bluuebunny/crossref_metadata_2025 huggingface dataset:
Atypical Chronic Myeloid Leukemia: New Developments from Molecular Diagnosis to Treatment
Authors: Castellino Alessia , Santambrogio Elisa , Rapezzi Davide , Massaia Massimo
Published: October 2021

Atypical Chronic Myeloid Leukemia, BCR-ABL1 negative (aCML) is a rare hematological entity, included in the group of myelodysplastic (MDS)/myeloproliferative (MPN) overlap syndromes. It is characterized by an aggressive course, a high rate of acute myeloid leukemia (AML) transformation, and a dismal outcome. The clinical presentation includes splenomegaly and leukocytosis with neutrophilia and left-shifted granulocytosis accompanied by granulocytic dysplasia and sometimes multilineage dysplasia. In past years, the disease incidence was likely underestimated, as diagnosis was only based on morphological features. Recently, the improving knowledge in the molecular biology of MDS/MPN neoplasms has made it possible to distinguish aCML from other overlapping syndromes, basing on next generation sequencing. Among the most commonly mutated genes, several involve the Jak-STAT, MAPK, and ROCK signaling pathways, which could be actionable with targeted therapies that are already used in clinical practice, opening the way to tailored treatment in aCML. However, currently, there are few data available for small samples, and allogeneic transplant remains the only curative option for eligible patients.
The Role of miRNAs as Therapeutic Tools in Sickle Cell Disease
Authors: Cyrus Cyril
Published: October 2021

Background and Objectives: Sickle cell disorder (SCD) is a paradigmatic example of a complex monogenic disorder. SCD is characterized by the production of abnormal hemoglobin, primarily in the deoxygenated state, which makes erythrocytes susceptible to intracellular hemoglobin polymerization. Functional studies have affirmed that the dysregulation of miRNAs enhances clinical severity or has an ameliorating effect in SCD. miRNAs can be effectively regulated to reduce the pace of cell cycle progression, to reduce iron levels, to influence hemolysis and oxidative stress, and most importantly, to increase γ-globin gene expression and enhance the effectiveness of hydroxyurea. Results: This review highlights the roles played by some key miRNAs in hemoglobinopathies, especially in hematopoiesis, erythroid differentiation, and severity of anemia, which make miRNAs attractive molecular tools for innovative therapeutic approaches. Conclusions: In this era of targeted medicine, miRNAs mimics and antagomirs may be promising inducers of HbF synthesis which could ameliorate the clinical severity of SCD.
Efficient Photocatalytic Degradation of Organic Pollutant in Wastewater by Electrospun Functionally Modified Polyacrylonitrile Nanofibers Membrane Anchoring TiO2 Nanostructured
Authors: AlAbduljabbar Fahad A. , Haider Sajjad , Ali Fekri Abdulraqeb Ahmed , Alghyamah Abdulaziz A. , Almasry Waheed A. , Patel Raj , Mujtaba Iqbal M.
Published: October 2021

In this study, polyacrylonitrile (PAN_P) nanofibers (NFs) were fabricated by electrospinning. The PAN_P NFs membrane was functionalized with diethylenetriamine to prepare a functionalized polyacrylonitrile (PAN_F) NFs membrane. TiO2 nanoparticles (NPs) synthesized in the laboratory were anchored to the surface of the PAN_F NFs membrane by electrospray to prepare a TiO2 NPs coated NFs membrane (PAN_Coa). A second TiO2/PAN_P composite membrane (PAN_Co) was prepared by embedding TiO2 NPs into the PAN_P NFs by electrospinning. The membranes were characterized by microscopic, spectroscopic and X-ray techniques. Scanning electron micrographs (SEM) revealed smooth morphologies for PAN_P and PAN_F NFs membranes and a dense cloud of TiO2 NPs on the surface of PAN_Coa NFs membrane. The attenuated total reflectance in the infrared (ATR-IR) proved the addition of the new amine functionality to the chemical structure of PAN. Transmission electron microscope images (TEM) revealed spherical TiO2 NPs with sizes between 18 and 32 nm. X-ray powder diffraction (XRD) patterns and energy dispersive X-ray spectroscopy (EDX) confirmed the existence of the anatase phase of TiO2. Surface profilometry da-ta showed increased surface roughness for the PAN_F and PAN_Coa NFs membranes. The adsorption-desorption isotherms and hysteresis loops for all NFs membranes followed the IV -isotherm and the H3 -hysteresis loop, corresponding to mesoporous and slit pores, respectively. The photocatalytic activities of PAN_Coa and PAN_Co NFs membranes against methyl orange dye degradation were evaluated and compared with those of bare TiO2 NPs.The higher photocatalytic activity of PAN_Coa membrane (92%, 20 ppm) compared to (PAN_Co) NFs membrane (41.64%, 20 ppm) and bare TiO2 (49.60%, 20 ppm) was attributed to the synergy between adsorption, lower band gap, high surface roughness and surface area.
Simplified Model of a High Burnup Spent Nuclear Fuel Rod under Lateral Impact Considering a Stress-Based Failure Criterion
Authors: Kim Seyeon , Lee Sanghoon
Published: October 2021

