bluuebunny/crossref_metadata_2025 huggingface dataset:
Pengaruh Pendidikan Kesehatan Melalui Media Audiovisual Terhadap Pengetahuan Ibu Nifas Tentang Perawatan Luka Perineum di Rumah Sakit Nurul Hasanah Aceh Tenggara 2023
Authors: Lestari Uci , Nurseptiana Eva
Published: August 2023

Masa nifas juga dapat mengalami infeksi, masa nifas masih berperan sebagai penyebab utama kematian ibu terutama di Negara berkembang seperti Indonesia ini, masalah ini terjadi akibat dari pelayanan kebidanan yang masih jauh dari sempurna. Faktor penyebab lain terjadinya infeksi nifas diantaranya, daya tahan tubuh yang kurang, kurang gizi/mal nutrisi, anemia, hygiene yang kurang baik, kelelahan serta perawatan nifas yang kurang baik seperti adanya robekan pada perineum (Kemenkes RI, 2016). Sampel adalah bagian dari populasi yang dianggap mewakili populasinya. Sampel dalam penelitian ini menggunakan total sampling yaitu sebanyak 62 responden yang ada di Rumah Sakit Nurul Hasanah 2023. pengambilan sampel dengan total sampling pelaksanaan dibulan Desember sampai dengan April 2023. Hasil Pre Test Pengetahuan Mayoritas responden memiliki Pengetahuan mayoritas pengetahuan kurang baik sebanyak 34 responden (54,8%). Ada pengaruh Pendidikan Kesehatan Melalui Media Audiovisual Terhadap Pengetahuan Ibu Nifas Tentang Perawatan Luka Perineum di Rumah Sakit Nurul Hasanah 2023. dengan nilai t = 6,681 artinya 6,681 kali Pengaruh Pendidikan Kesehatan Melalui Media Audiovisual terhadap pengetahuan ibu. Disarankan kepada rumah bersalin agar dapat dijadikan sebagai bahan masukan untuk menambah pengetahuan dalam menerapkan pengetahuan tentang kesehatan perawatan luka perineum.Kata Kunci : Media Audiovisual, Pengetahuan, Ibu NifasThe postpartum period can also experience infection, the postpartum period still plays a role as the main cause of maternal death, especially in developing countries like Indonesia, this problem occurs as a result of midwifery services which are still far from perfect. Other contributing factors to puerperal infection include poor immune system, malnutrition/malnutrition, anemia, poor hygiene, fatigue and poor postpartum care such as tears in the perineum (Ministry of Health RI, 2016). The sample is part of the population that is considered representative of the population. The sample in this study used total sampling, namely 62 respondents at Nurul Hasanah Hospital 2023. Sampling was taken with total sampling from December to April 2023. Knowledge Pre Test Results The majority of respondents had poor knowledge of the majority of knowledge as many as 34 respondents (54.8%). There is an effect of Health Education Through Audiovisual Media on Postpartum Mother's Knowledge About Perineal Wound Care at Nurul Hasanah Hospital 2023. with t = 6.681, it means 6.681 times. Effect of Health Education Through Audiovisual Media on mother's knowledge. It is suggested to the maternity hospital that it can be used as input material to increase knowledge in applying knowledge about health care for perineal wound.Keywords: Audiovisual Media, Knowledge, Postpartum Mother
Hubungan Kepatuhan Ibu Hamil Mengkonsumsi Tablet Fe dengan Anemia di Puskesmas Peusangan Kabupaten Bireuen
Authors: Nadiya Sarah , Gani Abdul , Fitria Nanda , Rizana Novia
Published: August 2023

Latar Belakang : Anemia pada ibu hamil merupakan masalah kesehatan yang serius di Indonesia. Menurut data Kementrian Kesehatan Indonesia, Prevalensi anemia pada ibu hamil di Indonesia masih tinggi, yaitu sekitar 38,3%. Anemia pada ibu hamil dapat menyebabkan berbagai komplikasi pada kehamilan seperti bayi lajir dengan berat badan rendah, kelahiran prematur, dan bahkan kematian maternal dan neonatal. Tujuan : Untuk mengetahui hubungan kepatuhan ibu hamil mengkonsumsi tablet Fe dengan kejadian anemia. Metode : Penelitian ini bersifat Analitik dengan menggunakan pendekatan Cross Sectional. Populasi dalam penelitian ini adalah seluruh ibu hamil pada bulan Desember sampai dengan Januari 2022 di Puskesmas Peusangan Kabupaten Bireuen sebanyak  43 ibu hamil. Teknik pengambilan sampel dengan cara Total Sampling sebanyak 43 jiwa. Hasil : Hasil penelitian menunjukkan adanya hubungan kepatuhan ibu hamil yang mengkonsumsi tablet Fe dengan Kejadian anemia dengan nilai p-value = 0,010< α (0,05). Kesimpulan : Dari Hasil penelitian dapat disimpulkan bahwa semakin patuh ibu hamil dalam mengkonsumsi tablet Fe selama kehamilan, maka semakin kecil ibu hamil tersebut mengalami anemia dalam kehamilan.Kata kunci : Kepatuhan Konsumsi Fe, Anemia, Ibu HamilBackground : Anemia in pregnant women is a serious health problem in Indonesia. According to data from the Indonesian Ministry of Health, the prevalence of anemia in pregnant women in Indonesia is still high, which is around 38.3%. Anemia in pregnant women can cause various complications in pregnancy such as low birth weight babies, premature births, and even maternal and neonatal deaths. Purpose : To determine the relationship between adherence of pregnant women consuming Fe tablets with the incidence of anemia. Method : This research is analytic using Cross Sectional approach. The population in this study were all pregnant women from December to January 2022 at the Peusangan Health Center in Bireuen Regency, with as many as 43 pregnant women. Sampling technique by means of a Total Sampling of as many as 43 people. Results: The results showed that there was a relationship between the compliance of pregnant women who consumed Fe tablets with the incidence of anemia with a p-value = 0.010 < α (0.05). Conclusion: From the results of the study it can be concluded that the more obedient pregnant women are in consuming Fe tablets during pregnancy,  the less pregnant women experience anemia during pregnancy.Key words : Fe Consumption Compliance, Anemia, Pregnancy
Efecto de la adición de taninos en la dieta pastoril sobre la calidad de la carne de vaca madurada
Authors: Jenko Carolina , Fabre Romina , Perlo Flavia , Biolatto Andrea , Vittone Sebastián , Tisocco Osvaldo , Bonato Patricia , Teira Gustavo
Published: August 2023

