bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Supplementation with Black Soldier Fly Extract on Intestinal Function in Piglets Infected with Porcine Epidemic Diarrhea Virus
Authors: Yu Chenmin , Wu Mengjun , Sun Lanyuan , Li Hanxiao , Xu Zhaoyang , Zhang Qian , Yi Dan , Wang Lei , Zhao Di , Hou Yongqing , Wu Tao
Published: May 2024

Porcine epidemic diarrhea virus (PEDV) has developed as a global problem for the pig business, resulting in significant financial losses. Black soldier fly extract (BFE) has been proven to improve intestinal growth in pigs after weaning. Consequently, the goal of the present investigation was to explore the effects of BFE supplementation on intestinal function in PEDV-infected piglets. Eighteen piglets were randomly allocated to three groups: control, PEDV, and BFE + PEDV. The piglets in the BFE + PEDV group received 500 mg/kg BW of BFE orally for seven days from day 4 to 10 of the study. On day 9 of the study, six pigs from each group received either clean saline or PEDV solution at a dosage of 106 TCID50 (50% tissue culture infectious dose) per pig. On day 11, samples of blood and intestine were taken for additional investigation. The results indicated a significant decrease in the average daily gain (ADG) of piglets infected with PEDV (p < 0.05). Additionally, PEDV infection led to an alteration of blood indexes and a reduction in plasma D-xylose concentration and villi height in the small intestine, while it increased plasma diamine oxidase activity and small intestinal crypt depth in piglets (p < 0.05). The PEDV infection significantly reduced antioxidant enzyme activity in plasma and the gut, including total superoxide dismutase and catalase, while increasing contents of oxidation-relevant products such as malondialdehyde and hydrogen peroxide in piglets. Moreover, PEDV infection increased the mRNA expression level of antiviral-related genes (p < 0.05). Nutritional supplementation with BFE improved intestinal histomorphological indicators and reduced oxidative stress produced by PEDV infection in piglets. Interestingly, BFE could significantly promote the mRNA expression level of antiviral-related genes in the ileum (p < 0.05). Overall, the preliminary results suggest that dietary BFE could improve intestinal function in piglets after PEDV infection. Currently, the findings put a spotlight on the role of BFE in the prevention and treatment of PED in piglets.
Comparing the Prognostic Impacts of Delayed Administration of Appropriate Antimicrobials in Older Patients with Afebrile and Febrile Community-Onset Bacteremia
Authors: Hsueh Shu-Chun , Chen Po-Lin , Ho Ching-Yu , Hong Ming-Yuan , Lee Ching-Chi , Ko Wen-Chien
Published: May 2024

Although prompt administration of an appropriate antimicrobial therapy (AAT) is crucial for reducing mortality in the general population with community-onset bacteremia, the prognostic effects of delayed AAT in older individuals with febrile and afebrile bacteremia remain unclear. A stepwise and backward logistic regression analysis was used to identify independent predictors of 30-day mortality. In a 7-year multicenter cohort study involving 3424 older patients (≥65 years) with community-onset bacteremia, febrile bacteremia accounted for 27.1% (912 patients). A crucial association of afebrile bacteremia and 30-day mortality (adjusted hazard ratio [AHR], 1.69; p < 0.001) was revealed using Cox regression and Kaplan–Meier curves after adjusting for the independent predictors of mortality. Moreover, each hour of delayed AAT was associated with an average increase of 0.3% (adjusted odds ratio [AOR], 1.003; p < 0.001) and 0.2% (AOR, 1.002; p < 0.001) in the 30-day crude mortality rates among patients with afebrile and febrile bacteremia, respectively, after adjusting for the independent predictors of mortality. Similarly, further analysis based on Cox regression and Kaplan–Meier curves revealed that inappropriate empirical therapy (i.e., delayed AAT administration > 24 h) had a significant prognostic impact, with AHRs of 1.83 (p < 0.001) and 1.76 (p < 0.001) in afebrile and febrile patients, respectively, after adjusting for the independent predictors of mortality. In conclusion, among older individuals with community-onset bacteremia, the dissimilarity of the prognostic impacts of delayed AAT between afebrile and febrile presentation was evident.
Enhanced Efficacy of Ciprofloxacin and Tobramycin against Staphylococcus aureus When Combined with Corydalis Tuber and Berberine through Efflux Pump Inhibition
Authors: Seo Yena , Kim Minjun , Kim Tae-Jong
Published: May 2024

One way that bacteria develop antibiotic resistance is by reducing intracellular antibiotic concentrations through efflux pumps. Therefore, enhancing the efficacy of antibiotics using efflux pump inhibitors provides a way to overcome this type of resistance. Notably, an increasing number of pathogenic Staphylococcus aureus strains have efflux pump genes. In this study, the extract from Corydalis ternata Nakai tuber (Corydalis Tuber) at 512 mg/L was demonstrated to have an antibiotic synergistic effect with ciprofloxacin at 2 mg/L and tobramycin at 1024 mg/L against methicillin-resistant S. aureus (MRSA). Berberine, an isoquinoline alkaloid identified in Corydalis Tuber, was identified as contributing to this effect. Ethidium bromide efflux pump activity assays showed that Corydalis Tuber extract and berberine inhibited efflux, suggesting that they are efflux pump inhibitors. Molecular docking simulations suggested that berberine binds to S. aureus efflux pump proteins MepA, NorA, NorB, and SdrM. Additionally, berberine and Corydalis Tuber extract inhibit biofilm formation, which can confer antibiotic resistance. This study’s findings suggest that Corydalis Tuber, a traditional herbal medicine, and berberine, a medicinal supplement, act as S. aureus efflux pump inhibitors, synergistically increasing the efficacy of ciprofloxacin and tobramycin and showing promise as a treatment for antibiotic-resistant S. aureus infections, including MRSA.
Alcohol-Based Chlorhexidine and Potassium Sorbate Rub Strengthens the Effectiveness of Traditional Hand Scrubbing and Improves Long-Lasting Effectiveness—Evaluation of Hand Preparation Protocols According to EN 12791
Authors: Herráiz Soria Elena , Alou Luis , Martin-Villa Carlos , Becerro-de-Bengoa-Vallejo Ricardo , Losa-Iglesias Marta , Sevillano David
Published: May 2024

