bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of Patients’ Quality of Life during Conservative Treatment after Distal Radius Fracture
Authors: Ratajczak Piotr , Meller Paweł , Kopciuch Dorota , Paczkowska Anna , Zaprutko Tomasz , Kus Krzysztof
Published: November 2022

Introduction: This study aimed to assess patients’ quality of life after distal radius fracture treatment (at least six months, but no more than ten years, after the treatment) based on the analysis of objective and subjective parameters and the influence of the fractured side on the final results. Materials and Methods: The study sample consisted of 30 women who claimed to be right-handed, divided depending on the side of the fracture (left vs. right limb). Patients were evaluated with a goniometer for active wrist movement, pronation, and supination in the elbow joint. Furthermore, the global grip strength of the upper limb was assessed using a dynamometer (Biometrics Ltd.) device, after which patients were asked to complete a wrist evaluation questionnaire (PRWE) and the Polish version of the SF-36 questionnaire assessing the quality of life. Results: There were statistically significant differences in the active movement of the wrist of the injured limb compared to the non-injured limb. In addition, inferior results were reported for injury of the right limb to those of the left. Conclusions: Injury of the right limb as opposed to injury of the left limb can have a negative impact on the assessment of quality of life in patients with right-limb dominance.
Revealing the Phenotypic and Genomic Background for PHA Production from Rapeseed-Biodiesel Crude Glycerol Using Photobacterium ganghwense C2.2
Authors: Lascu Irina , Tănase Ana Maria , Jablonski Piotr , Chiciudean Iulia , Preda Maria Irina , Avramescu Sorin , Irgum Knut , Stoica Ileana
Published: November 2022

Polyhydroxyalkanoates (PHA) are promising biodegradable and biocompatible bioplastics, and extensive knowledge of the employed bacterial strain’s metabolic capabilities is necessary in choosing economically feasible production conditions. This study aimed to create an in-depth view of the utilization of Photobacterium ganghwense C2.2 for PHA production by linking a wide array of characterization methods: metabolic pathway annotation from the strain’s complete genome, high-throughput phenotypic tests, and biomass analyses through plate-based assays and flask and bioreactor cultivations. We confirmed, in PHA production conditions, urea catabolization, fatty acid degradation and synthesis, and high pH variation and osmotic stress tolerance. With urea as a nitrogen source, pure and rapeseed-biodiesel crude glycerol were analyzed comparatively as carbon sources for fermentation at 20 °C. Flask cultivations yielded 2.2 g/L and 2 g/L PHA at 120 h, respectively, with molecular weights of 428,629 g/mol and 81,515 g/mol. Bioreactor batch cultivation doubled biomass accumulation (10 g/L and 13.2 g/L) in 48 h, with a PHA productivity of 0.133 g/(L·h) and 0.05 g/(L·h). Thus, phenotypic and genomic analyses determined the successful use of Photobacterium ganghwense C2.2 for PHA production using urea and crude glycerol and 20 g/L NaCl, without pH adjustment, providing the basis for a viable fermentation process.
Fascaplysin Induces Apoptosis and Ferroptosis, and Enhances Anti-PD-1 Immunotherapy in Non-Small Cell Lung Cancer (NSCLC) by Promoting PD-L1 Expression
Authors: Luo Lianxiang , Xu Guangxiang
Published: November 2022

Fascaplysin is a natural product isolated from sponges with a wide range of anticancer activities. However, the mechanism of fascaplysin against NSCLC has not been clearly studied. In this study, fascaplysin was found to inhibit migration by regulating the wnt/β-catenin signaling pathway and reversing the epithelial–mesenchymal transition phenotype. Further research showed that the anti-NSCLC effect of fascaplysin was mainly through the induction of ferroptosis and apoptosis. Fascaplysin-induced ferroptosis in lung cancer cells, evidenced by increased levels of ROS and Fe2+ and downregulation of ferroptosis-associated protein and endoplasmic reticulum stress, was involved in fascaplysin-induced ferroptosis. In addition, ROS was found to mediate fascaplysin-induced apoptosis. Fascaplysin significantly upregulated the expression of PD-L1 in lung cancer cells, and enhanced anti-PD-1 antitumor efficacy in a syngeneic mouse model. Therefore, these results suggest that fascaplysin exerts anticancer effects by inducing apoptosis and ferroptosis in vitro, and improving the sensitivity of anti-PD-1 immunotherapy in vivo. Fascaplysin is a promising compound for the treatment of NSCLC.
Association of LTA and SOD Gene Polymorphisms with Cerebral White Matter Hyperintensities in Migraine Patients
Authors: Ferroni Patrizia , Palmirotta Raffaele , Egeo Gabriella , Aurilia Cinzia , Valente Maria Giovanna , Spila Antonella , Pierallini Alberto , Barbanti Piero , Guadagni Fiorella
Published: November 2022

