bluuebunny/crossref_metadata_2025 huggingface dataset:
Dynamic ECG signal quality evaluation based on persistent homology and GoogLeNet method
Authors: Ren Yonglian , Liu Feifei , Xia Shengxiang , Shi Shuhua , Chen Lei , Wang Ziyu
Published: March 2023

Cardiovascular disease is a serious health problem. Continuous Electrocardiograph (ECG) monitoring plays a vital role in the early detection of cardiovascular disease. As the Internet of Things technology continues to mature, wearable ECG signal monitors have been widely used. However, dynamic ECG signals are extremely susceptible to contamination. Therefore, it is necessary to evaluate the quality of wearable dynamic ECG signals. The topological data analysis method (TDA) with persistent homology, which can effectively capture the topological information of high-dimensional data space, has been widely studied. In this study, a brand-new quality assessment method of wearable dynamic ECG signals was proposed based on the TDA with persistent homology method. The point cloud of an ECG signal was constructed, and then the complex sequence was generated and displayed as a persistent barcode. Finally, GoogLeNet based on the transfer learning model with a 10-fold cross-validation method was used to train the classification model. A total of 12-leads ECGs Dataset and single-lead ECGs Dataset, established based on the 2011 PhysioNet/CinC challenge dataset, were both used to verify the performance of this method. In the study, 773 “acceptable” and 225 “unacceptable” signals were used as 12-leads ECGs Dataset. We relabeled 12,000 ECG signals in the challenge dataset, and treated them as single-lead ECGs Dataset after empty lead detection and balance datasets. Compared with the traditional ECG signal quality assessment method mainly based on waveform characteristics and time-frequency characteristics, the performance of the quality assessment method proposed. In this study, the classification performance of the proposed method are fairly great, mAcc = 98.04%, F1 = 98.40%, Se = 97.15%, Sp = 98.93% for 12-leads ECGs Dataset and mAcc = 98.55%, F1 = 98.62%, Se = 98.37%, Sp = 98.85% for single-lead ECGs Dataset.
Loss of action-related function and connectivity in the blind extrastriate body area
Authors: Yizhar Or , Tal Zohar , Amedi Amir
Published: March 2023

The Extrastriate Body Area (EBA) participates in the visual perception and motor actions of body parts. We recently showed that EBA’s perceptual function develops independently of visual experience, responding to stimuli with body-part information in a supramodal fashion. However, it is still unclear if the EBA similarly maintains its action-related function. Here, we used fMRI to study motor-evoked responses and connectivity patterns in the congenitally blind brain. We found that, unlike the case of perception, EBA does not develop an action-related response without visual experience. In addition, we show that congenital blindness alters EBA’s connectivity profile in a counterintuitive way—functional connectivity with sensorimotor cortices dramatically decreases, whereas connectivity with perception-related visual occipital cortices remains high. To the best of our knowledge, we show for the first time that action-related functions and connectivity in the visual cortex could be contingent on visuomotor experience. We further discuss the role of the EBA within the context of visuomotor control and predictive coding theory.
Nutritional profiling and contaminant levels of five underutilized fish species in Norway
Authors: Kendler Sophie , Thornes Frida Walle , Jakobsen Anita Nordeng , Lerfall Jørgen
Published: March 2023

Exploring and making use of underutilized marine resources can be a sustainable approach to achieve future demands of fish consumption by the ever-growing population. Five species, namely European plaice (Pleuronectes platessa), European flounder (Platichthys flesus), lemon sole (Microstomus kitt), megrim (Lepidorhombus whiffiagonis), and thornback ray (Raja clavate), often captured as by-catch in Norway, were characterized for their nutritional value and potential accumulation of hazardous components. The proximate composition, protein profile, fatty acid profile as well as essential and toxic trace elements and polychlorinated biphenyls (PCBs) were analyzed. Digestible indispensable amino acid (DIAA) ratios and scores (DIAAS) and contributions of omega-3 fatty acids to the diet were calculated. Analysis on proximate composition revealed low fat contents of 0.74 to 1.25% and sufficient protein contents between 16.9 and 24% in the five species. Results of DIAA indicate a profitable distribution, with contributions exceeding the daily intake recommendations for an adult person related to a 200 g fillet. Moreover, findings on the distribution of eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) showed remarkable results, considering that the investigated species are lean fish. All five investigated fish exceed the recommended average daily intake level (AI) of EPA + DHA in a 200 g portion. As to toxic trace elements and PCBs, no significantly elevated levels were found considering a portion size of 200 g. Consequently, the nutritional quality of the investigated fish can be regarded as profitable with overall low potential health risks.
Consumption of very low-mineral water may threaten cardiovascular health by increasing homocysteine in children
Authors: Huang Yujing , Tan Yao , Wang Lingqiao , Lan Lan , Luo Jiaohua , Wang Jia , Zeng Hui , Shu Weiqun
Published: March 2023

Introduction

Homocysteine (Hcy) is a critical factor for cardiovascular injury, and the elevation of Hcy in children will inevitably increase the risk of cardiovascular disease in adulthood. This study explored the effect of very low-mineral water on children’s Hcy and cardiovascular health.

