bluuebunny/crossref_metadata_2025 huggingface dataset:
MODERN DIDACTIC TOOLS AND THE POSSIBILITIES OF THEIR IMPLEMENTATION INTO THE EDUCATIONAL PROCESS
Authors: Klement Milan
Published: December 2021

Information and communication technologies bring a great amount of positive effects that appropriately enrich and support education. The generally declared need for the integration of new media and educational technologies in the educational process could represent an important impulse for the development of pedagogical sciences. As a result of this fact, it is necessary to promote new educational techniques and methods derived from them and thus there arises a question whether schools, teachers and teachers-to-be are ready for such a thing. Since the authors of the presented study are not aware of any available analysis dealing with this issue, we have decided, based on carrying out a research, to identify the interest and preparedness of schools or teachers, included teachers-to-be, in the implementation of modern didactic tools into the educational practice in form of interactive whiteboards. The presented study introduces the progress, process and result of this significant research.
Key words: didactic tools, digital technologies, information and communication technologies, interactive board, multimedia, multimedia presentation, research.
A VIEW ON THE LEVEL OF READING STRATEGIES OF THE SELECTED GROUP OF FIFTEEN YEARS OLD UKRAINIAN PUPILS
Authors: VICHERKOVÁ DANA
Published: December 2021

The paper presents results of the quantitative research on the level of reading strategies of fifteen years old pupils from Kyiv and Chernihiv region and their comparison with the results of Czech pupils of the same age (Vicherková, 2018). The research sample consisted of 121 subjects. The research data were obtained through the structured questionnaire. The main conclusion of the research is that Ukrainian pupils consider themselves reading literate. However, the fact contradicts the actual level of their reading strategies. The questionnaire items categorisation delimited the main factors influencing the fifteen years old Ukrainian pupils' level of reading strategies as well as the reading strategies level of the fifteen years old Czech pupils.
AULAS DE MODELAGEM MATEMÁTICA EM TEMPO DE PANDEMIA: UM RELATO DA EXPERIÊNCIA VIVIDA
Authors: Ramon Rosangela , Klüber Tiago Emanuel
Published: December 2021

Este relato busca apresentar reflexões sobre a experiência vivida ao ministrarmos a disciplina Resolução de Problemas e Modelagem Matemática alocada no terceiro ano do curso de Licenciatura em Matemática. As aulas foram realizadas no período de isolamento social devido à pandemia ocasionada pela COVID-19, de forma remota por meio da plataforma Teams. No texto, descrevemos um modo de trabalho que, partindo de tarefas de Modelagem explora aspectos teóricos da produção acadêmica da área da Modelagem, prática já realizada em aulas presenciais e que foi transposta para o ensino remoto, bem como os procedimentos de condução das aulas, as tarefas de Modelagem realizadas e algumas reflexões sobre como a prática foi realizada. Com isso, compreendemos que a abordagem adotada na disciplina se mostrou como um importante modo de proporcionar aos estudantes uma visão e uma vivência da articulação entre a teoria produzida pela comunidade acadêmica da área da Modelagem Matemática na Educação Matemática e a implementação de tarefas de Modelagem.  Por outro lado, apresentamos os anseios e as dificuldades ao ministrar uma disciplina de Modelagem em aulas remotas.
Almost 20 years of Cyclone; past, present and future
Authors: Porres Alexandre , Barber Matt , Kwan Derek
Published: December 2021

This paper discusses the history of the Cyclone library, whose purpose is to clone MAX/MSP objects into Pure Data and provide compatibility between these two environments. This paper describes the different phases and versions of Cyclone from its early days to the current state of development and our future plans.
Building in-situ instruments for the Street Sonatas #1 and #5
Authors: Roels Hans
Published: December 2021

Musical work for the Dossier “A quarter of century of Pd: past, present and future” [note by editor]
mTORC1-Mediated Angiogenesis is Required for the Development of Rosacea
Authors: Peng Qinqin , Sha Ke , Liu Yingzi , Chen Mengting , Xu San , Xie Hongfu , Deng Zhili , Li Ji
Published: December 2021

Although multiple evidences suggest that angiogenesis is associated with the pathophysiology of rosacea, its role is still in debate. Here, we showed that angiogenesis was enhanced in skin lesions of both rosacea patients and LL37-induced rosacea-like mice. Inhibition of angiogenesis alleviated LL37-induced rosacea-like features in mice. Mechanistically, we showed that mTORC1 was activated in the endothelial cells of the lesional skin from rosacea patients and LL37-induced rosacea-like mouse model. Inhibition of mTORC1 decreased angiogenesis and blocked the development of rosacea in mice. On the contrary, hyperactivation of mTORC1 increased angiogenesis and exacerbated rosacea-like phenotypes. Our in vitro results further demonstrated that inhibition of mTORC1 signaling significantly declined LL37-induced tube formation of human endothelial cells. Taken together, our findings revealed that mTORC1-mediated angiogenesis responding to LL37 might be essential for the development of rosacea and targeting angiogenesis might be a novel potential therapy.
Food-Derived High Arginine Peptides Promote Spermatogenesis Recovery in Busulfan Treated Mice
Authors: Liu Wenwen , Zhang Lingfeng , Gao Anning , Khawar Muhammad Babar , Gao Fengyi , Li Wei
Published: December 2021

