bluuebunny/crossref_metadata_2025 huggingface dataset:
The Ameliorating Effects of n-3 Polyunsaturated Fatty Acids on Liver Steatosis Induced by a High-Fat Methionine Choline-Deficient Diet in Mice
Authors: Šmíd Václav , Dvořák Karel , Stehnová Kamila , Strnad Hynek , Rubert Josep , Stříteský Jan , Staňková Barbora , Stránská Milena , Hajšlová Jana , Brůha Radan , Vítek Libor
Published: December 2023

The pathogenesis of non-alcoholic fatty liver disease (NAFLD) is associated with abnormalities of liver lipid metabolism. On the contrary, a diet enriched with n-3 polyunsaturated fatty acids (n-3-PUFAs) has been reported to ameliorate the progression of NAFLD. The aim of our study was to investigate the impact of dietary n-3-PUFA enrichment on the development of NAFLD and liver lipidome. Mice were fed for 6 weeks either a high-fat methionine choline-deficient diet (MCD) or standard chow with or without n-3-PUFAs. Liver histology, serum biochemistry, detailed plasma and liver lipidomic analyses, and genome-wide transcriptome analysis were performed. Mice fed an MCD developed histopathological changes characteristic of NAFLD, and these changes were ameliorated with n-3-PUFAs. Simultaneously, n-3-PUFAs decreased serum triacylglycerol and cholesterol concentrations as well as ALT and AST activities. N-3-PUFAs decreased serum concentrations of saturated and monounsaturated free fatty acids (FAs), while increasing serum concentrations of long-chain PUFAs. Furthermore, in the liver, the MCD significantly increased the hepatic triacylglycerol content, while the administration of n-3-PUFAs eliminated this effect. Administration of n-3-PUFAs led to significant beneficial differences in gene expression within biosynthetic pathways of cholesterol, FAs, and pro-inflammatory cytokines (IL-1 and TNF-α). To conclude, n-3-PUFA supplementation appears to represent a promising nutraceutical approach for the restoration of abnormalities in liver lipid metabolism and the prevention and treatment of NAFLD.
The Involvement of Semaphorins in the Pathogenesis of Skin Diseases
Authors: Słuczanowska-Głąbowska Sylwia , Jankowska Olga , Staniszewska Marzena , Pawlik Andrzej
Published: December 2023

Semaphorins belong to a group of membrane and secretory proteins that act as ligands for several receptor families and are involved in modulating cell signaling pathways. They bind multimeric receptor complexes on the cell membrane to exert their effects and initiate unique intracellular signal transduction cascades. These proteins can influence several processes that are very important for cell function, such as cell division and differentiation. Semaphorins are involved in cell migration, apoptosis, cell adhesion, aggregation, and numerous immune processes due to their immunoregulatory effects. Semaphorins are expressed in keratinocytes, which is why they have become a target for studies on the pathogenesis of skin diseases. Most studies to date on the role of semaphorins in the pathogenesis of skin diseases have been carried out in cellular or animal models, and there are few clinical studies evaluating the role of semaphorins in the pathogenesis and therapy of skin diseases. In this narrative review, we summarized the current state of knowledge on the role of semaphorins in the pathogenesis of skin diseases and their potential importance as targets for therapy. We also tried to present the key findings and weaknesses of previous research in this field. The novelty of this article lies in the comprehensive presentation of the role of semaphorins in the pathogenesis of skin diseases, including the results of studies on cell cultures and animal models, elucidating the mechanisms and signaling pathways through which semaphorins affect the development of skin diseases, as well as on the presentation of the results of existing clinical trials evaluating the role of semaphorins in the pathogenesis of skin diseases, and as potential therapeutic targets.
Histidine-Bound Dinitrosyl Iron Complexes: Antioxidant and Antiradical Properties
Authors: Shumaev Konstantin B. , Kosmachevskaya Olga V. , Nasybullina Elvira I. , Ruuge Enno K. , Kalenikova Elena I. , Topunov Alexey F.
Published: December 2023

Dinitrosyl iron complexes (DNICs) are important physiological derivatives of nitric oxide. These complexes have a wide range of biological activities, with antioxidant and antiradical ones being of particular interest and importance. We studied the interaction between DNICs associated with the dipeptide L-carnosine or serum albumin and prooxidants under conditions mimicking oxidative stress. The ligands of these DNICs were histidine residues of carnosine or His39 and Cys34 in bovine serum albumin. Carnosine-bound DNICs reduced the level of piperazine free radicals in the reaction system containing tert-butyl hydroperoxide (t-BOOH), bivalent iron ions, a nitroxyl anion donor (Angeli’s salt), and HEPES buffer. The ability of carnosine DNICs to intercept organic free radicals produced from t-BOOH decay could lead to this effect. In addition, carnosine DNICs reacted with the superoxide anion radical (O2•−) formed in the xanthine/xanthine oxidase enzymatic system. They also reduced the oxoferryl form of the heme group formed in the reaction of myoglobin with t-BOOH. DNICs associated with serum albumin were found to be rapidly destroyed in a model system containing metmyoglobin and t-BOOH. At the same time, these protein DNICs inhibited the t-BOOH-induced oxidative degradation of coenzymes Q9 and Q10 in rat myocardial homogenate. The possible mechanisms of the antioxidant and antiradical action of the DNICs studied and their role in the metabolism of reactive oxygen and nitrogen species are discussed.
Screening Potato Landraces to Cope with Climate Change in the Central Andes of Peru
Authors: Zevallos Edith , Marmolejo Karina , Alvarez Fernando , Paitan Rocio , Viza Ines , Becerra Dante , Rixi Gina , Silva-Diaz Cecilia , Inga Josue
Published: December 2023

