bluuebunny/crossref_metadata_2025 huggingface dataset:
The Anti-Oxidative and Anti-Neuroinflammatory Effects of Sargassum horneri by Heme Oxygenase-1 Induction in BV2 and HT22 Cells
Authors: Ko Wonmin , Lee Hwan , Kim Nayeon , Jo Hee Geun , Woo Eun-Rhan , Lee Kyounghoon , Han Young Seok , Park Sang Rul , Ahn Ginnae , Cheong Sun Hee , Lee Dong-Sung
Published: May 2021

Sargassum horneri is used as a traditional medicinal agent and exhibits various pharmacological effects. In this study, we found that the 70% EtOH extract contained 34.37 ± 0.75 μg/mg fucosterol. We tested the antioxidant activities of the 70% EtOH extracts and their fractions. The CH2Cl2-soluble fraction showed the strongest DPPH and ABTS radical scavenging activities. Next, we evaluated the anti-neuroinflammatory effects of S. horneri on lipopolysaccharide (LPS)-stimulated BV2 cells. Pretreatment with the extract and fractions suppressed LPS-induced production of nitric oxide (NO) in BV2 cells. The 70% EtOH, CH2Cl2-soluble fraction, and water-soluble fraction inhibited the production of prostaglandin E2, interleukin-6, and tumor necrosis factor-α, as well as markedly blocking LPS-induced expression of inducible NO synthase and cyclooxygenase-2 via inactivation of the nuclear factor-kappa B pathway. In addition, the CH2Cl2-soluble fraction showed the most remarkable heme oxygenase (HO)-1 expression effects and increased nuclear erythroid 2-related factor translocation in the nucleus. In HT22 cells, the CH2Cl2-soluble fraction inhibited cell damage and ROS production caused by glutamate via the regulation of HO-1. Therefore, CH2Cl2-soluble fractions of S. horneri can attenuate oxidative action and neuroinflammatory responses via HO-1 induction, demonstrating their potential in the treatment of neuroinflammatory diseases.
Genome-Wide Screening of Oxidizing Agent Resistance Genes in Escherichia coli
Authors: Chen Hao , Wilson Jessica , Ercanbrack Carson , Smith Hannah , Gan Qinglei , Fan Chenguang
Published: May 2021

The use of oxidizing agents is one of the most favorable approaches to kill bacteria in daily life. However, bacteria have been evolving to survive in the presence of different oxidizing agents. In this study, we aimed to obtain a comprehensive list of genes whose expression can make Escherichiacoli cells resistant to different oxidizing agents. For this purpose, we utilized the ASKA library and performed a genome-wide screening of ~4200 E. coli genes. Hydrogen peroxide (H2O2) and hypochlorite (HOCl) were tested as representative oxidizing agents in this study. To further validate our screening results, we used different E. coli strains as host cells to express or inactivate selected resistance genes individually. More than 100 genes obtained in this screening were not known to associate with oxidative stress responses before. Thus, this study is expected to facilitate both basic studies on oxidative stress and the development of antibacterial agents.
Hydrodynamical and Hydrochemical Assessment of Pumped-Storage Hydropower (PSH) Using an Open Pit: The Case of Obourg Chalk Quarry in Belgium
Authors: Poulain Angélique , Pujades Estanislao , Goderniaux Pascal
Published: May 2021

Pumped storage hydropower (PSH) enables the temporary storage of energy, including from intermittent renewable sources, and provides answers to the difficulties related to the mismatch between supply and demand of electrical energy over time. Implementing a PSH station requires two reservoirs at different elevations and with large volumes of water. The idea of using old, flooded open-pit quarries as a lower reservoir has recently emerged. However, quarries cannot be considered as impervious reservoirs, and they are connected to the surrounding aquifers. As a result, PSH activities may entail environmental impacts. The alternation of the pumping–discharge cycles generates rapid and periodic hydraulic head fluctuations in the quarry, which propagate into the surrounding rock media forcing the exchange of water and inducing the aeration of groundwater. This aeration can destabilize the chemical balances between groundwater and minerals in the underground rock media. In this study, two numerical groundwater models based on the chalk quarry of Obourg (Belgium) were developed considering realistic pumping–discharge scenarios. The aim of these models was to investigate the hydrodynamic and hydrochemical impact of PSH activities on water inside the quarry and in the surrounding rock media. Results showed that (1) water exchanges between the quarry and the adjacent rock media have a significant influence on the hydraulic head, (2) the frequency of the pump–discharge scenarios influence the potential environmental impacts, and (3), in the case of chalk formations, the expected impact of PSH on the water chemical composition is relatively limited around the quarry. Results highlight that those hydrogeological and hydrochemical concerns should be assessed when developing a project of a PSH installation using a quarry as a lower reservoir, considering all particularities of the proposed sites.
Towards a New Learning Experience through a Mobile Application with Augmented Reality in Engineering Education
Authors: Criollo-C Santiago , Abad-Vásquez David , Martic-Nieto Marjan , Velásquez-G Fausto Andrés , Pérez-Medina Jorge-Luis , Luján-Mora Sergio
Published: May 2021

