bluuebunny/crossref_metadata_2025 huggingface dataset:
Electrical Impedance Tomography Technical Contributions for Detection and 3D Geometric Localization of Breast Tumors: A Systematic Review
Authors: Gómez-Cortés Juan Carlos , Díaz-Carmona José Javier , Padilla-Medina José Alfredo , Calderon Alejandro Espinosa , Gutiérrez Alejandro Israel Barranco , Gutiérrez-López Marcos , Prado-Olivarez Juan
Published: March 2022

Impedance measuring acquisition systems focused on breast tumor detection, as well as image processing techniques for 3D imaging, are reviewed in this paper in order to define potential opportunity areas for future research. The description of reported works using electrical impedance tomography (EIT)-based techniques and methodologies for 3D bioimpedance imaging of breast tissues with tumors is presented. The review is based on searching and analyzing related works reported in the most important research databases and is structured according to the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) parameters and statements. Nineteen papers reporting breast tumor detection and location using EIT were systematically selected and analyzed in this review. Clinical trials in the experimental stage did not produce results in most of analyzed proposals (about 80%), wherein statistical criteria comparison was not possible, such as specificity, sensitivity and predictive values. A 3D representation of bioimpedance is a potential tool for medical applications in malignant breast tumors detection being capable to estimate an ap-proximate the tumor volume and geometric location, in contrast with a tumor area computing capacity, but not the tumor extension depth, in a 2D representation.
Wideband Doherty Power Amplifier: A Design Approach
Authors: Moreno Rubio Jorge Julián , Angarita Malaver Edison Ferney , Lara González Luis Ángel
Published: March 2022

This paper presents a simple method to design wideband Doherty power amplifiers (DPAs) based on the synthesis of a combiner network which can mimic the response of an ideal compensation of the device reactive output equivalent network and exploit the maximum power capabilities of the device. Using the Wolfspeed’s CGH40006 and CG2H40025 GaN HEMT devices, two DPAs were designed and simulated to demonstrate the effectiveness of the proposed approach. In both cases, a 1.4 GHz bandwidth was obtained together with an efficiency higher than 44 and 49% at 6 dB OBO. The saturated output power was higher than 41.2 and 47 dBm over the band, for the DPAs using the CGH40006 and CG2H40025 devices, respectively.
Quality Study and Numerical Simulation Analysis of Solid–Liquid Two-Phase Magnetic Fluid Polishing Seven-Order Variable-Diameter Pipe
Authors: Guo Jing , Gui Lin , Hou Wei , Sun Liwei , Liu Yang , Li Junye
Published: March 2022

Variable-diameter pipe parts have been widely used in the automobile industry, aerospace industry, and other fields. To enhance the surface quality of variable-diameter pipe and explore the effect of solid–liquid two-phase magnetic fluid (SLTPMF) on polishing, in this paper, the seven-order variable-diameter pipe with symmetrical structure is taken as the research object to carry out experimental research and numerical simulation. The experimental research shows that the best surface roughness is reduced by an order of magnitude to Ra 0.054 μm. The solid–liquid two-phase magnetic fluid polishing (SLTPMFP) technology has reliability and superiority in improving the roughness of variable-diameter pipe parts. The simulation results show that the wall shear stress of solid–liquid two-phase magnetic fluid on the wall surface of the workpiece affects the improvement of roughness. The greater the wall shear force, the better the surface roughness can be obtained. The velocity and dynamic pressure cloud diagram show that the velocity and dynamic pressure on the center axis of the workpiece first increase and then decrease with the flow of the magnetic fluid. The velocity and dynamic pressure on the near wall surface are reduced due to the shear collision with the workpiece. This work can provide technical and theoretical support for the actual production of SLTPMF precision polishing.
Effect of Non-Lethal Selection on Spontaneous Revertants of Frameshift Mutations: The Escherichia coli hisF Case
Authors: Del Duca Sara , Puglia Anna Maria , Calderone Vito , Bazzicalupo Marco , Fani Renato
Published: March 2022

Microorganisms possess the potential to adapt to fluctuations in environmental parameters, and their evolution is driven by the continuous generation of mutations. The reversion of auxotrophic mutations has been widely studied; however, little is known about the reversion of frameshift mutations resulting in amino acid auxotrophy and on the structure and functioning of the protein encoded by the revertant mutated gene. The aims of this work were to analyze the appearance of reverse mutations over time and under different selective pressures and to investigate revertant enzymes’ three-dimensional structures and their correlation with a different growth ability. Escherichia coli FB182 strain, carrying the hisF892 single nucleotide deletion resulting in histidine auxotrophy, was subjected to different selective pressures, and revertant mutants were isolated and characterized. The obtained results allowed us to identify different indels of different lengths located in different positions in the hisF gene, and relations with the incubation time and the selective pressure applied were observed. Moreover, the structure of the different mutant proteins was consistent with the respective revertant ability to grow in absence of histidine, highlighting a correlation between the mutations and the catalytic activity of the mutated HisF enzyme.
Estimation of the Turkish Boron Exportation to Europe
Authors: Elevli Birol , Yaman İrem , Laratte Bertrand
Published: March 2022

