bluuebunny/crossref_metadata_2025 huggingface dataset:
Sosialisasi Silase Keong Mas Sebagai Sumber Protein Pakan Di Kecamatan Sorkam
Authors: Sinaga Daniel
Published: March 2024

Salah satu faktor yang berperan besar dalam pemeliharaan ternak unggas yaitu pakan, selain itu salah satu sumber pengeluaran terbesar didalam pemeliharaan ternak unggas yaitu pakan yang berbahan baku tepung ikan yang memiliki harga cukup tinggi dikarenakan tepung ikan merupakan produk impor. Untuk mengurangi penggunaan tepung ikan tersebut maka digunakanlah bahan baku lokal yang mudah didapat dan memiliki kandungan nutrient yang tinggi yaitu Keong mas. Pengabdian kepada masyarakat ini dilakukan dalam bentuk sosialiasi bahan baku lokal silase Keong mas sebagian sumber protein untuk ternak unggas di Kecamatan Sorkam Kabupaten Tapanuli Tengah Provinsi Sumatera Utara. Kegiatan pengabdian ini  dilakukan pada tanggal 16 September 2020 yang berlokasi di Aula Kecamatan Sorkam, Kabupaten Tapanuli Tengah, Provinsi Sumatera Utara. Tahapan kegiatan ini dimulai dari kegiatan survei lokasi yang akan digunakan untuk acara pelaksanaan, Sosialisasi bahan baku silase Keong mas dan Evaluasi hasil dari sosialiasi yang telah dilaksanakan. Hasil kegiatan yang didapatkan dari sosialisasi ini yaitu meningkatkan pengetahuan dan keterampilan peserta tentang penggunaan bahan baku lokal silase Keong mas sebagai sumber protein untuk pakan ternak unggas. Adapun materi yang dijelaskan antara lain 1) proses pembuatan silase Keong mas, 2) manfaat silase Keong mas pada ternak unggas 3) formulasi pakan untuk pembuatan pakan ternak unggas.
Peningkatan Pengetahuan Masyarakat Terhadap Manajemen Risiko Banjir di Martapura, Kabupaten Banjar
Authors: Yuliana Candra , Sofia Elma , Widiastuti Endah , Barkiah Ida
Published: March 2024

Perubahan iklim dipengaruhi oleh kondisi cuaca yang tidak stabil sebagai contoh curah hujan yang tidak menentu, sering terjadi badai, suhu cuaca yang ekstrim, dan arah angin yang berubah drastis hingga akhirnya menyebabkan banjir atau tanah longsor. Permasalahan yang mendasar adalah kurangnya kesiapsiagaan atas bencana banjir yang terjadi di daerah Martapura, Kabupaten Banjar. Manajemen risiko merupakan kegiatan mendasar yang diarahkan untuk mengevaluasi skema untuk mengurangi risiko tetapi tidak untuk menghilangkan semua risiko, karena risiko dalam banyak kasus tidak dapat sepenuhnya dihilangkan. Pada pengabdian ini akan memberikan informasi mengenai risiko banjir dan genangan secara terintegrasi dengan memberikan analisis terhadap kesiapsiagaan terhadap kejadian banjir oleh masyarakat. Sehingga dapat memberikan referensi terkait mitigasi bencana banjir bagi SMPN 5 Martapura khususnya serta masyarakat Martapura pada umumnya.
Pengembangan Program Madrasah Berbasis Riset Stem Potensi Lokal di Lereng Gunung Muria
Authors: Malichatin Hanik , Prasetyo Dody Rahayu
Published: March 2024

Sebagai upaya menanamkan kecintaan siswa madrasah terhadap riset dan optimalisasi pemanfaatan potensi lokal di lereng gunung Muria dapat dilakukan dengan cara mengembangkan madrasah berbasis riset. Melalui program madrasah berbasis riset STEM (Science, Technology, Engineering, Mathematic), diharapkan masyarakat di sekitar madrasah lebih memahami cara mengoptimalkan potensi lokal tersebut. Sampel pengabdian yang dipilih yaitu Madrasah Tsanawiyah (MTs) NU Raden Umar Said yang berlokasi di lereng gunung muria. Metode pengabdian dilakukan dengan tiga tahap yaitu tahap persiapan, pelaksanaan, dan evaluasi. Hasil pengabdian yang diperoleh 1) limbah kulit jeruk pamelo dapat dijadikan bahan dasar pembuatan sabun dan biobaterai, 2) para siswa antusias dalam pembelajaran riset STEM, tetapi keterampilan menggunakan alat praktikum masih rendah, dan 3) madrasah membuat program ekstrakurikuler riset STEM berbasis potensi lokal di gunung Muria. Melalui kegiatan ini, MTs NU Raden Umar Saâ€™id dapat menjadi pioner madrasah riset di gunung Muria. Dengan demikian, diharapkan dapat mencontoh madrasah di sekitarnya.Kata kunci: Madrasah berbasis Riset STEM, potensi lokal
Pendampingan Pembuatan Media Pembelajaran Berbasis Multimedia Bagi Guru SD Negeri Pedurungan Kidul 02 Semarang
Authors: Utomo Danang Wahyu , Kartikadarma Etika , Dolphina Erlin , Kurniawan Defri , Purwanto Purwanto
Published: March 2024

Media pembelajaran saat ini telah berkembang, salah satu contohnya adalah media pembelajaran digital atau biasa disebut literasi digital. Literasi digital dapat berupa teks, audio, atau video. Cara mendapatkannya dapat melalui berbagai sumber seperti media sosial dan halaman web. Keuntungan dari media pembelajaran digital adalah dapat meningkatkan kemampuan belajar siswa. Guru juga dapat menggunakan berbagai sumber seperti teks, gambar, audio dan video dalam materi pembelajaran. Maka program kemitraan Masyarakat (PKM) dari Udinus menawarkan pendampingan pembuatan media pembelajaran berbasis multimedia dengan Canva. Metode yang digunakan dalam program kemitraan Masyarakat adalah praktek dengan Canva. Dalam praktek tersebut, para guru diawali membuat slide presentasi kemudian diubah menjadi video pembelajaran dengan memanfaatkan asset yang disediakan oleh Canva.
Pemanfaatan Desain Poster Sebagai Sarana Publikasi Branding Kedai Es Koffie Cap Lyon
Authors: Alfiyan Faruk , Novandi Aditya Rizky
Published: March 2024

