bluuebunny/crossref_metadata_2025 huggingface dataset:
Impulsivity, Social Support and Depression Are Associated With Latent Profiles of Internet Addiction Among Male College Freshmen
Authors: Zhang Yue , Liu Zhuang , Zhao Yuhong
Published: March 2021

Background: The rate of internet addiction is increasing in college students. The first year at college is a particularly vulnerable period for internet addiction. Students' psychological characteristics are likely to play an important role in internet addiction. Our study aimed to assess the relationship between impulsivity, social support, depression and internet addiction among male college freshmen.

Materials and Methods: The current study utilized latent profile analysis (LPA) to identify at-risk profiles among 734 college freshmen (100% male) based on their Internet Addiction Test item ratings. We compared the levels of impulsivity, social support and depression among different profiles and investigated whether these variables could predict each latent internet addiction class.

Results: LPA resulted in three distinct profiles: the low internet addiction group (42.10%), the moderate internet addiction group (35.70%) and the high internet addiction group (22.20%). Impulsivity and depression increased with internet addiction severity levels, whereas social support was inversely related to the severity of internet addiction. Male freshmen with high impulsivity, low social support and high depression were more likely to be included in the high internet addiction group.

Conclusion: This study highlights that impulsivity, social support and depression may predict internet addiction in male college freshmen. Our findings have important practical implications for college educators and counselors in developing interventions for internet addiction.
Analysis of Epidemiological and Clinical Characteristics of COVID-19 in Northwest Mexico and the Relationship Between the Influenza Vaccine and the Survival of Infected Patients
Authors: Angulo-Zamudio Uriel A. , Martínez-Villa Francisco M. , Leon-Sicairos Nidia , Flores-Villaseñor Hector , Velazquez-Roman Jorge , Campos-Romero Abraham , Alcántar-Fernández Jonathan , Urrea Francisco , Muro-Amador Secundino , Medina-Serrano Julio , Martinez-Garcia Jesus J. , Sanchez-Cuen Jaime , Angulo-Rocha Jorge , Canizalez-Roman Adrian
Published: March 2021

The first cases of unexplained pneumonia were reported in Wuhan, China, in December of 2019. Later, a novel coronavirus (SARS-CoV-2) was identified as the causal agent of pneumonia. This virus has since spread to more than 180 countries and has been declared a pandemic by the World Health Organization. Herein, we aimed to determine the epidemiological and clinical characteristics of symptomatic patients with coronavirus disease 2019 (COVID-19) and the relationship between the influenza vaccine with a lower risk of severe COVID-19 infection in the state of Sinaloa. We collected demographic and clinical data of 4,040 patients with acute respiratory infections across Sinaloa state hospitals from February 28 to May 15, 2020. The prevalence of COVID-19 among hospitalized patients with respiratory symptoms in Sinaloa showed 45.2% of men were more affected than women (p < 0.001), and people aged 40–49 years were the most affected. The main symptoms of COVID-19 infection were cough and fever (p < 0.001), while hypertension, obesity, and type 2 diabetes were the chronic diseases associated with COVID-19 than non-COVID-19 (p < 0.003). Healthcare workers were most likely to be infected compared to other occupations (p < 0.001). The general lethality rate was 14.1%, and males >62 years were the ones who had a higher lethality rate (p < 0.001); the aforementioned chronic diseases were related to higher lethality of COVID-19 (p < 0.001). Likewise, higher lethality was seen in housewives and patient retirees/pensioners compared with other occupations (p < 0.001). Finally, we found there was a relationship between influenza vaccination and a lower risk of severe COVID-19 infection and mortality (p < 0.001). These findings showed that healthcare workers, men >62 years with chronic diseases, and retired people were most affected. Furthermore, the influenza vaccine could decrease the severeness of COVID-19 cases.
From Autonomy to Integration, From Integration to Dynamically Balanced Integrated Co-existence: Non-aging as the Third Stage of Development
Authors: Salnikov Lev , Baramiya Mamuka G.
Published: March 2021

Reversible senescence at the cellular level emerged together with tissue specialization inMetazoans. However, this reversibility (ability to permanently rejuvenate) through recapitulation of early stages of development, was originally a part of ontogenesis, since the pressure of integrativeness was not dominant. The complication of specialization in phylogenesis narrowed this “freedom of maneuver”, gradually “truncating” remorphogenesis to local epimorphosis and further up to the complete disappearance of remorphogenesis from the ontogenesis repertoire. This evolutionary trend transformed cellular senescence into organismal aging and any recapitulation of autonomy into carcinogenesis. The crown of specialization,Homo sapiens, completed this post-unicellular stage of development, while in the genome all the potential for the next stage of development, which can be called the stage of balanced coexistence of autonomous and integrative dominants within a single whole. Here, completing the substantiation of the new section of developmental biology, we propose to call it Developmental Biogerontology.
Conformational Changes of the Receptor Binding Domain of SARS-CoV-2 Spike Protein and Prediction of a B-Cell Antigenic Epitope Using Structural Data
Authors: Khare Sangeeta , Azevedo Marli , Parajuli Pravin , Gokulan Kuppan
Published: March 2021

