bluuebunny/crossref_metadata_2025 huggingface dataset:
A Tumor Homing Peptide-Linked Arsenic Compound Inhibits Pancreatic Cancer Growth and Enhances the Inhibitory Effect of Gemcitabine
Authors: He Hong , Dumesny Chelsea , Carrall Judith A. , Dillon Carolyn T. , de Roo Katja I. , Eutick Mal , Dong Li , Baldwin Graham S. , Nikfarjam Mehrdad
Published: October 2024

Arsenic trioxide (ATO) has been shown to inhibit pancreatic cancer (PC) cell growth in vitro and to promote the inhibitory effects of gemcitabine (Gem) on PC in vivo. However, the high toxicity of ATO associated with the required high doses and indiscriminate targeting has limited its clinical application. This study aimed to determine whether coupling arsenic to a tumor homing peptide would increase the inhibitory potency against PC cells. The effects of this peptide-linked arsenic compound (PhAs-LHP), the analogous non-targeting arsenic compound (phenylarsine oxide, PAO), and marketed ATO on PC growth were tested in vitro and in a mouse model. The data demonstrated that PhAs-LHP inhibited PC cell growth in vitro more potently, with IC50 values 10 times lower than ATO. Like ATO, PhAs-LHP induced cell death and cell cycle arrest. This cytotoxic effect of PhAs-LHP was mediated via a macropinocytosis-linked uptake pathway as amiloride (a macropinocytosis inhibitor) reduced the inhibitory effect of PhAs-LHP. More importantly, PhAs-LHP inhibited PC growth in mice and enhanced the inhibitory effect of Gem on PC growth at 2 times lower molar concentration than PAO. These results indicate that PhAs-LHP inhibited PC more potently than ATO/PAO and suggest a potential clinical use for the combination of Gem with the peptide-linked arsenic compound for the treatment of pancreatic cancer.
Adunctin E from Conamomum rubidum Induces Apoptosis in Lung Cancer via HSP90AA1 Modulation: A Network Pharmacology and In Vitro Study
Authors: Iksen Iksen , Singharajkomron Natsaranyatron , Nguyen Hien Minh , Hoang Hanh Nhu Thi , Ho Duc Viet , Pongrakhananon Varisa
Published: October 2024

Lung cancer stands out as a leading cause of death among various cancer types, highlighting the urgent need for effective anticancer drugs and the discovery of new compounds with potent therapeutic properties. Natural sources, such as the Conamomum genus, offer various bioactive compounds. Adunctin E (AE), a dihydrochalcone derived from Conamomum rubidum, exhibited several pharmacological activities, and its potential as an anticancer agent remains largely unexplored. Thus, this study aimed to elucidate its apoptotic-inducing effect and identify its molecular targets. The network pharmacology analysis led to the identification of 71 potential targets of AE against lung cancer. Subsequent gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome pathway enrichment analyses revealed the involvement of these targets in cancer-associated signaling pathways. Notably, HSP90AA1, MAPK1, and PIK3CA emerged as key players in apoptosis. In silico molecular docking and dynamic simulations suggested a strong and stable interaction between AE and HSP90AA1. In vitro experiments further confirmed a significant apoptotic-inducing effect of AE on lung cancer cell lines A549 and H460. Furthermore, immunoblot analysis exhibited a substantial decrease in HSP90AA1 levels in response to AE treatment. These findings support the potential anticancer activity of AE through the HSP90AA1 mechanism, underscoring its promise as a novel compound worthy of further research and development for anti-lung cancer therapy.
Impact of Digital Innovations on Health Literacy Applied to Patients with Special Needs: A Systematic Review
Authors: Cardoso Lucilene Bustilho , Couto Patrícia , Correia Patrícia , Lopes Pedro C. , Fernandes Juliana Campos Hasse , Fernandes Gustavo Vicentis Oliveira , Veiga Nélio Jorge
Published: October 2024

MHealth strategies have been used in various health areas, and mobile apps have been used in the context of health self-management. They can be considered an adjuvant intervention in oral health literacy, mainly for people with special health needs. Thus, the aim of this study was to identify the improvement of oral health literacy in patients with special needs when using digital platforms. A systematic literature review, based on the Joanna Briggs Institute (JBI) guidelines, was the main research method employed in this study. A search was undertaken in PubMed/MEDLINE and Cochrane Central Register of Controlled Trials (CENTRAL) databases, according to the relevant Mesh descriptors, their synonyms, and free terms (Entry Terms). Studies published between the years 2012 and 2023 were included. Two researchers independently assessed the quality of the included studies by completing the Newcastle–Ottawa Quality Assessment Scale questionnaire. The analysis corpus comprised 5 articles among the 402 articles selected after applying the inclusion/exclusion criteria (k = 0.97). The evidence from the considered articles is consensual regarding the effectiveness of using new technologies and innovations in promoting oral health literacy in patients with special health needs. The interventions were based on using the Illustration Reinforcement Communication System, inspired by the Picture Exchange Communication System, Nintendo® Wii™ TV, virtual reality, smartphones, with software applications to read messages sent, Audio Tactile Performance technique, and Art package. One study had a low-quality assessment, and four had a high quality. The evidence from the articles included in this systematic review is consistent regarding the effectiveness of using new technologies and innovations in promoting oral health literacy in patients with special health needs.
Endoscopic Ear Surgery, from the Last Ten to the Next Ten Years: A Critical Perspective
Authors: Alicandri-Ciufelli Matteo , Molinari Giulia , D’Alessandro Edoardo , Nocini Riccardo , Presutti Livio , Marchioni Daniele
Published: October 2024

