bluuebunny/crossref_metadata_2025 huggingface dataset:
Early Signs of the Effects of Forest Fragmentation on the Genetic Diversity and Structure of the Threatened Ecuadorian Tree Ocotea rotundata (Lauraceae)
Authors: Marques Isabel , Draper David , Riofrío Lorena , Naranjo Carlos
Published: November 2022

The diversity of genetic resources is essential to cope with environmental changes. However, despite forests play a crucial role in mitigating changes, genetic knowledge has scarcely been used for forest conservation. In this study, we used nuclear microsatellites to understand the patterns of genetic diversity and population genetic structure in Ocotea rotundata van der Werff (Lauraceae), an endemic Ecuadorian tree, highly affected by habitat changes and fragmentation. Our results show high levels of genetic diversity, except in one population. The level of genetic differentiation between populations was low and genetic clusters showed no apparent spatial pattern. In fact, a high degree of genetic admixture was found between most populations. Migration rates were asymmetric but overall high, except in one population, where outgoing gene dispersal was limited. Nevertheless, allelic fixation values suggested a general deficit in heterozygotes, probably due to an increase in the levels of mating between close relatives. Although long-lived organisms, such as trees, can often accumulate a surprising amount of genetic diversity, the results found here could be an early sign of a decline in the diversity of O. rotundata. These findings provide baseline information on genetic resources to support future restoration programs to mitigate the impacts of changes in O. rotundata populations.
The Impact of Integrated Harvesting Systems on Productivity, Costs, and Amount of Logging Residue in the Clear-Cutting of a Larix kaempferi (Lamb.) Carr. Stand
Authors: Woo Heesung , Lee Eunjai , Acuna Mauricio , Cho Hyunmin , Han Sang-Kyun
Published: November 2022

Two integrated harvesting methods have been primarily applied to increase the opportunity for forest biomass utilization. In Korea, small shovels with a carrier for cut-to-length harvesting (CTL system) and tower yarders for whole-tree harvesting (WT system) are commonly used for the transportation of tree assortments (i.e., sawlogs and logging residue). No previous studies are available in South Korea that have compared and highlighted the operational performance and yield of logging residues between the CTL and WT harvesting systems. Thus, our study’s main objectives were to (1) evaluate the productivity and costs of the two harvesting systems through a standard time study method and (2) estimate the amount of harvesting logging residue at the landing. The productivities of the CTL and WT systems were 1.45 and 2.99 oven-dried tons (odt)/productive machine hour (PMH), at a cost of 86.81 and 45.41 USD/odt, respectively. In the WT system, the amount of logging residue (2.1 odt/ha) collected at the landing was approximately four-times larger than that of the CTL system (0.5 odt/ha). Our results suggested that the WT system is a less expensive and more suitable system when there are markets demanding logs and biomass, whereas the CTL system remains a less expensive option for stem-only harvesting. Furthermore, these results are important for estimating the economic and environmental amount of residue that could be potentially recovered and utilized from the forest types included in the study.
Further Mining and Characterization of miRNA Resource in Chinese Fir (Cunninghamia lanceolata)
Authors: Deng Houyin , Huang Rong , Hu Dehuo , Wang Runhui , Wei Ruping , Yan Su , Wu Guandi , Sun Yuhan , Li Yun , Zheng Huiquan
Published: November 2022

In this study, we aimed to expand the current miRNA data bank of Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.) regarding its potential value for further genetic and genomic use in this species. High-throughput small RNA sequencing successfully captured 140 miRNAs from a Chinese fir selfing family harboring vigor and depressed progeny. Strikingly, 75.7% (n = 106) of these miRNAs have not been documented previously, and most (n = 105) of them belong to the novel set with 6858 putative target genes. The new datasets were then integrated with the previous information to gain insight into miRNA genetic architecture in Chinese fir. Collectively, a relatively high proportion (62%, n = 110) of novel miRNAs were found. Furthermore, we identified one MIR536 family that has not been previously documented in this species and four overlapped miRNA families (MIR159, MIR164, MIR171_1, and MIR396) from new datasets. Regarding the stability, we calculated the secondary structure free energy and found a relatively low R2 value (R2 < 0.22) between low minimal folding free energy (MFE) of pre-miRNAs and MFE of its corresponding mature miRNAs in most datasets. When in view of the conservation aspect, the phylogenetic trees showed that MIR536 and MIR159 sequences were highly conserved in gymnosperms.
Association of Polymorphisms in the Long Non-Coding RNA HOTAIR with Recurrent Pregnancy Loss in a Korean Population
Authors: Park Hyeon Woo , Kim Young Ran , Lee Jeong Yong , Ko Eun Ju , Kwon Min Jung , Kim Ji Hyang , Kim Nam Keun
Published: November 2022