Materials and methods

This was a retrospective cohort study that recruited two groups of 10–13-year-old children who had consumed direct drinking water (DDW) in school for 4 years. The control group (NW) (119 boys, 110 girls) consumed normal DDW (conductivity 345 μs/cm). The very low-mineral water consumption group (VLW) (223 boys, 208 girls) consumed very low-mineral DDW (conductivity 40.0 μs/cm). Serum Hcy, Hcy metabolites, cofactors of Hcy metabolism, and cardiovascular biomarkers were assessed and standardized by age- and sex-specific Z-scores, and the differences between the two groups were analyzed with independent t-test. The relationships between Hcy metabolism biomarkers and key factors, cardiovascular biomarkers, serum Ca, and mineral intake were analyzed with linear regression.

Results

Compared with the NW group, the VLW group had significantly higher serum Hcy, Apo-B, Apo-B/A1, and oxLDL, and lower serum 1,25,(OH)2D3, vitamin B6 and B12, 5-methyltetrahydrofolate, and Apo-A1. Serum Hcy was positively associated with serum Apo-B and Apo-B/A1, and negatively associated with Ca intake from water and serum 1,25,(OH)2D3.

Conclusion

This study suggested that drinking very low-mineral water may increase Hcy level and oxidative stress, worsen lipid profile, and threaten the cardiovascular system in children. Reducing 1,25,(OH)2D3, and disordering of calcium metabolism might play important roles. This study first established an association between demineralized drinking water and cardiovascular health in children, suggesting a new environmental concern risk to cardiovascular health.
Genetic analyses of DNA repair pathway associated genes implicate new candidate cancer predisposing genes in ancestrally defined ovarian cancer cases
Authors: Alenezi Wejdan M. , Fierheller Caitlin T. , Serruya Corinne , Revil Timothée , Oros Kathleen K. , Subramanian Deepak N. , Bruce Jeffrey , Spiegelman Dan , Pugh Trevor , Campbell Ian G. , Mes-Masson Anne-Marie , Provencher Diane , Foulkes William D. , Haffaf Zaki El , Rouleau Guy , Bouchard Luigi , Greenwood Celia M. T. , Ragoussis Jiannis , Tonin Patricia N.
Published: March 2023

Not all familial ovarian cancer (OC) cases are explained by pathogenic germline variants in known risk genes. A candidate gene approach involving DNA repair pathway genes was applied to identify rare recurring pathogenic variants in familial OC cases not associated with known OC risk genes from a population exhibiting genetic drift. Whole exome sequencing (WES) data of 15 OC cases from 13 families tested negative for pathogenic variants in known OC risk genes were investigated for candidate variants in 468 DNA repair pathway genes. Filtering and prioritization criteria were applied to WES data to select top candidates for further analyses. Candidates were genotyped in ancestry defined study groups of 214 familial and 998 sporadic OC or breast cancer (BC) cases and 1025 population-matched controls and screened for additional carriers in 605 population-matched OC cases. The candidate genes were also analyzed in WES data from 937 familial or sporadic OC cases of diverse ancestries. Top candidate variants in ERCC5, EXO1, FANCC, NEIL1 and NTHL1 were identified in 5/13 (39%) OC families. Collectively, candidate variants were identified in 7/435 (1.6%) sporadic OC cases and 1/566 (0.2%) sporadic BC cases versus 1/1025 (0.1%) controls. Additional carriers were identified in 6/605 (0.9%) OC cases. Tumour DNA from ERCC5, NEIL1 and NTHL1 variant carriers exhibited loss of the wild-type allele. Carriers of various candidate variants in these genes were identified in 31/937 (3.3%) OC cases of diverse ancestries versus 0-0.004% in cancer-free controls. The strategy of applying a candidate gene approach in a population exhibiting genetic drift identified new candidate OC predisposition variants in DNA repair pathway genes.
Regional citrate anticoagulation for continuous renal replacement therapy in newborns
Authors: Huang Haixia , Deng Xing , Bai Ke , Liu Chengjun , Xu Feng , Dang Hongxing
Published: March 2023

Background

Regional citrate anticoagulant (RCA) is recommended as the preferred anticoagulant regimen for continuous renal replacement therapy (CRRT) in adults; however, it is rarely reported in neonates due to concerns associated with their immature liver. Few studies have reported on the use of RCA to evaluate the safety and efficacy of RCA-CRRT in neonates.

Method

In this retrospective observational study, we reviewed the clinical records of neonates who underwent RCA-CRRT at our pediatric intensive care unit between September 2015 to January 2021.

Results

A total of 23 neonates underwent 57 sessions of RCA-CRRT. Their mean age was 10.1 ± 6.9 days and mean weight was 3.0 ± 0.7 kg (range, 0.95–4 kg). The mean filter life was 31.54 ± 19.58 h (range, 3.3–72.5 h). Compared to pretreatment values, the total-to-ionized calcium ratio (T/iCa) on RCA-CRRT increased (2.00 ± 34 0.36 vs. 2.19 ± 0.40, P = 0.056) as did the incidence of T/iCa levels >2.5 (11.4 vs. 14.3, P = 0.477), albeit not significantly. Using a post-treatment T/iCa threshold of 2.5, we divided all the cases into citrate accumulation (CA) and non-CA (NCA) groups. Compared with the NCA group, the CA group had significantly higher body weight (3.64 ± 0.32 kg vs. 2.95 ± 0.41 kg, P = 0.033) and significantly lower blood flow rate per body weight ml/kg/min (3.08 ± 0.08 vs. 4.07 ± 0.71, P = 0.027); however, there was no significant difference between the two groups in terms of age, corrected gestational age, the PRISM-III score, and biochemical tests.

