bluuebunny/crossref_metadata_2025 huggingface dataset:
Induction of Tier 2 HIV-Neutralizing IgA Antibodies in Rhesus Macaques Vaccinated with BG505.664 SOSIP
Authors: Smith Justin C. , Arunachalam Prabhu S. , Legere Traci H. , Cavacini Lisa A. , Hunter Eric , Pulendran Bali , Amara Rama R. , Kozlowski Pamela A.
Published: December 2024

Background: A goal of mucosal human immunodeficiency virus type 1 (HIV-1) vaccines is to generate mucosal plasma cells producing polymeric IgA (pIgA)-neutralizing antibodies at sites of viral entry. However, vaccine immunogens capable of eliciting IgA neutralizing antibodies (nAbs) that recognize tier 2 viral isolates have not yet been identified. Methods: To determine if stabilized native-like HIV-1 envelope (Env) trimers could generate IgA nAbs, we purified total IgA and IgG from the banked sera of six rhesus macaques that had been found in a previous study to develop serum nAbs after subcutaneous immunization with BG505.664 SOSIP and 3M-052 adjuvant, which is a TLR7/8 agonist. The neutralization of autologous tier 2 BG505 T332N pseudovirus by the IgA and IgG preparations was measured using the TZM-bl assay. Anti-SOSIP binding antibodies (bAbs) were measured by ELISA. Results: The IgG samples were found to have significantly greater levels of both nAb and bAb. However, after normalizing the nAb titer relative to the concentration of bAb, SOSIP-specific IgA purified from 2/6 animals was found to neutralize just as effectively as SOSIP-specific IgG, and in 3/6 animals, neutralization by the specific IgA was significantly greater. The more potent neutralization by IgA in these three animals was associated with a higher percentage of anti-SOSIP J chain-bound (polymeric) antibody. Conclusions: The parenteral vaccination of nonhuman primates with BG505.664 SOSIP generates HIV-1 tier 2 IgA nAbs in serum, including SOSIP-specific polymeric IgA, which appears to neutralize more efficiently than monomeric IgA or IgG. Mucosal delivery of this SOSIP or other stable Env trimers could generate locally synthesized polymeric IgA nAbs in mucosal tissues and secretions.
Morphological and Molecular Characterization of Tick Species Infesting Cattle in South Africa
Authors: Makwarela Tsireledzo Goodwill , Nyangiwe Nkululeko , Masebe Tracy Madimabi , Djikeng Appolinaire , Nesengani Lucky Tendani , Smith Rae Marvin , Mapholi Ntanganedzeni Olivia
Published: December 2024

Ticks are a significant threat to livestock globally, with certain species displaying distinct host preferences at various developmental stages. Accurate species-level identification is essential for studying tick populations, implementing control strategies, and understanding disease dynamics. This study evaluated ticks infesting cattle across six provinces in South Africa using morphological and molecular methods. Ticks were preserved, examined morphologically using an Olympus Digital Camera Microscope, and identified using the 16S rRNA gene. The study identified four genera, namely Amblyomma, Hyalomma, Ixodes, and Rhipicephalus, comprising 15 ixodid species. Amblyomma hebraeum was the most prevalent species, with an infestation rate of 54.4%. Molecular analysis revealed genetic relationships among tick species, with genetic distances ranging from 0.00 to 0.13, and phylogenetic analysis clustered species into distinct genera with high bootstrap support. Principal Component Analysis highlighted clear genetic relatedness among species. These findings enhance our understanding of tick diversity, morphology, and distribution in South Africa’s cattle populations, emphasizing their economic significance. The study provides critical baseline data for monitoring and developing effective strategies to manage tick-borne diseases, ensuring improved livestock health and productivity.
Gut evacuation rate as a tool for revealing feeding patterns in the invasive round goby (Neogobius melanostomus) under different feeding modes, food types and temperatures
Authors: Szydłowska Natalia Z. , Let Marek , Franta Pavel , Buřič Miloš , Worischka Susanne , Richter Luise , Drozd Bořek
Published: December 2024

The round goby (Neogobius melanostomus) is a well-known invasive fish. Knowledge of its feeding habits and means of food processing is key in understanding its impact on aquatic food webs. The present study assessed the gut evacuation rate of round gobies feeding on three different types of prey occurring naturally in the diet of this species (small native freshwater clams, an invasive amphipod and chironomid larvae), at two different temperatures (14 and 20 °C) and under different food availability scenarios (continuous and non-continuous feeding). Gut evacuation rates varied significantly between the prey availability scenarios and, specifically, round gobies processed prey significantly faster in the continuous feeding mode when food was regularly available than when fed only once. The highest evacuation rates were detected for individuals fed with clams, in which complete gut clearance was observed within 16 h, compared to within 24 h and 36 h for chironomid larvae and amphipods, respectively. Our study shows that round gobies evacuate chironomid and mollusc prey most rapidly, which suggests that potentially the highest predatory pressure will be exerted on these prey types, assuming that all three prey species are locally present. The slower processing and digestion of amphipods may be due to their bulkier shape, which makes them more difficult to swallow. The relatively high evacuation efficiency of the round goby observed in the continuous feeding mode suggests overall increased pressure on food resources, thereby potentially reducing availability for other consumers and accelerating resource depletion, mainly driven by the high local densities of the round goby populations.
Wooden panels against noise pollution in buildings and structures
Authors:   Siberian Federal University, Guzovatova A. D. , Zaitsev T. L. ,   Siberian Federal University, Bondareva V. K. ,   Siberian Federal University, Felsinger D. A. ,   Siberian Federal University, Khramov I. V. ,   Siberian Federal University
Published: December 2024

