bluuebunny/crossref_metadata_2025 huggingface dataset:
КОГНИТИВНАЯ МОДЕЛЬ КОЭВОЛЮЦИИ БИОСФЕРЫ И ЧЕЛОВЕЧЕСКОГО ОБЩЕСТВА
Authors: Меншуткин В.В. , Левченко В.Ф.
Published: April 2019

Разработана простая когнитивная модель антропосферы, включающая в себя характеристики природных экосистем, человеческих популяций, промышленного производства, загрязнения среды и расходования ископаемых ресурсов. Поверхность Земли представлена в виде 20 ячеек (регионов), соединенных миграционными и информационными связями. Исследовано влияние различных стратегий природопользования на динамику всей системы, а также чувствительность системы к колебаниям климата и изменению внутренних параметров. Критерием оптимального управления выбран уровень жизни населения. Сделан вывод о необходимости включения «человеческого фактора» в модели глобального развития.
Ukrainian-Georgian Relations at the present Stage
Authors: Zyabkina E. I.
Published: April 2019

After the collapse of the USSR Ukraine and Georgia began to develop their interstate relations actively. This development was facilitated by the political processes in each of the states, as well as the foreign policy pursued by Kiev and Tbilisi. Initially, countries focused on expanding interaction with Western countries, which were considered to be potential partners. Ukraine and Georgia were eager to develop the relations with the EU and NATO. In turn, European countries and the United States paid increased attention to Ukraine and Georgia.

The alignment of forces in each of the states contributed to the rapprochement of Kiev and Tbilisi. The political forces of both countries pursued a course of distancing from Russia and rapprochement with Western countries. As a result, Ukrainian-Georgian relations relied on similar political positions, which allowed countries to expand cooperation on a bilateral basis and within the framework of multilateral cooperation mechanisms.

In recent years Ukrainian-Georgian relations have been developing dynamically. Two states are pursuing a policy of joining NATO, expanding cooperation with the EU. Ukraine is interested in deepening cooperation with Tbilisi due to strained relations with Russia. In addition, Ukraine seeks to use the Western sanctions policy towards Russia to solve domestic political and socio-economic problems. Whereas, Western countries are interested in strengthening the Ukrainian-Georgian tandem hoping to use it in promotion of their interests in the post-Soviet space and in implementing policies towards Russia.

This article explores the prospects for the development of interstate Ukrainian-Georgian relations, which will reflect the political processes in the post-Soviet countries.
RANCANG BANGUN MODEL PERSEDIAAN DAN PEMILIHAN PEMASOK BAHAN BAKU KELAPA PARUT KERING DI PT. X
Authors: Wardah Siti , Amran Tiena Gustina , Moengin Parwadi
Published: April 2019

PT. X is an integrated agro-industry coconut company. The main products produced aredesicated coconut from coconut grain with a shelf life of 7 days as raw materials. The companycurrently constrained in 30-40% expired stock at the warehouse and insufficient capacity of thewarehouse. Another problem is the supply of raw materials (desicated coconut) from suppliersaround 20-35% did not comply with the specifications of diameter ≥ 10 cm. Seeing these conditionsrequired the model design of inventory and supplier selection with a balance of shelf life andstorage capacity as well as alternative suppliers that capable to provide raw materials (desicatedcoconut) with a diameter ≥ 10 cm continually. The method used in the design of this model is thedevelopment of methods Economic Order Quantity (EOQP) and lagrange multiplier used for theeconomic lot size ordering and fulfillment warehouse storage capacity as well as methodsAnalitycal Hierarchy Process (AHP) for the selection of alternative suppliers that meet thespecifications of raw materials and economical ordering quantity lots. The results of the design ofthis model produced economically ordering lot size 300.000 coconuts with total inventory cost Rp.55,5 million and is the District 1 alternative suppliers. In accordance with the results of analysisand expert opinion that the study design was verified and valid models.
STRATEGI TEKNOLOGI DAN MANAJEMEN INOVASI INDUSTRI TEPUNG KASAVA FERMENTASI
Authors: Misgiyarta Misgiyarta , Maarif M. Syamsul , Arkeman Yandra
Published: April 2019

Many technology research institutes and university research and development can not beapplied by the user community and industry. The result is less a role in improving the welfare ofsociety. Required critical reviews of technology and innovation management strategies togenerate technologies relevant to the needs of society and industry. The study was conducted bytaking the case of the cassava starch fermentation industry. The purpose of this study is (a)acquired technology strategy to improve the quality of fermented cassava flour, added value,competitiveness, and usability, as well as (b) obtained by way of management or management oftechnological innovation that fermented cassava flour can be applied to the industry, providingbenefits , benefits and further foster innovation. Technology strategy fermented cassava flourindustry in the form of: (1) the development of cassava starch fermentation, (2) development ofproduct diversity flour as necessary to market the composite flour technology, (3) flour over thedough expands when made, (4) development of a product of high nutritional value withfortification technology, (5) the development of a more rapid fermentation technology, (6) thedevelopment of fermented cassava flour production technology zero waste. Management ormanagement of innovation is done by: (a) search technology required product market (easy,cheap, fast), (b) selecting appropriate technology industry capacity, (c) development oftechnology that can be controlled, (d) select technology products required penndek term and longterm. Innovation management needs to be done properly so that the resulting innovation candiindustrikan, generate benefits, maximum and sustainable benefits. Management of innovationthat will either encourage the birth of new innovations that can applied in industry and providegreater benefits.
KAJIAN BAURAN PEMASARAN DAN MANAJEMEN PENGETAHUAN DALAM INOVASI KABUTO INSTAN
Authors: Sarinah Sarinah , Maarif M. Syamsul , Arkeman Yandra
Published: April 2019

Kabuto instant is a product innovation of cassava flour is dried after fermentation originSoutheast Sulawesi. New product Kabuto instant need to touch the marketing mix and knowledgemanagement (KM) in order to have a competitive edge. The concept of the marketing mix (4P) andKM, both focus on how attempts were made to support the business activities of Kabuto instantproducts to gain a competitive edge. Focus on managing the marketing mix marketing in 4 elements:(1) Product, (2) Promotion, (3) Place, and (4) Price. Management of the marketing mix will increasethe interest of consumers against products Kabuto instant. KM see the knowledge available to abusiness Kabuto instant as a major factor of success. Through knowledge of the company superiorresults can be achieved faster, cheaper and with higher quality than their competitors.Knowledge about customers, target market and other factors that may influence the likelihoodthat a faster utilization of opportunities and a more flexible response to the threat. From theperspective of a marketing mix and KM promising positive impact on the cost structure and revenuestream Kabuto instant. This paper demonstrates the advantages of using the marketing mix and theKM approach that can increase and reduce the risk of business failure by integrating bothapproaches. Management of the marketing mix requires knowledge to manage consumers, consumerknowledge and knowledge of the consumer. KM took on the role as a service provider for theconsumer, managing four (4P) in product innovation Kabuto instant. This study is based on literaturestudies.
PERBAIKAN PADA FISHBONE DIAGRAM SEBAGAI ROOT CAUSE ANALYSIS TOOL
Authors: Yuniarto Hari Agung , Akbari Annisa Dewi , Masruroh Nur Aini
Published: April 2019

