bluuebunny/crossref_metadata_2025 huggingface dataset:
Suboptimal antimicrobial discharge prescriptions at a tertiary referral children’s hospital
Authors: Zhang Yi Wolf , Paturi Sruti , Puckett Lauren M. , Scheinker David , Schwenk Hayden T. , Joerger Torsten A.
Published: December 2023

Objective:

To determine the rate of and factors associated with suboptimal discharge antimicrobial prescribing at a tertiary referral children’s hospital.

Design:

Retrospective cohort.

Setting:

Tertiary referral children’s hospital.

Population:

All enteral antimicrobial discharge prescriptions at Lucile Packard Children’s Hospital Stanford from January 1st, 2021 through December 31st, 2021.

Method:

All enteral discharge antimicrobials are routinely evaluated by our antimicrobial stewardship program within 48 hours of hospital discharge. Antimicrobials are determined to be optimal or suboptimal by an antimicrobial stewardship pharmacist after evaluating the prescribed choice of antimicrobial, dose, duration, dosing frequency, and formulation. The rate and factors associated with suboptimal antimicrobial discharge prescribing were evaluated.

Results:

Of 2,593 antimicrobial prescriptions ordered at discharge, 19.7% were suboptimal. Suboptimal prescriptions were due to incorrect duration (72.2%), dose (31.0%), dose frequency (23.3%), drug choice (6.5%), or formulation (5.7%). In total, 87.2% of antimicrobials for perioperative prophylaxis and 13.5% of treatment antimicrobials were suboptimal. Antimicrobials with the highest rate of suboptimal prescriptions were amoxicillin-clavulanate (40.7%), clindamycin (36.6%), and cephalexin (36.6%).

Conclusion:

Suboptimal antimicrobial discharge prescriptions are common and present an opportunity for antimicrobial stewardship programs during hospital transition of care. Factors associated with suboptimal prescriptions differ by antimicrobial and prescribed indication, indicating that multiple stewardship interventions may be needed to improve prescribing.
Harnessing heterogeneity in behavioural research using computational social science
Authors: Veltri Giuseppe A.
Published: December 2023

Similarly to other domains of the social sciences, behavioural science has grappled with a crisis concerning the effect sizes of research findings. Different solutions have been provided to answer this challenge. This paper will discuss analytical strategies developed in the context of computational social science, namely causal tree and forest, that will benefit behavioural scientists in harnessing heterogeneity of treatment effects in RCTs. As a mixture of theoretical and data-driven approaches, these techniques are well suited to exploit the rich information provided by large studies conducted using RCTs. We discuss the characteristics of these methods and their methodological rationale and provide simulations to illustrate their use. We simulate two scenarios of RCTs-generated data and explore the heterogeneity of treatment effects using causal tree and causal forest methods. Furthermore, we outlined a potential theoretical use of these techniques to enrich behavioural science ecological validity by introducing the notion of behavioural niche.
Characterization of the West Siberian lineage of zokors (Mammalia, Rodentia, Spalacidae, Myospalacinae) and divergence in molar development
Authors: Golovanov Semion E. , Zazhigin Vladimir S.
Published: December 2023

Zokors (Myospalacinae) continue to be the center of systematics discussions. Phylogenetic schemes based on molecular data do not always agree with each other, nor can phylogenetic schemes based on paleontological material be complete due to the only-partial description of West Siberian zokors. This paper tries to fill this gap and presents a description of the West Siberian lineage from the late early Pleistocene to the present, together with an analysis of molar development in other zokor lineages. We describe two new species and one subspecies (Myospalax myospalax krukoveri n. ssp., Myospalax convexus n. sp., Prosiphneus razdoleanensis n. sp.) ancestral to the extant Myospalax myospalax Laxmann, 1769. We also reveal differences in the ontogeny of molars of modern species that were not previously detailed. These differences, together with paleontological data, indicate that in the West Siberian lineage, peramorphosis occurred in the structure of the chewing surface (with the exception of the lower m1), whereas in all other zokor lineages, there was pedomorphosis. On the basis of these results, we suggest a new view on the systematics of Myospalacinae.

UUID: http://zoobank.org/b06d6c99-1648-454b-9b95-4d869bfe8bdc
Edward Said and the Dialectic of the “Imperialized” Intellectual
Authors: Etherington Ben
Published: December 2023

This essay responds to Timothy Brennan’s recent biography of Edward Said by delving into Said’s relation to Frantz Fanon, who became an important influence in the second half of his career. Particularly, it considers whether Said’s readings and misreadings of Fanon signal a wider break with the latter’s notion of the “colonized intellectual.” Said, it emerges is more an “imperialized” intellectual, whose post-nationalist anti-imperialism is an attempt to sustain the Marxist anticolonial legacy in an era of neo-imperial consolidation. The article also considers how Said’s anti-imperialism is shaped by the idiosyncrasies and unique challenges of the Palestinian anti-colonial struggle.
New paleohydroclimate record of the MIS 5e/5d transition from Yelini Cave, central Anatolian region of Türkiye
Authors: Gürbüz Kaan , Mutlu Halim , Ünal-İmer Ezgi , Uysal İ. Tonguç , Zhao Jian-Xin
Published: December 2023