Kedai Es Koffie Cap Lyon adalah salah satu usaha kuliner yang berada di kawasan Pecinan Kabupaten Banyuwangi. Kedai yang disetting sebagai tempat berkumpulnya anak muda ini memiliki nuansa Chinnese Town yang instagrammable. Namun demikian, kedai ini masih harus melakukan penguatan branding dan melakukan publikasi secara all out agar dapat lebih dikenal oleh masyarakat secara luas, khususnya generasi milenial. Hal ini dikarenakan saat ini telah banyak bermunculan usaha-usaha sejenis yang menjadi kompetitornya. Untuk mengatasi permasalahan tersebut, maka perlu adanya inovasi kreatif yang dapat membantu dan memaksimalkan publikasi yang dilakukan oleh pihak kedai. Dengan memanfaatkan desain poster yang menarik pada interior ruang dan seluruh aktifitas yang dilakukan, publikasi yang dilakukan akan dapat lebih maksimal. Hasil dari kegiatan pengabdian masyarakat ini adalah dibuatnya poster branding kedai yang lebih artistik pada semua kemasan produk, layanan kedai, promo, discount, hingga untuk kepentingan interior ruang dan posting di media sosial. Sedangkan tujuan yang ingin dicapai adalah membantu pihak mitra menghasilkan desain poster yang berkualitas dan layak untuk dipublikasikan di berbagai media yang ada, sehingga branding Kedai Es Koffie Cap Lyon akan tetap dikenal oleh masyarakat secara luas dan dapat bersaing dengan para kompetitornya.
Pelatihan 3D Model dan Pemahaman Arsitektur Nusantara Pada Tenaga Pengajar SMK St. Louis Dengan SketchuUp
Authors: Trisjanti Lucia Ina , Putra Heristama Anugerah , Hastorahmanto Prabani Setio , Roosandriantini Josephine
Published: March 2024

Pembelajaran semakin berkembang pada sekolah SMK, karena untuk mempersiapkan siswa – siswi yang siap pakai di dunia kerja. Oleh karena itu guru pada sekolah SMK St. Louis ingin mengembangkan kompetensi dalam penguasaan program sketchup dalam menghasilkan produk 3D model. Penguasaan kompetensi program sketchup ini dapat diterapkan pada saat pembelajaran siswa-siswi di jurusan DKV. Pengabdian ini dijalankan melalui sebuah pelatihan secara offline yang diikuti oleh guru-guru pengajar SMK St. Louis di Surabaya. Pembahasan menunjukkan bahwa kompetensi penguasaan para guru SMK St. Louis yaitu menghasilkan 3D model menggunakan blender. Hal itu telah menyebabkan munculnya pemikiran bahwa untuk lebih mempermudah dalam menghasilkan 3D model yaitu menggunakan sketchup. Pemahaman mengenai Arsitektur Nusantara merupakan topik yang dapat dikembangkan oleh guru SMK St. Louis, dalam pengaplikasiannya dalam produk atau karya desain. Oleh karena ide itu maka dalam pelatihan 3D model ditambahkan penjelasan mengenai arsitektur Nusantara. Pelatihan yang diikuti oleh guru SMK St. Louis menyatakan menjadi memiliki pengetahuan atau alternatif materi dalam pembelajaran pada siswa – siswi SMK St. Louis, saat menghasilkan 3D model dengan sketchup dan Arsitektur Nusantara.
Peningkatan Semangat Kebangsaan Masyarakat bersama Lembaga Bimbingan Belajar (LBB) Delta Fermion
Authors: Tamimah Ni'matut , Siregar Aslam Chitami Priawan
Published: March 2024

Banyaknya pengaruh asing yang berpotensi dapat merusak keutuhan Bangsa Indonesia. Oleh karena itu, dengan adanya peringatan hari kemerdekaan 17 Agustus dapat menjadi momen yang sangat tepat bagi warga negara Indonesia untuk meningkatkan kembali semangat kebangsaan. Bekerjasama dengan LBB Delta Fermion Sidoarjo, maka dibuatkan suatu kegiatan yang dapat meningkatkan semangat kebangsaan bagi warga sekitar di Dusun Klutuk Desa Kramat Jegu Kecamatan Taman Kabupaten Sidoarjo. Beberapa acara yang dilakukan dalam kegiatan tersebut antara lain, yaitu: Pertama, pemberian pendidikan motivasi dengan cara mengenang jasa para pahlawan agar dapat meneruskan cita-cita mereka dalam menjaga keutuhan Negara Kesatuan Republik Indonesia (NKRI). Kedua, pelatihan pendidikan kesehatan jasmani seperti pada senam pagi dan jalan sehat agar dapat menjaga keseimbangan fisik masyarakat sebagai penerus generasi bangsa yang kuat. Ketiga, pemberian hadiah kepada masyarakat sekitar agar dapat meningkatkan kepedulian kepada sesama masyarakat Indonesia. Dengan adanya acara tersebut, diharapkan semangat kebangsaan dan kepedulian kepada sesama dapat meningkat sehingga tercipta masyarakat Indonesia yang rukun, aman, dan damai.
Pendampingan Pelaporan SPT Tahunan Orang Pribadi Karyawan di Kabupaten Kutai Kartanegara
Authors: Febriani Eka
Published: March 2024

