bluuebunny/crossref_metadata_2025 huggingface dataset:
Healing of Recurrent Aphthous Stomatitis by Non-Thermal Plasma: Pilot Study
Authors: Ibáñez-Mancera Norma Guadalupe , López-Callejas Régulo , Toral-Rizo Víctor Hugo , Rodríguez-Méndez Benjamín Gonzalo , Lara-Carrillo Edith , Peña-Eguiluz Rosendo , do Amaral Regiane Cristina , Mercado-Cabrera Antonio , Valencia-Alvarado Raúl
Published: January 2023

Recurrent aphthous stomatitis (RAS) is a common disease in the oral cavity characterized by recurrent ulcers (RU). Usually, these cause acute pain without definitive treatment. The present study determines the efficacy of non-thermal plasma (NTP) for treating RU. NTP is applied to the patient’s RU using a radiofrequency generator connected to a point reactor. The power density applied to the ulcer is 0.50 W/cm2, less than 4 W/cm2, which is the maximum value without biological risk. Each patient received two treatments of three minutes each and spaced 60 min apart at a distance of 5 mm from the RU. From a sample of 30 ulcers in patients treated for RU with an average age of 37 years, they stated that the pain decreased considerably and without the need for ingestion of analgesics and antibiotics. Regeneration took place in an average of three days. The NTP proved to be an excellent therapeutic alternative for the treatment of RU since it has a rapid effect of reducing pain and inflammation, as well as adequate tissue regeneration.
Laser-Induced Graphene Arrays-Based Three-Phase Interface Enzyme Electrode for Reliable Bioassays
Authors: Zhang Man , Zhang Jun , Ding Zhenyao , Wang Haili , Huang Lihui , Feng Xinjian
Published: January 2023

Electrochemical oxidase biosensors have been widely applied in healthcare, environmental measurements and the biomedical field. However, the low and fluctuant oxygen levels in solution and the high anodic detection potentially restrict the assay accuracy. To address these problems, in this work, we constructed a three-phase interface enzyme electrode by sequentially immobilizing H2O2 electrocatalysts and an oxidase layer on a superhydrophobic laser-induced graphene (LIG) array substrate. The LIG-based enzyme electrode possesses a solid–liquid–air three-phase interface where constant and sufficient oxygen can be supplied from the air phase to the enzymatic reaction zone, which enhances and stabilizes the oxidase kinetics. We discovered that the enzymatic reaction rate is 21.2-fold improved over that of a solid–liquid diphase system where oxygen is supplied from the liquid phase, leading to a 60-times wider linear detection range. Moreover, the three-phase enzyme electrode can employ a cathodic measuring principle for oxidase catalytic product H2O2 detection, which could minimize interferences arising from oxidizable molecules in biofluids and increase the detection selectivity. This work provides a simple and promising approach to the design and construction of high-performance bioassay systems.
A Fiber Bragg Grating Sensor Based on Cladding Mode Resonance for Label-Free Biosensing
Authors: Chen Shimeng , Zhang Chao , Wang Jiahui , Li Na , Song Yongxin , Wu Haojun , Liu Yun
Published: January 2023

A fiber-optic biosensing platform based on ultra-narrowband cladding mode resonances was developed on a high-reflectivity fiber Bragg grating (FBG) for targeting biomolecular detection. The multiple cladding modes with a high sensitivity to the refractive index (RI) were excited in the FBG by coupling between the forward-propagating guided core mode of the multimode fiber and the backward-propagating guided cladding mode of the FBG without any damage to the fiber structure or any change to the standard FBG manufacturing process. The full width at half maximum and the Q-factor of the typical cladding mode resonance operation of the proposed sensor are 80 pm and 19,270, respectively, which are better than those of most fiber-optic biosensors reported to date. In addition, the FBG sensor demonstrated a high sensitivity in protein detection and a high selectivity in serum sample assays. The sensitivity of this sensor was further increased simply by coating it with graphene oxide (GO) sheets on the sensing surface without using a signal amplification strategy. Furthermore, an ultra-low limit of detection (LOD) of 32 pM was obtained by the GO-coated FBG sensor for IgG detection. The proposed FBG sensor provides a competitive fiber-optic platform for biomolecular detection. It has a great potential for applications in label-free biosensing.
Recent Progress in Nanomaterial-Based Biosensors and Theranostic Nanomedicine for Bladder Cancer
Authors: Song Fan-Xin , Xu Xiaojian , Ding Hengze , Yu Le , Huang Haochen , Hao Jinting , Wu Chenghao , Liang Rui , Zhang Shaohua
Published: January 2023

