bluuebunny/crossref_metadata_2025 huggingface dataset:
Exercise–Linked Irisin: Consequences on Mental and Cardiovascular Health in Type 2 Diabetes
Authors: Sousa Ricardo Augusto Leoni De , Improta-Caria Alex Cleber , Souza Bruno Solano de Freitas
Published: February 2021

Type 2 diabetes mellitus (T2DM) is a metabolic disorder associated with insulin resistance and hyperglycemia. Chronic exposure to a T2DM microenvironment with hyperglycemia, hyperinsulinemia, oxidative stress and increased levels of proinflammatory mediators, has negative consequences to the cardiovascular system and mental health. Therefore, atherosclerotic cardiovascular diseases (CVD) and mental health issues have been strongly associated with T2DM. Lifestyle modifications, including physical exercise training, are necessary to prevent T2DM development and its associated complications. It is widely known that the regular practice of exercise provides several physiological benefits to subjects with T2DM, such as managing glycemic and blood pressure levels. Different types of exercise, from aerobic to resistance training, are effective to improve mental health and cognitive function in T2DM. Irisin is a myokine produced in response to exercise, which has been pointed as a relevant mechanism of action to explain the benefits of exercise on cardiovascular and mental health in T2DM patients. Here, we review emerging clinical and experimental evidence about exercise-linked irisin consequences to cardiovascular and mental health in T2DM.
Differential Effects of STCH and Stress-Inducible Hsp70 on the Stability and Maturation of NKCC2
Authors: Bakhos-Douaihy Dalal , Seaayfan Elie , Demaretz Sylvie , Komhoff Martin , Laghmani Kamel
Published: February 2021

Mutations in the Na-K-2Cl co-transporter NKCC2 lead to type I Bartter syndrome, a life-threatening kidney disease. We previously showed that export from the ER constitutes the limiting step in NKCC2 maturation and cell surface expression. Yet, the molecular mechanisms involved in this process remain obscure. Here, we report the identification of chaperone stress 70 protein (STCH) and the stress-inducible heat shock protein 70 (Hsp70), as two novel binding partners of the ER-resident form of NKCC2. STCH knock-down increased total NKCC2 expression whereas Hsp70 knock-down or its inhibition by YM-01 had the opposite effect. Accordingly, overexpressing of STCH and Hsp70 exerted opposite actions on total protein abundance of NKCC2 and its folding mutants. Cycloheximide chase assay showed that in cells over-expressing STCH, NKCC2 stability and maturation are heavily impaired. In contrast to STCH, Hsp70 co-expression increased NKCC2 maturation. Interestingly, treatment by protein degradation inhibitors revealed that in addition to the proteasome, the ER associated degradation (ERAD) of NKCC2 mediated by STCH, involves also the ER-to-lysosome-associated degradation pathway. In summary, our data are consistent with STCH and Hsp70 having differential and antagonistic effects with regard to NKCC2 biogenesis. These findings may have an impact on our understanding and potential treatment of diseases related to aberrant NKCC2 trafficking and expression.
Therapeutic Targeting of MicroRNAs in the Tumor Microenvironment
Authors: Raue Rebecca , Frank Ann-Christin , Syed Shahzad Nawaz , Brüne Bernhard
Published: February 2021

The tumor-microenvironment (TME) is an amalgamation of various factors derived from malignant cells and infiltrating host cells, including cells of the immune system. One of the important factors of the TME is microRNAs (miRs) that regulate target gene expression at a post transcriptional level. MiRs have been found to be dysregulated in tumor as well as in stromal cells and they emerged as important regulators of tumorigenesis. In fact, miRs regulate almost all hallmarks of cancer, thus making them attractive tools and targets for novel anti-tumoral treatment strategies. Tumor to stroma cell cross-propagation of miRs to regulate protumoral functions has been a salient feature of the TME. MiRs can either act as tumor suppressors or oncogenes (oncomiRs) and both miR mimics as well as miR inhibitors (antimiRs) have been used in preclinical trials to alter cancer and stromal cell phenotypes. Owing to their cascading ability to regulate upstream target genes and their chemical nature, which allows specific pharmacological targeting, miRs are attractive targets for anti-tumor therapy. In this review, we cover a recent update on our understanding of dysregulated miRs in the TME and provide an overview of how these miRs are involved in current cancer-therapeutic approaches from bench to bedside.
Cell-to-Cell Communication by Host-Released Extracellular Vesicles in the Gut: Implications in Health and Disease
Authors: Diaz-Garrido Natalia , Cordero Cecilia , Olivo-Martinez Yenifer , Badia Josefa , Baldomà Laura
Published: February 2021

Communication between cells is crucial to preserve body homeostasis and health. Tightly controlled intercellular dialog is particularly relevant in the gut, where cells of the intestinal mucosa are constantly exposed to millions of microbes that have great impact on intestinal homeostasis by controlling barrier and immune functions. Recent knowledge involves extracellular vesicles (EVs) as mediators of such communication by transferring messenger bioactive molecules including proteins, lipids, and miRNAs between cells and tissues. The specific functions of EVs principally depend on the internal cargo, which upon delivery to target cells trigger signal events that modulate cellular functions. The vesicular cargo is greatly influenced by genetic, pathological, and environmental factors. This finding provides the basis for investigating potential clinical applications of EVs as therapeutic targets or diagnostic biomarkers. Here, we review current knowledge on the biogenesis and cargo composition of EVs in general terms. We then focus the attention to EVs released by cells of the intestinal mucosa and their impact on intestinal homeostasis in health and disease. We specifically highlight their role on epithelial barrier integrity, wound healing of epithelial cells, immunity, and microbiota shaping. Microbiota-derived EVs are not reviewed here.
Chemistry and Toxicology of Major Bioactive Substances in Inocybe Mushrooms
Authors: Patocka Jiri , Wu Ran , Nepovimova Eugenie , Valis Martin , Wu Wenda , Kuca Kamil
Published: February 2021