White matter hyperintensities (WMHs) in migraine could be related to inflammatory and antioxidant events. The aim of this study is to verify whether migraine patients with WMHs carry a genetic pro-inflammatory/pro-oxidative status. To test this hypothesis, we analyzed lymphotoxin alpha (LTA; rs2071590T and rs2844482G) and superoxide dismutase 1 (SOD1; rs2234694C) and 2 (SOD2; rs4880T) gene polymorphisms (SNPs) in 370 consecutive patients affected by episodic (EM; n = 251) and chronic (CM; n = 119) migraine and in unrelated healthy controls (n = 100). Brain magnetic resonance was available in 183/370 patients. The results obtained show that genotypes and allele frequencies for all tested SNPs did not differ between patients and controls. No association was found between single SNPs or haplotypes and sex, migraine type, cardiovascular risk factors or disorders. Conversely, the LTA rs2071590T (OR = 2.2) and the SOD1 rs2234694C (OR = 4.9) alleles were both associated with WMHs. A four-loci haplotype (TGCT haplotype: rs2071590T/rs2844482G/rs2234694C/rs4880T) was significantly more frequent in migraineurs with WMHs (7 of 38) compared to those without WMHs (4 of 134; OR = 8.7). We may, therefore, conclude by suggesting that that an imbalance between pro-inflammatory/pro-oxidative and antioxidant events in genetically predisposed individuals may influence the development of WMHs.
GmWRI1c Increases Palmitic Acid Content to Regulate Seed Oil Content and Nodulation in Soybean (Glycine max)
Authors: Zheng Haowei , Zhao Duo , Shao Wentao , Lu Yun , Wang Wenhui , Hu Yanjiao , Li Jiajia , Zhu Shangshang , Wang Xiaobo
Published: November 2022

Soybean (Glycine max) is an important oil crop, but the regulatory mechanisms underlying seed oil accumulation remain unclear. We identified a member of the GmWRI1s transcription factor family, GmWRI1c, that is involved in regulating soybean oil content and nodulation. Overexpression of GmWRI1c in soybean hairy roots increased the expression of genes involved in glycolysis and de novo lipogenesis, the proportion of palmitic acid (16:0), and the number of root nodules. The effect of GmWRI1c in increasing the number of root nodules via regulating the proportion of palmitic acid was confirmed in a recombinant inbred line (RIL) population. GmWRI1c shows abundant sequence diversity and has likely undergone artificial selection during domestication. An association analysis revealed a correlation between seed oil content and five linked natural variations (Hap1/Hap2) in the GmWRI1c promoter region. Natural variations in the GmWRI1c promoter were strongly associated with the GmWRI1c transcript level, with higher GmWRI1c transcript levels in lines carrying GmWRI1cHap1 than in those carrying GmWRI1cHap2. The effects of GmWRI1c alleles on seed oil content were confirmed in natural and RIL populations. We identified a favourable GmWRI1c allele that can be used to breed new varieties with increased seed oil content and nodulation.
3D Molecular Imaging of Stratum Corneum by Mass Spectrometry Suggests Distinct Distribution of Cholesteryl Esters Compared to Other Skin Lipids
Authors: Sjövall Peter , Gregoire Sebastien , Wargniez William , Skedung Lisa , Luengo Gustavo S.
Published: November 2022

The crucial barrier properties of the stratum corneum (SC) depend critically on the design and integrity of its layered molecular structure. However, analysis methods capable of spatially resolved molecular characterization of the SC are scarce and fraught with severe limitations, e.g., regarding molecular specificity or spatial resolution. Here, we used 3D time-of-flight secondary ion mass spectrometry to characterize the spatial distribution of skin lipids in corneocyte multilayer squams obtained by tape stripping. Depth profiles of specific skin lipids display an oscillatory behavior that is consistent with successive monitoring of individual lipid and corneocyte layers of the SC structure. Whereas the most common skin lipids, i.e., ceramides, C24:0 and C26:0 fatty acids and cholesteryl sulfate, are similarly organized, a distinct 3D distribution was observed for cholesteryl oleate, suggesting a different localization of cholesteryl esters compared to the lipid matrix separating the corneocyte layers. The possibility to monitor the composition and spatial distribution of endogenous lipids as well as active drug and cosmetic substances in individual lipid and corneocyte layers has the potential to provide important contributions to the basic understanding of barrier function and penetration in the SC.
LPS-Induced Inhibition of miR-143 Expression in Brown Adipocytes Promotes Thermogenesis and Fever
Authors: Liu Jie , Zeng Dewei , Luo Junyi , Wang Huan , Xiong Jiali , Chen Xingping , Chen Ting , Sun Jiajie , Xi Qianyun , Zhang Yongliang
Published: November 2022

Fever is an important part of inflammatory response to infection. Although brown adipose tissue (BAT) thermogenesis is known to be potently influenced by systemic inflammation, the role of BAT during infection-induced fever remains largely unknown. Here, we injected mice with a low dose of LPS and found that low-dose LPS can directly induce thermogenesis of brown adipocytes. It is known that miR-143 is highly expressed in the BAT, and miR-143 knockout mice exhibited stronger thermogenesis under cold exposure. Interestingly, miR-143 was negatively correlated with an LPS-induced increase of TNFα and IL-6 mRNA levels, and the IL-6 pathway may mediate the inhibition of miR-143 expression. Moreover, miR-143 is down-regulated by LPS, and overexpression of miR-143 in brown adipocytes by lentivirus could rescue the enhancement of UCP1 protein expression caused by LPS, hinting miR-143 may be an important regulator of the thermogenesis in brown adipocytes. More importantly, the knockout of miR-143 further enhanced the LPS-induced increase of body temperature and BAT thermogenesis, and this result was further confirmed by in vitro experiments by using primary brown adipocytes. Mechanistically, adenylate cyclase 9 (AC9) is a new target gene of miR-143 and LPS increases BAT thermogenesis by a way of inhibiting miR-143 expression, a negative regulator for AC9. Our study considerably improves our collective understanding of the important function of miR-143 in inflammatory BAT thermogenesis.
Freshwater Macroalgae, Oedogonium, Grown in Wastewater Reduce Diet-Induced Metabolic Syndrome in Rats
Authors: Panchal Sunil K. , Ghattamaneni Naga K. R. , Magnusson Marie , Cole Andrew , Roberts David , Neveux Nicolas , Brown Lindsay , Paul Nicholas A.
Published: November 2022