Food-derived peptides with high arginine content have important applications in medicine and food industries, but their potential application in the treatment of oligoasthenospermia remains elusive. Here, we report that high-arginine peptides, such as Oyster peptides and Perilla purple peptides were able to promote spermatogenesis recovery in busulfan-treated mice. We found that both Opp and Ppp could increase sperm concentration and motility after busulfan-induced testicular damage in mice. Further research revealed that Opp and Ppp might promote spermatogonia proliferation, which improved blood-testis barrier recovery between Sertoli cells. Taken together, these high-arginine peptides might be used as a medication or therapeutic component of a diet prescription to improve the fertility of some oligoasthenospermia patients.
Removal Study of Crystal Violet and Methylene Blue From Aqueous Solution by Activated Carbon Embedded Zero-Valent Iron: Effect of Reduction Methods
Authors: Wang Yongmei , Chen Tiantian , Zhang Xiaolin , Mwamulima Teza
Published: December 2021

Zero valent iron (ZVI) particles were embedded into porous materials to avoid aggregation and separation problems in the controlled synthesis process. To investigate the adsorption mechanism of crystal violet and methylene blue, activated carbon (AC) and AC-based ZVI extraction by solid-phase and liquid-phase reduced approaches was conducted. Characterization methods of specific surface area, scanning electron microscopy (SEM), and x-ray diffractograms (XRD) were used to elucidate the structure of adsorbents, and the adsorption capacities of crystal violet and methylene blue were obtained under experimental conditions of various pH values (2.0–10.0), adsorption times (0–72 h), and temperatures (30–50°C). The adsorption of crystal violet/methylene blue was controlled by both chemisorption and reduction. The adsorption processes were fitted to a pseudo-second-order kinetic model, and that of reduction kinetics was suitable to pseudo-first-order kinetic model. The thermodynamic study revealed that the adsorption of crystal violet and methylene blue was endothermic and spontaneous, and the adsorption isotherms fitted well to the Langmuir model. Different adsorption capacities of crystal violet and methylene blue on various adsorbents were found, indicating that both the properties of adsorbents (pore size, specific surface area, and chemical functional groups) and the structures of adsorbates had significant effect on the removal of dye molecules.
RETRACTED: The Role of Janus Kinase/STAT3 Pathway in Hematologic Malignancies With an Emphasis on Epigenetics
Authors: Zeinalzadeh Elham , Valerievich Yumashev Alexey , Rahman Heshu Sulaiman , Marofi Faroogh , Shomali Navid , Kafil Hossein Samadi , Solali Saeed , Sajjadi-Dokht Mehdi , Vakili-Samiani Sajjad , Jarahian Mostafa , Hagh Majid Farshdousti
Published: December 2021

The Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signaling pathway has been known to be involved in cell growth, cellular differentiation processes development, immune cell survival, and hematopoietic system development. As an important member of the STAT family, STAT3 participates as a major regulator of cellular development and differentiation-associated genes. Prolonged and persistent STAT3 activation has been reported to be associated with tumor cell survival, proliferation, and invasion. Therefore, the JAK-STAT pathway can be a potential target for drug development to treat human cancers, e.g., hematological malignancies. Although STAT3 upregulation has been reported in hematopoietic cancers, protein-level STAT3 mutations have also been reported in invasive leukemias/lymphomas. The principal role of STAT3 in tumor cell growth clarifies the importance of approaches that downregulate this molecule. Epigenetic modifications are a major regulatory mechanism controlling the activity and function of STAT3. So far, several compounds have been developed to target epigenetic regulatory enzymes in blood malignancies. Here, we discuss the current knowledge about STAT3 abnormalities and carcinogenic functions in hematopoietic cancers, novel STAT3 inhibitors, the role of epigenetic mechanisms in STAT3 regulation, and targeted therapies, by focusing on STAT3-related epigenetic modifications.
Oil as an Enabler for Efficient Materials Removal in Three-Dimensional Scanning Probe Microscopy Applications
Authors: Noël Céline , Wouters Lennaert , Paredis Kristof , Celano Umberto , Hantschel Thomas
Published: December 2021

The ever-increasing complexity of semiconductor devices requires innovative three-dimensional materials characterization techniques for confined volumes. Multiple atomic force microscopy (AFM)-based methodologies, using a slice-and-measure approach have been proposed to meet this demand. They consist of scanning AFM probes that erode locally the sample’s material at a relatively high load while sensing with the secondary AFM channel, thus accessing in-depth information compared to the standard surface-limited analysis. Nonetheless, the rapid tip apex wear caused by the high forces involved, and the debris accumulation at the tip apex and inside/around the scan area, have been identified as major limitations to the accuracy and repeatability of the existing tomographic AFM sensing methods. Here we explore the use of oil as a suitable medium to overcome some of the issues such as the scan debris accumulation and the removal variability when working in air. We show how the use of oil preserves the tomographic operation while improving the efficiency in material removal for large depth sensing at a reduced debris accumulation. This is reported by comparing the results between air and oil environments, where the removal rate, depth accuracy, and tip-contamination are benchmarked. Finally, we provide the first demonstration of electrical AFM sensing using scanning spreading resistance microscopy (SSRM) in oil.
Lipid Alterations in Systemic Sclerosis
Authors: Gogulska Zuzanna , Smolenska Zaneta , Turyn Jacek , Mika Adriana , Zdrojewski Zbigniew
Published: December 2021

Background: Systemic sclerosis (SSc) is an autoimmune disease with an elusive etiology and poor prognosis. Due to its diverse clinical presentation, a personalized approach is obligatory and needs to be based on a comprehensive biomarker panel. Therefore, particular metabolomic studies are necessary. Lipidomics addressed these issues and found disturbances in several crucial metabolic pathways.