Agriculture in the Andean region is mainly small-scale and rainfed, especially in Peru where almost 80% of its population depends on agriculture-related activities. Climate change in addition to social factors threatens the food security of this region. The forecast of more frequent dry spells would especially affect potato crops, domesticated centuries ago in the Andes, where there remains a great genetic diversity. This study aimed to characterize the response to drought stress of 79 potato landraces traditionally grown in the Central Andes of Peru (Pasco region) as a first selection for future breeding evaluations. The used indices were mean productivity (MP), geometric mean productivity (GMP), stress tolerance (STI), stress susceptibility (SSI), and tolerance index (TOL), and a scoring methodology that integrates all of them into a single descriptor in a simple and fast way. The varied responses showed a wide genetic diversity within the assessed landraces, where at least nine of them own high resilience and productivity qualities, and many others are highly vulnerable to drought. It is recommended to complement these studies with physiological and molecular evaluations in stress situations, especially in those with tolerance qualities highlighted in this study, and thus promote the conservation of the biodiversity of this region.
Functional Identification of Olfactory Receptors of Cnaphalocrocis medinalis (Lepidoptera: Crambidae) for Plant Odor
Authors: Cheng Jianjun , Gui Jiawei , Yao Xiaoming , Zhao Hong , Zhou Yujie , Du Yongjun
Published: December 2023

Cnaphalocrocis medinalis (Lepidoptera: Crambidae) is a migratory insect pest on rice crops. The migratory C. medinalis population in a particular location may be immigrants, local populations, emigrants, or a mix of these. Immigrants are strongly attracted to plant odor. We conducted research to identify the olfactory receptors in a floral scent mixture that is strongly attractive to C. medinalis. Through gene cloning, 12 olfactory receptor (OR) genes were amplified and expressed in Xenopus oocytes in vitro, and three of them were found to be responsive to plant foliar and floral volatiles. These were CmedOR31, a specific receptor for geraniol; CmedOR32, a broad-spectrum OR gene that responded to both foliar and floral odors; and CmedOR1, which strongly responded to 10−4 M phenylacetaldehyde. The electrophysiological response to phenylacetaldehyde was extremely high, with a current of 3200 ± 86 nA and an extremely high sensitivity. We compared the phylogenetic tree and sequence similarity of CmedOR genes and found that CmedOR1 belonged to a uniquely conserved OR pedigree in the evolution of Glossata species, and the ORs of this pedigree strongly responded to phenylacetaldehyde. The expression of OR1 was significantly higher in the females than in the males. Localization of CmedOR1 in the antennae of C. medinalis by fluorescence in situ hybridization showed that CmedOR1 was expressed in both males and females. CmedOR1 may be an odor receptor used by females to locate food sources. The function of these ORs and their role in pest monitoring were discussed.
Fine Structure of the Mouthparts of Three Tomicus Beetles Co-Infecting Pinus yunnanensis in Southwestern China with Some Functional Comments
Authors: Cui Yajie , Zhang Mengdie , Zhu Haidi , Yang Pei , Yang Bin , Li Zongbo
Published: December 2023

Tomicus yunnanensis, T. brevipilosus, and T. minor are the most economically significant pests of Pinus yunnanensis in Southwestern China. Chemical and physical factors play critical roles in diverse biological activities. Here, we describe the fine structure of the adult mouthparts of these three Tomicus species using scanning and transmission electron microscopy. We identified three types of mandibular shapes, which determine their biomechanical properties, their ability to process food, and their preferred foraging locations on tree trunks. Eleven types of sensilla were discernible, including sensilla basiconica (Sb.1–2), sensilla twig basiconica (Stb.1–3), sensilla coeloconica (Sco), sensilla chaetica (Sch.1–2), sensilla trichoidea (Str.1–2), and sensilla digitiformia (Sdi). Each basiconic sensillum occurs on the palpal tips and is innervated by 2–6 dendrites. Sb.1 are gustatory receptors, Sb.2 are olfactory receptors, and the three other sensilla have dual taste and mechanical functions. Sco, Sch, and Str are mechanoreceptors. Sdi are mechanical vibration receptions, given that they are innervated by one dendrite with numerous dendritic branches into the nonporous cuticle. No significant differences among the sexes or species were identified; however, intraspecific variability in the number of Stb.3 and Sdi sensilla was evident. These results will aid future studies of Tomicus beetle behaviors.
Shear Wave Elastography for Assessing Liver Stiffness in HCV-Infected Kidney Transplant Recipients after Direct-Acting Antiviral Treatment: A Comparative Study with Magnetic Resonance Elastography
Authors: Almutawakel Salma , Halleck Fabian , Dürr Michael , Grittner Ulrike , Schrezenmeier Eva , Budde Klemens , Althoff Christian E. , Hamm Bernd , Sack Ingolf , Fischer Thomas , Marticorena Garcia Stephan R.
Published: December 2023