Mushroom poisoning has always been a threat to human health. There are a large number of reports about ingestion of poisonous mushrooms every year around the world. It attracts the attention of researchers, especially in the aspects of toxin composition, toxic mechanism and toxin application in poisonous mushroom. Inocybe is a large genus of mushrooms and contains toxic substances including muscarine, psilocybin, psilocin, aeruginascin, lectins and baeocystin. In order to prevent and remedy mushroom poisoning, it is significant to clarify the toxic effects and mechanisms of these bioactive substances. In this review article, we summarize the chemistry, most known toxic effects and mechanisms of major toxic substances in Inocybe mushrooms, especially muscarine, psilocybin and psilocin. Their available toxicity data (different species, different administration routes) published formerly are also summarized. In addition, the treatment and medical application of these toxic substances in Inocybe mushrooms are also discussed. We hope that this review will help understanding of the chemistry and toxicology of Inocybe mushrooms as well as the potential clinical application of its bioactive substances to benefit human beings.
Quality Assurance Framework for the Design and Delivery of Virtual, Real-Time Courses
Authors: Karam Marcel , Fares Hanna , Al-Majeed Salah
Published: February 2021

Designing and delivering outcome-based courses that emphasize learner-centric educational discourse and active learning is challenging, especially in online learning environments. Ensuring quality in the design and delivery of such courses in the virtual space requires a well-defined framework with key constituents that interact based on ordered sequences of events. Despite the pressing need for a quality assurance system for today’s virtual, real-time courses, such a system has not been systematically designed. A coherent quality assurance system requires a clear framework that defines the interacting constituents. This work proposes a conceptual and generic “Quality Assurance” (QA) framework, based on experiences primarily in Science, Technology, Engineering, and Mathematics (STEM) fields, for the effective design and delivery of outcome-based virtual, real-time courses that incorporate active learning practices. This Quality Assurance framework may be adjusted to serve as a blueprint that, once adjusted by institutions to accommodate their missions, guides institutions in developing or amending their policies and procedures for the design and delivery of virtual, real-time courses; in addition, such a framework is important for institutions to develop Quality Assurance systems that integrate mechanisms for continuous improvement. The proposed quality assurance framework includes three constituents: a “Teaching and Learning Support” (TLS) that trains educators on pedagogical approaches and the capabilities of the institution’s Learning Management System (LMS); an “Information and Communication Technology Support” (ICTS) that assists educators with the technologies and tools available in the learning management system; and a “Course Management System” (CMS) that encapsulates course design, delivery, and assessment; this study focuses primarily on this “Course Management System” constituent.
Effect of Horizontal Multidirectional Cyclic Loading on Piles in Sand: A Numerical Analysis
Authors: Jenck Orianne , Obaei Armita , Emeriault Fabrice , Dano Christophe
Published: February 2021

Foundations of offshore and nearshore wind energy production systems are subjected to multidirectional and cyclic loads, due to the combined action of wind and waves and in the particular case of mutualized anchor foundations for floating wind turbines, to the phase shift between the loads generated in the adjacent anchored turbines. This article presents a three-dimensional numerical model developed with FLAC3D to analyse the impact of the change in direction of the horizontal load during the cycles. The typical case of a 1.7 m diameter and 10 m-long pile founded in a dense homogeneous sand is considered. A specific procedure has been implemented to apply force-controlled cycles with a change in lateral load direction. The results are compared to mono-directional lateral cyclic loads with the same average and cyclic forces. The results of the parametric study highlight the effect of the average value and amplitude of the cyclic loading on the accumulation of pile head horizontal displacements during the cycles. When a multidirectional cyclic loading is applied, it also leads to an accumulation of the deviated horizontal displacements, and the resulting accumulated horizontal displacements are larger than for a mono-directional cyclic loading of the same amplitude.
Multi-Objective Optimization of Jet Pump Based on RBF Neural Network Model
Authors: Xu Kai , Wang Gang , Zhang Luyao , Wang Liquan , Yun Feihong , Sun Wenhao , Wang Xiangyu , Chen Xi
Published: February 2021

In this study, an annular jet pump optimization method is proposed based on an RBF (Radial Basis Function) neural network model and NSGA-II (Non-Dominated Sorting Genetic Algorithm) optimization algorithm to improve the hydraulic performance of the annular jet pump applied in submarine trenching and dredging. Suction angle, diffusion angle, area ratio and flow ratio were selected as design variables. The computational fluid dynamics (CFD) model was used for numerical simulation to obtain the corresponding performance, and an accurate RBF neural network approximate model was established. Finally, the NSGA-II algorithm was selected to carry out multi-objective optimization and obtain the optimal design variable combination. The results show that the determination coefficient R2 of the two objective functions (jet pump efficiency and head ratio) of the approximate model of the RBF neural network were greater than 0.97. Compared with the original model, the optimized model’s suction angle increased, and the diffusion angle, flow ratio and area ratio decreased. In terms of performance, the head ratio increased by 30.46% after the optimization of the jet pump, and efficiency increased slightly. The proposed jet pump performance optimization method provides a reference for improving the performance of other pumps.
It Is Not Just a Matter of Noise: Sciaena umbra Vocalizes More in the Busiest Areas of the Venice Tidal Inlets
Authors: Picciulin Marta , Facca Chiara , Fiorin Riccardo , Riccato Federico , Zucchetta Matteo , Malavasi Stefano
Published: February 2021