Aim of Review: The review aims to briefly summarize current knowledge related to lipid alterations in systemic sclerosis, highlight its importance, and encourage further research in this field.

Key Scientific Concepts of Review: In this review, we summarized the studies on the lipidomic pattern, fatty acids, lipoproteins, cholesterol, eicosanoids, prostaglandins, leukotrienes, lysophospholipids, and sphingolipids in systemic sclerosis. Researchers demonstrated several alternate aspects of lipid metabolism. As we aimed to present our findings in a comprehensive view, we decided to divide our findings into three major groups: “serum lipoproteins,” “fatty acids and derivatives,” and “cellular membrane components,” as we do believe they play a prominent role in SSc pathology.
Src Family Kinases Inhibition Ameliorates Hypoxic-Ischemic Brain Injury in Immature Rats
Authors: Qiu Han , Qian Tianyang , Wu Tong , Gao Ting , Xing Qinghe , Wang Laishuan
Published: December 2021

Hypoxic-ischemic (HI) injury is one of the initial factors contributing to neonatal brain injury. Src family kinases (SFKs) are considered to act as molecular hubs for N-methyl-d-aspartate receptor (NMDAR) regulation and participate in the HI injury process. The objectives of this study were to evaluate the levels of phospho-Src (p-Src), the relationship between NMDARs and SFKs, and the effects of SFK inhibition on an immature rat HI brain injury model. The model was induced in 3-day-old Sprague–Dawley rats using the Rice-Vannucci model operation. The level of p-Src was evaluated using Western blotting. The association of NMDARs with SFKs was detected using Western blotting and coimmunoprecipitation. After intraperitoneal injection of 4-amino-5-(4-chlorophenyl)-7-(t-butyl) pyrazolo [3,4-d] pyrimidine (PP2), an SFK-selective inhibitor, neuropathological changes were observed by performing H&E and immunofluorescence staining, and the neurological functions were assessed using the following behavioral tests: modified neurological severity score, open field test, and Morris water maze test. The levels of p-Src first decreased at 0 h after injury, increased at 2 h after injury, and continuously decreased from 6 h to 3 days. Along with the increased p-Src levels observed at 2 h after injury, the phosphorylation of NMDAR subunit NR2B at tyrosine 1472 was increased. Following the administration of PP2, the increased p-Src and NMDAR-2B levels detected at 2 h after injury were decreased, and tissue injury and myelin basic protein expression were improved at 7 days after injury. The PP2 intervention improved the performance of injured rats on behavioral tests. In conclusion, we determined the patterns of p-Src expression after HI brain injury in immature rats and showed a relationship with the activated NMDA receptor. The inhibition of p-Src ameliorates neuropathological changes and damages neurological functions induced by HI injury.
Application Value of the Motor Unit Number Index in Patients With Kennedy Disease
Authors: Zhang Shuo , Yang Xin , Xu Yingsheng , Luo Yongmei , Fan Dongsheng , Liu Xiaoxuan
Published: December 2021

The aim of this study was to evaluate the usefulness of the motor unit number index (MUNIX) technique in Kennedy disease (KD) and test the correlation between the MUNIX and other clinical parameters. The MUNIX values of the bilateral deltoid, abductor digiti minimi (ADM), quadriceps femoris (QF), and tibialis anterior (TA) were determined and compared with the course of the disease. The MUNIX sum score was calculated by adding the MUNIX values of these 8 muscles. Disability was evaluated using the spinal and bulbar muscular atrophy functional rating scale (SBMAFRS). The MUNIX scores of patients with KD were negatively correlated with the course of the disease (p < 0.05), whereas their motor unit size index (MUSIX) scores were positively correlated with the course the of disease (p < 0.05). MUNIX sum scores were correlated with SBMAFRS scores (r = 0.714, p < 0.05). MUNIX was more sensitive than compound muscle action potentials or muscle strength as an indicator of neuron loss and axonal collateral reinnervation. The MUNIX sum score is an objective and a reliable indicator of disease progression, and it is a potential choice for therapeutic clinical trials. The MUNIX can assess the functional loss of motor axons and is correlated with disability. The MUNIX sum score may be especially suitable as an objective parameter.
Neural Measures of Pitch Processing in EEG Responses to Running Speech
Authors: Bachmann Florine L. , MacDonald Ewen N. , Hjortkjær Jens
Published: December 2021