With the rise of information technology and digitization, education has been faced with the need to adopt new learning models using technology to create innovative educational methodologies. In addition, due to pandemic restrictions and in order to help contain the spread of the virus (COVID-19), all educational institutions have been forced to switch immediately to online education. The application of augmented reality (AR) in education provides important benefits, such as increased engagement and interactivity, and can help to minimize the negative effects of the disruption of face-to-face education. Therefore, this paper focuses on describing the effect of an augmented reality mobile application (NetAR) that was developed for engineering students as a complement to traditional education. To achieve this objective, an experimental group and a control group were established to work with the application for three weeks for three hours a day. Moreover, there are a number of usability issues with AR that may impact learning effectiveness and motivation. Therefore, the usability of the application was evaluated with the IBM Computer System Usability Questionnaire (CSUQ) tool. The usability results show that users are satisfied with NetAR, and the statistical data from the control group indicate that the application positively affects learning.
Computational Modeling of Ultrasound C-Scan Imaging Using Transmitted Signal Peak Density
Authors: Paul Koushik , Stromer Jeremy , Razmi Samuel , Pockaj Barbara A. , Ladani Leila , Razmi Jafar
Published: May 2021

Ultrasound measurement is a relatively inexpensive and commonly used imaging tool in the health sector. The through-transmission process of ultrasound measurement has been extensively evaluated for detecting abnormalities in tissue pathology. Compared to standard imaging parameters such as amplitude and time of flight, quantitative ultrasound parameters in the frequency domain can provide additional details regarding tissue microstructures. In this study, pressure magnitude or amplitude variation in the frequency spectrum of the received signal was evaluated as a potential imaging technique using the spectral peak density parameter. Computational C-scan imaging analysis was developed through a finite element model. The magnitude variation in the received signal showed different patterns while interacting with and without inclusions. Images were reconstructed based on peak density values that varied with the presence of solid structure. The computational results were verified with the experimental C-scan imaging results from the literature. It was found that magnitude variation can be an effective parameter for C-scan imaging of thin structures. The feasibility of the study was further extended to identify the structure’s relative position along with the sample depth during C-scan imaging. While moving the structure in the direction of the sample depth, the pressure magnitude variation strongly followed a second-degree polynomial trend.
Impacts of Traverse Speed and Material Thickness on Abrasive Waterjet Contour Cutting of Austenitic Stainless Steel AISI 304L
Authors: Llanto Jennifer Milaor , Tolouei-Rad Majid , Vafadar Ana , Aamir Muhammad
Published: May 2021

Abrasive water jet machining is a proficient alternative for cutting difficult-to-machine materials with complex geometries, such as austenitic stainless steel 304L (AISI304L). However, due to differences in machining responses for varied material conditions, the abrasive waterjet machining experiences challenges including kerf geometric inaccuracy and low material removal rate. In this study, an abrasive waterjet machining is employed to perform contour cutting of different profiles to investigate the impacts of traverse speed and material thickness in achieving lower kerf taper angle and higher material removal rate. Based on experimental investigation, a trend of decreasing the level of traverse speed and material thickness that results in minimum kerf taper angle values of 0.825° for machining curvature profile and 0.916° for line profiles has been observed. In addition, higher traverse speed and material thickness achieved higher material removal rate in cutting different curvature radii and lengths in line profiles with obtained values of 769.50 mm3/min and 751.5 mm3/min, accordingly. The analysis of variance revealed that material thickness had a significant impact on kerf taper angle and material removal rate, contributing within the range of 69–91% and 62–69%, respectively. In contrast, traverse speed was the least factor measuring within the range of 5–18% for kerf taper angle and 27–36% for material removal rate.
A Cross-Sectional Review of Blockchain in Thailand: Research Literature, Education Courses, and Industry Projects
Authors: Pongnumkul Suporn , Bunditlurdruk Thachaparn , Chaovalit Pimwadee , Tharatipyakul Atima
Published: May 2021

Blockchain technology, a decentralized database that encourages collaboration, transparency, and security, is popular in Thailand, as seen from many practical projects. Nevertheless, information about Thailand’s blockchain ecosystem is incomplete. We conducted literature reviews of research articles, education courses, and industry projects, to explore blockchain from the technology adoption flow including academic research, knowledge acquisition, and project implementation. Blockchain research articles were collected from the Scopus database, while projects and courses were retrieved from an internet search. After applying inclusion and exclusion criteria, a total of 13 research articles, 50 courses, and 54 industry projects, dated from 2016 to 2020, were included in our review. Based on an analysis with respect to trends, focuses, and characteristics in all three aspects, we found that blockchain technology in Thailand was in its infancy, but has been increasing in numbers and domain varieties. However, blockchain technology did not move towards the same direction for all aspects. Most research articles were found in government services, supply chain, and traceability domains; while most projects were in the financial domains. We offer possible explanations for this observation. This paper provides challenges and opportunities for the research community and involved parties in practical implementation.
Environmental Remediation of Metribuzin Herbicide by Mesoporous Carbon—Rich from Wheat Straw
Authors: Cara Irina Gabriela , Filip Manuela , Bulgariu Laura , Raus Lucian , Topa Denis , Jitareanu Gerard
Published: May 2021

