bluuebunny/crossref_metadata_2025 huggingface dataset:
Rosemary Extract and Essential Oil as Drink Ingredients: An Evaluation of Their Chemical Composition, Genotoxicity, Antimicrobial, Antiviral, and Antioxidant Properties
Authors: Christopoulou Spyridoula D. , Androutsopoulou Chrysa , Hahalis Panagiotis , Kotsalou Chrysoula , Vantarakis Apostolos , Lamari Fotini N.
Published: December 2021

Rosmarinus officinalis L. (rosemary) is in high demand in the food and drink industries due to its distinct organoleptic properties. With the aim of evaluating the rosemary leaves as drink ingredients, both the essential oil and alcoholic (38%, v/v) extract were studied in terms of chemical composition, genotoxicity, antimicrobial, antiviral, and antioxidant properties. GC–MS analysis showed that the main volatile compounds in the essential oil were eucalyptol (40.1%), camphor (12.4%), and α-pinene (12.9%). LC–MS analysis revealed gallocatechin and rosmarinic acid as the main extract ingredients. Both the essential oil and the extract were not genotoxic (Ames test) against TA98 and TA100 at the dilutions of 5% and 90%, respectively; those dilutions were selected as the maximum possible ones in the drink industry. Their activity was investigated against Escherichia coli, Salmonella enterica serovar Typhimurium, Staphylococcus aureus, Aspergillus niger, and Adenovirus 35. Both were effective against Adenovirus and A. niger, even the essential oil at 5% (v/v). The extract at dilutions of 25–90% had more pronounced activity against tested bacteria than the essential oil at the dilutions of 5–100%; the essential oil at the dilution of 5% inhibited S. aureus growth. The antioxidant activity was evaluated by the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, the 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid decolorization assay, and the ferric reducing antioxidant power assay. Both exhibited good antioxidant activity, but rosemary essential oil was far more effective than the extract. Our results demonstrate that rosemary essential oil and extract are safe and have beneficial biological properties. Therefore, they could serve as health-promoting ingredients in the drink industry.
Social Media Analysis to Understand the Expected Benefits by Plant-Based Meat Alternatives Consumers
Authors: Moreira Mirian Natali Blézins , da Veiga Cássia Rita Pereira , Su Zhaohui , Reis Germano Glufke , Pascuci Lucilaine Maria , da Veiga Claudimar Pereira
Published: December 2021

The plant-based alternative meat products market has attracted attention in recent years, as the demand for these products has grown worldwide. To meet the needs of this promising market, marketers must pay attention to the expected benefits of consumers and the insights that can be gleaned from comments posted on social media. This article proposed an investigation of the potential of the content analysis of comments posted on the Instagram social network of food companies that manufacture plant-based alternative meat products to understand the expected benefits by end consumers from the perspective of the classic marketing mix variables. The content posted voluntarily by consumers was organized into 13 categories of expected benefits analyzed within a proposal of evidence from the perspective of the marketing mix. The results showed that, among the insights obtained, 63% were related to the place variable, 21% to the product variable, 11% to the price variable, and 5% to the promotion variable. The insights reinforce the notion that marketing mix variables are crucial factors for companies to make products available in the right place, in the right quantity, and at a fair price, in addition to engaging with consumers through social media.
Procyanidins: From Agro-Industrial Waste to Food as Bioactive Molecules
Authors: Valencia-Hernandez Leidy Johana , Wong-Paz Jorge E. , Ascacio-Valdés Juan Alberto , Chávez-González Mónica L. , Contreras-Esquivel Juan Carlos , Aguilar Cristóbal N.
Published: December 2021

Procyanidins are an important group of bioactive molecules known for their benefits to human health. These compounds are promising in the treatment of chronic metabolic diseases such as cancer, diabetes, and cardiovascular disease, as they prevent cell damage related to oxidative stress. It is necessary to study effective extraction methods for the recovery of these components. In this review, advances in the recovery of procyanidins from agro-industrial wastes are presented, which are obtained through ultrasound-assisted extraction, microwave-assisted extraction, supercritical fluid extraction, pressurized fluid extraction and subcritical water extraction. Current trends focus on the extraction of procyanidins from seeds, peels, pomaces, leaves and bark in agro-industrial wastes, which are extracted by ultrasound. Some techniques have been coupled with environmentally friendly techniques. There are few studies focused on the extraction and evaluation of biological activities of procyanidins. The identification and quantification of these compounds are the result of the study of the polyphenolic profile of plant sources. Antioxidant, antibiotic, and anti-inflammatory activity are presented as the biological properties of greatest interest. Agro-industrial wastes can be an economical and easily accessible source for the extraction of procyanidins.
The Photometric and Spectroscopic Properties of Remnant and Restarted Radio Galaxies in the Lockman Hole Field
Authors: Jurlin Nika , Morganti Raffaella , Maddox Natasha , Brienza Marisa
Published: December 2021

