bluuebunny/crossref_metadata_2025 huggingface dataset:
Histone Modifying Enzymes in Gynaecological Cancers
Authors: Ramarao-Milne Priya , Kondrashova Olga , Barry Sinead , Hooper John D. , Lee Jason S. , Waddell Nicola
Published: February 2021

Genetic and epigenetic factors contribute to the development of cancer. Epigenetic dysregulation is common in gynaecological cancers and includes altered methylation at CpG islands in gene promoter regions, global demethylation that leads to genome instability and histone modifications. Histones are a major determinant of chromosomal conformation and stability, and unlike DNA methylation, which is generally associated with gene silencing, are amenable to post-translational modifications that induce facultative chromatin regions, or condensed transcriptionally silent regions that decondense resulting in global alteration of gene expression. In comparison, other components, crucial to the manipulation of chromatin dynamics, such as histone modifying enzymes, are not as well-studied. Inhibitors targeting DNA modifying enzymes, particularly histone modifying enzymes represent a potential cancer treatment. Due to the ability of epigenetic therapies to target multiple pathways simultaneously, tumours with complex mutational landscapes affected by multiple driver mutations may be most amenable to this type of inhibitor. Interrogation of the actionable landscape of different gynaecological cancer types has revealed that some patients have biomarkers which indicate potential sensitivity to epigenetic inhibitors. In this review we describe the role of epigenetics in gynaecological cancers and highlight how it may exploited for treatment.
A Palette of Cytokines to Measure Anti-Tumor Efficacy of T Cell-Based Therapeutics
Authors: Ramesh Prathyaya , Shivde Rohan , Jaishankar Dinesh , Saleiro Diana , Le Poole I. Caroline
Published: February 2021

Cytokines are key molecules within the tumor microenvironment (TME) that can be used as biomarkers to predict the magnitude of anti-tumor immune responses. During immune monitoring, it has been customary to predict outcomes based on the abundance of a single cytokine, in particular IFN-γ or TGF-β, as a readout of ongoing anti-cancer immunity. However, individual cytokines within the TME can exhibit dual opposing roles. For example, both IFN-γ and TGF-β have been associated with pro- and anti-tumor functions. Moreover, cytokines originating from different cellular sources influence the crosstalk between CD4+ and CD8+ T cells, while the array of cytokines expressed by T cells is also instrumental in defining the mechanisms of action and efficacy of treatments. Thus, it becomes increasingly clear that a reliable readout of ongoing immunity within the TME will have to include more than the measurement of a single cytokine. This review focuses on defining a panel of cytokines that could help to reliably predict and analyze the outcomes of T cell-based anti-tumor therapies.
Intracellular Autofluorescence as a New Biomarker for Cancer Stem Cells in Glioblastoma
Authors: Vieira de Castro Joana , S. Gonçalves Céline , P. Martins Eduarda , Miranda-Lorenzo Irene , T. Cerqueira Mariana , Longatto-Filho Adhemar , A. Pinto Afonso , L. Reis Rui , Sousa Nuno , Heeschen Christopher , M. Costa Bruno
Published: February 2021

The identification of cancer stem cells (CSCs), which are implicated in tumor initiation, progression, therapy resistance, and relapse, is of great biological and clinical relevance. In glioblastoma (GBM), this is still a challenge, as no single marker is able to universally identify populations of GBM cancer stem cells (GSCs). Indeed, there is still controversy on whether biomarker-expressing cells fulfill the functional criteria of bona fide GSCs, despite being widely used. Here, we describe a novel subpopulation of autofluorescent (Fluo+) cells in GBM that bear all the functional characteristics of GSCs, including higher capacity to grow as neurospheres, long-term self-renewal ability, increased expression of stem cell markers, and enhanced in vivo tumorigenicity. Mechanistically, the autofluorescent phenotype is largely due to the intracellular accumulation of riboflavin, mediated by the ABC transporter ABCG2. In summary, our work identifies an intrinsic cellular autofluorescent phenotype enriched in GBM cells with functional stem cells features that can be used as a novel, simple and reliable biomarker to target these highly malignant tumors, with implications for GBM biological and clinical research.
MicroRNA-27b-3p Targets the Myostatin Gene to Regulate Myoblast Proliferation and Is Involved in Myoblast Differentiation
Authors: Zhang Genxi , He Mingliang , Wu Pengfei , Zhang Xinchao , Zhou Kaizhi , Li Tingting , Zhang Tao , Xie Kaizhou , Dai Guojun , Wang Jinyu
Published: February 2021

