bluuebunny/crossref_metadata_2025 huggingface dataset:
Tenebrio molitor as a Clean Label Ingredient to Produce Nutritionally Enriched Food Emulsions
Authors: Aybar Maribel , Simões Sara , Sales Joana Ride , Santos Joel , Figueira Diogo , Raymundo Anabela
Published: February 2023

Tenebrio molitor flour, a sustainable source of protein and bioactive compounds, was used as a clean label ingredient in order to reformulate a commercial hummus sauce, replacing egg yolk and modified starch, improving its nutritional quality. For this purpose, the impact of different concentrations of insect flour on the sauce was studied. Rheology properties, texture profile analysis, and the microstructure of the sauces were analyzed. Nutritional profile analysis was carried out, as well as bioactivity, namely the total phenolic content and the antioxidant capacity. Sensory analysis was conducted to determine the consumer’s acceptance. At low concentrations (up to 7.5% of T. molitor flour) the sauce structure remained practically unchanged. However, for higher additions of T. molitor (10% and 15%), a loss of firmness, adhesiveness and viscosity was observed. Structure parameters such as elastic modulus (G’) at 1 Hz of the sauces with 10% and 15% were significantly lower than the commercial sauce, indicating a loss of structure caused by Tenebrio flour incorporation. Although the formulation with 7.5% T. molitor flour was not the best rated in the sensory analysis, it showed a higher antioxidant capacity compared to the commercial standard. In addition, this formulation also presented the highest concentration in total phenolic compounds (16.25 mg GAE/g) and significantly increased the content of proteins (from 4.25% to 7.97%) and some minerals, compared to the standard.
In Achilles Tendinopathy the Symptomatic Tendon Differs from the Asymptomatic Tendon While Exercise Therapy Has Little Effect on Asymmetries—An Ancillary Analysis of Data from a Controlled Clinical Trial
Authors: Radovanović Goran , Bohm Sebastian , Arampatzis Adamantios , Legerlotz Kirsten
Published: February 2023

Background: As inter-limb asymmetries can be associated with higher injury risk, we aimed to investigate their role in Achilles tendinopathy patients. Methods: In Achilles tendinopathy patients (n = 41), we assessed inter-limb asymmetries of mechanical, material, and morphological musculoskeletal properties and function and how those were affected by 12 weeks of exercise intervention (high-load protocol, n = 13; Alfredson protocol, n = 11). Moreover, we assessed whether asymmetry reductions correlated with improved Patient-Reported Outcomes (VISA-A score). Results: At baseline, tendinopathic tendons demonstrated lower tendon force (p = 0.017), lower tendon stress (p < 0.0001), larger tendon cross-sectional area (CSA) (p < 0.001), and increased intratendinous (p = 0.042) and tendon overall (p = 0.021) vascularization. For the high-load group, PRE-to-POST asymmetry comparisons revealed an asymmetry increase for the counter-movement jump (CMJ) (p = 0.034) and PRE-to-POST VISA-A score improvements correlated with CSA asymmetry reductions (p = 0.024). Within the Alfredson group, PRE-to-POST VISA-A score improvements correlated with CMJ asymmetry reductions (p = 0.044) and tendon stiffness asymmetry increases (p = 0.037). POST-to-POST in-between group comparisons revealed lower asymmetry in the high-load group for tendon elongation (p = 0.021) and tendon strain (p = 0.026). Conclusions: The tendinopathic limb differs from the asymptomatic limb while therapeutic exercise interventions have little effect on asymmetries. Asymmetry reductions are not necessarily associated with tendon health improvements.
The Influence of Endodontic Lesions on The Clinical Evolution of Odontogenic Sinusitis—A Cohort Study
Authors: Kwiatkowska Marta Aleksandra , Szczygielski Kornel , Brociek-Piłczyńska Agnieszka , Chloupek Aldona , Jurkiewicz Dariusz
Published: February 2023

Endodontic disease with formation of periapical lesions (PALs) is one of the most common causes of chronic odontogenic sinusitis (ODS). It requires close collaboration between otolaryngologists and dentists, but the best sequence of management is still unknown. The aim of the present study is to clarify how radiological characteristics of teeth with PALs and previous root-canal treatment (RCT) influence the clinical evolution of the disease and to define the predictive value of its radiological and endoscopic features in determining the need for further surgical intervention. A total of 68 symptomatic patients with ODS with PALs were included in the study. The evaluation was performed by an otolaryngologist and a dentist based on a medical interview, nasal endoscopy, cold pulp testing and tomography images. Patients were prospectively followed for at least 12 months, during which nasal steroids, saline irrigations and RCT were administered. The criteria of disease improvement were: decrease of symptoms, healed sinonasal mucosa in endoscopy and radiological resolution of periapical radiolucency and sinus inflammation. Results showed that 9 (13%) patients improved after conservative treatment and 59 (87%) required further surgical intervention. Patients who improved after medical treatment and RCT were younger (p = 0.043) and had a greater distance from the top of the periapical lesion to the maxillary sinus’ floor (p = 0.003). When expansion of PALs and bone destruction toward the maxillary sinus was observed on radiological imaging (p = 0.041), and when more than one tooth root was affected (p = 0.004), patients were more likely to require surgical intervention. In conclusion, the more roots that are affected and the closer the top of the PAL is to the maxillary sinus’ floor, the greater the possibility of medical treatment and RCT failure. When the bone destruction extends into the maxillary sinus, patients eventually require both tooth extraction and FESS in order to resolve ODS completely.
Comparative Survival Outcomes of Hyperthermic Intraperitoneal Chemotherapy, Intraperitoneal Chemotherapy and Intravenous Chemotherapy for Primary Advanced Ovarian Cancer: A Network Meta-Analysis
Authors: Tang Qin , Huang Mao , Zhang Jing , Huang Zhen , Wang Linlian , Gong Zhengxin , Tang Liangdan
Published: February 2023

