bluuebunny/crossref_metadata_2025 huggingface dataset:
Clear cell and papillary renal cell carcinomas in hereditary papillary renal cell carcinoma (HPRCC) syndrome: a case report
Authors: Ferlicot Sophie , Just Pierre-Alexandre , Compérat Eva , Rouleau Etienne , Tissier Frédérique , Vaessen Christophe , Richard Stéphane
Published: November 2021

Background

Hereditary papillary renal cell carcinoma (HPRCC) is a rare autosomal dominant disease characterized by the development of multiple and bilateral papillary type I renal cell carcinomas (RCC) and papillary adenomas caused by activating mutations in the MET proto-oncogene. Classically, distinctive histological features of RCC are described according to the familial renal cell carcinoma syndrome. To date, no clear cell RCC has been reported in HPRCC syndrome.

Case presentation

We describe the case of a 51-year-old man with a germline MET mutation detected on peripheral blood testing, and no germline VHL mutation, who developed numerous papillary tumors but also unexpectedly clear cell renal cell carcinomas. During the follow-up, an adrenal metastasis was observed 7 years after the initial diagnosis corresponding to a clear cell RCC metastasis. By immunohistochemistry, clear cell tumors showed focal cytokeratin 7, moderate racemase, and diffuse and membranous CAIX expression, while papillary tumors expressed strong diffuse cytokeratin 7 and racemase without CAIX positivity. Using FISH, VHL deletion was observed in one of the clear cell tumors, and the metastatic clear cell tumor presented a trisomy of chromosomes 7 and 17. These last genomic alterations are usually detected in papillary RCC, highlighting the potential link between both histological subtypes of tumors and the HPRCC syndrome.

Conclusions

The pathologist must be aware that the presence of a non-papillary RCC associated with numerous papillary tumors should not exclude the diagnostic suspicion of HPRCC and thus to perform a thorough genomic study.
Using Consolidated Framework for Implementation Research to investigate facilitators and barriers of implementing alcohol screening and brief intervention among primary care health professionals: a systematic review
Authors: Chan Paul Shing-fong , Fang Yuan , Wong Martin Chi-sang , Huang Junjie , Wang Zixin , Yeoh Eng Kiong
Published: November 2021

Background

Alcohol screening and brief intervention (SBI) is recommended to be implemented in primary care settings to intervene against hazardous/harmful drinking. However, studies showed that the uptake rate was low in many regions/countries. This systematic review presented current findings on the facilitators and barriers of SBI implemented by health professionals in primary care settings using the Consolidated Framework for Implementation Research (CFIR).

Methods

We included qualitative, quantitative, and mixed-method studies identified through four electronic databases (PubMed, MEDLINE, PsycInfo, and Web of Science) from inception to June 2020. Included articles had to address barriers and facilitators of SBI implementation and provide sufficient details that the CFIR domains could be identified and data were abstracted using a standardized extraction form.

Results

A total of 74 studies published from 1985 to 2019 were finally analysed and summarized. The most common facilitators were knowledge and positive beliefs about SBI (characteristics of the individuals) and available resources (inner setting). In contrast, the most common barriers were cost related to implementing SBI (intervention characteristics), negative beliefs about SBI (characteristics of the individuals), and lack of self-efficacy in implementing SBI (characteristics of the individuals). It could be observed that factors related to the inner setting and characteristics of individuals were extensively studied whilst the process received the least attention.

Conclusions

Most of the facilitators and barriers are modifiable. Additionally, most literature focused on various kinds of available assets to implement SBI. To promote the spread of SBI implementation, more high-quality studies on the implementation process are needed. This systematic review could serve as a reference framework for health authorities to devise strategies for improving the implementation of SBI in primary care settings.

Trial registration

This systematic review was registered in PROSPERO (CRD42021258833).
Impact of intraoperative medial collateral ligament injury on outcomes after total knee arthroplasty: a meta-analysis and systematic review
Authors: Li Jiahao , Yan Zijian , Lv Yan , Li Yijin , Ye Pengcheng , Deng Peng , Zhang Haitao , Chen Jinlun , Li Jie , Qi Xinyu , Zeng Jianchun , Zeng Yirong , Feng Wenjun
Published: November 2021

Background

As an uncommon but severe complication, medial collateral ligament (MCL) injury in total knee arthroplasty (TKA) may be significantly under-recognized. We aimed to determine whether MCL injury influences postoperative outcomes of patients undergoing TKA.

Methods

Two independent reviewers searched PubMed, Cochrane Library, and EMBASE from their inception to July 1, 2021. The main outcomes were postoperative function, and secondary outcomes included the incidences of revision and complications.

Results

A total of 403 articles yielded 15 studies eligible for inclusion with 10 studies used for meta-analysis. This study found that there was a statistically significant difference in postoperative functional scores, range of motion (ROM), complications, and revision rates, with adverse outcomes occurring more commonly in patients with MCL injury.