Las vacas de refugo son un subproducto de la actividad de cría. Debido a su baja condición corporal y palatabilidad de la carne, poseen un menor precio y frecuentemente son destinadas a elaboración de conservas. Se ha visto que los taninos podrían ser una estrategia nutricional alternativa a algunos moduladores ruminales, que mejorarían el perfil de aminoácidos de la carne. Además, la maduración de esta carne resaltaría sus características organolépticas, aportándole un mayor valor de mercado. El objetivo de este trabajo fue evaluar el efecto del agregado de taninos condensados en la suplementación de una dieta de terminación de vacas de refugo basada en pasturas naturales, sobre el perfil de aminoácidos, calidad sensorial  y estabilidad oxidativa de la carne madurada. Los resultados obtenidos muestran que la adición de taninos podría utilizarse en esta categoría, mejorando la terneza, sin comprometer otras características de calidad ni el contenido de aminoácidos esenciales.
Muuseumikogude potentsiaali kaardistamine päritolu-uuringute kaudu
Authors: Vider Jaanika
Published: August 2023

This article focuses on the possibilities created through provenance research in the museum. Setting out current interest in provenance research against the backdrop of antiracist and decolonial movements such as Black Lives Matter and Rhodes Must Fall, the article focuses specifically on the history of research in ethnographic museums shaped by legislative acts such as NAGPRA in the USA, critical thinking about representational practices in anthropology, and collaborative work with originating communities. Using the Siberian collection acquired by Maria Czaplicka in 1914 and held at the Pitt Rivers Museum in Oxford as a case study, I argue that broad-ranging and open-ended provenance research akin to anthropological fieldwork allows to uncover different narratives pertaining to museum objects. It enables to understand why and how the objects were brought to the museum, the kinds of epistemic realities they have helped to build but also to attend to different cultural meanings and realities embedded in these objects. A close historical study of the Czaplicka collection has added to our understanding of the nature of ethnographic research in the early 20th century, brought to attention immoral acquisition of grave goods and human remains during the Siberian expedition but also highlighted close relations between ethnographers and their Indigenous hosts. Contemporary fieldwork in Siberia and creative engagement with the collection have further broadened the understanding of the origin of these objects in relation to Evenki worldview and lived experience. Through such nuanced research, a “thick description” emerges that enables a museum interpretation that can speak to the emergence of drifts and fault lines in the society, bring different worldviews into one space, and within that space address topics of global concern.
Trend Forecasting of the Top 3 Indonesian Bank Stocks Using the ARIMA Method
Authors: Sudipa I Gede Iwan , Riana Roni , Putra I Nyoman Tri Anindia , Yanti Christina Purnama , Aristana Made Dona Wahyu
Published: August 2023

The number of investors in Indonesia increases annually. This is due to the growing popularity of investing, particularly stock investment. There are currently three largest equities in the banking industry, namely BBCA, BBRI, and BMRI. Stock prices fluctuate and form multiple patterns of price movements; therefore, investors must be able to recognize the patterns and trends of securities on the capital market in order to plan long-term investments, maximize potential profits, and reduce the risk of investment losses. In addition to knowing the trajectory of the stock market's trend, investors rely heavily on forecasting. Forecasting is necessary so that investors can anticipate future prices. The Autoregressive Integrated Moving Average (ARIMA) method is a frequently used method for forecasting time series data. In general, ARIMA is represented by the formula ARIMA (p, d, q), where p represents the Autoregressive (AR) order, d represents the difference, and q represents the Moving Average (MA) order. The trend of BBCA, BBRI, and BMRI stock data was effectively predicted using the ARIMA method. The results of this study are presented as diagrams of actual and forecasted data for the next 12 periods, as well as predictions of the optimal purchase price points for stocks. The ARIMA model of each stock also generates a low MAPE error value, with MAPE values of 4% for BBCA, 5% for BBRI, and 7% for BMRI. The MAPE value derived by each model is incorporated into the MAPE value with a high degree of precision, as it falls below 10%.
Morbilidad materna extremadamente grave.Una aproximación actual
Authors: Hernandez-Cabrera Yoan , Sosa-Osorio Alberto Antonio , Rodríguez-Duarte Luis Alberto
Published: August 2023

