bluuebunny/crossref_metadata_2025 huggingface dataset:
New insight into the granule formation in the reactor for enhanced biological phosphorus removal
Authors: Pelevina Anna , Gruzdev Evgeny , Berestovskaya Yulia , Dorofeev Alexander , Nikolaev Yury , Kallistova Anna , Beletsky Alexey , Ravin Nikolai , Pimenov Nikolai , Mardanov Andrey
Published: December 2023

While granulated activated sludge exhibits high productivity, the processes of granule formation are incompletely studied. The processes of granule formation and succession of communities were investigated in a laboratory sequencing batch reactor (SBR) under conditions for enhanced biological phosphorus removal (EBPR) using microbiological and molecular techniques. Active consumption of acetate, primarily by the phosphate-accumulating organisms (PAO), commenced at day 150 of cultivation. This was indicated by the high ratio of molar P-released/acetate uptake (0.73–0.77 P-mol/C-mol), characteristic of PAO. During this period, two types of granule-like aggregates formed spontaneously out of the activated sludge flocs. The aggregates differed in morphology and microbial taxonomic composition. While both aggregate types contained phosphorus-enriched bacterial cells, PAO prevailed in those of morphotype I, and glycogen-accumulating organisms (GAOs) were predominant in the aggregates of morphotype II. After 250 days, the elimination of the morphotype II aggregates from the reactor was observed. The subsequent selection of the community was associated with the development of the morphotype I aggregates, in which the relative abundance of PAO increased significantly, resulting in higher efficiency of phosphorus removal. Metagenomic analysis revealed a predominance of the organisms closely related toCandidatusAccumulibacter IС and IIС and ofCa.Accumulibacter IIB among the PAO. Based on the content of the genes of the key metabolic pathways, the genomes of potential PAO belonging to the generaAmaricoccus, Azonexus, Thauera, Zoogloea, Pinisolibacter, andSiculibacilluswere selected. The patterns of physicochemical processes and the microbiome structure associated with granule formation and succession of the microbial communities were revealed.
Antibiotic resistance profiles of gut microbiota across various primate species in Guangxi
Authors: Huang Hongli , Pang Xianwu , Que Tengcheng , Chen Panyu , Li Shousheng , Wu Aiqiong , He Meihong , Qiu Hong , Hu Yanling
Published: December 2023

Introduction

Understanding the gut microbiota and antibiotic resistance gene (ARG) profiles in non-human primates (NHPs) is crucial for evaluating their potential impact on human health and the environment.

Methods

In this study, we performed metagenomic analysis of 203 primate fecal samples, including nine NHP species and humans, to comprehensively characterize their gut microbiota and ARGs.

Results

Our study reveals the prevailing phyla in primates as Firmicutes, Bacteroidetes, Euryarchaeota, and Proteobacteria. The captive NHPs exhibited higher ARG abundance compared to their wild counterparts, with tetracycline and beta-lactam resistance genes prevailing. Notably, ARG subtypes in Trachypithecus leucocephalus (T. leucocephalus) residing in karst limestone habitats displayed a more dispersed distribution compared to other species. Interestingly, ARG profiles of NHPs clustered based on geographic location and captivity status. Co-occurrence network analysis revealed intricate correlations between ARG subtypes and bacterial taxa. Procrustes analysis unveiled a significant correlation between ARGs and microbial phylogenetic community structure. Taxonomic composition analysis further highlighted differences in microbial abundance among NHPs and humans.

Discussion

Our study underscores the impact of lifestyle and geographical location on NHP gut microbiota and ARGs, providing essential insights into the potential risks posed by NHPs to antibiotic resistance dissemination. This comprehensive analysis enhances our understanding of the interplay between NHPs and the gut resistome, offering a critical reference for future research on antibiotic resistance and host-microbe interactions.
An efficient model for predicting human diseases through miRNA based on multiple-types of contrastive learning
Authors: Liao Qingquan , Fu Xiangzheng , Zhuo Linlin , Chen Hao
Published: December 2023

Multiple studies have demonstrated that microRNA (miRNA) can be deeply involved in the regulatory mechanism of human microbiota, thereby inducing disease. Developing effective methods to infer potential associations between microRNAs (miRNAs) and diseases can aid early diagnosis and treatment. Recent methods utilize machine learning or deep learning to predict miRNA-disease associations (MDAs), achieving state-of-the-art performance. However, the problem of sparse neighborhoods of nodes due to lack of data has not been well solved. To this end, we propose a new model named MTCL-MDA, which integrates multiple-types of contrastive learning strategies into a graph collaborative filtering model to predict potential MDAs. The model adopts a contrastive learning strategy based on topology, which alleviates the damage to model performance caused by sparse neighborhoods. In addition, the model also adopts a semantic-based contrastive learning strategy, which not only reduces the impact of noise introduced by topology-based contrastive learning, but also enhances the semantic information of nodes. Experimental results show that our model outperforms existing models on all evaluation metrics. Case analysis shows that our model can more accurately identify potential MDA, which is of great significance for the screening and diagnosis of real-life diseases. Our data and code are publicly available at: https://github.com/Lqingquan/MTCL-MDA.
Association between the triglyceride glucose index and cognitive impairment and dementia: a meta-analysis
Authors: Wang Huan , Ling Qin , Wu Yifan , Zhang Mingjie
Published: December 2023

Background

The triglyceride and glucose (TyG) index is an alternative index of insulin resistance (IR). We aimed to clarify the relationship between the TyG index and cognitive impairment and dementia.

