bluuebunny/crossref_metadata_2025 huggingface dataset:
Fronto—Parietal Regions Predict Transient Emotional States in Emotion Modulated Response Inhibition via Low Frequency and Beta Oscillations
Authors: Nayak Siddharth , Tsai Arthur C.
Published: June 2022

The current study evaluated the impact of task-relevant emotion on inhibitory control while focusing on midline cortical regions rather than brain asymmetry. Single-trial time-frequency analysis of electroencephalography recordings linked with response execution and response inhibition was done while thirty-four participants performed the emotion modulated stop-signal task. To evaluate individual differences across decision-making processes involved in inhibitory control, a hierarchical drift-diffusion model was used to fit data from Go-trials for each of the 34 participants. Response threshold in the early processing stage for happy and disgust emotions could be distinguished from the later processing stage at the mid-parietal and mid-frontal regions, respectively, by the single-trial power increments in low frequency (delta and theta) bands. Beta desynchronization in the mid-frontal region was specific for differentiating disgust from neutral emotion in the early as well as later processing stages. The findings are interpreted based on the influence of emotional stimuli on early perceptual processing originating as a bottom-up process in the mid-parietal region and later proceeding to the mid-frontal region responsible for cognitive control processing, which resulted in enhanced inhibitory performance. The results show the importance of mid-frontal and mid-parietal regions in single-trial dynamics of inhibitory control processing.
Analyzing the Jacobi Stability of Lü’s Circuit System
Authors: Munteanu Florian
Published: June 2022

By reformulating the circuit system of Lü as a set of two second order differential equations, we investigate the nonlinear dynamics of Lü’s circuit system from the Jacobi stability point of view, using Kosambi–Cartan–Chern geometric theory. We will determine the five KCC invariants, which express the intrinsic geometric properties of the system, including the deviation curvature tensor. Finally, we will obtain necessary and sufficient conditions on the parameters of the system to have the Jacobi stability near the equilibrium points.
Sharp Bounds for Trigonometric and Hyperbolic Functions with Application to Fractional Calculus
Authors: Stojiljković Vuk , Radojević Slobodan , Çetin Eyüp , Čavić Vesna Šešum , Radenović Stojan
Published: June 2022

Sharp bounds for cosh(x)x,sinh(x)x, and sin(x)x were obtained, as well as one new bound for ex+arctan(x)x. A new situation to note about the obtained boundaries is the symmetry in the upper and lower boundary, where the upper boundary differs by a constant from the lower boundary. New consequences of the inequalities were obtained in terms of the Riemann–Liovuille fractional integral and in terms of the standard integral.
Follow-Up Assessment of Intracranial Aneurysms Treated with Endovascular Coiling: Comparison of Compressed Sensing and Parallel Imaging Time-of-Flight Magnetic Resonance Angiography
Authors: Vornetti Gianfranco , Bartiromo Fiorina , Toni Francesco , Dall’Olio Massimo , Cirillo Mario , Speier Peter , Princiotta Ciro , Schmidt Michaela , Tonon Caterina , Zacà Domenico , Lodi Raffaele , Cirillo Luigi
Published: June 2022

The aim of our study was to compare compressed sensing (CS) time-of-flight (TOF) magnetic resonance angiography (MRA) with parallel imaging (PI) TOF MRA in the evaluation of patients with intracranial aneurysms treated with coil embolization or stent-assisted coiling. We enrolled 22 patients who underwent follow-up imaging after intracranial aneurysm coil embolization. All patients underwent both PI TOF and CS TOF MRA during the same examination. Image evaluation aimed to compare the performance of CS to PI TOF MRA in determining the degree of aneurysm occlusion, as well as the depiction of parent vessel and vessels adjacent to the aneurysm dome. The reference standard for the evaluation of aneurysm occlusion was PI TOF MRA. The inter-modality agreement between CS and PI TOF MRA in the evaluation of aneurysm occlusion was almost perfect (κ  =  0.98, p  <  0.001) and the overall inter-rater agreement was substantial (κ  =  0.70, p  <  0.001). The visualization of aneurysm parent vessel in CS TOF images compared with PI TOF images was evaluated to be better in 11.4%, equal in 86.4%, and worse in 2.3%. CS TOF MRA, with almost 70% scan time reduction with respect to PI TOF MRA, yields comparable results for assessing the occlusion status of coiled intracranial aneurysms. Short scan times increase patient comfort, reduce the risk of motion artifacts, and increase patient throughput, with a resulting reduction in costs. CS TOF MRA may therefore be a potential replacement for PI TOF MRA as a first-line follow-up examination in patients with intracranial aneurysms treated with coil embolization.
Phthalate Exposure: From Quantification to Risk Assessment
Authors: Caporossi Lidia , Marino Maria
Published: June 2022

Phthalates (di-esters of phthalic acid) are a group of synthetic organic compounds present in the environment because of their wide use in a multitude of important industrial products (e [...]
Worldwide Evaluation of CAMS-EGG4 CO2 Data Re-Analysis at the Surface Level
Authors: Custódio Danilo , Borrego Carlos , Relvas Hélder
Published: June 2022