Diagram Fishbone (tulang ikan), atau biasa pula disebut ishikawa diagram ataupun cause effectdiagram, adalah salah satu dari root cause analysis tools yang paling populer di kalangan praktisi industriuntuk melakukan quality improvement mendasarkan pada usaha mengenali akar penyebab terjadinya variasipada quality characteristics tertentu yang ingin dicapai. Meski telah banyak dipakai di dunia industri,disayangkan tool ini menderita kelemahan karena tidak memfasilitasi analisa korelasi antar potential rootcauses dari masing-masing kategori yang ada (5M1E - man machine method measurement materialenvironment), selain tentu saja penyajian datanya yang hanya kualitatif. Kelemahan ini diyakini menjadikontributor utama penyebab kegagalan fishbone diagram dalam mengenali root causes yang berupa sumbervariasi common cause dan hanya mampu mengenali yang berasal dari sumber variasi special cause. Bertolakbelakang dengan karakteristik special cause variations, common cause variations adalah variasi yang terjadipada quality characteristics tertentu yang ingin dicapai di mana kemunculannya tidak mudah teridentifikasidan jikapun berhasil dikenali akan sulit dihilangkan karena sifatnya yang seolah adalah merupakan bagiandari sistem (embedded in a system), cenderung berakar penyebab berupa soft factors serta kemunculannya yangtidak random namun tersamar dalam pola tertentu.Penelitian ini bertujuan melakukan improvement pada kelemahan yang terdapat di fishbone diagramdengan mengadopsi kelebihan yang dimiliki oleh bayesian network agar mampu mengenali root causes yangmerupakan common cause variations. Kelebihan bayesian network mengatasi kekurangan fishbone diagram,demikian pula sebaliknya. Oleh karena itu, analisa dilakukan terhadap fishbone diagram dan bayesian networkuntuk mengenali characteristics dan kelebihan/kekurangannya. Hasil dari analisa tersebut mengarahkan padasifat-sifat komplementer dari keduanya yang diyakini mampu mengisi gap pada fishbone diagram.Mendasarkan padanya, dikembangkan sebuah model untuk mengintegrasikan konsep serta sifat komplementeryang dimiliki bayesian network dan fishbone diagram. Model ini merepresentasikan metodologi baru dalamroot cause analysis, bayes-fishbone. Metodologi yang dikembangkan ini kemudian diujikan ke sebuah casestudy company untuk melihat applicability-nya.Hasil dari penelitian ini menunjukkan bahwa metodologi bayes-fishbone yang dikembangkan terbuktitelah valid mampu merepresentasikan kondisi probabilitas produk cacat sebenarnya pada case study companydengan prosentase perbedaan nilai yang ditunjukkan antara model yang dikembangkan dengan kondisi aktualyang besarnya tidak signifikan yaitu kurang dari 1 % (0,9597%). Dengan menerapkan metode contructiveresearch approach, terbukti pula bahwa metodologi bayes-fishbone berhasil lolos weak-market test yangmenunjukkan bahwa metodologi yang dikembangkan applicable pada case study company atau perusahaan lainyang sejenis characteristics dan production process-nya.
PENGEMBANGAN INOVASI PRODUK YANG BERDAYA SAING CYPHO JELLY DRINK
Authors: Casnan Casnan , Maarif M. Syamsul , Arkeman Yandra
Published: April 2019

Beverage industry is a segment of the food industry wich is quite the trend these
days. The industry is rapidly innovate, make changes, and have more buzzing dynamics than
the other segments. Beverage industry was seen easier to adopt scientific concepts regarding
food ingridien functionality when compared to other food industries. Beverage industry is no
longer producing beverages to satisfy thirst, but many offer the concepts and features diverse
functionality. Eggplant Netherlands (Cyphomandra betecea Sendt.) Rich in provitamin A
good for eye health and vitamin C to treat thrush and increase endurance. Essential
minerals, such as potassium, phosphorus and magnesium were able to keep and maintain
good health. High fiber of the dutch eggplant benefit prevent cancer and constipation. And
one of the advantages of other dutch eggplant is capable of lowering high blood pressure.
Beverage industry needs to be developed as the Indonesian original product that utilizes
local commodities dutch eggplant is Cypho Jelly Drink beverages functional raw materials
based local eggplant netherlands (Cyphomandra betecea Sendt.) lowering high blood
pressure.
PERBAIKAN KESEIMBANGAN LINTASAN PRODUKSI GASKET DI PT. JEIL FAJAR INDONESIADENGAN MENGGUNAKAN PENDEKATAN SIMULASI
Authors: Farid Setya Ery Achmad , Moengin Parwadi , Adisuwiryo Sucipto
Published: April 2019

Jeil Fajar Indonesiaadalah perusahaan nasional yang memproduksi Gasket. Permasalahan
yang muncul pada lantai produksi adalah sering terjadi kesulitan dalam pencapaian target produksi
tepat pada waktu yang ditentukan. Masalah ini diduga disebabkan oleh tidak seimbangnya lintasan
produksi. Hasil produksi rata-rata Gasket yang dihasilkan saat ini adalah 358 unit per minggu,
sedangkan target produksi yang ditetapkan adalah 375 unit per minggu.
Metodologi yang digunakan adalah dengan menggunakan simulasi. Data yang didapatkan
kemudian dihitung dan dianalisis beban kerja tiap stasiun. Utilitas stasiun kerja terbesar saat ini
adalah utilitas yang ada pada stasiun kerja packaging dengan persentase sebesar 99,57%. Untuk
stasiun kerja yang utilitas terkecil adalah stasiun kerja cleaning dengan persentase sebesar10,65%.
Hal ini dapat diatasi dengan 6 model usulan yang diberikan. Model usulan terbaik adalah
pemindahan 6 operator assembling ke stasiun kerja packaging.
INOVASI TEKNOLOGI NANO UNTUK COMPOSTING TANDAN KOSONG KELAPA SAWIT
Authors: Sari Vonny Indah , Maarif M. Syamsul , Arkeman Yandra
Published: April 2019

