bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Data from The Zinc-Finger AN1-Type Domain 2a Gene Acts as a Regulator of Cell Survival in Human Melanoma: Role of E3-Ligase cIAP2
Authors: Pizzato Scomazzon Sofia , Riccio Anna , Santopolo Silvia , Lanzilli Giulia , Coccia Marta , Rossi Antonio , Santoro M. Gabriella
Published: April 2023

Supplementary Table 1. Primers and siRNAs used. Supplementary Table 2. Antibodies used. Supplementary Figure S1. Effect of different proteasome inhibitors on AIRAP expression in M10 melanoma cells. Supplementary Figure S2. Intracellular localization of AIRAP in bortezomib-treated M10 melanoma cells. Supplementary Figure S3. Specificity of AIRAP silencing in human melanoma cells. Supplementary Figure S4. AIRAP-knockdown enhances the anticancer activity of bortezomib long-term treatment in melanoma cells. Supplementary Figure S5. AIRAP-silencing promotes PARP-cleavage and reduces colony-forming ability in different melanoma cell lines. Supplementary Figure S6. AIRAP-silencing inhibits colony-forming ability in A375 melanoma cells. Supplementary Figure S7. AIRAP-knockdown inhibits SK-MEL-28 melanoma spheroid growth.
Figure S1 from YAP Tyrosine Phosphorylation and Nuclear Localization in Cholangiocarcinoma Cells Are Regulated by LCK and Independent of LATS Activity
Authors: Sugihara Takaaki , Werneburg Nathan W. , Hernandez Matthew C. , Yang Lin , Kabashima Ayano , Hirsova Petra , Yohanathan Lavanya , Sosa Carlos , Truty Mark J. , Vasmatzis George , Gores Gregory J. , Smoot Rory L.
Published: April 2023

Characterization by RT-PCR of Src Family Kinase members expressed in three additional unique PDX.
Supplementary Tables 1 - 3 from Integration of Distinct ShcA Signaling Complexes Promotes Breast Tumor Growth and Tyrosine Kinase Inhibitor Resistance
Authors: Ha Jacqueline R. , Ahn Ryuhjin , Smith Harvey W. , Sabourin Valerie , Hébert Steven , Cepeda Cañedo Eduardo , Im Young Kyuen , Kleinman Claudia L. , Muller William J. , Ursini-Siegel Josie
Published: April 2023

Supplementary Table 1: List of differentially expressed genes comprising the ShcA-PTBMUT-like gene signature (GEO GSE41718) used for Figure S7. Supplementary Table 2: Antibodies used for immunoblot analysis (referred to in the supplementary methods). Supplementary Table 3: Antibodies used for immunohistochemistry (referred to in the supplementary methods).
Supplementary Figures 1 - 8 from Nucleoside Diphosphate Kinase-3 (NME3) Enhances TLR5-Induced NFκB Activation
Authors: Flentie Kelly , Gonzalez Caleb , Kocher Brandon , Wang Yue , Zhu Hongtu , Marasa Jayne , Piwnica-Worms David
Published: April 2023

Supplementary Figures 1-8. Figure S1. Dosed-dependent activation of transcriptionally-activated κB5:FLuc reporter by flagellin and heat-killed Salmonella in HCT116 colon carcinoma cells. Figure S2. Inhibition of the transcriptionally-coupled κB5:IκBα-FLuc fusion reporter by shTLR5 is rescued by increasing the flagellin concentration. Figure S3. High-throughput screening data. Normalized photon flux data for 691 targeted kinases is shown at 45 min (a) and 245 min (b) after Salmonella stimulation. Figure S4. NME3 regulates TLR5-induced NF-κB signaling. Figure 5S. TLR5 mRNA levels correlate with overall patient survival in various cancers. Figure S6. NF-κB1 mRNA levels significantly correlate with enhanced patient survival in breast cancer. Gehan-Breslow-Wilcoxon test were used to estimate the p-values between high and low expression groups (using top and bottom 25% as the cutoff for grouping). Figure S7. CDK6 mRNA levels correlate with overall poorer patient survival in various cancers. Gehan-Breslow-Wilcoxon test were used to estimate the p-values between high and low expression groups (using top and bottom 25% as the cutoff for grouping). Figure S8. NME3 exhibits mutations within or adjacent to the kinase-active site in various cancers.
Figure S4 from Novel Regulation of Integrin Trafficking by Rab11-FIP5 in Aggressive Prostate Cancer
Authors: Das Lipsa , Gard Jaime M.C. , Prekeris Rytis , Nagle Raymond B. , Morrissey Colm , Knudsen Beatrice S. , Miranti Cindy K. , Cress Anne E.
Published: April 2023

Supplementary Figure 4. α3 integrin recycled to lamellipodia. Surface α3 integrin was labelled with P1B5 rabbit antibody in adherent DU145 cell monolayer and allowed to internalize for 40 minutes at 37{degree sign}C. Uninternalized label remaining at the cell membrane is detected and blocked from internalization using Alexa 568 conjugated anti-rabbit secondary antibody. P1B5 antibody bound integrin protected inside the cells was allowed to recycle back and subsequently reacted with Alexa 488 conjugated anti-rabbit secondary antibody. (A) Total membrane and internalized intracellular α3 integrin in permeabilized cells shown as control (red). (B) Uninternalized α3 integrin (red) and recycled α3 integrin (green) at 0min, 10min and 40 minutes of recycling and 40min recycling with primaquine (PQ, recycling inhibitor) are shown in merged images with DAPI (blue) stained nucleus (left panel). Middle panel shows only the recycled α3 integrin label in grey. Right panel is magnified images of boxed sections marked for recycled integrin localized at lamellipodia at cell front (white arrows) or at cell- cell locations (closed white triangles) and uninternalized integrin at cell front (open white triangles). Images acquired by deconvolution microscopy and single Z-plane is shown. Bars, 20µm. Images are representative fields of view obtained in 3 independent experiments.
Table S2 from Combinations of Tyrosine Kinase Inhibitor and ERAD Inhibitor Promote Oxidative Stress–Induced Apoptosis through ATF4 and KLF9 in Medullary Thyroid Cancer
Authors: Bagheri-Yarmand Rozita , Sinha Krishna M. , Li Ling , Lu Yue , Cote Gilbert J. , Sherman Steven I. , Gagel Robert F.
Published: April 2023

Functional categ ories of ATF4 target genes.
Figure S4 from Combinations of Tyrosine Kinase Inhibitor and ERAD Inhibitor Promote Oxidative Stress–Induced Apoptosis through ATF4 and KLF9 in Medullary Thyroid Cancer
Authors: Bagheri-Yarmand Rozita , Sinha Krishna M. , Li Ling , Lu Yue , Cote Gilbert J. , Sherman Steven I. , Gagel Robert F.
Published: April 2023