COVID-19, the illness caused by the SARS-CoV-2 virus, is now a worldwide pandemic with mortality in hundreds of thousands as infections continue to increase. Containing the spread of this viral infection and decreasing the mortality rate is a major challenge. Identifying appropriate antigenic epitopes from the viral proteins is a very important task for vaccine production and the development of diagnostic kits and antibody therapy. A novel antigenic epitope would be specific to the SARS-CoV-2 virus and can distinguish infections caused by common cold viruses. In this study two approaches are employed to identify both continuous and conformational B-cell antigenic epitopes. To achieve this goal, we modeled a complete structure of the receptor binding domain (RBD) of the spike protein using recently deposited coordinates (6vxx, 6vsb, and 6w41) in the protein data bank. In addition, we also modeled the RBD-ACE2 receptor complex for SARS-CoV-2 using the SARS-CoV RBD-ACE2 complex (3D0J) as a reference model. Finally, structure based predicted antigenic epitopes were compared to the ACE2 binding region of RBD of SARS-CoV-2. The identified conformational epitopes show overlaps with the ACE2-receptor binding region of the RBD of SARS-CoV-2. Strategies defined in the current study identified novel antigenic epitope that is specific to the SARS-CoV-2 virus. Integrating such approach in the diagnosis can distinguish infections caused by common cold viruses from SARS-CoV-2 virus.
Objectively Measured Physical Activity Increases Only in Males During a Summer Camp for Obese Children
Authors: Izzicupo Pascal , Di Blasio Andrea , Di Credico Andrea , Ghinassi Barbara , Capranica Laura , Napolitano Giorgio , Di Baldassarre Angela , Modestini Elisabetta , Di Pietro Mario
Published: March 2021

Childhood obesity is a major public health challenge. Summer camps for children with obesity represent an alternative setting to improve eating and physical activity habits. Here we evaluated if the participation in the camp improves objectively measured physical activity and sedentary behavior and whether there are differences between male and female participants. Twenty-eight children, 13 males and 15 females (body mass index >97° centile, weight excess >30%, Tanner stage I), agreed to participate in an 8-day camp. During the summer camp, children participated in sports-like games and outdoor activities for at least 3 h a day, and the school-camp staff also provided a theoretical nutritional learning plan. Accelerometry-derived physical activity was measured through the SenseWear Mini Armband during a week at home and during the camp experience. Before camping, the participants were far above the minimum daily values of moderate- to vigorous-intensity physical activity (MVPA) to be considered sufficiently active (≥60 min/day), but male participants were more active than females (MVPA: 186.2 ± 94.2, 111.0 ± 64.7; P = 0.020). Male participants increased their MVPA (234.3 ± 114.8, P = 0.020), whereas females not (111.9 ± 52.9, P = 0.020). No difference emerged for the sedentary behavior either before or during the camp. This study suggests that participation in a summer camp for obese children can determine different responses in physical activity levels, depending on the sex of young participants. Thus, summer camps for obese children should put particular attention on female participants, besides reducing sedentary behavior in both males and females.
Pediatric Challenges in Robot-Assisted Kidney Transplantation
Authors: Grammens Julien , Schechter Michal Yaela , Desender Liesbeth , Claeys Tom , Sinatti Céline , VandeWalle Johan , Vermassen Frank , Raes Ann , Vanpeteghem Caroline , Prytula Agnieszka , Silay Mesrur Selçuk , Breda Alberto , Decaestecker Karel , Spinoit Anne-Françoise
Published: March 2021

Kidney transplantation is universally recognized as the gold standard treatment in patients with End-stage Kidney Disease (ESKD, or according to the latest nomenclature, CKD stage 5). Robot-assisted kidney transplantation (RAKT) is gradually becoming preferred technique in adults, even if applied in very few centra, with potentially improved clinical outcomes compared with open kidney transplantation. To date, only very few RAKT procedures in children have been described. Kidney transplant recipient patients, being immunocompromised, might be at increased risk for perioperative surgical complications, which creates additional challenges in management. Applying techniques of minimally invasive surgery may contribute to the improvement of clinical outcomes for the pediatric transplant patients population and help mitigate the morbidity of KT. However, many challenges remain ahead. Minimally invasive surgery has been consistently shown to produce improved clinical outcomes as compared to open surgery equivalents. Robot-assisted laparoscopic surgery (RALS) has been able to overcome many restrictions of classical laparoscopy, particularly in complex and demanding surgical procedures. Despite the presence of these improvements, many challenges lie ahead in the surgical and technical–material realms, in addition to anesthetic and economic considerations. RALS in children poses additional challenges to both the surgical and anesthesiology team, due to specific characteristics such as a small abdominal cavity and a reduced circulating blood volume. Cost-effectiveness, esthetic and functional wound outcomes, minimal age and weight to undergo RALS and effect of RAKT on graft function are discussed. Although data on RAKT in children is scarce, it is a safe and feasible procedure and results in excellent graft function. It should only be performed by a RAKT team experienced in both RALS and transplantation surgery, fully supported by a pediatric nephrology and anesthesiology team. Further research is necessary to better determine the value of the robotic approach as compared to the laparoscopic and open approach. Cost-effectiveness will remain an important subject of debate and is in need of further evaluation as well.
Bovine Tuberculosis: The Emergence of a New Wildlife Maintenance Host in Ireland
Authors: Kelly David J. , Mullen Enda , Good Margaret
Published: March 2021