This study presents the results of a detailed paleoclimate investigation on stalagmite YL-1 (Yelini Cave, Türkiye). YL-1 grew between 117.13 (+0.57/−0.44) ka and 114.87 (+1.63/−2.89) ka within Greenland Stadial 26, indicating a positive moisture balance during the stadial conditions in this semi-arid region. Rainfall is significantly affected by sub-cloud and surface evaporation and decreasing net effective winter precipitation is recorded by high isotope values. Enriched δ18O and δ13C at 116.65 (+0.51/−0.39) ka are interpreted as a drought event that took place ca. 400 years before the end of the MIS 5e. This event, which was reported simultaneously in marine and terrestrial archives in the Northern Hemisphere, is a result of decreased cyclone activity linked to weakening of the Atlantic meridional overturning circulation. 87Sr/86Sr values of YL-1 are close to the host-rock values. Decreased 87Sr/86Sr ratio at 116.67 (+0.54/−0.38) ka reflects the intensified water–rock interaction due to lower precipitation. Along with prior calcite precipitation effect, this is also observed by increased Mg/Ca and Sr/Ca, while low P, Cu, Be, Y, and Zr concentrations indicate a lowered amount of soil-derived colloidal material. The MIS 5e/5d transition is marked by reduced insolation and enriched δ18O at 116.24 (+0.53/−0.86) ka. The Greenland Interstadial 25 phase at 115.87 (+0.83/−1.71) ka is represented by more negative δ18O and δ13C.
Political Finance and Party Systems in the Normative Theory of Partisanship: Toward a Civic Model
Authors: Bonotti Matteo , Nwokora Zim
Published: December 2023

Political finance in liberal democracies is often regarded as a source of pathology accompanied by demands for reform. But on what principles and values should political finance reform be grounded? The existing scholarship provides no more than sketchy advice on such matters. To address this gap, this paper presents a normative framework to evaluate political finance rules, which proposes (a) that the design of such rules should take account of the party system in which the financing rules will operate; (b) that both political finance rules and party systems should be evaluated in terms of three normative dimensions of partisanship (collegiality, systemic voice, and systemic accountability); and (c) that political finance reforms ought to counterbalance the pathologies inherent to different party systems. A set of political finance rules that satisfies these three conditions is an instantiation of what we describe as the ‘civic model of political finance’.
Ministerial Mandate Letters and Co-ordination in the Canadian Executive
Authors: Ie Kenny William
Published: December 2023

Prime ministers in parliamentary systems confront a challenging agency problem in leading cabinets toward cross-government priorities: ministers tend to prioritize departmental interests and may lack incentives and/or information enabling co-ordinated effort. In Canada, a novel mechanism for both increasing incentives and information provision has been developed in recent decades: the mandate letter. These letters are issued by Canadian prime ministers to their ministers, reinforcing government priorities, each minister's responsibilities, and specific policy expectations. This article examines mandate letters as mechanisms inducing interministerial policy co-ordination, focusing on the 2015–2021 period, under Justin Trudeau, as the first Canadian prime minister to release these letters publicly. Using topic modelling and social network analysis, I find that Trudeau has increasingly sought to strengthen ministerial co-ordination and ministers’ focus on crosscutting policy priorities. This case study contributes to our understanding of intraexecutive co-ordination and the agency problem in cabinet government.
Similarity and difference in large-scale functional network alternations between behavioral addictions and substance use disorder: a comparative meta-analysis
Authors: Zeng Xinglin , Han Xinyang , Zheng Dong , Jiang Ping , Yuan Zhen
Published: December 2023

Behavioral addiction (BA) and substance use disorder (SUD) share similarities and differences in clinical symptoms, cognitive functions, and behavioral attributes. However, little is known about whether and how functional networks in the human brain manifest commonalities and differences between BA and SUD. Voxel-wise meta-analyses of resting-state functional connectivity (rs-FC) were conducted in BA and SUD separately, followed by quantitative conjunction analyses to identify the common and distinct alterations across both the BA and SUD groups. A total of 92 datasets with 2444 addicted patients and 2712 healthy controls (HCs) were eligible for the meta-analysis. Our findings demonstrated that BA and SUD exhibited common alterations in rs-FC between frontoparietal network (FPN) and other high-level neurocognitive networks (i.e. default mode network (DMN), affective network (AN), and salience network (SN)) as well as hyperconnectivity between SN seeds and the Rolandic operculum in SSN. In addition, compared with BA, SUD exhibited several distinct within- and between-network rs-FC alterations mainly involved in the DMN and FPN. Further, altered within- and between-network rs-FC showed significant association with clinical characteristics such as the severity of addiction in BA and duration of substance usage in SUD. The common rs-FC alterations in BA and SUD exhibited the relationship with consistent aberrant behaviors in both addiction groups, such as impaired inhibition control and salience attribution. By contrast, the distinct rs-FC alterations might suggest specific substance effects on the brain neural transmitter systems in SUD.
Exploiting the deep seabed for the benefit of humankind: A universal ideology for sustainable resource development or a false necessity?
Authors: Roland Holst Rozemarijn J.
Published: December 2023

