bluuebunny/crossref_metadata_2025 huggingface dataset:
Subversion of a family of antimicrobial proteins by Salmonella enterica
Authors: Gerlach Roman G. , Wittmann Irene , Heinrich Lena , Pinkenburg Olaf , Meyer Torben , Elpers Laura , Schmidt Christiane , Hensel Michael , Schnare Markus
Published: March 2024

Salmonella enterica is a food-borne pathogen able to cause a wide spectrum of diseases ranging from mild gastroenteritis to systemic infections. During almost all stages of the infection process Salmonella is likely to be exposed to a wide variety of host-derived antimicrobial peptides (AMPs). AMPs are important components of the innate immune response which integrate within the bacterial membrane, thus forming pores which lead ultimately to bacterial killing. In contrast to other AMPs Bactericidal/Permeability-increasing Protein (BPI) displayed only weak bacteriostatic or bactericidal effects towards Salmonella enterica sv. Typhimurium (STM) cultures. Surprisingly, we found that sub-antimicrobial concentrations of BPI fold-containing (BPIF) superfamily members mediated adhesion of STM depending on pre-formed type 1 fimbriae. BPIF proteins directly bind to type 1 fimbriae through mannose-containing oligosaccharide modifications. Fimbriae decorated with BPIF proteins exhibit extended binding specificity, allowing for bacterial adhesion on a greater variety of abiotic and biotic surfaces likely promoting host colonization. Further, fimbriae significantly contributed to the resistance against BPI, probably through sequestration of the AMP before membrane interaction. In conclusion, functional subversion of innate immune proteins of the BPIF family through binding to fimbriae promotes Salmonella virulence by survival of host defense and promotion of host colonization.
Applying artificial intelligence to accelerate and de-risk antibody discovery
Authors: Musnier Astrid , Dumet Christophe , Mitra Saheli , Verdier Adrien , Keskes Raouf , Chassine Augustin , Jullian Yann , Cortes Mélanie , Corde Yannick , Omahdi Zakaria , Puard Vincent , Bourquard Thomas , Poupon Anne
Published: March 2024

As in all sectors of science and industry, artificial intelligence (AI) is meant to have a high impact in the discovery of antibodies in the coming years. Antibody discovery was traditionally conducted through a succession of experimental steps: animal immunization, screening of relevant clones, in vitro testing, affinity maturation, in vivo testing in animal models, then different steps of humanization and maturation generating the candidate that will be tested in clinical trials. This scheme suffers from different flaws, rendering the whole process very risky, with an attrition rate over 95%. The rise of in silico methods, among which AI, has been gradually proven to reliably guide different experimental steps with more robust processes. They are now capable of covering the whole discovery process. Amongst the players in this new field, the company MAbSilico proposes an in silico pipeline allowing to design antibody sequences in a few days, already humanized and optimized for affinity and developability, considerably de-risking and accelerating the discovery process.
Digital loneliness—changes of social recognition through AI companions
Authors: Jacobs Kerrin Artemis
Published: March 2024

Inherent to the experience of loneliness is a significant change of meaningful relatedness that (usually negatively) affects a person's relationship to self and others. This paper goes beyond a purely subjective-phenomenological description of individual suffering by emphasizing loneliness as a symptomatic expression of distortions of social recognition relations. Where there is loneliness, a recognition relation has changed. Most societies face an increase in loneliness among all groups of their population, and this sheds light on the reproduction conditions of social integration and inclusion. These functions are essential lifeworldly components of social cohesion and wellbeing. This study asks whether “social” AI promotes these societal success goals of social integration of lonely people. The increasing tendency to regard AI Companions (AICs) as reproducers of adequate recognition is critically discussed with this review. My skepticism requires further justification, especially as a large portion of sociopolitical prevention efforts aim to fight an increase of loneliness primarily with digital strategies. I will argue thatAICs rather reproduce than sustainably reduce the pathodynamics of loneliness: loneliness gets simply “digitized.”
Revolutionizing language learning: Unleashing the power of the engage model to supercharge writing skill in cognitively more and less active EFL learners
Authors: Nikbakht Ahmadreza , Neysani Masoud , Amirjalili Forough
Published: March 2024

The ever-growing need for fluency in written English around the world, because of the role of English as the world’s international language, has given priority to finding more effective ways to its teaching. The present study aimed to investigate the effect of using the ENGAGE Model on writing performance of EFL learners through a mixed-method study. The participants of the study were 60 advanced level female EFL learners with the age range of 20 to 30 in one of the private language institutes in Isfahan, Iran. The participants were non-randomly selected from a large pool of advanced female students. The selected participants were assigned to the two groups of the ENGAGE Model (the experimental group) and TBLT (the control group), with 30 students in each. The participants were also specified in terms of their cognitive ability as cognitively more or less active based on their answers to a validated cognitive profile questionnaire. In the quantitative phase of the study, the participants went through the processes of pre-testing, intervention, and post-testing and the data collected were fed into the SPSS software version 26. The results revealed that the ENGAGE Model had a statistically significant effect on the writing development of cognitively more and less active EFL learners. The qualitative findings of the study proved that the cognitively more active learners enjoyed the ENGAGE Model class more than the cognitively less active ones. Likewise, the cognitively more active learners could benefit from the class more than their counterparts in the cognitively less active camp and assessed themselves more positively in terms of L2 writing. The finding of the study suggested that EFL teachers and stakeholders should increase interaction and higher-order thinking, and make connections to learners’ previous learning.
Assessment of hepatic fibrosis with non-invasive indices in subjects with diabetes before and after liver transplantation
Authors: Grancini Valeria , Cogliati Irene , Alicandro Gianfranco , Gaglio Alessia , Gatti Stefano , Donato Maria Francesca , Orsi Emanuela , Resi Veronica
Published: March 2024