Bladder cancer (BCa) is one of the most expensive and common malignancies in the urinary system due to its high progression and recurrence rate. Although there are various methods, including cystoscopy, biopsy, and cytology, that have become the standard diagnosis methods for BCa, their intrinsic invasive and inaccurate properties need to be overcome. The novel urine cancer biomarkers are assisted by nanomaterials-based biosensors, such as field-effect transistors (FETs) with high sensitivity and specificity, which may provide solutions to these problems. In addition, nanomaterials can be applied for the advancement of next-generation optical imaging techniques and the contrast agents of conventional techniques; for example, magnetic resonance imaging (MRI) for the diagnosis of BCa. Regarding BCa therapy, nanocarriers, including mucoadhesive nanoparticles and other polymeric nanoparticles, successfully overcome the disadvantages of conventional intravesical instillation and improve the efficacy and safety of intravesical chemotherapy for BCa. Aside from chemotherapy, nanomedicine-based novel therapies, including photodynamic therapy (PDT), photothermal therapy (PTT), chemodynamic therapy (CDT), sonodynamic therapy (SDT), and combination therapy, have afforded us new ways to provide BC therapy and hope, which can be translated into the clinic. In addition, nanomotors and the nanomaterials-based solid tumor disassociation strategy provide new ideas for future research. Here, the advances in BCa diagnosis and therapy mentioned above are reviewed in this paper.
Elevated Plasma Oligomeric Amyloid β-42 Is Associated with Cognitive Impairments in Cerebral Small Vessel Disease
Authors: Qu Wensheng , Zhang Liding , Liang Xiaohan , Yu Zhiyuan , Huang Hao , Zhao Jing , Guo Yinping , Zhou Xirui , Xu Shabei , Luo Haiming , Luo Xiang
Published: January 2023

Due to the heterogeneity of amyloid β-42 (Aβ42) species, the potential correlation between plasma oligomeric Aβ42 (oAβ42) and cognitive impairments in cerebral small vessel disease (CSVD) remains unclear. Herein, a sandwich ELISA for the specific detection of Aβ42 oligomers (oAβ42) and total Aβ42 (tAβ42) was developed based on sequence- and conformation-specific antibody pairs for the evaluation of plasma samples from a Chinese CSVD community cohort. After age and gender matching, 3-Tesla magnetic resonance imaging and multidimensional cognitive assessment were conducted in 134 CSVD patients and equal controls. The results showed that plasma tAβ42 and oAβ42 levels were significantly elevated in CSVD patients. By regression analysis, these elevations were correlated with the presence of CSVD and its imaging markers (i.e., white matter hyperintensities). Plasma Aβ42 tests further strengthened the predictive power of vascular risk factors for the presence of CSVD. Relative to tAβ42, oAβ42 showed a closer correlation with memory domains evaluated by neuropsychological tests. In conclusion, this sensitive ELISA protocol facilitated the detection of plasma Aβ42; Aβ42, especially its oligomeric form, can serve as a biosensor for the presence of CSVD and associated cognitive impairments represented by memory domains.
An Effective Electrochemical Platform for Chloramphenicol Detection Based on Carbon-Doped Boron Nitride Nanosheets
Authors: Yin Jingli , Ouyang Huiying , Li Weifeng , Long Yumei
Published: January 2023

Currently, accurate quantification of antibiotics is a prerequisite for health care and environmental governance. The present work demonstrated a novel and effective electrochemical strategy for chloramphenicol (CAP) detection using carbon-doped hexagonal boron nitride (C-BN) as the sensing medium. The C-BN nanosheets were synthesized by a molten-salt method and fully characterized using various techniques. The electrochemical performances of C-BN nanosheets were studied using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The results showed that the electrocatalytic activity of h-BN was significantly enhanced by carbon doping. Carbon doping can provide abundant active sites and improve electrical conductivity. Therefore, a C-BN-modified glassy carbon electrode (C-BN/GCE) was employed to determine CAP by differential pulse voltammetry (DPV). The sensor showed convincing analytical performance, such as a wide concentration range (0.1 µM–200 µM, 200 µM–700 µM) and low limit of detection (LOD, 0.035 µM). In addition, the proposed method had high selectivity and desired stability, and can be applied for CAP detection in actual samples. It is believed that defect-engineered h-BN nanomaterials possess a wide range of applications in electrochemical sensors.
Novel CaLB-like Lipase Found Using ProspectBIO, a Software for Genome-Based Bioprospection
Authors: Brêda Gabriela C. , Faria Priscila E. , Rodrigues Yuri S. , Pinheiro Priscila B. , Nucci Maria Clara R. , Ferrer Pau , Freire Denise M. G. , Almeida Rodrigo V. , Mesquita Rafael D.
Published: January 2023

Enzymes have been highly demanded in diverse applications such as in the food, pharmaceutical, and industrial fuel sectors. Thus, in silico bioprospecting emerges as an efficient strategy for discovering new enzyme candidates. A new program called ProspectBIO was developed for this purpose as it can find non-annotated sequences by searching for homologs of a model enzyme directly in genomes. Here we describe the ProspectBIO software methodology and the experimental validation by prospecting for novel lipases by sequence homology to Candida antarctica lipase B (CaLB) and conserved motifs. As expected, we observed that the new bioprospecting software could find more sequences (1672) than a conventional similarity-based search in a protein database (733). Additionally, the absence of patent protection was introduced as a criterion resulting in the final selection of a putative lipase-encoding gene from Ustilago hordei (UhL). Expression of UhL in Pichia pastoris resulted in the production of an enzyme with activity towards a tributyrin substrate. The recombinant enzyme activity levels were 4-fold improved when lowering the temperature and increasing methanol concentrations during the induction phase in shake-flask cultures. Protein sequence alignment and structural modeling showed that the recombinant enzyme has high similarity and capability of adjustment to the structure of CaLB. However, amino acid substitutions identified in the active pocket entrance may be responsible for the differences in the substrate specificities of the two enzymes. Thus, the ProspectBIO software allowed the finding of a new promising lipase for biotechnological application without the need for laborious and expensive conventional bioprospecting experimental steps.
Frontal Encephalocele Plus Epilepsy: A Case Report and Review of the Literature
Authors: Yamazaki Ken , Kanaya Kohei , Uda Takehiro , Fukuyama Tetsuhiro , Nishioka Makoto , Hoshino Yumi , Kaneko Tomoki , Hardian Ridzky Firmansyah , Yamazaki Daisuke , Kuwabara Haruki , Funato Kohei , Horiuchi Tetsuyoshi
Published: January 2023