A pivotal point in time has been reached in the ongoing negotiations under the auspices of the International Seabed Authority (ISA) towards the adoption of regulations for the commercial exploitation of mineral resources in the deep seabed beyond national jurisdiction. The ISA has a mandate to ensure that activities in the Area, legally designated as ‘common heritage of humankind’, are carried out for the benefit of humankind as a whole. Yet, there is a growing sense of unease with the potential imminence of the commercial exploitation phase, and concern that the implementation of all components of the common heritage principle, including its environmental and distributive ambitions, will be compromised in the interest of a handful of industry stakeholders. This article dives under the surface of these tensions by asking how the public interest in a global commons can become constructed in a way that conflates diverse and opposing interests in favour of value extraction by the private sector, revealing the ambivalent role of international law in the process. It uses the concept of ‘false necessity’ to question the apparent urgency and inevitability of commercial exploitation, more specifically to the extent it obscures and pre-empts more inclusive conceptions of ‘benefit’ for humankind. By shifting the focus from the much-debated risks of deep seabed mining to the notion of benefit, the article illuminates the inherent contradictions and distributional asymmetries obscured by the conflated yet purportedly universal conception of public interest in exploitation.
The ‘ideal victim’: A cage for victims’ narratives at the International Criminal Court
Authors: Cuppini Alessandra
Published: December 2023

Despite Article 68(3) of the Rome Statute of the International Criminal Court (ICC) granting victims an autonomous standing in proceedings, victims’ participatory rights have often been tailored to fit within the retributive structure of the trials. This contribution aims to provide a different perspective on victims’ role and their narratives in proceedings at the ICC, building upon the expressivist model of international criminal justice and focusing on a specific strand that engages with the adjudication process’s performative and communicative features. In providing a better understanding of how victims’ narrative unfolds in trials at the ICC, the article addresses two issues: how the concept of the victim is constructed at the ICC; and whether and, eventually, how this construct impedes progress in recognizing their narratives in proceedings at the ICC. Concerning the first issue, drawing on criminologist Nils Christie’s theorizing of the ‘ideal victim’, it will be observed that the construct of victims in proceedings at the ICC reflects three main attributes: weakness; innocence; and dependency. The second issue shed light on the extent to which the emphasis on the ‘ideal victim’ can serve as a tool in the hands of institutional actors at the ICC to pre-empt, constrain and subordinate victims’ narratives, in a manner that oversimplifies victimhood. To impose a particular narrative upon victims’ experiences, three main procedural mechanisms have been identified: appropriation of victims’ interests; legal representation of abstract victimhood; and exclusion from the trial of victims who do not conform to the ideal victim.
Cytoplasmic granules in bovine oocytes do not affect embryonic or fetal development
Authors: Rosa Paola Maria da Silva , Guedes Pedro Henrique Evagelista , Garcia Joaquim Mansano , Oliveira Clara Slade
Published: December 2023

Oocyte cytoplasmic evaluation is based on homogeneity and granular appearance. Our study investigated if a granular cytoplasm, highly heterogeneous, would affect oocyte competence in bovine. In two experiments, bovine cumulus–oocyte complexes (COCs) with homogeneous cytoplasm (control, CC) and granulated cytoplasm (granular, GC) were selected from a regular pool of COCs. Experiment 1 was performed with slaughterhouse ovaries, and Experiment 2 was carried out in Girolando COCs obtained from ovum pick-up. Granular oocytes had higher caspase 3 levels (66.17 ± 11.61 vs 172.08 ± 16.95, P < 0.01) and similar GAP junction activity (5.64 ± 0.45 vs 6.29 ± 0.29). ZAR1 relative mRNA amount was lower in granular oocytes (178.27 ± 151.63 vs 0.89 ± 0.89, P = 0.01) and no effect was detected for MATER, PPP2R1A, ENY2, IGF2R, and BMP15 genes. Despite molecular differences, no detrimental effect was detected on oocyte competence in GC oocytes. Cleavage (Experiment 1: 59.52 ± 7.21% vs 59.79 ± 6.10% and Experiment 2: 68.88 ± 4.82 vs 74.41 ± 5.89%) and blastocyst (Experiment 1: 29.28 ± 4.14% vs 23.15 ± 2.96% and Experiment 2: 21.11 ± 3.28% vs 21.02 ± 6.08%) rates were similar between CC and GC (Experiments 1 and 2, respectively). Post-transfer embryo development revealed that pregnancy (CC: 24.27 ± 9.70% vs GC: 26.31 ± 7.23%) and calving (23.68% vs 33.33%) rates and fetal growth were not affected by the presence of cytoplasmic granules. Our results demonstrated that oocytes with granular cytoplasm present equivalent efficiency for IVF and calf production compared with homogenous cytoplasm oocytes. This could be observed through similar cleavage, blastocyst rates, and fetal growth development. In addition to differences in oocyte gene expression related to oocyte quality, it seems not to affect oocyte developmental competence.
House prices and rents: a reappraisal
Authors: Achou Bertrand , d’Albis Hippolyte , Iliopulos Eleni
Published: December 2023

In this work, we introduce rental markets in a general equilibrium model with borrowing constraints and infinitely lived agents. We estimate our model using standard Bayesian methods and match US data on recent decades. We highlight a crucial relationship that strongly links interest rates, house prices, and rents. It represents agents’ arbitrage when choosing their degree of participation in the housing market (i.e. their real estate holdings). This framework is particularly well suited for explaining how policy-induced changes in households’ preferences have driven house prices up while pushing rent-price ratios down in the aftermath of the Covid-19 outbreak. It also allows us to parsimoniously track the unequal impact of these changes on agents’ decisions and welfare, which crucially depends on whether they are owners or renters.
The ingroup love and outgroup hate of Christian Nationalism: experimental evidence about the implementation of the rule of law
Authors: Broeren Zachary D. , Djupe Paul A.
Published: December 2023