Methods

We conducted a comprehensive search of the PubMed, Cochrane Library, and Embase databases until February 2023 to identify relevant studies. Random-effects models were used to pool effect sizes, and the Grading of Recommendations Assessment, Development, and Evaluation system (GRADE) was used to assess the quality of the evidence.

Results

Ten studies were included, with seven of which investigated the relationship between the TyG index and cognitive impairment and three exploring the association between the TyG index and dementia. When the TyG index was described as a categorical variable, it was positively associated with the risk of cognitive impairment (OR = 2.32; 95% CI 1.39–3.87) and dementia (OR = 1.14, 95% CI 1.12–1.16). The association of the TyG index with the risk of cognitive impairment (OR = 3.39, 95% CI 1.67–6.84) and dementia (OR = 1.37, 95% CI 1.03–1.83) remained significant for per 1 unit increment in the TyG index. The GRADE assessment indicated a very low certainty for cognitive impairment. Low certainty and moderate certainty were observed for dementia when the TyG index was analyzed as a categorical variable and as a continuous variable, respectively.

Conclusion

The TyG index is associated with an increased risk of cognitive impairment and dementia. Further prospective research is warranted to confirm these findings.

Systematic review registration: https://www.crd.york.ac.uk/, Protocol registration number: CRD42023388028.
Ventromedial hypothalamus relays chronic stress inputs and exerts bidirectional regulation on anxiety state and related sympathetic activity
Authors: Shao Jie , Chen Yan , Gao Dashuang , Liu Yunhui , Hu Nan , Yin Lianghong , Zhang Xinzhou , Yang Fan
Published: December 2023

Chronic stress can induce negative emotion states, including anxiety and depression, leading to sympathetic overactivation and disturbed physiological homeostasis in peripheral tissues. While anxiety-related neural circuitry integrates chronic stress information and modulates sympathetic nervous system (SNS) activity, the critical nodes linking anxiety and sympathetic activity still need to be clarified. In our previous study, we demonstrated that the ventromedial hypothalamus (VMH) is involved in integrating chronic stress inputs and exerting influence on sympathetic activity. However, the underlying synaptic and electrophysiological mechanisms remain elusive. In this study, we combined in vitro electrophysiological recordings, behavioral tests, optogenetic manipulations, and SNS activity analyses to explore the role of VMH in linking anxiety emotion and peripheral SNS activity. Results showed that the VMH played an important role in bidirectionally regulating anxiety-like behavior and peripheral sympathetic excitation. Chronic stress enhanced excitatory inputs into VMH neurons by strengthening the connection with the paraventricular hypothalamus (PVN), hence promoting anxiety and sympathetic tone outflow, an important factor contributing to the development of metabolic imbalance in peripheral tissues and cardiovascular diseases.
A modelling study to dissect the potential role of voltage-gated ion channels in activity-dependent conduction velocity changes as identified in small fiber neuropathy patients
Authors: Maxion Anna , Kutafina Ekaterina , Dohrn Maike F. , Sacré Pierre , Lampert Angelika , Tigerholm Jenny , Namer Barbara
Published: December 2023

Objective

Patients with small fiber neuropathy (SFN) suffer from neuropathic pain, which is still a therapeutic problem. Changed activation patterns of mechano-insensitive peripheral nerve fibers (CMi) could cause neuropathic pain. However, there is sparse knowledge about mechanisms leading to CMi dysfunction since it is difficult to dissect specific molecular mechanisms in humans. We used an in-silico model to elucidate molecular causes of CMi dysfunction as observed in single nerve fiber recordings (microneurography) of SFN patients.

Approach

We analyzed microneurography data from 97 CMi-fibers from healthy individuals and 34 of SFN patients to identify activity-dependent changes in conduction velocity. Using the NEURON environment, we adapted a biophysical realistic preexisting CMi-fiber model with ion channels described by Hodgkin-Huxley dynamics for identifying molecular mechanisms leading to those changes. Via a grid search optimization, we assessed the interplay between different ion channels, Na-K-pump, and resting membrane potential.

Main results

Changing a single ion channel conductance, Na-K-pump or membrane potential individually is not sufficient to reproduce in-silico CMi-fiber dysfunction of unchanged activity-dependent conduction velocity slowing and quicker normalization of conduction velocity after stimulation as observed in microneurography. We identified the best combination of mechanisms: increased conductance of potassium delayed-rectifier and decreased conductance of Na-K-pump and depolarized membrane potential. When the membrane potential is unchanged, opposite changes in Na-K-pump and ion channels generate the same effect.

