bluuebunny/crossref_metadata_2025 huggingface dataset:
Histopathology and Ultrastructure of Segmented Filamentous Bacteria–Associated Rainbow Trout Gastroenteritis
Authors: Del-Pozo J. , Crumlish M. , Turnbull J. F. , Ferguson H. W.
Published: January 2010

Rainbow trout gastroenteritis (RTGE) is an emerging syndrome linked to the presence of large numbers of the segmented filamentous bacterium “ Candidatus arthromitus” within the intestine. The present study examined the histopathological changes of the digestive tract of 152 trout with gross lesions typical of RTGE. Histopathology showed that 129 of 152 fish (85%) affected with RTGE had segmented filamentous bacteria in the distal intestine and/or pyloric caeca. The presence and number of segmented filamentous bacteria were always significantly higher ( P < .001) in pyloric caeca, thereby suggesting the preferred site for these bacteria. Histopathological changes included enterocyte detachment and congestion of the lamina propria and adventitial layers. Samples from 6 RTGE-affected trout were examined using scanning and transmission electron microscopy, revealing a close interaction of segmented filamentous bacteria with the mucosa of distal intestine and pyloric caeca, with the presence of bacterial attachment sites, and with associated morphological changes of the apical membrane of enterocytes. Despite these interactions, segmented filamentous bacteria were not always adjacent to the areas with pathological changes, suggesting that if these organisms play a role in the pathogenesis of RTGE, extracellular products may be involved. Ultrastructural changes included loss of microvillar structure, membrane blebbing, hydropic mitochondrial damage, and basal hydropic degeneration of enterocytes, which frequently resulted in disruption of tight junctions and enterocyte detachment. The resulting exposure of large areas of lamina propria probably resulted in the compromise of the host osmotic balance and the facilitation of the entry of secondary pathogens.
Enteric Viruses of Nonhuman Primates
Authors: Kalter S. S.
Published: January 2010

The phylogenetic relationship of nonhuman primates to man implies that many of these animals could serve as surrogates for studies of diseases of man. Many nonhuman primate species are susceptible not only to viruses of human origin but also to nonhuman primate viruses that are counterparts of viruses of man. All monkeys and great apes do not respond similarly to an antigenic stimulus. Some agents are highly pathogenic for one species and completely innocuous for another. For example, poliovirus causes disease and fatalities in great apes, but picornaviruses given orally cause few lesions in most nonhuman primates. Other enteroviruses (coxsackie-, echoviruses) have caused disease in nonhuman primates. It is difficult to separate viruses into distinct categories according to their anatomic affinities. Many viruses not considered to be enteric may be recovered from the intestinal tract. Adenoviruses, both human and nonhuman strains, which are not considered enteric viruses, nonetheless are recovered frequently from the intestinal tract. Adult animals show little evidence of disease, with the possible exception of diarrhea, after adenovirus infection. Newborns, however, may respond with a fatal pneumoenteritis. Adenovirus may be associated with diseases in organs other than the intestines. The reoviruses, which may be recovered from the intestinal tract, also are generally innocuous. Rotaviruses as pathogens in nonhuman primates are presently under study, and it is suspected that rotaviruses of man may produce experimental disease in nonhuman primates. Production of diabetes by several of the enteric viruses has been suggested but not demonstrated conclusively.
The Pathoparasitology of the Alimentary Tract and Pancreas of Nonhuman Primates: A Review
Authors: Toft J. D.
Published: January 2010

A review of the literature concerning the gross and histologic lesions associated with protozoal and metazoal parasitism in the alimentary tract and pancreas of nonhuman primates is presented. In addition, the natural history, morphology, life cycle, methods for diagnosis, and potential for zoonotic disease are reviewed briefly for each parasite discussed. The parasite species reviewed in detail are those most common or most likely to produce lesions in the alimentary tract and pancreas of the nonhuman primate host. All parasites, both pathogenic and nonpathogenic, in each major group (protozoa: flagellates, sarcodines, sporozoans, neosporans, and ciliates; and metazoa: trematodes, cestodes, nematodes, acanthocephalans, and pentastomids) that have been reported in the nonhuman primate alimentary tract and pancreas are presented in tables.
Mycotic Infections of the Alimentary Tract of Nonhuman Primates: A Review
Authors: Migaki G. , Schmidt R. E. , Toft J. D. , Kaufmann A. F.
Published: January 2010

Lesions of candidiasis, mucormycosis (phycomycosis), entomophthoramycosis, geotrichosis, cryptococcosis, paracoccidioidomycosis and coccidioidomycosis have been reported in the alimentary tract of nonhuman primates. Candidiasis and mucormycosis were reported most often. Both Old and New World monkeys and great apes are susceptible; infection is rare in prosimians. Ulcers and necrosis of the mucosa of the alimentary tract are the principal gross lesions. A granulomatous inflammatory process occurs in which the fungi are visible histologically on hematoxylin and eosin (HE)-stained sections, but they are seen and characterized better when stained with periodic acid-Schiff (PAS) or Gomori methenamine silver (GMS) techniques. Cultural or immunofluorescence studies, or both, are necessary for specific identification of the fungi. Immunosuppression is suggested as a predisposing factor in certain mycotic diseases.
Acute Gastric Dilatation in Nonhuman Primates: Review and Case Studies
Authors: Pond C. L. , Newcomer C. E. , Anver M. R.
Published: January 2010

