bluuebunny/crossref_metadata_2025 huggingface dataset:
Urban Informal Food Traders: A Rapid Qualitative Study of COVID-19 Lockdown Measures in South Africa
Authors: Rwafa-Ponela Teurai , Goldstein Susan , Kruger Petronell , Erzse Agnes , Abdool Karim Safura , Hofman Karen
Published: February 2022

Globally, the adoption of COVID-19 containment measures, such as lockdowns, have been used to curb the rapid spread of the pandemic. However, these action regulations have caused substantial challenges to livelihoods. We explored the perceptions and experiences of COVID-19 implications for urban informal food traders in South Africa during the initial lockdown period that lasted five weeks. A rapid qualitative study was conducted during October–November 2020. Twelve key informants (seven men and five women) categorized into informal traders and food system expert groups were interviewed. Data were analyzed thematically using MAXQDA software. Participants perceived informal trading as a main source of livelihood for many individuals. Negative lockdown impacts described included forced business closure, increased food costs and reduced demand. The consensus among participants was that the government’s lack of formal recognition for informal food traders pre-COVID-19 contributed to challenges they faced during the pandemic, as evidenced by their exclusion as “essential service providers’’ at the start of lockdown. Policies that fail to recognize and consider informal food traders during ‘normal’ times lead to widened social inequality gaps among already vulnerable groups during natural disasters and disease outbreaks. In the case of COVID-19 in South Africa, this caused severe hunger, food insecurity and income loss.
Characterization of Biochar Derived from Crop Residues for Soil Amendment, Carbon Sequestration and Energy Use
Authors: Venkatesh Govindarajan , Gopinath Kodigal A. , Reddy Kotha Sammi , Reddy Baddigam Sanjeeva , Prabhakar Mathyam , Srinivasarao Cherukumalli , Visha Kumari Venugopalan , Singh Vinod Kumar
Published: February 2022

The crop residues generated in agricultural fields are mostly considered a burden due to their disposal issues. This study attempts to effectively use pigeon pea stalk (PPS) for biochar production, a promising source as a soil amendment for carbon sequestration and alternative fuel source. PPS was pyrolyzed at different loads and reaction times to optimize the kiln temperature (350–400 °C and 450–500 °C) and changes in physicochemical properties, higher heating value (HHV) and yield were assessed. The results indicated that biochar yield, volatile matter, bulk density, O/C and H/C atomic ratios decreased, whereas fixed carbon, ash content and total porosity increased with increasing kiln temperature across all loads. Biochar produced at 450–500 °C (18 kg load kiln−1) had higher total carbon, nitrogen, phosphorous, recovered total carbon and total nitrogen, total potential carbon and CO2 reduction potential. Biochar produced at 350–400 °C had the maximum cation exchange capability (43.0 cmol kg−1). Biochar has estimated O/C and H/C atomic ratios of 0.07–0.15 and 0.35–0.50, respectively. Biochar exhibited good agronomic characteristics and fulfilled key quality criteria of H/C < 0.7 and O/C < 0.4 for soil carbon sequestration, as described by the European Biochar Certificate and the International Biochar Initiative. The estimated mean residence time and the mass fraction of carbon that would remain after 100 years were consistently greater than 1000 years and 80%, respectively. The biochar produced at 450–500 °C (at 18.0 kg kiln−1) from PPS had higher fixed carbon (65.3%), energy density (1.51), energetic retention efficiency (53%), fuel ratio (4.88), and HHV (25.01 MJ kg−1), as well as lower H/C and O/C ratios, implying that it is suitable for use as an alternative solid fuel.
Suitability Evaluation of Popular Science Tourism Sites in University Towns: Case Study of Guangzhou University Town
Authors: Guo Wei , Wu Da-Fang , Li Yue , Wang Feng-Xi , Ye Yong-Qi , Lin Hua-Wei , Zhang Chi-Fang
Published: February 2022

With the advent of the knowledge economy, universities have increasingly important roles in cities. However, some universities and cities are still fragmented, and popular science tourism is one way to solve this. The purposes and destinations of popular science tourism differ from those of traditional tourism. Consequently, their resources have a high development value for ecological and human resources. However, research on popular science tourism in China is still in its infancy. Here, we studied popular science tourism from a geographical perspective, selecting scale capacity, environmental level, resource level, location, and service conditions as factors to construct a judgment matrix to calculate the weights of indicators at various levels. Analytic hierarchy was used to build a popular science tourism evaluation system for Guangzhou University Town as a case study and the suitability of each tourist destination in the university town was evaluated. The results show that west Guangzhou University Town is more suitable for popular science tourism than the east, which possesses more value for popular science tourism development. Finally, we give recommendations for developing popular science tourism in Guangzhou University Town.
Real Options Analysis for Land and Water Solar Deployment in Idle Areas of Agricultural Dam: A Case Study of South Korea
Authors: Na Seoungbeom , Kim Kyeongseok , Jang Woosik , Lee Changgeun
Published: February 2022

