bluuebunny/crossref_metadata_2025 huggingface dataset:
Paraneoplastic Dermatoses: A Brief General Review and an Extensive Analysis of Paraneoplastic Pemphigus and Paraneoplastic Dermatomyositis
Authors: Didona Dario , Fania Luca , Didona Biagio , Eming Rüdiger , Hertl Michael , Di Zenzo Giovanni
Published: March 2020

Skin manifestations of systemic disease and malignancy are extremely polymorphous. Clinicians should be familiarized with paraneoplastic dermatoses in order to perform an early diagnosis of the underlying neoplasm. Lack of familiarity with cutaneous clues of internal malignancy may delay diagnosis and treatment of cancer. In this review, we described several paraneoplastic dermatoses and discussed extensively two paradigmatic ones, namely paraneoplastic pemphigus and paraneoplastic dermatomyositis.
Ablation of Endothelial TRPV4 Channels Alters the Dynamic Ca2+ Signaling Profile in Mouse Carotid Arteries
Authors: McFarland Stuart J. , Weber David S. , Choi Chung-sik , Lin Mike T. , Taylor Mark S.
Published: March 2020

Transient receptor potential vanilloid 4 channels (TRPV4) are pivotal regulators of vascular homeostasis. Altered TRPV4 signaling has recently been implicated in various cardiovascular diseases, including hypertension and atherosclerosis. These versatile nonselective cation channels increase endothelial Ca2+ influx in response to various stimuli including shear stress and G protein-coupled receptor (GPCR) activation. Recent findings suggest TRPV4 channels produce localized Ca2+ transients at the endothelial cell plasma membrane that may allow targeted effector recruitment and promote large-scale Ca2+ events via release from internal stores (endoplasmic reticulum). However, the specific impact of TRPV4 channels on Ca2+ signaling in the intact arterial intima remains unknown. In the current study, we employ an endothelium-specific TRPV4 knockout mouse model (ecTRPV4−/−) to identify and characterize TRPV4-dependent endothelial Ca2+ dynamics. We find that carotid arteries from both ecTRPV4−/− and WT mice exhibit a range of basal and acetylcholine (ACh)-induced Ca2+ dynamics, similar in net frequency. Analysis of discrete Ca2+ event parameters (amplitude, duration, and spread) and event composite values reveals that while ecTRPV4−/− artery endothelium predominantly produces large Ca2+ events comparable to and in excess of those produced by WT endothelium, they are deficient in a particular population of small events, under both basal and ACh-stimulated conditions. These findings support the concept that TRPV4 channels are responsible for generating a distinct population of focal Ca2+ transients in the intact arterial endothelium, likely underlying their essential role in vascular homeostasis.
Therapeutic Potential of Volatile Terpenes and Terpenoids from Forests for Inflammatory Diseases
Authors: Kim Taejoon , Song Bokyeong , Cho Kyoung Sang , Lee Im-Soon
Published: March 2020

Forest trees are a major source of biogenic volatile organic compounds (BVOCs). Terpenes and terpenoids are known as the main BVOCs of forest aerosols. These compounds have been shown to display a broad range of biological activities in various human disease models, thus implying that forest aerosols containing these compounds may be related to beneficial effects of forest bathing. In this review, we surveyed studies analyzing BVOCs and selected the most abundant 23 terpenes and terpenoids emitted in forested areas of the Northern Hemisphere, which were reported to display anti-inflammatory activities. We categorized anti-inflammatory processes related to the functions of these compounds into six groups and summarized their molecular mechanisms of action. Finally, among the major 23 compounds, we examined the therapeutic potentials of 12 compounds known to be effective against respiratory inflammation, atopic dermatitis, arthritis, and neuroinflammation among various inflammatory diseases. In conclusion, the updated studies support the beneficial effects of forest aerosols and propose their potential use as chemopreventive and therapeutic agents for treating various inflammatory diseases.
Genome-Wide Identification and Analysis on YUCCA Gene Family in Isatis indigotica Fort. and IiYUCCA6-1 Functional Exploration
Authors: Qin Miaomiao , Wang Jing , Zhang Tianyi , Hu Xiangyang , Liu Rui , Gao Tian’e , Zhao Shuaijing , Yuan Yilin , Zheng Jinyu , Wang Zirong , Wei Xiying , Li Tao
Published: March 2020

Auxin is one of the most critical hormones in plants. YUCCA (Tryptophan aminotransferase of Arabidopsis (TAA)/YUCCA) enzymes catalyze the key rate-limiting step of the tryptophan-dependent auxin biosynthesis pathway, from IPA (Indole-3-pyruvateacid) to IAA (Indole-3-acetic acid). Here, 13 YUCCA family genes were identified from Isatis indigotica, which were divided into four categories, distributing randomly on chromosomes (2n = 14). The typical and conservative motifs, including the flavin adenine dinucleotide (FAD)-binding motif and flavin-containing monooxygenases (FMO)-identifying sequence, existed in the gene structures. IiYUCCA genes were expressed differently in different organs (roots, stems, leaves, buds, flowers, and siliques) and developmental periods (7, 21, 60, and 150 days after germination). Taking IiYUCCA6-1 as an example, the YUCCA genes functions were discussed. The results showed that IiYUCCA6-1 was sensitive to PEG (polyethylene glycol), cold, wounding, and NaCl treatments. The over-expressed tobacco plants exhibited high auxin performances, and some early auxin response genes (NbIAA8, NbIAA16, NbGH3.1, and NbGH3.6) were upregulated with increased IAA content. In the dark, the contents of total chlorophyll and hydrogen peroxide in the transgenic lines were significantly lower than in the control group, with NbSAG12 downregulated and some delayed leaf senescence characteristics, which delayed the senescence process to a certain extent. The findings provide comprehensive insight into the phylogenetic relationships, chromosomal distributions, and expression patterns and functions of the YUCCA gene family in I. indigotica.
An Intelligent Optimization Algorithm for Constructing a DNA Storage Code: NOL-HHO
Authors: Yin Qiang , Cao Ben , Li Xue , Wang Bin , Zhang Qiang , Wei Xiaopeng
Published: March 2020