This paper provides an overview of noise pollution and its effects on human health,
as well as examples of wooden sound insulation panels. Noise causes physiological and
psychological reactions, focusing on both short-term and long-term effects. In addition,
the article discusses the sources of noise pollution, types of sound insulation materials,
as well as strategies to reduce human exposure. Analyzing scientific sources, we can talk
about noise pollution and its potential danger to human health. The paper highlights the need to improve evidence-based means and methods of protection against noise
pollution to protect public health from harmful and negative effects of noise. The purpose
of this work is to review the effects of noise pollution on humans, the types of sound
insulation panels, as well as the proposed method of noise protection.
Light demarcation of hazardous areas of a timber enterprise
Authors:   Safety Technologies, Kondratova N. I.
Published: December 2024

Industrial accidents are a serious threat to workers and organizations. In addition to the
physical and emotional impact on the victims, such incidents have a major impact on the
legal and financial aspects of the organization»s activities. In order to prevent negative
consequences and ensure the safety of personnel, it is necessary to take appropriate
measures and comply with laws and safety regulations at work. The article considers the
light demarcation of hazardous areas as a tool to reduce injuries at timber enterprises.
Prevalence and patterns of testing for anaemia in primary care in England: a cohort study using an electronic health records database
Authors: Smith Margaret , Drakesmith Cynthia Wright , Haynes Sarah , Maynard Suzanne , Shah Akshay , Roy Noemi BA , Lee Joseph Jonathan , Maurer Katja , Stanworth Simon J , Bankhead Clare R
Published: December 2024

Background

Despite epidemiological data on anaemia being available on a global scale, the prevalence of anaemia in the UK is not well described.

Aim

To describe anaemia prevalence and testing patterns for haemoglobin and other blood parameters.

Design and setting

This study was a descriptive population-based cohort study using data drawn from the Clinical Practice Research Datalink Aurum database in 2019.

Method

Demographic data were extracted for each person who was registered at their current practice during 2019, including linked data on Index of Multiple Deprivation. Anaemia prevalence in 2019 was calculated based on World Health Organization-specified age and gender thresholds for haemoglobin. Anaemia was classified based on mean corpuscular volume and ferritin. People with anaemia were followed up for up to 1 year to investigate longitudinal testing patterns for haemoglobin.

Results

The cohort contained 14 million people. Anaemia prevalence in England in 2019 was 4.1% (583 847/14 207 841) (5.1% [363 438/7 121 614] females and 3.1% [220 409/7 086 227] males). Prevalence was higher in people aged >65 years, people of Black and Asian ethnicities, and people living in areas with higher social deprivation. Only half of people with anaemia and a mean corpuscular volume of ≤100 fL had an accompanying ferritin value recorded. About half of people with anaemia had a follow-up haemoglobin test within 1 year, most of which still indicated anaemia.

Conclusion

Anaemia is prevalent in the UK with large disparities between levels of demographic variables. Investigation and follow-up of anaemia is suboptimal in many patients. Health interventions aimed at improving anaemia investigation and treatment are needed, particularly in the most at-risk groups.
Spatially Resolved Multiomics: Data Analysis from Monoomics to Multiomics
Authors: Huan Changxiang , Li Jinze , Li Yingxue , Zhao Shasha , Yang Qi , Zhang Zhiqi , Li Chuanyu , Li Shuli , Guo Zhen , Yao Jia , Zhang Wei , Zhou Lianqun
Published: December 2024

Spatial monoomics has been recognized as a powerful tool for exploring life sciences. Recently, spatial multiomics has advanced considerably, which could contribute to clarifying many biological issues. Spatial monoomics techniques in epigenomics, genomics, transcriptomics, proteomics, and metabolomics can enhance our understanding of biological functions and cellular identities by simultaneously measuring tissue structures and biomolecule levels. Spatial monoomics technology has evolved from monoomics to spatial multiomics. Moreover, the spatial resolution, high-throughput detection capability, capture efficiency, and compatibility with various sample types of omics technology have considerably advanced. Despite the technological advances in this field, data analysis frameworks have stagnated. Current challenges include incomplete spatial monoomics data analysis pipeline, overly complex data analysis tasks, and few established spatial multiomics data analysis strategies. In this review, we systematically summarize recent developments of various spatial monoomics techniques and improvements in related data analysis pipeline. On the basis of the spatial multiomics technology, we propose a data integration strategy with cross-platform, cross-slice, and cross-modality. We summarize the potential applications of spatial monoomics technology, aiming to provide researchers and clinicians with a better understanding of how such applications have advanced. Spatial multiomics technology is expected to substantially impact biology and precision medicine through measurements of cellular tissue structures and the extraction of biomolecular features.
Custom-Made Ce–Mn Bimetallic Nanozyme for the Treatment of Intervertebral Disc Degeneration by Inhibiting Oxidative Stress and Modulating Macrophage M1/M2 Polarization
Authors: Wu Jianwei , Chen Zhenhao , Huang Han , Wang Hongwei , Wang Xianghe , Lu Zian , Xu Haocheng , Ma Xiaosheng , Zeng Feng , Wang Hongli
Published: December 2024

