bluuebunny/crossref_metadata_2025 huggingface dataset:
Morphological and Immunohistochemical Characterization of Bone Structure and Cell–Cell Communication in a Rat Osteoporosis Model
Authors: Glenske Kristina , Eldaey Asmaa , Schaalo Stephanie , Arnhold Stefan , Heiss Christian , Schnettler Reiner , Wenisch Sabine , Elashry Mohamed I.
Published: April 2024

Bone remodeling is essential for maintaining bone health. The imbalance between bone formation and bone resorption leads to bone diseases such as osteoporosis. Connexin43 (Cx43) is a gap junction molecule that plays an important role in bone homeostasis. The present study investigates the morphological characteristics of bone trabeculae and the distribution of Cx43 in bone cells using osteoporotic rat models to explore the relationship between osteoporosis and bone remodeling. Female Sprague–Dawley rats were divided into three groups: sham, ovarectomy with food deprivation (OVX+diet), and ovarectomy with steroid administration (OVX+steroid) for 3 and 12 months to induce osteoporosis. The lumbar vertebrae were processed for histomorphometric and immunohistochemical evaluation of the trabeculae and the distribution of Cx43 in bone cells. The data showed a significant reduction in trabecular bone in both osteoporotic groups. After 12 months, the OVX+diet treatment resulted in reduced mineralization and an increase in unmineralized bone. The percentage of alkaline phosphatase-positive areas in the OVX+diet vertebrae was lower at 12 months compared to the sham group. A significant increase in tartrate-resistant acid phosphatase (TRAP) positive osteoclasts was observed in the OVX+diet group. Both osteoporotic groups showed a decrease in Cx43-positive osteoblasts areas. An increase in the number of osteoclasts positive for Cx43 was detected in the OVX+diet group. The changes in Cx43 distribution in bone cells, together with trabecular mineralization, suggest that Cx43 may play a role in the progression of osteoporosis and could be a valuable target to improve bone remodeling.
Transcriptional and Metabolic Response of a Strain of Escherichia coli PTS− to a Perturbation of the Energetic Level by Modification of [ATP]/[ADP] Ratio
Authors: Soria Sandra , Carreón-Rodríguez Ofelia E. , de Anda Ramón , Flores Noemí , Escalante Adelfo , Bolívar Francisco
Published: April 2024

The intracellular [ATP]/[ADP] ratio is crucial for Escherichia coli’s cellular functions, impacting transport, phosphorylation, signaling, and stress responses. Overexpression of F1-ATPase genes in E. coli increases glucose consumption, lowers energy levels, and triggers transcriptional responses in central carbon metabolism genes, particularly glycolytic ones, enhancing carbon flux. In this contribution, we report the impact of the perturbation of the energetic level in a PTS− mutant of E. coli by modifying the [ATP]/[ADP] ratio by uncoupling the cytoplasmic activity of the F1 subunit of the ATP synthase. The disruption of [ATP]/[ADP] ratio in the evolved strain of E. coli PB12 (PTS−) was achieved by the expression of the atpAGD operon encoding the soluble portion of ATP synthase F1-ATPase (strain PB12AGD+). The analysis of the physiological and metabolic response of the PTS− strain to the ATP disruption was determined using RT–qPCR of 96 genes involved in glucose and acetate transport, glycolysis and gluconeogenesis, pentose phosphate pathway (PPP), TCA cycle and glyoxylate shunt, several anaplerotic, respiratory chain, and fermentative pathways genes, sigma factors, and global regulators. The apt mutant exhibited reduced growth despite increased glucose transport due to decreased energy levels. It heightened stress response capabilities under glucose-induced energetic starvation, suggesting that the carbon flux from glycolysis is distributed toward the pentose phosphate and the Entner–Duodoroff pathway with the concomitant. Increase acetate transport, production, and utilization in response to the reduction in the [ATP]/[ADP] ratio. Upregulation of several genes encoding the TCA cycle and the glyoxylate shunt as several respiratory genes indicates increased respiratory capabilities, coupled possibly with increased availability of electron donor compounds from the TCA cycle, as this mutant increased respiratory capability by 240% more than in the PB12. The reduction in the intracellular concentration of cAMP in the atp mutant resulted in a reduced number of upregulated genes compared to PB12, suggesting that the mutant remains a robust genetic background despite the severe disruption in its energetic level.
RHEED Study of the Epitaxial Growth of Silicon and Germanium on Highly Oriented Pyrolytic Graphite
Authors: Lozovoy Kirill A. , Dirko Vladimir V. , Kukenov Olzhas I. , Sokolov Arseniy S. , Krukovskii Konstantin V. , Snegerev Mikhail S. , Borisov Alexey V. , Kistenev Yury V. , Kokhanenko Andrey P.
Published: April 2024

Two-dimensional silicon (silicene) and germanium (germanene) have attracted special attention from researchers in recent years. At the same time, highly oriented pyrolytic graphite (HOPG) and graphene are some of the promising substrates for growing silicene and germanene. However, to date, the processes occurring during the epitaxial growth of silicon and germanium on the surface of such substrates have been poorly studied. In this work, the epitaxial growth of silicon and germanium is studied directly during the process of the molecular beam epitaxy deposition of material onto the HOPG surface by reflection high-energy electron diffraction (RHEED). In addition, the obtained samples are studied by Raman spectroscopy and scanning electron microscopy. A wide range of deposition temperatures from 100 to 800 °C is considered and temperature intervals are determined for various growth modes of silicon and germanium on HOPG. Conditions for amorphous and polycrystalline growth are distinguished. Diffraction spots corresponding to the lattice constants of silicene and germanene are identified that may indicate the presence of areas of graphene-like 2D phases during epitaxial deposition of silicon and germanium onto the surface of highly oriented pyrolytic graphite.
Cancer and Cardiovascular Disease: The Conjoined Twins
Authors: Zmaili Mohammad , Alzubi Jafar , Alkhayyat Motasem , Albakri Almaza , Alkhalaileh Feras , Longinow Joshua , Moudgil Rohit
Published: April 2024