microRNAs play an important role in the growth and development of chicken embryos, including the regulation of skeletal muscle genesis, myoblast proliferation, differentiation, and apoptosis. Our previous RNA-seq studies showed that microRNA-27b-3p (miR-27b-3p) might play an important role in regulating the proliferation and differentiation of chicken primary myoblasts (CPMs). However, the mechanism of miR-27b-3p regulating the proliferation and differentiation of CPMs is still unclear. In this study, the results showed that miR-27b-3p significantly promoted the proliferation of CPMs and inhibited the differentiation of CPMs. Then, myostatin (MSTN) was confirmed to be the target gene of miR-27b-3p by double luciferase reporter assay, RT-qPCR, and Western blot. By overexpressing and interfering with MSTN expression in CPMs, the results showed that overexpression of MSTN significantly inhibited the proliferation and differentiation of CPMs. In contrast, interference of MSTN expression had the opposite effect. This study showed that miR-27b-3p could promote the proliferation of CPMs by targeting MSTN. Interestingly, both miR-27b-3p and MSTN can inhibit the differentiation of CPMs. These results provide a theoretical basis for further understanding the function of miR-27b-3p in chicken and revealing its regulation mechanism on chicken muscle growth.
Heterogeneity of Intestinal Tissue Eosinophils: Potential Considerations for Next-Generation Eosinophil-Targeting Strategies
Authors: Masterson Joanne C. , Menard-Katcher Calies , Larsen Leigha D. , Furuta Glenn T. , Spencer Lisa A.
Published: February 2021

Eosinophils are implicated in the pathophysiology of a spectrum of eosinophil-associated diseases, including gastrointestinal eosinophilic diseases (EGIDs). Biologics that target the IL-5 pathway and are intended to ablate eosinophils have proved beneficial in severe eosinophilic asthma and may offer promise in treating some endotypes of EGIDs. However, destructive effector functions of eosinophils are only one side of the coin; eosinophils also play important roles in immune and tissue homeostasis. A growing body of data suggest tissue eosinophils represent a plastic and heterogeneous population of functional sub-phenotypes, shaped by environmental (systemic and local) pressures, which may differentially impact disease outcomes. This may be particularly relevant to the GI tract, wherein the highest density of eosinophils reside in the steady state, resident immune cells are exposed to an especially broad range of external and internal environmental pressures, and greater eosinophil longevity may uniquely enrich for co-expression of eosinophil sub-phenotypes. Here we review the growing evidence for functional sub-phenotypes of intestinal tissue eosinophils, with emphasis on the multifactorial pressures that shape and diversify eosinophil identity and potential targets to inform next-generation eosinophil-targeting strategies designed to restrain inflammatory eosinophil functions while sustaining homeostatic roles.
Primary Signet Ring Cell/Histiocytoid Carcinoma of the Eyelid: Somatic Mutations in CDH1 and Other Clinically Actionable Mutations Imply Early Use of Targeted Agents
Authors: Wang Lei-Chi , Lin Tai-Chi , Yeh Yi-Chen , Ho Hsiang-Ling , Tsai Chieh-Chih , Chou Teh-Ying
Published: February 2021

Primary signet ring cell/histiocytoid carcinoma of the eyelid is a rare ocular malignancy and its diagnosis is often delayed. This neoplasm presents as an insidious, diffusely infiltrative mass in the periocular area that later infiltrates the orbit. An exenteration is usually indicated; however, nearly one-third of patients develop local recurrence or metastasis. Morphologically, it resembles signet ring cell carcinoma of the stomach and breast, raising the possibility of mutations in CDH1, the gene encoding E-cadherin. To determine whether primary signet ring cell/histiocytoid carcinoma harbors the CDH1 mutation or other actionable mutations, we analyzed the tumor tissue via next-generation sequencing. We identified only one case of primary signet ring cell carcinoma of the eyelid with adequate DNA quality for sequencing from the pathological archive during the period 2000 to 2020. A comprehensive evaluation including histopathology, immunohistochemistry, and next-generation sequencing assay was performed on tumor tissue. Immunohistochemically, the tumor exhibited E-cadherin membranous staining with the aberrant cytoplasmic staining of β-catenin. Using next-generation sequencing, we demonstrated the mutation in the CDH1 gene. In addition, other clinically actionable mutations including ERBB2 and PIK3CA were also detected. The alterations in other actionable genes indicate a need for larger studies to evaluate the pathogenesis and potential therapies for primary signet ring cell/histiocytoid carcinoma of the eyelid.
Dataset of Two-Dimensional Gel Electrophoresis Images of Acute Myeloid Leukemia Patients before and after Induction Therapy
Authors: Urrea Juan E. , Restrepo Luisa F. , Prada-Arismendy Jeanette , Castillo Erwing , Goez Manuel M. , Torres-Madronero Maria C. , Delgado-Trejos Edilson , Röthlisberger Sarah
Published: February 2021

Acute myeloid leukemia (AML) is a malignant disorder of the hematopoietic stem and progenitor cells, which results in the build-up of immature blasts in the bone marrow and eventually in the peripheral blood of affected patients. Accurately assessing a patient´s prognosis is very important for clinical management of the disease, which is why there are several prognostic factors such as age, performance status at diagnosis, platelet count, serum creatinine and albumin that are taken into account by the clinician when deciding the course of treatment. However, proteomic changes related to treatment response in this patient group have not been widely explored. Here, we make available a set of 22 two-dimensional gel electrophoresis (2DGE) images obtained from the peripheral blood samples of 11 patients with AML, taken at the time of diagnosis and after induction therapy (approximately 21–28 days after starting treatment). The same set of 2DGE images is also made available after a preprocessing stage (an additional 22 2DGE pre-processed images), which was performed using algorithms developed in Python, in order to improve the visualization of characteristic spots and facilitate proteomic analysis of this type of images.
Information Rate in Humans during Visuomotor Tracking
Authors: Lam Sze-Ying , Zénon Alexandre
Published: February 2021

