bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of High-Intensity Swimming Interval Training on Area, Perimeter, Circularity Index and Phenotype of Cardiac Mitochondrial Ultrastructure in Sprague Dawley Rats
Authors: Pasmiño Grace , Paredes Marco , Silva Héctor
Published: August 2024

Physical inactivity impairs health by increasing morbidity. In childhood, modifiable risk factors associated with cardiovascular pathologies and related to mitochondrial function and structure are initiated by physical inactivity. The objective of this study was to analyze the effect of high-intensity swimming interval training (HIIT-swim) on cardiac mitochondrial ultrastructure in young Sprague Dawley rats compared with a sedentary group. Five-week-old Sprague Dawley rats (n = 18) were divided into a control group (C) (n = 6), a sedentary group (S) (n = 6) and an HIIT-swim group (H-s) (n = 6), the last of which performed HIIT-swim for 4 weeks. A mitochondrial ultrastructural evaluation was performed using transmission electron microscopy. In the H-s rats, mitochondrial areas and perimeters were found to be statistically significantly different from those of the C and S rats. In addition, no predominant intramitochondrial multifragmentation was observed in the mitochondria of H-s rats, but multifragmentation was evident in the mitochondria of S rats.
Corrosion Behavior of 3104 Aluminum Cans When Used as Packaging for Chinese Liquor
Authors: Fan Mingjie , Chen Jinyang , Gu Jie , Wu Zheying
Published: August 2024

Aluminum cans are commonly used for packaging soft drinks and low-alcohol beverages due to their good recyclability. To enhance the economic cycle and expand the packaging of liquors, the feasibility of commercial 3104 aluminum cans for packaging Chinese liquor was studied. The aluminum’s migration into alcoholic solutions was studied using inductively coupled plasma emission spectroscopy (ICP-OES). Electrochemical impedance spectroscopy (EIS) was used to study the corrosion process of epoxy coatings on the aluminum cans. Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), infrared attenuated total reflection (IR-ATR), and X-ray diffraction (XRD) were used to determine the inner coatings and adhering surfaces of the cans and the corrosion process. The results showed that the maximum aluminum migration in Chinese liquor was 4.3572 mg/kg at 60 °C for 30 days. The epoxy coating was corroded enough to decrease the coating impedance and expose the metal substrate after 25 days. Permeation and aging degradation of coatings are the main factors to consider when packaging liquor.
Properties of Polymer-Modified Cement–Water-Glass Slurry
Authors: Yang Qian , Xu Junxiang , Ju Yiheng , Lu Dewang , Meng Wei , Wu Jing , Zhang Xuefu
Published: August 2024

The corrosion resistance of cement–water-glass dual-liquid slurry is poor. Improving its material properties is necessary. In this study, we examined the influence of water-based lotions on the fluidity, gelling time, and mechanical properties of a cement–water-glass dual-liquid slurry based on the mix proportion of the dual-liquid slurry commonly used in construction. The mixture ratio of a C-S (cement–water-glass slurry) dual-liquid slurry was adjusted by introducing a waterborne polyurethane lotion and a waterborne acrylic lotion to modify the traditional C-S dual-liquid slurry material. When acrylic acid is used as a modifying polymer at a dosage of 7.5%, the flowability and gelation time of the dual-liquid slurry are excellent, the compressive strength of the stone body decreases slightly, the flexural strength is improved to a certain extent, and the stone body’s crack resistance during water loss is also enhanced. Moreover, the porosity of the stone body is low.
Compressive Properties and Energy Absorption Characteristics of Co-Continuous Interlocking PDMS/PLA Lattice Composites
Authors: Wang Han , Wang Kedi , Lei Jincheng , Fan Xueling
Published: August 2024

Co-continuous interlocking lattice structures usually present superior compressive properties and energy absorption characteristics. In this study, co-continuous interlocking polydimethylsiloxane/polylactic acid (PDMS/PLA) lattice composites were designed with different strut diameters, and successfully manufactured by combining the fused deposition modeling (FDM) technique and the infiltration method. This fabrication method can realize the change and control of structure parameters. The effects of the strut diameter on the compressive properties and energy absorption behavior of PDMS/PLA lattice composites were investigated by using quasi-static compression tests. The compressive properties of the co-continuous interlocking PDMS/PLA lattice composites can be adjusted in a narrow density range by a linear correlation. The energy absorption density of the co-continuous interlocking PDMS/PLA lattice composites increases with the increase in the PLA strut diameter and presents a higher efficiency peak and wider plateau region. The PLA lattice acts as a skeleton and plays an important role in bearing the compressive load and in energy absorption. The indexes of the compressive properties/energy absorption characteristics and PLA volume fraction of co-continuous interlocking PDMS/PLA lattice composites show linear relationships in logarithmic coordinates. The effect of the PLA volume fraction increasing on the plateau stress is more sensitive than the compressive strength and energy absorption density.
An Asymmetric Independently Steerable Wheel for Climbing Robots and Its Motion Control Method
Authors: Lv Meifeng , Liu Xiaoshun , Xue Lei , Tan Ke , Huang Junhui , Gong Zeyu
Published: August 2024

