bluuebunny/crossref_metadata_2025 huggingface dataset:
From Foxtail Millet Husk (Waste) to Bioactive Phenolic Extracts Using Deep Eutectic Solvent Extraction and Evaluation of Antioxidant, Acetylcholinesterase, and α-Glucosidase Inhibitory Activities
Authors: Wang Chunqing , Li Zhenzhen , Xiang Jinle , Johnson Joel , Zheng Bailiang , Luo Lei , Beta Trust
Published: March 2023

Foxtail millet husk (FMH) is generally removed and discarded during the first step of millet processing. This study aimed to optimize a method using deep eutectic solvents (DESs) combined with ultrasonic-assisted extraction (UAE) to extract phenols from FMH and to identify the phenolic compositions and evaluate the biological activities. The optimized DES comprised L-lactic acid and glycol with a 1:2 molar ratio by taking the total flavonoid content (TFC) and total phenolic content (TPC) as targets. The extraction parameters were optimized to maximize TFC and TPC, using the following settings: liquid-to-solid ratio of 25 mL/g, DES with water content of 15%, extraction time of 41 min and temperature of 51 °C, and ultrasonic power at 304 W. The optimized UAE-DES, which produced significantly higher TPC, TFC, antioxidant activity, α-glucosidase, and acetylcholinesterase inhibitory activities compared to conventional solvent extraction. Through UPLC–MS, 12 phenolic compounds were identified, with 1-O-p-coumaroylglycerol, apigenin-C-pentosyl-C-hexoside, and 1-O-feruloyl-3-O-p-coumaroylglycerol being the main phenolic components. 1-O-feruloyl-3-O-p-coumaroylglycerol and 3,7-dimethylquercetin were identified first in foxtail millet. Our results indicated that FMH could be exploited by UAE-DES extraction as a useful source of naturally derived antioxidants, along with acetylcholinesterase and α-glucosidase inhibitory activities.
Magnolol Loaded on Carboxymethyl Chitosan Particles Improved the Antimicrobial Resistance and Storability of Kiwifruits
Authors: Mo Feixu , Li Wenzhi , Long Youhua , Li Rongyu , Ding Yi , Li Ming
Published: March 2023

Magnolol is a natural compound extracted from the traditional Chinese medicine Magnolia officinalis, which exhibits antimicrobial properties. However, magnolol is insoluble in water and consists of a phenolic hydroxyl group, which is volatile; these factors hinder its application. In this study, a safe and environmentally friendly method to improve the microbial resistance and storability of harvested fruits is developed using the water-soluble carrier carboxymethyl chitosan (CMCS) and magnolol. Magnolol was loaded on CMCS particles to form Magnolol@CMCS antimicrobial particles, a preservation coating agent. Magnolol@CMCS particles effectively solved the problems of water insolubility and agglomeration of magnolol and reduced the size distribution D50 value of magnolol from 0.749 to 0.213 μm. Magnolol@CMCS particles showed greater toxicity against Staphylococcus aureus, Escherichia coli, and Botryosphaeria dothidea than that of magnolol alone, with effective medium concentration (EC50) values of 0.9408, 142.4144, and 8.8028 μg/mL, respectively. Kiwifruit treated with the Magnolol@CMCS solution showed delayed changes in fruit hardness and soluble solid and dry matter contents and significantly higher ascorbic acid (vitamin C) and soluble total sugar contents and sugar:acid ratios compared with that of the control fruit. In addition, no disease spots were observed on fruit treated with the Magnolol@CMCS solution within 7 days after inoculation with B. dothidea. In conclusion, Magnolol@CMCS particles showed antimicrobial activity on harvested fruits, effectively delayed the hardness and nutritional changes of fruits during storage, and improved the storability of kiwifruit.
Sequential Predictors for Uncertain Euler–Lagrange Systems with Large Transmission Delays
Authors: Shen Shaobo , Fu Liyue , Song Aiguo , Li Tao
Published: March 2023

This paper investigates the state prediction problems for uncertain Euler–Lagrange systems with large time delays during data transmissions. A set of sequential predictors is proposed to estimate the actual real-time states of the systems by using the delayed information of measurements. The arbitrarily large delays are handled by applying adequate numbers of serial sub-predictors. Meanwhile, the novel prediction structure of each subsystem is designed to deal with nonlinearities and unknown dynamics in the systems. Then, the predictor design is extended to the case without using delayed velocity measurements by updating the structure of the first sub-predictor. Sufficient conditions for the design of predictor gains, ensuring the boundness of prediction errors, are obtained through Lyapunov–Krasovskii functionals. The effectiveness and robustness of the uncertainties of the proposed method are verified by comparative results in simulations.
Fractional Order Runge–Kutta Methods
Authors: Ghoreishi Farideh , Ghaffari Rezvan , Saad Nasser
Published: March 2023

This paper presents a new class of fractional order Runge–Kutta (FORK) methods for numerically approximating the solution of fractional differential equations (FDEs). We construct explicit and implicit FORK methods for FDEs by using the Caputo generalized Taylor series formula. Due to the dependence of fractional derivatives on a fixed base point, in the proposed method, we had to modify the right-hand side of the given equation in all steps of the FORK methods. Some coefficients for explicit and implicit FORK schemes are presented. The convergence analysis of the proposed method is also discussed. Numerical experiments are presented to clarify the effectiveness and robustness of the method.
Measuring Patient Experience and Patient Satisfaction—How Are We Doing It and Why Does It Matter? A Comparison of European and U.S. American Approaches
Authors: Friedel Anna Lena , Siegel Sonja , Kirstein Cedric Fabian , Gerigk Monja , Bingel Ulrike , Diehl Anke , Steidle Oliver , Haupeltshofer Steffen , Andermahr Bernhard , Chmielewski Witold , Kreitschmann-Andermahr Ilonka
Published: March 2023