Linearized encoding models are increasingly employed to model cortical responses to running speech. Recent extensions to subcortical responses suggest clinical perspectives, potentially complementing auditory brainstem responses (ABRs) or frequency-following responses (FFRs) that are current clinical standards. However, while it is well-known that the auditory brainstem responds both to transient amplitude variations and the stimulus periodicity that gives rise to pitch, these features co-vary in running speech. Here, we discuss challenges in disentangling the features that drive the subcortical response to running speech. Cortical and subcortical electroencephalographic (EEG) responses to running speech from 19 normal-hearing listeners (12 female) were analyzed. Using forward regression models, we confirm that responses to the rectified broadband speech signal yield temporal response functions consistent with wave V of the ABR, as shown in previous work. Peak latency and amplitude of the speech-evoked brainstem response were correlated with standard click-evoked ABRs recorded at the vertex electrode (Cz). Similar responses could be obtained using the fundamental frequency (F0) of the speech signal as model predictor. However, simulations indicated that dissociating responses to temporal fine structure at the F0 from broadband amplitude variations is not possible given the high co-variance of the features and the poor signal-to-noise ratio (SNR) of subcortical EEG responses. In cortex, both simulations and data replicated previous findings indicating that envelope tracking on frontal electrodes can be dissociated from responses to slow variations in F0 (relative pitch). Yet, no association between subcortical F0-tracking and cortical responses to relative pitch could be detected. These results indicate that while subcortical speech responses are comparable to click-evoked ABRs, dissociating pitch-related processing in the auditory brainstem may be challenging with natural speech stimuli.
Application of CRISPR/Cas9 in Alzheimer’s Disease
Authors: Lu Likui , Yu Xi , Cai Yongle , Sun Miao , Yang Hao
Published: December 2021

Alzheimer’s disease (AD) is a progressive and irreversible neurodegenerative disorder clinically characterized by cognitive impairment, abnormal behavior, and social deficits, which is intimately linked with excessive β-amyloid (Aβ) protein deposition along with many other misfolded proteins, neurofibrillary tangles formed by hyperphosphorylated tau protein aggregates, and mitochondrial damage in neurons, leading to neuron loss. Currently, research on the pathological mechanism of AD has been elucidated for decades, still no effective treatment for this complex disease was developed, and the existing therapeutic strategies are extremely erratic, thereby leading to irreversible and progressive cognitive decline in AD patients. Due to gradually mental dyscapacitating of AD patients, AD not only brings serious physical and psychological suffering to patients themselves, but also imposes huge economic burdens on family and society. Accordingly, it is very imperative to recapitulate the progress of gene editing-based precision medicine in the emerging fields. In this review, we will mainly focus on the application of CRISPR/Cas9 technique in the fields of AD research and gene therapy, and summarize the application of CRISPR/Cas9 in the aspects of AD model construction, screening of pathogenic genes, and target therapy. Finally, the development of delivery systems, which is a major challenge that hinders the clinical application of CRISPR/Cas9 technology will also be discussed.
Potential Clinical Value of Pretreatment De Ritis Ratio as a Prognostic Biomarker for Renal Cell Carcinoma
Authors: Li Jinze , Cao Dehong , Peng Lei , Meng Chunyang , Xia Zhongyou , Li Yunxiang , Wei Qiang
Published: December 2021

Background

We performed this study to explore the prognostic value of the pretreatment aspartate transaminase to alanine transaminase (De Ritis) ratio in patients with renal cell carcinoma (RCC).

Methods

PubMed, EMBASE, Web of Science, and Cochrane Library were searched to identify all studies. The hazard ratio (HR) with a 95% confidence interval (CI) for overall survival (OS) and cancer-specific survival (CSS) were extracted to evaluate their correlation.

Results

A total of 6,528 patients from 11 studies were included in the pooled analysis. Patients with a higher pretreatment De Ritis ratio had worse OS (HR = 1.41, p < 0.001) and CSS (HR = 1.59, p < 0.001). Subgroup analysis according to ethnicity, disease stage, cutoff value, and sample size revealed that the De Ritis ratio had a significant prognostic value for OS and CSS in all subgroups.

Conclusions

The present study suggests that an elevated pretreatment De Ritis ratio is significantly correlated with worse survival in patients with RCC. The pretreatment De Ritis ratio may serve as a potential prognostic biomarker in patients with RCC, but further studies are warranted to support these results.
A Data Self-Calibration Method Based on High-Density Parallel Plate Diffuse Optical Tomography for Breast Cancer Imaging
Authors: Wang Xin , Hu Rui , Wang Yirong , Yan Qiang , Wang Yihan , Kang Fei , Zhu Shouping
Published: December 2021

When performing the diffuse optical tomography (DOT) of the breast, the mismatch between the forward model and the experimental conditions will significantly hinder the reconstruction accuracy. Therefore, the reference measurement is commonly used to calibrate the measured data before the reconstruction. However, it is complicated to customize corresponding reference phantoms based on the breast shape and background optical parameters of different subjects in clinical trials. Furthermore, although high-density (HD) DOT configuration has been proven to improve imaging quality, a large number of source-detector (SD) pairs also increase the difficulty of multi-channel correction. To enhance the applicability of the breast DOT, a data self-calibration method based on an HD parallel-plate DOT system is proposed in this paper to replace the conventional relative measurement on a reference phantom. The reference predicted data can be constructed directly from the measurement data with the support of the HD-DOT system, which has nearly a hundred sets of measurements at each SD distance. The proposed scheme has been validated by Monte Carlo (MC) simulation, breast-size phantom experiments, and clinical trials, exhibiting the feasibility in ensuring the quality of the DOT reconstruction while effectively reducing the complexity associated with relative measurements on reference phantoms.
Convolutional Neural Network-Based Diagnostic Model for a Solid, Indeterminate Solitary Pulmonary Nodule or Mass on Computed Tomography
Authors: Sun Ke , Chen Shouyu , Zhao Jiabi , Wang Bin , Yang Yang , Wang Yin , Wu Chunyan , Sun Xiwen
Published: December 2021

Purpose

To establish a non-invasive diagnostic model based on convolutional neural networks (CNNs) to distinguish benign from malignant lesions manifesting as a solid, indeterminate solitary pulmonary nodule (SPN) or mass (SPM) on computed tomography (CT).