This study presents a real options-based framework for investment in land and water solar power projects in the idle areas of agricultural dams. The following four-step framework was verified through a case study conducted in South Korea: (1) select the location and size of the project; (2) define uncertainties in the project data (construction cost, O&M cost, sunshine hours, and mechanical efficiency) and market data (inflation, discount rate, risk-free interest rate, and electricity selling price); (3) estimate cash flow and project volatility to calculate the option value using a binomial tree model; and (4) make decisions regarding project investment. A case study was conducted for the Naju Agricultural Dam (NAD) project, which has a net present value of −6.67 million USD, but will have a profit of 38.17 million USD after an abandonment option is applied using the proposed framework. Our contributions provide a framework for evaluating the economic feasibility of installing solar projects in idle areas of agricultural dams and for improving the profitability of solar projects using the abandonment option. The proposed framework can assist investors in planning solar projects in idle areas of agricultural dams.
Reducing the Environmental Impacts of Desalination Reject Brine Using Modified Solvay Process Based on Calcium Oxide
Authors: Setayeshmanesh Tahereh , Parivazh Mohammad Mehdi , Abbasi Mohsen , Osfouri Shahriar , Dianat Mohammad Javad , Akrami Mohammad
Published: February 2022

In this research, the influence of a variety of operational factors such as the temperature of the reaction, gas flow rate, concentration of NaCl, and the amount of Ca(OH)2 for reducing the environmental impacts of desalination reject brine using the calcium oxide-based modified Solvay process were investigated. For this purpose, response surface modeling (RSM) and central composite design (CCD) were applied. The significance of these factors and their interactions was assessed using an analysis of variance (ANOVA) technique with a 95% degree of certainty (p < 0.05). Optimal conditions for this process included: a temperature of 10 °C, a Ca(OH)2/NaCl concentration ratio of 0.36, and a gas flow rate of 800 mL/min. Under these conditions, the maximum sodium removal efficiency from the synthetic sodium chloride solution was 53.51%. Subsequently, by employing the real brine rejected from the desalination unit with a 63 g/L salinity level under optimal conditions, the removal rate of sodium up to 43% was achieved. To investigate the process’s kinetics of Na elimination, three different kinds of kinetics models were applied from zero to second order. R squared values of 0.9101, 0.915, and 0.9141 were obtained in this investigation for zero-, first-, and second-degree kinetic models, respectively, when synthetic reject saline reacted. In contrast, according to R squared’s results with utilizing real rejected brine, the results for the model of kinetics were: R squared = 0.9115, 0.9324, and 0.9532, correspondingly. As a result, the elimination of sodium from real reject brine is consistent with the second-order kinetic model. According to the findings, the calcium oxide-based modified Solvay method offers a great deal of promise for desalination of brine rejected from desalination units and reducing their environmental impacts. The primary benefit of this technology is producing a usable solid product (sodium bicarbonate) from sodium chloride in the brine solution.
Lighting in Kindergartens: Towards Innovative Design Concepts for Lighting Design in Kindergartens Based on Children’s Perception of Space
Authors: Angelaki Stavroula , Triantafyllidis Georgios A. , Besenecker Ute
Published: February 2022

Light is the foundation of the visual perceptual process that initiates the evaluation of the surrounding area. Linked to various aspects and rhythms of the body, light connects us to both the natural environment and the interior space. The process of perceiving and assessing space for children and adults with different viewing heights and viewing perspectives as well as the role of light to facilitate this are the key elements of this study. The paper describes general lighting design strategies for kindergartens, specifically developed to create an environment that takes into account the children’s scale and cognitive processes. The objective was to discuss environments that support the child’s spatial perception along with shape and object recognition by means of lighting design, for example by creating distinct, well-placed shadows. The proposed strategies are informed by a literature review on the concepts and interrelations of light, human physiology and the perception of scale and space. In addition, our process also included visits to kindergartens to observe and analyse existing lighting as well as the use of simulation programs to test lighting scenarios and their patterns/distribution of light and shadow. The outcome described in this paper is a proposal and strategy to take into account children’s vantage points when designing lighting in kindergartens that is still open to practical interpretations in real-world sites.
The Ethical Governance for the Vulnerability of Care Robots: Interactive-Distance-Oriented Flexible Design
Authors: Zhang Zhengqing , Zhang Chenggang , Li Xiaomeng
Published: February 2022

The application of caring robots is currently a widely accepted solution to the problem of aging. However, for the elderly groups who live in gregarious residences and share intelligence devices, caring robots will cause intimacy and assistance dilemmas in the relationship between humans and non-human agencies. This is an information-assisted machine setting, with resulting design ethics issues brought about by the binary values of human and machine, body and mind. The “vulnerability” in risk ethics demonstrates that the ethical problems of human institutions stem from the increase of dependence and the obstruction of intimacy, which are essentially caused by the increased degree of ethical risk exposure and the restriction of agency. Based on value-sensitive design, caring ethics and machine ethics, this paper proposes a flexible design with the interaction-distance-oriented concept, and reprograms the ethical design of caring robots with intentional distance, representational distance and interpretive distance as indicators. The main purpose is to advocate a new type of human-machine interaction relationship emphasizing diversity and physical interaction.
Strategic Allocation of Development Projects in Post-Conflict Regions: A Gender Perspective for Colombia
Authors: Nawrotzki Raphael J. , Gantner Verena , Balzer Jana , Wencker Thomas , Brüntrup-Seidemann Sabine
Published: February 2022