Hepatitis C virus (HCV) infection can lead to hepatic fibrosis. The advent of direct-acting antivirals (DAAs) has substantially improved sustained virological response (SVR) rates. In this context, kidney transplant recipients (KTRs) are of particular interest due to their higher HCV infection rates and uncertain renal excretion and bioavailability of DAAs. We investigated liver stiffness after DAA treatment in 15 HCV-infected KTRs using ultrasound shear wave elastography (SWE) in comparison with magnetic resonance elastography (MRE). KTRs were treated with DAAs (daclatasvir and sofosbuvir) for three months and underwent SWE at baseline, end of therapy (EOT), and 3 (EOT+3) and 12 months (EOT+12) after EOT. Fourteen patients achieved SVR12. Shear wave speed (SWS)—as a surrogate parameter for tissue stiffness—was substantially lower at all three post-therapeutic timepoints compared with baseline (EOT: −0.42 m/s, p < 0.01; CI = −0.75–−0.09, EOT+3: −0.43 m/s, p < 0.01; CI = −0.75–−0.11, and EOT+12: −0.52 m/s, p < 0.001; CI = −0.84–−0.19), suggesting liver regeneration after viral eradication and end of inflammation. Baseline SWS correlated positively with histopathological fibrosis scores (r = 0.48; CI = −0.11–0.85). Longitudinal results correlated moderately with APRI (r = 0.41; CI = 0.12–0.64) but not with FIB-4 scores (r = 0.12; CI = −0.19–0.41). Although higher on average, SWE-derived measurements correlated strongly with MRE (r = 0.64). In conclusion, SWE is suitable for non-invasive therapy monitoring in KTRs with HCV infection.
Sleep and Postpartum Psychosis: A Narrative Review of the Existing Literature
Authors: Carr Camilla , Borges Daniela , Lewis Katie , Heron Jessica , Wilson Sally , Broome Matthew R. , Jones Ian , Di Florio Arianna , Morales-Muñoz Isabel
Published: December 2023

Sleep problems are extremely common during the postpartum period. The role of sleep in the development of postpartum psychosis (PP) is, however, still under-researched. This narrative review aims to (1) provide a summary of the existing evidence for the associations between sleep problems and PP, (2) discuss the relevant risk factors associated with sleep problems and PP, and (3) suggest future lines of research in this area. Some of the existing literature suggests an association between sleep problems, specifically insomnia, sleep loss and sleep disruption during pregnancy and postpartum, and PP, with the most relevant risk factors including history of bipolar disorder and time of delivery. However, it is still unclear whether the previously mentioned sleep problems are a symptom of, or a trigger for PP. Thus, further research is needed to identify the specific role of sleep problems in PP, using longitudinal designs and more objective measures of sleep. This will allow appropriate detection, intervention and support for women experiencing and/or at risk for PP.
Predictive Factors of Response to Streptozotocin in Neuroendocrine Pancreatic Neoplasms
Authors: Fanciulli Giuseppe , La Salvia Anna , Di Molfetta Sergio , Cannavale Giuseppe , Puliani Giulia , Verrico Monica , Campolo Federica , Colao Annamaria , Faggiano Antongiulio ,   NIKE Group
Published: December 2023

Pancreatic neuroendocrine neoplasms (Pan-NENs) may exhibit a heterogeneous clinical course, ranging from indolent to progressive/metastatic behavior. In the latter scenario, streptozocin (STZ) is considered the cornerstone of systemic treatment; however, response to STZ-based chemotherapy may vary among individuals. In this narrative review, we aimed to identify the predictive factors of response to STZ in advanced Pan-NENs. We performed an extensive search in international online databases for published studies and ongoing clinical trials evaluating STZ in Pan-NENs. We found 11 pertinent studies evaluating 17 patient-, tumor-, or treatment-related factors. Age, CgA blood levels, tumor grade, Ki-67% index, anatomical location of the primary tumor, tumor stage, site of metastasis origin, liver tumor burden, extrahepatic spread, functional status, O6-methylguanine-methyltransferase (MGMT) status, line of therapy, and response to previous treatments were all statistically associated with radiological response and/or survival. The identified predictors may help clinicians make appropriate treatment decisions, in this way improving clinical outcomes in patients with advanced Pan-NENs.
Status after Hospital Discharge: An Observational Study of the Progression of Patients’ Mental Health Symptoms Six Weeks after Hospital Discharge
Authors: Mao Wanying , Shalaby Reham , Owusu Ernest , Elgendy Hossam , Shalaby Nermin , Agyapong Belinda , Nichols Angel , Eboreime Ejemai , Nkire Nnamdi , Agyapong Vincent I. O.
Published: December 2023

(1) Background: Transitioning from mental health inpatient care to community care is often a vulnerable time in the treatment process where additional risks and anxiety may arise. We collected data for this study as part of a pragmatic cluster-randomized, longitudinal approach in Alberta. As the first phase of the ongoing innovative supportive program, this paper assessed the progression of mental health symptoms in patients six weeks after hospital discharge. Factors that may contribute to the presence or absence of anxiety and depression symptoms, as well as well-being, following return to the community were investigated. This provides evidence and baseline data for future phases of the project. (2) Methods: An observational study design was adopted for this study. Data on a variety of sociodemographic and clinical factors were collected at discharge and six weeks after via REDCap. Anxiety, depression, and well-being symptoms were assessed using the Generalized Anxiety Disorder (GAD-7) questionnaire, the Patient Health Questionnaire-9 (PHQ-9), and the World Health Organization-Five Well-Being Index (WHO-5), respectively. Descriptive, chi-square, independent t-tests, and multivariate regression analyses were conducted. (3) Result: The survey was completed by 88 out of 306 participants (28.8% response rate). The chi-square/Fisher exact test and independent t-test revealed no significant change in the mental health conditions from baseline to six weeks after discharge. It was found that the only significant factor predicting symptoms six weeks after discharge from inpatient treatment was the baseline symptoms in all three logistic regression models. It was four times more likely for those who experienced anxiety and depression at baseline to experience anxiety and depression symptoms six weeks after discharge (OR = 4.27; 95% CI: 1.38–13.20) (OR = 4.04; 95% CI: 1.25–13.05). Those with poor baseline well-being were almost 12 times more likely to experience poor well-being six weeks after discharge (OR = 11.75; 95% CI: 3.21–42.99). (4) Conclusions: Study results found no significant change in mental health conditions in the short term following hospital discharge. It is essential that researchers and policymakers collaborate in order to implement effective interventions to support and maintain the mental health conditions of patients following discharge.
Enhancing Underwater Robot Manipulators with a Hybrid Sliding Mode Controller and Neural-Fuzzy Algorithm
Authors: Pham Duc-Anh , Han Seung-Hun
Published: December 2023