Despite the advantages of surgical handrub in terms of the ease of application and effectiveness, chlorhexidine (CHG)-based hand scrubbing remains the preferred method for surgical hand preparation. However, it does not systematically meet the non-inferiority requirement of the European norm (EN) 12791 with respect to n-propanol (the reference product) and does not provide the sustained efficacy expected for these long-lasting agents. Commercially available alcohol-based products have also failed to demonstrate sustained efficacy according to EN 12791. Multi-step protocols enhance the efficacy of hand scrubbing, yet their extended disinfection duration might diminish their allure for healthcare professionals. In this study, we show that hand scrubbing with CHG 4% followed by a 1 min rubbing with the novel formulation of ethanol (Et) 70%/CHG 3% plus 0.3% potassium sorbate food additive (PS) meets the non-inferiority requirement and demonstrates sustained efficacy when tested according to EN 12791. The immediate and 3 h effect of this protocol was significantly higher than that of n-propanol and the homologous disinfection protocol without PS (CHG 4% hand scrub plus Et 70%/CHG 3% rub), demonstrating that the inclusion of PS confers a notable residual effect. We speculate that this non-volatile ingredient acts synergistically with CHG. This promising combination represents an alternative method for the development of new disinfection strategies.
Hydration and Fortification of Common Bean (Phaseolus vulgaris L.) with Grape Skin Phenolics—Effects of Ultrasound Application and Heating
Authors: Bonassi Gloria , Lavelli Vera
Published: May 2024

Ultrasound (US)-assisted soaking combined with fortification with red grape skin (GS) phenolics was applied on two Phaseolus varieties, namely White Kidney Bean (WKB) and Cranberry Bean (CB), before heat treatment. The aims were to investigate: (a) the effect of US application on the kinetic of hydration; (b) the extent of absorption of different phenolic classes of GS into the beans and the resulting effect on antioxidant activity; (c) the effects of heat treatment on the phenolic fraction and antioxidant activity of GS extract- and water-soaked beans. US fastened the soaking step of both WKB and CB beans, which showed the sigmoidal and the downward concave shape hydration curves, respectively. Anthocyanins, flavonols, flavanol and phenolic acids levels increased with GS soaking, but US application was effective only for increasing the level of flavonols, while it favored the loss of endogenous phenolic acids and it did not affect the uptake of anthocyanins and flavanols. Heat treatment decreased the levels of most of phenolic compounds, but increased the levels of monomeric flavanols. Overall, the antioxidant activity was 40% higher in WKB and 53% higher in CB upon GS-fortification than in the control beans, despite the effects of heating. This fortification strategy could be applied for value addition of varieties low in phenolics or as a pre-treatment before intensive processing.
Lutein and Zeaxanthin Enhance, Whereas Oxidation, Fructosylation, and Low pH Damage High-Density Lipoprotein Biological Functionality
Authors: Zheng Jingyuan , Hong Brian V. , Agus Joanne K. , Tang Xinyu , Klebaner Nola R. , Chen Siyu , Guo Fei , Harvey Danielle J. , Lebrilla Carlito B. , Zivkovic Angela M.
Published: May 2024

High-density lipoproteins (HDLs) are key regulators of cellular cholesterol homeostasis but are functionally altered in many chronic diseases. The factors that cause HDL functional loss in chronic disease are not fully understood. It is also unknown what roles antioxidant carotenoids play in protecting HDL against functional loss. The aim of this study was to measure how various disease-associated chemical factors including exposure to (1) Cu2+ ions, (2) hypochlorous acid (HOCL), (3) hydrogen peroxide (H2O2), (4) sialidase, (5) glycosidase, (6) high glucose, (7) high fructose, and (8) acidic pH, and the carotenoid antioxidants (9) lutein and (10) zeaxanthin affect HDL functionality. We hypothesized that some of the modifications would have stronger impacts on HDL particle structure and function than others and that lutein and zeaxanthin would improve HDL function. HDL samples were isolated from generally healthy human plasma and incubated with the corresponding treatments listed above. Cholesterol efflux capacity (CEC), lecithin–cholesterol acyl transferase (LCAT) activity, and paraoxonase-1 (PON1) activity were measured in order to determine changes in HDL functionality. Median HDL particle diameter was increased by acidic pH treatment and reduced by HOCl, high glucose, high fructose, N-glycosidase, and lutein treatments. Acidic pH, oxidation, and fructosylation all reduced HDL CEC, whereas lutein, zeaxanthin, and sialidase treatment improved HDL CEC. LCAT activity was reduced by acidic pH, oxidation, high fructose treatments, and lutein. PON1 activity was reduced by sialidase, glycosidase, H2O2, and fructose and improved by zeaxanthin and lutein treatment. These results show that exposure to oxidizing agents, high fructose, and low pH directly impairs HDL functionality related to cholesterol efflux and particle maturation, whereas deglycosylation impairs HDL antioxidant capacity. On the other hand, the antioxidants lutein and zeaxanthin improve or preserve both HDL cholesterol efflux and antioxidant activity but have no effect on particle maturation.
Review of the Potential Role of Ascorbate in the Prevention and Treatment of Gynecological Cancers
Authors: Shen Xiaochang , Wang Jiandong , Deng Boer , Zhao Ziyi , Chen Shuning , Kong Weimin , Zhou Chunxiao , Bae-Jump Victoria
Published: May 2024

