bluuebunny/crossref_metadata_2025 huggingface dataset:
Using Deep Learning for an Analysis of Atmospheric Rivers in a High‐Resolution Large Ensemble Climate Data Set
Authors: Higgins Timothy B. , Subramanian Aneesh C. , Graubner Andre , Kapp‐Schwoerer Lukas , Watson Peter A. G. , Sparrow Sarah , Kashinath Karthik , Kim Sol , Delle Monache Luca , Chapman Will
Published: April 2023

There is currently large uncertainty over the impacts of climate change on precipitation trends over the US west coast. Atmospheric rivers (ARs) are a significant source of US west coast precipitation and trends in ARs can provide insight into future precipitation trends. There are already a variety of different methods used to identify ARs, but many are used in contexts that are often difficult to apply to large climate datasets due to their computational cost and requirement of integrated vapor transport as an input variable, which can be expensive to compute in climate models at high temporal frequencies. Using deep learning (DL) to track ARs is a unique approach that can alleviate some of the computational challenges that exist in more traditional methods. However, some questions still remain regarding its flexibility and robustness. This research investigates the consistency of a DL methodology of tracking ARs with more established algorithms to demonstrate its high‐level performance for future studies.
As the Arctic Warms, These Rivers Are Slowing Down
Authors: Beurteaux Danielle
Published: April 2023

The Arctic is warming up, but instead of large rivers migrating faster, they’re actually slowing down because of shrubification.
Upstream Proton Cyclotron Waves at Mars During the Passage of ICMEs
Authors: Zhao Dan , Guo Jianpeng , Lin Haibo , Mazelle Christian , He Linxia , Meng Weiduo , Chen Yan , Kong LingGao , Wei Yong , Liu Libo
Published: April 2023

The presence of proton cyclotron waves (PCWs) upstream from Mars indicates picked up protons originating from the Martian extended hydrogen (H) exosphere by the solar wind and loss of H to interplanetary space. The occurrences and properties of PCWs associated with normal solar wind conditions have been extensively investigated since their initial detection about 32 years ago, but little attention has been paid to PCW activity under extreme space weather conditions. Here we characterize PCWs during the passage of interplanetary coronal mass ejections (ICMEs) through the Martian environment using the measurements by the Mars Atmosphere and Volatile EvolutioN spacecraft from December 2014 to February 2019. It is found that PCWs occur more frequently during perihelion periods (Ls = 180°–360°) than during aphelion periods (Ls = 0°–180°) for disturbed times of ICME passage. In the perihelion season, the PCW occurrence rate is increased on average by a factor of about 2 during the ICME phase relative to the pre‐ICME and post‐ICME phases. Moreover, PCWs reveal more pronounced wave characteristics with larger amplitudes, higher ellipticity, and smaller propagation angle with respect to the ambient magnetic field direction during the ICME phase.
Cooking methods and kitchen ventilation availability, usage, perceived performance and potential in Canadian homes
Authors: Sun Liu , Singer Brett C.
Published: April 2023

Background

Cooking is a substantial contributor to air pollutant exposures in many residences. Effective use of kitchen ventilation can mitigate exposure; however, information on its availability, usage, and potential to increase its use across the population has been limited.

Objective

This study aimed to obtain nationally representative information on cooking methods, kitchen ventilation availability and usage, and the potential for education to increase effective usage.

Methods

An online survey was sent to a representative sample of Canadian homes to collect data on cooking methods, the presence and use of mechanical kitchen ventilation devices, perceived device performance, and willingness to implement mitigation strategies. Responses were weighted to match key demographic factors and analyzed using non-parametric statistics.

Results

Among the 4500 respondents, 90% had mechanical ventilation devices over the cooktop (66% of which were vented to the outside), and 30% reported regularly using their devices. Devices were used most often for deep-frying, followed by stir-frying, sautéing or pan-frying, indoor grilling, boiling or steaming. Almost half reported rarely or never using their ventilation devices during baking or oven self-cleaning. Only 10% were fully satisfied with their devices. More frequent use was associated with the device being vented to the outdoors, having more than two speed settings, quiet operation if only one speed, covering over half of the cooktop, and higher perceived effectiveness. After being informed of the benefits of kitchen ventilation, 64% indicated they would consider using their devices more often, preferentially using back burners with ventilation, and/or using higher ventilation device settings when needed.

Impact

This study provides population-representative data on the most used cooking methods, kitchen ventilation availability and usage, and influencing factors in Canadian homes. Such data are needed for exposure assessments and evaluating the potential to mitigate cooking-related pollutant exposures via more effective use of kitchen ventilation. The data can be reasonably extrapolated to the United States, given the similarities in residential construction practices and cultural norms between the two countries.
Energy drinks: effects on pediatric 24-h ambulatory blood pressure monitoring. A randomized trial
Authors: Oberhoffer Felix S. , Dalla-Pozza Robert , Jakob André , Haas Nikolaus A. , Mandilaras Guido , Li Pengzhu
Published: April 2023

Background

Energy drinks (EDs) are popular beverages among minors. To date, clinical studies investigating ED-induced effects on the pediatric cardiovascular system are sparse. This study aimed to investigate the effects of a single, bodyweight-adjusted ED dosage on 24-h ambulatory blood pressure monitoring (ABPM) in healthy children and adolescents.

Methods

This study was a randomized, single-blind, placebo-controlled, crossover clinical trial. Study participants received a single, bodyweight-adjusted ED amount or a placebo drink on 2 consecutive days at similar morning hours. Twenty-four-hour ABPM was assessed via an automated oscillometric blood pressure device after beverage consumption on both study days.