Climbing robots, with their expansive workspace and flexible deployment modes, have the potential to revolutionize the manufacturing processes of large and complex components. Given that the surfaces to be machined typically exhibit variable curvature, good surface adaptability, load capacity, and motion accuracy are essential prerequisites for climbing robots in manufacturing tasks. This paper addresses the manufacturing requirements of climbing robots by proposing an asymmetric independently steerable wheel (AISW) for climbing robots, along with the motion control method. Firstly, for the adaptability issue of the locomotion mechanism on curved surfaces under heavy load, an asymmetric independently steerable wheel motion module is proposed, which improves the steering difficulty of the traditional independently steerable wheel (ISW) based on the principle of steering assisted by wheels. Secondly, a kinematic model of the AISW chassis is established and, on this basis, a trajectory tracking method based on feedforward and proportional–integral feedback is proposed. Comparative experimental results on large, curved surface components show that the asymmetric independently steerable wheel has lower steering resistance and higher motion accuracy, significantly enhancing the reachability of climbing robots and facilitating their application in the manufacturing of large and complex components.
The Implicit Euler Scheme for FSDEs with Stochastic Forcing: Existence and Uniqueness of the Solution
Authors: Kubilius Kęstutis
Published: August 2024

In this paper, we focus on fractional stochastic differential equations (FSDEs) with a stochastic forcing term, i.e., to FSDE, we add a stochastic forcing term. Using the implicit scheme of Euler’s approximation, the conditions for the existence and uniqueness of the solution of FSDEs with a stochastic forcing term are established. Such equations can be applied to considering FSDEs with a permeable wall.
Multi-Objective Constrained Optimization Model and Molten Iron Allocation Application Based on Hybrid Archimedes Optimization Algorithm
Authors: Hu Huijuan , Shi Shichao , Xu He
Published: August 2024

The challenge of distributing molten iron involves the optimal allocation of blast furnace output to various steelmaking furnaces, considering the blast furnace’s production capacity and the steelmaking converter’s consumption capacity. The primary objective is to prioritize the distribution from the blast furnace to achieve a balance between iron and steel production while ensuring that the volume of hot metal within the system remains within a safe range. To address this, a constrained multi-objective nonlinear programming model is abstracted. A linear weighting method combines multiple objectives into a single objective function, while the Lagrange multiplier method addresses constraints. The proposed hybrid Archimedes optimization algorithm effectively solves this problem, demonstrating significant improvements in time efficiency and precision compared to existing methods.
Quadrature Based Neural Network Learning of Stochastic Hamiltonian Systems
Authors: Cheng Xupeng , Wang Lijin , Cao Yanzhao
Published: August 2024

Hamiltonian Neural Networks (HNNs) provide structure-preserving learning of Hamiltonian systems. In this paper, we extend HNNs to structure-preserving inversion of stochastic Hamiltonian systems (SHSs) from observational data. We propose the quadrature-based models according to the integral form of the SHSs’ solutions, where we denoise the loss-by-moment calculations of the solutions. The integral pattern of the models transforms the source of the essential learning error from the discrepancy between the modified Hamiltonian and the true Hamiltonian in the classical HNN models into that between the integrals and their quadrature approximations. This transforms the challenging task of deriving the relation between the modified and the true Hamiltonians from the (stochastic) Hamilton–Jacobi PDEs, into the one that only requires invoking results from the numerical quadrature theory. Meanwhile, denoising via moments calculations gives a simpler data fitting method than, e.g., via probability density fitting, which may imply better generalization ability in certain circumstances. Numerical experiments validate the proposed learning strategy on several concrete Hamiltonian systems. The experimental results show that both the learned Hamiltonian function and the predicted solution of our quadrature-based model are more accurate than that of the corrected symplectic HNN method on a harmonic oscillator, and the three-point Gaussian quadrature-based model produces higher accuracy in long-time prediction than the Kramers–Moyal method and the numerics-informed likelihood method on the stochastic Kubo oscillator as well as other two stochastic systems with non-polynomial Hamiltonian functions. Moreover, the Hamiltonian learning error εH arising from the Gaussian quadrature-based model is lower than that from Simpson’s quadrature-based model. These demonstrate the superiority of our approach in learning accuracy and long-time prediction ability compared to certain existing methods and exhibit its potential to improve learning accuracy via applying precise quadrature formulae.
Identification of Penexanthone A as a Novel Chemosensitizer to Induce Ferroptosis by Targeting Nrf2 in Human Colorectal Cancer Cells
Authors: Zhao Genshi , Liu Yanying , Wei Xia , Yang Chunxia , Lu Junfei , Yan Shihuan , Ma Xiaolin , Cheng Xue , You Zhengliang , Ding Yue , Guo Hongwei , Su Zhiheng , Xing Shangping , Zhu Dan
Published: August 2024