Pendampingan ini memiliki tujuan untuk meningkatkan pemahaman pengetahuan mitra kegiatan ini akan perpajakan dan pemenuhan kewajiban peserta yang dalam kegiatan ini adalah wajib pajak orang pribadi karyawan dalam pelaporan Surat Pemberitahuan (SPT) Tahunan. Pendampingan ini dilaksanakan pada Unit Mikro sebuah BUMN di Kabupaten Kutai Kartanegara cabang Tenggarong dan Timbau. Kegiatan pendampingan ini dilaksanakan dengan metode pendampingan langsung secara one on one dengan wajib pajak orang pribadi karyawan dalam pelaporan SPT Tahunan. Selama kegiatan, ditemukan bahwa faktor penting yang sangat perlu diperhatikan olah wajib pajak orang pribadi karyawam dalam melakukan pelaporan SPT Tahunan adalah melakukan penyimpanan data terkait kewajiban perpajakan secara rapi seperti pin akun, Efin, email dan nomor handphone yang didaftarkan, agar pelaporan SPT di tahun-tahun berikutnya dapat dilaksanakan dengan lancar. Selain itu, wajib pajak orang pribadi karyawan memerlukan pemahaman yang lebih mendalam mengenai skema perhitungan Pajak Penghasilan Pasal 21 agar ketika terjadi kesalahan pembuatan bukti potong dari pihak pemotong, maka Wajib Pajak Orang Pribadi Karyawan dapat mengetahui dan meminta perbaikan kepada pihak pemotong pajak.
Optimasi Investasi di Pasar Saham Indonesia: Meningkatkan Keputusan Investasi dengan Prediksi IHSG menggunakan Decision Tree
Authors: Waluyo Dwi Eko , Paramita Cinantya , Kinasih Hayu Wikan , Rafrastara Fauzi Adi , Pergiwati Dewi
Published: March 2024

Pasar saham Indonesia merupakan pilar ekonomi yang vital, memfasilitasi perolehan modal bagi perusahaan serta menawarkan peluang investasi bagi individu hingga korporasi besar. Keberhasilan investasi sangat dipengaruhi oleh kemampuan memahami faktor-faktor yang menentukan pergerakan harga saham. Teknologi dan analisis data, khususnya melalui algoritma Decision Tree, dapat membantu memprediksi pergerakan Indeks Harga Saham Gabungan (IHSG), sehingga mendukung keputusan investasi yang lebih baik. Pengabdian masyarakat bertajuk "Optimasi Investasi di Pasar Saham Indonesia" dirancang untuk meningkatkan literasi investasi di kalangan mahasiswa, pengusaha dan pemegang saham, melalui pengembangan system analisis berbasis Decision Tree untuk prediksi IHSG. Program ini mencakup penelitian awal, pengembangan dan validasi model prediksi, pelatihan dan edukasi, implementasi, serta evaluasi dan penyempurnaan berbasis MOS, dengan tujuan akhir meningkatkan keberhasilan investasi di pasar saham Indonesia, seraya mengintegrasikan pengetahuan di bidang komputer, AI, dan keuangan. Materi pelatihan mencakup dasar analisis teknikal dan fundamental, analisis Decision Tree, optimasi portofolio, dan strategi manajemen risiko, dilengkapi dengan alat machine learning. Evaluasi pasca pelatihan menggunakan metode Mean Opinion Score (MOS) menunjukkan tingkat kepuasan tinggi dengan skor 97.08% untuk Fungsionalitas, 96.09% untuk Keandalan, dan 98.09% untuk Kegunaan, menekankan efektivitas algoritma Decision Tree dalam memprediksi IHSG dan meningkatkan keputusan investasi.
Edukasi Perilaku Hidup Bersih dan Sehat di Sekolah Dasar, Kepulauan Selayar
Authors: Ningsih Andi Pramesti , Makkau Bukroanah Amir , Indirwan Dicky
Published: March 2024

Sikap dan tindakan yang dilakukan oleh individu dengan tujuan menjaga kesehatan dikenal sebagai perilaku hidup bersih dan sehat (PHBS). Perilaku ini penting dalam rangka mencegah terjadinya penyakit. Permasalahan perilaku yang sering terjadi pada anak SD berkaitan dengan kebersihan gigi, tangan, rambut dan badan. Perilaku tidak sehat ini dapat mengakibatkan penyakit seperti sakit gigi, diare dan demam. Pengabdian masyarakat ini diawali dengan perizinan sekolah dan identifikasi kegiatan untuk mengetahui apakah sebelumnya sudah pernah diadakan penyuluhan dengan topik yang sama. Dari proses tersebut, diketahui bahwa penyuluhan dengan tema perilaku hidup bersih dan sehat belum pernah dilakukan. Adapun tujuan dari pengabdian masyarakat dalam bentuk penyuluhan ini adalah untuk memberikan edukasi kepada anak sekolah dasar mengenai cara untuk menjaga kebersihan diri sehingga terhindar dari penyakit dan memiliki rasa nyaman dalam beraktivitas.
Pelatihan Pembuatan Digital Brosur Promosi UMKM di Kabupaten Banyumas
Authors: Purwandari Gigih Ariastuti , Puspitasari Indah , Setiawan Kristianto , Puspita Sari Eka Dyah , Wijayanti Lely Tri
Published: March 2024

AbstractThe community service is aimed to provide knowledge about brochure visualization. The method used to achieve these objectives involves activities such as presentations, training, and mentoring, which include knowledge building, modeling, joint construction, and independent construction activities. To measure the success of the activity, there are three types of evaluation: (1) the initial stage through a Pre-test. Questions are posed through a Google form to assess the participants' initial knowledge, (2) the implementation stage/process of the activity through observation, discussion, practice, and assessment of the final products, and (3) the final stage through a post-test, where answers are assessed to determine the knowledge improvement after participating in this PkM. With these evaluation stages, the knowledge and skills of the participants are assessed to understand their improved understanding of brochure visualization knowledge and skills. As a result, there is an increase in knowledge about brochure visualization. Through this activity, issues related to optimizing knowledge and skills in brochure visualization can be addressed.
La interculturalidad en la educación básica de Ecuador
Authors: Espinoza Freire Eudaldo Enrique
Published: March 2024