Ascorbate (vitamin C) is an essential vitamin for the human body and participates in various physiological processes as an important coenzyme and antioxidant. Furthermore, the role of ascorbate in the prevention and treatment of cancer including gynecological cancer has gained much more interest recently. The bioavailability and certain biological functions of ascorbate are distinct in males versus females due to differences in lean body mass, sex hormones, and lifestyle factors. Despite epidemiological evidence that ascorbate-rich foods and ascorbate plasma concentrations are inversely related to cancer risk, ascorbate has not demonstrated a significant protective effect in patients with gynecological cancers. Adequate ascorbate intake may have the potential to reduce the risk of human papillomavirus (HPV) infection and high-risk HPV persistence status. High-dose ascorbate exerts antitumor activity and synergizes with chemotherapeutic agents in preclinical cancer models of gynecological cancer. In this review, we provide evidence for the biological activity of ascorbate in females and discuss the potential role of ascorbate in the prevention and treatment of ovarian, endometrial, and cervical cancers.
Reaction Mechanisms of H2S Oxidation by Naphthoquinones
Authors: Olson Kenneth R. , Clear Kasey J. , Takata Tsuyoshi , Gao Yan , Ma Zhilin , Pfaff Ella , Travlos Anthony , Luu Jennifer , Wilson Katherine , Joseph Zachary , Kyle Ian , Kasko Stephen M. , Jones Jr Prentiss , Fukuto Jon , Xian Ming , Wu Gang , Straub Karl D.
Published: May 2024

1,4-naphthoquinones (NQs) catalytically oxidize H2S to per- and polysufides and sulfoxides, reduce oxygen to superoxide and hydrogen peroxide, and can form NQ-SH adducts through Michael addition. Here, we measured oxygen consumption and used sulfur-specific fluorophores, liquid chromatography tandem mass spectrometry (LC-MS/MS), and UV-Vis spectrometry to examine H2S oxidation by NQs with various substituent groups. In general, the order of H2S oxidization was DCNQ ~ juglone > 1,4-NQ > plumbagin >DMNQ ~ 2-MNQ > menadione, although this order varied somewhat depending on the experimental conditions. DMNQ does not form adducts with GSH or cysteine (Cys), yet it readily oxidizes H2S to polysulfides and sulfoxides. This suggests that H2S oxidation occurs at the carbonyl moiety and not at the quinoid 2 or 3 carbons, although the latter cannot be ruled out. We found little evidence from oxygen consumption studies or LC-MS/MS that NQs directly oxidize H2S2–4, and we propose that apparent reactions of NQs with inorganic polysulfides are due to H2S impurities in the polysulfides or an equilibrium between H2S and H2Sn. Collectively, NQ oxidation of H2S forms a variety of products that include hydropersulfides, hydropolysulfides, sulfenylpolysulfides, sulfite, and thiosulfate, and some of these reactions may proceed until an insoluble S8 colloid is formed.
Metabolomic Profiling of Floccularia luteovirens from Different Geographical Regions Proposes a Novel Perspective on Their Antioxidative Activities
Authors: Tang Chuyu , Fan Yuejun , Wang Tao , Wang Jie , Xiao Mengjun , He Min , Chang Xiyun , Li Yuling , Li Xiuzhang
Published: May 2024

Floccularia luteovirens, an endemic resource of the Tibetan Plateau, possesses significant medicinal and ecological values. However, the understanding of antioxidant capacity and metabolic profiling of F. luteovirens from diverse regions remains elusive due to limited resources. Therefore, to comprehensively comprehend the antioxidant capacity and metabolite diversity of F. luteovirens, we conducted a rounded analysis of its antioxidant capacity from three distinct regions using both untargeted and targeted metabolomics. Determination of antioxidant indices, such as ferric ion-reducing antioxidant power (FRAP), total phenolic content (TPC), and flavonoid content (FC), revealed the robust antioxidant capacity of F. luteovirens. QL F. luteovirens (QLFL) exhibited no significant difference compared to ZD F. luteovirens (ZDFL); however, both were significantly distinct from XH F. luteovirens (XHFL) across multiple indices. Furthermore, a positive correlation was observed between FRAP and flavonoid content. A total of 5782 metabolites were identified and chemically classified. Metabolites of F. luteovirens varied significantly at different regions and eight key differential metabolites were screened. Phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism, and cyanoamino acid metabolism were the main different regulatory pathways. Consequently, the disparities in the antioxidant activity of F. luteovirens may primarily be ascribed to the biosynthesis and metabolism of phenylalanine, while vanillic acid could potentially serve as a pivotal metabolite influencing the antioxidative capacity of F. luteovirens by targeted metabolomics. These findings enhance our understanding of the composition of F. luteovirens and provide valuable resources for its comprehensive utilization and targeted development.
Recent Advances in Robotics and Intelligent Robots Applications
Authors: Song Qi , Zhao Qinglei
Published: May 2024

