bluuebunny/crossref_metadata_2025 huggingface dataset:
“I need more knowledge”: Qualitative analysis of oncology providers’ experiences with sexual and gender minority patients
Authors: Tamargo Christina L. , Mitchell Edith P. , Wagner Lynne , Simon Melissa A. , Carlos Ruth C. , Giantonio Bruce J. , Schabath Matthew B. , Quinn Gwendolyn P.
Published: August 2022

Background

While societal acceptance for sexual and gender minority (SGM) individuals is increasing, this group continues to face barriers to quality healthcare. Little is known about clinicians’ experiences with SGM patients in the oncology setting. To address this, a mixed method survey was administered to members of the ECOG-ACRIN Cancer Research Group.

Materials and methods

We report results from the open-ended portion of the survey. Four questions asked clinicians to describe experiences with SGM patients, reservations in caring for them, suggestions for improvement in SGM cancer care, and additional comments. Data were analyzed using content analysis and the constant comparison method.

Results

The majority of respondents noted they had no or little familiarity with SGM patients. A minority of respondents noted experience with gay and lesbian patients, but not transgender patients; many who reported experience with transgender patients also noted difficulty navigating the correct use of pronouns. Many respondents also highlighted positive experiences with SGM patients. Suggestions for improvement in SGM cancer care included providing widespread training, attending to unique end-of-life care issues among SGM patients, and engaging in efforts to build trust.

Conclusion

Clinicians have minimal experiences with SGM patients with cancer but desire training. Training the entire workforce may improve trust with, outreach efforts to, and cancer care delivery to the SGM community.
Musicokinetic and exercise therapies decrease the depression level of elderly patients undergoing post-stroke rehabilitation: The moderating effect of health regulatory focus
Authors: Zhao Li , Lyu Xiaokang , Jiang He , Gao Xinhai
Published: August 2022

This study aims to investigate the impact of musicokinetic and exercise therapies on the depression level of elderly patients undergoing post-stroke rehabilitation and its possible moderators, the promotion focus (i.e., achieve gains) and prevention focus (i.e., avoid losses or non-gains), which are the two motivational orientations of health regulatory focus. An eight-week randomized controlled trial was employed. Sixty-five elderly patients undergoing post-stroke rehabilitation in a hospital in Shanghai, China. Patients were randomly assigned to the musicokinetic (n = 32) therapy group or the exercise (n = 33) therapy group. The Mini-mental State Examination Scale measuring the patients’ cognitive functions was used to screen participants. The Hamilton Depression Rating Scale and the Health Regulatory Focus Scale were applied to assess their levels of depression and health regulatory focus on weeks 0, 4, and 8, respectively. The musicokinetic therapy had a significantly better effect than the exercise therapy for individuals who had a lower level of prevention focus, whereas the exercise therapy had a significantly better effect than the musicokinetic therapy for individuals who had a higher level of prevention focus. Musicokinetic therapy and exercise therapy were both effective in decreasing post-stroke depression for elderly patients. But it is important to choose an appropriate type of therapy per the health regulatory focus of elderly patients with post-stroke rehabilitation.
Paranormal belief and well-being: The moderating roles of transliminality and psychopathology-related facets
Authors: Dagnall Neil , Denovan Andrew , Drinkwater Kenneth Graham , Escolà-Gascón Álex
Published: August 2022

Evaluation of prior research suggests that belief in the paranormal is more likely to be associated with negative psychological functioning, when presented alongside cognitive-perceptual factors that askew thinking and insight. The current study examined this notion using a sample of 3,084 participants (1,382 males, 1,693 females, nine non-binary). Respondents completed self-report measures assessing Paranormal Belief, Transliminality, psychopathology-related characteristics (Schizotypy and Manic-Depressive Experience), and well-being (Perceived Stress and Somatic Complaints). Responses were analysed via correlations and moderation. Paranormal Belief correlated positively with Transliminality, psychopathology-related measures, Perceived Stress, and Somatic Complaints. Moderation analyses revealed that Transliminality and psychopathology-related variables (i.e., the Unusual Experiences and Cognitive Disorganisation subscales of schizotypy, and Manic-Depressive Experience) interacted with Paranormal Belief in complex ways and were allied to higher scores on negative well-being outcomes. This indicated that within paranormal believers, Transliminality and specific psychopathology-related variables in combination predicted susceptibility to negative well-being outcomes.
Association of social support with negative emotions among Chinese adolescents during Omicron-related lockdown of Shenzhen City: The roles of rumination and sleep quality
Authors: Guo Tianyou , Zhang Zhihao , Taylor Alyx , Hall Daniel L. , Yeung Albert S. , Kramer Arthur F. , Zou Liye
Published: August 2022

Objective

Adolescents are likely to suffer from negative emotions such as depression and anxiety due to the rapid development of biological, cognitive and social changes. Previous studies have indicated possible risk (rumination) and protective (good social support and high sleep quality) factors for depression and anxiety among this age group. The present study is the first to investigate the association between social support and negative emotions during the Outbreak of Omicron variant, on this basis, to further determine the mediating role of rumination and sleep quality on this link.

Method

A total of 1,065 Chinese middle- and high-school students (51.5% female, Mage = 13.80, SD = 1.20) completed a psychosocial battery, including the Social Support Rating Scale (SSRS), the Pittsburgh Sleep Quality Index (PSQI), the Ruminative Responses Scale (RRS), the Depression Anxiety Stress Scale (DASS). Serial multiple mediation analysis was conducted using PROCESS macro based on SPSS.

