bluuebunny/crossref_metadata_2025 huggingface dataset:
On the Person-Place Interaction and Its Relationship with the Responses/Outcomes of Listeners of Urban Soundscape (Compared Cases of Lisbon and Bogotá): Contextual and Semiotic Aspects
Authors: Hermida Luis , Pavón Ignacio , Lobo Soares Antonio Carlos , Bento-Coelho J. Luis
Published: February 2019

Design, planning, and management of the urban soundscape require various interacting fields of knowledge given the fact that it is the human person that experiences and provides meaning to the urban places and their acoustic environments. The process of environmental perception involves contextual information that conditions people’s responses and outcomes through the relationship between the variables Person, Activity, and Place. This research focuses on the interaction between Person and Place and its impact on responses and outcomes from listeners with different geographical origin and background. Laboratory studies were conducted in the cities of Lisbon (Portugal) and Bogotá (Colombia), where local listeners were introduced to known and unknown acoustic environments. Sound data recorded in the two cities allowed comparison of responses and outcomes of the listeners according to the Person-Place Interaction, leading to different meanings depending on the contextual variables. The results clearly show a relationship between site, acoustic environment, soundscape, Person-Place Interaction, and meaning of the place. This information can be useful for urban technicians and designers dealing with planning and management of urban soundscapes.
Hierarchy of Factors Affecting the Condition and Development of Sports and Recreation Infrastructure—Impact on the Recreational Activity and Health of the Residents of a City (Poznan Case Study)
Authors: Kruszyńska Ewa , Poczta Joanna
Published: February 2019

The aim of the article is to identify which groups of factors (economic, social, and spatial) significantly determine the condition and development of the sports and recreation infrastructure of the city of Poznan and shape the needs and expectations of its residents. Standardized interviews among 39 service providers and 1159 service recipients made it possible to collect primary data on the presentation of the pace and directions of changes taking place in the sports and recreational facilities of the city, paying special attention to identifying and prioritizing factors determining this development. In order to establish the hierarchy of factors analyzed in the paper and operating within the same research problem for both groups of respondents (service recipients and service providers), the Anderson-Darling test was used. The test results were referenced to the already existing “Sportowy Poznan” (“Sports Poznan”) program. The assessment made by service recipients shows that the factor having the strongest limiting effect on the use of sports and recreation services is the economic factor. In the assessment made by the respondents using their services, the efforts of city authorities to make Poznan sports clubs operate in a professional manner gained the lowest score. The results of the Anderson–Darling test show that the social factor of preparing infrastructure for the residents of the city of Poznan was the most important for the respondents, obtaining a test value of 0.886.
Studying Social Uses of 3D Geovisualizations: Lessons Learned from Action-Research Projects in the Field of Flood Mitigation Planning
Authors: Jacquinod Florence , Bonaccorsi Julia
Published: February 2019

Risk management seeks more and more the mobilization of all citizens, including elected representatives and inhabitants. Three-dimensional (3D) geovisualizations have been used between 2009 and 2017 in order to associate citizens to flood mitigation policies along the river Rhône. We focused our studies on the effects 3D geovisualizations can have on the communication and understanding of information and their ability to foster exchanges between heterogeneous actors as well as participation of the grand public to planning processes. Facing both discrepancies in scientific studies of the uses of 3D geovisualizations and a lack of validated theoretical elements, we resorted to an exploratory method based on grounded theory and ethnographic observation in order to produce empirical knowledge on the uses of 3D geovisualizations in collective settings, including heterogeneous actors (risk managers, elected representatives, citizens). Observation showed that 3D geovisualizations can be useful for the dissemination of information about flood risk. Many observed effects were not anticipated during the production of 3D geovisualizations. Qualitative analysis of empirical data through actor–network theory and from a communication studies perspective shed light on some factors influencing the roles of 3D geovisualizations and help put into perspective existing and sometimes contradictory scientific works on 3D geovisualizations’ uses.
Scenario-Based Risk Assessment of Earthquake Disaster Using Slope Displacement, PGA, and Population Density in the Guyuan Region, China
Authors: Zhuang Jianqi , Peng Jianbing , Zhu Xinghua , Huang Weiliang
Published: February 2019

Mega-earthquakes that occur in mountainous areas of densely populated cities are particularly catastrophic, triggering large landslides, destroying more buildings, and usually resulting in significant death tolls. In this paper, earthquake scenarios in the Guyuan Region of China are used as an example to study earthquake disaster risk assessment and a method of assessment is proposed that uses the peak ground acceleration (PGA), landslides triggered by the earthquake, and the effects on the population. The method is used to develop scenarios for earthquake disaster risk assessment along the Haiyuan and Liupanshan Faults for earthquake magnitudes of Ms 7.0, 7.5, 8.0, and 8.5 triggered by one of the two faults. The quantitative earthquake disaster risk maps in the study area were developed by integrating the values of the at-risk elements for the earthquake factor, population, and landslide hazard. According to the model results, the high-hazard zone was mainly located in the severely affected areas along the faults and on the western side of the faults. These results can be useful for emergency preparation planning, response plans, and resource assessment.
Molecular Implications of Natriuretic Peptides in the Protection from Hypertension and Target Organ Damage Development
Authors: Rubattu Speranza , Forte Maurizio , Marchitti Simona , Volpe Massimo
Published: February 2019