Endoscopic ear surgery has gained increasing popularity starting from the early 2000s, introducing new anatomical, physiological and surgical concepts in ear and lateral skull base surgery. Its development has brought new scientific knowledge, thus improving surgical technique with a minimally invasive attitude. The aim of this perspective is to review and summarize all the steps that brought endoscopic ear surgery from a surgery practiced by a few teams to a worldwide recognized advancement in otology.
Unlocking the Potential of the Superficial Cervical Plexus Block in Chronic Pain Management: A Narrative Review and Single-Center, Retrospective Case Series
Authors: Zako Joe , Perez Jordi
Published: October 2024

Background/Objectives: The anesthetic block of the sensory branches of the superficial cervical plexus renders a specific area of the face, head, and anterior neck insensible and painless. Chronic pain in these areas can be difficult to diagnose and treat. In this report, we briefly review the existing evidence on the topic of the superficial cervical plexus block (SCPB) to set the context for our research. We then share our own clinical experience with the SCPB for managing chronic pain syndromes from both cancerous and non-cancerous etiologies. Methods: We first performed a comprehensive literature search and narrative review of clinical cases and studies that utilized the SCPB as an analgesic technique. We then conducted a retrospective case series of all patients who had received an SCPB at our pain clinic since 2020. Results: Our literature review found only a few cases reported, with most of them focusing on acute painful emergencies and perioperative pain syndromes and only very few addressing chronic pain. In our pain clinic, 14 patients received one or more SCPBs for chronic pain management. In 43% of these cases, the pain was related to cancer. The most common areas of pain corresponded to the regions supplied by the transverse cervical and great auricular nerves. The procedures were uneventful in all cases, and patients rated them as effective and worthwhile 71% of the time. Conclusions: Despite the lack of high-quality studies on SCPBs in pain management, the authors’ experience suggests that it is a valid minimally invasive alternative for managing chronic face, head, and neck pain.
Management of a Rare Case of Superior Mesenteric Artery Aneurysm Associated with a Pancreatic Cyst Complicated by Acute Rupture: A Case Report and Review of Literature
Authors: Dragulescu Petru Razvan , Geana (Anghel) Roxana Carmen , Robu Mircea , Nayyerani Reza , Dumitrescu Cristian , Dragan Anca , Vasilescu Catalin , Iliescu Vlad Anton , Stiru Ovidiu
Published: October 2024

Superior mesenteric artery aneurysms are a rare pathology, and rupture due to a pancreatic cyst in the context of alcohol-induced pancreatitis is an even rarer condition. The first line of treatment is usually an endovascular approach. We present the case of a 51-year-old male with alcohol-induced pancreatitis, diagnosed with a superior mesenteric artery aneurysm with active bleeding in close contact with a large pancreatic cyst. A covered stent was used to treat this condition. The patient developed hemorrhagic shock 12 h after the procedure and an urgent laparotomy was performed. A second rupture of the arterial wall at the distal end of the stent was observed and in order to obtain distal perfusion, first, an infrarenal aorta to superior mesenteric artery bypass distal to the rupture was performed in order to exclude the aneurysm. Secondly, a bypass originating from the distal end of the first bypass to the distal end of the superior mesenteric artery was performed. The patient had an uneventful recovery and was discharged after 10 days. We reviewed the literature regarding the incidence and the therapeutic management of superior mesenteric artery aneurysm complicated by pancreatic cyst. An advanced search on PubMed from 2004 to 2024 returned 194 results and after applying the inclusion–exclusion criteria, 11 publications were selected. Although the endovascular approach is usually the first line of treatment with obvious advantages for the patient, a patient-tailored approach should be made in such cases and surgery could be the first option, when considering that the mechanism of aneurysm rupture is due to erosion of the arterial wall by the pancreatic enzymes. Surgery has the advantage of cyst drainage and aneurysm exclusion and in our case proved lifesaving.
Clinical Efficacy and Safety of Ultrasound-Guided Injection with Low-Molecular-Weight Peptides from Hydrolyzed Collagen in Patients with Partial Supraspinatus Tendon Tears: A Pilot Study
Authors: Latini Luca , Porta Francesco , Maccarrone Vincenzo , Zompa Davide , Cipolletta Edoardo , Mirza Riccardo Mashadi , Filippucci Emilio , Vreju Florentin Ananu
Published: October 2024

Background: This study evaluates the clinical efficacy and safety of two ultrasound (US)-guided injections of a 5 mg/1 mL low-molecular-weight peptide (LWP) solution derived from hydrolyzed bovine collagen in patients with supraspinatus partial tendon tears. Methods: A total of 21 patients with symptomatic partial tears of the supraspinatus tendon, detected by US, were consecutively enrolled and received one injection at a baseline visit (T0) and one after two weeks (T1). The primary outcome measure was the visual analogue scale (VAS) for pain. Secondary outcomes were the shoulder pain and disability index (SPADI) total score and the safety of LWP injections. Patients were examined at baseline (T0), at a week 2 follow-up visit (T1), and at a week 12 follow-up visit (T2). Results: A statistically significant improvement was found for both VAS pain and SPADI total scores, between T0 and T2 visits. US-guided injections were well tolerated and, apart from one patient with a progression of a tendon tear, no adverse events were recorded. Conclusions: Intratendinous tear US-guided injection therapy with an LWP solution was found to be safe and effective in improving both pain and shoulder function at a 12-week follow-up visit. The present pilot study should be considered the first step justifying a larger confirmatory investigation.
CFD Modeling of HBI/scrap Melting in Industrial EAF and the Impact of Charge Layering on Melting Performance
Authors: Ugarte Orlando , Li Jianghua , Haeberle Jeff , Frasz Thomas , Okosun Tyamo , Zhou Chenn Q.
Published: October 2024

