bluuebunny/crossref_metadata_2025 huggingface dataset:
HPLC-DAD and UHPLC/QTOF-MS Analysis of Polyphenols in Extracts of the African Species Combretum padoides, C. zeyheri and C. psidioides Related to Their Antimycobacterial Activity
Authors: Fyhrquist Pia , Salih Enass Y. A. , Helenius Satu , Laakso Into , Julkunen-Tiitto Riitta
Published: July 2020

Combretum padoides Engl. & Diels, C. psidioides Welv. and C. zeyheri Sond. are used for the treatment of infections and tuberculosis related symptoms in African traditional medicine. In order to verify these uses, extracts were screened for their growth inhibitory effects against M. smegmatis ATCC 14468. Ultra-high pressure liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS) and GC-MS were used to investigate the polyphenolic composition in the active extracts. The lowest minimum inhibitory concentration (MIC), 625 µg/mL, was shown by a methanol extract of the stem bark of C. psidioides. A butanol extract of C. psidioides gave large inhibition zone diameters (IZD 21 mm) and inhibited 84% of the mycobacterial growth at 312 µg/mL. Combretastatin B-2 and dihydrostilbene derivatives were present in the methanol extract of C. psidioides, whereas the butanol extract of this species contained punicalagin, corilagin, and sanguiin H-4. Methanol and butanol extracts of the stem bark of C. padoides gave large inhibition zone diameters (IZD 26.5 mm) and MIC values of 1250 and 2500 µg/mL, respectively. C. padoides contained an ellagitannin with a mass identical to punicalagin ([M-H]− 1083.0587) and a corilagin like derivative ([M-H]− 633.0750) as well as ellagic acid arabinoside and methyl ellagic acid xyloside. A butanol extract of the roots of C. zeyheri showed mild antimycobacterial activity and contained a gallotannin at m/z [M-H]− 647.0894 as the main compound along with punicalagin and three unknown ellagitannins at m/z [M-H]− 763.0788, 765.0566, and 817.4212. Our results indicate that the studied species of Combretum contain phenolic and polyphenolic compounds with possible potential as leads for antimycobacterial drugs or as adjuvants for conventional anti-TB drugs.
Bismuth Nanoantibiotics Display Anticandidal Activity and Disrupt the Biofilm and Cell Morphology of the Emergent Pathogenic Yeast Candida auris
Authors: Vazquez-Munoz Roberto , Lopez Fernando D. , Lopez-Ribot Jose L.
Published: July 2020

Candida auris is an emergent multidrug-resistant pathogenic yeast, which forms biofilms resistant to antifungals, sanitizing procedures, and harsh environmental conditions. Antimicrobial nanomaterials represent an alternative to reduce the spread of pathogens—including yeasts—regardless of their drug-resistant profile. Here we have assessed the antimicrobial activity of easy-to-synthesize bismuth nanoparticles (BiNPs) against the emergent multidrug-resistant yeast Candida auris, under both planktonic and biofilm growing conditions. Additionally, we have examined the effect of these BiNPs on cell morphology and biofilm structure. Under planktonic conditions, BiNPs MIC values ranged from 1 to 4 µg mL−1 against multiple C. auris strains tested, including representatives of all different clades. Regarding the inhibition of biofilm formation, the calculated BiNPs IC50 values ranged from 5.1 to 113.1 µg mL−1. Scanning electron microscopy (SEM) observations indicated that BiNPs disrupted the C. auris cell morphology and the structure of the biofilms. In conclusion, BiNPs displayed strong antifungal activity against all strains of C. auris under planktonic conditions, but moderate activity against biofilm growth. BiNPs may potentially contribute to reducing the spread of C. auris strains at healthcare facilities, as sanitizers and future potential treatments. More research on the antimicrobial activity of BiNPs is warranted.
Diabetes Upregulates Oxidative Stress and Downregulates Cardiac Protection to Exacerbate Myocardial Ischemia/Reperfusion Injury in Rats
Authors: Chien Chen-Yen , Wen Ting-Jui , Cheng Yu-Hsiuan , Tsai Yi-Ting , Chiang Chih-Yao , Chien Chiang-Ting
Published: July 2020

Diabetes exacerbates myocardial ischemia/reperfusion (IR) injury by incompletely understood mechanisms. We explored whether diabetes diminished BAG3/Bcl-2/Nrf-2/HO-1-mediated cardioprotection and overproduced oxidative stress contributing to exaggerated IR injury. Streptozotocin-induced diabetes enhanced hyperglycemia, cardiac NADPH oxidase p22/p67 expression, malondialdehyde amount and leukocyte infiltration, altered the mesenteric expression of 4-HNE, CaSR, p-eNOS and BAG3 and impaired microvascular reactivity to the vasoconstrictor/vasodilator by a wire myography. In response to myocardial IR, diabetes further depressed BAG3/Bcl-2/Nrf-2/HO-1 expression, increased cleaved-caspase 3/poly(ADP-ribose) polymerase (PARP)/TUNEL-mediated apoptosis and exacerbated IR-induced left ventricular dysfunction characterized by further depressed microcirculation, heart rate, left ventricular systolic pressure and peak rate of pressure increase/decrease (±dp/dt) and elevated left ventricular end-diastolic pressure (LVEDP) and Evans blue-2,3,5-triphenyltetrazolium chloride-stained infarct size in diabetic hearts. Our results implicated diabetes exacerbated IR-induced myocardial dysfunction through downregulated BAG3/Bcl-2/Nrf-2/HO-1 expression, increased p22/p67/caspase 3/PARP/apoptosis-mediated oxidative injury and impaired microvascular reactivity.
Comparative Study of Protective Action of Exogenous 2-Cys Peroxiredoxins (Prx1 and Prx2) Under Renal Ischemia-Reperfusion Injury
Authors: Sharapov Mars G. , Goncharov Ruslan G. , Filkov Gleb I. , Trofimenko Alexander V. , Boyarintsev Valery V. , Novoselov Vladimir I.
Published: July 2020

