bluuebunny/crossref_metadata_2025 huggingface dataset:
A Aktivisme Masyarakat Desa Wadas Dalam Menolak Pertambangan Sebagai Bagian Dari Ruang Politik
Authors: Mandela Muhamad Fathan , . Ridwan
Published: December 2023

Abstrak Artikel ini menulis tentang konflik agraria yang terjadi di Desa Wadas, konflik bermula dari rencana pemerintah untuk membangun bendungan bener di Kabupaten Purworejo, pembangunan bendungan tersebut memerlukan material batuan andesit yang oleh pemerintah Desa Wadas dijadikan tempat untuk pertambangan batuan tersebut. Masyarakat Desa Wadas menilai pertambangan tersebut hanyak akan merusak lingkungan dan menghilangkan mata penceharian mereka. Konflik tersebut memuncak pada tahun lalu dengan penangkapan puluhan masyarakat akibat menolak pertambangan, artikel ini mempunyai tujuan untuk melihat aktivisme masyarakat Desa Wadas dalam memperjuangkan tanah mereka dengan pendekatan teori Ruang Politik, metode yang digunakan adalah kualitatif dengan pendekatan deskriptif. Hasil dari artikel ini menunjukan bahwa masyarakat Desa Wadas memakai Ruang Politik mereka dengan bergerak bersama dalam organisasi GEMPADEWA dan Wadon Wadas, menciptakan Ruang Politik yang independen untuk melakukan berbagai aksi yang berhadapan langsung dengan pemerintah. Kesimpulan dari artikel ini menunjukan masyarakat Desa Wadas menggunakan Ruang Politik sebagai wadah perjuangan karena adanya ketidakadilan dan diskriminatif yang mereka dapatkan, sebagai bahan rekomendasi seharunya pemerintah mencari wilayah baru yang terdapat batuan andesit, karena sesuai rencana tata ruang kota menjelaskan Desa Wadas adalah Desa yang rawan bencana.
Aproximações sobre formação de mulheres professoras no Instituto de Educação em Belém (PA) e na Escola Franciscana Imaculada Conceição de Dourados (MS) (1974 a 1991)
Authors: Pizatto Adriana Mendonca , Furtado Alessandra Cristina , Corrêa Rosa Lydia Teixeira
Published: December 2023

Este artigo analisa a formação de professores nos cursos de magistério do Instituto de Educação em Belém, no estado de Pará e da Escola Franciscana Imaculada Conceição de Dourados, no estado de Mato Grosso do Sul, com o intuito de compreender a participação das mulheres nesses dois cursos, no período de 1974 a 1991. Tem como cerne das questões norteadoras se houve ou não a presença de mulheres e consequente feminização para o magistério nesses cursos. Metodologicamente, o trabalho foi realizado por meio das pesquisas bibliográfica e documental. Os resultados indicaram que a presença de mulheres nos dois cursos se deu de modo significativo, o que permite concluir, que, embora em números diferenciados, a formação nas duas instituições acompanha, e, ao mesmo tempo alarga uma tendência em curso desde as primeiras décadas do século XX.
Support for autonomy and fear of failure in Physical Education lessons. Differences between “gender and physical extracurricular sports [Apoyo a la autonomía y miedo al fallo en las clases de Educación Física: diferencias en función del género y la práctica deportiva extraescolar]

Authors: Manzano-Sánchez David , Ferrer-López Bienvenida , Battaglia Giuseppe , Gómez-López Manuel
Published: December 2023

El propósito del estudio fue analizar la relación entre el apoyo a la autonomía percibido por estudiantes de Educación Física de Secundaria y el nivel de miedo al fallo que experimentan así como averiguar las diferencias existentes en función del sexo y la práctica deportiva extraescolar del estudiante. Para ello se administró el Cuestionario de Apoyo a la Autonomía y el Inventario de Evaluación del Error en el Rendimiento. La muestra estuvo formada por 607 estudiantes de secundaria con edades comprendidas entre 12 y 18 años (M = 14.911), siendo 286 chicos y 321 chicas. Los resultados reflejaron que las chicas tuvieron un mayor miedo a experimentar vergüenza que los chicos, los cuales mostraron un mayor miedo a perturbar a otros importantes que ellas. A su vez, el análisis de regresión lineal reflejó que el apoyo a la autonomía fue predictor del miedo al fallo excepto para experimentar vergüenza en las personas que no practican deporte extraescolar, no viéndose relación entre los que realizan práctica deportiva. Se concluye que un clima de apoyo a la autonomía contribuye a reducir el miedo al fallo de los estudiantes, especialmente entre los que no realizan actividad deportiva extraescolar.
Abstract
The purpose of the study was to analyze the relationship between the support for autonomy perceived by Secondary Physical Education students and the level of fear of failure they experience, as well as to find out the differences that exist depending on the student's sex and extracurricular sports practice. For this purpose, the Autonomy Support Questionnaire and the Performance Error Evaluation Inventory were administered. The sample consisted of 607 high school students between the ages of 12 and 18 (M = 14.91; DT = 1.46), with 286 boys and 321 girls. The results reflected that girls had a greater fear of experiencing shame than boys, who showed a greater fear of disturbing others important to them. In turn, the linear regression analysis reflected that support for autonomy was a predictor of fear of failure except for experiencing shame in people who do not practice extracurricular sports, with no relationship seen among those who practice sports. It is concluded that a climate of support for autonomy contributes to reducing students' fear of failure, especially among those who do not carry out extracurricular sports activities.
"Brain Fog" na síndrome pós COVID-19: uma revisão bibliográfica
Authors: Alves Junior Valdir Donizeti , Campos Ana Sofia Arradi , Freire Ananda Soares de Brito , Matos Isabella La-Côrte Morais , Silva Kescya Miranda de Paula
Published: December 2023

