bluuebunny/crossref_metadata_2025 huggingface dataset:
Marketing strategy analysis based on segmenting and targeting in hospital: A literature review
Authors: Mufakhhir Abdulaziez , Jati Sutopo Patria , Arso Septo Pawelas
Published: February 2024

Background: Healthcare services have undergone changes in several strategic business units that require proper management handling. The evolution of hospitals, accompanied by the high demands of the community for services, has led hospitals to compete in seeking new opportunities and strategies to meet consumer (patient) needs. In an effort to meet consumer expectations and desires, hospitals need to design a marketing mix that provides value to the target consumers. Management planning serves the target market based on hospital market segmentation.Purpose: To define the analysis of healthcare service market segments in a manner that supports the marketing process in hospitalsMethod: This study is a literature review. The research data sources were derived from literature obtained via the internet, including scientific research findings from various sources. The population in this study consisted of research focusing on the assessment of hospital market segmentation from 2015 to 2022. A total of 12 research articles were obtained from scholarly publications such as Science Direct, Scopus, ProQuest, Springer Link, Google Scholar, PubMed, JSTOR, and Emerald Insight. The collected data were then systematically analyzed.Results: This result revealed that a marketing strategy employing segmentation concepts, supported by an appropriate marketing mix, was an effective means to enhance healthcare services according to customer needsConclusion: This study concluded that hospital market segmentation analysis, based on geographic, demographic, psychographic, and behavioral factors and supported by an appropriate marketing mix, was an effective means to improve healthcare services according to customer needs.
An overview of treatment for atrial septal defect with pulmonary hypertension
Authors: Gudi Veronika Anjelina Aho , Suastika Luh Oliva Saraswati , Sinardja Cyndiana Widia Dewi
Published: February 2024

Background: Atrial Septal Defect (ASD) is a congenital heart defect which can be the cause of comorbidities in the form of Pulmonary Arterial Hypertension (PAH) and therapy cannot be done simply by correcting the ASD but requires further study and evaluation.Purpose: Describe the treatment given to patients with ASD and PAH and the correlation between the administration of interventional therapy and the patient's probability of PH.Method: This study is a descriptive quantitative using a cross-sectional research design. The sampling technique was total sampling method. The samples were 41 ASD patients with PAH at Prof. Ngoerah General Hospital in Denpasar in 2022. Patients under 18 years of age and those with other congenital heart disease will be excluded. The demographic characteristics assessed were the patient's age and gender. The therapy data included information on medical therapy and intervention therapy.Results: Focus of medical therapy was supportive therapy rather than specific PAH therapy. Percutaneous ASD closure was the most widely-used intervention therapy compared to surgical ASD closure. Based on statistical analysis, the p value > 0.05 is 0.284, so there is no significant correlation between the probability of the patient's pH and the provision of intervention therapy.Conclusion: At Prof Ngoerah General Hospital Denpasar, the majority of ASD patients with complications of pulmonary hypertension were females aged between 21 to 30 years. The medical therapy given is adjusted based on the severity of PH as assessed through echocardiography examination. Majority of patients received supportive therapy compared to specific therapy for PAH. Medical therapy is prioritized for patients who have contraindication criteria for interventional therapy. Percutaneous ASD Closure was found to be the largest percentage in interventional therapy regardless of the severity, except for patients who were indicated to undergo surgical ASD closure. There is no significant relationship between the patient's PH probability level and the choice of intervention therapy.
Determinants factors on Public Health Centre nurses' confidence in performing cardiopulmonary resuscitation
Authors: Sasmito Priyo , Purnamasari Elly , Sumartini Sri , Purwanti Nunuk Sri , Budiyati Budiyati , Putra Riskiyana Sukandhi , Arifani Nisa , Elviani Yeni
Published: February 2024

Background: Most cardiac arrests occur outside the hospital (out-of-hospital cardiac arrest/OHCA). Public Health Centre (PHC) nurses are one of the important components of the chain of survival. The confidence of PHC nurses in performing Cardiopulmonary resuscitation (CPR) is still questioned.Purpose: To analyze the factors determining the level of confidence of PHC nurses in performing CPR.Method: An observational analytical study with a cross-sectional approach involving 30 nurses from 2 PHCs in Banten Province, Indonesia taken using a total sampling technique.Results: A total of 30 participants participated in this study. The average age of participants was 36.4 years with an age range between 26-55 years. Most participants were male 17/56.7%, had a diploma education background of 21/70.0%, and had upgraded their BLS training 23/76.7%. On average, participants had worked for 6.5 years and managed 3.4 cases of cardiac arrest (CA) during their time as nurses. It was found that gender, educational background, and BLS training status were not associated with the nurse's level of confidence in performing CPR. While employment status has a significant relationship with confidence in performing. Age has a strong relationship with self-confidence with an r rank value of 0.447. Length of work also showed a relationship with self-confidence with an r-rank value of 0.503. Likewise, the number of CA cases ever managed has a relationship with self-confidence with an r rank of 0.419.Conclusion: Gender, educational background, and BLS training status were not associated with nurses' confidence levels in performing CPR. Employment status, age, length of work, and number of CA cases managed were associated with nurses' confidence in performing CPR.
Judicial Accountability: A Comparative Note on India and Malaysia
Authors: Shri Rangaswamy D
Published: February 2024