The inventory of spent nuclear fuel (SNF) generated in nuclear power plants is continuously increasing, and it is very important to maintain the structural integrity of SNF for economical and efficient management. The cladding surrounding nuclear fuel must be protected from physical and mechanical deterioration, which causes fuel rod breakage. In this study, the material properties of the simplified beam model of a SNF rod were calibrated for a drop accident evaluation by considering the pellet–clad interaction (PCI) of the high burnup fuel rod. In a horizontal drop, which is the most damaging during a drop accident of SNF, the stress in the cladding caused by the inertia action of the pellets has a great effect on the integrity of the fuel rod. The failure criterion for SNF was selected as the membrane plus bending stress through stress linearization in the cross-sections through the thickness of the cladding. Because the stress concentration in the cladding around the vicinity of the pellet–pellet interface cannot be simulated in a simplified beam model, a stress correction factor is derived through a comparison of the simplified model and detailed model. The applicability of the developed simplified model is checked through dynamic impact simulations. The developed model can be used in cask level analyses and is expected to be usefully utilized to evaluate the structural integrity of SNF under transport and in storage conditions.
Influence of Cooling Rate on Microstructure Formation of Si–Mo Ductile Iron Castings
Authors: Górny Marcin , Kawalec Magdalena , Gracz Beata , Tupaj Mirosław
Published: October 2021

The present study highlights the effect of the cooling rate on the microstructure formation of Si–Mo ductile iron. In this study, experiments were carried out for castings with different wall thicknesses (i.e., 3, 5, 13, and 25 mm) to achieve various cooling rates. The simulation of the cooling and solidification was performed through MAGMASOFT to correlate the cooling conditions with the microstructure. The phase diagram of the investigated alloy was calculated using Thermo-Calc, whereas the quantitative metallography analyses using scanning electron microscopy and optical microscopy were performed to describe the graphite nodules and metallic matrix morphologies. The present study provides insights into the effect of the cooling rate on the graphite nodule count, nodularity, and volumetric fractions of graphite and ferrite as well as the average ferritic grain size of thin-walled and reference Si–Mo ductile iron castings. The study shows that the cooling rates of castings vary within a wide range (27 °C–1.5 °C/s) when considering wall thicknesses of 3 to 25 mm. The results also suggest that the occurrence of pearlite and carbides are related to segregations during solidification rather than to cooling rates at the eutectoid temperature. Finally, the present study shows that the longitudinal ultrasonic wave velocity is in linear dependence with the number of graphite nodules of EN-GJS-SiMo45-6 ductile iron.
Structural-Phase State, Mechanical Properties, Acoustic and Magnetic Characteristics in the Sustainable Deformation Localization Zones of Power Equipment Made of Structural and Heat Resistant Steels
Authors: Ababkov Nikolay , Smirnov Alexandr , Danilov Vladimir , Zuev Lev , Popova Natalya , Nikonenko Elena
Published: October 2021

The paper presents the results of the analysis of the microstructure, mechanical properties, acoustic and magnetic characteristics of the metal of pipelines that are part of heat and power equipment, after long-term operation, made of structural and heat-resistant steels in the zones of localization of plastic deformation. Samples of 0.2 C steel and 0.12C-1Cr-1Mo-1V steel were studied in the initial state, as well as after operation for 219 and 360 thousand hours, respectively. As a result of the studies carried out for each sample, the phase composition was determined (qualitatively and quantitatively), and the following parameters of the fine structure were calculated: volume fractions of structural components of steel (pearlite and ferrite), scalar ρ and excess ρ± dislocation density, curvature-torsion of the crystal lattice χ, amplitude of internal stresses (shear stress and long-range stresses). All quantitative parameters of the structure are determined both in each structural component of steel, and in general for each sample. The structure of the metal of all specimens after deformation before the formation of zones of stable localization of deformations consists of a ferrite-pearlite mixture, and for specimens after operation before fracture only of unfragmented and fragmented ferrite. Ferrite, which occupies the bulk of the material, is present both unfragmented and fragmented. For all samples, the ratios ρ ≥ ρ±, χ = χpl, σL ≥ σd were calculated, which indicate whether there is a danger of the initiation of microcracks in metal samples. For specimens without operation and after operation without damage in zones of stable localization of deformations, these conditions are met, and for specimens after operation until destruction, they are not met. It was found that the structural-phase state in the zones of localization of deformations has a direct effect on the characteristics of non-destructive tests. Thus, for all investigated samples, the values of such parameters as the delay time of the surface acoustic wave, the attenuation coefficient, the amplitude of the received signal, and the intensity of magnetic noise in the zones of deformation localization were established.
Application of Thin-Film Microextraction to Analyze Volatile Metabolites in A549 Cancer Cells
Authors: Filipiak Wojciech , Jaroch Karol , Szeliska Paulina , Żuchowska Karolina , Bojko Barbara
Published: October 2021

Volatile organic compounds (VOCs) have been proposed in the last two decades as biomarkers for disease detection and therapeutic monitoring. Model in vitro experiments with established cell lines are fundamental to clarify whether given VOCs originate from normal human cells or pathogens, including transformed cancer cells. Due to the trace concentrations of target metabolites, adsorptive enrichment is needed before gas chromatography-mass spectrometry (GC-MS) analysis, with solid-phase microextraction (SPME) being perfectly suited for this purpose. Here, a modification of SPME, the thin-film microextraction (TFME) technique, is proposed for analysis of cellular VOCs, which utilizes a planar mesh coated with stationary phase to increase the extraction phase volume and active surface area. In this study, four different adsorbents were compared: carboxen, divinylbenzene, hydrophobic−lipophilic balanced and polydimethylsiloxane. Amongst them, HLB sheets using poly(divinylbenzene-co-N-vinyl-pyrrolidone) skeleton structure proved to be the most versatile, enabling the most sensitive analysis of VOCs with a broad polarity and volatility. For HLB, sampling type (internal static headspace, external bi-directional headspace), extraction temperature and extraction time were also examined. An established method was successfully applied to analyze metabolites produced by A549 cells revealing five volatiles at significantly higher (additionally benzaldehyde at lower) levels in cell culture medium compared to the cell-free reference medium headspace.
Multi-Input Logic-in-Memory for Ultra-Low Power Non-Von Neumann Computing
Authors: Zanotti Tommaso , Pavan Paolo , Puglisi Francesco Maria
Published: October 2021

