bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of naturally occurring inhibitors in Xian-Ling-Gu-Bao capsule against the glucuronidation of estrogens
Authors: He Liangliang , Xu Chunxia , Wang Ziying , Duan Shuyi , Xu Jinjin , Li Chuan , Yao Xinsheng , Gonzalez Frank J. , Qin Zifei , Yao Zhihong
Published: August 2022

Xian-Ling-Gu-Bao (XLGB) capsule, a well-known traditional Chinese medicine prescription, is widely used for the treatment of osteoporosis. It could significantly increase the levels of estrogen in ovariectomized rats and mice. However, this working mechanism has not been well elucidated. Considering that UDP-glucuronosyltransferase (UGT) enzymes are the important enzymes that inactivate and regulate estrogen activity in vivo, this study aimed to identify the bioactive compounds from XLGB against the glucuronidation of estrogens. First, thirty compounds were considered as candidate bioactive compounds based on our previous studies including pharmacological evaluation, chemical profiles, and metabolic profiles. Second, the characteristics of estrogen glucuronidation by uridine diphosphate glucuronic acid (UDPGA)-supplemented human liver microsomes (HLM), human intestine microsomes (HIM), and expressed UGT enzymes were determined, and the incubation systems of their key UGT enzymes were optimized. Then, inhibitory effects and mechanisms of XLGB and its main compounds toward the key UGT isozymes were further investigated. As a result, estrogen underwent efficient glucuronidation by HLM and HIM. UGT1A10, 1A1, and 2B7 were mainly responsible for the glucuronidation of estrone, β-estradiol, and estriol, respectively. For E1 and E2, UGT1A10 and 1A1 tended to mediate estrogen-3-O-glucuronidation, while UGT2B7 preferred catalyzing estrogen-16-O-glucuronidation. Furthermore, the incubation system for active UGT isoforms was optimized including Tris-HCl buffer, detergents, MgCl2 concentration, β-glucuronidase inhibitors, UDPGA concentration, protein concentration, and incubation time. Based on optimal incubation conditions, eleven, nine, and nine compounds were identified as the potent inhibitors for UGT1A10, 1A1, and 2B7, respectively (IC50 < 4.97 μM and Ki < 3.35 μM). Among them, six compounds (bavachin, isobavachin, isobavachalcone, neobavaisoflavone, corylifol A, and icariside II) simultaneously demonstrated potent inhibitory effects against these three active enzymes. Prenylated flavanols from Epimedium brevicornu Maxim., prenylated flavonoids from Psoralea corylifolia L., and salvianolic acids from Salvia miltiorrhiza Bge. were characterized as the most important and effective compounds. The identification of potent natural inhibitors of XLGB against the glucuronidation of estrogen laid an important foundation for the pharmacodynamic material basis.
Preparation and Evaluation of the Cytoprotective Activity of Micelles with DSPE-PEG-C60 as a Carrier Against Doxorubicin-Induced Cytotoxicity
Authors: Xu Beihua , Ding Zhongpeng , Hu Ying , Zhang Ting , Shi Senlin , Yu Guangmao , Qi Xuchen
Published: August 2022

To deliver doxorubicin (DOX) with enhanced efficacy and safety in vivo, fullerenol-modified micelles were prepared with the amphiphilic polymer DSPE-PEG-C60 as a carrier, which was synthesized by linking C60(OH)22 with DSPE-PEG-NH2. Studies of its particle size, PDI, zeta potential, and encapsulation efficiency were performed. DOX was successfully loaded into the micelles, exhibiting a suitable particle size [97 nm, 211 nm, 260 nm, vector: DOX = 5:1, 10:1; 15:1 (W/W)], a negative zeta potential of around −30 mv, and an acceptable encapsulation efficiency [86.1, 95.4, 97.5%, vector: DOX = 5:1, 10:1; 15:1 (W/W)]. The release behaviors of DOX from DSPE-PEG-C60 micelles were consistent with the DSPE-PEG micelles, and it showed sustained release. There was lower cytotoxicity of DSPE-PEG-C60 micelles on normal cell lines (L02, H9c2, GES-1) than free DOX and DSPE-PEG micelles. We explored the protective role of DSPE-PEG-C60 on doxorubicin-induced cardiomyocyte damage in H9c2 cells, which were evaluated with a reactive oxygen species (ROS) assay kit, JC-1, and an FITC annexin V apoptosis detection kit for cellular oxidative stress, mitochondrial membrane potential, and apoptosis. The results showed that H9c2 cells exposed to DSPE-PEG-C60 micelles displayed decreased intracellular ROS, an increased ratio of red fluorescence (JC-1 aggregates) to green fluorescence (JC-1 monomers), and a lower apoptotic ratio than the control and DSPE-PEG micelle cells. In conclusion, the prepared DOX-loaded DSPE-PEG-C60 micelles have great promise for safe, effective tumor therapy.
I-κB kinase-ε deficiency improves doxorubicin-induced dilated cardiomyopathy by inhibiting the NF-κB pathway
Authors: Liu Yafeng , Xu Yueyue , Yao Yiwei , Cao Yide , Chen Ganyi , Cai Yuchen , Chen Wen , Chen Xin , Qiu Zhibing
Published: August 2022

