bluuebunny/crossref_metadata_2025 huggingface dataset:
Urinary Incontinence in Women: Modern Methods of Physiotherapy as a Support for Surgical Treatment or Independent Therapy
Authors: Mazur-Bialy Agnieszka Irena , Kołomańska-Bogucka Daria , Nowakowski Caroline , Tim Sabina
Published: April 2020

Urinary incontinence (UI) is a common health problem affecting quality of life of nearly 420 million people, both women and men. Pelvic floor muscle (PFM) training and other physiotherapy techniques play an important role in non-surgical UI treatment, but their therapeutic effectiveness is limited to slight or moderate severity of UI. Higher UI severity requires surgical procedures with pre- and post-operative physiotherapy. Given that nearly 30%–40% of women without dysfunction and about 70% with pelvic floor dysfunction are unable to perform a correct PFM contraction, therefore, it is particularly important to implement physiotherapeutic techniques aimed at early activation of PFM. Presently, UI physiotherapy focuses primarily on PFM therapy and its proper cooperation with synergistic muscles, the respiratory diaphragm, and correction of improper everyday habits for better pelvic organ support and continence. The purpose of this work is a systematic review showing the possibilities of using physiotherapeutic techniques in the treatment of UI in women with attention to the techniques of PFM activation. Evidence of the effectiveness of well-known (e.g., PFM training, biofeedback, and electrostimulation) and less-known (e.g., magnetostimulation, vibration training) techniques will be presented here regarding the treatment of symptoms of urinary incontinence in women.
Novel MAG Variant Causes Cerebellar Ataxia with Oculomotor Apraxia: Molecular Basis and Expanded Clinical Phenotype
Authors: Santos Mariana , Damásio Joana , Kun-Rodrigues Célia , Barbot Clara , Sequeiros Jorge , Brás José , Alonso Isabel , Guerreiro Rita
Published: April 2020

Homozygous variants in MAG, encoding myelin-associated glycoprotein (MAG), have been associated with complicated forms of hereditary spastic paraplegia (HSP). MAG is a glycoprotein member of the immunoglobulin superfamily, expressed by myelination cells. In this study, we identified a novel homozygous missense variant in MAG (c.124T>C; p.Cys42Arg) in a Portuguese family with early-onset autosomal recessive cerebellar ataxia with neuropathy and oculomotor apraxia. We used homozygosity mapping and exome sequencing to identify the MAG variant, and cellular studies to confirm its detrimental effect. Our results showed that this variant reduces protein stability and impairs the post-translational processing (N-linked glycosylation) and subcellular localization of MAG, thereby associating a loss of protein function with the phenotype. Therefore, MAG variants should be considered in the diagnosis of hereditary cerebellar ataxia with oculomotor apraxia, in addition to spastic paraplegia.
Decreased Glucose Utilization Contributes to Memory Impairment in Patients with Glufosinate Ammonium Intoxication
Authors: Park Samel , Kim Joong Il , Cho Nam-jun , Oh Se Won , Park Jongkyu , Yoo Ik Dong , Gil Hyo-Wook , Lee Sang Mi
Published: April 2020

The symptoms of glufosinate ammonium (GLA) intoxication include gastrointestinal and neurologic symptoms, respiratory failure, and cardiovascular instability. Among these, neurologic symptoms including loss of consciousness, memory impairment, and seizure are characteristic of GLA poisoning. However, the mechanism of brain injury by GLA poisoning is still poorly understood. We investigated nine patients who had performed an F-18 fluorodeoxyglucose (FDG) positron emission tomography (PET) scan because of memory impairment caused by GLA ingestion. FDG-PET images of patients with GLA intoxication were compared with 24 age- and sex-matched healthy controls to evaluate whether the patients had abnormal patterns of glucose metabolism in the brain. Decreased glucose metabolism was observed in the inferior frontal and temporal lobes of these patients with GLA intoxication when compared with 24 age- and sex-matched healthy controls. Three patients performed follow-up FDG-PET scans. However, it was shown that the results of the follow-up FDG-PET scans were determined to be inconclusive. Our study showed that memory impairment induced by GLA intoxication was associated with glucose hypometabolism in the inferior frontal and temporal lobes in the brain.
Common Musculoskeletal Disorders in the Elderly: The Star Triad
Authors: Minetto Marco Alessandro , Giannini Alessandro , McConnell Rebecca , Busso Chiara , Torre Guglielmo , Massazza Giuseppe
Published: April 2020

Musculoskeletal disorders are debilitating conditions that significantly impair the state of health, especially in elderly subjects. A pathological triad of inter-related disorders that are highly prevalent in the elderly consists of the following main “components”: sarcopenia, tendinopathies, and arthritis. The aim of this review is to critically appraise the literature relative to the different disorders of this triad, in order to highlight the pathophysiological common denominator and propose strategies for personalized clinical management of patients presenting with this combination of musculoskeletal disorders. Their pathophysiological common denominator is represented by progressive loss of (focal or generalized) neuromuscular performance with a risk of adverse outcomes such as pain, mobility disorders, increased risk of falls and fractures, and impaired ability or disability to perform activities of daily living. The precise management of these disorders requires not only the use of available tools and recently proposed operational definitions, but also the development of new tools and approaches for prediction, diagnosis, monitoring, and prognosis of the three disorders and their combination.
Analysing Arbitrary Curves from the Line Hough Transform
Authors: Bailey Donald , Chang Yuan , Le Moan Steven
Published: April 2020

