bluuebunny/crossref_metadata_2025 huggingface dataset:
Energy-Saving SSD Cache Management for Video Servers with Heterogeneous HDDs
Authors: Kim Kyungmin , Song Minseok
Published: May 2022

Dynamic adaptive streaming over HTTP (DASH) technique, the most popular streaming method, requires a large number of hard disk drives (HDDs) to store multiple bitrate versions of many videos, consuming significant energy. A solid-state drive (SSD) can be used to cache popular videos, thus reducing HDD energy consumption by allowing I/O requests to be handled by an SSD, but this requires effective HDD power management due to limited SSD bandwidth. We propose a new SSD cache management scheme to minimize the energy consumption of a video storage system with heterogeneous HDDs. We first present a technique that caches files with the aim of saving more HDD energy as a result of I/O processing on an SSD. Based on this, we propose a new HDD power management algorithm with the goal of increasing the number of HDDs operated in low-power mode while reflecting the heterogeneous HDD power characteristics. For this purpose, it assigns a separate parameter value to each I/O task based on the ratio of HDD energy to bandwidth and greedily selects the I/O tasks handled by the SSD within limits on its bandwidth. Simulation results show that our scheme consumes between 12% and 25% less power than alternative schemes under the same HDD configuration.
Real Time Hardware-in-Loop Implementation of LLC Resonant Converter at Worst Operating Point Based on Time Domain Analysis
Authors: Geddam Kiran Kumar , Devaraj Elangovan
Published: May 2022

The inductor inductor capacitor (LLC) resonant topology has become more popular for deployment in high power density and high-efficiency power converter applications due to its ability to maintain zero voltage switching (ZVS) over a wider input voltage range. Due to their ease of operation and acceptable accuracy, frequency domain-related analytical methods using fundamental harmonic approximation (FHA) have been frequently utilized for resonant converters. However, when the switching frequency is far from the resonant frequency, the circuit currents contain a large number of harmonics, which cannot be ignored. Therefore, the FHA is incapable of guiding the design when the LLC converter is used to operate in a wide input voltage range applications due to its inaccuracy. As a result, a precise LLC converter model is needed. Time domain analysis is a precise analytical approach for obtaining converter attributes, which supports in the optimal sizing of LLC converters. This work strives to give a precise and an approximation-free time domain analysis for the exact modeling of high-frequency resonant converters. A complete mathematical analysis for an LLC resonant converter operating in discontinuous conduction mode (DCM)—i.e., the boost mode of operation below resonance—is presented in this paper. The proposed technique can confirm that the converter operates in PO mode throughout its working range; in addition, for primary MOSFET switches, it guarantees the ZVS and zero current switching (ZCS) for the secondary rectifier. As a function of frequency, load, and other circuit parameters, closed-form solutions are developed for the converter’s tank root mean square (RMS) current, peak stress, tank capacitor voltage, voltage gain, and zero voltage switching angle. Finally, an 8 KW LLC resonant converter is built in the hardware-in-loop (HIL) testing method on RT-LAB OP-5700 to endorse the theoretical study.
Source-Load Coordinated Low-Carbon Economic Dispatch of Electric-Gas Integrated Energy System Based on Carbon Emission Flow Theory
Authors: Feng Jieran , Nan Junpei , Wang Chao , Sun Ke , Deng Xu , Zhou Hao
Published: May 2022

The development of emerging technologies has enhanced the demand response (DR) capability of conventional loads. To study the effect of DR on the reduction in carbon emissions in an integrated energy system (IES), a two-stage low-carbon economic dispatch model based on the carbon emission flow (CEF) theory was proposed in this study. In the first stage, the energy supply cost was taken as the objective function for economic dispatch, and the actual carbon emissions of each energy hub (EH) were calculated based on the CEF theory. In the second stage, a low-carbon DR optimization was performed with the objective function of the load-side carbon trading cost. Then, based on the modified IEEE 39-bus power system/Belgian 20-node natural gas system, MATLAB/Gurobi was used for the simulation analysis in three scenarios. The results showed that the proposed model could effectively promote the system to reduce the load peak-to-valley difference, enhance the ability to consume wind power, and reduce the carbon emissions and carbon trading cost. Furthermore, as the wind power penetration rate increased from 20% to 80%, the carbon reduction effect basically remained stable. Therefore, with the growth of renewable energy, the proposed model can still effectively reduce carbon emissions.
A Bi-Objective Sustainable Supply Chain Redesign: What Effect Does Energy Availability Have on Redesign?
Authors: Hanczar Paweł , Azadehranjbar Zahra
Published: May 2022

During supply chain redesign processes, many environmental initiatives at facilities and their surrounding regions are affected by the closing, opening, or even broadening of facilities. These initiatives may be about the surplus renewable energies that the facility generates for its region, the renewable energy sources that are available in the area for the facility, or the ecological relationships between facilities and their surrounding regions in general. The implications of these ecological relationships for the supply chain redesign problem were considered in this study in order to minimize the negative outcomes while minimizing the process’s related expenses. Thus, to minimize the unwanted effects, an objective function is defined in addition to the cost objective, which results in a bi-objective problem. Although small cases can be solved by exact methods such as AUGMECON, for larger scales, a matheuristic algorithm needs to be developed. The efficiency of the developed algorithm was investigated through numerical instances as well as with different metrics including a Pareto analysis. The results at larger scales show a shorter runtime of the matheuristic compared to the AUGMECON algorithm. Additionally, the matheuristic method provides a wider range of decision areas for managers.
Improving the Maximum Power Extraction from Wind Turbines Using a Second-Generation CRONE Controller
Authors: Yessef Mourad , Bossoufi Badre , Taoussi Mohammed , Motahhir Saad , Lagrioui Ahmed , Chojaa Hamid , Lee Sanghun , Kang Byeong-Gwon , Abouhawwash Mohamed
Published: May 2022

