bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization and Lifetime Dietary Risk Assessment of Eighteen Pyrrolizidine Alkaloids and Pyrrolizidine Alkaloid N-Oxides in New Zealand Honey
Authors: Pearson Andrew J. , Nicolas Jeane E. F. , Lancaster Jane E. , Symes C. Wymond
Published: December 2021

Pyrrolizidine alkaloids (PAs) are a large group of botanical toxins of concern, as they are considered genotoxic carcinogens, with long-term dietary exposure presenting an elevated risk of liver cancer. PAs can contaminate honey through honeybees visiting the flowers of PA-containing plant species. A program of monitoring New Zealand honey has been undertaken over several years to build a comprehensive dataset on the concentration, regional and seasonal distribution, and botanical origin of 18 PAs and PA N-oxides. A bespoke probabilistic exposure model has then been used to assess the averaged lifetime dietary risk to honey consumers, with exposures at each percentile of the model characterized for risk using a margin of exposure from the Joint World Health Organization and United Nations Food and Agriculture Organization Expert Committee on Food Additives (JECFA) Benchmark Dose. Survey findings identify the typical PA types for New Zealand honey as lycopsamine, echimidine, retrorsine and senecionine. Regional and seasonal variation is evident in the types and levels of total PAs, linked to the ranges and flowering times of certain plants. Over a lifetime basis, the average exposure an individual will receive through honey consumption is considered within tolerable levels, although there are uncertainties over high and brand-loyal consumers, and other dietary contributors. An average lifetime risk to the general population from PAs in honey is not expected. However, given the uncertainties in the assessment, risk management approaches to limit or reduce exposures through honey are still of value.
Delivery of Toxins and Effectors by Bacterial Membrane Vesicles
Authors: Macion Adrian , Wyszyńska Agnieszka , Godlewska Renata
Published: December 2021

Pathogenic bacteria interact with cells of their host via many factors. The surface components, i.e., adhesins, lipoproteins, LPS and glycoconjugates, are particularly important in the initial stages of colonization. They enable adhesion and multiplication, as well as the formation of biofilms. In contrast, virulence factors such as invasins and toxins act quickly to damage host cells, causing tissue destruction and, consequently, organ dysfunction. These proteins must be exported from the bacterium and delivered to the host cell in order to function effectively. Bacteria have developed a number of one- and two-step secretion systems to transport their proteins to target cells. Recently, several authors have postulated the existence of another transport system (sometimes called “secretion system type zero”), which utilizes extracellular structures, namely membrane vesicles (MVs). This review examines the role of MVs as transporters of virulence factors and the interaction of toxin-containing vesicles and other protein effectors with different human cell types. We focus on the unique ability of vesicles to cross the blood–brain barrier and deliver protein effectors from intestinal or oral bacteria to the central nervous system.
New Insights into the Hypotensins from Tityus serrulatus Venom: Pro-Inflammatory and Vasopeptidases Modulation Activities
Authors: Duzzi Bruno , Silva Cristiane Castilho Fernandes , Kodama Roberto Tadashi , Cajado-Carvalho Daniela , Squaiella-Baptistão Carla Cristina , Portaro Fernanda Calheta Vieira
Published: December 2021

The Tityus serrulatus scorpion is considered the most dangerous of the Brazilian fauna due to the severe clinical manifestations in injured victims. Despite being abundant components of the venom, few linear peptides have been characterized so far, such as hypotensins. In vivo studies have demonstrated that hypotensin I (TsHpt-I) exerts hypotensive activity, with an angiotensin-converting enzyme (ACE)-independent mechanism of action. Since experiments have not yet been carried out to analyze the direct interaction of hypotensins with ACE, and to deepen the knowledge about these peptides, hypotensins I and II (TsHpt-II) were studied regarding their modulatory action over the activities of ACE and neprilysin (NEP), which are the peptidases involved in blood pressure control. Aiming to search for indications of possible pro-inflammatory action, hypotensins were also analyzed for their role in murine macrophage viability, the release of interleukins and phagocytic activity. TsHpt-I and -II were used in kinetic studies with the metallopeptidases ACE and NEP, and both hypotensins were able to increase the activity of ACE. TsHpt-I presented itself as an inhibitor of NEP, whereas TsHpt-II showed weak inhibition of the enzyme. The mechanism of inhibition of TsHpt-I in relation to NEP was defined as non-competitive, with an inhibition constant (Ki) of 4.35 μM. Concerning the analysis of cell viability and modulation of interleukin levels and phagocytic activity, BALB/c mice’s naïve macrophages were used, and an increase in TNF production in the presence of TsHpt-I and -II was observed, as well as an increase in IL-6 production in the presence of TsHpt-II only. Both hypotensins were able to increase the phagocytic activity of murine macrophages in vitro. The difference between TsHpt-I and -II is the residue at position 15, with a glutamine in TsHpt-I and a glutamic acid in TsHpt-II. Despite this, kinetic analyzes and cell assays indicated different actions of TsHpt-I and -II. Taken together, these results suggest a new mechanism for the hypotensive effects of TsHpt-I and -II. Furthermore, the release of some interleukins also suggests a role for these peptides in the venom inflammatory response. Even though these molecules have been well studied, the present results suggest a new mechanism for the hypotensive effects of TsHpt-I
Neglected Venomous Animals and Toxins: Underrated Biotechnological Tools in Drug Development
Authors: Coelho Guilherme Rabelo , da Silva Daiane Laise , Beraldo-Neto Emidio , Vigerelli Hugo , de Oliveira Laudiceia Alves , Sciani Juliana Mozer , Pimenta Daniel Carvalho
Published: December 2021

