bluuebunny/crossref_metadata_2025 huggingface dataset:
Celiac disease: Definition, classification, historical and epistemological profiles, anatomopathological aspects, clinical signs, differential diagnosis, treatments and prognosis. Proposed diagnostic scheme for celiac disease (DSCNC)
Authors: Giulio Perrotta , Emanuele Guerrieri
Published: May 2022

Celiac disease is an immune-mediated enteropathy, caused (in genetically predisposed or susceptible individuals) by the ingestion of gluten, the complex of water-insoluble proteins found in cereal grains such as wheat, rye and barley. In terms of terminology, it is the complex natural history and extremely polymorphous clinical presentation that has created some confusion. In fact, to date, at least three different forms of celiac disease are known, in addition to the simple non-celiac gluten sensitivity, since in common clinical practice most patients do not present the classic symptoms such as malabsorptive syndrome with diarrhoea, steatorrhoea, weight loss and nutritional deficiency, but rather an anaemia, asthenia, meteorism, abdominal tension, osteoporosis and infertility, thus painting an extremely varied and complex symptomatic picture that is linked to enteric microbiota and microbiome issues. Celiac disease affects the mucosa of the small intestine, while it generally spares the submucosa, muscolaris propria and serosa; if the disease does not involve the whole of the small intestine but only part of it, it is usually more serious in the proximal than the distal tract. The simultaneous presence of shortened villi, crypt hyperplasia, the abnormal cytological appearance of the absorbent surface and increased lamina propria cells is required for the diagnosis of celiac disease. Based on these findings, several forms of celiac disease have been identified in the clinic: typical, atypical (and in turn silent, latent, and potential), and sensitive non-celiac. Based on these considerations a specific diagnostic scheme is suggested to frame the celiac universe more functionally and structurally (so-called Diagnostic Scheme for Celiac Disease and Nonceliac Gluten Sensitivity, DSCNC), identifying at least eight clinical hypotheses based on the serological, genetic, bioptic and allergological tests suggested). From a pharmacological and integrative point of view, the protocols shared by the scientific community remain in place: gluten-free diet, vitamin and salt supplementation if appropriate, pharmacological therapy (antibiotics, antihistamines, corticosteroids and immunosuppressants) if necessary, also in the future with the majority orientation oriented towards oral glutenase able to counteract the effects of gliadin in sensitive subjects, the use of larazotide acetate to remedy the increase in intestinal permeability and tTG inhibitors to reduce the toxic effects of gluten intake. The state of the art on celiac disease is not yet able to explain the precise aetiology and atypical forms of the disease, as well as the real impact of genetic predisposition on clinical manifestations. Research continues and seems to point the way to a complete resolution of this enteropathy that has been so prevalent over the last two decades.
Pure Relief CBD Gummies Reviews - Price, benefits, Side Effects v1
Authors: not provided purereliefcbd
Published: May 2022

Pure Relief CBD Gummies Reviews - Price, benefits, Side Effects
isolation and extraction of plant nuclei in plug v1
Authors: Labadie Karine , Vacherie Benoît
Published: May 2022

Method for isolation and extraction of plant cell nuclei. Protocol for obtaining UHMW DNA (> 150kb) allowing the production of optical cards with Bionano technology.
Insect Cell Protocol for LRRK1 and LRRK2 Expression v1
Authors: Xuan Lin Yu
Published: May 2022

Protocol for expressing LRRK1 and LRRK2 in insect cells. As used in Snead, Matyszewski, Dickey et al. 2022.
Quantification of the SARS-CoV-2 using electronegative membrane filtration and dPCR v1
Authors: Narum Solana , Stalder Thibault
Published: May 2022

This protocol was developed in an effort to serve as a timely and efficient method for the surveillance of the SARS-CoV-2 in primary influent wastewater samples. This process describes viral concentration via electronegative adsorption with pH adjustment, RNA extraction using the MP Biomedicals FastPrep-24 and the Qiagen AllPrep PowerViral DNA/RNA kits with the Qiacube Connect, and quantification of the N1 and N2 genes in SARS-CoV-2 using the GT Digital SARS-CoV-2 Wastewater Surveillance for QIAcuity. To compile the entire process from beginning to the end some sections were taken from the AllPrep PowerViral DNA/RNA Kit (Qiagen) and the GT Digital SARSCoV- 2 Wastewater Surveillance for QIAcuity v1.0 (GT Molecular) handbooks.
P4v3 v3
Authors: not provided maria
Published: May 2022

Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Pellentesque arcu. Phasellus faucibus molestie nisl. Quis autem vel eum iure reprehenderit qui in ea voluptate velit esse quam nihil molestiae consequatur, vel illum qui dolorem eum fugiat quo voluptas nulla pariatur? Suspendisse sagittis ultrices augue. Vestibulum erat nulla, ullamcorper nec, rutrum non, nonummy ac, erat. Nullam justo enim, consectetuer nec, ullamcorper ac, vestibulum in, elit. In convallis. Mauris elementum mauris vitae tortor. Sed ac dolor sit amet purus malesuada congue. Etiam ligula pede, sagittis quis, interdum ultricies, scelerisque eu. Nunc auctor. Nam sed tellus id magna elementum tincidunt. Sed convallis magna eu sem. Quis autem vel eum iure reprehenderit qui in ea voluptate velit esse quam nihil molestiae consequatur, vel illum qui dolorem eum fugiat quo voluptas nulla pariatur? In dapibus augue non sapien.
CODEX Staining Protocol For FFPE Tissue v1
Authors: J Ahn Kyung , Bandyopadhyay Shovik , Thadi Anusha , Tan Kai
Published: May 2022