Introdução: Estudos indicam alta incidência de sintomas psiquiátricos agudos pós-infecção por SARS-CoV-2. Aqueles que apresentam sintomas persistentes e/ou disfunção orgânica após a fase aguda da COVID-19 são diagnosticados com a síndrome pós-Covid. O comprometimento cognitivo nessa síndrome inclui déficit de memória recente, dificuldades de concentração e execução, além de raciocínio lento em comparação com o padrão anterior de funcionamento. Objetivo: Fornecer uma visão geral sobre o tema “Brain Fog” e, dessa forma, contribuir para o conhecimento dos profissionais de saúde e para o desenvolvimento de estratégias de manejo em saúde mental. Método: Trata-se de uma revisão narrativa da literatura, com coleta de dados feita nas bases de dados PubMed, LILACS e Scielo. Foram selecionados 14 trabalhos, os quais contemplavam os seguintes itens sobre o tema: epidemiologia, clínica, prognóstico e tratamento. Resultado: Observou-se que o sexo feminino estava associado a uma maior chance de apresentar comprometimento cognitivo pós-covid. Estratégias de tratamento incluem avaliação neuropsicológica, reabilitação cognitiva, apoio à saúde mental e sono adequado. Abordagem multidisciplinar é crucial para lidar com sintomas persistentes e retornar à vida normal. Conclusão: Observou-se alta prevalência de quadros de disfunção cognitiva no período pós-agudo da infecção por COVID-19. Houve, também, grande heterogeneidade na caracterização dos sintomas, o que torna necessário estabelecer definições consensuais acerca da condição estudada, para melhor investigação de estratégias de prevenção e tratamento.
Görtler Instability and Transition in Compressible Flows
Authors: Xu Dongdong , Ricco Pierre , Duan Lian
Published: December 2023

We present a discussion on theoretical, experimental, and computational research studies on Görtler instability and the related transition to turbulence occurring in compressible boundary layers over concave surfaces. We first examine the theoretical results on primary and secondary instabilities, emphasizing the role of receptivity, the mechanism by which external agents, such as freestream fluctuations or wall roughness, act on a boundary layer to trigger Görtler vortices. We review experimental findings obtained from measurements in supersonic and hypersonic wind tunnels and discuss studies employing numerical methods, focusing on the direct numerical simulation approach. The research in these two last sections is surveyed according to the geometrical configuration, from simple concave walls to more complex surfaces of hypersonic vehicles. The experimental investigations have been successful in the visualizations of Görtler vortices, in the measurement of the wall-heat transfer in the transitional region, and in the computation of the Görtler-vortex growth rates, although detailed boundary-layer velocity measurements are still missing. Direct numerical simulations have confirmed instability results emerging from stability theories and revealed nonlinear interactions between Görtler vortices and other disturbances. The established initial-boundary-value receptivity theory can certainly benefit from more advanced experimental measurements, and receptivity results should be used in combination with direct numerical simulations. A major conclusion of our review is therefore that the understanding of Görtler vortices should be pursued by a combined methodology including theoretical analysis based on the receptivity formalism, direct numerical simulation, and experiments. Highly desirable outcomes of such endeavor are the prediction of the location and extension of the transition region, and a model for the transition process. We finally highlight further prospects and challenges on fundamental and applied research on Görtler instability and transition in compressible flows.
Experimental Investigation of Control Effects of Flapping Jets on Supersonic Cavity Flow
Authors: Oka Yoshinori , Ozawa Yuta , Handa Taro , Nonomura Taku
Published: December 2023

The present study investigated the effects of active flow control by high-frequency flapping jets on a supersonic cavity flow. The discharging position was varied at the leading edge, the bottom wall upstream side, the bottom-wall downstream side, and the trailing edge. The flapping jets’ frequencies were varied at 0 (non-flapping case), 15, and 22 kHz, respectively. Discharging non-flapping jets at the leading edge and the bottom wall upstream side remarkably enhanced pressure fluctuations and density gradients. The reverse flow near the bottom wall and the amplitude of the recirculation inside the cavity were enhanced when non-flapping jets were discharged at the leading edge. Discharging non-flapping jets at the bottom wall downstream side reduced the pressure fluctuations of the frequency lower than 8 kHz. Discharging flapping jets changed flowfields, although the flow control effects of discharging positions were dominant. The flapping-jet discharging at the leading edge reduced the size of the primary recirculation. Discharging flapping jets at the bottom wall downstream side showed further suppression effects on fluctuations than the non-flapping-jet discharging case. There were no significant effects on the pressure fluctuations when the jets were discharged at the trailing edge.
Noise Prediction of a Distributed Propulsion System Using the Actuator Line Method
Authors: Wickersheim Robin , Keßler Manuel , Krämer Ewald
Published: December 2023

Within this study, the capability of the actuator line method to simulate the noise of a high-lift distributed propulsion system is investigated. Therefore, the blades of three tractor propellers are approximated as lines with several nodes. Using flowfield data of computational fluid dynamic simulations, the acoustic extrapolation is carried out with the Ffowcs Williams-Hawkings approach. The obtained results are then compared to fully resolved simulations, where it is first ensured that the aerodynamic characteristics agree sufficiently. It was found that the noise characteristic is well predicted by the actuator line method, whereas discrepancies occurred in the prediction of propeller–wing interaction noise. Finally, the parameter space was extended by increasing the angle of attack for the configuration in high-lift and stall conditions. Here, the deviations in the acoustic behavior between the actuator line method and fully resolved simulations increase. Nevertheless, the qualitative characteristic of directivity is also well predicted in these conditions. Beside the main advantage of the actuator line method to perform larger propeller parameter studies without creating a body mesh, computational time can be reduced. It was found that for this configuration, a time saving of 10% could be achieved.
Experimental Investigations of Failure Behavior for Aerogel-Cored Stitched Sandwich Composites Under Vibration Conditions
Authors: Tian Zhiqiang , Zhang Peiwei , Fei Qingguo
Published: December 2023