Radio galaxies are known to undergo phases of activity, where the stage after the jets have switched off is referred to as the remnant phase. This state can be followed by a restarted phase when the activity reignites. Remnant and restarted radio sources are important for testing models of the evolution of radio active galactic nuclei (AGN) and for understanding the impact the recurrent jet activity has on their host galaxies. Although we now have statistical samples of radio sources in various stages of their life cycle, how this intermittent radio activity is reflected in the optical properties in this sample has not yet been addressed, and is overall a much less studied aspect in the literature. In this work, we use the Wide-field Infrared Survey Explorer and the Sloan Digital Sky Survey (SDSS) photometry, and SDSS spectra to study these properties in a sample of the remnant, candidate restarted, and active radio galaxies selected using the LOw Frequency ARray at 150 MHz in the Lockman Hole extragalactic field. Within the range of radio luminosities and stellar masses studied in this work, we find no difference between the properties of the host galaxy and of the optical emission lines for objects in different phases of their radio life cycle. The vast majority of our radio sources (either remnant, candidate restarted, or comparison sample) are associated with radiatively inefficient optical AGN and red galaxies dominated by old stellar populations. Thus, the radio and emission-line AGN activity appears to be independent and regulated by different mechanisms. This suggests that, at least for the radio luminosities of our sample, the life cycle of the radio may depend on intrinsic reasons, such as the stability of the accretion disc, more than variation in the accretion rate and fuelling of the central black hole.
Review of Solutions to the Cusp-Core Problem of the ΛCDM Model
Authors: Del Popolo Antonino , Le Delliou Morgan
Published: December 2021

This review aims at proposing to the field an overview of the Cusp-core problem, including a discussion of its advocated solutions, assessing how each can satisfactorily provide a description of central densities. Whether the Cusp-core problem reflects our insufficient grasp on the nature of dark matter, of gravity, on the impact of baryonic interactions with dark matter at those scales, as included in semi-analytical models or fully numerical codes, the solutions to it can point either to the need for a paradigm change in cosmology, or to to our lack of success in ironing out the finer details of the ΛCDM paradigm.
Novel Thermosensitive-co-Zwitterionic Sulfobetaine Gels for Metal Ion Removal: Synthesis and Characterization
Authors: Ningrum Eva Oktavia , Gotoh Takehiko , Ciptonugroho Wirawan , Karisma Achmad Dwitama , Agustiani Elly , Safitri Zela Marni , Dzaky Muhammad Asyam
Published: December 2021

Zwitterionic betaine polymers are promising adsorbents for the removal of heavy metal ions from industrial effluents. Although the presence of both negative and positively charged groups imparts them the ability to simultaneously remove cations and anions, intra- and/or inter-chain interactions can significantly reduce their adsorption efficiencies. Therefore, in this study, novel gels based on crosslinked co-polymers of thermosensitive N-isopropylacrylamide (NIPAAM) and zwitterionic sulfobetaine N,N-dimethylacrylamido propyl ammonium propane sulfonate (DMAAPS) were synthesized, characterized, and evaluated for ion removal. Fourier-transform infrared (FTIR) and proton nuclear magnetic resonance (1H NMR) analyses confirmed the success of the co-polymerization of NIPAAM and DMAAPS to form poly(NIPAAM-co-DMAAPS). The phase transition temperature of the co-polymer increased with increasing DMAAPS content in the co-polymer, indicating temperature-dependent amphiphilic behavior, as evidenced by contact angle measurements. The ion adsorption analyses of the poly(NIPAAM-co-DMAAPS) gels indicated that co-polymerization increased the molecular distance and weakened the interaction between the DMAAPS-charged groups (SO3− and N+), thereby increasing the ion adsorption. The results confirmed that, with a low concentration of DMAAPS in the co-polymer gels (~10%), the maximum amount of Cr3+ ions adsorbed onto the gel was ~58.49% of the sulfonate content in the gel.
Qualitative-Quantitative Assessment of Geodiversity of Western Samoa (SW Pacific) to Identify Places of Interest for Further Geoconservation, Geoeducation, and Geotourism Development
Authors: Zakharovskyi Vladyslav , Németh Károly
Published: December 2021

The assessment of geodiversity is a relatively new field of research connecting abiotic aspects of nature to the wider environment. The study of geodiversity is still in development, so a uniform and complete paradigm remains to be defined. Therefore, an assessment of geodiversity may be highly dependent on the nature of the territory subject to study, available databases, and the researchers’ field of specialization. The main quantitative method for the assessment of geodiversity was proposed to the scientific world only few years ago and may only be relevant to some places in the world, rather than all, which would be desirable. However, while similarities in research methods may be apparent, the directions, scales, and data utilized are clearly different. This article demonstrates a quantitative-qualitative method for an assessment of geodiversity, based on a five-point evaluation system and the utilization of widely available standard databases such as geological maps, SRTM models, and satellite images. Western Samoa Islands (Savai’i and Upolu Islands) were selected for assessment, as a typical example of basaltic ocean island volcanism generating relatively homogenous rock formations and subject to gradual geomorphology (e.g., shield volcano). While initially appearing as a region of simple geology and morphology, complexity is added by considering rock ages, the position and type of eruptive centres, and the coastal geoenvironment. By considering these factors, the assessment becomes specifically tailoring for geodiversity assessment of the islands of Samoa. In conclusion, it has been demonstrated a simple methodology of general assessment of geodiversity with additional improvements to take account of variability in other abiotic factors.
Is the Severity of Cervical Foraminal Stenosis Related to the Severity and Sidedness of Symptoms?
Authors: Lee Han-Dong , Jeon Chang-Hoon , Chung Nam-Su , Yoon Ha-Seung , Chung Hee-Woong
Published: December 2021