Conclusion

RCA-CRRT is safe and effective for neonates. After appropriate adjustments of the RCA-CRRT parameters, the incidence of CA was not higher in neonates than in children or adults, and CA was not found to be significantly correlated with age or corrected gestational age.
Pexidartinib synergize PD-1 antibody through inhibiting treg infiltration by reducing TAM-derived CCL22 in lung adenocarcinoma
Authors: Zhang Wei , Jiang Xi , Zou Youcheng , Yuan Lihua , Wang Xiaobo
Published: March 2023

There is a crosstalk between Tumor-associated macrophages (TAM) and tumor-infiltrating T cells in tumor environment. TAM could inhibit the activity of cytotoxic T cells; TAM could also regulate the composition of T cells in tumor immune environment. The combination therapy for TAM and tumor infiltrated T cells has been widely noticed, but the crosstalk between TAM and tumor infiltrated T cells remains unclear in the process of combination therapy. We treated lung adenocarcinoma tumor models with pexidartinib, which targets macrophage colony stimulating factor receptor (M-CSFR) and c-kit tyrosine kinase, to inhibited TAM. Pexidartinib inhibited the ratio of macrophages in the tumor and also altered macrophage polarization. In addition to reprogram TAM, pexidartinib also changed the composition of tumor-invasive T cells. After pexidartinib treatment, the total number of T cells, CD8+ T cells and Treg cells were all decreased, the ratio of CD8+T/Treg increased significantly. According to the analysis of cytokines and chemokines during the treatment of pexidartinib, CCL22, as a chemokine for Treg recruitment, significantly decreased after the treatment of pexidartinib. Base on the above observation, the combination of pexidartinib and PD-1 antibody were used in the treatment of lung adenocarcinoma subcutaneous tumor model, the combination therapy has significantly improved the efficacy of tumor treatment compared with the monotherapy. Meanwhile, compared with pexidartinib monotherapy, the combination treatment further switches the polarization status of tumor-associated macrophages. In summary, our results showed that the combination of pexidartinib and PD-1 antibody showed a synergy and significantly improved the anti-tumor efficacy, through pexidartinib increasing CD8T/Treg ratio by reducing TAM-derived CCL22.
Traffic safety assessment method of the immersed tunnel based on small target visual recognition image
Authors: Yang Meng , Lu Shanfeng , Ding Hao , Chen Jianzhong
Published: March 2023

The quality of lighting installation performance has a direct impact on the traffic safety of immersed tunnels. To effectively investigate and judge the traffic safety of immersed tunnels having different lighting installations, a traffic safety assessment method for immersed tunnels based on lighting performance degradation was put forward in this study by using big data technology. Numerical simulation was used to simulate the lighting environment in an immersed tunnel under different conditions of lighting performance degradation, conduct the small target recognition test in a physical tunnel, and calculate the traffic safety factor; then, a real-time kinematic assessment model of traffic safety in immersed tunnels was built in combination with the key index factors influencing lighting installations in immersed tunnels. The test results showed that the performance degradation of lighting installations positively correlated with the visual cognition of drivers and passengers. long short-term memory neural network model can effectively assess the traffic safety of immersed tunnels, and the root mean square error (RMSE) and coefficient of determination of the model were separately 1.029 and 0.95, which were superior to the RMSE and coefficient of determination of random forest and recurrent neural network model, and the running time was often less than 1min, complying with the rea; -time assessment requirements; the boundary value of the traffic safety factor of immersed tunnels was 0.6304, and if a value was less than the boundary value, it indicated that the performance of lighting installations was not good and might pose a threat to traffic safety. The research results provided a new perspective for the status assessment of lighting installations in immersed tunnels and also offered a theoretical basis for fine maintenance and repairs of lighting installations.
Failure to improve task performance after visuomotor training with error reduction feedback for young adults
Authors: Lin Yen-Ting , Chen Yi-Ching , Chang Gwo-Ching , Hwang Ing-Shiou
Published: March 2023

Visual feedback that reinforces accurate movements may motivate skill acquisition by promoting self-confidence. This study investigated neuromuscular adaptations to visuomotor training with visual feedback with virtual error reduction. Twenty-eight young adults (24.6 ± 1.6 years) were assigned to error reduction (ER) (n = 14) and control (n = 14) groups to train on a bi-rhythmic force task. The ER group received visual feedback and the displayed errors were 50% of the real errors in size. The control group was trained with visual feedback with no reduction in errors. Training-related differences in task accuracy, force behaviors, and motor unit discharge were contrasted between the two groups. The tracking error of the control group progressively declined, whereas the tracking error of the ER group was not evidently reduced in the practice sessions. In the post-test, only the control group exhibited significant task improvements with smaller error size (p = .015) and force enhancement at the target frequencies (p = .001). The motor unit discharge of the control group was training-modulated, as indicated by a reduction of the mean inter-spike interval (p = .018) and smaller low-frequency discharge fluctuations (p = .017) with enhanced firing at the target frequencies of the force task (p = .002). In contrast, the ER group showed no training-related modulation of motor unit behaviors. In conclusion, for young adults, ER feedback does not induce neuromuscular adaptations to the trained visuomotor task, which is conceptually attributable to intrinsic error dead-zones.
Hemotoxic effects of polyethylene microplastics on mice
Authors: Abdel-Zaher Souzan , Mohamed Mahmoud S. , Sayed Alaa El-Din H.
Published: March 2023