Cancer and cardiovascular disease are the two most common causes of death worldwide. As the fields of cardiovascular medicine and oncology continue to expand, the area of overlap is becoming more prominent demanding dedicated attention and individualized patient care. We have come to realize that both fields are inextricably intertwined in several aspects, so much so that the mere presence of one, with its resultant downstream implications, has an impact on the other. Nonetheless, cardiovascular disease and cancer are generally approached independently. The focus that is granted to the predominant pathological entity (either cardiovascular disease or cancer), does not allow for optimal medical care for the other. As a result, ample opportunities for improvement in overall health care are being overlooked. Herein, we hope to shed light on the interconnected relationship between cardiovascular disease and cancer and uncover some of the unintentionally neglected intricacies of common cardiovascular therapeutics from an oncologic standpoint.
Therapeutic Potential of Clusterin Inhibition in Human Cancer
Authors: Martín-García Desirée , García-Aranda Marilina , Redondo Maximino
Published: April 2024

Clusterin (CLU) protein is involved in various pathophysiological processes including carcinogenesis and tumor progression. In recent years, the role of the secretory isoform has been demonstrated in tumor cells, where it inhibits apoptosis and favors the acquisition of resistance to conventional treatments used to treat cancer. To determine the possible therapeutic potential of inhibiting this protein, numerous studies have been carried out in this field. In this article, we present the existing knowledge to date on the inhibition of this protein in different types of cancer and analyze the importance it could have in the development of new therapies targeted against this disease.
Sudden Cardiac Death in the Young: State-of-the-Art Review in Molecular Autopsy
Authors: Salzillo Cecilia , Sansone Vincenza , Napolitano Francesco
Published: April 2024

Sudden cardiac death (SCD) is defined as unexpected death due to a cardiac cause that occurs rapidly. Despite the identification of prevention strategies, SCD remains a serious public health problem worldwide, accounting for 15–20% of all deaths, and is therefore a challenge for modern medicine, especially when it affects young people. Sudden cardiac death in young people affects the population aged ≤ 35 years, including athletes and non-athletes, and it is due to various hereditary and non-hereditary causes. After an autopsy, if the cause remains unknown, it is called sudden unexplained death, often attributable to genetic causes. In these cases, molecular autopsy—post-mortem genetic testing—is essential to facilitate diagnostic and therapeutic pathways and/or the monitoring of family members of the cases. This review aims to elaborate on cardiac disorders marked by genetic mutations, necessitating the post-mortem genetic investigation of the deceased for an accurate diagnosis in order to facilitate informed genetic counseling and to implement preventive strategies for family members of the cases.
Influence of a UVA-Activated TiO2 Coating on Bacterial Surface Colonization in Water-Bearing Systems
Authors: Steinhäußer Linda , König Ulla , Fietzke Fred , Gotzmann Gaby
Published: April 2024

This study focuses on the use of superhydrophilic titanium dioxide (TiO2) coatings applied to the surfaces of water-bearing systems to prevent surface colonization and biofilm formation. Biofilms in water-bearing systems are a problem in many industrial areas and are associated with risks to hygiene and health, material damage, and high costs for cleaning and maintenance. We investigated the suitability of TiO2 coatings activated by UVA irradiation to achieve a superhydrophilic surface. The well-adherent coatings were deposited on flat and curved substrates (stainless steel, Al2O3) by pulsed magnetron sputtering. Surface characteristics, wettability, and the influence on microbial surface colonization were evaluated by WCA measurements, SEM, and XRD. For microbiological evaluation, Escherichia coli and Staphylococcus warneri were used. An adapted and specialized regime for sample conditioning and testing was developed that allows comparability with upcoming studies in this field. The superhydrophilicity was stable for up to 4 days, and an additional UVA reactivation step revealed comparable results. The microbiological studies proved a successful prevention of bacterial colonization on the activated coatings, which is attributed to their superhydrophilicity. The results demonstrate the potential of UV-activated TiO2 as a long-term coating of water-bearing systems, like pipes, on which it assists in avoiding biofilm formation.
Influence of Thickness on the Structure and Biological Response of Cu-O Coatings Deposited on cpTi
Authors: Ilievska Ivana , Ivanova Veronika , Dechev Dimitar , Ivanov Nikolay , Ormanova Maria , Nikolova Maria P. , Handzhiyski Yordan , Andreeva Andreana , Valkov Stefan , Apostolova Margarita D.
Published: April 2024