An encephalocele is a pathological brain herniation caused by osseous dural defects. Encephaloceles are known to be regions of epileptogenic foci. We describe the case of a 44-year-old woman with refractory epilepsy associated with a frontal skull base encephalocele. Epilepsy surgery for encephalocele resection was performed; however, the epilepsy was refractory. A second epilepsy surgery for frontal lobectomy using intraoperative electroencephalography was required to achieve adequate seizure control. Previous reports have shown that only encephalocele resection can result in good seizure control, and refractory epilepsy due to frontal lobe encephalocele has rarely been reported. To the best of our knowledge, this is the first report of frontal encephalocele plus epilepsy in which good seizure control using only encephalocele resection was difficult to achieve. Herein, we describe the possible mechanisms of encephalocele plus epilepsy and the surgical strategy for refractory epilepsy with encephalocele, including a literature review.
Influence of the Vertical Component of Yangbi Ground Motion on the Dynamic Response of RC Frame and Brick-Concrete Structure
Authors: Wang Hongwei , Jia Mingming , Yao Yanwu , Chen Xueliang , Zhang Zirong
Published: January 2023

An earthquake of magnitude 6.4 occurred in Yangbi County, Yunnan Province on 21 May 2021, with a focal depth of 8 km, and strong ground motion with vertical components was monitored by Yangbi station (53YBX). A total of 14,122 houses were damaged in Yangbi in the earthquake, and 232 of them collapsed. Vertical components of ground motions have been gained more attention for its effect on structure’s seismic response in epicenter or near-fault regions at present. Taking the three earthquake ground motions of Yangbi, Chi-Chi, and Loma Prieta as inputs, and modeling based on Perform-3D, this research carried out the seismic dynamic time history analysis of an RC (reinforced concrete) frame structure and a brick-concrete structure under both horizontal and vertical working conditions. The results showed that vertical components of the three ground motions had no evident impact on the top horizontal displacement and acceleration of the two types of structures. Among the three ground motions, the vertical component of Yangbi ground motion has largely influenced the top vertical displacement, acceleration, and axial force of the frame column bottom (or masonry wall bottom). The vertical component had different amplification effects on the axial pressure and the bending moment of a single column at the bottom of the RC frame structure, thus causing resonance amplification effect of the brick-concrete structure floors and amplifying the vertical acceleration of the top floor. In addition, it considerably increase the maximum axial tensile strain of masonry walls and the possibility of faster tensile failure of the brick-concrete structure. Influence of vertical ground motion on the bearing capacity of RC frame structure’s columns and the brick-concrete structure’s masonry walls should not be ignored. The results of the research may provide a reference for the earthquake-resistant design of building structures, especially the earthquake-resistant design considering the vertical seismic effect.
Optimal Site Selection for Women University Using Neutrosophic Multi-Criteria Decision Making Approach
Authors: Alzahrani Fahad A. , Ghorui Neha , Gazi Kamal Hossain , Giri Bibhas Chandra , Ghosh Arijit , Mondal Sankar Prasad
Published: January 2023

Site selection for an institute or a university is a challenging task. The selection of sites for setting up a new university depends on multiple criteria. In backward, under privileged area people’s perception towards the co-educational universities and women universities are different. Poor families with their conservative mentality possess inhibitions while sending their girl child to co-educational universities as they have concerns about safety, security and family honor. Hence many attributes which are not so important for co-educational universities are more pertinent for women university. In this research paper, we have considered a model for selecting women’s university sites in different backward locations in the state of West Bengal, India. This model incorporated different types of uncertainty related to site selection. Ten important criteria are chosen for the selection of sites. To capture the uncertainty of the problem, trapezoidal neutrosophic numbers are used along with the Multi-criteria Decision Making tool Analytic Hierarchy Process (AHP) for obtaining criteria weights. Finally, the Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) and COmplex PRoportional ASsessment (COPRAS) are applied for ranking of the sites. Comparative and sensitivity analyses are conducted to check the steadiness of the techniques used.
Historical and Physicochemical Analysis of the Clinker Bricks in the Smart Memorial Gymnasium of the Tiancizhuang Campus at Soochow University, China
Authors: Wang Shiruo , Gao Jiao , Wang Xiaomu , Wu Dan , Pan Yiting , Xu Minmin
Published: January 2023