Conclusions

This meta-analysis highlights the complexity of MCL injury during TKA and shows the impact on postoperative function, joint mobility, complications, and revision. Surgeons need to prevent and put more emphasis on MCL injury during TKA.
A correlational study of Weifuchun and its clinical effect on intestinal flora in precancerous lesions of gastric cancer
Authors: Bian Yanqin , Chen Xi , Cao Hongyan , Xie Dong , Zhu Meiping , Yuan Nong , Lu Lu , Lu Bingjie , Wu Chao , Bahaji Azami Nisma Lena , Wang Zheng , Wang Huijun , Zhang Yeqing , Li Kun , Ye Guan , Sun Mingyu
Published: November 2021

Background

Weifuchun (WFC), a Chinese herbal prescription consisting of Red Ginseng, Isodon amethystoides and Fructus Aurantii, is commonly used in China to treat a variety of chronic stomach disorders. The aim of the paper was to determine the effect of WFC on intestinal microbiota changes in precancerous lesions of gastric cancer (PLGC) patients.

Methods

PLGC patients of H. pylori negative were randomly divided into two groups and received either WFC tablets for a dose of 1.44 g three times a day or vitacoenzyme (Vit) tablets for a dose of 0.8 g three times a day. All patients were treated for 6 months consecutively. Gastroscopy and histopathology were used to assess the histopathological changes in gastric tissues before and after treatment. 16S rRNA gene sequencing was carried out to assess the effects WFC on intestinal microbiota changes in PLGC patients. Receiver Operating Characteristics (ROC) analysis was used to assess the sensitivity and specificity of different intestinal microbiota in distinguishing between PLGC patients and healthy control group.

Results

Gastroscopy and histopathological results indicated that WFC could improve the pathological condition of PLGC patients, especially in the case of atrophy or intestinal metaplasia. The results of 16S rRNA gene sequencing indicated that WFC could regulate microbial diversity, microbial composition, and abundance of the intestinal microbiota of PLGC patients. Following WFC treatment, the relative abundance of Parabacteroides decreased in WFC group when compared with the Vit group. ROC analysis found that the Parabacteroides could effectively distinguish PLGC patients from healthy individuals with sensitivity of 0.79 and specificity of 0.8.

Conclusions

WFC could slow down the progression of PLGC by regulating intestinal microbiota abundance.

Trial registration NCT03814629. Name of registry: Randomized Clinical Trial: Weifuchun Treatment on Precancerous Lesions of Gastric Cancer. Registered 3 August 2018-Retrospectively registered, https://register.clinicaltrials.gov/ NCT03814629.
Retinal vessel tortuosity as a prognostic marker for disease severity in Fabry disease
Authors: Atiskova Yevgeniya , Wildner Jan , Spitzer Martin Stephan , Aries Charlotte , Muschol Nicole , Dulz Simon
Published: November 2021

Purpose

The aim of this case control study was to evaluate the prognostic value of automatically quantified retinal vessel tortuosity from fundus images and vessel density from OCT-A in Fabry disease and to evaluate the correlation of these with systemic disease parameters.

Methods

Automatically quantified perimacular retinal vessel tortuosity (MONA REVA software), acquired by fundus imaging, and perifoveal retinal vessel density, acquired by optic coherence tomography angiography (OCT-A) were compared between 26 FD patients and 26 controls. Gender and FD phenotype were analyzed to the obtained retinovascular data and correlated to the Mainz severity score index (MSSI) and plasma lyso-Gb3.

Results

Automatically quantified retinal vessel tortuosity indices of FD patients were significantly lower, reflecting an increased vessel tortuosity, compared to controls (p = 0.008). Males with a classical phenotype showed significantly lower retinal vessel tortuosity indices compared to males with an oligosymptomatic phenotype and females with a classical or oligosymptomatic phenotype (p < 0.001). The retinal vessel tortuosity index correlated significantly with systemic disease severity parameters [global MSSI (r = − 0.5; p < 0.01), cardiovascular MSSI (r = − 0.5; p < 0.01), lyso-Gb3 (r = − 0.6; p < 0.01)].

Conclusion

We advocate fundus imaging based automatically quantified retinal vessel tortuosity index over OCT-A imaging as it is a quick, non-invasive, easily assessable, objective and reproducible marker.
A Q study: exploring the purpose of transdisciplinary dairy advisory services in Denmark
Authors: Holm Wendy , Lastein Dorte Bay
Published: November 2021

Background

Structural changes in dairy farming increase farm complexity, thereby inducing a need to combine herd health management, technological solutions, legislation, and human relations among farmers, farm workers, and advisors. This complex situation may require ‘transdisciplinary advisory service’, i.e., a highly integrated network of both non-academic and different academic disciplines. While working in these networks, advisors need to offer specialized knowledge from their own field, interact in a dynamic relationship between different types of professions and facilitate complex processes. The objectives of this study were: (1) to identify and describe different advisor and farmer styles based on their reasons to engage in transdisciplinary advisory services at farm-level, (2) to identify any possible conflicting perspectives between advisors and farmers’ demand, and (3) to discuss these styles and conflicts in the context and future of advisory services for dairy herd health and production management.

Results

Using Q methodology, we explored the purpose of transdisciplinary advisory service on dairy farms. The results were derived from correlations between 40 statements for 25 advisors and 33 statements for nine farmers. We identified three similar styles among advisors and farmers, characterized as: (1)the teamwork and knowledge-focused style, (2)the production and economy-focused style, and (3)the economy and strategy-focused style. These styles included reflections on financial aspects, production, knowledge-exchange and the teamwork process itself. In addition, different emphasis on animal welfare, farm strategy and follow-up procedures between the styles became evident.

