bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of an Alternative Protocol to Study Muscle Fatigue
Authors: Alambarrio Daniela A. , Morris Benjamin K. , Davis R. Benjamin , Grabarczyk Emily B. , Turner Kari K. , Gonzalez John M.
Published: January 2025

When measuring real-time in vivo muscle fatigue with electromyography (EMG), data collection can be compromised by premature sensor removal or environmental noise; therefore, the objective of this study was to develop a postmortem in vivo methodology to induce muscle fatigue and measure it using EMG. Barrows (N = 20) were stratified by weight and randomly allocated into one of two treatments. The treatments consisted of barrows being subjected to a hog electric stunner super-contraction cycle (ES) or not (CON) postmortem. The right hind limb bicep femoris (BF) and semitendinosus (ST) were selected for ambulatory movement simulation using electronic muscle stimulation (EMS). Muscle workload during EMS was measured with EMG using median power frequency (MdPF) and root mean square (RMS) as indicators of action potential velocity and muscle fiber recruitment. Ambulatory movement was induced and recorded for 20 min with a 4:4 duty cycle at 70 Hz. Muscle biopsies were collected pre- and post-EMS for metabolite analyses to corroborate muscle fatigue onset. There was a TRT × Muscle interaction for normalized RMS percentage (p < 0.01), where BF from CON barrows had greater values (p < 0.01). There were no interactions or TRT main effects for the MdPF normalized value (p ≥ 0.25), but there were Period and muscle effects on MdPF (p < 0.01). Bicep femoris had smaller (p < 0.01) MdPF than ST. The percentage of MdPF decreased (p < 0.01) by Period 5 compared to the other Periods, which did not differ from each other (p ≥ 0.38). There were TRT × Muscle and Muscle × Period interactions for ATP muscle concentration (p ≤ 0.03). The concentration of CON BF ATP was greater (p < 0.01) than that of ES BF and CON and ES ST, which did not differ from each other (p ≥ 0.11), but the APT concentration tended to differ between ES BF and ES ST (p = 0.06). Semitendinosus ATP concentration decreased (p < 0.01) post-EMS compared to ST pre- and BF pre- and post-EMS (p ≥ 0.29), but BF and ST concentration tended to differ pre-EMS (p = 0.07). The data indicated that EMS is a valuable tool for replicating ambulatory movement or physical activity, but super-contraction is not a means to accelerate postmortem muscle fatigue onset. Therefore, further refinement, such as longer EMS stimulation time, should be considered.
Tribological and Structural Properties of Copper-Coated 3D-Printed Parts from Biodegradable Polymers
Authors: Feraru (Ilie) Mihaela , Mazurchevici Simona-Nicoleta , Lohan Nicoleta-Monica , Benchea Marcelin , Lupu Fabian Cezar , Nedelcu Dumitru
Published: January 2025

This manuscript highlights the behavior of biodegradable polymers (PLA and HD PLA Green) coated with two distinct bronze alloy powders, Metco 51F-NS (Cu 9.5Al 1.2Fe) and Metco 445 (Cu 9.5Al). The coating was realized on printed samples by using the Atmospheric Plasma Spray (APS) technique. The current investigation will explain the results related to the surface quality, micro-structure, morphology, and thermal and tribological properties. Thus, from a structural point of view, the most uniform deposition was obtained in the case of composite powder Metco 51F-NS. The thermal behavior of the samples coated with copper-based powder achieved stability up to temperatures slightly above 200 °C, with carbonization of the matrix structure taking place around 350 °C. The micro-indentation and scratch analysis responses were significantly influenced by the semicrystalline structure of the samples and the presence of the powder compounds. Based on the increased characteristics of the coated samples, the authors of the present paper consider that parts made of biodegradable polymers and coated with copper microparticles are appropriate for some applications which take place in adverse operating conditions.
The Short-Wave Infrared (SWIR) Spectral Exploration Identification and Indicative Significance of the Yixingzhai Gold Deposit, Shanxi Province
Authors: Wang Lifang , Wu Song , Lai Xiaodan , Yang Weili , Sun Rongliang , Liu Peng , Yang Yandong , Ren Yuxin
Published: January 2025

The Yixingzhai gold deposit is the largest gold deposit in Shanxi Province and develops three types of mineralization: porphyry, quartz vein, and breccia. Spectral characteristic parameters of muscovite are studied by short-wave infrared (SWIR) spectral, and the exploration significance is discussed. The Al-OH wavelength of muscovite associated with porphyry mineralization gradually becomes shorter from the periphery (>2206 nm) to the center (2201–2205 nm), and the crystallinity (>2.6) gradually increases. In quartz vein mineralization, the wavelength gradually increases from the periphery (<2203 nm) to the center (2210–2211 nm), while the crystallinity does not change significantly and in a small value (<1.5). The wavelength variation range of breccia mineralization is 2198~2214 nm and is concentrated in 2201~2204 nm near the center, while the overall crystallinity is lesser than 5.5 and concentrated around 1–2.2 near the center. The wavelength and crystallinity of muscovite are mainly affected by Tschermak substitution and temperature. When the contents of Si, Fe, and Mg are low and AlVI is high, the wavelength tends toward the short-wave (SW) direction, while the opposite tends toward the long-wave (LW) direction. The high crystallinity (4.1–8.4) of muscovite can be used as an indicator of porphyry gold mineralization and also provides an important indicator to explore similar types of gold deposits.
Chitosan and Its Nanoparticles: A Multifaceted Approach to Antibacterial Applications
Authors: Akdaşçi Emir , Duman Hatice , Eker Furkan , Bechelany Mikhael , Karav Sercan
Published: January 2025