Objective: We aimed to compare the survival outcomes and adverse events of hyperthermic intraperitoneal chemotherapy (HIPEC), intraperitoneal chemotherapy (IP)and intravenous chemotherapy (IP)for primary advanced ovarian cancer. Methods: PubMed, CENTRAL (Cochrane Central Registry of Controlled Trials), Embase, Web of Science and Scopus were searched using multiple terms for primary advanced ovarian cancer, including randomized controlled trials and comparative studies in both Chinese and English (up to date 15 August 2022). Outcomes include overall survival, progression-free survival and adverse events. The data were pooled and reported as hazard ratio (HRs) with 95% confidence intervals. The Newcastle–Ottawa Scales were used to assess the risk of bias in the included comparative study. The Cochrane Collaboration’s Risk of Bias Tool was used for randomized controlled trials. Results: In total, 32 studies, including 6347 patients and 8 different platinum-based chemotherapy regimens, were included in this network meta-analysis. Our analysis results showed that HIPEC2 (carboplatin with area under the curve 10) exhibited a statistically significant OS benefit compared to IV, weekly dose-dense chemotherapy and HIPEC1 (cisplatin with 75/100 mg/m2). Intraperitoneal plus intravenous chemotherapy was associated with a statistically significantly better likelihood of overall survival compared to IV. For progression-free survival, our statistical results only suggest a better progression-free survival in ovarian cancer patients treated with HIPEC1 compared with weekly dose-dense chemotherapy. No evidence of difference was observed between the other comparison groups. Compared with the non-HIPEC group, HIPEC may had a higher incidence of electrolyte disturbances (≥grade 3). Conclusion: Our statistical analysis suggests that the groups receiving HIPEC2 had a better OS than the groups receiving IV, weekly dose-dense chemotherapy and HIPEC1. For PFS, our analysis only showed HIPEC1 is better than IV. Moreover, HIPEC may lead to a higher incidence of electrolyte disturbances (≥grade 3). HIPEC therapy for advanced ovarian cancer is currently controversial.
The Role of IL-6 in Inner Ear Impairment: Evidence from 146 Recovered Patients with Omicron Infected in Tianjin, China
Authors: Chen Yu , Mao Xiang , Kuang Manbao , Zhang Ziyue , Bo Mingyu , Yang Yijing , Lin Peng , Wang Wei , Shen Zhongyang
Published: February 2023

Purpose: To explore the effect of inflammatory factors on inner ear impairment in a sample of Omicron-infected patients with a high rate of vaccination in China. Methods: One hundred and forty-six recovered Omicron-infected patients performed the distortion product otoacoustic emission (DPOAE) test and serum test for inflammatory factors; demographic data and vaccination statuses were collected from the questionnaire. Results: Out of 146 patients, the DPOAE pass rate was 81.5% (119/146). Inner ear impairment was significantly correlated with IL-6 titer. The odds ratio (aOR) was 1.24 (95% CI: 1.04–1.49) after adjusting for age, sex, and vaccine characteristics. Notably, this relationship only existed in the 18–60 years group. There were no significant protective effects of vaccination on inner ear function. Conclusions: Inner ear impairment still exists in Omicron-infected patients, which was significantly correlated with IL-6 titer. This relationship was mainly observed in young and middle-aged people, possibly due to a stronger immune response in this age group. The protective effect of vaccination on the inner ear could not be proved.
SEMAC + VAT for Suppression of Artifacts Induced by Dental-Implant-Supported Restorations in Magnetic Resonance Imaging
Authors: Bohner Lauren , Hanisch Marcel , Parize Hian , Sesma Newton , Kleinheinz Johannes , Meier Norbert
Published: February 2023

The purpose of this study was to assess the feasibility of SEMAC + VAT to reduce artifacts induced by dental implant-supported restorations, such as its impact on the image quality. Dental-implant supported restorations were installed in a dry mandible. Magnetic resonance scans were acquired on a 3-Tesla MRI system. Artifact suppression (SEMAC + VAT) was applied with different intensity modes (weak, moderate, strong). Artifacts assessment was performed by measuring the mandible volume increase in MRI images prior (reference dataset) and after installation of dental implant-supported prosthesis. Image quality was assessed by two examiners using a five-point scale. Inter-examiner concordance and correlation analysis was performed with Cronbach’s alpha and Spearman’s test with a significance level at p = 0.05. Mandible volume increased by 60.23% when no artifact suppression method was used. By applying SEMAC + VAT, the volume increase ranged from 17.13% (strong mode) to 32.77% (weak mode). Visualization of mandibular bone was positively correlated with SEMAC intensity degree. SEMAC + VAT reduced MRI artifacts caused by dental-implant supported restorations. A stronger suppression mode improved visualization of mandibular bone in detriment of the scanning time.
The Characteristics of Dialysis Membranes: Benefits of the AN69 Membrane in Hemodialysis Patients
Authors: Kobayashi Shuzo , Ohtake Takayasu
Published: February 2023