(1) Background: Cervical foraminal stenosis (CFS) is a common cause of axial neck and arm pain. The aim of this study was to determine the relationship between the severity of CFS and clinical symptoms in terms of severity and sidedness. (2) Methods: We retrospectively reviewed 75 consecutive patients with degenerative CFS. We graded 900 foramina from C3–4 to T1–2 using Park’s grading system. We collected visual analogue scale (VAS) and neck disability index (NDI) values from the neck and both arms. We analyzed the relationships with CFS grades and total number. We defined four types of left/right dominance of CFS (none, left-dominant, right-dominant, and both) by comparing left and right sides using total counts and maximal grade of CFS, respectively. We compared arm pain sidedness (no arm pain, left-only, right only, and bilateral) among different left and right CFS dominance types. (3) Results: Mean neck and left and right arm VAS scores were 4.4 ± 2.5, 4.9 ± 1.6, and 4.6 ± 2.0, respectively. The mean total NDI was 16.0 ± 8.0. The CFS grade at C3–4 and total count were correlated with neck VAS. Arm VAS was also correlated with CFS grade and total counts. Total NDI score was not correlated with radiological parameters. The presence and sidedness of arm pain were significantly different between left and right CFS dominance groups divided by total count of grade 2 and 3 CFS. (4) Conclusions: The CFS grade and total count were correlated with neck and arm VAS. Arm pain occurred more frequently on the side with more grade 2 and 3 CFS.
Physicians’ Experience with COVID-19 Vaccination: A Survey Study
Authors: Dima Alina , Jurcut Ciprian , Balaban Daniel Vasile , Gheorghita Valeriu , Jurcut Ruxandra , Dima Augustin Catalin , Jinga Mariana
Published: December 2021

Background: COVID-19 vaccine hesitancy remains high in the general population and is the main determinant of low vaccination rates and of the fourth pandemic wave severity in Romania. Additional information is needed to raise awareness over vaccine efficiency and the safety profile. Objective: To assess self-reported experience related to COVID-19 vaccination in Romanian physicians. Design, Setting, and Participants: A cross-sectional, self-administered questionnaire-based survey, distributed online in the period 24 March to 24 May 2021. The survey included 30 cascade questions with skip logic filters. All physicians included filled in the questionnaire voluntarily and anonymously. Not all respondents filled in all questions. Main outcome and measure: Primary outcomes addressed were related to the COVID-19 vaccine safety profile. Results: 407/467 (87.15%) of the respondents’ physicians were fully vaccinated, mostly with the Pfizer—BioNTech (Comirnaty)—BNT162b2 vaccine, with the peak of immunization in January 2021, with almost four-fifths of the study participants. Regarding COVID-19, almost 20% physicians had the infection and one declared COVID-19 re-infection. A number of 48/420 (11.42%) and 47/419 (11.22%) of the vaccinated physicians did not report any side effects after the first or second vaccine dose. However, most of the side effects reported were minor. Only 50/360 (13.88%) physicians reported the vaccine side effects on the dedicated online national platform. Approximately 40% respondents checked the anti-spike SARS-CoV2 antibodies’ titer after complete vaccination, of which two cases reported indeterminate levels. Lower anti-spike SARS-CoV2 antibodies’ titer of 100–1000 times the laboratory limit was more frequent in naive physicians (36.95% versus 14.28%, p = 0.012), moderate titers were similar, while very high levels, more than 10,000 times laboratory limit, were more frequent in physicians with previous COVID-19 infection (2.17% versus 42.85%, p < 0.001). Conclusions and relevance: In this cross-sectional survey study on the COVID-19 vaccination among Romanian physicians, we describe a safety vaccination profile among Romanian physicians.
Correlation between the Mandibular Lingula Position and Some Anatomical Landmarks in Cone Beam CT
Authors: Lupi Saturnino Marco , Landini Jessica , Olivieri Giorgia , Todaro Claudia , Scribante Andrea , Rodriguez y Baena Ruggero
Published: December 2021

Background: the position of the mandibular lingula (Li) affects the success rate of the inferior alveolar nerve block (IANB) and ramus osteotomies. This study evaluated the position of the Li, to investigate the anatomical relationship between the Li and some anatomical measurements using cone beam computed tomography (CBCT). Methods: 201 hemimandibular CBCTs of 111 patients (43 males and 68 females; 18 to 88 years old) were retrospectively evaluated. The Li location was determined from the lingula tip to: the occlusal plane, the anterior and posterior borders of the mandibular ramus, the lower border of the mandible, the distal surface of the mandibular second molar, and the mandibular notch. We evaluated the correlations between the Li and the anteroposterior diameter of the mandibular ramus; the vertical distance between condyle and mandibular angle; the mesial–distal diameter of the first, second, and third mandibular molar, the intercanine distance, the intermolar distances among the first, second, and third mandibular molars; the distance between the intermolar line of the first molar and midline, and the length of the mandibular body. Results: the vertical distance of the Li from the occlusal plane was 11.22 ± 4.27 mm. Some parameters significantly correlated with the anatomical measurements taken into consideration. Conclusions: the present study provides new information concerning the Li and mandibular anatomy in the Italian population. Moreover, by correlating some anatomic measurements to the Li position, the localization of the Li is made possible, indirectly through the measurement of some distances between anatomical landmarks.
Regional Adolescent Obesity and Overweight Status in Korea from 2018–2019: Comparison between Two Data Sources
Authors: Ryu Dong-Hee , Lee Su-Jin
Published: December 2021