Chitosan, a multifaceted amino polysaccharide biopolymer derived from chitin, has extensive antibacterial efficacy against diverse pathogenic microorganisms, including both Gram-negative and Gram-positive bacteria, in addition to fungi. Over the course of the last several decades, chitosan nanoparticles (NPs), which are polymeric and bio-based, have garnered a great deal of interest as efficient antibacterial agents. This is mostly due to the fact that they are used in a wide variety of applications, including medical treatments, food, chemicals, and agricultural products. Within the context of the antibacterial mechanism of chitosan and chitosan NPs, we present a review that provides an overview of the synthesis methods, including novel procedures, and compiles the applications that have been developed in the field of biomedicine. These applications include wound healing, drug delivery, dental treatment, water purification, agriculture, and food preservation. In addition to this, we focus on the mechanisms of action and the factors that determine the antibacterial activity of chitosan and its derivatives. In conjunction with this line of inquiry, researchers are strongly urged to concentrate their efforts on developing novel and ground-breaking applications of chitosan NPs.
Vitamins K2 and D3 Improve Long COVID, Fungal Translocation, and Inflammation: Randomized Controlled Trial
Authors: Atieh Ornina , Daher Joviane , Durieux Jared , Abboud Marc , Labbato Danielle , Baissary Jhony , Koberssy Ziad , Ailstock Kate , Cummings Morgan , Funderburg Nicholas , McComsey Grace
Published: January 2025

Background: Long COVID (LC) is characterized by persistent symptoms at least 3 months after a SARS-COV-2 infection. LC has been associated with fungal translocation, gut dysfunction, and enhanced systemic inflammation. Currently, there is no approved treatment for this condition. The anti-inflammatory effect of vitamins K2 and D3 was shown to help attenuate the course of acute COVID-19 infection. Objective and hypothesis: This trial aims to investigate the effects of vitamins K2/D3 on LC symptoms, as well as gut and inflammatory markers, in people with established long COVID. Our hypothesis is that by attenuating systemic inflammation, vitamins K2/D3 will improve long COVID symptoms. Methods: This single-site randomized controlled study enrolled adults experiencing ≥2 moderate LC symptoms at least 3 months after a COVID-19 infection. The RECOVER Long COVID Research Index and number and type of LC symptoms were considered. Participants were randomized 2:1 to daily 240 µg K2 (pure MK-7 form) and 2000 UI vitamin D3 or standard of care (SOC) for 24 weeks. The endpoints were changes in symptomatology and in select inflammatory, metabolic, and gut biomarkers at 24 weeks. Results: We enrolled 151 participants (n = 98 received vit K2/D3 and 53 received SOC). The median age was 46 years; 71% were female and 29% were non-white. Baseline demographics were balanced between groups. At 24 weeks, the active treatment group only had a sharp increase in 25(OH) D, indicating good treatment adherence. In the vitamin K2/D3 arm, there was a 7.1% decrease in the proportion who had an LC Index ≥12 (vs. a 7.2% increase in the SOC group; p = 0.01). The average number of LC symptoms remained stable in the vitamin K2/D3 arm but increased in the SOC arm (p = 0.03). Additionally, reductions in oxidized LDL, inflammatory markers sTNF-RI and sCD163, and fungal translocation marker (1,3)-β-d-glucan were observed in the vitamin K2/D3 arm compared to the SOC arm (p < 0.01) over 24 weeks. Conclusions: Vitamins K2/D3 improved the RECOVER Long COVID Index, the number of LC symptoms, and several gut and inflammatory markers. Vitamins K2/D3 provide a promising safe intervention for people suffering from long COVID.
Examining the Influence of Omega-3 Fatty Acids on Performance, Recovery, and Injury Management for Health Optimization: A Systematic Review Focused on Military Service Members
Authors: Rittenhouse Melissa , Khurana Saachi , Scholl Stephen , Emerson Christopher
Published: January 2025

Background/Objectives: Omega-3 fatty acids (n-3), recognized for their anti-inflammatory and brain health benefits, are being studied to enhance cognitive function, aid physical recovery, and reduce injury rates among military service members (SMs). Given the unique demands faced by this tactical population, this systematic review aims to evaluate the evidence of n-3 to support physical and mental resilience and overall performance. Methods: This review was conducted in accordance with Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines and includes articles that assessed n-3 status or implemented n-3 interventions in relation to physical and cognitive performance, recovery, and injury outcomes (2006 to 2024). Of the 1606 articles yielded in screening through Covidence, 755 were irrelevant, leaving 226 studies for full-text eligibility. Of those 226 studies, 165 studies were excluded, and 61 studies were included in this review. Results: The results highlighted evidence-based findings in five key areas where omega-3 fatty acids are being evaluated to benefit military service members. These key areas include cardiopulmonary function, exercise recovery, cognitive function, injury recovery, and strength and power. While existing research suggests promising benefits, the most significant evidence was seen with cardiopulmonary function, exercise recovery, and cognitive function. Conclusions: Current research is promising and shows potential benefits, but the results are inconclusive and inconsistent. Future research is needed to determine optimal n-3 status, dose, and possibly type of n-3 across the various performance outcomes. Understanding these gaps in research will be essential to creating evidence-based n-3 guidelines for optimal performance of SMs.
Is Intestinal Microbiota Fully Restored After Chickens Have Recovered from Coccidiosis?
Authors: Guo Jiaqing , Zhao Zijun , Broadwater Chace , Tobin Isabel , Liu Jing , Whitmore Melanie , Zhang Guolong
Published: January 2025

