bluuebunny/crossref_metadata_2025 huggingface dataset:
An Experimental Validation of Numerical Model for Top-Hat Tubular Structure Subjected to Axial Crush
Authors: Abdulqadir Samer Fakhri , Tarlochan Faris
Published: May 2021

Vehicle crashworthiness is an important aspect to consider when designing a vehicle to ensure the safety of the occupants. Besides this, vehicles are also designed to reduce weight for better fuel economics. One possible approach to reducing weight without compromising vehicle safety is by looking at new designs and usage of composite materials, along with the usage of computational models to reduce time and cost. Hence, this paper displays the experimental results of a carbon fiber reinforced closed top-hat section subjected to both quasi-static and dynamic crushing loading. The results were used to validate the computational model developed in the study. The specimens were made of carbon composite prepregs MTM-44 sheets stacked at the alternative orientation of ±45° and 0°/90°, where 0° direction coincides with the axis of the member. The samples were prepared by using a mold and carbon prepregs under vacuum bagging followed by curing in an autoclave. Trigger initiation was applied to ensure the specimens demonstrated a stable crushing mode of failure during the test. Experimental investigations were carried out under the ambient conditions with different loading conditions, and different kinetic energy ranges (2, 3 and 6 kJ). Experimental data was used to validate the finite element analysis (FEA). The maximum errors obtained between experimental and FEA for mean load, mean energy absorption, and crushing displacement were 13%, 13% and 7%, respectively. The numerically obtained results were in strong agreement with the experimental data and showed that they were able to predict the failure of the specimens. The work also showed the novelty of using such structures for energy absorption applications.
An Empirical Study on Software Defect Prediction Using CodeBERT Model
Authors: Pan Cong , Lu Minyan , Xu Biao
Published: May 2021

Deep learning-based software defect prediction has been popular these days. Recently, the publishing of the CodeBERT model has made it possible to perform many software engineering tasks. We propose various CodeBERT models targeting software defect prediction, including CodeBERT-NT, CodeBERT-PS, CodeBERT-PK, and CodeBERT-PT. We perform empirical studies using such models in cross-version and cross-project software defect prediction to investigate if using a neural language model like CodeBERT could improve prediction performance. We also investigate the effects of different prediction patterns in software defect prediction using CodeBERT models. The empirical results are further discussed.
Changes in Abrasive Wear Resistance during Miller Test of High-Manganese Cast Steel with Niobium Carbides Formed in the Alloy Matrix
Authors: Tęcza Grzegorz
Published: May 2021

High-manganese Hadfield cast steel is commonly used for machine components operating under dynamic load conditions. The high fracture toughness and abrasive wear resistance of this steel are the result of an austenitic structure, which—while being ductile—at the same time tends to surface harden under the effect of cold work. Absence of dynamic loads (e.g., in the case of sand abrasion) causes rapid and premature wear of parts. To improve the abrasive wear resistance of high-manganese cast steel for operation under the conditions free from dynamic loads, primary niobium carbides are produced in this cast steel during the melting process to obtain in castings, after melt solidification, the microstructure consisting of an austenitic matrix and primary niobium carbides uniformly distributed in this matrix. The measured hardness of the tested samples as cast and after solution heat treatment is 260–290 HV and is about 30–60 HV higher than the hardness of common Hadfield cast steel, which is 230 HV. Compared to common Hadfield cast steel, the abrasive wear resistance of the tested high-manganese cast steel measured in the Miller test is at least three times higher at the niobium content of 3.5 wt%. Increasing the niobium content to 4.5 wt%. in the tested samples increases this wear resistance even more.
Software Tools for Conducting Real-Time Information Processing and Visualization in Industry: An Up-to-Date Review
Authors: Sousa Regina , Miranda Rui , Moreira Ailton , Alves Carlos , Lori Nicolas , Machado José
Published: May 2021

The processing of information in real-time (through the processing of complex events) has become an essential task for the optimal functioning of manufacturing plants. Only in this way can artificial intelligence, data extraction, and even business intelligence techniques be applied, and the data produced daily be used in a beneficent way, enhancing automation processes and improving service delivery. Therefore, professionals and researchers need a wide range of tools to extract, transform, and load data in real-time efficiently. Additionally, the same tool supports or at least facilitates the visualization of this data intuitively and interactively. The review presented in this document aims to provide an up-to-date review of the various tools available to perform these tasks. Of the selected tools, a brief description of how they work, as well as the advantages and disadvantages of their use, will be presented. Furthermore, a critical analysis of overall operation and performance will be presented. Finally, a hybrid architecture that aims to synergize all tools and technologies is presented and discussed.
The Ability to Control VOC Emissions from Multilayer Building Materials
Authors: Piasecki Michał , Kostyrko Krystyna Barbara , Goljan Anna
Published: May 2021