Difference in the regional adolescent obesity level may cause a notable health inequality between regions since it significantly affects adulthood health status. This study examined 2018 and 2019 regional obesity and overweight status of adolescents aged 12 to 18 by comparing two cross sectional population-based data sources, the Korea Youth Risk Behavior Web-based Survey (KYRBS) and the National Student Health Examination (NSHE). Prevalence was estimated by calculating weighted percentages and 95% confidence intervals. Correlations in the relative rankings of each municipality were determined by computing Spearman correlation coefficients (rs), and prevalence discrepancies between the data sources were visualized by simple correlation graphs. The geographical distributions of adolescent obesity and overweight status showed no perfect concordances between the data sources regardless of sexes and survey years. For adolescent obesity status, there were significant difference between the least and the most obese regions and rs levels were fair to good with p-values less than 0.05, but the correlation graphs indicated body mass index (BMI) underreporting in the KYRBS. For adolescent overweight status, no significant similarities were defined between the data. These results can be used as a basis for the establishment of related policies.
Testing Kissick’s Iron Triangle—Structural Equation Modeling Analysis of a Practical Theory
Authors: Beauvais Brad , Kruse Clemens Scott , Fulton Lawrence , Brooks Matthew , Mileski Michael , Lee Kim , Ramamonjiarivelo Zo , Shanmugam Ramalingam
Published: December 2021

Background/Purpose: The purpose of this research is to determine if the tradeoffs that Kissick proposed among cost containment, quality, and access remain as rigidly interconnected as originally conceived in the contemporary health care context. Although many have relied on the Kissick model to advocate for health policy decisions, to our knowledge the model has never been empirically tested. Some have called for policy makers to come to terms with the premise of the Kissick model tradeoffs, while others have questioned the model, given the proliferation of quality-enhancing initiatives, automation, and information technology in the health care industry. One wonders whether these evolutionary changes alter or disrupt the originality of the Kissick paradigms themselves. Methods: Structural equation modeling (SEM) was used to evaluate the Kissick hypothetical relationships among the unobserved constructs of cost, quality, and access in hospitals for the year 2018. Hospital data were obtained from Definitive Healthcare, a subscription site that contains Medicare data as well as non-Medicare data for networks, hospitals, and clinics (final n = 2766). Results: Reporting significant net effects as defined by our chosen study variables, we find that as quality increases, costs increase, as access increases, quality increases, and as access increases, costs increase. Policy and Practice Implications: Our findings lend continued relevance to a balanced approach to health care policy reform efforts. Simultaneously bending the health care cost curve, increasing access to care, and advancing quality of care is as challenging now as it was when the Kissick model was originally conceived.
Real-Time Tracking of Human Neck Postures and Movements
Authors: Kumar Korupalli V. Rajesh , Elias Susan
Published: December 2021

Improper neck postures and movements are the major causes of human neck-related musculoskeletal disorders. To monitor, quantify, analyze, and detect the movements, remote and non-invasive based methods are being developed for prevention and rehabilitation. The purpose of this research is to provide a digital platform for analyzing the impact of human neck movements on the neck musculoskeletal system. The secondary objective is to design a rehabilitation monitoring system that brings accountability in the treatment prescribed, which is shown in the use-case model. To record neck movements effectively, a Smart Neckband integrated with the Inertial Measurement Unit (IMU) was designed. The initial task was to find a suitable position to locate the sensors embedded in the Smart Neckband. IMU-based real-world kinematic data were captured from eight research subjects and were used to extract kinetic data from the OpenSim simulation platform. A Random Forest algorithm was trained using the kinetic data to predict the neck movements. The results obtained correlated with the novel idea proposed in this paper of using the hyoid muscles to accurately detect neck postures and movements. The innovative approach of integrating kinematic data and kinetic data for analyzing neck postures and movements has been successfully demonstrated through the efficient application in a rehabilitation use case with about 95% accuracy. This research study presents a robust digital platform for the integration of kinematic and kinetic data that has enabled the design of a context-aware neckband for the support in the treatment of neck musculoskeletal disorders.
Confinement by COVID-19 and Degree of Mental Health of a Sample of Students of Health Sciences
Authors: Rymer-Diez Arantxa , Roca-Millan Elisabet , Estrugo-Devesa Albert , González-Navarro Beatriz , López-López José
Published: December 2021

Background: In response to the global COVID-19 pandemic, most countries have taken important measures to control the spread of the virus, such as population confinement and the closure of universities. Objective: The main objective of this study is to evaluate how the situation resulting from COVID-19 has affected the mental health of a group of health sciences students. Material and Methods: This is a cross-sectional, descriptive, observational study with a sample of 160 people, conducted through an online survey of final-year students of dentistry, nursing and medicine at the Faculty of Medicine and Health Sciences of the University of Barcelona. Results: A total of 82.4% of students reported having suffered stress, anxiety and distress due to the pandemic situation, and 83.10% of participants are worried about not knowing when the academic year will restart. Conclusions: Confinement has negatively affected the mental health of students.
The Role of Music Therapy for Children Undergoing Cancer Treatment in Singapore
Authors: Wong Kayla Carissa , Tan Beron W. Z. , Tong Jasper W. K. , Chan Mei Yoke
Published: December 2021