Results

Social support, rumination, and sleep quality were significantly negatively correlated with negative emotional states (Ps < 0.05). Further, rumination and sleep quality were found to partially mediate the relationship between social support and negative emotional states.

Conclusions

For early detection and prevention of depression and anxiety, providing sufficient social support is necessary for adolescents, because rumination and sleep problems are reported during stressful periods, such as the COVID-19 pandemic.
Vulnerable populations during COVID-19 response: Health-related quality of life among Chinese population and its influence due to socio-demographic factors and loneliness
Authors: Wong Eliza Lai-Yi , Li Jia , Yuen Shannon , Lai Angel Hor-Yan , Cheung Annie Wai-Ling , Yau Peter Sen-Yung , Yeoh Eng-Kiong
Published: August 2022

Background

Infection control policy affected people's wellbeing during the COVID-19 pandemic, especially those vulnerable populations. This study aimed to compare the health-related quality of life (HRQoL) of the Hong Kong (HK) Chinese population under the pandemic with the normative profiles and explore its influencing factors, including socio-demographic characteristics, loneliness, and the interaction between them.

Methods

A cross-sectional questionnaire survey (301 online and 202 in-person) was conducted between June and December 2020 among the adult Chinese population during the 2nd wave of COVID-19 in HK. HRQoL was measured by a Hong Kong validated EQ-5D-5L instrument (EQ-5D-5L HK). Loneliness was measured by a single-item question regarding the frequency of the participants reporting feeling lonely and their subjective social status was measured by the MacArthur Scale of Subjective Social Status. A series of Tobit regressions was conducted. The interaction terms between socio-demographics and loneliness were also examined to decide their association with HRQoL.

Results

A total of 503 responses were collected. The level of HRQoL of the respondents was significantly lower than the referred norms profile among the local general population. The findings identified that younger age, single, a higher subjective social status, and a lower level of loneliness were significantly associated with better HRQoL. Moreover, age and marital status were significant moderators in the relationship between loneliness and HRQoL.

Conclusion

The present study found that some population groups face additional vulnerabilities during the pandemic in terms of declined HRQoL. In addition, reducing loneliness can protect the HRQoL during the pandemic, especially among older people. This article provides useful information for policy-makers to design and promote effective services or provide education to improve the connection of people and recover from the global pandemic.
Food handler's level of COVID-19 prevention practice and preparedness of food and drinking establishments to combat the pandemic in Eastern Ethiopia
Authors: Habte Sisay , Debella Adera , Abdeta Tilahun , Birhanu Abdi , Balis Bikila , Eshetu Bajrond , Bekele Habtamu
Published: August 2022

Background

Food and drinking establishments are potential hotspots for the spread of coronavirus. Food handler's have a higher risk of contracting the disease and transmitting it to others. The aim of this study was to assess the food handler's level of preventive practices toward COVID-19 and the preparedness of food and drinking establishments to tackle the pandemic in Eastern Ethiopia.

Methods

The cross-sectional study design was conducted from September 1 to 30, 2020. A stratified sampling technique was used to select 276 licensed public food and drinking establishments and a simple random sampling technique was employed to select 422 food handlers from the selected establishments. A face-to-face interview and checklist-based observation were used to collect data. The collected data were entered into Epidata 3.1 and exported to STATA version 16 for analysis. Binary logistic regression analysis were conducted to identify factors associated with COVID-19 preventive practice. Statistical significance was declared at p < 0.05.

Results

About 406 food handler's participated in this study, making the response rate 96.2%. The study showed that 38.4% of study participants (95% CI: 33.5, 43.1) had good preventive practices for COVID-19. Only 10.5% of food and drinking establishments fulfilled all requirements to prevent COVID-19 transmission. Being male [AOR = 0.61, 95% CI(0.61, (0.39, 0.93)], attending secondary education [AOR = 2.20, (95% CI: 1.37, 3.53)], having a favorable attitude toward COVID-19 [AOR = 1.89, (95% CI: 1.22, 2.95)], and having good knowledge about COVID-19 [AOR = 1.78, (95% CI: 1.13, 2.81)] were significantly associated with the level of COVID-19 preventive practices.

Conclusion

The level of good COVID-19 preventive practice was found to be low among the food handler's. Only one in ten food and drink establishments fulfilled the national guideline for preventing COVID-19 transmission. Being male, attending secondary education, having knowledge about COVID-19, and having a favorable attitude toward COVID-19 were significantly associated with good COVID-19 preventive practices. A vibrant guideline on prevention practices should be in place at all establishments, and compliance should be monitored. Local health office experts should take comprehensive measures to make all food and drinking establishments accountable for practicing all preventive measures.
Iranian nurses' knowledge and attitude toward complementary and alternative medicines: Is there any relation with quality of nursing care?
Authors: Dehghan Mahlagha , Namjoo Zakieh , Jafari Mahlasadat , Kordestani Ghazaleh , Tabebordbar Nazanin , Payam Fatemeh , Zakeri Mohammad Ali , Mokhtarabadi Sima
Published: August 2022

Background

Nurses play an important role in advising and guiding patients on effective treatments, and in this situation, it is better to be aware of complementary and alternative medicine (CAM) as well as the effects and side effects of different procedures. In addition, the quality of nursing care is directly related to the nurse's knowledge of the different treatments and preventions of different diseases and conditions. The present study aimed to investigate Iranian nurses' knowledge and attitude toward complementary and alternative medicines (CAMs) as well as their correlation with the quality of patient care.