The high density, large capacity, and long-term stability of DNA molecules make them an emerging storage medium that is especially suitable for the long-term storage of large datasets. The DNA sequences used in storage need to consider relevant constraints to avoid nonspecific hybridization reactions, such as the No-runlength constraint, GC-content, and the Hamming distance. In this work, a new nonlinear control parameter strategy and a random opposition-based learning strategy were used to improve the Harris hawks optimization algorithm (for the improved algorithm NOL-HHO) in order to prevent it from falling into local optima. Experimental testing was performed on 23 widely used benchmark functions, and the proposed algorithm was used to obtain better coding lower bounds for DNA storage. The results show that our algorithm can better maintain a smooth transition between exploration and exploitation and has stronger global exploration capabilities as compared with other algorithms. At the same time, the improvement of the lower bound directly affects the storage capacity and code rate, which promotes the further development of DNA storage technology.
Perspectives on the Potential Benefits of Antihypertensive Peptides towards Metabolic Syndrome
Authors: Jahandideh Forough , Wu Jianping
Published: March 2020

In addition to the regulation of blood pressure, the renin-angiotensin system (RAS) also plays a key role in the onset and development of insulin resistance, which is central to metabolic syndrome (MetS). Due to the interplay between RAS and insulin resistance, antihypertensive compounds may exert beneficial effects in the management of MetS. Food-derived bioactive peptides with RAS blocking properties can potentially improve adipose tissue dysfunction, glucose intolerance, and insulin resistance involved in the pathogenesis of MetS. This review discusses the pathophysiology of hypertension and the association between RAS and pathogenesis of the MetS. The effects of bioactive peptides with RAS modulating effects on other components of the MetS are discussed. While the in vivo reports on the effectiveness of antihypertensive peptides against MetS are encouraging, the exact mechanism by which these peptides infer their effects on glucose and lipid handling is mostly unknown. Therefore, careful design of experiments along with standardized physiological models to study the effect of antihypertensive peptides on insulin resistance and obesity could help to clarify this relationship.
Role of Interleukin-6 in Depressive Disorder
Authors: Ting Emily Yi-Chih , Yang Albert C. , Tsai Shih-Jen
Published: March 2020

Major depressive disorder (MDD), which is a leading psychiatric illness across the world, severely affects quality of life and causes an increased incidence of suicide. Evidence from animal as well as clinical studies have indicated that increased peripheral or central cytokine interleukin-6 (IL-6) levels play an important role in stress reaction and depressive disorder, especially physical disorders comorbid with depression. Increased release of IL-6 in MDD has been found to be a factor associated with MDD prognosis and therapeutic response, and may affect a wide range of depressive symptomatology. However, study results of the IL6 genetic effects in MDD are controversial. Increased IL-6 activity may cause depression through activation of hypothalamic-pituitary-adrenal axis or influence of the neurotransmitter metabolism. The important role of neuroinflammation in MDD pathogenesis has created a new perspective that the combining of blood IL-6 and other depression-related cytokine levels may help to classify MDD biological subtypes, which may allow physicians to identify the optimal treatment for MDD patients. To modulate the IL-6 activity by IL-6-related agents, current antidepressive agents, herb medication, pre-/probiotics or non-pharmacological interventions may hold great promise for the MDD patients with inflammatory features.
Optimization of the Emissions Profile of a Marine Propulsion System Using a Shaft Generator with Optimum Tracking-Based Control Scheme
Authors: Perez Joel R. , Reusser Carlos A.
Published: March 2020

Nowadays, marine propulsion systems based on thermal machines that operate under the diesel cycle have positioned themselves as one of the main options for this type of applications. The main comparative advantages of diesel engines, compared to other propulsion systems based on thermal cycle engines, are the low specific fuel consumption of residual fuels, and their higher thermal efficiency. However, its main disadvantage lies in the emissions produced by the combustion of the residual fuels, such as carbon dioxide (CO2), sulfur oxide (SOx), and nitrogen oxide (NOx). These emissions are directly related to the operating conditions of the propulsion system. Over the last decade, the International Maritime Organization (IMO) has adopted a series of regulations to reduce CO2 emissions based on the introduction of an Energy Efficiency Design Index (EEDI) and an Energy Efficiency Operational Indicator (EEOI). In this context, adding a Shaft Generator (SG) to the propulsion system favoring lower EEDI and EEOI values. The present work proposes a selective control system and optimization scheme that allows operating the shaft generator in Power Take Off (PTO) or Power Take In (PTI) mode, ensuring that the main engine operates, always, at the optimum fuel efficiency point, thus ensuring minimum CO2 emissions.
A Novel Framework of Real-Time Regional Collision Risk Prediction Based on the RNN Approach
Authors: Liu Dapei , Wang Xin , Cai Yao , Liu Zihao , Liu Zheng-Jiang
Published: March 2020

