bluuebunny/crossref_metadata_2025 huggingface dataset:
Mutational inactivation of mTORC1 repressor gene
DEPDC5
in human gastrointestinal stromal tumors
Authors: Pang Yuzhi , Xie Feifei , Cao Hui , Wang Chunmeng , Zhu Meijun , Liu Xiaoxiao , Lu Xiaojing , Huang Tao , Shen Yanying , Li Ke , Jia Xiaona , Li Zhang , Zheng Xufen , Wang Simin , He Yi , Wang Linhui , Fletcher Jonathan A. , Wang Yuexiang
Published: October 2019

Gastrointestinal stromal tumors (GISTs) are the most common human sarcoma and are initiated by activating mutations in the
KIT
or
PDGFRA
receptor tyrosine kinases. Chromosome 22q deletions are well-recognized frequent abnormalities in GISTs, occurring in ∼50% of GISTs. These deletions are thought to contribute to the pathogenesis of this disease via currently unidentified tumor suppressor mechanisms. Using whole exome sequencing, we report recurrent genomic inactivated
DEPDC5
gene mutations in GISTs (16.4%, 9 of 55 patients). The demonstration of clonal
DEPDC5
inactivation mutations in longitudinal specimens and in multiple metastases from individual patients suggests that these mutations have tumorigenic roles in GIST progression. DEPDC5 inactivation promotes GIST tumor growth in vitro and in nude mice. DEPDC5 reduces cell proliferation through the mTORC1-signaling pathway and subsequently induces cell-cycle arrest. Furthermore, DEPDC5 modulates the sensitivity of GIST to KIT inhibitors, and the combination therapy with mTOR inhibitor and KIT inhibitor may work better in GIST patients with DEPDC5 inactivation. These findings of recurrent genomic alterations, together with functional data, validate the DEPDC5 as a bona fide tumor suppressor contributing to GIST progression and a biologically relevant target of the frequent chromosome 22q deletions.
Aged hind-limb clasping experimental autoimmune encephalomyelitis models aspects of the neurodegenerative process seen in multiple sclerosis
Authors: Cahill Lindsay S. , Zhang Monan Angela , Ramaglia Valeria , Whetstone Heather , Sabbagh Melika Pahlevan , Yi Tae Joon , Woo Laura , Przybycien Thomas S. , Moshkova Marina , Zhao Fei Linda , Rojas Olga L. , Gomes Josephine , Kuerten Stefanie , Gommerman Jennifer L. , Sled John G. , Dunn Shannon E.
Published: October 2019

Significance

EAE typically presents with hind-limb paralysis and is associated with severe T cell-mediated inflammation and axon injury throughout the spinal cord and parts of the brain. Because axon loss is so rapid in this model, it is difficult to intervene for the purpose of evaluating neuroprotective therapies. Here we describe a mild form of EAE that does not even meet the threshold for scoring on traditional EAE scales, yet with age results in devastating neurodegenerative changes in the spinal cord, including grey matter atrophy, neuron loss, and synapse degradation. These observations underscore the utility of T cell receptor transgenic mice to study the effect of natural aging on CNS autoimmunity and neurodegeneration.
Graded regulation of cellular quiescence depth between proliferation and senescence by a lysosomal dimmer switch
Authors: Fujimaki Kotaro , Li Ruoyan , Chen Hengyu , Della Croce Kimiko , Zhang Hao Helen , Xing Jianhua , Bai Fan , Yao Guang
Published: October 2019

The reactivation of quiescent cells to proliferate is fundamental to tissue repair and homeostasis in the body. Often referred to as the G0 state, quiescence is, however, not a uniform state but with graded depth. Shallow quiescent cells exhibit a higher tendency to revert to proliferation than deep quiescent cells, while deep quiescent cells are still fully reversible under physiological conditions, distinct from senescent cells. Cellular mechanisms underlying the control of quiescence depth and the connection between quiescence and senescence are poorly characterized, representing a missing link in our understanding of tissue homeostasis and regeneration. Here we measured transcriptome changes as rat embryonic fibroblasts moved from shallow to deep quiescence over time in the absence of growth signals. We found that lysosomal gene expression was significantly up-regulated in deep quiescence, and partially compensated for gradually reduced autophagy flux. Reducing lysosomal function drove cells progressively deeper into quiescence and eventually into a senescence-like irreversibly arrested state; increasing lysosomal function, by lowering oxidative stress, progressively pushed cells into shallower quiescence. That is, lysosomal function modulates graded quiescence depth between proliferation and senescence as a dimmer switch. Finally, we found that a gene-expression signature developed by comparing deep and shallow quiescence in fibroblasts can correctly classify a wide array of senescent and aging cell types in vitro and in vivo, suggesting that while quiescence is generally considered to protect cells from irreversible arrest of senescence, quiescence deepening likely represents a common transition path from cell proliferation to senescence, related to aging.
English language assistants in the 21st century
Authors: Codó Eva , McDaid Jessica
Published: October 2019