Results

A total of 17 healthy children and teenagers (13.90 (12.29–17.89) years) were included in the final analysis. The ED consumption led, compared to the placebo intake, to a significantly higher 24-h systolic (115.90 (110.22–118.04) vs. 110.64 (108.09–115.45) mmHg, p = 0.013) and diastolic blood pressure (66.08 (64.20–68.32) vs. 62.63 (61.40–66.46) mmHg, p = 0.005).

Conclusions

The single, bodyweight-adjusted ED consumption is linked with a significantly higher systolic as well as diastolic 24-h blood pressure in healthy children and adolescents. Minors, particularly those with an increased cardiovascular morbidity, should be discouraged from drinking EDs.

Impact

Energy drinks (EDs) are consumed by many children and teenagers. While adverse cardiovascular events after ED consumption were reported in the literature, the effects of these beverages on the pediatric 24-h blood pressure profile have not been systematically evaluated yet. In our manuscript, we demonstrate for the first time that acute ED consumption is associated with a significantly higher 24-h systolic blood pressure and diastolic blood pressure in healthy minors.
Biogeographic patterns of biosynthetic potential and specialized metabolites in marine sediments
Authors: Chase Alexander B , Bogdanov Alexander , Demko Alyssa M , Jensen Paul R
Published: April 2023

While the field of microbial biogeography has largely focused on the contributions of abiotic factors to community patterns, the potential influence of biotic interactions in structuring microbial communities, such as those mediated by the production of specialized metabolites, remains largely unknown. Here, we examined the relationship between microbial community structure and specialized metabolism at local spatial scales in marine sediment samples collected from the Long-Term Ecological Research (LTER) site in Moorea, French Polynesia. By employing a multi-omic approach to characterize the taxonomic, functional, and specialized metabolite composition within sediment communities, we find that biogeographic patterns were driven by local scale processes (e.g., biotic interactions) and largely independent of dispersal limitation. Specifically, we observed high variation in biosynthetic potential (based on Bray-Curtis dissimilarity) between samples, even within 1 m2 plots, that reflected uncharacterized chemical space associated with site-specific metabolomes. Ultimately, connecting biosynthetic potential to community metabolomes facilitated the in situ detection of natural products and revealed new insights into the complex metabolic dynamics associated with sediment microbial communities. Our study demonstrates the potential to integrate biosynthetic genes and metabolite production into assessments of microbial community dynamics.
Reprogramming the metabolism of an acetogenic bacterium to homoformatogenesis
Authors: Moon Jimyung , Schubert Anja , Waschinger Lara M , Müller Volker
Published: April 2023

Methyl groups are abundant in anoxic environments and their utilization as carbon and energy sources by microorganisms involves oxidation of the methyl groups to CO2, followed by transfer of the electrons to an acceptor. In acetogenic bacteria, the electron acceptor is CO2 that is reduced to enzyme bound carbon monoxide, the precursor of the carboxyl group in acetate. Here, we describe the generation of a mutant of the acetogen Acetobacterium woodii in which the last step in methyl group oxidation, formate oxidation to CO2 catalyzed by the HDCR enzyme, has been genetically deleted. The mutant grew on glycine betaine as methyl group donor, and in contrast to the wild type, formed formate alongside acetate, in a 1:2 ratio, demonstrating that methyl group oxidation stopped at the level of formate and reduced electron carriers were reoxidized by CO2 reduction to acetate. In the presence of the alternative electron acceptor caffeate, CO2 was no longer reduced to acetate, formate was the only product and all the carbon went to formate. Apparently, acetogenesis was not required to sustain formatogenic growth. This is the first demonstration of a genetic reprogramming of an acetogen into a formatogen that grows by homoformatogenesis from methyl groups. Formate production from methyl groups is not only of biotechnological interest but also for the mechanism of electron transfer in syntrophic interactions in anoxic environments.
A bibliometric analysis of ferroptosis, necroptosis, pyroptosis, and cuproptosis in cancer from 2012 to 2022
Authors: Miao Yan-Dong , Quan Wuxia , Dong Xin , Gan Jian , Ji Cui-Feng , Wang Jiang-Tao , Zhang Fang
Published: April 2023

This study aims to visualize research hotspots and trends of “ferroptosis in cancer”, “necroptosis in cancer”, “pyroptosis in cancer”, and “cuproptosis in cancer” through a bibliometric analysis to facilitate understanding of future developments in basic and clinical research and to provide a new perspective on cancer treatment. From January 1, 2012 to October 31, 2022, in the field of “ferroptosis in cancer”, a total of 2467 organizations from 79 different countries published 3302 articles. 2274 organizations from 72 different countries published 2233 articles in the field of “ necroptosis in cancer”. 1366 institutions from 58 different countries contributed 1445 publications in the field of “pyroptosis in cancer”. In the field of “ cuproptosis in cancer”, the number of articles published in the last 10 years is relatively low, with a total of 109 articles published by 116 institutions from four different countries. In the field of “ferroptosis in cancer”, Tang Daolin had published 66 documents, ranked the first, while Dixon SJ is the most cited author, cited 3148 times; In the fields of “necroptosis in cancer”, Vandenabeele peter had published 35 papers and Degterev had been cited 995 times, ranked the first, respectively; Kanneganti thirumala-devi had published 24 papers, is the highest number of publications in the fields of “pyroptosis in cancer”, while Shi JJ was the most cited author with being cited 508 times. Both Huang Yan and Wang Tao published three papers and tied for first place and Tsvetkov p ranks first with being cited 107 times in “cuproptosis in cancer”. “Cell”, “Cell”, “Nature”, and “Science” was the most frequently co-cited journal on “ferroptosis in cancer”, “necroptosis in cancer”, “pyroptosis in cancer”, and “cuproptosis in cancer”, respectively. Further exploration of inhibitors of different Programmed cell death (PCD) and their targeted therapies are potential treatment options for cancer, but more direct clinical evidence as well as higher level clinical trials remain to be explored. Further clarification of the mechanisms of crosstalk between these PCDs may provide effective cancer treatments. And the role of different types of PCDs, especially the novel ones discovered, in cancer can be expected to remain a hot topic of research in the cancer field for quite some time to come.
Ferroptosis landscape in prostate cancer from molecular and metabolic perspective
Authors: Liang Jiaming , Liao Yihao , Wang Pu , Yang Kun , Wang Youzhi , Wang Keke , Zhong Boqiang , Zhou Diansheng , Cao Qian , Li Junbo , Zhao Yang , Jiang Ning
Published: April 2023

