bluuebunny/crossref_metadata_2025 huggingface dataset:
Near-infrared spectroscopy for early selection of waxy cassava clones via seed analysis
Authors: Sousa Massaine Bandeira e , Filho Juraci Souza Sampaio , de Andrade Luciano Rogerio Braatz , de Oliveira Eder Jorge
Published: January 2023

Cassava (Manihot esculenta Crantz) starch consists of amylopectin and amylose, with its properties determined by the proportion of these two polymers. Waxy starches contain at least 95% amylopectin. In the food industry, waxy starches are advantageous, with pastes that are more stable towards retrogradation, while high-amylose starches are used as resistant starches. This study aimed to associate near-infrared spectrophotometry (NIRS) spectra with the waxy phenotype in cassava seeds and develop an accurate classification model for indirect selection of plants. A total of 1127 F2 seeds were obtained from controlled crosses performed between 77 F1 genotypes (wild-type, Wx_). Seeds were individually identified, and spectral data were obtained via NIRS using a benchtop NIRFlex N-500 and a portable SCiO device spectrometer. Four classification models were assessed for waxy cassava genotype identification: k-nearest neighbor algorithm (KNN), C5.0 decision tree (CDT), parallel random forest (parRF), and eXtreme Gradient Boosting (XGB). Spectral data were divided between a training set (80%) and a testing set (20%). The accuracy, based on NIRFlex N-500 spectral data, ranged from 0.86 (parRF) to 0.92 (XGB). The Kappa index displayed a similar trend as the accuracy, considering the lowest value for the parRF method (0.39) and the highest value for XGB (0.71). For the SCiO device, the accuracy (0.88−0.89) was similar among the four models evaluated. However, the Kappa index was lower than that of the NIRFlex N-500, and this index ranged from 0 (parRF) to 0.16 (KNN and CDT). Therefore, despite the high accuracy these last models are incapable of correctly classifying waxy and non-waxy clones based on the SCiO device spectra. A confusion matrix was performed to demonstrate the classification model results in the testing set. For both NIRS, the models were efficient in classifying non-waxy clones, with values ranging from 96−100%. However, the NIRS differed in the potential to predict waxy genotype class. For the NIRFlex N-500, the percentage ranged from 30% (parRF) to 70% (XGB). In general, the models tended to classify waxy genotypes as non-waxy, mainly SCiO. Therefore, the use of NIRS can perform early selection of cassava seeds with a waxy phenotype.
Determining factors for compensatory movements of the left arm and shoulder in violin playing
Authors: Margulies Oliver , Nübling Matthias , Verheul William , Hildebrandt Wulf , Hildebrandt Horst
Published: January 2023

Introduction

Despite a large number of available ergonomic aids and recommendations regarding instrument positioning, violin players at any proficiency level still display a worrying incidence of task-specific complaints of incompletely understood etiology. Compensatory movement patterns of the left upper extremity form an integral part of violin playing. They are highly variable between players but remain understudied despite their relevance for task-specific health problems.

Methods

This study investigated individual position effects of the instrument and pre-existing biomechanical factors likely determining the degree of typical compensatory movements in the left upper extremity: (1) left elbow/upper arm adduction (“Reference Angle α”, deviation from the vertical axis), (2) shoulder elevation (“Coord x”, in mm), and (3) shoulder protraction (“Coord y”, in mm). In a group of healthy music students (N = 30, 15 m, 15 f, mean age = 22.5, SD = 2.6), “Reference Angle α” was measured by 3D motion capture analysis. “Coord x” and “Coord y” were assessed and ranked by a synchronized 2D HD video monitoring while performing a pre-defined 16-s tune under laboratory conditions. These three primary outcome variables were compared between four typical, standardized violin positions varying by their sideward orientation (“LatAx-CSP”) and/or inclination (“LoAx-HP”) by 30°, as well as the players’ usual playing position. Selected biomechanical hand parameter data were analyzed as co-factors according to Wagner’s Biomechanical Hand Measurement (BHM).

Results

Mean “Reference Angle α” decreased significantly from 24.84 ± 2.67 to 18.61 ± 3.12° (p < 0.001), “Coord x” from 22.54 ± 7.417 to 4.75 ± 3.488 mm (p < 0.001), and “Coord y” from 5.66 ± 3.287 to 1.94 ± 1.901) mm (p < 0.001) when increasing LatAx-CSP and LoAx-HP by 30°. Concerning the biomechanical co-factors, “Reference Angle α”, “Coord y”, but not “Coord x”, were found to be significantly increased overall, with decreasing passive supination range (r = −0.307, p = <0.001 for “Passive Supination 250 g/16Ncm”, and r = −0.194, p = <0.001 for “Coord y”). Compensatory movements were larger during tune sections requiring high positioning of the left hand and when using the small finger.