India and Malaysia are the countries having similar characteristics of the political system. The self-governed model of Judicial Accountability has been given impetus importance in Malaysia contrary to the Indian legal system. The Judicial Standards and Accountability Bill 2012 being a replica of the Judges Enquiry (Amendment) Bill 2005, has been pending before the Parliament for many years. On the other hand, the Malaysian legal system adopted the judicial standards way back in 1986. Inordinate delay on the part of the Indian legal system seems to be a major setback for the image of the biggest democratic country. The severe allegations against judges of the Supreme Court of India and High Courts and aborted efforts of the Parliament in ensuring judicial accountability have inspired the author to take up a comparative study of the topic. The paper starts with a cursory view of the judicial system in India and Malaysia followed by a conceptual analysis of judicial accountability. It outlines the status of judicial accountability as contemplated under the constitutional and legal framework of both countries. It concludes by identifying the similarities, dissimilarities, and best practices of both systems in the context of ideal practices required for judicial accountability.
Security Challenges in the Application of Blockchain Technology in Energy Trading
Authors: Karisma Karisma
Published: February 2024

This paper examines the interplay between blockchain technology and the energy sector, focusing on security limits, barriers, and challenges. The authors discusses the primary components of cyber risks, including threats, vulnerabilities, and impacts that plague blockchain systems and their application, network, and data layers. Further, anonymity is a key feature of blockchain, ensuring that blockchain users, nodes, and miners remain unidentifiable by any measure. Therefore, perpetrator-focused measures are not viable when assigning responsibility for dangerous and illegal conduct. There are concerns that the concealment of identity will broaden blockchain attack surfaces and pose risks to energy security. The authors also emphasises the need for a well-defined and consistent legal and regulatory framework to address the complexities of blockchain development in the energy sector and assert that the maturity of blockchain in this industry will depend on balancing security and user rights and suggest implementing ex-ante and ex-post measures. This paper is novel; the author seeks to provide an in-depth analysis of the security challenges faced by blockchain-based energy applications and offer practical solutions for mitigating these cybersecurity threats and vulnerabilities.
How does earnings management impact annual report readability?
Authors: Dau Nam Huong , Nguyen Duy Van , Diem Hai Thi Thanh
Published: February 2024

This research investigates the relationship between earnings management and annual report readability for companies listed on the Singapore Stock Exchange (SGX). Research on earnings management (EM) and the ability to read the annual report play a crucial role for investors as well as other stakeholders. The extent of earnings management can be determined based on annual reports. The analysis is based on the data of 251 domestic companies listed on the SGX from 2016 to 2021. Regression analysis of panel data was conducted using STATA version 15 software in this research. The result shows that companies with more aggressive earnings management tend to make annual reports more complex and, hence, less readable. In addition, the study also shows that the impact of earnings management on the annual report readability for enterprises of different sizes, profits, ages, and levels of financial distress is the same. Theoretically, the findings of this research for a developed financial market in Asia (Singapore) further confirm and reinforce the findings about the relationship between earnings management and report readability. In addition, one may draw important practical implications for investors as well as stakeholders in general; in particular, stakeholders may have more evidence for suspicions about earnings management whenever the annual reports become more difficult to read and understand.
Chronic Neck Swelling: A Case Report of Migrating Fish Bone
Authors: BIN AHMAD HUSNI SAIFUL ISLAM , Binti Azman Mawaddah , Timothy Wong Leong Wei
Published: February 2024

Background: Foreign bodies in the upper aerodigestive tract are frequently seen in otolaryngological practice, but migration of foreign body and oesophageal penetration are rare occurrences. Most reported cases of migration in literature involve fish bones, which are usually found in the lateral neck soft tissue or thyroid lobule. This can cause serious complications if left untreated. In most cases, fish bones can be removed safely by endoscopy, but they may migrate extraluminally to the skin in rare cases. Computed tomography (CT) neck with contrast is the investigation of choice to confirm migration.
Case presentation: We present a case of an elderly lady with fish bone ingestion which migrated and embedded itself over the right thyroid cartilage region that required open surgery for removal. In this case, the patient underwent two open surgeries to locate the presence of the foreign body despite the CT neck done to aid in mapping intraoperatively. We used surgical clips in the first unsuccessful operation in locating the foreign body, and later performed a repeat CT scan and the clips were used to pinpoint the exact location of the foreign body during second surgery. By doing so, we successfully managed to remove the foreign body and patient was discharged well.
Conclusion: This case has illustrated how versatile surgical clips are in helping us to locate the position of the foreign body rather than blindly exploring and causing further iatrogenic injury. With supplementation from a thorough repeat CT scan, we do think that in a presenting case of foreign body migration with difficulty locating the exact foreign body location, imaging adjunct such as C arm fluoroscopy and ultrasound guidance as reported in the literature can be used to avoid unnecessary iatrogenic injury and cause more harm to the patient.
تقييم أساليب التفكير وفق انموذج استيرنبرغ ونسب مساهمتها في التعقل النفسي لدى لاعبي كرة اليد في الدوري العراقي الممتاز
Authors: Nour Hazem Anaid  , Prof. Dr. Mithaq Ghazi Muhammad
Published: February 2024

وقد هدفت الدراسة الى اعداد مقياس اساليب التفكير وفق نظرية أستيرنبرغ لدى لاعبي كرة اليد في الدوري العراقي الممتاز ، وبناء مقياس التعقل النفسي لدى لاعبي كرة اليد في الدوري العراقي الممتاز ، واعداد مقياس التوافق الذاتي والاجتماعي للاعبي كرة اليد في الدوري العراقي الممتاز واستخدما الباحثتان المنهج الوصفي الذي يحظى بمكانه خاصة في مجال البحوث النفسية  وقد تمثل مجتمع البحث بلاعبي كرة اليد في الدوري العراقي الممتاز للموسم الرياضي ( 2022-2023) والبالغ عددهم ( 243 )  لاعبا يمثلون (15) ناديا ،وقد توصلا الباحثتان الى عدة استنتاجات أهمها للمقاييس المعدة والمصممة من قبل الباحثة القدرة على قياس كل من اساليب التفكير والتوافق الذاتي والاجتماعي والتعقل النفسي لدى لاعبي كرة اليد في الدوري العراقي الممتاز ، لأساليب التفكير (المحافظ , المحلي , الهرمي , الحكمي , التشريعي والخارجي علاقات ارتباط ذات دلالة احصائية عالية تباعا بالتعقل النفسي لدى عينة البحث .
ابعاد الاحتراق النفسي وعلاقته بالصلابة النفسية للاعبي اندية الدرجة الممتازة بكرة اليد
Authors: Hoda Alaa Manea  , Prof. Dr.. Abdul Abbas Abdul Razzaq
Published: February 2024