Robotics research has a unique allure for both academia and the industry due to its potential for groundbreaking innovation and real-world applications [...]
Optimization of Ultrasound-Assisted Extraction of Free Phenolic Compounds and In Vitro Biological Activity from Peach Fruit Using Response Surface Methodology
Authors: Mihaylova Dasha , Terziyska Margarita , Desseva Ivelina , Popova Aneta , Lante Anna
Published: May 2024

In this study, the ultrasonic extraction (UAE) of free phenolic compounds and relative biological activities of the Bulgarian peach variety “Filina” was optimized using chemometric techniques (response surface methodology). A Box–Behnken design was used to reveal the variation in the hydro module, temperature, duration, and extractant on the total phenolic content, total flavonoid content, antioxidant potential, and inhibitory activity on each yield. The results revealed that the optimal conditions included a hydro module of 20, a duration of 39.33 min, a temperature of 70 °C, and an extractant of 96.64% to retrieve the highest level of bioactive compounds. The calculated parameters were discovered to be following the projected values.
Dual-Modality Cross-Interaction-Based Hybrid Full-Frame Video Stabilization
Authors: Jang Jaeyoung , Ban Yuseok , Lee Kyungjae
Published: May 2024

This study aims to generate visually useful imagery by preventing cropping while maintaining resolution and minimizing the degradation of stability and distortion to enhance the stability of a video for Augmented Reality applications. The focus is placed on conducting research that balances maintaining execution speed with performance improvements. By processing Inertial Measurement Unit (IMU) sensor data using the Versatile Quaternion-based Filter algorithm and optical flow, our research first applies motion compensation to frames of input video. To address cropping, PCA-flow-based video stabilization is then performed. Furthermore, to mitigate distortion occurring during the full-frame video creation process, neural rendering is applied, resulting in the output of stabilized frames. The anticipated effect of using an IMU sensor is the production of full-frame videos that maintain visual quality while increasing the stability of a video. Our technique contributes to correcting video shakes and has the advantage of generating visually useful imagery at low cost. Thus, we propose a novel hybrid full-frame video stabilization algorithm that produces full-frame videos after motion compensation with an IMU sensor. Evaluating our method against three metrics, the Stability score, Distortion value, and Cropping ratio, results indicated that stabilization was more effectively achieved with robustness to flow inaccuracy when effectively using an IMU sensor. In particular, among the evaluation outcomes, within the “Turn” category, our method exhibited an 18% enhancement in the Stability score and a 3% improvement in the Distortion value compared to the average results of previously proposed full-frame video stabilization-based methods, including PCA flow, neural rendering, and DIFRINT.
Enhanced High-Definition Video Transmission for Unmanned Driving in Mining Environments
Authors: Zhang Liya , Yang Wei , Li Chenxin
Published: May 2024

In the development of intelligent mines, unmanned driving transportation has emerged as a key technology to reduce human involvement and enable unmanned operations. The operation of unmanned vehicles in mining environments relies on remote operation, which necessitates the low-latency transmission of high-definition video data across multiple channels for comprehensive monitoring and precise remote control. To address the challenges associated with unmanned driving in mines, we propose a comprehensive scheme that leverages the capabilities of 5G super uplink, edge collaborative computing, and advanced video transmission strategies. This approach utilizes dual-frequency bands, specifically 3.5 GHz and 2.1 GHz, within the 5G super uplink framework to establish an infrastructure designed for high-bandwidth and low-latency information transmission, crucial for real-time autonomous operations. To overcome limitations due to computational resources at terminal devices, our scheme incorporates task offloading and edge computing methodologies to effectively reduce latency and enhance decision-making speed for real-time autonomous activities. Additionally, to consolidate the benefits of low latency, we implement several video transmission strategies, such as optimized network usage, service-specific wireless channel identification, and dynamic frame allocation. An experimental evaluation demonstrates that our approach achieves an uplink peak rate of 418.5 Mbps with an average latency of 18.3 ms during the parallel transmission of seven channels of 4K video, meeting the stringent requirements for remote control of unmanned mining vehicles.
Advanced 2D Computational Fluid Dynamics Model of an External Gear Pump Considering Relief Grooves
Authors: Nikolov Nikolay , Mitov Alexander , Kralov Ivan
Published: May 2024

The article presents an advanced two-dimensional (2D) computational fluid dynamics (CFD) model of an external gear pump which considers relief grooves. Relief grooves are limiting design features for the flow process of this type of pump, and their influence in existing studies is considered by a three-dimensional (3D) model only. The structural modification proposed by the authors is beyond the possibilities of real implementation, but it gives the possibility to precisely model the pump’s design features. In contrast to the existing studies (using 3D CFD), the proposed advanced 2D model requires significantly fewer computing resources. Numerical experiments were carried out using the 2D model at different pump operating modes depending on the rotation frequency (950–1450 min−1) and pressure load (5–150 bar). The numerical results were validated by a real-world experiment for the same pump operating modes using an existing laboratory experimental setup. An analysis of the CFD model and real experiment results was carried out by determining a quantitative index of match (FIT), which varies in the range of 97.93–99.82%. This proves the performance of the proposed CFD model, which can be further used as a part of more complex hydraulic systems models.
Multi-Step Design Optimization for the Improvement of an Outer-Rotor Brushless Direct Current Motor
Authors: Hsiao Chun-Yu , Htet Soe Min
Published: May 2024