Discussion

Results may enable to adapt individually suitable instrument positions to minimize strenuous and potentially unhealthy compensation movements of the left upper extremity.
The impact of corporate social responsibility on firm reputation and organizational citizenship behavior: The mediation of organic organizational cultures
Authors: Ali Hussain , Yin Jianhua , Manzoor Faiza , An Mengmeng
Published: January 2023

This study investigates the impact of corporate social responsibility (CSR) on firm reputation and organizational citizenship behavior, along with the mediating inclusion of organic organizational cultures (Clan and Adhocracy) in the medium and large enterprises of Pakistan. To do the path analysis and to investigate the mediating role of organic organization culture, Smart PLS was used. For data collection, the convenience sampling technique was used and responses from 360 questionnaires were the main data source. The results displayed that CSR has a significant and optimistic effect on a firm reputation and employees’ organizational citizenship behavior (OCB). Secondly, the outcomes showed a positive and full mediation effect of organic organizational cultures between CSR and firm reputation but a partial mediation effect of organic organizational cultures between CSR and employees OCB. Further, the results demonstrated a positive and significant influence of employees’ OCB on a firm reputation. The extensive analysis of all factors of the study was autonomously examined to identify the insights that verify how the inclusion of organic organizational cultures can boost the firm reputation and employees’ OCB. Theoretical implications and future research direction are discussed.
Becoming a mother when suffering from a chronic illness
Authors: Riazuelo Hélène
Published: January 2023

Introduction

The critical period of early motherhood when facing serious health problems constitutes a major public health issue. The disease may interfere with, influence, and compound the difficulties experienced over the course of pregnancy and during the parenthood processes. These processes are to be understood as a passage involving so many changes and fraught with difficulties leading to a series of psychological mobilizations. Illness also needs to be considered as a moment of transition, sometimes of severance, marking the lives of the people concerned in a more or less lasting way. Various developments are possible, some of which can be envisaged as leading to more positive outcomes, while others appear as if blocked or doomed to failure.

Clinical data and method

This clinical study is the result of twelve analytically oriented psychotherapeutic follow-ups. The sessions took place weekly over periods ranging from 1 to 6 years. Some of the patients we met had become pregnant while they had a declared but not yet chronic nephropathy. The other patients were already on dialysis and had become mother before starting dialysis. There is also one instance of a pregnant patient on dialysis. In the background, there are also many women who talked about giving up fertility and motherhood. After an in-depth study of each follow-up, a cross-sectional study was conducted to identify the main themes.

Results and discussion

Main considerations on the psychotherapeutic treatment: We regularly observe people who find it impossible to muster the internal resources that would enable them to deal with the trials they undergo in such situations. For the psychologist, there is a need to listen to archaic remnants. Gradually, in the space of psychotherapeutic work, possibilities of “reanimation” of the psyche emerge, an internal space that renews fantasmatic activity as it begins to be able to project into the external space and into the space of the sessions.
CpH methylome analysis in human cortical neurons identifies novel gene pathways and drug targets for opioid use disorder
Authors: Nagamatsu Sheila T. , Rompala Gregory , Hurd Yasmin L. , Núñez-Rios Diana L. , Montalvo-Ortiz Janitza L. ,   Traumatic Stress Brain Research Group
Published: January 2023

Introduction

DNA methylation (DNAm), an epigenetic mechanism, has been associated with opioid use disorder (OUD) in preclinical and human studies. However, most of the studies have focused on DNAm at CpG sites. DNAm at non-CpG sites (mCpHs, where H indicates A, T, or C) has been recently shown to have a role in gene regulation and to be highly abundant in neurons. However, its role in OUD is unknown. This work aims to evaluate mCpHs in the human postmortem orbital frontal cortex (OFC) in the context of OUD.

Methods

A total of 38 Postmortem OFC samples were obtained from the VA Brain Bank (OUD = 12; Control = 26). mCpHs were assessed using reduced representation oxidative bisulfite sequencing in neuronal nuclei. Differential analysis was performed using the “methylkit” R package. Age, ancestry, postmortem interval, PTSD, and smoking status were included as covariates. Significant mCpHs were set at q-value < 0.05. Gene Ontology (GO) and KEGG enrichment analyses were performed for the annotated genes of all differential mCpH loci using String, ShinyGO, and amiGO software. Further, all annotated genes were analyzed using the Drug gene interaction database (DGIdb).

Results

A total of 2,352 differentially methylated genome-wide significant mCpHs were identified in OUD, mapping to 2,081 genes. GO analysis of genes with differential mCpH loci showed enrichment for nervous system development (p-value = 2.32E-19). KEGG enrichment analysis identified axon guidance and glutamatergic synapse (FDR 9E-4–2.1E-2). Drug interaction analysis found 3,420 interactions between the annotated genes and drugs, identifying interactions with 15 opioid-related drugs, including lofexidine and tizanidine, both previously used for the treatment of OUD-related symptoms.

Conclusion

Our findings suggest a role of mCpHs for OUD in cortical neurons and reveal important biological pathways and drug targets associated with the disorder.
Perspective on the potential of tandem-ion mobility/mass spectrometry methods for structural proteomics applications
Authors: Cropley Tyler C. , Chai Mengqi , Liu Fanny C. , Bleiholder Christian
Published: January 2023

Cellular processes are usually carried out collectively by the entirety of all proteins present in a biological cell, i.e., the proteome. Mass spectrometry-based methods have proven particularly successful in identifying and quantifying the constituent proteins of proteomes, including different molecular forms of a protein. Nevertheless, protein sequences alone do not reveal the function or dysfunction of the identified proteins. A straightforward way to assign function or dysfunction to proteins is characterization of their structures and dynamics. However, a method capable to characterize detailed structures of proteins and protein complexes in a large-scale, systematic manner within the context of cellular processes does not yet exist. Here, we discuss the potential of tandem-ion mobility/mass spectrometry (tandem-IM/MS) methods to provide such ability. We highlight the capability of these methods using two case studies on the protein systems ubiquitin and avidin using the tandem-TIMS/MS technology developed in our laboratory and discuss these results in the context of other developments in the broader field of tandem-IM/MS.
Social cohesion and attitudinal changes toward migration: A longitudinal perspective amid the COVID-19 pandemic
Authors: Castillo Juan Carlos , Bonhomme Macarena , Miranda Daniel , Iturra Julio
Published: January 2023

