bluuebunny/crossref_metadata_2025 huggingface dataset:
Responses of Vegetation Phenology to Urbanization in Plateau Mountains in Yunnan, China
Authors: Sun Mengzhu , Yang Kun , Wang Jiasheng , Ran Wenjing , Rao Xun
Published: November 2023

The process of urbanization alters the distribution of land use and gives rise to certain climatic modifications that have a direct influence on vegetation phenology. Mountainous areas have a fragile biological environment, and vegetation phenology is relatively sensitive to urbanization. However, there is a paucity of research on the impact of urbanization in mountainous areas on vegetation phenology. The Urban Agglomeration of Central Yunnan (UACY) is located on the Yunnan–Guizhou Plateau in China. This study explored the vegetation phenological changes in different terrains from 2001 to 2020 in the UACY based on remote sensing data. Using the dynamic gradient method, we examined the response of vegetation phenology to urbanization from three aspects: urban–rural gradient, urbanization intensity (UI), and population density. The results showed that landform has a significant impact on SOS (start of growing season), with a topographic relief difference of 200 m acting as the dividing line. The findings derived from the dynamic gradient analysis indicate that UI has the most significant effect. SOS advances by 5.77 days (R2 = 0.96), EOS (end of growing season) advances by 2.30 days (R2 = 0.83), and LOS (length of growing season) lengthens by 2.59 days (R2 = 0.87) for every 10% increase in UI. This study has the potential to serve as a valuable resource for future urban planning and administration in the UACY. Additionally, it could provide decision-making support for the development of mountainous urban agglomerations in ecological environments.
The Effects of Music-Based Interventions for Pain and Anxiety Management during Vaginal Labour and Caesarean Delivery: A Systematic Review and Narrative Synthesis of Randomised Controlled Trials
Authors: Hunter Amy Rose , Heiderscheit Annie , Galbally Megan , Gravina Davide , Mutwalli Hiba , Himmerich Hubertus
Published: November 2023

Music-based interventions are not physically invasive, they usually have minimal side effects, and they are increasingly being implemented during the birthing process for pain and anxiety relief. The aim of this systematic review is to summarise and evaluate published, randomised controlled trials (RCTs) assessing the effects of music-based interventions for pain and anxiety management during vaginal labour and caesarean delivery. Following the PRISMA guidelines, a systematic search of the literature was conducted using: PsychInfo (Ovid), PubMed, and Web of Science. Studies were included in the review if they were RCTs that assessed the effects of music on pain and anxiety during vaginal and caesarean delivery by human mothers. A narrative synthesis was conducted on 28 identified studies with a total of 2835 participants. Most, but not all, of the included studies assessing music-based interventions resulted in reduced anxiety and pain during vaginal and caesarean delivery. Music as part of a comprehensive treatment strategy, participant-selected music, music coupled with another therapy, and relaxing/instrumental music was specifically useful for reducing light to moderate pain and anxiety. Music-based interventions show promising effects in mitigating pain and anxiety in women during labour. However, the long-term effects of these interventions are unclear.
Cellular Mechanisms of Singlet Oxygen in Photodynamic Therapy
Authors: Przygoda Maria , Bartusik-Aebisher Dorota , Dynarowicz Klaudia , Cieślar Grzegorz , Kawczyk-Krupka Aleksandra , Aebisher David
Published: November 2023

In this review, we delve into the realm of photodynamic therapy (PDT), an established method for combating cancer. The foundation of PDT lies in the activation of a photosensitizing agent using specific wavelengths of light, resulting in the generation of reactive oxygen species (ROS), notably singlet oxygen (1O2). We explore PDT’s intricacies, emphasizing its precise targeting of cancer cells while sparing healthy tissue. We examine the pivotal role of singlet oxygen in initiating apoptosis and other cell death pathways, highlighting its potential for minimally invasive cancer treatment. Additionally, we delve into the complex interplay of cellular components, including catalase and NOX1, in defending cancer cells against PDT-induced oxidative and nitrative stress. We unveil an intriguing auto-amplifying mechanism involving secondary singlet oxygen production and catalase inactivation, offering promising avenues for enhancing PDT’s effectiveness. In conclusion, our review unravels PDT’s inner workings and underscores the importance of selective illumination and photosensitizer properties for achieving precision in cancer therapy. The exploration of cellular responses and interactions reveals opportunities for refining and optimizing PDT, which holds significant potential in the ongoing fight against cancer.
Energy Regulation in Inflammatory Sarcopenia by the Purinergic System
Authors: Marco-Bonilla Miguel , Fresnadillo Maria , Largo Raquel , Herrero-Beaumont Gabriel , Mediero Aránzazu
Published: November 2023

The purinergic system has a dual role: the maintenance of energy balance and signaling within cells. Adenosine and adenosine triphosphate (ATP) are essential for maintaining these functions. Sarcopenia is characterized by alterations in the control of energy and signaling in favor of catabolic pathways. This review details the association between the purinergic system and muscle and adipose tissue homeostasis, discussing recent findings in the involvement of purinergic receptors in muscle wasting and advances in the use of the purinergic system as a novel therapeutic target in the management of sarcopenia.
Molecular Mechanisms Associated with Aging Kidneys and Future Perspectives
Authors: Jo Min-Jee , Lee Joo-Kyung , Kim Ji-Eun , Ko Gang-Jee
Published: November 2023