The melting of scrap and hot briquetted iron (HBI) in an AC electric arc furnace (EAF) is simulated by an advanced 3D computational fluid dynamics (CFD) model that captures the arc heating, the scrap/HBI melting process, and the solid collapse mechanisms. The CFD model is used to simulate a scenario where charge layering and EAF power profiles are provided by a real EAF operation. CFD simulation of the EAF operation shows proper prediction of the charge melting when compared with standard industry practice. Namely, the CFD model predicts a 32.5%/67.5% ratio of solid/liquid steel at the beginning of refining, which approaches the 30%/70% ratio used in standard practice. Based on this prediction, the melting rate in the CFD results differs by 8.3% from actual EAF operation. The impact of charge layering on melting is also investigated. CFD results show that distributing charge material into a greater number of layers in the first bucket (10 layers as compared to 4) enhances the melting rate by 12%. However, including dense material at the bottom of the furnace deteriorates melting performance, reducing the impact of the number of layers of the charge. The CFD platform can be used to optimize the use of HBI/scrap in real EAF operations and to determine best recipe practices.
Analysis of Interface Bonding Mechanism of Babbit Alloy/Steel Compound Castings with Different Surface Treatments
Authors: Song Heqian , Wang Mingjie , Zhang Dan , Zhang Guowei , Wen Kai , Zhang Yougui , Xia Jianqiang , Miao Xingang , Xu Hong
Published: October 2024

Tin-based Babbitt alloy/steel bimetallic castings were prepared by compound casting with different coating surface modification treatment. The defects of modified coating surface are effectively controlled, while the unmodified interface has a considerable number of micropores. In consequence, the additional composite surface modifier greatly increased the integrity of the bimetal interface and led to a significant improvement of shear strength to ~55.61 MPa, in comparison to its counterpart with no surface modifier of ~7.04 MPa. Based on extensive experimental investigation and theoretical analysis, the role of coating surface modifiers in the interfacial strengthening was revealed. Meanwhile, the diffusion behavior of the bimetal interface connection was analyzed by first-principles calculations. These results will shed new light on the understanding of the liquid–solid connection of bimetallic composites and improve defect control at the interface.
Flexible Composites with Rare-Earth Element Doped Polycrystalline Particles for Piezoelectric Nanogenerators
Authors: Fan Yanzhe , Jia Zihan , Zhang Zhuo , Gu Shengfei , Du Wenya , Lin Dabin
Published: October 2024

Energy harvesting plays an important role in advancing personalized wearables by enabling continuous monitoring, enhancing wearable functionality and facilitating sustainable solutions. We aimed to develop a flexible piezoelectric energy harvesting system based on inorganic piezoelectric materials that convert mechanical energy into electricity to power a wide range of mobile and portable electronic devices. There is significant interest in flexible piezoelectric energy harvesting systems that use inorganic piezoelectric materials due to their exceptional physical features and prospective applications. Herein, we successfully demonstrated a flexible piezoelectric nanogenerator (PENG) designed by the co-doped rare-earth element ceramics (RE-PMN-PT) embedded in PVDF and PDMS composite film and attained a significant output performance while avoiding electrical poling process. The impact of dielectric characteristics on the electrical output of nanogenerators was investigated, together with the structure of the composites. The Sm/La-PMN-PT particles effectively amplify both the voltage and current output, showcasing their potential to power portable and wearable devices, as demonstrated by their capacity to illuminate LEDs. The maximal output power of 2 mW was correlated with the high voltage (220 V) and current (90 µA) of Sm/La-PMN-PT/PVDF, which demonstrated that the device has the potential for energy harvesting in biomedical applications.
Development of a Quadruplex RT-qPCR for the Detection of Feline Kobuvirus, Feline Astrovirus, Feline Bufavirus, and Feline Rotavirus
Authors: Shi Kaichuang , He Mengyi , Long Feng , He Junxian , Yin Yanwen , Feng Shuping , Li Zongqiang
Published: October 2024

Feline kobuvirus (FeKoV), feline astrovirus (FeAstV), feline bufavirus (FeBuV), and feline rotavirus (FRV) are important pathogens for gastroenteritis, which is characterized by vomiting, diarrhea, and dehydration. Four pairs of primers and probes were designed to target the FeKoV VP1, FeAstV ORF2, FeBuV VP2, and FRV NSP4 genes, and a quadruplex real-time quantitative RT-PCR (RT-qPCR) assay capable of the simultaneous detection of four feline enteroviruses was developed after optimization of reaction conditions. The established quadruplex RT-qPCR assay showed high specificity, sensitivity, and reproducibility. The assay could detect and discriminate FeKoV, FeAstV, FeBuV, and FRV, but not other feline-related pathogens. The limits of detection (LODs) of FeKoV, FeAstV, FeBuV, and FRV were 109.761, 115.834, 125.481, and 113.875 copies/reaction, respectively. The intra- and inter-assay coefficients of variation (CV) were 0.15–1.61% and 0.15–1.59%, respectively. In all, 1869 clinical samples from Guangxi province in Southern China were tested using the developed assay, and the positivity rates of FeKoV, FeAstV, FeBuV, and FRV were 1.93%, 9.36%, 0.32%, and 0.75%, respectively. These samples were also tested using reference assays, and the coincidence rates of the results between the developed and reference methods were 99.63% (FeKoV), 98.72% (FeAstV), 100% (FeBuV), and 100% (FRV), respectively. The results indicated that the developed assay could provide a new detection method for these four viruses associated with feline gastroenteritis.
The Indosinian Granitoids of the Songpan–Garze–West Kunlun Orogenic Belt, China: Distribution, Petrochemistry, and Tectonic Insights
Authors: Deng Shiqi , Wang Yang
Published: October 2024

