bluuebunny/crossref_metadata_2025 huggingface dataset:
Unravelling the roles of Autophagy in OSCC: A renewed perspective from mechanisms to potential applications
Authors: Gou Qiutong , Zheng Ling-Li , Huang Haixia
Published: October 2022

Oral squamous cell carcinoma (OSCC) is associated with a low survival rate and a high disability rate, making it a serious health burden, particularly in Southeast Asian countries. Therefore, improvements in the diagnosis, treatment, and prognosis prediction of OSCC are highly warranted. Autophagy has a significant impact on cancer development. Studies on autophagy in various human cancers have made outstanding contributions; however, the relationship between autophagy and OSCC remains to be explored. This review highlights the roles of autophagy in OSCC and discusses the relationship between autophagy and Epithelial–mesenchymal transition. Considering the lack of OSCC biomarkers, we focus on the studies involving OSCC-related bioinformatics analysis and molecular targets. Based on some classical targets, we summarize several key autophagy-related biomarkers with a considerable potential for clinical application, which may become the hotspot of OSCC research. In conclusion, we elaborate on the interrelationship between autophagy and OSCC and highlight the shortcomings of current studies to provide insights into the potential clinical strategies.
The edge-coupler of fiber-to-chip with ultra-low coupling loss based on double-layer silicon waveguides
Authors: Ma Xiaoyue , Hu Heming , Liu Shiping , Dong Runyu , Fang Qing , Chen Hua
Published: October 2022

The edge-coupler of fiber-to-chip with ultra-low coupling loss is demonstrated on SOI platform. The edge-coupler is consisted of the cantilevered SiO2 waveguide, the amorphous silicon (α-Si) nano taper and the crystal silicon (c-Si) nano tapers. The thin α-Si layer is deposited on the c-Si layer to improve the pattern matching with fiber. The optical input signal from the optical fiber is launched into the suspended SiO2 waveguide, then coupled into the α-Si nano taper at the center of the SiO2 waveguide, and finally coupled into the c-Si nano taper. We characterized the cantilevered edge-coupler using cleaved single-mode optical fiber with a mode field diameter of 10.5 μm. The measured coupling loss is as low as -1.7 dB per facet for TE mode without index matching liquid at 1550 nm. The 1 dB bandwidth is more than 100 nm with 1 dB alignment tolerances of ±2.0 μm in both horizontal and vertical directions. Besides, potential hybrid optical integration could also be allowed with this results in the future.
Modeling hierarchical attention interaction between contexts and triple-channel encoding networks for document-grounded dialog generation
Authors: Cai Yuanyuan , Zuo Min , Xiong Haitao
Published: October 2022

Dialog systems have attracted attention as they are promising in many intelligent applications. Generating fluent and informative responses is of critical importance for dialog systems. Some recent studies introduce documents as extra knowledge to improve the performance of dialog generation. However, it is hard to understand the unstructured document and extract crucial information related to dialog history and current utterance. This leads to uninformative and inflexible responses in existing studies. To address this issue, we propose a generative model of a neural network with an attention mechanism for document-grounded multi-turn dialog. This model encodes the context of utterances that contains the given document, dialog history, and the last utterance into distributed representations via a triple-channel. Then, it introduces a hierarchical attention interaction between dialog contexts and previously generated utterances into the decoder for generating an appropriate response. We compare our model with various baselines on dataset CMU_DoG in terms of the evaluation criteria. The experimental results demonstrate the state-of-the-art performance of our model as compared to previous studies. Furthermore, the results of ablation experiments show the effectiveness of the hierarchical attention interaction and the triple channel for encoding. We also conduct human judgment to evaluate the informativeness of responses and the consistency of responses with dialog history.
High scintillation yield and fast response to alpha particles from thin perovskite films deposited by pulsed laser deposition
Authors: Caricato Anna Paola , Moretto Sandra , Guascito Maria Rachele , Quarta Gianluca , Mazzeo Marco , Favaro Matteo , Rizwan Aziz Muhammad , Provenzano Chiara , Marra Marcella , Cesaria Maura , Polo Matteo , Delgado Jessica , Pino Felix , Martino Maurizio , Calcagnile Lucio , Quaranta Alberto
Published: October 2022

Although the field of solar cells is the most popular application of perovskite materials, their use in radiation detection applications is emerging. The success of perovskites as radiation detectors rests partly on the same material properties that have led to successful optoelectronics applications, meaning that other specific properties, like large stopping power, high mobility lifetime product, fast response, and large bulk resistance, play a role. In this respect, inorganic perovskites are attracting a lot of attention as scintillator materials with performances sensitive to material shape (single crystals, nanocrystals, and thin films) and growth methods. In this study, we report on the morphological, structural, and optical response of thin CsPbBr3-based perovskite films, deposited by Pulsed Laser Deposition (PLD) and post-growth annealed at 350°C in air, following excitation by different particle sources. The annealing treatment resulted in a prompt structural refinement, grain growth, and oxygen bonding to the Pb phase together with an enrichment of the surface in chemo-adsorbed oxygen probably due to Cs–O interactions, as evidenced by X-ray photoelectron spectroscopy. The film behavior under 2 MeV H+ ion beam irradiation at different fluences was analyzed together with its scintillation properties following an interaction with α particles from an Am-241 radioactive source demonstrating a very fast response for an inorganic material (∼5ns) and a photoelectron yield of about ∼47% with respect to a commercial CsI:Tl scintillator.
Cold atom inertial sensors for navigation applications
Authors: Wright Michael J. , Anastassiou Luke , Mishra Chinmaya , Davies James M. , Phillips Alexander M. , Maskell Simon , Ralph Jason F.
Published: October 2022