The aim of this study was to explore the benefits of music therapy (MT) for children with cancer over the course of their treatment in an acute paediatric hospital setting in Singapore. Twenty-five children undergoing cancer treatment received MT sessions as part of a multidisciplinary team rehabilitation intervention from March 2017 to January 2020. A total of 37 individualised goals were developed by the music therapist for the cohort. Goals were scored via the Goal Attainment Scale at 3-month intervals up to 1 year. Descriptive statistics and correlation analysis were used to evaluate the findings. The rate of goal achievement was 89.2% over 180 MT sessions (M = 7.20, SD = 6.45). Children diagnosed with brain tumours had the highest frequency of MT sessions (M = 9.11, SD = 7.79). Most of the goals targeted the regulation of mood and morale through music. There was a positive correlation found between goals and sessions (rs = 0.56, p = 0.004). Age of the children was not correlated with the number of sessions received (rs= −0.19, p = 0.354). MT has been found to be an accessible and effective intervention in addressing functional and emotional goals for children across all ages who are undergoing cancer treatment.
Monitoring the Green Vegetation Period of Two Narcissus Taxa by Non-Destructive Analysis of Selected Physiological and Morphological Properties
Authors: Jezdinská Slezák Katalin , Jezdinský Aleš , Vachůn Miroslav , Sotolářová Oldřiška , Pokluda Robert , Uher Jiří
Published: December 2021

In a pot experiment, an early-flowering Narcissus pseudonarcissus cv. ‘Dutch Master’ (DM) and late-flowering N. poeticus cultural form (PO) were examined. The photosynthetic rate (A), transpiration rate (E), stomatal conductance (gs), photosynthetic water use efficiency (WUE), relative chlorophyll content (CCI) and chlorophyll fluorescence (F) were measured regularly. Leaf length, scape length and weight of the plant organs were also measured. The DM cultivar had higher gs and lower E values than the PO on most measuring dates (season average: gs: DM: 165.34, PO: 123.63; E: DM: 1.39, PO: 1.78 mmol H2O m−2s−1). The A curve was similar for the two taxa, except for the first measuring dates. The basic F values (F0, Fm, Fv) for DM were lower and CCI values were higher than for PO (season average of CCI: DM: 94.82, PO: 60.34). The Fm/F0, Fv/F0 and CCI curves were well described by a second order equation. The seasonal change of F and CCI values was the greatest for both taxa near the leaf tip. Bulb growth occurred in the two taxa in approximately the same calendar period, regardless of flowering time. A significant part of the leaf growth in DM occurred after flowering, while the leaves of PO reached their mature size by flowering.
In Vitro Propagation of Aconitum chasmanthum Stapf Ex Holmes: An Endemic and Critically Endangered Plant Species of the Western Himalaya
Authors: Rafiq Shah , Wagay Nasir Aziz , Bhat Irshad Ahmad , Kaloo Zahoor Ahmad , Rashid Sumaira , Lin Feng , El-Abedin Tarek K Zin , Wani Shabir Hussain , Mahmoud Eman A. , Almutairi Khalid F. , Elansary Hosam O.
Published: December 2021

Aconitum chasmanthum Stapf ex Holmes, a highly valued medicinal plant, is a critically endangered plant species with restricted global distribution. Because there is no published report on the in vitro micropropagation of A. chasmanthum, the present study was undertaken to contribute to the development of an efficient micropropagation protocol for its conservation. Seeds collected from the wild showed enhanced germination after being given a chilling treatment (−4 °C and −20 °C) for different durations (10, 20, 30 and 40 days). Seeds given a chilling treatment of −4 °C for 10 days showed enhanced germination rates of 47.59 ± 0.53% with a mean germination time of 10.78 ± 0.21 days compared to seeds kept at room temperature when grown in an MS basal medium. Nodes, leaves and stems, taken from 20–40-day-old seedlings, were used as an explant for micropropagation. An MS medium supplemented with different concentrations of cytokinins (BAP, Kn), auxins (2,4-D, NAA), and an additive adenine sulphate were tested for callusing, direct shoot regeneration and rooting. Only nodal explants responded and showed direct multiple shoot regeneration with 7 ± 0.36 shoots with an elongation of 5.51 ± 0.26 cm in the MS medium supplemented with BAP 0.5 mg/L, and with a response time (RT) of 10.41 ± 0.51 days and a percentage culture response of 77.77 ± 2.77%. Rhizome formation was observed after 8 weeks, with the highest culture response of 36.66 ± 3.33% in the MS basal media with an RT of 43.75 ± 0.50 days. These rhizomes showed a 60% germination rate within 2 weeks and developed into plantlets. The present in vitro regeneration protocol could be used for the large-scale propagation and conservation of A. chasmanthum.
Analyzing the Contribution of Human Mobility to Changes in Air Pollutants: Insights from the COVID-19 Lockdown in Wuhan
Authors: Wu Jiansheng , Qian Yun , Wang Yuan , Wang Na
Published: December 2021

During the COVID-19 lockdown in Wuhan, transportation, industrial production and other human activities declined significantly, as did the NO2 concentration. In order to assess the relative contributions of different factors to reductions in air pollutants, we implemented sensitivity experiments by Random Forest (RF) models, with the comparison of the contributions of meteorological conditions, human mobility, and emissions from industry and households between different periods. In addition, we conducted scenario analyses to suggest an appropriate limit for control of human mobility. Different mechanisms for air pollutants were shown in the pre-pandemic, pre-lockdown, lockdown, and post-pandemic periods. Wind speed and the Within-city Migration index, representing intra-city mobility intensity, were excluded from stepwise multiple linear models in the pre-lockdown and lockdown periods. The results of sensitivity experiments show that, in the COVID-19 lockdown period, 73.3% of the reduction can be attributed to decreased human mobility. In the post-pandemic period, meteorological conditions control about 42.2% of the decrease, and emissions from industry and households control 40.0%, while human mobility only contributes 17.8%. The results of the scenario analysis suggest that the priority of restriction should be given to human mobility within the city than other kinds of human mobility. The reduction in the NO2 concentration tends to be smaller when human mobility within the city decreases by more than 70%. A limit of less than 40% on the control of the human mobility can achieve a better effect, especially in cities with severe traffic pollution.
Pharmacologically Targeting the Fibroblast Growth Factor 14 Interaction Site on the Voltage-Gated Na+ Channel 1.6 Enables Isoform-Selective Modulation
Authors: Dvorak Nolan M. , Tapia Cynthia M. , Singh Aditya K. , Baumgartner Timothy J. , Wang Pingyuan , Chen Haiying , Wadsworth Paul A. , Zhou Jia , Laezza Fernanda
Published: December 2021