The COVID-19 pandemic has impacted social interactions and coexistence around the globe in dimensions that go far beyond health issues. In the case of the Global South, the pandemic has developed along with growing South-South migratory movements, becoming another key factor that might reinforce social conflict in increasingly multicultural areas as migrants have historically served as “scapegoats” for unexpected crises as a way to control and manage diversity. Chile is one of the main destination countries for migrants from the Latin American and Caribbean region, and COVID-19 outbreaks in migrant housing have intensified discrimination. In such a context, there is a need for understanding how the pandemic has potentially changed the way non-migrants perceive and interact with migrant neighbors. Drawing on the national social cohesion panel survey study ELSOC (2016–2021, N = 2,927) the aim is to analyze the changes in non-migrants' attitudes toward migrants—related to dimensions of social cohesion—over the last years and their relation with individual status and territorial factors. We argue that social cohesion in increasingly multicultural societies is partially threatened in times of crisis. The results indicate that after the pandemic, convivial attitudes toward Latin American migrants decreased. Chileans started perceiving them more negatively, particularly those respondents with lower educational levels and who live in increasingly multicultural neighborhoods with higher rates of migrant residents.
Outcomes after fixation of rib fractures sustained during cardiopulmonary resuscitation: A retrospective single center analysis
Authors: van Veelen Nicole Maria , Buenter Lea , Kremo Valérie , Peek Jesse , Leiser Alfred , Kestenholz Peter , Babst Reto , Paulus Beeres Frank Joseph , Minervini Fabrizio
Published: January 2023

Background

Historically rib fractures have been typically treated non-operatively. Recent studies showed promising results after osteosynthesis of rib fractures in trauma patients with flail segments or multiple rib fractures. However, there is a paucity of data on rib fixation after cardiopulmonary resuscitation (CPR). This study evaluated the outcomes of patients who received rib fixation after CPR.

Methods

Adult patients who received surgical fixation of rib fractures sustained during CPR between 2010 and 2020 were eligible for inclusion in this retrospective study. Outcome measures included complications, quality of life (EQ 5D 5L) and level of dyspnea.

Results

Nineteen patients were included with a mean age of 66.8 years. The mean number of fractured ribs was ten, seven patients additionally had a sternum fracture. Pneumonia occurred in 15 patients (74%), of which 13 were diagnosed preoperatively and 2 post-operatively. Six patients developed a postoperative pneumothorax, none of which required revision surgery. One patient showed persistent flail chest after rib fixation and required additional fixation of a concomitant sternum fracture. One infection of the surgical site of sternal plate occurred, while no further surgery related complications were reported. Mean EQ-5D-5L was 0.908 and the average EQ VAS was 80. One patient reported persisting dyspnea.

Conclusion

To date, this is the largest reported cohort of patients who received rib fixation for fractures sustained during CPR. No complications associated with rib fixation were reported whereas one infection after sternal fixation did occur. Current follow-up demonstrated a good long-term quality of life after fixation, warranting further studies on this topic. Deeper knowledge on this subject would be beneficial for a wide spectrum of physicians.
Analysis of patterns of livestock movements in the Cattle Corridor of Uganda for risk-based surveillance of infectious diseases
Authors: Hasahya Emmanuel , Thakur Krishna , Dione Michel M. , Kerfua Susan D. , Mugezi Israel , Lee Hu Suk
Published: January 2023

Introduction

The knowledge of animal movements is key to formulating strategic animal disease control policies and carrying out targeted surveillance. This study describes the characteristics of district-level cattle, small ruminant, and pig trade networks in the Cattle Corridor of Uganda between 2019 and 2021.

Methodology

The data for the study was extracted from 7,043 animal movement permits (AMPs) obtained from the Ministry of Agriculture, Animal Industry and Fisheries (MAAIF) of Uganda. Most of the data was on cattle (87.2%), followed by small ruminants (11.2%) and pigs (1.6%). Two types of networks representing animal shipments between districts were created for each species based on monthly (n = 30) and seasonal (n = 10) temporal windows. Measures of centrality and cohesiveness were computed for all the temporal windows and our analysis identified the most central districts in the networks.

Results

The median in-degree for monthly networks ranged from 0–3 for cattle, 0–1 for small ruminants and 0–1 for pigs. The highest median out-degrees for cattle, small ruminant and pig monthly networks were observed in Lira, Oyam and Butambala districts, respectively. Unlike the pig networks, the cattle and small ruminant networks were found to be of small-world and free-scale topologies.