ChIP -seq profiles of ATF4 target genes (DDIT3, MKNK2, LZTFL1, SIAH1) and H3K9Ac in MZCRC1 cells treated with eeyarestatin
Table S2. Top 10 Enriched Pathways Upregulated Following miR-200c Restoration from Reversal of Triple-Negative Breast Cancer EMT by miR-200c Decreases Tryptophan Catabolism and a Program of Immunosuppression
Authors: Rogers Thomas J. , Christenson Jessica L. , Greene Lisa I. , O'Neill Kathleen I. , Williams Michelle M. , Gordon Michael A. , Nemkov Travis , D'Alessandro Angelo , Degala Greg D. , Shin Jimin , Tan Aik-Choon , Cittelly Diana M. , Lambert James R. , Richer Jennifer K.
Published: April 2023

Extended list of genes associated with top 10 enriched pathways following GSEA analysis in BT549-TripZ-200c cells following miR-200c restoration.
Supplementary Figure 1 from Reversal of Triple-Negative Breast Cancer EMT by miR-200c Decreases Tryptophan Catabolism and a Program of Immunosuppression
Authors: Rogers Thomas J. , Christenson Jessica L. , Greene Lisa I. , O'Neill Kathleen I. , Williams Michelle M. , Gordon Michael A. , Nemkov Travis , D'Alessandro Angelo , Degala Greg D. , Shin Jimin , Tan Aik-Choon , Cittelly Diana M. , Lambert James R. , Richer Jennifer K.
Published: April 2023

Comparison of Gene Signatures following miR-200c Restoration in TNBC Cells.
Supplementary Methods from Divergent Activity of the Pseudogene PTENP1 in ER-Positive and Negative Breast Cancer
Authors: Yndestad Synnøve , Austreid Eilin , Skaftnesmo Kai Ove , Lønning Per Eystein , Eikesdal Hans Petter
Published: April 2023

Supplementary Methods
Supplementary Figure S4 from Divergent Activity of the Pseudogene PTENP1 in ER-Positive and Negative Breast Cancer
Authors: Yndestad Synnøve , Austreid Eilin , Skaftnesmo Kai Ove , Lønning Per Eystein , Eikesdal Hans Petter
Published: April 2023

Supplementary Figure S4
Supplemental Figures 1-7 from Integrative Epigenetic and Gene Expression Analysis of Renal Tumor Progression to Metastasis
Authors: Nam Hye-Young , Chandrashekar Darshan S. , Kundu Anirban , Shelar Sandeep , Kho Eun-Young , Sonpavde Guru , Naik Gurudatta , Ghatalia Pooja , Livi Carolina B. , Varambally Sooryanarayana , Sudarshan Sunil
Published: April 2023

1S: Principal component analysis (PCA) of the 44 experimental samples analyzed by array. S2. 2S: TCGA validation of up-regulated RCC associated genes. S3. TCGA validation of down-regulated RCC associated genes. Boxplots show expression level of each gene in 72 kidney normal samples and 533 kidney renal clear cell carcinoma samples. S4. Oncomine analysis of RCC associated genes. S5. Oncomine analysis of RCC associated genes. S6. Oncomine analysis of RCC associated genes. S7. Kaplan meier plots showing effect of gene expression level on overall survival of kidney renal clear cell carcinoma patients.
Supplementary Figure S3 from Divergent Activity of the Pseudogene PTENP1 in ER-Positive and Negative Breast Cancer
Authors: Yndestad Synnøve , Austreid Eilin , Skaftnesmo Kai Ove , Lønning Per Eystein , Eikesdal Hans Petter
Published: April 2023

Supplementary Figure S3
Supplementary figures S1-S7 from Integrative CAGE and DNA Methylation Profiling Identify Epigenetically Regulated Genes in NSCLC
Authors: Horie Masafumi , Kaczkowski Bogumil , Ohshima Mitsuhiro , Matsuzaki Hirotaka , Noguchi Satoshi , Mikami Yu , Lizio Marina , Itoh Masayoshi , Kawaji Hideya , Lassmann Timo , Carninci Piero , Hayashizaki Yoshihide , Forrest Alistair R.R. , Takai Daiya , Yamaguchi Yoko , Micke Patrick , Saito Akira , Nagase Takahide
Published: April 2023

Figure S1. Methylation and expression levels of genes in lung cancer and normal lung tissues. Figure S2. Expression levels of epi-markers in TCGA LUAD dataset. Figure S3. Expression levels of epi-markers in TCGA LUSC dataset. Figure S4. The signature of 22 epigenetically regulated genes in non-small cell lung cancer. Figure S5. ZENBU genome browser view of RP1-90G24.10 and AL022344.4. Figure S6. ZENBU genome browser view of MYEOV. Figure S7. Higher MYEOV expression is associated with poor prognosis in NSCLC.
Supplementary Figure S1 from Divergent Activity of the Pseudogene PTENP1 in ER-Positive and Negative Breast Cancer
Authors: Yndestad Synnøve , Austreid Eilin , Skaftnesmo Kai Ove , Lønning Per Eystein , Eikesdal Hans Petter
Published: April 2023

Supplementary Figure S1
Supplementary Tables S1-13 from Integrative CAGE and DNA Methylation Profiling Identify Epigenetically Regulated Genes in NSCLC
Authors: Horie Masafumi , Kaczkowski Bogumil , Ohshima Mitsuhiro , Matsuzaki Hirotaka , Noguchi Satoshi , Mikami Yu , Lizio Marina , Itoh Masayoshi , Kawaji Hideya , Lassmann Timo , Carninci Piero , Hayashizaki Yoshihide , Forrest Alistair R.R. , Takai Daiya , Yamaguchi Yoko , Micke Patrick , Saito Akira , Nagase Takahide
Published: April 2023

Table S1. Cell samples used for CAGE profiling. Table S2. Public datasets used in this study. Table S3. Differentially expressed promoters in FANTOM5 NSCLC cell lines. Table S4. Up-regulated/hypomethylated promoters in NSCLC cell lines. Table S5. Epi-markers in NSCLC. Table S6. Extended information for Table 2. Table S7. Univariate and multivariate Cox regression models of TCGA LUAD and LUSC datasets. Table S8. Gene expression profiling of SAECs treated with 5-aza-dC and TSA. Table S9. Up-regulated/hypomethylated genes in NSCLC cell lines that show up-regulation by 5-aza-dC and TSA in SAECs. Table S10. Promoters and methylation array probes that overlap nine families of repetitive elements. Table S11. Promoters and methylation array probes that overlap REP522 repetitive elements. Table S12. Transcripts commonly down-regulated by two different MYEOV siRNAs in A549 cells. Table S13. The relationship between expression of 22 epi-markers and EGFR/KRAS mutation in TCGA LUAD dataset.
Supplementary table 1-7 from Novel Aberrations Uncovered in Barrett's Esophagus and Esophageal Adenocarcinoma Using Whole Transcriptome Sequencing
Authors: Maag Jesper L.V. , Fisher Oliver M. , Levert-Mignon Angelique , Kaczorowski Dominik C. , Thomas Melissa L. , Hussey Damian J. , Watson David I. , Wettstein Antony , Bobryshev Yuri V. , Edwards Melanie , Dinger Marcel E. , Lord Reginald V.
Published: April 2023