The pathogenesis of ischemia-reperfusion (I/R) injuries is based on oxidative stress caused by a sharp increase in the concentration of free radicals, reactive oxygen species (ROS) and secondary products of free radical oxidation of biological macromolecules during reperfusion. Application of exogenous antioxidants lowers the level of ROS in the affected tissues, suppresses or adjusts the course of oxidative stress, thereby substantially reducing the severity of I/R injury. We believe that the use of antioxidant enzymes may be the most promising line of effort since they possess higher efficiency than low molecular weight antioxidants. Among antioxidant enzymes, of great interest are peroxiredoxins (Prx1–6) which reduce a wide range of organic and inorganic peroxide substrates. In an animal model of bilateral I/R injury of kidneys (using histological, biochemical, and molecular biological methods) it was shown that intravenous administration of recombinant typical 2-Cys peroxiredoxins (Prx1 and Prx2) effectively reduces the severity of I/R damage, contributing to the normalization of the structural and functional state of the kidneys and an almost 2-fold increase in the survival of experimental animals. The use of recombinant Prx1 or Prx2 can be an efficient approach for the prevention and treatment of renal I/R injury.
Reactive Oxygen Species and Antioxidant Defense in Plants under Abiotic Stress: Revisiting the Crucial Role of a Universal Defense Regulator
Authors: Hasanuzzaman Mirza , Bhuyan M.H.M. , Zulfiqar Faisal , Raza Ali , Mohsin Sayed , Mahmud Jubayer , Fujita Masayuki , Fotopoulos Vasileios
Published: July 2020

Global climate change and associated adverse abiotic stress conditions, such as drought, salinity, heavy metals, waterlogging, extreme temperatures, oxygen deprivation, etc., greatly influence plant growth and development, ultimately affecting crop yield and quality, as well as agricultural sustainability in general. Plant cells produce oxygen radicals and their derivatives, so-called reactive oxygen species (ROS), during various processes associated with abiotic stress. Moreover, the generation of ROS is a fundamental process in higher plants and employs to transmit cellular signaling information in response to the changing environmental conditions. One of the most crucial consequences of abiotic stress is the disturbance of the equilibrium between the generation of ROS and antioxidant defense systems triggering the excessive accumulation of ROS and inducing oxidative stress in plants. Notably, the equilibrium between the detoxification and generation of ROS is maintained by both enzymatic and nonenzymatic antioxidant defense systems under harsh environmental stresses. Although this field of research has attracted massive interest, it largely remains unexplored, and our understanding of ROS signaling remains poorly understood. In this review, we have documented the recent advancement illustrating the harmful effects of ROS, antioxidant defense system involved in ROS detoxification under different abiotic stresses, and molecular cross-talk with other important signal molecules such as reactive nitrogen, sulfur, and carbonyl species. In addition, state-of-the-art molecular approaches of ROS-mediated improvement in plant antioxidant defense during the acclimation process against abiotic stresses have also been discussed.
Sex Difference Impacts on the Relationship between Paraoxonase-1 (PON1) and Type 2 Diabetes
Authors: Rosta Valentina , Trentini Alessandro , Passaro Angelina , Zuliani Giovanni , Sanz Juana Maria , Bosi Cristina , Bonaccorsi Gloria , Bellini Tiziana , Cervellati Carlo
Published: July 2020

Type-2 diabetes (T2D) and its cardiovascular complications are related to sex. Increasing evidence suggests that paraoxonase 1 (PON1) activity, an antioxidant enzyme bound to high-density lipoproteins (HDL), is implicated in the onset and clinical progression of T2D. Since we previously showed that PON1 is a sexual dimorphic protein, we now investigated whether sex might impact the relationship between PON1 and this chronic disease. To address this aim, we assessed PON1 activity in the sera of 778 patients, including controls (women, n = 383; men, n = 198) and diabetics (women, n = 79; men = 118). PON1 activity decreased in both women and men with T2D compared with controls (p < 0.05 and p > 0.001, respectively), but the change was 50% larger in the female cohort. In line with this result, the enzyme activity was associated with serum glucose level only in women (r = −0.160, p = 0.002). Notably, only within this gender category, lower PON1 activity was independently associated with increased odds of being diabetic (odds ratio (95% Confidence interval: 2.162 (1.075–5.678)). In conclusion, our study suggests that PON1-deficiency in T2D is a gender-specific phenomenon, with women being more affected than men. This could contribute to the partial loss of female cardiovascular advantage associated with T2D.
Research and Improvement of the Hydraulic Suspension System for a Heavy Hydraulic Transport Vehicle
Authors: Wang Jianjun , Zhao Jingyi , Li Wenlei , Jia Xing , Wei Peng
Published: July 2020