This study systematically examines the global uncertainties and biases in the carbon dioxide (CO2) mixing ratio provided by the Copernicus Atmosphere Monitoring Service (CAMS). The global greenhouse gas re-analysis (EGG4) data product from the European Centre for Medium-Range Weather Forecasts (ECMWF) was evaluated against ground-based in situ measurements from more than 160 of stations across the world. The evaluation shows that CO2 re-analysis can capture the general features in the tracer distributions, including the CO2 seasonal cycle and its strength at different latitudes, as well as the global CO2 trend. The emissions and natural fluxes of CO2 at the surface are evaluated on a wide range of scales, from diurnal to interannual. The results highlight re-analysis compliance, reproducing biogenic fluxes as well the observed CO2 patterns in remote environments. CAMS consistently reproduces observations at marine and remote regions with low CO2 fluxes and smooth variability. However, the model’s weaknesses were observed in continental areas, regions with complex sources, transport circulations and large CO2 fluxes. A strong variation in the accuracy and bias are displayed among those stations with different flux profiles, with the largest uncertainties in the continental regions with high CO2 anthropogenic fluxes. Displaying biased estimation and root-mean-square error (RMSE) ranging from values below one ppmv up to 70 ppmv, the results reveal a poor response from re-analysis to high CO2 mixing ratio, showing larger uncertainty of the product in the boundaries where the CAMS system misses solving sharp flux variability. The mismatch at regions with high fluxes of anthropogenic emission indicate large uncertainties in inventories and constrained physical parameterizations in the CO2 at boundary conditions. The current study provides a broad uncertainty assessment for the CAMS CO2 product worldwide, suggesting deficiencies and methods that can be used in the future to overcome failures and uncertainties in regional CO2 mixing ratio and flux estimates.
Significant Mobility of Novel Heteroaggregates of Montmorillonite Microparticles with Nanoscale Zerovalent Irons in Saturated Porous Media
Authors: Shen Chongyang , Teng Jinan , Zheng Wenjuan , Liu Dong , Ma Ke
Published: June 2022

This study conducted laboratory column experiments to systematically examine the transport of novel heteroaggregates of montmorillonite (Mt) microparticles with nanoscale zerovalent irons (nZVIs) in saturated sand at solution ionic strengths (ISs) ranging from 0.001 to 0.2 M. Spherical nZVIs were synthesized using the liquid phase reduction method and were attached on the plate-shaped Mt surfaces in monolayer. While complete deposition occurred for nZVIs in sand, significant transport was observed for Mt-nZVI heteroaggregates at IS ≤ 0.01 M despite the transport decrease with an increasing loading concentration of nZVIs on Mt. The increased mobility of Mt-nZVI heteroaggregates was because the attractions between nZVIs and sand collectors were reduced by the electrostatic repulsions between the Mt and the collector surfaces, which led to a decreased deposition in the sand columns. Complete deposition occurred for the Mt-nZVI heteroaggregates at IS ≥ 0.1 M due to a favorable deposition at Derjaguin–Landau–Verwey–Overbeek (DLVO) primary energy minima. Interestingly, a large fraction of the deposited heteroaggregates was released by reducing IS because of a monotonic decrease of interaction energy with separation distance for the heteroaggregates at low ISs (resulting in repulsive forces), in contrast to the irreversible deposition of nZVIs. Therefore, the fabricated heteroaggregates could also have high mobility in subsurfaces with saline pore water through continuous capture and release using multiple injections of water with low ISs. Our study was the first to examine the transport of heteroaggregates of a plate-like particle with spherical nanoparticles in porous media; the results have important implications in the use of nanoscale zerovalent iron for in situ soil and groundwater remediation.
Exposure Routes and Health Risks Associated with Pesticide Application
Authors: Tudi Muyesaier , Li Hairong , Li Hongying , Wang Li , Lyu Jia , Yang Linsheng , Tong Shuangmei , Yu Qiming Jimmy , Ruan Huada Daniel , Atabila Albert , Phung Dung Tri , Sadler Ross , Connell Des
Published: June 2022

Pesticides play an important role in agricultural development. However, pesticide application can result in both acute and chronic human toxicities, and the adverse effects of pesticides on the environment and human health remain a serious problem. There is therefore a need to discuss the application methods for pesticides, the routes of pesticide exposure, and the health risks posed by pesticide application. The health problems related to pesticide application and exposure in developing countries are of particular concern. The purpose of this paper is to provide scientific information for policymakers in order to allow the development of proper pesticide application technics and methods to minimize pesticide exposure and the adverse health effects on both applicators and communities. Studies indicate that there are four main pesticide application methods, including hydraulic spraying, backpack spraying, basal trunk spraying, and aerial spraying. Pesticide application methods are mainly selected by considering the habits of target pests, the characteristics of target sites, and the properties of pesticides. Humans are directly exposed to pesticides in occupational, agricultural, and household activities and are indirectly exposed to pesticides via environmental media, including air, water, soil, and food. Human exposure to pesticides occurs mainly through dermal, oral, and respiratory routes. People who are directly and/or indirectly exposed to pesticides may contract acute toxicity effects and chronic diseases. Although no segment of the general population is completely protected against exposure to pesticides and their potentially serious health effects, a disproportionate burden is shouldered by people in developing countries. Both deterministic and probabilistic human health risk assessments have their advantages and disadvantages and both types of methods should be comprehensively implemented in research on exposure and human health risk assessment. Equipment for appropriate pesticide application is important for application efficiency to minimize the loss of spray solution as well as reduce pesticide residuals in the environment and adverse human health effects due to over-spraying and residues. Policymakers should implement various useful measures, such as integrated pest management (IPM) laws that prohibit the use of pesticides with high risks and the development of a national implementation plan (NIP) to reduce the adverse effects of pesticides on the environment and on human health.
Investigation of Weather Triggers Preceding Outbreaks of Acute Bovine Liver Disease in Australia
Authors: Manthorpe Eve M. , Rawlin Grant T. , Stevenson Mark A. , Woolford Lucy , Caraguel Charles G. B.
Published: June 2022