Patients undergoing hemodialysis (HD) experience serious cardiovascular complications, through malnutrition, inflammation, and atherosclerosis. Amputation for peripheral arterial disease (PAD) is more prevalent in patients undergoing HD than in the general population. In addition, revascularization procedures in dialysis patients are often associated with subsequent amputation and high mortality rates. To improve the prognosis of dialysis patients, malnutrition and inflammation must be properly treated, which necessitates a better understanding of the characteristics of dialysis membranes. Herein, the characteristics of several dialysis membranes were studied, with a special reference to the AN69 membrane, noting several similarities to low-density lipoprotein (LDL)-apheresis, which is also applicable for the treatment of PAD. Both systems (LDL-apheresis and AN69) have anti-inflammatory and anti-thrombogenic effects because they use a negatively charged surface for extracorporeal adsorptive filtration from the blood/plasma, and contact phase activation. The concomitant use of both these therapeutic systems may have additive therapeutic benefits in HD patients. Here, we reviewed the characteristics of dialysis membranes and benefits of the AN69 membrane in dialysis patients.
Sialendoscopy-Based Analysis of Submandibular Duct Papillae with a Proposal for Classification
Authors: Aničin Aleksandar , Jerman Anže , Urbančič Jure , Pušnik Luka
Published: February 2023

Identifying a submandibular (Wharton’s) duct punctum often hinders sialendoscopy; however, there is a paucity of evidence on whether the appearance of Wharton’s duct papilla impacts the sialendoscopic procedure. A classification of Wharton’s duct papillae based on the macroscopic appearance, size of dilatation probes, and sialendoscopic approach was proposed herein. The classification describing four main types of papillae, A, B, C, and D, was prospectively evaluated on 351 Wharton’s duct papillae in 315 patients. For each papillae type, the demographic/clinical data, intraoperative complications, and time required for sialendoscope introduction were analyzed. Estuary-like papilla (type A) was commonly seen after spontaneous stone extrusion, had no intraoperative complications noted, and had the shortest time required for the sialendoscope introduction. Normal papilla (type B) was the most frequently observed papilla (48.1%), reflecting diverse underlying pathology, while difficult papilla (type C) was often associated with unfavorable anatomical variations of the mandible or floor of the mouth. Substantially closed papilla (type D) had the highest rate of intraoperative complications, namely, perforation with a false passage, and required the longest time for the sialendoscope introduction. In seven patients (2.0%), the entrance into the duct was feasible only through the fistula, while the sialendoscope introduction failed in eight patients (2.3%). In conclusion, the appearance of Wharton’s duct papillae may be influenced by the underlying pathology. Based on the proposed classification, papilla typology affects the duration of sialendoscope introduction and may influence the frequency of intraoperative complications.
Clinical, Translational and Experimental Pharmacotherapeutics Advances
Authors: He Zhiyao , Yu Lei , Xu Zhijie , Sun Chenyu
Published: February 2023

Pharmacotherapeutics is the prevention and treatment of ailments, whether disorders or diseases, with medication [...]
Malaysian Society of Allergy and Immunology Consensus Statement on Sublingual Immunotherapy in Allergic Rhinitis
Authors: Abdullah Baharudin , Pawankar Ruby , Abdul Latiff Amir Hamzah , Woo Kent Chee Keen , Wüstenberg Eike , Khalid Mohamad Azizul Fitri , Xiang Yeoh Zhi , Husain Salina , Mohammad Nurashikin , Md Shukri Norasnieda
Published: February 2023

Allergic rhinitis (AR) is an IgE-mediated inflammatory disease of the upper airway. AR affects the patients’ quality of life, is a known risk factor for asthma and a socio-economic burden. Allergen-specific immunotherapy (AIT), comprising sublingual immunotherapy (SLIT) and subcutaneous immunotherapy (SCIT), involves administering increasing doses of the causative allergen to induce clinical and immunologic tolerance to the allergens. It is the only currently available treatment for AR that has been proven to induce disease-modifying effects (i.e., long-term remission of allergic symptoms or potential prevention of asthma and new sensitizations). Although AIT is conventionally recommended for patients who are non-responsive to symptom-relieving pharmacotherapy, it is presently recommended as a first-line treatment for patients with moderate to severe AR who prefer a treatment with the potential for long-term remission. In light of the relatively recent implementation of AIT in Malaysia, guidelines on its appropriate indication and application are important to attain optimal outcomes. This consensus statement was developed by an expert group formed by the Malaysian Society of Allergy and Immunology to provide evidence-based recommendations for the practice of AIT in Malaysia. Patient and product selection, choice of AIT, and strategy towards an effective treatment outcome in AIT are presented.
A Practical Approach to Systemic Mastocytosis Complications in Cardiac Surgery: A Case Report and Systematic Review of the Literature
Authors: Suleiman Mathieu N. , Brueckl Valeska , Fechner Jörg , Kaemmerer Ann-Sophie , Wilk Florian , Weyand Michael , Harig Frank
Published: February 2023

