bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impacts of Water Pollution Emissions on Public Health in 30 Provinces of China
Authors: Shi Zhen , Qin Shijiong , Zhang Chenjun , Chiu Yung-ho , Zhang Lina
Published: May 2020

China’s economy in recent decades has developed at a very rapid speed, as evidenced by its GDP jumping to second place in the world. Although utilization of domestic water resources has helped spur economic development, sewage discharge as an undesirable output has unfortunately caused many negative effects on human health, causing concern from all walks of life. Therefore, governments in China at all levels are committed to urban sewage treatment policies in order to reduce the negative impact of water pollution on society. While most existing studies have targeted the macro-level modes of economic development and environmental pollution, their selection of research objects is too narrow by failing to adequately consider China's water pollution and the consequential national health crisis. This study takes cities in 30 provinces of China as the research objects and applies various influencing factors of urban wastewater treatment and health (as two stages) to the modified two-stage dynamic Slacks-Based Measures (SBM) Data Envelopment Analysis (DEA) model. The results reveal that the overall efficiency of each province is increasing and that the efficiency of the wastewater treatment stage is greater, thus contributing to overall efficiency. Conversely, the health stage’s efficiency is far lower than the wastewater treatment stage’s efficiency, which has a notably adverse effect on overall efficiency. In addition, most input-output variables need much improvement. Based on the findings herein, we offer specific suggestions to each province for improving sewage treatment capacity, the level of medical care, and the quality of national health.
Multiyear Links between Water Chemistry, Algal Chlorophyll, Drought-Flood Regime, and Nutrient Enrichment in a Morphologically Complex Reservoir
Authors: HaRa Jang , Atique Usman , An Kwang-Guk
Published: May 2020

This investigation targeted the largest morphologically complex reservoir (Soyang) in South Korea during 1992–2013. It is a prominent source of domestic water supply, irrigation, flood control, and hydroelectric power generation. Therefore, this investigation focused on regional- to global-scale applications. We revealed the empirical links between chlorophyll (Chl-a) and total nitrogen (TN) and total phosphorus (TP), the impact of the monsoon regime on nutrients, and flood and drought regime. Further, we investigated the trophic status dynamics, tendencies of water chemistry factors, and valuation of zonal water chemistry by the application of a modified multimetric water pollution index (WPI). The physicochemical indicators illustrated significant disparities among the Lacustrine (Lz), Transition (Tz), and Riverine (Rz) zones. The solid contents (TSS) displayed a significant increase in the lake zones in the order of Lz (4.58 ± 13.7 mg/L), Tz (6.16 ± 16.2 mg/L), and Rz (7.38 ± 18.9 mg/L). However, TP and allied chemical species revealed an inverse relationship with the TN:TP ambient ratios. Nevertheless, Chl-a displayed sharp interzonal fluctuations from the Lz (2.90 ± 3.29 µg/L) to Tz (4.61 ± 4.98 µg/L). The seasonal deviations, however, exposed divergent heterogeneities among the TSS, TN, TP, and Chl-a. The regression plot between the observed and predicted Chl-a in the Soyang reservoir displayed a very strong relationship (R2 = 0.997). The seasonal and interannual variations of trophic status displayed a higher impact of precipitation, particularly in the case of TP and Chl-a. The flood years indicated phosphorus limitations, while drought years alluded to the non-algal light limitations (biogenic turbidity). Water temperature (WT), dissolved oxygen (DO), biological oxygen demand (BOD), TSS, TP, and Chl-a displayed decreasing trends in the ambient water. In contrast, pH, chemical oxygen demand (COD), electrical conductivity (EC), and TN displayed increasing tendencies by the application Mann–Kendall trend analysis. The WPI outcomes designated Lz with excellent water quality while Tz an Rz indicated good water quality. It also indicated impending sedimentation tendencies in the Rz. In conclusion, our findings indicated fluctuating rainfall patterns (drought and flood conditions) that significantly impacted the Soyang reservoir water quality, flood and drought severity, and trophic status of the reservoir. This study highlights the requirements of further studies to substantiate the drought and flood dynamics and their impacts on nutrients and overall water quality status.
Gender Differences in Contraceptive Self-Efficacy: A Cross-Sectional Study of South Korean College Students
Authors: Jun Eun-Young , Oh Hyunjin
Published: May 2020

Purpose: Healthy sexuality is an important issue in the transition to adulthood. To maintain healthy sexuality, contraceptive self-efficacy could be the most significant predictor of safe sexuality. The purpose of this study was to explore the relationship between gender roles and the healthy sexuality of South Korean college students. Method: A cross-sectional study through self-report questionnaires was administered to college students in South Korea. In total, 499 students completed the demographic information questionnaire, the Sexual Attitudes Scale, Sexual Autonomy Scale, and Condom Self-Efficacy Scale. Results: A multiple regression analysis indicated that the contraceptive self-efficacy of male students was predicted by their junior year and sexual autonomy, while that of the females was predicted by their senior year and sexual autonomy. Conclusion: Sexual education focusing on sexual autonomy should be provided for the safe and healthy sexual expression of college students.
Workplace Aggression and Burnout in Nursing—The Moderating Role of Follow-Up Counseling
Authors: Vincent-Höper Sylvie , Stein Maie , Nienhaus Albert , Schablon Anja
Published: May 2020