Pembuatan kompos dari tandan kosong kelapa sawit sudah banyak dilakukan dengan metode
sederhana. Beberapa penelitian yang dilakukan untuk mempercepat waktu pengomposan dilakukan
oleh Baharuddin et.al (2009) yang mengembangkan teknologi thermophillic in vessel dan windrows
system yang dapat meningkatkan komposting dari tandan kosong kelapa sawit dan palm oil mill
effluent (POME) pada skala industri. Pembuatan kompos dilakukan dengan open windrow
composting antara tandan kosong kelapa sawit yang sudah dipres dengan memberikan POME.
Melalui inovasi teknologi nano kompos tandan kosong kelapa sawit diharapkan dapat menjadi solusi
aternatif dari permasalahan karena tingginya harga pupuk non organik, peningkatan kualitas pupuk
organik dan dalam rangka mewujudkan industri PKS tanpa polusi limbah padat.
MODEL PERENCANAAN KARIR PRANATA KOMPUTER
Authors: Rachmalia Tiara
Published: April 2019

Functional position has disadvantage that for promotion of PNS not certainly, depands on
PNS can reach specified requirements. Pranata Komputer as one of functional positions also
has same disadvantage. Because of that, it needs career planning model for Pranta
Komputer so that time for promotion is decent. Career planning model designed based on
three stages of a career, which is entry stage, maturity stage, and passage stage. Career
planning model designed influenced by elements of activities, events, and credit numbers.
The verification results show that the model has been as expected. The validation result show
that activities designed for promotion have a high suitability, but for ease of implementation
differs in each rank.
IMPLEMENTASI MODEL CRISP-DM UNTUK MENENTUKAN SALES PIPELINE PADA PT X
Authors: Feblian Dina , Daihani Dadan Umar
Published: April 2019

PT X is a joint venture subsidiary of a bank in Indonesia, which is a company, engaged in
general insurance business. PT X is required to increase its share of the parent company by the
shareholders in developing its business potential. In developing the potential of the business, companies
need enough information to be analyzed further. Sources of information that can be used is the business
data of the company, both of PT X as a subsidiary and its holding company. By utilizing business data
in large numbers, the company will find a variety of information needed. For the business data
processing required a technique of data mining. In a study conducted, the implementation of the model
refers to the six stages of Cross-Industry Standard Process for Data Mining or commonly known by the
acronym CRISP-DM. In doing data mining, the six phases of the CRISP-DM is business understanding,
understanding the data, the data preparation, modeling, evaluation, and deployment. At this stage of
deployment, the report will be made by the customer segmentation by region. The expected result is the
information required in the form of a new customer pipeline.
PEMODELAN PENINGKATAN KEHANDALAN DENGAN METODE ROOT CAUSE ANALYSIS DAN FMEA PADA PERUSAHAAN PEMBANGKIT LISTRIK
Authors: Erdianto Ferry Anggita
Published: April 2019

XYZ is a company engaged in the generation of electrical energy. Reliability for power
generation companies is very vital for the company's performance appraisal is based on the readiness
of the unit to generate electricity load demand PLN (State Electricity Company). To support the
reliability of the company has designed a system that is based on the reliability maintenance (RCM).
RCM application at PT XYZ is dominated by mostly preventive maintenance based on time and baseline.
Meanwhile failures still occur marked by numerous corrective maintenance that still occur on critical
equipment, causing derating production unit. Besides the efficiency of preventive maintenance is also
doubtful, the amount of PM task that has become a waste of maintenance costs. The pattern of effective
maintenance necessary to minimize disturbance.
As a baseline, the analysis Root Cause Analysis is required to determine the priority
maintenance. After that increase reliability by using Failure Mode and Effect Analysis (FMEA) was
conducted with a focus on the criteria of occurence, severity, and detection to determine the Risk
Priority Number (RPN). To enhance decision-making systems have used system analysis history data
interference as well as MTBF and MTTR. Result of the merger of these methods are known to determine
the behavior maintenance task-a task that really affect the reliability of the unit.
MODEL SISTEM PEMILIHAN MATERIAL BAKER DENGAN METODE TOPSIS DI PT. MULTI KREASI MANDIRI
Authors: Usman Hafid , Moengin Parwadi
Published: April 2019

Football is the most popular sport in the world and it has been an amusent almost for all people
in the world. At the same time Adidas still leads the market share in the world of soccer reaching almost
to 38% then followed by the closest competitor “Nike” reaching 36% market share (Rohlman, 2013).
More than 18 years of PT. Panarub industri joins adidas as a production partner of soccer shoes. In
maintaining consistence of PT. Panarub industry has responsibility seriously for either quality or
shipment. In this way if there is a delay for the shipment of subcon company, PT. Panarub industri has
the charge to give penalty. The success of OSE of each subcon company can be viewed from the
performance of RFT process and press process in PT MKM is a process which become the trouble
process in the responsibility for OSE of PT. panarub approximately RFT monthly reaching 74,03% of
95%.
There are 17 kinds of defections causing the RFT process press doesn’t reach to 95%. Based
on the method of pareto we can get the biggest defect reaching defect open bonding approximately
defecting monthly 9.911,8 prs (RFT80,06%). That’s way we make a systematically choice model of
TOPSIS and the result of data process we get the best material baker for the best solution in Canvas
Coklat having preference value up to 0,7783.
PENGUKURAN PRODUKTIVITAS LINI PRODUKSI PRODUK PANEL BOX PT. DWIMUKTI GRAHA ELEKTRINDO DENGAN MENGGUNAKAN METODE OBJECTIVE MATRIX (OMAX) DAN PERBAIKAN PRODUKTIVITAS
Authors: Suhardini Didien , Pakpahan Arnolt Kristian , Astuti Arum Tri
Published: April 2019