Logic-in-memory (LIM) circuits based on the material implication logic (IMPLY) and resistive random access memory (RRAM) technologies are a candidate solution for the development of ultra-low power non-von Neumann computing architectures. Such architectures could enable the energy-efficient implementation of hardware accelerators for novel edge computing paradigms such as binarized neural networks (BNNs) which rely on the execution of logic operations. In this work, we present the multi-input IMPLY operation implemented on a recently developed smart IMPLY architecture, SIMPLY, which improves the circuit reliability, reduces energy consumption, and breaks the strict design trade-offs of conventional architectures. We show that the generalization of the typical logic schemes used in LIM circuits to multi-input operations strongly reduces the execution time of complex functions needed for BNNs inference tasks (e.g., the 1-bit Full Addition, XNOR, Popcount). The performance of four different RRAM technologies is compared using circuit simulations leveraging a physics-based RRAM compact model. The proposed solution approaches the performance of its CMOS equivalent while bypassing the von Neumann bottleneck, which gives a huge improvement in bit error rate (by a factor of at least 108) and energy-delay product (projected up to a factor of 1010).
A Novel Step–Doped Channel AlGaN/GaN HEMTs with Improved Breakdown Performance
Authors: Liu Jianhua , Guo Yufeng , Zhang Jun , Yao Jiafei , Li Man , Zhang Maolin , Chen Jing , Huang Xiaoming , Huang Chenyang
Published: October 2021

The AlGaN/GaN high electron mobility transistor with a step–doped channel (SDC–HEMT) is first proposed in this paper. The potential distribution and the electric field (E–field) distribution of the device are explored by the numerical approach and analytical approach simultaneously. By introducing extra dopants to the channel layer, the E–field distribution along the AlGaN/GaN heterojunction interface is reshaped, resulting in an improved breakdown characteristic. An optimized doping concentration gradient of channel layer of 2 × 1016 cm−3/step is proposed and verified by simulations. The breakdown voltage (BV) of the optimized SDC–HEMT reaches 1486 V with a 59.8% improvement compared with conventional AlGaN/GaN HEMT. In addition, the average E–field in the region between gate and drain improves from 1.5 to 2.5 MV/cm. Based on the equivalent potential method (EPM), an analytical model of the E–field and potential distribution is presented. The veracity and effectiveness of the proposed methodology is verified by the good agreement between the simulated and modeled results.
Systematic Review: Microfluidics and Plasmodium
Authors: Thorne Nicolas , Flores-Olazo Luis , Egoávil-Espejo Rocío , Vela Emir A. , Noel Julien , Valdivia-Silva Julio , van Noort Danny
Published: October 2021

Malaria affects 228 million people worldwide each year, causing severe disease and worsening the conditions of already vulnerable populations. In this review, we explore how malaria has been detected in the past and how it can be detected in the future. Our primary focus is on finding new directions for low-cost diagnostic methods that unspecialized personnel can apply in situ. Through this review, we show that microfluidic devices can help pre-concentrate samples of blood infected with malaria to facilitate the diagnosis. Importantly, these devices can be made cheaply and be readily deployed in remote locations.
Investigations on the Degradation of the Bile Salt Cholate via the 9,10-Seco-Pathway Reveals the Formation of a Novel Recalcitrant Steroid Compound by a Side Reaction in Sphingobium sp. Strain Chol11
Authors: Feller Franziska Maria , Eilebrecht Sebastian , Nedielkov Ruslan , Yücel Onur , Alvincz Julia , Salinas Gabriela , Ludwig Kevin Christopher , Möller Heiko , Philipp Bodo
Published: October 2021

Bile salts such as cholate are steroid compounds from the digestive tracts of vertebrates, which enter the environment upon excretion, e.g., in manure. Environmental bacteria degrade bile salts aerobically via two pathway variants involving intermediates with Δ1,4- or Δ4,6-3-keto-structures of the steroid skeleton. Recent studies indicated that degradation of bile salts via Δ4,6-3-keto intermediates in Sphingobium sp. strain Chol11 proceeds via 9,10-seco cleavage of the steroid skeleton. For further elucidation, the presumptive product of this cleavage, 3,12β-dihydroxy-9,10-seco-androsta-1,3,5(10),6-tetraene-9,17-dione (DHSATD), was provided to strain Chol11 in a co-culture approach with Pseudomonas stutzeri Chol1 and as purified substrate. Strain Chol11 converted DHSATD to the so far unknown compound 4-methyl-3-deoxy-1,9,12-trihydroxyestra-1,3,5(10)7-tetraene-6,17-dione (MDTETD), presumably in a side reaction involving an unusual ring closure. MDTETD was neither degraded by strains Chol1 and Chol11 nor in enrichment cultures. Functional transcriptome profiling of zebrafish embryos after exposure to MDTETD identified a significant overrepresentation of genes linked to hormone responses. In both pathway variants, steroid degradation intermediates transiently accumulate in supernatants of laboratory cultures. Soil slurry experiments indicated that bacteria using both pathway variants were active and also released their respective intermediates into the environment. This instance could enable the formation of recalcitrant steroid metabolites by interspecies cross-feeding in agricultural soils.
In Vitro Synergism of Penicillin and Ceftriaxone against Enterococcus faecalis
Authors: Thieme Lara , Briggs Simon , Duffy Eamon , Makarewicz Oliwia , Pletz Mathias W.
Published: October 2021