This protocol describes the method for antibody staining of FFPE tissues on coverslips using CODEX Barcoded Antibodies. The Akoya CODEX user manual was modified to include photobleaching steps. Included are the stepwise protocols for pre-staining, deparaffinization, antigen retrieval, photobleaching, antibody staining and post-fixation.
DNA extraction from insect gut-dwelling fungi v1
Authors: Wang Yan
Published: May 2022

This protocol is good for DNA extraction from microbial fungi isolated from aquatic insect guts. It works for small input tissues (starting from one fungal thallus). The product can be used for PCR and Sanger sequencing directly, which is ideal for fungal barcode analyses. This protocol is modified from a method described in Gottlieb and Lichtwardt 2001 (Mycologia Vol. 93, No. 1, pp. 66-81).
Human myocardium decellularization v1
Authors: Belviso Immacolata , Maria Sacco Anna , Cozzolino Domenico , Nurzynska Daria , Di Meglio Franca , not provided clotilde.castaldo , Romano Veronica
Published: May 2022

The protocol represents a step-by-step method to obtain a decellularized cardiac matrix through the combination of sodium dodecyl sulphate (SDS) and Triton X-100. Briefly, cardiac samples obtained from left ventricles of explanted, pathological human hearts were dissected and washed to remove residual body fluids. Samples were then snap-frozen and sliced by a cryostat into 350 µm thick sections. The sections obtained were decellularized using a solution containing 1% Triton X-100 and 1% SDS in combination, for 24 hours, until observing the color change from brownish-red to translucent-white. As a result, the protocol shows efficiency in preserving ECM architecture and protein composition during the whole process, suggesting that it is worthwhile, highly reproducible and produces a well- preserved decellularized extracellular matrix from cardiac samples.
Photo-oxidation Using MiniSOG with EM Preparation of Transfected Culture Cells v1
Authors: Mackey Mason , Shu Xiaokun , Lev-Ram Varda , J. Deerinck Thomas , Qi Yingchuan , B. Ramko Ericka , W. Davidson Michael , Jin Yishi , H. Ellisman Mark , Y. Tsien Roger
Published: May 2022

Abstract taken from Plos Biology Journal: A Genetically Encoded Tag for Correlated Light and Electron Microscopy of Intact Cells, Tissues, and Organisms Electron microscopy (EM) achieves the highest spatial resolution in protein localization, but specific protein EM labeling has lacked generally applicable genetically encoded tags for in situ visualization in cells and tissues. Here we introduce “miniSOG” (for mini Singlet Oxygen Generator), a fluorescent flavoprotein engineered fromArabidopsisphototropin 2. MiniSOG contains 106 amino acids, less than half the size of Green Fluorescent Protein. Illumination of miniSOG generates sufficient singlet oxygen to locally catalyze the polymerization of diaminobenzidine into an osmiophilic reaction product resolvable by EM. MiniSOG fusions to many well-characterized proteins localize correctly in mammalian cells, intact nematodes, and rodents, enabling correlated fluorescence and EM from large volumes of tissue after strong aldehyde fixation, without the need for exogenous ligands, probes, or destructive permeabilizing detergents. MiniSOG permits high quality ultrastructural preservation and 3-dimensional protein localization via electron tomography or serial section block face scanning electron microscopy. EM shows that miniSOG-tagged SynCAM1 is presynaptic in cultured cortical neurons, whereas miniSOG-tagged SynCAM2 is postsynaptic in culture and in intact mice. Thus SynCAM1 and SynCAM2 could be heterophilic partners. MiniSOG may do for EM what Green Fluorescent Protein did for fluorescence microscopy. https://doi.org/10.1371/journal.pbio.1001041
prime-seq v2
Authors: Janjic Aleksandar , Esteban Wange Lucas , JWB Bagnoli Johannes , Geuder Johanna , Nguyen Phong , Richter Daniel , Ziegenhain Christoph , Enard Wolfgang , Vieth Beate
Published: May 2022

Cost-efficient library generation by early barcoding has been central in propelling single-cell RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method. We show that it performs equivalently to TruSeq, a standard bulk RNA-seq method, but is fourfold more cost-efficient due to almost 50-fold cheaper library costs. We also validate a direct RNA isolation step, show that intronic reads are derived from RNA, and compare cost-efficiencies of available protocols. We conclude that prime-seq is currently one of the best options to set up an early barcoding bulk RNA-seq protocol from which many labs would profit.
P sharing 1 v1
Authors: not provided maria
Published: May 2022

test
P1 sharing w/ ws v1
Authors: not provided maria
Published: May 2022

test
Photo-oxidation Using MiniSOG with EM Preparation of Transfected Culture Cells v2
Authors: Mackey Mason , Shu Xiaokun , Lev-Ram Varda , J. Deerinck Thomas , Qi Yingchuan , B. Ramko Ericka , W. Davidson Michael , Jin Yishi , H. Ellisman Mark , Y. Tsien Roger
Published: May 2022

