bluuebunny/crossref_metadata_2025 huggingface dataset:
Probing Gut Participation in Parkinson’s Disease Pathology and Treatment via Stem Cell Therapy
Authors: Lee Jea-Young , Castelli Vanessa , Sanberg Paul R. , Borlongan Cesar V.
Published: June 2023

Accumulating evidence suggests the critical role of the gut–brain axis (GBA) in Parkinson’s disease (PD) pathology and treatment. Recently, stem cell transplantation in transgenic PD mice further implicated the GBA’s contribution to the therapeutic effects of transplanted stem cells. In particular, intravenous transplantation of human umbilical-cord-blood-derived stem/progenitor cells and plasma reduced motor deficits, improved nigral dopaminergic neuronal survival, and dampened α-synuclein and inflammatory-relevant microbiota and cytokines in both the gut and brain of mouse and rat PD models. That the gut robustly responded to intravenously transplanted stem cells and prompted us to examine in the present study whether direct cell implantation into the gut of transgenic PD mice would enhance the therapeutic effects of stem cells. Contrary to our hypothesis, results revealed that intragut transplantation of stem cells exacerbated motor and gut motility deficits that corresponded with the aggravated expression of inflammatory microbiota, cytokines, and α-synuclein in both the gut and brain of transgenic PD mice. These results suggest that, while the GBA stands as a major source of inflammation in PD, targeting the gut directly for stem cell transplantation may not improve, but may even worsen, functional outcomes, likely due to the invasive approach exacerbating the already inflamed gut. The minimally invasive intravenous transplantation, which likely avoided worsening the inflammatory response of the gut, appears to be a more optimal cell delivery route to ameliorate PD symptoms.
Histone Maps in Gossypium darwinii Reveal Epigenetic Regulation Drives Subgenome Divergence and Cotton Domestication
Authors: Han Jinlei , Yu Guangrun , Zhang Xin , Dai Yan , Zhang Hui , Zhang Baohong , Wang Kai
Published: June 2023

The functional annotation of genomes, including chromatin modifications, is essential to understand the intricate architecture of chromatin and the consequential gene regulation. However, such an annotation remains limited for cotton genomes. Here, we conducted chromatin profiling in a wild allotetraploid cotton Gossypium darwinii (AD genome) by integrating the data of histone modification, transcriptome, and chromatin accessibility. We revealed that the A subgenome showed a higher level of active histone marks and lower level of repressive histone marks than the D subgenome, which was consistent with the expression bias between the two subgenomes. We show that the bias in transcription and histone modification between the A and D subgenomes may be caused by genes unique to the subgenome but not by homoeologous genes. Moreover, we integrate histone marks and open chromatin to define six chromatin states (S1–S6) across the cotton genome, which index different genomic elements including genes, promoters, and transposons, implying distinct biological functions. In comparison to the domesticated cotton species, we observed that 23.2% of genes in the genome exhibit a transition from one chromatin state to another at their promoter. Strikingly, the S2 (devoid of epigenetic marks) to S3 (enriched for the mark of open chromatin) was the largest transition group. These transitions occurred simultaneously with changes in gene expression, which were significantly associated with several domesticated traits in cotton. Collectively, our study provides a useful epigenetic resource for research on allopolyploid plants. The domestication–induced chromatin dynamics and associated genes identified here will aid epigenetic engineering, improving polyploid crops.
Vaccine-Induced Immunity Elicited by Microneedle Delivery of Influenza Ectodomain Matrix Protein 2 Virus-like Particle (M2e VLP)-Loaded PLGA Nanoparticles
Authors: Braz Gomes Keegan , Vijayanand Sharon , Bagwe Priyal , Menon Ipshita , Kale Akanksha , Patil Smital , Kang Sang-Moo , Uddin Mohammad N. , D’Souza Martin J.
Published: June 2023

This study focused on developing an influenza vaccine delivered in polymeric nanoparticles (NPs) using dissolving microneedles. We first formulated an influenza extracellular matrix protein 2 virus-like particle (M2e VLP)-loaded with poly(lactic-co-glycolic) acid (PLGA) nanoparticles, yielding M2e5x VLP PLGA NPs. The vaccine particles were characterized for their physical properties and in vitro immunogenicity. Next, the M2e5x VLP PLGA NPs, along with the adjuvant Alhydrogel® and monophosphoryl lipid A® (MPL-A®) PLGA NPs, were loaded into fast-dissolving microneedles. The vaccine microneedle patches were then evaluated in vivo in a murine model. The results from this study demonstrated that the vaccine nanoparticles effectively stimulated antigen-presenting cells in vitro resulting in enhanced autophagy, nitric oxide, and antigen presentation. In mice, the vaccine elicited M2e-specific antibodies in both serum and lung supernatants (post-challenge) and induced significant expression of CD4+ and CD8+ populations in the lymph nodes and spleens of immunized mice. Hence, this study demonstrated that polymeric particulates for antigen and adjuvant encapsulation, delivered using fast-dissolving microneedles, significantly enhanced the immunogenicity of a conserved influenza antigen.
A State-of-Art Review of the Vicious Circle of Sleep Disorders, Diabetes and Neurodegeneration Involving Metabolism and Microbiota Alterations
Authors: Versace Salvatore , Pellitteri Gaia , Sperotto Roberto , Tartaglia Sara , Da Porto Andrea , Catena Cristiana , Gigli Gian Luigi , Cavarape Alessandro , Valente Mariarosaria
Published: June 2023

