bluuebunny/crossref_metadata_2025 huggingface dataset:
Long Term Follow-Up on Pediatric Cases With Congenital Myasthenic Syndromes—A Retrospective Single Centre Cohort Study
Authors: Della Marina Adela , Wibbeler Eva , Abicht Angela , Kölbel Heike , Lochmüller Hanns , Roos Andreas , Schara Ulrike
Published: December 2020

Introduction: Congenital myasthenic syndromes (CMS) refer to a heterogenic group of neuromuscular transmission disorders. CMS-subtypes are diverse regarding exercise intolerance and muscular weakness, varying from mild symptoms to life-limiting forms with neonatal onset. Long-term follow-up studies on disease progression and treatment-response in pediatric patients are rare.

Patients and Methods: We analyzed retrospective clinical and medication data in a cohort of 32 CMS-patients including the application of a standardized, not yet validated test (CMS-ST) to examine muscular strength and endurance in 21 patients at the last follow-up. Findings obtained in our cohort were compared with long-term follow-up studies of (adult) CMS-cohorts from the literature by considering the underlying molecular mechanisms. Outcomes of CMS-ST were compared to results of normal clinical assessment.

Results: Thirty-two pediatric patients with defects in eight different CMS-genes were followed by a median time of 12.8 years. Fifty-nine percentage of patients manifested with first symptoms as neonates, 35% as infants. While 53% of patients presented a reduced walking distance, 34% were wheelchair-bound. Even under adequate therapy with pyridostigmine (PS) and 3,4-diaminopyridine, CHAT-mutations led to the progression of muscular weakness partly in combination with persistent respiratory and bulbar symptoms. RAPSN, CHRND, and CHRNB1 patients with neonatal manifestation, early respiratory problems, and bulbar symptoms showed a good and maintained treatment response. CHAT and CHRNE patients required higher PS dosages, whereas RAPSN patients needed a lower mean dosage at the last follow-up. The benefits of short-term medication and long-term progression of symptoms were highly dependent on the specific genetic defect. CMS-ST was carried out in 17/21 patients, determined affected muscle groups including bulbar and ocular symptoms, some of which were not reported by the patients.

Conclusions: Our findings and comparison with the literature- suggest a better treatment-response and less severe progression of symptoms present in patients suffering from mutations in CMS-genes directly associated with receptor deficiency, while patients with defects leading to synaptopathy and presynaptic defects tend to have worse outcomes. Assessment of affected muscular groups and clinical symptoms by CMS-ST may be a useful tool for optimal therapeutic management of the patients, especially for future clinical studies.
Quantitative Multi-Parameter Mapping Optimized for the Clinical Routine
Authors: Cooper Graham , Hirsch Sebastian , Scheel Michael , Brandt Alexander U. , Paul Friedemann , Finke Carsten , Boehm-Sturm Philipp , Hetzer Stefan
Published: December 2020

Using quantitative multi-parameter mapping (MPM), studies can investigate clinically relevant microstructural changes with high reliability over time and across subjects and sites. However, long acquisition times (20 min for the standard 1-mm isotropic protocol) limit its translational potential. This study aimed to evaluate the sensitivity gain of a fast 1.6-mm isotropic MPM protocol including post-processing optimized for longitudinal clinical studies. 6 healthy volunteers (35±7 years old; 3 female) were scanned at 3T to acquire the following whole-brain MPM maps with 1.6 mm isotropic resolution: proton density (PD), magnetization transfer saturation (MT), longitudinal relaxation rate (R1), and transverse relaxation rate (R2*). MPM maps were generated using two RF transmit field (B1+) correction methods: (1) using an acquired B1+ map and (2) using a data-driven approach. Maps were generated with and without Gibb's ringing correction. The intra-/inter-subject coefficient of variation (CoV) of all maps in the gray and white matter, as well as in all anatomical regions of a fine-grained brain atlas, were compared between the different post-processing methods using Student's t-test. The intra-subject stability of the 1.6-mm MPM protocol is 2–3 times higher than for the standard 1-mm sequence and can be achieved in less than half the scan duration. Intra-subject variability for all four maps in white matter ranged from 1.2–5.3% and in gray matter from 1.8 to 9.2%. Bias-field correction using an acquired B1+ map significantly improved intra-subject variability of PD and R1 in the gray (42%) and white matter (54%) and correcting the raw images for the effect of Gibb's ringing further improved intra-subject variability in all maps in the gray (11%) and white matter (10%). Combining Gibb's ringing correction and bias field correction using acquired B1+ maps provides excellent stability of the 7-min MPM sequence with 1.6 mm resolution suitable for the clinical routine.
The Serpentine Illusion: A Visual Motion Illusion Induced by Phase-Shifted Line Gratings
Authors: Luo Junxiang , Chen Zheyuan , Lu Yiliang , Spillmann Lothar , Andolina Ian Max , Wang Wei
Published: December 2020

