bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantifying adiabatic motion in the outer radiation belt and ring current with invariant matching
Authors: da Silva D. E. , Elkington S. R. , Li X. , Hudson M. K.
Published: May 2024

Adiabatic motion is a fundamental reversible process for geomagnetically trapped particle populations, including particles comprising the ring current and radiation belts. During adiabatic motion, a particle’s trajectory in configuration space responds to sufficiently slow changes in the magnetospheric magnetic field. Previous research has highlighted expected patterns in adiabatic motion, such as radial motion or the Dst effect. In this work, we introduce a method we call Invariant Matching for quantifying adiabatic motion between a pair of magnetospheres. This method can be applied to both simulation and semi-empirical magnetic field models, is computationally efficient, and in particular does not require tracing the particle trajectories. In this work, we use the Tsyganenko et al., Journal of Geophysical Research: Space Physics, 2005, 110 (TS05) magnetic field model, and present adiabatic motion between a storm commencement, the time of the storm’s Dst minimum, and a nominal recovery time. We also analyze adiabatic motion which occurs in response to enhancements of individual major current systems (including the ring current, Chapman-Ferraro current, Birkeland current, and tail current). Our methodology yields vector fields quantifying the displacement of mirror points throughout the magnetosphere, prepared in a way appropriate for application to both outer radiation belt and ring current populations.
GOLD plasma bubble observations comparison with geolocation of plasma irregularities by back propagation of the high-rate FORMOSA7/COSMIC 2 scintillation data
Authors: Wu Qian , Braun John , Sokolovskiy Sergey , Schreiner William , Pedatella Nicholas , Weiss Jan-Peter , Cherniak Iurii , Zakharenkova Irina
Published: May 2024

Using the high-rate phase and amplitude scintillation data from FORMOSA7/COSMIC two mission and back-propagation method, we geolocate plasma irregularities that cause scintillations. The results of geolocation are compared with the NASA GOLD UV image data of plasma bubbles. The root mean square of the zonal difference between estimated locations of plasma irregularities and plasma bubbles are about 1.5° and for single intersection cases 0.5° in the magnetic longitude. The geolocation data provide more accurate scintillation location around the globe compared to assigning to the tangent point and is valuable space weather product, which will be routinely available for public use.
The complementarity of nutrient density and disease burden for Nutritional Life Cycle Assessment
Authors: Cardinaals Renée P. M. , Verly E. , Jolliet O. , Van Zanten H. H. E. , Huppertz T.
Published: May 2024

The triple burden of obesity, undernutrition and climate change calls for systemic action to find solutions that co-benefit human and planetary health. A Nutritional Life Cycle Assessment (nLCA) can be used as a tool to assess the health- and environmental impact of foods and guide a transition to healthy and sustainable diets. Thus far, nLCAs have used the nutrient content of foods to represent their health impact, whereas the disease risk linked to under- or overconsuming certain nutrients, foods or food groups has been largely underutilized. This study explored, for the Dutch diet, the correlation between an indicator for essential nutrient density and for the disease burden of individual food items, i.e., a Nutrient Rich Food index with 24 essential nutrients (NRF24) and the HEalth Nutritional Index (HENI), respectively. NRF24 and HENI scores were calculated for food items contained in the Dutch Food Composition database. A very weak correlation between NRF24 and HENI values confirmed that nutrient density and disease burden should be considered as complementary and thus, that a high nutrient density does not directly imply a low disease burden, and vice versa. Moreover, the direction and strength of the correlation was food group-dependent, with negative correlations between NRF24 and HENI mainly observed for animal-based products, with the exception of dairy. In addition, the correlations between the nutrition-based indicators and indicators for greenhouse gas emissions, land use and water use were mostly weak, which stresses the need to include health impact in traditional LCAs because foods with a high nutrient density and low disease burden can imply trade-offs due to high environmental impacts. We therefore conclude that multiple indicators for health and environmental impact should be considered side-by-side in nLCA to avoid the risk of missing important information and trade-offs when assessing the performance of individual food items for healthy and sustainable diets.
Laparoscopic simultaneous anterograde inguinal and pelvic lymphadenectomy for penile cancer: two planses, three holes, and six steps
Authors: Yi Xian-lin , Li Xiu-ning , Lu Yu-lei , Lu Hao-yuan , Chen Yu , Zeng Li-xia , Qin Wen , Wu Yun , Tang Yong
Published: May 2024

Objective

To assess the feasibility, safety, and efficiency of simultaneous anterograde video laparoscopic inguinal and pelvic lymphadenectomy for penile cancer.

Materials and methods

We reviewed retrospectively the records of 22 patients (44 lateral) who underwent inguinal lymph nodes dissection for penile cancer. The procedure was standardized as two planes, three holes, and six steps. Two Separate-planes: superior plane of eternal oblique aponeurosis/ / fascia lata; inferior plane of superficial camper fascia. Three holes: two artificial lateral boundary holes, the internal and external boundary holes, and the hole of oval fossa. Six steps: separate the first separate-plane; separate the second layer; separate two artificial lateral boundary holes; free great saphenous vein; separate the third hole and clean up the deep inguinal lymph nodes; pelvic lymphadenectomy.

Results

A total of 22 cases were included and 9 patients underwent simultaneous pelvic lymphadenectomy. The average operation time on both sides was 7.52 ± 3.29 h, which was 0.5–1 h/side after skilled. The average amount of bleeding was 93.18 ± 50.84 ml. A total of 8 patients had postoperative complications, accounting for 36.36%, and no complications great than Clavien-Dindo class III occurred.