Introduction

One of the most common complications of cirrhosis is diabetes, which prevalence is strictly related to severity of hepatopathy. Actually, there are no data on the persistence of post-transplant glucose abnormalities and on a potential impact of diabetes on development of fibrosis in the transplanted liver. To this aim, we evaluated liver fibrosis in cirrhotic subjects before and after being transplanted.

Methods

The study included 111 individuals who had liver transplantation. The assessment was performed before and two years after surgery to investigate a potential impact of the persistence of diabetes on developing de novo fibrosis in the transplanted liver. The degree of fibrosis was assessed using the Fibrosis Index Based on 4 Factors (FIB-4) and the Aspartate to Platelet Ratio Index (APRI).

Results

At pre-transplant evaluation, 63 out of 111 (56.8%) subjects were diabetic. Diabetic subjects had higher FIB-4 (Geometric mean, 95% confidence interval: 9.74, 8.32-11.41 vs 5.93, 4.71-7.46, P<0.001) and APRI (2.04, 1.69-2.47 vs 1.18, 0.90-1.55, P<0.001) compared to non-diabetic subjects. Two years after transplantation, 39 out of 111 (35.1%) subjects remained with diabetes and continued to show significantly higher FIB-4 (3.14, 2.57-3.82 vs 1.87, 1.55-2.27, P<0.001) and APRI (0.52, 0.39-0.69 vs 0.26, 0.21-0.32, P<0.001) compared to subjects without diabetes.

Discussion

Thus, persistence of diabetes after surgery is a possible risk factor for an evolution to fibrosis in the transplanted liver, potentially leading to worsened long-term outcomes in this population.
A potential of iron slag-based soil amendment as a suppressor of greenhouse gas (CH4 and N2O) emissions in rice paddy
Authors: Galgo Snowie Jane C. , Canatoy Ronley C. , Lim Ji Yeon , Park Hyon Chol , Kim Pil Joo
Published: March 2024

Iron slag-based silicate fertilizer (SF) has been utilized as a soil amendment in rice paddy fields for over 50 years. SF, which contains electron acceptors such as oxidized iron (Fe3+) compounds, is known to reduce methane (CH4) emissions, which have a global warming potential (GWP) of 23, higher than that of carbon dioxide (CO2). However, the dynamics of nitrous oxide (N2O), which has a GWP of 265, were questionable. Since the reduced Fe (Fe2+) can react as an electron donor, SF application might suppress N2O emissions by progressing N2O into nitrogen gas (N2) during the denitrification process. To verify the influence of SF application on two major greenhouse gas (GHG) dynamics during rice cultivation, three different kinds of SF were prepared by mixing iron rust (>99%, Fe2O3) as an electron acceptor with different ratios (0, 2.5, and 5%) and applied at the recommended level (1.5 Mg ha−1) for rice cultivation. SF application was effective in decreasing CH4 emissions in the earlier rice cropping season, and seasonal CH4 flux was more highly decreased with increasing the mixing ratio of iron rust from an average of 19% to 38%. Different from CH4 emissions, approximately 70% of seasonal N2O flux was released after drainage for rice harvesting. However, SF incorporation was very effective in decreasing N2O emissions by approximately 40% over the control. Reduced Fe2+ can be simultaneously oxidized into Fe3+ by releasing free electrons. The increased electron availability might develop more denitrification processes into N2 gas rather than NO and N2O and then decrease N2O emissions in the late rice cultivation season. We could find evidence of a more suppressed N2O flux by applying the electron acceptor-added SFs (SF2.5 and SF5.0) to a 49%–56% decrease over the control. The SF application was effective in increasing rice productivity, which showed a negative-quadratic response to the available silicate (SiO2) concentration in the soil at the harvesting stage. Grain yield was maximized at approximately 183 mg kg−1 of the available SiO2 concentration in the Korean rice paddy, with a 16% increase over no-SF application. Consequently, SF has an attractive potential as a soil amendment in rice paddy to decrease GHG emission impacts and increase rice productivity.
Flexible gold standards for transcription factor regulatory interactions in Escherichia coli K-12: architecture of evidence types
Authors: Lara Paloma , Gama-Castro Socorro , Salgado Heladia , Rioualen Claire , Tierrafría Víctor H. , Muñiz-Rascado Luis J. , Bonavides-Martínez César , Collado-Vides Julio
Published: March 2024