The sliding mode controller stands out for its exceptional stability, even when the system experiences noise or undergoes time-varying parameter changes. However, designing a sliding mode controller necessitates precise knowledge of the object’s exact model, which is often unattainable in practical scenarios. Furthermore, if the sliding control law’s amplitude becomes excessive, it can lead to undesirable chattering phenomena near the sliding surface. This article presents a new method that uses a special kind of computer program (Radial Basis Function Neural Network) to quickly calculate complex relationships in a robot’s control system. This calculation is combined with a technique called Sliding Mode Control, and Fuzzy Logic is used to measure the size of the control action, all while making sure the system stays stable using Lyapunov stability theory. We tested this new method on a robot arm that can move in three different ways at the same time, showing that it can handle complex, multiple-input, multiple-output systems. In addition, applying LPV combined with Kalman helps reduce noise and the system operates more stably. The manipulator’s response under this controller exhibits controlled overshoot (Rad), with a rise time of approximately 5 ± 3% seconds and a settling error of around 1%. These control results are rigorously validated through simulations conducted using MATLAB/Simulink software version 2022b. This research contributes to the advancement of control strategies for robotic manipulators, offering improved stability and adaptability in scenarios where precise system modeling is challenging.
Metabolic Regulation of Two pksCT Gene Transcripts in Monascus ruber Impacts Citrinin Biosynthesis
Authors: He Yi , Zhu Lisha , Dong Xingxing , Li Aoran , Xu Suyin , Wang Liling , Shao Yanchun
Published: December 2023

Citrinin (CIT), a secondary metabolite produced by the filamentous fungi Monascus species, exhibits nephrotoxic, hepatotoxic, and carcinogenic effects in mammals, remarkably restricting the utilization of Monascus-derived products. CIT synthesis is mediated through the pksCT gene and modified by multiple genetic factors. Here, the regulatory effects of two pksCT transcripts, pksCTα, and pksCTβ, generated via pre-mRNA alternative splicing (AS), were investigated using hairpin RNA (ihpRNA) interference, and their impact on CIT biosynthesis and the underlying mechanisms were assessed through chemical biology and transcriptome analyses. The CIT yield in ihpRNA-pksCTα and ihpRNA-pksCT (α + β) transformants decreased from 7.2 μg/mL in the wild-type strain to 3.8 μg/mL and 0.08 μg/mL, respectively. Notably, several genes in the CIT biosynthetic gene cluster, specifically mrl3, mrl5, mrr1, and mrr5 in the ihpRNA-pksCT (α + β) transformant, were downregulated. Transcriptome results revealed that silencing pksCT has a great impact on carbohydrate metabolism, amino acid metabolism, lipid metabolism, and AS events. The key enzymes in the citrate cycle (TCA cycle) and glycolysis were significantly inhibited in the transformants, leading to a decrease in the production of biosynthetic precursors, such as acetyl-coenzyme-A (acetyl-coA) and malonyl-coenzyme-A (malonyl-coA). Furthermore, the reduction of CIT has a regulatory effect on lipid metabolism via redirecting acetyl-coA from CIT biosynthesis towards lipid biosynthesis. These findings offer insights into the mechanisms underlying CIT biosynthesis and AS in Monascus, thus providing a foundation for future research.
ECB Monetary Policy and the Term Structure of Bank Default Risk
Authors: Beernaert Tom , Soenen Nicolas , Vander Vennet Rudi
Published: December 2023

Euro Area banks have been confronted with unprecedented monetary policy actions by the ECB. Monetary policy may affect bank risk profiles, but the consequences may differ for short-term risk versus long-term or structural bank risk. We empirically investigated the association between the ECB’s monetary policy stance and market-perceived short-term and long-term bank risk, using the term structure of default risk captured by bank CDS spreads. The results demonstrated that, during the period 2009–2020, ECB expansionary monetary policy diminished bank default risk in the short term. However, we did not observe a similar decline in long-term bank default risk, since we documented that monetary stimulus is associated with a steepening of the bank default risk curve. The reduction of bank default risk was most pronounced during the sovereign debt crisis and for periphery Euro Area banks. From 2018 onwards, monetary policy accommodation is associated with increased bank default risk, both short-term and structurally, which is consistent with the risk-taking hypothesis under which banks engage in excessive risk-taking behavior in their loan and securities portfolios to compensate profitability pressure caused by persistently low rates. The increase in perceived default risk is especially visible for banks with a high reliance on deposit funding.
Choice of Healing Agent for Self-Healing Asphalt Concrete
Authors: Inozemtcev Sergei Sergeevich , Korolev Evgeniy Valerievich , Do Trong Toan
Published: December 2023