Although the figure of the English language assistant (ELA) dates back a long while, its current popularity is unprecedented in some areas of the world. Such is the case of Spain, where the goal of raising English standards among the younger generations has become a national obsession. Using critical ethnographic methods, this paper examines the experience of three British LAs placed in secondary schools in Barcelona. It draws on a focused case study of one of them – combined with ethnographic snapshots of the other two, interviews with school teachers and regional programme administrators, relevant programme publications, and social media data. The analysis reveals three major tensions shaping the ELA experience in the 21st century revolving around: (a) the underspecified and unskilled nature of the job; (b) its culturalist imagination and state diplomacy mission; and (c) the native speaker ideology constituting itsraison d’être. This paper provides new insights into the intertwining of the ELT infrastructure with global travel and tourism capitalised as skill boosters for employability purposes, and showcases the importance of foreign language education as a soft power tool.
Par-1 controls the composition and growth of cortical actin caps during Drosophila embryo cleavage
Authors: Jiang Tao , Harris Tony J.C.
Published: October 2019

Cell structure depends on the cortex, a thin network of actin polymers and additional proteins underlying the plasma membrane. The cell polarity kinase Par-1 is required for cells to form following syncytial Drosophila embryo development. This requirement stems from Par-1 promoting cortical actin caps that grow into dome-like metaphase compartments for dividing syncytial nuclei. We find the actin caps to be a composite material of Diaphanous (Dia)-based actin bundles interspersed with independently formed, Arp2/3-based actin puncta. Par-1 and Dia colocalize along extended regions of the bundles, and both are required for the bundles and for each other’s bundle-like localization, consistent with an actin-dependent self-reinforcement mechanism. Par-1 helps establish or maintain these bundles in a cortical domain with relatively low levels of the canonical formin activator Rho1-GTP. Arp2/3 is required for displacing the bundles away from each other and toward the cap circumference, suggesting interactions between these cytoskeletal components could contribute to the growth of the cap into a metaphase compartment.
Brain–heart interactions considering complex physiological data: processing schemes for time-variant, frequency-dependent, topographical and statistical examination of directed interactions by convergent cross mapping
Authors: Schiecke Karin , Schumann Andy , Benninger Franz , Feucht Martha , Baer Karl-Juergen , Schlattmann Peter
Published: October 2019

Background: A multitude of complex methods is available to quantify interactions in highly complex physiological systems. Brain–heart interactions play an important role in identifying couplings between the central nervous system and the autonomic nervous system during defined physiological states or specific diseases. The crucial point of those interaction analyses is adequate pre-processing taking into account nonlinearity of data, intuitive graphical representation and suitable statistical evaluation of the achieved results. Objective: The aim of this study is to provide generalized processing schemes for such investigations taking into account pre-processing, graphical representation and statistical analysis. Approach: Two defined data sets were used to develop these processing schemes. Brain–heart interactions in children with temporal lobe epilepsy during the pre-ictal, ictal and post-ictal periods as well as in patients with paranoid schizophrenia and healthy control subjects during the resting state period were investigated by nonlinear convergent cross mapping (CCM). Surrogate data, bootstrapping and linear mixed-effects model approaches were utilized for statistical analyses. Main results: CCM was able to reveal specific and statistically significant time- and frequency-dependent patterns of brain–heart interactions for children with temporal lobe epilepsy and provide a statistically significant pattern of topographic- and frequency-dependent brain–heart interactions for schizophrenic patients, as well as to show the differences from healthy control subjects. Suitable statistical models were found to quantify group differences. Significance: Generalized processing schemes and crucial points of pre-processing, adapted interaction analysis and performed statistical analysis are provided. The general concept of analyses is transferable also to other methods of interactions analysis and data representing even more complex physiological systems.
Incompatibility robustness of quantum measurements: a unified framework
Authors: Designolle Sébastien , Farkas Máté , Kaniewski Jędrzej
Published: October 2019

In quantum mechanics performing a measurement is an invasive process which generally disturbs the system. Due to this phenomenon, there exist incompatible quantum measurements, i.e. measurements that cannot be simultaneously performed on a single copy of the system. It is then natural to ask what the most incompatible quantum measurements are. To answer this question, several measures have been proposed to quantify how incompatible a set of measurements is, however their properties are not well-understood. In this work, we develop a general framework that encompasses all the commonly used measures of incompatibility based on robustness to noise. Moreover, we propose several conditions that a measure of incompatibility should satisfy, and investigate whether the existing measures comply with them. We find that some of the widely used measures do not fulfil these basic requirements. We also show that when looking for the most incompatible pairs of measurements, we obtain different answers depending on the exact measure. For one of the measures, we analytically prove that projective measurements onto two mutually unbiased bases are among the most incompatible pairs in every dimension. However, for some of the remaining measures we find that some peculiar measurements turn out to be even more incompatible.
Electromigration-induced resistance switching in indented Al microstrips
Authors: Lombardo J , Collienne S , Petrillo A , Fourneau E , Nguyen N D , Silhanek A V
Published: October 2019

