bluuebunny/crossref_metadata_2025 huggingface dataset:
The influence of suturing and/ gluing of perforated Schneiderian membrane during sinuslift procedure on the outcome- a retrospective study
Authors: Khoury Fouad , Schmidt Christoph , Jackowski Jochen
Published: October 2024

The sinus lift procedure has become the most common method for maxillary bone augmentation. The most frequently observed intraoperative complication is the perforation of the Schneiderian membrane. Various treatment options have been proposed for managing these perforations, including the use of resorbable membranes, centrifugated blood products as PRF, or PRGF, suturing, and fibrin glue application. While long-term studies exist for the use of resorbable membranes to close perforations, there is limited data on the long-term outcomes of suturing or gluing the perforated sinus membrane.
The aim of this retrospective study is to evaluate the long-term outcomes of suturing and/or applying fibrin glue to repair perforated sinus mucosa during sinus floor elevation procedures. Between 2005 and 2009, a total of 692 patients underwent 923 sinus lift surgeries, and Schneiderian membrane perforation occurred in 202 sinus floor elevations (21.98%) across 168 patients. The main documented causes of perforations, which ranged from 2 to 10 mm in diameter, were the presence of septa, followed by thin and adherent membranes. Of the perforations, 100 (49.5%) were treated with microsurgical suturing combined with fibrin glue, 78 (38.6%) were treated with fibrin glue alone, and 24 (11.9%) were treated exclusively with suturing.
Sinus grafting was performed using autogenous bone in combination with a biomaterial, following the layering technique. All surgeries resulted in primary healing without complications, enabling all patients to undergo restoration as planned. The long term clinical and radiological evaluations of 44 randomly selected patients who followed the recall program up to 10 years post operative confirmed the effectiveness of this treatment approach.
Sleep and Risk of Peptic Ulcer Disease: a cross-sectional study
Authors: Ren Qian , Gao Jie , Li Bowen , Luo Xiu , Zhao Guangyuan , Zheng Ya , Yang Fucheng , Ji Rui , Guo Qinghong , Chen Zhaofeng , Wang Yuping , Lu Hong , Zhou Yongning
Published: October 2024

BACKGROUND & AIMS：The association between sleep duration and gastrointestinal disorders has been investigated extensively, but the specific link with peptic ulcer disease (PUD), including gastric ulcer (GU) and duodenal ulcer (DU), has not been thoroughly examined. Our objective was to assess the relationship between sleep duration and the occurrence of PUD, GU, and DU.
METHODS: We undertook a cross-sectional analysis involving 4,860 individuals from the Wuwei cohort. Participants' daily sleep duration was assessed by inquiring about the number of hours slept per day. Odds ratios and 95% confidence intervals were calculated using binary logistic regression.
RESULTS: In age- and sex-adjusted logistic regression analyses, those who slept > 9h were less likely to develop GU compared to those who slept 7-9h. (OR = 0.67, 95% CI= 0.55-0.80) , but had an increased risk of developing DU (OR = 1.39, 95% CI=1.10-1.77).This result remains significant in Model 2.
CONCLUSIONS: Prolonged sleep associated with gastric ulcer and duodenal ulcer disease
Biomarker-Based Prediction of Ischemic Stroke in Patients With H-type Hypertension
Authors: Chen Ke , He Jianxun , Fu Lan , Song Xiaohua , Cao Ning , Yuan Hui
Published: October 2024

Hypertension combined with hyperhomocysteinemia significantly raises the risk of ischemic stroke. Our study aimed to develop and validate a biomarker-based prediction model for ischemic stroke in H-type hypertension patients. We retrospectively included 3,305 patients in the development cohort, and externally validated in 103 patients from another cohort. Logistic regression, LASSO regression, and best subset selection analysis were used to assess the contribution of variables to ischemic stroke, and models were derived using four machine learning algorithms. Area Under Curve (AUC), calibration plot and decision-curve analysis (DCA) respectively evaluated the discrimination and calibration of four models, then external validation and visualization of the best-performing model. There were 1,415 and 42 patients with ischemic stroke in the development and validation cohorts. The final model included 8 predictors: age, antihypertensive therapy, biomarkers (serum magnesium, serum potassium, proteinuria and hypersensitive C-reactive protein), and comorbidities (atrial fibrillation and hyperlipidemia). The optimal model, named A2BC ischemic stroke model, showed good discrimination and calibration ability for ischemic stroke with AUC of 0.91 and 0.87 in the internal and external validation cohorts. The A2BC ischemic stroke model had satisfactory predictive performances to assist clinicians in accurately identifying the risk of ischemic stroke for patients with H-type hypertension.
Efficacy of Venetoclax combined with Homoharringtonine and Cytarabine for Younger Adults with Newly Diagnosed AML
Authors: Song Bao-Quan , Liu Yin , Kong Xin , Pu Yan , Zhang Jian , Wu De-Pei , Qiu Hui-ying
Published: October 2024

