bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Bacterial Peptide p28 on Viability and Apoptosis Statusof P53-null HeLa Cells
Authors: Abuei Haniyeh , Behzad-Behbahani Abbas , Faghihi Fatemeh , Farhadi Ali , Rafiei Dehbidi Gholam Reza , Pirouzfar Mohammad , Zare Farahnaz
Published: November 2019

Purpose: Despite all the efforts for discovery of efficient anti-cancer therapeutics, cancer is stilla major health concern worldwide. p28 is a bacterial small peptide which has been widelyinvestigated due to its preferential cell internalization and anti-cancer activities. Intracellularly,p28 offers its anti-cancer traits by impeding the degradation of tumor-suppressor protein "p53".In this study, we investigated the potency of p28 in inducing apoptosis or decreasing cellviability in p53-null "HeLa" cell line.Methods: The coding sequence for p28 peptide was obtained from Pseudomonas aeruginosaby PCR amplification of the p28 gene. The coding gene was cloned in pET-28a vector andtransformed into E. coli bacterial host. Subsequently, the expressed peptide was purified usingNi-NTA chromatography system and introduced into the target cells. The anti-proliferative andapoptotic activity of p28 on HeLa and HEK-293 cells were investigated using MTT and PEAnnexinV Flowcytometry assays.Results: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Westernblotting confirmed the expression of p28 peptide in the bacterial host. Bradford assay revealeda concentration of 0.05 mg/mL for the purified p28. MTT assay of cells treated with p28 atconcentrations of 0, 0.5, 1, 2 and 2.5 μM indicated 24h viability values of 97%, 89%, 88%,87% and 84% for HeLa cells, respectively. Data obtained from flowcytometry analyses revealed24h apoptosis rate of 7.17%, 8.05%, 8.63% and 8.84% for HeLa cells treated with 0, 0.5, 1,and 2 μM p28, respectively.Conclusion : MTT and flowcytometry apoptosis assays suggest no statistically significant effectof p28 on the viability and apoptosis status of p53-null HeLa cells when results compared todata obtained from HEK-293 cells (P > 0.05). These results imply that anti-cancer efficacy of p28is directly dependent on the presence of p53, suggesting p28 as an inappropriate therapeuticagent for treatment of cancers with negative p53 status.
Molecular Docking and Preclinical Study of Five-MemberedS,S-Palladaheterocycle as Hepatoprotective Agent
Authors: Akhmadiev Nail Salavatovich , Galimova Albina Midkhatovna , Akhmetova Vnira Rakhimovna , Khairullina Veronika Radievna , Galimova Rozaliia Akramovna , Agletdinov Eduard Feliksovich , Ibragimov Askhat Gabdrahmanovich , Kataev Valery Alekseevich
Published: November 2019

Purpose: In order to investigate mechanisms underlying the hepatoprotective action of S,Spalladaheterocycle,inhibition of cytochromes P450 has been modeled by molecular dockingof four palladaheterocycle stereoisomers to the active sites of an enzymatic oxidase system. Toobtain a deeper insight into biochemical aspects providing a basis for the therapeutic effects offive-membered palladacycles (as mixture of stereoisomers), a number of preclinical trials hasbeen conductedMethods: 2D and 3D structures of palladaheterocycle stereoisomers were obtained viaconverting into SDF files by means of software MarvinSketch. Binding of palladaheterocycle atthe active sites of cytochromes P450 2E1 and P450 2C9 has been studied by molecular dockingusing LeadIT 2.3.2. Hepatoprotective activity of palladaheterocycle at 2.5, 25 and 250 mg/kgdoses has been studied based on a model of acute intoxication by CCl4 using in vivo methods.Results: By molecular docking it was identify amino acid fragments responsible for bindingwith palladacyclic isomers. The tested compound is comparable, in terms of its activity tothe hepatoprotective drug SAM according to the in vivo and in vitro experiments such asanimal survival data, the efficiency of correction of the cytolytic syndrome, the liver excretoryfunction, carbohydrate, protein and lipid metabolism, and the correction efficiency of the liverantitoxic function (the latter has been determined based on the results of a hexobarbital controlexperiment).Conclusion: Taking into account results obtained in vivo, in vitro and in silico, it can be concludedthat the five-membered S,S-palladaheterocycle effectively protect the liver against acute damagecaused by CCl4, via activation of catalase and glucuronyltransferase, as well as via inhibition ofthe oxidative stress enzymes.
Social Determinants of Polypharmacy in First Generation Mexican Immigrants in the United States
Authors: Assari Shervin , Saqib Mohammed , Wisseh Cheryl , Bazargan Mohsen
Published: November 2019

Introduction: Socioeconomic status (SES) indicators are among the main social determinants of health and illness. Less, however, is known about the role of SES in the epidemiology of polypharmacy in immigrant Latino Americans living in the United States. This research studied the association between three SES indicators, education, income, and employment, and polypharmacy in older first generation Latino American immigrant adults. Methods: Data was obtained from the Sacramento Area Latino Study on Aging (SALSA, 1996-2008). A total of 632 older first generation Mexican-American immigrants to the U.S. entered this analysis. The independent variables were education, income, and employment. Polypharmacy was the outcome. Age, gender, physical health, smoking, and drinking were the covariates. Binary logistic regression was used to analyze the data. Results: Employment was associated with lower odds of polypharmacy. The association between education and polypharmacy was above and beyond demographic factors, physical health, health behaviors, and health insurance. Neither education nor income were associated with polypharmacy. Other determinants of polypharmacy were poor self-rated health (SRH) and a higher number of chronic medical conditions (CMCs). Conclusion: Employment appears to be the major SES determinant of polypharmacy in older foreign-born Mexican Americans. Unemployed older Mexican American immigrants with multiple chronic diseases and those who have poor SRH have the highest need for an evaluation of polypharmacy. Given the age group of this population, most of them have health insurance, which provides an opportunity for reducing their polypharmacy.
Protective Effects of Educational Attainment Against Cigarette Smoking; Diminished Returns of American Indians and Alaska Natives in the National Health Interview Survey
Authors: Assari Shervin , Bazargan Mohsen
Published: November 2019