Developing precise and robust algorithms that can help in obtaining maximum power yield in a variable speed wind turbine is an important area of research in wind engineering. The present manuscript proposes a technique that utilizes a second-generation CRONE controller for the maximum power tracking technique (MPPT) to maximize power generation in a wind energy conversion system (WECS) based on a double-fed induction generator (DFIG). The authors propose this novel method because the classical controllers cannot provide adequate performance in terms of extracting the maximum energy from variable speed wind turbines when applying a real wind profile and they cannot guarantee the high stability of the WECS. Moreover, this novel controller sufficiently handles problems related to the control effort level. The performance of the second-generation CRONE method was mathematically modeled using MATLAB/Simulink and compared with four other types of MPPT control techniques, which include a proportional-integral linear controller (PI), nonlinear sliding mode controller (SMC), backstepping controller (BS), and fuzzy logic controller (FLC). Two different wind profiles, a step wind profile and a real wind profile, were considered for the comparative study. The response time, dynamic error percentage, and static error percentage were the quantitative parameters compared, and the qualitative parameters included set-point tracking and precision. This test demonstrated the superiority of the second-generation CRONE controller in terms of all of the compared parameters.
Endogenous Hormones and Biochemical Changes during Flower Development and Florescence in the Buds and Leaves of Lycium ruthenicum Murr
Authors: Guo Youyan , An Lizhe , Yu Hongyuan , Yang Miaomiao
Published: May 2022

Lycium ruthenicum Murr. is one of the most important shrubs grown in northwest China. Healthy buds and leaves of L. ruthenicum Murr. were selected for the present study. Flower development was divided into six stages, namely, flower bud pre-differentiation (I), late flower differentiation (II), squaring stage (III), dew crown period (IV), open stage (V) and senescent stage (VI). Endogenous hormone content and specific value, soluble sugar, sucrose, starch, and soluble protein were measured, and ABA/IAA, ABA/GA3, ZR/IAA, ZR/GA3, and C/N were calculated in buds and leaves at stage VI. The results showed that ABA, GA3, and ZR content of buds significantly increased from flower bud pre-differentiation to late flower differentiation stage. However, ABA, GA3, and ZR content of leaves had the opposite change trend. From open stage to senescent stage, IAA, ABA, and GA3 content of buds and leaves significantly increased in L. ruthenicum Murr. However, ZR content of buds and leaves significantly decreased from open stage to senescent stage. ABA/IAA, ABA/GA3, ZR/IAA, and ZR/GA3 values of buds significantly increased from lower bud pre-differentiation to late flower differentiation stage. However, ABA/IAA, ABA/GA3, ZR/IAA, and ZR/GA3 values of leaves significantly decreased from lower bud pre-differentiation to late flower differentiation stage. ABA/IAA and ABA/GA3 of buds significantly increased from open stage to senescent stage, but ZR/IAA and ZR/GA3 of buds significantly decreased from open to senescent. At this stage, ABA/IAA, ABA/GA3, ZR/IAA, and ZR/GA3 significantly decreased in L. ruthenicum Murr. The higher soluble sugar and sucrose content in the buds and leaves were beneficial to the flower bud differentiation of L. ruthenicum. The increasing of soluble sugar improved the energy basis to florescence and senescent. The carbohydrates metabolism enhanced from open stage to senescent stage and nitrogen metabolism reduced from open stage to senescent stage of L. ruthenicum.
Effects of γ-Irradiation on Structure and Functional Properties of Pea Fiber
Authors: Cheng Tianfu , Liu Caihua , Hu Zhaodong , Wang Zhongjiang , Guo Zengwang
Published: May 2022

In this study, pea residue reserve insoluble diet fiber (hereinafter referred to as pea fiber) was used as a raw material. The effects of γ-irradiation doses (0, 0.5, 1, 2, 3, and 5 kGy) on the structural properties (main composition, particle size and specific surface area, scanning electron microscope (SEM) microstructure, Fourier transform infrared spectroscopy, and X-ray diffraction) and functional properties (oil-holding capacity, swelling and water-holding capacity, and adsorption properties) of pea fiber were explored. The results show that, when the γ-irradiation dose was 2 kGy, compared with the untreated sample, the contents of cellulose, hemicellulose and lignin in pea fiber decreased by 1.34 ± 0.42%, 2.56 ± 0.03% and 2.02 ± 0.05%, respectively, and the volume particle size of pea fiber decreased by 17.43 ± 2.35 μm. The specific surface area increased by 23.70 ± 2.24 m2/kg and the crystallinity decreased by 7.65%. Pore and irregular particles appeared on the microstructure surface of the pea fiber treated with γ-irradiation. The results of the infrared spectrum showed that the hemicellulose and lignin in pea fiber were destroyed by γ-irradiation. These results indicate that γ-irradiation can significantly affect the structural properties of pea fiber. When the γ-irradiation dose was 2 kGy, the highest oil-holding capacity, swelling capacity and water-holding capacity of pea fiber were 8.12 ± 0.12 g/g, 19.75 ± 0.37 mL/g and 8.35 ± 0.18 g/g, respectively, and the adsorption capacities of sodium nitre, cholesterol and glucose were also the strongest. These results indicate that the functional properties of pea fiber are improved by γ-irradiation. In this study, γ-irradiation technology was used as pretreatment to provide a theoretical basis for the application of pea fiber in food processing.
On a Partial Fractional Hybrid Version of Generalized Sturm–Liouville–Langevin Equation
Authors: Heydarpour Zohreh , Izadi Javad , George Reny , Ghaderi Mehran , Rezapour Shahram
Published: May 2022