Macroalgae produce compounds with industrial, pharmaceutical and nutritional applications. In this study, biomass from the freshwater macroalgal genus Oedogonium was grown in either treated municipal wastewater (M) or ash dam water from a coal-fired power station (D). The biomass was investigated for its metabolic responses in high-carbohydrate, high-fat diet-fed rats, a model of human metabolic syndrome. The Oedogonium biomass cultured in M contained higher amounts of K, Mg, omega-3 polyunsaturated fatty acids (PUFA), insoluble fibre and β-carotene, while biomass grown in D contained higher amounts of Al, Fe, V, Zn, Mn and As. Biomass from M further increased body weight and inflammation in the heart and colon in high-carbohydrate, high-fat diet-fed rats. In contrast, biomass from D prevented changes in metabolic, cardiovascular and liver parameters without changing tissue histology. We suggest that increased intake of metals and metalloids through macroalgal biomass from D may decrease abdominal fat deposition while polysaccharides, PUFA and carotenoids from M may improve blood glucose responses in an obesogenic diet. Thus, macroalgal biomass grown in different wastewater sources could be acceptable for feed or food applications. This biomass could even provide potential health benefits in diet-induced metabolic syndrome.
Targeting Oncogenic Rewiring of Lipid Metabolism for Glioblastoma Treatment
Authors: Lee Haksoo , Kim Dahye , Youn BuHyun
Published: November 2022

Glioblastoma (GBM) is the most malignant primary brain tumor. Despite increasing research on GBM treatment, the overall survival rate has not significantly improved over the last two decades. Although recent studies have focused on aberrant metabolism in GBM, there have been few advances in clinical application. Thus, it is important to understand the systemic metabolism to eradicate GBM. Together with the Warburg effect, lipid metabolism has emerged as necessary for GBM progression. GBM cells utilize lipid metabolism to acquire energy, membrane components, and signaling molecules for proliferation, survival, and response to the tumor microenvironment. In this review, we discuss fundamental cholesterol, fatty acid, and sphingolipid metabolism in the brain and the distinct metabolic alterations in GBM. In addition, we summarize various studies on the regulation of factors involved in lipid metabolism in GBM therapy. Focusing on the rewiring of lipid metabolism will be an alternative and effective therapeutic strategy for GBM treatment.
Synthesis, Structure and Stereochemistry of Dispirocompounds Based on Imidazothiazolotriazine and Pyrrolidineoxindole
Authors: Izmest’ev Alexei N. , Karnoukhova Valentina A. , Larin Alexander A. , Kravchenko Angelina N. , Fershtat Leonid L. , Gazieva Galina A.
Published: November 2022

Methods for the synthesis of two types of isomeric dispirocompounds based on imidazothiazolotriazine and pyrrolidineoxindole, differing in the structure of imidazothiazolotriazine fragment, namely, linear dispiro[imidazo[4,5-e]thiazolo[3,2-b][1,2,4]triazine-6,3′-pyrrolidine- 4′,3″-indolines] and angular dispiro[imidazo[4,5-e]thiazolo[2,3-c][1,2,4]triazine-7,3′-pyrrolidine-4′,3″-indolines] were proposed. The first method relies on a 1,3-dipolar cycloaddition of azomethine ylides generated in situ from paraformaldehyde and N-alkylglycine derivatives to the corresponding oxindolylidene derivatives of imidazothiazolotriazine. The cycloaddition leads to a mixture of two diastereomers resulted from anti- and syn-approaches of azomethine ylide in approximately a 1:1 ratio, which were separated by column chromatography. Another method consists in rearrangement of linear dispiro[imidazo[4,5-e]thiazolo[3,2-b][1,2,4]triazine-6,3′-pyrrolidine-4′,3″-indolines] into hitherto unavailable angular dispiro[imidazo[4,5-e]thiazolo[2,3-c]-[1,2,4]triazine-7,3′-pyrrolidine-4′,3″-indolines] upon treatment with KOH. It was found that the anti-diastereomer of linear type underwent rearrangement into the isomeric angular syn-diastereomer, while the rearrangement of the linear syn-diastereomer gave the angular anti-diastereomer.
Crack Detection and Classification in Moroccan Pavement Using Convolutional Neural Network
Authors: Hammouch Wafae , Chouiekh Chaymae , Khaissidi Ghizlane , Mrabti Mostafa
Published: November 2022

Crack is a condition indicator of the pavement’s structure. Generally, crack detection is an essential task for effective diagnosis of the road network. Moreover, evaluation of road quality is necessary to ensure traffic security. Since 2011, a periodic survey of approximately 57,500 km of Moroccan roads has been performed using an inspection vehicle (SMAC) which is equipped with high resolution cameras and GPS/DGPS receivers. Until recently, the teams of the National Center for Road Studies and Research (CNER) analyzed road surface states by visualization of pavement surface image sequences captured by the Multifunctional Pavement Assessment System (SMAC) in order to detect defects in road surfaces and classify them according to their type. However, this method involves manual processing and is complex, time consuming and subjective. In this paper, we propose an automated methodology for crack detection and classification in Moroccan flexible pavements using Convolutional Neural Networks (CNN). Transfer learning is also applied by testing a pre-trained Visual Geometry Group 19 (VGG-19) model. For the dataset used in this paper, the results indicate that good crack detection and classification are achieved using both models.
Redshifting and Blueshifting of β82 Chromophores in the Phycocyanin Hexamer of Porphyridium purpureum Phycobilisomes Due to Linker Proteins
Authors: Kikuchi Hiroto
Published: November 2022