Clinker bricks were popular as a facade material in the United States between the 1890s and the 1930s. However, this material was unknown to Chinese builders and was seldom found in Chinese modern architecture from 1840 to 1949. The Smart Memorial Gymnasium built in the years 1934–1937 in the Tiancizhuang Campus of Soochow University (Suzhou, China) is one of the rare examples of a building featuring clinker bricks in modern China. Notably, those clinker bricks were not imported but locally manufactured. Despite the heritage significance of the Smart Memorial Gymnasium as part of a major historical and cultural site protected at the national level in China, the history and characteristics of those historical bricks have remained virtually unexplored. This study first provides a historical analysis of those bricks, giving insights into the general knowledge of this construction material around that time based on British and American historical sources from the 19th and 20th centuries, with a focus on historical treaties and documents. This analysis sheds light on the raw materials mixtures of clinker bricks, their manufacturing processes, and their architectural applications at the time. Moreover, this study presents a physicochemical analysis of the clinker bricks employed at Soochow University, focusing on the correlation between historical studies and physicochemical characteristics, as well as the materials’ characteristics that respond to the natural environment. X-ray diffraction (XRD), Raman spectroscopy, scanning electron microscopy with energy dispersive spectroscopy (SEM-EDS), and total immersion tests were employed to investigate the physicochemical properties of the bricks at various locations of the Smart Memorial Gymnasium facades. Our findings deepen the knowledge and understanding of clinker bricks transferred from the West to China in the early 20th century. Additionally, our results reveal the chemical composition and physical characteristics of different types of clinker bricks used in the Smart Memorial Gymnasium, outlining practical implications and future research directions. Overall, this study lays a foundation for the heritage recognition and conservation of Chinese clinker bricks.
Digital Technologies in Offsite and Prefabricated Construction: Theories and Applications
Authors: Cheng Zhuo , Tang Shengxian , Liu Hexu , Lei Zhen
Published: January 2023

Due to its similarity to industrialized products, the offsite construction industry is seen as a focus for the transformation of Construction 4.0. Many digital technologies have been applied or have the potential to be applied to realize the integration of design, manufacturing, and assembly. The main objective of this review was to identify the current stage of applying digital technologies in offsite construction. In this review, 171 related papers from the last 10 years (i.e., 2013–2022) were obtained by collecting and filtering them. They were classified and analyzed according to the digital twin concept, application areas, and specific application directions. The results indicated that there are apparent differences in the utilization and development level of different technologies in different years. Meanwhile, the introduction, current stages, and benefits of different digital technologies are also discussed. Finally, this review summarizes the current popular fields and speculates on future research directions by analyzing article publication trends, which sheds light on future research.
Regression Models for Predicting the Global Warming Potential of Thermal Insulation Materials
Authors: Tajuddeen Ibrahim , Sajjadian Seyed Masoud , Jafari Mina
Published: January 2023

The impacts and benefits of thermal insulations on saving operational energy have been widely investigated and well-documented. Recently, many studies have shifted their focus to comparing the environmental impacts and CO2 emission-related policies of these materials, which are mostly the Embodied Energy (EE) and Global Warming Potential (GWP). In this paper, machine learning techniques were used to analyse the untapped aspect of these environmental impacts. A collection of over 120 datasets from reliable open-source databases including Okobaudat and Ecoinvent, as well as from the scientific literature containing data from the Environmental Product Declarations (EPD), was compiled and analysed. Comparisons of Multiple Linear Regression (MLR), Support Vector Regression (SVR), Least Absolute Shrinkage and Selection Operator (LASSO) regression, and Extreme Gradient Boosting (XGBoost) regression methods were completed for the prediction task. The experimental results revealed that MLR, SVR, and LASSO methods outperformed the XGBoost method according to both the K-Fold and Monte-Carlo cross-validation techniques. MLR, SVR, and LASSO achieved 0.85/0.73, 0.82/0.72, and 0.85/0.71 scores according to the R2 measure for the Monte-Carlo/K-Fold cross-validations, respectively, and the XGBoost overfitted the training set, showing it to be less reliable for this task. Overall, the results of this task will contribute to the selection of effective yet low-energy-intensive thermal insulation, thus mitigating environmental impacts.
Physico-Antibacterial Feature and SEM Morphology of Bio-Hydraulic Lime Mortars Incorporating Nano-Graphene Oxide and Binary Combination of Nano-Graphene Oxide with Nano Silver, Fly Ash, Zinc, and Titanium Powders
Authors: Kırgız Mehmet Serkan , Mirza Jahangir , Cuc Stanca , Prodan Doina , Saroşi Codruţa , Perhaiţă Ioana , Carpa Rahela , Moldovan Marioara , Popa Dorin , Varvara Simona , Popa Maria
Published: January 2023

The study evaluated the impact of graphene powders used as additives in the recipe of the experimental lime mortar to a mixture ratio of 1:2.5 of NHL3.5 hydraulic lime:fine sand. The content of binder, aggregate and water was kept constant, varying only the amount and the type of the added additives in relation to the amount of natural hydraulic lime NHL3.5. The following five types of experimental mortars were prepared as follows: reference mortar (without additive); mortars containing 1 wt.% GO and 5 wt.% GO powder; mortar with the following GO powders mixture: GO powder functionalized with silver nanoparticles and with fly ash (GO-Ag + GO-fly ash); mortar with the following GO powders mixture: GO with zinc oxide and with titanium oxide (GO-ZnO + GO-TiO2). The influence of the GO-based additive addition on the porosity, surface microstructure, and water sorption coefficient of the mortar samples was evaluated. The antibacterial effect of the mortar samples against three bacterial strains was also investigated. The best results were obtained for the experimental mortar containing GO-ZnO -TiO2, which showed improved experimental properties that potentially allow its use for the rehabilitation of heritage buildings.
Oncology-Led Early Identification of Nutritional Risk: A Pragmatic, Evidence-Based Protocol (PRONTO)
Authors: Muscaritoli Maurizio , Bar-Sela Gil , Battisti Nicolo Matteo Luca , Belev Borislav , Contreras-Martínez Jorge , Cortesi Enrico , de Brito-Ashurst Ione , Prado Carla M. , Ravasco Paula , Yalcin Suayib
Published: January 2023