تكمن أهمية هذه الدراسة أن ظاهرة الاحتراق النفسي هي ظاهرة نفسية معرقلة للإنجاز الرياضي تودي بمستوى لاعبي كرة اليد بالدوري الممتاز لان هذه الظاهرة تتميز بأبعاد عديدة ممكن تؤثر بعضها بمستوى لاعبي كرة اليد بالدوري الممتاز بسبب تقارب المستويات نوعا ما بين الفرق وارتفاع حده المنافسة مما أدى الى التركيز على الجوانب التدريبية أو الفنية، اما مشكلة البحث تتبلور المشكلة على مجموعة من التساؤلات التي تحدد أبعاد المشكلة وتحيط بموضوع اختيار هذه الدراسة وتحاول وضع الحلول الناجعة اليها وهي:

ما هي أهمية معرفة عدد من الظواهر النفسية والتداخل فيما بينها ومدى تأثيرها على لاعبي كرة اليد و

هل هنالك علاقة بين موضوعات الدراسة ومدى تأثرها ببعض وتأثيرها على للاعبي كرة اليد للدوري الممتاز.

قلة الدراسات التي تناولت موضوع الاحتراق النفسي وربطها بموضوعات كصلابة النفسية للاعبي كرة اليد بالدوري الممتاز.

ما هو دور الاحتراق النفسي على لاعبي كرة اليد ومدى دور الصلابة النفسية للاعبين بالدوري الممتاز للحد من ذلك.

وكانت اهداف البحث هي بناء وتطبيق مقياس أبعاد الاحتراق النفسي لدى لاعبي أندية الدرجة الممتازة للكرة اليد ،و أعداد وتطبيق مقياس الصلابة النفسية لدى لاعبي أندية الدرجة الممتازة بكرة اليد ، و وضع مستويات معيارية لمقياس أبعاد الاحتراق النفسي والصلابة النفسية لدى لاعبي أندية الدرجة الممتازة لكرة اليد ، والتعرف على مستوى أبعاد الاحتراق النفسي والصلابة النفسية لدى لاعبي أندية الدرجة الممتازة لكرة اليد ، والتعرف على العلاقة بين أبعاد مقياس الاحتراق النفسي والصلابة النفسية لدى لاعبي أندية الدرجة الممتازة في كرة اليد .
Penggunaan Metode Suku Kata Untuk Meningkatkan Hasil Belajar Membaca Pada Mata Pelajaran Bahasa Indonesia Siswa Kelas 1 SDN Tlogomas 2 Kota Malang
Authors: Firsta Bagus Sugiharto  , Chusnul Chotimah  , Devi Dominika
Published: February 2024

The purpose of  study was to describe the level of student learning in reading learning outcomes in Indonesian subjects using the syllable method for first-grade students at SDN Tlogomas 2 Malang City. This study used the Classroom Action Research (PTK) method, which consisted of two cycles, each cycle held 2 class meetings. The approach in this research is descriptive qualitative. The subjects of this study were all first-grade students at SDN Tlogomas 2 Malang City   totaly of 20 of them 11 male students and 9 female students. Data collection techniques in this study used observation, interviews, and documentation. The results showed that: (1) the application of the Syllable learning method in cycle 1 meeting 1 presentation 65.00% with sufficient category, the second meeting 75.00% with good category, and in the implementation of cycle II meeting one presentation 85.00% with good category, second meeting 92.05% with very good category. (2) the improvement of Indonesian learning outcomes by using the syllable learning method in Indonesian subjects, cycle 1 meeting one presentation was 50% completeness, with an average score of 67.5%, while in the second meeting, the presentation was 60% completeness, with an average score 69% than in the implementation of cycle II experienced an increase at the first meeting the percentage of completeness was 70% with an average value of 79.5%, and at the second meeting the percentage of completeness was 80% with an average value of 85%. Therefore it can be concluded that using the syllable method in the learning process it can increase the activity and learning outcomes of Indonesian students in class I SDN Tlogomas 2 Malang.
Implementasi Pelaksanaan Program Guru Penggerak Terhadap Kinerja Guru di SDN Bhayangkari Kota Serang
Authors: Nur Anisa  , Trian Pamungkas Alamsyah  , Ahmad Syachruroji
Published: February 2024

Pada penelitian ini bertujuan untuk mengetahui pelaksanaan program guru penggerak terhadap kinerja guru di SDN Bhayangkari Kota Serang, kemudian untuk mengetahui kinerja guru penggerak menghadapi kendala dalam memimpin rekan kerja guru serta hasil dari adanya program guru penggerak di SDN Bhayangkari Kota Serang. Metode yang digunakan pada penelitian ini kualitatif dengan jenis kualitatif deskriptif. Pengumpulan data pada penelitian ini menggunakan teknik observasi, wawancara dan dokumentasi. Kemudian dianalisis menggunakan model Miles and Huberman. Hasil penelitian ini dapat mengungkapkan bahwa dari pelaksanaan guru penggerak di SDN Bhayangkari Kota Serang memberikan banyak perubahan dan perkembangan pada sekolah melalui program-program yang dirancang oleh guru penggerak untuk menjalankan budaya positif di sekolah bersama rekan guru, guru penggerak juga memiliki kinerja yang baik melalui program yang telah dijalankan dan dalam memimpin dan membantu rekan kerja guru kelas 1, 2, 4 dan 5 melaksanakan kurikulum merdeka dan pelaksanaan coaching serta dari implementasi program guru penggrak di SDN Bhayangkari Kota Serang memberikan banyak perubahan dan perkembangan di sekolah, sehingga dapat disimpulkan bahwa program guru penggerak ini sangat baik untuk pekembangan dan kemajuan sekolah sehingga dengan adanya program guru penggerak ini menjadi evaluasi bagi program guru penggerak di sekolah maupun bagi program itu sendiri.
Pengembangan LKPD dengan Pendekatan Saintifik dalam Melatih Keterampilan 4C di Kelas V SD
Authors: Mega Mulia  , A. Syachruroji  , Nana Hendracipta
Published: February 2024