Intervertebral disc degeneration (IDD)-induced lower back pain (LBP) brings heavy burden worldwide. In the degenerated intervertebral disc, there is an increase in the accumulation of reactive oxygen species (ROS) and the infiltration of M1 macrophages, which leads to abnormal local inflammatory microenvironment and exacerbates IDD. In this study, we developed a novel injectable polyethylene glycol (PEG)-capped cerium ion–manganese ion (Ce–Mn) bimetallic nanozyme (CeMn-PEG) with strong ROS scavenging and M2-type macrophage polarizing abilities to efficiently alleviate IDD. In vitro experiments demonstrated that CeMn-PEG effectively scavenged excess ROS in both nucleus pulposus (NP) and RAW264.7 cells. In addition, we found that CeMn-PEG markedly protected NP cells from H
2
O
2
-induced overproduction of inflammatory cytokines, excessive cell apoptosis and autophagy, and imbalance between extracellular matrix (ECM) degradation. Moreover, CeMn-PEG induced macrophages to transition from the M1 phenotype to the M2 phenotype and the increased M2-type macrophages could alleviate H
2
O
2
-induced ECM degradation and cell apoptosis in NP cells. In a puncture-induced mouse IDD model, CeMn-PEG treatment could effectively ameliorate the progression of disc degeneration and mitigate puncture-induced mechanical hyperalgesia. Thus, our study demonstrated the effectiveness of CeMn-PEG as a novel treatment strategy for the treatment of IDD and a range of other inflammatory diseases.
Highly Efficient Delivery of Novel MiR-13896 by Human Umbilical Cord Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Inhibits Gastric Cancer Progression by Targeting ATG2A-Mediated Autophagy
Authors: Wu Peipei , Wang Min , Jin Can , Li Linli , Tang Yuting , Wang Zhangfei , Wang Xianwen , Xu Wenrong , Qian Hui
Published: December 2024

Gastric cancer (GC) is the fourth most common cancer and the second leading cause of cancer-related deaths worldwide. Despite recent advancements, clinical outcomes for GC remain unsatisfactory. Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) have shown promise in inhibiting tumor progression, but their role in GC, specifically human umbilical cord MSC-derived small EVs (hucMSC-sEVs), is not well understood. This study investigates the therapeutic potential of hucMSC-sEVs in GC treatment. We found that hucMSC-sEVs are captured by GC cells, substantially inhibiting their proliferation and inducing apoptosis. MiRNA sequencing revealed that hucMSC-sEVs were enriched with miRNAs having anticancer properties. Among these, miR-13896, a new miRNA, was identified as a potent inhibitor of GC cell proliferation and a promoter of apoptosis. Mechanistic studies revealed that miR-13896 targets and down-regulates the ATG2A-mediated autophagy pathway, suppressing GC cell growth and metastasis. Furthermore, we enriched hucMSC-sEVs with miR-13896 through electroporation. These engineered EVs specifically targeted tumor sites and significantly reduced GC cell growth and migration in vitro and in vivo. MiR-13896 emerged as a promising therapeutic target for GC. The delivery of miR-13896 via hucMSC-sEVs represents a novel and effective strategy for GC treatment, highlighting the potential of EV-based therapies to combat this malignancy.
Human Hair Follicle Mesenchymal Stem Cell-Derived Exosomes Attenuate UVB-Induced Photoaging via the miR-125b-5p/TGF-β1/Smad Axis
Authors: Cui Hong , Fu Luo-Qin , Teng Yan , He Jun-Jia , Shen Ye-Yu , Bian Qiong , Wang Ting-Zhang , Wang Mei-Xia , Pang Xiang-Wei , Lin Zhi-Wei , Zhu Min-Gang , Cai Yu , Li Yang-Yang , Chen Jin-Yang , Mou Xiao-Zhou , Fan Yi-Bin
Published: December 2024

Cutaneous photoaging, induced by chronic exposure to ultraviolet (UV) radiation, typically manifests as alterations in both the physical appearance and functional properties of the skin and may predispose individuals to cancer development. Recent studies have demonstrated the reparative potential of exosomes derived from mesenchymal stem cells in addressing skin damage, while specific reports highlight their efficacy in ameliorating skin photoaging. However, the precise role of exosomes derived from human hair follicle mesenchymal stem cells (HFMSC-Exos) in the context of cutaneous photoaging remains largely unexplored. We successfully isolated HFMSC-Exos using the ultracentrifugation technique. In cellular experiments, we assessed the migration of human dermal fibroblasts (HDFs) through scratch and transwell assays, evaluated the angiogenesis of human umbilical vein endothelial cells through angiogenesis assays, and examined the expression levels of collagen and matrix metalloproteinase 1 (MMP-1) using Western blotting and quantitative reverse transcription polymerase chain reaction. Furthermore, we established a nude mouse model of photoaging to observe wrinkle formation on the dorsal surface of the animals, as well as to assess dermal thickness and collagen fiber generation through histological staining. Ultimately, we performed RNA sequencing on skin tissues from mice before and after treatment to elucidate the relevant underlying mechanisms. Our findings revealed that HFMSC-Exos effectively enhanced the migration and proliferation of HDFs and upregulated the expressions of transforming growth factor-β1 (TGF-β1), p-Smad2/p-Smad3, collagen type 1, and collagen type 3 while concurrently down-regulating MMP-1 levels in HDFs. Additionally, mice in the HFMSC-Exo group showed quicker wrinkle healing and increased collagen production. HFMSC-Exos miR-125b-5p was demonstrated to reduce skin photoaging by increasing profibrotic levels via TGF-β1 expression. UV-irradiated HDFs and photoaged nude mouse skin showed low TGF-β1 expressions, whereas overexpression of TGF-β1 in HDFs increased collagen type 1, collagen type 3, and p-Smad2/p-Smad3 expressions while decreasing MMP-1 expression. HDFs overexpressing TGF-β1 produced more collagen and altered the Smad pathway. This study demonstrated, both in vitro and in vivo, that HFMSC-Exos increased collagen formation, promoted HDF cell proliferation and migration, and reversed the senescence of UV-irradiated HDFs. TGF-β1 was identified as a target of HFMSC-Exos miR-125b-5p, which controls photoaging via regulating the Smad pathway. The antiphotoaging capabilities of HFMSC-Exos may occur via the miR-125b-5p/TGF-β1/Smad axis, suggesting a promising therapeutic approach for treating skin photoaging.
High-Throughput Screening of 3-Dimensional Co-culture Hair Follicle Mimetic Tissue with an Enhanced Extracellular Matrix for the Screening of Hair Growth-Promoting Compounds
Authors: Pham Huyen T. M. , Kim Hyo-Sop , Nguyen Duc Long , Joo Hyun Woo , Kim Min Kyu , Sung Young Kwan , Vu Minh Hung , Hahm Heung Sik , Kim Woo Jung , Kim Jae-Ho , Park Hyun-Ji
Published: December 2024