Abstract taken from Plos Biology Journal: A Genetically Encoded Tag for Correlated Light and Electron Microscopy of Intact Cells, Tissues, and Organisms Electron microscopy (EM) achieves the highest spatial resolution in protein localization, but specific protein EM labeling has lacked generally applicable genetically encoded tags for in situ visualization in cells and tissues. Here we introduce “miniSOG” (for mini Singlet Oxygen Generator), a fluorescent flavoprotein engineered fromArabidopsisphototropin 2. MiniSOG contains 106 amino acids, less than half the size of Green Fluorescent Protein. Illumination of miniSOG generates sufficient singlet oxygen to locally catalyze the polymerization of diaminobenzidine into an osmiophilic reaction product resolvable by EM. MiniSOG fusions to many well-characterized proteins localize correctly in mammalian cells, intact nematodes, and rodents, enabling correlated fluorescence and EM from large volumes of tissue after strong aldehyde fixation, without the need for exogenous ligands, probes, or destructive permeabilizing detergents. MiniSOG permits high quality ultrastructural preservation and 3-dimensional protein localization via electron tomography or serial section block face scanning electron microscopy. EM shows that miniSOG-tagged SynCAM1 is presynaptic in cultured cortical neurons, whereas miniSOG-tagged SynCAM2 is postsynaptic in culture and in intact mice. Thus SynCAM1 and SynCAM2 could be heterophilic partners. MiniSOG may do for EM what Green Fluorescent Protein did for fluorescence microscopy. https://doi.org/10.1371/journal.pbio.1001041
Virtual Screening v1
Authors: Sciences BOC
Published: May 2022

Virtual screening has been widely applied in early-stage drug discovery. As an alternative or complementary approach to high-throughput screening (HTS) assays with high cost and low hit rate, virtual screeningis an efficient computational method to identify drug candidates in silico from large chemical compound databases. Its usefulness has been verified by current applications that successfully retrieved hit and lead identifications against various disease targets.
ATLANTIS: An adaptive multi-arm phase II trial of maintenance targeted therapy after chemotherapy in advanced/metastatic urothelial cancer - Cabozantinib arm v1
Authors: Powles Thomas , J Jones Robert , Crabb Simon
Published: May 2022

Background: Metastatic urothelial cancer (MUC) is the eighth most common cause of cancer death in the UK. Chemotherapy is standard treatment for MUC. Although MUC is initially sensitive to chemotherapy, patients usually relapse within 6 months of completion. The outcome, once relapse has occurred, is very poor (median overall survival 8 months). Therefore, maintaining response to chemotherapy is an attractive goal, and a good environment for testing active single agents in MUC. ATLANTIS is a multi-arm, multi-stage signal searching trial. Patients will be allocated to treatment (vs observation or placebo where possible) based on biomarker expression. Design: ATLANTIS is a randomised phase II biomarker directed umbrella screening trial of maintenance therapy in biomarker defined subgroups of patients with advanced or metastatic urothelial cancer. The primary endpoint is progression-free survival. The protocol consists of a generic main section plus novel drug-specific appendices. The relevant novel drug-specific appendices will be amended when each specific subgroup trial completes or when a further novel drug subgroup is introduced. Central tissue screening for biomarker will occur prior to randomisation. Objectives: The trial will investigate whether maintenance targeted therapy after chemotherapy, with treatment selection based on biomarker expression, delays time to progression and overall survival for patients with MUC. Endpoints: Primary: To compare progression-free survival (PFS) of trial drug and placebo/observation. Different drugs will be tested in different biomarker defined subgroups in an adaptive design. PFS has been chosen as the primary end-point as it is largely objective (the majority of urothelial cancer patients will display progression in accordance with RECIST1.1 criteria) and clinically meaningful - progression after first line therapy represents the transition to the lethal stage of the disease and often the requirement for further cytotoxic therapy. Secondary: Overall survival, response rate, maximum percentage decrease in measurable disease, safety and tolerability. Exploratory: Progression free survival in subgroups defined by biomarkers other than those used for treatment allocation. Translational: Archival tissue, plasma and circulating tumor DNA will be stored and used for future research.
Interferon signalling as a potentional therapeutic target in amyotrophic lateral sclerosis and frontotemporal dementia – protocol for a systematic review and meta-analysis v1
Authors: M Waldron Fergal , Rifai Olivia , Gregory Jenna
Published: May 2022

Amyotrophic lateral sclerosis (ALS) is a rapidly progressive neurodegenerative disease with no cure and limited treatment options. There is therefore an urgent need for effective therapeutic interventions in this disease. This protocol outlines the strategy for a systematic review and meta-analysis to identify, from in vivo animal studies, potential therapeutic interventions for ALS cases in which an interferon response is induced. Our aim is to perform a comprehensive review of the ALS literature to compile a list of (i) interventions affecting interferon signalling pathways and (ii) specific interferon signalling pathway targets that may be manipulated for therapeutic benefit in patients with ALS. We will do this by assessing the pre-clinical literature for the effects of interferon signalling pathway manipulation on the following primary and secondary outcome measures comparing models of ALS/FTD to controls; the primary outcome measure being survival, and secondary outcome measures including histological, biochemical, and behavioural metrics. We will also carry out a structured quality assessment of the literature to provide recommendations for future studies.
High time-resolution of root tracking of Arabidopsis roots using SPIRO, SmartRoot and BackTracker v1
Authors: M Julkowska Magdalena
Published: May 2022

This protocol describes an alternative way of analyzing SPIRO images for root growth of plants that are transferred to the treatment plates - and thus not imaged from the germination onwards. This protocol combines SPIRO tools, SmartRoot and BackTracker script, as well as R-scripts for data curation. The protocol was thus far tested on Arabidopsis roots - and we intend to test it in the future on tomato roots as well.
Liquid biopsy in sentinel mussels v1
Authors: Ferchiou Sophia , Caza France , St-Pierre Yves
Published: May 2022