The present work is the result of a descriptive investigation of type bibliographic revision with qualitative approach. Methods of theoretical cut were employed, such as analytical-synthetic and hermeneutic, as well as the technique of content analysis, which allowed to determine that Ecuador has a solid legal framework based on the Constitution of the Republic of 2008, which recognizes the multicultural character and plurinational of its population; Intercultural Education is a process of cognitive, procedural and attitudinal construction of relations between cultures; that can not be reduced to classroom and curricular contexts; it is also a set of political, social and cultural processes, which requires pedagogical models that are specified in policies, programs and projects to generate learning environments characterized by cultural interaction; where all the cultural features must be taken into account to educate the young generations in a coexistence of respect, solidarity, equity, flexibility and tolerance; to live in an increasingly complex society, which undergoes new sociocultural changes, generating the expansion of the links between diverse cultural groups; This is precisely the essence of the importance of intercultural education in Ecuadorian basic education.
Inovasi Pembelajaran Bahasa Baku Dengan Media Pembelajaran Game Edukasi Who Wants To Be A Millionaire
Authors: Kusnasari Zanida Zulfana , Rakhmawati Ani
Published: March 2024

Guru dapat menggabungkan teknologi, seperti game edukasi yang berkembang pesat dan menarik perhatian siswa ke dalam pembelajaran di kelas. Tujuan pemanfaatan media pembelajaran game edukasi dalam pendidikan adalah untuk membuat pembelajaran lebih menarik, aktif, dan efektif, sekaligus meningkatkan pemahaman siswa mengenai pembelajaran bahasa. Penelitian ini bermaksud untuk mengetahui dan membuktikan adanya perbedaan nilai akhir dan hasil belajar mata pelajaran bahasa Indonesia materi bahasa baku dengan bantuan media pembelajaran game edukasi WhosWants toxbe A Millionaire dibandingkan dengan siswa kelas kontrol. Penelitian ini dirancang menggunakan quasi experimental dengan nonxequivalent control group design. Penelitian ini mengambil populasi dari siswa SMA MuhammadiyahyAl-Kautsar PK Kartasura kelas X. Hasil dari penelitian diperoleh bahwa hasil belajar antara siswa yang menggunakan media pembelajaran game edukasi Who Wantsxto beyA Millionaire mencapaii75,89 dan pada kelas.kontrol hanya 71,69.
Penerapan Pembelajaran Berbasis STEAM (Sience, Technology, Engineering, Art, Mathematic) Pada Mahasiswa Mata Kuliah CAD
Authors: Yuliardi Tri Putra , Basri Irma Yulia , Hernando Fadli , Afrizal Azhar
Published: March 2024

Mata kuliah CAD merupakan mata kuliah yang mengembangkan keterampilan mahasiswa dalam membuat sebuah desain gambar. Pembelajaran CAD teknik otomotif DIII selama ini belum mengarah kepada desain tugas akhir mahasiswa sehingga alat peraga tugas akhir mahasiswa selama ini masih terlihat kurang menarik sehingga perlu adanya pembelajaran yang lebih inovatif. Pembelajaran berbasis STEAM merupakan pembelajaran innovatif abad 21. Pembelajaran STEAM mengintegrasikan lima bidang ilmu dalam proses pembelajaran. Tujuan penelitian ini adalah untuk mendeskripsikan hasil dari penerapan pembelajaran berbasis STEAM pada mata kuliah CAD Teknik Otomotif DIII. Jenis penelitian yang digunakan dalam penelitian ini adalah penelitian tindakan kelas. Berdasarkan hasil penelitian yang dilakukanan dapat disimpulkan bahwa terdapat peningkatan kualitas pembelajaran mahasiswa dengan menerapkan pembelajaran berbasis STEAM pada Mata Kuliah CAD.
Pengembangan Sistem Array dalam Menentukan Nilai Rata-Rata Tinggi Badan
Authors: Marlina Leni
Published: March 2024

Technology that is increasingly sophisticated and continues to be developed with a variety of existing knowledge can easily help human work. The use of this technology can be applied in data management on a large scale, if calculated with manual capabilities it will take a long time to complete. This study aims to help process data on a large scale so that with the help of Del C++ program technology using Array language this kind of problem can be easily solved. Array programming language is a programming language that uses different indexes but the data type is the same. This program functions in determining the average value of an array. data or often referred to as Mean. Mean is the sum of all data and divided by a lot of existing data so that the median or average value of a data is obtained. Many agencies require this programming, especially health installations and educational institutions that are very requires an average component in order to calculate the average height of patients and students whose data is on a large scale so that it is difficult to calculate manually. The use of this program is considered more effective in processing data and finding the middle value of existing data so that objective data is obtained. if it is observed by going directly into the field so that there are no presumptions or prejudices that arise from the data obtained and it is efficient in determining what the mean value.
The specifics of the representation of speech strategies and tactics in educational audio podcasts
Authors: Egoshkina Violetta Aleksandrovna
Published: March 2024

The object of the research is educational audio podcasts presented on the platform Smotrim.ru (Radio Mayak audio podcasts): "100 Minutes" (16 issues), "Literary squabbles" (11 issues), "Understand yourself if you can" (15 issues), "Conversations about Literature" (14 issues), "Diary his Wives" (11 issues), as well as episodes of the audio podcast hosted on the Polka.academy platform – "Between the Lines" (7 issues), published from 2021 to 2023. The subject of the study is the pragmastilistic specificity of media texts represented in empirical material. The purpose of the work is to present various interpretations of the term "audio podcast", to consider definitions of the concepts "speech strategy" and "speech tactics", approaches to their classification, as well as to identify and describe dominant communicative strategies and tactics explicated in educational audio podcasts. To achieve the stated goal, methods of content analysis, linguistic stylistic and pragmastilistic analysis of media texts were applied. Despite the fact that audio podcasts are now in demand among the audience and are presented on various platforms, including on the websites of major radio stations and streaming services, this media phenomenon still needs scientific reflection. Thus, the works of Russian researchers have not yet formulated a generally accepted definition of an audio podcast, nor has a unified point of view been developed on how to consider an audio podcast as a new kind of radio or an independent format. In addition, the communicative and pragmatic specifics of media texts explicated in audio podcasts have been studied very little. This determines the relevance and scientific novelty of the research undertaken. As a result of the analysis of empirical material, dominant speech strategies and tactics implementing them were identified. Conclusions are drawn about the stylistic originality of the analyzed media texts.
The narrative structure of Yu. P. Kazakov's short story "Arcturus the Hound Dog"
Authors: Pyhtina Irina Yur'evna , Bubnova Natal'ya Aleksandrovna
Published: March 2024