Borate is an essential material to numerous industries and even to individual countries’ economies, defense, and politics. Almost all industries need borates for production, and almost everybody needs their products. Borate is a compound that contains or supplies boric oxide (B2O3). Among the minerals that contain boric oxide, there are only four minerals significant from an economic standpoint, namely borax (tincal), colemanite, ulexite, and kernite. Turkey has almost 70% of all known reserves in the world. Therefore, borates and their products could be one of the main topics for sustainable development in the whole world. The recent development and pursuit of new boron-consuming technologies and alternative products to existing borate-consuming products introduce additional uncertainty to the sustainability of boron minerals. Therefore, the European Union (EU) Commission also declared borate one of the 30 critical raw materials. Turkey is a prosperous country in terms of boron reserves, and it exports almost 96% of borates’ production. In order to better understand the relation between borate minerals and borate products, a material flow analysis (MFA) study has been carried out within the content of this work in order to update the data about the current status of boron. For this purpose, a system has been established that shows the flow of boron material. The extraction, enrichment, and refining processes of boron products are drawn. The results indicate that about 41% of extracted colemanite ore is converted into refined borate, about 31% of tincal ore is converted to refined borate, and 4% of tincal ore is converted to end-usage products, such as detergent. The correctness of the data and the sensitivity of the processes are all estimated values. The results can help in the development of boron sustainability and boron production strategies. The MFA study on tincal and colemanite ore may be an example of boron studies in different countries.
Microwave-Assisted Synthesis, Biological Activity Evaluation, Molecular Docking, and ADMET Studies of Some Novel Pyrrolo [2,3-b] Pyrrole Derivatives
Authors: Kamel Moumen S. , Belal Amany , Aboelez Moustafa O. , Shokr E. Kh. , Abdel-Ghany H. , Mansour Hany S. , Shawky Ahmed M. , El-Remaily Mahmoud Abd El Aleem Ali Ali
Published: March 2022

Novel pyrrolo [2,3-b] pyrrole derivatives were synthesized and their hypolipidemic activity was assessed in hyperlipidemic rats. The chemical structures of the new derivatives were confirmed through spectral analysis. Compounds 5 and 6 were revealed to be the most effective hypolipidemic agents, with considerable hypocholesterolemic and hypotriglyceridemic effects. They appear to be promising candidates for creating new powerful derivatives with anti-atherosclerotic and hypolipidemic properties. As for antimicrobial activity, some of the tested compounds showed moderate activity against Pseudomonas aeruginosa: compound 2 revealed an MIC value of 50 μg/mL, compared to 25 μg/mL for ciprofloxacin. Compound 3 showed good antimicrobial activity against Staphylococcus aureus, comparable to ciprofloxacin, and roughly half the activity of ampicillin, according to MIC values. Compound 2 has an MIC approximately 25% of that of clotrimazole against Candida albicans. Compound 2 also showed the highest antioxidant activity with 59% inhibition of radical scavenging activity. Additionally, the cytotoxic activity of these new derivatives 1–7 was investigated and most of them showed good anticancer activity against the three tested cell lines.
Recent Advances in the Synthesis of Isoquinoline-Fused Benzimidazoles
Authors: Twardy Dylan J. , Wheeler Kraig A. , Török Béla , Dembinski Roman
Published: March 2022

This review includes recent developments in the synthesis of benzo[4,5]imidazo[2,1-a]isoquinolines with particular attention given to categorizing protocols based on the structural features of the ring architecture and crystallographically characterized reaction products.
Influence of Rootstock on Yield Quantity and Quality, Contents of Biologically Active Compounds and Antioxidant Activity in Regent Grapevine Fruit
Authors: Klimek Kamila , Kapłan Magdalena , Najda Agnieszka
Published: March 2022

The cultivation of vines in temperate climates poses many difficulties to be overcome. The soil and climatic conditions in Poland limit the choice of vine varieties that can be used in the field; therefore, growers are often limited to varieties that are tolerant to extreme winter temperatures and spring frosts and to cultivars that are able to achieve optimum berry maturity at the end of the season. The study evaluated the effect of six rootstock types and own-root bushes on yield quantity and quality and on the content of biologically active compounds and antioxidant activity in Regent grapevine fruit. The research was conducted in 2015 at NOBILIS Vineyard (50°39′ N; 21°34′ E) in the Sandomierz Upland. Among the evaluated rootstocks, 125AA turned out to exert the significantly best effect on the yield, grape and berry weight, and number of grapes per bush. The fruit from bushes grafted on the 5BB rootstock were characterised by the highest content of L-ascorbic acid and tannins.
Isolation and Characterization of Bacteriocin-Producing Lacticaseibacillus rhamnosus XN2 from Yak Yoghurt and Its Bacteriocin
Authors: Wei Yonghua , Wang Jinze , Liu Zhe , Pei Jinjin , Brennan Charles , Abd El-Aty A.M.
Published: March 2022

Lactic acid bacteria (LAB) produce antimicrobial substances that could potentially inhibit the growth of pathogenic and food spoilage microorganisms. Lacticaseibacillus rhamnosus XN2, isolated from yak yoghurt, demonstrated antibacterial activity against Bacillus subtilis, B. cereus, Micrococcus luteus, Brochothrix thermosphacta, Clostridium butyricum, S. aureus, Listeria innocua CICC 10416, L. monocytogenes, and Escherichia coli. The antibacterial activity was estimated to be 3200 AU/mL after 30 h cultivation. Time-kill kinetics curve showed that the semi-purified cell-free supernatants (CFS) of strain XN2 possessed bactericidal activity. Flow cytometry analysis indicated disruption of the sensitive bacteria membrane by semi-purified CFS, which ultimately caused cell death. Interestingly, sub-lethal concentrations of semi-purified CFS were observed to reduce the production of α-haemolysin and biofilm formation. We further investigated the changes in the transcriptional level of luxS gene, which encodes signal molecule synthase (Al-2) induced by semi-purified CFS from strain XN2. In conclusion, L. rhamnosus XN2 and its bacteriocin showed antagonistic activity at both cellular and quorum sensing (QS) levels. Finally, bacteriocin was further purified by reversed-phase high-performance liquid chromatography (RP-HPLC), named bacteriocin XN2. The amino acid sequence was Met-Lue-Lys-Lys-Phe-Ser-Thr-Ala-Tyr-Val.
A Robust Flow-Based System for the Spectrophotometric Determination of Cr(VI) in Recreational Waters
Authors: Ribas Tânia C. F. , Mesquita Raquel B. R. , Machado Ana , Miranda Joana L. A. , Marshall Graham , Bordalo Adriano , Rangel António O. S. S.
Published: March 2022