The rapid growth of the elderly population is making the need for extensive and advanced information about age-related organ dysfunction a crucial research area. The kidney is one of the organs most affected by aging. Aged kidneys undergo functional decline, characterized by a reduction in kidney size, decreased glomerular filtration rate, alterations in renal blood flow, and increased inflammation and fibrosis. This review offers a foundation for understanding the functional and molecular mechanisms of aging kidneys and for selecting identifying appropriate targets for future treatments of age-related kidney issues.
Proteomics and Metabolomics in Biomedicine
Authors: Santorelli Lucia , Caterino Marianna , Costanzo Michele
Published: November 2023

The technological advances of recent years have significantly enhanced medical discoveries [...]
Steroidal Alkaloids from Food Waste of Tomato Processing Inhibit Neuroblastoma Cell Viability
Authors: Santonocito Debora , Delli Carri Matteo , Campisi Agatina , Sposito Giovanni , Pellitteri Rosalia , Raciti Giuseppina , Cardullo Nunzio , Aquino Giovanna , Basilicata Manuela Giovanna , Pepe Giacomo , Pignatello Rosario , Puglia Carmelo
Published: November 2023

Nowadays, there is considerable attention toward the use of food waste from food processing as possible sources of compounds with health properties, such as anticancer activity. An example is tomato processing, which is responsible for generating a remarkable amount of waste (leaves, peel, seeds). Therefore, our goal was to evaluate the potential anticancer property of tomato extracts, in particular “Datterino” tomato (DT) and “Piccadilly” tomato (PT), and to study their phytochemical composition. Liquid chromatography with tandem mass spectrometry (LC/MS-MS) results showed that these extracts are rich in alkaloids, flavonoids, fatty acids, lipids, and terpenes. Furthermore, their potential anticancer activity was evaluated in vitro by MTT assay. In particular, the percentage of cell viability was assessed in olfactory ensheathing cells (OECs), a particular glial cell type of the olfactory system, and in SH-SY5Y, a neuroblastoma cell line. All extracts (aqueous and ethanolic) did not lead to any significant change in the percentage of cell viability on OECs when compared with the control. Instead, in SH-SY5Y we observed a significant decrease in the percentage of cell viability, confirming their potential anticancer activity; this was more evident for the ethanolic extracts. In conclusion, tomato leaves extracts could be regarded as a valuable source of bioactive compounds, suitable for various applications in the food, nutraceutical, and pharmaceutical fields.
Farnesoid X Receptor Agonist GW4064 Protects Lipopolysaccharide-Induced Intestinal Epithelial Barrier Function and Colorectal Tumorigenesis Signaling through the αKlotho/βKlotho/FGFs Pathways in Mice
Authors: Liu Hsuan-Miao , Chang Zi-Yu , Yang Ching-Wei , Chang Hen-Hong , Lee Tzung-Yan
Published: November 2023

The farnesoid X receptor (FXR)/βKlotho/fibroblast growth factors (FGFs) pathway is crucial for maintaining the intestinal barrier and preventing colorectal cancer (CRC). We used an FXR agonist, GW4064, and FXR-knockout (FXR-KO) mice to investigate the role of FXR/Klothos/FGFs pathways in lipopolysaccharide (LPS)-induced intestinal barrier dysfunction and colon carcinogenesis. The results showed that upregulation of FXR in enterocytes effectively ameliorated intestinal tight-junction markers (claudin1 and zonula occludens-1), inflammation, and bile acid levels, thereby protecting mice from intestinal barrier dysfunction and colon carcinogenesis. GW4064 treatment increased FXR, αKlotho, βKlotho, FGF19, FGF21, and FGF23 in wild-type mice exposed to LPS, while FXR-KO mice had decreased levels. FXR-KO mice exhibited elevated colon cancer markers (β-catenin, LGR5, CD44, CD34, and cyclin D1) under LPS, underscoring the pivotal role of FXR in inhibiting the development of colon tumorigenesis. The varying gut microbiota responses in FXR-KO mice versus wild-type mice post LPS exposure emphasize the pivotal role of FXR in preserving intestinal microbial health, involving Bacteroides thetaiotaomicron, Bacteroides acidifaciens, and Helicobacter hepaticus. Our study validates the effectiveness of GW4064 in alleviating LPS-induced disruptions to the intestinal barrier and colon carcinogenesis, emphasizing the importance of the FXR/αKlotho/βKlotho/FGFs pathway and the interplay between bile acids and gut microbiota.
Transcriptional Targeting of Dendritic Cells Using an Optimized Human Fascin1 Gene Promoter
Authors: Zeyn Yanira , Hobernik Dominika , Wilk Ulrich , Pöhmerer Jana , Hieber Christoph , Medina-Montano Carolina , Röhrig Nadine , Strähle Caroline F. , Thoma-Kress Andrea K. , Wagner Ernst , Bros Matthias , Berger Simone
Published: November 2023

