bluuebunny/crossref_metadata_2025 huggingface dataset:
Ni(II) complex based on imidazole dicarboxylic acid as a promising electrocatalyst for hydrogen evolution reaction and H2O2-sensing
Authors: Wan Tiantian , Shen Qinqin , Kong Xiaoxia , Li Kaiyi , Wu Huilu
Published: March 2023

A new mononuclear Ni(II) complex, [Ni(p-MOPhH2IDC)2(H2O)2], was synthesized by the reaction of p-MOPhH3IDC (2-(4-methoxyphenyl)-1H-imidazole-4,5-dicarboxylic acid) and Ni(NO3)2·6H2O under solvothermal conditions and characterized by single-crystal X-ray diffraction, elemental analysis, IR and UV-vis spectroscopy. The structure analysis revealed that the nickel center was six-coordinated octahedron coordination geometry. The electrochemical properties of the Ni(II) complex-doped carbon paste electrode (Ni-CPE) were investigated. For electrocatalytic HER (hydrogen evolution reaction), linear sweep voltammetry curves at different temperatures were determined. The polarization curve shows that the η10298K (overpotential, 10 mA cm–2) of the Ni-CPE was positively shifted by 265 mv compared with the bare-CPE (without complex). The Tafel slope of the Ni-CPE was 187 mV dec-1. These indicated that the Ni-CPE was effective for HER electrocatalytic reaction. In addition, the electrochemical sensing performances of the Ni-CPE towards H2O2 were found to have a linear response from 0.5 μM to 4.0 mM with a detection limit of 0.036 μM. The information obtained from this study will help to expand the application of Ni(II) complex in the field of electrochemistry.
Electrochemical Sensitive and Selective Detection of Vitamin-C: Al2O3@PPy Carbon Nanotubes on Modified GCE
Authors: P Manikanta , Navya Byragondanahalli Suresh , . Mounesh , Nikham Rohith Ranganatha , Nagaraja Bhari Mallanna
Published: March 2023

The dispersion of PPy carbon nanotubes catalysts is crucial for the electro-catalytic ability of ascorbic acid (AA), which can be influenced by the fabrication method and the operation process of the catalysts. Therefore, a fabrication method of co-precipitation, have been applied to prepare Al2O3@PPy carbon nanotubes (Al2O3@PPy/CNTs) catalyst. The fabrication method can partially influence the structural and catalytic activity of the AA. The synthesised Al2O3@PPy /CNTs catalyst was confirmed by various spectroscopic methods and as well as electrocatalytic activity of AA by CVs, DPV, and chrono amperometry technique. The results demonstrate that the co-precipitation method is strongly efficient for fabricating PPy carbon nanotubes-based catalysts with a high stability, selectivity, and reproducibility of Al2O3@PPy/CNTs/GC electrode. The fabrication effect can be associated with its low detection limit (1.25 nM L-1), linear range (50 to 700 µM) and sensitivity (7.943 nM µA cm-2), which is achieved via the hot spot mechanism.
The association of obesity with post-cesarean inpatient opioid consumption
Authors: Strong Abigail , Tvina Alina , Harrison Rachel , Watkins Jayla , Afreen Esah , Tsaih Shirng-Wern , Palatnik Anna
Published: March 2023

Background: Obesity and prescription opioid misuse are important public health concerns in the United States. A common intersection occurs when women with obesity undergo cesarean birth and receive narcotic medications for postpartum pain.
Objective: To examine the association between obesity and inpatient opioid use after cesarean birth.
Methods: A retrospective cohort study of patients that underwent cesarean birth in 2015-2018. Primary outcome was post-cesarean delivery opioid consumption starting 24 hours after delivery measured as morphine milliequivalents per hour (MME/hr). Secondary outcome was MME/hr consumption in the highest quartile of all subjects. Opioid consumption was compared between three BMI groups: non-obese BMI 18.5-29.9 kg/m2; obese BMI 30.0-39.9 kg/m2; and morbidly obese BMI ≥ 40.0 kg/m2 using univariable and multivariable analyses.
Results: Of 1,620 patients meeting inclusion criteria, 496 (30.6%) were in the non-obese group, 753 (46.5%) were in the obese group, and 371 (22.9%) were in the morbidly obese group. In the univariate analysis, patients with obesity and morbid obesity required higher MME/hr than patients in the non-obese group [1.3 MME/hr (IQR 0.1, 2.4) vs. 1.6 MME/hr (IQR 0.5, 2.8) vs. 1.8 MME/hr (IQR 0.8, 2.9), for non-obese, obese, and morbidly obese groups respectively, p<0.001]. In the multivariable analysis, this association did not persist. In contrast, subjects in the obese and morbidly obese groups were more likely to be in the highest quartile of MME/hr opioid consumption compared with those in the non-obese group (23.5% vs. 48.1% vs. 28.4%, p<0.001, respectively); with aOR 1.42 (95% CI 1.07-1.89, p-0.016) and aOR 1.60 (95% CI 1.16-2.22, p=0.005) for patients with obesity and morbid obesity, respectively.
Conclusion: Maternal obesity was not associated with higher hourly MME consumption during inpatient stay after cesarean birth. However, patients with obesity and morbid obesity were significantly more likely to be in the top quartile of MME hourly consumption.
A combined rapid clinical and lung ultrasound score for predicting bronchiolitis severity
Authors: Camporesi Anna , Morello Rosa , Guzzardella Amedeo , Pierucci Ugo Maria , Izzo Francesca , De Rose Cristina , Buonsenso Danilo
Published: March 2023