Micro- or nanoplastics, which are fragmented or otherwise tiny plastic materials, have long been a source of environmental worry. Microplastics (MPs) have been well documented to alter the physiology and behavior of marine invertebrates. The effects of some of these factors are also seen in larger marine vertebrates, such as fish. More recently, mouse models have been used to investigate the potential impacts of micro- and nanoplastics on host cellular and metabolic damages as well as mammalian gut flora. The impact on erythrocytes, which carry oxygen to all cells, has not yet been determined. Therefore, the current study aims to ascertain the impact of exposure to various MP exposure levels on hematological alterations and biochemical indicators of liver and kidney functions. In this study, a C57BL/6 murine model was concentration-dependently exposed to microplastics (6, 60, and 600 μg/day) for 15 days, followed by 15 days of recovery. The results demonstrated that exposure to 600 μg/day of MPs considerably impacted RBCs’ typical structure, resulting in numerous aberrant shapes. Furthermore, concentration-dependent reductions in hematological markers were observed. Additional biochemical testing revealed that MP exposure impacted the liver and renal functioning. Taken together, the current study reveals the severe impacts of MPs on mouse blood parameters, erythrocyte deformation, and consequently, anemic patterns of the blood.
Simultaneous quantitative analysis and in vitro anti-arthritic effects of five polyphenols from Terminalia chebula
Authors: Liu Fang , Zhan Shipeng , Zhang Pu , Jia Changsheng , Zhu Qingzong , Dai Qing , Yu Mingjie , Cheng Lin , Xiong Lirong , Sun Fengjun , Xia Peiyuan , Zhang Xiao , Hu Jing
Published: March 2023

Background: The fruit of Terminalia chebula has been widely used for a thousand years for treating diarrhea, ulcers, and arthritic diseases in Asian countries. However, the active components of this Traditional Chinese medicine and their mechanisms remain unclear, necessitating further investigation.

Objectives: To perform simultaneous quantitative analysis of five polyphenols in T. chebula and evaluate their anti-arthritic effects including antioxidant and anti-inflammatory activity in vitro.

Materials and methods: Water, 50% water-ethanol, and pure ethanol were used as extract solvents. Quantitative analysis of gallic acid, corilagin, chebulanin, chebulagic acid, and ellagic acid in the three extracts was performed using high-performance liquid chromatography (HPLC). Antioxidant activity was assessed by the 2,2-diphenylpicrylhydrazyl (DPPH) radical-scavenging assay, and anti-inflammatory activity was evaluated by detecting interleukin (IL)-6 and IL-8 expression in IL-1β-stimulated MH7A cells.

Results: The 50% water-ethanol solvent was the optimal solvent yielding the highest total polyphenol content, and the concentrations of chebulanin and chebulagic acid were much higher than those of gallic acid, corilagin, and ellagic acid in the extracts. The DPPH radical-scavenging assay showed that gallic acid and ellagic acid were the strongest antioxidative components, while the other three components showed comparable antioxidative activity. As for the anti-inflammatory effect, chebulanin and chebulagic acid significantly inhibited IL-6 and IL-8 expression at all three concentrations; corilagin and ellagic acid significantly inhibited IL-6 and IL-8 expression at high concentration; and gallic acid could not inhibit IL-8 expression and showed weak inhibition of IL-6 expression in IL-1β-stimulated MH7A cells. Principal component analysis indicated that chebulanin and chebulagic acid were the main components responsible for the anti-arthritic effects of T. chebula.

Conclusion: Our findings highlight the potential anti-arthritic role of chebulanin and chebulagic acid from T. chebula.
Cuttlefish color change as an emerging proxy for ecotoxicology
Authors: Gouveneaux Anaïd , Minet Antoine , Jozet-Alves Christelle , Knigge Thomas , Bustamante Paco , Lacoue-Labarthe Thomas , Bellanger Cécile
Published: March 2023

Lately, behavioral ecotoxicology has flourished because of increasing standardization of analyses of endpoints like movement. However, research tends to focus on a few model species, which limits possibilities of extrapolating and predicting toxicological effects and adverse outcomes at the population and ecosystem level. In this regard, it is recommended to assess critical species-specific behavioral responses in taxa playing key roles in trophic food webs, such as cephalopods. These latter, known as masters of camouflage, display rapid physiological color changes to conceal themselves and adapt to their surrounding environments. The efficiency of this process depends on visual abilities and acuity, information processing, and control of chromatophores dynamics through nervous and hormonal regulation with which many contaminants can interfere. Therefore, the quantitative measurement of color change in cephalopod species could be developed as a powerful endpoint for toxicological risk assessment. Based on a wide body of research having assessed the effect of various environmental stressors (pharmaceutical residues, metals, carbon dioxide, anti-fouling agents) on the camouflage abilities of juvenile common cuttlefish, we discuss the relevance of this species as a toxicological model and address the challenge of color change quantification and standardization through a comparative review of the available measurement techniques.
Identification of a pluripotency-inducing small compound, PLU, that induces callus formation via Heat Shock Protein 90-mediated activation of auxin signaling
Authors: Nakashima Yuki , Kobayashi Yuka , Murao Mizuki , Kato Rika , Endo Hitoshi , Higo Asuka , Iwasaki Rie , Kojima Mikiko , Takebayashi Yumiko , Sato Ayato , Nomoto Mika , Sakakibara Hitoshi , Tada Yasuomi , Itami Kenichiro , Kimura Seisuke , Hagihara Shinya , Torii Keiko U. , Uchida Naoyuki
Published: March 2023