Non-volatile resistive memory cells are promising candidates to tremendously impact the further development of Boolean and neuromorphic computing. In particular, nanoscale memory-bit cells based on electromigration (EM)-induced resistive switching in monolithic metallic structures have been identified as an appealing and competitive alternative to achieve ultrahigh density while keeping straightforward manufacturing processes. In this work, we investigate the EM-induced resistance switching in indented Al microstrips. In order to guarantee a large switching endurance, we limited the on-to-off ratio to a minimum readable value. Two switching protocols were tested, (i) a variable current pulse amplitude adjusted to ensure a precise change of resistance, and (ii) a fixed current pulse amplitude. Both approaches exhibit an initial training period where the mean value of the device’s resistance drifts in time, followed by a more stable behavior. Electron microscopy imaging of the devices show irreversible changes of the material properties from the early stages of the switching process. High and low resistance states show retention times of days and endurances of ∼103 switching cycles.
Increasing interannual variability of global vegetation greenness
Authors: Chen Chen , He Bin , Yuan Wenping , Guo Lanlan , Zhang Yafeng
Published: October 2019

Despite the long-term greening trend in global vegetation identified in previous investigations, changes in the interannual variability (IAV) of vegetation greenness over time is still poorly understood. Using Global Inventory Modeling and Mapping Studies normalized difference vegetation index (NDVI) third generation data and corresponding meteorological data from 1982 to 2015, we studied the changes and drivers of the IAV of vegetation greenness as indicated by the coefficient of variation of vegetation greenness at a global scale. Dry and high-latitude areas exhibited high NDVI variability whereas humid areas exhibited relatively low NDVI variability. We detected an increase in the global IAV of vegetation greenness over time using a 15 year moving window. Spatially, we observed significant increases in the IAV of vegetation greenness in greater than 45% of vegetated areas globally and decreases in 21%. Our comparison of ecological models suggests good performance in terms of simulating spatial differences in vegetation variability, but relatively poor performance in terms of capturing changes in the IAV of vegetation greenness. Furthermore, the dominant climate variables controlling changes in the IAV of vegetation greenness were determined spatially using principal component regression and partial least squares regression. The two methods yielded similar patterns, revealing that temperature exerted the biggest influence on changes in the IAV of vegetation greenness, followed by solar radiation and precipitation. This study provides insights into global vegetation variability which should contribute to an understanding of vegetation dynamics in the context of climate change.
Potential impact of climate change on solar resource in Africa for photovoltaic energy: analyses from CORDEX-AFRICA climate experiments
Authors: Bichet Adeline , Hingray Benoit , Evin Guillaume , Diedhiou Arona , Kebe Cheikh Mouhamed Fadel , Anquetin Sandrine
Published: October 2019

The development of renewable electricity in Africa could be massive in coming decades, as a response to the rapid rising electricity demand while complying with the Paris Agreements. This study shows that in the high-resolution climate experiments of CORDEX-AFRICA, the annual mean solar potential is expected to decrease on average by 4% over most of the continent by the end of the century, reaching up to 6% over the Horn of Africa, as a direct result of decrease in solar radiation and increase in air surface temperature. These projections are associated with large uncertainties, in particular over the Sahel and the elevated terrains of eastern Africa. While the expected decrease may affect the sizing of the numerous solar projects planned in Africa for the next decades, this study suggests that it does not endanger their viability. At last, this study indicates that the design of such projects also needs to account for the non-negligible uncertainties associated with the resource.
Feasibility of imaging amyloid in the brain using small-angle x-ray scattering
Authors: Choi Mina , Dahal Eshan , Badano Aldo
Published: October 2019

Small-angle x-ray scattering (SAXS) imaging may have the potential to image β-amyloid plaques in vivo in the brain without tracers for assessment of Alzheimer’s disease (AD). We use a laboratory SAXS system for planar imaging of AD model and control mouse brains slices to detect regions with high density of amyloid plaques. These regions were validated with histology methods. Using Monte Carlo techniques, we simulate SAXS computed tomography (SAXS-CT) system to study the potential of selectively differentiating amyloid targets in mouse and human head phantoms with detailed anatomy. We found contrast between amyloid and brain tissue at small q (below 0.8 nm−1) in the neocortex region of the transgenic brain slices as supported by histology. We observed similar behavior through planar SAXS imaging of an amyloid-like fibril deposit with a 0.8 mm diameter at a known location on a wild type mouse brain. In our SAXS-CT simulations, we found that 33-keV x rays provide increase plaque visibility in the mouse head for targets of at least 0.1 mm in diameter, while in the human head, 70-keV x rays were capable of detecting plaques as small as 2 mm. To increase radiation efficiency, we used a weighted-sum image visualization approach allowing the dose deposited by 70-keV x rays per SAXS-CT slice of the human head to be reduced by a factor of 10 to 71 mGy for gray matter and 63 mGy for white matter. The findings suggest that a dedicated SAXS-CT system for in vivo amyloid imaging in small animals and humans can be successfully developed with further system optimization to detect regions with amyloid plaques in the brain with a safe level of radiation dose.
Induced drag in two dimensions in ideal fluids
Authors: Meyer R W , Erland S
Published: October 2019