Brushless Direct Current (BLDC) motors have seen significant improvements across various electrical applications. The growing focus on motor design research highlights the BLDC motor’s superior efficiency compared to traditional motors, which consume more power. BLDC motors are compact, lightweight, energy-efficient, and easy to control, making them ideal for modern applications. This study aims to enhance BLDC motor design and performance by employing the Taguchi method, Response Surface Methodology (RSM), and Finite Element Method (FEM) for multi-stage optimization. A 26-watt BLDC electric fan motor is the reference model for this study. The Taguchi method helps identify optimization points, guiding further enhancements in the second stage. The study proposes a design with improved output power, torque, and efficiency. The final design achieves a 15% higher energy efficiency than the reference model, with a 10 W increase in output power and a 0.032 Nm increase in maximum torque. The FEM analysis using JMAG software v 21.2 validates the proposed design, which shows improved configurations compared to the reference model, demonstrating the efficiency of the optimization techniques for BLDC motor design.
Study of Tunnel Vehicle GNSS/INS/OD Combination Position Based on Lateral Distance Measurement and Lane Line Constraint
Authors: Zhang Hongbin , Zhang Xu
Published: May 2024

The high-precision dynamic positioning of highway vehicles is the foundation and prerequisite for achieving intelligent connected transportation. To address the shortcomings of the GNSS/INS combination and GNSS/INS/OD combination in tunnel vehicle positioning, this paper proposes a tunnel vehicle positioning method for the GNSS/INS/OD combination based on lateral distance measurements and lane constraints. Firstly, a lateral distance measurement of vehicles inside the tunnel is conducted based on laser radar point cloud data. Secondly, map matching positioning is performed based on lateral distance measurements, odometer, and lane markings. Experimental results demonstrate that, for a 4.6 km tunnel, the average absolute error in the lateral positioning is 0.294 m, and the longitudinal positioning error is no more than 0.6 m, which can effectively meet practical operational requirements.
Unusual Animal Behavior as a Possible Candidate of Earthquake Prediction
Authors: Hayakawa Masashi , Yamauchi Hiroyuki
Published: May 2024

Short-term (with a lead time of about one week) earthquake (EQ) prediction is one of the most challenging subjects in geoscience and applied science; however, it is highly required by society because it is of essential importance in mitigating the human and economic losses associated with EQs. Electromagnetic precursors have recently been agreed to be the most powerful candidate for short-term prediction, because a lot of evidence has been accumulated on the presence of electromagnetic precursors (not only from the lithosphere, but also from the atmosphere and ionosphere) prior to EQs during the last three decades. On the other hand, unusual animal behavior associated with EQs, which is the main topic of this review, has been investigated as a macroscopic phenomenon for many years, with a much longer history than the study of seismo-electromagnetics. So, in this paper, we first summarize the previous research work on this general unusual animal behavior with reference to its relationship with EQs, and then we pay the greatest attention to our own previous work on dairy cows’ milk yield changes. We recommend this unusual animal behavior as an additional potential tool for short-term EQ prediction, which may be a supplement to the above seismo-electromagnetic effects. Finally, we will present our latest case study (as an example) on unusual changes of cows’ milk yields for a particular recent Tokyo EQ on 7 October 2021, and further propose that electromagnetic effects might be a possible sensory mechanism of unusual animal behavior, suggesting a close link between electromagnetic effects and unusual animal behavior.
How Does the Concentration of Technetium-99m Radiolabeled Gold Nanoparticles Affect Their In Vivo Biodistribution?
Authors: Apostolopoulou Adamantia , Salvanou Evangelia-Alexandra , Chiotellis Aristeidis , Pirmettis Nektarios N. , Pirmettis Ioannis C. , Xanthopoulos Stavros , Koźmiński Przemysław , Bouziotis Penelope
Published: May 2024

Gold nanoparticles (AuNPs) radiolabeled with therapeutic and diagnostic radioisotopes have been broadly studied as a promising platform for early diagnosis and treatment of many diseases including cancer. Our main goal for this study was the comparison of the biodistribution profiles of four different concentrations of gold nanoconjugates radiolabeled with Technetium-99m (99mTc). More specifically, AuNPs with an average diameter of 2 nm were functionalized with a tridentate thiol ligand. Four different concentrations were radiolabeled with 99mTc-tricarbonyls with high radiolabeling yields (>85%) and were further purified, leading to radiochemical purity of >95%. In vitro stability of the radiolabeled nanoconstructs was examined in cysteine and histidine solutions as well as in human serum, exhibiting robust radiolabeling up to 24 h post-preparation. Moreover, in vitro cytotoxicity studies were carried out in 4T1 murine mammary cancer cells. In vivo tracking of the radiolabeled nanoconjugates at both concentrations was examined in normal mice in order to examine the effect of AuNPs’ concentration on their in vivo kinetics. Our work demonstrates that varying concentrations of radiolabeled AuNPs lead to notably different biodistribution profiles.
Quantitative Detection for Fatigue Natural Crack in Aero-Aluminum Alloy Based on Pulsed Eddy Current Technique
Authors: Sun Cheng , Yu Yating , Li Hanchao , Wang Fenglong , Liu Dong
Published: May 2024