Discussion

The cattle and small ruminant trade movement networks were also found to be highly connected, which could facilitate quick spread of infectious animal diseases across these networks. The findings from this study highlighted the significance of characterizing animal movement networks to inform surveillance, early detection, and subsequent control of infectious animal disease outbreaks.
PID-szabályozóval ellátott robothajtás szimulációja Bond-gráf-modell alapján
Authors: Korsoveczki Gyula , Pál Patrik , Husi Géza
Published: January 2023

A tanulmány során egy robothajtáslánc szimulációja valósult meg a 20-sim szimulációs szoftver alkalmazásával. Az említett hajtáslánc egy ideális állandó mágneses egyenáramú motorból, egy sebességcsökkentő fogaskerékpárból, egy nyomatékátvitelt megvalósító torziós tengelyből és egy differenciál-fogaskerék párból áll, amely a mozgás hatásláncának irányát változtatja. A hajtáslánc rendelkezik egy PID-szabályozóval ellátott fordulatszám-szabályozó hurokkal. A tanulmány eredményeként és céljaként alkalmazásra és bizonyításra került a Bond-gráf-alapú modellezés hatékonysága.
Kompresszoros hűtőkörfolyamat szemléltetése és vizsgálata
Authors: Szabó István-Sándor , Pásztor Judit , Farmos Rudolf-László
Published: January 2023

A műszaki oktatás folyamatának fontos része a folyamatok kísérletek és szemléltetőeszközök segítségével történő bemutatása. Ez a közvetlen érzéki tapasztalás az ismeretszerzés egyik forrása. A szemléltetés biztosítja a tananyag mélyebb megértését, így intenzívebbé válik a megismerési folyamat. Dolgozatunkban bemutatunk egy általunk tervezett és megvalósított hűtőgép-szemléltető eszközt, majd egy valós hűtőgépen méréseket végzünk a kompresszoros hűtőkörfolyamat tanulmányozása érdekében. A mérési pontokat a bemutatóeszközön szemléltetjük.
Modeling Firm Search and Innovation Trajectory Using Swarm Intelligence
Authors: Chen Ren-Raw , Miller Cameron D. , Toh Puay Khoon
Published: January 2023

We developed a swarm intelligence-based model to study firm search across innovation topics. Firm search modeling has primarily been “firm-centric,” emphasizing the firm’s own prior performance. Fields interested in firm search behavior—strategic management, organization science, and economics—lack a suitable simulation model to incorporate a more robust set of influences, such as the influence of competitors. We developed a swarm intelligence-based simulation model to fill this gap. To demonstrate how to fit the model to real world data, we applied latent Dirichlet allocation to patent abstracts to derive a topic search space and then provide equations to calibrate the model’s parameters. We are the first to develop a swarm intelligence-based application to study firm search and innovation. The model and data methodology can be extended to address a number of questions related to firm search and competitive dynamics.
Transcriptome Analysis of the Regulatory Mechanism of Exogenous Spermidine in High Temperature Stress Resistance of Tomato Seedlings
Authors: Peng Chen , Shu Sheng , Wang Yu , Du Jing , Shi Lu , Jahan Mohammad Shah , Guo Shirong
Published: January 2023

Previous studies have shown that spermidine (Spd) can improve tolerance to high temperature stress in tomato seedlings. To further understand how Spd regulates the molecular components of high temperature stress signaling pathways, we performed a genome-wide transcriptome analysis in tomato seedlings treated with high temperature and/or exogenous Spd. The results demonstrate that, under high temperature conditions, Spd significantly alleviated the inhibition of plant growth, as well as improving the net photosynthetic rate and pigment contents. The transcriptome analysis revealed thousands of differentially expressed genes (DEGs) in response to high temperature with or without Spd treatment. Half of the genes were induced by high temperature, part of the genes were induced by high temperature with exogenous Spd, and some were induced by the coordinated effect of high temperature and Spd. A GO analysis indicated that genes involved in cellular processes, metabolic processes, and nucleotide binding in the sample were subjected to high temperature. Some DEGs were also involved in plant physiological processes. These results suggest potential genes and molecular pathways were involved in the exogenous Spd-mediated tolerance to high temperature stress in tomato plants. A JA signaling test was designed, which indicated that MYC2 and JAS1 in heat-resistant materials were both increased, through quantitative RT-PCR.
Digital Soil Mapping of Cadmium: Identifying Arable Land for Producing Winter Wheat with Low Concentrations of Cadmium
Authors: Adler Karl , Persson Kristin , Söderström Mats , Eriksson Jan , Pettersson Carl-Göran
Published: January 2023

Intake of cadmium (Cd) via vegetable food poses a possible health risk. Cereals are one of the major sources of Cd, and the Cd concentration in the soil has a great effect on the levels in the grain. The aim of the study was to produce decision support for identification of areas suitable for low-Cd winter wheat production in the form of a detailed digital soil map covering an important agricultural region in southern Sweden. A two-step approach was used: (1) we increased the number of soil Cd observations by combining two sets of soil samples, one with laboratory Cd analyses (304 samples) and one with predicted Cd from a portable x-ray fluorescent (PXRF) sensor (2097 samples); and (2) a digital soil mapping (DSM) model (gradient boosting regression) was calibrated on all 2401 soil samples to create a soil Cd concentration map using a number of covariates, of which airborne gamma ray data was identified as the most important. In the first step, cross-validation of the PXRF model obtained a model efficiency (E) of 0.82 and mean absolute error (MAE) of 0.08 mg kg−1. The DSM model had an E of 0.69 and MAE of 0.11 mg kg−1. The map of predicted soil Cd concentrations were compared against 307 winter wheat (Triticum aestivum L.) grain samples with laboratory-analyzed Cd concentrations. Areas in the map with low soil Cd concentrations had a high frequency of lower grain Cd concentrations. The map thus seemed to have potential for finding areas suitable for production of low-Cd winter wheat; e.g., for baby food.
Silencing CaTPS1 Increases the Sensitivity to Low Temperature and Salt Stresses in Pepper
Authors: Gou Bingdiao , Duan Panpan , Wei Min , Zhao Shufang , Wang Yongfu , Yang Nan , Zhang Gaoyuan , Wei Bingqiang
Published: January 2023

