bluuebunny/crossref_metadata_2025 huggingface dataset:
Consistency of P53 immunohistochemical expression between preoperative biopsy and final surgical specimens of endometrial cancer
Authors: Zhang Jun , Jiang Peng , Gong Chunxia , Kong Wei , Tu Yuan , Huang Yuzhen , Liu Ying
Published: August 2023

Objective

The aim of this study is to explore the consistency of P53 immunohistochemical expression between preoperative biopsy and final pathology in endometrial cancer (EC), and to predict the prognosis of patients based on the 4-tier P53 expression and classic clinicopathological parameters.

Methods

The medical data of patients with stage I-III EC who received preoperative biopsy and initial surgical treatment in two medical centers was retrospectively collected. The consistency of P53 immunohistochemistry expression between preoperative biopsy and final pathology was compared using Cohen’s kappa coefficient and Sankey diagram, then 4-tier P53 expression was defined (P53wt/P53wt, P53abn/P53wt, P53wt/P53abn, and P53abn/P53abn). Univariate and multivariate Cox regression analysis was used to determine the correlation between 4-tier P53 expression and the prognosis of patients. On this basis, the nomogram models were established to predict the prognosis of patients by combining 4-layer P53 expression and classic clinicopathological parameters, then risk stratification was performed on patients.

Results

A total of 1186 patients were ultimately included in this study through inclusion and exclusion criteria. Overall, the consistency of P53 expression between preoperative biopsy and final pathology was 83.8%, with a kappa coefficient of 0.624. ROC curve suggested that the AUC of 4-tier P53 expression to predict the prognosis of patients was better than AUC of P53 expression in preoperative biopsy or final pathology alone. Univariate and multivariate Cox regression analysis suggested that 4-tier P53 expression was an independent influencing factor for recurrence and death. On this basis, the nomogram models based on 4-tier P53 expression and classical clinicopathological factors were successfully established. ROC curve suggested that the AUC (AUC for recurrence and death was 0.856 and 0.838, respectively) of the models was superior to the single 4-tier P53 expression or the single classical clinicopathological parameters, which could provide a better risk stratification for patients.

Conclusion

The expression of P53 immunohistochemistry had relatively good consistency between preoperative biopsy and final pathology of EC. Due to the discrepancy of P53 immunohistochemistry between preoperative biopsy and final pathology, the prognosis of patients can be better evaluated based on the 4-layer P53 expression and classic clinical pathological parameters.
Pharmacological action of Hedysarum polysaccharides: a review
Authors: Gao Xiang , Ren Chunzhen , Li Linyu , Zhao Huilin , Liu Kai , Zhuang Mengjie , Lv Xinfang , Zhi Xiaodong , Jiang Hugang , Chen Qilin , Zhao Xinke , Li Yingdong
Published: August 2023

Hedysarum, a traditional Chinese herbal medicine and food with a long history of clinical application, is used to improve health conditions and treat various diseases. Hedysarum polysaccharides (HPS), flavonoids, saponins, and alkaloids, are the primary components of Hedysarum. HPS is the most important natural active ingredient of Hedysarum, which has many pharmacological effects. Currently, HPS exhibits significant promise in drug development for various ailments such as tumors, diabetes, cardiovascular diseases, Alzheimer’s disease, and fibrosis. This review paper discusses the extraction, separation, and content determination techniques of HPS, along with the investigation of its chemical constituents. More importantly, we reviewed the anti-inflammatory pharmacological effects of HPS, such as inhibition of inflammatory factors and NF-κB signaling pathway; antitumor activity through apoptosis induction in tumor cells and blocking tumor cell proliferation and metastasis; antioxidant effects; regulation of various cytokines and immune cells; regulation of blood sugar levels, such as in type I and type II diabetes and in diabetic complications; improvement in symptoms of Alzheimer disease; anti-aging and anti-fibrosis properties; and improvement in cerebral ischemia-reperfusion injury. This review paper establishes the theoretical foundation for future studies on the structure, mechanism, and clinical use of HPS.
Inadequate gastric preparation and its associated factors for magnetically controlled capsule endoscopy
Authors: Kong Qing-Zhou , Peng Cheng , Li Zhen , Tian Bao-Ling , Li Yue-Yue , Chen Fei-Xue , Zuo Xiu-Li , Li Yan-Qing
Published: August 2023

Goals: To explore factors associated with inadequate gastric preparation for MCE.

Background: Factors associated with inadequate gastric preparation for magnetically controlled capsule endoscopy (MCE) remains unclear.

Study: Data of patients who underwent MCE from June 2021 to July 2022 were prospectively collected. The gastric cleanliness score (GCS) of the six stomach regions (gastric cardia, fundus, body, angulus, antrum, and pylorus) was recorded. Patients with GCS score ≥18 were defined as the adequate preparation. Factors related to inadequate gastric preparation were analyzed using a logistic regression model with estimated odds ratios (OR).

Results: The mean GCS score of 211 patients was 17.01 ± 2.82. In the multivariable analysis, proton pump inhibitor (PPI) use (OR 3.57; 95% CI 1.69–7.95; p < 0.01) and premedication time after administering simethicone <30 min (OR 2.86; 95% CI 1.10–7.39; p = 0.03) were independent risk factors for inadequate gastric preparation. Comparing the gastric cleanliness of different locations, the median GCS of the lower stomach [10.00, IQR (9.50, 11.00)] was significantly higher than that of the upper stomach [7.00, IQR (6.00, 8.00)] (p <0.001).

