bluuebunny/crossref_metadata_2025 huggingface dataset:
The impact of feed efficiency selection on the ruminal, cecal, and fecal microbiomes of Angus steers from a commercial feedlot
Authors: Welch Christina B , Lourenco Jeferson M , Davis Dylan B , Krause Taylor R , Carmichael Mia N , Rothrock Michael J , Pringle T Dean , Callaway Todd R
Published: July 2020

Feed is the greatest cost of animal production, so reducing it is critical to increase producer profits. In ruminants, the microbial population within the gastrointestinal tract (GIT) is critical to nutrient digestion and absorption in both the rumen and the hindgut. The objective of this study was to determine the bacterial taxonomic profile of the rumen, cecum, and feces of feedlot steers at slaughter in order to link feed efficiency and the GIT bacterial populations from these three locations. Twenty commercial Angus steers were selected and divided into two groups according to their residual feed intake (RFI) classification determined during the feedlot-finishing period: high-RFI (n = 10) and low-RFI (n = 10). After the ruminal, cecal, and fecal samples were collected at slaughter, DNA extraction and 16S rRNA gene sequencing were performed on them to determine their bacterial composition. One-way ANOVA was performed on the animal performance data, alpha diversities, and bacterial abundances using RFI classification as the fixed effect. Overall, the ruminal bacterial population was the most different in terms of taxonomic profile compared with the cecal and fecal populations as revealed by beta diversity analysis (P < 0.001). Moreover, bacterial richness (Chao1) was greatest (P = 0.01) in the rumen of the high-RFI group compared with the low-RFI group. In contrast, bacterial richness and diversity in the intestinal environment showed that Chao1 was greater (P = 0.01) in the cecum, and the Shannon diversity index was greater in both the cecum and feces of low-RFI compared with high-RFI steers (P = 0.01 and P < 0.001, respectively). Ruminococcaceae was more abundant in the low-RFI group in the cecum and feces (P = 0.01); fecal Bifidobacteriaceae was more abundant in high-RFI steers (P = 0.03). No correlations (P ≥ 0.13) between any ruminal bacterial family and RFI were detected; however, Ruminococcaceae, Mogibacteriaceae, Christensenellaceae, and BS11 were negatively correlated with RFI (P < 0.05) in the cecum and feces. Succinivibrionaceae in the cecum was positively correlated with RFI (P = 0.05), and fecal Bifidobacteriaceae was positively correlated with RFI (P = 0.03). Results collectively indicate that in addition to the ruminal bacteria, the lower gut bacterial population has a significant impact on feed efficiency and nutrient utilization in feedlot steers; therefore, the intestinal bacteria should also be considered when examining the basis of ruminant feed efficiency.
A 6-Year Experience of Laser Treatments for Burn Scars in a Regional Burn Center—Safety, Efficacy, and Quality Improvement
Authors: Zhang Christopher Q , Gogal Christina , Gaugler Trent , Blome-Eberwein Sigrid
Published: July 2020

Laser treatments have long been used as a treatment method for burn scars. Since 2012, more than 1800 laser treatments were performed at Lehigh Valley Health Network Burn Center, far exceeding any previous cohort in studies exploring laser treatments for burn scars. Although previous research has looked at improving scar appearance and physiology with laser treatments, very few have focused on safety. The purpose of the study was to determine whether laser treatments are a safe treatment option for burn scars. Four hundred and fourteen patients who had undergone at least one laser treatment in the outpatient burn center since 2012 were analyzed. Electronic medical records (EPIC) were reviewed. The data were entered in REDCap and later exported to Microsoft Excel and R Studio for statistical analysis. Most of the complications found were related to the moderate sedation during the procedures and were mild, ie, nausea. The most common adverse effect was prolonged recovery time, which can affect practice flow. The overall postoperative complication rate for laser treatments with and without moderate sedation was minimal at 2.2% and 1.4%, respectively. Pain during and after the procedure averaged 3.9 and 1.7, respectively, on a 1 to 10 scale. The Vancouver Scar Scale showed modest improvement in scar appearance over time with an average improvement of 1.4. The high variability of the Vancouver Scar Scale observed in this series underlines its lack of sensitivity. The study results show that laser treatments for burn scars in the outpatient setting generally are safe for patients in need of burn scar intervention. Some practice flow adjustments need to be taken into consideration when offering these procedures in an outpatient setting.
Emergency surgery for acute cholecystitis associated with cholecystocolonic fistula caused by gallbladder cancer: a case report
Authors: Sunakawa Taiki , Ko Kenju , Moriyasu Ryo , Nishida Yasunori , Otagiri Noriaki , Tauchi Katsunori
Published: July 2020

Preoperative diagnosis of cholecystocolonic fistula (CCF) is difficult and the contribution of gallbladder cancer or colon cancer is unclear when there is associated malignancy. We present a case that was diagnosed with acute cholecystitis associated with CCF by multidetector computed tomography (MDCT) preoperatively and malignant neoplasm during emergency surgery. She was finally diagnosed with gallbladder cancer after the operation and underwent a two-stage surgery for regional lymph node dissection. Gallbladder cancer can be a primary malignant cancer causing CCF, whereas MDCT is useful for preoperative diagnosis of CCF. A treatment plan in consideration of gallbladder cancer is advisable for CCF associated with malignant tumor.
The impact of strong recombination on temperature determination in planetary nebulae
Authors: Gómez-Llanos V , Morisset C , García-Rojas J , Jones D , Wesson R , Corradi R L M , Boffin H M J
Published: July 2020

