bluuebunny/crossref_metadata_2025 huggingface dataset:
Farmers’ Socioeconomic Characteristics and Perception of Land Use Change Defining Optimal Agroforestry Practices in Khost Province, Afghanistan
Authors: Ahmadzai Mujib Rahman , Ismail Mohd Hasmadi , Zaki Pakhriazad Hassan , Magiman Mohd. Maulana , Bawon Paiman
Published: October 2024

Agroforestry practices evolve with the development of basic and advanced facilities, changes in natural and artificial factors of land, and land use trade-offs. This study aims to examine the farmers’ socioeconomic characteristics and perception of land use changes that define optimal agroforestry practices in Khost Province, Afghanistan. Data were collected from 662 farmers and analyzed using univariate Analysis of Variance (ANOVA) and Multivariate Analysis of Variance (MANOVA). The results found that forest and vegetable products, including fruits, berries, herbs, mushrooms, wild animals, oils, wood, honey, okra, eggplant, carrot, cucumber, pine nuts, pepper, and timber, have different impacts in terms of satisfaction with basic and advanced facilities, knowledge of land use changes, satisfaction with natural and artificial resources of land, and barriers to and economic benefits of land use. The limitations of this study included an absence of exogenous factors in the model such as climate change, financial conditions, market fluctuations, regulatory system, the area in which this study is selected, research design, and current condition of endogenous factors. Overall, this study defined a set of optimal agroforestry practices (expressed as crops and products) based on the farmers’ perception of land use changes in Khost Province, Afghanistan. This study provided useful insights for policymakers and development practitioners to promote agroforestry practice adoption and improve the socioeconomic development of agroforestry-dependent communities. Future works could explore the implications of agroforestry practices on the socioeconomic development of other dependent communities in Afghanistan.
Analyzing the Impact of EU’s Legality Requirements Policies on Sustainable Timber and Sawnwood Trade—Focusing on Tropical Wood Trade
Authors: Kim Dong Hyun , Ahn Byeong Il , Shim Gyuhun
Published: October 2024

This study analyzes the impact of the EU′s policy to prohibit the import of illegally harvested timber on the trade of tropical timber and sawnwood. The analysis uses a difference-in-differences approach based on gravity models, with panel data from over 193 countries that trade tropical timber and sawnwood. The result of the analysis shows that the European Union Timber Regulation (EUTR) reduces the trade of illegally harvested timber. It further suggests that the EUTR caused a relatively larger reduction in tropical sawnwood (−0.21%) than that of tropical timber (0.07%). In addition, the Voluntary Partnership Agreement (VPA) appeared to cause a significant reduction in tropical sawnwood (−0.07~−0.05%), whereas tropical timber did not appear to have been significantly impacted. In particular, the reduction in timber exports in countries that have signed the VPA, which aims to encourage exports of legally produced timber, has significant implications for both the EU and timber exporters preparing for the VPA, as both parties strive to expand the VPA. The results of this study suggest that the EU needs to make additional efforts to address the decline in exports from countries that signed the VPA.
The Impact of Different Types of Trees on Annual Thermal Comfort in Hot Summer and Cold Winter Areas
Authors: Chen Xiao , Li Zilong , Wang Zhenyu , Li Jiayu , Zhou Yihua
Published: October 2024

Trees positively improve the annual thermal comfort of the built environment in tropical areas, where climate change is slight throughout the year. However, for areas with high changes in climate all year, the current studies have only explored the summer cooling performance of trees without the impact of different types of trees on annual thermal comfort, especially in cold seasons. Therefore, to quantify the impacts and scientifically guide the optimization of green space layout in hot summer and cold winter areas, this study selected Changsha City as the study area and analyzed how the annual thermal comfort is affected by evergreen trees and deciduous trees, which are two common types of trees in hot summer and cold winter areas. The analytical results indicated that the difference in the effect of deciduous and evergreen trees on outdoor thermal comfort was insignificant in summer, where the difference in the monthly mean PET for the three summer months was slight, being 0.28 °C, 0.14 °C, and 0.29 °C, respectively. However, evergreen trees greatly exacerbated winter cold compared to deciduous trees, with a monthly mean PET decrease by nearly 1.0 °C and an hourly PET reduced by up to 3.57 °C. The difference is mainly attributed to the absorption and reflection of solar radiation by the tree canopy, as well as the cooling and humidifying effect of the tree leaf. In hot summer and cold winter areas, outdoor thermal comfort is still in the “comfortable” and “slightly warm” acceptable stage despite the warming effect of deciduous trees in the spring and autumn seasons. Planting evergreen trees is an inevitable thermal mitigation choice for tropical areas. However, for the areas with high annual climate change, such as hot summer and cold winter areas in China, a change in empirical tree planting patterns and selecting deciduous trees where appropriate will improve year-round outdoor thermal comfort.
Mapping Forest Parameters to Model the Mobility of Terrain Vehicles
Authors: Mikita Tomáš , Rybansky Marian , Krausková Dominika , Dohnal Filip , Vystavěl Ondřej , Hollmannová Sabina
Published: October 2024