Previous investigations concluded that the human brain’s information processing rate remains fundamentally constant, irrespective of task demands. However, their conclusion rested in analyses of simple discrete-choice tasks. The present contribution recasts the question of human information rate within the context of visuomotor tasks, which provides a more ecologically relevant arena, albeit a more complex one. We argue that, while predictable aspects of inputs can be encoded virtually free of charge, real-time information transfer should be identified with the processing of surprises. We formalise this intuition by deriving from first principles a decomposition of the total information shared by inputs and outputs into a feedforward, predictive component and a feedback, error-correcting component. We find that the information measured by the feedback component, a proxy for the brain’s information processing rate, scales with the difficulty of the task at hand, in agreement with cost-benefit models of cognitive effort.
Information Theory: Deep Ideas, Wide Perspectives, and Various Applications
Authors: Ben-Gal Irad , Kagan Evgeny
Published: February 2021

The history of information theory, as a mathematical principle for analyzing data transmission and information communication, was formalized in 1948 with the publication of Claude Shannon’s famous paper “A Mathematical Theory of Communication” [...]
Development of a Two-Stage DQFM to Improve Efficiency of Single- and Multi-Hazard Risk Quantification for Nuclear Facilities
Authors: Choi Eujeong , Kwag Shinyoung , Ha Jeong-Gon , Hahm Daegi
Published: February 2021

The probabilistic safety assessment (PSA) of a nuclear power plant (NPP) under single and multiple hazards is one of the most important tasks for disaster risk management of nuclear facilities. To date, various approaches—including the direct quantification of the fault tree using the Monte Carlo simulation (DQFM) method—have been employed to quantify single- and multi-hazard risks to nuclear facilities. The major advantage of the DQFM method is its applicability to a partially correlated system. Other methods can represent only an independent or a fully correlated system, but DQFM can quantify the risk of partially correlated system components by the sampling process. However, as a sampling-based approach, DQFM involves computational costs which increase as the size of the system and the number of hazards increase. Therefore, to improve the computational efficiency of the conventional DQFM, a two-stage DQFM method is proposed in this paper. By assigning enough samples to each hazard point according to its contribution to the final risk, the proposed two-stage DQFM can effectively reduce computational costs for both single- and multi-hazard risk quantification. Using examples of single- and multi-hazard threats to nuclear facilities, the effectiveness of the proposed two-stage DQFM is successfully demonstrated. Especially, two-stage DQFM saves computation time of conventional DQFM up to 72% for multi-hazard example.
Determination of Zero Dimensional, Apparent Devolatilization Kinetics for Biomass Particles at Suspension Firing Conditions
Authors: Espekvist Anna , Li Tian , Glarborg Peter , Løvås Terese , Jensen Peter Arendt
Published: February 2021

As part of the strive for a carbon neutral energy production, biomass combustion has been widely implemented in retrofitted coal burners. Modeling aids substantially in prediction of biomass flame behavior and thus in boiler chamber conditions. In this work, a simple model for devolatilization of biomass at conditions relevant for suspension firing is presented. It employs Arrhenius parameters in a single first order (SFOR) devolatilization reaction, where the effects of kinetics and heat transfer limitations are lumped together. In this way, a biomass particle can be modeled as a zero dimensional, isothermal particle, facilitating computational fluid dynamic calculations of boiler chambers. The zero dimensional model includes the effects of particle aspect ratio, particle density, maximum gas temperature, and particle radius. It is developed using the multivariate data analysis method, partial least squares regression, and is validated against a more rigorous semi-2D devolatilization model. The model has the capability to predict devolatilization time for conditions in the parameter ranges; radius (39–1569 μμm), density (700–1300 kg/m3), gas temperature (1300–1900 K), aspect ratio (1.01–8). Results show that the particle radius and gas phase temperature have a large influence on the devolatilization rate, and the aspect ratio has a comparatively smaller effect, which, however, cannot be neglected. The impact of aspect ratio levels off as it increases. The model is suitable for use as stand alone or as a submodel for biomass particle devolatilization in CFD models.
Zero-Sequence Voltage Injection Method for DC Capacitor Voltage Balancing of Wye-Connected CHB Converter under Unbalanced Grid and Load Conditions
Authors: Kim Jae-Myeong , Song Geum-Seop , Jung Jae-Jung
Published: February 2021

Leg capacitor energy balancing control is one of the crucial issues for stable operation of a cascaded H-bridge (CHB) converter. Because this topology inherently consists of numerous submodule cells with DC capacitors, the cell voltages and leg capacitor energy instantaneously fluctuate depending on operation sequence of the CHB converter. In general, a wye-connected CHB-converter-based static synchronous compensator (STATCOM) utilizes a zero-sequence voltage component for leg capacitor energy balancing. In this paper, to improve the dynamics of leg energy balancing control, a feedforward calculation method of the zero-sequence voltage injection is proposed. The feedforward term can be instantaneously calculated by using the information from the measured leg voltages and leg currents, and the method ensures successful regulation of the leg energy balance even under unbalanced grid and load conditions. Moreover, the verification of the proposed method is supported by the mathematical vector theorems. A 50MVA full-scale wye-connected CHB–STATCOM system simulation was performed to verify the proposed feedforward calculation method considering unbalanced grid as well as unbalanced load conditions.
Modeling Water Droplet Freezing and Collision with a Solid Surface
Authors: Shayunusov Doston , Eskin Dmitry , Balakin Boris V. , Chugunov Svyatoslav , Johansen Stein Tore , Akhatov Iskander
Published: February 2021