Despite advances in herd management, tuberculosis (TB) continues to affect ~0. 5% of Ireland's national cattle herd annually. It is clear that any “final” eradication of TB in cattle will need to address all TB maintenance hosts in the same environment. In Ireland and the UK, European Badgers (Meles meles) are a known TB maintenance host, while deer are recognised as spillover hosts. However, deer have been identified as maintenance hosts in other countries and Sika deer, specifically, have been identified with TB in Ireland. We examined the power of cattle, badger and Sika deer densities (at the county level) to predict cattle TB-breakdowns in Ireland, at both the herd and the individual level, using data collected between 2000 and 2018. Our hypothesis was that any positive correlations between deer density and cattle TB-breakdowns would implicate deer as TB maintenance hosts. Using linear multiple regressions, we found positive correlations between deer density and cattle TB-breakdowns at both the herd and individual levels. Since Sika deer in County Wicklow are known to have TB, we ran further regressions against subsets of data which excluded individual Irish counties. Analyses excluding Wicklow data showed much weaker correlations between Sika deer density and cattle TB-breakdowns at both the herd and individual levels, suggesting that these correlations are strongest in County Wicklow. A similar effect for badger density was seen in County Leitrim. While locally high densities of Sika deer persist in Irish counties, we believe they should be considered an integral part of any TB-control programme for those areas.
Cholinesterase is Associated With Prognosis and Response to Chemotherapy in Advanced Gastric Cancer
Authors: Bi Yanzhi , Zhang Junling , Zeng Dongxiang , Chen Lili , Ye Wei , Yang Quanliang , Ling Yang
Published: March 2021

Background: Cholinesterase (CHE) is a routine serum biomarker in gastric cancer (GC). However, little research has been done on its clinical value in advanced GC. In addition, it is not clear whether it can be used as biomarker for the response and prognosis of advanced GC patients.

Methods: Between Jan. 2013 and Dec. 2016, a total of 150 patients with advanced GC treated with first-line chemotherapy were admitted to Changzhou Tumor Hospital Affiliated to Soochow University. We retrospectively identified serum CHE level on the day before chemotherapy and at the end of chemotherapy and abstracted clinicopathologic features and treatment outcomes. Univariate and multivariate survival analyses were performed to assess the relationship between serum CHE levels and progression-free survival (PFS) and overall survival (OS).

Results: A total of 150 advanced GC patients were included and divided into serum level ≥5,000 IU/L and serum level <5,000 IU/L. CHE level lower than 5,000 IU/L was associated with poorer PFS (HR, 1.60; 95% CI, 1.141–2.243; p = 0.006), poorer OS (HR, 1.76; 95% CI, 1.228–2.515; p = 0.002) and trend of poorer response (HR, 0.56; 95% CI, 0.272–1.129; p = 0.104). In univariate and multivariate logistic regression analysis, only liver metastasis and PS score were significantly associated with objective response (p < 0.05). The medium PFS was 8.0 months in patients with post-treatment CHE increased vs. 3.8 months in patients with CHE decreased after chemotherapy (HR, 1.82; 95% CI 1.28–2.57; p = 0.0002). The medium OS was 13.1 months in patients with increased post-treatment CHE vs. 8.1 months in patients with decreased post-treatment CHE (HR, 1.87; 95% CI 1.29–2.71; p = 0.0002).

Conclusion: Advanced GC with CHE levels below 5,000 IU/L was significantly associated with poor PFS and OS. The results suggested that CHE analysis before chemotherapy was a promising prognostic marker for advanced GC.
A Prospective Study of Training Methods for Two-Year-Old Thoroughbred Racehorses in Queensland, Australia, and Analysis of the Differences in Training Methods between Trainers of Varying Stable Sizes
Authors: Crawford Kylie L. , Finnane Anna , Greer Ristan M. , Phillips Clive J. C. , Bishop Emma L. , Woldeyohannes Solomon M. , Perkins Nigel R. , Ahern Benjamin J.
Published: March 2021

Worldwide, musculoskeletal injuries remain a major problem for the Thoroughbred racing industry. There is a strong interest in developing training and management strategies to reduce the impact of musculoskeletal injuries, however, progress has been limited by studies reporting conflicting findings, and a limited understanding of the role of different training methods in preventing injury. There is little data on patterns of rest periods and exercise data and how these vary between trainers. This prospective study of two-year-old racehorses was conducted in Queensland, Australia and involved weekly personal structured interviews with 26 trainers over 56 weeks. Detailed daily exercise data for 535 horses providing 1258 training preparations and 7512 weeks at risk were collected. Trainers were categorised into three groups by the mean number of two-year-old horses that they had in work each week over the study duration: (1) Small stables with five or less, (2) Medium stables with 6 to 15 and (3) Large stables with greater than 15 horses in training. Differences between trainers with small, medium and large stable sizes were evaluated using linear regression, Kruskal–Wallis equality-of-populations rank test if linear models were mis-specified or Chi-squared tests for categorical variables. Significant differences were observed between trainers, with horses from larger stables accumulating a greater high-speed exercise volume (p < 0.001), attaining training milestones more frequently (p = 0.01) and taking less time to reach their training milestones (p = 0.001). This study provides detailed data to which training practices from other locations can be compared. Presenting actual training data rather than trainers’ estimation of a typical program provides a more accurate assessment of training practices. Understanding how training practices vary between regions improves comparability of studies investigating risk factors and is an important step towards reducing the impact of musculoskeletal injuries.
Microbial and Antimicrobial Resistance Profiles of Microbiota in Common Carps (Cyprinus carpio) from Aquacultured and Wild Fish Populations
Authors: Ruzauskas Modestas , Armalytė Julija , Lastauskienė Eglė , Šiugždinienė Rita , Klimienė Irena , Mockeliūnas Raimundas , Bartkienė Elena
Published: March 2021