This study aims to evaluate the feasibility of using non-contact data collection methods—specifically, UAV (unmanned aerial vehicle)-based and terrestrial laser scanning technologies—to assess forest stand passability, which is crucial for military operations. The research was conducted in a mixed forest stand in the Březina military training area, where the position of trees and their DBHs (Diameter Breast Heights) were recorded. The study compared the effectiveness of different methods, including UAV RGB imaging, UAV-LiDAR, and handheld mobile laser scanning (HMLS), in detecting tree positions and estimating DBH. The results indicate that HMLS data provided the highest number of detected trees and the most accurate positioning relative to the reference measurements. UAV-LiDAR showed better tree detection compared to UAV RGB imaging, though both aerial methods struggled with canopy penetration in densely structured forests. The study also found significant variability in DBH estimation, especially in complex forest stands, highlighting the challenges of accurate tree detection in diverse environments. The findings suggest that while current non-contact methods show promise, further refinement and integration of data sources are necessary to improve their applicability for assessing forest passability in military or rescue contexts.
Oat Okara Fermentation: New Insights into the Microbiological and Metabolomic Characterization
Authors: Meanti Federica , Mussio Chiara , Rocchetti Gabriele , Rebecchi Annalisa , Lucini Luigi , Morelli Lorenzo
Published: October 2024

The importance of the valorization of industrial by-products has led to increasing research into their reuse. In this research, the innovative by-product okara oat flour, derived from the vegetable beverage industry, was studied. Oat okara sourdough was also produced and evaluated. The microbiological identification and typing involved bacterial and yeast isolates from both flour and sourdough. Untargeted metabolomics allowed the identification of biomarkers of fermented flour, such as phenolic classes, post-fermentation metabolites, fatty acids, and amino acids. The microorganisms most found were Weissella confusa, Enterococcus faecium, Pediococcus pentosaceus, and Pichia kudriavzevii, while Saccharomyces cerevisiae appeared only at the end of the sourdough’s back-slopping. Untargeted metabolomics identified a total of 539 metabolites, including phenolic compounds, lipids, amino acids, and organic acids. An increase in polyphenols released from the food matrix was detected, likely because of the higher bio-accessibility of phenolic metabolites promoted by microbial fermentation. Fermentation led to an increase in isoferulic acid, p-coumaric acid, sinapic acid, and a decrease in amino acids, which can be attributed to the metabolism of lactic acid bacteria. Some key markers of the fermentation process of both lactic acid bacteria and yeast were also measured, including organic acids (lactate, succinate, and propionate derivatives) and flavor compounds (e.g., diacetyl). Two bioactive compounds, such as gamma-aminobutyric acid and 3-phenyl-lactic acid had accumulated at the end of fermentation. Taken together, our findings showed that oat okara flour can be considered an excellent raw material for formulating more sustainable and functional foods due to fermentation promoted by autochthonous microbiota.
Using Precious Metals to Reduce the Downside Risk of FinTech Stocks
Authors: Sadorsky Perry
Published: October 2024

FinTech stocks are an important new asset class that reflects the rapidly growing FinTech sector. This paper studies the practical implications of using gold, silver, and basket-of-precious-metals (gold, silver, platinum, palladium) ETFs to diversify risk in FinTech stocks. Downside risk reduction is estimated using relative risk ratios based on CVaR. The analysis shows that gold provides the most downside risk protection. For a 5% CVaR, a 30% portfolio weight for gold reduces the downside risk by about 25%. The minimum variance and minimum correlation three-asset (FinTech, gold, and silver) portfolios (with portfolio weights estimated using a TVP-VAR model) have the highest risk-adjusted returns (Sharpe ratio, Omega ratio) followed by the fixed-weight FinTech and gold portfolio. These results show the benefits of diversifying an investment in FinTech stocks with precious metals. These results are robust to weekly or monthly portfolio rebalancing and reasonable transaction costs.
Socio-Economic Determinants of Human Negligence in Wildfire Incidence: A Case Study from Pakistan’s Peri-Urban and Rural Areas
Authors: Daraz Umar , Bojnec Štefan , Khan Younas
Published: October 2024