In order to ensure the ride comfort of a hydraulic transport vehicle in transportation, it is important to account for the effects of the suspension system. In this paper, an improved hydraulic suspension system based on a reasonable setting of the accumulator was proposed for a heavy hydraulic transport vehicle. The hydraulic transport vehicle was a multi-degree nonlinear system, and the establishment of an appropriate vehicle dynamical model was the basis for the improvement of the hydraulic suspension system. The hydraulic suspension system was analyzed, and a mathematical model of the hydraulic suspension system with accumulator established and then analyzed. The results revealed that installing the appropriate accumulator can absorb the impact pressure on the vehicle, while a hydraulic suspension system with an accumulator can be designed. Further, it was proved that a reasonable setting for the accumulator can reduce the impact force on the transport vehicle through simulation, and the optimal accumulator parameters can be obtained. Finally, an experiment in the field was set up and carried out, and the experimental results presented to prove the viability of the proposed method.
Lateral Migration in a Wandering Reach of the Middle Yellow River in Response to Different Boundary Conditions
Authors: Li Jie , Zhang Yi , Ji Qingfeng
Published: July 2020

The Xiaobeiganliu reach is a typical wandering reach of the Middle Yellow River that has rapid and significant channel lateral migration, which may threaten the safety of riparian land and flood control structures. To investigate the characteristics and mechanism of lateral migration in the Xiaobeiganliu reach, the temporal and spatial variations in bankfull width and thalweg migration distance were identified during the continuous deposition period, quantitatively analyzing the effect of different boundary conditions on the lateral migration index. The reach-scale bankfull width decreased by 32% from 1986 to 2001 because hyperconcentrated floods often occurred in this reach. The thalweg migration distance varied dramatically at cross-sections, with the maximum annual thalweg migration distance reaching 4290 m. The lateral migration index of the Xiaobeiganliu reach responded well to the upstream and downstream boundary conditions. The previous 3-year average water discharge and 4-year average sediment concentration at the upstream station were two key fluvial factors influencing lateral migration, with the relation being established between the lateral migration index and the two parameters. The Tongguan (TG) elevation was the key downstream boundary condition affecting thalweg migration, and a power function was proposed between the lateral migration index and the variations in annual TG elevation.
Artificial Intelligence-Based Model for the Prediction of Dynamic Modulus of Stone Mastic Asphalt
Authors: Le Thanh-Hai , Nguyen Hoang-Long , Pham Binh Thai , Nguyen May Huu , Pham Cao-Thang , Nguyen Ngoc-Lan , Le Tien-Thinh , Ly Hai-Bang
Published: July 2020

Stone Mastic Asphalt (SMA) is a tough, stable, rut-resistant mixture that takes advantage of the stone-to-stone contact to provide strength and durability for the material. Besides, the warm mix asphalt (WMA) technology allows reducing emissions and energy consumption by reducing the production temperature by 30–50 °C, compared to conventional hot mix asphalt technology (HMA). The dynamic modulus |E*| has been acknowledged as a vital material property in the mechanistic-empirical design and analysis and further reflects the strains and displacements of such layered pavement structures. The objective of this study is twofold, aiming at favoring the potential use of SMA with WMA technique. To this aim, first, laboratory tests were conducted to compare the performance of SMA and HMA through the dynamic modulus. Second, an advanced hybrid artificial intelligence technique to accurately predict the dynamic modulus of asphalt mixtures was developed. This hybrid model (ANN-TLBO) was based on an Artificial Neural Network (ANN) algorithm and Teaching Learning Based Optimization (TLBO) technique. A database containing the as-obtained experimental tests (96 data) was used for the development and assessment of the ANN-TLBO model. The experimental results showed that SMA mixtures exhibited higher values of the dynamic modulus |E*| than HMA, and the WMA technology increased the dynamic modulus values compared with the hot technology. Furthermore, the proposed hybrid algorithm could successfully predict the dynamic modulus with remarkable values of R2 of 0.989 and 0.985 for the training and testing datasets, respectively. Lastly, the effects of temperature and frequency on the dynamic modulus were evaluated and discussed.
Autonomous Active Tag Using Energy Harvesting Strategies
Authors: Carretero Ana , Real Santiago , Araujo Alvaro
Published: July 2020

In this work, we present autonomous active tags. The power sources of these active tags employ energy harvesting techniques, specifically, solar and mechanical techniques. The integration of these techniques, and the storage of the energy obtained with a supercapacitor, converts the active tag into an autonomous device. These tags work in a low power mode in which they dynamically adjust their radio communication capabilities. Such a configuration depends on the application. We tested the tags in a real environment with testing parameters to check the modules, meaning more wake-ups over a longer time. Under these conditions, the tags gather enough energy to autonomously maintain standby operation on a sunny day for ten hours. In conclusion, this autonomous active tag is a demonstration that the integration of energy harvesting techniques, supercapacitor storage and the management of low power modes for transceivers, microcontrollers, and memories creates a device without energy dependencies that only depends on the requirements, and can be used in many applications related to, for instance, smart homes, smart cities, smart cars, and connected forests.
Cognitive Stimulation Induces Differential Gene Expression in Octopus vulgaris: The Key Role of Protocadherins
Authors: Maselli Valeria , Polese Gianluca , Al-Soudy Al-Sayed , Buglione Maria , Di Cosmo Anna
Published: July 2020