Conclusion

This study demonstrated that the video laparoscopic simultaneous anterograde inguinal and pelvic lymphadenectomy is a feasible and safe technique. Indocyanine Green was helpful for lymph node identify.
New-onset postoperative atrial fibrillation after pulmonary endarterectomy is associated with adverse outcomes
Authors: Zhang Dingkai , Zhang Zhaohua , Zhen Yanan , Liu Xiaopeng , Fan Xueqiang , Ye Zhidong , Liu Peng
Published: May 2024

Background

New-onset postoperative atrial fibrillation (POAF) is a common complication after pulmonary thromboendarterectomy (PEA), yet the risk factors and their impact on prognosis remain poorly understood. This study aims to investigate the risk factors associated with new-onset POAF after PEA and elucidate its underlying connection with adverse postoperative outcomes.

Methods

A retrospective analysis included 129 consecutive chronic thromboembolic pulmonary hypertension (CTEPH) patients and 16 sarcoma patients undergoing PEA. Univariate and multivariate analyses were conducted to examine the potential effects of preoperative and intraoperative variables on new-onset POAF following PEA. Propensity score matching (PSM) was then employed to adjust for confounding factors.

Results

Binary logistic regression revealed that age (odds ratio [OR] = 1.041, 95% confidence interval [CI] = 1.008–1.075, p = 0.014) and left atrial diameter[LAD] (OR = 1.105, 95% CI = 1.025–1.191, p = 0.009) were independent risk factors for new-onset POAF after PEA. The receiver operating characteristic (ROC) curve indicated that the predictive abilities of age and LAD for new-onset POAF were 0.652 and 0.684, respectively. Patients with new-onset POAF, compared with those without, exhibited a higher incidence of adverse outcomes (in-hospital mortality, acute heart failure, acute kidney insufficiency, reperfusion pulmonary edema). Propensity score matching (PSM) analyses confirmed the results.

Conclusion

Advanced age and LAD independently contribute to the risk of new-onset POAF after PEA. Patients with new-onset POAF are more prone to adverse outcomes. Therefore, heightened vigilance and careful monitoring of POAF after PEA are warranted.
Comparison of the efficacy of ROI-C cage with Zero-P device in anterior cervical discectomy and fusion of cervical degenerative disc disease: a two-year follow-up study
Authors: Wu Penghuan , Yang Sifan , Wang Yu , Wu Qiang , Zhang Yingze
Published: May 2024

Background

This study aimed to compare the clinical outcomes of Zero-P and ROI-C devices applied to anterior cervical discectomy and fusion (ACDF) surgery of cervical degenerative disc disease (CDDD).

Methods

From January 2020 and December 2020, 56 patients with CDDD who underwent ACDF using Zero-P or ROI-C were included in this retrospective study. The outcomes included visual analogue scale (VAS) score, Japanese Orthopedic Association (JOA) score, neck disability index (NDI) score, Cobb angle, dysphagia, and bone fusion rate. Dysphagia was assessed using the Bazaz grading system. The comparison of outcomes between the two groups was based on the 2-year follow-up time point, which was defined as the last follow-up visit.

Results

The Zero-P group included 16 males and 14 females, with a mean age of 56.2 (range, 35–65) years. The ROI-C group included 11 males and 15 females, with a mean age of 57.4 (range, 36–67) years. There was no significant difference in gender and mean age between the two groups. There were no significant differences in VAS score, JOA score, NDI score, Cobb angle, dysphagia, and bone fusion rate between two groups at the last follow up visit. In the Zero-P group, the duration of surgeries involving C3–4 or C6–7 segments was significantly longer than those including C4–5 or C5–6 segments (135.0 ± 19.0 vs. 105.6 ± 17.5 min, P < 0.05). In surgeries involving C3–4 or C6–7 segments, the operation time of ROI-C was significantly shorter than that of Zero-P (106.5 ± 19.5 vs.112.2 ± 20.5 min, P < 0.05). There were no significant differences in the dysphagia or cage subsidence rates between the Zero-P and ROI-C groups (P > 0.05). The Cobb angle in the last follow-up visit in the Zero-P group (24.4 ± 4.5°) was significantly higher than that in the ROI-C group (18.1 ± 2.3°) (P < 0.05).

Conclusions

ACDF using ROI-C device showed an efficacy similar to the Zero-P device, as well as a shorter operation time for surgeries involving C3–4 or C6–7 segments. However, ROI-C could cause more loss of Cobb angle over time, which could lead to uncomfortable symptoms.
Does pyrethroid exposure lower human semen quality? a systematic review and meta-analysis
Authors: Akhigbe Roland Eghoghosoa , Oyedokun Precious Adeoye , Akhigbe Tunmise Maryanne , Adenike Suliat , Oladipo Ayoola Abimbola , Hughes Jennifer Rose
Published: May 2024

Background: Pyrethroids are natural organic compounds extracted from flowers of pyrethrums and commonly used as domestic and commercial insecticides. Although it is effective in insect and parasitic control, its associated toxicity, including spermotoxicity, remains a challenge globally. Currently, the available reports on the effect of pyrethroids on semen quality are conflicting, hence an evaluation of its detrimental effect is pertinent. This study conducts a detailed systematic review and meta-analysis of the effects of pyrethroids on sperm quality.