Phycobilisomes in cyanobacteria and red algae are large protein complexes that absorb light and transfer energy for use in photosynthesis. The light energy absorbed by chromophores binding to phycobiliproteins in the peripheral rods can be funneled to the core through chromophores at very high efficiency. The molecular mechanism of excitation energy transfer within a phycobilisome is an example of a higher and unique function in a living organism. However, the mechanism underlying the high efficiency remains unclear. Thus, this study was carried out as a step to resolve this mechanism theoretically. The three-dimensional structure of phycobilisomes containing the linker proteins of the red alga Porphyridium purpureum was determined by cryoelectron microscopy at 2.82 Å resolution in 2020. Using these data, the absorption wavelength of each β82 chromophore in the phycocyanin hexamer located next to the core was calculated using quantum chemical treatment, considering the electric effect from its surrounding phycocyanin proteins and two linker proteins. In addition to unaffected chromophores, chromophores that were redshifted and blueshifted under the electrical influence of the two linker proteins were found. Namely, the chromophore serving as the energy sink in the rod was determined.
Y-TZP Physicochemical Properties Conditioned with ZrO2 and SiO2 Nanofilms and Bond Strength to Dual Resin Cement
Authors: Ribeiro Ricardo Faria , Oliveira Danilo Flamini , Tovani Camila Bussola , Ramos Ana Paula , Borges Ana Flavia Sanches , Faria Adriana Claudia Lapria , Almeida Rossana Pereira de , Rodrigues Renata Cristina Silveira
Published: November 2022

Commercial Yttria-tetragonal zirconia polycrystalline (Y-TZP) was subjected to surface treatments, and the bond strength of dual resin cement to Y-TZP and failure modes were evaluated. Disks (12 mm × 2 mm), cylinders (7 mm × 3.3 mm), and bars (25 mm × 5 mm × 2 mm) were milled from Y-TZP CAD-CAM blocks, divided into seven groups, and subjected to different surface treatments; silicatization was used as control. On the basis of the literature, this study evaluated modifications with films containing SiO2 nanoparticles and silane; SiO2+ZrO2—SiO2 (50%) and ZrO2 (50%) nanoparticles, SiO2+ZrO2/Silane-SiO2 (50%) and ZrO2 (50%) nanoparticles, and silane. Specimens were analyzed by wettability (n = 3), surface free energy (n = 3), X-ray diffraction (n = 1), Fourier transform infrared spectroscopy (FTIR) (n = 1), roughness (n = 5), shear bond test (n = 10), and dynamic modulus (n = 3). Specimens treated with hydrofluoric acid—HF 40% presented significantly higher contact angle and lowest surface free energy (p < 0.05). The SiO2/Silane presented crystalline SiO2 on the surface. The surface roughness was significantly higher for groups treated with nanofilms (p < 0.05). Shear bond strength was significantly higher for silicatization, HF 40%/silicatization, SiO2/Silane, and SiO2+ZrO2/Silane groups. The proposed treatments with nanofilms had potentially good results without prejudice to the physicochemical characteristics of zirconia. Generally, groups that underwent silica surface deposition and silanization had better bond strength (p < 0.005).
Role of Carbon Phase in the Formation of Foam Glass Porous Structure
Authors: Goltsman Boris M. , Yatsenko Elena A.
Published: November 2022

The production of durable, non-combustible, heat-insulating materials is currently very important. One of the most promising materials is foam glass. Modern enterprises widely use organic foaming agents in foam glass production. The purpose of this work is to study the role of the carbon phase formed during the organic foaming agent’s (glycerol) thermal destruction in the processes of glass mass foaming. The samples were synthesized using the powder method with high-temperature treatment. Different ratios of glycerol and waterglass in a foaming mixture showed that amount of glycerol should be less than in waterglass. Otherwise, the amount is excessive and the glycerol burns out. It was shown that the quantitative description of the carbon phase structure and properties is complicated by its nanometer size and fusion into the glass. Theoretical calculations demonstrate that carbon particle size cannot be greater than 535 nm. Using a set of methods, it was proved that the carbon phase is represented by nanometer particles of amorphous sp2-carbon. Therefore, the foaming mechanism includes nanoparticles settling and immersing into the glass surface, a reaction of carbon with the sulfate ions from glass with a release of gases. Conclusions on foaming intensification via using sulfur additions and other organic foaming agents were drawn.
Research on Impermeability of Underwater Non-Dispersible Concrete in Saline Soil
Authors: Guo Baolin , Wang Chao , Ma Xianghui , Jiang Ruishuang , Wang Baomin , Yan Jifei , Liu Hang
Published: November 2022

The permeability of different strength grades of submerged non-dispersible concrete with different granulated slag admixtures in a saline soil environment simulated by different erosion solutions was investigated. The variation patterns of the chloride ion diffusion coefficient and pore characteristics were tested using NEL and MIP. The microscopic morphology of the specimens in different erosion environments and with slag doping was observed using SEM. The results showed that the impermeability of concrete in sulfate and complex salt environments was significantly reduced. The resistance of concrete to chloride ion penetration increased with the increase in strength grade, and the Cl− diffusion coefficient of C35 was 5–30% lower than those of C30 and C25 underwater non-dispersible concrete at 360 d. Meanwhile, the admixture of granulated blast-furnace slag optimized the pore size distribution and improved the matrix compactness and permeability.
Prolonged Use of Surgical Masks and Respirators Affects the Protection and Comfort for Healthcare Workers
Authors: Guan Xiaoning , Lin Jing , Han Jiaxiang , Gao Xiaodong , Zhang Ying , Hu Bijie , Guidoin Robert , Wang Lu
Published: November 2022