Introduction: Although educational attainment is protective against health risk behaviors such as smoking, the Minorities’ Diminished Returns theory posits that these protective effects are smaller for ethnic minorities than majority groups. This study compared the effects of educational attainment on the smoking status of American Indian/Alaska Native (AIAN) and White adults. Methods: Data came from the National Health Interview Survey (NHIS - 2015). A total number of 21114 individuals entered the current analysis. The independent variable was years of schooling. The dependent variable was current smoking status. Age, gender, region, marital status, and employment were the covariates. Ethnicity was the moderator. Results: Overall, educational attainment was inversely associated with current smoking. Ethnicity showed a significant interaction with educational attainment suggesting that the protective effects of educational attainment against smoking are smaller for AIANs than for Whites. Conclusion: In the United States, while educational attainment helps individuals stay healthy by avoiding high risk behaviors such as smoking, this effect is smaller for AIANs than Whites, resulting in additional risk of smoking in highly educated AIANs. To reduce ethnic disparities in tobacco use, it is important to go beyond SES inequalities and investigate why high SES ethnic minorities remain at high risk of tobacco use.
Burden of Liver Diseases: A Review from Iran
Authors: Anushiravani Amir , Ghajarieh Sepanlou Sadaf
Published: November 2019

There has been an increase in the burden of liver diseases in Iran, with an increasing trend from communicable to non-communicable diseases. Almost 5400 deaths were due to chronic liver diseases in 2017. We aim to provide a concise update on the epidemiological trends of liver diseases in Iran. Estimations of deaths, disability-adjusted life years, prevalence of chronic liver diseases and cirrhosis in Iran with its common etiologies have been reported. We investigated the major causes of chronic liver diseases in Iran, we have reported our hepatology research centers, and also we have depicted the future of liver diseases in Iran. In 2017, there was a rising trend in chronic liver diseases in Iran. The most common etiologies for chronic liver disease were chronic hepatitis B, chronic hepatitis C, and non-alcoholic steatohepatitis with highest mortalities due to liver cancer and hepatitis C. The prevalence of HBV infection has decreased from 2.9% to 1.3% with effective vaccination, but new cases are still seen due to perinatal transmission. Treatment of HCV has dramatically changed with new drugs which are being produced by local pharmaceuticals at a low cost. The main obstacle in its elimination is finding patients and linkage to care. More than a third of our population have non-alcoholic fatty liver disease in which central obesity had a stronger association than weight itself. Iran has a high burden of liver diseases. The Ministry of Health has effectively controlled hepatitis B and is working towards the World Health WHO’s goals for hepatitis C by 2030. This being said, non-alcoholic fatty liver disease is becoming a major threat to our nation’s health and quality of life.
Predictive Factors of Advanced Colonic Adenomas and Cancer Using Data Mining
Authors: Fatemian Atieh Sadat , Abdolvand Neda , Salimzadeh Hamideh , Delavari Alireza
Published: November 2019

BACKGROUND Colorectal cancer is the third common cancer in Iran. In this study we aimed to identify factors associated with the prevalence of advanced colonic neoplasms among a high-risk population. METHODS Participants were 474 first degree relatives of patients with colon cancer who underwent a screening colonoscopy at Digestive Disease Research Institute, Shariati Hospital affiliated to Tehran University of Medical Sciences. Features examined in this study were age, sex, body mass index, Aspirin use, smoking, and relationship type with patients with cancer in family. Also, patient’s age at the time of cancer diagnosis, number and sex of the patients with colon cancer in the family were assessed. Data analysis was performed by data mining methods using K-Medoid clustering and decision tree C4.5. RESULTS Results showed that female sex of the patients with colon cancer and their young age (< 60 years old) at the time of cancer diagnosis were important predictive factors for the prevalence of colorectal advanced neoplasms among their family members. CONCLUSION Data mining methods were found to be applicable in recognizing predictive factors of colorectal advanced neoplasms in each cluster and tree.
Vitamin D Increases CTLA-4 Gene Expression in Patients with Mild to Moderate Ulcerative Colitis
Authors: Sharifi Amrollah , Vahedi Homayoon , Honarvar Mohammad Reza , Alipoor Behnam , Nikniaz Zeinab , Rafiei Hossein , Hosseinzadeh-Attar Mohammad Javad
Published: November 2019