Materials and methods

This cross-sectional study included 267 clinical nurses from three hospitals in southern Iran. The participants were recruited using convenience sampling methods in 2020–2021. A demographic questionnaire, knowledge and attitude toward CAM questionnaires, and the Quality Patient Care Scale were used to collect data.

Results

The mean score of CAM knowledge was 26.51, which was less than the questionnaire midpoint of 39. The mean score of attitudes toward CAM was 63.84, which was more than the questionnaire midpoint of 57. The mean score of the quality of patient care was 197.80, which was more than the questionnaire midpoint of 130. There was no significant correlation between knowledge about CAM, quality of patient care, and its dimensions. In addition, there was no significant correlation between attitudes toward CAM, quality of patient care, and its dimensions (P > 0.05).

Conclusion

The results of the present study showed that nurses' attitudes toward the use of CAM are positive and can be a sign of their readiness to use and further integrate clinically approved CAM in patient nursing care. Due to the role of nurses in patient care and treatment, there is a need to increase nurses' knowledge of CAM, and its training should be included in the nursing curriculum. More studies are needed to identify nurses' knowledge and attitudes toward CAM and its impact on the quality of nursing care.
Proton aurora and relativistic electron microbursts scattered by electromagnetic ion cyclotron waves
Authors: Shumko Mykhaylo , Gallardo-Lacourt Bea , Halford Alexa Jean , Blum Lauren W. , Liang Jun , Miyoshi Yoshizumi , Hosokawa Keisuke , Donovan Eric , Mann Ian R. , Murphy Kyle , Spanswick Emma L. , Blake J. Bernard , Looper Mark D. , Gillies D. Megan
Published: August 2022

Charged particle precipitation from Earth’s magnetosphere results in stunning displays of the aurora and energy transfer into the atmosphere. Some of this precipitation is caused by wave-particle interactions. In this study, we present an example of a wave-particle interaction between Electromagnetic Ion Cyclotron waves, and magnetospheric protons and electrons. This interaction resulted in a co-located isolated proton aurora and relativistic electron microbursts. While isolated proton aurora is widely believed to be caused by Electromagnetic Ion Cyclotron waves, this unique observation suggests that these waves can also scatter relativistic electron microbursts. Theoretically, nonlinear interactions between Electromagnetic Ion Cyclotron waves and electrons are necessary to produce the intense sub-second microburst precipitation. Lastly, detailed analysis of the auroral emissions suggests that no chorus waves were present during the event. This is in contrast to the most commonly associated driver of microbursts, whistler mode chorus waves, and supports other less commonly considered driving mechanisms.
Determinants of farmers' biosecurity mindset: A social-ecological model using systems thinking
Authors: Pao Hai-ni , Jackson Elizabeth , Yang Tsang-sung , Tsai Jyan-syung , Sung Watson H. T. , Pfeiffer Dirk U.
Published: August 2022

Commercial poultry is often farmed in high-density facilities, therefore, predisposing exposure to threats of infectious diseases. Studies suggest that it is likely that farmers have little motivation to practise on-farm biosecurity. In Taiwan, where high-density intensive poultry production is commonplace, unfortunately, several avian influenza outbreaks have occurred over the past decade despite the establishment of biosecurity procedures. To develop effective interventions, it is essential to understand the determinants of farmers' biosecurity mindset through systems thinking. In this qualitative study, we directly explored the opinions of Taiwan's chicken farmers, and a grounded theory analysis was performed. The study revealed that farmers allocate resources based on their justification for the optimisation of resource utilisation, and biosecurity is the most concerning challenge. Farmers focus on the economic aspects of their production systems, particularly when the implementation of biosecurity increases production costs, and there are multifaceted, complex barriers to implementing on-farm biosecurity. Although the participant farmers accepted to take major responsibility for disease management, paradoxically, some farmers blamed the practicality of government regulations and government employees' attitudes. Additionally, the farmers rejected the government's intentions to ask farmers to take major responsibility for the outbreaks of avian influenza while some of them intended to ignore the perceived risks. Government interventions that were considered not directly related to biosecurity also negatively influenced farmers' willingness to improve biosecurity. Using the interview results together with information in the scientific literature, we constructed a modified six-level social-ecological model to explain the complex influences of macro socio-economic conditions on farmers' biosecurity mindset. The novelty of this research lies in its wider relevance to Taiwan's chicken production industry in that it provides first-hand evidence-based knowledge to demonstrate a wide number of determinants of farmers' biosecurity mindset. This social-ecological model highlights the importance of systems thinking for the development of behavioural interventions and allows adaptation to the local context. The findings of this study have relevance to Taiwan's chicken production industry and potentially to similar systems in other countries in the wider region and should result in more effective animal health management at the farm level.
Poverty Reduction in China: Does the Agricultural Products Circulation Infrastructure Matter in Rural and Urban Areas?
Authors: Liu Xuanzhi , Zeng Fusheng
Published: August 2022