Boat noise is known to have a detrimental effect on a vulnerable Mediterranean sciaenid, the brown meagre Sciaena umbra. During summer 2019, two acoustic surveys were conducted at 40 listening points distributed within the inlet areas of Venice (northern Adriatic Sea). Two five-minute recordings were collected per each point during both the boat traffic hours and the peak of the species’ vocal activity with the aims of (1) characterizing the local noise levels and (2) evaluating the fish spatial distribution by means of its sounds. High underwater broadband noise levels were found (sound pressure levels (SPLs)50–20kHz 107–137 dB re 1 μPa). Interestingly, a significantly higher background noise within the species’ hearing sensibility (100–3150 Hz) was highlighted in the afternoon (113 ± 5 dB re 1 μPa) compared to the night (103 ± 7 dB re 1 μPa) recordings due to a high vessel traffic. A cluster analysis based on Sciaena umbra vocalizations separated the listening points in three groups: highly vocal groups experienced higher vessel presence and higher afternoon noise levels compared to the lower ones. Since the species’ sounds are a proxy of spawning events, this suggests that the reproductive activity was placed in the noisier part of the inlets.
Invasive Fungal Infections after Anti-CD19 Chimeric Antigen Receptor-Modified T-Cell Therapy: State of the Evidence and Future Directions
Authors: Garner Will , Samanta Palash , Haidar Ghady
Published: February 2021

Studies describing invasive fungal infections (IFIs) after chimeric antigen receptor-modified T-cell (CAR-T-cell) therapy are limited. Although post-CAR-T-cell IFIs appear to be uncommon, they are associated with significant morbidity and mortality. Specific risk factors for IFIs in CAR-T-cell recipients have not been fully characterized and are often extrapolated from variables contributing to IFIs in patients with other hematologic malignancies or those undergoing hematopoietic cell transplant. Optimal prophylaxis strategies, including the use of yeast versus mold-active azoles, also remain ill-defined. Further research should investigate key risk factors for IFIs and establish an evidence-based approach to antifungal prophylaxis in these patients in order to improve clinical outcomes.
Paracoccidioidomycosis Diagnosed in Europe—A Systematic Literature Review
Authors: Wagner Gernot , Moertl Deddo , Glechner Anna , Mayr Verena , Klerings Irma , Zachariah Casey , Van den Nest Miriam , Gartlehner Gerald , Willinger Birgit
Published: February 2021

Paracoccidioidomycosis is a systemic mycosis that is endemic in geographical regions of Central and South America. Cases that occur in nonendemic regions of the world are imported through migration and travel. Due to the limited number of cases in Europe, most physicians are not familiar with paracoccidioidomycosis and its close clinical and histopathological resemblance to other infectious and noninfectious disease. To increase awareness of this insidious mycosis, we conducted a systematic review to summarize the evidence on cases diagnosed and reported in Europe. We searched PubMed and Embase to identify cases of paracoccidioidomycosis diagnosed in European countries. In addition, we used Scopus for citation tracking and manually screened bibliographies of relevant articles. We conducted dual abstract and full-text screening of references yielded by our searches. To identify publications published prior to 1985, we used the previously published review by Ajello et al. Overall, we identified 83 cases of paracoccidioidomycosis diagnosed in 11 European countries, published in 68 articles. Age of patients ranged from 24 to 77 years; the majority were male. Time from leaving the endemic region and first occurrence of symptoms considerably varied. Our review illustrates the challenges of considering systemic mycosis in the differential diagnosis of people returning or immigrating to Europe from endemic areas. Travel history is important for diagnostic-workup, though it might be difficult to obtain due to possible long latency period of the disease.
Individualized Hemodynamic Management in Sepsis
Authors: Virág Marcell , Leiner Tamas , Rottler Mate , Ocskay Klementina , Molnar Zsolt
Published: February 2021

Hemodynamic optimization remains the cornerstone of resuscitation in the treatment of sepsis and septic shock. Delay or inadequate management will inevitably lead to hypoperfusion, tissue hypoxia or edema, and fluid overload, leading eventually to multiple organ failure, seriously affecting outcomes. According to a large international survey (FENICE study), physicians frequently use inadequate indices to guide fluid management in intensive care units. Goal-directed and “restrictive” infusion strategies have been recommended by guidelines over “liberal” approaches for several years. Unfortunately, these “fixed regimen” treatment protocols neglect the patient’s individual needs, and what is shown to be beneficial for a given population may not be so for the individual patient. However, applying multimodal, contextualized, and personalized management could potentially overcome this problem. The aim of this review was to give an insight into the pathophysiological rationale and clinical application of this relatively new approach in the hemodynamic management of septic patients.
Differential Interactome Proposes Subtype-Specific Biomarkers and Potential Therapeutics in Renal Cell Carcinomas
Authors: Caliskan Aysegul , Gulfidan Gizem , Sinha Raghu , Arga Kazim Yalcin
Published: February 2021