Quantum sensors based on atom interferometers can provide measurements of inertial quantities with unprecedented accuracy and precision. It has been suggested that this sea change in sensing could provide an inertial navigation capability that is comparable with current satellite based navigation systems. However, the accuracy of sensor measurements is not the only factor that limits the accuracy of inertial navigation systems. In this paper, we explore the fundamental limits to inertial navigation, and explain how quantum inertial sensors could be used to alleviate some of the problems encountered in current classical inertial navigation systems, but not to solve the fundamental instability inherent in inertial navigation methods.
Comprehensive training load monitoring with biomarkers, performance testing, local positioning data, and questionnaires - first results from elite youth soccer
Authors: Haller Nils , Blumkaitis Julia C. , Strepp Tilmann , Schmuttermair Anna , Aglas Lorenz , Simon Perikles , Neuberger Elmo , Kranzinger Christina , Kranzinger Stefan , O’Brien James , Ergoth Bernd , Raffetseder Stefan , Fail Christian , Düring Manfred , Stöggl Thomas
Published: October 2022

Load management, i.e., prescribing, monitoring, and adjusting training load, is primarily aimed at preventing injury and maximizing performance. The search for objective monitoring tools to assess the external and internal load of athletes is of great interest for sports science research. In this 4-week pilot study, we assessed the feasibility and acceptance of an extensive monitoring approach using biomarkers, neuromuscular performance, and questionnaires in an elite youth soccer setting. Eight male players (mean ± SD: age: 17.0 ± 0.6 years, weight: 69.6 ± 8.2 kg, height: 177 ± 7 cm, VO2max: 62.2 ± 3.8 ml/min/kg) were monitored with a local positioning system (e.g., distance covered, sprints), biomarkers (cell-free DNA, creatine kinase), questionnaires, neuromuscular performance testing (counter-movement jump) and further strength testing (Nordic hamstring exercise, hip abduction and adduction). Feasibility was high with no substantial impact on the training routine and no adverse events such as injuries during monitoring. Adherence to the performance tests was high, but adherence to the daily questionnaires was low, and decreased across the study period. Occasional significant correlations were observed between questionnaire scores and training load data, as well as between questionnaire scores and neuromuscular performance. However, due to the small sample size, these findings should be treated with caution. These preliminary results highlight the feasibility of the approach in elite soccer, but also indicate that modifications are needed in further large-scale studies, particularly in relation to the length of the questionnaire.
Discontinuities in understanding follicular development, the ovulatory cycle and the oviposition cycles in the hen: Advances, opportunities, slow downs and complete stops
Authors: Scanes Colin G.
Published: October 2022

There has been considerable progress in understanding follicular development, the ovulatory cycle and the oviposition cycles in the hen. In particular, there have been tremendous advances in understanding follicular development and recruitment of follicles to the hierarchy of large yellow follicles. There is a need to continue to follow the earlier literature while employing present techniques. Early work allowed an understanding of the ovulation and oviposition cycles. Models for ovulation were developed. However, while these have no passed the test of time, there is no present model that fully accounts to the cycles. Earlier work employed ahemoral light cycles to examine ovulation and oviposition cycles. Recent work has demonstrated that clock genes are expressed in the ovary. The control of incubation by prolactin has been largely elucidated in turkeys. There is evidence that other endocrine glands influence female reproduction in birds including the adrenal cortex, thyroid and pineal. However, there is much that remains to be fully understood.
The protective effect of pericytes on vascular permeability after hemorrhagic shock and their relationship with Cx43
Authors: He Shuangshuang , Zhang Zisen , Peng Xiaoyong , Wu Yue , Zhu Yu , Wang Li , Zhou Henan , Li Tao , Liu Liangming
Published: October 2022