Introduction
Lung Ultrasound (LUS) has demonstrated good correlation with clinical severity scores in bronchiolitis but should be combined with clinical data to achieve best results. Aim of the study is to create a quick and reliable clinical-ultrasound score to predict the risk of Pediatric Intensive Care (Picu) admission as soon as the patient enters the Emergency Department (ED).
Methods
Retrospective study conducted at two pediatric EDs. Lung was divided into six zones and scanned with ultrasound; every zone received a score. Clinical data were obtained. For the outcomes “Picu admission”and “Cpap ventilation”, a multivariate analysis was conducted and the significant factors resulting were used to create a 3- items score to predict Picu admission. Area under the Receiver-Operating Curve (AUC) for specificity and sensibility of the score was obtained.
Results
74 patients were enrolled; 34% were admitted to PICU. 31% were treated with CPAP. For the outcome “PICU admission”, multivariate analysis demonstrated presence of wheezing and reduced oral intake to be significant together with ultrasound involvement of the Right Posterior Upper Zone and Left Posterior Basal Zone. For the outcome “CPAP ventilation”, same clinical factors plus involvement of Right Posterior Upper Zone were significant. A 3-items score (1: presence of wheezing; 2: reduced oral intake; 3: LUS involvement of Right Posterior Upper Zone) for prediction of PICU admission was created which presents an AUC of 0.8249.
Conclusions
We were able to create a simple and quick score to predict need for PICU admission in bronchiolitis.
RTP801 Mediates Transneuronal Toxicity via Extracellular Vesicles by Affecting Their Protein Cargo and Abrogating Their Trophic Effect
Authors: Solana-Balaguer Júlia , Martín-Flores Núria , Garcia-Segura Pol , Campoy-Campos Genís , Pérez-Sisqués Leticia , Chicote-González Almudena , Fernández-Irigoyen Joaquín , Santamaría Enrique , Pérez-Navarro Esther , Alberch Jordi , Malagelada Cristina
Published: March 2023

Background

Extracellular vesicles (EVs) play a crucial role in intercellular communication, participating in the paracrine trophic support or in the propagation of toxic molecules, including proteins. RTP801 is a stress-regulated protein, whose levels are elevated during neurodegeneration and induce neuron death. However, whether RTP801 toxicity is transferred trans-neuronally via EVs remains unknown.

Methods

We overexpressed or silenced RTP801 protein in cultured cortical neurons and isolated the neural-derived EVs (RTP801-EVs, or shRTP801-EVs respectively). We characterized their protein content by mass spectrometry (MS) and western blotting (WB). RTP801-EVs toxicity was assessed by treating cultured neurons with these EVs and quantifying apoptotic neuron death and branching. We also tested shRTP801-EVs functionality in the pathologic in vitro model of 6-Hydroxydopamine (6-OHDA) by biochemical analyses.

Results

Expression of RTP801 increased the number of EVs released by neurons. Moreover, RTP801 led to a distinct proteomic signature of neural-derived EVs, containing more pro-apoptotic markers. Hence, we observed that RTP801 toxicity was transferred to neurons via EVs, activating apoptosis and impairing neuron morphology complexity. In contrast, EVs derived from neurons where RTP801 was silenced were able to increase the arborization of recipient neurons. We also showed that 6-OHDA neurotoxin elevated protein levels of RTP801 in both cell lysates and EVs. Furthermore, neural-derived EVs induced phosphorylation of Ser473-Akt and Ser235/236-RPS6 in recipient neurons, and this effect was lost when EVs were derived from neurons treated with 6-OHDA. Interestingly, EVs derived from neurons where RTP801 was silenced prior to exposing them to 6-OHDA maintained Akt and RPS6 transactivation in recipient neurons.

Conclusion

Taken together, these results suggest that RTP801 toxicity can be spread via EVs and therefore, it could contribute to the progression of neurodegenerative diseases in which RTP801 is involved.
Prognosis Evaluation of Acute Ischemic Stroke After Endovascular Treatment: A Synchronous Study of Transcranial Doppler and Quantitative Electroencephalography
Authors: Qi Yajie , Xing Yingqi , Wang Qingduo , Cao Yanting , Chen Hongxiu , Chen Ying
Published: March 2023

Background: Patients with acute ischemic stroke (AIS) caused by large vessel occlusions who are successfully recanalized may still have a poor prognosis. Transcranial Doppler (TCD) and quantitative electroencephalography (QEEG) were used to assess cerebral blood flow and metabolism, with the goal of identifying patients with severe neurological deficits and poor prognosis at an early stage.
Methods: We prospectively recruited AIS patients with large vessel occlusions with successful recanalization after endovascular treatment and assessed their prognosis at 90 days using the modified Rankin Scale (mRS) score. All patients’ clinical Information, Glasgow Coma Scale (GCS), and National Institute of Health Stroke Scale (NIHSS) scores were recorded. TCD-QEEG was used to evaluate the brain functioning of AIS patients.
Results:Thirty-seven patients (63±11.7 years; 24 males) were studied, and 18 patients had a poor prognosis at 90 days which mRS >3. Multivariable analyses showed that TCD indicators ofUPI (pulsatility index of unaffected side) (odds ratio [OR] 1.076, 95% confidence interval [CI] 1.001–1.157, p=0.048), QEEG indicators of pairwise derived brain symmetry index (pdBSI) (OR 3.041, 95%CI 1.040–8.897, p=0.042), and NIHSS score (OR 1.638, 95%CI 1.071–2.504, p=0.023) were independent predictors of clinical outcomes. The area under the ROC curve for the model combining UPI, pdBSI, and NIHSS scores was 0.980, demonstrating superior predictive accuracy than any single variable.
Conclusions: The combination of TCD, QEEG, and NIHSS scores demonstrated the best prognostic assessment accuracy by assessing cerebral blood flow, metabolism, and neurological function in patients with AIS after revascularization.
Using a Privacy-Enhanced Authentication Process to Secure Internet of Things Infrastructures
Authors: Rostampour Samad , Bagheri Nasour , Ghavami Behnam , Bendavid Ygal , Kumari Saru , Martin Honorio , Camara Carmen
Published: March 2023