(1) Background: Patients’ experiences and satisfaction with their treatment are becoming increasingly important in the context of quality assurance, but the measurement of these parameters is accompanied by several disadvantages such as poor cross-country comparability and methodological problems. The aim of this review is to describe and summarize the process of measuring, publishing, and utilizing patient experience and satisfaction data in countries with highly developed healthcare systems in Europe (Germany, Sweden, Finland, Norway, the United Kingdom) and the USA to identify possible approaches for improvement. (2) Methods: Articles published between 2000 and 2021 that address the topics described were identified. Furthermore, patient feedback in social media and the influence of sociodemographic and hospital characteristics on patient satisfaction and experience were evaluated. (3) Results: The literature reveals that all countries perform well in collecting patient satisfaction and experience data and making them publicly available. However, due to the use of various different questionnaires, comparability of the results is difficult, and consequences drawn from these data remain largely unclear. (4) Conclusions: Surveying patient experience and satisfaction with more unified as well as regularly updated questionnaires would be helpful to eliminate some of the described problems. Additionally, social media platforms must be considered as an increasingly important source to expand the range of patient feedback.
The Mediating Role of Work Satisfaction in the Relationship between Organizational Communication Satisfaction and Organizational Commitment of Healthcare Professionals: A Cross-Sectional Study
Authors: Dalal Helmy Jameel Abu , Ramoo Vimala , Chong Mei Chan , Danaee Mahmoud , Aljeesh Yousef I. , Rajeswaran Veshaaliini Uma
Published: March 2023

The factors that influence the organizational commitment of healthcare professionals, particularly organizational communication and work satisfaction, are essential for all healthcare organizations. This is particularly important for those who are under the pressure of high demand, economic constraints, and staff shortages. This study examined the relationship between organizational communication satisfaction and organizational commitment among healthcare professionals and the mediating role of work satisfaction in the relationship. A validated self-administered questionnaire and a universal sampling approach were used to conduct a cross-sectional survey of 235 healthcare professionals in the Gaza Strip, Palestine. The data were analyzed using SPSS version 25 and SmartPLS software to perform partial least squares structural equation modeling (PLS-SEM). A STROBE checklist was used to report the results. The results show a significant positive correlation between all measured variables. Work satisfaction partially mediates the relationship between organizational communication satisfaction and organizational commitment. The results of the PLS-SEM analyses suggest that communication satisfaction and work satisfaction account for 61% of the variation in organizational commitment. This study reveals that work satisfaction and communication satisfaction are imperative to building a sense of commitment in healthcare professionals. These results reinforce the existing evidence on the factors that influence the organizational commitment of healthcare professionals. Strategies to better shape internal communication practices and improve the work environment through regular feedback to healthcare professionals are essential to strengthening their organizational commitment.
Exploring Knowledge about Fang Traditional Medicine: An Informal Health Seeking Behaviour for Medical or Cultural Afflictions in Equatorial Guinea
Authors: Jimenez-Fernandez Raquel , Rodriguez Vázquez Rocío , Marín-Morales Dolores , Herraiz-Soria Elena , Losa-Iglesias Marta Elena , Becerro-de-Bengoa-Vallejo Ricardo , Corral-Liria Inmaculada
Published: March 2023

This study explores a range of informal health-seeking behaviors, including the use of Fang Traditional Medicine (FTM) for medical or cultural afflictions in Equatorial Guinea (EQ), the therapeutic methods used, the health problems handled, the learning process, traditional medicine user profiles and the social images of Fang Traditional Healers (FTHs). Ethnography was employed as a qualitative strategy using emic–etic approaches. Semi-structured interviews were conducted with 45 individuals, including 6 community leaders, 19 tribal elders, 7 healthcare professionals, 11 FTHs and 2 relatives of traditional healers in 5 districts of EQ. FTM offers a cure for malaria and treatments for reproductive health issues, bone fractures and cultural illnesses. Several methods used to learn FTM are based on empirical observation, and without the need for traditional schooling, unlike with Western medical professionals: for example, watching a family member, or the spirits or ancestors, can reveal healing knowledge. Materials from forests, including tree barks and plants, and rituals are used to keep Fang populations healthy; in addition, two rituals known as “osuiñ” and “etoak” (infusions of tree barks with the blood of sacrificed animals) are the most commonly used treatments. In addition, elders and women are the most active consumers of FTM. FTM plays a relevant role in curing medical and cultural afflictions in Fang communities. The informal health-seeking behavior among the Fang community is conditioned by the explanation model of illness.
The Parental Stress Scale: Psychometric Properties in Pediatric Hospital Emergency Setting
Authors: Montoro-Pérez Néstor , Escribano Silvia , Richart-Martínez Miguel , Mármol-López María Isabel , Montejano-Lozoya Raimunda
Published: March 2023