Supplementary table 1 describes patient demographics and sample sequencing information Supplementary table 2 describes the top 20 differentially expressed genes, and the 12-gene signature. Supplementary table 3 shows the Discriminative performance of EAC driver genes in predicting the presence of EAC compared to BE with or without dysplasia. Supplementary table 4-5 describes the top 20 differentially expressed lncRNAs and repeat elements, respectively. Supplementary table 6 shows the association of genes in 12-signature with malignancies. Searched Pubmed: "Gene & Cancer" Supplementary table 7 shows the primers for lncRNA expression validation.
Supplementary Methods, Figure Legends, Figures 1 - 9, Tables 1 - 8 from Multiclonality and Marked Branched Evolution of Low-Grade Endometrioid Endometrial Carcinoma
Authors: Lazo de la Vega Lorena , Samaha Mia C. , Hu Kevin , Bick Nolan R. , Siddiqui Javed , Hovelson Daniel H. , Liu Chia-Jen , Carter Cody S. , Cho Kathleen R. , Sciallis Andrew P. , Tomlins Scott A.
Published: April 2023

Supplementary Methods, Figure Legends, Figures 1 - 9, Tables 1 - 8 from Multiclonality and Marked Branched Evolution of Low-Grade Endometrioid Endometrial Carcinoma
Suppementary methods from Novel Aberrations Uncovered in Barrett's Esophagus and Esophageal Adenocarcinoma Using Whole Transcriptome Sequencing
Authors: Maag Jesper L.V. , Fisher Oliver M. , Levert-Mignon Angelique , Kaczorowski Dominik C. , Thomas Melissa L. , Hussey Damian J. , Watson David I. , Wettstein Antony , Bobryshev Yuri V. , Edwards Melanie , Dinger Marcel E. , Lord Reginald V.
Published: April 2023

The supplementary methods describes the samples sequenced, the sequencing and bioinformatic analysis. It also describes the validation of lncRNA expression and methods for IHC.
Supplementary Table, Figure Legends from Molecular Response to Neoadjuvant Chemotherapy in High-Grade Serous Ovarian Carcinoma
Authors: Arend Rebecca C. , Londoño Angelina I. , Montgomery Allison M. , Smith Haller J. , Dobbin Zachary C. , Katre Ashwini A. , Martinez Alba , Yang Eddy S. , Alvarez Ronald D. , Huh Warner K. , Bevis Kerri S. , Straughn J. Michael , Estes Jacob M. , Novak Lea , Crossman David K. , Cooper Sara J. , Landen Charles N. , Leath Charles A.
Published: April 2023

Supplementary Table and Figure Legends
Supplementary Figure 5 from Molecular Response to Neoadjuvant Chemotherapy in High-Grade Serous Ovarian Carcinoma
Authors: Arend Rebecca C. , Londoño Angelina I. , Montgomery Allison M. , Smith Haller J. , Dobbin Zachary C. , Katre Ashwini A. , Martinez Alba , Yang Eddy S. , Alvarez Ronald D. , Huh Warner K. , Bevis Kerri S. , Straughn J. Michael , Estes Jacob M. , Novak Lea , Crossman David K. , Cooper Sara J. , Landen Charles N. , Leath Charles A.
Published: April 2023

Plasma cfDNA vs. Tumor DNA variant overlap.
Supplementary Figure 3 from Molecular Response to Neoadjuvant Chemotherapy in High-Grade Serous Ovarian Carcinoma
Authors: Arend Rebecca C. , Londoño Angelina I. , Montgomery Allison M. , Smith Haller J. , Dobbin Zachary C. , Katre Ashwini A. , Martinez Alba , Yang Eddy S. , Alvarez Ronald D. , Huh Warner K. , Bevis Kerri S. , Straughn J. Michael , Estes Jacob M. , Novak Lea , Crossman David K. , Cooper Sara J. , Landen Charles N. , Leath Charles A.
Published: April 2023

Heatmap. Pathway changes pre- and post-NACT.
Figure S2, B-D from Diffuse Intrinsic Pontine Glioma Cells Are Vulnerable to Mitotic Abnormalities Associated with BMI-1 Modulation
Authors: Senthil Kumar Shiva , Sengupta Satarupa , Zhu Xiaoting , Mishra Deepak Kumar , Phoenix Timothy , Dyer Lisa , Fuller Christine , Stevenson Charles B. , DeWire Mariko , Fouladi Maryam , Drissi Rachid
Published: April 2023

B, CCHMC-DIPG-1 cells treated with PTC596 (100 nM) were quantified for the number of γH2AX foci/cell at the indicated time points. C, Representative IF images and cell cycle analysis (with the indicated % of cells) of CCHMC-DIPG-2 cells treated with PTC596 (100 nM) for 24 hrs and 48 hrs. DNA damage (γH2AX in green) and apoptosis (cleaved caspase-3 in red) were evaluated and their respective quantification. DAPI (blue) represent nuclei. Error bars represent the SD from 4 different fields (n=150-400 cells/field). D, Immunoblot analysis of H3 S10-P in DIPG cells treated with indicated doses of PTC596 for 24 hrs. Total H3 served as loading control.
Figure S1 from Inhibition of Aurora-A Promotes CD8+ T-Cell Infiltration by Mediating IL10 Production in Cancer Cells
Authors: Han Jing , Jiang Zhen , Wang Chennan , Chen Xin , Li Rongqing , Sun Na , Liu Xiangye , Wang Hui , Hong Li , Zheng Kuiyang , Yang Jing , Ikezoe Takayuki
Published: April 2023

Figure S1 shows comparisons of tumor volumes and the population of intratumoral CD8+ T cells between the AURKAf/+ and AURKAf/+;VilCre+ mice treated with the control or alisertib.
Figure S6 from UNC-45A Is a Novel Microtubule-Associated Protein and Regulator of Paclitaxel Sensitivity in Ovarian Cancer Cells
Authors: Mooneyham Ashley , Iizuka Yoshie , Yang Qing , Coombes Courtney , McClellan Mark , Shridhar Vijayalakshmi , Emmings Edith , Shetty Mihir , Chen Liqiang , Ai Teng , Meints Joyce , Lee Michael K. , Gardner Melissa , Bazzaro Martina
Published: April 2023

Recombinant UNC-45A-GFP protein
Figure S1 from UNC-45A Is a Novel Microtubule-Associated Protein and Regulator of Paclitaxel Sensitivity in Ovarian Cancer Cells
Authors: Mooneyham Ashley , Iizuka Yoshie , Yang Qing , Coombes Courtney , McClellan Mark , Shridhar Vijayalakshmi , Emmings Edith , Shetty Mihir , Chen Liqiang , Ai Teng , Meints Joyce , Lee Michael K. , Gardner Melissa , Bazzaro Martina
Published: April 2023

Specificity of the UNC-45A antibody in immunohistochemistry
Figure S5 from The Tissue-Reconstructing Ability of Colon CSCs Is Enhanced by FK506 and Suppressed by GSK3 Inhibition
Authors: Ishida Ryo , Koyanagi-Aoi Michiyo , Oshima Nobu , Kakeji Yoshihiro , Aoi Takashi
Published: April 2023