The choice of a substance as a healing agent for asphalt concrete is determined by the scientific experience of researchers and the results of exploratory studies. There are no standard approaches for selecting healing agents or assessing their compatibility with the matrix components in asphalt concrete. However, such methods would make it possible to systematize research in the field of self-healing asphalt concrete and significantly expand the list of healing agents potentially suitable for encapsulation and ensuring the formation of a healing effect. An approach has been proposed for studying a substance and assessing the suitability of its use as a healing agent during encapsulation, using alginate technology in terms of solubility, homogeneity in a system with bitumen, and adhesive strength. This set of indicators can be used in the development and design of self-healing asphalt concrete, as well as for expanding the list of healing agents that can be used to implement self-healing technology. This article discusses sunflower oil and AR polymers as healing agents for self-healing asphalt concretes. The substances under consideration are capable of forming a homogeneous system ∆δ → 0 with bitumen, and the double systems “SfO-bitumen” and “ARP-bitumen” have a Gibbs energy value ∆G < 0, which confirms this. The studied healing agents are able to form an emulsion in alginate aqueous solutions, which was confirmed by the structuring effect and the extreme influence of their concentration on viscosity. The strength of the adhesive bonds under the influence of SfO was 14.2% of the initial value of the tensile strength during splitting. Under the influence of ARP, the strength of the adhesive bonds was 5.8% of the initial value of the tensile strength at splitting. The use of an activator in asphalt concrete makes it possible to increase the strength of the adhesive bonds to 25–45% of the initial splitting strength.
Organic and Biogenic Nanocarriers as Bio-Friendly Systems for Bioactive Compounds’ Delivery: State-of-the Art and Challenges
Authors: Petrovic Sanja M. , Barbinta-Patrascu Marcela-Elisabeta
Published: December 2023

“Green” strategies to build up novel organic nanocarriers with bioperformance are modern trends in nanotechnology. In this way, the valorization of bio-wastes and the use of living systems to develop multifunctional organic and biogenic nanocarriers (OBNs) have revolutionized the nanotechnological and biomedical fields. This paper is a comprehensive review related to OBNs for bioactives’ delivery, providing an overview of the reports on the past two decades. In the first part, several classes of bioactive compounds and their therapeutic role are briefly presented. A broad section is dedicated to the main categories of organic and biogenic nanocarriers. The major challenges regarding the eco-design and the fate of OBNs are suggested to overcome some toxicity-related drawbacks. Future directions and opportunities, and finding “green” solutions for solving the problems related to nanocarriers, are outlined in the final of this paper. We believe that through this review, we will capture the attention of the readers and will open new perspectives for new solutions/ideas for the discovery of more efficient and “green” ways in developing novel bioperformant nanocarriers for transporting bioactive agents.
An Overview on Fatigue of High-Entropy Alloys
Authors: Hu Junchao , Li Xue , Zhao Qiuchen , Chen Yangrui , Yang Kun , Wang Qingyuan
Published: December 2023

Due to their distinct physical, chemical, and mechanical features, high-entropy alloys have significantly broadened the possibilities of designing metal materials, and are anticipated to hold a crucial position in key engineering domains such as aviation and aerospace. The fatigue performance of high-entropy alloys is a crucial aspect in assessing their applicability as a structural material with immense potential. This paper provides an overview of fatigue experiments conducted on high-entropy alloys in the past two decades, focusing on crack initiation behavior, crack propagation modes, and fatigue life prediction models.
Modal Balancing of Warped Rotors without Trial Runs Using the Numerical Assembly Technique
Authors: Quinz Georg , Überwimmer Gregor , Klanner Michael , Ellermann Katrin
Published: December 2023

The increasing use of high-speed machinery leads to a growing demand for efficient balancing methods for flexible rotors. Conventional balancing methods are costly and time-consuming since they require multiple trial runs. For this reason, recent research focuses on model-based balancing methods, which substitute measurements with simulations. This work presents and examines a model-based modal balancing method, which utilizes the Numerical Assembly Technique (NAT) for the in situ balancing of warped rotors with flexible behaviour. NAT is a successive modification of discrete–continuous modelling that leads to analytical harmonic solutions and is very computationally efficient. In this version of NAT, internal damping is also included with a viscoelastic material model using fractional time derivatives. The modal balancing procedure is adapted to handle measurements outside of the critical speeds and the effect of the pre-bend on the rotor. The accuracy of the simulations is shown by comparing measured mode shapes and eigenvalues with values calculated with NAT. Furthermore, the first two modes of a rotor test bed are successfully balanced without trial runs.
Dimethyl Bisphenolate Ameliorates Carbon Tetrachloride-Induced Liver Injury by Regulating Oxidative Stress-Related Genes
Authors: Wang Rong , Shen Huanhuan , Zhang Jiaxiang , Li Xiyan , Guo Yang , Zhao Zhenjun , Wang Pingyu , Xie Ning , Li Youjie , Qu Guiwu , Xie Shuyang
Published: December 2023

Liver disease accounts for millions of deaths per year all over the world due to complications from cirrhosis and liver injury. In this study, a novel compound, dimethyl bisphenolate (DMB), was synthesized to investigate its role in ameliorating carbon tetrachloride (CCl4)-induced liver injury through the regulation of oxidative stress-related genes. The structure of DMB was confirmed based on its hydrogen spectrum and mass spectrometry. DMB significantly reduced the high levels of ALT, AST, DBIL, TBIL, ALP, and LDH in a dose-dependent manner in the sera of CCl4-treated rats. The protective effects of DMB on biochemical indicators were similar to those of silymarin. The ROS fluorescence intensity increased in CCl4-treated cells but significantly weakened in DMB-treated cells compared with the controls. DMB significantly increased the content of oxidative stress-related GSH, Nrf2, and GCLC dose-dependently but reduced MDA levels in CCl4-treated cells or the liver tissues of CCl4-treated rats. Moreover, DMB treatment decreased the expression levels of P53 and Bax but increased those of Bcl2. In summary, DMB demonstrated protective effects on CCl4-induced liver injury by regulating oxidative stress-related genes.
Analysis of Differences in the Chemical Composition of Glycosides and Sugars between Four Forms of Fresh Rehmanniae Radix
Authors: Xu Lu , Guo Xiaokai , Xue Shujuan , Di Ruiyi , Chen Suiqing
Published: December 2023