The long-standing difference in chemical abundances determined from optical recombination lines and collisionally excited lines raises questions about our understanding of atomic physics, as well as the assumptions made when determining physical conditions and chemical abundances in astrophysical nebulae. Here, we study the recombination contribution of [O iii] 4363 and the validity of the line ratio [O iii] 4363/4959 as a temperature diagnostic in planetary nebulae with a high abundance discrepancy. We derive a fit for the recombination coefficient of [O iii] 4363 that takes into account the radiative and dielectronic recombinations, for electron temperatures from 200 to 30 000 K. We estimate the recombination contribution of [O iii] 4363 for the planetary nebulae Abell 46 and NGC 6778 by subtracting the collisional contribution from the total observed flux. We find that the spatial distribution for the estimated recombination contribution in [O iii] 4363 follows that of the O ii 4649 recombination line, both peaking in the central regions of the nebula, especially in the case of Abell 46 that has a much higher abundance discrepancy. The estimated recombination contribution reaches up to 70 and 40 per cent of the total [O iii] 4363 observed flux, for Abell 46 and NGC 6778, respectively.
Urinary excretion of epidermal growth factor and rapid loss of kidney function
Authors: Norvik Jon Viljar , Harskamp Laura R , Nair Viji , Shedden Kerby , Solbu Marit D , Eriksen Bjørn O , Kretzler Matthias , Gansevoort Ron T , Ju Wenjun , Melsom Toralf
Published: July 2020

Background

Lower urinary excretion of the kidney tubule–specific biomarker epidermal growth factor (uEGF) is associated with increased risk of renal function [glomerular filtration rate (GFR)] loss in diabetes and in patients with established chronic kidney disease (CKD). We investigated whether uEGF is associated with rapid GFR decline or incident CKD in the general population.

Methods

Subjects without CKD or diabetes were recruited from the general population in Tromso, Norway [Renal Iohexol Clearance Survey (RENIS); N = 1249] and Groningen, the Netherlands [Prevention of REnal and Vascular END-stage disease (PREVEND); N = 4534], with a median follow-up of 5.6 and 7.4 years, respectively. GFR was measured by iohexol clearance in the RENIS and estimated using the CKD Epidemiology Collaboration creatinine–cystatin C equation in the PREVEND study. Rapid GFR decline was defined as an annual GFR loss >3.0 mL/min/1.73 m2 and in sensitivity analyses as subjects with the 10% steepest GFR slope within each cohort.

Results

Lower baseline uEGF excretion was associated with rapid GFR loss in both cohorts {RENIS, odds ratio [OR] per 1 μg/mmol lower uEGF 1.42 [95% confidence interval (CI) 1.06–1.91], P = 0.02; PREVEND, OR 1.29 [95% CI 1.10–1.53], P < 0.01}, adjusted for baseline GFR, albumin:creatinine ratio and conventional CKD risk factors. Similar results were obtained using the outcome of the 10% steepest GFR slope in each cohort. Lower uEGF levels were associated with incident CKD in the combined analysis of both cohorts.

Conclusions

Lower uEGF levels are associated with increased risk of rapid GFR loss and incident CKD in the general population. This finding, together with previous findings in CKD and high-risk populations, supports that uEGF may serve as a broadly applicable biomarker representing the tubular component of the current glomerulus-centric clinical risk assessment system.
Increasing Drug Resistance Among Persons With Tuberculosis in Massachusetts, 2009–2018
Authors: Eddy Jared J , Gadani Kavita M , Tibbs Andrew , Bernardo John , Cochran Jennifer , White Laura F , Horsburgh C Robert , Jacobson Karen R
Published: July 2020

We examined Massachusetts tuberculosis surveillance data from to 2009 to 2018. Of 1533 culture-confirmed cases, 190 (12.4%) demonstrated resistance to isoniazid including 32 (2.1%) with rifampin resistance. In multivariable analysis, isoniazid resistance increased significantly over time (per-year odds ratio = 1.07, 95% confidence interval = 1.01–1.13, P = .018) and was associated with younger age, foreign birth, and prior tuberculosis treatment.
Antibiotics and Innate Immunity: A Cooperative Effort Towards the Successful Treatment of Infections
Authors: Berti Andrew , Rose Warren , Nizet Victor , Sakoulas George
Published: July 2020

Despite the common ancestry of antimicrobial and immunological science, a divergence driven by artificially construed paradigms in microbiology has placed limits on how we understand the mechanisms of antibiotics in vivo. We summarize recent updates on data that shed light on how antibiotics interact with components of innate immunity.
World Health Organization growth standards: How do Canadian children measure up?
Authors: Rodd Celia , Feely Allison , Becker Allan B , Moraes Theo J , Subbarao Padmaja , Mandhane Piushkumar J , Turvey Stuart E , Lefebvre Diana L , Sears Malcolm R , Azad Meghan B , Sharma Atul
Published: July 2020

Background

World Health Organization (WHO) growth standards for children aged 0 to 5 years describe growth under optimal conditions and were adopted for use in Canada in 2012. We are seeking to validate these charts in a well-characterized, longitudinal cohort of healthy, Canadian youngsters, assess tracking over time, and evaluate the prognostic implications of early growth.

Methods

Data from 2,795 mother–infant dyads from the CHILD birth cohort were classified by feeding modality at 6 months as exclusively breastfed, partially breastfed, or formula-fed. WHO z-scores (z) were calculated at birth, 3 months, 1 year, and 3 years. Receiver operator characteristics (ROC) assessed the predictive performance of early weight (WT), weight-for-length (WfL), or body mass index (BMI) z-scores for overweight/obesity at 3 years.