Significance

Our study suggests that not one single mechanism accounts for pain-relevant changes in CMi-fibers, but a combination of mechanisms. A depolarized membrane potential, as previously observed in patients with neuropathic pain, leads to changes in the contribution of ion channels and the Na-K-pump. Thus, when searching for targets for the treatment of neuropathic pain, combinations of several molecules in interplay with the membrane potential should be regarded.
Factor analysis based on SHapley Additive exPlanations for sepsis-associated encephalopathy in ICU mortality prediction using XGBoost — a retrospective study based on two large database
Authors: Guo Jiayu , Cheng Hongtao , Wang Zicheng , Qiao Mengmeng , Li Jing , Lyu Jun
Published: December 2023

Objective

Sepsis-associated encephalopathy (SAE) is strongly linked to a high mortality risk, and frequently occurs in conjunction with the acute and late phases of sepsis. The objective of this study was to construct and verify a predictive model for mortality in ICU-dwelling patients with SAE.

Methods

The study selected 7,576 patients with SAE from the MIMIC-IV database according to the inclusion criteria and randomly divided them into training (n = 5,303, 70%) and internal validation (n = 2,273, 30%) sets. According to the same criteria, 1,573 patients from the eICU-CRD database were included as an external test set. Independent risk factors for ICU mortality were identified using Extreme Gradient Boosting (XGBoost) software, and prediction models were constructed and verified using the validation set. The receiver operating characteristic (ROC) and the area under the ROC curve (AUC) were used to evaluate the discrimination ability of the model. The SHapley Additive exPlanations (SHAP) approach was applied to determine the Shapley values for specific patients, account for the effects of factors attributed to the model, and examine how specific traits affect the output of the model.

Results

The survival rate of patients with SAE in the MIMIC-IV database was 88.6% and that of 1,573 patients in the eICU-CRD database was 89.1%. The ROC of the XGBoost model indicated good discrimination. The AUCs for the training, test, and validation sets were 0.908, 0.898, and 0.778, respectively. The impact of each parameter on the XGBoost model was depicted using a SHAP plot, covering both positive (acute physiology score III, vasopressin, age, red blood cell distribution width, partial thromboplastin time, and norepinephrine) and negative (Glasgow Coma Scale) ones.

Conclusion

A prediction model developed using XGBoost can accurately predict the ICU mortality of patients with SAE. The SHAP approach can enhance the interpretability of the machine-learning model and support clinical decision-making.
Stroke classification and treatment support system artificial intelligence for usefulness of stroke diagnosis
Authors: Miyamoto Nobukazu , Ueno Yuji , Yamashiro Kazuo , Hira Kenichiro , Kijima Chikage , Kitora Naoki , Iwao Yoshihiko , Okuda Kayo , Mishima Shohei , Takahashi Daisuke , Ono Kazuto , Asari Mika , Miyazaki Kazuki , Hattori Nobutaka
Published: December 2023

Background and aims

It is important to diagnose cerebral infarction at an early stage and select an appropriate treatment method. The number of stroke-trained physicians is unevenly distributed; thus, a shortage of specialists is a major problem in some regions. In this retrospective design study, we tested whether an artificial intelligence (AI) we built using computer-aided detection/diagnosis may help medical physicians to classify stroke for the appropriate treatment.

Methods

To build the Stroke Classification and Treatment Support System AI, the clinical data of 231 hospitalized patients with ischemic stroke from January 2016 to December 2017 were used for training the AI. To verify the diagnostic accuracy, 151 patients who were admitted for stroke between January 2018 and December 2018 were also enrolled.

Results

By utilizing multimodal data, such as DWI and ADC map images, as well as patient examination data, we were able to construct an AI that can explain the analysis results with a small amount of training data. Furthermore, the AI was able to classify with high accuracy (Cohort 1, evaluation data 88.7%; Cohort 2, validation data 86.1%).

Conclusion

In recent years, the treatment options for cerebral infarction have increased in number and complexity, making it even more important to provide appropriate treatment according to the initial diagnosis. This system could be used for initial treatment to automatically diagnose and classify strokes in hospitals where stroke-trained physicians are not available and improve the prognosis of cerebral infarction.
Serum levels of Vanin-2 increase with obesity in relation to inflammation of adipose tissue and may be a predictor of bariatric surgery outcomes
Authors: Geng Shan , Chen Dongmei , Wang Yanping , Yu Xingrui , Zuo Dan , Lv Xinlu , Zhou Xuelian , Hu Chengju , Yang Xuesong , Ma Xujue , Hu Wenjing , Xi Jiazhuang , Yu Shaohong
Published: December 2023

Objective

Excessive obesity can lead to dysfunction in adipose tissue, which contributes to the development of comorbidities associated with obesity, such as type 2 diabetes (T2D), cardiovascular and cerebrovascular disease, among others. Previous research has mainly focused on the Vanin family in systemic inflammatory diseases or predicting its role in tumor prognosis, while neglecting its role as a secretory protein in adipose tissue inflammation and metabolism. The objective of this study was to compare the changes in Vanin-2 levels in the circulating blood of normal and obese individuals, and to assess its correlation with inflammatory factors in vivo. Furthermore, the study aimed to systematically evaluate its effectiveness in human weight loss surgery.

Methods

Serum concentrations of Vanin-2 and inflammatory indicators were measured in 518 volunteers. Furthermore, the concentrations of Vanin-2 were measured both before and after weight loss through a dietetic program or laparoscopic sleeve gastrectomy (LSG). Additionally, we assessed the levels of insulin, adiponectin, and inflammation-related factors. The hormonal profile and changes in body weight were evaluated at baseline and 3 months after surgery.

