bluuebunny/crossref_metadata_2025 huggingface dataset:
Testing the Complete Plastome for Species Discrimination, Cryptic Species Discovery and Phylogenetic Resolution in Cephalotaxus (Cephalotaxaceae)
Authors: Wang Jie , Fu Chao-Nan , Mo Zhi-Qiong , Möller Michael , Yang Jun-Bo , Zhang Zhi-Rong , Li De-Zhu , Gao Lian-Ming
Published: May 2022

Species of Cephalotaxus have great economic and ecological values. However, the taxonomy and interspecific phylogenetic relationships within the genus have been controversial and remained not fully resolved until now. To date, no study examined the efficiency of the complete plastome as super-barcode across Cephalotaxus species with multiple samples per taxon. In this study, we have evaluated the complete plastome in species discrimination and phylogenetic resolution in Cephalotaxus by including 32 individuals of all eight recognized species and five varieties following Farjon’s classification (2010) with multiple samples per taxon. Our results indicated that not all species recognized in recent taxonomic revisions of Cephalotaxus could be distinguished and not all were monophyletic. Based on the plastome phylogeny, a new taxonomic classification for the genus comprising nine species and two varieties, including a cryptic species, was proposed. The phylogeny also resolved all interspecific relationships. Compared to the plastome based classification, standard DNA barcodes, alone or in combination, only recognized a maximum of seven out of the nine species. Moreover, two highly variable single loci, ycf1 and rps16, each alone achieved full species discrimination. With the moderate length of 1079 bp, rps16 is proposed as a specific barcode to discriminate Cephalotaxus species. The super-barcodes and specific barcode candidates will aid in the identification of endangered Cephalotaxus species, and to help focus conservation measures.
Glycine max Homologs of DOESN'T MAKE INFECTIONS 1, 2, and 3 Function to Impair Heterodera glycines Parasitism While Also Regulating Mitogen Activated Protein Kinase Expression
Authors: Khatri Rishi , Pant Shankar R. , Sharma Keshav , Niraula Prakash M. , Lawaju Bisho R. , Lawrence Kathy S. , Alkharouf Nadim W. , Klink Vincent P.
Published: May 2022

Glycine maxroot cells developing into syncytia through the parasitic activities of the pathogenic nematodeHeterodera glycinesunderwent isolation by laser microdissection (LM). Microarray analyses have identified the expression of aG. max DOESN'T MAKE INFECTIONS3(DMI3) homolog in syncytia undergoing parasitism but during a defense response.DMI3encodes part of the common symbiosis pathway (CSP) involvingDMI1, DMI2, and other CSP genes. The identifiedDMIgene expression, and symbiosis role, suggests the possible existence of commonalities between symbiosis and defense.G. maxhas 3DMI1, 12DMI2, and 2DMI3paralogs. LM-assisted gene expression experiments of isolated syncytia under further examination here showG. max DMI1-3, DMI2-7, andDMI3-2expression occurring during the defense response in theH. glycines-resistant genotypesG.max[Peking/PI548402]andG.max[PI88788]indicating a broad and consistent level of expression of the genes. Transgenic overexpression (OE) ofG. max DMI1-3, DMI2-7, andDMI3-2impairsH. glycinesparasitism. RNA interference (RNAi) ofG. max DMI1-3, DMI2-7, andDMI3-2increasesH. glycinesparasitism. The combined opposite outcomes reveal a defense function for these genes. Prior functional transgenic analyses of the 32-memberG. max mitogen activated protein kinase(MAPK) gene family has determined that 9 of them act in the defense response toH. glycinesparasitism, referred to as defenseMAPKs. RNA-seq analyses of root RNA isolated from the 9G. maxdefenseMAPKs undergoing OE or RNAi reveal they alter the relative transcript abundances (RTAs) of specificDMI1, DMI2, andDMI3paralogs. In contrast, transgenically-manipulatedDMI1-3, DMI2-7, andDMI3-2expression influencesMAPK3-1andMAPK3-2RTAs under certain circumstances. The results showG. maxhomologs of the CSP, and defense pathway are linked, apparently involving co-regulated gene expression.
An Improved Agrobacterium-Mediated Transformation and Genome-Editing Method for Maize Inbred B104 Using a Ternary Vector System and Immature Embryos
Authors: Kang Minjeong , Lee Keunsub , Finley Todd , Chappell Hal , Veena Veena , Wang Kan
Published: May 2022

For maize genome-editing and bioengineering, genetic transformation of inbred genotypes is most desired due to the uniformity of genetic background in their progenies. However, most maize inbred lines are recalcitrant to tissue culture and transformation. A public, transformable maize inbred B104 has been widely used for genome editing in recent years. This is primarily due to its high degree of genetic similarity shared with B73, an inbred of the reference genome and parent of many breeding populations. Conventional B104 maize transformation protocol requires 16–22 weeks to produce rooted transgenic plants with an average of 4% transformation frequency (number of T0 plants per 100 infected embryos). In this Method paper, we describe an advanced B104 transformation protocol that requires only 7–10 weeks to generate transgenic plants with an average of 6.4% transformation frequency. Over 66% of transgenic plants carried CRISPR/Cas9-induced indel mutations on the target gene, demonstrating that this protocol can be used for genome editing applications. Following the detailed and stepwise procedure described here, this quick and simplified method using the Agrobacterium ternary vector system consisting of a T-DNA binary vector and a compatible helper plasmid can be readily transferable to interested researchers.
Shifting Rice Cropping Systems Mitigates Ecological Footprints and Enhances Grain Yield in Central China
Authors: Zhou Yong , Liu Ke , Harrison Matthew Tom , Fahad Shah , Gong Songling , Zhu Bo , Liu Zhangyong
Published: May 2022

