bluuebunny/crossref_metadata_2025 huggingface dataset:
The Surface Expression of Semidiurnal Internal Tides near a Strong Source at Hawaii. Part I: Observations and Numerical Predictions*
Authors: Chavanne C. , Flament P. , Carter G. , Merrifield M. , Luther D. , Zaron E. , Gurgel K-W.
Published: January 2010

Observations of semidiurnal currents from high-frequency radio Doppler current meters and moored acoustic Doppler current profilers (ADCPs) in the Kauai Channel, Hawaii, are described and compared with two primitive equation numerical models of the tides. The Kauai Channel, separating the islands of Oahu and Kauai, is a site of strong internal tide generation by the barotropic tides flowing over Kaena Ridge, the subsurface extension of Oahu. The nature and impacts of internal tide generation in the Kauai Channel were intensively studied during the 2002–03 near-field component of the Hawaii Ocean Mixing Experiment.

Comparisons of observed coherent (i.e., phase locked to the astronomical forcing) M2 and S2 surface currents with model predictions show good agreement for the phases, indicating propagation of internal tides away from the ridge. Although the predicted M2 and S2 surface currents are similar (except for their magnitudes), as expected for internal waves at periods closer to each other (12.4 and 12 h, respectively) than to the inertial period (33 h), the observed M2 and S2 surface currents differ significantly. The S2 kinetic energy pattern resembles the predicted pattern. In contrast, the observed structure and magnitude of the more important M2 kinetic energy pattern differs significantly from the model predictions. The models predict a band of enhanced M2 surface kinetic energy 30–40 km from the ridge axis, corresponding to the first surface reflection of internal tide beams generated on the ridge flanks. The beams are clearly observed by the moored ADCPs, albeit with weaker amplitudes than predicted. Observations at the surface show an area of enhanced kinetic energy that is 10–20 km farther away from the ridge than predicted, with weaker magnitude. Observed M2 surface currents also exhibit apparent seasonal variability, with magnitudes weaker in spring 2003 than in fall 2002.

Complex-demodulated semidiurnal currents exhibit significant temporal variability in amplitude and phase, not only because of the interference between semidiurnal constituents (e.g., the spring–neap cycle) but also on shorter and irregular time scales. The result is that ∼20% of semidiurnal energy is incoherent with astronomical forcing. Furthermore, the temporal variability is not spatially coherent; the spatial patterns of semidiurnal kinetic energy resemble those predicted by the numerical models during the strongest spring tides but differ from them at other times. As a result, M2 and S2 kinetic energy patterns phase locked to the astronomical forcing differ from each other. Some features of the observed spatial pattern and amplitude modulations can be qualitatively reproduced by a simple analytical model of the effects of homogeneous barotropic background currents on internal tide beams.
Modeling Wind-Driven Circulation and Landfast Ice-Edge Processes during Polynya Events in Northern Baffin Bay
Authors: Dumont Dany , Gratton Yves , Arbetter Todd E.
Published: January 2010

A high-resolution sea ice–ocean numerical model of the North Water polynya has been developed to study the wind-driven circulation during polynya events. An idealized three-layer stratified ocean is used to initialize the model to characterize the baroclinic response to realistic wind and ice conditions. The model general circulation pattern is mainly forced by an along-channel sea level gradient between the Arctic Ocean and Baffin Bay, which determines the magnitude of the southward Baffin Current, and by an across-channel sea level gradient in Baffin Bay, which drives the northward West Greenland Current (WGC). These two currents are found to be anticorrelated to each other in the Smith Sound area. During strong northerly wind events, occurring quite frequently in the winter–spring period in the polynya, nutrient-rich Baffin Bay waters transported by the WGC are forced toward the Greenland shelf, coinciding with upwelling events along the Greenland coast. Whenever an ice bridge is present (i.e., the polynya exists and is substantially open), upwelling also occurs at the landfast ice edge. In such cases, the total upwelling area is increased by an amount that depends on the form of the ice bridge but could easily double during certain years. The baroclinic circulation associated with the upwelling response includes the formation of a cyclonic eddy attached to the ice edge that is generated during strong northerly wind events. Primary production estimations reveal that upwelling during polynya events plays a significant role in the early spring phytoplankton bloom, suggesting that the disappearance of the polynya as a result of climate change may have profound implications for the entire ecosystem.
Two-Layer Tidal Circulation in a Frictional, Rotating Basin
Authors: Winant Clinton D.
Published: January 2010

The three-dimensional tidal circulation in an elongated basin of arbitrary depth is described with a coupled barotropic and baroclinic two-layer model on the f plane. As long as friction is not dominant, near-standing waves are present on the interface as well as on the surface. The surface pattern is principally determined by the product of the tidal barotropic wavenumber by the basin length. The interface deformation is determined by a baroclinic equivalent, usually a much larger number. As a result, the shape of the interface is characterized by horizontally smaller features than the surface. If the product of the tidal baroclinic wavenumber by the basin width is greater than one, both lateral and axial modes can be excited at the interface. If these modes are near resonant, large internal tides can be forced directly by the co-oscillating surface tide at the basin entrance. The amplitude and phase of the baroclinic component are sensitive functions of the density anomaly and the interface depth. As a result, the phase and amplitude of the interface vary by large amounts with comparatively small changes in those parameters. The model behavior is qualitatively consistent with observations in fjords and straits.
An Examination of the Pressure–Wind Relationship for Intense Tropical Cyclones
Authors: Kieu Chanh Q. , Chen Hua , Zhang Da-Lin
Published: January 2010

In this study, the dynamical constraints underlining the pressure–wind relationship (PWR) for intense tropical cyclones (TCs) are examined with the particular focus on the physical connections between the maximum surface wind (VMAX) and the minimum sea level pressure (PMIN). Use of the Rankine vortex demonstrates that the frictional forcing in the planetary boundary layer (PBL) could explain a sizeable portion of the linear contributions of VMAX to pressure drops. This contribution becomes increasingly important for intense TCs with small eye sizes, in which the radial inflows in the PBL could no longer be neglected. Furthermore, the inclusion of the tangential wind tendency can make an additional contribution to the pressure drops when coupled with the surface friction.