Aerogel-cored stitched sandwich composites (ACSSCs), which possess numerous desirable properties, are anticipated to become the preferred choice for next-generation integrated thermal protection systems (ITPSs) in high-speed vehicles. Despite the reinforcement provided by stitching, the ACSSC structures on vehicles’ outer surfaces remain vulnerable to damage resulting from the excessively high deformation and vibration that occur during high-speed flight. Accordingly, experimental tests were conducted to investigate the failure characteristics of ACSSCs under bending vibration conditions. The tests demonstrated that the ACSSC structures were prone to fatigue failure caused by vibration, which resulted in significant shear damage in the aerogel core. Furthermore, the study introduced a new method for indirectly characterizing the fatigue life of shear damage in the core. Finite element analysis was undertaken to elucidate the cause of the cracking near the bottom layer of the core. The results indicated that the crack location of the aerogel core was littered with the high stiffness and deformation of internal stitches.
Interacting Linear Modes in the Turbulent Flow of an Industrial Swirled Combustor
Authors: Moczarski Lukas , Treleaven Nicholas C. W. , Oberleithner Kilian , Schmidt Simon , Fischer André , Ludwig Kaiser Thomas
Published: December 2023

Swirled flows often feature global modes that become manifest as skew-symmetric helical vortices, also known as precessing vortex cores, which dominate the flow dynamics. This study uses linear stability analysis (LSA) and bispectral mode decomposition (BMD) to elucidate the interactions of such modes in the turbulent, nonreacting, swirled flow of an industrial, three-passage, aeroengine fuel injector obtained via large-eddy simulation. A discrete Fourier transform in time retrieves the modes as narrow-banded, evenly spaced peaks in the spectral amplitude of the turbulent signal, where the second mode is dominant. Similar arrangements are known to appear as a consequence of a saturated global mode, which amplifies its higher harmonic frequencies due to nonlinear effects. We show that each mode appears in the spectrum of LSA eigenvalues, indicating that the subdominant peaks are caused not only due to nonlinear interactions but that they have an underlying linear mechanism. A structural sensitivity analysis based on the adjoint LSA shows that the observed helical modes originate close to the exit plane of the fuel injector. Finally, the BMD reveals significant nonlinear interaction between the individual modes. It is hypothesized that this interaction amplifies the modes, which are linearly stable, leading to the strong dynamics in the flow.
Funkcja cytatu z Ps 78,2 w Ewangelii Mateusza
Authors: Herok Piotr
Published: December 2023

niniejszym artykule autor bada funkcję, jaką pełni cytat z Ps 78,2 w Ewangelii Mateusza. Po zarysowaniu oryginalnego hebrajskiego kontekstu analizowanego wersetu autor porównuje go z jego starożytnymi tłumaczeniami greckimi. W kolejnym kroku przedstawia formę tekstualną i znaczenie Ps 78,2 w nowym kontekście Mt 13,35. Następnie prezentuje znaczenie badanego wersetu dla całej pierwszej Ewangelii, ze szczególnym uwzględnieniem użycia przez Mateusza terminu παραβολή oraz jego koncepcji teologicznej,dotyczącej tożsamości Jezusa, bazującej na pięciu oznaczonych cytatach z Psalmów.
Dicionário online do Kheuól do Uaçá, variedades Karipuna e Galibi-Marworno
Authors: Romling Glauber , Santos Gélsama Mara Ferreira dos , Carvalho Amanda da Costa , Forte Janina dos Santos , Silva Jaciara Santos da
Published: December 2023

O Kheuól do Uaçá é uma língua indígena ameaçada falada e compartilhada por dois povos de origens bastante distintas, os Karipuna e Galibi-Marworno, que habitam Terra Indígena do Uaçá, situada no município de Oiapoque, Estado do Amapá, na fronteira franco-brasileira. O Kheuól do Uaçá deriva do guianense, língua que surgiu em Caiena, atual Guiana Francesa, no contexto colonial dos séculos XVII-XVIII, a partir do francês (Carvalho, 2020; Santos, M.; Silva, G. 2020; Lüpke et al, 2020, Silva, G. 2021). Consolidou-se como língua franca de uma região com fronteira disputada por quase duzentos anos entre Portugal (posteriormente Brasil) e França, de 1713 até 1900. No século XX, com a consolidação da fronteira norte brasileira, firmou-se como língua de identidade desses dois povos (Silva, G. 2021). O objetivo deste trabalho é apresentar os resultados parciais do processo de construção do Dicionário Online do Kheuól do Uaçá (DOKH), um dos subprojetos do Projeto de Documentação de Línguas do Museu do Índio (FUNAI/UNESCO). A produção do DOKH, nas variedades Karipuna e Galibi-Marworno, insere-se no contexto de revitalização linguística operado nos últimos anos por professores indígenas dos dois povos, que inclui a formação de pesquisadores indígenas em nível de graduação e pós-graduação (mestrado) em métodos e técnicas de Documentação Linguística voltados para a produção de materiais didáticos e paradidáticos em Kheuól (Gippert et al., 2006; Moore et al., 2016: Santos, M.; Silva, G. 2020; Forte, 2021; Silva, J., 2022; ). A confecção do DOKH, aplica como metodologia uma abordagem participativa e formativa voltada pata a autonomia (Denzon et al. 2008; Stenzel, 2014; Franchetto, 2017; Smith, 2021). O trabalho buscará refletir sobre os impactos desse processo na revitalização, documentação e descrição dessa língua.
WHAT CAN FIGHTING TELL US ABOUT JOURNALISTIC IDENTITIES?
Authors: Pereira Fábio Henrique
Published: December 2023