The pathogenesis of hypertension, as a multifactorial trait, is complex. High blood pressure levels, in turn, concur with the development of cardiovascular damage. Abnormalities of several neurohormonal mechanisms controlling blood pressure homeostasis and cardiovascular remodeling can contribute to these pathological conditions. The natriuretic peptide (NP) family (including ANP (atrial natriuretic peptide), BNP (brain natriuretic peptide), and CNP (C-type natriuretic peptide)), the NP receptors (NPRA, NPRB, and NPRC), and the related protease convertases (furin, corin, and PCSK6) constitute the NP system and represent relevant protective mechanisms toward the development of hypertension and associated conditions, such as atherosclerosis, stroke, myocardial infarction, heart failure, and renal injury. Initially, several experimental studies performed in different animal models demonstrated a key role of the NP system in the development of hypertension. Importantly, these studies provided relevant insights for a better comprehension of the pathogenesis of hypertension and related cardiovascular phenotypes in humans. Thus, investigation of the role of NPs in hypertension offers an excellent example in translational medicine. In this review article, we will summarize the most compelling evidence regarding the molecular mechanisms underlying the physiological and pathological impact of NPs on blood pressure regulation and on hypertension development. We will also discuss the protective effect of NPs toward the increased susceptibility to hypertensive target organ damage.
Garcinol Sensitizes NSCLC Cells to Standard Therapies by Regulating EMT-Modulating miRNAs
Authors: Farhan Mohd , Malik Arshi , Ullah Mohammad Fahad , Afaq Sarah , Faisal Mohd , Farooqi Ammad Ahmad , Biersack Bernhard , Schobert Rainer , Ahmad Aamir
Published: February 2019

Garcinol, a dietary factor obtained from Garcinia indica, modulates several key cellular signaling pathways as well as the expression of miRNAs. Acquired resistance to standard therapies, such as erlotinib and cisplatin, is a hallmark of non-small cell lung cancer (NSCLC) cells that often involves miRNA-regulated epithelial-to-mesenchymal transition (EMT). We used A549 cells that were exposed to transforming growth factor beta 1 (TGF-β1), resulting in A549M cells with mesenchymal and drug resistant phenotype, and report that garcinol sensitized resistant cells with mesenchymal phenotype to erlotinib as well as cisplatin with significant decrease in their IC50 values. It also potentiated the apoptosis-inducing activity of erlotinib in A549M and the endogenously mesenchymal H1299 NSCLC cells. Further, garcinol significantly upregulated several key EMT-regulating miRNAs, such as miR-200b, miR-205, miR-218, and let-7c. Antagonizing miRNAs, through anti-miRNA transfections, attenuated the EMT-modulating activity of garcinol, as determined by mRNA expression of EMT markers, E-cadherin, vimentin, and Zinc Finger E-Box Binding Homeobox 1 (ZEB1). This further led to repression of erlotinib as well as cisplatin sensitization, thus establishing the mechanistic role of miRNAs, particularly miR-200c and let-7c, in garcinol-mediated reversal of EMT and the resulting sensitization of NSCLC cells to standard therapies.
Unravelling the Complexity of Plant Defense Induced by a Simultaneous and Sequential Mite and Aphid Infestation
Authors: Kiełkiewicz Małgorzata , Barczak-Brzyżek Anna , Karpińska Barbara , Filipecki Marcin
Published: February 2019

In natural and agricultural conditions, plants are attacked by a community of herbivores, including aphids and mites. The green peach aphid and the two-spotted spider mite, both economically important pests, may share the same plant. Therefore, an important question arises as to how plants integrate signals induced by dual herbivore attack into the optimal defensive response. We showed that regardless of which attacker was first, 24 h of infestation allowed for efficient priming of the Arabidopsis defense, which decreased the reproductive performance of one of the subsequent herbivores. The expression analysis of several defense-related genes demonstrated that the individual impact of mite and aphid feeding spread systematically, engaging the salicylic acid (SA) and jasmonic acid (JA) signaling pathways. Interestingly, aphids feeding on the systemic leaf of the plant simultaneously attacked by mites, efficiently reduced the magnitude of the SA and JA activation, whereas mites feeding remotely increased the aphid-induced SA marker gene expression, while the JA-dependent response was completely abolished. We also indicated that the weaker performance of mites and aphids in double infestation essays might be attributed to aliphatic glucosinolates. Our report is the first to provide molecular data on signaling cross-talk when representatives of two distinct taxonomical classes within the phylum Arthropoda co-infest the same plant.
DNA Repair Gene ZmRAD51A Improves Rice and Arabidopsis Resistance to Disease
Authors: Liu Fang , Xu Yunjian , Zhou Lingyan , Ali Asif , Jiang Haiyang , Zhu Suwen , Li Xiaoyu
Published: February 2019

RAD51 (DNA repair gene) family genes play ubiquitous roles in immune response among species from plants to mammals. In this study, we cloned the ZmRAD51A gene (a member of RAD51) in maize and generated ZmRAD51A overexpression (ZmRAD51A-OE) in rice, tobacco, and Arabidopsis. The expression level of ZmRAD51A was remarkably induced by salicylic acid (SA) application in maize, and the transient overexpression of ZmRAD51A in tobacco induced a hypersensitive response. The disease resistance was significantly enhanced in ZmRAD51A- OE (overexpressing) plants, triggering an increased expression of defense-related genes. High-performance liquid chromatography (HPLC) analysis showed that, compared to control lines, ZmRAD51A-OE in rice plants resulted in higher SA levels, and conferred rice plants resistance to Magnaporthe oryzae. Moreover, the ZmRAD51A-OE Arabidopsis plants displayed increased resistance to Pseudomonas syringae pv. tomato DC3000 when compared to wild types. Together, our results provide the evidence that, for the first time, the maize DNA repair gene ZmRAD51A plays an important role in in disease resistance.
Biomarker Analysis of Orally Dosed, Dual Active, Matrix Metalloproteinase (MMP)-2 and MMP-9 Inhibitor, AQU-118, in the Spinal Nerve Ligation (SNL) Rat Model of Neuropathic Pain
Authors: Kwan Mei Yee , Choo Anthony , Hanania Taleen , Ghavami Afshin , Beltran Jose , Shea John , Barboza Amidi , Hu Andrew , Fowler Marcie , Neelagiri Venugopal Rao , Sucholeiki Irving
Published: February 2019