In the context of neurodegenerative disorders, cognitive decline is frequently reported in older population. Recently, numerous metabolic pathways have been implicated in neurodegeneration, including signaling disruption of insulin and other glucose-regulating hormones. In fact, Alzheimer’s disease has now been considered as “type-3 diabetes”. In this review, we tried to clarify the role of sleep impairment as the third major player in the complex relationship between metabolic and neurodegenerative diseases. Altered sleep may trigger or perpetuate these vicious mechanisms, leading to the development of both dementia and type 2 diabetes mellitus. Finally, we analyzed these reciprocal interactions considering the emerging role of the gut microbiota in modulating the same processes. Conditions of dysbiosis have been linked to circadian rhythm disruption, metabolic alterations, and release of neurotoxic products, all contributing to neurodegeneration. In a future prospective, gut microbiota could provide a major contribution in explaining the tangled relationship between sleep disorders, dementia and diabetes.
Impairment of SK-MEL-28 Development—A Human Melanoma Cell Line—By the Crataeva tapia Bark Lectin and Its Sequence-Derived Peptides
Authors: Lie Kathleen Chwen Ming , Bonturi Camila Ramalho , Salu Bruno Ramos , de Oliveira Juliana Rodrigues , Bonini Galo Márcia , Paiva Patrícia Maria Guedes , Correia Maria Tereza dos Santos , Oliva Maria Luiza Vilela
Published: June 2023

Melanoma is difficult to treat with chemotherapy, prompting the need for new treatments. Protease inhibitors have emerged as promising candidates as tumor cell proteases promote metastasis. Researchers have developed a chimeric form of the Bauhinia bauhinioides kallikrein inhibitor, rBbKIm, which has shown negative effects on prostate tumor cell lines DU145 and PC3. Crataeva tapia bark lectin, CrataBL, targets sulfated oligosaccharides in glycosylated proteins and has also demonstrated deleterious effects on prostate and glioblastoma tumor cells. However, neither rBbKIm nor its derived peptides affected the viability of SK-MEL-28, a melanoma cell line, while CrataBL decreased viability by over 60%. Two peptides, Pep. 26 (Ac-Q-N-S-S-L-K-V-V-P-L-NH2) and Pep. 27 (Ac-L-P-V-V-K-L-S-S-N-Q-NH2), were also tested. Pep. 27 suppressed cell migration and induced apoptosis when combined with vemurafenib, while Pep. 26 inhibited cell migration and reduced nitric oxide and the number of viable cells. Vemurafenib, a chemotherapy drug used to treat melanoma, was found to decrease the release of interleukin 8 and PDGF-AB/BB cytokines and potentiated the effects of proteins and peptides in reducing these cytokines. These findings suggest that protease inhibitors may be effective in blocking melanoma cells and highlight the potential of CrataBL and its derived peptides.
Comparative Study of Nanoparticle Blood Circulation after Forced Clearance of Own Erythrocytes (Mononuclear Phagocyte System-Cytoblockade) or Administration of Cytotoxic Doxorubicin- or Clodronate-Loaded Liposomes
Authors: Mochalova Elizaveta N. , Egorova Elena A. , Komarova Kristina S. , Shipunova Victoria O. , Khabibullina Nelli F. , Nikitin Petr I. , Nikitin Maxim P.
Published: June 2023

Recent developments in the field of nanomedicine have introduced a wide variety of nanomaterials that are capable of recognizing and killing tumor cells with increased specificity. A major limitation preventing the widespread introduction of nanomaterials into the clinical setting is their fast clearance from the bloodstream via the mononuclear phagocyte system (MPS). One of the most promising methods used to overcome this limitation is the MPS-cytoblockade, which forces the MPS to intensify the clearance of erythrocytes by injecting allogeneic anti-erythrocyte antibodies and, thus, significantly prolongs the circulation of nanoagents in the blood. However, on the way to the clinical application of this approach, the question arises whether the induced suppression of macrophage phagocytosis via the MPS-cytoblockade could pose health risks. Here, we show that highly cytotoxic doxorubicin- or clodronate-loaded liposomes, which are widely used for cancer therapy and biomedical research, induce a similar increase in the nanoparticle blood circulation half-life in mice as the MPS-cytoblockade, which only gently and temporarily saturates the macrophages with the organism’s own erythrocytes. This result suggests that from the point of view of in vivo macrophage suppression, the MPS-cytoblockade should be less detrimental than the liposomal anti-cancer drugs that are already approved for clinical application while allowing for the substantial improvement in the nanoagent effectiveness.
Excitation of a Single Compound by Light and Ultrasound Enhanced the Long-Term Cure of Mice Bearing Prostate Tumors
Authors: Cacaccio Joseph , Durrani Farukh A. , Kumar Ishaan , Dukh Mykhaylo , Camacho Susan , Fayazi Zahra , Sumlin Adam , Kauffman Eric , Guru Khurshid , Pandey Ravindra K.
Published: June 2023