Penelitian dan pengembangan ini bertujuan untuk mengetahui cara pengembangan, kelayakan dan respon peserta didik terhadap LKPD dengan pendekatan saintifik dalam melatih keterampilan 4C di kelas V SD dalam tema 2 subtema 1 pembelajaran ke-2. Penelitian ini menggunakan metode penelitian dan pengembangan (R&D) dengan desain penelitian research and development oleh Borg & Gall. Subjek pada penelitian ini adalah 28 peserta didik kelas V SD Negeri Sukabela Kota Serang tahun ajaran 2023/2024 dengan menggunakan teknik purposive sampling. Berdasarkan hasil validasi produk, dari ahli materi memperoleh rerata keseluruhan skor persentase 92% dengan kriteria sangat layak, validasi ahli media sebesar 89,5% dengan kriteria sangat layak, dan validasi ahli bahasa sebesar 83% dengan kriteria layak. Maka keseluruhan persentase kelayakan produk mencapai 88,16% dengan kriteria sangat layak, serta respon peserta didik memperoleh rerata skor persentase 98,1% dengan kriteria sangat baik. Kesimpulannya LKPD dengan pendekatan saintifik dalam melatih keterampilan 4C di kelas V SD sangat layak dan sangat baik untuk digunakan dalam proses pembelajaran peserta didik di kelas V SD.
Leading Remote Organizations Strategies for Managing Effective Knowledge Sharing Within Teams
Authors: McFadden Jessica , McClain William Lee
Published: February 2024

Remote teams have become an integral part of the operations of global organizations in an increasingly digital era. The complexities of knowledge sharing and management within teams were explored. A focus was placed on the leadership practices that influenced complexities. Using qualitative insights from participants, the importance of transformational leadership in fostering a culture of open communication, trust, and collaboration in remote settings was emphasized in the study. The findings indicated that hat leaders who showed transformational traits such as charisma, authenticity, intellectual stimulation, and individualized consideration enhanced knowledge-sharing within their teams. Leaders’ practices promoted knowledge sharing and positively impacted team productivity, job satisfaction, and retention. In remote work, transformational leadership was pivotal in ensuring effective knowledge management, underscoring its importance for contemporary organizations aiming for growth and innovation in a digital landscape.
Note for the Millennium Prize Problems
Authors: Vega Frank
Published: February 2024

The Riemann hypothesis and the $P$ versus $NP$ problem are two of the most important unsolved Millennium Prize Problems. Let $\Psi(n) = n \cdot \prod_{q \mid n}\left(1 + \frac{1}{q} \right)$ denote the Dedekind $\Psi$ function where $q \mid n$ means the prime $q$ divides $n$. Define, for $n \geq 3$; the ratio $R(n) = \frac{\Psi(n)}{n \cdot \log \log n}$ where $\log$ is the natural logarithm. Let $N_{n} = 2 \cdot \ldots \cdot q_{n}$ be the primorial of order $n$. We prove if the inequality $R(N_{n+1}) < R(N_{n})$ holds for all primes $q_{n}$ (greater than some threshold), then the Riemann hypothesis is true. In this note, we show that the previous inequality always holds for all large enough prime numbers. $P$ versus $NP$ is considered as one of the most fundamental open problems in computer science. This consists in knowing the answer of the following question: Is $P$ equal to $NP$? It was essentially mentioned in 1955 from a letter written by John Nash to the United States National Security Agency. However, a precise statement of the $P$ versus $NP$ problem was introduced independently by Stephen Cook and Leonid Levin. Since that date, all efforts to find a proof for this problem have failed. Another major complexity class is $NP$-complete. It is well-known that $P$ is equal to $NP$ under the assumption of the existence of a polynomial time algorithm for some $NP$-complete. We show that the Monotone Weighted Xor 2-satisfiability problem ($MWX2SAT$) is $NP$-complete and $P$ at the same time.
INTRODUCTION ISSUES: INFLUENCE OF SALINITY ON GAS EXCHANGE PROCESSES OF SEEDLINGS HEDYSARUM GRANDIFLORUM PALL.
Authors: Dumacheva Elena , USOL'CEVA Elena , MAKSIMOVA Polina , GAAR Aleksandr , SOPIN Denis , BIYuShKINA Anna , AKIMOV Aleksandr
Published: February 2024

The large-flowered sweetvetch (Hedysarum grandiflorum Pall.) is a perennial beautiful-flowering plant with a narrow ecological amplitude, promising for introduction and cultivation. Under controlled conditions using the LI-6800 instrument, LI-COR, USA, the dynamics of indicators of photosynthesis intensity, transpiration and accompanying physiological processes related to gas exchange in H. grandiflorum seedling leaves under different light levels (1500, 1000, 500, 200, 100, 50, 0 µM/m2/s) in saline conditions was studied. It has been established that photosynthetic apparatus of H. grandiflorum seedlings adapts well enough to saline conditions and significant photosynthesis suppression by 17.4 % is observed only at PAR level of 300 µM/m²/s¹. At the same time, a significant decrease in the rate of water evaporation by the surface of cotyledon leaves under the influence of salinity conditions — by 36.5–54.3 % is observed. The obtained data indicate a potentially high adaptive capacity of H. grandiflorum seedlings due to self-regulation of gas exchange processes.
EFFECT OF MINERAL FERTILIZERS ON THE BOTANICAL COMPOSITION, QUALITY AND PRODUCTIVITY OF PASTURE HERBAGE
Authors: Kovshova Valentina , Smirnova Anna , LUZYaNINA Nadezhda
Published: February 2024