We know little about the general geographic allocation of development projects in post-conflict regions, and specifically of gender-focused projects. In this study, we explore whether donor agencies prefer to work in “safe” places or dare to operate in conflict-affected zones. Using Colombia as a case study, we combine data on battle deaths from the UCDP Georeferenced Event Dataset (1994–2004) with georeferenced information on the location of development projects from the Colombia AIMS dataset (2006–2013) and manually geocode data for German-funded development projects (2012–2018) with gender as a significant objective. Using count models (N = 1120), we find a statistically strong and positive relationship: an increase in battle deaths increases the number of development projects (with and without gender-focus) in a municipality. Interaction models further reveal an amplification of this relationship for regions with a large proportion of female-headed households, as well as a high number of formally employed and literate women. A context-sensitive interpretation of our findings suggests that (1) development projects in general, and German-funded gender-focused projects in particular, dare to operate in post-conflict settings; (2) women may play an active role as community leaders and mobilizers to influence the allocation of development programs to certain regions.
Can Senior Travelers Save Japanese Hot Springs? A Psychographic Segmentation of Visitors and Their Intention to Visit Onsen Establishments during COVID-19
Authors: Handler Isabell
Published: February 2022

To develop tourism products and campaigns that will not just help Japanese hot springs survive but revive and sustain them in the future, it is imperative to understand the behavioral intentions of its visitors during the coronavirus disease (COVID-19) pandemic. This study aims to investigate how the important market of senior Japanese visitors is characterized by their attitudes and feelings toward visiting hot springs during the COVID-19 pandemic. In addition, it explores whether external travel incentives can increase seniors’ intention to visit. A market research study was conducted on Japanese nationals, using 507 questionnaires. A factor analysis generated five attitude dimensions: (1) response efficacy, (2) perceived COVID-19 infectability, (3) self-efficacy, (4) crowding perception and feeling, and (5) perceived threat severity. Through k-means cluster analysis, the segmentation produced three distinct tourist segments: trusting seniors, concerned seniors, and indifferent seniors. Each segment is clearly described in terms of attitudes, considering distinct sociodemographic characteristics. Practical suggestions on managing and targeting these segments are also discussed.
An Evaluation of the Impact of Databases on End-of-Life Embodied Carbon Estimation
Authors: Blay-Armah Augustine , Bahadori-Jahromi Ali , Mylona Anastasia , Barthorpe Mark , Ferri Marco
Published: February 2022

The growing awareness of the need to minimise greenhouse gas (GHG) and mitigate climate change has resulted in a greater focus on the embodied carbon (EC) of construction material. One way to ensure the environmental impact of building activities is minimised to a reasonable level is the calculation of their EC. Whilst there are a few studies investigating the role of embodied carbon factor (ECF) databases on the accuracy of EC calculation from cradle to gate, very little is known about the impact of different databases on the end-of-life (EoL) EC calculation. Using ECFs derived from the UK Department for Business, Energy and Industrial Strategy (BEIS), the Royal Institute of Chartered Surveyors (RICS) default values and the Institution of Structural Engineers (IStructE) suggested percentages for different elements of a building’s lifecycle stages, this study presents the impact of different data sources on the calculation of EoL EC. The study revealed that a lack of EoL ECFs databases could result in a significant difference of about 61% and 141% in the calculation of EC.
Profit-Sharing Contracts for Fresh Agricultural Products Supply Chain Considering Spatio-Temporal Costs
Authors: Li Min , He Lina , Yang Guangchuan , Lian Zhen
Published: February 2022

This paper investigated the effects of the informational asymmetry phenomenon that occurs in the direct sale of fresh agricultural products (FAP) in an e-commerce environment. A three-level FAP supply chain was proposed, which was composed of a FAP supplier, a logistics service provider, and a large e-commerce platform. Considering the perishable nature of FAP, this paper analyzed the effects of logistics spatio-temporal costs and the freshness of FAP on the profit of each stakeholder in the supply chain. Three scenarios were considered: (1) complete information, (2) partial information, and (3) considering logistics spatio-temporal cost. Analytical models were developed based on the principal-agent theory and the supply chain coordination contract theory to depict the effects of a profit-sharing contract on the operations of the FAP supply chain. Modeling results indicated that under a complete information condition, an increase in the loss rate of FAP correlated to a decrease in the profit of the FAP supply chain. Under a partial information condition, considering the loss rate of FAP and the potential compensation costs to suppliers, when the loss rate of FAP was fixed, the profit of each stakeholder in the FAP supply chain displayed a decreasing trend in relation to compensation ratio. In comparison, when the compensation ratio was fixed, the total profit decreased as the freshness of the FAP degraded. To improve customer satisfaction, this paper recommends adding a front warehouse to improve the freshness of FAP. Although this option increases the logistics costs, it has the potential of increasing the overall profit of the FAP supply chain. Findings from this research have the potential to help the e-commerce platform with coordinating the various stakeholders on the supply chain to determine the optimal quality and quantity of FAPs, eventually improving the operational efficiency of the FAP direct sales supply chain by reducing the logistics costs of FAP.
Don’t Split Them Up! Landscape Design of Multifunctional Open Spaces Suitable for Coping with Flash Floods and River Floods
Authors: Paolinelli Gabriele , Cei Marco , Cristiani Nicoletta , Marinaro Ludovica , Veronesi Flavia
Published: February 2022

