bluuebunny/crossref_metadata_2025 huggingface dataset:
Autonomous Driving System Architecture with Integrated ROS2 and Adaptive AUTOSAR
Authors: Hong Dongwon , Moon Changjoo
Published: March 2024

In the automotive industry, research is now underway to apply Adaptive Automotive Open System Architecture (AUTOSAR) to the development of next-generation mobility, such as autonomous driving and connected cars. However, research on autonomous driving is being predominantly conducted on the robotics platform ROS2 (Robot Operating System 2). This demonstrates a considerable distance between autonomous driving research and its application in actual vehicles. To bridge this gap, interoperability that leverages the strengths of the Adaptive AUTOSAR and ROS2 platforms and compensates for their weaknesses is required. Therefore, this study proposes an architecture for interoperability between the two platforms, named Autonomous Driving System with Integrated ROS2 and Adaptive AUTOSAR (ASIRA). The proposed architecture enables communication between each of the two platforms through the ROS2 SOME/IP Bridge and allows for the necessary data exchange. It validates them in autonomous driving scenarios and goes beyond vehicle development, testing, and prototyping to exploit the advantages of each platform. Additionally, the simulation of autonomous vehicles within the ASIRA architecture is demonstrated by interoperating the ROS2 representative open-source autonomous driving project, Autoware, with the Adaptive AUTOSAR simulator. This study contributes to the assimilation of ROS2 into the automotive industry and its application in real vehicles by linking ROS2 and Adaptive AUTOSAR.
A Map-Aided Fast Initialization Method for the Magnetic Positioning of Vehicles
Authors: Lu Yi , Wei Dongyan , Li Wen , Ji Xinchun , Yuan Hong
Published: March 2024

Magnetic positioning is a promising technique for vehicles in global navigation satellite system (GNSS)-denied scenarios. In general, a fixed-length magnetic sequence is required to provide an initial positioning result, which means that users need to wait a relatively long distance. To minimize this initialization distance, a map-aided fast initialization method, including magnetic database construction and magnetic positioning, is proposed in this paper. For magnetic database construction, a multisource fused database is established using a precise and effective strategy in which the positions of reference points (RPs) and the diverse information of paths are obtained from the map and the magnetic field is calculated using data collected during driving. For magnetic positioning, we innovatively propose a coarse–fine combination method that improves the positioning accuracy within a short distance. In the coarse map matching stage, by detecting the vehicle’s motion and utilizing the topological relationships between paths, the search range is precisely narrowed. In the fine magnetic localization stage, an improved mean absolute difference (MAD) metric and a derivative metric are combined to form a joint matching criterion to determine the positioning result. The experimental results illustrate the importance of each module in the proposed method, which improves the precision of the database up to 80% and significantly shortens the initialization distance up to 50%.
Greenspace Exposure with Chronic Obstructive Pulmonary Disease: A Systematic Review
Authors: Gao Yijie , Pan Kunhao , Li Hongyi , Zhao Bing
Published: March 2024

Although a growing number of studies have suggested the relevance of greenspace exposure to respiratory health, most have explored single greenfield indicators or selected disease outcomes. This systematic review aimed to comprehensively assess the evidence that greenspace exposure is associated with chronic obstructive pulmonary disease (COPD) incidence, prevalence, mortality, and related lung function indicators and to explore the impact of greenspace exposure on COPD-related disease outcomes. We searched the literature published before 28 September 2023, using six electronic databases and snowball searches. After screening 3689 studies, 16 eligible studies were included. These studies examined various greenspace indicators such as the Normalized Difference Vegetation Index (NDVI), canopy coverage, greenspace coverage, and land cover conditions. Most studies have reported the positive effects of greenspace exposure. However, the findings had significant heterogeneity, with large differences in the greenness assessment, COPD identification methods, and population characteristics. The mechanisms of this impact have not been conclusively determined, but we propose several hypotheses to explain the pathways by which greenspace exposure affects COPD. Overall, our findings contribute valuable knowledge for understanding the relationship between greenspace exposure and COPD, informing future research in this field.
Effects of Thinning on Carbon Storage in a Mixed Broadleaved Plantation in a Subtropical Area of China
Authors: Lin Na , Feng Mingchun , Huang Huanqiang , Qiu Zhanpeng , Ma Tao , Chen Shiqing
Published: March 2024

Forest thinning is a widely used silvicultural method in forest management and has complex effects on carbon sequestration in different types of forest ecosystems. The present study examined the short-term effects of different thinning intensities on carbon storage in an 11-year-old mixed broadleaved plantation. The results partially supported that different thinning intensities have varying impacts on carbon storage in different parts of forest ecosystems. The main results were as follows: (1) The effect of thinning on promoting the growth of fast-growing tree species (Michelia macclurei Dandy and Schima superba Gardn. et Champ.) was earlier than that of slow-growing tree species (Castanopsis hystrix Miq.). (2) A greater thinning intensity conferred greater effects on promoting the tree biomass carbon growth, litter carbon storage, and understory plant diversity, in the order of 41%~50% > 31%~40% > 20%~30%, but these values were lower than those for the unthinned plots. (3) The soil carbon storage declined most in the 41%~50% thinned plots, due to the reduced carbon storage in the humus layer. (4) The 20%~30% thinning intensity promoted carbon sequestration in the short term in the mixed broadleaved plantation. The results suggested that a lower thinning intensity promoted carbon sequestration in the short term, a greater thinning intensity reduced carbon storage at first, but the negative effect on carbon storage exhibited trade-offs later by the growth of tree and understory plant biomass carbon and the accumulation of litter layer carbon.
Spatial Pattern of Genetic Diversity and Demographic History Revealed by Population Genomic Analysis: Resilience to Climate Fluctuations of Acer truncatum Bunge
Authors: Liao Jia , Yu Xuedan , Wu Yuxia , Pei Shunxiang , Xin Xuebing , Xia Xinhe , Mao Shan , Pan Xinyue , Zheng Yongqi , Zhang Chuanhong
Published: March 2024