This work presents results on the influence of thickness on the structure and biological response of Cu-O coatings deposited on commercially pure titanium (cpTi) substrates using direct current (DC) magnetron sputtering. The deposition times were 5, 10, and 15 min to obtain coatings with different thicknesses. The results show that the films deposited for 5, 10, and 15 min correspond to thicknesses of 41, 74, and 125 nm, respectively. The phase composition of the coatings is in the form of a double-phase structure of CuO and Cu2O in all considered cases. The roughness is on the nanometric scale and no obvious trend as a function of the thickness can be observed for the deposited films. Also, it was found that, with an increase in the thickness of the films, the distribution of the heights becomes closer to symmetrical. The antimicrobial efficacy of different Cu-O-coated cpTi substrates was examined using a direct contact experiment. A possible bactericidal effect was investigated by inoculating a 200 μL bacterial suspension on CuO-coated cpTi and cpTi (control) for 24 h at 37 °C. The results showed that Cu-O-coated cpTi substrates have a 50%–60% higher antimicrobial activity than the substrate. At the same time, human osteosarcoma (MG-63) cells growing on Cu-O-coated cpTi substrates showed 80% viability following 24 h incubation. Depending on magnetron sputtering process parameters, a different coating thickness, various crystallite phase compositions, and diverse biocompatibility were obtained.
Habitat Selection of Three Neotropical Grassland Birds Is Dependent on Vegetation Structure and Resources
Authors: Peacock Jo , Macleod Ross , Davies G. Matt , Boorsma Tjalle , Tonra Christopher M.
Published: April 2024

Grassland birds are globally imperiled. Those of endemic Neotropical savannas may be particularly threatened as knowledge of the ecology of many species is lacking, restricting our ability to take decisive conservation action. During the dry (non-breeding) season of 2010, we studied the population size, distribution, and habitat associations of the Cock-tailed Tyrant (Alectrurus tricolor), Black-masked Finch (Coryphaspiza melanotis), and Wedge-tailed Grass-finch (Emberiziodes herbicola) across a disturbance-mediated savanna–grassland gradient in Beni, Bolivia. We used distance sampling and surveyed structural and resource-specific habitat features at plots where birds were present versus random locations. Occupancy models identified fine-scale habitat associations. Cock-tailed Tyrant (7.1 ind./km2) specialized on open habitats in areas expected to be heavily inundated in the wet season, avoided trees, and selected tall grassy swards. Black-masked Finch (25.1 ind./km2) occurred across the gradient, associating with tall, forb-rich swards, sparse shrubs, and low levels of fruiting and seeding vegetation. Wedge-tailed Grass-finch (27.9 ind./km2) also occurred across the gradient, particularly associated with tall, forb-rich swards, abundant seeding grasses, and sparse shrubs. Our results offer the first quantitative abundance estimates for these species in Beni, provide vital baselines for future monitoring, and improve knowledge of the ecology and conservation management needs of these species. Importantly, our results suggest that populations of these three grassland birds may be best maintained in heterogenous, mosaic landscapes that can be produced by carefully managed burning and grazing. Further research in the breeding season would facilitate making stronger, more specific management recommendations.
Interactive Learning: Unpacking the Influence of Computer Simulations on Students’ Mathematical Modeling Processes
Authors: Sanjari Azin , Manouchehri Azita
Published: April 2024

Mathematical modeling and use of technology have been recognized as powerful vehicles for advancing mathematical thinking. The use of technology to aid students to gain understanding about real-world mathematical problems has long been a subject of inquiry. Using the extended Blum’s modeling cycle as the theoretical framework, this study explores how computer simulations influence fifth-grade students’ mathematical modeling processes. We investigated interpretations and perceptions of students regarding a situation model, and how these interpretations and perceptions changed as a result of exposure to the simulation. Observing students as they worked with three interactive computer simulations during task-based interviews, we found that animated graphics of the simulations helped the participants to visualize the problem and learn about the effect of different variables and their interactions on the outcomes of the simulations. Students explored simulation environments, ran simulations to observe outcomes of specific settings, and formed conjectures to describe the effect of simulation variables on the outcomes. Analyzing participants’ activities and movements along the extended Blum modeling cycle, we propose a three-stage learning model composed of “exploration and discovery”, “structured inquiry”, and “synthesizing”.
Design of Three Electric Vehicle Charging Tariff Systems to Improve Photovoltaic Self-Consumption
Authors: Etxegarai Garazi , Camblong Haritza , Ezeiza Aitzol , Lie Tek Tjing
Published: April 2024

Electric vehicles (EVs) are emerging as one of the pillars for achieving climate neutrality. They represent both a threat and an opportunity for the operation of the network. Used as flexible loads, they can favor the self-consumption of photovoltaic (PV) energy. This paper presents three EV charging tariff systems (TSs) based on the self-consumption of excess PV energy. The TS objectives are to increase the self-consumption rate (SCR) and thus indirectly decrease the charging cost of the EV users. Two of the proposed TSs correspond to an indirect control of EV charging. The third TS is a hybrid system where the charging power is controlled. The TS is designed using a series of rules that consider the momentary PV surplus and the charging power of each EV. The influence of the TS is simulated by considering real data from a PV collective self-consumption project in the Basque Country (Spain). The TS simulations performed with 6 months of data show a 13.1% increase in the SCR when applying the third TS, reaching an average of 93.09% for the SCR. In addition, the cost of EV charging is reduced by 25%.
Assessment of Hydrogen Energy Industry Chain Based on Hydrogen Production Methods, Storage, and Utilization
Authors: Ziobrowski Zenon , Rotkegel Adam
Published: April 2024