Although many studies have been conducted on single gene therapies in cancer patients, the reality is that tumor arises from different coordinating protein groups. Unveiling perturbations in protein interactome related to the tumor formation may contribute to the development of effective diagnosis, treatment strategies, and prognosis. In this study, considering the clinical and transcriptome data of three Renal Cell Carcinoma (RCC) subtypes (ccRCC, pRCC, and chRCC) retrieved from The Cancer Genome Atlas (TCGA) and the human protein interactome, the differential protein–protein interactions were identified in each RCC subtype. The approach enabled the identification of differentially interacting proteins (DIPs) indicating prominent changes in their interaction patterns during tumor formation. Further, diagnostic and prognostic performances were generated by taking into account DIP clusters which are specific to the relevant subtypes. Furthermore, considering the mesenchymal epithelial transition (MET) receptor tyrosine kinase (PDB ID: 3DKF) as a potential drug target specific to pRCC, twenty-one lead compounds were identified through virtual screening of ZINC molecules. In this study, we presented remarkable findings in terms of early diagnosis, prognosis, and effective treatment strategies, that deserve further experimental and clinical efforts.
Automatic Feature Selection for Improved Interpretability on Whole Slide Imaging
Authors: Pirovano Antoine , Heuberger Hippolyte , Berlemont Sylvain , Ladjal SaÏd , Bloch Isabelle
Published: February 2021

Deep learning methods are widely used for medical applications to assist medical doctors in their daily routine. While performances reach expert’s level, interpretability (highlighting how and what a trained model learned and why it makes a specific decision) is the next important challenge that deep learning methods need to answer to be fully integrated in the medical field. In this paper, we address the question of interpretability in the context of whole slide images (WSI) classification with the formalization of the design of WSI classification architectures and propose a piece-wise interpretability approach, relying on gradient-based methods, feature visualization and multiple instance learning context. After training two WSI classification architectures on Camelyon-16 WSI dataset, highlighting discriminative features learned, and validating our approach with pathologists, we propose a novel manner of computing interpretability slide-level heat-maps, based on the extracted features, that improves tile-level classification performances. We measure the improvement using the tile-level AUC that we called Localization AUC, and show an improvement of more than 0.2. We also validate our results with a RemOve And Retrain (ROAR) measure. Then, after studying the impact of the number of features used for heat-map computation, we propose a corrective approach, relying on activation colocalization of selected features, that improves the performances and the stability of our proposed method.
Community Detection Problem Based on Polarization Measures: An Application to Twitter: The COVID-19 Case in Spain
Authors: Gutiérrez Inmaculada , Guevara Juan Antonio , Gómez Daniel , Castro Javier , Espínola Rosa
Published: February 2021

In this paper, we address one of the most important topics in the field of Social Networks Analysis: the community detection problem with additional information. That additional information is modeled by a fuzzy measure that represents the risk of polarization. Particularly, we are interested in dealing with the problem of taking into account the polarization of nodes in the community detection problem. Adding this type of information to the community detection problem makes it more realistic, as a community is more likely to be defined if the corresponding elements are willing to maintain a peaceful dialogue. The polarization capacity is modeled by a fuzzy measure based on the JDJpol measure of polarization related to two poles. We also present an efficient algorithm for finding groups whose elements are no polarized. Hereafter, we work in a real case. It is a network obtained from Twitter, concerning the political position against the Spanish government taken by several influential users. We analyze how the partitions obtained change when some additional information related to how polarized that society is, is added to the problem.
Mathematical Representation Competency in Relation to Use of Digital Technology and Task Design—A Literature Review
Authors: Pedersen Mathilde Kjær , Bach Cecilie Carlsen , Gregersen Rikke Maagaard , Højsted Ingi Heinesen , Jankvist Uffe Thomas
Published: February 2021

Representations are crucial to mathematical activity, both for learners and skilled mathematicians. Digital technologies (DT) to support mathematical activity offer a plethora of new possibilities, not least in the context of mathematics education. This paper presents a literature review on representations and activation of students’ representation competency when using DT in mathematics teaching and learning situations. It does so with a starting point in task designs involving digital tools aiming to activate representation competency, drawing on the notion of Mathematical Digital Boundary Object (MDBO). The 30 studies included in the literature review are analyzed using Duval’s registers of semiotic representations and the representation competency from the Danish KOM framework. The results reveal a clear connection between the mathematical topics addressed and the types of representation utilized, and further indicate that certain aspects of the representation competency are outsourced when DT are used. To activate the representation competency in relation to the use of DT, we offer five suggestions for consideration when designing mathematical tasks. Finally, we raise the question of whether DT create new representations or merely new activities.
Flow towards a Stagnation Region of a Vertical Plate in a Hybrid Nanofluid: Assisting and Opposing Flows
Authors: Waini Iskandar , Ishak Anuar , Pop Ioan
Published: February 2021

This study investigates a hybrid nanofluid flow towards a stagnation region of a vertical plate with radiation effects. The hybrid nanofluid consists of copper (Cu) and alumina (Al2O3) nanoparticles which are added into water to form Cu-Al2O3/water nanofluid. The stagnation point flow describes the fluid motion in the stagnation region of a solid surface. In this study, both buoyancy assisting and opposing flows are considered. The similarity equations are obtained using a similarity transformation and numerical results are obtained via the boundary value problem solver (bvp4c) in MATLAB software. Findings discovered that dual solutions exist for both opposing and assisting flows. The heat transfer rate is intensified with the thermal radiation (49.63%) and the hybrid nanoparticles (32.37%).
Focused Review: Potential Rare and Atypical Symptoms as Indicator for Targeted COVID-19 Screening
Authors: Ng Swee Li , Ong Yong Sze , Khaw Kooi Yeong , Teh Siew Phooi , Tan Ching Siang , Ming Long Chiau , Chan Kok-Gan , Lee Learn-Han , Goh Bey-Hing
Published: February 2021