Acute gastric dilatation occurs sporadically in laboratory-housed nonhuman primates. Clinical histories often include chronic drug administration, food restriction, accidental overfeeding, and prior anesthesia. Monkeys may be found dead or may have clinical signs of colic, abdominal distention, and dyspnea. Death in untreated cases is due to impaired venous return and cardiopulmonary failure. Gastric distention with fermented gaseous ingesta and congestion of the abdominal viscera are the predominant lesions. The cause of acute gastric dilatation is unknown, but it probably is multifactorial. Two principal factors seem to be intragastric fermentation associated with Clostridium perfringens, and abnormal gastric function.
Adenoviral Pancreatitis in Rhesus Monkeys: Current Knowledge
Authors: Chandler F. W. , McClure H. M.
Published: January 2010

Features and pathogenesis of adenoviral pancreatitis in rhesus monkeys were studied with an immunofluorescence staining procedure on tissues from two previously documented cases. Fluorescing adenovirus antigen in epithelial cells of the pancreatic ducts, duodenum, and jejunum suggests that under as yet undefined conditions, a primary adenovirus infection of the gastrointestinal tract ascends to the pancreatic parenchyma via pancreatic ducts.

In a retrospective survey, over 3,000 microslides of pancreas taken at necropsy from several species of nonhuman primates (1,002 animals) were studied to determine the incidence of and species susceptibility to adenoviral pancreatitis. Other than the two documented cases from our files, we found comparable lesions in only one rhesus monkey. Adenoviral pancreatitis seems to be a distinct entity in rhesus monkeys, and it should be considered when pancreatitis is found in this species. Our findings also suggest a possible viral cause for some cases of pancreatitis in man.
Insular Amyloidosis in Spontaneously Diabetic Nonhuman Primates
Authors: Palotay J. L. , Howard C. F.
Published: January 2010

Sections of pancreas from 21 nonhuman primates with diabetes mellitus were examined by light and electron microscopy. All monkeys showed amyloid accumulation in the islets of Langerhans. Amyloid was identified by its dichroism with three different stains: Congo red, changing from red to yellowish-green; standardized toluidine blue, changing from blue to red; and sulfated alcian blue, changing from blue-green to pink. Sulfated alcian blue was a rapid and effective means of detecting amyloid. The characteristic fibrillar structure of amyloid was seen with transmission electron microscopy. Deposition of islet amyloid was independent of the presence or absence of amyloid in other organs. Results indicate that nonhuman primates offer a model for studying the sequential development of insular amyloidotic diabetes mellitus.
A Scanning Electron Microscopic Study on Surface Lesions in Fluorosed Enamel of Roe Deer (Capreolus capreolusL.)
Authors: Kierdorf U. , Kierdorf H.
Published: January 2010

Scanning electron microscopy of surface enamel lesions in fluorosed permanent premolars and molars of free-ranging Roe deer revealed two types of pits. Post-eruptive lesions that resulted from mechanical stress on hypomineralized enamel during mastication were characterized by steep walls and a typical honeycomb structure on their bottom, a result of fracture of enamel rods; holes left by fractured rods were surrounded by interred enamel. Pits of developmental origin (hypoplasias), either as shallow depressions of enamel surfaces or narrow holes running deep into the enamel, were characterized by convex, rounded walls covered by numerous Tomes' process pits. Pits of hypoplastic and post-eruptive origin were often found in the same tooth. Intact enamel surfaces of fluorosed teeth resembled that of controls.
Conjunctiva-associated Lymphoid Tissue (CALT) in Normal andBordetella avium-infected Turkeys
Authors: Fix A. S. , Arp L. H.
Published: January 2010

Conjunctiva-associated lymphoid tissue (CALT) was characterized in normal and Bordetella avium-infected turkey poults during the first 5 weeks of life. At 1, 5, 12, 19, 25, and 33 days post-hatching (DPH), upper and lower eyelids were examined by gross, histologic, and electron microscopic techniques. CALT was confined to the proximal part of the lower eyelid near the conjunctival fornix; it appeared by 5 DPH as individual lymphoid nodules and as dense masses by 19 DPH. In the upper eyelid, CALT was present only as isolated nodules. Histologically. CALT was composed of dense lymphocyte infiltrates within subepithelial connective tissue, intraepithelial lymphocytes, and flattened lymphoid-associated epithelium that lacked goblet cells. Germinal centers were in CALT by 19 DPH. By scanning electron microscopy, epithelial cells over lymphoid areas were flat and had short, irregular microvilli; non-lymphoid areas were covered by cells with tall, regular microvilli. Transmission electron microscopy revealed that with increasing age of birds, the epithelium over conjunctival lymphoid infiltrates became progressively flattened and infiltrated by lymphocytes. Some blood vessels in CALT had high endothelial cells; lymphocytes were in the lumen and between or beneath endothelial cells. In B. avium- infected poults. CALT was increased, developed earlier, and contained more germinal centers than in normal poults. We conclude that CALT of turkeys closely resembles other mucosal lymphoid tissues and may serve as a site for local antigen uptake.
Evidence that Blood-borne Infection is Involved in the Pathogenesis of Bovine Pneumonic Pasteurellosis
Authors: Thomas L. H. , Gourlay R. N. , Wyld S. G. , Parsons K. R. , Chanter N.
Published: January 2010