La morbilidad materna extremadamente grave es la condición utilizada para referirse a toda mujer que tuviera una complicación grave durante el embarazo, parto o puerperio que ponga en riesgo su vida y que requiera atención inmediata con el fin de evitar la muerte. Se realizó una búsqueda de información en las bases de datos Pubmed, Scopus, Dialnet y Lilacs. Se aplicó una estrategia de búsqueda mediante los términos: atención perinatal, hemorragia posparto, Near miss, mortalidad materna. Objetivo. Describir aspectos relacionados con la morbilidad materna extremadamente grave que permitan establecer acciones de salud encaminadas a reducir las complicaciones maternas-perinatales y consecuentemente la mortalidad materna. La hemorragia del posparto y la enfermedad hipertensiva del embarazo continúan siendo las principales causas de morbilidad materna extremadamente grave en la actualidad, lo cual varía según los criterios usados para la definición de casos y de las características de las poblaciones estudiadas en diferentes regiones del mundo. A ello se unen factores predisponentes tales como: la edad mayor de 34 años, historia previa de hemorragia posparto, embarazo múltiple, hipertensión, cesárea de emergencia, anemia, falta de control prenatal, pacientes con abortos previos y partos realizados por personas mal entrenadas. Al describir la morbilidad materna extremadamente grave como indicador que evalúa la salud materna y la calidad de su cuidado se contará con los elementos suficientes para la identificación de estos casos y la toma de decisiones referentes a la implementación de programas más efectivos que permitan la reducción de la mortalidad materna.
Relating absolute abundance of an estuarine fish to habitat area in an urbanizing environment
Authors: Rudershausen PJ , Lombardo SM , Stilson GR , O’Donnell MJ
Published: August 2023

Organisms that rely on salt marsh habitat are an important trophic link, helping to maintain estuarine ecosystem productivity. We used GIS to quantify intertidal (assumed salt marsh) area from aerial photographs taken in 1939 and from software-supplied satellite imagery taken in 2021 for tidal creeks in North Carolina (USA) that have experienced minor (<20%), moderate (20-60%), or substantial (>60%) losses of intertidal habitat over the 8 decades. The current (2022) absolute abundance of adult Fundulus heteroclitus, a trophically important resident fish in US Atlantic estuaries, was estimated over each season in each creek by fitting a Lincoln-Petersen model to tag-recapture data. Current abundances of F. heteroclitus were lowest in creeks with the lowest intertidal area. The median and 2.5/97.5 credible intervals of the posterior probability distribution for the slope of a regression model relating current fish abundance to current intertidal area were positive, demonstrating that intertidal area was a meaningful covariate of abundance. Loss of intertidal area in the creeks between 1939 and 2021 ranged from 8 to 93%. The correlation between current intertidal area and historical loss of this habitat was negative and significant (Pearson r = -0.91, p = 0.012). Parameters from the regression relating current abundance to intertidal area were used to estimate historic F. heteroclitus abundances in each creek using GIS-derived estimates of historic intertidal area. Historic abundances were predicted to have been on average (across study creeks) 7.5 times greater in 1939 than in 2022. Reduced abundances, and thus reduced trophic relay by F. heteroclitus to higher-order consumers, can be expected in estuaries that have lost salt marsh due to inter-decadal development.
Relação familiar percebida pelos irmãos de pessoas com Síndrome de Down
Authors: Palmieri Isadora Gabriella Silva , Lino Iven Giovanna Trindade , Marcon Sonia Silva
Published: August 2023

Objetivo: Conhecer a percepção da interação familiar na visão do irmão da pessoa com Síndrome de Down. Métodos: Exploratória qualitativa, realizado com irmãos de pessoas com Síndrome de Down, através de entrevistas: como é para você ter um irmão com diagnóstico de síndrome de Down? Os dados foram analisados pelo seu conteúdo. Resultados: Foram construídas as categorias temáticas: o cuidado para com o irmão com síndrome de Down, descreve como todos os irmãos desempenham algum tipo de cuidado à pessoa com SD; e descobrindo a Síndrome de Down, que conta sobre como foi o diagnóstico, as percepções dos irmãos sobre as mudanças no cenário familiar. Considerações finais: Os irmãos de desenvolvimento típico percebem a necessidade de apoio e auxílio nas demandas que uma pessoa com deficiência apresenta, assim, tendem a desenvolver maior empatia e responsabilidade, colaborando na formação e execução de cuidados com o irmão com Síndrome de Down.
Sheep welfare assessment of meat farms from Spain in different breeds and production systems
Authors: Parés Ricard , Llonch Pol , Manteca Xavier , Such Xavier
Published: August 2023

Sheep production systems are diverse in Europe, and welfare assessment should consider this diversity, including differences in breed type, climatic conditions, and time of the year. The aim of this study was to evaluate welfare in 100 commercial sheep farms in Spain, and therefore in Mediterranean conditions, using a list of 24 indicators, comprising three for good feeding, five for housing, 11 for health, and five for appropriate behavior. The list includes indicators from the AWIN protocol and other suggested indicators. Level of intensification was the main parameter that affected the welfare outcomes of feeding and health, but seasonality and flock size also affected some of the outcomes. Novel indicators included in the list (sternum evaluation, crowding behavior, subcutaneous abscesses, external parasitism, aggressive behaviors, wool pulling, ear posture, and reaction during restraint) were useful to evaluate welfare in Spanish farming conditions, but further studies should be performed to validate these indicators.
Methylation is maintained specifically at imprinting control regions but not other DMRs associated with imprinted genes in mice bearing a mutation in the Dnmt1 intrinsically disordered domain
Authors: Regmi Shaili , Giha Lana , Ali Ahado , Siebels-Lindquist Christine , Davis Tamara L.
Published: August 2023