This protocol has been optimized for sampling hemolymph in sentinel mussels in remote areas, such as polar regions. A detailed protocol can be found in Caza et al. (2019).
Plant assemble - Plant de novo genome assembly: scaffolding v2
Authors: Ferguson Scott , Jones Ashley , Borevitz Justin
Published: May 2022

With the advancement of long-read sequencing technologies and associated bioinformatics tools, it has now become possible to de novo assemble complex plant genomes with unrivalled contiguity, completeness and correctness. As read lengths can surpass repeat lengths, the ability to assemble genomes de novo has dramatically improved, whereby complex plant genomes of widely variable sizes and repeat content have highly benefited. Despite these improvements, challenges remain in performing de novo assembly, namely in developing a reliable workflow and in tool choice. Here we present a protocol collection of bioinformatic workflows detailing plant genome assembly using Oxford Nanopore Technologies long-reads with a de novo assembler (Canu), syntenic or Hi-C scaffolding, and RNA and/or gene homology-based annotation. We have developed and tested these protocols on multiple plant genomes. Using these protocols with sufficient coverage of long-reads, a highly contiguous, complete, and correct plant genome can be assembled. These genomes can further genomic research into structural variation among groups, and SNP genotyping and association studies among populations.
A fluorescence-based in vitro scrambling assay for yeast MCP1 and human XK v1
Authors: Hong Zhouping , Reinisch Karin
Published: May 2022

VPS13 proteins are proposed to function at contact sites between organelles as bridges for lipids to move directionally and in bulk between organellar membranes. VPS13s are found to interact with integral membrane proteins, like MCP1 in yeast or XK in humans. We showed that MCP1 and XK scramble phospholipids in vitro. Here I describe the detailed procedure of purification, reconstitution, and scrambling assay for both MCP1 and XK.
FLASH-seq UMI protocol v3
Authors: Picelli Simone , Hahaut Vincent
Published: May 2022

The single-cell RNA-sequencing (scRNA-seq) field has evolved tremendously since the first paper was published back in 2009. While the first methods analysed just a handful of cells, the throughput and performance rapidly increased over a very short timespan. However, it was not until the introduction of emulsion droplets methods, that the robust and reproducible analysis of thousands of cells became feasible. Despite generating data at a speed and a cost per cell that remains unmatched by full-length protocols like Smart-seq, scRNA-seq in droplets still comes with the drawback of addressing only the terminal portion of the transcripts, thus lacking the required sensitivity for comprehensively analyzing the transcriptome of individual cells. Building upon the existing Smart-seq2/3 workflows, we developed FLASH-seq (FS), a new full-length scRNA-seq method capable of detecting a significantly higher number of genes than both previous versions, requiring limited hands-on time and with a great potential for customization.
Overview of scRNA-seq of Human Knee Articular Cartilage v1
Authors: not provided molmer , Lotz Martin
Published: May 2022

The overview provides sub-protocols for each step required to complete scRNA-seq from human knee articular cartilage.
Characterization of osmoregulated periplasmic glucans with high resolution LCMS v1
Authors: not provided lcmsmethods , C. Orsburn Benjamin , Eberhard Colten , Daitsch Allison , N. Bumpus Namandje
Published: May 2022

Characterization of osmoregulated periplasmic glucans with high resolution LCMS
F0 knockout—single gene v3
Authors: Kroll Francois , Rihel J
Published: May 2022

Please cite 10.7554/eLife.59683 if you use this protocol. Note; our eLife publication used Version 2 of this protocol. Later version are subsequent improvements/simplifications. Get in touch for questions/suggestions twitter –@francois_kroll email –francois@kroll.be
Knee Cartilage Grading Protocol v1
Authors: not provided molmer , Lotz Martin
Published: May 2022

The attached document shows the Gross Morphological Assessment Sheet we use to score and grade knee cartilage.
Identifying retrograde labelled colon and bladder afferent neurons in the sensory ganglia v1
Authors: not provided Andrea.harrington
Published: May 2022

Identifying retrograde labelled colon and bladder afferent neurons in the sensory ganglia
FQ-LAMP Assay for Detection of CoV2 in Clinical Nasal Swabs v1
Authors: Jones Les , K. Naikare Hemant , C. Mosley Yung-Yi , Ralph A. Tripp and
Published: May 2022

COVID-19 is a public health challenge requiring rapid testing for detecting infections and transmission. Nucleic acid amplification tests (NAAT) targeting SARS-CoV-2 (CoV2) are used to detect CoV2 in clinical samples.Real-time reverse transcription-quantitative PCR (RT-qPCR) is the standard NAAT for CoV2, although Reverse Transcription Loop-mediated isothermal amplification (RT-LAMP) is used in diagnostics. We show a sequence-specific RT-LAMP-based NAAT assay that is finished within 30 min using minimally processed clinical nasal swab samples and describe a fluorescent-quenched RT-LAMP assay (FQ-LAMP) using labeled primers and a quencher oligo. This assay can achieve rapid (30 min) and sensitive (1000 PFU/ml) fluorescent detection of CoV2 (WA1/2020), B.1.1.7 (Alpha), and variants of concern Delta (B.1.617.2) and Omicron (B.1.1.529) in nasal samples.
Donor Eligibility Criteria and Pancreas Recovery for HuBMAP TMC-PNNL-UF v1
Authors: Chen Jing , Campbell-Thompson Martha , E Mathews Clayton
Published: May 2022