Five calves were inoculated intravenously with 108colony forming units (cfu) of Pasteurella haemolytica Al; the mean score for pneumonic consolidation 3 days post-inoculation was 28%, and the mean clinical score was 7.8. Five calves inoculated intratracheally with 109cfu of the same strain of P. haemolytica had comparable scores (34% and 8.8). Histological lesions of fibrinous pneumonia were similar in all calves. P. haemolytica was recovered from all but one of the affected lungs. From one calf killed in extremis 3 hours after intravenous inoculation, numbers of bacteria recovered from lung were 1,000-fold greater than from liver and spleen. A similar difference in bacterial numbers was also obtained from a gnotobiotic calf killed in extremis, 12 hours after intravenous inoculation of 108cfu P. haemolytica. Evidence from these experiments supports the hypothesis that the blood-borne route is important in the pathogenesis of bovine pneumonic pasteurellosis.
Bronchoalveolar Lavage Cytology in Cynomolgus Monkeys and Identification of Cytologic Alterations Following Sequential Saline Lavage
Authors: Haley P. J. , Muggenburg B. A. , Rebar A. H. , Shopp G. M. , Bice D. E.
Published: January 2010

Total and differential cell counts were determined on cytolytic specimens obtained by fiberoptic bronchoscopy and bronchoalveolar lavage (BAL) of five normal cynomolgus monkeys. Total nucleated cell counts ranged from 100 to 430 cells/μl. Macrophages were approximately 91% of total nucleated cells, while lymphocytes were 3%, neutrophils 4%, and eosinophils 2% of the initial BAL from each monkey. Less than 1% of the cells were mast cells and ciliated or nonciliated epithelial cells. The effects of repeated saline BAL on pulmonary cell populations were evaluated. Saline lavage of individual lung lobes resulted in a marked rise in circulating blood neutrophils at 4 hr after BAL; there was a similar rise in neutrophils in lavage fluids 24 hr after the initial lavage. Differential and total cell counts of both blood and lavage fluid returned to normal if subsequent lavages were spaced at 48-hr intervals. Lymphocytes were not present in saline-lavaged lung lobes, and protein levels of lavage fluids did not rise significantly. BAL produced a transient, reversible, intra-alveolar influx of neutrophils which was preceded by mobilization of bone marrow-stored neutrophils. Neutrophilia in the lavage fluid and blood was not detectable if lavage and blood sampling procedures were done at 48-hr intervals (which did not alter Ia antigen expression among BAL cells). These observations indicate that BAL is a valid method for sampling and assessing pulmonary cellular and fluid constituents if the procedures are done at intervals of at least 48 hr.
Spontaneous Equine Pulmonary Granular Cell Tumors: Morphologic, Histochemical, and Immunohistochemical Characterization
Authors: Kelley L. C. , Hill J. E. , Hafner S. , Wortham K. J.
Published: January 2010

Spontaneous equine pulmonary granular cell tumors were diagnosed in six mature horses at slaughter. These tumors were grossly recognized as multiple (5/6) or single (1/6) creamy white, firm nodules. The tumors, located adjacent to bronchi and bronchioles, often invaded airways, resulting in partial to complete occlusion of the lumina. Neoplastic cells were rounded to polyhedral with numerous eosinophilic cytoplasmic granules that reacted uniformly positive with S-100 and neuron-specific enolase antibodies and multifocally with glial fibrillary acidic protein antibodies. These cells were negative for muscle-specific actin, lysozyme, cytokeratin, chromogranin A, and myelin basic protein antigens and did not stain with silver by the Grimelius technique. Uniformly blue-green and scattered pink intracytoplasmic granules were evident with luxol fast blue and periodic acid-Schiff counterstain for myelin and myelin breakdown products. Histochemical and immunohistochemical staining results of these tumors suggest that they are composed primarily of myelinating Schwann cells with lesser numbers of scattered nonmyelinating Schwann cells. The morphologic features of the equine pulmonary granular cell tumors are strikingly similar to those of endobronchial granular cell tumors of human beings.
Demonstration ofMycoplasma hyorhinisas a Possible Primary Pathogen for Porcine Otitis Media
Authors: Morita T. , Fukuda H. , Awakura T. , Shimada A. , Umemura T. , Kazama S. , Yagihashi T.
Published: January 2010

A study of the pathology of the ear was performed on 479 pigs ranging in age from 1 day to 1 year. Histologically, 364 (76.0%) of 479 pigs were affected with otitis. Eustachitis was the most common and preceded an inflammation of other sites of the ear, and an acute eustachitis occurred from as early as 1 week of life. Immunohistochemical examination of frozen cryostat sections revealed Mycoplasma hyorhinis (Mhr) antigens on the luminal surface of the eustachian epithelia in 14 (50.0%) of 28 piglets examined. All the pigs positive for Mhr had an acute eustachitis. Ultrastructural examination on the auditory tubes with positive immunostaining disclosed many mycoplasmas among the cilia. Mhr was isolated from the auditory tubes and tympanic cavities of 19 (67.9%) and 16 (57.1%) of 28 piglets examined, respectively. Porcine otitis media may be caused primarily by Mhr infection in the auditory tube.
Feline Endomyocarditis and Left Ventricular Endocardial Fibrosis
Authors: Stalis I. H. , Bossbaly M. J. , Van Winkle T. J.
Published: January 2010