In a pattern of horizontal lines containing ± 45° zigzagging phase-shifted strips, vivid illusory motion is perceived when the pattern is translated up or down at a moderate speed. Two forms of illusory motion are seen: [i] a motion “racing” along the diagonal interface between the strips and [ii] lateral (sideways) motion of the strip sections. We found the relative salience of these two illusory motions to be strongly influenced by the vertical spacing and length of the line gratings, and the period length of the zigzag strips. Both illusory motions are abolished when the abutting strips are interleaved, separated by a gap or when a real line is superimposed at the interface. Illusory motion is also severely weakened when equiluminant colored grating lines are used. Illusory motion perception is fully restored at < 20% luminance contrast. Using adaptation, we find that line-ends alone are insufficient for illusory motion perception, and that both physical carrier motion and line orientation are required. We finally test a classical spatiotemporal energy model of V1 cells that exhibit direction tuning changes that are consistent with the direction of illusory motion. Taking this data together, we constructed a new visual illusion and surmise its origin to interactions of spatial and temporal energy of the lines and line-ends preferentially driving the magnocellular pathway.
Is a Higher Protein-Lower Glycemic Index Diet More Nutritious Than a Conventional Diet? A PREVIEW Sub-study
Authors: Meroni Alice , Muirhead Roslyn P. , Atkinson Fiona S. , Fogelholm Mikael , Raben Anne , Brand-Miller Jennie C.
Published: December 2020

High protein diets and low glycemic index (GI) diets have been associated with improved diet quality. We compared the changes in nutrient intakes of individuals at high risk of developing type-2 diabetes over 3 y who followed either a higher protein-lower GI diet (HPLG) or a conventional moderate protein-moderate GI diet (MPMG). This post hoc analysis included 161 participants with overweight and pre-diabetes from the Australian cohort of the PREVIEW study (clinical trial registered in https://www.clinicaltrials.gov/ct2/show/NCT01777893?term=NCT01777893&draw=2&rank=1) who were randomly assigned to a HPLG diet (25% energy from protein, dietary GI ≤ 50, n = 85) or a MPMG diet (15% energy from protein, dietary GI ≥ 56, n = 76). Food records were collected at 0-mo (baseline) and at 6-, 12-, 24-, and 36-mo (dietary intervention period). Linear mixed models were used to compare the differences in total energy, macro- and micronutrients, dietary GI, glycemic load (GL) and body weight between the two diet groups at the 4 dietary intervention time points. At 3 y, 74% participants from the HPLG diet and 74% participants from the MPMG diet completed the trial. The HPLG group showed significantly higher protein intake and lower dietary GI and GL than the MPMG group (group fixed effect P < 0.001 for all three parameters). By 6-, 12-, 24-, and 36-mo there was a 3.0, 2.7, 2.2, and 1.4% point difference in protein intake and 6.2, 4.1, 4.8, and 3.9 GI unit difference between the groups. The intake of energy and saturated fat decreased (mostly in the first 6-mo), while the intake of dietary fiber increased (from mo-0 to mo-12 only) in both diets, with no significant differences between the diets. The dietary intakes of zinc (group fixed effect P = 0.05), selenium (P = 0.01), niacin (P = 0.01), vitamin B12 (P = 0.01) and dietary cholesterol (group by time fixed effect P = 0.001) were higher in the HPLG group than in the MPMG group. Despite both diets being designed to be nutritionally complete, a HPLG diet was found to be more nutritious in relation to some micronutrients, but not cholesterol, than a MPMG diet.
Zinc, Vitamin D and Vitamin C: Perspectives for COVID-19 With a Focus on Physical Tissue Barrier Integrity
Authors: Name José João , Souza Ana Carolina Remondi , Vasconcelos Andrea Rodrigues , Prado Pietra Sacramento , Pereira Carolina Parga Martins
Published: December 2020

Some nutrients play key roles in maintaining the integrity and function of the immune system, presenting synergistic actions in steps determinant for the immune response. Among these elements, zinc and vitamins C and D stand out for having immunomodulatory functions and for playing roles in preserving physical tissue barriers. Considering the COVID-19 pandemic, nutrients that can optimize the immune system to prevent or lower the risk of severe progression and prognosis of this viral infection become relevant. Thus, the present review aims to provide a comprehensive overview of the roles of zinc and vitamins C and D in the immune response to viral infections, focusing on the synergistic action of these nutrients in the maintenance of physical tissue barriers, such as the skin and mucous membranes. The evidence found in the literature shows that deficiency of one or more of these three elements compromises the immune response, making an individual more vulnerable to viral infections and to a worse disease prognosis. Thus, during the COVID-19 pandemic, the adequate intake of zinc and vitamins C and D may represent a promising pharmacological tool due to the high demand for these nutrients in the case of contact with the virus and onset of the inflammatory process. Ongoing clinical trials will help to clarify the role of these nutrients for COVID-19 management.
Immune Thrombocytopenia Induced by Immune Checkpoint Inhibitors in Solid Cancer: Case Report and Literature Review
Authors: Liu Xiaolin , Liang Xiuju , Liang Jing , Li Yan , Wang Jun
Published: December 2020