Acute bovine liver disease (ABLD) is a hepatic disease affecting cattle sporadically in southern Australia, characterised histologically by striking periportal hepatocellular necrosis. The cause of ABLD is unknown; however, the seasonality and acute presentation of outbreaks suggest mycotoxin involvement. We described the geographical and seasonal occurrence of ABLD reports from 2010 to 2020 in Victoria, Australia, and explored potential weather triggers preceding 26 outbreaks occurring across 23 properties using a case-crossover design. Outbreaks occurred most frequently in autumn/early winter and in herds located along the southern coastal plain of Victoria, and occasionally within the low-lying regions of the Great Dividing Range. Lactating adult dairy cattle represented the most reported cases. We observed a significant association between an increase in average daily dewpoint in the 15 days preceding an ABLD outbreak, suggesting that dew formation may be a key determinant for this disease. Our findings support the etiology of a potent hepatotoxic agent that requires moisture for proliferation and/or toxin production.
Incobotulinum Toxin Type A for Treatment of Ultraviolet-B-Induced Hyperpigmentation: A Prospective, Randomized, Controlled Trial
Authors: Vachiramon Vasanop , Anuntrangsee Tanaporn , Palakornkitti Pasita , Jurairattanaporn Natthachat , Harnchoowong Sarawin
Published: June 2022

Incobotulinum toxin A (IncoBoNT-A) is effective in preventing ultraviolet B (UVB)-induced hyperpigmentation. This prospective, randomized, controlled study aimed to evaluate the effect of IncoBoNT-A on the treatment of UVB-induced hyperpigmentation in 15 volunteers. Five hyperpigmentation squares (2 × 2 cm) were induced by local UVB on the abdomen at baseline. At Day 7, each site was randomized to receive no treatment (control), normal saline, or intradermal IncoBoNT-A injection with 1:2.5, 1:5, and 1:7.5 dilutions (12, 6, and 4 units, respectively). The mean lightness index (L*), hyperpigmentation improvement score evaluated by blinded dermatologists, and participant satisfaction scores were obtained at Days 21, 28, and 35. At Day 21, improvements in mean L* of 1:2.5, 1:5, and 1:7.5 IncoBoNT-A-treated, saline-treated, and control sites were 14.30%, 12.28%, 6.62%, 0.32%, and 4.98%, respectively (p = 0.86). At Day 28, the improvement in mean L* in IncoBoNT-A-treated groups was superior to that in the other groups. In terms of the hyperpigmentation improvement score, 12 participants (80%) experienced better outcomes with the IncoBoNT-A-injected site compared with the other sites. IncoBoNT-A, especially at higher concentrations, showed some positive effects on the treatment of UVB-induced hyperpigmentation. This may serve as an adjuvant treatment for hyperpigmentary conditions that are aggravated by UVB.
Numerical Simulation of Ionospheric Disturbances Due to Rocket Plume and Its Influence on HF Radio Waves Propagation
Authors: Gong Hongwei , Fang Hanxian , Li Zeyun
Published: June 2022

In this paper, the ionospheric disturbances of CO2, which is released by rocket exhaust plumes, was simulated. The effect of this disturbance on the propagation of high-frequency (HF) radio waves at different incident frequencies was also simulated by using three-dimensional digital ray tracing technique. The results show that CO2 can effectively dissipate the background electrons and form ionospheric holes after being released in the ionosphere. At the peak height of ionospheric electron density (about 300 km), the electrons are dissipated fastest and the radius of ionospheric hole is also largest. This is due to the fact that the diffusion coefficient of CO2 usually increases with height while the electron density just increases before reaching its peak height and then decreases with height, and the chemical reaction rate between ions and CO2 also becomes largest at the peak height of electron density (about 300 km). Around 100 s after the release of CO2, when the radio waves at a frequency of 8 MHz pass through the ionosphere with an elevation range of 85~95°, the “secondary focusing effect” can occur, and we believe that this is due to the reflection of HF shortwaves on the walls of the ionospheric holes. With time going on, this phenomenon disappears at 300 s and only one focus is left at this time. For the HF shortwaves at same incident frequency, the focusing effect of waves displays a weakening trend with time increasing, and the height of focus center also ascends gradually. At the same time after CO2 releasing, with the increasing of radio waves frequency, the focusing effect also becomes weaker and the focus center displays an ascending trend.
Explaining the ‘Outliers’ Track in Black Hole X-ray Binaries with a BZ-Jet and Inner-Disk Coupling
Authors: Chang Ning , Liu Xiang , Xie Fu-Guo , Cui Lang , Shan Hao
Published: June 2022

In this paper, we investigate the black hole (BH) spin contribution to jet power, especially for the magnetic arrested disk (MAD), where only inner accretion disk luminosity is closely coupled with the spin-jet power, and try to explain the ‘outliers’ track of the radio LR to X-ray luminosity LX in two black hole X-ray binaries (BHXBs). Our results suggest that the BZ-jet and the inner-disk coupling could account for the ‘outliers’ track of the radio/X-ray correlation in two BHXBs, H1743-322 and MAXI J1348-630. Although the accretion disk of H1743-322 in the outburst could be in the MAD state, there is a lower probability that MAXI J1348-630 is in the MAD state due to its low jet production efficiency. The difference in the inner-disk bolometric luminosity ratio of the two sources implies that these two BHXBs are in different inner-disk accretion states. We further investigate the phase-changing regime of MAXI J1348-630 and find that there is a phase transition around LX/LEdd∼10−3. The assumption of sub-MAD is discussed as well.
Review on Stochastic Approach to Inflation
Authors: Cruces Diego
Published: June 2022