Ferroptosis has emerged as a potential mechanism for enhancing the efficacy of chemotherapy in cancer treatment. By suppressing nuclear factor erythroid 2-related factor 2 (Nrf2), cancer cells may lose their ability to counteract the oxidative stress induced by chemotherapy, thereby becoming more susceptible to ferroptosis. In this study, we investigate the potential of penexanthone A (PXA), a xanthone dimer component derived from the endophytic fungus Diaporthe goulteri, obtained from mangrove plant Acanthus ilicifolius, to enhance the therapeutic effect of cisplatin (CDDP) on colorectal cancer (CRC) by inhibiting Nrf2. The present study reported that PXA significantly improved the ability of CDDP to inhibit the activity of and induce apoptosis in CRC cells. Moreover, PXA was found to increase the level of oxidative stress and DNA damage caused by CDDP. In addition, the overexpression of Nrf2 reversed the DNA damage and ferroptosis induced by the combination of PXA and CDDP. In vivo experiments using zebrafish xenograft models demonstrated that PXA enhanced the therapeutic effect of CDDP on CRC. These studies suggest that PXA enhanced the sensitivity of CRC to CDDP and induce ferroptosis by targeting Nrf2 inhibition, indicating that PXA might serve as a novel anticancer drug in combination chemotherapy.
Production of Fucoxanthin from Microalgae Isochrysis galbana of Djibouti: Optimization, Correlation with Antioxidant Potential, and Bioinformatics Approaches
Authors: Mohamed Abdoul-Latif Fatouma , Ainane Ayoub , Achenani Laila , Merito Ali Ali , Mohamed Houda , Ali Ahmad , Jutur Pannaga Pavan , Ainane Tarik
Published: August 2024

Fucoxanthin, a carotenoid with remarkable antioxidant properties, has considerable potential for high-value biotechnological applications in the pharmaceutical, nutraceutical, and cosmeceutical fields. However, conventional extraction methods of this molecule from microalgae are limited in terms of cost-effectiveness. This study focused on optimizing biomass and fucoxanthin production from Isochrysis galbana, isolated from the coast of Tadjoura (Djibouti), by testing various culture media. The antioxidant potential of the cultures was evaluated based on the concentrations of fucoxanthin, carotenoids, and total phenols. Different nutrient formulations were tested to determine the optimal combination for a maximum biomass yield. Using the statistical methodology of principal component analysis, Walne and Guillard F/2 media were identified as the most promising, reaching a maximum fucoxanthin yield of 7.8 mg/g. Multiple regression models showed a strong correlation between antioxidant activity and the concentration of fucoxanthin produced. A thorough study of the optimization of I. galbana growth conditions, using a design of experiments, revealed that air flow rate and CO2 flow rate were the most influential factors on fucoxanthin production, reaching a value of 13.4 mg/g. Finally, to validate the antioxidant potential of fucoxanthin, an in silico analysis based on molecular docking was performed, showing that fucoxanthin interacts with antioxidant proteins (3FS1, 3L2C, and 8BBK). This research not only confirmed the positive results of I. galbana cultivation in terms of antioxidant activity, but also provided essential information for the optimization of fucoxanthin production, opening up promising prospects for industrial applications and future research.
Exploring the Influence of Dark Triad and Light Triad Traits on Sport Sciences Students
Authors: Ermis Sermin Agrali , Boyanmis Ayse Hazal , Kesilmiş İnci , Toros Turhan , Ogras Emre Bulent , Akın Manolya , Temel Cenk , Gurkan Alper Cenk , Ocal Yesim Karac
Published: August 2024

Background and Objectives: The primary purpose of the article was to examine the relationship between dark and light personality traits in university students enrolled in the Faculty of Sport Sciences. Materials and Methods: Data were collected from 518 students (208 female, 310 male) with an average age of 20.89 (±2.25). The Short Dark Triad and the Light Triad Scales were used. Harman’s single-factor analysis minimized measurement error, and various statistical methods assessed the effect of gender and age on personality traits. Results: Results indicated a positive correlation among dark personality traits, while light personality traits exhibited more complex relationships. Gender and age were found to significantly influence certain personality traits. Conclusions: This study contributes to the sports literature by exploring the role of demographic variables in personality formation.
Effect of Cr3C2 Addition on Microstructure and Mechanical Properties of WC-CoNiFe Cemented Carbides
Authors: Wu Jinbo , Ren Daping , Xie Bo , He Rongyue , Geng Zhanji , Zhang Zichun , Liu Yang , Wang Dong , Zhu Yanghui , Zhang Wei
Published: August 2024

In traditional cemented carbides, Co is mainly used as a binder. Recently, replacing Co with medium- to high-entropy alloys has shown significant improvements in hardness, fracture toughness, high-temperature oxidation resistance, and corrosion resistance, making it a research focus globally. Both the typical grain refiner Cr3C2 and medium- to high-entropy alloy binders affect the WC grain size in cemented carbides. This study investigates the synergistic grain refinement mechanism of Cr3C2 and medium- to high-entropy alloy binders on WC grains and their impact on the microstructure and mechanical properties of cemented carbides. The results show that increasing the Cr3C2 addition refined WC grains in the WC-CoNiFe alloy, increased coercivity, and enhanced hardness, with transverse rupture strength first increasing and then decreasing. The alloy achieved optimal performance at 0.6 wt.%Cr3C2, with a hardness of 91.25 HRA and transverse rupture strength of 3883.2 MPa.
Effects of Partially Replacing Mo with Nb on the Microstructure and Properties of High-Strength Low-Alloy Steel during Reverse Austenization
Authors: Luo Liang , Zhang Jiajun , Fu Hao , Chen Fuhu , Qin Jianchun , Li Yimin
Published: August 2024