Venetoclax (VEN) based induction therapy have shown a promising treatment for patients with acute myeloid leukemia (AML). However, the optimal VEN combination is urgently needed. We aimed to investigate the effective and tolerability of venetoclax combined with homoharringtonine plus cytarabine (VHA) regimen for newly diagnosed AML. We retrospectively analyzed the outcomes of 55 ND AML patients treated with VHA regimen. The overall response rate (ORR) was 92.7% (51/55, 95% CI 82%-98%). The composite complete remission (CRc) rate was 87.3% (48/55, 95% CI 76%-95). 44 (91.7%, 95% CI 67-90) of 48 patients who reached complete remission (CR) and measurable residual disease (MRD)-negative was attained in 85.4% of CRc patients (41/48, 95% CI72-94). ORR and CRc were 95% (19 of 20 patients)[95% CI 75%-100%] and 75% (15 of 20 patients) [95% CI 51%-91%] in patients with adverse risk. The most common grade 3-4 adverse events were febrile neutropenia (32.7%), pneumonia (16.3%) and sepsis (9.1%). Median overall survival (OS) was 26 months and event-free survival (EFS) was not reached. 1-year overall survival was 83%, 1-year event-free survival was 82%. These preliminary data suggest that the VHA regimen leads to a very high rate of CR and low toxicity. Especially for adverse risk patients, it provide an opportunity for allogeneic haematopoietic stem cell transplantation (allo-HSCT).
Strategies for Management of Posterior Deciduous and Young Permanent Teeth with Developmental Defects of Enamel - Systematic Review and Meta-analysis
Authors: Tedesco Tamara , Pascareli-Carlos Aline , Ribeiro Rayane , Souza Beatriz , Floriano Isabela , Diniz Michele , Bezerra Sávio , Silva Erika , Raggio Daniela , Novaes Tatiane
Published: October 2024

Objectives: To assess the management strategies for treating deciduous and young permanent teeth with Developmental defects of enamel (DDE) presenting post-eruptive breakdown or atypical cavitated carious lesions.
Methods: Electronic databases searches were conducted up to May 31, 2023, identifying clinical studies related to the treatment strategies for posterior deciduous or young permanent teeth with DDE in children up to 14 years old. Results: Sixteen studies were included: 12 focusing on molar incisor hypomineralization (MIH), 1 on hypomineralization, 1 on imperfect amelogenesis, and 2 incorporating different types of DDE. Esthetic Crowns, Metal Crowns, Composite Resin (CR), Glass Ionomer Cement (GIC), and Metal and CR Onlays were the most used restorative treatments. Only similar studies for MIH were found, and meta-analyses were conducted. Over a 24-month follow-up period, a proportion of success rate of 96% (95% CI 92%-100%) was identified. Subgroup analysis did not reveal a difference among the restorative treatments (p = 0.922).
Conclusion: Aesthetic and metal crowns, CR, and GIC can be used with high longevity. However, the certainty of evidence is low, and most studies exhibited a high risk of bias.PROSPERO database CRD42021204415
Superselective embolization of the segmental artery of a ruptured horseshoe kidney: a case report
Authors: Dimitrić Žarko , Petreš Andrej , Vojinov Saša , Jeremić Dimitrije , Lakić Tanja , Govedarica Srđan
Published: October 2024

Background:
The horseshoe kidney (HSK) is the most common upper urinary system congenital defect, and blunt force injury accounts for 19% of kidney injuries in people with preexisting renal diseases.
Case presentation:
A 73-year-old patient, who was involved in a road traffic accident, was admitted to the Emergency department. А computed tomography (CT) trauma scan revealed a ruptured horseshoe kidney after which superselective embolization was successfully performed.
Conclusions
Uncommon emergencies such as this present difficulty in selecting the appropriate treatment, with this case report contributing to a potential standardized approach for this kind of emergency.
Four months of treatment with anakinra combined with glucocorticoids for giant cell arteritis: a multicenter, randomized, double-blind, placebo-controlled trial
Authors: de Boysson Hubert , Ly Kim Heang , Geffray Loïk , Quemeneur Thomas , Liozon Eric , Bezanahary Holy , Gouellec Noémie Le , Audemard Alexandra , Dumont Anael , Deshayes Samuel , Boutemy Jonathan , Maigné Gwénola , Silva Nicolas Martin , Sultan Audrey , Mauff Brigitte Le , Petit Gauthier , Parienti Jean-Jacques , Aouba Achille
Published: October 2024

Background
Efficacy and tolerance of anakinra (ANK) in the treatment of giant cell arteritis (GCA) need to be assessed.
Methods
This phase 3 study (NCT02902731) was a prospective multicenter, randomized, double-blind, placebo-controlled trial conducted over a 52-week period. GCA patients were randomized 1:1. From inclusion to week 16 (W16), patients in the anakinra (ANK) group received a daily subcutaneous injection of 100 mg of anakinra, whereas patients in the other group received placebo (PBO). In both arms, glucocorticoid (GC) discontinuation was planned at week 52 (W52). The endpoints were the relapse rates at W16, W26, and W52 and the completion of GC tapering. Given the emergence of the SARS-CoV-2 pandemic, the study was stopped prematurely.
Results
Thirty patients with new GCA diagnoses from 5 centers were randomized as follows: 17 in the ANK group and 13 in the PBO group. During the first 16 weeks, the relapse rates were 12% (n = 2) and 23% (n = 3) in the ANK and PBO groups, respectively (p = 0.63). At week 26, 12 (40%) patients had relapsed: 8 (47%) in the ANK group and 4 (31%) in the PBO group (p = 0.47). At W52, the relapse rate (overall, 50%) did not differ between the ANK group (53%; 9/17 patients) and the PBO group (46%; 6/13 patients) (p = 1). Two patients in each group discontinued GCs (p = 0.87). Seven serious AEs were reported in five patients, including 4 in patients receiving ANK.
Conclusions
Although prematurely discontinued, this study does not support the use of 4 months of treatment with anakinra combined with GCs to reduce the risk of relapse or GC exposure.
Trial registration:
ClinicalTrials.gov NCT02902731
Whole-body visualization of SARS-CoV-2 biodistribution in vivo by immunoPET imaging in non-human primates
Authors: Naninck Thibaut , Detrille Alexandra , Huvelle Steve , Gils Marit J. van , Geara Tatiana , Pascal Quentin , Snitselaar Jonne , Bossevot Laetitia , Cavarelli Mariangela , Bosquet Nathalie , Relouzat Francis , Contreras Vanessa , Chapon Catherine , Caillé Fabien , Sanders Rogier , Grand Roger Le
Published: October 2024