During the Indosinian orogeny, the Songpan–Garze–West Kunlun orogenic belt experienced significant tectonic and magmatic activity, leading to the widespread emplacement of granitoid bodies. This study provides a detailed petrochemical and geochemical analysis of these granitoids, offering new insights into their tectonic settings and magmatic evolution. The granitoids of this belt are systematically categorized into arc calc-alkaline and arc tholeiitic granitoids (ACG and ATG), cordierite peraluminous and muscovite peraluminous granitoids (CPG and MPG), potassium calc-alkaline granitoids (KCG), and peralkaline granitoids (PAG) suites. ACG and ATG types dominate early magmatism (230–190 Ma), reflecting a convergent tectonic setting, while KCG and MPG types magmatism, respectively, emerged 10–20 Myr and 15–25 Myr later, during post-collisional extensional phases. Geochemical analyses show that ACG and ATG granitoids follow calcic and calc-alkalic trends, while KCG and MPG display alkalic characteristics. These findings align with the region’s tectonic transition from the closure of the Paleo–Tethys Ocean to Late Triassic transpressional deformation. This study enhances the understanding of granitoid petrogenesis and provides valuable implications for regional tectonic evolution and mineral exploration.
Effects of Hydrogen Dissociation During Gas Flooding on Formation of Metal Hydride Cluster Ions in Secondary Ion Mass Spectrometry
Authors: Ekar Jernej , Markelj Sabina , Mozetič Miran , Zaplotnik Rok , Kovač Janez
Published: October 2024

The application of hydrogen flooding was recently shown to be a simple and effective approach for improved layer differentiation and interface determination during secondary ion mass spectrometry (SIMS) depth profiling of thin films, as well as an approach with potential in the field of quantitative SIMS analyses. To study the effects of hydrogen further, flooding of H2 molecules was compared to reactions with atomic H on samples of pure metals and their alloys. H2 was introduced into the analytical chamber via a capillary, which was heated to approximately 2200 K to achieve dissociation. Dissociation of H2 up to 30% resulted in a significant increase in the intensity of the metal hydride cluster secondary ions originating from the metallic samples. Comparison of the time scales of possible processes provided insight into the mechanism of hydride cluster secondary ion formation. Cluster ions presumably form during the recombination of the atoms and molecules from the sample and atoms and molecules adsorbed from the gas. This process occurs on the surface or just above it during the sputtering process. These findings coincide with those of previous mechanistic and computational studies.
The Impact of Fermented Milk Products Containing Bifidobacterium longum BB536 on the Gut Environment: A Randomized Double-Blind Placebo-Controlled Trial
Authors: Ejima Ryuta , Mishima Riko , Sen Akira , Yamaguchi Kana , Mitsuyama Eri , Kaneko Hiroki , Kimura Madoka , Arai Satoshi , Muto Natsumi , Hiraku Akari , Kato Kumiko , Kuwano Yasuyuki , Maruyama Hiroshi , Nakamura Masahiko , Iwabuchi Noriyuki , Nakano Manabu , Odamaki Toshitaka , Tanaka Miyuki
Published: October 2024

Probiotics, particularly those native to the gut microbiota, have a profound influence on the gut environment. In this study, we conducted a randomized placebo-controlled, double-blind, parallel-group comparison trial to investigate the effects of Bifidobacterium longum BB536 (B. longum BB536) on the fecal microbiota and metabolite compositions in healthy individuals. We compared the effects of fermented milk produced solely with Streptococcus thermophiles and Lactobacillus bulgaricus (placebo group) and fermented milk supplemented with B. longum BB536 (BY group). Our findings revealed a significantly greater relative abundance of Faecalibacterium in the BY group than in the placebo group by the 3rd day, a trend that persisted until the end of the trial on the 17th day. Additionally, the BY group presented significantly increased concentrations of tryptophan (Trp), Indole-3-lactic acid, and Indole-3-aldehyde on the 17th day. A significant positive correlation was observed between the relative abundance of Faecalibacterium and the number of viable B. longum BB536 bacteria in the feces. The concentrations of Trp and Indole-3-acetic acid were also significantly correlated with the number of viable B. longum BB536 bacteria in the feces. Our results suggest that B. longum BB536 intake can modulate the gut microbiota and metabolite profiles, which are general indicators for monitoring the gut environment, potentially conferring health benefits to the host.
Dietary Patterns Associated with Body-Composition Phenotype in a Middle-Aged and Elderly Population: A Population-Based Cross-Sectional Study
Authors: Park Jong , Yang Narae , Kim Kirang
Published: October 2024