This study investigated the effects of partially replacing expensive Mo with cheaper Nb on the microstructure and properties of high-strength low-alloy (HSLA) steel during reverse austenisation. The mechanical properties of the steel in the hot-rolled state were lower with a partial replacement of Mo by Nb. However, after pre-tempering and reheating and quenching, the strength increased greatly while the ductility and toughness did not decrease much. Thus, the negative effects of replacing Mo with Nb were mostly alleviated, and a good balance between strength, ductility and toughness was achieved. After heat treatment, the mass percentage of precipitates increased substantially, which helped to pin grain boundaries during austenisation. The percent of high-angle grain boundaries greatly increased while the average effective grain size decreased, which improved grain refinement. The results showed that combining a partial replacement of Mo by Nb with heat treatment allows the microstructure and mechanical properties of HSLA steel to be effectively controlled while improving the balance between cost and performance. These findings provide valuable insights into the preparation and design of steels with similar microstructures.
Effects of E-Cigarettes on the Lung and Systemic Metabolome in People with HIV
Authors: Zaparte Aline , Christopher Courtney J. , Arnold Connie , Richey Lauren , Castille Adairre , Mistretta Kyle , Taylor Christopher M. , Lin Huiyi , Nelson Steve , Kirwan John P. , Apolzan John W. , Campagna Shawn R. , Welsh David A.
Published: August 2024

The popularity of e-cigarettes (vaping) has soared, creating a public health crisis among teens and young adults. Chronic vaping can induce gut inflammation and reduce intestinal barrier function through the production of the proinflammatory molecule hydrogen sulfide (H2S). This is particularly concerning for people with HIV (PWH) as they already face impaired immune function and are at a higher risk for metabolic dysregulation, diabetes, and chronic liver disease. Furthermore, PWH experience unhealthy behaviors, making it crucial to understand the systemic metabolic dysregulation and pathophysiological mechanisms associated with vaping in this population. Here, we employed liquid chromatography–mass spectrometry (LC-MS)-based metabolomics to investigate the upper respiratory, circulation, and gut metabolic profiles of PWH who vape (n = 7) and smoke combustible tobacco/marijuana (n = 6) compared to control participants who did not vape or smoke (n = 10). This hypothesis-generating exploratory study revealed systemic alterations in purine, neurotransmitter, and vitamin B metabolisms and tissue-specific changes in inflammatory pathways and cryptic sulfur cycling associated with vaping and combustible tobacco/marijuana smoking in PWH. In addition, this study provides the first link between microbial-derived metabolite 2,3-dihydroxypropane-1-sulfonate (DHPS) and vaping/smoking (tobacco and marijuana)-induced metabolic dyshomeostasis in the gut. These findings highlight the importance of identifying the full biological and clinical significance of the physiological changes and risks associated with vaping.
Metabolic and Oxidative Stress Management Heterogeneity in a Panel of Breast Cancer Cell Lines
Authors: Maycotte Paola , Sarmiento-Salinas Fabiola Lilí , García-Miranda Alin , Ovando-Ovando Cesar Ivan , Robledo-Cadena Diana Xochiquetzal , Hernández-Esquivel Luz , Jasso-Chávez Ricardo , Marín-Hernández Alvaro
Published: August 2024

Metabolic alterations are recognized as one of the hallmarks of cancer. Among these, alterations in mitochondrial function have been associated with an enhanced production of Reactive Oxygen Species (ROS), which activate ROS-regulated cancer cell signaling pathways. Breast cancer is the main cancer-related cause of death for women globally. It is a heterogeneous disease with subtypes characterized by specific molecular features and patient outcomes. With the purpose of identifying differences in energy metabolism and the oxidative stress management system in non-tumorigenic, estrogen receptor positive (ER+) and triple negative (TN) breast cancer cells, we evaluated ROS production, protein enzyme levels and activities and profiled energy metabolism. We found differences in energetic metabolism and ROS management systems between non-tumorigenic and cancer cells and between ER+ and TN breast cancer cells. Our results indicate a dependence on glycolysis despite different glycolytic ATP levels in all cancer cell lines tested. In addition, our data show that high levels of ROS in TN cells are a result of limited antioxidant capacity in the NADPH producing and GSH systems, mitochondrial dysfunction and non-mitochondrial ROS production, making them more sensitive to GSH synthesis inhibitors. Our data suggest that metabolic and antioxidant profiling of breast cancer will provide important targets for metabolic inhibitors or antioxidant treatments for breast cancer therapy.
Replication of Radial Pulses Using Magneto-Rheological Fluids
Authors: Eaton Miranda , Koo Jeong-Hoi , Yang Tae-Heon , Kim Young-Min
Published: August 2024

The radial pulse is a critical health marker with expanding applications in wearable technology. To improve these applications, developing a pulse generator that consistently produces realistic pulses is crucial for validation and training. The goal of this study was to design and test a cost-effective pulse simulator that can accurately replicate a wide range of age-dependent radial pulses with simplicity and precision. To this end, this study incorporated a magneto-rheological (MR) fluid device into a cam-based pulse simulator. The MR device, as a key component, enables pulse shaping without the need for additional cams, substantially reducing the cost and complexity of control compared with existing pulse simulators. To evaluate the performance of the MR pulse simulator, the root-mean-square (RMS) error criterion (less than 5%) was used to compare the experimentally obtained pulse waveform with the in vivo pulse waveform for specific age groups. After demonstrating that the MR simulator could produce three representative in vivo pulses, a parametric study was conducted to show the feasibility of the slope-based pulse-shaping method for the MR pulse simulator to continuously generate a range of age-related pulses.
Application of a Modified First-Order Plate Theory to Structural Analysis of Sensitive Elements in a Pyroelectric Detector
Authors: Lian Mengmeng , Fan Cuiying , Zhan Xiaohan , Zhao Minghao , Qin Guoshuai , Lu Chunsheng
Published: August 2024