Immune checkpoint inhibitors, including antibodies targeting programmed cell death protein-1 (PD-1) and its receptor programmed cell death ligand-1 (PD-L1), represent promising therapeutic strategies for advanced human malignancies. However, a subgroup of patients experiences various autoimmune toxicities, termed immune-related adverse events (irAEs), that occur as a result of on-target and off-tumor autoimmune responses. Although irAEs are generally confirmed to be less severe than toxicities caused by conventional chemotherapy and targeted therapy, uncommon irAEs, such as immune thrombocytopenia, may occur with a very low incidence and sometimes be severe or fatal. This review focuses on the epidemiology, clinical presentation, and prognosis of immune thrombocytopenia occurring in advanced cancer patients induced by immune checkpoint inhibitors, especially in those with PD-1 or PD-L1 inhibitor treatment. We also first present one patient with non-small cell lung cancer who received the PD-L1 inhibitor durvalumab and developed severe thrombocytopenia.
Indoleamine 2, 3-Dioxygenase 1 and CD8 Expression Profiling Revealed an Immunological Subtype of Colon Cancer With a Poor Prognosis
Authors: Zhang Rixin , Li Tiegang , Wang Weiqi , Gan Wenqiang , Lv Silin , Zeng Zifan , Hou Yufang , Yan Zheng , Yang Min
Published: December 2020

Background

The Immunoscore method, based on the distribution of the quantification of cytotoxic and memory T cells, provides an indicator of tumor recurrence for colon cancer. However, recent evidence has suggested that immune checkpoint expression represents a surrogate measure of tumor-infiltrating T cell exhaustion, and therefore may serve as a more accurate prognostic biomarker for colon cancer. Indoleamine 2, 3-dioxygenase 1 (IDO1), a potent immunosuppressive molecule, has been strongly associated with T-cell infiltration, but it lacks universal prognostic significance among all of the cancer subtypes. Our aim was to elucidate the prognostic significance of the combination of IDO1 and CD8A expression in colon cancer.

Methods

Gene expression and clinical survival data were analyzed using The Cancer Genome Atlas (TCGA) data set and validated using NCBI Gene Expression Omnibus (NCBI-GEO) cohort. Hierarchical clustering, functional enrichment analyses, and immune infiltration analysis were applied to evaluate the distinctive immune statuses in colon cancer risk subgroups stratified by IDO1 and CD8A expression. Moreover, Multivariate Cox regression analysis and Receiver Operating Characteristic (ROC) analyses were conducted to determine the prognostic value of IDO1/CD8A stratification. The IDO1/CD8A classifier may be suitable for use in the prediction of cancer development. It was validated via an in vivo murine model.

Results

The stratification analysis demonstrated that the colon cancer subtype with the CD8AhighIDO1high* tumor resulted in the worst survival despite high levels of CD8 infiltrates. Its poor prognosis was associated with high levels of immune response, checkpoint genes, and Th1/IFN-γ gene signatures, regardless of CMS classification. Moreover, the IDO1/CD8A stratification was identified as an independent prognostic factor of overall survival (OS) and a useful predictive biomarker in colon cancer. In vivo data revealed the CD8AhighIDO1high group showed strong correlations with late-stage metastasis of colon carcinoma cells and upregulation of immune checkpoints.

Conclusions

The findings indicate that the proposed IDO1/CD8A stratification has exact and independent prognostic implications beyond CD8 T cell alone and CMS classification. As a result, it may represent a promising tool for risk stratification in colon cancer and improve the development of immunotherapies for patients with colon cancer in the future.
A Potent and Selective Dual Inhibitor of AXL and MERTK Possesses Both Immunomodulatory and Tumor-Targeted Activity
Authors: Rios-Doria Jonathan , Favata Margaret , Lasky Kerri , Feldman Patricia , Lo Yvonne , Yang Gengjie , Stevens Christina , Wen Xiaoming , Sehra Sarita , Katiyar Kamna , Liu Ke , Wynn Richard , Harris Jennifer J. , Ye Min , Spitz Susan , Wang Xiaozhao , He Chunhong , Li Yun-Long , Yao Wenqing , Covington Maryanne , Scherle Peggy , Koblish Holly
Published: December 2020

TYRO3, AXL, and MERTK constitute the TAM family of receptor tyrosine kinases, which play important roles in tumor growth, survival, cell adhesion, as well as innate immunity, phagocytosis, and immune-suppressive activity. Therefore, targeting both AXL and MERTK kinases may directly impact tumor growth and relieve immunosuppression. We describe here the discovery of INCB081776, a potent and selective dual inhibitor of AXL and MERTK that is currently in phase 1 clinical trials. In cellular assays, INCB081776 effectively blocked autophosphorylation of AXL or MERTK with low nanomolar half maximal inhibitory concentration values in tumor cells and Ba/F3 cells transfected with constitutively active AXL or MERTK. INCB081776 inhibited activation of MERTK in primary human macrophages and partially reversed M2 macrophage–mediated suppression of T-cell proliferation, which was associated with increased interferon-γ production. In vivo, the antitumor activity of INCB081776 was enhanced in combination with checkpoint blockade in syngeneic models, and resulted in increased proliferation of intratumoral CD4+ and CD8+ T cells. Finally, antitumor activity of INCB081776 was observed in a subset of sarcoma patient–derived xenograft models, which was linked with inhibition of phospho-AKT. These data support the potential therapeutic utility of INCB081776 as an immunotherapeutic agent capable of both enhancing tumor immune surveillance and blocking tumor cell survival mechanisms.
Ghost Interaction of Breathers
Authors: Xu Gang , Gelash Andrey , Chabchoub Amin , Zakharov Vladimir , Kibler Bertrand
Published: December 2020