Conclusions

This Q-study suggests three comparable styles between advisors and farmers. The main differences between the styles related to the teamwork process and purpose, follow-up process, financial aspects, farm strategy, and operational production objectives. Therefore, styles and expectations should be explored and discussed to create a mutual understanding within a farmer-advisor(s)-team, and to clarify the farmer’s needs and demands, and how the advisors can best meet these expectations. This study illustrates the importance of exploring different advisor and farmer styles to get a mutual understanding of the purpose of the transdisciplinary collaboration.
The effectiveness of Pictorial Representation of Illness and Self Measure (PRISM) for the assessment of the suffering and quality of interpersonal relationships of patients with chronic pain
Authors: Tomioka Mitsunao , Hosoi Masako , Okuzawa Tomona , Anno Kozo , Iwaki Rie , Kawata Hiroshi , Kubo Chiharu , Sudo Nobuyuki
Published: November 2021

Background

Pictorial Representation of Illness and Self Measure (PRISM) is a tool that can be used to visualize and evaluate the burden of suffering caused by an illness. The aim of this study was to identify which aspects of the burden of chronic pain patients are associated with Self/illness separation (SIS), an indicator of the magnitude of suffering. We also examined the effectiveness of PRISM for evaluating changes in the relationships between patients and their medical care and significant others due to our inpatient treatment.

Methods

Seventy-two patients with chronic pain who were outpatients or admitted to the Department of Psychosomatic Medicine completed PRISM, depression and anxiety scales, and three types of pain-related self-assessment questionnaires (Brief Pain Inventory, Short-form McGill Pain Questionnaire, and Pain Catastrophizing Scale). Outpatients were queried at the time of outpatient visits and inpatients at the time of admission. In addition to PRISM disks related to illness, we asked each patient to place disks related to things important to them and their medical care. Of the inpatients, 31 did PRISM at the time of discharge. Among the reported important factors, which significant other was placed at the time of admission and discharge was evaluated. The distances of self/medical care separation (SMcS) and self/significant others separation (SSoS) were measured.

Results

Of the 21 scales measured, 10 showed a significant correlation with SIS. Factor analysis of these 10 scales extracted three factors, Life interferences, Negative affects, and Pain intensity. The SMcS and SSoS distances were shorter at discharge than at admission.

Conclusions

PRISM for patients with chronic pain is an integrated evaluation method that reflects three aspects of pain. By adding medical care and significant others to the usual method of placing only illness on the sheet it became possible to assess changes in the quality of interpersonal relationships.
Gender-based violence and infectious disease in humanitarian settings: lessons learned from Ebola, Zika, and COVID-19 to inform syndemic policy making
Authors: Meinhart Melissa , Vahedi Luissa , Carter Simone E. , Poulton Catherine , Mwanze Palaku Philomene , Stark Lindsay
Published: November 2021

Background

The impacts of infectious disease outbreaks, epidemics, and pandemics are not gender neutral. Instead, infectious diseases and gender-based violence (GBV) mutually reinforce each other. Women and girls in humanitarian settings are disproportionately impacted as crises exacerbate gender inequality, violence, and community transmission. A syndemic model of infectious disease and GBV draws attention to their critical linkage, enabling more effective approaches to address both infectious disease transmission and GBV prevalence.

Main body

Implementation of infectious disease control measures have been consistently absent of critical gender considerations in humanitarian settings. We drew learnings from Ebola, Zika, and COVID-19 to highlight how women and girls living in humanitarian settings have faced bi-directional syndemic vulnerabilities between GBV and infectious disease. Our findings indicate that Ebola, Zika, and COVID-19 exacerbated GBV risk and experience of GBV increased community transmission of these infectious diseases. Moreover, we identified a failure of existing policies to address this mutually deleterious linkage. Thus, we advocate for policymakers to ask three foundational questions: (i) What are the gendered bi-directional risk pathways between infectious disease and GBV?; (ii) How can we act on the gendered risk pathways?; and, (iii) Who should be involved in designing, implementing, and evaluating gender-sensitive policies?

Conclusion

Our syndemic policy framework challenges existing thinking on a neglected issue that disproportionally impacts women and girls. By offering foundational guidance to address and thwart the syndemic of infectious disease and GBV in humanitarian settings, we endeavor to proactively and holistically address the reinforcing linkage between GBV and current or emergent infectious diseases.
Zebrafish xenograft model for studying mechanism and treatment of non-small cell lung cancer brain metastasis
Authors: Fan Ruo-Yue , Wu Jia-Qi , Liu Yu-Yang , Liu Xiang-Yu , Qian Si-Tong , Li Chong-Yong , Wei Ping , Song Zhe , He Ming-Fang
Published: November 2021

Background

Brain metastasis (BM) is thought to be related to the mortality and poor prognosis of non-small cell lung cancer (NSCLC). Despite promising development of NSCLC treatment, the treatment of NSCLC BM is still not optimistic due to the existence of the blood-brain barrier (BBB) that prevent drug penetration, as well as the short median survival time of the patients left for treatment. In this context, further development of quick and effective pre-clinical models is needed in NSCLC BM treatment. Here, we report a model system using zebrafish to promote the development of drugs for patients with NSCLC BM.