Dilated cardiomyopathy (DCM) can lead to heart expansion and severe heart failure, but its specific pathogenesis is still elusive. In many cardiovascular diseases, I-κB kinase-ε (IKKε) has been recognized as a pro-inflammatory molecule. In this study, wild-type mice (WT, n = 14) and IKKε knockout mice (IKKε-KO, n = 14) were intraperitoneally injected with a cumulative dose of 25 mg/kg with Dox or Saline five times in 30 days. Finally, the experimental mice were divided into WT + Saline group、WT + DOX group、IKKε-KO + Saline group and IKKε-KO + Dox group. Echocardiography was performed to assess cardiac structure and function. Moreover, the mechanism was validated by immunohistochemistry and western blotting. Our results demonstrated that compared to WT + Dox mice, IKKε-KO + Dox mice exhibited attenuation of dilated cardiomyopathy-related morphological changes and alleviation of heart failure. Additionally, compared to the WT mice after Dox-injected, the expression of fibrosis and proinflammatory were decreased in IKKε-KO mice, and the expression of cardiac gap junction proteins was much higher in IKKε-KO mice. Further testing found that pyroptosis and apoptosis in the myocardium were also ameliorated in IKKε-KO mice compared to WT mice after Dox was injected. Mechanistically, our results showed that deficiency of IKKε might inhibit the phosphorylation of IκBα, p65, RelB, and p100 in mouse heart tissues after Dox stimulation. In summary, our research suggests that IKKε might play an essential role in the development of Dox-induced dilated cardiomyopathy and may be a potential target for the treatment of dilated cardiomyopathy in the future.
Synergism of endophytic Bacillus subtilis and Klebsiella aerogenes modulates plant growth and bacoside biosynthesis in Bacopa monnieri
Authors: Shukla Namita , Singh Deepti , Tripathi Arpita , Kumari Poonam , Gupta Rahul Kumar , Singh Shiwangi , Shanker Karuna , Singh Akanksha
Published: August 2022

Bacopa monnieri is the main source of pharmaceutically important bacosides; however, the low content of these molecules in planta remains a limiting factor for fulfilling the industrial requirement. The accumulation of secondary metabolites can be enhanced in plants upon inoculation with endophytes. In this study, we isolated and analyzed the culturable endophytes associated with different plant parts. By analyzing their impact on plant growth parameters (in vitro and in vivo) and Bacoside A content, we found few candidates which increased bacoside accumulation significantly. Finally, two promising endophytes namely Bacillus subtilis (OK070745) and Klebsiella aerogenes (OK070774) were co-cultivated with B. monnieri cuttings singly and in combination mode to clarify their effect on bacoside biosynthesis and their accumulation in B. monnieri shoot. Consortium-inoculated plants significantly enhanced the plant biomass and Bacoside A content with respect to single inoculation. The results of real-time quantitative (RT-PCR) revealed significant accumulation of bacoside biosynthetic pathway transcripts (HMGCR, PMVK, FDPS, SQS, and β-AS) in the case of plants inoculated with microbial combination, while the single inoculation of B. subtilis diverted the plant’s machinery toward the synthesis of phenylpropanoid genes like CCR, CAD, CHS, and HST. In addition, higher expression of MYB 2 and WRKY 1 transcription factors in combinational treatment points out their probable role in better physiological and developmental processes. Altogether, this is the first study on B. monnieri-endophyte interaction showing improvement in the accumulation of bacoside A by modulating various genes of metabolic pathway and thus suggests an effective “green approach” for augmenting in planta production of pharmaceutically important bacosides.
Temporal dynamics of sugar beet (Beta vulgaris L.) N supply from cover crops differing in biomass quantity and composition
Authors: Koch Heinz-Josef , Grunwald Dennis , Essich Lisa , Ruser Reiner
Published: August 2022

Cover crops are supposed to decrease the soil mineral N content (Nmin) during winter and increase the N supply to subsequent main crops due to mineralization of N previously prevented from leaching. However, data on N supply from cover crops grown before sugar beet have rarely been reported for Central European conditions. Therefore, our study aimed to provide information for cover crops differing in frost resistance and biomass quantity applicable for N fertilizer dressing in the subsequent main crop. In 2018/19 and 2019/20, field trials were conducted on two Luvisol sites in Germany typical for sugar beet cultivation, comprising a sequence of autumn sown cover crops grown after field pea followed by unfertilized sugar beet main crops sown in next spring. Apparent net N mineralization and the N effect of cover crops on sugar beet were calculated according to a mass balance approach including Nmin and sugar beet N uptake. Winter rye and oil radish revealed the greatest potential for scavenging nitrate from the soil profile while reductions caused by frost-sensitive saia oat and spring vetch were more variable. The amount of N in the cover crop biomass was negatively correlated with Nmin in autumn and also in spring. Thus, for environmentally effective cover cropping in Central Europe, species with a sufficiently high frost tolerance should be chosen. Despite cover crop N uptake up to 170 kg N ha−1 and C:N ratios < 20, a positive N effect on sugar beet was only found between March and July of the beet growing season and was 50 kg N ha−1 at maximum, while between August and September, net immobilization was predominant with up to 100 kg N ha−1. Differences among crop species were not consistent across the site/years investigated. Sugar yield was lowest after rye at 3 sites/years and correlated positively with Nmin in spring. Correlation between yield and cover crop N effect was mostly low and inconsistent and could not be improved by a multiple regression approach. Thus, factors other than in-season N supply from cover crops apparently impacted sugar beet yield formation to a larger extent.
Identification of promising chickpea interspecific derivatives for agro-morphological and major biotic traits
Authors: Singh Mohar , Kumar Tapan , Sood Salej , Malhotra Nikhil , Rani Upasana , Singh Sarvjeet , Singh Inderjit , Bindra Shayla , Kumar Sanjeev , Kumar Sandeep
Published: August 2022