This document outlines inclusion and exclusion criteria for pancreas donors for the Human BioMolecular Atlas Program (HuBMAP) by the Tissue Mapping Center (TMC) at the University of Florida and pancreas recovery procedures to be conducted by the Organ Procurement Organization Recovery Team. A member of the HuBMAP TMC-PNNL-UF team will accept pancreas offers using standardized inclusion and exclusion criteria for equal numbers of female and male donor with consideration for transportation issues that could delay receipt at the University of Florida. Redacted documents pertinent to donor and hospitalization course will be obtained and data entered into HuBMAP HIVE portal.
DRG stimulation - synchronized nerve and DRG v1
Authors: Feng Bin , not provided longtu.chen
Published: May 2022

Assessing the instantaneous effect of DRG stimulation on the transmission of electrically evoked action potentials from the spinal nerve via the ex vivo preparation with mouse L6 spinal nerve, DRG, and dorsal root in continuity, ie, the SN-DRG-DR preparation.
Reconstitution of LRRK2 membrane recruitment onto planar lipid bilayers v1
Authors: Adhikari Ayan
Published: May 2022

Supported lipid bilayers have emerged as an ideal model system to study the interaction of proteins with cellular membranes. We describe here a method to monitor the recruitment of purified LRRK2 kinase onto planar lipid bilayers containing lipid-anchored Rab10 protein using Total Internal Reflection Fluorescence (TIRF) Microscopy.This method utilizes purified, FLAG-tagged, full length LRRK2 labeled with CF633 succinimidyl ester (Biotium) and bacterially expressed eGFP-Rab10-His tagged protein. LRRK2 recruitment is captured in real time at 25°C using a Nikon Ti-E inverted microscope with an Andor iXon+EMCCD camera model DU885, with PerfectFocus and a Nikon TIRF Apo 100X 1.46 NA oil immersion objective.
Mouse Whole Cell Tissue Processing for 10x Genomics Platform v9
Authors: Institute for Brain Science Allen
Published: May 2022

This protocol describes how to isolate tissue from various ROI’s of the mouse brain followed by preparation of a highly concentrated single cell suspension for transcriptomic profiling on 10x Genomics platform. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
scRNA-seq Data Processing (Cell Ranger, v6.0.0) v1
Authors: not provided molmer , Lotz Martin , Natarajan Padmaja , Head Steven
Published: May 2022

scRNA-seq data are analyzed using Cell Ranger from 10X Genomics.
Human fibroblast culturing v1
Authors: Smith Laura , C David
Published: May 2022

Fibroblasts are cultured in Dulbecco’s modified eagle media (DMEM) 4500 (mg/L) growth medium supplemented with Glutamax (Gibco), 10% foetal bovine serum (FBS), non-essential amino acids (NEAA: 0.1 mM of: glycine, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, L-proline and L-serine) and penicillin/streptomycin antibiotic cocktail (50ng/ml) at 37°C and 5% CO2.
wrist pulse wave signal v1
Authors: not provided Anonymous , Yao RuiLing
Published: May 2022

In previous studies, pulse signals have been analysed primarily to standardise and objectify pulse diagnosis, but the influence of TCM constitution on pulse signals and disease has been neglected. Our study links the wrist pulse wave signal to TCM constitution to find TCM constitution factors that predispose to disease while improving the accuracy of disease classification. Our specific approach is set out below. A wrist pulse signal sampler based on a piezoelectric sensor was designed and 95 healthy subjects and 20 hypertensive patients were invited to complete the CCMQ (Constitution in the Chinese Medicine Questionnaire), of which 48 completed the pulse wave signal. Meanwhile, we improved the Butterworth filtering algorithm to denoise the pulse wave signals, and after smoothing and period segmentation, we finally extracted 27 time-domain features and 8 wavelet packet energy features. Then, we used the independent sample t-test and binary logistic regression analysis to analyze the correlation between the prevalence of hypertension and TCM constitution and found that the three constitutions of YaD (Yang-deficiency), YiD (Yin-deficiency), and PW (Phlegm-wet) were significantly associated with whether the subjects suffered from hypertension. The final classification results showed that the fusion features after adding the three constitution features with a high correlation with hypertension had a 17.46% higher F-score than the classification model without the constitution features. It can be seen that the incorporation of constitution characteristics into pulse characteristics in this paper has important implications for disease classification. This also provides a basis for studying the correlation between TCM physical characteristics and certain diseases, and patients can adopt targeted TCM management techniques to prevent diseases in advance.
Preparation of Triton X-100 soluble and insoluble fractions of SH-SY5Y cells v1
Authors: Smith Laura
Published: May 2022

SH-SY5Y cells are trypsinised and lysed in 1% (v/v) Triton X 100, 50 mM Tris, pH 7.5, 750 mM NaCl, 5 mM EDTA, 4 units RQ1 RNase-free DNase (Promega), protease and phosphatase inhibitor mix (Halt) on ice for 15 minutes. The lysate is passed through a 23G needle and pelleted at 17,000 x g for 30 minutes at 4°C. Triton X 100 soluble fraction is collected and protein concentration measured using the BCA protein assay. Insoluble pellets are resuspended in 2% (w/v) SDS, 8 M urea, 10 mM Tris, pH 7.5, 4 units RQ1 RNase-free DNase, protease and phosphatase inhibitor mix and incubated for 15 minutes at room temperature. Debris is removed by centrifugation at 17,000 x g for 30 minutes at 4°C.
Isolation, cryo-laser scanning confocal microscope imaging and cryo-FIB milling of mouse glutamatergic synaptosomes v1
Authors: Gogoi Prerana
Published: May 2022