Methods

Three different NSCLC cell lines (H1975, A549 and H1299) were used to establish zebrafish BM models. The embryo age and cell number for injection were first optimized. Metastatic cells were observed in the brain blood vessels of zebrafish and were verified by hematoxylin-eosin (HE) staining. Then, the metastasis potentials of H1975 and A549 with manipulated microRNA-330-3p (miR-330-3p) expression were also investigated. Finally, sensitivities of H1975 and A549 to osimertinib and gefitinib were tested.

Results

This zebrafish BM model could distinguish NSCLC cell lines with different BM potential. Over-expressed miR-330-p significantly improved the BM potential of the A549 cells while knockdown miR-330-p reduced the BM ability of the H1975 cells. Both osimertinib and gefitinib showed inhibition effect in zebrafish BM model with the inhibition rate higher than 50 %. H1975 cell showed much higher sensitivity to osimertinib rather than gefitinib both in vivo and in vitro.

Conclusions

We established zebrafish brain metastasis model for studying mechanism and treatment of NSCLC BM. This study provided a useful model for NSCLC brain metastasis that could be used to study the mechanism that drive NSCLC cells to the brain as well as identify potential therapeutic options.
Helicopter emergency medical services use of thoracic point of care ultrasound for pneumothorax: a systematic review and meta-analysis
Authors: Griffiths Edward
Published: November 2021

Background

Auscultating for breath sounds to assess for pneumothorax in the helicopter emergency medical services (HEMS) settings can be extremely challenging. Thoracic point of care ultrasound (POCUS) offers a seemingly more useful visual (rather than audible) alternative. This review critically and quantitatively evaluates the use of thoracic POCUS for pneumothorax in the HEMS setting.

Methods

A systematic literature review with meta-analysis was conducted. Only papers reporting on patients undergoing POCUS for pneumothorax in the helicopter or pre-hospital setting were included. Primary outcome was accuracy, focusing on sensitivity and specificity. Secondary outcome was practicality. PubMed, Embase and the Cochrane Library were searched. The Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) was used to assess validity of studies.

Results

Twelve studies reporting on n = 1,936 images from medical and trauma patients were included in qualitative synthesis. Studies were nearly all observational designs. Most images were acquired by nurses or paramedics who were previously novices to ultrasound. The reference standard was predominantly CT. Specificity results were unanimously precise and very high, whereas sensitivity results were imprecise and extremely variable. Meta-analysis of eight studies involving n = 1,713 images yielded pooled sensitivity 61% (95% CI: 27–87%; I2 = 94%) and pooled specificity 99% (95% CI: 98–100%; I2 = 89%). Six studies involving n = 315 images reported practicality. The highest or second highest categorisation of image quality was reported in around half of those images.

Conclusion

Thoracic POCUS is highly specific but has extremely variable sensitivity for pneumothorax when performed in the HEMS setting. This is from purely a diagnostic (not clinical) perspective. Sensitivity increases when only clinically significant pneumothoraces are considered. Case reports reveal thoracic POCUS can appropriately alter treatment and triage decisions, but only for a small number of patients. It appears predominantly useful in mitigating against unnecessary interventions. More research reporting patient focused outcomes is required. In the meantime, thoracic POCUS appears to offer a more appropriate visual alternative to auscultation for breath sounds when assessing for pneumothorax in the HEMS setting.
Safety and efficacy of intermittent presumptive treatment with sulfadoxine-pyrimethamine using rapid diagnostic test screening and treatment with dihydroartemisinin-piperaquine at the first antenatal care visit (IPTp-SP+): study protocol for a randomized controlled trial
Authors: Kabuya Jean-Bertin Bukasa , Ippolito Matthew M. , Sikalima Jay , Tende Clifford , Champo Davies , Mwakazanga David , Young Anna Marie P. , Mulenga Modest , Chongwe Gershom , Manyando Christine
Published: November 2021

Background

Intermittent preventive treatment in pregnancy (IPTp) with sulfadoxine-pyrimethamine (SP) is recommended by the World Health Organization for the prevention of malaria in pregnancy (MIP)-associated adverse outcomes in high burden areas. However, the efficacy of IPTp-SP has decreased in step with increasing parasite drug resistance. Suitable alternative strategies are needed.

Methods

This is a protocol for a phase IIIb open-label, two-armed randomized controlled superiority trial to assess the safety and efficacy of a hybrid approach to IPTp combining screening and treatment with dihydroartemisinin-piperaquine (DP) to the current IPTp-SP regimen at the first antenatal care clinic visit. Pregnant women without HIV infection and without signs or symptoms of malaria will be randomized to either standard IPTp-SP or hybrid IPTp-SP plus screening and treatment (IPTp-SP+). In the IPTp-SP+ arm, participants who screen positive by rapid diagnostic test for P. falciparum will be treated with DP at the first antenatal visit while those who screen negative will receive SP per current guidelines. All participants will be administered SP on days 35 and 63 and will be actively followed biweekly up to day 63 and then monthly until delivery. Infants will be followed until 1 year after delivery. The primary endpoint is incident PCR-confirmed MIP at day 42. Secondary endpoints include incident MIP at other time points, placental malaria, congenital malaria, hemoglobin trends, birth outcomes, and incidence of adverse events in infants up to the first birthday.

