bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi Field Coupled Coseismic Changes of the Jiashi Ms = 6.4 Earthquake of 19 January 2020, Based on Ground Temperature Observation
Authors: Jia Donghui , Zhao Binbin , Yu Huaizhong , Ma Yuchuan , Xiang Yuan , Yan Wei
Published: January 2022

The coseismic geothermal changes of ground temperature observed at observatories near the epicenter of the 2020 Jiashi Ms = 6.4 earthquake in China, provide a unique opportunity to study heat generation and conduction in rock. Here, evolutions of rock temperature at the Xikeer, Jiashizongchang, and Gedaliang observatories, which are located at epicentral distances of 1.4, 27.42, and 50 km respectively, were analyzed. Significant coseismic geothermal changes of 0.0432 °C were observed at the Xikeer observatory at the depth of 33.38 m, at which clear diurnal variations can be observed. Smaller changes of ~0.0001 °C were observed at the depths of 12.3 and 22.8 m at the Xikeer observatory and 22.3 m at the Jiashizongchang observatory. The stress transfer induced by the coseismic rupture induced a rise in local ground temperature, but the magnitude of the change was relatively small. The larger amplitude change at the Xikeer observatory was caused by fluid infiltration. We note that diurnal variation has been recorded at the Gedaliang observatory, but the coseismic response is no longer in existence. The temperature increases at the hypocentral area were higher than expected in the ground due to the coseismic stress transfer, but the change attenuated rapidly with distance.
Anti-Coagulant and Antimicrobial Recombinant Heparin-Binding Major Ampullate Spidroin 2 (MaSp2) Silk Protein
Authors: Mulinti Pranothi , Diekjürgen Dorina , Kurtzeborn Kristen , Balasubramanian Narayanaganesh , Stafslien Shane J. , Grainger David W. , Brooks Amanda E.
Published: January 2022

Governed by established structure–property relationships, peptide motifs comprising major ampullate spider silk confer a balance of strength and extensibility. Other biologically inspired small peptide motifs correlated to specific functionalities can be combined within these units to create designer silk materials with new hybrid properties. In this study, a small basic peptide, (ARKKAAKA) known to both bind heparin and mimic an antimicrobial peptide, was genetically linked to a protease-resistant, mechanically robust silk-like peptide, MaSp2. Purified fusion proteins (four silk domains and four heparin-binding peptide repeats) were expressed in E. coli. Successful fusion of a MaSp2 spider silk peptide with the heparin-binding motif was shown using a variety of analytical assays. The ability of the fusion peptide to bind heparin was assessed with ELISA and was further tested for its anticoagulant property using aPTT assay. Its intrinsic property to inhibit bacterial growth was evaluated using zone of inhibition and crystal violet (CV) assays. Using this strategy, we were able to link the two types of genetic motifs to create a designer silk-like protein with improved hemocompatibility and antimicrobial properties.
Serum Mature BDNF Level Is Associated with Remission Following ECT in Treatment-Resistant Depression
Authors: Psomiades Marion , Mondino Marine , Galvão Filipe , Mandairon Nathalie , Nourredine Mikail , Suaud-Chagny Marie-Françoise , Brunelin Jérôme
Published: January 2022

The search for a biological marker predicting the future failure or success of electroconvulsive therapy (ECT) remains highly challenging for patients with treatment-resistant depression. Evidence suggests that Brain-Derived Neurotrophic Factor (BDNF), a protein known to be involved in brain plasticity mechanisms, can play a key role in both the clinical efficacy of ECT and the pathophysiology of depressive disorders. We hypothesized that mature BDNF (mBDNF), an isoform of BDNF involved in the neural plasticity and survival of neural networks, might be a good candidate for predicting the efficacy of ECT. Total BDNF (tBDNF) and mBDNF levels were measured in 23 patients with severe treatment-resistant depression before (baseline) they received a course of ECT. More precisely, tBDNF and mBDNF measured before ECT were compared between patients who achieved the criteria of remission after the ECT course (remitters, n = 7) and those who did not (non-remitters, n = 16). We found that at baseline, future remitters displayed significantly higher mBDNF levels than future non-remitters (p = 0.04). No differences were observed regarding tBDNF levels at baseline. The multiple logistic regression model controlled for age and sex revealed that having a higher baseline mBDNF level was significantly associated with future remission after ECT sessions (odd ratio = 1.38; 95% confidence interval = 1.07–2.02, p = 0.04). Despite the limitations of the study, current findings provide additional elements regarding the major role of BDNF and especially the mBDNF isoform in the clinical response to ECT in major depression.
Early Palliative Care in Acute Myeloid Leukemia
Authors: Potenza Leonardo , Borelli Eleonora , Bigi Sarah , Giusti Davide , Longo Giuseppe , Odejide Oreofe , Porro Carlo Adolfo , Zimmermann Camilla , Efficace Fabio , Bruera Eduardo , Luppi Mario , Bandieri Elena
Published: January 2022