To reach climate neutrality by 2050, a goal that the European Union set itself, it is necessary to change and modify the whole EU’s energy system through deep decarbonization and reduction of greenhouse-gas emissions. The study presents a current insight into the global energy-transition pathway based on the hydrogen energy industry chain. The paper provides a critical analysis of the role of clean hydrogen based on renewable energy sources (green hydrogen) and fossil-fuels-based hydrogen (blue hydrogen) in the development of a new hydrogen-based economy and the reduction of greenhouse-gas emissions. The actual status, costs, future directions, and recommendations for low-carbon hydrogen development and commercial deployment are addressed. Additionally, the integration of hydrogen production with CCUS technologies is presented.
A Review of the Research on the Wide-Band Oscillation Analysis and Suppression of Renewable Energy Grid-Connected Systems
Authors: Liu Zhu , Li Dayi , Wang Weiyu , Wang Jinfeng , Gong Dehuang
Published: April 2024

With the continuous expansion of the scale of power generated by grid-connected renewable energy, the form and operation characteristics of power grids have undergone significant changes, and the power electronic characteristics of power systems are prominent, resulting in the frequent occurrence of wide-band oscillation problems when renewable energy power generation equipment is connected to a power grid. Oscillation has the characteristics of nonlinearity and has an oscillation frequency ranging from a few hertz to several thousand hertz or more, which seriously threatens the stable operation of power grids. This paper summarizes the wide-band oscillation events that occur worldwide under the background of renewable energy access to power grids, classifies the wide-band oscillation events according to the distribution of oscillation frequency bands, and sorts out the characteristics of various oscillations. From the perspectives of the source side and network side, the existing oscillation suppression measures are classified and prospected.
Global Benchmarking and Modelling of Installed Solar Photovoltaic Capacity by Country
Authors: Alghanem Hussah , Buckley Alastair
Published: April 2024

Setting solar photovoltaic capacity targets and implementing supportive policies is a widespread strategy among nations aiming to achieve decarbonisation goals. However, policy implementation without a thorough understanding of the intricate relationship between social, economic, and land-use factors and solar photovoltaic deployment can lead to unintended consequences, including over- or underdeployment and failure to reach targets. To address this challenge, an investigation was conducted into the relationship between 36 factors and solar photovoltaic deployment across 143 countries from 2001 to 2020 using correlation analysis and principal component analysis. From these factors, five key variables were identified that collectively explain 79% of the year-to-year variation in photovoltaic capacity. Using these variables, a neural network model was constructed, enabling the estimation of capacity additions by country with an error of less than 10%. Additionally, a solar photovoltaic deployment index was developed, serving as a benchmark for comparing a country’s actual historical photovoltaic deployment to similar nations. Furthermore, the model’s utility in evaluating the impact of solar photovoltaic policies was explored. Through three distinct use cases—forecasting solar photovoltaic capacity additions, developing a solar photovoltaic deployment index, and assessing the impact of solar photovoltaic policies—the model emerges as a potentially powerful tool for governments and policy makers to assess solar photovoltaic deployment effectively and formulate strategies to promote sustainable solar energy growth.
Self-Oscillating Converter Based on Phase Tracking Closed Loop for a Dynamic IPT System
Authors: Chen Lin , Luo Daqing , Hong Jianfeng , Guan Mingjie , Chen Wenxiang
Published: April 2024

The coupling of converters with resonant networks poses significant challenges for frequency tracking and power control in inductive power transfer (IPT) systems. This paper presents an implementation method that addresses these issues by dividing the system’s operation into two distinct states: self-oscillating and power-injecting. Based on these states, a phase-closed loop is constructed. Within this closed loop, the phase tracking unit detects and tracks frequency drift, while the power regulating unit incorporates an integrator and adopts a control variable to adjust the output power by modifying the duration of the power injecting state. Meanwhile, the oscillating unit operates in the self-oscillating state. Operating in this manner, the system achieves self-oscillation and demonstrates the capability to effectively track and compensate for system variations within a single cycle. A verification prototype has been constructed, and it demonstrates that the converter within it completely decoupled from the resonant network. Experimental results validate that altering the control variable solely affects the duration of the power-injecting state, allowing for independent control of the output power. When the control variable changes from 2.0 V to 3.5 V, the output power changes from 178 W to 519 W while the self-oscillating state remains unchanged. Furthermore, the system accurately tracks frequency changes, even under significant variations in the coupling coefficient or load, without compromising the power injection state. When the air gap changes from 3 cm to 12 cm, the duration of the self-oscillating state changes from 22.1 μs to 26.3 μs, while the power injecting state remains unchanged. This approach exhibits a robust performance, particularly suitable for dynamic IPT systems sensitive to parameter variations.
State of the Art of Renewable Sources Potentialities in the Middle East: A Case Study in the Kingdom of Saudi Arabia
Authors: Di Lorenzo Gianfranco , Stracqualursi Erika , Vescio Giovanni , Araneo Rodolfo
Published: April 2024