Mutual interaction of localized nonlinear waves, e.g., solitons and modulation instability patterns, is a fascinating and intensively-studied topic of nonlinear science. Here we report the observation of a novel type of breather interaction in telecommunication optical fibers, in which two identical breathers propagate with opposite group velocities. Under controlled conditions, neither amplification nor annihilation occurs at the collision point and most interestingly, the respective envelope amplitude, resulting from the interaction, almost equals another envelope maximum of either oscillating and counterpropagating breather. This ghost-like breather interaction dynamics is fully described by anN-breather solution of the nonlinear Schrödinger equation.
Higher Incidence of Atrial Fibrillation in Left Ventricular-to-Right Atrial Shunt Patients
Authors: Chou Hongda , Chen Hongxia , Xie Juan , Xu Aiqing , Mu Guanyu , Han Fei , Tse Gary , Li Guangping , Liu Tong , Fu Huaying
Published: December 2020

Background: The possible association between atrial fibrillation (AF) and left ventricular-to-right atrial shunt (LVRAS) has never been reported yet. The present study investigated the incidence of AF in LVRAS.

Methods: This was a retrospective study of consecutive patients undergoing echocardiography at a single tertiary center. Clinical data, laboratory results and echocardiography parameters such as right atrial area (RAA), right ventricular end diastolic diameter (RVDD) and left atrial diameter (LAD) were compared between LVRAS group and non-LVRAS patients, and between AF and non-AF patients. Propensity score matching was performed to decrease the effect of confounders. Logistic regression analysis and mediation analysis were used to estimate the relationship between LVRAS and AF.

Results: A total of 3,436 patients were included, and the incidence of LVRAS was 1.16% (n = 40). The LVRAS group had significantly larger RAA, RVDD and LAD compared with non-LVRAS group. Those who suffered from AF showed larger RAA, RVDD and LAD compared with those who maintained sinus rhythm. Multivariable logistic regression showed that gender (OR: 0.608), age (OR: 1.048), LAD (OR: 1.111), mean pulmonary artery blood pressure (mPAP, OR: 1.023), TR (OR: 2.309) and LVRAS (OR: 12.217) were significant factors for AF. RAA could partially mediate the relationship between LVRAS and AF according to the result of mediation analysis.

Conclusions: Our study suggested that LVRAS, TR, LAD, mPAP, age and male were risk factors for AF. RA enlargement might underlie mechanism in the higher incidence of AF in LVRAS patients. These findings should be confirmed in larger prospective studies.
Cell Volume Changes and Membrane Ruptures Induced by Hypotonic Electrolyte and Sugar Solutions
Authors: Božič Bojan , Zemljič Jokhadar Špela , Kristanc Luka , Gomišček Gregor
Published: December 2020

The cell volume changes induced by hypotonic electrolyte and sucrose solutions were studied in Chinese-hamster-ovary epithelial cells. The effects in the solutions with osmolarities between 32 and 315 mosM/L and distilled water were analyzed using bright-field and fluorescence confocal microscopy. The changes of the cell volume, accompanied by the detachment of cells, the formation of blebs, and the occurrence of almost spherical vesicle-like cells (“cell-vesicles”), showed significant differences in the long-time responses of the cells in the electrolyte solutions compared with the sucrose-containing solutions. A theoretical model based on different permeabilities of ions and sucrose molecules and on the action of Na+/K+-ATPase pumps is applied. It is consistent with the observed temporal behavior of the cells’ volume and the occurrence of tension-induced membrane ruptures and explains lower long-time responses of the cells in the sucrose solutions.
The Acute Effects of 5 Fluorouracil on Skeletal Muscle Resident and Infiltrating Immune Cells in Mice
Authors: VanderVeen Brandon N. , Sougiannis Alexander T. , Velazquez Kandy T. , Carson James A. , Fan Daping , Murphy E. Angela
Published: December 2020

5 fluorouracil (5FU) has been a first-choice chemotherapy drug for several cancer types (e.g., colon, breast, head, and neck); however, its efficacy is diminished by patient acquired resistance and pervasive side effects. Leukopenia is a hallmark of 5FU; however, the impact of 5FU-induced leukopenia on healthy tissue is only becoming unearthed. Recently, skeletal muscle has been shown to be impacted by 5FU in clinical and preclinical settings and weakness and fatigue remain among the most consistent complaints in cancer patients undergoing chemotherapy. Monocytes, or more specifically macrophages, are the predominate immune cell in skeletal muscle which regulate turnover and homeostasis through removal of damaged or old materials as well as coordinate skeletal muscle repair and remodeling. Whether 5FU-induced leukopenia extends beyond circulation to impact resident and infiltrating skeletal muscle immune cells has not been examined. The purpose of the study was to examine the acute effects of 5FU on resident and infiltrating skeletal muscle monocytes and inflammatory mediators. Male C57BL/6 mice were given a physiologically translatable dose (35 mg/kg) of 5FU, or PBS, i.p. once daily for 5 days to recapitulate 1 dosing cycle. Our results demonstrate that 5FU reduced circulating leukocytes, erythrocytes, and thrombocytes while inducing significant body weight loss (>5%). Flow cytometry analysis of the skeletal muscle indicated a reduction in total CD45+ immune cells with a corresponding decrease in total CD45+CD11b+ monocytes. There was a strong relationship between circulating leukocytes and skeletal muscle CD45+ immune cells. Skeletal muscle Ly6cHigh activated monocytes and M1-like macrophages were reduced with 5FU treatment while total M2-like CD206+CD11c- macrophages were unchanged. Interestingly, 5FU reduced bone marrow CD45+ immune cells and CD45+CD11b+ monocytes. Our results demonstrate that 5FU induced body weight loss and decreased skeletal muscle CD45+ immune cells in association with a reduction in infiltrating Ly6cHigh monocytes. Interestingly, the loss of skeletal muscle immune cells occurred with bone marrow cell cycle arrest. Together our results highlight that skeletal muscle is sensitive to 5FU’s off-target effects which disrupts both circulating and skeletal muscle immune cells.
Foliar Cellulose and Lignin Degradation of Two Dominant Tree Species in a Riparian Zone of the Three Gorges Dam Reservoir, China
Authors: Chen Zhangting , Chen Xuemei , Wang Chaoying , Li Changxiao
Published: December 2020

