bluuebunny/crossref_metadata_2025 huggingface dataset:
Inertial Modification Using Self-Adaptive Subgradient Extragradient Techniques for Equilibrium Programming Applied to Variational Inequalities and Fixed-Point Problems
Authors: Rehman Habib ur , Kumam Wiyada , Sombut Kamonrat
Published: May 2022

Equilibrium problems are articulated in a variety of mathematical computing applications, including minimax and numerical programming, saddle-point problems, fixed-point problems, and variational inequalities. In this paper, we introduce improved iterative techniques for evaluating the numerical solution of an equilibrium problem in a Hilbert space with a pseudomonotone and a Lipschitz-type bifunction. These techniques are based on two computing steps of a proximal-like mapping with inertial terms. We investigated two simplified stepsize rules that do not require a line search, allowing the technique to be carried out more successfully without knowledge of the Lipschitz-type constant of the cost bifunction. Once control parameter constraints are put in place, the iterative sequences converge on a particular solution to the problem. We prove strong convergence theorems without knowing the Lipschitz-type bifunction constants. A sequence of numerical tests was performed, and the results confirmed the correctness and speedy convergence of the new techniques over the traditional ones.
On the Existence and Uniqueness of an Rν-Generalized Solution to the Stokes Problem with Corner Singularity
Authors: Rukavishnikov Viktor A. , Rukavishnikov Alexey V.
Published: May 2022

We consider the Stokes problem with the homogeneous Dirichlet boundary condition in a polygonal domain with one re-entrant corner on its boundary. We define an Rν-generalized solution of the problem in a nonsymmetric variational formulation. Such defined solution allows us to construct numerical methods for finding an approximate solution without loss of accuracy. In the paper, the existence and uniqueness of an Rν-generalized solution in weighted sets is proved.
Accounting Games: Using Matrix Algebra in Creating the Accounting Models
Authors: Vysotskaya Anna
Published: May 2022

The aim of this paper is to show the mathematical basis for a precise treatment of double-entry bookkeeping, which was first developed in the nineteenth century by Sir William Rowan Hamilton. This is done by using basic notions of matrix algebra founded on the idea of ordered pairs. We also reveal how complex numbers and rationals (fractions) developed in mainstream accounting science and became a leading platform for the ongoing processes within Industry 4.0. The paper concludes with examples of how accounting operations can be represented by matrix equations with the result of generating a final report. The author presents a mathematical model of accounting which is independent of specific existential forms, but which is capable of undertaking the form of any of them and thus which has the potential of being understood and accepted by specialists globally.
Five-Year Analysis of Surgical Site Infections in Three Orthopaedics and Trauma Wards under HAI-Net from the South of Poland in 2014–2018 Considering the Standardized Infection Ratio
Authors: Wałaszek Marta Zofia , Słowik Roża , Domański Adam , Wałaszek Michał Jan , Różańska Anna , Kołpa Małgorzata
Published: May 2022

Background and Objectives: Surgical site infections (SSIs) are the most common healthcare-associated infections (HAIs) in surgical wards. The highest risk of developing SSI is carried by operations involving implants, such as: hip prosthesis (HPRO), knee prosthesis (KPRO), open reduction of fracture (FX), and closed reduction of fracture with internal fixation (CR). Objectives. The objective of the study was to assess the incidence of SSI in patients subjected to HPRO, KPRO, FX, and CR procedures in orthopaedics and trauma wards in 2014–2018 considering risk factors included in the SIR index. Materials and Methods: The study included 6261 patients who were subjected to orthopaedic surgery in 2014–2018. The investigation covered three hospitals with orthopaedics and trauma wards. The research was conducted in the framework of the national HAI surveillance programme according to the methodology of the HAI-Net, ECDC. Results: A total of 6261 surgeries were investigated, of which 111 cases of SSI were detected. The incidence was 1.8%; HPRO (incidence 2.1%, median (Me) surgery duration 90 min, and standardized infection ratio (SIR) above 1 in all units tested); KPRO (incidence 2.0%, Me 103 min, and SIR above 1 for all units tested); FX (incidence 1.9%, Me 70 min, and SIR above 1 for two units tested and below 1 in one unit); CR (incidence 1.0%, Me 55 min, and SIR—not calculated). The etiological agents that were most frequently isolated from patients with SSI were Staphylococcus aureus, coagulase-negative Staphylococcus, and Klebsiella pneumoniae. Conclusions: HPRO, KPRO, and FX operations performed in the studied wards carried a higher risk of developing SSI than that predicted by SIR. SSIs accounted for a significant percentage of the overall infection pool in CR surgeries. Actions should be undertaken to reduce the incidence of SSI in these surgeries. There should be a hospital network which facilitates cooperation in order to better monitor and analyse the incidence of SSI.
Surface Optimization of Commercial Porous Ti Substrates by EPD of Titanium Nitride
Authors: Mortalò Cecilia , Cannio Maria , Zin Valentina , Miorin Enrico , Montagner Francesco , Pasquali Luca , Montecchi Monica , Boccaccini Dino Norberto , Fabrizio Monica , Deambrosis Silvia Maria
Published: May 2022