Results

Serum levels of Vanin-2 were found to be significantly increased in individuals with overweight/obesity (OW/OB) group (controls 438.98 ± 72.44, OW/OB 530.89 ± 79.39 ug/L; p < 0.001). These increased levels were associated with IL-18, BMI, FAT%, and HOMA-IR. However, levels of Vanin-2 remained unchanged after conventional dietary treatment. On the other hand, weight loss induced by LSG resulted in a significant decrease in Vanin-2 concentrations from 586.44 ± 48.84 to 477.67 ± 30.27 ug/L (p < 0.001), and this decrease was associated with the Vanin-2 concentrations observed before the operation.

Conclusion

Serum Vanin-2 is a highly effective biomarker for assessing adipose tissue inflammation in obesity and has the potential to serve as a predictor of bariatric surgery outcomes.
There was a similar U-shaped nonlinear association between waist-to-height ratio and the risk of new-onset hypertension: findings from the CHNS
Authors: Wang Zhenwei , Shi Qian , Yan Xuejiao , Tang Junnan , Zhang Jinying
Published: December 2023

Background

The association between waist-to-height ratio (WHtR) with hypertension has not been adequately explained, so in this study we sought to clarify the predictive role of WHtR on the incidence of hypertension as well as the potential nonlinear associations in the general population.

Methods

In this large prospective cohort study, a total of 4,458 individuals from the China Health and Nutrition Survey (CHNS) were included in the analysis. Multivariate Cox regression analyses, subgroup analyses, receiver operator characteristic (ROC) and restricted cubic spline (RCS) analyses were used to examine the association of WHtR with the risk of new-onset hypertension.

Results

Hypertension occurred in 32.8% of participants during the maximum six-year follow-up period. Compared with the group with lower WHtR, the group with higher WHtR had a higher incidence of hypertension (p < 0.001). Multivariate Cox regression analysis showed that the risk of hypertension was 1.45 times higher in the high WHtR group than in the low WHtR group, and that the risk of hypertension increased by 30.4% for every 0.1 unit increase in WHtR (p < 0.001). Subgroup analyses also validated the stratified associations between WHtR and the risk of new-onset hypertension in most subgroups (p < 0.05). ROC analyses also revealed that WHtR was superior to body mass index in predicting new-onset hypertension (AUC: 0.626 vs. 0.607, p = 0.009). Further RCS analysis detected a nonlinear association between WHtR and risk of new-onset hypertension (P for nonlinearity <0.001).

Conclusion

WHtR was nonlinearly associated with the risk of new-onset hypertension in the general population.
Chromosomal dynamics in Senna: comparative PLOP–FISH analysis of tandem repeats and flow cytometric nuclear genome size estimations
Authors: Nguyen Thi Hong , Kang Byung Yong , Kim Hyun Hee
Published: December 2023

Introduction

Tandem repeats (TRs) occur abundantly in plant genomes. They play essential roles that affect genome organization and evolution by inducing or generating chromosomal rearrangements such as duplications, deletions, inversions, and translocations. These impact gene expression and chromosome structure and even contribute to the emergence of new species.

Method

We investigated the effects of TRs on speciation in Senna genus by performing a comparative analysis using fluorescence in situ hybridization (FISH) with S. tora-specific TR probes. We examined the chromosomal distribution of these TRs and compared the genome sizes of seven Senna species (estimated using flow cytometry) to better understand their evolutionary relationships.

Results

Two (StoTR03_159 and StoTR04_55) of the nine studied TRs were not detected in any of the seven Senna species, whereas the remaining seven were found in all or some species with patterns that were similar to or contrasted with those of S. tora. Of these studies species, only S. angulata showed significant genome rearrangements and dysploid karyotypes resembling those of S. tora. The genome sizes varied among these species and did not positively correlate with chromosome number. Notably, S. angulata had the fewest chromosomes (2n = 22) but a relatively large genome size.

Discussion

These findings reveal the dynamics of TRs and provide a cytogenetic depiction of chromosomal rearrangements during speciation in Senna. To further elucidate the dynamics of repeat sequences in Senna, future studies must include related species and extensive repeatomic studies, including those on transposable elements.
Would you exchange your soul for immortality?—existential meaning and afterlife beliefs predict mind upload approval
Authors: Laakasuo Michael , Sundvall Jukka , Francis Kathryn , Drosinou Marianna , Hannikainen Ivar , Kunnari Anton , Palomäki Jussi
Published: December 2023

Mind upload, or the digital copying of an individual brain and mind, could theoretically allow one to “live forever.” If such a technology became available, who would be most likely to approve of it or condemn it? Research has shown that fear of death positively predicts the moral approval of hypothetical mind upload technology, while religiosity may have the opposite effect. We build on these findings, drawing also from work on religiosity and existential mattering as predictors of perceived meaning in one’s life. In a cross-sectional study (N = 1,007), we show that existential mattering and afterlife beliefs are negatively associated with moral approval of mind upload technology: people who believe there is a soul or some form of afterlife and who also report a high level of existential mattering, are least likely to morally approve of mind upload technology. Indeed, mind uploading—if it ever becomes feasible—is a form of technology that would fundamentally redraw the existential boundaries of what it means to be human.
Examining acute environmental effects on affective state, expectancy, and intention in sport climbing
Authors: Hösl Benedikt , Niedermeier Martin , Kopp Martin
Published: December 2023

Introduction

Psychological research has shown that, among other variables, affective state, expectancy, and behavioral intention influence whether or not a (physically active) behavior is performed. Environmental effects during physical activity on affective state have been well studied; however, research regarding environmental effects on expectancy or intention is limited. Sport climbing is a form of physical activity that is performed both indoors and outdoors and is therefore considered as suitable to study environmental effects. Therefore, the aim of the present study was to investigate environmental effects during sport climbing on affective state, expectancy, and intention. The nature of the relationship between some of these parameters should also be explored.