An examination of the double-eyewall configuration reveals that the formation of an outer eyewall or well-organized spiral rainbands complicates the PWR. An analysis of a cloud-resolving simulation of Hurricane Wilma (2005) shows that the outer eyewall could result in the continuous deepening of PMIN even with a constant VMAX. The results presented here suggest that (i) the TC size should be coupled with VMAX rather than being treated as an independent predictor as in the current PWRs, (ii) the TC intensity change should be at least coupled linearly with the radius of VMAX, and (iii) the radial wind in the PBL is of equal importance to the linear contribution of VMAX and its impact should be included in the PWR.
Environment Associated with Deep Moist Convection under SALLJ Conditions: A Case Study
Authors: Borque Paloma , Salio Paola , Nicolini Matilde , García Skabar Yanina
Published: January 2010

The present work focuses on the study of the environmental conditions preceding the development of a group of subtropical mesoscale convective systems over central and northern Argentina on 6–7 February 2003 during the South American Low Level Jet Experiment. This period was characterized by an extreme northerly low-level flow along the eastern Andes foothills [South American low-level jet (SALLJ)]. The entire studied episode was dominated by the presence of a very unstable air mass over northern Argentina and a frontal zone near 40°S. The SALLJ generated an important destabilization of the atmosphere due to the strong humidity and differential temperature advection. Orography provided an extra lifting motion to the configuration of the regional wind field, which was efficient in forcing the initiation of convection. Once convection developed, it moved and regenerated in regions where the convective instability was horizontally homogeneous and stronger.
The Wilson Government’s Responses to ‘The Rape of Greek Democracy’
Authors: Maragkou Konstantina
Published: January 2010

This article is a contribution to the analysis of the era of the Greek Colonels, whose military regime ruled Greece between 1967 and 1974. In particular, it seeks to examine one of its less explored aspects, namely the British authorities’ reactions to the news of the coup. It aims to expose underutilized archival evidence proving that the British government, despite its undisputed dislike of the regime’s dictatorial features, did little to avert the consolidation of the regime’s grasp of power during its early, formative stage. In short, it attempts to offer a coherent narrative about the British responses to the unconstitutional establishment of the Greek Colonels’ regime, while accounting for the factors which led them to adopting their stance.
Leisure Clubs and the Decline of the Weimar Republic: A Reassessment
Authors: Nathaus Klaus
Published: January 2010

In contrast to the common assumption that voluntary associations from male voice choirs to gymnastic clubs mediate political culture, this article stresses that leisure clubs were frequented first and foremost as places where sociability could unfold. This form of social exchange, which suspends differences of social status or political attitudes, is relevant for the development of the political system, as it can work as an antidote against a fundamental politicization of all social relations. In Weimar Germany, leisure clubs did not realize this potential. This article argues that this was mainly the consequence of state authorities interfering with the voluntary sector of leisure.
The Faux Policier in Occupied Paris
Authors: Mouré Kenneth
Published: January 2010

Paris experienced a remarkable surge in crimes by men claiming to be police — faux policiers — during the German occupation. This article describes faux policier methods and identifies the particular circumstances in which they thrived. Economic penury provided the context, but the structure of policing power, the weakness of the Vichy state, and the breakdown of distinctions between legal and illegal behaviour all encouraged the increase in faux policier crime.
Incisor Degeneration in Rats Induced by Vascular Endothelial Growth Factor/Fibroblast Growth Factor Receptor Tyrosine Kinase Inhibition
Authors: Fletcher Anthony M. , Bregman Carla L. , Woicke Jochen , Salcedo Theodora W. , Zidell Robert H. , Janke Helen E. , Fang Hengsheng , Janusz William J. , Schulze Gene E. , Mense Mark G.
Published: January 2010

BMS-645737, an inhibitor of vascular endothelial growth factor (VEGF) receptor-2 and fibroblast growth factor (FGF) receptor-1, has anti-angiogenic activity and was evaluated in nonclinical studies as a treatment for cancer. This article characterizes the BMS-645737-induced clinical, gross, and histologic lesions of incisor teeth in Sprague-Dawley (SD) rats. Rats received 0 800 mg/kg BMS-645737 in a single-dose study or consecutive daily doses of 0 20 mg/kg/day in a 1-month study. The reversibility of these effects was assessed in the 1-month study. White discoloration and fracture of incisors were observed clinically and grossly in the 1-month study. In both studies, dose-dependent histopathologic lesions of incisors were degeneration and/or necrosis of odontoblasts and ameloblasts; decreased mineralization of dentin; inflammation and necrosis of the dental pulp; and edema, congestion, and hemorrhage in the pulp and periodontal tissue adjacent to the enamel organ. Partial recovery was observed at lower doses after a two-week dose-free period in the one-month study. Drug-induced incisor lesions were considered to be related to the pharmacologic inhibitory effects on VEGF and FGF signaling, that is, inhibition of growth and maintenance of small-diameter vessels that support the formation of dentin and enamel in growing teeth and/or to perturbances of function of odontoblasts and ameloblasts or their precursors.
United Over Meals Divided at the Lord’s Table: Christianity and the Unity of the Church in Africa
Authors: Asamoah-Gyadu J. Kwabena
Published: January 2010