Pressures arising from agriculture, infrastructures and settlements have gradually reduced natural spaces of European watercourses limiting their self-regulation capacities, environmental and social potentials, resulting in widespread critical anthropic features. Dealing with flood phenomena adds artificiality, as several works for hydraulic protection are necessary. This was the case of Pistoia, a small city in the north of Tuscany, where the Ombrone stream, held in a straight-channeled course since the 18th century, sometimes breaks its embankments and floods the low plain from the southeast of Pistoia to downstream. Complying with the EU Floods Directive (2007/60/EC), the regional authority for flood risks planned some basins in the high plain upstream of Pistoia. A study we developed before this research assumed to shift the design approach from functional separation to full integration of hydraulic works in an area planned as an urban park for several years, but still in waiting. We now carried out a second study that adopts the concept of deep structure as the main design reference to “see” the park in the landscape features. This article concerns the research by the design process just developed to investigate a sustainable layout of the place new hydraulic asset as a basic landscape identity of the future park. Not to split spaces up with regard to their main functions was the general aim the process was focused on to combine an effective hydraulic protection with a full environmental and social enhancement of the urban park.
Special Issue on Safe and Reliable AI for Smart Sustainable Cities
Authors: Izonin Ivan , Chretien Stephane , Ebrahimnejad Ali
Published: February 2022

Today, most of the world’s inhabitants live in cities [...]
New Progressive Iterative Approximation Techniques for Shepard-Type Curves
Authors: Amato Umberto , Della Vecchia Biancamaria
Published: February 2022

Progressive iterative approximation (PIA) technique is an efficient and intuitive method for data fitting. In CAGD modeling, if the given data points are taken as initial control points, PIA process generates a series of shaping curves by adjusting the control points iteratively, while the limit curve interpolates the data points. Such format was used successfully for Shepard-type curves. The aim of the paper is to construct simple variants of the PIA method for Shepard-type curves producing novel curves modeling data points, so the designer can choose among several pencils of shapes outlining original control polygon. Matrix formulations, convergence results, error estimates, algorithmic formulations, critical comparisons, and numerical tests are shown. An application to a progressive modeling format by truncated wavelet transform is also presented, improving in some sense analogous process by truncated Fourier transform. By playing on two shapes handles—the number of base wavelet transform functions and the iteration level of PIA algorithm—several new contours modeling the given control points are constructed.
Application of Lie Symmetry to a Mathematical Model that Describes a Cancer Sub-Network
Authors: Matadi Maba Boniface
Published: February 2022

In this paper, a mathematical model of a cancer sub-network is analysed from the view point of Lie symmetry methods. This model discusses a human cancer cell which is developed due to the dysfunction of some genes at the R-checkpoint during the cell cycle. The primary purpose of this paper is to apply the techniques of Lie symmetry to the model and present some approximated solutions for the three-dimensional system of first-order ordinary differential equations describing a cancer sub-network. The result shows that the phosphatase gene (Cdc25A) regulates the cyclin-dependent kinases inhibitor (P27Kip1). Furthermore, this research discovered that the activity that reverses the inhibitory effects on cell cycle progression at the R-checkpoint initiates a pathway.
Recombinant Mosquito Densovirus with Bti Toxins Significantly Improves Pathogenicity against Aedes albopictus
Authors: Batool Khadija , Alam Intikhab , Liu Peiwen , Shu Zeng , Zhao Siyu , Yang Wenqiang , Jie Xiao , Gu Jinbao , Chen Xiao-Guang
Published: February 2022

Mosquito densoviruses (MDVs) are mosquito-specific viruses that are recommended as mosquito bio-control agents. The MDV Aedes aegypti densovirus (AeDNV) is a good candidate for controlling mosquitoes. However, the slow activity restricts their widespread use for vector control. In this study, we introduced the Bacillus thuringiensis (Bti) toxin Cry11Aa domain II loop α8 and Cyt1Aa loop β6-αE peptides into the AeDNV genome to improve its mosquitocidal efficiency; protein expression was confirmed using nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS). Recombinant plasmids were transfected into mosquito C6/36 cell lines, and the expression of specific peptides was detected through RT-PCR. A toxicity bioassay against the first instar Aedes albopictus larvae revealed that the pathogenic activity of recombinant AeDNV was significantly higher and faster than the wild-type (wt) viruses, and mortality increased in a dose-dependent manner. The recombinant viruses were genetically stable and displayed growth phenotype and virus proliferation ability, similar to wild-type AeDNV. Our novel results offer further insights by combining two mosquitocidal pathogens to improve viral toxicity for mosquito control.
The RADIOSTAR Project
Authors: Lugaro Maria , Côté Benoit , Pignatari Marco , Yagüe López Andrés , Brinkman Hannah , Cseh Borbála , Den Hartogh Jacqueline , Doherty Carolyn Louise , Karakas Amanda Irene , Kobayashi Chiaki , Lawson Thomas , Pető Mária , Soós Benjámin , Trueman Thomas , Világos Blanka
Published: February 2022