Trehalose, as a non-reductive disaccharide, plays a vital role in plant growth and development and resistance to abiotic stress. Trehalose-6-phosphate synthase (TPS) is a key enzyme in the synthesis mechanism of trehalose and TPS1 genes play a crucial role in the response to abiotic stress in plants. However, it has rarely been reported that CaTPS1 responds to cold and salt stresses in pepper. To verify the function of CaTPS1 in response to cold and salt stresses, CaTPS1 was silenced by virus-induced gene silencing (VIGS). Subsequently, the expressions of CaTPS1, plant morphology and some physiological indexes were analyzed after cold and salt stresses in pepper. The results showed that the expression of CaTPS1 was significantly lower in CaTPS1-silenced (pTRV2-CaTPS1) plant than that in the non-VIGS (CK) and negative control (PTRV2-00) plants. The parameters of response to cold and salt stresses have changed accordingly. The chlorophyll content decreased, while the trehalose content, peroxidase (POD) activity, catalase (CAT) activity and ascorbate peroxidase (APX) activity increased in all treatments. However, these parameters of response to cold and salt stresses were significantly lower in pTRV2-CaTPS1 plant than in CK and PTRV2-00 plants. This study suggested that CaTPS1 was involved in the response to cold and salt stresses in pepper.
Identification of the Genome-Wide Expression Patterns of Non-Coding RNAs Associated with Tanshinones Synthesis Pathway in Salvia miltiorrhiza
Authors: Lin Caicai , Zhou Changhao , Liu Zhongqian , Li Xingfeng , Song Zhenqiao
Published: January 2023

The red root of Salvia miltiorrhiza Bunge, a famous traditional Chinese medicine (TCM), was caused by tanshinone in epidermis cells. In order to study the biological function of ncRNAs in the tanshinone synthesis, the expression patterns of mRNA and ncRNAs were comprehensively analyzed in red (high tanshinone content) and white root (low tanshinone content) tissues derived from the same plant. A total of 731 differentially expressed genes (DEGs) were mainly enriched in primary metabolic pathways such as galactose and nitrogen, and some secondary metabolic pathways such as phenylpropanoid and terpenoids. A total of 70 miRNAs, 48 lncRNAs, and 26 circRNAs were identified as differentially expressed (DE) ones. The enrichment pathway of the targets of DE-lncRNA were mainly in ribosome, carbon metabolism, plant hormone signal transduction, and glycerophospholipid metabolism. The function of the targets genes of 59 miRNAs combined with DE-circRNAs was mainly involved in plant–pathogen interaction, endocytosis, phenylpropanoid biosynthesis, and sesquiterpenoid and triterpenoid biosynthesis pathways. Most genes of the tanshinone synthesis pathway had a higher expression. Some ncRNAs were predicted to regulate several key enzyme genes of the tanshinone synthesis pathway, such as SmDXS2, SmGGPPS1, SmKSL. Furthermore, most target genes were related to the resistance of pathogens. The present study exhibited the tissue-specific expression patterns of ncRNAs, which would provide a basis for further research into the regulation mechanism of ncRNAs in the tanshinone synthesis process.
Identification of Eco-Climatic Factors Driving Yields and Genotype by Environment Interactions for Yield in Early Maturity Soybean Using Crop Simulation
Authors: Elmerich Chloé , Boulch Guénolé , Faucon Michel-Pierre , Lakhal Lyes , Lange Bastien
Published: January 2023

Deploying crops in regions bordering their initial distribution area requires adapting existing cultivars to particular environmental constraints. In this study, we revealed the main Eco-climatic Factors (EFs)—climatic factors recorded over specific phenological periods—impacting both yields and Genotype by Environment Interactions (GEI) for yield in early maturity soybeans (Glycine max (L.) Merrill) under high latitudes. A multi-year (2017–2021) and multi-environment (n = 112) database was built based on the official post-inscription French soybean trial network “SOJA Terres Inovia-GEVES-Partenaires”. Yields of 57 cultivars covering MG00 and MG000 maturity groups were considered. For each environment, 126 EFs were calculated using a Crop Growth Model (CGM) based on observed weather data and simulated developmental stages. Partial Least Square (PLS) regression analyses using the Variable Importance in Projection (VIP) score were used to sort out the most relevant EFs for their impact on yield levels on the one side and on GEI for yield on the other side. Our results confirmed that yield levels for both maturity groups were greatly influenced by climatic factors from the seed filling phenophases, mainly End of Pod to Physiological Maturity. The cumulative potential evapotranspiration during the End of Pod to Physiological Maturity period was the main EF affecting yield levels positively for both maturity groups (VIP = 2.86; R2 = 0.64). Interestingly, EFs explaining yield levels strongly differed from those explaining GEI, in terms of both climatic factors and phenophases. GEI were mostly influenced by climatic factors from First Flower to End of Pod; these factors were maximum temperatures and solar radiation intensity. Cold stress from Sowing to First Seed also appeared to be a critical driver for GEI in MG00 soybeans. The contrasted responses of several cultivars to the main GEI-drivers highlighted a potential genetic variability that could be exploited in early maturity soybean breeding. This study revealed the complexity of GEI ecophysiology, and our results should help breeding strategies to deliver germplasm that outperforms the existing genetic material for expanding the crop to northern European regions.
Hydrogen Peroxide Ameliorates the Adversities of Drought Stress during Germination and Seedling Growth in Sorghum (Sorghum bicolor L.)
Authors: Song Ki Eun , Hwang He Rin , Hong e Sun Hee , Konvalina Petr , Jun Woo Jin , Jung Jin Woong , Shim Sangin
Published: January 2023