Since the reform and opening-up in 1978, a remarkable achievement has been made in poverty reduction in China. Many previous studies have analyzed the poverty reduction effects of economic growth, fiscal expenditure, education levels, information technology, urbanization, foreign trade, industrial development and other factors. As a bridge between production and consumption, the agricultural products circulation industry has an important role on the income growth of rural residents. However, the construction level of China’s agricultural products circulation infrastructure lags behind, resulting in a decline in the efficiency and quality of agricultural products supply, and farmers are also facing the embarrassing situation of increasing production without increasing income. Therefore, in this study, we selected the China’s provincial panel data from 2003 to 2017 to analyze the effect of agricultural products distribution infrastructure in narrowing the income gap between urban and rural residents and poverty reduction in China. First, we review the extant literature and elaborate the poverty reduction path of agricultural products circulation industry; secondly, we build models step by step and select nine variables; lastly, we carry out regression analysis on the overall poverty reduction effect of China’s agricultural products circulation industry, the poverty reduction effect of internal factors of agricultural products circulation industry and regional spatial heterogeneity with Stata software. Our results showed that the development level of the agricultural products circulation industry and the degree of opening-up have significant positive effects on narrowing the income gap between urban and rural residents and poverty reduction varied, with different regions in China. Among all the internal factors of development of agricultural products circulation industry in poverty reduction, the core explanatory variable is the investment in agricultural products circulation infrastructure with the largest poverty reduction effect, followed by the human capital, informatization level and number of employees of the agricultural products circulation industry, and the poverty reduction effects of the four internal development factors have had significant regional differences in China. Our findings further complement the internal mechanism of agricultural product circulation infrastructure on poverty reduction in China, suggest that we should apply appropriate strategies and give priority to the internal development factor of the agricultural product circulation industry, with the largest poverty reduction effect according to local conditions in different regions, and so be better able to achieve the targeted poverty alleviation and offer insights for the rural poverty reduction in other countries, especially in the developing countries.
Effects of Nutrients and Growth Regulators on Seed Germination and Development of Juvenile Rhizome Proliferation of Gastrodia elata In Vitro
Authors: Hsieh Chi-Hung , Liang Zeng-Chin , Shieh Wen-Jang , Chang Shin-Liang , Ho Wai-Jane
Published: August 2022

Two prevalent issues with Gastrodia elata growing in a natural wild environment are the necessary symbiotic seed germination with Mycena spp. and the long growing cycle of protocorms into mature rhizomes with Armillaria mellea. This study explored the most suitable nutrients to germinate seeds and the developmental stages and propagated protocorm/juvenile rhizomes (jrhs) through a tissue culture approach. Adding humic acid, mashed potato, peptone, and (indole-3-acetic acid) IAA to the 2 g/L Hyponex 7-6-19 basal medium accelerated seed germination into protocorms with an average length of 2 mm. The nutrients best for the in vitro propagation of jrhs consisted of 1 g/L Hyponex 7-6-19 and MS salts with 1/2 NH4NO3 and KNO3, 2 mg/L BA, 2 mg/L NAA, 2 g/L tryptone, 2 g/L glutamic acid, 1 g/L chitosan, 10 g/L apple puree, 20 g/L mashed potato, 100 mL/L coconut water, and 1.5 g/L A. mellea powder, at a pH level of 6. The maximum length and number of jrhs were 17.2 ± 0.8 mm and 76.6, respectively, in two subcultures. The in vitro propagation system started from seeds of G. elata can be employed as a potential method to propagate protocorm/jrhs for the cultivation of mature rhizomes from indoor or outdoor cultures.
The Variation of Rice Quality and Relevant Starch Structure during Long-Term Storage
Authors: Hu Hao , Li Shipeng , Pan Danjie , Wang Kaijun , Qiu Mingming , Qiu Zhuzhu , Liu Xingquan , Zhang Jiaojiao
Published: August 2022

The main substances of rice are starches, which vary their metabolism during storage. We conducted a series of tests including rice physicochemical properties, edible quality, starch content and chain length distribution along with starch structure variation to disclose the shift of rice quality by observing the changes of rice during storage. The results showed that: (1) the rice deterioration occurred as time passed, and the germination rate decreased from 70.8% to 29.4% during the storage; (2) fatty acid values increased significantly during long-term storage; (3) electrical conductivity increased as time passed; and (4) the two-year-storage rice showed significantly decreased viscosity and edible quality after sensory evaluation, decreased hardness and damaged surface area of starch granules as storage time passed. Additionally, the damaged surface area of starch granules increased with storage time. Fourier transform infrared spectroscopy (FTIR) showed that the short-range order and spiral degree of rice starch first decreased in the first year and then increased over the storage time. Furthermore, X-ray diffraction showed that the main starch of rice was A-type crystalline. Meanwhile, apparent amylose content increased from 31.00% to 33.85%, then decreased to 31.75%. The peak viscosity reduced from 2735.00 mPa·s to 2163.67 mPa·s and the disintegration value was brought down from 1377.67 mPa·s to 850.33 mPa·s. Based on the results, rice should not be stored for more than 2 years under suitable granary conditions to maintain it at a good quality.
Novel Fuller Earth, Rock Phosphate, and Biochar for Phytomanagement of Toxic Metals in Polluted Soils
Authors: Bahadur Daniyal Sher , Ahmed Samreen Riaz , Lahori Altaf Hussain , Hussain Tanveer , Alvi Sofia Khalique , Shafique Sheraz , Fatima Sadia , Vambol Viola , Mierzwa-Hersztek Monika , Hinduja Preeta , Vambol Sergij , Zhang Zengqiang
Published: August 2022