This study explored the ideal period for wearing masks to prevent the physiological and psychological problems associated with long-term face mask use during respiratory infections by healthcare workers. Breathing simulators, surgical masks (SM) and medical respirators (PM) were prepared for two to eight hours. Changes in the comfort of masks (facial skin temperature, breathing resistance, and moisture permeability) and protection (filtration efficiency, resistance to blood penetration, and colony count) were assessed. The results demonstrated that the masks offered efficient liquid-particle filtering even after eight hours of use. However, the number of bacterial colonies using PM and SM grew significantly after two and four hours, respectively. Concerning comfort, the inspiratory resistance of masks rose dramatically after two hours, whereas the moisture permeability declined considerably after four hours. In addition, skin temperature had a significant increase within two hours, which may result in facial discomfort. When conditions permitted, the hospital staff was instructed to replace their masks every two hours.
Behavior of Concrete Reinforced with Date Palm Fibers
Authors: Althoey Fadi , Hakeem Ibrahim Y. , Hosen Md. Akter , Qaidi Shaker , Isleem Haytham F. , Hadidi Haitham , Shahapurkar Kiran , Ahmad Jawad , Ali Elias
Published: November 2022

In recent decades, researchers have begun to investigate innovative sustainable construction materials for the development of greener and more environmentally friendly infrastructures. The main purpose of this article is to investigate the possibility of employing date palm tree waste as a natural fiber alternative for conventional steel and polypropylene fibers (PPFs) in concrete. Date palm fibers are a common agricultural waste in Middle Eastern nations, particularly Saudi Arabia. As a result, this research examined the engineering properties of high-strength concrete using date palm fibers, as well as the performance of traditional steel and PPF concrete. The concrete samples were made using 0.0%, 0.20%, 0.60%, and 1.0% by volume of date palm, steel, and polypropylene fibers. Ten concrete mixtures were made in total. Compressive strength, flexural strength, splitting tensile strength, density, ultrasonic pulse velocity (UPV), water absorption capability, and water permeability tests were performed on the fibrous-reinforced high-strength concrete. With a 1% proportion of date palm, steel, and polypropylene fibers, the splitting tensile strength improved by 17%, 43%, and 16%, respectively. By adding 1% fiber, flexural strength was increased by 60% to 85%, 67% to 165%, and 61% to 79%. In addition, date palm fibers outperformed steel and PPFs in terms of density, UPV, and water permeability. As a result, date palm fibers might potentially be employed in the present construction sector to improve the serviceability of structural elements.
Scintillation Characteristics of the Single-Crystalline Film and Composite Film-Crystal Scintillators Based on the Ce3+-Doped (Lu,Gd)3(Ga,Al)5O12 Mixed Garnets under Alpha and Beta Particles, and Gamma Ray Excitations
Authors: Mares Jiri A. , Gorbenko Vitalii , Kucerkova Romana , Prusa Petr , Beitlerova Alena , Zorenko Tetiana , Pokorny Martin , Witkiewicz-Łukaszek Sandra , Syrotych Yurii , D’Ambrosio Carmelo , Nikl Martin , Sidletskiy Oleg , Zorenko Yuriy
Published: November 2022

The crystals of (Lu,Gd)3(Ga,Al)5O12 multicomponent garnets with high density ρ and effective atomic number Zeff are characterized by high scintillation efficiency and a light yield value up to 50,000 ph/MeV. During recent years, single-crystalline films and composite film/crystal scintillators were developed on the basis of these multicomponent garnets. These film/crystal composites are potentially applicable for particle identification by pulse shape discrimination due to the fact that α-particles excite only the film response, γ-radiation excites only the substrate response, and β-particles excite both to some extent. Here, we present new results regarding scintillating properties of selected (Lu,Gd)3(Ga,Al)5O12:Ce single-crystalline films under excitation by alpha and beta particles and gamma ray photons. We conclude that some of studied compositions are indeed suitable for testing in the proposed application, most notably Lu1.5Gd1.5Al3Ga2O12:Ce film on the GAGG:Ce substrate, exhibiting an α-particle-excited light yield of 1790–2720 ph/MeV and significantly different decay curves excited by α- and γ-radiation.
Experimental Study on the Effect of Micro-Texture on EHL Point-Contact Film Thickness Subject to Sliding Conditions
Authors: Sun Jianxin , Bai Linqing , Guo Feng , Khan Zulfiqar Ahmad
Published: November 2022

Processing texture on contact surfaces can improve the friction performance of mechanical comments. In this research, micro-dimple textures with various parameter were processed on a steel ball’s surface with a picosecond laser. Then, the EHL (elastohydrodynamic lubrication) oil film thickness was measured on an optical ball-on-disc tribometer subjected to pure sliding conditions. The effects of sliding velocity, load, dimple location and dimple depth on the film thickness were analyzed. The results showed that the dimple affected the pressure distribution in the contact area, which in turn changed the distribution of the local film thickness. An increase in the local film thickness occurred between the dimple and outlet of the contact region, while a decrease in the film thickness formed from the dimple to the entrance of the contact area and both sides of the dimple’s edge. Velocity and applied loads affected the film thickness distribution as well. As the sliding velocity increased, the film thickness increasing region enlarged, while the film thickness-decreasing area shrank. The increase in load resulted in a negative effect on the increase in film thickness. This study will provide a reference for point-contact designs with low friction conditions.
Design and Fabrication of Mature Engineered Pre-Cardiac Tissue Utilizing 3D Bioprinting Technology and Enzymatically Crosslinking Hydrogel
Authors: Iwanaga Shintaroh , Hamada Yuta , Tsukamoto Yoshinari , Arai Kenichi , Kurooka Taketoshi , Sakai Shinji , Nakamura Makoto
Published: November 2022