BACKGROUND Ulcerative colitis (UC) is a chronic inflammatory disorder of the large intestine. Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) is a member of the immunoglobulin superfamily, which binds B7-1 and B7-2 on APCs (antigen-presenting cells), and induces APCs to produce an inhibitory signal to T cells. The aim of this study was to investigate the effect of vitamin D on CTLA-4 gene expression in whole blood samples of patients with UC. METHODS 90 patients with mild to moderate UC were randomized to receive either a single injection of 7.5 mg vitamin D3 or 1 mL normal saline. 90 days following the intervention fold changes in CTLA-4 mRNA expression were determined and statistical comparisons between the two groups were performed. RESULTS Serum vitamin D increased significantly only in the vitamin D group. CTLA-4 fold changes were significantly higher in the vitamin D group compared with the placebo group (median ± IQR: 1.21 ± 2.3 vs. 1.00 ± 1.5, respectively; p = 0.007). CONCLUSION The results of this study revealed that vitamin D administration in patients with UC enhances the CTLA-4 gene expression.
Consequences of Stenting and Endoscopic Papillary Balloon Dilatation in Treatment of Large and Multiple Common Bile Duct Stones
Authors: Hormati Ahmad , Ghadir Mohammad Reza , Hasanpour Dehkordi Ali , Yadollahi Farshad , Salehitali Shahriar , Afifian Mahboobeh
Published: November 2019

BACKGROUND Although stenting for the treatment of large and multiple common bile duct stones has been acceptable to everyone, its efficacy and outcome have not been studied in comparison with other endoscopic procedures. The purpose of this study was to compare the consequences of stenting and endoscopic papilla balloon dilatation for the treatment of large and multiple common bile duct stones. METHODS In a double-blind clinical trial, of 431 patients with bile duct stones referred to the treatment center, 64 patients with multiple common bile duct stones ( ≥ 3) and more than 15 cm were selected for the study, then by random allocation rule the participants were allocated in two groups. They were entered into two different endoscopic papillary balloon dilatation (EPBD) and common bile ducts stenting treatments so that both procedures were performed by a person. Both groups were assessed from the point of views therapeutic outcomes such as duct cleaning, pancreatitis, isolated pain, and duct rupture. Data were collected by a self-made questionnaire that was used before and after the procedure to obtain the needed information. Then data were analyzed using SPSS software version 22 and descriptive and analytical tests were used as appropriated. RESULTS Although the duct cleaning and the complete removal of the stones in the stenting treatment procedure was 93.8%, and in EPBD was 78.3%, no significant difference was observed between the two groups (p = 0.14). Pancreatitis significantly increased after the first and second endoscopic retrograde cholangiopancreatography (ERCP) in the stent group compared with EPBD (p = 0.02). Also, the most frequent cases of isolated pain were in the endoscopic group EPBD (p = 0.0). However, the occurrence of perforation after first ERCP and EPBD was zero, but in the second stage of ERCP, 3.3% of the patients had perforations (p = 0.99). The results indicated that the shape of the stone (circular and angled) was not effective in the result of treatment in the two groups. CONCLUSION The results of this study indicated that in case of experience and skill in conducting the ERCP, common bile duct stenting is still the first line of treatment for large and multiple stones of the common bile ducts.
Comparison of Laughter Yoga and Anti-Anxiety Medication on Anxiety and Gastrointestinal Symptoms of Patients with Irritable Bowel Syndrome
Authors: Tavakoli Tahmine , Davoodi Navid , Jafar Tabatabaee Toktam Sadat , Rostami Zeinab , Mollaei Homa , Salmani Fatemeh , Ayati Sara , Tabrizi Sanaz
Published: November 2019

BACKGROUND Irritable bowel syndrome (IBS) is the most common chronic gastrointestinal (GI) disorder. Patients with IBS usually suffer from anxiety and depression. A combination of psychological approaches and pharmacological treatments can be a significantly effective treatment for IBS. The main objective of the present study was to provide a therapeutic plan based on laughter yoga and anti-anxiety medication, employed for the very first time, and to determine the effectiveness of these treatments on the anxiety and GI symptoms of patients with IBS. METHODS In this randomized, controlled, clinical trial, the participants were 60 patients selected from those who referred to the GI clinic of Vali-asr Hospital (Birjand, Iran) during the study period (April 2017 to March 2017) and were diagnosed as having IBS based on ROME III criteria. The participants were randomly assigned to either the laughter yoga group, the anti-anxiety medication group, or the symptomatic treatment (control) group. Severity levels of anxiety and GI symptoms before and after intervention were determined and compared among these three groups according to approved protocols. RESULTS The severity of IBS symptoms after the interventions was more greatly reduced in the laughter yoga group than in the anti-anxiety medication and control groups (p = 0.006). The severity of anxiety after interventions decreased in all three groups, especially in the yoga treatment group, but the difference was not statistically significant (p = 0.1). CONCLUSION Laughter yoga is more effective than anti-anxiety medication in reducing the GI symptoms of patients with IBS. Therefore, applying laughter yoga along with common pharmacological therapies for patients with IBS might be strongly advised.
A Rare Cause of Gastrointestinal Bleeding in a 65-Year-Old Man with History of Polycythemia Vera
Authors: Aletaha Najmeh , Hamid Hoda , Ayoobi Yazdi Niloofar , Taslimi Reza , Shahbazkhani Bijan , Ketabi Moghadam Pardis
Published: November 2019