Over the last decade, technological advances in smart grids have permitted the modernization of legacy electricity networks. As Internet of Things(IoT)-based smart grids are becoming an efficient response to managing changing electric demand, the heterogeneous network of equipment required to make these Cyber-Physical Systems a reality poses some security threats. This paper proposes a novel mutual authentication and key agreement scheme to ensure communications security and protect users' privacy in smart grid applications. In the proposed scheme (named EPSG), an Elliptic Curve Cryptography (ECC) module and a Physical Unclonable Function (PUF) are used simultaneously to provide acceptable confidentiality and integrity levels. The security analysis demonstrates that the EPSG has a robust security posture regarding transferred messages on the communication channel and physical attacks. In addition, EPSG is resistant to modeling attacks as one of the main vulnerabilities of PUF modules. Furthermore, by implementing the EPSG on an Arduino UNO microcontroller, a comparative performance evaluation in terms of computational complexity, communication overhead, and power consumption demonstrates the efficiency of the proposed EPSG.
Reproducible Clustering with Non-Euclidean Distances: A Simulation and Case Study
Authors: Staples Lauren , Ring Janelle , Fontana Scott , Ray Herman , Zhang Yifan , Zhang Xinyan , Stradwick Christina
Published: March 2023

Certain categorical sequence clustering applications require path connectivity, such as the clustering of DNA, click-paths through web-user sessions, or paths of care clustering with sequences of patient medical billing codes. K-means and k-medoids clustering with non-Euclidean distance metrics such as the Jaccard or edit distances maintains such path connectivity. Although k-means and k-medoids clustering with the Jaccard and edit distances have enjoyed success in these domains, the limits of accurate cluster recovery in these conditions have not yet been defined. As a first step in approaching this goal, we performed a simulated study using k-means and k-medoids clustering with non-Euclidean distances and show the performance deteriorates at a certain level of noise and when the number of clusters increases. However, we identify initialization strategies that improve upon cluster recovery in the presence of noise. We employ the use of the Tibshirani and Guenther (2005) Prediction Strength method, which creates a hypothesis testing scenario that determines if there is clustering structure to the data (if the clusters are reproducible), with the null hypothesis being there is none. We then applied the framework to perinatal episodes of care and the clusters reproducibly and organically split between Cesarean and vaginal deliveries, which itself is not a clinical finding but sensibly validates the approach. Further visualizations of the clusters did bring insights into subclusters that split along groups of physicians, cost and risk scores, warranting the outlined future work into ways of improving this framework for better resolution.
The long noncoding RNA uc003pxg.1 interacts with miR-339-5p to promote TGF-β1 expression and vascular endothelial cell proliferation, migration and angiogenesis in coronary heart disease
Authors: Li Ping , Sun Chenchen , Yan Xinxin , Huang Ying , Xu Jingyi , Wang Feng , Weng Jiayi , Li Yuan , Sun Kangyun
Published: March 2023

This study aims to investigate the roles of lncRNA uc003pxg.1 and miR-339-5p in regulating the proliferation, migration and angiogenesis of vascular endothelial cells and affect the occurrence and development of coronary heart disease (CHD). First, the expression levels of uc003pxg.1 and miR-339-5p were verified in clinical samples, and the correlation between them was analyzed. Then, the target gene was identified using high-throughput sequencing combined with bioinformatics. Human umbilical vein endothelial cells (HUVECs) were transfected with si-uc003pxg.1, miR-339-5p mimic and miR-339-5p inhibitor, and the expression of related genes was detected by RT-qPCR. EdU, CCK8 and Transwell assays were used to analyze the effects of uc003pxg.1 and miR-339-5p on cell proliferation and migration. The protein expression levels were detected by western blotting and angiogenesis was observed. The expression of uc003pxg.1 and miR-339-5p was negatively correlated in clinical samples and HUVECs. si-uc003pxg.1 and miR-339-5p mimic decreased the proliferation and migration of HUVECs and decreased the expression of TGF-β1 and α-SMA. The protein expression levels of TGF-β1, α-SMA, CD31, collage I, collage III and endoglin were decreased, and angiogenesis was weakened. The miR-339-5p inhibitor had the opposite effect. Our study revealed that upregulation of uc003pxg.1 and downregulation of miR-339-5p in vivo promotes cell proliferation, cell migration and angiogenesis, and up-regulates the expression of TGF-β1, α-SMA, CD31, collage I, collage III and endoglin, which may lead to the development of vascular atherosclerosis.
Benign Enlargement of Subarachnoid Spaces in Infancy with Developmental Delay
Authors: Roy Joanna M. , Sawant Vishal Dnyaneshwar , Yadav Jayesh
Published: March 2023

Background
Benign enlargement of subarachnoid spaces (BESS) is a common cause of macro crania in infancy and may present with developmental delay due to enlargement of the brain. The self-limiting nature of this condition allows most children to regain developmental milestones as they grow older. The aetiology of developmental delay in full-term infants with BESS is unclear and prognosis varies since only some children have been reported to have long-term deficit in gross motor and language domains.
Case presentation:
This case report illustrates the clinical profile and findings on brain imaging in a full-term infant who presented with BESS along with delay in attaining age-appropriate gross motor and language milestones. Assessment of developmental milestones at 15 months of age supported the benign nature of this condition although the head circumference remained above the 95th percentile for age.
Conclusions
Despite the self-resolving nature of this condition, regular evaluation of developmental milestones is necessary to ensure timely intervention in appropriate cases. Further research on the aetiology could also aid clinicians in determining which children are at risk for developing long-term developmental delay.
Nonlinear deformation effect of unloading rate on hard rock under different confining pressures
Authors: Jia Lexin , Cong Yu , Qiu Shili , Wang Xiaoshan , Zhang Shirui
Published: March 2023