The aim of this study is to obtain a better understanding of the association between the frequency of nurses’ exposure to workplace aggression from patients and their levels of burnout. In particular, we seek to shed light on the role of the availability of follow-up counseling in organizations after critical incidents in mitigating the adverse relationships between physical and verbal aggression and nurses’ burnout. A total of 582 nurses reported how frequently they had experienced physical and verbal aggression from patients in the last 12 months and whether they had the opportunity to receive follow-up counseling in their organization. In addition, nurses rated the extent to which they experienced each of the three dimensions of burnout (i.e., emotional exhaustion, depersonalization, and personal accomplishment). The results showed that both physical and verbal aggression were substantially related to the burnout dimensions. Furthermore, we found that the availability of follow-up counseling in organizations attenuated the relationships between physical aggression and all three burnout dimensions. While we found that the availability of follow-up counseling moderated the relationship between verbal aggression depersonalization, the moderating effects were not significant for emotional exhaustion and personal accomplishment. The findings indicate that the availability of follow-up counseling might help minimize the adverse impact of exposure to aggression from patients on nurses’ mental health.
The Correlation between Hemoglobin A1c (HbA1c) and Hyperreflective Dots (HRD) in Diabetic Patients
Authors: Wong Bing Seng , Sharanjeet-Kaur Sharanjeet , Ngah Nor Fariza , Sawri Rajan Rajasudha
Published: May 2020

Hyperreflective dots (HRD) are activated retinal microglial cells induced by retinal inflammation in diabetic patients. This study was conducted to compare the HRD count of normal and diabetic subjects; to determine the correlation between hemoglobin A1c (HbA1c) levels and HRD count; to determine HbA1c cut-off levels for the appearance of HRD in diabetic patients. A cross-sectional study was conducted among normal and diabetic patients. Fundus photos, SD-OCT images and HbA1c levels were taken. A total of 25 normal subjects, 32 diabetics without retinopathy and 26 mild-to-moderate nonproliferative diabetic retinopathy (NPDR) diabetics were recruited. There was a statistically significant difference between the mean count of HRD among the normal group, the diabetic without retinopathy group and the mild-to-moderate NPRD group. The mean HRD count in the inner retina layer was significantly higher compared to the outer retina layer. There was a significant linear relationship between the HbA1c levels and HRD count. Using the receiver operating curve, the HbA1c level of 5.4% was chosen as the cut-off point for the appearance of HRD. The positive linear correlation between the HbA1c levels and the appearance of HRD may indicate that hyperglycemia could activate retina microglial cells in diabetic patients.
Ageism, Healthy Life Expectancy and Population Ageing: How Are They Related?
Authors: Officer Alana , Thiyagarajan Jotheeswaran Amuthavalli , Schneiders Mira Leonie , Nash Paul , de la Fuente-Núñez Vânia
Published: May 2020

Evidence shows that ageism negatively impacts the health of older adults. However, estimates of its prevalence are lacking. This study aimed to estimate the global prevalence of ageism towards older adults and to explore possible explanatory factors. Data were included from 57 countries that took part in Wave 6 of the World Values Survey. Multilevel Latent Class Analysis was performed to identify distinct classes of individuals and countries. Individuals were classified as having high, moderate or low ageist attitudes; and countries as being highly, moderately or minimally ageist, by aggregating individual responses. Individual-level (age, sex, education and wealth) and contextual-level factors (healthy life expectancy, population health status and proportion of the population aged over 60 years) were examined as potential explanatory factors in multinomial logistic regression. From the 83,034 participants included, 44%, 32% and 24% were classified as having low, moderate and high ageist attitudes, respectively. From the 57 countries, 34 were classified as moderately or highly ageist. The likelihood of an individual or a country being ageist was significantly reduced by increases in healthy life expectancy and the proportion of older people within a country. Certain personal characteristics—younger age, being male and having lower education—were significantly associated with an increased probability of an individual having high ageist attitudes. At least one in every two people included in this study had moderate or high ageist attitudes. Despite the issue’s magnitude and negative health impacts, ageism remains a neglected global health issue.
A Nationwide Survey of Psychological Distress among Italian People during the COVID-19 Pandemic: Immediate Psychological Responses and Associated Factors
Authors: Mazza Cristina , Ricci Eleonora , Biondi Silvia , Colasanti Marco , Ferracuti Stefano , Napoli Christian , Roma Paolo
Published: May 2020

The uncontrolled spread of the coronavirus disease 2019 (COVID-19) has called for unprecedented measures, to the extent that the Italian government has imposed a quarantine on the entire country. Quarantine has a huge impact and can cause considerable psychological strain. The present study aims to establish the prevalence of psychiatric symptoms and identify risk and protective factors for psychological distress in the general population. An online survey was administered from 18–22 March 2020 to 2766 participants. Multivariate ordinal logistic regression models were constructed to examine the associations between sociodemographic variables; personality traits; depression, anxiety, and stress. Female gender, negative affect, and detachment were associated with higher levels of depression, anxiety, and stress. Having an acquaintance infected was associated with increased levels of both depression and stress, whereas a history of stressful situations and medical problems was associated with higher levels of depression and anxiety. Finally, those with a family member infected and young person who had to work outside their domicile presented higher levels of anxiety and stress, respectively. This epidemiological picture is an important benchmark for identifying persons at greater risk of suffering from psychological distress and the results are useful for tailoring psychological interventions targeting the post-traumatic nature of the distress.
Epidemiology of Surgical Site Infections Considering the NHSN Standardized Infection Ratio in Hip and Knee Arthroplasties
Authors: Słowik Róża , Kołpa Małgorzata , Wałaszek Marta , Różańska Anna , Jagiencarz-Starzec Barbara , Zieńczuk Witold , Kawik Łukasz , Wolak Zdzisław , Wójkowska-Mach Jadwiga
Published: May 2020