Water droplets released from the sea surface represent one of the major causes of ice accretion on marine vessels. A one-dimensional model of the freezing of a spherical water droplet moving in cold air was developed. The crystallization model allows one to obtain an analytical solution if a uniform temperature distribution over the liquid’s core is assumed. The model was validated using STAR CCM+ Computational fluid dynamics (CFD) code. A collision of a partially frozen droplet with a solid wall assuming the plastic deformation of an ice crust was also considered. The ratio of the crust deformation to the crust thickness was evaluated. It was assumed that if this ratio were to exceed unity, the droplet would stick to the wall’s surface due to ice bridge formation caused by the water released from the droplet’s core.
Model-Based Design of a Pseudo-Cogenerative Heating System for e-Boat Battery Cold Start
Authors: Fusai Dario , Soldati Alessandro , Lusignani Davide , Santarelli Paolo , Patroncini Paolo
Published: February 2021

Full-electric boats are an expression of recent advancements in the area of vessel electrification. The installed batteries can suffer from poor cold-start performance, especially in the frigid season and at higher latitudes, leading to driving power limitations immediately after startup. At state, the leading solution is to adopt a dedicated heater placed on the common cooling/heating circuit; this implies poor volume, weight, and cost figures, given the very limited duty cycle of such a part. The Heater-in-Converter (HiC) technology allows removing this specialized component, exploiting the power electronics converters already available on board: HiC modulates their efficiency to produce valuable heat (pseudo-cogeneration). In this work, we use the model-based approach to design this system, which requires heating power minimization to fulfill power electronics limitations, while guaranteeing the user-expected startup time to full power. A multistage model is used to get the yearly vessel temperature distribution from latitude information and some additional data. Then, a lumped parameter for the cooling/heating circuit is used to determine the minimum required power as a function of the properties of the thermal interface material used for the battery coupling. The design is validated on a 1:5 test bench (battery power and energy), which demonstrates how the technology can be to scaled up to also fit different boats and battery sizes.
Active Power Filter Pre-Selection Tool to Enhance the Power Quality in Oil and Gas Platforms
Authors: Vitoi Lais Abrantes , Brandao Danilo , Tedeschi Elisabetta
Published: February 2021

This paper proposes a preliminary design tool for active power filters’ (APFs) solutions to be applied in offshore oil and gas platforms, where power quality indices are typically low, and reactive power compensation and current harmonic mitigation are often desired. The proposed approach considers that APF selection and rating is a trade-off between performance and size, and that both component and system aspects need to be optimized to achieve a well-tailored solution. As size and weight are critical constraints in offshore applications, possible benefits of using Silicon Carbide (SiC) switches for the APF implementation are investigated. Moreover, different compensation strategies are compared, varying the connection point of the APF between two different voltage levels and assigning the APFs different compensation goals. Improvements in power quality indices, as well as APFs rating, number of components, power losses, and filter size, have been considered for both SiC and Silicon-based solutions to identify the best trade-offs suitable for the considered, energy intensive industrial application.
Investigation of Environmental Leaching Behavior of an Innovative Method for Landfilling of Waste Incineration Air Pollution Control Residues
Authors: Wächter Mihail Reinhold , Ionel Ioana , Dan Daniel , Negrea Adina
Published: February 2021

Waste incineration air pollution control (APC) residues require pretreatment before landfilling because these types of residues encompass pollutants from an incineration gas stream. The environmental concerns of APC residues consist of a risk of leaching and subsequent release of potentially harmful substances that occur under environmental exposure. The stabilization/solidification (S/S) method of incineration residues is one of the most applied technologies for hazardous incineration residues. Portland cement is commonly used as a binder material in S/S for pollutant encapsulation, in order to change the hydrological characteristics of the landfilled material. Based on previous research, an innovative S/S method for APC residues is investigated, meant to replace Portland cement with cement-like material made from lignite fly ash (FA). To do this, a lab-scale landfill was created through the promoted S/S method and exposed to the environment for 12 months. Thus, this article assesses the lab-scale leaching behavior of a landfill disposal material exposed to environmental conditions and attempts to prove the promoted innovative S/S method. The results show that the replacement of Portland cement with a substitute material for utilization in the S/S method can mitigate energy consumption in the industrial cement subsector.
Improving GIS-Based Heat Demand Modelling and Mapping for Residential Buildings with Census Data Sets at Regional and Sub-Regional Scales
Authors: Schwanebeck Malte , Krüger Marcus , Duttmann Rainer
Published: February 2021