Effects of calcineurin inhibitors and GSK3 inhibitors on 2nd V50 cells.
Supplementary Figure 1 (B). from Combining Homologous Recombination and Phosphopeptide-binding Data to Predict the Impact of BRCA1 BRCT Variants on Cancer Risk
Authors: Petitalot Ambre , Dardillac Elodie , Jacquet Eric , Nhiri Naima , Guirouilh-Barbat Josée , Julien Patrick , Bouazzaoui Isslam , Bonte Dorine , Feunteun Jean , Schnell Jeff A. , Lafitte Philippe , Aude Jean-Christophe , Noguès Catherine , Rouleau Etienne , Lidereau Rosette , Lopez Bernard S. , Zinn-Justin Sophie , Caputo Sandrine M.
Published: April 2023

Plot of the HR efficiencies measured after expression of either WT BRCA1 or VUS normalized to the HR efficiency after expression of WT BRCA1. The HR efficiencies are here plotted as a function of the position of the mutation in the BRCA1 sequence. Statistical significance was calculated using a one-side paired student t-test. To account for the multiple testing, the p-values were adjusted using the Benjamini-Horchberg method at a level a=0.05. HR efficiencies significantly different from the WT value are marked by asterisks. They are indicated by * if p < 0.05 and ** if p < 0.01. All 7 (likely) causal (noted LC or C) variants (in red) caused a significantly decreased HR efficiency whereas all 6 (likely) neutral (noted LN or N) variants (in blue) caused no significant HR difference. Twenty-four VUS of class 3 could not provide WT HR efficiency.
Supplemental Figure 1 from A Role for Tryptophan-2,3-dioxygenase in CD8 T-cell Suppression and Evidence of Tryptophan Catabolism in Breast Cancer Patient Plasma
Authors: Greene Lisa I. , Bruno Tullia C. , Christenson Jessica L. , D'Alessandro Angelo , Culp-Hill Rachel , Torkko Kathleen , Borges Virginia F. , Slansky Jill E. , Richer Jennifer K.
Published: April 2023

LAT1 expression in breast cancer clinical samples.
Supplementary Figure 4 from HIF1α Regulates mTOR Signaling and Viability of Prostate Cancer Stem Cells
Authors: Marhold Maximilian , Tomasich Erwin , El-Gazzar Ahmed , Heller Gerwin , Spittler Andreas , Horvat Reinhard , Krainer Michael , Horak Peter
Published: April 2023

Supplementary Figure 4. A) Two male eight-week-old NSG mice were injected in two ventral subcutaneous sites with 1x106 scrambled shRNA (scrCo) transduced and wild-type (wt) DU145 cells, respectively. Tumor volumes were calculated regularly and weights at the time of sacrifice as well as growth curves are shown. Time in days is shown on the x-axis and error bars indicate standard error of the mean (SEM). B) Three male eight-week-old NSG mice were injected in four ventral subcutaneous sites with 1x105 sorted CSC and non CSC subpopulations of shHIF1A as well as sorted control (scrCo) DU145 cells, respectively. Tumor volumes were calculated regularly and the growth curves of the CSC and non CSC populations from shHIF1A and scrCo DU145 cell lines are shown. Time in days is shown on the x-axis and error bars indicate SEM. C) Photograph of the xenografts arising from the four cell subpopulations in a representative NSG mouse at the time of sacrifice.
Supplementary Figure Legends 1-2 from S-Nitrosylation of EGFR and Src Activates an Oncogenic Signaling Network in Human Basal-Like Breast Cancer
Authors: Switzer Christopher H. , Glynn Sharon A. , Cheng Robert Y.-S. , Ridnour Lisa A. , Green Jeffrey E. , Ambs Stefan , Wink David A.
Published: April 2023

PDF file - 72K
Supplementary Figure 3 from HIF1α Regulates mTOR Signaling and Viability of Prostate Cancer Stem Cells
Authors: Marhold Maximilian , Tomasich Erwin , El-Gazzar Ahmed , Heller Gerwin , Spittler Andreas , Horvat Reinhard , Krainer Michael , Horak Peter
Published: April 2023

Supplementary Figure 3. Quantifications of western blot bands based on experiments described in detail in Figure 4. Ratio of phosphorylated to total protein amount is shown.
Supplementary Figure 2 from HIF1α Regulates mTOR Signaling and Viability of Prostate Cancer Stem Cells
Authors: Marhold Maximilian , Tomasich Erwin , El-Gazzar Ahmed , Heller Gerwin , Spittler Andreas , Horvat Reinhard , Krainer Michael , Horak Peter
Published: April 2023

Supplementary Figure 2. A) Analysis of basal prostate cancer stem cells in 5 different primary TRAMP tumors. We identified a distinct Lin-/CD49f+/Sca-1+ subpopulation in 19 (82,61%) of the 23 analyzed TRAMP mice. B) Quantified and normalized flow cytometry results of hypoxic CSC and non-CSC subpopulations of DU145 control (scrCo) and DU145 HIF1-α knockdown (shHIF1A) cells labeled with 7-AAD and Annexin V.
Supplementary Figure 1 from HIF1α Regulates mTOR Signaling and Viability of Prostate Cancer Stem Cells
Authors: Marhold Maximilian , Tomasich Erwin , El-Gazzar Ahmed , Heller Gerwin , Spittler Andreas , Horvat Reinhard , Krainer Michael , Horak Peter
Published: April 2023

Supplementary Figure 1. A) We identified a Lin-/CD49f+/Sca-1+ basal prostate stem cell population in wild-type C57BL/6 mice (left). HIF-1α mRNA was upregulated in sorted basal murine prostate stem cells (n=5, right). B) Treatment with increasing concentration of HIF-1α inhibitor leads to a dose dependent HIF-1α protein downregulation in hypoxic TRAMP-C1 cells. HIF-1α protein levels were assessed by immunoprecipitation and western blotting. IgG heavy chain (HC) is shown as a loading control for immunoprecipitation. Quantified and normalized protein levels are shown. C) HIF-1α shRNA expression in the DU145 cell line (shHIF1A) leads to reduced mRNA levels of HIF-1α and reduced expression of its target gene REDD1. Scrambled shRNA (scrCo) served as control. D) Knockdown of HIF-1α mRNA in the TRAMP-C1 murine cancer cell line. Scrambled shRNA (scrCo) served as control.
Supplementary Tables S1-S3 from Overexpression and Hypomethylation of Flap Endonuclease 1 Gene in Breast and Other Cancers
Authors: Singh Purnima , Yang Ming , Dai Huifang , Yu Dianke , Huang Qin , Tan Wen , Kernstine Kemp H. , Lin Dongxin , Shen Binghui
Published: April 2023

Supplementary Tables S1-S3 from Overexpression and Hypomethylation of Flap Endonuclease 1 Gene in Breast and Other Cancers
Supplementary Figures S1-S5, Tables S1-S3 from Survival Outcome and EMT Suppression Mediated by a Lectin Domain Interaction of Endo180 and CD147
Authors: Rodriguez-Teja Mercedes , Gronau Julian H. , Minamidate Ai , Darby Steven , Gaughan Luke , Robson Craig , Mauri Francesco , Waxman Jonathan , Sturge Justin
Published: April 2023