The wild Cicer species is well-known for having climate-resilient and productivity-enhancing traits of interest. Therefore, wide hybridization could be used as a realistic strategy for introgressing prospective traits from wild species into the cultivated gene pool. The present study was, thus, undertaken to evaluate F7 chickpea interspecific derivatives derived from Cicer reticulatum Ladiz. and C. echinospermum P. H. Davis wild annual Cicer species. As a result, a set of six interspecific crosses were advanced using the single seed descent (SSD) method of breeding. The F7 generation of these crosses was assessed in two diverse agro-ecological regions of India. The data revealed a wide range of variation with respect to seed yield and its important component traits, which resulted in the identification of the most promising derivatives carrying desirable characters as indicated by range, mean, and coefficient of variation. Further, fruitful heterosis was also estimated as promising selection criteria for identifying superior lines for earliness and high seed yield, including resistance against prevailing stresses (ascochyta blight, botrytis gray mold, dry root rot, and fusarium wilt). The superior derivatives carrying putative characters could be recommended for further breeding and selection of genetic materials for developing suitable genotypes.
Tissue-targeted inorganic pyrophosphate hydrolysis in a fugu5 mutant reveals that excess inorganic pyrophosphate triggers developmental defects in a cell-autonomous manner
Authors: Gunji Shizuka , Kawade Kensuke , Tabeta Hiromitsu , Horiguchi Gorou , Oikawa Akira , Asaoka Mariko , Hirai Masami Yokota , Tsukaya Hirokazu , Ferjani Ali
Published: August 2022

Excess PPi triggers developmental defects in a cell-autonomous manner. The level of inorganic pyrophosphate (PPi) must be tightly regulated in all kingdoms for the proper execution of cellular functions. In plants, the vacuolar proton pyrophosphatase (H+-PPase) has a pivotal role in PPi homeostasis. We previously demonstrated that the excess cytosolic PPi in the H+-PPase loss-of-function fugu5 mutant inhibits gluconeogenesis from seed storage lipids, arrests cell division in cotyledonary palisade tissue, and triggers a compensated cell enlargement (CCE). Moreover, PPi alters pavement cell (PC) shape, stomatal patterning, and functioning, supporting specific yet broad inhibitory effects of PPi on leaf morphogenesis. Whereas these developmental defects were totally rescued by the expression of the yeast soluble pyrophosphatase IPP1, sucrose supply alone canceled CCE in the palisade tissue but not the epidermal developmental defects. Hence, we postulated that the latter are likely triggered by excess PPi rather than a sucrose deficit. To formally test this hypothesis, we adopted a spatiotemporal approach by constructing and analyzing fugu5-1 PDF1pro::IPP1, fugu5-1 CLV1pro::IPP1, and fugu5-1 ICLpro::IPP1, whereby PPi was removed specifically from the epidermis, palisade tissue cells, or during the 4 days following seed imbibition, respectively. It is important to note that whereas PC defects in fugu5-1 PDF1pro::IPP1 were completely recovered, those in fugu5-1 CLV1pro::IPP1 were not. In addition, phenotypic analyses of fugu5-1 ICLpro::IPP1 lines demonstrated that the immediate removal of PPi after seed imbibition markedly improved overall plant growth, abolished CCE, but only partially restored the epidermal developmental defects. Next, the impact of spatial and temporal removal of PPi was investigated by capillary electrophoresis time-of-flight mass spectrometry (CE-TOF MS). Our analysis revealed that the metabolic profiles are differentially affected among all the above transgenic lines, and consistent with an axial role of central metabolism of gluconeogenesis in CCE. Taken together, this study provides a conceptual framework to unveil metabolic fluctuations within leaf tissues with high spatio–temporal resolution. Finally, our findings suggest that excess PPi exerts its inhibitory effect in planta in the early stages of seedling establishment in a tissue- and cell-autonomous manner.
Genome-Wide Identification and Functional Analysis of Polyamine Oxidase Genes in Maize Reveal Essential Roles in Abiotic Stress Tolerance
Authors: Xi Yan , Hu Wenjing , Zhou Yue , Liu Xiang , Qian Yexiong
Published: August 2022