PT. Dwimukti Graha Elektrindo menghasilkan produk utama Panel Box. Masalah yang
dihadapi adalah target produksi tidak tercapai. Pengukuran produktivitas dilakukan dengan metode
objective matriks (OMAX) dengan indeks produktivitas bulan Januari 0, sebagai periode dasar, bulan
Februari naik 0,56%, empat bulan berikutnya terjadi penurunan 1-1,97% secara berturut-turut.
Penyebab penurunan produktivitas adalah faktor manusia karena seringnya pergantian karyawan,
kurang training, banyak operator yang absen, mudah lelah, dan kurang teliti. Faktor metode yaitu tidak
membaca SOP, setup mesin terlalu lama, salah setup mesin. Faktor mesin karena mudah rusak,
perawatan mesin terbatas. Faktor lingkungan banyak hari libur, dan terdapat kegiatan non produktif
secara berlebihan menyebabkan kurangnya produk yang dihasilkan setiap harinya. Metode Work
Sampling digunakan untuk mengidentifikasi kegiatan produktif dan non produktif pada lantai produksi.
Hasil work sampling total kegiatan ada 24 jenis, 12 jenis kegiatan produktif, dan 12 jenis kegiatan non
produktif. Hasil pengamatan menunjukan kegiatan produktif yaitu sebesar 63,56%, dan non produktif
36,44%. Untuk mengurangi kegiatan nonproduktif dilakukan perawatan mesin secara berkala,
penggunaan Conveyor untuk pemindahan bahan, membuat toilet, menyediakan pantry dekat dengan
lantai 1 dan membuat aturan disiplin kerja. Usulan perbaikan yang bisa diterapkan adalah penetapan
aturan disiplin dengan cara kepala produksi mengawasi jalannya proses produksi secara ketat. Hasil
pengamatan kembali kegiatan produktif naik menjadi 77,8% dan non produktif turun menjadi 22,2%,
dan produktivitas bulan Juli naik 12,50%.
RANCANGAN PERBAIKAN TATA LETAK MESIN PADA LANTAI PRODUKSI PRODUK CABLE NFA2XSY-T MENGGUNAKAN PAIRWISE EXCHANGE UNTUK MEMINIMASI WAKTU PRODUKSI DAN BIAYA MATERIAL HANDLING DI PT. SUCACO
Authors: Yuniarti Nenis , Batubara Sumiharni , Moengin Parwadi
Published: April 2019

PT. SUCACO Tbk is one of the biggest corporations that produces various types of cable thatis one of them cable medium / the high voltage. In the floor production medium / the high voltage therethere are problem that appeared after evaluated using the layout checklist and material handlingchecklist sheet at PT. SUCACO Tbk those are the rising of material handling costs and the productiontime. These problem caused by the layout the floor production strategy and also the use of materialhandling who has not yet been optimized. To solve the issues then the layout have to repaired by usingpairwise exchange method. So that will get the best layout in other word, the stranding machines willbe changed by cabling machines. By using the best layout proposal, promoted model will be simulatedthen. The results that gained by production time are decrease to 696 hour which is equal to 29 workingdays and for material handling costs which was originally have amount Rp. 482.268,53 decrease to Rp.414.662,93 after layout had been restoration. At the end after repair proposal was used, the issues thathappens at production floor medium/high voltage PT.SUCACO.Tbk. has been resolved. Furthermorethe addition of repairs proposal are used as company’s consideration and evaluation, which are thesecond proposal by adding 1 unit forklift material handling and replacing manual operator with trolley,the third proposal adding 2 unit drawing machines, 1 unit stranding machine and 2 unit extrudermachines in accordance with routing sheet calculation. The last proposal or the fourth, is combiningall of the proposal becoming the one.
USULAN PENGURANGAN WAKTU SETUP MENGGUNAKAN METODE SMED SERTA PENGURANGAN WAKTU PROSES PRODUKSI DAN PERAKITAN MENGGUNAKAN METODE MOST DI PT. PANASONIC MANUFACTURING INDONESIA
Authors: Fathia Rizki Nurul , Batubara Sumiharni , Safitri Dian Mardi
Published: April 2019

PT Panasonic Manufacturing Indonesia adalah perusahaan yang bergerak dalam bidangmanufaktur yang melakukan proses produksi dan perakitan produk. Sebagai perusahaan yangmelakukan proses produksi dan perakitan produk Air Conditioner, Air Conditioner Business Unit PTPanasonic Manufacturing Indonesia seringkali terjadi permasalahan terkait pencapaian target harianproduksi untuk produk Air Conditioner Model CS-YN9RKJ. Identifikasi masalah dengan diagramishikawa menunjukkan bahwa waktu setup mesin yang lama dipengaruhi oleh lamanya waktu setupmesin Fin Press FIX 18. Sedangkan, lamanya waktu pembuatan evaporator dan perakitan produkdipengaruhi oleh metode gerakan operator yang tidak sesuai standar. Lamanya waktu pembuatanevaporator juga disebabkan oleh tata letak (layout) yang tidak berdekatan antar stasiun kerja. SMED(Single Minute Exchange of Dies) adalah salah satu metode improvement dari Lean Manufacturingyang digunakan untuk mempercepat waktu yang dibutuhkan untuk melakukan setup pergantian darimemproduksi satu jenis produk ke model produk lainnya. Metode ini digunakan untuk mengurangilamanya waktu setup mesin Fin Press FIX 18. Dari usulan perbaikan menggunakan metode SMEDdiperoleh waktu setup mesin Fin Press Fix 18 selama 931.15 detik yaitu adanya pengurangan waktusetup sebanyak 54.27%. Metode MOST (Maynard Operation Sequence Tecnique) adalah salah satuteknik predetermined time system untuk pengukuran waktu yang disusun berdasarkan urutan sub-subaktivitas atau gerakan. Metode ini digunakan untuk mengurangi waktu proses pembuatan evaporatordan perakitan Air Conditioner Model CS-YN9RKJ. Usulan perbaikan yaitu melakukan perubahangerakan dan postur tubuh operator. Selain itu, usulan perbaikan untuk proses pembuatan evaporatoradalah melakukan perubahan tata letak (layout) stasiun kerja, sedangkan untuk proses perakitanadalah mengurangi elemen kerja operator. Dari usulan perbaikan menggunakan metode MOSTdiperoleh waktu proses pembuatan evaporator selama 1082.42 detik yaitu adanya pengurangan waktusebanyak 19.47%. Sedangkan, waktu proses perakitan selama 393.27 detik yaitu adanya penguranganwaktu sebanyak 29%. Hasil usulan perbaikan menggunakan metode SMED dan MOST adalahpengurangan Manufacturing Lead Time selama 423415 detik atau adanya penguranganManufacturing Lead Time sebanyak 23 %.
UPAYA PERBAIKAN KEANDALAN ROLLER MILL DENGAN ROOT CAUSE ANALYSIS DAN PENDEKATAN PREVENTIVE MAINTENANCE
Authors: Budiharjo Budiharjo , Ikatrinasari Zulfa Fitri
Published: April 2019