This study aims to examine the socio-economic determinants of human negligence in wildfire occurrences across Pakistan’s peri-urban and rural regions. Increasingly frequent and severe wildfires, driven by climate change, socio-economic conditions, and human negligence, have become a pressing issue. Rising global temperatures and changing precipitation patterns have created drier conditions, while unsafe human activities—such as improper disposal of flammable materials and unsafe agricultural burning—further escalate wildfire risks. These issues are particularly pronounced in Pakistan, where high poverty levels, limited resources, and low education contribute to dangerous behaviors. Weak governance and poor policy enforcement further exacerbate the problem. Data were collected using a cross-sectional survey from 500 participants across five regions. Multiple regression analysis revealed that higher poverty levels significantly increased negligence, whereas higher education and improved access to resources reduced it. Structural Equation Modeling (SEM) highlighted the critical roles of local governance, policy enforcement, and community engagement in mitigating wildfires. Correlation analysis indicated an inverse relationship between wildfire risk awareness and negligent behavior. Chi-square tests demonstrated a strong connection between wildfires and migration patterns, underscoring the socio-economic instability caused by these events. Finally, linear regression showed that wildfires significantly impact regional climate indicators, emphasizing the need for integrated management strategies. This study offers valuable insights into the socio-economic factors driving wildfires in Pakistan and provides guidance for developing targeted mitigation strategies.
Volatile Organic Compounds as Early Detection Indicators of Wheat Infected by Sitophilus oryzae
Authors: Liu Xinjie , Jiang Haixin , Xu Haoqi , Shang Sijia , Wang Dianxuan , Bai Yueliang , Zeng Fangfang
Published: October 2024

The rice weevil, Sitophilus oryzae (L.), is a major pest that poses a considerable threat to grain safety storage. Early detection is of great significance in reducing grain losses. Studies have demonstrated that pest infestation causes alterations in grain volatiles, potentially indicating the presence of pests. In this study, we detected volatile organic compounds (VOCs) in non-infected and pest-infected wheat on the 3rd, 9th, 17th, 22nd, and 40th days, corresponding to the developmental stages of the rice weevil at the egg, young larval, old larval, pupal, and adult stages, respectively. A total of 126 VOCs were identified, including 96 hydrocarbons, 7 alcohols, 5 aldehydes, 9 ketones, 9 esters, and 18 other compounds, 62 of which are newly produced compared to non-infected wheat. Six characteristic volatiles, namely dodecane, pentadecane, hexadecane, heptadecane, 2, 6, 10-trimethylpentadecane, and squalene, may be related to the degradation of lipids and the expression of wheat stress tolerance and underwent significant changes as infestation progressed, according to the VIP value. This study assists in interpreting the effects of rice weevil infestation on wheat at the metabolic level and establishes a foundation for storage inspection based on VOC analysis.
Goat Milk Protein-Derived ACE Inhibitory Peptide SLPQ Exerts Hypertension Alleviation Effects Partially by Regulating the Inflammatory Stress of Endothelial Cells
Authors: Xing Shenghao , Zhang Xiaotong , Mu Tong , Cao Jianxin , Zhao Ke , Han Bing , Peng Xinyan
Published: October 2024

Hypertension has always posed a severe threat to people’s health. Food-derived angiotensin-converting enzyme (ACE)-inhibitory peptides have the potential to both prevent and treat hypertension. In the current investigation, two ACE-inhibitory peptides (SLPQ and PYVRYL) from goat milk were studied for their endothelial effects using EA.hy926 cells. PYVRYL outperformed SLPQ, yet neither impacted cell survival below 200 μg/mL. Investigation of SLPQ’s impact on EA.hy926 cell expression revealed 114 differentially expressed genes, with 65 downregulated and 49 upregulated. The genes were enriched in cytokine interactions, coagulation cascades, Hippo signaling, and ECM–receptor interaction. Decreased c-x-c motif chemokine ligand 2 (CXCL2), integrin subunit beta 2 (ITGB2), and fbj murine osteosarcoma viral oncogene homologue (FOS) expression and increased secreted phosphoprotein 1 (SPP1) expression may protect endothelial cells from inflammation. Our findings suggest that beyond ACE inhibition, SLPQ aids blood pressure control by influencing endothelial function, paving the way for its use as an antihypertensive food ingredient.
Detection of AFB1 by Immunochromatographic Test Strips Based on Double-Probe Signal Amplification with Nanobody and Biotin–Streptavidin System
Authors: Li Yifan , Li Zhenfeng , Jia Baozhu , Tu Zhui , Zeng Juntao , Pang Jiarui , Ren Wenjie , Huang Zhibing , He Baoshan , Wang Zhihua
Published: October 2024