The global pandemic of the coronavirus disease 2019 is a known consequence of infection of severe respiratory syndrome coronavirus-2 (SARS-CoV-2). It has affected nations worldwide with soaring number of cases daily. Symptoms such as fever, cough, and shortness of breath, diarrhea, nausea and vomiting are commonly presented in COVID-19 patients. This focused review aims to discuss these uncommon and atypical COVID-19 symptoms that may be presented which might affect neurological, cardiovascular, cutaneous and ocular systems and their possible mode of actions. Nonetheless, there are some cases of reported uncommon or atypical symptoms which may warrant healthcare professionals to be aware of, especially when in contact with patients. The knowledge and information concerning these symptoms might be able to provide additional cues for healthcare professional by subjecting patients to COVID-19 screening. Meanwhile, it might be able to further enhance the alertness and additional precautions being taken by healthcare personnel, which eventually lead to reduced risk of infections.
Novel Module-Based Membrane Reactor Design Approach for Improved Operability Performance
Authors: Bishop Brent A. , Lima Fernando V.
Published: February 2021

This work aims to address the design and control challenges caused by the integration of phenomena and the loss of degrees of freedom (DOF) that occur in the intensification of membrane reactor units. First, a novel approach to designing membrane reactor units is proposed. This approach consists of designing smaller modules based on specific phenomena such as heat exchange, reactions, and mass transport and combining them in series to produce the final modular membrane-based unit. This approach to designing membrane reactors is then assessed using a process operability analysis for the first time to maximize the operability index, as a way of quantifying the operational performance of intensified processes. This work demonstrates that by designing membrane reactors in this way, the operability of the original membrane reactor design can be significantly improved, translating to an improvement in achievability for a potential control structure implementation.
Low Melting Temperature Sn-Bi Solder: Effect of Alloying and Nanoparticle Addition on the Microstructural, Thermal, Interfacial Bonding, and Mechanical Characteristics
Authors: Kang Hyejun , Rajendran Sri Harini , Jung Jae Pil
Published: February 2021

Sn-based lead-free solders such as Sn-Ag-Cu, Sn-Cu, and Sn-Bi have been used extensively for a long time in the electronic packaging field. Recently, low-temperature Sn-Bi solder alloys attract much attention from industries for flexible printed circuit board (FPCB) applications. Low melting temperatures of Sn-Bi solders avoid warpage wherein printed circuit board and electronic parts deform or deviate from the initial state due to their thermal mismatch during soldering. However, the addition of alloying elements and nanoparticles Sn-Bi solders improves the melting temperature, wettability, microstructure, and mechanical properties. Improving the brittleness of the eutectic Sn-58wt%Bi solder alloy by grain refinement of the Bi-phase becomes a hot topic. In this paper, literature studies about melting temperature, microstructure, inter-metallic thickness, and mechanical properties of Sn-Bi solder alloys upon alloying and nanoparticle addition are reviewed.
Hydrometallurgical Leaching of Copper Flash Furnace Electrostatic Precipitator Dust for the Separation of Copper from Bismuth and Arsenic
Authors: Caplan Michael , Trouba Joseph , Anderson Corby , Wang Shijie
Published: February 2021

Flash furnace electrostatic precipitator dust (FF-ESP dust) is a recycle stream in some primary copper production facilities. This dust contains high amounts of copper. In some cases, the FF-ESP dust contains elevated levels of bismuth and arsenic, both of which cause problems during the electrorefining stages of copper production. Because of this, methods for separation of copper from bismuth and arsenic in FF-ESP dust are necessary. Hydrometallurgical leaching using a number of lixiviants, including sulfuric acid, sulfurous acid, sodium hydroxide, and water, were explored. Pourbaix diagrams of copper, bismuth, and arsenic were used to determine sets of conditions which would thermodynamically separate copper from bismuth and arsenic. The data indicate that water provides the best overall separation between copper and both bismuth and arsenic. Sodium hydroxide provided a separation between copper and arsenic. Sulfurous acid provided a separation between copper and bismuth. Sulfuric acid did not provide any separations between copper and bismuth or copper and arsenic.
Mitochondrial Lipid Signaling and Adaptive Thermogenesis
Authors: Von Bank Helaina , Hurtado-Thiele Mae , Oshimura Nanami , Simcox Judith
Published: February 2021

Thermogenesis is an energy demanding process by which endotherms produce heat to maintain their body temperature in response to cold exposure. Mitochondria in the brown and beige adipocytes play a key role in thermogenesis, as the site for uncoupling protein 1 (UCP1), which allows for the diffusion of protons through the mitochondrial inner membrane to produce heat. To support this energy demanding process, the mitochondria in brown and beige adipocytes increase oxidation of glucose, amino acids, and lipids. This review article explores the various mitochondria-produced and processed lipids that regulate thermogenesis including cardiolipins, free fatty acids, and acylcarnitines. These lipids play a number of roles in thermogenic adipose tissue including structural support of UCP1, transcriptional regulation, fuel source, and activation of cell signaling cascades.
Worms, Fat, and Death: Caenorhabditis elegans Lipid Metabolites Regulate Cell Death
Authors: Perez Marcos A. , Watts Jennifer L.
Published: February 2021