A long line of research has established that Americans who subscribe to Christian nationalism have a preference for those inside their group and animosity toward those outside their group. These beliefs may impede the equal application of the rule of law, a link that has been suggested but not formally tested. Utilizing experimental data from a survey conducted in fall 2021, we assess the equal application of the rule of law for in and outgroup members conditional on Christian nationalism and belief in Christian persecution. We suggest that ingroup love may move distinctly from outgroup hate. Our results suggest that Christian nationalists have a preference for the ingroup, but do not automatically denigrate outgroups. However, belief in Christian persecution drives animosity toward outgroups, while not elevating the ingroup. Christian nationalist outgroup hatred must be triggered by threat, which has been the project of movement and party elites.
Associations between epigenome-wide DNA methylation and height-related traits among Sub-Saharan Africans: the RODAM study
Authors: Swart Galatea , Meeks Karlijn , Chilunga Felix , Venema Andrea , Agyemang Charles , van der Linden Eva , Henneman Peter
Published: December 2023

Human height and related traits are highly complex, and extensively research has shown that these traits are determined by both genetic and environmental factors. Such factors may partially affect these traits through epigenetic programing. Epigenetic programing is dynamic and plays an important role in controlling gene expression and cell differentiation during (early) development. DNA methylation (DNAm) is the most commonly studied epigenetic feature. In this study we conducted an epigenome-wide DNAm association analysis on height-related traits in a Sub-Saharan African population, in order to detect DNAm biomarkers across four height-related traits. DNAm profiles were acquired in whole blood samples of 704 Ghanaians, sourced from the Research on Obesity and Diabetes among African Migrants study, using the Illumina Infinium HumanMethylation450 BeadChip. Linear models were fitted to detect differentially methylated positions (DMPs) and regions (DMRs) associated with height, leg-to-height ratio (LHR), leg length, and sitting height. No epigenome-wide significant DMPs were recorded. However we did observe among our top DMPs five informative probes associated with the height-related traits: cg26905768 (leg length), cg13268132 (leg length), cg19776793 (height), cg23072383 (LHR), and cg24625894 (sitting height). All five DMPs are annotated to genes whose functions were linked to bone cell regulation and development. DMR analysis identified overlapping DMRs within the gene body of HLA-DPB1 gene, and the HOXA gene cluster. In this first epigenome-wide association studies of these traits, our findings suggest DNAm associations with height-related heights, and might influence development and maintenance of these traits. Further studies are needed to replicate our findings, and to elucidate the molecular mechanism underlying human height-related traits.
Dear NSF: Scientific Ocean Drilling Is Necessary for Climate Studies, Natural Hazards Research, and Inspiring Future Scientists
Authors: Hoegler Brianna , Nirenberg Jared
Published: December 2023

This letter, written by two early career researchers, highlights their urgent concerns regarding the future of scientific ocean drilling (SciOD) research following the retirement of the JOIDES Resolution (JR) at the end of the 2024 fiscal year. In the letter, addressed to the director of NSF‐OCE and to key leaders in determining U.S. scientific priorities, we outline the importance of scientific ocean drilling, both historically and for future scientific advances. We urge the NSF to make immediate, concrete commitments to fund, develop, and construct a new riserless drilling vessel dedicated to SciOD. We also highlight the importance of supporting existing and future SciOD researchers and infrastructure, including the urgent need to create programs designed to support early career researchers in our field. The letter has been signed by nearly three hundred scientists, more than two hundred of which are U.S.‐based researchers and more than half of which are early career researchers.
Long Exposure Chandra X‐Ray Observation of Jupiter's Auroral Emissions During Juno Plasmasheet Encounters in September 2021
Authors: McEntee S. C. , Jackman C. M. , Weigt D. M. , Louis C. K. , Dunn W. R. , Boudouma A. , Connerney J. E. P. , Kurth W. S. , Kraft R. , Branduardi‐Raymont G. , Gladstone G. R. , Rutala M. J.
Published: December 2023

On 15 September 2021, Chandra carried out a 40‐hr (∼4 jovian rotations) observation as part of its longest planetary campaign to study the drivers of jovian X‐ray aurora that may be linked to ultra‐low frequency (ULF) wave activity. During this time, Juno's orbit had taken the spacecraft into Jupiter's dusk magnetosphere. Here is believed to be the most probable location of ULF waves propagating along jovian magnetic field lines that drive the X‐ray auroral emissions. This is the first time that this region has been observed by an orbiter since Galileo >20 years ago, and never before has there been contemporaneous in situ and X‐ray observations. A 1D solar wind propagation model identifies a compression event near the midpoint of the 40‐hr observation window. The influence of a compression is confirmed when comparing the measured magnetic field in the dusk lobes of the magnetotail from Juno MAG data against a baseline lobe field model. Data from the Juno Waves instrument also show activation of broadband kilometric (bKOM) emissions during the arrival of the shock, a feature that has previously been observed during compression events. Therefore this is the first time we can fully analyze the morphological variability during the evolution of a shock. Wavelet transforms and Rayleigh testing are used to search for statistically significant quasi‐periodic pulsations (QPPs) of the X‐ray emissions in the data set, and find significant QPPs with periods of 25–26 min for the northern auroral X‐rays.
The Evolution of Solar Tide‐Like Signatures in the Ionospheric Total Electron Content During Major Sudden Stratospheric Warming Events
Authors: Ma Han , Liu Libo , He Maosheng , Yu You , Zhang Ruilong , Lyu Haixia , Li Wenbo , Chen Yiding , Le Huijun
Published: December 2023