Polycythemia vera (PV) is classified as a myeloproliferative disorder (MPD). Such patients are prone to both thrombotic and hemorrhagic events. Although gastrointestinal (GI) bleeding is not a prominent manifestation of PV, it would be life threatening and necessitating hospital admission and blood transfusion if it occurs. GI hemorrhage in these patients may be due to Aspirin usage, peptic ulcer disease (PUD), acquired Von Willbrand disease, Dieulafoy lesion (DL), Mallory Weiss tear, and esophageal and gastric varices. DL is an enlarged, thick-walled artery in the muscularis mucosa with a small submucosal defect. In this case report, we describe a 65-year-old man with history of PV presented with a massive upper GI bleeding. After a therapeutic endoscopic hemostasis failure and reoccurrence of bleeding during hospital admission, an abdominal computed tomography (CT) was ordered, which revealed an aberrant artery originated from aorta directly into the stomach. An angiographic embolization was considered for the patient, which was successfully performed. Our patient was complicated by splenic infarction due to splenic collateral arteries embolization and the overwhelming thrombotic tendency of the patient himself due to the history of PV. Fortunately, our patient’s signs and symptoms responded to supportive therapies and eventually he discharged well.
Celiac Crisis in a Young Woman: Raising Awareness of a Life-Threatening Condition
Authors: Jameshorani Maryam , Pourshams Akram , Sadeghi Anahita , Saffar Hiva , Malekzadeh Reza
Published: November 2019

Celiac crisis is a rare, acute, and life-threatening presentation of celiac disease. Its clinical presentations consist of severe watery non-bloody diarrhea, electrolyte disturbances (i.e. hypokalemia, hyponatremia, hypomagnesemia, hypocalcemia, and metabolic acidosis), hypoproteinemia, and dehydration. Here we present a 33-year-old woman who referred with profuse diarrhea, weight loss, hemodynamic instability, hypokalemia, hypoproteinemia, ascites, pancytopenia, and iron deficiency anemia. She used herbal medicines for constipation and had severe weakness after her childbirth. The patient was diagnosed as having celiac disease through pathological and serological evaluations 10 months earlier. Diagnosis of celiac crisis after ruling out the other causes of resistant celiac was made and she was treated with steroids.
Experimental Validation of Solar Photovoltaic-Based Unified Power Quality Conditioner with Modified Power Angle Control Scheme
Authors: Gowrishankar A. , Ramasamy M.
Published: November 2019

This article proposes a modified Power Angle Control (PAC) strategy for a Unified Power Quality Conditioner (UPQC) with Distributed Generation (DG) for the viable interconnection of a Solar Photovoltaic (SPV) system into the network. The UPQC is composed of both shunt and series Active Power Filters (APFs). Shunt and series APFs are the standout among the best custom power devices, which gives compensation to current- and voltage-based unsettling influences distinctly. In the SPV-interconnected UPQC, the shunt APF supplies power from DG to the associated load, apart from providing a reactive power request, which prompts an expansion in the Volt-Ampere (VA) burden after its rating. The PAC scheme intends the successful use of series and shunt APFs through the sharing of reactive power to decrease the VA burden on shunt APF. The PAC strategy depends on an enhanced Synchronous Reference Frame that is equipped with a Second-Order Generalized Integrator–based Phase Locked Loop, which has simple calculations, is vibrant, and utilizes existing estimations of the SPV-incorporated UPQC. The execution of the proposed SPV-incorporated UPQC is examined with the presence of nonlinear and reactive burdens. The dynamic performance of the SPV-UPQC is checked under the grid disturbances, for example, voltage sag, swell, varying load, and change in sunlight-based light. The viability of the proposed control strategy is assessed through the digital simulation and equipment exploratory model results.
Identification ofkit-ligand aas the Gene Responsible for the Medaka Pigment Cell Mutantfew melanophore
Authors: Otsuki Yuji , Okuda Yuki , Naruse Kiyoshi , Saya Hideyuki
Published: November 2019

The body coloration of animals is due to pigment cells derived from neural crest cells, which are multipotent and differentiate into diverse cell types. Medaka (Oryzias latipes) possesses four distinct types of pigment cells known as melanophores, xanthophores, iridophores, and leucophores. The few melanophore (fm) mutant of medaka is characterized by reduced numbers of melanophores and leucophores. We here identify kit-ligand a (kitlga) as the gene whose mutation gives rise to the fm phenotype. This identification was confirmed by generation of kitlga knockout medaka and the findings that these fish also manifest reduced numbers of melanophores and leucophores and fail to rescue the fm mutant phenotype. We also found that expression of sox5, pax7a, pax3a, and mitfa genes is down-regulated in both fm and kitlga knockout medaka, implicating c-Kit signaling in regulation of the expression of these genes as well as the encoded transcription factors in pigment cell specification. Our results may provide insight into the pathogenesis of c-Kit–related pigmentation disorders such as piebaldism in humans, and our kitlga knockout medaka may prove useful as a tool for drug screening.
A Genetic Screen To Identify Genes Influencing the Secondary Redox Couple NADPH/NADP+ in the Yeast Saccharomyces cerevisiae
Authors: Yadav Shambhu , Mody Tejasvinee Atul , Sharma Archi , Bachhawat Anand Kumar
Published: November 2019