The intestinal microbiota is known to be altered by Eimeria-induced coccidiosis, but it remains unclear whether the microbiota is fully restored after recovery. To address this, 110 newly hatched Cobb male broiler chickens were challenged with 2 × 104 sporulated oocysts of Eimeria maxima (EM) strain M6 or mock-infected with saline on day 10. Body weight and feed intake were recorded. Additionally, 10 mock- and 12 EM-infected birds were randomly selected to assess the small intestinal lesion, fecal oocyst shedding, and ileal and cecal microbiota compositions using 16S rRNA gene sequencing at 3, 5, 7, 14, and 21 days post-infection (dpi). EM infection significantly decreased (p < 0.001) body weight by 5 dpi, persisting through 21 dpi. The infection also reduced (p < 0.05) weight gain, feed intake, and feed efficiency in the first week; however, these parameters became comparable in the second and third weeks. At 7 dpi, during the peak of infection, major lactic acid bacteria were enriched, while short-chain fatty acid-producing bacteria were mostly suppressed in both the ileum and cecum. Opportunistic pathogens such as Escherichia and Clostridium perfringens transiently bloomed at 7 dpi. By 14 dpi, differential bacterial enrichment subsided, and nearly all commensal bacteria returned to healthy levels by 21 dpi. Coupled with comparable growth performance between healthy and EM-recovered chickens, we conclude that the intestinal microbiota is largely restored to its healthy state after recovery. Understanding the microbiota’s responses to coccidiosis may inform probiotic-based mitigation strategies.
Pneumocystis Pneumonia Severity Is Associated with Taxonomic Shifts in the Respiratory Microbiota
Authors: Del Prete Valentina , Piazzesi Antonia , Scanu Matteo , Toto Francesca , Pane Stefania , Berrilli Federica , Paterno Giovangiacinto , Putignani Lorenza , di Cave David
Published: January 2025

Pneumonia caused by Pneumocystis jirovecii infection (PCP) is a potentially life-threatening illness, particularly affecting the immunocompromised. The past two decades have shown an increase in PCP incidence; however, the underlying factors that promote disease severity and fatality have yet to be fully elucidated. Recent evidence suggests that the microbiota of the respiratory tract may play a role in stimulating or repressing pulmonary inflammation, as well as the progression of both bacterial and viral pneumonia. Here, we employed 16S rRNA metataxonomic sequencing to profile the respiratory microbiota of patients with mild-moderate and severe PCP. Our results show that the upper and lower airways of PCP patients have bacterial profiles which have been associated with a pro-inflammatory response. Furthermore, we find that severe PCP is associated with lower bacterial diversity and an increase in Prevotella and a decrease in Neisseria. Functionally, severe PCP was associated with a decrease in metabolic pathways of molecules with anti-inflammatory and antimicrobial properties. To our knowledge, this is the first study showing an association of PCP severity with shifts in the respiratory microbiome and may provide some insight into which patients are more susceptible to the more severe manifestations of the disease.
A Conserved Di-Lysine Motif in the E2 Transactivation Domain Regulates MmuPV1 Replication and Disease Progression
Authors: Gonzalez Jessica , DeSmet Marsha , Androphy Elliot J.
Published: January 2025

The papillomavirus E2 protein regulates the transcription, replication, and segregation of viral episomes within the host cell. A multitude of post-translational modifications have been identified which control E2 functions. A highly conserved di-lysine motif within the transactivation domain (TAD) has been shown to regulate the normal functions of the E2 proteins of BPV-1, SfPV1, HPV-16, and HPV-31. This motif is similarly conserved in the E2 of the murine papillomavirus, MmuPV1. Using site-directed mutagenesis, we show that the first lysine (K) residue within the motif, K112, is absolutely required for E2-mediated transcription and transient replication in vitro. Furthermore, mutation of the second lysine residue, K113, to the potential acetyl-lysine mimic glutamine (Q) abrogated E2 transcription and decreased transient replication in vitro, while the acetylation defective arginine (R) mutant remained functional. Both K113 mutants were able to induce wart formation in vivo, though disease progression appeared to be delayed in the K113Q group. These findings suggest that acetylation of K113 may act as a mechanism for repressing MmuPV1 E2 activity.
Should the Definition of Low Birth Weight Be Same in Every Ethnicity Considering the DOHaD Concept?
Authors: Kasuga Yoshifumi , Tanaka Mamoru
Published: January 2025