There is an unmet medical need for the development of non-addicting pain therapeutics with enhanced efficacy and tolerability. The current study examined the effects of AQU-118, an orally active inhibitor of metalloproteinase-2 (MMP-2) and MMP-9, in the spinal nerve ligation (SNL) rat model of neuropathic pain. Mechanical allodynia and the levels of various biomarkers were examined within the dorsal root ganglion (DRG) before and after oral dosing with AQU-118. The rats that received the SNL surgery exhibited significant mechanical allodynia as compared to sham controls. Animals received either vehicle, positive control (gabapentin), or AQU-118. After SNL surgery, the dorsal root ganglion (DRG) of those rats dosed with vehicle had elevated messenger RNA (mRNA) expression levels for MMP-2, IL1-β & IL-6 and elevated protein levels for caspase-3 while exhibiting decreased protein levels for myelin basic protein (MBP) & active IL-β as compared to sham controls. Rats orally dosed with AQU-118 exhibited significantly reduced mechanical allodynia and decreased levels of caspase-3 in the DRG as compared to vehicle controls. Results demonstrate that oral dosing with the dual active, MMP-2/-9 inhibitor, AQU-118, attenuated mechanical allodynia while at the same time significantly reduced the levels of caspase-3 in the DRG.
Identification of Key Aroma Compounds in Type I Sourdough-Based Chinese Steamed Bread: Application of Untargeted Metabolomics Analysisp
Authors: Yan Bowen , Sadiq Faizan A. , Cai Yijie , Fan Daming , Zhang Hao , Zhao Jianxin , Chen Wei
Published: February 2019

Untargeted metabolomics is a valuable tool to analyze metabolite profiles or aroma fingerprints of different food products. However, less attention has been paid to determining the aroma characteristics of Chinese steamed breads (CSBs) by using this approach. The aim of this work was to evaluate the key aroma compounds and their potential generation pathway in Chinese steamed bread produced with type I sourdough by a metabolomics approach. Based on the aroma characteristics analysis, CSBs produced with type I sourdough and baker’s yeast were clearly distinguishable by principal component analysis (PCA) scores plot. A total of 13 compounds in sourdough-based steamed breads were given the status of discriminant markers through the untargeted metabolomics analysis. According to the odor activity values (OAVs) of discriminant aroma markers, ethyl acetate (fruity), ethyl lactate (caramel-like), hexyl acetate (fruity), (E)-2-nonenal (fatty) and 2-pentylfuran (fruity) were validated as the key volatile compounds in the breads produced with type I sourdough as compared to the baker’s yeast leavened steamed bread. The metabolite analysis in proofed dough indicated that esters are mainly generated by the reaction between acid and alcohol during steaming, and aldehydes are derived from the oxidation of palmitoleic acid and linoleic acid during proofing and steaming.
Reactivity of Gold(I) Monocarbene Complexes with Protein Targets: A Theoretical Study
Authors: Tolbatov Iogann , Coletti Cecilia , Marrone Alessandro , Re Nazzareno
Published: February 2019

Neutral N–heterocyclic carbene gold(I) compounds such as IMeAuCl are widely used both in homogeneous catalysis and, more recently, in medicinal chemistry as promising antitumor agents. In order to shed light on their reactivity with protein side chains, we have carried out density functional theory (DFT) calculations on the thermodynamics and kinetics of their reactions with water and various nucleophiles as a model of plausible protein binding sites such as arginine, aspartic acid, asparagine, cysteine, glutamic acid, glutamine, histidine, lysine, methionine, selenocysteine, and the N-terminal group. In agreement with recent experimental data, our results suggest that IMeAuCl easily interacts with all considered biological targets before being hydrated—unless sterically prevented—and allows the establishment of an order of thermodynamic stability and of kinetic reactivity for its binding to protein residues.
Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers
Authors: Pawelczyk Konrad , Piotrowska Aleksandra , Ciesielska Urszula , Jablonska Karolina , Glatzel-Plucinska Natalia , Grzegrzolka Jedrzej , Podhorska-Okolow Marzenna , Dziegiel Piotr , Nowinska Katarzyna
Published: February 2019