Parental psychological distress has been identified as a predisposing factor in attendance at and the inappropriate use of hospital pediatric emergency departments (PEDs). The aim of the study was to validate the Parental Stress Scale (PSS), a 12-item Spanish scale, in parents seeking care at PEDs. The study involved 270 participants with a mean age of 37.9 (SD = 6.76) years, of which 77.4% were women. The properties of the PSS were analyzed. The scale showed adequate internal consistency for the different factors (0.80 for the “Stressors” factor and 0.78 for the “Baby’s Rewards” factor) and optimal model fit (chi-square = 107.686; df = 53; CFI = 0.99; TLI = 0.98; RMSEA = 0.028; 90% CI = 0.00–0.05). The 12-item Spanish version of the PSS is a valid and reliable instrument for assessing the stress levels of parents seeking care in PEDs.
The Effect of Environmental Factors on Immunological Pathways of Asthma in Children of the Polish Mother and Child Cohort Study
Authors: Sara Żywiołowska-Smuga , Joanna Jerzyńska , Daniela Podlecka , Kinga Polańska , Agnieszka Brzozowska
Published: March 2023

The FOXP3 transcription factor is a marker of regulatory T cells (Tregs), and is essential in the process of their activation and proper expression by promoting immune homeostasis. To assess the influence of the environment on the development of asthma, we hypothesized that in our cohort, exposure to environmental factors is associated with asthma risk in children, and that FOXP3 levels vary with their incidence and are negatively correlated with developing asthma. This prospective study conducted in Poland uses a cohort of 85 children (42 with and 43 without asthma diagnosis) aged 9 to 12 years recruited for the Polish Mother and Child Cohort Study. We collected questionnaires and organized visits to assess patients’ clinical condition (skin prick tests, lung function assessments). Blood samples were taken to determine immune parameters. Breastfed children had lower risk of asthma. Asthma risk was higher in children who live in the city, with antibiotic course before the age of 2 and antibiotic therapy more than twice a year. Environmental factors were associated with childhood asthma. Breastfeeding, the coexistence of other allergic diseases, and the frequency of housekeeping affect FOXP3 levels, which are negatively correlated with the risk of asthma.
Potential Carcinogens in Makeup Cosmetics
Authors: Balwierz Radosław , Biernat Paweł , Jasińska-Balwierz Agata , Siodłak Dawid , Kusakiewicz-Dawid Anna , Kurek-Górecka Anna , Olczyk Paweł , Ochędzan-Siodłak Wioletta
Published: March 2023

Facial makeup cosmetics are commonly used products that are applied to the skin, and their ingredients come into contact with it for many years. Consequently, they should only contain substances that are considered safe or used within an allowable range of established concentrations. According to current European laws, all cosmetics approved for use should be entirely safe for their users, and the responsibility for this lies with manufacturers, distributors, and importers. However, the use of cosmetics can be associated with undesirable effects due to the presence of certain chemical substances. An analysis of 50 random facial makeup cosmetics commercially available on the European Union market and manufactured in six European countries was carried out, concerning the presence of substances with potential carcinogenic properties, as described in recent years in the literature. Nine types of facial makeup cosmetics were selected, and their compositions, as declared on the labels, were analyzed. The carcinogens were identified with information present in the European CosIng database and according to the Insecticide Resistance Action Committee’s (IRAC) classification. As a result, the following potential carcinogens were identified: parabens (methylparaben, propylparaben, butylparaben, and ethylparaben), ethoxylated compounds (laureth-4, lautreth-7, or ethylene glycol polymers known as PEG), formaldehyde donors (imidazolidinyl urea, quaternium 15, and DMDM hydantoin), and ethanolamine and their derivatives (triethanolamine and diazolidinyl urea), as well as carbon and silica. In conclusion, all of the analyzed face makeup cosmetics contain potential carcinogenic substances. The literature review confirmed the suppositions regarding the potential carcinogenic effects of selected cosmetic ingredients. Therefore, it seems necessary to carry out studies on the long-term exposure of compounds present in cosmetics and perhaps introduce stricter standards and laws regulating the potential presence of carcinogens and their activity in cosmetics.
Effects of High Temperature Exposure on the Wingate Test Performance in Male University Students
Authors: Toro-Román Víctor , Prieto-González Isaac , Siquier-Coll Jesús , Bartolomé Ignacio , Grijota Francisco , Maynar-Mariño Marcos
Published: March 2023

It has been suggested that heat exposure prior to exercise could induce changes in anaerobic exercise. Therefore, the purpose of this study was to observe the effects of high temperature heat exposure prior to an anaerobic test. Twenty-one men (age: 19.76 ± 1.22 years; height: 1.69 ± 0.12 m; weight: 67.89 ± 11.78 kg) voluntarily participated in this investigation. All of them performed two Wingate tests, vertical jump and macronutrient intake control. On the first day, the test was performed under normal environmental conditions. On the second day, it was performed in a similar way, but with previous exposure to heat at high sauna temperatures (15 min; 100 ± 2 °C). There were no differences in the vertical jump and macronutrient intake. However, the results showed an improvement in power (W) (p < 0.05), relative power (W/kg) (p < 0.01) and revolutions per minute (p < 0.05) 10 s after the start of the test. There was also an increase in thigh (p < 0.01) and skin temperature (p < 0.01) with pre-heat exposure. The results obtained suggest that this pre-exercise protocol could improve power in short and intensive actions.
Spatio-Temporal Difference in Agricultural Eco-Efficiency and Its Influencing Factors Based on the SBM-Tobit Models in the Yangtze River Delta, China
Authors: Shi Lin , Shi Xiaofei , Yang Fan , Zhang Lixue
Published: March 2023