Aflatoxin B1 (AFB1) is highly toxic and difficult to prevent. It is mainly produced by fungi and exists in plants and animals and is classified by the World Health Organization as a class I carcinogen, posing a serious threat to human and animal health. Therefore, it is important to establish an efficient, sensitive, and on-site detection method for AFB1 to protect human health. The immunochromatographic test strip method is simple, sensitive, and can achieve real-time detection. However, traditional immunochromatographic test strips have low sensitivity due to their relatively weak optical properties. In this study, Nb-G8 was biotinylated using a chemical method. Two sizes of gold nanoflowers (AuNFs) were prepared and combined with biotinylated G8 and streptavidin to form two types of probes. These probes were sprayed on gold standard pads and expanded pads, respectively, to enhance the signals through the high affinity interaction between streptavidin and biotin. Under the optimal experimental conditions, the half maximal inhibitory concentration (IC50) of this method was 5.0 ng/mL and the limit of detection (IC10) was 0.03 ng/mL, which increased the sensitivity of the test strip by four-fold compared with that of the traditional biotinylated nanoantibody immunochromatography test strip and had a wider detection range. In conclusion, the use of a high-affinity amplification signal between biotin and streptavidin is a valuable method for the detection of aflatoxin.
Widely Targeted Metabolomics Analysis Revealed the Component Differences of Hemerocallis citrina Borani in Different Production Areas of Datong
Authors: Fu Yongxia , Wang Haizhen , Liu Zhenyu , Wang Han , Zhao Mengying , Li Zhihao , Guo Shang
Published: October 2024

Hemerocallis citrina Borani (H. citrina) has garnered significant attention due to its abundant nutritional quality. Datong, located in Shanxi Province, is recognized as one of the four major production regions for high-quality H. citrina. While Datong boasts multiple production areas, the nutritional composition of daylilies varies across regions due to environmental factors and planting patterns, which remain unclear. This study focuses on the total polyphenol and flavonoid contents (TPCs and TFCs) and protein content of H. citrina from three areas in Datong: Sanshilipu (DTSSLP), Dangliuzhuang (DTDLZ), and Jijiazhuang (DTJJZ). Additionally, a widely targeted metabolomics analysis was used to analyze the metabolite composition of H. citrina from these three areas. The results showed that H. citrina in DTSSLP had the highest contents of protein and amino acids, as well as TPCs and TFCs. A total of 798 differential metabolites were identified in H. citrina across the areas, with DTSSLP showing the highest levels of different classifications of metabolites, indicating its enhanced health benefits and physiological activities. Nine metabolic pathways were related with the different characteristics among DTSSLP, DTDLZ, and DTJJZ. This study provides theoretical support for distinguish H. citrina from different producing regions and elucidates the mechanisms underlying its metabolic pathways.
Multi-Buoy Deployment Method Based on an Improved Tuna Swarm Optimizer Enhanced with Fractional-Order Calculus Method for Marine Observation
Authors: Ren Ranzhen , Zhang Lichuan , Pan Guang , Zhang Xiaomeng , Liu Lu , Han Guangyao
Published: October 2024

Ocean buoys play a critical role in marine hydrological, water quality, and meteorological monitoring, with applications in navigation, environmental observation, and communication. However, accurately modeling and deploying a multi-buoy system in the complex marine environment presents significant challenges. To address these challenges, this study proposes an enhanced deployment strategy using the tuna swarm optimizer enhanced with the fractional-order calculus method for marine observation. The proposed method first introduces a detailed observation model that precisely captures the performance of buoys in terms of coverage and communication efficiency. By integrating the observation coverage ratio and communication energy consumption, we establish an optimal multi-buoy deployment model. The proposed method leverages tent chaotic mapping to improve the diversity of initial solution generation and incorporates fractional-order calculus to strengthen its search capabilities. Simulation experiments and statistical analysis verify the effectiveness of the proposed deployment model, with the proposed method achieving the best performance in deploying the multi-buoy system, reaching a final fitness value of 0.190052 at iteration 449, outperforming TSA, PSO, GWO, and WOA. These results highlight the potential of the proposed method in optimizing multi-buoy system deployment in marine observation.
An Error Analysis Study for the Distributed-Order Time-Fraction Model Using an Efficient Numerical Method
Authors: Kosari Saeed , Guan Hao , Derakhshan MohammadHossein
Published: October 2024

In this paper, we propose a numerical scheme based on the shifted Legendre polynomials for solving the forced Korteweg–de Vries (fKdV) equation including a Caputo fractional operator of a distributed order. To obtain numerical solutions of these types of equations, we derive an operational matrix based on the shifted Legendre polynomials, and using this operational matrix, their equations change to a set of nonlinear algebraic systems. Then, by calculating these systems in the collocation points, we solve systems. Also, convergence and error are investigated in this paper. Finally, several numerical examples to show the applicability of our scheme are displayed.
Finite-Time Projective Synchronization in Fractional-Order Inertial Memristive Neural Networks: A Novel Approach to Image Encryption
Authors: Weng Huixian , Yang Yongqing , Hao Rixu , Liu Fengyi
Published: October 2024