(1) Background: Systemic mastocytosis is a rare, non-curable disease with potential life-threatening complications in patients receiving cardiac surgery. (2) Methods: This systematic review of the literature was prompted by the case of a life-threatening anaphylactic reaction during cardiac surgery related to systemic mastocytosis. The search of all types of studies, using several databases (Pubmed, Scopus and Web of Science), was conducted through September 2022 to identify the relevant studies. (3) Results: Twelve studies were included describing cases of patients undergoing cardiac surgery who were diagnosed with systemic mastocytosis. An adverse effect, namely anaphylaxis, has happened in three cases. Different strategies of premedication, intraoperative and postoperative management were used. In our case, the patient was admitted for elective biological aortic valve replacement due to severe aortic stenosis. Intraoperatively, the patient developed an anaphylactic shock during the administration of protamine after separation from the cardiopulmonary bypass. This anaphylaxis reaction was a complication of the pre-existing systemic mastocytosis and could be successfully managed by the administration of epinephrine, antihistamines and corticosteroids. (4) Conclusions: This systematic literature search and case report highlight the importance of careful preoperative planning, as well as coordination between cardiac surgeons, anesthesiologists and hemato-oncological specialists, in patients with rare but complication-prone diseases such as systemic mastocytosis.
Hyaluronic Acid Hydrogel Containing Resveratrol-Loaded Chitosan Nanoparticles as an Adjuvant in Atopic Dermatitis Treatment
Authors: Conte Raffaele , De Luca Ilenia , Valentino Anna , Cerruti Pierfrancesco , Pedram Parisa , Cabrera-Barjas Gustavo , Moeini Arash , Calarco Anna
Published: February 2023

Atopic dermatitis (AD) is a common disease-causing skin inflammation, redness, and irritation, which can eventually result in infection that drastically impacts patient quality of life. Resveratrol (Res) is a natural phytochemical famed for its excellent anti-inflammatory and antioxidant activities. However, it is poorly bioavailable. Thus, a drug delivery system is needed to enhance in vivo bioactivity. Herein, we report the preparation of hyaluronic acid (HA) hydrogels containing resveratrol-loaded chitosan (CS) nanoparticles, their physicochemical analysis, and their potential therapeutic effects in the treatment of AD. Positively charged CS nanoparticles prepared by tripolyphosphate (TPP) gelation showed sizes ranging from 120 to around 500 nm and Res encapsulation efficiency as high as 80%. Embedding the nanoparticles in HA retarded their hydrolytic degradation and also slowed resveratrol release. Resveratrol released from nanoparticle-loaded hydrogel counteracted the oxidative damage induced by ROS generation in TNF-α/INF-γ-treated human keratinocytes (HaCaT) used as an AD in vitro model. Moreover, pre-treatment with Res@gel reduced secretion and gene expression of proinflammatory cytokines in HaCaT cells. The physicochemical analysis and in vitro assay confirmed that the formulated hydrogel could be considered an efficient and sustained resveratrol delivery vector in AD treatment.
Geospatiotemporal and Causal Inferential Study of European Epidemiological Patterns of Cannabis- and Substance-Related Congenital Orofacial Anomalies
Authors: Reece Albert Stuart , Hulse Gary Kenneth
Published: February 2023

Introduction. Since high rates of congenital anomalies (CAs), including facial CAs (FCAs), causally attributed to antenatal and community cannabis use have been reported in several recent series, it was of interest to examine this subject in detail in Europe. Methods. CA data were taken from the EUROCAT database. Drug exposure data were downloaded from the European Monitoring Centre for Drugs and Drug Addiction (EMCDDA). Income was taken from the World Bank’s online sources. Results. On the bivariate maps of both orofacial clefts and holoprosencephaly against resin, the Δ9-tetrahydrocannabinol concentration rates of both covariates increased together in France, Bulgaria, and the Netherlands. In the bivariate analysis, the anomalies could be ranked by the minimum E-value (mEV) as congenital glaucoma > congenital cataract > choanal atresia > cleft lip ± cleft palate > holoprosencephaly > orofacial clefts > ear, face, and neck anomalies. When nations with increasing daily use were compared to those without, the former had generally higher rates of FCAs (p = 0.0281). In the inverse probability weighted panel regression, the sequence of anomalies—orofacial clefts, anotia, congenital cataract, and holoprosencephaly—had positive and significant cannabis coefficients of p = 2.65 × 10−5, 1.04 × 10−8, 5.88 × 10−16, and 3.21 × 10−13, respectively. In the geospatial regression, the same series of FCAs had positive and significant regression terms for cannabis of p = 8.86 × 10−9, 0.0011, 3.36 × 10−8, and 0.0015, respectively. Some 25/28 (89.3%) E-value estimates and 14/28 (50%) mEVs were >9 (considered to be in the high range), and 100% of both were >1.25 (understood to be in the causal range). Conclusion. Rising cannabis use is associated with all the FCAs and fulfils the epidemiological criteria for causality. The data indicate particular concerns relating to brain development and exponential genotoxic dose-responses, urging caution with regard to community cannabinoid penetration.
Factors Associated with Adherence to Treatment in Patients with HIV and Diabetes Mellitus
Authors: Rivera-Picón Cristina , Benavente-Cuesta María , Quevedo-Aguado María , Sánchez-González Juan , Rodríguez-Muñoz Pedro
Published: February 2023