Intensive cereal production has brought about increasingly serious environmental threats, including global warming, environmental acidification, and water shortage. As an important grain producer in the world, the rice cultivation system in central China has undergone excessive changes in the past few decades. However, few articles focused on the environmental impacts of these shifts from the perspective of ecological footprints. In this study, a 2-year field trial was carried out in Hubei province, China, to gain insight into carbon footprint (CF), nitrogen footprint (NF), and water footprint (WF) performance. The three treatments were, namely, double-rice system (DR), ratoon rice system (RR), and rice-wheat system (RW). Results demonstrated that RR significantly increased the grain yield by 10.22–15.09% compared with DR, while there was no significant difference in the grain yield between RW and DR in 2018–2019. All of the calculation results by three footprint approaches followed the order: RR < RW < DR; meanwhile, RR was always significantly lower than DR. Methane and NH3 field emissions were the hotspots of CF and NF, respectively. Blue WF accounts for 40.90–42.71% of DR, which was significantly higher than that of RR and RW, primarily because DR needs a lot of irrigation water in both seasons. The gray WF of RW was higher than those of DR and RR, mainly due to the higher application rate of N fertilizer. In conclusion, RR possesses the characteristics of low agricultural inputs and high grain yield and can reduce CF, NF, and WF, considering the future conditions of rural societal developments and rapid demographic changes; we highlighted that the RR could be a cleaner and sustainable approach to grain production.
Establishing Tetraploid Embryogenic Cell Lines of Magnolia officinalis to Facilitate Tetraploid Plantlet Production and Phenotyping
Authors: Gao Yanfen , Ma Junchao , Chen Jiaqi , Xu Qian , Jia Yanxia , Chen Hongying , Li Weiqi , Lin Liang
Published: May 2022

The production of synthetic polyploids for plant breeding is compromised by high levels of mixoploids and low numbers of solid polyploid regenerants during in vitro induction. Somatic embryogenesis could potentially contribute to the maximization of solid polyploid production due to the single cell origin of regenerants. In the present study, a novel procedure for establishing homogeneous tetraploid embryogenic cell lines in Magnolia officinalis has been established. Embryogenic cell aggregate (ECA) about 100–200 μm across, and consisting of dozens of cells, regenerated into a single colony of new ECAs and somatic embryos following colchicine treatment. Histological analysis indicated that the few cells that survived some colchicine regimes still regenerated to form a colony. In some colonies, 100% tetraploid somatic embryos were obtained without mixoploid formation. New granular ECA from single colonies with 100% tetraploid somatic embryos were isolated and cultured individually to proliferate into cell lines. These cell lines were confirmed to be homogeneous tetraploid by flow cytometry. Many tetraploid somatic embryos and plantlets were differentiated from these cell lines and the stability of ploidy level through the somatic embryogenesis process was confirmed by flow cytometry and chromosome counting. The establishment of homogeneous polyploid cell lines, which were presumed to represent individual polyploidization events, might expand the phenotypic variations of the same duplicated genome and create novel breeding opportunities using newly generated polyploid plantlets.
Reliability and Validation of the Child Eating Behavior Questionnaire in 3- to 6-Year-Old Spanish Children
Authors: Jimeno-Martínez Andrea , Maneschy Ivie , Moreno Luis A. , Bueno-Lozano Gloria , De Miguel-Etayo Pilar , Flores-Rojas Katherine , Jurado-Castro Jose Manuel , de Lamas Carmela , Vázquez-Cobela Rocio , Martinez-Lacruz Raúl , Portoles Olga , Martínez J. Alfredo , Navas-Carretero Santiago , Schröder Helmut , Fitó Montserrat , Babio Nancy , Salas-Salvadó Jordi , Leis Rosaura , Gil-Campos Mercedes , Rupérez Azahara I.
Published: May 2022

Introduction

Eating behavior is often established during the first years of life. Therefore, it is important to make a research on it to understand the relationships that children have with food and how this can contribute to prevent the development of childhood obesity. An appropriate assessment of eating behavior can be achieved using the “Child Eating Behavior Questionnaire” (CEBQ). This questionnaire has been validated in several populations and languages, but it has never been translated, adapted, and validated for Spanish children.

Aim

To evaluate the reliability and internal consistency of the CEBQ questionnaire, culturally adapted and translated into Spanish (Spain), in Spanish families with children aged 3 to 6 years, as well as its association with children’s body mass index (BMI) to test its construct validity.

Materials and Methods

Children between 3 and 6 years old were recruited from the ongoing MELI-POP randomized controlled clinical trial, as well as from public schools located in middle class neighborhoods of Zaragoza, Spain, to complete the sample. Sociodemographic characteristics and anthropometric measures were obtained according to standardized methods. The 35-item CEBQ questionnaire was completed twice with a time difference of 3 weeks between each response. Statistical analyses included the evaluation of internal consistency and reliability of the questionnaire, a confirmatory factor analysis, and the association between the different CEBQ scales and the children’s BMI.