Background/Objectives: There is currently limited understanding of the intricate dynamics between fat and muscle mass and the potential effect of dietary patterns. This study aimed to investigate the effects of overall dietary patterns on four body composition phenotypes associated with central obesity and low muscle mass in the middle-aged and elderly population of Korea. Methods: We examined data from 6120 adults aged ≥ 50 years, collected during the 4th (2008–2009) and 5th (2010–2011) Korea National Health and Nutrition Examination Survey. Participants were classified into four groups according to their combined status of central obesity and low muscle mass: healthy control, central obesity, low muscle mass, and sarcopenic obesity. Using factor analysis on the twenty-two pre-defined food groups, we identified three distinct dietary patterns: the “Healthy dietary pattern”, “Convenience-based dietary pattern”, and “Alcohol and side dish pattern”. Multivariate analysis included potential confounders such as age, sex, place of residence, marital status, educational level, occupation, household income, and physical activity level. Results: Higher scores in the “Healthy dietary pattern” were associated with lower odds of sarcopenic obesity (OR = 0.61, 95% CI = 0.40–0.92, p for trend = 0.018). For the “Convenience-based dietary pattern”, individuals in the third tertile of the dietary pattern score showed a marginal association with low muscle mass (OR = 1.18, 95% CI = 0.99–1.41, p for trend = 0.056). The “Alcohol and side dish pattern” was linked to higher odds of central obesity (OR = 1.22, 95% CI = 1.03–1.44, p for trend = 0.016) and low muscle mass (OR = 1.21, 95% CI = 1.01–1.45, p for trend = 0.043). Conclusions: The drinking practice of consuming foods high in saturated fats and salt alongside alcoholic drinks may negatively affect fat accumulation and muscle composition among older adults. Adopting a healthy eating pattern characterized by grains, vegetables, legumes, fruits, fish, and seaweed could be notably advantageous for promoting and maintaining healthy body composition and optimal skeletal muscle health.
Use of Biomarkers in Nutrition Intervention Studies of Children: A Scoping Review
Authors: Pratapwar Megha P. , Sheth Heli J. , Ravi Anushree K. , Block Morgan L. , Korber Kiersten A. , Kepsel Andrea , Leimanis-Laurens Mara , Comstock Sarah S.
Published: October 2024

Obesity in youth is an increasingly prevalent public health concern worldwide. Lifestyle interventions aim to help participants establish healthy habits and reduce obesity-related disease risk by targeting physical activity and dietary habits. Most studies assess weight loss, but biomarkers may enable more rapid and comprehensive assessment of intervention success. This scoping review aims to synthesize the published literature on which biomarkers are assessed during interventions for pediatric obesity to inform future use. This review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. A literature search of five databases conducted in February 2022 returned 1579 unique and relevant articles published between 2006 and 2021. After screening titles, abstracts, and full text, four reviewers determined that 43 studies met eligibility requirements. Quality screening was conducted, and 97.7% of papers were of fair or good quality. Of the 43 studies, 47% reported measures of adipose-related signaling molecules inclusive of adipokines, 74% included insulin-related biomarkers, 63% reported lipid-related biomarkers, 40% reported proinflammatory cytokine biomarkers, 12% reported measures of skin and/or plasma carotenoids, 40% measured blood pressure, and 21% included liver enzymes. Sixty-seven percent of studies measured biomarkers in whole blood, 40% measured biomarkers in plasma, 56% measured biomarkers in serum, and 2% measured biomarkers in urine. This work summarizes the current use of biomarkers in lifestyle intervention studies enrolling children. These biomarkers could be clinically relevant for pediatric weight management interventions.
Bioactive Compounds and Their Chondroprotective Effects for Osteoarthritis Amelioration: A Focus on Nanotherapeutic Strategies, Epigenetic Modifications, and Gut Microbiota
Authors: Hridayanka Kota Sri Naga , Duttaroy Asim K. , Basak Sanjay
Published: October 2024

In degenerative joint disease like osteoarthritis (OA), bioactive compounds like resveratrol, epigallocatechin gallate, curcumin, and other polyphenols often target various signalling pathways, including NFκB, TGFβ, and Wnt/β-catenin by executing epigenetic-modifying activities. Epigenetic modulation can target genes of disease pathophysiology via histone modification, promoter DNA methylation, and non-coding RNA expression, some of which are directly involved in OA but have been less explored. OA patients often seek options that can improve the quality of their life in addition to existing treatment with nonsteroidal anti-inflammatory drugs (NSAIDs). Although bioactive and natural compounds exhibit therapeutic potential against OA, several disadvantages loom, like insolubility and poor bioavailability. Nanoformulated bioactive compounds promise a better way to alleviate OA since they also control systemic events, including metabolic, immunological, and inflammatory responses, by modulating host gut microbiota that can regulate OA pathogenesis. Recent data suggest gut dysbiosis in OA. However, limited evidence is available on the role of bioactive compounds as epigenetic and gut modulators in ameliorating OA. Moreover, it is not known whether the effects of polyphenolic bioactive compounds on gut microbial response are mediated by epigenetic modulatory activities in OA. This narrative review highlights the nanotherapeutic strategies utilizing bioactive compounds, reporting their effects on chondrocyte growth, metabolism, and epigenetic modifications in osteoarthritis amelioration.
Adolescent Health in Lebanon: Exploring Alcohol Use, Dietary Patterns, Mental Health, Physical Activity, and Smoking Using the Global School-Based Student Health Survey Approach
Authors: Hoteit Maha , Hallit Souheil , Al Rawas Hanaa , Amasha Jana , Kobeissi Fadia , Fayyad Rafik , Sacre Yonna , Tzenios Nikolaos
Published: October 2024

