bluuebunny/crossref_metadata_2025 huggingface dataset:
Pathways for Intergenerational Transmission of Depression: Differentiating between Positive and Negative Maternal Mental Health
Authors: Tan Ai Peng , Huang Pei , Manahan Aisleen , Lee Marissa , Chan Shi Yu , Ngoh Zhen Ming , Kee Michelle , Phua Desiree , Sathyapalan Anu , Chong Yap Seng , Gluckman Peter , Chen Helen , Fortier Marielle , Daniel Lourdes , Zhou Juan , Law Evelyn , Meaney Michael
Published: October 2024

Our understanding of the pathways by which maternal mental health influences the risk for psychopathology in the offspring remains incomplete despite an obvious importance for prevention. This gap, in part, derives from a largely unique focus on maternal symptoms of psychopathology despite evidence for an influence of positive mental health states on a wide range of neurodevelopmental outcomes in the offspring. In this analysis we used a prospective longitudinal cohort study to compare the influence of positive and negative maternal mental health conditions on offspring language and executive function as potential mediators for the influence of maternal mental health on depressive symptoms in the offspring. Our results show that language and executive function were significantly and specifically correlated to positive and negative maternal mental health, respectively. Both functions were significantly correlated with self-reported depressive symptoms at 9 years of age. A serial mediation model showed that language mediated the relation between positive maternal mental health and childhood depressive symptoms. In contrast, executive function mediated the relation between negative maternal mental health and childhood depressive symptoms. Neuroimaging analysis showed that positive and negative maternal mental health were linked to different offspring functional connectivity profiles and highlight the Orbitofrontal-Somatomotor Network B connectivity as a possible neural pathway for the impact of positive maternal mental health on offspring language ability. These results highlight the different operative pathways that link positive and negative maternal mental health to childhood depressive symptoms and provide potential modifiable targets for early intervention.
A nomogram predicts the risk factors for bleeding connected with biliary stent placement therapy
Authors: He Di , Shen Hechen , Yin Weihua , shu guiming
Published: October 2024

Background: Clinically, the ability to predict intra-and postoperative bleeding during stent placement is limited, particularly in patients with high-risk bleeding factors or coagulation disorders. The aim of this study was to create a nomogram to predict bleeding from stent implantation.
Methods: Medical records were collected on March 25, 2024, for 590 cases.
Logistic regression was used to construct the nomogram, and variable screening was performed through LASSO regression. The area under the curve (AUC) was calculated to determine the discriminative capacity of each model. A calibration curve was used to evaluate the prediction accuracy of the model, and the consistency index (CI) was used to determine the performance of the model. Clinical effectiveness was assessed through the use of decision curve analysis (DCA).
Results：LASSO regression identified four clinical features of patients, which were used to generate a nomogram. The AUC was 0.816 (95% CI, 0.743-0.889) in the training model and 0.804 (95% CI, 0.691-0.916) in the test set model. The proposed nomogram would yield a net gain in forecasting stent insertion bleeding if the threshold likelihood of bleeding fell between 0 and 0.8, as demonstrated by a well-calibrated predictive model and DCA.
Conclusions: Four clinical traits of patients who underwent stent placement were combined to create a nomogram predicting risk of bleeding.
Measuring emotional invalidation in Iranian populations: Persian version of perceived invalidation of emotion scale (P_PIES)
Authors: Rezaei Mehdi , Paripishbar Zahra , khazaei Samaneh
Published: October 2024

The examination of the psychometric properties of the PIES in Eastern cultures has remained unexplored. The current study aims to explore the psychometric features of the PIES in Iranian populations. A total of 2250 participants (clinical sample = 177, student samples = 1762, general population = 311) completed the following questionnaires: Perceived Invalidation of Emotion Scale (PIES), Childhood Trauma Questionnaire-Short form (CTQ-SF), Depression, Anxiety and Stress (DASS-2), and the Difficulties in Emotion Regulation Scale-16 (DERS-16). The psychometric qualities of the PIES were examined using construct, convergent, discriminant, incremental, and predictive validities together with internal consistency and test-retest reliability. The results of exploratory and confirmatory factor analysis revealed that the PIES had a unifactorial structure. Support was found for internal consistency as well as for test-retest reliability. In addition, the PIES scores significantly predicted emotional distress over time and had positive correlations with emotional dysregulation, childhood traumas, anxiety, stress, and depression. The PIES was also found to discriminate the clinical from nonclinical populations. Finally, PIES demonstrated adequate incremental validity. Despite the limitations of the current study and the need for additional research, the findings indicate that the PIES with unifactorial structure is a reliable and valid scale among Iranian populations.
Mouse Tissue imaging by random laser of Plasmonic two dimensional array
Authors: Lateef M. , Aldaim W. A. , Haddawi S. F. , Hamidi S. M.
Published: October 2024