Christianity in Africa owes its massive growth of the last 50 years to the Independent and Pentecostal/ charismatic churches. The relationships between these churches and the older mission-founded churches are strained. Ethnic and social factors contribute to the divisions. Christian unity in Africa will require conversion to Christ. The strong African tradition of communal life is destroyed by external forces and inter-African conflicts in which members of the same churches have fought one another. Healing is only possible through reconciliation, which calls for conversion from the sin of breaking the community and neglecting the sanctity of human life. The Global Christian Forum offers a new model of working towards Christian unity, which may be particularly meaningful for Africa.
Global Christian Forum: A New initiative for the Second Century of Ecumenism
Authors: Radano John A.
Published: January 2010

This article looks at the Global Christian Forum (GCF) as a new initiative in the historical context of the modern ecumenical movement and from a Catholic point of view. It puts the GCF in three perspectives: as a new stage in ecumenical development, as part of a turning point in ecumenical history and as a new impulse of the Holy Spirit. By bringing in the Evangelicals and Pentecostals, the GCF has widened the range of church families in conversation with one another. The GCF may begin to make a substantial contribution in the situation since Vatican II in which some critical issues between divided Christians have been solved. The beginning convergence of the two movements that have marked the past century — ecumenical and Pentecostal/evangelical — may be the work of the Holy Spirit.
Following Christ with Great Joy: Christians Called to Reconciliation
Authors: Kishkovsky Leonid
Published: January 2010

A brief description of the 20th century ecumenical journey and the Global Christian Forum (GCF) provides the setting for some specific reflections from the US context and the Orthodox perspective. A development similar to the GCF has led to the formation of Christian Churches Together in the USA which is more inclusive of the five Christian families in the USA (Afro-American, Catholic, Evangelical and Pentecostal, Orthodox, Protestant) than the National Council of Churches. The experience of CCT has shown that the GCF meets an urgent need of our time: enabling all Christian churches to encounter one another in Jesus Christ through the sharing of faith stories. The Orthodox faith story is about the presence of the Kingdom among us, the eschatological character of life, the source of which is the Eucharist, and the joy of seeing the Risen Christ in the neighbor.
Laser Scanner-based End-effector Tracking and Joint Variable Extraction for Heavy Machinery
Authors: Kashani Ali H. , Owen William S. , Himmelman Nicholas , Lawrence Peter D. , Hall Robert A.
Published: January 2010

A survey of mining accidents has revealed that over 30% of all truck loading accidents can be addressed by providing dipper positioning feedback to the shovel operator. In this paper, a novel approach is presented for estimating a mining shovel’s dipper pose to obtain its arm geometry in real-time utilizing a two-dimensional laser scanner. The low spatial resolution of laser scanners and the need for accurate initialization challenge the reliability and accuracy of most laser-scanner-based object tracking methods. This work addresses these issues by using the shovel dipper’s kinematics model and position history, in conjunction with the dipper geometrical model, to track the dipper in space. The proposed method uses a bootstrap particle filter with a distance transformation in order to perform a global search in the workspace. The particle filter’s result is then used as the initial pose for an Iterative Closest Point algorithm that increases the accuracy of the pose estimate. The proposed method can be applied to other laser scanner-based object tracking applications in outdoor environments. Experiments performed on a mining shovel demonstrate the reliability, accuracy, and computational efficiency of the proposed approach. Moreover, using a single proximal sensor can simplify mounting, reduce maintenance costs and machine down time, and enhance tracking reliability.
Bovine Ileal Dome Lymphoepithelial Cells: Endocytosis and Transport ofBrucella abortusStrain 19
Authors: Ackermann M. R. , Cheville N. F. , Deyoe B. L.
Published: January 2010

Ligated ileal loops of calves were inoculated with Brucella abortus and examined at 2, 4, 6, 10, and 24 hours post-inoculation. B. abortus was identified by light and electron microscopy using immunoperoxidase and antibody-coated colloidal gold techniques. B. abortus was detected in vesicles, phagolysosomes, and large vacuoles of lymphoepithelial cells. Numbers of intracellular bacteria decreased with time after inoculation. B. abortus was also seen between and below lymphoepithelial cells and free in the dome interstitium and intestinal lymph vessels. Neutrophils and macrophages in both epithelium and lamina propria contained intact or degraded bacteria within phagosomes, phagolysosomes, and multivesicular bodies. These studies showed that (1) transepithelial migration of B. abortus occurred principally by dome lymphoepithelial cell endocytosis and transport, and (2) B. abortus was degraded by macrophages and neutrophils of the gut-associated lymphoid tissue.
Cilia-associated Respiratory (CAR) Bacillus Infection of Obese Mice
Authors: Griffith J. W. , White W. J. , Danneman P. J. , Lang C. M.
Published: January 2010