In this paper, triaxial loading and unloading tests under different loading and unloading conditions are carried out to explore the deformation and failure characteristics of hard rock marble, and the acoustic emission (AE) information of the test process is monitored synchronously to obtain the AE distribution characteristics. The test results show that: (1) when the unloading rate of confining pressure is small, the yield of rock is more obvious, the failure peak value is higher, and the Poisson effect is more obvious under loading and unloading, while when the unloading rate is larger, it shows multi-stage failure; (2) there is a linear relationship between strain deflection stress compliance and unloading rate. With the increase of unloading rate, the circumferential and volumetric compliance increases and the axial compliance decreases; (3) the smaller the unloading rate is, the greater the confining pressure is, and a large number of tensile microcracks in the main shear plane of rock form a shear zone, while the higher the unloading rate is, the smaller the confining pressure is, and the main shear plane runs through; (4) AE counts can characterize the damage degree of marble during loading and unloading. When the confining pressure is low, the damage peak of AE signals increases sharply, and the overall damage increases with the increase of unloading rate, and when the confining pressure is high, AE signals increases gradually. The overall damage decreases with the increase of unloading rate, and a “pre-peak to post-peak” lag effect can occur in the general rock failure.
Genotyping Investigation of Ciprofloxacin Resistance Genes (Parc And Pare) In Clinical Pseudomonas Aeruginosa Isolates
Authors: Dukhan Noor , Ghareeb Abdulameer , Zedan Tamara
Published: March 2023

Purpose
Due to Pseudomonas aeruginosa's low susceptibility to the development of antibiotic resistance and antimicrobial drugs, therapy and control of severe infections often result in recurrent difficulties. A bacterial isolate with multidrug resistance (MDR) is one that is resistant to at least one compound from more than three antimicrobial groups, such as aminoglycosides and fluoroquinolones (10). Human burn wounds are complex environments where necrotic tissue and exudates formed from plasma develop.
Methods
The samples were immediately cultivated on Pseudomonas aeruginosa-selective Blood, MacConkey, Nutrient, and Cetrimide agar media and incubated overnight at 37°C under aerobic conditions. Several biochemical tests, including Oxidase and Catalase tests, were used to identify P. aeruginosa. Additionally, the identification was confirmed using a quick identification procedure (VITEK 2 System). After adding 20% glycerol, the detected isolates were kept at -20°C.
Results
The findings of the local isolates' sequences being aligned with the reference strain revealed some mismatches in the nucleotides of our query at various locations, including deletions and replacements. According to the findings in table 4, there were discrepancies between the isolates from burn that were resistant to ciprofloxacin (P1 and P16) and the reference bacteria (CP034435).
Conclusion
The following are the findings of the current study: Pseudomonas aeruginosa is more prevalent in burn samples than in other clinical samples. Misuse of these antibiotics may contribute to an increase in MDR Pseudomonas aeruginosa, particularly resistance to fluroquinolones (Ciprofloxacin).
Extended-spectrum β-lactamase- producing gram-negative bacterial infections in severely ill COVID-19 patients admitted in a national referral hospital, Kenya
Authors: Mutua Jeniffer Munyiva , Mwaniki John Njeru , Musyoki Abednego Moki
Published: March 2023

Background: Bacterial infections in COVID-19 patients, especially those caused by multidrug-resistant gram-negative strains, are associated with increased morbidity, hospital stay and mortality. However, there is limited data on the epidemiology of extended-spectrum β-lactamase (ESBL)-producing bacteria in COVID-19 patients. Here, we assessed the prevalence and the factors associated with ESBL-producing gram-negative bacteria (GNB) infections among severely ill laboratory-confirmed COVID-19 patients admitted at Kenyatta National Hospital (KNH), Kenya.
Methods: We adopted a descriptive cross-sectional study design for patients admitted between October 2021 and February 2022, purposively recruiting 120 participants based on clinical presentation. Demographics and clinical characteristics data were collected using structured questionnaires and case report forms. Clinical samples were collected and analyzed by standard microbiological methods in the KNH Microbiology laboratory and the Centre for Microbiology, Kenya Medical Research Institute.
Results: GNB infections prevalence was 40.8%, with the majority caused by ESBL – producers (67.3%) predominated by Klebsiella pneumoniae (45.5%). Generally, 73% of the ESBL producers harboured our target ESBL genes, mainly CTX-M-type (59%, 17/29) in K. pneumoniae (76.9%, 20/26). GNB harbouring TEM-type (83%, 10/12) and SHV-type (100%, 7/7) genes showed ESBLs phenotypes and inhibitor resistance, mainly involving clavulanate, but most of them remained susceptible to tazobactam (60%, 6/10). SHV-type genes carrying ESBL producers showed resistance to both cefotaxime CTX) and ceftazidime (CAZ) (K. pneumoniae), CAZ (E. coli) or CTX (E. cloacae complex and K. pneumoniae). About 87% (20/23) of isolates encoding CTX-M-type β-lactamases displayed the typical CTX/ceftriaxone (CRO) resistance phenotype. About 42% of isolates with CTX-M-type β-lactamases only hydrolyzed ceftazidime (CAZ). Isolates with OXA-type β-lactamases were resistant to CTX, CAZ, CRO, cefepime and aztreonam. Patients with comorbidities were about ten (10) times more likely to have an ESB-producing GNB infection (aOR =9.86, 95%CI: 1.30 – 74.63, p =0.003).
Conclusion: We report a high prevalence of ESBL-GNB infections in severely ill COVID-19 patients, predominantly due to Klebsiella pneumoniae harbouring CTX-M type ESBL genes. The patient’s underlying comorbidities increased the risk of ESBL-producing GNB infection. Enhanced systematic and continuous surveillance of ESBL-producing GNB, strict adherence to infection control measures and antimicrobial stewardship policies in the current study setting are warranted.
Crime Prediction Based on Person-Weapons Relation using Deep Learning Techniques
Authors: Ruprah Taranpreet , Shrivastav Hemang
Published: March 2023

Crime Scene detection or predicting the chances of happening the crime without any involvement of human intervention is always the crucial task in the field of artificial intelligence. In this paper crime forecasting based on the weapon detection and tracking with the person can help investigator to understand the sequence of action took place during the crime. The images are manually annotated, which is a process where an expert goes through each images and mark the position and class of object within the image. Object detection and classification algorithms provides the necessary ground to verify data for the algorithms. The models like SSD, YOLO and Faster RCNN are used for weapons detection and mediapipe library is used to generate the human body datapoints and calculate the relation between weapons with the human. The maximum accuracy of Faster RCNN with mediapipe library is 97%.
N1-methyladenosine methylation in mRNAs drives bladder cancer progression
Authors: Song Dongkui , Yin Jianjian , Fan Xin , Chang Qi , Dai Yuanheng , Wang Tao , Shi Lei , Yang Linlin , Yang Xiaoming , Liu Tao , Zhang Xu Dong , Jin Lei , Lu Yong-jie , Zhang Lirong
Published: March 2023