Heat demand of buildings and related CO2 emissions caused by energy supply contribute to global climate change. Spatial data-based heat planning enables municipalities to reorganize local heating sectors towards efficient use of regional renewable energy resources. Here, annual heat demand of residential buildings is modeled and mapped for a German federal state to provide regional basic data. Using a 3D building stock model and standard values of building-type-specific heat demand from a regional building typology in a Geographic Information Systems (GIS)-based bottom-up approach, a first base reference is modeled. Two spatial data sets with information on the construction period of residential buildings, aggregated on municipality sections and hectare grid cells, are used to show how census-based spatial data sets can enhance the approach. Partial results from all three models are validated against reported regional data on heat demand as well as against gas consumption of a municipality. All three models overestimate reported heat demand on regional levels by 16% to 19%, but underestimate demand by up to 8% on city levels. Using the hectare grid cells data set leads to best prediction accuracy values at municipality section level, showing the benefit of integrating this high detailed spatial data set on building age.
Assessment of a District Trigeneration Biomass Powered Double Organic Rankine Cycle as Primed Mover and Supported Cooling
Authors: Tauseef Nasir Muhammad , Ekwonu Michael Chukwuemeka , Park Yoonseong , Abolfazli Esfahani Javad , Kim Kyung Chun
Published: February 2021

This study presents a combined cooling, heating, and power system powered by biogas, suitable for small scale communities in remote locations. To run such a system, in order to obtain the daily life essentials of electricity, hot water, and cooling, municipal waste can be considered as an option. Furthermore, the organic Rankine cycle part of the organic Rankine cycle powered vapor compression chiller can be used in times of need for additional electric production. The system comprises a medium temperature organic Rankine cycle utilizing M-xylene as its working fluid, and the cooling was covered by an Isobutane vapor compression cycle powered by an R245fa employing organic Rankine cycle. The system analyzed was designated to provide 250 kW of electricity. The energetic and exergetic analysis was performed, considering several system design parameters. The impact of the design parameters in the prime mover has a much greater effect on the whole system. The system proposed can deliver cooling values at the rate between 9.19 and 22 kW and heating values ranging from 879 up to 1255 kW, depending on the design parameter. Furthermore, the second law efficiency of the system was found to be approximately 56% at the baseline conditions and can be increased to 64.5%.
Efficient, Load Independent and Self-Regulated Wireless Power Transfer with Multiple Loads for Long Distance IoT Applications
Authors: Hassan Najam ul , Lee Woochan , Lee Byunghun
Published: February 2021

This paper proposes a wireless power transfer (WPT) system by placing repeater coils to transfer power to multiple loads for the internet of things (IoT) devices and sensors in smart grid applications. The proposed system intermediate resonators (repeaters) not only function as power relays to enhance the transfer distance but also supplies power to its load attached to them. Equal power distribution and load-independent characteristics were obtained without efficiency degradation when any one of the loads was changed during system operation. Identical high-quality factor coils were designed using Litz-wire to reduce the skin effect. The coil size was 15.5 cm × 15.5 cm and the four relays achieved total efficiency of 51.7%, delivering 2 W power and output voltage of 5 V to each load with a total power transfer distance of 62 cm.
Investigations on the Performance of Various Horizontal Ground Electrodes
Authors: Hizamul-Din Hanis Hamizah , Nor Normiza Mohamad , Ahmad Nurul Nadia , Idris Nur Farahi , Mahmud Azwan
Published: February 2021

Experimental work on grounding systems has shown that the characteristics of grounding systems under high impulse conditions are different than those obtained at steady-state conditions. Investigations on the grounding systems under high impulse conditions make it evident that ionization process could occur in soil, due to field enhancement in air voids in the soil. This process can lead to an ionization zone that reduces the impulse resistance of grounding systems (due to an enlargement of a virtually increased cross-sectional area) from its steady state. There have been many studies pointing towards the effect of various soils and the ground electrode’s arrangement on the reduction of impulse resistance from its steady state, and its decrease with increasing currents. It was, however, noted that very few studies on the effect of the configurations of horizontal ground electrodes have been performed by field measurements before. This work presents the experimental and simulated work of various configurations of ground electrodes, with spikes which are thought to enhance the ionization process in soil. In this paper, field measurement was set up, and the results of field measurements were applied with finite element method (FEM) to obtain the electric field values. It was demonstrated that with the addition of spike on the electrodes, a high electric field was computed. The time to peak current, discharged time and impulse impedance were also analyzed for various horizontal ground electrodes.
Central and Eastern European CO2 Market—Challenges of Emissions Trading for Energy Companies
Authors: Ciesielska-Maciągowska Dorota , Klimczak Dawid , Skrzek-Lubasińska Małgorzata
Published: February 2021