A retrospective study was conducted of all feline necropsies over a 7-year period. Of a total of 1,472 necropsies, 37 cases of endomyocarditis (EMC) and 25 cases of left ventricular endocardial fibrosis (LVEF) (previously called restrictive or intermediate cardiomyopathy) were identified. There was a subset of four cats with EMC that had histologic features of both diseases. Interstitial pneumonia was seen in 25 of 35 cats (71%) with EMC but in only seven of 25 cats (28%) with LVEF. Thrombi or thromboemboli were seen in 14 of 25 cats (56%) with LVEF but in only six of 37 of cats (16%) with EMC. In both LVEF and EMC, thromboemboli were located in the abdominal aorta, left atrium and ventricle of the heart, femoral artery, cranial mesenteric artery, liver, pulmonary artery, jugular vein, or a meningeal vessel. Each cat had a single thrombus/thromboembolus, except for four cats with LVEF that had more than one. The histologic and clinical findings suggest that EMC and LVEF represent temporally different manifestations of a single disease entity.
Higher Incidence of Gastrospirillum sp. in Swine with Gastric Ulcer of the Pars oesophagea
Authors: Barbosa A. J. A. , Silva J. C. P. , Nogueira A. M. M. F. , Paulino E. , Miranda C. R.
Published: January 2010

Gastric ulcer in swine is characterized by an area of acid-peptic digestion, occurs usually in the pars oesophagea of the stomach, and has unknown etiopathogenesis. The present work was carried out to investigate the prevalence of the newly described spiral-shaped microorganism Gastrospirillum sp. (“ Gastrospirillum suis”) in stomachs of abattoir pigs with and without gastric ulcer. Stomachs were removed from 32 consecutive pigs presenting apparently normal mucosa and from 32 additional consecutive pigs presenting frank, chronic gastric ulcer of the pars oesophagea. Fragments of antral, oxyntic, cardiac and pars oesophagea regions were taken from each stomach and processed for histology and for identification of Gastrospirillum sp. in tissue sections. The microorganisms were identified mainly in the mucous layer and in gastric foveolas of the antral and oxyntic mucosa. Forty pigs (62.5%) were positive for Gastrospirillum sp.; among them, 27 (67.5%) had gastric ulcer, and 13 (32.5%) had no ulcer. Twenty-four pigs (37.5%) were negative for Gastrospirillum sp.; among them, five (20.8%) presented with gastric ulcer, and 19 (79.2%) had no ulcer. There was a significant difference between pigs with and without gastric ulcer in regard to the presence of Gastrospirillum sp. ( P < 0.01). The spiral-shaped microorganism Gastrospirillum sp. that inhabits the stomach of pigs should be considered a possible factor connected with the etiopathogenesis of swine gastric ulcer.
Immunoreactivity of Canine Transitional Cell Carcinoma of the Urinary Bladder with Monoclonal Antibodies to Tumor-associated Glycoprotein 72
Authors: Clemo F. A. S. , DeNicola D. B. , Carlton W. W. , Walker E. , Morrison W. B.
Published: January 2010

Tumor-associated glycoprotein 72 (TAG-72) is a large, high molecular weight, mucinlike antigen that is expressed in a wide variety of human carcinomas. Three different TAG-72 monoclonal antibodies (MAbs), designated B72.3, CC49, and CC83, were applied to the following archived samples from the dog: 1) 51 transitional cell carcinomas of the urinary bladder, 2) 15 hyperplastic/inflamed urinary bladders, and 3) eight normal urinary bladders. Immunoreactivity was detected with an avidin-biotin complex immunoperoxidase method. Fifty-three percent (27/51) of transitional cell carcinomas were positive (≥ 5% staining) for MAb B72.3. MAb B72.3 staining of these transitional cell carcinomas did not statistically correlate with any of the examined features of malignancy, including histologic grade, clinical stage, DNA ploidy, or presence of vascular/lymphatic invasion. In regard to the staining of transitional cell carcinoma by the other two TAG-72 antibodies, 53% (27/ 51) of the samples were positive for MAb CC83 and 63% (32/51) were positive for MAb CC49. The finding that similar populations of neoplastic urothelial cells in serial sections from the same neoplasm stained with all three TAG-72 antibodies supports the hypothesis that an antigen similar to TAG-72 was expressed in canine transitional cell carcinoma. None of the normal urinary bladders nor any of the hyperplastic/inflamed urinary bladders stained with any of the three TAG-72 antibodies tested. The results of these studies demonstrated that the staining of canine transitional cell carcinoma with all three TAG-72 antibodies was specific for neoplastic urothelial cells and that an antigen similar to TAG-72 was expressed.
In situ Hybridization and Immunohistochemical Study of Walleye Dermal Sarcoma Virus (WDSV) Nucleic Acids and Proteins in Spontaneous Sarcomas of Adult Walleyes (Stizostedion vitreum)
Authors: Poulet F. M. , Vogt V. M. , Bowser P. R. , Casey J. W.
Published: January 2010