Among the vast repertoire of animal toxins and venoms selected by nature and evolution, mankind opted to devote its scientific attention—during the last century—to a restricted group of animals, leaving a myriad of toxic creatures aside. There are several underlying and justifiable reasons for this, which include dealing with the public health problems caused by envenoming by such animals. However, these studies became saturated and gave rise to a whole group of animals that become neglected regarding their venoms and secretions. This repertoire of unexplored toxins and venoms bears biotechnological potential, including the development of new technologies, therapeutic agents and diagnostic tools and must, therefore, be assessed. In this review, we will approach such topics through an interconnected historical and scientific perspective that will bring up the major discoveries and innovations in toxinology, achieved by researchers from the Butantan Institute and others, and describe some of the major research outcomes from the study of these neglected animals.
Towards a New μ→eγ Search with the MEG II Experiment: From Design to Commissioning
Authors: Chiappini Marco , Francesconi Marco , Kobayashi Satoru , Meucci Manuel , Onda Rina , Schwendimann Patrick ,   on behalf of the MEG II Collaboration
Published: December 2021

The MEG experiment represents the state of the art in the search for the Charged Lepton Flavour Violating μ+→e+γ decay. With its first phase of operations at the Paul Scherrer Institut (PSI), MEG set the most stringent upper limit on the BR (μ+→e+γ)≤4.2×10−13 at 90% confidence level, imposing one of the tightest constraints on models predicting LFV-enhancements through new physics beyond the Standard Model. An upgrade of the MEG experiment, MEG II, was designed and it is presently in the commissioning phase, aiming at a sensitivity level of 6×10−14. The MEG II experiment relies on a series of upgrades, which include an improvement of the photon detector resolutions, brand new detectors on the positron side with better acceptance, efficiency and performances and new and optimized trigger and DAQ electronics to exploit a muon beam intensity twice as high as that of MEG (7×107 μ+/s). This paper presents a complete overview of the MEG II experimental apparatus and the current status of the detector commissioning in view of the physics data taking in the upcoming three years.
Serum Antibody Response Comparison and Adverse Reaction Analysis in Healthcare Workers Vaccinated with the BNT162b2 or ChAdOx1 COVID-19 Vaccine
Authors: Lim Sung-Hee , Choi Seong-Hyeok , Kim Bora , Kim Ji-Youn , Ji Young-Sok , Kim Se-Hyung , Kim Chan-Kyu , Kim Tark , Choo Eun-Ju , Moon Ji-Eun , Yun Jina , Park Seong-Kyu
Published: December 2021

The COVID-19 pandemic is changing rapidly and requires different strategies to maintain immunization. In Korea, different COVID-19 vaccines are recommended and available for various populations, including healthcare workers (HCWs) at high risk of SARS-CoV-2 infection. We plan to evaluate the adverse events (AEs) and immunogenicity of the BNT162b2 and ChAdOx1 vaccines in HCWs at a single center. This cohort study included HCWs fully vaccinated with either BNT162b2 or ChAdOx1. Blood samples were taken eight weeks after the second vaccination with both COVID-19 vaccines and six months after the second vaccination from participants with the BNT162b2 vaccine. The primary endpoint for immunogenicity was the serum neutralizing antibody responses eight weeks after vaccination. The secondary endpoint was the incidence of various AEs within 28 days of each vaccination. Between 16 March and 23 June 2021, 115 participants were enrolled (65 in the ChAdOx1 group and 50 in the BNT162b2 group). Significantly higher surrogate virus neutralization test (sVNT) inhibition was observed in participants vaccinated with two doses of BNT162b2 (mean (SD) 91.4 (9.68)%) than in those vaccinated with ChAdOx1 (mean (SD) 73.3 (22.57)%). The effectiveness of the BNT162b2 vaccine was maintained across all age and gender categories. At six months after the second dose, serum antibody levels declined significantly in the BNT162b2 group. The main adverse events, including fever, myalgia, fatigue, and headache, were significantly higher in the ChAdOx1 group after the first dose, whereas, after the second dose, those AEs were significantly higher in the BNT162b2 group (p < 0.05). Two doses of either the ChAdOx1 or the BNT162b2 COVID-19 vaccine resulted in very high seropositivity among the HCWs at our center. The quality of the antibody response, measured by sVNT inhibition, was significantly better with the BNT162b2 vaccine than with the ChAdOx1 vaccine. There was no significant association between neutralizing antibody response and AE after each vaccination in our cohort.
Engineering Antigens to Assemble into Polymer Particle Vaccines for Prevention of Streptococcus suis Infection
Authors: Gonzaga Zennia Jean C. , Chen Shuxiong , Lehoux Mélanie , Segura Mariela , Rehm Bernd H. A.
Published: December 2021