Current treatment for prostate cancer is dependent on the stages of the cancer, recurrence, and genetic factors. Treatment varies from active surveillance or watchful waiting to prostatectomy, chemotherapy, and radiation therapy in combination or alone. Although radical prostate cancer therapy reduces the advancement of the disease and its mortality, the increased disease treatment associated morbidity, erectile dysfunction, and incontinence affect the quality of life of cancer survivors. To overcome these problems, photodynamic therapy (PDT) has previously been investigated using PhotofrinTM as a photosensitizer (PS). However, Photofrin-PDT has shown limitations in treating prostate cancer due to its limited tumor-specificity and the depth of light penetration at 630 nm (the longest wavelength absorption of PhotofrinTM). The results presented herein show that this limitation can be solved by using a near infrared (NIR) compound as a photosensitizer (PS) for PDT and the same agent also acts as a sonosensitizer for SDT (using ultrasound to activate the compound). Compared to light, ultrasound has a stronger penetration ability in biological tissues. Exposing the PS (or sonosensitizer) to ultrasound (US) initiates an electron-transfer process with a biological substrate to form radicals and radical ions (type I reaction). In contrast, exposure of the PS to light (PDT) generates singlet oxygen (type II reaction). Therefore, the reactive oxygen species (ROS) produced by SDT and PDT follow two distinct pathways, i.e., type I (oxygen independent) and type II (oxygen dependent), respectively, and results in significantly enhanced destruction of tumor cells. The preliminary in vitro and in vivo results in a PC3 cell line and tumor model indicate that the tumor specificality of the therapeutic agent(s) can be increased by targeting galectin-1 and galectin-3, known for their overexpression in prostate cancer.
Identification and Validation of a Metabolism-Related Prognostic Signature Associated with M2 Macrophage Infiltration in Gastric Cancer
Authors: Liu Yunze , Zheng Haocheng , Gu Anna Meilin , Li Yuan , Wang Tieshan , Li Chengze , Gu Yixiao , Lin Jie , Ding Xia
Published: June 2023

High levels of M2 macrophage infiltration invariably contribute to poor cancer prognosis and can be manipulated by metabolic reprogramming in the tumor microenvironment. However, the metabolism-related genes (MRGs) affecting M2 macrophage infiltration and their clinical implications are not fully understood. In this study, we identified 173 MRGs associated with M2 macrophage infiltration in cases of gastric cancer (GC) using the TCGA and GEO databases. Twelve MRGs were eventually adopted as the prognostic signature to develop a risk model. In the high-risk group, the patients showed poorer survival outcomes than patients in the low-risk group. Additionally, the patients in the high-risk group were less sensitive to certain drugs, such as 5-Fluorouracil, Oxaliplatin, and Cisplatin. Risk scores were positively correlated with the infiltration of multiple immune cells, including CD8+ T cells and M2 macrophages. Furthermore, a difference was observed in the expression and distribution between the 12 signature genes in the tumor microenvironment through single-cell sequencing analysis. In vitro experiments proved that the M2 polarization of macrophages was suppressed by Sorcin-knockdown GC cells, thereby hindering the proliferation and migration of GC cells. These findings provide a valuable prognostic signature for evaluating clinical outcomes and corresponding treatment options and identifying potential targets for GC treatment.
Anti-Inflammatory Activity of Black Soldier Fly Oil Associated with Modulation of TLR Signaling: A Metabolomic Approach
Authors: Richter Hadas , Gover Ofer , Schwartz Betty
Published: June 2023

Dietary intervention in the treatment of ulcerative colitis involves, among other things, modifications in fatty acid content and/or profile. For example, replacing saturated long chain fatty acids with medium chain fatty acids (MCFAs) has been reported to ameliorate inflammation. The Black Soldier Fly Larvae’s (BSFL) oil is considered a sustainable dietary ingredient rich in the MCFA C12:0; however, its effect on inflammatory-related conditions has not been studied until now. Thus, the present study aimed to investigate the anti-inflammatory activity of BSFL oil in comparison to C12:0 using TLR4- or TLR2-activated THP-1 and J774A.1 cell lines and to assess its putative protective effect against dextran sulfate sodium (DSS)-induced acute colitis in mice. BSFL oil and C12:0 suppressed proinflammatory cytokines release in LPS-stimulated macrophages; however, only BSFL oil exerted anti-inflammatory activity in Pam3CSK4-stimulated macrophages. Transcriptome analysis provided insight into the possible role of BSFL oil in immunometabolism switch, involving mTOR signaling and an increase in PPAR target genes promoting fatty acid oxidation, exhibiting a discrepant mode of action compared to C12:0 treatment, which mainly affected cholesterol biosynthesis pathways. Additionally, we identified anti-inflammatory eicosanoids, oxylipins, and isoprenoids in the BSFL oil that may contribute to an orchestrated anti-inflammatory response. In vivo, a BSFL oil-enriched diet (20%) ameliorated the clinical signs of colitis, as indicated by improved body weight recovery, reduced colon shortening, reduced splenomegaly, and an earlier phase of secretory IgA response. These results indicate the novel beneficial use of BSFL oil as a modulator of inflammation.
Behavioral Study of 3- and 5-Halocytisine Derivatives in Zebrafish Using the Novel Tank Diving Test (NTT)
Authors: Farías-Cea Amaury , Leal Cristóbal , Hödar-Salazar Martín , Esparza Erica , Martínez-Duran Luis , Fuentes Irma , Iturriaga-Vásquez Patricio
Published: June 2023