Hair follicle cells reside within a complex extracellular matrix (ECM) environment in vivo, where physical and chemical cues regulate their behavior. The ECM is crucial for hair follicle development and regeneration, particularly through epithelial–mesenchymal interactions. Current in vitro models often fail to replicate this complexity, leading to inconsistencies in evaluating hair loss treatments. Advanced 3-dimensional (3D) culture systems that better mimic in vivo ECM dynamics are needed for more effective therapeutic assessments. Here, we introduce a 3D co-culture system designed to replicate in vivo ECM dynamics. The system incorporates primary dermal papilla cells from human patients, co-cultured with neonatal keratinocytes. This platform facilitates uniform spheroid formation through cell sliding and aggregation, enabling the evaluation of approximately 60 spheroids per well. The model is optimized for high-throughput screening, allowing precise assessments of hair-loss-inducing compounds under consistent conditions. We successfully generated dermal papilla cell and keratinocyte spheroids that closely resemble the native ECM structure, providing an optimal microenvironment for studying hair follicle biology. The 3D co-culture model supported efficient spheroid formation with consistent cellular organization and polarization, along with enhanced ECM-related gene expression crucial for hair follicle regeneration. Uniform spheroid formation and reproducibility were demonstrated across experiments. Overall, the novel 3D co-culture system provides a robust platform for replicating in vivo-like ECM conditions, enabling effective assessment of potential hair loss treatments through epithelial–mesenchymal interactions. Its high-throughput capacity, combined with reproducibility and ease of use, makes it a valuable tool for screening therapeutic candidates and advancing hair loss treatment development.
Rational Design of Li
3
V
2
(PO
4
)
3
/C for Phosphate-Based Symmetric Full-Cell Li-Ion Batteries
Authors: Yoo Ye-Wan , Oh Hyeong-Seok , Lee Jong-Kyu , Yoon Jung-Rag , Lee Seung-Hwan
Published: December 2024

To further increase the energy density of lithium-ion batteries (LIBs), various researches have been conducted on high-voltage and high-capacity cathode materials. In this perspective, monoclinic Li
3
V
2
(PO
4
)
3
is a promising candidate due to its promising theoretical discharge capacity of 197 mAh/g with complex phase transition in the voltage range of 3.0 to 4.8 V. However, such asymmetric phase transition behavior with 3 Li
+
ion extraction/insertion is highly irreversible, resulting in an initial discharge capacity of 163 mAh/g with deteriorated capacity retention. We suggest that cycling Li
3
V
2
(PO
4
)
3
in the voltage range of 3.0 to 4.5 V suppresses the irreversible phase transition and elution of transition metal. Hence, Li
3
V
2
(PO
4
)
3
in the voltage range of 3.0 to 4.5 V delivers an initial discharge capacity of about 142 mAh/g and exhibits extremely long cycle retention (78.70% 2,000 cycles), as when cycling in the voltage range of 3.0 to 4.3 V (81.67% 2,000 cycles). Furthermore, we present the possibility of a Li
3
V
2
(PO
4
)
3
||Li
3
V
2
(PO
4
)
3
symmetric all-solid-state battery based on an N/P ratio and a cutoff voltage design, which is demonstrated in liquid electrolyte half-cells and symmetric full cells.
Pyruvate Abundance Confounds Aminoglycoside Killing of Multidrug-Resistant Bacteria via Glutathione Metabolism
Authors: Xiang Jiao , Tian Si-qi , Wang Shi-wen , Liu Ying-li , Li Hui , Peng Bo
Published: December 2024

To explore whether the metabolic state reprogramming approach may be used to explore previously unknown metabolic pathways that contribute to antibiotic resistance, especially those that have been neglected in previous studies, pyruvate reprogramming was performed to reverse the resistance of multidrug-resistant
Edwardsiella tarda
. Surprisingly, we identified a pyruvate-regulated glutathione system that occurs by boosting glycine, serine, and threonine metabolism. Moreover, cysteine and methionine metabolism played a key role in this reversal. This process involved pyruvate-depressed glutathione and pyruvate-promoted glutathione oxidation, which was attributed to the elevated glutathione peroxidase and depressed glutathione reductase that was inhibited by glycine. This regulation inhibited reactive oxygen species (ROS) degradation and thereby elevated ROS to eliminate
E. tarda
. Loss of
metB
,
gpx
, and
gor
of the metabolic pathways increased and decreased resistance, respectively, both in vitro and in vivo, thereby supporting the hypothesis of a pyruvate–cysteine–glutathione system/glycine–ROS metabolic pathway. The role of this metabolic pathway in drug resistance and reprogramming reversal was demonstrated in laboratory-evolved gentamicin-resistant
E. tarda
and other clinically isolated multidrug- and carbapenem-resistant pathogens. Thus, we reveal a less studied antibiotic resistance metabolic pathway along with the mechanisms involved in its reversal.
Maternal Milk Orchestrates the Development of Infant Gut Microbiota: Implications for Health and Future Research Directions
Authors: Wang Ran , Sun Siyuan , Zhang Qi , Wu Guanglei , Ren Fazheng , Chen Juan
Published: December 2024