As we know one of the most important equations which have many applications in various areas of physics, mathematics, and financial markets, is the Sturm–Liouville equation. In this paper, by using the α-ψ-contraction technique in fixed point theory and employing some functional inequalities, we study the existence of solutions of the partial fractional hybrid case of generalized Sturm–Liouville-Langevin equations under partial boundary value conditions. Towards the end, we present two examples with numerical and graphical simulation to illustrate our main results.
Robust Silica–Agarose Composite Aerogels with Interpenetrating Network Structure by In Situ Sol–Gel Process
Authors: Yang Xin , Jiang Pengjie , Xiao Rui , Fu Rui , Liu Yinghui , Ji Chao , Song Qiqi , Miao Changqing , Yu Hanqing , Gu Jie , Wang Yaxiong , Sai Huazheng
Published: May 2022

Aerogels are three-dimensional nanoporous materials with outstanding properties, especially great thermal insulation. Nevertheless, their extremely high brittleness restricts their practical application. Recently, although the mechanical properties of silica aerogels have been improved by regulating the precursor or introducing a polymer reinforcer, these preparation processes are usually tedious and time-consuming. The purpose of this study was to simplify the preparation process of these composite aerogels. A silicic acid solution treated with cation exchange resin was mixed with agarose (AG) to gel in situ, and then composite aerogels (CAs) with an interpenetrating network (IPN) structure were obtained by aging and supercritical CO2 fluid (SCF) drying. Compared to previous works, the presented CAs preparation process is briefer and more environmentally friendly. Moreover, the CAs exhibit a high specific surface area (420.5 m2/g), low thermal conductivity (28.9 mW m−1 K−1), excellent thermal insulation properties, and thermal stability. These results show that these CAs can be better used in thermal insulation.
Hydrophobization of Monolithic Resorcinol-Formaldehyde Xerogels by Means of Silylation
Authors: Henn Fabian , Tannert René
Published: May 2022

In materials research, the control of wettability is important for many applications. Since they are typically based on phenolics, organic aerogels, and xerogels are intrinsically hydrophilic in nature, and examples of the chemical functionalization of such gels are scarce and often limited to powders. This study reports on the silylation of monolithic resorcinol-formaldehyde (RF) xerogels using solutions of silyl chlorides and triflates, respectively, in combination with an amine base. The resulting gels are structurally characterized by means of elemental analysis, X-ray photoelectron spectroscopy, pycnometry, sorption analysis, and scanning electron microscopy with electron-dispersive X-ray spectroscopy. The wetting behavior of the silylated gels was studied by the determination of the contact angle to water after exposure of the gels to ambient air. Additionally, the uptake of liquid water and aqueous acids and bases was investigated. As a result, processes for the functionalization of RF xerogels with sterically demanding silyl moieties have been established. Although the analyses indicate that silylation occurred to a rather small extent, highly hydrophobic gels resulted which retained the wetting behavior over the course of several months with contact angles of >130°. Monoliths bearing sterically demanding silyl groups showed higher stability towards aqueous acid than trimethylsilylated RF gels.
Recent Research on Hybrid Hydrogels for Infection Treatment and Bone Repair
Authors: Cao Mengjiao , Liu Chengcheng , Li Mengxin , Zhang Xu , Peng Li , Liu Lijia , Liao Jinfeng , Yang Jing
Published: May 2022

The repair of infected bone defects (IBDs) is still a great challenge in clinic. A successful treatment for IBDs should simultaneously resolve both infection control and bone defect repair. Hydrogels are water-swollen hydrophilic materials that maintain a distinct three-dimensional structure, helping load various antibacterial drugs and biomolecules. Hybrid hydrogels may potentially possess antibacterial ability and osteogenic activity. This review summarizes the recent progress of different kinds of antibacterial agents (including inorganic, organic, and natural) encapsulated in hydrogels. Several representative hydrogels of each category and their antibacterial mechanism and effect on bone repair are presented. Moreover, the advantages and disadvantages of antibacterial agent hybrid hydrogels are discussed. The challenge and future research directions are further prospected.
Last Aid Training Online: Participants’ and Facilitators’ Perceptions from a Mixed-Methods Study in Rural Scotland
Authors: Macaden Leah , Broadfoot Kirsten , Carolan Clare , Muirhead Kevin , Neylon Siobhan , Keen Jeremy
Published: May 2022