A flow-based method for the spectrophotometric determination of chromium (VI) in recreational waters with different salinities was developed. Chromium can occur in the environment in different oxidation states with different related physiological properties. With regard to chromium, the speciation is particularly important, as the hexavalent chromium is considered to be carcinogenic. To achieve that purpose, the use of the diphenylcarbazide (DPC) selective colored reaction with the hexavalent chromium was the chosen strategy. The main objective was to develop a direct and simple spectrophotometric method that could cope with the analysis of different types of environmental waters, within different salinity ranges (fresh to marine waters). The potential interference of metal ions, that can usually be present in environmental waters, was assessed and no significant interferences were observed (<10%). For a complete Cr(VI) determination (three replicas) cycle, the corresponding reagents consumption was 75 µg of DPC, 9 mg of ethanol and 54 mg of sulfuric acid. Each cycle takes about 5 min, including the system clean-up. The limit of detection was 6.9 and 12.2 µg L−1 for waters with low and high salt content, respectively. The method was applied for the quantification of chromium (VI) in both fresh and marine water, and the results were in agreement with the reference procedure.
A Comparative Study between Conventional and Advanced Extraction Techniques: Pharmaceutical and Cosmetic Properties of Plant Extracts
Authors: El Maaiden Ezzouhra , Bouzroud Sarah , Nasser Boubker , Moustaid Khadija , El Mouttaqi Ayoub , Ibourki Mohamed , Boukcim Hassan , Hirich Abdelaziz , Kouisni Lamfeddal , El Kharrassi Youssef
Published: March 2022

This study aimed to compare the influence of extraction methods on the pharmaceutical and cosmetic properties of medicinal and aromatic plants (MAPs). For this purpose, the dried plant materials were extracted using advanced (microwave (MAE), ultrasonic (UAE), and homogenizer (HAE) assisted extractions) and conventional techniques (maceration, percolation, decoction, infusion, and Soxhlet). The tyrosinase, elastase, α-amylase, butyryl, and acetylcholinesterase inhibition were tested by using L-3,4 dihydroxy-phenylalanine, N-Succinyl-Ala-Ala-p-nitroanilide, butyryl, and acetylcholine as respective substrates. Antioxidant activities were studied by ABTS, DPPH, and FRAP. In terms of extraction yield, advanced extraction techniques showed the highest values (MAE > UAE > HAE). Chemical profiles were dependent on the phenolic compounds tested, whereas the antioxidant activities were always higher, mainly in infusion and decoction as a conventional technique. In relation to the pharmaceutical and cosmetic properties, the highest inhibitory activities against α-amylase and acetylcholinesterase were observed for Soxhlet and macerated extracts, whereas the highest activity against tyrosinase was obtained with MAE > maceration > Soxhlet. Elastase and butyrylcholinesterase inhibitory activities were in the order of Soxhlet > maceration > percolation, with no activities recorded for the other tested methods. In conclusion, advanced methods afford an extract with high yield, while conventional methods might be an adequate approach for minimal changes in the biological properties of the extract.
Anti-Depressant Properties of Crocin Molecules in Saffron
Authors: Siddiqui Shahida Anusha , Ali Redha Ali , Snoeck Edgar Remmet , Singh Shubhra , Simal-Gandara Jesus , Ibrahim Salam A. , Jafari Seid Mahdi
Published: March 2022

Saffron is a valued herb, obtained from the stigmas of the C. sativus Linn (Iridaceae), with therapeutic effects. It has been described in pharmacopoeias to be variously acting, including as an anti-depressant, anti-carcinogen, and stimulant agent. The therapeutic effects of saffron are harbored in its bioactive molecules, notably crocins, the subject of this paper. Crocins have been demonstrated to act as a monoamine oxidase type A and B inhibitor. Furthermore, saffron petal extracts have experimentally been shown to impact contractile response in electrical field stimulation. Other research suggests that saffron also inhibits the reuptake of monoamines, exhibits N-methyl-d-aspartate antagonism, and improves brain-derived neurotrophic factor signaling. A host of experimental studies found saffron/crocin to be similarly effective as fluoxetine and imipramine in the treatment of depression disorders. Saffron and crocins propose a natural solution to combat depressive disorders. However, some hurdles, such as stability and delivery, need to be overcome.
Tailoring Functional Terminals on Solution-Processable Fullerene Electron Transporting Materials for High Performance Perovskite Solar Cells
Authors: Liu Fu , Xing Zhou , Ren Ya , Huang Rong-Jiao , Xu Piao-Yang , Xie Fang-Fang , Li Shu-Hui , Zhong Xinxian
Published: March 2022