The Kingdom of Saudi Arabia is experiencing a surge in electricity demand, with power generation increasing 4 times in 25 years from 1990 to 2014. Despite the abundant primary renewable energy sources, the country has overlooked them in the past in national energy policies. However, in recent years, renewable energy has become a part of the Kingdom of Saudi Arabia’s energy conservation policy due to climate changes, technological progress, economies of scale, and increased competitiveness in supply chains. The Saudi government has created the King Abdullah City for Atomic and Renewable Energy (KACARE) to develop national strategies for effectively utilizing renewable and nuclear energy. This paper reviews the current state of the art of the renewable energy technologies available on the market and evaluates the installation of renewable energy plants near Saudi Arabia’s East Coast for a new town, focusing on technical rather than economic aspects. The paper provides a wide review of the possible technical solutions to exploit the producibility of different renewable sources, considering the challenging climate conditions typical of desert areas. The analysis of a real case study shows a high availability of wind and solar irradiance that allow a net energy production of 354 and 129 GWh, respectively. In addition, the comparison between a typical ground-mounted photovoltaic (PV) system and an emerging floating PV reveals that for the same installed power, occupied area, and environmental conditions, the latter has a 4% greater performance ratio due to the cooling effect of water.
A Control Strategy for Achieving Constant Voltage Output with an Extensive ZVS Operating Range in Bidirectional Wireless EV Charging Systems
Authors: Li Guangyao , Chen Yafei , Zhang Hailong , Xie Junchen , Jo Seungjin , Kim Dong-Hee
Published: April 2024

Variations in the coupling coefficient of loosely coupled transformers and dynamic loads have a significant impact on the overall performance of bidirectional inductive power transfer (BIPT) systems. However, a wide range of load and coupling coefficient variations are common in the actual charging process, which may cause the converter on both sides to operate in a hard switching state, resulting in switching noise, reduced efficiency, and potential safety concerns. In this paper, a triple-phase-shift control (TPSC) strategy is proposed to study the zero-voltage switching (ZVS) operating range and constant-voltage output (CVO) characteristics of the double-side-LCC (DS-LCC) topology. To ensure a CVO over the wide range of coupling coefficient variations, a dual-phase-shift control is introduced for AC voltage matching. Based on this, the third phase-shift angle control between the converters on both sides is introduced to ensure the ZVS realization. Meanwhile, the time-domain model is developed to analyze the rationality of the proposed third phase-shift angle and the ZVS operating range. Finally, the effectiveness of the proposed TPSC strategy is validated through a 1.5 kW experimental prototype with an air gap of 100–150 mm.
Remote Sensing Estimation of Forest Carbon Stock Based on Machine Learning Algorithms
Authors: Cheng Fengyun , Ou Guanglong , Wang Meng , Liu Chang
Published: April 2024

Improving the precision of remote sensing estimation and implementing the fusion and analysis of multi-source data are crucial for accurately estimating the aboveground carbon storage in forests. Using the Google Earth Engine (GEE) platform in conjunction with national forest resource inventory data and Landsat 8 multispectral remote sensing imagery, this research applies four machine learning algorithms available on the GEE platform: Random Forest (RF), Classification and Regression Trees (CART), Gradient Boosting Trees (GBT), and Support Vector Machine (SVM). Using these algorithms, the entire Yunnan Province is classified into seven categories, including broadleaf forest, coniferous forest, mixed broadleaf-coniferous forest, water bodies, built-up areas, cultivated land, and other types. After a thorough comparison, the research reveals that the RF algorithm surpasses others in terms of accuracy and reliability, making it the most suitable choice for estimating aboveground carbon storage in forests using remote sensing data. Therefore, the study used the RF algorithm for both forest classification and the estimation of carbon storage. By extracting remote sensing factors; by using the Pearson correlation coefficient to select the most relevant factors; and by utilizing multiple linear regression, RF regression, and decision tree regression, a model for estimating aboveground carbon stocks in forests was developed. The results indicate that among the four classification algorithms, the RF classifier demonstrates superior performance, with an overall accuracy of 84.96% and a Kappa coefficient of 76.46%. In the RF regression models, the R2 values for the coniferous forest, broadleaf forest, and mixed needle-broadleaf forest models are 0.636, 0.663, and 0.638, respectively. In both RF and CART, the R2 values for the three forest-type models are greater than 0.6, indicating satisfactory model fitting performance. This study aims to explore the possibility of improving the estimation of forest carbon stocks in large-scale areas through fine land use classification. Additionally, the data sources used are completely free, and medium to low resolution can provide a better reference value for practical applications, thereby reducing the cost of utilization.
Effect of Total Mixed Ration on Growth Performance, Rumen Fermentation, Nutrient Digestion, and Rumen Microbiome in Angus Beef Cattle during the Growing and Fattening Phases
Authors: Li Wei , Ye Boping , Wu Baoyun , Yi Xin , Li Xiang , A Runa , Cui Xiaojing , Zhou Zhiyu , Cheng Yang , Zhu Xiaowen , Tang Xiren , Fu Xinyue , Li Ning , Wu Hao , Zhou Zhenming
Published: April 2024

This study investigates the impact of varying concentrate levels in the diets of Angus beef cattle on their performance, nutrient digestion, and metabolism during the growth (7 to 12 months) and fattening (13 to 30 months) phases. Fifteen Angus steers were continuously fed and divided into low-concentrate (L) and high-concentrate (H) groups based on the fattening period and dietary formulations. Throughout each 9-week trial phase, a comprehensive range of parameters was systematically measured, including dry matter intake (DMI), average daily gain (ADG), gain-to-feed ratio (G/F), blood parameters, rumen fluid composition, and microbial diversity. In the fattening phases, an increase in concentrate levels resulted in a significant rise in the cattle’s DMI. Although there was a minor increase in ADG compared to the growing phases, this increase was not statistically significant. The efficiency of nitrogen (N) utilization in the cattle decreased, accompanied by a significant reduction in the apparent digestibility of nutrients. Ruminal fermentation produced more energy substances; however, there was a notable decrease in the abundance of fiber-decomposing microbes (such as the NK4A214_group, Ruminococcus, Papillibacter, and Acetitomaculum) and a significant increase in the abundance of starch-degrading microbes (including Bacteroidota and Prevotellaceae). Additionally, there was a significant reduction in the abundance of immune system-related functional pathways. This suggests that high-concentrate fattening does not necessarily lead to improved growth performance and may negatively affect metabolic health and nutrient digestion.
Effect of Tannic Acid Concentrations on Temperature-Sensitive Sol–Gel Transition and Stability of Tannic Acid/Pluronic F127 Composite Hydrogels
Authors: Lee Jeong Yun , Shin Hyun Ho , Cho Chungyeon , Ryu Ji Hyun
Published: April 2024