Whole genome sequencing techniques are capable of providing insights into plant genetic adaptation to climate oscillations. Acer truncatum Bunge is a new resource tree for food with ornamental, timber and medicinal value. However, it has been listed as a near-threatened species because of the decreasing number of natural populations. In order to develop conservation strategies and sustainable innovative utilization for genetic resources, spatial pattern of genetic diversity and demographic history of A. truncatum from 13 natural populations, which cover the entire range, were analyzed by 9,086,353 single nucleotide polymorphisms (SNPs) through whole genome resequencing. The genetic diversity of natural populations was high (He = 0.289, I = 0.449), and genetic variations mainly resided within populations (82.47%) by AMOVA analysis. Cluster analysis showed that 13 natural populations mainly originated from three common gene pools. Therefore, it is recommended that the natural populations (LBGM, BTM, WLS and DQT) with high genetic diversity in different groups were given priority for in situ conservation and the genetic resources from these populations were collected for ex situ conservation. The effective population size of A. truncatum experienced two large-scale sharp contractions and currently decreased to its bottom in history. Nonetheless, A. truncatum could have expanded its effective population size to its historical peak after the last glacial period, indicating that it is highly resilient to fluctuations of temperature and humidity.
Driving Forces and Ecological Restoration Revelation in Southwest China Based on the Divergence Characteristics of Ecosystem Compound Use Efficiency
Authors: Wang Yuxi , Peng Li , Chen Tiantian , Yu Pujia , Zhang Junyi , Xia Chengcheng
Published: March 2024

Ecosystem carbon use efficiency (CUE), water use efficiency (WUE), and light use efficiency (LUE) are critical parameters for estimating CO2 uptake, water circulation, and ecosystem balance. Research on the change trends of individual use efficiency has matured; however, studies on the spatiotemporal heterogeneity and driving mechanisms of divergence patterns for multi-use efficiencies capability are limited. Therefore, taking southwest China as an example, this study constructed a compound use efficiency (COM) through CUE, WUE, and LUE. Based on the spatiotemporal patterns and divergence characteristics analysis of water–carbon–light use efficiencies, the scale effects and driving mechanism of its divergence characteristics for COM at the optimal scale were clarified. The results revealed that the average value of CUE, LUE, WUE, and COM were 0.49, 0.7 gC m−2 MJ−1, 2.31 gC kg−1 H2O, and 0.87, respectively. Apart from CUE, the LUE, WUE, and COM parameters exhibited a fluctuating upward trend. Statistically, there was a high COM in karst and ecological restoration regions, reflecting the strong adaptability of karst vegetation and the effectiveness of ecological restoration; as the elevation rose, COM increased and then decreased, with the highest value at the elevation of 3000 m; the lowest COM was found in grassland, refuting the inference that it can be used as an optimal vegetation type for China’s Grain to Green program from the perspective of use efficiency. Sub-basin was the most optimal divergence scale, and although temperature and elevation were the dominant single force causing COM divergence, the couplings of precipitation and population density and elevation and population density had more controlling impacts than a single force. These findings enrich the understanding of ecosystem use efficiency and are beneficial for the improvement in ecological restoration strategies in karst landscapes.
Continuous Space Wireless Communication Tower Placement by Hybrid Simulated Annealing
Authors: Tang Maolin , Li Wei
Published: March 2024

Wireless communication tower placement arises in many real-world applications. This paper investigates a new emerging wireless communication tower placement problem, namely, continuous space wireless communication tower placement. Unlike existing wireless communication tower placement problems, which are discrete computational problems, this new wireless communication tower placement problem is a continuous space computational problem. In this paper, we formulate the new wireless communication tower placement problem and propose a hybrid simulated annealing algorithm that can take advantage of the powerful exploration capacity of simulated annealing and the strong exploitation capacity of a local optimization procedure. We also demonstrate through experiments the effectiveness of this hybridization technique and the good performance and scalability of the hybrid simulated annulling in this paper.
Exploring the Role of Extracellular Vesicles in the Pathogenesis of Tuberculosis
Authors: Arya Rakesh , Shakya Hemlata , Chaurasia Reetika , Haque Md Azizul , Kim Jong-Joo
Published: March 2024