Widely known as an excellent electron transporting material (ETM), pristine fullerene C60 plays a critical role in improving the photovoltaic performance of inverted structure perovskite solar cells (PSCs). However, the imperfect perovskite/C60 interface significantly limits the promotion of device performance and stability due to the weak coordination interactions between bare carbon cages and perovskite. Here, we designed and synthesized three functionalized fulleropyrrolidine ETMs (abbreviated as CEP, CEPE, and CECB), each of which was modified with the same primary terminal (cyanoethyl) and various secondary terminals (phenyl, phenethyl, and chlorobutyl). The resulting CECB-based PSC has a power conversion efficiency (PCE) over 19% and exceptional photo-stability over 1800 h. This work provides significant insight into the targeted terminal design of novel fullerene ETMs for efficient and stable PSCs.
Effect of the Carbon Support and Conditions on the Carbothermal Synthesis of Cu-Molybdenum Carbide and Its Application on CO2 Hydrogenation to Methanol
Authors: Dongil Ana Belén , Blanco Elodie , Villora-Picó Juan José , Sepúlveda-Escribano Antonio , Rodríguez-Ramos Inmaculada
Published: March 2022

The synthesis of methanol by carbon dioxide hydrogenation has been studied using copper-molybdenum carbides supported on high surface area graphite, reduced graphene oxide and carbon nanotubes. The synthesis conditions and the effect of the support were studied. The catalysts were prepared in situ using H2 or He at 600 °C or 700 °C. Both molybdenum carbide and oxycarbide were obtained. A support with less reactive carbon resulted in lower proportion of carbide obtained. The best results were achieved over a 5 wt.% Cu and 10 wt.% Mo on high surface area graphite that reached 96.3% selectivity to methanol.
Size- and Oxidation-Dependent Toxicity of Graphene Oxide Nanomaterials in Embryonic Zebrafish
Authors: Lopez Ryan M. , White Joshua R. , Truong Lisa , Tanguay Robyn L.
Published: March 2022

Graphene oxides (GOs) are a popular graphene alternative. The goal of this study was to compare the biocompatibility of a diversity of well-characterized GOs. Our previous work advanced developmental zebrafish as a model to interrogate the interactions and biological responses following exposures to engineered nanomaterials (ENMs). Here, we investigated GO 250 nm × 250 nm (sGO), 400 nm × 400 nm (mGO), and 1 μm × 1 μm (lGO), partially reduced GO (prGO) 400 nm × 400 nm, and reduced GO (rGO) 400 nm × 400 nm and 2 μm × 2 μm, which first underwent extensive characterization under the support of the Nanomaterials Health Implications Research (NHIR) Consortium. GOs were stabilized in water (GOs), while prGO and rGOs were dispersed in sodium cholate. Zebrafish were statically exposed to up to 50 μg/mL of each material from 6 h post-fertilization (hpf) until 120 hpf. Toxicity was dependent on GO properties. mGO was the most toxic material; its effects manifested in the yolk syncytial layer (YSL). Additionally, sodium cholate stabilization significantly increased GO toxicity. The observed effects were size- and oxidation-state-dependent, revealing the importance of identifying the structure-specific toxicity of GOs.
Do MAFLD Patients with Harmful Alcohol Consumption Have a Different Dietary Intake?
Authors: Policarpo Sara , Carvalhana Sofia , Craciun Ana , Crespo Ricardo Rios , Cortez-Pinto Helena
Published: March 2022

The term metabolic-associated fatty liver disease (MAFLD) has been proposed to define positively fatty liver disease in the form associated with metabolic risk factors. The aim of this study was to assess the dietary intake of MAFLD and explore a possible relationship between its inflammatory characteristics (assessed by Dietary Inflammatory Index—DII®), the degree of liver fibrosis (assessed by transient elastography), and the amount of alcohol intake. MAFLD patients were included (n = 161) and were classified, according to the amount of alcoholic intake, as MAFLD without alcohol intake (n = 77) and MAFLD with alcohol intake (n = 84), with 19 presenting harmful alcoholic consumption. Dietary intake was 1868 ± 415 kcal/day and did not present differences in energy or nutrient intake based on the presence of metabolic comorbidities. Patients with MAFLD and alcohol intake consumed significantly more energy and presented a tendency for higher intake of carbohydrates and sugar. Patients with harmful alcohol intake presented a higher intake of total fat and cholesterol compared with moderate alcohol intake. There were no differences in DII® based on fibrosis severity or the amount of alcohol consumption. This work contributes to the characterization of baseline dietary intake in MAFLD patients, paving the way to design more suited dietary interventional trials.
Effect of Evening Primrose Oil Supplementation on Biochemical Parameters and Nutrition of Patients Treated with Isotretinoin for Acne Vulgaris: A Randomized Double-Blind Trial
Authors: Kaźmierska Agnieszka , Bolesławska Izabela , Jagielski Paweł , Polańska Adriana , Dańczak-Pazdrowska Aleksandra , Kosewski Grzegorz , Adamski Zygmunt , Przysławski Juliusz
Published: March 2022