In this study we analyzed differences in microbial composition and antimicrobial resistance profiles in common carp living in two different environments: fish ponds, where carp have been kept under the same growing conditions over the last 50 years, and from the wild. The results demonstrated that wild fish carry a great variety of bacterial species (448 species with a prevalence of at least 0.01% from the total number of reads). Aquacultured individuals harbored 2.56 times fewer species in their gut. Significant microbial differences were observed in all taxonomic ranks, including bacterial classes and phyla. Besides bacterial variety, it was determined that aquacultured fish harbored more bacteria that are considered pathogens or opportunistic pathogens, such as Moraxellaceae, Flavobacteriaceae, and Staphylococcaceae. The frequency of antimicrobial resistance in bacterial indicators was more common in aquacultured fish than in wild fish, therefore fish farming may be a potential source of environmental contamination with antimicrobial resistant bacteria.
The Usefulness of Mesenchymal Stem Cells beyond the Musculoskeletal System in Horses
Authors: Cequier Alina , Sanz Carmen , Rodellar Clementina , Barrachina Laura
Published: March 2021

The differentiation ability of mesenchymal stem cells (MSCs) initially raised interest for treating musculoskeletal injuries in horses, but MSC paracrine activity has widened their scope for inflammatory and immune-mediated pathologies in both equine and human medicine. Furthermore, the similar etiopathogenesis of some diseases in both species has advanced the concept of “One Medicine, One Health”. This article reviews the current knowledge on the use of MSCs for equine pathologies beyond the locomotor system, highlighting the value of the horse as translational model. Ophthalmologic and reproductive disorders are among the most studied for MSC application. Equine asthma, equine metabolic syndrome, and endotoxemia have been less explored but offer an interesting scenario for human translation. The use of MSCs in wounds also provides a potential model for humans because of the healing particularities in both species. High-burden equine-specific pathologies such as laminitis have been suggested to benefit from MSC-therapy, and MSC application in challenging disorders such as neurologic conditions has been proposed. The available data are preliminary, however, and require further development to translate results into the clinic. Nevertheless, current evidence indicates a significant potential of equine MSCs to enlarge their range of application, with particular interest in pathologies analogous to human conditions.
The Effect of Infertility on the Liver Structure, Endocrinology, and Gene Network in Japanese Flounder
Authors: Mang Qi , Hou Jilun , Han Tian , Wang Guixing , Wang Yufen , Liu Yufeng , Ren Yuqin , Zhao Yaxian , He Zhongwei , Zhang Xiaoyan
Published: March 2021

The liver can synthesize vitellogenin, the precursor of vitellin, which is needed for oocyte development and maturation. Here, we investigated the effects of infertility on liver structure, hormone regulation, and gene and protein networks in Japanese flounder (Paralichthys olivaceus). Results showed that the liver of infertile fish had fewer vacuoles and significantly lower serum vitellogenin (VTG) level than in liver of fertile fish. Whole transcriptomics analysis between infertile and fertile groups identified 2076 significantly differentially expressed (DE) mRNAs, 431 DE lncRNAs, 265 DE circRNAs, and 53 DE miRNAs. Proteomics analysis identified 838 DE proteins. Integrated analysis of whole transcriptomics and proteomics revealed 60 significantly DE genes and proteins associated with metabolism, immunity, signal transduction, and steroid biosynthesis. Moreover, non-coding RNA (miRNAs, circRNA, and lncRNA) transcripts involved in metabolism, immunity, and signal transduction in infertile liver were identified. In conclusion, this study shows that gonadal infertility is associated with not only changes in histological structure and hormone secretion but also changes in metabolism, immunity, and signal transduction networks in the liver. These results provide valuable information concerning the mechanism underlying infertility in fish.
Target Attainment and Clinical Efficacy for Vancomycin in Neonates: Systematic Review
Authors: Mejías-Trueba Marta , Alonso-Moreno Marta , Herrera-Hidalgo Laura , Gil-Navarro Maria Victoria
Published: March 2021

Vancomycin is commonly used as a treatment for neonatal infections. However, there is a lack of consensus establishing the optimal vancomycin therapeutic regimen and defining the most appropriate PK/PD parameter correlated with the efficacy. A recent guideline recommends AUC–guided therapeutic dosing in treating serious infections in neonates. However, in clinical practice, trough serum concentrations are commonly used as a surrogate PKPD index for AUC24. Despite this, target serum concentrations in a neonatal population remain poorly defined. The objective is to describe the relationship between therapeutic regimens and the achievement of clinical or pharmacokinetic outcomes in the neonatal population. The review was carried out following PRISMA guidelines. A bibliographic search was manually performed for studies published on PubMed and EMBASE. Clinical efficacy and/or target attainment and the safety of vancomycin treatment were evaluated through obtaining serum concentrations. A total of 476 articles were identified, of which 20 met the inclusion criteria. All of them evaluated the target attainment, but only two assessed the clinical efficacy. The enormous variability concerning target serum concentrations is noteworthy, which translates into a difficulty in determining which therapeutic regimen achieves the best results. Moreover, there are few studies that analyze clinical efficacy results obtained after reaching predefined trough serum concentrations, this information being essential for clinical practice.
Efficacy of Toluidine Blue—Mediated Antimicrobial Photodynamic Therapy on Candida spp. A Systematic Review
Authors: Wiench Rafał , Skaba Dariusz , Matys Jacek , Grzech-Leśniak Kinga
Published: March 2021