Tuberculosis (TB) remains a significant global health concern, necessitating accurate diagnosis and treatment monitoring. Extracellular vesicles (EVs), including exosomes, play crucial roles in disease progression, with their associated genes serving as potential biomarkers and therapeutic targets. Leveraging publicly available RNA-Seq datasets of TB patients and healthy controls (HCs), to identify differentially expressed genes (DEGs) and their associated protein–protein interaction networks and immune cell profiles, the common EV-related DEGs were identified and validated in the GSE42830 and GSE40553 datasets. We have identified nine common EV-related DEGs (SERPINA1, TNFAIP6, MAPK14, STAT1, ITGA2B, VAMP5, CTSL, CEACAM1, and PLAUR) upregulated in TB patients. Immune cell infiltration analysis revealed significant differences between TB patients and HCs, highlighting increased proportions of various immune cells in TB patients. These DEGs are involved in crucial cellular processes and pathways related to exocytosis and immune response regulation. Notably, VAMP5 exhibited excellent diagnostic performance (AUC—0.993, sensitivity—93.8%, specificity—100%), with potential as a novel biomarker for TB. The EV-related genes can serve as novel potential biomarkers that can distinguish between TB and HCs. VAMP5, which functions in exosome biogenesis and showed significant upregulation in TB, can be targeted for therapeutic interventions and treatment outcomes.
Genome-Wide Analysis of the LBD Gene Family in Melon and Expression Analysis in Response to Wilt Disease Infection
Authors: Zheng Ling , Chao Yanrong , Wang Yian , Xu Yizhuo , Li Shipeng
Published: March 2024

LBD transcription factors are a class of transcription factors that regulate the formation of lateral organs, establish boundaries, and control secondary metabolism in plants. In this study, we identified 37 melon LBD transcription factors using bioinformatics methods and analyzed their basic information, chromosomal location, collinearity, evolutionary tree, gene structure, and expression patterns. The results showed that the genes were unevenly distributed across the 13 chromosomes of melon plants, with tandem repeats appearing on chromosomes 11 and 12. These 37 transcription factors can be divided into two major categories, Class I and Class II, and seven subfamilies: Ia, Ib, Ic, Id, Ie, IIa, and IIb. Of the 37 included transcription factors, 25 genes each contained between one to three introns, while the other 12 genes did not contain introns. Through cis-acting element analysis, we identified response elements such as salicylic acid, MeJA, abscisic acid, and auxin, gibberellic acid, as well as light response, stress response, and MYB-specific binding sites. Expression pattern analysis showed that genes in the IIb subfamilies play important roles in the growth and development of various organs in melon plants. Expression analysis found that the majority of melon LBD genes were significantly upregulated after infection with wilt disease, with the strongest response observed in the stem.
Attention toward Social and Non-Social Stimuli in Preschool Children with Autism Spectrum Disorder: A Paired Preference Eye-Tracking Study
Authors: Congiu Sara , Doneddu Giuseppe , Fadda Roberta
Published: March 2024

Different dimensions of visual attention to social (human faces) and non-social stimuli (objects) were assessed in 19 preschool children with Autism Spectrum Disorder (ASD) and 19 typically developing (TD) age, gender, and IQ-matched controls through an original paired preference eye-tracking paradigm. The present study found a significantly reduced attentional bias toward human faces in children with ASD compared to TD controls. The analysis of the total fixation time showed a significantly reduced preference for faces in children with ASD compared to TD children. Moreover, while TD children showed a significant preference for the face over the object, children in the ASD group observed the two paired pictures for a similar amount of time, thus showing no preference. Besides, children with ASD paid significantly more sustained attention to the objects than TD children. Children in the TD group paid greater sustained attention to the faces over the objects, while children in the ASD group did not differentiate between objects and faces. Finally, an age effect was found in ASD, as younger children in the group tended to prefer objects and to show more sustained attention towards them. Overall, these findings add to the literature on anomalies in attention toward social and non-social stimuli in young children with ASD compared to their TD counterparts. These results are discussed in the light of previous studies and suggest possible directions for future research.
Comparative Analysis of Single-Cell RNA Sequencing Methods with and without Sample Multiplexing
Authors: Xie Yi , Chen Huimei , Chellamuthu Vasuki Ranjani , Lajam Ahmad bin Mohamed , Albani Salvatore , Low Andrea Hsiu Ling , Petretto Enrico , Behmoaras Jacques
Published: March 2024

Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful technique for investigating biological heterogeneity at the single-cell level in human systems and model organisms. Recent advances in scRNA-seq have enabled the pooling of cells from multiple samples into single libraries, thereby increasing sample throughput while reducing technical batch effects, library preparation time, and the overall cost. However, a comparative analysis of scRNA-seq methods with and without sample multiplexing is lacking. In this study, we benchmarked methods from two representative platforms: Parse Biosciences (Parse; with sample multiplexing) and 10x Genomics (10x; without sample multiplexing). By using peripheral blood mononuclear cells (PBMCs) obtained from two healthy individuals, we demonstrate that demultiplexed scRNA-seq data obtained from Parse showed similar cell type frequencies compared to 10x data where samples were not multiplexed. Despite relatively lower cell capture affecting library preparation, Parse can detect rare cell types (e.g., plasmablasts and dendritic cells) which is likely due to its relatively higher sensitivity in gene detection. Moreover, a comparative analysis of transcript quantification between the two platforms revealed platform-specific distributions of gene length and GC content. These results offer guidance for researchers in designing high-throughput scRNA-seq studies.
An Overview on the Big Players in Bone Tissue Engineering: Biomaterials, Scaffolds and Cells
Authors: Ferraz Maria Pia
Published: March 2024