In the Yangtze River Delta region, where the agricultural economy is well developed and agricultural pollution and carbon emissions are significant, a regional study of AEE (Agricultural Eco-Efficiency) is crucial to reducing agricultural environmental pollution, improving the rationalization of agricultural production layout, and promoting the realization of low-carbon goals. The SBM-Tobit model and GIS were employed to analyze AEE based on the carbon emission evaluation system in the spatial and temporal characteristics, as well as the influencing factors and the migration path of the center of gravity in the “low carbon” context. A rational plan of agricultural production was proposed according to the results. The following results were obtained: (1) The level of AEE in the Yangtze River Delta region was high, and the region exhibited a U-shaped curve change from 2000 to 2020, with a fluctuating decrease from 2000 to 2003 and a fluctuating increase from 2004 to 2020. The regional spatial development balance was enhanced, while there was a spatial incongruity in the development process of AEE enhancement, high in the southwest and low in the northeast; (2) AEE generally had a high regionalized agglomeration of low–low in the southwest and high–high in the northeast. Nonetheless, temporal heterogeneity was observed in spatial correlation, and the correlation weakened with time variation; (3) Urbanization level, agricultural production structure, crop cultivation structure, and fertilizer application intensity were the main factors influencing AEE in the Yangtze River Delta region; (4) The center of gravity of AEE in the Yangtze River Delta region shifted to the southwest under the influence of “low-carbon” related policies. Therefore, the improvement of AEE in the Yangtze River Delta region should focus on inter-regional coordination and linkages, rational planning of production factors, and the formulation of measures under relevant carbon policies.
Bibliometric Analysis of Global Scientific Production on COVID-19 and Vaccines
Authors: Sousa Neto Antonio Rosa de , Carvalho Ana Raquel Batista de , Ferreira da Silva Márcia Daiane , Rêgo Neta Marly Marques , Sena Inara Viviane de Oliveira , Almeida Rosângela Nunes , Filha Francidalma Soares Sousa Carvalho , Lima e Silva Laianny Luize , Costa Girlene Ribeiro da , Lira Ivana Mayra da Silva , Portela Denise Maria Meneses Cury , Oliveira e Silva Amanda Tauana , Rabêlo Cristiane Borges de Moura , Valle Andreia Rodrigues Moura da Costa , Moura Maria Eliete Batista , Freitas Daniela Reis Joaquim de
Published: March 2023

This bibliometric analysis aims to analyze the global scientific production of COVID-19 and vaccines. First, a search for scientific articles was performed using the advanced query in the Web of Science™ database, more precisely in its core collection, on 18 February 2023. Data from 7754 articles were analyzed using the Bibliometrix R package and the Biblioshiny application. The evaluated articles were published mainly in 2022 (60%). The scientific journals that published the most about COVID-19 and vaccines were “Vaccines”, “Vaccine” and “Human Vaccines & Immunotherapeutics”. The University of Oxford was the most productive institution, with the authors of the articles mainly originating from the United States, China and the United Kingdom. The United States, despite having carried out the most significant number of collaborations, published mainly with local researchers. The 15 most cited articles and the KeyWords Plus™ evidenced the focus of the published articles on the safety and efficacy of vaccines against COVID-19, as well as on the evaluation of vaccine acceptance, more specifically on vaccine hesitancy. Research funding came primarily from US government agencies.
Study on the Dynamic Relationship between Chinese Residents’ Individual Characteristics and Commercial Health Insurance Demand
Authors: Tian Ling , Dong Haisong
Published: March 2023

Based on the time series data of age characteristics, household registration characteristics, gender characteristics, education characteristics, marriage characteristics and commercial health insurance density of Chinese residents from 1997 to 2020, this paper aims to explore the dynamic relationship between the individual characteristics of Chinese residents and the demand for commercial health insurance by means of impulse response and variance decomposition analysis using an SVAR model. The results show that the age characteristics, household registration characteristics, gender characteristics, education characteristics and marriage characteristics of Chinese residents have a significant impact on the demand for commercial health insurance, but there is a time lag. There is a long-term equilibrium relationship between them: In terms of age characteristics and gender characteristics, the former has a positive effect in the short term and a significant inhibition on commercial health insurance demand in the long term, while the latter has the opposite. In terms of household registration characteristics, education characteristics and marriage characteristics, there are positive effects on the whole and negative effects in a particular period.
Factors Associated with the Quality-of-Life of Young Unpaid Carers: A Systematic Review of the Evidence from 2003 to 2019
Authors: Bou Camille
Published: March 2023