(1) Background: Palliative and end-of-life care services are increasingly gaining centre stage in health and social care contexts in the UK and globally. Death and dying need are relational processes. Building personal and community capacity along with resilience is vital to support families and communities to normalise death and dying. Last Aid Training (LAT) is one such innovative educational initiative which teaches the general public about the fundamentals of palliative care and promotes public discussion about death and dying. The Highland Hospice [HH] in Scotland has pioneered delivery of LAT in face-to-face settings since March 2019 and online since March 2020 to accommodate pandemic restrictions. (2) Methods: This study used a mixed-methods approach, combining an online survey with LAT participants followed by individual semi-structured qualitative interviews with both LAT participants and facilitators. The primary aim of this study was to investigate the impacts of LAT for participants at the individual, family, and community levels, as well as explore participant and facilitator experiences and perspectives of LAT in an online environment. (3) Results: Overall, this evaluation demonstrates that provision of foundational death literacy education in social contexts enhances the personal knowledge, skills, and confidence of individual community members and supports the notion that this personal growth could lead to strengthened community action. (4) Conclusions: Findings from this study concluded that there is potential to include LAT as the foundational core training to promote death literacy in communities with further exploration to integrate/align LAT with other national/global end-of-life care frameworks.
Larvicidal Activity and Phytochemical Profiling of Sweet Basil (Ocimum basilicum L.) Leaf Extract against Asian Tiger Mosquito (Aedes albopictus)
Authors: Chan Chee Aun , Ho Lai Yee , Sit Nam Weng
Published: May 2022

Applying larvicides to interrupt a mosquito’s life cycle is an important strategy for vector control. This study was conducted to evaluate the larvicidal properties of the hexane extract of sweet basil (Ocimum basilicum L.; family Lamiaceae) leaves against the wild strain of Asian tiger mosquito, Aedes albopictus (Skuse). Third instar larvae (20 larvae/replicate, n = 3) were exposed to different concentrations of the extract (6.25–200 µg/mL), and the mortality rate was recorded. Probit analysis showed that the median lethal concentration and 95% lethal concentration of the extract were 16.0 (10.9–22.1) and 53.0 (34.6–136.8) µg/mL, respectively, after 24 h exposure. Only the fractions F3, F4, and F5 from the column chromatography displayed high mortality rates of 91.7–100% at 25.0 µg/mL after 24 h exposure. Subsequent column chromatography from the pooled fraction yielded two active subfractions, H-F345-S2 and H-F345-S3, with mortality rates of 100% and 98.3 ± 2.9%, respectively, at 12.5 µg/mL. Gas chromatography–mass spectrometry analysis unveiled that methyl chavicol, 2-(2-butoxyethoxy)ethanol, cedrelanol, methyl eugenol, 2,4,di-tert-butylphenol, and phytol were the major components in both subfractions with some of them being reported as larvicidal compounds. The results suggest that sweet basil has substantial larvicidal activity against Ae. albopictus mosquito and is a potential source of naturally derived larvicide.
Effects of Vermicompost Substrates and Coconut Fibers Used against the Background of Various Biofertilizers on the Yields of Cucumis melo L. and Solanum lycopersicum L.
Authors: Mejía Pedro A. , Ruíz-Zubiate José Luis , Correa-Bustos Amelia , López-López María José , Salas-Sanjuán María del Carmen
Published: May 2022

Vermicompost has been promoted as a viable substrate component owing to its physicochemical properties, nutrient richness, and status as an excellent soil improver. It is considered the best organic fertilizer and is more eco-friendly than chemical fertilizers. Plant-growth-promoting microorganisms (PGPMs) are defined as plant biofertilizers that improve nutritional efficiency—that is, they transform nutrients within substrates from organic to inorganic forms, making them available for plants. The main objective of this research study is to evaluate the effects of the application of three PGPM microbial consortia on different mixtures of organic substrates based on vermicompost (V) and coconut fiber (CF) on two different horticultural crops. We performed a yield analysis and drainage nutrient tests and determined the plant nutritional status and enzymatic activity in organic substrates based on the two crops, Cucumis melo L. and Solanum lycopersicum L. A multivariate analysis of variance and principal component analysis was conducted using substrate types and PGPMs as factors. Differences (p < 0.05) in yield, dehydrogenase activity, the nutrient concentrations in a petiole sap, and drainage were observed at 30, 60, 75, and 90 days after transplant. PGPMs such as Trichoderma sp. and plant-growth-promoting rhizobacteria (PGPR) in organic substrates (40V + 60CF) can significantly improve the nutritional status of plants for use in organic soilless container agriculture. Biofertilization with PGPMs and suitable mixtures of organic substrates together with aqueous extracts (tea) of vermicompost, as nutrient solutions applied by fertigation, has allowed us to achieve an adequate level of production through environmentally friendly techniques. The results obtained allowed us to affirm that it was possible to replace conventional fertilization using no chemical products and ensure adequate crop nutrition by supplying main macronutrients with organic sources and biofertilizers.
Use of Information and Communication Technology by South Korean Occupational Therapists Working in Hospitals: A Cross-Sectional Study
Authors: Hwang Na-Kyoung , Shim Sun-Hwa , Cheon Hye-Won
Published: May 2022

The convergence and development of information and communication technology (ICT) have brought changes to occupational therapy practices, posing novel challenges for occupational therapists (OTs). This study aimed to investigate current practices of ICT use and factors affecting the clinical use of ICT among Korean OTs. An online survey was conducted among 158 domestic OTs working in hospitals. Participants reported that the therapeutic use of ICT positively affected client outcomes, ICT choice, and continued use. Participants highlighted the necessity to assess the ability of clients to use smart devices and ensure familiarity in the OT process. Of respondents, 31% reported the application of ICT-based interventions or recommendations in clinical practice. The use of ICT was predominantly associated with cognitive function, leisure activities, and information access and communication. A significant difference in barriers to ICT use was observed between familiar users and non-users. Familiar users reported a lack of knowledge and training as major barriers, whereas non-users reported expensive products or technology. Ease of use and usefulness were facilitators of ICT use among familiar users. Information and training opportunities are required to promote ICT use by OTs, and the usefulness of ICT must be realized via client-centered, customized approaches.
Prevalence of High-Risk β-Lactam Resistance Genes in Family Livestock Farms in Danjiangkou Reservoir Basin, Central China
Authors: Yang Fengxia , Zhang Zulin , Li Zijun , Han Bingjun , Zhang Keqiang , Yang Peng , Ding Yongzhen
Published: May 2022