Human breast milk serves as a vital source of nutrition for infants, and it also plays a critical role in shaping the infant gut microbiota and establishing intestinal homeostasis. This process substantially impacts immune function, neurodevelopment, and overall health. The noninvasive nature of breast milk collection makes it an ethical and accessible area for research, positioning it as a key focus for future studies. These future directions include the identification of novel bacteria combination, the establishment of comprehensive databases on infant microbiota, and the use of computational models to predict interactions between breast milk components and the gut microbiome. Additionally, the creation of diverse biological models and the establishment of infant stool banks will further enhance understanding of host–microbiome interactions and support disease prevention strategies.
Strawberry (Fragaria L.) transportation model in Mexico for Closed Market
Authors: Quintero-Ramírez Juan Manuel , Omaña-Silvestre José Miguel , García-Rico Perla Araceli
Published: December 2024

The objective of this research is to develop a transportation model for the optimization of strawberry distribution in Mexico in a closed market through the use of linear programming. The formulation of this model includes the
objective function and supply and demand constraints derived from various calculations and research. The information gathered comes from official sources such as SIACON, INEGI and the Foreign Trade Information Cube of the Bank of Mexico. On the other hand, transportation costs are calculated using GlobalMap© and the resolution of the model through LINDO 6.1® software. This model allows for the identification of optimal routes and quantities for distribution between strawberry supplying and demanding states, and not only optimizes internal logistics, but also reduces transportation costs.
PERSONNEL MANAGEMENT AS A FACTOR IN THE EFFICIENCY OF A MEDICAL INSTITUTION
Authors: Vasiuk Nataliia , Petruchenko Nataliia , Dankevich Nataliia
Published: December 2024

The article analyzes the theoretical and practical aspects of the personnel management system in a medical institution (based on the example of the "Primary Health Care Center No. 2" of the Sviatoshynskyi District in Kyiv) and provides practical recommendations for improving management processes to enhance the productivity of the medical institution and the quality of healthcare services.
The problems of the medical institution have been identified, including understaffing of the facility; a low level of staffing with medical positions (mainly in specialized fields); the absence of personnel planning, employee evaluation, career planning, and the development of a personnel reserve, as well as a lack of on-the-job training; a formalized approach to recruitment and dismissal procedures; lack of automation in personnel management functions; inefficiency in the structure of the HR department, outdated name of this unit; insufficient attention to developing an effective employee motivation system, and other issues.
The article proposes measures to improve the personnel management system at the "Primary Health Care Center No. 2" of the Sviatoshynskyi District in Kyiv. The implementation of these measures aims to enhance the personnel management system and is focused on improving the performance of medical staff, the quality of healthcare services, reducing staff turnover, and improving the image and competitiveness of the medical institution.
In order to improve personnel management at the "Primary Health Care Center No. 2" of the Sviatoshynskyi District in Kyiv, the article highlights the following key strategic directions to be achieved in the implementation of the Personnel Security Strategy: activation of motivational mechanisms; enhancement of the training and development system for medical staff; and reorganization of the HR department.
CHANGE MANAGEMENT METHODOLOGY: SINGO MODEL
Authors: Kashchuk Kateryna
Published: December 2024

The article examines and summarizes information about the work and effectiveness of the model of Japanese scientific engineer Shigeo Shingo (1909–1990) Questions regarding the prerequisites for its emergence, development, and implementation in the management of Japanese enterprises and companies are addressed, as well as the potential application of this model in Ukrainian enterprises. The value system at enterprises is analyzed in the context of the Shingo model and its role in the involvement of employees in the process of transformation of production. The Shingo model is used to analyze the role of employees in the process of transformation of production. In the present circumstances of the transition of Ukrainian enterprises to a highly volatile stage of development, the significance of the subject matter is undeniable.
Value orientations and authentic models of employee behavior of developing enterprises become topical in the context of the need to move towards high productivity. It is therefore important to assess the degree of readiness of the economy for the transformation process, its ability to adapt to changes, and its potential speed of introduction of technologies and innovations. In this work, the effective structure of the production transformation process and the individual elements of the transformation are indicated. It is demonstrated the impact of innovative methods and methods of transformation on the speed of growth and development of productivity at enterprises. The material presented in the article encompasses significant aspects of the fundamental essence of the innovative nature of development based on the Shingo model.
Keefektifan Model Project Based Learning Terhadap Motivasi Dan Hasil Belajar Peserta Didik Kelas XI MIPA 1 SMAN 1 WONOMULYO (Studi pada Materi Pokok Larutan Penyangga)
Authors: Jasman Mardhatillah , Djangi Muhammad Jasri , Syahrir Muhammad
Published: December 2024

ABSTRAK
Penelitian ini bertujuan untuk mengetahui keefektifan model project based learning untuk meningkatkan motivasi dan hasil belajar peserta didik pada materi pokok larutan penyangga. Desain penelitian ini adalah one-group pretest-posttest design dengan subjek penelitian kelas XI MIPA 1 SMAN 1 Wonomulyo yang berjumlah 31 orang. Variabel bebas dalam penelitian ini adalah model project based learning. Variabel terikatnya adalah motivasi dan hasil belajar peserta didik. Teknik pengumpulan data adalah pemberian tes awal (pretest) dan tes akhir (posttest). Data penelitian ini dianalisis menggunakan uji N-Gain dan didapatkan hasil nilai N-gain motivasi belajar sebesar 0,4 (kategori sedang) dan nilai N-Gain untuk hasil belajar peserta didik sebesar 0,72 (kategori tinggi) dilanjutkan dengan uji statistik inferensial hasilnya t hitung lebih besar dari 1,697 dengan tingkat kepercayaan 5%. Dari hasil ini dapat diketahui bahwa model project based learning efektif untuk meningkatkan motivasi dan hasil belajar peserta didik.