The effectiveness of antimicrobial photodynamic therapy (aPDT) in the treatment of oral yeast infections was examined many times in recent years. The authors of this review tried to address the question: “Should TBO (toluidine blue ortho)-mediated aPDT be considered a possible alternative treatment for oral candidiasis?”. PubMed/Medline and the Cochrane Central Register of Controlled Trials (CEN-TRAL) databases were searched from 1997 up to the 27th of October 2020 using a combination of the following keywords: (Candida OR Candidiasis oral OR Candidosis oral OR denture stomatitis) AND (toluidine blue OR photodynamic therapy OR aPDT OR photodynamic antimicrobial chemotherapy OR PACT OR photodynamic inactivation OR PDI). Animal studies or in vitro studies involving Candida albicans (C. albicans) and/or nonalbicans stain, randomized clinical trials (RCT) involving patients with oral candidiasis or denture stomatitis published solely in English language were included. Candida elimination method in animal, in vitro studies and RCT used was TBO-mediated aPDT. Exactly 393 studies were taken into consideration. Then, after analyzing titles and abstracts of said studies, 361 were excluded. Only 32 studies ended up being selected for in-depth screening, after which 21 of them were included in this study. All studies reported the antifungal effectiveness of aPDT with TBO against C. albicans and non-albicans Candida. In studies conducted with planktonic cells, only one study showed eradication of C. albicans. All others showed partial elimination and only one of them was not statistically significant. Experiments on yeast biofilms, in all cases, showed partial, statistically significant cell growth inhibition and weight reduction (a reduction in the number of cells—mainly hyphae) and the mass of extracellular polymeric substance (EPS). In vivo aPDT mediated by TBO exhibits antifungal effects against oral Candida spp.; however, its clinical effectiveness as a potent therapeutic strategy for oral yeast infections requires further investigation.
Crosstalk between Brassinosteroid and Redox Signaling Contributes to the Activation of CBF Expression during Cold Responses in Tomato
Authors: Fang Pingping , Wang Yu , Wang Mengqi , Wang Feng , Chi Cheng , Zhou Yanhong , Zhou Jie , Shi Kai , Xia Xiaojian , Foyer Christine Helen , Yu Jingquan
Published: March 2021

Brassinosteroids (BRs) play a critical role in plant responses to stress. However, the interplay of BRs and reactive oxygen species signaling in cold stress responses remains unclear. Here, we demonstrate that a partial loss of function in the BR biosynthesis gene DWARF resulted in lower whilst overexpression of DWARF led to increased levels of C-REPEAT BINDING FACTOR (CBF) transcripts. Exposure to cold stress increased BR synthesis and led to an accumulation of brassinazole-resistant 1 (BZR1), a central component of BR signaling. Mutation of BZR1 compromised the cold- and BR-dependent increases in CBFs and RESPIRATORY BURST OXIDASE HOMOLOG 1(RBOH1) transcripts, as well as preventing hydrogen peroxide (H2O2) accumulation in the apoplast. Cold- and BR-induced BZR1 bound to the promoters of CBF1, CBF3 and RBOH1 and promoted their expression. Significantly, suppression of RBOH1 expression compromised cold- and BR-induced accumulation of BZR1 and related increases in CBF transcripts. Moreover, RBOH1-dependent H2O2 production regulated BZR1 accumulation and the levels of CBF transcripts by influencing glutathione homeostasis. Taken together, these results demonstrate that crosstalk between BZR1 and reactive oxygen species mediates cold- and BR-activated CBF expression, leading to cold tolerance in tomato (Solanum lycopersicum).
Efficacy of Alpinumisoflavone Isolated from Maclura tricuspidata Fruit in Tumor Necrosis Factor-α-Induced Damage of Human Dermal Fibroblasts
Authors: Lee Sullim , Hoang Giang Do , Kim Daeyoung , Song Ho Sueb , Choi Sungyoul , Lee Dongho , Kang Ki Sung
Published: March 2021

The skin is an important organ in the human body that protects the body from environmentally hazardous substances. Reactive oxygen species (ROS) cause inflammatory reactions and degradation of the extracellular matrix leading to skin aging and various cutaneous lesions. This study evaluated the potential of isoflavones isolated from Maclura tricuspidata fruit to prevent TNF-α-induced skin inflammation in normal human dermal fibroblasts (HDFs). It focused on alpinumisoflavone (AIF) that suppressed the accumulation of ROS and nitric oxide (NO) in tumor necrosis factor-alpha (TNF-α)-treated HDFs. AIF inhibited the TNF-α-induced increase in matrix metalloproteinase-1, decreased procollagen I α1, and suppressed pro-inflammatory mediators and pro-inflammatory cytokines, including NO synthase, cyclooxygenase-2, interleukin (IL)-1β, IL-6, and IL-8 that trigger inflammatory responses. AIF inhibited nuclear factor-κB and activating protein 1 mitogen-activated protein kinases that were increased by TNF-α stimulation. These results suggest that AIF may protect skin from aging and various cutaneous lesions.
Mathematical Modeling of Lactobacillus paracasei CBA L74 Growth during Rice Flour Fermentation Performed with and without pH Control
Authors: Colucci Cante Rosa , Gallo Marianna , Nigro Federica , Passannanti Francesca , Budelli Andrea , Nigro Roberto
Published: March 2021