The present study was aimed to assess the efficacy of individual and combined effects of novel fuller earth, rock phosphate, and biochar (grapefruit peel) at 1% dosage on maize plant growth, soil chemical properties anduptake of toxic metals (TMs), such as Cu, Zn, Fe, and Cd, by maize plant sown in Korangi (district of Karachi, Pakistan) heavily polluted and Korangi less polluted (K-HP and K-LP) soils. The obtained results indicate that the dry biomass of maize crop increased by 14.13% with combined (FE1% + GBC1%) on K-HP soil and 18.24% with combined (FE 1% + GBC 1%) effects on K-LP soil. The maximum immobilization of Cu, Zn, Fe, and Cd was observed by 36% with GBC1%, 11.90% with FE1%, 98.97% with combined RP1% + GBC1%, 51.9% with FE1% + GBC1% for K-HP, 11.90% with FE1%, 28.6% with FE1%, 22.22% with RP1% + GBC1%, and 57.05% with FE 1% + GBC 1% for K-LP soil. After the addition of proposed substances, modification of soil OM, SOC, TOC, and pH level appeared this lead to the changes in the phyto-availability of Cu, Zn, Fe, and Cd in maize plant. It was concluded that the application of individual and combined effects of novel fuller earth, rock phosphate, and biochar (grapefruit peel) have potential to stabilize pollutants from multi-metal polluted soils for safe crop production.
Moving Buffalo Farming beyond Traditional Areas: Performances of Animals, and Quality of Mozzarella and Forages
Authors: Serrapica Francesco , Masucci Felicia , De Rosa Giuseppe , Braghieri Ada , Sarubbi Fiorella , Garofalo Francesca , Grasso Fernando , Di Francia Antonio
Published: August 2022

An observational case study was designed to highlight issues associated with a possible expansion of dairy buffalo (Bubalus bubalis) farming outside the traditional coastal plains of southern Italy. Twenty pregnant buffaloes were transferred to a hilly inland farm. After calving, production and reproduction data were collected monthly throughout lactation. From 4 to 6 months of lactation, buffaloes were enrolled in a feeding trial to evaluate the effects of locally grown forages (maize silage vs. hay) on milk production and in vivo digestibility. Sensory properties of mozzarella cheese produced at a local dairy were also evaluated. No obvious effects of diet were found. Compared to the data recorded in the previous lactation completed in the farm of origin, milk yield was reduced by 37.2%, and milk protein by 6.1%, whereas milk fat improved (+4.5%). A lower pregnancy rate (−13.3%), increased days open (+122%), and a prolonged intercalving period (+26.9%) were also observed. Lactation length was shorter than the standard value of 270 d. The results showed that peculiar reproductive characteristics, lower environmental temperatures, and the specificity of the mozzarella production process are the main problems to be addressed in an expansion of buffalo farming outside traditional areas.
The Impact of Traffic-Induced Compaction on Soil Bulk Density, Soil Stress Distribution and Key Growth Indicators of Maize in North China Plain
Authors: Wang Xianliang , He Jin , Bai Mingyue , Liu Lei , Gao Shang , Chen Kun , Zhuang Haiyang
Published: August 2022

The traffic-induced soil compaction in the field has gradually become an important constraint to sustainable agricultural development. A field experiment was conducted to acquire the stress transmitted caused by multiple passes with different types of tractors and to investigate the impact of these stresses on soil bulk density and crop growth. The experiment applied two tractors with different masses: LOVOL M904 (HC) and John Deer 280 (LC), and six different treatments of 0 (C0), 1 (C1), 3 (C3), 5 (C5), 7 (C7), and 9 (C9) tractor passes for each tractor. The results showed that at each number of passes, tractors with small axle-loads at 0–20 cm depth generated higher soil additional stress, while tractors with large axle-loads at 20–80 cm depth generated higher soil additional stress. In the 0–20 cm soil layer, when the number of continuous passes is less than 7 times, the passes of a small axle-load tractor lead to larger soil bulk density, and when the number of continuous passes is more than 7, the compaction of large axle-load tractor leads to larger soil bulk density. At depths of 20–80 cm, compaction by large axle-load tractors results in larger soil bulk density. In this study, different levels of tractor compaction inhibited key growth indicators of maize, resulting in yield reductions. The effect of different tractor load compaction on maize yield increased significantly with the number of compaction passes, with 1–5 tractor passes having no significant effect on yield and 7–9 passes producing greater yield reductions for HC. This study will provide a theoretical basis and technical support for the selection of agricultural machinery and reasonable tillage technology.
Determination of Vibration Picking Parameters of Camellia oleifera Fruit Based on Acceleration and Strain Response of Branches
Authors: Wu Delin , Zhao Enlong , Fang Dong , Jiang Shan , Wu Cheng , Wang Weiwei , Wang Rongyan
Published: August 2022