Octopuses are unique invertebrates, with sophisticated and flexible behaviors controlled by a high degree of brain plasticity, learning, and memory. Moreover, in Octopus vulgaris, it has been demonstrated that animals housed in an enriched environment show adult neurogenesis in specific brain areas. Firstly, we evaluated the optimal acclimatization period needed for an O. vulgaris before starting a cognitive stimulation experiment. Subsequently, we analyzed differential gene expression in specific brain areas in adult animals kept in tested (enriched environment), wild (naturally enriched environment), and control conditions (unenriched environment). We selected and sequenced three protocadherin genes (PCDHs) involved in the development and maintenance of the nervous system; three Pax genes that control cell specification and tissue differentiation; the Elav gene, an earliest marker for neural cells; and the Zic1 gene, involved in early neural formation in the brain. In this paper, we evaluated gene expression levels in O. vulgaris under different cognitive stimulations. Our data shows that Oct-PCDHs genes are upregulated in the learning and lower motor centers in the brain of both tested and wild animals (higher in the latter). Combining these results with our previous studies on O. vulgaris neurogenesis, we proposed that PCDH genes may be involved in adult neurogenesis processes, and related with their cognitive abilities.
Predicted Hotspot Residues Involved in Allosteric Signal Transmission in Pro-Apoptotic Peptide—Mcl1 Complexes
Authors: Marimuthu Parthiban , Razzokov Jamoliddin , Singaravelu Kalaimathy , Bogaerts Annemie
Published: July 2020

Mcl1 is a primary member of the Bcl–2 family—anti–apoptotic proteins (AAP)—that is overexpressed in several cancer pathologies. The apoptotic regulation is mediated through the binding of pro-apoptotic peptides (PAPs) (e.g., Bak and Bid) at the canonical hydrophobic binding groove (CBG) of Mcl1. Although all PAPs form amphipathic α-helices, their amino acid sequences vary to different degree. This sequence variation exhibits a central role in the binding partner selectivity towards different AAPs. Thus, constructing a novel peptide or small organic molecule with the ability to mimic the natural regulatory process of PAP is essential to inhibit various AAPs. Previously reported experimental binding free energies (BFEs) were utilized in the current investigation aimed to understand the mechanistic basis of different PAPs targeted to mMcl1. Molecular dynamics (MD) simulations used to estimate BFEs between mMcl1—PAP complexes using Molecular Mechanics-Generalized Born Solvent Accessible (MMGBSA) approach with multiple parameters. Predicted BFE values showed an excellent agreement with the experiment (R2 = 0.92). The van–der Waals (ΔGvdw) and electrostatic (ΔGele) energy terms found to be the main energy components that drive heterodimerization of mMcl1—PAP complexes. Finally, the dynamic network analysis predicted the allosteric signal transmission pathway involves more favorable energy contributing residues. In total, the results obtained from the current investigation may provide valuable insights for the synthesis of a novel peptide or small organic inhibitor targeting Mcl1.
Implication of Hyperhomocysteinemia in Blood Retinal Barrier (BRB) Dysfunction
Authors: Tawfik Amany , Samra Yara A. , Elsherbiny Nehal M. , Al-Shabrawey Mohamed
Published: July 2020

Elevated plasma homocysteine (Hcy) level, known as hyperhomocysteinemia (HHcy) has been linked to different systemic and neurological diseases, well-known as a risk factor for systemic atherosclerosis and cardiovascular disease (CVD) and has been identified as a risk factor for several ocular disorders, such as diabetic retinopathy (DR) and age-related macular degeneration (AMD). Different mechanisms have been proposed to explain HHcy-induced visual dysfunction, including oxidative stress, upregulation of inflammatory mediators, retinal ganglion cell apoptosis, and extracellular matrix remodeling. Our previous studies using in vivo and in vitro models of HHcy have demonstrated that Hcy impairs the function of both inner and outer blood retinal barrier (BRB). Dysfunction of BRB is a hallmark of vision loss in DR and AMD. Our findings highlighted oxidative stress, ER stress, inflammation, and epigenetic modifications as possible mechanisms of HHcy-induced BRB dysfunction. In addition, we recently reported HHcy-induced brain inflammation as a mechanism of blood–brain barrier (BBB) dysfunction and pathogenesis of Alzheimer’s disease (AD). Moreover, we are currently investigating the activation of glutamate receptor N-methyl-d-aspartate receptor (NMDAR) as the molecular mechanism for HHcy-induced BRB dysfunction. This review focuses on the studied effects of HHcy on BRB and the controversial role of HHcy in the pathogenesis of aging neurological diseases such as DR, AMD, and AD. We also highlight the possible mechanisms for such deleterious effects of HHcy.
Glycine in Water Favors the Polyproline II State
Authors: Andrews Brian , Zhang Shuting , Schweitzer-Stenner Reinhard , Urbanc Brigita
Published: July 2020

Conformational preferences of amino acid residues in water are determined by the backbone and side-chain properties. Alanine is known for its high polyproline II (pPII) propensity. The question of relative contributions of the backbone and side chain to the conformational preferences of alanine and other amino acid residues in water is not fully resolved. Because glycine lacks a heavy-atom side chain, glycine-based peptides can be used to examine to which extent the backbone properties affect the conformational space. Here, we use published spectroscopic data for the central glycine residue of cationic triglycine in water to demonstrate that its conformational space is dominated by the pPII state. We assess three commonly used molecular dynamics (MD) force fields with respect to their ability to capture the conformational preferences of the central glycine residue in triglycine. We show that pPII is the mesostate that enables the functional backbone groups of the central residue to form the most hydrogen bonds with water. Our results indicate that the pPII propensity of the central glycine in GGG is comparable to that of alanine in GAG, implying that the water-backbone hydrogen bonding is responsible for the high pPII content of these residues.
Regulation of Protein Post-Translational Modifications on Metabolism of Actinomycetes
Authors: Sun Chen-Fan , Li Yong-Quan , Mao Xu-Ming
Published: July 2020