The Hough transform is commonly used for detecting linear features within an image. A line is mapped to a peak within parameter space corresponding to the parameters of the line. By analysing the shape of the peak, or peak locus, within parameter space, it is possible to also use the line Hough transform to detect or analyse arbitrary (non-parametric) curves. It is shown that there is a one-to-one relationship between the curve in image space, and the peak locus in parameter space, enabling the complete curve to be reconstructed from its peak locus. In this paper, we determine the patterns of the peak locus for closed curves (including circles and ellipses), linear segments, inflection points, and corners. It is demonstrated that the curve shape can be simplified by ignoring parts of the peak locus. One such simplification is to derive the convex hull of shapes directly from the representation within the Hough transform. It is also demonstrated that the parameters of elliptical blobs can be measured directly from the Hough transform.
Wave Breaker Types on a Smooth and Impermeable 1:10 Slope
Authors: Moragues María Victoria , Clavero María , Losada Miguel Á.
Published: April 2020

This research identified the types of wave breaker on a non-overtoppable, smooth and impermeable 1:10 slope under regular waves. Experimental tests were carried out in the Atmosphere-Ocean Interaction Flume of the Andalusian Institute for Earth System Research (University of Granada). Using the experimental space [log(h/L)–log(H/L)] and the alternate slope similarity parameter [χ = log (h/L H/L)], a complete set of breaker types was identified. Four types of wave breaker were then added to Galvin’s classification. Our results showed that the value of the Iribarren number was not sufficient to predict the expected type of wave breaker on the slope. Except for spilling and early plunging breakers, no biunivocal relationship was found between Ir and the type of breaker. The data obtained in the physical model were further enriched with the results of the flow characteristics and the wave energy transformation coefficients obtained with the IH-2VOF numerical model on a 1:10 impermeable slope. This research study, presented in this paper, showed that the Iribarren number is not a convenient wave breaking similarity parameter.
Crowdfunding: An Exploratory Study on Knowledge, Benefits and Barriers Perceived by Young Potential Entrepreneurs
Authors: Bernardino Susana , Santos J. Freitas
Published: April 2020

Crowdfunding (CF) has experienced impressive growth in recent years with the development of internet and information technologies that increased the participation of the “crowd” to fund entrepreneurial projects. Young entrepreneurs, especially well-qualified students, have recently begun to play a new role in the economy by launching new ventures in niche markets. The aim of the present paper is to provide a deeper understanding of CF among Portuguese young potential entrepreneurs as an alternative funding mechanism, by discussing its main characteristics and the perceived benefits and barriers that might drive young entrepreneurs to post a project on a CF platform or discourage its use. Through an online survey, we query well-qualified students about the knowledge they have about crowdfunding and benefits and barriers that can increase or reduce the possibility of funding to launch a new venture. The results show that potential young entrepreneurs have moderate knowledge about CF. Consequently, they are not able to explore all the business models available, specifically the models related to investment (lending and equity). The respondents perceive several benefits of the use of CF that go beyond the financial advantages, such as the communication of the project to a wider audience and the additional feedback from potential customers. The perceived barriers that could deter the use of CF are related to the implementation of the CF campaign, although contextual constraints have been mentioned.
Field-Induced Single-Ion Magnet Phenomenon in Hexabromo- and Hexaiodorhenate(IV) Complexes
Authors: Rojas-Dotti Carlos , Sanchis-Perucho Adrián , Orts-Arroyo Marta , Moliner Nicolás , González Ricardo , Lloret Francesc , Martínez-Lillo José
Published: April 2020

Two mononuclear ReIV complexes of general formula (PPh4)2[ReX6] [PPh4+ = tetraphenylphosphonium cation, X = Br (1) and I (2)] have been prepared and structurally and magnetically characterised. Both compounds crystallise in the triclinic system with space group Pī. Their structures are made up of hexahalorhenate(IV), [ReX6]2−, anions, and bulky PPh4+ cations. Each ReIV ion in 1 and 2 is six-coordinate and bonded to six halide ions in a quasi regular octahedral geometry. In their crystal packing, the [ReX6]2− anions are well separated from each other through the organic cations, generating alternated anionic and cationic layers, and no intermolecular Re−X···X−Re interactions are present. Variable-temperature dc magnetic susceptibility measurements performed on microcrystalline samples of 1 and 2 show a very similar magnetic behaviour, which is typical of noninteracting mononuclear ReIV complexes with S = 3/2. Ac magnetic susceptibility measurements reveal the slow relaxation of the magnetisation in the presence of external dc fields for 1 and 2, hence indicating the occurrence of the field-induced single-ion magnet (SIM) phenomenon in these hexabromo- and hexaiodorhenate(IV) complexes.
A Parametric Kind of Fubini Polynomials of a Complex Variable
Authors: Sharma Sunil Kumar , Khan Waseem A. , Ryoo Cheon Seoung
Published: April 2020

In this paper, we propose a parametric kind of Fubini polynomials by defining the two specific generating functions. We also investigate some analytical properties (for example, summation formulae, differential formulae and relationships with other well-known polynomials and numbers) for our introduced polynomials in a systematic way. Furthermore, we consider some relationships for parametric kind of Fubini polynomials associated with Bernoulli, Euler, and Genocchi polynomials and Stirling numbers of the second kind.
A Fixed-Point Approach to the Hyers–Ulam Stability of Caputo–Fabrizio Fractional Differential Equations
Authors: Liu Kui , Fečkan Michal , Wang JinRong
Published: April 2020