Polyamines (PAs) play a critical role in growth and developmental processes and stress responses in plants. Polyamine oxidase (PAO) is a flavin adenine dinucleotide (FAD)-dependent enzyme that plays a major role in PA catabolism. Here, for the first time, PAO genes in maize were screened for the whole genome-wide and nine ZmPAO genes were identified in this study, named as ZmPAO1-9. Based on structural characteristics and a comparison of phylogenetic relationships of PAO gene families from seven representative species, all nine PAO proteins in maize were categorized into three distinct subfamilies. Further, chromosome location and schematic structure revealed an unevenly distribution on chromosomes and evolutionarily conserved structure features of ZmPAO genes in maize, respectively. Furthermore, transcriptome analysis demonstrated that ZmPAO genes showed differential expression patterns at diverse developmental stages of maize, suggesting that these genes may play functional developmental roles in multiple tissues. Further, through qRT-PCR validation, these genes were confirmed to be responsive to heat, drought and salinity stress treatments in three various tissues, indicating their potential roles in abiotic stress responses. Eventually, to verify the biological function of ZmPAO genes, the transgenic Arabidopsis plants overexpressing ZmPAO6 gene were constructed as a typical representative to explore functional roles in plants. The results demonstrated that overexpression of ZmPAO6 can confer enhanced heat tolerance through mediating polyamine catabolism in transgenic Arabidopsis, which might result in reduced H2O2 and MDA accumulation and alleviated chlorophyll degradation under heat stress treatment, indicating that ZmPAO6 may play a crucial role in enhancing heat tolerance of transgenic Arabidopsis through the involvement in various physiological processes. Further, the expression analysis of related genes of antioxidant enzymes including glutathione peroxidase (GPX) and ascorbate peroxidase (APX) demonstrated that ZmPAO6 can enhance heat resistance in transgenic Arabidopsis through modulating heat-induced H2O2 accumulation in polyamine catabolism. Taken together, our results are the first to report the ZmPAO6 gene response to heat stress in plants and will serve to present an important theoretical basis for further unraveling the function and regulatory mechanism of ZmPAO genes in growth, development and adaptation to abiotic stresses in maize.
Limited effect of thermal pruning on wild blueberry crop and its root-associated microbiota
Authors: Morvan Simon , Paré Maxime C. , Schmitt Anne , Lafond Jean , Hijri Mohamed
Published: August 2022

Thermal pruning was a common pruning method in the past but has progressively been replaced by mechanical pruning for economic reasons. Both practices are known to enhance and maintain high yields; however, thermal pruning was documented to have an additional sanitation effect by reducing weeds and fungal diseases outbreaks. Nevertheless, there is no clear consensus on the optimal fire intensity required to observe these outcomes. Furthermore, fire is known to alter the soil microbiome as it impacts the soil organic layer and chemistry. Thus far, no study has investigated into the effect of thermal pruning intensity on the wild blueberry microbiome in agricultural settings. This project aimed to document the effects of four gradual thermal pruning intensities on the wild blueberry performance, weeds, diseases, as well as the rhizosphere fungal and bacterial communities. A field trial was conducted using a block design where agronomic variables were documented throughout the 2-year growing period. MiSeq amplicon sequencing was used to determine the diversity as well as the structure of the bacterial and fungal communities. Overall, yield, fruit ripeness, and several other agronomical variables were not significantly impacted by the burning treatments. Soil phosphorus was the only parameter with a significant albeit temporary change (1 month after thermal pruning) for soil chemistry. Our results also showed that bacterial and fungal communities did not significantly change between burning treatments. The fungal community was dominated by ericoid mycorrhizal fungi, while the bacterial community was mainly composed of Acidobacteriales, Isosphaerales, Frankiales, and Rhizobiales. However, burning at high intensities temporarily reduced Septoria leaf spot disease in the season following thermal pruning. According to our study, thermal pruning has a limited short-term influence on the wild blueberry ecosystem but may have a potential impact on pests (notably Septoria infection), which should be explored in future studies to determine the burning frequency necessary to control this disease.
Factors affecting expert performance in bid evaluation: An integrated approach
Authors: Wang Li , Ye Kunhui , Liu Yu , Wang Wenjing
Published: August 2022

Experts play a crucial role in underpinning decision-making in most management situations. While recent studies have disclosed the impacts of individuals’ inherent cognition and the external environment on expert performance, these two-dimensional mechanisms remain poorly understood. In this study, we identified 14 factors that influence expert performance in a bid evaluation and applied cross-impact matrix multiplication to examine the interdependence of the factors. The results indicate that the two dimension-related factors affect each other within a person–environment system, and a poor situation perception gives rise to the deviation of expert performance. Expert performance can be improved if external supervision and expertise are strengthened through deliberate practices. The study proposes a new expert performance research tool, elucidates its mechanism in bid evaluation from a cognitive psychology perspective, and provides guidelines for its improvement in workplace contexts.
Remote workers’ free associations with working from home during the COVID-19 pandemic in Austria: The interaction between children and gender
Authors: Hartner-Tiefenthaler Martina , Zedlacher Eva , el Sehity Tarek Josef
Published: August 2022