The role of mineral fertilizer of a long-term pasture herbage located on a low-lying drained peat bog in preserving the valuable composition of the herbage, increasing its quality characteristics and productivity over a long period (88 years) is shown without additional capital investments for improvement.
ENZYME ACTIVITY IN DEVELOPED PEAT SOILS
Authors: Lobanova Ekaterina , POROHINA Ekaterina
Published: February 2024

The article presents the results of a study of the activity of some enzymes from the class of oxidoreductases in the developed peat soils of the Tagan swamp. It was found that the entire profile of the studied soils is biochemically active, which ensures their potential fertility.
PRODUCTIVITY OF HERBS IN THE PRODUCTION OF HAYLAGE IN THE CONDITIONS OF THE MOSCOW REGION
Authors: DUBOREZOV Ivan , Kosolapov Andrey , Duborezov Vasily
Published: February 2024

As a result of tests of eight variants of grass mixtures of annual fodder crops, the most productive combination of them was revealed. In particular, from two-component mixtures, higher productivity was provided by oat crops with vetch or peas in a combination of 1:1.5. Their productivity per hectare amounted to more than one ton of crude protein and more than 45 GJ of metabolic energy. At the same time, the nutritional value of haylage (45% of dry matter) in these variants, estimated by protein content and energy value (96 and 94 grams and 0.40 and 0.41 of energy feed unit, respectively), had higher indicators compared to grass mixtures in variants where the proportion of oats exceeded the proportion of legumes by one and a half times. The addition of mustard and sunflower to the herb mixture (about 5% of each crop from the seeding rate) allows you to get a feed of the same nutritional value, but with greater productivity per unit area of sowing. In particular, in these variants, 1346 and 1370 kg of crude protein, 61.7 and 57.6 GJ of metabolic energy were obtained from 1 ha. An increase in the proportion of sunflower increases the yield of vegetative mass (due to increased humidity), but leads to a decrease in productivity.
COMPARATIVE ANALYSIS OF THE RATE OF CO2 ASSIMILATION IN ANNUAL TRIFOLIUM SPECIES AT THE JUVENILE STAGE OF ONTOGENESIS
Authors: Dumacheva Elena , Kostenko Sergey , Chernyavskih Vladimir , PEChEGINA Yuliya , Grebennikov Artem
Published: February 2024

The parameters of photosynthesis, dark respiration and intercellular CO2 concentration were studied in three species of annual clovers: Trifolium resupinatum, T. alexandrinum, T. incarnatum. Significant differences in the parameters of the rate of carbon dioxide assimilation between the studied species were established in the experiment. The maximum value of dark respiration and CO2 assimilation rate was observed in the seedling leaves of T. alexandrinum seedlings. The equations of light curves of CO2 assimilation and intercellular CO2 concentration were calculated. High approximation coefficients were found: from 0.945 to 0.989 for photosynthesis light curves; from 0.683 to 0.983 for intercellular CO2 concentrations.
EFFICIENCY OF SYMBIOSIS OF NEW STRAINS OF NODULE BACTERIA WITH ALFALFA VARIABLE
Authors: Ionov Aleksey , Stepanova Galina
Published: February 2024

Three active strains of nodule bacteria were created Pre-sowing inoculation with these strains increased the productivity of alfalfa plants of the Taisiya variety by an average of 23–34 % over six accounting cycles. Strains I and IV isolated from the nodules of variable alfalfa increased the productivity of inoculated alfalfa plants by 11–55 and 32–56 percent. It was found that strain IV is relatively tolerant to high air and substrate temperatures (30–34 ºC). The effectiveness of symbiosis with strain II isolated from the nodules of the melilot white was in the range of 17–51 % by mowing. A negative reaction of strain II to an increase in air and substrate temperature above 28–30 ºC was noted.
AGROECOLOGICAL ASSESSMENT OF CULTIVATION OF NARROW-LEAVED LUPINE FOR GREEN FODDER
Authors: Emelev S. , Lybenko Elena
Published: February 2024

The article presents an agroecological assessment of the cultivation of low-alkaloid varieties of narrow-leaved lupine for green fodder in the conditions of the Kirov region. It was found that the most productive varieties in terms of green mass were Belorozovy 144 (75.0 t/ha) and Bryanskiy kormovoy (71.4 t/ha). The average dry matter content of the varieties of narrow-leaved lupine was 16.7%, which is lower than the controls by 10.8 and 6.8%, respectively. According to the results of the analysis of the crude protein content in the green mass of legumes, it was found that this indicator is 19-34% higher in lupine varieties than in peas. In comparison with peas, the Belorozovy 144 variety (3,9) has a higher coefficient of energy efficiency. In the Bryanskiy kormovoy variety, this indicator is at the level of peas (3.8). These two varieties can be recommended to increase the share of annual legumes in crop rotation.
FEATURES OF CROP FORMATION BY SOYBEAN VARIETIES OF SELECTION OF THE RYAZAN RESEARCH INSTITUTE IN THE CONDITIONS OF THE KALUGA REGION
Authors: HRAMOY Viktor , Siharulidze Tamila
Published: February 2024