Background: Several novel targeted therapies seem to improve the outcome of acute myeloid leukemia (AML) patients. Nonetheless, the 5-year survival rate remains below 40%, and the trajectory of the disease remains physically and emotionally challenging, with little time to make relevant decisions. For patients with advanced solid tumors, the integration of early palliative care (EPC) with standard oncologic care a few weeks after diagnosis has demonstrated several benefits. However, this model is underutilized in patients with hematologic malignancies. Methods: In this article, we analyze the palliative care (PC) needs of AML patients, examine the operational aspects of an integrated model, and review the evidence in favor of EPC integration in the AML course. Results: AML patients have a high burden of physical and psychological symptoms and high use of avoidant coping strategies. Emerging studies, including a phase III randomized controlled trial, have reported that EPC is feasible for inpatients and outpatients, improves quality of life (QoL), promotes adaptive coping, reduces psychological symptoms, and enhances the quality of end-of-life care. Conclusions: EPC should become the new standard of care for AML patients. However, this raises issues about the urgent development of adequate programs of education to increase timely access to PC.
Prognostic and Clinicopathological Significance of the Aberrant Expression of β-Catenin in Oral Squamous Cell Carcinoma: A Systematic Review and Meta-Analysis
Authors: Ramos-García Pablo , González-Moles Miguel Á.
Published: January 2022

This systematic review and meta-analysis aims to evaluate the prognostic and clinicopathological significance of the aberrant expression of β-catenin (assessed through the immunohistochemical loss of membrane expression, cytoplasmic and nuclear expression) in oral squamous cell carcinoma (OSCC). We searched for primary-level studies published before October-2021 through PubMed, Embase, Web of Science, Scopus, and Google Scholar, with no limitation in regard to their publication date or language. We evaluated the methodological quality and risk of bias of the studies included using the QUIPS tool, carried out meta-analyses, explored heterogeneity and their sources across subgroups and meta-regression, and conducted sensitivity and small-study effects analyses. Forty-one studies (2746 patients) met inclusion criteria. The aberrant immunohistochemical expression of β-catenin was statistically associated with poor overall survival (HR = 1.77, 95% CI = 1.20–2.60, p = 0.004), disease-free survival (HR = 2.44, 95% CI = 1.10–5.50, p = 0.03), N+ status (OR = 2.39, 95% CI = 1.68–3.40, p < 0.001), higher clinical stage (OR = 2.40, 95% CI = 1.58–3.63, p < 0.001), higher tumour size (OR = 1.76, 95% CI = 1.23–2.53, p = 0.004), and moderately-poorly differentiated OSCC (OR = 1.57, 95% CI = 1.09–2.25, p = 0.02). The loss of β-catenin in the cell membrane showed the largest effect size in most of meta-analyses (singularly for poor overall survival [HR = 2.37, 95% CI = 1.55–3.62, p < 0.001], N+ status [OR = 3.44, 95% CI = 2.40–4.93, p < 0.001] and higher clinical stage [OR = 2.51, 95% CI = 1.17–5.35, p = 0.02]). In conclusion, our findings indicate that immunohistochemical assessment of the aberrant expression of β-catenin could be incorporated as an additional and complementary routine prognostic biomarker for the assessment of patients with OSCC.
Therapeutic Perspectives in the Systemic Treatment of Kaposi’s Sarcoma
Authors: Valantin Marc-Antoine , Royston Léna , Hentzien Maxime , Jary Aude , Makinson Alain , Veyri Marianne , Ronot-Bregigeon Sylvie , Isnard Stéphane , Palich Romain , Routy Jean-Pierre
Published: January 2022

In patients with Kaposi’s sarcoma (KS), the therapeutic goal is to achieve a durable remission in the size and number of skin and visceral lesions. Although most patients show tumor regression in response to standard systemic chemotherapy regimens, alternative systemic treatments are needed for patients who develop refractory KS. Anti-angiogenic therapies represent attractive therapeutic targets in this context, due to the central role of angiogenesis in KS pathogenesis. Pomalidomide, which exhibits such anti-angiogenic activity through inhibition of VEGF, currently constitutes the most promising agent of this class and has been recently approved by the FDA. In addition, immune checkpoint blockade also represents an interesting alternative therapeutic approach through the restoration of immunity against HHV-8, the causative agent of KS, and improvement of tumor control. Although small series of cases treated successfully with these drugs have been reported, there is no marketing approval for anti-immune checkpoint antibodies for KS to date. In the present review, we will discuss potential therapeutic options for patients with recurrent or refractory KS, including systemic chemotherapies, immune checkpoint inhibitors, anti-herpesvirus agents, and anti-angiogenic drugs. Well-conducted clinical trials in this population are urgently needed to correctly address the efficacy of targeted agents and immunomodulators, while monitoring for adverse effects.
Buffering Adaptive Immunity by Hydrogen Sulfide
Authors: Pozzi Giulia , Gobbi Giuliana , Masselli Elena , Carubbi Cecilia , Presta Valentina , Ambrosini Luca , Vitale Marco , Mirandola Prisco
Published: January 2022