Streptococcus suis is a zoonotic pathogen affecting pigs and humans. This bacterium causes severe economic losses in the swine industry and poses a serious threat to public health and food safety. There is no effective commercial vaccine available for pigs or humans. In this study, we applied the biopolymer particle (BP) vaccine technology to incorporate seven conserved S. suis antigens (38 kDa protein (38), enolase (Enol), SSU1915, SSU1355, SSU0185, SSU1215, and SSU1773 (SSU1 and SSU2)). Two combinations of these antigens (38 and Enol; all SSU antigens designated as SSU1 and SSU2) were engineered to mediate production of BPs coated with either antigens 38 and Enol or SSU1 and SSU2 inside recombinant Escherichia coli. The isolated and purified empty BPs, 38-BP-Enol and SSU1-BP-SSU2, showed size ranges of 312–428 nm and 292–344 nm with and without the QuilA® adjuvant, respectively, and all showed a negative surface charge. Further characterization of purified BPs confirmed the presence of the expected antigen-comprising fusion proteins as assessed by tryptic peptide fingerprinting analysis using quadrupole time-of-flight mass spectrometry and immunoblotting. Vaccination with 38-BP-Enol and SSU1-BP-SSU2 formulated with and without QuilA® adjuvant induced significant antigen-specific humoral immune responses in mice. Antigen-coated BPs induced significant and specific Ig (IgM + IgG) and IgG immune responses (1.0 × 106–1.0 × 107) when compared with mice vaccinated with empty BPs. Functionality of the immune response was confirmed in challenge experiments using an acute murine S. suis infection model, which showed 100% survival of the 38-BP-Enol and SSU1-BP-SSU2 vaccinated mice compared to 70% survival when vaccinated with empty BPs. Overall, our data suggest that S. suis antigen-coated BPs could be developed into particulate vaccines that induce protective immunity against S. suis infections.
Safety and Immunogenicity of M2-Deficient, Single Replication, Live Influenza Vaccine (M2SR) in Adults
Authors: Eiden Joseph , Gordon Gilad , Fierro Carlos , Herber Renee , Aitchison Roger , Belshe Robert , Greenberg Harry , Hoft Daniel , Hatta Yasuko , Moser Michael J. , Tary-Lehmann Magdalena , Kawaoka Yoshihiro , Neumann Gabriele , Radspinner Paul , Bilsel Pamuk
Published: December 2021

M2SR (M2-deficient single replication) is an investigational live intranasal vaccine that protects against multiple influenza A subtypes in influenza-naïve and previously infected ferrets. We conducted a phase 1, first-in-human, randomized, dose-escalation, placebo-controlled study of M2SR safety and immunogenicity. Adult subjects received a single intranasal administration with either placebo or one of three M2SR dose levels (106, 107 or 108 tissue culture infectious dose (TCID50)) expressing hemagglutinin and neuraminidase from A/Brisbane/10/2007 (H3N2) (24 subjects per group). Subjects were evaluated for virus replication, local and systemic reactions, adverse events (AE), and immune responses post-vaccination. Infectious virus was not detected in nasal swabs from vaccinated subjects. At least one AE (most commonly mild nasal rhinorrhea/congestion) was reported among 29%, 58%, and 83% of M2SR subjects administered a low, medium or high dose, respectively, and among 46% of placebo subjects. No subject had fever or a severe reaction to the vaccine. Influenza-specific serum and mucosal antibody responses and B- and T-cell responses were significantly more frequent among vaccinated subjects vs. placebo recipients. The M2SR vaccine was safe and well tolerated and generated dose-dependent durable serum antibody responses against diverse H3N2 influenza strains. M2SR demonstrated a multi-faceted immune response in seronegative and seropositive subjects.
COVID-19 Vaccination in Patients with Pulmonary Arterial Hypertension and Chronic Thromboembolic Pulmonary Hypertension: Safety Profile and Reasons for Opting against Vaccination
Authors: Wieteska-Miłek Maria , Szmit Sebastian , Florczyk Michał , Kuśmierczyk-Droszcz Beata , Ryczek Robert , Kurzyna Marcin
Published: December 2021

The incidence of COVID-19 infection in patients with pulmonary arterial hypertension (PAH) and chronic thromboembolic pulmonary hypertension (CTEPH) is similar to that in the general population, but the mortality rate is much higher. COVID-19 vaccination is strongly recommended for PAH/CTEPH patients. The aim of our cross-sectional study was to identify reasons why PAH/CTEPH patients refused vaccination against COVID-19. Moreover, we assessed the safety profile of approved COVID-19 vaccines in PAH/CTEPH patients. We examined 261 patients (164 PAH patients and 97CTEPH patients) with a median age of 60 (18–92) years, 62% of which were female. Sixty-one patients (23%) refused to be vaccinated. The main reason for unwillingness to be vaccinated was anxiety about adverse events (AEs, 61%). Age and fear of COVID-19 in the univariate analysis and age ≥60 years in the multivariate regression analysis were factors that impacted willingness to be vaccinated (OR = 2.5; p = 0.005). AEs were reported in 61% of vaccinated patients after the first dose and in 40.5% after the second dose (p = 0.01). The most common reported AEs were pain at the injection site (54.5%), fever (22%), fatigue (21%), myalgia (10.5%), and headache (10%). A lower percentage of AEs was reported in older patients (OR = 0.3; p = 0.001). The COVID-19 vaccines are safe for PAH/CTEPH patients. The results obtained in this study may encourage patients of these rare but severe cardio-pulmonary diseases to get vaccinated against COVID-19.
COVID-19 Vaccination Acceptance in China after It Becomes Available: A Cross-Sectional Study
Authors: Zhou Qian , Tian Tian , Ni Jie , Zhao Xiaoheng , Li Hong , Yang Yili , Zhang Yumeng , Pan Jay
Published: December 2021