Pyroelectric materials, with piezoelectricity and pyroelectricity, have been widely used in infrared thermal detectors. In this paper, a modified first-order plate theory is extended to analyze a pyroelectric sensitive element structure. The displacement, temperature, and electric potential expand along the thickness direction. The governing equation of the pyroelectric plate is built up. The potential distributions with upper and lower electrodes are obtained under different supported boundary conditions. The corresponding numerical results of electric potential are consistent with those obtained by the three-dimensional finite element method. Meanwhile, the theoretical results of electric potential are close to that of experiments. The influence of supported boundary conditions, piezoelectric effect, and plate thickness are analyzed. Numerical results show that the piezoelectric effect reduces the electric potential. The thickness of the pyroelectric plate enhances the electric potential but reduces the response speed of the detector. It is anticipated that the pyroelectric plate theory can provide a theoretical approach for the structural design of pyroelectric sensitive elements.
Identification and Functional Analysis of Novel Long Intergenic RNA in Chicken Macrophages Infected with Avian Pathogenic Escherichia coli
Authors: Ma Yuyi , Cao Xinqi , Sumayya  , Lu Yue , Han Wei , Lamont Susan J. , Sun Hongyan
Published: August 2024

Avian pathogenic E. coli (APEC), a widespread bacterium, results in serious economic losses to the poultry industry annually, and it poses a threat to human health due to the contaminated retail poultry meat and eggs. Recently, it has been demonstrated that long non-coding RNAs played important roles in regulating gene expression and the animal immune response. This study aimed to systematically explore the function of the novel long intergenic non-coding transcript, lincRNA-73240, upon APEC infection. A bioinformatics analysis indicated that lincRNA-73240 had no coding ability and a relative stable secondary structure with multiple hairpin rings. Moreover, the RT-qPCR results showed that lincRNA-73240 was highly expressed in lungs, heart, liver, spleen, cecum tonsils, thymus, ileum, bursa of Fabricius, harderian gland, and muscles in comparison to the cerebrum. Additionally, overexpression of lincRNA-73240 can promote the expression levels of inflammation, apoptosis, autophagy, and oxidative stress-related genes, as well as the production of reactive oxygen species (ROS), malondialdehyde (MDA), and nitric oxide (NO) upon APEC infection, which lead to cellular injury and apoptosis. These findings collectively establish a foundation for the study of the biological function of chicken lincRNA-73240 and provide a theoretical basis for further research on the molecular mechanisms of the chicken immune response.
Bacterial Communities Associated with the Leaves and the Roots of Salt Marsh Plants of Bayfront Beach, Mobile, Alabama, USA
Authors: Majeed Aqsa , Liu Jinbao , Knight Adelle J. , Pajerowska-Mukhtar Karolina M. , Mukhtar M. Shahid
Published: August 2024

Salt marshes are highly dynamic and biologically diverse ecosystems that serve as natural habitats for numerous salt-tolerant plants (halophytes). We investigated the bacterial communities associated with the roots and leaves of plants growing in the coastal salt marshes of the Bayfront Beach, located in Mobile, Alabama, United States. We compared external (epiphytic) and internal (endophytic) communities of both leaf and root plant organs. Using 16S rDNA amplicon sequencing methods, we identified 10 bacterial phyla and 59 different amplicon sequence variants (ASVs) at the genus level. Bacterial strains belonging to the phyla Proteobacteria, Bacteroidetes, and Firmicutes were highly abundant in both leaf and root samples. At the genus level, sequences of the genus Pseudomonas were common across all four sample types, with the highest abundance found in the leaf endophytic community. Additionally, Pantoea was found to be dominant in leaf tissue compared to roots. Our study revealed that plant habitat (internal vs. external for leaves and roots) was a determinant of the bacterial community structure. Co-occurrence network analyses enabled us to discern the intricate characteristics of bacterial taxa. Our network analysis revealed varied levels of ASV complexity in the epiphytic networks of roots and leaves compared to the endophytic networks. Overall, this study advances our understanding of the intricate composition of the bacterial microbiota in habitats (epiphytic and endophytic) and organs (leaf and root) of coastal salt marsh plants and suggests that plants might recruit habitat- and organ-specific bacteria to enhance their tolerance to salt stress.
In Silico Evaluation of Lawsonia intracellularis Genes Orthologous to Genes Associated with Pathogenesis in Other Intracellular Bacteria
Authors: Suarez-Duarte Mirtha E. , Santos Renato L. , Pereira Carlos E. R. , Resende Talita P. , Araujo Matheus D. , Correia Paula A. , Barbosa Jessica C. R. , Laub Ricardo P. , Rodrigues Diego L. N. , Aburjaile Flavia F. , Guedes Roberto M. C.
Published: August 2024