The riparian zone can affect the degradation of foliar cellulose and lignin by changing the hydrological gradient of the foliage decomposition environment. However, their degradation dynamics during the process of foliage decomposition remain unclear in mixed plantation forests in a riparian zone. Herein, we explored degradation of cellulose and lignin for two dominant riparian species, bald cypress [Taxodium distichum (L.) Rich.] and Chinese willow (Salix matsudana Koidz.), and a combined treatment with equal proportions of foliar mass of these species, involving three water treatments [no submergence (CK), shallow submergence (SS), and deep submergence (DS)] in a riparian zone of the Three Gorges Dam Reservoir (TGDR), China. Throughout an entire year’s incubation, the degradation of cellulose and lignin was 55.57–97.76% and 79.87–93.82%, respectively. In the early stage of decomposition (i.e., the first 30 days), cellulose and lignin were rapidly degraded, and the mass loss of cellulose and lignin in water environments (SS and DS) was greater than that in a non-flooded environment (CK) regardless of the foliage type. The degradation of cellulose and lignin was mainly related to the quality of the leaf litter (as indicated by the concentrations of cellulose and lignin, and the contents of C, N, and P), decomposition period, and local environmental factors (temperature, water gradients, and dissolved oxygen). Our results will provide a clear insight into the material cycling process in a riparian zone of the TGDR and similar ecosystems in other regions.
Using the VIA Classification to Advance a Psychological Science of Virtue
Authors: McGrath Robert E. , Brown Mitch
Published: December 2020

The VIA Classification of Character Strengths and Virtue has received substantial attention since its inception as a model of 24 dimensions of positive human functioning, but less so as a potential contributor to a psychological science on the nature of virtue. The current paper presents an overview of how this classification could serve to advance the science of virtue. Specifically, we summarize previous research on the dimensional versus categorical characterization of virtue, and on the identification of cardinal virtues. We give particular attention to the three-dimensional model of cardinal virtues that includes moral, self-regulatory, and intellectual domains. We also discuss the possibility that these three clusters be treated as fundamental elements of a virtue model, meaning that they clearly and directly contribute to both individual and communal flourishing across various cultures. This discussion includes a summary of previous speculations about the evolution of adaptations underlying the human capacity for using behavioral repertoires associated with the three virtues, as well as discussing ways in which they simultaneously enhance community and individual, in the last case focusing particularly on evidence concerning mating potential. We then discuss the relationship between the evolutionary perspective on virtues and Aristotle’s concept of the reciprocity of the virtues. Finally, we provide speculations about the nature of practical wisdom. While accepting the potential value of future revisions to the VIA model, that model even under its current conditions has the potential to generate a number of intriguing and testable hypotheses about the nature of virtue.
Recommendations for Implementing Gamification for Mental Health and Wellbeing
Authors: Cheng Vanessa Wan Sze
Published: December 2020

Gamification is increasingly being proposed as a strategy to increase engagement for mental health and wellbeing technologies. However, its implementation has been criticized as atheoretical, particularly in relation to behavior change theory and game studies theories. Definitions of the term “gamification” vary, sometimes widely, between and within academic fields and the effectiveness of gamification is yet to be empirically established. Despite this, enthusiasm for developing gamified mental health technologies, such as interventions, continues to grow. There is a need to examine how best to implement gamification in mental health and wellbeing technologies in a way that takes quick production cycles into account while still emphasizing empirical investigation and building a rigorous evidence base. With reference to game studies and the medical (eHealth/mHealth) literature, this article interrogates gamification for mental health and wellbeing by examining core properties of the game form. It then explores how gamification can best be conceptualized and implemented for mental health and wellbeing goals from conceptualization through to iterative co-development and evaluation that accommodates software development schedules. Finally, it summarizes its conceptual analysis into recommendations for researchers and designers looking to do so. These recommendations are: (1) assess suitability, (2) implement to support, (3) assess acceptability, (4) evaluate impact, and (5) document comprehensively. These recommendations aim to encourage clear language, unified terminology, the application and evaluation of theory, comprehensive and constant documentation, and transparent evaluation of outcomes.
“All of Me Is Completely Different”: Experiences and Consequences Among Victims of Technology-Assisted Child Sexual Abuse
Authors: Joleby Malin , Lunde Carolina , Landström Sara , Jonsson Linda S.
Published: December 2020