Recently, interest in polyphenol-containing composite adhesives for various biomedical applications has been growing. Tannic acid (TA) is a polyphenolic compound with advantageous properties, including antioxidant and antimicrobial properties. Additionally, TA contains multiple hydroxyl groups that exhibit biological activity by forming hydrogen bonds with proteins and biomacromolecules. Furthermore, TA-containing polymer composites exhibit excellent tissue adhesion properties. In this study, the gelation behavior and adhesion forces of TA/Pluronic F127 (TA/PluF) composite hydrogels were investigated by varying the TA and PluF concentrations. PluF (above 16 wt%) alone showed temperature-responsive gelation behavior because of the closely packed micelle aggregates. After the addition of a small amount of TA, the TA/PluF hydrogels showed thermosensitive behavior similar to that of PluF hydrogels. However, the TA/PluF hydrogels containing more than 10 wt% TA completely suppressed the thermo-responsive gelation kinetics of PluF, which may have been due to the hydrogen bonds between TA and PluF. In addition, TA/PluF hydrogels with 40 wt% TA showed excellent tissue adhesion properties and bursting pressure in porcine intestinal tissues. These results are expected to aid in understanding the use of mixtures of TA and thermosensitive block copolymers to fabricate adhesive hydrogels for versatile biomedical applications.
G-Protein Signaling Modulator 2 as a Potential Biomarker in Colorectal Cancer: Integrative Analysis Using Genetic Profiling and Pan-Cancer Studies
Authors: Kadhim Doaa Jawad , Azari Hanieh , Sokhangouy Saeideh Khorshid , Hassanian Seyed Mahdi , Alshekarchi Hawraa Ibrahim , Goshayeshi Ladan , Goshayeshi Lena , Abbaszadegan Mohammad Reza , Khojasteh-Leylakoohi Fatemeh , Khazaei Majid , Gataa Ibrahim Saeed , Peters Godefridus J. , A. Ferns Gordon , Batra Jyotsna , Lam Alfred King-Yin , Giovannetti Elisa , Avan Amir
Published: April 2024

Colorectal cancer (CRC) imposes a significant healthcare burden globally, prompting the quest for innovative biomarkers to enhance diagnostic and therapeutic strategies. This study investigates the G-protein signaling modulator (GPSM) family across several cancers and presents a comprehensive pan-cancer analysis of the GPSM2 gene across several gastrointestinal (GI) cancers. Leveraging bioinformatics methodologies, we investigated GPSM2 expression patterns, protein interactions, functional enrichments, prognostic implications, genetic alterations, and immune infiltration associations. Furthermore, the expression of the GPSM2 gene was analyzed using real-time analysis. Our findings reveal a consistent upregulation of GPSM2 expression in all GI cancer datasets analyzed, suggesting its potential as a universal biomarker in GI cancers. Functional enrichment analysis underscores the involvement of GPSM2 in vital pathways, indicating its role in tumor progression. The prognostic assessment indicates that elevated GPSM2 expression correlates with adverse overall and disease-free survival outcomes across multiple GI cancer types. Genetic alteration analysis highlights the prevalence of mutations, particularly missense mutations, in GPSM2. Furthermore, significant correlations between GPSM2 expression and immune cell infiltration are observed, suggesting its involvement in tumor immune evasion mechanisms. Collectively, our study underscores the multifaceted role of GPSM2 in GI cancers, particularly in CRC, emphasizing its potential as a promising biomarker for prognosis and therapeutic targeting. Further functional investigations are warranted to elucidate its clinical utility and therapeutic implications in CRC management.
Detection and Analysis of Genes Affecting the Number of Thoracic Vertebrae in Licha Black Pigs
Authors: Wang Yuan , Wang Min , He Xiaojin , Dong Ruilan , Liu Hongjiang , Yu Guanghui
Published: April 2024

The number of thoracic vertebrae (NTV) in pigs is an important economic trait that significantly influences pork production. While the Licha black pig is a well-known Chinese pig breed with multiple thoracic vertebrae, the genetic mechanism is still unknown. Here, we performed a selective signal analysis on the genome of Licha black pigs, comparing individuals with 15 NTV versus those with 16 NTV to better understand functional genes associated with NTV. A total of 2265 selection signal regions were detected across the genome, including 527 genes and 1073 QTL that overlapped with the selection signal regions. Functional enrichment analysis revealed that LRP5 and SP5 genes were involved in biological processes such as bone morphogenesis and Wnt protein binding. Furthermore, three genes, LRP8, DEF6, and SCUBE3, associated with osteoblast differentiation and bone formation, were located within or close to the QTL related to bone development and vertebrae number. These five genes were hypothesized to be potential candidates for regulating the NTV trait in Licha black pigs. Our findings revealed several candidate genes that play crucial roles in NTV regulation and provide a theoretical foundation to understand the genetic mechanism of the NTV trait in pig breeding.
Characteristics and Comparative Analysis of the Complete Plastomes of Apostasia fujianica and Neuwiedia malipoensis (Apostasioideae)
Authors: Zheng Qinyao , Wu Yuwei , Ke Shi-Jie , Liu Ding-Kun , Liu Zhong-Jian
Published: April 2024