Ionotropic glutamate receptors (iGluRs) at postsynaptic terminals mediate the majority of fast excitatory neurotransmission in response to release of glutamate from the presynaptic terminal. Obtaining structural information on the molecular organization of iGluRs in their native environment, along with other signaling and scaffolding proteins in the postsynaptic density (PSD), and associated proteins on the presynaptic terminal, would enhance understanding of the molecular basis for excitatory synaptic transmission in normal and in disease states. Cryo-electron tomography (ET) studies of synaptosomes is one attractive vehicle by which to study iGluR-containing excitatory synapses. Here we describe a workflow for the preparation of glutamatergic synaptosomes for cryo-ET studies. We describe the utilization of fluorescent markers for the facile detection of the pre and postsynaptic terminals of glutamatergic synaptosomes using cryo-laser scanning confocal microscope (cryo-LSM). We further provide the details for preparation of lamellae, between ~100 to 200 nm thick, of glutamatergic synaptosomes using cryo-focused ion-beam (FIB) milling. We monitor the lamella preparation using a scanning electron microscope (SEM) and following lamella production, we identify regions for subsequent cryo-ET studies by confocal fluorescent imaging, exploiting the pre and postsynaptic fluorophores.
Team Deleidi | Protocols | "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly v1
Authors: not provided michela.deleidi , José Pérez J. María , Baden Pascale , Raji Hariam , Bertoli Federico
Published: May 2022

Collection of protocols of Team Deleidi used in the publication: "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function"
[RETRACTED] Titan XL Male Enhancement of 2022 Restore Your Masculinity And Manhood v1
Authors: not provided officialtitanxl
Published: May 2022

[RETRACTED]

Titan XL Male Enhancement is a nutritional supplement sold online through titanxl.com. The supplement is marketed to men who want to increase the length of their penis through natural methods permanently.According to titanxl.
[RETRACTED] Titan XL Male Enhancement (WORKS & HOAX) For That Body Requires During Bed Performance. v1
Authors: not provided haiannise
Published: May 2022

[RETRACTED]

Titan XL
[RETRACTED] Keto Start ACV - (Pros and cons) Results, Price, ACV Start v1
Authors: not provided officialmiloparker
Published: May 2022

[RETRACTED]

Keto Start ACVare the option to eliminate all the bipolar and health affecting factors from the body. Most of the individuals are dealing with stress and insomnia issues.
PNA-lipophilic Ligand Conjugates Synthesis v1
Authors: Miller Cathy
Published: May 2022

Lipophilic ligands are a class of substances suitable for cell transport, they are lipids with a certain hydrophilic-lipophilic balance. When transported across the cell membrane, the hydrophilic end faces the extracellular water-soluble environment, and the hydrophobic end faces the cell membrane. A typical representative of this modification is the covalent attachment of bile acid and cholesterol to PNA. The conjugate also has a certain liver-targeting effect in vivo and has the potential to develop into a liver-targeted preparation. The covalent binding of PNA with hydrophobic compounds or lipids has some excellent properties. For example, by connecting a hydrophobic long carbon chain to the N-terminus of PNA and amino acid residues to the C-terminus, the conjugate has good hydrophilicity and lipophilicity, and shows better specificity when hybridizing with DNA. The conjugate can also self-assemble into spherical micelles, and has application potential in biosensors, gene drugs, etc. PNA-lipophilic ligand conjugate also shows good performance in cell transport. Creative Peptideshas completed many PNA conjugate synthesis cases. Based on these projects, our team has accumulated a deep understanding of product requirements. With rich experience and scientific knowledge, our experts are dedicated to helping our customers navigate and accelerate your PNA synthesis plan. By combining scientific knowledge and laboratory experience, we are committed to expanding your understanding and applications of PNA conjugates. Pleasecontact usto learn more.
SCOT – Short Course Oncology Therapy – A Study of Adjuvant Chemotherapy in Colorectal Cancer v1
Authors: Iveson Timothy , Saunders Mark , Harkin Andrea , Kelly Caroline , Boyd Kathleen
Published: May 2022

Design: Phase III, randomised controlled, two arm, open label, multicentre, non-inferiority. Objectives: Assessment of the efficacy of 12 weeks versus 24 weeks of treatment and associated toxicity. Economic analysis of the cost effectiveness of the 2 arms. Comparison of 2 randomisation methodologies. Endpoints: Primary Endpoint: • Disease free survival Secondary Endpoints: • Overall survival • Cost-effectiveness • Toxicity • Quality of Life Study population: 6087 patients with fully resected high-risk stage II or fully resected stage III colorectal cancer.
First Strand cDNA Synthesis Kit using ProtoScript II Reverse Transcriptase (M0368) v2
Authors: England Biolabs New
Published: May 2022

This protocol explains methods for the "First Strand cDNA Synthesis Kit using ProtoScript II Reverse Transcriptase (M0368)".
Large-Scale Stool Homogenization v1
Authors: C Dillon Katie , R Hensley Jasmine , A Aspinwall Brooke , T Lucking Sean , Carleton Heather , D Huang Andrew , J Williams-Newkirk Amanda
Published: May 2022

Purpose This document outlines the procedure for preparing a homogenized human stool specimen. A homogenized sample ensures specimen uniformity for controlled experiments and research. Scope This document applies to the homogenization of large fecal specimens (> 20 g). Related Documents (see attachment)
DNA Extraction from Modern Dental Plaque on Gauze v1
Authors: Aron Franziska , Velsko Irina
Published: May 2022