The article presents a narrative analysis of Yu.P. Kazakov's short story "Arcturus the Hound Dog", which refers to the animalistic prose of the writer. The main purpose of the analysis is to consider the organization of the narrative in prose, since the narrative features of the work allow us to characterize the writer's idiosyncrasy in detail. Research objectives: to identify the features of the narrative; to get acquainted with the works devoted to the research topic; to turn to the historical and cultural context; identify the types of narrators; correlate the perspective and narrative plan with them. The relevance of the work is due to the fact that more and more studies are interested in narrative structures, as they allow us to determine the nature of the work and the poetics of Kazakov's stories. Despite the fact that many works are devoted to the early story "Arcturus the Hound Dog", not all of them differ in precise terminology and logical conclusions. So, we will attempt to challenge one of the points of view on the analyzed work. The study of the issue was based on a structural and semantic analysis. Formal, biographical and psychological methods were also used in the study. In the course of the work, the features of the narrative of the story were discovered. We came to the following conclusions: 1. The narrative is based on the principle of opposition of perspectives: an "internal - external" point of view (Innenperspektive - Außenperspektive) 2. The writer introduces three types of narrator into the canvas of the text: exegetic (omniscient, taking an indirect part in the action of the story), diegetic (active, directly appearing in the plot as a character) and lyrical (reflective, feeling, which manifests itself more on a spiritual level). The discovery of the latter in the canvas of the text determines the novelty of the study of the literary heritage of Yu. P. Kazakov. 3. Thus, the lyrical type of the narrator determines the poetics of Kazakov's lyrical prose, discovers difficult-to-grasp subtexts in the narrative and introduces a special rhythm into the analyzed story.
Dyskurs edukacyjny w perspektywie ideologicznej. Marzanna Pogorzelska (2023): Odkrywamy ukryte. Konstrukcja i dekonstrukcja neoliberalnego przekazu podręczników szkolnych. Opole: Wydawnictwo Uniwersytetu Opolskiego, 262 ss.
Authors: Nocoń Jolanta
Published: March 2024

W artykule przedstawiono recenzje książki Marzanny Pogorzelskiej pt. Odkrywamy ukryte. Konstrukcja i dekonstrukcja neoliberalnego przekazu podręczników szkolnych. Opole 2013.
Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response
Authors: Hayes Brandon H. , Wang Mai , Zhu Hui , Phan Steven H. , Dooling Lawrence J. , Andrechak Jason C. , Chang Alexander H. , Tobin Michael P. , Ontko Nicholas M. , Marchena Tristan , Discher Dennis E.
Published: March 2024

Chromosomal instability (CIN), a state in which cells undergo mitotic aberrations that generate chromosome copy number variations, generates aneuploidy and is thought to drive cancer evolution. Although associated with poor prognosis and reduced immune response, CIN generates aneuploidy-induced stresses that could be exploited for immunotherapies. In such contexts, macrophages and the CD47-SIRPα checkpoint are understudied. Here, CIN is induced pharmacologically induced in poorly immunogenic B16F10 mouse melanoma cells, generating persistent micronuclei and diverse aneuploidy while skewing macrophages towards an anti-cancer M1-like phenotype, based on RNA-sequencing profiling, surface marker expression and short-term antitumor studies. These results further translate to
in vivo
efficacy: Mice bearing CIN-afflicted tumors with wild-type CD47 levels survive only slightly longer relative to chromosomally stable controls, but long-term survival is maximized when combining macrophage-stimulating anti-tumor IgG opsonization and some form of disruption of the CD47-SIRPα checkpoint. Survivors make multi-epitope,
de novo
anti-cancer IgG that promote macrophage-mediated phagocytosis of CD47 knockout B16F10 cells and suppress tumoroids
in vitro
and growth of tumors
in vivo
. CIN does not greatly affect the level of the IgG response compared to previous studies but does significantly increase survival. These results highlight an unexpected therapeutic benefit from CIN when paired with maximal macrophage anti-cancer activity: an anti-cancer vaccination-like antibody response that can lead to more durable cures and further potentiate cell-mediated acquired immunity.
voyAGEr: free web interface for the analysis of age-related gene expression alterations in human tissues
Authors: Schneider Arthur L. , Martins-Silva Rita , Kaizeler Alexandre , Saraiva-Agostinho Nuno , Barbosa-Morais Nuno L.
Published: March 2024

We herein introduce voyAGEr, an online graphical interface to explore age-related gene expression alterations in 49 human tissues. voyAGEr offers a visualisation and statistical toolkit for the finding and functional exploration of sex– and tissue-specific transcriptomic changes with age. In its conception, we developed a novel bioinformatics pipeline leveraging RNA sequencing data, from the GTEx project, encompassing more than 900 individuals. voyAGEr reveals transcriptomic signatures of the known asynchronous ageing between tissues, allowing the observation of tissue-specific age-periods of major transcriptional changes, associated with alterations in different biological pathways, cellular composition, and disease conditions.Notably, voyAGEr was created to assist researchers with no expertise in bioinformatics, providing a supportive framework for elaborating, testing and refining their hypotheses on the molecular nature of human ageing and its association with pathologies, thereby also aiding in the discovery of novel therapeutic targets. voyAGEr is freely available at https://compbio.imm.medicina.ulisboa.pt/app/voyAGEr.
Type 2 diabetes and fracture risk: deciphering the complex relationship with both genetic and observational evidence
Authors: Zhao Pianpian , Sheng Zhifeng , Xu Lin , Li Peng , Xiao Wenjin , Yuan Chengda , Xu Zhanwei , Yang Mengyuan , Qian Yu , Zhong Jiadong , Gu Jiaxuan , Karasik David , Zheng Hou-Feng
Published: March 2024

