bluuebunny/crossref_metadata_2025 huggingface dataset:
Axial Compressive Behavior of CFRP and MWCNT Incorporated GFRP Confined Concrete Cylinders after Exposure to Various Aggressive Environments
Authors: Kavitha Sruthi Sreekumar , Madhavan Mini K. , Jayanarayanan Karingamanna , Sarker Prabir Kumar
Published: August 2024

Fiber-reinforced polymer confinement is considered to be effective in the retrofitting of concrete structures. The current study explores the effectiveness of one- and two-layer carbon fiber reinforced polymer (CFRP) and multiwalled carbon nanotube (MWCNT) incorporated three-layer glass fiber reinforced polymer (GFRP) confinement on concrete cylinders under aggressive exposures, such as acid, alkaline, marine, water, and elevated temperatures. At 1 wt.% MWCNT by weight of the epoxy matrix, mechanical characteristics of the laminate show a significant improvement. In the case of acid exposure, the axial load-carrying capacity of concrete specimens with single-layer CFRP confinement was equal to that of MWCNT incorporated three-layer GFRP confinement (GF3C1-AC). The axial strain of GF3C1-AC was 23% and 12% higher than one and two-layer CFRP confinement. After exposure at 400 °C, in comparison with one- and two-layer CFRP confinement, the axial strain of MWCNT incorporated three-layer GFRP confined specimens increased by 50% and 20%, respectively, which proved the efficacy of MWCNT as a heat-resistant nanofiller. The ultrasonic pulse velocity (UPV) test indicates that the confinement system protects the concrete core from sudden failure by impeding crack propagation. The test results proved that the MWCNT incorporated FRP system can be considered as a prospective substitute for CFRP systems for retrofitting applications in severe environmental conditions.
At What Point in the Menstrual Cycle Are the Pelvic Floor Muscles at Their Weakest?
Authors: Ojedo-Martín Cristina , Rodríguez-López Elena Sonsoles , Acevedo-Gómez María Barbaño , Úbeda-D’Ocasar Edurne , de-Diego María Victoria , Lara Beatriz
Published: August 2024

Pelvic floor muscle (PFM) strength is a critical factor for optimal pelvic floor function. Fluctuations in strength values based on different phases of the menstrual cycle (MC) could signify a need for a paradigm shift in evaluating, approaching, and planning training. This research aims to examine and contrast the pelvic floor muscle strength during different phases of the menstrual cycle. A prospective observational study employing digital assessment with the modified Oxford scale and vaginal dynamometry measurements was performed, in order to assess the baseline strength and the contraction strength of the PFMs in eumenorrheic females at three different phases of the MC: the early follicular phase (EFP), the late follicular phase (LFP), and the mid-luteal phase (MLP). During two complete cycles, tympanic temperature and body weight were measured and the urinary luteinizing hormone concentration was tested to determine the time of ovulation. In total, 216 dynamometric measurements of PFM strength were obtained from eighteen nulliparous women (25.72 ± 5.03 years). There were no differences between the baseline strength (p = 0.886) and the contraction strength (p = 0.756) with the dynamometric speculum. In the post hoc analysis, the baseline strength, contraction strength, and strength showed no significant differences between MC phases. As no differences in PFM strength in women were found, the PFMs do not seem to be weaker at any time during the menstrual cycle. It appears that the assessment, establishment, and monitoring of a PFM training program could be initiated at any point in the cycle.
Efficiency and Microstructural Forecasts in Friction Stir Extrusion Compared to Traditional Hot Extrusion of AA6061
Authors: Bocchi Sara , Zambelli Marco , D’Urso Gianluca , Giardini Claudio
Published: August 2024

Conventional aluminum recycling consumes a substantial amount of energy and has a negative impact on secondary alloys. To address this challenging topic, Friction Stir Extrusion has been patented, which represents an innovative solid-state recycling technique that enables the direct extrusion of components from recyclable materials. In recent years, developing simulation models for Friction Stir Extrusion has become essential for gaining a deeper understanding of its underlying physics. Simultaneously, control of the microstructure evolution of extruded profiles is required, as it has a considerable influence on mechanical properties. This research involves a single Lagrangian model, adapted for both the FSE and the traditional hot extrusion processes. The simulations explored various rotational speeds and feed rates, revealing significant effects on grain size and bonding quality. To this model were applied different sub-routines, to investigate the impact of the FSE process with respect to the traditional hot extrusion process in terms of energy demands, quality and microstructure of the extruded pieces. The findings demonstrated that optimal grain refinement occurs at intermediate rotational speeds (600–800 rpm) combined with lower feed rates (1 mm/s). The energy analyses indicated that FSE requires lower total energy compared to traditional hot extrusion, primarily due to the reduced axial thrust and more efficient thermal management. As a result, it was possible to ensure the ability of the developed simulative model to be fully adapted for both processes and to forecast the microstructural changes directly during the process and not only at the end of the extrusion. The study concludes that FSE is a highly efficient method for producing high-quality extruded rods, with the developed simulation model providing valuable insights for process optimization. The model’s adaptability to various starting materials and conditions highlights its potential for broader applications in extrusion technology.
Study of Physical and Mechanical Relationships during the Natural Dewatering of River Sediments and a Kaolin
Authors: Azaiez Dalel , Boullosa Allariz Beatriz , Levacher Daniel
Published: August 2024