We aim to identify the factors that influence the therapeutic adherence of subjects with chronic disease. The design followed in this work was empirical, not experimental, and cross-sectional with a correlational objective. The sample consisted of a total of 400 subjects (199 patients with HIV and 201 patients with diabetes mellitus). The instruments applied for data collection were a sociodemographic data questionnaire; the Morisky, Green, and Levine Medication Adherence Scale (MGL); and the Coping Strategies Questionnaire. In the group of subjects with HIV, the use of emotional coping strategies was related to lower adherence to treatment. On the other hand, in the group of subjects with diabetes mellitus, the variable related to compliance with treatment was the duration of illness. Therefore, the predictive factors of adherence to treatment were different in each chronic pathology. In the group of subjects with diabetes mellitus, this variable was related to the duration of the disease. In the group of subjects with HIV, the type of coping strategy used predicted adherence to treatment. From these results, it is possible to develop health programs to promote issues ranging from nursing consultations to the adherence and treatment of patients with HIV and diabetes mellitus.
Reproducibility of Mesopic and Photopic Pupil Sizes in Myopic Children Using a Dedicated Pupillometer with Human-Assisted or Automated Reading
Authors: Hvid-Hansen Anders , Bækgaard Per , Jacobsen Nina , Hjortdal Jesper , Møller Flemming , Kessel Line
Published: February 2023

This study aimed to investigate the reproducibility of pupil size measurements over time and between reading methods when comparing human-assisted reading to automated reading. Pupillary data were analyzed on a subset of myopic children enrolled in a multicenter randomized clinical trial on myopia control with low-dose atropine. Pupil size measurements were obtained prior to randomization at two time points (screening and baseline visits) using a dedicated pupillometer under mesopic and photopic conditions. A customized algorithm was built to perform automated readings, allowing comparisons between human-assisted and automated readings. Reproducibility analyses followed the principles of Bland and Altman and included the calculation of the mean difference between measurements and limits of agreement (LOA). We included 43 children. Mean (standard deviation) age was 9.8 (1.7) years and 25 (58%) children were girls. Using human-assisted readings, reproducibility over time showed mesopic mean difference of 0.02 mm with LOA from −0.87 mm to 0.91 mm, whereas photopic mean difference was −0.01 mm with LOA from −0.25 mm to 0.23 mm. Reproducibility between human-assisted and automated readings was also higher under photopic conditions, with mean difference of 0.03 mm and LOA from −0.03 mm to 0.10 mm at screening and mean difference of 0.03 mm and LOA from −0.06 mm to 0.12 mm at baseline. Using a dedicated pupillometer, we found that examinations performed under photopic conditions demonstrated higher reproducibility over time and between reading methods. We speculate whether mesopic measurements are sufficiently reproducible to be monitored over time. Furthermore, photopic measurements may be of greater relevance when evaluating the side effects of atropine treatment, such as photophobia.
Association between COVID-19 and Postoperative Neurological Complications and Antipsychotic Medication Use after Cancer Surgery: A Retrospective Study
Authors: Cata Juan P. , Hu Jian , Feng Lei , Chung Caroline , Woodman Scott E. , Meyer Larissa A.
Published: February 2023

Introduction: Millions of Americans infected with the severe acute respiratory syndrome-associated coronavirus-19 (COVID-19) need oncologic surgery. Patients with acute or resolved COVID-19 illness complain of neuropsychiatric symptoms. How surgery affects postoperative neuropsychiatric outcomes such as delirium is unknown. We hypothesize that patients with a history of COVID-19 could have an exaggerated risk of developing postoperative delirium after undergoing major elective oncologic surgery. Methods: We conducted a retrospective study to determine the association between COVID-19 status and antipsychotic drugs during postsurgical hospitalization as a surrogate of delirium. Secondary outcomes included 30 days of postoperative complications, length of stay, and mortality. Patients were grouped into pre-pandemic non-COVID-19 and COVID-19-positive groups. A 1:2 propensity score matching was used to minimize bias. A multivariable logistic regression model estimated the effects of important covariates on the use of postoperative psychotic medication. Results: A total of 6003 patients were included in the study. Pre- and post-propensity score matching demonstrated that a history of preoperative COVID-19 did not increase the risk of antipsychotic medications postoperatively. However, respiratory and overall 30-day complications were higher in COVID-19 individuals than in pre-pandemic non-COVID-19 patients. The multivariate analysis showed that the odds of using postoperative antipsychotic medication use for the patients who had COVID-19 compared to those who did not have the infection were not significantly different. Conclusion: A preoperative diagnosis of COVID-19 did not increase the risk of postoperative antipsychotic medication use or neurological complications. More studies are needed to reproduce our results due to the increased concern of neurological events post-COVID-19 infection.
Is the Non-Coding RNA miR-195 a Biodynamic Marker in the Pathogenesis of Head and Neck Squamous Cell Carcinoma? A Prognostic Meta-Analysis
Authors: Dioguardi Mario , Spirito Francesca , Caloro Giorgia Apollonia , Lo Muzio Lorenzo , Cantore Stefania , Ballini Andrea , Scacco Salvatore , Malcangi Annarita , Sembronio Salvatore , Cascardi Eliano , Arrigoni Roberto , Di Cosola Michele , Nocini Riccardo
Published: February 2023