Method

A total of 459 patients with solid indeterminate SPNs/SPMs on CT were ultimately included in this retrospective study and assigned to the train (n=366), validation (n=46), and test (n=47) sets. Histopathologic analysis was available for each patient. An end-to-end CNN model was proposed to predict the natural history of solid indeterminate SPN/SPMs on CT. Receiver operating characteristic curves were plotted to evaluate the predictive performance of the proposed CNN model. The accuracy, sensitivity, and specificity of diagnoses by radiologists alone were compared with those of diagnoses by radiologists by using the CNN model to assess its clinical utility.

Results

For the CNN model, the AUC was 91% (95% confidence interval [CI]: 0.83–0.99) in the test set. The diagnostic accuracy of radiologists with the CNN model was significantly higher than that without the model (89 vs. 66%, P<0.01; 87 vs. 61%, P<0.01; 85 vs. 66%, P=0.03, in the train, validation, and test sets, respectively). In addition, while there was a slight increase in sensitivity, the specificity improved significantly by an average of 42% (the corresponding improvements in the three sets ranged from 43, 33, and 42% to 82, 78, and 84%, respectively; P<0.01 for all).

Conclusion

The CNN model could be a valuable tool in non-invasively differentiating benign from malignant lesions manifesting as solid, indeterminate SPNs/SPMs on CT.
Rab11-FIP1/RCP Functions as a Major Signalling Hub in the Oncogenic Roles of Mutant p53 in Cancer
Authors: von Grabowiecki Yannick , Phatak Vinaya , Aschauer Lydia , Muller Patricia A. J.
Published: December 2021

Rab11-FIP1 is a Rab effector protein that is involved in endosomal recycling and trafficking of various molecules throughout the endocytic compartments of the cell. The consequence of this can be increased secretion or increased membrane expression of those molecules. In general, expression of Rab11-FIP1 coincides with more tumourigenic and metastatic cell behaviour. Rab11-FIP1 can work in concert with oncogenes such as mutant p53, but has also been speculated to be an oncogene in its own right. In this perspective, we will discuss and speculate upon our observations that mutant p53 promotes Rab11-FIP1 function to not only promote invasive behaviour, but also chemoresistance by regulating a multitude of different proteins.
Transcriptomic Analysis Reveals the Protective Effects of Empagliflozin on Lipid Metabolism in Nonalcoholic Fatty Liver Disease
Authors: Ma Yuting , Kan Chengxia , Qiu Hongyan , Liu Yongping , Hou Ningning , Han Fang , Shi Junfeng , Sun Xiaodong
Published: December 2021

Empagliflozin is a novel type of sodium-glucose cotransporter two inhibitor with diverse beneficial effects in the treatment of nonalcoholic fatty liver disease (NAFLD). Although empagliflozin impacts NAFLD by regulating lipid metabolism, the underlying mechanism has not been fully elucidated. In this study, we investigated transcriptional regulation pathways affected by empagliflozin in a mouse model of NAFLD. In this study, NAFLD was established in male C57BL/6J mice by administration of a high-fat diet; it was then treated with empagliflozin and whole transcriptome analysis was conducted. Gene expression levels detected by transcriptome analysis were then verified by quantitative real-time polymerase chain reaction, protein levels detected by Western Blot. Differential expression genes screened from RNA-Seq data were enriched in lipid metabolism and synthesis. The Gene Set Enrichment Analysis (GSEA) results showed decreased lipid synthesis and improved lipid metabolism. Empagliflozin improved NAFLD through enhanced triglyceride transfer, triglyceride lipolysis and microsomal mitochondrial β-oxidation. This study provides new insights concerning the mechanisms by which sodium-glucose cotransporter two inhibitors impact NAFLD, particularly in terms of liver lipid metabolism. The lipid metabolism-related genes identified in this experiment provide robust evidence for further analyses of the mechanism by which empagliflozin impacts NAFLD.
Diammonium Glycyrrhizinate Ameliorates Obesity Through Modulation of Gut Microbiota-Conjugated BAs-FXR Signaling
Authors: Li Yun , Hou Huiqin , Wang Xianglu , Dai Xin , Zhang Wanru , Tang Qiang , Dong Yue , Yan Chen , Wang Bangmao , Li Zhengxiang , Cao Hailong
Published: December 2021

