bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of NaOH Activation on Adsorptive Removal of Herbicides by Biochars Prepared from Ground Coffee Residues
Authors: Lee Yong-Gu , Shin Jaegwan , Kwak Jinwoo , Kim Sangwon , Son Changgil , Cho Kyung Hwa , Chon Kangmin
Published: February 2021

In this study, the adsorption of herbicides using ground coffee residue biochars without (GCRB) and with NaOH activation (GCRB-N) was compared to provide deeper insights into their adsorption behaviors and mechanisms. The physicochemical characteristics of GCRB and GCRB-N were analyzed using Brunauer–Emmett–Teller surface area, Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction and the effects of pH, temperature, ionic strength, and humic acids on the adsorption of herbicides were identified. Moreover, the adsorption kinetics and isotherms were studied. The specific surface area and total pore volume of GCRB-N (405.33 m2/g and 0.293 cm3/g) were greater than those of GCRB (3.83 m2/g and 0.014 cm3/g). The GCBR-N could more effectively remove the herbicides (Qe,exp of Alachlor = 122.71 μmol/g, Qe,exp of Diuron = 166.42 μmol/g, and Qe,exp of Simazine = 99.16 μmol/g) than GCRB (Qe,exp of Alachlor = 11.74 μmol/g, Qe,exp of Diuron = 9.95 μmol/g, and Qe,exp of Simazine = 6.53 μmol/g). These results suggested that chemical activation with NaOH might be a promising option to make the GCRB more practical and effective for removing herbicides in the aqueous solutions.
Captures of Emerald Ash Borer (Agrilus planipennis) Adults in Post-Invasion White Ash Sites with Varying Amounts of Live Phloem
Authors: Robinett Molly A. , Poland Therese M. , McCullough Deborah G.
Published: February 2021

Emerald ash borer (EAB), (Agrilus planipennis Fairmaire), first identified in 2002 in southeast Michigan, has caused catastrophic ash (Fraxinus spp.) mortality in forests within the core of the invasion and has spread to 35 states and five Canadian provinces. Little is known about persistence and densities of EAB populations in post-invasion sites after most ash trees have died. We monitored EAB populations from 2014 to 2016 using double decker (DD) traps set in the midst of white ash (F. americana) trees in 30 post-invasion sites in southeast and south-central Michigan. Two DD traps were deployed at each site. One trap had a dark green upper prism and light purple lower prism, both baited with cis-3-hexenol lures. The other had two dark purple prisms baited with cis-3-hexenol on the upper prism and Manuka oil on the lower prism. In 2014 and 2016, size and condition of ash trees were recorded and area of live white ash phloem was estimated in an 18-m-radius plot centered around each of the DD traps. Area of live white ash phloem per site ranged from approximately 24 to 421 m2 in 2014 and from 24 to 411 m2 in 2016. Canopy condition of live white ash trees generally improved; 65% and 89% of the trees had healthy canopies (<20% dieback) in 2014 and 2016, respectively. Traps in 28, 29 and 30 of the sites captured a total of 580, 585, and 932 EAB adults in 2014–2016, respectively. Area of live ash phloem explained relatively little of the variation in total EAB captures in all three years. Low trap catches, along with relatively stable canopy conditions and continued abundance of live white ash, indicate that EAB populations remain below the carrying capacity of the sites, and ash phloem availability is not a limiting factor for EAB abundance. Further monitoring to track both EAB dynamics and tree condition is needed to determine the long-term outlook for white ash in these sites.
The Effect of LiDAR Sampling Density on DTM Accuracy for Areas with Heavy Forest Cover
Authors: Cățeanu Mihnea , Ciubotaru Arcadie
Published: February 2021

Laser scanning via LiDAR is a powerful technique for collecting data necessary for Digital Terrain Model (DTM) generation, even in densely forested areas. LiDAR observations located at the ground level can be separated from the initial point cloud and used as input for the generation of a Digital Terrain Model (DTM) via interpolation. This paper proposes a quantitative analysis of the accuracy of DTMs (and derived slope maps) obtained from LiDAR data and is focused on conditions common to most forestry activities (rough, steep terrain with forest cover). Three interpolation algorithms were tested: Inverse Distance Weighted (IDW), Natural Neighbour (NN) and Thin-Plate Spline (TPS). Research was mainly focused on the issue of point data density. To analyze its impact on the quality of ground surface modelling, the density of the filtered data set was artificially lowered (from 0.89 to 0.09 points/m2) by randomly removing point observations in 10% increments. This provides a comprehensive method of evaluating the impact of LiDAR ground point density on DTM accuracy. While the reduction of point density leads to a less accurate DTM in all cases (as expected), the exact pattern varies by algorithm. The accuracy of the LiDAR-derived DTMs is relatively good even when LiDAR sampling density is reduced to 0.40–0.50 points/m2 (50–60 % of the initial point density), as long as a suitable interpolation algorithm is used (as IDW proved to be less resilient to density reductions below approximately 0.60 points/m2). In the case of slope estimation, the pattern is relatively similar, except the difference in accuracy between IDW and the other two algorithms is even more pronounced than in the case of DTM accuracy. Based on this research, we conclude that LiDAR is an adequate method for collecting morphological data necessary for modelling the ground surface, even when the sampling density is significantly reduced.
Automated Marker-Free Registration of Multisource Forest Point Clouds Using a Coarse-to-Global Adjustment Strategy
Authors: Zhang Wuming , Shao Jie , Jin Shuangna , Luo Lei , Ge Junling , Peng Xinyue , Zhou Guoqing
Published: February 2021