The COVID-19 pandemic has caused nearly 780 million cases globally. While available treatments and vaccines have allowed a reduction of the mortality rate, the spread of the virus is still evolving quickly, resulting in the emergence of new variants. Despite extensive research, the long-term impact of SARS-CoV-2 infection is still poorly understood and requires further investigation.
Routine analysis provides limited access to the tissues of patients, necessitating alternative approaches to investigate viral dissemination in the organism. We addressed this issue by implementing a whole-body in vivo imaging strategy to longitudinally assess the biodistribution of SARS-CoV-2. We demonstrate in a COVID-19 non-human primate model that a single injection of non-neutralizing radiolabeled [89Zr]COVA1-27-DFO human monoclonal antibody targeting a preserved epitope of the SARS-CoV-2 spike protein allows longitudinal tracking of the virus by positron emission tomography with computed tomography (PET/CT). Convalescent animals exhibited a persistent [89Zr]COVA1-27-DFO PET signal in the lungs, as well as in the brain, three months following infection. This imaging approach also allowed detection of the virus in various organs, including the airways and kidneys, of exposed animals during the acute phase of infection. Overall, the technology we developed offers a comprehensive assessment of SARS-CoV-2 distribution in vivo and provides a new approach for the non- invasive study of long-COVID physiopathology.
MiRNA-29b accelerates the PDGF in exosomes and stimulates hepatic stellate cells to promote liver fibrosis in biliary atresia
Authors: Liao Xilin , Liu Rui , Zhou Teng , Zhao Xiaohuan , Xiong Xiangang , Zhang Kaizhi , Zheng Zebing , Xia Xingrong , Tang chengyan , Gong Yuan , Du Qing , Huang Lu , Zhu Daiwei , Zhou Wankang , Liu Yuanmei , Jin Zhu
Published: October 2024

Background
Biliary atresia (BA) is one of the most common liver diseases in infants and young children. Its etiology and pathogenesis are still unclear. The purpose was to investigate the mechanism by which the highly expressed miRNA-29b in exosomes regulates the high expression of PDGF through methylation, hepatic stellate cell activation, and participation in the hepatic fibrosis of BA.
Methods
In previous experiments, peripheral blood exosomes of children with biliary atresia and cholangiectasia (CC) were analyzed, and it was found that miRNA-29b was highly expressed in the exosomes of children with BA. Meanwhile, PDGF was highly expressed.Jurkat cells were infected with lentivirus overexpressing miRNA-29b, and the exosomes of Jurkat cells were extracted and cocultured with hepatic stellate cells (LX-2).
Results
DNMT3a and DNMT3b expression was downregulated and PDGFA expression was upregulated in the miR-29b overexpression group, which activated hepatic stellate cells. DNMT3a/3b was identified as the target gene of miR-29b by luciferase assays. Downregulation of DNMTs resulted in hypomethylation of the PDGF promoter region and high expression of PDGFA. Western blot analysis showed increased expression of COL1A1 and α-SMA, and CCK-8 analysis showed increased proliferation of LX-2 cells.
Conclusions
Our study showed that miRNA-29b was highly expressed in peripheral blood exosomes and that PDGFA was highly expressed in liver tissues in children with BA. Exosomes overexpressing miRNA-29b caused hypomethylation of the DNA promoter region of PDGFA in hepatic stellate cells, promoted the high expression of PDGFA, activated hepatic stellate cells, and participated in BA-associated liver fibrosis, elucidating the pathogenesis of BA.
Qualitative study of experience of patients with external drainage tube out of hospital with biliary and pancreatic system diseases
Authors: LIU Jingli , WANG Qianqian , YE Xiaoping , Xie Lixia , WU Ziyu , HUANG Doudou , YAO Yanrong
Published: October 2024

Objective: This study aimed to understand the experience of patients with external drainage tube out of hospital with biliary and pancreatic system diseases and provide a theoretical basis and reference for formulating nursing intervention measures based on the actual experience of patients with ducts.
Methods: An objective sampling method was used to select patients treated in the Department of Hepatobiliary Surgery and the Pipeline Nursing Outpatient Department of a triple A hospital in Yinchuan City, Ning Xia province, China, and had external drainage tubes in place outside the hospital for more than one month as research objects. Semi-structured interviews were conducted under the guidance of phenomenological research methods, and 17 participants were finally included. This study was reviewed by the Research Ethics Committee of the General Hospital of Ningxia Medical University (Ethics number: KYLL-2022-1341), and all subjects voluntarily participated in this study.
Results: Four themes and ten sub-themes were extracted, including negative experiences in social life (reinforcement of patient role and absence of family role, changes in work, life, and lifestyle), physical and mental discomfort experience (negative psychological experience and physical discomfort experience), lack of related knowledge (lack of disease-related knowledge, lack of pipeline nursing skills, and weak consciousness). and hope to get help (obtain the appropriate size of the drainage bag, improve fixed film, and the follow-up method).
Conclusion: The patients’ experience of patients with drainage tube outside of hospital for hepatobiliary and pancreatic system diseases is primarily discomfort. There are some discomfort experiences and a lack of relevant-disease knowledge, and patients are eager to get professional help from the medical staff.
Lower Water-soluble Vitamins and Higher Homocysteine Levels Contribute to Cognitive Decline in Patients with Neurodegenerative Diseases: a retrospective case-control study
Authors: Zhang Cuiping , Hu Yao , Cao Xinyi , Deng Yuhang , Wang Yuting , Wu Xiaoyan , Jiang Haoqin
Published: October 2024