The aim of this article was to identify challenges of emissions trading that the Polish and CEE Central and Eastern Europe energy industry will face, as well as to indicate key implications for the competitiveness of the companies from the energy sector resulting from that trading. The EU Emissions Trading Scheme (ETS) is the emissions trading system, which results from the EU policy concerning climate change. It is a tool for reducing greenhouse gas emissions (GHG). The system regulates an annual allocation of the allowances. The price of CO2 emission allowances is subject to constant fluctuations because it depends on various macroeconomic factors as well as is an effect of proprietary trading by global investment banks. Polish energy companies have an increasing share in the emission of CO2 in the European market. This is due to the fact that other European countries are rapidly moving away from fossil fuel-fired sources. The cost per MWh related to CO2 price has been growing in the last 10 years from ca. 5 up to 30 EUR/MWh at the beginning of 2021. From an electric power utilities perspective, the ability to set up a proper strategy in trading CO2 will be crucial to be competitive in the wholesale power market. The higher price of CO2 (and electric power) at the domestic market in relation to more green (more renewable energy sources RES in energy mix) surrounding countries translates into a worse competitive position.
Effects of the COVID-19 Pandemic on Energy Systems and Electric Power Grids—A Review of the Challenges Ahead
Authors: Navon Aviad , Machlev Ram , Carmon David , Onile Abiodun Emmanuel , Belikov Juri , Levron Yoash
Published: February 2021

The COVID-19 pandemic represents not just a global health crisis, but may signal the beginning of a new era of economic activity, the potential consequences of which we currently do not fully understand. In this context, the mid-to-long-range impacts of the pandemic on the energy sector have been studied extensively in the last few months. Despite these efforts, the pandemic still raises many open questions concerning the long-term operation and planning of power systems. For instance, how will the pandemic affect the integration of renewable energy sources? Should current power system expansion plans change in light of the COVID-19 pandemic? What new tools should be provided to support system operators during global health crises? It is the purpose of this paper to better understand the many aspects of these open questions by reviewing the relevant recent literature and by analyzing measured data. We point out the main challenges that the pandemic introduced by presenting patterns of electricity generation and demand, frequency deviations, and load forecasting. Moreover, we suggest directions for future research that may assist in coping with the mentioned challenges. We hope that this paper will trigger fruitful discussions and encourage further research on these important emerging topics.
Fast Calculation of Cube and Inverse Cube Roots Using a Magic Constant and Its Implementation on Microcontrollers
Authors: Moroz Leonid , Samotyy Volodymyr , Walczyk Cezary J. , Cieśliński Jan L.
Published: February 2021

We develop a bit manipulation technique for single precision floating point numbers which leads to new algorithms for fast computation of the cube root and inverse cube root. It uses the modified iterative Newton–Raphson method (the first order of convergence) and Householder method (the second order of convergence) to increase the accuracy of the results. The proposed algorithms demonstrate high efficiency and reduce error several times in the first iteration in comparison with known algorithms. After two iterations 22.84 correct bits were obtained for single precision. Experimental tests showed that our novel algorithm is faster and more accurate than library functions for microcontrollers.
First-Decade Biomass and Carbon Accumulation, and Woody Community Change after Severe Wind Damage in a Hemlock-White Pine Forest Remnant
Authors: Peterson Chris
Published: February 2021

Studies of biomass and carbon dynamics and community composition change after forest wind disturbance have predominantly examined trends after low and intermediate severity events, while studies after very severe wind disturbance have been few. This study documents trends in aboveground biomass and carbon across 10 years of forest recovery after severe wind disturbance. In July 1989, a tornado struck mature Tsugacanadensis-Pinusstrobus forest in northwest Connecticut, USA, causing damage across roughly 8 ha. Canopy tree damage and regeneration were surveyed in 1991 and 1999. Two primary hypotheses were tested, both of which derive from regeneration being primarily via the release of suppressed saplings rather than new seedling establishment: (1) Biomass and carbon accumulation will be faster than accumulation reported from a similar forest that experienced similar severity of wind disturbance; and (2) The stand will experience very little change in species composition or diversity. Estimated immediate post-disturbance (1989) aboveground live-tree carbon was 20.7 ± 43.9 Mg ha−1 (9.9% of pre-disturbance) Ten years after the disturbance, carbon in aboveground live tree biomass increased to 37.1 ± 47.9 Mg ha−1; thus for the first decade, annual accumulation averaged 1.6 Mg ha−1 of carbon; this was significantly faster than the rate reported in a similar forest that experienced similar severity of wind disturbance. The species diversity of woody stems ten years after the disturbance was significantly higher (nonoverlapping confidence intervals of rarefaction curves) than pre-disturbance canopy trees. Thus, hypothesis 1 was confirmed while hypothesis 2 was rejected. This study augments the limited number of longer-term empirical studies that report biomass and carbon accumulation rates after wind disturbance, and can therefore serve as a benchmark for mechanistic and modeling research.
A Sign of Things to Come: Predicting the Perception of Above-the-Fold Time in Web Browsing
Authors: Jahromi Hamed Z. , Delaney Declan , Hines Andrew
Published: February 2021