Supplementary Figures S1-S5, Tables S1-S3. Figure S1. Silencing Endo180 disrupts normal and benign PEC acini, but not PTC spheroids. Figure S2. None of the treatment used in culture affected cell cycle and/or cell viability. Figure S3. Epitope mapping of the Mouse monoclonal anti-Endo180 39.10. Figure S4. Targeted blockade of Endo180 does not alter spheroid formation by PTCs. Figure S5. Highly glycosylated CD147 can be precipitated from normal PEC lysates using CTLD4 as bait. Supplementary Table S1. Antibodies and reagents used for Immunoblotting, Immunofluorescence and Immunohistochemistry. Supplementary Table S2. NCLPC1/4 prostate cancer tissue microarray patient characteristics, N = 157. Supplementary Table S3. NCLPC1/4 prostate cancer tissue microarray - patient deaths [censored] after three, five, seven and ten years.
Supplementary Figures 1-8 from Mouse Tissues that Undergo Neoplastic Progression after K-Ras Activation Are Distinguished by Nuclear Translocation of phospho-Erk1/2 and Robust Tumor Suppressor Responses
Authors: Parikh Neha , Shuck Ryan L. , Nguyen Thuy-Ai , Herron Alan , Donehower Lawrence A.
Published: April 2023

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Supplementary Figure Legends 1-8 from Mouse Tissues that Undergo Neoplastic Progression after K-Ras Activation Are Distinguished by Nuclear Translocation of phospho-Erk1/2 and Robust Tumor Suppressor Responses
Authors: Parikh Neha , Shuck Ryan L. , Nguyen Thuy-Ai , Herron Alan , Donehower Lawrence A.
Published: April 2023

PDF file - 61K
Supplementary Data from Significance of Divergent Expression of Prostaglandin EP4 and EP3 Receptors in Human Prostate Cancer
Authors: Huang Hosea F. S. , Shu Ping , Murphy Thomas F. , Aisner Seena , Fitzhugh Valerie A. , Jordan Mark L.
Published: April 2023

Supplementary Data PDF file - 189K, supplementary data showing: a. Primers used for RT-PCR analysis of human prostate tissue and cell lines b. Primer pairs used for detection of human EP3 isoforms c. RT-PCR analysis of human EP3 isoforms present in malignant and benign human prostate cell lines
Supplementary Figure 5 from The Hedgehog Receptor Patched Functions in Multidrug Transport and Chemotherapy Resistance
Authors: Bidet Michel , Tomico Amandine , Martin Patrick , Guizouarn Hélène , Mollat Patrick , Mus-Veteau Isabelle
Published: April 2023

PDF file - 2.7MB, Schematic representation of action of Ptc and statins on chemotherapy resistance
Supplementary Figure 4 from The Hedgehog Receptor Patched Functions in Multidrug Transport and Chemotherapy Resistance
Authors: Bidet Michel , Tomico Amandine , Martin Patrick , Guizouarn Hélène , Mollat Patrick , Mus-Veteau Isabelle
Published: April 2023

PDF file - 392K, Gli1 is expressed in K562 cells
Supplementary Figure 3 from The Hedgehog Receptor Patched Functions in Multidrug Transport and Chemotherapy Resistance
Authors: Bidet Michel , Tomico Amandine , Martin Patrick , Guizouarn Hélène , Mollat Patrick , Mus-Veteau Isabelle
Published: April 2023

PDF file - 694K, hPtc expression reduces drugs accumulation in yeasts
Supplementary Table 1 from The Activation of MEK/ERK Signaling Pathway by Bone Morphogenetic Protein 4 to Increase Hepatocellular Carcinoma Cell Proliferation and Migration
Authors: Chiu Chiang-Yen , Kuo Kung-Kai , Kuo Tzu-Lei , Lee King-The , Cheng Kuang-Hung
Published: April 2023

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Supplementary Figure 5 from The Activation of MEK/ERK Signaling Pathway by Bone Morphogenetic Protein 4 to Increase Hepatocellular Carcinoma Cell Proliferation and Migration
Authors: Chiu Chiang-Yen , Kuo Kung-Kai , Kuo Tzu-Lei , Lee King-The , Cheng Kuang-Hung
Published: April 2023

PDF file - 188K
Figure S5 from Establishment of the First Well-differentiated Human Pancreatic Neuroendocrine Tumor Model
Authors: Benten Daniel , Behrang Yasmin , Unrau Ludmilla , Weissmann Victoria , Wolters-Eisfeld Gerrit , Burdak-Rothkamm Susanne , Stahl Felix R. , Anlauf Martin , Grabowski Patricia , Möbs Markus , Dieckhoff Jan , Sipos Bence , Fahl Martina , Eggers Corinna , Perez Daniel , Bockhorn Maximillian , Izbicki Jakob R. , Lohse Ansgar W. , Schrader Jörg
Published: April 2023

Cytokeratin expression profile NT-3 xenograft
Figure S4 from Establishment of the First Well-differentiated Human Pancreatic Neuroendocrine Tumor Model
Authors: Benten Daniel , Behrang Yasmin , Unrau Ludmilla , Weissmann Victoria , Wolters-Eisfeld Gerrit , Burdak-Rothkamm Susanne , Stahl Felix R. , Anlauf Martin , Grabowski Patricia , Möbs Markus , Dieckhoff Jan , Sipos Bence , Fahl Martina , Eggers Corinna , Perez Daniel , Bockhorn Maximillian , Izbicki Jakob R. , Lohse Ansgar W. , Schrader Jörg
Published: April 2023

SSTR expression original tumor
Figure S3 from Establishment of the First Well-differentiated Human Pancreatic Neuroendocrine Tumor Model
Authors: Benten Daniel , Behrang Yasmin , Unrau Ludmilla , Weissmann Victoria , Wolters-Eisfeld Gerrit , Burdak-Rothkamm Susanne , Stahl Felix R. , Anlauf Martin , Grabowski Patricia , Möbs Markus , Dieckhoff Jan , Sipos Bence , Fahl Martina , Eggers Corinna , Perez Daniel , Bockhorn Maximillian , Izbicki Jakob R. , Lohse Ansgar W. , Schrader Jörg
Published: April 2023

Growth characteristics of BON, AGP and NT-3
Figure S2 from Establishment of the First Well-differentiated Human Pancreatic Neuroendocrine Tumor Model
Authors: Benten Daniel , Behrang Yasmin , Unrau Ludmilla , Weissmann Victoria , Wolters-Eisfeld Gerrit , Burdak-Rothkamm Susanne , Stahl Felix R. , Anlauf Martin , Grabowski Patricia , Möbs Markus , Dieckhoff Jan , Sipos Bence , Fahl Martina , Eggers Corinna , Perez Daniel , Bockhorn Maximillian , Izbicki Jakob R. , Lohse Ansgar W. , Schrader Jörg
Published: April 2023