Adolescence is a critical period for establishing lifelong health behaviors; yet in Lebanon, limited data exist on the prevalence of risk factors among this demographic. Objective: This study aims to assess alcohol consumption, dietary habits, physical activity, mental health, and smoking behaviors among Lebanese adolescents aged 13–17 years, with a focus on gender and school-type differences. Methods: A cross-sectional analysis was conducted in Lebanon between March and July 2022 using the Global School-based Student Health Survey (GSHS) questionnaire. A representative sample of students from public and private schools participated in the survey, with key variables analyzed to identify significant patterns and disparities. Results: Our findings reveal that 6.3% of adolescents consumed alcohol, with males reporting a higher prevalence and earlier initiation (p = 0.003). Gender differences were evident in dietary habits, where males were more likely to consume sugary drinks (p = 0.04) and have consistent breakfast habits (p = 0.003). Adolescents from private schools exhibited distinct dietary behaviors, including lower milk consumption (p < 0.001) and higher fatty food intake (p = 0.008). Males were also more physically active and reported better mental health outcomes compared to females (p = 0.004). Smoking behaviors showed that males smoked more frequently, while private school students reported smoking less. No significant difference was observed in bullying experiences between genders or school types. Conclusions: The study highlights critical health behaviors among Lebanese adolescents, with significant variations by gender and school type. These findings underscore the need for targeted interventions to address the identified risk factors and promote healthier behaviors in this population.
Dementia Rehabilitation Training for General Practitioners and Practice Nurses: Does It Make a Difference?
Authors: Lee Den-Ching A. , Russell Grant , Haines Terry P. , Hill Keith D. , O’Connor Claire M. C. , Layton Natasha , Swaffer Kate , Long Marita , Devanny Catherine , Callisaya Michele L.
Published: October 2024

Background/Objectives: Rehabilitation helps reduce disability in dementia. The Australian National Dementia Action Plan identifies a gap in clear treatment pathways post-diagnosis, affecting the quality of life for those with dementia. This study assessed the impact of a one-day dementia training course and follow-up on GPs’ and practice nurses’ knowledge, attitudes, and confidence regarding dementia rehabilitation. Methods: The training, led by two experienced GPs and an academic physiotherapist, covered dementia diagnosis, allied health roles, care planning, and referrals. The follow-up involved applying the learnt material and completing a reflective task. Three longitudinal surveys (Dementia Knowledge Assessment Scale—DKAS, General Practitioners’ Attitudes and Confidence towards Dementia Survey—GPACS-D, and Dementia Rehabilitation Scale) and Likert-scale statements were conducted pre-course, post-course, and at four-month follow-up, alongside a focus group. Descriptive and regression analyses were applied to survey data, and content analysis was used for focus group data. Results: Seventeen participants (14 GPs, 3 nurses) completed the pre–post-course survey, with eight (6 GPs, 2 nurses) participating in follow-up and focus group discussions. Post-course, DKAS scores increased by 12.1%, GPACS-D by 10.1%, and the dementia rehabilitation scale by 9.4%. Likert-scale statements improved by 8–79%. At the four-month follow-up, there was a slight, non-significant decline in most measures. Focus groups highlighted the training’s impacts, useful components, barriers, and suggestions for improvement. Conclusion: Training GPs and practice nurses in dementia rehabilitation enhances knowledge, awareness, and confidence. Ongoing efforts are needed to sustain benefits and address referral barriers for better access to dementia rehabilitation services.
Nursing Interventions Related to the Need for Oxygenation in Severe COVID-19 Disease in Hospitalized Adults: A Retrospective Study
Authors: Santiago-González Nicolás , García-Hernández María de Lourdes , Cruz-Bello Patricia , Chaparro-Díaz Lorena , Rico-González María de Lourdes , Hernández-Ortega Yolanda , Santiago-Abundio Jesús
Published: October 2024

COVID-19 affects the respiratory system, reducing the oxygen saturation level, leading to hypoxemia and increasing the metabolic oxygenation need. Objective: To describe the nursing interventions related to the need for oxygenation in hospitalized adults with severe COVID-19 disease in the Intensive Care Unit. Method: This was an observational, retrospective and descriptive study in a population of 2205 patients with a convenience sample of n = 430 and based on the North American Nursing Diagnosis Association (NANDA), the Nursing Interventions Classification (NIC) and the Nursing Outcomes Classification (NOC). The analysis was performed with a non-parametric test to determine the association between the nursing interventions and the need for oxygenation. Results: The findings are aimed at improving nursing interventions with statistical associations as follow: oxygen therapy (p < 0.000), airway suctioning (p < 0.000), airway management (p = 0.029), invasive mechanical ventilation (p < 0.000) and non-invasive mechanical ventilation (p = 0.022). NOC taxonomy expected outcomes in ventilation, 34% (146), alteration in gas exchange, 33.7% (145), and respiratory status, 558.9% (253), were severely compromised. Conclusions: The nursing interventions to maintain the respiratory status are focused on airway care and oxygen therapy in order to increase the oxygen saturation level and decrease the severity of the need for oxygenation.
Investigation of the Antimicrobial Resistance of Important Pathogens Isolated from Poultry from 2015 to 2023 in the United States
Authors: Wang Asher T. , Tang Liya , Gao Andrew , Zhang Ethan , Huang Grace , Shen Justin , Jia Qian , Huang Zuyi
Published: October 2024