By using total electron content (TEC) from the global ionospheric maps, the solar tide‐like signatures in the ionosphere are explored. We further study how the main components evolve during the 2009 and 2018 major stratospheric sudden warming events (SSWs). The method of a least‐square fit is applied for extracting the solar tide‐like components. The three predominant components are SW2, D0, and SW1, amplitude of which increase in the equatorial ionization anomaly region after the onset of both SSW events, especially in the northern hemisphere. During the SSW events, the solar tide‐like components vary significantly with latitude and their evolutions show hemispherical asymmetries. Each same component presents similar evolutionary trend during both events. The characteristics of ionospheric tide‐like signatures and related mechanisms are discussed. The SW2 tide‐like responses in TEC closely follow the changes in the tidal wind fields in the lower thermosphere, which provide evidences of the atmosphere‐ionosphere coupling during SSW events. In addition, the latitudinal asymmetric distributing of stratospheric ozone and ionospheric TEC background during the SSW may be influence factors for the hemispherical asymmetries in tide‐like signatures responses in TEC.
Defining the Multiscalar Index Timescale—Soil Water Depth Continuum for the Southwestern United States
Authors: McKellar Trevor T. , Crimmins Michael A. , Schaap Marcel G. , Rasmussen Craig
Published: December 2023

The lack of long‐term reliable in‐situ soil moisture data sets creates unique drought monitoring challenges for the semi‐arid Southwestern United States (“Southwest”). Land managers use multiscalar meteorological drought indices, like the Standardized Precipitation Index (SPI) and Standardized Precipitation‐Evapotranspiration Index (SPEI), as proxies for soil water availability; however, objectively identifying the index and timescale that best represents soil water availability in semi‐arid environments remains a significant gap for applying available climate information to land management action. Here, we couple site‐specific soil modeling with high resolution, spatially continuous meteorological data sets to define the relationship between index timescale length and soil water availability of different depths. By creating new matric potential indices (MPI) from 0 to 200 cm, we correlate MPI time series with the SPI and SPEI at different timescale lengths to identify the best index‐timescale combination for each depth. Results indicate the general relationship between highest correlating index‐timescale and MPI‐depth operates roughly on a 1‐month:5 cm step progression at shallow depths (<80 cm). Analysis by soil texture class shows that soils with higher clay content produce shallower sloped relationships (>1‐month:5 cm) than sandy soils (<1‐month:5 cm). Overall, the SPI produced higher correlations and less error with the MPI compared to the SPEI across all texture classes and depths. Thus, the SPI is a good indicator of soil water availability at shallow depths (<80 cm) and should be considered for soil drought monitoring on Southwestern drylands. However, land managers should consult local soils information, if available, given the impacts of texture class on timescale‐depth relationships.
Methane Gas Ebullition Dynamics From Different Subtropical Wetland Vegetation Communities in Big Cypress National Preserve, Florida Are Revealed Using a Multi‐Method, Multi‐Scale Approach
Authors: Sirianni Matthew J. , Comas Xavier , Anderson Frank E.
Published: December 2023

Methane (CH4) emissions from peat soils are highly variable in space and time and are influenced by changes in biogeochemical controls and other environmental factors. Areas or times with disproportionally high CH4 emissions in wetlands may develop where conditions are especially conducive for microbial processes like methanogenesis. Currently, eddy covariance methods are employed to quantify CH4 exchanges over several extensive subtropical forested wetland communities in the Big Cypress National Preserve, Florida. In this work, we investigate the importance of multi‐scale measurements to characterize CH4 ebullition dynamics from subtropical wetlands. Our approach uses a combination of gas traps, time‐lapse photography, and capacitance probes to characterize ebullition dynamics from two different wetland vegetation communities for comparison to eddy covariance CH4 measurements at the site. Ground‐penetrating radar surveys and soil sampling are used to assess differences in subsurface properties between sites that influence ebullition. Our results show that the mean measurement bias between fluxes measured in this study and the eddy covariance measurements over the same period was 10–14 times larger during the wet season when ebullition rates were greatest, than the dry season, when ebullition rates were smallest. This suggests that eddy covariance measurements may underestimate the CH4 contribution of ebullition across heterogeneous wetland vegetation communities and that the comparability of CH4 fluxes from methods varying in spatio‐temporal scale changes in response to subtropical Florida seasonality. Our work suggests that these methods can be used to complement eddy covariance measurements and improve the characterization of ebullition dynamics in subtropical wetlands.
A Multivariate Frequency Analysis Framework to Estimate the Return Period of Hurricane Events Using Event‐Based Copula
Authors: Cho Eunsaem , Ahmadisharaf Ebrahim , Done James , Yoo Chulsang
Published: December 2023