Materials and methods: The present study was performed according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Using a pre-defined strategic protocol, an internet search was done using combined text words. The criteria for eligibility were selected based on Population, Exposure, Comparator, Outcome, and Study Designs (PECO) framework, and relevant data were collected. Appraisal was done using The Office of Health Assessment and Translation (OHAT) tool for the evaluation of the Risk of Bias and the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) Working Group guidelines for the certainty of evidence. A quantitative meta-analysis was conducted with the Review Manager (RevMan).

Results: Only 12 out of the 4, 050 studies screened were eligible for inclusion in this study. The eligible studies were from China (4), Japan (3), Poland (3), and United States (2). All the eligible studies were cross-sectional. A total of 2, 050 male subjects were included in the meta-analysis. Pyrethroid exposure significantly reduced sperm motility. Region-stratified subgroup analyses revealed that pyrethroid significantly reduced sperm motility among men in Poland and United States, and decreased sperm count among men in Japan. Pyrethroid exposure also reduced sperm concentration among men in Poland but increased sperm concentration among men in the United States.

Conclusion: Although the study revealed inconsistent evidence on the detrimental effect of pyrethroids on semen quality, the findings showed that pyrethroids have deleterious potentials on sperm motility, count, and concentration. Studies focusing on the assessment of semen quality in pyrethroid-exposed men, especially at specific varying levels of exposure, and employing prospective cohort studies or controlled cross-sectional designs are recommended.
The regulatory mechanism of garlic skin improving the growth performance of fattening sheep through metabolism and immunity
Authors: Xu Yongjie , Yi Mingliang , Sun Shixin , Wang Lei , Zhang Zijun , Ling Yinghui , Cao Hongguo
Published: May 2024

Objective

Garlic skin (GAS) has been proven to improve the growth performance of fattening sheep. However, the mechanism by which GAS affects fattening sheep is not yet clear. The aim of this study is to investigate the effects of adding GAS to feed on the growth performance, rumen and fecal microbiota, serum and urine metabolism, and transcriptomics of rumen epithelial cells in fattening sheep.

Methods

GAS with 80 g/kg dry matter (DM) was added to the diet of fattening sheep to study the effects of GAS on gut microbiota, serum and urine metabolism, and transcriptome of rumen epithelial tissue in fattening sheep. Twelve Hu sheep (body weights; BW, 23.0 ± 2.3 kg and ages 120 ± 3.5 d) were randomly divided into two groups. The CON group was the basal diet, while the GAS group was supplemented with GAS in the basal diet. The trial period was 10 weeks, with the first 2 weeks being the pre-trial period.

Results

The daily average weight gain of fattening sheep in the GAS group was significantly higher than that in the CON group (p < 0.05), and the serum GSH-Px of the GAS group fattening sheep was significantly increased, while MDA was significantly reduced (p < 0.05). Based on the genus classification level, the addition of garlic peel in the diet changed the intestinal microbial composition, and the relative abundance was significantly upregulated by Metanobrevibater (p < 0.05), while significantly downregulated by Akkermansia, Parasutterella, and Guggenheimella (p < 0.05). Metabolomics analysis found that there were 166 significantly different metabolites in serum and 68 significantly different metabolites in urine between the GAS and CON groups (p < 0.05). GAS had an impact on amino acid metabolism, pyrimidine metabolism, methane metabolism, riboflavin metabolism, and unsaturated fatty acid synthesis pathways (p < 0.05). Transcriptome sequencing showed that differentially expressed genes were mainly enriched in immune regulatory function, improving the health of fattening sheep.

Conclusion

Adding GAS can improve the energy metabolism and immune function of fattening sheep by altering gut microbiota, metabolome, and transcriptome, thereby improving the growth performance of fattening sheep.
GnRH-mediated suppression of S100A4 expression inhibits endometrial epithelial cell proliferation in sheep via GNAI2/MAPK signaling
Authors: Jiao Xiyao , Chu Zhili , Li Meng , Wang Jiurong , Ren Zilong , Wang Leyang , Lu Chengcheng , Li Xiangyun , Ren Feng , Wu Xinglong
Published: May 2024

Background

Gonadotrophin-releasing hormone (GnRH) administration significantly decreases the pregnancy rate of recipient ewes after embryo transfer, possibly because GnRH affects endometrial epithelial cell function. Therefore, this study investigated the effect of GnRH on endometrial epithelial cells.

Methods

Transcriptome sequencing was used to determine the regulatory effect of GnRH on the ewe endometrium, and the S100A4 gene, which showed altered transcription, was screened as a candidate regulator of this effect. Endometrial epithelial cells were further isolated, the S100A4 protein was immunoprecipitated, and host proteins that interacted with S100A4 were identified by mass spectrometry. We further verified the effects of S100A4 and GNAI2 on the proliferation of endometrial epithelial cells via overexpression/knockdown experiments and subsequent CCK-8 and EdU assays. The effect of S100A4 deletion in endometrial cells on reproduction was verified in mice with S100A4 knockout.

Results

Our results showed that S100A4 gene transcription in endometrial cells was significantly inhibited after GnRH administration. GNAI2 was identified as a downstream interacting protein of S100A4, and S100A4 was confirmed to activate the MAPK signaling pathway to promote cell proliferation by targeting GNAI2.