Results

A total of 197 children completed variables; 97 of them were boys (49.2%) and 100 girls (50.8%). Mean age of the total sample was 4.7 ± 0.9 years. There was a high test-re-test reliability of the questionnaire with values close to 1, with an average of 0.66 and a good internal consistency (Cronbach alpha with values above 0.7), so that a high reliability is established between the items in each scale. A gradual positive association was found between the score of different “pro-intake” scales of the CEBQ: “Food Responsiveness,” “Emotional Overeating,” and “Enjoyment of food” and the children’s BMI; at the opposite, negative associations were observed between BMI and the score of anti-intake scales “Satiety Responsiveness,” “Slowness in Eating,” and “Emotional Undereating.”

Conclusion

The Spanish version of the CEBQ is a useful tool to assess the eating behavior of Spanish children because the high reliability and internal validity. There is a significant association between eating behavior and BMI in Spanish children.
The Examination of Conscience: A Preliminary Study on the Effects on Metamemory After a 2-Week Practice
Authors: De Pisapia Nicola , Dall’Avanzi Martina
Published: May 2022

Examination of conscience (EC) is a contemplative practice that consists in examining daily the actions performed during the day (evening examination, immediately before going to bed) and the actions to be performed on the coming day (morning examination, immediately after sleep). While research on contemplative practices such as mindfulness has seen a large increase of studies in recent years, research into the psychological effects related to the practice of the EC has been largely ignored in the scientific literature. On the other hand, on a careful historical and philosophical analysis, it appears evident that references to it abound in many cultural contexts and in different eras. Here, we examined the effects of a 2-week program of this practice that participants performed using a digital application developed ad hoc for this experiment. A control group performed an activity of the same duration, also with the support of a digital application, but which consisted of listening to audio excerpts of an Italian literary novel. Measurements taken from both groups before and after the 2-week period consisted of self-assessments of metamemory capacity, that is, awareness and knowledge of their own memory processes. Results showed that participants reported a significant decrease in two properties of their metamemory after training, specifically vividness and coherence. No other significant changes were found between the two groups. Specifically, we found no differences in other metamemory characteristics, no differences in prospective or retrospective memory, and no differences in changes in stress levels. These results, although preliminary, suggest that a relatively short period of EC does indeed make people metacognitively more aware of the limitations and errors of their memory, in particular concerning vividness and coherence.
Language Entropy Relates to Behavioral and Pupil Indices of Executive Control in Young Adult Bilinguals
Authors: van den Berg Floor , Brouwer Jelle , Tienkamp Thomas B. , Verhagen Josje , Keijzer Merel
Published: May 2022

Introduction

It has been proposed that bilinguals’ language use patterns are differentially associated with executive control. To further examine this, the present study relates the social diversity of bilingual language use to performance on a color-shape switching task (CSST) in a group of bilingual university students with diverse linguistic backgrounds. Crucially, this study used language entropy as a measure of bilinguals’ language use patterns. This continuous measure reflects a spectrum of language use in a variety of social contexts, ranging from compartmentalized use to fully integrated use.

Methods

Language entropy for university and non-university contexts was calculated from questionnaire data on language use. Reaction times (RTs) were measured to calculate global RT and switching and mixing costs on the CSST, representing conflict monitoring, mental set shifting, and goal maintenance, respectively. In addition, this study innovatively recorded a potentially more sensitive measure of set shifting abilities, namely, pupil size during task performance.

Results

Higher university entropy was related to slower global RT. Neither university entropy nor non-university entropy were associated with switching costs as manifested in RTs. However, bilinguals with more compartmentalized language use in non-university contexts showed a larger difference in pupil dilation for switch trials in comparison with non-switch trials. Mixing costs in RTs were reduced for bilinguals with higher diversity of language use in non-university contexts. No such effects were found for university entropy.

Discussion

These results point to the social diversity of bilinguals’ language use as being associated with executive control, but the direction of the effects may depend on social context (university vs. non-university). Importantly, the results also suggest that some of these effects may only be detected by using more sensitive measures, such as pupil dilation. The paper discusses theoretical and practical implications regarding the language entropy measure and the cognitive effects of bilingual experiences more generally, as well as how methodological choices can advance our understanding of these effects.
Psychological Contract, Self-Efficacy, Job Stress, and Turnover Intention: A View of Job Demand-Control-Support Model
Authors: Shao Lijin , Guo Hui , Yue Xiaoyao , Zhang Zhaohua
Published: May 2022

The outbreak of Coronavirus disease 2019 (COVID-19) has caused enterprises to face more challenges, such as operational management, production and sales management, and human resource management, among other issues. In the context of the global knowledge economy, employees with high knowledge and skills have become an important source of corporate growth and breakthroughs. However, employees may intend to transfer to other companies due to the pressure of the external and internal environments, so the main topic explored by this paper will be the change of employees' turnover intention. The purpose of this study was to explore the influence mechanism that propels the employees' self-efficacy, job stress, and turnover intention, and the moderating effect of transformational leadership. A total of 553 valid responses from several information service companies in China are collected via purposive sampling and used in the data analysis. This study conducts partial least squares structural equation modeling partial least squares structural equation modeling (PLS-SEM) to analyze collected data. The results of the path analysis with structural equation modeling show that employees' psychological contracts have a positive impact on the self-efficacy and a negative impact on the job stress. Employees' self-efficacy has a negative impact on job stress and turnover intention; transformational leadership plays a significant moderator in the research framework. Based on research findings, the theoretical and managerial implications are presented.
Improving the Accuracy of Ensemble Machine Learning Classification Models Using a Novel Bit-Fusion Algorithm for Healthcare AI Systems
Authors: Mishra Sashikala , Shaw Kailash , Mishra Debahuti , Patil Shruti , Kotecha Ketan , Kumar Satish , Bajaj Simi
Published: May 2022