We present a review on the state-of-the-art of the mathematical framework known as stochastic inflation, paying special attention to its derivation, and giving references for the readers interested in results coming from the application of the stochastic framework to different inflationary scenarios, especially to those of interest for primordial black hole formation. During the derivation of the stochastic formalism, we will emphasise two aspects in particular: the difference between the separate universe approach and the true long wavelength limit of scalar inhomogeneities and the generically non-Markovian nature of the noises that appear in the stochastic equations.
A Novel Class of HIV-1 Inhibitors Targeting the Vpr-Induced G2-Arrest in Macrophages by New Yeast- and Cell-Based High-Throughput Screening
Authors: Sato Hirotaka , Murakami Tomoyuki , Matsuura Ryosuke , Abe Masako , Matsuoka Seiji , Yashiroda Yoko , Yoshida Minoru , Akari Hirofumi , Nagasawa Yosuke , Takei Masami , Aida Yoko
Published: June 2022

The human immunodeficiency virus type 1 (HIV-1) accessory protein, Vpr, arrests the cell cycle of the G2 phase, and this Vpr-mediated G2 arrest is implicated in an efficient HIV-1 spread in monocyte-derived macrophages. Here, we screened new candidates for Vpr-targeting HIV-1 inhibitors by using fission yeast- and mammalian cell-based high-throughput screening. First, fission yeast strains expressing the HIV-1 Vpr protein were generated and then treated for 48 h with 20 μM of a synthetic library, including 140,000 chemical compounds. We identified 268 compounds that recovered the growth of Vpr-overexpressing yeast. The selected compounds were then tested in mammalian cells, and those displaying high cytotoxicity were excluded from further cell cycle analysis and imaging-based screening. A flow cytometry analysis confirmed that seven compounds recovered from the Vpr-induced G2 arrest. The cell toxicity and inhibitory effect of HIV-1 replication in human monocyte-derived macrophages (MDM) were examined, and three independent structural compounds, VTD227, VTD232, and VTD263, were able to inhibit HIV-1 replication in MDM. Furthermore, we showed that VTD227, but not VTD232 and VTD263, can directly bind to Vpr. Our results indicate that three new compounds and their derivatives represent new drugs targeting HIV-1 replication and can be potentially used in clinics to improve the current antiretroviral therapy.
Palmitoylation Is Indispensable for Remorin to Restrict Tobacco Mosaic Virus Cell-to-Cell Movement in Nicotiana benthamiana
Authors: Ma Tingting , Fu Shuai , Wang Kun , Wang Yaqin , Wu Jianxiang , Zhou Xueping
Published: June 2022

Remorin (REM) is a plant-specific plasma membrane-associated protein regulating plasmodesmata plasticity and restricting viral cell-to-cell movement. Here, we show that palmitoylation is broadly present in group 1 remorin proteins in Nicotiana benthamiana and is crucial for plasma membrane localization and accumulation. By screening the four members of N. benthamiana group 1 remorin proteins, we found that only NbREM1.5 could significantly hamper tobacco mosaic virus (TMV) cell-to-cell movement. We further showed that NbREM1.5 interacts with the movement protein of TMV in vivo and interferes with its function of expanding the plasmodesmata size exclusion limit. We also demonstrated that palmitoylation is indispensable for NbREM1.5 to hamper plasmodesmata permeability and inhibit TMV cell-to-cell movement.
Human Intramuscular Hyperimmune Gamma Globulin (hIHGG) Anti-SARS-CoV-2—Characteristics of Intermediates and Final Product
Authors: Lachert Elzbieta , Lasocka Joanna , Bielawski Artur , Sulkowska Ewa , Guz Katarzyna , Pyrc Krzysztof , Dabrowska Agnieszka , Wawryniuk-Malmon Agata , Letowska Magdalena , Tomasiewicz Krzysztof , Grabarczyk Piotr
Published: June 2022

This study aims to characterize the intermediates, and the final product (FP) obtained during the production of human intramuscular hyperimmune gamma globulin anti-SARS-CoV-2 (hIHGG anti-SARS-CoV-2) and to determine its stability. Material and methods: hIHGG anti-SARS-CoV-2 was fractionated from 270 convalescent plasma donations with the Cohn method. Prior to fractionation, the plasma was inactivated (Theraflex MB Plasma). Samples were defined using enzyme immunoassays (EIA) for anti-S1, anti-RBD S1, and anti-N antibodies, and neutralization assays with SARS-CoV-2 (VN) and pseudoviruses (PVN, decorated with SARS-CoV-2 S protein). Results were expressed as a titer (EIA) or 50% of the neutralization titer (IC50) estimated in a four-parameter nonlinear regression model. Results: Concentration of anti-S1 antibodies in plasma was similar before and after inactivation. Following fractionation, the anti-S1, anti-RBD, and anti-N (total tests) titers in FP were concentrated approximately 15-fold from 1:4 to 1:63 (1800 BAU/mL), 7-fold from 1:111 to 1:802 and from 1:13 to 1:88, respectively. During production, the IgA (anti-S1) antibody titer was reduced to an undetectable level and the IgM (anti-RBD) titer from 1:115 to 1:24. The neutralizing antibodies (nAb) titer increased in both VN (from 1:40 to 1:160) and PVN (IC50 from 63 to 313). The concentration of specific IgG in the FP did not change significantly for 14 months. Conclusions: The hIHGG anti-SARS-CoV-2 was stable, with concentration up to approximately 15-fold nAb compared to the source plasma pool.
Nuances of Responses to Two Sources of Grapevine Leafroll Disease on Pinot Noir Grown in the Field for 17 Years
Authors: Reynard Jean-Sébastien , Brodard Justine , Zufferey Vivian , Rienth Markus , Gugerli Paul , Schumpp Olivier , Blouin Arnaud G.
Published: June 2022