Nutritional issues, including malnutrition, low muscle mass, sarcopenia (i.e., low muscle mass and strength), and cachexia (i.e., weight loss characterized by a continuous decline in skeletal muscle mass, with or without fat loss), are commonly experienced by patients with cancer at all stages of disease. Cancer cachexia may be associated with poor nutritional status and can compromise a patient’s ability to tolerate antineoplastic therapy, increase the likelihood of post-surgical complications, and impact long-term outcomes including survival, quality of life, and function. One of the primary nutritional problems these patients experience is malnutrition, of which muscle depletion represents a clinically relevant feature. There have been recent calls for nutritional screening, assessment, treatment, and monitoring as a consistent component of care for all patients diagnosed with cancer. To achieve this, there is a need for a standardized approach to enable oncologists to identify patients commencing and undergoing antineoplastic therapy who are or who may be at risk of malnutrition and/or muscle depletion. This approach should not replace existing tools used in the dietitian’s role, but rather give the oncologist a simple nutritional protocol for optimization of the patient care pathway where this is needed. Given the considerable time constraints in day-to-day oncology practice, any such approach must be simple and quick to implement so that oncologists can flag individual patients for further evaluation and follow-up with appropriate members of the multidisciplinary care team. To enable the rapid and routine identification of patients with or at risk of malnutrition and/or muscle depletion, an expert panel of nutrition specialists and practicing oncologists developed the PROtocol for NuTritional risk in Oncology (PRONTO). The protocol enables the rapid identification of patients with or at risk of malnutrition and/or muscle depletion and provides guidance on next steps. The protocol is adaptable to multiple settings and countries, which makes implementation feasible by oncologists and may optimize patient outcomes. We advise the use of this protocol in countries/clinical scenarios where a specialized approach to nutrition assessment and care is not available.
Palmitic Acid Inhibits the Growth and Metastasis of Gastric Cancer by Blocking the STAT3 Signaling Pathway
Authors: Yu Xiaojuan , Peng Wen , Wang Yaoxing , Xu Wenjun , Chen Wentong , Huang Lei , Xu Hu , He Xinyu , Wang Sheng , Sun Qianqian , Lu Wenjie , Xu Youzhi
Published: January 2023

Lipidomic analyses have suggested that palmitic acid (PA) is linked to gastric cancer. However, its effects and action mechanisms remain unclear. Therefore, we evaluated the effects of PA on cell proliferation, invasion, and apoptosis in human gastric cancer, as well as the role of p-STAT3 in mediating its effects. The results of the MTT and colony formation assays revealed that PA blocked gastric cancer cell proliferation in a concentration-dependent manner. The EdU-DNA assay indicated that 50 μM of PA could block gastric cell proliferation by 30.6–80.0%. The Transwell assay also confirmed the concentration dependence of PA-induced inhibitory effect on cell invasion. The flow cytometry analysis indicated that PA treatment for 18 h could induce gastric cancer cell apoptosis. The immunohistochemical staining revealed that p-STAT3 levels were higher in the gastric cancer tissues than in the control tissues. We demonstrated that PA treatment for 12 h decreased the expressions of p-STAT3, p-JAK2, N-cadherin, and vimentin, and inhibited the nuclear expression of p-STAT3 in gastric cancer cells. Finally, PA treatment (50 mg/kg) decreased gastric cancer growth (54.3%) in the xenograft models. Collectively, these findings demonstrate that PA inhibits cell proliferation and invasion and induces human gastric cancer cell apoptosis.
The Characteristics and Prognosis of Alpha-Fetoprotein and Des-Gamma-Carboxy Prothrombin Double-Negative Hepatocellular Carcinoma at Baseline in Higher BCLC Stages
Authors: Nagahara Takakazu , Sugihara Takaaki , Kihara Takuya , Ikeda Suguru , Hoshino Yoshiki , Matsuki Yukako , Sakaguchi Takuki , Kurumi Hiroki , Onoyama Takumi , Takata Tomoaki , Matono Tomomitsu , Yamaguchi Naoyuki , Isomoto Hajime
Published: January 2023

Alpha-fetoprotein (AFP) and des-gamma-carboxyprothrombin (DCP) are widely used as tumor markers to diagnose hepatocellular carcinoma (HCC). Some advanced HCCs demonstrate neither AFP nor DCP. This study investigated the characteristics and prognosis of AFP (<20 ng/mL) and DCP (<40 mAU/ml) double-negative HCC (DNHC) in higher-stage HCC. Between April 2012 and March 2022, 419 consecutive patients were enrolled with newly diagnosed HCC and 372 patients were selected that were diagnosed by histopathology and/or imaging. AFP-negative, DCP-negative, and double-negative HCC were identified in 262 patients (70.4%), 143 patients (38.2%), and 120 patients (32.3%), respectively. In higher-BCLC stages (BCLC-B, C, and D), 17 patients (14.7%) were DNHC. Although there was no difference in BCLC staging, there were more cases under TNM Stage III in DNHC (71.0% vs. 41.4%, p = 0.026). The median maximum tumor diameter was smaller in DNHC [3.2 (1.8–5.0) vs. 5.5 (3.5–9.0) cm, p = 0.001] and their median survival time was significantly better, even in higher-stage HCC [47.0 (24.0–84.0) vs. 19.0 (14.0–30.0) months, p = 0.027). DNHC in higher-BCLC stage HCC is independent of BCLC staging, characterized by a tumor diameter < 5 cm, and is treatable with a good prognosis.
Factors Associated with the Breast Cancer Diagnostic Interval across Five Canadian Provinces: A CanIMPACT Retrospective Cohort Study
Authors: Ruco Arlinda , Groome Patti A. , McBride Mary L. , Decker Kathleen M. , Grunfeld Eva , Jiang Li , Kendell Cynthia , Lofters Aisha , Urquhart Robin , Vu Khanh , Winget Marcy
Published: January 2023