Neuroendocrine Phenotype and mutational status NT-3
Figure S1 from Establishment of the First Well-differentiated Human Pancreatic Neuroendocrine Tumor Model
Authors: Benten Daniel , Behrang Yasmin , Unrau Ludmilla , Weissmann Victoria , Wolters-Eisfeld Gerrit , Burdak-Rothkamm Susanne , Stahl Felix R. , Anlauf Martin , Grabowski Patricia , Möbs Markus , Dieckhoff Jan , Sipos Bence , Fahl Martina , Eggers Corinna , Perez Daniel , Bockhorn Maximillian , Izbicki Jakob R. , Lohse Ansgar W. , Schrader Jörg
Published: April 2023

Morphology of NT-3
Supplementary Tables S1-S2, Figures S1-S4 from Histone Methyltransferase hSETD1A Is a Novel Regulator of Metastasis in Breast Cancer
Authors: Salz Tal , Deng Changwang , Pampo Christine , Siemann Dietmar , Qiu Yi , Brown Kevin , Huang Suming
Published: April 2023

Supplementary Tables S1-S2, Figures S1-S4. Table S1. RT-PCR Primer Sequence Table S2. ChIP Primer Sequence Figure S1. Expression of TrxG in breast cancer samples. S2. hSETD1A Knockdown in MDA-MB-231 using independent shRNA constructs. S3. hSETD1A mRNA level predicts clinical outcomes for lymph-node positive breast cancer patients. S4. H3K4me3 and hSETD1A are correspondingly enriched at MMP promoters in breast cancer cells compared to epithelial breast cells.
Supplementary Tables S1-S8 from IGF2 Autocrine-Mediated IGF1R Activation Is a Clinically Relevant Mechanism of Osimertinib Resistance in Lung Cancer
Authors: Manabe Tadashi , Yasuda Hiroyuki , Terai Hideki , Kagiwada Harumi , Hamamoto Junko , Ebisudani Toshiki , Kobayashi Keigo , Masuzawa Keita , Ikemura Shinnosuke , Kawada Ichiro , Hayashi Yuichiro , Fukui Kazuhiko , Horimoto Katsuhisa , Fukunaga Koichi , Soejima Kenzo
Published: April 2023

Supplementary Tables includes 8 tables. Supplementary Table S1: Patient-derived cancer cells: Patient and model characteristics. Supplementary Table S2: List of primer sequences used in quantitative RT-PCR for gene expression analysis. Supplementary Table S3: List of primer sequences used in quantitative PCR for DNA copy number determination. Supplementary Table S4: The list of pathways detected by the protein array and their calculated value scores. Supplementary Table S5: The combination of osimertinib and linsitinib demonstrated a synergistic anti-proliferative effect in PC9AZDR cells. Supplementary Table S6: Mutations detected by target sequence in KOLK43 cells. Supplementary Table S7: The combination of osimertinib and linsitinib demonstrated synergistic anti-proliferative effect in KOLK43 cells. Supplementary Table S8: IGF2 is upregulated in human EGFR-mutant NSCLC with resistance to EGFR-TKI.
Supplementary Figure Legends from CDK4/6 Therapeutic Intervention and Viable Alternative to Taxanes in CRPC
Authors: Stice James P. , Wardell Suzanne E. , Norris John D. , Yllanes Alexander P. , Alley Holly M. , Haney Victoria O. , White Hannah S. , Safi Rachid , Winter Peter S. , Cocce Kimberly J. , Kishton Rigel J. , Lawrence Scott A. , Strum Jay C. , McDonnell Donald P.
Published: April 2023

Contains legends to supplementary figures.
Supplementary Figure 4 from Alpha-Melanocyte–Stimulating Hormone Suppresses Oxidative Stress through a p53-Mediated Signaling Pathway in Human Melanocytes
Authors: Kadekaro Ana Luisa , Chen Juping , Yang Jennifer , Chen Shuna , Jameson Joshua , Swope Viki B. , Cheng Tan , Kadakia Madhavi , Abdel-Malek Zalfa
Published: April 2023

PDF file - 15MB, Hydrogen peroxide generation in melanocytes irradiated with 105 mJ/cm2 UVR in the presence and absence of pifithrin-alpha
Supplementary Figures 1-7 from Inhibition of the Hedgehog Pathway Targets the Tumor-Associated Stroma in Pancreatic Cancer
Authors: Hwang Rosa F. , Moore Todd T. , Hattersley Maureen Mertens , Scarpitti Meghan , Yang Bin , Devereaux Erik , Ramachandran Vijaya , Arumugam Thiruvengadam , Ji Baoan , Logsdon Craig D. , Brown Jeffrey L. , Godin Robert
Published: April 2023

PDF file - 705K, S1. Identification of potent Hh pathway inhibitors. S2. SHH and IHH expression and effects on HPSCs. S3. Effect of AZD8542 in an orthotopic model of pancreatic cancer with luciferase-labeled MPanc96 cells co-injected with HPSCs. S4. AZD8542 is a potent in vivo inhibitor of the Hh pathway. S5. In vivo efficacy of AZD8542 in an HT29/MEF co-implant model. S6. Effect of AZD8542 in an orthotopic model of pancreatic cancer with luciferase-labeled Bxpc3 cells coinjected with HPSCs. S7. IVIS imaging of luciferase in an orthotopic model of PDAC
Supplementary Figure 1 from Alpha-Melanocyte–Stimulating Hormone Suppresses Oxidative Stress through a p53-Mediated Signaling Pathway in Human Melanocytes
Authors: Kadekaro Ana Luisa , Chen Juping , Yang Jennifer , Chen Shuna , Jameson Joshua , Swope Viki B. , Cheng Tan , Kadakia Madhavi , Abdel-Malek Zalfa
Published: April 2023

PDF file - 3.8MB, Western blott analysis - Absence of p63 isoforms in human melanocytes
Supplementary Table 3 from MicroRNAs Associated with Mitogen-Activated Protein Kinase in Human Pancreatic Cancer
Authors: Ikeda Yushi , Tanji Etsuko , Makino Naohiko , Kawata Sumio , Furukawa Toru
Published: April 2023

XLS file - 173K
Supplementary Video 1 from FES Kinase Promotes Mast Cell Recruitment to Mammary Tumors via the Stem Cell Factor/KIT Receptor Signaling Axis
Authors: Kwok Ester , Everingham Stephanie , Zhang Shengnan , Greer Peter A. , Allingham John S. , Craig Andrew W.B.
Published: April 2023

AVI file - 24K, BMMCs from fes+/+ and fes-/- mice were loaded with CellTracker green or CellTracker orange, respectively, prior to mixing and addition to fibronectin-coated coverslips with an SCF-embedded agarose drop. Time lapse microscopy was then performed for 8-18 hours
Supplementary Table 2 from MicroRNAs Associated with Mitogen-Activated Protein Kinase in Human Pancreatic Cancer
Authors: Ikeda Yushi , Tanji Etsuko , Makino Naohiko , Kawata Sumio , Furukawa Toru
Published: April 2023

XLS file - 19K
Supplementary Table 5 from The Lymphotactin Receptor Is Expressed in Epithelial Ovarian Carcinoma and Contributes to Cell Migration and Proliferation
Authors: Kim Mijung , Rooper Lisa , Xie Jia , Rayahin Jamie , Burdette Joanna E. , Kajdacsy-Balla Andre A. , Barbolina Maria V.
Published: April 2023