Background: The latest immunotherapy, used in the treatment of non-small cell lung cancer (NSCLC), uses monoclonal antibodies directed against programmed death ligand 1 (PD-L1) to inhibit its interaction with the PD-1 receptor. Elevated levels of PD-L1 expression were observed on NSCLC cells. The association between PD-L1 expression and clinicopathological features is still unclear. Therefore, we examined this relationship and also compare PD-L1 expression levels with Ki-67, p63 and TTF-1. Methods: 866 samples of NSCLCs were used to prepare tissue microarrays (TMAs) on which immunohistochemical (IHC) reactions were performed. Changes in the level of CD274 (PD-L1) gene expression in 62 NSCLC tumors were tested in relation to 14 normal lung tissues by real-time PCR reactions (RT-PCR). Results: PD-L1 expression was observed in 32.6% of NSCLCs. PD-L1 expression was increased in higher malignancy grades (G) (p < 0.0001) and in higher lymph node status (pN) (p = 0.0428). The patients with low PD-L1 expression had longer overall survival compared to the group with high expression (p = 0.0332) in adenocarcinoma (AC) only. Conclusions: PD-L1 expression seems to be associated with increased tumor proliferation and aggressiveness as well as shorter patient survival in NSCLC, predominantly in the AC group.
Proteomic Analysis of the Effect of Inorganic and Organic Chemicals on Silver Nanoparticles in Wheat
Authors: Jhanzab Hafiz Muhammad , Razzaq Abdul , Bibi Yamin , Yasmeen Farhat , Yamaguchi Hisateru , Hitachi Keisuke , Tsuchida Kunihiro , Komatsu Setsuko
Published: February 2019

Production and utilization of nanoparticles (NPs) are increasing due to their positive and stimulating effects on biological systems. Silver (Ag) NPs improve seed germination, photosynthetic efficiency, plant growth, and antimicrobial activities. In this study, the effects of chemo-blended Ag NPs on wheat were investigated using the gel-free/label-free proteomic technique. Morphological analysis revealed that chemo-blended Ag NPs resulted in the increase of shoot length, shoot fresh weight, root length, and root fresh weight. Proteomic analysis indicated that proteins related to photosynthesis and protein synthesis were increased, while glycolysis, signaling, and cell wall related proteins were decreased. Proteins related to redox and mitochondrial electron transport chain were also decreased. Glycolysis associated proteins such as glyceraldehyde-3-phosphate dehydrogenase increased as well as decreased, while phosphoenol pyruvate carboxylase was decreased. Antioxidant enzyme activities such as superoxide dismutase, catalase, and peroxidase were promoted in response to the chemo-blended Ag NPs. These results suggested that chemo-blended Ag NPs promoted plant growth and development through regulation of energy metabolism by suppression of glycolysis. Number of grains/spike, 100-grains weight, and yield of wheat were stimulated with chemo-blended Ag NPs. Morphological study of next generational wheat plants depicted normal growth, and no toxic effects were observed. Therefore, morphological, proteomic, yield, and next generation results revealed that chemo-blended Ag NPs may promote plant growth and development through alteration in plant metabolism.
GmZPR3d Interacts with GmHD-ZIP III Proteins and Regulates Soybean Root and Nodule Vascular Development
Authors: Damodaran Suresh , Dubois Amélie , Xie Juan , Ma Qin , Hindié Valérie , Subramanian Senthil
Published: February 2019

Fabaceans produce two major classes of symbiotic nodules: the indeterminate type characterized by a persistent meristem, and the determinate type that lacks a persistent meristem. The class III homeodomain leucine zipper (HD-ZIP III) transcription factor family influence development of multiple lateral organs and meristem maintenance, but their role in determinate nodule development is not known. HD-ZIP III protein activity is post-translationally regulated by members of the small leucine zipper protein (ZPR) family in arabidopsis. We characterized the ZPR gene family in soybean and evaluated their ability to interact with two key members of GmHD-ZIP III family through yeast two-hybrid assays. GmZPR3d displayed the strongest interaction with GmHD-ZIP III-2 among the different pairs evaluated. GmHD-ZIP III-1, -2, and GmZPR3d showed overlapping expression patterns in the root stele and in nodule parenchyma tissues. Over-expression of GmZPR3d resulted in ectopic root secondary xylem formation, and enhanced expression of vessel-specific master switch genes in soybean. The nodules in ZPR3d over-expressing roots were larger in size, had a relatively larger central zone and displayed increased nodule vascular branching. The results from this study point to a key role for GmZPR3d in soybean root and nodule development.
Influence of Structure-modifying Agents in the Synthesis of Zr-doped SBA-15 Silica and Their Use as Catalysts in the Furfural Hydrogenation to Obtain High Value-added Products through the Meerwein-Ponndorf-Verley Reduction
Authors: López-Asensio Raquel , Jiménez Gómez Carmen Pilar , García Sancho Cristina , Moreno-Tost Ramón , Cecilia Juan Antonio , Maireles-Torres Pedro
Published: February 2019

Zr-doped mesoporous silicas with different textural parameters have been synthesized in the presence of structure-modifying agents, and then characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), N2 adsorption-desorption at −196 °C, NH3 thermoprogrammed desorption (NH3–TPD), CO2 thermoprogrammed desorption (CO2–TPD), and X-ray photoelectron spectroscopy (XPS). These porous materials were evaluated in the furfural hydrogenation through the Meerwein-Ponndorf-Verley (MPV) reaction. The catalytic results indicate that the catalyst synthesized under hydrothermal conditions and adding a pore expander agent is more active and selective to furfuryl alcohol. However, the Zr-doped porous silica catalysts that were synthesized at room temperature, which possess narrow pore sizes, tend to form i-propyl furfuryl and difurfuryl ethers, coming from etherification between furfuryl alcohol (FOL) and isopropanol molecules (used as H-donor) by a SN2 mechanism.
Experimental and Numerical Determination of the Local Fiber Volume Content of Unidirectional Non-Crimp Fabrics with Forming Effects
Authors: Galkin Siegfried , Kunze Eckart , Kärger Luise , Böhm Robert , Gude Maik
Published: February 2019