The cancer diagnostic process can be protracted, and it is a time of great anxiety for patients. The objective of this study was to examine inter- and intra-provincial variation in diagnostic intervals and explore factors related to the variation. This was a multi-province retrospective cohort study using linked administrative health databases. All females with a diagnosis of histologically confirmed invasive breast cancer in British Columbia (2007–2010), Manitoba (2007–2011), Ontario (2007–2010), Nova Scotia (2007–2012), and Alberta (2004–2010) were included. The start of the diagnostic interval was determined using algorithms specific to whether the patient’s cancer was detected through screening. We used multivariable quantile regression analyses to assess the association between demographic, clinical and healthcare utilization factors with the diagnostic interval outcome. We found significant inter- and intra-provincial variation in the breast cancer diagnostic interval and by screen-detection status; patients who presented symptomatically had longer intervals than screen-detected patients. Interprovincial diagnostic interval variation was 17 and 16 days for screen- and symptom-detected patients, respectively, at the median, and 14 and 41 days, respectively, at the 90th percentile. There was an association of longer diagnostic intervals with increasing comorbid disease in all provinces in non-screen-detected patients but not screen-detected. Longer intervals were observed across most provinces in screen-detected patients living in rural areas. Having a regular primary care provider was not associated with a shorter diagnostic interval. Our results highlight important findings regarding the length of the breast cancer diagnostic interval, its variation within and across provinces, and its association with comorbid disease and rurality. We conclude that diagnostic processes can be context specific, and more attention should be paid to developing tailored processes so that equitable access to a timely diagnosis can be achieved.
Effects of Whole and Partial Heart Irradiation on Collagen, Mast Cells, and Toll-like Receptor 4 in the Mouse Heart
Authors: Sridharan Vijayalakshmi , Krager Kimberly J. , Pawar Snehalata A. , Bansal Shivani , Li Yaoxiang , Cheema Amrita K. , Boerma Marjan
Published: January 2023

In radiation therapy of tumors in the chest, such as in lung or esophageal cancer, part of the heart may be situated in the radiation field. This can lead to the development of radiation-induced heart disease. The mechanisms by which radiation causes long-term injury to the heart are not fully understood, but investigations in pre-clinical research models can contribute to mechanistic insights. Recent developments in X-ray technology have enabled partial heart irradiation in mouse models. In this study, adult male and female C57BL/6J mice were exposed to whole heart (a single dose of 8 or 16 Gy) and partial heart irradiation (16 Gy to 40% of the heart). Plasma samples were collected at 5 days and 2 weeks after the irradiation for metabolomics analysis, and the cardiac collagen deposition, mast cell numbers, and left ventricular expression of Toll-like receptor 4 (TLR4) were examined in the irradiated and unirradiated parts of the heart at 6 months after the irradiation. Small differences were found in the plasma metabolite profiles between the groups. However, the collagen deposition did not differ between the irradiated and unirradiated parts of the heart, and radiation did not upregulate the mast cell numbers in either part of the heart. Lastly, an increase in the expression of TLR4 was seen only after a single dose of 8 Gy to the whole heart. These results suggest that adverse tissue remodeling was not different between the irradiated and unirradiated portions of the mouse heart. While there were no clear differences between male and female animals, additional work in larger cohorts may be required to confirm this result, and to test the inhibition of TLR4 as an intervention strategy in radiation-induced heart disease.
Optimizing First-Line Chemotherapy in Metastatic Pancreatic Cancer: Efficacy of FOLFIRINOX versus Nab-Paclitaxel Plus Gemcitabine
Authors: Di Costanzo Francesco , Di Costanzo Federica , Antonuzzo Lorenzo , Mazza Ernesto , Giommoni Elisa
Published: January 2023

Pancreatic cancer (PC) is one of the most lethal tumors in Europe with an overall 5-year survival rate of 5%. Since 1992, gemcitabine (Gem) has been the treatment of choice for metastatic disease with significant improvement in median overall survival (OS) compared to fluorouracil. A good performance status (PS) at diagnosis appears to be a strong predictive factor for better survival. Overall, 50% of PC are metastatic or locally advanced at diagnosis, and more than 70% of the resected patients will experience a recurrence, with a median OS ranging from 4 to 10 months (mos). FOLFIRINOX (5-fluorouracil, leucovorin, irinotecan, and oxaliplatin) and Nab-paclitaxel (Nab-p) plus Gem have recently increased survival of patients with metastatic PC, over Gem. Treatment with FOLFIRINOX is generally considered more effective with respect to the doublet, with toxicity concerns, FOLFIRINOX achieves an overall response rate (ORR) of 31.6%, while for Nab-p plus Gem ORR is 23%; however, FOLFIRINOX was associated with higher rates of grade 3 and higher adverse events. Although the international guidelines indicate that both regimens can be used as first-line therapy for patients with metastatic PC, FOLFIRINOX is the most widely used; Nab-p plus Gem is more frequently used in patients with lower PS. In this review, we critically analyze these two regimens to give a pragmatic guide to treatment options.
Patient Reported Outcomes and Measures in Children with Rhabdomyosarcoma
Authors: van Gorp Marloes , Grootenhuis Martha A. , Darlington Anne-Sophie , Wakeling Sara , Jenney Meriel , Merks Johannes H. M. , Hjalgrim Lisa Lyngsie , Adams Madeleine
Published: January 2023