Presently, millions worldwide suffer from degenerative and inflammatory bone and joint issues, comprising roughly half of chronic ailments in those over 50, leading to prolonged discomfort and physical limitations. These conditions become more prevalent with age and lifestyle factors, escalating due to the growing elderly populace. Addressing these challenges often entails surgical interventions utilizing implants or bone grafts, though these treatments may entail complications such as pain and tissue death at donor sites for grafts, along with immune rejection. To surmount these challenges, tissue engineering has emerged as a promising avenue for bone injury repair and reconstruction. It involves the use of different biomaterials and the development of three-dimensional porous matrices and scaffolds, alongside osteoprogenitor cells and growth factors to stimulate natural tissue regeneration. This review compiles methodologies that can be used to develop biomaterials that are important in bone tissue replacement and regeneration. Biomaterials for orthopedic implants, several scaffold types and production methods, as well as techniques to assess biomaterials’ suitability for human use—both in laboratory settings and within living organisms—are discussed. Even though researchers have had some success, there is still room for improvements in their processing techniques, especially the ones that make scaffolds mechanically stronger without weakening their biological characteristics. Bone tissue engineering is therefore a promising area due to the rise in bone-related injuries.
A Stem-like Patient-Derived Ovarian Cancer Model of Platinum Resistance Reveals Dissociation of Stemness and Resistance
Authors: Suzuki Tise , Conant Ashlyn , Jung Yeonkyu , Bax Ryan , Antonissen Ashley , Chen Wanqiu , Yu Gary , Ioffe Yevgeniya J. , Wang Charles , Unternaehrer Juli J.
Published: March 2024

To understand chemoresistance in the context of cancer stem cells (CSC), a cisplatin resistance model was developed using a high-grade serous ovarian cancer patient-derived, cisplatin-sensitive sample, PDX4. As a molecular subtype-specific stem-like cell line, PDX4 was selected for its representative features, including its histopathological and BRCA2 mutation status, and exposed to cisplatin in vitro. In the cisplatin-resistant cells, transcriptomics were carried out, and cell morphology, protein expression, and functional status were characterized. Additionally, potential signaling pathways involved in cisplatin resistance were explored. Our findings reveal the presence of distinct molecular signatures and phenotypic changes in cisplatin-resistant PDX4 compared to their sensitive counterparts. Surprisingly, we observed that chemoresistance was not inherently linked with increased stemness. In fact, although resistant cells expressed a combination of EMT and stemness markers, functional assays revealed that they were less proliferative, migratory, and clonogenic–features indicative of an underlying complex mechanism for cell survival. Furthermore, DNA damage tolerance and cellular stress management pathways were enriched. This novel, syngeneic model provides a valuable platform for investigating the underlying mechanisms of cisplatin resistance in a clinically relevant context, contributing to the development of targeted therapies tailored to combat resistance in stem-like ovarian cancer.
Huntington’s Disease: Complex Pathogenesis and Therapeutic Strategies
Authors: Tong Huichun , Yang Tianqi , Xu Shuying , Li Xinhui , Liu Li , Zhou Gongke , Yang Sitong , Yin Shurui , Li Xiao-Jiang , Li Shihua
Published: March 2024

Huntington’s disease (HD) arises from the abnormal expansion of CAG repeats in the huntingtin gene (HTT), resulting in the production of the mutant huntingtin protein (mHTT) with a polyglutamine stretch in its N-terminus. The pathogenic mechanisms underlying HD are complex and not yet fully elucidated. However, mHTT forms aggregates and accumulates abnormally in neuronal nuclei and processes, leading to disruptions in multiple cellular functions. Although there is currently no effective curative treatment for HD, significant progress has been made in developing various therapeutic strategies to treat HD. In addition to drugs targeting the neuronal toxicity of mHTT, gene therapy approaches that aim to reduce the expression of the mutant HTT gene hold great promise for effective HD therapy. This review provides an overview of current HD treatments, discusses different therapeutic strategies, and aims to facilitate future therapeutic advancements in the field.
What Is the “Hydrogen Bond”? A QFT-QED Perspective
Authors: Renati Paolo , Madl Pierre
Published: March 2024

In this paper we would like to highlight the problems of conceiving the “Hydrogen Bond” (HB) as a real short-range, directional, electrostatic, attractive interaction and to reframe its nature through the non-approximated view of condensed matter offered by a Quantum Electro-Dynamic (QED) perspective. We focus our attention on water, as the paramount case to show the effectiveness of this 40-year-old theoretical background, which represents water as a two-fluid system (where one of the two phases is coherent). The HB turns out to be the result of the electromagnetic field gradient in the coherent phase of water, whose vacuum level is lower than in the non-coherent (gas-like) fraction. In this way, the HB can be properly considered, i.e., no longer as a “dipolar force” between molecules, but as the phenomenological effect of their collective thermodynamic tendency to occupy a lower ground state, compatible with temperature and pressure. This perspective allows to explain many “anomalous” behaviours of water and to understand why the calculated energy associated with the HB should change when considering two molecules (water-dimer), or the liquid state, or the different types of ice. The appearance of a condensed, liquid, phase at room temperature is indeed the consequence of the boson condensation as described in the context of spontaneous symmetry breaking (SSB). For a more realistic and authentic description of water, condensed matter and living systems, the transition from a still semi-classical Quantum Mechanical (QM) view in the first quantization to a Quantum Field Theory (QFT) view embedded in the second quantization is advocated.
Targeting Vascular Impairment, Neuroinflammation, and Oxidative Stress Dynamics with Whole-Body Cryotherapy in Multiple Sclerosis Treatment
Authors: Dziedzic Angela , Maciak Karina , Miller Elżbieta Dorota , Starosta Michał , Saluk Joanna
Published: March 2024