Voltage-gated Na+ (Nav) channels are the primary molecular determinant of the action potential. Among the nine isoforms of the Nav channel α subunit that have been described (Nav1.1-Nav1.9), Nav1.1, Nav1.2, and Nav1.6 are the primary isoforms expressed in the central nervous system (CNS). Crucially, these three CNS Nav channel isoforms display differential expression across neuronal cell types and diverge with respect to their subcellular distributions. Considering these differences in terms of their localization, the CNS Nav channel isoforms could represent promising targets for the development of targeted neuromodulators. However, current therapeutics that target Nav channels lack selectivity, which results in deleterious side effects due to modulation of off-target Nav channel isoforms. Among the structural components of the Nav channel α subunit that could be pharmacologically targeted to achieve isoform selectivity, the C-terminal domains (CTD) of Nav channels represent promising candidates on account of displaying appreciable amino acid sequence divergence that enables functionally unique protein–protein interactions (PPIs) with Nav channel auxiliary proteins. In medium spiny neurons (MSNs) of the nucleus accumbens (NAc), a critical brain region of the mesocorticolimbic circuit, the PPI between the CTD of the Nav1.6 channel and its auxiliary protein fibroblast growth factor 14 (FGF14) is central to the generation of electrical outputs, underscoring its potential value as a site for targeted neuromodulation. Focusing on this PPI, we previously developed a peptidomimetic derived from residues of FGF14 that have an interaction site on the CTD of the Nav1.6 channel. In this work, we show that whereas the compound displays dose-dependent effects on the activity of Nav1.6 channels in heterologous cells, the compound does not affect Nav1.1 or Nav1.2 channels at comparable concentrations. In addition, we show that the compound correspondingly modulates the action potential discharge and the transient Na+ of MSNs of the NAc. Overall, these results demonstrate that pharmacologically targeting the FGF14 interaction site on the CTD of the Nav1.6 channel is a strategy to achieve isoform-selective modulation, and, more broadly, that sites on the CTDs of Nav channels interacted with by auxiliary proteins could represent candidates for the development of targeted therapeutics.
A Comparative Study of Koizumi and Longa Methods of Intraluminal Filament Middle Cerebral Artery Occlusion in Rats: Early Corticosterone and Inflammatory Response in the Hippocampus and Frontal Cortex
Authors: Onufriev Mikhail V. , Moiseeva Yulia V. , Zhanina Marina Y. , Lazareva Natalia A. , Gulyaeva Natalia V.
Published: December 2021

Two classical surgical approaches for intraluminal filament middle cerebral artery occlusion (MCAO), the Longa et al. (LM) and Koizumi et al. methods (KM), are used as alternatives in preclinical studies to induce stroke in rodents. Comparisons of these MCAO models in mice showed critical differences between them along with similarities (Smith et al. 2015; Morris et al. 2016). In this study, a direct comparison of MCAO-KM and MCAO-LM in rats was performed. Three days after MCAO, infarct volume, mortality rate, neurological deficit, and weight loss were similar in these models. MCAO-LM rats showed an increase in ACTH levels, while MCAO-KM rats demonstrated elevated corticosterone and interleukin-1β in blood serum. Corticosterone accumulation was detected in the frontal cortex (FC) and the hippocampus of the MCAO-KM group. IL1β beta increased in the ipsilateral hippocampus in the MCAO-KM group and decreased in the contralateral FC of MCAO-LM rats. Differences revealed between MCAO-KM and MCAO-LM suggest that corticosterone and interleukin-1β release as well as hippocampal accumulation is more expressed in MCAO-KM rats, predisposing them to corticosterone-dependent distant neuroinflammatory hippocampal damage. The differences between two models, particularly, malfunction of the hypothalamic–pituitary–adrenal axis, should be considered in the interpretation, comparison, and translation of pre-clinical experimental results.
The Role of the Inflammatory Response in Mediating Functional Recovery Following Composite Tissue Injuries
Authors: Janakiram Naveena B. , Valerio Michael S. , Goldman Stephen M. , Dearth Christopher L.
Published: December 2021