The outbreak of coronavirus disease 2019 (COVID-19) has led to numerous tragic deaths all over the world. Great efforts have been made by worldwide nations for COVID-19 targeted vaccine development since the disease outbreak. In January 2021, the Chinese government started to provide free vaccination among nationwide communities, which was optional for citizens. As no evidence has been provided so far regarding COVID-19 vaccination acceptance since the initiation of nationwide vaccination, this study aims to investigate COVID-19 vaccination acceptance among Chinese citizens as well as its associated factors as an attempt to bridge such gap embedded in the current literature. An anonymous cross-sectional study was conducted online in March and April 2021 among adults, with the survey questionnaire designed based on the framework of the health belief model (HBM). Information on socio-demographics, risk perception, past pandemic-related experience, awareness of vaccination as well as acceptance of COVID-19 vaccination were collected. Chi-squared test and multi-level regression were performed to distinguish the acceptance between different groups as well as to identify the significant predictors. A total of 3940 participants completed the survey, with 90.6% of the participants reporting strong willingness to get vaccinated. A list of factors were found to be significantly associated with individuals’ acceptance of vaccination, including the region of residence, ethnicity, annual income, whether or not they had experienced a major pandemic event in the past, risk perception of the COVID-19 as well as the awareness of receiving vaccination. Safety concerns about the vaccine (27.7%), concerns about receiving vaccination immediately after newly developed vaccines were released into the market (22.4%) as well as concerns about the potential side effects induced by vaccination (22.1%) were identified as the primary reasons of residents’ resistance against vaccination. Overall, residents demonstrated strong willingness to receive vaccination against COVID-19 in China. However, the improvement of vaccination-related knowledge among Chinese residents should be highlighted as a critical strategy to facilitate the penetration of nationwide vaccination in order to ultimately achieve the establishment of herd immunity in China.
Major Complex Trait for Early De Novo Programming ‘CoV-MAC-TED’ Detected in Human Nasal Epithelial Cells Infected by Two SARS-CoV-2 Variants Is Promising to Help in Designing Therapeutic Strategies
Authors: Costa José Hélio , Aziz Shahid , Noceda Carlos , Arnholdt-Schmitt Birgit
Published: December 2021

Background: Early metabolic reorganization was only recently recognized as an essentially integrated part of immunology. In this context, unbalanced ROS/RNS levels connected to increased aerobic fermentation, which is linked to alpha-tubulin-based cell restructuring and control of cell cycle progression, were identified as a major complex trait for early de novo programming (‘CoV-MAC-TED’) during SARS-CoV-2 infection. This trait was highlighted as a critical target for developing early anti-viral/anti-SARS-CoV-2 strategies. To obtain this result, analyses had been performed on transcriptome data from diverse experimental cell systems. A call was released for wide data collection of the defined set of genes for transcriptome analyses, named ‘ReprogVirus’, which should be based on strictly standardized protocols and data entry from diverse virus types and variants into the ‘ReprogVirus Platform’. This platform is currently under development. However, so far, an in vitro cell system from primary target cells for virus attacks that could ideally serve for standardizing the data collection of early SARS-CoV-2 infection responses has not been defined. Results: Here, we demonstrate transcriptome-level profiles of the most critical ‘ReprogVirus’ gene sets for identifying ‘CoV-MAC-TED’ in cultured human nasal epithelial cells infected by two SARS-CoV-2 variants differing in disease severity. Our results (a) validate ‘Cov-MAC-TED’ as a crucial trait for early SARS-CoV-2 reprogramming for the tested virus variants and (b) demonstrate its relevance in cultured human nasal epithelial cells. Conclusion: In vitro-cultured human nasal epithelial cells proved to be appropriate for standardized transcriptome data collection in the ‘ReprogVirus Platform’. Thus, this cell system is highly promising to advance integrative data analyses with the help of artificial intelligence methodologies for designing anti-SARS-CoV-2 strategies.
The Impact of Mutations on the Pathogenic and Antigenic Activity of SARS-CoV-2 during the First Wave of the COVID-19 Pandemic: A Comprehensive Immunoinformatics Analysis
Authors: Baloch Zulqarnain , Ikram Aqsa , Hakim Mohamad S. , Awan Faryal Mehwish
Published: December 2021

An in-depth analysis of first-wave SARS-CoV-2 genome is required to identify various mutations that significantly affect viral fitness. In the present study, we performed a comprehensive in silico mutational analysis of 3C-like protease (3CLpro), RNA-dependent RNA polymerase (RdRp), and spike (S) proteins with the aim of gaining important insights into first-wave virus mutations and their functional and structural impact on SARS-CoV-2 proteins. Our integrated analysis gathered 6000 SARS-CoV-2 sequences and identified 92 mutations in S, 37 in RdRp, and 11 in 3CLpro regions. The impact of these mutations was also investigated using various in silico approaches. Among these, 32 mutations in S, 15 in RdRp, and 3 in 3CLpro proteins were found to be deleterious in nature and could alter the structural and functional behavior of the encoded proteins. The D614G mutation in spike and the P323Lmutation in RdRp are the globally dominant variants with a high frequency. Most of the identified mutations were also found in the binding moiety of the viral proteins which determine their critical involvement in host–pathogen interactions and may represent drug targets. Furthermore, potential CD4+ and CD8+ T cell epitopes were predicted, and their overlap with genetic variations was explored. This study also highlights several hot spots in which HLA and drug selective pressure overlap. The findings of the current study may allow a better understanding of COVID-19 diagnostics, vaccines, and therapeutics.
Oral Administration of Coronavirus Spike Protein Provides Protection to Newborn Pigs When Challenged with PEDV
Authors: Maj Magdalena , Fake Gina M. , Walker John H. , Saltzman Ryan , Howard John A.
Published: December 2021