In this paper we suggest a model for how a significant part of the drag forces on two-dimensional objects can be derived using the circulation that is naturally maintained around the objects. We assume incompressible and inviscid potential flow and that the circulation is already generated. The resulting velocity field complements the one that is known to generate Prandtl’s induced drag in three dimensions. We demonstrate how fluid particles in a velocity field are attracted towards an object, and that this, due to conservation of momentum, results not only in lift, but also in drag forces. The magnitude of a disturbance velocity can be derived from the circulation of bound and shed vortices accompanying the object and parameters taken from the von Kármán vortex street description. Another part of the drag is generated by vortices that emerge behind blunt bodies when fluid particles do not follow the surface of the objects. We obtain a mathematical description of the resistance of several types of blunt bodies and rotating cylinders. The model involves no parameters that are derived from empirical data. Still, this inviscid approach corresponds well with experimental data in viscous flow and is close to a mathematical empirical description of rotating cylinders by W. G. Bickley.
Deep annotation of untargeted LC-MS metabolomics data with Binner
Authors: Kachman Maureen , Habra Hani , Duren William , Wigginton Janis , Sajjakulnukit Peter , Michailidis George , Burant Charles , Karnovsky Alla
Published: October 2019

Motivation

When metabolites are analyzed by electrospray ionization (ESI)-mass spectrometry, they are usually detected as multiple ion species due to the presence of isotopes, adducts and in-source fragments. The signals generated by these degenerate features (along with contaminants and other chemical noise) obscure meaningful patterns in MS data, complicating both compound identification and downstream statistical analysis. To address this problem, we developed Binner, a new tool for the discovery and elimination of many degenerate feature signals typically present in untargeted ESI-LC-MS metabolomics data.

Results

Binner generates feature annotations and provides tools to help users visualize informative feature relationships that can further elucidate the underlying structure of the data. To demonstrate the utility of Binner and to evaluate its performance, we analyzed data from reversed phase LC-MS and hydrophilic interaction chromatography (HILIC) platforms and demonstrated the accuracy of selected annotations using MS/MS. When we compared Binner annotations of 75 compounds previously identified in human plasma samples with annotations generated by three similar tools, we found that Binner achieves superior performance in the number and accuracy of annotations while simultaneously minimizing the number of incorrectly annotated principal ions. Data reduction and pattern exploration with Binner have allowed us to catalog a number of previously unrecognized complex adducts and neutral losses generated during the ionization of molecules in LC-MS. In summary, Binner allows users to explore patterns in their data and to efficiently and accurately eliminate a significant number of the degenerate features typically found in various LC-MS modalities.

Availability and implementation

Binner is written in Java and is freely available from http://binner.med.umich.edu.

Supplementary information

Supplementary data are available at Bioinformatics online.
Larvae of pyrrhocorid true bugs are not to spiders’ taste: putative Müllerian mimicry
Authors: Raška Jan , Krajíček Jan , Bosáková Zuzana , Štys Pavel , Exnerová Alice
Published: October 2019

Adults and larvae of a true bug, Scantius aegyptius (Heteroptera: Pyrrhocoridae), closely resemble sympatric firebugs, Pyrrhocoris apterus, and probably form a mimetic relationship with the latter species. Scantius aegyptius adults, although producing a secretion atypical of true bugs, are to some extent chemically protected against predators. In this study, we analysed the composition and function of the larval secretion in S. aegyptius, and investigated the mimetic relationship between larvae of S. aegyptius and P. apterus. The main component of the larval secretion in S. aegyptius is 2-heptanol, a chemical not known to function in anti-predatory defence, followed by (E)-2-octenal, a common defensive chemical of true bugs. When larvae of both species were presented to jumping spiders (Evarcha arcuata), S. aegyptius was slightly less well protected than P. apterus, but the spiders behaved towards the two species in a similar way: they quickly learned to avoid the bugs, but usually attacked them again on the second day. The spiders also generalized their learned avoidance from one true bug species to the other (with only slight asymmetry favouring S. aegyptius), suggesting that the bugs’ mimetic relationship is most probably Müllerian, being advantageous to both species.
A Method for the Determination of Veterinary Drugs from Different Therapeutic Classes in Animal Urine
Authors: Ramos-Payán María , Ocaña-González Juan Antonio , Fernández-Torres Rut , Bello-López Miguel Ángel
Published: October 2019

A rapid, precise and robust HPLC separation procedure has been developed and optimized for the determination of a series of drugs of different therapeutic classes: chlortetracycline, oxitetracycline, cefoperazone, diclofenac, tiamphenicol, marbofloxacin, ciprofloxacin, danofloxacin, enrofloxacin and flumequine. The chromatographic method used a monolithic C18 column and both diode array and fluorescence detection. This procedure was validated for the analysis of drugs in cow urine, using a simple and fast procedure with methanol/acetonitrile, allowing the simultaneous and efficient extraction of most of the studied drugs. The proposed method was successfully applied to the determination of enrofloxacin in cow urine, collected after the administration of this antibiotic.
From Heaven to Earth: ‘Cultural Idealism’ and ‘Moral Realism’ as Chinese Contributions to Global International Relations
Authors: Acharya Amitav
Published: October 2019