Contemporary farming practices and rapid industrialization over the last few decades, have raised significant soil and water pollution with extreme toxic effects to humans and ecosystems. The widespread and inefficient use of pesticides, which surpass the soil’s self purification capability, has accelerated soil pollution. In this study, wheat straw biochar was obtained using the traditional pyrolysis technique and its characterization; in addition, the adsorption efficiency of metribuzin was investigated. Biochars’ physical and chemical characteristics were qualified using scanning electron microscopy and Fourier transform infrared spectroscopy. A batch sorption test and liquid chromatography coupled with mass spectrometry were also used to assess the biochar efficiency. SEM and FTIR confirmed the highly reactive surfaces of biochar, establishing efficient biomass conversion in low-oxygen conditions. The adsorption process showed best fit with pseudo second-order kinetic and Langmuir models, suggesting a chemisorption procedure and monolayer-type removal. Regarding its environmental and agricultural application, wheat straw biochar can be advanced as a recommendation solution for further research, which is fundamental for soil rehabilitation and the immobilization of contaminations.
A Traceable and Verifiable Tobacco Products Logistics System with GPS and RFID Technologies
Authors: Chen Chin-Ling , Lim Zi-Yi , Liao Hsien-Chou , Deng Yong-Yuan , Chen Peizhi
Published: May 2021

Tobacco products are an addictive commodity. According to the World Health Organization’s (WHO) latest statistics data, tobacco kills more than eight million people each year. In 2003, the WHO proposed the Framework Convention on Tobacco Control (FCTC) to provide an effective framework for the control of tobacco products to governments around the world. In the field of tobacco products, the hardest problem is how to prevent counterfeit tobacco products and smuggling. To solve the problems, we proposed a blockchain-based traceable and verifiable logistics system for tobacco products with global positioning system (GPS) and radio-frequency identification (RFID) Technologies. In this research, we provide an overview of system architecture, and also define the protocol and the smart contract in every phase that stores data into the blockchain center. We realized a decentralized database and authentication system that uses blockchain and smart contract technology; every protocol in every phase was designed to achieve the integrity of data and non-repudiation of message. Every tobacco product’s shipping record will be completed by scanning the RFID tag and retrieving the GPS with a mobile reader, where the record will be updated and validated in the blockchain center. In the end, the security and costs of the system were analyzed, and a comparison was made with the EU’s (European Commission) method. Our system is more flexible for transportation, more secure in the communication protocol, and more difficult to tamper and forge data. In general, the proposed scheme solved the problem of tobacco products counterfeiting and tracking issues.
Systematic Analysis of Micro-Fiber Thermal Insulations from a Thermal Properties Point of View
Authors: Lakatos Ákos , Csarnovics István , Csík Attila
Published: May 2021

In the European Union, almost 40% of all energy consumption comes from buildings, while another 20–25% comes from transport. In the European Union, including Hungary, only buildings with almost-zero energy demand could be built after 2020, and the use of renewable energies must be strengthened. The Renewable Directive stipulated that by 2020, the share of renewable energy in buildings must be 25%, and in transport it must be 10%; the use of electric vehicles is vital. There are about four million dwellings in Hungary, of which approximately three million need to be renovated, and only some of these (a few hundred) meet the cost-optimized level of the 2020 directive. The use of insulation materials is very important in the transport sector, too. Insulation materials are also used by aircraft and electric vehicles. To reduce the energy loss from buildings, different insulation materials can be used; investigations of insulation materials are very important. This paper presents a comprehensive research report on insulation materials which could be used for building elements, HVAC (heating, ventilation, and air conditioning appliances) equipment, and vehicles. In this paper, laboratory investigations will be presented along with calculations to better understand the properties and behavior of these materials. For this, firstly structural analysis with scanning electron microscope will be presented. Moreover, the paper will present thermal conductivity and combustion heat measurement results. The sorption and hydrophobic behavior of the materials will be also revealed. Finally, the article will also display differential scanning calorimetry measurements and Raman spectroscopy results of the samples. The research was conducted on four different types of colorized microfiber lightweight wool insulation.
Reproduction in Trypanosomatids: Past and Present
Authors: Gutiérrez-Corbo Camino , Domínguez-Asenjo Bárbara , Martínez-Valladares María , Pérez-Pertejo Yolanda , García-Estrada Carlos , Balaña-Fouce Rafael , Reguera Rosa M.
Published: May 2021

Diseases caused by trypanosomatids (Sleeping sickness, Chagas disease, and leishmaniasis) are a serious public health concern in low-income endemic countries. These diseases are produced by single-celled parasites with a diploid genome (although aneuploidy is frequent) organized in pairs of non-condensable chromosomes. To explain the way they reproduce through the analysis of natural populations, the theory of strict clonal propagation of these microorganisms was taken as a rule at the beginning of the studies, since it partially justified their genomic stability. However, numerous experimental works provide evidence of sexual reproduction, thus explaining certain naturally occurring events that link the number of meiosis per mitosis and the frequency of mating. Recent techniques have demonstrated genetic exchange between individuals of the same species under laboratory conditions, as well as the expression of meiosis specific genes. The current debate focuses on the frequency of genomic recombination events and its impact on the natural parasite population structure. This paper reviews the results and techniques used to demonstrate the existence of sex in trypanosomatids, the inheritance of kinetoplast DNA (maxi- and minicircles), the impact of genetic exchange in these parasites, and how it can contribute to the phenotypic diversity of natural populations.
Functional Attributes of Myco-Synthesized Silver Nanoparticles from Endophytic Fungi: A New Implication in Biomedical Applications
Authors: Seetharaman Prabu Kumar , Chandrasekaran Rajkuberan , Periakaruppan Rajiv , Gnanasekar Sathishkumar , Sivaperumal Sivaramakrishnan , Abd-Elsalam Kamel A. , Valis Martin , Kuca Kamil
Published: May 2021

