bluuebunny/crossref_metadata_2025 huggingface dataset:
Achieving Integrated Daylighting and Electric Lighting Systems: Current State of the Art and Needed Research
Authors: Thomson Gregory D. , Davis Robert G. , Fernandes Luís , Wang Taoning
Published: June 2021

This paper presents the results of a multi-disciplinary effort to clarify the state of the art and the state of practice, and necessary future research for creating the seamless integration and application of light in buildings, regardless of source, which is purposely modulated to illuminate surfaces and designed in a way that is comfortable, healthy, pleasing, cost-effective, and energy efficient. The authors unwrap the research, tools, and technical gaps preventing the full integration of electric lighting and daylighting with advanced façades through the coordination of lighting and windows research activities. The study and a stakeholder workshop captured current technology readiness levels (TRL), as well as research thrusts and implementation guidelines, and identified research priorities, presenting an analysis of the current landscape of lighting metrics—and which metrics are in the critical path for developing integrated daylighting and electric lighting systems, and their design, installation, and technology guidelines. In addition, the study defined stakeholder coordination, pathways to interoperable technology, and the value of viewing the work of the individual research areas holistically rather than in isolation.
Optimum Thickness of Thermal Insulation with Both Economic and Ecological Costs of Heating and Cooling
Authors: Dylewski Robert , Adamczyk Janusz
Published: June 2021

The energy efficiency of the construction sector should be determined by the cleanliness of the environment and, thus, the health of society. The scientific aim of this article was to develop a methodology for determining the optimum thickness of thermal insulation, taking into account both economic and ecological aspects and considering both heating and cooling costs. The method takes into account the number of degree days of the heating period, as well as the number of degree days of the cooling period. Variants in terms of different types of thermal insulation, various types of construction materials for building walls, climatic zones and heat sources, were taken into consideration. In order to find the optimum thicknesses of thermal insulation, both in economic and ecological terms, a metacriterion was used. The optimum thicknesses of thermal insulation with the use of the metacriterion were obtained in the range of 0.11–0.55 m. It was observed that the values of the optimum heat transfer coefficients for economic and ecological reasons do not depend on the type of construction materials used for vertical walls. The type of applied heat source is of the greatest importance for the size of the economic and ecological benefits. The proposed mathematical model for determining the optimum thickness of thermal insulation with the use of a metacriterion is a kind of generalization of earlier models from the literature.
Sinusoidal Control of a Brushless DC Motor with Misalignment of Hall Sensors
Authors: Kolano Krzysztof , Drzymała Bartosz , Gęca Jakub
Published: June 2021

This article presents an estimation method of the BLDC rotor position with asymmetrically arranged Hall sensors. Position estimation is necessary to control the motor by methods other than block commutation. A sinusoidal control method was selected for the research, which significantly reduces torque ripples and acoustic noise and is quite simple to implement. Inaccurate performance of the elements determining the position of the BLDC motor rotor causes a large error in the position estimation and has a negative impact on the operation of the drive controlled in this way. Using the developed control algorithms, it is possible to correctly determine the mechanical position of the rotor even for multi-pole motors. The proposed method is relatively easy to implement and does not require modification of control systems, being limited to changes only in the software of such devices. The tests of the actual system clearly show the usefulness of such a control method and its effectiveness.
Foraging Site Selection of the Middle Spotted Woodpecker (Leiopicus medius L.) in Primeval Oak-Lime-Hornbeam Forest of the Białowieża National Park: Comparison of Breeding and Non-Breeding Seasons
Authors: Stański Tomasz , Stańska Marzena , Goławski Artur , Czeszczewik Dorota
Published: June 2021

The distribution of the Middle Spotted Woodpecker (Leiopicus medius) is restricted to mature deciduous forests with large trees, mainly oaks (Quercus spp.). Intensive forest management resulted in the loss of many suitable habitats, thus resulting in a decline in the population of this species. This study aimed to identify the parameters of foraging sites in the breeding season (April to June) and in the non-breeding season (other months). The research was conducted in the primeval oak-lime-hornbeam forest of the Białowieża National Park, where foraging woodpeckers were observed and detailed parameters of foraging sites were recorded. During the breeding season woodpeckers foraged primarily on European hornbeams (Carpinus betulus L.), but in non-breeding season the use of this tree species decreased by a factor of two, whereas the use of Norway spruces (Picea abies L.) increased more than twice. The most preferred tree species as a foraging site in both seasons was pedunculate oak (Quercus robur L.). In the non-breeding season, woodpeckers foraged at sites located higher, and the foraging session was longer compared with the breeding season. In both seasons, woodpeckers preferred dead and large trees and prey gleaning from the tree surface was their dominant foraging technique. Our results confirmed the key role of oaks and large trees, but also revealed the importance of European hornbeams and Norway spruces as foraging sites for the Middle Spotted Woodpecker.
Evaluation of Soil Organic Layers Thickness and Soil Organic Carbon Stock in Hemiboreal Forests in Latvia
Authors: Bārdule Arta , Butlers Aldis , Lazdiņš Andis , Līcīte Ieva , Zvirbulis Uldis , Putniņš Raimonds , Jansons Aigars , Adamovičs Andis , Razma Ģirts
Published: June 2021