Vascular hyperpermeability is a complication of hemorrhagic shock. Pericytes (PCs) are a group of mural cells surrounded by microvessels that are located on the basolateral side of the endothelium. Previous studies have shown that damage to PCs contributes to the occurrence of many diseases such as diabetic retinopathy and myocardial infarction. Whether PCs can protect the vascular barrier function following hemorrhagic shock and the underlying mechanisms are unknown. A hemorrhagic shock rat model, Cx43 vascular endothelial cell (VEC)-specific knockdown mice, and VECs were used to investigate the role of PCs in vascular barrier function and their relationship with Cx43. The results showed that following hemorrhagic shock, the number of PCs in the microvessels was significantly decreased and was negatively associated with an increase in pulmonary and mesenteric vascular permeability. Exogenous infusion of PCs (106 cells per rat) colonized the microvessels and improved pulmonary and mesenteric vascular barrier function. Upregulation of Cx43 in PCs significantly increased the number of PCs colonizing the pulmonary vessels. In contrast, downregulation of Cx43 expression in PCs or knockout of Cx43 in VECs (Cx43 KO mice) significantly reduced PC colonization in pulmonary vessels in vivo and reduced direct contact formation between PCs and VECs in vitro. It has been suggested that PCs have an important protective effect on vascular barrier function in pulmonary and peripheral vessels following hemorrhagic shock. Cx43 plays an important role in the colonization of exogenous PCs in the microvessels. This finding provides a potential new shock treatment measure.
A multi-center study on the association between serum magnesium levels and allostatic load in hemodialysis patients
Authors: Zhang Yingxin , Yang Zhengling , Yang Huan , Li Xiuyong , Liu Zhi , Bai Youwei , Qian Guangrong , Wu Han , Li Ji , Guo Yuwen , Yang Shanfei , Chen Lei , Yang Jian , Han Jiuhuai , Ma Shengyin , Yang Jing , Yu Linfei , Shui Runzhi , Jin Xiping , Wang Hongyu , Zhang Fan , Chen Tianhao , Li Xinke , Zong Xiaoying , Liu Li , Fan Jihui , Wang Wei , Zhang Yong , Shi Guangcai , Wang Deguang , Tao Shuman
Published: October 2022

Objective: Serum magnesium (Mg2+) levels are associated with insulin resistance, hypertension, lipid abnormalities, and inflammation. However, limited studies have indicated the relationship between Mg2+ and multiple system indexes. The purpose of this study was to investigate the association between Mg2+ and allostatic load (AL) in hemodialysis patients.

Methods: A cross-sectional survey was conducted on hemodialysis patients from different centers in Anhui Province, China, between January and December 2020. A total of 3,025 hemodialysis patients were recruited. Their clinical data were measured before hemodialysis. Information was collected by an online self-reported questionnaire and medical record. Serum Mg2+ was divided into three groups by tertiles. A score of AL greater than or equal to 3 was defined as high AL. A binary logistic regression model was applied to examine the relationship between serum Mg2+ and AL.

Results: A total of 1,222 patients undergoing hemodialysis were included, 60% of whom were males (733/1,222). The mean (standard deviation) age of patients was 55.90 (12.75). The median level of serum Mg2+ was 1.22 mmol/L. The rate of high AL levels was 23.4%. Serum Mg2+ was negatively correlated with body mass index, fasting blood glucose (Glu), and C-reactive protein and positively correlated with high-density lipoprotein, low-density lipoprotein, total cholesterol, diastolic blood pressure (DBP), and serum phosphorus. After adjusting for gender, anxiety, diabetes, family residence, lipid-lowering agents, antihypertensive medications, albumin, and Glu, the binary logistic regression model showed that patients with lower levels of serum Mg2+ were more likely have high AL (OR for the T1 group of serum Mg2+:1.945, 95% CI: 1.365–2.773, and OR for the T2 group of serum Mg2+:1.556, 95% CI: 1.099–2.201).

Conclusion: Our data support the hypothesis that higher serum Mg2+ concentrations may contribute to lower health risk in hemodialysis populations. Further randomized controlled trials and cohort studies are warranted to verify whether Mg2+ supplementation could be part of routine examinations in hemodialysis populations.
Soil microbial community assembly and stability are associated with potato (Solanum tuberosum L.) fitness under continuous cropping regime
Authors: Gu Songsong , Xiong Xingyao , Tan Lin , Deng Ye , Du Xiongfeng , Yang Xingxing , Hu Qiulong
Published: October 2022

Continuous cropping obstacles caused by the over-cultivation of a single crop trigger soil degradation, yield reduction and the occurrence of plant disease. However, the relationships among stability, complexity and the assembly process of soil microbial community with continuous cropping obstacles remains unclear. In this study, molecular ecological networks analysis (MENs) and inter-domain ecological networks analysis (IDENs), and a new index named cohesion tools were used to calculate the stability and complexity of soil microbial communities from eight potato cultivars grown under a continuous cropping regime by using the high-throughput sequencing data. The results showed that the stability (i.e., robustness index) of the bacterial and fungal communities for cultivar ZS5 was significantly higher, and that the complexity (i.e., cohesion values) was also significantly higher in the bacterial, fungal and inter-domain communities (i.e., bacterial-fungal community) of cultivar ZS5 than other cultivars. Network analysis also revealed that Actinobacteria and Ascomycota were the dominant phyla within intra-domain networks of continuous cropping potato soil communities, while the phyla Proteobacteria and Ascomycota dominated the correlation of the bacterial-fungal network. Infer community assembly mechanism by phylogenetic-bin-based null model analysis (iCAMP) tools were used to calculate the soil bacterial and fungal communities’ assembly processes of the eight potato cultivars under continuous cropping regime, and the results showed that the bacterial community was mainly dominated by deterministic processes (64.19% - 81.31%) while the fungal community was mainly dominated by stochastic processes (78.28% - 98.99%), indicating that the continuous-cropping regime mainly influenced the potato soil bacterial community assembly process. Moreover, cultivar ZS5 possessed a relatively lower homogeneous selection, and a higher TP, TN, AP and yield than other cultivars. Our results indicated that the soil microbial network stability and complexity, and community assemble might be associated with yield and soil properties, which would be helpful in the study for resistance to potato continuous cropping obstacles.
Adapting wine grape production to climate change through canopy architecture manipulation and irrigation in warm climates
Authors: Yu Runze , Torres Nazareth , Tanner Justin D. , Kacur Sean M. , Marigliano Lauren E. , Zumkeller Maria , Gilmer Joseph Chris , Gambetta Gregory A. , Kurtural Sahap Kaan
Published: October 2022