To develop a benign nanomaterial from biogenic sources, we have attempted to formulate and fabricate silver nanoparticles synthesized from the culture filtrate of an endophytic fungus Penicillium oxalicum strain LA-1 (PoAgNPs). The synthesized PoAgNPs were exclusively characterized through UV–vis absorption spectroscopy, Fourier Transform Infra-Red spectroscopy (FT-IR), X-ray powder diffraction (XRD), and Transmission Electron Microscopy (TEM) with energy dispersive X-ray spectroscopy (EDX). The synthesized nanoparticles showed strong absorbance around 430 nm with surface plasmon resonance (SPR) and exhibited a face-centered cubic crystalline nature in XRD analysis. Proteins presented in the culture filtrate acted as reducing, capping, and stabilization agents to form PoAgNPs. TEM analysis revealed the generation of polydispersed spherical PoAgNPs with an average size of 52.26 nm. The PoAgNPs showed excellent antibacterial activity against bacterial pathogens. The PoAgNPs induced a dose-dependent cytotoxic activity against human adenocarcinoma breast cancer cell lines (MDA-MB-231), and apoptotic morphological changes were observed by dual staining. Additionally, PoAgNPs demonstrated better larvicidal activity against the larvae of Culex quinquefasciatus. Moreover, the hemolytic test indicated that the as-synthesized PoAgNPs are a safe and biocompatible nanomaterial with versatile bio-applications.
Parkinson’s Disease in Romania: A Scoping Review
Authors: Rosca Elena Cecilia , Tudor Raluca , Cornea Amalia , Simu Mihaela
Published: May 2021

Parkinson’s disease (PD) is a significant cause of disability, with a fast-growing prevalence. This review summarizes the epidemiological and clinical data, research on the diagnostic approaches and the interventions available in the Eastern European country of Romania. This scoping review follows the recommendations on the scoping review methodology by Joanna Briggs Institute. We searched four databases (up to 27 January 2021). The data of eligible studies were extracted in standardized forms. We identified 149 unique studies from 1133 records, with 11 epidemiological studies, 52 studies investigating clinical aspects of PD, 35 studies on diagnostic tools, and 51 intervention studies. A narrative synthesis is provided and placed in a historical context. Our review revealed a considerable increase in the Romanian research on PD in the latest 15 years, which largely follows international trends. However, we also identified several research gaps that provide useful information for policymakers, public health specialists, and clinicians.
Low-Intensity Focused Ultrasound-Mediated Attenuation of Acute Seizure Activity Based on EEG Brain Functional Connectivity
Authors: Zhang Minjian , Li Bo , Lv Xiaodong , Liu Sican , Liu Yafei , Tang Rongyu , Lang Yiran , Huang Qiang , He Jiping
Published: May 2021

(1) Background: Ultrasound has been used for noninvasive stimulation and is a promising technique for treating neurological diseases. Epilepsy is a common neurological disorder, that is attributed to uncontrollable abnormal neuronal hyperexcitability. Abnormal synchronized activities can be observed across multiple brain regions during a seizure. (2) Methods: we used low-intensity focused ultrasound (LIFU) to sonicate the brains of epileptic rats, analyzed the EEG functional brain network to explore the effect of LIFU on the epileptic brain network, and continued to explore the mechanism of ultrasound neuromodulation. LIFU was used in the hippocampus of epileptic rats in which a seizure was induced by kainic acid. (3) Results: By comparing the brain network characteristics before and after sonication, we found that LIFU significantly impacted the functional brain network, especially in the low-frequency band. The brain network connection strength across multiple brain regions significantly decreased after sonication compared to the connection strength in the control group. The brain network indicators (the path length, clustering coefficient, small-worldness, local efficiency and global efficiency) all changed significantly in the low-frequency. (4) Conclusions: These results revealed that LIFU could reduce the network connections of epilepsy circuits and change the structure of the brain network at the whole-brain level.
Synthetic Mechanism Studies of Iron Selenides: An Emerging Class of Materials for Electrocatalysis
Authors: Hou Bo , Benito-Alifonso David , Webster Richard F. , Cherns David , Galan M. Carmen , Fermín David J.
Published: May 2021

Solution-processed iron selenide nanocrystals (NCs) have recently attracted considerable attention in electrocatalysis water splitting. Nevertheless, a primary challenge in current iron-based NCs chemical synthesis is controlling phase purities between each chalcogen (monochalcogenide, dichalcogenides, and oxides), which requires a comprehensive understanding of the reaction mechanisms at the early stages of nucleation. Herein, we investigate the fundamental steps in transforming molecular organoiron and organoselenium precursors to iron selenides NCs with the view of developing universal synthesis protocols for phase pure metal selenium and metal oxides NCs. The main intermediate species and volatile by-products are identified by high-resolution electron microscopy and Nuclear Magnetic Resonance (NMR) spectroscopy (1H, 13C, and 31P). Experimental evidence suggests that the phase determining factor is the coordinating reactivity difference between olefins (1-octadecene, oleylamine), tributylphosphine and trioctylphosphine associated with their corresponding Se bond cleavage. This work proposes organoselenium interconversion reaction mechanisms during iron selenides synthesis, offering a universal synthetic strategy for other electrocatalytically or photocatalytically active layered metal selenides materials.
Exosome-Based Molecular Transfer Activity of Macrophage-Like Cells Involves Viability of Oral Carcinoma Cells: Size Exclusion Chromatography and Concentration Filter Method
Authors: Lu Yanyin , Eguchi Takanori , Sogawa Chiharu , Taha Eman A. , Tran Manh Tien , Nara Toshiki , Wei Penggong , Fukuoka Shiro , Miyawaki Takuya , Okamoto Kuniaki
Published: May 2021