Regional collision risk identification and prediction is important for traffic surveillance in maritime transportation. This study proposes a framework of real-time prediction for regional collision risk by combining Density-Based Spatial Clustering of Applications with Noise (DBSCAN) technique, Shapley value method and Recurrent Neural Network (RNN). Firstly, the DBSCAN technique is applied to cluster vessels in specific sea area. Then the regional collision risk is quantified by calculating the contribution of each vessel and each cluster with Shapley value method. Afterwards, the optimized RNN method is employed to predict the regional collision risk of specific seas in short time. As a result, the framework is able to determine and forecast the regional collision risk precisely. At last, a case study is carried out with actual Automatic Identification System (AIS) data, the results show that the proposed framework is an effective tool for regional collision risk identification and prediction.
Investigation of Blade Tip Shape for Improving VAWT Performance
Authors: Jiang Yichen , He Chenlu , Zhao Peidong , Sun Tiezhi
Published: March 2020

Vertical axis wind turbine (VAWT) is a competitive power generation device due to structural simplicity, wind direction independence, no yaw mechanism required, easier maintenance, and lower noise emission. However, blade tip vortex will be generated at both ends of the blade during the rotation, resulting in torque loss and efficiency reduction. In this paper, computational fluid dynamics is used to study blade tip vortex and its reduction technique of a single-blade VAWT rotor in real scale. By monitoring the force and flow field at different heights of the blade, the influence ranges of tip vortex are obtained. The reduction effect of the bulkhead obtained from the blade profile curve is studied, and the size of the bulkhead is optimized. On the basis of adding the optimal bulkhead, the influence of the supporting strut is also explored. The joint action is obtained by changing the location of the supporting strut. The results show that the top supporting strut-bulkhead structure is the optimal position. The power-extraction efficiency of the rotor with this integrated structure is significantly improved at optimal tip speed ratios (TSRs) and higher TSRs.
Thermally Sprayed Coatings: Novel Surface Engineering Strategy Towards Icephobic Solutions
Authors: Koivuluoto Heli , Hartikainen Enni , Niemelä-Anttonen Henna
Published: March 2020

Surface engineering promotes possibilities to develop sustainable solutions to icing challenges. Durable icephobic solutions are under high interest because the functionality of many surfaces can be limited both over time and in icing conditions. To solve this, one potential approach is to use thermally sprayed polymer or composite coatings with multifunctional properties as a novel surface design method. In thermal spraying, coating materials and structures can be tailored in order to achieve different surface properties, e.g., wetting performance, roughness and protection against several weathering and wearing conditions. These, in turn, are beneficial for excellent icephobic performance and surface durability. The icephobicity of several different surfaces are tested in our icing wind tunnel (IWiT). Here, mixed-glaze ice is accreted from supercooled water droplets and the ice adhesion is measured using a centrifugal adhesion tester (CAT). The present study focuses on the icephobicity of thermally sprayed coatings. In addition, surface-related properties are evaluated in order to illustrate the correlation between the icephobic performance and the surface properties of differently tailored thermally sprayed coatings as well as compared those to other coatings and surfaces.
Experimental Investigations of Timber Beams with Stop-Splayed Scarf Carpentry Joints
Authors: Karolak Anna , Jasieńko Jerzy , Nowak Tomasz , Raszczuk Krzysztof
Published: March 2020

The paper presents the results of an experimental investigation of stop-splayed scarf joints, which was carried out as part of a research programme at the Wroclaw University of Science and Technology. A brief description and the characteristics of scarf and splice joints appearing in historical buildings are provided, with special reference to stop-splayed scarf joints (so-called ‘Bolt of lightning’) which were widely used, for example, in Italian renaissance architecture. Analyses and studies of scarf and splice joints in bent elements presented in the literature are reviewed, along with selected examples of analyses and research on tensile joints. It is worth noting that the authors in practically all the cited literature draw attention to the need for further research in this area. Next, the results of the authors’ own research on beams with stop-splayed scarf joints, strengthened using various methods, e.g., by means of drawbolts (metal screws), steel clamps and steel clamps with wooden pegs, which were subjected to four-point bending tests are presented. Load-deflection plots were obtained for load-bearing to bending of each beam in relation to the load-bearing of a continuous reference beam. A comparative analysis of the results obtained for each beam series is presented, along with conclusions and directions for further research.
Determination of Plate Corrosion Dimension Using Nd:YAG Pulsed Laser-generated Wavefield and Experimental Dispersion Curves
Authors: Tola Kassahun Demissie , Quoc Tran Dai , Yu Byoungjoon , Park Seunghee
Published: March 2020