Recurrent pregnancy loss (RPL) affects 1% to 5% of women, with devastating effects on both reproductive health and psychological well-being. Homeobox (HOX) transcript antisense RNA (HOTAIR) is a long non-coding RNA (lncRNA) produced by HOXC; it plays a major role in invasion and development of ovarian and other cancers. The aim of the present study was to analyze effects of HOTAIR polymorphisms (rs4759314 A>G, rs920778 T>C, rs1899663 G>T, and rs7958904 G>C) on RPL in Korean women. A total of 403 women with RPL and 383 healthy women were selected for this study. Genotyping analysis was performed with the polymerase chain reaction, restriction fragment length polymorphism, and the TaqMan genotyping assay. Clinical characteristics were compared using Student’s unpaired t-test and the chi-square test for categorical variables. Logistic regression was performed to evaluate associations between single nucleotide polymorphisms and RPL incidence. In all assays, p < 0.05 was considered significant. HOTAIR polymorphisms rs4759314A>G and rs920778T>C were highly associated with increased risk of RPL, specifically the haplotypes rs4759314A>G/rs1899663G>T (G-T) and rs4759314A>G/rs920778 T>C (G-C). These associations were maintained in haplotypes that contained three polymorphisms (rs4759314 A>G, rs920778 T>C, and rs1899663 G>T) A-C-G, G-T-G, and G-T-T, further indicating that the HOTAIR rs4759314 and rs920778 polymorphisms play significant roles in idiopathic RPL in Korean women.
Pituitary-Gland-Based Genes Participates in Intrauterine Growth Restriction in Piglets
Authors: Ji Xiang , Shen Qi , Wu Pingxian , Chen Hongyue , Wang Shujie , Chen Dong , Yu Yang , Guo Zongyi , Wang Jinyong , Tang Guoqing
Published: November 2022

Intrauterine growth restriction (IUGR) is a major problem associated with piglet growth performance. The incidence of IUGR is widespread in Rongchang pigs. The pituitary gland is important for regulating growth and metabolism, and research has identified genes associated with growth and development. The pituitary gland of newborn piglets with normal birth weight (NBW group, n = 3) and (IUGR group, n = 3) was collected for transcriptome analysis. A total of 323 differentially expression genes (DEGs) were identified (|log2(fold-change)| > 1 and q value < 0.05), of which 223 were upregulated and 100 were downregulated. Gene Ontology (GO) functional and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses showed that the DEGs were mainly related to the extracellular matrix, regulation of the multicellular organismal process, tissue development and angiogenesis, which participate in the growth and immune response in IUGR piglets. Moreover, 7 DEGs including IGF2, THBS1, ITGA1, ITGA8, EPSTI1, FOSB, and UCP2 were associated with growth and immune response. Furthermore, based on the interaction network analysis of the DEGs, two genes, IGF2 and THBS1, participated in cell proliferation, embryonic development and angiogenesis. IGF2 and THBS1 were also the main genes participating in the IUGR. This study identified the core genes involved in IUGR in piglets and provided a reference for exploring the effect of the pituitary gland on piglet growth.
Multi-Omics Nutritional Approaches Targeting Metabolic-Associated Fatty Liver Disease
Authors: Ramos-Lopez Omar
Published: November 2022

Currently, metabolic-associated fatty liver disease (MAFLD) is a leading global cause of chronic liver disease, and is expected to become one of the most common indications of liver transplantation. MAFLD is associated with obesity, involving multiple mechanisms such as alterations in lipid metabolism, insulin resistance, hyperinflammation, mitochondrial dysfunction, cell apoptosis, oxidative stress, and extracellular matrix formation. However, the onset and progression of MAFLD is variable among individuals, being influenced by intrinsic (personal) and external environmental factors. In this context, sequence structural variants across the human genome, epigenetic phenomena (i.e., DNA methylation, histone modifications, and long non-coding RNAs) affecting gene expression, gut microbiota dysbiosis, and metabolomics/lipidomic fingerprints may account for differences in MAFLD outcomes through interactions with nutritional features. This knowledge may contribute to gaining a deeper understanding of the molecular and physiological processes underlying MAFLD pathogenesis and phenotype heterogeneity, as well as facilitating the identification of biomarkers of disease progression and therapeutic targets for the implementation of tailored nutritional strategies. This comprehensive literature review highlights the potential of nutrigenetic, nutriepigenetic, nutrimetagenomic, nutritranscriptomics, and nutrimetabolomic approaches for the prevention and management of MAFLD in humans through the lens of precision nutrition.
Revealing the Regulatory Mechanism of lncRNA-LMEP on Melanin Deposition Based on High-Throughput Sequencing in Xichuan Chicken Skin
Authors: Zhang Pengwei , Cao Yanfang , Fu Yawei , Zhu Huiyuan , Xu Shuohui , Zhang Yanhua , Li Wenting , Sun Guirong , Jiang Ruirui , Han Ruili , Li Hong , Li Guoxi , Tian Yadong , Liu Xiaojun , Kang Xiangtao , Li Donghua
Published: November 2022

The therapeutic, medicinal, and nourishing properties of black-bone chickens are highly regarded by consumers in China. However, some birds may have yellow skin (YS) or light skin rather than black skin (BS), which causes economic losses every year. Long noncoding RNAs (lncRNAs) are widely present in living organisms, and they perform various biological functions. Many genes associated with BS pigmentation have been discovered, but the lncRNAs involved and their detailed mechanisms have remained untested. We detected 56 differentially expressed lncRNAs from the RNA-seq of dorsal skin (BS versus YS) and found that TCONS_00054154 plays a vital role in melanogenesis by the combined analysis of lncRNAs and mRNAs. We found that the full length of the TCONS_00054154 sequence was 3093 bp by RACE PCR, and we named it LMEP. Moreover, a subcellular localization analysis identified that LMEP is mainly present in the cytoplasm. After the overexpression and the interference with LMEP, the tyrosinase content significantly increased and decreased, respectively (p < 0.05). In summary, we identified the important lncRNAs of chicken skin pigmentation and initially determined the effect of LMEP on melanin deposition.
“Digital Dividend” or “Digital Divide”: What Role Does the Internet Play in the Health Inequalities among Chinese Residents?
Authors: Zhang Dongling , Zhang Guoqing , Jiao Yuxin , Wang Yanyan , Wang Pengnian
Published: November 2022