Anxiety is a serious mental disorder, and recent statistics have determined that 35.12% of the global population had an anxiety disorder during the COVID-19 pandemic. A mechanism associated with anxiolytic effects is related to nicotinic acetylcholine receptor (nAChR) agonists, principally acting on the α4β2 nAChR subtype. nAChRs are present in different animal models, including murine and teleosteos ones. Zebrafish has become an ideal animal model due to its high human genetic similarities (70%), giving it high versatility in different areas of study, among them in behavioral studies related to anxiety. The novel tank diving test (NTT) is one of the many paradigms used for studies on new drugs related to their anxiolytic effect. In this work, an adult zebrafish was used to determine the behavioral effects of 3- and 5-halocytisine derivatives, using the NTT at different doses. Our results show that substitution at position 3 by chlorine or bromine decreases the time spent by the fish at the bottom compared to the control. However, the 3-chloro derivative at higher doses increases the bottom dwelling time. In contrast, substitution at the 5 position increases bottom dwelling at all concentrations showing no anxiolytic effects in this model. Unexpected results were observed with the 5-chlorocytisine derivative, which at a concentration of 10 mg/L produced a significant decrease in bottom dwelling and showed high times of freezing. In conclusion, the 3-chloro and 3-bromo derivatives show an anxiolytic effect, the 3-chlorocytisine derivative being more potent than the 3-bromo derivative, with the lowest time at the bottom of the tank at 1mg/L. On the other hand, chlorine, and bromine at position 5 produce an opposite effect.
Non-Vesicular Lipid Transport Machinery in Leishmania donovani: Functional Implications in Host-Parasite Interaction
Authors: Das Koushik , Nozaki Tomoyoshi
Published: June 2023

Eukaryotic cells have distinct membrane-enclosed organelles, each with a unique biochemical signature and specialized function. The unique identity of each organelle is greatly governed by the asymmetric distribution and regulated intracellular movement of two important biomolecules, lipids, and proteins. Non-vesicular lipid transport mediated by lipid-transfer proteins (LTPs) plays essential roles in intra-cellular lipid trafficking and cellular lipid homeostasis, while vesicular transport regulates protein trafficking. A comparative analysis of non-vesicular lipid transport machinery in protists could enhance our understanding of parasitism and basis of eukaryotic evolution. Leishmania donovani, the trypanosomatid parasite, greatly depends on receptor-ligand mediated signalling pathways for cellular differentiation, nutrient uptake, secretion of virulence factors, and pathogenesis. Lipids, despite being important signalling molecules, have intracellular transport mechanisms that are largely unexplored in L. donovani. We have identified a repertoire of sixteen (16) potential lipid transfer protein (LTP) homologs based on a domain-based search on TriTrypDB coupled with bioinformatics analyses, which signifies the presence of well-organized lipid transport machinery in this parasite. We emphasized here their evolutionary uniqueness and conservation and discussed their potential implications for parasite biology with regards to future therapeutic targets against visceral leishmaniasis.
Polar Codes and a M-ary Modulation-Based OFDM-PLC System
Authors: Wang Ziyi , Wang Yichen , Chen Rui
Published: June 2023

Power Line Communication (PLC) serves as a medium for communication over power lines, utilizing the existing power grid for information transmission. It offers a low-cost, highly scalable signal transmission method and has the potential to become the preferred technology for providing broadband in smart homes, offices, and smart grids. However, noise in the power line channel, especially impulse noise, seriously affects the communication quality of PLC. In this paper, we establish an OFDM-PLC system with higher-order modulation and polar codes. The higher-order QAM or APSK modulation technique is employed to increase the signal transmission rate and the system performance is analyzed. To combat the impulse noise in the PLC channel, we first model it using a superposition of multi-damping sinusoidal functions and then introduce the polar coding scheme to suppress the impulse noise in the system, thereby improving the transmission reliability. Simulation results verify that the proposed polar coding scheme based on the OFDM-PLC system can improve the Bit Error Rate (BER) performance of PLC channel transmission under impulse interference.
Nitrosyl/Diphenylphosphine/Amino Acid–Ruthenium Complexes as Inhibitors of MDA-MB-231 Breast Cancer Cells
Authors: Barbosa Marília I. F. , Corrêa Rodrigo S. , Guedes Adriana P. M. , Graça Alex M. , Andrade Francyelli M. , Leite Celisnólia M. , Silveira-Lacerda Elisângela P. , Ellena Javier , Reis Henrique V. , Doriguetto Antônio C. , Batista Alzir A.
Published: June 2023

Herein, we report on the synthesis and characterization of ruthenium compounds with the general formula [RuCl(AA-H)(NO)(dppb]PF6, where AA = glycine (1), L-alanine (2), L-phenylalanine (3) and L-valine (4), and dppb = 1,4-bis(diphenylphosphine)butane. The complexes were characterized using elemental analysis, UV/Vis and infrared spectroscopies, 1H, 13C, 31P NMR techniques, and cyclic voltammetry. Furthermore, the structures of the compounds (1) and (3) were determined using single-crystal X-ray diffraction. In vitro evaluation of the Ru(II)/nitrosyl/amino acid complexes revealed their cytotoxic activities against triple-negative MDA-MB-231 breast cancer cells, and against the non-tumor murine fibroblast cells. All the compounds decreased the percentage of viable cells, inducing cell death by apoptosis. Additionally, the Ru(II) complexes inhibited the migration of MDA-MB-231 cells at concentrations lower than 35 µM, after 48 h of exposure. Thus, these complexes may be promising agents for the treatment of triple-negative MDA-MB-231 breast cancer.
Insect Distribution in a Vacant Multi-Level Office Building
Authors: Brimblecombe Peter , Jeannottat Laure , Querner Pascal
Published: June 2023