Enterococcus faecalis infective endocarditis is commonly treated with intravenous ampicillin/ceftriaxone combination therapy. Ampicillin, however, is unsuitable for outpatient parenteral antibiotic therapy (OPAT) regimens due to its instability in 24 h continuous infusors, and has been successfully replaced by benzylpenicillin used together with ceftriaxone in a few small case series. Since in vitro synergy data of penicillin/ceftriaxone against E. faecalis are still lacking, checkerboard assays were performed for 28 clinical E. faecalis isolates and one laboratory standard strain. Synergistic effects (both lowest and median FICI) were observed for penicillin/ceftriaxone in 15/29 isolates, while ampicillin/ceftriaxone exhibited synergism in 22/29 isolates. For isolates with ceftriaxone MICs ≤ 256 mg/L, the addition of free ceftriaxone trough concentrations to penicillin or ampicillin resulted in comparable synergistic effects for both combinations. In contrast, for isolates with ceftriaxone MICs ≥ 512 mg/L free ceftriaxone trough concentrations were only sufficient to exhibit synergistic effects in combination with ampicillin, but not penicillin. This study suggests that benzylpenicillin/ceftriaxone would be expected to be suitable for the OPAT treatment of enterococcal endocarditis for E. faecalis isolates with ceftriaxone MICs ≤ 256 mg/L. However, combination therapy would be expected to provide no advantage over benzylpenicillin monotherapy for isolates with ceftriaxone MICs ≥ 512 mg/L. Further investigation is required to analyse the relationship between ceftriaxone susceptibility and penicillin/ceftriaxone synergy, especially for isolates with ceftriaxone MICs of 64 to 512 mg/L.
DCMC as a Promising Alternative to Bentonite in White Wine Stabilization. Impact on Protein Stability and Wine Aromatic Fraction
Authors: Saracino Francesco , Brinco João , Gago Diana , Gomes da Silva Marco , Boavida Ferreira Ricardo , Ricardo-da-Silva Jorge , Chagas Ricardo , Ferreira Luísa Maria
Published: October 2021

Protein haze in white wine is one of the most common non-microbial defects of commercial wines, with bentonite being the main solution utilized by the winemaking industry to tackle this problem. Bentonite presents some serious disadvantages, and several alternatives have been proposed. Here, an alternative based on a new cellulose derivative (dicarboxymethyl cellulose, DCMC) is proposed. To determine the efficiency of DCMC as a bentonite alternative, three monovarietal wines were characterized, and their protein instability and content determined by a heat stability test (HST) and the Bradford method, respectively. The wines were treated with DCMC to achieve stable wines, as shown by the HST, and the efficacy of the treatments was assessed by determining, before and after treatment, the wine content in protein, phenolic compounds, sodium, calcium, and volatile organic compounds (VOCs) as well as the wine pH. DCMC applied at dosages such as those commonly employed for bentonite was able to reduce the protein content in all tested wines and to stabilize all but the Moscatel de Setúbal varietal wine. In general, DCMC was shown to induce lower changes in the wine pH and phenolic content than bentonite, reducing the wine calcium content. Regarding which VOCs are concerned, DCMC produced a general impact similar to that of bentonite, with differences depending on wine variety. The results obtained suggest that DCMC can be a sustainable alternative to bentonite in protein white wine stabilization.
The Influence of the Preparation Method on the Physico-Chemical Properties and Catalytic Activities of Ce-Modified LDH Structures Used as Catalysts in Condensation Reactions
Authors: Stamate Alexandra-Elisabeta , Zăvoianu Rodica , Pavel Octavian Dumitru , Birjega Ruxandra , Matei Andreea , Dumitru Marius , Brezeștean Ioana , Osiac Mariana , Marcu Ioan-Cezar
Published: October 2021

Mechanical activation and mechanochemical reactions are the subjects of mechanochemistry, a special branch of chemistry studied intensively since the 19th century. Herein, we comparably describe two synthesis methods used to obtain the following layered double hydroxide doped with cerium, Mg3Al0.75Ce0.25(OH)8(CO3)0.5·2H2O: the mechanochemical route and the co-precipitation method, respectively. The influence of the preparation method on the physico-chemical properties as determined by multiple techniques such as XRD, SEM, EDS, XPS, DRIFT, RAMAN, DR-UV-VIS, basicity, acidity, real/bulk densities, and BET measurements was also analyzed. The obtained samples, abbreviated HTCe-PP (prepared by co-precipitation) and HTCe-MC (prepared by mechanochemical method), and their corresponding mixed oxides, Ce-PP (resulting from HTCe-PP) and Ce-MC (resulting from HTCe-MC), were used as base catalysts in the self-condensation reaction of cyclohexanone and two Claisen–Schmidt condensations, which involve the reaction between an aromatic aldehyde and a ketone, at different molar ratios to synthesize compounds with significant biologic activity from the flavonoid family, namely chalcone (1,3-diphenyl-2-propen-1-one) and flavone (2-phenyl-4H-1benzoxiran-4-one). The mechanochemical route was shown to have indisputable advantages over the co-precipitation method for both the catalytic activity of the solids and the costs.
Effect of Crosslinkers on Optical and Mechanical Behavior of Chiral Nematic Liquid Crystal Elastomers
Authors: Ku Kyosun , Hisano Kyohei , Yuasa Kyoko , Shigeyama Tomoki , Akamatsu Norihisa , Shishido Atsushi , Tsutsumi Osamu
Published: October 2021