In this work, the infiltration of TiN powders by electrophoretic deposition (EPD) in aqueous media was considered as alternative method to reduce the size craters and the roughness of commercial porous Ti substrates. Ti substrates can be used as suitable supports for the deposition of dense hydrogen separation TiNx-based membranes by physical vapor deposition (PVD) techniques. The influence of various EPD deposition parameters on surface morphology and roughness of TiN-infiltrated substrates were investigated in order to optimize their surface properties. The results suggest that a multi-step EPD procedure is an effective technique for reducing substrate surface defects of commercial porous Ti substrates which could then be successfully used as proper supports for the deposition of dense and defect-free TiNx layers, also aligning the thermal mismatch between the active layer and the porous substrate.
Role of Lipoproteins in the Pathophysiology of Breast Cancer
Authors: Pandrangi Santhi Latha , Chittineedi Prasanthi , Chikati Rajasekhar , Mosquera Juan Alejandro Neira , Llaguno Sungey Naynee Sánchez , Mohiddin Gooty Jaffer , Lanka Suseela , Chalumuri Sphoorthi Shree , Maddu Narendra
Published: May 2022

Breast cancer is one of the most common malignancies in women and the leading cause of cancer mortality. Hypercholesterolemia and obesity are potential risk factors for the incidence of breast cancer, and their detection can enhance cancer prevention. In this paper, we discuss the current state of investigations on the importance of lipoproteins, such as low denisity lipoproteins (LDL) and high density lipoproteins (HDL), and cholesterol transporters in the progression of breast cancer, and the therapeutic strategies to reduce breast cancer mortality. Although some research has been unsuccessful at uncovering links between the roles of lipoproteins and breast cancer risk, major scientific trials have found a straight link between LDL levels and incidence of breast cancer, and an inverse link was found between HDL and breast cancer development. Cholesterol and its transporters were shown to have significant importance in the development of breast cancer in studies on breast cancer cell lines and experimental mice models. Instead of cholesterol, 27-hydroxycholesterol, which is a cholesterol metabolite, is thought to promote propagation and metastasis of estrogen receptor-positive breast cancer cell lines. Alteration of lipoproteins via oxidation and HDL glycation are thought to activate many pathways associated with inflammation, thereby promoting cellular proliferation and migration, leading to metastasis while suppressing apoptosis. Medications that lower cholesterol levels and apolipoprotein A-I mimics have appeared to be possible therapeutic agents for preventing excessive cholesterol’s role in promoting the development of breast cancer.
Silver/Snail Mucous PVA Nanofibers: Electrospun Synthesis and Antibacterial and Wound Healing Activities
Authors: El-Attar Aalaa A. , El-Wakil Hamdy B. , Hassanin Ahmed H. , Bakr Basant A. , Almutairi Tahani M. , Hagar Mohamed , Elwakil Bassma H. , Olama Zakia A.
Published: May 2022

Healthcare textiles are gaining great attention in the textile industry. Electrospun nanofibers are considered the golden soldiers due to their strength, flexibility, and eco-friendly properties. The present study aimed to evaluate the potency of polyvinyl alcohol (PVA) nanofibers loaded with newly biosynthesized silver nanoparticles (Ag-NPs) as a wound healing dressing. Chocolate-band snail (Eobania vermiculata) mucus (which is part of the Mollusca defense system) was used as a novel reducing and stabilizing agent. Data indicated the effectiveness of Eobania vermiculata’s mucus in silver nanoparticle synthesis after a 24 h incubation time. The biosynthesized AgNPs-SM showed a 13.15 nm particle size, −22.5 mV ζ potential, and 0.37 PDI, which proved the stability of the synthesized nanoparticles. Eobania vermiculata mucus and AgNPs-SM showed potent antibacterial activity, especially against Pseudomonas aeruginosa. The electrospinning technique was applied in the fabrication of PVA/AgNPs-SM nanofibers, which were homogenous with a fine diameter of about 100–170 nm and showed a significantly high antimicrobial activity. In vitro and in vivo studies revealed that PVA/AgNPs-SM nanofibers were safe and efficiently enhanced the wound healing process (typical histological picture of the proliferative phase with compact and well aligned collagen fibers in the dermal tissue after 12 days) together with bacterial growth inhibition in the infected skin area.
Effect of Water Vapor on the Microstructure of Al2O3 on the Free-Standing MCrAlY Alloy at 1100 °C
Authors: Wu Minxuan , Zheng Biju , Zhang Defeng , Huang Taihong , Li Chao , Li Qing , Huang Wenlang , Zhang Luyang , Song Peng
Published: May 2022

The oxidation resistance of the MCrAlY binding coat is due to the formation of protective Al2O3 oxide scale at high temperature. The oxidation behavior of NiCrAlYHf alloy in 1100 °C air and air-water vapor atmosphere was studied. The effect of water vapor on the microstructure and distribution of reactive elements was discussed. The results showed that the oxide scale in air has a double layer structure composed of columnar and equiaxed crystals, while the oxide scale in water vapor contains fine alumina grains, which provides more channels for the diffusion of reactive elements. In addition, The Cr element in the oxide scale is mainly concentrated in the outer equiaxed crystal zone, and the Hf oxide is mainly concentrated in the columnar crystal boundary. In air-water vapor atmosphere, the Cr element is uniformly distributed in the oxide scale.
Evolution of Microstructure and Hardness of the Nitrided Zone during Plasma Nitriding of High-Alloy Tool Steel
Authors: Landgraf Pierre , Bergelt Tim , Rymer Lisa-Marie , Kipp Christian , Grund Thomas , Bräuer Günter , Lampke Thomas
Published: May 2022