This paper investigated the relationships of some physical and mechanical parameters of sediments and a typical clay during a natural dewatering process. Four sediments from different French river dams sampled by the Électricité De France group (EDF group) and a commercial kaolin clay used for comparative purposes were the focus of this study. Continuous dewatering was monitored in a laboratory by quantifying the percentage of water remaining in sediments or clay, drained and evaporated. Undrained shear strength was also assessed during the sediment or clay dewatering process, using the laboratory vane shear test. The samples were controlled along different dimensions during the dewatering process throughout the whole experiment. The results showed a certain interdependence between the physical parameters and the water content (ω), which was normalized by the liquidity limit (ω/LL) over time. This led to sigmoidal and exponential correlations when considering the percentage of water drained. The percentage of water remaining in the sediments or clay was characterized using the normalized water content, leading to exponential and power correlations. Both exponential and linear correlations were perfect for describing the evolution of the percentage of water evaporated. Other correlations were established for variations in void index, dry unit weight/solid unit weight ratio and undrained shear strength during the dewatering process.
Dynamics of Physiological Properties and Endophytic Fungal Communities in the Xylem of Aquilaria sinensis (Lour.) with Different Induction Times
Authors: Zhang Qingqing , Li Rongrong , Lin Yang , Zhao Weiwei , Lin Qiang , Ouyang Lei , Pang Shengjiang , Zeng Huahao
Published: August 2024

Xylem-associated fungus can secrete many secondary metabolites to help Aquilaria trees resist various stresses and play a crucial role in facilitating agarwood formation. However, the dynamics of endophytic fungi in Aquilaria sinensis xylem after artificial induction have not been fully elaborated. Endophytic fungi communities and xylem physio-biochemical properties were examined before and after induction with an inorganic salt solution, including four different times (pre-induction (0M), the third (3M), sixth (6M) and ninth (9M) month after induction treatment). The relationships between fungal diversity and physio-biochemical indices were evaluated. The results showed that superoxide dismutase (SOD) and peroxidase (POD) activities, malondialdehyde (MDA) and soluble sugar content first increased and then decreased with induction time, while starch was heavily consumed after induction treatment. Endophytic fungal diversity was significantly lower after induction treatment than before, but the species richness was promoted. Fungal β-diversity was also clustered into four groups according to different times. Core species shifted from rare to dominant taxa with induction time, and growing species interactions in the network indicate a gradual complication of fungal community structure. Endophytic fungi diversity and potential functions were closely related to physicochemical indices that had less effect on the relative abundance of the dominant species. These findings help assess the regulatory mechanisms of microorganisms that expedite agarwood formation after artificial induction.
Does Treatment of Adolescent Fractures Differ between Specialties? A Survey among Pediatric and Trauma Surgeons
Authors: Hanke Alexander , Scheerer-Harbauer Eva , Wulbrand Christian , Memmel Clemens
Published: August 2024

From a traumatological point of view, adolescents (12–18 years) represent a special group of patients. This is due to their biomechanical characteristics being between pediatric and adult fracture types. In Germany, they are treated by both pediatric and trauma surgeons. For this survey, seven cases of adolescent fractures were evaluated by both pediatric and trauma surgeons and their preferred treatment options were raised. The questionnaires were completed anonymously. Additionally, information on the specialty and years of experience were asked. In total, 126 valid questionnaires were obtained (from 78 pediatric and 48 trauma surgeons). The respondents’ mean clinical experience was high (71.5% stated more than 10 years of surgical experience). For every single exemplary case, a significant difference in therapy decisions between the groups could be found. For the demonstrated seven cases, a tendency toward more operative and more invasive treatments was found with trauma surgeons compared to pediatric surgeons. On the other hand, there was a risk of underestimating the severity of fracture entities similar to adult fractures in pediatric surgeons. Overall, a continuous interdisciplinary exchange between both surgical specialties is necessary to ensure optimal treatment for adolescent fractures and to develop guidelines in the future.
Does the Upgrading of Development Zones Improve Land Use Efficiency under the Net-Zero Carbon City Goal? Prefectural-Level Evidence from Quasi-Natural Experiments in China
Authors: Rao Jinguo , Zhang Xiaosong , Zhai Duanqiang
Published: August 2024

Given the challenges of restricted land resources and net-zero carbon city initiatives, it is crucial to seek a balance between intensive land use and carbon neutrality during the construction of development zones. By incorporating net-zero carbon balance into the land use efficiency evaluation system and utilizing a quasi-natural experiment based on the 2009 provincial development zone upgrading policy, this study investigates the policy’s impact on urban land use efficiency under the net-zero carbon city goal. The study finds that the upgrading of provincial development zones significantly enhances urban land use efficiency, while exhibiting the dual effects of reducing carbon emissions and increasing carbon sinks. Mechanism analysis reveals that the upgrade policy improves land use efficiency by enhancing land-use and environmental regulations, optimizing resource allocation, and fostering green technological innovation. Heterogeneity analyses show that the policy effect is more significant in eastern and central cities, with the impact being strongest in central cities. Additionally, the impact of upgrading to a national high-tech development zone is greater than that of upgrading to a national economic development zone. This article provides insights into how to use industrial policies effectively to achieve intensive land use and high-quality development while aiming for carbon neutrality.
Data Quality of National Monitoring Schemes: Filling the Gap between Specialists and the General Public
Authors: Bergerot Benjamin , Fontaine Benoît
Published: August 2024