The formation of the crop by four varieties of soybeans has been studied: Mageva, Svetlaya, Kasatka, and Maleta. The conducted studies have shown that in some years extremely unfavorable weather conditions for soybeans develop in the Kaluga region, which leads to an extension of its growing season and a decrease in seed yield. The Svetlaya soybean variety turned out to be the most adapted to such conditions, the seed yield of which averaged 1.63 t/ha over 2 years. In the Mageva variety, the yield was lower by 24.4%; in the Kasatka and Maleta varieties it was lower by 29.9%.
CREATION OF THE ORCHARD GRASS VARIETY (Dactylis glomerata L.) FOR THE NON-CHERNOZEM ZONE OF THE RUSSIAN FEDERATION
Authors: Kostenko Natalia , Kostenko Sergey , KUZ'MIN Evgeniy , Chernyavskih Vladimir
Published: February 2024

The orchard grass is one of the main fodder crops in the European part of Russia. However, in recent years, there has been a noticeable shortage of seeds of this crop on the market, mainly due to the elimination of small seed plots in most farms and the lack of a sufficient number of new varieties of this crop.
MEADOW TIMOTHY. BREEDING SPECIALIZED VARIETIES FOR HAYFIELDS, PASTURES AND TURFS
Authors: Kostenko Natalia , Kostenko Sergey , Malyuzhenets Evgenia
Published: February 2024

Timothy grass began to be used as a fodder crop in the north of the Non-Chernozem zone of Russia more than 400 years ago, most often it was sown together with meadow clover. These two species have quite similar biorhythms of development. Then this culture began to spread widely, first in Europe, and then around the world. The main advantages of Timothy grass are high ecological plasticity, good adaptability to heavy soils, and longevity. Modern varieties are characterized by good resistance to diseases, adverse weather conditions and relatively good quality indicators of the resulting feed.
WHEATGRASS IS THE MOST IMPORTANT FIELD CROP IN RUSSIA
Authors: Saprykin Sergey , Saprykina Natal'ya , Lyubtseva Olga
Published: February 2024

The results of the study in 2022–2023 of selective specimens of crested wheatgrass in the nursery of preliminary varietal testing. Based on the results of the study five varietal samples were identified — SP-1, SP-12, SP-13, SP-20 and SP-22, which significantly exceeded the standard — variety Pavlovsky 12: in yield of green mass — by 6.9–43.9 centners/ha, dry matter — by 0.6–13.4 centners/ha, seeds — by 0.2–2.4 centners/ha. The best in the experiment was the selection sample SP-22, which exceeded the standard in terms of green phytomass collection — by 35.7 %, dry matter — by 33.2 %, seeds — by 58.5 %. The selected varietal samples will be further tested for economically valuable traits in the nursery of competitive variety testing.
SELECTION OF VARIETIES OF HYBRID CLOVER (Trifolium hybridum L.) FOR CONDITIONS KALININGRAD REGION
Authors: Dumacheva Elena , Zarudnyy Vladimir , Kostenko Sergey , Krasnoperov1 Andrey , Pyatakov Maksim
Published: February 2024

The climatic conditions of the Kaliningrad region put forward specific requirements for agricultural crops that can show the greatest productivity in these conditions and significantly increase the efficiency of agricultural production in general. Therefore, the varieties of forage crops that will be most suitable for this region must be created taking into account the regional approach.
MOBILIZATIONS OF GENETIC RESOURCES OF PERENNIAL AND ANNUAL GRASSES IN THE CRETACEOUS SOUTH OF THE CENTRAL RUSSIAN
Authors: Dumacheva Elena , GONChAROVA Natal'ya , KALAShNIKOVA Viktoriya , PEChEGINA Yuliya , PEChEGIN Aleksandr , Grebennikov Artem , SOPINA Natal'ya
Published: February 2024

The article discusses the results of joint research by scientists and graduate students of the V. R. Williams Federal Scientific Centre for Forage Production and Agroecology and Belgorod State National Research University on the search and selection of valuable sources of breeding traits and mobilization of plant genetic resources. V. R. Williams and Belgorod State National Research University on search and selection of valuable sources of breeding traits and mobilization of plant genetic resources on the territory of the Cretaceous south of the Central Russian Upland. As a result, the sources of valuable economic traits of annual and perennial grasses (alfalfa sickle, large-flowered sweetvetch, oriental goats-rue grass, strawberry clover, phacelia tansy-leaved and others) were identified in terms of seed productivity and productivity of aboveground phytomass, cladding, duration of flowering period.
EXPERIÊNCIAS EM PRODUÇÕES AUDIOVISUAIS E EDUCAÇÃO
Authors: Franco Marilia , Stecz Solange Straube , Veiga André Barroso da , Cesar Bruno Nascimento da Silva , Silveira Estevan da , Campos Márcia Regina Galvan , Assunção Morgana Sousa , Rodrigues Jr. Odair , Santos Tania Maria dos
Published: February 2024

Artigo coletivo realizado para a disciplina de "Mí­dias audiovisuais, recepção, expressão e educação" , do Programa de Pós-Graduação Mestrado Profissional em Artes, da Universidade Estadual do Paraná, Campus de Curitiba II, no ano de 2019. No referido texto, os autores fazem um levantamento de sete práticas que se relacionam com conceitos de Cinema e Educação. O texto analisa experiências realizadas na cidade de Curitiba, no estado do Paraná, no Brasil e em paí­ses da América Latina.
O TEATRO COMO SUBSTANTIVO CONCRETO DO SENSÍVEL: BREVE DIÁLOGO PARA UMA METODOLOGIA INCOMPLETA
Authors: Dominoni Thiago
Published: February 2024