Caenorhabditis elegans is well-known as the model organism used to elucidate the genetic pathways underlying the first described form of regulated cell death, apoptosis. Since then, C. elegans investigations have contributed to the further understanding of lipids in apoptosis, especially the roles of phosphatidylserines and phosphatidylinositols. More recently, studies in C. elegans have shown that dietary polyunsaturated fatty acids can induce the non-apoptotic, iron-dependent form of cell death, ferroptosis. In this review, we examine the roles of various lipids in specific aspects of regulated cell death, emphasizing recent work in C. elegans.
The Autotrophic Core: An Ancient Network of 404 Reactions Converts H2, CO2, and NH3 into Amino Acids, Bases, and Cofactors
Authors: Wimmer Jessica L. E. , Vieira Andrey do Nascimento , Xavier Joana C. , Kleinermanns Karl , Martin William F. , Preiner Martina
Published: February 2021

The metabolism of cells contains evidence reflecting the process by which they arose. Here, we have identified the ancient core of autotrophic metabolism encompassing 404 reactions that comprise the reaction network from H2, CO2, and ammonia (NH3) to amino acids, nucleic acid monomers, and the 19 cofactors required for their synthesis. Water is the most common reactant in the autotrophic core, indicating that the core arose in an aqueous environment. Seventy-seven core reactions involve the hydrolysis of high-energy phosphate bonds, furthermore suggesting the presence of a non-enzymatic and highly exergonic chemical reaction capable of continuously synthesizing activated phosphate bonds. CO2 is the most common carbon-containing compound in the core. An abundance of NADH and NADPH-dependent redox reactions in the autotrophic core, the central role of CO2, and the circumstance that the core’s main products are far more reduced than CO2 indicate that the core arose in a highly reducing environment. The chemical reactions of the autotrophic core suggest that it arose from H2, inorganic carbon, and NH3 in an aqueous environment marked by highly reducing and continuously far from equilibrium conditions. Such conditions are very similar to those found in serpentinizing hydrothermal systems.
The Late Triassic Molasse Deposits in Central Jilin Province, NE China: Constraints on the Paleo-Asian Ocean Closure
Authors: Han Zuozhen , Li Jingjing , Zhu Chenlin , Zhong Wenjian , Song Zhigang
Published: February 2021

This paper presents a new detailed study including zircon U-Pb-Hf isotopic, whole-rock geochemical and Sr-Nd isotopic analyses of conglomerate and granitic pebbles from the molasse deposits in central Jilin Province, NE China. These data are used to better constrain the Late Permian–Triassic tectonic evolution regarding particularly the final closure of the Paleo-Asian Ocean (PAO) along the Changhun-Yanji suture (CYS). Zircon U-Pb data indicate that the granitic pebbles formed in the end-Permian (254–253 Ma). The youngest detrital zircon age of 231 Ma from the conglomerate, and presence of the overlying Upper Triassic Sihetun Formation suggests that the molasse deposits on the Jin′gui Island formed during the Late Triassic. The end-Permian granitic rocks display high SiO2 (66.07–74.77 wt %), with low MgO (0.55–2.05 wt %) and Mg# (31.61–43.64) values, together with depleted Hf and Nd isotopic values (εHf(t) = +1.61 to +11.62; εNd(t) = +3.3 to +4.2; (87Sr/86Sr)i = 0.706458–0.706842) and juvenile second-stage Hf model ages (1148–512 Ma), suggesting that they were probably generated by the partial melting of a Meso-Neoproterozoic juvenile metabasaltic lower crust. They are characterized by enrichments in large ion lithophile elements (LILEs) and depletions in high field strength elements (HFSEs), with affinities to igneous rocks forming in a subduction-related setting. This, combined with regional coeval subduction-related magmatic rocks, indicates that the PAO still existed along the CYS. In addition, the identification of Late Triassic molasse deposits on the Jin′gui Island in this study, coupled with occurrences of many Early Triassic syn-collisional granitoids along the CYS, indicates that the final closure of the PAO took place prior to the Late Triassic.
Experimental Study of Pt Solubility in the CO-CO2 Fluid at Low fO2 and Subsolidus Conditions of the Ultramafic-Mafic Intrusions
Authors: Simakin Alexander , Salova Tamara , Borisova Anastassia Y. , Pokrovski Gleb S. , Shaposhnikova Olga , Tyutyunnik Oksana , Bondarenko Galina , Nekrasov Alexey , Isaenko Sergey I.
Published: February 2021