Terrestrial laser scanning (TLS) and unmanned aerial vehicles (UAVs) are two effective platforms for acquiring forest point clouds. TLS has an advantage in the acquisition of below-canopy information but does not include the data above the canopy. UAVs acquire data from the top viewpoint but are confined to the information above the canopy. To obtain complete forest point clouds and exploit the application potential of multiple platforms in large-scale forest scenarios, we propose a practical pipeline to register multisource point clouds automatically. We consider the spatial distribution differences of trees and achieve the coarse alignment of multisource point clouds without artificial markers; then, the iterative closest point method is used to improve the alignment accuracy. Finally, a graph-based adjustment is designed to remove accumulative errors and achieve the gapless registration. The experimental results indicate high efficiency and accuracy of the proposed method. The mean errors for the registration of multi-scan TLS point clouds subsampled at 0.03 m are approximately 0.01 m, and the mean errors for registration of the TLS and UAV data are less than 0.03 m in the horizontal direction and approximately 0.01 m in the vertical direction.
The Influence of Forest Management and Changed Hydrology on Soil Biochemical Properties in a Central-European Floodplain Forest
Authors: Valtera Martin , Volánek Jiří , Holík Ladislav , Pecina Václav , Novotná Jitka , Slezák Vojtěch , Juřička David
Published: February 2021

Anthropogenic modifications to water regimes are one of the main factors threatening the stability and existence of floodplain forests. This study presents an analysis of topsoil biogeochemistry within three floodplain forest stands with different levels of human alteration. Decreasing contents of soil organic carbon (OC) and microbial biomass were observed along the gradient from natural to plantation forest. High annual variations were observed in soil N contents and in microbial biomass, while comparable spatial variations were observed within the natural forest. High ground-water levels resulted in increased accumulation of available Na+ and SO42− in the natural forest soil, yet the concentrations of ions were at sub-saline levels. The increasing contents of available Mn, SO42− or Cl− had mostly positive effects on soil microbial activity across the sites, though the results indicate the existence of a certain ecological limit for soil microorganisms. Reintroduction of surface-water flooding should be considered in future forest and water management to promote the dilution of ions accumulated in soils and natural deposition of sediments rich in organic matter (OM) at the sites.
Partial Harvest in Paludified Black Spruce Stand: Short-Term Effects on Water Table and Variation in Stem Diameter
Authors: Roy Proulx Samuel , Jutras Sylvain , Leduc Alain , Mazerolle Marc J. , Fenton Nicole J. , Bergeron Yves
Published: February 2021

The boreal forest is considered to be a low productivity forest due to its cold climate and poorly drained soils promoting paludification. These factors create conditions favouring accumulation of undecomposed organic matter, which causes declining growth rates of forest stands, ultimately converting mature stands into peatlands. Under these conditions, careful logging is conducted during winter, which minimizes soil disturbance in northwestern Quebec boreal forest. This results in water table rise, increased light availability and paludification. Our main objective was to evaluate the short-term effect of partial harvesting as an alternative method to careful logging in winter to mitigate water table rise on black spruce (Picea mariana [Mill.] B.S.P.) stands. We quantified tree stem diameter variation and daily variation in water table depth in mature spruce stands before and after partial harvest (basal area reduction of 40%) and girdling (same basal area reduction with delayed mortality) during 2016 and 2017 growing seasons. Water table variation prior to and following silvicultural treatments did not differ one year after treatment. Daily stem diameter variation in black spruce did not differ between treatments and control. Furthermore, temperature exerted a positive effect on variation in water table and on stem diameter. These results suggest that partial harvest could be more effective than clearcutting to mitigate negative effects of a high water table while limiting paludification.
Use of Post-Extraction Fir Wood Greenery Residues by the Bioconversion Method with the Production of Feed Additives
Authors: Mamaeva Olga O. , Isaeva Elena V.
Published: February 2021

The effectiveness of forest resources depends on the comprehensiveness and rationality of their consumption and processing into finished products. This article discusses the problem of using solid fir wood greenery residues generated during the industrial production of essential oils. Bioconversion is considered to be the most promising use. The objective of this research was to study the chemical composition of bioconversion products of fir wood greenery-based substrates. The PP-3.2 strain of Pleurotus pulmonarius (Fr.) Quél was used as a biodestructor. In the process of bioconversion, the contents of polysaccharides and lignin substances is reduced to 38% and 28%, respectively. Up to 20% of protein accumulates in bioconversion products of fir wood greenery. The amount of nucleic acids is not more than 1.5 g per kg; the contents of heavy metals, such as mercury, cadmium, arsenic, and lead, do not exceed the maximum permissible concentration standards. The substrate weight loss reaches 15%. When fallen leaves and post-extraction poplar bud residues are added to the substrate, the substrate-destroying activity of fungi increases, and the protein content increases by 3%. The digestibility of products as a result of bioconversion increases 1.6–2.8 times depending on the substrate composition. The obtained data support the recommendation of post-fermented substrates based on fir wood greenery and balsam poplar biomass for use as a protein feed additive.
Application of Autochthonous Yeast Saccharomyces cerevisiae XG3 in Treixadura Wines from D.O. Ribeiro (NW Spain): Effect on Wine Aroma
Authors: Blanco Pilar , Vázquez-Alén María , Garde-Cerdán Teresa , Vilanova Mar
Published: February 2021