In this paper, we study Hyers–Ulam and Hyers–Ulam–Rassias stability of nonlinear Caputo–Fabrizio fractional differential equations on a noncompact interval. We extend the corresponding uniqueness and stability results on a compact interval. Two examples are given to illustrate our main results.
Calibration of Advanced Yield Criteria Using Uniaxial and Heterogeneous Tensile Test Data
Authors: Maček Andraž , Starman Bojan , Mole Nikolaj , Halilovič Miroslav
Published: April 2020

Conventionally, plastic anisotropy is calibrated by using standard uniaxial tensile and biaxial test results. Alternatively, heterogeneous strain field specimens in combination with full-field measurements can be used for this purpose. As reported by the literature, such an approach reduces the number of required tests enormously, but it is challenging to obtain reliable results. This paper presents an alternative methodology, which represents a compromise between the conventional and heterogeneous strain field calibration technique. The idea of the method is to use simple tests, which can be conducted on the uniaxial testing machine, and to avoid the use of advanced measuring equipment. The procedure is accomplished by conducting standard tensile tests, which are simple and reliable, and by a novel heterogeneous strain field tensile test, to calibrate the biaxial stress state. Moreover, only two of the parameters required for full characterisation need to be inversely identified from the test response; the other parameters are directly determined from the uniaxial tensile test results. This way, a dimension of optimization space is reduced substantially, which increases the robustness and effectiveness of the optimization algorithm.
Expression of Bacillus cereus Virulence-Related Genes in an Ocular Infection-Related Environment
Authors: Coburn Phillip S. , Miller Frederick C. , Enty Morgan A. , Land Craig , LaGrow Austin L. , Mursalin Md Huzzatul , Callegan Michelle C.
Published: April 2020

Bacillus cereus produces many factors linked to pathogenesis and is recognized for causing gastrointestinal toxemia and infections. B. cereus also causes a fulminant and often blinding intraocular infection called endophthalmitis. We reported that the PlcR/PapR system regulates intraocular virulence, but the specific factors that contribute to B. cereus virulence in the eye remain elusive. Here, we compared gene expression in ex vivo vitreous humor with expression in Luria Bertani (LB) and Brain Heart Infusion (BHI) broth by RNA-Seq. The expression of several cytolytic toxins in vitreous was less than or similar to levels observed in BHI or LB. Regulators of virulence genes, including PlcR/PapR, were expressed in vitreous. PlcR/PapR was expressed at low levels, though we reported that PlcR-deficient B. cereus was attenuated in the eye. Chemotaxis and motility genes were expressed at similar levels in LB and BHI, but at low to undetectable levels in vitreous, although motility is an important phenotype for B. cereus in the eye. Superoxide dismutase, a potential inhibitor of neutrophil activity in the eye during infection, was the most highly expressed gene in vitreous. Genes previously reported to be important to intraocular virulence were expressed at low levels in vitreous under these conditions, possibly because in vivo cues are required for higher level expression. Genes expressed in vitreous may contribute to the unique virulence of B. cereus endophthalmitis, and future analysis of the B. cereus virulome in the eye will identify those expressed in vivo, which could potentially be targeted to arrest virulence.
Isolation, Identification and Characterization of Endophytic Bacterium Rhizobium oryzihabitans sp. nov., from Rice Root with Biotechnological Potential in Agriculture
Authors: Zhao Juanjuan , Zhao Xia , Wang Junru , Gong Qi , Zhang Xiaoxia , Zhang Guishan
Published: April 2020

A flagellate, rod–shaped bacterium designated strain M15T was isolated from rice roots. Phylogenetic analysis based on the sequences of the 16S rRNA, housekeeping genes and genomes showed that the isolate belonged to the genus Rhizobium, with the highest 16S rRNA similarity to Rhizobium radiobacter LMG140T (99.64%) and Rhizobium pusense NRCPB10T (99.36%), respectively. The complete genome of the strain M15T has a 59.28% G+C content, and the highest average nucleotide identity (ANI) and DNA-DNA relatedness (DDH) values were obtained with R. radiobacter LMG140T (88.11%, 54.80%), R. pusense NRCPB10T (86.00%, 53.00%) and R. nepotum 39/7T (88.80%, 49.80%), respectively. Plant growth-promoting characteristics tests showed that the strain M15T produced siderophore, 1–aminocyclopropane–1–carboxylate (ACC) deaminase and indole-3-acetic acid (IAA) and also produced some secondary metabolites according to the analysis of the comparative genomes. Based on the data mentioned above, we proposed that the strain M15T represented a novel species of the genus Rhizobium, named Rhizobium oryzihabitans sp. nov. The type strain is M15T (=JCM 32903T  = ACCC 60121T), and the strain M15T can be a novel biofertilizer Rhizobium to reduce the use of synthetic fertilizers for plant growth promotion.
Taxogenomics of the Genus Cyclobacterium: Cyclobacterium xiamenense and Cyclobacterium halophilum as Synonyms and Description of Cyclobacterium plantarum sp. nov.
Authors: Shahinpei Azadeh , Amoozegar Mohammad Ali , Mirfeizi Leila , Nikou Mahdi Moshtaghi , Ventosa Antonio , Sánchez-Porro Cristina
Published: April 2020