Grape growing regions are facing constant warming of the growing season temperature as well as limitations on ground water pumping used for irrigating to overcome water deficits. Trellis systems are utilized to optimize grapevine production, physiology, and berry chemistry. This study aimed to compare 6 trellis systems with 3 levels of applied water amounts based on different replacements of crop evapotranspiration (ETc) in two consecutive seasons. The treatments included a vertical shoot position (VSP), two modified VSPs (VSP60 and VSP80), a single high wire (SH), a high quadrilateral (HQ), and a Guyot pruned VSP (GY) combined with 25%, 50%, and 100% ETcwater replacement. The SH had greater yields, whereas HQ was slower to reach full production potential. At harvest in both years, the accumulation of anthocyanin derivatives was enhanced in SH, whereas VSPs decreased them. As crown porosity increased (mostly VSPs), berry flavonol concentration and likewise molar % of quercetin in berries increased. Conversely, as leaf area increased, total flavonol concentration and molar % of quercetin decreased, indicating a preferential arrangement of leaf area along the canopy for overexposure of grape berry with VSP types. The irrigation treatments revealed linear trends for components of yield, where greater applied water resulted in larger berry size and likewise greater yield. 25% ETcwas able to increase berry anthocyanin and flavonol concentrations. Overall, this study evidenced the efficiency of trellis systems for optimizing production and berry composition in Californian climate, also, the feasibility of using flavonols as the indicator of canopy architecture.
Genetic characterization of hull color using BSR-Seq and genome re-sequencing approaches in foxtail millet
Authors: Tian Bohong , Zhang Lixin , Hu Jinghuang , Liu Yanli , Zhou Lulu , Ping Wenchao , Zou Jingwei , Li Hongjie
Published: October 2022

Hull color of foxtail millet is an important indicator of certain nutritional quality parameters. An F2:6 recombinant inbred line (RIL) population developed by crossing a yellow-hulled cultivar Yugu 5 and a brown-hulled cultivar Jigu 31 was used to determine the genetic control of the hull color trait. This population segregated for yellow and brown hull colors in a ratio of 2:1, indicating that hull color is regulated by multiple genetic loci. A bulk segregant analysis-RNA sequencing (BSR-Seq) approach performed using the RNA bulks from 30 lines with brown and yellow hull colors each identified three genomic regions on chromosomes 1 (4,570,517-10,698,955 bp), 2 (40,301,380-46,168,003 bp), and 3 (44,469,860-50,532,757 bp). A new QTL for brown hull color of Jigu 31, QHC.czas1, was detected between bin markers Block43 and Block697 on chromosome 1 with the genetic linkage map constructed by re-sequencing a subset of the 147 RILs. This QTL explained a high level of phenotypic variation ranging from 28.0% to 47.0%. The corresponding genomic region of this QTL in the foxtail millet reference genome overlapped with that detected on chromosome 1 by the BSR-Seq analysis. Nineteen genes associated with biosynthesis of anthocyanin were annotated in this genomic region. Gene Si1g06530 encoding a SANT/Myb domain protein was highly expressed in developing panicles and seeds, which warrants further verification as the candidate gene for the brown color hull of Jigu 31. Moreover, several annotated genes for biosynthesis of anthocyanin were identified in the genomic regions of chromosomes 2 and 3.
The differing responses of central carbon cycle metabolism in male and female Sargassum thunbergii to ultraviolet-B radiation
Authors: Sun Yan , Zang Yu , Chen Jun , Shang Shuai , Wang Jing , Liu Qian , Tang Xuexi
Published: October 2022

The enhancement of ultraviolet-B radiation (UV-B) radiation reaching the Earth’s surface due to ozone layer depletion is an important topic. Macroalgal species growing in the intertidal zone are often directly exposed to UV-B radiation periodically as the tide changes. In order to better understand the response of macroalgae to UV-B stressed condition, we studied the dominant dioecious intertidal macroalgae Sargassum thunbergii. After consecutive UV-B radiation treatments, we used metabonomics models to analyze and compare the maximum photosynthetic electron transport rate (ETRmax), central carbon cycle metabolism (CCCM) gene expression level, CCCM enzymic activities [pyruvate dehydrogenase and citrate synthase (PDH and CS)], and carbon-based metabolite (including pyruvate, soluble sugar, total amino acid, and lipids) content in male and female S. thunbergii. The results showed that under low and high UV-B radiation, the ETRmax values and six targeted CCCM gene expression levels were significantly higher in males than in females. Under high UV-B radiation, only the CS activity was significantly higher in males than in females. There was no significant difference in PDH activity between males and females. The CCCM models constructed using the metabonomics analysis demonstrate that S. thunbergii males and females exhibit obvious gender differences in their responses to UV-B radiation, providing us with a new understanding of the macroalgal gender differences under UV-B radiation, as past investigations always underestimated their diecious characteristics.
Non-structural carbohydrate dynamics and growth in tomato plants grown at fluctuating light and temperature
Authors: Zepeda Ana Cristina , Heuvelink Ep , Marcelis Leo F. M.
Published: October 2022