Protocol for DNA extraction from modern dental plaque dried on gauze samples for Illumina sequencing. This protocol uses the Qiagen PowerSoil DNA extraction kit, but applies modifications to improve DNA recovery. Specifically, solution C2 is not used to avoid uneccessary DNA loss during inhibitor removal.
Pyruvate and Urea preparation for dissolution DNP v1
Authors: Korenchan Dave , Yoshihara Hikari , Subramaniam Sukumar , DeLos Santos Romelyn , Sriram Renuka , Kurhanewicz John
Published: May 2022

This document codifies the probe preparation of pyruvate and urea for dissolution dynamic nuclear polarization. Additionally, this also describes the steps recommended for estimation of T1 and polarization and quality assurance to ensure repeatability and robustness of hyperpolarized 13C probe formulation.
CODEX Imaging Setup and ProcessingExperiment Protocol v1
Authors: not provided Kavya.Anjani , not provided Miguel.Rivera , not provided Zoltanlaszik
Published: May 2022

CODEX system is the combination of an (1) oligo-nucleotide based antibody labeling-detection technique, (2) a microfluidics instrument coupled with an inverted microscope capable of whole slide scanning, and an (3) ImageJ-based analysis platform. This experimental setup allows the automation of immunofluorescence signal detection across several staining cycles of a single tissue section This protocol describes detailed methods used in CODEX imaging experiments at the Laszik Lab.
Molecular cloning of SHIP164 plasmids for expression in mammalian cells v1
Authors: not provided michael.hanna , G G Hanna Michael , De Camilli Pietri
Published: May 2022

This protocol is to help with the molecular cloning of SHIP164 and the sequences of other proteins.
Cell culture, transfection, immunocytochemistry, and imaging v1
Authors: G G Hanna Michael , De Camilli Pietro
Published: May 2022

This protocol is to help with the maintenance, transfection, immunocytochemistry, and imaging of adherent mammalian cells associated with the publication DOI above.
4D Upper Gastrointestinal Magnetic Resonance Imaging in Healthy Human Subjects and Gastroparetic Patients v1
Authors: Lu Kun-Han , Mosier Kristine , Wo John , Powley Terry
Published: May 2022

This protocol described the steps to acquire and analyze 4D gastric magnetic resonance imaging data under fast and fed states in healthy human subjects and patients with gastroparesis.
PROTAC Design based on Bioinformatics v1
Authors: not provided boc.protac
Published: May 2022

In order to simplify and accelerate the discovery of PROTAC, it is necessary to grasp the design rules of PROTAC, which is helpful to establish a reliable PROTAC evaluation platform. To date, most reported PROTAC designs are based on target protein-binding ligands (usually inhibitors). Because the crystal complex structure of the target protein and its binding ligand and the structure-activity relationship information of the ligand have been used to guide PROTAC design, such as determining the Linker binding site of the target protein binding ligand. Recent research uses bioinformatics to guide PROTAC optimization. Bioinformatics methods play an irreplaceable role in the process of drug discovery, especially for the analysis of large-scale multi-group data. at present, many disease-related database resources have emerged, and a variety of bioinformatics methods have been established to discover potential drug targets based on biological network characteristics, multi-gene chip, proteome, metabolome data and so on. The target drug availability and drug side effects were predicted. As a leading service provider in drug discovery and research, BOC Sciences is fully capable and committed to providing one-stop proteolysis targeting molecular drug discovery based on chimeric (PROTAC®). With a comp rehensive and advanced platform, we provides PROTACdesign based on bioinformaticsservices to customers around the world to meet new drug discovery goals.
[RETRACTED] Keto Start ACV 2022 Tested? : Is It the Right Product For You? v1
Authors: not provided furiosgnat
Published: May 2022

[RETRACTED]

Keto Start ACV Reviews: Read Ingredients, Benefits, Use, Warnings, Shark Tank Scam, and where to buy Keto Start ACV? Reduce extra body weight with a nutritive option! Read the article to explore if it is legit or a scam!
Removal and Omission of Fiction Synesthetic Metaphors in English-Ukrainian Translation
Authors: Zhulavska Olha
Published: May 2022

This paper investigates omission and removal of synesthetic metaphoric descriptions in English-Ukrainian translations of Celeste Ng’s novel “Everything I Never Told You”. A synesthetic metaphor is viewed as a product of mapping between two different domains of human experience, which are both of sensory nature. The mappings instantiate in synesthetic metaphoric descriptions. The study resorts to the methodological tools of cognitive metaphor translation theory and the affordances of corpus linguistics and quantitative analysis to reveal the correlation between the choice of removal/omission of the source-text synesthetic metaphoric descriptions and the linguacultural specificity/universality of their metaphorical mappings. The results suggest that omission/removal is explained by the linguacultural features of the source-target text cross-sensory mappings and the translators’ free choices. Omission can be accounted for by a translator’s choice to avoid redundancy. Removal occurs if the target-text equivalent synesthetic metaphoric description a) is based on a cross-sensory mapping not embedded in the target linguaculture, b) rests on a different semantic specification of a structurally identical cross-sensory mapping, or c) has a lower degree of conventionality than its non-metaphoric periphrasis.
Tracing Western Transnationalism in Indian Graphic Novels
Authors: Sivakumar Abinaya , Patchainayagi S.
Published: May 2022