The genus Cyclobacterium belongs to the phylum Bacteroidetes and includes eight species. Our study, based on the genomic parameters in silico DNA–DNA hybridization (GGDC), average nucleotide identity (OrthoANI), and average amino acid identity (AAI), confirmed that all current species of Cyclobacterium belong to this genus and constitute a coherent phylogenomic group, but with species forming two separate branches. In addition, the genome-based analyses revealed that Cyclobacterium xiamenense and Cyclobacterium halophilum are members of the same species. Besides, we carried out a taxonomic characterization of the new strain GBPx2T, isolated from the halophytic plant Salicornia sp. Analysis of its 16S rRNA gene sequence showed the highest sequence similarity (97.5%) to Cyclobacterium lianum HY9T. Percentages of GGDC and OrthoANI between strain GBPx2T and species of the genus Cyclobacterium were lower than the threshold value for species delineation. The DNA G+C content was 43.0 mol%. The polar lipids included phosphatidylethanolamine as well as one unidentified phospholipid and four unidentified lipids, and its major cellular fatty acids were iso-C15:0 and summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH). The only quinone present was menaquinone 7. Based on a combination of phenotypic, chemotaxonomic, and phylogenomic features, the GBPx2T strain represents a novel species of the genus Cyclobacterium, for which the name Cyclobacterium plantarum sp. nov. is proposed. The type strain of Cyclobacterium plantarum is GBPx2T (= IBRC-M 10634T = LMG 28551T).
Engineering Yarrowia lipolytica for the Synthesis of Glutathione from Organic By-Products
Authors: Do Diem T. H. , Fickers Patrick
Published: April 2020

Tripeptide glutathione, which plays important roles in many cellular mechanisms, is also a biotechnology-oriented molecule with applications in medicine, food and cosmetic. Here, the engineering of the yeast Yarrowia lipolytica for the production of this metabolite at high titer values from various agro-industrial by-products is reported. The constitutive overexpression of the glutathione biosynthetic genes GSH1 and GSH2 encoding respectively γ-glutamylcysteine synthetase and glutathione synthetase, together with the INU1 gene from Kluyveromyces marxianus encoding inulinase yielded a glutathione titer value and a productivity of 644 nmol/mg protein and 510 µmol/gDCW, respectively. These values were obtained during bioreactor batch cultures in a medium exclusively comprising an extract of Jerusalem artichoke tuber, used as a source of inulin, and ammonium sulfate, used as a nitrogen source.
Long-Term Incubation PrPCWD with Soils Affects Prion Recovery but Not Infectivity
Authors: Kuznetsova Alsu , McKenzie Debbie , Cullingham Catherine , Aiken Judd M.
Published: April 2020

Chronic wasting disease (CWD) is a contagious prion disease of cervids. The infectious agent is shed from animals at the preclinical and clinical stages of disease where it persists in the environment as a reservoir of CWD infectivity. In this study, we demonstrate that long-term incubation of CWD prions (generated from tg-mice infected with deer or elk prions) with illite, montmorillonite (Mte) and whole soils results in decreased recovery of PrPCWD, suggesting that binding becomes more avid and irreversible with time. This continual decline of immunoblot PrPCWD detection did not correlate with prion infectivity levels. Bioassay showed no significant differences in incubation periods between mice inoculated with 1% CWD brain homogenate (BH) and with the CWD-BH pre-incubated with quartz or Luvisolic Ae horizon for 1 or 30 weeks. After 55 weeks incubation with Chernozem and Luvisol, bound PrPCWD was not detectable by immunoblotting but remained infectious. This study shows that although recovery of PrPCWD bound to soil minerals and whole soils with time become more difficult, prion infectivity is not significantly altered. Detection of prions in soil is, therefore, not only affected by soil type but also by length of time of the prion–soil interaction.
Drug Conjugates for Targeting Eph Receptors in Glioblastoma
Authors: Sharma Puja , Roberts Callie , Herpai Denise , Fokt Izabela D. , Priebe Waldemar , Debinski Waldemar
Published: April 2020

Glioblastoma (GBM) is a complex and heterogeneous tumor that warrants a comprehensive therapeutic approach for treatment. Tumor-associated antigens offer an opportunity to selectively target various components of the GBM microenvironment while sparing the normal cells within the central nervous system. In this study, we conjugated a multivalent vector protein, QUAD 3.0, that can target four receptors: EphA3, EphA2, EphB2, and also IL-13RA2, spanning virtually 100% of the GBM microenvironment, to doxorubicin derivatives. The conjugates effectively bound to all four receptors, although to varying degrees, and delivered cytotoxic loads to both established and patient-derived GBM cell lines, with IC50 values in the low nM range. The conjugates were also non-toxic to animals. We anticipate that the QUAD 3.0 Dox conjugates will be further used in preclinical models and possibly clinics in the foreseeable future.
Research of Constructive and Technological Methods for Forming a Silicon Disk Resonator with Whispering Gallery Modes
Authors: Yakuhina Anastasia , Kadochkin Alexey , Amelichev Vladimir , Gorelov Dmitry , Generalov Sergey
Published: April 2020