Results

Compared to WHO standards, Canadian children at birth had lower median WfLz (−0.73) and BMIz (−0.29), with more positive scores by 3 years (WfLz=BMIz=0.58). At both 1 and 3 years, formula feeding was associated with higher scores than breastfeeding, even after regression adjustment for covariates. Head circumference z-score was typically positive at all times and regardless of feeding modality. At 1 year, ROC area under the curve was 0.79 for WTz, WfLz, and BMIz, and BMIz>0.88 identified children with increased risk of overweight/obesity (BMIz >2) at age 3 years (20.3% versus 3.0%, P<0.001).

Conclusions

Compared to WHO growth charts, Canadian children at 3 years show an upward shift in BMIz and WfLz, particularly when formula-fed. Infant growth parameters may identify infants with increased risk of overweight/obesity at age 3 years; early recognition may allow targeting infants at higher risk.
Functional Characterization of Terpene Synthases Accounting for the Volatilized-Terpene Heterogeneity in Lathyrus odoratus Cultivar Flowers
Authors: Bao Tingting , Shadrack Kimani , Yang Song , Xue Xinxin , Li Shuying , Wang Ning , Wang Qiuyue , Wang Li , Gao Xiang , Cronk Quentin
Published: July 2020

Lathyrus odoratus (sweet pea) is an ornamental plant with exceptional floral scent, previously used as an experimental organism in the early development of Mendelian genetics. However, its terpene synthases (TPSs), which act as metabolic gatekeepers in the biosynthesis of volatile terpenoids, remain to be characterized. Auto-Headspace Solid-phase Microextraction/Gas chromatography–mass spectrometry analysis of floral volatile terpene constituents from seven sweet pea cultivars identified α-bergamotene, linalool, (−)-α-cubebene, geraniol, β-caryophyllene and β-sesquiphellandrene as the dominant compounds. RNA sequencing was performed to profile the transcriptome of L. odoratus flowers. Bioinformatic analysis identified eight TPS genes (acronymed as LoTPS) that were successfully cloned, heterologously expressed and functionally analyzed. LoTPS4 and LoTPS7, belonging to the TPS-b clade, biochemically catalyzed the formation of monoterpenes and sesquiterpenes. LoTPS3 and LoTPS8, placed in the TPS-a clade, also generated monoterpenes and sesquiterpenes, while LoTPS12 belonging to the TPS-g clade showed linalool/nerolidol synthase activity. Notably, biochemical assays of the recombinant LoTPS proteins revealed their catalytic promiscuity, and the enzymatic products were basically consistent with major volatile compounds released from sweet pea flowers. The data from our study lay the foundation for the chemical ecology, molecular genetics and biotechnological improvement of sweet pea and other legumes (Fabaceae).
Identifying Predictors of Recommendations for and Participation in Multimodal Nonpharmacological Treatments for Chronic Pain Using Patient-Reported Outcomes and Electronic Medical Records
Authors: Bernstein Cheryl , Gillman Andrea G , Zhang Di , Bartman Anna E , Jeong Jong-Hyeon , Wasan Ajay D
Published: July 2020

Objective

High-quality chronic pain care emphasizes multimodal treatments that include medication and nonpharmacological treatments. But it is not clear which patients will participate in nonpharmacological treatments, such as physical therapy or mental health care, and previous research has shown conflicting evidence.

Methods

We used the Patient Outcomes Repository for Treatment (PORT) registry, which combines patient-reported outcomes data with electronic medical records. In this retrospective observational study, we performed two separate multinomial regression analyses with feature selection to identify PORT variables that were predictive of 1) recommendation of a nonpharmacological treatment by the provider and 2) patient participation in nonpharmacological treatments. Two hundred thirty-six patients were recommended (REC) or not recommended (NO REC) a nonpharmacological treatment, and all REC patients were classified as participating (YES) or not participating (NO) in the recommendations.

Results

Female gender and a diagnosis of Z79 “Opioid drug therapy” were significant positive and negative predictors of nonpharmacological treatment recommendations, respectively. Schedule II opioid use at initial presentation and recommendations for rehabilitation therapy were significant predictors of nonparticipation.

Conclusions

Patients using opioids are less likely to be recommended nonpharmacological treatments as part of multimodal chronic pain care and are less likely to participate in nonpharmacological treatments once recommended. Males are also less likely to be recommended nonpharmacological treatments. Patients referred for rehabilitation therapies are less likely to comply with those recommendations. We have identified patients in vulnerable subgroups who may require additional resources and/or encouragement to comply with multimodal chronic pain treatment recommendations.
Interrelationships between sex and dietary lysine on growth performance and carcass composition of finishing boars and gilts1
Authors: Aymerich Pau , Soldevila Carme , Bonet Jordi , Farré Mercè , Gasa Josep , Coma Jaume , Solà-Oriol David
Published: July 2020