T cell-mediated adaptive immunity is designed to respond to non-self antigens and pathogens through the activation and proliferation of various T cell populations. T helper 1 (Th1), Th2, Th17 and Treg cells finely orchestrate cellular responses through a plethora of paracrine and autocrine stimuli that include cytokines, autacoids, and hormones. Hydrogen sulfide (H2S) is one of these mediators able to induce/inhibit immunological responses, playing a role in inflammatory and autoimmune diseases, neurological disorders, asthma, acute pancreatitis, and sepsis. Both endogenous and exogenous H2S modulate numerous important cell signaling pathways. In monocytes, polymorphonuclear, and T cells H2S impacts on activation, survival, proliferation, polarization, adhesion pathways, and modulates cytokine production and sensitivity to chemokines. Here, we offer a comprehensive review on the role of H2S as a natural buffer able to maintain over time a functional balance between Th1, Th2, Th17 and Treg immunological responses.
Defective Cystinosin, Aberrant Autophagy−Endolysosome Pathways, and Storage Disease: Towards Assembling the Puzzle
Authors: Rega Laura Rita , De Leo Ester , Nieri Daniela , Luciani Alessandro
Published: January 2022

Epithelial cells that form the kidney proximal tubule (PT) rely on an intertwined ecosystem of vesicular membrane trafficking pathways to ensure the reabsorption of essential nutrients—a key requisite for homeostasis. The endolysosome stands at the crossroads of this sophisticated network, internalizing molecules through endocytosis, sorting receptors and nutrient transporters, maintaining cellular quality control via autophagy, and toggling the balance between PT differentiation and cell proliferation. Dysregulation of such endolysosome-guided trafficking pathways might thus lead to a generalized dysfunction of PT cells, often causing chronic kidney disease and life-threatening complications. In this review, we highlight the biological functions of endolysosome-residing proteins from the perspectives of understanding—and potentially reversing—the pathophysiology of rare inherited diseases affecting the kidney PT. Using cystinosis as a paradigm of endolysosome disease causing PT dysfunction, we discuss how the endolysosome governs the homeostasis of specialized epithelial cells. This review also provides a critical analysis of the molecular mechanisms through which defects in autophagy pathways can contribute to PT dysfunction, and proposes potential interventions for affected tissues. These insights might ultimately accelerate the discovery and development of new therapeutics, not only for cystinosis, but also for other currently intractable endolysosome-related diseases, eventually transforming our ability to regulate homeostasis and health.
Experiences and Attitudes on Early Identification Practices of Autism: A Preliminary Survey of Pediatric Professionals in Ecuador
Authors: Buffle Paulina , Naranjo Alfredo , Gentaz Edouard , Vivanti Giacomo
Published: January 2022

Early identification of children with autism is necessary to support their social and communicative skills and cognitive, verbal, and adaptive development. Researchers have identified several barriers to early diagnosis. Data collected in low- and middle-income contexts—where the vast majority of children in the world live—is scarce. In Ecuador, as in many other countries, estimates are lower compared to the global prevalence. Health authorities estimate a prevalence of 0.28% (0.18%–0.41%) in children aged five years old or less. Based on the hypothesis that, as in many parts of the world, children in Ecuador are not routinely screened and that this situation may result from poor recognition of this condition, our objective was to identify potential obstacles to case identification in pediatric settings. Several barriers, consistent with those identified in other countries, were reported by 153 participants on a survey, including lack of time, lack of resources to refer, fear of unnecessarily alarming families, and lack of information to guide families with concerns. The vast majority of participants are aware of the need for a screening tool for autism detection but report a lack of knowledge of its formal application. Most of the barriers reported in this study could be overcome by educational programs tailored to professional needs in order to support the well-being of children with autism and their families.
Diagnostic Efficacy of Rectal Suction Biopsy with Regard to Weight in Children Investigated for Hirschsprung’s Disease
Authors: Fransson Emma , Granéli Christina , Hagelsteen Kristine , Tofft Louise , Hambraeus Mette , Munoz Mitev Rodrigo Urdar , Gisselsson David , Stenström Pernilla
Published: January 2022

Background/aim: Diagnostic efficacy, defined as the percentage of rectal suction biopsy (RSB) specimens sufficient enough to determine the absence of ganglia cells in Hirschsprung’s disease (HD) diagnosis, has been reported to be low, requiring repeated biopsies. The aim was to explore whether RSB diagnostic efficacy was influenced by the child’s weight and to ascertain whether RSB efficacy differed between aganglionic and ganglionic tissue. Materials and Methods: Efficacy analyses were conducted in a national HD-center’s register on children 0–15 kg, examined between 2011–2019. First-time RSB diagnostic efficacy was correlated to the children’s weight and final HD diagnosis. Results: Among the 84 children who had first-time RSB, the overall diagnostic efficacy was 85% (71/84). The efficacy was higher among children weighing less than the identified cut-off of 9.0 kg (89% in 0–9.0 kg versus 62% in 9.01–15.0 kg, p = 0.026). Among children diagnosed with HD, 96% (26/27) weighed 0–9.0 kg. In this weight group, the diagnostic efficacy was lower in aganglionosis compared to ganglionosis (77%; 20/26 versus 96%; 43/45), p = 0.045). Conclusions: The RSB diagnostic efficacy was significantly higher in children weighing less than 9.0 kg and was less in aganglionic compared to ganglionic tissue. Therefore, weight can be useful to predict RSB diagnostic efficacy.
Ultra-Processed Foods Are the Major Sources of Total Fat, Saturated and Trans-Fatty Acids among Tunisian Preschool and School Children: A Cross-Sectional Study
Authors: Dogui Darine , Doggui Radhouene , Al-Jawaldeh Ayoub , El Ati Jalila , El Ati-Hellal Myriam
Published: January 2022