Differential methylation of imprinting control regions in mammals is essential for distinguishing the parental alleles from each other and regulating their expression accordingly. To ensure parent of origin-specific expression of imprinted genes and thereby normal developmental progression, the differentially methylated states that are inherited at fertilization must be stably maintained by DNA methyltransferase 1 throughout subsequent somatic cell division. Further epigenetic modifications, such as the acquisition of secondary regions of differential methylation, are dependent on the methylation status of imprinting control regions and are important for achieving the monoallelic expression of imprinted genes, but little is known about how imprinting control regions direct the acquisition and maintenance of methylation at these secondary sites. Recent analysis has identified mutations that reduce DNA methyltransferase 1 fidelity at some genomic sequences but not at others, suggesting that it may function differently at different loci. We examined the impact of the mutant DNA methyltransferase 1 P allele on methylation at imprinting control regions as well as at secondary differentially methylated regions and non-imprinted sequences. We found that while the P allele results in a major reduction in DNA methylation levels across the mouse genome, methylation is specifically maintained at imprinting control regions but not at their corresponding secondary DMRs. This result suggests that DNA methyltransferase 1 may work differently at imprinting control regions or that there is an alternate mechanism for maintaining methylation at these critical regulatory regions and that maintenance of methylation at secondary DMRs is not solely dependent on the methylation status of the ICR.
Singlet-assisted diffusion-NMR (SAD-NMR): extending the scope of diffusion tensor imaging via singlet NMR
Authors: Melchiorre Giulia , Giustiniano Francesco , Rathore Sundeep , Pileio Giuseppe
Published: August 2023

In this study, long-lived nuclear singlet order methods are combined with diffusion tensor imaging with the purpose of characterizing the full diffusion tensor of molecules diffusing freely in large pores of up to a millimeter in size. Such sizes are out of reach in conventional diffusion tensor imaging because of the limitations imposed by the relaxation decay constant of the longitudinal magnetization. A singlet-assisted diffusion tensor imaging methodology able to circumvent such limitations is discussed, and the new possibilities that it offers are demonstrated through simulation and experiments on plastic phantoms containing cylindrical channels of 1 mm in diameter.
Ethnic and racial differences in Asian populations with ion channelopathies associated with sudden cardiac death
Authors: Zaveri Sahil , Qu Yongxia Sarah , Chahine Mohamed , Boutjdir Mohamed
Published: August 2023

Cardiovascular diseases are associated with several morbidities and are the most common cause of worldwide disease-related fatalities. Studies show that treatment and outcome-related differences for cardiovascular diseases disproportionately affect minorities in the United States. The emergence of ethnic and racial differences in sudden cardiac death (SCD) and related ion channelopathies complicates cardiovascular disease prevention, diagnosis, management, prognosis, and treatment objectives for patients and physicians alike. This review compiles and synthesizes current research in cardiac ion channelopathies and genetic disorders in Asian populations, an underrepresented population in cardiovascular literature. We first present a brief introduction to SCD, noting relevant observations and statistics from around the world, including Asian populations. We then examined existing differences between Asian and White populations in research, treatment, and outcomes related to cardiac ion channelopathies and SCD, showing progression in thought and research over time for each ion channelopathy. The review also identifies research that explored phenotypic abnormalities, device usage, and risk of death in Asian patients. We touch upon the unique genetic risk factors in Asian populations that lead to cardiac ion channelopathies and SCD while comparing them to White and Western populations, particularly in the United States, where Asians comprise approximately 7% of the total population. We also propose potential solutions such as improving early genetic screening, addressing barriers affecting access to medical care and device utilization, physician training, and patient education on risks.
Exploring the barriers and facilitators to implementing electronic health records in a middle-income country: a qualitative study from South Africa
Authors: Zharima Campion , Griffiths Frances , Goudge Jane
Published: August 2023

Introduction

As more countries are moving towards universal health care, middle-income countries in particular are trying to expand coverage, often using public funds. Electronic health records (EHR) are useful in monitoring patient outcomes, the performance of providers, and so the use of those public funds. With the multiple institutions or departments responsible for providing care to any individual, rather than a single record, an EHR is the interface through which to view data from a digital health information eco-system that draws on data from many different sources. South Africa plans to establish a National Health Insurance fund where EHRs will be essential for monitoring outcomes, and informing purchasing decisions. Despite various relevant policies and South Africa's relative wealth and digital capability, progress has been slow. In this paper, we explore the barriers and facilitators to implementing electronic health records in South Africa.

Methods

In this qualitative study, we conducted in-depth interviews with participants including academics, staff at parastatals, managers in the private health sector, NGO managers and government staff at various levels.

Results

The Western Cape provincial government over a 20-year period has managed to develop a digital health information ecosystem by drawing together existing data systems and building new systems. However, despite having the necessary policies in place and a number of stand-alone population level digital health information systems, several barriers still stand in the way of building national electronic health records and an efficient digital health ecosystem. These include a lack of national leadership and conflict, a failure to understand the scope of the task required to achieve scale up, insufficient numbers of technically skilled staff, failure to use the tender system to generate positive outcomes, and insufficient investment towards infrastructural needs such as hardware, software and connectivity.