Discussion

A hybrid approach to IPTp that combines screening and treatment with an artemisinin-based combination therapy at the first visit with standard IPTp-SP is hypothesized to confer added benefit over IPTp-SP alone in a high malaria transmission area with prevalent SP resistant parasites.

Trial registration

Pan African Clinical Trials Registry 201905721140808. Registered retrospectively on 11 May 2019
A randomized controlled trial of self-management for people with epilepsy and a history of negative health events (SMART) targeting rural and underserved people with epilepsy: a methodologic report
Authors: Ghearing Gena R. , Briggs Farren , Cassidy Kristin , Privitera Michael , Blixen Carol , Sajatovic Martha
Published: November 2021

Background

Many people living with epilepsy (PLWE) reside in rural communities, and epilepsy self-management may help address some of the gaps in epilepsy care for these communities. A prior randomized control trial of a remotely delivered, Web-based group format 12-week self-management program (SMART) conducted in Northeast Ohio, a primarily urban and suburban community, demonstrated improved outcomes in negative health events such as depression symptoms and quality of life. However, there is a paucity of research addressing the needs of PLWE in rural settings.

Methods

The present study leverages collaboration between investigators from 2 mid-western US states (Ohio and Iowa) to replicate testing of the SMART intervention and prioritize delivery to PLWE in rural and semi-rural communities. In phase 1, investigators will refine the SMART program using input from community stakeholders. A Community Advisory Board will then be convened to help identify barriers to trial implementation and strategies to overcome barriers. In phase 2, the investigators will conduct a 6-month prospective randomized control trial of the SMART program compared to 6-month waitlist controls, with the primary outcome being changes in negative health events defined as seizure, self-harm attempt, emergency department visit, or hospitalization. Additional outcomes of interest include quality of life and physical and mental health functioning. The study will also assess process measures of program adopters and system end-users to inform future outreach, education, and self-management strategies for PLWE.

Discussion

The method of this study employs lived experience of PLWE and those who provide care for PLWE in rural and underserved populations to refine a remotely delivered Web-based self-management program, to improve recruitment and retention, and to deliver the intervention. Pragmatic outcomes important to PLWE, payers, and policymakers will be assessed. This study will provide valuable insights on implementing future successful self-management programs.

Trial registration

ClinicalTrials.govNCT04705441. Registered on January 12, 2021
When metabolic prowess is too much of a good thing: how carbon catabolite repression and metabolic versatility impede production of esterified α,ω-diols in Pseudomonas putida KT2440
Authors: Lu Chunzhe , Batianis Christos , Akwafo Edward Ofori , Wijffels Rene H. , Martins dos Santos Vitor A. P. , Weusthuis Ruud A.
Published: November 2021

Background

Medium-chain-length α,ω-diols (mcl-diols) are important building blocks in polymer production. Recently, microbial mcl-diol production from alkanes was achieved in E. coli (albeit at low rates) using the alkane monooxygenase system AlkBGTL and esterification module Atf1. Owing to its remarkable versatility and conversion capabilities and hence potential for enabling an economically viable process, we assessed whether the industrially robust P. putida can be a suitable production organism of mcl-diols.

Results

AlkBGTL and Atf1 were successfully expressed as was shown by oxidation of alkanes to alkanols, and esterification to alkyl acetates. However, the conversion rate was lower than that by E. coli, and not fully to diols. The conversion was improved by using citrate instead of glucose as energy source, indicating that carbon catabolite repression plays a role. By overexpressing the activator of AlkBGTL-Atf1, AlkS and deleting Crc or CyoB, key genes in carbon catabolite repression of P. putida increased diacetoxyhexane production by 76% and 65%, respectively. Removing Crc/Hfq attachment sites of mRNAs resulted in the highest diacetoxyhexane production. When the intermediate hexyl acetate was used as substrate, hexanol was detected. This indicated that P. putida expressed esterases, hampering accumulation of the corresponding esters and diesters. Sixteen putative esterase genes present in P. putida were screened and tested. Among them, Est12/K was proven to be the dominant one. Deletion of Est12/K halted hydrolysis of hexyl acetate and diacetoxyhexane. As a result of relieving catabolite repression and preventing the hydrolysis of ester, the optimal strain produced 3.7 mM hexyl acetate from hexane and 6.9 mM 6-hydroxy hexyl acetate and diacetoxyhexane from hexyl acetate, increased by 12.7- and 4.2-fold, respectively, as compared to the starting strain.