Introduction Surgical site infections (SSIs) are a predominant form of hospital-acquired infections in surgical wards. The objective of the study was analysis of the incidence of SSI in, both primary and revision, hip and knee arthroplasties. Material and methods: The study was conducted in 2012–2018 in a Trauma and Orthopedics Ward in Tarnów according to the methodology of the Healthcare-Associated Infections Surveillance Network (HAI-Net), European Centre for Disease Prevention and Control (ECDC). Results: The surveillance comprised 2340 surgery patients, including: 1756 Hip Arthroplasties (HPRO) and 584 Knee Arthroplasties (KPRO). In the group of patients under study, 37 cases of SSI were detected, including: 26 cases of SSI after HPRO and 11 cases in KPRO. The average incidence of SSI amounted to 1.6% (1.5% HPRO and 1.9% KPRO) and in-hospital incidence density rates were 1.23 and 1.53 per 1000 patient-days, respectively. Median age of surgical patients in both HPRO and KPRO was 70 years. Women were undergoing arthroplasty surgery more often than men, HPRO (p < 0.05) and KPRO (p < 0.001). Patients with SSI stayed in the ward longer (SSI-HPRO, p < 0.001) (SSI-KPRO p < 0.01). In KPRO operations, the incidence of SSI was higher than expected, calculated according to the Standardized Infection Ratio (SIR). The most common etiologic agents isolated from SSIs in both HPRO and KPRO were coagulase-negative staphylococci. Conclusions: Establishing a thorough surveillance of hospital-acquired infections that takes into consideration epidemiological indicators is indispensable to properly assess the epidemiological situation in the ward. The optimal solution is to carry out long-term and multi-center surveillance in the framework of a uniform program, however, even results of single-center studies provide valuable data indicating challenges and needs in improving patient safety.
Impact of Wind Speed on Response of Diffusion-Type Radon-Thoron Detectors to Thoron
Authors: Omori Yasutaka , Tamakuma Yuki , Nugraha Eka Djatnika , Suzuki Takahito , Saputra Miki Arian , Hosoda Masahiro , Tokonami Shinji
Published: May 2020

Air exchange through a porous medium depends partly on a pressure gradient induced in it, i.e., air-flow conditions of the outer air. Consequently, response of diffusion-type detectors to radon and thoron may vary with air-flow conditions surrounding the detectors. This effect may be significant for thoron measurement because thoron has a shorter half-life than radon. The present study examined response of diffusion-type detectors (RADUETs and one AlphaGUARD) to thoron with respect to wind speed using a thoron calibration chamber. Response of RADUETs to thoron increased with wind speed. Response of the AlphaGUARD increased with wind speed, but it became constant at a high wind speed. Different response trends to thoron between the RADUETs and the AlphaGUARD could be qualitatively explained by flow states induced by the pressure gradient in the filter or the sponge of these detectors. For RADUETs, laminar (Darcy) flow was induced in the sponge in the examined wind speed range, which meant that thoron entry into the detector increased with wind speed. For the AlphaGUARD, laminar flow was induced in the filter in the low wind speed range, whereas flow was changed to turbulent (non-Darcy) flow at a high wind speed for which thoron entry into the detector did not depend on wind speed.
Knowledge, Attitude and Practices (KAP) towards Diet and Health among International Students in Dublin: A Cross-Sectional Study
Authors: Liu Xiyao , Chen Haoyue , Zhou Qianling , Zhang Huifeng , Asawasirisap Phensiri , Kearney John
Published: May 2020

International students may have difficulties in dietary acculturation. This study aimed to evaluate the knowledge, attitude and practices (KAP) of diet and health during the acculturation of international students. A cross-sectional survey was conducted among a convenience sample of 473 international students in Dublin. Knowledge, attitude and practices towards diet and health were evaluated by a questionnaire with open- and closed-ended questions. It was found that 45.3% of participants had a broad concept of a healthy diet, while few knew its specific contents. Furthermore, 75.3% of participants could explain the term functional food, and among them, 62.1% knew the appropriate definition of functional food. Participants who perceived their health very good and excellent were more likely to believe that their health status was determined by their own control. The consumption rate of functional food varied among regions and South and Central America students had the highest usage rate (44.5%) and Asian students had the highest daily usage rate (52.7%). Participants who were younger, single, from African and South and Central American countries, or who were in Ireland for less than one year were more likely to report dietary change after immigration. In conclusion, insufficient knowledge and self-perception towards diet and health as well as unhealthily dietary changes exist among international students living in Dublin.
Age-Specific Breast Density Changes in Taiwanese Women: A Cross-Sectional Study
Authors: Liao Yu-San , Zhang Jia-Yu , Hsu Yuan-Chi , Hong Min-Xuan , Lee Li-Wen
Published: May 2020