Given the major applications of high resolution, and non-destructive bio-imaging, plasmonic waveguide assisted imaging system based on the random lasing is proposed here by helping micro ring arrays covered with the gold/ graphene layer and Rh6G dye. In order to achieve this objective, we employ a laser writing system to create micro ring arrays and subsequently cover them with a plasmonic gold thin film using a sputtering machine. Additionally, the chemical vapor deposition method is employed to generate the graphene layer. To use this medium as random laser active media, we cover it by Rh6G dye and PVP polymer as the top high index layer to get more localization of light. After theoretically and experimentally evaluating the plasmonic substrate, we use second harmonic generation of Nd: YAG laser as the source, and record the random lasing of the sample under 45 degree via spectrometer. Our results show the samples without PVP layer yield a coherent random laser with about 6 nm and 1.4 nm in the full width at half maximum (FWHM), threshold energy 3.17 mJ and 1.42 mJ for concentrations 10 − 5 and 10 − 4, respectively. While finding the laser threshold and FWHM are decreased by the sample with PVP layer reach from 2.62mJ and 5 nm to 1.95 mJ and 1.2 nm, respectively. This corresponded to the simulation part, in which PVP layer enhanced the field amplitude significantly. Finally, we record the images of mouse tissue by the CCD camera. These findings provided a simple and efficient way for the realization of low-threshold random lasers at low cost.
The Influence of Platelet Indicators on ABO Blood Group-Related Susceptibility to Lung Adenocarcinoma: A Multicenter Retrospective Cohort Study
Authors: Zhang Ting , Xiang Mingfei , Yin Hailin , Dan Jiaqiang , Zu Ruiling , Rao Lubei , zhang Peiying , Wang Yuan , Yang Ran , Zhang Yun , Luo Huaichao
Published: October 2024

Objective
The aim of this study was to investigate the role of platelet indicators in the susceptibility of ABO blood groups to lung adenocarcinoma.
Methods
This was a multicenter retrospective cohort analysis. The study included 528 patients diagnosed with primary lung adenocarcinoma and 528 randomly selected benign patients who were admitted to the Chengdu Fifth People’s Hospital from January 2021 to September 2023. Additionally, it included 1452 patients diagnosed with primary lung adenocarcinoma and 1452 benign patients who were admitted to the Sichuan Cancer Hospital from November 2013 to May 2021. Platelet indicators were studied using an automated blood counter. ABO blood groups were determined by the gel column method using agglutination techniques. Statistical analysis was performed using Chi-square tests, independent-samples T-tests, ANOVA, and logistic regression.
Results
Chi-square analysis showed that there was a difference in ABO blood group composition between the lung adenocarcinoma group and the control group(p < 0.001). Independent-samples T-tests showed that MPV was significantly higher in lung adenocarcinoma than in control group(p = 0.025). The ANOVA of ABO blood groups and platelet indicators in lung adenocarcinoma showed that the average platelet count (PLT) of O group blood was higher than that of B group blood(p = 0.037), while the mean platelet volume (MPV) was significantly lower than that of A group, B group and AB group blood(p = 0.009). After adjusting for gender and age, logistic regression analysis showed that the risk of lung adenocarcinoma in blood groups O and B was higher than that in blood group A (p < 0.05 for each) and blood group AB (p < 0.05 for each), regardless of whether platelet indicators were added. Meanwhile, logistic regression showed that high platelet volume (MPV) was a risk factor for lung adenocarcinoma (p = 0.029).
Conclusion
Our findings suggest that platelet indicators are respectively associated with ABO blood groups and lung adenocarcinoma. However, Platelet Indicators may not influence the ABO blood groups related Susceptibility to Lung Adenocarcinoma.
Rabies-Preventive Health Program for Mothers in Maternal and Child Health Centers
Authors: baraka Samia Ibrahim Mabrouk , Nour Rania Abd Elmohsen Abo El , Abdelzaher M. A. , –Nasr Eman Mahmoud Seif El
Published: October 2024

Background: Rabies spreads to people and animals via saliva, usually through bites, scratches, or direct contact with mucosa (e.g. eyes, mouth, or open wounds). Rabies remains a major public health problem in Egypt, with an estimated 60 people dying annually from the disease.
The aim of this study was to assess the effect of preventive program on mothers' awareness for prevention of rabies.
Study Design: Quasi- experimental research design was utilized in this study.
Setting: This study was conducted at three major Maternal and child centers (Botors, Embaby and Segar) in Tanta City.
Sample: A total sample of 150 mothers who attended the child clinic with children aged 6-12 years.
Tools: three tools were developed by researchers were used for data collection. A Structured interviewing questionnaire for assessment of mothers’ knowledge, attitudes and practices regarding rabies Tool I, Mothers' knowledge regarding rabies questnnaire. This tool consisted of two parts: demographic characteristics of the studied mothers and mothers’ knowledge about rabies. Tool II, Rabies attitudinal Likert scale. Tool III, Mothers reported practice Questionnaire.
Results: from the obtained results, it was found that, a statistically significant correlation was found between mothers' total knowledge scores, attitude and total practice scores, both before and after the implementation of the rabies prevention program.
Conclusion: It was concluded that the rabies- preventive health program had positive impact on mother’s knowledge, attitude and practices regarding rabies.
Recommendations: dissemination of the program to the public through mass media to increase their knowledge; improve attitude and practices regarding rabies and its preventive measures.
The relationship of retinal nerve fiber layer thickness and visual field parameter in eight sectors of primary open-angle glaucoma with high myopia based on spectral-domain OCT
Authors: Ruan Jia-Li , Qiu Chen , Qian Shaohong
Published: October 2024