Detailed knowledge of the local fiber orientation and the local fiber volume content within composite parts provides an opportunity to predict the structural behavior more reliably. Utilizing forming simulation methods of dry or pre-impregnated fabrics allows for predicting the local fiber orientation. Additionally, during the forming process, so-called draping effects like waviness, gapping or shear-induced transverse compression change the local fiber volume content. To reproduce and investigate such draping effects, different manufacturing tools have been developed in this work. The tools are used to create fabric samples with pre-defined deformation states, representing the different draping effects. The samples are evaluated regarding the resulting fiber volume content. The experimental results are compared with the predictions of an analytical solution and of a numerical solution based on draping simulation results. Furthermore, the interaction of the draping effects at arbitrary strain states is discussed regarding the resulting fiber volume content.
Fatigue Performance of Wet and Dry Pulverized Wood Flour Reinforced PP Composites
Authors: Haque Md Minhaz-Ul , Goda Koichi , Ito Hirokazu , Ogoe Shinji , Okamoto Masaki , Ema Tomoyuki , Kagawa Keiko , Nogami Hidetaka
Published: February 2019

In this paper, we exclusively studied the effects of dry and wet pulverization of different wood flours on the fatigue performance of polypropylene (PP)/wood flour (WF) composites. Wood flours obtained from cypress and Scots pine trees were pulverized in both dry and wet conditions at two different mill-plate gaps, 200 µm and 350 µm, and were used as reinforcement in PP matrices. Master batches of PP with different types of pulverized WF were compounded before processing in an extruder. The PP/WF composites of initial WF were also prepared for comparison. The prepared composites were analyzed by tensile and fatigue tests. It was found that the tensile properties of wood/polypropylene composites were affected by the pulverization of WF. Fatigue test results displayed that wet pulverization of short cypress flour had a negative effect on the fatigue life of PP/WF composites, while wet pulverization of long cypress flour and pine flour had a positive effect on the fatigue life of PP/WF composites.
On Tidal Current Velocity Vector Time Series Prediction: A Comparative Study for a French High Tidal Energy Potential Site
Authors: El Tawil Tony , Guillou Nicolas , Charpentier Jean-Frédéric , Benbouzid Mohamed
Published: February 2019

Estimating the energy potential of tidal stream site is a key feature for tidal energy system deployment. This paper aims to compare two methods of prediction of tidal current velocities. The first one is based on the use of a fully three-dimensional (3D) numerical approach. However, while being accurate, the numerical model is highly time-consuming. The second method is based on a linear approximation of the tidal current, which only requires preliminary knowledge of local current velocities time series during two typical tidal cycles. This second method allows a very quick evaluation of the tidal stream resource during a long time period. The proposed comparison is done in three different locations of a high potential tidal energy site in west of France. It is carried out in terms of current velocity and energy harnessing for several turbines technology options (with and without yaw). The achieved results show that the linear approximation gives satisfactory evaluation of the tidal stream potential and can be a very interesting tool for preliminary site evaluation and first technology options selection. However, the fully 3D numerical model can obviously be very useful in more advanced steps of a project.
On the Assessment of Numerical Wave Makers in CFD Simulations
Authors: Windt Christian , Davidson Josh , Schmitt Pál , Ringwood John V.
Published: February 2019

A fully non-linear numerical wave tank (NWT), based on Computational Fluid Dynamics (CFD), provides a useful tool for the analysis of coastal and offshore engineering problems. To generate and absorb free surface waves within a NWT, a variety of numerical wave maker (NWM) methodologies have been suggested in the literature. Therefore, when setting up a CFD-based NWT, the user is faced with the task of selecting the most appropriate NWM, which should be driven by a rigorous assessment of the available methods. To provide a consistent framework for the quantitative assessment of different NWMs, this paper presents a suite of metrics and methodologies, considering three key performance parameters: accuracy, computational requirements and available features. An illustrative example is presented to exemplify the proposed evaluation metrics, applied to the main NWMs available for the open source CFD software, OpenFOAM. The considered NWMs are found to reproduce waves with an accuracy comparable to real wave makers in physical wave tank experiments. However, the paper shows that significant differences are found between the various NWMs, and no single method performed best in all aspects of the assessment across the different test cases.
Effect of Marine Basidiomycetes Fulvifomes sp.-Derived Ergosterol Peroxide on Cytotoxicity and Apoptosis Induction in MCF-7 Cell Line
Authors: Govindharaj Mano , Arumugam Sathishkumar , Nirmala Grace , Bharadwaj Mausumi , Murugiyan Kalaiselvam
Published: February 2019

The aim of the present study is to extract the bioactive compounds which can induce the apoptosis in breast cancer cell line MCF-7 by marine basidiomycetes. Internal Transcribed Spacer (ITS) sequences based molecular taxonomic study confirmed that collected the marine basidiomycetes belongs to Fulvifomes sp. Further, the isolated compounds from the Fulvifomes sp. confirmed as ergosterol peroxide (EP) by spectroscopic studies. The compound inhibited 50% of the cell growth (IC50) at the concentration of 40 µg/mL and induced 90% cell death (IC 90) at the concentration of 80 µg/mL. The ergosterol peroxide generated Reactive Oxygen Species (ROS) and induced apoptotic cell death in MCF-7. Ethidium bromide/Acridine Orange (Et/Br) staining showed the increased number of early and late apoptosis in treated MCF-7 cells. The compounds treated cells indicated the significant loss of mitochondrial membrane potential (Δψm) with p < 0.05. The induction of apoptosis by marine basidiomycetes derived ergosterol peroxide was confirmed by chromatin condensation in MCF7 cells using Hoechst staining 33342.
Tax Competitiveness of the New EU Member States
Authors: Podviezko Askoldas , Parfenova Lyudmila , Pugachev Andrey
Published: February 2019