Excessive fat and fatty acids intake are associated with significant health hazards such as obesity or chronic diseases. This study aimed to provide the first data on total fat, saturated fatty acids (SFA) and trans fatty acids (TFA) intakes and their major food sources in Tunisian children. A total of 1200 children, aged 3 to 9 years old, were randomly selected from primary schools and kindergartens under a cross-sectional design. The 24-h dietary recall method and diet history for the month preceding the survey were used to assess dietary intake. The energy percentages of total fat, SFA and TFA in Tunisian children were 29.6%, 11.4% and 0.15%, respectively. No sex differences were found. The WHO recommendations for total fat, SFA and TFA were adopted by 58%, 39% and 89% of the study population, respectively. The leading food groups of fat and fatty acids were ultra-processed foods, breakfast cereals and dairy products. The meat, fish, eggs, and fish alternatives were the fifth main contributors to Tunisian children’s total fat and SFA intakes. The implementation of a relevant strategy for fat reduction, especially from ultra-processed foods, considered as low nutrient energy-dense products, is needed to promote health among children and prevent diet-related chronic diseases.
Long-Term Varicella Zoster Virus Immunity in Paediatric Liver Transplant Patients Can Be Achieved by Booster Vaccinations—A Single-Centre, Retrospective, Observational Analysis
Authors: Laue Tobias , Oms Elisabeth , Ohlendorf Johanna , Baumann Ulrich
Published: January 2022

Varicella is one of the most common vaccine-preventable infections after paediatric solid organ transplantation; thus, vaccination offers simple and cheap protection. However, children with liver disease often progress to liver transplantation (LT) before they reach the recommended vaccination age. As a live vaccine, varicella zoster virus (VZV) vaccination after transplantation is controversial; however, many case series demonstrate that vaccination may be safe and effective in paediatric liver transplant recipients. Only limited data exists describing long-term vaccination response in such immunocompromised patients. We investigated retrospectively vaccination response in paediatric patients before and after transplantation and describe long-term immunity over ten years, including the influence of booster-vaccinations. In this retrospective, single-centre study, 458 LT recipients were analysed between September 2004 and June 2021. Of these, 53 were re-transplantations. Patients with no available vaccination records and with a history of post-transplant lymphoproliferative disease, after hematopoietic stem cell transplantation and clinical chickenpox were excluded from this analysis (n = 198). In total, data on 207 children with a median annual follow-up of 6.2 years was available: 95 patients (45.9%) were unvaccinated prior to LT. Compared to healthy children, the response to vaccination, measured by seroconversion, is weaker in children with liver disease: almost 70% after one vaccination and 93% after two vaccinations. One year after transplantation, the mean titres and the number of children with protective antibody levels (VZV IgG ≥ 50 IU/L) decreased from 77.5% to 41.3%. Neither diagnosis, gender, nor age were predictors of vaccination response. Booster-vaccination was recommended for children after seroreversion using annual titre measurements and led to a significant increase in mean titre and number of protected children. Response to vaccination shows no difference from monotherapy with a calcineurin inhibitor to intensified immunosuppression by adding prednisolone or mycophenolate mofetil. Children with liver disease show weaker seroconversion rates to VZV vaccination compared to healthy children. Therefore, VZV-naïve children should receive basic immunization with two vaccine doses as well as those vaccinated only once before transplantation. An average of 2–3 vaccine doses are required in order to achieve a long-term seroconversion and protective antibody levels in 95% of children.
The Decline of Common Birds Exemplified by the Western Jackdaw Warns on Strong Environmental Degradation
Authors: Blanco Guillermo , Domínguez Luis , Fernández Luis , Martínez Félix , González del Barrio José L. , Frías Óscar , Cuevas Jesús A. , Carrete Martina
Published: January 2022

Bird populations associated with agricultural ecosystems have declined markedly in Europe during the last quarter of the 20th century due to land-use intensification. This has meant that some very common species, in some cases even species considered as pests, are now threatened or subject to management programs to ensure their conservation. Considered pests of crops and predators of small game species, corvids are among the most persecuted common farmland birds. The consideration that these birds are pests lacks any scientific evaluation and is justified by the subjective impression that they are abundant. Here, using estimates of absolute and relative abundances of both the total and the breeding population, we show how jackdaws Corvus monedula have shown a marked negative population trend in central Spain during the last 40 years. Decline involves the loss of multiple colonies, the apparent absence of the species as a breeder in riverside forests, and an overall numerical decrease of about 75% (from 35,000 to 9000 individuals) according to counts in communal roosts. The population decline seems to be more pronounced in areas where land use has been intensified, probably in response to the reduction in the availability of once-abundant food (i.e., invertebrates and weed seeds) but also due to more direct effects such as intoxication and medium to long-term accumulation of agricultural pollutants which may have also affected reproduction and survival. Intensive hunting over decades has undoubtedly contributed to this decline and should therefore be made forbidden urgently. Generally, it seems that high-intensity agricultural management more drastically affects smaller and less adaptable common species, which are expected to decline before and at a higher extent and magnitude than jackdaws. Given that global population estimates based on direct counts of individuals are readily achievable through simultaneous counts in communal roosts, the jackdaw can serve as a model for assessing temporal trends potentially linked to large-scale anthropogenic modifications of open and agricultural environments.
Never in Our Imaginations: The Public Human Resources Response to COVID-19 in Northwest Florida
Authors: Atkinson Christopher L.
Published: January 2022