Twenty-two anatomically independent dermal sarcomas from six adult walleye fish ( Stizostedion vitreum) collected during the spring from Oneida Lake, New York, were examined by in situ hybridization and immunohistochemistry for the presence of walleye dermal sarcoma virus (WDSV). The viral RNA, DNA, and 90-kd protein were localized at the cellular level. Riboprobes complementary to the 5′ terminal region of WDSV genome were used to detect viral nucleic acids. Rabbit polyclonal antiserum was generated against the 90-kd virus-associated antigen, presumably a product of the env gene, for immunohistochemical studies. Viral transcripts were detected in the neoplastic cells of all dermal sarcomas, in which they were generally abundant. Rare mononuclear inflammatory cells and cells within the epidermis also expressed viral RNA. In all sarcomas, low to moderate levels of viral DNA were present in all neoplastic and most mononuclear inflammatory and epidermal cells. Many neoplastic cells were immunopositive for the virus-associated protein. The distribution of immunopositive neoplastic cells mimicked approximately that of cells containing viral transcripts. The number of neoplastic cells with transcripts exceeded that of cells with protein, suggesting that productively infected neoplastic cells constituted a subset of the neoplastic cells that expressed WDSV transcripts. The viral antigen was also present within many mononuclear inflammatory cells. These data suggested that 1) dermal sarcomas were associated with elevated transcriptional activity of WDSV in the neoplastic cells and 2) the cell tropism of WDSV extended beyond the mesenchymal fibroblast-like neoplastic cells and included at least mononuclear inflammatory and epidermal cells.
Pasteurella haemolyticaLipopolysaccharide-induced Cytotoxicity in Bovine Pulmonary Artery Endothelial Monolayers: Inhibition by Indomethacin
Authors: Paulsen D. B. , Confer A. W. , Clinkenbeard K. D. , Mosier D. A.
Published: January 2010

Exposure of bovine pulmonary artery endothelial cells to Pasteurella haemolytica lipopolysaccharide caused severe morphologic changes. Initially, there was dilatation of the rough endoplasmic reticulum and mitochondrial swelling followed by cell retraction, membrane bleb formation, and cell detachment. The affected endothelial cells had severe membrane damage resulting in the leakage of lactate dehydrogenase. Indomethacin in concentrations of 0.5 mM or greater caused marked decreases in the lipopolysaccharide-induced leakage of lactate dehydrogenase. Indomethacin at 5 mM also caused a marked reduction of the lipopolysaccharide-induced morphologic changes resulting in apparent maintenance of the monolayer integrity for 8 hours versus 1 hour in the lipopolysaccharide-treated control. A marked decrease in the cell and nuclear membrane effects resulted, but the rough endoplasmic reticulum dilatation and mitochondrial changes proceeded. These results indicate that indomethacin does not prevent lipopolysaccharide binding but interferes with later events in lipopolysaccharide-induced cytotoxicity in the bovine pulmonary endothelial cell. The concentration of indomethacin required to produce this inhibition suggests that the primary mechanism is not cyclooxygenase inhibition.
Giant Cell Glioblastoma in the Frontal Cortex of a Dog
Authors: Uchida K. , Kuroki K. , Priosoeryanto B. P. , Kato K. , Yano Y. , Murakami T. , Yamaguchi R. , Tateyama S.
Published: January 2010

A dark gray mass 3 cm in diameter replacing the right frontal cortex was found in the brain of a 5-year-old male Doberman Pinscher dog at necropsy. Microscopic studies revealed that the mass consisted of a proliferation of pleomorphic tumor cells: large bizarre or plump eosinophilic cells, multinucleated giant cells, and small lymphocytic cells. These neoplastic cells at the margin of the necrotic area had a psuedopalisade arrangement and tended to proliferate around blood vessels. Immunohistochemically, the tumor cells reacted intensely with the antibody for vimentin and moderately with those for S-100 and glial fibrillary acidic protein. This canine tumor is placed in the category of glioblastoma or undifferentiated astrocytoma, which is analogous to human giant cell glioblastoma.
A Sequential Light Microscopic and Ultrastructural Study on the Uptake and Handling of Vibrio salmonicida in Phagocytes of the Head Kidney in Experimentally Infected Atlantic Salmon (Salmo salar L.)
Authors: Brattgjerd S. , Evensen Ø.
Published: January 2010

The uptake and handling of Vibrio salmonicida in phagocytes of the head kidney of Atlantic salmon (Salmo salar L.) were evaluated by light and electron microscopy, including in situ identification of the bacterium by immunolabeling at the light microscopical and the ultrastructural level. Fish were injected with live bacteria, and 4, 24, 48, and 72 hours after inoculation, samples were collected after perfusion fixation. Morphologically, the most prominent change in the course of the experiment was an increasing number of intrasinusoidal, endothelial cell-adherent phagocytes and the elevated number of interstitial melanomacrophages. Immunohistochemically, bacterial antigens were initially identified in intrasinusoidal phagocytes, and at 24 hours postinfection in endothelium-adherent phagocytes and intrasinusoidal melanomacrophages. Later, (48 and 72 hours postinfection), the interstitial melanomacrophages were also found to harbor bacterial antigen. Ultrastructurally, bacteria were identified in phagosomes in intrasinusoidal phagocytes, and morphological findings also indicated an increased cellular degradation, including autophagocytosis. Immunoelectron microscopy indicated that bacterial antigens were associated with melanomacrophages, specifically in their electron-dense cytoplasmic granules. These findings indicate that intrasinusoidal phagocytes and melanomacrophages participate in the rapid and active clearance of particulate material from the circulation, i.e., pathogenic microorganisms, and in the scavenging of cellular degradation products. The process of formation of melanomacrophages and their possible function is discussed.
Detection ofMycoplasma ovipneumoniaeandPasteurella haemolyticaAntigens by an Immunoperoxidase Technique in Pneumonic Ovine Lungs
Authors: Haziroglu R. , Diker K. S. , Turkarslan J. , Gulbahar M. Y.
Published: January 2010