Background: Evidence of the effectsof water-soluble vitamins (e.g., vitaminB, vitamin C, and folic acid [FA]) on cognitive function in patients with neurodegenerative diseases is mixed. Furthermore, the relationships among homocysteine (Hcy) metabolism, water-soluble vitamins, and cognitive decline remain unclear. Therefore, we aimed to investigate the role of the levels of water-soluble vitamins (e.g., vitamins B1, B2, B3, B5, B6, B9, B12, and C and FA) and Hcy in dementia progression in patients with neurodegenerative diseases.
Methods: In this retrospective cohort study, we enrolled 280 healthy controls and 646 patients with a neurodegenerative disease. Patientswere classified into a Parkinson’s disease (PD) group (n = 312), Alzheimer’s disease (AD) group (n = 219), orother dementia group (n = 115) according to pathological features. The other dementia group comprised 25 patients with frontotemporal dementia, 38 with Lewy body dementia, 34 with vascular dementia, and 18 with semantic dementia. Serum vitamins (i.e., B1, B2, B3, B5, VB6, B9, and C) were measured via liquid chromatography-mass spectrometry/mass spectrometry. Total Hcy and plasma vitamin B12 and FA levels were measured using commercial electrochemiluminescence immunoassays.
Results: The serum levels of vitamins B1, B2, B5, B6, B9, and C were lower in all patient groups than in the control group. The logistic regression results revealed that lower levels of serum vitamins B2, B6, B9, and B12 were associated with a higher risk of dementia in PD patients, and higher Hcy levels and lower serum vitamin B6 and B9 levels were associated with a higher risk of AD-relatedcognitive impairment. In addition, the level of vitamins was positivelycorrelated with neuropsychological assessment scoresand negatively correlated with Hcy level and stage of dementia.
Conclusions: The levels of several water-soluble vitamins are lower in dementia patients. Moreover, lower levels of water-soluble vitamins and higher levels of Hcy may be associated with a higher risk of developing dementia. These findings suggest that estimating water-soluble vitamin levels in older adults may be valuable given that they may help improve cognitive function.
The association between school bullying involvement and Internet addiction among Chinese Southeastern adolescents: a moderated mediation model with depression and smoking
Authors: She Yuhang , Li Liping
Published: October 2024

Background
School bullying and Internet addiction are both common public health problems for adolescents. Several studies found an association between school bullying and Internet addiction; however, the underlying mediating and moderating mechanisms of the complex relationship between school bullying and Internet addiction are limited.
Objective
This study explored the mediating role of depression in the relationship between school bullying and Internet addiction and whether smoking moderated the relationship between school bullying and depression in Chinese Southeastern middle school students.
Methods
A cross-sectional study was conducted in Guangdong Province in Southeast China in June 2021. Associations between Internet addiction, school bullying, and depression were estimated using Spearman correlation analysis, the mediation effect and moderation effect were examined using Model 4 and Model 7 in the Hayes' PROCESS macro.
Results
The results included 1992 adolescents, 23.5% and 28.0% of participants reported experiences of school bullying and Internet addiction, respectively. There was a significant correlation between school bullying, depression, and internet addiction (p < 0.01). Depression partially mediated the association between school bullying and internet addiction, and smoking played a moderating role between school bullying and depression.
Conclusions
This study highlights the mediating and moderating mechanisms between school bullying and Internet addiction among Chinese Southeastern adolescents, providing the implication that depression and smoking interventions should be important in the prevention of Internet addiction among Chinese Southeastern adolescents.
A novel model of care; Telemedicine and peer support for HCV care among HIV infected people who inject drugs in remote Myanmar: A retrospective study
Authors: Tun Ni Ni , Smithuis Frank , Tun Nyan Lynn , Hteik Myo Min Min , Lynen Lutgarde , Conlon Christopher P. , Gils Tinne , Olmen Josefien van , Decroo Tom
Published: October 2024

Background: People who inject drugs (PWID) are at a heightened risk of co-infection with HIV and hepatitis C virus (HCV), which adversely affects health outcomes. Uncontrolled HCV can lead to increased transmission rates among PWID, highlighting the urgent need for improved access to treatment as a public health priority. Despite the availability of effective HCV treatments, access remains limited, particularly in remote areas, where stigma further complicates care. Implementing integrated and differentiated HCV care in these regions could help address this gap. This study evaluated a novel care model in remote settings, where general practitioners delivered integrated HCV-HIV care, telemonitored by specialists and supported by community health workers and peer educators. We evaluated treatment outcomes and associated predictors.
Methods: We used routine program data from the HCV treatment register to assess treatment completion rates and sustained virologic response (SVR) among PWID. SVR was defined as an undetectable HCV viral load 12 weeks after treatment completion. Patients who achieved SVR were invited for retesting at one year to calculate the one-year SVR rate. Logistic regression analyses were performed to identify predictors of both SVR and one-year SVR.
Results: Among 314 HIV-HCV co-infected PWID who initiated HCV treatment, 93.0% completed it, 96.2% had 12-week HCV-RNA results, and 77.9% achieved SVR. After one year, 67.7% (126 of 186) maintained SVR. Methadone maintenance therapy (aOR: 2.22; 95% CI: 1.09-4.55) and advanced liver disease—fibrosis (aOR: 2.33; 95% CI: 1.05-5.16) and cirrhosis (aOR: 3.21; 95% CI: 1.13-9.10)—were significantly linked to one-year SVR (p ≤ 0.05).
Conclusion: A novel care model involving general practitioners, specialist telemonitoring, and support from community actors has shown effectiveness for most HIV-HCV co-infected PWIDs. However, further qualitative research is needed to enhance SVR rates and better understand the reasons behind incomplete follow-up.
Metachronous small-bowel metastasis from pancreatic cancer presenting as anemia approximately 5 years postpancreatoduodenectomy: a case report
Authors: Tseng Ching-Wei , Tseng I-Tsou , Wu Chang-Che
Published: October 2024