Conclusion

For South Africa to have an effective electronic health record, it is important to start by overcoming the barriers to interoperability, and to develop the necessary underlying digital health ecosystem. Like the Western Cape, provincial governments need to integrate and build on existing systems as their next steps forward.
Near crushing mechanism of gangue-containing coal seams and parameter optimization for combined fully mechanized caving
Authors: Hao Xiaobo , Liu Pengcheng , Deng Yanhui , Chi Xiaolou , Li Zhihua
Published: August 2023

Fully mechanized caving is the most popular method for safe and efficient coal mining in China. In this study, based on the geological and mining conditions of Workface 822 of the Yuandian No. 1 Mine, we used theoretical analysis and numerical simulation to analyze the crushing mechanism of gangue-containing coal seam roofs, discuss the mechanical model of stability of gangue-containing coal seams to simulate different methods and caving intervals, and optimize the caving parameters of coal from gangue-containing coal seams. The results indicated that under the action of mining-induced stress, the peak pressure on the support is less than the rupture stress of the gangue, and relying on the mine support pressure alone can promote the rupture of the roof coal. However, it is difficult to rupture the gangue in the roof; owing to the gangue-containing coal seams, the area where the damage coefficient of the roof coal at the upper end of the support is lower than 1 is further expanded from the front of the support to the entire upper area of the support, and the crushing effect of roof coal is greatly weakened. After comparing the effects of five caving modes, we finally decided to use the single-cycle interval caving mode. The minimum gangue content of Workface 822 of the Yuandian No. 1 Mine is 54.35%. The results of this study can provide a theoretical basis and experience-based reference for the safe and efficient mining of gangue-containing coal seams.
Oxymatrine relieves high-fructose/fat-induced obesity via reprogramming the activity of lipid metabolism-related enhancer
Authors: Ren Luping , Liu Xuehua , Huang Xitong , Zhang He , Fei Wenjie , Yu Xian , Hu Zhijuan , Zhen Yunfeng , Chen Shuchun
Published: August 2023

Introduction

Emerging evidence demonstrates that the high-fructose and high-fat diet (HFHF) induced obesity and fatty liver disease has become one of the most common metabolic disorders worldwide. Therefore, innovative investigations on compounds targeting obesity and fatty liver diseases are urgently needed.

Methods

The high-throughput natural compounds screen was performed to screen the important compounds. A rat HFHF model was constructed, the regulatory function of Oxymatrine in HFHF-induced obesity was further explored.

Results

We identified Oxymatrine, a natural compound extracted from Sophora flavescens, showed a potential compacity in high-fat diet-induced fatty liver disease. We found that oxymatrine significantly inhibited HFHF-induced obesity using a rat HFHF model. Additionally, we found that oxymatrine altered the enhancer landscape of subcutaneous adipose tissues by ChIP-seq analysis using antibodies against the H3K27ac histone modification. Motif enrichment analysis showed the Smad motif was significantly enriched in enhancers altered post-oxymatrine treatment. Further chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR) analysis and luciferase reporter assays showed oxymatrine alters the binding of Smad3 on the enhancer regions of B-cell lymphoma 2 (Bcl2) and the enhancer activity of Bcl2.

Discussion

Together, our study highlighted oxymatrine could suppress high-fructose and high-fat diet-induced obesity by inhibiting the suppressor of mothers against decapentaplegic 3 (Smad3) binding on obesity-related enhancers.
Leveraging senescence-oxidative stress co-relation to predict prognosis and drug sensitivity in breast invasive carcinoma
Authors: Ye Yinghui , Luo Yulou , Guo Tong , Zhang Chenguang , Sun Yutian , Xu Anping , Ji Ling , Ou Jianghua , Wu Shang Ying
Published: August 2023

Introduction

Female breast cancer has risen to be the most common malignancy worldwide, causing a huge disease burden for both patients and society. Both senescence and oxidative stress attach importance to cancer development and progression. However, the prognostic roles of senescence and oxidative stress remain obscure in breast cancer. In this present study, we attempted to establish a predictive model based on senescence-oxidative stress co-relation genes (SOSCRGs) and evaluate its clinical utility in multiple dimensions.

Methods

SOSCRGs were identified via correlation analysis. Transcriptome data and clinical information of patients with breast invasive carcinoma (BRCA) were accessed from The Cancer Genome Atlas (TCGA) and GSE96058. SVM algorithm was employed to process subtype classification of patients with BRCA based on SOSCRGs. LASSO regression analysis was utilized to establish the predictive model based on SOSCRGs. Analyses of the predictive model with regards to efficacy evaluation, subgroup analysis, clinical association, immune infiltration, functional strength, mutation feature, and drug sensitivity were organized. Single-cell analysis was applied to decipher the expression pattern of key SOSCRGs in the tumor microenvironment. Additionally, qPCR was conducted to check the expression levels of key SOSCRGs in five different breast cancer cell lines.