With the vigorous development of the medical industry in China, residents’ health has been significantly improved. However, along with the income gap, urban–rural gap, and healthcare resource gap caused by economic development, health inequality has become a fundamental barrier to the promotion of residents’ health. The popularity of the Internet has helped close the gap to some extent, but it also has drawbacks. Using data from the China Family Panel Studies (CFPS) from 2014 to 2018, we evaluated the effects of Internet usage on health disparities among residents using fixed effect models, mediation effect models, and other methodologies. The findings indicate that Internet usage can help to minimize health inequality since it lowers income inequality, promotes health consciousness, and reduces depression. Furthermore, Internet usage plays a greater role on the health improvement of the middle-aged, the elderly, urban residents, and females. Although the Internet has brought “digital dividends” in general, the Internet usage rates among different groups also reveal that there is a clear “digital gap” among rural residents, elderly groups, and low-income groups. These results have significant implications for promoting healthcare equality.
Characteristics of Cancers in Community Members Exposed to the World Trade Center Disaster at a Young Age
Authors: Florsheim Rebecca Lynn , Zhang Qiao , Durmus Nedim , Zhang Yian , Pehlivan Sultan , Arslan Alan A. , Shao Yongzhao , Reibman Joan
Published: November 2022

The destruction of the World Trade Center (WTC) towers on 11 September 2001 (9/11) released tons of dust and smoke into the atmosphere, exposing hundreds of thousands of community members (survivors) and responders to carcinogens. The WTC Environmental Health Center (WTC EHC) is a federally designated surveillance and treatment program for community members who were present in the New York City disaster area on 9/11 or during the months that followed. WTC EHC enrollment requires exposure to the WTC dust and fumes and a federally certifiable medical condition, which includes most solid and blood cancers. Several studies have described the prevalence and characteristics of cancers in responders and survivors exposed to the WTC dust and fumes as adults. Cancers in those exposed at a young age warrant specific investigation since environmental toxin exposure at a younger age may change cancer risk. We describe the characteristics of 269 cancer patients with 278 cancer diagnoses among WTC EHC enrollees who were young in age (aged 0 to 30) on 9/11. These include 215 patients with a solid tumor (79.9%) and 54 with a lymphoid and/or hematopoietic cancer (20.1%). Among them, 9 patients had a known second primary cancer. A total of 23 different types of cancer were identified, including cancer types rare for this age group. Many were diagnosed in individuals lacking traditional cancer-specific risk factors such as tobacco use. The current study is the first to report specifically on cancer characteristics of younger enrollees in the WTC EHC program.
Health Communication and Inequalities in Primary Care Access during the COVID-19 Pandemic among Ethnic Minorities in the United Kingdom: Lived Experiences and Recommendations
Authors: Ekezie Winifred , Maxwell Akilah , Byron Margaret , Czyznikowska Barbara , Osman Idil , Moylan Katie , Gong Sarah , Pareek Manish
Published: November 2022

Health Communication is critical in the context of public health and this was highlighted during the COVID-19 pandemic. Ethnic minority groups were significantly impacted during the pandemic; however, communication and information available to them were reported to be insufficient. This study explored the health information communication amongst ethnic communities in relation to their experiences with primary health care services during the COVID-19 pandemic. The research used qualitative methodology using focus groups and semi-structured interviews with community members and leaders from three ethnic minority communities (African-Caribbean, Somali and South Asian) in Leicester, United Kingdom. The interviews were audio recorded, transcribed, and open-coded. Rigour was determined through methodological coherence, appropriate and sufficient sampling, and iterative data collection and analysis. Six focus groups and interviews were conducted with 42 participants. Four overarching themes were identified related to health communication, experiences, services and community recommendations to improve primary care communication. To address primary care inequalities effectively and improve future health communication strategies, experiences from the pandemic should be reflected upon, and positive initiatives infused into the healthcare strategies, especially for ethnic minority communities.
The Emergence of Extracellular Electron Mediating Functionality in Rice Straw-Artificial Soil Mixture during Humification
Authors: Hu Tingting , Pham Duyen Minh , Kasai Takuya , Katayama Arata
Published: November 2022

This study aimed to elucidate the origin of extracellular electron mediating (EEM) functionality and redox-active center(s) in humic substances, where they are ubiquitously distributed. Here, we show the emergence of EEM functionality during the humification of rice straw in artificial soil (kaolin and sand) with a matric potential of −100 cm at 20 °C for one year. We used the dechlorination activity of an EEM material-dependent pentachlorophenol-dechlorinating anaerobic microbial consortium as an index of the EEM functionality. Although rice straw and its mixture with artificial soil did not initially have EEM functionality, it emerged after one month of humification and increased until six months after which the functionality was maintained for one year. Chemical and electrochemical characterizations demonstrated that the emergence and increase in EEM functionality were correlated with the degradation of rice straw, formation of quinone structures, a decrease in aromatic structures, an increase in nitrogenous and aliphatic structures, and specific electric capacitance during humification. The newly formed quinone structure was suggested as a potential redox-active center for the EEM functionality. These findings provide novel insights into the dynamic changes in EEM functionality during the humification of organic materials.
The Effectiveness of Culturally Specific Male Domestic Violence Offender Intervention Programs on Behavior Changes and Mental Health: A Systematic Review
Authors: Satyen Lata , Hansen Ashlyn , Green Jane Louise , Zark Laura
Published: November 2022