The main goals of this study were to determine whether boars and gilts respond differently to the standardized ileal digestible lysine to net energy ratio (SID Lys:NE) and model the response to optimize growth performance. A total of 780 finishing pigs, 390 boars and 390 gilts [Pietrain NN × (Landrace × Large White)], with an initial individual body weight of 70.4 ± 9.2 for boars and 68.7 ± 8.0 kg for gilts, were used in a 41-d dose–response experiment. Pens (13 pigs per pen) were randomly allocated to a dietary treatment (2.64, 3.05, 3.46, 3.86, 4.27 g SID Lys/Mcal NE) by block and sex, with six replicates per treatment and sex. Two isoenergetic diets (2,460 kcal NE/kg), representing the extreme SID Lys:NE, were formulated and then mixed. Pigs were individually weighed at days 0, 22, and 41, when the experiment finished. The differential effect of SID Lys:NE on growth performance and carcass composition between sexes was analyzed with orthogonal polynomial contrasts to compare the linear and quadratic trends in each sex. In addition, broken-line linear (BLL) models to optimize average daily gain (ADG), including average daily feed intake (ADFI) as a covariate, were fitted when possible. As expected, boars had a greater ADG and feed efficiency (G:F; P < 0.001) than gilts, but there was no evidence of differences in ADFI (P = 0.470). Increasing SID Lys:NE had a greater linear impact on boars ADG (P = 0.087), G:F (P = 0.003), and carcass leanness (P = 0.032). In contrast, gilts showed a greater linear increase in SID Lys intake per kg gain (P < 0.001) and feed cost per kg gain (P = 0.005). The best fitting BLL models showed that boars maximized ADG at 3.63 g SID Lys/Mcal NE [95% confidence interval (CI): (3.32 to 3.94)], although another model with a similar fit, compared with the Bayesian information criterion, reported the optimum at 4.01 g SID Lys/Mcal NE [95% CI: (3.60, 4.42)]. The optimum to maximize ADG for gilts was estimated at 3.10 g SID Lys/Mcal NE [95% CI: (2.74, 3.47)]. Thus, the present study confirmed that boars and gilts have a different linear response to SID Lys:NE, explained by the greater protein deposition potential of boars. Likewise, BLL models indicated that boars require a higher SID Lys:NE to maximize ADG from 70 to 89 kg. These results suggest that split feeding of finishing boars and gilts could be beneficial in terms of both performance and cost return.
Stocking rate impacts performance and economics of grazing beef steers on mixed-grass prairies of the Southern Great Plains1
Authors: Beck Paul A , Beck Matthew R , Gunter Stacey A , Biermacher Jon T , Gillen Robert L
Published: July 2020

Stocking rate is a fundamental management factor that has major impacts on animal performance, profitability, and long-term sustainability of native range ecosystems. This research was conducted to determine the effects of stocking rate on performance and economics of growing steers grazing a mixed-grass prairie on a rolling upland red shale ecological site at the Marvin Klemme Range Research Station (35° 25′ N 99° 3′ W). The recommended sustainable stocking rate at this location is suggested to be 25 animal unit days (AUD)/ha. Steers [n = 836, initial body weight (BW) ± SD = 216 ± 11.7 kg] grazed at seven stocking rates ranging from 4.13 ha/steer to 1.83 ha/steer over a 7-yr period, from 1990 to 1996, with year considered the random replication. During the experimental period, overall climatic conditions were favorable for forage production with average growing season precipitation of 118% of the long-term average over the 7-yr experiment, and only 1 yr (1994 with only 57% of the long-term average) with growing season precipitation substantially less than the long-term average. Over the entire summer grazing season, average daily gain (ADG) decreased linearly (P < 0.01) with increasing stocking rate, such that for each additional hectare available per steer ADG increased by 0.05 kg/d (R2 = 0.88). Contrary to ADG, BW gain per hectare over the grazing season increased linearly (P < 0.01) with increasing stocking rate, as stocking rate increased from 4.13 ha/steer to 1.83 ha/steer BW gain per hectare doubled from 33.1 kg/ha to 66.8 kg/ha, respectively. With land costs included in the economic analysis, net return per hectare increased linearly (P < 0.01) from $13 [U.S. Dollars [USD]) at the 4.13 ha/steer to $52/ha at the 1.83 ha/steer. For each additional hectare per steer, net return was reduced by $15.80 (USD)/steer and $15.70 (USD)/ha. In favorable climatic conditions, such as during this 7-yr experiment, economically optimal stocking rates can be more than doubled compared with the stocking rate recommended by the United States Department of Agriculture (USDA) Soil Conservation Service. Increasing stocking rates decrease individual animal performance but maximize BW gain per hectare, which leads to the increasing economic returns observed. Research is needed to determine the long-term implications of these stocking rates during unfavorable growing conditions and setting stocking rates based on seasonal weather patterns and extended weather outlook predictions.
Effects of supplementing docosahexaenoic acid-rich microalgae and antioxidants on beef longissimus lumborum steak color stability and sensory characteristics1,2
Authors: Phelps Kelsey J , Drouillard James S , O’Quinn Travis G , Houser Terry A , Gonzalez John M
Published: July 2020

The objective of this study was to determine the effects of four microalgae and antioxidant feeding regimens on beef longissimus lumborum color stability and palatability. Steers were blocked by weight and randomly assigned to one of four dietary treatments fed during a 45-d feeding period. Treatments (n = 10 per treatment) consisted of a control diet (CON) and control diet plus 100 g∙steer−1∙d−1 microalgae (ALGAE), ALGAE plus antioxidants (103 IU/d vitamin E and Sel-Plex) fed throughout feeding (AOX), and AOX fed for the final 10 d of finishing (LATE). The longissimus lumborum muscle was removed, aged for 14 d, and fabricated into steaks for objective and subjective color and palatability analyses. There were treatment × day of display interactions for a* value and steak surface metmyoglobin percentage (P < 0.01). There were no treatment differences through day 4 of display for a* value (P > 0.16) and day 5 of display for surface metmyoglobin (P > 0.10). By day 10 of display, ALGAE steaks had a smaller a* value than all other treatments (P < 0.01). Steaks from AOX steers had a greater (P < 0.01) a* value than CON steaks, whereas both a* values did not differ from LATE steaks (P > 0.19). By the end of display, ALGAE steaks had more metmyoglobin than the other treatments (P < 0.01). Steaks from AOX steers had less metmyoglobin than CON and LATE steaks (P < 0.04), which did not differ (P > 0.25). Treatment did not affect trained panel ratings (P > 0.15); however, treatment did affect (P < 0.01) off-flavor intensity. Steaks from ALGAE and AOX steers had greater off-flavor ratings than CON steaks (P < 0.03), but did not differ (P = 0.10). Steaks from LATE steers did not differ in off-flavor ratings from the other treatments (P > 0.07). Use of antioxidants improved color stability of steaks from microalgae fed steers; however, panelists still detected off-flavors.
Integrated behavioral health treatments: innovations to achieve population impact
Authors: Bowen Deborah J , Bao Yuhua , Sirey Jo Anne , Ratzliff Anna D H
Published: July 2020