This study proposed a framework to evaluate multivariate return periods of hurricanes using event‐based frequency analysis techniques. The applicability of the proposed framework was demonstrated using point‐based and spatial analyses on a recent catastrophic event, Hurricane Ian. Univariate, bivariate, and trivariate frequency analyses were performed by applying generalized extreme value distribution and copula on annual maximum series of flood volume, peak discharge, total rainfall depth, maximum wind speed, wave height and storm surge. As a result of point‐based analyses, return periods of Hurricane Ian was investigated by using our framework; univariate return periods were estimated from 39.2 to 60.2 years, bivariate from 824.1 to 1,592.6 years, and trivariate from 332.1 to 1,722.9 years for the Daytona‐St. Augustine Basin. In the Florida Bay‐Florida Keys Basin, univariate return periods were calculated from 7.5 to 32.9 years, bivariate from 36.5 to 114.9 years, and trivariate from 25.0 to 214.8 years. Using the spatial analyses, we were able to generate the return period map of Hurricane Ian across Florida. Based on bivariate frequency analyses, 18% of hydrologic unit code 8 (HUC8) basins had an average return period of more than 30 years. Sources of uncertainty, due to the scarcity of analysis data, stationarity assumption and impact of other weather systems such as strong frontal passages, were also discussed. Despite these limitations, our framework and results will be valuable in disaster response and recovery.
Pediatric produce prescription initiatives in the U.S.: a scoping review
Authors: Muleta Hemen , Fischer Laura K. , Chang Megan , Kim Noah , Leung Cindy W. , Obudulu Chinwe , Essel Kofi
Published: December 2023

Background

To describe pediatric Produce Prescription (PRx) interventions and their study designs, outcomes, and opportunities for future research.

Methods

A scoping review framework was used to describe PRx interventions published between January 2000 and September 2023. Articles from online databases were uploaded into Covidence. Data on study characteristics, outcomes of interest (health, food insecurity (FI), nutritional and culinary efficacy, and fruit and vegetable (F/V) consumption), and feasibility were extracted. The Mixed Methods Appraisal Tool (MMAT) was used for quality assessment.

Results

19 articles met inclusion criteria. Ten studies were quantitative, five were qualitative, and four used mixed-methods. Interventions included food vouchers (n = 14) or food box/pantries (n = 5). Four studies allowed food items in addition to F/Vs. Six studies measured changes in FI and five reported a statistically significant decrease. Seven studies measured changes in F/V consumption and five reported a statistically significant increase. One study reported a statistically significant reduction in child BMI z-score. Most studies reported high feasibility. Few studies used high-quality methods.

Conclusions

Pediatric PRx interventions show promising potential to reduce FI and improve diet quality and health-related outcomes. Future studies should utilize rigorous study designs and validated assessment tools to understand the impact of pediatric PRx on health.

Impact

This work offers a summary of programmatic outcomes including retention, redemption, incentives, nutrition education, study design and quality limitations to help inform future work.

We found positive impacts of pediatric produce prescriptions (PRx) on FI, F/V consumption, and nutritional knowledge and culinary skills.

More high-quality, rigorous studies are needed to understand the best delivery and design of PRx and their impact on child behavior and health outcomes.

This work provides support for the need for rigorous studies and the potential for PRx to play a role in multi-pronged strategies that address pediatric FI and diet-related disease.
Dermal features derived from optoacoustic tomograms via machine learning correlate microangiopathy phenotypes with diabetes stage
Authors: Karlas Angelos , Katsouli Nikoletta , Fasoula Nikolina-Alexia , Bariotakis Michail , Chlis Nikolaos-Kosmas , Omar Murad , He Hailong , Iakovakis Dimitrios , Schäffer Christoph , Kallmayer Michael , Füchtenbusch Martin , Ziegler Annette , Eckstein Hans-Henning , Hadjileontiadis Leontios , Ntziachristos Vasilis
Published: December 2023

Skin microangiopathy has been associated with diabetes. Here we show that skin-microangiopathy phenotypes in humans can be correlated with diabetes stage via morphophysiological cutaneous features extracted from raster-scan optoacoustic mesoscopy (RSOM) images of skin on the leg. We obtained 199 RSOM images from 115 participants (40 healthy and 75 with diabetes), and used machine learning to segment skin layers and microvasculature to identify clinically explainable features pertaining to different depths and scales of detail that provided the highest predictive power. Features in the dermal layer at the scale of detail of 0.1–1 mm (such as the number of junction-to-junction branches) were highly sensitive to diabetes stage. A ‘microangiopathy score’ compiling the 32 most-relevant features predicted the presence of diabetes with an area under the receiver operating characteristic curve of 0.84. The analysis of morphophysiological cutaneous features via RSOM may allow for the discovery of diabetes biomarkers in the skin and for the monitoring of diabetes status.
Decoupling speciation and extinction reveals both abiotic and biotic drivers shaped 250 million years of diversity in crocodile-line archosaurs
Authors: Payne Alexander R. D. , Mannion Philip D. , Lloyd Graeme T. , Davis Katie E.
Published: December 2023