Background: Intraductal papillary mucinous neoplasm (IPMN) is a premalignant cystic neoplasm originating from the pancreatic duct, with a risk of progression to pancreatic cancer. Surgical resection of IPMN has favorable outcomes, yielding high overall and disease-free survival rates. However, the incidence of malignant change or metastasis after surgical resection requiring repeat surgery remains high. Intestinal metastasis from pancreatic cancer, particularly after primary surgery, is extremely rare. Systemic treatments, including chemotherapy and radiotherapy, are typically used. Nevertheless, although surgery is not generally indicated, emerging research shows that it can be possibly beneficial for some patients
Case presentation: A 56-year-old female with a history of stage III nasopharyngeal cancer (T2N1M0), treated with concurrent chemoradiotherapy in 2012, was diagnosed with IPMN-associated pancreatic cancer (pT1cN0M0) in 2019. The tumor was located in the pancreatic head and the patient underwent a pancreaticoduodenectomy without adjuvant therapy. In early 2023, the patient developed chronic anemia(hemoglobin 8–9 g/dL) without gastrointestinal bleeding. Despite unremarkable endoscopic and computed tomography (CT) findings, a positron emission tomography-CT scan revealed a hypermetabolic lesion in the jejunum. Biopsy confirmed adenocarcinoma of pancreatic origin, consistent with metastasis. In January 2024, re-resection of the jejunal metastasis and biliary reconstruction were performed. The patient recovered uneventfully, and pathology confirmed pancreatic adenocarcinoma metastasis.
Conclusion: This case presents a rare occurrence of jejunal metastasis following pancreaticoduodenectomy for IPMN-associated pancreatic cancer. Successful re-resection of the metastatic lesion highlights the potential role of surgery in select cases of oligometastatic recurrence. While chemotherapy remains the standard treatment for metastatic pancreatic cancer, surgical intervention should be considered in patients with limited recurrence, particularly when complete tumor resection is feasible. Regular surveillance with imaging and tumor markers is essential for early detection of recurrence and improving long-term outcomes.
Cascade upconversion: a strategy enabling four-photon lithography in week light
Authors: Gan Zongsong , Li Shishuo , Li Kai , Yi Chenqi
Published: October 2024

Multiphoton lithography(MPL) offers the highest resolution available in submicron scale true 3D printing, but excessive femtosecond laser intensity prevents it from leading to higher photon counts. To circumvent away this effect, we present a cascade upconversion (CUC) strategy, which is a combination of two efficient two-photon upconversion processes to achieve four-photon photopolymerization. In order to demonstrate the advantages and feasibility of this approach, we combine excited state absorption upconversion using high concentration Ho3+/Yb3+ doped upconversion nanoparticles (UCNPs) with triplet-triplet annihilation (TTA) upconversion to fabricate 3D polymer structures by low-cost continuous wave 980 nm laser at 105 W/cm2. This method overcomes the diffusivity caused by isotropy of UCNPs luminescence, and achieves two orders of magnitude improvement in resolution while maintaining the advantages of using near infrared(NIR) light. This new strategy provides a general way for designing four-photon and even six-photon MPL in week light.
Microbial analyses of infectious keloids on the anterior chest— a case-control study
Authors: Chen Qian , Hou Shen , Wu Xiao-Yan , Bu Wen-Bo , Zhou Bing-Rong , Chen Xiao-Dong
Published: October 2024

Backgroud:
Some studies confirmed the presence of pathogen may cause infection by bacterial cultures on the surface of infectious keloids. But further exploration of comparison with non-infectious keloids and the bacterial flora of infectious foci was lacking.
Objective
To investigate the differential flora of purulent secretions on the surface of infectious keloids compared to the surface of non-infectious keloids. And to determine the microbial composition within the infectious foci.
Methods
This case-control study of 17 patients obtained swab specimens from the surface of keloids from two groups and the infectious foci from the infectious group. Bacterial composition was analyzed by 16S ribosomal RNA sequencing.
Results
There was no statistical difference in the general condition of patients from two groups. The number of the phylum Actinobacteriota, the order Propionibacteriales, Corynebacteriales, and the genus Taibaiella was more on the keloids surface in the infectious group. The three most represented genera in infective sites were the genus Staphylococcus, Peptoniphilus, and Cutibacterium. Microbial-associated gene pathways revealed a correlation with inflammation and tumor like growth of keloids.
Conclusion
There was a link between infectious keloids and microorganisms, providing direction for the prediction and treatment of keloid infections.
A Rare Case of Adult-Onset Vanishing White Matter Leukoencephalopathy With Movement Disorder
Authors: Douden Bashar Kamal Ali , Abufara Yazan Mohammad Abdullah , Aldrabeeh Mahmood Fayez Ali , Tell Naela Ramadan Mohammad , Abudaya Ismail
Published: October 2024