In order to meet the increasing needs of electricity and steam, PT. DSS seeks to maintain thecontinuity of the process of generation and distribution, by maintaining reliability of Roller Millsystem. Based on the historical damage of the Roller Mill, then conducted this study with the aim ofimproving reliability of the Roller Mill system. Search root cause of the problem using the method ofCause and Effect Diagram and Root Cause Analysis, and corrective actions with preventivemaintenance approach. Secondary data such as operation time, down time and frequency of damageof the Roller Mill system, sourced from maintenance data of Mechanical Section. From the analysisfound that the root cause of problems-Tip Plow damage is the cause of damage spare part RollerMill. Maintenance activities with the implementation of preventive maintenance has improved thereliability of the Roller Mill system with an increase of 6.90% Operational Availability.
Anggota Dewan Redaksi
Authors: Moengin Parwadi
Published: April 2019

Ini adalah daftar Anggota Dewan Redaksi
PENINGKATAN PRODUKTIVITAS MENGGUNAKAN OBJECTIVE MATRIX DAN FAULT TREE ANALYSIS DI DIVISI ASSEMBLY MASTER CYLINDER
Authors: Gustina Amran Tiena , Yasin Marsya
Published: April 2019

Perusahaan manufaktur komponen otomotif yang menghasilkan sistem rem mobil dan motor, salah satu produk unggulan adalah Master Silinder memiliki kapasitas produksi sebanyak 116.000 pcs/bulan. Data historis selama 9 bulan menunjukkan bahwa hasil produksi masih di bawah kapasitas produksi. Perlu dilakukan peningkatan produktivitas dengan mengukur produktivitas yang ada dan mengidentifikasi penyebab dasar penghambat peningkatan tersebut. Pengukuran produktivitas dilakukan dengan metode Objective Matrix untuk enam kriteria tenaga kerja, jam kerja, energi, cacat produk, akurasi produk dan utilitas mesin yang berpengaruh dalam proses produksi. Hasil skor performansi tiap bulan didapatkan dari total skor yang diharapkan sebesar 90, rasio tenaga kerja hanya mencapai skor sebanyak 30 yang terendah terjadi pada bulan Juni dan Juli dan tertinggi pada bulan Maret. Rasio produktivitas pada bulan Juni sebesar 420.54 pcs/orang dan Juli sebesar 419.14 pcs/orang, sementara pada bulan Maret rasio produktivitasnya sebesar 442.27 pcs/orang. Dari analisa kriteria penghambat peningkatan produktivitas tenaga kerja, di identifikasi penyebab dasar terjadinya penghambatan dengan menggunakan Fault Tree Analysis. Dilakukan analisis kualitatif dan kuantitatif berdasarkan Fault Tree untuk merinci basic events yang mungkin terjadi dengan perbaikan antara lain pada regulasi lembur, penempatan pekerjaan yang sesuai, pelatihan dan penginkatan komptensiKata kunci : Fault Tree Analysis (FTA), Produktivitas, Objective Matrix (OMAX),
PENGUKURAN KINERJA SUMBER DAYA MANUSIA DENGAN HUMAN RESOURCE SCORECARD DI PT. TRIO JAYA STEEL
Authors: Lorisa Carina , Doaly Carla Olyvia
Published: April 2019

Kecakapan dan keterampilan karyawan di perusahaan dapat diketahui dengan adanya pengukuran kinerja. PT. Trio Jaya Steel merupakan perusahaan pembuatan logam berlubang yang memiliki segmen pasar cukup luas. Dimana selama ini, perusahaan belum pernah melakukan pengukuran kinerja dan belum memiliki SOP. Selain itu, tingkat pendidikan karyawan yang belum mencapai target yang ditetapkan perusahaan. Oleh karena itu, pada penelitian ini pengukuran kinerja dilakukan dengan menggunakan metode HR Scorecard yang mengacu pada keempat perspektif. Berdasarkan hasil penyebaran kuesioner dimana penilaian menggunakan Skala Likert menghasilkan 11 tujuan strategis. Pengukuran kinerja dilakukan dengan menentukan lag indicator dan lead indicator serta dilakukan pembobotan dengan menggunakan AHP untuk mengetahui bobot prioritas pada setiap kriteria dan sub kriteria pada masing-masing perspektif. Selanjutnya, dilakukan pengukuran kinerja dengan menggunakan Traffic Light System dengan mengukur pencapaian target perusahaan. Berdasarkan 11 indikator terdapat 6 indikator kinerja belum mencapai target, 1 indikator jauh di bawah target dan 4 indikator sudah mencapai target. Oleh karena itu, dalam upaya peningkatan kinerja diperlukan analisis SWOT untuk menentukan startegi atau usulan perbaikan berupa SOP dan rancangan HR Scorecard.
The Shackles of Colonialism in South African Universities: An Afrocentric Analysis
Authors: Shai Kgothatso Brucely , Legodi Lebogang T.
Published: April 2019

The transition from colonialism and apartheid to democratic South Africa in the 1990s has not been a smooth one. In this context, the argument in this article is that the remnants of colonialism and apartheid continue to be evident in South African society and in universities in particular. In this country, the chains of colonialism (and later apartheid) facilitated an educational system that benefitted the coloniser (Britain) and the white minority. With the promotion of Bantu self-government came the building of historically black universities and their separation from former historically white universities. The former have inherited a Eurocentric educational system that does not meaningfully serve the needs of the majority of Africans. This alienated educational system served as a time bomb for the 2015 Fallist movements such as #RhodesMustFall at the University of Cape Town. This later led to the #TransformNWU and #FeesMustFall campaigns among students and lecturers in the country. Based on Afrocentricity as a theoretical framework, this article seeks to analyse the transformation of South African universities from being the product of colonialism to being an envisaged barometer of African scholarship. In analysing such a transformation, the authors address the following two key questions: (1) Are South African universities meeting the needs of the society they are meant to serve? (2) How far has the transformation of universities in South Africa progressed? In answering these questions, this article relies heavily on the methods of document review and discourse analysis in the broadest sense.
Kaldıraç ve Yabancı Sahipliğin Ar-Ge Yatırımları İle Firma Performansı Üzerindeki Moderatör Etkisinin İncelenmesi: Türkiye Örneği
Authors: AYAYDIN Hasan , PALA Fahrettin , BARUT Abdulkadir
Published: April 2019