Head and neck squamous cell carcinoma (HNSCC) represents a heterogeneous group of neoplasms whose histological derivation comes from the mucous membranes lining the epithelium: the oral cavity, the larynx, the hypopharynx, the nasopharynx, and the oropharynx. The etiopathogenetic mechanisms involving tumor genesis including the alteration of cell proliferation, apoptosis, invasion, migration, and death may involve alterations in the expression of microRNA (miR). To date there have been no systematic reviews with meta-analysis conducted specifically on the role of miR-195 in HNSCC; therefore, our hypothesis was to evaluate if the aberrant expression of miR-195 in HNSCC tissues may represent a prognostic biomarker of survival through the hazard ratio (HR) and relative risk (RR) analysis. The systematic review was designed according to the PRISMA indications; in total, three electronic databases were consulted (PubMed, Scopus, Cochrane Central Trial) including Google Scholar and the gray literature, and a combination of keywords was used such as miR-195 AND HNSCC, microRNA AND HNSCC and miR-195. The meta-analysis and trial sequential analysis were performed using RevMan 5.41 software and TSA software (Cochrane Collaboration, Copenhagen, Denmark). This search identified 1592 articles and, at the end of the selection process, three articles were included. The results of the meta-analysis reported an aggregated risk ratio for overall survival (OS) between the expression of miR-195 at the highest and lowest of 0.36 and 6, respectively, 95% CI: [0.25, 0.51]. Heterogeneity was evaluated through Chi2 = 0.05 df = 2 (p = 0.98) and the Higgins index I2 = 0%. The test for the overall effect was Z = 5.77 (p < 0.00001). The forest plot was in favor of higher OS in patients with high miR-195 expression.
Monocyte/HDL Cholesterol Ratios as a New Inflammatory Marker in Patients with Schizophrenia
Authors: Kılıç Nülüfer , Tasci Gulay , Yılmaz Seda , Öner Pınar , Korkmaz Sevda
Published: February 2023

Purpose: Monocyte/HDL cholesterol ratio (MHR) is a novel inflammatory marker that is used as a prognostic factor for cardiovascular diseases and has been studied in many diseases. The aim of this study was to investigate the role of inflammatory factors in schizophrenia patients by examining MHR levels and to compare schizophrenia patients and healthy controls in terms of cardiovascular disease risk. Method: A total of 135 participants between the ages of 18–65, 85 diagnosed with schizophrenia, and 50 healthy individuals in the control group were included in this cross-sectional study. Venous blood samples were taken from the participants and CBC parameters and lipid profiles were analyzed. The sociodemographic and clinical data form and positive and negative symptoms scale (PANSS) were administered to all participants. Results: Although monocyte levels were significantly higher in the patient group, HDL-C levels were lower at significant levels. MHR was found to be higher in the patient group compared to the control group at significant levels. When compared to the control group, total cholesterol, triglyceride, WBC, neutrophil, basophil, and platelet levels were higher in the patient group at significant levels, and RBC, hemoglobin, and hematocrit levels were significantly lower. Conclusion: The elevated MHR in patients with schizophrenia may contribute to our understanding that inflammation plays important roles in the pathophysiology of schizophrenia. Additionally, knowing the levels of MHR and considering the recommendations, such as diet and exercise, in the treatment approaches made us think that it might be beneficial in protecting schizophrenia patients against cardiovascular diseases and early death.
A Systematic Review of Chest Imaging Findings in Long COVID Patients
Authors: Bazdar Somayeh , Kwee Anastasia K. A. L. , Houweling Laura , de Wit-van Wijck Yolanda , Mohamed Hoesein Firdaus A. A. , Downward George S. , Nossent Esther J. , Maitland-van der Zee Anke H.
Published: February 2023

Long COVID is the persistence of one or more COVID-19 symptoms after the initial viral infection, and there is evidence supporting its association with lung damage. In this systematic review, we provide an overview of lung imaging and its findings in long COVID patients. A PubMed search was performed on 29 September 2021, for English language studies in which lung imaging was performed in adults suffering from long COVID. Two independent researchers extracted the data. Our search identified 3130 articles, of which 31, representing the imaging findings of 342 long COVID patients, were retained. The most common imaging modality used was computed tomography (CT) (N = 249). A total of 29 different imaging findings were reported, which were broadly categorized into interstitial (fibrotic), pleural, airway, and other parenchymal abnormalities. A direct comparison between cases, in terms of residual lesions, was available for 148 patients, of whom 66 (44.6%) had normal CT findings. Although respiratory symptoms belong to the most common symptoms in long COVID patients, this is not necessarily linked to radiologically detectable lung damage. Therefore, more research is needed on the role of the various types of lung (and other organ) damage which may or may not occur in long COVID.
Residential Adoption of Best Landscape Management Practices: Effects of Outreach to Reduce Non-Point Source Pollution
Authors: Hayden Lillian , Oki Lorence R. , Haver Darren , Majcherek Tamara , Windbiel-Rojas Karey , Cadenasso Mary L.
Published: February 2023

Urban waterways degradation due to runoff from residential areas can be reduced by adopting best management practices (BMPs) for irrigation, fertilizer, and pesticide use. Although stormwater runoff from urban areas has been studied extensively, we focus on single-family residential land use specifically. Outreach to individual households may have a measurable impact since decisions are being made here. We surveyed households to evaluate the effectiveness of education and outreach campaigns on self-reported use of water and chemicals and evaluated whether self-reported behaviors were reflected in the quality and quantity of water draining from the study areas before and after outreach efforts. Our research was conducted in California, which has a Mediterranean climate with distinct wet and dry periods. Runoff from residential landscapes during the dry season enters waterways undiluted by rainwater, making this runoff particularly detrimental to receiving waters. No significant differences in behavior and BMP adoption from before and after the education and outreach campaign was found. These results are not atypical and may be explained by several factors including the population approach to the survey, lag times between outreach and measurable effects, and the need for a critical threshold of adoption to be met for effects to be measurable.
Use of Satellite Images to Determine the Temperature of Urban Surfaces for Landscape Management Purposes, Case Study Bratislava (Slovak Republic)
Authors: Šalkovič Martin , Pauditšová Eva
Published: February 2023