Obesity is a worldwide epidemic metabolic disease. Gut microbiota dysbiosis and bile acids (BAs) metabolism disorder are closely related to obesity. Farnesoid X-activated receptor (FXR), served as a link between gut microbiota and BAs, is involved in maintaining metabolic homeostasis and regulating glucose and lipid metabolism. We previously reported that diammonium glycyrrhizinate (DG) could alter gut microbiota and prevent non-alcoholic fatty liver disease. However, it remains ambiguous how DG affects the gut microbiota to regulate host metabolism. In this present study, 16S rRNA Illumina NovaSeq and metabolomic analysis revealed that DG treatment suppressed microbes associated with bile-salt hydrolase (BSH) activity, which, in turn, increased the levels of taurine-conjugated BAs accompanied by inhibition of ileal FXR-FGF15 signaling. As a result, several obesity-related metabolism were improved, like lower serum glucose and insulin levels, increased insulin sensitivity, few hepatic steatosis and resistance to weight gain. Additionally, decreased level of serum lipopolysaccharide was observed, which contributed to a strengthened intestinal barrier. The effect of DG on weight loss was slightly enhanced in the antibiotics-treated obese mice. Collectively, the efficacy of DG in the treatment of obesity might depend on gut microbiota-conjugated BAs-FXR axis. Hence, it will provide a potential novel approach for the treatment of obesity.
Glucocorticoid-Mediated Developmental Programming of Vertebrate Stress Responsivity
Authors: Gans Ian M. , Coffman James A.
Published: December 2021

Glucocorticoids, vertebrate steroid hormones produced by cells of the adrenal cortex or interrenal tissue, function dynamically to maintain homeostasis under constantly changing and occasionally stressful environmental conditions. They do so by binding and thereby activating nuclear receptor transcription factors, the Glucocorticoid and Mineralocorticoid Receptors (MR and GR, respectively). The GR, by virtue of its lower affinity for endogenous glucocorticoids (cortisol or corticosterone), is primarily responsible for transducing the dynamic signals conveyed by circadian and ultradian glucocorticoid oscillations as well as transient pulses produced in response to acute stress. These dynamics are important determinants of stress responsivity, and at the systemic level are produced by feedforward and feedback signaling along the hypothalamus-pituitary–adrenal/interrenal axis. Within receiving cells, GR signaling dynamics are controlled by the GR target gene and negative feedback regulator fkpb5. Chronic stress can alter signaling dynamics via imperfect physiological adaptation that changes systemic and/or cellular set points, resulting in chronically elevated cortisol levels and increased allostatic load, which undermines health and promotes development of disease. When this occurs during early development it can “program” the responsivity of the stress system, with persistent effects on allostatic load and disease susceptibility. An important question concerns the glucocorticoid-responsive gene regulatory network that contributes to such programming. Recent studies show that klf9, a ubiquitously expressed GR target gene that encodes a Krüppel-like transcription factor important for metabolic plasticity and neuronal differentiation, is a feedforward regulator of GR signaling impacting cellular glucocorticoid responsivity, suggesting that it may be a critical node in that regulatory network.
The Use of Stable Zinc Isotope Soil Labeling to Assess the Contribution of Complex Organic Fertilizers to the Zinc Nutrition of Ryegrass
Authors: Yan Bo-Fang , Dürr-Auster Thilo , Frossard Emmanuel , Wiggenhauser Matthias
Published: December 2021

Manure and sewage sludge are known to add significant amounts of zinc (Zn) and other metals to soils. However, there is a paucity of information on the fate of Zn that derives from complex organic fertilizers in soil–plant systems and the contribution of these fertilizers to the Zn nutrition of crops. To answer these questions, we grew Italian ryegrass in the presence of ZnSO4, sewage sludge, and cattle and poultry manure in an acidic soil from Heitenried, Switzerland, and an alkaline soil from Strickhof, Switzerland, where the isotopically exchangeable Zn had been labeled with 67Zn. This allowed us to calculate the fraction of Zn in the shoots that was derived from fertilizer, soil, and seed over 4 successive cuts. In addition, we measured the 67Zn:66Zn isotope ratio with the diffusive gradients in thin films technique (DGT) on soils labeled with 67Zn and incubated with the same fertilizers. After 48 days of growth, the largest fraction of Zn in the ryegrass shoots was derived from the soil (79–88%), followed by the Zn-containing fertilizer (11–20%); the least (<2.3%) came from the seed. Only a minor fraction of the Zn applied with the fertilizer was transferred to the shoots (4.7–12%), which indicates that most of the freshly added Zn remained in the soil after one crop cycle and may thereby contribute to a residual Zn pool in the soil. The 67Zn:66Zn isotope ratios in the DGT extracts and the shoots measured at cut 4 were identical, suggesting that the DGT and plant took up Zn from the same pool. The proportion of Zn derived from the fertilizers in the DGT extracts was also identical to that measured in ryegrass shoots at cut 4. In conclusion, this work shows that stable Zn isotope labeling of the soil available Zn can be used to precisely quantify the impact of complex organic fertilizers on the Zn nutrition of crops. It also demonstrates that DGT extractions on labeled soils could be used to estimate the contribution of Zn fertilizers to plant nutrition.
Natural Bioactive Compounds of Sechium spp. for Therapeutic and Nutraceutical Supplements
Authors: Iñiguez-Luna María Isabel , Cadena-Iñiguez Jorge , Soto-Hernández Ramón Marcos , Morales-Flores Francisco Javier , Cortes-Cruz Moisés , Watanabe Kazuo N.
Published: December 2021