Content is a key influencing factor in Web Quality of Experience (QoE) estimation. A web user’s satisfaction can be influenced by how long it takes to render and visualize the visible parts of the web page in the browser. This is referred to as the Above-the-fold (ATF) time. SpeedIndex (SI) has been widely used to estimate perceived web page loading speed of ATF content and a proxy metric for Web QoE estimation. Web application developers have been actively introducing innovative interactive features, such as animated and multimedia content, aiming to capture the users’ attention and improve the functionality and utility of the web applications. However, the literature shows that, for the websites with animated content, the estimated ATF time using the state-of-the-art metrics may not accurately match completed ATF time as perceived by users. This study introduces a new metric, Plausibly Complete Time (PCT), that estimates ATF time for a user’s perception of websites with and without animations. PCT can be integrated with SI and web QoE models. The accuracy of the proposed metric is evaluated based on two publicly available datasets. The proposed metric holds a high positive Spearman’s correlation (rs=0.89) with the Perceived ATF reported by the users for websites with and without animated content. This study demonstrates that using PCT as a KPI in QoE estimation models can improve the robustness of QoE estimation in comparison to using the state-of-the-art ATF time metric. Furthermore, experimental result showed that the estimation of SI using PCT improves the robustness of SI for websites with animated content. The PCT estimation allows web application designers to identify where poor design has significantly increased ATF time and refactor their implementation before it impacts end-user experience.
Evaluating the Influence of a G-Quadruplex Prone Sequence on the Transactivation Potential by Wild-Type and/or Mutant P53 Family Proteins through a Yeast-Based Functional Assay
Authors: Monti Paola , Brazda Vaclav , Bohálová Natália , Porubiaková Otília , Menichini Paola , Speciale Andrea , Bocciardi Renata , Inga Alberto , Fronza Gilberto
Published: February 2021

P53, P63, and P73 proteins belong to the P53 family of transcription factors, sharing a common gene organization that, from the P1 and P2 promoters, produces two groups of mRNAs encoding proteins with different N-terminal regions; moreover, alternative splicing events at C-terminus further contribute to the generation of multiple isoforms. P53 family proteins can influence a plethora of cellular pathways mainly through the direct binding to specific DNA sequences known as response elements (REs), and the transactivation of the corresponding target genes. However, the transcriptional activation by P53 family members can be regulated at multiple levels, including the DNA topology at responsive promoters. Here, by using a yeast-based functional assay, we evaluated the influence that a G-quadruplex (G4) prone sequence adjacent to the p53 RE derived from the apoptotic PUMA target gene can exert on the transactivation potential of full-length and N-terminal truncated P53 family α isoforms (wild-type and mutant). Our results show that the presence of a G4 prone sequence upstream or downstream of the P53 RE leads to significant changes in the relative activity of P53 family proteins, emphasizing the potential role of structural DNA features as modifiers of P53 family functions at target promoter sites.
Participation of TFIIIB Subunit Brf1 in Transcription Regulation in the Human Pathogen Leishmania major
Authors: Florencio-Martínez Luis E. , Cano-Santiago Andrés , Mondragón-Rosas Fabiola , Gómez-García Maricarmen , Flores-Pérez Carlos , Román-Carraro Fiordaliso C. , Barocio-Rodríguez Luis A. , Manning-Cela Rebeca G. , Nepomuceno-Mejía Tomás , Martínez-Calvillo Santiago
Published: February 2021

In yeast and higher eukaryotes, transcription factor TFIIIB is required for accurate initiation of transcription by RNA Polymerase III (Pol III), which synthesizes transfer RNAs (tRNAs), 5S ribosomal RNA (rRNA), and other essential RNA molecules. TFIIIB is composed of three subunits: B double prime 1 (Bdp1), TATA-binding protein (TBP), and TFIIB-related factor 1 (Brf1). Here, we report the molecular characterization of Brf1 in Leishmania major (LmBrf1), a parasitic protozoan that shows distinctive transcription characteristics, including the apparent absence of Pol III general transcription factors TFIIIA and TFIIIC. Although single-knockout parasites of LmBrf1 were obtained, attempts to generate LmBrf1-null mutants were unsuccessful, which suggests that LmBrf1 is essential in promastigotes of L. major. Notably, Northern blot analyses showed that the half-lives of the messenger RNAs (mRNAs) from LmBrf1 and other components of the Pol III transcription machinery (Bdp1 and Pol III subunit RPC1) are very similar (~40 min). Stabilization of these transcripts was observed in stationary-phase parasites. Chromatin immunoprecipitation (ChIP) experiments showed that LmBrf1 binds to tRNA, small nuclear RNA (snRNA), and 5S rRNA genes. Unexpectedly, the results also indicated that LmBrf1 associates to the promoter region of the 18S rRNA genes and to three Pol II-dependent regions here analyzed. Tandem affinity purification and mass spectrometry analyses allowed the identification of a putative TFIIIC subunit. Moreover, several proteins involved in transcription by all three RNA polymerases co-purified with the tagged version of LmBrf1.
Estimating Copy-Number Proportions: The Comeback of Sanger Sequencing
Authors: Seroussi Eyal
Published: February 2021