Radioactive nuclei are the key to understanding the circumstances of the birth of our Sun because meteoritic analysis has proven that many of them were present at that time. Their origin, however, has been so far elusive. The ERC-CoG-2016 RADIOSTAR project is dedicated to investigating the production of radioactive nuclei by nuclear reactions inside stars, their evolution in the Milky Way Galaxy, and their presence in molecular clouds. So far, we have discovered that: (i) radioactive nuclei produced by slow (107Pd and 182Hf) and rapid (129I and 247Cm) neutron captures originated from stellar sources —asymptotic giant branch (AGB) stars and compact binary mergers, respectively—within the galactic environment that predated the formation of the molecular cloud where the Sun was born; (ii) the time that elapsed from the birth of the cloud to the birth of the Sun was of the order of 107 years, and (iii) the abundances of the very short-lived nuclei 26Al, 36Cl, and 41Ca can be explained by massive star winds in single or binary systems, if these winds directly polluted the early Solar System. Our current and future work, as required to finalise the picture of the origin of radioactive nuclei in the Solar System, involves studying the possible origin of radioactive nuclei in the early Solar System from core-collapse supernovae, investigating the production of 107Pd in massive star winds, modelling the transport and mixing of radioactive nuclei in the galactic and molecular cloud medium, and calculating the galactic chemical evolution of 53Mn and 60Fe and of the p-process isotopes 92Nb and 146Sm.
THαβ Immunological Pathway as Protective Immune Response against Prion Diseases: An Insight for Prion Infection Therapy
Authors: Tsou Adam , Chen Po-Jui , Tsai Kuo-Wang , Hu Wan-Chung , Lu Kuo-Cheng
Published: February 2022

Prion diseases, including Creutzfeldt–Jakob disease, are mediated by transmissible proteinaceous pathogens. Pathological changes indicative of neuro-degeneration have been observed in the brains of affected patients. Simultaneously, microglial activation, along with the upregulation of pro-inflammatory cytokines, including IL-1 or TNF-α, have also been observed in brain tissue of these patients. Consequently, pro-inflammatory cytokines are thought to be involved in the pathogenesis of these diseases. Accelerated prion infections have been seen in interleukin-10 knockout mice, and type 1 interferons have been found to be protective against these diseases. Since interleukin-10 and type 1 interferons are key mediators of the antiviral THαβ immunological pathway, protective host immunity against prion diseases may be regulated via THαβ immunity. Currently no effective treatment strategies exist for prion disease; however, drugs that target the regulation of IL-10, IFN-alpha, or IFN-β, and consequently modulate the THαβ immunological pathway, may prove to be effective therapeutic options.
Monocytes in HIV and SIV Infection and Aging: Implications for Inflamm-Aging and Accelerated Aging
Authors: Wallis Zoey K. , Williams Kenneth C.
Published: February 2022

Before the antiretroviral therapy (ART) era, people living with HIV (PLWH) experienced complications due to AIDS more so than aging. With ART and the extended lifespan of PLWH, HIV comorbidities also include aging—most likely due to accelerated aging—as well as a cardiovascular, neurocognitive disorders, lung and kidney disease, and malignancies. The broad evidence suggests that HIV with ART is associated with accentuated aging, and that the age-related comorbidities occur earlier, due in part to chronic immune activation, co-infections, and possibly the effects of ART alone. Normally the immune system undergoes alterations of lymphocyte and monocyte populations with aging, that include diminished naïve T- and B-lymphocyte numbers, a reliance on memory lymphocytes, and a skewed production of myeloid cells leading to age-related inflammation, termed “inflamm-aging”. Specifically, absolute numbers and relative proportions of monocytes and monocyte subpopulations are skewed with age along with myeloid mitochondrial dysfunction, resulting in increased accumulation of reactive oxygen species (ROS). Additionally, an increase in biomarkers of myeloid activation (IL-6, sCD14, and sCD163) occurs with chronic HIV infection and with age, and may contribute to immunosenescence. Chronic HIV infection accelerates aging; meanwhile, ART treatment may slow age-related acceleration, but is not sufficient to stop aging or age-related comorbidities. Overall, a better understanding of the mechanisms behind accentuated aging with HIV and the effects of myeloid activation and turnover is needed for future therapies.
Comparative Investigation of Methods for Analysis of SARS-CoV-2-Spike-Specific Antisera
Authors: Herrlein Marie-Luise , Hein Sascha , Zahn Tobias , Mhedhbi Ines , Raupach Jan , Husria Younes , Benz Nuka Ivalu , Eisert Jonathan , Bender Daniela , Haberger Vanessa , Hastert Florian D. , Henss Lisa , Schnierle Barbara S. , Stingl Julia C. , Dreher Michael , Hildt Eberhard
Published: February 2022

In light of an increasing number of vaccinated and convalescent individuals, there is a major need for the development of robust methods for the quantification of neutralizing antibodies; although, a defined correlate of protection is still missing. Sera from hospitalized COVID-19 patients suffering or not suffering from acute respiratory distress syndrome (ARDS) were comparatively analyzed by plaque reduction neutralization test (PRNT) and pseudotype-based neutralization assays to quantify their neutralizing capacity. The two neutralization assays showed comparable data. In case of the non-ARDS sera, there was a distinct correlation between the data from the neutralization assays on the one hand, and enzyme-linked immune sorbent assay (ELISA), as well as biophysical analyses, on the other hand. As such, surface plasmon resonance (SPR)-based assays for quantification of binding antibodies or analysis of the stability of the antigen–antibody interaction and inhibition of syncytium formation, determined by cell fusion assays, were performed. In the case of ARDS sera, which are characterized by a significantly higher fraction of RBD-binding IgA antibodies, there is a clear correlation between the neutralization assays and the ELISA data. In contrast to this, a less clear correlation between the biophysical analyses on the one hand and ELISAs and neutralization assays on the other hand was observed, which might be explained by the heterogeneity of the antibodies. To conclude, for less complex immune sera—as in cases of non-ARDS sera—combinations of titer quantification by ELISA with inhibition of syncytium formation, SPR-based analysis of antibody binding, determination of the stability of the antigen–antibody complex, and competition of the RBD-ACE2 binding represent alternatives to the classic PRNT for analysis of the neutralizing potential of SARS-CoV-2-specific sera, without the requirement for a BSL3 facility.
Elephant Endotheliotropic Herpesvirus 1, 4 and 5 in China: Occurrence in Multiple Sample Types and Implications for Wild and Captive Population Surveillance
Authors: Yang Nian , Bao Mingwei , Zhu Biru , Shen Qingzhong , Guo Xianming , Li Wenwen , Tang Ruchun , Zhu Di , Tang Yinpu , Phalen David N. , Zhang Li
Published: February 2022