Worldwide, large-scale biodiversity monitoring schemes are developing and involve many non-specialist volunteers. If the opening of schemes to non-specialists allows for the gathering of huge amounts of data, their quality represents a controversial issue. In the framework of the French Garden Butterfly Observatory (FGBO), we studied non-specialist volunteer identification errors based on identifications provided during a one-shot experiment. With 3492 butterfly pictures sent by 554 non-specialist volunteers, we directly measured identification errors and misidentification rates for each butterfly species or species group targeted by the FGBO. The results showed that when non-specialist volunteers identified butterflies at the species level, identification errors (i.e., the misidentification rate) reached 20.9%. It was only 5.0% when FGBO species groups were used. This study provides novel insights into the trade-off between data quantity and quality provided by non-specialist volunteers and shows that if protocols, research questions and identification levels are adapted, participatory monitoring schemes relying on non-specialists represent a powerful and reliable tool to study common species at a large scale and on a long-term basis.
Zooplankton Assemblages of an Argentinean Saline Lake during Three Contrasting Hydroperiods and a Comparison with Hatching Experiments
Authors: Echaniz Santiago Andrés , Vignatti Alicia María , Cabrera Gabriela Cecilia
Published: August 2024

Many saline lakes are temporary, with large variations in salinity, and their biota is adapted to withstand unfavorable periods. Utracan Lake, in a protected area in central Argentina, was studied on three occasions under different environmental conditions. In 2007, depth and salinity were 2 m and 33 g/L, and six species were recorded in the zooplankton. In 2009–2010, its maximum depth was 0.3 m, its salinity exceeded 230 g/L, and only Artemia persimilis was recorded. Field studies to compare the active zooplankton of a third period were combined with laboratory tests to ascertain the composition of the egg bank (flotation with sucrose) and zooplankton succession (hatching from sediments). In 2017–2018 (third period), the depth and salinity were 1.75 ± 0.17 m and 47.19 ± 11.40 g/L, respectively. Five species were recorded, and A. persimilis was found coexisting with cladocerans, copepods, and rotifers. Brachionus plicatilis, Hexarthra fennica, Boeckella poopoensis, A. persimilis, and a single specimen of Moina eugeniae were recorded in hatching experiments; however, the latter species was not recorded again. No cladoceran ephippia were recorded in the flotation tests. Salt accumulation on the sediments during the Utracan drought (2010–2016) would have deteriorated the ephippia. The register of M. eugeniae in 2017–2018 could be largely because of recolonization by waterfowl. The conservation of Utracan Lake is therefore advisable, and the same goes for other nearby saline lakes, which can act as sources of propagules that cross terrestrial areas through transport by wind or zoochory.
Simulation-Based Optimization of Truck Appointment Systems in Container Terminals: A Dual Transactions Approach with Improved Congestion Factor Representation
Authors: Bett Davies K. , Ali Islam , Gheith Mohamed , Eltawil Amr
Published: August 2024

Background: Container terminals (CTs) have constantly administered truck appointment systems (TASs) to effectively accomplish the planning and scheduling of drayage operations. However, since the operations in the gate and yard area of a CT are stochastic, there is a need to incorporate uncertainty during the development and execution of appointment schedules. Further, the situation is complicated by disruptions in the arrival of external trucks (ETs) during transport, which results in congestion at the port due to unbalanced arrivals. In the wake of Industry 4.0, simulation can be used to test and investigate the present CT configurations for possible improvements. Methods: This paper presents a simulation optimization (SO) and simulation-based optimization (SBO) iteration framework which adopts a dual transactions approach to minimize the gate operation costs and establish the relationship between productivity and service time while considering congestion in the yard area. It integrates the use of both the developed discrete event simulation (DES) and a mixed integer programming (MIP) model from the literature to iteratively generate an improved schedule. The key performance indicators considered include the truck turnaround time (TTT) and the average time the trucks spend at each yard block (YB). The proposed approach was verified using input parameters from the literature. Results: The findings from the SO experiments indicate that, at most, two gates were required to be opened at each time window (TW), yielding an average minimum operating cost of USD 335.31. Meanwhile, results from the SBO iteration experiment indicate an inverse relationship between productivity factor (PF) values and yard crane (YC) service time. Conclusions: Overall, the findings provided an informed understanding of the need for dynamic scheduling of available resources in the yard to cut down on the gate operating costs. Further, the presented two methodologies can be incorporated with Industry 4.0 technologies to design digital twins for use in conventional CT by planners at an operational level as a decision-support tool.
Analysis of Chip Morphology in Heavy Milling of 508III Steel Considering Different Tool Wear Conditions
Authors: Guan Rui , Cheng Yaonan , Xue Jing , Zhou Shilong , Zhou Xingwei , Zhai Wenjie
Published: August 2024