Plasma nitriding is widely used in various industrial applications to improve surface hardness and wear properties. Especially for tool steels, it is also used to improve the support and adhesion of diamond-like carbon (DLC) coatings. The properties of the nitrided zone produced by plasma nitriding are influenced by the applied process parameter, in particular temperature and time. However, for high-alloy tool steels, a deeper understanding of the underlying diffusion processes of the nitrogen and the interaction with the existing microstructure, as well as the effects on the case depth is still lacking. Therefore, in this study, specimens of high-alloy tool steel X153CrMoV12 were plasma nitrided at varying temperatures (480 °C, 520 °C, 560 °C) and treatment times (2 h, 4 h, 16 h). The resulting nitrided zones were investigated by optical and scanning electron microscopy (OM and SEM), depth-dependent glow discharge optical emission spectroscopy (GDOES), X-ray diffraction (XRD), and hardness measurements to characterize their microstructure, chemical composition, and hardness depending on the process parameters. The distribution of carbides (M7C3), e.g., chromium carbides, affects the diffusion of the nitrogen and the layer growth. An increase of temperature and duration leads to an increased layer thickness. The composition of the compound layer is, e.g., influenced by the process parameters: ε nitrides (Fe2–3N) occurred preferentially at lower temperatures, while γ′ nitrides (Fe4N) appeared mostly at higher temperatures. In order to investigate the influence of the carbides of the high-alloy tool steel on the nitriding process, a new methodology was developed by means of finite element analysis (FE), which makes it possible to analyze this influence on the development of the nitrogen concentration profile. This methodology makes it possible for the first time to map the heterogeneous nitrogen evolution and distribution.
Hydrogen Degassing of Zirconium under High-Vacuum Conditions
Authors: Fagnoni Francesco , Konarski Piotr
Published: May 2022

Micromechanics techniques, such as nano-indentation and micro-pillar compression, can be applied to study hydrogen-charged zirconium alloys at elevated temperatures, which is highly relevant for the nuclear industry. Such experiments are often conducted inside a scanning electron microscope (SEM) under high-vacuum conditions (10−5 mbar). The combination of a high-temperature and high-vacuum environment causes some hydrogen to escape from the sample into the chamber. Although this effect is evident at temperatures above 600 °C, the extent of hydrogen desorption at lower temperatures is still unclear. In the presented study, the desorption of hydrogen was assessed in zirconium cladding tube material under temperature and hydrogen content conditions comparable to those faced by used nuclear fuel during dry storage. The measured hydrogen loss due to the high vacuum was compared to the simulations obtained using an extended version of a hydrogen behavior tool developed at PSI.
Identification of Requirements for FE Modeling of an Adaptive Joining Technology Employing Friction-Spun Joint Connectors (FSJC)
Authors: Oesterwinter Annika , Wischer Christian , Homberg Werner
Published: May 2022

The adaptive joining process employing friction-spun joint connectors (FSJC) is a promising method for the realization of adaptable joints and thus for lightweight construction. In addition to experimental investigations, numerical studies are indispensable tools for its development. Therefore, this paper includes an analysis of boundary conditions for the spatial discretization and mesh modeling techniques, the material modeling, the contact and friction modeling, and the thermal boundary conditions for the finite element (FE) modeling of this joining process. For these investigations, two FE models corresponding to the two process steps were set up and compared with the two related processes of friction stir welding and friction drilling. Regarding the spatial discretization, the Lagrangian approach is not sufficient to represent the deformation that occurs. The Johnson-Cook model is well suited as a material model. The modeling of the contact detection and friction are important research subjects. Coulomb’s law of friction is not adequate to account for the complex friction phenomena of the adaptive joining process. The thermal boundary conditions play a decisive role in heat generation and thus in the material flow of the process. It is advisable to use temperature-dependent parameters and to investigate in detail the influence of radiation in the entire process.
In-Situ Thermography Investigation of Crack Growth in Armco Iron under Gigacycle Fatigue Loading
Authors: Postel Victor , Petit Johann , Wang Chong , Tan Kai , Ranc-Darbord Isabelle , Wang Qingyuan , Wagner Daniele
Published: May 2022

A non-destructive thermographic methodology based on the temperature field is utilized to determine the crack tip position during the very high cycle fatigue (VHCF) test of pure iron and deduce the corresponding fatigue crack growth rate (FCGR). To this end, a piezoelectric fatigue machine is employed to test 1 mm thick pure iron samples at 20 kHz in push–pull fatigue loading. Two cameras are placed on each side of the plate sample, an infrared one for measuring the temperature fields on the specimen surface and an optical one for visualizing the crack tip verification. The centre section of the specimen is notched to initiate the crack. The temperature field is converted into intrinsic dissipation to quantify the inelastic strain energy according to energy conservation. The maximum value of intrinsic dissipation in each thermal image is related to the position of the crack tip and thus allows monitoring of the crack evolution during the fatigue test. The obtained results show that one specific specimen broke at 7.25 × 107 cycles in the presence of a very low-stress amplitude (122 MPa). It is observed that the intrinsic dissipation has a low-constant level during the initiation and the short cracking, then sharply grows during the long cracking. This transition is visible on the polished surface of the sample, where the plasticity appears during the long cracking and slightly before. The material parameters in the Paris equation obtained from the intrinsic dissipation in the short crack growth are close to the results available in the literature as well as those obtained by the optical camera.
PET/MRI-Evaluated Activation of Brown Adipose Tissue via Cold Exposure Impacts Lipid Metabolism
Authors: Miniewska Katarzyna , Maliszewska Katarzyna , Pietrowska Karolina , Godzień Joanna , Łabieniec Łukasz , Mojsak Małgorzata , Krętowski Adam , Ciborowski Michał
Published: May 2022