The work aimed to investigate which parameters of the electrically powered radiant floor heating system are connected with the intensity of VOC total emissions and emissions from individual layers, which can be effectively changed and controlled to obtain energy savings in the ventilation process. For this purpose, experimental studies of VOC emissions from specially designed LRFHS samples (Laboratory Radiant Floor Heating System) were carried out, along with simulations of real thermal conditions of samples of layered systems containing separate heaters and various materials layers. The TD-GC-MS chromatography was used to assess the trends of VOCs concentration changes in 480 h in a test chamber (simulating real conditions) for several LRFHS systems of multilayer construction products with built-in individual heating systems, in two stabilised temperatures, 23 °C and 33 °C, two stabilised relative humidities, 50% and 80% and three air exchanges per hour ACH on levels 0.5, 1.0 and 1.5. The obtained results indicate that the models used to determine emissions from single-layer products correspond to the description of emissions from multilayer systems only to a limited extent; some inner layers of floor systems are giving diffusion resistance or intensification of diffusion. A new emission model is proposed. The time-emission concentration curves for dry and wet environments differ significantly; reducing the VOC concentration in the air for the number of exchanges above 1.0 ACH is relatively inefficient. Authors also mapped out new research directions; for example, the experiment showed that not all of the VOC contaminants are ventilated just as easily and perhaps, considering their concentration of resistant impurities, chemical structure and diffusion resistance through the layers, there is a need to determine their weights.
Roman Fortress Pitiunt: 3D-Reconstruction of the Monument Based on the Materials of Archaeological Research and Geological Paleoreconstructions
Authors: Trebeleva Galina , Glazov Konstantin , Kizilov Andrey , Sakania Suram , Yurkov Vladlen , Yurkov Gleb
Published: May 2021

The present study examined the references in the works of ancient authors to the ancient city and the Roman fortress Pitiunt, the geological aspects of the formation of the coastline in the Pitsunda Cape area in the first centuries AD and the results of archaeological research of the monument performed from 1952 to 1974. The creation of the 3D reconstruction of the exterior of the Pitiunt fortress during its prosperity in the IV century AD, along with the churches which were the first monuments of religious architecture in northwestern Colchis (northwestern Colchis comprises parts of the territory of modern Russia, Georgia and Abkhazia) was carried out based on the excavation plans and the principles of fortification and temple architecture that were accepted in the late Roman times, paying special attention to the geological paleoreconstructions.
Filtering-Based Regularized Sparsity Variable Step-Size Matching Pursuit and Its Applications in Vehicle Health Monitoring
Authors: Liu Haoqiang , Zhao Hongbo , Feng Wenquan
Published: May 2021

Recent years have witnessed that real-time health monitoring for vehicles is gaining importance. Conventional monitoring scheme faces formidable challenges imposed by the massive signals generated with extremely heavy burden on storage and transmission. To address issues of signal sampling and transmission, compressed sensing (CS) has served as a promising solution in vehicle health monitoring, which performs signal sampling and compression simultaneously. Signal reconstruction is regarded as the most critical part of CS, while greedy reconstruction has been a research hotspot. However, the existing approaches either require prior knowledge of the sparse signal or perform with expensive computational complexity. To exploit the structure of the sparse signal, in this paper, we introduce an initial estimation approach for signal sparsity level firstly. Then, a novel greedy reconstruction algorithm that relies on no prior information of sparsity level while maintaining a good reconstruction performance is presented. The proposed algorithm integrates strategies of regularization and variable adaptive step size and further performs filtration. To verify the efficiency of the algorithm, typical voltage disturbance signals generated by the vehicle power system are taken as trial data. Preliminary simulation results demonstrate that the proposed algorithm achieves superior performance compared to the existing methods.
Awareness of Medication-Related Osteonecrosis of the Jaws amongst Dental Professionals in Poland
Authors: Teślak Monika , Sobczak Hanna , Ordyniec-Kwaśnica Iwona , Kochańska Barbara , Drogoszewska Barbara
Published: May 2021

Background: Medication-Related Osteonecrosis of the Jaws (MRONJ) is a serious complication of antiresorptive therapy. The aim of the study was to assess the level of knowledge and awareness regarding MRONJ among Polish dentists and students of dentistry. Methods: The online self-administered questionnaire was sent out electronically among dentists in Poland and dentistry students of the Medical University of Gdansk. The results obtained were statistically analyzed. The results were considered statistically significant if the condition p ≤ 0.05 was met. Results: A total of 203 respondents participated in this survey. A total of 94.6% of them declared knowledge of the term MRONJ. However, the length of bisphosphonates persistence in the body was known to 51.5% of participants, while the knowledge of oral and maxillofacial surgeons concerning this topic was significantly higher (87.0%). Conclusion: Dental and maxillofacial surgeons presented the highest level of knowledge about MRONJ. The remaining groups did not differ in their knowledge. It must be noted that the knowledge of the students was similar to that of dentists. A better level of knowledge by dentists could contribute to a decline of the occurrence of the disease in future. Theoretical and practical initiatives should be promoted to improve the knowledge about MRONJ.
Digital Twin: Origin to Future
Authors: Singh Maulshree , Fuenmayor Evert , Hinchy Eoin , Qiao Yuansong , Murray Niall , Devine Declan
Published: May 2021

Digital Twin (DT) refers to the virtual copy or model of any physical entity (physical twin) both of which are interconnected via exchange of data in real time. Conceptually, a DT mimics the state of its physical twin in real time and vice versa. Application of DT includes real-time monitoring, designing/planning, optimization, maintenance, remote access, etc. Its implementation is expected to grow exponentially in the coming decades. The advent of Industry 4.0 has brought complex industrial systems that are more autonomous, smart, and highly interconnected. These systems generate considerable amounts of data useful for several applications such as improving performance, predictive maintenance, training, etc. A sudden influx in the number of publications related to ‘Digital Twin’ has led to confusion between different terminologies related to the digitalization of industries. Another problem that has arisen due to the growing popularity of DT is a lack of consensus on the description of DT as well as so many different types of DT, which adds to the confusion. This paper intends to consolidate the different types of DT and different definitions of DT throughout the literature for easy identification of DT from the rest of the complimentary terms such as ‘product avatar’, ‘digital thread’, ‘digital model’, and ‘digital shadow’. The paper looks at the concept of DT since its inception to its predicted future to realize the value it can bring to certain sectors. Understanding the characteristics and types of DT while weighing its pros and cons is essential for any researcher, business, or sector before investing in the technology.
Ensemble Dispersion Simulation of a Point-Source Radioactive Aerosol Using Perturbed Meteorological Fields over Eastern Japan
Authors: Sekiyama Tsuyoshi Thomas , Kajino Mizuo , Kunii Masaru
Published: May 2021