This study examines the means of reducing the damage to the branches of Camellia oleifera in the process of vibration picking and solving the problems of low equipment-development efficiency and slow product renewal caused by using traditional test methods to determine vibration picking parameters. In this study, the optimal vibration parameters were determined by using the self-response (branch acceleration and strain) law of the Camellia oleifera tree, and finite element analysis and experiments are used to solve this problem. The 3D model of Camellia oleifera was built by Solidworks. The natural frequencies of Camellia oleifera were analyzed by modal analysis, the vibration frequency and amplitude were determined by harmonic response analysis, and transient analysis was used to compare with the test results. The results show that the optimal vibration frequency range of Camellia oleifera is 4~10 Hz, and the average correlation coefficient between the maximum synthetic acceleration and the simulated value is 0.85, which shows that the model can reliably predict the vibration response. At the same time, the best vibration parameters were determined to be 9 Hz, 60 mm and 10 s. Under these parameters, the abscission rate of the Camellia oleifera fruit was 90%, and the damage rate of the flower bud was 13%. The mechanized picking effect of Camellia oleifera fruit was good. This study can quickly determine the vibration picking parameters of Camellia oleifera fruit and effectively improve the development speed of vibration picking of Camellia oleifera fruit.
Biomarker Discovery for Detecting the Seed Ageing Degree and Priming Effect of Tobacco
Authors: Niu Yongzhi , Zheng Yunye , Zhou Dongjie , Zhao Jia , Wang Chengjing , Wang Zhoufei , Zhang Limeng
Published: August 2022

Seed ageing is an important issue for the long-time seed storage of tobacco. Seed priming has been popularly applied in tobacco production. In this study, the development of molecular marker genes encoding proteins L-isoaspartyl methyltransferase NtPIMT1 and 8-oxoG DNA glycosylase 1 NtOGG1 to detect the degree of seed ageing and the effect of seed priming is conducted in tobacco. Quantitative real-time PCR (qRT-PCR) analysis reveals that relatively higher mRNAs of NtPIMT1 and NtOGG1 are observed in the dry and early germinating seeds. The expressions of NtPIMT1 and NtOGG1 are negatively correlated with the degree of seed damage in non-pelleted and pelleted seeds after accelerated ageing treatments. The early best effects of gibberellin (GA3) priming on speed and uniform germination are observed in 33 h primed seeds, and relatively lower expressions of NtPIMT1 and NtOGG1 are observed in priming seeds. NtPIMT1 and NtOGG1 genes have potential for use as molecular markers in detecting the seed ageing degree and priming effect of tobacco.
Climatic Mechanism of Delaying the Start and Advancing the End of the Growing Season of Stipa krylovii in a Semi-Arid Region from 1985–2018
Authors: Liu Erhua , Zhou Guangsheng , He Qijin , Wu Bingyi , Zhou Huailin , Gu Wenjie
Published: August 2022

Plant phenological variations depend largely on temperature, but they cannot be explained by temperature alone in arid and semi-arid regions. To reveal the response mechanisms of grassland phenology to climate change, the effects of temperature, moisture and light at the start (SOS), peak (POS) and end (EOS) of the growing season for Stipa krylovii (S. krylovii) in Inner Mongolian grassland was analysed from 1985–2018 with partial least squares (PLS) regression. The results showed that the SOS was significantly delayed at a rate of 5.4 d/10a (change over 10 years), while POS and EOS were insignificantly advanced, which were inconsistent with the existing understanding that climate warming advances the SOS and delays the EOS. The vapor pressure deficit (VPD) in July, maximum air temperature (Tmax) in September of the previous year, diurnal temperature range (DTR) from mid-February to mid-March, and Tmax from late March to mid-April of the current year were the critical factors and periods triggering the SOS, which contributed to 68.5% of the variation in the SOS. Additionally, the minimum air temperature (Tmin) occurred from mid-December to late December, and precipitation (PRE) occurred from mid-June to late July for POS, which could explain 52.1% of POS variations. In addition, Tmax from late August to early September influenced the EOS with an explanation of 49.3%. The results indicated that the phenological variations in S. krylovii were the result of the combined effects of climatic conditions from the previous year and the current year. Additionally, an increase in the preseason DTR delayed the SOS, and excessive summer precipitation induced an earlier POS, while warming in early autumn induced an earlier EOS, reflecting the adaptation mechanism of the perennial dense-cluster herbaceous plants in semi-arid regions to climate change. These findings could enrich the understanding of plant phenology in response to climate change.
Salt-Induced Autophagy and Programmed Cell Death in Wheat
Authors: Fedoreyeva Larisa I. , Lazareva Elena M. , Shelepova Olga V. , Baranova Ekaterina N. , Kononenko Neonila V.
Published: August 2022

The high salinity of soil salts limits plant growth. Wheat is sensitive to toxic levels of mineral salts. Salinity leads to the accumulation of toxic ions in all organs of wheat. Depending on the level of ion accumulation, wheat is defined as salt stress-tolerant or -sensitive. The wheat variety Zolotaya accumulated Cl− and Na+ ions to a greater extent than the Orenburgskaya 22 variety. The accumulation of toxic ions was accompanied by an increase in ROS and an increase in damage to root tissues up to 80% in the Zolotaya variety. The formation of autophagosomes is considered a defense mechanism against abiotic stresses in plants. At a concentration of 150 mM NaCl, an increase in the expression level of TOR, which is a negative regulator of the formation of autophagosomes, occurred. The level of TOR expression in the Zolotaya variety was 2.8 times higher in the roots and 3.8 times higher in the leaves than in the Orenburgskaya 22 variety. Under the action of salinity, homeostasis was disturbed in the root cells and ROS production accumulated. In the unstable variety Zolotaya, ROS was found in the cap zone and the root meristem in contrast to the resistant variety Orenburgskaya 22 in which ROS production was found only in the cap zone. Accumulation of ROS production triggered autophagy and PCD. PCD markers revealed DNA breaks in the nuclei and metaphase chromosomes, cells with a surface location of phosphatidylserine, and the release of cytochrome c into the cytoplasm, which indicates a mitochondrial pathway for the death of part of the root cells during salinity. Based on electron microscopy data, mitophagy induction was revealed in wheat root and leaf cells under saline conditions.
Estimating Leaf Nitrogen Content in Wheat Using Multimodal Features Extracted from Canopy Spectra
Authors: Gao Zhiwei , Luo Na , Yang Baohua , Zhu Yue
Published: August 2022