Yeast plays an essential role in winemaking. Saccharomyces cerevisiae strains involved in fermentation determine the chemical and sensory characteristics of wines. S. cerevisiae XG3, isolated in Galicia (NW Spain), has desirable oenological potential, which has been proved at a pilot scale to produce quality wines. This study applies XG3 as active dry yeast at an industrial scale for Treixadura wine elaboration, and compares it with commercial yeast and spontaneous fermentation within three wineries included in Denomination of Origin Ribeiro over two vintages. Fermentations are monitored using conventional methods, and microbiological implantation controls are carried out by mtDNA-RFLPs analysis. Wine basic chemical parameters are determined using OIV official methodology, and volatile aroma compounds are determined by GC-MS. Finally, wine sensory analysis is also performed. S. cerevisiae XG3 shows an acceptable implantation ability—as compared to commercial control strains. The wines from XG3 have a higher total acidity and lower alcohol content. Their volatile composition differs from control wines, since XG3 produces significantly higher concentrations of acetates, volatile acids, esters and volatile phenols, depending on the vintage and winery. However, lower differences are perceived at the sensory level, where fruity and floral descriptors are perceived by the panellists in XG3 wines. Therefore, XG3 constitutes an alternative to differentiate Treixadura wines.
Three Steps Mixed (Fire Tube–Water Tube) Vertical Boiler to Optimize Thermal Performance
Authors: Aguilar Vizcarra Duilio , Esenarro Doris , Rodriguez Ciro
Published: February 2021

The research aims to design and construct a new mixed vertical boiler (fire tube – water tube) with three gas passes. The strength of this technological innovation is in the best use of the thermic transmission receiving fluid (hot water, steam, thermal oil), this due to its multipurpose function of three steps using alternative fuels (Diesel, Liquid Petroleum Gas LPG, natural gas), by improving the thermal efficiency of the boiler its temperature is reduced with gases at low temperatures, which in turn also reduce environmental pollution. The methodology focuses on calculating the transfer area with the calculation method that will allow dimensioning the boiler, considering the calculation of losses and the fluid speed, with two defined procedures, the first for fire tube and water tube boilers. And another alternative. The results obtained allowed optimizing the thermal efficiency level, achieving very significant thermal efficiency results: With LPG 92.4% for hot water and 92.42% to generate steam in the same way with natural gas 90.25% for hot water and 90.24% to generate steam as well with Diesel 2; 89.21% for hot water and 89.31% to generate steam.
Impact of Preharvest and Postharvest on Color Changes during Convective Drying of Mangoes
Authors: Diop Alioune , Méot Jean-Michel , Léchaudel Mathieu , Chiroleu Frédéric , Ndiaye Nafissatou Diop , Mertz Christian , Cissé Mady , Chillet Marc
Published: February 2021

The purpose of this study was to evaluate the impact of the harvest stage, ripening conditions and maturity on color changes of cv. ‘Cogshall’ and cv. ‘Kent’ variety mangoes during drying. A total of four harvests were undertaken, and the fruits were ripened at 20 and 35 °C for five different ripening times at each temperature. At each ripening time, mangoes were dried at 60 °C/30% RH/1.5 m/s for 5 h. A wide physico-chemical and color variability of fresh and dry pulp was created. The relationships according to the L*, H* and C* coordinates were established using mixed covariance regression models in relation to the above pre- and postharvest (preprocess) parameters. According to the L* coordinate results, browning during drying was not affected by the preprocess parameters. However, dried slices from mangoes ripened at 35 °C exhibited better retention of the initial chroma, and had a greater decrease in hue than dried slices from mangoes ripened at 20 °C. However, fresh mango color, successfully managed by the pre- and postharvest conditions, had more impact on dried mango color than the studied parameters. The preprocess parameters were effective levers for improving fresh mango color, and consequently dried mango color.
Nutritional Viewpoints on Eggs and Cholesterol
Authors: Sugano Michihiro , Matsuoka Ryosuke
Published: February 2021