Deeper knowledge about the role of the tumor microenvironment (TME) in cancer development and progression has resulted in new strategies such as gene-based cancer immunotherapy. Whereas some approaches focus on the expression of tumoricidal genes within the TME, DNA-based vaccines are intended to be expressed in antigen-presenting cells (e.g., dendritic cells, DCs) in secondary lymphoid organs, which in turn induce anti-tumor T cell responses. Besides effective delivery systems and the requirement of appropriate adjuvants, DNA vaccines themselves need to be optimized regarding efficacy and selectivity. In this work, the concept of DC-focused transcriptional targeting was tested by applying a plasmid encoding for the luciferase reporter gene under the control of a derivative of the human fascin1 gene promoter (pFscnLuc), comprising the proximal core promoter fused to the normally more distantly located DC enhancer region. DC-focused activity of this reporter construct was confirmed in cell culture in comparison to a standard reporter vector encoding for luciferase under the control of the strong ubiquitously active cytomegalovirus promoter and enhancer (pCMVLuc). Both plasmids were also compared upon intravenous administration in mice. The organ- and cell type-specific expression profile of pFscnLuc versus pCMVLuc demonstrated favorable activity especially in the spleen as a central immune organ and within the spleen in DCs.
Lost in Space? Unmasking the T Cell Reaction to Simulated Space Stressors
Authors: Miranda Silvana , Vermeesen Randy , Radstake Wilhelmina E. , Parisi Alessio , Ivanova Anna , Baatout Sarah , Tabury Kevin , Baselet Bjorn
Published: November 2023

The space environment will expose astronauts to stressors like ionizing radiation, altered gravity fields and elevated cortisol levels, which pose a health risk. Understanding how the interplay between these stressors changes T cells’ response is important to better characterize space-related immune dysfunction. We have exposed stimulated Jurkat cells to simulated space stressors (1 Gy, carbon ions/1 Gy photons, 1 µM hydrocortisone (HC), Mars, moon, and microgravity) in a single or combined manner. Pro-inflammatory cytokine IL-2 was measured in the supernatant of Jurkat cells and at the mRNA level. Results show that alone, HC, Mars gravity and microgravity significantly decrease IL-2 presence in the supernatant. 1 Gy carbon ion irradiation showed a smaller impact on IL-2 levels than photon irradiation. Combining exposure to different simulated space stressors seems to have less immunosuppressive effects. Gene expression was less impacted at the time-point collected. These findings showcase a complex T cell response to different conditions and suggest the importance of elevated cortisol levels in the context of space flight, also highlighting the need to use simulated partial gravity technologies to better understand the immune system’s response to the space environment.
Prostaglandin E2 Exposure Disrupts E-Cadherin/Caveolin-1-Mediated Tumor Suppression to Favor Caveolin-1-Enhanced Migration, Invasion, and Metastasis in Melanoma Models
Authors: Lobos-González Lorena , Oróstica Lorena , Díaz-Valdivia Natalia , Rojas-Celis Victoria , Campos America , Duran-Jara Eduardo , Farfán Nicole , Leyton Lisette , Quest Andrew F. G.
Published: November 2023

Caveolin-1 (CAV1) is a membrane-bound protein that suppresses tumor development yet also promotes metastasis. E-cadherin is important in CAV1-dependent tumor suppression and prevents CAV1-enhanced lung metastasis. Here, we used murine B16F10 and human A375 melanoma cells with low levels of endogenous CAV1 and E-cadherin to unravel how co-expression of E-cadherin modulates CAV1 function in vitro and in vivo in WT C57BL/6 or Rag−/− immunodeficient mice and how a pro-inflammatory environment generated by treating cells with prostaglandin E2 (PGE2) alters CAV1 function in the presence of E-cadherin. CAV1 expression augmented migration, invasion, and metastasis of melanoma cells, and these effects were abolished via transient co-expression of E-cadherin. Importantly, exposure of cells to PGE2 reverted the effects of E-cadherin expression and increased CAV1 phosphorylation on tyrosine-14 and metastasis. Moreover, PGE2 administration blocked the ability of the CAV1/E-cadherin complex to prevent tumor formation. Therefore, our results support the notion that PGE2 can override the tumor suppressor potential of the E-cadherin/CAV1 complex and that CAV1 released from the complex is phosphorylated on tyrosine-14 and promotes migration/invasion/metastasis. These observations provide direct evidence showing how a pro-inflammatory environment caused here via PGE2 administration can convert a potent tumor suppressor complex into a promoter of malignant cell behavior.
Post-Operative Thoracic Epidural Analgesia and Incidence of Major Complications according to Specific Safety Standardized Documentation: A Large Retrospective Dual Center Experience
Authors: Sarridou Despoina G. , Mouratoglou Sophia Anastasia , Mitchell Jeremy B. , Cox Felicia , Boutou Afroditi , Braoudaki Maria , Lambrou George I. , Konstantinidou Maria , Argiriadou Helena , Walker Christopher P. R.
Published: November 2023

(1) Background: Thoracic epidural analgesia is considered the gold standard in post-operative pain management following thoracic surgery. This study was designed to explore the safety of thoracic epidural analgesia and to quantify the incidence of its post-operative complications and side effects in patients undergoing thoracotomy for major surgery, such as resection of lung malignancies and lung transplantation. (2) Methods: This is a retrospective, dual-center observational study including patients that underwent major thoracic surgery including lung transplantation and received concurrent placement of thoracic epidural catheters for post-operative analgesia. An electronic system of referral and documentation of complications was used, and information was retrieved from our electronic critical care charting system. (3) Results: In total, 1145 patients were included in the study. None of the patients suffered any major complication, including hematoma, abscess, or permanent nerve damage. (4) Conclusions: the present study showed that in experienced centers, post-operative epidural analgesia in patients with thoracotomy is a safe technique, manifesting minimal, none-serious complications.
Geopolymers: Advanced Materials in Medicine, Energy, Anticorrosion and Environmental Protection
Authors: Kudłacik-Kramarczyk Sonia , Drabczyk Anna , Figiela Beata , Korniejenko Kinga
Published: November 2023