Healthcare AI systems exclusively employ classification models for disease detection. However, with the recent research advances into this arena, it has been observed that single classification models have achieved limited accuracy in some cases. Employing fusion of multiple classifiers outputs into a single classification framework has been instrumental in achieving greater accuracy and performing automated big data analysis. The article proposes a bit fusion ensemble algorithm that minimizes the classification error rate and has been tested on various datasets. Five diversified base classifiers k- nearest neighbor (KNN), Support Vector Machine (SVM), Multi-Layer Perceptron (MLP), Decision Tree (D.T.), and Naïve Bayesian Classifier (N.B.), are used in the implementation model. Bit fusion algorithm works on the individual input from the classifiers. Decision vectors of the base classifier are weighted transformed into binary bits by comparing with high-reliability threshold parameters. The output of each base classifier is considered as soft class vectors (CV). These vectors are weighted, transformed and compared with a high threshold value of initialized δ = 0.9 for reliability. Binary patterns are extracted, and the model is trained and tested again. The standard fusion approach and proposed bit fusion algorithm have been compared by average error rate. The error rate of the Bit-fusion algorithm has been observed with the values 5.97, 12.6, 4.64, 0, 0, 27.28 for Leukemia, Breast cancer, Lung Cancer, Hepatitis, Lymphoma, Embryonal Tumors, respectively. The model is trained and tested over datasets from UCI, UEA, and UCR repositories as well which also have shown reduction in the error rates.
Alcohol and Health Outcomes: An Umbrella Review of Meta-Analyses Base on Prospective Cohort Studies
Authors: Zhong Lixian , Chen Weiwei , Wang Tonghua , Zeng Qiuting , Lai Leizhen , Lai Junlong , Lin Junqin , Tang Shaohui
Published: May 2022

An umbrella review of meta-analyses was performed to summarize the evidence of associations between alcohol consumption and health outcomes and to assess its credibility. Meta-analyses of prospective cohort studies reporting the associations of alcohol consumption with health outcomes were identified. We recalculated the random-effects summary effect size and 95% confidence interval, heterogeneity, and small-study effect for each meta-analysis and graded the evidence. Fifty-nine publications reporting 224 meta-analyses of prospective cohort studies with 140 unique health outcomes were included, in which there were 49 beneficial associations and 25 harmful associations with nominally statistically significant summary results. But quality of evidence was rated high only for seven beneficial associations (renal cell carcinoma risk, dementia risk, colorectal cancer mortality, and all-cause mortality in patients with hypertension for low alcohol consumption; renal cell carcinoma risk, cardiovascular disease (CVD) risk in patients with hypertension and all-cause mortality in patients with hypertension for moderate consumption) and four harmful associations (cutaneous basal cell carcinoma risk for low alcohol consumption; cutaneous basal cell carcinoma risk and cutaneous squamous cell carcinoma risk for moderate alcohol consumption; hemorrhagic stroke risk for high alcohol consumption). In this umbrella review, only 11 health outcomes (5 in low alcohol consumption, 5 in moderate alcohol consumption and 1 in high alcohol consumption) with statistically significant showed high quality of epidemiologic evidence. More robust and larger prospective studies are needed to verify our results.
Immunomodulatory Activities of Periodontal Ligament Stem Cells in Orthodontic Forces-Induced Inflammatory Processes: Current Views and Future Perspectives
Authors: Behm Christian , Zhao Zhongqi , Andrukhov Oleh
Published: May 2022

Orthodontic tooth movement (OTM) is induced by applying active mechanical forces, causing a local non-infectious inflammatory response in the periodontal ligament (PDL). As a prerequisite for OTM, the inflammation status is associated with increased levels of various cytokines and involves the interaction between immune cells and periodontal ligament stem cells (hPDLSCs). It is well established that hPDLSCs respond to orthodontic forces in several ways, such as by secreting multiple inflammatory factors. Another essential feature of hPDLSCs is their immunomodulatory activities, which are executed through cytokine (e.g., TNF-α and IL-1β)-induced production of various soluble immunomediators (e.g., indoleamine-2,3-dioxygenase-1, tumor necrosis factor-inducible gene 6 protein, prostaglandin E2) and direct cell-to-cell contact (e.g., programmed cell death ligand 1, programmed cell death ligand 2). It is well known that these immunomodulatory abilities are essential for local periodontal tissue homeostasis and regeneration. So far, only a handful of studies provides first hints that hPDLSCs change immunological processes during OTM via their immunomodulatory activities. These studies demonstrate the pro-inflammatory aspect of immunomodulation by hPDLSCs. However, no studies exist which investigate cytokine and cell-to-cell contact mediated immunomodulatory activities of hPDLSCs. In this perspective article, we will discuss the potential role of the immunomodulatory potential of hPDLSCs in establishing and resolving the OTM-associated non-infectious inflammation and hence its potential impact on periodontal tissue homeostasis during OTM.
Liver Transplantation for Hepatocellular Carcinoma With Bile Duct Tumor-Associated Thrombi: A Systematic Review and Pooled Analysis
Authors: Kim Steven C. , Bolognese Alexandra C. , Little Christopher J. , Hitchcock Mary E. , Leverson Glen E. , Al-Adra David P.
Published: May 2022

Introduction

The significance of bile duct tumor-associated thrombi in patients undergoing transplantation for hepatocellular carcinoma (HCC) is controversial. Therefore, we performed a systematic review of the literature with pooled analysis to investigate the impact of biliary invasion on HCC recurrence and patient survival.