Foodborne pathogens cause around 47.8 million illnesses in the U.S. annually, with antimicrobial misuse in food production, particularly in poultry processing, contributing significantly to this public health challenge. Misuse of antimicrobials can contribute to antimicrobial resistance (AMR) and make the treatment of pathogens increasingly difficult. This emphasizes the need to investigate antimicrobial resistance in U.S. poultry. This study analyzes data from the NCBI Pathogen Isolates Browser (2015–2023) to explore the relationships between antimicrobial-resistant pathogens, AMR genes, and antimicrobials detected with resistance in pathogens isolated from chicken and turkey. Using principal component analysis and hierarchical clustering, we mapped and profiled regional and temporal patterns of antimicrobial resistance. Salmonella enterica was the most prevalent antimicrobial-resistant pathogen across both chicken and turkey, with notable outbreaks, particularly in the Northeast. Antimicrobial-resistant Campylobacter jejuni was more prevalent in chicken, particularly in California and Georgia, while Escherichia coli and Shigella were more prominent in turkey, with concentrated antimicrobial resistance in Texas for pathogen samples isolated from chicken. Resistance to tetracycline and streptomycin was widespread, with distinct regional clusters: antimicrobial resistance was concentrated in states like Minnesota for pathogens isolated from chicken, while AMR found in pathogens isolated from turkey was more evenly distributed across the Midwest. Key AMR genes, such as tet(A), mdsA, and mdsB, also followed similar patterns, peaking in 2019 and significantly declining by 2022. The observed decline in AMR cases may be linked to improved biosecurity measures and disruptions in detection due to the COVID-19 pandemic. This comprehensive study of antimicrobial resistance in U.S. poultry provides valuable insights into resistance trends, which provide useful information to inform targeted interventions and policies to mitigate AMR threats in the poultry production industry. For consumers, these findings emphasize the importance of proper food handling and cooking practices to reduce the risk of exposure to resistant pathogens. Regulatory authorities should focus on enforcing stricter antimicrobial usage policies and enhancing surveillance systems to sustain the reduction in AMR cases.
A New LC-MS/MS-Based Method for the Simultaneous Detection of α-Tocopherol and Its Long-Chain Metabolites in Plasma Samples Using Stable Isotope Dilution Analysis
Authors: Maxones Alexander , Beck Eva , Rimbach Gerald , Birringer Marc
Published: October 2024

Background: Our study presented a novel LC-MS/MS method for the simultaneous quantification of α-tocopherol (α-TOH) and its phase II metabolites, α-13′-COOH and α-13′-OH, in human serum using deuterium-labeled internal standards (d6-α-TOH, d6-α-13′-COOH, d6-α-13′-OH). Methods: The method addresses the analytical challenge posed by the significantly different concentration ranges of α-TOH (µmol/L) and its metabolites (nmol/L). Previous methods quantified these analytes separately, which caused an increase in workflow complexity. Results: Key features include the synthesis of stable isotope-labeled standards and the use of a pentafluorophenyl-based core-shell chromatography column for baseline separation of both α-TOH and its metabolites. Additionally, solid phase extraction (SPE) with a HybridSPE® material provides a streamlined sample preparation, enhancing analyte recovery and improving sensitivity. By utilizing deuterium-labeled standards, the method compensates for matrix effects and ion suppression. This new approach achieves precise and accurate measurements with limits of detection (LOD) and quantification (LOQ), similar to previous studies. Calibration, accuracy, and precision parameters align well with the existing literature. Conclusions: Our method offers significant advantages in the simultaneous analysis of tocopherol and its metabolites despite concentration differences spanning up to three orders of magnitude. In contrast to earlier studies, which required separate sample preparations and analytical techniques for tocopherol and its metabolites, our approach streamlines this process. The use of a solid-phase extraction procedure allows for parallel sample preparation. This not only enhances efficiency but also significantly accelerates pre-analytical workflows, making the method highly suitable for large-scale studies.
Gentiopicroside Attenuates Lithium/Pilocarpine-Induced Epilepsy Seizures by Down-Regulating NR2B/CaMKII/CREB and TLR4/NF-κB Signaling Pathways in the Hippocampus of Mice
Authors: Tian Miao-Miao , Liu Gang , Du Juan , Liu Yue , Wei Wei , Lan Xiao-Bing , Hai Dong-Mei , Ma Lin , Yu Jian-Qiang , Liu Ning
Published: October 2024

Background: Epilepsy is a prevalent and disabling neurological condition characterized by recurrent seizures. Approximately 50% of adults with active epilepsy have at least one comorbidity and they are at a greater risk of premature death than the general population. Gentiopicroside (Gent) is a primary component of Gentiana macrophylla Pall. that has been shown to have diverse pharmacological properties. However, its role in epileptic seizures in adult mice and its underlying mechanism of action remain obscure. We aimed to explore the anti-epileptic effect and mechanism of Gent on lithium/pilocarpine (Pilo)-induced epilepsy seizures in mice. Methods: In this study, we established a lithium/Pilo-induced epilepsy model, and Gent was first given to mice 30 min before Pilo administration. Then, we detected behavioral and histopathological changes through electrocorticographic (ECoG) measurements, Nissl staining, Fluoro-Jade B (FJB) staining, and immunohistochemical staining. We then used molecular biology techniques, such as Western blotting, quantitative polymerase chain reaction (qPCR) analysis, and the enzyme-linked immunosorbent assay (ELISA) to investigate the mechanisms of Gent in lithium/Pilo-induced epileptic seizures in mice and lipopolysaccharide (LPS)-induced inflammatory astrocytes. Results: We confirmed that Gent could prevent abnormal ECoG activity, behavioral changes, and neurodegeneration. Subsequently, we found Gent could downregulate the factors that could promote apoptosis (i.e., the NR2B/CaMKII/CREB signaling cascade) and neuroinflammatory-related factors (i.e., the TLR4/NF-κB signaling cascade). Conclusions: Gent could be a potential therapeutic agent for epilepsy, offering possibilities for both prevention and treatment. Our research establishes a preliminary experimental framework for ongoing studies into Gent’s efficacy as a treatment for epilepsy.
Multilayer Nanocarrier for the Codelivery of Interferons: A Promising Strategy for Biocompatible and Long-Acting Antiviral Treatment
Authors: Ramos Thelvia I. , Villacis-Aguirre Carlos A. , Sandoval Felipe Sandoval , Martin-Solano Sarah , Manrique-Suárez Viana , Rodríguez Hortensia , Santiago-Padilla Leandro , Debut Alexis , Gómez-Gaete Carolina , Arias Marbel Torres , Montesino Raquel , Lamazares Emilio , Cabezas Ignacio , Hugues Florence , Parra Natalie C. , Altamirano Claudia , Ramos Oliberto Sánchez , Santiago-Vispo Nelson , Toledo Jorge R.
Published: October 2024