Post-genomic implementations have expanded the experimental strategies to identify elements involved in the regulation of transcription initiation. Here, we present for the first time a detailed analysis of the sources of knowledge supporting the collection of transcriptional regulatory interactions (RIs) of Escherichia coli K-12. An RI groups the transcription factor, its effect (positive or negative) and the regulated target, a promoter, a gene or transcription unit. We improved the evidence codes so that specific methods are incorporated and classified into independent groups. On this basis we updated the computation of confidence levels, weak, strong, or confirmed, for the collection of RIs. These updates enabled us to map the RI set to the current collection of HT TF-binding datasets from ChIP-seq, ChIP-exo, gSELEX and DAP-seq in RegulonDB, enriching in this way the evidence of close to one-quarter (1329) of RIs from the current total 5446 RIs. Based on the new computational capabilities of our improved annotation of evidence sources, we can now analyze the internal architecture of evidence, their categories (experimental, classical, HT, computational), and confidence levels. This is how we know that the joint contribution of HT and computational methods increase the overall fraction of reliable RIs (the sum of confirmed and strong evidence) from 49% to 71%. Thus, the current collection has 3912 reliable RIs, with 2718 or 70% of them with classical evidence which can be used to benchmark novel HT methods. Users can selectively exclude the method they want to benchmark, or keep for instance only the confirmed interactions. The recovery of regulatory sites in RegulonDB by the different HT methods ranges between 33% by ChIP-exo to 76% by ChIP-seq although as discussed, many potential confounding factors limit their interpretation. The collection of improvements reported here provides a solid foundation to incorporate new methods and data, and to further integrate the diverse sources of knowledge of the different components of the transcriptional regulatory network. There is no other genomic database that offers this comprehensive high-quality architecture of knowledge supporting a corpus of transcriptional regulatory interactions.
Impact of melphalan day -1 vs day -2 on outcomes after autologous stem cell transplant for multiple myeloma
Authors: Merino Aimee , Shanley Ryan , Rashid Faridullah , Langer Jenna , Dolan Michelle , Tu Sarah , Jurdi Najla El , Rogosheske John , Hanna Kirollos , DeFor Todd , Janakiram Murali , Weisdorf Daniel
Published: March 2024

Background

Melphalan is the most common conditioning regimen used prior to autologous stem cell transplant (ASCT); however, there are varying data on optimal melphalan timing prior to transplant for best safety and efficacy. Historically, ASCT conditioning consisted of melphalan 200 mg/m2 on day 2 (D-2) (48 h prior to ASCT), but many institutions have since adopted a melphalan protocol with administration on day 1 (D-1) (24 h prior to SCT) or split dosing over the 2 days. The optimal timing of melphalan has yet to be determined.

Methods

In this single-center retrospective study, we analyzed transplant outcomes for patients between March 2011 and September 2020 admitted for high-dose, single-agent melphalan 200 mg/m2 on D-1 vs. D-2. The primary outcomes were time to neutrophil and platelet engraftment. Secondary outcomes include incidence of hospital readmission within 30 days, 2-year progression-free survival, and 2-year overall survival.

Results

A total of 366 patients were studied (D-2 n = 269 and D-1 n = 97). The incidence of high-risk cytogenetics was similar between the two groups (37% vs. 40%). Median days to absolute neutrophil count engraftment was similar at 11 days in the D-2 and D-1 cohort (n = 269, range 0–14, IQR 11–11 vs. n = 97, range 0–14, IQR 11–12). Median days to platelet engraftment >20,000/mcL was 18 days for D-2 melphalan (range: 0–28, IQR 17–20) versus 19 days for D-1 melphalan (range: 0–32, IQR 17–21). Overall survival at 2 years post-transplant was similar in both cohorts (94%; p = 0.76), and PFS was 70% in D-2 compared with 78% in D-1 (p = 0.15). In a multivariable model including age and performance status, hospital readmission within 30 days of transplant was higher in the D-1 cohort (odds ratio 1.9; p = 0.01).

Conclusion

This study demonstrates similar neutrophil and platelet engraftment in D-1 and D-2 melphalan cohorts with similar 2-year PFS and OS. Either D-2 or D-1 melphalan dosing schedule is safe and effective.
The endogenous Mtv8 locus and the immunoglobulin repertoire
Authors: Beilinson Helen A. , Erickson Steven A. , Golovkina Tatyana
Published: March 2024

The vast diversity of mammalian adaptive antigen receptors allows for robust and efficient immune responses against a wide number of pathogens. The antigen receptor repertoire is built during the recombination of B and T cell receptor (BCR, TCR) loci and hypermutation of BCR loci. V(D)J recombination rearranges these antigen receptor loci, which are organized as an array of separate V, (D), and J gene segments. Transcription activation at the recombining locus leads to changes in the local three-dimensional architecture, which subsequently contributes to which gene segments are utilized for recombination. The endogenous retrovirus (ERV) mouse mammary tumor provirus 8 (Mtv8) resides on mouse chromosome 6 interposed within the large array of light chain kappa V gene segments. As ERVs contribute to changes in genomic architecture by driving high levels of transcription of neighboring genes, it was suggested that Mtv8 could influence the BCR repertoire. We generated Mtv8-deficient mice to determine if the ERV influences V(D)J recombination to test this possibility. We find that Mtv8 does not influence the BCR repertoire.
Rare earth elements distribution in the Gulf of Cádiz (SW Spain): geogenic vs. anthropogenic influence
Authors: Cobelo-García Antonio , Bernárdez Patricia , Mendoza-Segura Clara , González-Ortegón Enrique , Sánchez-Quiles David , Sánchez-Leal Ricardo , Tovar-Sánchez Antonio
Published: March 2024