During the process of chip formation, the chip is subjected to extrusion pressure, friction, heat, and a strong chemical reaction. The chip’s macro and micro morphology, to a certain extent, reflect the condition of the tool during the cutting procedure. Therefore, researching the macroscopic and microscopic morphology of the chip’s surface in response to different tool wear conditions is of great significance to reproducing the cutting condition and analyzing the tool wear mechanism. This paper focuses on the chips formed by milling the difficult-to-machine material 508III high-strength steel. Firstly, the 508III steel milling experiment is carried out at the actual machining site to collect chip data under different tool wear conditions. Next, the free surface morphology of chips and the bottom surface morphology of chips are analyzed. Further, the chip edges are investigated, and their causes are analyzed. Finally, heavy milling 508III steel chip curl morphology analysis is performed. The research results play important roles in revealing the mechanism of tool wear and the relationship between chip morphology and tool wear. This information can be used to provide theoretical and technical support for monitoring the tool wear status based on chip morphology.
Multi-Objective Optimization of Injection Molding Parameters for Manufacturing Thin-Walled Composite Connector Terminals
Authors: Tan Mingbo , Peng Size , Huo Yingfei , Li Maojun
Published: August 2024

The rapid development of new energy vehicles demands significant improvements in connector structures and performance standards. Wire harness connectors, crucial for linking various electrical components, face challenges due to their small size and thin-walled structure, which can lead to dimensional shrinkage and warping during injection molding. To address these issues, this study optimizes the injection molding process by fine-tuning parameters such as melt temperature, mold temperature, injection time, holding pressure/time, and cooling time. By integrating the Taguchi method with grey relational analysis, the study enhances the molding process for thin-walled composite connectors. This combined approach provides a comprehensive framework for optimizing multiple quality objectives and improving the overall performance of injection-molded composite components.
Modeling Strain Hardening of Metallic Materials with Sigmoidal Function Considering Temperature and Strain Rate Effects
Authors: Pan Boyu , Shen Fuhui , Sampathkumar Sanjay Raghav , Münstermann Sebastian
Published: August 2024

This study uses a sigmoidal function to describe the plastic strain hardening of metallic materials, considering temperature and strain rate effects. The effectiveness of this approach is evaluated and systematically compared with other hardening laws. Incorporating temperature and strain rate effects into the parameters of this sigmoidal-type hardening law enables a more precise description and prediction of the plastic deformation of materials under different combinations of temperature and strain rate. The temperature effect is coupled using a simplified Arrhenius model, and the strain rate effect is coupled with a modified Johnson–Cook model. The sigmoidal-type hardening law is integrated with an asymmetric yield criterion to address complex behavior, such as anisotropy and strength differential effects. The calibration and validation of the constitutive model involve examining uniaxial tensile/compressive flow curves in various directions and biaxial tensile/compressive flow curves for diverse metallic alloys, proving the proposed model’s broad applicability.
Bending Collapse and Energy Absorption of Dual-Phase Lattice Structures
Authors: Chen Zihao , Zhu Zijie , Li Bangzhen , Leng Kehua , Yu Min , Huang Zhixin , Li Ying
Published: August 2024

A dual-phase lattice structure composed of mixed units with hard and soft phase characteristics is proposed in this work. The proposed lattice structure has high specific energy absorption and high compressive strength. The load response and energy absorption characteristics under bending loads were studied through three-point bending tests and numerical analysis methods. The research results indicate that although the deformation modes of the given lattice structure are the same, the dual-phase design strategy significantly improves the bending performance of the lattice structure: the bending modulus is increased by 744.7%, and the specific energy absorption is increased by 243.5%.
Rheological Performance and Differences between Laboratory-Aged and RAP Bitumen
Authors: Baldino Noemi , Mileti Olga , Marchesano Ylenia Maria , Lupi Francesca R. , Gabriele Domenico , Paolini Massimo
Published: August 2024

Traditional recycled asphalt pavement (RAP) binder extraction is not a cost-effective and sustainable option for a quick field study because it requires the use of a huge amount of solvent. Hence, most of the studies on asphalt pavement are carried out with laboratory-aged bitumen in accordance with well-established procedures, i.e., the pressure aging vessel (PAV). Unfortunately, some studies highlight the differences between bitumen aged in the laboratory and in service because it is difficult to reproduce extreme conditions such as real conditions, both atmospheric and load; and this also affects the choice and use of rejuvenators, sometimes compromising the interpretation of results. This study aims to compare the thermo-rheological behavior of a 70/100 bitumen aged with the PAV and two different binders extracted by RAPs. The rheological performances of bitumens were compared in temperature and by dynamic oscillatory tests and steady-state tests, resulting in strength and viscosity values higher for samples with RAP binders compared to the PAV sample. The same bitumens were tested with the addition of a 3% w/w of soybean oil (SO). The results show a decrease in the moduli and viscosity at all the temperatures investigated when SO is added to the laboratory-aged bitumen, while no appreciable differences are evident on naturally aged samples added with SO. Differences were evaluated in terms of cross-over frequency and rheological parameters. Furthermore, the SO effect showed substantial differences, especially in viscosity values, indicating that the study of regenerated or modified bitumen from aged bitumen still requires study, as current standard techniques and procedures cannot emulate real aging conditions well.
Strength and Fracture Toughness of TIG- and Laser-Welded Joints of Low Carbon Ferritic Steels
Authors: Pała Tadeusz , Wciślik Wiktor
Published: August 2024