Chiral nematic (N*) liquid crystal elastomers (LCEs) are suitable for fabricating stimuli-responsive materials. As crosslinkers considerably affect the N*LCE network, we investigated the effects of crosslinking units on the physical properties of N*LCEs. The N*LCEs were synthesized with different types of crosslinkers, and the relationship between the N*LC polymeric system and the crosslinking unit was investigated. The N*LCEs emit color by selective reflection, in which the color changes in response to mechanical deformation. The LC-type crosslinker decreases the helical twisting power of the N*LCE by increasing the total molar ratio of the mesogenic compound. The N*LCE exhibits mechano-responsive color changes by coupling the N*LC orientation and the polymer network, where the N*LCEs exhibit different degrees of pitch variation depending on the crosslinker. Moreover, the LC-type crosslinker increases the Young’s modulus of N*LCEs, and the long methylene chains increase the breaking strain. An analysis of experimental results verified the effect of the crosslinkers, providing a design rationale for N*LCE materials in mechano-optical sensor applications.
In Silico Identification and Validation of Organic Triazole Based Ligands as Potential Inhibitory Drug Compounds of SARS-CoV-2 Main Protease
Authors: Sur Vishma Pratap , Sen Madhab Kumar , Komrskova Katerina
Published: October 2021

The SARS-CoV-2 virus is highly contagious to humans and has caused a pandemic of global proportions. Despite worldwide research efforts, efficient targeted therapies against the virus are still lacking. With the ready availability of the macromolecular structures of coronavirus and its known variants, the search for anti-SARS-CoV-2 therapeutics through in silico analysis has become a highly promising field of research. In this study, we investigate the inhibiting potentialities of triazole-based compounds against the SARS-CoV-2 main protease (Mpro). The SARS-CoV-2 main protease (Mpro) is known to play a prominent role in the processing of polyproteins that are translated from the viral RNA. Compounds were pre-screened from 171 candidates (collected from the DrugBank database). The results showed that four candidates (Bemcentinib, Bisoctrizole, PYIITM, and NIPFC) had high binding affinity values and had the potential to interrupt the main protease (Mpro) activities of the SARS-CoV-2 virus. The pharmacokinetic parameters of these candidates were assessed and through molecular dynamic (MD) simulation their stability, interaction, and conformation were analyzed. In summary, this study identified the most suitable compounds for targeting Mpro, and we recommend using these compounds as potential drug molecules against SARS-CoV-2 after follow up studies.
Biohydrometallurgy for Rare Earth Elements Recovery from Industrial Wastes
Authors: Castro Laura , Blázquez María Luisa , González Felisa , Muñoz Jesús Ángel
Published: October 2021

Biohydrometallurgy recovers metals through microbially mediated processes and has been traditionally applied for the extraction of base metals from low-grade sulfidic ores. New investigations explore its potential for other types of critical resources, such as rare earth elements. In recent times, the interest in rare earth elements (REEs) is growing due to of their applications in novel technologies and green economy. The use of biohydrometallurgy for extracting resources from waste streams is also gaining attention to support innovative mining and promote a circular economy. The increase in wastes containing REEs turns them into a valuable alternative source. Most REE ores and industrial residues do not contain sulfides, and bioleaching processes use autotrophic or heterotrophic microorganisms to generate acids that dissolve the metals. This review gathers information towards the recycling of REE-bearing wastes (fluorescent lamp powder, spent cracking catalysts, e-wastes, etc.) using a more sustainable and environmentally friendly technology that reduces the impact on the environment.
Reconstitution of Caveolin-1 into Artificial Lipid Membrane: Characterization by Transmission Electron Microscopy and Solid-State Nuclear Magnetic Resonance
Authors: Zhang Yanli , Zhang Xinyan , Kong Wenru , Wang Shuqi
Published: October 2021

Caveolin-1 (CAV1), a membrane protein that is necessary for the formation and maintenance of caveolae, is a promising drug target for the therapy of various diseases, such as cancer, diabetes, and liver fibrosis. The biology and pathology of caveolae have been widely investigated; however, very little information about the structural features of full-length CAV1 is available, as well as its biophysical role in reshaping the cellular membrane. Here, we established a method, with high reliability and reproducibility, for the expression and purification of CAV1. Amyloid-like properties of CAV1 and its C-terminal peptide CAV1(168-178) suggest a structural basis for the short linear CAV1 assemblies that have been recently observed in caveolin polyhedral cages in Escherichia coli (E. coli). Reconstitution of CAV1 into artificial lipid membranes induces a caveolae-like membrane curvature. Structural characterization of CAV1 in the membrane by solid-state nuclear magnetic resonance (ssNMR) indicate that it is largely α-helical, with very little β-sheet content. Its scaffolding domain adopts a α-helical structure as identified by chemical shift analysis of threonine (Thr). Taken together, an in vitro model was developed for the CAV1 structural study, which will further provide meaningful evidences for the design and screening of bioactive compounds targeting CAV1.
Characterization, Antioxidant and Anti-Inflammation Capacities of Fermented Flammulina velutipes Polyphenols
Authors: Ma Sheng , Zhang Hongcai , Xu Jianxiong
Published: October 2021