Breast density is a risk factor for breast cancer. This study explored distribution of mammographic density quantitatively and qualitatively in a wide age range of Taiwanese women. Subjects with negative and benign mammographic findings were included. According to the Breast Imaging Reporting and Data System, the proportion of extremely dense breasts declined from 58.0% in women < 30 years to 1.9% in women > 74 years. More than 80% of mammograms in women < 55 years old were classified as extremely or heterogeneously dense, while the proportion of dense breasts was still high in women aged 60–64 years (59.3%). The absolute dense area of the breast declined from 35.8% in women < 30 years to 18.5% in women > 74 years. The correlation between breast density and age was significant, with and without controlling for the effect of body composition (p < 0.001), implying that the relationship between breast density and age was not wholly related to body composition. In conclusion, the higher breast density in Taiwanese women aged 60–64 years was comparable to that of Western women aged 40–44 years in the literature. This suggests that breast cancer screening using mammography may be more challenging for Asian women than for Western women of the same age.
Are Your Eyes “on the Road”? Findings from the 2019 National Study on Vision and Driving Safety in Spain
Authors: Lijarcio Ignacio , Useche Sergio A. , Llamazares Javier , Montoro Luis
Published: May 2020

Background: Vision is an undisputable contributor to the explanation of many human-factor related traffic crashes happening every day. The Inland Transport Committee (ITC), the United Nations regulatory platform, included on 1st April 2020 special action on the vision of road users inside the ITC Recommendations for Enhancing Road Safety Systems. The results of this wide-scale study on drivers’ vision health conducted in Spain perfectly illustrates the need of global action and its potential impact on the public health figures and the burden of potentially preventable traffic causalities. Objective: The aim of this study was to assess three key visual health issues (i.e., visual acuity, visual field campimetry and glare recovery) among Spanish drivers, in order to formulate implications and possible guidelines to enhance road safety. Methods: This cross-sectional study examined the visual health of a representative sample of 3249 drivers (70% females and 30% males) with a mean age of 41 (SD = 13) years, gathered from all the 17 autonomous communities of Spain. Results: The tests performed allowed to determine that 15% of Spanish drivers have a poor photopic vision, while 38% of them present an inadequate mesopic vision. Further, 23% of drivers have deficiencies in peripheric visual field campimetry, and the average time for full-vision recovery after a 10-s glare was 27 s. Sex, age and driver type (professional vs. non-professional) differences were found for the study variables. Conclusions: The findings of this study support the idea that certain demographic-based population groups of drivers present several unaddressed deficiencies and impairments in visual health. Overall an estimated 29.5% of Spanish drivers present visual issues, that need to be attended in order to enhance the prevention of driving crashes and the road safety of all road users.
Emerging from the Shadows: Intrinsic and Extrinsic Factors Facing Community Health Workers in Western Cape, South Africa
Authors: Majee Wilson , Schopp Laura , Johnson Levona , Anakwe Adaobi , Rhoda Anthea , Frantz Jose
Published: May 2020

Community health workers (CHWs) have been identified as a key component of the health workforce in South Africa. However, the efficacy of CHW programs continues to be limited by a poor understanding of facilitators and barriers to CHW engagement. This study explores intrinsic and extrinsic factors that CHWs face. We conducted in-depth interviews with 20 CHWs in order to understand the challenges they may face as they implement their duties linked to the primary health care strategy in the Western Cape, South Africa. All interviews were audiotaped, transcribed verbatim, coded and analyzed using NVivo 12. Drawing on narratives of CHWs, we illustrate the complex issues surrounding CHW outreach in poor rural communities. The CHWs identified five key areas of challenges with respect to personal health, gender issues, poor community understanding of CHWs roles, environmental challenges and lack of patient adherence. These all hinder the ability of CHWs to meet their personal and familial needs, as well as those of the community members they support. There is a need to address the intrinsic needs of CHWs in order to ensure their emotional and physical well-being, as well as a need to create an awareness of the roles of CHWs.
Youth Experiences with Social Norms Feedback: Qualitative Findings from The Drug Prevention Trial the GOOD Life
Authors: Stock Christiane , Lavasani Kjær Satayesh , Rasmussen Birthe , Vallentin-Holbech Lotte
Published: May 2020

Background: Normative feedback is an intervention strategy commonly used in drug prevention programmes. This study collected process evaluation data about how programme recipients engage with social norms (SN) feedback in The GOOD Life intervention and how they experience it. Methods: Eight focus group interviews were conducted with a total of 44 adolescents (pupils aged 14–16 years) who have participated in the social-norms-based intervention The GOOD Life. The interviews focused on three topics: (1) interest in and impact of the intervention; (2) perception of the intervention elements; and (3) suggestions for improvement of The GOOD Life. They were transcribed and analysed with content analysis. Results: The analysis revealed that The GOOD Life motivated pupils to re-evaluate their own drug use behaviour and overall met their interest regarding receiving engaging and non-moral forms of drug prevention programmes. While pupils perceived the normative feedback session in the classroom and the posters with SN messages as positive, stimulating and surprising, the web-based application with SN feedback was rarely used and less positively evaluated. Anonymity and confidentiality were regarded as essential to provide honest answers in the poll. The pupils suggested even more variety in ways to engage them and to use more gaming elements. Conclusions: SN feedback was well perceived by adolescents. The intervention met their interest and needs and was able to achieve the intended impact of challenging norm perceptions. Anonymity and confidentiality are key in order to build trust and engage adolescents in the intervention.
Financial Inclusion in Ethiopia: Is It on the Right Track?
Authors: Berhanu Lakew Tekeste , Azadi Hossein
Published: May 2020