Determination of the relative copy numbers of mixed molecular species in nucleic acid samples is often the objective of biological experiments, including Single-Nucleotide Polymorphism (SNP), indel and gene copy-number characterization, and quantification of CRISPR-Cas9 base editing, cytosine methylation, and RNA editing. Standard dye-terminator chromatograms are a widely accessible, cost-effective information source from which copy-number proportions can be inferred. However, the rate of incorporation of dye terminators is dependent on the dye type, the adjacent sequence string, and the secondary structure of the sequenced strand. These variable rates complicate inferences and have driven scientists to resort to complex and costly quantification methods. Because these complex methods introduce their own biases, researchers are rethinking whether rectifying distortions in sequencing trace files and using direct sequencing for quantification will enable comparable accurate assessment. Indeed, recent developments in software tools (e.g., TIDE, ICE, EditR, BEEP and BEAT) indicate that quantification based on direct Sanger sequencing is gaining in scientific acceptance. This commentary reviews the common obstacles in quantification and the latest insights and developments relevant to estimating copy-number proportions based on direct Sanger sequencing, concluding that bidirectional sequencing and sophisticated base calling are the keys to identifying and avoiding sequence distortions.
The Uncertainty of COVID-19 Inducing Social Fear and Pressure on the Continuity of Rural, Community-Based Medical Education: A Thematic Analysis
Authors: Ohta Ryuichi , Ryu Yoshinori , Sano Chiaki
Published: February 2021

Rural community-based medical education (CBME) enriches undergraduate and postgraduate students’ learning but has been impacted by the coronavirus disease 2019 (COVID-19) pandemic. We identified the challenges faced by stakeholders as well as the relevant solutions to provide recommendations for sustainable CBME in community hospitals during the COVID-19 pandemic. A total of 31 pages of field and reflection notes were collated through direct observation and used for analysis. Five physicians, eight nurses, one clerk, fourteen medical trainees, and three rural citizens were interviewed between 1 April and 30 September 2020. The interviews were recorded and their contents were transcribed verbatim and analyzed using thematic analysis. Three themes emerged: uncertainty surrounding COVID-19, an overwhelming sense of social fear and pressure within and outside communities, and motivation and determination to continue providing CBME. Rural CBME was impacted by not only the fear of infection but also social fear and pressure within and outside communities. Constant assessment of the risks associated with the pandemic and the implications for CMBE is essential to ensure the sustainability of CBME in rural settings, not only for medical educators and students but also stakeholders who administrate rural CBME.
Engaging Mothers on the Growth of School-Age Children in a Rural South African Health and Demographic Site: A Qualitative Insight
Authors: Modjadji Perpetua
Published: February 2021

A qualitative study was conducted to explore mothers’ insights on the growth of school-age children in a rural Health and Demographic site of Limpopo Province, in South Africa. The participants were selected using purposive sampling. Data were collected from seven focus group discussions, which were audio-taped and transcribed verbatim. NVivo10 was used to analyse interview transcripts, following qualitative thematic analysis. Fifty-four mothers aged between 27 and 52 years were interviewed. Unfavourable sociodemographic status with poor living conditions of mothers were observed, particularly in terms of unemployment, minimal tertiary education, and rural locality. The perceptions of mothers on child growth linked growth of their children to various factors such as poverty and socioeconomic status, genetic/family heredity, and household environment. Mothers further related child growth to purchasing power and decisions regarding types of food, food unavailability, affordability issues, feeding beliefs and practices; and child food preferences, school feeding schemes, and maternal and societal cultural beliefs and practices. Despite their concerns, mothers perceived that their children were growing well, but differently. It is worth noting that the views of mothers on child growth were up to their aptitude level and might have been restricted due to their level of education and rural locality. Hence, there is a need for novel information, education, and communication strategies to effectively reach mothers, especially in rural areas, regarding the importance of identifying children with growth failure and its prevention. Mothers should be able to identify when a child is affected by growth failure and to seek healthcare, in order to prevent children from progressing to severe forms. This study informs on the timing of nutritional interventions for children and context-specific health promotion and health education programs to improve the knowledge of mothers on child growth.
Characteristics of Confidence and Preparedness in Paramedics in Metropolitan, Regional, and Rural Australia to Manage Mental-Health-Related Presentations: A Cross-Sectional Study
Authors: Emond Kate , Bish Melanie , Savic Michael , Lubman Dan I. , McCann Terence , Smith Karen , Mnatzaganian George
Published: February 2021

Mental-health-related presentations account for a considerable proportion of the paramedic’s workload in prehospital care. This cross-sectional study aimed to examine the perceived confidence and preparedness of paramedics in Australian metropolitan and rural areas to manage mental-health-related presentations. Overall, 1140 paramedics were surveyed. Pearson chi-square and Fisher exact tests were used to compare categorical variables by sex and location of practice; continuous variables were compared using the non-parametric Mann–Whitney and Kruskal–Wallis tests. Perceived confidence and preparedness were each modelled in multivariable ordinal regressions. Female paramedics were younger with higher qualifications but were less experienced than their male counterparts. Compared to paramedics working in metropolitan regions, those working in rural and regional areas were generally older with fewer qualifications and were significantly less confident and less prepared to manage mental health presentations (p = 0.001). Compared to male paramedics, females were less confident (p = 0.003), although equally prepared (p = 0.1) to manage mental health presentations. These results suggest that higher qualifications from the tertiary sector may not be adequately preparing paramedics to manage mental health presentations, which signifies a disparity between education provided and workforce preparedness. Further work is required to address the education and training requirements of paramedics in regional and rural areas to increase confidence and preparedness in managing mental health presentations.