Conclusion

GnRH can suppress the expression of S100A4 in the endometrium, consequently inhibiting the proliferation of endometrial cells through the S100A4/GNAI2/MAPK signaling pathway. These findings suggest a potential explanation for the limited efficacy of GnRH in promoting embryo implantation.
Can Nitrogen Fertilization and Intercropping Modify the Quality and Nutrient Yield of Barley–Field Bean Forage?
Authors: Angeletti Francesco Giovanni Salvo , Pampana Silvia , Arduini Iduna , Saia Sergio , Mariotti Marco
Published: May 2024

Barley (Hordeum vulgare L.) and field bean (Vicia faba L. var minor) are often used for forage production in the Mediterranean environment. Their bromatological and productive characteristics are known when cultivated as sole crops, but if grown simultaneously in intercropping, the changes in their morphological and physiological characteristics could affect the quality and the nutrient yield of the resulting forages. In a two-year field research in Central Italy, we determined the bromatological traits and nutrient yields of barley and field bean, grown as sole crops or intercrops in a 1:1 additive design harvested at the heading and early dough stage with five nitrogen (N) rates (i.e., from 0 to 200 kg ha−1). Both intercropping and N fertilization increased the concentration of crude protein and fiber but decreased the general quality of the forage. However, the effects on nutrient yields were more marked; those of crude protein and total digestible nutrients increased by 46 and 29% with intercropping and by 49 and 46% with 150 kg N ha−1. Thus, we concluded that N fertilization should not exceed 50 kg ha−1 to maximize the relative feed value, while 150 kg ha−1 are suitable to boost nutrient yields.
Silicon Improves Heat and Drought Stress Tolerance Associated with Antioxidant Enzyme Activity and Root Viability in Creeping Bentgrass (Agrostis stolonifera L.)
Authors: Zhang Xunzhong , Goatley Mike , Wang Kehua , Goddard Ben , Harvey Rose , Brown Isabel , Kosiarski Kelly
Published: May 2024

Creeping bentgrass (Agrostis stolonifera L.) is an important cool-season turfgrass species widely used for golf course putting greens; however, it experiences summer stress and quality decline in the U.S. transition zone and other regions with similar climates. Silicon (Si) may improve the abiotic stress of creeping bentgrass, but the mechanism of its impact on plant drought and heat tolerance is not well understood, and a few studies have reported on the effects of Si on creeping bentgrass drought and heat tolerance. The objectives of this study were to determine the effects of Ortho-silicic acid (Ortho-Si) on antioxidant metabolism and root growth characteristics and viability in creeping bentgrass under drought and heat-stress conditions. The three treatments, including control, Ortho-Si at 0.16 mL m−2 and 0.32 mL m−2, were applied biweekly to creeping bentgrass. Foliar application of the Ortho-Si exhibited beneficial effects on turf quality, physiological fitness, and root growth in creeping bentgrass. The Ortho-Si application at 0.16 mL m−2 and 0.32 mL m−2 improved turf quality ratings by 9.5% and 11.1%, respectively, photochemical efficiency (PE) by 6.9% and 8.5%, respectively, chlorophyll content by 27.1% and 29.9%, and carotenoids content by 25.5% and 27.2%, respectively, when compared to the control at the end of the trial. The Ortho-Si treatments enhanced antioxidant enzyme activity; the highest amount, in particular, increased superoxide dismutase (SOD) activity by 32.8%, catalase (CAT) by 12.8%, and ascorbate peroxidase (APX) activity by 37.4%, as compared to the control. The Ortho-Si application reduced leaf hydrogen peroxide (H2O2) concentration relative to the control. In addition, exogenous Ortho-Si improved leaf Si concentration. The Ortho-Si application at 0.32 mL m−2 increased root biomass by 52.7% and viability by 89.3% relative to the control. Overall, Ortho-Si at 0.32 mL m−2 had greater beneficial effects than the low rate (0.16 mL m−2). Exogenous Si may improve drought and heat tolerance by protecting photosynthetic function, enhancing the activities of leaf antioxidant enzymes, and stimulating root growth, viability, and Si uptake. The results of this study suggest that foliar application of Ortho-Si at 0.32 mL m−2 may be considered to be an effective approach to improve turf quality and physiological fitness of creeping bentgrass during the summer months in the U.S. transition zone and other regions with similar climates.
Lycium barbarum (Wolfberry) Branches and Leaves Enhance the Growth Performance and Improve the Rumen Microbiota in Hu Sheep
Authors: Duan Pingping , Rehemujiang Halidai , Zhang Lidong , Lu Mulong , Li Changchang , Hu Lihong , Wang Youli , Diao Qiyu , Xu Guishan
Published: May 2024

The Lycium barbarum branches and leaves (LBL) are known to contain a range of active substances that have positive effects on animal immunity and antioxidation. This study aimed to examine how LBL impacts the growth and slaughter performance as well as rumen fermentation and microbiota in Hu sheep. A total of 50 male Hu sheep of indigenous origin, aged 3 months, were randomly divided into 5 groups of 10 sheep each. The groups were given different levels of LBL supplementation (0%, 3%, 6%, 9%, and 12%) to evaluate growth performance and nutrient apparent digestibility. Rumen fluid samples were collected for analysis of the fermentation parameters and rumen chyme was examined to study the rumen microbiota. The slaughter performance, meat quality, and organ index were evaluated at the conclusion of the experiment. The results showed that the final body weight and average daily gain of the LBL1 group were significantly higher than those of the CON group, LBL3 group, and LBL4 group (p < 0.05). The average dry matter intake of the LBL4 group was significantly lower than that of other experimental groups (p < 0.05). The apparent digestibility of CP in the LBL1 and LBL2 groups was higher than that in other experimental groups (p < 0.05). At the same time, the eye muscle area and grade-rule (GR) value of Hu sheep in the LBL1 group significantly increased and the quality of Hu sheep meat improved (p < 0.05). There was no significant difference in organ weight and organ index between the experimental groups (p > 0.05). The pH of the rumen fluid in the LBL1 group was significantly lower than that in the CON group (p < 0.05). There was no significant difference in the NH3-N content between the experimental groups (p > 0.05). The propionate and valerate in the rumen fluid of Hu sheep in the LBL2 group were significantly higher than those in other experimental groups (p < 0.05). In addition, this had no significant effect on the structure and abundance of the rumen microbiota (p > 0.05). LBL is a promising functional feed. Adding an appropriate amount of LBL to the diet can improve the feed efficiency, growth performance, and meat quality of Hu sheep but has no adverse effects on the rumen. In this experiment, the appropriate supplemental level of LBL in the diet was 3%.
Spatial Dynamics of Demersal Fish Assemblages off the Korean Coast in the East Sea
Authors: Park Joo Myun , Lee Chung Il , Park Jong Won , Jung Hae Kun , Han In Seong
Published: May 2024