Translational Behavioral Medicine is a journal that brings together relevant scholars and practitioners to produce articles of scientific and practical significance in a variety of fields. Here, we published a call for manuscripts detailing the study of innovations in the field of implementation of integrated care in the USA. We present 13 articles, all peer reviewed and all targeting some aspect of integrated care implementation. These articles include medical and community-based settings, as well as interventions that effectively engage peers, family members, and other social systems to support and extend care. The behavioral health conditions of interest include but were not limited to those that carry the greatest population disease burdens: depression, posttraumatic stress disorder, bipolar disorder, anxiety disorders, and substance abuse disorders. Examples of cross-cutting issues of high interest include research focused on provider and system barriers to integrated care implementation, interventions to improve the use of innovative treatments, disparities in access to care and quality of treatment, the intersection of behavioral health disorders and complex chronic conditions as it affects regimen adherence, health services organization and quality of care, policy effects, innovative methods using health information and mHealth technologies, and personalized/precision medicine. This introduction briefly summarizes some of the relevant topics and background literature. We close with an eye toward future research activities that will continue to advance the field and offer directions to stimulate new research questions in the area.
Lidocaine promotes autophagy of SH-SY5Y cells through inhibiting PI3K/AKT/mTOR pathway by upregulating miR-145
Authors: Wang Zhong , Liu Qin , Lu Jun , Cao Jian , Wang Xiao-Yan , Chen Yong
Published: July 2020

Lidocaine is one of the most common local anesthetics (LA) used in clinical practice and it is neurotoxic. Recent studies suggested that LA, including lidocaine, could exert protective effect over neurotoxicity by promoting autophagy. However, the underlying mechanism was not sufficiently elucidated. This study aimed to explore the mechanism behind. Human neuroblastoma cell line SH-SY5Y was used throughout the whole study. The effect of lidocaine on viability, toxicity of SH-SY5Y cells were analyzed by MTT and lactate dehydrogenase (LDH) assays, respectively. The relative expression of miR-145 was assessed by quantitative reverse transcription-polymerase chain reaction. The impact which lidocaine brought on PI3K/AKT/mTOR pathway and autophagy-related proteins were examined by the western blot assay. LC3B was assessed by immunofluorescence staining. The interaction between miR-145 and AKT3 was conducted by the dual-luciferase reporting assay. Lidocaine inhibited viability of SH-SY5Y cells in a time and dose dependent manner and enhanced the release of LDH in SH-SY5Y cells. Furthermore, the expression of miR-145 and autophagy were enhanced by lidocaine. Transfection with miR-145 inhibitor inhibited the release of LDH and autophagy. miR-145 targeted AKT3 to inhibit PI3K/AKT/mTOR pathway. Finally, lidocaine inactivated PI3K/AKT/mTOR pathways via upregulation of miR-145, and it subsequently promoted autophagy of SH-SY5Y cells. However, silence of miR-145 could reverse the promotion of the autophagy of SH-SY5Y cells. Our results showed that lidocaine promoted autophagy of nerve cells via regulating miR-145 expression and further inactivation of PI3K/AKT/mTOR signaling pathway.
Molecular Characterization of the Purine Degradation Pathway Genes ALA1 and URE1 of the Maize Anthracnose Fungus Colletotrichum graminicola Identified Urease as a Novel Target for Plant Disease Control
Authors: Benatto Perino Elvio Henrique , Glienke Chirlei , de Oliveira Silva Alan , Deising Holger B.
Published: July 2020

Fungal pathogenicity is governed by environmental factors, with nitrogen playing a key role in triggering pathogenic development. Spores germinating on the plant cuticle are exposed to a nitrogen-free environment, and reprograming of nitrogen metabolism is required for bridging the time needed to gain access to the nitrogen sources of the host. Although degradation of endogenous purine bases efficiently generates ammonium and may allow the fungus to bridge the preinvasion nitrogen gap, the roles of the purine degradation pathway and of the key genes encoding allantoicase and urease are largely unknown in plant pathogenic fungi. To investigate the roles of the allantoicase and urease genes ALA1 and URE1 of the maize anthracnose fungus Colletotrichum graminicola in pathogenic development, we generated ALA1:eGFP and URE1:eGFP fusion strains as well as allantoicase- and urease-deficient mutants. Virulence assays, live cell, and differential interference contrast imaging, chemical complementation and employment of a urease inhibitor showed that the purine degradation genes ALA1 and URE1 are required for bridging nitrogen deficiency at early phases of the infection process and for full virulence. Application of the urease inhibitor acetohydroxamic acid did not only protect maize from C. graminicola infection, but also interfered with the infection process of the wheat powdery mildew fungus Blumeria graminis f. sp. tritici, the maize and broad bean rusts Puccinia sorghi and Uromyces viciae-fabae, and the potato late blight pathogen Phytophthora infestans. Our data strongly suggest that inhibition of the purine degradation pathway might represent a novel approach to control plant pathogenic fungi and oomycetes.
Nurses
Authors: Lake Donna
Published: July 2020

Nurses are experienced leaders, understand how to improve quality and access to care, and have the essential skills to be an elected official. Knowing the key tasks to running a campaign is critical to achieving an elected office role. This article presents a call to action for nurse leaders, advice on running for office, and lessons learned from a nurse running for State Senate.
Do Magnet®-Designated Hospitals Perform Better on Medicare's Value-Based Purchasing Program?
Authors: Spaulding Aaron , Hamadi Hanadi , Moody LaRee , Lentz Luanne , Liu Xinliang (Albert) , Wu Yu (Janet)
Published: July 2020

OBJECTIVE

The aim of this study was to explore the relationship between a hospital's Magnet® recognition status, tenure, and its performance in the Hospital Value-Based Purchasing (HVBP) program.