Elephant endotheliotropic herpesviruses (EEHVs) are important causes of death in both captive and wild Asian elephants (Elephas maximus). Nothing is known about the prevalence of EEHVs in wild or domestic elephants in China. To determine if EEHVs are present in elephants in China, 126 wild elephants from three populations and 202 captive individuals from zoos (n = 155) and the Wild Elephant Valley (n = 47) were screened using semi-nested polymerase chain reaction assays with EEHV-redundant and EEHV1/4/5-specific primers. EEHV1B and EEHV4 were detected in samples from both wild (EEHV1B:8/126; EEHV4:2/126) and captive (EEHV1B:5/155; EEHV4:9/155) elephants, while EEHV1A (six cases) and EEHV5 (one case) were only present in the captive elephants from the Wild Elephant Valley. EEHV1 was detected in blood and trunk and oral swabs; EEHV4 was detected in trunk and oral swabs as well as feces; EEHV5 was found in trunk and oral swabs. No significant age or sex association with EEHV1A, EEHV1B, or EEHV5 positivity was observed. An age association with EEHV4 positivity was found, with all unweaned elephants being EEHV4 positive, but an association with the sex of the elephant was not observed. These findings represent the first documentation of EEHV presence in captive and wild elephants in China. These findings also document EEHV1B and EEHV4 shedding in feces and demonstrate the utility of fecal screening as a tool for investigating EEHV4 infection in wild populations of elephants. It is recommended that EEHV testing be included in surveillance programs for captive and wild elephants in China.
Improved Subtyping of Avian Influenza Viruses Using an RT-qPCR-Based Low Density Array: ‘Riems Influenza a Typing Array’, Version 2 (RITA-2)
Authors: Hassan Kareem E. , Ahrens Ann Kathrin , Ali Ahmed , El-Kady Magdy F. , Hafez Hafez M. , Mettenleiter Thomas C. , Beer Martin , Harder Timm
Published: February 2022

Avian influenza virus (AIV) variants emerge frequently, which challenges rapid diagnosis. Appropriate diagnosis reaching the sub- and pathotype level is the basis of combatting notifiable AIV infections. Real-time RT-PCR (RT-qPCR) has become a standard diagnostic tool. Here, a total of 24 arrayed RT-qPCRs is introduced for full subtyping of 16 hemagglutinin and nine neuraminidase subtypes of AIV. This array, designated Riems Influenza A Typing Array version 2 (RITA-2), represents an updated and economized version of the RITA-1 array previously published by Hoffmann et al. RITA-2 provides improved integration of assays (24 instead of 32 parallel reactions) and reduced assay volume (12.5 µL). The technique also adds RT-qPCRs to detect Newcastle Disease (NDV) and Infectious Bronchitis viruses (IBV). In addition, it maximizes inclusivity (all sequences within one subtype) and exclusivity (no intersubtypic cross-reactions) as shown in validation runs using a panel of 428 AIV reference isolates, 15 reference samples each of NDV and IBV, and 122 clinical samples. The open format of RITA-2 is particularly tailored to subtyping influenza A virus of avian hosts and Eurasian geographic origin. Decoupling and re-arranging selected RT-qPCRs to detect specific AIV variants causing epizootic outbreaks with a temporal and/or geographic restriction is possible.
West Nile and Usutu Virus Introduction via Migratory Birds: A Retrospective Analysis in Italy
Authors: Mancuso Elisa , Cecere Jacopo Giuseppe , Iapaolo Federica , Di Gennaro Annapia , Sacchi Massimo , Savini Giovanni , Spina Fernando , Monaco Federica
Published: February 2022