Corrosion detection using a pulsed laser scanning system can be performed via ultrasonic wave propagation imaging. This method outputs illustrations of the wave field within the host structure; thus, it can depict wave–corrosion area interactions. Additionally, post-processing can be performed to enhance the visualization of corroded areas. The wavefield energy computed using RMS (Root Mean Square) is a validated post-processing tool capable of displaying the location and area of corrosion-damaged regions. Nonetheless, to characterize corrosion, it is necessary to determine its depth. The measurement of depth in conjunction with that of the corroded area via the RMS distribution enables the determination of all dimensions of corrosion damage. Thereafter, the flaw severity can be evaluated. This study employed a wavefield within a plate on which corrosion was developed artificially to generate frequency–wavenumber dispersion curves. The curves were compared with their counterparts from a corrosion-free plate. Alternatively, they could be compared with dispersion curves drawn using the depth and material properties of a pristine plate via a computer program. Frequency–wavenumber pairs were extracted from the dispersion curves produced using the portion of the wavefield within the corroded area. These were inserted into the Rayleigh–Lamb equation, from which depths were calculated and averaged.
Closed Cell Rigid Polyurethane Foams Based on Low Functionality Polyols: Research of Dimensional Stability and Standardised Performance Properties
Authors: Kairytė Agnė , Kremensas Arūnas , Balčiūnas Giedrius , Członka Sylwia , Strąkowska Anna
Published: March 2020

Currently, polyurethane foam producers come across the several problems when petroleum-based polyols are replaced with low functionality biomass, or waste-based, polyols. In addition, the dilemma is intensified with regulations that require full or partial replacement of blowing agents that can cause high ozone depletion with alternatives like water, which causes the formation of CO2. Therefore, these gases diffuse out of the foam so quickly that the polymeric cell walls cannot withstand the pressure, consequently causing huge dimensional changes at ambient temperature and humidity. Even though the theoretical stoichiometric balance is correct, the reality shows that it is not enough. Therefore, polyethylene terephthalate waste-based polyol was chosen as a low functionality polyol which was modified with high functionality sucrose-based polyol in order to obtain dimensionally stable polyurethane foams in the density range of 30–40 kg/m3. These more stable foams are characterized by linear changes no higher than 0.5%, short-term water absorption by partial immersion no higher than 0.35 kg/m2, and water vapor resistance factors up to 50. In order to obtain thermally efficient polyurethane foams, conventional blowing agents and water systems were implemented, thus, assuring thermal conductivity values in the range of 0.0198–0.0204 W/(m·K) and obtaining products which conform to all the requirements for performance of sprayed and factory-made polyurethane foam standards EN 14315-1 and EN 13165.
Enhancing the Surface Quality of Micro Titanium Alloy Specimen in WEDM Process by Adopting TGRA-Based Optimization
Authors: Thangaraj Muthuramalingam , Annamalai Ramamurthy , Moiduddin Khaja , Alkindi Mohammed , Ramalingam Sundar , Alghamdi Osama
Published: March 2020

The surface measures of machined titanium alloys as dental materials can be enhanced by adopting a decision-making algorithm in the machining process. The surface quality is normally characterized by more than one quality parameter. Hence, it is very important to establish multi-criteria decision making to compute the optimal process factors. In the present study, Taguchi–Grey analysis-based criteria decision making has been applied to the input process factors in the wire EDM (electric discharge machining) process. The recast layer thickness, wire wear ratio and micro hardness have been chosen to evaluate the quality measures. It was found that the wire electrode selection was the most influential factor on the quality measures in the WEDM process, due to its significance in creating spark energy. The optimal arrangement of the input process parameters has been found using the proposed approach as gap voltage (70 V), discharge current (15 A) and duty factor (0.6). It was proved that the proposed method can enhance the efficacy of the process. Utilizing the computed combination of optimal process parameters in surface quality analysis has significantly contributed to improving the quality of machining surface.
Microencapsulated Bio-Based Rejuvenators for the Self-Healing of Bituminous Materials
Authors: Norambuena-Contreras Jose , Arteaga-Perez Luis E. , Guadarrama-Lezama Andrea Y. , Briones Rodrigo , Vivanco Juan F. , Gonzalez-Torre Irene
Published: March 2020

Asphalt self-healing by encapsulated rejuvenating agents is considered a revolutionary technology for the autonomic crack-healing of aged asphalt pavements. This paper aims to explore the use of Bio-Oil (BO) obtained from liquefied agricultural biomass waste as a bio-based encapsulated rejuvenating agent for self-healing of bituminous materials. Novel BO capsules were synthesized using two simple dripping methods through dropping funnel and syringe pump devices, where the BO agent was microencapsulated by external ionic gelation in a biopolymer matrix of sodium alginate. Size, surface aspect, and elemental composition of the BO capsules were characterized by optical and scanning electron microscopy and energy-dispersive X-ray spectroscopy. Thermal stability and chemical properties of BO capsules and their components were assessed through thermogravimetric analysis (TGA-DTG) and Fourier-Transform Infrared spectroscopy (FTIR-ATR). The mechanical behavior of the capsules was evaluated by compressive and low-load micro-indentation tests. The self-healing efficiency over time of BO as a rejuvenating agent in cracked bitumen samples was quantified by fluorescence microscopy. Main results showed that the BO capsules presented an adequate morphology for the asphalt self-healing application, with good thermal stability and physical-chemical properties. It was also proven that the BO can diffuse in the bitumen reducing the viscosity and consequently self-healing the open microcracks.
Properties of Concrete with Recycled Concrete Aggregate Containing Metallurgical Sludge Waste
Authors: Pizoń Jan , Gołaszewski Jacek , Alwaeli Mohamed , Szwan Patryk
Published: March 2020