Methods

Of 1,584 references screened, eight were included for analysis. Demographics, patient and tumor factors, recurrence, and survival data were analyzed. Time to recurrence and death were extracted from each paper by cross-referencing survival curves.

Results

A total of 35 patients across eight studies were pooled for analysis when follow-up data were available. At 1 year, 92.9% of patients undergoing transplantation for HCC with bile duct thrombi were alive. Overall survival at 3 and 5 years was 65.5 and 49.6%, respectively. At 1 year, 21.6% of patients had recurrence of their disease, while at 3 years, 50.4% of patients had recurrence. Of those patients with recurrence in the first year, 71.4% recurred within the first 3 months after transplantation.

Conclusion

Overall patient survival decreased within the first 5 years, but then stabilized. The 5-year survival rate of 49.6% in this pooled analysis is lower than that reported for patients undergoing transplantation for HCC within the Milan criteria (50–78%) or recent reports in patients with portal vein involvement (63.6%), though data is limited by a lack of long-term follow-up in this understudied population. Transplantation for patients with HCC with bile duct involvement may be a viable treatment option, warranting further investigation.
The Solar Eclipse That Validated Einstein’s Theory of Relativity
Authors: Gutfreund Hanoch
Published: May 2022

This article describes one of the most important chapters in scientific history, which contributed to the modern understanding of the universe. Albert Einstein completed his theory of general relativity in 1915. This theory changed our understanding of the concepts of space, time and gravity, established by Isaac Newton. One prediction of the new theory was that light rays reaching us from distant stars, curve when they pass near the sun, because of the sun’s gravity and the nature of space and time. This prediction was confirmed through astronomical observations measuring the light reaching Earth from distant stars. When this result was published, it ignited a big excitement and interest in the scientific community and the general public, and Einstein became a superstar overnight.
Influence of Psychological Nursing Procedure on Negative Emotion, Stress State, Quality of Life and Nursing Satisfaction in Patients with Lung Cancer Radical Operation
Authors: Yu Yizhi , Xia You , Fan Xiaoyan , Chen Yong , Li Chengjuan , Zhang Jing
Published: May 2022

Objective

To discuss the influence of psychological nursing procedure on negative emotion, stress state, quality of life and nursing satisfaction in patients with lung cancer radical operation.

Methods

106 patients with lung cancer who underwent radical resection in our hospital from September 2019 to September 2021 were selected. According to the intervention time, patients were divided into Group A and Group B, with 53 cases in each group. Group A received routine nursing, Group B used psychological nursing procedure on the basis of Group A. The negative emotions, stress state, quality of life and nursing satisfaction of patient were observed.

Results

Self-rating anxiety scale and self-rating depression scale scores of Group B were lower than Group A (P < 0.05). The levels of norepinephrine, epinephrine and cortisol in Group B were lower than Group A (P < 0.05). Generic quality of life inventory-74 scores of Group B were higher than Group A (P < 0.05). The nursing satisfaction of Group B (88.68%) was higher than Group A (73.58%) (P < 0.05).

Conclusion

Psychological nursing procedure is conducive to reducing the negative emotion, relieving stress reaction, improving the quality of life, increasing nursing satisfaction of patients with lung cancer radical operation.
Antimicrobial Resistance in Bacteria Isolated From Canine Urine Samples Submitted to a Veterinary Diagnostic Laboratory, Illinois, United States
Authors: Yudhanto Setyo , Hung Chien-Che , Maddox Carol W. , Varga Csaba
Published: May 2022

The emergence of antimicrobial resistance (AMR) in dogs constitutes a threat to animal and human health. There is a lack of studies in Illinois that evaluated the prevalence of AMR among urinary bacterial pathogens. In the study, we included 803 isolates (299 Gram-positive and 504 Gram-negative) that were isolated from 2,583 canine urine samples submitted to the Veterinary Diagnostic Laboratory, the University of Illinois between 2019 and 2020 from dogs suspected of urinary tract infections (UTI). The most common Gram-positive isolates included Staphylococcus pseudintermedius (17.93%), Enterococcus faecalis (9.46%), Streptococcus canis (6.10%), and Enterococcus faecium (3.74%), while Gram-negative isolates included Escherichia coli (45.58%), Proteus mirabilis (11.08%), Klebsiella pneumoniae (3.11%), and Pseudomonas aeruginosa (2.99%). Among the Gram-positive isolates, Staphylococcus pseudintermedius isolates showed a very high prevalence of resistance to penicillin (56.94%), a high prevalence of resistance to trimethoprim-sulfamethoxazole (31.94%), enrofloxacin (29.17%), and oxacillin (27.08%). Among Gram-negative bacteria, Escherichia coli isolates showed a high prevalence of resistance to ampicillin (31.42%). Considering the high prevalence of resistance to antimicrobials commonly used to treat UTI in dogs, urine samples should be collected for bacterial culture and susceptibility testing before treatment initiation to prevent treatment failures and the development of multidrug resistance. Given the possibility of zoonotic transmission of antimicrobial-resistant bacteria, veterinarians when treating UTI cases, should inform dog owners of the potential transmission risk.
Associations of COVID-19-Related Health, Healthcare and Economic Factors With Prenatal Depression and Anxiety
Authors: Avalos Lyndsay A. , Nance Nerissa , Badon Sylvia E. , Young-Wolff Kelly , Ames Jennifer , Zhu Yeyi , Hedderson Monique M. , Ferrara Assiamira , Zerbo Ousseny , Greenberg Mara , Croen Lisa A.
Published: May 2022