The propagation of antibiotic resistance genes (ARGs) from domestic livestock manure is an unnegligible important environmental problem. There is an increasing need to understand the role of domestic livestock manure in causing antibiotic resistance in the environment to minimize risks to human health. Here, we targeted β-lactam resistance genes (bla genes), primarily discovered in clinical settings, to compare the high-risk ARG profile and their main spreading vectors of 26 family livestock farms in China and analyze the effects of domestic livestock manure on their receiving farmland environments. Results showed that the high-risk bla genes and their spreading carriers were widely prevalent in livestock and poultry manure from family farms. The blaampC gene encoding extended-spectrum AmpC β-lactamases, as well as its corresponding spreading carrier (class-1 integron), had the highest occurrence level. The bla gene abundance in family chicken farms was higher than that in family swine and cattle farms, while the bla gene contamination in the feces of laying hens or beef cattle was worse than that in corresponding broiler chickens or dairy cattle. Notably, the application from domestic livestock manure led to substantial emission of bla genes, which significantly increased the abundance of high-risk resistance genes in farmland soil by 12–46 times. This study demonstrated the prevalence and severity of high-risk resistance genes in domestic livestock and poultry manure; meanwhile, the discharge of bla genes also highlighted the need to mitigate the persistence and spread of these elevated high-risk genes in agricultural systems.
Behavior in Avalanche Terrain: An Exploratory Study of Illegal Snowmobiling in Norway
Authors: Michaelsen Bjørn , Stewart-Patterson Iain , Rolland Carsten G. , Hetland Audun , Engeset Rune V.
Published: May 2022

Snowmobilers make a grim and significant contribution to avalanche fatality statistics in Norway. However, there is limited knowledge on the behavior of this group in avalanche terrain and the factors influencing this behavior. Our study documents what snowmobilers do and not do in avalanche terrain, how their behavior relates to managing complex avalanche conditions and if there is a mismatch between avalanche competence, education and riding preferences. This ethnographic study observed snowmobiler tracks and thus avalanche terrain usage in Northern Norway during 2018 and 2019, supported by open-ended conversations with target group riders. Results show that high-marking lost popularity to technical riding, which seems to be perceived as safer despite increased exposure to complex avalanche terrain and conditions with persistent weak layers in the snowpack. The detected mismatch between preferences and avalanche knowledge/attitude will remain an obstacle to future accident prevention efforts unless behavioral changes are addressed. This study of a predominantly illegal activity sheds light on how to explore and observe hard-to-reach illegal activities and should be of interest to a wider audience from other research disciplines.
Health-Promoting Quality of Life at Work during the COVID-19 Pandemic: A 12-Month Longitudinal Study on the Work-Related Sense of Coherence in Acute Care Healthcare Professionals
Authors: Berger-Estilita Joana , Abegglen Sandra , Hornburg Nadja , Greif Robert , Fuchs Alexander
Published: May 2022

(1) Background: During a pandemic, the wellbeing of healthcare professionals is crucial. We investigated the long-term association of the Work-related Sense of Coherence (Work-SoC) and the evolution of psychological health symptoms of acute care healthcare professionals during the first year of the COVID-19 pandemic. (2) Methods: This longitudinal observational study enrolled 520 multinational healthcare professionals, who completed an online survey every three months from April 2020 to April 2021. Mixed linear models examined the associations between Work-SOC and COVID-19-related anxiety, perceived vulnerability, depressiveness, and psychological trauma symptomatology. (3) Results: Healthcare professionals with a higher Work-SoC reported lower levels of COVID-19-related anxiety, perceived vulnerability, depressiveness, and psychological trauma symptomatology in April 2020 than healthcare professionals with an average or lower Work-SoC, but the levels increased to higher values in April 2021. Healthcare professionals with a lower Work-SoC reported higher levels of depressiveness and psychological trauma symptomatology in April 2020 but lower levels in April 2021. (4) Conclusions: Healthcare professionals with higher levels of Work-related Sense of Coherence might be protected against variations in psychological symptoms for about three months, but this protection seems to decrease as the pandemic continues, resulting in mental health deterioration. In contrast, healthcare professionals with a lower Work-SoC might be protected at later stages of the pandemic.
Decreased Expression of Plakophilin-2 and αT-Catenin in Arrhythmogenic Right Ventricular Cardiomyopathy: Potential Markers for Diagnosis
Authors: Hung Pei-Fang , Chung Fa-Po , Hung Chung-Lieh , Lin Yenn-Jiang , Kuo Tzu-Ting , Liao Jo-Nan , Chen Yun-Yu , Pan Chih-Hsin , Shaw Kai-Ping , Chen Shih-Ann
Published: May 2022

Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a hereditary disease of the heart muscle. Clinical challenges remain, however, in identifying patients with ARVC in the early or concealed stages with subtle clinical manifestations. Therefore, we wanted to identify potential targets by immunohistochemical (IHC) analysis in comparison with controls. Pathogenic mutations were identified in 11 of 37 autopsied patients with ARVC. As observed from IHC analysis of the RV, expression of αT-catenin and plakophilin-2 is significantly decreased in autopsied patients with ARVC as compared to controls, and the decreased expression is consistent in patients with and without pathogenic mutations. Furthermore, ARVC specimens demonstrated a reduced localization of αT-catenin, desmocollin-2, desmoglein-2, desmoplakin, and plakophilin-2 on intercalated discs. These findings have been validated by comparing RV specimens obtained via endomyocardial biopsy between patients with ARVC and those without. The pathogenic mutation was present in 3 of 5 clinical patients with ARVC. In HL-1 myocytes, siRNA was used to knockdown CTNNA3, and western blotting analysis demonstrated that the decline in αT-catenin expression was accompanied by a significant decline in the expression of plakophilin-2. The aforementioned effect was directed towards protein degradation rather than mRNA stability. Plakophilin-2 expression decreases concurrently with the decline in CTNNA3 expression. Therefore, the expression of αT-catenin and plakophilin-2 could be potential surrogates for the diagnosis of ARVC.
Complex Mutation Pattern of Omicron BA.2: Evading Antibodies without Losing Receptor Interactions
Authors: Kannan Saathvik R. , Spratt Austin N. , Sharma Kalicharan , Goyal Ramesh , Sönnerborg Anders , Apparsundaram Subbu , Lorson Christian L. , Byrareddy Siddappa N. , Singh Kamal
Published: May 2022

BA.2, a sublineage of Omicron BA.1, is now prominent in many parts of the world. Early reports have indicated that BA.2 is more infectious than BA.1. To gain insight into BA.2 mutation profile and the resulting impact of mutations on interactions with receptor and/or monoclonal antibodies, we analyzed available sequences, structures of Spike/receptor and Spike/antibody complexes, and conducted molecular dynamics simulations. The results showed that BA.2 had 50 high-prevalent mutations, compared to 48 in BA.1. Additionally, 17 BA.1 mutations were not present in BA.2. Instead, BA.2 had 19 unique mutations and a signature Delta variant mutation (G142D). The BA.2 had 28 signature mutations in Spike, compared to 30 in BA.1. This was due to two revertant mutations, S446G and S496G, in the receptor-binding domain (RBD), making BA.2 somewhat similar to Wuhan-Hu-1 (WT), which had G446 and G496. The molecular dynamics simulations showed that the RBD consisting of G446/G496 was more stable than S446/S496 containing RBD. Thus, our analyses suggested that BA.2 evolved with novel mutations (i) to maintain receptor binding similar to WT, (ii) evade the antibody binding greater than BA.1, and (iii) acquire mutation of the Delta variant that may be associated with the high infectivity.
Down-Regulating the High Level of 17-Beta-Hydroxysteroid Dehydrogenase 13 Plays a Therapeutic Role for Non-Alcoholic Fatty Liver Disease
Authors: Wang Meixi , Li Jianrui , Li Hu , Dong Biao , Jiang Jing , Liu Nannan , Tan Jiali , Wang Xuekai , Lei Lei , Li Hongying , Sun Han , Tang Mei , Wang Huiqiang , Yan Haiyan , Li Yuhuan , Jiang Jiandong , Peng Zonggen
Published: May 2022

Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease worldwide, and there is no specific drug to treat it. Recent results showed that 17-beta-hydroxysteroid dehydrogenase type 13 (HSD17B13) is associated with liver diseases, but these conclusions are controversial. Here, we showed that HSD17B13 was more highly expressed in the livers of NAFLD patients, and high expression was induced in the livers of murine NAFLD models and cultural hepatocytes treated using various etiologies. The high HSD17B13 expression in the hepatocytes facilitated the progression of NAFLD by directly stabilizing the intracellular lipid drops and by indirectly activating hepatic stellate cells. When HSD17B13 was overexpressed in the liver, it aggravated liver steatosis and fibrosis in mice fed with a high-fat diet, while down-regulated the high expression of HSD17B13 by short hairpin RNAs produced a therapeutic effect in the NAFLD mice. We concluded that high HSD17B13 expression is a good target for the development of drugs to treat NAFLD.
MicroRNAs: Important Regulatory Molecules in Acute Lung Injury/Acute Respiratory Distress Syndrome
Authors: Lu Qianying , Yu Sifan , Meng Xiangyan , Shi Mingyu , Huang Siyu , Li Junfeng , Zhang Jianfeng , Liang Yangfan , Ji Mengjun , Zhao Yanmei , Fan Haojun
Published: May 2022

Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is an overactivated inflammatory response caused by direct or indirect injuries that destroy lung parenchymal cells and dramatically reduce lung function. Although some research progress has been made in recent years, the pathogenesis of ALI/ARDS remains unclear due to its heterogeneity and etiology. MicroRNAs (miRNAs), a type of small noncoding RNA, play a vital role in various diseases. In ALI/ARDS, miRNAs can regulate inflammatory and immune responses by targeting specific molecules. Regulation of miRNA expression can reduce damage and promote the recovery of ALI/ARDS. Consequently, miRNAs are considered as potential diagnostic indicators and therapeutic targets of ALI/ARDS. Given that inflammation plays an important role in the pathogenesis of ALI/ARDS, we review the miRNAs involved in the inflammatory process of ALI/ARDS to provide new ideas for the pathogenesis, clinical diagnosis, and treatment of ALI/ARDS.
Galectin 1—A Key Player between Tissue Repair and Fibrosis
Authors: Hermenean Anca , Oatis Daniela , Herman Hildegard , Ciceu Alina , D’Amico Giovanbattista , Trotta Maria Consiglia
Published: May 2022