This work investigated the preparation, characterization, antioxidant, and anti-inflammation capacities of Flammulina velutipes polyphenols (FVP) and fermented FVP (FFVP). The results revealed that the new syringic acid, accounting for 22.22%, was obtained after fermentation (FFVP). FFVP exhibits higher antioxidant and anti-inflammation activities than FVP, enhancing cell viability and phagocytosis, inhibiting the secretion of NO and ROS, and reducing the inflammatory response of RAW264.7 cells. This study revealed that FFVP provides a theoretical reference for in-depth study of its regulatory mechanisms and further development of functional antioxidants that are applicable in the food and health industry.
Androstenedione (a Natural Steroid and a Drug Supplement): A Comprehensive Review of Its Consumption, Metabolism, Health Effects, and Toxicity with Sex Differences
Authors: Badawy Marwa T. , Sobeh Mansour , Xiao Jianbo , Farag Mohamed A.
Published: October 2021

Androstenedione is a steroidal hormone produced in male and female gonads, as well as in the adrenal glands, and it is known for its key role in the production of estrogen and testosterone. Androstenedione is also sold as an oral supplement, that is being utilized to increase testosterone levels. Simply known as “andro” by athletes, it is commonly touted as a natural alternative to anabolic steroids. By boosting testosterone levels, it is thought to be an enhancer for athletic performance, build body muscles, reduce fats, increase energy, maintain healthy RBCs, and increase sexual performance. Nevertheless, several of these effects are not yet scientifically proven. Though commonly used as a supplement for body building, it is listed among performance-enhancing drugs (PEDs) which is banned by the World Anti-Doping Agency, as well as the International Olympic Committee. This review focuses on the action mechanism behind androstenedione’s health effects, and further side effects including clinical features, populations at risk, pharmacokinetics, metabolism, and toxicokinetics. A review of androstenedione regulation in drug doping is also presented.
Role of Na-Montmorillonite on Microbially Induced Calcium Carbonate Precipitation
Authors: Tang Guowang , Jia Cangqin , Wang Guihe , Yu Peizhi , Zhang Haonan
Published: October 2021

The use of additives has generated significant attention due to their extensive application in the microbially induced calcium carbonate precipitation (MICP) process. This study aims to discuss the effects of Na-montmorillonite (Na-MMT) on CaCO3 crystallization and sandy soil consolidation through the MICP process. Compared with the traditional MICP method, a larger amount of CaCO3 precipitate was obtained. Moreover, the reaction of Ca2+ ions was accelerated, and bacteria were absorbed by a small amount of Na-MMT. Meanwhile, an increase in the total cementing solution (TCS) was not conducive to the previous reaction. This problem was solved by conducting the reaction with Na-MMT. The polymorphs and morphologies of the CaCO3 precipitates were tested by using X-ray diffraction and scanning electron microscopy. Further, when Na-MMT was used, the morphology of CaCO3 changed from an individual precipitate to agglomerations of the precipitate. Compared to the experiments without Na-MMT in the MICP process, the addition of Na-MMT significantly reduced the hydraulic conductivity (HC) of sandy soil consolidated.
One-Pot Synthesis of Nitrogen-Doped TiO2 with Supported Copper Nanocrystalline for Photocatalytic Environment Purification under Household White LED Lamp
Authors: Pan Yixiao , Wang Yifei , Wu Shimiao , Chen Yating , Zheng Xiangrong , Zhang Ning
Published: October 2021

Developing efficient and cheap photocatalysts that are sensitive to indoor light is promising for the practical application of photocatalysis technology. Here, N-doped TiO2 photocatalyst with loaded Cu crystalline cocatalyst is synthesized by a simple one-pot method. The structure is confirmed by transmission electron microscopy and X-ray photoelectron spectroscopy analysis, which exhibit that Cu metal nanocrystalline is uniformly deposited on the surface of N-doped TiO2 material. UV-Vis absorption spectra illustrate that the modified samples possess favorable visible light absorption properties and suppressed-electron hole separation. The as-fabricated Cu-loaded N-TiO2 materials show high activity in photocatalytic decomposing isopropanol and inactivating E. coli under the irradiation of a household white LED lamp. The developed synthetic strategy and photocatalytic materials reported here are promising for indoor environment purification.
Influence of Ce, Nd, Eu and Tm Dopants on the Properties of InSe Monolayer: A First-Principles Study
Authors: Xie Zhi , Chen Limin
Published: October 2021

Doping of foreign atoms may substantially alter the properties of the host materials, in particular low-dimension materials, leading to many potential functional applications. Here, we perform density functional theory calculations of two-dimensional InSe materials with substitutional doping of lanthanide atoms (Ce, Nd, Eu, Tm) and investigate systematically their structural, magnetic, electronic and optical properties. The calculated formation energy shows that the substitutional doping of these lanthanide atoms is feasible in the InSe monolayer, and such doping is more favorable under Se-rich than In-rich conditions. As for the structure, doping of lanthanide atoms induces visible outward movement of the lanthanide atom and its surrounding Se atoms. The calculated total magnetic moments are 0.973, 2.948, 7.528 and 1.945 μB for the Ce-, Nd-, Eu-, and Tm-doped systems, respectively, which are mainly derived from lanthanide atoms. Further band structure calculations reveal that the Ce-doped InSe monolayer has n-type conductivity, while the Nd-doped InSe monolayer has p-type conductivity. The Eu- and Tm-doped systems are found to be diluted magnetic semiconductors. The calculated optical response of absorption in the four doping cases shows redshift to lower energy within the infrared range compared with the host InSe monolayer. These findings suggest that doping of lanthanide atoms may open up a new way of manipulating functionalities of InSe materials for low-dimension optoelectronics and spintronics applications.
Physical–Chemical Exfoliation of n-Alkylamine Derivatives of Layered Perovskite-like Oxide H2K0.5Bi2.5Ti4O13 into Nanosheets
Authors: Minich Iana A. , Silyukov Oleg I. , Kurnosenko Sergei A. , Gak Veronika V. , Kalganov Vladimir D. , Kolonitskiy Petr D. , Zvereva Irina A.
Published: October 2021