Conclusions

This study shows that the metabolic versatility of P. putida, and the associated carbon catabolite repression, can hinder production of diols and related esters. Growth on mcl-alcohol and diol esters could be prevented by deleting the dominant esterase. Carbon catabolite repression could be relieved by removing the Crc/Hfq attachment sites. This strategy can be used for efficient expression of other genes regulated by Crc/Hfq in Pseudomonas and related species to steer bioconversion processes.
Overexpression of the rice BAHD acyltransferase AT10 increases xylan-bound p-coumarate and reduces lignin in Sorghum bicolor
Authors: Tian Yang , Lin Chien-Yuan , Park Joon-Hyun , Wu Chuan-Yin , Kakumanu Ramu , Pidatala Venkataramana R. , Vuu Khanh M. , Rodriguez Alberto , Shih Patrick M. , Baidoo Edward E. K. , Temple Stephen , Simmons Blake A. , Gladden John M. , Scheller Henrik V. , Eudes Aymerick
Published: November 2021

Background

The development of bioenergy crops with reduced recalcitrance to enzymatic degradation represents an important challenge to enable the sustainable production of advanced biofuels and bioproducts. Biomass recalcitrance is partly attributed to the complex structure of plant cell walls inside which cellulose microfibrils are protected by a network of hemicellulosic xylan chains that crosslink with each other or with lignin via ferulate (FA) bridges. Overexpression of the rice acyltransferase OsAT10 is an effective bioengineering strategy to lower the amount of FA involved in the formation of cell wall crosslinks and thereby reduce cell wall recalcitrance. The annual crop sorghum represents an attractive feedstock for bioenergy purposes considering its high biomass yields and low input requirements. Although we previously validated the OsAT10 engineering approach in the perennial bioenergy crop switchgrass, the effect of OsAT10 expression on biomass composition and digestibility in sorghum remains to be explored.

Results

We obtained eight independent sorghum (Sorghum bicolor (L.) Moench) transgenic lines with a single copy of a construct designed for OsAT10 expression. Consistent with the proposed role of OsAT10 in acylating arabinosyl residues on xylan with p-coumarate (pCA), a higher amount of p-coumaroyl-arabinose was released from the cell walls of these lines upon hydrolysis with trifluoroacetic acid. However, no major changes were observed regarding the total amount of pCA or FA esters released from cell walls upon mild alkaline hydrolysis. Certain diferulate (diFA) isomers identified in alkaline hydrolysates were increased in some transgenic lines. The amount of the main cell wall monosaccharides glucose, xylose, and arabinose was unaffected. The transgenic lines showed reduced lignin content and their biomass released higher yields of sugars after ionic liquid pretreatment followed by enzymatic saccharification.

Conclusions

Expression of OsAT10 in sorghum leads to an increase of xylan-bound pCA without reducing the overall content of cell wall FA esters. Nevertheless, the amount of total cell wall pCA remains unchanged indicating that most pCA is ester-linked to lignin. Unlike other engineered plants overexpressing OsAT10 or a phylogenetically related acyltransferase with similar putative function, the improvements of biomass saccharification efficiency in sorghum OsAT10 lines are likely the result of lignin reductions rather than reductions of cell wall-bound FA. These results also suggest a relationship between xylan-bound pCA and lignification in cell walls.
The association between FTO rs9939609 polymorphism and serum lipid profile in adult women
Authors: Jalili Vahideh , Mokhtari Zohreh , Rastgoo Samira , Hajipour Azadeh , Bourbour Fatemeh , Gholamalizadeh Maryam , Mosavi Jarrahi Alireza , JavadiKooshesh Sepehr , Moslem Alireza , Abdollahi Morteza , Doaei Saeid
Published: November 2021

Background

FTO gene is considered to play an important role in many metabolic diseases. Evidence from studies indicated the possible association between the FTO rs9939609 polymorphisms with serum lipid profile. Therefore, this study aimed to investigate the association of FTO rs9939609 polymorphism with lipid profile in Iranian women.

Methods

This cross-sectional study was carried out on 380 adult women. Information about age, height, weight, BMI, physical activity, and dietary intake were collected. The serum levels of Low Density Lipoprotein (LDL), High Density Lipoprotein (HDL), Triglyceride (TG), and total cholesterol were measured. The FTO gene was genotyped for rs9939609 polymorphism. The participants were divided into two groups of TT and AT/AA considering dominant model of FTO rs9939609 polymorphism.

Results

General characteristics of the participants with different FTO genotypes were not significantly different. The lower levels of HDL were observed in AT/AA genotypes compared to the TT wild type genotype of FTO rs9939609 polymorphism (P = 0.004). Adjustments of age, BMI, and physical activity did not change the results.

Conclusions

However, the significant association between FTO genotype and the HDL level was disappeared after further adjustments for dietary intake. Further studies are warranted to identify the underlying mechanisms of the possible association between FTO gene and serum lipid profile.
Machine learning-based CT radiomics approach for predicting WHO/ISUP nuclear grade of clear cell renal cell carcinoma: an exploratory and comparative study
Authors: Xv Yingjie , Lv Fajin , Guo Haoming , Zhou Xiang , Tan Hao , Xiao Mingzhao , Zheng Yineng
Published: November 2021

Purpose

To investigate the predictive performance of machine learning-based CT radiomics for differentiating between low- and high-nuclear grade of clear cell renal cell carcinomas (CCRCCs).