Methods

Using a within-subjects design, 48 participants were to climb both once indoors in a climbing gym and once outdoors at a crag. The design included questionnaire-based surveys at multiple time points. Affective state, expectancy, and behavioral intention were measured at different test time points.

Results

Two-factor repeated-measures ANOVAs revealed significant main effects of the factors environment (indoor – outdoor) and time (T1 – T2 – T3) for affective state, indicating more positive affective state during outdoor climbing. No environment*time interactions were found. Furthermore, significantly higher intention (d = 0.32; p = 0.032) was measured after the outdoor condition (M = 32.5, SD = 4.1) compared to the indoor condition (M = 31.2, SD = 4.7). Multiple linear regressions revealed that expectancies (measured before and after the session) significantly predicted post-climbing intention only indoors.

Conclusion

The results at least partly suggest environmental effects on behaviorally relevant variables during climbing. Outdoor climbing might provide more favorable characteristics for physically active behavior compared to indoor climbing. Health psychologists or public health professionals who focus on increasing physical activity for their clients could recommend outdoor climbing over indoor climbing.
Parenting and family self-sufficiency services contribute to impacts of Early Head Start for children and families
Authors: Chazan-Cohen Rachel , Von Ende Adam , Lombardi Caitlin
Published: December 2023

Introduction

There is evidence that two-generation early childhood programs, those that strive to support not only child development, but also optimal parenting and family wellbeing, help to foster resilience for young children and their families in the face of adversity.

Methods

Using data from a large experimental evaluation, the Early Head Start Research and Evaluation Project, this paper explores how parenting and family self-sufficiency services embedded in Early Head Start (EHS), a federally funded, nationally implemented two-generation early childhood program for low-income families lasting from pregnancy and until children are three, contribute to the impacts of the program for both the children and their families.

Results

Parenting support in any modality (home visiting, case management or parent education) contributed to program impacts on important child and family outcomes, but not parent employment. Somewhat surprisingly, family receipt of employment services did not lead to any of the impacts of the program, while education and job training services did. When EHS parents received education or job training services, it led to impacts not only on mother employment, but also on other important family and child outcomes.

Discussion

These findings validate and reinforce the two-generation approach of EHS, specifically supporting the focus on parenting and parent education and job training.
The consumption of psychoactive substances among French physicians: how do they perceive the creation of a dedicated healthcare system?
Authors: Jullian Bénédicte , Deltour Marine , Franchitto Nicolas
Published: December 2023

Introduction

Substance use among physicians can have negative impacts on their health, quality of life, and patient care. While Physician Health Programs (PHPs) have proven effective, many physicians with substance use disorders (SUDs) still face obstacles in seeking help. Our study explores the expectations, attitudes, and experiences of French physicians regarding the implementation of a specialized healthcare system (SHS) for addiction, and their opinions on the factors that could improve the effectiveness of such a service, with a focus on substance use disorders (SUDs).

Methods

We conducted a web-based survey from April 15 to July 15, 2021, which included questions about sociodemographic characteristics, substance use, and attitudes toward a specialized healthcare system (SHS) for physicians with SUDs.

Results

Of the 1,093 respondents (62.5% female), 921 consumed alcohol (84.2%), and 336 (36.4%) were categorized as hazardous drinkers (AUDIT-C ≥ 4 for women and ≥ 5 for men). The mean AUDIT-C score was 3.5 (±1.7 SD), with a range from 1 to 12. Factors associated with hazardous alcohol consumption included coffee consumption [OR 1.53 (1.11–2.12)], psychotropic drug use [OR 1.61 (1.14–2.26)], cannabis use [OR 2.96 (1.58–5.55)], and other drug use [OR 5.25 (1.92–14.35)]. On the other hand, having children was associated with non-hazardous alcohol consumption [OR 0.62 (0.46–0.83)]. Only 27 physicians (2.9%) had consulted a specialist in addiction medicine, while 520 (56.4%) expressed interest in such a consultation. The main barriers to accessing a dedicated consultation were denial (16.3%), physician self-medication (14.3%), fear of judgment (12.8%), and confidentiality concerns (10.2%).