Conclusion: PPI use and inadequate premedication time (<30 min) may reduce the quality of gastric preparation for MCE. The type, dose, duration of medication, and discontinuation time of PPIs was well worth further exploration. Appropriate control of the type and time of premedication may be the key to improving overall gastric cleanliness.
The functions and regulatory pathways of S100A8/A9 and its receptors in cancers
Authors: Zhou Huimin , Zhao Cong , Shao Rongguang , Xu Yanni , Zhao Wuli
Published: August 2023

Inflammation primarily influences the initiation, progression, and deterioration of many human diseases, and immune cells are the principal forces that modulate the balance of inflammation by generating cytokines and chemokines to maintain physiological homeostasis or accelerate disease development. S100A8/A9, a heterodimer protein mainly generated by neutrophils, triggers many signal transduction pathways to mediate microtubule constitution and pathogen defense, as well as intricate procedures of cancer growth, metastasis, drug resistance, and prognosis. Its paired receptors, such as receptor for advanced glycation ends (RAGEs) and toll-like receptor 4 (TLR4), also have roles and effects within tumor cells, mainly involved with mitogen-activated protein kinases (MAPKs), NF-κB, phosphoinositide 3-kinase (PI3K)/Akt, mammalian target of rapamycin (mTOR) and protein kinase C (PKC) activation. In the clinical setting, S100A8/A9 and its receptors can be used complementarily as efficient biomarkers for cancer diagnosis and treatment. This review comprehensively summarizes the biological functions of S100A8/A9 and its various receptors in tumor cells, in order to provide new insights and strategies targeting S100A8/A9 to promote novel diagnostic and therapeutic methods in cancers.
Analysis of 12 cases of antineoplastic agents-induced interstitial lung disease
Authors: Li Xiao , Gu Yong-Li , Liu Xu-Chao , Sun Zeng-Xian , Sun Ying
Published: August 2023

Objective: To summarize the situation of antineoplastic agents-induced interstitial lung diseases (ILD), provide reference for strengthening clinical management of druginduced interstitial lung diseases (DILD).

Methods: We retrospectively investigated the medical records of 12 patients with antineoplastic agents-induced ILD in a hospital between January and December 2020. Data collected included patients’ characteristic (gender, age, ECOG PS score, smoking history, primary tumor, concurrent diseases or complications.) and treatment conditions (DILD-causing drugs, clinical symptoms, chest CT, DILD treatment drugs, onset cycle, onset time, severity of DILD, DILD course and prognosis.).

Results: The median age of 12 DILD cases was 68%, 66.67% of the patients were male, lung cancer accounted for 58.33% (7/12). DILD was induced by cytotoxicity drugs, targeted drugs and immune checkpoint inhibitors (ICIs), of which ICIs accounted for 66.67% (8/12). Scattered patchy, cord-like, grid-like or flocculent shadows were observed on chest CT, mainly under the pleura of lungs. Once DILD occurs, the suspected antineoplastic agents were stopped and glucocorticoid was given, among which 83.33% (10/12) patients were treated with antibiotics. Finally, 16.67% (2/12) were cured, 33.33% (4/12) were improved, 16.67% (2/12) were not cured and 33.33% (4/12) were dead.

Conclusion: Antineoplastic agents-induced ILD is mostly found in elderly male lung cancer patients with smoking history. The clinical symptoms of DILD are diverse and lack of specificity. ICIs-ILD has the characteristic of high incidence and poor prognosis compared with other antineoplastic agents. Comprehensive evaluation before medication, regular review, early and adequate glucocorticoid shock therapy after onset can improve the prognosis of DILD patients.
Evaluating the role of serum uric acid in the risk stratification and therapeutic response of patients with pulmonary arterial hypertension associated with congenital heart disease (PAH-CHD)
Authors: Luo Jun , Li Yuanchang , Chen Jingyuan , Qiu Haihua , Chen Wenjie , Luo Xiaoqin , Chen Yusi , Tan Yingjie , Li Jiang
Published: August 2023

Background: Pulmonary arterial hypertension (PAH) is a malignant pulmonary vascular disease that negatively impacts quality of life, exercise capacity, and mortality. This study sought to investigate the relationship between serum uric acid (UA) level and the disease severity and treatment response of patients with PAH and congenital heart disease (PAH-CHD).

Methods: This study included 225 CHD patients and 40 healthy subjects. Serum UA was measured in all patients, and UA levels and haemodynamic parameters were re-evaluated in 20 patients who had received PAH-specific drug treatment for at least 7 ± 1 month.

Results: Serum UA levels were significantly higher in PAH-CHD patients than in CHD patients with a normal pulmonary artery pressure and normal subjects (347.7 ± 105.7 μmol/L vs. 278.3 ± 84.6 μmol/L; 347.7 ± 105.7 μmol/L vs. 255.7 ± 44.5 μmol/L, p < 0.05). UA levels in the intermediate and high risk groups were significantly higher than those in the low-risk group (365.6 ± 107.8 μmol/L vs. 311.2 ± 82.8 μmol/L; 451.6 ± 117.6 μmol/L vs. 311.2 ± 82.8 μmol/L, p < 0.05). Serum UA levels positively correlated with mean pulmonary arterial pressure, WHO functional class, pulmonary vascular resistance, and NT-proBNP (r = 0.343, 0.357, 0.406, 0.398; p < 0.001), and negatively with mixed venous oxygen saturation (SvO2) and arterial oxygen saturation (SaO2) (r = −0.293, −0.329; p < 0.001). UA significantly decreased from 352.7 ± 97.5 to 294.4 ± 56.8 μmol/L (p = 0.001) after PAH-specific drug treatment for at least 6 months, along with significant decreases in mean pulmonary arterial pressure and pulmonary vascular resistance and increases in cardiac index and mixed SvO2.