Multiple sclerosis (MS), traditionally perceived as a neurodegenerative disease, exhibits significant vascular alternations, including blood–brain barrier (BBB) disruption, which may predispose patients to increased cardiovascular risks. This vascular dysfunction is intricately linked with the infiltration of immune cells into the central nervous system (CNS), which plays a significant role in perpetuating neuroinflammation. Additionally, oxidative stress serves not only as a byproduct of inflammatory processes but also as an active contributor to neural damage. The synthesis of these multifaceted aspects highlights the importance of understanding their cumulative impact on MS progression. This review reveals that the triad of vascular damage, chronic inflammation, and oxidative imbalance may be considered interdependent processes that exacerbate each other, underscoring the need for holistic and multi-targeted therapeutic approaches in MS management. There is a necessity for reevaluating MS treatment strategies to encompass these overlapping pathologies, offering insights for future research and potential therapeutic interventions. Whole-body cryotherapy (WBCT) emerges as one of the potential avenues for holistic MS management approaches which may alleviate the triad of MS progression factors in multiple ways.
Co-Expression Network Analysis Unveiled lncRNA-mRNA Links Correlated to Epidermal Growth Factor Receptor-Tyrosine Kinase Inhibitor Resistance and/or Intermediate Epithelial-to-Mesenchymal Transition Phenotypes in a Human Non-Small Cell Lung Cancer Cellular Model System
Authors: Fustaino Valentina , Papoff Giuliana , Ruberti Francesca , Ruberti Giovina
Published: March 2024

We investigated mRNA-lncRNA co-expression patterns in a cellular model system of non-small cell lung cancer (NSCLC) sensitive and resistant to the epithelial growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) erlotinib/gefitinib. The aim of this study was to unveil insights into the complex mechanisms of NSCLC targeted therapy resistance and epithelial-to-mesenchymal transition (EMT). Genome-wide RNA expression was quantified for weighted gene co-expression network analysis (WGCNA) to correlate the expression levels of mRNAs and lncRNAs. Functional enrichment analysis and identification of lncRNAs were conducted on modules associated with the EGFR-TKI response and/or intermediate EMT phenotypes. We constructed lncRNA-mRNA co-expression networks and identified key modules and their enriched biological functions. Processes enriched in the selected modules included RHO (A, B, C) GTPase and regulatory signaling pathways, apoptosis, inflammatory and interleukin signaling pathways, cell adhesion, cell migration, cell and extracellular matrix organization, metabolism, and lipid metabolism. Interestingly, several lncRNAs, already shown to be dysregulated in cancer, are connected to a small number of mRNAs, and several lncRNAs are interlinked with each other in the co-expression network.
The Formation and Function of the VTA Dopamine System
Authors: Hou Guoqiang , Hao Mei , Duan Jiawen , Han Ming-Hu
Published: March 2024

The midbrain dopamine system is a sophisticated hub that integrates diverse inputs to control multiple physiological functions, including locomotion, motivation, cognition, reward, as well as maternal and reproductive behaviors. Dopamine is a neurotransmitter that binds to G-protein-coupled receptors. Dopamine also works together with other neurotransmitters and various neuropeptides to maintain the balance of synaptic functions. The dysfunction of the dopamine system leads to several conditions, including Parkinson’s disease, Huntington’s disease, major depression, schizophrenia, and drug addiction. The ventral tegmental area (VTA) has been identified as an important relay nucleus that modulates homeostatic plasticity in the midbrain dopamine system. Due to the complexity of synaptic transmissions and input–output connections in the VTA, the structure and function of this crucial brain region are still not fully understood. In this review article, we mainly focus on the cell types, neurotransmitters, neuropeptides, ion channels, receptors, and neural circuits of the VTA dopamine system, with the hope of obtaining new insight into the formation and function of this vital brain region.
Helicobacter pylori Eradication Reverses DNA Damage Response Pathway but Not Senescence in Human Gastric Epithelium
Authors: Kalisperati Polyxeni , Spanou Evangelia , Pateras Ioannis S. , Evangelou Konstantinos , Thymara Irene , Korkolopoulou Penelope , Kotsinas Athanassios , Vlachoyiannopoulos Panayiotis G. , Tzioufas Athanasios G. , Kanellopoulos Christos , Gorgoulis Vassilis G. , Sougioultzis Stavros
Published: March 2024