It is important to evaluate the impact of Ethiopia’s financial inclusion strategy since it has been launched in 2014. Accordingly, this paper assesses the extent to which the target has been met. The main aim of this study is to measure the success or failure of Ethiopia’s financial inclusion in comparison with other countries in East Africa. Using secondary data, this study revealed that Ethiopia’s financial inclusion is not as successful as other East African countries. This study also found that Ethiopians prefer informal saving clubs rather than formal financial organs. This preference, combined with unemployment and low income, is the barrier to the financial inclusion strategy. Based on the findings, identifying and addressing root causes should be done by removing distance, cost, credit, and documentation barriers. Moreover, the findings showed that access to public transit can also expand the reach of formal financial institutions by encouraging more people to physically access financial institutions. This study recommended access to formal financial organs as a core to financial institutions. Access to formal financial organs should be boosted through increasing financial institutions. Educating individuals about their financial circumstances were also recommended so that people can increase their formal saving uptake. This paper also recommended that the government develop regulatory guidelines for the functioning of financial institutions. The main outcome, therefore, is that financial institutions could be more transparent and predictable, reduce costs, and simplify the rules for entering the market.
Isolation and Characterization of Two Klebsiella pneumoniae Phages Encoding Divergent Depolymerases
Authors: Domingo-Calap Pilar , Beamud Beatriz , Mora-Quilis Lucas , González-Candelas Fernando , Sanjuán Rafael
Published: May 2020

The emergence of multidrug-resistant bacteria is a major global health concern. The search for new therapies has brought bacteriophages into the spotlight, and new phages are being described as possible therapeutic agents. Among the bacteria that are most extensively resistant to current antibiotics is Klebsiella pneumoniae, whose hypervariable extracellular capsule makes treatment particularly difficult. Here, we describe two new K. pneumoniae phages, πVLC5 and πVLC6, isolated from environmental samples. These phages belong to the genus Drulisvirus within the family Podoviridae. Both phages encode a similar tail spike protein with putative depolymerase activity, which is shared among other related phages and probably determines their ability to specifically infect K. pneumoniae capsular types K22 and K37. In addition, we found that phage πVLC6 also infects capsular type K13 and is capable of striping the capsules of K. pneumoniae KL2 and KL3, although the phage was not infectious in these two strains. Genome sequence analysis suggested that the extended tropism of phage πVLC6 is conferred by a second, divergent depolymerase. Phage πVLC5 encodes yet another putative depolymerase, but we found no activity of this phage against capsular types other than K22 and K37, after testing a panel of 77 reference strains. Overall, our results confirm that most phages productively infected one or few Klebsiella capsular types. This constitutes an important challenge for clinical applications.
Convolutional Neural Network Can Recognize Drug Resistance of Single Cancer Cells
Authors: Yanagisawa Kiminori , Toratani Masayasu , Asai Ayumu , Konno Masamitsu , Niioka Hirohiko , Mizushima Tsunekazu , Satoh Taroh , Miyake Jun , Ogawa Kazuhiko , Vecchione Andrea , Doki Yuichiro , Eguchi Hidetoshi , Ishii Hideshi
Published: May 2020

It is known that single or isolated tumor cells enter cancer patients’ circulatory systems. These circulating tumor cells (CTCs) are thought to be an effective tool for diagnosing cancer malignancy. However, handling CTC samples and evaluating CTC sequence analysis results are challenging. Recently, the convolutional neural network (CNN) model, a type of deep learning model, has been increasingly adopted for medical image analyses. However, it is controversial whether cell characteristics can be identified at the single-cell level by using machine learning methods. This study intends to verify whether an AI system could classify the sensitivity of anticancer drugs, based on cell morphology during culture. We constructed a CNN based on the VGG16 model that could predict the efficiency of antitumor drugs at the single-cell level. The machine learning revealed that our model could identify the effects of antitumor drugs with ~0.80 accuracies. Our results show that, in the future, realizing precision medicine to identify effective antitumor drugs for individual patients may be possible by extracting CTCs from blood and performing classification by using an AI system.
Correction: Lin, K.-H., et al. Molecular Functions of Thyroid Hormone Signaling in Regulation of Cancer Progression and Anti-Apoptosis. Int. J. Mol. Sci., 2019, 20, 4986
Authors: Liu Yu-Chin , Yeh Chau-Ting , Lin Kwang-Huei
Published: May 2020

The authors wish to make the following corrections to this paper [...]
Dietary AhR Ligands Regulate AhRR Expression in Intestinal Immune Cells and Intestinal Microbiota Composition
Authors: Schanz Oliver , Chijiiwa Rieka , Cengiz Sevgi Can , Majlesain Yasmin , Weighardt Heike , Takeyama Haruko , Förster Irmgard
Published: May 2020