Although brown adipose tissue (BAT) is considered to play a protective role against obesity and type 2 diabetes, the mechanisms of its activation and associations with clinical parameters are not well described. Male adults underwent a 2 h cold exposure (CE) to activate BAT and, based on the results of PET/MRI performed after the CE, were divided into BAT(+) and BAT(−) groups. During the CE procedure, blood samples were collected and alterations in plasma metabolome in both groups were investigated using LC-MS. Additionally, associations between clinical factors and BAT were examined. Moreover, levels of glucose, insulin, leptin, TNF-α, FGF21, and FABP4 were assessed in serum samples. In the BAT(+) group, levels of LPC(17:0), LPE(20:4), LPE(22:4), LPE(22:6), DHA, linoleic acid, and oleic acid increased during CE, whereas levels of sphinganine-phosphate and sphingosine-1-phosphate decreased. Levels of LPE(O-18:0), 9-HpODE, and oleic acid were elevated, while the level of LPE(20:5) was reduced in BAT(+) compared to BAT(−) subjects. AUCs of LPC(18:2), LPC(O-18:2)/LPC(P-18:1), and SM(d32:2) negatively correlated with BAT. In the BAT(+) group, the concentration of FABP4 during and after CE was decreased compared to the basal level. No alterations were observed in the BAT(−) group. In conclusion, using untargeted metabolomics, we proved that the plasma metabolome is affected by cold-induced BAT activation.
Metabolomics in Bariatric and Metabolic Surgery Research and the Potential of Deep Learning in Bridging the Gap
Authors: Pantelis Athanasios G.
Published: May 2022

During the past several years, there has been a shift in terminology from bariatric surgery alone to bariatric and metabolic surgery (BMS). More than a change in name, this signifies a paradigm shift that incorporates the metabolic effects of operations performed for weight loss and the amelioration of related medical problems. Metabolomics is a relatively novel concept in the field of bariatrics, with some consistent changes in metabolite concentrations before and after weight loss. However, the abundance of metabolites is not easy to handle. This is where artificial intelligence, and more specifically deep learning, would aid in revealing hidden relationships and would help the clinician in the decision-making process of patient selection in an individualized way.
Glutamate Efflux across the Blood–Brain Barrier: New Perspectives on the Relationship between Depression and the Glutamatergic System
Authors: Gruenbaum Benjamin Fredrick , Zlotnik Alexander , Frenkel Amit , Fleidervish Ilya , Boyko Matthew
Published: May 2022

Depression is a significant cause of disability and affects millions worldwide; however, antidepressant therapies often fail or are inadequate. Current medications for treating major depressive disorder can take weeks or months to reach efficacy, have troubling side effects, and are limited in their long-term capabilities. Recent studies have identified a new set of glutamate-based approaches, such as blood glutamate scavengers, which have the potential to provide alternatives to traditional antidepressants. In this review, we hypothesize as to the involvement of the glutamate system in the development of depression. We identify the mechanisms underlying glutamate dysregulation, offering new perspectives on the therapeutic modalities of depression with a focus on its relationship to blood–brain barrier (BBB) permeability. Ultimately, we conclude that in diseases with impaired BBB permeability, such as depression following stroke or traumatic brain injury, or in neurogenerative diseases, the glutamate system should be considered as a pathway to treatment. We propose that drugs such as blood glutamate scavengers should be further studied for treatment of these conditions.
The Lipid Energy Model: Reimagining Lipoprotein Function in the Context of Carbohydrate-Restricted Diets
Authors: Norwitz Nicholas G. , Soto-Mota Adrian , Kaplan Bob , Ludwig David S. , Budoff Matthew , Kontush Anatol , Feldman David
Published: May 2022

When lean people adopt carbohydrate-restricted diets (CRDs), they may develop a lipid profile consisting of elevated LDL-cholesterol (LDL-C) and HDL-cholesterol (HDL-C) with low triglycerides (TGs). The magnitude of this lipid profile correlates with BMI such that those with lower BMI exhibit larger increases in both LDL-C and HDL-C. The inverse association between BMI and LDL-C and HDL-C change on CRD contributed to the discovery of a subset of individuals—termed Lean Mass Hyper-Responders (LMHR)—who, despite normal pre-diet LDL-C, as compared to non-LMHR (mean levels of 148 and 145 mg/dL, respectively), exhibited a pronounced hyperlipidemic response to a CRD, with mean LDL-C and HDL-C levels increasing to 320 and 99 mg/dL, respectively, in the context of mean TG of 47 mg/dL. In some LMHR, LDL-C levels may be in excess of 500 mg/dL, again, with relatively normal pre-diet LDL-C and absent of genetic findings indicative of familial hypercholesterolemia in those who have been tested. The Lipid Energy Model (LEM) attempts to explain this metabolic phenomenon by positing that, with carbohydrate restriction in lean persons, the increased dependence on fat as a metabolic substrate drives increased hepatic secretion and peripheral uptake of TG contained within very low-density lipoproteins (VLDL) by lipoprotein lipase, resulting in marked elevations of LDL-C and HDL-C, and low TG. Herein, we review the core features of the LEM. We review several existing lines of evidence supporting the model and suggest ways to test the model’s predictions.
The Legend of ATP: From Origin of Life to Precision Medicine
Authors: Chu Xin-Yi , Xu Yuan-Yuan , Tong Xin-Yu , Wang Gang , Zhang Hong-Yu
Published: May 2022