The objective of domestic violence intervention programs is to address perpetrator behavior. However, the suitability and effectiveness of these programs in confronting problematic behavior for ethnically diverse groups is unclear. Therefore, the aim of this systematic review was to cohesively examine whether such programs are effective in reducing recidivism, changing perpetrator behavior, and addressing mental health issues for culturally diverse groups. Several databases were searched for peer-reviewed articles that included culturally specific components or ethnically diverse cohorts in offender intervention programs. 10 articles met the inclusion criteria. The findings demonstrate greater effectiveness of programs with greater cultural engagement: through culturally trained facilitators, addressing the cultural and patriarchal norms relevant to the specific client group, and discussion of gender roles and attitudes to gender equality specific to the cultural context. Such programs achieved some positive outcomes including: reduced recidivism, improved mental health, and better attitudes to gender equality. However, the findings are limited to a few ethnically diverse groups, and not all studies measured all outcomes listed above. This review suggests the development and implementation of suitable offender intervention programs that address perpetrator behavior and mental health in ethnically diverse client groups. When culturally relevant and effective programs are implemented, it could lead to men’s modification of perpetrating behavior and create safer family relationships.
Maxillofacial Injuries Sustained by Riders of Electric-Powered Bikes and Electric-Powered Scooters
Authors: Arbel Shimrit , Zrifin Elad , Mahmoud Reema , Mijiritsky Eitan , Groysman Leonid , Shuster Amir , Rahima Heled , Kleinman Shlomi , Ianculovici Clariel , Peleg Oren
Published: November 2022

Objectives: The purpose of our study is to retrospectively analyze and compare the patterns of maxillofacial-related injuries among rides of electric-powered bikes (E-bikes) and electric-powered scooters (E-scooters), the associated risk factors, and the required treatment. Materials and methods: The medical files of all riders presenting to the emergency department at the Tel Aviv Sourasky Medical Center between 2019 and 2020 with oral- and maxillofacial-related injuries due to E-bike and E-scooter accidents were reviewed. Results: A total of 320 riders sustained oral- and maxillofacial-related injuries due to trauma involving E-bikes and E-scooters during the study period. E-scooter riders were involved in 238 accidents (74.5%) while E-bike riders accounted for the remaining 82 accidents (27.5%). Eighty-four out of 320 riders (26.25%) were hospitalized and required surgical interventions. Most of the 232 riders (72.5%) who reported not wearing a protective helmet during the index accident were E-scooter riders. In addition, 39 riders (18.66%) were riding either of these electric-powered vehicles under the influence of alcohol. Conclusions: E-bike riders are more likely to sustain a maxillofacial fracture than E-scooter riders. Not wearing a protective helmet and riding under the influence of alcohol are major risk factors for maxillofacial injuries.
Association between Workplace Violence and Depressive Symptoms among Primary Healthcare Professionals in Shandong, China: Meaning in Life as a Moderator
Authors: Wang Meiqi , Wang Haipeng , Wei Zhen , Wang Yifan , Sun Long
Published: November 2022

Background: Workplace violence (WPV) is common in healthcare settings. However, the association between WPV and depressive symptoms has not been explored among primary healthcare professionals, especially in China. The assumption of the moderating effort of meaning in life on the association has also not been tested. The purposes of the study are to investigate the relationship between WPV and depressive symptoms and identify the moderating role of meaning in life among primary healthcare professionals in China. Methods: In this study, we collected 2530 valid questionnaires. The participants were composed of primary healthcare professionals in Shandong province, China. WPV, meaning in life (including presence of life and search for life), depressive symptoms, and some social-demographic variables were evaluated. The SPSS macro was used to build the moderating relationship. Results: WPV was positively correlated with depressive symptoms (β = 9.09, p < 0.001), and meaning in life was negatively associated with WPV and depressive symptoms (β = −0.13, p < 0.05) among primary healthcare professionals in China. For primary healthcare professionals with low presence of life, presence of life aggravated the relationship. Conclusions: The current study has verified the association between WPV and meaning in life, and the relationship between WPV and depressive symptoms has been supported among primary healthcare professionals in China. Meaning in life and presence of life as moderators play crucial roles in the relationship between WPV and depressive symptoms.
Decision Analysis of Manufacturer-Led Closed-Loop Supply Chain Considering Corporate Social Responsibility
Authors: Zhang Qi , Liu Yong , Liu Zhiyang
Published: November 2022

With the rapid development of the economy, a growing number of consumers and enterprises are paying attention to corporate social responsibility (CSR). Meanwhile, there exist a variety of conflicts in closed-loop supply chain management. To analyse and deal with the decision problems of the manufacturer-led closed-loop supply chain with CSR, by using the manufacturer Stackelberg game, we construct some basic models considering CSR, and exploit them to analyse the optimal decisions of supply chains with and without CSR under centralized and decentralized decision making and explore the influence of CSR on supply chain, and then we establish a coordination mechanism through two-part tariff.
Spatio-Temporal Variation and Its Driving Forces of Soil Organic Carbon along an Urban–Rural Gradient: A Case Study of Beijing
Authors: Liu Bingrui , Qian Jiacheng , Zhao Ran , Yang Qijun , Wu Kening , Zhao Huafu , Feng Zhe , Dong Jianhui
Published: November 2022