Grapevine leafroll disease (GLD) is one of the most economically damaging virus diseases in grapevine, with grapevine leafroll-associated virus 1 (GLRaV-1) and grapevine leafroll-associated virus 3 (GLRaV-3) as the main contributors. This study complements a previously published transcriptomic analysis and compared the impact of two different forms of GLD to a symptomless control treatment: a mildly symptomatic form infected with GLRaV-1 and a severe form with exceptionally early leafroll symptoms (up to six weeks before veraison) infected with GLRaV-1 and GLRaV-3. Vine physiology and fruit composition in 17-year-old Pinot noir vines were measured and a gradient of vigor, yield, and berry quality (sugar content and berry weight) was observed between treatments. Virome composition, confirmed by individual RT-PCR, was compared with biological indexing. Three divergent viromes were recovered, containing between four to seven viruses and two viroids. They included the first detection of grapevine asteroid mosaic-associated virus in Switzerland. This virus did not cause obvious symptoms on the indicators used in biological indexing. Moreover, the presence of grapevine virus B (GVB) did not cause the expected corky bark symptoms on the indicators, thus underlining the important limitations of the biological indexing. Transmission of GLRaV-3 alone or in combination with GVB by Planococcus comstocki mealybug did not reproduce the strong symptoms observed on the donor plant infected with a severe form of GLD. This result raises questions about the contribution of each virus to the symptomatology of the plant.
Detection of Ancient Viruses and Long-Term Viral Evolution
Authors: Nishimura Luca , Fujito Naoko , Sugimoto Ryota , Inoue Ituro
Published: June 2022

The COVID-19 outbreak has reminded us of the importance of viral evolutionary studies as regards comprehending complex viral evolution and preventing future pandemics. A unique approach to understanding viral evolution is the use of ancient viral genomes. Ancient viruses are detectable in various archaeological remains, including ancient people’s skeletons and mummified tissues. Those specimens have preserved ancient viral DNA and RNA, which have been vigorously analyzed in the last few decades thanks to the development of sequencing technologies. Reconstructed ancient pathogenic viral genomes have been utilized to estimate the past pandemics of pathogenic viruses within the ancient human population and long-term evolutionary events. Recent studies revealed the existence of non-pathogenic viral genomes in ancient people’s bodies. These ancient non-pathogenic viruses might be informative for inferring their relationships with ancient people’s diets and lifestyles. Here, we reviewed the past and ongoing studies on ancient pathogenic and non-pathogenic viruses and the usage of ancient viral genomes to understand their long-term viral evolution.
Appraisal of COVID-19 Vaccination Acceptance in the Romanian Pregnant Population
Authors: Citu Cosmin , Chiriac Veronica Daniela , Citu Ioana Mihaela , Gorun Oana Maria , Burlea Bogdan , Bratosin Felix , Popescu Daniela-Eugenia , Ratiu Adrian , Buca Oana , Gorun Florin
Published: June 2022

Widespread COVID-19 vaccination is crucial for limiting the spread of SARS-CoV-2 and minimizing the risk of novel variants arising in the general population, especially in pregnant women. According to the publicly available research data, vaccination intentions vary significantly by country, with Romania among the European countries with the lowest vaccination rates. Thus, we sought to determine the scale of acceptance of the COVID-19 vaccination campaign among pregnant women in Romania, as well as the variables affecting their choices. A cross-sectional study was conducted on pregnant women referred to the Obstetrics and Gynecology Clinic of the Timisoara Municipal Emergency Hospital in Romania, where participants were asked to complete an online survey including standardized and unstandardized questionnaires indicating their willingness to receive a COVID-19 vaccine and the reasons for their willingness. Out of the 500 women who were requested to participate, there was a total of 345 validated questionnaires, with 184 vaccinated and 161 unvaccinated pregnant women. The statistically significant determinant factors for COVID-19 vaccination acceptance were the urban area of residence (OR = 0.86), having a higher level of education (OR = 0.81), the third trimester of pregnancy (OR = 0.54), trusting the government (OR = 0.83), being a frequent traveler (OR = 0.76), fearing the severity of COVID-19 (OR = 0.68), the higher availability of COVID-19 vaccines nearby (OR = 0.87), and seeing more people getting vaccinated (OR = 0.75). As there are no increased risks associated with SARS-CoV-2 immunization in pregnant women, the variables identified in this research are crucial in determining the acceptability of COVID-19 vaccines that should be addressed in this vulnerable group to increase vaccination rates.
Immunogenicity Evaluating of the Multivalent COVID-19 Inactivated Vaccine against the SARS-CoV-2 Variants
Authors: Zhang Yuntao , Tan Wenjie , Lou Zhiyong , Huang Baoying , Zhou Weimin , Zhao Yuxiu , Zhang Jin , Liang Hongyang , Li Na , Zhu Xiujuan , Ding Ling , Guo Yancen , He Zhenyu , He Yao , Wang Zhanhui , Ma Bo , Ma Meng , Zhao Suhua , Chang Zhen , Zhao Xue , Zheng Xiaotong , Wu Guizhen , Wang Hui , Yang Xiaoming
Published: June 2022