Natural products are in great demand because certain secondary metabolites (SMs) are sources of antioxidants, flavorings, active substances, or anticancer agents with less aggressiveness and selectivity, among which triterpenes and flavonoids are of importance because they inhibit carcinogenesis. For Sechium spp. P. Br. (chayotes), there is scientific evidence of antiproliferative activity that has occurred when cancer cell lines have been treated with this fruit. In order to compare future therapeutic designs and identify new and ancestral characteristics, triterpenes and flavonoids were determined in contrasting Sechium genotypes. The obtained data were analyzed via a cladistics approach, with the aim of identifying the characteristics and state of phytochemicals and genetic variables. The concentrations of flavonoids and triterpenes were determined, and a more complex composition of secondary metabolites was found in the wild types as compared to their domesticated genotypes. Bitter fruits contained a higher number of SMs, followed by those with a neutral and sweet flavor. A cladogram showed the differentiation of the three groups based on the flavor of the fruits. The diversity of SMs decreases in evolutionary terms, in response to domestication and environmental adaptation. Therefore, genotypes can be feasibly selected based on fruit flavor for gross-breeding, and cytotoxicity can be reduced without losing possible therapeutic effects.
Impact of Multimorbidity on Symptoms of Depression, Anxiety, and Stress in Older Adults: Is There a Sex Difference?
Authors: Lin Huang , Xiao Shujuan , Shi Lei , Zheng Xiao , Xue Yaqing , Yun Qilong , Ouyang Ping , Wang Dong , Zhu Hong , Zhang Chichen
Published: December 2021

Introduction: Multimorbidity has become a key issue in the health care sector globally, and it can also lead to psychological distress in older adults. This study aimed to assess the impact of multimorbidity on depression, anxiety, and stress symptoms and identify whether there is a sex difference in these associations.

Methods: A cross-sectional study using a multistage random sampling method was conducted among 3,266 older adults in China. Multiple linear regression models were used to estimate the independent associations between multimorbidity and depression, anxiety, and stress symptoms. Furthermore, interaction analysis was employed to investigate the interaction effect of multimorbidity and sex on depression, anxiety, and stress symptoms.

Results: A total of 3,250 participants aged 60 years and older were included in this study. Our findings suggest that multimorbidity is strongly positively associated with depression, anxiety, and stress symptoms. In addition, the positive relations between multimorbidity and depression, anxiety, and stress symptoms are stronger for older female than male adults.

Conclusion: Old adults with multimorbidity are more likely have depression, anxiety, and stress symptoms. This study offers new insight for the mental health from the perspective of multimorbidity among older people, implies that encouraging the accessibility of treatment for multimorbidity in older people with different sex may be effective in promoting mental health in China.
Evolved Features of Artistic Motivation: Analyzing a Brazilian Database Spanning Three Decades
Authors: Varella Marco Antonio Correa
Published: December 2021

Darwin explored the evolutionary processes underlying artistic propensities in humans. He stressed the universality of the human mind by pointing to the shared pleasure which all populations take in dancing, engaging in music, acting, painting, tattooing, and self-decorating. Artistic motivation drives/reinforces individuals to engage in aesthetically oriented activities. As curiosity/play, artistic behavior is hypothesized as a functionally autonomous activity motivated intrinsically through an evolved, specific, and stable aesthetic motivational system. The author tested whether artistic motivation is rather intrinsically sourced, domain-specific, and temporally stable using a large decades-long real-life public Brazilian database of university applications. In Study I, the author analyzed reasons for career-choice responded to by 403,832 late-adolescent applicants (48.84% women), between 1987 and 1998. In Study II, the author analyzed another career-choice reason question responded to by 1,703,916 late-adolescent applicants (51.02% women), between 1987 and 2020. Music, Dance, Scenic Arts, Visual Arts, and Literary Studies, in combination, presented a higher percentage of individuals reporting intrinsic factors (e.g., personal taste/aptitude/fulfillment) and the lower proportion reporting extrinsic motives (e.g., the influence of media/teacher/family, salary, social contribution/prestige) than other career groups. If artistic motivation were a recent by-product of general curiosity or status-seeking, artistic and non-artistic careers would not differ. Overall, intrinsic motives were 2.60–6.35 times higher than extrinsic factors; among artistic applicants’ were 10.81–28.38 times higher, suggesting domain-specificity. Intrinsic motivation did not differ among artistic careers and remained stable throughout the periods. Converging results corroborated a specific, stable, and intrinsically sourced artistic motivation consistent with its possible evolutionary origins.
Influence of the COVID-19 Pandemic on Parenting Stress Across Asian Countries: A Cross-National Study
Authors: Kurata Sawa , Hiraoka Daiki , Ahmad Adlan Aida Syarinaz , Jayanath Subhashini , Hamzah Norhamizan , Ahmad-Fauzi Aishah , Fujisawa Takashi X. , Nishitani Shota , Tomoda Akemi
Published: December 2021

Background: In a previous study, we demonstrated that the accumulation of parenting stress during prolonged school closures and restrictions on daily activities due to the COVID-19 pandemic in Japan indicates the need for mental health intervention for parents at higher risk of parenting stress. However, few studies have focused on parenting stress in other Asian countries, although they have experienced higher numbers of infections. The aim of the present study was to investigate whether parenting stress among caregivers increased across Asia due to school closures and restrictions on activities during the COVID-19 pandemic and to examine whether there were any country-specific, cross-country, or cross-regional risk factors for increased parenting stress.

Methods: We conducted an online survey immediately after the number of new cases in India significantly increased (September–November 2020). We measured parenting stress, anxiety, and fear associated with the COVID-19 crisis, as evaluated by the Parenting Stress Index, Short-Form (PSI-SF), and the Coronavirus Anxiety Scale (CAS), across three Asian countries—India (n = 142), Malaysia (n = 69), and Japan (n = 182)—in addition to the United States (n = 203). We also investigated whether respondents had adverse childhood experiences (ACE) as a risk factor for parenting stress.