ABSTRACT
The purpose of this study is to determine the effectiveness of the project-based learning model in increasing motivation and learning outcomes of students on the subject of buffer solutions.The research design is one group pre and posttest design which involved 31 students in class XI MIPA 1 at SMAN 1 Wonomulyo as the subject.The independent variable in this study is the project-based learning model while the dependent variable is students' motivation and learning outcomes. The data collection technique is the administration of an initial test (pretest) and a final test (posttest) and is analyzed using the N-Gain test. The results obtained were the N-Gain value of learning motivation 0.4 (moderate classification) and the N-Gain value of students' learning results 0.72 (high classification) followed by inferential statistical test with the value of r count more than 1,697 with a confidence level 5%. From these results, it can be seen that the project based learning model is effective in increasing student motivation and learning outcomes.
USO DO TAPING NA REABILITAÇÃO FISIOTERAPÊUTICA DAS COMPLICAÇÕES PÓS-OPERATÓRIAS DE MASTECTOMIA: REVISÃO DE LITERATURA
Authors: Bruno Moura Germano João , Braga Rolim Vieira Renata , Jorge Dias Michel , Cristian Alves de Sousa Kennedy
Published: December 2024

Introdução: O câncer de mama é um dos tipos de câncer com maior índice de mortalidade no mundo, sendo caracterizado pelo crescimento ou multiplicação rápida e desalinhada das células presentes na mama. A fisioterapia possui vários meios de intervenção, inclusive o taping, que visam tratar e prevenir as complicações decorrentes do tratamento oncológico da mama, podendo serem elas o surgimento da síndrome da rede axilar, dor, diminuição da amplitude de movimentos e linfedema. Objetivo: Apresentar a eficácia do uso do taping no tratamento fisioterapêutico de complicações pós-operatórias em pacientes que realizaram cirurgia de mastectomia. Métodos: Trata-se de uma revisão de literatura, na qual foram coletados dados de estudos inseridos nas bases de dados indexadas Scientific Eletronic Library Online (SciELO) e National Center for Biotechnology Information (PUBMED). A busca foi realizada entre os meses de agosto a outubro de 2024 utilizando a seguinte combinação de descritores: Fisioterapia. Mastectomia. Pós-operatório. Taping devidamente cadastrados no Descritores em Ciências da Saúde (DECS). Foram selecionados artigos publicados entre os anos de 2010 a 2024, estando eles relacionados ao objetivo desse estudo. Resultados: O taping posssui efeitos positivos sobre a síndrome da rede axilar, o linfedema, a dor e a diminuição da amplitude de movimentos. Estudos com amostras maiores e protocolos mais rigorosos são necessários para validar as intervenções e refinar as estratégias de reabilitação. Conclusão: Com base nas evidências apresentadas, conclui-se que o uso do taping como estratégia de reabilitação pós-mastectomia apresenta resultados promissórios, promovendo efeitos benéficos, principalmente quando associado à outras técnicas como liberação miofascial, drenagem linfática, terapia manual e eletrotermofototerapia. A técnicas ainda requer investigações mais aprofundadas para validar seus prazos, padronizar métodos de aplicação e efeitos a longo prazo.

Palavras-chave: Fisioterapia; Mastectomia; Taping.
Formulation Design and In Vitro Evaluation of Dutasteride Orodispersible Tablets Using super disintegrants
Authors: Pasha Shaik Khaja , Priya R. Venu , Panda Niranjan , Farhath Fathima Ayesha
Published: December 2024

Benign prostatic hyperplasia(BPH), also known as
enlarged prostate symptoms in males, is treated
with dutasteride. The liver substantially
metabolizes dutasteride. Because it skips
metabolism, the orodispersible tablet was a good
dose to overcome these problems. The BCS
categorization for dutasteride is class II. The
current research attempted to design and develop
an orodispersible tablet containing dutasteride
using croscarmellose sodium, povidone, and
sodium starch glycolate as superdisintegrants. This
was done to achieve rapid disintegration when held
beneath the tongue, which would allow for direct
absorption of the active ingredient by the oral
mucosa, thereby avoiding fast-pass metabolism and
increasing the bioavailability. Wet granulation
techniques were used to create dutasteride
orodispersible granules, and the evaluation of all
precompression parameters fulfilled the acceptance
standards, demonstrating the granules' outstanding
flow characteristics. Different post-compression
characterizations of the tablet were conducted, and
the results complied with pharmacopeia
requirements. In vitro, release studies were carried
out with dissolution equipment of the USP II
paddle type for a variety of different formulations.
Investigations into the in vitro release kinetics of
both the zero-order and the first-order kinetic
models were carried out. FTIR tests revealed that
the drug and the excipients were not incompatible
with one another. Following the foregoing
formulations, the drug and polymers are thermally
stable, according to DSC investigations that were
conducted to determine the thermal stabilities of
the drug and the physical mixture of the drug and
excipients employed in formulation. To verify the
stability of dosage forms, accelerated stability
studies were conducted, and the optimized
formulation was discovered to be stable to an
acceptable range.
A Review On Phytochemical and Anti Inflammatory Activity of Plant Extract
Authors: Nair Abhinand M , Parveen Liya , Mol M Raheena , Asif Shaheer P K Mohammed , Kattupparuthi Siraj , K.V, Dr. Kavitha , Dr.Sirajudheen.M.K Dr.Sirajudheen.M.K
Published: December 2024