NADPH is an important cofactor in the cell. In addition to its role in the biosynthesis of critical metabolites, it plays crucial roles in the regeneration of the reduced forms of glutathione, thioredoxins and peroxiredoxins. The enzymes and pathways that regulate NADPH are thus extremely important to understand, and yet are only partially understood. We have been interested in understanding how NADPH fluxes are altered in the cell. We describe here both an assay and a genetic screen that allows one to discern changes in NADPH levels. The screen exploits the secondary redox property of NADPH. At low levels of glutathione we show that the redox contributions of NADPH become critical for growth, and we have used this to develop a genetic screen for genes affecting NADPH homeostasis. The screen was validated in pathways that both directly (pentose phosphate pathway) and indirectly (glycolytic pathway) affect NADPH levels, and was then exploited to identify mitochondrial genes that affect NADPH homeostasis. A total of 239 mitochondrial gene knockouts were assayed using this screen. Among these, several genes were predicted to play a role in NADPH homeostasis. This included several new genes of unknown function, and others of poorly defined function. We examined two of these genes, FMP40 which encodes a protein required during oxidative stress and GOR1, glyoxylate reductase. Our studies throw new light on these proteins that appear to be major consumers of NADPH in the cell. The genetic screen is thus predicted to be an exceedingly useful tool for investigating NADPH homeostasis.
Challenges and Approaches to Genotyping Repetitive DNA
Authors: Morton Elizabeth A , Hall Ashley N , Kwan Elizabeth , Mok Calvin , Queitsch Konstantin , Nandakumar Vivek , Stamatoyannopoulos John , Brewer Bonita J , Waterston Robert , Queitsch Christine
Published: November 2019

Individuals within a species can exhibit vast variation in copy number of repetitive DNA elements. This variation may contribute to complex traits such as lifespan and disease, yet it is only infrequently considered in genotype-phenotype associations. Although the possible importance of copy number variation is widely recognized, accurate copy number quantification remains challenging. Here, we assess the technical reproducibility of several major methods for copy number estimation as they apply to the large repetitive ribosomal DNA array (rDNA). rDNA encodes the ribosomal RNAs and exists as a tandem gene array in all eukaryotes. Repeat units of rDNA are kilobases in size, often with several hundred units comprising the array, making rDNA particularly intractable to common quantification techniques. We evaluate pulsed-field gel electrophoresis, droplet digital PCR, and Nextera-based whole genome sequencing as approaches to copy number estimation, comparing techniques across model organisms and spanning wide ranges of copy numbers. Nextera-based whole genome sequencing, though commonly used in recent literature, produced high error. We explore possible causes for this error and provide recommendations for best practices in rDNA copy number estimation. We present a resource of high-confidence rDNA copy number estimates for a set of S. cerevisiae and C. elegans strains for future use. We furthermore explore the possibility for FISH-based copy number estimation, an alternative that could potentially characterize copy number on a cellular level.
Nonsynonymous Mutations in Linker-2 of the Pdr5 Multidrug Transporter Identify a New RNA Stability Element
Authors: Rahman Hadiar , Rudrow Andrew , Carneglia Joshua , Joly Sister Stephen Patrick , Nicotera Dante , Naldrett Michael , Choy John , Ambudkar Suresh V , Golin John
Published: November 2019

Analysis of synonymous mutations established that although the primary amino acid sequence remains unchanged, alterations in transcription and translation can result in significant phenotypic consequences. We report the novel observation that a series of nonsynonymous mutations in an unconserved stretch of amino acids found in the yeast multidrug efflux pump Pdr5 increases expression, thus enhancing multidrug resistance. Cycloheximide chase experiments ruled out the possibility that the increased steady-state level of Pdr5 was caused by increased protein stability. Quantitative-RT PCR experiments demonstrated that the mutants had levels of PDR5 transcript that were two to three times as high as in the isogenic wild-type strain. Further experiments employing metabolic labeling of mRNA with 4-thiouracil followed by uracil chasing showed that the half-life of PDR5 transcripts was specifically increased in these mutants. Our data demonstrate that the nucleotides encoding unconserved amino acids may be used to regulate expression and suggest that Pdr5 has a newly discovered RNA stability element within its coding region.
Pollen morphology and availability of pollen and stigma in Syzygium (Myrtaceae)
Authors: Nacata Guilherme , Belonsi Talita Kely , Gasparino Eduardo Custódio , Andrade Renata Aparecida de
Published: November 2019

Abstract The present study aimed to describe the pollen morphology in three species of Syzygium, to test pollen availability and stigma receptivity. The pollen was analyzed by light and scanning microscopy to show the detailed ornamentation of the pollen grains. Pollen availability was measured according to two protocols. Stigmatic receptivity was tested at different stages of flower development. There are differences among the pollen morphology for the species studied; when the polar and equatorial diameters are measured in the equatorial view of the pollen grains, it is observed that S. jambos is the largest species concerning the two values of the diameters, S. malaccense is the smallest species and S. aqueum, the largest species in relation to the polar diameter in the equatorial view. For the equatorial diameter in equatorial view, S. aqueum showed the smallest diameters and S. malaccense, the major diameters, but the two species overlap in the values of the measured diameters. There are variations in the number of apertures in the pollen grains. Alexander’s stain is more sensitive to estimate pollen availability and the species S. aqueum and S. jambos have the same schedules of pollen availability and stigma receptivity.
Análise comparativa dos efeitos do mel, do óleo-serina de copaíba e de um produto comercial (fibrinolisina, desoxirribonuclease e cloranfenicol) na cicatrização por segunda intenção, em ratos.
Authors: Biondo-Simões Maria de Lourdes Pessole , Henning Júnior Lucélio , Boen Bruno Russiano de Oliveira , Prado Julyana Leoni do , Costa Luiza Rodrigues da , Robes Rogério Ribeiro , Ioshii Sérgio Ossamu
Published: November 2019