Composite tissue injuries (CTI) are common among US Military Service members during combat operations, and carry a high potential of morbidity. Furthermore, CTI are often complicated due to an altered wound healing response, resulting in part from a dysregulation of the innate and adaptive immune responses. Unlike normal wound healing, in CTI, disruptions occur in innate immune responses, altering neutrophil functions, macrophage activation and polarization, further impacting the functions of T regulatory cells. Additionally, the biological underpinnings of these unfavorable wound healing conditions are multifactorial, including various processes, such as: ischemia, hypoxia, low nutrient levels, and altered cell metabolic pathways, among others, all of which are thought to trigger anergy in immune cells and destabilize adaptive immune responses. As a result, impaired wound healing is common in CTI. Herein, we review the altered innate and adaptive immune cells and their metabolic status and responses following CTI, and discuss the role a multi-pronged immunomodulatory approach may play in facilitating improved outcomes for afflicted patients.
KCa1.1 K+ Channel Inhibition Overcomes Resistance to Antiandrogens and Doxorubicin in a Human Prostate Cancer LNCaP Spheroid Model
Authors: Ohya Susumu , Kajikuri Junko , Endo Kyoko , Kito Hiroaki , Matsui Miki
Published: December 2021

Several types of K+ channels play crucial roles in tumorigenicity, stemness, invasiveness, and drug resistance in cancer. Spheroid formation of human prostate cancer (PC) LNCaP cells with ultra-low attachment surface cultureware induced the up-regulation of cancer stem cell markers, such as NANOG, and decreased the protein degradation of the Ca2+-activated K+ channel KCa1.1 by down-regulating the E3 ubiquitin ligase, FBXW7, compared with LNCaP monolayers. Accordingly, KCa1.1 activator-induced hyperpolarizing responses were larger in isolated cells from LNCaP spheroids. The pharmacological inhibition of KCa1.1 overcame the resistance of LNCaP spheroids to antiandrogens and doxorubicin (DOX). The protein expression of androgen receptors (AR) was significantly decreased by LNCaP spheroid formation and reversed by KCa1.1 inhibition. The pharmacological and genetic inhibition of MDM2, which may be related to AR protein degradation in PC stem cells, revealed that MDM2 was responsible for the acquisition of antiandrogen resistance in LNCaP spheroids, which was overcome by KCa1.1 inhibition. Furthermore, a member of the multidrug resistance-associated protein subfamily of ABC transporters, MRP5 was responsible for the acquisition of DOX resistance in LNCaP spheroids, which was also overcome by KCa1.1 inhibition. Collectively, the present results suggest the potential of KCa1.1 in LNCaP spheroids, which mimic PC stem cells, as a therapeutic target for overcoming antiandrogen- and DOX-resistance in PC cells.
Tobramycin Supplemented Small-Diameter Vascular Grafts for Local Antibiotic Delivery: A Preliminary Formulation Study
Authors: Rosalia Mariella , Ravipati Priusha , Grisoli Pietro , Dorati Rossella , Genta Ida , Chiesa Enrica , Bruni Giovanna , Conti Bice
Published: December 2021

Peripheral artery occlusive disease is an emerging cardiovascular disease characterized by the blockage of blood vessels in the limbs and is associated with dysfunction, gangrene, amputation, and a high mortality risk. Possible treatments involve by-pass surgery using autologous vessel grafts, because of the lack of suitable synthetic small-diameter vascular prosthesis. One to five percent of patients experience vascular graft infection, with a high risk of haemorrhage, spreading of the infection, amputation and even death. In this work, an infection-proof vascular graft prototype was designed and manufactured by electrospinning 12.5% w/v poly-L-lactic-co-glycolic acid solution in 75% v/v dichloromethane, 23.8% v/v dimethylformamide and 1.2% v/v water, loaded with 0.2% w/wPLGA. Polymer and tobramycin concentrations were selected after viscosity and surface tension and after HPLC-UV encapsulation efficiency (EE%) evaluation, respectively. The final drug-loaded prototype had an EE% of 95.58% ± 3.14%, with smooth fibres in the nanometer range and good porosity; graft wall thickness was 291 ± 20.82 μm and its internal diameter was 2.61 ± 0.05 mm. The graft’s antimicrobic activity evaluation through time-kill assays demonstrated a significant and strong antibacterial activity over 5 days against Staphylococcus aureus and Escherichia coli. An indirect cell viability assay on Normal Human Dermal Fibroblasts (NHDF) confirmed the cytocompatibility of the grafts.
S100B Protein as a Therapeutic Target in Multiple Sclerosis: The S100B Inhibitor Arundic Acid Protects from Chronic Experimental Autoimmune Encephalomyelitis
Authors: Camponeschi Chiara , De Carluccio Maria , Amadio Susanna , Clementi Maria Elisabetta , Sampaolese Beatrice , Volonté Cinzia , Tredicine Maria , Romano Spica Vincenzo , Di Liddo Rosa , Ria Francesco , Michetti Fabrizio , Di Sante Gabriele
Published: December 2021

S100B is an astrocytic protein behaving at high concentration as a damage-associated molecular pattern molecule. A direct correlation between the increased amount of S100B and inflammatory processes has been demonstrated, and in particular, the inhibitor of S100B activity pentamidine has been shown to ameliorate clinical scores and neuropathologic-biomolecular parameters in the relapsing-remitting experimental autoimmune encephalomyelitis mouse model of multiple sclerosis. This study investigates the effect of arundic acid (AA), a known inhibitor of astrocytic S100B synthesis, in the chronic experimental autoimmune encephalomyelitis, which is another mouse model of multiple sclerosis usually studied. By the daily evaluation of clinical scores and neuropathologic-molecular analysis performed in the spinal cord, we observed that the AA-treated group showed lower severity compared to the vehicle-treated mice, particularly in the early phase of disease onset. We also observed a significant reduction of astrocytosis, demyelination, immune infiltrates, proinflammatory cytokines expression and enzymatic oxidative reactivity in the AA-treated group. Overall, our results reinforce the involvement of S100B in the development of animal models of multiple sclerosis and propose AA targeting the S100B protein as a focused potential drug to be considered for multiple sclerosis treatment.
Consumer Acceptability and Sensory Profile of Three New Celery (Apium graveolens) Hybrids and Their Parental Genotypes
Authors: Turner Lucy , Wagstaff Carol , Gawthrop Frances , Lignou Stella
Published: December 2021