We conducted single-model initial-perturbed ensemble simulations to quantify uncertainty in aerosol dispersion modeling, focusing on a point-source radioactive aerosol emitted from the Fukushima Daiichi Nuclear Power Plant (FDNPP) in March 2011. The ensembles of the meteorological variables were prepared using a data assimilation system that consisted of a non-hydrostatic weather-forecast model with a 3-km horizontal resolution and a four-dimensional local ensemble transform Kalman filter (4D-LETKF) with 20 ensemble members. The emission of radioactive aerosol was not perturbed. The weather and aerosol simulations were validated with in-situ measurements at Hitachi and Tokai, respectively, approximately 100 km south of the FDNPP. The ensemble simulations provided probabilistic information and multiple case scenarios for the radioactive aerosol plumes. Some of the ensemble members successfully reproduced the arrival time and intensity of the radioactive aerosol plumes, even when the deterministic simulation failed to reproduce them. We found that a small ensemble spread of wind speed produced large uncertainties in aerosol concentrations.
Climatological Increased Precipitation from July to August in the Western North Pacific Region Simulated by CMIP6 Models
Authors: Dong Xiao , Lin Renping
Published: May 2021

In this study, the climatological precipitation increase from July to August over the western North Pacific (WNP) region was investigated through observations and simulations in the Coupled Model Intercomparison Project Phase 6 (CMIP6), atmospheric model simulations and historical experiments. Firstly, observational analysis showed that the precipitation increase is associated with a decrease in the local sea surface temperature (SST), indicating that the precipitation increase is not driven by the change in SST. In addition, the pattern of precipitation increase is similar to the vertical motion change at 500-hPa, suggesting that the precipitation increase is related to the circulation change. Moisture budget analysis further confirmed this relation. In addition to the observational analysis, the outputs from 26 CMIP6 models were further evaluated. Compared with atmospheric model simulations, air–sea coupled models largely improve the simulation of the climatological precipitation increase from July to August. Furthermore, model simulations confirmed that the bias in the precipitation increase is intimately associated with the circulation change bias. Thus, two factors are responsible for the bias of the precipitation increase from July to August in climate models: air–sea coupling processes and the performance in vertical motion change.
Common Fixed Point Results for Almost ℛg-Geraghty Type Contraction Mappings in b2-Metric Spaces with an Application to Integral Equations
Authors: Saleh Samera M. , Sessa Salvatore , Alfaqih Waleed M. , Shaddad Fawzia
Published: May 2021

In this paper, we define almost Rg-Geraghty type contractions and utilize the same to establish some coincidence and common fixed point results in the setting of b2-metric spaces endowed with binary relations. As consequences of our newly proved results, we deduce some coincidence and common fixed point results for almost g-α-η Geraghty type contraction mappings in b2-metric spaces. In addition, we derive some coincidence and common fixed point results in partially ordered b2-metric spaces. Moreover, to show the utility of our main results, we provide an example and an application to non-linear integral equations.
Metatranscriptomic Analysis Reveals an Imbalance of Hepatopancreatic Flora of Chinese Mitten Crab Eriocheir sinensis with Hepatopancreatic Necrosis Disease
Authors: Shen Zeen , Kumar Dhiraj , Liu Xunmeng , Yan Bingyu , Fang Ping , Gu Yuchao , Li Manyun , Xie Meiping , Yuan Rui , Feng Yongjie , Hu Xiaolong , Cao Guangli , Xue Renyu , Chen Hui , Liu Xiaohan , Gong Chengliang
Published: May 2021

Hepatopancreas necrosis disease (HPND) of the Chinese mitten crab Eriocheir sinensis causes huge economic loss in China. However, the pathogenic factors and pathogenesis are still a matter of dissension. To search for potential pathogens, the hepatopancreatic flora of diseased crabs with mild symptoms, diseased crabs with severe symptoms, and crabs without visible symptoms were investigated using metatranscriptomics sequencing. The prevalence of Absidia glauca and Candidatus Synechococcus spongiarum decreased, whereas the prevalence of Spiroplasma eriocheiris increased in the hepatopancreatic flora of crabs with HPND. Homologous sequences of 34 viral species and 4 Microsporidian species were found in the crab hepatopancreas without any significant differences between crabs with and without HPND. Moreover, DEGs in the hepatopancreatic flora between crabs with severe symptoms and without visible symptoms were enriched in the ribosome, retinol metabolism, metabolism of xenobiotics by cytochrome P450, drug metabolism—cytochrome P450, biosynthesis of unsaturated fatty acids, and other glycan degradation. Moreover, the relative abundance of functions of DEDs in the hepatopancreatic flora changed with the pathogenesis process. These results suggested that imbalance of hepatopancreatic flora was associated with crab HPND. The identified DEGs were perhaps involved in the pathological mechanism of HPND; nonetheless, HPND did not occur due to virus or microsporidia infection.
Oximes: Novel Therapeutics with Anticancer and Anti-Inflammatory Potential
Authors: Schepetkin Igor A. , Plotnikov Mark B. , Khlebnikov Andrei I. , Plotnikova Tatiana M. , Quinn Mark T.
Published: May 2021