This paper presents the results of experimental testing of joints welded using conventional TIG and laser methods. The welded components were sheets of the low-carbon steels 13CrMo4-5 and 16Mo3. Welded joints were made using different levels of linear welding energy. In the case of laser welding, a bifocal beam with longitudinal positioning of the focal lengths in relation to the welding direction was used. Experimental tests on welded joints included a bending test and determination of hardness distribution, mechanical properties, and fracture toughness, as well as microstructural research in the material of the various joint zones. Based on the determined strength characteristics, the true stress–strain relationships were defined, and a numerical model of the laser joints was developed in Abaqus 6.12-3. The modelled joint was subjected to loading to determine the most stressed areas of the joints. The numerical results were compared with those obtained using GOM’s Aramis 3D 5M digital image correlation system. The system used made it possible to record displacements on the surface of the analysed joints in real time. Good agreement was obtained between the strain fields calculated numerically and those recorded using the Aramis 3D 5M video system. The numerical calculations provided information on the strains and stresses occurring inside the analysed joint during loading. It was found that the welded joints were characterised by increased hardness and high strength properties in relation to the base material. The bending test of the laser-welded joints gave a positive result—no cracks were observed on the face or root of the weld. The fracture toughness of the joint zones is slightly lower in relation to that of the base material, but no brittle fracture was observed.
Simulation and Algorithmic Optimization of the Cutting Process for the Green Machining of PM Green Compacts
Authors: Zhang Yuchen , Yang Dayong , Zeng Lingxin , Zhang Zhiyang , Li Shuping
Published: August 2024

Powder metallurgy (PM) technology is extensively employed in the manufacturing sector, yet its processing presents numerous challenges. To alleviate these difficulties, green machining of PM green compacts has emerged as an effective approach. The aim of this research is to explore the deformation features of green compacts and assess the impact of various machining parameters on the force of cutting. The cutting variables for compacts of PM green were modeled, and the cutting process was analyzed using Abaqus (2022) software. Subsequently, the orthogonal test ANOVA method was utilized to evaluate the significance of each parameter for the cutting force. Optimization of the machining parameters was then achieved through a genetic algorithm for neural network optimization. The investigation revealed that PM green compacts, which are brittle, undergo a plastic deformation stage during cutting and deviate from the traditional model for brittle materials. The findings indicate that cutting thickness exerts the most substantial influence on the cutting force, whereas the speed of cutting, the tool rake angle, and the radius of the rounded edge exert minimal influence. The optimal parameter combination for the cutting of PM green compacts was determined via a genetic algorithm for neural network optimization, yielding a cutting force of 174.998 N at a cutting thickness of 0.15 mm, a cutting speed of 20 m/min, a tool rake angle of 10°, and a radius of the rounded edge of 25 μm, with a discrepancy of 4.05% from the actual measurement.
G-Quadruplex DNA as a Macromolecular Target for Semi-Synthetic Isoflavones Bearing B-Ring Tosylation
Authors: Ribaudo Giovanni , Anyanwu Margrate , Giannangeli Matteo , Oselladore Erika , Ongaro Alberto , Memo Maurizio , Gianoncelli Alessandra
Published: August 2024

Guanine-rich sequences of nucleic acids, including DNA and RNA, are known to fold into non-canonical structures named G-quadruplexes (G4s). Such arrangements of these macromolecular polymers are mainly located in telomeres and in promoter regions of oncogenes and, for this reason, they represent a potential target for compounds with therapeutic applications. In fact, the ligand-mediated stabilization of G4s inhibits telomerase and the activity of transcriptional machinery and counteracts cancer cell immortalization. Flavonoids, along with other classes of small molecules, have been previously tested for their ability to stabilize G4s, but the mechanism of their interaction has not been fully elucidated. In the current work, we report a multi-technique investigation on the binding of tosylated isoflavones obtained by the B-ring modification of compounds from Maclura pomifera to a telomeric DNA sequence. Our study demonstrates that such derivatization leads to compounds showing lower binding affinity but with an increased selectivity toward G4 with respect to double-stranded DNA. The binding mode to the macromolecular target G4 was studied by combining results from electrospray mass spectrometry binding studies, nuclear magnetic resonance experiments and computational simulations. Overall, our findings show that tosylation influences the selectivity toward the macromolecular target by affecting the interaction mode with the nucleic acid.
Structural, Thermal and Mechanical Assessment of Green Compounds with Natural Rubber
Authors: Colom Xavier , Sans Jordi , de Bruijn Frederic , Carrillo Fernando , Cañavate Javier
Published: August 2024