PDF file - 66K, Expression of XCR1 in multiple organ metastatic cancer specimens
Table S4 from Metastasis-Specific Gene Expression in Autochthonous and Allograft Mouse Mammary Tumor Models: Stratification and Identification of Targetable Signatures
Authors: Ross Christina , Szczepanek Karol , Lee Maxwell , Yang Howard , Peer Cody J. , Kindrick Jessica , Shankarappa Priya , Lin Zhi-Wei , Sanford Jack D. , Figg William D. , Hunter Kent W.
Published: April 2023

IPA analysis of common metastasis specific genes
Table S3 from Metastasis-Specific Gene Expression in Autochthonous and Allograft Mouse Mammary Tumor Models: Stratification and Identification of Targetable Signatures
Authors: Ross Christina , Szczepanek Karol , Lee Maxwell , Yang Howard , Peer Cody J. , Kindrick Jessica , Shankarappa Priya , Lin Zhi-Wei , Sanford Jack D. , Figg William D. , Hunter Kent W.
Published: April 2023

Metastasis specific gene expression analyses
Supplementary Figure 2 from The Lymphotactin Receptor Is Expressed in Epithelial Ovarian Carcinoma and Contributes to Cell Migration and Proliferation
Authors: Kim Mijung , Rooper Lisa , Xie Jia , Rayahin Jamie , Burdette Joanna E. , Kajdacsy-Balla Andre A. , Barbolina Maria V.
Published: April 2023

PDF file - 225K, Immunohistochemical analysis of XCR1 expression in distant metastasis originating from different primary solid tumors: a) bladder to pelvic cavity, b) breast to armpit, c) cervix to pelvic cavity, d) colon to liver, e) endometrium to abdominal cavity, f) esophagus to mediastinum, g) kidney to right upper arm, h) larynx to neck, i) liver to abdominal cavity, j) lung to cerebellum, k) skin of left thigh to right groin, l) pancreas to omentum, m) prostate to costal bone, n) duodenum to mesentery, o) rectum to ovary, p) small intestine to mesentery, q) stomach to abdominal wall, r) thyroid to left frontal lobe, s) skin of penis to right groin, and t) lung to jejunum. Rabbit anti-human XCR1 antibodies (Novus Biologicals) were used at a 1:50 dilution; biotin-conjugated anti-rabbit antibodies were used at a 1:500 dilution. Brown - XCR1, blue - haemotoxylin. Images were generated using the Aperio ScanScope digital slide scanner. Bar = 50 micron.
Figure S2 from Metastasis-Specific Gene Expression in Autochthonous and Allograft Mouse Mammary Tumor Models: Stratification and Identification of Targetable Signatures
Authors: Ross Christina , Szczepanek Karol , Lee Maxwell , Yang Howard , Peer Cody J. , Kindrick Jessica , Shankarappa Priya , Lin Zhi-Wei , Sanford Jack D. , Figg William D. , Hunter Kent W.
Published: April 2023

Workflow and aRT-PCR results
Figure S1 from Metastasis-Specific Gene Expression in Autochthonous and Allograft Mouse Mammary Tumor Models: Stratification and Identification of Targetable Signatures
Authors: Ross Christina , Szczepanek Karol , Lee Maxwell , Yang Howard , Peer Cody J. , Kindrick Jessica , Shankarappa Priya , Lin Zhi-Wei , Sanford Jack D. , Figg William D. , Hunter Kent W.
Published: April 2023

ImmQuant results comparison of allograft versus autochthonous tumors
Supplementary Figures 1-4 from FAS1 Domain Protein Inhibits VEGF165-Induced Angiogenesis by Targeting the Interaction between VEGFR-2 and αvβ3 Integrin
Authors: Nam Ju-Ock , Son Hye-Nam , Jun Eunsung , Cha Kiweon , Lee Byung-Heon , Park Rang-Woon , Kim In-San
Published: April 2023

PDF file - 259K, S1 Cell cytotoxicity assay. S2 FAS1-domain protein inhibits VEGF165-induced 9 association of alphavbeta3 integrin with VEGFR-2 and VEGFR-2 phosphorylation via alphavbeta3 10 integrin. S3 FAS1-domain protein inhibits both vitronectin- and 32 collagen-i 1 nduced VEGFR-2 phosphorylation and association of alphavbeta3 integrin with 2 VEGFR-2. S4 Serum-starved VEGFR-2/beta5/HEK293 cells were plated on collagen I-coated in serum-free 16 medium supplemented with FAS1-domain protein (10 muM) for 30 min, followed by 17 stimulation for 5 min with VEGF165 (20 ng/ml) or vehicle alone
Supplementary Figures 1-2 from A Vascular Model of Tsc1 Deficiency Accelerates Renal Tumor Formation with Accompanying Hemangiosarcomas
Authors: Leech Jarrett D. , Lammers Stephen H.T. , Goldman Sam , Auricchio Neil , Bronson Roderick T. , Kwiatkowski David J. , Sahin Mustafa
Published: April 2023

Supplementary Figures 1-2: Supplementary Figure 1. Genotyping Tsc1cc DARPP Cre mice. Separate Polymerase Chain Reaction experiments showing Darpp-32 Cre allele genotyping (A) and TSC1 genotyping (B) with cc representing the conditional null genotype and cw the wild type genotype. Supplementary Figure 2. High magnification of paw and renal morphology. A-C) Hematoxylin and Eosin staining of mutant 17 week old mouse paw and kidney with arrows indicating boundaries between non-tumor and tumor tissue as characterized by vascularized tumor (paw hemangiosarcoma) or hyperplastic epithelial linings (kidney cystadenoma). A) 2X magnification of paw with identifiable hemangiosarcomas surrounding phalanges II through IV. B) 20X magnification showing cortical renal pathology. C) 60X magnification showing glomerular apparatus surrounded by hyperplastic epithelial tumor tissue. 2X scale bar = 1mm, 20X scale bar = 100 um, 60X scale bar = 33 um.
Supplementary Figure 1 from Ligand Binding Promotes CDK-Dependent Phosphorylation of ER-Alpha on Hinge Serine 294 but Inhibits Ligand-Independent Phosphorylation of Serine 305
Authors: Held Jason M. , Britton David J. , Scott Gary K. , Lee Elbert L. , Schilling Birgit , Baldwin Michael A. , Gibson Bradford W. , Benz Christopher C.
Published: April 2023

PDF file - 132K, S1 Differential phosphorylation of ERalpha following E2 or growth factor stimulation. S2 Stable isotope dilution MRM (SID-MRM) quantitation of Ser294 and Ser167 using heavy-labeled synthetic peptides
Supplementary Figures 1-7 and Table S1 from Lysine-Specific Demethylase 1 Mediates AKT Activity and Promotes Epithelial-to-Mesenchymal Transition in PIK3CA-Mutant Colorectal Cancer
Authors: Miller Samuel A. , Policastro Robert A. , Savant Sudha S. , Sriramkumar Shruthi , Ding Ning , Lu Xiaoyu , Mohammad Helai P. , Cao Sha , Kalin Jay H. , Cole Philip A. , Zentner Gabriel E. , O'Hagan Heather M.
Published: April 2023