Four hundred twenty pneumonic lungs from lambs were examined for Mycoplasma ovipneumoniae and Pasteurella haemolytica by an immunoperoxidase technique using an extravidin-biotin-peroxidase complex method in formalin-fixed, paraffin-embedded sections. Histologic examination of tissue sections revealed strong positive reactions in 60.9% and 68.3% of the lungs against M. ovipneumoniae and P. haemolytica, respectively. M. ovipneumoniae and P. haemolytica antigens were observed at the surface and/or within the epithelial cells, macrophages, leucocytes, and bronchiolar exudate. The location of M. ovipneumoniae in the cytoplasm of the epithelial cells and P. haemolytica in the neutrophils was detected immunohistochemically.
Persistent Oropharyngeal Membrane in a Hereford Calf
Authors: Smoak I. W. , Hudson L. C.
Published: January 2010

Persistent oropharyngeal membrane was found in a 6-day-old Hereford calf. The calf was unable to nurse and had not passed feces since birth. Physical examination revealed a fold of tissue spanning the caudal oral cavity, and a barium study demonstrated that food remained within the oral cavity despite a swallowing reflex. A thickened epiglottis was demonstrated radiographically but was not confirmed on necropsy. Necropsy revealed a complete soft-tissue partition between oral cavity and oropharynx with a central blind-ended diverticulum. Only a few cases of persistent oropharyngeal membrane have been reported previously, all in humans. This is the first report of this defect in domestic animals. Possible similarities to the more common persistent anal membrane are discussed.
Fatal Cytauxzoonosis in a Captive-reared White Tiger (Panthera tigris)
Authors: Garner M. M. , Lung N. P. , Citino S. , Greiner E. C. , Harvey J. W. , Homer B. L.
Published: January 2010

Fatal (Panthera tigris) cytauxzoonosis was diagnosed in a 7-year-old female white tiger. The tiger presented with a 2-day history of anorexia and lethargy. She was mildly dehydrated, with a temperature of 105.2 F and a hematocrit of 26%. Over the next day, icterus developed, and her physical condition progressed to recumbency, coma, and death. Hematologic findings obtained shortly before death included icteric plasma, severe thrombocytopenia, mild anemia, hematuria, and parasites consistent with Cytauxzoon felis in circulating erythrocytes. Gross necropsy findings included generalized icterus, generalized petechiae and ecchymoses, splenomegaly, and peribronchial edema. Histologic changes included large numbers of intravascular macrophages containing developmental stages of Cytauxzoon felis that partially or completely occluded blood vessels in the lung, spleen, liver, and bone marrow. Except for an experimental infection of a bobcat, fatal cytauxzoonosis has not previously been diagnosed in felids other than domestic cats. These findings raise questions regarding the pathogenicity of this organism in felids and may impact husbandry and interstate transfer of captive large cats.
Fetal Microscopic Lesions in Porcine Reproductive and Respiratory Syndrome Virus-induced Abortion
Authors: Rossow K. D. , Laube K. L. , Goyal S. M. , Collins J. E.
Published: January 2010

Microscopic lesions in four cases of abortion caused by porcine reproductive and respiratory syndrome virus (PRRSV) were characterized by arteritis, myocarditis, and encephalitis. Fetal PRRSV infection was confirmed by virus isolation or immunohistochemistry and serology. Although fetal microscopic lesions in PRRSV abortions are rarely reported, diagnosticians should consider PRRSV when these lesions occur.
Social Security and Its Impact on Older Latinos
Authors: Kim Bum Jung , Torres-Gil Fernando
Published: January 2010

Older Latinos have distinct demographic and economic characteristics that are making their reliance on Social Security critical. Latinos are the fastest growing segment of the older adult population. They have lower than average educational levels and higher rates of poverty than other subgroups. In addition, they have small accumulated wealth and limited retirement income. Social Security is a particularly important source of income for older Latinos because it provides many older Latinos with their sole or primary sources of income in retirement. Without Social Security, more than half of older Latinos would live in poverty. This study finds four factors that make Social Security beneficial to older Latinos: (a) lower lifetime incomes, (b) longer life expectancies, (c) higher disability incidence, and (d) higher marriage rates. Because of these factors, older Latinos receive a higher average rate of return on their Social Security contributions. The study also provides policy implications for economic security of older Latinos.
Political Homophobia in Postcolonial Namibia
Authors: Currier Ashley
Published: January 2010

The South West African People’s Organisation (SWAPO) delivered Namibia from South African apartheid rule in 1990. Namibia’s democratic future began with the promise of equality. In 1995, however, SWAPO initiated a campaign of political homophobia. In this article, I make a case for viewing SWAPO leaders’ deployment of political homophobia as a gendered political strategy. I draw on a qualitative analysis of 194 articles from Namibian newspapers published between 1995 and 2006. My analysis illustrates two features of political homophobia. First, I demonstrate how political homophobia stifled political dissent and enhanced SWAPO leaders’ masculinist position and legacy as liberators. Second, I show how SWAPO leaders used political homophobia to expel gender and sexual dissidents from official accounts of history.
Love Matches
Authors: Esacove Anne W.
Published: January 2010