The inadequate disposal of tires poses a significant threat to human health and requires effective recycling solutions. The crosslinked structure of rubber, formed through sulfur bridges during vulcanization, presents a major challenge for recycling because it prevents the rubber scraps from being reshaped thermoplastically. Reclaiming or devulcanization aims to reverse this crosslinking, allowing waste rubber to be transformed into products that can be reprocessed and revulcanized, thereby saving costs and preserving resources. Microwave technology shows promise for devulcanization due to its ability to break sulfur crosslinks. In this study, we investigate the devulcanization of ground tire rubber (GTR) through a combined process applied to samples from both car and truck tires subjected to varying periods of microwave irradiation (0, 3, 5 and 10 min). The devulcanized GTR was then blended with natural rubber (NR) and underwent a new vulcanization process, simulating recycling for novel applications. The GTR was mixed with NR in proportions of 0, 10, 30 and 50 parts per hundred rubber (phr). This study also examines the differences between the GTR from car tires and GTR from truck tires. The results showed that the treatment effectively breaks the crosslinks in the GTR, creating double bonds (C=C) and improving the mechanical properties of the revulcanized samples. The crosslinking density and related properties of the samples increased with treatment time, reaching a maximum at 5 min of microwave treatment, followed by a decrease at 10 min. Additionally, the incorporation of GTR enhanced the thermal stability of the resulting materials.
GaitSTAR: Spatial–Temporal Attention-Based Feature-Reweighting Architecture for Human Gait Recognition
Authors: Bilal Muhammad , Jianbiao He , Mushtaq Husnain , Asim Muhammad , Ali Gauhar , ElAffendi Mohammed
Published: August 2024

Human gait recognition (HGR) leverages unique gait patterns to identify individuals, but the effectiveness of this technique can be hindered due to various factors such as carrying conditions, foot shadows, clothing variations, and changes in viewing angles. Traditional silhouette-based systems often neglect the critical role of instantaneous gait motion, which is essential for distinguishing individuals with similar features. We introduce the ”Enhanced Gait Feature Extraction Framework (GaitSTAR)”, a novel method that incorporates dynamic feature weighting through the discriminant analysis of temporal and spatial features within a channel-wise architecture. Key innovations in GaitSTAR include dynamic stride flow representation (DSFR) to address silhouette distortion, a transformer-based feature set transformation (FST) for integrating image-level features into set-level features, and dynamic feature reweighting (DFR) for capturing long-range interactions. DFR enhances contextual understanding and improves detection accuracy by computing attention distributions across channel dimensions. Empirical evaluations show that GaitSTAR achieves impressive accuracies of 98.5%, 98.0%, and 92.7% under NM, BG, and CL conditions, respectively, with the CASIA-B dataset; 67.3% with the CASIA-C dataset; and 54.21% with the Gait3D dataset. Despite its complexity, GaitSTAR demonstrates a favorable balance between accuracy and computational efficiency, making it a powerful tool for biometric identification based on gait patterns.
Comparative Analysis of Tentacle Extract and Nematocyst Venom: Toxicity, Mechanism, and Potential Intervention in the Giant Jellyfish Nemopilema nomurai
Authors: Geng Xiao-Yu , Wang Ming-Ke , Hou Xiao-Chuan , Wang Zeng-Fa , Wang Yi , Zhang Die-Yu , Danso Blessing , Wei Dun-Biao , Shou Zhao-Yong , Xiao Liang , Yang Ji-Shun
Published: August 2024

The giant jellyfish Nemopilema nomurai sting can cause local and systemic reactions; however, comparative analysis of the tentacle extract (TE) and nematocyst venom extract (NV), and its toxicity, mechanism, and potential intervention are still limited. This study compared venom from TE and NV for their composition, toxicity, and efficacy in vitro and in vivo used RAW264.7 cells and ICR mice. A total of 239 and 225 toxin proteins were identified in TE and NV by proteomics, respectively. Pathological analysis revealed that TE and NV caused heart and liver damage through apoptosis, necrosis, and inflammation, while TE exhibited higher toxicity ex vivo and in vivo. Biochemical markers indicated TE and NV elevated creatine kinase, lactatedehydrogenase, and aspartate aminotransferase, with the TE group showing a more significant increase. Transcriptomics and Western blotting indicated both venoms increased cytokines expression and MAPK signaling pathways. Additionally, 1 mg/kg PACOCF3 (the phospholipase A2 inhibitor) improved survival from 16.7% to 75% in mice. Our results indicate that different extraction methods impact venom activities, tentacle autolysis preserves toxin proteins and their toxicity, and PACOCF3 is a potential antidote, which establishes a good extraction method of jellyfish venom, expands our understanding of jellyfish toxicity, mechanism, and provides a promising intervention.
Low Calcium–High Magnesium Krebs–Henseleit Solution Combined with Adenosine and Lidocaine Improved Rat Aortic Function and Structure Following Cold Preservation
Authors: Arsyad Aryadi , Lembang Geni K. R. , Linda Sesilia L. , Djabir Yulia Y. , Dobson Geoffrey P.
Published: August 2024