Sand has been considered to be something of an immeasurable quantity. There are many indications that this view is no longer valid and that the limiting of natural aggregates usage is doubly justified. Firstly, the extraction of natural aggregates is expensive and has a huge impact on the environment. The main issues in sand and gravel mining are the large areas that are affected, ground water level changes, illegal mining, unsuitability of desert and marine sand, and costs of transport. Secondly, metallurgical waste can be used as a substitute for natural aggregates. This is doubly beneficial—the waste is recycled and the use of natural aggregates is reduced. Waste is stored in landfills that take up large areas and there is also the possibility of ground and groundwater pollution by hazardous compounds. The research presented in this article focuses on the technological conditions of using metallurgical waste in its original form and as a component of recycled concrete aggregate (RCA). The use of metallurgical sludge waste or crushed or round RCA to produce concrete deteriorates the consistency and does not significantly affect the air content and density of the concrete mix. RCA lowers the density of hardened concrete. Metallurgical sludge waste or RCA usage adversely affect the absorbability and permeability of concrete. Concrete containing metallurgical sludge waste is of higher compressive strength after 7 and 28 days, with up to 60% of waste as a sand replacement. RCA concrete achieved higher compressive strength also.
Influence of Selective Laser Melting Technological Parameters on the Mechanical Properties of Additively Manufactured Elements Using 316L Austenitic Steel
Authors: Kluczyński Janusz , Śnieżek Lucjan , Grzelak Krzysztof , Janiszewski Jacek , Płatek Paweł , Torzewski Janusz , Szachogłuchowicz Ireneusz , Gocman Krzysztof
Published: March 2020

The main aim of this study was to investigate the influence of different energy density values used for the additively manufactured elements using selective laser melting (SLM).The group of process parameters considered was selected from the first-stage parameters identified in preliminary research. Samples manufactured using three different sets of parameter values were subjected to static tensile and compression tests. The samples were also subjected to dynamic Split–Hopkinson tests. To verify the microstructural changes after the dynamic tests, microstructural analyses were conducted. Additionally, the element deformation during the tensile tests was analyzed using digital image correlation (DIC). To analyze the influence of the selected parameters and verify the layered structure of the manufactured elements, sclerometer scratch hardness tests were carried out on each sample. Based on the research results, it was possible to observe the porosity growth mechanism and its influence on the material strength (including static and dynamic tests). Parameters modifications that caused 20% lower energy density, as well as elongation of the elements during tensile testing, decreased twice, which was strictly connected with porosity growth. An increase of energy density, by almost three times, caused a significant reduction of force fluctuations differences between both tested surfaces (parallel and perpendicular to the building platform) during sclerometer hardness testing. That kind of phenomenon had been taken into account in the microstructure investigations before and after dynamic testing, where it had been spotted as a positive impact on material deformations based on fused material formation after SLM processing.
Structural Characterization of the S-glycosylated Bacteriocin ASM1 from Lactobacillus plantarum
Authors: Goroncy Alexander K. , Loo Trevor S. , Koolaard Adrian M. , Patchett Mark L. , Norris Gillian E.
Published: March 2020

In order to protect their environmental niche, most bacteria secret antimicrobial substances designed to target specific bacterial strains that are often closely related to the producer strain. Bacteriocins, small, ribosomally synthesised antimicrobial peptides, comprise a class of such substances and can either inhibit (bacteriostatic) or kill (bactericidal) target cells. Glycocins are a class of bacteriocin that are post-translationally modified by one or more carbohydrate moieties that are either β-O-linked to either a serine or threonine and/or β-S-linked to a cysteine. The solution nuclear magnetic resonance structure (NMR) of the glycocin ASM1 (produced by Lactobacillus plantarum A-1), an orthologue of GccF, has been determined. In both structures, the disulfide bonds are essential for activity and restrict the mobility of the N-acetyl-glucosamine (GlcNAc) attached to Ser-18 (O-linked), compared to the much more flexible GlcNAc moiety on Cys-43 (S-linked). Interestingly, despite 88% sequence identity, the helical structure of ASM1 is less pronounced which appears to be consistent with the far ultra-violet circular dichroism (UV CD) spectra.
New Comparison Theorems for the Nth Order Neutral Differential Equations with Delay Inequalities
Authors: Moaaz Osama , Furuichi Shigeru , Muhib Ali
Published: March 2020

In this work, we present a new technique for the oscillatory properties of solutions of higher-order differential equations. We set new sufficient criteria for oscillation via comparison with higher-order differential inequalities. Moreover, we use the comparison with first-order differential equations. Finally, we provide an example to illustrate the importance of the results.
Marine Microalgae, Spirulina maxima-Derived Modified Pectin and Modified Pectin Nanoparticles Modulate the Gut Microbiota and Trigger Immune Responses in Mice
Authors: Chandrarathna H.P.S.U. , Liyanage T.D. , Edirisinghe S.L. , Dananjaya S.H.S. , Thulshan E.H.T. , Nikapitiya Chamilani , Oh Chulhong , Kang Do-Hyung , De Zoysa Mahanama
Published: March 2020