BACKGROUND

Previous studies have sought to determine associations between quality of care provided in inpatient setting and the Magnet Recognition Program®; however, no study has done so using the most recent (FY2017) iteration of the HVBP program, nor determined the influence a hospital's Magnet designation tenure has on HVBP scores.

METHOD

This study used a cross-sectional study design of 2686 hospitals using propensity score matching to reduce bias and improve comparability.

RESULTS

Magnet-designated hospitals were associated with higher total performance, process of care and patient experience of care scores, and lower efficiency score. No association was identified between the length of time hospitals have been Magnet designated.

CONCLUSION

Findings suggest non-Magnet status hospitals need to consider implementing the principles of Magnet into their culture or participation in the Magnet Recognition Program to provide higher quality of care.
African American Nurses' Perspectives on a Leadership Development Program
Authors: Banister Gaurdia E. , Harris Allyssa L. , Townsend Carmela , Raymond Nadia , Masson Patricia R. , Dzurec Laura
Published: July 2020

United States demographics are changing, but that change is yet to be recognized in the makeup of the nursing workforce. The underrepresentation of minorities in nursing is a longstanding problem, resulting in missed opportunities for culturally sensitive care that can foster optimal patient care outcomes. This report describes qualitative analysis of leadership opportunities emerging from a collaborative leadership development program between an urban baccalaureate nursing program and a large healthcare system in the northeast United States. The investigative team used qualitative methods to analyze the experiences of 19 participating African American (AA) RN graduates. Findings demonstrated that, for program participants, active engagement in personal and professional nursing leadership activities was enhanced. The academic-practice leadership program was an effective tool for enculturating AA nurses into the practice environment and into eventual leadership in nursing and healthcare.
Integration of Advance Care Planning Into Clinical Practice
Authors: Simmons-Fields Lisa , Burgermeister Diane , Svinarich David , Hanson Patricia
Published: July 2020

OBJECTIVE

This quality improvement initiative sought to develop a proactive integrated system approach to advance care planning (ACP) through leadership and colleague engagement.

BACKGROUND

Nurse leaders have the capacity to influence the professional competencies of care teams in ACP. Nurse leaders were educated on the importance of ACP, national quality metrics, resources for staff education, and ways to integrate ACP into workflows based on a population management model.

METHODS

The project design is a prospective, mixed method design.

RESULTS

Nurse leader participants demonstrated a significant increase in knowledge of the importance of ACP and evidence-based models to increase staff engagement and competency.

CONCLUSIONS

Study supports nurse leader interventions, promoted engagement of proactive ACP to honor patient choice, and aligns with the mission and vision of one of the largest national Catholic healthcare organizations of being a trusted partner for life.
Abusive Head Trauma and a Delay in Presentation for Care
Authors: Kennedy Juliana M. , Ma Jihyun , Lyden Elizabeth R. , Haney Suzanne B.
Published: July 2020

Objectives

Abusive head trauma (AHT) is the leading cause of death from trauma in children less than 2 years of age. A delay in presentation for care has been reported as a risk factor for abuse; however, there has been limited research on this topic. We compare children diagnosed with AHT to children diagnosed with accidental head trauma to determine if there is a delay in presentation.

Methods

We retrospectively studied children less than 6 years old who had acute head injury and were admitted to the pediatric intensive care unit at a pediatric hospital from 2013 to 2017. Cases were reviewed to determine the duration from symptom onset to presentation to care and the nature of the head injury (abusive vs accidental).

Results

A total of 59 children met inclusion criteria. Patients who had AHT were significantly more likely to present to care more than 30 minutes after symptom onset (P = 0.0015). Children who had AHT were more likely to be younger (median, 4 vs 31 months; P < 0.0001) and receive Medicaid (P < 0.0001) than those who had accidental head trauma. Patients who had AHT were more likely to have a longer length of stay (median, 11 vs 3 days; P < 0.0001) and were less likely to be discharged home than patients who had accidental head trauma (38% vs 84%; P = 0.0005).

Conclusions

Children who had AHT were more likely to have a delayed presentation for care as compared with children whose head trauma was accidental. A delay in care should prompt clinicians to strongly consider a workup for abusive injury.
Paraglaciecola marina sp. nov., isolated from marine alga (Sargassum natans (L.) Gaillon)
Authors: Wang Yan , Zhang Yao , Liu Tingwei , Zhu Xiang , Ma Jin , Su Xiaoman , Kang Jing , Guo Changjiang
Published: July 2020

A novel Gram-stain-negative, straight or curved rod-shaped, non-spore-forming, strictly aerobic, motile bacterium with a single polar flagellum, designated D3211T, was isolated from marine alga collected at the seashore of Yantai, PR China. The organism grew optimally at 24 °C, pH 7.0 and in the presence of 2.0 % (w/v) NaCl. Strain D3211T contained ubiquinone 8 as the major respiratory quinone and C16 : 1
 ω7c and/or C16 : 1
 ω6c, C16 : 0, iso-C17 : 0 and anteiso-C17 : 1 B and/or iso-C17 : 1 I as the major fatty acids. The predominant polar lipids of strain D3211T were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content of strain D3211T was 39.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to