To investigate whether oral administration of maize-produced S antigen can provide passive immunity to piglets against porcine epidemic diarrhea virus (PEDV), 16 pregnant sows were randomly assigned to one of four treatments: (1) injection of PEDV vaccine (INJ), (2) maize grain without S protein (CON), (3) maize grain containing low dose of S antigen (LOV) and (4) maize grain containing a high dose of S antigen (HOV). Vaccines were administered on days 57, 85 and 110 of gestation. Sows’ serum and colostrum were collected at farrowing and milk on day 6 post-challenge to quantify neutralizing antibodies (NABs) and cytokines. Piglets were challenged with PEDV 3–5 d after farrowing, and severity of disease and mortality assessed on day 11 post-challenge. Disease severity was lower in LOV and INJ compared with HOV and CON, whereas the survival rate increased in piglets from LOV sows compared with HOV and CON (p ≤ 0.001). Higher titers of NABs and lower levels of cytokine granulocyte-macrophage colony-stimulating factor in sows’ milk were positively correlated with piglet survivability (p ≤ 0.05). These data suggest that feeding S protein in corn to pregnant sows protects nursing piglets against PEDV.
Analysis and Sequence Alignment of Peste des Petits Ruminants Virus ChinaSX2020
Authors: Li Lingxia , Wu Jinyan , Cao Xiaoan , He Jijun , Liu Xiangtao , Shang Youjun
Published: December 2021

The peste des petits ruminants virus (PPRV) mainly infects goats and sheep and causes a highly contagious disease, PPR. Recently, a PPRV strain named ChinaSX2020 was isolated and confirmed following an indirect immunofluorescence assay and PCR using PPRV-specific antibody and primers, respectively. A sequencing of the ChinaSX2020 strain showed a genome length of 15,954 nucleotides. A phylogenetic tree analysis showed that the ChinaSX2020 genome was classified into lineage IV of the PRRV genotypes. The genome of the ChinaSX2020 strain was found to be closely related to PPRVs isolated in China between 2013 and 2014. These findings revealed that not a variety of PRRVs but similar PPRVs were continuously spreading and causing sporadic outbreaks in China.
A Comparative View on Molecular Alterations and Potential Therapeutic Strategies for Canine Oral Melanoma
Authors: Hardwick Laura
Published: December 2021

Canine oral melanoma (COM) is a highly aggressive tumour associated with poor prognosis due to metastasis and resistance to conventional anti-cancer therapies. As with human mucosal melanoma, the mutational landscape is predominated by copy number aberrations and chromosomal structural variants, but differences in study cohorts and/or tumour heterogeneity can lead to discordant results regarding the nature of specific genes affected. This review discusses somatic molecular alterations in COM that result from single nucleotide variations, copy number changes, chromosomal rearrangements, and/or dysregulation of small non-coding RNAs. A cross-species comparison highlights notable recurrent aberrations, and functionally grouping dysregulated proteins reveals unifying biological pathways that may be critical for oncogenesis and metastasis. Finally, potential therapeutic strategies are considered to target these pathways in canine patients, and the benefits of collaboration between science, medical, and veterinary communities are emphasised.
Assessment of Changes in the Oral Microbiome That Occur in Dogs with Periodontal Disease
Authors: Santibáñez Rodrigo , Rodríguez-Salas Camila , Flores-Yáñez Carla , Garrido Daniel , Thomson Pamela
Published: December 2021

The oral microbiome in dogs is a complex community. Under some circumstances, it contributes to periodontal disease, a prevalent inflammatory disease characterized by a complex interaction between oral microbes and the immune system. Porphyromonas and Tannerella spp. are usually dominant in this disease. How the oral microbiome community is altered in periodontal disease, especially sub-dominant microbial populations is unclear. Moreover, how microbiome functions are altered in this disease has not been studied. In this study, we compared the composition and the predicted functions of the microbiome of the cavity of healthy dogs to those with from periodontal disease. The microbiome of both groups clustered separately, indicating important differences. Periodontal disease resulted in a significant increase in Bacteroidetes and reductions in Actinobacteria and Proteobacteria. Porphyromonas abundance increased 2.7 times in periodontal disease, accompanied by increases in Bacteroides and Fusobacterium. It was predicted that aerobic respiratory processes are decreased in periodontal disease. Enrichment in fermentative processes and anaerobic glycolysis were suggestive of an anaerobic environment, also characterized by higher lipopolysaccharide biosynthesis. This study contributes to a better understanding of how periodontal disease modifies the oral microbiome and makes a prediction of the metabolic pathways that contribute to the inflammatory process observed in periodontal disease.
Assessment of the Contribution of Rainwater Collection to Crop Production on Udo Island, Korea
Authors: Kang Minseok , Yoo Chulsang , Na Wooyoung
Published: December 2021