The fabrication of mature engineered cardiac tissue is one of the major challenges in cardiac tissue engineering. For this purpose, we attempted to apply the 3D bioprinting approach. Aiming to construct an oriented tissue, a fine fiber-shaped scaffold with a support structure was first designed using CAD software. Then, a 3D bioprinter and cell-adhesive bio-inks were utilized to fabricate this structure. The cell-adhesive bio-inks were synthesized by combining sodium alginate and gelatin with tyramine, respectively, to form pre-gel materials that allow enzymatic crosslinking by horseradish peroxidase. By absorbance measurements, we confirmed that the tyramine modification rate of each polymer was 0.535 mmol/g-alginate and 0.219 mmol/g-gelatin. The width of the fiber-shaped scaffold was 216.8 ± 24.3 μm for the fabricated scaffold, while the design value was 200 μm. After 3D printing and adhesion-adding treatment of the scaffold with these bio-ink materials, cardiomyocytes were seeded and cultured. As a result, the cells spread onto the scaffold, and the entire pre-tissue contracted synchronously by day 6 of culture, showing a greater pulsatility than in the early days. Video analysis showed that the beating rate of pre-myocardial tissue on day 6 was 31 beats/min. In addition, we confirmed that the cardiomyocytes partially elongated along the long axis of the fiber-shaped scaffold in the pre-tissue cultured for 15 days by staining actin, suggesting the possibility of cell orientation. Furthermore, treatment with adrenaline resulted in a 7.7-fold increase in peak beating rate compared to that before treatment (from 6 beats/min to 46 beats/min), confirming the responsiveness of the pre-tissues to the drug. These results indicate that 3D bioprinting effectively produces mature cultured myocardial tissue that is oriented, contracts synchronously, and is responsive to drugs.
High-Strain-Rate Compression of Elastomers Subjected to Temperature and Humidity Conditions
Authors: Gkouti Elli , Chaudhry Muhammad Salman , Yenigun Burak , Czekanski Aleksander
Published: November 2022

Elastomers exhibit a complex response to high-strain-rate deformation due to their viscoelastic behaviour. Environmental conditions highly impact this behaviour, especially when both temperature and humidity change. In several applications where elastomers are used, the quantity of real humidity might vary, especially when the temperature is elevated. In the current research, elastomeric materials were subjected to high-strain-rate compression in various elevated and lowered (cold) temperatures. Different humidity levels were applied at room and elevated temperatures to analyze the behaviour of rubbers in dry and moist conditions. Results showed that the mechanical behaviour of rubbers is highly affected by any environmental change. In particular, the impact caused by humidity variations is relative to their ability to absorb or repel water on their surface.
Using an Artificial Neural Network for Improving the Prediction of Project Duration
Authors: Lishner Itai , Shtub Avraham
Published: November 2022

One of the most challenging tasks in project management is estimating the duration of a project. The unknowns that accompany projects, the different risks, the uniqueness of each project, and the differences between organizations’ culture and management techniques, hinder the ability to build one project duration prediction tool that can fit all types of projects and organizations. When machine learning (ML) techniques are used for project duration prediction, the challenge is even greater, as each organization has a different dataset structure, different features, and different quality of data. This hinders the ability to create one ML model that fits all types of organizations. This paper presents a new dynamic ML tool for improving the prediction accuracy of project duration. The tool is based on an artificial neural network (ANN) which is automatically adapted and optimized to different types of prediction methods and different datasets. The tool trains the ANN model multiple times with different architectures and uses a genetic algorithm to eventually choose the architecture which gives the most accurate prediction results. The validation process of the prediction accuracy is performed by using real-life project datasets supplied by two different organizations which have different project management approaches, different project types, and different project features. The results show that the proposed tool significantly improved the prediction accuracy for both organizations despite the major differences in the size, type, and structure of their datasets.
Structure of the Lipooligosaccharide from the Deep-Sea Marine Bacterium Idiomarina zobellii KMM 231T, Isolated at a Depth of 4000 Meters
Authors: Kokoulin Maxim S. , Dmitrenok Pavel S. , Romanenko Lyudmila A.
Published: November 2022

The structural characterization of lipopolysaccharides has critical implications for some biomedical applications, and marine bacteria are an inimitable source of new glyco-structures potentially usable in medicinal chemistry. On the other hand, lipopolysaccharides of marine Gram-negative bacteria present certain structural features that can help the understanding of the adaptation processes. The deep-sea marine Gram-negative bacterium Idiomarina zobellii KMM 231T, isolated from a seawater sample taken at a depth of 4000 m, represents an engaging microorganism to investigate in terms of its cell wall components. Here, we report the structural study of the R-type lipopolysaccharide isolated from I. zobellii KMM 231T that was achieved through a multidisciplinary approach comprising chemical analyses, NMR spectroscopy, and MALDI mass spectrometry. The lipooligosaccharide turned out to be characterized by a novel and unique pentasaccharide skeleton containing a very short mono-phosphorylated core region and comprising terminal neuraminic acid. The lipid A was revealed to be composed of a classical disaccharide backbone decorated by two phosphate groups and acylated by i13:0(3-OH) in amide linkage, i11:0 (3-OH) as primary ester-linked fatty acids, and i11:0 as a secondary acyl chain.
Antibacterial Mechanism of Chitosan–Gentamicin and Its Effect on the Intestinal Flora of Litopenaeus vannamei Infected with Vibrio parahaemolyticus
Authors: Li Lefan , Liang Fengyan , Li Chengpeng , Hou Tingting , Xu Dong-an
Published: November 2022