This contribution deals with the use of data obtained from Landsat 8 satellite imaging to identify surface temperature variability in the example of the city of Bratislava, with an emphasis on identifying hotspots outside the built-up area, for example, on agricultural land—locations which are part of the European Network of Protected Areas. Surface temperature variability is presented in two time periods, on the daytime image taken on 26 July 2021 and on the nighttime image from 28 June 2021. Surface temperature is projected in a profile cut of the area. It vertically illustrates the temperatures of individual types of surfaces. Surfaces are classified by Urban Atlas classes. Areas reflecting the spatial distribution of the residential development in the city of Bratislava have been identified by satellite images in the studied area, and they represent a phenomenon of the urban heat island. Such areas were also identified outside the built-up area, in agricultural areas. The results of our research show that it is important to deal with UHI outside the built-up areas of cities and to orient the attention the territory planning and also to the proposal of measures for the management of these areas. Especially if these areas also include territories of the European system of protected areas, as it is in the case of Bratislava city (e.g., SPA029 Sysľovské polia). The results of reducing the impacts of climate change in cities concern not only the residents. In spatial planning, it is also necessary to address the management of non-built-up areas—localities with a quasi-natural character (e.g., areas with diverse vegetation cover). In order to recognize UHI within residential areas, it is essential to identify areas with significant differences between daytime and nighttime surface temperatures. Large differences between night and daytime surface temperatures can be seen in areas outside the built-up area in Bratislava on arable land where the difference is up to 8.0 °C (in the continuous housing class where the proportion of impermeable surfaces is higher than 80% with a temperature difference of 7.6 °C). Identification of overheated surfaces in the territory makes an important basis for modification of the landscape management and management of nature protection areas. It is important to propose measures related to the reduction in the negative impacts of climate change on the landscape and biodiversity.
Dynamic of the Soil Microbiota in Short-Term Crop Rotation
Authors: Zverev Aleksei O. , Kurchak Oksana N. , Orlova Olga V. , Onishchuk Olga P. , Kichko Arina A. , Eregin Aleksander V. , Naliukhin Aleksey N. , Pinaev Aleksandr G. , Andronov Evgeny E.
Published: February 2023

Crop rotation is one of the oldest and most effective methods of restoring soil fertility, which declines when the same plant is grown repeatedly. One of the reasons for a reduction in fertility is the accumulation of pathogenic and unfavorable microbiota. The modern crop rotation schemes (a set of plant species and their order in the crop rotation) are highly effective but are designed without considering soil microbiota dynamics. The main goal of this study was to perform a short-term experiment with multiple plant combinations to access the microbiological effects of crop rotation. It could be useful for the design of long-term crop rotation schemes that take the microbiological effects of the crop rotation into account. For the analysis, five plants (legumes: vetch, clover, and cereals: oats, wheat, and barley) were used. These five plants were separately grown in pots with soil. After the first phase of vegetation, the plants were removed from the soil and a new crop was planted. Soil samples from all 25 possible combinations of primary and secondary crops were investigated using v4-16S rDNA gene sequencing. It was shown that the short-term experiments (up to 40 days of growing) are effective enough to find microbial shifts in bulk soil from different plants. Both primary and secondary cultures are significant factors for the microbial composition of microbial soil communities. Changes are the most significant in the microbial communities of vetch soils, especially in the case of vetch monoculture. Growing clover also leads to changes in microbiota, especially according to beta-diversity. Data obtained can be used to develop new crop rotation schemes that take into account the microbiological effects of various crops.
Protein and Lipid Content of Milk Extracellular Vesicles: A Comparative Overview
Authors: Buratta Sandra , Urbanelli Lorena , Tognoloni Alessia , Latella Raffaella , Cerrotti Giada , Emiliani Carla , Chiaradia Elisabetta
Published: February 2023

The characterization of the protein and lipid cargo of milk extracellular vesicles from different mammal species is crucial for understanding their biogenesis and biological functions, as well as for a comprehensive description of the nutritional aspects of animal milk for human diet. In fact, milk EVs have been reported to possess relevant biological effects, but the molecules/biochemical pathways underlying these effects have been poorly investigated. The biochemical characterization is an important initial step for the potential therapeutic and diagnostic use of natural or modified milk EVs. The number of studies analysing the protein and lipid composition of milk EVs is limited compared to that investigating the nucleic acid cargo. Here, we revised the literature regarding the protein and lipid content of milk EVs. Until now, most investigations have shown that the biochemical cargo of EVs is different with respect to that of other milk fractions. In addition, even if these studies derived mostly from bovine and human milk EVs, comparison between milk EVs from different animal species and milk EVs biochemical composition changes due to different factors including lactation stages and health status is also beginning to be reported.
Mild Hyperbaric Oxygen Exposure Enhances Peripheral Circulatory Natural Killer Cells in Healthy Young Women
Authors: Nisa Badur Un , Nakanishi Ryosuke , Tanaka Minoru , Lin Hao , Hirabayashi Takumi , Maeshige Noriaki , Kondo Hiroyo , Fujino Hidemi
Published: February 2023