Rapid urbanization has reshaped land cover and the ecological environment, potentially improving or deteriorating soil organic carbon (SOC). However, the response of SOC to urbanization has not yet been fully exploited. Herein, by using the land-use transfer matrix, the Sen & Mann–Kendall tests, the Hurst index, and a geographical and temporal weighted regression (GTWR) model, as well as an urban–rural gradient perspective, we assessed the dynamic response of SOC to Beijing’s urbanization from 2001 to2015 and identified the main drivers. The results found that SOC stock decreased by 7651.50 t C during the study period. SOC density varied significantly along an urban–rural gradient, with high value areas mainly being located in remote mountainous rural areas and low value areas mainly being located in urban areas on the plains. There was an uneven variation in SOC density across the urban–rural gradient, with suburban areas (25–40 km away from urban cores) losing the most SOC density while urban areas and rural areas remained relatively unchanged. GTWR model revealed the spatio-temporal non-flat stability of various driving forces. Precipitation, the proportion of forest, the proportion of grassland, the population, distance to the urban center, the slope, and the silt content are the main factors related to SOC stock change. As a result, we suggest policy makers reconceptualize the uneven variation in the SOC between urban and rural areas, emphasize suburban areas as a target for controlling SOC loss, and take into consideration the spatial and temporal heterogeneity of the factors influencing SOC stock when evaluating policies.
The Potential for Managed Alcohol Programmes in Scotland during the COVID-19 Pandemic: A Qualitative Exploration of Key Areas for Implementation Using the Consolidated Framework for Implementation Research
Authors: Carver Hannah , Parkes Tessa , Masterton Wendy , Booth Hazel , Ball Lee , Murdoch Helen , Falzon Danilo , Pauly Bernie M.
Published: November 2022

People experiencing homelessness and alcohol dependence are at increased risk of a range of harms, including from COVID-19. Managed Alcohol Programmes (MAPs) are an alcohol harm reduction intervention specifically for this group. In this paper we report on qualitative findings of a mixed methods study investigating the potential utility of MAPs during the COVID-19 pandemic in Scotland. Interviews, conducted with 40 participants, explored potential views of implementing MAPs during the pandemic. Theoretically, we drew on the Consolidated Framework for Implementation Research (CFIR) to inform data collection and analysis. Six themes were identified which mapped onto three CFIR domains: perceptions of MAPs and the evidence base; necessary components of MAPs; changing culture of alcohol harm reduction; MAPs as a moral and ethical grey area; addressing a service gap; and securing buy-in and partnership working. Participants were generally positive about MAPs and viewed them as a key intervention to address a service gap. Several necessary components were identified for successful implementation of MAPs. Securing buy-in from a range of stakeholders and partnership working were deemed important. Finally, MAPs require careful, long-term planning before implementation. We conclude that MAPs are needed in Scotland and require long-term funding and appropriate resources to ensure they are successful.
Physicians’ Perceptions of and Barriers to Cardiopulmonary Rehabilitation for Heart Failure Patients in Saudi Arabia: A Cross-Sectional Study
Authors: Aldhahir Abdulelah M. , Alhotye Munyra , Alqahtani Jaber S. , Alghamdi Saeed M. , Alsulayyim Abdullah S. , Alqarni Abdullah A. , Alzahrani Eidan M. , Siraj Rayan A. , Alwafi Hassan
Published: November 2022

Background: Cardiopulmonary rehabilitation (CR) serves as a core component of the management strategy for patients with heart failure (HF). CR is administered by multidisciplinary healthcare providers, but their perceptions toward delivering CR to HF patients, and the factors and barriers that might influence referral, have not been studied. This study aims to assess physicians’ perceptions toward delivering CR programs to HF patients and identify factors and barriers that might influence their referral decisions. Methods: Between 15 February and 5 June 2022, a cross-sectional online survey with ten multiple-choice items was distributed to all general and cardiac physicians in Saudi Arabia. The characteristics of the respondents were described using descriptive statistics. Percentages and frequencies were used to report categorical variables. The statistical significance of the difference between categorical variables was determined using the chi-square (2) test. Logistic regression was used to identify referral factors. Results: Overall, 513 physicians (general physicians (78%) and cardiac doctors (22%)) completed the online survey, of which 65.0% (n = 332) were male. Of the general physicians, 236 (59%) had referred patients with HF to CR. Sixty-six (58%) of the cardiac doctors had referred patients with HF to CR. A hospital-supervised program was the preferred mode of delivering CR programs among 315 (79%) general physicians, while 84 (74%) cardiac doctors preferred to deliver CR programs at home. Apart from the exercise component, information about HF disease was perceived by 321 (80%) general physicians as the essential component of a CR program, while symptom management was perceived by 108 (95%) cardiac doctors as the essential component of a CR program. The most common patient-related factor that strongly influenced referral decisions was “fatigue related to disease” (63.40%). The availability of CR centers (48%) was the most common barrier preventing the referral of patients to CR. Conclusions: CR is an effective management strategy for HF patients, but the lack of CR centers is a major barrier to the referral of patients. A hospital-supervised program is the preferred method of delivering CR from the general physicians’ perspective, while cardiac doctors prefer home-based CR programs. Apart from the exercise component, information about HF disease and symptom management is essential components of CR programs from general physicians’ and cardiac doctors’ perspectives, respectively.
Relationships among Sports Group Cohesion, Passion, and Mental Toughness in Chinese Team Sports Athletes
Authors: Gu Song , Bi Sheng , Guan Zhixun , Fang Xuemo , Jiang Xulu
Published: November 2022