The essence of transnationalism is quite dominant in the post-millennial period. It is concerned with the idea of a hybrid society in which diverse cultures swiftly trade their rich cultural history, resulting from the various exchanges brought on by globalisation. Following its expanding economic and political changes, India is now moving forward with its own unique creation of entertainment endeavours. In the present post-millennial era, India is beginning to produce its own graphic novels. This research examines the steps that have helped Indian graphic novels attain extreme commercial success. The paper also discusses how authors like Sarnath Banerjee, Amruta Patil, Malik Sajad, and Vishwajyothi Ghosh truthfully highlight various aspects of Indian society. This study tries to comprehend what it means to be authentically Indian by tracing the presence of transnationalism in Indian graphic novels by Sarnath Banerjee, Amruta Patil, Vishwajyothi Gosh, and Malik Sajad.
The Role of Conversational Implicature in Daily Conversations – What Matters, Content or Context?
Authors: Musa Rabab Elsheikh Idris , Mohammed Bahia K.
Published: May 2022

In daily conversation, sometimes dialogue includes terms that vary entirely from the common phrases. From a linguistic perspective, the conversational implicatures are the speaker's intended meaning of the utterance. Conversation implications are the specific conversations between the speaker and the receiver by following the communications principles. It is the most significant component that has undergone argumentation in conversation theory. Grice's theory of dialogue inference is the possibility of providing meaning to the literal. In other words, people apply certain cooperative principles to communicate cooperatively. Thus, conversational implicatures have become one of the top research areas in pragmatics. This paper intends to explore the importance of conversational implicatures in day-to-day conversations in various contexts. It focuses on certain dialogues collected and integrated from the routine conversation. The outcome revealed that context plays a vital role in interpreting utterances. Therefore, there is no possibility of a complete correspondence of one utterance to one context, which shows the conversational implicature cannot be context-independent. In addition, dialogues were classified into Generalized, Scalar, and Particular conversational implicatures.
A Quantitative Study on the Cohesion Problems in English Majors’ Chinese Translation of English Texts
Authors: Li Fengqi , Yao Shanshan
Published: May 2022

Cohesion is an important means to achieve textual coherence. In translation instruction, it is important to raise students’ awareness of textual cohesion in order to improve their translation competence. However, few studies have been conducted to investigate the problems of cohesion that may exist in students’ translated texts. This article compares the cohesive devices used in the Chinese texts translated from English by 38 junior English majors studying in a major university in China with those in a reference Chinese version, for the purpose of revealing the cohesion problems that may exist in the students’ Chinese translation and providing reference for translation instruction. The results show that the major problems include overuse of personal reference and conjunction, especially additive conjunction, insufficient use of ellipsis and repetition which are common cohesive devices in original Chinese texts. The problems reveal, on the one hand, that students may still be under the influence of the source language negative transfer, and on the other hand, that they may have insufficient understanding of Chinese textual cohesion and the differences between English and Chinese in this regard.
Sensorimotor Grounding of Chinese Novel Concepts Constructed From Language Alone
Authors: Zhang Qian , An Xuehua
Published: May 2022

The embodied cognitive view of language asserts that concepts are grounded in sensorimotor experience. In support of this assumption, previous studies have shown that the response times were faster when the movement direction of participants is congruent with the referent position of presented words than that under incongruent condition. This is thought to be evidence that processing these words reactivates sensorimotor experiential traces. Extrapolating from this view, this study aims to explore how concepts without direct experience can be grounded. Participants learned novel concepts related to upward or downward concepts only through a two-sentence description and learned randomly paired novel words. In both experiments, participants judged the sensibility of sentences by upward or downward movements, with the sentences containing novel concepts in Experiment 1 and containing novel words in Experiment 2. Both two experiments found the congruency effect, indicating that when understanding a sentence containing a novel concept or novel word, the sensorimotor experience of the already known concepts in description had been activated automatically, thus realizing the indirect grounding of the novel concept.
Humanizing Satan of Milton’s Paradise Lost
Authors: Dohal Gassim H.
Published: May 2022

Milton wrote an epic in Latin entitled ‘In Quintum Novembris.’ It is written on the occasion of Guy Fawkes' Day. Fawkes and others had plotted to blow up the Parliament House and were discovered in time. According to Nicolson (1963), this “little epic” in Latin “foreshadows Paradise Lost” (p. 77). Probably the man or one of the conspirators of the “little epic” is the Satan of Paradise Lost (PL from now on). Following the actions taken and adopted by Satan in PL, we find that Satan acts like a human being through his deeds and qualities. Anyhow, many critics believe that Milton's Satan is human or humanized at least in some parts in the epic. Not to mention, some critics argue that Satan stands for Milton himself in certain situations. Following his appearance, deeds and qualities, this paper shows how Milton’s Satan of PL is humanized.
Retrasos en el proceso editorial de las publicaciones académicas incluidas en SciELO México
Authors: Marcó del Pont Lalli Raúl , Martínez Navarro Raúl
Published: May 2022

Publicar en revistas de ciencia y tecnología toma generalmente mucho tiempo, lo que puede tener efectos negativos en las carreras académicas (obtención de becas, de plazas y promociones) y en la reputación de las propias publicaciones seriadas. También es una variable relevante que los autores toman en cuenta para decidir dónde publicar. A pesar de los efectos de estos retrasos, existen muy pocos estudios sistemáticos en México sobre el tiempo que le toma a las revistas dar a conocer los textos recibidos. Para cubrir esa ausencia, se analizó este aspecto editorial, a partir de los datos abiertos de SciELO México, y los tiempos que les toma a 196 revistas mexicanas dar a conocer sus artículos, así como las diferencias notables entre publicaciones seriadas de un mismo campo de conocimiento. Se compararon los tiempos resultantes con estudios similares sobre los journals anglosajones, los megajournals y las revistas depredadoras, para ubicar con mayor claridad los procesos editoriales en un entorno global, y vislumbrar las posibilidades de una gestión editorial más eficiente.