O artigo apresenta a prática de ensino, aprendizagem e pesquisa em teatro na criação da primeira Companhia de Teatro da Biblioteca Pública do Paraná, situada em Curitiba. Descreve-se aqui os quatro anos de funcionamento da Companhia apreendendo as processualidades desta prática e como durante este perí­odo localiza a sobrevivência de uma morte contí­nua de aprendizados. Com isso identifica as etapas do processo metodológico como a transição de uma curiosidade ingênua para uma curiosidade epistemológica, isto é, acentua e persegue a autonomia da criação, pensando a docência como um compromisso com a vulnerabilidade do saber, mantendo-o vivo e operante às mudanças provocadas por cada encontro dado à prática. Esta premissa é compreendida como um artí­fice fundamental para organicidade desta rede de reciprocidades. Para isso o texto vê a docência como prática de mediação cultural e artí­stica. Por esta tessitura, pensa a relação pedagógica do teatro para a construção de uma metodologia criada para desmanchar-se, e assim, capaz de movimentar sua atividade reflexiva para um encontro de novas perguntas, ciclicamente.
MEDIAÇÃO TEATRAL PARA ESPETÁCULO DE RUA: ESPAÇO PARA (A)COLHER AFETOS
Authors: Carlim Victor Emanuel , Rosseto Robson
Published: February 2024

O presente artigo apresenta uma reflexão com base em propostas de mediação teatral desenvolvidas com espectadores transeuntes no espetáculo de rua "Hi, Breasil" , do grupo de teatro Olho rasteiro, realizada em novembro de 2019, na cidade de Curitiba. Partindo de estudos sobre mediação teatral, experiência artí­stica, afeto e espectadores transeuntes, este artigo compreende a mediação teatral como uma experiência potencial para mobilizar as capacidades perceptivas do espectador. Nesse sentido, para além do acesso a obra artí­stica, após o espetáculo assistido, o espectador transeunte teve a possibilidade para expressar, de maneiras diversas, as questões suscitadas pelo espetáculo, ao adentrar o espaço cênico e terem suas reflexões e percepções acerca do espetáculo registradas em ví­deo. O resultado permitiu discutir as interações dos espectadores a partir dos procedimentos metodológicos de mediação aplicados e o papel do mediador na efetivação de um espaço de acolhimento aos transeuntes, promovendo reflexões, partilhas, provocações e afetos.
Dança Na Escola: Apreciar, Experimentar E Refletir
Authors: Alves Erika Kraychete
Published: February 2024

Esta resenha crí­tica intenta apresentar o livro Por uma escola que dança(2019) das autoras: Isleide Steil e Adair de Aguiar Neitzel, contendo 108 páginas e publicado pela Editora CRV (Curitiba) em 2019. A obra reflete sobre possibilidades de se entender, processar, projetar e aplicar Dança – como área de conhecimento autônoma – no ensino básico. A escrita se reveste dos resultados de uma pesquisa realizada por ambas as autoras utilizando-se de uma metodologia especí­fica, a PEBA – Pesquisa Educacional Baseada em Arte: A/r/tografia em que acompanharam estudantes que participaram de aulas de dança no contraturno de uma escola de Santa Catarina.
A novel cartilage-targeting MOF-HMME-RGD sonosensitizer combined with sonodynamic therapy to enhance chondrogenesis and cartilage regeneration
Authors: Luo Shanchao , Shang Yifeng , Qin Zainen , Zhou Bo , Lu Chun , Qu Yangyang , Zhao Jinmin , Liang Ruiming , Zheng Li , Luo Shixing
Published: February 2024

Articular cartilage regeneration is still a difficult task due to the cartilage’s weak capacity for self-healing and the effectiveness of the available therapies. The engineering of cartilage tissue has seen widespread use of stem cell-based therapies. However, efficient orientation of line-specific bone marrow mesenchymal stem cells (BMSCs) to chondrogenesis and maintenance of chondrogenic differentiation challenged stem cell-based therapy. Herein, we developed a Fe-based metal-organic framework (MOF) loaded with hematoporphyrin monomethyl ether (HMME) and cartilage-targeting arginine-aspartate-glycine (RGD) peptide to form MOF-HMME-RGD sonosensitizer to regulate BMSCs chondrogenic differentiation for cartilage regeneration via the modulation of reactive oxygen species (ROS). By using sonodynamic therapy (SDT), the MOF-HMME-RGD demonstrated favorable biocompatibility, could generate a modest amount of ROS, and enhanced BMSCs chondrogenic differentiation through increased accumulation of glycosaminoglycan, an ECM component specific to cartilage, and upregulated expression of key chondrogenic genes (ACAN, SOX9, and Col2a1). Further, transplanted BMSCs loading MOF-HMME-RGD combined with SDT enhanced cartilage regeneration for cartilage defect repair after 8 weeks into treatment. This synergistic strategy based on MOF nanoparticles provides an instructive approach to developing alternative sonosensitizers for cartilage regeneration combined with SDT.
Decellularized biological matrices for the repair of rotator cuff lesions: a systematic review of preclinical in vivo studies
Authors: Codispoti Giorgia , Carniato Melania , Brogini Silvia , Romanelli Alessia , Martini Lucia , Giavaresi Gianluca , Tschon Matilde
Published: February 2024

Background: Rotator cuff tears (RCTs), resulting from degeneration or trauma of the shoulder tendons, are one of the main causes of shoulder pain. In particular, massive RCTs represent 40% of all injuries, require surgical treatment, and are characterized by poor clinical outcomes and a high rate of failure. In recent years, the use of biological decellularized patches for augmentation procedures has received great interest owing to their excellent self-integration properties, improving healing and, thus, presenting an innovative therapeutic option. However, the findings from clinical studies have emerged with conflicting viewpoints regarding the benefits of this procedure, as an excessive tension load might compromise the integrity of the tendon-to-bone connection when the patch exhibits low elasticity or insufficient strength. This could prevent the healing process, leading to unpredictable results in clinical practice.

Methods: This systematic review was conducted following Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines across three databases (PubMed, Scopus, and Web of Knowledge) to underline the results obtained in preclinical studies involving animal models of RCT surgeries that utilized the biological decellularized matrix augmentation technique in the last 5 years.