Oximes have been studied for decades because of their significant roles as acetylcholinesterase reactivators. Over the last twenty years, a large number of oximes have been reported with useful pharmaceutical properties, including compounds with antibacterial, anticancer, anti-arthritis, and anti-stroke activities. Many oximes are kinase inhibitors and have been shown to inhibit over 40 different kinases, including AMP-activated protein kinase (AMPK), phosphatidylinositol 3-kinase (PI3K), cyclin-dependent kinase (CDK), serine/threonine kinases glycogen synthase kinase 3 α/β (GSK-3α/β), Aurora A, B-Raf, Chk1, death-associated protein-kinase-related 2 (DRAK2), phosphorylase kinase (PhK), serum and glucocorticoid-regulated kinase (SGK), Janus tyrosine kinase (JAK), and multiple receptor and non-receptor tyrosine kinases. Some oximes are inhibitors of lipoxygenase 5, human neutrophil elastase, and proteinase 3. The oxime group contains two H-bond acceptors (nitrogen and oxygen atoms) and one H-bond donor (OH group), versus only one H-bond acceptor present in carbonyl groups. This feature, together with the high polarity of oxime groups, may lead to a significantly different mode of interaction with receptor binding sites compared to corresponding carbonyl compounds, despite small changes in the total size and shape of the compound. In addition, oximes can generate nitric oxide. This review is focused on oximes as kinase inhibitors with anticancer and anti-inflammatory activities. Oximes with non-kinase targets or mechanisms of anti-inflammatory activity are also discussed.
DEER Analysis of GPCR Conformational Heterogeneity
Authors: Elgeti Matthias , Hubbell Wayne L.
Published: May 2021

G protein-coupled receptors (GPCRs) represent a large class of transmembrane helical proteins which are involved in numerous physiological signaling pathways and therefore represent crucial pharmacological targets. GPCR function and the action of therapeutic molecules are defined by only a few parameters, including receptor basal activity, ligand affinity, intrinsic efficacy and signal bias. These parameters are encoded in characteristic receptor conformations existing in equilibrium and their populations, which are thus of paramount interest for the understanding of receptor (mal-)functions and rational design of improved therapeutics. To this end, the combination of site-directed spin labeling and EPR spectroscopy, in particular double electron–electron resonance (DEER), is exceedingly valuable as it has access to sub-Angstrom spatial resolution and provides a detailed picture of the number and populations of conformations in equilibrium. This review gives an overview of existing DEER studies on GPCRs with a focus on the delineation of structure/function frameworks, highlighting recent developments in data analysis and visualization. We introduce “conformational efficacy” as a parameter to describe ligand-specific shifts in the conformational equilibrium, taking into account the loose coupling between receptor segments observed for different GPCRs using DEER.
Epithelial to Mesenchymal Transition History: From Embryonic Development to Cancers
Authors: Lachat Camille , Peixoto Paul , Hervouet Eric
Published: May 2021

Epithelial to mesenchymal transition (EMT) is a process that allows epithelial cells to progressively acquire a reversible mesenchymal phenotype. Here, we recount the main events in the history of EMT. EMT was first studied during embryonic development. Nowadays, it is an important field in cancer research, studied all around the world by more and more scientists, because it was shown that EMT is involved in cancer aggressiveness in many different ways. The main features of EMT’s involvement in embryonic development, fibrosis and cancers are briefly reviewed here.
Harnessing the Physiological Functions of Cellular Prion Protein in the Kidneys: Applications for Treating Renal Diseases
Authors: Yoon Sungtae , Go Gyeongyun , Yoon Yeomin , Lim Jiho , Lee Gaeun , Lee Sanghun
Published: May 2021

A cellular prion protein (PrPC) is a ubiquitous cell surface glycoprotein, and its physiological functions have been receiving increased attention. Endogenous PrPC is present in various kidney tissues and undergoes glomerular filtration. In prion diseases, abnormal prion proteins are found to accumulate in renal tissues and filtered into urine. Urinary prion protein could serve as a diagnostic biomarker. PrPC plays a role in cellular signaling pathways, reno-protective effects, and kidney iron uptake. PrPC signaling affects mitochondrial function via the ERK pathway and is affected by the regulatory influence of microRNAs, small molecules, and signaling proteins. Targeting PrPC in acute and chronic kidney disease could help improve iron homeostasis, ameliorate damage from ischemia/reperfusion injury, and enhance the efficacy of mesenchymal stem/stromal cell or extracellular vesicle-based therapeutic strategies. PrPC may also be under the influence of BMP/Smad signaling and affect the progression of TGF-β-related renal fibrosis. PrPC conveys TNF-α resistance in some renal cancers, and therefore, the coadministration of anti-PrPC antibodies improves chemotherapy. PrPC can be used to design antibody–drug conjugates, aptamer–drug conjugates, and customized tissue inhibitors of metalloproteinases to suppress cancer. With preclinical studies demonstrating promising results, further research on PrPC in the kidney may lead to innovative PrPC-based therapeutic strategies for renal disease.
Epipregnanolone as a Positive Modulator of GABAA Receptor in Rat Cerebellar and Hippocampus Neurons
Authors: Bukanova Julia , Solntseva Elena , Kondratenko Rodion , Kudova Eva
Published: May 2021