This study assessed variations in demersal fish assemblages with respect to the study site and water depth. Seasonal samplings from May 2018 to March 2019 were conducted along the northern (Ayajin, Goseong) and southern (Hupo, Uljin) sites of the East Sea off the Korean coast, using commercial gill nets. Samples were collected at depths of ~50, ~80, ~150 m across the study sites, with concurrent monitoring of water column structures. A total of 73 species and 6250 specimens were collected. Distinctive fish species compositions were observed according to the study site and depth. Although Glyptocephalus stelleri was the most abundant fish species in both Ayajin and Hupo, Gadus macrocephalus, Icelus cataphractus, and Alcichthys elongatus were most predominant in Ayajin, whereas Cleisthenes pinetorum, Hippoglossoides dubius, and Gymnocanthus herzensteini were more prevalent in Hupo. In terms of depth layer, in Ayajin, G. stelleri dominated in both intermediate and deeper layers, with Hemilepidotus gilberti, A. elongatus, Enophrys diceraus common in shallower depths. Conversely, in Hupo, G. stelleri, C. pinetorum, and A. nadeshnyi dominated across all depth layers, whereas Dasycottus setiger and G. herzensteini dominated in deeper and shallower depths, respectively. Significant influences of the study site and water depth on fish assemblage structures were observed due to variations in water temperature at the seasonal thermocline boundary.
Improving Efficiency: Automatic Intelligent Weighing System as a Replacement for Manual Pig Weighing
Authors: Hou Gaifeng , Li Rui , Tian Mingzhou , Ding Jing , Zhang Xingfu , Yang Bin , Chen Chunyu , Huang Ruilin , Yin Yulong
Published: May 2024

To verify the accuracy of AIWS, we weighed 106 pen growing-finishing pigs’ weights using both the manual and AIWS methods, respectively. Accuracy was evaluated based on the values of MAE, MAPE, and RMSE. In the growth experiment, manual weighing was conducted every two weeks and AIWS predicted weight data was recorded daily, followed by fitting the growth curves. The results showed that MAE, MAPE, and RMSE values for 60 to 120 kg pigs were 3.48 kg, 3.71%, and 4.43 kg, respectively. The correlation coefficient r between the AIWS and manual method was 0.9410, and R2 was 0.8854. The two were extremely significant correlations (p < 0.001). In growth curve fitting, the AIWS method has lower AIC and BIC values than the manual method. The Logistic model by AIWS was the best-fit model. The age and body weight at the inflection point of the best-fit model were 164.46 d and 93.45 kg, respectively. The maximum growth rate was 831.66 g/d. In summary, AIWS can accurately predict pigs’ body weights in actual production and has a better fitting effect on the growth curves of growing-finishing pigs. This study suggested that it was feasible for AIWS to replace manual weighing to measure the weight of 50 to 120 kg live pigs in large-scale farming.
Impact of a Local Government Funded Free Cat Sterilization Program for Owned and Semi-Owned Cats
Authors: Cotterell Jennifer L. , Rand Jacquie , Barnes Tamsin S. , Scotney Rebekah
Published: May 2024

In most states of Australia, local governments (councils) are responsible for the enforcement of legislation relating to domestic cats. Traditional methods used for cat management based on trap–adopt or euthanize programs have been ineffective, with cat-related calls and cat impoundments continuing to increase, resulting in many healthy cats being euthanized. This has detrimental effects on the mental health of animal management officers, staff in shelters and council facilities, and cat caregivers. The city of Banyule, Victoria, implemented a free cat sterilization, microchipping, and registration (licensing) program in 2013/14. Initially, it was targeted at three low-socioeconomic suburbs with the highest cat-related calls and intake, and was microtargeted at call locations. An average of 4.1 cats/1000 residents per year were sterilized over eight years. The program included stray cats being fed by caregivers, provided they took ownership. The program was later expanded city-wide. Over eight years, city-wide cat intake decreased by 66%, euthanasia by 82%, and cats reclaimed by owners increased from 6% of intake (2012/13) to 16% (2020/21). Cat-related calls decreased in the target area by 51%, and city-wide by 36%. The council realized cost savings of AU $440,660 associated with reduced costs for cat-related calls to council (AU $137,170) and charges from the contracted welfare agency (AU $303,490), for an outlay of AU $77,490 for sterilization costs. Instead of the traditional management of urban cats, proactive management based on targeted sterilization should be utilized by government and animal welfare agencies in Australia and internationally. These types of programs are effective at reducing cat intake and euthanasia and are cost-effective.
A Review of the Nutritional Aspects and Composition of the Meat, Liver and Fat of Buffaloes in the Amazon
Authors: Rodrigues Laurena Silva , Silva Jamile Andrea Rodrigues da , Silva Welligton Conceição da , Silva Éder Bruno Rebelo da , Belo Tatiane Silva , Sousa Carlos Eduardo Lima , Rodrigues Thomaz Cyro Guimarães de Carvalho , Silva André Guimarães Maciel e , Prates José António Mestre , Lourenço-Júnior José de Brito
Published: May 2024