Prostate cancer is a major disease that threatens men’s health. Its rapid progression, easy metastasis, and late castration resistance have brought obstacles to treatment. It is necessary to find new effective anticancer methods. Ferroptosis is a novel iron-dependent programmed cell death that plays a role in various cancers. Understanding how ferroptosis is regulated in prostate cancer will help us to use it as a new way to kill cancer cells. In this review, we summarize the regulation and role of ferroptosis in prostate cancer and the relationship with AR from the perspective of metabolism and molecular pathways. We also discuss the feasibility of ferroptosis in prostate cancer treatment and describe current limitations and prospects, providing a reference for future research and clinical application of ferroptosis.
Trim-Away ubiquitinates and degrades lysine-less and N-terminally acetylated substrates
Authors: Kiss Leo , Rhinesmith Tyler , Luptak Jakub , Dickson Claire F. , Weidenhausen Jonas , Smyly Shannon , Yang Ji-Chun , Maslen Sarah L. , Sinning Irmgard , Neuhaus David , Clift Dean , James Leo C.
Published: April 2023

TRIM proteins are the largest family of E3 ligases in mammals. They include the intracellular antibody receptor TRIM21, which is responsible for mediating targeted protein degradation during Trim-Away. Despite their importance, the ubiquitination mechanism of TRIM ligases has remained elusive. Here we show that while Trim-Away activation results in ubiquitination of both ligase and substrate, ligase ubiquitination is not required for substrate degradation. N-terminal TRIM21 RING ubiquitination by the E2 Ube2W can be inhibited by N-terminal acetylation, but this doesn’t prevent substrate ubiquitination nor degradation. Instead, uncoupling ligase and substrate degradation prevents ligase recycling and extends functional persistence in cells. Further, Trim-Away degrades substrates irrespective of whether they contain lysines or are N-terminally acetylated, which may explain the ability of TRIM21 to counteract fast-evolving pathogens and degrade diverse substrates.
Piezo2 regulates colonic mechanical sensitivity in a sex specific manner in mice
Authors: Madar Jonathan , Tiwari Namrata , Smith Cristina , Sharma Divya , Shen Shanwei , Elmahdi Alsiddig , Qiao Liya Y.
Published: April 2023

The mechanosensitive ion channel Piezo2 in mucosa and primary afferents transduces colonic mechanical sensation. Here we show that chemogenetic activation or nociceptor-targeted deletion of Piezo2 is sufficient to regulate colonic mechanical sensitivity in a sex dependent manner. Clozapine N-oxide-induced activation of Piezo2;hM3Dq-expressing sensory neurons evokes colonic hypersensitivity in male mice, and causes dyspnea in female mice likely due to effects on lung sensory neurons. Activation of Piezo2-expressing colonic afferent neurons also induces colonic hypersensitivity in male but not female mice. Piezo2 levels in nociceptive neurons are higher in female than in male mice. We also show that Piezo2 conditional deletion from nociceptive neurons increases body weight growth, slows colonic transits, and reduces colonic mechanosensing in female but not male mice. Piezo2 deletion blocks colonic hypersensitivity in male but not female mice. These results suggest that Piezo2 in nociceptive neurons mediates innocuous colonic mechanosensing in female mice and painful sensation in male mice, suggesting a sexual dimorphism of Piezo2 function in the colonic sensory system.
Controlling protein stability with SULI, a highly sensitive tag for stabilization upon light induction
Authors: Mao Miaowei , Qian Yajie , Zhang Wenyao , Zhou Siyu , Wang Zefeng , Chen Xianjun , Yang Yi
Published: April 2023