Epipregnanolone (3β-hydroxy-5β-pregnan-20-one, Epi) is an endogenous steroid with important physiological effects and high affinity for GABAA receptors. The effect of Epi on GABA-induced chloride current (IGABA) in native neurons has hardly been studied. In this work, we studied the influence of Epi on the IGABA in the Purkinje cells of rat cerebellum and pyramidal neurons of rat hippocampus with the patch clamp technique. We showed that Epi is a positive modulator of the IGABA with EC50 of 5.7 µM in Purkinje cells and 9.3 µM in hippocampal neurons. Epi-induced potentiation of the IGABA was more potent at low vs. high GABA concentrations. Isopregnanolone (3β-hydroxy-5α-pregnan-20-one, Iso) counteracted Epi, reducing its potentiating effect by 2–2.3 times. Flumazenil, a nonsteroidal GABAA receptor antagonist, does not affect the Epi-induced potentiation. Comparison of the potentiating effects of Epi and allopregnanolone (3α-hydroxy-5α-pregnan-20-one, ALLO) showed that ALLO is, at least, a four times more potent positive modulator than Epi. The combined application of ALLO and Epi showed that the effects of these two steroids are not additive. We conclude that Epi has a dual effect on the IGABA increasing the current in the control solution and decreasing the stimulatory effect of ALLO.
Influence of the luxR Regulatory Gene Dosage and Expression Level on the Sensitivity of the Whole-Cell Biosensor to Acyl-Homoserine Lactone
Authors: Bazhenov Sergey , Novoyatlova Uliana , Scheglova Ekaterina , Fomin Vadim , Khrulnova Svetlana , Melkina Olga , Chistyakov Vladimir , Manukhov Ilya
Published: May 2021

Aliivibrio fischeri LuxR and Aliivibrio logei LuxR1 and LuxR2 regulatory proteins are quorum sensing transcriptional (QS) activators, inducing promoters of luxICDABEG genes in the presence of an autoinducer (3-oxo-hexanoyl-l-homoserine lactone). In the Aliivibrio cells, luxR genes are regulated by HNS, CRP, LitR, etc. Here we investigated the role of the luxR expression level in LuxI/R QS system functionality and improved the whole-cell biosensor for autoinducer detection. Escherichia coli-based bacterial lux-biosensors were used, in which Photorhabdus luminescensluxCDABE genes were controlled by LuxR-dependent promoters and luxR, luxR1, or luxR2 regulatory genes. We varied either the dosage of the regulatory gene in the cells using additional plasmids, or the level of the regulatory gene expression using the lactose operon promoter. It was shown that an increase in expression level, as well as dosage of the regulatory gene in biosensor cells, leads to an increase in sensitivity (the threshold concentration of AI is reduced by one order of magnitude) and to a two to threefold reduction in response time. The best parameters were obtained for a biosensor with an increased dosage of luxRA. fischeri (sensitivity to 3-oxo-hexanoyl-l-homoserine lactone reached 30–100 pM).
Implementation of an Automated Cerebrospinal Fluid Drainage System for Early Mobilization in Neurosurgical Patients
Authors: Arts Sebastian , van Bilsen Martine , van Lindert Erik J. , Bartels Ronald HMA , Aquarius Rene , Boogaarts Hieronymus D.
Published: May 2021

Background: Automated cerebrospinal fluid (CSF) drainage systems allow for the mobilization of patients with an external CSF drain. The aim of this study is to describe the implementation of an automated CSF drainage system in neurosurgical patients with external CSF drains. Methods: A feasibility study was performed using an automated CSF drainage system (LiquoGuard®7, Möller Medical GmbH, Fulda, Germany) in adult neurosurgical patients treated with external lumbar or external ventricular drains between December 2017 and June 2020. Limited mobilization was allowed—patients were allowed to adjust their inclined beds, sit in chairs and walk under the supervision of a nurse or physical therapist. The primary outcome was the number of prematurely terminated drainage sessions. Results: Twenty-three patients were included. Drainage was terminated prematurely in eight (35%) patients. In three (13%) of these patients, drainage was terminated due to signs of hydrocephalus. Pressure-controlled drainage in patients with external lumbar drains (ELD) showed inaccurate pressure curves, which was solved by using volume-controlled drainage in ELD patients. Conclusion: The implementation of an automated CSF drainage system (LiquoGuard®7) for CSF drainage allows for early mobilization in a subset of patients with external CSF drains. External lumbar drains require volume-based drainage rather than differential pressure-dependent drainage.
The Impact of Exercise Intervention with Rhythmic Auditory Stimulation to Improve Gait and Mobility in Parkinson Disease: An Umbrella Review
Authors: Forte Roberta , Tocci Nicoletta , De Vito Giuseppe
Published: May 2021