This study evaluated the contribution of small agricultural reservoirs (the mool-tongs) to crop production on Udo, and the role they play. Agricultural water demand and deficit, water storage in the mool-tongs, and the amount of irrigation water were analyzed by applying the water balance model. In particular, the Blaney-Criddle method was used to estimate the agricultural water for crop growth. In this study, four typical crops of garlic, spring onion, peanut, and barley were considered, whose growing seasons were somewhat different from each other. A total of 47 years (1973–2019) of rainfall, temperature, and evaporation data were used in this study. As a result, it was confirmed that the crop coefficient, growing period of a crop, share of the cultivated area, and the seasonal distribution of rainfall affect the role of the mool-tongs. Comparison of the frequencies of occurrence of agricultural water deficit and irrigation interval before and after introducing the mool-tongs also confirms their usefulness. After the introduction of the mool-tongs for irrigation, the frequency of occurrence of agricultural water deficits changed significantly, from almost 30% of the entire simulation period to less than 20%. Even though the water supply capacity of the mool-tongs on Udo is insufficient to offset the entire agricultural water deficit, the water stored in the mool-tongs was found to considerably aid the survival of the crops and increase crop production. The results in this study can help to determine the capacity of rainwater collecting systems, especially on a small island where the available water resources are limited.
Urban Wetlands: A Review on Ecological and Cultural Values
Authors: Alikhani Somayeh , Nummi Petri , Ojala Anne
Published: December 2021

Wetlands are a critical part of natural environments that offer a wide range of ecosystem services. In urban areas, wetlands contribute to the livability of cities through improving the water quality, carbon sequestration, providing habitats for wildlife species, reducing the effects of urban heat islands, and creating recreation opportunities. However, maintaining wetlands in urban areas faces many challenges, such as the reduction of hydrological functions, changed water regimes due to barriers, contamination by wastewater, habitat loss due to land-use change, and loss of biodiversity due to the entry of alien species. In this article, we review the theoretical background of wetlands in urban areas through the existing studies in the literature. We provide knowledge on urban wetlands and highlight the benefits of these wetlands in urban areas. These benefits include sustainability, biodiversity, urban heat islands, social perception, and recreation values. We also summarize the objectives, methodologies, and findings of the reviewed articles in five tables. In addition, we summarize the critical research gaps addressed in the reviewed articles. Our review study addresses the research gaps by performing a rigorous analysis to identify significant open research challenges, showing the path toward future research in the field. We further discuss and highlight the role of policymakers and stakeholders in preserving wetlands and finally present our conclusions.
Functional Feeding Groups of Macrofauna and Detritus Decomposition along a Gradient of Glacial Meltwater Influence in Tropical High-Andean Streams
Authors: Andino Patricio , Espinosa Rodrigo , Crespo-Pérez Verónica , Cauvy-Frauníe Sophie , Dangles Olivier , Jacobsen Dean
Published: December 2021

Tropical Andean glaciers are retreating rapidly, with possible consequences for trophic structure and ecosystem processes in high Andean meltwater streams. Here, we measured the environmental characteristics, quantified pools of particulate organic matter (POM) and periphyton (Chl. a), sampled benthic macroinvertebrates, determined functional feeding groups (FFG), and performed mesh bag decomposition experiments with Calamagrostis grass detritus at 17 stream sites along a gradient of glacial influence (GI) with 0–23% glacier cover in the catchment at 4050–4200 m a.s.l. in the Andes of Ecuador. POM was unrelated to GI while Chl. a. showed a weak (non-significant) negative relationship to GI. The macrofauna abundance decreased while taxon richness and the number of FFGs per site showed a hump-shaped relationship with increasing GI. Taxa with an opportunistic and generalist feeding mode generally dominated benthic assemblages and were related to high GI levels and low Chl. a. Only shredders were negatively related to GI, but unrelated to POM. Decomposition rates were comparable to those found in temperate alpine streams, and for both fine (0.0010–0.0065; median 0.0028 d−1) and coarse (0.0019–0.0088; median 0.0048 d−1) mesh bags, peaked at intermediate GI values, while the difference between bag types was small and almost constant along the GI gradient. This indicates an overall minor effect of macroinvertebrate shredders compared to that of microbes, in particular at high GI. It also suggests that the relatively high average temperature of these high-altitude equatorial streams (7–10 °C) does not produce higher decomposition rates than those in comparable but colder streams at temperate latitudes. The results suggest that, at the lower end of glacier cover, tropical glacier loss will not change the dominant microbial role in detritus decomposition, but that part of the physical abrasion could be partially replaced by biological shredding.
Spatiotemporal Variation on Water Quality and Trophic State of a Tropical Urban Reservoir: A Case Study of the Lake Paranoá-DF, Brazil
Authors: da Silva Damiana B. , Bellotto Valéria R. , Barbosa Jackeline do S. B. , Lima Thiago B.
Published: December 2021