Fluctuations in light intensity and temperature lead to periods of asynchrony between carbon (C) supply by photosynthesis and C demand by the plant organs. Storage and remobilization of non-structural carbohydrates (NSC) are important processes that allow plants to buffer these fluctuations. We aimed to test the hypothesis that C storage and remobilization can buffer the effects of temperature and light fluctuations on growth of tomato plants. Tomato plants were grown at temperature amplitudes of 3 or 10°C (deviation around the mean of 22°C) combined with integration periods (IP) of 2 or 10 days. Temperature and light were applied in Phase (high temperature simultaneously with high light intensity, (400 μmol m–2 s–1), low temperature simultaneously with low light intensity (200 μmol m–2 s–1) or in Antiphase (high temperature with low light intensity, low temperature with high light intensity). A control treatment with constant temperature (22°C) and a constant light intensity (300 μmol m–2 s–1) was also applied. After 20 days all treatments had received the same temperature and light integral. Differences in final structural dry weight were relatively small, while NSC concentrations were highly dynamic and followed changes of light and temperature (a positive correlation with decreasing temperature and increasing light intensity). High temperature and low light intensity lead to depletion of the NSC pool, but NSC level never dropped below 8% of the plant weight and this fraction was not mobilizable. Our results suggest that growing plants under fluctuating conditions do not necessarily have detrimental effects on plant growth and may improve biomass production in plants. These findings highlight the importance in the NSC pool dynamics to buffer fluctuations of light and temperature on plant structural growth.
ROS scavenging and ion homeostasis is required for the adaptation of halophyte Karelinia caspia to high salinity
Authors: Li Cui , Mur Luis A.J. , Wang Qinghai , Hou Xincun , Zhao Chunqiao , Chen Zhimin , Wu Juying , Guo Qiang
Published: October 2022

The halophyte Karelinia caspia has not only fodder and medical value but also can remediate saline-alkali soils. Our previous study showed that salt-secreting by salt glands is one of main adaptive strategies of K. caspia under high salinity. However, ROS scavenging, ion homeostasis, and photosynthetic characteristics responses to high salinity remain unclear in K. caspia. Here, physio-biochemical responses and gene expression associated with ROS scavenging and ions transport were tested in K. caspia subjected to 100–400 mM NaCl for 7 days. Results showed that both antioxidant enzymes (SOD, APX) activities and non-enzymatic antioxidants (chlorogenic acid, α-tocopherol, flavonoids, polyamines) contents were significantly enhanced, accompanied by up-regulating the related enzyme and non-enzymatic antioxidant synthesis gene (KcCu/Zn-SOD, KcAPX6, KcHCT, KcHPT1, Kcγ-TMT, KcF3H, KcSAMS and KcSMS) expression with increasing concentrations of NaCl. These responses are beneficial for removing excess ROS to maintain a stable level of H2O2 and O2− without lipid peroxidation in the K. caspia response to high salt. Meanwhile, up-regulating expression of KcSOS1/2/3, KcNHX1, and KcAVP was linked to Na+ compartmentalization into vacuoles or excretion through salt glands in K. caspia. Notably, salt can improve the function of PSII that facilitate net photosynthetic rates, which is helpful to growing normally in high saline. Overall, the findings suggested that ROS scavenging systems and Na+/K+ transport synergistically contributed to redox equilibrium, ion homeostasis, and the enhancement of PSII function, thereby conferring high salt tolerance.
Histone modification and chromatin remodeling in plant response to pathogens
Authors: Kang Huijia , Fan Tianyi , Wu Jiabing , Zhu Yan , Shen Wen-Hui
Published: October 2022

As sessile organisms, plants are constantly exposed to changing environments frequently under diverse stresses. Invasion by pathogens, including virus, bacterial and fungal infections, can severely impede plant growth and development, causing important yield loss and thus challenging food/feed security worldwide. During evolution, plants have adapted complex systems, including coordinated global gene expression networks, to defend against pathogen attacks. In recent years, growing evidences indicate that pathogen infections can trigger local and global epigenetic changes that reprogram the transcription of plant defense genes, which in turn helps plants to fight against pathogens. Here, we summarize up plant defense pathways and epigenetic mechanisms and we review in depth current knowledge’s about histone modifications and chromatin-remodeling factors found in the epigenetic regulation of plant response to biotic stresses. It is anticipated that epigenetic mechanisms may be explorable in the design of tools to generate stress-resistant plant varieties.
Transcriptome characteristics during cell wall formation of endosperm cellularization and embryo differentiation in Arabidopsis
Authors: Li Chengcheng , Hu Fan , Chen Hongyu , Zhao Jie
Published: October 2022