The aim of the present study was to gain a first-person perspective on the experiences of technology-assisted child sexual abuse (TA-CSA), and a deeper understanding of the way it may affect its victims. Seven young women (aged 17–24) with experience of TA-CSA before the age of 18 participated in individual in-depth interviews. The interviews were teller-focused with the aim of capturing the interviewee’s own story about how they made sense of their experiences over time, and what impact the victimization had on them in the short and long terms. Thematic analysis of the interviews revealed a broad range of abusive experiences that had profoundly impacted the individuals’ lives, health and self-concepts. Three dominant themes emerged from the analysis –From thrilling to abusive,Negative effect on health and wellbeing, andA new self after the abuse.From thrilling to abusivecaptures the wide range of experiences described, starting from the child’s own sexual curiosity to descriptions of having been manipulated or threatened into engaging in sexual activity, as well as the sometimes long and complex process of understanding the severity of one’s experiences.Negative effect on health and wellbeingdescribes the victimization’s comprehensive impact on the life and health of the participants, how they blamed themselves for what had happened, and the struggle of having to live with the constant fear of pictures from the abuse resurfacing.A new self after the abusedepicts how the victimization impacted the way participants viewed and thought about themselves in relation to others, and distorted their views of their bodies. The findings are discussed in relation to previous research on both offline CSA and TA-CSA, as well as theoretical and practical implications.
Luteal Phase in Assisted Reproductive Technology
Authors: Tesarik Jan , Conde-López Cristina , Galán-Lázaro Maribel , Mendoza-Tesarik Raquel
Published: December 2020

Luteal phase (LP) is the period of time beginning shortly after ovulation and ending either with luteolysis, shortly before menstrual bleeding, or with the establishment of pregnancy. During the LP, the corpus luteum (CL) secretes progesterone and some other hormones that are essential to prepare the uterus for implantation and further development of the embryo, the function known as uterine receptivity. LP deficiency (LPD) can occur when the secretory activity of the CL is deficient, but also in cases of normal CL function, where it is caused by a defective endometrial response to normal levels of progesterone. LPD is particularly frequent in treatments using assisted reproductive technology (ART). Controlled ovarian stimulation usually aims to obtain the highest number possible of good-quality oocytes and requires the use of gonadotropin-releasing hormone (GnRH) analogs, to prevent premature ovulation, as well as an ovulation trigger to achieve timed final oocyte maturation. Altogether, these treatments suppress pituitary secretion of luteinizing hormone (LH), required for the formation and early activity of the CL. In addition to problems of endometrial receptivity for embryos, LPD also leads to dysfunction of the local uterine immune system, with an increased risk of embryo rejection, abnormally high uterine contractility, and restriction of uterine blood flow. There are two alternatives of LPD prevention: a direct administration of exogenous progesterone to restore the physiological progesterone serum concentration independently of the CL function, on the one hand, and treatments aimed to stimulate the CL activity so as to increase endogenous progesterone production, on the other hand. In case of pregnancy, some kind of LP support is often needed until the luteal–placental shift occurs. If LPD is caused by defective response of the endometrium and uterine immune cells to normal concentrations of progesterone, a still poorly defined condition, symptomatic treatments are the only available solution currently available.
Applying Citizen Science for Sustainable Development: Rainfall Monitoring in Western Nepal
Authors: Paul Jonathan D. , Cieslik Katarzyna , Sah Neeraj , Shakya Puja , Parajuli Binod Prasad , Paudel Saugat , Dewulf Art , Buytaert Wouter
Published: December 2020

We introduce a case-study agnostic framework for the application of citizen science in a sustainable development context. This framework is tested against an activity in two secondary schools in western Nepal. While the purpose of this activity is to generate locally relevant knowledge on the physical processes behind natural hazards, we concentrate here on its implementation, i.e., to obtain a better understanding of the dynamic of the activity and to learn how it should be implemented. We determined the social capital of secondary schools as a gateway to the local community: they provide a unique setting to bring different stakeholders together. We find that co-designing a teaching programme is an effective means of both complementing local curricula and ensuring continued buy-in of local stakeholders (i.e., teachers). Student engagement depends on the local relevance of teaching materials, with more holistic or global concepts, such as climate change of lesser importance. Our activity focused on rainfall, including student-led data collection. These rainfall data provide a very good fit to co-located rain gauge data, with an average difference on weekly readings of 11.8%, reducing to 8.3% when averaged over all student readings. The autonomous development of student-organized science clubs suggested that our original framework underestimated students' capacity to apply knowledge elsewhere creatively. These clubs may be used to obtain participant feedback to improve and tailor future activities. Quantitative assessment of long-term sustainability remains challenging, due in part to high levels of student turnover. We suggest that integrating scientists wherever possible within a school or local community has a direct and positive result on participant retention.
Maize-Pigeonpea Intercropping Outperforms Monocultures Under Drought
Authors: Renwick Leah L. R. , Kimaro Anthony A. , Hafner Johannes M. , Rosenstock Todd S. , Gaudin Amélie C. M.
Published: December 2020