Conclusion: Serum UA can be used as a practical and economic biomarker for risk stratification and the evaluation of PAH-specific drug treatment effects for patients with PAH-CHD.
Morphological, anatomical and histological studies on knob and beak characters of six goose breeds from China
Authors: Zhang Yang , Xu Xinlei , Ji Wangyang , Qi Shangzong , Bao Qiang , Zhang Yong , Zhang Yu , Xu Qi , Chen Guohong
Published: August 2023

The knob serves as both a sexual indicator of a goose’s maturity and a significant packaging attribute that garners consumer attention. However, studies regarding the morphological, anatomical and histological traits of different breeds and ages on the on knob in goose are lacking. In this study, six breeds with typical goose knob types were selected, and their knob size, morphological, anatomical and histological traits were characterized. The results showed that: Knob was more prominent in gander than in female goose, and the difference was the most obvious in Magang goose. Wanxi white goose and Shitou goose had the largest knob bulge, while Magang goose and Sichuan white goose were smaller. The total knob volume of Wanxi White goose and Shitou goose was significantly higher than that of other breeds, regardless of male or female (p < 0.05). The beak volume of Wanxi White goose and gander was significantly higher than that of other goose breeds (p < 0.05). Furthermore, the observation revealed that the “knob” primarily consisted of skin-derived tissue and bony protrusions. As age advances, the knob of both male and female geese undergoes synchronous development, with the knob of male geese typically surpassing that of their female counterparts during the same period. The growth rate of knob in male goose was the fastest from 70 to 120 days of age, and slowed down from 300 to 500 days of age. The growth rate of knob in female goose was slower than that in male goose. There were essential differences in the composition of Yangzhou goose knob and Magang goose knob. The subcutaneous tissue of Magang goose was rich, and the thickness of epidermis, dermis and various layers was significantly smaller than that of Yangzhou goose (p < 0.05). With the growth of goose knob, the cells of the epidermal spinous layer became denser and gradually condensed into an overall structure, and there was a clear boundary between the dermis and epidermis after adult. In adulthood, the fiber fascicle network was staggered and dense, with greater toughness and elasticity, and the stratum corneum, epidermis, reticular layer, dermis and other skin structural layers became thicker.
Adenosine stimulates the basolateral 50 pS K+ channel in renal proximal tubule via adenosine-A1 receptor
Authors: Liu Hao , Sun Qi , Ding Zheng , Shi Wensen , Wang Wen-Hui , Zhang Chengbiao
Published: August 2023

Background: The basolateral potassium channels play an important role in maintaining the membrane transport in the renal proximal tubules (PT) and adenosine receptors have been shown to regulate the trans-epithelial Na+ absorption in the PT. The aim of the present study is to explore whether adenosine also regulates the basolateral K+ channel of the PT and to determine the adenosine receptor type and the signaling pathway which mediates the effect of adenosine on the K+ channel.

Methods: We have used the single channel recording to examine the basolateral K+ channel activity in the proximal tubules of the mouse kidney. All experiments were performed in cell-attached patches.

Results: Single channel recording has detected a 50 pS inwardly-rectifying K+ channel with high channel open probability and this 50 pS K+ channel is a predominant type K+ channel in the basolateral membrane of the mouse PT. Adding adenosine increased 50 pS K+ channel activity in cell-attached patches, defined by NPo (a product of channel Numbers and Open Probability). The adenosine-induced stimulation of the 50 pS K+ channel was absent in the PT pretreated with DPCPX, a selective inhibitor of adenosine A1 receptor. In contrast, adenosine was still able to stimulate the 50 pS K+ channel in the PT pretreated with CP-66713, a selective adenosine A2 receptor antagonist. This suggests that the stimulatory effect of adenosine on the 50 pS K+ channel of the PT was mediated by adenosine-A1 receptor. Moreover, the effect of adenosine on the 50 pS K+ channel was blocked in the PT pretreated with U-73122 or Calphostin C, suggesting that adenosine-induced stimulation of the 50 pS K+ channels of the PT was due to the activation of phospholipase C (PLC) and protein kinase C (PKC) pathway. In contrast, the inhibition of phospholipase A2 (PLA2) with AACOCF3 or inhibition of protein kinase A (PKA) with H8 failed to block the adenosine-induced stimulation of the 50 pS K+ channel of the PT.