Extracellular vesicles (EV) heterogeneity is a crucial issue in biology and medicine. In addition, tumor-associated macrophages are key components in cancer microenvironment and immunology. We developed a combination method of size exclusion chromatography and concentration filters (SEC-CF) and aimed to characterize different EV types by their size, cargo types, and functions. A human monocytic leukemia cell line THP-1 was differentiated to CD14-positive macrophage-like cells by stimulation with PMA (phorbol 12-myristate 13-acetate) but not M1 or M2 types. Using the SEC-CF method, the following five EV types were fractionated from the culture supernatant of macrophage-like cells: (i) rare large EVs (500–3000 nm) reminiscent of apoptosomes, (ii) EVs (100–500 nm) reminiscent of microvesicles (or microparticles), (iii) EVs (80–300 nm) containing CD9-positive large exosomes (EXO-L), (iv) EVs (20–200 nm) containing unidentified vesicles/particles, and (v) EVs (10–70 nm) containing CD63/HSP90-positive small exosomes (EXO-S) and particles. For a molecular transfer assay, we developed a THP-1-based stable cell line producing a GFP-fused palmitoylation signal (palmGFP) associated with the membrane. The THP1/palmGFP cells were differentiated into macrophages producing palmGFP-contained EVs. The macrophage/palmGFP-secreted EXO-S and EXO-L efficiently transferred the palmGFP to receiver human oral carcinoma cells (HSC-3/palmTomato), as compared to other EV types. In addition, the macrophage-secreted EXO-S and EXO-L significantly reduced the cell viability (ATP content) in oral carcinoma cells. Taken together, the SEC-CF method is useful for the purification of large and small exosomes with higher molecular transfer activities, enabling efficient molecular delivery to target cells.
Modeling Gastrointestinal Diseases Using Organoids to Understand Healing and Regenerative Processes
Authors: Ollivier Alexane , Mahe Maxime M. , Guasch Géraldine
Published: May 2021

The gastrointestinal tract is a continuous series of organs from the mouth to the esophagus, stomach, intestine and anus that allows digestion to occur. These organs are frequently associated with chronic stress and injury during life, subjecting these tissues to frequent regeneration and to the risk of developing disease-associated cancers. The possibility of generating human 3D culture systems, named organoids, that resemble histologically and functionally specific organs, has opened up potential applications in the analysis of the cellular and molecular mechanisms involved in epithelial wound healing and regenerative therapy. Here, we review how during normal development homeostasis takes place, and the role of the microenvironmental niche cells in the intestinal stem cell crypt as an example. Then, we introduce the notion of a perturbed niche during disease conditions affecting the esophageal–stomach junction and the colon, and describe the potential applications of organoid models in the analysis of human gastrointestinal disease mechanisms. Finally, we highlight the perspectives of organoid-based regenerative therapy to improve the repair of the epithelial barrier.
Children’s Beliefs about Pain: An Exploratory Analysis
Authors: Ives Lindsay T. , Stein Kate , Rivera-Cancel Alannah M. , Nicholas Julia K. , Caldwell Kristen , Datta Nandini , Mauro Christian , Egger Helen , Puffer Eve , Zucker Nancy L.
Published: May 2021

Functional abdominal pain (FAP) is one of the most common childhood medical complaints, associated with significant distress and impairment. Little is known about how children understand their pain. Do they attribute it to personal weakness? Do they perceive pain as having global impact, affecting a variety of activities? How do they cope with pain? We explored the pain beliefs of 5- to 9-year-old children with FAP using a novel Teddy Bear Interview task in which children answered questions about a Teddy bear’s pain. Responses were analyzed quantitatively and qualitatively. Results indicate that the majority of young children with FAP are optimistic about pain outcomes. Children generated many types of coping strategies for Teddy’s pain and adjusted their calibration of Teddy’s pain tolerance dependent on the activity being performed. Early warning signs also emerged: a subset of children were pessimistic about Teddy’s pain, and several children identified coping strategies that, while developmentally appropriate, could lead to excessive help seeking if not intervened upon (e.g., physician consultation and shot). The Teddy Bear Interview allows children to externalize their pain, making it a useful tool to access cognitive pain constructs in younger children. Thus, these findings highlight the importance of early intervention for childhood FAP.
Partners and Ex-Partners in Dreams: A Diary Study
Authors: Schredl Michael , Wood Lara C.
Published: May 2021