Empirical evidence from the COVID-19 pandemic shows that women carried the major burden of additional housework in families. In a mixed-methods study, we investigate female and male remote workers’ experiences of working from home (WFH) during the pandemic. We used the free association technique to uncover remote workers’ representations about WFH (i.e., workers’ reflection of subjective experiences). Based on a sample of 283 Austrian remote workers cohabitating with their intimate partners our findings revealed that in line with traditional social roles, men and women in parent roles are likely to experience WFH differently: Mothers’ representations about WFH emphasize perceived incompatibility between the work and non-work sphere whereas fathers’ representations highlight work-family facilitation of WFH. However, gender differences were also prevalent for women and men without children: Women seem to particularly benefit from more concentration at home, whereas men consider WFH as more efficient, practical and leading to less work. Thus, our findings imply that gender affected perceptions of WFH during the pandemic independently from children, but children seemed to increase the existing burden, in particular for women. To conclude, WFH can generally be seen as an enabler to reduce work-life/family conflict for both women and men, but bears different challenges based on the contextual (family) situation.
The influence of customer trust and artificial intelligence on customer engagement and loyalty – The case of the home-sharing industry
Authors: Chen Ying , Prentice Catherine , Weaven Scott , Hisao Aaron
Published: August 2022

Trust is an essential factor in online and offline transactions. However, the role of customer trust has received limited attention in the home-sharing economy. Drawing on the revised stimulus organism response model and trust transfer theory, this paper examines how customer trust in home-sharing hosts and platforms affects customer relationships, manifested in customer engagement and loyalty. As artificial intelligence (AI) is extensively utilized within home-sharing platforms to facilitate business operations and enhance the customer experience, this study also examines the influence of AI on customer trust and other related outcomes. The research was undertaken in China, with respondents who had used home-sharing platforms. Results from structural equation modeling show that customer trust had a significant positive relationship with customer engagement and loyalty. Customer engagement mediates the relationship between trust and loyalty, while AI may have a negative moderating effect between host trust and customer engagement and customer engagement and loyalty. The paper contributes to marketing, sharing economy and AI research. The work has implications for practitioners offering suggestions to develop marketing strategies for business growth and sustainability.
The work-family interface and the COVID-19 pandemic: A systematic review
Authors: Vitória Beatriz de Araújo , Ribeiro Maria Teresa , Carvalho Vânia Sofia
Published: August 2022

In an unprecedented fashion, COVID-19 has impacted the work-family interface since March 2020. As one of the COVID-19 pandemic consequences, remote work became widely adopted. Furthermore, it is expected that other pandemics will occur in the future. Hence, this context represents a chance to gain deeper insight into telecommuters’ work and family spheres. Following PRISMA guidelines, the present narrative review aims to synthesise the COVID-19 impact on the work-family interface. Out of 121 screened references, 32 articles that measure at least one of the following variables–work-family conflict (25), work-family enrichment (3), work-family balance (8), and boundary management (21) were included. A thematic analysis using NVIVO12 was conducted, from which eight topics emerged: “paid workload, unpaid workload, and gender”; “well-being and gender”; “job resources, job demands, and gender”; “couples and gender”; “parenting and gender”; “occurrence of work-family enrichment with work-family conflict and gender”; “enforced blurred boundaries, its management, and gender”; “boundary management impact on work-family conflict, work-family enrichment, and work-family balance.” Overall, studies point out that COVID-19 had a complex effect on both work-family conflict and work-family balance, making it difficult to state whether these variables were mitigated or augmented. Findings demonstrated that COVID-19 produced little changes in work-family enrichment. As for the COVID-19 impact on work-family boundary management, individuals had to create new tactics to manage them due to the absence of boundaries between both systems. Besides, due to traditional gendered roles, the COVID-19 health crisis seems to have brought additional hurdles to couples and women.

Systematic Review Registration

[https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021278254], identifier [CRD42021278254].
Ameliorated New Media Literacy Model Based on an Esthetic Model: The Ability of a College Student Audience to Enter the Field of Digital Art
Authors: Xu Rui , Wang Chen , Hsu Yen
Published: August 2022

In the current digital environment, people can visit every corner of the world without leaving their homes. New media technology compresses distance and time, but it also subverts the traditional mode of audience presence. Many traditional, offline content expression modes are also moving toward the digital field, and digital art is among them. Digital new media is a new art form that requires its audience to have a new media literacy; this literacy is necessary for esthetic experience and for audience participation. At present, the relatively lack of objective methodology for scientific research on aesthetic and media literacy has limited our current understanding. Therefore, we need to develop a new model and conduct empirical research with college students as the audience. Empirical research was conducted with an audience of college students. The study had the following purposes: (1) to add a new dimension to the esthetic model, namely new media literacy, to align the model with the current digital environment, and (2) to test the moderating effect of new media literacy on esthetic emotion as represented by interest and confusion. The experiment verified the study’s hypothesis that higher new media literacy was associated with higher esthetic interest and lower confusion. By contrast, has a substantial influence on the cognitive processes in humans, lower new media literacy was associated with lower esthetic interest and higher confusion. New media literacy is an essential quality for contemporary audiences. This knowledge may be useful for effective design. It provides a traditional and favorable learning environment and empirical reference for the subsequent improvement of digital aesthetics and media literacy.
Effectiveness of interventions for children and adolescents with autism spectrum disorder in high-income vs. lower middle-income countries: An overview of systematic reviews and research papers from LMIC
Authors: Pervin Maleka , Ahmed Helal Uddin , Hagmayer York
Published: August 2022

Background

There is a multitude of systematic reviews of interventions for children and adolescents with autism spectrum disorder (ASD). However, most reviews seem to be based on research conducted in High-Income Countries (HIC). Thus, summary findings may not directly apply to Lower Middle-Income Countries (LMIC). Therefore, we conducted a Meta-Review analyzing systematic reviews on the effectiveness of interventions for target outcomes in children and adolescents with ASD to find out whether there are differences in effectiveness between HIC and LMIC and which interventions can be considered evidence-based in LMIC.