Proper seed germination is important for seedling establishment and growth in fields under drought caused by climate change. In the present study, the beneficial effects of hydrogen peroxide on seed germination were investigated by proteome analysis. Sorghum seeds were subjected to drought stress adjusted to the various water potentials of 0, −0.2, and −0.5 MPa using polyethylene glycol (PEG) 6000 and treated with hydrogen peroxide at different concentrations (0, 10, 20, 50, and 100 mM). Germination percentage and seedling growth were determined at 6 days after imbibition, and proteins from embryos were analyzed. As a result of the study, it was found that the reduced germination percentage and seedling growth under drought stress were alleviated by hydrogen peroxide treatment. Proteins induced in hydrogen peroxide-treated embryos included glycolysis-related enzymes (25%) and stress-defense proteins (30%). Among the glycolysis-related enzymes, fructokinase-1 was higher only under drought and 0 mM H2O2 treatment, and phosphoglycerate kinase was higher than control under drought and 100 mM H2O2 treatment. Hydrogen peroxide treatment also increased the expression of antioxidant enzymes related to stress defense. The results that hydrogen peroxide treatment increases germination rate and seedling growth by increasing energy production and defense activity suggest a practical application of H2O2 at 100 mM for reducing the drought stress in sorghum.
Genome-Wide Identification, Characterization, and Expression Analysis of the U-Box Gene Family in Punica granatum L.
Authors: Chen Lide , Ge Dapeng , Ren Yuan , Wang Yuying , Yan Ming , Zhao Xueqing , Yuan Zhaohe
Published: January 2023

The ubiquitination pathway is essential for several developmental phases in plants, and the U-box protein family of ubiquitin ligases plays an important role in this process. However, little is known about pomegranate’s PUB genes. In this investigation, the pomegranate U-box gene family was identified using whole-genome sequencing data. We identified a total of 56 members of the pomegranate U-box family based on the U-box domain, and the PgPUBs were classified into four groups. Chromosomal localization, phylogenetic analysis, motif distribution, gene duplications, cis-acting elements, and expression profiling were also investigated. The PgPUB genes were unevenly distributed among the eight pomegranate chromosomes, and collinear duplicated genes were identified between the Arabidopsis thaliana genome and the Punica granatum genome. Furthermore, the gene expression analysis revealed that expression of U-box genes in pomegranate was induced by abiotic stressors. Collectively, our findings provide insight into the U-box gene family and will assist in understanding the functional divergence of U-box genes in Punica granatum L.
Full-Fat Black Soldier Fly Larvae Meal in Diet for Tambaqui, Colossoma macropomum: Digestibility, Growth Performance and Economic Analysis of Feeds
Authors: Monteiro dos Santos Driely Kathriny , Rodrigues de Freitas Odair , Oishi Cesar Augusto , Leão da Fonseca Flávio Augusto , Parisi Giuliana , Uribe Gonçalves Ligia
Published: January 2023

Black soldier fly (BSF, Hermetia illucens) larvae is a prominent ingredient in aquafeeds due to its high protein and energy contents. This study evaluated the digestibility of full-fat BSF larvae meal (FF-BSFL) and its inclusion in diets for tambaqui, Colossoma macropomum. The apparent digestibility coefficient of FF-BSFL for protein and energy was around of 88%, corresponding to 33.55% and 21.61 MJ kg−1 of digestible protein and energy, respectively. For the feeding trial, tambaqui juveniles (53.23 ± 1.07 g) were distributed in a completely randomized experimental design (n = 4; 150 L tanks; 10 fish per tank). Fish were fed diets including 0%, 5.25%, 10.50%, and 15.75% FF-BSFL to apparent satiation for 60 days. Fish fed 10.50% FF-BSFL dietary inclusion showed higher weight gain, feed intake, final biomass, and relative growth rate. The 10.50% FF-BSFL diet presented the highest index of economic profitability. Weight gain fitted a third-degree equation and the optimum FF-BSFL inclusion level was estimated at 11.6%. However, FF-BSFL dietary inclusion up to 15.75% did not impair growth fish performance. FF-BSFL seems to be a promising source of protein and energy for omnivorous fish aquafeed.
Comparative Analyses Reveal the Genetic Mechanism of Ambergris Production in the Sperm Whale Based on the Chromosome-Level Genome
Authors: Zhou Chuang , Peng Kexin , Liu Yi , Zhang Rusong , Zheng Xiaofeng , Yue Bisong , Du Chao , Wu Yongjie
Published: January 2023

Sperm whales are a marine mammal famous for the aromatic substance, the ambergris, produced from its colon. Little is known about the biological processes of ambergris production, and this study aims to investigate the genetic mechanism of ambergris production in the sperm whale based on its chromosome-level genome. Comparative genomics analyses found 1207 expanded gene families and 321 positive selected genes (PSGs) in the sperm whale, and functional enrichment analyses suggested revelatory pathways and terms related to the metabolism of steroids, terpenoids, and aldosterone, as well as microbiota interaction and immune network in the intestine. Furthermore, two sperm-whale-specific missense mutations (Tyr393His and Leu567Val) were detected in the PSG LIPE, which has been reported to play vital roles in lipid and cholesterol metabolism. In total, 46 CYP genes and 22 HSD genes were annotated, and then mapped to sperm whale chromosomes. Furthermore, phylogenetic analysis of CYP genes in six mammals found that CYP2E1, CYP51A and CYP8 subfamilies exhibited relative expansion in the sperm whale. Our results could help understand the genetic mechanism of ambergris production, and further reveal the convergent evolution pattern among animals that produce similar odorants.
The Effects of Dietary Spirulina platensisis on Physiological Responses of Broiler Chickens Exposed to Endotoxin Stress
Authors: Alaqil Abdulaziz A. , Abbas Ahmed O.
Published: January 2023