Optogenetics tools for precise temporal and spatial control of protein abundance are valuable in studying diverse complex biological processes. In the present study, we engineer a monomeric tag of stabilization upon light induction (SULI) for yeast and zebrafish based on a single light-oxygen-voltage domain from Neurospora crassa. Proteins of interest fused with SULI are stable upon light illumination but are readily degraded after transfer to dark conditions. SULI shows a high dynamic range and a high tolerance to fusion at different positions of the target protein. Further studies reveal that SULI-mediated degradation occurs through a lysine ubiquitination-independent proteasome pathway. We demonstrate the usefulness of SULI in controlling the cell cycle in yeast and regulating protein stability in zebrafish, respectively. Overall, our data indicate that SULI is a simple and robust tool to quantitatively and spatiotemporally modulate protein levels for biotechnological or biomedical applications.
Delayed generation of functional virus-specific circulating T follicular helper cells correlates with severe COVID-19
Authors: Yu Meng , Charles Afandi , Cagigi Alberto , Christ Wanda , Österberg Björn , Falck-Jones Sara , Azizmohammadi Lida , Åhlberg Eric , Falck-Jones Ryan , Svensson Julia , Nie Mu , Warnqvist Anna , Hellgren Fredrika , Lenart Klara , Arcoverde Cerveira Rodrigo , Ols Sebastian , Lindgren Gustaf , Lin Ang , Maecker Holden , Bell Max , Johansson Niclas , Albert Jan , Sundling Christopher , Czarnewski Paulo , Klingström Jonas , Färnert Anna , Loré Karin , Smed-Sörensen Anna
Published: April 2023

Effective humoral immune responses require well-orchestrated B and T follicular helper (Tfh) cell interactions. Whether these interactions are impaired and associated with COVID-19 disease severity is unclear. Here, longitudinal blood samples across COVID-19 disease severity are analysed. We find that during acute infection SARS-CoV-2-specific circulating Tfh (cTfh) cells expand with disease severity. SARS-CoV-2-specific cTfh cell frequencies correlate with plasmablast frequencies and SARS-CoV-2 antibody titers, avidity and neutralization. Furthermore, cTfh cells but not other memory CD4 T cells, from severe patients better induce plasmablast differentiation and antibody production compared to cTfh cells from mild patients. However, virus-specific cTfh cell development is delayed in patients that display or later develop severe disease compared to those with mild disease, which correlates with delayed induction of high-avidity neutralizing antibodies. Our study suggests that impaired generation of functional virus-specific cTfh cells delays high-quality antibody production at an early stage, potentially enabling progression to severe disease.
Global scale analysis on the extent of river channel belts
Authors: Nyberg Björn , Henstra Gijs , Gawthorpe Rob L. , Ravnås Rodmar , Ahokas Juha
Published: April 2023

Rivers form channel belts that encompass the area of the river channel and its associated levees, bars, splays and overbank landforms. The channel belt is critical for understanding the physical river evolution through time, predicting river behavior and management of freshwater resources. To date, there is no global-scale, quantitative study of the extent of river channel belts. Here we show, based on a pattern recognition algorithm, the global surface area of channel belts at an approximate 1 km resolution is 30.5 × 105 km2, seven times larger than the extent of river channels. We find 52% of river channels associated with the channel belts have a multi-threaded planform with the remaining 48% being single-threaded by surface area. The global channel belt (GCB) datasets provide new methods for high-resolution global scale landform classifications and for incorporating the channel belt into flood mitigation, freshwater budgets, ecosystem accounting and biogeochemical analyses.
Evaluation of the methane paradox in four adjacent pre-alpine lakes across a trophic gradient
Authors: Ordóñez César , DelSontro Tonya , Langenegger Timon , Donis Daphne , Suarez Ena L. , McGinnis Daniel F.
Published: April 2023

Contrasting the paradigm that methane is only produced in anoxic conditions, recent discoveries show that oxic methane production (OMP, aka the methane paradox) occurs in oxygenated surface waters worldwide. OMP drivers and their contribution to global methane emissions, however, are not well constrained. In four adjacent pre-alpine lakes, we determine the net methane production rates in oxic surface waters using two mass balance approaches, accounting for methane sources and sinks. We find that OMP occurs in three out of four studied lakes, often as the dominant source of diffusive methane emissions. Correlations of net methane production versus chlorophyll-a, Secchi and surface mixed layer depths suggest a link with photosynthesis and provides an empirical upscaling approach. As OMP is a methane source in direct contact with the atmosphere, a better understanding of its extent and drivers is necessary to constrain the atmospheric methane contribution by inland waters.
Native doublet microtubules from Tetrahymena thermophila reveal the importance of outer junction proteins
Authors: Kubo Shintaroh , Black Corbin S. , Joachimiak Ewa , Yang Shun Kai , Legal Thibault , Peri Katya , Khalifa Ahmad Abdelzaher Zaki , Ghanaeian Avrin , McCafferty Caitlyn L. , Valente-Paterno Melissa , De Bellis Chelsea , Huynh Phuong M. , Fan Zhe , Marcotte Edward M. , Wloga Dorota , Bui Khanh Huy
Published: April 2023

Cilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central singlets and nine outer doublet microtubules. Cryo-electron microscopy-based studies have revealed a complex network inside the lumen of both tubules composed of microtubule-inner proteins (MIPs). However, the functions of most MIPs remain unknown. Here, we present single-particle cryo-EM-based analyses of the Tetrahymena thermophila native doublet microtubule and identify 42 MIPs. These data shed light on the evolutionarily conserved and diversified roles of MIPs. In addition, we identified MIPs potentially responsible for the assembly and stability of the doublet outer junction. Knockout of the evolutionarily conserved outer junction component CFAP77 moderately diminishes Tetrahymena swimming speed and beat frequency, indicating the important role of CFAP77 and outer junction stability in cilia beating generation and/or regulation.
Next-generation large-scale binary protein interaction network for Drosophila melanogaster
Authors: Tang Hong-Wen , Spirohn Kerstin , Hu Yanhui , Hao Tong , Kovács István A. , Gao Yue , Binari Richard , Yang-Zhou Donghui , Wan Kenneth H. , Bader Joel S. , Balcha Dawit , Bian Wenting , Booth Benjamin W. , Coté Atina G. , de Rouck Steffi , Desbuleux Alice , Goh Kah Yong , Kim Dae-Kyum , Knapp Jennifer J. , Lee Wen Xing , Lemmens Irma , Li Cathleen , Li Mian , Li Roujia , Lim Hyobin Julianne , Liu Yifang , Luck Katja , Markey Dylan , Pollis Carl , Rangarajan Sudharshan , Rodiger Jonathan , Schlabach Sadie , Shen Yun , Sheykhkarimli Dayag , TeeKing Bridget , Roth Frederick P. , Tavernier Jan , Calderwood Michael A. , Hill David E. , Celniker Susan E. , Vidal Marc , Perrimon Norbert , Mohr Stephanie E.
Published: April 2023

