballoon Ablation of Atrial Fibrillation
Authors: Anwar Omar , Chung Da-Un , Gunawardene Melanie A. , Jungen Christiane , Hartmann Jens , Meyer Christian , Gessler Nele , Willems Stephan , Hakmi Samer , Eickholt Christian
Published: November 2022

Background and Objectives: Selective pulmonary vein (PV) angiography has been established as the gold standard for PV visualization in cryoballoon (CB)-based pulmonary vein isolation (PVI). We sought to simplify this approach to reduce procedural complexity and radiation exposure. Materials and Methods: Patients with paroxysmal and recently diagnosed persistent AF undergoing CB-based PVI from January 2015 to December 2017 were retrospectively analyzed. Patients underwent either selective PV angiography or conventional left atrial (LA) angiography for PV visualization. Results: A total of 336 patients were analyzed. A total of 87 patients (26%) received PV angiography and 249 (74%) LA angiography. LA angiography required fewer cine-sequences for PV visualization, translating into a significant reduction in procedure duration, fluoroscopy time and dose area product. Additionally, less contrast medium was utilized. PV occlusion by the CB, CB temperature and time to isolation showed no significant differences. The number of CB applications and total application time (LA angiography: 1.4 ± 0.02 vs. PV Angiography: 1.6 ± 0.05; p < 0.0001; LA angiography: 297.9 ± 4.62 vs. PV-Angiography: 348.9 ± 11.03; p < 0.001, respectively) per vein were slightly but significantly higher in the PV angiography group. We observed no difference in late AF recurrence (24.7% LA angiography vs. 21.3% PV angiography; p = 0.2657). Conclusions: A simplified protocol, using LA angiography for PV visualization, entails a reduction in procedure time and radiation exposure while equally maintaining procedural efficiency and safety in both groups.
Sex and Gender-Related Differences in the Outcome of Total Hip Arthroplasty: A Current Concepts Review
Authors: Solarino Giuseppe , Bizzoca Davide , Moretti Anna Maria , D’Apolito Rocco , Moretti Biagio , Zagra Luigi
Published: November 2022

Background and Objectives: Sex and gender-related differences may influence the outcome of patients undergoing total hip arthroplasty (THA). The present paper aims to depict the importance of sex and gender-related issues in the perioperative management of patients undergoing THA to improve clinical outcomes and prevent postoperative complications. Materials and Methods: From January 2002 to August 2022, OVID-MEDLINE, EMBASE, SCOPU S, Web of Science, Google Scholar, and PubMed were searched to identify relevant studies for further analysis. The search strategy included the following terms: ((“gender-related differences” [MeSH Terms] OR “sex-related differences” [All Fields]) OR (“gender indicators” [MeSH Terms] OR “sex” [All Fields])) AND (“total hip arthroplasty” [MeSH Terms] OR (total hip replacement [All Fields])). Results: Twenty-eight papers were included in this current concepts review. Sex and gender-related differences were analyzed with regard to the following points: (1) surgical approach, robotic surgery, scar cosmesis, and implant choice; (2) postoperative clinical outcome and complications; (3) sexual activity after THA; and (4) psychological status and daily functional requirements. The data analysis showed that female patients need more specific attention in the preoperative, intraoperative, and postoperative phases to improve clinical and functional outcomes, reduce complications risk, and manage patient satisfaction. Conclusions: THA outcomes may be influenced by sex and gender-related factors which should be carefully assessed and addressed in patients undergoing surgery to improve the postoperative outcomes of patients’ satisfaction and reduce postoperative complications that can differ between the two sexes.
How Telemedicine Can Improve the Quality of Care for Patients with Alzheimer’s Disease and Related Dementias? A Narrative Review
Authors: Angelopoulou Efthalia , Papachristou Nikolaos , Bougea Anastasia , Stanitsa Evangelia , Kontaxopoulou Dionysia , Fragkiadaki Stella , Pavlou Dimosthenis , Koros Christos , Değirmenci Yıldız , Papatriantafyllou John , Thireos Eleftherios , Politis Antonios , Tsouros Agis , Bamidis Panagiotis , Stefanis Leonidas , Papageorgiou Sokratis
Published: November 2022

Background and Objectives: Dementia affects more than 55 million patients worldwide, with a significant societal, economic, and psychological impact. However, many patients with Alzheimer’s disease (AD) and other related dementias have limited access to effective and individualized treatment. Care provision for dementia is often unequal, fragmented, and inefficient. The COVID-19 pandemic accelerated telemedicine use, which holds promising potential for addressing this important gap. In this narrative review, we aim to analyze and discuss how telemedicine can improve the quality of healthcare for AD and related dementias in a structured manner, based on the seven dimensions of healthcare quality defined by the World Health Organization (WHO), 2018: effectiveness, safety, people-centeredness, timeliness, equitability, integrated care, and efficiency. Materials and Methods: MEDLINE and Scopus databases were searched for peer-reviewed articles investigating the role of telemedicine in the quality of care for patients with dementia. A narrative synthesis was based on the seven WHO dimensions. Results: Most studies indicate that telemedicine is a valuable tool for AD and related dementias: it can improve effectiveness (better access to specialized care, accurate diagnosis, evidence-based treatment, avoidance of preventable hospitalizations), timeliness (reduction of waiting times and unnecessary transportation), patient-centeredness (personalized care for needs and values), safety (appropriate treatment, reduction of infection risk),integrated care (interdisciplinary approach through several dementia-related services), efficiency (mainly cost-effectiveness) and equitability (overcoming geographical barriers, cultural diversities). However, digital illiteracy, legal and organizational issues, as well as limited awareness, are significant potential barriers. Conclusions: Telemedicine may significantly improve all aspects of the quality of care for patients with dementia. However, future longitudinal studies with control groups including participants of a wide educational level spectrum will aid in our deeper understanding of the real impact of telemedicine in quality care for this population.