This article identifies the dominant public narrative of AIDS in Malawi through an analysis of qualitative interview data and policy and intervention materials. The public narrative creates distinctions between “risky” and “healthy” sex that organize HIV prevention efforts around moral categories, rather than relative risk. These distinctions oppose images of backward, ignorant villagers to the protective power of “love matches” (loving heterosexual relationships between equals). The analysis demonstrates that the public narrative and corresponding prevention efforts only make sense in connection with the patently false assumption that love, heterosexuality, and modernity effectively protect individuals from HIV. This research brings to light the unspoken assumptions of modernity in the (Western) “charmed circle” of heteronormativity, as well as the need to consider the workings of heteronormativity in studies of modernization and globalization. Furthermore, it highlights individualistic and oversimplified analytic models currently directing HIV prevention efforts and points to potentially more effective strategies.
Men Just Weren’t Made To Do This
Authors: Bridges Tristan S.
Published: January 2010

Though there is a vast literature on performances of drag, performances of gender and sexual transgressions outside of drag clubs are less studied. This case study of men’s marches protesting violence against women—“Walk a Mile in Her Shoes” marches— examines the politics of such transgressions. Cross-dressing to various degrees is strategically utilized at these events in an attempt to encourage men to become empathetic allies. This article suggests, however, that context is critical to the political potential of performances of drag. The author’s observations of the interactions at the marches suggest that drag at “Walk a Mile” marches often symbolically reproduces gender and sexual inequality despite good intentions. At these marches, feminism is gendered when performances of politics and protest are contextually framed as gender and/or sexual transgressions when “feminism” is understood as “feminine.”
Acute selenium poisoning by paradise nuts (Lecythis ollaria)
Authors: Müller Dieter , Desel Herbert
Published: January 2010

Two previously healthy women developed nausea, vomiting, headache and dizziness for several days, a massive hair loss about 2 weeks later and a discoloration of the fingernails. Detailed diagnostic procedures did not reveal any pathological results. Therapeutic measures did not show any effect. Thallium and arsenic were within normal range in plasma. Delayed quantitative determination of selenium in blood, however revealed toxic values (in case I: 479 μg/L of serum, 8 weeks after ingestion, and in case II 300 μg/L of serum, 9 weeks after ingestion). In retrospect, a relation to the ingestion of paradise nuts could be established.
Organizational Support, Individual Attributes, and the Practice of Career Self-Management Behavior
Authors: Sturges Jane , Conway Neil , Liefooghe Andreas
Published: January 2010

This article reports the findings of a study, conducted in a UK new media company, that investigated direct and interactive relationships between perceived organizational support (POS), leader—member exchange (LMX), gender, locus of control, and practice of career self-management behaviors. The results show that it is the interactive relationships that have stronger links with internally focused career self-management behavior, whereas the direct associations, with the exception of LMX, are more closely related to externally oriented career self-management activities. POS moderates the relationship between both gender and locus of control and internally focused career self-management behavior, suggesting that it may send out a signal to certain employees about how supportive the organizational environment is of career self-management. Men and women are shown to act in different ways, depending on the level of POS that they receive, whereas POS encourages individuals with an internal locus of control to engage in internal career self-management behavior, in line with trait activation theory. LMX operates more directly, suggesting that it is a source of practical help with career self-management aimed at furthering the career within the organization; interaction results show that, when LMX is absent, men and those with an internal locus of control engage in internal networking behavior, presumably to find other sources of such help.
Continuous long-term immunomodulatory therapy in relapsing multiple sclerosis: results from the 15-year analysis of the US prospective open-label study of glatiramer acetate
Authors: Ford C. , Goodman AD , Johnson K. , Kachuck N. , Lindsey JW , Lisak R. , Luzzio C. , Myers L. , Panitch H. , Preiningerova J. , Pruitt A. , Rose J. , Rus H. , Wolinsky J.
Published: January 2010

The ongoing US Glatiramer Acetate (GA) Trial is the longest evaluation of continuous immunomodulatory therapy in relapsing—remitting multiple sclerosis (RRMS). The objective of this study was to evaluate up to 15 years of GA as a sole disease-modifying therapy. Two hundred and thirty-two patients received at least one GA dose since study initiation in 1991 (mITT cohort), and 100 (43%, Ongoing cohort) continued as of February 2008. Patients were evaluated every 6 months using the Expanded Disability Status Scale (EDSS). Mean GA exposures were 8.6 ± 5.2, 4.81 ± 3.69, and 13.6 ± 1.3 years and mean disease durations were 17, 13, and 22 years for mITT, Withdrawn and Ongoing cohorts, respectively. For Ongoing patients, annual relapse rates (ARRs) maintained a decline from 1.12 ± 0.82 at baseline to 0.25 ± 0.34 per year; 57% had stable/improved EDSS scores (change ≤ 0.5 points); 65% had not transitioned to secondary progressive multiple sclerosis (SPMS); 38%, 18%, and 3% reached EDSS 4, 6, and 8. For all patients on GA therapy (the mITT cohort), ARRs declined from 1.18 ± 0.82 to 0.43 ± 0.58 per year; 54% had stable/improved EDSS scores; 75% had not transitioned to SPMS; 39%, 23%, and 5% reached EDSS 4, 6, and 8. In conclusion, multiple sclerosis patients with mean disease duration of 22 years administering GA for up to 15 years had reduced relapse rates, and decreased disability progression and transition to SPMS. There were no long-term safety issues.
The Plight of Caring for Young Patients With Frontotemporal Dementia
Authors: Chemali Z. , Withall A. , Daffner K.R.
Published: January 2010