Protein post-translational modification (PTM) is a reversible process, which can dynamically regulate the metabolic state of cells through regulation of protein structure, activity, localization or protein–protein interactions. Actinomycetes are present in the soil, air and water, and their life cycle is strongly determined by environmental conditions. The complexity of variable environments urges Actinomycetes to respond quickly to external stimuli. In recent years, advances in identification and quantification of PTMs have led researchers to deepen their understanding of the functions of PTMs in physiology and metabolism, including vegetative growth, sporulation, metabolite synthesis and infectivity. On the other hand, most donor groups for PTMs come from various metabolites, suggesting a complex association network between metabolic states, PTMs and signaling pathways. Here, we review the mechanisms and functions of PTMs identified in Actinomycetes, focusing on phosphorylation, acylation and protein degradation in an attempt to summarize the recent progress of research on PTMs and their important role in bacterial cellular processes.
The Development of a Skin Cancer Classification System for Pigmented Skin Lesions Using Deep Learning
Authors: Jinnai Shunichi , Yamazaki Naoya , Hirano Yuichiro , Sugawara Yohei , Ohe Yuichiro , Hamamoto Ryuji
Published: July 2020

Recent studies have demonstrated the usefulness of convolutional neural networks (CNNs) to classify images of melanoma, with accuracies comparable to those achieved by dermatologists. However, the performance of a CNN trained with only clinical images of a pigmented skin lesion in a clinical image classification task, in competition with dermatologists, has not been reported to date. In this study, we extracted 5846 clinical images of pigmented skin lesions from 3551 patients. Pigmented skin lesions included malignant tumors (malignant melanoma and basal cell carcinoma) and benign tumors (nevus, seborrhoeic keratosis, senile lentigo, and hematoma/hemangioma). We created the test dataset by randomly selecting 666 patients out of them and picking one image per patient, and created the training dataset by giving bounding-box annotations to the rest of the images (4732 images, 2885 patients). Subsequently, we trained a faster, region-based CNN (FRCNN) with the training dataset and checked the performance of the model on the test dataset. In addition, ten board-certified dermatologists (BCDs) and ten dermatologic trainees (TRNs) took the same tests, and we compared their diagnostic accuracy with FRCNN. For six-class classification, the accuracy of FRCNN was 86.2%, and that of the BCDs and TRNs was 79.5% (p = 0.0081) and 75.1% (p < 0.00001), respectively. For two-class classification (benign or malignant), the accuracy, sensitivity, and specificity were 91.5%, 83.3%, and 94.5% by FRCNN; 86.6%, 86.3%, and 86.6% by BCD; and 85.3%, 83.5%, and 85.9% by TRN, respectively. False positive rates and positive predictive values were 5.5% and 84.7% by FRCNN, 13.4% and 70.5% by BCD, and 14.1% and 68.5% by TRN, respectively. We compared the classification performance of FRCNN with 20 dermatologists. As a result, the classification accuracy of FRCNN was better than that of the dermatologists. In the future, we plan to implement this system in society and have it used by the general public, in order to improve the prognosis of skin cancer.
Thiol Redox Regulation of Plant β-Carbonic Anhydrase
Authors: Dreyer Anna , Schackmann Alexander , Kriznik Alexandre , Chibani Kamel , Wesemann Corinna , Vogelsang Lara , Beyer André , Dietz Karl-Josef
Published: July 2020

β-carbonic anhydrases (βCA) accelerate the equilibrium formation between CO2 and carbonate. Two plant βCA isoforms are targeted to the chloroplast and represent abundant proteins in the range of >1% of chloroplast protein. While their function in gas exchange and photosynthesis is well-characterized in carbon concentrating mechanisms of cyanobacteria and plants with C4-photosynthesis, their function in plants with C3-photosynthesis is less clear. The presence of conserved and surface-exposed cysteinyl residues in the βCA-structure urged to the question whether βCA is subject to redox regulation. Activity measurements revealed reductive activation of βCA1, whereas oxidized βCA1 was inactive. Mutation of cysteinyl residues decreased βCA1 activity, in particular C280S, C167S, C230S, and C257S. High concentrations of dithiothreitol or low amounts of reduced thioredoxins (TRXs) activated oxidized βCA1. TRX-y1 and TRX-y2 most efficiently activated βCA1, followed by TRX-f1 and f2 and NADPH-dependent TRX reductase C (NTRC). High light irradiation did not enhance βCA activity in wildtype Arabidopsis, but surprisingly in βca1 knockout plants, indicating light-dependent regulation. The results assign a role of βCA within the thiol redox regulatory network of the chloroplast.
Structure-Antifouling Activity Relationship and Molecular Targets of Bio-Inspired(thio)xanthones
Authors: Almeida Joana R. , Palmeira Andreia , Campos Alexandre , Cunha Isabel , Freitas Micaela , Felpeto Aldo Barreiro , Turkina Maria V. , Vasconcelos Vitor , Pinto Madalena , Correia-da-Silva Marta , Sousa Emília
Published: July 2020