In the forest land of many European countries, including hemiboreal Latvia, organic soils are considered to be large sources of greenhouse gas (GHG) emissions. At the same time, growing efforts are expected in the near future to decrease emissions from the Land Use, Land Use Change and Forestry sector, including lands with organic soils to achieve enhanced contributions to the emissions and removals balance target set by the Paris Agreement. This paper aims to describe the distribution of organic soil layer thickness in forest land based on national forest inventory data and to evaluate soil organic carbon stock in Latvian forests classified as land with organic soil. The average thickness of the forest floor (organic material consisting of undecomposed or partially decomposed litter, O horizon) was greatest in coniferous forests with wet mineral soil, and decreased with increasing soil fertility. However, forest stand characteristics, including basal area and age, were weak predictors of O horizon thickness. In forests with organic soil, a lower proportion of soil organic matter layer (H horizon) in the top 70 cm soil layer, but a higher soil organic carbon stock both in the 0–30 cm layer and in the 0–100 cm layer was found in drained organic soils than in wet organic soils. Furthermore, the distribution of the soil H horizon thickness across different forest site types highlighted the potential overestimation of area of drained organic soils in Latvian forest land reported within the National GHG Inventory.
BARD1 Autoantibody Blood Test for Early Detection of Ovarian Cancer
Authors: Pilyugin Maxim , Ratajska Magdalena , Stukan Maciej , Concin Nicole , Zeillinger Robert , Irminger-Finger Irmgard
Published: June 2021

Background: Ovarian cancer (OC) is the most lethal gynaecological cancer. It is often diagnosed at an advanced stage with poor chances for successful treatment. An accurate blood test for the early detection of OC could reduce the mortality of this disease. Methods: Autoantibody reactivity to 20 epitopes of BARD1 and concentration of cancer antigen 125 (CA125) were assessed in 480 serum samples of OC patients and healthy controls. Autoantibody reactivity and CA125 were also tested for 261 plasma samples of OC with or without mutations in BRCA1/2, BARD1, or other predisposing genes, and healthy controls. Lasso statistic regression was applied to measurements to develop an algorithm for discrimination between OC and controls. Findings and interpretation: Measurement of autoantibody binding to a number of BARD1 epitopes combined with CA125 could distinguish OC from healthy controls with high accuracy. This BARD1-CA125 test was more accurate than measurements of BARD1 autoantibody or CA125 alone for all OC stages and menopausal status. A BARD1-CA125-based test is expected to work equally well for average-risk women and high-risk women with hereditary breast and ovarian cancer syndrome (HBOC). Although these results are promising, further data on well-characterised clinical samples shall be used to confirm the potential of the BARD1-CA125 test for ovarian cancer screening.
Direct Measurement of B Lymphocyte Gene Expression Biomarkers in Peripheral Blood Transcriptomics Enables Early Prediction of Vaccine Seroconversion
Authors: Huang Dan , Liu Alex Y. N. , Leung Kwong-Sak , Tang Nelson L. S.
Published: June 2021

Peripheral blood transcriptome is a highly promising area for biomarker development. However, transcript abundances (TA) in these cell mixture samples are confounded by proportions of the component leukocyte subpopulations. This poses a challenge to clinical applications, as the cell of origin of any change in TA is not known without prior cell separation procedure. We developed a framework to develop a cell-type informative TA biomarkers which enable determination of TA of a single cell-type (B lymphocytes) directly in cell mixture samples of peripheral blood (e.g., peripheral blood mononuclear cells, PBMC) without the need for subpopulation separation. It is applicable to a panel of genes called B cell informative genes. Then a ratio of two B cell informative genes (a target gene and a stably expressed reference gene) obtained in PBMC was used as a new biomarker to represent the target gene expression in purified B lymphocytes. This approach, which eliminates the tedious procedure of cell separation and directly determines TA of a leukocyte subpopulation in peripheral blood samples, is called the Direct LS-TA method. This method is applied to gene expression datasets collected in influenza vaccination trials as early predictive biomarkers of seroconversion. By using TNFRSF17 or TXNDC5 as the target genes and TNFRSF13C or FCRLA as the reference genes, the Direct LS-TA B cell biomarkers were determined directly in the PBMC transcriptome data and were highly correlated with TA of the corresponding target genes in purified B lymphocytes. Vaccination responders had almost a 2-fold higher Direct LS-TA biomarker level of TNFRSF17 (log 2 SMD = 0.84, 95% CI = 0.47–1.21) on day 7 after vaccination. The sensitivity of these Direct LS-TA biomarkers in the prediction of seroconversion was greater than 0.7 and area-under curves (AUC) were over 0.8 in many datasets. In this paper, we report a straightforward approach to directly estimate B lymphocyte gene expression in PBMC, which could be used in a routine clinical setting. Moreover, the method enables the practice of precision medicine in the prediction of vaccination response. More importantly, seroconversion could now be predicted as early as day 7. As the acquired immunology pathway is common to vaccination against influenza and COVID-19, these biomarkers could also be useful to predict seroconversion for the new COVID-19 vaccines.
Parental Mental Well-Being and Frequency of Adult-Child Nature Visits: The Mediating Roles of Parents’ Perceived Barriers
Authors: Gustafsson Jasmine , Ojala Ann , Hiltunen Pauliina , Engberg Elina , Wiklund-Engblom Annika , Törnwall Nea , Roos Eva , Ray Carola
Published: June 2021