The aim of this review was to identify factors influencing the quality of life (QoL) of young people providing care for family members with chronic illnesses, disabilities, and/or mental health and substance abuse problems (young unpaid carers; YC), as well as the social-care related QoL measures. Focused and broad search strategies were performed in four databases, identifying 3145 articles. Following screening, lateral searches, and quality appraisal, 54 studies were included for synthesis. An inductive approach was used to synthesise the findings, grouping factors associated with YC QoL into interrelated themes: “perceived normality of role and identifying as a carer”, “social support from formal and unpaid networks”, “caring demands and their impact”, and “coping strategies”. No social-care related QoL measures for YC were found. This systematic review provides groundwork for the development of such a tool and emphasises the need for further studies allowing the investigation of the interrelated factors affecting YC QoL.
Coping of Chronically-Ill Patients during the COVID-19 Pandemic: Comparison between Four Groups
Authors: Łuc Mateusz , Pawłowski Marcin , Jaworski Arkadiusz , Fila-Witecka Karolina , Szcześniak Dorota , Augustyniak-Bartosik Hanna , Zielińska Dorota , Stefaniak Aleksandra , Pokryszko-Dragan Anna , Chojdak-Łukasiewicz Justyna , Krajewska Magdalena , Pawłowski Tomasz , Szepietowski Jacek C. , Rymaszewska Joanna
Published: March 2023

In many countries, the COVID-19 pandemic led to healthcare reorganization limiting access to diagnostic or therapeutic procedures for chronically-ill patients. In this article, we describe the psychological consequences and coping strategies of several groups of chronically-ill patients. During the cross-sectional survey conducted in 2020, we enrolled 398 patients with four different chronic conditions (psoriasis, multiple sclerosis, and patients who have undergone a kidney transplant or received dialysis). The study sample was examined regarding the experienced stress levels (Perceived Stress Scale) and coping strategies (Brief-COPE). All four groups of patients most commonly declared using problem-focused coping strategies and least commonly reported the use of avoidant coping. Higher levels of perceived stress strongly correlated with self-blaming. The participants who declared previous psychiatric treatment or psychotherapy were more likely to use self-blaming, behavioral disengagement, substance use, and avoidant coping, while previous psychotherapy additionally correlated with emotion-focused coping. Group comparison identifies patients with a chronic neurological disease, such as multiple sclerosis, at higher risk of a less beneficial coping profile than kidney transplant recipients. Further focus on education and early interventions in at-risk individuals is needed, and widely targeted mental health programs are indicated in order to improve the mental health of patients suffering from chronic diseases.
Brazilian Immigrant Parents’ Preferences for Content and Intervention Modalities for the Design of a Family-Based Intervention to Promote Their Preschool-Age Children’s Healthful Energy Balance-Related Behaviors
Authors: Vilasboas Thaís , Le Qun , Greaney Mary L. , Lindsay Ana Cristina
Published: March 2023

Brazilians are a rapidly growing ethnic immigrant population in the United States (U.S.), and there is a lack of childhood obesity prevention interventions addressing the needs of Brazilian preschool-age children. Using the family ecological model (FEM) as a guide, this developmental cross-sectional study assessed the preferences (content, intervention modality, and language) of 52 individual Brazilian immigrant parents (27 mothers, 25 fathers) for a family-based intervention to promote healthful energy balance-related behaviors (EBRB). Overall, 85% or more of parents reported being interested or very interested in content related to five of the seven assessed EBRBs (increasing fruits and vegetables, reducing unhealthy foods and sugar-sweetened beverages, increasing physical activity, and reducing screen time). Parent-preferred intervention modalities were group sessions delivered by community health workers (CHWs, 86.5%), email (84.6%), and messaging (78.8%), with most parents (71.2%) indicating a preference for content in Portuguese. Interventions integrating multiple components, such as group sessions offered by CHWs and text messaging using SMS and WhatsApp, should be considered. Future steps for intervention development should include investigating different communication channels and their integration into a culturally and linguistically tailored family-based intervention designed to promote healthful EBRBs of preschool-age children in Brazilian families living in the U.S.
Responses of Soil Collembolans to Land Degradation in a Black Soil Region in China
Authors: Ma Chen , Nie Runze , Du Guoming
Published: March 2023

Land degradation in black soil regions has a significant effect on belowground systems, and Collembolans can adequately indicate environmental changes in the soil. However, there is currently a knowledge gap in the literature regarding the responses of soil Collembolans to land degradation. In order to better understand this issue, in this study, a total of 180 soil Collembolan samples were collected from four habitats with varying degrees of land degradation in the Songnen Plain, namely a no land-degradation habitat (NLD), light land-degradation habitat (LLD), moderate land-degradation habitat (MLD) and severe land-degradation habitat (SLD). The results reveal that the different degrees of land degradation caused some differences in the taxonomic composition of the Collembolans; however, the majority of the Collembolan species are distributed relatively evenly. Proisotoma minima are always a dominant species during the study period. Seasonal variations are observed in the abundance, richness and diversity levels. In the severe land-degradation habitats (SLD), the abundance, richness, diversity and community complexity of the Collembolans are aways at the lowest levels. In addition, Proisotoma minima is negatively correlated with a majority of the species of Collembolans in the low levels of the land-degradation habitats, whereas they are positively correlated with most of the other species in the high levels. Epedaphic and euedaphic Collembolans responded to land degradation more obviously. The structural equation model (SEM) displays that soil Collembolan communities respond negatively to land degradation. Overall, our results provide implications that soil Collembolan communities are affected by land degradation, and that different taxa of soil Collembolans respond to degradation in numerous ways.
Be Quiet! Effects of Competing Speakers and Individual Characteristics on Listening Comprehension for Primary School Students
Authors: Visentin Chiara , Pellegatti Matteo , Garraffa Maria , Di Domenico Alberto , Prodi Nicola
Published: March 2023