Adenosine triphosphate (ATP) may be the most important biological small molecule. Since it was discovered in 1929, ATP has been regarded as life’s energy reservoir. However, this compound means more to life. Its legend starts at the dawn of life and lasts to this day. ATP must be the basic component of ancient ribozymes and may facilitate the origin of structured proteins. In the existing organisms, ATP continues to construct ribonucleic acid (RNA) and work as a protein cofactor. ATP also functions as a biological hydrotrope, which may keep macromolecules soluble in the primitive environment and can regulate phase separation in modern cells. These functions are involved in the pathogenesis of aging-related diseases and breast cancer, providing clues to discovering anti-aging agents and precision medicine tactics for breast cancer.
Biological Control of Acinetobacter baumannii: In Vitro and In Vivo Activity, Limitations, and Combination Therapies
Authors: Havenga Benjamin , Reyneke Brandon , Waso-Reyneke Monique , Ndlovu Thando , Khan Sehaam , Khan Wesaal
Published: May 2022

The survival, proliferation, and epidemic spread of Acinetobacter baumannii (A. baumannii) in hospital settings is associated with several characteristics, including resistance to many commercially available antibiotics as well as the expression of multiple virulence mechanisms. This severely limits therapeutic options, with increased mortality and morbidity rates recorded worldwide. The World Health Organisation, thus, recognises A. baumannii as one of the critical pathogens that need to be prioritised for the development of new antibiotics or treatment. The current review will thus provide a brief overview of the antibiotic resistance and virulence mechanisms associated with A. baumannii’s “persist and resist strategy”. Thereafter, the potential of biological control agents including secondary metabolites such as biosurfactants [lipopeptides (surfactin and serrawettin) and glycolipids (rhamnolipid)] as well as predatory bacteria (Bdellovibrio bacteriovorus) and bacteriophages to directly target A. baumannii, will be discussed in terms of their in vitro and in vivo activity. In addition, limitations and corresponding mitigations strategies will be outlined, including curtailing resistance development using combination therapies, product stabilisation, and large-scale (up-scaling) production.
The Identification and Characterization of Endopolygalacturonases in a South African Isolate of Phytophthora cinnamomi
Authors: Miyambo Tsakani Magdeline , Backer Robert , Engelbrecht Juanita , Joubert Fourie , van der Merwe Nicolaas Albertus , van den Berg Noëlani
Published: May 2022

Phytophthora cinnamomi is an economically important plant pathogen that has caused devastating losses to the avocado industry worldwide. To facilitate penetration and successful colonization of the host plant, pathogens have been reported to secrete polygalacturonases (PGs). Although a large PG gene family has been reported in P. cinnamomi, in-depth bioinformatics analyses and characterization of these genes is still lacking. In this study we used bioinformatics tools and molecular biology techniques to identify and characterize endopolygalacturonases in the genome of a South African P. cinnamomi isolate, GKB4. We identified 37 PGs, with 19 characteristics of full-length PGs. Although eight PcPGs were induced in planta during infection, only three showed significant up- and down-regulation when compared with in vitro mycelial growth, suggesting their possible roles in infection. The phylogenetic analysis of PcPGs showed both gain and loss of introns in the evolution of PGs in P. cinnamomi. Furthermore, 17 PGs were related to characterized PGs from oomycete species, providing insight on possible function. This study provides new data on endoPGs in P. cinnamomi and the evolution of introns in PcPG genes. We also provide a baseline for future functional characterization of PGs suspected to contribute to P. cinnamomi pathogenicity/virulence in avocado.
Vanadium: A Review of Different Extraction Methods to Evaluate Bioavailability and Speciation
Authors: Yang Jie , Wang Yunlong , Gao Xiaohui , Zuo Rui , Song Liuting , Jin Chenhui , Wang Jinsheng , Teng Yanguo
Published: May 2022

The excessive input of heavy metals such as vanadium (V) into the environment has been one of the consequences of global industrial development. Excessive exposure to V can pose a potential threat to ecological safety and human health. Due to the heterogeneous composition and reactivity of the various elements in soils and sediments, quantitative analysis of the chemical speciation of V in different environmental samples is very complicated. The analysis of V chemical speciation can further reveal the bioavailability of V and accurately quantify its ecotoxicity. This is essential for assessing for exposure and for controlling ecological risks of V. Although the current investigation technologies for the chemical speciation of V have grown rapidly, the lack of comprehensive comparisons and systematic analyses of these types of technologies impedes a more comprehensive understanding of ecosystem safety and human health risks. In this review, we studied the chemical and physical extraction methods for V from multiple perspectives, such as technological, principle-based, and efficiency-based, and their application to the evaluation of V bioavailability. By sorting out the advantages and disadvantages of the current technologies, the future demand for the in situ detection of trace heavy metals such as V can be met and the accuracy of heavy metal bioavailability prediction can be improved, which will be conducive to development in the fields of environmental protection policy and risk management.
The Gel Growth Technique—A Neglected Yet Effective Tool to Prepare Crystals of Oxysalts and Supergene Minerals
Authors: Talla Dominik , Wagner Andreas
Published: May 2022