The initial predictions of the importance of geopolymers primarily assumed use mainly in the construction sector. However, as research progresses, it is becoming clear that these versatile materials demonstrate the ability to greatly exceed their original applications, as characterized in detail in this review article. To the best of our knowledge, there is no literature review concerning geopolymer materials that compiles the diverse applications of these versatile materials. This paper focuses on geopolymer applications beyond the construction industry. The surprising application potential of geopolymers in medicine has become a topic of particular interest. Therefore, considerable attention in this paper is devoted to characterizing the utility of these materials in tissue engineering, dentistry and drug delivery systems. Geopolymers not only have exceptional heat resistance and compressive strength, making them durable and resistant to manipulation (over five times less drug released from the geopolymer carrier compared to the commercial formulation), but also provide a robust solution for extended-release drug delivery systems, especially in opioid formulations. Their chemical stability, porous structure and ability to maintain structure after repeated regeneration processes speak to their potential in water treatment. Geopolymers, which excel in the energy industry as refractory materials due to their resistance to high temperatures and refractory properties, also present potential in thermal insulation and energy storage. It was demonstrated that geopolymer-based systems may even be 35% cheaper than conventional ones and show 70% lower thermal conductivity. In terms of protection against microorganisms, the possibility of modifying geopolymers with antimicrobial additives shows their adaptability, maintaining their effectiveness even under high-temperature conditions. Research into their use as anticorrosion materials is targeting corrosion-resistant coatings, with geopolymers containing graphene oxide showing particularly promising results. The multitude of potential applications for geopolymers in a variety of fields reflects their enormous potential. As research progresses, the scope of their possibilities continues to expand, offering innovative solutions to pressing global challenges.
An Equivalent Structural Stress-Based Frequency-Domain Fatigue Assessment Approach for Welded Structures under Random Loading
Authors: Kalu Uchenna , Liang Xihui
Published: November 2023

Welded structures under random loadings are usually susceptible to fatigue-induced failures that lead to significant economic and safety effects. However, accurately predicting these structures’ fatigue damage and life in the frequency domain remains challenging due to the limitations associated with using traditional weld stress extrapolation methods, such as nominal, hotspot, and notch stress methods. These methods struggle with precisely defining and characterizing the stresses at the weld toe and root as they vary depending on factors like weld stress concentration effects, joint geometry, and loading modes. This research introduces an Equilibrium Equivalent Structural Stress (EESS)-based frequency-domain fatigue analysis approach for welded structures subjected to random loading. The proposed method utilizes the EESS formulations, which are based on the decomposition and characterization of weld toe stresses with a single stress parameter, together with incorporating structural dynamic properties’ effects on the stresses acting on the weld joints and the corresponding accumulated fatigue damage of the structure. The numerical demonstration and validation of the proposed method have been performed using a welded Rectangular Hollow Section (RHS) T-joint structure subjected to stationary random fatigue loading. The proposed method’s fatigue damage and life results are compared with the fatigue test data and the equivalent hotspot stress extrapolation-based technique results.
Fabrication of Al-Ni Alloys for Fast Hydrogen Production from Hydrolysis in Alkaline Water
Authors: Kwon JaeYoung , Eom KwangSup , Kim MinJoong , Toor Ihsan , Oh SeKwon , Kwon HyukSang
Published: November 2023

Hydrogen generation through the hydrolysis of aluminum alloys has attracted significant attention because it generates hydrogen directly from alkaline water without the need for hydrogen storage technology. The hydrogen generation rate from the hydrolysis of aluminum in alkaline water is linearly proportional to its corrosion rate. To accelerate the corrosion rate of the aluminum alloy, we designed Al-Ni alloys by continuously precipitating an electrochemically noble Al3Ni phase along the grain boundaries. The Al-0.5~1 wt.% Ni alloys showed an excellent hydrogen generation rate of 16.6 mL/cm2·min, which is about 6.4 times faster than that of pure Al (2.58 mL/cm2·min). This excellent performance was achieved through the synergistic effects of galvanic and intergranular corrosion on the hydrolysis of Al. By raising the solution temperature to 50 °C, the optimal rate of hydrogen generation of Al-1 wt.% Ni in 10 wt.% NaOH solutions at 30 °C can be further increased to 54.5 mL/cm2·min.
Hot Deformation Behavior and Mechanisms of SiC Particle Reinforced Al-Zn-Mg-Cu Alloy Matrix Composites
Authors: Diao Enze , Fan Jianzhong , Yang Zhiyu , Lv Zhaochong , Gao Hao , Nie Junhui
Published: November 2023

A systematic and comprehensive analysis of the hot deformation and mechanisms of SiC particle-reinforced aluminum matrix composites is significant for optimizing the processing of the composites and obtaining the desired components. Based on this, related research on 11 vol% SiCp particle-reinforced 7050Al matrix composites was carried out. Hot compression experiments were carried out on the Gleeble-3500 thermal simulator to study the hot deformation behavior of composites at the temperature of 370–520 °C and strain rate of 0.001–10 s−1. The hyperbolic sine constitutive equation of the material was established, and the processing map was calculated. Combining the typical metallograph and misorientation angle distribution, the microstructure evolution mechanism of composites was analyzed, and the effect of particles on recrystallization behavior was investigated. Under certain process conditions, the dominant deformation mechanism of composites changed from dynamic recovery (DRV) to dynamic recrystallization (DRX), and the grain boundary sliding mechanism began to play a role. In addition, high temperature tensile and elongation at break were tested, and it was found that the dominant form of fracture failure changed from brittle fracture of the particles to ductile fracture of the matrix as the temperature increased.
Experimental Study on Tunnel Bottom Deformation Trend in Gently Inclined Layered Shale Based on the Energy Index
Authors: Chen Binke , Tan Yinjun , Deng Yuan , Liu Zheng , Meng Wei
Published: November 2023