Although most current epidemiologic studies indicate no significant association between consuming one egg daily and blood cholesterol levels and cardiovascular risk, arguments still persist with a positive association. Since the diet is one of the most influential factors for this association, we illustrate characteristic features in Japanese people whose dietary pattern is distinct from that, for example, the US (United States) population. Available epidemiologic studies in healthy Japanese people show no association between consumption of one egg daily and blood cholesterol level, consistent with those observed in the US population. However, when consumption of major nutrients and food sources of cholesterol are compared to the US population, Japanese people may have an extra-reserve against the influence of eggs on cardiovascular risk markers, despite consuming relatively more eggs. Further discussion on the influence of nutrients contained in the egg and dietary pattern, including interaction with gut microbes, is necessary. In addition, special consideration at the personalized level is needed for judgment regarding dietary cholesterol not only for hypercholesterolemic patients but for hyper-responsive healthy persons. Although randomized controlled trials with long-term follow-up are required to evaluate the association between consumption of eggs and human health, available information, at least from the nutritional viewpoint, suggests that egg is a healthy and cost-efficient food worldwide.
Transformation of the Food Sector: Security and Resilience during the COVID-19 Pandemic
Authors: Boyacι-Gündüz Cennet Pelin , Ibrahim Salam A. , Wei Ooi Chien , Galanakis Charis M.
Published: February 2021

The ongoing COVID-19 pandemic has resulted in a new era in the efficacy of the food supply chain, while the consequences of this new era on humanity, the economy, and the food sector are still under examination. For example, food security is one vital aspect of food systems which is directly affected. This review summarizes food security during epidemics and pandemics before moving on to panic buying, food shortages, and price spikes observed during the current crisis. The importance of food resilience, together with the need for addressing issues related to food loss and food waste, is underlined in the review towards food security and sustainable development. As a result, the pandemic has shown that our food systems are fragile. Since the global population and urbanization will grow in the coming decades, pandemics will likely occur more often, and climate change will intensify. Consequently, there is a need to ensure that our food systems become more sustainable and resilient. To that end, we have highlighted the need to develop contingency plans and mitigation strategies that would allow a more rapid response to extreme events (e.g., disasters from climate change) and transform the food sector by making it more resilient.
The Effect of Microfluidization Pressure and Tocopherol Content on the Retention of Vitamin A in Oil-In-Water Emulsions
Authors: Banasaz Shahin , Morozova Ksenia , Ferrentino Giovanna , Scampicchio Matteo
Published: February 2021

This work investigates the oxidative stability of vitamin A encapsulated in oil-in-water emulsions, which were prepared by using a microfluidizer. All emulsions were prepared with a fixed content of vitamin A (525 µM), corn oil (10%), water (90%), and whey protein (2%), but varying two main factors: the microfluidizer pressure (10, 50, 100, 200 MPa) and the amount of α-tocopherol (0, 0.25, 0.50, 1.00 mg/g). The content of vitamin A before and after the microfluidization process, and during the subsequent five weeks of storage at 40 °C were determined by HPLC-DAD. The results of the analysis of variance performed either on the data obtained before and after the microfluidization process or during the storage showed that the highest stability of vitamin A was obtained with the highest content of α-tocopherol and with an applied pressure between 100 and 200 MPa. The highest stability was explained by the smaller particle size of the resulting oil droplets. However, high pressures (200 MPa) showed a negative effect on vitamin A retention. These results could be useful for future formulations of retinoids.
The Fractional Derivative of the Dirac Delta Function and Additional Results on the Inverse Laplace Transform of Irrational Functions
Authors: Makris Nicos
Published: February 2021

Motivated from studies on anomalous relaxation and diffusion, we show that the memory function M(t) of complex materials, that their creep compliance follows a power law, J(t)∼tq with q∈R+, is proportional to the fractional derivative of the Dirac delta function, dqδ(t−0)dtq with q∈R+. This leads to the finding that the inverse Laplace transform of sq for any q∈R+ is the fractional derivative of the Dirac delta function, dqδ(t−0)dtq. This result, in association with the convolution theorem, makes possible the calculation of the inverse Laplace transform of sqsα∓λ where α<q∈R+, which is the fractional derivative of order q of the Rabotnov function εα−1(±λ,t)=tα−1Eα,α(±λtα). The fractional derivative of order q∈R+ of the Rabotnov function, εα−1(±λ,t) produces singularities that are extracted with a finite number of fractional derivatives of the Dirac delta function depending on the strength of q in association with the recurrence formula of the two-parameter Mittag–Leffler function.
Black Holes and Other Clues to the Quantum Structure of Gravity
Authors: Giddings Steven B.
Published: February 2021

Bringing gravity into a quantum-mechanical framework is likely the most profound remaining problem in fundamental physics. The “unitarity crisis” for black hole evolution appears to be a key facet of this problem, whose resolution will provide important clues. Investigating this raises the important structural question of how to think about subsystems and localization of information in quantum gravity. Paralleling field theory, the answer to this is expected to be an important ingredient in the mathematical structure of the theory. Perturbative gravity results indicate a structure different from that of quantum field theory, but suggest an avenue to defining subsystems. If black holes do behave similarly to familiar subsystems, unitarity demands new interactions that transfer entanglement from them. Such interactions can be parameterized in an effective approach, without directly addressing the question of the fundamental dynamics, whether that is associated with quantum spacetime, wormholes, or something else. Since such interactions need to extend outside the horizon, that raises the question of whether they can be constrained, or might be observed, by new electromagnetic or gravitational wave observations of strong gravity regions. This note overviews and provides connections between these developments.
Recently Developed Carbohydrate Based Gelators and Their Applications
Authors: Morris Joedian , Bietsch Jonathan , Bashaw Kristen , Wang Guijun
Published: February 2021