There is a current interest in the study of the geochemical behavior of the rare earth elements (REE) in aquatic systems since their identification as potential microcontaminants in natural waters. In this context, here we report the concentrations and patterns of dissolved REE (dREE) in the waters of the Gulf of Cádiz (GoC) and its major estuaries (Guadiana, Tinto-Odiel and Guadalquivir). Contamination in this area by metals has been extensively reported, linked to acid-mine inputs resulting from the mineralization of the Iberian Pyrite Belt, discharged mainly to the Tinto-Odiel river. However, the impact of these inputs on the concentrations and distribution of the dREE in the coastal waters of the GoC has not been addressed yet. Accordingly, elevated concentrations of dREE were obtained in the Tinto-Odiel estuary compared to the Guadiana and Guadalquivir, reflecting the impact of acid-mine drainage on this system. Only a significant fraction of anthropogenic gadolium (Gd) was observed in the Guadalquivir estuary, as a result of the input from major urban areas, where anthropogenic Gd fractions higher than 70% over the total dissolved pool were obtained for the freshwater end-member. Regarding the surface waters of the GoC, typical NE Atlantic distribution patterns of dREEs were obtained, but modified by two different end-members within this region. Accordingly, and despite the low water discharge of the Tinto-Odiel river, its elevated concentrations lead to an increase in the concentrations of dREEs in the nearshore waters of the GoC and producing a distinctive signature as observed for the MREE anomalies and HREE/LREE ratios. At the easternmost stations, close to the Strait of Gibraltar, these signals are also impacted by the inflow of Mediterranean waters and also by the discharge of the Guadalquivir river.
Postoperative outcomes after total sevoflurane inhalation sedation using a disposable delivery system (Sedaconda-ACD) in cardiac surgery
Authors: Labaste François , Cauquil Paul , Lestarquit Magda , Sanchez-Verlaan Pascale , Aljuayli Abdulrahman , Marcheix Bertrand , Geeraerts Thomas , Ferre Fabrice , Vardon-Bounes Fanny , Minville Vincent
Published: March 2024

Introduction

The COVID-19 pandemic prompted our team to develop new solutions for performing cardiac surgery without intravenous anesthetics due to a shortage of these drugs. We utilized an anesthetic conserving device (Sedaconda-ACD) to administer total inhaled anesthesia because specific vaporizers were unavailable for administering inhaled agents during cardiopulmonary bypass (CPB) in our center. We documented our experience and postoperative cardiovascular outcomes. The primary outcome was the peak level of troponin, with secondary outcomes encompassing other cardiovascular complications.

Material and methods

A single-center retrospective study was conducted. We performed a multivariate analysis with a propensity score. This investigation took place at a large university referral center.

Participants

Adult patients (age ≥ 18) who underwent elective cardiac surgery with CPB between June 2020 to March 2021.

Intervention

During the inclusion period, two anesthesia protocols for the maintenance of anesthesia coexisted—total inhaled anesthesia with Sedaconda-ACD and our classic protocol with intravenous drugs during and after CPB.

Primary endpoint

Troponin peak level recorded after surgery (highest level recorded within 48 h following the surgery).

Results

Out of the 654 included patients, 454 were analyzed after matching (intravenous group = 297 and inhaled group = 157). No significant difference was found between the groups in postoperative troponin peak levels (723 ng/l vs. 993 ng/l–p = 0.2). Total inhaled anesthesia was associated with a decreased requirement for inotropic medications (OR = 0.53, 95% CI 0.29–0.99, p = 0.04).

Conclusion

In our cohort, the Sedaconda-ACD device enabled us to achieve anesthesia without intravenous agents, and we did not observe any increase in postoperative complications. Total inhaled anesthesia with sevoflurane was not associated with a lower incidence of myocardial injury assessed by the postoperative troponin peak level. However, in our cohort, the use of inotropic drugs was lower.
Alterations in urinary ceramides, sphingoid bases, and their phosphates among patients with kidney disease
Authors: Morita Yoshifumi , Sakai Eri , Isago Hideaki , Ono Yoshikazu , Yatomi Yutaka , Kurano Makoto
Published: March 2024

Background

To avoid an invasive renal biopsy, noninvasive laboratory testing for the differential diagnosis of kidney diseases is a desirable goal. As sphingolipids are demonstrated to be involved in the pathogenesis of various kidney diseases, we investigated the possible usefulness of the simultaneous measurement of urinary sphingolipids for differentiating kidney diseases.

Materials and methods

Residual urine specimens were collected from patients who had been clinically diagnosed with chronic glomerulonephritis (CGN), diabetic mellitus (DM), systemic lupus erythematosus (SLE), and arterial hypertension (AH). The urinary sphingolipids—CERs C16:0, C18:0, C18:1, C20:0, C22:0, and C24:0; sphingosine [Sph]; dihydrosphingosine; sphingosine 1-phosphate [S1P]; and dihydroS1P [dhS1P]—were measured by liquid chromatography–tandem mass spectrometry. Based on the results, machine learning models were constructed to differentiate the various kidney diseases.