This paper presents a novel finite-time projective synchronization (FTPS) control strategy for fractional-order inertial memristive neural networks (FOIMNNs), exploring its application in image encryption. A sufficient condition for ensuring FTPS in FOIMNNs is established and validated through numerical simulations. These simulations indicate that the proposed strategy provides reliable synchronization performance. Furthermore, an efficient method for image encryption was developed, potentially improving data security. Comparative analyses with existing methods suggest that this approach could offer incremental benefits in secure communication and data protection.
Editorial: Genetics and Genomics of Polyploid Plants
Authors: D’Agostino Nunzio , Fasano Carlo
Published: October 2024

Polyploidy, the condition of having more than two complete sets of chromosomes, is a widespread and influential phenomenon in the plant kingdom [...]
Dancing Towards the End—Ecological Oscillations in Mediterranean Coral Reefs Prior to the Messinian Salinity Crisis (Calcare di Rosignano Formation, Acquabona, Tuscany, Italy)
Authors: Coletti Giovanni , Vimercati Alberto , Bosellini Francesca R. , Collareta Alberto , Bosio Giulia , Guido Adriano , Vescogni Alessandro , Basso Daniela , Bialik Or M.
Published: October 2024

The lower Messinian Calcare di Rosignano Formation (Tuscany, Italy, 43° N) preserves one of the youngest and northernmost examples of coral reefs in the Mediterranean. The outcropping succession of the Acquabona quarry consists of four main facies, namely, in ascending stratigraphic order: (1) coral boundstone, (2) coralline algal rudstone, (3) serpulid floatstone to packstone, and (4) peloidal packstone to grainstone. The succession displays a trend toward increasingly more shallow conditions and progressively more restricted water circulation. The coral reef displays a limited coral biodiversity and a remarkable abundance of heterotrophs, similar to modern coral reefs developed at the edges of the ecological niche of symbiont-bearing colonial corals. The widespread presence of coral colonies pervasively encrusted by coralline algae and benthic foraminifera suggests that short-term environmental perturbations caused temporary shutdowns of the coral-dominated carbonate factory. Moving upwards, there are fewer corals and more highly adaptable carbonate producers like coralline algae and serpulids. This suggests that the decline of corals had been caused by the conditions in the basin becoming more stressful, up to the collapse of the coral community. The overall succession indicates that coral-dominated ecosystems located at the edges of the coral zone are very sensitive; they can be affected even by minor perturbations and easily collapse if negative conditions persist.
Evaluating Muscle Mass Changes in Critically Ill Patients: Rehabilitation Outcomes Measured by Ultrasound and Bioelectrical Impedance
Authors: Kim Mijoo , Kim Soyun , Ju Yerin , Ahn Soyoung , Lee Song I
Published: October 2024

Background/Objectives: The loss of muscle mass is common in critically ill patients and is associated with poor prognosis, and efforts have been made to mitigate muscle loss through rehabilitation. This study aimed to evaluate changes in muscle mass in critically ill patients following rehabilitation. Methods: We enrolled 53 patients expected to stay in the ICU for more than 7 days, dividing them into rehabilitation (15 patients) and no rehabilitation groups (38 patients). Muscle mass was measured using ultrasound and bioelectrical impedance analysis (BIA). Results: Baseline characteristics and comorbidities showed no statistical differences between the two groups. Initial measurements of muscles showed no significant differences between the groups in rectus femoris thickness, total anterior thigh muscle thickness, cross-sectional area, echogenicity, or in-body skeletal muscle mass at baseline and 7 days. However, at 14 days, significant differences emerged. The rehabilitation group had greater rectus femoris thickness (1.42 cm vs. 0.81 cm, p = 0.007) and total anterior thigh muscle thickness (3.79 cm vs. 2.32 cm, p = 0.007) compared to the no rehabilitation group. Additionally, the rehabilitation group experienced a significantly smaller reduction in rectus femoris cross-sectional area (−4.6% vs. −22.8%, p = 0.021). Although survival rates were higher in the rehabilitation group (73.3% vs. 52.6%), this difference was not statistically significant (p = 0.096). Conclusions: Our findings suggest that rehabilitation in critically ill patients is associated with a slower rate of muscle loss, particularly in the cross-sectional area of the rectus femoris muscle, which may be beneficial for patient recovery.
Prevalence and Factors of Pregnancy Termination Among Reproductive-Aged Women: Evidence from the Bangladesh Demographic and Health Survey
Authors: Islam Md. Rabiul , Rahman Makfiratur , Tanha Arifa Farzana , Sheba Nusrat Hossain , Haque S. M. Raysul , Baset Md. Kamran ul , Hossain Zenat Zebin , Gani Mohammad Abbas , Hannan J. M. A.
Published: October 2024