Cilia-associated respiratory (CAR) bacillus was identified in respiratory tract lesions of obese mice dying of chronic respiratory disease. Neither Mycoplasma pulmonis nor pathogenic bacteria were isolated from cultures of the lesions at necropsy, but there was serologic and histologic evidence of respiratory virus infection. Cranial-ventral areas of lung were firm and demarcated from unaffected lung at gross examination, and representative tissue sank in water. Microscopically, there was suppurative bronchopneumonia with extensive peribronchiole lymphocyte and plasma cell proliferation. The affected bronchiole epithelium was covered with a sheet of slightly basophilic, filamentous, gram negative bacteria. Bronchioles with lesser amounts of lymphocyte accumulations contained lesser amounts of filamentous bacteria. Bronchioles without filamentous bacteria lining the respiratory epithelium lacked peribronchiole lymphocyte accumulations. There was a high correlation between CAR bacillus-positive serology and the identification of diagnostic histologic lesions. CAR bacillus was readily stained using immunohistochemical methods, and the ultrastructural features were similar to that described in rat infections.
Histopathology of Canine Bone Marrow in Malignant Lymphoproliferative Disorders
Authors: Raskin R. E. , Krehbiel J. D.
Published: January 2010

Bone marrow core biopsies from 63 dogs with malignant lymphoproliferative disorders and leukemic involvement were evaluated. Multicentric lymphoma (44), multiple myeloma (8), chronic lymphocytic leukemia (9), and acute lymphoblastic leukemia (2) were found. Four distinct bone marrow histologic patterns were identified: focal (6), mixed (20), interstitial (28), and packed (9). Of those with focal or mixed patterns, 77% (20/26) had paratrabecular distribution. Stromal changes were infrequent, with 6% (4/63) having necrosis, 3% (2/63) fibrosis, and 6% (4/63) osteolysis. For each condition, the interstitial and mixed patterns were the most common presentations, while focal and packed patterns occurred less frequently. Morphologically, cells of metastatic lesions of lymphoma resembled those of primary sites. Colonization of bone marrow by various cytologic types of lymphoma was independent of the histologic patterns.
Myelofibrosis in Cats with Myelodysplastic Syndrome and Acute Myelogenous Leukemia
Authors: Blue J. T.
Published: January 2010

Bone marrow sections from 44 cats with myelodysplastic syndrome (MDS) or acute myelogenous leukemia (AML) were graded for reticulin content using light microscopic methods. Twenty-seven (61%) of the cats had slight to marked reticulin myelofibrosis. The association of myelofibrosis with possible pathogenetic factors, including megakaryocyte count, intramedullary lymphoid follicles, hemosiderin content, and FeLV antigenemia, was examined. No evidence was found that indicated a causal relationship between myelofibrosis and any of these factors.
Double Labeling Immunohistological Study of African Swine Fever Virus-infected Spleen and Lymph Nodes
Authors: Mínguez I. , Rueda A. , Domínguez J. , Sánchez-Vizcaíno J. M.
Published: January 2010

To identify cells in situ in which African swine fever (ASF) virus is present, a double immunohistological labeling technique was used on sections of ASF-infected spleen and lymph nodes. Cells were identified by an indirect immunoalkaline phosphatase technique using monoclonal antibodies (MoAb) reactive against different leukocyte subsets. ASF virus, detected by a direct immunoperoxidase method using swine immunoglobulin G (IgG) anti-ASF virus antigens, was not present in T helper or in T cytotoxic/suppressor lymphocytes, whereas it was detected in tissue macrophages that reacted with different MoAb (74-22-15, C4, A7, and F2). A large number of cells strongly reactive with MoAb 74-12-4 (T helper lymphocytes) were found in the marginal zone in infected spleen. In infected lymph nodes, these intensely stained cells were found in small numbers. Cells reactive with MoAb 76-2-11 (T cytotoxic/suppressor lymphocytes) were less stained in infected spleen and lymph nodes than in non-infected organs.
Comparison of Histology and Clinical Variables to DNA Ploidy in Canine Mammary Tumors
Authors: Hellmén E. , Lindgren A. , Linell F. , Matsson P. , Nilsson A.
Published: January 2010

Flow cytometric DNA analysis was done on 132 canine mammary tumors from 99 dogs to evaluate the relation to histology and to clinical staging. Seventy-one tumors (54%) were histologically malignant; 38 (54%) of these were aneuploid and 33 (46%) were diploid. Fifty-two (39%) tumors were histologically benign, of which 45 (87%) were diploid and seven (13%) aneuploid. There were nine dysplastic mammae (7%); two were aneuploid and the rest diploid. DNA indices varied from 0.72 to 2.35. Of 58 mammary carcinomas, 25 (43%) were diploid and 33 (57%) were aneuploid (of the latter, 16 showed hypodiploidy and 17 hyperdiploidy with a predominance between DNA index 1.10 and 1.50). Three tumors (two carcinomas and one malignant mixed tumor) were multiploid with two aneuploid cell populations. The histological type varied within eight tumors, and in four of these the DNA index also varied. DNA indices varied within three tumors with uniform morphology. No correlation was found between DNA index and age of the dogs, nor between DNA index and tumor size. No significant differences were found between DNA index and histology, tumor growth pattern, or tumor location. Benign tumors were smaller than carcinomas, which were smaller than malignant mesenchymal tumors. Tumors growing adherent to the skin were larger than those not adherent to the skin. The regional lymph nodes were examined in 33 cases. No significant difference between the mean DNA index and presence of lymph node metastasis was found. These results show the possibility of using flow cytometry for DNA analysis in canine mammary tumors. Further investigations are needed to evaluate DNA ploidy as a diagnostic and prognostic tool in the investigation of canine mammary tumors.
Intermediate Filament Staining in the Cytologic and Histologic Diagnosis of Canine Skin and Soft Tissue Tumors
Authors: Andreasen C. B. , Mahaffey E. A. , Duncan J. R.
Published: January 2010