Whereas living representatives of Pseudosuchia, crocodylians, number fewer than 30 species, more than 700 pseudosuchian species are known from their 250-million-year fossil record, displaying far greater ecomorphological diversity than their extant counterparts. With a new time-calibrated tree of >500 species, we use a phylogenetic framework to reveal that pseudosuchian evolutionary history and diversification dynamics were directly shaped by the interplay of abiotic and biotic processes over hundreds of millions of years, supported by information theory analyses. Speciation, but not extinction, is correlated with higher temperatures in terrestrial and marine lineages, with high sea level associated with heightened extinction in non-marine taxa. Low lineage diversity and increased speciation in non-marine species is consistent with opportunities for niche-filling, whereas increased competition may have led to elevated extinction rates. In marine lineages, competition via increased lineage diversity appears to have driven both speciation and extinction. Decoupling speciation and extinction, in combination with ecological partitioning, reveals a more complex picture of pseudosuchian evolution than previously understood. As the number of species threatened with extinction by anthropogenic climate change continues to rise, the fossil record provides a unique window into the drivers that led to clade success and those that may ultimately lead to extinction.
Modeling fragment counts improves single-cell ATAC-seq analysis
Authors: Martens Laura D. , Fischer David S. , Yépez Vicente A. , Theis Fabian J. , Gagneur Julien
Published: December 2023

Single-cell ATAC sequencing coverage in regulatory regions is typically binarized as an indicator of open chromatin. Here we show that binarization is an unnecessary step that neither improves goodness of fit, clustering, cell type identification nor batch integration. Fragment counts, but not read counts, should instead be modeled, which preserves quantitative regulatory information. These results have immediate implications for single-cell ATAC sequencing analysis.
The association between homocysteine and bacterial vaginosis: results from NHANES 2001–2004
Authors: Luo Jing , Chen Tong , Chen Yue , Huang Ze-Min , Li Xiu-Juan , Chen Hao-Kai , Huang Yi-Qi , Guo Xu-Guang
Published: December 2023

Although no study has directly shown the relationship between bacterial vaginosis (BV) and homocysteine (HCY), we still found some association between these two through extensive literature and data analysis. BV score was calculated by Nugent’s method, less than equal to 6 is negative and greater than equal to 7 is positive. This article describes interrelationships we mined from data extracted by NHANES regarding BV and HCY under multiple covariates. We used two cycles of NHANES 2001–2002 and 2003–2004 in our study. We included 2398 participants in our study who recently completed the interview and the MEC tests. By investigating the relationship between BV and HCY under multivariate conditions, multiple linear regression analysis was performed. These factors may have influenced the results, such as ethnicity, age, education level, body mass index (BMI), etc. Serum vitamin B12, ferritin, percentage of segmented centrioles, and number of segmented centrioles were selected as potential covariates in our study. We observed that both the coarse model and the two adjusted models showed a high correlation between HCY and BV, and the correlation was positive. In the coarse model, OR = 1.26, 95% confidence interval (CI) 1.10, 1.44, P = 0.0018); HCY was positively correlated with BV (OR = 1.19, 95% confidence interval (CI) 1.05, 1.34, P = 0.0121). Multiple linear regression analysis was used to investigate the connection between BV and HCY under multivariate settings. The results of this study indicate that HCY is positively associated with the prevalence of BV and may play an important role in the prevention and management of BV.
Impact of chitosan-incorporated toothpaste on roughness, gloss, and antifungal potential of acrylic resin
Authors: Varaschin Theodorovicz Kaye , Vieira-Junior Waldemir Franscisco , Manoel Garcia Raissa , Pini Simões Gobbi Ludmila , Mayume Mori Mariana , Prado Dias Filho Benedito , Alves Nunes Leite Lima Débora , Sundfeld Daniel , Pavesi Pini Núbia Inocencya
Published: December 2023

This study aimed to test the efficacy of different silica-based toothpastes with or without chitosan, as a method of cleaning the acrylic surfaces of denture prostheses. Acrylic resin specimens were prepared to evaluate surface roughness and gloss (n = 10), and Candida albicans adhesion/inhibition (n = 2). Two toothpastes with different degrees of abrasiveness were used: Colgate (CT) and Elmex (EX), with or without 0.5% chitosan (Ch) microparticles (CTCh or EXCh, respectively). The negative control was brushed with distilled water. Brushing was simulated with a machine. Surface roughness and gloss were analyzed before and after brushing. Candida albicans incidence/inhibition was tested qualitatively to determine the acrylic resin antifungal activity. The roughness and gloss data were analyzed with a generalized linear model, and the Kruskal Wallis and Dunn tests, respectively (α = 5%). Brushing with toothpastes increased roughness and reduced gloss, compared with the negative control (p < 0.05). CT showed a more significantly different change in roughness and gloss, in relation to the other groups (p < 0.05). Addition of chitosan to CT reduced its abrasive potential, and yielded results similar to those of EX and EXCh. Specimens brushed with CT showed a higher potential for Candida albicans adherence, despite its higher antifungal action. Addition of chitosan to the toothpaste made both toothpaste and brushing more effective in inhibiting Candida albicans. CT had the potential to increase roughness, reduce gloss, and increase Candida albicans adherence. In contrast, chitosan added to CT showed greater antifungal potential, and a higher synergistic effect than EX.
Periostin facilitates ovarian cancer recurrence by enhancing cancer stemness
Authors: Huang Zhiqing , Byrd Olivia , Tan Sarah , Hu Katrina , Knight Bailey , Lo Gaomong , Taylor Lila , Wu Yuan , Berchuck Andrew , Murphy Susan K.
Published: December 2023