Influenced by the anisotropy and water-softening characteristics of gently inclined layered shale, many tunnels have encountered bottom deformation issues during construction and operation, which severely impact the safety of tunnel structures. The energy evolution law during rock deformation and damage can provide support for the assessment and prediction of structure deformation. However, most studies have been conducted on enstatite, granite, and sandstone with limited research on shale. In this study, both conventional and single-cyclic loading-and-unloading uniaxial compression tests were conducted on shale specimens with varying dip angles of the structural plane (Dφ) and water content (Wc) in addressing the most typical layered shale in the Chaoyang Tunnel. The energy evolution features of rock samples at each stage of the tests were analyzed to determine the discriminating indicator (SC) for tunnel bottom deformation tendency. The indicator was based on the elastic strain energy (Uei) and the post-peak dissipation energy (Udi). The results demonstrated that the Dφ and Wc directly affected the energy storage and dissipation process of rock specimens, which in turn enabled them to exhibit different damage evolution features. The Uei and the total input energy (Uli) satisfied a linear relationship, which was determined by the Dφ and Wc of rock specimens. The energy evolution-based indicator SC can accurately characterize the bottom deformation of the tunnel constructed in a gently inclined layered shale stratum. The findings can offer a scientific foundation for rational evaluation of the structure deformation of tunnels under construction.
Poly(ethylene glycol)-Engrafted Graphene Oxide for Gene Delivery and Nucleic Acid Amplification
Authors: Chauhan Khushbu , Woo Jin , Jung Woong , Kim Dong-Eun
Published: November 2023

Graphene oxide (GO) is an oxidized form of graphene accommodating various oxygen-containing functional groups such as hydroxyl, epoxy, and carboxyl groups on its surface. GO has been extensively utilized in various biomedical applications including the delivery of biomolecules and the development of biosensors owing to its beneficial properties such as high surface area, nucleic acid adsorption, and fluorescence quenching through fluorescence resonance energy transfer (FRET). However, despite these favorable properties, the direct utilization of GO in these applications is often limited by low dispersibility in a physiological medium, cytotoxicity, low biocompatibility, and a strong binding affinity of nucleic acids to GO surface. The large surface area of GO and the presence of various functional groups on its surface make it highly amenable to facile surface modifications, offering scope for GO surface functionalization to overcome these limitations. When polyethylene glycol (PEG), which is a biocompatible polymer, is conjugated to GO, the PEGylated GO enhances the biocompatibility and dispersibility, reduces cytotoxicity, and allows controlled drug delivery with controllable binding affinity towards nucleic acid. PEG-engrafted GO retains the beneficial properties of GO while effectively addressing its limitations, rendering it suitable for various biomedical applications. In this review, we present the recent advancements of PEGylated GO in gene/drug delivery and the facilitation of nucleic acid amplification techniques, which aid in the development of therapeutic and diagnostic tools, respectively.
Testing the Influence of Metakaolinite and Zeolite on the Adhesion of BFRP and GFRP Bars to Concrete
Authors: Krassowska Julita , Wolka Paweł , Protchenko Kostiantyn , Vidales-Barriguete Alejandra
Published: November 2023

Today’s sustainable development policy in Europe, which is driven by concerns about the greenhouse effect and environmental protection, mandates a reduction in CO2 emissions into the atmosphere. The cement industry and steel mills that produce reinforcing bars are among the largest and most emissions-intensive sectors emitting CO2 into the atmosphere. This article analyzes the possibility of achieving significant reductions in CO2 emissions by using basalt bars (BFRP) and glass bars (GFRP) in concrete structures, and—in the case of concrete—by using cement with the addition of metakaolinite and zeolite. There is a lack of literature reports on whether modifying concrete with the additions of metakaolinite and zeolite as substitutes for part of the cement affects the adhesion of FRP bars to concrete. It can be assumed, however, that improving the microstructure of concrete also improves the contact zone between the bar and the concrete. The aim of this research is to fill the aforementioned gap in the literature data by determining how the presence of metakaolinite and zeolite affects the adhesion of reinforcing bars to concrete and testing selected properties of hardened concrete. The test samples were prepared following the appropriate beam test procedure. The obtained results made it possible to perform a comparative analysis of reference samples and those with metakaolinite and zeolite additions. The research showed that introducing active pozzolanic additives in the form of metakaolinite and zeolite into concrete improved adhesion stress values by approximately 20% for glass GFRP bars and 15% for basalt BFRP bars, especially in the destruction phase.
Resistomycin Inhibits Wnt/β-Catenin Signaling to Induce the Apoptotic Death of Human Colorectal Cancer Cells
Authors: Zhu Yaoyao , Zhang E , Gao Huan , Shang Chuangeng , Yin Mengxiong , Ma Mingtao , Liu Yu , Zhang Xuanfeng , Li Xia
Published: November 2023