Students learn in noisy classrooms, where the main sources of noise are their own voices. In this sound environment, students are not equally at risk from background noise interference during lessons, due to the moderation effect of the individual characteristics on the listening conditions. This study investigates the effect of the number of competing speakers on listening comprehension and whether this is modulated by selective attention skills, working memory, and noise sensitivity. Seventy-one primary school students aged 10 to 13 years completed a sentence comprehension task in three listening conditions: quiet, two competing speakers, and four competing speakers. Outcome measures were accuracy, listening effort (response times and self-reported), motivation, and confidence in completing the task. Individual characteristics were assessed in quiet. Results showed that the number of competing speakers has no direct effects on the task, whilst the individual characteristics were found to moderate the effect of the listening conditions. Selective attention moderated the effects on accuracy and response times, working memory on motivation, and noise sensitivity on both perceived effort and confidence. Students with low cognitive abilities and high noise sensitivity were found to be particularly at risk in the condition with two competing speakers.
Analysis and Visualization of Vessels’ RElative MOtion (REMO)
Authors: Ban Hyowon , Kim Hye-jin
Published: March 2023

This research is a pilot study to develop a maritime traffic control system that supports the decision-making process of control officers, and to evaluate the usability of a prototype tool developed in this study. The study analyzed the movements of multiple vessels through automatic identification system (AIS) data using one of the existing methodologies in GIScience, the RElative MOtion (REMO) approach. The REMO approach in this study measured the relative speed, delta-speed, and the azimuth of each vessel per time unit. The study visualized the results on electronic navigational charts in the prototype tool developed, V-REMO. In addition, the study conducted a user evaluation to assess the user interface (UI) of V-REMO and to future enhance the usability. The general usability of V-REMO, the data visualization, and the readability of information in the UI were tested through in-depth interviews. The results of the user evaluation showed that the users needed changes in the size, position, colors, and transparency of the trajectory symbols in the digital chartmap view of V-REMO for better readability and easier manipulation. The users also indicated a need for multiple color schemes for the spatial data and more landmark information about the study area in the chartmap view.
Does Time Smoothen Space? Implications for Space-Time Representation
Authors: Sang Neil
Published: March 2023

The continuous nature of space and time is a fundamental tenet of many scientific endeavors. That digital representation imposes granularity is well recognized, but whether it is possible to address space completely remains unanswered. This paper argues Hales’ proof of Kepler’s conjecture on the packing of hard spheres suggests the answer to be “no”, providing examples of why this matters in GIS generally and considering implications for spatio-temporal GIS in particular. It seeks to resolve the dichotomy between continuous and granular space by showing how a continuous space may be emergent over a random graph. However, the projection of this latent space into 3D/4D imposes granularity. Perhaps surprisingly, representing space and time as locally conjugate may be key to addressing a “smooth” spatial continuum. This insight leads to the suggestion of Face Centered Cubic Packing as a space-time topology but also raises further questions for spatio-temporal representation.
Young Astrocytic Mitochondria Attenuate the Elevated Level of CCL11 in the Aged Mice, Contributing to Cognitive Function Improvement
Authors: Tashiro Ryosuke , Ozaki Dan , Bautista-Garrido Jesus , Sun Guanghua , Obertas Lidiya , Mobley Alexis S. , Kim Gab Seok , Aronowski Jaroslaw , Jung Joo Eun
Published: March 2023

Aging drives cognitive decline, and mitochondrial dysfunction is a hallmark of age-induced neurodegeneration. Recently, we demonstrated that astrocytes secrete functional mitochondria (Mt), which help adjacent cells to resist damage and promote repair after neurological injuries. However, the relationship between age-dependent changes in astrocytic Mt function and cognitive decline remains poorly understood. Here, we established that aged astrocytes secret less functional Mt compared to young astrocytes. We found the aging factor C-C motif chemokine 11 (CCL11) is elevated in the hippocampus of aged mice, and that its level is reduced upon systemic administration of young Mt, in vivo. Aged mice receiving young Mt, but not aged Mt improved cognitive function and hippocampal integrity. Using a CCL11-induced aging-like model in vitro, we found that astrocytic Mt protect hippocampal neurons and enhance a regenerative environment through upregulating synaptogenesis-related gene expression and anti-oxidants that were suppressed by CCL11. Moreover, the inhibition of CCL11-specific receptor C-C chemokine receptor 3 (CCR3) boosted the expression of synaptogenesis-related genes in the cultured hippocampal neurons and restored the neurite outgrowth. This study suggests that young astrocytic Mt can preserve cognitive function in the CCL11-mediated aging brain by promoting neuronal survival and neuroplasticity in the hippocampus.
FTIR Analysis of Renal Tissue for the Assessment of Hypertensive Organ Damage and proANP31–67 Treatment
Authors: Pioppi Leonardo , Tombolesi Niki , Parvan Reza , da Silva Gustavo Jose Justo , Altara Raffaele , Paolantoni Marco , Morresi Assunta , Sassi Paola , Cataliotti Alessandro
Published: March 2023