Plants retain the ability to generate a pluripotent tissue called callus by dedifferentiating somatic cells. A pluripotent callus can also be artificially induced by culturing explants with hormone mixtures of auxin and cytokinin, and an entire body can then be regenerated from the callus. Here we identified a pluripotency-inducing small compound, PLU, that induces the formation of callus with tissue regeneration potency without the external application of either auxin or cytokinin. The PLU-induced callus expressed several marker genes related to pluripotency acquisition via lateral root initiation processes. PLU-induced callus formation required activation of the auxin signaling pathway though the amount of active auxin was reduced by PLU treatment. RNA-seq analysis and subsequent experiments revealed that Heat Shock Protein 90 (HSP90) mediates a significant part of the PLU-initiated early events. We also showed that HSP90-dependent induction of TRANSPORT INHIBITOR RESPONSE 1, an auxin receptor gene, is required for the callus formation by PLU. Collectively, this study provides a new tool for manipulating and investigating the induction of plant pluripotency from a different angle from the conventional method with the external application of hormone mixtures.
What can hornworts teach us?
Authors: Frangedakis Eftychios , Marron Alan O. , Waller Manuel , Neubauer Anna , Tse Sze Wai , Yue Yuling , Ruaud Stephanie , Waser Lucas , Sakakibara Keiko , Szövényi Péter
Published: March 2023

The hornworts are a small group of land plants, consisting of only 11 families and approximately 220 species. Despite their small size as a group, their phylogenetic position and unique biology are of great importance. Hornworts, together with mosses and liverworts, form the monophyletic group of bryophytes that is sister to all other land plants (Tracheophytes). It is only recently that hornworts became amenable to experimental investigation with the establishment of Anthoceros agrestis as a model system. In this perspective, we summarize the recent advances in the development of A. agrestis as an experimental system and compare it with other plant model systems. We also discuss how A. agrestis can help to further research in comparative developmental studies across land plants and to solve key questions of plant biology associated with the colonization of the terrestrial environment. Finally, we explore the significance of A. agrestis in crop improvement and synthetic biology applications in general.
Genome-wide analysis of bromodomain gene family in Arabidopsis and rice
Authors: Abiraami T. V. , Sanyal Ravi Prakash , Misra Hari Sharan , Saini Ajay
Published: March 2023

The bromodomain-containing proteins (BRD-proteins) belongs to family of ‘epigenetic mark readers’, integral to epigenetic regulation. The BRD-members contain a conserved ‘bromodomain’ (BRD/BRD-fold: interacts with acetylated-lysine in histones), and several additional domains, making them structurally/functionally diverse. Like animals, plants also contain multiple Brd-homologs, however the extent of their diversity and impact of molecular events (genomic duplications, alternative splicing, AS) therein, is relatively less explored. The present genome-wide analysis of Brd-gene families of Arabidopsis thaliana and Oryza sativa showed extensive diversity in structure of genes/proteins, regulatory elements, expression pattern, domains/motifs, and the bromodomain (w.r.t. length, sequence, location) among the Brd-members. Orthology analysis identified thirteen ortholog groups (OGs), three paralog groups (PGs) and four singleton members (STs). While more than 40% Brd-genes were affected by genomic duplication events in both plants, AS-events affected 60% A. thaliana and 41% O. sativa genes. These molecular events affected various regions (promoters, untranslated regions, exons) of different Brd-members with potential impact on expression and/or structure-function characteristics. RNA-Seq data analysis indicated differences in tissue-specificity and stress response of Brd-members. Analysis by RT-qPCR revealed differential abundance and salt stress response of duplicate A. thaliana and O. sativa Brd-genes. Further analysis of AtBrd gene, AtBrdPG1b showed salinity-induced modulation of splicing pattern. Bromodomain (BRD)-region based phylogenetic analysis placed the A. thaliana and O. sativa homologs into clusters/sub-clusters, mostly consistent with ortholog/paralog groups. The bromodomain-region displayed several conserved signatures in key BRD-fold elements (α-helices, loops), along with variations (1-20 sites) and indels among the BRD-duplicates. Homology modeling and superposition identified structural variations in BRD-folds of divergent and duplicate BRD-members, which might affect their interaction with the chromatin histones, and associated functions. The study also showed contribution of various duplication events in Brd-gene family expansion among diverse plants, including several monocot and dicot plant species.
OsBBX11 on qSTS4 links to salt tolerance at the seeding stage in Oryza sativa L. ssp. Japonica
Authors: Lei Lei , Cao Liangzi , Ding Guohua , Zhou Jinsong , Luo Yu , Bai Liangming , Xia Tianshu , Chen Lei , Wang Jiangxu , Liu Kai , Lei Qingjun , Xie Tingting , Yang Guang , Wang Xueyang , Sun Shichen , Lai Yongcai
Published: March 2023