Objective: This study evaluated whether COVID-19 pandemic-related health, healthcare and economic factors during pregnancy are associated with prenatal depression and anxiety.

Methods: We conducted a cross-sectional study of 6,628 pregnant members of Kaiser Permanente Northern California who responded to a survey between 22 June and 30 September 2020. The survey included questions about depression (Patient Health Questionnaire) and anxiety (Generalized Anxiety Disorder) symptoms and COVID-19-related health and healthcare (e.g., had COVID-19) and economic (e.g., food insecurity) factors.

Results: Over one third of individuals reported depression (25% mild, 8% moderate, 3% severe) or anxiety (22% mild, 8% moderate, 5% severe) symptoms. In multivariable analyses, COVID-19 during pregnancy, employment with greater risk of COVID-19, distress over changes in prenatal care, job loss, changes in childcare and food insecurity were associated with greater odds of prenatal depression or anxiety.

Conclusion: Findings suggest the COVID-19 pandemic may have severe mental health repercussions for pregnant individuals. Support services for pregnant individuals experiencing these COVID-19-related factors and monitoring of those who had moderate/severe prenatal depression and anxiety symptoms during the COVID-19 pandemic is warranted.
Mechanism of Reduction of Drought-Induced Oxidative Stress in Maize Plants by Fertilizer Seed Coating
Authors: Matłok Natalia , Piechowiak Tomasz , Królikowski Kamil , Balawejder Maciej
Published: May 2022

In this study, we investigated the effects of the utilization of seed coating by dedicated fertilizer on the mechanism of oxidative stress reduction in maize growing in simulated drought conditions. A strict pot experiment was conducted for this purpose in a climatic chamber with a phytotron system and controlled temperature and air humidity. Maize seeds were planted and grown in soil with 20% (extreme drought), 40%, and 60% water holding capacity (WHC). The seeds were enhanced using proposed fertilizer and applied at a rate of 2 kg t−1 seeds. The levels of ROS, as well as antioxidant enzymes SOD, CAT, and GPOX, generated by plants enhanced using the seed coating by fertilizer and by control specimens (non-enhanced), were measured 21 days after the seeds were planted. Antioxidant potential and total polyphenol contents in the plants were also determined. The findings show that under drought stress, plants produce high levels of ROS, which is responsible for oxidative stress. However, the latter phenomenon may be reduced using seed coating. Application of seed coating by fertilizer contributed to a 32.7% decrease in ROS in the case of extreme drought (soil with 20% WHC). The treatment also led to increased activity of SOD (61.2%), CAT (45.7%), and GPOX (35.8%), which shows its positive effects on activation of the enzymatic antioxidant system responsible for neutralization of ROS and for reducing the negative effects of drought.
The Genetic Assessment of South African Nguni Sheep Breeds Using the Ovine 50K Chip
Authors: Nxumalo Khanyisani S. , Grobler Paul , Ehlers Karen , Nesengani Lucky T. , Mapholi Ntanganedzeni O.
Published: May 2022

The indigenous sheep breeds of South Africa, such as the Nguni, are well adapted to different ecological regions throughout the country. This has resulted into different ecotypes. However, it is not clear if the differences among Nguni sheep are genetically distinct. The present study aimed to use the latest technology to assess the genetic relationship between Nguni sheep ecotypes and the relationship to other selected South African breeds using SNP markers. In the current study, 144 South African sheep samples (75 Nguni sheep and 69 mixed-breed sheep as a reference) were genotyped using the OvineSNP50 Bead Chip assay from Illumina. The Nguni consisted of 25 Pedi, 25 Swazi, and 25 Zulu sheep, with the reference group comprising 25 Namaqua, 23 Dorper, and 21 Damara sheep. After quality control of 54,241 SNPs, 48,429 SNPs remained for analysis (MAF > 0.05). There were genetic differences in the Nguni sheep population; notably, the Zulu and Swazi populations clustered together, but with a clear distinction from the Pedi ecotype. Genetic admixtures were detected in the Damara and Dorper sheep. This is most likely a consequence of recent intermixing between indigenous and commercial breeds. The levels of genetic diversity within individual types were generally lower compared to commercial breeds. This study revealed an understanding regarding genetic variation within and among indigenous sheep breeds, which can be used as baseline information for establishing conservation and breeding programmes.
Long-Term CBCT Evaluation of Mandibular Third Molar Changes after Distalization in Adolescents
Authors: Hong Hye-Rim , Lee Nam-Ki , Park Jae Hyun , Ku Ja Hyeong , Kim Jaehyun , Bayome Mohamed , Mo Sung-Seo , Kook Yoon-Ah
Published: May 2022