The kidneys are one of the main end organs targeted by hypertensive disease. Although the central role of the kidneys in the regulation of high blood pressure has been long recognized, the detailed mechanisms behind the pathophysiology of renal damage in hypertension remain a matter of investigation. Early renal biochemical alterations due to salt-induced hypertension in Dahl/salt-sensitive rats were monitored by Fourier-Transform Infrared (FTIR) micro-imaging. Furthermore, FTIR was used to investigate the effects of proANP31–67, a linear fragment of pro-atrial natriuretic peptide, on the renal tissue of hypertensive rats. Different hypertension-induced alterations were detected in the renal parenchyma and blood vessels by the combination of FTIR imaging and principal component analysis on specific spectral regions. Changes in amino acids and protein contents observed in renal blood vessels were independent of altered lipid, carbohydrate, and glycoprotein contents in the renal parenchyma. FTIR micro-imaging was found to be a reliable tool for monitoring the remarkable heterogeneity of kidney tissue and its hypertension-induced alterations. In addition, FTIR detected a significant reduction in these hypertension-induced alterations in the kidneys of proANP31–67-treated rats, further indicating the high sensitivity of this cutting-edge imaging modality and the beneficial effects of this novel medication on the kidneys.
Ameliorative Effect of Posidonia oceanica on High Glucose-Related Stress in Human Hepatoma HepG2 Cells
Authors: Vasarri Marzia , Barletta Emanuela , Stio Maria , Bergonzi Maria Camilla , Galli Andrea , Degl’Innocenti Donatella
Published: March 2023

Metabolic disorders characterized by elevated blood glucose levels are a recognized risk factor for hepatocellular carcinoma (HCC). Lipid dysregulation is critically involved in the HCC progression, regulating energy storage, metabolism, and cell signaling. There is a clear link between de novo lipogenesis in the liver and activation of the NF-κB pathway, which is involved in cancer metastasis via regulation of metalloproteinases MMP-2/9. As conventional therapies for HCC reach their limits, new effective and safe drugs need to be found for the prevention and/or adjuvant therapy of HCC. The marine plant Posidonia oceanica (L.) Delile is endemic to the Mediterranean and has traditionally been used to treat diabetes and other health disorders. The phenol-rich leaf extract of Posidonia oceanica (POE) is known to have cell-safe bioactivities. Here, high glucose (HG) conditions were used to study lipid accumulation and fatty acid synthase (FASN) expression in human HepG2 hepatoma cells using Oil Red O and Western blot assays. Under HG conditions, the activation status of MAPKs/NF-κB axis and MMP-2/9 activity were determined by Western blot and gelatin zymography assays. The potential ameliorative role of POE against HG-related stress in HepG2 cells was then investigated. POE reduced lipid accumulation and FASN expression with an impact on de novo lipogenesis. Moreover, POE inhibited the MAPKs/NF-κB axis and, consequently, MMP-2/9 activity. Overall, these results suggest that P. oceanica may be a potential weapon in the HCC additional treatment.
Transcriptomic Profile of Canine Mammary Ductal Carcinoma
Authors: Santos Driéle B. , Fernandez Geysson J. , Pardini Luciana M. C. , Pardini Maria Inês M. C. , Ferrasi Adriana C.
Published: March 2023

Dogs can be excellent models for spontaneous studies about breast cancers, presenting similarities in clinical behavior and molecular pathways of the disease. Thus, analyses of the canine transcriptome can identify deregulated genes and pathways, contributing to the identification of biomarkers and new therapeutic targets, benefiting humans and animals. In this context, this study aimed to determine the transcriptional profile of canine mammary ductal carcinoma and contribute to the clarification of the importance of deregulated molecules in the molecular pathways involved in the disease. Therefore, we used mammary ductal carcinoma tissue samples and non-tumor mammary tissue from the radical mastectomy of six female dogs. Sequencing was performed on the NextSeq-500 System platform. A comparison of carcinoma tissue and normal tissue revealed 633 downregulated and 573 upregulated genes, which were able to differentiate the groups by principal component analysis. Gene ontology analysis indicated that inflammatory, cell differentiation and adhesion, and extracellular matrix maintenance pathways were mainly deregulated in this series. The main differentially expressed genes observed in this research can indicate greater disease aggressiveness and worse prognosis. Finally, the study of the canine transcriptome indicates that it is an excellent model to generate information relevant to oncology in both species.
Secretory Factors from Calcium-Sensing Receptor-Activated SW872 Pre-Adipocytes Induce Cellular Senescence and A Mitochondrial Fragmentation-Mediated Inflammatory Response in HepG2 Cells
Authors: Briones-Suarez Lautaro , Cifuentes Mariana , Bravo-Sagua Roberto
Published: March 2023

Adipose tissue inflammation in obesity has a deleterious impact on organs such as the liver, ultimately leading to their dysfunction. We have previously shown that activation of the calcium-sensing receptor (CaSR) in pre-adipocytes induces TNF-α and IL-1β expression and secretion; however, it is unknown whether these factors promote hepatocyte alterations, particularly promoting cell senescence and/or mitochondrial dysfunction. We generated conditioned medium (CM) from the pre-adipocyte cell line SW872 treated with either vehicle (CMveh) or the CaSR activator cinacalcet 2 µM (CMcin), in the absence or presence of the CaSR inhibitor calhex 231 10 µM (CMcin+cal). HepG2 cells were cultured with these CM for 120 h and then assessed for cell senescence and mitochondrial dysfunction. CMcin-treated cells showed increased SA-β-GAL staining, which was absent in TNF-α- and IL-1β-depleted CM. Compared to CMveh, CMcin arrested cell cycle, increased IL-1β and CCL2 mRNA, and induced p16 and p53 senescence markers, which was prevented by CMcin+cal. Crucial proteins for mitochondrial function, PGC-1α and OPA1, were decreased with CMcin treatment, concomitant with fragmentation of the mitochondrial network and decreased mitochondrial transmembrane potential. We conclude that pro-inflammatory cytokines TNF-α and IL-1β secreted by SW872 cells after CaSR activation promote cell senescence and mitochondrial dysfunction, which is mediated by mitochondrial fragmentation in HepG2 cells and whose effects were reversed with Mdivi-1. This investigation provides new evidence about the deleterious CaSR-induced communication between pre-adipocytes and liver cells, incorporating the mechanisms involved in cellular senescence.
Phylogenetic Analysis of Spliceosome SF3a2 in Different Plant Species
Authors: Tian Yuan , Das Debatosh , Li Min , Song Tao , Yang Jingfang , Liu Yinggao
Published: March 2023