Formalin-fixed histologic and acetone-fixed cytologic preparations from 87 surgically removed subcutaneous and soft tissue canine tumors were examined for immunoreactivity to cytokeratin. desmin. and vimentin. The avidin-biotin complex (ABC) technique demonstrated immunoreactivity in both preparations. but the intensity and specificity of the reactions were dependent on the primary' antibody. Polyclonal antibodies to cytokeratin were more consistent in immunoreactivity than were polyclonal desmin or vimentin antibodies. The monoclonal antibody proved more satisfactory for demonstrating vimentin than the polyclonal antibody. Greater dilutions of primary antibodies may be used on cytologic preparations than on histologic sections. Evaluation of cytologic preparations may be inconclusive due to background staining, scant cellularity, or poor cytoplasmic preservation.
Idiopathic Cutaneous and Renal Glomerular Vasculopathy of Greyhounds
Authors: Carpenter J. L. , Andelman N. C. , Moore F. M. , King N. W.
Published: January 2010

An idiopathic disease involving skin and occasionally kidneys was seen in kenneled and racing greyhounds. Hemorrhages, fibrinoid arteritis, thrombosis, and infarction with deep, slowly healing ulcers characterized the skin lesions. Peracute renal glomerular necrosis, particularly involving afferent arterioles with intravascular coagulation in glomerular capillaries were the distinctive findings in affected kidneys. A complex pathogenesis of this disorder was suspected in which genetic predisposition plays a prominent role.
Hepatitis and Copper Accumulation in Skye Terriers
Authors: Haywood S. , Rutgers H. C. , Christian M. K.
Published: January 2010

Livers of nine related Skye terriers with liver disease were evaluated for histological changes and copper content. Lesions ranged from hepatocellular degeneration and necrosis (zone 3) with intracanalicular cholestasis and mild inflammation, to chronic hepatitis with cholangioplasia and cirrhosis. Excess copper (801–2,257 μg/g) was related to the severity of cholestasis. Skye terrier hepatitis is a distinct disease entity and may be derived from a disorder of intracellular bile metabolism culminating in disturbed bile secretion and the accumulation of copper.
Equine Juvenile Mandibular Ossifying Fibroma
Authors: Morse C. C. , Saik J. E. , Richardson D. W. , Fetter A. W.
Published: January 2010

Benign proliferative fibro-osseous lesions of the rostral mandible in six young horses are classified as equine juvenile mandibular ossifying fibroma. Histologically there is a characteristic abrupt transition from subgingival fibroblastic stroma to a zone of proliferating osteoblasts that form irregular spicules of osteoid. The layer of proliferating osteoblasts blends with a deeper zone of bony trabeculae rimmed by osteoblasts and separated by intertrabecular spaces of moderate cellular density. Incomplete surgical excision resulted in local recurrence, while rostral mandibulectomies resulted in no recurrence. The predilection for the rostral mandible of young horses coupled with the similar clinical, radiographic, and histological characteristics supports the classification of this lesion as a distinct entity.
Chromatophoromas in Two Species of Hawaiian Butterflyfish, Chaetodon multicinctus and C. miliaris
Authors: Okihiro M. S.
Published: January 2010

Chromatophoromas (cutaneous pigment cell tumors) were seen in two species of butterflyfish, Chaetodon multicinctus and Chaetodon miliaris, over an 11-year period (1976–1987) in waters off the islands of Maui, Lanai, and Molokini in the state of Hawaii. The chromatophore tumors found in the brown-barred butterflyfish, C multicinctus, were predominantly iridophoromas (characterized by the presence of birefringent olive-green crystalline pigment), while the tumors in the lemon butterflyfish, C. miliaris, were primarily melanophoromas (characterized by the presence of melanin pigment). Mixed chromatophoromas, composed of iridophores, melanophores, and undifferentiated chromatophores, were found in both species. The prevalence of chromatophoromas in C. multicinctus off the island of Maui varied from a low of 22–25% in 1976 to a high of 50% in 1987. The estimated prevalence of chromatophoromas in C. miliaris was 2.5% off the island of Molokini in 1976, and 5.0% off Lanai in 1987. The cause or causes of chromatophoromas in these two species of butterflyfish has not been determined.
Lesions in Brangus Cattle with Chediak-Higashi Syndrome
Authors: Ayers J. R. , Leipold H. W. , Padgett G. A.
Published: January 2010

Hair, peripheral blood leukocytes, and other tissues from two related Brangus calves with phenotypic characteristics of Chediak-Higashi syndrome were examined by light and electron microscopy. Enlarged, pleomorphic, cytoplasmic granules, morphologically compatible with lysosomes, were seen in several neutrophils, many eosinophils, renal tubular epithelial cells, and Kupffer cells. Hair shafts of the calves showed irregular distribution and clumping of melanin granules. Severe infection and a possible hemorrhagic tendency were recognized. These Brangus calves represent the third breed of cattle affected with this genetic disease.
Otic Pathology of Caprine β-Mannosidosis
Authors: Render J. A. , Lovell K. L. , Jones M. Z.
Published: January 2010