The “diabetic bone paradox” suggested that type 2 diabetes (T2D) patients would have higher areal bone mineral density (BMD) but higher fracture risk than individuals without T2D. In this study, we found that the genetically predicted T2D was associated with higher BMD and lower risk of fracture in both wGRS and two-sample MR analyses. We also identified ten genomic loci shared between T2D and fracture, with the top signal at SNP rs4580892 in the intron of gene
RSPO3
. And the higher expression in adipose subcutaneous and higher protein level in plasma of
RSPO3
were associated with increased risk of T2D, but decreased risk of fracture. In the prospective study, T2D was observed to be associated with higher risk of fracture, but BMI mediated 30.2% of the protective effect. However, when stratified by the risk factors secondary to the disease, we observed that the effect of T2D on the risk of fracture decreased when the number of risk factors secondary to T2D decreased, and the association became non-significant if the T2D patients carried none of the risk factors. In conclusion, the genetically determined T2D might not be associated with higher risk of fracture. And the shared genetic architecture between T2D and fracture suggested a top signal around
RSPO3
gene. The observed effect size of T2D on fracture risk decreased if the risk factors secondary to T2D could be eliminated. Therefore, it is important to manage the complications of T2D to prevent the risk of fracture.
Coupling and uncoupling of midline morphogenesis and cell flow in amniote gastrulation
Authors: Asai Rieko , Prakash Vivek N. , Sinha Shubham , Prakash Manu , Mikawa Takashi
Published: March 2024

Large-scale cell flow characterizes gastrulation in animal development. In amniote gastrulation, particularly in avian gastrula, a bilateral vortex-like counter-rotating cell flow, called ‘polonaise movements’, appears along the midline. Here, through experimental manipulations, we addressed relationships between the polonaise movements and morphogenesis of the primitive streak, the earliest midline structure in amniotes. Suppression of the Wnt/planar cell polarity (PCP) signaling pathway maintains the polonaise movements along a deformed primitive streak. Mitotic arrest leads to diminished extension and development of the primitive streak and maintains the early phase of the polonaise movements. Ectopically induced Vg1, an axis-inducing morphogen, generates the polonaise movements, aligned to the induced midline, but disturbs the stereotypical cell flow pattern at the authentic midline. Despite the altered cell flow, induction and extension of the primitive streak are preserved along both authentic and induced midlines. Finally, we show that ectopic axis-inducing morphogen, Vg1, is capable of initiating the polonaise movements without concomitant PS extension under mitotic arrest conditions. These results are consistent with a model wherein primitive streak morphogenesis is required for the maintenance of the polonaise movements, but the polonaise movements are not necessarily responsible for primitive streak morphogenesis. Our data describe a previously undefined relationship between the large-scale cell flow and midline morphogenesis in gastrulation.
Nifuroxazide suppresses PD-L1 expression and enhances the efficacy of radiotherapy in hepatocellular carcinoma
Authors: Zhao Tiesuo , Wei Pengkun , Zhang Congli , Zhou Shijie , Liang Lirui , Guo Shuoshuo , Yin Zhinan , Cheng Sichang , Gan Zerui , Xia Yuanling , Zhang Yongxi , Guo Sheng , Zhong Jiateng , Yang Zishan , Tu Fei , Wang Qianqing , Bai Jin , Ren Feng , Feng Zhiwei , Jia Huijie
Published: March 2024

Radiation therapy is a primary treatment for hepatocellular carcinoma (HCC), but its effectiveness can be diminished by various factors. The over-expression of PD-L1 has been identified as a critical reason for radiotherapy resistance. Previous studies have demonstrated that nifuroxazide exerts antitumor activity by damaging the Stat3 pathway, but its efficacy against PD-L1 has remained unclear. In this study, we investigated whether nifuroxazide could enhance the efficacy of radiotherapy in HCC by reducing PD-L1 expression. Our results showed that nifuroxazide significantly increased the sensitivity of tumor cells to radiation therapy by inhibiting cell proliferation and migration while increasing apoptosis in vitro. Additionally, nifuroxazide attenuated the up-regulation of PD-L1 expression induced by irradiation, which may be associated with increased degradation of PD-L1 through the ubiquitination-proteasome pathway. Furthermore, nifuroxazide greatly enhanced the efficacy of radiation therapy in H22-bearing mice by inhibiting tumor growth, improving survival, boosting the activation of T lymphocytes, and decelerating the ratios of Treg cells in spleens. Importantly, nifuroxazide limited the increased expression of PD-L1 in tumor tissues induced by radiation therapy. This study confirms, for the first time, that nifuroxazide can augment PD-L1 degradation to improve the efficacy of radiation therapy in HCC-bearing mice.
Heron: A Knowledge Graph editor for intuitive implementation of python based experimental pipelines
Authors: Dimitriadis George , Svahn Ella , MacAskill Andrew , Akrami Athena
Published: March 2024