Results: For all countries, we found significant increases in participants’ current parenting stress levels, compared to what they recalled regarding their lives before COVID-19-related restrictions and school closures were enacted. Textual analysis qualitatively identified common terms related to parenting stress across all countries. We also found a statistical model that indicated ACE in parents was a critical risk factor for higher parenting stress via increasing anxiety and fear related to the pandemic.

Conclusion: These results indicate the need to improve the mental health of caregivers who are at risk for higher levels of parenting stress during the COVID-19 pandemic in Asian countries as well as Western countries. These results indicate that there is a need to improve the mental health of caregivers who are at risk for higher levels of parenting stress during the COVID-19 pandemic globally.
Quantifying Training and Game Demands of a National Basketball Association Season
Authors: Russell Jennifer L. , McLean Blake D. , Stolp Sean , Strack Donnie , Coutts Aaron J.
Published: December 2021

Purpose: There are currently no data describing combined practice and game load demands throughout a National Basketball Association (NBA) season. The primary objective of this study was to integrate external load data garnered from all on-court activity throughout an NBA season, according to different activity and player characteristics.

Methods: Data from 14 professional male basketball players (mean ± SD; age, 27.3 ± 4.8 years; height, 201.0 ± 7.2 cm; body mass, 104.9 ± 10.6 kg) playing for the same club during the 2017–2018 NBA season were retrospectively analyzed. Game and training data were integrated to create a consolidated external load measure, which was termed integrated load. Players were categorized by years of NBA experience (1-2y, 3-5y, 6-9y, and 10 + y), position (frontcourt and backcourt), and playing rotation status (starter, rotation, and bench).

Results: Total weekly duration was significantly different (p < 0.001) between years of NBA playing experience, with duration highest in 3–5 year players, compared with 6–9 (d = 0.46) and 10+ (d = 0.78) year players. Starters experienced the highest integrated load, compared with bench (d = 0.77) players. There were no significant differences in integrated load or duration between positions.

Conclusion: This is the first study to describe the seasonal training loads of NBA players for an entire season and shows that a most training load is accumulated in non-game activities. This study highlights the need for integrated and unobtrusive training load monitoring, with engagement of all stakeholders to develop well-informed individualized training prescription to optimize preparation of NBA players.
Motivation Levels and Goals for the Practice of Physical Exercise in Five Different Modalities: A Correspondence Analysis
Authors: Borges Juliana Correia , de Oliveira Filho Gilson Gonçalves , de Lira Claudio Andre Barbosa , da Silva Ronaldo Angelo Dias , Alves Eduardo da Silva , Benvenutti Mateus Joacir , Rosa João Paulo Pereira
Published: December 2021

The identification of the practitioner’s profile regarding their motivation level for physical exercise engagement could be a behavioral strategy to increase exercise adherence. The present study investigates the associations between motivation levels, modalities practiced, and goals concerning the practice of physical exercise among physical exercise practitioners. A total of 100 physical exercise practitioners, of which 67 were women, took part in this study. The participants were engaged in extreme fitness program, strength training, fight training, Pilates, and functional training. Motivation level (BREQ-3) and expectations regarding regular physical exercise (IMPRAF-54) were assessed. A multiple correspondence analysis demonstrates preferential relationships between descriptive and non-inferential variables. Strength training and fight training practitioners seek these modalities with the goals of “Health” and “Aesthetics,” demonstrating low autonomy in relation to the behavior for the practice of physical exercise. Extreme conditioning program and functional training practitioners have as goal “Pleasure,” demonstrating medium and high levels of autonomy for such practice and Pilates practitioners have the goal of “Stress Control.” To promote and encourage the regular practice of physical exercise, this strategy could be used to take actions that increase the public’s intention to start or continue in a physical exercise program.
Comparison of General-Social Health and Corona-Induced Anxiety Between Active and Inactive Students in the COVID-19 Pandemic
Authors: Ahmadabadi Somayeh
Published: December 2021

Background: General health includes physical and mental health and their interactions, and physical activity can improve people's mental and social health. The present study has compared general-social health and COVID-19-induced anxiety between active and inactive students during the COVID-19 pandemic.

Methods: A causal-comparative research design was used in this study. The statistical population consisted of all students of the Farhangian University of Mashhad (2,500 students) in 2020, out of whom 752 students were randomly selected (323 men and 429 women). Subjects were assessed for general health, social health, and COVID-19-induced anxiety. Data normality was investigated by the Kolmogorov-Smirnov test and data were analyzed statistically using independent t-test and Pearson correlation coefficient test.

Results: The results of the present study showed that there was a significant difference between general health and COVID-19-induced anxiety of active and inactive students (p = 0.001), but no significant difference was observed between these two groups in social health (p ≥ 0.05). Results obtained regarding the correlation indicated that there was a significant correlation between general and social health in both active and inactive students (p = 0.001). However, there was no significant correlation between COVID-19-induced anxiety and general-social health (p ≥ 0.05).

Conclusion: According to the results of the present study, it can be said that an active lifestyle and physical activity are important factors to improve general health and reduce anxiety, especially in specific conditions of the COVID-19 outbreak. Therefore, it is recommended that students have a regular exercise program to reduce their anxiety and increase the level of their physical health.