Embryonic and endosperm development are important biological events during Arabidopsis seed development, and are controlled by dynamic changes in a range of gene expression. Nevertheless, the regulatory mechanisms of endosperm cellularization and embryo differentiation remain unclear. Here, we characterized the early embryo and endosperm development of the naa15 mutant that had abnormal embryo differentiation and incomplete endosperm cellularization compared to WT of Arabidopsis, and comparatively investigated the changes of gene expressions in WT seeds at 3, 4, and 5 days after pollination (3W, 4W, and 5W) and the white homozygous aborted naa15 seeds at 5, 6, and 7 DAP (5M, 6M, and 7M) from naa15-1/+ siliques using RNA sequencing and qPCR assays. The transcriptome analyses showed that there were 2040 and 3630 differentially expressed genes (DEGs) in 4W (at endosperm cellularization initiation stage and heart embryo stage) vs 3W (at syncytium stage and globular embryo stage), and 5W (at end of endosperm cellularization stage and torpedo embryo stage) vs 4W, respectively. The KEGG and GO analyses showed that lipid metabolic processes and transmembrane transport related to cell wall biogenesis, cell division and differentiation, the plant hormone signaling pathway, photosynthesis, and transcription regulator activity were evidently enriched in WT and naa15. The heatmap and qPCR analyses showed that auxin response genes (ARFs), auxin transport genes (PINs) cytokinin synthesis genes (LOGs), cytokinin dehydrogenase genes (CKXs), cytokinin receptor, transcription factors (MYB, bHLH, MADS-box, and ERF) were significantly downregulated in naa15 compared to WT. A series of cell wall genes annotated to xyloglucan endotransglycosylase/hydrolase, pectin methyl esterase, and pectin methyl esterase inhibitor were also identified in these DEGs. Moreover, using an immunofluorescent assay, the features of cell walls displayed that cellulose fluorescence signals in the embryo and endosperm of naa15 were significantly decreased, and the signals of low- and high- methyl esterification of pectin were also obviously decreased in the endosperm of naa15. In summary, we identified a large number of DEGs and investigated the features of cell walls during endosperm cellularization and embryonic differentiation, which provided important information on transcription and gene expression to reveal their regulatory mechanisms.
Recent progress in bio-mediated synthesis and applications of engineered nanomaterials for sustainable agriculture
Authors: Bora Kainat Amin , Hashmi Saud , Zulfiqar Faisal , Abideen Zainul , Ali Haibat , Siddiqui Zamin Shaheed , Siddique Kadambot H. M.
Published: October 2022

The ever-increasing demand for agricultural food products, medicine, and other commercial sectors requires new technologies for agricultural practices and promoting the optimum utilization of natural resources. The application of engineered nanomaterials (ENMs) enhance the biomass production and yield of food crop while resisting harmful environmental stresses. Bio-mediated synthesis of ENMs are time-efficient, low-cost, environmentally friendly, green technology. The precedence of using a bio-mediated route over conventional precursors for ENM synthesis is non-toxic and readily available. It possesses many active agents that can facilitate the reduction and stabilization processes during nanoparticle formation. This review presents recent developments in bio-mediated ENMs and green synthesis techniques using plants, algae, fungi, and bacteria, including significant contributions to identifying major ENM applications in agriculture with potential impacts on sustainability, such as the role of different ENMs in agriculture and their impact on different plant species. The review also covers the advantages and disadvantages of different ENMs and potential future research in this field.
Enhancing technology innovation performance through alliance capability: The role of standard alliance network and political skill of TMTs
Authors: Jiang Hong , Wang Zhisong , Gao Sipeng , Chen Kaihua , Sheng Fan
Published: October 2022

Given the increasing competition in standards, standard alliances have become a vital choice for enterprises to enhance their competitive advantage. In standard alliances, what decisions must top management teams make to help their enterprises improve their innovation performance? To answer this question, we draw on dynamic capability theory, social network theory, and high-level echelon theory to understand how alliance capabilities and standard alliance networks affect technology innovation performance. We collected questionnaire data from 465 manufacturing enterprises in China, and the empirical findings show that (1) enterprise alliance capabilities and standard alliance networks have a positive impact on technology innovation performance; (2) enterprise alliance capabilities and technology innovation performance are mediated by standard alliance networks; and (3) the political skills of top management teams strengthen this moderating model. The results of this study enrich the literature on standard alliances and provide a reference for enterprises in developing standard alliance strategies, cultivating alliance capabilities, and exercising the requisite political skills of top management teams.
Role of personality traits for entrepreneurial intentions of young entrepreneurs: A case study of higher education institution
Authors: Cao Yuanyuan , Asad Muhammad Mujtaba , Wang Lu , Naz Aisha , Almusharraf Norah
Published: October 2022