Low birth weight (LBW) is a significant concern not only because of its association with perinatal outcomes, but also because of its long-term impact on future health. Despite the physical differences among individuals of different ethnicities, the definition of LBW remains the same for all ethnicities. This study aimed to explore and discuss this issue. We compiled national data from several countries and found that maternal height was negatively correlated with LBW incidence. We discovered the INTERGROWTH-21st chart may not be suitable for the Japanese population, as the Japanese birth weight chart differs from the INTERGROWTH-21st chart. Researchers have reported different LBW cutoff values used to assess adverse perinatal outcomes for different countries. However, there is currently no definition of LBW independent of the mother’s country of origin that can be used for predicting the risk of adverse health outcomes. Therefore, the current era of personalized healthcare may be the perfect time to establish a standard definition of LBW which is independent of the mother’s country of origin. Considering the future of healthcare, it seems an apt time to discuss the development of a more meaningful definition of LBW that can be applied across ethnicities. Further research is needed to investigate the cutoff values of LBW in every ethnicity.
Amphotericin B Encapsulation in Polymeric Nanoparticles: Toxicity Insights via Cells and Zebrafish Embryo Testing
Authors: Maciel-Magalhães Magno , Medeiros Renata Jurema , Guedes Nayara Cecília do Couto , Brito Thais Morais de , Souza Gabriele Fátima de , Canabarro Beatriz Rodrigues , Ferraris Fausto Klabund , Amendoeira Fábio Coelho , Rocha Helvécio Vinicius Antunes , Patricio Beatriz Ferreira de Carvalho , Delgado Isabella Fernandes
Published: January 2025

Background: Amphotericin B (AmB) is a commonly utilized antifungal agent, which is also recommended for the treatment of certain neglected tropical diseases, including leishmaniasis. However, its clinical application is constrained because of its poor oral bioavailability and adverse effects, prompting the investigation of alternative drug delivery systems. Polymeric nanoparticles (PNPs) have gained attention as a potential drug delivery vehicle, providing advantages such as sustained release and enhanced bioavailability, and could have potential as AmB carriers. However, concerns persist regarding nanomaterials’ toxicity, requiring more studies. Zebrafish (Danio rerio) embryos were used as a valuable model for toxicity testing, especially because of their genetic similarity to humans and standardized developmental assessments. Methods: In this study, we produced and characterized AmB loaded and non-loaded PNPs by nanoprecipitation, dynamic light scattering, transmission electron microscopy, atomic force microscopy and spectroscopy. Afterwards, we verified their toxicity through in vitro MTT assays in three cell lines (HEK293, HepG2, and J774 A1) and in vivo tests with zebrafish embryos. Results: In both trials, it was noted that nanoencapsulation of the drug led to increased toxicity when compared to non-encapsulated AmB, possibly indicating that they penetrated the embryo’s chorion. Nevertheless, it was demonstrated that the polymers used are safe and they are not the cause of toxicity, neither are the nanostructures per se. Conclusions: Therefore, it is believed that the objective of improving the bioavailability of AmB may have been achieved, and the observed toxicity was probably linked to AmB’s ability to destabilize cell membranes.
Research Progress on Applications of Metasurface-Based Optical Image Edge Detection Technology
Authors: Jiang Yuying , Sun Qingcheng , Abbas Tauseef , Ge Hongyi , Li Guangming , Jia Keke , Bu Yuwei , Zheng Huifang
Published: January 2025

With the rapid development of metasurface technology, metasurfaces have gained significant attention in optical edge detection. Owing to their precise control over the phase, amplitude, and polarization state of electromagnetic waves, metasurfaces offer a novel approach to edge detection that not only overcomes the size limitations of traditional optical devices but also significantly enhances the flexibility and efficiency of image processing. This paper reviews recent research advances in metasurfaces for optical edge detection. Firstly, the principles of phase-controlled metasurfaces in edge detection are discussed, along with an analysis of their features in different applications. Then, methods for edge detection based on polarization and dispersion modulation of metasurfaces are elaborated, highlighting the potential of these technologies for efficient image processing. In addition, the progress in multifunctional metasurfaces is presented, offering new perspectives and application prospects for future optical edge detection, along with a discussion on the limitations of metasurface-based edge detection technologies and an outlook on their future development.
Boosting Therapeutic Effect of Turmeric, Coffee, and Chili Extracts Through Experimental Design and Encapsulation as Nanostructured Lipid Carriers for Novel Heath Supplements
Authors: Tipduangta Pratchaya , Saokham Phennapha , Jiaranaikulwanitch Jutamas , Okonogi Siriporn , Ampasavate Chadarat , Kiattisin Kanokwan
Published: January 2025