Results

A total of 246 SOSCRGs were identified. Two breast cancer subtypes were determined based on SOSCRGs and subtype 1 showed an active immune landscape. A SOSCRGs-based predictive model was subsequently developed and the risk score was clarified as independent prognostic predictors in breast cancer. A novel nomogram was constructed and exhibited favorable predictive capability. We further ascertained that the infiltration levels of immune cells and expressions of immune checkpoints were significantly influenced by the risk score. The two risk groups were characterized by distinct functional strengths. Sugar metabolism and glycolysis were significantly upregulated in the high risk group. The low risk group was deciphered to harbor PIK3CA mutation-driven tumorigenesis, while TP53 mutation was dominant in the high risk group. The analysis further revealed a significantly positive correlation between risk score and TMB. Patients in the low risk group may also sensitively respond to several drug agents. Single-cell analysis dissected that ERRFI1, ETS1, NDRG1, and ZMAT3 were expressed in the tumor microenvironment. Moreover, the expression levels of the seven SOSCRGs in five different breast cancer cell lines were quantified and compared by qPCR respectively.

Conclusion

Multidimensional evaluations verified the clinical utility of the SOSCRGs-based predictive model to predict prognosis, aid clinical decision, and risk stratification for patients with breast cancer.
HNF4α isoforms: the fraternal twin master regulators of liver function
Authors: Radi Sarah H. , Vemuri Kiranmayi , Martinez-Lomeli Jose , Sladek Frances M.
Published: August 2023

In the more than 30 years since the purification and cloning of Hepatocyte Nuclear Factor 4 (HNF4α), considerable insight into its role in liver function has been gleaned from its target genes and mouse experiments. HNF4α plays a key role in lipid and glucose metabolism and intersects with not just diabetes and circadian rhythms but also with liver cancer, although much remains to be elucidated about those interactions. Similarly, while we are beginning to elucidate the role of the isoforms expressed from its two promoters, we know little about the alternatively spliced variants in other portions of the protein and their impact on the 1000-plus HNF4α target genes. This review will address how HNF4α came to be called the master regulator of liver-specific gene expression with a focus on its role in basic metabolism, the contributions of the various isoforms and the intriguing intersection with the circadian clock.
Wide range in estimates of hydrogen emissions from infrastructure
Authors: Esquivel-Elizondo Sofia , Hormaza Mejia Alejandra , Sun Tianyi , Shrestha Eriko , Hamburg Steven P. , Ocko Ilissa B.
Published: August 2023

Hydrogen holds tremendous potential to decarbonize many economic sectors, from chemical and material industries to energy storage and generation. However, hydrogen is a tiny, leak-prone molecule that can indirectly warm the climate. Thus, hydrogen emissions from its value chain (production, conversion, transportation/distribution, storage, and end-use) could considerably undermine the anticipated climate benefits of a hydrogen economy. Several studies have identified value chain components that may intentionally and/or unintentionally emit hydrogen. However, the amount of hydrogen emitted from infrastructure is unknown as emissions have not yet been empirically quantified. Without the capacity to make accurate direct measurements, over the past two decades, some studies have attempted to estimate total value chain and component-level hydrogen emissions using various approaches, e.g., assumptions, calculations via proxies, laboratory experiments, and theory-based models (simulations). Here, we synthesize these studies to provide an overview of the available knowledge on hydrogen emissions across value chains. Briefly, the largest ranges in estimated emissions rates are associated with liquefaction (0.15%–10%), liquid hydrogen transporting and handling (2%–20%), and liquid hydrogen refueling (2%–15%). Moreover, present and future value chain emission rate estimates vary widely (0.2%–20%). Field measurements of hydrogen emissions throughout the value chain are critically needed to sharpen our understanding of hydrogen emissions and, with them, accurately assess the climate impact of hydrogen deployment.
Load frequency control of power system based on improved AFSA-PSO event-triggering scheme
Authors: Huang Tenghao , Lv Xinxin
Published: August 2023

Aiming at the impact of redundant information transmission on network resource utilization in current power systems, an improved event-triggered scheme based on particle swarm optimization and artificial fish swarm algorithm for power system load frequency control (LFC) with renewable energy is proposed. First of all, to keep the stability and security of power systems with renewable energy, the load frequency control scheme is investigated in this paper. Then, to relieve the communication burden and increase network utilization, an improved event-triggered scheme based on the particle swarm algorithm and artificial fish swarm algorithm is explored for the power system load frequency control. Then, by utilizing improved Lyapunov functional and the linear matrix inequality method, sufficient condition for the H∞ stability of the load frequency control system is established. Finally, a two-area load frequency control system and IEEE-39 node simulation models are constructed to verify the effectiveness and applicability of the proposed method.
Immune-related gene IL17RA as a diagnostic marker in osteoporosis
Authors: Deng Ya-Jun , Li Zhi , Wang Bo , Li Jie , Ma Jun , Xue Xiong , Tian Xin , Liu Quan-Cheng , Zhang Ying , Yuan Bin
Published: August 2023

Objectives: Bone immune disorders are major contributors to osteoporosis development. This study aims to identify potential diagnostic markers and molecular targets for osteoporosis treatment from an immunological perspective.

Method: We downloaded dataset GSE56116 from the Gene Expression Omnibus database, and identified differentially expressed genes (DEGs) between normal and osteoporosis groups. Subsequently, differentially expressed immune-related genes (DEIRGs) were identified, and a functional enrichment analysis was performed. A protein-protein interaction network was also constructed based on data from STRING database to identify hub genes. Following external validation using an additional dataset (GSE35959), effective biomarkers were confirmed using RT-qPCR and immunohistochemical (IHC) staining. ROC curves were constructed to validate the diagnostic values of the identified biomarkers. Finally, a ceRNA and a transcription factor network was constructed, and a Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis was performed to explore the biological functions of these diagnostic markers.