A diet rich in vegetables and fruit is generally considered healthy because of a high content of phytochemicals, vitamins, and fiber. The phytochemical indole-3-carbinol (I3C), a derivative of glucobrassicin, is sold as a dietary supplement promising diverse health benefits. I3C metabolites act as ligands of the aryl hydrocarbon receptor (AhR), an important sensor for environmental polyaromatic chemicals. Here, we investigated how dietary AhR ligand supplementation influences AhR target gene expression and intestinal microbiota composition. For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet. AhRR expression in intestinal immune cells was mainly driven by dietary AhR ligands and was independent of microbial metabolites. A lack of dietary AhR ligands caused enhanced susceptibility to dextran sodium sulfate (DSS)-induced colitis and correlated with the expansion of Enterobacteriaceae, whereas Clostridiales, Muribaculaceae, and Rikenellaceae were strongly reduced. I3C supplementation largely reverted this effect. Comparison of I3C-induced changes in microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice revealed both AhR-dependent and -independent alterations in the microbiome. Overall, our study demonstrates that dietary AhR ligand supplementation has a profound influence on Ahrr expression in intestinal immune cells as well as microbiota composition.
The Role of Sclerostin in Bone and Ectopic Calcification
Authors: De Maré Annelies , D’Haese Patrick C. , Verhulst Anja
Published: May 2020

Sclerostin, a 22-kDa glycoprotein that is mainly secreted by the osteocytes, is a soluble inhibitor of canonical Wnt signaling. Therefore, when present at increased concentrations, it leads to an increased bone resorption and decreased bone formation. Serum sclerostin levels are known to be increased in the elderly and in patients with chronic kidney disease. In these patient populations, there is a high incidence of ectopic cardiovascular calcification. These calcifications are strongly associated with cardiovascular morbidity and mortality. Although data are still controversial, it is likely that there is a link between ectopic calcification and serum sclerostin levels. The main question, however, remains whether sclerostin exerts either a protective or deleterious role in the ectopic calcification process.
Ultraviolet Irradiation Enhances the Microbicidal Activity of Silver Nanoparticles by Hydroxyl Radicals
Authors: Nakamura Shingo , Ando Naoko , Sato Masahiro , Ishihara Masayuki
Published: May 2020

It is known that silver has microbicidal qualities; even at a low concentration, silver is active against many kinds of bacteria. Silver nanoparticles (AgNPs) have been extensively studied for a wide range of applications. Alternately, the toxicity of silver to human cells is considerably lower than that to bacteria. Recent studies have shown that AgNPs also have antiviral activity. We found that large amounts of hydroxyl radicals—highly reactive molecular species—are generated when AgNPs are irradiated with ultraviolet (UV) radiation with a wavelength of 365 nm, classified as ultraviolet A (UVA). In this study, we used electron spin resonance direct detection to confirm that UV irradiation of AgNPs produced rapid generation of hydroxyl radicals. As hydroxyl radicals are known to degrade bacteria, viruses, and some chemicals, the enhancement of the microbicidal activity of AgNPs by UV radiation could be valuable for the protection of healthcare workers and the prevention of the spread of infectious diseases.
Effects of Strontium-Doped β-Tricalcium Scaffold on Longitudinal Nuclear Factor-Kappa Beta and Vascular Endothelial Growth Factor Receptor-2 Promoter Activities during Healing in a Murine Critical-Size Bone Defect Model
Authors: Tohidnezhad Mersedeh , Kubo Yusuke , Lichte Philipp , Heigl Tobias , Roch Diana , Barahmand Pour Nazanin , Bergmann Christian , Sönmez Tolga Taha , Hock Jennifer Vanessa Phi , Fragoulis Athanassios , Gremse Felix , Rosenhain Stefanie , Slowik Alexander , Bienert Michaela , Kweider Nisreen , Wruck Christoph Jan , Jahr Holger , Hildebrand Frank , Pape Hans Christoph , Neuß Sabine , Fischer Horst , Pufe Thomas
Published: May 2020

It was hypothesized that strontium (Sr)-doped β-tricalcium phosphate (TCP)-based scaffolds have a positive effect on the regeneration of large bone defects (LBD). Readouts in our mice models were nuclear factor-kappa beta (NF-κB) activity and vascular endothelial growth factor receptor-2 (VEGFR-2) promoter activity during the healing process. A 2-mm critical-size femoral fracture was performed in transgenic NF-κB- and VEGFR-2-luciferase reporter mice. The fracture was filled with a 3D-printed β-TCP scaffold with or without Sr. A bioluminescence in-vivo imaging system was used to sequentially investigate NF-κB and VEGFR-2 expression for two months. After sacrifice, soft and osseous tissue formation in the fracture sites was histologically examined. NF-κB activity increased in the β-TCP + Sr group in the latter stage (day 40–60). VEGFR-2 activity increased in the + Sr group from days 0–15 but decreased and showed significantly less activity than the β-TCP and non-scaffold groups from days 40–60. The new bone formation and soft tissue formation in the + Sr group were significantly higher than in the β-TCP group, whereas the percentage of osseous tissue formation in the β-TCP group was significantly higher than in the β-TCP + Sr group. We analyzed longitudinal VEGFR-2 promoter activity and NF-κB activity profiles, as respective agents of angiogenesis and inflammation, during LBD healing. The extended inflammation phase and eventually more rapid resorption of scaffold caused by the addition of strontium accelerates temporary bridging of the fracture gaps. This finding has the potential to inform an improved treatment strategy for patients who suffer from osteoporosis.
Enhanced Integrin Activation of PLD2-Deficient Platelets Accelerates Inflammation after Myocardial Infarction
Authors: Klose Aglaia Maria , Klier Meike , Gorressen Simone , Elvers Margitta
Published: May 2020