To explore the application of chitosan–gentamicin conjugate (CS-GT) in inhibiting Vibrio parahaemolyticus (V. parahaemolyticus), which is an important pathogen in aquatic animals worldwide, the antimicrobial activity of CS-GT and the effects of a CS-GT dose on the intestine histopathology and intestinal flora of V. parahaemolyticus-infected shrimps were explored. The results showed that CS-GT possessed broad-spectrum antibacterial activity, with minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and half inhibitory concentration (IC50) of 20.00 ± 0.01, 75.00 ± 0.02 and 18.72 ± 3.17 μg/mL for V. parahaemolyticus, respectively. Further scanning electron microscope and cell membrane damage analyses displayed that the electrostatic interaction of CS-GT with cell membrane strengthened after CS grafted GT, resulting in leakage of nucleic acid and electrolytes of V. parahaemolyticus. On the other hand, histopathology investigation indicated that high (100 mg/kg) and medium (50 mg/kg) doses of CS-GT could alleviate the injury of a shrimp’s intestine caused by V. parahaemolyticus. Further 16S rRNA gene sequencing analysis found high and medium dose of CS-GT could effectively inhabit V. parahaemolyticus invasion and reduce intestinal dysfunction. In conclusion, CS-GT possesses good antibacterial activity and could protect shrimps from pathogenic bacteria infection.
Surgical, Dermatological and Morphopathological Considerations in the Diagnosis and Treatment of Cutaneous Lymphoproliferative Tumors, Primary CD4+
Authors: Ardeleanu Valeriu , Moroianu Lavinia-Alexandra , Sava Anca , Tebeica Tiberiu , Jecan Radu Cristian , Moroianu Marius , Tatu Alin Laurentiu
Published: November 2022

Primary cutaneous lymphomas are a heterogeneous group of T-cell (CTCL) and B-cell lymphomas (CBCL) developing in the skin and without signs of extracutaneous disease at the time of diagnosis. The term “primary small/medium CD4+ T-cell lymphoma” was changed to “primary small/medium cutaneous CD4+ lymphoproliferative disorder” due to its indolent clinical behavior and uncertain malignant potential. This paper presents a rare case of primary cutaneous lymphoma with small to medium CD4+ T-cells. A 37-year-old patient presented with a tumor in the frontal region that had occurred approximately 8–9 months earlier. The tumor had a diameter of about 8–9 mm, well demarcated macroscopically, it was round in shape, about 6–7 mm high, pink in color, firm in consistency and painless during palpation. Surgical excision of the tumor was performed with a margin of safety of 8 mm and deep to the level of the frontal muscle fascia. The histopathological examination supported the diagnosis of cutaneous lymphoproliferation with a nodular disposition in the reticular dermis and extension around the follicular epithelia and sweat glands, composed mainly of dispersed medium-large lymphocytes. Additional immunohistochemical examination was requested. Immunohistochemical examination confirmed the diagnosis of “primary cutaneous CD4+ small/medium T-cell lymphoproliferative disorder.” Patient monitoring was carried out through clinical dermatological controls at 3, 6, and 12 months. After one year, a cranio-cerebral MRI was performed. For the following 5 years, an annual dermatological examination accompanied by cranio-cerebral MRI, blood count, and pulmonary X-ray were recommended. Similarly to all solitary skin lesions, the prognosis is excellent in this case, the only treatment being surgical excision.
Zinc Chloride Enhances the Antioxidant Status, Improving the Functional and Structural Organic Disturbances in Streptozotocin-Induced Diabetes in Rats
Authors: Anton Irina Claudia , Mititelu-Tartau Liliana , Popa Eliza Gratiela , Poroch Mihaela , Poroch Vladimir , Pelin Ana-Maria , Pavel Liliana Lacramioara , Drochioi Ilie Cristian , Botnariu Gina Eosefina
Published: November 2022

Background and Objectives: Diabetes mellitus (DM) is a complex disease affecting the whole metabolic balance of the body and resulting in multiple organ complications: cardiovascular, neuronal, renal, etc. Our study focuses on investigating the effect of zinc chloride (Zn) on certain blood parameters suggestive for assessing the metabolic disturbances, the liver and kidney function, the oxidative stress and the immune defense capacity in experimental-induced DM with streptozotocin (STZ) and cholesterol in rats. Materials and Methods: The animals were assigned to three groups, as follows: Group 1 (Control): buffer citrate solution 0.1 mL/100 g body; Group 2 (STZ): 20 mg/kg body STZ and fat diet (10 g cholesterol/100 g diet); Group 3 (STZ+Zn): 20 mg/kg body STZ + 5 mg/kg body Zn chloride and the same fat diet. DM was induced by administering STZ in a single take daily, for three consecutive days, Zn and citrate buffer were administered orally for a month. The protocol was approved by the Ethics Committee of the University ‘Grigore T Popa’ Iasi, in agreement with the International Regulations about the handling of laboratory animals. Results: The use of STZ in rats fed with cholesterol was correlated with important weight gain, hyperglycemia, the intensification of the transaminases activity and the increase in serum alkaline phosphatase, cholesterol, triglyceride, urea, creatinine and in malondialdehyde. Conclusions: The treatment with Zn resulted in weight loss and a decrease in blood sugar in diabetic rats. Supplementation with Zn notably reduced oxidative stress, preserved the pancreatic architecture and restored the liver and kidney function and structure in STZ-induced DM in rats.
In-Process Analysis of Melt Pool Fluctuations with Scanning Optical Coherence Tomography for Laser Welding of Copper for Quality Monitoring
Authors: Will Thomas , Jeron Tobias , Hoelbling Claudio , Müller Lars , Schmidt Michael
Published: November 2022