Background: Pregnancy termination (PT) is a major public health concern in low-and middle-income countries like Bangladesh. This cross-sectional study aimed to determine the prevalence and factors of PT using the nationally representative Bangladesh Demographic and Health Survey data 2017–2018. Materials and Methods: A weighted population-based sample of 8759 ever-married reproductive-aged women (15–49 years) was included in the study. The outcome variable was PT in any of the following forms: miscarriage, induced abortion, and stillbirth. A univariate analysis for mean, frequency, and percentage and multiple logistical regression were used to determine the factors associated with PT. Results: Around 18% of the women were found to have PT. The mean age of the women in the study was 25.79 years; 65.1% lived in the rural areas, and the majority of them were Muslims. Advanced age of the women (AOR:3.49, p = 0.004), residence in the countryside (AOR:0.81, p = 0.002), higher education (AOR:0.72, p = 0.027), not being a Muslim (AOR:0.74, p = 0.010), higher socio-economic status (AOR:1.28, p = 0.027), having a job (AOR:1.15, p = 0.041), being married at the age of >22 years (AOR:0.71, p = 0.036), and using a mobile phone (AOR:1.22, p = 0.002) were significant factors of PT. This study did not find any association between PT and contraceptive use. Conclusions: Age, living region, education, religion, wealth index, working status, marital age, and mobile phone use are the determinants of PT. Interventions including these factors need to be made to reduce PT in Bangladeshi women. These findings could be helpful in undertaking further epidemiological studies to understand the actual causes of PT in various rural and urban settings among different socio-demographic groups in Bangladesh.
Toward Utilizing Similarity in Hydrologic Data Assimilation
Authors: Lee Haksu , Shen Haojing , Liu Yuqiong
Published: October 2024

Similarity to reality is a necessary property of models in earth sciences. Similarity information can thus possess a large potential in advancing geophysical modeling and data assimilation. We present a formalism for utilizing similarity within the existing theoretical data assimilation framework. Two examples illustrate the usefulness of utilizing similarity in data assimilation. The first, theoretical example shows changes in the accuracy of the amplitude estimate in the presence of a phase error in a sine function, where correcting the phase error prior to the assimilation reduces the degree of ill-posedness of the assimilation problem. This signifies the importance of accounting for the phase error in order to reduce the error in the amplitude estimate of the sine function. The second, real-world example illustrates that timing errors in simulated flow degrade the data assimilation performance, and that the flow gradient-informed shifting of rainfall time series improved the assimilation results with less adjusting model states. This demonstrates the benefit of utilizing streamflow gradients in shifting rainfall time series in a way to improve streamflow timing—vital information for flood early warning and preparedness planning. Finally, we discuss the implications, potential issues, and future challenges associated with utilizing similarity in hydrologic data assimilation.
A Systematic Review of the Current State of Numerical Groundwater Modeling in American Countries: Challenges and Future Research
Authors: Lozano Hernández Baltazar Leo , Marín Celestino Ana Elizabeth , Martínez Cruz Diego Armando , Ramos Leal José Alfredo , Hernández Pérez Eliseo , García Pazos Joel , Almanza Tovar Oscar Guadalupe
Published: October 2024

In arid and semi-arid regions, groundwater is often the only available water source. However, overexploitation and pollution have led to a decrease in groundwater quantity and quality. Therefore, the proper management of groundwater resources is essential to promote sustainable development. Numerical simulation models (NSMs) have emerged as a valuable tool to address these challenges due to their ability to accurately and efficiently model groundwater systems. This study provides a comprehensive systematic review to evaluate the current knowledge on using numerical groundwater flow models for planning and water resource management in countries in the American region. A total of 166 research articles were published between the years 2000 and 2024. We analyzed and summarized details such as the study regions, numerical simulation methods and applied software, performance metrics, modeling units, modeling limitations, and prediction scenarios. In addition, we discuss alternatives to address the constraints and difficulties and suggest recommendations for future research. The continued research, improvement, and development of numerical groundwater models are essential to ensure the sustainability of groundwater resources.
Relationships Among COVID-19-Related Service Uptake, HIV Status, Drug Use, and COVID-19 Antibody Status Among HIV Testing Intervention Participants in KwaZulu-Natal, South Africa
Authors: Williams Leslie D. , Memela Phumlani , van Heerden Alastair , Friedman Samuel R. , Joseph Phillip , Chibi Buyisile
Published: October 2024