Generating reference maps of interactome networks illuminates genetic studies by providing a protein-centric approach to finding new components of existing pathways, complexes, and processes. We apply state-of-the-art methods to identify binary protein-protein interactions (PPIs) forDrosophila melanogaster. Four all-by-all yeast two-hybrid (Y2H) screens of > 10,000Drosophilaproteins result in the ‘FlyBi’ dataset of 8723 PPIs among 2939 proteins. Testing subsets of data from FlyBi and previous PPI studies using an orthogonal assay allows for normalization of data quality; subsequent integration of FlyBi and previous data results in an expanded binaryDrosophilareference interaction network, DroRI, comprising 17,232 interactions among 6511 proteins. We use FlyBi data to generate an autophagy network, then validate in vivo using autophagy-related assays. Thedeformed wings(dwg) gene encodes a protein that is both a regulator and a target of autophagy. Altogether, these resources provide a foundation for building new hypotheses regarding protein networks and function.
Long-chain anionic surfactants enabling stable perovskite/silicon tandems with greatly suppressed stress corrosion
Authors: Wang Xinlong , Ying Zhiqin , Zheng Jingming , Li Xin , Zhang Zhipeng , Xiao Chuanxiao , Chen Ying , Wu Ming , Yang Zhenhai , Sun Jingsong , Xu Jia-Ru , Sheng Jiang , Zeng Yuheng , Yang Xi , Xing Guichuan , Ye Jichun
Published: April 2023

Despite the remarkable rise in the efficiency of perovskite-based solar cells, the stress-induced intrinsic instability of perovskite active layers is widely identified as a critical hurdle for upcoming commercialization. Herein, a long-alkyl-chain anionic surfactant additive is introduced to chemically ameliorate the perovskite crystallization kinetics via surface segregation and micellization, and physically construct a glue-like scaffold to eliminate the residual stresses. As a result, benefiting from the reduced defects, suppressed ion migration and improved energy level alignment, the corresponding unencapsulated perovskite single-junction and perovskite/silicon tandem devices exhibit impressive operational stability with 85.7% and 93.6% of their performance after 3000 h and 450 h at maximum power point tracking under continuous light illumination, providing one of the best stabilities to date under similar test conditions, respectively.
Dynamic machine vision with retinomorphic photomemristor-reservoir computing
Authors: Tan Hongwei , van Dijken Sebastiaan
Published: April 2023

Dynamic machine vision requires recognizing the past and predicting the future of a moving object based on present vision. Current machine vision systems accomplish this by processing numerous image frames or using complex algorithms. Here, we report motion recognition and prediction in recurrent photomemristor networks. In our system, a retinomorphic photomemristor array, working as dynamic vision reservoir, embeds past motion frames as hidden states into the present frame through inherent dynamic memory. The informative present frame facilitates accurate recognition of past and prediction of future motions with machine learning algorithms. This in-sensor motion processing capability eliminates redundant data flows and promotes real-time perception of moving objects for dynamic machine vision.
Global patterns and edaphic-climatic controls of soil carbon decomposition kinetics predicted from incubation experiments
Authors: Xiang Daifeng , Wang Gangsheng , Tian Jing , Li Wanyu
Published: April 2023

Knowledge about global patterns of the decomposition kinetics of distinct soil organic matter (SOM) pools is crucial to robust estimates of land-atmosphere carbon fluxes under climate change. However, the current Earth system models often adopt globally-consistent reference SOM decomposition rates (kref), ignoring effects from edaphic-climate heterogeneity. Here, we compile a comprehensive set of edaphic-climatic and SOM decomposition data from published incubation experiments and employ machine-learning techniques to develop models capable of predicting the expected sizes and kref of multiple SOM pools (fast, slow, and passive). We show that soil texture dominates the turnover of the fast pools, whereas pH predominantly regulates passive SOM decomposition. This suggests that pH-sensitive bacterial decomposers might have larger effects on stable SOM decomposition than previously believed. Using these predictive models, we provide a 1-km resolution global-scale dataset of the sizes and kref of these SOM pools, which may improve global biogeochemical model parameterization and predictions.
Transcriptomics of Hirschsprung disease patient-derived enteric neural crest cells reveals a role for oxidative phosphorylation
Authors: Li Zhixin , Lui Kathy Nga-Chu , Lau Sin-Ting , Lai Frank Pui-Ling , Li Peng , Chung Patrick Ho-Yu , Wong Kenneth Kak-Yuen , Tam Paul Kwong-Hing , Garica-Barcelo Maria-Mercedes , Hui Chi-Chung , Sham Pak Chung , Ngan Elly Sau-Wai
Published: April 2023