Results

The urinary S1P was higher in patients with DM than in other participants (P < 0.05), whereas dhS1P was lower in the CGN and AH groups compared with control participants (P < 0.05). Sph and dhSph were higher in patients with CGN, AH, and SLE than in those with control participants (P < 0.05). The urinary CERs were significantly higher in patients with CGN, AH, and SLE than in those with control participants (P < 0.05). As a results of constructing a machine learning model discriminating kidney diseases, the resulting diagnostic accuracy and precision were improved from 94.03% and 66.96% to 96.10% and 78.26% respectively, when the urinary CERs, Sph, dhSph, S1P, dhS1P, and their ratios were added to the models.

Conclusion

The urinary CERs, sphingoid bases, and their phosphates show alterations among kidney diseases, suggesting their potential involvement in the development of kidney injury.
Preoperative differentiation of gastric schwannomas and gastrointestinal stromal tumors based on computed tomography: a retrospective multicenter observational study
Authors: Zhao Luping , Cao Guanjie , Shi Zhitao , Xu Jingjing , Yu Hao , Weng Zecan , Mao Sen , Chen Yueqin
Published: March 2024

Introduction

Gastric schwannoma is a rare benign tumor accounting for only 1–2% of alimentary tract mesenchymal tumors. Owing to their low incidence rate, most cases are misdiagnosed as gastrointestinal stromal tumors (GISTs), especially tumors with a diameter of less than 5 cm. Therefore, this study aimed to develop and validate a diagnostic nomogram based on computed tomography (CT) imaging features for the preoperative prediction of gastric schwannomas and GISTs (diameters = 2–5 cm).

Methods

Gastric schwannomas in 47 patients and GISTs in 230 patients were confirmed by surgical pathology. Thirty-four patients with gastric schwannomas and 167 with GISTs admitted between June 2009 and August 2022 at Hospital 1 were retrospectively analyzed as the test and training sets, respectively. Seventy-six patients (13 with gastric schwannomas and 63 with GISTs) were included in the external validation set (June 2017 to September 2022 at Hospital 2). The independent factors for differentiating gastric schwannomas from GISTs were obtained by multivariate logistic regression analysis, and a corresponding nomogram model was established. The accuracy of the nomogram was evaluated using receiver operating characteristic and calibration curves.

Results

Logistic regression analysis showed that the growth pattern (odds ratio [OR] 3.626; 95% confidence interval [CI] 1.105–11.900), absence of necrosis (OR 4.752; 95% CI 1.464–15.424), presence of tumor-associated lymph nodes (OR 23.978; 95% CI 6.499–88.466), the difference between CT values during the portal and arterial phases (OR 1.117; 95% CI 1.042–1.198), and the difference between CT values during the delayed and portal phases (OR 1.159; 95% CI 1.080–1.245) were independent factors in differentiating gastric schwannoma from GIST. The resulting individualized prediction nomogram showed good discrimination in the training (area under the curve [AUC], 0.937; 95% CI, 0.900–0.973) and validation (AUC, 0.921; 95% CI, 0.830–1.000) datasets. The calibration curve showed that the probability of gastric schwannomas predicted using the nomogram agreed well with the actual value.

Conclusion

The proposed nomogram model based on CT imaging features can be used to differentiate gastric schwannoma from GIST before surgery.
Effect of Sn nanoparticles on the optical properties of PEDOT:PSS thin films
Authors: Aly Kamal A. , Ismail Yasser A. M. , Salman S. Alsulami Ziad , Abolibda Tariq Z. , Almohammedi Abdullah , Gomha Sobhi M. , Ahmed Hoda A. , Ibrahim Mohamed S.
Published: March 2024

Introduction: In this study, we focus on enhancing the optical properties of PEDOT:PSS thin films by incorporating pure Sn nanoparticles (NPs) synthesized using the ultrasonic ablation technique. The objective is to investigate the impact of Sn concentration on the optical characteristics of the films, with a specific emphasis on applications in organic solar cells.

Methods: We systematically varied the concentrations of Sn in PEDOT:PSS thin films and characterized their optical properties. The index of refraction (n) and extinction coefficient (k) were precisely determined by analyzing the transmission and reflection spectra of the films. Additionally, Sellmeier’s dispersal model was employed to elucidate the obtained results of n, and dispersive factors were calculated and interpreted.

Results: The incorporation of Sn nanoparticles led to improvements in the energy bandgap (Eg) values of PEDOT:PSS films. Notably, as the concentration of Sn increased, the n values decreased, indicating enhanced suitability for organic solar cell applications. The study also unveiled a decrease in the dielectric constant of PEDOT:PSS/Sn films with increasing Sn content, resulting in improved transmittance velocity and enhanced efficacy of microelectronic devices. This, in turn, promotes the development of large-frequency and large-velocity stretchy circuit boards.