To assess the structure-function relationship of retinal nerve fiber layer (RNFL) thickness in eight sectors based on spectral-domain optical coherence tomography (OCT) with visual field deviation in primary open-angle glaucoma(POAG) patients with high myopia. 225 eyes of 128 patients all diagnosed with POAG were enrolled and stratified by axial length(AL) into moderate ( 25mm ≤ AL < 25.99mm, n = 70 eyes), high (26mm ≤ AL < 26.99mm, n = 76 eyes) and super-high (27mm ≤ AL < 30mm, n = 79 eyes) myopia POAG groups. Data of RNFL thickness and visual field parameters was separated in eight sectors: superotemporal(ST), superonasal(SN), nasal-up(NU), nasal-low(NL), inferonasal(IN), inferotemporal(IT), temporal-low(TL), temporal-up(TU). The Pearson correlation coefficient analysis showed strong structure-function correlation in ST/IT (R = 0.654 to 0.741, p < 0.001) sectors despite AL elongation from 25mm to 30mm in myopia POAG. Also we found decreasing correlation in TL/TU/IN/SN (R = 0.264 to 0.595, p < 0.01) sectors with AL elongation in myopia POAG, with the changed node occurred in 27mm for IN/SN and in 26mm for TL/TU. Specially in NU/NL, the weak correlation(R = 0.233 to 0.381, p < 0.05) became no correlation(p > 0.05) when AL elongated from 25mm to 27mm and above. These descriptions may be helpful to observe and monitor the progression in POAG with high myopia.
Key Cancer Glycosylation-related Genes Analysis: Regulatory, Therapeutic, and Prognostic Insights
Authors: Wang Yida , Chen Xuanming , Jin Xiangyu , Bao Jinku
Published: October 2024

Background: Glycosylation, a key post-translational modification, involves glycosylation-related genes (GRGs) that are crucial in cancer development. Despite their significance, the roles of GRGs in cancer pathways remain insufficiently understood. This study aims to elucidate the function of GRGs across different cancer types, assessing their potential impact on treatment and prognosis.
Methods: We conducted an extensive analysis of over 9,000 samples spanning 33 cancer types, focusing on genomic alterations, cluster analysis, pathway correlations, tumor immunity, miRNA interactions, and identification of therapeutic agents targeting GRGs.
Results: Our genomic landscape analysis identified significant events such as the amplification of B4GALT1 and POMGNT1, co-mutations of POMGNT1-GALNT3-FUT3, and co-amplifications of POMGNT1-B4GALT2. Cluster analysis based on GRG expression revealed four distinct cancer subtypes. Pathway analysis demonstrated a positive correlation between GRGs and both E2F_TARGET and p53_PATHWAY. Additionally, GRGs were involved in tumor immunity and predicted responses to PD-1 blockade therapy. A network of miRNA-GRG interactions was uncovered, and GRG expression was predictive of patient survival across 21 cancer types. We also identified potential therapeutic agents targeting key GRGs.
Conclusions: This comprehensive analysis of GRGs provides critical insights into their roles in cancer development, prognosis, and therapy, offering a robust foundation for future functional and therapeutic studies in oncology.
Evaluation of S. uric acid in COPD patients
Authors: Hendy Rasha Mohamed , Elsaid Salwa Hassan Mohammed
Published: October 2024

Airway inflammation and imbalance between oxidant/anti-oxidant mechanisms are postulated to play a major role in the pathogenesis of COPD. The work aimed to evaluate serum uric acid among COPD patients
Patients and methods: A retrospective cross-sectional study included 50 COPD patients, who attended Benha University Hospital from March 2022 to November 2023. All patients were subjected to the following; written consent from the patient, full history and clinical examination, ABG, CXR (P-A), pulmonary function tests, measuring serum creatinine and urea, measuring serum uric acid by enzymatic colorimetric assay in a fully automated analyzer.
Results: There was a highly significant negative correlation between patients’ serum uric acid levels and FEV1.There was a highly significant negative correlation between patients’ serum uric acid levels and FEV1̸ FVC
Conclusion: uric acid could be used as a chemical biomarker in predicting COPD severity and staging.
Employing Metagenomic Next-Generation Sequencing for Enhanced Surveillance of Respiratory Viruses in Fever Clinics: A Comparative Analysis in the Context of the COVID-19 Pandemic in a General Hospital
Authors: An Shu , Du Longting , Zhong Pengqiang , Wang Ruizhi , Liu qiangqiang , Li Baisheng , Yang Juhua , Liu Huifang , Xiong Yan , Ding Tao , Du Xiangjun , Chen Peisong
Published: October 2024

Background: Clinical surveillance in hospitals, especially in fever clinic, plays a critical role in detecting and managing emerging infectious diseases, serving as an initial alert system for public health epidemics. However, current clinical surveillance networks lack effective methods for comprehensive viral monitoring.
Aim and Methods: This study aimed to establish a pathogen monitoring method using metagenomic next-generation sequencing (mNGS) for pooled specimens in fever clinics, enhancing the capacity for broad-spectrum viral surveillance. We randomly selected oropharyngeal swab specimens from patients at the Fever Clinic who underwent SARS-CoV-2 nucleic acid testing between June 2022 and June 2023. These specimens were pooled, nucleic acids were extracted following standardized protocols, and pathogens were identified through the mNGS technique.
Results: Our results indicated that mNGS for pooled samples exhibited RNA viral detection efficiency equivalent to that of targeted next-generation sequencing (tNGS) for individual samples. Data obtained from mNGS demonstrated a strong correlation with influenza surveillance data from the Guangzhou CDC. Our surveillance method adeptly tracked the progression of the H3N2 epidemic through June 2023 and pinpointed the spread of COVID-19 by late November 2023. Additionally, our analysis exposed notable variations in the respiratory viral spectrum among patients before and after the COVID-19 outbreak.
Conclusion: Utilizing mNGS for mixed-sample detection in fever clinics proved effective and feasible for pathogen surveillance. The approach enhanced understanding of respiratory virus epidemiology, supporting reduced circulation of non-SARS-CoV-2 respiratory viruses during and after the COVID-19 pandemic. This innovative method strengthens public health surveillance by enabling timely detection and response to respiratory virus outbreaks.
STEPWISE management of uncontrolled hypertension in primary care: A cluster randomised controlled trial
Authors: Kilic Birsen , Biermans Marion C.J. , Bots Michiel L. , Wel Mark van der , de Grauw Wim J.C. , Rutten Frans H. , Hollander Monika
Published: October 2024