Introduction: This study aimed to evaluate mandibular molar changes after distalization using Class III elastics and modified C-palatal plates (MCPPs) in the maxilla and to use CBCT to specifically analyze the third-molar position after long-term retention in adolescents. Methods: The sample consisted of 65 mandibular third molars from 35 subjects, divided into two groups. Twenty-six of the third molars were from fourteen adolescent patients with Class I bimaxillary protrusion (mean age, 12.8 years) who had undergone bilateral maxillary distalization using an MCPP and mandibular distalization with Class III elastics with non-extraction treatment. For the control group, 39 third molars from 21 Class I normal-occlusion subjects were used. In the treated group, CBCT images were taken before distalization (T1), after molar distalization (T2), and at a long-term observation point (6.1 ± 2.7 years) (T3). Repeated measures ANOVA and a post hoc test with Bonferroni correction were used to analyze significant differences in the positions at the three points in time. Results: There were 2.3 and 1.7 mm of distal movement at the crown of the mandibular first and second molars, with distal tipping of 5.2° and 5.3°, respectively, and 3.0° of distal tipping of the third molars. Comparing the treated group with the control group at T3, there was a significant change in the angulation of the third molars (p < 0.001). In the long-term, the third molars from the treated group showed a downward and buccal position after distalization. Conclusions: In the long term, the developing mandibular third molar buds had downward and buccal positional changes after total arch distalization. These findings suggest the need for clinicians to consider the possibility of the impaction of developing third molars after mandibular total arch distalization in adolescents.
Extracellular Vesicles in the Progression and Therapeutic Resistance of Nasopharyngeal Carcinoma
Authors: Shan Yunhan , Zhou Peijun , Zhou Qin , Yang Lifang
Published: May 2022

Nasopharyngeal carcinoma (NPC) is an epithelial malignancy largely associated with Epstein–Barr virus (EBV) infection, which is frequently reported in east and southeast Asia. Extracellular vesicles (EVs) originate from the endosome or plasma membrane, which plays a critical role in tumor pathogenesis for their character of cell-cell communication and its cargos, including proteins, RNA, and other molecules that can target recipient cells and affect their progression. To date, numerous studies have indicated that EVs have crucial significance in the progression, metastasis, and therapeutic resistance of NPC. In this review, we not only summarize the interaction of NPC cells and the tumor microenvironment (TME) through EVs, but also explain the role of EVs in radiation and drug resistance of NPC, which poses a severe threat to cancer therapy. Therefore, EVs may show great potential as biomarkers in the early diagnosis of interfered targets of NPC therapy.
Lipocalin 2 Deficiency Alters Prostaglandin Biosynthesis and mTOR Signaling Regulation of Thermogenesis and Lipid Metabolism in Adipocytes
Authors: Deis Jessica , Lin Te-Yueh , Bushman Theresa , Chen Xiaoli
Published: May 2022

Apart from a well-known role in the innate immune system, lipocalin 2 (Lcn2) has been recently characterized as a critical regulator of thermogenesis and lipid metabolism. However, the physiological mechanism through which Lcn2 regulates cellular metabolism and thermogenesis in adipocytes remains unknown. We found that Lcn2 expression and secretion are significantly upregulated by arachidonic acid (AA) and mTORC1 inhibition in differentiated inguinal adipocytes. AA-induced Lcn2 expression and secretion correlate with the inflammatory NFkB activation. Lcn2 deficiency leads to the upregulation of cyclooxygenase-2 (COX2) expression, as well as increased biosynthesis and secretion of prostaglandins (PGs), particularly PGE2 and PGD2, induced by AA in adipocytes. Furthermore, Lcn2 deficiency affects the mTOR signaling regulation of thermogenic gene expression, lipogenesis, and lipolysis. The loss of Lcn2 dismisses the effect of mTORC1 inhibition by rapamycin on COX2, thermogenesis genes, lipogenesis, and lipolysis, but has no impact on p70 S6Kinase-ULK1 activation in Lcn2-deficient adipocytes. We conclude that Lcn2 converges the COX2-PGE2 and mTOR signaling pathways in the regulation of thermogenesis and lipid metabolism in adipocytes.
Effect of Solid-Solid Phase Change Material’s Direct Interaction on Physical and Rheological Properties of Asphalt
Authors: Zhao Haisheng , Guo Jianmin , Ma Shijie , Zhang Huan , Su Chunhua , Wang Xiaoyan , Li Zengguang , Wei Jincheng , Cui Shiping
Published: May 2022