Nutrient enrichment and eutrophication are among the main problems that lead to the deterioration of water quality in lakes and reservoirs. In this study, spatial and temporal variations in the concentrations of organic and inorganic species of nitrogen and phosphorus in the water column of Lake Paranoá-DF (Brazil) were evaluated between 2016 and 2017. Seasonality was the main factor in the variations in concentrations of the investigated parameters. Additionally, we found differences in behavior for different nutrients and other variables that indicate different main sources of each nutrient as well as different biogeochemical processes predominating in each season. For example, the electrical conductivity (EC), dissolved silicon, PO43−, and NO3− showed mean concentrations significantly higher in the rainy season, indicating greater inputs in these periods (which is in part related to increasing soil leaching and runoff). Agricultural activities were the main source of NO3− and wastewater treatment plants (WWTP) proved to be the main source of nutrients, mainly NH4+ and all forms of phosphorus. These two allochthonous sources are also the determining factors of the trophic state and the degradation of the water quality of Lake Paranoá. The lake is in the transition process from a mesotrophic to a eutrophic condition.
Vertical Distribution of Phytoplankton Community and Pigment Production in the Yellow Sea and the East China Sea during the Late Summer Season
Authors: Kang Jae-Joong , Min Jun-Oh , Kim Yejin , Lee Chang-Hwa , Yoo Hyeju , Jang Hyo-Keun , Kim Myung-Joon , Oh Hyun-Ju , Lee Sang-Heon
Published: December 2021

Phytoplankton community structure, which plays an important role in determining productivity and food web structure, can provide important information for understanding variations in marine ecosystems under projected climate change scenarios. Rising temperatures due to climate change will increase and intensify water stratification. To understand the community composition and distribution characteristics of phytoplankton under stratified conditions, phytoplankton pigments were analyzed in the Yellow Sea (YS) and East China Sea (ECS) during the late summer season. In addition, pigment production was measured to estimate the physiological characteristics of phytoplankton relating to light, which is an essential element of photosynthesis. During our observation period, no distinct differences were found in the community composition and pigment production of phytoplankton in the YS and the ECS, but differences in the vertical distribution were observed. Overall, the dominant phytoplankton classes at the surface depth were pico-sized cyanobacteria (46.1%), whereas micro- and nano-sized diatoms (42.9%) were the abundant most classes at a 1% light depth. The major factors controlling the vertical distributions of the phytoplankton community were temperature and nutrients (i.e., nitrate and ammonium). Cyanobacteria were positively correlated with water temperature and ammonium, whereas diatoms were negatively related to water temperature and positively correlated with nitrates. Based on the pigment production, it was found that cyanobacteria at the surface layer encountered excessive irradiance conditions during the study period. The productivity of the cyanobacterial community could be decreased under high-light and high-temperature conditions. This means that cyanobacteria could have a negative influence on the quantity and quality of food available to upper trophic organisms under warmer conditions.
Potential Ecological Risk Assessment of Heavy Metals in Cultivated Land Based on Soil Geochemical Zoning: Yishui County, North China Case Study
Authors: Yu Linsong , Zhang Fugui , Zang Kai , He Ling , Wan Fang , Liu Hongbo , Zhang Xiuwen , Shi Zeming
Published: December 2021

Various human production activities have caused tremendous damage to the soil ecological environment of cultivated land. Regional ecological risk assessments and the safe use of cultivated land have received widespread attention. The ecological risk assessment of heavy metals based on soil geochemical zoning has not been reported in the past. Using 14,389 topsoil samples, considering comprehensive geological background information, Yishui County in northern China was divided into three soil geochemical areas and 14 soil geochemical sub-regions by means of principal component factor superposition. The results of environmental quality and risk assessments of eight heavy metals based on soil geochemical zoning show that the single pollution index was greater than 1.0 and the Nemerow pollution index was greater than 0.7 for Ni in a sub-region, indicating that Ni pollution had reached the early warning limit, which demonstrates that Ni has a certain enrichment trend. Meanwhile, the geoaccumulation index of Ni and Cr was greater than zero in some sub-regions, indicating a slight pollution level. In addition, the potential ecological risk factor of the measured heavy metals was greater than 40 in 9 sub-regions, indicating a moderate ecological hazard, and the risk index was greater than 150 in a sub-region, revealing moderate ecological intensity, in which Hg and Cd were leading contributors to potential ecological hazards with a contribution rate between 58% and 76%. This method is suitable for the evaluation of soil environmental quality and safety for medium and large scales, and can provide a scientific basis for further zoning and grading prevention and control of soil pollution in cultivated land.
Visual Harmony of Engineering Structures in a Mountain Stream
Authors: Chen Jinn-Chyi , Huang Chia-Ling , Chen Su-Chin , Tfwala Samkele S.
Published: December 2021

This study uses the cognitive factor of “visual harmony” to assess the visual quality of stream engineering in a mountainous region. Images of engineering structures such as revetments and submerged dams in the mountain streams of Taiwan were collected. Three image groups with different structures invaded by vegetation were used for a questionnaire survey, which yielded 154 valid samples. We used statistical analysis to develop a model of visual harmony H with respect to the percentage of visible greenery GR, that is, the perceived curve of vegetation change. A comparison of our data with the literature determined the upper and lower bound curves of the relationship between H and GR. We found that the physical elements of “softscape” and “hardscape”—namely, percentage of visible water WR, visible structure IR, and visible natural material on the structure NR—affected this relationship. Results show that H is equivalent to visual preference P, and both can be improved by better green visibility (increasing GR and GR < 50%), avoiding low water visibility (WR < 10%), or increasing the amount of visible natural material (NR > 0.9). High visibility of the structures (IR > 0.3) may decrease H and P. We ultimately propose a visual harmony or preference model concerning a combined physical indicator that comprises GR, WR, IR and NR. Results of this study could be helpful to improve or access the aesthetics of stream engineering design.
Efficient Adsorption of Lead Ions from Synthetic Wastewater Using Agrowaste-Based Mixed Biomass (Potato Peels and Banana Peels)
Authors: Ashfaq Aamna , Nadeem Razyia , Bibi Shamsa , Rashid Umer , Hanif Muhammad Asif , Jahan Nazish , Ashfaq Zeeshan , Ahmed Zubair , Adil Muhammad , Naz Maleeha
Published: December 2021