Fresh Rehmanniae Radix, as well as its processed products, are widely used in the clinical practice of traditional Chinese medicine. It is mainly available in four forms: fresh Rehmanniae Radix, raw Rehmanniae Radix, prepared Rehmanniae Radix, and nine-steamed, nine-dried Rehmanniae Radix. Pharmacological studies have shown that all Rehmanniae Radix forms contain iridoid glycosides and sugar compounds with various effects, including hypoglycemic, anti-inflammatory, neuroprotective, immunological enhancement, and bone marrow hematopoiesis-promoting activities. Differences in the efficacy among these Rehmanniae Radix forms and their processed products have been attributed to variations in their chemical compositions, particularly in iridoid glycosides and sugar compounds; however, the specific compositional differences in glycosides and sugars among the four forms of Rehmanniae Radix have not been clarified. Therefore, this study aims to qualitatively characterize the iridoid glycosides and sugar compounds in fresh Rehmanniae Radix, raw Rehmanniae Radix, prepared Rehmanniae Radix, and nine-steamed, nine-dried Rehmanniae Radix.
Insight into the Inhibitory Mechanism of Embryonic Ectoderm Development Subunit by Triazolopyrimidine Derivatives as Inhibitors through Molecular Dynamics Simulation
Authors: Ju Jianan , Zhang Hao , Guan Shanshan , Liu Chang , Du Juan , Shen Xiaoli , Wang Song
Published: December 2023

Inhibition of the Embryonic Ectoderm Development (EED) subunit in Polycomb Repressive Complex 2 (PRC2) can inhibit tumor growth. In this paper, we selected six experimentally designed EED competitive Inhibitors of the triazolopyrimidine derivatives class. We investigated the difference in the binding mode of the natural substrate to the Inhibitors and the effects of differences in the parent nuclei, heads, and tails of the Inhibitors on the inhibitory capacity. The results showed that the binding free energy of this class of Inhibitors was close to or lower compared to the natural substrate, providing an energetic basis for competitive inhibition. For the Inhibitors, the presence of a strong negatively charged group at the 6-position of the parent nucleus or the 8′-position of the head would make the hydrogen atom on the head imino group prone to flip, resulting in the vertical movement of the parent nucleus, which significantly decreased the inhibitory ability. When the 6-position of the parent nucleus was a nonpolar group, the parent nucleus would move horizontally, slightly decreasing the inhibitory ability. When the 8′-position of the head was methylene, it formed an intramolecular hydrophobic interaction with the benzene ring on the tail, resulting in a significant increase in inhibition ability.
Quantitatively Exploring Giant Optical Anisotropy of Quasi-One-Dimensional Ta2NiS5
Authors: Zhang Qihang , Gu Honggang , Guo Zhengfeng , Ding Ke , Liu Shiyuan
Published: December 2023

Optical anisotropy offers a heightened degree of flexibility in shaping optical properties and designing cutting-edge devices. Quasi-one-dimensional Ta2NiS5, with giant optical anisotropy, has been used in the development of new lasers and sensors. In this research endeavor, we successfully acquired the complete dielectric tensor of Ta2NiS5, utilizing the advanced technique of Mueller matrix spectroscopic ellipsometry, enabling a rigorous quantitative assessment of its optical anisotropy. The results indicate that Ta2NiS5 demonstrates giant birefringence and dichroism, with Δnmax = 1.54 and Δkmax = 1.80. This pursuit also delves into the fundamental underpinnings of this optical anisotropy, drawing upon a fusion of first-principles calculations and critical points analysis. The anisotropy of Ta2NiS5 arises from differences in optical transitions in different directions and is shown to be due to van Hove singularities without exciton effects. Its giant optical anisotropy is expected to be useful in the design of novel optical devices, and the revelation of the physical mechanism facilitates the modulation of its optical properties.
Breastfeeding Duration Is Associated with the Risk of Tooth Loss, Chewing Difficulty, and Undernutrition among Older Korean Women: Results of the Korea National Health and Nutrition Examination Survey (KNHANES) 2013–2015
Authors: Jo Ye Rang , Park Yoo Kyoung , Lim Hee-Sook
Published: December 2023

We investigated whether older Korean women with prolonged breastfeeding duration have an increased risk of tooth loss, chewing difficulty, or undernutrition, as well as whether tooth loss and chewing difficulty mediate the association between breastfeeding duration and undernutrition risk. This study included 1666 women aged ≥65 years from the 2013–2015 Korea National Health and Nutrition Examination Survey who breastfed after delivery. The number of teeth and chewing ability were investigated based on the status of individual teeth and a self-report questionnaire, respectively. Dietary intake was estimated using the 24 h recall method. Compared with women who breastfed for 1–18 months, the odds ratios for tooth loss were 1.16 (95% confidence interval [CI] = 0.69–1.94), 1.79 (95% CI = 1.08–2.94), and 1.86 (95% CI = 1.16–2.97) among women who breastfed for 19–36, 37–72, and ≥73 months, respectively (p for trend = 0.004). Similar results were obtained for chewing difficulty and undernutrition. Furthermore, tooth loss and chewing difficulty partially mediated the association between breastfeeding duration and undernutrition risk. In conclusion, older Korean women who breastfed for longer periods are more likely to experience tooth loss, chewing difficulty, and undernutrition, which are particularly severe among women who breastfed for ≥37 months. The association between breastfeeding duration and undernutrition risk is mediated by tooth loss and chewing difficulty.
Metabolomic Profiling of Obese Patients with Altered Intestinal Permeability Undergoing a Very Low-Calorie Ketogenic Diet
Authors: Calabrese Francesco Maria , Celano Giuseppe , Riezzo Giuseppe , D’Attoma Benedetta , Ignazzi Antonia , Di Chito Martina , Sila Annamaria , De Nucci Sara , Rinaldi Roberta , Linsalata Michele , Vacca Mirco , Apa Carmen Aurora , De Angelis Maria , Giannelli Gianluigi , De Pergola Giovanni , Russo Francesco
Published: December 2023