Aero-space aluminum alloys, as vital materials in aerospace engineering, find extensive application in various aerospace components. However, prolonged usage often leads to the emergence of fatigue natural cracks, posing significant safety risks. Therefore, research on accurate quantitative detection techniques for the cracks in aerospace-aluminum alloys is of vital importance. Firstly, based on the three-points bending experimental model, this paper prepared the fatigue natural crack specimen, and the depth of the natural crack is calibrated. Then, given the complexity of geometric characteristics inherent in natural cracks, the pulsed eddy current signal under the different natural crack depth is acquired and analyzed using an experimental study. Finally, to better exhibit the non-linearity between PEC signal and crack depth, a GA-based BPNN algorithm is proposed. The Latin Hypercube method is considered to optimize the population distribution in the genetic algorithm. The results indicate that the characterization accuracy reaches 2.19% for the natural crack.
Fermented Products Enriched with Polyunsaturated Fatty Acids in Broiler Chicken Nutrition and Fat Quality of Produced Meat
Authors: Makiš Andrej , Čertík Milan , Klempová Tatiana , Semjon Boris , Marcinčáková Dana , Jevinová Pavlína , Marcinčák Slavomír
Published: May 2024

Broiler chicken meat is the preferred meat among the human population. Broiler meat contains high-quality protein and a low-fat content, alongside a desirable fatty acid profile. A frequent problem in human nutrition is an insufficient PUFA intake in the diet. One possible strategy to increase the dietary intake of polyunsaturated fatty acids (PUFA) in humans is to produce, and thereby enrich, broiler chicken meat with sufficient amounts of essential PUFA. A method to increase the proportion of essential fatty acids in chicken meat is by changing the fatty acid composition of the feed. Feed production via solid-state fermentation using lower filamentous fungi can be used to prepare valuable feed from cereal by-products enriched with important PUFA and pigments and can thus be included as a suitable feed ingredient in the diet of chickens. From previously published studies, it can be concluded that the application of 3–10% of the prepared fermented products to the diet of broiler chickens increased the proportion of essential fatty acids in the fat of the chicken meat and had a beneficial effect on the growth parameters of chickens.
The Total Phenolic Content and Antioxidant Activity of Nine Monofloral Honey Types
Authors: Tananaki Chrysoula , Rodopoulou Maria-Anna , Dimou Maria , Kanelis Dimitrios , Liolios Vasilios
Published: May 2024

Honey is well known for its antioxidant and antimicrobial properties, which significantly contribute to its high demand among consumers. While there is plenty of information available about the antioxidant potential of honey, there is still a lack of research specifically focused on monofloral honeys, as most studies have been based on market samples. To address this issue, in the present study we analyzed the total phenolic content and antioxidant activity of nine monofloral honey types produced in Greece: fir, chestnut, citrus, erica, cotton, Jerusalem thorn, pine, oak and thyme, in comparison with manuka honey. The samples were collected from beekeepers applying the appropriate beekeeping practices. In total, ninety-six representative monofloral honey samples meeting the microscopic, physicochemical, and sensory characteristics were analyzed. Oak honey stood out as the darkest type (L* = 33.67) with the highest total phenolic content (203.75 mg GAE/100 g) and antioxidant activity (106.2 mg AAE/100 g). Chestnut honey closely followed, having also the highest electrical conductivity (1.679 mS/cm). Although manuka honey had a high total phenolic content, its total antioxidant activity was found to be medium-low compared to fir, pine, and erica honeys. Citrus honey, being the lightest in color (L* = 37.2), exhibited the lowest total antioxidant activity (6.36 mg AAE/100 g). Statistical analysis revealed significant positive correlation between total antioxidant activity and electrical conductivity (ra-e = 0.587, pa-e = 0.000), and negative correlation between total antioxidant activity and L* parameter (ra-L = −0.424, pa-L = 0.000). Similar correlations were also observed regarding total phenolic content (rp-e = 0.457, pp-e = 0.000, rp-L = −0.455, pp-L = 0.000). In conclusion, oak and chestnut honeys seem to have a high antioxidant potential, that should be further explored, to highlight their value and help promote them worldwide.
Sustainable Management of Rechargeable Batteries Used in Electric Vehicles
Authors: Meegoda Jay , Charbel Ghadi , Watts Daniel
Published: May 2024

A Life Cycle Assessment (LCA) quantifies the environmental impacts during the life of a product from cradle to grave. It evaluates energy use, material flow, and emissions at each stage of life. This report addresses the challenges and potential solutions related to the surge in electric vehicle (EV) batteries in the United States amidst the EV market’s exponential growth. It focuses on the environmental and economic implications of disposal as well as the recycling of lithium-ion batteries (LIBs). With millions of EVs sold in the past decade, this research highlights the necessity of efficient recycling methods to mitigate environmental damage from battery production and disposal. Utilizing a Life Cycle Assessment (LCA) and Life Cycle Cost Assessment (LCCA), this research compares emissions and costs between new and recycled batteries by employing software tools such as SimaPro V7 and GREET V2. The findings indicate that recycling batteries produces a significantly lower environmental impact than manufacturing new units from new materials and is economically viable as well. This research also emphasizes the importance of preparing for the upcoming influx of used EV batteries and provides suggestions for future research to optimize the disposal and recycling of EV batteries.
Study of a Deep Convolution Network with Enhanced Region Proposal Network in the Detection of Cancerous Lung Tumors
Authors: Lee Jiann-Der , Hsu Yu-Tsung , Chien Jong-Chih
Published: May 2024