Apostasioideae, the early divergent subfamily of Orchidaceae, comprises Apostasia and Neuwiedia genera with approximately 20 species. Despite extensive research on Apostasioideae, previous studies have struggled to resolve taxonomic issues, particularly concerning the position of species within this subfamily. Here, we sequenced and annotated plastomes of Apostasia fujianica and Neuwiedia malipoensis, unveiling their phylogenetic relationships and shared plastome features with the other five published plastomes. We identified and analyzed the length, GC content, repeat sequences, and RSCU values of the chloroplast genomes. It is noteworthy that the chloroplast genome of N. malipoensis stands out as the largest among all known chloroplast genomes within the Apostasioideae subfamily, primarily due to contributions from both the LSC and SSC regions. Furthermore, our analysis revealed three unique structural rearrangements located approximately 10k–47k bp (ycf3–trnS-GCU) and 58k–59k bp(accD) in the LSC region and 118k–119k (ndhI) bp in the SSC region of the chloroplast genomes across all five species within the Apostasia genus, which presents a potential avenue for identifying distinctive chloroplast genetic markers, setting them apart from other orchid plants. And a total of four mutational hotspots (rpoC2, atpH, rps4, ndhK, and clpP) were identified. Moreover, our study suggested that Apostasia and Neuwiedia formed a monophyletic group, with Apostasia being sister to Neuwiedia. Within the Apostasia genus, five species were classified into two major clades, represented as follows: (A. odorata (A. shenzhenica and A. fujianica) (A. ramifera and A. wallichii)). These findings hold significance in developing DNA barcoding of Apostasioideae and contribute to the further phylogenetic understanding of Apostasioideae species.
Recombinant Arginine Deiminase from Levilactobacillus brevis Inhibits the Growth of Stomach Cancer Cells, Possibly by Activating the Intrinsic Apoptosis Pathway
Authors: Mendoza Remilyn M. , Song Ji Hoon , Jung Yong Tae , Paik Hyun-Dong , Park Young-Seo , Kang Dae-Kyung
Published: April 2024

The anticancer potential of Levilactobacillus brevis KU15176 against the stomach cancer cell line AGS has been reported previously. In this study, we aimed to analyze the genome of L. brevis KU15176 and identify key genes that may have potential anticancer properties. Among potential anticancer molecules, the role of arginine deiminase (ADI) in conferring an antiproliferative functionality was confirmed. In vitro assay against AGS cell line confirmed that recombinant ADI from L. brevis KU15176 (ADI_br, 5 µg/mL), overexpressed in E. coli BL21 (DE3), exerted an inhibitory effect on AGS cell growth, resulting in a 65.32% reduction in cell viability. Moreover, the expression of apoptosis-related genes, such as bax, bad, caspase-7, and caspase-3, as well as the activity of caspase-9 in ADI_br-treated AGS cells, was higher than those in untreated (culture medium-only) cells. The cell-scattering behavior of ADI_br-treated cells showed characteristics of apoptosis. Flow cytometry analyses of AGS cells treated with ADI_br for 24 and 28 h revealed apoptotic rates of 11.87 and 24.09, respectively, indicating the progression of apoptosis in AGS cells after ADI_br treatment. This study highlights the potential of ADI_br as an effective enzyme for anticancer applications.
Transcriptome Profiling Unveils Key Genes Regulating the Growth and Development of Yangzhou Goose Knob
Authors: Xu Xinlei , Fan Suyu , Ji Wangyang , Qi Shangzong , Liu Linyu , Cao Zhi , Bao Qiang , Zhang Yang , Xu Qi , Chen Guohong
Published: April 2024

Goose is one of the most economically valuable poultry species and has a distinct appearance due to its possession of a knob. A knob is a hallmark of sexual maturity in goose (Anser cygnoides) and plays crucial roles in artificial selection, health status, social signaling, and body temperature regulation. However, the genetic mechanisms influencing the growth and development of goose knobs remain completely unclear. In this study, histomorphological and transcriptomic analyses of goose knobs in D70, D120, and D300 Yangzhou geese revealed differential changes in tissue morphology during the growth and development of goose knobs and the key core genes that regulate goose knob traits. Observation of tissue sections revealed that as age increased, the thickness of the knob epidermis, cuticle, and spinous cells gradually decreased. Additionally, fat cells in the dermis and subcutaneous connective tissue transitioned from loose to dense. Transcriptome sequencing results, analyzed through differential expression, Weighted Gene Co-expression Network Analysis (WGCNA), and pattern expression analysis methods, showed D70-vs.-D120 (up-regulated: 192; down-regulated: 423), D70-vs.-D300 (up-regulated: 1394; down-regulated: 1893), and D120-vs.-D300 (up-regulated: 1017; down-regulated: 1324). A total of 6243 differentially expressed genes (DEGs) were identified, indicating varied expression levels across the three groups in the knob tissues of D70, D120, and D300 Yangzhou geese. These DEGs are significantly enriched in biological processes (BP) such as skin morphogenesis, the regulation of keratinocyte proliferation, and epidermal cell differentiation. Furthermore, they demonstrate enrichment in pathways related to goose knob development, including ECM–receptor interaction, NF-kappa B, and PPAR signaling. Through pattern expression analysis, three gene expression clusters related to goose knob traits were identified. The joint analysis of candidate genes associated with goose knob development and WGCNA led to the identification of key core genes influencing goose knob development. These core genes comprise WNT4, WNT10A, TCF7L2, GATA3, ADRA2A, CASP3, SFN, KDF1, ERRFI1, SPRY1, and EVPL. In summary, this study provides a reference for understanding the molecular mechanisms of goose knob growth and development and provides effective ideas and methods for the genetic improvement of goose knob traits.
Car T Cells in Solid Tumors: Overcoming Obstacles
Authors: Rojas-Quintero Joselyn , Díaz María P. , Palmar Jim , Galan-Freyle Nataly J. , Morillo Valery , Escalona Daniel , González-Torres Henry J. , Torres Wheeler , Navarro-Quiroz Elkin , Rivera-Porras Diego , Bermúdez Valmore
Published: April 2024