This paper investigates tax competitiveness among the EU member countries. The tax competition of countries causes both positive and negative effects on macroeconomic processes such as the effectiveness of government spending, the rationality of supply of externalities, and the length and amplitudes of business cycles. A considerable reduction of corporate tax in the EU is related to increased tax competition after new members entered the EU. Multiple criteria methods were chosen for the quantitative evaluation of EU countries from different regions of the EU. Criteria of evaluation were chosen and structured into a hierarchy. The convergence process of the new members of the EU is reinforced with the increasing tax competitiveness of such countries. Results of the multiple criteria evaluation revealed both the factors that increased the tax competitiveness of new members of the EU, and outlined the factors that hampered such competition.
An Interdisciplinary Review of Smart Vehicular Traffic and Its Applications and Challenges
Authors: Fiore Ugo , Florea Adrian , Pérez Lechuga Gilberto
Published: February 2019

Sensors and intelligent applications enabling smart vehicular traffic create an opportunity for improving the welfare of people, from the viewpoints of efficiency, sustainability, and social inclusivity. Like the opportunities, challenges of such an endeavour are multifaceted, including the scalable collection and processing of the hefty data volumes generated by sensors, and the coordinated operation between selfish agents. The purpose of this work is to survey recent literature with an emphasis on applications and a multidisciplinary eye, with the aim of stimulating discussion and reflection in the scientific communities. The principal application areas of smart traffic and smart mobility are discussed, synthesizing different perspectives. Many intriguing areas for future research exist besides those relative to connectivity, data fusion, and privacy. Some research challenges pertinent to sustainability, insurance, simulation and the handling of ambiguous information are highlighted.
Preliminaries of Orthogonal Layered Defence Using Functional and Assurance Controls in Industrial Control Systems
Authors: Mackintosh Mike , Epiphaniou Gregory , Al-Khateeb Haider , Burnham Keith , Pillai Prashant , Hammoudeh Mohammad
Published: February 2019

Industrial Control Systems (ICSs) are responsible for the automation of different processes and the overall control of systems that include highly sensitive potential targets such as nuclear facilities, energy-distribution, water-supply, and mass-transit systems. Given the increased complexity and rapid evolvement of their threat landscape, and the fact that these systems form part of the Critical National infrastructure (CNI), makes them an emerging domain of conflict, terrorist attacks, and a playground for cyberexploitation. Existing layered-defence approaches are increasingly criticised for their inability to adequately protect against resourceful and persistent adversaries. It is therefore essential that emerging techniques, such as orthogonality, be combined with existing security strategies to leverage defence advantages against adaptive and often asymmetrical attack vectors. The concept of orthogonality is relatively new and unexplored in an ICS environment and consists of having assurance control as well as functional control at each layer. Our work seeks to partially articulate a framework where multiple functional and assurance controls are introduced at each layer of ICS architectural design to further enhance security while maintaining critical real-time transfer of command and control traffic.
Tomato-Processing By-Product Combustion: Thermal and Kinetic Analyses
Authors: Khiari Besma , Moussaoui Marwa , Jeguirim Mejdi
Published: February 2019

This paper is part of a sustainable development approach, the aim being to develop a thermochemical energy recovery path while reducing the amount of tomato waste issued from agro-industrial units. The thermal process may contribute to an environmentally friendly management and help tomato processing industries creating new economic profitable circuits in an increasingly competitive context. The adopted approach was to follow the operating conditions needed for a complete thermal degradation through a thermal and kinetic analyses. The results of the tomato waste characterization confirmed their suitability to a thermochemical processing with high volatiles and fixed carbon and interesting high heating values comparable to sawdust biomass. We were able to isolate of the decomposition domains and extract kinetic parameters. Three kinetic models were applied; Flynn–Wall–Ozawa (FWO) simulated the best the combustion process. Calculated curves were validated by the first order (n = 1) model except for the slow heating rate of 5 °C/min which was fitted by the contracted cylinder model. The conclusions of this paper could help in optimizing the combustion process in order to achieve high energy recovery from tomato residues. Obtained kinetic data would help in the design of combustion reactors.
Simultaneous Spark Plasma Sintering of Multiple Complex Shapes
Authors: Manière Charles , Torresani Elisa , Olevsky Eugene A.
Published: February 2019

This work addresses the two great challenges of the spark plasma sintering (SPS) process: The sintering of complex shapes and the simultaneous production of multiple parts. A new controllable interface method is employed to concurrently consolidate two nickel gear shapes by SPS. A graphite deformable sub-mold is specifically designed for the mutual densification of both complex parts in a unique 40 mm powder deformation space. An energy efficient SPS configuration is developed to allow the sintering of a large-scale powder assembly under electric current lower than 900 A. The stability of the developed process is studied by electro-thermal-mechanical (ETM) simulation. The ETM simulation reveals that homogeneous densification conditions can be attained by inserting an alumina powder at the sample/punches interfaces, enabling the energy efficient heating and the thermal confinement of the nickel powder. Finally, the feasibility of the fabrication of the two near net shape gears with a very homogeneous microstructure is demonstrated.
Formulation and Characterization of New Polymeric Systems Based on Chitosan and Xanthine Derivatives with Thiazolidin-4-One Scaffold
Authors: Constantin Sandra Madalina , Buron Frederic , Routier Sylvain , Vasincu Ioana Mirela , Apotrosoaei Maria , Lupașcu Florentina , Confederat Luminița , Tuchilus Cristina , Constantin Marta Teodora , Sava Alexandru , Profire Lenuţa
Published: February 2019