The actual contribution of migratory birds in spreading West Nile (WNV) and Usutu virus (USUV) across Europe and from Africa to old countries is still controversial. In this study, we reported the results of molecular and serological surveys on migrating birds sampled during peaks of spring and autumn migration at 11 Italian sites located along important flyways, from 2012 to 2014. A total of 1335 specimens made of individual or pooled sera, and organs from 275 dead birds were tested for WNV and USUV RNA by real time PCR (RT-PCR). Furthermore, sera were tested by serum neutralization assay for detecting WNV and USUV neutralizing antibodies. Molecular tests detected WNV lineage 2 RNA in a pool made of three Song Thrush (Turdus philomelos) sera sampled in autumn, and lineage 1 in kidneys of six trans-Saharan birds sampled in spring. Neutralizing antibodies against WNV and USUV were found in 5.80% (n = 72; 17 bird species) and 0.32% (n = 4; 4 bird species) of the tested sera, respectively. Our results do not exclude the role of migratory birds as potential spreaders of WNV and USUV from Africa and Central Europe to Mediterranean areas and highlight the importance of a more extensive active surveillance of zoonotic viruses.
Structural Bases of Zoonotic and Zooanthroponotic Transmission of SARS-CoV-2
Authors: Clayton Emily , Ackerley Jacob , Aelmans Marianne , Ali Noor , Ashcroft Zoe , Ashton Clara , Barker Robert , Budryte Vakare , Burrows Callum , Cai Shanshan , Callaghan Alex , Carberry Jake , Chatwin Rebecca , Davies Isabella , Farlow Chloe , Gamblin Samuel , Iacobut Aida , Lambe Adam , Lynch Francesca , Mihalache Diana , Mokbel Amani , Potamsetty Santosh , Qadir Zara , Soden Jack , Sun Xiaohan , Vasile Alexandru , Wheeler Otto , Rohaim Mohammed A. , Munir Muhammad
Published: February 2022

The emergence of multiple variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) highlights the importance of possible animal-to-human (zoonotic) and human-to-animal (zooanthroponotic) transmission and potential spread within animal species. A range of animal species have been verified for SARS-CoV-2 susceptibility, either in vitro or in vivo. However, the molecular bases of such a broad host spectrum for the SARS-CoV-2 remains elusive. Here, we structurally and genetically analysed the interaction between the spike protein, with a particular focus on receptor binding domains (RBDs), of SARS-CoV-2 and its receptor angiotensin-converting enzyme 2 (ACE2) for all conceivably susceptible groups of animals to gauge the structural bases of the SARS-CoV-2 host spectrum. We describe our findings in the context of existing animal infection-based models to provide a foundation on the possible virus persistence in animals and their implications in the future eradication of COVID-19.
How RSV Proteins Join Forces to Overcome the Host Innate Immune Response
Authors: Van Royen Tessa , Rossey Iebe , Sedeyn Koen , Schepens Bert , Saelens Xavier
Published: February 2022

Respiratory syncytial virus (RSV) is the leading cause of severe acute lower respiratory tract infections in infants worldwide. Although several pattern recognition receptors (PRRs) can sense RSV-derived pathogen-associated molecular patterns (PAMPs), infection with RSV is typically associated with low to undetectable levels of type I interferons (IFNs). Multiple RSV proteins can hinder the host’s innate immune response. The main players are NS1 and NS2 which suppress type I IFN production and signalling in multiple ways. The recruitment of innate immune cells and the production of several cytokines are reduced by RSV G. Next, RSV N can sequester immunostimulatory proteins to inclusion bodies (IBs). N might also facilitate the assembly of a multiprotein complex that is responsible for the negative regulation of innate immune pathways. Furthermore, RSV M modulates the host’s innate immune response. The nuclear accumulation of RSV M has been linked to an impaired host gene transcription, in particular for nuclear-encoded mitochondrial proteins. In addition, RSV M might also directly target mitochondrial proteins which results in a reduced mitochondrion-mediated innate immune recognition of RSV. Lastly, RSV SH might prolong the viral replication in infected cells and influence cytokine production.
A Comparison with Adverse Events Following Immunization Associated with Sabin-Strains and Salk-Strains Inactivated Polio Vaccines in Zhejiang Province, China
Authors: Lv Huakun , Pan Xuejiao , Liang Hui , Chen Yaping , Wang Ying , Chen Fuxing , Shen Linzhi , Hu Yu
Published: February 2022

Objectives: One dose of Sabin-strains inactivated polio vaccine (IPV) was introduced into the Chinese immunization program on 1 May 2016. This study aimed to evaluate the safety of Sabin-strains IPV and provide a comparison with conventional Salk-strains IPV. Methods: Adverse events following immunization (AEFI) records associated with Sabin-strains IPV and Salk-strains IPV were extracted from the national AEFI surveillance system (NAEFISS) from 1 May 2016 to 31 December 2020. The vaccination information on Sabin-strains IPV and Salk-strains IPV during the same period was obtained from the Zhejiang provincial immunization information system. Reporting rates of AEFI were calculated by age, city, severity of AEFI, categories of AEFI, and reaction categories and were compared between Sabin-strains IPV and Salk-strains IPV. Results: In total, 3,861,758 doses of Sabin-strains IPV and 1,018,604 doses of Salk-strains IPV were administered during the study period. The overall AEFI reporting rate for Sabin-strains IPV (3.96/10,000 doses) was significantly lower than that for Salk-strains IPV (5.03/10,000 doses) due to the reporting rate of the minor vaccine product-related reaction following Sabin-strains IPV was significantly lower than that for Salk-strains IPV (2.76/10,000 doses vs. 3.83/10,000 doses). The most frequently reported symptoms/signs were fever, induration/swelling, and rash/urticaria. The most frequently reported serious AEFI with a causal relationship was febrile convulsion, with the reporting rates of 0.10/10,000 doses for Sabin-strains IPV and 0.08/10,000 doses for Salk-strains IPV. No significant difference was found in the reporting rates of the other serious AEFI between the two types of IPV. Conclusion: Most of the AEFI following Sabin-strains IPV and Salk-strains IPV were mild and common adverse reactions. The reporting rate of serious AEFI was not significantly different between Sabin-strains IPV and Salk-strains IPV. Sabin-strains IPV had a favorable safety profile and could be widely used.
Rodent–Human Interface: Behavioral Risk Factors and Leptospirosis in a Province in the Central Region of Thailand
Authors: Suwannarong Kanokwan , Soonthornworasiri Ngamphol , Maneekan Pannamas , Yimsamran Surapon , Balthip Karnsunaphat , Maneewatchararangsri Santi , Saisongkorh Watcharee , Saengkul Chutarat , Sangmukdanun Suntaree , Phunta Nittaya , Singhasivanon Pratap
Published: February 2022