A healthy intestinal permeability facilitates the selective transport of nutrients, metabolites, water, and bacterial products, involving cellular, neural, hormonal, and immune factors. An altered intestinal permeability indicates pathologic phenotypes and is associated with the exacerbation of obesity and related comorbidities. To investigate the impact of altered permeability in obese patients undergoing a calorie-restrictive dietary regimen (VLCKD), we collected urinary and fecal samples from obese patients with both normal and altered permeability (determined based on the lactulose/mannitol ratio) before and after treatment. The analysis of volatile organic compounds (VOCs) aids in understanding the metabolites produced by the intestinal microbiota in this unique ecological niche. Furthermore, we examined clinical and anthropometric variables from the cohort and compared them to significant VOC panels. Consequently, we identified specific markers in the metabolomics data that differentiated between normal and altered profiles before and after the diet. These markers indicated how the variable contribution specifically accounted for interleukins and lipopolysaccharides (LPS). The targeted metabolomics experiment detected no differences in measured short-chain fatty acids (SCFA). In summary, our study evaluated metabolomic markers capable of distinguishing low-grade inflammation conditions, exacerbated in more advanced stages of obesity with altered intestinal permeability.
Herpesvirus Diseases in Humans and Animals: Recent Developments, Challenges, and Charting Future Paths
Authors: Šudomová Miroslava , Hassan Sherif T. S.
Published: December 2023

Herpesviruses, a family of enveloped DNA viruses, pose significant threats to both humans and animals [...]
Utility of Feathers for Avian Influenza Virus Detection in Commercial Poultry
Authors: Azeem Shahan , Guo Baoqing , Sato Yuko , Gauger Phillip C. , Wolc Anna , Yoon Kyoung-Jin
Published: December 2023

The present study evaluated the potential utility of feather samples for the convenient and accurate detection of avian influenza virus (AIV) in commercial poultry. Feather samples were obtained from AIV-negative commercial layer facilities in Iowa, USA. The feathers were spiked with various concentrations (106 to 100) of a low pathogenic strain of H5N2 AIV using a nebulizing device and were evaluated for the detection of viral RNA using a real-time RT-PCR assay immediately or after incubation at −20, 4, 22, or 37 °C for 24, 48, or 72 h. Likewise, cell culture medium samples with and without the virus were prepared and used for comparison. In the spiked feathers, the PCR reliably (i.e., 100% probability of detection) detected AIV RNA in eluates from samples sprayed with 103 EID50/mL or more of the virus. Based on half-life estimates, the feathers performed better than the corresponding media samples (p < 0.05), particularly when the samples were stored at 22 or 37 °C. In conclusion, feather samples can be routinely collected from a poultry barn as a non-invasive alternative to blood or oropharyngeal–cloacal swab samples for monitoring AIV.
Body Surface Area-Based Dosing of Mycophenolate Mofetil in Pediatric Hematopoietic Stem Cell Transplant Recipients: A Prospective Population Pharmacokinetic Study
Authors: Park Hyun Jin , Hong Kyung Taek , Han Nayoung , Kim In-Wha , Oh Jung Mi , Kang Hyoung Jin
Published: December 2023

Mycophenolate mofetil (MMF) is commonly used for acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (HSCT). However, limited population pharmacokinetic (PPK) data are available for pediatric HSCT patients. This study aimed to develop a PPK model and recommend optimal oral MMF dosage in pediatric HSCT patients. This prospective study involved pediatric HSCT patients at a tertiary academic institution. Patients received oral MMF 15–20 mg/kg twice daily for aGVHD prophylaxis and treatment. The PPK analysis was conducted using a nonlinear mixed-effects modeling method. Simulation was performed considering different body surface areas (BSAs) (0.5 m2, 1.0 m2, 1.5 m2) and dosing (400 mg/m2, 600 mg/m2, 900 mg/m2 twice daily). Based on the simulation, an optimal dosage of oral MMF was suggested. A total of 20 patients and 80 samples were included in the PPK model development. A one-compartment model with first-order absorption adequately described the pharmacokinetics of mycophenolic acid (MPA). BSA was a statistically significant covariate on Vd/F. Simulation suggested the optimal dosage of oral MMF as 900 mg/m2 twice daily, respectively. A reliable PPK model was developed with good predictive performance. This model-informed optimal MMF dosage in pediatric HSCT patients can provide valuable dosing guidance in real-world clinical practice.
Combined Radiation and Temperature Effects on Brillouin-Based Optical Fiber Sensors
Authors: Perrot Jérémy , Morana Adriana , Marin Emmanuel , Ouerdane Youcef , Boukenter Aziz , Bertrand Johan , Girard Sylvain
Published: December 2023