Methods

This retrospective study enrolled 406 patients with pathologically confirmed low- and high-nuclear grade of CCRCCs according to the WHO/ISUP grading system, which were divided into the training and testing cohorts. Radiomics features were extracted from nephrographic-phase CT images using PyRadiomics. A support vector machine (SVM) combined with three feature selection algorithms such as least absolute shrinkage and selection operator (LASSO), recursive feature elimination (RFE), and ReliefF was performed to determine the most suitable classification model, respectively. Clinicoradiological, radiomics, and combined models were constructed using the radiological and clinical characteristics with significant differences between the groups, selected radiomics features, and a combination of both, respectively. Model performance was evaluated by receiver operating characteristic (ROC) curve, calibration curve, and decision curve analyses.

Results

SVM-ReliefF algorithm outperformed SVM-LASSO and SVM-RFE in distinguishing low- from high-grade CCRCCs. The combined model showed better prediction performance than the clinicoradiological and radiomics models (p < 0.05, DeLong test), which achieved the highest efficacy, with an area under the ROC curve (AUC) value of 0.887 (95% confidence interval [CI] 0.798–0.952), 0.859 (95% CI 0.748–0.935), and 0.828 (95% CI 0.731–0.929) in the training, validation, and testing cohorts, respectively. The calibration and decision curves also indicated the favorable performance of the combined model.

Conclusion

A combined model incorporating the radiomics features and clinicoradiological characteristics can better predict the WHO/ISUP nuclear grade of CCRCC preoperatively, thus providing effective and noninvasive assessment.
Cell therapy as a treatment of secondary lymphedema: a systematic review and meta-analysis
Authors: Lafuente Hector , Jaunarena Ibon , Ansuategui Eukene , Lekuona Arantza , Izeta Ander
Published: November 2021

Background

Lymphedema, the accumulation of interstitial fluid caused by poor lymphatic drainage, is a progressive and permanent disease with no curative treatment. Several studies have evaluated cell-based therapies in secondary lymphedema, but no meta-analysis has been performed to assess their efficacy.

Methods

We conducted a systematic review and meta-analysis of all available preclinical and clinical studies, with assessment of their quality and risk of bias.

Results

A total of 20 articles using diverse cell types were selected for analysis, including six clinical trials and 14 pre-clinical studies in three species. The meta-analysis showed a positive effect of cell-based therapies on relevant disease outcomes (quantification of edema, density of lymphatic capillaries, evaluation of the lymphatic flow, and tissue fibrosis). No significant publication bias was observed.

Conclusion

Cell-based therapies have the potential to improve secondary lymphedema. The underlying mechanisms remain unclear. Due to relevant heterogeneity between studies, further randomized controlled and blinded studies are required to substantiate the use of these novel therapies in clinical practice.
Sex and gender considerations in reporting guidelines for health research: a systematic review
Authors: Gogovor Amédé , Zomahoun Hervé Tchala Vignon , Ekanmian Giraud , Adisso Évèhouénou Lionel , Deom Tardif Alèxe , Khadhraoui Lobna , Rheault Nathalie , Moher David , Légaré France
Published: November 2021

Background

Despite growing recognition of the importance of sex and gender considerations in health research, they are rarely integrated into research design and reporting. We sought to assess the integration of sex, as a biological attribute, and gender, as a socially constructed identity, in published reporting guidelines.

Methods

We conducted a systematic review of published reporting guidelines listed on the EQUATOR website (www.equator-nework.org) from inception until December 2018. We selected all reporting guidelines (original and extensions) listed in the EQUATOR library. We used EndNote Citation Software to build a database of the statements of each guideline identified as a "full bibliographic reference" and retrieved the full texts. Reviewers independently extracted the data on use of sex and gender terms from the checklist/abstract/main text of guidelines. Data were analyzed using descriptive statistics and narrative synthesis.

Results

A total of 407 reporting guidelines were included; they were published between 1995 and 2018. Of the 407 guidelines, 235 (57.7%) mentioned at least one of the sex- and gender-related words. In the checklist of the reporting guidelines (n = 363), “sex” and “gender” were mentioned in 50 (13.8%) and 40 (11%), respectively. Only one reporting guideline met our criteria (nonbinary, appropriate categorization, and non-interchangeability) for correct use of sex and gender concepts. Trends in the use of "sex" and "gender" in the checklists showed that the use of “sex” only started in 2003, while “gender” has been in use since 1996.

Conclusions

We assessed the integration of sex and gender in reporting guidelines based on the use of sex- and gender-related words. Our findings showed a low use and integration of sex and gender concepts and their incorrect use. Authors of reporting guidelines should reduce this gap for a better use of research knowledge.

Trial registration PROSPERO no. CRD42019136491.
Deleting qseC downregulates virulence and promotes cross-protection in Pasteurella multocida
Authors: Yang Yang , Hu Pei , Gao Lixu , Yuan Xiang , Hardwidge Philip R. , Li Tian , Li Pan , He Fang , Peng Yuanyi , Li Nengzhang
Published: November 2021