The development of alternative ecological and effective antifouling technologies is still challenging. Synthesis of nature-inspired compounds has been exploited, given the potential to assure commercial supplies of potential ecofriendly antifouling agents. In this direction, the antifouling activity of a series of nineteen synthetic small molecules, with chemical similarities with natural products, were exploited in this work. Six (4, 5, 7, 10, 15 and 17) of the tested xanthones showed in vivo activity toward the settlement of Mytilus galloprovincialis larvae (EC50: 3.53–28.60 µM) and low toxicity to this macrofouling species (LC50 > 500 µM and LC50/EC50: 17.42–141.64), and two of them (7 and 10) showed no general marine ecotoxicity (<10% of Artemia salina mortality) after 48 h of exposure. Regarding the mechanism of action in mussel larvae, the best performance compounds 4 and 5 might be acting by the inhibition of acetylcholinesterase activity (in vitro and in silico studies), while 7 and 10 showed specific targets (proteomic studies) directly related with the mussel adhesive structure (byssal threads), given by the alterations in the expression of Mytilus collagen proteins (PreCols) and proximal thread proteins (TMPs). A quantitative structure-activity relationship (QSAR) model was built with predictive capacity to enable speeding the design of new potential active compounds.
Better to Be Alone than in Bad Company: Cognate Synonyms Impair Word Learning
Authors: Antón Eneko , Duñabeitia Jon Andoni
Published: July 2020

The effects of cognate synonymy in L2 word learning are explored. Participants learned the names of well-known concrete concepts in a new fictional language following a picture-word association paradigm. Half of the concepts (set A) had two possible translations in the new language (i.e., both words were synonyms): one was a cognate in participants’ L1 and the other one was not. The other half of the concepts (set B) had only one possible translation in the new language, a non-cognate word. After learning the new words, participants’ memory was tested in a picture-word matching task and a translation recognition task. In line with previous findings, our results clearly indicate that cognates are much easier to learn, as we found that the cognate translation was remembered much better than both its non-cognate synonym and the non-cognate from set B. Our results also seem to suggest that non-cognates without cognate synonyms (set B) are better learned than non-cognates with cognate synonyms (set A). This suggests that, at early stages of L2 acquisition, learning a cognate would produce a poorer acquisition of its non-cognate synonym, as compared to a solely learned non-cognate. These results are discussed in the light of different theories and models of bilingual mental lexicon.
LncRNA DLG2-AS1 as a Novel Biomarker in Lung Adenocarcinoma
Authors: Arenas Alberto M. , Cuadros Marta , Andrades Alvaro , García Daniel J. , Coira Isabel F. , Rodríguez María Isabel , Baliñas-Gavira Carlos , Peinado Paola , Álvarez-Pérez Juan Carlos , Medina Pedro P.
Published: July 2020

Long non-coding RNAs (lncRNAs) are a heterogeneous class of non-coding RNAs whose biological roles are still poorly understood. LncRNAs serve as gene expression regulators, frequently interacting with epigenetic factors to shape the outcomes of crucial biological processes, and playing roles in different pathologies including cancer. Over the last years, growing scientific evidence supports the key role of some lncRNAs in tumor development and proposes them as valuable biomarkers for the clinic. In this study, we aimed to characterize lncRNAs whose expression is altered in tumor samples from patients with lung adenocarcinoma (LUAD) compared to adjacent normal tissue samples. On an RT-qPCR survey of 90 cancer-related lncRNAs, we found one lncRNA, DLG2-AS1, which was consistently downregulated in 70 LUAD patients. To gain insight into its biological function, DLG2-AS1 was cloned and successfully re-expressed in LUAD cancer cell lines. We determined that DLG2-AS1 is not a cis-regulatory element of its overlapping gene DLG2, as their transcription levels were not correlated, nor did DLG2-AS1 restoration modify the expression of DLG2 protein. Furthermore, after generating a receiver operating curve (ROC) and calculating the area under curve (AUC), we found that DLG2-AS1 expression showed high sensitivity and specificity (AUC = 0.726) for the classification of LUAD and normal samples, determining its value as a potential lung cancer biomarker.
Stromal CCL2 Signaling Promotes Mammary Tumor Fibrosis through Recruitment of Myeloid-Lineage Cells
Authors: Kuziel Genevra , Thompson Victoria , D’Amato Joseph V. , Arendt Lisa M.
Published: July 2020

Obesity is correlated with breast tumor desmoplasia, leading to diminished chemotherapy response and disease-free survival. Obesity causes chronic, macrophage-driven inflammation within breast tissue, initiated by chemokine ligand 2 (CCL2) signaling from adipose stromal cells. To understand how CCL2-induced inflammation alters breast tumor pathology, we transplanted oncogenically transformed human breast epithelial cells with breast stromal cells expressing CCL2 or empty vector into murine mammary glands and examined tumor formation and progression with time. As tumors developed, macrophages were rapidly recruited, followed by the emergence of cancer-associated fibroblasts (CAFs) and collagen deposition. Depletion of CD11b + myeloid lineage cells early in tumor formation reduced tumor growth, CAF numbers, and collagen deposition. CCL2 expression within developing tumors also enhanced recruitment of myeloid progenitor cells from the bone marrow into the tumor site. The myeloid progenitor cell population contained elevated numbers of fibrocytes, which exhibited platelet-derived growth factor receptor-alpha (PDGFRα)-dependent colony formation and growth in vitro. Together, these results suggest that chronic inflammation induced by CCL2 significantly enhances tumor growth and promotes the formation of a desmoplastic stroma through early recruitment of macrophages and fibrocytes into the tumor microenvironment. Fibrocytes may be a novel target in the tumor microenvironment to reduce tumor fibrosis and enhance treatment responses for obese breast cancer patients.
Plasmacytoid Dendritic Cell Impairment in Metastatic Melanoma by Lactic Acidosis
Authors: Monti Matilde , Vescovi Raffaella , Consoli Francesca , Farina Davide , Moratto Daniele , Berruti Alfredo , Specchia Claudia , Vermi William
Published: July 2020