Background
Uncontrolled hypertension is common and a major health problem because it increases the risk of cardiovascular events. We aim to assess whether a stepwise work-up management strategy for patients with uncontrolled hypertension in primary care would result in better blood pressure control compared to usual care.
Methods
A pragmatic, cluster-randomised controlled trial (cRCT). General practitioners (GPs) from 22 practices (10 intervention arm; 12 usual care) recruited 106 patients aged 18-80 years who had an office blood pressure (BP) >140/90 mmHg and were prescribed three or more antihypertensive drugs from different therapeutic classes for three or more months in an adequate dose. This study was conducted between October 2018 and June 2021.
The intervention was a stepwise approach comprising of (i) 24-hour blood pressure measurements, (ii) dedicated attention on intake of blood pressure raising compounds, (iii) on lifestyle and on compliance to and (iv) optimalisation of medication, and (v) referral to specialist care, when appropriate. The control group received usual care. Primary outcome was the difference in 24-hours systolic BP between intervention and usual care measured at 8 months after baseline.
Results
Neither 24-hour nor office systolic BP 8 months after baseline did significantly differ between intervention and usual care arm: 136.9 mmHg versus 132.6 mmHg (p=0.15) and 146.1 mmHg versus 147.6 mmHg (p = 0.51), respectively. No significant differences across groups were seen in number of prescribed BP lowering medication: 2.98 versus 3.11, or controlled hypertension after 8 months: 22% versus 28%.
Conclusions
Our results are compatible with the notion that a pragmatic but structured stepwise treatment of uncontrolled hypertension does not result in lower 24 hour or office BP values as compared to usual primary care.
Trial registration: NTR7304 (www.trialregister.nl/trial/7099)
LncRNA SERPINB9P1 mitigates cerebral injury induced by oxygen‒glucose deprivation/reoxygenation by interacting with HSPA2
Authors: Lv Miao , Song Xiaoxiao , Wang Weitao , Li Jiale , Chen Jiewen , Huang Xiaolan , Su Li , Gu Lian
Published: October 2024

Dysregulation of long non-coding RNAs (lncRNAs) is implicated in the pathophysiology of ischemic stroke (IS). However, the molecular mechanism of the lncRNA SERPINB9P1 in IS remains unclear. Our study aimed to explore the role and molecular mechanism of the lncRNA SERPINB9P1 in IS. This study revealed downregulation of the lncRNA SERPINB9P1 in the peripheral blood of IS patients, which was corroborated by the GSE140275 dataset. Furthermore, high lncRNA SERPINB9P1 expression was associated with lower National Institutes of Health Stroke Scale (NIHSS) scores and favorable outcome. Clinically, lncRNA SERPINB9P1 expression was correlated with inflammation and coagulation parameters in IS patients. Furthermore, lncRNA SERPINB9P1 silencing inhibited cell viability, induced apoptosis and inflammatory response under oxygen–glucose deprivation/reperfusion ; however, these effects were reversed upon its overexpression.Additionally, Chromatin Isolation by RNA Purification and mass spectrometry (CHIRP-MS) and western blot confirmed that the lncRNA SERPINB9P1was involved in the pathological process of IS through binding to heat shock protein 2 (HSPA2). HSPA2 was upregulated in IS patients, and its protein interaction network was significantly enriched in IS-related pathways. In conclusion, the lncRNA SERPINB9P1 may ameliorate neurological injury in IS patients by interacting with the HSPA2 protein and engaging in IS-related pathways, providing new insights into treatment strategies for IS.
A nomogram for predicting aldosterone-renin ratio in patients with hypertension
Authors: Li Xuehan , Yang Yulu , Liu Changhu , Wu Jiacheng , Huang Jianwu , chen Hao , Wang Yalei , Qiu Zhihua , Zhou Zihua
Published: October 2024

Background: It’s necessary to assess activity of renin-angiotensin-aldosterone system(RAAS) in patients with hypertension by means of orthostatic aldosterone-renin ratio(ARR) which is demanding and not available to those primary hospitals. A novel and portable prediction tool is highly desirable to distinguish abnormal ARR in those patients and guide hypertension therapy to some degree.
Methods: Clinical characteristics and laboratory data of 1,212 patients with hypertension were collected for modeling and randomly divided into a training cohort (865 of 1,212, 70%) and an internal validation cohort (347 of 1,212, 30%). Then, predictors for ARR were extracted to construct a nomogram model based on regression analysis of the training set. Receiver operating characteristics (ROC), calibration plots and decision curve analyses (DCA) were applied to evaluate the model.
Conclusions: Five predictors were adopted to the nomogram including Na-K ratio, gender, serum chloridion(Cl-), estimated glomerular filtration rate(eGFR) and urinary pH. Based on this nomogram, the area under the curve(AUC) was 0.756 (95% CI: 0.71-0.80, p < 0.05) in the training set and 0.725 (95% CI: 0.64-0.81, p < 0.05) in the validation set. The calibration curves exhibited great agreement between the predictive risk of the model and the actual risk and the DCA also showed good clinical benefit.
Conclusion: We have firstly developed a novel nomogram to predict abnormal ARR in hypertensive individuals based on routine biochemical variables.
Finite element analysis of the influence of perioral force on alveolar ridge healing in areas missing maxillary anterior teeth
Authors: Li An-Ke , He Yan-Ting , Li Xuan , Han Cheng-Yuan , Dong Jian-Yong , Zhang Kai-Qi , Cui Jun
Published: October 2024