Resistomycin is a natural antibiotic related to quinone that has been shown to exhibit robust antitumor activity. To further characterize the mechanistic basis for such activity, human colorectal cancer (CRC) cells were selected as a model to explore the role of Wnt/β-catenin signaling in the ability of resistomycin to induce apoptotic cell death. These analyses revealed that resistomycin was able to suppress β-catenin, TCF4, and GSK-3β expression, together with that of the downstream targets c-Myc and survivin. This coincided with elevated cleaved caspase-3 and Bax protein levels and a decline in Bcl-2 content. When β-catenin was silenced, this further enhanced the ability of resistomycin to induce apoptotic CRC cell death, whereas this apoptotic process was partially ablated when cells were treated using lithium chloride to activate Wnt/β-catenin signaling. Overall, these results support a model wherein resistomycin inhibits Wnt/β-catenin signaling within CRC cells, thereby inducing apoptotic death. Further research may be warranted to better clarify the potential utility of this compound as a candidate drug for use in the treatment of patients suffering from this form of cancer.
Corrosion of Fixed Orthodontic Appliances: Causes, Concerns, and Mitigation Strategies
Authors: Fróis António , Santos Ana Cristina , Louro Cristina Santos
Published: November 2023

The orthodontic supply market is a prosperous billion-dollar industry, driven by an increasing demand for orthodontic appliances. The supremacy of metallic first-generation biomaterials is evident for manufacturing brackets, archwires, bands, and other components due to their well-recognized chemical inertness, spontaneous passivation, biocompatibility, and favorable mechanical properties combination. However, the oral cavity is the ultimate corrosion-promoting environment for any metallic material. In this work, the general picture of the intraoral degradation of fixed orthodontic appliances is first addressed, from the causes to the harmful effects and their oral clinical implications. Current mitigation strategies are also pointed out, including the alloys’ bulk composition adjustment combined with new and advanced manufacturing processes and/or their surface treatment or coating deposition. The versatile use of thin films and coatings stands out with different deposition technologies: Many in vivo and in vitro efforts have been devoted to oral aging, from monolithic to composite architectures and micro- to nano-scale materials, to meet the best and safest oral practice demands. Unfortunately, literature data suggest that even the existing commercially available protective coatings have drawbacks and are fallible. Further multidisciplinary research is still required to effectively mitigate the corrosion behavior of fixed orthodontic appliances.
Uranium in Fluorite, a Case Study: The La Azul Fluorspar Deposit, Taxco, Guerrero, Mexico
Authors: Pi-Puig Teresa , Solé Jesús , Alba-Aldave Leticia
Published: November 2023

Uranium can be found in the Earth’s crust in different reservoirs, with igneous rocks being the primary source of this element from which many types of secondary deposits are formed. Fluorspar deposits generally do not contain uranium, but in some cases, fluorite can carry both uranium in solid solutions and inclusions of uranium minerals. We studied the concentration (ICP-MS), composition (electronic microprobe), and spatial distribution (microscopy and auto-radiography) of elemental uranium and uranium minerals at different scales (microscopy and auto-radiography in fluorite from the La Azul fluorspar deposit (Taxco, Mexico) to assess the origin of uranium and its significance in this ore deposit. Auto-radiography images with the CR-39 detector were found to be impressive in their ability to elucidate uranium distribution at the millimeter scale. The limit between the solid solution of elemental uranium in natural fluorite and the appearance of uranium oxides as inclusions appeared to be between 20 μg g−1 and 40 μg g−1 bulk uranium concentration in this fluorspar ore. The maximum concentration of U in fluorite from the La Azul deposit was about 100 μg g−1. Using Raman spectroscopy and microprobe analysis, we identified the micro-inclusions of uranium minerals as uraninite (of the pitchblende variety); its composition suggested a hydrothermal origin for this fluorspar deposit. We also calculated a chemical age that can be compared with the previously published regional geology and isotopic (U-Th-Sm)/He ages in fluorite. Micro-thermometric studies of fluid inclusions were carried out in different samples of uranium-rich fluorite to identify the nature and origin of the mineralizing fluid and the precipitation mechanisms of uranium minerals. We concluded that the uranium-rich fluorite precipitated in the initial phases of mineralization from a reducing fluid, with low salinity (<8% NaCl eq.) and an intermediate temperature (110–230 °C), and that the presence of organic compounds and sulfides (mainly pyrite) favored the simultaneous precipitation of uraninite (pitchblende variety) and fluorite.
Calcium Carbonate Precipitation Behavior in the System Ca-Me2+-CO3-H2O (Me2+ = Co, Ni, Cu, Fe): Ion Incorporation, Effect of Temperature and Aging
Authors: Vereshchagin Oleg S. , Chernyshova Irina A. , Kuz’mina Maria A. , Frank-Kamenetskaya Olga V.
Published: November 2023