In the last few years, the journey through plant
products as a major source of anti-inflammatory
agents have caught attention in biomedical
researches.Plant extracts have emerged as
promising candidates due to their rich
phytochemical composition and traditional
medicinal uses. This literature review aims to
synthesize current knowledge on the antiinflammatory properties of plant extracts, focusing
on their mechanisms of action, bioactive
constituents, and therapeutic potentials. The review
systematically examines findings from
experimental studies and clinical trials,
highlighting the diversity of plant sources and their
efficacy in modulating inflammatory pathways.
Additionally, challenges such as standardization of
extracts and variability in bioactivity are discussed,
emphasizing the need for detailed scientific
validation to translate these findings into clinical
applications. Overall, this review explains the
potential of plant extracts as valuable resources for
developing novel anti-inflammatory therapies,
while proving for further research to elucidate their
full therapeutic benefits
KEBUTUHAN MEDIA LITERASI KESEHATAN UNTUK PENCEGAHAN SINDROM METABOLIK: STUDI KUALITATIF PADA PEKERJA SEKTOR FORMAL
Authors: Samsiana Samsiana , Basri K. Sarinah , Purimahua Sintha Lisa , Syakur Rosdiana
Published: December 2024

Latar Belakang: Sindrom metabolik sering muncul akibat gaya hidup modern dan kebiasaan tidak sehat seperti rutinitas duduk lama di depan komputer yang umum di kalangan pekerja kantoran. Penting untuk melakukan upaya pencegahan melalui intervensi literasi kesehatan yang dapat mendorong adopsi gaya hidup sehat. Penelitian ini mengeksplorasi pengetahuan tingkat literasi kesehatan, kebutuhan media literasi kesehatan pekerja, serta upaya kesehatan apa saja yang telah dilakukan dalam upaya pencegahan sindrom metabolik.Metode: Penelitian kualitatif ini melibatkan 11 pekerja dan 2 pegawai kesehatan yang dipilih secara purposive sampling. Data dikumpulkan melalui wawancara mendalam menggunakan panduan wawancara dan dianalisis dengan pendekatan content analysis.Hasil: Sebanyak sepuluh pekerja, satu pimpinan pekerja, satu pengelola PTM Dinas Kesehatan, dan satu petugas kesehatan puskesmas terlibat dalam penelitian ini. Analisis wawancara mengidentifikasi empat tema: pengetahuan pekerja tentang sindrom metabolik, tingkat literasi kesehatan, upaya pencegahan sindrom metabolik, dan kebutuhan media literasi kesehatan.Kesimpulan: Edukasi melalui media sosial dan situs web sangat penting untuk menjangkau lebih banyak pekerja. Peningkatan literasi kesehatan berbasis teknologi dan informasi yang mudah diakses diperlukan agar pekerja lebih proaktif dalam mencegah dan mengelola sindrom metabolik. Organisasi tempat kerja diharapkan menyediakan program kesehatan yang terstruktur dan wajib diikuti oleh seluruh pekerja untuk meningkatkan pemahaman dan keterampilan dalam mengadopsi pola hidup sehat sehingga dapat mengurangi risiko sindrom metabolik dan meningkatkan kualitas hidup pekerja. Kata kunci :  Literasi Kesehatan, Pencegahan, Sindrom Metabolik
Disk array-type plasmonic chip for fluorescence enhancement
Authors: Nawa Yasunori , Shimosaka Riku , Shimizu Atsushi , Tawa Keiko
Published: December 2024

Plasmonic chips are chip-type devices with wavelength-order periodic structures covered with metal based on grating-coupled surface plasmon resonance for sensitive detection. In this study, we investigated a disk-array-type (DA) structure, which is an inversion of the hole-array-type (HA) structure, to further improve the fluorescence enhancement. Increases in the dip depth in the reflection spectra and the corresponding field enhancement were observed for the DA structure, resulting in larger fluorescence enhancement than that of the HA structure. The relationship between the duty ratio, convex height, and enhancement factors was also investigated. The contribution of excitation enhancement was dominant and strongly correlated with dip depth, reaching a maximum at a convex height of 25 nm and a duty ratio of approximately 0.6. Furthermore, the excitation enhancements calculated by rigorous coupled wave analysis showed an optimal structure with the highest values.
Effect of random potential on two-dimensional electronic states
Authors: Mori Nobuya
Published: December 2024

It has been observed that the subthreshold coefficients of MOSFETs saturate at cryogenic temperatures. One model that explains this phenomenon is one that assumes an exponential tail in the density of states (DOS) of a two-dimensional electron gas (2DEG). In this study, the effect of a random potential on 2DEG states has been investigated by calculating the DOS within the effective mass approximation. It is shown that the spatially averaged DOS has exponential tail states in lower energy regions below an onset energy. The dependence of the tail width and the onset energy on the strength and the correlation length of the potential fluctuation is presented.
Influência do nível de bateria e da distância sobre a intensidade de luz emitida por aparelhos fotopolimerizadores
Authors: Ribeiro Rafael Antonio de Oliveira , Guimarães Renata Pedrosa , Nascimento Alexandre Batista Lopes do , Teixeira Hilcia Mezzalira
Published: December 2024