Objective
To study the biomechanical changes induced by differences in perioral force in patients with missing anterior maxillary teeth at rest via finite element analysis (FEA).
Methods
Using conical beam CT (CBCT) images of a healthy person, models of the complete maxillary anterior dental region (Model A) and maxillary anterior dental region with a missing left maxillary central incisor (Model B) were constructed. The labial and palatine alveolar bone and tooth surface of the bilateral incisor and cusp regions were selected as the application sites, the resting perioral force was applied perpendicular to the tissue surface, and the changes in maxillary stress and displacement after the perioral force was simulated were analyzed.
Results
Compared with those of Model A, the labial alveolar bone in the missing tooth area of Model B exhibited obvious stress concentration and displacement under the action of perioral force.
Conclusion
At rest, perioral force, especially the soft tissue pressure of the lip, has an adverse effect on alveolar ridge healing in areas with missing maxillary anterior teeth.
Unraveling the influence of Indoxyl Sulfate and apixaban in drug metabolism and elimination: Is sex a major player?
Authors: burtey stephane , Pina-Beltran Blanca , Poitevin Stephane , Dimitrov Daniel , Rodriguez Julio Saez , giot matthieu , Peinado Jorge , Zdrahal Zbynek , Potesil David , Putska Vaclav , McKay Nathalie
Published: October 2024

Chronic Kidney Disease (CKD) is associated with heightened risk of thrombosis. Prescription of anticoagulants is key to manage it; however, CKD patients have shown an increased risk of bleeding under anticoagulation therapy compared to non-CKD patients. We hypothesized that the accumulation of uremic toxins, particularly of indoxyl sulfate (IS) could modify drug metabolism. Our intoxication model shows that higher doses of IS and apixaban accumulate in the plasma of female mice as a result of expression differences in efflux transporters and cytochromes in the liver, ileum and kidneys, when compared to males. Furthermore, we found that accumulation of apixaban in females contributes to increased bleeding. q-RT PCR analysis of liver samples revealed elevated Sult1a1 but reduced Abcg2 and Cyp3a11 in female mice, while in the kidneys the expression rates of Oat1 and Oat3 were respectively lower and higher than those observed in males, potentially affecting drug clearance. Whole proteomics liver analysis confirmed the previous mRNA results at the protein level and revealed that sex had a major influence in regulating both coagulation and drug metabolism pathways. Thus, our findings underline the need for inclusive clinical and preclinical trials to accurately reflect sex-specific metabolic variations, and to consider CKD-specific changes to optimize dosing, minimize side effects, and improve patient outcomes.
Ethnic Group Differences in Economic Stress and Out-Group Prosocial Behaviors During the Covid-19 Pandemic: A Cultural Process Model
Authors: Carlo Gustavo , Davis Alexandra N , Torres Marixza , Sanders Heidi , Cruz Lynda J.
Published: October 2024

The goal of the current study was to examine intervening culture and family related mechanisms in the links between pandemic-related economic stress and young adults’ ethnic out-group prosocial behaviors. Participants were 467 young adults (mean age = 22.58, range = 19-27 years old) from across the U.S. (50.1% female; 55.7% identified White as their racial group, 20.3% Black/African American, 12.2% Asian American, 8.0% identified as other, including Latino/a participants who did not identify as White). Participants completed online measures of their experiences with economic stress during the COVID-19 pandemic, their ethnic identity, identification with filial piety as a culture value, and their own tendencies to engage in six forms of out-group prosocial behaviors. The results demonstrated indirect links between economic stress and multiple forms of out-group prosocial behaviors via ethnic identity and filial piety as well as direct links between economic stress and ethnic identity and out-group prosocial behaviors. Interestingly, there were ethnic group differences that emerged in the direct paths such that economic stress was a particularly strong predictor of prosocial behaviors for ethnic majority participants, and ethnic identity was stronger for ethnic minority participants. Discussion will explore ethnic differences in these associations as well as the role of cultural and family values as important mechanisms in the links between pandemic-related economic stress and out-group prosocial behaviors.
WITHDRAWN: Comparison of Upper Arm Ports and Peripherally Inserted Central Catheters in Patients with Nasopharyngeal Carcinoma: A Randomized Controlled Trial.
Authors: Xiao Maofang , Li Jia , Xiao Caiqiong , Hu Wen , Fan Yuying , Qin Huiying
Published: October 2024