Carbohydrate based low molecular weight gelators have been an intense subject of study over the past decade. The self-assembling systems built from natural products have high significance as biocompatible materials and renewable resources. The versatile structures available from naturally existing monosaccharides have enriched the molecular libraries that can be used for the construction of gelators. The bottom-up strategy in designing low molecular weight gelators (LMWGs) for a variety of applications has been adopted by many researchers. Rational design, along with some serendipitous discoveries, has resulted in multiple classes of molecular gelators. This review covers the literature from 2017–2020 on monosaccharide based gelators, including common hexoses, pentoses, along with some disaccharides and their derivatives. The structure-based design and structure to gelation property relationships are reviewed first, followed by stimuli-responsive gelators. The last section focuses on the applications of the sugar based gelators, including their utilization in environmental remediation, ion sensing, catalysis, drug delivery and 3D-printing. We will also review the available LMWGs and their structure correlations to the desired properties for different applications. This review aims at elucidating the design principles and structural features that are pertinent to various applications and hope to provide certain guidelines for researchers that are working at the interface of chemistry, biochemistry, and materials science.
Optic Atrophy and Inner Retinal Thinning in CACNA1F-Related Congenital Stationary Night Blindness
Authors: Leahy Kate E , Wright Tom , Grudzinska Pechhacker Monika K , Audo Isabelle , Tumber Anupreet , Tavares Erika , MacDonald Heather , Locke Jeff , VandenHoven Cynthia , Zeitz Christina , Heon Elise , Buncic J Raymond , Vincent Ajoy
Published: February 2021

Hemizygous pathogenic variants in CACNA1F lead to defective signal transmission from retinal photoreceptors to bipolar cells and cause incomplete congenital stationary night blindness in humans. Although the primary defect is at the terminal end of first-order neurons (photoreceptors), there is limited knowledge of higher-order neuronal changes (inner retinal) in this disorder. This study aimed to investigate inner retinal changes in CACNA1F-retinopathy by analyzing macular ganglion cell layer-inner plexiform layer (GCL-IPL) thickness and optic disc pallor in 22 subjects with molecularly confirmed CACNA1F-retinopathy. Detailed ocular phenotypic data including distance and color vision, refraction and electroretinogram (ERG) were collected. Distance vision was universally reduced (mean: 0.42 LogMAR), six had abnormal color vision and myopia was common (n = 15; mean: −6.32 diopters). Mean GCL-IPL thickness was significantly lower in patients (55.00 µm) compared to age-matched controls (n = 87; 84.57 µm; p << 0.001). The GCL-IPL thickness correlated with scotopic standard (p = 0.04) and bright-flash (p = 0.014) ERG b/a ratios and photopic b-wave amplitudes (p = 0.05). Twenty-one patients had some degree of disc pallor (bilateral in 19). Fifteen putative disease-causing, including five novel variants were identified. This study establishes macular inner retinal thinning and optic atrophy as characteristic features of CACNA1F-retinopathy, which are independent of myopia and could impact potential future treatment strategies.
Application of Genomics to Understand Salt Tolerance in Lentil
Authors: Dissanayake Ruwani , Cogan Noel O.I. , Smith Kevin F. , Kaur Sukhjiwan
Published: February 2021

Soil salinity is a major abiotic stress, limiting lentil productivity worldwide. Understanding the genetic basis of salt tolerance is vital to develop tolerant varieties. A diversity panel consisting of 276 lentil accessions was screened in a previous study through traditional and image-based approaches to quantify growth under salt stress. Genotyping was performed using two contrasting methods, targeted (tGBS) and transcriptome (GBS-t) genotyping-by-sequencing, to evaluate the most appropriate methodology. tGBS revealed the highest number of single-base variants (SNPs) (c. 56,349), and markers were more evenly distributed across the genome compared to GBS-t. A genome-wide association study (GWAS) was conducted using a mixed linear model. Significant marker-trait associations were observed on Chromosome 2 as well as Chromosome 4, and a range of candidate genes was identified from the reference genome, the most plausible being potassium transporters, which are known to be involved in salt tolerance in related species. Detailed mineral composition performed on salt-treated and control plant tissues revealed the salt tolerance mechanism in lentil, in which tolerant accessions do not transport Na+ ions around the plant instead localize within the root tissues. The pedigree analysis identified two parental accessions that could have been the key sources of tolerance in this dataset.
Identification and Characterization of the APX Gene Family and Its Expression Pattern under Phytohormone Treatment and Abiotic Stress in Populus trichocarpa
Authors: Leng Xue , Wang Hanzeng , Zhang Shuang , Qu Chunpu , Yang Chuanping , Xu Zhiru , Liu Guanjun
Published: February 2021