There is an urgent need to develop resilient agroecosystems capable of helping smallholder farmers adapt to climate change, particularly drought. In East Africa, diversification of maize-based cropping systems by intercropping with grain and tree legumes may foster productivity and resilience to adverse weather conditions. We tested whether intercropping enhances drought resistance and crop and whole-system yields by imposing drought in monocultures and additive intercrops along a crop diversity gradient—sole maize (Zea mays), sole pigeonpea (Cajanus cajan), maize-pigeonpea, maize-gliricidia (Gliricidia sepium, a woody perennial), and maize-pigeonpea-gliricidia—with and without fertilizer application. We developed and tested a novel low-cost, above-canopy rainout shelter design for drought experiments made with locally-sourced materials that successfully reduced soil moisture without creating sizeable artifacts for the crop microenvironment. Drought reduced maize grain yield under fertilized conditions in some cropping systems but did not impact pigeonpea grain yield. Whole-system grain yield and theoretical caloric and protein yields in two intercropping systems, maize-pigeonpea and maize-gliricidia, were similar to the standard sole maize system. Maize-pigeonepea performed most strongly compared to other systems in terms of protein yield. Maize-pigeonpea was the only intercrop that consistently required less land than its corresponding monocultures to produce the same yield (Land Equivalent Ratio >1), particularly under drought. Despite intercropping systems having greater planting density than sole maize and theoretically greater competition for water, they were not more prone to yield loss with drought. Our results show that maize-pigeonpea intercropping provides opportunities to produce the same food on less land under drought and non-drought conditions, without compromising drought resistance of low-input smallholder maize systems.
Facial Reconstruction: A Systematic Review of Current Image Acquisition and Processing Techniques
Authors: Tarassoli Sam P. , Shield Matthew E. , Allen Rhian S. , Jessop Zita M. , Dobbs Thomas D. , Whitaker Iain S.
Published: December 2020

Introduction: Plastic and reconstructive surgery is based on a culmination of technological advances, diverse techniques, creative adaptations and strategic planning. 3D imaging is a modality that encompasses several of these criteria while encouraging the others. Imaging techniques used in facial imaging come in many different modalities and sub-modalities which is imperative for such a complex area of the body; there is a clear clinical need for hyper-specialized practice. However, with this complexity comes variability and thus there will always be an element of bias in the choices made for imaging techniques.

Aims and Objectives: The aim of this review is to systematically analyse the imaging techniques used in facial reconstruction and produce a comprehensive summary and comparison of imaging techniques currently available, including both traditional and novel methods.

Methods: The systematic search was performed on EMBASE, PubMed, Scopus, Web of Science and Cochrane reviews using keywords such as “image technique/acquisition/processing,” “3-Dimensional,” “Facial,” and “Reconstruction.” The PRISMA guidelines were used to carry out the systematic review. Studies were then subsequently collected and collated; followed by a screening and exclusion process with a final full-text review for further clarification in regard to the selection criteria. A risk of bias assessment was also carried out on each study systematically using the respective tool in relation to the study in question.

Results: From the initial 6,147 studies, 75 were deemed to fulfill all selection criteria and selected for meta-analysis. The majority of papers involved the use of computer tomography, though the use of magnetic resonance and handheld scanners using sonography have become more common in the field. The studies ranged in patient population, clinical indication. Seminal papers were highlighted within the group of papers for further analysis.

Conclusions: There are clearly many factors that affect the choice of image acquisition techniques and their potential at being ideal for a given role. Ultimately the surgical team's choice will guide much of the decision, but it is crucial to be aware of not just the diagnostic ability of such modalities, but their treatment possibilities as well.
Proximity Interactions in a Permanently Housed Dairy Herd: Network Structure, Consistency, and Individual Differences
Authors: Chopra Kareemah , Hodges Holly R. , Barker Zoe E. , Vázquez Diosdado Jorge A. , Amory Jonathan R. , Cameron Tom C. , Croft Darren P. , Bell Nick J. , Codling Edward A.
Published: December 2020

Understanding the herd structure of housed dairy cows has the potential to reveal preferential interactions, detect changes in behavior indicative of illness, and optimize farm management regimes. This study investigated the structure and consistency of the proximity interaction network of a permanently housed commercial dairy herd throughout October 2014, using data collected from a wireless local positioning system. Herd-level networks were determined from sustained proximity interactions (pairs of cows continuously within three meters for 60 s or longer), and assessed for social differentiation, temporal stability, and the influence of individual-level characteristics such as lameness, parity, and days in milk. We determined the level of inter-individual variation in proximity interactions across the full barn housing, and for specific functional zones within it (feeding, non-feeding). The observed networks were highly connected and temporally varied, with significant preferential assortment, and inter-individual variation in daily interactions in the non-feeding zone. We found no clear social assortment by lameness, parity, or days in milk. Our study demonstrates the potential benefits of automated tracking technology to monitor the proximity interactions of individual animals within large, commercially relevant groups of livestock.
Early-Life Dam-Calf Contact and Grazing Experience Influence Post-Weaning Behavior and Herbage Selection of Dairy Calves in the Short Term
Authors: Nicolao Alessandra , Coppa Mauro , Bouchon Matthieu , Sturaro Enrico , Pomiès Dominique , Martin Bruno , Koczura Madeline
Published: December 2020