Conclusion: We conclude that adenosine activates the 50 pS K+ channels in the basolateral membrane of PT via adenosine-A1 receptor. Furthermore, the effect of adenosine on the 50 pS K+ channel is mediated by PLC-PKC signaling pathway.
Transcriptome analysis of two tobacco varieties with contrast resistance to Meloidogyne incognita in response to PVY MSNR infection
Authors: Xu Shixiao , Tian Pei , Jiang Zhimin , Chen Xiaoxiang , Li Bo , Sun Jutao , Zhang Zhiqiang
Published: August 2023

Root-knot nematode (RKN) disease is a major disease of tobacco worldwide, which seriously hinders the improvement of tobacco yield and quality. Obvious veinal necrosis-hypersensitive responses are observed only in RKN-resistant lines infected by Potyvirus Y (PVY) MSNR, making this an effective approach to screen for RKN-resistant tobacco. RNA-seq analysis, real-time quantitative PCR (qRT-PCR) and functional enrichment analysis were conducted to gain insight into the transcription dynamics difference between G28 (RKN-resistant) and CBH (RKN-susceptible) varieties infected with PVY MSNR. Results showed that a total of 7900, 10576, 9921, 11530 and 12531 differentially expressed genes (DEGs) were identified between the two varieties at 0, 1, 3, 5, and 7 d after infection, respectively. DEGs were associated with plant hormone signal transduction, starch and sucrose metabolism, phenylpropanoid biosynthesis, and photosynthesis-related metabolic pathways. Additional DEGs related to starch and sucrose metabolism, energy production, and the indole-3-acetic acid signaling pathway were induced in CBH plants after infection. DEGs related to phenylpropanoid biosynthesis, abscisic acid, salicylic acid, brassinosteroids, and jasmonic acid signaling pathway were induced in G28 after infection. Our findings reveal DEGs that may contribute to differences in PVY MSNR resistance among tobacco varieties. These results help us to understand the differences in transcriptional dynamics and metabolic processes between RKN-resistant and RKN-susceptible varieties involved in tobacco-PVY MSNR interaction.
CRISPR/Cas9-mediated editing of PHYTOENE DESATURASE gene in onion (Allium cepa L.)
Authors: Mainkar Pawan , Manape Tushar Kashinath , Satheesh Viswanathan , Anandhan Sivalingam
Published: August 2023

Introduction

Clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated protein 9 (Cas9) is a precise genome editing tool used to introduce genetic modifications in a wide range of crop species. Thus far, there is no report of CRISPR/Cas9-mediated genome editing in onions (Allium cepa L.).

Methods

In the present study, we targeted two exons of the gene coding for Phytoene desaturase (AcPDS) in onion cv. Bhima Super. The sgRNA-carrying constructs were co-cultivated with 8-week-old embryogenic calli using an Agrobacterium-mediated transformation protocol and incubated on the media without hygromycin B selection.

Results and discussion

Out of the total 617 co-cultivated calli, 21 (3.4%) regenerated shoots exhibited three distinct phenotypes: albino, chimeric, and pale green; in comparison to the wild-type non-transformed regenerated shoots. Total chlorophyll content was drastically reduced in albino shoots and significantly decreased in chimeric shoots. Out of the six Cas9 gene PCR-confirmed regenerated shoots, two exhibited the albino phenotype due to insertions/deletions (InDels) and substitution-based mutations in and around the AcPDS target sites. Deep amplicon sequencing revealed a significantly variable InDel frequency between two sgRNAs, ranging from 1.2% to 63.4%, along with a 53.4% substitution frequency. The mutation of the AcPDS gene generated a visually detectable albino phenotype, thus confirming the successful editing of the AcPDS gene. This is the first time a CRISPR/Cas9-mediated genome editing protocol has been successfully established in onion, with the AcPDS gene serving as an example. This study will provide the necessary momentum for researchers to further basic and applied research on onions.
Investigating the genetic basis of maize ear characteristics: a comprehensive genome-wide study utilizing high-throughput phenotypic measurement method and system
Authors: Wang Jinglu , Zhao Shuaihao , Zhang Ying , Lu Xianju , Du Jianjun , Wang Chuanyu , Wen Weiliang , Guo Xinyu , Zhao Chunjiang
Published: August 2023

The morphology of maize ears plays a critical role in the breeding of new varieties and increasing yield. However, the study of traditional ear-related traits alone can no longer meet the requirements of breeding. In this study, 20 ear-related traits, including size, shape, number, and color, were obtained in 407 maize inbred lines at two sites using a high-throughput phenotypic measurement method and system. Significant correlations were found among these traits, particularly the novel trait ear shape (ES), which was correlated with traditional traits: kernel number per row and kernel number per ear. Pairwise comparison tests revealed that the inbred lines of tropical-subtropical were significantly different from other subpopulations in row numbers per ear, kernel numbers per ear, and ear color. A genome-wide association study identified 275, 434, and 362 Single nucleotide polymorphisms (SNPs) for Beijing, Sanya, and best linear unbiased prediction scenarios, respectively, explaining 3.78% to 24.17% of the phenotypic variance. Furthermore, 58 candidate genes with detailed functional descriptions common to more than two scenarios were discovered, with 40 genes being associated with color traits on chromosome 1. After analysis of haplotypes, gene expression, and annotated information, several candidate genes with high reliability were identified, including Zm00001d051328 for ear perimeter and width, zma-MIR159f for ear shape, Zm00001d053080 for kernel width and row number per ear, and Zm00001d048373 for the blue color channel of maize kernels in the red-green-blue color model. This study emphasizes the importance of researching novel phenotypic traits in maize by utilizing high-throughput phenotypic measurements. The identified genetic loci enrich the existing genetic studies related to maize ears.
External and internal influences yield similar memory effects: the role of deception and suggestion
Authors: Otgaar Henry , Mangiulli Ivan , Battista Fabiana , Howe Mark L.
Published: August 2023