It has been reported that the novel coronavirus (COVID-19) has caused more than 286 million cases and 5.4 million deaths to date. Several strategies have been implemented globally, such as social distancing and the development of the vaccines. Several severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants have appeared, such as Alpha, Beta, Gamma, Delta, and Omicron. With the rapid spread of the novel coronavirus and the rapidly changing mutants, the development of a broad-spectrum multivalent vaccine is considered to be the most effective way to defend against the constantly mutating virus. Here, we evaluated the immunogenicity of the multivalent COVID-19 inactivated vaccine. Mice were immunized by multivalent COVID-19 inactivated vaccine, and the neutralizing antibodies in serum were analyzed. The results show that HB02 + Delta + Omicron trivalent vaccine could provide broad spectrum protection against HB02, Beta, Delta, and Omicron virus. Additionally, the different multivalent COVID-19 inactivated vaccines could enhance cellular immunity. Together, our findings suggest that the multivalent COVID-19 inactivated vaccine can provide broad spectrum protection against HB02 and other virus variants in humoral and cellular immunity, providing new ideas for the development of a broad-spectrum COVID-19 vaccine.
Anti-SARS-CoV-2 Vaccination Campaign: Risk Perception, Emotional States, and Vaccine Hesitancy in a Sample of Adolescents’ Vaccinated Parents in Southern Italy
Authors: Savarese Giulia , Carpinelli Luna , De Chiara Anna , Giordano Claudio , Perillo Matilde , Fornino Domenico , De Caro Francesco , Capunzo Mario , Moccia Giuseppina
Published: June 2022

Background: The international strategic plan for COVID-19 vaccines remains the practical option for the protection of health. However, vaccine hesitancy remains an obstacle to full population vaccination, with rapid developments in COVID-19 vaccines and concerns about efficacy acting as influencing factors. Aim: The present study investigated the perception of vaccine hesitancy among parents of adolescents in order to explore the reasons and related emotional states. Methods: In January–March 2022, an online questionnaire was administered to a sample of parents who brought their children to the vaccine center of a local health unit, ASL Salerno (Campania, Italy). Results: The participants were 1105 parents (F = 64.6%; mean age = 47.37 years, SD = 7.52) of adolescents (F = 47.6%; mean age = 14.83 years, SD = 1.72). All parents had received the COVID-19 vaccine. Regarding the vaccination schedule, 46.8% believed that children receive more vaccinations than they should; 25.1% believed that it is better to develop immunity rather than get vaccinated; 41.2% believed that their child could have side effects; 29.6% were very concerned that vaccines were unsafe, while 35.3% believed vaccines do not prevent disease; 21.5% were very reluctant about pediatric vaccines; and 23.8% did not trust the information received. Conclusions: In order to increase vaccination and reduce the prevalence of vaccine hesitancy, it is essential to support the value of vaccination among all parents and make information more accessible and usable through competent pediatricians.
ChAdOx1 nCoV-19 Immunogenicity and Immunological Response Following COVID-19 Infection in Patients Receiving Maintenance Hemodialysis
Authors: Prasithsirikul Wisit , Nopsopon Tanawin , Phutrakool Phanupong , Suwanwattana Pawita , Kantagowit Piyawat , Pongpirul Wannarat , Jongkaewwattana Anan , Pongpirul Krit
Published: June 2022

Patients with end-stage renal disease (ESRD) receiving hemodialysis (HD) were found to have a decreased immune response following mRNA COVID-19 immunization. ChAdOx1 nCoV-19 was a promising COVID-19 vaccine that performed well in the general population, but the evidence on immunogenicity in ESRD with HD patients was limited. Moreover, the immunological response to COVID-19 infection was inconclusive in patients with ESRD and HD. The aim of this study was to investigate the immunogenicity of ChAdOx1 nCoV-19 vaccination and the immunological response after COVID-19 infection in ESRD patients with HD. The blood samples were obtained at baseline, 1-month, and 3-month follow-up after each shot or recovery. All participants were measured for anti-spike IgG by the ELISA method, using Euroimmun. This study found a significant increase in anti-spike IgG after 1 month of two-shot ChAdOx1 nCoV-19 vaccination, followed by a significant decrease after 3 months. On the other hand, the anti-spike IgG was maintained in the post-recovery group. There was no significant difference in the change of anti-spike IgG between the one-shot ChAdOx1 nCoV-19-vaccinated and post-recovery groups for both 1-month and 3-month follow-ups. The seroconversion rate for the vaccinated group was 60.32% at 1 month after one-shot vaccination and slightly dropped to 58.73% at the 3-month follow-up, then was 92.06% at 1 month after two-shot vaccination and reduced to 82.26% at the 3-month follow-up. For the recovered group, the seroconversion rate was 95.65% at 1 month post-recovery and 92.50% at 3-month follow-up. This study demonstrated the immunogenicity of two-dose ChAdOx1 nCoV-19 in ESRD patients with HD for humoral immunity. After COVID-19 infection, the humoral immune response was strong and could be maintained for at least three months.
A Needs-Based Analysis of Teaching on Vaccinations and COVID-19 in German Medical Schools
Authors: Baessler Franziska , Zafar Ali , Mengler Katharina , Natus Ricarda Nadine , Dutt Anne Josephine , Kuhlmann Manuel , Çinkaya Emre , Hennes Simon
Published: June 2022