Background:
Vanishing white matter disease (VWMD) is a rare autosomal recessive leukoencephalopathy. It is typified by a gradual loss of white matter in the brain and spinal cord, which results in impairments in vision and hearing, cerebellar ataxia, muscular weakness, stiffness, seizures, and dysarthria cogitative decline. The majority of reports involve minors. Very few instances worldwide have been reported, with adult onset of vanishing white matter considered to account for 15% of cases. Clinical evaluation, MRI results, and confirmatory genetic testing are used to diagnose VWMD.
Case presentation:
A 39-year-old male from Hebron, Palestine, presented with a 7-month history of postural instability, imbalanced gait, and progressive deterioration of his lower extremities. Additionally, the patient suffered from ocular abnormalities and sphincteric issues. The patient's sibling showed comparable symptoms but was never diagnosed, as he passed away as a result of colon cancer, as per his family history. Reduced cognitive function, spastic quadriparesis, hyperreflexia, bradykinesia, and shuffling gait were found during a neurological examination. Normal results were obtained from routine laboratory tests, including cerebrospinal fluid (CSF), blood, and urine. Periventricular white matter hyperintensities, which are indicative of vanishing white matter leukoencephalopathy (VWML), were identified during an MRI. The diagnosis of adult-onset VWML with movement disability was substantiated by genetic testing, which named a homozygous pathogenic missense mutation, EIF2B3, and a deletion in PRKN/PARK2. The patient's motor symptoms were temporarily alleviated following the administration of Levodopa/Carbidopa. Nevertheless, the long-term consequences are uncertain due to the illness's ongoing progression and the absence of a cure at this time.
Conclusion:
This instance of vanishing white matter leukoencephalopathy (VWML) is particularly remarkable in adults because of its rarity and complexity. The diagnosis is further complicated by the coexistence of Parkinsonism and VWML. Although a cure is not currently known. Early discovery is crucial to effectively manage symptoms. This example underscores the importance of more VWML research, particularly in Palestine, where studies on neurological disorders are limited. These findings underscore the importance of enhancing the region's diagnostic and therapeutic capabilities.
Tooth loss related risk factors and oral microbiota changes in older adults: a cross-sectional study
Authors: Zhang Chenze , Jiang Jiapei , Huang Lirong , Cheng Xingqun , Wu Hongkun
Published: October 2024

Objectives
This study aimed to analyze the risk factors of tooth loss in the elderly, and to compare the structure and composition of the salivary microbiota between the low residual tooth group and the high residual tooth group, providing theoretical basis for the development of professional and effective preventive and management strategies to improve tooth loss in the elderly.
Materials and methods
A sexual cross-sectional study was conducted in 310 elderly people over 60 years old. Through clinical examination and questionnaire survey, the risk factors of tooth loss were collected. Monomial and polynomial logistic regression models were used to estimate the data. Non-irritant saliva samples were collected from people in low residual tooth group and high residual tooth group (n = 25/each group) for 16S rRNA sequencing analysis.
Results
310 elderly people were categorized into two groups based on their residual teeth count: low residual teeth group (n = 87) and high residual teeth group (n = 223). Tooth loss in the elderly is closely related to age, root caries, frequency of eating dessert and candies, toothpick use, self-rated oral health, and frailty according to the multifactorial analysis. In terms of microbial composition, the proportion of Porphyromonas, Treponema, Asticcacaulis, Filifactor and Prevotella in the low residual tooth group were significantly lower than that in the high residual tooth group.
Conclusion
Tooth loss is closely related to the aging, poor living habits, and poor oral and physical conditions of the elderly. The proportion of pathogenic bacteria in the elderly population with more tooth is higher.
Clinical relevance
This study provides some risk factors that can be directly assessed through clinical examination and chairside questionnaires, and provides guidance for subsequent laboratory testing of the microbiota composition of patients' saliva, which is conducive to timely knowledge popularization and long-term help for elderly patients.
YTHDF2 Promotes the Metastasis of Oral Squamous Cell Carcinoma through the JAK- STAT Pathway
Authors: chen zhezheng , Zhao Dan , yuan yamin , Zeng Lu , Luo Zhengzhou , Chen Junliang , Lan Xiaorong , He Yun , Liu Lin
Published: October 2024

Background: RNA-binding proteins act as crucial mediators between m6A modification and RNA function, playing a significant role in the recurrence and metastasis of oral squamous cell carcinoma. YTHDF2, the first identified RNA-binding protein, is associated with the prognosis of certain cancer patients. However, the role of YTHDF2 in oral squamous cell carcinoma and its underlying molecular mechanism have not been fully explored.
Methods: First, we analysed the expression levels of YTHDF2 and itscorrelation with clinical features via the use of public databases and oral squamous cell carcinoma patient samples. Subsequently, in vitro functional experiments were conducted to assess the effects of YTHDF2 on the proliferation, migration and invasion capabilities of oral squamous cell carcinoma cells. Additionally, RNA-seq analysis was utilized to investigate the signalling pathways modulated by YTHDF2, followed by experimental validation.
Results: Our findings revealed that YTHDF2 expression was significantly elevated in oral squamous cell carcinoma tissues and cells, with levels significantly correlated with the clinical stage, pathological grade, and survival time of patients. The knockdown of YTHDF2 resulted in decreased proliferation, migration and invasion abilities in oral squamous cell carcinoma cells. Furthermore, RNA sequencing data indicated that silencing YTHDF2 suppressed the JAK-STAT signalling pathway, and the use of STAT3 activators reversed this suppressive effect in oral squamous cell carcinoma cells.
Conclusion: Our study demonstrated that YTHDF2 promotes the proliferation, metastasis and invasion of oral squamous cell carcinoma by positively regulating the JAK-STAT signalling pathway, suggesting that YTHDF2 could serve as a potential prognostic marker for oral squamous cell carcinoma.
Demographic, clinical, treatment, outcome, and comorbidities of patients with relapsing polychondritis: Experience from Brazilian tertiary center, and literature review
Authors: Ueda Patricia Pilar Lury Ortale , Komati Luiz Antonio Leandrini , Shinjo Samuel Katsuyuki
Published: October 2024