In addition to optimising survival of children with rhabdomyosarcoma (RMS), more attention is now focused on improving their quality of life (QOL) and reducing symptoms during treatment, palliative care or into long-term survivorship. QOL and ongoing symptoms related to the disease and its treatment are outcomes that should ideally be patient-reported (patient-reported outcomes, PROs) and can be assessed using patient-reported outcome measures (PROMS). This commentary aims to encourage PRO and PROM use in RMS by informing professionals in the field of available PROMs for utilisation in paediatric RMS and provide considerations for future use in research and clinical practice. Despite the importance of using PROMs in research and practice, PROMs have been reported scarcely in paediatric RMS literature so far. Available literature suggests lower QOL of children with RMS compared to general populations and occurrence of disease-specific symptoms, but a lack of an RMS-specific PROM. Ongoing developments in the field include the development of PROMs targeted at children with RMS specifically and expansion of PROM evaluation within clinical trials.
Copper-Based Metal–Organic Frameworks (MOFs) as an Emerging Catalytic Framework for Click Chemistry
Authors: Singh Riddima , Singh Gurleen , George Nancy , Singh Gurjaspreet , Gupta Sofia , Singh Harminder , Kaur Gurpreet , Singh Jandeep
Published: January 2023

In the extensive terrain of catalytic procedures for the synthesis of organic molecules, metal–organic frameworks (MOFs) as heterogenous catalysts have been investigated in a variety of chemical processes, including Friedel–Crafts reactions, condensation reactions, oxidations, and coupling reactions, and utilized owing to their specific properties such as high porosity, tuneability, extraordinary catalytic activity, and recyclability. The eminent copper-tailored MOF materials can be exceptionally dynamic and regioselective catalysts for click reactions (1,3-dipolar cycloaddition reaction). Considering the fact that Cu(I)-catalyzed alkyne–azide cycloaddition (CuAAC) reactions can be catalyzed by several other copper catalysts such as Cu (II)-β-cyclodextrin, Cu(OAc)2, Fe3O4@SiO2, picolinimidoamide–Cu(II) complex, and Cu(II) porphyrin graphene, the properties of sorption and reusability, as well as the high density of copper-MOFs, open an efficient and robust pathway for regimented catalysis of this reaction. This review provides a comprehensive description and analysis of the relevant literature on the utilization of Cu-MOFs as catalysts for CuAAC ‘click’ reactions published in the past decade.
Methanation of CO2 over High Surface Nickel/Aluminates Compounds Prepared by a Self-Generated Carbon Template
Authors: Roudane Sarra , Bettahar Noureddin , Caballero Alfonso , Holgado Juan Pedro
Published: January 2023

Catalytic gas-phase hydrogenation of CO2 into CH4 was tested under three different nickel/aluminate catalysts obtained from precursors of hexaaluminate composition (MAl16O19, M = Mg, Ca, Ba). These catalysts were prepared using a carbon template method, where carbon is self-generated from a sol-gel that contains an excess of citric acid and the Al and M salts (Ba2+, Ca2+, Mg2+) by two-step calcination in an inert/oxidizing atmosphere. This procedure yielded Ni particles decorating the surface of a porous high surface area matrix, which presents a typical XRD pattern of aluminate structure. Ni particles are obtained with a homogeneous distribution over the surface and an average diameter of ca 25–30 nm. Obtained materials exhibit a high conversion of CO2 below 500 °C, yielding CH4 as a final product with selectivity >95%. The observed trend with the alkaline earth cation follows the order NiBaAlO-PRx > NiCaAlO-PRx > NiMgAlO-PRx. We propose that the high performance of the NiBaAlO sample is derived from both an appropriate distribution of Ni particle size and the presence of BaCO3, acting as a CO2 buffer in the process.
Green Synthesis of Spirooxindoles via Lipase-Catalyzed One-Pot Tandem Reaction in Aqueous Media
Authors: Tang Yong , Wang Ciduo , Xie Hanqing , Xu Yuelin , Wang Chunyu , Du Chuang , Wang Zhi , Wang Lei
Published: January 2023

The development of non-natural enzymatic catalysis is important for multicomponent tandem organic transformations. However, the delicate acting environments of biological enzymes still present some challenges in the synthesis of spirooxindole skeleton via enzymatic catalysis. To address these issues, a lipase-catalyzed method was developed for the synthesis of spirooxindole frameworks. Using easily available isatins, cycloketones, and malononitriles as substrates, mild reaction conditions, and a reasonable reaction time, moderate to good yields (67–92%) and excellent functional group tolerance were accomplished via this protocol. The related mechanism explanation is also speculated in this paper.
Bienzymatic Cascade Combining a Peroxygenase with an Oxidase for the Synthesis of Aromatic Aldehydes from Benzyl Alcohols
Authors: Ma Yunjian , Li Zongquan , Zhang Hao , Wong Vincent Kam Wai , Hollmann Frank , Wang Yonghua
Published: January 2023