The mathematical modeling of fermentation processes allows for the formulation of predictions about the kinetics of biomass growth and metabolite production as well as setting or verifying the best operative conditions in view of the economical convenience of the process. For this purpose, we performed a kinetic study of a rice flour fermentation process using Lactobacillus paracasei CBA L74 with and without pH control; the pH value was set to 5.8 under pH control. Monod, Logistic, and Contois models were proposed to describe the bacterial growth rate in both conditions. The best mathematical model, which was able to describe the experimental data obtained without pH control, was the Contois model, as the specific growth rate was influenced by both the glucose reduction (from 14.31 g/L to 10.22 g/L) and the biomass production (2 log growth) that occurred during fermentation. Conversely, when pH control was implemented, both Monod and Contois models satisfactorily described the specific growth rate trend. The estimated kinetic parameters confirmed that biomass production (2 log growth) and glucose consumption (from 14.31 g/L to 6.06 g/L) did not affect the microorganism’s growth capacity when the fermenting medium was maintained at an optimal pH. The lactic acid production rate described by the Luedeking–Piret model did not appear to be linked to growth in the absence of pH control while, on the other hand, this model was unsuitable for describing the experimental lactic acid concentration when pH control was applied. The kinetic modeling of lactic acid production and the percentage of added glucose in the protocol with controlled pH will be optimized in the future.
Object Detection, Distributed Cloud Computing and Parallelization Techniques for Autonomous Driving Systems
Authors: Cortés Gallardo Medina Edgar , Velazquez Espitia Victor Miguel , Chípuli Silva Daniela , Fernández Ruiz de las Cuevas Sebastián , Palacios Hirata Marco , Zhu Chen Alfredo , González González José Ángel , Bustamante-Bello Rogelio , Moreno-García Carlos Francisco
Published: March 2021

Autonomous vehicles are increasingly becoming a necessary trend towards building the smart cities of the future. Numerous proposals have been presented in recent years to tackle particular aspects of the working pipeline towards creating a functional end-to-end system, such as object detection, tracking, path planning, sentiment or intent detection, amongst others. Nevertheless, few efforts have been made to systematically compile all of these systems into a single proposal that also considers the real challenges these systems will have on the road, such as real-time computation, hardware capabilities, etc. This paper reviews the latest techniques towards creating our own end-to-end autonomous vehicle system, considering the state-of-the-art methods on object detection, and the possible incorporation of distributed systems and parallelization to deploy these methods. Our findings show that while techniques such as convolutional neural networks, recurrent neural networks, and long short-term memory can effectively handle the initial detection and path planning tasks, more efforts are required to implement cloud computing to reduce the computational time that these methods demand. Additionally, we have mapped different strategies to handle the parallelization task, both within and between the networks.
Effect of the Interfacial Dzyaloshinskii–Moriya Interaction on the Spin Waves Eigenmodes of Isolated Stripes and Dots Magnetized In-Plane: A Micromagnetic Study
Authors: Silvani Raffaele , Alunni Michele , Tacchi Silvia , Carlotti Giovanni
Published: March 2021

The influence of the Dzyaloshinskii–Moriya interaction (DMI) on the eigenmodes of magnetic nanostructures is attracting interest for both fundamental reasons and prospects in applications. In this study, the characteristics of spin waves eigenmodes in either long stripes or elliptical dots magnetized in-plane, with lateral dimensions of the order of 100 nm, are analyzed by micromagnetic simulations in presence of a sizeable DMI. Using the GPU-accelerated software MuMax3, we show that the eigenmodes spectrum is appreciably modified by the DMI-induced non-reciprocity in spin-waves propagation: the frequencies of the eigenmodes are red-shifted and their spatial profiles appreciable altered due to the lack of stationary character in the direction orthogonal to the magnetization direction. As a consequence, one finds a modification of the expected cross-section of the different modes in either ferromagnetic resonance or Brillouin light scattering experiments, enabling one to detect modes that would remain invisible without DMI. In this respect, the modifications of the spectrum can be directly connected to a quantitative estimation of the DMI constant. Moreover, it is seen that for sufficiently large values of the DMI constant, the low-frequency odd eigenmode changes its profile and becomes soft, reflecting the transition of the ground state from uniform to chiral.
Bone Marrow Aspirate Concentrate Is More Effective Than Hyaluronic Acid and Autologous Conditioned Serum in the Treatment of Knee Osteoarthritis: A Retrospective Study of 505 Consecutive Patients
Authors: Hussein Mohsen , van Eck Carola F. , Kregar Velikonja Nevenka
Published: March 2021

The aim of this study was to evaluate and compare the effectiveness of three different intra-articular injective treatments: hyaluronic acid (HA), autologous conditioned serum (ACS) and bone marrow aspirate concentrate (BMAC) for the treatment of knee osteoarthritis (OA). A Level III retrospective comparative clinical study was performed on 505 consecutive patients treated with HA (n = 171), ACS (n = 222) or BMAC (n = 112) for knee OA. The mean patient age was 52 ± 13 years; 54.5% were males. Collected data included patient demographics, symptoms, visual analogue scale (VAS), Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) and radiographic classification of osteoarthritis grade using plain radiographs and advanced imaging. Clinical outcome was assessed at 3 and 12 months post treatment. Significant improvement in VAS and WOMAC was seen for all three treatments at the 3-month follow-up. At 12 months, VAS was improved in all three treatment groups, yet only BMAC sustained the improved WOMAC even in patients with more severe degenerative changes. This study shows that BMAC is more effective than HA and ACS in the treatment of symptomatic knee OA, especially in the patients with more severe degenerative changes.
Aeroacoustics Assessment of an Hybrid Aircraft Configuration with Rear-Mounted Boundary Layer Ingested Engine
Authors: Petrosino Francesco , Barbarino Mattia , Staggat Martin
Published: March 2021