Pakistan being a young country is struggling to provide employment opportunities. However, entrepreneurship is a perceived strategy for reducing unemployment. The trend of entrepreneurship is also emerging among university students. Thus, the ratio of entrepreneurial intention and start-ups is also increasing among university students and graduates. Therefore, this study aims to examine the role of personality traits in the entrepreneurial intentions of young entrepreneurs. Considering this, qualitative methodology was employed with the case study as the research design. A single case of a university with three different departments was taken. In total, n = 9 entrepreneurs were purposely selected from business (n = 3), computer science (n = 3), and education (n = 3) departments. The data were collected using semi-structured interviews and documentary analysis of their ventures and success stories. This study followed research ethics, including volunteer participation, confidentiality, and reciprocity. The collected data were analyzed using interpretative phenomenological analysis. The findings reveal three main themes: the desire to be an entrepreneur, learning attitude, and personality traits are the leading factors in the entrepreneurial intentions of students and graduates. However, within these themes, the effect of personality traits (consistency and determination, discipline and locus of control, and risk-taking and tolerance) is found to lead to entrepreneurial intentions among young entrepreneurs. This study concludes that most entrepreneurs believe that the role of personality traits is evident in entrepreneurial intentions. Moreover, the personality traits are further strengthened with entrepreneurial experience and help continue entrepreneurship.
Can China’s ecological civilization strike a balance between economic benefits and green efficiency? A preliminary province-based quasi-natural experiment
Authors: Li Yushan , Liu Baoliu , Zhao Pu , Peng Lin , Luo Zhilin
Published: October 2022

To encourage the building of a development route for ecological civilization construction which commensurates with China’s unique national conditions, early demonstration and pilot ecological civilization zones should be built. This study aims to investigate the effects of ecological civilization construction policies on regional total factor productivity, green total factor productivity, and the methods of action by using panel data from 30 provinces in Mainland China from 2005 to 2020. Our findings indicate that the pilot eco-civilization policies have a more significant effect on the promotion of green total factor production, while the effect on total factor productivity is average. Furthermore, the main purpose of the ecological civilization construction pilot is to improve the level of green innovation, optimise the industrial structure and promote the allocation of factors to achieve a win-win situation for regional economic development and green benefits. Moreover, under different levels of economic growth, the pilot eco-civilization policies have a more significant effect on the promotion of green total factor at various stages of economic growth and industrialization. There are also clear discrepancies in how well ecological civilization construction programmes are implemented. Thus, in order to support high-quality regional economic development, it is crucial to continue to advance and promote the pilot eco-civilization initiatives.
Revisiting students’ foreign language learning demotivation: From concepts to themes
Authors: Gao Lixiang , Liu Honggang
Published: October 2022

Demotivation is a common psychological phenomenon in foreign language learning. Having a good understanding of learners’ foreign language learning demotivation is conducive to enriching the fruits of psychological research on foreign language learning theoretically and practically by exploring effective ways to improve learners’ foreign language learning motivation. Therefore, this study entails an analysis of the selected literature from 2001 to 2021 to interpret the concept of foreign language learning demotivation, illustrate the research topics from the classification of demotivators and their relationships with other psychological factors, and fully describe the research methods and participants. Future research should expand theoretical perspectives, include more participants of different grades, and adopt multiple research methods.
Social relationships and grit in English as a foreign language learning among high school students: A three-wave longitudinal study
Authors: Cui Tianxue , Yang Yanchao
Published: October 2022

This study examined the longitudinal reciprocal relations between students’ grit and their perceptions of social relationships (teacher-student and peer relationships) in studying English as a foreign language (EFL). A total of 2,435 students from Grades 10-11 in China (Mage= 16.40 years old, 54.2% female) participated in the study on three occasions across 1 year. The three-wave cross-lagged analytic model results indicated that (a) peer relationship and grit reciprocally enhanced each other across both intervals; (b) the teacher-student relationship at Time 2 was influenced by Time 1 grit, but not vice versa. Nevertheless, the teacher-student relationship transactionally facilitated each other during the second interval (from Time 2 to Time 3). The multiple-group confirmatory factor analysis (MG-CFA) results indicated that such relations remained stable across gender. The study’s findings contribute to ongoing research delineating the dynamic system between social relationships and grit in EFL learning. It also reveals that males and females benefit similarly during social communications with peers and their English language teachers. Implications for educational practices were discussed.
Examine the Moderating Role of Teacher’s Self-Efficacy in the Relationship Between the Job Satisfaction and Professional Learning Community in China
Authors: Li Qiang-tian
Published: October 2022

Purpose

The study aimed in examining the impact of the professional learning community, and teachers’ self-efficacy on the job satisfaction of teachers. Additionally, the study has also examined the moderating role of teachers’ self-efficacy in the relationship between the professional learning community (PLC) and job satisfaction.

Method

The SEM-PLS is employed for the data analysis. The response rate of the study is 50%. The study is carried out on the primary teachers in China.

Results

Three research questions were developed in the current study. The questions were related to the job satisfaction level of PE teachers, which was far beyond the level of satisfaction. As a result, teachers of PE classes had low performance. It was found by the study that professional learning community, job satisfaction of teachers, and self-efficacy are linked with each other. However, the results are inconclusive because of the limitation of the sample.