Galectins are ten family members of carbohydrate-binding proteins with a high affinity for β galactose-containing oligosaccharides. Galectin-1 (Gal-1) is the first protein discovered in the family, expressed in many sites under normal and pathological conditions. In the first part of the review article, we described recent advances in the Gal-1 modulatory role on wound healing, by focusing on the different phases triggered by Gal-1, such as inflammation, proliferation, tissue repair and re-epithelialization. On the contrary, Gal-1 persistent over-expression enhances angiogenesis and extracellular matrix (ECM) production via PI3K/Akt pathway activation and leads to keloid tissue. Therefore, the targeted Gal-1 modulation should be considered a method of choice to treat wound healing and avoid keloid formation. In the second part of the review article, we discuss studies clarifying the role of Gal-1 in the pathogenesis of proliferative diabetic retinopathy, liver, renal, pancreatic and pulmonary fibrosis. This evidence suggests that Gal-1 may become a biomarker for the diagnosis and prognosis of tissue fibrosis and a promising molecular target for the development of new and original therapeutic tools to treat fibrosis in different chronic diseases.
Verrucosidin Derivatives from the Deep Sea Cold-Seep-Derived Fungus Penicillium polonicum CS-252
Authors: Li Yanhe , Li Xiaoming , Li Xin , Yang Suiqun , Wang Bingui , Li Honglei
Published: May 2022

Six novel verrucosidin derivatives, namely, poloncosidins A–F (1–6), together with one known analogue (7), were isolated and identified from the deep-sea-derived fungus Penicillium polonicum CS-252, which was obtained from cold-seep sediments collected in the South China Sea at a depth of 1183 m. Their structures were mainly established on the basis of a detailed interpretation of NMR spectroscopic and mass spectrometric data. The relative and absolute configurations of compounds 1–6 were determined by ECD calculations and a DP4+ probability analysis. Compounds 1–5 represent the first examples of verrucosidins with a 2,5-dihydrofuran ring which is uncommon among the known analogues. These compounds exhibited inhibitory activities against several human and aquatic pathogens with MIC values ranging from 4 to 32 μg/mL.
Comprehensive Analysis of Oligo/Polysialylglycoconjugates in Cancer Cell Lines
Authors: Hane Masaya , Kitajima Ken , Sato Chihiro
Published: May 2022

In cancer cells, cell-surface sialylation is altered, including a change in oligo/polysialic acid (oligo/polySia) structures. Since they are unique and rarely expressed in normal cells, oligo/polySia structures may serve as promising novel biomarkers and targets for therapies. For the diagnosis and treatment of the disease, a precise understanding of the oligo/polySia structures in cancer cells is necessary. In this study, flow cytometric analysis and gene expression datasets were obtained from sixteen different cancer cell lines. These datasets demonstrated the ability to predict glycan structures and their sialylation status. Our results also revealed that sialylation patterns are unique to each cancer cell line. Thus, we can suggest promising combinations of antibody and cancer cell for glycan prediction. However, the precise prediction of minor glycans need to be further explored.
MYD88 Mutations: Transforming the Landscape of IgM Monoclonal Gammopathies
Authors: Alcoceba Miguel , García-Álvarez María , Medina Alejandro , Maldonado Rebeca , González-Calle Verónica , Chillón María Carmen , Sarasquete María Eugenia , González Marcos , García-Sanz Ramón , Jiménez Cristina
Published: May 2022

The MYD88 gene has a physiological role in the innate immune system. Somatic mutations in MYD88, including the most common L265P, have been associated with the development of certain types of lymphoma. MYD88L265P is present in more than 90% of patients with Waldenström’s macroglobulinemia (WM) and IgM monoclonal gammopathy of undetermined significance (IgM-MGUS). The absence of MYD88 mutations in WM patients has been associated with a higher risk of transformation into aggressive lymphoma, resistance to certain therapies (BTK inhibitors), and shorter overall survival. The MyD88 signaling pathway has also been used as a target for specific therapies. In this review, we summarize the clinical applications of MYD88 testing in the diagnosis, prognosis, follow-up, and treatment of patients. Although MYD88L265P is not specific to WM, few tumors present a single causative mutation in a recurrent position. The role of the oncogene in the pathogenesis of WM is still unclear, especially considering that the mutation can be found in normal B cells of patients, as recently reported. This may have important implications for early lymphoma detection in healthy elderly individuals and for the treatment response assessment based on a MYD88L265P analysis.
We Can Define the Domain of Information Online and Thus Globally Uniformly
Authors: Orthuber Wolfgang
Published: May 2022

Any information is (transported as) a selection from an ordered set, which is the “domain” of the information. For example, any piece of digital information is a number sequence that represents such a selection. Its senders and receivers (with software) should know the format and domain of the number sequence in a uniform way worldwide. So far, this is not guaranteed. However, it can be guaranteed after the introduction of the new “Domain Vector” (DV) data structure: “UL plus number sequence”. Thereby “UL” is a “Uniform Locator”, which is an efficient global pointer to the machine-readable online definition of the number sequence. The online definition can be adapted to the application so that the DV represents the application-specific, reproducible features in a precise (one-to-one), comparable, and globally searchable manner. The systematic, nestable online definition of domains of digital information (number sequences) and the globally defined DV data structure have great technical potential and are recommended as a central focus of future computer science.
Distribution of Phlebotomine Sand Flies (Diptera: Psychodidae) in the Lombardy Region, Northern Italy
Authors: Defilippo Francesco , Carrera Maya , Lelli Davide , Canziani Sabrina , Moreno Ana , Sozzi Enrica , Manarolla Giovanni , Chiari Mario , Marco Farioli , Cerioli Monica Pierangela , Lavazza Antonio
Published: May 2022