We present the case of a 39-year-old patient with frontotemporal dementia. This case depicts the complexities in the process leading to the diagnosis, treatment, and placement of young patients presenting with severe psychiatric symptoms as the first signs of an underlying neurological disease. Obstacles in the health care system and residential placement process that hinder the optimal and timely care of such difficult cases are discussed. Practical solutions are offered that center upon better awareness and education and the provision of additional resources. These interventions are likely to provide a positive return on investment for the medical system and could be used as strong levers for new health policies relevant to younger patients with neurological illness.
Pacing and Lapping Movements Among Institutionalized Patients With Dementia
Authors: Nakaoka Akiko , Suto Shunji , Makimoto Kiyoko , Yamakawa Miyae , Shigenobu Kazue , Tabushi Kaoru
Published: January 2010

Wandering is a complex behavior, and defining wandering has been challenging. The current study used the integrated circuit (IC) tag monitoring system to describe the distance moved per day and the spatial movements of patients with dementia. The study was conducted in a 60-bed semiacute dementia care unit in a general hospital in Japan over a 3-month period in 2006. The distance moved per day, the numbers of pacing and lapping movements, and the proportions of the distance moved that was paced or lapped were tabulated in 23 patients diagnosed with dementia. The distance moved per day and the numbers of pacing and lapping movements varied greatly within and among study participants. The median distance moved per day was inversely correlated with participants’ age and Mini-Mental State Examination (MMSE) scores (adjusted r2 = .34, P = .01). Consecutive lapping and pacing movements were rare patients with in Alzheimer’s disease (AD), while 2 patients with frontotemporal dementia paced or lapped repeatedly.
Case Scenarios and Simulations as Techniques to Facilitate Asthma Education and Team Building Among Health Care Students
Authors: McCormick Margaret J. , Burton Tamara , Werts Niya
Published: January 2010

Health professionals face increasing demands to work across discipline lines to improve patient outcomes. Learning interdisciplinary communication skills at the preprofessional level is crucial to nursing and allied health student preparation. Today’s students are technologically savvy, tired of traditional education, and often have diverse learning needs. The purpose of the pilot study was to examine the effectiveness of an interdisciplinary case study and simulation model to educate students enrolled in various health professions about asthma care and patient education. The interdisciplinary case study simulation model showed a significant advantage for both student enjoyment of the learning process and students’ confidence to coordinate care across disciplines.
Identification of novel downstream targets of platelet glycoprotein VI activation by differential proteome analysis: implications for thrombus formation
Authors: Schulz Christian , Leuschen Nina V. , Fröhlich Thomas , Lorenz Michael , Pfeiler Susanne , Gleissner Christian A. , Kremmer Elisabeth , Kessler Mirjam , Khandoga Alexander G. , Engelmann Bernd , Ley Klaus , Massberg Steffen , Arnold Georg J.
Published: January 2010

Platelets play a key role in hemostasis and various diseases including arterial thrombosis. Glycoprotein VI (GPVI) mediates adhesion to collagen structures exposed at sites of vascular injury and subsequent platelet activation. We determined the effects of specific activation of GPVI on the human platelet proteome. Isolated human platelets were stimulated with an activating monoclonal antibody specific for GPVI. Platelet proteins were analyzed by 2-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry. We identified 8 differentially abundant proteins associated with cell signaling, metabolism, organization and rearrangement of the cytoskeleton, and membrane trafficking. Differentially abundant proteins included aldose reductase (AR), beta-centractin, charged multivesicular body protein 3, Src substrate cortactin, ERp57, and pleckstrin. Importantly, GPVI-modulated protein abundance was functionally relevant. Correspondingly, AR enzyme activity significantly increased upon GPVI activation and inhibition of AR resulted in reduced platelet aggregation. Furthermore, ERp57 was released upon ligation of platelet GPVI and increased the activity of tissue factor, a major initiator of blood coagulation. In summary, GPVI activation results in differential changes in abundance of platelet proteins, including AR and ERp57, which support platelet aggregation and platelet-dependent coagulation. These results provide further insight into the mechanisms that underlie platelet activation through the GPVI receptor and may help to identify novel pharmacologic targets.
Copy neutral loss of heterozygosity: a novel chromosomal lesion in myeloid malignancies
Authors: O'Keefe Christine , McDevitt Michael A. , Maciejewski Jaroslaw P.
Published: January 2010

Single nucleotide polymorphism arrays (SNP-A) have recently been widely applied as a powerful karyotyping tool in numerous translational cancer studies. SNP-A complements traditional metaphase cytogenetics with the unique ability to delineate a previously hidden chromosomal defect, copy neutral loss of heterozygosity (CN-LOH). Emerging data demonstrate that selected hematologic malignancies exhibit abundant CN-LOH, often in the setting of a normal metaphase karyotype and no previously identified clonal marker. In this review, we explore emerging biologic and clinical features of CN-LOH relevant to hematologic malignancies. In myeloid malignancies, CN-LOH has been associated with the duplication of oncogenic mutations with concomitant loss of the normal allele. Examples include JAK2, MPL, c-KIT, and FLT3. More recent investigations have focused on evaluation of candidate genes contained in common CN-LOH and deletion regions and have led to the discovery of tumor suppressor genes, including c-CBL and family members, as well as TET2. Investigations into the underlying mechanisms generating CN-LOH have great promise for elucidating general cancer mechanisms. We anticipate that further detailed characterization of CN-LOH lesions will probably facilitate our discovery of a more complete set of pathogenic molecular lesions, disease and prognosis markers, and better understanding of the initiation and progression of hematologic malignancies.