Background: Passion is an important motivational variable that profoundly affects athletes’ cognition, emotion, and behavior. This study constructed a mediating model to explore the mechanism of cohesion toward passion and mental toughness of Chinese team sports athletes and to investigate the mediating effect of harmonious passion and obsessive passion on cohesion and mental toughness. Methods: A questionnaire survey was conducted on 326 Chinese active athletes (M = 19.63, SD = 6.51) aged 14–26 years (54% male, 46% female) from eight sports. Results: Cohesion and its dimensions can positively predict athletes’ mental toughness, and ATG-T is more important in advantage analysis. The direct and indirect paths show that cohesion affects mental toughness through the mediating effect of harmonious passion and obsessive passion. Mediating effect model has a good fit and explained 22.1% of the variance in mental toughness. Conclusion: The relationship between cohesion, passion, and mental toughness reflects the psychological dynamic process from environment to motivation to sports performance. The development of team sports athletes’ mental toughness can be carried out from the following points. First, the team should define a sports goal and take the needs of members into account in goal-setting. Second, the sports team should build a team culture that is enterprising, inclusive, and cooperative and emphasizes members’ recognition of them. Third, the team should attach importance to the passion of the members and make good use of the team atmosphere. To protect the psychological health and long-term development of athletes, team culture should pay more attention to the cultivation of athletes’ harmonious passion. Improving cohesion is beneficial to athletes’ mental toughness in team sports. To protect the psychological health and long-term development of athletes, team culture should pay more attention to the cultivation of athletes’ harmonious passion.
Identification of Potential Artefacts in In Vitro Measurement of Vanadium-Induced Reactive Oxygen Species (ROS) Production
Authors: Zwolak Iwona , Wnuk Ewa , Świeca Michał
Published: November 2022

We investigated vanadium, i.e., a redox-active heavy metal widely known for the generation of oxidative stress in cultured mammalian cells, to determine its ability to interfere with common oxidative stress-related bioassays in cell-free conditions. We first assessed the prooxidant abilities (H2O2 level, oxidation of DHR 123, and DCFH-DA dyes) and antioxidant capacity (ABTS, RP, OH, and DPPH methods) of popular mammalian cell culture media, i.e., Minimal Essential Medium (MEM), Dulbecco’s Minimal Essential Medium (DMEM), Dulbecco’s Minimal Essential Medium-F12 (DMEM/F12), and RPMI 1640. Out of the four media studied, DMEM has the highest prooxidant and antioxidant properties, which is associated with the highest concentration of prooxidant and antioxidant nutrients in its formulation. The studied vanadium compounds, vanadyl sulphate (VOSO4), or sodium metavanadate (NaVO3) (100, 500, and 1000 µM), either slightly increased or decreased the level of H2O2 in the studied culture media. However, these changes were in the range of a few micromoles, and they should rather not interfere with the cytotoxic effect of vanadium on cells. However, the tested vanadium compounds significantly stimulated the oxidation of DCFH-DA and DHR123 in a cell-independent manner. The type of the culture media and their pro-oxidant and antioxidant abilities did not affect the intensity of oxidation of these dyes by vanadium, whereas the vanadium compound type was important, as VOSO4 stimulated DCFH-DA and DHR oxidation much more potently than NaVO3. Such interactions of vanadium with these probes may artefactually contribute to the oxidation of these dyes by reactive oxygen species induced by vanadium in cells.
The Effects of Multiple Global Change Factors on Soil Nutrients across China: A Meta-Analysis
Authors: Shen Xinyi , Ma Junwei , Li Yuqian , Li Yijia , Xia Xinghui
Published: November 2022

The quantification of the effects of global changes on soil nutrients is crucial for the prediction of future terrestrial ecosystem changes. Combined with 100 articles and 1129 observations from all over China, the meta-analysis method was applied to explore the effects of various global change factors on soil nutrients, including precipitation change, nitrogen addition, warming, and carbon dioxide (CO2) concentration rise. Results indicated that among all the individual drivers, soil nutrients are most sensitive to N addition. Significant positive effects of N addition on carbon concentration (+4.6%), nitrogen concentration (+6.1%), organic carbon (+5.0%), and available nitrogen (+74.6%) were observed considering all the land-use types. The results highlighted that the combined and interactive effects of multiple global change factors on soil nutrients were of great significance. The interaction of the two drivers is usually additive, followed by antagonism and synergy. Our findings contribute to better understanding of how soil nutrients will change under future global change.
Land Use Mix in Functional Urban Areas of Selected Central European Countries from 2006 to 2012
Authors: Kudas Dawid , Wnęk Agnieszka , Tátošová Lucia
Published: November 2022