RESUMO Objetivo: comparar a cicatrização, por segunda intenção, sob os efeitos da aplicação tópica de mel, óleo-resina de copaíba e um produto comercial (fibrinolisina, desoxirribonuclease e cloranfenicol) a um grupo controle, em ratos. Métodos: ressecção de pele, com 1cm de diâmetro, foi realizada no dorso de 40 ratos alocados em quatro grupos de dez animais. Todas as feridas foram limpas, diariamente, com 2ml de solução de NaCl 0,9%. O primeiro grupo (controle - GC) ficou restrito a tal procedimento. Nas feridas do segundo (GM), terceiro (GO) e quarto grupos (GF), após limpeza, aplicou-se, respectivamente, 1ml de mel, 1ml de óleo-resina de copaíba e 1ml de creme contendo fibrinolisina, desoxirribonuclease e cloranfenicol. Ocluíram-se as feridas com gaze estéril. Imediatamente após a incisão e nos dias três, sete e 14 do experimento, as feridas foram copiadas e, usando planimetria, analisou-se a contração. Após a eutanásia, a histologia foi utilizada para avaliação da reação inflamatória e do colágeno nas cicatrizes. Resultados: a redução da área da ferida do GM (p=0,003), GO (p=0,011) e GF (p=0,002) foram superiores ao do GC. A quantidade de colágeno tipo I presente no GM e no GO foi superior aos grupos GC e GF (p<0,05). Houve predominância do estágio inflamatório crônico no GM (p=0,004), GO (p<0,001) e GF (p=0,003) quando comparados ao GC. Conclusão: o uso tópico do mel e do óleo-resina de copaíba aumenta a contração da ferida, a presença de colágeno tipo I e acelera a cicatrização.
Pré-habilitação: como preparar nossos pacientes para cirurgias abdominais eletivas de maior porte?
Authors: Gonçalves Carolina Gomes , Groth Anne Karoline
Published: November 2019

RESUMO Várias doenças da cavidade abdominal têm, na abordagem cirúrgica, sua principal forma de tratamento. Entretanto, o próprio procedimento cirúrgico é um agente estressor que pode promover efeitos adversos não relacionados com o objetivo do tratamento. A pré-habilitação emergiu como um programa multifatorial de condicionamento de saúde pré-operatório, que promove melhora na capacidade funcional e na evolução pós-operatória. O presente estudo faz uma revisão da literatura usando os bancos de dado MEDLINE, Ovid, Google Scholar e Cochrane para determinar o conceito, as indicações, os meios de seleção dos pacientes, e para sugerir as formas de implementação do programa de pré-habilitação em cirurgias abdominais de grande porte.
Excisão total de mesorreto transanal combinada à dissecção laparoscópica: resultados perioperatórios e avaliação da qualidade do mesorreto
Authors: Soares Marllus Braga , Pitombo Marcos Bettini , Pupo Neto João de Aguiar , Galvão Isis Alves
Published: November 2019

RESUMO Objetivo: avaliar os resultados perioperatórios e pós-operatórios relacionados à excisão total do mesorreto transanal, bem como, qualidade dos espécimes obtidos pela excisão total do mesorreto transanal através da análise histopatológica da qualidade do mesorreto, acometimento das margens proximal e distal e positividade da margem circunferencial. Métodos: estudo prospectivo, descritivo, em pacientes consecutivos portadores de adenocarcinoma de reto localizados até 10cm da borda anal, sem metástase à distância, submetidos ou não à quimioterapia e radioterapia neoadjuvantes. Foram avaliados a presença de dificuldades técnicas, índice de conversão para via abdominal, tempo cirúrgico e intercorrências intra e pós-operatórias. A qualidade do mesorreto foi classificada em ressecção completa, parcialmente completa ou incompleta. Resultados: entre dezembro de 2016 e maio de 2019, 41 pacientes foram submetidos à excisão total do mesorreto transanal, dos quais 75% foram classificados como estágio clínico III, 13% estágio clínico II e 12% estágio clínico I. A média de distância entre borda anal e borda inferior do tumor foi de 6,2cm. Quarenta por cento dos tumores encontravam-se na parede retal anterior e 17% foram classificados como circunferenciais. A média de tempo operatório foi de 189 minutos. A média de internação hospitalar foi de 4,6 dias. Não houve óbitos intra-hospitalares. Oitenta e dois por cento dos espécimes foram classificados como ressecção completa. Conclusão: a excisão total do mesorreto transanal demonstra adequada qualidade do mesorreto e adequadas margens cirúrgicas, estando associada a baixos índices de complicações perioperatórias, tempo cirúrgico aceitável e curto tempo de hospitalização.
Natural abundance of 15N of N derived from the atmosphere by different strains of Bradyrhizobium in symbiosis with soybean plants
Authors: Araujo Karla Emanuelle Campos , Torres Júnior Carlos Vergara , Guimarães Ana Paula , Silva Mara Alexandre da , Alves Bruno José Rodrigues , Urquiaga Segundo , Boddey Robert Michael
Published: November 2019