The Coronavirus disease 2019 (COVID-19) pandemic has placed unprecedented demands on the whole of society. The public sector human resources (HR) function has played a key role in responding to COVID-19. This paper considers: How did public sector HR organizations in Northwest Florida respond during the COVID-19 pandemic? What are lessons learned from the perspectives of resilience and vulnerability? Interviews were conducted with HR professionals in Northwest Florida in early 2021. Responses suggested many points that show resilience, sensemaking, and adaptive capacity. However, some aspects of responses indicate the presence of vulnerability, as well as concerns with leadership and management.
Active Bionanocomposite Coating Quality Assessments of Some Cucumber Properties with Some Diverse Applications during Storage Condition by Chitosan, Nano Titanium Oxide Crystals, and Sodium Tripolyphosphate
Authors: Helal Mahmoud , Sami Rokayya , Algarni Eman , Alshehry Garsa , Aljumayi Huda , Al-Mushhin Amina A. M. , Benajiba Nada , Chavali Murthy , Kumar Nishant , Iqbal Abdullah , Aloufi Salman , Alyamani Amal , Madkhali Nawal , Almasoudi Abeer
Published: January 2022

Cucumbers have a short shelf-life of about 14 days, they are perishable due to the high moisture content. This study aimed to study the effects of nano-coating material such as titanium nanoparticles and chitosan with the addition of sodium tripolyphosphate as a crosslinker to enhance cucumber quality during storage. Some essential physical, chemical, and biological parameters were determined. CH-Nano-ST (chitosan/nano titanium oxide crystals/sodium tripolyphosphate) retained the maximum greenness, −7.99, compared to CH-Nano samples, which recorded −7.31. CH-Nano (chitosan/nano titanium oxide crystals) remained the lightest, 44.38, and CH-Nano-ST was a little darker (43.73) compared to the others treatments. The discoloration was extra severe with control (22.30), which started to spoil after the end of the first week. After 21 days of the storage period at 10 °C, the reducing sugars content reduced to reach −0.64 g/100 g and −0.21 g/100 g for CH-Nano and CH-Nano-ST treatments, respectively. The CH-Nano-ST treatment presented a lower value of toughness, followed by CH-Nano at the end of the storage period. Moreover, the highest crispness index was detected for CH-Nano (5.12%), while CH-Nano-ST treatment had a slight decline to reach 4.92%. The biological results indicated that CH-Nano-ST treatment can be applied to delay the microbial contamination of Salmonella spp. in cucumbers as it reached 0.94 log CFU/g, while the CH-Nano treatment reached 1.09 log CFU/g, at the end of the storage period. In summary, nano-coating treatments with the addition of sodium tripolyphosphate can be applied to regulator postharvest quality measurements of the biological activities in cucumbers during storage at 10 °C until 21 days.
Adaptive Stochastic Resonance-Based Processing of Weak Magnetic Slippage Signals of Bearings
Authors: Ma Jianpeng , Li Chengwei , Zhang Guangzhu
Published: January 2022

Slip is one of the most common forms of failure in aviation bearings, and it can pose a great threat to the stable operation of aviation bearings. Bearing cage speed monitoring methods based on weak magnetic detection can achieve nondestructive measurements. However, the method suffers from solid signal background noise due to the high sensitivity of the sensor. Therefore, in this paper, an adaptive stochastic resonance algorithm was proposed in response to the characteristics of the weak magnetic detection signal and the problem of solid noise. In addition, by adaptively adjusting the coefficients of the stochastic resonance system—by an improved moth flame optimization algorithm—the drawback in which the stochastic resonance method required artificially set parameters for extracting the feature frequencies of the weak magnetic signals was solved. In this process, we used parameters, such as general refined composite multi-scale sample entropy, as the adaptation function of the optimization algorithm. In the end, simulation and experimental outcomes verified the efficacy of the approach put forward.
No Effect of Early Adult Experience on the Development of Individual Specialization in Host-Searching Cabbage White Butterflies
Authors: Steck Meredith K. , Snell-Rood Emilie C.
Published: January 2022

Individuals in a population often use unique subsets of locally available resources, but we do not entirely understand how environmental context shapes the development of these specializations. In this study, we used ovipositing cabbage white butterflies (Pieris rapae) searching for host plants to test the hypothesis that early experience with an abundant resource can lead to later individual specialization. We first exposed naïve butterflies to one of three environments with different relative abundances of host plants of comparable nutritional quality, cabbage and radish. The next day, we observed butterflies from all treatments searching for hosts in a common environment where cabbage and radish were equally abundant. We predicted that the butterflies would preferentially visit the host plant that had been abundant during their previous experience, but instead found that butterflies from all experience treatments visited cabbage, a likely more visually salient host, more often than radish. In this experiment, behavioral plasticity in current conditions outweighed developmental experience in shaping individual resource use. We argue that these butterflies potentially respond to particularly salient search cues and that the discriminability of a resource may lead to specialization bias independent of early life experiences with abundant resources.
Importance of Protocol Design for Suitable Green In Situ Synthesis of ZnO on Cotton Using Aqueous Extract of Japanese Knotweed Leaves as Reducing Agent
Authors: Verbič Anja , Brenčič Katja , Primc Gregor , Gorjanc Marija
Published: January 2022