Mild hyperbaric oxygen (HBO) enhances oxygen absorption in blood, relieving fatigue without causing oxidative stress. The benefits of mild HBO have been recognized in the treatment of lifestyle-related diseases and hypertension, but no research has been conducted on its effects on immunity. The aim of the present study is to investigate the effect of mild HBO on natural killer (NK) cells and cytokines in healthy young women. This crossover randomized control trial was conducted with 16 healthy young women. Participants were randomly exposed to normobaric oxygen (NBO; 1.0 atmospheres absolute (ATA), 20.8% oxygen) and mild HBO conditions (1.4 ATA, 35–40% oxygen, injected 18L oxygen per minute) in a hyperbaric oxygen chamber for 70 min. Heart rate, parasympathetic activity, NK cell count, interleukin (IL)-6, IL-12p70 and derivatives of reactive oxygen metabolites (d-ROMs) were measured before and after both exposures. In the NBO condition, parasympathetic activity remained unchanged, whereas after mild HBO exposure, parasympathetic activity was significantly increased. NK cells remained unchanged after NBO exposure, while NK cells were increased after exposure to mild HBO. Exposure to mild HBO did not increase d-ROM values, IL-6 and IL-12p70 protein levels. These findings suggest that exposure to mild HBO can be a useful protocol to increase NK cells by regulating parasympathetic activity via increasing oxygen delivery.
High-Pressure Thermophysical Properties of Eight Paraffinic, Naphthenic, Polyalphaolefin and Ester Base Oils
Authors: Villamayor Antía , Guimarey María J. G. , Mariño Fátima , Liñeira del Río José M. , Urquiola Francisco , Urchegui Raquel , Comuñas María J. P. , Fernández Josefa
Published: February 2023

In this work, the thermophysical properties of four mineral (paraffinic and naphthenic) and four synthetic (polyalphaolefin and ester) base oils are measured. Knowledge of these properties is of vital importance for the correct and optimal formulation and design of lubricants, and for the development of equations of state and transport models that adequately represent their properties. Density, isothermal compressibility, thermal expansion coefficient, dynamic viscosity, pressure–viscosity coefficient, and contact angle were determined. To carry out this work, a pρT apparatus, a rotational viscometer, a falling body viscometer, and a contact angle analyzer were used. Highest densities were found for the polyalphaolefin and ester synthetic oils, increasing around 5% from 0.1 to 100 MPa for all the base oils. The density of the synthetic oils is less dependent on temperature changes. For the expansivity and compressibility of all the base oils, decreases with pressure of up to 35% and 45% were observed. From the contact angle measurements, it was observed that base oils with a higher viscosity grade have a worse wetting. The greatest effect of pressure on the dynamic viscosity was obtained for the naphthenic mineral oil and the lowest effect for the polyalphaolefin oil. Paraffinic and naphthenic oils present the highest universal pressure–viscosity coefficients.
Experimental Study on Flexible Fiber Assisted Shear Thickening Polishing for Cutting Edge Preparation of Core Drill
Authors: Shao Lanying , Zhou Yu , Dai Yanfei , Lyu Binghai
Published: February 2023

To improve the cutting performance of the core drill, the flexible fiber assisted shear-thickening polishing (FF-STP) for cutting edge preparation was proposed to eliminate the microscopic defect and obtain proper radius of the cutting edge of the core drill. The flexible fiber was introduced into the shear-thickening polishing process to break the thickened agglomerates and improve the efficiency of cutting edge preparation. The influence of the polishing speed, abrasive concentration and the flexible fiber contact length with the core drill on the cutting edge radius r and surface morphology of the core drill edge was analyzed, and the drilling experiments were carried out after preparation, the cutting heat and drilled holes’ roughness were employed as evaluation indexes to evaluate the performance of the core drill. The results show that the cutting edge radius increases with the increase of polishing speed, abrasive concentration and contact length. However, too high a polishing speed and contact length reduce the abrasive particles involved in the polishing process, and then lead to a decline in preparation efficiency. Under the selected processing conditions, the cutting edge radius increases from the initial 5 μm to 14 μm and 27 μm with 4 min of treatment and 6 min of treatment preparation, respectively. The sharp cutting edge becomes rounded, the burrs and chipping on the cutting edge are eliminated, and the average roughness (Ra) of the flank face decreases from 110.4 ± 10 nm to 8.0 ± 3 nm. Nine holes were drilled consecutively by core drills after cutting edge preparation, and the cutting temperature and drilled holes’ roughness were recorded. The maximum cutting temperature (122.4 °C) in the process with the prepared core drill (radius r = 14 μm) is about 20 °C lower than that with untreated one, and the roughness of the drilled hole (Ra 510.5 nm) about 189.9 nm lower. The results indicates that FF-STP is a promising method for high consistency preparation of the core drill cutting edge.
Synthesis and Characterization of New Potential Hypoxia-Sensitive Azo-thiacalix[4]arenes Derivatives
Authors: Gabdrakhmanova Farida B. , Churbanova Ekaterina S. , Khalifa Mohamed A. , Kleshnina Sofia R. , Solovieva Svetlana E. , Antipin Igor S.
Published: February 2023

The subject of this article is new potential hypoxia-sensitive azo-thiacalix[4]arenes derivatives in the 1,3-alternate configuration. Previously, it was shown that azo derivatives of calix[4]arene in the cone conformation form complexes with rhodamine dyes. The present work is devoted to the synthesis of new azo derivatives using the thiacalix[4]arene platform. A new highly productive method for the synthesis of thiacalixarene with four anionic sulfonate azo fragments on the lower rim (compounds 2a–b) for further complexation with the most common cationic dyes is reported. The chemical structures of the products obtained were established based on 1H and 13C NMR, IR spectroscopy, MALDI TOF mass spectrometry, and elemental analysis.