ABSTRACT – In this essay we argue that journalists’ engagements, their fights, reveal, indeed, the dynamics of how their professional identities are constructed. By engaging in a struggle, the journalist seeks to state something about himself, for himself (and his profession group) and for the others (actors that participate in this public combat). The journalism fights reveal the way the professional group (or part of it) tries to manage its relationship with rival actors at the same time it expresses a public discourse about its practice, its identity. The journalism fights reveal the temporary or permanent alliance journalists establish within the professional group, as so with external actors who share the same interests. Fighting is also participating in the construction of a social problem, by converting some issues/situations specific to the professional group to a subject that interest the whole society.

RESUMO – Neste ensaio, argumentamos que o engajamento dos jornalistas, suas lutas, revelam as dinâmicas de construção das identidades profissionais. O jornalista, quando se engaja em um combate, tem a intenção de dizer algo sobre si, para si (para o grupo profissional) e para os outros (atores que participam do combate público). Os combates do jornalismo revelam a forma como o grupo profissional (ou parte dele) busca gerir as relações com atores concorrentes ao mesmo tempo em que exprime um discurso público sobre sua prática, sua identidade. Os combates do jornalismo também revelam as alianças, temporárias ou permanentes, que os jornalistas estabelecem no interior do grupo, mas também com atores externos, que partilham dos mesmos interesses. Combater é também participar da construção de um problema público, por meio da conversão de certas questões/situações específicas ao grupo profissionais em um tema de interesse da sociedade.RESUMEN – En este ensayo, sostenemos que el compromiso de los periodistas, sus luchas, revelan la dinámica de construcción de sus identidades profesionales. Cuando el periodista entra en combate, él pretende decir algo sobre sí mismo, para sí mismo (para el grupo profesional) y para los demás (actores que participan en el combate público). Las luchas del periodismo revelan la forma en que el grupo profesional (o parte de él) procura gestionar las relaciones con sus competidores mientras expresa un discurso público sobre su práctica y identidad. Los combates del periodismo también revelan las alianzas, temporales o permanentes, que los periodistas establecen dentro del grupo, pero también con actores externos, que comparten los mismos intereses. Combatir es también participar en la construcción de un problema público, a través de la conversión de determinadas cuestiones/situaciones propias del colectivo profesional en un tema de interés para la sociedad.
THE CONTRIBUTION OF LEPTIN AND INSULIN TO THE REGULATION OF THE DENSITY OF SYNAPTIC AMPA RECEPTORS IN THE HIPPOCAMPUS
Authors:   Federal Research Center for Information and Computational Technologies, Novosibirsk, Vechkapova S.O. , Proskura A.L. ,   Novosibirsk State Medical University
Published: December 2023

In these theses we consider the molecular mechanisms of the insulin and leptin receptor signaling pathways involved in the regulation of the density of synaptic AMPA receptors in the CA1 field of the hippocampus. The proposed reconstruction of molecular events makes it possible to evaluate the contributions of insulin and leptin to synaptic plasticity and maintaining the balance of excitation and inhibition in the neural networks of the hippocampus during the implementation of higher cognitive functions.
CELL-PENETRATING PEPTIDE AND CATIONIC LIPOSOMES MEDIATED SIRNA DELIVERY TO ARREST GROWTH OF CHRONIC MYELOID LEUKEMIA CELLS IN VITRO
Authors:   Smorodintsev Research Institute of Influenza, Saint Petersburg, Vysochinskaya V.V. , Zabrodskaya Y.A. , Dovbysh O.V. , Egorova M.A. , Maslov M.A. ,   Peter the Great Saint Petersburg Polytechnic University,   MIREA — Russian Technological University, Moscow
Published: December 2023

Comparison of the cell-penetrating peptide EB1 and original 2X3-DOPE-PEG cationic liposomes for anti-BCR-ABL siRNA delivery into cells of chronic myeloid leukemia showed that 2X3-DOPE-PEG cationic liposomes deliver siRNA more efficiently into K562 cells, that leads to more effective inhibition of expression of the targeted gene and cancer cell proliferation.
A COMPARATIVE STUDY OF THE IMPACT OF MICROGRAVITY AND MAGNETIC LEVITATION ON ESCHERICHIA COLI PROTEOME
Authors:   Gamaleya National Research Centre for Epidemiology and Microbiology, Moscow, Domnin P.A. , Kononikhin A.S. , Parfenov V.A. , Ermolaeva S.A. , Khesuani Yu.D. ,   Emanuel Institute of Biochemical Physics RAS, Moscow,   3D Bioprinting Solutions, Laboratory for Biotechnological Research, Moscow
Published: December 2023

Here we investigated whether it is possible to use magnetic levitation as a model of microgravity. The proteome of E. coli grown under spaceflight or combined spaceflight and magnetic force conditions were compared with ground cultivated bacteria grown under standard (control) or magnetic levitation conditions.
ASSESSMENT OF DISEASE PROGRESSION IN ESTROGEN-POSITIVE BREAST CANCER PATIENTS TREATED TAMOXIFEN
Authors:   Cancer Research Institute, Tomsk National Research Medical Center, Dronova T.A. , Babyshkina N.N. , Slonimskaya E.M. , Cherdyntseva N.V. ,   Saint Petersburg State University
Published: December 2023