This study investigates the potential synergistic effects of extracts from Curcuma longa (turmeric), Coffea arabica (Arabica coffee beans), and Capsicum annuum (chili peppers) in reducing oxidative stress and inflammation, which are associated with metabolic disorders such as obesity, diabetes, and cardiovascular diseases. Using a systematic design of experiment (DoE) optimization approach, an optimal extract ratio of 1:3:4 (turmeric: coffee: chili) was identified. The efficacy of the extract combination was assessed through various antioxidant assays, inhibition of inflammation-related gene expression, and safety testing via the 3-(4,5-dimethylthazolk-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The extract combination showed higher antioxidant activity and comparable anti-inflammatory effects relative to each single extract. Additionally, the extract combination demonstrated effective activity compared with turmeric extract while using a lower concentration, resulting in reduced cytotoxicity. The optimized extract combination was successfully incorporated into nanostructured lipid carriers (NLCs) with a hydrodynamic diameter of 258.0 ± 10.2 nm, which effectively redisperses after the spray-drying process with increased diameter to 349.8 ± 49.6 nm. Under stress conditions, the stability of curcumin and capsaicin in dried-NLCs was maintained. In summary, the optimized extract-loaded NLCs formulation, achieved through a multistage approach, shows promise in mitigating oxidative stress and inflammation, suggesting its potential as a valuable daily dietary supplement.
Nanoscale Pore Evolution of Terrestrial Shale with Thermal Maturation Level Increase Induced by Hydrous Pyrolysis
Authors: Hu Haiyan , Li Wangpeng , Yang Yifan , Chen Shuai
Published: January 2025

A series of terrestrial shale samples with different thermal maturities were subjected to hydrous artificial pyrolysis to study the evolution of terrestrial shale pores. The original shale was obtained from the terrestrial interval of a core sample, the total organic carbon (TOC) content was 8.34 wt%, and the vitrinite reflectance (Ro) was 5.31%. The original shale core was cut into eight parts, which were heated at temperatures of 300, 350, 400, 420, 450, 500, 550, and 600 °C to obtain samples with different thermal maturities. The pore size distribution (PSD), pore volume (PV), specific surface area (SSA), and pore types were investigated via CO2 and N2 adsorption tests and field emission scanning electron microscopy (FE-SEM). Many organic matter (OM) pores and mineral pores were observed via FE-SEM with increasing thermal maturity. The total PV and SSA increased until the sample reached 500 °C and then decreased, and the mesopore volume followed this trend. The micropore volume first decreased, increased until the sample reached 500 °C, and then decreased; the macropore volume increased to a peak in the sample pyrolyzed at 420 °C and then remained stable. Pores with sizes ranging from 10 to 30 nm were the predominant contributors to the shale pore volume. The SSA was affected by pores with diameters less than 20 nm, which accounted for approximately 54% of the SSA. The rate of OM conversion influenced pore creation.
Unmanned Aerial Vehicles for Real-Time Vegetation Monitoring in Antarctica: A Review
Authors: Lockhart Kaelan , Sandino Juan , Amarasingam Narmilan , Hann Richard , Bollard Barbara , Gonzalez Felipe
Published: January 2025

The unique challenges of polar ecosystems, coupled with the necessity for high-precision data, make Unmanned Aerial Vehicles (UAVs) an ideal tool for vegetation monitoring and conservation studies in Antarctica. This review draws on existing studies on Antarctic UAV vegetation mapping, focusing on their methodologies, including surveyed locations, flight guidelines, UAV specifications, sensor technologies, data processing techniques, and the use of vegetation indices. Despite the potential of established Machine-Learning (ML) classifiers such as Random Forest, K Nearest Neighbour, and Support Vector Machine, and gradient boosting in the semantic segmentation of UAV-captured images, there is a notable scarcity of research employing Deep Learning (DL) models in these extreme environments. While initial studies suggest that DL models could match or surpass the performance of established classifiers, even on small datasets, the integration of these advanced models into real-time navigation systems on UAVs remains underexplored. This paper evaluates the feasibility of deploying UAVs equipped with adaptive path-planning and real-time semantic segmentation capabilities, which could significantly enhance the efficiency and safety of mapping missions in Antarctica. This review discusses the technological and logistical constraints observed in previous studies and proposes directions for future research to optimise autonomous drone operations in harsh polar conditions.
Enhancing Human Detection in Occlusion-Heavy Disaster Scenarios: A Visibility-Enhanced DINO (VE-DINO) Model with Reassembled Occlusion Dataset
Authors: Zhao Zi-An , Wang Shidan , Chen Min-Xin , Mao Ye-Jiao , Chan Andy Chi-Ho , Lai Derek Ka-Hei , Wong Duo Wai-Chi , Cheung James Chung-Wai
Published: January 2025

Natural disasters create complex environments where effective human detection is both critical and challenging, especially when individuals are partially occluded. While recent advancements in computer vision have improved detection capabilities, there remains a significant need for efficient solutions that can enhance search-and-rescue (SAR) operations in resource-constrained disaster scenarios. This study modified the original DINO (Detection Transformer with Improved Denoising Anchor Boxes) model and introduced the visibility-enhanced DINO (VE-DINO) model, designed for robust human detection in occlusion-heavy environments, with potential integration into SAR system. VE-DINO enhances detection accuracy by incorporating body part key point information and employing a specialized loss function. The model was trained and validated using the COCO2017 dataset, with additional external testing conducted on the Disaster Occlusion Detection Dataset (DODD), which we developed by meticulously compiling relevant images from existing public datasets to represent occlusion scenarios in disaster contexts. The VE-DINO achieved an average precision of 0.615 at IoU 0.50:0.90 on all bounding boxes, outperforming the original DINO model (0.491) in the testing set. The external testing of VE-DINO achieved an average precision of 0.500. An ablation study was conducted and demonstrated the robustness of the model subject when confronted with varying degrees of body occlusion. Furthermore, to illustrate the practicality, we conducted a case study demonstrating the usability of the model when integrated into an unmanned aerial vehicle (UAV)-based SAR system, showcasing its potential in real-world scenarios.
Podcasting as an Innovative Pedagogical Tool in Social Work Education
Authors: Fox Mim , Singer Jonathan B.
Published: January 2025