Aromatic aldehydes are important aromatic compounds for the flavour and fragrance industry. In this study, a parallel cascade combining aryl alcohol oxidase from Pleurotus eryngii (PeAAOx) and unspecific peroxygenase from the basidiomycete Agrocybe aegerita (AaeUPO) to convert aromatic primary alcohols into high-value aromatic aldehydes is proposed. Key influencing factors in the process of enzyme cascade catalysis, such as enzyme dosage, pH and temperature, were investigated. The universality of PeAAOx coupled with AaeUPO cascade catalysis for the synthesis of aromatic aldehyde flavour compounds from aromatic primary alcohols was evaluated. In a partially optimised system (comprising 30 μM PeAAOx, 2 μM AaeUPO at pH 7 and 40 °C) up to 84% conversion of 50 mM veratryl alcohol into veratryl aldehyde was achieved in a self-sufficient aerobic reaction. Promising turnover numbers of 2800 and 21,000 for PeAAOx and AaeUPO, respectively, point towards practical applicability.
Comprehensive Insight into Tapetum-Mediated Pollen Development in Arabidopsis thaliana
Authors: Wei Shuaijie , Ma Ligeng
Published: January 2023

In flowering plants, pollen development is a key process that is essential for sexual reproduction and seed set. Molecular and genetic studies indicate that pollen development is coordinatedly regulated by both gametophytic and sporophytic factors. Tapetum, the somatic cell layer adjacent to the developing male meiocytes, plays an essential role during pollen development. In the early anther development stage, the tapetal cells secrete nutrients, proteins, lipids, and enzymes for microsporocytes and microspore development, while initiating programmed cell death to provide critical materials for pollen wall formation in the late stage. Therefore, disrupting tapetum specification, development, or function usually leads to serious defects in pollen development. In this review, we aim to summarize the current understanding of tapetum-mediated pollen development and illuminate the underlying molecular mechanism in Arabidopsis thaliana.
Immunotherapy: Recent Advances and Its Future as a Neoadjuvant, Adjuvant, and Primary Treatment in Colorectal Cancer
Authors: Yu Irene , Dakwar Anthony , Takabe Kazuaki
Published: January 2023

Immunotherapy in colorectal cancer (CRC) has made great strides within the past decade. Immune checkpoint inhibitors are a class of immunotherapy and have been shown to greatly improve patient outcomes in mismatch repair-deficient (dMMR) CRC. Now, they are part of the standard of care for this subset of CRC. Because of this, there has been a growing interest in the efficacy and timing of immunotherapy for other subsets of CRC, including locally advanced, metastatic, and microsatellite stable (MSS). In this review, we aim to examine the three main classes of immunotherapy for CRC—immune checkpoint inhibitors (ICIs), adoptive cell transfer therapy (ACT), and tumor vaccines—and discuss the most recent advances and future directions for each.
Madagascar Leaf-Tail Geckos (Uroplatus spp.) Share Independently Evolved Differentiated ZZ/ZW Sex Chromosomes
Authors: Pensabene Eleonora , Yurchenko Alona , Kratochvíl Lukáš , Rovatsos Michail
Published: January 2023

Geckos are an excellent group to study the evolution of sex determination, as they possess a remarkable variability ranging from a complete absence of sex chromosomes to highly differentiated sex chromosomes. We explored sex determination in the Madagascar leaf-tail geckos of the genus Uroplatus. The cytogenetic analyses revealed highly heterochromatic W chromosomes in all three examined species (Uroplatus henkeli, U. alluaudi, U. sikorae). The comparative gene coverage analysis between sexes in U. henkeli uncovered an extensive Z-specific region, with a gene content shared with the chicken chromosomes 8, 20, 26 and 28. The genomic region homologous to chicken chromosome 28 has been independently co-opted for the role of sex chromosomes in several vertebrate lineages, including monitors, beaded lizards and monotremes, perhaps because it contains the amh gene, whose homologs were repeatedly recruited as a sex-determining locus. We demonstrate that all tested species of leaf-tail geckos share homologous sex chromosomes despite the differences in shape and size of their W chromosomes, which are not homologous to the sex chromosomes of other closely related genera. The rather old (at least 40 million years), highly differentiated sex chromosomes of Uroplatus geckos can serve as a great system to study the convergence of sex chromosomes evolved from the same genomic region.
Utilization of Recycled Material for Producing Magnesia–Carbon Refractories
Authors: Moritz Kirsten , Brachhold Nora , Hubálková Jana , Schmidt Gert , Aneziris Christos G.
Published: January 2023

The partial replacement of primary raw materials for the production of refractories with recycled ones gains in importance, as it contributes to the conservation of natural resources, energy saving, and reduction in greenhouse gas emissions. In this work, the use of a magnesia–carbon (MgO-C) recyclate in the fractions 3–6, 1–3, and 0–1 mm as a raw material for MgO-C refractories was investigated. The recyclate was examined via optical and scanning electron microscopy. Using mixtures with different recyclate contents up to 82 wt%, MgO-C specimens were prepared to study the influence of the recyclate on their chemical composition, structure, and properties. The substitution of primary raw materials with the recyclate did not cause a decrease in the MgO content analyzed after carbon burnout, but the contents of the individual impurities changed. A comparison of the MgO-C that contains 82 wt% recyclate with the recyclate-free material through computed tomography indicated a change in the size distribution of the MgO grains. The porosity increased, and the cold modulus of rupture decreased with increasing recyclate content, whereas the thermal shock resistance improved. At a recyclate content of 40 wt%, the refractoriness under load was only slightly lower than that of the recyclate-free material.