The webbing clothes moth (Tineola bisselliella) causes extensive and costly damage to fabrics, furnishings, and museum objects. It is best known from its presence in homes, museums, and historic properties, while infestations in office buildings are not as well understood. Offices typically have more frequent cleaning, fewer quiet habitats, less food availability, and fewer breeding environments for moths, which may explain the lower abundance. Nevertheless, they can be introduced with materials or by employees whose homes have a moth infestation. This study examines the distribution of different insect pests determined from pheromone traps set out in an unoccupied multi-floor office building in Switzerland. Tineola bisselliella dominated the insect catch but was mostly found in the aisles on the lower floors. The larger rooms tended to have a greater insect catch. Carpet beetles (Attagenus smirnovi) and silverfish (Zygentoma) were also found, although in smaller numbers, and often preferred the basement floors. The ghost silverfish (Ctenolepisma calvum) dominated the Zygentoma, even though it has been rare until recently in Switzerland. The study suggests the need for Integrated Pest Management within office buildings. In addition, in unoccupied buildings under renovation, with no obvious sources of food, insect pests still need monitoring.
Energy Allocation of the Wolf Spider Pardosa pseudoannulata under Dietary Restriction
Authors: Zhu Yang , Song Li , Chen Limi , Yun Yueli , Zhang Wang , Zhao Yao , Peng Yu
Published: June 2023

The phenomenon of food shortage is widespread in spider populations, which has a great impact on their growth, development, and survival. Pardosa pseudoannulata is a dominant spider species in rice fields and has an important controlling effect on rice pests. In this study, three feeding levels were tested at the juvenile stage (H, high feeding; M, medium dietary restriction; L, severe dietary restriction) and two at the adult stage (H and L). A total of six feeding levels were tested to explore the effects of dietary restriction on the development, longevity, nutrient content, and predation by P. pseudoannulata [HH (control group), HL, MH, ML, LH, LL]. The results showed that continuous dietary restriction (ML and LL groups) had negative impacts on the growth of P. pseudoannulata and positive impacts on longevity. Spiderlings suffered from dietary restrictions during the juvenile period, and when the restrictions were removed upon reaching adulthood (MH and LH groups), their lifespan started decreasing whilst their weight began returning to normal. This suggested that there might be a trade-off between the growth and longevity of the spider under dietary restrictions. The study also found that when food was severely restricted in the juvenile stage (LH and LL groups), the nutrient contents of the adult spider could return to the same level as the control group, but the predatory ability decreased. When food was moderately restricted in the juvenile stage (MH and ML groups), the predatory ability of the adult spiders improved, while nutrients of the adult spiders declined. Our results will provide an empirical basis for the protection and effective use of dominant spider species in agricultural fields.
Practical Improvement Scenarios for an Innovative Waste-Collection Recycling Program Operating with Mobile Green Points (MGPs)
Authors: Tsimnadis Konstantinos , Kyriakopoulos Grigorios L. , Leontopoulos Stefanos
Published: June 2023

Since 2021, the prefectural authorities of the Attica Region in Greece have been operating a rewarding recycling program for the collection of clean recyclable waste in collaboration with the “Specialist Integrated Association of the Prefecture of Attica” (EDSNA, in Greek) and private contractors, called “THE GREEN CITY”. This program mobilizes almost 30 mobile green points (MGPs) daily, which are self-propelled trucks that collect clean recyclable materials from citizens and businesses across the Attica Region. After one year of operation, this program has shown promising results, having more than 100,000 registered citizens and having already collected over 500 tons of clean recyclable municipal solid wastes in more than 60 municipalities of Attica. However, these promising results are accompanied by some significant financial and environmental costs. This study presents two different practical improvement scenarios for THE GREEN CITY recycling program’s current situation that ensure (a) the shortening of the annual kilometers and time on the remote routes of all programs, (b) the annual fuel-cost decrease for the MGPs and (c) the annual reduction of their CO2 emissions into the atmosphere. Afterwards, we compare these two scenarios and conclude that the “first improvement scenario with main depot decentralization” is more realistic, feasible and has a bigger total sum of positive impacts than the second one. Therefore, this study strongly suggests the implementation of the “first improvement scenario with main depot decentralization” and opens the road to future improvement scenarios for various waste-management systems or recycling programs.
Three Doses of COVID-19 Vaccines: A Retrospective Study Evaluating the Safety and the Immune Response in Patients with Multiple Sclerosis
Authors: Maniscalco Giorgia Teresa , Di Giulio Cesare Daniele , Liguori Valerio , Manzo Valentino , Prestipino Elio , Salvatore Simona , Di Battista Maria Elena , Moreggia Ornella , Ziello Antonio Rosario , Andreone Vincenzo , Scavone Cristina , Capuano Annalisa
Published: June 2023

Since the beginning of the mass immunization of patients with multiple sclerosis (MS), many data on the efficacy and safety of COVID-19 vaccines have been produced. Considering that MS is an autoimmune disease and that some disease-modifying therapies (DMTs) could decrease the antibody response against COVID-19 vaccines, we carried out this retrospective study with the aim to evaluate the safety of these vaccines in terms of AEFI occurrence and the antibody response after MS patients had received the third dose. Two hundred and ten patients (64.8% female; mean age: 46 years) received the third dose of the mRNA-based COVID-19 vaccine and were included in the study. Third doses were administered from October 2021 to January 2022. The majority of patients (n = 193) were diagnosed with RRMS and EDSS values were ≤3.0 in 72.4% of them. DMTs most commonly used by included patients were interferon Beta 1-a, dimethyl fumarate, natalizumab and fingolimod. Overall, 160 patients (68.8% female) experienced 294 AEFIs, of which about 90% were classified as short-term, while 9.2% were classified as long-term. The most commonly reported following the booster dose were pain at the injection site, flu-like symptoms, headache, fever and fatigue. Regarding the immune response, consistently with literature data, we found that patients receiving ocrelizumab and fingolimod had lower IgG titer than patients receiving other DMTs.
Cognitive Reserve and Its Association with Cognitive and Mental Health Status following an Acute Spinal Cord Injury
Authors: Arora Mohit , Pozzato Ilaria , McBain Candice , Tran Yvonne , Sandalic Danielle , Myles Daniel , Middleton James Walter , Craig Ashley
Published: June 2023