Podcasting as a means of educational delivery has been slowly increasing since the introduction of podcasting to the consciousness of the general public in 2004. In parallel, social work education has experienced a global increase in online and remote delivery. Social workers globally have embraced social work content podcasts as a means of providing, and supplementing, their traditional pedagogy. In this article, the authors, both international podcasting social work academics, present an innovative framework for podcasting pedagogy and discuss co-production of podcasts as a form of teaching and learning co-design that is grounded in social work approaches to knowledge creation, dissemination, and scholarship.
The Blue School Program: A Model for Holistic Ocean Literacy Education
Authors: Costa Raquel Lorenz , Faria Cláudia
Published: January 2025

The Blue School Program is an educational initiative to enhance ocean literacy (OL) by fostering understanding and awareness of the ocean’s importance among students, teachers, and local communities. Through a holistic, interdisciplinary, and intercultural approach, it integrates scientific, cultural, and ethical dimensions of ocean education. This perspective paper examines the program’s implementation in Portugal, emphasizing its contribution to crucial educational domains such as sustainability education, scientific literacy, and citizenship education. The findings highlight notable achievements, including increased school and community engagement and the establishment of robust public-private partnerships, while also addressing challenges such as resource constraints and geographic disparities. By situating the Blue School Program within broader educational frameworks and analyzing its limitations and enablers, this paper offers insights and recommendations to support the collaborative development of Blue School networks and OL initiatives in diverse educational contexts.
‘They Only Get the Feed That Grows on Our Farm’: A Survey Experiment on Government-Subsidized Greenwashing of Swiss Meat and Agricultural Policy
Authors: Schläpfer Felix , Garibay Adriana , Ryf Stefan
Published: January 2025

Increased consumer concern about environmental issues has led to a surge in green advertising, including misleading forms known as greenwashing. Definitions of greenwashing have mostly focused on companies misleading consumers about products, and existing empirical analyses of greenwashing effects have mostly used fabricated ad materials referring to fictitious products. The objective of the present paper is to use ad material from an actual ad campaign to examine joint greenwashing, by an industry organization and a national government, of consumer products and public policies. Using an actual government-subsidized video commercial for ‘Swiss meat’ as an example, the present paper examines greenwashing targeting both consumers and citizens to influence their product choices and policy opinions. Based on an online survey of an age- and gender-representative sample (n = 637), we measured aspects of consumer knowledge that would enable the consumers to put the ad’s message in perspective, perceptions of the ad, and beliefs about Swiss meat production. Furthermore, we used a two-factorial experimental design to examine how the video commercial and objective product information affected policy opinions relative to control treatments. We find that the consumers strongly overestimated the true percentage of Swiss farms that used the environmentally friendly feeding system featured in the ad. Regarding perceptions, 52% of the respondents ‘rather’ or ‘fully’ agreed with the statement ‘the ad conveys a realistic image of Swiss meat production’, and 41% ‘rather’ or ‘fully’ agreed with the statement ‘the ad shows the production of Swiss beef as it is’. Regarding beliefs, 51% of the respondents ‘partly’, ‘rather’, or ‘fully’ agreed that ‘more meat production in Switzerland is good for the environment’. The video commercial did not influence beliefs about Swiss meat but changed policy opinions in the favor of government subsidies for the advertisement of Swiss meat. The information treatment influenced policy opinions in the opposite direction but did not decrease the effect of the misleading ad. Other significant predictors of support for increased meat subsidies included frequent meat consumption, low factual knowledge, and positive product beliefs. The results of the survey indicate that the ad campaign may have unduly influenced perceptions of Swiss meat production and public opinion about agricultural policy. The findings raise concerns about the current self-regulation of the communication industry and joint private and public advertising of Swiss agricultural products and policies.
Exploring the Mediating Role of Information Security Culture in Enhancing Sustainable Practices Through Integrated Systems Infrastructure
Authors: Hassan Yasir , Ghazal Taher M. , Yasir Saleha , Al-Adwan Ahmad Samed , Younes Sayed S. , Albahar Marwan Ali , Ahmad Munir , Ikram Atif
Published: January 2025

The need for sustainable development, coupled with the growth in industrialization, creates a complex environment in which businesses strive to achieve and maintain a competitive advantage. Information now forms a vital part of how firms perform in today’s globalized corporate world. This paper explores the impact of information systems on sustainable organizational operations. Furthermore, it observes how IT infrastructure and information security policy (ISP) play vital roles in the changing business environment. The importance of information security culture (ISC) as a mediator in developing the association between the independent and dependent variables is also investigated. Reviewing these categories’ interactions within the context of transitional economics is the main goal. To assess and predict the impact of ISs, ISP, ITI, and ISC on sustainable organizational performance (SOP), 214 businesses took part in a structured survey. For data cleaning and reliability analysis, SPSS software was used; for mediation analysis, the Preacher and Hayes approach was applied; and, for multiple linear regression analysis, Python was applied. The study is significant for developing countries in the role of IS for the effectiveness of IT governance and strategic integration. The findings indicate that organizational performance is substantially impacted by information security policy (ISP), IT infrastructure (ITI), and information security culture (ISC).
Implementing a Wide-Area Network and Low Power Solution Using Long-Range Wide-Area Network Technology
Authors: Pitu Floarea , Gaitan Nicoleta Cristina
Published: January 2025