The COVID-19 pandemic highlights the need for improving public confidence in vaccines. Academic gaps and redundancies on vaccinations must be identified to revise the medical curriculum for up-to-date training of medical students. This cross-sectional survey assessed the status of vaccine-related teaching in general and specific to COVID-19 in medical schools across Germany. A total of 4313 medical students completed a questionnaire comprising items on national learning goals and perceived needs for teaching on vaccinations. Mixed methods were used to analyse data quantitatively for relative frequencies (%) and correlations between teaching items and semesters (Spearman’s rho), and qualitatively (content analysis). Our findings showed that 38.92% of the students were dissatisfied with teaching on vaccine-preventable diseases, but the perceived satisfaction increased in later semesters (r = 0.46, p < 0.001). Moreover, 75.84% and 68.15% of the students were dissatisfied with teaching related to vaccine scepticism and vaccine-related communication strategies, respectively. Furthermore, 63.79% reported dissatisfaction with teaching on COVID-19 disease and 72.93% with teaching on COVID-19 vaccines. A total of 79.12% stated they educated others on COVID-19 and its vaccines and 75.14% felt responsible to do so. A majority of the medical students were dissatisfied with teaching on dealing with vaccine scepticism, communication strategies and COVID-19 vaccines. We recommend practice-oriented vaccine education, especially for teaching communication skills to medical students.
Characterization of Equine Chronic Tendon Lesions in Low- and High-Field Magnetic Resonance Imaging
Authors: Doll Carla Ulrike , von Pueckler Kerstin , Offhaus Julia , Berner Dagmar , Burk Janina
Published: June 2022

In equine medicine, experience regarding MRI of chronic tendon lesions is limited, and evidence on the suitability of different sequences in 3 T high-field MRI is scarce. Therefore, macroscopically healthy and altered tendons were examined by histology and in 0.27 T low- and 3 T high-field MRI, focusing on T1-weighted (T1w) sequences to visualize chronic lesions. In high-field MRI, tendons were positioned parallel (horizontal) and perpendicular (vertical) to the magnetic field, acknowledging the possible impact of the magic angle effect. The images were evaluated qualitatively and signal intensities were measured for quantitative analysis. Qualitative evaluation was consistent with the quantitative results, yet there were differences in lesion detection between the sequences. The low-field T1w GRE sequence and high-field T1w FLASH sequence with vertically positioned tendons displayed all tendon lesions. However, the horizontally scanned high-field T1w SE sequence failed to detect chronic tendon lesions. The agreement regarding tendon signal intensities was higher between high-field sequences scanned in the same orientation (horizontal or vertical) than between the same types of sequence (SE or FLASH), demonstrating the impact of tendon positioning. Vertical scanning was superior for diagnosis of the tendon lesions, suggesting that the magic angle effect plays a major role in detecting chronic tendon disease.
Antibacterial Activity of Romanian Propolis against Staphylococcus aureus Isolated from Dogs with Superficial Pyoderma: In Vitro Test
Authors: Dégi János , Herman Viorel , Igna Violeta , Dégi Diana Maria , Hulea Anca , Muselin Florin , Cristina Romeo Teodor
Published: June 2022

Staphylococcal infection treatment in dogs is frequently associated with adverse side effects, high costs, prolonged treatment, and resistant strain selection. Staphylococcus aureus is the most frequently isolated staphylococci in cases of canine superficial pyoderma. The number of Staphylococcus strains to exhibit primary resistance to various drugs in vitro is increasing. Propolis has a diverse chemical composition and well-known therapeutic properties against bacterial infections. The current investigation evaluated in vitro the antimicrobial activity of the commercial allopathic antimicrobials, Romanian propolis ethanolic extracts, against clinical Staphylococcus aureus strains isolated from superficial dermatitis clinical samples in dogs and two reference strains: Staphylococcus aureus ATCC 25923 and Staphylococcus aureus ATCC 43300, as the MSSA and MRSA positive controls, respectively, in western Romania. We used the microdilution broth technique to evaluate the susceptibility profile of the bacteria. The minimum inhibitory concentration (MIC) of the Romanian propolis ethanolic extract ranged from 6 to 10 μg/mL for all isolates, determined by the broth microdilution method. The MICs of ethanolic Romanian propolis extracts had a pronounced antibacterial activity. These results indicate that propolis can potentially be used and recommended for in vivo experiments as a promising therapeutic agent against Staphylococcus aureus infections in superficial dermatitis of dogs.
Use of Multi-Criteria Decision Analysis (MCDA) for Mapping Erosion Potential in Gulf of Mexico Watersheds
Authors: Cartwright John H. , Shammi Sadia Alam , Rodgers John C.
Published: June 2022