The aim: to study the expression status of ERα, TGF-βR2, pAkt to assess the breast cancer progression when prescribing drug therapy with tamoxifen. ESR1 gene expression level, TGF-βR2 and pAkt protein expression as well as the populations of pAkt–/TGFβR2+ cells were identified as additional molecular genetic markers for the prognosis of estrogen-positive breast cancer.
ESTABLISHMENT AND CHARACTERIZATION OF PRIMARY UVEAL MELANOMA CELL CULTURE
Authors:   Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Zhilnikova M.V. , Troitskaya O.S. , Novak D.D. , Nushtaeva A.A. , Koval O.A. ,   Novosibirsk State University
Published: December 2023

The primary human uveal melanoma (UM) cell culture was established and key surface and intracellular markers of oncotransformed melanocytes were characterized. Based on the primary cell culture, genetically modified cell culture of UM expressing fluorescent protein mKate2 and luciferase Luc2 was created.
USING OF SILVER IONS TO STUDY COPPER METABOLISM IN MAMMALS
Authors:   ITMO University, Saint Petersburg, Ilyechova E.Yu. , Magazenkova D.N. , Rekina V.E. , Jassem Z.M. , Farroukh M. , Puchkova L.V. ,   Institute of Experimental Medicine, Saint Petersburg,   Peter the Great Saint Petersburg Polytechnic University
Published: December 2023

Copper is an essential trace element playing important roles in various biological processes. The study demonstrates the possibility of studying the copper metabolism (CM) in mammals using silver ions. The existence of inter-organ cross-talk of CM, the ability of Cu(I) and Cu(II) to be transported into the cell using different transporters, as well as the effect of copper status on tumor growth are discussed
CHROMOSOME 12 FRAGILITY AT THE ANKS1B LARGE NEURONAL GENE AND DELAYED MITOTIC ENTRY CAUSED BY REPLICATION STRESS IN HUMAN INDUCED PLURIPOTENT STEM CELLS
Authors:   Laboratory of Mutagenesis, Research Centre for Medical Genetics, Moscow, Kislova A.V. , Zheglo D. , Pozhitnova V.O. , Sviridov P.S. , Gadzhieva E.P. , Voronina E.S.
Published: December 2023

Replication stress is a feature of embryonic and induced pluripotent stem cells (iPSCs) and predispose to chromosomal instability. We characterized the mitotic entry dynamics of asynchronous iPSCs under the replication stress and mapped the FRA12L fragile site in the large neuronal ANKS1B gene. Our study provides the first evidence of common fragile site induction in iPSCs and reveals potential somatic instability of a clinically relevant gene.
CO-TRANSLATIONAL FOLDING OF MEMBRANE PROTEINS DURING CELL FREE SYNTHESIS
Authors:   Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Kornilov F.D. , Ananiev E.A. , Vasilieva E.V. , Mineev K.S. , Goncharuk S.A. , Goncharuk M.V. ,   Lomonosov Moscow State University
Published: December 2023

The biochemical obtaining of natively folded membrane proteins (MPs) is an extremely important task of modern structural biology. We have studied the efficiency of co-translational incorporation of MPs into various membrane-mimicking media — liposomes, micelles, and bicelles during the cell-free synthesis. The retinal protein of Exiguobacterium sibiricum (ESR) was chosen as an object of study.
GENERATION OF GENETICALLY MODIFIED DROSOPHILA MELANOGASTER FOR THE STUDYING OF TOOTHRIN GENE FUNCTIONS
Authors:   Koltzov Institute of Developmental Biology RAS, Moscow, Kuvaeva E.E. , Mertsalov I.B. , Simonova O.B.
Published: December 2023

toothrin (tth) belongs to the evolutionarily conserved d4 gene family. In contrast to other D4 proteins, TTH contains the only one of three family-specific conserved domains, the 2/3 domain. Since the functions of tth are unknown, we generated both tth-knockout and tth-Gal4 transgenic flies to study its null-mutants and expression pattern. Our results indicate the involvement of tth in the development and functioning of the nervous, visual and endocrine systems.
METATRANSCRIPTOMIC ANALYSIS OF THE BACTERIAL COMPOSITION OF DIFFERENT PARTS OF THE STOMACH IN PATIENTS WITH GI TRACT DISEASES
Authors:   Institute of Fundamental Medicine and Biology, Kazan Federal University, Kupriyanova E.A. , Abdulkhakov S.R. , Markelova M.I. , Grigoryeva T.V.
Published: December 2023

Gastric microbiota is supposed to play an important role in progression of inflammation and pathogenesis of upper GI disorders. However, to this date the issue remains poorly understood. The aim of this study was to characterize the microbiota of the gastric mucosa in H.pylori-positive and H.pylori-negative patients in biopsy samples from the antrum and corpus of the stomach.
THE IDENTIFICATION BY EXOME SEQUENCING OF CANDIDATE BREAST CANCER GENES IN BRCA-NEGATIVE TUVAN PATIENTS
Authors:   Cancer Research Institute of Tomsk NRMC, Molokov A.Yu. , Gervas P.A. , Cherdyntseva N.V. ,   Tomsk State University
Published: December 2023

Breast cancer (BC) occupies a leading position in terms of morbidity worldwide. At the moment, there is an active study of genes involved in the pathogenesis of breast cancer, specific for the ethnic groups of the Russian Federation. In order to search for new genes associated with hereditary breast cancer, we performed exome sequencing of the material of patients of the Tuvan ethnic group diagnosed with breast cancer. Performed exome sequencing showed that the LGR4 gene could be involved in the pathogenesis of breast cancer.
EVALUATION OF THE EFFECTIVENESS OF USING TWEEN-20 TO OBTAIN DNA-APTAMERS USING CELL SELEX TO RECEPTOR TYROSINE KINASE C-KIT
Authors:   Engelhardt Institute of Molecular Biology RAS, Moscow, Polyakov S.A. , Kuznetsova V.E. , Lebedev T.D. , Shershov V.E. , Chudinov A.V.
Published: December 2023