Bu çalışmanın
amacı; Borsa İstanbul’da (BİST) faaliyet gösteren imalat işletmelerinin kaldıraç
ve sahiplik yapısının AR&GE harcamaları ile firma performansı üzerine
moderatör etkisini incelemektir. Çalışmada, BİST’te 2008-2016 yılları arasında
imalat sektöründe faaliyet gösteren 125 firma incelenmiştir.  Yabancı sahiplik ve kaldıracın firma
performansı üzerindeki etkisi dinamik panel modellerinden Arellana ve Bover
(1995) tarafından geliştirilen sistem GMM kullanılarak test edilmiştir.
Çalışmanın sonucuna göre, yabancı sahipliğin AR&GE yoğunluğu ile firma
performansı arasındaki ilişkiyi olumlu yönde etkilemesine rağmen, kaldıracın
AR&GE yoğunluğu ile firma performansı arasındaki ilişkiyi olumsuz yönde
etkilediği tespit edilmiştir.
Otantik Liderlik Davranışının Örgütsel Etik İklim Üzerine Yansımaları: Kantitatif Bir Çalışma
Authors: YILMAZ Hüseyin
Published: April 2019

Bu araştırmanın temel amacı, son dönemde daha fazla ilgiyi üzerinde toplayan liderlik davranışlarından otantik liderliğin örgütsel etik iklim üzerine etkilerinin çimento sektörü kapsamında incelenmesidir. Araştırma değişkenleri olarak belirlenmiş olan otantik liderlik ve örgütsel etik iklim kavramları, örgütsel uygulamalarda sonuç verici etkili bileşenler olarak karşımıza çıkmaktadır. Çalışmada kantitatif araştırma yöntemi kullanılarak ilişkisel tarama modeline başvurulmuştur. Çalışmada Walumbwa ve arkadaşlarının (2008) geliştirdiği otantik liderlik ölçeği ile Victor ve Cullen (1998) tarafından tasarlanmış olan etik iklim ölçeğinden faydalanılmıştır. Araştırmanın evrenini, Türkiye’de çimento sektöründe faaliyet gösteren işletmeler oluşturmaktadır. Bu kapsamda İzmir ilinde faaliyetlerini devam ettiren, sektöründe üretimden satışlara önde gelen büyük firmalar arasında yer alan ve bünyesinde 912 kişiyi istihdam eden bir çimento fabrikası çalışanlarına anket uygulaması gerçekleştirilmiştir. Araştırma verilerinin analizi sürecinde toplam 131 anket değerlendirmeye alınmıştır. Araştırmanın amacı doğrultusunda yapılan analizler sonucunda; Otantik liderliğin etik iklimi %1 anlamlılık düzeyinde olumlu yönde ve güçlü bir şekilde etkilediği (β=0,396, p<.001) tespit edilmiştir. Ayrıca, araştırma değişkenlerinin alt boyutları arasındaki ilişkiler de analiz edilmiş ve otantik liderliğin alt boyutları ile etik iklimin alt boyutları arasında anlamlı ilişkiler olduğu tespit edilmiştir. Araştırma otantik liderliğin inançlı davranışları ve etik konusundaki politikaları ile örgütlerin başta etik konularda yüksek performans sergilemeleri olmak üzere, toplumun sosyal sorumlulukla ilgili beklentilerini karşılamalarında etkili olabileceğini de ortaya koymaktadır.
A New Technology Breaks Through: 1000-Litre Microbial Fuel Cell Generates Pure Water and Electricity
Authors: Heinzelmann Elsbeth
Published: April 2019

For years, researchers led by Professor Fabian Fischer at HES-SO Valais have been concentrating on microbial fuel cells, also called bio-electrochemical systems, which use electrogenic bacteria to generate electricity. Their latest innovation is a device consisting of about 14 metres
of joined-up microbial fuel cells, housed in the 'catacombs' – a series of underground tunnels – beneath the wastewater treatment plant in Sion. It uses bio-electrogenic rather than aerobic microbes for the primary purpose of producing energy and purified water, but also to save
electricity.
Rationalizing the pH-Activity Response for Escherichia Coli’s Glycinamide Ribonucleotidetransformylase Through Computational Methods
Authors: Roitberg Adrian , Lal Gupta Pancham
Published: April 2019

Human Glycinamide ribonucleotide transformylase (GAR Tfase), a regulatory enzyme in the de novo purine biosynthesis pathway, has been established as an anti-cancer target. GAR Tfase catalyzes the formyl transfer reaction from the folate cofactor to the GAR ligand. In the present work, we study E. coli GAR Tfase, which has high sequence similarity with the human GAR Tfase with most functional residues conserved. E. coli GAR Tfase exhibits structural changes and the binding of ligands that varies with pH which leads to change the rate of the formyl transfer reaction in a pH-dependent manner. Thus, the inclusion of pH becomes essential for the study of its catalytic mechanism. Experimentally, the pH-dependence of the kinetic parameter kcat is measured to evaluate the pH-range of enzymatic activity. However, insufficient information about residues governing the pH-effects on the catalytic activity leads to ambiguous assignments of the general acid and base catalysts and consequently its catalytic mechanism. In the present work, we use pH-replica exchange molecular dynamics (pH-REMD) simulations to study the effects of pH on E. coli GAR Tfase enzyme. We identify the titratable residues governing the pH-dependent conformational changes in the system. Furthermore, we filter out the protonation states which are essential in maintaining the structural integrity, keeping the ligands bound and assisting the catalysis. We reproduce the experimental pH-activity curve by computing the population of key protonation states. Moreover, we provide a detailed description of residues governing the acidic and basic limbs of the pH-activity curve.
Revealing the Elemental Distribution Within Latent Fingermarks Using Synchrotron Sourced X-Ray Fluorescence Microscopy
Authors: Boseley Rhiannon , Dorakumbura Buddhika , Howard Daryl L. , de jonge martin , Tobin Mark J. , Vongsvivut Jitraporn , ho tracey , van Bronswijk Wilhelm , Hackett Mark J. , Lewis Simon W.
Published: April 2019