The combined effects of temperature (from −80 °C to +80 °C) and 100 kV X-ray exposure (up to 108 kGy(SiO2)) on the physical properties of Brillouin scattering and losses in three differently doped silica-based optical fibers, with varying dopant type and concentration (4 wt%(Ge), 10 wt%(Ge) and 1 wt%(F)), are experimentally studied in this work. The dependencies of Brillouin Frequency Shifts (BFS), Radiation-Induced Attenuation (RIA) levels, Brillouin gain attenuation, Brillouin frequency temperature (CT) and strain (Cε) sensitivity coefficients are studied under X-rays in a wide temperature range [−80 °C; +80 °C]. Brillouin sensing capabilities are investigated using a Brillouin Optical Time Domain Analyzer (BOTDA), and several properties are reported: (i) similar behavior of the Brillouin gain amplitude decrease with the increase in the RIA; (ii) the F-doped and heavily Ge-doped fibers do not exhibit a temperature dependence under radiation for their responses in Brillouin gain losses. Increasing Ge dopant concentration also reduces the irradiation temperature effect on RIA. In addition, Radiation-Induced Brillouin Frequency Shift (RI-BFS) manifests a slightly different behavior for lower temperatures than RIA, presenting an opportunity for a comprehensive understanding of RI-BFS origins. Related temperature and strain sensors are designed for harsh environments over an extended irradiation temperature range, which is useful for a wide range of applications.
Association Mapping for Evaluation of Population Structure, Genetic Diversity, and Physiochemical Traits in Drought-Stressed Maize Germplasm Using SSR Markers
Authors: Ilyas Muhammad Zahaib , Park Hyeon , Jang So Jung , Cho Jungeun , Sa Kyu Jin , Lee Ju Kyong
Published: December 2023

Globally, maize is one of the most consumed crops along with rice and wheat. However, maize is sensitive to different abiotic stress factors, such as drought, which have a significant impact on its production. The aims of this study were to investigate (1) genetic variation among 41 maize-inbred lines and the relationships among them and (2) significant marker–trait associations (SMTAs) between 7 selected physiochemical traits and 200 simple sequence repeat (SSR) markers to examine the genetics of these traits. A total of 1023 alleles were identified among the 41 maize-inbred lines using the 200 SSR loci, with a mean of 5.1 alleles per locus. The average major allele frequency, gene diversity, and polymorphism information content were 0.498, 0.627, and 0.579, respectively. The population structure analysis based on the 200 SSR loci divided the maize germplasm into two primary groups with an admixed group. Moreover, this study identified, respectively, 85 SMTAs and 31 SMTAs using a general linear model (Q GLM) and a mixed linear model (Q  + K MLM) with statistically significant (p < 0.05 and <0.01) associations with the seven physiochemical traits (caffeic acid content, chlorogenic acid content, gallic acid content, ferulic acid content, 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity, leaf relative moisture content, total phenolic content). These SSR markers were highly correlated with one or more of the seven physiochemical traits. This study provides insights into the genetics of the 41 maize-inbred lines and their seven physiochemical traits and will be of assistance to breeders in the marker-assisted selection of maize for breeding programs.
Recent Advances in the Integrative Taxonomy of Plants
Authors: Maltsev Yevhen , Erst Andrey
Published: December 2023

Biodiversity conservation and management call for rapid and accurate global assessments at the species level [...]
Hydrangea arborescens ‘Annabelle’ Flower Formation and Flowering in the Current Year
Authors: Huang Xiaoxu , Lyu Tong , Li Zheng , Lyu Yingmin
Published: December 2023

The perennial woody plant Hydrangea arborescens ‘Annabelle’ is of great research value due to its unique mechanism of flower development that occurs in the current year, resulting in decorative flowers that can be enjoyed for a relatively long period of time. However, the mechanisms underlying the regulation of current-year flower development in H. arborescens ‘Annabelle’ are still not fully understood. In this study, we conducted an associated analysis to explore the core regulating network in H. arborescens ‘Annabelle’ by combining phenological observations, physiological assays, and transcriptome comparisons across seven flower developmental stages. Through this analysis, we constructed a gene co-expression network (GCN) based on the highest reciprocal rank (HRR), using 509 differentially expressed genes (DEGs) identified from seven flowering-related pathways, as well as the biosynthesis of eight flowering-related phytohormones and signal transduction in the transcriptomic analysis. According to the analysis of the GCN, we identified 14 key genes with the highest functional connectivity that played critical roles in specific development stages. We confirmed that 135 transcription factors (AP2/ERF, bHLH, CO-like, GRAS, MIKC, SBP, WRKY) were highly co-expressed with the 14 key genes, indicating their close associations with the development of current-year flowers. We further proposed a hypothetical model of a gene regulatory network for the development of the whole flower. This model suggested that the photoperiod, aging, and gibberellin pathways, along with the phytohormones abscisic acid (ABA), gibberellin (GA), brassinosteroid (BR), and jasmonic acid (JA), work synergistically to promote the floral transition. Additionally, auxin, GA, JA, ABA, and salicylic acid (SA) regulated the blooming process by involving the circadian clock. Cytokinin (CTK), ethylene (ETH), and SA were key regulators that affected flower senescence. Additionally, several floral integrators (HaLFY, HaSOC1-2, HaAP1, HaFULL, HaAGL24, HaFLC, etc.) were dominant contributors to the development of H. arborescens flowers. Overall, this research provides a comprehensive understanding of the dynamic mechanism underlying the entire process of current-year flower development, thereby offering valuable insights for further studies on the flower development of H. arborescens ‘Annabelle’.