Ascorbate peroxidase (APX) is a member of class I of the heme-containing peroxidase family. The enzyme plays important roles in scavenging reactive oxygen species for protection against oxidative damage and maintaining normal plant growth and development, as well as in biotic stress responses. In this study, we identified 11 APX genes in the Populus trichocarpa genome using bioinformatic methods. Phylogenetic analysis revealed that the PtrAPX proteins were classifiable into three clades and the members of each clade shared similar gene structures and motifs. The PtrAPX genes were distributed on six chromosomes and four segmental-duplicated gene pairs were identified. Promoter cis-elements analysis showed that the majority of PtrAPX genes contained a variety of phytohormone- and abiotic stress-related cis-elements. Tissue-specific expression profiles indicated that the PtrAPX genes primarily function in roots and leaves. Real-time quantitative PCR (RT-qPCR) analysis indicated that PtrAPX transcription was induced in response to drought, salinity, high ammonium concentration, and exogenous abscisic acid treatment. These results provide important information on the phylogenetic relationships and functions of the APX gene family in P. trichocarpa.
Comparative Transcriptome Analysis Reveals Key Genes and Pathways Involved in Prickle Development in Eggplant
Authors: Zhang Lei , Sun Haoyun , Xu Tao , Shi Tianye , Li Zongyun , Hou Wenqian
Published: February 2021

Eggplant is one of the most important vegetables worldwide. Prickles on the leaves, stems and fruit calyxes of eggplant may cause difficulties during cultivation, harvesting and transportation, and therefore is an undesirable agronomic trait. However, limited knowledge about molecular mechanisms of prickle morphogenesis has hindered the genetic improvement of eggplant. In this study, we performed the phenotypic characterization and transcriptome analysis on prickly and prickleless eggplant genotypes to understand prickle development at the morphological and molecular levels. Morphological analysis revealed that eggplant prickles were multicellular, lignified and layered organs. Comparative transcriptome analysis identified key pathways and hub genes involved in the cell cycle as well as flavonoid biosynthetic, photosynthetic, and hormone metabolic processes during prickle development. Interestingly, genes associated with flavonoid biosynthesis were up-regulated in developing prickles, and genes associated with photosynthesis were down-regulated in developing and matured prickles. It was also noteworthy that several development-related transcription factors such as bHLH, C2H2, MYB, TCP and WRKY were specifically down- or up-regulated in developing prickles. Furthermore, four genes were found to be differentially expressed within the Pl locus interval. This study provides new insights into the regulatory molecular mechanisms underlying prickle morphogenesis in eggplant, and the genes identified might be exploited in breeding programs to develop prickleless eggplant cultivars.
Neuroacanthocytosis Syndromes in an Italian Cohort: Clinical Spectrum, High Genetic Variability and Muscle Involvement
Authors: Vaisfeld Alessandro , Bruno Giorgia , Petracca Martina , Bentivoglio Anna Rita , Servidei Serenella , Vita Maria Gabriella , Bove Francesco , Straccia Giulia , Dato Clemente , Di Iorio Giuseppe , Sampaolo Simone , Peluso Silvio , De Rosa Anna , De Michele Giuseppe , Barghigiani Melissa , Galatolo Daniele , Tessa Alessandra , Santorelli Filippo , Chiurazzi Pietro , Melone Mariarosa Anna Beatrice
Published: February 2021

Neuroacanthocytosis (NA) syndromes are a group of genetically defined diseases characterized by the association of red blood cell acanthocytosis, progressive degeneration of the basal ganglia and neuromuscular features with characteristic persistent hyperCKemia. The main NA syndromes include autosomal recessive chorea-acanthocytosis (ChAc) and X-linked McLeod syndrome (MLS). A series of Italian patients selected through a multicenter study for these specific neurological phenotypes underwent DNA sequencing of the VPS13A and XK genes to search for causative mutations. Where it has been possible, muscle biopsies were obtained and thoroughly investigated with histochemical assays. A total of nine patients from five different families were diagnosed with ChAC and had mostly biallelic changes in the VPS13A gene (three nonsense, two frameshift, three splicing), while three patients from a single X-linked family were diagnosed with McLeod syndrome and had a deletion in the XK gene. Despite a very low incidence (only one thousand cases of ChAc and a few hundred MLS cases reported worldwide), none of the 8 VPS13A variants identified in our patients is shared by two families, suggesting the high genetic variability of ChAc in the Italian population. In our series, in line with epidemiological data, McLeod syndrome occurs less frequently than ChAc, although it can be easily suspected because of its X-linked mode of inheritance. Finally, histochemical studies strongly suggest that muscle pathology is not simply secondary to the axonal neuropathy, frequently seen in these patients, but primary myopathic alterations can be detected in both NA syndromes.
The Regulation of RNA Modification Systems: The Next Frontier in Epitranscriptomics?
Authors: Schaefer Matthias R.
Published: February 2021