This article presents the results of a computer simulation of whispering gallery modes in the structure of a silicon disk resonator with a wedge-shaped profile made on a silicon-on-isolator base (SOI). The rationale for the choice of silicon as a material for its manufacturing is given. The results of the study of the influence of the wedge angle on the whispering gallery mode parameters (WGM) are presented. The optimum wedge angle of a silicon disk resonator is determined, which ensures the minimum loss and maximum mode stability. The technological aspects of plasma-chemical etching processes for forming a wedge-shaped profile of the edge of a silicon disk resonator are studied.
Comparative Analysis of the Complete Plastid Genome of Five Bupleurum Species and New Insights into DNA Barcoding and Phylogenetic Relationship
Authors: Li Jun , Xie Deng-Feng , Guo Xian-Lin , Zheng Zhen-Ying , He Xing-Jin , Zhou Song-Dong
Published: April 2020

Bupleurum L. (Apiaceae) is a perennial and herbal genus, most species of which have high medicinal value. However, few studies have been performed using plastome data in this genus, and the phylogenetic relationships have always been controversial. In this study, the plastid genomes of Bupleurum chinense and Bupleurum commelynoideum were sequenced, and their gene content, order, and structure were counted and analyzed. The only three published Bupleurum species (B. boissieuanum, B. falcatum, and B. latissimum) and other fifteen allied species were selected to conduct a series of comparative and phylogenetic analyses. The genomes of B. chinense and B. commelynoideum were 155,869 and 155,629 bp in length, respectively, both of which had a typical quadripartite structure. The genome length, structure, guanine and cytosine (GC) content, and gene distribution were highly similar to the other three Bupleurum species. The five Bupleurum species had nearly the same codon usages, and eight regions (petN-psbM, rbcL-accD, ccsA-ndhD, trnK(UUU)-rps16, rpl32-trnL(UAG)-ccsA, petA-psbJ, ndhF-rpl32, and trnP(UGG)-psaJ-rpl33) were found to possess relatively higher nucleotide diversity, which may be the promising DNA barcodes in Bupleurum. Phylogenetic analysis revealed that all Bupleurum species clustered into a monophyletic clade with high bootstrap support and diverged after the Chamaesium clade. Overall, our study provides new insights into DNA barcoding and phylogenetic relationship between Bupleurum and its related genera, and will facilitate the population genomics, conservation genetics, and phylogenetics of Bupleurum in Apiaceae.
Mosquito Larvicidal Activity, Antimicrobial Activity, and Chemical Compositions of Essential Oils from Four Species of Myrtaceae from Central Vietnam
Authors: An Nguyen Thi Giang , Huong Le Thi , Satyal Prabodh , Tai Thieu Anh , Dai Do Ngoc , Hung Nguyen Huy , Ngoc Nguyen Thi Bich , Setzer William N.
Published: April 2020

Mosquitoes are important vectors of several diseases, and control of these insects is imperative for human health. Insecticides have proven useful in controlling mosquito populations, but insecticide resistance and environmental concerns are increasing. Additionally, emerging and re-emerging microbial infections are problematic. Essential oils have been shown to be promising mosquito larvicidal agents as well as antimicrobial agents. In this work, the essential oils from four species of Myrtaceae (Baeckea frutescens, Callistemon citrinus, Melaleuca leucadendra, and Syzygium nervosum) growing wild in central Vietnam have been obtained by hydrodistillation and analyzed by gas chromatographic techniques. The essential oils have been screened for mosquito larvicidal activity against Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus, and for antimicrobial activity against Enterococcus faecalis, Staphylococcus aureus, and Candida albicans. Callistemon citrinus fruit essential oil, rich in α-pinene (35.1%), 1,8-cineole (32.4%), limonene (8.2%), and α-terpineol (5.8%) showed good larvicidal activity with 24-h LC50 = 17.3 μg/mL against both Ae. aegypti and Cx. quinquefasciatus, and good antibacterial activity against E. faecalis (minimum inhibitory concentration (MIC) = 16 μg/mL) The 48-h larvicidal activities of M. leucadendra leaf essential oil, rich in α-eudesmol (17.6%), guaiol (10.9%), linalool (5.1%), (E)-caryophyllene (7.0%), and bulnesol (3.6%) were particularly notable, with LC50 of 1.4 and 1.8 μg/mL on Ae. aegypti and Cx. quinquefasciatus. Similarly, M. leucadendra bark essential oil, with α-eudesmol (24.1%) and guaiol (11.3%), showed good antibacterial activity against. E. faecalis. Both B. frutescens and C. citrinus leaf essential oils demonstrated anti-Candida activities with MIC values of 16 μg/mL. The results of this investigation suggest that essential oils derived from the Myrtaceae may serve as “green” alternatives for the control of mosquitoes and/or complementary antimicrobial agents.
Holographic Imaging of Insect Cell Cultures: Online Non-Invasive Monitoring of Adeno-Associated Virus Production and Cell Concentration
Authors: Pais Daniel A. M. , Galrão Paulo R. S. , Kryzhanska Anastasiya , Barbau Jérémie , Isidro Inês A. , Alves Paula M.
Published: April 2020