Background: Acne vulgaris is one of the most common skin diseases. One of the therapeutic options recommended for severe acne or acne that has not responded to previous therapies is isotretinoin. However, its use may lead to adverse changes in the serum lipid profile and increased levels of transaminases. In this study, we evaluated the effect of supplementation with evening primrose oil in acne vulgaris patients treated with isotretinoin on blood lipid parameters and transaminase activity. Methods: Study participants were randomly assigned to two treatments: conventional with isotretinoin (25 patients) and novel with isotretinoin combined with evening primrose oil (4 × 510 mg/day; 25 patients) for 9 months. Results: Compared to isotretinoin treatment, isotretinoin treatment combined with evening primrose oil had a positive effect on TCH concentrations (mean: 198 vs. 161, p < 0.001), LDL (95.9 vs. 60.2, p < 0.001), HDL (51.0 vs. 48.0, p < 0.001), TG (114 vs. 95.0, p < 0.001), ALT (24.0 vs. 22.0, p < 0.001), and AST (28.0 vs. 22.0, p < 0.001), but had no effect on the energy and ingredient content of the diets (p > 0.05) after treatment. Conclusion: Evening primrose oil was found to have beneficial effects on lipid profiles and transaminase activity during isotretinoin treatment. However, longer studies are needed to make more reliable decisions regarding the use of evening primrose oil and its safety in clinical practice. The evening primrose oil treatment group also showed a reduction in dietary energy due to a reduction in dietary protein and carbohydrates.
Sport Nutrition Knowledge, Attitudes, Sources of Information, and Dietary Habits of Sport-Team Athletes
Authors: Vázquez-Espino Karla , Rodas-Font Gil , Farran-Codina Andreu
Published: March 2022

Nutrition knowledge (NK) is one of several factors needed to establish proper eating habits and is especially important for athletes. The aims of this study were the following: to assess the NK of athletes from the Fútbol Club Barcelona; and to study its possible association with self-perceived level of NK, attitude towards nutrition, sources of information, and some dietary habits. We performed a cross-sectional study in two parts. First, we assessed the NK of elite athletes (n = 264) and compared it to the NK of technical teams of different sports (n = 59) and non-athletes (n = 183) of different ages and levels of education. Second, we investigated the associations between NK and other variables. To assess NK, we used a previously validated questionnaire Nutrition Knowledge Questionnaire for Young and Adult Athletes (NUKYA). Athletes showed a low median score (25.1 points), similar to the scores obtained by high school students (19.5) and university Philosophy students (29.0), and significantly lower than the scores of the sports technical team (58.5, p<0.05) and final year students of Human Nutrition and Dietetics (74.6, p<0.05). Moreover, we found statistically significant associations between NK and self-perceived level of NK (n=240,ρ=0.2546,p=0.0001) intake of fruits and vegetables (n=111,ρ=0.2701,p=0.0041), and intake of discretionary food (n=111,ρ=−0.2008,p=0.0001). Athletes with lower scores tended to overestimate their competence in nutrition (Dunning-Kruger effect). We concluded that NK of athletes needs to be improved through education plans that should consider aspects such as the proper selection of information resources and the importance of not consuming supplements without the adequate prescription. Incorporation of technical team and families to the education plan should be considered.
Colonization of Group B Streptococcus in Pregnant Women and Their Neonates from a Sri Lankan Hospital
Authors: Sapugahawatte Dulmini Nanayakkara , Li Carmen , Liyanapathirana Veranja , Kandauda Chaminda , Gihan Champika , Zhu Chendi , Lo Norman Wai Sing , Wong Kam Tak , Ip Margaret
Published: March 2022

We investigated the molecular epidemiology of Streptococcus agalactiae (Group B Streptococcus, GBS) from carriage in a cohort of pregnant mothers and their respective newborns in a Teaching Hospital in Sri Lanka. GBS vaginal carriage was assessed on pregnant mothers at pre-delivery (n = 250), post-delivery (n = 130), and from peri-rectal swabs of neonates (n = 159) in a prospective study. All colonizing, non-duplicate GBS isolates (n = 60) were analyzed for antimicrobial susceptibilities, capsular serotyping, and whole-genome sequencing (WGS). The percentage of GBS carriage in mothers in the pre-delivery and post-delivery cohorts were 11.2% (n = 28) and 19.2% (n = 25), respectively, and 4.4% (n = 7) in neonates. GBS isolates predominantly belonged to serotype VI (17/60, 28.3%). The isolates spanned across 12 sequence types (STs), with ST1 (24/60, 40%) being the most predominant ST. Concomitant resistance to erythromycin, tetracyclines, and gentamicin was observed in eight strains (13.3%). WGS revealed the presence of antimicrobial resistance genes including ermA (5/60), mefA (1/60), msrD (1/60), and tetLMO (2/60, 28/60, and 1/60, respectively) among 60 strains. The study provides insight into the diversity of vaccine targets of GBS since serotype VI is yet to be covered in the vaccine development program.
Pyoluteorin Produced by the Biocontrol Agent Pseudomonas protegens Is Involved in the Inhibition of Heterobasidion Species Present in Europe
Authors: Pellicciaro Martina , Padoan Elio , Lione Guglielmo , Celi Luisella , Gonthier Paolo
Published: March 2022