Regular access to green space has been shown to provide several health benefits for children. However, children today spend less time outdoors. Thus, it has become important to understand what drives and limits children’s activities in nature. Based on a Finnish online survey of 1463 parents of children aged 2–7 conducted in 2019, the current study examined parents’ perceived barriers to visiting nature with their children. It also examined how parental mental well-being is related to families’ frequency of nature visits, and whether this association is mediated by different categories of parents’ perceived barriers. Eleven out of 12 barriers were largely perceived by parents as reasons that did not prevent them from visiting nature with their children. Next, factor analysis indicated a three-factor solution to the barriers. The results of a multiple mediation analysis showed that better parental mental well-being was associated with more frequent adult-child nature visits, and this relationship was partially mediated by a “lack of competence and logistics” and a “lack of time and interest”, but not by “insecurity and fear”. The results indicated that parents with poor mental well-being were more likely to perceive barriers to visiting nature, which in turn appeared to be related to a higher likelihood of having children who visited nature less frequently.
The Effect of Periodontitis on Dementia and Cognitive Impairment: A Meta-Analysis
Authors: Guo Haiying , Chang Shuli , Pi Xiaoqin , Hua Fang , Jiang Han , Liu Chang , Du Minquan
Published: June 2021

The association between periodontal disease and dementia/cognitive impairment continues to receive increasing attention. However, whether periodontal disease is a risk factor for dementia/cognitive impairment is still uncertain. This meta-analysis was conducted to comprehensively analyze the effect of periodontitis on dementia and cognitive impairment, and to assess the periodontal status of dementia patients at the same time. A literature search was undertaken on 19 October 2020 using PubMed, Web of Science, and Embase with different search terms. Two evaluators screened studies according to inclusion and exclusion criteria, and a third evaluator was involved if there were disagreements; this process was the same as that used for data extraction. Included studies were assessed with the Newcastle-Ottawa Scale (NOS), and results were analyzed using software Review Manager 5.2. Twenty observational studies were included. In the comparison between periodontitis and cognitive impairment, the odds ratio (OR) was 1.77 (95% confidence interval (CI), 1.31–2.38), which indicated that there was a strong relationship between periodontitis and cognitive impairment. There was no statistical significance in the effect of periodontitis on dementia (OR = 1.59; 95%CI, 0.92–2.76). The subgroup analysis revealed that moderate or severe periodontitis was significantly associated with dementia (OR = 2.13; 95%CI, 1.25–3.64). The mean difference (MD) of the community periodontal index (CPI) and clinical attachment level (CAL) was 0.25 (95%CI, 0.09–0.40) and 1.22 (95%CI, 0.61–1.83), respectively. In this meta-analysis, there was an association between periodontitis and cognitive impairment, and moderate or severe periodontitis was a risk factor for dementia. Additionally, the deterioration of periodontal status was observed among dementia patients.
Assessing Adolescent Dating Violence in the YourLife Project: Proposal of an Instrument for Spanish-Speaking Countries
Authors: Lopez-del Burgo Cristina , Osorio Alfonso , de la Rosa Pedro-Antonio , Calatrava María , de Irala Jokin
Published: June 2021

Background: Several instruments have been developed to assess adolescent dating violence but only few have been validated in Spanish-speaking settings. Some instruments are too long and may not be feasible to include them in a multipurpose questionnaire. We developed an instrument to be used in the YourLife project, an international project about young people lifestyles. Objective: We aimed to analyze the psychometric properties of this instrument in three Spanish-speaking countries (Chile, Ecuador, and Spain). Method: We included 1049 participants, aged 13–18 years. Exploratory and confirmatory factor analyses were conducted. Associations between dating violence and variables expected to covariate with it (substance use, school peer aggression, justification of dating violence, and relationship power imbalance), were tested. Results: Two different constructs (psychological and physical/sexual) for suffered and perpetrated violence were identified and confirmed in the three countries. The dating violence subscales had Cronbach’s alpha scores higher than 0.85. The strongest associations between dating violence and variables related to it were found within the relationship power imbalance items, suggesting that these items may be useful to detect adolescent dating violence when a specific questionnaire cannot be implemented. Conclusion: This instrument seems to be adequate to assess suffered and perpetrated adolescent dating violence within a multipurpose questionnaire among schooled adolescents.
A Chinese Version of the Caring Dimensions Inventory: Reliability and Validity Assessment
Authors: Tong Lai-Kun , Zhu Ming-Xia , Wang Si-Chen , Cheong Pak-Leng , Van Iat-Kio
Published: June 2021