Helicobacter pylori (H. pylori) infection induces DNA Double-Strand Breaks (DSBs) and consequently activates the DNA Damage Response pathway (DDR) and senescence in gastric epithelium. We studied DDR activation and senescence before and after the eradication of the pathogen. Gastric antral and corpus biopsies of 61 patients with H. pylori infection, prior to and after eradication treatment, were analyzed by means of immunohistochemistry/immunofluorescence for DDR marker (γH2AΧ, phosporylated ataxia telangiectasia-mutated (pATM), p53-binding protein (53BP1) and p53) expression. Samples were also evaluated for Ki67 (proliferation index), cleaved caspase-3 (apoptotic index) and GL13 staining (cellular senescence). Ten H. pylori (−) dyspeptic patients served as controls. All patients were re-endoscoped in 72-1361 days (mean value 434 days), and tissue samples were processed in the same manner. The eradication of the microorganism, in human gastric mucosa, downregulates γH2AΧ expression in both the antrum and corpus (p = 0.00019 and p = 0.00081 respectively). The expression of pATM, p53 and 53BP1 is also reduced after eradication. Proliferation and apoptotic indices were reduced, albeit not significantly, after pathogen clearance. Moreover, cellular senescence is increased in H. pylori-infected mucosa and remains unaffected after eradication. Interestingly, senescence was statistically increased in areas of intestinal metaplasia (IM) compared with adjacent non-metaplastic mucosa (p < 0.001). In conclusion, H. pylori infection triggers DSBs, DDR and senescence in the gastric epithelium. Pathogen eradication reverses the DDR activation but not senescence. Increased senescent cells may favor IM persistence, thus potentially contributing to gastric carcinogenesis.
Management and Outcomes of Very Long-Chain Acyl-CoA Dehydrogenase Deficiency (VLCAD Deficiency): A Retrospective Chart Review
Authors: Al Bandari Maria , Nagy Laura , Cruz Vivian , Hewson Stacy , Hossain Alomgir , Inbar-Feigenberg Michal
Published: March 2024

Very long-chain acyl-CoA dehydrogenase (VLCAD) deficiency is a rare genetic condition affecting the mitochondrial beta-oxidation of long-chain fatty acids. This study reports on the clinical outcomes of patients diagnosed by newborn screening with VLCAD deficiency comparing metabolic parameters, enzyme activities, molecular results, and clinical management. It is a single-center retrospective chart review of VLCAD deficiency patients who met the inclusion criteria between January 2002 and February 2020. The study included 12 patients, 7 of whom had an enzyme activity of more than 10%, and 5 patients had an enzyme activity of less than 10%. The Pearson correlation between enzyme activity and the C14:1 level at newborn screening showed a p-value of 0.0003, and the correlation between enzyme activity and the C14:1 level at diagnosis had a p-value of 0.0295. There was no clear correlation between the number of documented admissions and the enzyme activity level. Patients who had a high C14:1 value at diagnosis were started on a diet with a lower percentage of energy from long-chain triglycerides. The C14:1 result at diagnosis is the value that has been guiding our initial clinical management in asymptomatic diagnosed newborns. However, the newborn screening C14:1 value is the most sensitive predictor of low enzyme activity and may help guide dietary management.
A Study of Learning Environment for Initiating Flutter App Development Using Docker
Authors: Aung Soe Thandar , Funabiki Nobuo , Aung Lynn Htet , Kinari Safira Adine , Mentari Mustika , Wai Khaing Hsu
Published: March 2024

The Flutter framework with Dart programming allows developers to effortlessly build applications for both web and mobile from a single codebase. It enables efficient conversions to native codes for mobile apps and optimized JavaScript for web browsers. Since utilizing a wide range of widgets in Flutter ensures consistent experiences on various devices for users, it becomes crucial in programming education by providing a unified environment for learning app development while reducing the need for platform-specific knowledge. However, the setup of the Flutter environment is challenging for novice students due to its multiple steps, such as installing dependencies and configuring environments. To support independent learning for these students, it is essential to simplify the setup by providing user-friendly instructions and automated tools. In this paper, we present a Docker-based environment for Flutter app developments across Windows, Linux, and Mac through Visual Studio Code, ensuring a unified learning experience. This paper aims to simplify complex configurations and address the obstacles encountered by students when initiating Flutter projects. For the evaluation, we prepared three simple Flutter projects along with the setup environment in a Docker container. Then, we asked 24 Master’s students at Okayama University, Japan, to install the environment and modify the source codes in the projects independently by following the given instructions. The results show that all the students successfully completed the assignments, which confirms the efficiency and validity of our proposal.
Changes in the Range of Four Advantageous Grasshopper Habitats in the Hexi Corridor under Future Climate Conditions
Authors: Li Donghong , Gan Huilin , Li Xiaopeng , Zhou Huili , Zhang Hang , Liu Yaomeng , Dong Rui , Hua Limin , Hu Guixin
Published: March 2024