Pseudomonas protegens (strain DSMZ 13134) is a biocontrol agent with promising antagonistic activity hinging on antibiosis against the fungal forest pathogens Heterobasidion spp. Here, by using High-Performance Liquid Chromatography coupled to Mass Spectrometry (HPLC-MS), we assessed whether monocultures of P. protegens (strain DSMZ 13134) produce the three major determinants of biocontrol activity known for the genus Pseudomonas: 2,4-diacetylphloroglucinol (2,4-DAPG), pyoluteorin (PLT), and pyrrolnitrin (PRN). At the tested culture conditions, we observed the production of PLT at concentrations ranging from 0.01 to 10.21 mg/L and 2,4-DAPG at a concentration not exceeding 0.5 mg/L. Variations of culture conditions involving culture medium, incubation temperature, and incubation period had no consistent influence on PLT production by the bacterium. Assays using culture medium amended with PLT at the same concentration of that present in cell-free filtrate of the bacterium, i.e., 3.77 mg/L, previously documented as effective against Heterobasidion spp., showed a remarkable activity of PLT against genotypes of all the four Heterobasidion species present in Europe, including the non-native invasive H. irregulare. However, such antifungal activity decreased over time, and this may be a constraint for using this molecule as a pesticide against Heterobasidion spp. When the bacterium was co-cultured in liquid medium with genotypes of the different Heterobasidion species, an increased production of PLT was observed at 4 °C, suggesting the bacterium may perform better as a PLT producer in field applications under similar environmental conditions, i.e., at low temperatures. Our results demonstrated the role of PLT in the inhibition of Heterobasidion spp., although all lines of evidence suggest that antibiosis does not rely on a single constitutively produced metabolite, but rather on a plethora of secondary metabolites. Findings presented in this study will help to optimize treatments based on Pseudomonas protegens (strain DSMZ 13134) against Heterobasidion spp.
New Peptide Based Fluconazole Conjugates with Expanded Molecular Targets
Authors: Brankiewicz Wioletta , Okońska Joanna , Serbakowska Katarzyna , Lica Jan , Drab Marek , Ptaszyńska Natalia , Łęgowska Anna , Rolka Krzysztof , Szweda Piotr
Published: March 2022

Infections of Candida spp. etiology are frequently treated with azole drugs. Among azoles, the most widely used in the clinical scenario remains fluconazole (FLC). Promising results in treatment of dangerous, systemic Candida infections demonstrate the advantages of combined therapies carried out with combinations of at least two different antifungal agents. Here, we report five conjugates composed of covalently linked FLC and cell penetrating or antimicrobial peptide: TP10-7-NH2, TP10-NH2, LFcinB(2-11)-NH2, LFcinB[Nle1,11]-NH2, and HLopt2-NH2, with aspects of design, chemical synthesis and their biological activities. Two of these compounds, namely FLCpOH-TP10-NH2 and FLCpOH-TP10-7-NH2, exhibit high activity against reference strains and fluconazole-resistant clinical isolates of C. albicans, including strains overproducing drug transporters. Moreover, both of them demonstrate higher fungicidal effects compared to fluconazole. Analysis performed with fluorescence and scanning electron microscopy as well as flow cytometry indicated the cell membrane as a molecular target of synthesized conjugates. An important advantage of FLCpOH-TP10-NH2 and FLCpOH-TP10-7-NH2 is their low cytotoxicity. The IC90 value for the human cells after 72 h treatment was comparable to the MIC50 value after 24 h treatment for most strains of C. albicans. In reported conjugates, FLC was linked to the peptide by its hydroxyl group. It is worth noting that conjugation of FLC by the nitrogen atom of the triazole ring led to practically inactive compounds. Two compounds produced by us and reported herein appear to be potential candidates for novel antifungal agents.
Interaction of Antifungal Drugs with CYP3A- and OATP1B-Mediated Venetoclax Elimination
Authors: Eisenmann Eric D. , Garrison Dominique A. , Talebi Zahra , Jin Yan , Silvaroli Josie A. , Kim Jin-Gyu , Sparreboom Alex , Savona Michael R. , Mims Alice S. , Baker Sharyn D.
Published: March 2022

Venetoclax, a BCL-2 inhibitor used to treat certain hematological cancers, exhibits low oral bioavailability and high interpatient pharmacokinetic variability. Venetoclax is commonly administered with prophylactic antifungal drugs that may result in drug interactions, of which the underlying mechanisms remain poorly understood. We hypothesized that antifungal drugs may increase venetoclax exposure through inhibition of both CYP3A-mediated metabolism and OATP1B-mediated transport. Pharmacokinetic studies were performed in wild-type mice and mice genetically engineered to lack all CYP3A isoforms, or OATP1B2 that received venetoclax alone or in combination with ketoconazole or micafungin. In mice lacking all CYP3A isoforms, venetoclax AUC was increased by 1.8-fold, and pretreatment with the antifungal ketoconazole further increased venetoclax exposure by 1.6-fold, despite the absence of CYP3A. Ensuing experiments demonstrated that the deficiency of OATP1B-type transporters is also associated with increases in venetoclax exposure, and that many antifungal drugs, including micafungin, posaconazole, and isavuconazole, are inhibitors of this transport mechanism both in vitro and in vivo. These studies have identified OATP1B-mediated transport as a previously unrecognized contributor to the elimination of venetoclax that is sensitive to inhibition by various clinically-relevant antifungal drugs. Additional consideration is warranted when venetoclax is administered together with agents that inhibit both CYP3A-mediated metabolism and OATP1B-mediated transport.
On Explainable AI and Abductive Inference
Authors: Medianovskyi Kyrylo , Pietarinen Ahti-Veikko
Published: March 2022

Modern explainable AI (XAI) methods remain far from providing human-like answers to ‘why’ questions, let alone those that satisfactorily agree with human-level understanding. Instead, the results that such methods provide boil down to sets of causal attributions. Currently, the choice of accepted attributions rests largely, if not solely, on the explainee’s understanding of the quality of explanations. The paper argues that such decisions may be transferred from a human to an XAI agent, provided that its machine-learning (ML) algorithms perform genuinely abductive inferences. The paper outlines the key predicament in the current inductive paradigm of ML and the associated XAI techniques, and sketches the desiderata for a truly participatory, second-generation XAI, which is endowed with abduction.
Endogenous Hormone Profile and Sugars Display Differential Distribution in Leaves and Pseudobulbs of Laelia anceps Plants Induced and Non-Induced to Flowering by Exogenous Gibberellic Acid
Authors: Tejeda-Sartorius Olga , Soto-Hernández Ramón Marcos , San Miguel-Chávez Rubén , Trejo-Téllez Libia Iris , Caamal-Velázquez Humberto
Published: March 2022