Caring is central to nursing practice. Chinese nurses take care for the health of about 20% of the world’s population and their perception of caring is critical. However, until recently, instrument specifically designed to measure the caring of nurses in China was not found. Caring dimensions inventory (CDI) is one of the most frequently used instrument when measuring caring and is applicable to nurses from different cultures. The aim of this study is to test the validity and reliability of the Chinese version of the 25-item CDI. The English version of CDI was translated into Chinese according to the guidelines of the World Health Organization. Content validity was conducted among seven senior nurses from different institutions in different cities in China. A convenience sample of nurses from 11 cities in China was employed. Exploratory factor analysis and confirmatory factor analysis of the CDI was carried out using a sample of 880 nurses. The overall content validity index was 0.98. Three factors (Nurturance, Collaboration, Skill) were identified in exploratory factor analysis and were confirmed by confirmatory factor analysis. The three-factor solution explains 70.15% of the total variance. The Cronbach alpha for overall the CDI was 0.97. This study demonstrated that the Chinese version of the CDI showed satisfactory reliability and validity, indicating that it could be a useful measurement to assess nurses’ perception of caring in China.
Assessing Cognitive Abilities Using the WAIS-IV: An Item Response Theory Approach
Authors: Abdelhamid Gomaa Said Mohamed , Bassiouni Marwa Gomaa Abdelghani , Gómez-Benito Juana
Published: June 2021

Background: The Wechsler Adult Intelligence Scale-Fourth Edition (WAIS-IV) has been adapted to 28 different cultures and there has been considerable interest in examining its structure through exploratory and confirmatory factor analysis. This study investigates item and scale properties of the Egyptian WAIS-IV using item response theory (IRT) models. Methods: The sample consisted of 250 adults from Egypt. The item-level and subtest statistical properties of the Egyptian WAIS-IV were established using a combination of four dichotomous IRT models and four polytomous IRT models. In addition, factor analysis was performed to investigate the dimensionality of each subtest. Results: Factor analysis indicated the unidimensionality of each subtest. Among IRT models, the two-parameter logistic model provided a good fit for dichotomous subtests, while the graded response model fitted the polytomous data. Most items of the Egyptian WAIS-IV showed high discrimination, and the scale was adequately informative across the levels of latent traits (i.e., cognitive variables). However, each subtest included at least some items with limited ability to distinguish between individuals with differing levels of the cognitive variable being measured. Furthermore, most subtests have items that do not follow the difficulty rank they are ascribed in the WAIS-IV manual. Conclusions: Overall, the results suggest that the Egyptian WAIS-IV offers a highly valid assessment of intellectual abilities, despite the need for some improvements.
Stroking Rates of Open Water Swimmers during the 2019 FINA World Swimming Championships
Authors: Rodríguez Luis , Veiga Santiago , García Iker , González-Ravé José M
Published: June 2021

The aim of the present research was to examine the stroking rate (SR) values of successful and non-successful swimmers in the 10 km and 25 km races of the FINA 2019 World Swimming Championships. Data from 175 participants (95 men and 80 female) were classified according to their finishing positions. There were no meaningful differences in the overall SR values displayed by successful or non-successful participants during the 10 km and 25 km open water races of the FINA 2019 World Swimming Championships. However, there were changes in the SR throughout the races that depended on the swimmer’s performance group and gender. Successful swimmers in the 10 km event typically displayed even SR in the first 5 km but, unlike the remaining performance groups, increased their SR at some point in the second 5 km of the race. In the 25 km race, successful female swimmers presented an even SR profile for most of the race, whereas successful males presented a more variable profile. Nevertheless, no relationships between partial or average SR and finishing positions occurred, either in the 10 km or in the 25 km race. Changes in the SR values should be included in the race plan of open water swimmers according to tactical and pacing strategies.
CEO Turnovers: Transparency of Announcements and the Outperformance Puzzle
Authors: Farah Paul , Li Hui
Published: June 2021

This study investigates market reactions to announcements of CEO turnover and finds that forced turnovers are not accompanied by positive returns, which contradicts the broad view that firing a CEO sends a positive signal to the market. This contradiction is further explored by focusing on the nature of not only turnover but also a firm’s past performance. This study finds that the market seems to incorporate both types of information in reacting to CEO turnover announcements. Firing an underperforming CEO is viewed as a positive signal, whereas firing an outperforming CEO is viewed as a negative signal. Rather than taking early action against CEOs for a deterioration in their performance, firms appear to be firing outperforming CEOs owing to their apparent nonperformance-related reasons. This study also explores reasons behind the decision to fire a CEO from different news databases and finds that giving no clear reasons for a CEO’s departure increases uncertainty in the market, thereby causing a negative market reaction. However, stating performance as the reason for the departure assures investors about the future trajectory of the firm and results in a positive market reaction.
A Closed-Form Solution to Planar Feature-Based Registration of LiDAR Point Clouds
Authors: Wang Yongbo , Zheng Nanshan , Bian Zhengfu
Published: June 2021