This study evaluated the modulation of gut microbiota, immune responses, and gut morphometry in C57BL/6 mice, upon oral administration of S. maxima-derived modified pectin (SmP, 7.5 mg/mL) and pectin nanoparticles (SmPNPs; 7.5 mg/mL). Metagenomics analysis was conducted using fecal samples, and mice duodenum and jejunum were used for analyzing the immune response and gut morphometry, respectively. The results of metagenomics analysis revealed that the abundance of Bacteroidetes in the gut increased in response to both modified SmP and SmPNPs (75%) as compared with that in the control group (66%), while that of Firmicutes decreased in (20%) as compared with that in the control group (30%). The mRNA levels of mucin, antimicrobial peptide, and antiviral and gut permeability-related genes in the duodenum were significantly (p < 0.05) upregulated (> 2-fold) upon modified SmP and SmPNPs feeding. Protein level of intestinal alkaline phosphatase was increased (1.9-fold) in the duodenum of modified SmPNPs feeding, evidenced by significantly increased goblet cell density (0.5 ± 0.03 cells/1000 µm2) and villi height (352 ± 10 µm). Our results suggest that both modified SmP and SmPNPs have the potential to modulate gut microbial community, enhance the expression of immune related genes, and improve gut morphology.
Effect of TiO2-Nanoparticles on Ni Electrodeposition on Copper Wire
Authors: Saad Samiha , Boumerzoug Zakaria , Helbert Anne Laure , Brisset François , Baudin Thierry
Published: March 2020

The objective of this work is to study, on a copper wire, the effect of TiO2-nanoparticles on electrodeposited nickel. Both the microstructure and surface morphology (texture) of the coating were investigated. This deposit is obtained from baths of sulfated electroplating Watts. The Ni-TO2 composite coating is deposited at a temperature of 45 °C. The composite deposit is prepared by adding nanoparticles of TiO2 to the electrolyte. The characterization has been carried out by X-ray diffraction, scanning electron microscopy, microhardness measurements, and electron backscatter diffraction (EBSD). Vickers microhardness was used to characterize the mechanical properties of the electrodeposited nickel. The results showed the effects of the TiO2 on the composition, the surface morphology, and the hardness of the deposited layer. However, there was not an effect of TiO2 nanoparticles on texture.
One-Step Femtosecond Laser Stealth Dicing of Quartz
Authors: Gaudiuso Caterina , Volpe Annalisa , Ancona Antonio
Published: March 2020

We report on a one-step method for cutting 250-µm-thick quartz plates using highly focused ultrashort laser pulses with a duration of 200 fs and a wavelength of 1030 nm. We show that the repetition rate, the scan speed, the pulse overlap and the pulse energy directly influence the cutting process and quality. Therefore, a suitable choice of these parameters was necessary to get single-pass stealth dicing with neat and flat cut edges. The mechanism behind the stealth dicing process was ascribed to tensile stresses generated by the relaxation of the compressive stresses originated in the laser beam focal volume during irradiation in the bulk material. Such stresses produced micro-fractures whose controlled propagation along the laser beam path led to cutting of the samples.
Potential PGPR Properties of Cellulolytic, Nitrogen-Fixing, Phosphate-Solubilizing Bacteria in Rehabilitated Tropical Forest Soil
Authors: Tang Amelia , Haruna Ahmed Osumanu , Majid Nik Muhamad Ab. , Jalloh Mohamadu Boyie
Published: March 2020

In the midst of the major soil degradation and erosion faced by tropical ecosystems, rehabilitated forests are being established to avoid the further deterioration of forest lands. In this context, cellulolytic, nitrogen-fixing (N-fixing), phosphate-solubilizing bacteria are very important functional groups in regulating the elemental cycle and plant nutrition, hence replenishing the nutrient content in forest soils. As is the case for other potential plant growth-promoting (PGP) rhizobacteria, these functional bacteria could have cross-functional abilities or beneficial traits that are essential for plants and can improve their growth. This study was conducted to isolate, identify, and characterize selected PGP properties of these three functional groups of bacteria from tropical rehabilitated forest soils at Universiti Putra Malaysia Bintulu Sarawak Campus, Malaysia. The bacteria were isolated based on their colonial growth on respective functional media, identified using both molecular and selected biochemical properties, and were assessed for their functional quantitative activities as well as PGP properties based on seed germination tests and indole-3-acetic acid (IAA) production. Out of the 15 identified bacterial isolates that exhibited beneficial phenotypic traits, a third belong to the genus Burkholderia and a fifth to Stenotrophomonas sp., with both genera consisting of members from two different functional groups. The results of the experiments confirm the multiple PGP traits of some selected bacterial isolates based on their respective high functional activities, root and shoot lengths, and seedling vigor improvements when bacterized on mung bean seeds, as well as significant IAA production. The results of this study suggest that these functional bacterial strains could potentially be included in bio-fertilizer formulations for crop growth on acid soils.
Prevalence, Antimicrobial Resistance, Virulence Genes and Genetic Diversity of Salmonella Isolated from Retail Duck Meat in Southern China
Authors: Chen Zhengquan , Bai Jie , Wang Shaojun , Zhang Xibin , Zhan Zeqiang , Shen Haiyan , Zhang Hongxia , Wen Junping , Gao Yuan , Liao Ming , Zhang Jianmin
Published: March 2020