Results: Thirteen articles were included after the screening, and the SYRCLE tools were applied to assess the risk of bias in in vivo studies. Open-surgery techniques were conducted to create tendon defects or detachment in different animal models: rat (31%), rabbit (46%), dog (15%), and sheep (8%). Patches decellularized with non-standardized protocols were used in 77% of studies, while commercially available matrices were used in 15%. Of the studies, 31% used allogenic patches, 61% used xenogenic patches, and 8% utilized both xenogenic and autologous patches.

Conclusion: Overall, this review provides a comprehensive overview of the use of acellular patches and their effective therapeutic potential in rotator cuff (RC) repair at the preclinical level with the aim of expanding the strategies and matrices available for surgeons.

Systematic review registration:https://www.crd.york.ac.uk/prospero/, identifier CRD42023468716.
Solar governance for the transborder agglomeration of the Greater Geneva based on the solar cadaster development
Authors: Desthieux Gilles , Thebault Martin
Published: February 2024

As cities are major energy consumers challenges arise in densely populated areas that limit solar resources, hindering the deployment of urban solar power plants. To address this, the paper underscores the importance of digital tools for modeling solar energy accessibility within the urban fabric, focusing on rooftops, building facades, and other relevant locations. In this context, the development of the solar cadaster in Greater Geneva is presented in the paper within the framework of the European INTERREG G2 Solar project (2019–2022). The solar cadaster relies on a rich database from the Geneva Land Information System (SITG), offering comprehensive data on Digital Surface Models, building and roof cadasters, land cover, and more. Notably, this data, including the solar cadaster outputs, is accessible to the public as open data, fostering its extensive use in academia for teaching and research. It serves as a living laboratory for urban solar studies, with its open data approach contributing to widespread adoption. The project, a collaborative effort involving academia, energy utilities, and public institutions on both sides of the border, aims to unite stakeholders and foster a dialogue on solar energy in the region, in line with its commitment to addressing climate change, striving for carbon neutrality by 2050. The paper articulates the steps, methods, and outcomes of the project, offering insights into the challenges of implementing a shared online application tool in a cross-border territory. The propositions are based on feedbacks from stakeholders and lessons learned from case studies. The paper also delves into the shift in the business model for solar photovoltaic installations, emphasizing the need to model self-consumption in buildings. It concludes by highlighting the solar cadaster as a unifying means, fostering cohesion among diverse stakeholders in the region’s energy sector and propelling them toward common governance around solar energy.
GLT-1a glutamate transporter nanocluster localization is associated with astrocytic actin and neuronal Kv2 clusters at sites of neuron-astrocyte contact
Authors: Leek Ashley N. , Quinn Josiah A. , Krapf Diego , Tamkun Michael M.
Published: February 2024

Introduction: Astrocytic GLT-1 glutamate transporters ensure the fidelity of glutamic neurotransmission by spatially and temporally limiting glutamate signals. The ability to limit neuronal hyperactivity relies on the localization and diffusion of GLT-1 on the astrocytic surface, however, little is known about the underlying mechanisms. We show that two isoforms of GLT-1, GLT-1a and GLT-1b, form nanoclusters on the surface of transfected astrocytes and HEK-293 cells.

Methods: We used both fixed and live cell super-resolution imaging of fluorescent protein and epitope tagged proteins in co-cultures of rat astrocytes and neurons. Immunofluorescence techniques were also used. GLT1 diffusion was assessed via single particle tracking and fluorescence recovery after photobleach (FRAP).

Results: We found GLT-1a, but not GLT-1b, nanoclusters concentrated adjacent to actin filaments which was maintained after addition of glutamate. GLT-1a nanocluster concentration near actin filaments was prevented by expression of a cytosolic GLT-1a C-terminus, suggesting the C-terminus is involved in the localization adjacent to cortical actin. Using super-resolution imaging, we show that astrocytic GLT-1a and actin co-localize in net-like structures around neuronal Kv2.1 clusters at points of neuron/astrocyte contact.

Conclusion: Overall, these data describe a novel relationship between GLT-1a and cortical actin filaments, which localizes GLT-1a near neuronal structures responsive to ischemic insult.
Use of a Candida albicans SC5314 PacBio HiFi reads dataset to close gaps in the reference genome assembly, reveal a subtelomeric gene family, and produce accurate phased allelic sequences
Authors: Hoyer Lois L. , Freeman Brian A. , Hogan Elizabeth K. , Hernandez Alvaro G.
Published: February 2024

Candida albicans SC5314 is the most-often used strain for molecular manipulation of the species. The SC5314 reference genome sequence is the result of considerable effort from many scientists and has advanced research into fungal biology and pathogenesis. Although the resource is highly developed and presented in a phased diploid format, the sequence includes gaps and does not extend to the telomeres on its eight chromosome pairs. Accurate SC5314 genome assembly is complicated by the presence of extensive repeated sequences and considerable allelic length variation at some loci. Advances in genome sequencing technology provide the tools to obtain highly accurate long-read data that span even the most-difficult-to-assemble genome regions. Here, we describe derivation of a PacBio HiFi data set and creation of a collapsed haploid telomere-to-telomere assembly of the SC5314 genome (ASM3268872v1) that revealed previously unknown features of the strain. ASM3268872v1 subtelomeric distances were up to 19 kb larger than in the reference genome and revealed a family of highly conserved DNA helicase-encoding genes at 10 of the 16 chromosome ends. We also describe alignments of individual HiFi reads to deduce accurate diploid sequences for the most notoriously difficult-to-assemble C. albicans genes: the agglutinin-like sequence (ALS) gene family. We provide a tutorial that demonstrates how the HiFi reads can be visualized to explore any region of interest. Availability of the HiFi reads data set and the ASM3268872v1 comparative guide assembly will streamline research efforts because accurate diploid sequences can be derived using simple in silico methods rather than time-consuming laboratory-bench approaches.