In legal cases, testimonies can become contaminated because of an amalgam of external and internal influences on memory. It is well-established that external influences (e.g., suggestive interviews) can hurt memory. However, less focus has been placed on the impact of internal influences (e.g., lying) on memory. In the current review, we show that the available evidence suggests that both external and internal influences exert similar effects on memory. That is, we review studies showing that suggesting non-occurrences and suggesting non-experiences can lead to omission errors and false memories, respectively. Likewise, these memory effects are also observed when focusing on internal influences. That is, false denials, feigning amnesia and fabrication have been shown to affect memory in terms of forgetting (i.e., omissions) and false memories (i.e., commissions). Also, we show that both external and internal influences can lead to changes in the belief that an event occurred. We argue that in legal cases, triers of fact should concentrate on whether both types of influences might have affected testimonial accuracy in witnesses, victims, and suspects.
“Asking for help is a strength”—how to promote undergraduate medical students’ teamwork through simulation training and interprofessional faculty
Authors: Kolbe Michaela , Goldhahn Jörg , Useini Mirdita , Grande Bastian
Published: August 2023

The ability to team up and safely work in any kind of healthcare team is a critical asset and should be taught early on in medical education. Medical students should be given the chance to “walk the talk” of teamwork by training and reflecting in teams. Our goal was to design, implement and evaluate the feasibility of a simulation-based teamwork training (TeamSIM) for undergraduate medical students that puts generic teamwork skills centerstage. We designed TeamSIM to include 12 learning objectives. For this pre-post, mixed-methods feasibility study, third-year medical students, organized in teams of 11–12 students, participated and observed each other in eight simulations of different clinical situation with varying degrees of complexity (e.g., deteriorating patient in ward; trauma; resuscitation). Guided by an interprofessional clinical faculty with simulation-based instructor training, student teams reflected on their shared experience in structured team debriefings. Using published instruments, we measured (a) students’ reactions to TeamSIM and their perceptions of psychological safety via self-report, (b) their ongoing reflections via experience sampling, and (c) their teamwork skills via behavior observation. Ninety four students participated. They reported positive reactions to TeamSIM (M = 5.23, SD = 0.5). Their mean initial reported level of psychological safety was M = 3.8 (SD = 0.4) which rose to M = 4.3 (SD = 0.5) toward the end of the course [T(21) = −2.8, 95% CI −0.78 to-0.12, p = 0.011 (two-tailed)]. We obtained n = 314 headline reflections from the students and n = 95 from the faculty. For the students, the most frequent theme assigned to their headlines involved the concepts taught in the course such as “10 s for 10 min.” For the faculty, the most frequent theme assigned to their headlines were reflections on how their simulation session worked for the students. The faculty rated students’ teamwork skills higher after the last compared to the first debriefing. Undergraduate medical students can learn crucial teamwork skills in simulations supported by an experienced faculty and with a high degree of psychological safety. Both students and faculty appreciate the learning possibilities of simulation. At the same time, this learning can be challenging, intense and overwhelming. It takes a team to teach teamwork.
Depression and anxiety in acute ischemic stroke involving the anterior but not paramedian or inferolateral thalamus
Authors: Scharf Anne-Carina , Gronewold Janine , Eilers Andres , Todica Olga , Moenninghoff Christoph , Doeppner Thorsten R. , de Haan Bianca , Bassetti Claudio L. , Hermann Dirk M.
Published: August 2023

Background and objectives

Emotional and cognitive deficits are prevalent in strokes involving the thalamus. In contrast to cognitive deficits, emotional deficits have not been studied prospectively in isolated thalamic stroke.

Methods

In 37 ischemic thalamic stroke patients (57.0 [50.0; 69.5] years [median (Q1; Q3)], 21 males, 5 anterior, 12 paramedian, 20 inferolateral vascular territory), and 37 non-stroke control patients matched for age and sex, we prospectively examined depression, anxiety, activities of daily living, and quality of life at 1, 6, 12, and 24 months post-stroke using the Hospital-Anxiety-and-Depression Scale (HADS), Nürnberger-Alters-Alltagsaktivitäten scale (NAA), and Short Form-36 (SF36) questionnaire. Voxel-based lesion-symptom mapping (VLSM) and lesion-subtraction analyzes were performed to determine associations between questionnaire scores and thalamic stroke topography.

Results

At 1 month post-stroke, anterior thalamic stroke patients had higher depression scores [8.0 (7.5; 10.5)] than paramedian [4.5 (1.0; 5.8)] and inferolateral [4.0 (1.0; 7.0)] thalamic stroke patients. Furthermore, anterior thalamic stroke patients had higher anxiety scores [11.0 (8.0; 14.5)] than their matched controls [2.5 (2.0; 2.5)], paramedian [4.5 (1.0; 5.8)] and inferior [4.0 (1.0; 7.0)] thalamic stroke patients. Depression and anxiety scores in anterior thalamic stroke patients remained high across the follow-up [depression: 9.0 (3.5; 13,8); anxiety:10.05 (2.8, 14.5)].

Physical health assessed by SF36 was intact in anterior [1 month post-stroke: T-score = 55.9 (37.0; 57.6)] but reduced in inferolateral [44.5(32.4; 53.1)] thalamic stroke, whereas mental health was reduced in anterior thalamic stroke [32.0 (29.8; 47.3)].

VLSM confirmed that voxels in the anterior thalamus around Montreal Neurological Institute (MNI) coordinates X = −8, Y = −12, Z = 2 were more often affected by the stroke in depressed (HADS-score ≥ 8) than non-depressed (HADS-score < 8) patients and voxels around coordinates X = −10, Y = −12, Z = 2 were more often affected in anxious (HADS-score ≥ 8) than non-anxious (HADS-score < 8) patients.