Romantic relationships are an important part of human life and thus, according to the continuity hypothesis of dreaming, one’s romantic partner should show up in dreams quite frequently. The present study is based on 1612 dream reports provided by 425 students. The findings confirmed the hypothesis that partner dreams are more frequent than ex-partner dreams and, thus, support the continuity hypothesis of dreaming. Moreover, interactions with ex-partners within the dream were more often negatively toned compared to dreamed interactions with the partner. Unexpectedly, we also found more positive emotions and friendliness in ex-partner dreams compared to partner dreams, indicating that partner dreams are more mundane. To conclude, dreams reflect important aspects of romantic partnerships and their break-ups and, thus, can be very helpful in psychotherapy.
Reflective Meta-Films with Anti-Damage Property via Field Distribution Manipulation
Authors: Yu Haichao , Tang Feng , Chen Jun , Yi Zao , Ye Xin , Wang Yiqun
Published: May 2021

The reflective optical multi-films with high damage thresholds are widely used in intense-light systems. Metasurfaces, which can manipulate light peculiarly, give a new approach to achieve highly reflective films by a single-layer configuration. In this study, reflective metasurfaces, composed of silicon nanoholes, are numerically investigated to achieve high damage thresholds. These nanoholes can confine the strongest electric field into the air zone, and, subsequently, the in-air electric field does not interact directly with silicon, attenuating the optothermal effect that causes damage. Firstly, the geometrical dependencies of silicon nanoholes’ reflectance and field distribution are investigated. Then, the excitation states of electric/magnetic dipoles in nanostructures are analyzed to explain the electromagnetic mechanism. Furthermore, the reflection dependences of the nanostructures on wavelength and incident angle are investigated. Finally, for a typical reflective meta-film, some optothermal simulations are conducted, in which a maximum laser density of 0.27 W/µm2 can be handled. The study provides an approach to improve the laser damage threshold of reflective nanofilms, which can be exploited in many intense-light applications.
Mechanical and Durability Properties of Aerated Concrete Incorporating Rice Husk Ash (RHA) as Partial Replacement of Cement
Authors: Ali Tariq , Saand Abdullah , Bangwar Daddan Khan , Buller Abdul Salam , Ahmed Zaheer
Published: May 2021

In today’s world, the implementation of industrial ecology for sustainable industrial development is a common practice in the field of engineering. This practice promotes the recycling of by-product wastes. One of those by-product wastes is rice husk ash. This paper describes an investigation into the effect of rice husk ash (RHA) as a partial replacement for cement, to produce lightweight, aerated concrete. Type I Portland cement, fine aggregate, and aluminum powder as an aerating agent were used in this study. The RHA was used in different replacement levels, i.e., RHA was used to replace cement at 0%, 2.5%, 5%, 7.5%, 10%, 12.5% and 15% by weight. Aluminum powder was added during mixing at 0.5% by weight of binder to obtain lightweight, aerated concrete. Test results are presented in terms of physical, mechanical, and durability aspects that include density, compressive strength, split tensile strength, and flexural strength of concrete cured at different curing regimes, i.e., 3, 7, 28, and 90 days along with corrosion analysis, and sulphate attack at 28 days of curing. The test results show that using 10% RHA as a partial replacement of cement in aerated concrete is beneficial in triggering the strength and durability properties of concrete.
Small RNA-Sequencing: Approaches and Considerations for miRNA Analysis
Authors: Benesova Sarka , Kubista Mikael , Valihrach Lukas
Published: May 2021

MicroRNAs (miRNAs) are a class of small RNA molecules that have an important regulatory role in multiple physiological and pathological processes. Their disease-specific profiles and presence in biofluids are properties that enable miRNAs to be employed as non-invasive biomarkers. In the past decades, several methods have been developed for miRNA analysis, including small RNA sequencing (RNA-seq). Small RNA-seq enables genome-wide profiling and analysis of known, as well as novel, miRNA variants. Moreover, its high sensitivity allows for profiling of low input samples such as liquid biopsies, which have now found applications in diagnostics and prognostics. Still, due to technical bias and the limited ability to capture the true miRNA representation, its potential remains unfulfilled. The introduction of many new small RNA-seq approaches that tried to minimize this bias, has led to the existence of the many small RNA-seq protocols seen today. Here, we review all current approaches to cDNA library construction used during the small RNA-seq workflow, with particular focus on their implementation in commercially available protocols. We provide an overview of each protocol and discuss their applicability. We also review recent benchmarking studies comparing each protocol’s performance and summarize the major conclusions that can be gathered from their usage. The result documents variable performance of the protocols and highlights their different applications in miRNA research. Taken together, our review provides a comprehensive overview of all the current small RNA-seq approaches, summarizes their strengths and weaknesses, and provides guidelines for their applications in miRNA research.
Effectiveness of EUS-Guided Fine-Needle Biopsy versus EUS-Guided Fine-Needle Aspiration: A Retrospective Analysis
Authors: Kuraoka Naosuke , Hashimoto Satoru , Matsui Shigeru , Terai Shuji
Published: May 2021

Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) for pancreatic tumors and subepithelial lesions (SEL) of the gastrointestinal tract are effective for histological diagnosis. There are also reports that tissue sampling is possible with a smaller number of punctures by EUS-guided fine-needle biopsy (EUS-FNB). In this study, we retrospectively compared the diagnostic abilities of EUS-FNA and EUS-FNB. We examined 130 patients who underwent EUS-FNA/EUS-FNB for pancreatic tumors and SEL from July 2018 to January 2021. None of the cases underwent rapid on-site evaluation. There were 94 and 36 cases in the EUS-FNA and EUS-FNB groups, respectively. The median tumor size in the EUS-FNB group was 30 mm, which was significantly larger than the EUS-FNA group (p = 0.02). In addition, transgastric puncture was significantly more common in the EUS-FNB group (p = 0.01). The EUS-FNA and EUS-FNB groups had a sensitivity of 82.9% and 91.7% and an accuracy rate of 85.1% and 91.7%, respectively. However, both procedures had a comparable diagnostic ability.
Investigating the Impact of the Bit Depth of Fluorescence-Stained Images on the Performance of Deep Learning-Based Nuclei Instance Segmentation
Authors: Mahbod Amirreza , Schaefer Gerald , Löw Christine , Dorffner Georg , Ecker Rupert , Ellinger Isabella
Published: May 2021

Nuclei instance segmentation can be considered as a key point in the computer-mediated analysis of histological fluorescence-stained (FS) images. Many computer-assisted approaches have been proposed for this task, and among them, supervised deep learning (DL) methods deliver the best performances. An important criterion that can affect the DL-based nuclei instance segmentation performance of FS images is the utilised image bit depth, but to our knowledge, no study has been conducted so far to investigate this impact. In this work, we released a fully annotated FS histological image dataset of nuclei at different image magnifications and from five different mouse organs. Moreover, by different pre-processing techniques and using one of the state-of-the-art DL-based methods, we investigated the impact of image bit depth (i.e., eight bits vs. sixteen bits) on the nuclei instance segmentation performance. The results obtained from our dataset and another publicly available dataset showed very competitive nuclei instance segmentation performances for the models trained with 8 bit and 16 bit images. This suggested that processing 8 bit images is sufficient for nuclei instance segmentation of FS images in most cases. The dataset including the raw image patches, as well as the corresponding segmentation masks is publicly available in the published GitHub repository.
Assessment Practices and Beliefs: Teachers’ Perspectives on Assessment during Long Distance Learning
Authors: Ferretti Federica , Santi George Richard Paul , Del Zozzo Agnese , Garzetti Marzia , Bolondi Giorgio
Published: May 2021

The COVID-19 crisis has strongly affected the school system. In Italy, at-distance forms of didactics have been activated, changing the physiognomy of schools in terms of social interaction, practices and the identity of the individuals. In this paper, we address the issue of how teachers are facing the crisis: our focus is on assessment, as a key variable catalyzing personal history; beliefs; the interface between students; teachers and the school system. We study teachers’ beliefs as part of their identities and assessment as a fundamental variable of beliefs. A qualitative content analysis of the open-ended answers to an online questionnaire is carried out to understand the main characteristics associated with assessment by teachers and the obstacles to overcome in the context of long distance learning (LDL). The data show that teachers did not identify valid assessment methods for LDL during the lockdown, especially due to the lack of control over the students. A misconception emerges concerning the definition of formative assessment together with a new awareness of the possibilities offered by digital technologies regarding the individualization of didactics. This study helps to understand which teachers’ beliefs are related to assessment are and how they are shaped.
Pyrimidine-Based Push–Pull Systems with a New Anchoring Amide Group for Dye-Sensitized Solar Cells
Authors: Verbitskiy Egor V. , Steparuk Alexander S. , Zhilina Ekaterina F. , Emets Viktor V. , Grinberg Vitaly A. , Krivogina Ekaterina V. , Kozyukhin Sergey A. , Belova Ekaterina V. , Lazarenko Petr I. , Rusinov Gennady L. , Tameev Alexey R. , Nunzi Jean Michel , Charushin Valery N.
Published: May 2021

New donor–π–acceptor pyrimidine-based dyes comprising an amide moiety as an anchoring group have been designed. The dyes were synthesized by sequential procedures based on the microwave-assisted Suzuki cross-coupling and bromination reactions. The influence of the dye structure and length of π-linker on the photophysical and electrochemical properties and on the photovoltaic effectiveness of dye-sensitized solar cells was investigated. An increase in efficiency with a decrease in the length of π-linker was revealed. The D1 dye with only one 2,5-thienylene-linker provided the highest power conversion efficiency among the fabricated dye sensitized solar cells.
Single Step 2-Port Device De-Embedding Algorithm for Fixture-DUT-Fixture Network Assembly
Authors: Scafati Simone , Pellegrino Enza , de Paulis Francesco , Olivieri Carlo , Drewniak James , Orlandi Antonio
Published: May 2021

The de-embedding of measurement fixtures is relevant for an accurate experimental characterization of radio frequency and digital electronic devices. The standard technique consists in removing the effects of the measurement fixtures by the calculation of the transfer scattering parameters (T-parameters) from the available measured (or simulated) global scattering parameters (S-parameters). The standard de-embedding is achieved by a multiple steps process, involving the S-to-T and subsequent T-to-S parameter conversion. In a typical measurement setup, two fixtures are usually placed before and after the device under test (DUT) allowing the connection of the device to the calibrated vector network analyzer coaxial ports. An alternative method is proposed in this paper: it is based on the newly developed multi-network cascading algorithm. The matrices involved in the fixture-DUT-fixture cascading gives rise to a non-linear set of equations that is in one step analytically solved in closed form, obtaining a unique solution. The method is shown to be effective and at least as accurate as the standard multi-step de-embedding one.
Development and Real-Time Performance Evaluation of Energy Management Strategy for a Dynamic Positioning Hybrid Electric Marine Vessel
Authors: Bui Truong M. N. , Dinh Truong Q. , Marco James , Watts Chris
Published: May 2021