Background and objectives: The main problem of vascular preservation is the maintenance of vessel graft quality and function following extended storage. Conventional preservation solutions such as histidine–tryptophan–ketoglutarate (HTK) solution, Phosphate-Buffer Solution (PBS), or sodium chloride 0.9% has been shown to be inadequate in preserving vascular physiological function after 3 days of cold storage. This study aimed to evaluate whether adenosine and lidocaine (AL) in a modified Krebs–Henseleit (KH) solution can preserve the function and histological structure of rat aortic rings after 6 days. Materials and Methods: Thirty-five aortic rings from male Wistar rats (200–300 g) were harvested and immediately immersed in one of the assigned cold preservation solutions: standard KH, modified KH (mod KH) with lower calcium (Ca2+) and higher magnesium content (Mg2+) with or without adenosine and lidocaine (mod KH-AL), and modified KH with AL, insulin, and melatonin (Mod KH-ALMI). The contraction and relaxation function of the aortic rings were examined using an isometric force transducer after 6 days of cold preservation. Hematoxylin and eosin staining were used to analyze the rings’ histological structure. Results: Vascular contraction and relaxation functions were severely affected after a 6-day cold storage period in standard KH. Modifying the KH solution by reducing the Ca2+ and increasing the Mg2+ levels greatly recovered the vessel functions. The addition of AL or ALMI to the modified KH did not further recover vascular contractility. However, only the addition of AL to the modified KH increased the ACh-induced relaxation at 6 days when compared to the conventional KH, suggesting that endothelium preservation is improved. From histological analysis, it was found that the addition of AL but not ALMI further improved the endothelial lining and the structure of the elastic membrane layers of the preserved vessels after 6 days of cold preservation. Conclusions: The addition of AL to low calcium-high magnesium KH solution significantly enhanced endothelial preservation and improved endothelial-induced relaxation of preserved vessels after 6 days of cold storage.
Well-Being of Orthodontic Patients Wearing Orthodontic Appliances
Authors: Nedzinskaitė Rugilė , Augytė Benedikta , Smailiene Dalia , Vasiliauskas Arūnas , Lopatiene Kristina , Zasčiurinskiene Egle , Trakiniene Giedre
Published: August 2024

Background and Objectives: Orthodontic appliances may cause discomfort for patients. This could influence the person’s psychological well-being. The aim of this study was to examine the psychological health of patients wearing orthodontic appliances. It is important to analyze the well-being of patients during orthodontic treatment because it is started in a young age and it may affect one’s psychological health. Therefore, for doctors and patients, it is important to understand and take every aspect of the treatment into consideration when deciding when to start the treatment and what appliance to choose. Materials and Methods: A total of 339 patients filled out an anonymous questionnaire. They were divided into four groups according to their age and type of appliance. The main reasons to seek treatment were crooked teeth and a bad bite. There was a statistically significant difference between women and men as women sought treatment because of crooked teeth; meanwhile, men indicated bad bite as their main reason for seeking treatment. Results: A total of 48.7% of subjects noted that they were feeling good during treatment; however, 4% of all patients noted that they were feeling bad. A total of 45% of subjects stated that they felt unhappy at least sometimes. A total of 43% of subjects felt stress. Conclusions: Even though patients indicated various negative aspects on their well-being during treatment, for the majority of the subjects, the end results significantly increased their psychological well-being.
The Role of Akkermansia muciniphila on Improving Gut and Metabolic Health Modulation: A Meta-Analysis of Preclinical Mouse Model Studies
Authors: Khalili Leila , Park Gwoncheol , Nagpal Ravinder , Salazar Gloria
Published: August 2024

Akkermansia muciniphila (A. muciniphila) and its derivatives, including extracellular vesicles (EVs) and outer membrane proteins, are recognized for enhancing intestinal balance and metabolic health. However, the mechanisms of Akkermansia muciniphila’s action and its effects on the microbiome are not well understood. In this study, we examined the influence of A. muciniphila and its derivatives on gastrointestinal (GI) and metabolic disorders through a meta-analysis of studies conducted on mouse models. A total of 39 eligible studies were identified through targeted searches on PubMed, Web of Science, Science Direct, and Embase until May 2024. A. muciniphila (alive or heat-killed) and its derivatives positively affected systemic and gut inflammation, liver enzyme level, glycemic response, and lipid profiles. The intervention increased the expression of tight-junction proteins in the gut, improving gut permeability in mouse models of GI and metabolic disorders. Regarding body weight, A. muciniphila and its derivatives prevented weight loss in animals with GI disorders while reducing body weight in mice with metabolic disorders. Sub-group analysis indicated that live bacteria had a more substantial effect on most analyzed biomarkers. Gut microbiome analysis using live A. muciniphila identified a co-occurrence cluster, including Desulfovibrio, Family XIII AD3011 group, and Candidatus Saccharimonas. Thus, enhancing the intestinal abundance of A. muciniphila and its gut microbial clusters may provide more robust health benefits for cardiometabolic, and age-related diseases compared with A. muciniphila alone. The mechanistic insight elucidated here will pave the way for further exploration and potential translational applications in human health.
Exploring the Mutated Kinases for Chemoenzymatic Synthesis of N4-Modified Cytidine Monophosphates
Authors: Koplūnaitė Martyna , Butkutė Kamilė , Stankevičiūtė Jonita , Meškys Rolandas
Published: August 2024