Discussion: The comprehensive assessment of optical and dielectric parameters, including complex dielectric constant, complex optical conductance, and nonlinear optical constants, provides valuable insights into the potential applications of PEDOT:PSS/Sn films. The larger nonlinear optical constants observed in the present films suggest their suitability for diverse applications such as all-optical switching, limiting, phase modulation, and frequency conversion. Overall, our findings highlight the promising potential of Sn-incorporated PEDOT:PSS thin films in advancing the field of optoelectronics and microelectronics.
Image security using steganography and cryptography with sweeping computational ghost imaging
Authors: Rajabi-Ghaleh Sajjad , Olyaeefar Babak , Kheradmand Reza , Ahmadi-Kandjani Sohrab
Published: March 2024

A sweeping computational ghost imaging (SCGI)-based encryption system is intended for increased data security and speedier data transport. SCGI is combined with steganography and cryptography processes in this system. SCGI requires fewer shots, resulting in faster image capture, transmission, encryption, and decryption. This strategy also results in smaller, more compact data packages and higher bitrates. Least significant bit (LSB) uses steganography to conceal the hidden picture. In the case of Rivest–Shamir–Adleman (RSA) encryption, public and private keys are generated via a one-way function based on bucket values. Encryption is performed on two levels, with an asymmetric approach divided into three sub-items that significantly increase encryption. Surprisingly, the method uses fewer pictures for image reconstruction, resulting in faster image reconstruction. SCGI promises applications in an extensive number of data encryption sectors since this technology leads to smaller data packages and higher bitrates. The presented approach is examined using the number of pixel change rate (NPCR), normalized root mean square (NRMS), peak signal-to-noise ratio (PSNR), and correlation coefficient (CC), which indicates constant encryption improvement. We experimentally and situationally demonstrate our findings under eavesdropping, which prove the resistance and robustness of our methods. In optimal settings, this innovation enhances encryption by up to 90% when compared to traditional encryption methods.
The implications of internet-based Chinese language courses on online classes
Authors: Khan Rabnawaz , Zhuang Weiqing
Published: March 2024

Interactionist and social-cultural perspectives on second language acquisition suggest that interactions between teachers and students offer promising avenues for acquiring Chinese as a second language, which the vast majority of international students consider difficult. Computer-mediated communication is far safer than face-to-face encounters during the present pandemic. Three aims are being investigated here. It is important to first analyze the differences between traditional classroom and online learning by different modes, then analyze the various ways teachers use computer-mediated communication, and finally analyze the challenges and opportunities presented by online Chinese as a second language courses using qualitative research methods. Three teachers and 84 students are analyzed statistically in terms of their multimodal interactions, and the quality of their weekly classroom exchanges is assessed through an interpretive analysis of questionnaire data, all in the name of a mixed-methods approach. Particular attention was paid to the challenges of online tutoring for students, the discrepancy between instructor and student understandings, and the use of several teaching strategies with international students. The online classroom environment places unique demands on the quality of student-teacher communication. Different strategies must be used when teaching non-native speakers of Chinese as a second language compared to teaching in a traditional classroom setting.
TVET programme and health-related quality of life among low-income populations during the COVID-19 pandemic in Malaysia
Authors: Yasin Ruhizan Mohammad , Tan Maw Pin , Said Mas Ayu , Rasul Mohd Sattar , Thangiah Nithiah , Rizal Hussein , Magli Amirah Shazana , Ithnin Muslimah , Majid Hazreen Abdul , Ismail Rozmi , Su Tin Tin
Published: March 2024

Introduction

Education improves the economy and quality of life. The availability of skilled education in Malaysia is not restricted to the younger generation but is available to people of all ages, including those with low incomes.

Methods

This study used the EuroQol 5-Dimension 5-Level (EQ- 5D-5L) tool during the COVID-19 pandemic to examine relationships between socio-demographics, knowledge, and attitudes towards education and outcomes of health-related quality of life (HRQOL). Between September and October 2020 and January and February 2021, a cross-sectional study using a multi-stage sampling technique was carried out.

Results

A total of 1,997 adults participated, with a mean age of 45.17 (SD 14.113). In total, 74.9% had good knowledge, while 59.8% had a positive attitude towards skill education. In univariate analyses, the EQ-5D-5L score was related to age, income, education level, marital status, employment status, financial strain level, and knowledge and attitude towards skilled education. Generalised linear model analyses demonstrated that lower EQ-5D-5L scores were associated with older age, financial constraints, and a negative attitude towards skills education. However, additional adjustments for knowledge and attitude towards skills education show only an increase in age and financial strain was significant.

Conclusion

The findings suggest that appropriate strategies be implemented to increase low-income populations’ knowledge and attitude towards skill education. Improving education may improve the quality of life for this vulnerable group. Additionally, a qualitative study can be conducted to determine the barriers to low-income households participating in skilled education to fill in the knowledge gap.
Associations of objectively measured physical activity and sleep in preschoolers aged 3 to 6 years
Authors: Miller Laura , Dockrill Mya , Corkum Penny V. , Kirk Sara F. L. , Stone Michelle
Published: March 2024

Research has demonstrated various negative effects of poor sleep on overall health in children. Engaging in physical activity during the day is often recommended to help children sleep better. Limited research has examined this recommendation for preschool children, although physical activity is generally supported as a healthy sleep practice. When measuring physical activity and sleep, objective measures (e.g., accelerometers) are recommended as opposed to subjective measures (e.g., parental reports). The purpose of the current study was to examine the relationship between objectively measured sleep (key variables included sleep efficiency, sleep onset latency, and sleep duration) and physical activity (operationalized as mean daily total physical activity) among preschool-aged children in Nova Scotia, Canada. Children (n = 29) wore a wrist accelerometer to objectively measure sleep and wore an accelerometer on their waist to measure physical activity for nine consecutive days. Overall, linear regression analyses demonstrate physical activity as a predictor of sleep efficiency but not total sleep time or sleep onset latency among preschool-aged children. Future research should examine the causal relationships between sleep efficiency and physical activity by conducting interventions to increase physical activity and determining the impact on sleep efficiency.
Transformative experiences in tourism: where, when, with whom, and how does tourists' transformation occur?
Authors: Godovykh Maksim
Published: March 2024