The technique of crystal growth in gels has nowadays become somewhat neglected in the scope of earth sciences, to the disadvantage of the experimental mineralogist. Even preparing an inorganic silica gel can prove a challenge to many, let alone successfully configure the entire experiment. Based not only on previous literature but also on our extensive experience, crystals of many substances, including supergene minerals as reference standards, can be successfully grown in gel, aiding in accomplishing various research goals in earth sciences. Instead of providing the reader with an overwhelming compendium of historical information and theoretical knowledge of the subject which can be found elsewhere, we presented herein a comprehensive, practically oriented guide to the understanding and successful use of the technique of crystal growth in gels, mentioning, in addition to the general principle, the numerous pitfalls which we encountered during our own use of the method, and the ways to overcome them. Despite that the procedure is nowadays used mainly for the laboratory synthesis of organic or metal-organic compounds, we believe it to be a valuable asset to any mineralogist, and often, the only way to obtain inorganic reference material of a particular mineral of interest.
Assessment of Materials and Rare Earth Metals Demand for Sustainable Wind Energy Growth in India
Authors: Verma Shalini , Paul Akshoy Ranjan , Haque Nawshad
Published: May 2022

Wind energy is an alternative energy generation from non-renewable energy resources. The turbine is used to exploit wind energy. Different components of a wind turbine necessitate different materials and metals. There is a dependency of the materials on foreign countries. To avoid future problems regarding the availability of these materials in India, it is necessary to forecast the quantity and the price of the materials and metals. Thus, this study mainly focuses on the estimation of the raw materials, rare earth, and critical metals used in manufacturing the wind turbine. Two wind turbines of 1.65 MW and 3.45 MW capacity, 78 m and 94 m hub height are considered for the study. The major raw materials are steel, aluminum, copper, cast iron, fiber glass with epoxy resin, and ceramic/glass. The requirement of rare earth elements (Nd) depends on the type of wind turbine direct drive or geared, and the type of generator used in the direct-drive wind turbine. The results estimated the requirement of materials and rare earth elements and the expected price in the future for 100% wind energy production in India.
Anti-Obesity and Anti-Adipogenic Effects of Administration of Arginyl-Fructose-Enriched Jeju Barley (Hordeum vulgare L.) Extract in C57BL/6 Mice and in 3T3-L1 Preadipocytes Models
Authors: Lee Soo-Young , Kim Tae-Yang , Hong Ji-Yoon , Kim Gi-Jung , Oh Jung-Bae , Kim Min-Joo , Apostolidis Emmanouil , Lee Jung-Yun , Kwon Young-In
Published: May 2022

In our previous study, we reported that arginyl-fructose (AF), one of the Amadori rearrangement compounds (ARCs) produced by the heat processing of Korean ginseng can reduce carbohydrate absorption by inhibiting intestinal carbohydrate hydrolyzing enzymes in both in vitro and in vivo animal models. This reduced absorption of carbohydrate might be helpful to control body weight gain due to excessive carbohydrate consumption and support induced calorie restriction. However, the weight management effect, except for the effect due to anti-hyperglycemic action, along with the potential mechanism of action have not yet been determined. Therefore, the efforts of this study are to investigate and understand the possible weight management effect and mechanism action of AF-enriched barley extracts (BEE). More specifically, the effect of BEE on lipid accumulation and adipogenic gene expression, body weight gain, body weight, plasma lipids, body fat mass, and lipid deposition were evaluated using C57BL/6 mice and 3T3-L1 preadipocytes models. The formation of lipid droplets in the 3T3-L1 treated with BEE (500 and 750 µg/mL) was significantly blocked (p < 0.05 and p < 0.01, respectively). Male C57BL/6 mice were fed a high-fat diet (30% fat) for 8 weeks with BEE (0.3 g/kg-body weight). Compared to the high fat diet control (HFD) group, the cells treated with BEE significantly decreased in intracellular lipid accumulation with concomitant decreases in the expression of key transcription factors, peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein alpha (CEBP/α), the mRNA expression of downstream lipogenic target genes such as fatty acid binding protein 4 (FABP4), fatty acid synthase (FAS), and sterol regulatory element-binding protein 1c (SREBP-1c). Supplementation of BEE effectively lowered the body weight gain, visceral fat accumulation, and plasma lipid concentrations. Compared to the HFD group, BEE significantly suppressed body weight gain (16.06 ± 2.44 g vs. 9.40 ± 1.39 g, p < 0.01) and increased serum adiponectin levels, significantly, 1.6-folder higher than the control group. These results indicate that AF-enriched barley extracts may prevent diet-induced weight gain and the anti-obesity effect is mediated in part by inhibiting adipogenesis and increasing adiponectin level.
Enhanced Toxicity of Bisphenols Together with UV Filters in Water: Identification of Synergy and Antagonism in Three-Component Mixtures
Authors: Kudłak Błażej , Jatkowska Natalia , Liu Wen , Williams Michael J. , Barcelo Damia , Schiöth Helgi B.
Published: May 2022