Background: Phospholipase (PL)D1 is crucial for integrin αIIbβ3 activation of platelets in arterial thrombosis and TNF-α-mediated inflammation and TGF-β-mediated collagen scar formation after myocardial infarction (MI) in mice. Enzymatic activity of PLD is not responsible for PLD-mediated TNF-α signaling and myocardial healing. The impact of PLD2 in ischemia reperfusion injury is unknown. Methods: PLD2-deficient mice underwent myocardial ischemia and reperfusion (I/R). Results: Enhanced integrin αIIbβ3 activation of platelets resulted in elevated interleukin (IL)-6 release from endothelial cells in vitro and enhanced IL-6 plasma levels after MI in PLD2-deficient mice. This was accompanied by enhanced migration of inflammatory cells into the infarct border zone and reduced TGF-β plasma levels after 72 h that might account for enhanced inflammation in PLD2-deficient mice. In contrast to PLD1, TNF-α signaling, infarct size and cardiac function 24 h after I/R were not altered when PLD2 was deleted. Furthermore, TGF-β plasma levels, scar formation and heart function were comparable between PLD2-deficient and control mice 21 days post MI. Conclusions: The present study contributes to our understanding about the role of PLD isoforms and altered platelet signaling in the process of myocardial I/R injury.
The Histological, Effectoromic, and Transcriptomic Analyses of Solanum pinnatisectum Reveal an Upregulation of Multiple NBS-LRR Genes Suppressing Phytophthora infestans Infection
Authors: Gu Biao , Cao Xiaoli , Zhou Xiaoli , Chen Zhaodan , Wang Qinhu , Liu Wei , Chen Qin , Zhao Hua
Published: May 2020

Utilization of disease resistance components from wild potatoes is a promising and sustainable approach to control Phytophthora blight. Here, we combined avirulence (Avr) genes screen with RNA-seq analysis to discover the potential mechanism of resistance in Mexican wild potato species, Solanum pinnatisectum. Histological characterization displayed that hyphal expansion was significantly restricted in epidermal cells and mesophyll cell death was predominant, indicating that a typical defense response was initiated in S. pinnatisectum. Inoculation of S. pinnatisectum with diverse Phytophthora infestans isolates showed distinct resistance patterns, suggesting that S. pinnatisectum has complex genetic resistance to most of the prevalent races of P. infestans in northwestern China. Further analysis by Avr gene screens and comparative transcriptomic profiling revealed the presence and upregulation of multiple plant NBS-LRR genes corresponding to biotic stresses. Six NBS-LRR alleles of R1, R2, R3a, R3b, R4, and Rpi-smira2 were detected, and over 60% of the 112 detected NLR proteins were significantly induced in S. pinnatisectum. On the contrary, despite the expression of the Rpi-blb1, Rpi-vnt1, and Rpi-smira1 alleles, fewer NLR proteins were expressed in susceptible Solanum cardophyllum. Thus, the enriched NLR genes in S. pinnatisectum make it an ideal genetic resource for the discovery and deployment of resistance genes for potato breeding.
The Impact of Transcription Factor Prospero Homeobox 1 on the Regulation of Thyroid Cancer Malignancy
Authors: Rudzińska Magdalena , Czarnocka Barbara
Published: May 2020

Transcription factor Prospero homeobox 1 (PROX1) is continuously expressed in the lymphatic endothelial cells, playing an essential role in their differentiation. Many reports have shown that PROX1 is implicated in cancer development and acts as an oncoprotein or suppressor in a tissue-dependent manner. Additionally, the PROX1 expression in many types of tumors has prognostic significance and is associated with patient outcomes. In our previous experimental studies, we showed that PROX1 is present in the thyroid cancer (THC) cells of different origins and has a high impact on follicular thyroid cancer (FTC) phenotypes, regulating migration, invasion, focal adhesion, cytoskeleton reorganization, and angiogenesis. Herein, we discuss the PROX1 transcript and protein structures, the expression pattern of PROX1 in THC specimens, and its epigenetic regulation. Next, we emphasize the biological processes and genes regulated by PROX1 in CGTH-W-1 cells, derived from squamous cell carcinoma of the thyroid gland. Finally, we discuss the interaction of PROX1 with other lymphatic factors. In our review, we aimed to highlight the importance of vascular molecules in cancer development and provide an update on the functionality of PROX1 in THC biology regulation.
Short-Term Physiological Effects of a Very Low-Calorie Ketogenic Diet: Effects on Adiponectin Levels and Inflammatory States
Authors: Monda Vincenzo , Polito Rita , Lovino Annarita , Finaldi Antonio , Valenzano Anna , Nigro Ersilia , Corso Gaetano , Sessa Francesco , Asmundo Alessio , Di Nunno Nunzio , Cibelli Giuseppe , Messina Giovanni
Published: May 2020