Background: Mild cognitive impairment (MCI) is a common secondary condition associated with spinal cord injury (SCI). Cognitive reserve (CR) is believed to protect against cognitive decline and can be assessed by premorbid intelligence (pmIQ). Despite the potential utility of pmIQ as a complementary metric in the evaluation of MCI in SCI, this approach has been infrequently employed. The purpose of this study was to examine the association between MCI and pmIQ in adults with SCI with the aim of exploring the potential value of pmIQ as a marker of CR in this population. Methods: Cognitive function was assessed on three occasions in adults with SCI over a 12-month period post-injury, and pmIQ was assessed once at baseline. Demographic and mental health measures were also collected, and logistic regression was conducted to determine the strength of association between pmIQ and MCI while adjusting for factors such as mental health and age. Results: The regression analysis revealed that at the time of admission to SCI rehabilitation, the MCI assessed by a valid neurocognitive screen was strongly associated with pmIQ. That is, if a person has MCI, there was 5.4 greater odds (p < 0.01) that they will have poor pmIQ compared to a person without MCI after adjustment for age and mental health. Conclusions: The assessment of CR is an important area that should be considered to improve the process of diagnosing MCI in adults with an acute SCI and potentially facilitate earlier intervention to slow or prevent cognitive decline.
The Impact of Gender and Age in Obese Patients on Sternal Instability and Deep-Sternal-Wound-Healing Disorders after Median Sternotomy
Authors: Braun Christian , Schroeter Filip , Laux Magdalena Lydia , Kuehnel Ralf-Uwe , Ostovar Roya , Hartrumpf Martin , Necaev Anna-Maria , Sido Viyan , Albes Johannes Maximilian
Published: June 2023

Objective: The aim of this study was to investigate the relationship between age and sex in regard to the development of deep sternal wound infections and sternal instability following median sternotomy. Methods: A propensity-score-matching analysis was conducted on 4505 patients who underwent cardiac surgery between 2009 and 2021, all of whom had a BMI of ≥30 kg/m2. A total of 1297 matched pairs were determined in the sex group, and 1449 matched pairs we determined in the age group. The distributions of sex, age, diabetes mellitus, delirium, unstable sterna, wire refixation, wire removal, superficial vacuum-assisted wound closure, deep vacuum-assisted wound closure, clamp time, bypass time, logistic EuroSCORE, and BMI were determined. Results: The 30-day in-hospital mortality was found to be similar in the older and younger groups (8.149% vs. 8.35%, p = 0.947), and diabetes mellitus was also equally distributed in both groups. However, postoperative delirium occurred significantly more often in the older group (29.81% vs. 17.46%, p < 0.001), and there was a significantly higher incidence in men compared with women (16.96% vs. 26.91%, p < 0.001). There were no differences found in the incidence of sternum instability, fractured sternum, superficial vacuum-assisted wound closure, and deep vacuum-assisted wound closure between the age and sex groups. Conclusions: In conclusion, this study found that sternal instability and deep-wound-healing problems occur with equal frequency in older and younger patients and in men and women following median sternotomy. However, the likelihood of postoperative delirium is significantly higher in older patients and in men. These findings suggest that a higher level of monitoring and care may be required for these high-risk patient groups to reduce the incidence of postoperative delirium and improve outcomes following median sternotomy.
Self-Sanitizing Polycaprolactone Electrospun Nanofiber Membrane with Ag Nanoparticles
Authors: Permyakova Elizaveta S. , Manakhov Anton , Kiryukhantsev-Korneev Philipp V. , Konopatsky Anton S. , Makarets Yulia A. , Kotyakova Kristina Yu. , Filippovich Svetlana Yu. , Ignatov Sergey G. , Solovieva Anastasiya O. , Shtansky Dmitry V.
Published: June 2023

The objective of this research was to develop an environment-friendly and scalable method for the production of self-sanitizing electrospun nanofibers. This was achieved by immobilizing silver nanoparticles (Ag NPs) onto plasma-treated surfaces of biodegradable polycaprolactone (PCL) nanofibers. The plasma deposited polymer layer containing carboxyl groups played a critical role in providing a uniform distribution of Ag NPs on the nanofiber surface. Ag ions were absorbed by electrostatic interaction and then reduced under the action of UV-light. The concentration and release of Ag ions were analyzed using the EDXS/XPS and ICP AES methods, respectively. Although high levels of Ag ions were detected after 3 h of immersion in water, the material retained a sufficient amount of silver nanoparticles on the surface (~2.3 vs. 3.5 at.% as determined by XPS), and the release rate subsequently decreased over the next 69 h. The antipathogenic properties of PCL-Ag were tested against gram-negative and gram-positive bacteria, fungi, and biofilm formation. The results showed that the PCL-Ag nanofibers exhibit significant antimicrobial activity against a wide range of microorganisms, including those that cause human infections. The incorporation of Ag NPs into PCL nanofibers resulted in a self-sanitizing material that can be used in variety of applications, including wound dressings, water treatment, and air filtration. The development of a simple, scalable, and environmentally friendly method for the fabrication of these nanofibers is essential to ensure their widespread use in various industries. The ability to control the concentration and release rate of Ag ions in the PCL nanofibers will be critical to optimize their efficacy while minimizing their potential toxicity to human cells and the environment.
What Determines the Competitive Success of Young Croatian Wrestlers: Anthropometric Indices, Generic or Specific Fitness Performance?
Authors: Škugor Krešo , Gilić Barbara , Karninčić Hrvoje , Jokai Matyas , Babszky Gergely , Ranisavljev Marijana , Štajer Valdemar , Roklicer Roberto , Drid Patrik
Published: June 2023