Caprine β-mannosidosis is an autosomal recessive defect of glycoprotein catabolism with a deficiency of tissue and plasma β-mannosidase activity and tissue accumulation of oligosaccharides within lysosomes. This rapidly fatal genetic disorder of Nubian goats is expressed at birth by a variety of clinical signs including deafness. Affected goats had folded pinnas, and the tympanic cavity was decreased due to multiple, polypoid projections of bone covered by middle ear mucosa which obstructed the view of the cochlear promontory. Numerous cells of the cochlear duct including mesothelial and epithelial cells of Reissner's membrane, mesothelial cells lining the scala tympani, cells of the stria vascularis, numerous supportive cells of the organ of Corti, cochlear hair cells, endothelial cells, perithelial cells, fibroblasts, macrophages, and neurons of the spiral ganglion contained numerous nonstaining intracytoplasmic vacuoles which resulted in distention of affected cells and caused thickening of involved structures. Ultrastructurally, the vacuoles were membrane-bound and consistent with lysosomes. Vacuolated cells were desquamated into the scala vestibuli and scala tympani. This is one of few reports describing light and electron microscopic otic alterations of a storage disease. Goats with β-mannosidosis appear to be good models of hearing loss in patients with storage disease.
Renal Disposition of Amylase, Lipase, and Lysozyme in the Dog
Authors: Jacobs R. M.
Published: January 2010

Normal adult dogs were given intravenously lysine hydrochloride to abolish renal tubular reabsorption. The treatment caused tubular proteinuria. Once forced diuresis was established, fractional clearances for amylase, lipase, and lysozyme increased five-, 18-, and 857-fold over the baseline values, respectively. There was relatively little tubular reabsorption of amylase, and urinary amylase activity remained low. A renal arteriovenous difference in amylase activity was not present. Urinary amylase activity could not be reactivated by the addition of serum or treatment with dithiothreitol. Urinary inhibitors of amylase activity were not detected. Immunoreactive urinary amylase did not exceed kinetically measured urinary amylase. Therefore, the presence of irreversibly inactivated amylase did not explain the low fractional clearance of amylase. A small amount of serum macroamylase was present, but macroamylasemia did not account for canine amylase failing to pass the glomerular filter. It appears that the renal loss of amylase in the dog is not an important excretory route.
Scanning Electron Microscopy of Experimental Keratoconjunctivitis Sicca in Dogs: Cornea and Bulbar Conjunctiva
Authors: Kern T. J. , Erb H. N. , Schaedler J. M. , Dougherty E. P.
Published: January 2010

Keratoconjunctivitis sicca was produced experimentally in 16 beagles by bilateral surgical removal of the lacrimal and nictitans glands; four dogs were not treated, and 12 received tear-replacement therapy on post-operative days 7 through 28. Keratoconjunctivitis sicca was verified by reduction in Schirmer tear test values by post-operative day 6, and there was no response on day 28 to tear-replacement therapy. Corneas of both normal and tear-deficient dogs had polygonal squamous epithelial cells of light and dark electron density by scanning electron microscopy. Light cells had more microvilli and microplicae than dark cells. Conjunctivae were similar to corneas, except for numerous goblet cells on the surface. Corneal dark-cell density and goblet cell density were not different between groups. Goblet cells most often occurred singly in normal dogs, while they were in clusters in tear-deficient dogs. A hypothesis that petrolatum/mineral oil ointment should provide more effective artificial tear replacement than hydroxymethylcellulose drops for tear-deficient dogs could not be confirmed by objective analysis of corneal dark-cell density or conjunctival goblet cell density.
Gut-associated Lymphoid Tissue in the Large Intestine of Calves. I. Distribution and Histology
Authors: Liebler E. M. , Pohlenz J. F. , Woode G. N.
Published: January 2010

Gut-associated lymphoid tissue (GALT) in the large intestine was characterized in 12 calves (10 to 84 days old) obtained at necropsy (7, group A) or healthy animals (5, group B). Patches of mucosal lymphoid follicles were in all calves at ileocecal entrances (ICE), 23–42 cm distal to the ICE in the proximal loop of the ascending colon (proximal colon [PC] patch), and in the terminal rectum. PC patches varied from 8 to 30 cm in length. Solitary lymphoid follicles were found in the cecum of three calves, between the ileocecal entrances and the PC patch in four calves, adjacent to the PC patch in all calves, and in the ampulla recti. GALT occupied 7.8% of the large intestinal wall in animals of group A: 0.6% at the ileocecal entrance, 4.8% in the proximal colon, and 2.4% in the rectum. There were two different types of mucosal lymphoid follicles in group B: propria nodules with lymphoid follicles predominantly in the lamina propria, and lymphoglandular complexes with lymphoid follicles in the submucosa. In three 3-, 6-, and 7-day-old, germfree calves, distinct follicle-associated epithelium covered propria nodules and covering folds in depths of the lymphoglandular complexes; it was characterized by numerous intraepithelial cells and lack of goblet cells.
Viral Antigen Distribution in the Central Nervous System of Cattle Persistently Infected with Bovine Viral Diarrhea Virus
Authors: Fernandez A. , Hewicker M. , Trautwein G. , Pohlenz J. , Liess B.
Published: January 2010