People living with HIV (PLWH) and people who use drugs are vulnerable populations who may face barriers to accessing health services and may have irregularities in immune function. People with undiagnosed HIV infection may be particularly likely to have compromised immune function. However, research about whether/how HIV status is related to COVID-19-related health outcomes has been equivocal, and research on the predictors of COVID-19-related health service access/uptake has been limited in Sub-Saharan African settings. Among 470 participants of a peer-recruitment-based HIV-testing intervention in KwaZulu-Natal, we examined whether HIV status and/or hard drug use were associated with uptake of COVID-19 testing and vaccination, and whether they moderated the relationship between COVID-19 vaccination status and COVID-19 IgG antibody status. Women were significantly more likely than men to report testing for COVID-19 (OR = 1.84; p = 0.002) and being vaccinated (OR = 1.79; p = 0.002). Neither HIV status nor drug use was associated with likelihood of getting tested or vaccinated. Vaccinated participants (90% of whom obtained vaccines more than 6 months before the study) were significantly more likely to test positive for COVID-19 IgG antibodies (OR = 6.86; p < 0.0005). This relationship held true for subgroups of PLWH and participants with previously undiagnosed/uncontrolled HIV infection, and was not moderated by HIV status or hard drug use. These findings may suggest that both people who use drugs and PLWH were served as well as other people by KwaZulu-Natal’s COVID-19 response. However, gender-based disparities in COVID-19 service uptake suggest that special care should be taken during future COVID-19 outbreaks or other new epidemics to improve access to related healthcare services among men in this region.
The Absence of Thyroid-Stimulating Hormone Receptor Expression on Natural Killer T Cells: Implications for the Immune–Endocrine Interaction
Authors: Adamska-Fita Emilia , Śliwka Przemysław Wiktor , Karbownik-Lewińska Małgorzata , Lewiński Andrzej , Stasiak Magdalena
Published: October 2024

The expression of thyroid-stimulating hormone receptor (TSHR) has been documented on various immune cells, including B lymphocytes, T lymphocytes, Natural Killer (NK) cells, monocytes, and dendritic cells (DCs). Natural Killer T (NKT) cells serve as a crucial link between innate and adaptive immunity, playing significant roles in immunological interactions and autoimmune diseases. The aim of the present study was to evaluate the presence of TSHR on NKT cells. Our research involved patients with thyroid disease, as well as healthy controls. Peripheral blood mononuclear cells (PBMCs) and, thereafter, NKT cells were isolated from 86 patients with benign nodular thyroid disease with and without autoimmune thyroid disease (AITD) (28 and 56 cases, respectively), and TSHR expression was analyzed using fluorescence-activated cell sorting (FACS). In order to confirm the results, the reverse-transcription polymerase chain reaction (RT-PCR) method was used in cells obtained from healthy individuals. Our findings obtained with application of the FACS method revealed that TSHR is not expressed on NKT cells in either AITD or non-AITD patients, though TSHR was detected in the total PBMC population (TSHR+ cells 2.77%). The absence of TSHR on NKT cells was further confirmed with RT-PCR in healthy individuals (p < 0.0001). These results questioned the previously suggested direct influence of NKT cells on AITD development.
Knockout of the Carbohydrate Responsive Element Binding Protein Enhances Proliferation and Tumorigenesis in Renal Tubules of Mice
Authors: Hansen Kerrin , Peters Kristin , Burkert Christian K. , Brose Eric , Calvisi Diego F. , Ehricke Katrina , Engeler Maren , Knuth Elisa , Kröger Nils , Lohr Andrea , Prey Jessica , Sonke Jenny , Vakeel Padmanabhan , Wladasch Juliane , Zimmer Jenny , Dombrowski Frank , Ribback Silvia
Published: October 2024

Glycogen-storing so-called clear cell kidney tubules (CCTs), precursor lesions of renal cell carcinoma, have been described in diabetic rats and in humans. The lesions show upregulation of the Akt/mTOR-pathway and the related transcription factor carbohydrate responsive element binding protein (ChREBP), which is supposedly pro-oncogenic. We investigated the effect of ChREBP-knockout on nephrocarcinogenesis in streptozotocin-induced diabetic and normoglycemic mice. Diabetic, but not non-diabetic mice, showed CCTs at 3, 6 and 12 months of age. Glycogenosis was confirmed by periodic acid schiff reaction and transmission electron microscopy. CCTs in ChREBP-knockout mice consisted of larger cells and occurred more frequently compared to wildtype mice. Progression towards kidney tumors was observed in both diabetic groups but occurred earlier in ChREBP-knockout mice. Proliferative activity assessed by BrdU-labeling was lower in 1-week-old but higher in 12-month-old diabetic ChREBP-knockout mice. Surprisingly, renal neoplasms occurred spontaneously in non-diabetic ChREBP-knockout, but not non-diabetic wildtype mice, indicating an unexpected tumor-suppressive function of ChREBP. Immunohistochemistry showed upregulated glycolysis and lipogenesis, along with activated Akt/mTOR-signaling in tumors of ChREBP-knockout groups. Immunohistochemistry of human clear cell renal cell carcinomas revealed reduced ChREBP expression compared to normal kidney tissue. However, the molecular mechanisms by which loss of ChREBP might facilitate tumorigenesis require further investigation.
Investigating the Impact of the Parkinson’s-Associated GBA1 E326K Mutation on β-Glucocerebrosidase Dimerization and Interactome Dynamics Through an In Silico Approach
Authors: Pietrafesa Davide , Casamassa Alessia , Benassi Barbara , Santoro Massimo , Marano Massimo , Consales Claudia , Rosati Jessica , Arcangeli Caterina
Published: October 2024