Background Due to the rarity of relapsing polychondritis (RP), we described the demographic, clinical, treatment, outcomes, and comorbidities of patients with RP from our tertiary service. Additionally, a literature review was conducted.Methods A total of 47 Brazilian patients with RP between 2000 and 2024 were analyzed. All patient data were collected from pre-parametrized and pre-standardized electronic medical records. A literature review using PubMed with “relapsing polychondritis” as the search term included 25 articles after applying the strict exclusion criteria.Results A total of 47 patients were evaluated. The median age was 40 (34–51) years, with a female-to-male ratio of 1.4:1, and 89.4% were of white ethnicity. The median time from symptom onset to diagnosis was 39 months and the median follow-up duration was 7 years. Ear cartilage biopsy was performed in 12.8% of cases. The clinical manifestations included auricular chondritis, arthralgia, and ocular involvement. Approximately half of the patients had hypertension and dyslipidemia, one-third had diabetes mellitus, and one-fifth had hypothyroidism. Tracheostomy and cochlear implantation were required in 12.8% and 6.4% of the patients, respectively. Disease outcomes showed that 46.8% of patients were in remission, 29.8% had active disease, and 25.5% were controlled with immunosuppressive therapy. Mortality occurred in 4.3% of the cases. In the literature review, 25 studies were analyzed, most of which originated in Asia. Studies have reported the classical manifestations of RP, such as auricular chondritis, arthritis, and ocular involvement. The median age of the patients with RP was similar across studies, averaging 46.4 years, with a predominance of female patients. A comparison with the literature showed consistency in clinical manifestations, particularly auricular chondritis and septum nasal chondritis, although few studies have explored comorbidities, disease evolution, and outcomes.Conclusions The reviewed articles described classical clinical manifestations, but few articles reported data on other manifestations and comorbidities that can occur in RP. Our study provided new insights by mapping symptom evaluations, thereby enhancing the understanding of disease evolution. Understanding and characterizing RP will allow for better assistance in its diagnosis and follow-up.
Review of Intrauterine Insemination in Polycystic Ovarian Syndromes - Cases Review and Current Approach
Authors: Suryawan Alfonsus Zeus , Susilo Artha Falentin Putri , Djuwantono Fridya Wulandari
Published: October 2024

Intrauterine insemination (IUI) is procedure which we introduce sperm into the fundal cavity. It’s help a lot of infertile couple to reach clinical pregnancy and currently mainly first choice of unexplained fertility. Usage in other cause of infertility was also consider with individualized approach. However IUI is not without flaw, it’s success rate rely heavily on sperm count and motility. It’s usage in polycystic ovary syndrome (PCOS) still in debates, due to high insulin resistance condition in PCOS hinders implantation and increase risk of fetal loss associated with endometrial dysfunction. This serial case report shows want to review 4 cases of IUI with PCOS on Aster Fertility Clinic from September 2023 – July 2024 and current approach of this condition which relevant to nowadays practice and knowledge.
The effect of mastoid vibration on joint angles of lower extremities during walking: implication for sensorimotor training for future spaceflight
Authors: Xie Haoyu , Wang Zhuo , Wang Chuhuai , Chien Jung Hung
Published: October 2024

A spaceflight in microgravity unloads the peripheral vestibular organs and disturbs the vestibular system of astronauts. This disruption of the vestibular system forces astronauts to use different strategies to walk, such as flexing more on their knee joints to increase force to propel their bodies forward on the Moon. It should be noted that this vestibular disruption observed in microgravity cannot easily be replicated on Earth. However, mastoid vibration (MV) has been shown to be an effective alternative method for disrupting vestibular function. The application of MV during walking has been demonstrated to alter gait characteristics regardless of the different terrains. Could these changes in gait characteristics induced by MV also affect joint angle patterns in a similar manner? Therefore, the purpose of this study was to fill this knowledge gap. This study involved fifteen young adults and ten older adults. Two motion capture cylinders were used to record the trajectory of 10 body land markers. Two electromechanical vibrotactile transducers were placed on bilateral mastoid processes to generate the MV at 100Hz. A reduced-light intensity goggle was used to reduce the visual capability during walking. A total of six 2-minute walking trials were randomly assigned to each participant (3 MV: bilateral/unilateral/no MV x 2 vision levels: full/reduced vision). Dependent variables included peak values, range of motion (ROM), and respective hip, knee, and ankle joint variabilities. A significant interaction between the effects of aging, vision level, and different types of MV was observed in the peak hip flexion angle (p = 0.001). Post hoc comparisons indicated that 1) applying bilateral MV significantly decreased peak hip flexion of healthy young but increased peak hip flexion of healthy young in reduced vision condition, and 2) applying bilateral MV induced a greater effect on decreasing hip flexion than applying unilateral MV in all participants regardless of whether vision was full or reduced. The findings of this study revealed the important role of hip joints in balance control during gait under vestibular and/or visual perturbation. Also, MV could be used to simulate a vestibular-disrupted environment for astronauts for future sensorimotor training.
Bionic fractionalization in the trimer model of twisted bilayer graphene
Authors: Kim Eun-Ah , Zhang Kevin , Mao Dan , Moessner Roderich
Published: October 2024