To realise a research project idea, an experimenter faces a series of conflicting design and implementation considerations, regarding both its hardware and software components. For instance, the ease of implementation, in time and expertise, should be balanced against the ease of future reconfigurability and number of ‘black box’ components. Other, often conflicting, considerations include the level of documentation and ease of reproducibility, resource availability as well as access to online communities. To alleviate this balancing act between opposing requirements we present Heron, a new Python-based platform to construct and run experimental and data analysis pipelines. Heron’s main principle is to allow researchers to design and implement the experimental flow as close as possible to their mental schemata of the experiment, in the form of a Knowledge Graph. Heron is designed to increase the implementation speed of experiments (and their subsequent updates), while minimising the number of incorporated black box components. It enhances the readability and reproducibility of the final implementation and allows the use of combinations of hardware and software otherwise impossible or too costly to achieve. Given this, Heron offers itself to sciences whose needs involve experiments with a large number of interconnected hardware and software components like robotics, neuroscience, behavioural sciences, physics, chemistry, environmental science, etc.. It is designed with those experimentalists in mind which: i) Demand full control of their setup. ii) Prefer not to have to choose between hardware and software that run only on a specific chip/operating system combination. iii) Appreciate the ease and speed that high-level languages (e.g. Python) and Graphical User Interfaces (GUIs) offer them. It assumes an intermediate knowledge of the Python language and ecosystem, offering a flexible and powerful way to construct experimental setups. It removes any inaccessible corners, yet keeps implementation costs significantly reduced compared to using lower level languages. Finally, its use results in a much cleaner and easier to understand code base, amicable to documentation and reproducibility efforts.
Distinct T-cell receptor (TCR) gene segment usage and MHC-restriction between foetal and adult thymus
Authors: Rowell Jasmine , Lau Ching-In , Ross Susan , Yanez Diana C , Peña Oscar A , Chain Benny , Crompton Tessa
Published: March 2024

Here, we sequenced rearranged TCRβ and TCRα chain sequences in CD4+CD8+ double positive (DP), CD4+CD8- single positive (SP4) and CD4-CD8+ (SP8) thymocyte populations from the foetus and young adult mouse. We found that life-stage had a greater impact on TCRβ and TCRα gene segment usage than cell-type. Foetal repertoires showed bias towards 3’TRAV and 5’TRAJ rearrangements in all populations, whereas adult repertoires used more 5’TRAV gene segments, suggesting that progressive TCRα rearrangements occur less frequently in foetal DP cells. When we synchronised young adult DP thymocyte differentiation by hydrocortisone treatment the new recovering DP thymocyte population showed more foetal-like 3’TRAV and 5’TRAJ gene segment usage. In foetus we identified less influence of MHC-restriction on α-chain and β-chain combinatorial VxJ usage and CDR1xCDR2 (V region) usage in SP compared to adult, indicating weaker impact of MHC-restriction on the foetal TCR repertoire. The foetal TCRβ repertoire was less diverse, less evenly distributed, with fewer non-template insertions, and all foetal populations contained more clonotypic expansions than adult. The differences between the foetal and adult thymus TCR repertoires are consistent with the foetal thymus producing αβT-cells with properties and functions that are distinct from adult T-cells: their repertoire is less governed by MHC-restriction, with preference for particular gene segment usage, less diverse with more clonotypic expansions, and more closely encoded by genomic sequence.
Lateral/caudal ganglionic eminence makes limited contribution to cortical oligodendrocytes
Authors: Li Jialin , Yang Feihong , Tian Yu , Wang Ziwu , Qi Dashi , Yang Zhengang , Song Jiangang , Ding Jing , Wang Xin , Zhang Zhuangzhi
Published: March 2024

The emergence of myelinating oligodendrocytes represents a pivotal developmental milestone in vertebrates, given their capacity to ensheath axons and facilitate the swift conduction of action potentials. It is widely accepted that cortical oligodendrocyte progenitor cells (OPCs) arise from medial ganglionic eminence (MGE), lateral/caudal ganglionic eminence (LGE/CGE), and cortical radial glial cells (RGCs). Here, we used two different fate mapping strategies to challenge the established notion that the LGE generates cortical OPCs. Furthermore, we used a Cre/loxP-dependent exclusion strategy to reveal that the LGE/CGE does not give rise to cortical OPCs. Additionally, we showed that specifically eliminating MGE-derived OPCs leads to a significant reduction of cortical OPCs. Together, our findings indicate that the LGE does not generate cortical OPCs, contrary to previous beliefs. These findings provide a new view of the developmental origins of cortical OPCs and a valuable foundation for future research on both normal development and oligodendrocyte-related disease.
The genetic architecture of the load linked to dominant and recessive self-incompatibility alleles in Arabidopsis halleri and A. lyrata
Authors: Le Veve Audrey , Genete Mathieu , Lepers-Blassiau Christelle , Ponitzki Chloé , Céline Poux , Vekemans Xavier , Durand Eleonore , Castric Vincent
Published: March 2024

The long-term balancing selection acting on mating-types or sex determining genes is expected to lead to the accumulation of deleterious mutations in the tightly linked chromosomal segments that are locally “sheltered” from purifying selection. However, the factors determining the extent of this accumulation are poorly understood. Here, we take advantage of the large number of alleles at the sporophytic self-incompatibility system of the Brassicaceae along a complex dominance hierarchy to evaluate how the pace at which linked deleterious mutations accumulate varies with the intensity of balancing selection. We first experimentally measured the phenotypic manifestation of the linked load at three different levels of the dominance hierarchy. We then sequenced and phased polymorphisms in the chromosomal regions linked to 126 distinct copies of
S
-alleles in two populations of
Arabidopsis halleri
and three populations of
A. lyrata
. We find that linkage to the
S
-locus locally distorts phylogenies over about 10-30kb along the chromosome. The more intense balancing selection on dominant
S
-alleles results in greater fixation of deleterious mutations, while recessive
S
-alleles accumulate more linked deleterious mutations that are segregating. Hence, the structure rather than the overall magnitude of the linked genetic load differs between dominant and recessive
S
-alleles. Our results have consequences for the long-term evolution of new
S
-alleles, the evolution of dominance modifiers between them, and raise the question of why the non-recombining regions of some sex and mating type chromosomes expand over evolutionary times while others, such as that the
S
-locus of the Brassicaceae, remain restricted to small chromosomal regions.
Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosis
Authors: Xie Xufeng , Chen Xi , Zhang Shilei , Liu Jiuxi , Zhang Wenlong , Cao Yongguo
Published: March 2024