Distribution of viral antigens in the central nervous system of 25 cattle with a persistent bovine viral diarrhea virus (BVDV) infection was studied. Using a polyclonal antiserum produced in pigs and the direct immunofluorescence and immunoperoxidase technique, BVDV antigen was located exclusively in neurons. Predilection sites for viral persistence were cerebral cortex and hippocampus; in other areas of brain and spinal cord, viral antigens were in single neurons or small groups of neurons. There was no morphological evidence of cellular alteration due to viral persistence. Perivascular lymphocytic infiltrations were in affected nervous tissue. It is concluded that the central nervous system is an important location for persistence of BVDV.
Hematological, Osmotic, and Scanning Electron Microscopic Study of Erythrocytes of Dogs Given β-acetylphenylhydrazine
Authors: Akuzawa M. , Matumoto M. , Okamoto K. , Nakashima F. , Shinozaki M. , Morizono M.
Published: January 2010

Hematologic examinations, osmotic fragility tests, and scanning electron microscopy of erythrocytes were done on blood of dogs given 5 mg/kg of β-acetylphenylhydrazine for 5 weeks. Reticulocytes, Heinz bodies, and serum total bilirubin values increased in the 1st week. Reticulocyte numbers peaked in the 2nd week, and reticulocytosis persisted through the 5th week. Erythrocyte, packed cell volume, and hemoglobin values decreased markedly and became lowest in the 2nd week. Mean corpuscular volume increased in the 1st week and remained increased for the duration of treatment. Erythrocyte osmotic fragility was increased after 1 week of treatment. Echinocytes were increased with a peak level of 47.6% at week 1 of treatment. Increased numbers of acanthocytes and schizocytes also were detected.
Spontaneous Regression of Bovine Cutaneous Leukosis
Authors: Okada K. , Yamaguchi A. , Ohshima K. , Numakunai S. , Itoh H. , Seimiya Y. , Koyama H.
Published: January 2010

Biopsies of skin from a 2-year-old heifer with spontaneously regressing dermal leukosis were examined. The heifer was not infected with bovine leukemia virus and was negative for tumor-associated antigens of enzootic bovine lymphosarcoma. By hematological standards for bovine leukemia, the heifer was positive at about 60 days post-occurrence of the disease (POD). At 53 days POD, lymphoblastic neoplastic cells in the dermis reacted with anti-T lymphocyte monoclonal antibody by the avidin biotin peroxidase complex method. The cells had intracytoplasmic clustered dense bodies under electron microscopy. From 53 to 83 days POD, figures of the transepithelial elimination (TE) against neoplastic cells and perivascular infiltration of small lymphocytes were in the dermis. Small lymphocytes reacted with anti-T lymphocyte monoclonal antibody. At necropsy there were no neoplastic lesions; there were flat lymph node-like tissues in the subcutis. Many germinal centers were seen in the lymphatic organs. Blood lymphocytes at 46 days POD were stimulated by phytohemagglutinin-P and concanavalin A. Sera, taken until 75 days POD and at necropsy, showed an inhibitory effect on mitogen-induced blastogenesis of normal bovine lymphocytes. These result suggested the existence of a spontaneous regressive mechanism against neoplastic lesions by TE and tumor immunity.
Intestinal Trichomonads (Tritrichomonas mobilensis) in the Natural Host Saimiri sciureus and Saimiri boliviensis
Authors: Scimeca J. M. , Culberson D. E. , Abee C. R. , Gardner W. A.
Published: January 2010

A retrospective study of cecal and colonic tissues from 28 squirrel monkeys ( Saimiri sciureus and Saimiri boliviensis) demonstrated enteric trichomonads within luminal Crypts. Twenty-One of 28 (75%) had trichomonads in the mucosal epithelium either in cup-like depressions or intraepithelial vacuoles. Organisms were also beneath the superficial luminal mucosal epithelium and between the basement membrane and crypt epitheial cells. Immunoperoxidase staining also identified organisms within the lamina propria and submucosa. Additional histologic changes included mucosal ulceration, multifocal cryptitis, and focal epithelial necrosis. Most areas containing trichomonads did not have an associated inflammatory response.
Lymphocytosis of Large Granular Lymphocytes in Three Dogs
Authors: Wellman M. L. , Couto C. G. , Starkey R. J. , Rojko J. L.
Published: January 2010

The clinical course and hematologic changes of three dogs with lymphocytosis of cells morphologically resembling large granular lymphocytes are presented. Hemograms from all dogs showed leukocytosis with marked lymphocytosis. Lymphocytes were characterized by abundant basophilic cytoplasm containing distinct granules which varied in size and number. Electron microscopically the granules were membrane-bound with an electron-dense core. Lymphocytes from one dog were positive for alkaline phosphatase activity, and lymphocytes from another dog were positive for alpha naphthyl butyrate esterase activity. Lymphocytes from one dog were positive for surface receptors for the crystalline fraction portion of gamma immunoglobulins.
A Sequential Histologic and Immunohistochemical Study of Duck Hepatitis B Virus Infection in Pekin Ducks
Authors: Cullen J. M. , Marion P. L. , Newbold J. E.
Published: January 2010