Hirschsprung disease is characterized by the absence of enteric neurons caused by the defects of enteric neural crest cells, leading to intestinal obstruction. Here, using induced pluripotent stem cell-based models of Hirschsprung and single-cell transcriptomic analysis, we identify a gene set of 118 genes commonly dysregulated in all patient enteric neural crest cells, and suggest HDAC1 may be a key regulator of these genes. Furthermore, upregulation of RNA splicing mediators and enhanced alternative splicing events are associated with severe form of Hirschsprung. In particular, the higher inclusion rate of exon 9 in PTBP1 and the perturbed expression of a PTBP1-target, PKM, are significantly enriched in these patient cells, and associated with the defective oxidative phosphorylation and impaired neurogenesis. Hedgehog-induced oxidative phosphorylation significantly enhances the survival and differentiation capacity of patient cells. In sum, we define various factors associated with Hirschsprung pathogenesis and demonstrate the implications of oxidative phosphorylation in enteric neural crest development and HSCR pathogenesis.
Pyro-layered heterostructured nanosheet membrane for hydrogen separation
Authors: Wang Ruoxin , Qian Jianhao , Chen Xiaofang , Low Ze-Xian , Chen Yu , Ma Hongyu , Wu Heng-An , Doherty Cara M. , Acharya Durga , Xie Zongli , Hill Matthew R. , Shen Wei , Wang Fengchao , Wang Huanting
Published: April 2023

Engineering different two-dimensional materials into heterostructured membranes with unique physiochemical properties and molecular sieving channels offers an effective way to design membranes for fast and selective gas molecule transport. Here we develop a simple and versatile pyro-layering approach to fabricate heterostructured membranes from boron nitride nanosheets as the main scaffold and graphene nanosheets derived from a chitosan precursor as the filler. The rearrangement of the graphene nanosheets adjoining the boron nitride nanosheets during the pyro-layering treatment forms precise in-plane slit-like nanochannels and a plane-to-plane spacing of ~3.0 Å, thereby endowing specific gas transport pathways for selective hydrogen transport. The heterostructured membrane shows a high H2permeability of 849 Barrer, with a H2/CO2selectivity of 290. This facile and scalable technique holds great promise for the fabrication of heterostructures as next-generation membranes for enhancing the efficiency of gas separation and purification processes.
Dynamic fluctuations in a bacterial metabolic network
Authors: Bi Shuangyu , Kargeti Manika , Colin Remy , Farke Niklas , Link Hannes , Sourjik Victor
Published: April 2023

The operation of the central metabolism is typically assumed to be deterministic, but dynamics and high connectivity of the metabolic network make it potentially prone to generating fluctuations. However, time-resolved measurements of metabolite levels in individual cells that are required to characterize such fluctuations remained a challenge, particularly in small bacterial cells. Here we use single-cell metabolite measurements based on Förster resonance energy transfer, combined with computer simulations, to explore the real-time dynamics of the metabolic network of Escherichia coli. We observe that steplike exposure of starved E. coli to glycolytic carbon sources elicits large periodic fluctuations in the intracellular concentration of pyruvate in individual cells. These fluctuations are consistent with predicted oscillatory dynamics of E. coli metabolic network, and they are primarily controlled by biochemical reactions around the pyruvate node. Our results further indicate that fluctuations in glycolysis propagate to other cellular processes, possibly leading to temporal heterogeneity of cellular states within a population.
Brainstem neuromelanin and iron MRI reveals a precise signature for idiopathic and LRRK2 Parkinson’s disease
Authors: Martínez Martín , Ariz Mikel , Alvarez Ignacio , Castellanos Gabriel , Aguilar Miquel , Hernández-Vara Jorge , Caballol Núria , Garrido Alicia , Bayés Àngels , Vilas Dolores , Marti Maria Jose ,   the Catalonian Neuroimaging Parkinson’s disease Consortium, Pascual-Sedano Berta , Marin Juan , Avila Asuncion , Buongiorno Mariateresa , Tartari Juan Pablo , Puente Victor , Ezquerra Mario , Valldeoriola Francesc , Compta Yaroslau , Tolosa Eduard , Pont Claustre , Pastor Pau , de Solórzano Carlos Ortiz , Pastor Maria A.
Published: April 2023

Neuromelanin (NM) loss in substantia nigra pars compacta (SNc) and locus coeruleus (LC) reflects neuronal death in Parkinson’s disease (PD). Since genetically-determined PD shows varied clinical expressivity, we wanted to accurately quantify and locate brainstem NM and iron, to discover whether specific MRI patterns are linked to Leucine-rich repeat kinase 2 G2019S PD (LRRK2-PD) or idiopathic Parkinson’s disease (iPD). A 3D automated MRI atlas-based segmentation pipeline (3D-ABSP) for NM/iron-sensitive MRI images topographically characterized the SNc, LC, and red nucleus (RN) neuronal loss and calculated NM/iron contrast ratio (CR) and normalized volume (nVol). Left-side NM nVol was larger in all groups. PD had lower NM CR and nVol in ventral-caudal SNc, whereas iron increased in lateral, medial-rostral, and caudal SNc. The SNc NM CR reduction was associated with psychiatric symptoms. LC CR and nVol discriminated better among subgroups: LRRK2-PD had similar LC NM CR and nVol as that of controls, and larger LC NM nVol and RN iron CR than iPD. PD showed higher iron SNc nVol than controls, especially among LRRK2-PD. ROC analyses showed an AUC > 0.92 for most pairwise subgroup comparisons, with SNc NM being the best discriminator between HC and PD. NM measures maintained their discriminator power considering the subgroup of PD patients with less than 5 years of disease duration. The SNc iron CR and nVol increase was associated with longer disease duration in PD patients. The 3D-ABSP sensitively identified NM and iron MRI patterns strongly correlated with phenotypic PD features.
Topological surface magnetism and Néel vector control in a magnetoelectric antiferromagnet
Authors: Du Kai , Xu Xianghan , Won Choongjae , Wang Kefeng , Crooker Scott A. , Rangan Sylvie , Bartynski Robert , Cheong Sang-Wook
Published: April 2023