Hybrid electric propulsion is a promising solution to reduce aircraft emissions, thus improving the sustainability of the air transport. In this work, a hybrid aircraft configuration with a rear-mounted boundary layer ingestion (BLI) engine has been investigated. The partial embedding of the engine into the fuselage generates a distortion of the ingested inflow causing additional tonal and broadband BLI noise sources, and, at the same time, alters the existing one, such as the rotor–stator interaction noise (RSI). This work is focused on the tonal RSI noise modeling, with and without the distortion generated by the BLI, and the far-field propagation including the acoustic masking contribution due to the engine–fuselage integration. As the main result, this work shows the contributions of BLI and the engine–aircraft integration on the RSI noise. Both effects should be properly taken into account in the early aircraft design stage for an effective noise reduction even at ground level.
Effects of Adding Moringa oleifera Leaves Powder on the Nutritional Properties, Lipid Oxidation and Microbial Growth in Ground Beef during Cold Storage
Authors: Mashau Mpho Edward , Ramatsetse Kgaogelo Edwin , Ramashia Shonisani Eugenia
Published: March 2021

The utilisation of Moringa oleifera leaves powder (MOLP) to improve the nutritional properties and inhibit lipid oxidation and the proliferation of microorganisms in ground beef during cold storage was examined. The effects of 0.2, 0.4, 0.6, and 0.8% MOLP on the nutritional properties (proximate composition, total phenolic and total flavonoid content), thiobarbituric acid reactive substances (TBARS), microbial composition, physicochemical characteristics (pH value, colour attributes, and cooking properties), and sensory analysis of ground beef were investigated. The findings showed that ash, protein, polyphenolic compounds, pH, colour, and microbial growth increased significantly, while moisture, fat content, and TBARS decreased significantly, with an increase in the concentration of MOLP during cold storage. Moderate levels (0.2 and 0.4%) of MOLP did not affect the sensory attributes of stored ground beef. Evidently, MOLP can be utilised as a natural preservative in ground beef to improve the nutritional value and inhibit lipid oxidation.
Dynamic Balance of the Head in a Flexible Legged Robot for Efficient Biped Locomotion
Authors: Lisitano Domenico , Bonisoli Elvio , Recchiuto Carmine Tommaso , Muscolo Giovanni Gerardo
Published: March 2021

In the biped robotics domain, head oscillations may be extremely harmful, especially if the robot is teleoperated, since vibrations strongly reduce the operator’s spatial awareness. In particular, undesired head oscillations occur in under-actuated robots, where springs and passive mechanisms are used to achieve a human-like motion. This paper proposes an approach to reduce the vibrations of a biped robot’s head; the proposed solution does not affect the dynamic locomotion properties, on which specific control logic could have been already tuned. The approach is tested on Rollo, a flexible-biped-wheeled robot, whose head vibrates throughout the robot locomotion. The two requirements, i.e., head vibration reduction and unchanged Rollo locomotion properties, are traduced in constraints to the robot possible modifications. Based on a 1D finite element model of the robot, tuned on experimental modal analysis, the undesired vibration causes are detected, and a solution for their reduction is proposed. Rollo’s head vibration amplitude is attenuated using a tuned vibration absorber, which achieves impressive performance in the robot. An archetype of the proposed vibration absorber is tailored designed on Rollo, without invasive changes to the robot structure. The proposed approach solves a significant problem in the biped robotic research community. The approach used to reduce the Rollo head oscillations may be utilized in other biped robot machines with or without flexible legs.
Generating DNA Origami Nanostructures through Shape Annealing
Authors: Babatunde Bolutito , Arias D. Sebastian , Cagan Jonathan , Taylor Rebecca E.
Published: March 2021

Structural DNA nanotechnology involves the design and self-assembly of DNA-based nanostructures. As a field, it has progressed at an exponential rate over recent years. The demand for unique DNA origami nanostructures has driven the development of design tools, but current CAD tools for structural DNA nanotechnology are limited by requiring users to fully conceptualize a design for implementation. This article introduces a novel formal approach for routing the single-stranded scaffold DNA that defines the shape of DNA origami nanostructures. This approach for automated scaffold routing broadens the design space and generates complex multilayer DNA origami designs in an optimally driven way, based on a set of constraints and desired features. This technique computes unique designs of DNA origami assemblies by utilizing shape annealing, which is an integration of shape grammars and the simulated annealing algorithm. The results presented in this article illustrate the potential of the technique to code desired features into DNA nanostructures.
Damage Detection of Carbon Nanotube Cementitious Composites Using Thermal and Electrical Resistance Properties
Authors: Lee Heeyoung , Yu Wonjun , Chung Wonseok
Published: March 2021