Nucleosides, nucleotides, and their analogues are an important class of molecules that are used as substrates in research of enzymes and nucleic acid, or as antiviral and antineoplastic agents. Nucleoside phosphorylation is usually achieved with chemical methods; however, enzymatic phosphorylation is a viable alternative. Here, we present a chemoenzymatic synthesis of modified cytidine monophosphates, where a chemical synthesis of novel N4-modified cytidines is followed by an enzymatic phosphorylation of the nucleosides by nucleoside kinases. To enlarge the substrate scope, multiple mutant variants of Drosophila melanogaster deoxynucleoside kinase (DmdNK) (EC:2.7.1.145) and Bacillus subtilis deoxycytidine kinase (BsdCK) (EC:2.7.1.74) have been created and tested. It has been determined that certain point mutations in the active sites of the kinases alter their substrate specificities noticeably and allow phosphorylation of compounds that had been otherwise not phosphorylated by the wild-type DmdNK or BsdCK.
ZnO–Graphene Oxide Nanocomposite for Paclitaxel Delivery and Enhanced Toxicity in Breast Cancer Cells
Authors: Madeo Lorenzo Francesco , Schirmer Christine , Cirillo Giuseppe , Asha Ayah Nader , Ghunaim Rasha , Froeschke Samuel , Wolf Daniel , Curcio Manuela , Tucci Paola , Iemma Francesca , Büchner Bernd , Hampel Silke , Mertig Michael
Published: August 2024

A ZnO-Graphene oxide nanocomposite (Z-G) was prepared in order to exploit the biomedical features of each component in a single anticancer material. This was achieved by means of an environmentally friendly synthesis, taking place at a low temperature and without the involvement of toxic reagents. The product was physicochemically characterized. The ZnO-to-GO ratio was determined through thermogravimetric analysis, while scanning electron microscopy and transmission electron microscopy were used to provide insight into the morphology of the nanocomposite. Using energy-dispersive X-ray spectroscopy, it was possible to confirm that the graphene flakes were homogeneously coated with ZnO. The crystallite size of the ZnO nanoparticles in the new composite was determined using X-ray powder diffraction. The capacity of Z-G to enhance the toxicity of the anticancer drug Paclitaxel towards breast cancer cells was assessed via a cell viability study, showing the remarkable anticancer activity of the obtained system. Such results support the potential use of Z-G as an anticancer agent in combination with a common chemotherapeutic like Paclitaxel, leading to new chemotherapeutic formulations.
Climate Change and Nitrogen Dynamics: Challenges and Strategies for a Sustainable Future
Authors: Viancelli Aline , Michelon William
Published: August 2024

Global warming driven by climate change has profound impacts on nitrogen dynamics in terrestrial and aquatic ecosystems. The increased emissions of greenhouse gases alter the distribution and availability of nitrogen, which is a critical nutrient for all living organisms. This review examines the connections between climate change and nitrogen cycling, highlighting the adverse effects on ecosystem health and productivity. The proliferation of nitrogen pollution due to agricultural runoff, industrial effluents, and urban wastewater aggravates eutrophication, leading to significant environmental and economic consequences. The imbalance in nitrogen availability not only affects plant growth and soil fertility but also disrupts aquatic ecosystems, resulting in harmful algal blooms and hypoxic conditions. Effective mitigation and adaptation strategies are essential to addressing these challenges. Sustainable agricultural practices, such as precision farming and the use of slow-release fertilizers, along with robust policies and innovative technologies, like biochar application and nitrification inhibitors, are essential in managing nitrogen levels. This review underscores the importance of interdisciplinary approaches that involve integrating insights from ecology, agronomy, and the social sciences to develop comprehensive solutions. Future research should focus on long-term studies to assess the cumulative impacts of climatic changes on nitrogen availability and ecosystem health to guide policies and management practices for sustainable development.
High-Protein Nutritional Supplements Improve Nutritional Status in Malnourished Patients with Systemic Sclerosis
Authors: Wojteczek Anna , Dardzińska Jolanta , Ziętkiewicz Marcin , Smoleńska Żaneta , Czuszyńska Zenobia , De Cock Diederik , Zdrojewski Zbigniew , Małgorzewicz Sylwia , Chmielewski Michał
Published: August 2024

(1) Background: Impaired nutritional status in systemic sclerosis (SSc) is prevalent. (2) Objective: This study aimed to identify pre-cachexia and malnutrition in SSc patients and to estimate the effectiveness of a high-protein oral nutritional supplement (ONS) in improving their nutritional status. (3) Materials and methods: The SSc population comprised 56 patients and a control group of 49 healthy persons. After a baseline clinical evaluation, bioelectrical impedance analysis (BIA), and laboratory tests, SSc patients were divided into well-nourished, pre-cachectic, and malnourished categories. SSc patients with a nutritional disbalance received a high-protein ONS once daily for 3 months. Patients were reassessed at 3 and 12 months after inclusion in the study. (4) Results: SSc patients, in comparison to the control group, had a significantly lower seven-point SGA value [6(0) vs. 7(1), p < 0.001)], lean tissue mass [LTM, 35.1 (10.5) vs. 40.1 (10.10), p = 0.008], and lean tissue index [LTI, 13.5 (3) vs. 14.9 (4), p = 0.009]. Of the 56 SSc patients, 40 (71.4%) were well nourished, 5 (8.9%) had pre-cachexia, and 11 (19.7%) were malnourished. A high-protein ONS in the pre-cachexia group stabilized the SGA value, anthropometric measurements, and BIA after 3 and 12 months. In malnourished patients, it significantly improved the SGA value [5(0) vs. 6(0), p = 0.002], LTI [12.1 (2.1) vs. 12.7 (3.2), p = 0.021] and LTM [31.1 (7.7) vs. 35.1 (9.1), p = 0.021], and that effect remained stable at 12 months. (5) Conclusion: Malnutrition is a common complication of SSc that can be improved with nutritional intervention.