Paraglaciecola arctica

BSs20135T,

Paraglaciecola aestuariivivens

JDTF-33T,

Paraglaciecola aquimarina

KCTC 32108T,

Paraglaciecola mesophila

DSM 15026T,

Paraglaciecola psychrophila

JCM 13954T and

Paraglaciecola polaris

ARK 150T with 97.6, 97.6, 97.5, 97.4, 97.3 and 97.1 % sequence similarities, respectively. Calculated average nucleotide identity and DNA–DNAhybridization values between strain D3211T and its phylogenetically related

Paraglaciecola

species were in the range 70.2–73.4 % and 19.1–20.4 %, respectively. On the basis of polyphasic analyses, strain D3211T represents a novel species of the genus

Paraglaciecola

, for which the name Paraglaciecola marina sp. nov. is proposed. The type strain is D3211T (=KCTC 72122T=MCCC 1K03603T).
Isoptericola halalbus sp. nov., a halotolerant actinobacterium isolated from saline lake sediment
Authors: Ming Hong , Niu Ming-ming , Cheng Li-jiao , Zhang Yan-min , Yi Bing-fang , Xia Ting-ting , Li Meng , Nie Guo-Xing
Published: July 2020

A novel Gram-stain-positive bacterium, designated CFH 91151T, was isolated from sediment collected from a saline lake in Yuncheng, Shanxi Province, PR China. Cells of strain CFH 91151T were rod-or v-shaped, aerobic, non-motile, non-spore-forming and halotolerant. Results of 16S rRNA gene sequence analysis revealed that strain CFH 91151T was closely related to

Isoptericola variabilis

MX5T and

Isoptericola nanjingensis

H17T (98.7 and 98.4% sequence similarity, respectively). The strain grew at 4–45 °C, pH 5.0–9.0 and with 0–14.0 % (w/v) NaCl. Cells were positive for catalase, nitrate was not used and H2S was not produced. Major cellular fatty acids were anteiso-C15 : 0 (62.76 %), anteiso-C17 : 0 (12.09 %) and iso-C15 : 0 (9.46 %). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified phospholipids and three unidentified glycolipids. The menaquinone was MK-9 (H4). The genome size was 4.10 Mbp with a G+C content of 72.4 mol%. The average amino acid identity (ANI) and in silico DNA–DNA hybridization (DDH) values between CFH 91151T and the other species of the genus

Isoptericola

were found to be low (ANIm <87.19 %, ANIb <84.38 % and DDH <29.30 %). Based on physiological properties, chemotaxonomic characteristics and low ANI and DDH results, strain CFH 91151T is considered to represent a novel species, for which the name Isoptericola halalbus sp. nov. is proposed. The type strain is CFH 91151T (=DSM 105976T=KCTC 49061T).
Inverted covariate effects for mutated 2nd vs 1st wave Covid-19: high temperature spread biased for young
Authors: Seligmann Hervé , Iggui Siham , Rachdi Mustapha , Vuillerme Nicolas , Demongeot Jacques
Published: July 2020

(1)

Background

Here, we characterize COVID-19 2nd waves, following a study presenting negative associations between 1st wave COVID-19 spread parameters and temperature;

(2)

Methods

Visual examinations of daily increase in confirmed COVID-19 cases in 124 countries, determined 1st and 2ndwaves in 28 countries;

(3)

Results

1st wave spread rate increases with country mean elevation, temperature, time since wave onset, and median age. Spread rates decrease above 1000m, indicating high UV decrease spread rate. For 2nd waves, associations are opposite: viruses adapted to high temperature and to infect young populations. Earliest 2nd waves started April 5-7 at mutagenic high elevations (Armenia, Algeria). 2nd waves occurred also at warm-to-cold season transition (Argentina, Chile). Spread decreases in most (77%) countries. Death-to-total case ratios decrease during the 2ndwave, also when comparing with the same period for countries where the 1st wave is ongoing. In countries with late 1st wave onset, spread rates fit better 2nd than 1st wave-temperature patterns; In countries with ageing populations (examples: Japan, Sweden, Ukraine), 2nd waves only adapted to spread at higher temperatures, not to infect children.

(4)

Conclusions

1st wave viruses evolved towards lower spread and mortality. 2nd wave mutant COVID-19 strain(s) adapted to higher temperature, infecting children and replace (also in cold conditions) 1st wave COVID-19 strains. Counterintuitively, low spread strains replace high spread strains, rendering prognostics and extrapolations uncertain.
Initial experience in Mexico with convalescent plasma in COVID-19 patients with severe respiratory failure, a retrospective case series
Authors: Martinez-Resendez Michel F. , Castilleja-Leal Fernando , Torres-Quintanilla Alejandro , Rojas-Martinez Augusto , Garcia-Rivas Gerardo , Ortiz-Lopez Rocio , Trevino Victor , Lara-Medrano Reynaldo , Villanueva-Lozano Hiram , Ramirez-Elizondo Teresa , Sanchez-Nava Victor M. , Moreno-Hoyos Francisco , Martinez-Thomae Alfonso , Hernández-Torre Martin , Diaz-Olachea Carlos , Cardona-Huerta Servando , de la Rosa-Pacheco Sylvia , Diaz-Garza Carlos , Reynoso-Lobo Paola , Marroquin-Escamilla Alma R. , Herrera-Gamboa Jessica G. , Alvarado-Monroy Fatima M. , Aguayo-Millan Claudia D. , Villegas-Macedo Francisco F. , Flores-Osorio Jesus Efrain , Davila-Gonzalez Daniel , Diaz-Sanchez María Eugenia , Torre-Amione Guillermo
Published: July 2020

Introduction

Hospital mortality due to COVID-19 in Mexico is high (32%) and as of today, effective treatment options are limited. More effective treatments that shorten hospital stay and reduce mortality are needed. Initial reports for the use of convalescent plasma (CP) therapy for COVID-19 appear promising. We describe a case series of eight patients with impending respiratory failure, who underwent CP therapy.