Background: Interferons (IFNs) are cytokines involved in the immune response with a synergistic regulatory effect on the immune response. They are therapeutics for various viral and proliferative conditions, with proven safety and efficacy. Their clinical application is challenging due to the molecules’ size, degradation, and pharmacokinetics. We are working on new drug delivery systems that provide adequate therapeutic concentrations for these cytokines and prolong their half-life in the circulation, such as nanoformulations. Methods: Through nanoencapsulation using electrospray technology and biocompatible and biodegradable polymers, we are developing a controlled release system based on nanoparticles for viral infections of the respiratory tract. Results: We developed a controlled release system for viral respiratory tract infections. A prototype nanoparticle with a core was created, which hydrolyzed the polyvinylpyrrolidone (PVP) shell , releasing the active ingredients interferon-alpha (IFN-α) and interferon-gamma (IFN-γ). The chitosan (QS) core degraded slowly, with a controlled release of IFN-α. The primary and rapid effect of the interferon combination ensured an antiviral and immunoregulatory response from day one, induced by IFN-α and enhanced by IFN-γ. The multilayer design demonstrated an optimal toxicity profile. Conclusions: This formulation is an inhaled dry powder intended for the non-invasive intranasal route. The product does not require a cold chain and has the potential for self-administration in the face of emerging viral infections. This novel drug has applications in multiple infectious, oncological, and autoimmune conditions, and further development is proposed for its therapeutic potential. This prototype would ensure greater bioavailability, controlled release, fewer adverse effects, and robust biological action through the simultaneous action of both molecules.
Optimization of Plant Oxalate Quantification and Generation of Low-Oxalate Maize (Zea mays L.) through O7 Overexpression
Authors: Zhao Kai , Wang Tao , Zhao Bin-Bin , Yang Jun
Published: October 2024

Oxalate, the simplest dicarboxylic acid, is a prevalent antinutrient that chelates with various metals and can lead to the formation of kidney stones in humans. The accurate detection of the oxalate concentration in food and the cultivation of low-oxalate crops are important for enhancing public health. In this study, we established a high-throughput and highly sensitive technique for oxalate detection using ultra-high-performance liquid chromatographic–triple quadrupole tandem mass spectrometry (UPLC-QqQ-MS/MS). Additionally, we overexpressed the gene O7, which encodes oxalyl-CoA synthetase in the maize oxalate degradation pathway, resulting in O7-OE lines. By employing the UPLC-QqQ-MS/MS method to measure oxalate levels in these transgenic lines, we observed that the oxalate content in the kernels of O7-OE lines was reduced by approximately 43%, with a concurrent increase in some micronutrients such as zinc. Importantly, the transgenic maize showed normal seed storage compound accumulation or other agronomic characteristics. In summary, we developed a high-throughput detection method that advances oxalate measurement. Furthermore, by generating new maize germplasm with diminished oxalate, our work offers potential health advantages to consumers.
Assessment of Genetic Diversity and Population Structure of Exotic Sugar Beet (Beta vulgaris L.) Varieties Using Three Molecular Markers
Authors: Sun Bowei , Li Shengnan , Pi Zhi , Wu Zedong , Wang Ronghua
Published: October 2024

Sugar beet (Beta vulgaris L.) is a biennial herb belonging to the Amaranthaceae family. It contributes to approximately 30% of the world’s total sucrose production and is an economically important crop. In this study, we analyzed the genetic diversity and population structure of 132 exotic sugar beet varieties using three molecular makers: four pairs of simple sequence repeat (SSR) primers, three pairs of insertion–deletion sequence (InDel) primers, and 20 cis-element amplification polymorphism (CEAP) primers. The results indicated that the number of alleles (Na) was 298, among which the number of effective alleles (Ne) was 182.426 (accounting for approximately 61.2%). The mean value of the genetic diversity index was 0.836. The polymorphic information content (PIC) was 0.639–0.907 (mean = 0.819), indicating a high level of polymorphism. These sugar beet varieties were classified into six clusters using the UPGMA method of cluster analysis. Population structure analysis revealed that the most ideal K value was 6. This indicated that the test materials could be divided into six categories, consistent with the clustering results. The clustering results indicated that most sugar beet varieties from the same breeding company clustered together, and the genetic distance between them was small, indicating that they may share the same male and/or female parent. Some varieties from different companies clustered together, indicating a narrow genetic base and potential exchange of germplasm resources between breeding companies. This study revealed the genetic differences among exotic sugar beet varieties and characteristics of the population structure. It provided a scientific basis for the identification of sugar beet varieties and markers-assisted breeding in China in the future.