Optical coherence tomography (OCT) is an inline process monitoring technology for laser welding with various applications in the pre-, in-, and post-process. In-process monitoring with OCT focuses on the measurement of weld depth by the placement of a singular measurement beam into the keyhole. A laterally scanned measurement beam gives the opportunity to measure the keyhole and melt pool width. The processing region can be identified by separating higher signal intensities on the workpiece surface from lower signal intensities from the keyhole and the melt pool. In this work, we apply a scanned measurement beam for the identification of keyhole fluctuations. Different laser processing parameters are varied for laser welding of copper to evoke welds in the heat conduction regime, stable deep penetration welding, and unstable deep penetration welding. As keyhole instabilities can be related to the generation of spatter and other defects, we identified a feature for the classification of different weld statuses. In consequence, feedback can be given about possible defects which are originated in keyhole fluctuations (e.g., spatter).
Novel Supercapacitor Electrode Derived from One Dimensional Cerium Hydrogen Phosphate (1D-Ce(HPO4)2.xH2O)
Authors: Yoon Jong Hee , Jinsoo Bak , Cho Inho , Vinodh Rajangam , Pollet Bruno G. , Babu Rajendran Suresh , Kim Hee-Je , Kim Sungshin
Published: November 2022

In this manuscript, we are reporting for the first time one dimensional (1D) cerium hydrogen phosphate (Ce(HPO4)2.xH2O) electrode material for supercapacitor application. In short, a simple hydrothermal technique was employed to prepare Ce(HPO4)2.xH2O. The maximum surface area of 82 m2 g−1 was obtained from nitrogen sorption isotherm. SEM images revealed Ce(HPO4)2.xH2O exhibited a nanorod-like structure along with particles and clusters. The maximum specific capacitance of 114 F g−1 was achieved at 0.2 A g−1 current density for Ce(HPO4)/NF electrode material in a three-electrode configuration. Furthermore, the fabricated symmetric supercapacitor (SSC) based on Ce(HPO4)2.xH2O//Ce(HPO4)2.xH2O demonstrates reasonable specific energy (2.08 Wh kg−1), moderate specific power (499.88 W kg−1), and outstanding cyclic durability (retains 92.7% of its initial specific capacitance after 5000 GCD cycles).
Unexpected Room Temperature Ferromagnetism of a Ball-Milled Graphene Oxide—Melamine Mixture
Authors: Vasiliev Vladimir P. , Kabachkov Eugene N. , Kulikov Alexander V. , Manzhos Roman A. , Morozov Iurii G. , Shulga Yury M.
Published: November 2022

Nitrogen-doped carbon nanomaterial (NDCNM) was synthesized by grinding a mixture of graphene oxide and melamine in a planetary mill with both balls and milling chamber of zirconium dioxide. In the electron spin resonance spectrum of NDCNM, a broad signal with g = 2.08 was observed in addition to a narrow signal at g = 2.0034. In the study using a vibrating-sample magnetometer, the synthesized material is presumably a ferromagnet with a coercive force of 100 Oe. The specific magnetization at 10,000 Oe is approximately 0.020 and 0.055 emu/g at room temperature and liquid nitrogen temperature, respectively.
Camel Grass Phenolic Compounds: Targeting Inflammation and Neurologically Related Conditions
Authors: Lopes Graciliana , Gomes Elisabete , Barbosa Mariana , Bernardo João , Valentão Patrícia
Published: November 2022

Background: The use of plants for therapeutic purposes has been supported by growing scientific evidence. Methods: This work consisted of (i) characterizing the phenolic compounds present in both aqueous and hydroethanol (1:1, v/v) extracts of camel grass, by hyphenated liquid chromatographic techniques, (ii) evaluating their anti-inflammatory, antioxidant, and neuromodulation potential, through in vitro cell and cell-free models, and (iii) establishing a relationship between the chemical profiles of the extracts and their biological activities. Results: Several caffeic acid and flavonoid derivatives were determined in both extracts. The extracts displayed scavenging capacity against the physiologically relevant nitric oxide (•NO) and superoxide anion (O2•−) radicals, significantly reduced NO production in lipopolysaccharide (LPS)-stimulated macrophages (RAW 264.7), and inhibited the activity of hyaluronidase (HAase), acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Some of these bioactivities were found to be related with the chemical profile of the extracts, namely with 3-caffeoylquinic, 4-caffeoylquinic, chlorogenic, and p-coumaric acids, as well as with luteolin and apigenin derivatives. Conclusions: This study reports, for the first time, the potential medicinal properties of aqueous and hydroethanol extracts of camel grass in the RAW 264.7 cell model of inflammation, and in neurologically related conditions.