With the growing recognition of RNA modification as a hallmark of cancer, N1-methyladenosine (m1A) methylation has been reported as a key mechanism of post-transcriptional regulation. However, the molecular mechanisms underlying m1A modification in bladder cancer (BLCA) progression remain unclear. In the current study, we aimed to explore the role of m1A methylation in BLCA. We found that the expression of the m1A methyltransferase TRMT61A was significantly elevated in human BLCA tissues. TRMT61A inhibition attenuated BLCA cell proliferation, migration, and invasion in vitro and tumor growth in vivo. Mechanistically, transcriptional profiling identified heme oxygenase-2 (HMOX2) as an m1A modification target of TRMT61A, and HMOX2 mRNA m1A modifications were reduced in TRMT61A-deficient cells. TRMT61A promoted HMOX2 mRNA stabilization in a YTHDF1-dependent manner, and YTHDF1 knockdown decreased the stability of HMOX2 mRNA through an m1A modification-dependent mechanism, leading to the inhibition of tumor cell proliferation. Moreover, NF-κB was found to bind to the promoter region of TRMT61A and stimulate its expression. NF-κB activation also increased the nuclear translocation of TRMT61A. Together, our results demonstrate the oncogenic role of TRMT61A and the m1A modification-mediated NF-κB/TRMT61A/HMOX2 signaling pathway activation in BLCA, thus highlighting a novel therapeutic target for this disease.
Candidicidal Effect of Arginine-based Surfactants
Authors: Fait María Elisa , Grillo Patricia , Garrote Graciela L. , Prieto Eduardo , Vázquez Romina F. , Saparrat Mario C. N. , Morcelle Susana R.
Published: March 2023

Amino-acid–based surfactants are a group of compounds that resemble natural amphiphiles and thus are expected to have a low impact on the environment, owing to either the mode of surfactant production or its means of disposal. Within this context, arginine-based tensioactives have gained particular interest since their cationic nature—in combination with their amphiphilic character—enables them to act as broad-spectrum biocides. This capability is based mainly on their interactive affinity for the microbial envelope that alters the latter’s structure and ultimately its function. In the work reported here, we investigated the efficiency of Nα-benzoyl arginine decyl- and dodecylamide against Candida spp. to further our understanding of the antifungal mechanism involved. For the assays, both a Candida albicans and a Candida tropicalis clinical isolates along with a C. albicans–collection strain were used as references. As expected, both arginine-based compounds proved to be effective against the strains tested through inhibiting both the planktonic and the sessile growth. Furthermore, atomic-force–microscopy techniques and lipid-monolayer experiments enabled us to gain insight into the effect of the surfactant on the cellular envelope. The results demonstrated that all the yeasts treated exhibited changes in their exomorphologic structure, with respect to alterations in both roughness and stiffness, relative to the nontreated ones. This finding—in addition to the amphiphiles’ proven ability to insert themselves within this model fungal membrane—could explain the changes in the yeast-membrane permeability that could be linked to viability loss and mixed-vesicle release.
Perforated voluminous duodenal Burkitt lymphoma causing severe peritonitis, a case report
Authors: jbilou aymane , Aggouri Younes , Ahallat Anass , Aitlaalim Said
Published: March 2023

The management of duodenal malignant lymphoma presents challenges due to the presence of serious complications like obstruction, pancreatitis, and perforation. As a result, a combination of surgical measures and interdisciplinary procedures is necessary. We report a case of a 44 years old man suffering from abdominal pain and vomiting, CT images showed a voluminous intraperitoneal perforated tumour, intra operative exlopration showed that the origin of the tumour is duodenum , a resection was performed , patient died few hours after surgery, , histological and immunohistochemical findings confirmed the diagnostic of burkitt lymphoma . the primary treatment for gastrointestinal lymphomas remains chemotherapy, but in cases of duodenal lymphoma, a significant number of patients need surgery due to complicated presentations.
Disease trajectories in elders with suspected non-Alzheimer’s pathophysiology and its comparison with Alzheimer’s disease pathophysiology: a longitudinal study
Authors: Li Jie-Qiong , Song Jing-Hui , Suckling John , Wang Yan-Jiang , Zuo Chuan-Tao , Zhang Can , Gao Jing- , Song Yu-Qiang , Xie An-Mu , Tan Lan , Yu Jin-Tai
Published: March 2023