Conclusion

A specialized consultation with trained professionals in a neutral location can improve access to care for healthcare workers and maintain patient confidentiality and anonymity. Prevention and awareness can reduce addiction stigma and help peers in need. The improvement of healthcare workers’ addiction culture and detection of addictive behavior in peers depends on academic addiction medicine.
Multilayer perceptron modeling for social dysfunction prediction based on general health factors in an Iranian women sample
Authors: Bagheri Sajjad , Taridashti Sarvenaz , Farahani Hojjatollah , Watson Peter , Rezvani Elham
Published: December 2023

In the year 2022, this research conducted an in-person study involving 780 single or widowed women, aged between 20 and 70, falling within the bottom three economic deciles and possessing varying levels of education. All participants held educational qualifications below a high school diploma and were beneficiaries of charitable financial support in Khorasan province, Iran. The study aimed to investigate the predictive factors of social dysfunction in this specific demographic. Data collection spanned a 12-month period throughout 2022, with participants completing the GHQ-28 questionnaire during their visits to the charity office. Clinical in-person interviews were also conducted to gather comprehensive data. Data analysis was carried out using IBM SPSS version 27. The research employed a Multilayer Perceptron (MLP) neural network model, considering an extensive set of input factors and covariates. These factors included cognitive functioning, anxiety, depression, age, and education levels. The MLP model exhibited robust performance, achieving high overall accuracy and sensitivity in identifying cases of high social dysfunction. The findings emphasized the significance of cognitive functioning, anxiety, and depression as pivotal predictors of social dysfunction within this specific demographic, while education and age displayed relatively lower importance. The normalized importance scores provided a relative measure of each covariate’s impact on the model’s predictions. These results furnish valuable insights for the development of targeted interventions and evidence-based policies aimed at addressing social dysfunction and promoting societal well-being among economically disadvantaged, single or widowed women. Notably, the research underscores the potential of MLP modeling in social science research and suggests avenues for further research and refinement to enhance the model’s predictive accuracy, particularly for cases of low social dysfunction.
A cost-utility analysis of BRCA1 and BRCA2 testing in high-risk breast cancer patients and family members in Thailand: a cost-effective policy in resource-limited settings
Authors: Lertwilaiwittaya Pongtawat , Tantai Narisa , Maneeon Satanun , Kongbunrak Sophittha , Nonpanya Nongyao , Hurst Anna C. E. , Srinonprasert Varalak , Pithukpakorn Manop
Published: December 2023

Background

Screening for germline pathogenic BRCA1 or BRCA2 variants (gBRCA) in high-risk breast cancer patients is known to be cost-effective in high-income countries. Nationwide adoption of genetics testing in high-risk breast cancer population remains poor. Our study aimed to assess gBRCA health economics data in the middle-income country setting of Thailand.

Methods

Decision tree and Markov model were utilized to assess cost-utility between the testing vs. no-testing groups from a societal and lifetime perspective and lifetime. We interviewed 264 patients with breast/ovarian cancer and their family members to assess relevant costs and quality of life using EQ-5D-5L. One-way sensitivity, probabilistic sensitivity (Monte Carlo simulation), and budget impact analyses were done to estimate the outcome under Thailand's Universal Health Coverage scheme.

Results

The predicted lifetime cost and Quality-adjusted Life Years (QALY) for those with breast cancer were $13,788 and 10.22 in the testing group and $13,702 and 10.07 in the no-testing group. The incremental cost-effectiveness ratio for gBRCA testing in high-risk breast cancer patients was $573/QALY. The lifetime cost for the family members of those with gBRCA was $14,035 (QALY 9.99), while the no-testing family members group was $14,077 (QALY 9.98). Performing gBRCA testing in family members was cost-saving.

Conclusion

Cost-utility analysis demonstrated a cost-effective result of gBRCA testing in high-risk breast cancer patients and cost-saving in familial cascade testing. The result was endorsed in the national health benefits package in 2022. Other middle-income countries may observe the cost-effective/cost-saving aspects in common genetic diseases under their national health schemes.
Ciprofloxacin and pegylated G-CSF combined therapy mitigates brain hemorrhage and mortality induced by ionizing irradiation
Authors: Kiang Juliann G. , Cannon Georgetta , Olson Matthew G. , Zhai Min , Woods Akeylah K. , Xu Feng , Lin Bin , Li Xianghong , Hull Lisa , Jiang Suping , Xiao Mang
Published: December 2023

Introduction

Brain hemorrhage was found between 13 and 16 days after acute whole-body 9.5 Gy 60Co-γ irradiation (IR). This study tested countermeasures mitigating brain hemorrhage and increasing survival from IR. Previously, we found that pegylated G-CSF therapy (PEG) (i.e., Neulasta®, an FDA-approved drug) improved survival post-IR by 20–40%. This study investigated whether Ciprofloxacin (CIP) could enhance PEG-induced survival and whether IR-induced brain hemorrhage could be mitigated by PEG alone or combined with CIP.

Methods

B6D2F1 female mice were exposed to 60Co-γ-radiation. CIP was fed to mice for 21 days. PEG was injected on days 1, 8, and 15. 30-day survival and weight loss were studied in mice treated with vehicles, CIP, PEG, or PEG + CIP. For the early time point study, blood and sternums on days 2, 4, 9, and 15 and brains on day 15 post-IR were collected. Platelet numbers, brain hemorrhage, and histopathology were analyzed. The cerebellum/pons/medulla oblongata were detected with glial fibrillary acidic protein (GFAP), p53, p16, interleukin-18 (IL-18), ICAM1, Claudin 2, ZO-1, and complement protein 3 (C3).