Crystalline calcium carbonates (CCCs) are among the most widespread minerals on the Earth’s surface and play a crucial role in the global carbon cycle, heavy metal sorption and incorporation. Among the numerous factors that influence the precipitation of CCCs from solution, the most determinant are the presence of additives in the mineral-forming medium, temperature, and crystallization time (aging time). The current work fills the gaps in the study of calcium carbonate crystallization from heavy metal (Me2+ = Co, Ni, Cu Fe)-containing solutions (Me2+/Ca 0.005–1.600) at different temperatures (3 and 23 °C) and aging times (21–158 days). The resulting precipitates were studied using optical and scanning electron microscopy, powder X-ray diffraction and energy-dispersive X-ray spectroscopy. Three crystalline calcium carbonates (synthetic analogues of calcite, aragonite and monohydrocalcite), as well as amorphous carbonate (AC), were found in the resulting precipitates. Temperature and aging time have a considerable effect on the phase composition, morphology and heavy metal content in CCCs. Low temperature (3 °C) and short aging times are generally favorable for the formation of monohydrocalcite and amorphous carbonate, while calcite tends to form at a higher temperature (23 °C) and in long-term experiments. Heavy metals can be incorporated into the calcite/monohydrocalcite crystal lattice in sufficient amounts, while aragonite can host a very small amount of Me2+ (or none). Calcite can concentrate Co (up to ~0.25 atoms per formula unit (apfu)) and Ni/Cu (up to ~0.05 apfu), while its Fe content is very close to the detection limits. Calcite precipitated at a higher Me2+/Ca ratio and temperature (23 °C) contains less Me2+ compared to calcite precipitated at a lower Me2+/Ca ratio and temperature (3 °C). Monohydrocalcite can host up to ~0.1 apfu of Co/Ni/Cu with no detectable preference for Me2+. The amount of Me2+ in monohydrocalcite decreases as aging time or temperature increases. It is worth noting that AC is the main carrier of heavy metals in the system being investigated and it should be considered the main host phase in heavy metal adsorption from aqueous solutions. The results obtained can be used to solve environmental issues and in mineral resource management.
Deep Structure of Nanling-Xuancheng Ore District, Eastern China: Insights from Integrated Geophysical Exploration
Authors: Guo Dong , Lan Xueyi , Lu Sanming , Zhang Yuanyuan , Ding Wenxiang , You Miao , Zhang Kun , Zhao Lili , Wang Yunyun , Zhang Shasha , Zhang Huijie , Tao Long
Published: November 2023

As the depth of mineral exploration increases, integrated geophysical methods are increasingly playing a crucial role in prospecting deep structures at the district scale. The Nanling-Xuancheng ore district is the eighth ore district in the middle-lower Yangtze metallogenic belt in China. To reveal the deep structure of the mining district, this study mainly focuses on regional high-precision gravity and magnetic data and integrates the interpretation of magnetotelluric and reflection seismic data from a key area. By using a 2.5D joint inversion method with prior information constraints, new insights into the deep structures, tectonic deformation, and magmatic activity are obtained. Structurally, the Nanling-Xuancheng ore district presents a structural pattern of “two uplifts and two depressions” composed of multi-level thrust-overturned and folds formed by Mesozoic depressions, which has a three-layer structure in the vertical direction (shallower than 10 km). Tectonically, the main faults in the study area trend NW, which intersect with NE-trending and EW-trending faults to form a branching structure from deep to shallow. The fault intersections provide pathways for magma intrusion. The distribution of deep-seated concealed magmatic rocks shows the characteristic pattern of “a primary magma source spawning multiple subsidiary intrusion”.
Cytotoxicity of PEG-Coated Gold and Gold–Iron Alloy Nanoparticles: ROS or Ferroptosis?
Authors: de Faria Clara M. G. , Bissoli Michael , Vago Riccardo , Spinelli Antonello E. , Amendola Vincenzo
Published: November 2023

Nanomedicine relies on the exploitation of nanoscale constructs for therapeutic and diagnostic functions. Gold and gold–iron alloy nanoparticles (NPs) are two examples of nanomaterials with favorable features for use in nanomedicine. While gold NPs have been studied extensively in the last decades, they are not biodegradable. Nonetheless, biodegradation was recently observed in gold alloys with iron obtained using laser ablation in liquid (LAL). Hence, there is a significant interest in the study of the biological effects of gold and gold–iron alloy nanoparticles, starting from their tolerability and cytotoxicity. In this study, these two classes of NPs, obtained via LAL and coated with biocompatible polymers such as polyethylene glycol, were investigated in terms of their cytotoxicity in fibroblasts, prostate cancer cells (PC3) and embryonic kidney cells (HEK). We also explored the effects of different synthetic procedures, stabilizing additives, and the possible mechanisms behind cell mortality such as the formation of reactive oxygen species (ROS) or ferroptosis. NPs larger than 200 nm were associated with lower cell tolerability. The most tolerable formulations were pure PEG-Au NPs, followed by PEG-Au–Fe NPs with a hydrodynamic size < 50 nm, which displayed a toxicity of only 20% in fibroblasts after 72 h of incubation. In addition, tumor cells and highly proliferating HEK cells are more sensitive to the NPs than fibroblasts. However, a protective effect of catalase was found for cells incubated with PEG-Au–Fe NPs, indicating an important role of hydrogen peroxide in alloy NP interactions with cells. These results are crucial for directing future synthetic efforts for the realization of biocompatible Au NPs and biodegradable and cytocompatible Au–Fe alloy NPs. Moreover, the correlation of the cytocompatibility of NPs with ROS and ferroptosis in cells is of general interest and applicability to other types of nanomaterials.
Facile Synthesis of Ce-MOF for the Removal of Phosphate, Fluoride, and Arsenic
Authors: Zhang Lili , Mao Decheng , Qu Yining , Chen Xiaohong , Zhang Jindi , Huang Mengyang , Wang Jiaqiang
Published: November 2023