The insect cell-baculovirus vector system has become one of the favorite platforms for the expression of viral vectors for vaccination and gene therapy purposes. As it is a lytic system, it is essential to balance maximum recombinant product expression with harvest time, minimizing product exposure to detrimental proteases. With this purpose, new bioprocess monitoring solutions are needed to accurately estimate culture progression. Herein, we used online digital holographic microscopy (DHM) to monitor bioreactor cultures of Sf9 insect cells. Batches of baculovirus-infected Sf9 cells producing recombinant adeno-associated virus (AAV) and non-infected cells were used to evaluate DHM prediction capabilities for viable cell concentration, culture viability and AAV titer. Over 30 cell-related optical attributes were quantified using DHM, followed by a forward stepwise regression to select the most significant (p < 0.05) parameters for each variable. We then applied multiple linear regression to obtain models which were able to predict culture variables with root mean squared errors (RMSE) of 7 × 105 cells/mL, 3% for cell viability and 2 × 103 AAV/cell for 3-fold cross-validation. Overall, this work shows that DHM can be implemented for online monitoring of Sf9 concentration and viability, also permitting to monitor product titer, namely AAV, or culture progression in lytic systems, making it a valuable tool to support the time of harvest decision and for the establishment of controlled feeding strategies.
The Integration of Collaborative Robot Systems and Their Environmental Impacts
Authors: Grigore Lucian Stefanita , Priescu Iustin , Joita Daniela , Oncioiu Ionica
Published: April 2020

Today, industrial robots are used in dangerous environments in all sectors, including the sustainable energy sector. Sensors and processors collect and transmit information and data from users as a result of the application of robot control systems and sensory feedback. This paper proposes that the estimation of a collaborative robot system’s performance can be achieved by evaluating the mobility of robots. Scenarios have been determined in which an autonomous system has been used for intervention in crisis situations due to fire. The experimental model consists of three autonomous vehicles, two of which are ground vehicles and the other is an aerial vehicle. The conclusion of the research described in this paper highlights the fact that the integration of robotic systems made up of autonomous vehicles working in unstructured environments is difficult and at present there is no unitary analytical model.
Proteomics of Brucella
Authors: Poetsch Ansgar , Marchesini María Inés
Published: April 2020

Brucella spp. are Gram negative intracellular bacteria responsible for brucellosis, a worldwide distributed zoonosis. A prominent aspect of the Brucella life cycle is its ability to invade, survive and multiply within host cells. Comprehensive approaches, such as proteomics, have aided in unravelling the molecular mechanisms underlying Brucella pathogenesis. Technological and methodological advancements such as increased instrument performance and multiplexed quantification have broadened the range of proteome studies, enabling new and improved analyses, providing deeper and more accurate proteome coverage. Indeed, proteomics has demonstrated its contribution to key research questions in Brucella biology, i.e., immunodominant proteins, host-cell interaction, stress response, antibiotic targets and resistance, protein secretion. Here, we review the proteomics of Brucella with a focus on more recent works and novel findings, ranging from reconfiguration of the intracellular bacterial proteome and studies on proteomic profiles of Brucella infected tissues, to the identification of Brucella extracellular proteins with putative roles in cell signaling and pathogenesis. In conclusion, proteomics has yielded copious new candidates and hypotheses that require future verification. It is expected that proteomics will continue to be an invaluable tool for Brucella and applications will further extend to the currently ill-explored aspects including, among others, protein processing and post-translational modification.
From ‘Soft’ to ‘Hard’ to ‘Moderate’: Islam in the Dilemmas of Post-2011 Saudi Foreign Policy
Authors: Athanasoulia Stella
Published: April 2020

Due to its specific history, Saudi Arabia’s domestic and international politics are inextricably linked together. In the shaping and implementation of the kingdom’s essentially defensive international behavior, Islam has kept a central position as a legitimizing factor and as a tool of ‘soft power’ foreign policy. This paper firstly aims to explore the position that religion holds in the Saudi perceptions of stability in the post-2011 era, where safeguarding the status quo remained the priority, while the new administration responded to external pressure by shifting towards a ‘hard power’ foreign policy with questionable results. Furthermore, the paper explores the links between foreign policy choices and the promotion of a new, modern image for the kingdom whereas the regime has appropriated for itself the notion of ‘moderate Islam’ in order to restore its international image and attract investments for the ‘Vision 2030’ reform program. Finally, it proposes a discussion of the position of Islam in the future direction of the country, amidst an increasing authoritarian rule.
Chilaiditi’s Syndrome—What Every Endoscopist Should Know
Authors: George Jayan , Genever Ashleigh V , White Timothy J
Published: April 2020

Chilaiditi’s syndrome is a rare and often asymptomatic anomaly, typically found as an incidental radiographic sign (gas under the diaphragm) due to hepato-diaphragmatic interposition of the transverse colon. We report a case of Chilaiditi’s syndrome following colonoscopy presenting with severe abdominal pain, dyspnoea and radiograph findings similar to the presence of bowel perforation (appearance of gas under the hemidiaphragm on erect chest radiograph). Computed tomography (CT) evidence of Chilaiditi’s sign prevented unnecessary laparotomy.
Towards Understanding Benefit Sharing between Extractive Industries and Indigenous/Local Communities in the Arctic
Authors: Tysiachniouk Maria S. , Petrov Andrey N. , Gassiy Violetta
Published: April 2020

The aim of this Special Issue is to provide a comprehensive view of the benefit sharing and compensation mechanisms for the Indigenous Peoples and local communities in the Arctic and sub-Arctic regions due to industrial resource extraction. The papers cover the following topics: (1) Benefit-sharing frameworks in the Arctic. (2) Corporate social responsibility standards and benefit sharing by extractive industries in the Arctic. (3) Benefit sharing and international and national legislation. (4) The practice of implementing legislation to support Indigenous and local interests. (5) The methodologies for assessing compensation to Indigenous communities from extractive industries.
Grazing Management, Forage Production and Soil Carbon Dynamics
Authors: Ritchie Mark E.
Published: April 2020