Results

CIP + PEG enhanced survival after IR by 85% vs. the 30% improvement by PEG alone. IR depleted platelets, which was mitigated by PEG or CIP + PEG. Brain hemorrhage, both surface and intracranial, was observed, whereas the sham mice displayed no hemorrhage. CIP or CIP + PEG significantly mitigated brain hemorrhage. IR reduced GFAP levels that were recovered by CIP or CIP + PEG, but not by PEG alone. IR increased IL-18 levels on day 4 only, which was inhibited by CIP alone, PEG alone, or PEG + CIP. IR increased C3 on day 4 and day 15 and that coincided with the occurrence of brain hemorrhage on day 15. IR increased phosphorylated p53 and p53 levels, which was mitigated by CIP, PEG or PEG + CIP. P16, Claudin 2, and ZO-1 were not altered; ICAM1 was increased.

Discussion

CIP + PEG enhanced survival post-IR more than PEG alone. The Concurrence of brain hemorrhage, C3 increases and p53 activation post-IR suggests their involvement in the IR-induced brain impairment. CIP + PEG effectively mitigated the brain lesions, suggesting effectiveness of CIP + PEG therapy for treating the IR-induced brain hemorrhage by recovering GFAP and platelets and reducing C3 and p53.
The associations between social support, self-regulatory fatigue, and health-promoting behaviors among people with type 2 diabetes mellitus: a cross-sectional survey
Authors: Wang Xin , Zhang Fan , Ge Yuanhui , Ding Yiqian , Liu Tao
Published: December 2023

Background

The prevalence of diabetes in China is increasing annually, posing a serious public health challenge. Health-promoting behaviors are crucial for enhancing the quality of life in individuals with type 2 diabetes. However, the relationship between self-regulatory fatigue in type 2 diabetes, social support, and health-promoting behaviors remains unclear.

Objective

This study aimed to explore the interconnections among social support, self-regulatory fatigue, and health-promoting behaviors, as well as to examine the mediating role of self-regulatory fatigue in patients with type 2 diabetes mellitus.

Methods

A cross-sectional design was employed using the Self-Regulatory Fatigue Scale (SRF-S), the Social Support Rating Scale (SSRS), and the Health Promotion Scale for People with Diabetes Mellitus (T2DHPS). These scales assessed social support, self-regulatory fatigue, and health-promoting behaviors in 316 patients with type 2 diabetes mellitus, exploring the relationships among these variables. SPSS and AMOS were used for statistical analysis to investigate the mediating effects.

Results

Social support in type 2 diabetes mellitus positively predicted health-promoting behaviors (β = 0.401, p < 0.001). The regression coefficients of self-regulatory fatigue in patients with type 2 diabetes mellitus social support (β = −0.502, p < 0.001), and health-promoting behaviors (β = −0.331, p < 0.001), both exhibiting significant differences. Self-regulatory fatigue mediated the relationship between social support and health-promoting behaviors in these patients, with a mediation effect of 0.166, consisting of 29.28% of the total effect.

Conclusion

A significant interplay exists among social support, self-regulatory fatigue, and health-promoting behaviors in patients with type 2 diabetes mellitus. The findings suggest that self-regulatory fatigue mediates the relationship between social support and health-promoting behaviors. Healthcare professionals should focus on enhancing patients' social support to mitigate self-regulatory fatigue and improve health behaviors.
Children then, adults now: long-term outcomes—performance at 15, 20, and 25 years of cochlear implant use
Authors: Moura João Elói , Martins Jorge Humberto , Alves Marisa , Oliveira Graça , Ramos Daniela , Alves Helena , Caiado Ricardo , Teixeira António , Silva Luís Filipe , Migueis Jorge
Published: December 2023

Motivation

Severe to profound sensorineural hearing loss interferes with a child's development at the cognitive, linguistic, academic, and social levels. Since the beginning of the pediatric auditory rehabilitation program through cochlear implantation in the Ear, Nose, and Throat (ENT) Service of the Coimbra Hospital and University Center (CHUC), Portugal, its mentors defended the early diagnosis of hearing loss followed by timely intervention, and this was considered the starting point to optimize (re)habilitation through this method. Three decades or so later, recently we conducted this study to evaluate the performance of patients implanted in the initial phase of the cochlear implantation program.

Objectives

The study aimed to evaluate the performance of individuals with severe to profound congenital hearing loss who underwent pediatric cochlear implantation and have used the cochlear implant for at least 25 years, to analyze the beneficial effect of early intervention in improving performance results.

Methods

The study sample is composed of 31 individuals with severe to profound congenital hearing loss and no other comorbidities, divided into two groups (Group 1: age at implantation was under 3 years; Group 2: age at implantation was over 3 years). All 31 subjects were evaluated at 15, 20, and 25 years of cochlear implant (CI) use with a comprehensive set of tests. In addition, data were collected regarding the academic level of each participant. The results of both groups were compared to find out if there is an effect of age at implantation on auditory performance, and if there is an improvement in the performance with CI over time (15, 20, and 25 years of use).

Results

The results show that there is a positive effect, with statistical significance, of early implantation on auditory performance, and telephone use. In both groups, there is an increase in performance over time, but it tends to stabilize after 20 years of CI use.