Thus, this review aims to deepen the understanding of buffalo farming in the Amazon, presenting the quality and nutritional value of buffalo meat and liver. This information serves as a subsidy to improve practices related to the breeding system, nutrition, health and sustainability associated with aquatic buffaloes. For this, a review of the databases was carried out using the descriptors “nutritional value of buffalo meat”, “nutritional value of buffalo liver” and “buffalo breeding in the Amazon”. Thus, the consumption of foods derived from aquatic buffaloes has important nutritional value for human consumption. In view of this, it is possible to conclude that the nutrition of these animals is influenced by the biodiversity of the Amazon, giving unique characteristics to its products, also highlighting the importance of carrying out research that aims to value the potential use of this species and strengthen the economy of the region.
An Integrated Analysis of Abattoir Lung Lesion Scores and Antimicrobial Use in Italian Heavy Pig Finishing Farms
Authors: Recchia Matteo , Ghidini Sergio , Romeo Claudia , Scali Federico , Maisano Antonio Marco , Guadagno Federica , De Luca Silvio , Ianieri Adriana , Alborali Giovanni Loris
Published: May 2024

Respiratory diseases significantly affect intensive pig finishing farms, causing production losses and increased antimicrobial use (AMU). Lesion scoring at slaughter has been recognized as a beneficial practice to evaluate herd management. The integrated analysis of abattoir lesion scores and AMU data could improve decision-making by providing feedback to veterinarians and farmers on the effectiveness of antimicrobial treatments, thus rationalizing their use. This study compared lung and pleural lesion scores collected at Italian pig slaughterhouses with on-farm AMU, estimated through a treatment index per 100 days (TI100). Overall, 24,752 pig carcasses, belonging to 236 batches from 113 finishing farms, were inspected. Bronchopneumonia and chronic pleuritis were detected in 55% and 48% of the examined pigs, respectively. Antimicrobials were administered in 97% of the farms during the six months prior to slaughter (median TI100 = 5.2), notwithstanding compliance with the mandatory withdrawal period. EMA category B (critical) antimicrobials were administered in 15.2% of cases (median TI100 = 0.06). The lung score was not associated with the total AMU, but significant, positive associations were found with the past use of critical antimicrobials (p = 0.041) and macrolides (p = 0.044). This result highlights the potential of abattoir lung lesion monitoring to rationalize antimicrobial stewardship efforts, contributing to AMU reduction.
La Tène Horse Remains from Alba Iulia CX 143 Complex: A Whole Story to Tell
Authors: Gudea Alexandru Ion , Bârcă Vitalie , Irimie Alexandra , Martonos Cristian Olimpiu , Socaciu Antonia
Published: May 2024

The present paper deals with the archaeozoological investigation carried out on a horse skeleton discovered in a Late Iron Age La Tène tomb (coded CX 143) in Alba Iulia, Romania. The paper presents all the results of the investigation, with a description of finds, adding a detailed assessment of the dentition with some interesting conclusions on the usage of a horse bit and the possible consequences of this use. The morphological features of the horse indicate a 7–8-year-old male individual, with a recalculated height of 1200–1300 mm. What is also stressed in the investigated sample is the lack of the characteristic pathological lesions typical for horseback riding but showing distinctive elements of bit wear. A comparative perspective over the few findings from the same period is provided to ensure the framing of the identified individual into the much larger historical context.
Suppliers’ Perspectives on Cage-Free Eggs in China
Authors: Chen Maria , Lee Huipin , Liu Yuchen , Weary Daniel M.
Published: May 2024

Successful promotion of cage-free eggs supports a housing system offering potential for improved hen welfare. As the world’s largest egg producer and consumer, China offers much potential for welfare improvements. We examined 10 Chinese companies supplying cage-free eggs (four using indoor systems, six with outdoor access) to understand their strategies to promote cage-free eggs to businesses and consumers. We purposively sampled 12 employees from these companies familiar with production or sales. We conducted two–three semi-structured interviews per participant, collected public online documents (including online shops and social media content), and recorded field notes. We analyzed the data using template analysis to generate key results. Participants reported buyers being unfamiliar with ‘animal welfare’ and ‘cage-free’, but familiar with concepts associated with ‘free-range’. Participants considered three attributes when promoting cage-free eggs: price (engaging buyers who were willing to pay more), experiential attributes (e.g., taste, accommodating buyer preferences), and non-sensory credence attributes (e.g., cage-free production, improving buyers’ understanding and trust). Our results are not generalizable, though they may be transferable to similar contexts. Understanding how companies promoted cage-free eggs to buyers may help inform promotion of other animal products with welfare attributes. Simultaneous efforts are needed to ensure actual welfare improvements on farms.
Effect of Transcutaneous Electrical Nerve Stimulation on Gait Parameters in Dogs with Osteoarthritis
Authors: Pedersen Anja , Hyytiäinen Heli K. , Rhodin Marie , Forterre Franck , Penell Johanna , Bergh Anna
Published: May 2024