Background
According to the new ‘AT(N)’ system, those with a normal amyloid biomarker but with abnormal tauopathy or biomarkers of neurodegeneration or neuronal injury, have been labeled suspected non-Alzheimer’s pathophysiology (SNAP). We aimed to estimate the long-term clinical and cognitive trajectories of SNAP individuals in non-demented elders and its comparison with individual in the Alzheimer’s disease (AD) pathophysiology using ‘AT(N)’ system.
Methods
We included individuals with available baseline cerebrospinal fluid (CSF) Aβ (A), CSF phosphorylated tau examination (T) and 18F-fluorodeoxyglucose PET or volumetric magnetic resonance imaging (N) from the Alzheimer's Disease Neuroimaging Initiative database. Longitudinal change in clinical outcomes are assessed using linear mixed effects models. Conversion risk from cognitively normal (CN) to cognitively impairment, and conversion from mild cognitive impairment (MCI) to dementia are assessed using multivariate Cox proportional hazard models.
Results
Totally, 366 SNAP individuals were included (114 A-T-N-, 154 A-T + N-, 54 A-T-N + and 44 A-T + N+) of whom 178 were CN and 188 were MCI. Compared with A-T-N-, CN elders with A-T + N-, A-T-N + and A-T + N + had a faster rate of ADNI-MEM score decline. Moreover, CN older individuals with A-T + N + also had a faster rate of decline in ADNI-MEM score than those with A-T + N- individuals. MCI patients with A-T + N + had a faster rate of ADNI-MEM and ADNI-EF decline and hippocampal volume loss compared with A-T-N- and A-T + N- profiles. CN older individuals with A-T + N + had an increased risk of conversion to cognitive impairment (CDR-GS ≥ 0.5) compared with A-T + N- and A-T-N-. In MCI patients, A-T + N + also had an increased risk of conversion to dementia compared with A-T + N- and A-T-N-. Compared with A-T + N-, CN elders and MCI patients with A + T + N- and A + T + N + had a faster rate of ADNI-MEM score, ADNI-EF score decline, and hippocampal volume loss. CN individuals with A + T + N + had a faster rate of ADNI-EF score decline compare with A-T + N + individuals. Moreover, MCI patients with A + T + N + also had a faster rate of decline in ADNI-MEM score, ADNI-EF score and hippocampal volume loss than those with A-T + N + individuals.
Conclusions
The findings from clinical, imaging and biomarker studies on SNAP, and its comparison with AD pathophysiology offered an important foundation for future studies.
Application of traditional Chinese medicine in inhibiting the PD-L1 pathway for gastric cancer and colon cancer treatment
Authors: Zhu Xinghui , XIE Li , CHEN DongMei , ZHAO Ming Rui , Liu XiaoMing , FAN ZhiChao , CHENG Ci-Song
Published: March 2023

Programmed death receptor-1 (PD-1) is a suppressor-like receptor located on T cells that interacts with programmed death ligand-1 (PD-L1) to suppress T cell expression, resulting in tumor immune escape. The way to achieve immunotherapy by inhibiting PD-1 and PD-L1 pathways and blocking PD-1 and PD-L1 binding, thereby achieving T-cell upregulation, has been validated by the FDA to enter clinical practice. To date, natural components from herbal medicine such as paclitaxel and camptothecin have been shown to play a role in oncological treatment either alone or in combination with currently known therapies. Meanwhile, many animal experimental studies have shown that natural ingredients from traditional Chinese medicine or traditional Chinese herbal compounds can play a positive role in gastric and colon cancer by inhibiting the PD-L1 pathway. This review focuses on the mechanism of PD-L1 and the modulation of PD-L1 pathway by some traditional Chinese medicines in two common types of digestive cancers, gastric cancer and rectal colon cancer, with the aim to open up new ideas for the development of novel drugs against digestive cancers.
Nucleophosmin 1 cooperates with the methyltransferase DOT1L to regulate H3K79me2 levels and DNA satellites expression at peri-nucleolar heterochromatin
Authors: izzo annalisa , akol ipek , Villarreal Alejandro , Garcia-Miralles Marta , Bovio Patrick , Heidrich Stefanie , Vogel Tanja
Published: March 2023

Background
The histone methyltransferase DOT1L catalyzes methylation of H3K79 and it is highly conserved in mammals. DOT1L plays a functional role in several biological processes including cell cycle regulation, DNA repair, RNA splicing and gene expression, suggesting a complex role in chromatin organization and regulation. Such a remarkable range of functions performed by DOT1L can be the result, at least partially, of its interaction with a plethora of proteins and presence in different complexes.
Results
Here, we characterized the cooperation of DOT1L with the nucleolar protein NPM1 and the impact of both proteins on peri-nucleolar heterochromatin activity. We show that i) DOT1L interacts preferentially with monomeric NPM1 in the nucleus; ii) DOT1L acts in concert with NPM1 to maintain each other’s protein homeostasis; iii) NPM1 depletion results in H3K79me2 upregulation at chromatin remodeling genes but does not affect their expression; iv) DOT1L and NPM1 preserved DNA satellite expression at peri-nucleolar heterochromatin via epigenetic mechanisms dependent on H3K27me3.
Conclusions
Our findings give insights into molecular mechanisms employed by DOT1L and NPM1 to regulate heterochromatin activities around the nucleoli and shed light on one aspect of the complex role of both proteins in chromatin dynamics.
Investigation of Cataract Hardness Within the Scope of Surgical Success and Complication
Authors: Sever Tomurcuk Harbigil , Yarımağa İffet
Published: March 2023

Purpose
Cataract is a disease that affects the quality of vision at almost every stage of life. Traumatic cataract cases are also frequently encountered in addition to cataract cases that develop due to age and genetic factors. In terms of surgery, cataract cases can be divided into hard and soft. How does the hard or soft cataract affect the surgical success during phacoemulsification used for the treatment of cataract? Is cataract classification related to complications? What is the relationship between phaco time and cataract classes? In this study, answers to the above questions were sought.
Materials and methods
A total of 287 eyes of 287 patients who met the conditions for cataract surgery at the Van Training and Research Hospital Eye Clinic between 2020 and 2022 were included in the study. Ethics committee approval was also obtained from the ethics committee of the same hospital. The research design can be described as correlational research. Linear relationships between two or more variables will be tested. Frequency analysis, t test, correlation analysis and regression analysis were applied by means of SPSS (ver. 20) package program.
Results
According to the results of this study, a significant relationship was found between cataract hardness and phaco time. The greater the hardness of the cataract, the longer the phaco period. The prolongation of the phaco period in surgery increases the risk of complications. Contrary to the common results in the literature, posterior capsule rupture complications were more common in soft cataracts in this study. When visual acuity was compared, postoperative visual acuity did not differ between hard and soft cataracts. However, a significant difference was found in the evaluation of the groups within themselves before and after surgery.
Conclusion
Rupture of the posterior capsule is more common in soft cataracts. There is no difference between soft and hard cataracts in terms of visual acuity. In addition, there was no difference between hard and soft cataracts in terms of intraocular pressure.
Effect of light stress on maximum photochemical efficiency of photosystem II and chloroplast structure in cryptogams Cladonia mitis and Pleurozium schreberi
Authors: Dziurowicz Patrycja , Fałowska Patrycja , Waszkiewicz Karolina , Wietrzyk-Pełka Paulina , Węgrzyn Michał H.
Published: March 2023