A deep convolution network that expands on the architecture of the faster R-CNN network is proposed. The expansion includes adapting unsupervised classification with multiple backbone networks to improve the Region Proposal Network in order to improve accuracy and sensitivity in detecting minute changes in images. The efficiency of the proposed architecture is investigated by applying it to the detection of cancerous lung tumors in CT (computed tomography) images. This investigation used a total of 888 images from the LUNA16 dataset, which contains CT images of both cancerous and non-cancerous tumors of various sizes. These images are divided into 80% and 20%, which are used for training and testing, respectively. The result of the investigation through the experiment is that the proposed deep-learning architecture could achieve an accuracy rate of 95.32%, a precision rate of 94.63%, a specificity of 94.84%, and a high sensitivity of 96.23% using the LUNA16 images. The result shows an improvement compared to a reported accuracy of 93.6% from a previous study using the same dataset.
Cortical and Trabecular Bone Modeling and Implications for Bone Functional Adaptation in the Mammalian Tibia
Authors: Barak Meir M.
Published: May 2024

Bone modeling involves the addition of bone material through osteoblast-mediated deposition or the removal of bone material via osteoclast-mediated resorption in response to perceived changes in loads by osteocytes. This process is characterized by the independent occurrence of deposition and resorption, which can take place simultaneously at different locations within the bone due to variations in stress levels across its different regions. The principle of bone functional adaptation states that cortical and trabecular bone tissues will respond to mechanical stimuli by adjusting (i.e., bone modeling) their morphology and architecture to mechanically improve their mechanical function in line with the habitual in vivo loading direction. This principle is relevant to various research areas, such as the development of improved orthopedic implants, preventative medicine for osteopenic elderly patients, and the investigation of locomotion behavior in extinct species. In the present review, the mammalian tibia is used as an example to explore cortical and trabecular bone modeling and to examine its implications for the functional adaptation of bones. Following a short introduction and an exposition on characteristics of mechanical stimuli that influence bone modeling, a detailed critical appraisal of the literature on cortical and trabecular bone modeling and bone functional adaptation is given. By synthesizing key findings from studies involving small mammals (rodents), large mammals, and humans, it is shown that examining both cortical and trabecular bone structures is essential for understanding bone functional adaptation. A combined approach can provide a more comprehensive understanding of this significant physiological phenomenon, as each structure contributes uniquely to the phenomenon.
The Association of Death Receptors and TGF-β1 Expression in Urothelial Bladder Cancer and Their Prognostic Significance
Authors: Stojnev Slavica , Conic Irena , Ristic Petrovic Ana , Petkovic Ivan , Radic Milica , Krstic Miljan , Jankovic Velickovic Ljubinka
Published: May 2024

Death receptor signalization that triggers the extrinsic apoptotic pathway and TGF-β1 have important roles in urothelial carcinogenesis, with a complex interplay between them. The aim of this research was to assess the association of death receptors DR4, DR5, and FAS as well as TGF-β1 immunohistochemical expression with the clinicopathological characteristics of urothelial bladder cancer (UBC) and to evaluate their prognostic significance. The decrease or loss of death receptors’ expression was significantly associated with muscle-invasive tumors, while non-invasive UBC often retains the expression of death receptors, which are mutually strongly linked. High DR4 expression is a marker of low-grade tumors and UBC associated with exposition to known carcinogens. Conversely, TGF-β1 was significantly associated with high tumor grade and advanced stage. High expression of DR4 and FAS indicates longer overall survival. High TGF-β1 signifies an inferior outcome and is an independent predictor of adverse prognosis in UBC patients. This study reveals the expression profile of death receptors in UBC and their possible interconnection with TGF-β1 and indicates independent prognostic significance of high FAS and TGF-β1 expression in UBC, which may contribute to deciphering the enigma of UBC heterogeneity in light of the rapid development of novel and effective therapeutic approaches, including targeting of the TRAIL-induced apoptotic pathway.
Insights into Kidney Dysplasia in Duplex Kidneys: From Radiologic Diagnosis to Histopathologic Understanding
Authors: Świętoń Dominik , Buczkowski Kamil , Czarniak Piotr , Gołębiewski Andrzej , Grzywińska Małgorzata , Kujawa Mariusz J. , Back Susan J. , Piskunowicz Maciej , Iżycka-Świeszewska Ewa
Published: May 2024

Duplex kidney is a urinary tract anomaly commonly associated with a wide range of primary and secondary parenchymal structural abnormalities. We present a unique comparison of US and MRI findings with histopathology following partial resection of duplex kidneys due to nephropathy. We examined a group of 21 children with duplex kidneys who were qualified for heminephrectomy (24 kidney units (KU)). All patients underwent US and MRI prior to the surgery. The imaging results were compared with histopathologic findings. In 21/24 KU, dysplastic changes were found on histopathology, including all with obstructive nephropathy and 7/10 specimens with refluxing uropathy. The loss of corticomedullary differentiation on US and increased signal on T2-weighted images (T2WI) on MRI were the imaging findings that best correlated with fibrosis. In children with megaureter, there were no statistical differences in histopathological findings between primary megaureter, megaureter with ureterocele, and megaureter with ectopia (p > 0.05). The extent of dysplasia of the affected pole correlated negatively with residual function in MRI. Kidney dysplasia and inflammation in the kidney with obstructive nephropathy are the most important histopathologic findings of this study. US is a valuable screening tool, and MRI enables morphologic and functional assessments of the nephropathy in duplex kidneys.
Characterizing the Dynamic Expression of C1q/TNF-α-Related Protein 6 (CTRP6) during Pregnancy in Humans and Mice with Gestational Diabetes Mellitus
Authors: Jiang Jianan , Wei Shuangyu , Chen Miao , Tan Yutian , Yang Zhao , Yang Guiying , Feng Weijie , Han Zhen , Wei Xiaojing , Luo Xiao
Published: May 2024