The leaf nitrogen content (LNC) of wheat is one of key bases for wheat nitrogen fertilizer management and nutritional diagnosis, which is of great significance to the sustainable development of precision agriculture. The canopy spectrum provides an effective way to monitor the nitrogen content of wheat. Previous studies have shown that features extracted from the canopy spectrum, such as vegetation indices (VIs) and band positions (BPs), have successfully achieved the monitoring of crop nitrogen nutrition. However, the features mentioned above are spectral features extracted on the basis of linear or nonlinear combination models with a simple structure, which limits the general applicability of the model. In addition, models based on spectral features are prone to overfitting, which also reduces the accuracy of the model. Therefore, we propose an estimation model based on multimodal features (convolutional features and VIs, BPs) of the canopy spectrum, which aim to improve accuracy in estimating wheat LNC. Among these, the convolutional features (CFs) extracted by the designed convolutional neural network represent the deep semantic information of the canopy reflection spectrum, which can make up for the lack of robustness of the spectral features. The results showed that the accuracy of the model based on the fusion features (VIs + BPs + CFs) was higher than that of the feature of single modality. Moreover, the particle swarm optimization–support vector regression (PSO-SVR) model based on multimodal features had the best prediction effect (R2 = 0.896, RMSE = 0.188 for calibration, R2 = 0.793, RMSE = 0.408 for validation). Therefore, the method proposed in this study could improve performance in the estimation of wheat LNC, which provides technical support for wheat nitrogen nutrition monitoring.
First Insights on the Karyotype Diversification of the Endemic Malagasy Leaf-Toed Geckos (Squamata: Gekkonidae: Uroplatus)
Authors: Mezzasalma Marcello , Brunelli Elvira , Odierna Gaetano , Guarino Fabio Maria
Published: August 2022

We provide here the first karyotype description of eight Uroplatus species and a characterization of their chromosomal diversity. We performed a molecular taxonomic assessment of several Uroplatus samples using the mitochondrial 12S marker and a comparative cytogenetic analysis with standard karyotyping, silver staining (Ag-NOR) and sequential C-banding + Giemsa, +Chromomycin A3 (CMA3), +4′,6-diamidino-2-phenylindole (DAPI). We found chromosomal variability in terms of chromosome number (2n = 34–38), heterochromatin composition and number and localization of loci or Nucleolar Organizer Regions (NORs) (alternatively on the 2nd, 6th, 10th or 16th pair). Chromosome morphology is almost constant, with karyotypes composed of acrocentric chromosomes, gradually decreasing in length. C-banding evidenced a general low content of heterochromatin, mostly localized on pericentromeric and telomeric regions. Centromeric bands varied among the species studied, resulting in CMA3 positive and DAPI negative or positive to both fluorochromes. We also provide evidence of a first putative heteromorphic sex chromosome system in the genus. In fact, in U. alluaudi the 10th pair was highly heteromorphic, with a metacentric, largely heterochromatic W chromosome, which was much bigger than the Z. We propose an evolutionary scenario of chromosome reduction from 2n = 38 to 2n = 34, by means of translocations of microchromosomes on larger chromosomes (often involving the NOR-bearing microchromosomes). Adding our data to those available from the literature, we show that similar processes characterized the evolutionary radiation of a larger gecko clade. Finally, we hypothesize that sex chromosome diversification occurred independently in different genera.
Competitiveness of Spanish Local Breeds
Authors: Perea José , Arias Ramón
Published: August 2022

Spain is among the countries with the greatest diversity of local breeds, as a result of an extensive and heterogeneous cultural and agroecological heritage [...]
Effect of Varying Dietary Crude Protein Level on Milk Production, Nutrient Digestibility, and Serum Metabolites by Lactating Donkeys
Authors: Yue Yuanxi , Li Li , Tong Manman , Li Shuyi , Zhao Yanli , Guo Xiaoyu , Guo Yongmei , Shi Binlin , Yan Sumei
Published: August 2022