Results: In total, 307 and 31 DEGs and DEIRGs were identified, respectively. The enrichment analysis revealed that the DEIRGs are mainly associated with Gene Ontology terms of positive regulation of MAPK cascade, granulocyte chemotaxis, and cytokine receptor. protein–protein interaction network analysis revealed 10 hub genes: FGF8, KL, CCL3, FGF4, IL9, FGF9, BMP7, IL17RA, IL12RB2, CD40LG. The expression level of IL17RA was also found to be significantly high. RT-qPCR and immunohistochemical results showed that the expression of IL17RA was significantly higher in osteoporosis patients compared to the normal group, as evidenced by the area under the curve Area Under Curve of 0.802. Then, we constructed NEAT1-hsa-miR-128-3p-IL17RA, and SNHG1-hsa-miR-128-3p-IL17RA ceRNA networks in addition to ERF-IL17RA, IRF8-IL17RA, POLR2A-IL17RA and ERG-IL17RA transcriptional networks. Finally, functional enrichment analysis revealed that IL17RA was involved in the development and progression of osteoporosis by regulating local immune and inflammatory processes in bone tissue.

Conclusion: This study identifies the immune-related gene IL17RA as a diagnostic marker of osteoporosis from an immunological perspective, and provides insight into its biological function.
Functionally distinct regions of the locus Leishmania major response 15 control IgE or IFNγ level in addition to skin lesions
Authors: Krayem Imtissal , Sohrabi Yahya , Havelková Helena , Gusareva Elena S. , Strnad Hynek , Čepičková Marie , Volkova Valeryia , Kurey Iryna , Vojtíšková Jarmila , Svobodová Milena , Demant Peter , Lipoldová Marie
Published: August 2023

Leishmaniasis, a disease caused by parasites of Leishmania spp., endangers more than 1 billion people living in endemic countries and has three clinical forms: cutaneous, mucocutaneous, and visceral. Understanding of individual differences in susceptibility to infection and heterogeneity of its pathology is largely lacking. Different mouse strains show a broad and heterogeneous range of disease manifestations such as skin lesions, splenomegaly, hepatomegaly, and increased serum levels of immunoglobulin E and several cytokines. Genome-wide mapping of these strain differences detected more than 30 quantitative trait loci (QTLs) that control the response to Leishmania major. Some control different combinations of disease manifestations, but the nature of this heterogeneity is not yet clear. In this study, we analyzed the L. major response locus Lmr15 originally mapped in the strain CcS-9 which carries 12.5% of the genome of the resistant strain STS on the genetic background of the susceptible strain BALB/c. For this analysis, we used the advanced intercross line K3FV between the strains BALB/c and STS. We confirmed the previously detected loci Lmr15, Lmr18, Lmr24, and Lmr27 and performed genetic dissection of the effects of Lmr15 on chromosome 11. We prepared the interval-specific recombinant strains 6232HS1 and 6229FUD, carrying two STS-derived segments comprising the peak linkage of Lmr15 whose lengths were 6.32 and 17.4 Mbp, respectively, and analyzed their response to L. major infection. These experiments revealed at least two linked but functionally distinct chromosomal regions controlling IFNγ response and IgE response, respectively, in addition to the control of skin lesions. Bioinformatics and expression analysis identified the potential candidate gene Top3a. This finding further clarifies the genetic organization of factors relevant to understanding the differences in the individual risk of disease.
The efficacy of botulinum toxin type A treatment and surgery for acute acquired comitant esotropia
Authors: Li Yipao , Tong Luyao , Chen Yuanyuan , Zhang BinJun , Wan Minghui , Yin Xiangping , Zhang Fang
Published: August 2023

Aim

To compare the long-term efficiency of botulinum toxin type A (BTXA) injection and surgery on acute acquired comitant esotropia (AACE).

Methods

This retrospective study enrolled patients with AACE from January 2020 to August 2022. The horizontal angle of deviation pre- and post-treatment was measured. Deviations in BTXA and surgical treatment were compared. The BTXA group was divided into adequate treatment (AT) and inadequate treatment (inAT) subgroup based on the deviation of no more than 4 prism diopters (at near and distance) or temporary exotropia at the 2 week follow-up. The two subgroups were compared to determine the long-term efficacy of BTXA treatment.

Results

Ninety-two patients with AACE were included. Follow-up was 6 months. The deviations of the surgery and BTXA group were significantly smaller at the 6 month follow-up than at pre-treatment (p < 0.001). The deviation before treatment in the surgery group was larger than in the BTXA groups (p < 0.001) but smaller at the 6 month follow-up (p < 0.001). The deviation was similar in the AT-BTXA and inAT-BTXA subgroups before treatment (p = 0.322 for distance and p = 0.051 for near) but smaller in the AT-BTXA subgroup at 6 month follow-up (p < 0.001 for near and distance).