RNA modifications, long considered to be molecular curiosities embellishing just abundant and non-coding RNAs, have now moved into the focus of both academic and applied research. Dedicated research efforts (epitranscriptomics) aim at deciphering the underlying principles by determining RNA modification landscapes and investigating the molecular mechanisms that establish, interpret and modulate the information potential of RNA beyond the combination of four canonical nucleotides. This has resulted in mapping various epitranscriptomes at high resolution and in cataloguing the effects caused by aberrant RNA modification circuitry. While the scope of the obtained insights has been complex and exciting, most of current epitranscriptomics appears to be stuck in the process of producing data, with very few efforts to disentangle cause from consequence when studying a specific RNA modification system. This article discusses various knowledge gaps in this field with the aim to raise one specific question: how are the enzymes regulated that dynamically install and modify RNA modifications? Furthermore, various technologies will be highlighted whose development and use might allow identifying specific and context-dependent regulators of epitranscriptomic mechanisms. Given the complexity of individual epitranscriptomes, determining their regulatory principles will become crucially important, especially when aiming at modifying specific aspects of an epitranscriptome both for experimental and, potentially, therapeutic purposes.
Image, Environment, Infrastructure: The Social Ecologies of the Bergfilm
Authors: Peabody Seth
Published: February 2021

The German mountain film (Bergfilm) has received extensive critical attention for its political, social, and aesthetic implications, but has received remarkably little attention for its role in the environmental history of the Alps. This article considers the Bergfilm within the long history of depictions of the Alps and the growth of Alpine tourism in order to ask how the role of media in environmental change shifts with the advent of film. The argument builds on Verena Winiwarter and Martin Knoll’s model of social-ecological interaction, Adrian Ivakhiv’s theoretical framework for the environmental implications of film, and Laura Frahm’s theories of filmic space. Through an analysis of Arnold Fanck’s films Der heilige Berg [The Holy Mountain, Fanck 1926] and Der große Sprung [The Great Leap, Fanck 1927], which are compared with Gustav Renker’s novel Heilige Berge [Holy Mountains, Renker 1921] and set into the context of the environmental history of the Alpine regions where the films were shot, the author argues that film aesthetics serve as a creative catalyst for environmental change and infrastructure development. While some ecocinema scholars have argued that environmental films teach viewers new ideas or change modes of behavior, this analysis suggests that film aesthetics are most effective at accelerating processes of environmental change that are already underway.
Yellow Lake Pigments from Weld in Art: Investigating the Winsor & Newton 19th Century Archive
Authors: Veneno Maria , Nabais Paula , Otero Vanessa , Clemente Adelaide , Oliveira M. Conceição , Melo Maria João
Published: February 2021

Weld (Reseda luteola) was one of the main sources of yellow dyes used for dyeing textiles and to prepare artists’ pigments in Europe until the 19th century. For the first time, this work explores the technology of preparing weld lake pigments in the 19th century by Winsor & Newton (W&N), a renowned supplier of artists’ materials. Five recipes were discovered in the W&N 19th century Archive Database and reconstructed in the laboratory. W&N was extracting weld in neutral and basic media, and preparing the insoluble lake by complexation with Al3+ in the form of alum (KAl(SO4)2•12H2O) or hydrated alumina (Al(OH)3). Five yellow lake pigments were successfully obtained and characterized by High-Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) and Fourier Transform Infrared Spectroscopy (FTIR). Their chromatographic profiles display as main yellows, luteolin 7-O-glucoside (Lut-7-O-glu) or both Lut-7-O-glu plus luteolin 3′,7-O-glucoside (Lut-3’,7-O-glu). In two of the processes, the presence of gypsum (CaSO4•2H2O) was unequivocally detected by FTIR, being formed as a by-product. This work offers the first identification of weld lake pigments’ characteristic infrared bands. The W&N Database proved again to be a unique source of information on 19th-century artists’ materials and their commercial preparation. The knowledge gain is essential to ensure effective conservation and authentication procedures.
Food Safety and E. coli in Aquaponic and Hydroponic Systems
Authors: Sawyer Tawnya
Published: February 2021

The following document is The Aquaponic Association’s response to a recent publication on E. coli in Aquaponic and Hydroponic systems [...]
An Assessment of Treated Greywater Reuse in Irrigation on Growth and Protein Content of Prosopis and Albizia
Authors: Al-Mefleh Naji K. , Othman Yahia A. , Tadros Maher J. , Al-Assaf Amani , Talozi Samer
Published: February 2021

This study investigated the influence of treated greywater on growth and protein content of multipurpose (forage and ornamental) transplants, Prosopis juliflora L., Prosopis tamarugo L., and Albizia lebbeck L. Transplants of tested species were irrigated with treated greywater, diluted greywater (grey + distilled water, 1:1/by volume), and distilled water (control) for seven months. Water quality analysis showed that the concentrations of nutrients and heavy metals found in the greywater were within the acceptable range compared with Jordan Institution for Standard and Metrology (JISM) and the World Health Organization (WHO) thresholds for safe use of greywater. Escherichia coli found in the greywater were lower compared to JISM and WHO guidelines for the safe use of greywater. Irrigation with treated greywater increased shoot fresh weight by 24–39% and dry weight by 34–40% compared to diluted greywater and control. No significant difference in crude protein was noticed between water treatments. Prosopis species (P. juliflora Albizia lebbeck L. and P. tamarugo Albizia lebbeck L.) had higher shoot fresh (35%) and dry weight the same species had lower crude protein (44%) when compared to Albizia lebbeck Albizia lebbeck L. The reuse of treated greywater for landscaping or forage production alleviates the demand for water resources and reduces the pressure on wastewater treatment plants. However, considering the controversial findings of previous studies on greywater quality (especially, long-term reuse), the reuse of treated greywater needs to be considered with caution and periodic quality analyses and economic assessments are required.
Residence Time Analysis in the Albufera of Valencia, a Mediterranean Coastal Lagoon, Spain
Authors: Soria Juan , Vera-Herrera Lucía , Calvo Sara , Romo Susana , Vicente Eduardo , Sahuquillo María , Sòria-Perpinyà Xavier
Published: February 2021