In the present work, we report the results on exfoliation and coating formation of inorganic–organic hybrids based on the layered perovskite-like bismuth titanate H2K0.5Bi2.5Ti4O13·H2O that could be prepared by a simple ion exchange reaction from a Ruddlesden–Popper phase K2.5Bi2.5Ti4O13. The inorganic–organic hybrids were synthesized by intercalation reactions. Exfoliation into nanosheets was performed for the starting hydrated protonated titanate and for the derivatives intercalated by n-alkylamines to study the influence of preliminary intercalation on exfoliation efficiency. The selected precursors were exfoliated in aqueous solutions of tetrabutylammonium hydroxide using facile stirring and ultrasonication. The suspensions of nanosheets obtained were characterized using UV–vis spectrophotometry, dynamic light scattering, inductively coupled plasma spectroscopy, and gravimetry. Nanosheets were coated on preliminarily polyethyleneimine-covered Si substrates using a self-assembly procedure and studied using atomic force and scanning electron microscopy.
Plasma-Driven Photocatalysis Based on Gold Nanoporous Arrays
Authors: Zhang Lisheng , Zhang Yiyuan , Wang Xueyan , Zhang Duan
Published: October 2021

Various effects caused by surface plasmons including enhanced electromagnetic field, local heating, and excited electrons/holes can not only redistribute the electromagnetic field in the time domain and space but also redistribute the excited carriers and drive chemical reactions. In this study, firstly, an Au nanoporous array photocatalyst with the arrayed gauge was prepared by means of the anodic alumina template. Then, the formation of 4,4′-dimercaptoazobenzene (DMAB) by the surface plasmon-driven photocatalysis under 633 nm laser irradiation was investigated by means of Raman spectroscopy using aminothiophenol (PATP) as a probe molecule on gold nananoporous arrays. In addition, sodium borohydride was introduced in situ to realize the reverse photocatalytic reaction driven by the surface plasma. With the help of FDTD software, the plasma distribution characteristics on the surface of Au nanoporous arrays were simulated and analyzed. Through this practical method, it is expected to draw specific graphics, letters, and Chinese characters on the micro/nano scale, and realize the functions of graphics drawing, information encryption, reading, and erasing on the micro/nano scale.
Quantization of Gravity and Finite Temperature Effects
Authors: Park I. Y.
Published: October 2021

Gravity is perturbatively renormalizable for the physical states which can be conveniently defined via foliation-based quantization. In recent sequels, one-loop analysis was explicitly carried out for Einstein-scalar and Einstein-Maxwell systems. Various germane issues and all-loop renormalizability have been addressed. In the present work we make further progress by carrying out several additional tasks. Firstly, we present an alternative 4D-covariant derivation of the physical state condition by examining gauge choice-independence of a scattering amplitude. To this end, a careful dichotomy between the ordinary, and large gauge symmetries is required and appropriate gauge-fixing of the ordinary symmetry must be performed. Secondly, vacuum energy is analyzed in a finite-temperature setup. A variant optimal perturbation theory is implemented to two-loop. The renormalized mass determined by the optimal perturbation theory turns out to be on the order of the temperature, allowing one to avoid the cosmological constant problem. The third task that we take up is examination of the possibility of asymptotic freedom in finite-temperature quantum electrodynamics. In spite of the debates in the literature, the idea remains reasonable.
Bacteria–Cancer Interface: Awaiting the Perfect Storm
Authors: Hansen Jonathan , Ali Waled , Sivadasan Rajeeve , Rajeeve Karthika
Published: October 2021

Epidemiological evidence reveal a very close association of malignancies with chronic inflammation as a result of persistent bacterial infection. Recently, more studies have provided experimental evidence for an etiological role of bacterial factors disposing infected tissue towards carcinoma. When healthy cells accumulate genomic insults resulting in DNA damage, they may sustain proliferative signalling, resist apoptotic signals, evade growth suppressors, enable replicative immortality, and induce angiogenesis, thus boosting active invasion and metastasis. Moreover, these cells must be able to deregulate cellular energetics and have the ability to evade immune destruction. How bacterial infection leads to mutations and enriches a tumour-promoting inflammatory response or micro-environment is still not clear. In this review we showcase well-studied bacteria and their virulence factors that are tightly associated with carcinoma and the various mechanisms and pathways that could have carcinogenic properties.
Concern for Dirofilaria immitis and Macrocyclic Lactone Loss of Efficacy: Current Situation in the USA and Europe, and Future Scenarios
Authors: Diakou Anastasia , Prichard Roger K.
Published: October 2021