A profile of endogenous hormones and sugars in leaves and pseudobulbs of Laelia anceps subsp. anceps (Orchidaceae) plants induced and non-induced to flowering by the effect of different doses of exogenous gibberellic acid (GA3), considering the current and back growth structures (CGS and BGS), were investigated. A factorial experiment with five doses of GA3 and two growth structures was designed. Adult plants with undifferentiated vegetative buds were selected and sprayed with doses of 0, 400, 600, 800, and 1000 mg GA3 L−1. The main results showed a strong interaction between GA3 dose and growth structures, which promoted the highest kinetin (KIN) concentration in CGS. Exogenous GA3 increased endogenous GA3 in leaves and pseudobulbs induced (I-Leaf and I-PSB) and non-induced (NI-Leaf and NI-PSB) to flowering. For sugar concentration, the 400 mg L−1 GA3 dose promotes significant interaction with the CGS in NI-PSB. In general, the hormone profile revealed opposite balances of endogenous hormone concentrations for KIN, zeatin (ZEA), trans-zeatin (T-ZEA), indoleacetic acid (IAA), indole-3-butyric acid (IBA) and GA3, not only for growth structures but also for vegetative organs analyzed, depending on whether the plants were induced or not induced to flowering, with the highest concentration of endogenous hormones in pseudobulbs. Likewise, different sugar concentration balances were observed. These balances of both endogenous hormones and sugars are likely to be involved in the flowering of L. anceps.
Antioxidant, Cytotoxic, and Rheological Properties of Canola Oil Extract of Usnea barbata (L.) Weber ex F.H. Wigg from Călimani Mountains, Romania
Authors: Popovici Violeta , Bucur Laura , Gîrd Cerasela Elena , Rambu Dan , Calcan Suzana Ioana , Cucolea Elena Iulia , Costache Teodor , Ungureanu-Iuga Mădălina , Oroian Mircea , Mironeasa Silvia , Schröder Verginica , Ozon Emma-Adriana , Lupuliasa Dumitru , Caraiane Aureliana , Badea Victoria
Published: March 2022

Usnea genus (Parmeliaceae, lichenized Ascomycetes) is a potent phytomedicine, due to phenolic secondary metabolites, with various pharmacological effects. Therefore, our study aimed to explore the antioxidant, cytotoxic, and rheological properties of Usnea barbata (L.) Weber ex F.H. Wigg (U. barbata) extract in canola oil (UBO) compared to cold-pressed canola seed oil (CNO), as a green solvent used for lichen extraction, which has phytoconstituents. The antiradical activity (AA) of UBO and CNO was investigated using UV-Vis spectrophotometry. Their cytotoxicity was examined in vivo through a brine shrimp lethality (BSL) test after Artemia salina (A. salina) larvae exposure for 6 h to previously emulsified UBO and CNO. The rheological properties of both oil samples (flow behavior, thixotropy, and temperature-dependent viscosity variation) were comparatively analyzed. The obtained results showed that UBO (IC50 = 0.942 ± 0.004 mg/mL) had a higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than CNO (IC50 = 1.361 ± 0.008 mg/mL). Both UBO and CNO emulsions induced different and progressive morphological changes to A. salina larvae, incompatible with their survival; UBO cytotoxicity was higher than that of CNO. Finally, in the temperature range of 32–37 °C, the UBO and CNO viscosity and viscoelastic behavior indicated a clear weakening of the intermolecular bond when temperature increases, leading to a more liquid state, appropriate for possible pharmaceutical formulations. All quantified parameters were highly intercorrelated. Moreover, their significant correlation with trace/heavy minerals and phenolic compounds can be observed. All data obtained also suggest a possible synergism between lichen secondary metabolites, minerals, and canola oil phytoconstituents.
Polymeric Composite Reinforced with PET Fiber Waste for Application in Civil Construction as a Cladding Element
Authors: Araujo Daniel , Azevedo Joyce , Cardoso Pollyana , Lazarus Benjamin , Morreira Matheus , Silva Lorrane , Barbosa Josiane
Published: March 2022

The construction industry contributes enormously to the high levels of carbon dioxide on the planet. For this reason, the sector has been investing in the development of new products that reduce the environmental impact. This study developed a fibrous polymeric composite using industrial residues of polyethylene terephthalate (PET) fibers for application in civil construction as a cladding element. The thermal and morphological characterization of the fiber was performed using Thermogravimetry (TG) and Scanning Electron Microscopy (SEM). Composites with 1, 3, and 5% PET fibers were obtained. Mechanical, morphological properties, chemical resistance, the effect of ultraviolet radiation and water absorption of the composites were evaluated. The results were compared to parameters established by the Brazilian standard NBR 15.575-3. Fibers had a smooth surface but with small surface defects, diameter between 20 and 30 µm and thermal stability up to 325.44 °C. The addition of 5% PET fibers resulted in an increase of more than 300% in the impact resistance of the composites, but with a reduction in the flexural strength. The mechanical and chemical resistance results met the parameters established by the standard used in the study. The degradation chamber test indicated that PET fibers suffered more from exposure to ultraviolet radiation than the polymeric matrix.
Physicochemical and Functional Properties of Modified KJ CMU-107 Rice Starches as Pharmaceutical Excipients
Authors: Kittipongpatana Ornanong S. , Kittipongpatana Nisit
Published: March 2022