Rice has been reported to be highly sensitive to salt stress at the seedling stage. However, the lack of target genes that can be used for improving salt tolerance has resulted in several saline soils unsuitable for cultivation and planting. To characterize new salt-tolerant genes, we used 1,002 F2:3 populations derived from Teng-Xi144 and Long-Dao19 crosses as the phenotypic source to systematically characterize seedlings’ survival days and ion concentration under salt stress. Utilizing QTL-seq resequencing technology and a high-density linkage map based on 4,326 SNP markers, we identified qSTS4 as a major QTL influencing seedling salt tolerance, which accounted for 33.14% of the phenotypic variation. Through functional annotation, variation detection and qRT-PCR analysis of genes within 46.9 Kb of qSTS4, it was revealed that there was one SNP in the promoter region of OsBBX11, which resulted in a significant response difference between the two parents to salt stress. Transgenic plants using knockout-based technology and demonstrated that Na+ and K+ in the roots of the functional-loss-type OsBBX11 were translocated largely to the leaves under 120 mmol/L NaCl compared with the wild-type, causing osbbx11 leaves to die after 12 days of salt stress due to an imbalance in osmotic pressure. In conclusion, this study identified OsBBX11 as a salt-tolerance gene, and one SNPs in the OsBBX11 promoter region can be used to identify its interacting transcription factors. This provides a theoretical basis for finding the molecular mechanism of OsBBX11 upstream and downstream regulation of salt tolerance and molecular design breeding in the future.
Transcriptomic, metabolomic, and ATAC-seq analysis reveal the regulatory mechanism of senescence of post-harvest tomato fruit
Authors: Guo Susu , Ji Yanhai , Zheng Yanyan , Watkins Christopher B. , Ma Lili , Wang Qing , Liang Hao , Bai Chunmei , Fu Anzhen , Li Ling , Meng Demei , Liu Mingchi , Zuo Jinhua
Published: March 2023

Several physiological changes occur during fruit storage, which include the regulation of genes, metabolisms and transcription factors. In this study, we compared ‘JF308’ (a normal tomato cultivar) and ‘YS006’ (a storable tomato cultivar) to determine the difference in accumulated metabolites, gene expression, and accessible chromatin regions through metabolome, transcriptome, and ATAC-seq analysis. A total of 1006 metabolites were identified in two cultivars. During storage time, sugars, alcohols and flavonoids were found to be more abundant in ‘YS006’ compared to ‘JF308’ on day 7, 14, and 21, respectively. Differentially expressed genes, which involved in starch and sucrose biosynthesis were observed higher in ‘YS006’. ‘YS006’ had lower expression levels of CesA (cellulose synthase), PL (pectate lyase), EXPA (expansin) and XTH (xyglucan endoglutransglucosylase/hydrolase) than ‘JF308’. The results showed that phenylpropanoid pathway, carbohydrate metabolism and cell wall metabolism play important roles in prolonging the shelf life of tomato (Solanum lycopersicum) fruit. The ATAC-seq analysis revealed that the most significantly up-regulated transcription factors during storage were TCP 2,3,4,5, and 24 in ‘YS006’ compared to ‘JF308’ on day 21. This information on the molecular regulatory mechanisms and metabolic pathways of post-harvest quality changes in tomato fruit provides a theoretical foundation for slowing post-harvest decay and loss, and has theoretical importance and application value in breeding for longer shelf life cultivars.
Phosphate and potash solubilizing bacteria from Moroccan phosphate mine showing antagonism to bacterial canker agent and inducing effective tomato growth promotion
Authors: Bouizgarne B. , Bakki M. , Boutasknit A. , Banane B. , El Ouarrat H. , Ait El Maalem S. , Amenzou A. , Ghousmi A. , Meddich A.
Published: March 2023

Most agricultural soils are facing limited phosphorus availability that challenges modern agriculture. Phosphate solubilizing microbia (PSM) has been explored extensively as potential biofertilizers for plant growth and nutrition, and harnessing phosphate rich areas could provide such beneficial microorganisms. Isolation of PSM from Moroccan rock phosphate led to the selection of two bacterial isolates, Bg22c and Bg32c, showing high solubilization potential. The two isolates were also tested for other in vitro PGPR effects and compared to a non-phosphate solubilizing bacterium Bg15d. In addition to phosphates, Bg22c and Bg32c were able to solubilize insoluble potassium and zinc forms (P, K, and Zn solubilizers) and produce indole-acetic acid (IAA). Mechanisms of solubilization involved production of organic acids as demonstrated by HPLC. In vitro, the isolates Bg22c and Bg15d were able to antagonize the phytopathogenic bacteria Clavibacter michiganensis subsp. michiganensis, causal agent of tomato bacterial canker disease. Phenotypic and molecular identification by 16S rDNA sequencing demonstrated delineation of Bg32c and Bg15d as members of the genus Pseudomonas and Bg22c as member of the genus Serratia. The two isolates Bg22c and Bg32c were further tested either alone or in a consortium and compared to the non-P, K, and Zn solubilizing Pseudomonas strain Bg15d for their efficacy to promote tomato growth and yield. They were also compared to treatment with a conventional NPK fertilizer. Under greenhouse conditions, Pseudomonas strain Bg32c remarkably improved the growth of whole plant height, root length, shoot and root weight, number of leaves and fruits, as well as fruit fresh weight. This strain also induced stomatal conductance enhancement. The strain also improved total soluble phenolic compounds, total sugars, protein, phosphorus and phenolic compounds contents compared to the negative control. All increases were more pronounced in plants inoculated with strain Bg32c in comparison with control and strain Bg15d. The strain Bg32c could be considered a potential candidate for formulation of a biofertilizer in order to improve tomato growth.
Study of cognitive function in patients with severe asymptomatic carotid artery stenosis by a computerized neuropsychological assessment device
Authors: Hu Zhongzhou , Zhang Kun , Qiang Wei , Fan Xiangmin , Chen Zhong
Published: March 2023

Background

Carotid stenosis can lead to stroke and cognitive impairment. Moreover, the cognitive function was assessed mostly by paper and pencil cognitive tests. This study aimed to evaluate the impact of severe asymptomatic carotid artery stenosis (SACAS) on cognitive function by a computerized neuropsychological assessment device (CNAD). The diagnostic value of screening SACAS of the CNAD was analyzed.