Salmonella is an important cause of foodborne diseases. This study was undertaken to investigate the prevalence, serotype distribution, antimicrobial resistance, virulence genes, and genetic diversity of Salmonella isolates recovered from fresh duck meat obtained from retail markets in Southern China. In total, 365 samples of fresh duck meat were collected from retail markets in six different cities of Guangdong Province between May 2017 and April 2019. High levels of Salmonella contamination were detected in duck meat (151/365, 41.4%). Twenty-six different Salmonella serotypes were identified: S. Corvallis (n = 25, 16.6%), S. Kentucky (n = 22, 14.6%) and S. Agona (n = 20, 13.3%) were the most prevalent serotypes. All isolates were resistant to at least one antibiotic and 133 (88.1%) isolates exhibited multidrug resistance (MDR). Most (86.1%) Salmonella isolates carried seven classes of virulence-associated genes. This study showed the diversity of Salmonella serotypes and genotypes and the high prevalence of MDR isolates carrying multiple virulence-associated genes among isolates from duck meat obtained from retail markets in Southern China. Isolates from different districts had similar pulsed-field gel electrophoresis (PFGE) patterns indicating that circulating foodborne Salmonella constitutes a potential public health issue across different districts.
Evaluation of Cyprinid Herpesvirus 2 Latency and Reactivation in Carassius gibel
Authors: Chai Wenjun , Qi Lin , Zhang Yujun , Hong Mingming , Jin Ling , Li Lijuan , Yuan Junfa
Published: March 2020

Cyprinid herpesvirus 2 (CyHV-2, species Cyprinid herpesvirus 2) causes severe mortality in ornamental goldfish, crucian carp (Carassius auratus), and gibel carp (Carassius gibelio). It has been shown that the genomic DNA of CyHV-2 could be detected in subclinical fish, which implied that CyHV-2 could establish persistent infection. In this study, the latency of CyHV-2 was investigated in the survival fish after primary infection. CyHV-2 genomic DNA was detected in multiple tissues of acute infection samples; however, detection of CyHV-2 DNA was significantly reduced in fish recovered from the primary infection on day 300 postinfection. No active viral gene transcription, such as DNA polymerase and ORF99, was detected in recovered fish. Following temperature stress, an increase of CyHV-2 DNA copy numbers and gene transcription were observed in tissues examined, which suggests that CyHV-2 was reactivated under stress. In addition, a cell line (GCBLat1) derived from the brain tissue from CyHV-2-exposed fish harbored CyHV-2 genome but did not produce infectious virions under normal culture conditions. However, CyHV-2 replication and viral gene transcription occurred when GCBLat1 cells were treated with trichostatin A (TSA) or phorbol 12-myristate 13-acetate (TPA). It suggests CyHV-2 can remain latent in vitro and can reactivate under stress condition.
Identification and in vitro Characterization of a Novel Phage Endolysin that Targets Gram-Negative Bacteria
Authors: Bai Jaewoo , Lee Sangmi , Ryu Sangryeol
Published: March 2020

Most double-stranded (ds) DNA phages utilize holin proteins to secrete endolysin for host peptidoglycan lysis. In contrast, several holin-independent endolysins with secretion sequences or signal-arrest-release (SAR) sequences are secreted via the Sec pathway. In this study, we characterized a novel lysis protein (M4Lys) encoded by the dsDNA phage BSPM4, whose lysis function is not dependent on either holin or the Sec pathway in vitro. In silico analysis of M4Lys revealed that it contains a putative virion protein domain and an unusual C-terminal transmembrane domain (TMD). Turbidity reduction assays and liquid chromatography-mass spectrometry using purified peptidoglycan showed that the virion protein domain of M4Lys has peptidoglycan lysis activity. In vitro overproduction of M4Lys in Escherichia coli revealed that M4Lys alone caused rapid cell lysis. Treatment of E. coli with a Sec inhibitor did not inhibit the lysis activity of M4Lys, indicating that the Sec pathway is not involved in M4Lys-mediated cell lysis. Truncation of the TMD eliminated the cell lysis phenomenon, while production of the TMD alone did not induce the cell lysis. All these findings demonstrate that M4Lys is a novel endolysin that has a unique mosaic structure distinct from other canonical endolysins and the TMD plays a critical role in M4Lys-mediated in vitro cell lysis.
Whole Genome Sequencing Differentiates Presumptive Extended Spectrum Beta-Lactamase Producing Escherichia coli along Segments of the One Health Continuum
Authors: Adator Emelia H. , Walker Matthew , Narvaez-Bravo Claudia , Zaheer Rahat , Goji Noriko , Cook Shaun R. , Tymensen Lisa , Hannon Sherry J. , Church Deirdre , Booker Calvin W. , Amoako Kingsley , Nadon Celine A. , Read Ron , McAllister Tim A.
Published: March 2020