Discussion and conclusion

The results obtained in this work support the importance of early intervention in patients with severe to profound hearing loss who are cochlear implant users and show that CI is an effective and reliable method in the treatment of these patients, contributing to their improved socio-educational integration, and that the benefits last over time.
What Is El NiñO and How Will It Affect Us?
Authors: Sperschneider Aaron , Foester David , Meurer Pascal , Esters Leonie
Published: December 2023

Imagine a fisherman back in 1726 who relied on catching fish to survive and support his family. When the number of fish went down for any reason, the family would suffer. In 1726, a change in the sea-surface temperature of the Pacific Ocean, which lasted many months, decreased the number of fish. Since then, similar observations have been made in the Pacific Ocean close to the equator. Today, the reasons for the changes in sea temperature are known as the El Niño-Southern Oscillation. It has been studied scientifically since the 1900s. But what is this circulation? In this article, we will explore it in detail and describe its relationship to changes in the environment.
Keeping Your Joints Flexible Throughout Life
Authors: Konrad Andreas , Behm David George
Published: December 2023

People are built to move. To survive, not so long ago, we had to search for food every day as hunters or gatherers. In modern times, however, our way of life has changed drastically. We can buy our food at the supermarket and many people can do their work at a desk. As a result, we move less and sit for several hours every day. This is called a sedentary lifestyle. Sedentary activities can lead to a dramatic decrease in flexibility in the joints. To overcome those challenges, we can do a variety of activities such as performing sports that require the full range of motion of our joints, as well as doing stretch training, foam rolling, or resistance training.
Impact of residue retention and nutrient management on carbon sequestration, soil biological properties, and yield in multi-ratoon sugarcane
Authors: Pradhan Aliza , Wakchaure Goraksha C. , Shid Dhanashri , Minhas Paramjit S. , Biswas Ashis K. , Reddy Kotha Sammi
Published: December 2023

Sequestration of carbon (C) in arable cropping systems is considered one of the potential climate change mitigation strategies. In this context, assessing the potential of sugarcane cropping systems should be a priority, as it leaves substantial amounts of recyclable residues essential for maintaining soil organic carbon (SOC), improving soil health, and strengthening overall resources. We evaluated the impacts of residue retention and nutrient management practices on SOC and its pools, storage, soil biology, and yield in a multi-ratooning sugarcane system. A field experiment was conducted in the split-plot design with residue burning (RB) and residue retention (RR) as the main plot treatments and three nutrient management practices, that is, 25% of the recommended dose of fertilizers (RDF, i.e., 300:150:150 kg of N, P2O5, and K2O kg ha−1, respectively) as basal + 75% through fertigation (N1); 50% of RDF as basal + 50% through fertigation (N2); and 75% of RDF as basal + 25% through fertigation (N3) as subplot treatments in ratoon sugarcane. Soil samples were collected initially and after 6 years of multi-ratooning (one plant and four ratoon crops) from a soil depth of 0–30 cm. The results indicated that RR plots had 21% higher total SOC with 42, 47, 17, and 13% higher very labile, labile, less labile, and non-labile C pools, respectively, than RB plots (P < 0.05). RR also had a higher lability and recalcitrant index than RB. Of the total SOC stock, the contribution of passive pools was higher (72–75%) than active pools. Significantly higher dehydrogenase activity (DHA) (86%), alkaline phosphatase activity (APA) (16%), and ß-glucosidase activity (BGA) (22%) were observed in RR plots as compared to RB plots, whereas for nutrient management practices, it followed the order of N2 > N3> N1. Microbial counts also followed the same trend as that of enzyme activities. Residue retention practices reported higher C sequestration (0.68 Mg C ha−1 yr−1), carbon retention efficiency (37%), and yield (38%) with a potential to reduce GHG emissions by 2.72 Mg CO2 ha−1 yr−1 as compared to traditional practices. Residue retention and 50–75% RDF as basal is recommended for higher soil C retention and soil biology for sustained sugarcane productivity.
Financial Hardship on Food Security in Ageing Populations
Authors: Phulkerd Sirinya , Thapsuwan Sasinee , Chamratrithirong Aphichat , Gray Rossarin Soottipong , Pattaravanich Umaporn , Ungchusak Chantana , Saonuam Pairoj
Published: December 2023

Objective: This study investigated the prevalence of food security, and the association of food security with financial hardship and socio-demographic characteristics among the ageing population in Thailand.

Methods: The study extracted data on 1,197 persons age 60 years or older from a nationally-representative sample survey of Thai households. The food security data were collected using the Food Insecurity Experience Scale (FIES), developed by the Food and Agriculture Organization. Multiple regression analysis was used to investigate the association between financial hardship, socio-demographic characteristics, and food security.

Results: Of the total sample, 71% had food security. The least probability of having food security was observed in the respondents who sometimes and often had income problems (p < 0.001), and felt dissatisfied with their financial situation (p < 0.001). The respondents who were female, at oldest-old age, with lower than primary school education and in the Northeast were less likely to have food security.

Conclusion: These findings suggest the need for government assistance for those who are experiencing financial hardship to help them manage their finances and food security more effectively, taking into account different socio-demographic characteristics.