Proliferative enteropathy is an enteric disease caused by the bacterium Lawsonia intracellularis, which affects several species of domestic and wild animals. The mechanisms underlying the mechanisms employed by L. intracellularis to cause host cell proliferation are poorly understood, mostly because this bacterium is extremely difficult to isolate and propagate in vitro. Comparative genomics methods for searching for genes orthologous to genes known to be associated with pathogenesis allow identification of genes potentially involved in pathogenesis by the pathogen of interest. The goal of this study was to carry out in silico research on L. intracellularis genes orthologous to genes required for intracellular invasion and survival present in other pathogenic bacteria, particularly Brucella abortus, B. melitensis, B. suis, Listeria monocytogenes, Mycobacterium tuberculosis, Mycobacterium avium subspecies paratuberculosis, Salmonella enterica, Yersinia pestis, Y. enterocolitica, and Y. pseudotuberculosis. A total of 127 genes associated with invasion and intracellular survival from five known intracellular bacteria were mapped against the predicted proteomes of all L. intracellularis strains publicly available on GenBank, using the OrthoFinder program. A total of 45 L. intracellularis genes were orthologous to genes associated with pathogenesis of other intracellular bacteria. Genes putatively associated with signal the transduction of chemotaxis and cell motility were identified. Genes related to DNA binding and repair were also identified, with some of them supporting a possible association of bacteria with macrophages or inducing pro-inflammatory responses. The homology-based identification of these genes suggests their potential involvement in the virulence and pathogenicity of L. intracellularis, opening avenues for future research and insights into the molecular mechanisms of Lawsonia-elicited proliferative enteropathy.
Prevalence and Characterization of Salmonella during Pork Sausage Manufacturing
Authors: Wottlin Lauren R. , Harvey Roger B. , Norman Keri N. , Droleskey Robert E. , Andrews Kathleen , Jackson Steve J. , Anderson Robin C. , Poole Toni L.
Published: August 2024

Pork carcasses and meat may harbor Salmonella and may contaminate other products during harvest and fabrication. Sources of contamination include manure on hides, environmental contamination, ingredients from external sources, and lymph nodes. Swine lymph nodes are often incorporated into ground meat, as their anatomical location makes removal labor prohibitive. A sausage processing plant in the midwestern United States was sampled monthly (except for December) from May 2021 to April 2022 to enumerate Salmonella and Enterobacteriaceae (EB) throughout the sausage manufacturing process to determine high-risk stages and efficiency of existing in-plant interventions. Salmonella serotypes and antimicrobial susceptibility were evaluated on a subset of isolates recovered at the end phases of sausage production. In each collection, samples were taken from the carcasses of eight sows through 11 stages of sausage manufacturing. A total of 830 samples were cultured. Thirty-four Salmonella were isolated from the final three production stages; of these, there were eleven serotypes. Three isolates displayed resistance to ampicillin, whereas the remainder of the isolates were pan-susceptible to the antimicrobials tested. Salmonella and EB were significantly reduced (p < 0.001) by acid washes at different stages of production, and the results point to the beneficial effects of interventions to lessen Salmonella concentrations in retail products.
Sexually Transmitted Infections in Italian Young and Adult People: A Worrying Positive Trend Hidden by COVID-19 Epidemic
Authors: Zanotta Nunzia , Magni Elena , De Seta Francesco , Petix Vincenzo , Sossi Karin , Colli Claudia , Monasta Lorenzo , Suligoi Barbara , Comar Manola
Published: August 2024

Recent European data show an increase in sexually transmitted infections (STIs), particularly N. gonorrhoeae, among young heterosexuals, surpassing pre-pandemic numbers. Italy’s varied local health restrictions during the COVID-19 pandemic likely affected STI management and reporting. To evaluate COVID-19’s impact on STI spread in Italy, we analyzed microbiological data from before and during the pandemic in an area with minimal restrictions on clinical services. This retrospective study (2017–2022) included 5503 subjects: 2586 from STI clinics (STD group) and 3687 diagnosed with primary infertility (ART group). Samples were tested for Mycoplasmas/Ureaplasmas, C. trachomatis, N. gonorrhoeae, and T. vaginalis by a multiplex PCR. During the pandemic, overall STI prevalence increased significantly (p < 0.01). U. parvum was the most frequent microorganism in the STD group (26.1% vs. 23.9%), with a notable increase in women (52.1% vs. 32.7%) (p < 0.001). C. trachomatis and M. hominis positive rates decreased significantly (p < 0.001 and p < 0.01, respectively). N. gonorrhoeae cases rose among young people (19–29), predominantly heterosexual, with high ciprofloxacin resistance. In the ART group, U. parvum was the most common infection, particularly in young infertile women (p = 0.01). This study indicates a notable rise in STIs among young people, including heterosexuals, despite social restrictions. The long-term impact of this trend requires further evaluation.
A Pilot Study for the Characterization of Bacillus spp. and Analysis of Possible B. thuringiensis/Strongyloides stercoralis Correlation
Authors: Pomari Elena , Orza Pierantonio , Bernardi Milena , Fracchetti Fabio , Campedelli Ilenia , De Marta Patrick , Recchia Alessandra , Paradies Paola , Buonfrate Dora
Published: August 2024