Twenty-nine Pekin ducks were inoculated with duck hepatitis B virus (DHBV), DHBV-free serum, or saline at 1 day of age. Congenitally DHBV-infected ducks were also studied. Ducks were killed periodically during a 92-week study and examined histologically and immunohistochemically to assess liver and extrahepatic inflammation and to detect and characterize DHBV core antigen tissue distribution. DHBV infection produced an asymptomatic but persistent DHBV viremia in all ducks associated with a mild to moderate transient hepatic inflammation apparent at 3 to 6 weeks post-inoculation and waning afterwards. DHBV core antigen was detected in hepatocyte cytoplasm at 1 week post-inoculation, and by 3 weeks post-inoculation scattered pancreatic acinar and islet cells also contained viral antigen. Small numbers of mononuclear cells in the splenic white pulp also contained viral antigen. Viral antigen persisted in all of these tissues throughout the duration of the experiment. No inflammation or tissue injury was detected in any of the extrahepatic tissues during the course of DHBV infection. One DHBV-injected duck developed a hepatocellular carcinoma at 88 weeks of age. Isolated patches of neoplastic hepatocytes contained cytoplasmic DHBV core antigen. The results of this study indicate that DHBV, like mammalian hepadnavirus, is capable of producing a persistent infection of the liver and several extrahepatic tissues and suggest that persistent infection may be associated with the development of hepatocellular carcinoma.
Clinicopathologic Characterization of Canine Juvenile Cellulitis
Authors: Reimann K. A. , Evans M. G. , Chalifoux L. V. , Turner S. , DeBoer D. J. , King N. W. , Letvin N. L.
Published: January 2010

The syndrome of canine juvenile cellulitis was observed and characterized throughout its clinical course when it occurred spontaneously in a litter of dogs. Histologically, pyogranulomatous inflammation was seen in facial skin and mandibular and superficial cervical lymph nodes of affected dogs. The predominant inflammatory cell characterized by light and electron microscopy and by immunohistochemical staining was an epithelioid macrophage. The same pyogranulomatous inflammatory process was seen in a lymph node anatomically distant from the site of apparent disease. Interestingly, a littermate with neither clinically evident dermal lesions nor lymphadenopathy had histologic evidence of a milder, but similar inflammatory process in a mandibular lymph node. The observation of canine juvenile cellulitis in clusters of dogs between 1 and 4 months of age and its apparent systemic nature suggest an infectious etiology. Bacterial, fungal, or viral agents were not isolated from affected lymph nodes. Attempts to transfer the disease by inoculation of neonatal puppies with tissue from affected dogs were also unsuccessful.
Dyspnea in Aging Rats Due to Disseminated Intravascular Coagulation (DIC)
Authors: Carthew P. , Aldred P. , Hill R. J. , Riley J. , Edwards R. E.
Published: January 2010

During an 18-month oncogenicity study using rats, approximately 10% of the animals developed a form of respiratory distress very similar to that seen in the terminal stages of chronic respiratory disease, commonly associated with Mycoplasma pulmonis infection. Investigation of the lungs of the affected rats revealed not only that they did not have the consolidation usually associated with chronic respiratory disease, but they also appeared macroscopically normal. Further investigation of a number of cases revealed systemic intravascular thrombus formation of the type usually referred to as disseminated intravascular coagulation. Using an antiserum to fibrin we have demonstrated the presence of intravascular fibrin deposits in the lungs of the affected rats and have shown them to be the same as experimentally induced intravascular fibrin deposits induced in rat lungs by the administration of thrombin after blocking the fibrinolytic system. This is the first example of such a phenomenon being recorded in aging rats.
A History of the Pathological Division of the Bureau of Animal Industry, United States Department of Agriculture, Between 1891–1921
Authors: Saunders L. Z.
Published: January 2010

As part of a continuing historical study of the evolution of the discipline of veterinary pathology in North America, this paper relates the role played by the Pathological Division of the Bureau of Animal Industry (BAI), formerly a unit of the United States Department of Agriculture. The work of this division of the BAI during its first three decades is examined with respect to its leadership, activities and attainments, and these are compared with similar activities in veterinary colleges and state experiment stations in the United States and in foreign veterinary colleges and research establishments. The Pathological Division devoted a good deal of its efforts to the production of biologic prophylactic products, with resounding success in controlling blackleg and other diseases. Its other activities were in laboratory diagnostic work and in research into animal diseases. The picture that emerges in those spheres is of an organizational unit that despite its name, made little use of the approaches and methods of pathology, but rather availed itself of the tools of microbiology whether or not these were appropriate. In so doing, it lagged considerably behind the comparable institutions both in the United States and abroad.
Gastrointestinal Aspergillosis and Zygomycosis of Cattle
Authors: Jensen H. E. , Olsen S. N. , Aalbæk B.
Published: January 2010

Gastrointestinal mycosis was diagnosed in 73 lesions of 32 cattle without a history of engorgement, and tissues of 29 animals were examined histopathologically. The omasum was the target organ for infection, followed by the rumen and reticulum. Acute necrohemorrhagic lesions dominated, with infiltration of neutrophils and thrombosis. The etiologic diagnosis was accomplished by indirect immunohistochemical staining of fungal elements with a panel of mono- and polyclonal antibodies raised against fungal antigens. Aspergillosis and zygomycosis were diagnosed in one or more organs of 11 (34.4%) and 20 (62.5%) cattle, respectively, and dual infections were found in three lesions of two animals. Candidosis was diagnosed in only one case. Hematogenous spread of fungi predominantly to the liver was seen in nine animals. Lymphogenic spread of aspergilli to mesenteric and omasal lymph nodes occurred in one and two animals, respectively. Factors that seemed to predispose to mycotic infection included presence of other diseases and intense antimicrobial therapy. These factors and post-partum status, which included 23 of 29 (79.3%) cows, predisposed to mycosis in several ways, e.g., reflux of acidic abomasal contents into the forestomachs, stasis of proventricular content, metabolic dysfunctions, and stress.