Fingermarks are an important form of crime-scene trace evidence; however, their usefulness may be hampered by a variation in response or a lack of robustness in detection methods. Understanding the chemical composition and distribution within fingermarks may help explain variation in latent fingermark detection with existing methods and identify new strategies to increase detection capabilities. The majority of research in the literature describes investigation of organic components of fingermark residue, leaving the elemental distribution less well understood. The relative scarcity of information regarding the elemental distribution within fingermarks is in part due to previous unavailability of direct, micron resolution elemental mapping techniques. This capability is now provided at third generation synchrotron light sources, where X-ray Fluorescence Microscopy (XFM) provides micron or sub-micron spatial resolution and direct detection with sub-μM detection limits. XFM has been applied in this study to reveal the distribution of inorganic components within fingermark residue, including endogenous trace metals (Fe, Cu, Zn), diffusible ions (Cl-, K+, Ca2+), and exogeneous metals (Ni, Ti, Bi). This study incorporated a multi-modal approach using XFM and Infrared Microspectroscopy (IRM) analyses to demonstrate co-localisation of endogenous metals within the hydrophilic organic components of fingermark residue. Additional experiments were then undertaken to investigate how sources of exogenous metals (e.g. coins and cosmetics) may be transferred to, and distributed within latent fingermarks. Lastly, this study reports a preliminary assessment of how environmental factors such as exposure to aqueous environments may effect elemental distribution within fingermarks. Taken together, the results of this study advance our current understanding of fingermark composition and its spatial distribution of chemical components, and may help explain detection variation observed during detection of fingermarks using standard forensic protocols.
ره‌نگڤه‌دانا مۆركێ شاهدبازى و له‌شكه‌ریێ سه‌بكێ خوراسانى د شعرا كلاسیكیا كوردیدا
Authors: Mohammed Abdi H. , Khalil Kamaran I. , Adak Abd Al Rahman
Published: April 2019

هه‌رچه‌نده‌ كو سه‌رهلدانا شعرا كلاسیكیا فارسى په‌یوه‌سته‌ ب لاسایكرنا قه‌سیده‌یا عه‌ره‌بیا كه‌ڤن و گه‌له‌ك ژ ئه‌دگارێن قه‌سیده‌یا فارسیا ده‌ستپێكێ نێزیكن ژ قه‌سیده‌یا عه‌ره‌بى، لێ دگه‌ل ڤێ چه‌ندێ ژى ئه‌ڤ شعره‌ خودان كۆمه‌كا تایبه‌تمه‌ندیانه‌ كو پتر به‌رهه‌مێ ژینگه‌ها ئیرانیا قه‌سیدێنه‌، ئێك ژ ڤان تایبه‌تمه‌ندیان زالبوونا مۆركێ هه‌ڤره‌گه‌زخوازى(شاهدبازى) و له‌شكه‌ریێ كۆچكا ده‌ستهه‌لاتدارێن سامانى و غه‌زنه‌وى و سه‌لجوقى لسه‌ر ته‌غه‌زولێن ده‌ستپێكا قه‌سیده‌یانه‌. ل سه‌رده‌مێ ده‌ستهه‌لاتداریا ڤان ده‌وله‌تان و ژبه‌ر وان هێرش و داگیركاریێن كو ئه‌و پێ رادبوون؛ هه‌بوونا غولامان و بكارئینان وان بۆ مه‌به‌ستێن جۆراو جۆر و شانازیكرن ب سوپا و جه‌نگاوه‌ران ب تایبه‌تى غولامێن توركنه‌ژاد به‌شه‌كێ به‌رچاڤ ژ وێنه‌ و ده‌ربرینێن غه‌زه‌لیێن شاعرێن فارس ب خۆڤه‌ گرتیه‌. دیاره‌ دگه‌ل ده‌ربازبوونا ده‌مى و ته‌نانه‌ت پشتى لاوازبوونا قالبێ قه‌سیده‌یێ ژى شاعرێن فارس به‌رده‌وام بووینه‌ لسه‌ر لاسایكرنا ڤان تایبه‌تمه‌ندیان، هه‌تا وى راده‌ى كو پرانیا جاران ئه‌و خۆشتڤیا كو شاعرێن فارس باسدكه‌ن بریتیه‌ ژ كوڕه‌كێ جه‌نگاوه‌ر و سه‌رنجراكێش.دیاره‌ ئه‌ڤ دیارده‌یه‌ ژ ئه‌نجامێ لاسایكرنا شعرا فارسى هاتیه‌ ڤه‌گوهاستن بۆ ناڤ شعرا كوردى و گه‌له‌ك جاران شاعرێن كلاسیكیێن كورد ب ئاوا و بیانۆیێن جیاواز وه‌سفا خۆشه‌ویسته‌كێ نێر و جه‌نگاوه‌ر دكه‌ن یان ئه‌و وێنه‌یێن ئه‌و بكاردئینن به‌رهه‌مێ كاریگه‌ریا مۆركێ شاهدبازى و له‌شكه‌ریێ قه‌سیده‌یا سه‌بكێ خوراسانینه‌.
چه‌مكى ئازادى له‌ ناوه‌رۆكى پرۆگرامه‌كانى خوێندن له‌ هه‌رێمى كوردستان– بابه‌تى زمان و وێژه‌ى كوردى وه‌ك نموونه‌
Authors: Mohammed Nasraddin I.
Published: April 2019

مه‌به‌ست له‌م توێژینه‌وه‌یه‌ زانینى ئاستى بوونى چه‌مكى ئازادییه‌ له‌ ناوه‌رۆكى بابه‌تى وێژه‌ و زمانى كوردى قۆناغى ئامادییه‌ به‌ پێى پرۆگرامى خوێندنى هه‌رێمى كوردستانى عێراق، و بۆ ئه‌م مه‌به‌سته‌ توێژه‌ هه‌ڵساوه‌ به‌ دانانى لیسته‌یه‌ك له‌ جۆره‌كانى ئازادى، و دواى دابه‌شكردنى به‌سه‌ر كۆمه‌ڵێك پسپۆر و ده‌رخستنى تایبه‌تمه‌ندییه‌كانى سایكۆمێتریك، و به‌ به‌كارهێنانى شێوازى توێژینه‌وه‌ى شیكارى وه‌سفى و پێداچوون به‌ ناوه‌رۆكى سه‌مپلێك كه‌ بریتى بوو له‌ (748) لاپه‌ڕه‌ له‌ كۆى گشتى (845) لاپه‌ڕه‌ى سێ په‌رتووكى سێ قۆناغى ده‌ه و یانزده‌ه و دوانزده‌، واته‌ (89%)، ئه‌نجامه‌كان پیشانیان داوه‌ كه‌ ژماره‌ى دووباره‌بوونى ئه‌و چه‌مكه‌ (1673) جاره‌، و به‌ پێى گۆڕاوى قۆناغ و جۆره‌كانى ئازادى جیاوازییه‌كى واتادار له‌ ئاستى (0.05) له‌و چه‌مكه‌ له‌ ناوه‌رۆكى ئه‌و سێ په‌رتووكه‌ ده‌بیندرێت.
بازتاب اندیشه های فلسفی افلوطینی در مثنوی مم و زین
Authors: Rashid Jihad Sh.
Published: April 2019