Objective
To compare upper arm ports (UAPs) and peripherally inserted central catheters (PICCs) in patients with nasopharyngeal cancer in terms of complications and health economics.
Methods
A randomized controlled trial was conducted with a total of 126 patients. The patients were randomly assigned to the experimental group (UAP) or the control group (PICC). The outcomes were observed from the day of placement until extubation. Demographic data, data related to catheter placement, and the placement effects were collected and analyzed using SPSS 25.0.
Results
Catheters were successfully inserted in 123 out of the 126 patients (61 in the experimental group and 62 in the control group). Compared with the control group, the experimental group had a lower incidence of complications after placement (21.3% vs. 54.8%, P < 0.001). This included lower incidences of catheter dislodgement (0% vs. 21.0%, P < 0.001), catheter occlusion (1.6% vs. 12.9%, P = 0.032), wound oozing (4.9% vs. 33.9%, P < 0.001), and medical adhesive-related skin injury (4.9% vs. 14.9%, P = 0.025). Additionally, the severity of both wound oozing (P < 0.001) and medical adhesive-related skin injury (P = 0.014) was lower in the experimental group. Although the surgical costs were higher in the experimental group, when considering overall healthcare costs and health outcomes, the net benefits became equal between the two groups after 2.8 months. Beyond this point, the net benefits of the experimental group surpassed those of the control group, with the difference increasing as the dwell time of the catheter increased.
Conclusion
Compared with PICCs, UAPs resulted in lower incidences of complications post-placement including wound oozing, medical adhesive-related skin injury, catheter dislodgement, and catheter occlusion. Meanwhile, UAPs showed superior cost-effectiveness. Thus, UAPs are recommended for clinical application as a preferred choice over PICCs.
Trial registration.
This study was registered at clinicaltrials.gov. (ChiCTR2100044272, registered on 14 March 2021).
LncRNA GUSBP11/miR-28-5p/PAQR7 axis regulates the phosphorylation of E-cadherin and N-cadherin on the migration of papillary thyroid carcinoma cells
Authors: Shi Ji , Xiang Yuan , Liu Yi-Meng , Guo Hong-Shan , Li Han-Ning , Liao Xing-Hua
Published: October 2024

Papillary thyroid carcinoma represents the most prevalent form of thyroid cancer, exhibiting the lowest degree of malignancy. It constitutes approximately 85% of all thyroid cancer cases. This cancer type can manifest at any age but is predominantly observed in children or young women under the age of 40. The aberrant expression of long non-coding RNA (lncRNA) and messenger RNA (mRNA) has been identified as a crucial factor in the pathogenesis of this disease. Our analysis of existing databases revealed that lncRNA GUSBP11 and the membrane progesterone receptor PAQR7 are significantly overexpressed in thyroid cancer, demonstrating a notable positive correlation between them. However, RNA sequencing analysis indicated the absence of a direct relationship between these molecules. Through a comprehensive pooled analysis across multiple studies, we discovered a direct link between miR-28-5p and both lncRNA GUSBP11 and mRNA PAQR7, which were found to be significantly downregulated in thyroid cancer cases. This research endeavors to lay a novel foundation for the development of targeted therapy for thyroid cancer, focusing on the interaction between lncRNA, miRNA, and mRNA.
Finger Dissection Open Radical Prostatectomy Offers Equal Early Functional Results with Robotic Prostatectomy in the Robotic Era: A Comparative Study
Authors: Atik Yavuz Tarik , Gul Deniz , Cimen Haci Ibrahim , Erdik Anil , Uysal Burak , Kose Osman , Saglam Hasan Salih
Published: October 2024

BACKGROUND: Apical dissection is a crucial step of radical prostatectomy (RP) for postoperative functional outcomes. Robot-assisted laparoscopic RP (RALRP) represents a good visualization for this stage and bleeding during ligation of dorsal vein complex (DVC) is a challenging problem in open RP (ORP). In our study, the results of finger dissection technique in open RP were evaluated and compared with RALRP.
METHODS: We retrospectively reviewed 184 patients who underwent RP for clinically localized prostate cancer. Blunt finger dissection technique performed for ligation of DVC in ORP and patients were divided into two groups according to the surgical method (Group 1:RALRP; Group 2:FD-ORP – Finger dissection-ORP). The primary outcomes were continence status and erectile functions. Demographics, operative outcomes, preoperative and postoperative oncological data were also recorded.
RESULTS: Ninety-two patients in each group were enrolled in the study. Demographics and preoperative data were similar. Also, there is no difference between groups in terms of operation time, blood loss, transfusion rates, hospitalization time, and peri- and postoperative complications (p>0.05). Urethral catheterization time was higher in FD-ORP (median day 14 vs. 21, p<0.001). Continence rates (82.6% vs. 89.1%, p=0.439) and erectile functions (mean IIEF: 10.62 vs. 11.34, p=0.107) showed no significant difference between FD-ORP and RALRP in the first year after surgery.
CONCLUSION: Finger dissection of the DVC during ORP is a simple and feasible technique that provides the correct plane on the urethra, resulting in better apical dissection with effective hemostasis. Although it shows similar early functional results as RALRP, more research is needed.
Diagnostic value of perineal neck length, interleukin-6, and fetal fibronectin in preterm birth
Authors: Lu Jianfeng , Lu Juefei , Zhang Xiaoli , Mu Ling , Luo Weiming , Zhang Xiaolei
Published: October 2024