The formation of mature mRNA requires cutting introns and splicing exons. The occurrence of splicing involves the participation of the spliceosome. Common spliceosomes mainly include five snRNPs: U1, U2, U4/U6, and U5. SF3a2, an essential component of spliceosome U2 snRNP, participates in splicing a series of genes. There is no definition of SF3a2 in plants. The paper elaborated on SF3a2s from a series of plants through protein sequence similarity. We constructed the evolutionary relationship of SF3a2s in plants. Moreover, we analyzed the similarities and differences in gene structure, protein structure, the cis-element of the promoter, and expression pattern; we predicted their interacting proteins and constructed their collinearity. We have preliminarily analyzed SF3a2s in plants and clarified the evolutionary relationship between different species; these studies can better serve for in-depth research on the members of the spliceosome in plants.
Identification of a Ubiquinone–Ubiquinol Quinhydrone Complex in Bacterial Photosynthetic Membranes and Isolated Reaction Centers by Time-Resolved Infrared Spectroscopy
Authors: Mezzetti Alberto , Paul Jean-François , Leibl Winfried
Published: March 2023

Ubiquinone redox chemistry is of fundamental importance in biochemistry, notably in bioenergetics. The bi-electronic reduction of ubiquinone to ubiquinol has been widely studied, including by Fourier transform infrared (FTIR) difference spectroscopy, in several systems. In this paper, we have recorded static and time-resolved FTIR difference spectra reflecting light-induced ubiquinone reduction to ubiquinol in bacterial photosynthetic membranes and in detergent-isolated photosynthetic bacterial reaction centers. We found compelling evidence that in both systems under strong light illumination—and also in detergent-isolated reaction centers after two saturating flashes—a ubiquinone–ubiquinol charge-transfer quinhydrone complex, characterized by a characteristic band at ~1565 cm−1, can be formed. Quantum chemistry calculations confirmed that such a band is due to formation of a quinhydrone complex. We propose that the formation of such a complex takes place when Q and QH2 are forced, by spatial constraints, to share a common limited space as, for instance, in detergent micelles, or when an incoming quinone from the pool meets, in the channel for quinone/quinol exchange at the QB site, a quinol coming out. This latter situation can take place both in isolated and membrane bound reaction centers Possible consequences of the formation of this charge-transfer complex under physiological conditions are discussed.
A Sulfur Containing Melanogenesis Substrate, N-Pr-4-S-CAP as a Potential Source for Selective Chemoimmunotherapy of Malignant Melanoma
Authors: Tamura Yasuaki , Ito Akira , Wakamatsu Kazumasa , Torigoe Toshihiko , Honda Hiroyuki , Ito Shosuke , Jimbow Kowichi
Published: March 2023

N-propionyl-4-S-cysteaminylphenol (N-Pr-4-S-CAP) is a substrate for tyrosinase, which is a melanin biosynthesis enzyme and has been shown to be selectively incorporated into melanoma cells. It was found to cause selective cytotoxicity against melanocytes and melanoma cells after selective incorporation, resulting in the induction of anti-melanoma immunity. However, the underlying mechanisms for the induction of anti-melanoma immunity remain unclear. This study aimed to elucidate the cellular mechanism for the induction of anti-melanoma immunity and clarify whether N-Pr-4-S-CAP administration could be a new immunotherapeutic approach against melanoma, including local recurrence and distant metastasis. A T cell depletion assay was used for the identification of the effector cells responsible for N-Pr-4-S-CAP-mediated anti-melanoma immunity. A cross-presentation assay was carried out by using N-Pr-4-S-CAP-treated B16-OVA melanoma-loaded bone marrow-derived dendritic cells (BMDCs) and OVA-specific T cells. Administration of N-Pr-4-S-CAP induced CD8+ T cell-dependent anti-melanoma immunity and inhibited the growth of challenged B16F1 melanoma cells, indicating that the administration of N-Pr-4-S-CAP can be a prophylactic therapy against recurrence and metastasis of melanoma. Moreover, intratumoral injection of N-Pr-4-S-CAP in combination with BMDCs augmented the tumor growth inhibition when compared with administration of N-Pr-4-S-CAP alone. BMDCs cross-presented a melanoma-specific antigen to CD8+ T cells through N-Pr-4-S-CAP-mediated melanoma cell death. Combination therapy using N-Pr-4-S-CAP and BMDCs elicited a superior anti-melanoma effect. These results suggest that the administration of N-Pr-4-S-CAP could be a new strategy for the prevention of local recurrence and distant metastasis of melanoma.