In the past many research studies have focused on the thiazolidine-4-one scaffold, due to the important biological effects associated with its heterocycle. This scaffold is present in the structure of many synthetic compounds, which showed significant biological effects such as antimicrobial, antifungal, antioxidant, anti-inflammatory, analgesic, antidiabetic effects. It was also identified in natural compounds, such as actithiazic acid, isolated from Streptomyces strains. Starting from this scaffold new xanthine derivatives have been synthetized and evaluated for their antibacterial and antifungal effects. The antibacterial action was investigated against Gram positive (Staphyloccoccus aureus ATCC 25923, Sarcina lutea ATCC 9341) and Gram negative (Escherichia coli ATCC 25922) bacterial strains. The antifungal potential was investigated against Candida spp. (Candida albicans ATCC 10231, Candida glabrata ATCC MYA 2950, Candida parapsilosis ATCC 22019). In order to improve the antimicrobial activity, the most active xanthine derivatives with thiazolidine-4-one scaffold (XTDs: 6c, 6e, 6f, 6k) were included in a chitosan based polymeric matrix (CS). The developed polymeric systems (CS-XTDs) were characterized in terms of morphological (aspect, particle size), physic-chemical properties (swelling degree), antibacterial and antifungal activities, toxicity, and biological functions (bioactive compounds loading, entrapment efficiency). The presence of xanthine-thiazolidine-4-one derivatives into the chitosan matrix was confirmed using Fourier transform infrared (FT-IR) analysis. The size of developed polymeric systems, CS-XTDs, ranged between 614 µm and 855 µm, in a dry state. The XTDs were encapsulated into the chitosan matrix with very good loading efficiency, the highest entrapment efficiency being recorded for CS-6k, which ranged between 87.86 ± 1.25% and 93.91 ± 1.41%, depending of the concentration of 6k. The CS-XTDs systems showed an improved antimicrobial effect with respect to the corresponding XTDs. Good results were obtained for CS-6f, for which the effects on Staphylococcus aureus ATCC 25923 (21.2 ± 0.43 mm) and Sarcina lutea ATCC 9341 (25.1 ± 0.28 mm) were comparable with those of ciprofloxacin (25.1 ± 0.08 mm/25.0 ± 0.1 mm), which were used as the control. The CS-6f showed a notable antifungal effect, especially on Candida parapsilosis ATCC 22019 (18.4 ± 0.42 mm), the effect being comparable to those of nystatin (20.1 ± 0.09 mm), used as the control. Based on the obtained results these polymeric systems, consisting of thiazolidine-4-one derivatives loaded with chitosan microparticles, could have important applications in the food field as multifunctional (antimicrobial, antifungal, antioxidant) packaging materials.
Improvement of Transparencies and Mechanical Properties of Poly(cyclohexylene dimethylene cyclohexanedicarboxylate) Parts Using a Compounding Nucleating Agent to Control Crystallization
Authors: Su Bei , Zhou Ying-Guo
Published: February 2019

Poly(cyclohexylene dimethylene cyclohexanedicarboxylate) (PCCE) is a kind of copolyester polymer with excellent toughness and outstanding flexibility. However, the opacity caused by crystallization limits the widespread application of PCCE in products that have transparency requirements. The effects of 1,3:2,4-Di-p-methylbenzylidene sorbitol (MDBS) on the crystallization behavior, transparency, and mechanical properties of a PCCE melt were investigated via differential scanning calorimetry (DSC), spectrophotometry, and tensile testing. The results suggest that the transparency and mechanical properties of PCCE drastically improve and that its crystallization behaviors are obviously influenced by the addition of MDBS. PCCE with 0.6 wt% MDBS was then selected as a representative sample, and its thermal behavior and crystal morphology were further investigated by DSC, hot-staged polarizing microscopy (HSPLM), and scanning electron microscopy (SEM). The quantitative results suggest that, compared to neat PCCE resin, PCCE/MDBS has a lower isothermal and nonisothermal crystallization activation energy, which indicates a rapid crystallization process. The results also show that, compared to the pure PCCE melt, the PCCE/MDBS melt experiences a greater increase in the number of crystals and a greater decrease in the crystal size during cooling. The acceleration of the crystallization process and reduction in crystal size can be both attributed to the nucleation effect of the MDBS. In conclusion, because the addition of the nucleating agent improves the transparency and tensile properties of PCCE by adjusting and controlling its thermal and crystallization behaviors, the proposed technique of using a compounding nucleating agent to control crystallization is therefore suitable for PCCE.
Fabrication of Scaffolds for Bone-Tissue Regeneration
Authors: Chocholata Petra , Kulda Vlastimil , Babuska Vaclav
Published: February 2019