The introduction of targeted therapies and immunotherapies has significantly improved the outcome of metastatic melanoma (MM) patients. These approaches rely on immune functions for their anti-melanoma response. Plasmacytoid dendritic cells (pDCs) exhibit anti-tumor function by production of effector molecules, type I interferons (I-IFNs), and cytokines. Tissue and blood pDCs result compromised in MM, although these findings are still partially conflicting. This study reports that blood pDCs were dramatically depleted in MM, particularly in patients with high lactate dehydrogenase (LDH) and high tumor burden; the reduced pDC frequency was associated with poor overall survival. Circulating pDCs resulted also in significant impairment in interferon alpha (IFN-α) and C-X-C motif chemokine 10 (CXCL10) production in response to toll-like receptor (TLR)-7/8 agonists; on the contrary, the response to TLR-9 agonist remained intact. In the BRAFV600+ subgroup, no recovery of pDC frequency could be obtained by BRAF and MEK inhibitors (BRAFi; MEKi), whereas their function was partially rescued. Mechanistically, in vitro exposure to lactic acidosis impaired both pDC viability and function. In conclusion, pDCs from MM patients were found to be severely impaired, with a potential role for lactic acidosis. Short-term responses to treatments were not associated with pDC recovery, suggesting long-lasting effects on their compartment.
The Perceived Impact of Length of the Diagnostic Pathway Is Associated with Health-Related Quality of Life of Sarcoma Survivors: Results from the Dutch Nationwide SURVSARC Study
Authors: Soomers Vicky L. M. N. , Desar Ingrid M. E. , van de Poll-Franse Lonneke V. , van de Sande Michiel A. J. van de , de Haan Jacco J. , Verhoef Cornelis , Vriens Ingeborg J. H. , van Houdt Winan J. , Bonenkamp Johannes J. , van der Graaf Winette T. A. van der , Husson Olga
Published: July 2020

Background: Sarcoma patients often experience a long time to diagnosis, known as the total interval. This interval can be divided into the patient (time from symptoms to doctor consultation) and diagnostic intervals (time from first consultation to diagnosis). In other cancers, a long total interval has been associated with worse outcomes, but its effect on health-related quality of life (HRQoL) has never been investigated among sarcoma patients. This study investigates the association between (1) the actual time to diagnosis and HRQoL; (2) the perceived impact of the diagnostic interval length and HRQoL; (3) the actual length and perceived impact of the length and the HRQoL of sarcoma survivors. Methods: A cross-sectional study was performed among sarcoma patients aged ≥18, diagnosed 2–10 years ago in the Netherlands. The participants completed a questionnaire on HRQoL, the time to diagnosis, the perceived impact of the diagnostic interval on HRQoL, and coping. Results: 1099 participants were included (response rate, 58%). The mean time since diagnosis was 67.4 months. More than half reported a patient (60%) or diagnostic interval (55%) ≥1 month. A third (31%) perceived a negative impact of the diagnostic interval length on HRQoL. Patient or diagnostic interval length was not associated with HRQoL. By contrast, participants perceiving a negative impact (32%) had lower HRQoL scores than those perceiving a positive (11%) or no impact (58%) (p = 0.000). This association remained significant in a multivariable model, in which maladaptive coping strategies and tumour characteristics were also found to be associated with HRQoL. Participants perceiving a negative impact of the length of the diagnostic interval related this to high psychological distress levels, more physical disabilities, and worse prognosis. Conclusion: The perceived impact of the diagnostic interval length was associated with the HRQoL of sarcoma survivors, whereas the actual length was not associated with HRQoL. Maladaptive coping strategies were independently associated with HRQoL. This offers opportunities for early intervention to improve HRQoL.
Wnt-5A/B Signaling in Hematopoiesis throughout Life
Authors: Mastelaro de Rezende Marina , Zenker Justo Giselle , Julian Paredes-Gamero Edgar , Gosens Reinoud
Published: July 2020

Wnt signaling is well-known to play major roles in the hematopoietic system, from embryogenesis to aging and disease. In addition to the main β-catenin-dependent pathway, it is now clear that Wnt5a and the structurally related Wnt5b are essential for hematopoiesis, bone marrow colonization and the final steps of hematopoietic stem cell (HSC) maturation via β-catenin-independent signaling. Wnt5a and Wnt5b ligands prevent hematopoietic exhaustion (by maintaining quiescent, long-term HSCs), induce the proliferation of progenitors, and guide myeloid development, in addition to being involved in the development of aging-related alterations. The aim of this review is to summarize the current knowledge on these roles of Wnt5a and Wn5b signaling in the hematopoietic field.
Multilayer-Structured Wood Electroless Cu–Ni Composite Coatings for Electromagnetic Interference Shielding
Authors: Pan Yanfei , Yin Dingwen , Yu Xiaofang , Hao Nanyi , Huang Jintian
Published: July 2020