Conclusion

Anterior, but not paramedian or inferolateral thalamic stroke was associated with depression and anxiety. Even though our results are mostly significant in the left thalamus, this observation on stroke laterality might be confounded by the fact that the right hemisphere was underrepresented in our study.
Factors affecting resilience in Namibian children exposed to parental divorce: a Q-Methodology study
Authors: Van Schalkwyk Janine , Gentz Shelene
Published: August 2023

Introduction

Divorce is a contributor to family instability within sub-Saharan Africa, and specifically within Namibia, an increasing number of children are exposed to its impact. However, not all children react uniformly to the impact of parental divorce, and many children may be resilient. Understanding what promotes resilience in children post-divorce in African contexts is vital, given the unique socio-cultural context. Therefore, this study aimed to understand how some children are capable of resilience despite exposure to parental divorce in Namibia.

Methods

A multiple case study design was employed to assess the lived experiences of children aged 9–12 post-parental divorce in Windhoek. Using the Child and Youth Resilience Measurement (CYRM-12) scale, 24 children exposed to parental divorce were screened for resiliency. The Q-Methodology, with visual material, was utilized with a sub-sample of 12 children who scored high on the CYRM (50% girls, mean age = 11) to eliminate some of the challenges associated with gathering qualitative data from younger children. The PQ Method 2.35 software program was used for data analysis.

Results

By-person factor analysis identified four statistically significant profiles. A third (33%) of participants loaded on a factor emphasizing “quality parent-child relationships” and a further 33% emphasizing “effective parent conflict resolution.” The final two factors emphasized “healthy school attachment” (17%) and “strong community attachment” (17%). All children emphasized a stable, loving familial environment, and frequent visitation with the non-custodial father.

Discussion

Our findings suggest that multiple social ecologies nurture resilience in children exposed to parental divorce in Namibia. Support should be extended beyond the perimeters of the nuclear family, and relationships with extended family members, peer groups, school, and the wider community can play an important role in children's adjustment. The study highlights the importance of contextually grounded resilience as some factors that are emphasized for children from more Western communities do not reflect as strongly in the results of this study. Other factors, including a stronger reliance on community and factors such as the school, peers, and extended family members, may play a bigger role in child resilience post-divorce in Namibia.
Adverse childhood experiences in patients with schizophrenia: related factors and clinical implications
Authors: Zhang Lei , Zhao Nan , Zhu Minghuan , Tang Minyi , Liu Weiqing , Hong Wenjuan
Published: August 2023

The relationship between adverse childhood experiences (ACEs) and the development of psychotic symptoms is not well understood. Therefore, this study aimed to investigate the frequency and distribution of ACEs among patients with schizophrenia and their potential correlation with symptomatology and personality pathology. We conducted a cross-sectional study involving 571 patients with schizophrenia in Shanghai, China. Symptomatology was assessed using the Positive and Negative Symptoms Scale (PANSS) and personality pathology was assessed using the Personality Diagnostic Questionnaire Fourth Edition Plus (PDQ-4+). ACEs were assessed using the Child Trauma Questionnaire-Short Form (CTQ-SF). ACEs were highly prevalent, with 80.8% of the patients with schizophrenia reporting at least one ACE. The three most common types of ACE were physical neglect (69.8%), emotional neglect (28.2%), and emotional abuse (22.9%). For specific ACE, emotional abuse was significantly associated with PD traits, whereas emotional and physical neglect types of ACE was significantly associated with negative symptoms. A higher level of physical abuse was more commonly reported by men, younger individuals, and those with a higher level of antisocial PD traits. Higher levels of physical neglect were associated with more severe negative symptoms. ACEs are commonly observed in patients with schizophrenia. Therefore, it is strongly recommended that this clinical population be provided with a comprehensive assessment and individualized intervention for those exposed to specific ACEs.
Investigating women’s awareness and perceptions on human papillomavirus infection and oropharyngeal cancer in Italy
Authors: Paduano Giovanna , Vaienna Sara , Maisto Giuseppe , Di Giuseppe Gabriella , Pavia Maria
Published: August 2023

Introduction

This study explored knowledge, attitudes and behaviors toward human papillomavirus (HPV) infection and oropharyngeal cancer (OPC) among women attending primary care services.

Methods

The cross-sectional study was conducted from September to December 2022 in adult women attending three primary care services in Italy, who were invited to complete a self-administered questionnaire.

Results

Overall, 34.7% of participants know that OPC is HPV-associated; knowledge was higher among women who had personal, familiar or friends’ experience of cancer, and who reported to often seek dental care, whereas it was lower in older women. The perception of risk of developing OPC was high for 26.4% of women and was higher in those who had experienced STD, who knew that early sexual debut is a risk factor for OPC, and who considered useful the role of dentists on the provision of information about OPC. Only 22.5% had received HPV vaccination, but 62% intended to receive it in the future. Moreover, 63.2% believe that HPV vaccination is very useful to prevent OPC and only 27% are concerned about health consequences of HPV vaccination.