Methods

There were 48 patients with ≥70% asymptomatic carotid stenosis and 52 controls without carotid stenosis. Duplex ultrasound defined the degree of stenosis. The differences of cognitive function were analyzed between patients and controls. The relationship of scores of cognitive tests and age were analyzed in the linear regression equation. The diagnostic value of CNAD was evaluated by the receiver operating characteristic (ROC) curve.

Results

Stenosis and control subjects had no statistically significant differences in baseline characteristics. Stenosis patients had worse scores for Stroop color-word test (p = 0.002), one back test (p = 0.013), and identification test (p = 0.006) corresponding to attention and executive ability. The analysis of linear regression equation indicated that cognitive scores of stenosis patients declined faster with age, especially for digit span test, Stroop color-word test, one back test and identification test. In analysis of ROC curve, the Stroop color-word test (p = 0.002), one back test (p = 0.013), and identification test (p = 0.006), and comprehensive index of the three tests (p = 0.001) had the diagnostic value.

Conclusion

The CNAD has evaluation value and screening value for patients with cognitive impairment and SACAS. But it is necessary to update the CNAD and conduct a study with a bigger sample.
Food sharing with friends and acquaintances: A study in preschool boys and girls
Authors: Hallers-Haalboom Elizabeth T. , Vermande Marjolijn M. , van Leeuwen Edwin J. C. , Sterck Elisabeth H. M.
Published: March 2023

Introduction

The current study examined whether preschoolers in a (semi-)natural situation shared more food with friends or acquaintances, and whether this was different between boys and girls, older and younger children, and for preferred and non- preferred food. In order to do so, we replicated and extended the classical work of Birch and Billman in a Dutch sample.

Methods

Participants included 91 children aged between 3 to 6 years (52.7% boys, 93.4% Western European) from a middle- to upper-middle-class neighborhood in the Netherlands.

Results

The results revealed that children shared more non-preferred than preferred food with others. Girls gave more non-preferred food to acquaintances than to friends, whereas boys gave more to friends than to acquaintances. No effect of relationship was found for preferred food. Older children shared more food than younger children. Compared to acquaintances, friends made more active attempts to get food. Moreover, children who were not shared with were just as likely to share food as children who were shared with.

Discussion

Overall, only a small degree of agreement with the original study was found: Some significant findings could not be replicated, and some unconfirmed hypotheses of the original study were supported. The results underscore both the need for replications and studying the effect of social-contextual factors in natural settings.
The infected and the affected: A longitudinal study of the impact of the COVID-19 pandemic on schoolchildren in Florida
Authors: McKune Sarah L. , Acosta Daniel , Fujii Yui , Joyce-Beaulieu Diana , Sayeed Md Abu , Cato Emilee , Flaherty Katelyn E. , Creasy-Marrazzo Ashton , Pu Ruiyu , Kariyawasam Subhashinie , Arukha Anantha , Cummings Derek A. T. , Long Maureen T. , Maurelli Anthony T. , Nelson Eric J.
Published: March 2023

Objectives

To identify risk factors associated with symptoms of anxiety, depression, and obsessive-compulsive disorder (OCD) among children during the 1st year of the COVID-19 pandemic.

Methods

A longitudinal study with three cross-sectional timepoints [April 2020 (n = 273), October 2020 (n = 180), and April 2021 (n = 116)] was conducted at a K-12 public school in Florida. Infection and sero-positivity for SARS-CoV-2 was determined by molecular and serologic approaches. Adjusted odds ratios using mixed effect logistic regression models for symptom-derived indicators of anxiety, depression, and OCD in children in April 2021 are presented; past infection and seropositivity were included in the models.

Results

The prevalence of anxiety, depression, or OCD moved from 47.1, to 57.2, to 42.2% across the three timepoints during the study. By endline of the study, in April 2021, non-white children were at higher risk for depression and OCD. Risk for anxiety, depression, and OCD was associated with students who lost a family member due to COVID-19 and who were identified as at-risk in previous timepoints. Rates of SARS-CoV-2 infection and seropositivity were low and not statistically associated with assessed outcomes.

Conclusions

In situations like the COVID-19 pandemic, targeted mental health interventions and screenings are needed in children and adolescents, especially among minority children.
How does market competition affect supplier-induced demand? An experimental study
Authors: Chen Yefeng , Pan Yiwen , Ding Yuli
Published: March 2023

Introduction

This study investigated the impact of competition on supplier-induced demand in medical markets theoretically and experimentally.

Methods

We employed the framework of credence goods to describe the information asymmetry between physicians and patients, and theoretically derives predictions of physicians' behaviors in monopolistic and competitive markets. Then we conducted behavioral experiments to empirically test the hypotheses.