The degree of influence of Tween-20 on the viability of modified mouse blood cells with overexpression of human receptor tyrosine kinase c‑KIT was determined.
DEVELOPMENT OF A TEST SYSTEM FOR SCREENING BACTERIA PRODUCING BIOLOGICALLY ACTIVE NONRIBOSOMAL PEPTIDES AND POLYKETIDES
Authors:   Institute of Life Sciences and Biomedicine, Far Eastern Federal University, Vladivostok, Poteshkina K.I. , Stenkova A.M.
Published: December 2023

An important direction in science and medicine is the study of biologically active compounds produced by marine microorganisms, due to which there is a search for new promising drugs. This study is aimed at screening a collection of marine microorganisms obtained from invertebrates from the Vostok Bay of Primorsky Krai for the presence of biochemical gene clusters of nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS).
Hábitos alimentares e comportamentos sedentários entre adolescentes e suas famílias no contexto da pandemia da COVID-19
Authors: Gomes Betânia da Mata Ribeiro , Sousa Tarcísia Domingos de Araújo , Delgado Flávia Alves , Santana Luana Maria Almeida de , Vasconcelos Eliane Maria Ribeiro de , Avelar Ana Emília Alcântara de
Published: December 2023

Objetivo: Analisar e interpretar o cotidiano de famílias com filhos adolescentes, residentes em Recife, Pernambuco, durante o período de distanciamento físico, estipulado pelo Governo do Estado de Pernambuco. Métodos: Pesquisa qualitativa, realizada entre fevereiro e outubro de 2020, através de videochamadas ou telefonemas, com 23 membros de dez famílias, sendo dez adolescentes de ambos os sexos, com idades entre onze a dezoito anos. Para análise do material empírico, recorreu-se à análise temática indutiva. Resultados: Emergiram duas categorias temáticas, responsabilidade da família: alimentação e adolescência; riscos à saúde: o sedentarismo associado ao distanciamento social. Evidenciou-se que o distanciamento físico favoreceu o aumento da tolerância dos pais em relação ao tempo em que os filhos ficaram expostos às telas. Além disso, os pais também influenciaram a prática de hábitos alimentares não saudáveis entre os adolescentes e a manifestação de mudanças no comportamento dos filhos. Conclusão: Assim, a estrutura, composição e comunicação e, consequentemente, interação familiar, são componentes que se articulam diretamente com os hábitos alimentares e comportamentos sedentários pelos adolescentes. Sendo imprescindível uma intervenção multiprofissional e acompanhamento contínuo no que se refere ao desenvolvimento e retorno dos adolescentes às atividades presenciais.
Potential of Organization of Turkic States in the International System: Promises and Vulnerabilities
Authors: Koçak Muhammet
Published: December 2023

This article examines the Organization of Turkic States (OTS) in the context of the complex geopolitical landscape of Central Asia and the Caucasus. It aims to assess its potential in economic, strategic, and social dimensions within international and regional systems. Employing an eclectic theoretical framework, this study looks at the multifaceted factors that influence the OTS’s potential to foster cooperation and solidarity among Turkic states. The research examines this mechanism in interconnected strategic, economic, and social structures. The strategic dimension encompasses defense alliances and security arrangements, the economic dimension focuses on cooperation and trade, and the social dimension emphasizes cultural and normative aspects. Overall, one of the most important rationales behind the OTS is the idea that it serves as a cohesive force enabling Turkic states to navigate the intricate geopolitical environment of Central Asia and the Caucasus where multiple great powers are vying for influence. This research sheds light on the nuanced interplay of economic, strategic, and soft power activities, providing valuable insights into the OTS’s position and influence in Central Asia and the Caucasus. Aside from its contribution to the literature, this article also aims to assist policymakers wishing to explore the potential and limitations of OTS’s increasing influence.
3D digital technology – A revolution in endodontics
Authors: Gollamudi Ramya , Chaudhary Devendra , Sidana Jasmohan Singh , Dev Beena J , Daniel Nikhil , Yadav Divya
Published: December 2023

Cone-beam computed tomography (CBCT) is a formidable technology that has made its place in the field of dentistry for over a decade now. The advancement in the field of digital 3D modeling and 3D printing combined with CBCT is transforming the way dental treatments are planned and executed. The aim of this article is to bring out various modalities of CBCT, its integration of 3D printing, and 3D virtual planning with a paradigm shift in the method of diagnosis, treatment planning, and execution.
Evaluation of natural head position changes in the treatment of class II malocclusions with functional appliance
Authors: Yassaei Soghra , Vakil Fateme , Nasr Nazanin
Published: December 2023

Objectives:

The position of the head to the neck is affected by various factors, including respiratory tract features, temporomandibular joint disorders, type of occlusion, and physical aspects. In addition, malocclusion can negatively affect the head-neck position. The present study aimed to evaluate changes in natural head position following treatment of Class II malocclusions with Farmand II functional appliance.

Material and Methods:

The present study was implemented as a historical cohort with the before and after design. Pre- and post-treatment lateral cephalometric radiographs of 33 skeletal Class II patients treated with the Farmand II functional appliance were included in the present study. The cephalometric analysis was done by tracing the related lines and landmarks. Facial angles and angles determining the natural head and neck position including craniohorizontal, craniocervical, craniovertical (CCV), and cervical curvature (CCI) were measured. Collected data were entered in Statistical Package for the Social Sciences (SPSS)-version 17 software. Paired t-test was used to evaluate the changes in cephalometric indices before and after treatment. P < 0.05 was defined to be statistically significant for all the tests.