The present article describes the state of the art in the rapidly developing field of bone tissue engineering, where many disciplines, such as material science, mechanical engineering, clinical medicine and genetics, are interconnected. The main objective is to restore and improve the function of bone tissue by scaffolds, providing a suitable environment for tissue regeneration and repair. Strategies and materials used in oral regenerative therapies correspond to techniques generally used in bone tissue engineering. Researchers are focusing on developing and improving new materials to imitate the native biological neighborhood as authentically as possible. The most promising is a combination of cells and matrices (scaffolds) that can be fabricated from different kinds of materials. This review summarizes currently available materials and manufacturing technologies of scaffolds for bone-tissue regeneration.
Investigation of the Itinerant Electron Ferromagnetism of Ni2+xMnGa1−x and Co2VGa Heusler Alloys
Authors: Sakon Takuo , Hayashi Yuhi , Fukuya Akihito , Li Dexin , Honda Fuminori , Umetsu Rie Y. , Xu Xiao , Oomi Gendo , Kanomata Takeshi , Eto Tetsujiro
Published: February 2019

Experimental investigations into the field dependence of magnetization and temperature dependences of magnetic susceptibility in Ni2+xMnGa1−x (x = 0.00, 0.02, 0.04) and Co2VGa Heusler alloy ferromagnets were performed following the spin fluctuation theory of itinerant ferromagnetism, called as “Takahashi theory”. We investigated the magnetic field dependence of magnetization at the Curie temperature TC, which is the critical temperature of the ferromagnetic–paramagnetic transition, and also at T = 5 K, which concerns the ground state of the ferromagnetic state. The field dependence of the magnetization was analyzed by means of the H vs. M5 dependence, and the field dependence of the ground state at 5 K was investigated by means of an Arrott plot (H/M vs. M2) according to the Takahashi theory. As for Ni2+xMnGa1−x, the spin fluctuation parameter in k-space (momentum space, TA) and that in energy space (T0) obtained at TC and 5 K were almost the same. On the contrary, as for Co2VGa, the H vs. M5 dependence was not shown at TC. We obtained TA and T0 by means of an Arrott plot at 5 K. We created a generalized Rhodes–Wohlfarth plot of peff/pS versus TC/T0 for the other ferromagnets. The plot indicated that the relationship between peff/pS and T0/TC followed Takahashi’s theory. We also discussed the spontaneous magnetic moment at the ground state, pS, which was obtained by an Arrott plot at 5 K and the high temperature magnetic moment, pC, at the paramagnetic phase. As for the localized ferromagnet, the pC/pS was 1. As for weak ferromagnets, the pC/pS was larger than 1. In contrast, the pC/pS was smaller than 1 by many Heusler alloys. This is a unique property of Heusler ferromagnets. Half-metallic ferromagnets of Co2VGa and Co2MnGa were in accordance with the generalized Rhodes–Wohlfarth plot with a km around 1.4. The magnetic properties of the itinerant electron of these two alloys appeared in the majority bands and was confirmed by Takahashi’s theory.
Mechanical Performance of Jute Fiber-Reinforced Micaceous Clay Composites Treated with Ground-Granulated Blast-Furnace Slag
Authors: Zhang Jiahe , Soltani Amin , Deng An , Jaksa Mark B.
Published: February 2019

The combined capacity of Jute Fibers (JF), the reinforcement, and Ground-Granulated Blast-Furnace Slag (GBFS), the binder, was examined as a sustainable solution towards ameliorating the inferior engineering properties of micaceous clays. A total of sixteen JF + GBFS mix designs, i.e., JF (% by total mass) = {0, 0.5, 1.0, 1.5} and GBFS (% by total mass) = {0, 3, 6, 9}, were tested for unconfined compression (UC) strength; for those mix designs containing GBFS, curing was allowed for 7 and 28 days prior to testing. Scanning electron microscopy (SEM) studies were also carried out to observe the evolution of fabric in response to JF, GBFS and JF + GBFS amendments. The greater the JF content the higher the developed strength and stiffness up to 1% JF, beyond of which the effect of JF-reinforcement led to some adverse results. The JF inclusions, however, consistently improved the ductility and toughness of the composite. The addition of GBFS to the JF-reinforced samples improved the soil–fiber connection interface, and thus led to further improvements in the composite’s strength, stiffness and toughness. The mix design “1% JF + 9% GBFS” managed to satisfy ASTM’s strength criterion and hence was deemed as the optimum choice in this investigation. Finally, a non-linear, multivariable regression model was developed and validated to quantify the peak UC strength as a function of the composite’s index properties. The proposed model contained a limited number of fitting parameters, all of which can be calibrated by little experimental effort, and thus implemented for preliminary design assessments.
Steam Oxidation of Austenitic Heat-Resistant Steels TP347H and TP347HFG at 650–800 °C
Authors: Liang Zhiyuan , Zhao Qinxin
Published: February 2019

Steam oxidation of austenitic heat-resistant steels TP347H and TP347HFG at 650–800 °C was investigated. Comprehensive micro-characterization technologies containing Scanning Electron Microscope (SEM), Energy Dispersive X-ray Spectroscopy (EDS), X-ray Diffraction (XRD), and X-ray Photoelectron Spectroscopy (XPS) were employed to observe and analyze the oxidation products. Results show that breakaway oxidation behaviors were observed on TP347H at 700 °C and 800 °C. The oxidation kinetics of TP347HFG at 650–800 °C followed a parabolic law. The oxide scales formed on TP347HFG were composed of MnCr2O4 and Cr2O3. A thin and protective Cr-rich oxide scale was replaced by Fe2O3 nodules due to the insufficient outward migration of metallic ions, including Cr and Mn at the subsurface of coarse-grain TP347H. Smaller grain of TP347HFG promoted the formation of the compact Cr-rich oxide scales. At higher temperatures, the incubation period for breakaway oxidation of the Cr-rich oxide scale was much shorter because of quick evaporation of the Cr2O3 oxide scale and the slower outward diffusion of metallic ions via the grain boundaries.