Aim: C1q/TNF-related protein 6 (CTRP6) is a novel adipokine involved in insulin resistance. Thus, we aim to investigate the expression profile of CTRP6 in the plasma, adipose tissue and placenta of GDM patients and mice. Methods: Chinese Han pregnant women (GDM n = 9, control n = 10) with a scheduled caesarean section delivery were recruited. A number of high-fat diet (HFD) induced-pregnancy C57BL/6 mice were chosen as an animal model of GDM. Circulating levels of CTRP6 and adiponectin were examined by ELISA. CTRP6 expression in adipose tissue and placenta were detected by real-time qPCR and WB. Result: Plasma CTRP6 levels were decreased during the first and second trimesters in mice, as well as the second and third trimesters in patients, while they were increased at delivery in GDM patients and mice. Plasma CTRP6 levels were significantly correlated with WBC, systolic pressure, diastolic pressure and fasting blood glucose. Moreover, CTRP6 mRNA expression in the subcutaneous (sWAT) and omental white adipose tissue (oWAT), as well as in the placenta, was significantly higher in GDM human patients at cesarean delivery. Furthermore, the mRNA expression of Ctrp6 was increased in the sWAT and visceral WAT (vWAT), whilst decreased in the interscapular brown adipose tissue (iBAT), of GDM mice at cesarean delivery. Conclusion: Dynamically expressed CTRP6 may be served as a candidate target for treatment of GDM.
Regulation of the Function and Expression of EpCAM
Authors: Xiao Di , Xiong Mingrui , Wang Xin , Lyu Mengqing , Sun Hanxiang , Cui Yeting , Chen Chen , Jiang Ziyu , Sun Fan
Published: May 2024

The epithelial cell adhesion molecule (EpCAM) is a single transmembrane protein on the cell surface. Given its strong expression on epithelial cells and epithelial cell-derived tumors, EpCAM has been identified as a biomarker for circulating tumor cells (CTCs) and exosomes and a target for cancer therapy. As a cell adhesion molecule, EpCAM has a crystal structure that indicates that it forms a cis-dimer first and then probably a trans-tetramer to mediate intercellular adhesion. Through regulated intramembrane proteolysis (RIP), EpCAM and its proteolytic fragments are also able to regulate multiple signaling pathways, Wnt signaling in particular. Although great progress has been made, increasingly more findings have revealed the context-specific expression and function patterns of EpCAM and their regulation processes, which necessitates further studies to determine the structure, function, and expression of EpCAM under both physiological and pathological conditions, broadening its application in basic and translational cancer research.
Modeling the Electrical Activity of the Heart via Transfer Functions and Genetic Algorithms
Authors: Rodríguez-Abreo Omar , Cruz-Fernandez Mayra , Fuentes-Silva Carlos , Quiroz-Juárez Mario A. , Aragón José L.
Published: May 2024

Although healthcare and medical technology have advanced significantly over the past few decades, heart disease continues to be a major cause of mortality globally. Electrocardiography (ECG) is one of the most widely used tools for the detection of heart diseases. This study presents a mathematical model based on transfer functions that allows for the exploration and optimization of heart dynamics in Laplace space using a genetic algorithm (GA). The transfer function parameters were fine-tuned using the GA, with clinical ECG records serving as reference signals. The proposed model, which is based on polynomials and delays, approximates a real ECG with a root-mean-square error of 4.7% and an R2 value of 0.72. The model achieves the periodic nature of an ECG signal by using a single periodic impulse input. Its simplicity makes it possible to adjust waveform parameters with a predetermined understanding of their effects, which can be used to generate both arrhythmic patterns and healthy signals. This is a notable advantage over other models that are burdened by a large number of differential equations and many parameters.
Biomechanical Multipurpose Miniscrew Strategy for Simultaneous Distalization in Class II Patients—The BiGa System
Authors: Di Carlo Gabriele , Biondi Guglielmo , Gazzola Ivan , Saccucci Matteo
Published: May 2024

An efficient treatment plan using a temporary anchorage device should be built following the principle of reducing the number of tads to obtain a multiple biomechanical advantage. The following case report concerns the Biga system, a strategy that supports orthodontists during class II corrections and vertical control through treatment. A 12-year-old girl with a high angle of skeletal class II was selected. A novel biomechanical strategy was effectively applied using two tads on the upper arch to obtain sequential distalization of the upper teeth and to correct the lower arch spee curve using third-class elastics. Eventually, on the same tads, a double cantilever was applied to control the overbite and intrusion during incisors’ retraction. The Biga system is an easy biomechanical strategy that ensures the three-dimensional control of treatment mechanics in class II patients.