The discipline of International Relations (IR) is increasingly being criticized for ignoring and marginalizing states and societies outside of the core countries of the West. The idea of a ‘Global IR’ has been proposed since 2014 a pathway toward a bridging the ‘West and the Rest’ divide and thus develop a more inclusive discipline, recognizing its multiple and diverse foundations. At the same time, there is a trend toward developing theories, or ‘schools’, on a national or regional basis, the leading examples of which come from China. This article examines some theoretical constructs emerging in China, such as the ‘Relational Theory’ of Qin Yaqing, who is the foundational scholar in the ‘Chinese School of IR’, the Tianxia (‘all under Heaven’) concept as applied to IR and world order by Zhao Tingyang, and ‘Moral Realism’ of Yan Xuetong, who is the leading figure of the ‘Tsinghua School’. To many scholars, both inside and outside China, the relationship among the various Chinese approaches and their overall contribution to the IR field remain unclear. Without claiming to capture all their nuances and complexity, this article hopes to stimulate a conversation among scholars, Chinese and foreign, with a view to generate greater clarity and highlight their importance to the study of IR. I argue that while making important contributions, the Chinese approaches to International Relations Theory (IRT) also face a number of challenges. This includes the need for them to offer more convincing proof that the concepts and explanations they propose can apply to other societies and to IR more generally. Moreover, there is the need for these approaches to attract a critical mass of followers worldwide, stimulate a research agenda for other, especially younger scholars, and distance themselves from the official Chinese policy framings. The Global IR approach offers a helpful framework for highlighting and perhaps addressing these challenges, especially in avoiding cultural exceptionalism and ensuring their wider relevance beyond China.
Using stable isotopes to estimate migratory connectivity for a patchily distributed, wetland-associated Neotropical migrant
Authors: Reese Jessie , Viverette Catherine , Tonra Christopher M , Bayly Nicholas J , Boves Than J , Johnson Erik , Johnson Matthew , Marra Peter , Ames Elizabeth M , Caguazango Angela , DeSaix Matthew , Matthews Alix , Molina Alessandro , Percy Katie , Slevin Morgan C , Bulluck Lesley
Published: October 2019

Estimates of migratory connectivity are needed for full annual cycle population models of migratory bird species experiencing rapid declines in abundance. One technique to determine migratory connectivity is through stable isotope analysis. This low-resolution method may be influenced by how data are calibrated between isotopes measured in precipitation and those measured in feathers, and can be informed by incorporating relative abundance into the assignment model. eBird abundance maps are a new tool combining citizen science data into a predictive species distribution model. In the Prothonotary Warbler (Protonotaria citrea), a wetland-associated songbird with a patchy breeding distribution, we sought to use stable-hydrogen isotope analysis informed by a species-specific calibration equation and eBird abundance data to determine the strength of migratory connectivity. We developed a species-specific calibration equation using known-origin samples from the breeding grounds and found that stable-hydrogen isotope values measured in precipitation explained 50% of the variation in stable-hydrogen isotope values among feathers. We found that the assignment model incorporating eBird abundance data correctly identified the true origins of 66% of individuals, and that the average assignment area (as a measure of precision) was 64% of the breeding distribution. These results represented a 7% increase in precision and a 3% decrease in accuracy when compared to a model that was not informed by abundance. Based on these models, wintering populations from 6 countries represented a mix of likely breeding origins, suggesting low migratory connectivity for Prothonotary Warblers. We found evidence that wintering latitude was related to likely breeding origin, with individuals at western wintering locations more likely to have southern breeding origins, but this relationship was weak. These results corroborate studies using archival light-level geolocators and high-resolution genetic markers, which also demonstrated weak migratory connectivity in this species. For patchily distributed species, eBird abundance data may not provide a useful increase in precision and accuracy for isotope assignments.
What is the cause of hypotension? A rare complication of percutaneous coronary intervention of a chronic total occlusion: a case report
Authors: Vetrugno Vincenzo , Sharma Harish , Townend Jonathan N , Khan Sohail Q
Published: October 2019

Background

In the last few years, complex techniques and advanced equipment became available to treat chronically occluded coronary arteries. Such procedures portend a series of possible complications that operators should be ready to quickly recognize and deal with.

Case summary

A 75-year-old lady with uncontrolled stable angina underwent percutaneous treatment of a chronically occluded right coronary artery. After balloon angioplasty and stenting, she developed a severe hypotension, refractory to fluid resuscitation and vasopressors. Computerized tomography scan demonstrated an intramural haematoma (IMH) of the right atrioventricular groove resulting in life-threatening pseudotamponade (or dry tamponade), as further confirmed by cardiac magnetic resonance imaging (MRI). The decision was for conservative management and haemodynamic support by intra-aortic balloon pump. Clinically, the patient improved and was discharged a few days later. Follow-up MRI confirmed resolution of the IMH.