The solubility of Pt in CO-CO2 fluid was studied experimentally at P = 50–200 MPa and T = 950 °C. A mixture of MgC2O4 and MgCO3 was used as a source of the fluid. Upon the reaction of the Pt capsule walls and the fluid, a carbonyl of platinum is formed. The use of the high-temperature quartz ceramics as a fluid trap avoids the effect of mechanical contamination with Pt from the eroded capsule walls. The total content of platinum in the porous fluid traps was measured by the Electrothermal Atomic Absorption (ET-AAS) method. In some experiments, the local analysis of traps was carried out by the Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) method. The composition of fluid in bubbles captured in an albite glass trap was studied by micro-Raman spectroscopy. On the capsule walls and MgO, Pt “whiskers” of submicron diameter were observed, which were formed as a product of carbonyl decomposition during quenching. About 5–15% of carbonyl withstands quenching resulting in 1.5 to 2 ppm Pt soluble in acetone (runs at P = 200 MPa) in a quartz glass trap. The amount of Pt soluble in acetone from the capsule walls corresponds to a concentration of up to 8 ppm in the fluid. A high content of soluble Pt of 2000–3000 ppm was determined in a carbon coated MgO matrix. Our study demonstrated that the solubility of Pt in the CO-CO2 fluid is 15–150 ppm, presumably in the form of Pt3(CO)62− under conditions corresponding to the conditions of the subsolidus stage of layered ultramafic-mafic and ultramafic-alkaline intrusions formation. Our preliminary data showed that this solubility will increase with the addition of water at low fO2.
Re-Os Age and Stable Isotope (O-H-S-Cu) Geochemistry of North Eastern Turkey’s Kuroko-Type Volcanogenic Massive Sulfide Deposits: An Example from Cerattepe-Artvin
Authors: Ucurum Ali , Demir Cigdem Sahin , Otlu Nazmi , Erturk Mustafa , Ekici Taner , Kirk Jason , Ruiz Joaquin , Mathur Ryan , Arehart Greg B.
Published: February 2021

The eastern Pontide tectonic belt (EPTB) contains greater than 350 identified Kuroko type volcanogenic massive sulfide deposits/mineralization/occurrences (VMSD). The deposits are associated with Late Cretaceous felsic volcanics consisting mainly of dacitic and rhyolitic lavas and pyroclastics that outcrop within a narrow zone running parallel to the eastern Black Sea coast and represent the axial zone of a paleo-magmatic arc. The Cerattepe deposit is the second-largest and is a hybrid VMS system with some epithermal features. To date, no geochemical research constrains the origin and timing of mineralization in the Cerattepe VMS deposit. Here, we provide Cu, O, H and S, isotope analysis of ores and alteration minerals to understand the hydrothermal history of the deposit and date the massive ore with Re-Os geochronology. Secondary weathering mobilized and redistributed metals in the deposit. The copper isotope signatures of shallow ores in the gossan follow patterns resulting from oxidative weathering of copper minerals with gossan Fe oxides of δ65Cu = −2.59‰, enrichment zone copper sulfide of d65Cu = +2.23 and +1.73‰, and primary ores of δ65Cu = +0.71 and +0.41‰. At the boundary of the enrichment zone, further cycling and migration of enrichment zone copper are evidenced by two samples having larger ranges of the δ65Cu = +3.59‰, and −2.93‰. Evidence for a magmatic source for fluids and S are evidenced by the O and H isotope values from quartz veins (δ18O = +7.93‰ to +10.82‰, and δD = −78‰ and −68‰) and sulfides that possess δ34S ratios of –5 and 0‰ from drill core samples. 187Os/188Os–187Re/188Os ratios from drill core sulfide samples of Cerattepe VMS deposit yields a 62±3 Ma isochron age and a highly radiogenic Os initial ratio. This age is compatible with silicate alteration ages from a proximal deposit and clearly shows mineralization occurs at a much younger time than previously proposed for VMS mineralization in the eastern Pontides. The new Re-Os age and source of Os imply that mineralization in the area occurs at a distinctly younger interval in the back-arc basin and metals could be sourced from the surrounding host rocks.
Scale-Up of Decanter Centrifuges for the Particle Separation and Mechanical Dewatering in the Minerals Processing Industry by Means of a Numerical Process Model
Authors: Menesklou Philipp , Sinn Tabea , Nirschl Hermann , Gleiss Marco
Published: February 2021

Decanter centrifuges are frequently used for thickening, dewatering, classification, or degritting in the mining industry and various other sectors. Their use in an industrial process chain requires a sufficiently accurate prediction of the product and the machine behaviour. For this purpose, experiments on a smaller pilot-scale are carried out for scale-up of a decanter centrifuge, which is usually a major challenge. Predicting the process behaviour of decanter centrifuges from laboratory tests is rather difficult. Basically, there are two common ways of scale-up: First, via analytical methods and the law of similarity, which often requires an enormous experimental effort. Second, using numerical models, which demands a mathematically and physically precise description of the multiple processes running simultaneously in such machines. This article provides an overview of both methods for scale-up of a decanter centrifuge. The concept of a previous developed numerical approach is introduced. Pros and cons of both scale-up methods are compared and further discussed. Experiments on lab-scale, pilot-scale, and industrial-scale decanter centrifuges with two different finely dispersed calcium carbonate water suspensions were carried out and simulations were done to investigate and prove the scale-up capability and transferability of the numerical approach.
Electronic Properties of Triangle Molybdenum Disulfide (MoS2) Clusters with Different Sizes and Edges
Authors: Wang Songsong , Han Changliang , Ye Liuqi , Zhang Guiling , Hu Yangyang , Li Weiqi , Jiang Yongyuan
Published: February 2021