Methods

Six male and two female (ages 31 to 79) patients that were admitted to the intensive-care unit for severe COVID-19 were transfused with two doses of CP (250 mL per dose, anti-SARS-CoV-2 IgG titers > 1:100). Donors were six SARS-CoV-2 infected males who remained asymptomatic for > 7 days and were negative for two nasopharyngeal RT-PCR tests. Clinical characteristics, inflammatory and cellular injury markers, chest X-ray findings and viral loads were analyzed before and after CP administration. Viral load association to disease severity was further analyzed on a separate cohort of asymptomatic vs hospitalized patients with COVID-19.

Results

Eight patients with respiratory failure were successfully discharged with a median length of stay of 22.5 (IQR 18.25-29.00). After CP therapy, we observed a reduction of C-reactive protein (CRP) (median, 22.80 mg/dL vs. 1.63 mg/dL), and of procalcitonin (median, 0.27 ng/mL vs. 0.13 ng/mL). High-Sensitivity Cardiac Troponin I (hs-cTnI), Brain Natriuretic Peptide (BNP) and Lactate Dehydrogenase (LDH) were lower, and a mild reduction of pulmonary infiltrates by chest X-ray was observed. Lastly, a reduction of viral load was after CP therapy was found. (log, median [IQR], 1.2 [0.70-2.20] vs. 0.25 [0.00-1.78]). We observed no adverse effects.

Conclusions

CP could potentially be an effective therapeutic option for patients with severe COVID-19. Clinical benefit needs to be studied further through randomized controlled trials.
Integrating Remote Monitoring into Heart Failure Patients’ Care Regimen: A Pilot Study
Authors: Sohn Albert , Speier William , Lan Esther , Aoki Kymberly , Fonarow Gregg C , Ong Michael K , Arnold Corey W
Published: July 2020

Background

Heart failure is a debilitating disease affecting millions of adults in the United States, often leading to hospitalizations and hospital readmissions. Around 50% of readmissions due to heart failure are preventable, with lack of adherence to prescribed self-care as a driving factor. Remote tracking and reminders issued by mobile health devices such as activity trackers and smartphone apps could help to promote self-care, which could potentially reduce these readmissions.

Objective

This pilot study used minimally-invasive monitoring technologies and patient-reported outcomes to examine the pragmatic feasibility of a remote monitoring regimen.

Methods

Twenty heart failure patients participated in piloting a remote monitoring regimen. Data collection included: (1) continuous remote monitoring using wrist-worn consumer activity trackers; (2) body weight recording using bathroom scales; (3) dose tracking using smart pill bottles; and (4) patient-reported outcome measures.

Results

Participants were aged 65.3 years on average, 50.0% of the participants were women, and 81.8% of the participants were determined to be New York Heart Association Class III or higher. Over the course of the study, 60.0% of the subjects wore the activity tracker for at least 70% of the hours, and 45.0% used the bathroom scale for more than 70% of the days. For the smart pill bottle, 55.0% of the subjects used it less than 10% of the days. Usage of the activity tracker correlated significantly with changes in Self-Care of Heart Failure Index confidence subscale scores and changes in Seattle Angina Questionnaire scores (P< .05), and usage of the bathroom scale correlated significantly with changes in Seattle Angina Questionnaire scores (P= .04).

Conclusions

The majority of the participants maintained a high adherence to wearing the activity tracker, but had low adherence to using the smart pill bottle. Usage of the bathroom scale was fair, but it received positive reviews from most subjects. Given the observed usage and feedback, we suggest that mobile health-driven interventions consider including an activity tracker and bathroom scale. Furthermore, the data’s correlations with changes in patient-reported outcomes indicate the potential for these devices to be an effective way to remotely monitor heart failure patients.
Are countries’ precautionary actions against COVID-19 effective? An assessment study of 175 countries worldwide
Authors: Alshammari Thamir M. , Alenzi Khalidah A. , Alnofal Fatemah A , Fradees Ghada , Altebainawi Ali F.
Published: July 2020

Background

The Coronavirus Disease 2019 (COVID-19) pandemic has affected many countries negatively, particularly in terms of their health care and financial systems. Numerous countries have attempted to employ precautions to address this pandemic. This study was aimed at exploring and assessing the precautionary actions taken by 175 countries on six continents to prevent the spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2).

Methods

An observational study was conducted based on data collected during the period from December 31, 2019, until the end of April 2020. Several data were extracted, including information related to the date of the first reported case of SARS-CoV-2, total confirmed cases, total active cases, and more. In addition, seven validated indicators were used to assess the countries’ preparedness and precautionary actions.

Findings

A total of 175 countries were included in the study. The total COVID-19 infection rate increased exponentially and rapidly in North America and Europe from March to April. The application of the precautions (indicators) varied between countries. School closures, quarantines and curfews were the most applied indicators among all countries. As for the relationship between the indicators and their effects on the infection rate, Italy and Spain were the top countries in Europe and adopted all indicators. Nevertheless, they faced high infection rates: 239,639 and 205,463 COVID-19 cases in Spain, and Italy, respectively.

Interpretation

The precautionary actions might have played a role in limiting the spread of COVID-19 in several countries. However, many countries did not benefit from applying these indicators.

Funding

No funding sources have been used for this work