Soil carbon pools remain a target for sequestering greenhouse gases, but appropriate land management options to achieve such sequestration remain uncertain. Livestock grazing can have profound positive or negative effects on soil carbon. Different models for assessing the influences of grazing are available, but few explicitly account for different management options on soil organic carbon (SOC). Here, I link a previous simple SOC dynamic model (SNAP) to a recent model of episodic grazing and its effects on primary production. The resulting combined model, called SNAPGRAZE, assesses the potential effects of grazing management on SOC across a range of climates with only eight climate, soil, and management input variables. SNAPGRAZE predicts that, at high stocking densities relative to those sustainable under continuous grazing and at higher mean annual temperature and precipitation, short-duration, high stocking density (SDHSD) grazing schemes can enhance forage production and increase stocks of soil organic carbon. Model predictions for current SOC, given a known 50 year grazing history, agrees well with data from nine private ranches in the North American Great Plains. SNAPGRAZE may provide a framework for exploring the consequences of grazing management for forage production and soil carbon dynamics.
Antarctic Supraglacial Lake Identification Using Landsat-8 Image Classification
Authors: Halberstadt Anna Ruth W. , Gleason Colin J. , Moussavi Mahsa S. , Pope Allen , Trusel Luke D. , DeConto Robert M.
Published: April 2020

Surface meltwater generated on ice shelves fringing the Antarctic Ice Sheet can drive ice-shelf collapse, leading to ice sheet mass loss and contributing to global sea level rise. A quantitative assessment of supraglacial lake evolution is required to understand the influence of Antarctic surface meltwater on ice-sheet and ice-shelf stability. Cloud computing platforms have made the required remote sensing analysis computationally trivial, yet a careful evaluation of image processing techniques for pan-Antarctic lake mapping has yet to be performed. This work paves the way for automating lake identification at a continental scale throughout the satellite observational record via a thorough methodological analysis. We deploy a suite of different trained supervised classifiers to map and quantify supraglacial lake areas from multispectral Landsat-8 scenes, using training data generated via manual interpretation of the results from k-means clustering. Best results are obtained using training datasets that comprise spectrally diverse unsupervised clusters from multiple regions and that include rock and cloud shadow classes. We successfully apply our trained supervised classifiers across two ice shelves with different supraglacial lake characteristics above a threshold sun elevation of 20°, achieving classification accuracies of over 90% when compared to manually generated validation datasets. The application of our trained classifiers produces a seasonal pattern of lake evolution. Cloud shadowed areas hinder large-scale application of our classifiers, as in previous work. Our results show that caution is required before deploying ‘off the shelf’ algorithms for lake mapping in Antarctica, and suggest that careful scrutiny of training data and desired output classes is essential for accurate results. Our supervised classification technique provides an alternative and independent method of lake identification to inform the development of a continent-wide supraglacial lake mapping product.
Comparison of Machine Learning Methods for Estimating Mangrove Above-Ground Biomass Using Multiple Source Remote Sensing Data in the Red River Delta Biosphere Reserve, Vietnam
Authors: Pham Tien Dat , Yokoya Naoto , Xia Junshi , Ha Nam Thang , Le Nga Nhu , Nguyen Thi Thu Trang , Dao Thi Huong , Vu Thuy Thi Phuong , Pham Tien Duc , Takeuchi Wataru
Published: April 2020

This study proposes a hybrid intelligence approach based on an extreme gradient boosting regression and genetic algorithm, namely, the XGBR-GA model, incorporating Sentinel-2, Sentinel-1, and ALOS-2 PALSAR-2 data to estimate the mangrove above-ground biomass (AGB), including small and shrub mangrove patches in the Red River Delta biosphere reserve across the northern coast of Vietnam. We used the novel extreme gradient boosting decision tree (XGBR) technique together with genetic algorithm (GA) optimization for feature selection to construct and verify a mangrove AGB model using data from a field survey of 105 sampling plots conducted in November and December of 2018 and incorporated the dual polarimetric (HH and HV) data of the ALOS-2 PALSAR-2 L-band and the Sentinel-2 multispectral data combined with Sentinel-1 (C-band VV and VH) data. We employed the root-mean-square error (RMSE) and coefficient of determination (R2) to evaluate the performance of the proposed model. The capability of the XGBR-GA model was assessed via a comparison with other machine-learning (ML) techniques, i.e., the CatBoost regression (CBR), gradient boosted regression tree (GBRT), support vector regression (SVR), and random forest regression (RFR) models. The XGBR-GA model yielded a promising result (R2 = 0.683, RMSE = 25.08 Mg·ha−1) and outperformed the four other ML models. The XGBR-GA model retrieved a mangrove AGB ranging from 17 Mg·ha−1 to 142 Mg·ha−1 (with an average of 72.47 Mg·ha−1). Therefore, multisource optical and synthetic aperture radar (SAR) combined with the XGBR-GA model can be used to estimate the mangrove AGB in North Vietnam. The effectiveness of the proposed method needs to be further tested and compared to other mangrove ecosystems in the tropics.
Ash Presence and Abundance Derived from Composite Landsat and Sentinel-2 Time Series and Lidar Surface Models in Minnesota, USA
Authors: Host Trevor K. , Russell Matthew B. , Windmuller-Campione Marcella A. , Slesak Robert A. , Knight Joseph F.
Published: April 2020