Supplementary Table S1: Mutational status of common oncogenes in CRC and STAD cell lines. Supplementary Figure S1: Mutant PIK3CA does not regulate LSD1 expression. Supplementary Figure S2: DNase signal is not generally depleted at TSS's, and LSD1 does not reduce global H3K4me2. Supplementary Figure S3: RCOR1 is generally not upregulated in PIK3CA mutant versus WT in cancers arising from non-gastrointestinal tissues, and generally does not correlate with LSD1 expression. Supplementary Figure S4: LSD1 KO reduces cell viability over time in HT29 but not SW480 cells. Supplementary Figure S5: Inhibiting AKT does not reduce Snail protein level in PIK3CA WT or kinase domain mutant cells, and the LSD1-AKT-GSK3b-Snail axis is context dependent. Supplementary Figure S6: LSD1 is upregulated in PIK3CA mutants classified as CRC subtype CMS4, where C2 domain mutations are also most common. Supplementary Figure S7: EGF treatment causes morphological changes in HT29 WT but not LSD1 KO cells.
Supplementary Table 1, Figures 1-3 from Pten Regulates Aurora-A and Cooperates with Fbxw7 in Modulating Radiation-Induced Tumor Development
Authors: Kwon Yong-Won , Kim Il-Jin , Wu Di , Lu Jing , Stock William A. , Liu Yueyong , Huang Yurong , Kang Hio Chung , DelRosario Reyno , Jen Kuang-Yu , Perez-Losada Jesus , Wei Guangwei , Balmain Allan , Mao Jian-Hua
Published: April 2023

PDF file - 222K
Supplementary Table 4 from Suppression of G-protein–Coupled Receptor Kinase 3 Expression Is a Feature of Classical GBM That Is Required for Maximal Growth
Authors: Woerner B. Mark , Luo Jingqin , Brown Kristin R. , Jackson Erin , Dahiya Sonika M. , Mischel Paul , Benovic Jeffrey L. , Piwnica-Worms David , Rubin Joshua B.
Published: April 2023

PDF file - 116KB
Supplementary Appendix including cell line RRIDs and supplementary figures from Ceramide Analogue SACLAC Modulates Sphingolipid Levels and MCL-1 Splicing to Induce Apoptosis in Acute Myeloid Leukemia
Authors: Pearson Jennifer M. , Tan Su-Fern , Sharma Arati , Annageldiyev Charyguly , Fox Todd E. , Abad Jose Luis , Fabrias Gemma , Desai Dhimant , Amin Shantu , Wang Hong-Gang , Cabot Myles C. , Claxton David F. , Kester Mark , Feith David J. , Loughran Thomas P.
Published: April 2023

Figure S1. SACLAC reduces AC activity at lower doses than LCL204. Figure S2. SACLAC treatment increases production of multiple ceramide species in human AML cell lines. Figure S3. SACLAC is more toxic to AML cells than normal cells. Figure S4. SACLAC mechanism of action is consistent in multiple human AML cell lines. Figure S5. Exogenous ceramides induce apoptosis in human AML cell lines. Figure S6. AC knockdown alters splicing of Mcl-1 and Bim in HL-60/VCR cells via regulation of SF3B1. Figure S7. Baseline apoptosis varies based on type of electroporated content. Figure S8. Proposed model of SACLAC mechanism of action. Figure S9. IV administration of SACLAC improves delivery and lipids are shifted in mice.
Supplementary Figures 1-8 from STAT3-RANTES Autocrine Signaling Is Essential for Tamoxifen Resistance in Human Breast Cancer Cells
Authors: Yi Eun Hee , Lee Chang Seok , Lee Jin-Ku , Lee Young Ju , Shin Min Kyung , Cho Chung-Hyun , Kang Keon Wook , Lee Jung Weon , Han Wonshik , Noh Dong-Young , Kim Yong-Nyun , Cho Ik-Hyun , Ye Sang-kyu
Published: April 2023

PDF file - 1.2MB, Supplementary Fig. S1. MCF-7 cells were cultured in the absence or presence of tamoxifen for 9 months. Supplementary Fig. S2. Levels of cytokines secreted by MCF-7 and TRM-7 cells, as determined using a cytokine array. Supplementary Fig. S3. Neutralization of secreted RANTES was confirmed by ELISA. Supplementary Fig. S4. (A) RANTES siRNA constructs evaluated by real-time PCR. (B) STAT3 siRNA constructs evaluated by real-time PCR. The siRNA that resulted in the greatest knockdown of each gene was selected. Columns, means of triplicate samples; bars, S.D. *, P < 0.01. (C) RANTES and total STAT3 mRNA levels in siRNA-transfected MCF-7 and TRM-7 cells were determined by RT-PCR. Supplementary Fig. S5. (A) Recombinant RANTES (50 ng/ml)-treated MCF-7 cells were subjected to western blotting. (B) MCF-7 cells were cultured in the absence or presence of recombinant RANTES (50 ng/ml) for 2 weeks and their sensitivities to actinomycin D and (C) cisplatin were determined. Supplementary Fig. S6. (A), (B) T47D cells were cultured in the absence or presence of recombinant RANTES (50 ng/ml) for 2 weeks. Supplementary Fig. S7. (A), (B) pY-STAT3 and RANTES expression levels in primary ERa-positive breast cancer (n = 8) and tamoxifen treated ERa-positive breast cancer (n = 8) cells were evaluated by immunohistochemical staining. Supplementary Fig. S8. (A) ERa expression levels in MCF-7 and TRM-7 cells. (B) ERK and p38 activation in MCF-7 and TRM-7 cells. (C) RANTES and RANTES receptor (CCR1, 3, 5) mRNA levels were determined by real-time PCR and RT-PCR
Supplementary Figure 1 from Role of Neurofilament Light Polypeptide in Head and Neck Cancer Chemoresistance
Authors: Chen Baishen , Chen Ju , House Michael G. , Cullen Kevin J. , Nephew Kenneth P. , Guo Zhongmin
Published: April 2023

PDF file - 127K
Supplementary Figure 2 from Suppression of G-protein–Coupled Receptor Kinase 3 Expression Is a Feature of Classical GBM That Is Required for Maximal Growth
Authors: Woerner B. Mark , Luo Jingqin , Brown Kristin R. , Jackson Erin , Dahiya Sonika M. , Mischel Paul , Benovic Jeffrey L. , Piwnica-Worms David , Rubin Joshua B.
Published: April 2023

PDF file - 7422KB
Video 2 from Complex Rab4-Mediated Regulation of Endosomal Size and EGFR Activation
Authors: Tubbesing Kate , Ward Jamie , Abini-Agbomson Raymond , Malhotra Aditi , Rudkouskaya Alena , Warren Janine , Lamar John , Martino Nina , Adam Alejandro P. , Barroso Margarida
Published: April 2023

Video S2. Corresponds to Figure 2A. Created for visualization of MDAMB231 enlarged endosome with EEA1 (red) and Tf (green).