Chimeric antigen receptor T cell (CAR T cell) therapy has emerged as a prominent adoptive cell therapy and a therapeutic approach of great interest in the fight against cancer. This approach has shown notorious efficacy in refractory hematological neoplasm, which has bolstered its exploration in the field of solid cancers. However, successfully managing solid tumors presents considerable intrinsic challenges, which include the necessity of guiding the modified cells toward the tumoral region, assuring their penetration and survival in adverse microenvironments, and addressing the complexity of identifying the specific antigens for each type of cancer. This review focuses on outlining the challenges faced by CAR T cell therapy when used in the treatment of solid tumors, as well as presenting optimizations and emergent approaches directed at improving its efficacy in this particular context. From precise localization to the modulation of the tumoral microenvironment and the adaptation of antigen recognition strategies, diverse pathways will be examined to overcome the current limitations and buttress the therapeutic potential of CAR T cells in the fight against solid tumors.
Two-Month Voluntary Ethanol Consumption Promotes Mild Neuroinflammation in the Cerebellum but Not in the Prefrontal Cortex, Hippocampus, or Striatum of Mice
Authors: Berríos-Cárcamo Pablo , Núñez Sarah , Castañeda Justine , Gallardo Javiera , Bono María Rosa , Ezquer Fernando
Published: April 2024

Chronic ethanol exposure often triggers neuroinflammation in the brain’s reward system, potentially promoting the drive for ethanol consumption. A main marker of neuroinflammation is the microglia-derived monocyte chemoattractant protein 1 (MCP1) in animal models of alcohol use disorder in which ethanol is forcefully given. However, there are conflicting findings on whether MCP1 is elevated when ethanol is taken voluntarily, which challenges its key role in promoting motivation for ethanol consumption. Here, we studied MCP1 mRNA levels in areas implicated in consumption motivation—specifically, the prefrontal cortex, hippocampus, and striatum—as well as in the cerebellum, a brain area highly sensitive to ethanol, of C57BL/6 mice subjected to intermittent and voluntary ethanol consumption for two months. We found a significant increase in MCP1 mRNA levels in the cerebellum of mice that consumed ethanol compared to controls, whereas no significant changes were observed in the prefrontal cortex, hippocampus, or striatum or in microglia isolated from the hippocampus and striatum. To further characterize cerebellar neuroinflammation, we measured the expression changes in other proinflammatory markers and chemokines, revealing a significant increase in the proinflammatory microRNA miR-155. Notably, other classical proinflammatory markers, such as TNFα, IL6, and IL-1β, remained unaltered, suggesting mild neuroinflammation. These results suggest that the onset of neuroinflammation in motivation-related areas is not required for high voluntary consumption in C57BL/6 mice. In addition, cerebellar susceptibility to neuroinflammation may be a trigger to the cerebellar degeneration that occurs after chronic ethanol consumption in humans.
The Combined Effects of Azoxystrobin and the Biosurfactant-Producing Bacillus sp. Kol B3 against the Phytopathogenic Fungus Fusarium sambucinum IM 6525
Authors: Walaszczyk Aleksandra , Jasińska Anna , Bernat Przemysław , Różalska Sylwia , Sas-Paszt Lidia , Lisek Anna , Paraszkiewicz Katarzyna
Published: April 2024

This study aimed to evaluate how the combined presence of the synthetic fungicide azoxystrobin (AZ) and the biosurfactant-producing Bacillus sp. Kol B3 influences the growth of the phytopathogenic fungus Fusarium sambucinum IM 6525. The results showed a noticeable increase in antifungal effectiveness when biotic and abiotic agents were combined. This effect manifested across diverse parameters, including fungal growth inhibition, changes in hyphae morphology, fungal membrane permeability and levels of intracellular reactive oxygen species (ROS). In response to the presence of Fusarium and AZ in the culture, the bacteria changed the proportions of biosurfactants (surfactin and iturin) produced. The presence of both AZ and/or Fusarium resulted in an increase in iturin biosynthesis. Only in 72 h old bacterial–fungal co-culture a 20% removal of AZ was noted. In the fungal cultures (with and without the addition of the bacteria), the presence of an AZ metabolite named azoxystrobin free acid was detected in the 48th and 72nd hours of the process. The possible involvement of increased iturin and ROS content in antifungal activity of Bacillus sp. and AZ when used together are also discussed. Biosurfactants were analyzed by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Microscopy techniques and biochemical assays were also used.