Results:

The results of the present study, showed a significant reduction in the mean values of A point, nasion, B point (ANB) angle and angle of convexity following treatment (P < 0.001); also, Sella-nasion/Odontoid process tangent (SN/OPT) angle showed a substantial decrease in treated patients (P = 0.033), which is indicative of head flexion following treatment. Moreover, the decrease in the mean values of the craniocervical angles, including True vertical line (NL/VER) and Sella-nasion/true vertical line (SN/VER), suggests downward bending of the head in patients treated with this appliance (P-values of 0.029 and 0.012, respectively). The odontoid process tangent/cervical vertebra tangent (OPT/CVT) angle showed an insignificant increase (P = 0.260).

Conclusion:

The present study showed that patients treated with the Farmand II functional appliance tend to flex their heads and maintain their cervical vertebra in a more upright and straight position.
3D wrist imaging – Is it time for superman to retire?
Authors: Mørup Svea Deppe , Mussmann Bo , Pedersen Malene Roland Vils , Rasumssen Lykke Moseholm , Gaarde Katrine , Jensen Janni
Published: December 2023

Objectives:

Computed tomography (CT) of the wrist may be challenged, due to patients’ inability to extend the arm for a “Superman pose” resulting in increased radiation dose due to scatter. Alternative positions and less dose administering modalities such as 3D Cone-beam CT (CBCT) and single-shot CT could be considered. This phantom study aimed to estimate scatter radiation dose in different phantom positions using helical and single-shot CT and 3D CBCT.

Material and Methods:

Wireless electronic dosimeters attached to the head and chest of an anthropomorphic phantom in various clinically relevant positions were used to measure scatter radiation. In helical CT, the following positions were used: Superman pose, semi-superman pose, wrist on the abdomen, and single-shot CT with the patient sitting in front of and behind the gantry. In 3D CBCT, the phantom was in a supine position with the arm extended laterally.

Results:

Helical CT using the Superman pose resulted in a total scattered radiation dose of 64.8 µGy. The highest total dose (269.7 µGy) was obtained with the wrist positioned on the abdomen while the lowest total dose was achieved in single-shot CT with the phantom sitting behind the gantry with the forearm placed inside the gantry (3.2 µGy). The total dose in 3D CBCT was 171.1 µGy.

Conclusion:

The commonly used semi-superman and wrist-on-abdomen positions in CT administer the highest scattered doses and should be avoided when either single-shot CT or 3D CBCT is available. Radiographers should carefully consider alternatives when a patient referred for wrist CT cannot comply with the Superman position.
Retrospective analysis of patients diagnosed with severe hyponatraemia in the emergency department
Authors: SENDAG REFIKA , BABUS SEYRAN BOZKURT , KÖSE ATAMAN , TEMEL GÜLHAN OREKICI , TURGUTALP KENAN , TENEKECI SEVCAN
Published: December 2023

Background

. We investigated the demographic, aetiological and clinical characteristics of patients who presented to the emergency department and had severe hyponatraemia.

Methods

. We retrospectively evaluated 1171 patients who presented to the emergency department and were diagnosed with severe hyponatraemia.

Results

. Over half the patients studied were women (53.5%). The mean age of women was higher (p<0.0001). The most common complaint was dyspnoea (16.8%) and 42.5% of the patients had an oral intake disorder. In addition, 22.3% of the patients were on loop diuretics. About 76.3% of patients were conscious, and the mean sodium level of these patients was higher than the mean sodium level of those who responded to verbal and painful stimuli (p<0.001). Severe isolated hyponatraemia was observed in 61.8% of the patients. The mortality rate was 12.8%. There was no difference between the mean sodium levels of the deceased and discharged patients (p=1.0). The mortality rate was higher in patients who had a history of cirrhosis and cancer, but was lower in patients with a history of coronary artery disease (p=0.0002, p<0.0001 and p=0.04, respectively).

Conclusion

. Severe hyponatraemia was more prevalent in women, serum sodium levels were higher in conscious patients, and the mortality rate was higher in patients who had a history of cirrhosis and cancer. We found that the mean serum sodium levels did not help in distinguishing between the deceased and discharged patients.
Metodología para control de calidad de datos de temperatura del mar, Pacífico colombiano
Authors: Castañeda Rodriguez Diana Paulina , Bernal Suárez Néstor Ricardo , Ortiz Martínez Ruby Viviana , Gutiérrez Sarmiento Martha Cecilia , Marriaga Rocha Leonardo
Published: December 2023

Se describe una propuesta metodológica para el control de calidad de datos de Temperatura Superficial del Mar (TSM), compuesta por tres etapas conducentes a la asignación de banderas de calidad recomendadas por el programa para el Intercambio Internacional de Datos e Información Oceanográfica (IODE); en estas etapas se adoptaron buenas prácticas de referentes nacionales e internacionales, así como criterios estadísticos, oceanográficos y climatológicos. Durante la aplicación de la metodología en datos de TSM recopilados porla Dirección General Marítima en cruceros oceanográficos del Pacífico colombiano en el marco del programa del Estudio Regional del Fenómeno El Niño (ERFEN), se evaluó la coherencia temporal y espacial de los datos y se identificaron comportamientos atípicos teniendoen cuenta la influencia de El Niño/La Niña sobre los rangos de variabilidad de la TSM, obteniendo como resultado que bajo este esquema 74,09 % de los datos se le asignó la bandera de calidad clasificada como “buena”.
¿Cómo proteger los humedales costeros peruanos? Una respuesta a partir de un modelo conceptual de sus impulsores de cambio
Authors: Gomez Vilca Angelo Jesús , Aponte Héctor , Gonzales Sergio
Published: December 2023