The lethality of epithelial ovarian cancer (OC) is largely due to a high rate of recurrence and development of chemoresistance, which requires synergy between cancer cells and the tumor microenvironment (TME) and is thought to involve cancer stem cells. Our analysis of gene expression microarray data from paired primary and recurrent OC tissues revealed significantly elevated expression of the gene encoding periostin (POSTN) in recurrent OC compared to matched primary tumors (p = 0.015). Secreted POSTN plays a role in the extracellular matrix, facilitating epithelial cell migration and tissue regeneration. We therefore examined how elevated extracellular POSTN, as we found is present in recurrent OC, impacts OC cell functions and phenotypes, including stemness. OC cells cultured with conditioned media with high levels of periostin (CMPOSTNhigh) exhibited faster migration (p = 0.0044), enhanced invasiveness (p = 0.006), increased chemoresistance (p < 0.05), and decreased apoptosis as compared to the same cells cultured with control medium (CMCTL). Further, CMPOSTNhigh-cultured OC cells exhibited an elevated stem cell side population (p = 0.027) along with increased expression of cancer stem cell marker CD133 relative to CMCTL-cultured cells. POSTN-transfected 3T3-L1 cells that were used to generate CMPOSTNhigh had visibly enhanced intracellular and extracellular lipids, which was also linked to increased OC cell expression of fatty acid synthetase (FASN) that functions as a central regulator of lipid metabolism and plays a critical role in the growth and survival of tumors. Additionally, POSTN functions in the TME were linked to AKT pathway activities. The mean tumor volume in mice injected with CMPOSTNhigh-cultured OC cells was larger than that in mice injected with CMCTL-cultured OC cells (p = 0.0023). Taken together, these results show that elevated POSTN in the extracellular environment leads to more aggressive OC cell behavior and an increase in cancer stemness, suggesting that increased levels of stromal POSTN during OC recurrence contribute to more rapid disease progression and may be a novel therapeutic target. Furthermore, they also demonstrate the utility of having matched primary-recurrent OC tissues for analysis and support the need for better understanding of the molecular changes that occur with OC recurrence to develop ways to undermine those processes.
Protective effects of niacin following high fat rich diet: an in-vivo and in-silico study
Authors: Samad Noreen , Manzoor Natasha , Batool Ammara , Noor Aqsa , Khaliq Saima , Aurangzeb Sana , Bhatti Sheraz Ahmed , Imran Imran
Published: December 2023

Niacin had long been understood as an antioxidant. There were reports that high fat diet (HFD) may cause psychological and physical impairments. The present study was aimed to experience the effect of Niacin on % growth rate, cumulative food intake, motor activity and anxiety profile, redox status, 5-HT metabolism and brain histopathology in rats. Rats were administered with Niacin at a dose of 50 mg/ml/kg body weight for 4 weeks following normal diet (ND) and HFD. Behavioral tests were performed after 4 weeks. Animals were sacrificed to collect brain samples. Biochemical, neurochemical and histopathological studies were performed. HFD increased food intake and body weight. The exploratory activity was reduced and anxiety like behavior was observed in HFD treated animals. Activity of antioxidant enzymes was decreased while oxidative stress marker and serotonin metabolism in the brain of rat were increased in HFD treated animals than ND fed rats. Morphology of the brain was also altered by HFD administration. Conversely, Niacin treated animals decreased food intake and % growth rate, increased exploratory activity, produced anxiolytic effects, decreased oxidative stress and increased antioxidant enzyme and 5-HT levels following HFD. Morphology of brain is also normalized by the treatment of Niacin following HFD. In-silico studies showed that Niacin has a potential binding affinity with degradative enzyme of 5-HT i.e. monoamine oxidase (MAO) A and B with an energy of ~ − 4.5 and − 5.0 kcal/mol respectively. In conclusion, the present study showed that Niacin enhanced motor activity, produced anxiolytic effect, and reduced oxidative stress, appetite, growth rate, increased antioxidant enzymes and normalized serotonin system and brain morphology following HFD intake. In-silico studies suggested that increase 5-HT was associated with the binding of MAO with Niacin subsequentially an inhibition of the degradation of monoamine. It is suggested that Niacin has a great antioxidant potential and could be a good therapy for the treatment of HFD induced obesity.
Identification and validation of a T cell marker gene-based signature to predict prognosis and immunotherapy response in gastric cancer
Authors: Zhong Jinlin , Pan Rongling , Gao Miao , Mo Yuqian , Peng Xin , Liang Guoxiao , Chen Zixuan , Du Jinlin , Huang Zhigang
Published: December 2023

Although the role of T cells in tumor immunity and modulation of the tumor microenvironment (TME) has been extensively studied, their precise involvement in gastric adenocarcinoma remains inadequately explored. In this work, we analyzed the single-cell RNA sequencing data set in GSE183904 and identified 322 T cell marker genes using the “FindAllMarkers” method of the R package “Seurat”. STAD patients in the TCGA database were divided into high-risk and low-risk categories based on risk scores. The five-gene prediction signature based on T cell marker genes can predict the prognosis of gastric cancer patients with high accuracy. In the training cohort, the areas under the receiver operating characteristic (ROC) curve were 0.667, 0.73, and 0.818 at 1, 3, and 5 years. External validation of the predictive signature was also performed using multiple clinical subgroups and GEO cohorts. To help with practical application, a diagnostic model was created that shows values of 0.732, 0.752, and 0.816 for the relevant areas under the ROC curve at 1, 3, and 5 years. The T cell marker genes identified in this study may serve as potential therapeutic targets, and the developed predictive signatures and nomograms may aid in the clinical management of gastric cancer.