Discussion

Severe hypotension during percutaneous treatment of chronically occluded coronary arteries may be related to various causes. Differential diagnosis is thus important in this setting and should include IMH, a rare but potentially fatal complication as it may cause compression of cardiac chambers and lead to pseudotamponade. A high index of suspicion is required to diagnose IMH but there are no clear guidelines for management of such cases.
Undetected displacement of a subcutaneous implantable cardioverter-defibrillator lead. importance of performing a chest X-ray during the first weeks post-implant: a case report
Authors: Apolo Jose , San Antonio Rodolfo , Mont Lluís , Tolosana José María
Published: October 2019

Background

In recent years, subcutaneous implantable cardioverter-defibrillator (S-ICD) implants have progressively increased and have been shown to be safe and highly successful, affording low reintervention rates regardless of the technique used.

Case summary

We present a case of S-ICD implantation in a patient diagnosed with idiopathic ventricular fibrillation. In the first follow-up consultation the patient showed appropriate detection parameters in the three configurations. However, chest X-ray revealed lead displacement with a tip migration from the manubrium area of the sternum to the xiphoid process.

Discussion

This case highlights the importance of performing at least one chest X-ray during the first weeks after S-ICD implantation, allowing the detection of a problem such as lead displacement, which can lead to undersensing of ventricular arrhythmias or S-ICD oversensing.
Demand and supply of agricultural ES: towards benefit-based policy
Authors: Tienhaara Annika , Haltia Emmi , Pouta Eija , Arovuori Kyösti , Grammatikopoulou Ioanna , Miettinen Antti , Koikkalainen Kauko , Ahtiainen Heini , Artell Janne
Published: October 2019

In order to integrate ecosystem services (ES) in designing agri-environmental policy, we investigated both the demand for, and supply of, ES from agricultural environments in Finland. Using the discrete choice experiment method, we measured citizens’ willingness to pay (WTP) for four different ES and analysed farmers’ compensation request (willingness to accept [WTA]) for producing these services. Biodiversity and water quality gathered the highest WTA of farmers, but also the highest WTP of citizens. Overall, the average WTA exceeded the WTP for almost all attributes and levels, but 20–27 per cent of farmers were willing to produce the ES with the compensation lower than citizens’ WTP.
Stability of anticholinergic load in Australian community-dwelling older people: a longitudinal analysis
Authors: Mate Karen E , Barnett Michelle , Kerr Karen P , Pond C Dimity , Magin Parker J
Published: October 2019

Background

It is recommended that anticholinergic medication is avoided in older people, especially those with cognitive impairment.

Objective

To investigate anticholinergic load (ACL) over time in older primary care patients with and without cognitive impairment.

Methods

Community-dwelling general practice patients at baseline (n = 1768), at year one (n = 1373) and a restricted cohort (with possible or definite cognitive impairment) at year two (n = 370) had medication regimens documented by a research nurse during a home visit. Anticholinergic medicines were categorized as levels 1–3 (low-high potency) and summed for each participant as a measure of their ACL.

Results

Most participants had no change in ACL over time, but there was some turnover in the anticholinergic medications used. The mean change in ACL was 0.012 ± 0.99 from baseline to 12 months and −0.04 ± 1.3 from baseline to 24 months. Cardiovascular drugs were the most commonly used level 1 anticholinergics, followed by antidepressants and opioids. Antidepressants and urologicals were the most commonly used level 3 anticholinergics. The rate of anticholinergic deprescribing was equivalent to the rate of anticholinergic initiation, and commonly involved the level 1 drugs warfarin, furosemide and temazepam, and the level 3 drugs amitriptyline and oxybutynin. People with dementia had a higher ACL at baseline and year one compared with other participants.

Conclusion

ACL of community-dwelling older people was very stable over time. This may represent lost opportunities for deprescribing as well as potentially inappropriate prescribing, particularly in those with cognitive impairment.
Genetic interaction between Ptc2 and protein phosphatase 4 (PP4) in the regulation of DNA damage response and virulence in Candida albicans
Authors: Feng Jia , Shan Aidi , Hu Jing , Cao Zhenyu , Lv Rui , Feng Jinrong
Published: October 2019

In the pathogenic fungus Candida albicans, phosphoregulation of the checkpoint kinase Rad53 plays a crucial role in the filamentous growth response to genotoxic stresses. The protein phosphatase 4 (PP4) complex, containing Pph3 and either Psy2 or Psy4, is proved to play a critical role in Rad53 dephosphorylation. In previous studies, we characterized CaPtc2 (the ortholog of both Ptc2 and Ptc3 in Saccharomyces cerevisiae) as a potential DNA-damage-related protein phosphatase. In this study, we checked the genetic interaction of PTC2 with the PP4 complex in the DNA damage response pathway. The results suggest that Ptc2 shows a negative genetic interaction with Pph3, but positive genetic interaction with either Psy2 or Psy4 in response to genotoxic stress. Deletion of PTC2 alone resulted in no significant change in cell virulence, but double deletion of PTC2 PPH3 significantly decreased virulence, while double deletions of either PTC2 PSY2 or PTC2 PSY4 caused virulence levels similar to that shown by PSY2 or PSY4 single-gene deletion cells. Taken together, we propose that Ptc2 in C. albicans plays a compensatory role for Pph3 but is dependent on Psy2 and Psy4 in regulation of DNA damage and cell virulence.
Action research with parkrun UK volunteer organizers to develop inclusive strategies
Authors: Fullagar Simone , Petris Sandra , Sargent Julia , Allen Stephanie , Akhtar Muhsina , Ozakinci Gozde
Published: October 2019