This sequential explanatory mixed-method study consisted of analytical, cross-sectional, and qualitative studies. The research was conducted in the Khao Nor and Khao Kaew areas of the Banphot Pisai districts of Nakhon Sawan Province in 2019. Here, we examined the rodent contact characteristics of villagers in these areas and determined the potential characteristics/risk factors associated with rodents using a semi-structured questionnaire, key informant interview (KII), and focus group discussion (FGD). Results of the quantitative study (N1 = 372) characterized participants that contacted rodents per gender, age, occupation, knowledge, attitude, and practice (KAP), including their cultural contexts, and beliefs. Ninety participants (24.2%) reported contact with rodents, and the reasons for their direct physical rodent contact were hunting (35, 9.4%), killing (41, 11.0%), preparing rodents as food (33, 8.9%), consuming cooked meats (12, 3.2%), feeding food (4, 1.1%), cleaning feces (17, 4.6%), and cleaning carcasses (33, 8.9%). Moreover, logistic regression results showed that males encountering rodents were statistically significant (Adjusted OR = 3.137, 95% CI 1.914–5.139, p < 0.001). Low monthly household income (<THB 15,000 or <USD 450) was also negatively statistically significant with encountering rodents (Adjusted OR = 0.57, 95% CI 0.33–0.99, p = 0.04). Additionally, the villagers had a low level of knowledge toward zoonotic diseases and inappropriate attitudes and practices toward contacting rodents and zoonotic diseases. Thirty-five qualitative study participants (N2) participated in the KIIs and FGDs. Various rodent contact activities were also reported among the qualitative research participants, such as hunting, consumption, and selling them to their friends and neighbors. However, these rodents also destroyed their belongings, crops, and plants. Some participants also reported that rodents accounted for leptospirosis transmission. As a result, communication intervention should be planned to provide appropriate knowledge and attitude to the villagers, especially among those who have close contact with rodents in the understudied area.
A Hybrid Model for Water Quality Prediction Based on an Artificial Neural Network, Wavelet Transform, and Long Short-Term Memory
Authors: Wu Junhao , Wang Zhaocai
Published: February 2022

Clean water is an indispensable essential resource on which humans and other living beings depend. Therefore, the establishment of a water quality prediction model to predict future water quality conditions has a significant social and economic value. In this study, a model based on an artificial neural network (ANN), discrete wavelet transform (DWT), and long short-term memory (LSTM) was constructed to predict the water quality of the Jinjiang River. Firstly, a multi-layer perceptron neural network was used to process the missing values based on the time series in the water quality dataset used in this research. Secondly, the Daubechies 5 (Db5) wavelet was used to divide the water quality data into low-frequency signals and high-frequency signals. Then, the signals were used as the input of LSTM, and LSTM was used for training, testing, and prediction. Finally, the prediction results were compared with the nonlinear auto regression (NAR) neural network model, the ANN-LSTM model, the ARIMA model, multi-layer perceptron neural networks, the LSTM model, and the CNN-LSTM model. The outcome indicated that the ANN-WT-LSTM model proposed in this study performed better than previous models in many evaluation indices. Therefore, the research methods of this study can provide technical support and practical reference for water quality monitoring and the management of the Jinjiang River and other basins.
Assessment of Groundwater Flow Dynamics Using MODFLOW in Shallow Aquifer System of Mahanadi Delta (East Coast), India
Authors: Behera Ajit Kumar , Pradhan Rudra Mohan , Kumar Sudhir , Chakrapani Govind Joseph , Kumar Pankaj
Published: February 2022

Despite being a biodiversity hotspot, the Mahanadi delta is facing groundwater salinization as one of the main environmental threats in the recent past. Hence, this study attempts to understand the dynamics of groundwater and its sustainable management options through numerical simulation in the Jagatsinghpur deltaic region. The result shows that groundwater in the study area is extensively abstracted for agricultural activities, which also causes the depletion of groundwater levels. The hydraulic head value varies from 0.7 to 15 m above mean sea level (MSL) with an average head of 6 m in this low-lying coastal region. The horizontal hydraulic conductivity and the specific yield values in the area are found to vary from 40 to 45 m/day and 0.05 to 0.07, respectively. The study area has been calibrated for two years (2004–2005) by using these parameters, followed by the validation of four years (2006–2009). The calibrated numerical model is used to evaluate the net recharge and groundwater balance in this study area. The interaction between the river and coastal unconfined aquifer system responds differently in different seasons. The net groundwater recharge to the coastal aquifer has been estimated and varies from 247.89 to 262.63 million cubic meters (MCM) in the year 2006–2007. The model further indicates a net outflow of 8.92–9.64 MCM of groundwater into the Bay of Bengal. Further, the outflow to the sea is preventing the seawater ingress into the shallow coastal aquifer system.