احمدخانی از شاعران اندیشمندی است که از هنر شعری و داستانسرایی برای بیان اندیشه های فکری خود بهره های زیادی برده است. مثنوى مم و زین به عنوان بزرگ­ترین منظومه عاشقانه در ادبیات کردی تصویری از جهان بینی شاعر را ارائه داده است كه نسبت به ساير منظومه هاي عاشقانه دیگر در ادبیات کردی از کمال و پختگی بیشتری برخوردار است چرا که علاوه بر جنبه ی عاشقانه آن، هم بر جنبه­ی زاهدانه و معتبدانة سلوک تأکید کرده است و هم بر جنبة فلسفی که در لا به لای پنهان این منظومه کارکردی فلسفی نهفته است. بررسی اندیشه­های فلسفی و جهان بینی این شاعر که محور اصلی این پژوهش را به خود اختصاص می دهد، چهرة احمد خانی را برای ما روشن و دنیای نگرشی او را برای ما آشکارتر خواهد نمود که چه مقدار از اندیشه های فلوطینی ریشه در آثار وی داشته است. روی هم رفته اندیشیدن به اضلاع و کانونهای ارجاعی و انتزاعی منظومه مم و زین نشان می دهد که احمد خانی ماهرانه زوایای پنهان این شعر یا روایت را در منزلتی تعالی گرایانه و با رویکردی غایت شناسانه فراهم آورده است به گونه ای که مبانی بنیادین وجودشناسی را به گونه ای معرفت شناختی تبیین کرده است. این پژوهش با هدف شکافتن ریشه های اندیشه و جهان بینی خانی در اصول فکری فلوطین نگاشته شده است از این رو نگارنده در این مقاله می کوشد تا با توجه به روش استنادی، به تحلیل شاخصه های جهان نگری در آثار فلوطین و خانی بپردازد تا جهان شناسی آنان را آشکار کند.
منهج ابن آدم في كتابه مصباح الخافية في شرح نظم الكافية (بحث مستل)
Authors: Rashid Rashid A. , Mostafa Mohamed S. , Hafez al-Obeidi Abdulkarim M.
Published: April 2019

اخترت مخطوطة للعالم الكوردي المعروف ابن آدم البالكي الذي ولد في مدينة راوندوز عام (1160ه) لتكون مشروع أطروحة الدكتوراه الموسومة بـ ( مصباح الخافية في شرح نظم الكافية لابن آدم البالكي المتوفى على الراجح عام 1237ه – القسم الأول – الأسماء والممنوع من الصرف/ دراسة وتحقيق). إنّ هذه المخطوطة هي في النحو العربي، شرح مفصل لمنظومة: كفاية الطالب نظم كافية ابن الحاجب لمعاصره الشيخ معروف النودهي (ت 1254ه)، ومتن النظم هو المختصر الوجيز في النحو ( كافية ذوي الأرب في معرفة كلام العرب) لابن الحاجب الكوردي (ت 646ه). تتألف المخطوطة من 423 صفحة، (212 لوحة)، حققت ودرست (99) لوحة منها، تناولتها من خلال قسمين، وانقسم بدوره على فصلين: الأول منهما سلط الضوء على حياته ومكانته العلمية وآثاره، وخصص الفصل الثاني لدراسة المخطوطة. أمّا القسم الثاني فتناول تحقيق المخطوطة، وكان عملي فيه منصبا على إعادة كتابة المخطوطة أقرب ما يكون إلى ما أراد المؤلف، غير متصرف في النص إلا في تصحيح تحريف أو خطإ إملائي، متبعا فيه الكتابة على وفق الإملاء الحديث، وقد خرجت الآيات القرآنية، والأحاديث النبوية الشريفة، وكذلك الأبيات الشعرية والأقوال والأمثال. وأكملت ما كان ذكر منه موطن الشاهد فقط. لم يكن ابن آدم نحويا فحسب بل كان علما موسوعيا كتب في علوم مختلفة من لغة وفقه ومنطق وعروض وفلك وغيرها. ولكن لم تصلنا منها سوى 69 أثرا. توفي ابن آدم في قرية ديلزة  في راوندوز عام 1237 ه بعد عمر مليء بالعطاء.
Human CST suppresses origin licensing and promotes AND-1/Ctf4 chromatin association
Authors: Wang Yilin , Brady Kathryn S , Caiello Benjamin P , Ackerson Stephanie M , Stewart Jason A
Published: April 2019

Human CTC1-STN1-TEN1 (CST) is an RPA-like single-stranded DNA-binding protein that interacts with DNA polymerase α-primase (pol α) and functions in telomere replication. Previous studies suggest that CST also promotes replication restart after fork stalling. However, the precise role of CST in genome-wide replication remains unclear. In this study, we sought to understand whether CST alters origin licensing and activation. Replication origins are licensed by loading of the minichromosome maintenance 2–7 (MCM) complex in G1 followed by replisome assembly and origin firing in S-phase. We find that CST directly interacts with the MCM complex and disrupts binding of CDT1 to MCM, leading to decreased origin licensing. We also show that CST enhances replisome assembly by promoting AND-1/pol α chromatin association. Moreover, these interactions are not dependent on exogenous replication stress, suggesting that CST acts as a specialized replication factor during normal replication. Overall, our findings implicate CST as a novel regulator of origin licensing and replisome assembly/fork progression through interactions with MCM, AND-1, and pol α.
Mitotic replisome disassembly depends on TRAIP ubiquitin ligase activity
Authors: Priego Moreno Sara , Jones Rebecca M , Poovathumkadavil Divyasree , Scaramuzza Shaun , Gambus Agnieszka
Published: April 2019

We have shown previously that the process of replication machinery (replisome) disassembly at the termination of DNA replication forks in the S-phase is driven through polyubiquitylation of one of the replicative helicase subunits (Mcm7) by Cul2LRR1ubiquitin ligase. Interestingly, upon inhibition of this pathway inCaenorhabditis elegansembryos, the replisomes retained on chromatin were unloaded in the subsequent mitosis. Here, we show that this mitotic replisome disassembly pathway exists inXenopus laevisegg extract and we determine the first elements of its regulation. The mitotic disassembly pathway depends on the formation of K6- and K63-linked ubiquitin chains on Mcm7 by TRAIP ubiquitin ligase and the activity of p97/VCP protein segregase. Unlike in lower eukaryotes, however, it does not require SUMO modifications. Importantly, we also show that this process can remove all replisomes from mitotic chromatin, including stalled ones, which indicates a wide application for this pathway over being just a “backup” for terminated replisomes. Finally, we characterise the composition of the replisome retained on chromatin until mitosis.