This article addresses the challenge of promoting physical activity through a focus on equity and engaging physically inactive citizens through the development of inclusive strategies within parkrun UK—a free, volunteer-led, weekly mass community participation running event. We discuss how a UK-based action research design enabled collaboration with volunteer event organizers to understand participant experiences, constraints and develop localized inclusive practices. In contrast with ‘expert’-driven health behaviour interventions, our research pursued a ‘ground up’ approach by asking what can be learnt from the successes and challenges of organizing community events, such as parkrun UK, to promote inclusion? A modified participatory action research approach was used with four parkrun sites across England, Scotland and Northern Ireland, that involved quantitative and qualitative analysis of survey data (n = 655) that informed the process. Our analysis explored parkrunners’ and volunteer organizers’ perceptions relating to (i) the demographics of parkrun participation and (ii) actions for change in relation to the challenges of engaging marginalized groups (women, ethnic minorities, low income, older people, those with disabilities or illness). We discuss the challenges and opportunities for addressing (in)equity and inclusion through volunteer-based organizations and the implications for translating knowledge into organizational strategies.
Anastomotic Ulcers After Ileocolic Resection for Crohn’s Disease Are Common and Predict Recurrence
Authors: Hirten Robert P , Ungaro Ryan C , Castaneda Daniel , Lopatin Sarah , Sands Bruce E , Colombel Jean Frederic , Cohen Benjamin L
Published: October 2019

Background

Crohn’s disease recurrence after ileocolic resection is common and graded with the Rutgeerts score. There is controversy whether anastomotic ulcers represent disease recurrence and should be included in the grading system. The aim of this study was to determine the impact of anastomotic ulcers on Crohn’s disease recurrence in patients with prior ileocolic resections. Secondary aims included defining the prevalence of anastomotic ulcers, risk factors for development, and their natural history.

Methods

We conducted a retrospective cohort study of patients undergoing an ileocolic resection between 2008 and 2017 at a large academic center, with a postoperative colonoscopy assessing the neoterminal ileum and ileocolic anastomosis. The primary outcome was disease recurrence defined as endoscopic recurrence (>5 ulcers in the neoterminal ileum) or need for another ileocolic resection among patients with or without an anastomotic ulcer in endoscopic remission.

Results

One hundred eighty-two subjects with Crohn’s disease and an ileocolic resection were included. Anastomotic ulcers were present in 95 (52.2%) subjects. No factors were associated with anastomotic ulcer development. One hundred eleven patients were in endoscopic remission on the first postoperative colonoscopy. On multivariable analysis, anastomotic ulcers were associated with disease recurrence (adjusted hazard ratio [aHR] 3.64; 95% CI, 1.21–10.95; P = 0.02). Sixty-six subjects with anastomotic ulcers underwent a second colonoscopy, with 31 patients (79.5%) having persistent ulcers independent of medication escalation.

Conclusion

Anastomotic ulcers occur in over half of Crohn’s disease patients after ileocolic resection. No factors are associated with their development. They are associated with Crohn’s disease recurrence and are persistent.
A Quantum Algebra Approach to Multivariate Askey–Wilson Polynomials
Authors: Groenevelt Wolter
Published: October 2019

We study matrix elements of a change of basis between two different bases of representations of the quantum algebra ${\mathcal{U}}_q(\mathfrak{s}\mathfrak{u}(1,1))$. The two bases, which are multivariate versions of Al-Salam–Chihara polynomials, are eigenfunctions of iterated coproducts of twisted primitive elements. The matrix elements are identified with Gasper and Rahman’s multivariate Askey–Wilson polynomials, and from this interpretation we derive their orthogonality relations. Furthermore, the matrix elements are shown to be eigenfunctions of the twisted primitive elements after a change of representation, which gives a quantum algebraic derivation of the fact that the multivariate Askey–Wilson polynomials are solutions of a multivariate bispectral $q$-difference problem.
Beyond the hinterland: exploring the international actorness of China’s Yunnan province
Authors: Liu Tianyang , Song Yao
Published: October 2019