In recent decades, technology has undergone significant transformations, aimed at optimizing and enhancing the quality of human life. A prime example of this progress is the Internet of Things (IoT) technology. Today, the IoT is widely applied across diverse sectors, including logistics, communications, agriculture, education, and infrastructure, demonstrating its versatility and profound relevance in various domains. Agriculture has historically been a fundamental sector for meeting humanity’s basic needs, and it is indispensable for survival and development. A critical factor in this regard is climatic and meteorological conditions directly influencing agricultural productivity. Therefore, real-time monitoring and analysis of these variables becomes imperative for optimizing production and reducing vulnerability to climate change. This paper presents the development and implementation of a low-power wide-area network (LPWAN) solution using LoRaWAN (long-range wide-area network) technology, designed for real-time environmental monitoring in agricultural applications. The system consists of energy-efficient end nodes and a custom-configured gateway, designed to optimize data transmission and power consumption. The end nodes integrate advanced sensors for temperature, humidity, and pressure, ensuring accurate data collection.
Food-Induced Adverse Reactions: A Review of Physiological Food Quality Control, Mucosal Defense Mechanisms, and Gastrointestinal Physiology
Authors: Guo Dongdong , Liu Chang , Zhu Hongkang , Cheng Yuliang , Huo Xiang , Guo Yahui , Qian He
Published: January 2025

Although food is essential for the survival of organisms, it can also trigger a variety of adverse reactions, ranging from nutrient intolerances to celiac disease and food allergies. Food not only contains essential nutrients but also includes numerous substances that may have positive or negative effects on the consuming organism. To protect against potentially harmful components, all animals have evolved defense mechanisms, which are similar to antimicrobial defenses but often come at the cost of the organism’s health. When these defensive responses are exaggerated or misdirected, they can lead to adverse food reactions, where the costs outweigh the benefits. Furthermore, due to the persistent toxicity of harmful food components, the failure of defense mechanisms can also result in pathological effects triggered by food. This article review presents a food quality control framework that aims to clarify how these reactions relate to normal physiological processes. Organisms utilize several systems to coexist with symbiotic microbes, regulate them, and concurrently avoid, expel, or neutralize harmful pathogens. Similarly, food quality control systems allow organisms to absorb necessary nutrients while defending against low-quality or harmful components in food. Although many microbes are lethal in the absence of antimicrobial defenses, diseases related to microbiome dysregulation, such as inflammatory bowel disease, have significantly increased. Antitoxin defenses also come with costs and may fail due to insufficiencies, exaggerations, or misdirected actions, ultimately leading to adverse food reactions. With the changes in human diet and lifestyle, the failure of defense mechanisms has contributed to the rising incidence of food intolerances. This review explores the mechanisms of antitoxin defenses and analyzes how their failure can lead to adverse food reactions, emphasizing the importance of a comprehensive understanding of food quality control mechanisms for developing more effective treatments for food-triggered diseases.
Economic and Clinical Benefits of Bivalent Respiratory Syncytial Virus Prefusion F (RSVpreF) Maternal Vaccine for Prevention of RSV in Infants: A Cost-Effectiveness Analysis for Mexico
Authors: Huerta José Luis , Kendall Robyn , Ivkovic Luka , Molina Carlos , Law Amy W. , Mendes Diana
Published: January 2025

Background/Objectives: Respiratory syncytial virus (RSV) is a leading cause of respiratory infections in children. A novel RSVpreF vaccine for use among pregnant women for the prevention of RSV in infants is expected to be licensed in Mexico. Hence, the clinical and economic burden of RSV among infants in Mexico, with and without a year-round RSVpreF maternal vaccination program, was estimated. Methods: A cohort model was developed to project clinical and economic outcomes of RSV from birth to 1 year of age for maternal vaccination and no intervention. Incremental cost-effectiveness ratios were calculated from direct cost outcomes, life years, and quality-adjusted life years (QALYs). The value per dose of the RSVpreF for which the program would be cost-effective was explored. Analyses were conducted from the healthcare system perspective, with direct costs (2024 Mexican Pesos [MXN]) and outcomes discounted at 5% annually; scenario and sensitivity analyses tested the robustness of model settings and inputs. Results: Compared to no intervention, a year-round RSVpreF vaccine administered to 1891 M pregnant women would prevent 15,768 hospitalizations, 5505 emergency department cases, and 5505 physician office visits annually, averting MXN 1754 M in direct medical costs with an increase of 3402 life years or 3666 QALYs. The RSVpreF vaccine would be cost-saving up to MXN 1301/dose and cost-effective up to MXN 2105–MXN 3715/dose under an assumed cost-effectiveness threshold range of 1–3× the gross domestic product (GDP) per capita (MXN 247,310) per QALY gained. Conclusions: Year-round RSVpreF maternal vaccination would substantially reduce RSV’s clinical and economic burden among infants in Mexico and likely be a cost-effective program.
Effects of Chlorogenic Acid on In Vitro Maturation and Vitrification Cryopreservation of Sheep Oocytes
Authors: Tao Hong , Zhao Yukun , Zhang Qiang , Li Xu , Hu Guangdong , Wang Yanping , Zeng Weibin
Published: January 2025