Identifying factors influencing wrestling performance is important for determining which capacities should be developed the most. This research aimed to investigate whether anthropometric indices, generic fitness, and specific fitness performance determine the competitive success of young wrestlers. This research included 49 Croatian Greco–Roman male wrestlers aged 17.75 ± 1.51 years. Variables included training and competing experience, anthropometric indices, generic fitness (countermovement jump and handgrip strength), and specific wrestling fitness test (SWFT). Wrestlers were separated into medallists and non-medallists (i.e., wrestlers who won a medal at the previous National Championship and wrestlers who did not win a medal, respectively). The t-test for independent samples was used to determine the differences between the two categories in all variables. Moreover, discriminant analysis was performed to identify differences in a multivariate manner. Medallists and non-medallists did not differ in anthropometric indices and wrestling experience. Medallists had better results in the countermovement jump (t = 2.55, p < 0.01), handgrip strength (t = 2.77, p < 0.01), and SWFT performance (t = 2.29, p < 0.05) than non-medallists. The discriminant analysis confirmed that performance categories differed in generic and specific fitness tests (Wilks’ Lambda = 0.73, p < 0.05). It could be suggested that coaches should develop both the generic and specific fitness of their wrestlers to become more successful at competitions.
Genomic Resources for the First Federally Endangered Lichen: The Florida Perforate Cladonia (Cladonia perforata)
Authors: Leavitt Steven D. , DeBolt Ann , McQuhae Ethan , Allen Jessica L.
Published: June 2023

Thirty years after its designation as a federally endangered species, the Florida Perforate Cladonia (FPC) remains imperiled in isolated populations in the Florida scrub in the southeastern USA. For threatened and endangered species, such as FPC, reference genomes provide critical insight into genomic diversity, local adaptations, landscape-level genetics, and phylogenomics. Using high-throughput sequencing, we assemble the first draft nuclear and mitochondrial genomes for the FPC mycobiont—Cladonia perforata. We also assess genetic diversity within and among populations in southeastern Florida using genome-scale data and investigate diversity across the entire nuclear ribosomal cistron, including the standard DNA barcoding marker for fungi. The draft nuclear genome spanned 33.6 Mb, and the complete, circular mitochondrial genome was 59 Kb. We also generated the first chloroplast genome, to our knowledge, for the photobiont genus associated with FPC, an undescribed Asterochloris species. We inferred the presence of multiple, distinct mycobiont parental genotypes (genets) occurring at local scales in southeastern Florida, and strikingly, no genets were shared among even the closest sample sites. All sampled thalli shared identical mitochondrial genomes, while the nuclear ribosomal cistron showed limited variability—highlighting the genetic resolution provided by nuclear genome-scale datasets. The genomic resources generated here provide critical resources for informed conservation efforts for the FPC.
Ubiquitin Degradation of the AICAR Transformylase/IMP Cyclohydrolase Ade16 Regulates the Sexual Reproduction of Cryptococcus neoformans
Authors: Han Liantao , Wu Yujuan , Xiong Sichu , Liu Tongbao
Published: June 2023

F-box protein is a key protein of the SCF E3 ubiquitin ligase complex, responsible for substrate recognition and degradation through specific interactions. Previous studies have shown that F-box proteins play crucial roles in Cryptococcus sexual reproduction. However, the molecular mechanism by which F-box proteins regulate sexual reproduction in C. neoformans is unclear. In the study, we discovered the AICAR transformylase/IMP cyclohydrolase Ade16 as a substrate of Fbp1. Through protein interaction and stability experiments, we demonstrated that Ade16 is a substrate for Fbp1. To examine the role of ADE16 in C. neoformans, we constructed the iADE16 strains and ADE16OE strains to analyze the function of Ade16. Our results revealed that the iADE16 strains had a smaller capsule and showed growth defects under NaCl, while the ADE16OE strains were sensitive to SDS but not to Congo red, which is consistent with the stress phenotype of the fbp1Δ strains, indicating that the intracellular protein expression level after ADE16 overexpression was similar to that after FBP1 deletion. Interestingly, although iADE16 strains can produce basidiospores normally, ADE16OE strains can produce mating mycelia but not basidiospores after mating, which is consistent with the fbp1Δmutant strains, suggesting that Fbp1 is likely to regulate the sexual reproduction of C. neoformans through the modulation of Ade16. A fungal nuclei development assay showed that the nuclei of the ADE16OE strains failed to fuse in the bilateral mating, indicating that Ade16 plays a crucial role in the regulation of meiosis during mating. In summary, our findings have revealed a new determinant factor involved in fungal development related to the post-translational regulation of AICAR transformylase/IMP cyclohydrolase.
Application of Real-Time PCR Assays for the Diagnosis of Histoplasmosis in Human FFPE Tissues Using Three Molecular Targets
Authors: López Luisa F. , Tobón Ángela M. , Cáceres Diego H. , Chiller Tom , Litvintseva Anastasia P. , Gade Lalitha , González Ángel , Gómez Beatriz L.
Published: June 2023