Travel experiences have the potential to induce significant changes in tourists. This perspective paper aims to delineate directions for understanding the where, when, with whom, and why of transformative experiences in tourism, proposing future research directions across various dimensions of this multifaceted topic. The Transformative Experience Diagram is presented as a guide to addressing questions about where, when, with whom, and why travel transformation occurs. These propositions are grounded in a literature review spanning various disciplines, along with authors' quantitative and qualitative studies on the process of travel transformation and discussions with experts from diverse fields. Additional data, gathered from semi-structured interviews with people who previously had transformative travel experiences, inform the discussion on key questions of travel transformation and support the propositions for future research. In addition to conceptualizing the process of tourist transformation and proposing a framework for future research, this perspective paper has the potential to influence tourism management practices by guiding the design of experiences that trigger tourists' transformation, positively impacting personal growth, and enhancing the wellbeing of travelers.
The feeling of being classified: raising empathy and awareness for AI bias through perspective-taking in VR
Authors: Schlagowski Ruben , Volanti Maurizio , Weitz Katharina , Mertes Silvan , Kuch Johanna , André Elisabeth
Published: March 2024

In a world increasingly driven by AI systems, controversial use cases for AI that significantly affect people’s lives become more likely scenarios. Hence, increasing awareness of AI bias that might affect underprivileged groups becomes an increasing challenge. As Virtual Reality has previously been shown to increase empathy through immersive perspective-taking, we conducted a laboratory study in which participants were confronted with a biased Wizard of Oz AI while embodying personas that varied widely in their ability to achieve high financial credit scores due to their age and gender. We found that participants embodying personas in VR felt significantly more empathy toward the characters they embodied and rated the AI as significantly less fair compared to a baseline condition in which they imagined to be these characters. Furthermore, we investigate differences between embodied personas and discuss qualitative results to gain insight into the participant’s mental model creation.
A new technique of autologous bone grafting for open-wedge high tibial osteotomy
Authors: Gong Yongchao , Jin Lin , Wang Yanwei , Liu Boxu , Shen Pengfei , Zhang Zhiang , Yuwen Peizhi , Zhao Kuo , Ma Lijie , Chen Wei , Zhang Yingze
Published: March 2024

Purpose

This study aimed to demonstrate the application of orthotopic bone flap transplantation with a fibula transplantation (OBFT-FT) in open-wedge high tibial osteotomy (OW-HTO) and to assess the effect of OBFT-FT on gap healing.

Patients and methods

From January to July 2020, 18 patients who underwent OW-HTO with OBFT-FT were reviewed for this study. Demographics, postoperative complications, and radiological and clinical outcomes of patients were collected. Finally, the clinical outcomes of patients were analyzed.

Results

A total of 14 patients were included in this study. The average age and body mass index were 59.6 ± 9.2 years and 28.1 ± 4.5 kg/m2, respectively. The average correction angle and gap width were 9.5 ± 1.8° and 10.2 ± 2.7 mm, respectively. The rates of radiological gap healing at sixth week, third month, and sixth month were 42.9%, 85.7%, and 100%, respectively. The mean Lysholm score, International Knee Documentation Committee score, and visual analog scale scores at sixth-month follow-up were significantly better than the preoperative scores (p < 0.001, p < 0.001, p = 0.001, respectively). And, no delayed union or non-union, collapse, loss of correction, or surgical site infection were found.

Conclusions

As a new technique for autologous bone graft, the OBFT-FT could be successfully applied in the treatment of gap healing after OW-HTO, and excellent radiological and clinical outcomes could be seen on patients’ short-term follow-up.
Pedicled flap transfer after chest wall malignant tumor resection and potential risk of postoperative respiratory problems for patients with low FEV1.0%
Authors: Asanuma Kunihiro , Tsujii Masaya , Hagi Tomohito , Nakamura Tomoki , Uchiyama Teruya , Adachi Ryohei , Nakata Kenta , Kataoka Takeshi , Sudo Akihiro
Published: March 2024

Introduction

Musculoskeletal transfer for chest wall tissue defects is a crucial method, and pedicled flaps around the chest wall are preferred in terms of location and simplicity of transfer. These require special care because of complications such as partial necrosis, fistula, wound dehiscence, infection, hematoma and restricted function of the arm or shoulder. However, studies of respiratory function are rare. In the present study, we investigated the complications including respiratory problems after wide resection for malignant chest wall tumors with musculoskeletal pedicle transfer.

Methods

A total of 13 patients (15 operations) who underwent wide resection of primary, recurrent, or metastatic malignant chest wall tumors and musculoskeletal pedicle transfer for coverage of tissue defects were enrolled in the present study. A retrospective review of all patients was performed using data collected from hospital records and follow-up information. The complications of musculoskeletal transfer after chest wall wide resection, including respiratory problems, are evaluated.

