A WHO 2021‐based comprehensive scheme outlining sperm parameters’ associations with IVF outcomes in PGT‐A cycles
Authors: Mazzilli Rossella , Cimadomo Danilo , Innocenti Federica , Taggi Marilena , Cermisoni Greta Chiara , Ginesi Sara , Dovere Lisa , Albricci Laura , Guido Maurizio , Campitiello Maria Rosaria , Ferrero Susanna , Capalbo Antonio , Vaiarelli Alberto , Ubaldi Filippo Maria , Ferlin Alberto , Rienzi Laura , Gennarelli Gianluca 
Published: November 2024

Objective

To examine the association between semen parameters, assessed according to World Health Organization (WHO)‐2021 criteria, and paternal body mass index (BMI) and age, with embryological and clinical outcomes in ICSI cycles involving preimplantation genetic testing for aneuploidy (PGT‐A).

Design

Retrospective study at a private in vitro fertilization (IVF) clinic.

Subjects

3101 couples undergoing 4013 intracytoplasmic sperm injection (ICSI) + PGT‐A cycles with own‐oocytes (years 2013–2021).

Intervention

We performed trophectoderm biopsy, and comprehensive chromosome testing to report uniform aneuploidies and vitrified‐warmed euploid single‐blastocyst‐transfers. Regression analyses adjusted for relevant confounders were conducted to outline putative associations of semen analysis and characteristics and paternal BMI and age with all embryological/clinical outcomes.

Results

Maternal age was the only significant confounding variable affecting euploidy blastocyst rate (EBR) (primary embryological outcome). When categorized, motility < 5th‐percentile (‐2.5%, 95%CI ‐4.9 to ‐0.2%, p = 0.03), concentration plus morphology < 5th‐percentile (‐2.7%,95%CI ‐4.8 to ‐0.6%, p = 0.01), concentration plus morphology plus motility < 5th‐percentile (‐4.0%,95%CI ‐5.5 to ‐2.6%, p < 0.01), obstructive‐azoospermia [OA] (‐5.5%,95%CI ‐9 to ‐2%, p = 0.02) and non‐obstructive azoospermia (NOA) (‐5.8%,95%CI ‐10.9 to ‐0.6%, p = 0.03) showed significantly lower results compared to all parameters > 5th‐percentile. Furthermore, after adjusting for maternal age and the number of metaphase‐II‐oocytes inseminated, the only significant confounding variable affecting the chance of obtaining ≥ 1 live birth among completed cycles (primary clinical outcome) was basal and post sperm processing motility. When categorized, concentration plus morphology plus motility < 5th‐percentile (multivariable‐OR: 0.73, 95%CI 0.58–0.93, p = 0.01) and OA (multivariable‐OR: 0.47, 95%CI 0.24–0.92, p = 0.03) showed significantly lower chances compared to all parameters > 5th‐percentile. Advanced paternal age (defined as > 44 years) was associated only with lower day 5‐blastocyst and Gardner's AA‐grade (i.e., top quality) blastocyst rates.

Conclusions

This comprehensive analysis provides IVF professionals with useful figures to counsel infertile couples about their chances of success, taking into account the impact of semen characteristics and paternal BMI and age. These estimates are valuable for personalized decision‐making about the most effective reproductive strategies to adopt, especially not underestimating male factor, by improving sperm concentration and motility whenever possible before assisted reproductive technologies.