Donkey milk is considered as a functional food due to its high levels of whey protein, and can be used in newborn nutrition, due to the nutritional similarities with human milk and its hypoallergenic property. However, donkey milk yield is very low and little is known about improving donkey milk yield by nutrition manipulation. The effect of dietary crude protein (CP) levels on milk production, nutrient digestibility, and serum metabolites was investigated in the current study. Twenty-four lactating donkeys were randomly assigned to one of the following three CP content diets: 15.3% (HP), 14.2% (MP), and 13.1% (LP) of dry matter, respectively. The experiment lasted for 10 weeks, with the first two weeks being used for adaptation. The results showed that milk yield and yields of protein, lactose, solid-not-fat, total solid, and contents of protein, total solid and milk urea nitrogen in the HP and MP groups were higher than the LP group. No significant changes were observed in dry-matter intake, contents of milk fat, lactose or solid-not-fat. The feed conversion ratio, milk protein synthesis efficiency, and the digestibility of dry matter, crude protein, ether extract, acid detergent fiber, neutral detergent fiber, calcium and phosphorous in the HP and MP groups were greater than the LP group. Serum total protein, albumin and urea nitrogen concentrations decreased, while concentrations of non-esterified fatty acids and β-hydroxybutyrate increased in the LP group compared with the HP and MP groups. In conclusion, the diet containing 14.2% CP supplied an adequate amount of protein for improving milk production in lactating donkeys, but milk production was not further increased by feeding the donkeys more than 14.2% CP.
Out of Africa: Juvenile Dispersal of Black-Shouldered Kites in the Emerging European Population
Authors: Rivera Domingo , Balbontín Javier , Pérez Gil Sergio , Abad Gómez-Pantoja José María , Negro Juan José
Published: August 2022

Knowledge of animal dispersal patterns is of great importance for the conservation and maintenance of natural populations. We here analyze juvenile dispersal of the poorly studied Black-shouldered Kite (Elanus caeruleus) monitored in southwestern Spain in an ongoing long-term study initiated in 2003. The European population of Black-shouldered kites is thought to be a recent one funded by colonizing African birds, as no kites have been found in the European fossil record, and the breeding population has progressively expanded to the North in the late 20th and 21st centuries. We obtained information on movements behavior during dispersal from 47 juveniles Kites after marking 384 nestlings with wing tags and three nestlings with radio transmitter. We have tested two competing hypotheses (i.e., the Resources Competition Hypothesis and the Wandering Hypothesis (WH)) that may explain the leptokurtic distribution of the natal dispersal distance in Elanus. After independence, juvenile females dispersed farther from the natal areas than males, as is common in birds. On average, males and females dispersed from their natal areas over 9 (i.e., 26.15 km) and 15 (i.e., 43.79 km) breeding territories, respectively. A male and two females dispersed further than 100 km from their natal nest. Our results indicated some evidence supporting the competition-for-resources hypotheses since nestlings hatched from high quality territories stayed closer from natal areas than nestlings hatched from low quality territories and also nestlings hatched first within the brood also tend to recruit closer to their natal area than later hatched nestlings which tend to disperse further away from their natal area. The information provided by these crucial demographic parameters will be used for the elaboration of future conservation plans for the management of this colonizing species in Europe.
Ovine Neosporosis: The Current Global Situation
Authors: Benavides Julio , González-Warleta Marta , Arteche-Villasol Noive , Pérez Valentín , Mezo Mercedes , Gutiérrez-Expósito Daniel
Published: August 2022

In the past 20 years, Neospora caninum infection in sheep has been reported in at least 31 countries worldwide from all sheep-rearing continents (Europe, Asia, the Americas, Africa, and Oceania), and its role as an abortifacient agent is becoming more evident. Most studies of ovine neosporosis have focused on its epidemiology, based primarily on serological analysis, with only a few studies investigating the actual presence of the parasite by PCR and/or IHC. Individual seroprevalence rates were highly variable between countries, and even between regions within the same country, ranging from 0.0% to 67.4% positive. Furthermore, most of the studies were not directly comparable due to differences in experimental designs, sample sizes, husbandry systems, ecological factors, and serological tests (e.g., IFAT, ELISA, MAT, Western blot). The latter, along with the scarcity of studies on the relevance of N. caninum as an abortifacient agent, may bias the perception of the importance of this disease. This review summarizes the situation of N. caninum infection in sheep using all available published studies describing natural ovine neosporosis. The epidemiology shows that ovine neosporosis is found worldwide, and it poses a relevant risk to the sustainability of sheep flocks.
Antifouling Performance of Carbon-Based Coatings for Marine Applications: A Systematic Review
Authors: Sousa-Cardoso Francisca , Teixeira-Santos Rita , Mergulhão Filipe J. M.
Published: August 2022

Although carbon materials are widely used in surface engineering, particularly graphene (GP) and carbon nanotubes (CNTs), the application of these nanocomposites for the development of antibiofilm marine surfaces is still poorly documented. The aim of this study was, thus, to gather and discuss the relevant literature concerning the antifouling performance of carbon-based coatings against marine micro- and macrofoulers. For this purpose, a PRISMA-oriented systematic review was conducted based on predefined criteria, which resulted in the selection of thirty studies for a qualitative synthesis. In addition, the retrieved publications were subjected to a quality assessment process based on an adapted Methodological Index for Non-Randomized Studies (MINORS) scale. In general, this review demonstrated the promising antifouling performance of these carbon nanomaterials in marine environments. Further, results from the revised studies suggested that functionalized GP- and CNTs-based marine coatings exhibited improved antifouling performance compared to these materials in pristine forms. Thanks to their high self-cleaning and enhanced antimicrobial properties, as well as durability, these functionalized composites showed outstanding results in protecting submerged surfaces from the settlement of fouling organisms in marine settings. Overall, these findings can pave the way for the development of new carbon-engineered surfaces capable of preventing marine biofouling.