Difficulties in walking, controlling balance, and performing activities of daily living are common problems encountered by individuals affected by Parkinson disease. Scientific evidence suggests that exercise performed with music or auditory or rhythmical cues facilitates movement and improves balance, gait, mobility, and activities of daily living (ADL) performance in patients with PD. The aim of this umbrella review was to summarize available high-quality evidence from systematic reviews and meta-analyses on the effectiveness of rhythmically cued exercise to improve gait, mobility, and ADL performance in individuals with PD. PubMed, Cochrane, and Embase databases were searched from January 2010 to October 2020 for systematic reviews and meta-analyses which had to be (1) written in English, (2) include studies on populations of males and females with PD of any age, (3) analyze outcomes related to gait, mobility, and ADL, and (4) apply exercise interventions with music or auditory or rhythmical cues. Two independent authors screened potentially eligible studies and assessed the methodological quality of the studies using the AMSTAR 2 tool. Four studies, two systematic reviews and meta-analyses, one a systematic review, and one a meta-analysis, were selected. Overall results indicated positive effects for gait and mobility of the use of rhythmic auditory cueing with exercise and suggested that it should be incorporated into a regular rehabilitation program for patients affected by PD. Nonetheless, more primary level research is needed to address the identified gaps regarding the application of this method to physical exercise interventions.
Reaction Time and Visual Memory in Connection with Alcohol Use in Schizophrenia and Schizoaffective Disorder
Authors: Mazumder Atiqul Haq , Barnett Jennifer , Lindberg Nina , Torniainen-Holm Minna , Lähteenvuo Markku , Lahdensuo Kaisla , Kerkelä Martta , Hietala Jarmo , Isometsä Erkki Tapio , Kampman Olli , Kieseppä Tuula , Jukuri Tuomas , Häkkinen Katja , Cederlöf Erik , Haaki Willehard , Kajanne Risto , Wegelius Asko , Männynsalo Teemu , Niemi-Pynttäri Jussi , Suokas Kimmo , Lönnqvist Jouko , Niemelä Solja , Tiihonen Jari , Paunio Tiina , Palotie Aarno , Suvisaari Jaana , Veijola Juha
Published: May 2021

The purpose of this study was to explore the association between cognition and hazardous drinking and alcohol use disorder in schizophrenia and schizoaffective disorder. Cognition is more or less compromised in schizophrenia, and schizoaffective disorder and alcohol use might aggravate this phenomenon. The study population included 3362 individuals from Finland with diagnoses of schizophrenia or schizoaffective disorder. Hazardous drinking was screened with the AUDIT-C (Alcohol Use Disorders Identification Test for Consumption) screening tool. Alcohol use disorder (AUD) diagnoses were obtained from national registrar data. Participants performed two computerized tasks from the Cambridge Automated Neuropsychological Test Battery (CANTAB) on a tablet computer: The Five-Choice Serial Reaction Time Task (5-CSRTT) or the reaction time (RT) test and the Paired Associative Learning (PAL) test. The association between alcohol use and the RT and PAL tests was analyzed with log-linear regression and logistic regression, respectively. After adjustment for age, education, housing status, and the age at which the respondents had their first psychotic episodes, hazardous drinking was associated with a lower median RT in females and less variable RT in males, while AUD was associated with a poorer PAL test performance in terms of the total errors adjusted scores (TEASs) in females. Our findings of positive associations between alcohol and cognition in schizophrenia and schizoaffective disorder are unique.
Reactive Deep Eutectic Solvents (RDESs): A New Tool for Phospholipase D-Catalyzed Preparation of Phospholipids
Authors: Allegretti Chiara , Gatti Francesco G. , Marzorati Stefano , Rossato Letizia Anna Maria , Serra Stefano , Strini Alberto , D’Arrigo Paola
Published: May 2021

The use of Reactive Deep Eutectic Solvents (RDESs) in the preparation of polar head modified phospholipids (PLs) with phospholipase D (PLD)-catalyzed biotransformations has been investigated. Natural phosphatidylcholine (PC) has been submitted to PLD-catalyzed transphosphatidylations using a new reaction medium composed by a mixture of RDES/buffer. Instead of exploiting deep eutectic solvents conventionally, just as the reaction media, these solvents have been designed here in order to contribute actively to the synthetic processes by participating as reagents. RDESs were prepared using choline chloride or trimethyl glycine as hydrogen-bond acceptors and glycerol or ethylene glycol, as hydrogen-bond donors as well as nucleophiles for choline substitution. Specifically designed RDES/buffer reaction media allowed the obtainment of PLs with optimized yields in the perspective of a sustainable process implementation.
Bio-DEE Synthesis and Dehydrogenation Coupling of Bio-Ethanol to Bio-Butanol over Multicomponent Mixed Metal Oxide Catalysts
Authors: Pieta Izabela S. , Michalik Alicja , Kraleva Elka , Mrdenovic Dusan , Sek Alicja , Wahaczyk Ewa , Lewalska-Graczyk Agnieszka , Krysa Mikolaj , Sroka-Bartnicka Anna , Pieta Piotr , Nowakowski Robert , Lew Agata , Serwicka Ewa M.
Published: May 2021

Within the Waste2Fuel project, innovative, high-performance, and cost-effective fuel production methods from municipal solid wastes (MSWs) are sought for application as energy carriers or direct drop-in fuels/chemicals in the near-future low-carbon power generation systems and internal combustion engines. Among the studied energy vectors, C1-C2 alcohols and ethers are mainly addressed. This study presents a potential bio-derived ethanol oxidative coupling in the gas phase in multicomponent systems derived from hydrotalcite-containing precursors. The reaction of alcohol coupling to ethers has great importance due to their uses in different fields. The samples have been synthesized by the co-precipitation method via layered double hydroxide (LDH) material synthesis, with a controlled pH, where the M(II)/M(III) ≈ 0.35. The chemical composition and topology of the sample surface play essential roles in catalyst activity and product distribution. The multiple redox couples Ni2+/Ni3+, Cr2+/Cr3+, Mn2+/Mn3+, and the oxygen-vacant sites were considered as the main active sites. The introduction of Cr (Cr3+/Cr4+) and Mn (Mn3+/Mn4+) into the crystal lattice could enhance the number of oxygen vacancies and affect the acid/base properties of derived mixed oxides, which are considered as crucial parameters for process selectivity towards bio-DEE and bio-butanol, preventing long CH chain formation and coke deposition at the same time.
Efficient Advanced Oxidation Process (AOP) for Photocatalytic Contaminant Degradation Using Exfoliated Metal-Free Graphitic Carbon Nitride and Visible Light-Emitting Diodes
Authors: Rana Adeem Ghaffar , Tasbihi Minoo , Schwarze Michael , Minceva Mirjana
Published: May 2021