Conclusion

These findings indicate that women’s awareness of the role of HPV infection in the development of OPC is not satisfactory and underline the role that dentists might have in improving their patients’ awareness on HPV related OPC.
HPV prevalence and genotype distribution in 2,306 patients with cervical squamous cell carcinoma in central and eastern China
Authors: Han Chunrong , Huang Wanqiu , Ye Mei , Zou Rong , Lan Jianyun , Chen Jing , Jiang Jingui , Wang Hongjing , Xia Lin , Luo Jun , Li Dongbin , Geng Jianxiang , Wang Zhihui , Huang Jian
Published: August 2023

Background

To explore the positivity rate and genotype distribution of human papillomavirus (HPV) in cervical squamous cell carcinoma (CSCC) tissues in central and eastern China and to provide theoretical basis for cervical cancer screening and prophylactic HPV vaccine development in China.

Methods

DNA was extracted from paraffin-embedded tissues of CSCC samples and exfoliated cervical cells of cervical cancer screening populations. 23 HPV genotypes were detected by combining polymerase chain reaction (PCR) and reverse dot hybridized gene chip detection technology in 2,306 CSCC tissues and 10,245 cervical cancer screening populations. The genotype distribution of HPV infection was analyzed.

Results

The overall infection rate of HPVs in 2,306 CSCC patients was 92.71%. The frequency of single-type HPV infection and multiple-type HPV infection were 86.48% and 13.51%, respectively. The most common HPV genotypes detected in Chinese CSCC tissues were HPV-16, HPV-18, HPV-31, HPV-33, HPV-45, HPV-52, HPV-58, and HPV-59. The overall positivity rate of these eight high-risk HPV (HR-HPV) genotypes in HPV-positive CSCC was as high as 96.91%. Of which the positivity rate of seven HR-HPV genotypes related to nine-valent HPV vaccines in HPV-positive CSCC was 95.09%. Meanwhile, the overall infection rates of HR-HPV and low-risk HPV (LR-HPV) in female aged 35–64 years who underwent cervical cancer screening were 13.16% and 1.32%, respectively. The high-frequency HR-HPV genotypes in cervical cancer screening women were HPV-52, HPV-58, HPV-16, HPV-53, HPV-68, HPV-39, HPV-51, and HPV-56, with positivity rates of 2.25%, 1.60%, 1.31%, 1.22%, 0.93%, 0.92%, 0.78%, and 0.74%, respectively.

Conclusion

Among women screened for cervical cancer in China, detecting the 8 high-frequency HR-HPV genotypes can reduce technical difficulty and reagent costs, while also improving the efficiency and effectiveness of cervical cancer screening. HPV genotyping assists gynecologists in assessing the risk of HR-HPV-positive cervical intraepithelial neoplasia and guiding them in implementing appropriate interventions. Furthermore, HPV genotyping is helpful for doctors to follow up HR-HPV-positive women and to evaluate the protective effect of HPV vaccine.
Cloudy or sunny? Effects of different environmental types of urban green spaces on public physiological and psychological health under two weather conditions
Authors: Cao Saixin , Shang Zike , Li Xi , Luo Hao , Sun Lingxia , Jiang Mingyan , Du Juan , Fu Erkang , Ma Jun , Li Nian , Guo Baimeng , Yu Xiaofang , Lv Bingyang , Wang Jinde
Published: August 2023

Numerous studies have demonstrated that urban green spaces (UGSs) benefit human health, but few have focused on the influence of weather on environmental restorativeness. This study assessed how different weather conditions and environments affect human health. We exposed 50 participants to different UGS environments under cloudy and sunny conditions and collected physiological, psychological and aesthetic preference data. The result showed that the physical and mental benefits of UGSs were stronger on sunny days (pulse: [t = 2.169, p < 0.05]; positive affect: [Z = −10.299, p < 0.001]; perceived restortiveness: [Z = −3.224, p < 0.01]). The spaces with exposed sky had greater physiological restorativeness on sunny days; the spaces with calm water had greater emotional restorativeness on cloudy days, and natural spaces with less sky exposure had greater perceived restoration in both weather conditions. The spaces with water and less sky exposure promoted psychophysiological restoration in both weather conditions. This study demonstrates that weather significantly influences the restorative potential of UGSs, and there are also restorative variations in different green space environments under two weather conditions. In future UGS planning practices, it would be advisable to select appropriate environmental types and features based on the climatic characteristics of different regions. For instance, in areas with frequent overcast conditions, incorporating serene water bodies could be advantageous, while regions with predominantly sunny weather should encompass spaces with expansive sky views. By conducting comprehensive research on restoration environments that take weather conditions into account, new insights and nature-based solutions can be provided for creating healthy human habitats in the context of global climate change.
In bot we trust? Personality traits and reciprocity in human-bot trust games
Authors: Upadhyaya Nitish , Galizzi Matteo M.
Published: August 2023

People are increasingly interacting with forms of artificial intelligence (AI). It is crucial to understand whether accepted evidence for human-human reciprocity holds true for human-bot interactions. In a pre-registered online experiment (N = 539) we first replicate recent studies, finding that the identity of a player's counterpart in a one-shot binary Trust Game has a significant effect on the rate of reciprocity, with bot counterparts receiving lower levels of returned amounts than human counterparts. We then explore whether individual differences in a player's personality traits—in particular Agreeableness, Extraversion, Honesty-Humility and Openness—moderate the effect of the identity of the player's counterpart on the rate of reciprocity. In line with literature on human-human interactions, participants exhibiting higher levels of Honesty-Humility, and to a lesser extent Agreeableness, are found to reciprocate more, regardless of the identity of their counterpart. No personality trait, however, moderates the effect of interacting with a bot. Finally, we consider whether general attitudes to AI affect the reciprocity but find no significant relationship.
Preferences for the shared use of electric-powered vehicles in mobility packages: an empirical analysis of MaaS in a university context
Authors: Decker Lea , Marx Christian P. , Weiβ Matthias , Werbeck Nicola
Published: August 2023