Osteoarthritis is a common degenerative disease in dogs, often manifested as pain, joint swelling, and lameness. Despite the lack of scientific evidence for its treatment efficacy, transcutaneous electrical nerve stimulation (TENS) is used in dogs as a pain-relieving treatment. This randomised single-blinded cross-over study investigated the effect of TENS on gait parameters in fifteen dogs with osteoarthritis. Stance time, swing time, stride time, stride length, peak vertical force (%BW), vertical impulse (%BW*sec), and symmetry indices were obtained using a pressure-sensitive mat. TENS treatment of 80 Hz and 100 µs with an individually selected amplitude was conducted for 45 min once daily for a treatment period of seven or ten days. No significant differences were seen between TENS and placebo for any of the gait parameters. Hence, in this study, TENS did not affect gait parameters, compared to placebo. Further studies are needed to confirm the observations.
Consumers’ Perceptions about Edible Insects’ Nutritional Value and Health Effects: Study Involving 14 Countries
Authors: Guiné Raquel P. F. , Florença Sofia G. , Costa Cristina A. , Correia Paula M. R. , Boustani Nada M. , Matran Irina , Jakšić Krešimir , Chuck-Hernández Cristina , Bartkiene Elena , Djekic Ilija , Papageorgiou Maria , Arias Leticia G. , Korzeniowska Malgorzata , Černelič-Bizjak Maša , Klava Dace , Ferreira Vanessa , Damarli Emel , Ferreira Manuela
Published: May 2024

Insects have been consumed for time immemorial in many regions of the globe. However, in other parts, they are not traditionally eaten. Because they are a more sustainable source of animal protein and provide valuable nutrients as well as bioactive compounds with beneficial effects on the human body, their consumption is encouraged. Knowledge can serve as a tool for better acceptance of insects as food. In this context, the present work investigated the knowledge about the nutritional value and health effects of edible insects in different countries. Data were collected by employing a questionnaire survey translated into the different languages of all participating countries and were treated using statistical tools. A total of 7222 responses were obtained. The results indicated that for many issues, the participants manifested a neutral opinion (neither agree nor disagree), but the participants who manifested agreement/disagreement were generally well informed. They were also able to identify untrue facts and answer accordingly by disagreeing. Factor analysis showed four groups of questions: nutritive value, negative perception and risks, safety and benefits of insects and contamination and harmful components. Finally, significant differences were observed according to the sociodemographic variables studies (sex, age, education, living environment and country), with age and country being the most influential of the sociodemographic factors on knowledge. Therefore, increasing knowledge is envisaged as an essential factor in augmenting the recognition of edible insects as a nutritional food, presenting health benefits apart from being a more sustainable source of animal protein when compared with beef or pork meats.
Possible Shifts in the Genetic Diversity of Red-crowned Cranes (Grus japonensis) in Hokkaido, Japan: Indications of Continental Gene Flow
Authors: Dong Wenjing , Tomita Kai , Sawada Akira , Hasebe Makoto , Inoue Masako , Momose Kunikazu , Nakamura Tatsuro , Teraoka Hiroki
Published: May 2024

Red-crowned cranes (Grus japonensis) consist of two distinct groups: the continental population and the island population. The island population, localized in Hokkaido, Japan, exhibits very low genetic diversity due to its rapid recovery from the brink of extinction. Our previous research in 2018 highlighted a possible mating between a male from the continental population, with the Gj5 haplotype, and a female from the island population, with the Gj2 haplotype, at Hitominuma Sawmp shore in northern Hokkaido. The present study attempted to unravel the distribution of their offspring by examining the major histocompatibility complex (MHC) of this mixed breeding pair compared with samples collected from cranes in northern and southeastern Hokkaido between 2008 and 2022. The analysis identified 55 MHC types, including 10 known types in a dataset of 89 crane samples, based on amino acid sequences. A total of 58 MHC types were recognized, based on nucleotide sequences, as there were many cases in which the same amino acid sequence had different nucleotide sequences. The five DNA types of MHC in the Hitominuma Swamp male were predominantly identified in eight cranes from northern Hokkaido and one chick from southeastern Hokkaido. In addition, population genetic analysis, based on insertion/deletion (InDel) polymorphisms, indicates distinct population differentiation between the northern and southeastern regions of Hokkaido. These results suggest that genetic contributions from the continental red-crowned crane population have already been integrated into the Hokkaido populations, with a more pronounced influence in northern Hokkaido.
Prevalence and Molecular Characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Cattle in Heilongjiang Province, Northeast China
Authors: Gao Jun-Feng , Zhou Lu , Zhang Ai-Hui , Hou Mei-Ru , Liu Xue-Wei , Zhang Xin-Hui , Wang Jia-Wen , Wang Xue , Bai Xue , Jiao Chen-Long , Yang Yan , Lan Zhuo , Qiu Hong-Yu , Wang Chun-Ren
Published: May 2024

Crytosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi are important diarrheal pathogens with a global distribution that threatens the health of humans and animals. Despite cattle being potential transmission hosts of these protozoans, the associated risks to public health have been neglected. In the present study, a total of 1155 cattle fecal samples were collected from 13 administrative regions of Heilongjiang Province. The prevalence of Cryptosporidium spp., G. duodenalis, and E. bieneusi were 5.5% (64/1155; 95% CI: 4.2–6.9), 3.8% (44/1155; 95% CI: 2.7–4.9), and 6.5% (75/1155; 95% CI: 5.1–7.9), respectively. Among these positive fecal samples, five Cryptosporidium species (C. andersoni, C. bovis, C. ryanae, C. parvum, and C. occultus), two G. duodenalis assemblages (E and A), and eight E. bieneusi genotypes (BEB4, BEB6, BEB8, J, I, CHS7, CHS8, and COS-I) were identified. Phylogenetic analysis showed that all eight genotypes of E. bieneusi identified in the present study belonged to group 2. It is worth noting that some species/genotypes of these intestinal protozoans are zoonotic, suggesting a risk of zoonotic disease transmission in endemic areas. The findings expanded our understanding of the genetic composition and zoonotic potential of Cryptosporidium spp., G. duodenalis, and E. bieneusi in cattle in Heilongjiang Province.
Enhancing the Anti-Tumor Efficacy of NK Cells on Canine Mammary Tumors through Resveratrol Activation
Authors: Zhu Tingting , Jin Shengzi , Tong Danning , Liu Xingyao , Liu Yun , Zheng Jiasan
Published: May 2024

In order to explore the therapeutic effect of Resveratrol (Res)-activated Natural Killer (NK) cells on canine mammary tumors, this study employed a range of assays, including wound healing, colony formation, Transwell, flow cytometry, and Western blot experiments, to investigate the impact of Res-pretreated NK cells on canine mammary tumor cells in vitro. Additionally, a tumor-bearing mouse model was utilized to further analyze the therapeutic effects of Res-pretreated NK cells in vivo. The results showed that Res enhances the capacity of NK cells to induce apoptosis, pyroptosis, and ferroptosis in canine breast tumor cells, while also augmenting their influence on the migration, invasion, and epithelial-mesenchymal transition of these cells. Furthermore, pretreatment of NK cells with Res significantly amplified their inhibitory effect on breast tumor growth in vivo and promoted tumor tissue apoptosis. Additionally, Res enhanced the recruitment of NK cells to other immune cells in the body. In summary, Res has been shown to enhance the anti-breast-tumor effect of NK cells both in vitro and in vivo, offering a new avenue for optimizing immunotherapy for canine breast tumors.
Five-Year Evaluation of the PROA-NEN Pediatric Antimicrobial Stewardship Program in a Spanish Tertiary Hospital
Authors: Fernández-Polo Aurora , Melendo-Perez Susana , Larrosa Escartin Nieves , Mendoza-Palomar Natalia , Frick Marie Antoinette , Soler-Palacin Pere ,   on behalf of the PROA-NEN Working Group
Published: May 2024

Introduction: Actions to reduce and optimize antimicrobial use are crucial in the management of infectious diseases to counteract the emergence of short- and long-term resistance. This is particularly important for pediatric patients due to the increasing incidence of serious infections caused by resistant bacteria in this population. The aim of this study was to evaluate the impact of a pediatric antimicrobial stewardship program (PROA-NEN) implemented in a Spanish tertiary hospital by assessing the use of systemic antimicrobials, clinical indicators, antimicrobial resistance, and costs. Methods: In this quasi-experimental, single-center study, we included pediatric patients (0–18 years) admitted to specialized pediatric medical and surgical units, as well as pediatric and neonatal intensive care units, from January 2015 to December 2019. The impact of the PROA-NEN program was assessed using process (consumption trends and prescription quality) and outcome indicators (clinical and microbiological). Antibiotic prescription quality was determined using quarterly point prevalence cross-sectional analyses. Results: Total antimicrobial consumption decreased during the initial three years of the PROA-NEN program, followed by a slight rebound in 2019. This decrease was particularly evident in intensive care and surgical units. Antibiotic use, according to the WHO Access, Watch and Reserve (AWaRe) classification, remained stable during the study period. The overall rate of appropriate prescription was 83.2%, with a significant increase over the study period. Clinical indicators did not substantially change over the study period. Direct antimicrobial expenses decreased by 27.3% from 2015 to 2019. Conclusions: The PROA-NEN program was associated with reduced antimicrobial consumption, improved appropriate use, and decreased costs without compromising clinical and/or microbiological outcomes in patients.
Coordinating Obstacle Avoidance of a Redundant Dual-Arm Nursing-Care Robot
Authors: Yang Zhiqiang , Lu Hao , Wang Pengpeng , Guo Shijie
Published: May 2024

Collision safety is an essential issue for dual-arm nursing-care robots. However, for coordinating operations, there is no suitable method to synchronously avoid collisions between two arms (self-collision) and collisions between an arm and the environment (environment-collision). Therefore, based on the self-motion characteristics of the dual-arm robot’s redundant arms, an improved motion controlling algorithm is proposed. This study introduces several key improvements to existing methods. Firstly, the volume of the robotic arms was modeled using a capsule-enveloping method to more accurately reflect their actual structure. Secondly, the gradient projection method was applied in the kinematic analysis to calculate the shortest distances between the left arm, right arm, and the environment, ensuring effective avoidance of the self-collision and environment-collision. Additionally, distance thresholds were introduced to evaluate collision risks, and a velocity weight was used to control the smooth coordinating arm motion. After that, experiments of coordinating obstacle avoidance showed that when the redundant dual-arm robot is holding an object, the coordinating operation was completed while avoiding self-collision and environment-collision. The collision-avoidance method could provide potential benefits for various scenarios, such as medical robots and rehabilitating robots.