Starch extracted from KJ CMU-107 rice, with amylose content of 13.4%, was modified to yield pre-gelatinized starch (PGS), carboxymethyl starch (CMS), crosslinked carboxymethyl starch (CLCMS), crosslinked starch (CLS), and hydroxypropyl starch (HPS). Their physicochemical properties were assessed in comparison with the native starch (NS), and their functional properties were then evaluated for potential use as pharmaceutical excipients. Scanning electron microscopic (SEM) images and X-ray diffraction (XRD) patterns showed that granules of all but one of the modified starches retained the native character and crystalline arrangement. The exception, PGS, exhibited extensive granular rupture, which correlated with the loss of crystallinity suggested by the amorphous halo in XRD. Energy-dispersive X-ray (EDX) data confirmed the modification by the presence of related elements. Carboxymethylation increased solubility in unheated water, while crosslinking improved swelling. All modified starches displayed improved oil absorption capacity by 17–64%, while CMS and CLCMS also exhibited significant moisture sorption at above 75% RH PGS and HPS exhibited lower gelatinization temperature (Tg) and enthalpic change (ΔH), while CLS showed higher Tg and ΔH. CMS, CLCMS, and CLS showed adequate powder flow and compactibility, qualifying as potential tablet excipients. The 5% w/v solutions of CMS, CLMS, and HPS also formed intact films with suitable tensile strength. Overall, modified starches derived from KJ CMU-107 could potentially be developed into new pharmaceutical excipients.
Vertical Alignment of Liquid Crystal on Sustainable 2,4-Di-tert-butylphenoxymethyl-Substituted Polystyrene Films
Authors: Yang DaEun , Jin Chowon , Kang Hyo
Published: March 2022

We synthesized sustainable 2,4-di-tert-butylphenoxymethyl-substituted polystyrenes (PDtBP#, # = 88, 68, 35, and 19, where # is molar percent contents of 2,4-di-tert-butylphenoxymethyl moiety), using post-polymerization modification reactions in order to study their liquid crystal (LC) alignment behaviors. In general, LC cells fabricated using polymer film with higher molar content of 2,4-di-tert-butylphenoxymethyl side groups showed vertical LC alignment behavior. LC alignment behavior in LC cell was related to the surface energy of the polymer alignment layer. For example, when the total surface energy value of the polymer layer was smaller than about 29.4 mJ/m2, vertical alignment behaviors were observed, generated by the nonpolar 2,4-di-tert-butylphenoxymethyl moiety with long and bulky carbon groups. Orientation stability was observed at 200 °C in the LC cells fabricated using PDtBP88 as the LC alignment layer. Therefore, as a natural compound modified polymer, PDtBP# can be used as a candidate LC alignment layer for environmentally friendly applications.
Study of the Fluid Passing through the Screen in the Three Products Hydrocyclone Screen (TPHS): A Theoretical Analysis and Numerical Simulation
Authors: Liu Haizeng , Yu Anghong , Lv Jintao , Wang Chuanzhen , Zhu Zaisheng , Khan Md. Shakhaoath
Published: March 2022

The three products hydrocyclone screen (TPHS), a branch of the hydrocyclone, effectively removes the fish-hook effect, which has been used in the industrial field. The current cylindrical screen in the TPHS generates the characteristic flow known as the screen underflow, which has a significant impact on device performance. To investigate the flow behaviour of the fluid passing through the screen, a combination of a dynamic analysis and a numerical simulation was used. The permeating process in the TPHS was abstracted by a simple fan mode in this work to generate the flow-rate equations and the driving-force models. The pressure difference was the driving force for the screen penetration in the ideal fluid, but it also included a viscous force in the viscous fluid. Furthermore, at the same inlet velocity, the viscous fluid had a higher flow rate than the ideal, indicating that the viscosity promoted the fluid penetration. Meanwhile, as the inlet velocity increased, the mass flow of the screen backflow increased, while the corresponding proportion first rose to a peak then dropped and then gradually stabilised. Furthermore, a flow equation for the screen underflow in the TPHS was developed, which is related to the structural parameters (the rotation radius, the length of the cylindrical screen, the aperture size, and the open-area percentage) and the process parameters (the dynamic viscosity of the fluid and the pressure difference between the feed inlet and the screen outlet).
The Political Discourse of the Church of Greece during the Crisis: An Empirical Approach
Authors: Kaltsas Spyridon , Karoulas Gerasimos , Karayiannis Yiannis , Kountouri Fani
Published: March 2022

This article presents the analysis of an empirical study that explored the political discourse of the Church of Greece (CoG) during a specific period of the Greek financial crisis, that is, from January 2015 to January 2019. The analysis is based on official texts of the CoG, reports in two daily newspapers, and the results of a quantitative content analysis. Through our analysis, we explore three main questions. The first focuses on the types of issues (‘agenda’) raised by the main representatives of the CoG; the second focuses on the framing used concerning these issues; and the third focuses on how the agenda and frames interact with regard to the CoG’s active (discursive) role as an ‘established church’ and/or a ‘public religion’. Our findings indicated that the CoG intervened in a considerable number of issues not strictly related to religion or the Greek financial crisis. Moreover, the main framing devices of the CoG’s discourse varied from identity to conflict frames, while its discourse reflected its self-understanding as both an established church and a public religion.