Dirofilaria immitis infection is one of the most severe parasitic diseases in dogs. Prevention is achieved by the administration of drugs containing macrocyclic lactones (MLs). These products are very safe and highly effective, targeting the third and fourth larval stages (L3, L4) of the parasite. Until 2011, claims of the ineffectiveness of MLs, reported as “loss of efficacy” (LOE), were generally attributed to owners’ non-compliance, or other reasons associated with inadequate preventative coverage. There was solid argumentation that a resistance problem is not likely to occur because of (i) the great extent of refugia, (ii) the complexity of resistance development to MLs, and (iii) the possible large number of genes involved in resistance selection. Nevertheless, today, it is unequivocally proven that ML-resistant D. immitis strains exist, at least in the Lower Mississippi region, USA. Accordingly, tools have been developed to evaluate and confirm the susceptibility status of D. immitis strains. A simple, in-clinic, microfilariae suppression test, 14-28 days after ML administration, and a “decision tree” (algorithm), including compliance and preventatives’ purchase history, and testing gaps, may be applied for assessing any resistant nature of the parasite. On the molecular level, specific SNPs may be used as markers of ML resistance, offering a basis for the validation of clinically suspected resistant strains. In Europe, no LOE/resistance claims have been reported so far, and the existing conditions (stray dogs, rich wildlife, majority of owned dogs not on preventive ML treatment) do not favor selection pressure on the parasites. Considering the genetic basis of resistance and the epizootiological characteristics of D. immitis, ML resistance neither establishes easily nor spreads quickly, a fact confirmed by the current known dispersion of the problem, which is limited. Nevertheless, ML resistance may propagate from an initial geographical point, via animal and vector mobility, to other regions, while it can also emerge as an independent evolutionary process in a new area. For these reasons, and considering the current chemoprophylaxis recommendations and increasing use of ML endectoparasiticides as a potential selection pressure, it is important to remain vigilant for the timely detection of any ML LOE/resistance, in all continents where D. immitis is enzootic.
Morphological Characteristics of Alveolar and Cystic Echinococcosis Lesions in Human Liver and Bone
Authors: Barth Thomas F. E. , Casulli Adriano
Published: October 2021

Among echinococcoses diseases of human interest, two have a global public health impact: cystic and alveolar echinococcosis caused by Echinococcus granulosus sensu lato and Echinococcus multilocularis, respectively. Cystic and alveolar echinococcosis are neglected infectious diseases epidemiologically and are clinically vastly different with distinct microscopic features. Because of the rareness of these zoonotic diseases, pathologists have limited diagnostic experience in the analysis of the lesions caused by Echinococcus tapeworms. Here, we describe the main microscopic features to be considered to characterize these lesions: laminated layer, central necrosis, growth pattern, and delineation from adjacent tissue. Moreover, immunohistology using monoclonal antibodies is of great diagnostic help in reaching a definitive diagnosis by identifying the laminated body and small particles of E. multilocularis (spems) and small particles of E. granulosus (spegs).
Interferometric Technique for the Spectral Characterization of High Frequency Current-Modulated Mid-Infrared Semiconductor Lasers
Authors: Bahr Marc-Simon , Wolff Marcus
Published: October 2021

We describe two methods, based on Michelson interferometery, that enable the determination of the absolute wavelength of current-modulated semiconductor lasers. By non-linear regression of the instantaneous frequency of the interference signal, the rate of change of the wavelength of the radiation can be inferred. Alternatively, the absolute wavelength can be directly calculated from the maxima and minima of the interference signal. In both cases a reference absorption line enables the determination of the absolute wavelength. Both methods offer respective advantages. The methods allow a highly resolved wavelength measurement under lower kilohertz range current-modulation with relatively little effort. As a result, we present the rates of wavelength change and absolute wavelengths exemplarily for a specific interband cascade laser. It is furthermore shown that the spectral dynamic range of the laser decreases with increasing modulation frequency.
A Computational Study of Molecular Mechanism of Chloroquine Resistance by Chloroquine Resistance Transporter Protein of Plasmodium falciparum via Molecular Modeling and Molecular Simulations
Authors: Patel Chandan , Roy Dipankar
Published: October 2021

The molecular mechanism of chloroquine resistance by the chloroquine resistance transporter protein of Plasmodium sp. is explored using molecular modeling and computational methods. The key mutation, lysine(K)-76 to threonine(T) (LYS76THR) in the transporter protein pertains to increased recognition of the protonated forms of the antimalarial drug. Such enhanced affinity can promote drug efflux from host digestive vacuole, rendering aminoquinoline-based treatment ineffective.
Calcium Sensor SlCBL4 Associates with SlCIPK24 Protein Kinase and Mediates Salt Tolerance in Solanum lycopersicum
Authors: Cho Joo Hyuk , Sim Sung-Chur , Kim Kyung-Nam
Published: October 2021

Soil salinity is one of the major environmental stresses that restrict the growth and development of tomato (Solanum lycopersicum L.) worldwide. In Arabidopsis, the calcium signaling pathway mediated by calcineurin B-like protein 4 (CBL4) and CBL-interacting protein kinase 24 (CIPK24) plays a critical role in salt stress response. In this study, we identified and isolated two tomato genes similar to the Arabidopsis genes, designated as SlCBL4 and SlCIPK24, respectively. Bimolecular fluorescence complementation (BiFC) and pull-down assays indicated that SlCBL4 can physically interact with SlCIPK24 at the plasma membrane of plant cells in a Ca2+-dependent manner. Overexpression of SlCBL4 or superactive SlCIPK24 mutant (SlCIPK24M) conferred salt tolerance to transgenic tomato (cv. Moneymaker) plants. In particular, the SlCIPK24M-overexpression lines displayed dramatically enhanced tolerance to high salinity. It is notable that the transgenic plants retained higher contents of Na+ and K+ in the roots compared to the wild-type tomato under salt stress. Taken together, our findings clearly suggest that SlCBL4 and SlCIPK24 are functional orthologs of the Arabidopsis counterpart genes, which can be used or engineered to produce salt-tolerant tomato plants.