ABSTRACT: To quantify the BNF contribution to legumes using the 15N natural abundance technique, it is important to know the abundance of 15N of the plants grown entirely dependent on BNF (value ‘B’). The aim of the study was to determine the 15N natural abundance of N2 fixed by different Bradyrhizobium strains in symbiosis with one soybean cultivar. Treatments consisted of soybean plants cultivated with and without inoculation with ten Bradyrhizobium strains, in five replicates planted in Leonard jars in a sand/vermiculite mixture. Plants were harvested after 46 days. The ‘B’ values of the aerial tissue (‘Bs’) ranged from -2.6 to -3.9 ‰. There was a tendency for the ‘Bs’ values of plants inoculated with strains of B. elkanii to be more negative than plants inoculated with other strains. All ‘B’ values of the whole plant were less than 1 unit of δ15N (‰) different from zero, suggesting that the symbioses have little tendency to show significant isotopic fractionation during N2 fixation, but there is considerable depletion in 15N of the N translocated to the shoot tissue.
Accumulation of organic solutes and enzymatic activity in cut roses (Rosaceae) cultivated with physiological effect products in the Sub- Middle São Francisco River Valley
Authors: Silva Maria de Lourdes Neres da , Santos Mariana Correia , Barbosa Mayara Suzanne de Melo , Sabino João Henrique Ferreira , Ribeiro Hugo Leonardo Coelho , Ramos Anamaria Ribeiro Pereira , Silva Marcelle Almeida da , Beckmann-Cavalcante Márkilla Zunete
Published: November 2019

ABSTRACT: The aim of the present study was to evaluate the accumulation of carbohydrates, protein, and proline as well as the activity of the antioxidant enzymes superoxide dismutase and catalase in the Ambiance cultivar of cut rose plants grown with the application of physiological effect products in the Sub-Middle São Francisco River Valley in Brazil. The experiment was performed under a mesh screen with 50% shading. The experimental design used randomized blocks with four repetitions and six treatments: T1) control (water); T2) boscalid; T3) pyraclostrobin; T4) boscalid + pyraclostrobin (T2 + T3); T5) fluxapyroxad + pyraclostrobin; T6) plant growth regulators 4-(indol-3-yl) butyric acid (IBA) + gibberellic acid (GA3) + kinetin; these treatments were applied every 15 days throughout the crop cycle. To determine the accumulation of solutes and enzymatic activity, 8 leaves was collected every 48 h. Leaves were immediately immersed in liquid nitrogen and frozen until further analysis in the laboratory. Results showed that the product combinations boscalid + pyraclostrobin and fluxapyroxad + pyraclostrobin as well as the plant growth regulators were the treatments with the most consistent responses throughout the evaluated cycle, providing a greater accumulation of solutes in rose leaves, as an osmotic adjustment strategy against stress from high temperatures, particularly when proline accumulation is observed. With regard to enzymatic activity, plant regulators showed more consistent results when compared with other treatments, increasing both superoxide dismutase and catalase activity. The marked accumulation of organic solutes and the high enzymatic activity, particularly of catalase, indicated that rose plants use such mechanisms as a defense against the region’s high temperatures.
Induction of lactation: an economic study of tool for dairy heifers with successive reproductive failures
Authors: Maffi Andressa Stein , Luz Gabriela Bueno , Gasperin Bernardo Garziera , Mattos Raine Fonseca , Xavier Eduardo Gularte , Corrêa Marcio Nunes , Brauner Cássio Cassal
Published: November 2019

ABSTRACT: The aim of this study was to evaluate the economic viability of the use of a lactation induction protocol in Holstein heifers. Were collected data of nutritional, reproductive and sanitary management pre and post-lactation period from 30 induction heifers (IG) and 30 pregnant heifers (CG). All animals were inseminated around 14 months of age and started lactation at 23±4 months. The animals of IG were heifers do not pregnant after two inseminations and natural service e were induced to lactation according to protocol: from the 1st to the 8th day, 30 mg of estradiol benzoate were administered daily, together with 300 mg of progesterone. From the 9th until the 14th, animals only received daily doses of 20 mg estradiol benzoate. On 16th day, 0.56 mg of sodium cloprostenol was administered and injections of 40 mg of dexamethasone sodium phosphate were administered daily from the 19th to the 21st day. On the 1st, 8th, 15th and 22nd day, the animals received a dose of sometribove zinc. The animals of CG were managed according to the farm routine. The costs of the CG during pre and post lactation (nutrition, sanitary, reproduction management) e were higher than the IG, however, when evaluating the economic viability due to lower milk production, the induced group did not leave profit in the first year of use. When comparing the lactation induction with the sale of animals that did not conceive and purchase of animals for replacement, this last scenario showed itself as a better alternative.
Photogrammetry and 3D prototyping: A low-cost resource for training in veterinary orthopedics
Authors: Lima Luiz Felipe Souza de , Barros Anna Júlia Brandão Paes de , Martini Andresa de Cássia , Stocco Matias Bassinello , Kuczmarski Antonio Henrique , Souza Roberto Lopes de
Published: November 2019

ABSTRACT: Rapid prototyping (RP) is an innovative technology that allows one to obtain a prototype of a mold quickly and accurately from a virtual model. This study aimed to establish the use of photogrammetry and 3D prototyping for the production of bone biomodels of the canine species for training in orthopedic techniques in veterinary medicine. Virtual bio-modelling was performed by the photogrammetry technique with commercial anatomical pieces, and physical biomodelling was performed by 3D printing. Osteotomies were performed on the biomodels that served as platforms for osteosynthesis of the femur and ileum, and the final product was not associated with a risk of biological contamination, was able to support special orthopedic materials, and was used for training and surgical planning. We concluded that the use of photogrammetry and RP for the production of bone biomodels of the canine species enabled techniques for fracture reduction to be performed with the use of special instruments, enabling training in the area of veterinary orthopedics in an economically viable manner with an alternative to experimental animals.
Seroprevalence of Leptospira spp. infection in sheep from northwest of Rio Grande do Sul, Brazil
Authors: Consalter Angélica , Zanella Eraldo Lourenso , Martins Gabriel Mendes de Souza , Araujo Hugo Libonati de , Lilenbaum Walter , Souza Guilherme Nunes de , Ferreira Ana Maria Reis
Published: November 2019