Angaracris rhodopa (Fischer et Walheim), Calliptamus abbreviatus (Ikonnikov), Myrmeleotettix palpalis (Zubowsky), and Oedaleus decorus asiaticus (Bey-Bienko) are the main grasshoppers that harm the natural grassland in the Hexi Corridor in Gansu, northwest China. In this study, the MaxEnt model was employed to identify the key environmental factors affecting the distribution of the four grasshoppers’ habitats and to assess their distribution under current and future climate conditions. The aim was to provide a basis for grasshopper monitoring, prediction, and precise control. In this study, distribution of suitable habitats for A. rhodopa, C. abbreviates, M. palpalis, O. decorus asiaticus were predicted under current and future climatic scenarios using the Maxent model. The average AUC (area under the ROC curve) and TSS (true skill statistic) values of the four grasshoppers were greater than 0.9, and the simulation results were excellent and highly reliable. The mean annual precipitation was the main factor limiting the current range of suitable areas for these four species. Under the current climate, A. rhodopa, C. abbreviatus, and O. decorus asiaticus were mainly distributed in the central and eastern parts of the Hexi Corridor, and M. palpalis was distributed throughout the Hexi Corridor, with a suitable area of 1.29 × 104, 1.43 × 104, 1.44 × 104, and 2.12 × 104 km2, accounting for 13.7%, 15.2%, 15.3%, and 22.5% of the total area of the grasslands in the Hexi Corridor, respectively. The highly suitable areas of A. rhodopa, C. abbreviatus, and O. decorus asiaticus were mainly distributed in the eastern-central part of Zhangye City, the western part of Wuwei City, and the western and southern parts of Jinchang City, with areas of 0.20 × 104, 0.29 × 104, and 0.35 × 104 km2, accounting for 2.2%, 3%, and 3.7% of the grassland area, respectively. The high habitat of M. palpalis was mainly distributed in the southeast of Jiuquan City, the west, middle, and east of Zhangye City, the west of Wuwei City, and the west and south of Jinchang City, with an area of 0.32 × 104 km2, accounting for 3.4% of the grassland area. In the 2030s, the range of A. rhodopa, C. abbreviatus, and O. decorus asiaticus was predicted to increase; the range of M. palpalis will decrease. The results of this study could provide a theoretical basis for the precise monitoring and control of key areas of grasshoppers in the Hexi Corridor.
The Stability of Social and Behavioral Rhythms and Unexpected Low Rate of Relevant Depressive Symptoms in Old Adults during the COVID-19 Pandemic
Authors: Sancassiani Federica , Cossu Giulia , Cantone Elisa , Romano Ferdinando , Perra Alessandra , Urban Antonio , Pinna Samantha , Del Giacco Stefano , Littera Roberto , Firinu Davide , Chessa Luchino , Tramontano Enzo , Nardi Antonio Egidio , Carta Mauro Giovanni
Published: March 2024

Background: The disruption of social rhythms was found to be associated with depressive disorders during the COVID-19 pandemic; lower rates of these disorders were surprisingly found in old adults. The present study aims to verify the stability of social rhythms during lockdown in a sample of elderly people. Methods: Controlled cohort study (secondary analyses) of a previous randomized-controlled trial with the first evaluation in April 2019 (T0) and then 48 weeks later (T1) during the lockdown. The regulation of social and behavioral rhythms was measured through the Brief Social Rhythms Scale (BSRS); the Patient Health Questionnaire-9 (PHQ9) was adopted to detect relevant depressive symptoms. Results: 93 elderlies (73.36 ± 4.97 years old, 50.5% females) were evaluated at T0 and T1. Neither the total score of BSRS nor any of the 10 items showed a statistically significant difference comparing the two survey periods. The frequency of relevant depressive symptoms was 5.3% at T0 and 6.4% at T1 (OR = 0.8, CI95% 0.2–24). Conclusions: Among elderlies who did not show an increased risk of depression during the lockdown, social and behavioral rhythms remained exceptionally stable during the same period. Considering previous evidence about rhythms dysregulation preceding depression, their stability may be considered a factor of resilience.
The Use of Glenoid Structural Allografts for Glenoid Bone Defects in Reverse Shoulder Arthroplasty
Authors: Ingoe Helen , Italia Kristine , Gilliland Luke , Kang Hean Wu , Karel Mirek , Maharaj Jashint , Cutbush Kenneth , Gupta Ashish
Published: March 2024

Background: The use of reverse shoulder arthroplasty as a primary and revision implant is increasing. Advances in implant design and preoperative surgical planning allow the management of complex glenoid defects. As the demand for treating severe bone loss increases, custom allograft composites are needed to match the premorbid anatomy. Baseplate composite structural allografts are used in patients with eccentric and centric defects to restore the glenoid joint line. Preserving bone stock is important in younger patients where a revision surgery is expected. The aim of this article is to present the assessment, planning, and indications of femoral head allografting for bony defects of the glenoid. Methods: The preoperative surgical planning and the surgical technique to execute the plan with a baseplate composite graft are detailed. The preliminary clinical and radiological results of 29 shoulders which have undergone this graft planning and surgical technique are discussed. Clinical outcomes included visual analogue score of pain (VAS), American Shoulder and Elbow Surgeons score (ASES), Constant–Murley score (CS), satisfaction before and after operation, and active range of motion. Radiological outcomes included graft healing and presence of osteolysis or loosening. Results: The use of composite grafts in this series has shown excellent clinical outcomes, with an overall graft complication rate in complex bone loss cases of 8%. Conclusion: Femoral head structural allografting is a valid and viable surgical option for glenoid bone defects in reverse shoulder arthroplasty.
Frequency, Severity, Risk Factors, and Outcome of Hemorrhagic Transformation in Anterior and Posterior Stroke
Authors: Ayub Tanya , Barwari Awini , Finsterer Josef
Published: March 2024