Ash trees (Fraxinus spp.) are a prominent species in Minnesota forests, with an estimated 1.1 billion trees in the state, totaling approximately 8% of all trees. Ash trees are threatened by the invasive emerald ash borer (Agrilus planipennis Fairmaire), which typically results in close to 100% tree mortality within one to five years of infestation. A detailed, wall-to-wall map of ash presence is highly desirable for forest management and monitoring applications. We used Google Earth Engine to compile Landsat time series analysis, which provided unique information on phenologic patterns across the landscape to identify ash species. Topographic position information derived from lidar was added to improve spatial maps of ash abundance. These input data were combined to produce a classification map and identify the abundance of ash forests that exist in the state of Minnesota. Overall, 12,524 km2 of forestland was predicted to have greater than 10% probability of ash species present. The overall accuracy of the composite ash presence/absence map was 64% for all ash species and 72% for black ash, and classification accuracy increased with the length of the time series. Average height derived from lidar was the best model predictor for ash basal area (R2 = 0.40), which, on average, was estimated as 16.1 m2 ha−1. Information produced from this map will be useful for natural resource managers and planners in developing forest management strategies which account for the spatial distribution of ash on the landscape. The approach used in this analysis is easily transferable and broadly scalable to other regions threatened with forest health problems such as invasive insects.
Detection of Moving Load on Pavement Using Piezoelectric Sensors
Authors: Xiang Tao , Huang Kangxu , Zhang He , Zhang Yangyang , Zhang Yinnan , Zhou Yuhui
Published: April 2020

More and more researches have been carried out recently on Weigh-In-Motion (WIM) technology for solving the traffic safety problems caused by overload. In this article, we aim to study the measurement accuracy of the WIM system. Based on the electromechanical theory and elastic half-space method, we establish a theoretical model of multi-layer structure to investigate the correlation between the output voltage of the piezoelectric sensor and the applied force. In addition, we performed cyclic and moving load experiments to verify the accuracy of the analytical calculations. The load magnitude identified by this theoretical model matched the experiments very well, which shows that this model is effective for the WIM system. In addition, we proved that the load frequency is an important factor affecting the measurement accuracy of the sensor, which further enables us to design more suitable sensors for certain use scenarios.
Development of an Aptamer Based Luminescent Optical Fiber Sensor for the Continuous Monitoring of Hg2+ in Aqueous Media
Authors: De Acha Nerea , Elosúa César , Arregui Francisco J.
Published: April 2020

A fluorescent optical fiber sensor for the detection of mercury (Hg2+) ions in aqueous solutions is presented in this work. The sensor was based on a fluorophore-labeled thymine (T)-rich oligodeoxyribonucleotide (ON) sequence that was directly immobilized onto the tip of a tapered optical fiber. In the presence of mercury ions, the formation of T–Hg2+-T mismatches quenches the fluorescence emission by the labeled fluorophore, which enables the measurement of Hg2+ ions in aqueous solutions. Thus, in contrast to commonly designed sensors, neither a fluorescence quencher nor a complementary ON sequence is required. The sensor presented a response time of 24.8 seconds toward 5 × 10−12 M Hg2+. It also showed both good reversibility (higher than the 95.8%) and selectivity: the I0/I variation was 10 times higher for Hg2+ ions than for Mn2+ ions. Other contaminants examined (Co2+, Ag+, Cd2+, Ni2+, Ca2+, Pb2+, Mn2+, Zn2+, Fe3+, and Cu2+) presented an even lower interference. The limit of detection of the sensor was 4.73 × 10−13 M Hg2+ in buffer solution and 9.03 × 10−13 M Hg2+ in ultrapure water, and was also able to detect 5 × 10−12 M Hg2+ in tap water.
Why Are Viscosity and Nonlinearity Bound to Make an Impact in Clinical Elastographic Diagnosis?
Authors: Rus Guillermo , Faris Inas H. , Torres Jorge , Callejas Antonio , Melchor Juan
Published: April 2020

The adoption of multiscale approaches by the biomechanical community has caused a major improvement in quality in the mechanical characterization of soft tissues. The recent developments in elastography techniques are enabling in vivo and non-invasive quantification of tissues’ mechanical properties. Elastic changes in a tissue are associated with a broad spectrum of pathologies, which stems from the tissue microstructure, histology and biochemistry. This knowledge is combined with research evidence to provide a powerful diagnostic range of highly prevalent pathologies, from birth and labor disorders (prematurity, induction failures, etc.), to solid tumors (e.g., prostate, cervix, breast, melanoma) and liver fibrosis, just to name a few. This review aims to elucidate the potential of viscous and nonlinear elastic parameters as conceivable diagnostic mechanical biomarkers. First, by providing an insight into the classic role of soft tissue microstructure in linear elasticity; secondly, by understanding how viscosity and nonlinearity could enhance the current diagnosis in elastography; and finally, by compounding preliminary investigations of those elastography parameters within different technologies. In conclusion, evidence of the diagnostic capability of elastic parameters beyond linear stiffness is gaining momentum as a result of the technological and imaging developments in the field of biomechanics.