This study was proposed to highlight the impact of dietary Spirulina platensis (SP) supplementation in alleviating the deterioration effect of Escherichia coli (EC) on the growth performance, redox biomarkers, immune reaction, and hindgut microbial counts and acidosis in broiler chickens. Four hundred Cobb500, one-day-old, broiler chickens were deposited in battery cages (10 chicks per cage). The chicks were distributed into totally randomized 2 × 2 factorial treatments (10 replicate cages per treatment) from the day 22 to the day 42 of age. Birds of two of the groups were fed on a basal diet without SP supplementation (-SP groups), while birds of the other two groups were fed on a basal diet supplemented with 10 g/kg SP (+SP groups). At day 36th of age, birds in one of the -SP and +SP groups were challenged by an intraperitoneal (i.p.) injection with 107 CFU/bird EC (O157:H7 strain) in 0.5 mL sterilized saline (+EC groups), whereas the other non-challenged groups were i.p. injected with 0.5 mL saline only (-EC groups). The current study results indicated that the boilers challenged with EC had a significant (p < 0.05) lower performance, poor antioxidant activity, immunosuppression, and higher numbers of pathogenic bacteria in the intestine when compared with the non-challenged birds. Dietary SP inclusion enhanced (p < 0.05) broiler growth, antioxidant activity, immune response, and intestinal beneficial bacteria and acidosis. Moreover, SP alleviated the reduction in all these parameters after exposure to EC infection. Therefore, diets containing 10 g/kg SP could be used as a promising approach to maximize broilers’ production and support their health, particularly when challenged with EC infection.
Effects of Different Dietary Protein Level on Growth Performance, Rumen Fermentation Characteristics and Plasma Metabolomics Profile of Growing Yak in the Cold Season
Authors: Zhu Yanbin , Sun Guangming , Dunzhu Luosang , Li Xin , Zhaxi Luosang , Zhaxi Suolang , Suolang  , Ciyang  , Yangji Cidan , Wangdui Basang , Pan Feng , Peng Quanhui
Published: January 2023

This experiment was aimed to compare the effects of two diets with different protein content on the growth performance, immune indexes, rumen fermentation characteristics and plasma metabolomics of growing yak in the cold season. A total of 24, 2-year-old healthy yaks with similar body weight (142.9 ± 3.56 kg) were randomly allocated to two isoenergetic diets with different protein content (10 vs 14%) according to a non-paired experimental design, and the protein of the diets was increased by increasing soybean meal, rapeseed meal and cottonseed meal. The growth performance experiment lasted 56 days. Four days before the end of the growth experiment, the digestion trial was conducted, and the rumen fluid and plasma was collected for measurement. The results showed that the average daily feed intake (p < 0.001) and average daily gain (p = 0.006) of yak fed a high-protein diet was significantly greater, while the feed conversion ratio was lower (p = 0.021) than that of yaks fed a low-protein diet. Plasma aspartate aminotransferase (p = 0.002), alanine aminotransferase (p < 0.001), malondialdehyde (p = 0.001), tumor necrosis factor-α (p = 0.032) and interferon-γ (p = 0.017) of the high-protein group were significantly lesser, whereas superoxide dismutase (p = 0.004) and interleukin-2 (p = 0.007) was significantly greater than that of the low-protein group. The rumen microbial crude protein (p < 0.047) and crude protein digestibility (p = 0.015) of yak fed a high-protein diet was significantly greater than that of the low-protein group. The metabolomics results showed that yaks fed a high-protein diet were elevated in protein digestion and absorption, arginine and proline metabolism, tryptophan metabolism, purine metabolism, butanoate metabolism, taste transduction, pyrimidine metabolism, pantothenate and CoA biosynthesis, glutathione metabolism and renin secretion pathways. It is concluded that a high-protein diet in the cold season can promote rumen microbial crude protein synthesis, enhance antioxidant and immune function and promote growth performance of yaks.
Pregabalin Alleviates Anxiety and Fear in Cats during Transportation and Veterinary Visits—A Clinical Field Study
Authors: Lamminen Terttu , Korpivaara Mira , Aspegrén John , Palestrini Clara , Overall Karen L.
Published: January 2023