Antimicrobial resistance (AMR) has important implications for the continued use of antibiotics to control infectious diseases in both beef cattle and humans. AMR along the One Health continuum of the beef production system is largely unknown. Here, whole genomes of presumptive extended-spectrum β-lactamase E. coli (ESBL-EC) from cattle feces (n = 40), feedlot catch basins (n = 42), surrounding streams (n = 21), a beef processing plant (n = 4), municipal sewage (n = 30), and clinical patients (n = 25) are described. ESBL-EC were isolated from ceftriaxone selective plates and subcultured on ampicillin selective plates. Agreement of genotype-phenotype prediction of AMR ranged from 93.2% for ampicillin to 100% for neomycin, trimethoprim/sulfamethoxazole, and enrofloxacin resistance. Overall, β-lactam (100%; blaEC, blaTEM-1, blaSHV, blaOXA, blaCTX-M-), tetracycline (90.1%; tet(A), tet(B)) and folate synthesis (sul2) antimicrobial resistance genes (ARGs) were most prevalent. The ARGs tet(C), tet(M), tet(32), blaCTX-M-1, blaCTX-M-14, blaOXA-1, dfrA18, dfrA19, catB3, and catB4 were exclusive to human sources, while blaTEM-150, blaSHV-11–12, dfrA12, cmlA1, and cmlA5 were exclusive to beef cattle sources. Frequently encountered virulence factors across all sources included adhesion and type II and III secretion systems, while IncFIB(AP001918) and IncFII plasmids were also common. Specificity and prevalence of ARGs between cattle-sourced and human-sourced presumptive ESBL-EC likely reflect differences in antimicrobial use in cattle and humans. Comparative genomics revealed phylogenetically distinct clusters for isolates from human vs. cattle sources, implying that human infections caused by ESBL-EC in this region might not originate from beef production sources.
Controlled Sputtering Pressure on High-Quality Sb2Se3 Thin Film for Substrate Configurated Solar Cells
Authors: Tang Rong , Chen Xingye , Luo Yandi , Chen Zihang , Liu Yike , Li Yingfen , Su Zhenghua , Zhang Xianghua , Fan Ping , Liang Guangxing
Published: March 2020

Magnetron sputtering has become an effective method in Sb2Se3 thin film photovoltaic. Research found that post-selenization treatments are essential to produce stoichiometric thin films with desired crystallinity and orientation for the sputtered Sb2Se3. However, the influence of the sputtering process on Sb2Se3 device performance has rarely been explored. In this work, the working pressure effect was thoroughly studied for the sputtered Sb2Se3 thin film solar cells. High-quality Sb2Se3 thin film was obtained when a bilayer structure was applied by sputtering the film at a high (1.5 Pa) and a low working pressure (1.0 Pa) subsequently. Such bilayer structure was found to be beneficial for both crystallization and preferred orientation of the Sb2Se3 thin film. Lastly, an interesting power conversion efficiency (PCE) of 5.5% was obtained for the champion device.
Phosphorylation-Dependent SERS Readout for Activity Assay of Protein Kinase A in Cell Extracts
Authors: Liu Renyong , Xie Chenggen , Yan Yehan , Hu Lin , Wang Suhua , Alamry Khalid A. , Marwani Hadi M. , Chen Lijuan
Published: March 2020

Protein kinases are key regulators of cell function, the abnormal activity of which may induce several human diseases, including cancers. Therefore, it is of great significance to develop a sensitive and reliable method for assaying protein kinase activities in real biological samples. Here, we report the phosphorylation-dependent surface-enhanced Raman scattering (SERS) readout of spermine-functionalized silver nanoparticles (AgNPs) for protein kinase A (PKA) activity assay in cell extracts. In this assay, the presence of PKA would phosphorylate and alter the net charge states of Raman dye-labeled substrate peptides, and the resulting anionic products could absorb onto the AgNPs with cationic surface charge through electrostatic attraction. Meanwhile, the Raman signals of dyes labeled on peptides were strongly enhanced by the aggregated AgNPs with interparticle hot spots formed in assay buffer. The SERS readout was directly proportional to the PKA activity in a wide range of 0.0001–0.5 U·μL−1 with a detection limit as low as 0.00003 U·μL−1. Moreover, the proposed SERS-based assay for the PKA activity was successfully applied to monitoring the activity and inhibition of PKA in real biological samples, particularly in cell extracts, which would be beneficial for kinase-related disease diagnostics and inhibitor screening.
Removal and Recovery of Methyl Tertiary Butyl Ether (MTBE) from Water Using Carbon Nanotube and Graphene Oxide Immobilized Membranes
Authors: Intrchom Worawit , Roy Sagar , Mitra Somenath
Published: March 2020

Methyl tert-butyl ether (MTBE) is a widely used gasoline additive that has high water solubility, and is difficult to separate from contaminated ground and surface waters. We present the development in functionalized carbon nanotube-immobilized membranes (CNIM-f) and graphene oxide-immobilized membranes (GOIM) for enhanced separation of MTBE via sweep gas membrane distillation (SGMD). Both types of modified membranes demonstrated high performance in MTBE removal from its aqueous mixture. Among the membranes studied, CNIM-f provided the best performance in terms of flux, removal efficiency, mass transfer coefficients and overall selectivity. The immobilization f-CNTs and GO altered the surface characteristics of the membrane and enhanced partition coefficients, and thus assisted MTBE transport across the membrane. The MTBE flux reached as high as 1.4 kg/m2 h with f-CNTs, which was 22% higher than that of the unmodified PTFE membrane. The maximum MTBE removal using CNIM-f reached 56% at 0.5 wt % of the MTBE in water, and at a temperature of 30 °C. With selectivity as high as 60, MTBE recovery from contaminated water is very viable using these nanocarbon-immobilized membranes.
Emerging 2D Materials and Their Van Der Waals Heterostructures
Authors: Di Bartolomeo Antonio
Published: March 2020

Two-dimensional (2D) materials and their van der Waals heterojunctions offer the opportunity to combine layers with different properties as the building blocks to engineer new functional materials for high-performance devices, sensors, and water-splitting photocatalysts. A tremendous amount of work has been done thus far to isolate or synthesize new 2D materials as well as to form new heterostructures and investigate their chemical and physical properties. This article collection covers state-of-the-art experimental, numerical, and theoretical research on 2D materials and on their van der Waals heterojunctions for applications in electronics, optoelectronics, and energy generation.