Earlier beliefs suggested that lichens thrived in open and warm environments, whereas mosses prefer shaded places with high humidity. However, excessive light can be a stress factor for both lichens and bryophytes, as it can damage their photosynthetic apparatus, particularly the chlorophyll pigments located in the chloroplasts. The measurement of photosynthetic activity and the use of fluorescence microscopy allows the assessment of the influence of light as a stress factor on the state of preservation of the thallus. The main goal of our research was to determine the changes that occur in the photosynthetic activity and structure of chloroplasts of selected species lichen Cladonia mitis (Sandst.) and moss Pleurozium schreberi (Willd. Ex Brid.) under the influence of different intensity of light in laboratory conditions: FL − 100% light, RL − 30% maximum light, and NL - natural sunlight. The results of the two-factor analysis of variance (ANOVA) tests showed a variation in the activities of the selected species over time depending on the amount of light energy supplied. The results showed that Cladonia mitis showed the lowest level of chloroplast fluorescence in the strongest and reduced light, which coincides with its low level of photosynthetic activity. The moss showed high and intense fluorescent activity of the chloroplasts, indicating their much higher resistance to light radiation.
Digitalisierung als Störung?
Authors: Wagner Elke , Nord Ilona , Adam Oliver
Published: March 2023

Asymmetrische Praktiken der schulischen Kommunikation werden durch den Einsatz etwa von Tablets, die digitales Lehrmaterial beinhalten, neu strukturiert. In unserer empirisch-ethnografischen Studie von digital vermitteltem Religionsunterricht via Tablets werden Episoden sichtbar, in denen die Schüler:innenschaft zumindest zeitweise zur Lehrkraft symmetrisiert wird. Lehrpersonen reagieren hierauf mit einer veränderten Selbstbeschreibung von Rollenkonstellationen zwischen sich sowie den Schüler:innen. Die Umsetzung der digitalen Unterrichtssituation verläuft allerdings nicht reibungslos: Dabei zeigt sich, dass die herkömmliche Unterrichtssituation derart strukturiert ist, dass sie den Einsatz digitaler Medien zunächst eher behindert als befördert. Wir kommen deshalb in unserer empirisch-qualitativen Analyse auch zu dem Schluss: Es ist der (asymmetrisch organisierte) Unterricht, der den reibungsfreien Vollzug digitaler Medien stört.
Turistlerin Gastronomik Akımlara Yönelik Tüketim Eğilimleri ile Destinasyon Memnuniyetleri Arasındaki İlişki: Kapadokya Örneği (The Relationship Between Tourists Consumption Tendencies Towards Gastronomic Trends and Destination Satisfaction: Case of Cappadocia)
Authors: ÇEŞMECİ Nihat , ŞENER Büşra , KILIÇHAN Reha
Published: March 2023

Bu araştırma, turistlerin gastronomik akımlara yönelik tüketim eğilimleri ile destinasyon memnuniyetleri arasındaki ilişkiyi incelemek üzere gerçekleştirilmiştir. Literatürde modernist, füzyon ve yerel mutfak akımlarına yönelik tüketim eğilimiyle destinasyon memnuniyeti arasındaki ilişkiyi inceleyen çalışmaya rastlanmamasından dolayı, çalışmanın literatüre katkı sağlayacağı düşünülmektedir. Araştırma verileri 2022 yılı Kasım-Aralık ayları içerisinde 313 turistten toplanmıştır. Yapılan korelasyon analizi sonucunda, turistlerin modernist mutfak ve yerel mutfak akımlarına yönelik tüketim eğilimi ile destinasyon memnuniyeti arasındaki ilişkinin orta düzeyde ve olumlu yönde olduğu bulgusuna ulaşılmıştır. Regresyon analizi bulguları incelendiğinde ise destinasyon memnuniyetindeki toplam değişmenin %26,2'sinin modernist mutfak, %11,6’sının füzyon mutfak ve %25,5’inin yerel mutfak tüketim eğilimi ile açıklanabileceği bulgulanmıştır. Gerçekleştirilen t-testi sonucunda, yerli ve yabancı turistler arasında ve ilk defa destinasyona gelenlerle daha önce gelmiş olanlar arasında mutfak akımlarına yönelik tüketim eğilimleri ile destinasyon memnuniyetleri arasında fark olduğu belirlenmiştir. ANOVA sonuçları, modernist mutfak tüketim eğiliminin katılımcıların eğitim düzeylerine göre; yerel mutfak tüketim eğilimlerinin katılımcıların gelir gruplarına göre ve destinasyon memnuniyetinin ise ziyaretin amacına göre farklılık gösterdiğine işaret etmektedir.
Türkiye deki Uçak Temalı Restoranlara Yönelik E-Şikâyetlerin Değerlendirilmesi (Evaluation of E-Complaints for Airplane Themed Restaurants in Turkey)
Authors: GÜRCÜ Emel , DEMİRKOL Şehnaz
Published: March 2023