El presente trabajo consistió en determinar el número de Impulsores de cambio (IC) de 24 humedales de la costa peruana a partir una revisión de los artículos científicos publicados entre el año 2000 y 2020, con el uso de las plataformas Science Direct, Google Académico, Scopus y Scielo. Se revisaron 172 artículos científicos, identificando 22 IC. La agricultura, ganadería, introducción de especies y acumulación de escombros fueron los IC directos más frecuentes, mientras que la mala gobernanza fue el IC indirecto más común (38%); el humedal con mayor número de IC fue Los Pantanos de Villa. Se verificó una correlación positiva entre la producción de artículos científicos y el número de IC identificados por humedal. Cuatro grupos de humedales tuvieron alta similitud en sus IC (≥ 50 %), lo que sugiere que las medidas de gestión para hacerles frente a estos impactos sean similares. La coaparición de los IC se conformó en dos grupos con una similitud ≥ al 50%. Se elaboró un modelo conceptual de las interacciones entre IC y sus efectos sobre los procesos ecológicos. La información de este trabajo puede servir para guiar medidas para la mejor gestión de estos ecosistemas en la costa peruana.
Mortalidad masiva del pez tamboril Canthigaster rostrata (Tetraodontiformes: Tetraodontidae) en el Caribe Sur de Costa Rica con observaciones sobre su relación con cambios ambientales marinos.
Authors: Ramírez-Vargas Marco Antonio , Castillo-Chinchilla Maikol , Piedra-Castro Lilliana
Published: December 2023

Durante el año 2020, ocurrió un evento de mortalidad masiva del pez Canthigaster rostrata en el Refugio de Vida Silvestre Gandoca-Manzanillo (REGAMA), en la costa Caribe Sur de Costa Rica; mismo que se suma a otros reportes en diferentes localidades del Gran Caribe. Este se compuso de individuos con longitud estándar y peso promedio de 40,59 ± 3,82 mm y 0,91 ± 0,27 g respectivamente, considerados adultos prontos a alcanzar la madurez sexual. La distribución de tallas fue similar a la observada durante otro evento de mortalidad en el 2017, también en el REGAMA. La comparación con otros eventos en la región, permitió determinar que el aumento en la temperatura superficial del mar parece ser el factor detonante del fenómeno. Estas anomalías en la temperatura tienen la capacidad de incidir en las comunidades de peces provocando estrés térmico y afectación del metabolismo. Se destaca la necesidad que tiene Costa Rica y la región de desarrollar y mantener monitoreo participativos que funcionen como plataforma para el rastreo, mapeo y análisis de las anomalías térmicas y de otras variables oceanográficas que puedan afectar la biodiversidad de los ecosistemas marinos.
Micobiota endófita de Thalassia testudinum König en el Caribe colombiano
Authors: Castro González Maribeb
Published: December 2023

Se evaluó la presencia de hongos endófitos en hojas de Thalassia testudinum a través del secuenciamiento de la región ITS encontrando abundancias de Ascomicetos (35,9 %), Basidiomicetos (2,9 %) y un alto porcentaje (60 %) de secuencias no clasificadas dada la limitada base de datos existentes. La familia más abundante fue Saccharomycetales _fam_Incertae_sedis (Ascomicetos) aunque un gran número de secuencias no pudieron ser identificadas a este nivel, mientras que, en Basidiomicetos la familia más abundante fue Sporidiobolaceae. A nivel de género se observó variedad tanto de Ascomicetos como de Basidiomicetos, incluyendo levaduras y hongos filamentosos y se evidenció diferencia entre áreas de muestreo. Los resultados demuestran que la microbiota endófita de T. testudinum es muy variada e incluye géneros no descritos previamente de los que se desconoce su interacción con el hospedero y su papel en el mantenimiento de la salud de estas praderas de pastos marinos en el Caribe Colombiano.
Confirmación de la presencia y distribución latitudinal de Echinorhinus cookei en la zona costera del Pacífico Oriental Tropical
Authors: Navia Andrés Felipe , Tobón-López Alexander , Segura Carlos Eduardo , Córdoba Diego Fernando , Amariles Diego Fernando , Caicedo Julián Alejandro , Mejía-Falla Paola Andrea
Published: December 2023

La distribución conocida del tiburón espinoso negro del Pacífico Echinorhinus cookei en el Pacífico Oriental se extiende desde Oregón (EE.UU.) hasta Costa Rica y desde Ecuador a Chile, con vacíos en las zonas costeras de Panamá y Colombia. El presente documento confirma la presencia de E. cookei en el Pacífico costero colombiano y verifica que su distribución es continua a lo largo del rango latitudinal en el Pacífico Oriental.
UJI KUALITAS SINTESIS KARBON AKTIF DARI PELEPAH AREN TERAKTIVASI ASAM FOSFAT
Authors: Neneng Purnamawati
Published: December 2023

Palm fronds which contain a lot of cellulose have the potential to be used as active carbon. There are three stages in making activated carbon from sugar palm fronds, namely the dehydration process, carbonization process, and activation process using phosphoric acid activator (H3PO4) with a concentration of 1M at temperatures of 450°C and 600°C. The aim of this research is to determine the effect of carbonization temperature on the quality of activated carbon from sugar palm fronds. The activated carbon quality tests carried out include yield tests, water content tests, ash content tests and iodine absorption tests. The results of testing the quality of activated carbon showed that optimal conditions were at a temperature of 450°C with a yield of 22.189%, a water content of 2.562%, a total ash content of 5.86% and an iodine absorption capacity of 81.423 mg/g.