Rearing dairy calves with their mothers could teach them how to graze, optimizing grass use, and improving their welfare and performance. We tested the short-term effects of dam-calf contact experience on grazing and social behavior of weaned calves, monitored over seven days for their first post-weaning grazing experience. “Dam” (D) calves were reared and grazed with their mothers until weaning. “Mixed” calves (M) were separated from their mothers after 4 ± 0.5 weeks, they experienced dam-calf contact, but not grazing. “Standard” (S) calves had never experienced either dam-calf contact (separated at birth) or grazing. Each group grazed an equivalent pasture plot offering heterogeneous herbage. Scan sampling of calves' activities was performed every 5 min, 6 h per day, on Days 0, 1, 2, 3, and 7. Daily, the time when calves started grazing after introduction to pasture, and the number and duration of their grazing cycles were measured. Daily activities were differentiated into ingestion, rumination, and idling. The proportion of time that calves spent grouped with other individuals or isolated, and standing or lying were recorded. When grazing, their bites were characterized by botanical family group, height of the selected bite and vegetation status. Individual average daily gains from the 2-week periods before and after grazing were calculated, and were equivalent between groups (313 ± 71 g/d). On Day 0, D-calves started grazing immediately (1 ± 4.1 min), unlike M- and S-calves (39 ± 4.1 and 23 ± 4.1 min), and D-calves grazed patches of dry grass 21.7 times less than M-calves and 16.9 times less than S-calves. Dry herbage patch preference and grazing start time differences disappeared on Day 1. Calves spent the same time ingesting and idling, but M-calves spent on average 1.6 times less ruminating than D- or S-calves. The D-calves showed grazing behavior similar to that of adult cows, selecting grasses throughout pasture utilization, although legumes and forbs were present in the grazed layer. On the contrary, M- and S-calves did not express any specific preference. The S-calves spent more time isolated but had more positive reciprocal interactions than the calves in the other groups.
Xylanase and Direct-Fed Microbials (DFM) Potential for Improvement of Live Performance, Energy Digestibility, and Reduction of Environmental Microbial Load of Broilers
Authors: Nusairat Basheer , Wang Jeng-Jie
Published: December 2020

The challenge of identifying alternatives to subtherapeutic levels of antibiotic growth promoters (AGP) in animal feed has led to increased interest in feed additives such as exogenous enzymes and direct-fed microbials (DFM). Six corn soy-based dietary treatments were designed to investigate the effect of high-efficiency xylanase alone, Bacillus spp. probiotics alone, and their combination vs. a commonly used antibiotic growth promoter (bacitracin methylene disalicylate; BMD) on live performance and environmental Clostridium perfringens load of broiler chickens with eight replicate pens per treatment. Diets were as follows: standard diet (positive control; PC); 130 kcal/kg reduced-energy diet (negative control; NC); NC with xylanase (NC + Xy); NC with probiotics (NC + Pro); NC with xylanase and probiotics mix (NC + XyPro); and NC with BMD (NC + BMD). Data were analyzed as one-way ANOVA. At 35 and 42 days, birds fed with NC + XyPro and NC + BMD were heavier (P < 0.05) than birds fed with NC. Improvement in feed conversion ratio (FCR) (P = 0.0001) was observed from 1 to 42 days by ~3 points in both NC + XyPro and NC + BMD compared to NC. The NC + XyPro reduced lesion scores by 66% compared to PC and NC. Litter C. perfringens cell count was reduced by ~16% with supplementation of XyPro or BMD. It can be concluded that a blend of xylanase (10 XU/g feed) and Bacillus spp. [1 × 105 colony forming units (CFU)/g feed] can be used as an alternative to AGP in low-energy broiler diets.
A Simulation-Based Optimization Method for Warehouse Worker Assignment
Authors: Ganbold Odkhishig , Kundu Kaustav , Li Haobin , Zhang Wei
Published: December 2020

The general assignment problem is a classical NP-hard (non-deterministic polynomial-time) problem. In a warehouse, the constraints on the equipment and the characteristics of consecutive processes make it even more complicated. To overcome the difficulty in calculating the benefit of an assignment and in finding the optimal assignment plan, a simulation-based optimization method is introduced. We first built a simulation model of the warehouse with the object-oriented discrete-event simulation (O2DES) framework, and then implemented a random neighborhood search method utilizing the simulation output. With this method, the throughput and service level of the warehouse can be improved, while keeping the number of workers constant. Numerical results with real data demonstrate the reduction of discrepancy between inbound and outbound service level performance. With a less than 10% reduction in inbound service level, we can achieve an over 30% increase in outbound service level. The proposed decision support tool assists the warehouse manager in dealing with warehouse worker allocation problem under conditions of random daily workload.
Feasibility of Kd-Trees in Gaussian Process Regression to Partition Test Points in High Resolution Input Space
Authors: De Boi Ivan , Ribbens Bart , Jorissen Pieter , Penne Rudi
Published: December 2020

Bayesian inference using Gaussian processes on large datasets have been studied extensively over the past few years. However, little attention has been given on how to apply these on a high resolution input space. By approximating the set of test points (where we want to make predictions, not the set of training points in the dataset) by a kd-tree, a multi-resolution data structure arises that allows for considerable gains in performance and memory usage without a significant loss of accuracy. In this paper, we study the feasibility and efficiency of constructing and using such a kd-tree in Gaussian process regression. We propose a cut-off rule that is easy to interpret and to tune. We show our findings on generated toy data in a 3D point cloud and a simulated 2D vibrometry example. This survey is beneficial for researchers that are working on a high resolution input space. The kd-tree approximation outperforms the naïve Gaussian process implementation in all experiments.