Implications

The study has several implications among researchers, practitioners, and teachers.

Significance

The study is among the few earlier studies on the issues related to Job Satisfaction and the professional learning community in China.

Originality/Value

The study has highlighted an important issue related to the Job Satisfaction and professional learning community in China.
Word prediction using closely and moderately related verbs in Down syndrome
Authors: Angulo-Chavira Armando Q. , Castellón-Flores Alejandra M. , Barrón-Martínez Julia B. , Arias-Trejo Natalia
Published: October 2022

People with Down syndrome (DS) have several difficulties in language learning, and one of the areas most affected is language production. Theoretical frameworks argue that prediction depends on the production system. Yet, people with DS can predict upcoming nouns using semantically related verbs. Possibly, prediction skills in people with DS are driven by their associative mechanism rather than by the prediction mechanism based on the production system. This study explores prediction mechanisms in people with DS and their relationship with production skills. Three groups were evaluated in a preferential-looking task: young adults, children with DS, and a typically developing control group paired by sex and mental age. Participants saw two images, a target and a distractor. They also heard a sentence in one of the three conditions: with a verb that was closely related to the object (e.g., “The woman read the book”), with a verb that was moderately related to the object (e.g., “My uncle waited for the bus”), or with a verb that was unrelated to the object (e.g., “My sister threw a broom”). Their productive vocabulary was then measured. In the young adult and typically developing groups, the results showed prediction in sentences with highly and moderately related verbs. Participants with DS, however, showed prediction skills only in the highly related context. There was no influence of chronological age, mental age, or production on prediction skills. These results indicate that people with DS base prediction mainly on associative mechanisms and they have difficulty in generating top-down predictions.
Unveiling the effects of consumers' psychological distance on their reactance and related behavioral outcomes: Do lockdown restrictions matter?
Authors: Chen Xianglan , Duan Yachao , Ittefaq Huma , Duan Yahui
Published: October 2022

This study examined consumers' psychological reactance, which is insufficiently explored in the literature. This research fills the gaps found in the literature reviewed and investigates how the psychological reactance of restaurant consumers developed because of social, temporal, and spatial distancing measures during COVID-19. This study also explored ways in which the restaurant industry can increase its compliance with COVID-19 restrictions in such a situation. We explored the effects of social, temporal, and spatial distancing on psychological reactance. We also identified the moderating effect of lockdown restrictions, which strengthen the psychological reactance developed because of (a) social distancing, (b) temporal distancing, and (c) spatial distancing. We collected data from restaurant consumers in Lahore. This study applied quantitative techniques (i.e., a test of normality, measurement model assessment, structural model assessment, and common method variance). The data were analyzed using IBM SPSS 25 and AMOS 24 and the results were interpreted and presented accordingly. This study added to the literature on psychological reactance, service management, and psychological distance. We could also help the restaurant industry overcome the challenges that occurred during the COVID-19 pandemic and the closures. This study could assist the restaurant industry to understand consumer behavior and attract potential consumers.
Is maternal negative affectivity related to psychosocial behavior of preterm and term-born toddlers through mother–child interaction?
Authors: Krijnen L. J. G. , Verhoeven M. , van Baar A. L.
Published: October 2022

Introduction

Children born moderately to late preterm (MLP) are more prone to psychosocial difficulties than their term-born counterparts. Maternal negative affectivity (NA)–a relatively stable personality trait characterized by the tendency to experience negative thoughts, feelings and emotions–has been related to more psychosocial problems in their offspring, and to a lower quality of mother–child interactions. As MLP children seem more sensitive to their early caregiving environment, they might be more affected by maternal NA and interaction style than their term-born peers. The current study investigated whether maternal NA predicted child’s psychosocial outcomes through quality of mother–child interaction, and if these associations differed between MLP and term-born children.

Methods

The sample consisted of 108 MLP and 92 term-born children and their mothers. At 18 months corrected age, maternal NA was measured using a self-report questionnaire and mother–child interaction was observed during two structured tasks. Five subscales of mother–child interaction were assessed: negative interaction, reciprocal engagement, emotional support, maternal stimulation and mother-led interaction. At 24 months corrected age, social–emotional difficulties, internalizing, and externalizing problems were assessed using mother-report.

Results

For MLP children, maternal NA directly, positively, predicted social–emotional difficulties (b = 0.57) and internalizing problems (b = 0.45), but no mediation effect of mother–child interaction was found. For term-born children, no direct effect but a mediation effect of mother-led interaction was found. Higher levels of maternal NA predicted less mother-led interaction which in turn predicted more problems. Birth status did not moderate any of the relationships, showing that the differences in patterns of effects found within the MLP and term-born group did not reach statistical significance.

Discussion

Maternal NA was found to be a risk factor for psychosocial outcomes in toddlers, either directly for MLP children or indirectly through mother-led interaction for term-born children. These findings suggest that the process through which maternal NA affects psychosocial outcomes may be different for MLP and term-born children. However, as the examined moderation effects of birth status did not reach statistical significance, more research using larger sample sizes is needed to study mother–child interaction in greater detail.