Motivated by the rapid experimental progress in twisted van der Waals materials, we study the triangular trimer model as a representative framework for extended Wannier orbitals in twisted bilayer graphene at 1/3-filling. This deceptively simple model exhibits a rich suite of complex phases, including unusual excitations exhibiting the physics of fractionalization and fractons. For our investigations, we carry out extensive Monte Carlo simulations using an efficient cluster algorithm. The so-obtained finite-temperature phase diagram reveals a novel polar fluid and an ordered brick-wall phase characterized by fractionally charged e/3 excitations with subdimensional lineonic dynamics. Notably, we identify a critical trimer liquid phase for the particularly simple model of hard trimers. For this, we derive a new field theory which takes the form of a U(1)×U(1) gauge theory. Its e/3 monomers are fractionalized bionic excitations: they carry a pair of emergent gauge charges, as evidenced by algebraic correlations with two distinct exponents. These field theoretical predictions offer theoretical grounds for numerical observations of critical exponents. Our study highlights the triangular trimer model as a new key platform for investigating fractionalization and fractons, where trimer liquid bionic monomers can transform into lineons or fractons in proximate phases, and calls for experimental investigations of this physics in twisted van der Waals materials and a broader class of systems with intermediate-range interactions.
Awareness towards obstetric fistula and associated factors among reproductive age group women in Bibugn district, East Gojjam, Amhara, Northwest Ethiopia; Cross sectional study
Authors: Kassahun Bezawit , Degu Genet , Talie Asmare , Chanyalew Lemlem
Published: October 2024

Background
Obstetric fistula is a hole which develops between female private organs and leaves a woman incontinent of urine or faeces or both. It is a devastating public health problem usually caused by obstructed labour. Its occurrence is correlated with lack of awareness. The key to eliminate obstetric fistula is raising awareness of it and the factors that contribute to it.
Objective
To assess awareness towards obstetric fistula and associated factors among reproductive age women in Bibugn district, East Gojjam zone, Amhara, Ethiopia, 2022.
Methods
A community based cross-sectional study was employed to collect data from 612 reproductive age women from May20-June20/2022. A systematic sampling technique was used to select study participants. Data were collected using a pretested structured face to face interviewer- administered questionnaire. Bivariable and multivariable analysis were used to identify factors associated with the dependant variable.
Result
From total sampled participants, of them 608(99.3%) were interviewed, from those 37.8%[95%CI (34, 42%)]study participants had good awareness towards obstetrics fistula. Primary andsecondaryeducation(AOR = 4.91,CI = 3.10,7.99), and (AOR = 6.37, CI = 3.70,10.91) respectively, being urban resident (AOR = 1.71,CI = 1.09,2.67), contraceptive use (AOR = 1.70,CI = 1.04, 2.79), and distance < 30minute on foot from health facility(AOR = 0.42,CI = 0.34,0.79) weresignificantly associated with awareness towards obstetric fistula.
Conclusion and Recommendation
This study reported that awareness of obstetrics fistula was low compared to previous studies in Ethiopia. Urban residency, education (primary and above), having a history of using contraceptives and proximity to a health facility were significantly associated with awareness towards obstetric fistula. Measures to improve educational level and contraceptive utilization of reproductive age women should be considered.
High Ano1 expression as key driver of resistance to radiation and cisplatin in HPV-negative head and neck squamous cell carcinoma
Authors: Bourdier Solenne , Fisch Anne-Sophie , Alp Keziban Merve , Das Ridhima , Mertins Philipp , Tinhofer Ingeborg
Published: October 2024

Human papilloma virus-negative head and neck squamous cell carcinoma (HNSCC) frequently harbors 11q13 amplifications. Among the oncogenes at this locus, CCND1 and ANO1 are linked to poor prognosis; however, their individual roles in treatment resistance remain unclear. The impact of Cyclin D1 and Ano1 overexpression on survival was analyzed using the TCGA HNSCC dataset and a Charité cohort treated with cisplatin (CDDP)-based radiochemotherapy. High Ano1 expression was primarily associated with poor overall survival in both datasets. The effects of CCND1 and ANO1 knockdown (KD) on radio- and drug sensitivity, along with changes in global protein expression, cell viability, growth, and DNA repair, were studied in an 11q13-amplified HNSCC cell line model of primary cisplatin resistance. Unique pathway alterations– VEGF in CCND1 KD and the Rho GTPase cycle in ANO1 KD– were observed, along with shared changes like DNA damage and cell cycle dysregulation. Silencing Cyclin D1 or Ano1 increased CDDP sensitivity, while only Ano1 silencing increased radiosensitivity. Copanlisib and afatinib were identified as promising candidates for combination therapy of 11q13-amplified HNSCC tumors. We demonstrated a predominant role for Ano1 in treatment resistance in Cyclin D1highAno1high HNSCC tumors and identified novel potential treatment combinations for this high-risk patient group.