The scholarly literature on paradiplomacy has tended to focus overly on subnational governments in federal systems rather than those in unitary and centralized countries. However, it is important to note that some subnational governments in these countries have been increasingly proactive in international relations (IR). This study explores the paradiplomatic activities of Yunnan, a province in the south of China which, since the early 1990s, has actively engaged in cross-border cooperation. Combining the concept of paradiplomacy with the theory of actorness, this study first argues that Yunnan has been incentivized to conduct paradiplomacy through the decision of Beijing to open China’s borders; the inefficiency of the central government in managing border-related issues; and the interprovincial competition over economic and diplomatic clouts in the Mekong subregion. Second, this study argues that Yunnan’s new external affairs powers have been consolidated by a host of new opportunities stemming from the external environment. Third, it argues that Yunnan’s new external affairs powers have enabled it to leverage two broad instruments (infrastructure development and economic statecraft) to make neighboring countries more dependent on cooperation with it. And finally, it is argued that Yunnan’s role as an IR player has been acknowledged both by neighboring countries and by Beijing.
Bridging the digital divide: Older adults’ engagement with online cinema heritage
Authors: Dibeltulo Silvia , Culhane Sarah , Treveri Gennari Daniela
Published: October 2019

Is there a way to ensure older adults can bridge the digital divide and engage with online cultural heritage? How can cinema-going memories encourage cross-generational engagement? This article proposes to address these issues by using the Italian Cinema Audiences research project as a case study, and specifically cinema-going memories as intangible cultural heritage (Ercole et al., 2016, Cinema heritage in Europe: preserving and sharing culture by engaging with film exhibition and audiences. Editorial. Alphaville: Journal of Film and Screen Media, 11(Summer): 1–12. Web. ISSN: 2009-4078). It aims to tackle the difficulty of engaging the older generation with the digital world, by proposing and testing new ways to resolve it. Through a mixed-methods ethnographic approach, this article investigates different strategies: the use of social media platforms; a cross-generational activity involving Historypin, a digital, user-generated archive of crowdsourced historical material; an online dedicated archive built in collaboration with the older adults involved in the project. These different solutions aim not only at increasing digital engagement among older adults, but also at furthering younger generations’ involvement in shared cultural heritage in an online context. By focusing on the memories of cinema-going in 1950s Italy, the article explores the implications of the advantages and disadvantages of these different approaches. It also tests Anja K. Leist’s research findings (2013, Social media use of older adults: a mini-review. Gerontology, 59(4): 378–84) on the key role of moderators (the younger generation) to help novice users (the older generation) in the ‘continuous engagement’ in digital environments. We conclude that in order to bridge the digital divide two components are necessary simultaneously: the creation of digital platforms in which the older generations are both curators and users, and the support of and interaction with younger generations.
Knowledge, attitudes, and practices regarding coccidioidomycosis among healthcare providers in four counties in Washington State, 2017
Authors: Hubber J , Person A , Jecha L , Flodin-Hursh D , Stiffler J , Hill H , Bassham S , McCotter O Z , Oltean H N
Published: October 2019

Coccidioidomycosis is an emerging infection in Washington State. The epidemiology of the disease in Washington is poorly understood at present; underrecognition and underreporting of coccidioidomycosis is suspected based on reports of only severe disease. We sought to characterize healthcare provider knowledge, attitudes, and practices regarding coccidioidomycosis awareness, diagnosis, and treatment in south-central Washington. We conducted a cross-sectional survey of actively practicing healthcare providers in four counties in south-central Washington, an area recently described as endemic for Coccidioides. Survey results were used to assess awareness of reporting requirements, confidence in ability to diagnose and treat, confidence that knowledge is current, calculated knowledge score, and consideration of risk in patient population. The majority of respondents were unaware of the reporting requirement for coccidioidomycosis in Washington and further unaware that the disease had been reported in the state. Less than a third of survey respondents reported confidence in their ability to diagnose coccidioidomycosis and confidence that their knowledge is current. The majority of respondents never or rarely consider a diagnosis of coccidioidomycosis, and <25% of respondents indicated a working knowledge of serologic tests for the infection. The average knowledge score for respondents was 65%. Previous education, training, or practice regarding coccidioidomycosis was the only identified predictor of confidence and consideration of risk. These data indicate the substantial need for education and training among healthcare providers in south-central Washington and support the concern that a small proportion of existing cases of coccidioidomycosis are reported to the health department.
Spiral-arm instability – III. Fragmentation of primordial protostellar discs
Authors: Inoue Shigeki , Yoshida Naoki
Published: October 2019

We study the gravitational instability and fragmentation of primordial protostellar discs by using high-resolution cosmological hydrodynamics simulations. We follow the formation and evolution of spiral arms in protostellar discs, examine the dynamical stability, and identify a physical mechanism of secondary protostar formation. We use linear perturbation theory based on the spiral-arm instability (SAI) analysis in our previous studies. We improve the analysis by incorporating the effects of finite thickness and shearing motion of arms, and derive the physical conditions for SAI in protostellar discs. Our analysis predicts accurately the stability and the onset of arm fragmentation that is determined by the balance between self-gravity and gas pressure plus the Coriolis force. Formation of secondary and multiple protostars in the discs is explained by the SAI, which is driven by self-gravity and thus can operate without rapid gas cooling. We can also predict the typical mass of the fragments, which is found to be in good agreement with the actual masses of secondary protostars formed in the simulation.