This work presents two protocols for the green in situ synthesis of zinc oxide nanoparticles (ZnO-NP) on cotton with the aim to develop sustainable cotton fabric with an ultraviolet protection factor (UPF). The protocols differed in the order of immersing cotton fabric in reactive solutions of three batches, i.e., precursor (0.1 M zinc acetate dihydrate), reducing agent (aqueous extract of Japanese knotweed leaves) and alkali (wood ash waste). The scanning electron microscope (SEM) results showed that ZnO-NP were successfully synthesised on cotton using both protocols; however, only the protocol where cotton was first immersed in alkali, then in the precursor and, lastly, in the reducing agent enabled very high UPF and higher amount of Zn present on the sample. Due to the different order of cotton fabric immersion in the reactive solutions, dissimilar morphology of the ZnO particles was observed, which resulted in different UV blocking abilities of the samples. The antioxidant analysis (DPPH) showed that the natural reducing agent prepared from Japanese knotweed leaves has very high antioxidant activity, which is attributed to phenolic compounds present in the plant. The reflectance spectroscopy results confirmed that the colour yield and colour of the samples did not influence the UPF value. This protocol is an example of green circular economy where waste materials of invasive alien plant species and pellet heating was used as a natural source of phytochemicals, for the direct synthesis of ZnO-NP to develop cotton fabric with UV-protective properties.
Evaluation of a Reputation Management Technique for Autonomous Vehicles
Authors: Kianersi Darius , Uppalapati Suraj , Bansal Anirudh , Straub Jeremy
Published: January 2022

Future autonomous vehicles will rely heavily on sharing and communicating information with other vehicles to maximize their efficiency. These interactions, which will likely include details about the positions of surrounding vehicles and obstacles on the road, are essential to their decision-making and the prevention of accidents. However, malicious vehicles—those that intentionally communicate false information—have the capacity to adversely influence other vehicles in the network. This paper presents and evaluates a reputation management system, capable of identifying malicious actors, to mitigate their effects on the vehicle network. The viability of multiple report weighting schemes to calculate reputation is evaluated through a simulation, and a blockchain-based backend for the reputation management system to securely maintain and communicate reputation data is proposed. Storage and computational challenges are considered. This paper shows that weighting schemas, related to the number and reputation of witnesses, positively affect the accuracy of the model and are able to identify malicious vehicles in a network with consistent accuracy and scalability.
Identification and Characterization of Differentially Expressed IgM Transcripts of Channel Catfish Vaccinated with Antigens of Virulent Aeromonas hydrophila
Authors: Zhang Dunhua , Lange Miles D. , Shoemaker Craig A. , Beck Benjamin H.
Published: January 2022

Channel catfish (Ictalurus punctatus) is the top species produced in US aquaculture and motile Aeromonas septicemia, caused by virulent Aeromonas hydrophila (vAh), is one of the most severe diseases that afflict catfish farms. Previously, vaccination of fish with extracellular proteins (ECP) of vAh was shown to produce a robust antibody-mediated immune response against vAh infection. In this study, we analyzed IgM transcripts that were differentially expressed in the head kidney and liver of ECP-immunized and mock-immunized (control) fish with emphasis on a variable domain of heavy chain. Quantitative PCR analysis indicated that immunized fish produced significantly more IgM transcripts than control fish. Full-length IgM heavy chain cDNA was cloned, which encoded typical IgM peptide, including signal peptide, variable domain (VH), constant domain (CH), and carboxyl terminal peptide. Great sequence diversity was revealed in a VH segment, with the third complementarity diversity region (CDR3) being most variable. Using germline VH gene grouping method, variants (clones) of VH characterized in this study belonged to nine VH families. The most unique variants (approximately 49%) were found in the VH2 family. Vaccinated fish apparently had more unique variants than in the control fish. There were 62% and 79% of unique variants in the head kidney and liver of vaccinated fish, respectively, while 44% and 27% unique variants in the head kidney and liver of control fish, respectively. Among the unique variants in VH2 family, approximately 87% of them were found in vaccinated fish. Two-dimensional gel electrophoresis of semi-purified IgM protein confirmed that matured IgM protein was as variable as IgM transcripts identified in this study, with isoelectric points crossing from 6 to 10. Results of this study provided insight into the molecular and genetic basis of antibody diversity and enriched our knowledge of the complex interplay between antigens and antibodies in Ictalurid catfish.
Detection of Single Burials Using Multispectral Drone Data: Three Case Studies
Authors: Rocke Benjamin , Ruffell Alastair
Published: January 2022