Since pairwise registration is a necessary step for the seamless fusion of point clouds from neighboring stations, a closed-form solution to planar feature-based registration of LiDAR (Light Detection and Ranging) point clouds is proposed in this paper. Based on the Plücker coordinate-based representation of linear features in three-dimensional space, a quad tuple-based representation of planar features is introduced, which makes it possible to directly determine the difference between any two planar features. Dual quaternions are employed to represent spatial transformation and operations between dual quaternions and the quad tuple-based representation of planar features are given, with which an error norm is constructed. Based on L2-norm-minimization, detailed derivations of the proposed solution are explained step by step. Two experiments were designed in which simulated data and real data were both used to verify the correctness and the feasibility of the proposed solution. With the simulated data, the calculated registration results were consistent with the pre-established parameters, which verifies the correctness of the presented solution. With the real data, the calculated registration results were consistent with the results calculated by iterative methods. Conclusions can be drawn from the two experiments: (1) The proposed solution does not require any initial estimates of the unknown parameters in advance, which assures the stability and robustness of the solution; (2) Using dual quaternions to represent spatial transformation greatly reduces the additional constraints in the estimation process.
Leveraging Road Characteristics and Contributor Behaviour for Assessing Road Type Quality in OSM
Authors: Alghanim Amerah , Jilani Musfira , Bertolotto Michela , McArdle Gavin
Published: June 2021

Volunteered Geographic Information (VGI) is often collected by non-expert users. This raises concerns about the quality and veracity of such data. There has been much effort to understand and quantify the quality of VGI. Extrinsic measures which compare VGI to authoritative data sources such as National Mapping Agencies are common but the cost and slow update frequency of such data hinder the task. On the other hand, intrinsic measures which compare the data to heuristics or models built from the VGI data are becoming increasingly popular. Supervised machine learning techniques are particularly suitable for intrinsic measures of quality where they can infer and predict the properties of spatial data. In this article we are interested in assessing the quality of semantic information, such as the road type, associated with data in OpenStreetMap (OSM). We have developed a machine learning approach which utilises new intrinsic input features collected from the VGI dataset. Specifically, using our proposed novel approach we obtained an average classification accuracy of 84.12%. This result outperforms existing techniques on the same semantic inference task. The trustworthiness of the data used for developing and training machine learning models is important. To address this issue we have also developed a new measure for this using direct and indirect characteristics of OSM data such as its edit history along with an assessment of the users who contributed the data. An evaluation of the impact of data determined to be trustworthy within the machine learning model shows that the trusted data collected with the new approach improves the prediction accuracy of our machine learning technique. Specifically, our results demonstrate that the classification accuracy of our developed model is 87.75% when applied to a trusted dataset and 57.98% when applied to an untrusted dataset. Consequently, such results can be used to assess the quality of OSM and suggest improvements to the data set.
Research on HAR-Based Floor Positioning
Authors: Qi Hongxia , Wang Yunjia , Bi Jingxue , Cao Hongji , Xu Shenglei
Published: June 2021

Floor positioning is an important aspect of indoor positioning technology, which is closely related to location-based services (LBSs). Currently, floor positioning technologies are mainly based on radio signals and barometric pressure. The former are impacted by the multipath effect, rely on infrastructure support, and are limited by different spatial structures. For the latter, the air pressure changes with the temperature and humidity, the deployment cost of the reference station is high, and different terminal models need to be calibrated in advance. In view of these issues, here, we propose a novel floor positioning method based on human activity recognition (HAR), using smartphone built-in sensor data to classify pedestrian activities. We obtain the degree of the floor change according to the activity category of every step and determine whether the pedestrian completes floor switching through condition and threshold analysis. Then, we combine the previous floor or the high-precision initial floor with the floor change degree to calculate the pedestrians’ real-time floor position. A multi-floor office building was chosen as the experimental site and verified through the process of alternating multiple types of activities. The results show that the pedestrian floor position change recognition and location accuracy of this method were as high as 100%, and that this method has good robustness and high universality. It is more stable than methods based on wireless signals. Compared with one existing HAR-based method and air pressure, the method in this paper allows pedestrians to undertake long-term static or round-trip activities during the process of going up and down the stairs. In addition, the proposed method has good fault tolerance for the misjudgment of pedestrian actions.
A Cost Function for the Uncertainty of Matching Point Distribution on Image Registration
Authors: Bian Yuxia , Wang Meizhen , Chu Yongbin , Liu Zhihong , Chen Jun , Xia Zhiye , Fang Shuhong
Published: June 2021

Computing the homography matrix using the known matching points is a key step in computer vision for image registration. In practice, the number, accuracy, and distribution of the known matching points can affect the uncertainty of the homography matrix. This study mainly focuses on the effect of matching point distribution on image registration. First, horizontal dilution of precision (HDOP) is derived to measure the influence of the distribution of known points on fixed point position accuracy on the image. The quantization function, which is the average of the center points’ HDOP* of the overlapping region, is then constructed to measure the uncertainty of matching distribution. Finally, the experiments in the field of image registration are performed to verify the proposed function. We test the consistency of the relationship between the proposed function and the average of symmetric transfer errors. Consequently, the proposed function is appropriate for measuring the uncertainty of matching point distribution on image registration.
Regulation of Immune Functions by Non-Neuronal Acetylcholine (ACh) via Muscarinic and Nicotinic ACh Receptors
Authors: Mashimo Masato , Moriwaki Yasuhiro , Misawa Hidemi , Kawashima Koichiro , Fujii Takeshi
Published: June 2021