Histoplasmosis is a fungal infection caused by the thermally dimorphic fungus Histoplasma capsulatum. This infection causes significant morbidity and mortality in people living with HIV/AIDS, especially in countries with limited resources. Currently used diagnostic tests rely on culture and serology but with some limitations. No molecular assays are commercially available and the results from different reports have been variable. We aimed to evaluate quantitative real-time PCR (qPCR) targeting three protein-coding genes of Histoplasma capsulatum (100-kDa, H and M antigens) for detection of this fungus in formalin-fixed paraffin-embedded (FFPE) samples from patients with proven histoplasmosis. The sensitivity of 100-kDa, H and M qPCR assays were 93.9%, 91% and 57%, respectively. The specificity of 100-kDa qPCR was 93% when compared against samples from patients with other mycoses and other infections, and 100% when samples from patients with non-infectious diseases were used as controls. Our findings demonstrate that real-time PCR assays targeting 100-kDa and H antigen showed the most reliable results and can be successfully used for diagnosing this mycosis when testing FFPE samples.
Effect of Ferromagnetic Metal Base on Friction and Wear of 3D-Printed Aluminum Alloy Surface under Magnetorheological Fluid Action
Authors: Li Deyong , Li Rui , Lee Chul-Hee
Published: June 2023

This study aimed to enhance the friction performance and controllable range of magnetorheological devices by investigating the impact of different materials on the tribological properties within a magnetorheological fluid (MRF) under the influence of a magnetic field. A novel friction-combined structure was proposed, consisting of a ferromagnetic metal base and a metal surface shell fabricated using 3D printing technology. The design offered several advantages: the ferromagnetic base significantly improved the magnetic field control range, the 3D-printed surface shell allowed easy replacement with different materials and textures, and it reduced both development and application costs. In this experimental study, composite samples consisting of metal 3D-printed surfaces and substrates made of different materials were used to evaluate the friction and wear characteristics of the MRF under different magnetic field conditions. Computer numerical control (CNC)-machined surfaces were also included for comparison. The results showed that the ferromagnetic matrix affected the magnetic field size and distribution of the energized coil, resulting in an increase in the friction coefficient, but also an increase in wear. Furthermore, the combination of 3D-printed surfaces with ferromagnetic substrates had a more pronounced effect on the friction coefficient compared to CNC-machined surfaces. Based on these findings, this research concluded that 3D-printed surfaces outperform CNC-machined surfaces in this specific environment. In addition, the proposed design, which combined ferromagnetic bases with 3D-printed surfaces, shows potential for improving the friction performance of friction components. The increase rate of friction coefficient from 0.1459 at no current to 0.2089 at 2.5A was 43.18%. This offers a novel application of 3D printing technology in magnetorheological devices.
Dispersal Characteristics Dependence on Mass Ratio for Explosively Driven Dry Powder Particle
Authors: Sun Binfeng , Bai Chunhua , Zhao Caihui , Li Jianping , Jia Xiaoliang
Published: June 2023

An investigation on the dispersal characteristics of the cylindrically packed material of dry powder particles driven by explosive load is presented. By establishing a controllable experimental system under laboratory conditions and combining with near-field simulation, the particle dispersal process is described. Additionally, Kelvin–Helmholtz instability is observed during the process of jet deceleration dispersal. The characteristic parameters of radially propagated particles are explored under different mass ratio of particle-to-charge (M/C). Results indicate that, when the charge mass remains constant, an increase in M/C leads to a decrease in dispersed jet number, void radius and maximum velocity, wherein the maximum velocity correlates with calculations by the porous Gurney model. The case of the smaller M/C always has a higher outer-boundary radius and area expansion factor. Findings indicate that when particles detach from the jet upon reaching minimum acceleration and entering low-speed far-field stage from high-speed near-field stage, the outer-boundary radius is 30~36 times the initial particles’ body radius under different M/C. In addition, particle concentration distribution over time and distance is qualitatively analyzed by the grayscale image method. This research can be referential for improving the fire-extinguishing capacity of extinguishing bombs and the damage property of fuel air explosive (FAE).
Analyses of L-Type Corner Joints Connected with Auxetic Dowels for Case Furniture
Authors: Kasal Ali , Smardzewski Jerzy , Kuşkun Tolga , Güray Ersan
Published: June 2023

Tests were carried out to develop and manufacture various types of auxetic dowels using 3D printing technology. These dowels were then used to connect L-type corner joint specimens for case furniture, and their strength and stiffness were analyzed through experimental, theoretical, and numerical means. In the scope of the study, eight different types of auxetic dowels including two inclusion types, two inclusion sizes, and two dowel hole diameters, as well as a reference non-auxetic dowel, were designed. Accordingly, a total of 180 specimens that included 10 replications for each group were tested; 90 were tested under tension and the remaining 90 were tested under compression. The results demonstrated that the assembly force required for the corner joints connected with auxetic dowels was significantly lower compared to non-auxetic dowels. Furthermore, the numerical and theoretical analyses yielded similar outcomes in this study. Both analyses revealed that the dowels used to connect the corner joints experienced substantial stresses during mounting and bending, ultimately leading to their failure. Upon concluding the test results, it was observed that the corner joints connected with dowels featuring rectangular inclusions exhibited superior performance when compared to those with triangular inclusions. In light of these findings, it can be concluded that further enhancements are necessary for auxetic dowels with rectangular inclusions before they can be utilized as alternative fasteners for traditional dowels.