Celery is a stalky green vegetable that is grown and consumed globally and used in many cuisines for its distinctive taste and flavour. Previous investigations identified the aroma composition of celery and profiled its sensory characteristics using a trained panel; however, evaluation of the sensory characteristics of celery combined with a consumer panel, where consumer preferences and acceptability are determined, is novel. In this study, three parental genotypes (12, 22 and 25) and three new hybrids (12x22, 22x12 and 25x12) were presented to a trained sensory panel (n = 12) for profiling and a consumer panel (n = 118), where liking and preference were assessed. Celery samples were analysed by SPME GC–MS and significant differences in aroma composition between all samples were identified, causing significant differences in the sensory profile. Furthermore, significant differences in attributes assessed for liking (appearance, aroma, texture and overall) were identified. Consumer segmentation identified three groups of consumers exhibiting differences in the hedonic reaction to the samples. Sweet and bitter taste along with overall flavour were identified as drivers of liking. Hybrid 25x12 was found to be the hybrid that exhibited high intensities for most of the attributes assessed.
Primary Cilia Structure Is Prolonged in Enteric Neurons of 5xFAD Alzheimer’s Disease Model Mice
Authors: Nguyen Vu Thu Thuy , Brücker Lena , Volz Ann-Kathrin , Baumgärtner Julia C. , dos Santos Guilherme Malena , Valeri Francesco , May-Simera Helen , Endres Kristina
Published: December 2021

Neurodegenerative diseases such as Alzheimer’s disease (AD) have long been acknowledged as mere disorders of the central nervous system (CNS). However, in recent years the gut with its autonomous nervous system and the multitude of microbial commensals has come into focus. Changes in gut properties have been described in patients and animal disease models such as altered enzyme secretion or architecture of the enteric nervous system. The underlying cellular mechanisms have so far only been poorly investigated. An important organelle for integrating potentially toxic signals such as the AD characteristic A-beta peptide is the primary cilium. This microtubule-based signaling organelle regulates numerous cellular processes. Even though the role of primary cilia in a variety of developmental and disease processes has recently been recognized, the contribution of defective ciliary signaling to neurodegenerative diseases such as AD, however, has not been investigated in detail so far. The AD mouse model 5xFAD was used to analyze possible changes in gut functionality by organ bath measurement of peristalsis movement. Subsequently, we cultured primary enteric neurons from mutant mice and wild type littermate controls and assessed for cellular pathomechanisms. Neurite mass was quantified within transwell culturing experiments. Using a combination of different markers for the primary cilium, cilia number and length were determined using fluorescence microscopy. 5xFAD mice showed altered gut anatomy, motility, and neurite mass of enteric neurons. Moreover, primary cilia could be demonstrated on the surface of enteric neurons and exhibited an elongated phenotype in 5xFAD mice. In parallel, we observed reduced β-Catenin expression, a key signaling molecule that regulates Wnt signaling, which is regulated in part via ciliary associated mechanisms. Both results could be recapitulated via in vitro treatments of enteric neurons from wild type mice with A-beta. So far, only a few reports on the probable role of primary cilia in AD can be found. Here, we reveal for the first time an architectural altered phenotype of primary cilia in the enteric nervous system of AD model mice, elicited potentially by neurotoxic A-beta. Potential changes on the sub-organelle level—also in CNS-derived neurons—require further investigations.
Characterization of a Novel Thermostable Dye-Linked l-Lactate Dehydrogenase Complex and Its Application in Electrochemical Detection
Authors: Satomura Takenori , Uno Kohei , Kurosawa Norio , Sakuraba Haruhiko , Ohshima Toshihisa , Suye Shin-ichiro
Published: December 2021

Flavoenzyme dye-linked l-lactate dehydrogenase (Dye-LDH) is primarily involved in energy generation through electron transfer and exhibits potential utility in electrochemical devices. In this study, a gene encoding a Dye-LDH homolog was identified in a hyperthermophilic archaeon, Sulfurisphaera tokodaii. This gene was part of an operon that consisted of four genes that were tandemly arranged in the Sf. tokodaii genome in the following order: stk_16540, stk_16550 (dye-ldh homolog), stk_16560, and stk_16570. This gene cluster was expressed in an archaeal host, Sulfolobus acidocaldarius, and the produced enzyme was purified to homogeneity and characterized. The purified recombinant enzyme exhibited Dye-LDH activity and consisted of two different subunits (products of stk_16540 (α) and stk_16550 (β)), forming a heterohexameric structure (α3β3) with a molecular mass of approximately 253 kDa. Dye-LDH also exhibited excellent stability, retaining full activity upon incubation at 70 °C for 10 min and up to 80% activity after 30 min at 50 °C and pH 6.5–8.0. A quasi-direct electron transfer (DET)-type Dye-LDH was successfully developed by modification of the recombinant enzyme with an artificial redox mediator, phenazine ethosulfate, through amine groups on the enzyme’s surface. This study is the first report describing the development of a quasi-DET-type enzyme by using thermostable Dye-LDH.