The evaluation of soil erosion is often assessed using traditional soil-loss models such as the Revised Universal Soil-Loss Equation (RUSLE) and the Soil and Water Assessment Tool (SWAT). These models provide quantitative outputs for sediment yield and are often integrated with geographic information systems (GIS). The work described here is focused on transitioning towards a qualitative assessment of erosion potential using Multi-Criteria Decision Analysis (MCDA), for improved decision-support and watershed-management prioritization in a northern Gulf of Mexico coastal watershed. The foundation of this work conceptually defined watershed erosion potential based on terrain slope, geomorphology, land cover, and soil erodibility (as defined by the soil K-factor) with precipitation as a driver. These criteria were evaluated using a weighted linear combination (WLC) model to map generalized erosion potential. The sensitivity of individual criteria was accessed with the one-at-a-time (OAT) method, which simply removed one criterion and re-evaluated erosion potential. The soil erodibility and slope were found to have the most influence on erosion-potential modeling. Expert input was added through MCDA using the Analytical Hierarchy Process (AHP). The AHP allows for experts to rank criteria, providing a quantitative metric (weight) for the qualitative data. The individual AHP weights were altered in one-percent increments to help identify areas of alignment or commonality in erosion potential across the drainage basin. These areas were used to identify outliers and to develop an analysis mask for watershed management area prioritization. A comparison of the WLC, AHP, ensembled model (average of WLC and AHP models), and SWAT output data resulted in visual geographic alignment between the WLC and AHP erosion-potential output with the SWAT sediment-yield output. These observations yielded similar results between the qualitative and quantitative erosion-potential assessment approaches, with alignment in the upper and lower ranks of the mapped erosion potentials and sediment yields. The MCDA, using the AHP and ensembled modeling for mapping watershed potential, provided the advantage of more quickly mapping erosion potential in coastal watersheds for improved management of the environmental resources linked to erosion.
Econometric Model for the Financial Performance of Romanian Companies Operating in the Water Supply and Sewerage Field
Authors: Topor Dan Ioan , Anghelache Constantin , Ionescu Constantin Aurelian , Căpușneanu Sorinel , Fülöp Melinda Timea , Cioca Ionela Cornelia , Rakoș Ileana-Sorina , Coman Mihaela Denisa , Breaz Teodora Odett , Bakó (Fülöp) Kinga-Erzsébet
Published: June 2022

This research aims to measure the financial performance of companies in the water and sewerage sector by creating a sustainable econometric model for making long-term strategic decisions for managers and stakeholders. The research methodology consisted of the use and statistical processing of the data included in the summary financial statements of 40 regional operators in the field from 2014 to 2020. Multiple linear regression has been created with which stakeholders and water and sewerage specialists can shape changes in value-added variation, the average cost per employee, labor productivity, and energy expenditure on the net profit of water operators and sewerage. The results indicated that the independent variables used, such as value-added, labor productivity, or intangible assets have a direct influence on increasing the net profit of water and sewerage companies. Other independent variables such as the average cost per employee or the expenditure on electricity and water negatively influence the increase in the net profit of companies in the water and sewerage sector. The conclusions indicated that the average net profit is influenced by independent variables and the model created, and it can be successfully applied to other international companies in the field.
Effects of Calcium on the Removal of Ammonium from Aged Landfill Leachate by Struvite Precipitation
Authors: Rayshouni Hussein , Wazne Mahmoud
Published: June 2022

Ammonium in landfill leachates is a major contributor to environmental degradation if not effectively treated. However, it could be converted to a valuable fertilizer when it is co-precipitated with phosphate and magnesium as struvite. Low-cost magnesium and phosphate sources are sought to offset the co-precipitation treatment costs, but most of the identified alternative magnesium sources have significant amounts of calcium, which may negatively impact the ammonium removal rates. In this study, the effects of calcium on ammonium removal from high-strength aged field landfill leachate as struvite were investigated. Laboratory-scale batch tests were conducted to assess the effects of the pH, Mg2+:NH4+:PO43−, and Ca2+:Mg2+ molar ratios on ammonium removal. Magnesium chloride salt was used as a model dissolved magnesium source, whereas different compounds derived from dolomite (CaMg(CO3)2) were used as model solid-phase magnesium sources. X-ray powder diffraction and activity ratio diagrams were used to delineate the ammonium removal mechanisms and struvite stability. The ammonium removal rate of the magnesium salt decreased from approximately 97% to 70%, upon increasing the Ca2+:Mg2+ molar ratio from 0 to 1.0, for the Mg2+:NH4+:PO43− molar ratio of 1.25:1:1.25 and pH = 9.5. For similar pH values, as well as the Mg2+:NH4+:PO43− and Ca2+:Mg2+ molar ratios, the ammonium removal rates by the dolomite-derived compounds reached up to 55%, which highlighted the limited availability of magnesium in solid phases, in addition to the negative impacts of calcium. The diffractometric analysis and thermodynamic calculations revealed the stable regions of struvite in the presence of competing solid phases. The new findings in this study could aid in the design of ammonium and phosphate removal and recovery systems by struvite precipitation.
Continuous Production of Volatile Fatty Acids (VFAs) from Swine Manure: Determination of Process Conditions, VFAs Composition Distribution and Fermentation Broth Availability Analysis
Authors: Wang Zhiwei , Wang Weiwu , Li Ping , Leng Yaping , Wu Jinhua
Published: June 2022

For pollution control and waste utilization, a promising future direction is to obtain high-value carbon sources from organic waste. In this experiment, swine manure was efficiently converted into high concentration volatile fatty acids through continuous hydrolysis-acidification bioreactors. This study determined the process conditions, the composition distribution of volatile fatty acids and the availability of fermentation broth. The results showed that the reactor with a hydraulic retention time of 1.5 days had the optimal production performance of volatile fatty acids. The highest hydrolysis degree (62.2%) and acidification degree (42.5%) were realized in this reactor at the influent soluble chemical oxygen demand of 5460 mg/L. Furthermore, when the influent soluble chemical oxygen demand was 7660 mg/L, volatile fatty acids of 6065 mg-COD/L could be produced stably, and the proportion of volatile fatty acids in soluble chemical oxygen demand was the largest (75%). Additionally, the fermentation broth rich in volatile fatty acids could be applied to deep nitrogen and phosphorus removal. This work provides a productive approach to resource recovery from swine manure.