Methods

Electronic databases (PsycINFO, PubMed, Cochrane database of systematic reviews) were searched for reviews on interventions for ASD in children and adolescents from January 2011 through December 2021, which included studies not coming from HIC. Systematic reviews with qualitative and quantitative syntheses of findings were included. Two investigators independently assessed studies against predetermined inclusion/exclusion criteria and extracted relevant data including quality and evidence assessments. Evidence for different types of interventions in HIC vs. LMIC was planned to be compared, but none of the reviews assessed potential differences. Therefore, a narrative review of the studies from LMIC was conducted including an assessment of quality and evidence.

Results

Thirty-five reviews fulfilled the inclusion criteria. Eleven considered findings from HIC and LMIC. Sixty-nine percent included studies with various research designs; 63% provided a qualitative synthesis of findings; 77% percent assessed the quality of studies; 43% systematically assessed the level of evidence across studies. No review compared evidence from HIC and LMIC. A review of the studies from LMIC found some promising results, but the evidence was not sufficient due to a small number of studies, sometimes poor quality, and small sample sizes.

Conclusion

Systematic reviews on interventions for children and adolescents with ASD did not look for potential differences in the effectiveness of interventions in HIC and LMIC. Overall, there is very little evidence from LMIC. None of the interventions can be considered evidence-based in LMIC. Hence, additional research and mutually agreed methodological standards are needed to provide a more secure basis for evidence-based treatments in LMIC trying to establish evidence-based practices.
The association between digitalization and mental health: The mediating role of wellbeing at work
Authors: Sun Jianmin , Shen Hongzhou , Ibn-ul-Hassan Syed , Riaz Amir , Domil Aura Emanuela
Published: August 2022

The study aims to measure the mediating relationship of wellbeing at work between digitalization (IT infrastructure, IT business spanning, and IT proactive stance) and their effect on mental health. The study uses primary data collection techniques to gather data and used purposive sampling to analyze the data. The sample size of the study is 349 respondents. The research uses Smart PLS software to measure the relationship through bootstrapping and algorithms. The study finds a significant positive mediating role of wellbeing between digitalization (IT infrastructure, IT business spanning, and IT proactive stance) and their effect on mental health. The study outcomes are helpful for managers and policymakers.
A gamified augmented reality vocational training program for adults with intellectual and developmental disabilities: A pilot study on acceptability and effectiveness
Authors: Tan Bhing-Leet , Guan Frank Yunqing , Leung Ivy Mun Wah , Kee Sharon Yi-May , Devilly Oran Zane , Medalia Alice
Published: August 2022

Objectives

The Augmented Reality Games to Enhance Vocational Ability of Patients (REAP) was an augmented reality vocational training program that provided skills training in the context of a psychiatric rehabilitation program. It was implemented over 10 weeks and consisted of gamified augmented reality café training scenarios and bridging group activities to facilitate transfer of learning to the work context. This pilot study aimed to explore the acceptability and effectiveness of the REAP program when carried out with adults with intellectual and developmental disabilities attending work therapy. Its objectives were: (1) to obtain feedback from participants and trainers on their experiences and acceptability of the REAP program and (2) to measure changes in vocational and cognitive skills of participants in the REAP program.

Materials and methods

This was a pretest–posttest mixed methods study. 15 adults with intellectual and developmental disabilities attending work therapy in a non-profit organization participated in the REAP program and their vocational trainers were involved in assisting in this program. Feasibility Evaluation Checklist (FEC) and the Neurobehavioral Cognitive Status Exam (Cognistat) were administered at baseline, post-training and eight weeks after training. The participants and their trainers also provided user feedback via semi-structured interviews.

Results

Majority of the participants and trainers found the REAP program to be useful and interesting. They also found that the augmented reality games were user-friendly and provided a unique opportunity to acquire new skills. Participants who engaged in this program showed a significant improvement in vocational skills and aspects of cognitive skills, which were maintained eight weeks after training.

Conclusion

The gamified augmented reality vocational training was feasible and accepted by both adults with intellectual and developmental disabilities and their trainers. When integrated with bridging sessions to facilitate transfer of learning to existing work therapy, participants on the REAP program showed significant improvements in vocational skills and aspects of cognitive skills. Future experimental studies with larger sample size could provide stronger evidence on its effectiveness in improving vocational outcomes.
Benefits and challenges of a personal budget for people with mental health conditions or intellectual disability: A systematic review
Authors: Micai Martina , Gila Letizia , Caruso Angela , Fulceri Francesca , Fontecedro Elisa , Castelpietra Giulio , Romano Giovanna , Ferri Mila , Scattoni Maria Luisa
Published: August 2022

Personal budgets (PBs) may improve the lives of people with mental health conditions and people with intellectual disability (ID). However, a clear definition of PB, benefits, and challenges is still faded. This work aims to systematically review evidence on PB use in mental health and ID contexts, from both a qualitative and quantitative perspective, and summarize the recent research on interventions, outcomes, and cost-effectiveness of PBs in beneficiaries with mental health conditions and/or ID. The present systematic review is an update of the existing literature analyzed since 2013. We performed a systematic search strategy of articles using the bibliographic databases PubMed and PsycINFO. Six blinded authors screened the works for inclusion/exclusion criteria, and two blinded authors extracted the data. We performed a formal narrative synthesis of the findings from the selected works. A total of 9,800 publications were screened, and 29 were included. Improvement in responsibility and awareness, quality of life, independent living, paid work, clinical, psychological, and social domains, and everyday aspects of the users’ and their carers’ life have been observed in people with mental health conditions and/or ID. However, the PBs need to be less stressful and burdensome in their management for users, carers, and professionals. In addition, more quantitative research is needed to inform PBs’ policymakers.