Chlorogenic acid (CGA) has strong antioxidant properties. In order to improve the low maturation rate and poor vitrification freezing effect of sheep oocytes caused by oxidative stress. In this study, oocytes from 200 2–3-year-old Kazakh sheep were collected, and different concentrations of CGA were added to the maturation medium and vitrification freezing solution to study the effects of CGA on the maturation rate, cleavage rate, blastocyst rate, reactive oxygen species (ROS) and glutathione (GSH) levels, mitochondrial membrane potential, and the expression levels of oxidation and apoptosis-related genes in sheep oocytes. The results showed that adding 40 μmol/L CGA to the oocyte in vitro maturation solution significantly increased the maturation rate of oocytes, adding 50 μmol/L CGA to the vitrification cryopreservative solution significantly increased the cleavage and blastocyst rates of mature oocytes activated by parthenogenetic activation after freezing. During in vitro maturation and vitrification freezing in sheep oocytes, CGA significantly reduced the level of ROS and the expression of apoptosis-related genes (Caspase-3 and Bax/Bcl-2), and significantly increased the level of glutathione (GSH), mitochondrial membrane potential, and the expression of antioxidant and anti-apoptosis-related genes (SOD-2 and GPX-3). In addition, CGA significantly increased the expression of the anti-apoptotic gene (AKT) and anti-stress gene (FOXO) during vitrification freezing of sheep oocytes. In conclusion, 40 μmol/L CGA improves the maturation rate of sheep oocytes, and 50 μmol/L CGA improves the quality of parthenogenetic activation embryos after vitrification freezing of mature oocytes in sheep. These results provide a basis for the production of sheep in vitro embryos and the establishment of a germplasm resource bank.
Integrating Field Data and Modeling for Sustainable Wastewater Irrigation Management: Case Studies from Jordan and Palestine
Authors: Aziz Rodolphe , Dragonetti Giovanna , Khadra Roula
Published: January 2025

Water shortages, overexploitation, and sectoral conflicts have prompted the use of treated wastewater (TWW) in agriculture. While TWW provides essential nutrients, improper management can harm the soil and crops. To address this, case studies from Jordan and Palestine—where alfalfa and citrus crops are exclusively irrigated with TWW—were conducted to identify suitable irrigation schedules and assess adverse impacts on crops and soils. The Safe Irrigation Management (SIM) model was used to simulate irrigation in 2021, considering TWW quality, quantity, and initial soil conditions. Two scenarios were examined: FARMOD, based on farmers’ planning, and ON-DEMAND, suggested by SIM. The results showed significant differences in irrigation frequencies and volumes between the two scenarios. The ON-DEMAND scenario demonstrated improved nitrogen and phosphorus uptake, lower soil electrical conductivity (ECe 1.5 dS·m−1), and reduced Escherichia coli (E. coli) levels (4 log10 CFU·g−1). A hypothetical scenario assuming initial soil conditions prior to TWW use yielded even lower ECe (0.8 dS·m−1) and E. coli (3.3 log10 CFU·g−1). Sensitivity analysis identified ECe and nitrogen as crucial water quality indicators requiring continuous monitoring. Integrating field data and modeling practices is vital to maintaining soil quality, supporting long-term TWW reuse especially where it is a widely adopted irrigation solution.
Treatment of Wastewater Using a Magnetically Recoverable Ag-Based Photocatalyst
Authors: Sadhwani Alonso J. Jaime , Vaswani Reboso Jenifer , Santiago Dunia E.
Published: January 2025

A novel catalyst, composed of silver phosphate (Ag3PO4) deposited on a magnetic material containing titanium dioxide (TiO2) and ferromagnetite (Fe3O4), was synthesized and tested with wastewater from the maintenance system of a swimming pool and from the secondary effluent of a wastewater treatment plant (WWTP). The magnetic material Ag3PO4@1-TiO2-Fe3O4 presented a slightly lower photoactivity than bare Ag3PO4 but was able to remove over 64% COD from the secondary effluent of a WWTP and over 75% organic UV filters from a swimming pool wastewater. The material was easily recovered from the treated water with a magnet and could be reused at least four times. The efficiency loss after five reuses was 15%. The dissolution of silver after the photocatalytic reaction was over 80 mg·L−1 for bare Ag3PO4 and less than 500 μg·L−1 for the magnetic composites. The magnetic support prevents the dissolution of silver. The materials that contained Ag3PO4 showed antibacterial properties under dark conditions. The photocatalytic efficiency of Ag3PO4 and commercial TiO2 was similar under sunlight, both for the removal of UV filters at µg·L−1 concentrations from swimming pool wastewater, and for the removal of mild COD loads from a WWTP effluent (initial COD: 110 mg·L−1).