ABSTRACT: Leptospirosis is a zoonosis that is widely distributed in tropical countries. This infection is also associated with reproductive losses in livestock, which has a significant economic impact. The objective of this study is to investigate the seroprevalence and risk factors associated with leptospiral infection in commercial sheep flocks from the northwestern mesoregion of Rio Grande do Sul. Serum samples were analyzed in 319 sheep using the Microscopic Agglutination Test (MAT). At the time of sampling, an individual and herd questionnaire was carried out to investigate the risk factors. On an individual level, positive reactions were observed in 5.6% (18/319) of the studied sera, with Sejroe being the most prevalent serogroup. Animals >3 years old were more likely to be seropositive than animals 1-3 years old (OR 14.4; 95% CI 1.9-110). The predominance of Sejroe serogroup in this manner draws attention to how subclinical infection is associated with economic losses. Therefore, the importance of maintaining measures for the prevention and control of leptospirosis among the sheep flocks of the studied region is reiterated.
Oxidative stability, fatty acid composition and health lipid indices of Longissimus dorsi muscle from Aberdeen Angus steers produced in different feeding systems
Authors: Terevinto Alejandra , Cabrera María Cristina , Saadoun Ali
Published: November 2019

ABSTRACT: The aim of this study was to evaluate the oxidative and anti-oxidative status of unaged and aged Longissimus dorsi (LD) muscle obtained from a commercial abattoir. Also, fatty acid composition and calculated health lipid indices of meat were compared. Animals slaughtered were Aberdeen Angus steers raised in three typical feeding systems of Uruguay: (1) Pasture (n=10), (2) Pasture + Supplement (P+S) with corn grain (n=10), and (3) Feedlot (n=10). Feeding had no effect on lipid and protein oxidation but the antioxidant enzymes activities were affected as follows: superoxide dismutase (SOD): Pasture, Feedlot > P+S; catalase: Pasture > P+S, Feedlot; glutathione peroxidase (GPx): Feedlot > P+S > Pasture. Total antioxidant potential, determined by Fenton reaction, was higher in aged meat from Pasture compared with aged meat from Feedlot. Meat from Pasture and P+S presented a higher eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and total n-3 content and a lower lipid content, n-6/n-3 ratio and trombogenic index compared to meat from Feedlot. In conclusion, Pasture seems to produce a more resistant meat against induced oxidation processes, particularly after ageing, and a healthier meat together with meat from P+S system, regarding fatty acid composition and lipid indices, compared to Feedlot.
MEMÓRIAS DO LIXO: LUTA E RESISTÊNCIA NAS TRAJETÓRIAS DE CATADORES DE MATERIAIS RECICLÁVEIS DA ASMARE1
Authors: Sousa Romário Rocha , Pereira Rafael Diogo , Calbino Daniel
Published: November 2019

RESUMO O presente artigo teve por objetivo analisar o processo de luta e de resistência vivenciado por catadores de materiais recicláveis, vinculados à Associação dos Catadores de Papel, Papelão e Material Reaproveitável (ASMARE), localizada em Belo Horizonte (MG). Em termos metodológicos, adotou-se a História Oral e a Análise do Discurso, buscando analisar trajetórias de vida de associados da ASMARE. Os principais resultados apontam que o trabalho dos catadores ocupa um lugar de contradição na cadeia da reciclagem. Por um lado, existe a geração de renda e a produção de sentidos, que permitem a ressignificação do trabalho dos catadores como agentes ambientais, por outro, as condições em que se inserem ainda são precárias e de invisibilidade social. A árdua ocupação com o trabalho da reciclagem cria o desafio de os catadores lidarem com a constante ambivalência de sua condição, em que o trabalho ressignificado ainda disputa espaço com o preconceito e com a exclusão social.
THE PEOPLE’S BOUROUGH PLAN OF ACTION: A COUNTER-PROJECT OF INSURGENT CITIZENSHIP1
Authors: Oliveira Clarice de , Alberti Camila Bellaver , Silva Laura Boeck , Nodari Gabriela Rosa
Published: November 2019

ABSTRACT Porto Alegre is divided in eight Boroughs of Planning. Each Borough has a representative in the Urban Planning City Council (UPCC). In 2018 social movements organized to conquer this. From this, some of the Boroughs’ councilors felt the need to better inform themselves on the terms and subjects discussed in the UPCC, and to ensure that their local issues would be discussed. For that manner, the People’s Borough Plan of Action (PBPA) project was created by a coalition of social movements, the architect’s association (IAB-RS) and the university to perform counter-hegemonic actions. The project is based on the insurgent planning theory, which understands urban development from the standpoint of the global south as being essentially performed by communities, activists and grassroots strategies. Thus, the project moves across both invited and invented spaces of action in a non-binary relationship, with the aim of providing the grassroots movements of insurgent citizenship with technical assistance to support their claims and desires over the city they live. Regarding the City’s Master Plan revision, the PBAP represents a counter-plan related to the creation of differential spaces. Therefore, they create a moment of realization of the right to the city.