Contaminants of emerging concern (CEC) localize in the biome in variable combinations of complex mixtures that are often environmentally persistent, bioaccumulate and biomagnify, prompting a need for extensive monitoring. Many cosmetics include UV filters that are listed as CECs, such as benzophenone derivatives (oxybenzone, OXYB), cinnamates (2-ethylhexyl 4-methoxycinnamate, EMC) and camphor derivatives (4-methylbenzylidene-camphor, 4MBC). Furthermore, in numerous water sources, these UV filters have been detected together with Bisphenols (BPs), which are commonly used in plastics and can be physiologically detrimental. We utilized bioluminescent bacteria (Microtox assay) to monitor these CEC mixtures at environmentally relevant doses, and performed the first systematic study involving three sunscreen components (OXYB, 4MBC and EMC) and three BPs (BPA, BPS or BPF). Moreover, a breast cell line and cell viability assay were employed to determine the possible effect of these mixtures on human cells. Toxicity modeling, with concentration addition (CA) and independent action (IA) approaches, was performed, followed by data interpretation using Model Deviation Ratio (MDR) evaluation. The results show that UV filter sunscreen constituents and BPs interact at environmentally relevant concentrations. Of notable interest, mixtures containing any pair of three BPs (e.g., BPA + BPS, BPA + BPF and BPS + BPF), together with one sunscreen component (OXYB, 4MBC or EMC), showed strong synergy or overadditive effects. On the other hand, mixtures containing two UV filters (any pair of OXYB, 4MBC and EMC) and one BP (BPA, BPS or BPF) had a strong propensity towards concentration dependent underestimation. The three-component mixtures of UV filters (4MBC, EMC and OXYB) acted in an antagonistic manner toward each other, which was confirmed using a human cell line model. This study is one of the most comprehensive involving sunscreen constituents and BPs in complex mixtures, and provides new insights into potentially important interactions between these compounds.
Quercetin Reduces Inflammation and Protects Gut Microbiota in Broilers
Authors: Sun Lei , Guo Lewei , Xu Gaoqing , Li Zhiqiang , Appiah Michael Osei , Yang Lianyu , Lu Wenfa
Published: May 2022

The aim of this study was to investigate the effects of quercetin on inflammatory response and intestinal microflora in broiler chicken jejuna. A total of 120 broiler chickens were allocated into 3 groups: saline-challenged broilers fed a basal diet (CTR group), lipopolysaccharide (LPS)-challenged broilers fed a basal diet (L group) and LPS-challenged broilers fed a basal diet supplemented with 200 mg/kg quercetin (LQ group). Our results showed that LPS significantly increased expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-8, interferon (IFN)-γ, toll-like receptor (TLR)-4, Bax, Caspase-3 and diamine oxidase activity (DAO), and decreased expression of zona occludens-1 (ZO-1), Occludin and Bcl-2 in the jejunum, while dietary quercetin prevented the adverse effects of LPS injection. LPS injection significantly decreased the number of Actinobacteria, Armatimonadetes and Fibrobacteriae at the phylum level when compared to the CTR group. Additionally, at genus level, compared with the CTR group, the abundance of Halomonas, Micromonospora, Nitriliruptor, Peptococcus, Rubellimicrobium, Rubrobacter and Slaclda in L group was significantly decreased, while dietary quercetin restored the numbers of these bacteria. In conclusion, our results demonstrated that dietary quercetin could alleviate inflammatory responses of broiler chickens accompanied by modulating jejunum microflora.
New Potential Pharmacological Targets of Plant-Derived Hydroxyanthraquinones from Rubia spp.
Authors: Alov Petko , Al Sharif Merilin , Najdenski Hristo , Pencheva Tania , Tsakovska Ivanka , Zaharieva Maya Margaritova , Pajeva Ilza
Published: May 2022

The increased use of polyphenols nowadays poses the need for identification of their new pharmacological targets. Recently, structure similarity-based virtual screening of DrugBank outlined pseudopurpurin, a hydroxyanthraquinone from Rubia cordifolia spp., as similar to gatifloxacin, a synthetic antibacterial agent. This suggested the bacterial DNA gyrase and DNA topoisomerase IV as potential pharmacological targets of pseudopurpurin. In this study, estimation of structural similarity to referent antibacterial agents and molecular docking in the DNA gyrase and DNA topoisomerase IV complexes were performed for a homologous series of four hydroxyanthraquinones. Estimation of shape- and chemical feature-based similarity with (S)-gatifloxacin, a DNA gyrase inhibitor, and (S)-levofloxacin, a DNA topoisomerase IV inhibitor, outlined pseudopurpurin and munjistin as the most similar structures. The docking simulations supported the hypothesis for a plausible antibacterial activity of hydroxyanthraquinones. The predicted docking poses were grouped into 13 binding modes based on spatial similarities in the active site. The simultaneous presence of 1-OH and 3-COOH substituents in the anthraquinone scaffold were emphasized as relevant features for the binding modes’ variability and ability of the compounds to strongly bind in the DNA-enzyme complexes. The results reveal new potential pharmacological targets of the studied polyphenols and help in their prioritization as drug candidates and dietary supplements.
In Search of Synergistic Insect Repellents: Modeling of Muscarinic GPCR Interactions with Classical and Bitopic Photoactive Ligands
Authors: Niklas Beata , Lapied Bruno , Nowak Wieslaw
Published: May 2022