Objetivo: O objetivo deste estudo foi analisar a influência do nível de bateria e da distância da fonte sobre a intensidade de luz dos aparelhos fotopolimerizadores. Métodos: Foram analisados 11 fotopolimerizadores Optilight Max – Gnatus utilizados nas clínicas escola de uma instituição pública na cidade do Recife. Inicialmente foi observado que os aparelhos realizavam um total aproximado de 310 ciclos de polimerização de 20 segundos no modo constante, dessa forma, 155 ciclos eram necessários para atingir 50% do nível de bateria. Em seguida, foram realizadas as aferições da intensidade de luz emitida pelos aparelhos em 100% e 50% do nível de bateria nas distâncias de D1: 0mm; D2: 2mm; D3: 4mm; D4: 6mm e D5: 8mm, padronizadas por uma matriz de acrílico. Resultados: Através dos resultados foi possível observar diminuição da intensidade de luz de aproximadamente 24,09% quando o aparelho estava com sua bateria em 50%, além disso, foi observado a diminuição gradual da intensidade de luz com a variação da distância da ponta do aparelho até o radiômetro. Essa diminuição variou de 49,15% até 76,99% com nível de bateria em 100% e 49,67% até 78,15% com nível de bateria em 50% (ANOVA/Tukey; α=5%). Conclusão: Através desse estudo observou-se que o nível de bateria é capaz de influenciar na intensidade de luz de aparelhos fotopolimerizadores, além disso, maiores distância até o sensor do radiômetro podem agir em sinergia para uma diminuição ainda mais significativa. Níveis mais baixos de bateria associados a maiores distâncias resultam em níveis consideravelmente mais baixos de intensidade de luz.
Comparative evaluation of smear layer removal efficacy using various final irrigation protocols: a SEM study
Authors: Dorilêo Maura Cristiane Gonçales Orçati , Guedes Orlando Aguirre , Moussa Letícia Junqueira de Pádua Sesti Gomes , Estrela Cyntia Rodrigues de Araújo , Oliveira Helder Fernandes de , Aranha Andreza Maria Fábio , Gonini Junior Alcides
Published: December 2024

Objective: This study aimed to assess the efficacy of 0.2% Chitosan and 17% EDTA, with or without ultrasonic activation, in removing the smear layer (SL). Methods: Fifty bovine incisors were sectioned 17 mm from their apices, instrumented using ProTaper Universal instruments and divided into five groups (n=10): I- Distilled water + conventional irrigation (CI) (control), II- 17% EDTA + CI, III- 0.2% Chitosan + CI, IV- 17% EDTA + passive ultrasonic irrigation (PUI) and V- 0.2% Chitosan + PUI. The roots were split longitudinally and examined under a scanning electron microscope at 500x magnification to assess SL removal. SL presence was scored by three blinded examiners using a five-point scale. Data was analyzed using Kruskal-Wallis and Mann-Whitney U-tests (p<0.05). Results: The statistical analysis revealed significant differences in SL removal among groups (p<0.05). Group IV (EDTA + PUI) exhibited significantly higher removal efficacy than other groups (p<0.05). SL removal was highest in the cervical third compared to middle and apical thirds. Conclusions: EDTA combined with PUI demonstrated superior SL removal.
A PRESCRIÇÃO VIRTUAL
Authors: Lagrota Marcelo
Published: December 2024

O presente estudo busca abordar a aplicabilidade da Prescrição Virtual no Direito Penal Brasileiro. O tema constitui o padrão de discussão dos tribunais brasileiros em relação às controvérsias doutrinárias acerca de sua admissibilidade. Pretende-se, a partir de uma análise principiológica e hermenêutica do Direito, Lei de Introdução às normas do Direito Brasileiro, bem como dos critérios legais extraídos do Código Penal para a aplicação da pena, conferir sua aplicabilidade para aprimorar a gestão de recursos humanos, materiais e tempo na justiça criminal.
РЕЗУЛЬТАТИ ДОСЛІДЖЕННЯ БАКТЕРІОЛОГІЧНИХ ЗМІН У ВОДОНОСНИХ ГОРИЗОНТАХ В УМОВАХ ПІДВИЩЕННЯ ТЕМПЕРАТУРИ
Authors: Барило А. А. , Морозов Ю. П. , Бачеріков О. В. , Лобанова І. В.
Published: December 2024

Акумулювання надлишкової та технологічної теплоти у верхніх водоносних горизонтах призводить до теплового та бактеріального забруднення горизонтів. У статті наведено результати експериментальних досліджень щодо бактеріологічних змін у водоносних горизонтах в результаті підвищення їх температури. Дослідження проведено на основі аналізу проб підземних вод полтавського водоносного горизонту з експлуатаційних свердловин, що розташовані на експериментальному полігоні ІВЕ НАНУ. Було визначено хімічний та бактеріологічний склад вод та рівень їх природної забрудненості. Підземні води належать до групи слабомінералізованих гідрокарбонатних кальцієво-магнієвих вод із середньою мінералізацією 495,86 мг/дм3 і мають незначне забруднення мезофільними бактеріями. В лабораторії Центру превентивної медицини Державного управлення справами було проведено аналізи, які дозволяють змоделювати поведінку бактерій залежно від підвищення температури підземної води в горизонті. Встановлено, що підвищення температури води у водоносному горизонті в інтервалі температур 20-40 °C призводить до росту кількості бактерій в 30−40 разів. Отримані результати вказують на необхідність передбачення використання методів бактеріологічного очищення під час створення систем підземного акумулювання тепла.
THE EFFECTS OF NANOADDITIVES ON A SINGLE CYLINDER DIESEL ENGINE USING PROSOPIS JULIFLORA BIODIESEL
Authors: Аруначалам В. , Навенчандран П. , Балу П.
Published: December 2024

This study looks at how a Prosopis Juliflora methyl ester-powered one-cylinder direct injection diesel engine burns, works, and gives off emissions when titanium nanoadditives are added at two different concentrations: 25 ppm and 50 ppm. The test engine should be run at 1500 rpm continuously with 17.5 compression and 23-degree BTDC timing. A magnetic agitator and an ultrasonic converter are used to mix aluminium nano-additive materials. Due to the high surface-to-area-volume ratio of alumina particles with smaller-scale alumina nanometrics, combustion is boosted and emissions are minimised, according to experimental studies. Furthermore, the alumina blend greatly improved brake thermal efficiency, lowering fuel consumption by 6.56 percent initially and 7.37 percent thereafter. In terms of combustion efficiency, this leads to a modest reduction in NOx, CO, HC, and smoke emissions.