The land use mix (LUM) is among the critical issues in spatial planning because it can determine the occurrence and structure of various land use and cover types (LUC) and prevent any adverse patterns. The paper focuses on the LUM in functional urban areas (FUAs) in Czechia, Slovakia, Poland, and Hungary. The research employed Urban Atlas (UA) data on LUC in 2006 and 2012 to characterise LUM in the FUAs. The research follows the division of the FUA into the urban area (urban core, UC) and its functional surroundings (commuting zones, CZ). We further characterised the phenomena investigated for the entire country, region, and Europe using Corine Land Cover (CLC) data. The LUM was quantified with the entropy index (EI), dissimilarity index (DI), and multi-dimensional balance index (MBI). The EI demonstrated that the investigated FUAs went through more substantial LUM changes than the 27 European Union member states (EU27) from 2006 to 2012. Moreover, it has been demonstrated that LUM overspill was more intensive in CZs than in UCs on the regional and national levels. We found out that urbanised areas grew at the expense of agricultural areas in both UCs and CZs with similar dynamics in 2006–2012 in all the analysed countries.
Families’ Experiences on Safety Needs of Children with Intellectual Disability
Authors: Modula Mantji Juliah , Sumbane Gsakani Olivia
Published: November 2022

Background: Children with intellectual disability (ID) are known to have a deficit in self-care, social interaction, and learning abilities. Families raising these children experience a range of difficulties that require supportive systems to meet the physical, psychological, and social safety rights of children with ID. The study explored the safety of children with ID through the experiences of their families in the rural Capricorn District of Limpopo Province, South Africa. Methods: In-depth individual interviews and focus group discussion were conducted with 26 families directly involved in raising the children with ID. An inductive thematic analysis of data on the experiences of raising children with ID was undertaken with the aid of ATLAS.ti 8 computer programme. Results: The study revealed that children with ID lack safety at home, schools, and day care centre environments due to a lack of active involvement by nuclear family members, neighbours, and communities, including interaction with their peers and professional service providers in facilities. Safety of children with ID is compromised through exploitation and injuries, leading to marginalisation as they feared further humiliation. Conclusion: The study highlighted that active involvement of family members, communities, and governmental and non-governmental organisations is crucial in ensuring safe environments for children with ID.
Relaxing Effects of Breathing Pseudotsuga menziesii and Lavandula angustifolia Essential Oils on Psychophysiological Status in Older Adults
Authors: Chung Ya-Hui , Chen Shiu-Jen , Lee Ching-Luug , Wu Chun-Wei , Chang Yu-Sen
Published: November 2022

We evaluated the effects of breathing Pseudotsuga menziesii (P. menziesii) and Lavandula angustifolia (L. angustifolia) essential oils (EOs) during a horticultural activity on older adults. A total number of 92 older adult (71.2 ± 7.7 years old) participants were guided through a leaf printing procedure. In the meantime, water vapor and EOs were diffused in an orderly manner. The heart rate variability-related parameters as well as the brain waves were recorded. In addition, we also collected data for the State–Trait Anxiety Inventory-State (STAI-S) questionnaires before and after the whole indoor natural activity program. The physiological parameters including standard deviation of normal to normal intervals, normalized high frequency (nHF), and high alpha wave increased while the normalized low frequency (nLF), the ratio of LF-to-HF power, high beta wave, and gamma wave decreased following the breathing of P. menziesii and L. angustifolia EOs. These changes indicated a relaxing effect of breathing both EOs during a horticultural activity on older adults. Our results demonstrated a beneficial effect of P. menziesii EO which is as good as a well-known relaxant L. angustifolia EO. This notion was supported by the results of STAI-S. Here we developed an indoor natural activity program for older adults to promote physical and mental health.
DORN1 Is Involved in Drought Stress Tolerance through a Ca2+-Dependent Pathway
Authors: Wang Qingwen , Bai Hongbao , Zada Ahmad , Jiao Qingsong
Published: November 2022

Water shortages caused by climate change seriously threaten the survival and production of plants and are also one of the major environmental pressures faced by plants. DORN1 was the first identified purinoceptor for the plant response to extracellular ATP. It has been established that DORN1 could play key roles in a series of biological activities in plants. However, the biological roles of DORN1 and the mechanism remain unclear under drought stress conditions in plants. Here, DORN1 was targeted for knockout by using the CRISPR/Cas 9 system. It was found that the loss function of DORN1 resulted in a significant decrease in the effective quantum yield of PSII [Y(II)], the photochemical quenching coefficient (qP), and the rate of photosynthetic electron transport through PSII (ETR), which reflected plants’ photochemical efficiency. Whereas Y(NO) values showed obvious enhancement under drought stress conditions. Further experimental results showed that the Y(II), qP, and ETR, which reflect plants’ photochemical efficiency, increased significantly with CaCl2 treatment. These results indicated that the drought tolerance of the mutant was decreased, and the exogenous application of calcium ions could effectively promote the drought tolerance of the dorn1 mutant. Transpiration loss controlled by stomata is closely related to drought tolerance, further, we examined the transpirational water loss in dorn1 and found that it was greater than wild-type (WT). Besides, the dorn1 mutant’s stomatal aperture significantly increased compared with the WT and the stomata of dorn1 mutant plants tend to close after CaCl2 treatment. Taken together, our results show that DORN1 plays a key role in drought stress tolerance in plants, which may depend on calcium and calcium-related signaling pathways.
Exploiting ELIOT for Scaffold-Repurposing Opportunities: TRIM33 a Possible Novel E3 Ligase to Expand the Toolbox for PROTAC Design
Authors: Palomba Tommaso , Tassone Giusy , Vacca Carmine , Bartalucci Matteo , Valeri Aurora , Pozzi Cecilia , Cross Simon , Siragusa Lydia , Desantis Jenny
Published: November 2022