Results

The theoretical analysis revealed that an honest equilibrium would not exist in a monopolistic market, whereas price competition could induce physicians to reveal their types of treatment cost and provide honest treatments; thus, a competitive equilibrium is superior to that of a monopolistic market. The experimental results only partially supported the theoretical predictions, which showed that the cure rate of patients in a competitive environment was higher than that in a monopolistic market, although supplier-induced demand occurred more frequently. In the experiment, the main channel through which competition improved market efficiency was increased patient consultations through low pricing, as opposed to the theory, which stated that competition would lead to physicians' honest treatment of patients through fair prices.

Discussion

We discovered that the divergence between the theory and the experiment stemmed from the theory's reliance on the assumption that humans are rational and self-interested, which means that they are not as price-sensitive as predicted by theory.
“Community health workers bring value and deserve to be valued too:” Key considerations in improving CHW career advancement opportunities
Authors: Smithwick Julie , Nance Jenesha , Covington-Kolb Sarah , Rodriguez Ashley , Young Mike
Published: March 2023

Introduction

Community health workers (CHWs) are critical members of the public health workforce, who connect the individuals they serve with resources, advocate for communities facing health and racial inequities, and improve the quality of healthcare. However, there are typically limited professional and career building pathways for CHWs, which contribute to low wages and lack of career advancement, further resulting in turnover, attrition, and workforce instability.

Methods

The Center for Community Health Alignment (CCHA), within the Arnold School of Public Health at the University of South Carolina, utilized a mixed-method data collection strategy to provide a more in-depth understanding of this issue and ways that employers, advocates, and CHWs can address it.

Results

Themes across data sources emphasized the importance of retaining skilled and experienced CHWs and educating other health professions about CHWs' critical roles, and reported that doing so will result in decreased attrition professional growth, and improved program quality. CHWs and allies concluded that higher wages, valuing lived experience over formal education, and participation in additional training opportunities should be the primary factors considered for career advancement.

Discussion

Utilizing input from experienced CHWs and CHW allies nationally, this article describes the importance of supporting CHW career advancement, shares best practices, and suggestions for designing strategies that organizations/employers can use to improve CHW career pathways to better support the CHW workforce and reduce attrition.
Symmetrical awareness network for cross-site ultrasound thyroid nodule segmentation
Authors: Ma Wenxuan , Li Xiaopeng , Zou Lian , Fan Cien , Wu Meng
Published: March 2023

Recent years have seen remarkable progress of learning-based methods on Ultrasound Thyroid Nodules segmentation. However, with very limited annotations, the multi-site training data from different domains makes the task remain challenging. Due to domain shift, the existing methods cannot be well generalized to the out-of-set data, which limits the practical application of deep learning in the field of medical imaging. In this work, we propose an effective domain adaptation framework which consists of a bidirectional image translation module and two symmetrical image segmentation modules. The framework improves the generalization ability of deep neural networks in medical image segmentation. The image translation module conducts the mutual conversion between the source domain and the target domain, while the symmetrical image segmentation modules perform image segmentation tasks in both domains. Besides, we utilize adversarial constraint to further bridge the domain gap in feature space. Meanwhile, a consistency loss is also utilized to make the training process more stable and efficient. Experiments on a multi-site ultrasound thyroid nodule dataset achieve 96.22% for PA and 87.06% for DSC in average, demonstrating that our method performs competitively in cross-domain generalization ability with state-of-the-art segmentation methods.
The impact of the use of personal-protective-equipment on the minimization of effects of exposure to pesticides among farm-workers in India
Authors: Lari Summaiya , Yamagani Praveen , Pandiyan Arun , Vanka Janardhan , Naidu Mohan , Senthil Kumar Balakrishnan , Jee Babban , Jonnalagadda Padmaja R.
Published: March 2023

Introduction

Although excessive use of pesticides and unsafe agricultural practices may contribute to numerous intoxications, the role of PPE (personal-protective-equipment) in the minimization of toxicological effects due to pesticide exposure has not been addressed so far. The present study aimed to assess the impact of the use of PPE on the minimization of effects of exposure to pesticides among farm-workers.

Methods

A community-based follow-up study with questionnaire-based survey and field observations was undertaken among farm-workers (n = 180) of Rangareddy district, Telangana, India. Biomarkers of exposure such as cholinesterase activity, inflammatory markers (TNF-α, IL-1β, IL-6, cortisol, and hs-C reactive protein), nutrients (vitamins A, E), liver function (total protein and A/G ratio, AST and ALT levels) were investigated in the laboratory by following the standard protocols.

Results

Farm-workers who had a mean farming exposure of 18 years of and who neither followed safe pesticide handling practices nor used PPE and also showed reluctance to obey good agricultural practices (GAPs). Inhibition of AChE (acetylcholine esterase) with increased inflammation was found among farm-workers as compared to their respective normal values when they have not used PPE. Linear regression statistical analysis revealed a profound effect on inhibition in the AChE activity and various inflammatory markers with the increase in the duration of pesticide exposure. Further, there was no effect of the duration of pesticide exposure on the levels of vitamins A, E, ALT, AST, total protein, and A/G ratio. Further, intervention studies carried out on the use of PPE provided (commercially available and cost-effective) for 90 days showed a significant reduction in the biomarker levels (p < 0.01).

Conclusion

This study demonstrated the importance of the use of PPE during pesticide applications and other agricultural tasks to minimize pesticide-associated adverse health effects.