Previous research on preferences of potential Mobility-as-a-Service (MaaS) users often included public transport (PT) in bundled mobility options. Due to the often reported enormous importance of PT availability, including PT in the bundles may have led to distortions in the identification of other vehicle preferences in MaaS offerings. Our analysis counteracts this distortion and investigates how other means of transport are evaluated in mobility packages when PT is not part of the bundle. We included electric-powered mobility alternatives, which have rarely been considered in previous research, to find out which alternatives to PT users would prefer that might increase their approval of MaaS. Furthermore, the results of the existing literature are inconsistent in emphasizing whether sustainability and environmental protection are actually relevant in the valuation of different mobility options. Our analysis thus aims at identifying which electric vehicles are preferred in a mobility package, when PT is not included. We further investigate whether sustainability components of a mobility package influence the preference, structure of individuals. We based the empirical evaluation on a rank-ordered conjoint analysis, in which mobility packages were evaluated according to individual user preferences, including emissions savings. We therefore conducted a study to identify vehicle preferences other than PT and the valuation of sustainability aspects in mobility patterns. We surveyed 995 students to identify preferences and willingness to pay (WTP) for MaaS among this specific user group. Results of the empirical analysis show that preferences for an e-car and an e-scooter exist. Furthermore, we found that sustainability plays a role in the evaluation of mobility packages. Accordingly, we found, that a potential reduction in emissions leads to a higher approval of MaaS. Thus, the study extends the theoretical and empirical understanding of preference-ordering of MaaS packages and clarifies the importance of sustainability in transport choices.
Conflict and crime. Restorative justice in Italy
Authors: Palermo Giovanna
Published: August 2023

The article presents a socio-legal analysis of the Legislative Decree (Lgs.D.) of 10 October 2022, n.150, implementing the law of 27 September 2021, n.134 (so-called “Cartabia reform”), which provides for the introduction in Italy of a comprehensive framework regulation of restorative justice. The central theme of this paper is precisely the conflict connected to a crime, the offender-victim relationship and restorative justice in the light of the aforementioned decree. As Luhmann observes, the omnipresence of conflicts in society is self-evident. Even the crime produces a conflict or, at times, is the extreme expression of a pre-existing and sometimes latent conflict. The problem that arises is, therefore, that of the control and management of conflicts. These are usually managed by the law, but, given its limitations, it is increasingly necessary to encourage the use of restorative justice programs. On an interpersonal level, conflict triggers a negative mechanism of hostility and disavowal of the other, which either wraps itself in an explosive vortex, or deviates, becoming the pretext for abandoning the only form of communication that conflict makes possible, as Luhmann observes, the one based on “no”. The Italian regulatory intervention certainly appears relevant, albeit with some critical issues, and to be kept under observation for future application developments.
The notorious daredevils: potential toxic levels of cyanide and heavy metals in cassava flour sold in selected markets—taken Oke Ogun Community, Oyo State as an example
Authors: Jacob Omotoso Ayodele , Anuoluwa Omotoso Elizabeth , Raimi Morufu Olalekan
Published: August 2023

Pollution spread throughout terrestrial and aquatic ecosystems, and many remain transported around the globe by air transport. Furthermore, food as well as production chains remain dispersed through the global economy. Thus, the current study examined the potentially harmful amounts of cyanide as well as trace metals in cassava flour sold in Oke Ogun community township markets. Its goal was to quantify the degrees of Lead, Cyanide, Arsenic and Chromium, evaluate their health impacts on customers, and evaluate WHO-allowable dietary quantities. Using a stratified sampling strategy, five township markets were visited to obtain samples of finely ground fermented cassava flour (Kishi, Igbeti, Igboho, Iseyin, and Shaki). The materials were properly digested before being examined with an Atomic Absorption Spectrophotometer (AAS). Lead (0.028–0.053 mg/L), Cyanides (0.010–0.018 mg/L), Chromium (0.034–0.065 mg/L) and Arsenic (0.006–0.012 mg/L), were the results obtained. At conclusion, due to the lower content of these metals, the cassava flour sold in Oke Ogun community markets is safe as well as appropriate for human utilization, with no nutritional risk consequences. As a result, it is advised that cassava flour marketed be closely checked and assessed on a continuous basis. Therefore, regular monitoring of toxic metals in Nigeria is strongly advised in order to avoid a significant environmental and public health issue.
Neural-Network-Assisted Finite Difference Discretization for Numerical Solution of Partial Differential Equations
Authors: Izsák Ferenc , Izsák Rudolf
Published: August 2023

A neural-network-assisted numerical method is proposed for the solution of Laplace and Poisson problems. Finite differences are applied to approximate the spatial Laplacian operator on nonuniform grids. For this, a neural network is trained to compute the corresponding coefficients for general quadrilateral meshes. Depending on the position of a given grid point x0 and its neighbors, we face with a nonlinear optimization problem to obtain the finite difference coefficients in x0. This computing step is executed with an artificial neural network. In this way, for any geometric setup of the neighboring grid points, we immediately obtain the corresponding coefficients. The construction of an appropriate training data set is also discussed, which is based on the solution of overdetermined linear systems. The method was experimentally validated on a number of numerical tests. As expected, it delivers a fast and reliable algorithm for solving Poisson problems.