The field of targeted protein degradation, through the control of the ubiquitin–proteasome system (UPS), is progressing considerably; to exploit this new therapeutic modality, the proteolysis targeting chimera (PROTAC) technology was born. The opportunity to use PROTACs engaging of new E3 ligases that can hijack and control the UPS system could greatly extend the applicability of degrading molecules. To this end, here we show a potential application of the ELIOT (E3 LIgase pocketOme navigaTor) platform, previously published by this group, for a scaffold-repurposing strategy to identify new ligands for a novel E3 ligase, such as TRIM33. Starting from ELIOT, a case study of the cross-relationship using GRID Molecular Interaction Field (MIF) similarities between TRIM24 and TRIM33 binding sites was selected. Based on the assumption that similar pockets could bind similar ligands and considering that TRIM24 has 12 known co-crystalised ligands, we applied a scaffold-repurposing strategy for the identification of TRIM33 ligands exploiting the scaffold of TRIM24 ligands. We performed a deeper computational analysis to identify pocket similarities and differences, followed by docking and water analysis; selected ligands were synthesised and subsequently tested against TRIM33 via HTRF binding assay, and we obtained the first-ever X-ray crystallographic complexes of TRIM33α with three of the selected compounds.
Adipose Tissue-Derived CCL5 Enhances Local Pro-Inflammatory Monocytic MDSCs Accumulation and Inflammation via CCR5 Receptor in High-Fat Diet-Fed Mice
Authors: Chan Pei-Chi , Lu Chieh-Hua , Chien Hung-Che , Tian Yu-Feng , Hsieh Po-Shiuan
Published: November 2022

The C-C chemokine motif ligand 5 (CCL5) and its receptors have recently been thought to be substantially involved in the development of obesity-associated adipose tissue inflammation and insulin resistance. However, the respective contributions of tissue-derived and myeloid-derived CCL5 to the etiology of obesity-induced adipose tissue inflammation and insulin resistance, and the involvement of monocytic myeloid-derived suppressor cells (MDSCs), remain unclear. This study used CCL5-knockout mice combined with bone marrow transplantation (BMT) and mice with local injections of shCCL5/shCCR5 or CCL5/CCR5 lentivirus into bilateral epididymal white adipose tissue (eWAT). CCL5 gene deletion significantly ameliorated HFD-induced inflammatory reactions in eWAT and protected against the development of obesity and insulin resistance. In addition, tissue (non-hematopoietic) deletion of CCL5 using the BMT method not only ameliorated adipose tissue inflammation by suppressing pro-inflammatory M-MDSC (CD11b+Ly6G−Ly6Chi) accumulation and skewing local M1 macrophage polarization, but also recruited reparative M-MDSCs (CD11b+Ly6G−Ly6Clow) and M2 macrophages to the eWAT of HFD-induced obese mice, as shown by flow cytometry. Furthermore, modulation of tissue-derived CCL5/CCR5 expression by local injection of shCCL5/shCCR5 or CCL5/CCR5 lentivirus substantially impacted the distribution of pro-inflammatory and reparative M-MDSCs as well as macrophage polarization in bilateral eWAT. These findings suggest that an obesity-induced increase in adipose tissue CCL5-mediated signaling is crucial in the recruitment of tissue M-MDSCs and their trans-differentiation to tissue pro-inflammatory macrophages, resulting in adipose tissue inflammation and insulin resistance.
Genomic Profiling of Sarcomas: A Promising Weapon in the Therapeutic Arsenal
Authors: Lopes-Brás Raquel , Lopez-Presa Dolores , Esperança-Martins Miguel , Melo-Alvim Cecília , Gallego Lina , Costa Luís , Fernandes Isabel
Published: November 2022

Sarcomas are rare malignant mesenchymal neoplasms, and the knowledge of tumor biology and genomics is scarce. Chemotherapy is the standard of care in advanced disease, with poor outcomes. Identifying actionable genomic alterations may offer effective salvage therapeutic options when previous lines have failed. Here, we report a retrospective cohort study of sarcoma patients followed at our center and submitted to comprehensive genomic profiling between January 2020 and June 2021. Thirty patients were included, most (96.7%) with reportable genomic alterations. The most common alterations were linked to cell cycle regulation (TP53, CDKN2A/B, and RB1 deletions and CDK4, MDM2, and MYC amplifications). Most patients (96.7%) had microsatellite stability and low tumor mutational burden (≤10 muts/megabase (Mb); median 2 Muts/Mb). Two-thirds of patients had actionable mutations for targeted treatments, including five cases with alterations amenable to targeted therapies with clinical benefit within the patient’s tumor type, ten cases with targetable alterations with clinical benefit in other tumor types, and five cases with alterations amenable to targeting with drugs under investigation in a clinical trial setting. A significant proportion of cases in this study had actionable genomic alterations with available targeted drugs. Next-generation sequencing is a feasible option for identifying molecular drivers that can provide therapeutic options for individual patients. Molecular Tumor Boards should be implemented in the clinical practice to discuss genomic findings and inform clinically relevant targeted therapies.