Conclusion

Surgery and BTXA successfully treat AACE. Surgery has a more precise and lasting therapeutic effect than BTXA. AACE patients adequately treated with BTXA and with deviations of no more than 4 prism diopters at 2 weeks follow-up had better outcomes.
Lipo-chitooligosaccharide and thuricin 17 act as plant growth promoters and alleviate drought stress in Arabidopsis thaliana
Authors: Subramanian Sowmyalakshmi , Mitkus Erika , Souleimanov Alfred , Smith Donald L.
Published: August 2023

Lipo-chito-oligosaccharide (LCO–from Bradyrhizobium japonicum) and thuricin 17 (Th17–from Bacillus thuringiensis) are bacterial signal compounds from the rhizosphere of soybean that have been shown to enhance plant growth in a range of legumes and non-legumes. In this study, an attempt to quantify phytohormones involved in the initial hours after exposure of Arabidopsis thaliana to these compounds was conducted using UPLC-ESI-MS/MS. A petri-plate assay was conducted to screen for drought stress tolerance to PEG 8000 infusion and plant growth was studied 21-days post-stress. Arabidopsis thaliana plants grown in trays with drought stress imposed by water withhold were used for free proline determination, elemental analysis, and untargeted proteomics using LC-MS/MS studies. At 24 h post-exposure to the signal compounds under optimal growth conditions, Arabidopsis thaliana rosettes varied in their responses to the two signals. While LCO-treated rosettes showed a decrease in total IAA, cytokinins, gibberellins, and jasmonic acid, increases in ABA and SA was very clear. Th17-treated rosettes, on the other hand, showed an increase in IAA and SA. Both treatments resulted in decreased JA levels. Under severe drought stress imposed by PEG 8000 infusion, LCO and Th17 treatments were found to significantly increase fresh and dry weight over drought-stressed control plates, indicating that the presence of the signaling compounds decreased the negative effects experienced by the plants. Free proline content increased in LCO- and Th17-treated plants after water-withhold drought stress. Elemental analysis showed a significant increase in carbon percentage at the lower concentration of Th17. Untargeted proteomics revealed changes in the levels of drought-specific ribosomal proteins, glutathione S-transferase, late embryogenesis proteins, vegetative storage proteins 1 and 2, thaumatin-like proteins, and those related to chloroplast and carbon metabolism. The roles of some of these significantly affected proteins detected under drought stress are discussed.
Identification of disulfidptosis-related genes and subgroups in Alzheimer’s disease
Authors: Ma Shijia , Wang Dan , Xie Daojun
Published: August 2023

Background

Alzheimer’s disease (AD), a common neurological disorder, has no effective treatment due to its complex pathogenesis. Disulfidptosis, a newly discovered type of cell death, seems to be closely related to the occurrence of various diseases. In this study, through bioinformatics analysis, the expression and function of disulfidptosis-related genes (DRGs) in Alzheimer’s disease were explored.

Methods

Differential analysis was performed on the gene expression matrix of AD, and the intersection of differentially expressed genes and disulfidptosis-related genes in AD was obtained. Hub genes were further screened using multiple machine learning methods, and a predictive model was constructed. Finally, 97 AD samples were divided into two subgroups based on hub genes.

Results

In this study, a total of 22 overlapping genes were identified, and 7 hub genes were further obtained through machine learning, including MYH9, IQGAP1, ACTN4, DSTN, ACTB, MYL6, and GYS1. Furthermore, the diagnostic capability was validated using external datasets and clinical samples. Based on these genes, a predictive model was constructed, with a large area under the curve (AUC = 0.8847), and the AUCs of the two external validation datasets were also higher than 0.7, indicating the high accuracy of the predictive model. Using unsupervised clustering based on hub genes, 97 AD samples were divided into Cluster1 (n = 24) and Cluster2 (n = 73), with most hub genes expressed at higher levels in Cluster2. Immune infiltration analysis revealed that Cluster2 had a higher level of immune infiltration and immune scores.

Conclusion

A close association between disulfidptosis and Alzheimer’s disease was discovered in this study, and a predictive model was established to assess the risk of disulfidptosis subtype in AD patients. This study provides new perspectives for exploring biomarkers and potential therapeutic targets for Alzheimer’s disease.
Evaluation of computed tomography images under deep learning in the diagnosis of severe pulmonary infection
Authors: Ming Mao , Lu Na , Qian Wei
Published: August 2023

This work aimed to explore the diagnostic value of a deep convolutional neural network (CNN) combined with computed tomography (CT) images in patients with severe pneumonia complicated with pulmonary infection. A total of 120 patients with severe pneumonia complicated by pulmonary infection admitted to the hospital were selected as research subjects and underwent CT imaging scans. The empty convolution (EC) and U-net phase were combined to construct an EC-U-net, which was applied to process the CT images. The results showed that the learning rate of the EC-U-net model decreased substantially with increasing training times until it stabilized and reached zero after 40 training times. The segmentation result of the EC-U-net model for the CT image was very similar to that of the mask image, except for some deviations in edge segmentation. The EC-U-net model exhibited a significantly smaller cross-entropy loss function (CELF) and a higher Dice coefficient than the CNN algorithm. The diagnostic accuracy of CT images based on the EC-U-net model for severe pneumonia complicated with pulmonary infection was substantially higher than that of CT images alone, while the false negative rate (FNR) and false positive rate (FPR) were substantially lower (P < 0.05). Moreover, the true positive rates (TPRs) of CT images based on the EC-U-net model for patchy high-density shadows, diffuse ground glass density shadows, pleural effusion, and lung consolidation were obviously higher than those of the original CT images (P < 0.05). In short, the EC-U-net model was superior to the traditional algorithm regarding the overall performance of CT image segmentation, which can be clinically applied. CT images based on the EC-U-net model can clearly display pulmonary infection lesions, improve the clinical diagnosis of severe pneumonia complicated with pulmonary infection, and help to screen early pulmonary infection and carry out symptomatic treatment.