Results

Rib or sternal resection was performed in 12 operations, and only soft tissue resection was performed in 3 operations. Latissimus dorsi (LD) pedicle transfer was performed in 13 operations, and pectoralis major (PM) pedicle transfer was performed in 2 operations; basically, wounds were closed primarily. Surgical complications were observed following 5 of the 15 operations (33.3%). Respiratory complications were seen in 7 of the 15 operations (46.7%). Patients with respiratory complications showed significantly lower preoperative FEV1.0% values than those without respiratory complications (p = 0.0196). Skin resection area tended to be higher in the complication group than in the no complication group (p = 0.104).

Discussion

Pedicled myocutaneous flap transfers such as LD, PM, and rectus abdominus can be used following multiple resections. After harvesting LD or PM, the wound can be closed primarily for an 8–10-cm skin defect in patients with normal respiratory function. However, for patients with low FEV1.0%, after primary closure of LD or PM transfer for wide soft tissue defects, attention should be paid to postoperative respiratory complications.
Crystal induced arthropathies—a comparative study of 40 patients with apatite rheumatism, chondrocalcinosis and primary synovial chondromatosis
Authors: Bély Miklós , Apáthy Ágnes
Published: March 2024

Introduction: Apatite rheumatism (AR), chondrocalcinosis (Ch-C), and primary synovial chondromatosis (prSynCh) are regarded as distinct clinical entities. The introduction of the non-staining technique by Bély and Apáthy (2013) opened a new era in the microscopic diagnosis of crystal induced diseases, allowing the analysis of MSU (monosodium urate monohydrate) HA (calcium hydroxyapatite), CPPD (calcium pyrophosphate dihydrate) crystals, cholesterol, crystalline liquid lipid droplets, and other crystals in unstained sections of conventionally proceeded (aqueous formaldehyde fixed, paraffin-embedded) tissue samples. The aim of this study was to describe the characteristic histology of crystal deposits in AR, Ch-C, and prSynCh with traditional stains and histochemical reactions comparing with unstained tissue sections according to Bély and Apáthy (2013).

Patients and methods: Tissue samples of 4 with apatite rheumatism (Milwaukee syndrome), 16 with chondrocalcinosis, and 20 with clinically diagnosed primary synovial chondromatosis were analyzed.

Results and conclusion: Apatite rheumatism, chondrocalcinosis, and primary synovial chondromatosis are related metabolic disorders with HA and CPPD depositions. The authors assume that AR and Ch-C are different stages of the same metabolic disorder, which differ from prSynCh in amorphous mineral production, furthermore in the production of chondroid, osteoid and/or bone. prSynCh is a defective variant of HA and CPPD induced metabolic disorders with reduced mineralization capabilities, where the deficient mineralization is replaced by chondroid and/or bone formation. The non-staining technique of Bély and Apáthy proved to be a much more effective method for the demonstration of crystals in metabolic diseases than conventional stains and histochemical reactions.
Talent Management and Generation Z: A Systematic Literature Review through the Lens of Employer Branding
Authors: Vieira Jorge , Gomes da Costa Carla , Santos Vasco
Published: March 2024

Generation Z is arriving on the labour scene. In the near future, this generation will dominate organisations’ recruitment and selection processes. Theories regarding the attraction and retention of talent were initially developed based on the characteristics of previous generations in the late 20th century. This study aimed to conduct the first comprehensive literature review focused on identifying the attributes of attracting and retaining Generation Z talent. The findings will allow for the identification of the most significant attributes within the examined context. However, certain gaps were uncovered, such as the limited availability of publications explaining the applicability of the conventional theory of attracting and retaining talent to Generation Z natives. Significant lines of research are also suggested for the future.
Physicochemical Characterization of ‘Moroccan Picholine’ Olive (Olea europaea L.) Oil Produced in Southern Morocco Using Multivariate Statistical Analysis
Authors: Gagour Jamila , Hallouch Otmane , Asbbane Abderrahim , Laknifli Abdellatif , Sakar El Hassan , Majourhat Khalid , Gharby Said
Published: March 2024

This study focuses on evaluating the characteristics of olive oil produced in the Taroudant province (southern Morocco), making this the first comprehensive study focusing on olive oil from the ‘Moroccan Picholine’ cultivar. Our objective was to elucidate the distinctive qualities of olive oil from this region, providing valuable insights into its potential contributions to the country’s olive oil sector. For this purpose, several quality criteria (free fatty acids, moisture content, saponification value, and iodine value), oxidation indices (peroxide value, specific UV extinction coefficients, and oxidizability value), and purity indices (fatty acids and sterol composition) were evaluated. Our results reveal minor-to-significant variations (p < 0.05) in the quality and oxidation parameters. Specifically, our results indicate diverse ranges for free fatty acids (0.33–3.62 g/100 g), peroxide values (0.85–4.01 mEq O2/kg oil), K232 (1.68 to 2.73), and K270 (0.09–0.34). Furthermore, consistently high levels of oleic acid (55.8 to 73.1%) and β-sitosterol (94.2 to 97%) were observed in the studied samples. These outcomes were confirmed through the use principal component analysis and hierarchical cluster analysis. Likewise, important correlations were outlined among the studied parameters. Multidimensional analyses not only highlight inherent variations, but also facilitate the classification of the analyzed olive oils into distinct categories. The results suggest that the Taroudant province exhibits favorable conditions for producing high-quality olive oil.