The electronic structures and transition properties of three types of triangle MoS2 clusters, A (Mo edge passivated with two S atoms), B (Mo edge passivated with one S atom), and C (S edge) have been explored using quantum chemistry methods. The highest occupied molecular orbital (HOMO)–lowest unoccupied molecular orbital (LUMO) gap of B and C is larger than that of A, due to the absence of the dangling of edge S atoms. The frontier orbitals (FMOs) of A can be divided into two categories, edge states from S3p at the edge and hybrid states of Mo4d and S3p covering the whole cluster. Due to edge/corner states appearing in the FMOs of triangle MoS2 clusters, their absorption spectra show unique characteristics along with the edge structure and size.
The Effects of New Zealand Grown Ginseng Fractions on Cytokine Production from Human Monocytic THP-1 Cells
Authors: Chen Wei , Balan Prabhu , Popovich David G.
Published: February 2021

Pro-inflammatory cytokines and anti-inflammatory cytokines are important mediators that regulate the inflammatory response in inflammation-related diseases. The aim of this study is to evaluate different New Zealand (NZ)-grown ginseng fractions on the productions of pro-inflammatory and anti-inflammatory cytokines in human monocytic THP-1 cells. Four NZ-grown ginseng fractions, including total ginseng extract (TGE), non-ginsenoside fraction extract (NGE), high-polar ginsenoside fraction extract (HPG), and less-polar ginsenoside fraction extract (LPG), were prepared and the ginsenoside compositions of extracts were analyzed by HPLC using 19 ginsenoside reference standards. The THP-1 cells were pre-treated with different concentrations of TGE, NGE, HPG, and LPG, and were then stimulated with lipopolysaccharide (LPS). The levels of pro-inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and anti-inflammatory cytokines, such as interleukin-10 (IL-10), and transforming growth factor beta-1 (TGF-β1), were determined by enzyme-linked immunosorbent assay (ELISA). TGE at 400 µg/mL significantly inhibited LPS-induced TNF-α and IL-6 productions. NGE did not show any effects on inflammatory secretion except inhibited IL-6 production at a high dose. Furthermore, LPG displayed a stronger effect than HPG on inhibiting pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) productions. Particularly, 100 µg/mL LPG not only significantly inhibited the production of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, but also remarkably enhanced the production of anti-inflammatory cytokine IL-10. NZ-grown ginseng exhibited anti-inflammatory effects in vitro, which is mainly attributed to ginsenoside fractions (particularly less-polar ginsenosides) rather than non-saponin fractions.
Organic Contaminants and Interactions with Micro- and Nano-Plastics in the Aqueous Environment: Review of Analytical Methods
Authors: Reichel Julia , Graßmann Johanna , Knoop Oliver , Drewes Jörg E. , Letzel Thomas
Published: February 2021

Micro- and nanoplastic particles are increasingly seen not only as contaminants themselves, but also as potential vectors for trace organic chemicals (TOrCs) that might sorb onto these particles. An analysis of the sorbed TOrCs can either be performed directly from the particle or TOrCs can be extracted from the particle with a solvent. Another possibility is to analyze the remaining concentration in the aqueous phase by a differential approach. In this review, the focus is on analytical methods that are suitable for identifying and quantifying sorbed TOrCs on micro- and nano-plastics. Specific gas chromatography (GC), liquid chromatography (LC) and ultraviolet-visible spectroscopy (UV-VIS) methods are considered. The respective advantages of each method are explained in detail. In addition, influencing factors for sorption in the first place are being discussed including particle size and shape (especially micro and nanoparticles) and the type of polymer, as well as methods for determining sorption kinetics. Since the particles are not present in the environment in a virgin state, the influence of aging on sorption is also considered.
Ferroelectric Particles in Nematic Liquid Crystals with Soft Anchoring
Authors: Cirtoaje Cristina
Published: February 2021

A theoretical evaluation of the electric Freedericksz transition threshold and saturation field is proposed for a liquid crystals composite with ferroelectric particles. Existing models consider a strong anchoring of nematic molecules on the glass support of the cell, but in this paper a soft molecular anchoring of molecules on the glass support and also on the ferroelectric nanoparticle’s surface is assumed. Thus, a finite saturation field was obtained in agreement with real systems. Calculations are made for planar configuration of positive dielectric anisotropy liquid crystals. The results are compared with data obtained on similar systems from different publications and the differences are discussed.
The Antiproliferative and Apoptotic Effect of a Novel Synthesized S-Triazine Dipeptide Series, and Toxicity Screening in Zebrafish Embryos
Authors: Malebari Azizah M. , Abd Alhameed Rakia , Almarhoon Zainab , Farooq Muhammad , Wadaan Mohammad A. M. , Sharma Anamika , de la Torre Beatriz G. , Albericio Fernando , El-Faham Ayman
Published: February 2021

Several derivatives containing morpholine/piperidine, anilines, and dipeptides as pending moieties were prepared using s-triazine as a scaffold. These compounds were evaluated for their anticancer activity against two human breast cancer cell lines (MCF-7 and MDA-MB-231), a colon cancer cell line (HCT-116), and a non-tumorigenic cell line (HEK 293). Tamoxifen was used as a reference. Animal toxicity tests were carried out in zebrafish embryos. Most of these compounds showed a higher activity against breast cancer than colon cancer. Compound 3a—which contains morpholine, aniline, and glycylglycinate methyl ester—showed a high level of cytotoxicity against MCF-7 cells with IC50 values of less than 1 µM. This compound showed a much lower level of toxicity against the non-tumorigenic HEK-293 cell line, and in the in vivo studies using zebrafish embryos. Furthermore, it induced cell cycle arrest at the G2/M phase, and apoptosis in MCF-7 cells. On the basis of our results, 3a emerges as a potential candidate for further development as a therapeutic drug to treat hormone receptor-positive breast cancer.