Acetylcholine (ACh) is the classical neurotransmitter in the cholinergic nervous system. However, ACh is now known to regulate various immune cell functions. In fact, T cells, B cells, and macrophages all express components of the cholinergic system, including ACh, muscarinic, and nicotinic ACh receptors (mAChRs and nAChRs), choline acetyltransferase, acetylcholinesterase, and choline transporters. In this review, we will discuss the actions of ACh in the immune system. We will first briefly describe the mechanisms by which ACh is stored in and released from immune cells. We will then address Ca2+ signaling pathways activated via mAChRs and nAChRs on T cells and B cells, highlighting the importance of ACh for the function of T cells, B cells, and macrophages, as well as its impact on innate and acquired (cellular and humoral) immunity. Lastly, we will discuss the effects of two peptide ligands, secreted lymphocyte antigen-6/urokinase-type plasminogen activator receptor-related peptide-1 (SLURP-1) and hippocampal cholinergic neurostimulating peptide (HCNP), on cholinergic activity in T cells. Overall, we stress the fact that ACh does not function only as a neurotransmitter; it impacts immunity by exerting diverse effects on immune cells via mAChRs and nAChRs.
Substantial Variations in the Optical Absorption and Reflectivity of Graphene When the Concentrations of Vacancies and Doping with Fluorine, Nitrogen, and Oxygen Change
Authors: Jiménez-González Ali Fransuani , Ramírez-de-Arellano Juan Manuel , Magaña Luis Fernando
Published: June 2021

We performed ab initio numerical simulations with the density functional theory to investigate the variations in the band structure, optical absorption, and the reflectivity of vacancy-graphene doped with nitrogen, oxygen, and fluorine for different densities. We considered the density values 0.78%, 1.02%, 1.39%, 2.00%, 3.12%, 5.55%, and 12.5% for the vacancies and doping. In the infrared and visible ranges for all cases, vacancies included, there is a substantial increment in the absorption and reflectivity concerning graphene. The most significant changes are for fluorine and oxygen at a concentration of 12.5%.
Recent Progress in Metabolic Syndrome Research and Therapeutics
Authors: Kao Ting-Wei , Huang Chin-Chou
Published: June 2021

Metabolic syndrome (MetS) is a well-defined yet difficult-to-manage disease entity. Both the precipitous rise in its incidence due to contemporary lifestyles and the growing heterogeneity among affected populations present unprecedented challenges. Moreover, the predisposed risk for developing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in populations with MetS, and the viral impacts on host metabolic parameters, underscores the need to investigate this mechanism thoroughly. Recent investigations of metabolomics and proteomics have revealed not only differentially expressed substances in MetS, but also the consequences of diet consumption and physical activity on energy metabolism. These variations in metabolites, as well as protein products, also influence a wide spectrum of host characteristics, from cellular behavior to phenotype. Research on the dysregulation of gut microbiota and the resultant inflammatory status has also contributed to our understanding of the underlying pathogenic mechanisms. As for state-of-the-art therapies, advancing depictions of the bio-molecular landscape of MetS have emerged and now play a key role in individualized precision medicine. Fecal microbiota transplantation, aiming to restore the host’s homeostasis, and targeting of the bile acid signaling pathway are two approaches to combatting MetS. Comprehensive molecular inquiries about MetS by omics measures are mandatory to facilitate the development of novel therapeutic modalities.
A Cardiovascular Risk Score for Use in Occupational Medicine
Authors: Affinito Giuseppina , Arpaia Pasquale , Barone-Adesi Francesco , Fontana Luca , Palladino Raffaele , Triassi Maria
Published: June 2021

Cardiovascular disease is one of the most frequent causes of long-term sickness absence from work. The study aims to develop and validate a score to assess the 10-year risk of unsuitability for work accounting for the cardiovascular risk. The score can be considered as a prevention tool that would improve the cardiovascular risk assessment during health surveillance visits under the assumption that a high cardiovascular risk might also translate into high risk of unsuitability for work. A total of 11,079 Italian workers were examined, as part of their scheduled occupational health surveillance. Cox proportional hazards regression models were employed to derive risk equations for assessing the 10-year risk of a diagnosis of unsuitability for work. Two scores were developed: the CROMA score (Cardiovascular Risk in Occupational Medicine) included age, sex, smoking status, blood pressure (systolic and diastolic), body mass index, height, diagnosis of hypertension, diabetes, ischemic heart disease, mental disorders and prescription of antidiabetic and antihypertensive medications. The CROMB score was the same as CROMA score except for the inclusion of only variables statistically significant at the 0.05 level. For both scores, the expected risk of unsuitability for work was higher for workers in the highest risk class, as compared with the lowest. Moreover results showed a positive association between most of cardiovascular risk factors and the risk of unsuitability for work. The CROMA score demonstrated better calibration than the CROMB score (11.624 (p-value: 0.235)). Moreover, the CROMA score, in comparison with existing CVD risk scores, showed the best goodness of fit and discrimination.
Adiponectin Associates with Rheumatoid Arthritis Risk in Overweight and Obesity Independently of Other Adipokines
Authors: Zhang Yuan , Johansson Linda , Andersson-Assarsson Johanna , Taube Magdalena , Peltonen Markku , Svensson Per-Arne , Herder Christian , Rudin Anna , Carlsson Lena , Rantapää-Dahlqvist Solbritt , Maglio Cristina
Published: June 2021