Bu araştırmanın amacı, Türkiye’de faaliyet gösteren uçak temalı restoranlara yönelik çevrim içi şikâyet unsurlarının belirlenmesidir. Yapılan yerli literatür araştırmasında uçak temalı restoranlarda müşteri şikâyetleri konusunda bir çalışmaya rastlanmamıştır. Bu amaçla veriler, dünyanın en büyük seyahat tavsiye sitelerinden biri olan tripadvisor’dan elde edilmiştir. Elde edilen veriler nitel araştırma yöntemlerinden içerik analizi tekniği ile incelenmiştir. Örneklem beş adet uçak temalı restorandan oluşmakta olup bir restorana ait yoruma ulaşılamamıştır. Araştırmada şikâyet yönetiminin yiyecek içecek işletmeleri üzerindeki önemine değinilerek, müşterilerin en çok hangi konular hakkında şikâyette bulunduğu incelenmiştir. Yorum yapılan dört restoran için toplam 131 yorum incelenmiştir. Müşteri yorumları yiyecek kalitesi, menü çeşitliliği, temizlik, fiyat, atmosfer, çalışanların profesyonel görünümü, restoranın yeri, yemek bekleme süresi ve tema olarak 9 başlıkta kategorize edilmiştir. Buna göre; %28,5 oran ile en çok şikâyet edilen unsur fiyattır. İkinci şikâyet edilen unsur ise %27,7 ile yiyecek kalitesidir. Üçüncü olarak % 12,3 oran ile servis hızı şikâyet edilmektedir.
Yerel Halkın Ekoturizm Algılarının Demografik Özelliklere Göre İncelenmesi: Amasya İli Örneği (Examination of Local People s Perceptions of Ecotourism in Accordance with Demographic Characteristics: Case of Amasya)
Authors: Gökhan KILIÇ Ahmet , GENÇER Kansu
Published: March 2023

Bu araştırmanın amacı, Amasya yerel halkının ekoturizm algılarının demografik özelliklerine göre anlamlı farklılık gösterip göstermediğini belirlemektir. Amasya’da yaşayan 440 kişiye uygulanan anket formu sonucunda toplanan veriler Bağımsız Örneklemler T Testi ve Tek Yönlü Varyans Analizi (ANOVA) testi aracılığı ile istatistiksel olarak analiz edilmiştir. Analizlerde ekoturizm algılarının, Amasya’da yaşayan yerel halkın cinsiyet, medeni durum, yaş, eğitim durumu, memleket, turizm eğitimi alma durumu, meslek ve ikamet süresine göre istatistiksel olarak farklılaşma durumları değerlendirilmiştir. Buna göre ekoturizm algılarının yalnızca turizm eğitimi alma durumuna göre farklılık gösterdiği, diğer demografik özelliklere göre ise istatistiksel olarak anlamlı bir farklılık göstermediği bulgularına ulaşılmıştır. Bununla birlikte turizm eğitimi alanlar ve almayanların ekoturizme yönelik algılarında farklılaşma gözlemlense de iki kesimin de ekoturizme yönelik algılarının beşli likert ölçeğine göre orta düzeyin üzerinde bir puan olması dikkat çekicidir. Turizm eğitimi almayan katılımcıların algıları ekoturizme yönelik olarak daha olumlu düzeyde bulunmuştur. Bu durumun, ağırlıklı olarak kitlesel turizme yönelik hizmet sunan Türkiye açısından turizm eğitimlerinin ekoturizmin önemini vurgulamakta yetersiz kalması, Amasya bölgesinin ekoturizm odaklı istihdam olanaklarının az olması, bölgenin turizm tanıtım faaliyetlerinin ekoturizmden daha çok kültür turizmine yönelik gerçekleştiriliyor olması nedenlerinden kaynaklandığı düşünülmektedir.
Türkiye de Sektörel Bazda Turizm ve Kadın İstihdamı İlişkisi (The Relationship between Tourism and Women s Employment on Sectoral Basis in Turkey)
Authors: TÜRKMEN Adem , YARBAŞI İkram Yusuf
Published: March 2023

Gerek büyüme gerek de kalkınma açısından büyük bir öneme sahip olan kadın istihdamı, ekonomik açıdan kadın güçlendirilmesinin önemli bir parçasını teşkil etmektedir. Bu noktada turizm gelişimi, sektörün içinde ve diğer sektörlerde kadın istihdamını etkileme bakımından ciddi bir potansiyele sahiptir. Bu araştırma ile Türkiye’de turizm gelişiminin tarım, sanayi ve hizmetler sektöründeki kadın istihdamını nasıl etkilediği, ARDL ve Hata Düzeltme Modelleri yardımı ile çeşitli kontrol değişkenler üzerinden test edilmiştir. 1991-2019 yılı verileri kullanılarak yapılan analiz sonuçlarına göre turizm gelişiminin kısa dönemde tarımda kadın istihdamını negatif, uzun dönemdeyse hizmet sektöründe kadın istihdamını pozitif etkilediği belirlenmiştir. Ayrıca kadınların girişimcilik faaliyetlerinin tarım ve hizmet sektöründe istihdam açısından pozitif etkiler gösterdiği ve lise eğitiminin uzun dönemde her iki sektörde de etki göstermediği sonuçlarına ulaşılmıştır. Turizm sektörünün gelişimine dair uygulanacak politikaların aynı zamanda kadınların güçlendirilmesi için gerekli olan istihdam artışını da destekleyeceği öngörülmektedir. Bununla birlikte kadınların girişimciliğini destekleyen, cinsiyet eşitlikçi ve mesleki eğitimleri arttırıcı uygulamaların da mezkur politikaların içerisinde yer alması önem arz etmektedir.
Antioxidant and antimicrobial activities of methanol extracts from Adonis paryadrica (Ranunculaceae) – a critically endangered endemic species growing in the Turkish flora
Authors: CÜCE Mustafa
Published: March 2023

This study was conducted to determine the antioxidant and antimicrobial activities of methanol extract obtained from flower, leaf and root sections of endemic Adonis paryadrica (Boiss.) Kandemir & Aytaç stat. nova. naturally growing in the Turkish flora. The most efficient total phenolic compounds and flavonoid contents were obtained from leaf extract at 21.24 mg GAEs (gallic acid equivalent)/g dw and 54.97 mg REs (rutin equivalent)/g dw, respectively. Among the three different sections of this plant, leaf extracts showed the highest Cupric Reducing Antioxidant Power (CUPRAC) effect with 80.28 µmol TEs (trolox equivalent)/g dw. From the three different sections, the methanol extract of the leaf parts demonstrated strong antibacterial activity against Bacillus subtilis with a 16.1 mm zone diameter. These valuable and current findings from these precious plants, which constitute natural resources in terms of biodiversity, contribute innovative information to the literature on endemic plant species.