Antiferromagnetic states with no stray magnetic fields can enable high-density ultra-fast spintronic technologies. However, the detection and control of antiferromagnetic Néel vectors remain challenging. Linear magnetoelectric antiferromagnets (LMAs) may provide new pathways, but applying simultaneous electric and magnetic fields, necessary to control Néel vectors in LMAs, is cumbersome and impractical for most applications. Herein, we show that Cr2O3, a prototypical room-temperature LMA, carries a topologically-protected surface magnetism in all surfaces, which stems from intrinsic surface electric fields due to band bending, combined with the bulk linear magnetoelectricity. Consequently, bulk Néel vectors with zero bulk magnetization can be simply tuned by magnetic fields through controlling the magnetizations associated with the surface magnetism. Our results imply that the surface magnetizations discovered in Cr2O3 should be also present in all LMAs.
Is new dengue vaccine efficacy data a relief or cause for concern?
Authors: Thomas Stephen J.
Published: April 2023

Dengue is a major global public health problem requiring a safe and efficacious vaccine as the foundation of a comprehensive countermeasure strategy. Despite decades of attempts, the world has a single dengue vaccine licensed in numerous countries, but restrictions and conditions of its use have deterred uptake. Recently, clinical efficacy data has been revealed for two additional dengue vaccine candidates and the data appears encouraging. In this perspective I discuss dengue, the complexities of dengue vaccine development, early development setbacks, and how the latest data from the field may be cause for measured optimism. Finally, I provide some perspectives on evaluating dengue vaccine performance and how the pursuit of the perfect dengue vaccine may prevent advancement of vaccines which are good enough.
Optimization of reaction temperature and Ni–W–Mo catalyst soaking time in oil upgrading: application to kinetic modeling of in-situ upgrading
Authors: Abdi-Khanghah Mahdi , Jafari Arezou , Ahmadi Goodarz , Hemmati-Sarapardeh Abdolhossein
Published: April 2023

Decreasing the conventional sources of oil reservoirs attracts researchers’ attention to the tertiary recovery of oil reservoirs, such as in-situ catalytic upgrading. In this contribution, the response surface methodology (RSM) approach and multi-objective optimization were utilized to investigate the effect of reaction temperature and catalysts soaking time on the concentration distribution of upgraded oil samples. To this end, 22 sets of experimental oil upgrading over Ni–W–Mo catalyst were utilized for the statistical modeling. Then, optimization based on the minimum reaction temperature, catalysts soaking time, gas, and residue wt.% was performed. Also, correlations for the prediction of concentration of different fractions (residue, vacuum gas oil (VGO), distillate, naphtha, and gases) as a function of independent factors were developed. Statistical results revealed that RSM model is in good agreement with experimental data and high coefficients of determination (R2 = 0.96, 0.945, 0.97, 0.996, 0.89) are the witness for this claim. Finally, based on multi-objective optimization, 378.81 °C and 17.31 h were obtained as the optimum upgrading condition. In this condition, the composition of residue, VGO, distillate, naphtha, and gases are 6.798%, 39.23%, 32.93%, 16.865%, and 2.896%, respectively, and the optimum condition is worthwhile for the pilot and industrial application of catalyst injection during in-situ oil upgrading.
Short-term exposure to some heavy metals carried with PM10 and cardiovascular system biomarkers during dust storm
Authors: Badeenezhad Ahmad , Parseh Iman , Veisi Ali , Rostami Saeid , Ghelichi-Ghojogh Mousa , Badfar Gholamreza , Abbasi Fariba
Published: April 2023

This study aimed to evaluate the effect of short-term exposure to heavy metals (HM) extracted from PM10 on CB in workers’ population in an outdoor space located in southern Iran during a dust storm. At first, 44 healthy and non-smoking workers were selected. Then PM10 and Blood samples were collected before and after the dust storm. Finally, HMs associated with PM10 measured by ICP-MS and its effect on the CB, including fibrinogen, CRP, TNF-α, and BP were estimated by ANOVA, Pearson correlation, and Odd Ratio (OR) in SPSS23. Based on the results, the concentration of PM10 and extracted HM such as Cr, As, and Cd was higher than the WHO/EPA standards in dust storms they increased the CB and BP remarkably. Moreover, the level of fibrinogen, blood pressure (BP) and TNF-α in dust storms were higher than in normal conditions (p < 0.05, OR > 3). In addition, As and Cd decreased fibrinogen concentration and systolic BP, respectively. Whereas, TNF-α was associated with concentration of Pb (R = − 0.85) on normal days. Consequently, the HM on PM10 such as As, interferes with the level of investigated CB. These results considered a potential risk for the residents in the southern regions of Iran.
Machine learning algorithms reveal potential miRNAs biomarkers in gastric cancer
Authors: Azari Hanieh , Nazari Elham , Mohit Reza , Asadnia Alireza , Maftooh Mina , Nassiri Mohammadreza , Hassanian Seyed Mahdi , Ghayour-Mobarhan Majid , Shahidsales Soodabeh , Khazaei Majid , Ferns Gordon A. , Avan Amir
Published: April 2023