Systematic Review Registration

[www.crd.york.ac.uk/prospero/], identifier [CRD42020172607].
The need for new metrics in the Anthropocene era
Authors: Vineis Paolo , Mangone Lorenzo
Published: August 2022

A limitation in the discussion concerning climate change is the large degree of separation between scientific, economic, and technological approaches to tackle the crisis. This issue is most noticeable when considering the lack of metrics to measure the impact of different productive sectors on both the environment and the health of the population. The best-known attempt to measure these repercussions has been the introduction of the Environmental, Social and Governance (ESG) ratings for bonds. However, this rating system suffers from a lack of transparency and standardization. Moreover, it does not offer insights on the health impact and the regenerative effort of the evaluated bonds. Thus, we think it is necessary to introduce new metrics, focusing on at least four dimensions: circularity, climate change, biodiversity and health (including well-being). A sector that needs a special consideration is that of energy. To better compare different energy sources, we propose to adjust metrics such as the Energy Return on Investment (EROI) or the energy intensity metrics to include the negative health effects and the environmental degradation associated with producing energy. A similar index of return on investment corrected for health impacts may be considered to evaluate food production as well. Hyper-analytical and extremely focused approaches have dominated the discussion around the environmental crisis. We believe that a more inclusive approach is now needed, to highlight the potential co-benefits of different strategies, especially those that promote regeneration and a truly circular economy.
Machine learning model for depression based on heavy metals among aging people: A study with National Health and Nutrition Examination Survey 2017–2018
Authors: Xia Fang , Li Qingwen , Luo Xin , Wu Jinyi
Published: August 2022

Objective

To explore the association between depression and blood metal elements, we conducted this machine learning model fitting research.

Methods

Datasets from the National Health and Nutrition Examination Survey (NHANES) in 2017–2018 were downloaded (https://www.cdc.gov/nchs/nhanes). After screening, 3,247 aging samples with 10 different metals [lead (Pb), mercury (Hg), cadmium (Cd), manganese (Mn), selenium (Se), chromium (Cr), cobalt (Co), inorganic mercury (InHg), methylmercury (MeHg) and ethyl mercury (EtHg)] were included. Eight machine learning algorithms were compared for analyzing metal and depression. After comparison, XGBoost showed optimal effects. Poisson regression and XGBoost model (a kind of decision tree algorithm) were conducted to find the risk factors and prediction for depression.

Results

A total of 344 individuals out of 3247 participants were diagnosed with depression. In the Poisson model, we found Cd (β = 0.22, P = 0.00000941), EtHg (β = 3.43, P = 0.003216), and Hg (β=-0.15, P = 0.001524) were related with depression. XGBoost model was the suitable algorithm for the evaluation of depression, the accuracy was 0.89 with 95%CI (0.87, 0.92) and Kappa value was 0.006. Area under the curve (AUC) was 0.88. After that, an online XGBoost application for depression prediction was developed.

Conclusion

Blood heavy metals, especially Cd, EtHg, and Hg were significantly associated with depression and the prediction of depression was imperative.
The balancing act: Identifying multivariate sports performance using Pareto frontiers
Authors: Newans Tim , Bellinger Phillip , Minahan Clare
Published: August 2022

Athletes often require a mix of physical, physiological, psychological, and skill-based attributes that can be conflicting when competing at the highest level within their sport. When considering multiple variables in tandem, Pareto frontiers is a technique that can identify the observations that possess an optimal balance of the desired attributes, especially when these attributes are negatively correlated. This study presents Pareto frontiers as a tool to identify athletes who possess an optimal ranking when considering multiple metrics simultaneously. This study explores the trade-off relationship between batting average and strike rate as well as bowling strike rate, economy, and average in Twenty 20 cricket. Eight hundred ninety-one matches of Twenty 20 cricket from the men's (MBBL) and women's (WBBL) Australian Big Bash Leagues were compiled to determine the best batting and bowling performances, both within a single innings and across each player's Big Bash career. Pareto frontiers identified 12 and seven optimal batting innings performances in the MBBL and WBBL respectively, with nine and six optimal batting careers respectively. Pareto frontiers also identified three optimal bowling innings in both the MBBL and WBBL and five and six optimal bowling careers in MBBL and WBBL, respectively. Each frontier identified players that were not the highest ranked athlete in any metric when analyzed univariately. Pareto frontiers can be used when assessing talent across multiple metrics, especially when these metrics may be conflicting or uncorrelated. Using Pareto frontiers can identify athletes that may not have the highest ranking on a given metric but have an optimal balance across multiple metrics that are associated with success in a given sport.
The effect of phenobarbital treatment on behavioral comorbidities and on the composition and function of the fecal microbiome in dogs with idiopathic epilepsy
Authors: Watanangura Antja , Meller Sebastian , Suchodolski Jan S. , Pilla Rachel , Khattab Mohammad R. , Loderstedt Shenja , Becker Lisa F. , Bathen-Nöthen Andrea , Mazzuoli-Weber Gemma , Volk Holger A.
Published: August 2022