Differentiating between Bacillus species is relevant in human medicine. Bacillus thuringiensis toxins might be effective against Strongyloides stercoralis, a nematode causing relevant human morbidity. Our first objective was to evaluate genomic and MALDI-TOF identification methods for B. thuringiensis. Our secondary objective was to evaluate a possible negative selection pressure of B. thuringiensis against S. stercoralis. PCR and Sanger were compared to MALDI-TOF on a collection of 44 B. cereus group strains. B. thuringiensis toxin genes were searched on 17 stool samples from S. stercoralis-infected and uninfected dogs. Metagenomic 16S rRNA was used for microbiome composition. The inter-rate agreement between PCR, Sanger, and MALDI-TOF was 0.631 k (p-value = 6.4 × 10−10). B. thuringiensis toxins were not found in dogs’ stool. Bacteroidota and Bacillota were the major phyla in the dogs’ microbiome (both represented >20% of the total bacterial community). Prevotella was underrepresented in all Strongyloides-positive dogs. However, the general composition of bacterial communities was not significantly linked with S. stercoralis infection. The genomic methods allowed accurate differentiation between B. thuringiensis and B. cereus. There was no association between B. thuringiensis and S. stercoralis infection, but further studies are needed to confirm this finding. We provide the first descriptive results about bacterial fecal composition in dogs with S. stercoralis infection.
The New Genus Caulinema Revealed New Insights into the Generic Relationship of the Order Ulotrichales (Ulvophyceae, Chlorophyta)
Authors: Darienko Tatyana , Rad-Menéndez Cecilia , Pröschold Thomas
Published: August 2024

Traditionally, the order Ulotrichales comprised green algae of an unbranched, uniseriate, filamentous morphology. However, since the establishment of ultrastructural features, the circumscription of this order has dramatically changed. Some genera and species have been excluded from this order and others with different morphologies (sarcinoid, branched filaments or even parenchymatous taxa) have been included. Phylogenetic analyses have confirmed the monophyly of this order, but its differentiation from the Ulvales and Acrosiphoniales remains difficult because of the lack of synapomorphies at every level (morphology, molecular signatures). To demonstrate the difficulties of placement into genera and orders, we investigated two sarcinoid taxa with the absence of zoospore formation. SSU and ITS rDNA tree topology and the ITS-2/CBC approach revealed that both strains SAG 2661 and CCAP 312/1 belong to Ulosarcina terrestrica and the newly erected genus Caulinema, respectively. The species conception using this approach was evaluated by sequencing the plastid-coding gene tufA, a commonly used barcode marker for green algae. All three molecular markers resulted in similar topologies at the generic and species levels, which is consistent with the ITS-2/CBC approach and tufA for barcoding. The reevaluation of the ultrastructural features revealed that the presence of organic scales on the surfaces of motile cells is characteristic for the order Ulotrichales and can be used for separation from the closely related orders. As a consequence of our study, we propose the new genus Caulinema for strain CCAP 312/1.
Antimicrobial Effect of Chitosan Nanoparticles and Allium Species on Mycobacterium tuberculosis and Several Other Microorganisms
Authors: Olivas-Flores Jocelyn , Chávez-Méndez José Román , Castillo-Martínez Nydia Alejandra , Sánchez-Pérez Héctor Javier , Serrano-Medina Aracely , Cornejo-Bravo José Manuel
Published: August 2024

This study evaluates the antimicrobial efficacy of chitosan nanoparticles (CNPs), varying in size, against clinical isolates of Mycobacterium tuberculosis (MTB), E. coli, S. aureus, E. faecalis, and C. albicans, as well as the antimicrobial effects of aqueous extracts and lyophilized powders of Allium (garlic) species. CNPs were synthesized through ionotropic gelation and characterized by Z potential, hydrodynamic diameter (dynamic light scattering, DLS), and SEM. Aqueous garlic extracts were prepared via decoction. We assessed antimicrobial activity using disk diffusion and broth microdilution methods; in addition, a modified agar proportion method in blood agar was used for antimicrobial activity against MTB. CNPs inhibited MTB growth at 300 μg for 116.6 nm particles and 400 μg for 364.4 nm particles. The highest antimicrobial activity was observed against E. faecalis with nanoparticles between 200 and 280 nm. Allium sativum extract produced inhibition for C. albicans at 100 μg. The results indicate that CNPs possess significant antimicrobial properties against a range of pathogens, including MTB, at high concentrations. On the other hand, aqueous Allium sativum extracts exhibited antimicrobial activity. Nonetheless, due to their instability in solution, the use of lyophilized Allium sativum powder is preferable.
Elevating Skincare Science: Grape Seed Extract Encapsulation for Dermatological Care
Authors: Castro Maria Leonor , Azevedo-Silva João , Valente Diana , Machado Adriana , Ribeiro Tânia , Ferreira João Paulo , Pintado Manuela , Ramos Oscar L. , Borges Sandra , Baptista-Silva Sara
Published: August 2024