The aquatic environment is continuously being polluted by heavy metals released from industrial, domestic, agricultural, and medical applications. It is difficult to remove heavy metals, as they are nonbiodegradable. Heavy metals cause genotoxicity and serious carcinogenic disorders. Various conventional methods have been used for the removal of heavy metals, but these are time-consuming and not economical, so green methods, being economical, are preferred over conventional methods. Adsorption, being effective, environmentally friendly, and cheap, is often preferred. The present investigation investigated the adsorption efficiency of agrowaste-based biosorbents for removal of Pb(II) ion from the synthetic wastewater. Mixed biomass of banana peel and potato peels was used to create biosorbents. The biosorbents were characterized in terms of structural and surface morphology by SEM, while functional groups were analyzed by FT-IR and XRD analysis. The adsorption of Pb(II) was studied by a batch method, and various experimental parameters were studied. Optimum conditions for the removal of lead were pH = 5, concentration = 10 ppm, adsorbent dosage = 1.0 g, and contact time = 2 h. Kinetic modelling studies showed that the adsorption of Pb(II) ions followed a pseudo-second-order mechanism, and the Langmuir isotherm model was found to fit well for this study. Highlights: Synthesis of biosorbents (mixed biomass of potato peel and banana peel, biochar, TiO2 nanocomposites). Characterization of prepared biosorbents (SEM, XRD, FT-IR). Optimized parameters (pH, initial concentration, adsorbent dosage, and contact time) for removal of pollutant.
The Effect of Temporal Characteristics on Developing a Practical Rainfall-Induced Landslide Potential Evaluation Model Using Random Forest Method
Authors: Huang Yi-Min , Lu Shao-Wei
Published: December 2021

With the unique rainfall patterns of typhoons, plum rains, and short-term heavy rainfalls, the frequent landslide and debris flow disasters have caused severe loss to people in Taiwan. In the studies of landslide susceptibility, the information of factors used for analysis was usually annual-based content, and it was assumed that the same elements from different years were independent between each year. However, the occurrence of landslides was usually not simply due to the changes within a year. Instead, landslides were triggered because the factors that affected the potential of landslides reached critical conditions after a cumulative change with time. Therefore, this study had well evaluated the influence of temporal characteristics and the ratios of antecedent landslide areas in the past five years in the landslide potential evaluation model. The analysis was conducted through the random forest (RF) algorithm. Additional rainfall events of 2017 were used to test the proposed model’s performance to understand its practicality. The analysis results show that in the study area, the RF model had considerably acceptable performance. The results have also demonstrated that the antecedent landslide ratios in the past five years were essential to describe the significance of cumulative change with time when conducting potential landslide evaluation.
Citizen Science for Marine Litter Detection and Classification on Unmanned Aerial Vehicle Images
Authors: Merlino Silvia , Paterni Marco , Locritani Marina , Andriolo Umberto , Gonçalves Gil , Massetti Luciano
Published: December 2021

Unmanned aerial vehicles (UAV, aka drones) are being used for mapping macro-litter in the environment. As drone images require a manual processing task for detecting marine litter, it is of interest to evaluate the accuracy of non-expert citizen science operators (CSO) in performing this task. Students from Italian secondary schools (in this work, the CSO) were invited to identify, mark, and classify stranded litter items on a UAV orthophoto collected on an Italian beach. A specific training program and working tools were developed for the aim. The comparison with the standard in situ visual census survey returned a general underestimation (50%) of items. However, marine litter bulk categorisation was fairly in agreement with the in situ survey, especially for sources classification. The concordance level among CSO ranged between 60% and 91%, depending on the item properties considered (type, material, and colour). As the assessment accuracy was in line with previous works developed by experts, remote detection of marine litter on UAV images can be improved through citizen science programs, upon an appropriate training plan and provision of specific tools.
Trust, Risk, and Power in Transboundary Aquifer Assessment Collaborations
Authors: Brause Holly
Published: December 2021

In events and discussions about transboundary aquifer assessment, trust is often cited as an essential component of collaborative efforts. However, there is little discussion of what trust is, how it is built, what diminishes trust, and why it is so important. This study uses ethnographic research carried out between 2019 and 2021 with the Transboundary Aquifer Assessment Program (TAAP) to examine the role and significance of trust in U.S./Mexico TAAP collaborations. This study demonstrates that trust is best understood in relationship to power and risk. It examines the strengths and weaknesses of the TAAP program in managing asymmetrical relationships of power and unequal levels of risk in participation. In TAAP collaborations, the insistence on establishing trust should signal participants to consider and address the underlying issues of risk and power.