We recently reported that increased serum adiponectin was associated with rheumatoid arthritis (RA) risk in subjects with obesity. We hereby aim to determine if other adipokines associate with RA risk and if the association between adiponectin and RA is independent of other adipokines. Two nested-case control studies were performed in two different cohorts: 82 participants of the Swedish Obese Subjects (SOS) study who developed RA during follow-up matched with 410 controls, and 88 matched pairs from the Medical Biobank of Northern Sweden. Baseline levels of circulating adipokines were measured using ELISA. In a multivariable analysis in the SOS cohort, higher adiponectin was associated with an increased risk of RA independently of other adipokines (OR for RA risk: 1.06, 95% CI: 1.01–1.12, p = 0.02). No association between leptin, resistin, and visfatin levels and the risk of RA was detected. In the cohort from the Medical Biobank of Northern Sweden, higher adiponectin was associated with an increased risk of RA only in participants with overweight/obesity (OR: 1.17, 95% CI: 1.01−1.36, p = 0.03), independently of other adipokines. Our results show that in individuals with overweight/obesity, higher circulating levels of adiponectin, but not leptin, resistin, or visfatin, were associated with an increased RA risk.
Characterization of the Use of Emergency Contraception from Sentinel Pharmacies in a Region of Southern Europe
Authors: Jambrina Anna M. , Rius Pilar , Gascón Pilar , Armelles Mercè , Camps-Bossacoma Mariona , Franch Àngels , Rabanal Manel
Published: June 2021

Numerous studies have been published suggesting that emergency contraception (EC) is used repeatedly, but a lack of information regarding the profile of users makes it difficult to evaluate actual consumer habits. The aim of this study was to obtain information regarding the profile of users who obtain EC and other factors that might play a role, and to provide criteria to evaluate and improve the strategies of current contraceptive programs. This was an observational one-year study based on surveillance data on the provision of EC to women of reproductive age in 60 community pharmacies in Catalonia, Spain. In total, 941 notifications of dispensation of EC in Catalonia were received. A total of 44.2% of users said it was not the first time that they had taken the medication (repeat user). The percentage of users who used condoms was lower in repeat users compared to first-time users (56.7% vs. 64.4%, p < 0.05). A total of 25.7% of users stated that they did not use any barrier contraceptive method. The use of natural methods in repeat users was 53.8% in the subgroup who requested the medication after 48 h, significantly higher than in users who obtained the medication within the first 24 h (p < 0.05). A high percentage of repeat users with risky sexual behaviors were detected, suggesting that new measures must be implemented to provide information for this method, together with educational and preventive strategies.
Perioperative Perfusion of Allografts with Anti-Human T-lymphocyte Globulin Does Not Improve Outcome Post Liver Transplantation—A Randomized Placebo-Controlled Trial
Authors: Ritschl Paul Viktor , Günther Julia , Hofhansel Lena , Ernst Stefanie , Ebner Susanne , Sattler Arne , Weiß Sascha , Weissenbacher Annemarie , Oberhuber Rupert , Cardini Benno , Öllinger Robert , Biebl Matthias , Denecke Christian , Margreiter Christian , Resch Thomas , Schneeberger Stefan , Maglione Manuel , Kotsch Katja , Pratschke Johann
Published: June 2021

Due to the lack of suitable organs transplant surgeons have to accept unfavorable extended criteria donor (ECD) organs. Recently, we demonstrated that the perfusion of kidney organs with anti-human T-lymphocyte globulin (ATLG) prior to transplantation ameliorates ischemia-reperfusion injury (IRI). Here, we report on the results of perioperative ATLG perfusion in a randomized, single-blinded, placebo-controlled, feasibility trial (RCT) involving 30 liver recipients (LTx). Organs were randomly assigned for perfusion with ATLG/Grafalon® (AP) (n = 16) or saline only (control perfusion = CP) (n = 14) prior to implantation. The primary endpoint was defined as graft function reflected by aspartate transaminase (AST) values at day 7 post-transplantation (post-tx). With respect to the primary endpoint, no significant differences in AST levels were shown in the intervention group at day 7 (AP: 53.0 ± 21.3 mg/dL, CP: 59.7 ± 59.2 mg/dL, p = 0.686). Similarly, exploratory analysis of secondary clinical outcomes (e.g., patient survival) and treatment-specific adverse events revealed no differences between the study groups. Among liver transplant recipients, pre-operative organ perfusion with ATLG did not improve short-term outcomes, compared to those who received placebo perfusion. However, ATLG perfusion of liver grafts was proven to be a safe procedure without the occurrence of relevant adverse events.
Using Genealogical Concordance and Coalescent-Based Species Delimitation to Assess Species Boundaries in the Diaporthe eres Complex
Authors: Hilário Sandra , Gonçalves Micael F. M. , Alves Artur
Published: June 2021