Gastric cancer is the high mortality rate cancers globally, and the current survival rate is 30% even with the use of combination therapies. Recently, mounting evidence indicates the potential role of miRNAs in the diagnosis and assessing the prognosis of cancers. In the state-of-art research in cancer, machine-learning (ML) has gained increasing attention to find clinically useful biomarkers. The present study aimed to identify potential diagnostic and prognostic miRNAs in GC with the application of ML. Using the TCGA database and ML algorithms such as Support Vector Machine (SVM), Random Forest, k-NN, etc., a panel of 29 was obtained. Among the ML algorithms, SVM was chosen (AUC:88.5%, Accuracy:93% in GC). To find common molecular mechanisms of the miRNAs, their common gene targets were predicted using online databases such as miRWalk, miRDB, and Targetscan. Functional and enrichment analyzes were performed using Gene Ontology (GO) and Kyoto Database of Genes and Genomes (KEGG), as well as identification of protein–protein interactions (PPI) using the STRING database. Pathway analysis of the target genes revealed the involvement of several cancer-related pathways including miRNA mediated inhibition of translation, regulation of gene expression by genetic imprinting, and the Wnt signaling pathway. Survival and ROC curve analysis showed that the expression levels of hsa-miR-21, hsa-miR-133a, hsa-miR-146b, and hsa-miR-29c were associated with higher mortality and potentially earlier detection of GC patients. A panel of dysregulated miRNAs that may serve as reliable biomarkers for gastric cancer were identified using machine learning, which represents a powerful tool in biomarker identification.
Synesthesia has specific cognitive processing during Go/No-go paradigms
Authors: Aoki Yu , Shibasaki Manabu , Nakata Hiroki
Published: April 2023

Grapheme-color synesthesia is a consistent and automatic perception of non-physical color when presented with a grapheme. Many previous studies focused on the synesthetic visual system, but other cognitive functions in grapheme-color synesthetes have remained unclear. Therefore, the objective of the present study was to investigate the characteristics of cognitive processing for motor execution and inhibition during Go/No-go paradigms in grapheme-color synesthesia using event-related potentials (ERPs). Six grapheme-color synesthetes and 24 non-synesthetes performed visual, auditory, and somatosensory Go/No-go paradigms. Omission errors were higher in grapheme-color synesthetes than non-synesthetes. Group-trial interactions (i.e., synesthetes–non-synesthetes × Go–No-go) were observed for the latency of the visual N2 component and amplitude of the somatosensory N2 component. Latencies of auditory and somatosensory P3 components were shorter in grapheme-color synesthetes than non-synesthetes. These findings suggest that grapheme-color synesthetes have specific cognitive processing in motor execution and inhibition as well as synesthetic color perception. Our data advance understanding of cognitive processing in grapheme-color synesthesia.
Application of wrapper based hybrid system for classification of risk tolerance in the Indian mining industry
Authors: Kumar Deepak , Bhattacharjee Ram Madhab
Published: April 2023

The degree to which an individual is willing to take risks i.e., risk tolerance is often cited as a significant causal element in the majority of workplace accidents. It is essential to determine the risk tolerance level of miners and utilise their risk profiles to design improved training modules, safety, recruitment, and deployment policies. This paper aims to identify the most critical factors (or features) influencing miners’ risk tolerance in the Indian coal industry and develop a robust prediction model to learn their risk tolerance levels. To do end, we first conducted a questionnaire survey representing the complete feature set (with 36 features) among 360 miners and divided their responses into five classes of risk tolerance. Next, we propose a wrapper based hybrid system that combines particle swarm optimization (PSO) and random forest (RF) to train a multi-class classifier with a subset of features. In general, the proposed system selects the best feature subset by iteratively generating different feature combinations using the PSO and training an RF classifier model to assess the effectiveness of the generated feature subsets for the F1-score. At last, we compared the PSO-RF with four traditional classification methods to evaluate its effectiveness in terms of precision, recall, F1-score, accuracy, goodness-of-fit, and area under the curve.
Contamination and mortality of leaf-cutting ant workers by the quinone inside inhibitor fungicide after social interactions
Authors: Scudillio Tamires , Camargo Roberto da Silva , Mota Filho Tarcísio Marcos Macedo , de Matos Carlos Alberto Oliveira , Zanuncio José Cola , Sabattini Julian Alberto , Forti Luiz Carlos
Published: April 2023

Leaf-cutting ants of the generaAttaandAcromyrmex(Hymenoptera: Formicidae) are the most important pests in forest and agricultural plantations and livestock. Toxic baits are the main method to manage these insects. The objective was to determine whether the behavior of allogrooming, touch, and self-grooming amongAtta sexdens rubropilosaForel, 1908 (Hymenoptera: Formicidae) workers disperse the fungicide quinone inside inhibitor and whether this product is toxic to them. This fungicide was applied, topically, in groups of workers and the social interactions between them and their mortality with and without the fungicide were evaluated. The interactions and the quinone inside inhibitor fungicide contamination increased with the number of leaf-cutting ant workers per group. Excessive touches, with subsequent allogrooming, and self-grooming among the ant workers dispersed the quinone inside the inhibitor fungicide causing 100% mortality and indicating its toxicity to this insect. The hypothesis that social interactions contaminated ant colony mates and the toxicity of the fungicide quinone inside inhibitor to workers of the leaf-cutting antA. sexdens rubropilosawas proven.