Adipose tissue is a multifunctional organ involved in many physiological and metabolic processes through the production of adipokines and, in particular, adiponectin. Caloric restriction is one of the most important strategies against obesity today. The very low-calorie ketogenic diet (VLCKD) represents a type of caloric restriction with very or extremely low daily food energy consumption. This study aimed to investigate the physiological effects of a VLCKD on anthropometric and biochemical parameters such as adiponectin levels, as well as analyzing oligomeric profiles and cytokine serum levels in obese subjects before and after a VLCKD. Twenty obese subjects were enrolled. At baseline and after eight weeks of intervention, anthropometric and biochemical parameters, such as adiponectin levels, were recorded. Our findings showed a significant change in the anthropometric and biochemical parameters of these obese subjects before and after a VLCKD. We found a negative correlation between adiponectin and lipid profile, visceral adipose tissue (VAT), C-reactive protein (CRP), and pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), which confirmed the important involvement of adiponectin in metabolic and inflammatory diseases. We demonstrated the beneficial short-term effects of a VLCKD not only in the treatment of obesity but also in the establishment of obesity-correlated diseases.
A PIANO (Proper, Insufficient, Aberrant, and NO Reprogramming) Response to the Yamanaka Factors in the Initial Stages of Human iPSC Reprogramming
Authors: Hu Kejin
Published: May 2020

Yamanaka reprogramming is revolutionary but inefficient, slow, and stochastic. The underlying molecular events for these mixed outcomes of induction of pluripotent stem cells (iPSC) reprogramming is still unclear. Previous studies about transcriptional responses to reprogramming overlooked human reprogramming and are compromised by the fact that only a rare population proceeds towards pluripotency, and a significant amount of the collected transcriptional data may not represent the positive reprogramming. We recently developed a concept of reprogramome, which allows one to study the early transcriptional responses to the Yamanaka factors in the perspective of reprogramming legitimacy of a gene response to reprogramming. Using RNA-seq, this study scored 579 genes successfully reprogrammed within 48 h, indicating the potency of the reprogramming factors. This report also tallied 438 genes reprogrammed significantly but insufficiently up to 72 h, indicating a positive drive with some inadequacy of the Yamanaka factors. In addition, 953 member genes within the reprogramome were transcriptionally irresponsive to reprogramming, showing the inability of the reprogramming factors to directly act on these genes. Furthermore, there were 305 genes undergoing six types of aberrant reprogramming: over, wrong, and unwanted upreprogramming or downreprogramming, revealing significant negative impacts of the Yamanaka factors. The mixed findings about the initial transcriptional responses to the reprogramming factors shed new insights into the robustness as well as limitations of the Yamanaka factors.
Targeting of Phospholipase D1 Ameliorates Collagen-Induced Arthritis via Modulation of Treg and Th17 Cell Imbalance and Suppression of Osteoclastogenesis
Authors: Yoo Hyun Jung , Hwang Won Chan , Min Do Sik
Published: May 2020

Phospholipase D1 (PLD1) plays a crucial role in various inflammatory and autoimmune diseases. Rheumatoid arthritis (RA) is a chronic and systemic autoimmune disease. However, the role of PLD1 in the pathogenesis of RA remains unknown. Here, we first investigated the role and effects of PLD1 in collagen-induced arthritis (CIA) and found that genetic and pharmacological inhibition of PLD1 in DBA1/J mice with CIA reduced the incidence of CIA, decreased the clinical score, and abrogated disease symptoms including infiltration of leukocytes, synovial inflammation, bone erosion, and cartilage destruction. Moreover, ablation and inhibition of PLD1 suppressed the production of type II collagen-specific IgG2a autoantibody and proinflammatory cytokines, accompanied by an increase in the regulatory T (Treg) cell population and a decrease in the Th17 cell population in CIA mice. The PLD1 inhibitor also promoted differentiation of Treg cells and suppressed differentiation of Th17 cells in vitro. Furthermore, the PLD1 inhibitor attenuated pathologic bone destruction in CIA mice by suppressing osteoclastogenesis and bone resorption. Thus, our findings indicate that the targeting of PLD1 can ameliorate CIA by modulating the imbalance of Treg and Th17 cells and suppressing osteoclastogenesis, which might be a novel strategy to treat autoimmune diseases, such as RA.
The Interaction of lncRNA XLOC-2222497, AKR1C1, and Progesterone in Porcine Endometrium and Pregnancy
Authors: Su Tao , Yu Haile , Luo Gan , Wang Mengxia , Zhou Changfan , Zhang Long , Hou Bin , Zhang Chi , Liu Min , Xu Dequan
Published: May 2020

The endometrium is an important tissue for pregnancy and plays an important role in reproduction. In this study, high-throughput transcriptome sequencing was performed in endometrium samples of Meishan and Yorkshire pigs on days 18 and 32 of pregnancy. Aldo-keto reductase family 1 member C1 (AKR1C1) was found to be a differentially expressed gene, and was identified by quantitative real-time PCR (qRT-PCR) and Western blot. Immunohistochemistry results revealed the cellular localization of the AKR1C1 protein in the endometrium. Luciferase activity assay demonstrated that the AKR1C1 core promoter region was located in the region from −706 to −564, containing two nuclear factor erythroid 2-related factor 2 (NRF2) binding sites (antioxidant response elements, AREs). XLOC-2222497 was identified as a nuclear long non-coding RNA (lncRNA) highly expressed in the endometrium. XLOC-2222497 overexpression and knockdown have an effect on the expression of AKR1C1. Endocrinologic measurement showed the difference in progesterone levels between Meishan and Yorkshire pigs. Progesterone treatment upregulated AKR1C1 and XLOC-2222497 expression in porcine endometrial epithelial cells. In conclusion, transcriptome analysis revealed differentially expressed transcripts during the early pregnancy process. Further experiments demonstrated the interaction of XLOC-2222497/AKR1C1/progesterone in the endometrium and provided new potential targets for pregnancy maintenance and its control.