The photocatalytic performance of metal-free graphitic carbon nitride (g-C3N4) was examined using visible light-emitting diodes (LEDs). A comparative and parametric study was conducted using the photocatalytic degradation of phenol as a model reaction. The g-C3N4 photocatalyst was synthesized from melamine using thermal condensation, followed by a thermal exfoliation that increases the catalyst surface area from 11 to 170 m2/g. Different characterization techniques, namely X-ray powder diffraction, X-ray photoelectron spectroscopy, nitrogen adsorption using the Brunauer–Emmett–Teller method, ultraviolet-visible (UV–vis) spectroscopy, transmission electron microscopy, photoluminescence spectroscopy (PL), and zeta potential analysis, were used to characterize the photocatalyst. A comparison of the photodegradation experiments conducted with a full-spectrum xenon lamp and a custom-made single-wavelength LED immersion lamp showed that the photocatalyst performance was better with the LED immersion lamp. Furthermore, a comparison of the performance of exfoliated and bulk g-C3N4 revealed that exfoliated g-C3N4 completely degraded the pollutant in 90 min, whereas only 25% was degraded with bulk g-C3N4 in 180 min because the exfoliated g-C3N4 enhances the availability of active sites, which promotes the degradation of phenol. Experiments conducted at different pH have shown that acidic pH favors the degradation process. The exfoliated g-C3N4 has shown high photocatalytic performance in the photodegradation of other phenolic compounds, such as catechol, m-cresol, and xylenol, as well.
Materials and Processes for Photocatalytic and (Photo)Electrocatalytic Removal of Bio-Refractory Pollutants and Emerging Contaminants from Waters
Authors: Vacca Annalisa
Published: May 2021

This volume is focused on materials and processes for the electro- and photoelectrochemical removal of biorefractory pollutants and emerging contaminants from waters to show the importance of electrochemistry and photoelectrochemistry in offering solutions to current environmental problems [...]
Design of Iridium N-Heterocyclic Carbene Amino Acid Catalysts for Asymmetric Transfer Hydrogenation of Aryl Ketones
Authors: Bernier Chad M. , Merola Joseph S.
Published: May 2021

A series of chiral complexes of the form Ir(NHC)2(aa)(H)(X) (NHC = N-heterocyclic carbene, aa = chelated amino acid, X = halide) was synthesized by oxidative addition of α-amino acids to iridium(I) bis-NHC compounds and screened for asymmetric transfer hydrogenation of ketones. Following optimization of the reaction conditions, NHC, and amino acid ligands, high enantioselectivity was achieved when employing the Ir(IMe)2(l-Pro)(H)(I) catalyst (IMe = 1,3-dimethylimidazol-2-ylidene), which asymmetrically reduces a range of acetophenone derivatives in up to 95% enantiomeric excess.
The Effect of Atomic Layer Deposited Overcoat on Co-Pt-Si/γ-Al2O3 Fischer–Tropsch Catalyst
Authors: Heikkinen Niko , Keskiväli Laura , Eskelinen Patrik , Reinikainen Matti , Putkonen Matti
Published: May 2021

Atomic layer deposition (ALD) was used to prepare a thin alumina layer on Fischer–Tropsch catalysts. Co-Pt-Si/γ-Al2O3 catalyst was overcoated with 15–40 cycles of Al2O3 deposited from trimethylaluminum (TMA) and water vapor, followed by thermal annealing. The resulting tailored Fischer–Tropsch catalyst with 35 cycle ALD overcoating had increased activity compared to unmodified catalyst. The increase in activity was achieved without significant loss of selectivity towards heavier hydrocarbons. Altered catalyst properties were assumed to result from cobalt particle stabilization by ALD alumina overcoating and nanoscale porosity of the overcoating. In addition to optimal thickness of the overcoat, thermal annealing was an essential part of preparing ALD overcoated catalyst.
Rap1a Regulates Cardiac Fibroblast Contraction of 3D Diabetic Collagen Matrices by Increased Activation of the AGE/RAGE Cascade
Authors: Burr Stephanie D. , Stewart James A.
Published: May 2021

Cardiovascular disease is a common diabetic complication that can arise when cardiac fibroblasts transition into myofibroblasts. Myofibroblast transition can be induced by advanced glycated end products (AGEs) present in the extracellular matrix (ECM) activating RAGE (receptor for advanced glycated end products) to elicit intracellular signaling. The levels of AGEs are higher under diabetic conditions due to the hyperglycemic conditions present in diabetics. AGE/RAGE signaling has been shown to alter protein expression and ROS production in cardiac fibroblasts, resulting in changes in cellular function, such as migration and contraction. Recently, a small GTPase, Rap1a, has been identified to overlap the AGE/RAGE signaling cascade and mediate changes in protein expression. While Rap1a has been shown to impact AGE/RAGE-induced protein expression, there are currently no data examining the impact Rap1a has on AGE/RAGE-induced cardiac fibroblast function. Therefore, we aimed to determine the impact of Rap1a on AGE/RAGE-mediated cardiac fibroblast contraction, as well as the influence isolated diabetic ECM has on facilitating these effects. In order to address this idea, genetically different cardiac fibroblasts were embedded in 3D collagen matrices consisting of collagen isolated from either non-diabetic of diabetic mice. Fibroblasts were treated with EPAC and/or exogenous AGEs, which was followed by assessment of matrix contraction, protein expression (α-SMA, SOD-1, and SOD-2), and hydrogen peroxide production. The results showed Rap1a overlaps the AGE/RAGE cascade to increase the myofibroblast population and generation of ROS production. The increase in myofibroblasts and oxidative stress appeared to contribute to increased matrix contraction, which was further exacerbated by diabetic conditions. Based off these results, we determined that Rap1a was essential in mediating the response of cardiac fibroblasts to AGEs within diabetic collagen.
Extracellular Vesicles from Mesenchymal Stem Cells as Potential Treatments for Osteoarthritis
Authors: Mohd Noor Nur Azira , Abdullah Nurul Asma , Ahmad Mohd Zain Muhammad Rajaei , Wan Nor Aduni Wan Khairunnisaa , Azlan Maryam
Published: May 2021