OBJECTIVE:This study aimed to explore the diagnostic value of perineal neck length, vaginal secretion of interleukin-6 (IL-6) and fetal fibronectin (fFN) in preterm birth and their relationship with maternal vaginal flora.
METHODS:A total of 180 patients with threatened preterm birth admitted to our hospital from January 2021 to February 2023 were selected. All patients underwent perineal neck length measurement and vaginal secretion IL-6 and fFN testing. The occurrence of preterm birth was observed, and the clinical data, perineal cervical length, and vaginal secretion IL-6 and fFN levels of patients with and without preterm birth were analyzed.
RESULTS:The length of the perineal cervix in patients with premature birth was (23.30±9.11) mm, which was significantly shorter than that in patients with premature birth (P < 0.05). In addition, the levels of IL-6 and fFN in vaginal secretions of patients with premature birth were (145.54±44.17) pg/ml and (80.44±26.43) μg/L respectively, which were significantly higher than those of patients with non-premature birth (p＜0.05).
CONCLUSION: Perineal neck length, vaginal secretion IL-6 level and fFN level have good predictive value for preterm birth. Furthermore, there was a correlation between these factors and the vaginal microbiota index.
Reproducibility and stability of deep inspiration breath hold and free breath in breast radiotherapy based on real-time 3-dimensional optical surface imaging system
Authors: Wu Junxiang , Yang Feng , Li Jie , Wang Xianliang , Yuan Ke , Xu Lipeng , Wu Fan , Tang Bin , Orlandini Lucia Clara
Published: October 2024

Background
The aim of this study was to evaluate the inter-fraction reproducibility and intra-fraction stability of breast radiotherapy using deep-inspiration breath hold (DIBH) and free breathing (FB) based on an optical surface imaging system (OSIS).
Methods
Seventeen patients (510 breath-hold sessions) treated using a field-in-field (FiF) technique and twenty patients (600 breath-free sessions) treated with a volume-modulated arc therapy (VMAT) technique were included in this retrospective study. All the patients were positioned with the guidance of CBCT and OSIS, and also monitored with OSIS throughout the whole treatment session. Eight setup variations in three directions were extracted from the treatment reports of OSIS for all sessions and were subsequently manually introduced to treatment plans, resulting in a total of 296 perturbed plans. All perturbed plans were recalculated, and the dose volume histograms (DVH) for the target and organs at risk (OAR) were analyzed.
Results
The OSIS and CBCT for both DIBH and FB treatments showed a good agreement of less than 0.12 cm in each direction. The intra-fraction setup errors during DIBH were − 0.06 ± 0.07 cm, 0.12 ± 0.15 cm, and 0.12 ± 0.12 cm in the lateral, longitudinal, and vertical directions, respectively; for FB, the errors were − 0.02 ± 0.12 cm, 0.08 ± 0.18 cm, and 0.14 ± 0.20 cm, respectively. For the target, DIBH plans were more sensitive to positioning errors; the mean deviations in D95 for CTV were 39.78 Gy-40.17 Gy for DIBH and 38.46 Gy-40.52 Gy for FB, respectively. For the OARs, the mean deviations—V10, V20, and Dmean to the heart; V5, V20, and Dmean to the ipsilateral lung; and Dmean to the breast—were lower for the FB plan compared with the DIBH plan.
Conclusion
SGRT can be an important tool for inter-fraction patient positioning and intra-fraction patient respiratory motion management in DIBH and FB breast cancer radiotherapy. The FB technology has greater possibility for the undercoverage of the target volume, while DIBH technology is more likely to result in increases in dose to organs at risk, especially the lung, heart, and breast. Furthermore, the tolerance of optical surface imaging systems could be reduced, indicating a potential method to reduce the dose delivery uncertainty caused by the patient’s respiratory motion.
Inactivation of the conserved protease LonA increases production of xylanase and amylase in Bacillus subtilis
Authors: Wang Biwen , Kes Mariah B.M.J. , Saparoea Anna C.H. van den Berg van , Dugar Gaurav , Luirink Joen , Hamoen Leendert W.
Published: October 2024

Background
Bacillus subtilis is widely used for industrial enzyme production due to its capacity to efficiently secrete proteins. However, secretion efficiency of enzymes varies widely, and optimizing secretion is crucial to make production commercially viable. Previously, we have shown that overexpression of the xylanase XynA lowers expression of Clp protein chaperones, and that inactivation of CtsR, which regulates and represses clp transcription, increases the production of XynA. In the current study, we examined whether the same is the case for overexpression of the α-amylase AmyM from Geobacillus stearothermophilus by B. subtilis, and why XynA shows a different timing of secretion compared to AmyM.
Results
Transcriptome analyses revealed that B. subtilis cells overexpressing AmyM exhibited a distinct profile compared to XynA overexpressing cells, however there were also similarities and in both cases expression of CtsR controlled genes was downregulated. In contrast to XynA, inactivation of CtsR did not improve AmyM production. Upregulation of other protein chaperones, including GroEL/ES and DnaJ/K, by inactivating their transcriptional repressor HrcA, had almost no effect on XynA yields and in fact considerably lowered that of AmyM.
Despite using the same promoter, the production of XynA peaks well before AmyM reaches its optimal secretion rate. Transcriptome and ribosome profiling indicated that this is neither related to transcription nor to translation regulation. We show that the reduced secretion in the stationary phase is partially due to the activity of secreted proteases, but also due to the activity of the intracellular protease LonA. The absence of this protein resulted in a 140 % and 20 % increased production for XynA and AmyM, respectively.
Conclusions
The combination of transcriptome and ribosome profiling offered important information to determine at which cellular level production bottlenecks occurred. This helped us to identify LonA protease as an important factor influencing enzyme production yields in B. subtilis.
Improving care experiences for premenstrual symptoms and disorders in the United Kingdom (UK): A mixed-methods approach
Authors: Funnell E. L. , Martin-Key N. A. , Bahn S.
Published: October 2024