The Albufera of Valencia is a coastal lagoon located in the western area of the Mediterranean Sea, in the Iberian Peninsula. It has an area of 23.1 km2 and an average depth of only 1 m, with a maximum depth of 1.6 m. This lagoon is the remnants of an original and more extensive wetland of about 220 km2 which is now mostly dedicated to rice cultivation. Surface water is supplied through several main and many secondary canals for a total of 64 water entry points and three exit points to the sea. It is difficult to evaluate the residence time due to the lack of reliable measurements of the inflow or outflow, as well as continuous measurements. Between 1988 and 2018, several procedures were used, the results of which are outlined in this document. Overall, a decrease in the inflow during these thirty years was observed and, therefore, it can be concluded that the residence time is increasing. There is a temporal variation during the year due to rainfall and cultivation periods. Likewise, the results found that the natural hydrological zoning of the lagoon causes a spatial heterogeneity with small Northern areas with low residence time of 4.7 days, almost on a weekly basis and large Western extensions with high residence time of 222.9 days. It is impossible to know this information if individual flow measurements are not taken from each of the main watercourses.
Risk Assessment of Future Climate and Land Use/Land Cover Change Impacts on Water Resources
Authors: Martin Nick
Published: February 2021

Climate and land use and land cover (LULC) changes will impact watershed-scale water resources. These systemic alterations will have interacting influences on water availability. A probabilistic risk assessment (PRA) framework for water resource impact analysis from future systemic change is described and implemented to examine combined climate and LULC change impacts from 2011–2100 for a study site in west-central Texas. Internally, the PRA framework provides probabilistic simulation of reference and future conditions using weather generator and water balance models in series—one weather generator and water balance model for reference and one of each for future conditions. To quantify future conditions uncertainty, framework results are the magnitude of change in water availability, from the comparison of simulated reference and future conditions, and likelihoods for each change. Inherent advantages of the framework formulation for analyzing future risk are the explicit incorporation of reference conditions to avoid additional scenario-based analysis of reference conditions and climate change emissions scenarios. In the case study application, an increase in impervious area from economic development is the LULC change; it generates a 1.1 times increase in average water availability, relative to future climate trends, from increased runoff and decreased transpiration.
Total Hip Arthroplasty: Minimal Clinically Important Difference and Patient Acceptable Symptom State for the Forgotten Joint Score 12
Authors: Longo Umile Giuseppe , De Salvatore Sergio , Piergentili Ilaria , Indiveri Anna , Di Naro Calogero , Santamaria Giulia , Marchetti Anna , Marinis Maria Grazia De , Denaro Vincenzo
Published: February 2021

The Forgotten Joint Score-12 (FJS-12) is a valid patient-reported outcome measures (PROMs) used to assess prosthesis awareness during daily activities after total hip arthroplasty (THA). The minimum clinically important difference (MCID) can be defined as the smallest change or difference that is evaluated as beneficial and could change the patient’s clinical management. The patient acceptable symptom state (PASS) is considered the minimum PROMs cut-off value that corresponds to a patient’s satisfactory state of health. Despite the validity and reliability of the FJS-12 having been already demonstrated, the MCID and the PASS of this score have not previously been defined. Patients undergoing THA from January 2019 to October 2019 were assessed pre-operatively and six months post-surgery using the FJS-12, the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) and the Oxford Hip Score (OHS). Pre-operative and follow-up questionnaires were completed by 50 patients. Both distribution-based approaches and anchor approaches were used to estimate MCID. The aim of this paper was to assess the MCID and PASS values of FJS-12 after total hip replacement. The FJS-12 MCID from baseline to 6 months post-operative follow-up was 17.5. The PASS calculated ranged from 69.8 to 91.7.
Cost-Free LTC Model Incorporated into Private Pension Schemes
Authors: De La Peña J. Iñaki , Fernández-Ramos M. Cristina , Garayeta Asier
Published: February 2021

Long-term care coverage is not integrated into an individual’s retirement strategy. It is an additional public health service that is not considered into private pension funds. Nevertheless, this coverage is not sufficient due to the problems of financial sustainability of the public pension systems. However, there are large sums in pension plans dedicated to paying retirement pensions that can be transformed into support for long-term care coverage. This paper develops a mechanism of pension transformation through the different mortality of the beneficiary when becoming a dependent beneficiary. This mechanism allows the beneficiary to convert their pension to LTC support at their own choice, without increasing the cost of the private pension scheme. The proposed model provides consistency in the pension that a retiree receives and adapts it to a retiree’s life expectancy: the retiree receives a higher pension when he/she needs it most.