Cats frequently suffer from anxiety related to travel and veterinary visits. One sequela is avoidance of veterinary visits and lack of adequate veterinary care. The objective of this study was to test clinical efficacy and safety of a novel formulation of a pregabalin 50 mg/mL oral solution for alleviation of anxiety and fear in cats during transport and veterinary visits. A total of 209 client-owned cats were given either a flavored pregabalin oral solution at the dosage of 5 mg/kg (n = 108) or an identical placebo (n = 101) approximately 90 min before placing them into the carrier and transporting them in a car for at least 20 min to a veterinary clinic. The treatment effect using a 5-point numerical rating scale was evaluated during transportation by the owner and during clinical examination by the veterinarian, both blinded to the treatment. In addition, to verify the owner assessment, an external expert blinded to the treatment and owner assessment evaluated the transportation video recordings using the same rating scale as the owner. Pregabalin 5 mg/kg statistically significantly decreased both travel- (p < 0.01) and veterinary-visit- (p < 0.01) related anxiety compared to the placebo. The external expert’s evaluation was in agreement with the owners’ assessment confirming the treatment effect during transportation (p < 0.01). Treatment was well tolerated with only a few cats showing transient slight incoordination and tiredness. The flavored oral solution formulation with a small dosing volume of 0.1 mL/kg was found by the owners to be user-friendly and was well-accepted by the cats. This study demonstrated that a single oral dosage of the novel pregabalin oral solution alleviates anxiety and fear related to transportation and veterinary visits in cats, thus providing practical aid for both owners and veterinarians to enable cat-friendly handling and improving the welfare of cats in situations they often perceive as very stressful.
Early Supplementation with Starter Can Improve Production Performance of Lambs but this Growth Advantage Disappears after 154 Days of Age
Authors: Xu Jianfeng , Li Fadi , Zhang Zhendong , Zheng Chen , Shen Zhenfeng , Ma Zhiyuan , Wang Jing , Zhang Fan , Wang Jiaqi , Ran Hang , Yun Ying , Liu Ting
Published: January 2023

The aim of this experiment was to study the sustained effects of early supplementation with a starter on the performance of lambs during the pre-weaning, post-weaning, and fattening periods. Sixty male Hu lambs (3.59 ± 0.05 kg) were randomly assigned to two (30 lambs per group) treatments. The lambs were fed milk replacer from three days of age. The early supplementation (ES) group was supplemented with a starter ration at seven days of age, the control (CON) group was supplemented at 21 days of age, and lambs in both groups were weaned from milk replacer at 28 days of age. Eight lambs from each group were randomly slaughtered at 98 days of age, and the remaining lambs were fed the same nutrient level of a fattening ration until slaughter at 200 days of age. The results showed that early supplementation with starter significantly improved average daily feed intake (ADFI) and average daily weight gain (ADG) in the pre-weaning period (7–28 days of age), and ADFI and slaughter performance (live weight before slaughter, carcass weight and dressing percentage) in the post-weaning period (29–98 days of age, p < 0.05). In addition, early supplementation with the starter had no significant effect (p > 0.05) on ADFI, ADG, and slaughter performance, but significantly decreased (p < 0.05) the feed-to-gain ratio (F/G) of lambs during the fattening period (99–200 days of age). In addition, early supplementation with starter increased the ratio of rumen and reticulum weight to total stomach weight in lambs (p < 0.05). In conclusion, early supplementation with starter can reduce feed costs and improve the performance of lambs, while the growth advantage produced by early supplementation had initially disappeared by 154 days of age.
Bone Healing Process of a Multiple Humeral Fracture in a Caretta caretta: Clinical, Surgical, Radiographic and Histomorphometric Assessments
Authors: Valastro Carmela , Di Comite Mariasevera , Paci Serena , Franchini Delia , Ciccarelli Stefano , Di Bello Antonio
Published: January 2023

This study describes the surgical treatment of multiple humeral fractures in a Caretta caretta sea turtle referred by the ‘Centro Faunistico del Parco Regionale Bosco e Paludi di Rauccio’, in the area surrounding the city of Lecce, in southern Italy. Radiographs showed an evident detachment of the distal humeral epiphysis, compatible with a type II Salter-Harris fracture, as well as a transverse fracture of the diaphysis. After the surgical fracture reduction, radiographic follow-up was performed at 2, 4, 12, 16, and 24 months, showing a progressive healing and the formation of poorly mineralized callus tissue. Unfortunately, three months after his release at sea, the turtle was caught dead at a depth of 40 m. Histological and histomorphometric examinations of the surgically treated humerus were carried out on the corpse to collect further information about the bone tissue repair mechanisms in these animals.
The Two-Component System CpxRA Affects Antibiotic Susceptibility and Biofilm Formation in Avian Pathogenic Escherichia coli
Authors: Ma Kai , Wang Hui , Lv Zhenfei , Hu Yutong , Wang Hongli , Shu Fang , Zhu Chengfeng , Xue Ting
Published: January 2023

Avian pathogenic Escherichia coli (APEC) is one of the common extraintestinal infectious disease pathogens in chickens, geese, and other birds. It can cause a variety of infections, and even the death of poultry, causing enormous economic losses. However, the misuse and abuse of antibiotics in the poultry industry have led to the development of drug resistance in the gut microbes, posing a challenge for the treatment of APEC infections. It has been reported that the CpxRA two-component system has an effect on bacterial drug resistance, but the specific regulatory mechanism remains unclear. In this study, the regulatory mechanism of CpxRA on APEC biofilm formation and EmrKY efflux pump was investigated. The cpxRA knockout strain of E. coli APEC40 was constructed, and the molecular regulatory mechanism of CpxR on biofilms and efflux pump-coding genes were identified by biofilm formation assays, drug susceptibility test, real-time reverse transcription quantitative PCR, and electrophoretic mobility shift assay (EMSA). The results indicated that CpxR can directly bind to the promoter region of emrKY and negatively regulate the sensitivity of bacteria to ofloxacin and erythromycin. These results confirm the important regulatory role of the CpxRA two-component system under antibiotic stress in APEC.