Background
Poor care experiences are reported for premenstrual disorders, which may result in negative outcomes such as distress, reduced healthcare engagement, and delays to diagnosis. Insights from those who have sought care for premenstrual symptoms previously are a valuable resource in identifying characteristics of poor care experiences as well as making recommendations for improvements based on their own experiences.
Method
An online survey was delivered, with participants recruited via social media. Data were explored using descriptive statistics and thematic analysis.
Results
The sample included 339 participants who completed at least 97% of the survey, endorsed premenstrual symptoms in consecutive menstrual cycles, and had sought formal help specifically for premenstrual symptoms. The mean age was 34.66 (SD = 5.88), with the majority identifying as women (n = 332, 97.94%) and white/Caucasian (n = 311, 91.74%). 75.22% (n = 255) felt that care was poor. 44.25% (n = 150) felt their symptoms were not taken seriously. 37.76% perceived a lack of HCP knowledge (n = 128). The majority did not receive recommendations of additional non-formal sources of help (i.e., websites, support groups; 84.96%, n = 288) or lifestyle changes (74.63%, n = 253). Better care experiences were associated with healthcare professionals taking symptoms seriously (U = 1383.00, p < .001), higher perceived healthcare professional knowledge (U = 1370.50, p < .001), and receiving recommendations of additional non-formal sources of help (X2 = 48.251, df = 1, p < .001, Φc = .382) or lifestyle changes (X2 = 7.849, df = 1, p = .005, Φc = .152). Thematic analysis revealed 8 aspects of care improvement: Empathetic care provision; HCP education, understanding, & research; Comprehensive symptom assessment and investigations; Diagnosis; Professional support and treatment provision; Signposting or referral to additional resources or sources of help; Wider healthcare system improvements; and, Patient role/voice and preferences.
Conclusions
Poor care experiences for premenstrual symptoms in the United Kingdom (UK) are characterized by dismissive attitudes and perceived lack of knowledge. Improved training provision for healthcare professionals is required. However, given the healthcare system constraints in the UK, research is needed to explore the feasibility and utility of informational toolkits for healthcare professionals. Utilization of standardized screening tools and patient-centered communication will likely ensure comprehensive assessments and reduce self-advocacy burdens. It is crucial to improve knowledge of therapeutic interventions to reduce barriers to treatment-seeking and enable shared decision-making.
Approach of Dynamic Simulation and Optimization for Coevolutionary Intelligent Manufacturing Cell in Digital Twin
Authors: Leng Sheng , Chen Fuxing , Wei Yunpeng , Yang Xianggui , Wang Cong
Published: October 2024

In real manufacturing industry, the timely identification of weaknesses and precise determination of optimization areas are crucial for efficient workshop production. Traditional plant simulation methods have been widely employed to support decision-making, but the accuracy of these decisions heavily depends on the reliability of the simulation results. Static simulations, which rely on manually set parameters often fall short of meeting these demands. To address this, dynamic simulations incorporating real-time data are essential for generating accurate and actionable insights. A worth-trying approach is proposed therefore in this paper by establishing a plant simulation environment in Digital Twin(DT) utilizing a high-fidelity real-time coevolution model for intelligent manufacturing cells. To get the coevolutionary models in DT system, data modeling and integration, virtual-real event driven method are illustrated. This approach provides a true-to-life workshop simulation environment. By employing variable and customizable optimization strategies, diverse simulation outcomes can be generated, aiding in the selection of the most effective optimization strategies. The development of a coevolutionary DT for a manufacturing cell within an enterprise is presented as a case study, demonstrating the effectiveness of dynamic and combo-strategy production simulations.
Comparing Allogeneic vs. Autologous Hematopoietic Stem Cell Transplantation for Acute Myeloid Leukemia - Evaluating Outcomes Across Age Groups: A Systematic Review
Authors: Bandi Venkata Varshitha , Tarar Pakeeza , Bishara Rafik Maged Naguib , Koneru Hema Manvi , Sarwar Hooria , Sinha Mohit ,   7.	Iana Malasevskaia
Published: October 2024

Background
Acute myeloid leukaemia (AML) impacts various age groups, with hematopoietic stem cell transplantation (HSCT) being an important treatment option, this review compares the outcomes of allogeneic and autologous HSCT, focusing on the influencing factors across different age demographics.
Methods
A comprehensive literature search was conducted across five databases, including PubMed/Medline, Cochrane Library, ScienceDirect, Europe PMC, EBSCO, and ClinicalTrials.gov. The inclusion criteria encompassed randomized controlled trials (RCTs), non-randomized controlled trials, cohort studies, and case-control studies published since inception. After screening, 19 studies were selected for review, all demonstrating moderate to high quality.
Results
A total of 21,977 participants were included across the 19 studies, the majority of which were observational. Key outcomes assessed post-transplantation included overall survival (OS), disease-free survival (DFS), relapse rates, transplant-related mortality (TRM), and relapse-free survival (RFS). Notably, patients with abnormal 17p chromosomes exhibited varying OS based on age, with those under 50 showing better outcomes. An RCT indicated that autologous HSCT resulted in lower TRM and improved OS for children and adolescents, albeit with higher relapse rates compared to allogeneic HSCT. In elderly populations, autologous HSCT showed no significant difference in OS but demonstrated lower non-relapse mortality (NRM) compared to allogeneic HSCT.
Conclusion
Various factors influence HSCT outcomes in AML patients. Autologous HSCT may yield better results for children and adolescents, while allogeneic HSCT appears more effective for adults and the elderly concerning survival and relapse rates, despite some studies indicating a mortality advantage for autologous HSCT.