Asphalt pavement is a temperature−sensitive structure that is prone to temperature-related diseases. Phase change material (PCM) is an excellent candidate for mitigating these diseases. This paper looked into the effects of indirect composite shape-stabilized PCM incorporation on the characteristics of asphalt. The compatibility, physical properties, and rheological properties of asphalt with various PCM content before and after aging were thoroughly investigated. No phase separation and no chemical reaction occurred between PCM and asphalt. The physical properties improved with the addition of PCM, and the high−temperature performance indexes improved while the low−temperature performance indexes decreased as the aging process progressed. The effects of PCM on the rheological properties of the matrix and SBS−modified asphalt was distinct. PCM was added to improve the high−temperature rheological characteristics of the matrix asphalt when the temperature was higher than 52 °C, while PCM reduced the high−temperature rheological properties of the SBS−modified asphalt. The aging process has an impact on the high−temperature rutting factor of asphalt with a high PCM content. The low−temperature creep behavior and PG grade of asphalt were both improved. The implication of PCM is that it cannot increase the thermoregulation of asphalt pavement without the cost of scarifying the performance of the asphalt or mixture.
Reaction Sintering of MgAlON at 1500 °C from Al2O3, MgO and AlN and Its Wettability by AlSi7Mg
Authors: Schramm Alina , Thümmler Martin , Fabrichnaya Olga , Brehm Simon , Kraus Jakob , Kortus Jens , Rafaja David , Scharf Christiane , Aneziris Christos G.
Published: May 2022

The aim of this study is the investigation of a technological synthesis of MgAlON, which is a prospective coating material on ceramic foam filters for the filtration of magnesium, aluminum, and other metal melts. Thermodynamic calculations are performed, and the synthesis is carried out at 1500 °C in nitrogen atmosphere using samples consisting of different fractions of Al2O3, MgO, and AlN as starting materials. The effect of the quantity of these components on the conversion degree of the educts is evaluated. Furthermore, the effect of the holding time at the synthesis temperature, as well as the composition points or regions showing the highest conversion degree, are determined. XRD analysis is performed to evaluate the phase fractions and lattice parameters of the spinel after the respective reaction, and the nitrogen content of selected samples is evaluated. Sessile drop tests using AlSi7Mg are performed at 950 °C on selected sintered samples, determining their wettability, and therefore, applicability of the material in light metal melt filtration.
Fingerprints of Topotactic Hydrogen in Nickelate Superconductors
Authors: Si Liang , Worm Paul , Held Karsten
Published: May 2022

Superconductivity has entered the nickel age marked by enormous experimental and theoretical efforts. Notwithstanding, synthesizing nickelate superconductors remains extremely challenging, not least due to incomplete oxygen reduction and topotactic hydrogen. Here, we present density-functional theory calculations for nickelate superconductors with additional topotactic hydrogen or oxygen, namely La1−xSrxNiO2Hδ and LaNiO2+δ. We identify a phonon mode as a possible indication for topotactic hydrogen and discuss the charge redistribution patterns around oxygen and hydrogen impurities.
Invasive Fungal Disease in Patients with Chronic Lymphocytic Leukemia in Japan: A Retrospective Database Study
Authors: Yasu Takeo , Sakurai Kotono , Akazawa Manabu
Published: May 2022

Invasive fungal disease (IFD) is an important cause of morbidity and mortality in patients with hematological malignancies. As chronic lymphocytic leukemia (CLL) is a rare hematological malignancy in Japan, IFD incidence in Japanese patients with CLL is unclear. This study aimed to investigate IFD incidence in Japanese patients with CLL. This retrospective cohort study used data of patients with CLL registered between April 2008 and December 2019 in the Medical Data Vision database (n = 3484). IFD incidence after CLL diagnosis in the watch-and-wait (WW) and drug therapy (DT) groups was 1.5% and 9.2%, respectively. The most common type of IFD was invasive aspergillosis (28.1%). Cox proportional hazards multivariate analysis revealed that DT (hazard ratio [HR]: 2.13) and steroid use (HR: 4.19) were significantly associated with IFD occurrence. IFD incidence was significantly higher in the DT group than in the WW group (log-rank p < 0.001); however, there was no significant between-group difference in the time to IFD onset or the type of IFD (p = 0.09). This study determined the incidence of IFD in patients with CLL during WW. Physicians should monitor for IFD, even among patients with CLL undergoing the WW protocol.
Method for the Intraoperative Detection of IDH Mutation in Gliomas with Differential Mobility Spectrometry
Authors: Haapala Ilkka , Rauhameri Anton , Roine Antti , Mäkelä Meri , Kontunen Anton , Karjalainen Markus , Laakso Aki , Koroknay-Pál Päivi , Nordfors Kristiina , Haapasalo Hannu , Oksala Niku , Vehkaoja Antti , Haapasalo Joonas
Published: May 2022

Isocitrate dehydrogenase (IDH) mutation status is an important factor for surgical decision-making: patients with IDH-mutated tumors are more likely to have a good long-term prognosis, and thus favor aggressive resection with more survival benefit to gain. Patients with IDH wild-type tumors have generally poorer prognosis and, therefore, conservative resection to avoid neurological deficit is favored. Current histopathological analysis with frozen sections is unable to identify IDH mutation status intraoperatively, and more advanced methods are therefore needed. We examined a novel method suitable for intraoperative IDH mutation identification that is based on the differential mobility spectrometry (DMS) analysis of the tumor. We prospectively obtained tumor samples from 22 patients, including 11 IDH-mutated and 11 IDH wild-type tumors. The tumors were cut in 88 smaller specimens that were analyzed with DMS. With a linear discriminant analysis (LDA) algorithm, the DMS was able to classify tumor samples with 86% classification accuracy, 86% sensitivity, and 85% specificity. Our results show that DMS is able to differentiate IDH-mutated and IDH wild-type tumors with good accuracy in a setting suitable for intraoperative use, which makes it a promising novel solution for neurosurgical practice.