Natural burials are interments where a body is buried without embalming fluids or coffins. These burial grounds are ideal locations for retrospective multispectral analysis of non-conventional single burials as the age and location of each grave is documented. The detection of disturbed soil under the influence of human decomposition has been well-studied, but lacks the temporal component needed for characterising simulated clandestine burials. A critical gap in the literature is how these burials re-vegetate and to what extent soil profiles re-establish over years or decades. Multispectral drone data from three natural burial sites in southern U.K. are documented here, with trends in re-vegetation from bare soil to full recovery in graves as old as 2005. As with many burial detection techniques, environmental influence is a limiting variable to universal use of this method. However, we suggest a timeline over which single burial sites in this location reach detection limits and possible reasons for variations in these limits.
Nickel (II) and Cobalt (II) Alginate Biopolymers as a “Carry and Release” Platform for Polyhistidine-Tagged Proteins
Authors: Dumitrașcu Andrei-Mihai , Caraș Iuliana , Țucureanu Cătălin , Ermeneanu Andreea-Laura , Tofan Vlad-Constantin
Published: January 2022

Protein immobilization using biopolymer scaffolds generally involves undesired protein loss of function due to denaturation, steric hindrance or improper orientation. Moreover, most methods for protein immobilization require expensive reagents and laborious procedures. This work presents the synthesis and proof of concept application of two alginate hydrogels that are able to bind proteins with polyhistidine tags by means of interaction with the crosslinking cations. Nickel (II) and cobalt (II) alginate hydrogels were prepared using a simple ionic gelation method. Hydrogels were characterized by optical microscopy and AFM, and evaluated for potential cytotoxicity. In addition, binding capacity was tested towards proteins with or without HisTAG. Hydrogels had moderate cytotoxicity and were able to exclusively bind polyhistidine-tagged proteins with a binding capacity of approximately 300 µg EGFP (enhanced green fluorescent protein) per 1 mL of hydrogel. A lyophilized hydrogel-protein complex dissolved upon the addition of PBS and allowed the protein release and regain of biological activity. In conclusion, the nickel (II) and cobalt (II) alginate biopolymers provided an excellent platform for the “carry and release” of polyhistidine-tagged proteins.
Development of Loop-Mediated Isothermal Amplification Assay for Rapid Detection and Analysis of the Root-Knot Nematode Meloidogyne hapla in Soil
Authors: Omer Zahra Saad , Wallenhammar Ann-Charlotte , Viketoft Maria
Published: January 2022

Soil analysis is crucial for estimating the risk of crop damage by the root-knot nematode Meloidogyne hapla. Here, we developed an analysis assay based on Loop-mediated Isothermal Amplification (LAMP). The LAMP primers were verified for specificity against 10 different nematode species. A manual soil DNA extraction, referred to as SKMM, was developed and compared with a FastDNA kit followed by DNA purification. DNA was extracted with both methods from artificially inoculated soils as well as from naturally infested soil collected from farm fields. The primers exclusively amplified DNA from M. hapla with both colorimetric and real-time LAMP. The detection limit was 193 gene copies and 0.0016 juveniles (12 pg µL−1) per reaction. DNA concentrations and purity (A260/A230) were significantly higher using the SKMM procedure compared with the kit. From the field samples collected in 2019, DNA was amplified from 16% of samples extracted with SKMM and from 11% of samples using the kit. Occurrence of M. hapla DNA was confirmed in soil samples from two out of six field soils in 2020 using both real-time LAMP and qPCR. In conclusion, the developed real-time LAMP is a fast and specific assay for detection and quantification of M. hapla DNA in soil.
Learning Curve for Metastatic Liver Tumor Open Resection in Patients with Primary Colorectal Cancer: Use of the Cumulative Sum Method
Authors: Banas Bartlomiej , Gwizdak Piotr , Zabielska Paulina , Kolodziejczyk Piotr , Richter Piotr
Published: January 2022

Background: Liver resections have become the first-line treatment for primary and metastatic tumors and, therefore, are considered a core aspect of surgical training. This study aims to evaluate the learning curve of the extent and safety of liver resection procedures for patients with metastatic colorectal cancer. Methods: This single tertiary center retrospective analysis includes 158 consecutive cases of small liver resection (SLR) (n = 107) and major liver resection (MLR) (n = 58) procedures. A cumulative sum control chart (CUSUM) method was used to investigate the learning curve. Results: The operative time, total blood loss level, and incidence of adverse effects showed a learning curve. For SLRs, the CUSUM curve for operative time and blood loss level peaked at the 19th and 17th case, respectively, while for MLRs, these curves peaked at the 28th and 24th case, respectively. The CUSUM curve for minor adverse effects (MAEs) and severe adverse effects (SAEs) showed a downward slope after the 16th and 68th procedures in the SLRs group and after the 29th and 39th procedures in the MLRs cohort; however, it remained within the acceptable range throughout the entire study. Conclusion: SLR procedures were performed faster with less intraoperative blood loss and shorter postoperative stays than MLRs, and a higher number of completed procedures was required to gain stabilization and repeatability in the operating time and intraoperative blood loss level. In MLR procedures, the reduction of SAEs was accomplished significantly later than the stabilization of the operative time and intraoperative blood loss level.
Friends with Benefits: The Positive Consequences of Pet-Friendly Practices for Workers’ Well-Being
Authors: Junça-Silva Ana
Published: January 2022