Background: There are few data on hemorrhagic transformation in posterior circulation strokes (PCS) compared to anterior circulation strokes (ACS). The aim of this study was to retrospectively analyze the incidence of hemorrhagic transformation, its different subtypes, the associations with different risk factors, and the outcome of ACS and PCS patients. Methods: A retrospective analysis of consecutive ischemic stroke patients with hemorrhagic transformation was performed. Clinical and demographic data were collected from electronic patient records. Results: Included were 186 ACS patients and 67 PCS patients. The median age was 77 years, with PCS patients being slightly younger than ACS patients. ACS patients were more likely to be treated with acetylsalicylic acid before stroke. ACS and PCS patients had comparable frequencies and severity of hemorrhagic transformation. After excluding ACS patients who received thrombectomy, PCS patients developed hemorrhagic transformation more frequently compared to ACS patients. Risk factors for hemorrhagic transformation did not differ between ACS and PCS patients and included vitamin K antagonist use before stroke and thrombectomy in ACS patients. There was no correlation between hemorrhagic transformation and stroke outcome. Conclusions: Hemorrhagic transformation occurs with similar frequency in PCS and ACS patients but is more common in PCS patients after the exclusion of ACS patients undergoing thrombectomy.
Posterior Shoulder Instability and Glenoid Bone Loss: A Review and a Free Bone Graft Technique
Authors: Smith Walter Ryan , Edwards T. Bradley
Published: March 2024

Posterior glenoid bone loss (pGBL) is frequently associated with posterior shoulder instability. Posterior glenohumeral instability accounts for a small percentage of shoulder pathologies, and critical bone loss in posterior instability has not been well defined in the literature. Younger patient populations who participate in activities that repetitively stress the posterior stabilizing structures of the shoulder are more prone to developing posterior shoulder instability. A variety of surgical options have been described, ranging from isolated capsulolabral repair to glenoid osteotomy. Soft-tissue repair alone may be an inadequate treatment in cases of pGBL and places patients at a high risk of recurrence. Our preferred technique for posterior glenoid reconstruction in cases of pGBL involves the transfer of a free iliac crest bone graft onto the native glenoid. The graft is contoured to fit the osseous defect and secured to provide an extension of the glenoid track. In this study, we review pGBL in the setting of posterior instability and describe our technique in detail. Further long-term studies are needed to refine the indications for glenoid bone graft procedures and quantify what constitutes a critical pGBL.
Retinal Microvasculature in Systemic Sclerosis Patients and the Correlation between Nailfold Capillaroscopic Findings and Optical Coherence Angiography Results
Authors: Paczwa Katarzyna , Rerych Magdalena , Romanowska-Próchnicka Katarzyna , Olesińska Marzena , Różycki Radosław , Gołębiewska Joanna
Published: March 2024

Background: The comparison of retinal perfusion in the eyes of patients with systemic sclerosis (SSc) and in healthy controls using optical coherence tomography angiography (OCTA). The correlation between nailfold capillaroscopy results and OCTA findings among SSc. Methods: The study enrolled 31 patients with systemic sclerosis and 41 healthy controls. OCTA was performed in both groups to assess the retinal vasculature in the superficial (SCP) and deep (DCP) capillary plexuses and the foveal avascular zone (FAZ) area. Nailfold capillaroscopy (NC) was performed in SSc patients and compared to the FAZ area and the superficial and the deep vessel density. Results: In the SSc group, the parafoveal vessel density in DCP was significantly higher in relation to the mean value (p < 0.0001) and in each quadrant of the macula (p < 0.0001) compared to healthy subjects (p < 0.0001). The patients with early scleroderma patterns in capillaroscopy had a larger superficial and deep FAZ (p = 0.0104, p = 0.0076, respectively) than those with active and late patterns. There was a statistically significant difference in the FAZ when comparing early to active (p < 0.0001) and early to late scleroderma patterns (p < 0.0001). A statistically significant difference was found in the type of interstitial lung disease and the deep FAZ area (p = 0.0484). SSc patients with nonspecific interstitial pneumonia (NSIP) had a larger FAZ than those with usual interstitial pneumonia (UIP) (p = 0.0484). Moreover, NSIP cases had a higher parafoveal mean superficial vessel density than those with UIP (p = 0.0471). Conclusions: Our investigation showed that the peripheral microvascular system correlates with ocular microcirculatory impairment. The results indicate the important role of OCTA in the diagnosis, monitoring, and prognosis of microvascular changes in SSc.
Assessment of the Cyclic Fatigue Performance of the Novel Protaper Ultimate File System Used in Different Kinematics: An In Vitro Study
Authors: Diaconu Cezar Tiberiu , Diaconu Anca Elena , Tuculina Mihaela Jana , Mihai Laurența Lelia , Gheorghiță Mircea , Gheorghiță Lelia Mihaela , Mărășescu Petre , Gliga Alexandru , Diaconu Oana Andreea
Published: March 2024

This in vitro study aims to assess the cyclic fatigue resistance of the Protaper Ultimate (PTU) files compared to the Protaper Gold (PTG) and the M3 UDG (M3) files using various motion kinematics in simulated canals. As far as the authors are aware, no study has previously compared the three file systems before this current investigation. Therefore, closing this information gap is the goal of the current research. Methods: A total of (60 new endodontic files were randomly divided into 6 groups (10 files per group); groups 1, 3, and 5 used continuous rotation (CR), while groups 2, 4, and 6 used forward reciprocating motion (FRM). A manufactured stainless-steel artificial canal was used to perform the cyclic fatigue testing. The results were analyzed using Student’s t-test and two-way ANOVA. All pairwise comparisons revealed statistically significant differences in the time to failure (TTF) for every study group (p < 0.001), with the exception of the PTG and M3 files, which performed similarly using both CR and FRM. Conclusions: The PTU files performed better than the PTG and M3 files in terms of the TTF and number of cycles to failure (NCF) using both CR and FRM.