Osteoarthritis (OA) is a chronic degenerative disorder of the joint and its prevalence and severity is increasing owing to ageing of the population. Osteoarthritis is characterized by the degradation of articular cartilage and remodeling of the underlying bone. There is little understanding of the cellular and molecular processes involved in pathophysiology of OA. Currently the treatment for OA is limited to painkillers and anti-inflammatory drugs, which only treat the symptoms. Some patients may also undergo surgical procedures to replace the damaged joints. Extracellular vesicles (EV) play an important role in intercellular communications and their concentration is elevated in the joints of OA patients, although their mechanism is unclear. Extracellular vesicles are naturally released by cells and they carry their origin cell information to be delivered to target cells. On the other hand, mesenchymal stem cells (MSCs) are highly proliferative and have a great potential in cartilage regeneration. In this review, we provide an overview of the current OA treatments and their limitations. We also discuss the role of EV in OA pathophysiology. Finally, we highlight the therapeutic potential of MSC-derived EV in OA and their challenges.
Generators of Pressure-Evoked Currents in Vertebrate Outer Retinal Neurons
Authors: Pang Ji-Jie , Gao Fan , Wu Samuel M.
Published: May 2021

(1) Background: High-tension glaucoma damages the peripheral vision dominated by rods. How mechanosensitive channels (MSCs) in the outer retina mediate pressure responses is unclear. (2) Methods: Immunocytochemistry, patch clamp, and channel fluorescence were used to study MSCs in salamander photoreceptors. (3) Results: Immunoreactivity of transient receptor potential channel vanilloid 4 (TRPV4) was revealed in the outer plexiform layer, K+ channel TRAAK in the photoreceptor outer segment (OS), and TRPV2 in some rod OS disks. Pressure on the rod inner segment evoked sustained currents of three components: (A) the inward current at <−50 mV (Ipi), sensitive to Co2+; (B) leak outward current at ≥−80 mV (Ipo), sensitive to intracellular Cs+ and ruthenium red; and (C) cation current reversed at ~10 mV (Ipc). Hypotonicity induced slow currents like Ipc. Environmental pressure and light increased the FM 1-43-identified open MSCs in the OS membrane, while pressure on the OS with internal Cs+ closed a Ca2+-dependent current reversed at ~0 mV. Rod photocurrents were thermosensitive and affected by MSC blockers. (4) Conclusions: Rods possess depolarizing (TRPV) and hyperpolarizing (K+) MSCs, which mediate mutually compensating currents between −50 mV and 10 mV, serve as an electrical cushion to minimize the impact of ocular mechanical stress.
Characterization of PARP6 Function in Knockout Mice and Patients with Developmental Delay
Authors: Vermehren-Schmaedick Anke , Huang Jeffrey Y. , Levinson Madison , Pomaville Matthew B. , Reed Sarah , Bellus Gary A. , Gilbert Fred , Keren Boris , Heron Delphine , Haye Damien , Janello Christine , Makowski Christine , Danhauser Katharina , Fedorov Lev M. , Haack Tobias B. , Wright Kevin M. , Cohen Michael S.
Published: May 2021

PARP6, a member of a family of enzymes (17 in humans) known as poly-ADP-ribose polymerases (PARPs), is a neuronally enriched PARP. While previous studies from our group show that Parp6 is a regulator of dendrite morphogenesis in rat hippocampal neurons, its function in the nervous system in vivo is poorly understood. Here, we describe the generation of a Parp6 loss-of-function mouse model for examining the function of Parp6 during neurodevelopment in vivo. Using CRISPR-Cas9 mutagenesis, we generated a mouse line that expressed a Parp6 truncated variant (Parp6TR) in place of Parp6WT. Unlike Parp6WT, Parp6TR is devoid of catalytic activity. Homozygous Parp6TR do not exhibit obvious neuromorphological defects during development, but nevertheless die perinatally. This suggests that Parp6 catalytic activity is important for postnatal survival. We also report PARP6 mutations in six patients with several neurodevelopmental disorders, including microencephaly, intellectual disabilities, and epilepsy. The most severe mutation in PARP6 (C563R) results in the loss of catalytic activity. Expression of Parp6C563R in hippocampal neurons decreases dendrite morphogenesis. To gain further insight into PARP6 function in neurons we also performed a BioID proximity labeling experiment in hippocampal neurons and identified several microtubule-binding proteins (e.g., MAP-2) using proteomics. Taken together, our results suggest that PARP6 is an essential microtubule-regulatory gene in mice, and that the loss of PARP6 catalytic activity has detrimental effects on neuronal function in humans.