Hybridisation of energy sources in marine vessels has been recognized as one of the feasible solutions to improve fuel economy and achieve global emission reduction targets in the maritime sector. However, the overall performance of a hybrid vessel system is strongly dependent on the efficiency of the energy management system (EMS) that regulates the power-flow amongst the propulsion sources and the energy storage system (ESS). This study develops a simple but production-feasible and efficient EMS for a dynamic positioning (DP) hybrid electric marine vessel (HEMV) and real-time experimental evaluation within a hardware-in-the-loop (HIL) simulation environment. To support the development and evaluation, map-based performance models of HEMVs’ key components are developed. Control logics that underpin the EMS are then designed and verified. Real-time performance evaluation to assess the performance and applicability of the proposed EMS is conducted, showing the improvement over those of the conventional control strategies. The comparison using key performance indicators (KPIs) demonstrates that the proposed EMS could achieve up to 4.8% fuel saving per voyage, while the overall system performance remains unchanged as compared to that of the conventional vessel.
Phytotoxicity of Tires Evaluated in Simulated Conditions
Authors: Šourková Markéta , Adamcová Dana , Winkler Jan , Vaverková Magdalena Daria
Published: May 2021

Illegal dumps and landfills with disposed of tires are a fact of today, which should not be neglected as they represent a great ecological burden for the environment, affect the surrounding nature and disturb the landscape. This research was focused on testing the phytotoxicity of aqueous leachates from the fractions of tires in two sets of experiments—to simulate laboratory conditions (tire leaching in distilled water) and natural conditions (tire leaching in water from a recipient) using the Phytotoxkit testing kit (kit for the establishment of inhibition/stimulation effect on the root development) and the watercress test of phytotoxicity (biological method for the assessment of leachate phytotoxicity). Plants whose seeds were selected for the test were watercress (Lepidium sativum L.) and white mustard (Sinapis alba L.). The aqueous leachate was tested for 38 weeks. During the experiment, physical and chemical parameters were measured at intervals of 14 days by the testing instrument HACH TEST KIT: electric conductivity (EC), amount of dissolved oxygen (LDO) and pH. Results of root growth inhibition (IR) on the seeds of Lepidium sativum L. and Sinapis alba L. exhibited values ranging from 11.73% to 47.74% in the tested samples. Results of germination index (GI) on the seeds of Lepidium sativum L. exhibited values below 66% in the tested samples, which indicated the leachate phytotoxicity. In spite of the fact that similar studies are tackling the acute toxicity of leachates from tires (particularly to algae, embryos and animals), this research brings complementary information in testing the acute phytotoxicity of tire leachates to higher plants.
Characterization of the Fermentation and Sensory Profiles of Novel Yeast-Fermented Acid Whey Beverages
Authors: Luo Siyi (Rossie) , DeMarsh Timothy A. , deRiancho Dana , Stelick Alina , Alcaine Samuel D.
Published: May 2021

Acid whey is a by-product generated in large quantities during dairy processing, and is characterized by its low pH and high chemical oxygen demand. Due to a lack of reliable disposal pathways, acid whey currently presents a major sustainability challenge to the dairy industry. The study presented in this paper proposes a solution to this issue by transforming yogurt acid whey (YAW) into potentially palatable and marketable beverages through yeast fermentation. In this study, five prototypes were developed and fermented by Kluyveromyces marxianus, Brettanomyces bruxellensis, Brettanomyces claussenii, Saccharomyces cerevisiae (strain: Hornindal kveik), and IOC Be Fruits (IOCBF) S. cerevisiae, respectively. Their fermentation profiles were characterized by changes in density, pH, cell count, and concentrations of ethanol and organic acids. The prototypes were also evaluated on 26 sensory attributes, which were generated through a training session with 14 participants. While S. cerevisiae (IOCBF) underwent the fastest fermentation (8 days) and B. claussenii the slowest (21 days), K. marxianus and S. cerevisiae (Hornindal kveik) showed similar fermentation rates, finishing on day 20. The change in pH of the fermentate was similar for all five strains (from around 4.45 to between 4.25 and 4.31). Cell counts remained stable throughout the fermentation for all five strains (at around 6 log colony-forming units (CFU)/mL) except in the case of S. cerevisiae (Hornindal kveik), which ultimately decreased by 1.63 log CFU/mL. B. bruxellensis was the only strain unable to utilize all of the sugars in the substrate, with residual galactose remaining after fermentation. While both S. cerevisiae (IOCBF)- and B. claussenii-fermented samples were characterized by a fruity apple aroma, the former also had an aroma characteristic of lactic acid, dairy products, bakeries and yeast. A chemical odor characteristic of petroleum, gasoline or solvents, was perceived in samples fermented by B. bruxellensis and K. marxianus. An aroma of poorly aged or rancid cheese or milk also resulted from B. bruxellensis fermentation. In terms of appearance and mouthfeel, the S. cerevisiae (IOCBF)-fermented sample was rated the cloudiest, with the heaviest body. This study provides a toolkit for product development in a potential dairy-based category of fermented alcoholic beverages, which can increase revenue for the dairy industry by upcycling the common waste product YAW.