This study investigated the species composition and density of sand flies in the Lombardy region (Northern Italy). Sand flies were collected using CDC traps baited with CO2 (CO2–CDC traps) between June and August 2021. A total of 670 sand flies were collected. The specimens were identified as seven species belonging to two genera, Phlebotomus and Sergentomyia, namely, S. minuta, Ph. perniciosus, Ph. perfiliewii, Ph. neglectus, Ph. mascitti, Ph. papatasi, and Ph. ariasi. Phlebotomus perniciosus was the most abundant species (87.76%), followed by Ph. perfiliewii (7.31%), Ph. neglectus (3.13%), S. minuta (0.75%), Ph. mascitti (0.6%), Ph. papatasi (0.3%), and Ph. ariasi, for which only one specimen was identified. Among these identified species, five are considered vectors of Leishmania, which causes cutaneous and visceral leishmaniasis. As vector presence increases the risk of vector-borne leishmaniasis, these results suggest that Northern Italy could be a potential area of pathogen circulation over the next few years. These preliminary results suggest that the risk of borne leishmaniasis is high in this region of Northern Italy. Monitoring the distribution of sand fly species in areas suitable for their persistence is important for control programs aimed at reducing the risk of leishmaniasis infection.
Impact of S-Wave Amplitude in Right Precordial Leads on Improvement in Mitral Regurgitation following Cardiac Resynchronization Therapy
Authors: Kataoka Naoya , Imamura Teruhiko , Koi Takahisa , Tanaka Shuhei , Fukuda Nobuyuki , Ueno Hiroshi , Kinugawa Koichiro
Published: May 2022

Background: The therapeutic strategy for mitral regurgitation (MR) in patients with advanced heart failure and wide QRS complex who are indicated for both intervention to MR and cardiac resynchronization therapy (CRT), remains unclear. Objective: We aimed to determine electrocardiogram parameters that associate with MR reduction following CRT implantation. Methods: Among the patients with advanced heart failure and functional MR who intended to receive CRT implantation, baseline QRS morphology, electrical axis, PR interval, QRS duration, and averaged S-wave in right precordial leads (V1 to V3) in surface electrocardiogram were measured. The impact of these parameters on MR reduction following CRT implantation, which was defined as a reduction in MR ≥1 grade six months later, was investigated. Results: In 35 patients (median 71 years old, 18 men), 17 (49%) achieved an MR reduction following CRT implantation. Among baseline characteristics, only the higher S-wave amplitude in right precordial leads was an independent predictor of MR reduction (odds ratio 14.00, 95% confidence interval 1.65–119.00, p = 0.016) with a cutoff of 1.3 mV calculated through the area under the curve. The cutoff significantly stratified the cumulative incidences of heart failure re-admission and percutaneous mitral valve repair following CRT implantation (p = 0.032 and p = 0.011, respectively). Conclusions: In patients with advanced heart failure and functional MR, the baseline higher amplitude of S-wave in the right precordial leads might be a good indicator of MR improvement following CRT.
Thoracoscopic Implantation of Epicardial Left Ventricular Lead for Cardiac Resynchronization Therapy
Authors: Kim Hye Ree , Lim Kyunghee , Park Seung-Jung , Park Jong-Sung , Kim Ju Youn , Chung Suryeun , Jung Dong-Seop , Park Kyoung-Min , On Young Keun , Kim June Soo
Published: May 2022

(1) Background: Limited data exist on the safety and efficacy of epicardial left ventricular (LV) lead placement using video-assisted thoracoscopic surgery (VATS) for cardiac resynchronization therapy (CRT). (2) Methods: Acute and post-discharge outcomes of CRT were compared between patients with epicardial LV leads (Epicardial-LV group, n = 13) and those with endocardial LV leads (Endocardial-LV group, n = 243). (3) Results: Epicardial LV leads were implanted via VATS alone (n = 8) or along with mini-thoracotomy (n = 5), for failed endocardial implantation (n = 11) or recurrent lead dislodgement (n = 2). All epicardial procedures under general anesthesia with one-lung ventilation were successfully completed in 1.0 ± 0.4 h without phrenic nerve stimulation. LV pacing thresholds in the epicardial-LV (1.5 ± 1.0 V) and endocardial-LV (1.3 ± 0.8 V) were comparable (p = 0.651). All patients were discharged alive post-VATS 8.8 ± 3.9 days. During the follow-up (34.3 ± 28.6 months), all patients with epicardial LV leads stayed alive except for one cardiac death post-CRT 14 months and one heart transplantation post-CRT 30 months. All epicardial LV leads maintained stable performance without dislodgement/significant changes in pacing threshold/impedance. LV lead dislodgement occurred only in endocardial-LV (7/243, 2.9%). Efficacy in both groups was comparable in terms of QRS narrowing, increase in LV ejection fraction, and survival free of cardiac death, or heart-failure-related hospitalization. (4) Conclusions: Epicardial LV lead placement using VATS can be a safe and effective alternative to endocardial implantation, with comparable acute and post-discharge outcomes achieved by both approaches.