This study aimed to detect damage based on thermal and electrical resistance properties by fabricating composites in which multi-walled carbon nanotubes (MWCNTs) and cement-based materials are mixed. The experimental parameters used were the cement-based material type, MWCNT concentration, curing period, and presence of damage. The experimental results showed that damage in cement paste can be detected using the heat property at every MWCNT concentration, and damage in mortar can be detected at MWCNT concentrations of ≤0.25 wt%. However, damage to concrete is difficult to detect using the heat property. Damage to cement paste, mortar, and concrete can be detected at every concentration using the electrical resistance property. Furthermore, field emission scanning electron microscopy (FE-SEM) results revealed uniformly dispersed MWCNTs inside the composites without agglomeration or the formation of carbon nanotube (CNT) networks.
Role of Functional Monomers upon the Properties of Bisphenol A Molecularly Imprinted Silica Films
Authors: Gavrila Ana-Mihaela , Radu Ionut-Cristian , Stroescu Hermine , Zaharia Anamaria , Stoica Elena-Bianca , Ciurlica Ana-Lorena , Iordache Tanţa-Verona , Sârbu Andrei
Published: March 2021

In this study, two types of bisphenol A molecularly imprinted films (BPA-MIP) were successfully prepared via sol-gel derived methods using two different organosilane functional monomers N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (DAMO-T) or (3-mercaptopropyl)trimethoxysilane (MPTES). The physical-chemical characterization of films, in terms of morphology, structure, thermal analysis, and optical features, suggested that thinner films with a homogenous porous structure were more likely to retain BPA molecules. The MIP films revealed the rapid and quantitative adsorption of BPA, registering the most specific binding in the first five minutes of contact with the BPA-MIP film. Silica films were effectively regenerated for further usage for at least five times, demonstrating their high stability and reusability. Even if the performance of films for BPA uptake dropped dramatically after the third adsorption/reconditioning cycle, this synthesis method for BPA-MIP films has proven to be a reliable and cheap way to prepare sensitive films with potential application for re-usable optical sensors.
A Proposed Protocol for Ordinary and Extraordinary Hygienic Maintenance in Different Implant Prosthetic Scenarios
Authors: Felice Pietro , Bertacci Angelica , Bonifazi Lorenzo , Karaban Maryia , Canullo Luigi , Pistilli Roberto , Sammartino Pasquale , Gasparro Roberta , Barausse Carlo
Published: March 2021

Prevention of peri-implantitis involves the early diagnosis of peri-implant mucositis. This article presents a protocol of hygienic maintenance in different implant prosthetic scenarios: single crown, fixed partial prosthesis, fixed full-arch, and overdentures. Others clinical conditions have to be taken into consideration: patient compliance; history of periodontitis; implants placed in augmented bone; short, zygomatic, pterygoid, and tilted implants; and complex prosthesis with false gingiva. Two levels of implant maintenance are described: ordinary, performed by dental hygienist, and extraordinary, carried out by both dentist and hygienist. Extraordinary maintenance also involves the removal and decontamination of the prosthetic structure. To obtain an effective prevention of peri-implantitis, one must plan ordinary and extraordinary hygiene in relation to the type of rehabilitation and clinical parameters.
Monitoring Air Pollution Variability during Disasters
Authors: Nance Earthea
Published: March 2021

National environmental regulations lack short-term standards for variability in fine particulate matter (PM2.5); they depend solely on concentration-based standards. Twenty-five years of research has linked short-term PM2.5, that is, increases of at least 10 μg/m3 that can occur in-between regulatory readings, to increased mortality. Even as new technologies have emerged that could readily monitor short-term PM2.5, such as real-time monitoring and mobile monitoring, their primary application has been for research, not for air quality management. The Gulf oil spill offers a strategic setting in which regulatory monitoring, computer modeling, and stationary monitoring could be directly compared to mobile monitoring. Mobile monitoring was found to best capture the variability of PM2.5 during the disaster. The research also found that each short-term increase (≥10 μg/m3) in fine particulate matter was associated with a statistically significant increase of 0.105 deaths (p < 0.001) in people aged 65 and over, which represents a 0.32% increase. This research contributes to understanding the effects of PM2.5 on mortality during a disaster and provides justification for environmental managers to monitor PM2.5 variability, not only hourly averages of PM2.5 concentration.
Infarct in the Heart: What’s MMP-9 Got to Do with It?
Authors: Becirovic-Agic Mediha , Chalise Upendra , Daseke Michael J. , Konfrst Shelby , Salomon Jeffrey D. , Mishra Paras K. , Lindsey Merry L.
Published: March 2021

Over the past three decades, numerous studies have shown a strong connection between matrix metalloproteinase 9 (MMP-9) levels and myocardial infarction (MI) mortality and left ventricle remodeling and dysfunction. Despite this fact, clinical trials using MMP-9 inhibitors have been disappointing. This review focuses on the roles of MMP-9 in MI wound healing. Infiltrating leukocytes, cardiomyocytes, fibroblasts, and endothelial cells secrete MMP-9 during all phases of cardiac repair. MMP-9 both exacerbates the inflammatory response and aids in inflammation resolution by stimulating the pro-inflammatory to reparative cell transition. In addition, MMP-9 has a dual effect on neovascularization and prevents an overly stiff scar. Here, we review the complex role of MMP-9 in cardiac wound healing, and highlight the importance of targeting MMP-9 only for its detrimental actions. Therefore, delineating signaling pathways downstream of MMP-9 is critical.