Phenobarbital (PB) is one of the most important antiseizure drugs (ASDs) to treat canine idiopathic epilepsy (IE). The effect of PB on the taxonomic changes in gastrointestinal microbiota (GIM) and their functions is less known, which may explain parts of its pharmacokinetic and pharmacodynamic properties, especially its antiseizure effect and drug responsiveness or drug resistance as well as its effect on behavioral comorbidities. Fecal samples of 12 dogs with IE were collected prior to the initiation of PB treatment and 90 days after oral PB treatment. The fecal samples were analyzed using shallow DNA shotgun sequencing, real-time polymerase chain reaction (qPCR)-based dysbiosis index (DI), and quantification of short-chain fatty acids (SCFAs). Behavioral comorbidities were evaluated using standardized online questionnaires, namely, a canine behavioral assessment and research questionnaire (cBARQ), canine cognitive dysfunction rating scale (CCDR), and an attention deficit hyperactivity disorder (ADHD) questionnaire. The results revealed no significant changes in alpha and beta diversity or in the DI, whereas only the abundance of Clostridiales was significantly decreased after PB treatment. Fecal SCFA measurement showed a significant increase in total fecal SCFA concentration and the concentrations of propionate and butyrate, while acetate concentrations revealed an upward trend after 90 days of treatment. In addition, the PB-Responder (PB-R) group had significantly higher butyrate levels compared to the PB-Non-Responder (PB-NR) group. Metagenomics of functional pathway genes demonstrated a significant increase in genes in trehalose biosynthesis, ribosomal synthesis, and gluconeogenesis, but a decrease in V-ATPase-related oxidative phosphorylation. For behavioral assessment, cBARQ analysis showed improvement in stranger-directed fear, non-social fear, and trainability, while there were no differences in ADHD-like behavior and canine cognitive dysfunction (CCD) scores after 90 days of PB treatment. While only very minor shifts in bacterial taxonomy were detected, the higher SCFA concentrations after PB treatment could be one of the key differences between PB-R and PB-NR. These results suggest functional changes in GIM in canine IE treatment.
In silico structural homology modeling and functional characterization of Mycoplasma gallisepticum variable lipoprotein hemagglutin proteins
Authors: Mugunthan Susithra Priyadarshni , Harish Mani Chandra
Published: August 2022

Mycoplasma gallisepticum variable lipoprotein hemagglutin (vlhA) proteins are crucial for immune evasion from the host cells, permitting the persistence and survival of the pathogen. However, the exact molecular mechanism behind the immune evasion function is still not clear. In silico physiochemical analysis, domain analysis, subcellular localization, and homology modeling studies have been carried out to predict the structural and functional properties of these proteins. The outcomes of this study provide significant preliminary data for understanding the immune evasion by vlhA proteins. In this study, we have reported the primary, secondary, and tertiary structural characteristics and subcellular localization, presence of the transmembrane helix and signal peptide, and functional characteristics of vlhA proteins from M. gallisepticum strain R low. The results show variation between the structural and functional components of the proteins, signifying the role and diverse molecular mechanisms in functioning of vlhA proteins in host immune evasion. Moreover the 3D structure predicted in this study will pave a way for understanding vlhA protein function and its interaction with other molecules to undergo immune evasion. This study forms the basis for future experimental studies improving our understanding in the molecular mechanisms used by vlhA proteins.
Barriers to Practicing COVID-19 Preventive Behaviors Among Migrant Workers in Qatar: A Qualitative Study During the First Wave of the Pandemic
Authors: Al-Jayyousi Ghadir Fakhri , Nour Mohamed , Suliman Jinan , Abed Alah Muna , Ali Khaled , Abdeen Sami , Al-Thani Mohammed , Jaffrey Shariq , Al-Romaihi Hamad Eid , Farag Elmoubasher
Published: August 2022

Objectives: Manual and Craft Workers (MACWs), who constitute more than 80% of the population, were identified to be a vulnerable group to COVID-19 in Qatar. The goal of this study is to identify the limitations face MACWs in Qatar towards practicing the COVID-19 preventive measures and thereby designing behavioral change strategies.

Methods: This is a qualitative research study in which individual interviews and focus group discussions were utilized for a deep understanding of the phenomenon from key informants. Four onlive individual interviews and four focus groups (n = 55) were conducted and transcribed verbatim. Inductive qualitative analysis was followed to discover the themes of the interviews. Data were analyzed using constant comparative techniques.

Results: Major themes elicited from the analysis revealed that the barriers to following COVID-19 preventive behaviors among migrant workers in Qatar included barriers related to knowledge and risk perception; lifestyle and habits; nature of work and living conditions, and barriers related to health communication, diversified cultures, and languages.

Conclusion: The findings would support constructing culturally sensitive health education messages and planning for effective health communication campaigns.