QseC, a histidine sensor kinase of the QseBC two-component system, acts as a global regulator of bacterial stress resistance, biofilm formation, and virulence. The function of QseC in some bacteria is well understood, but not inPasteurella multocida. We found that deletingqseCinP. multocidaserotype A:L3 significantly down-regulated bacterial virulence. The mutant had significantly reduced capsule production but increased resistance to oxidative stress and osmotic pressure. DeletingqseCled to a significant increase inqseBexpression. Transcriptome sequencing analysis showed that 1245 genes were regulated byqseC, primarily those genes involved in capsule and LPS biosynthesis and export, biofilm formation, and iron uptake/utilization, as well as several immuno-protection related genes includingompA,ptfA,plpB,vacJ, andsodA. In addition to presenting strong immune protection againstP. multocidaserotypes A:L1 and A:L3 infection, live ΔqseCalso exhibited protection againstP. multocidaserotype B:L2 and serotype F:L3 infection in a mouse model. The results indicate that QseC regulates capsular production and virulence inP. multocida. Furthermore, theqseCmutant can be used as an attenuated vaccine againstP. multocidastrains of multiple serotypes.
RETRACTED ARTICLE: Real-time detection of aerobics posture based on strain sensor
Authors: Zhu Shanshan
Published: November 2021

The Internet of Things is a network that realizes the intelligent connection between things. It is another major industry after the computer industry and the Internet industry. It is also a collection of computer technology, communication technology, sensor technology, storage technology, and many other leading technologies in an integrated industry, the application industry is very wide. This article mainly introduces the real-time detection of aerobics posture based on strain sensors. This paper proposes a real-time detection method of motion posture based on strain sensors, using flexible rods to simulate the joints of aerobics athletes. Through the survey of the motion posture by the strain sensor, the relevant data and parameters are collected, and then the data are processed, and the data are finally transmitted to the terminal. Since the stress of a specific point on the surface of the flexible rod is proportional to the curve of that point, strain gauges can be used to detect curve changes at multiple points in real time and draw the change curve on the computer. The experimental structure of this paper shows that the use of BP neural network to process the measurement data improves the accuracy of real-time detection. The error distance between the sampling point and the actual value is less than 0.3 cm. In addition, through the real-time detection of the motion posture, it can be concluded that good Body posture is the basic requirement of aerobics, and it is of great significance to improve the effect of improving the quality of movement and artistic expression.
A multichannel learning-based approach for sound source separation in reverberant environments
Authors: Chen You-Siang , Lin Zi-Jie , Bai Mingsian R.
Published: November 2021

In this paper, a multichannel learning-based network is proposed for sound source separation in reverberant field. The network can be divided into two parts according to the training strategies. In the first stage, time-dilated convolutional blocks are trained to estimate the array weights for beamforming the multichannel microphone signals. Next, the output of the network is processed by a weight-and-sum operation that is reformulated to handle real-valued data in the frequency domain. In the second stage, a U-net model is concatenated to the beamforming network to serve as a non-linear mapping filter for joint separation and dereverberation. The scale invariant mean square error (SI-MSE) that is a frequency-domain modification from the scale invariant signal-to-noise ratio (SI-SNR) is used as the objective function for training. Furthermore, the combined network is also trained with the speech segments filtered by a great variety of room impulse responses. Simulations are conducted for comprehensive multisource scenarios of various subtending angles of sources and reverberation times. The proposed network is compared with several baseline approaches in terms of objective evaluation matrices. The results have demonstrated the excellent performance of the proposed network in dereverberation and separation, as compared to baseline methods.
Positive solutions for eigenvalue problems of fractional q-difference equation with ϕ-Laplacian
Authors: Wang Jufang , Yu Changlong , Zhang Boya , Wang Si
Published: November 2021

The aim of this paper is to investigate the boundary value problem of a fractional q-difference equation with ϕ-Laplacian, where ϕ-Laplacian is a generalized p-Laplacian operator. We obtain the existence and nonexistence of positive solutions in terms of different eigenvalue intervals for this problem by means of the Green function and Guo–Krasnoselskii fixed point theorem on cones. Finally, we give some examples to illustrate the use of our results.
A price-aware congestion control protocol for cloud services
Authors: Sun Xiaocui , Wang Zhijun , Wu Yunxiang , Che Hao , Jiang Hong
Published: November 2021

In current infrastructure-as-a service (IaaS) cloud services, customers are charged for the usage of computing/storage resources only, but not the network resource. The difficulty lies in the fact that it is nontrivial to allocate network resource to individual customers effectively, especially for short-lived flows, in terms of both performance and cost, due to highly dynamic environments by flows generated by all customers. To tackle this challenge, in this paper, we propose an end-to-end Price-Aware Congestion Control Protocol (PACCP) for cloud services. PACCP is a network utility maximization (NUM) based optimal congestion control protocol. It supports three different classes of services (CoSes), i.e., best effort service (BE), differentiated service (DS), and minimum rate guaranteed (MRG) service. In PACCP, the desired CoS or rate allocation for a given flow is enabled by properly setting a pair of control parameters, i.e., a minimum guaranteed rate and a utility weight, which in turn, determines the price paid by the user of the flow. Two pricing models, i.e., a coarse-grained VM-Based Pricing model (VBP) and a fine-grained Flow-Based Pricing model (FBP), are proposed. The optimality of PACCP is verified by both large scale simulation and small testbed implementation. The price-performance consistency of PACCP are evaluated using real datacenter workloads. The results demonstrate that PACCP provides minimum rate guarantee, high bandwidth utilization and fair rate allocation, commensurate with the pricing models.