DNA sequence analysis has been of the utmost importance to delimit species boundaries in the genus Diaporthe. However, the common practice of combining multiple genes, without applying the genealogical concordance criterion has complicated the robust delimitation of species, given that phylogenetic incongruence between loci has been disregarded. Despite the several attempts to delineate the species boundaries in the D. eres complex, the phylogenetic limits within this complex remain unclear. In order to bridge this gap, we employed the Genealogical Phylogenetic Species Recognition principle (GCPSR) and the coalescent-based model Poisson Tree Processes (PTPs) and evaluated the presence of recombination within the D. eres complex. Based on the GCPSR principle, presence of incongruence between individual gene genealogies, i.e., conflicting nodes and branches lacking phylogenetic support, was evident. Moreover, the results of the coalescent model identified D. eres complex as a single species, which was not consistent with the current large number of species within the complex recognized in phylogenetic analyses. The absence of reproductive isolation and barriers to gene flow as well as the high haplotype and low nucleotide diversity indices within the above-mentioned complex suggest that D. eres constitutes a population rather than different lineages. Therefore, we argue that a cohesive approach comprising genealogical concordance criteria and methods to detect recombination must be implemented in future studies to circumscribe species in the genus Diaporthe.
Changes in EEG Alpha Activity during Attention Control in Patients: Association with Sleep Disorders
Authors: Runnova Anastasiya , Selskii Anton , Kiselev Anton , Shamionov Rail , Parsamyan Ruzanna , Zhuravlev Maksim
Published: June 2021

We aimed to assess which quantitative EEG changes during daytime testing in patients with sleep disorder (primary insomnia and excessive daytime sleepiness groups). All experimental study participants were subjected to a long-term test for maintaining attention to sound stimuli, and their EEGs were recorded and then processed, using wavelet analysis, in order to estimate the power and frequency structure of alpha activity. In healthy subjects, the maximum increase in the alpha rhythm occurred near 9 Hz. Patients with primary insomnia were characterized by an increase in the amplitude of the alpha rhythm near 11 Hz. For subjects with sleep disorders, an increase in the amplitude of the alpha rhythm was observed in the entire frequency range (7.5–12.5 Hz), with a maximum increase at 9–10 Hz. Significant differences (p≤0.001) for changes in the alpha rhythm dynamics in the course of performing the attention test were observed in the frequency range of 7.5–10.5 Hz between the control group and patients with sleep disorders. The ratios of the alpha rhythm power values for passive stages with closed eyes before and after active stage were significantly different among the groups of healthy sleep volunteers, patients with primary insomnia, and patients with impaired sleep hygiene within the range of 9.5 to 12.5 Hz. The results of the current study supported the notion of a 24-h hyperarousal in primary insomnia.
Foreign-Language Phonetic Development Leads to First-Language Phonetic Drift: Plosive Consonants in Native Portuguese Speakers Learning English as a Foreign Language in Brazil
Authors: Osborne Denise M. , Simonet Miquel
Published: June 2021

Fifty-six Portuguese speakers born and raised in Brazil produced Portuguese words beginning in one of four plosives, /p b k ɡ/. Twenty-eight of them were monolinguals (controls), and the rest were learners of English as a foreign language (EFL). The learners were also asked to produce English words beginning with one of four plosives, /p b k ɡ/. We measured the plosives’ voice onset times (VOT) to address the following research questions: Do foreign-language learners, whose exposure to native English oral input is necessarily limited, form new sound categories specific to their additional language? Does engaging in the learning of a foreign language affect the phonetics of one’s native language? The EFL learners were found to differ from the controls in their production of Portuguese voiced (but not voiceless) plosives—prevoicing was longer in learner speech. The learners displayed different VOT targets for voiced (but not voiceless) consonants as a function of the language they were speaking—prevoicing was longer in Portuguese. In EFL learners’ productions, English sounds appear to be fundamentally modeled on phonologically similar native sounds, but some phonetic development (or reorganization) is found. Phonetic development induced by foreign-language learning may lead to a minor reconfiguration of the phonetics of native language sounds. EFL learners may find it challenging to learn the pronunciation patterns of English, likely due to the reduced access to native oral input.
Flavivirus: From Structure to Therapeutics Development
Authors: Zhao Rong , Wang Meiyue , Cao Jing , Shen Jing , Zhou Xin , Wang Deping , Cao Jimin
Published: June 2021

Flaviviruses are still a hidden threat to global human safety, as we are reminded by recent reports of dengue virus infections in Singapore and African-lineage-like Zika virus infections in Brazil. Therapeutic drugs or vaccines for flavivirus infections are in urgent need but are not well developed. The Flaviviridae family comprises a large group of enveloped viruses with a single-strand RNA genome of positive polarity. The genome of flavivirus encodes ten proteins, and each of them plays a different and important role in viral infection. In this review, we briefly summarized the major information of flavivirus and further introduced some strategies for the design and development of vaccines and anti-flavivirus compound drugs based on the structure of the viral proteins. There is no doubt that in the past few years, studies of antiviral drugs have achieved solid progress based on better understanding of the flavivirus biology. However, currently, there are no fully effective antiviral drugs or vaccines for most flaviviruses. We hope that this review may provide useful information for future development of anti-flavivirus drugs and vaccines.