Ce-MOF was synthesized by a solvothermal synthesis method and was used to simultaneously remove phosphate, fluoride and arsenic (V) from water by adsorption. Ce-MOF was characterized by a nitrogen adsorption–desorption isotherm, scanning electron microscopy, and infrared spectroscopy. The effects of initial concentration, adsorption time, adsorption temperature, pH value and adsorbent on the adsorption properties were investigated. A Langmuir isotherm model was used to fit the adsorption data, and the adsorption capacity of phosphate, fluoride, and arsenic (V) was calculated to be 41.2 mg·g−1, 101.8 mg·g−1 and 33.3 mg·g−1, respectively. Compared with the existing commercially available CeO2 and other MOFs, Ce-MOF has a much higher adsorption capacity. Furthermore, after two reuses, the performance of the adsorbent was almost unchanged, indicating it is a stable adsorbent and has good application potential in the field of wastewater treatment.
The IRONy in Athletic Performance
Authors: Kardasis William , Naquin Ethan R. , Garg Richa , Arun Tejas , Gopianand Jyotsna S. , Karmakar Eshani , Gnana-Prakasam Jaya P.
Published: November 2023

Iron is an essential micronutrient for athletes, intricately linked to their performance, by regulating cellular respiration and metabolism. Impaired iron levels in the body can significantly hinder athletic performance. The increased demand for iron due to exercise, coupled with potential dietary iron insufficiencies, particularly among endurance athletes, amplifies the risk of iron deficiency. Moreover, prolonged exercise can impact iron absorption, utilization, storage, and overall iron concentrations in an athlete. On the contrary, iron overload may initially lead to enhanced performance; however, chronic excess iron intake or underlying genetic conditions can lead to detrimental health consequences and may negatively impact athletic performance. Excess iron induces oxidative damage, not only compromising muscle function and recovery, but also affecting various tissues and organs in the body. This narrative review delineates the complex relationship between exercise and iron metabolism, and its profound effects on athletic performance. The article also provides guidance on managing iron intake through dietary adjustments, oral iron supplementation for performance enhancement in cases of deficiency, and strategies for addressing iron overload in athletes. Current research is focused on augmenting iron absorption by standardizing the route of administration while minimizing side effects. Additionally, there is ongoing work to identify inhibitors and activators that affect iron absorption, aiming to optimize the body’s iron levels from dietary sources, supplements, and chelators. In summary, by refining the athletic diet, considering the timing and dosage of iron supplements for deficiency, and implementing chelation therapies for iron overload, we can effectively enhance athletic performance and overall well-being.
Protein Supplementation May Dampen Positive Effects of Exercise on Glucose Homeostasis: A Pilot Weight Loss Intervention
Authors: Batsis John A. , Batchek Dakota J. , Petersen Curtis L. , Gross Danae C. , Lynch David H. , Spangler Hillary B. , Cook Summer B.
Published: November 2023

Background: The role of protein in glucose homeostasis has demonstrated conflicting results. However, little research exists on its impact following weight loss. This study examined the impact of protein supplementation on glucose homeostasis in older adults >65 years with obesity seeking to lose weight. Methods: A 12-week, nonrandomized, parallel group intervention of protein (PG) and nonprotein (NPG) arms for 28 older rural adults (body mass index (BMI) ≥ 30 kg/m2) was conducted at a community aging center. Both groups received twice weekly physical therapist-led group strength training classes. The PG consumed a whey protein supplement three times per week, post-strength training. Primary outcomes included pre/post-fasting glucose, insulin, inflammatory markers, and homeostasis model assessment of insulin resistance (HOMA-IR). Results: Mean age and baseline BMI were 72.9 ± 4.4 years and 37.6 ± 6.9 kg/m2 in the PG and 73.0 ± 6.3 and 36.6 ± 5.5 kg/m2 in the NPG, respectively. Mean weight loss was −3.45 ± 2.86 kg in the PG and −5.79 ± 3.08 kg in the NPG (p < 0.001). There was a smaller decrease in pre- vs. post-fasting glucose levels (PG: −4 mg ± 13.9 vs. NPG: −12.2 ± 25.8 mg/dL; p = 0.10), insulin (−7.92 ± 28.08 vs. −46.7 ± 60.8 pmol/L; p = 0.01), and HOMA-IR (−0.18 ± 0.64 vs. −1.08 ± 1.50; p = 0.02) in the PG compared to the NPG. Conclusions: Protein supplementation during weight loss demonstrated a smaller decrease in insulin resistance compared to the NPG, suggesting protein may potentially mitigate beneficial effects of exercise on glucose homeostasis.
Effects of Dietary Methionine Restriction on Cognition in Mice
Authors: Lail Hannah , Mabb Angela M. , Parent Marise B. , Pinheiro Filipe , Wanders Desiree
Published: November 2023

Dietary restriction of the essential amino acid, methionine, has been shown to induce unique metabolic protection. The peripheral benefits of methionine restriction (MR) are well established and include improvements in metabolic, energy, inflammatory, and lifespan parameters in preclinical models. These benefits all occur despite MR increasing energy intake, making MR an attractive dietary intervention for the prevention or reversal of many metabolic and chronic conditions. New and emerging evidence suggests that MR also benefits the brain and promotes cognitive health. Despite widespread interest in MR over the past few decades, many findings are limited in scope, and gaps remain in our understanding of its comprehensive effects on the brain and cognition. This review details the current literature investigating the impact of MR on cognition in various mouse models, highlights some of the key mechanisms responsible for its cognitive benefits, and identifies gaps that should be addressed in MR research moving forward. Overall findings indicate that in animal models, MR is associated with protection against obesity-, age-, and Alzheimer’s disease-induced impairments in learning and memory that depend on different brain regions, including the prefrontal cortex, amygdala, and hippocampus. These benefits are likely mediated by increases in fibroblast growth factor 21, alterations in methionine metabolism pathways, reductions in neuroinflammation and central oxidative stress, and potentially alterations in the gut microbiome, mitochondrial function, and synaptic plasticity.