Although there is evidence that pets may help individuals who are facing significant daily stressors, little is known about the benefits of pet-friendly practices for their owners’ well-being. Based on the social exchange theory and on the Rusbult investment model, we argue that organizational pet-friendly practices will be viewed as a source of support from an organization that increases workers’ organizational identification, which in turn will lead to higher levels of psychological well-being and life satisfaction. For this study, 208 working adults answered an online questionnaire. Results from the study showed that the more pet-friendly practices the higher the workers’ organizational identification, which led to higher indices of psychological well-being and life satisfaction. This study contributes to a better understanding of the human–animal interaction and how pets can function as a resource for individuals’ well-being at work.
Eight Good Reasons for Careful Monitoring and Evaluation of the Vaccine Campaign against COVID-19: Lessons Learned through the Lombardy Experience for Dealing with Next Challenges
Authors: Corrao Giovanni , Bertolaso Guido , Pavesi Giovanni , Moratti Letizia
Published: January 2022

Background: Using the knowledge gained during the first eleven months of the vaccine campaign in Lombardy, Italy, we provide an overview of the benefits of using reliable, complete, and rapidly available observational data to monitor the progress of the vaccine strategy. Methods: A population-based platform was implemented by linking four registries reporting individual data on: (i) date, type, and dose of vaccine dispensed; (ii) SARS-CoV-2 infections and hospital admissions and deaths due to COVID-19; (iii) inpatient diagnoses and outpatient services supplied by the Regional Health Services (RHS); and the (iv) health registry reporting and updating data on patient status. Background, methods, findings, and implications of eight COVID-19 relevant questions are reported. Results: Before starting the vaccine campaign, we identified high-risk individuals who need to be prioritized. During the vaccine campaign, we: (i) monitored the trend in the speed of the vaccine campaign progression and the number of prevented clinical outcomes; (ii) verified that available vaccines work in real-life, assessed their effectiveness-harm profile, and measured their reduced effectiveness against the delta variant. Finally, we studied the reduced effectiveness of the vaccine over time and identified risk factors of post-vaccine infection and severe illness. Conclusions: The correct use of rapidly available observational data of good quality and completeness generates reliable evidence to promptly inform patients and policymakers.
Direct Medical Cost of Stroke and the Cost-Effectiveness of Direct Oral Anticoagulants in Atrial Fibrillation-Related Stroke: A Cross-Sectional Study
Authors: Azahar Siti Norain , Sulong Saperi , Wan Zaidi Wan Asyraf , Muhammad Norliza , Kamisah Yusof , Masbah Norliana
Published: January 2022

Background: Stroke has significant direct medical costs, and direct oral anticoagulants (DOACs) are better alternatives to warfarin for stroke prevention in atrial fibrillation (AF). This study aimed to determine the direct medical costs of stroke, with emphasis on AF stroke and the cost-effectiveness of DOACs among stroke patients in a tertiary hospital in Malaysia. Methods: This study utilised in-patient data from the case mix unit of Universiti Kebangsaan Malaysia Medical Centre (UKMMC) between 2011 and 2018. Direct medical costs of stroke were determined using a top-down costing approach and factors associated with costs were identified. Incremental cost effectiveness ratio (ICER) was calculated to compare the cost-effectiveness between DOACs and warfarin. Results: The direct medical cost of stroke was MYR 11,669,414.83 (n = 3689). AF-related stroke cases had higher median cost of MYR 2839.73 (IQR 2269.79–3101.52). Regression analysis showed that stroke type (AF versus non-AF stroke) (p = 0.013), stroke severity (p = 0.010) and discharge status (p < 0.001) significantly influenced stroke costs. DOACs were cost-effective compared to warfarin with an ICER of MYR 19.25. Conclusions: The direct medical cost of stroke is substantial, with AF-stroke having a higher median cost per stroke care. DOACs were cost effective in the treatment of AF-related stroke in UKMMC.
Leisure Engagement during COVID-19 and Its Association with Mental Health and Wellbeing in U.S. Adults
Authors: Shen Xiangyou , MacDonald Megan , Logan Samuel W. , Parkinson Colby , Gorrell Lydia , Hatfield Bridget E.
Published: January 2022

Leisure engagement has risen as a salient societal issue during the COVID-19 pandemic, not only because it provides a pathway for people to continue meeting their physical, cognitive, and social-emotional needs, but also due to the phenomenal juxtaposition of general increases in leisure time and unparalleled constraints. This study reports the results of the first investigation of U.S. adults’ overall leisure engagement and its association with mental health amidst the major disruptions and sustained stress of the COVID-19 pandemic. Qualitative and quantitative data were collected through an online survey in February 2021 through Prolific from a sample representative of the U.S. adult population in age, gender, and race (n = 503) and analyzed using a mixed-method approach. A total of 104 unique leisure activities in 19 categories and 3 domains were identified through iterative thematic coding. Participants reported general increases in home-based traditional leisure and digital/online activities and decreases in physical and nature-based activities. Multiple regression analyses controlling for socio-demographic and context-specific covariates revealed distinct associations between changes in leisure engagement and different aspects of mental health (perceived stress, depressive symptoms, and mental wellbeing), supporting leisure’s dual role in facilitating stress alleviation and wellbeing enhancement during taxing events, such as COVID-19.