The lightweight multilayer-structured electromagnetic interference shielding composite coatings with controllable electromagnetic gradient on wood surface were prepared via a simple multiple electroless copper–nickel (Cu–Ni) approach. The surface morphology, conductivity, hydrophobicity property and electromagnetic shielding effectiveness of the composite coatings were investigated. The surface roughness and conductivity of the composite coatings were enhanced with the increase in the number of depositions. The surface morphology demonstrated that the roughness was decreased with the process of multiple electroless. The coatings were compact and homogeneous as the deposition run was three. Here, the Sa (Sa illustrated Surface Roughness) value of coatings was 4.497 μm. The ideal conductivity of composite coatings can be obtained as the number of depositions was four. Electromagnetic shielding effectiveness reached average 90.69 dB in the frequency range from 300 kHz to 2.0 GHz. This study provides a new pathway for fabricating lightweight multilayer-structured electromagnetic interference shielding with controllable electromagnetic gradient and hydrophobic composite coatings-based wood.
Variable Angle Spectroscopic Ellipsometry Characterization of Reduced Graphene Oxide Stabilized with Poly(Sodium 4-Styrenesulfonate)
Authors: Politano Grazia Giuseppina , Vena Carlo , Desiderio Giovanni , Versace Carlo
Published: July 2020

Lately, the optical properties of Graphene Oxide (GO) and Reduced Graphene Oxide (RGO) films have been studied in the ultraviolet and visible spectral range. However, the accurate optical properties in the extended near-infrared and mid-infrared range have not been published yet. In this work, we report a Variable Angle Spectroscopic Ellipsometry (VASE) characterization of GO thin films dip-coated on SiO2/Si substrates and thermally reduced GO films in the 0.38–4.1 eV photon energy range. Moreover, the optical properties of RGO stabilized with poly(sodium 4-styrenesulfonate) (PSS) films dip-coated on SiO2/Si substrates are studied in the same range for the first time. The Lorentz optical models fit well with the experimental data. In addition, the morphological properties of the samples were investigated by Scanning Electron Microscopy (SEM) analysis.
Milling of Complex Surfaces of EN 10060 Steel after HVOF Sprayed NiCrBSi Coatings
Authors: Valíček Jan , Harničárová Marta , Řehoř Jan , Kušnerová Milena , Fulemová Jaroslava , Gombár Miroslav , Kučerová Ludmila , Filipenský Jan , Hnátík Jan
Published: July 2020

The high-velocity oxy-fuel spraying process was used to investigate and improve the surface properties of a workpiece. The research was focused on the spherical surface of a workpiece made of high-strength steel, a ball and socket assembly. After spraying with a nickel alloy, the surface was machined by milling. The coating was carried out as a process in which a very thin layer of coating of the required thickness and the required specific properties, i.e., high Vickers hardness, adhesion to the surface, wear resistance and other important characteristics, which must be respected in other machining methods, was applied to the already finished, heat-prepared metal substrate. This article deals with the milling of complex surfaces of steel substrate EN 10060 after spraying with NiCrBSi alloy. After spraying, a total of 15 milling experiments were performed in order to determine precisely the optimal cutting parameters of milling and surface adhesion, based on newly acquired prediction relations. The main presented results are new relations for the determination of optimal technological milling conditions based on the identification of adhesive sections using derived equations. The new relations were verified and also compared with the current literature in the field.
Effects of Micro-Arc Oxidation Process Parameters on Characteristics of Calcium-Phosphate Containing Oxide Layers on the Selective Laser Melted Ti13Zr13Nb Alloy
Authors: Dziaduszewska Magda , Shimabukuro Masaya , Seramak Tomasz , Zielinski Andrzej , Hanawa Takao
Published: July 2020

Titania-based films on selective laser melted Ti13Zr13Nb have been formed by micro-arc oxidation (MAO) at different process parameters (voltage, current, processing time) in order to evaluate the impact of MAO process parameters in calcium and phosphate (Ca + P) containing electrolyte on surface characteristic, early-stage bioactivity, nanomechanical properties, and adhesion between the oxide coatings and substrate. The surface topography, surface roughness, pore diameter, elemental composition, crystal structure, surface wettability, and the early stage-bioactivity in Hank’s solution were evaluated for all coatings. Hardness, maximum indent depth, Young’s modulus, and Ecoating/Esubstrate, H/E, H3/E2 ratios were determined in the case of nanomechanical evaluation while the MAO coating adhesion properties were estimated by the scratch test. The study indicated that the most important parameter of MAO process influencing the coating characteristic is voltage. Due to the good ratio of structural and nanomechanical properties of the coatings, the optimal conditions of MAO process were found at 300 V during 15 min, at 32 mA or 50 mA of current, which resulted in the predictable structure, high Ca/P ratio, high hydrophilicity, the highest demonstrated early-stage bioactivity, better nanomechanical properties, the elastic modulus and hardness well close to the values characteristic for bones, as compared to specimens treated at a lower voltage (200 V) and uncoated substrate, as well as a higher critical load of adhesion and total delamination.
Complex Analysis of Emission Properties of LEDs with 1D and 2D PhC Patterned by EBL
Authors: Suslik Lubos , Skriniarova Jaroslava , Kovac Jaroslav , Pudis Dusan , Kuzma Anton , Kovac Jaroslav
Published: July 2020

In this paper, we present the optical and electrical properties of surface-patterned GaAs-based Multiquantum Well (MQW) light emitting diodes (LEDs) with one- and two-dimensional photonic crystal (PhC) structures. Optical properties were analyzed in the near and far field, measured by a near-field scanning optical microscope and with a goniophotometer. We demonstrated a strong effect of patterned PhC on the radiation properties and the light extraction efficiency. Enormous surface emission enhancement reaching 110% confirmed the strong effect of the patterned structure on the coupling of the guided modes into the surface emission. Additionally, the considerable effect of the PhC structure diffraction on radiation pattern was confirmed in the near and far field and is in good agreement with the simulated shape of the optical field.