The skin is the largest organ in the human body and serves multiple functions such as barrier protection and thermoregulation. The maintenance of its integrity and healthy structure is of paramount importance. Accordingly, technological advances in cosmetic sciences have been directed towards optimizing these factors. Plant-derived ingredients have been explored for their bioactivity profiles and sustainable sources. Grape by-products contain a group of bioactive molecules that display important biological activities. Nonetheless, many of these molecules (e.g., phenolic compounds) are unstable and susceptible to degradation. So, their encapsulation using nano/microsystems (i.e., microdispersions) has been explored as a promising solution. In this work, two grape seed extracts were obtained, one from a single grape variety (GSE-Ov) and another from a mix of five grape varieties (GSE-Sv). These extracts were analysed for their antioxidant and antimicrobial activities, as well as their chemical composition and molecular structure. The extract that showed the most promising properties was GSE-Ov with a DPPH IC50 of 0.079 mg mL−1. This extract was encapsulated in soy lecithin microdispersions coated with pectin, with an encapsulation efficiency of 88.8%. They showed an in vitro release of polyphenols of 59.4% during 24 h. The particles displayed a zeta potential of −20.3 mV and an average diameter of 13.6 µm. Microdispersions proved to be safe under 5 and 2.5 mg mL−1 in HaCaT and HDF cell models, respectively. Additionally, they demonstrated anti-inflammatory activity against IL-1α when tested at 2 mg mL−1. This work enabled the valorisation of a by-product from the wine industry by using natural extracts in skincare products.
Synthesis, X-ray Crystallography, Spectroscopic Characterizations, Density Functional Theory, and Hirshfeld Surface Analyses of a Novel (Carbonato) Picket Fence Iron(III) Complex
Authors: Dhifet Mondher , Gassoumi Bouzid , Lutoshkin Maxim A. , Kazachenko Anna S. , Kazachenko Aleksandr S. , Al-Dossary Omar , Issaoui Noureddine , Nasri Habib
Published: August 2024

An Fe(III)-carbonato six-coordinate picket fence porphyrin complex with the formula [K(2,2,2-crypt)][FeIII(TpivPP)(CO3)]·C6H5Cl·3H2O (I) has been synthesized and characterized by UV-Vis and FT-IR spectra. The structure of (carbonato)(α,α,α,α-tetrakis(o-pivalamidophenyl)porphinato)ferrate(III) was also established by XRD. The iron atom is hexa-coordinated by the four nitrogen atoms of the pyrrol rings and the two oxygen atoms of the CO32− group. Complex I, characterized as a ferric high-spin complex (S = 5/2), presented higher Fe-Np (2.105(6) Å) and Fe-PC (0.654(2) Å) distances. Both X-ray molecular structure and Hirshfeld surface analysis results show that the crystal packing of I is made by C-H⋯O and C-H⋯Cg weak intermolecular hydrogen interactions involving neighboring [FeIII(TpivPP)(CO3)]− ion complexes. Computational studies were carried out at DFT/B3LYP-D3/LanL2DZ to investigate the HOMO and LUMO molecular frontier orbitals and the reactivity within the studied compound. The stability of compound I was investigated by analyzing both intra- and inter-molecular interactions using the 2D and 3DHirshfeld surface (HS) analyses. Additionally, the frontier molecular orbital (FMO) calculations and the molecular electronic potential (MEP) analyses were conducted to determine the electron localizations, electrophilic, and nucleophilic regions, as well as charge transfer (ECT) within the studied system.
Modification of the Protein Amino Acid Content in Hen Eggs as a Consequence of Different Concentrations of Lupine and Soy in Feed
Authors: Tomczak Aneta , Zielińska-Dawidziak Magdalena , Klimowicz Piotr , Hejdysz Marcin , Kaczmarek Sebastian , Siger Aleksander , Cieślak Adam
Published: August 2024

The effect of the diet modification (soybean and lupine addition) on the content of protein and amino acids (AA) in eggs was studied. Both the sampling day and the diet influenced the total protein content. In albumen, the lowest protein content (10.6%) was noted after administering a diet containing 25% lupine; in the same egg the yolk contained the most proteins (16.7%). In the content of nonessential AA (NAA) in egg yolks, differences were noted only for cysteine, with its the highest content in the yolks of the control group. The stable content of essential yolk amino acids (EAA) was observed only for isoleucine, leucine, tryptophan and phenylalanine. The highest contents of EAA and NAA were recorded in the yolks of the control group (~47 and ~53 g/100 g of protein, respectively) and in the group with 25% additions of lupine (~42 and ~51 g/100 g of protein, respectively). AA with constant content in the tested albumens were methionine, tryptophan and alanine. The highest content of EAA (>~42 g/100 g of protein) and NAA (>~62 g/100 g of protein) were determined in albumen of eggs determined in the group with at least 20% additions of lupine. The highest content of EAA for humans delivered eggs from groups 4–6 (with the addition of soy into the diet ≤5%). The protein sources used in the hen diet significantly influenced the content of protein and individual AA in the produced eggs.
New Insights into ZIF-90 Synthesis
Authors: Marčec Jan , Ristić Alenka , Logar Nataša Zabukovec
Published: August 2024

Zeolitic imidazolate frameworks (ZIFs) are traditionally synthesized using N, N-dimethylformamide (DMF). However, DMF is toxic and hazardous to human health and the environment, hence other alternative solvents need to be considered. Herein, three different solvents like methanol, water and acetone were used to replace DMF and to explore the syntheses of ZIF-90 using a conventional and a microwave-assisted solvothermal method to obtain hydrothermally stable products, which also exhibit an increased water uptake. Pure ZIF-90 was synthesized under ambient pressure at 60 °C for 90 min using the conventional solvothermal method in an acetone–water solution, while under microwave irradiation it was formed in only 5 min at 80 °C. Altering methanol, water and acetone in the reaction mixture significantly affected the structural and water adsorption properties of ZIF-90s, which were monitored via PXRD, TGA, nitrogen and water sorption, and SEM. The highly efficient, less toxic, low-cost and activation-free microwave synthesis resulted in the formation of ZIF-90 nanoparticles that exhibited the highest maximum water adsorption capacity (0.37 g/g) and the best hydrothermal stability between water adsorption at 30 °C and desorption at 100 °C at 12.5 mbar among all the products obtained.