Leptospirosis is an emerging infectious disease caused by pathogenic Leptospira spp. Humans and some mammals can develop severe forms of leptospirosis accompanied by a dysregulated inflammatory response, which often results in death. The gut microbiota has been increasingly recognized as a vital element in systemic health. However, the precise role of the gut microbiota in severe leptospirosis is still unknown. Here, we aimed to explore the function and potential mechanisms of the gut microbiota in a hamster model of severe leptospirosis. Our study showed that leptospires were able to multiply in the intestine, cause pathological injury, and induce intestinal and systemic inflammatory responses. 16S rRNA gene sequencing analysis revealed that Leptospira infection changed the composition of the gut microbiota of hamsters with an expansion of Proteobacteria. In addition, gut barrier permeability was increased after infection, as reflected by a decrease in the expression of tight junctions. Translocated Proteobacteria were found in the intestinal epithelium of moribund hamsters, as determined by fluorescence in situ hybridization, with elevated lipopolysaccharide (LPS) levels in the serum. Moreover, gut microbiota depletion reduced the survival time, increased the leptospiral load, and promoted the expression of proinflammatory cytokines after Leptospira infection. Intriguingly, fecal filtration and serum from moribund hamsters both increased the transcription of TNF-α, IL-1β, IL-10, and TLR4 in macrophages compared with those from uninfected hamsters. These stimulating activities were inhibited by LPS neutralization using polymyxin B. Based on our findings, we identified an LPS neutralization therapy that significantly improved the survival rates in severe leptospirosis when used in combination with antibiotic therapy or polyclonal antibody therapy. In conclusion, our study not only uncovers the role of the gut microbiota in severe leptospirosis but also provides a therapeutic strategy for severe leptospirosis.
Investigating the epidemiological relevance of secretory otitis media and neighboring organ diseases through an Internet search
Authors: Guo Cheng , Pan Linlin , Chen Ling , Xie Jinghua , Liang Zhuozheng , Huang Yongjin , He Long
Published: March 2024

Background

This study examined the epidemiological correlations between secretory otitis media (SOM) and diseases of neighboring organs. We measured changes in disease incidences during the 2020 COVID-19 pandemic using Internet big data spanning from 2011 to 2021.

Methods

This study used the Baidu Index (BI) to determine the search volume for the terms “secretory otitis media (SOM)”, “tonsillitis”, “pharyngolaryngitis”, “adenoid hypertrophy (AH)”, “nasopharyngeal carcinoma (NPC)”, “nasal septum deviation (NSD)”, “rhinosinusitis”, “allergic rhinitis (AR)”, and “gastroesophageal reflux disease (GERD)” in Mandarin from January 2011 to December 2021. The correlations between these terms were analyzed using Spearman’s correlation coefficients. The results were compared search data from 2019 and 2021 to assess the effects of isolation on SOM in 2020.

Results

The seasonal variations trends of SOM and other diseases coincided well (P < 0.05), except for AR. During the 11-year timeframe, the monthly searches for rhinosinusitis, NSD, tonsillitis, pharyngolaryngitis, and NPC were statistically correlated with SOM (R = 0.825, 0.594, 0.650, 0.636, 0.664, respectively; P < 0.05). No correlation was found between SOM and AR, SOM and AH, or SOM and GERD (R =  − 0.028, R = 0.259, R = 0.014, respectively, P > 0.05). The total search volumes for SOM, rhinosinusitis, NPC, and AH decreased in 2020 compared to 2019.

Discussion

SOM exhibited a discernible epidemiological connection with rhinosinusitis, nasal septal deviation (NSD), tonsillitis, pharyngolaryngitis, and nasopharyngeal carcinoma (NPC). A decrease in public gatherings was observed to effectively reduce the incidences of SOM. This underscores the pivotal role of social measures in influencing the prevalence of SOM and emphasizes the intricate interplay between SOM and various associated health factors, with implications for public health strategies.
Whistleblowing as a security issue in Hungarian organizations from 2023
Authors: Ilona Mizser Csilla
Published: March 2024

This study aims to provide an overview of the result of the legislative intention, which also set a mandatory deadline for the application of large companies employing more than 250 people, since they must apply Act XXV of 2023 starting from July 24, 2023, on complaints, public interest reports, and the rules on whistleblowing. The purpose of this article is to point out, based on the review of international scientific literature and secondary research, that whistleblowing and reporting are especially supporting tools for organizations, workplaces, employees, and employers. The aim is to emphasize the advantages of organizational security. Presentation of the law created with regard to Hungary's international legal and European Union legal obligations undertaken in connection with actions against corruption. An exhaustive review of the literature on whistleblowing research papers has been conducted. A second study was conducted on regulatory documents, commentaries, and studies. The added value of the paper reflects the relevant legislation and scientific findings in one paper. This paper addresses leaders, employers, employees, university lecturers, and researchers.
O profissional de secretariado executivo e suas contribuições na comunicação interna de uma empresa do ramo farmacêutico
Authors: Moreira Allan James , Rocha Carla Marlana , Alves Carlos Roberto , Alencar Venan
Published: March 2024

Este artigo busca compreender a atuação do profissional de Secretariado Executivo e suas atribuições no processo de comunicação de uma empresa do ramo farmacêutico em Guarapuava-PR. O objetivo geral desta pesquisa é identificar de que maneira este profissional pode usar das atribuições presentes no eixo da assessoria executiva para trazer melhorias no processo de comunicação adotado pela empresa. Buscamos compreender em quais aspectos a assessoria executiva pode auxiliar no contexto organizacional. O trabalho se classificou como um estudo de caso e, para análise dos dados, utilizamos a teoria proposta por Charaudeau (2008 [1984]). A partir dela, consideramos diferentes situações de comunicação obtidas por meio de respostas colhidas em entrevista com o gestor da rede farmacêutica. Identificamos que as características descritas para o profissional de Secretariado Executivo vão ao encontro das soluções necessárias na resolução dos problemas levantados nas situações identificadas.