Insect vector-borne diseases pose serious health problems, so there is a high demand for efficient molecules that could reduce transmission. Using molecular docking and molecular dynamics (MD) simulation, we studied a series of compounds acting on human and insect muscarinic acetylcholine receptors (mAChRs), a novel target of synergistic agents in pest control. We characterized early conformational changes of human M1 and fruit fly type-A mAChR G protein-coupled receptors (GPCRs) in response to DEET, IR3535, and muscarine binding based on the MD analysis of the activation microswitches known to form the signal transduction pathway in class A GPCRs. We indicated groups of microswitches that are the most affected by the presence of a ligand. Moreover, to increase selectivity towards insects, we proposed a new, bitopic, photoswitchable mAChR ligand—BQCA-azo-IR353 and studied its interactions with both receptors. Modeling data showed that using a bitopic ligand may be a promising strategy in the search for better insect control.
Aging Process Effects on the Characteristics of Vacuum Residue Oxidation Products with the Addition of Crumb Rubber
Authors: Ongarbayev Yerdos , Zhambolova Ainur , Tileuberdi Yerbol , Mansurov Zulkhair , Oliviero Rossi Cesare , Calandra Pietro , Teltayev Bagdat
Published: May 2022

This paper considers the effect of aging processes on viscoelastic characteristics of vacuum residue oxidation products modified with crumb rubber. Viscoelastic properties were compared to original bitumen raw material-vacuum residue and vacuum residue oxidation products during short-term and long-term aging. The complex shear modulus of the vacuum residue and its oxidation products decreased with an increase in temperature. Short-term aging resulted in increased shear modulus for all samples.The vacuum residue oxidation product modified with crumb rubber had the maximum values of the rutting parameter and fatigue parameter. There was an expansion of the temperature range of plasticity: for the vacuum residue oxidation product with crumb rubber, its value was 67.2 °C. The curves of the black diagram of the modified vacuum residue oxidation product are shifted towards smaller phase angles with the increase in the shear modulus, which indicates the increase in the stiffness and elasticity of the rubber bitumen binders. The vacuum residue oxidation product modified with crumb rubber corresponded to the rubber bitumen binder of the grade RBB 60/90, according to its physical and mechanical indicators.
Chemical Compounds of Berry-Derived Polyphenols and Their Effects on Gut Microbiota, Inflammation, and Cancer
Authors: Bouyahya Abdelhakim , Omari Nasreddine El , EL Hachlafi Naoufal , Jemly Meryem El , Hakkour Maryam , Balahbib Abdelaali , El Menyiy Naoual , Bakrim Saad , Naceiri Mrabti Hanae , Khouchlaa Aya , Mahomoodally Mohamad Fawzi , Catauro Michelina , Montesano Domenico , Zengin Gokhan
Published: May 2022

Berry-derived polyphenols are bioactive compounds synthesized and secreted by several berry fruits. These polyphenols feature a diversity of chemical compounds, including phenolic acids and flavonoids. Here, we report the beneficial health effects of berry-derived polyphenols and their therapeutical application on gut-microbiota-related diseases, including inflammation and cancer. Pharmacokinetic investigations have confirmed the absorption, availability, and metabolism of berry-derived polyphenols. In vitro and in vivo tests, as well as clinical trials, showed that berry-derived polyphenols can positively modulate the gut microbiota, inhibiting inflammation and cancer development. Indeed, these compounds inhibit the growth of pathogenic bacteria and also promote beneficial bacteria. Moreover, berry-derived polyphenols exhibit therapeutic effects against different gut-microbiota-related disorders such as inflammation, cancer, and metabolic disorders. Moreover, these polyphenols can manage the inflammation via various mechanisms, in particular the inhibition of the transcriptional factor Nf-κB. Berry-derived polyphenols have also shown remarkable effects on different types of cancer, including colorectal, breast, esophageal, and prostate cancer. Moreover, certain metabolic disorders such as diabetes and atherosclerosis were also managed by berry-derived polyphenols through different mechanisms. These data showed that polyphenols from berries are a promising source of bioactive compounds capable of modulating the intestinal microbiota, and therefore managing cancer and associated metabolic diseases. However, further investigations should be carried out to determine the mechanisms of action of berry-derived polyphenol bioactive compounds to validate their safety and examinate their clinical uses.
Production and Analysis of the Physicochemical Properties of the Pyrolytic Oil Obtained from Pyrolysis of Different Thermoplastics and Plastic Mixtures
Authors: Palmay Paul , Haro Carla , Huacho Iván , Barzallo Diego , Bruno Joan Carles
Published: May 2022

The constant search for the proper management of non-degradable waste in conjunction with the circular economy makes the thermal pyrolysis of plastics an important technique for obtaining products with industrial interest. The present study aims to produce pyrolytic oil from thermoplastics and their different mixtures in order to determine the best performance between these and different mixtures, as well as to characterize the liquid fraction obtained to analyze its use based on said properties. This was carried out in a batch type reactor at a temperature of 400 °C for both individual plastics and their mixtures, from which the yields of the different fractions are obtained. The liquid fraction of interest is characterized by gas chromatography and its properties are characterized by ASTM standards. The product of the pyrolysis of mixtures of 75% polystyrene and 25% polypropylene presents a yield of 82%, being the highest, with a viscosity of 1.12 cSt and a calorific power of 42.5 MJ/kg, which has a composition of compounds of carbon chains ranging between C6 and C20, for which it is proposed as a good additive agent to conventional fuels for industrial use.