Heterozygous mutations or genetic variants in the GBA1 gene, which encodes for the β-glucocerebrosidase (GCase), a lysosomal hydrolase enzyme, may increase the risk of Parkinson’s disease (PD) onset. The heterozygous E326K form is one of the most common genetic risk factors for PD worldwide, but, to date, the underlying molecular mechanisms remain unclear. Here, we investigate the effect of the E326K on the structure, stability, dimerization process, and interaction mode with some proteins of the interactome of GCase using multiple molecular dynamics (MD) simulations at pH 5.5 and pH 7.0 to mimic the lysosomal and endoplasmic reticulum environments, respectively. The analysis of the MD trajectories highlights that the E326K mutation did not significantly alter the structural conformation of the catalytic dyad but significantly makes the structure of the dimeric complexes unstable, especially at lysosomal pH, potentially impacting the organization of the quaternary structure. Furthermore, the E326K mutation significantly impacts protein interactions by altering the binding mode with the activator Saposin C (SapC), reducing the binding affinity with the inhibitor α-Synuclein (α-Syn), and increasing the affinity for the Lysosomal integral membrane protein-2 (LIMP-2) transporter.
Notoginsenoside R1 Attenuates Cisplatin-Induced Ototoxicity by Inducing Heme Oxygenase-1 Expression and Suppressing Oxidative Stress
Authors: Lin Yi-Chun , Ho Yi-Jung , Lin Yuan-Yung , Liao Ai-Ho , Kuo Chao-Yin , Chen Hang-Kang , Chen Hsin-Chien , Wang Chih-Hung , Shih Cheng-Ping
Published: October 2024

Cisplatin-induced ototoxicity occurs in approximately half of patients treated with cisplatin, and pediatric patients are more likely to be affected than adults. The oxidative stress elicited by cisplatin is a key contributor to the pathogenesis of ototoxicity. Notoginsenoside R1 (NGR1), the main bioactive compound of Panax notoginseng saponins, has antioxidant and antiapoptotic effects. This study investigated the ability of NGR1 to protect against cisplatin-induced damage in auditory HEI-OC1 cells and neonatal murine cochlear explants. The viability of HEI-OC1 cells treated with NGR1 and cisplatin was greater than that of cells treated with cisplatin alone. The results of Western blots and immunostaining for cleaved caspase-3 revealed that the level of cleaved caspase-3 in the cells treated with cisplatin was repressed by NGR1. NGR1 attenuated cisplatin-induced cytotoxicity in HEI-OC1 cells. Intracellular reactive oxygen species (ROS) were detected with a DCFDA assay and immunostaining for 4-HNE. The result revealed that its expression was induced by cisplatin and was significantly reduced by NGR1. Moreover, NGR1 can promote heme oxygenase-1 (HO-1) expression at both the mRNA and protein levels. ZNPPIX, an HO-1 inhibitor, was administered to cisplatin-treated cells to investigate the role of HO-1 in the protective effect of NGR1. The suppression of HO-1 activity by ZNPPIX markedly abolished the protective effect of NGR1 on cisplatin-treated cells. Therefore, NGR1 protects cells from cisplatin-induced damage by activating HO-1 and its antioxidative activity. In cochlear explants, NGR1 protects cochlear hair cells and attenuates cisplatin-induced ototoxicity by inhibiting ROS generation. In the group treated with cisplatin alone, prominent loss of outer hair cells and severe damage to the structure of the stereociliary bundles of inner and outer hair cells were observed. Compared with the group treated with cisplatin alone, less loss of outer hair cells (p = 0.009) and better preservation of the stereociliary bundles of hair cells were observed in the group treated with cisplatin and NGR1. In conclusion, these findings indicate that NGR1 can protect against cisplatin-induced ototoxicity by inducing HO-1 expression and suppressing oxidative stress.
Modification and Functionalization of Separators for High Performance Lithium–Sulfur Batteries
Authors: Shen Mengyu , Xu Songshi , Wang Xiuyu , Zhang Yonghui , Feng Yu , Xing Fei , Yang Yingying , Gao Qiqian
Published: October 2024

Lithium–sulfur batteries (LSB) have been recognized as a prominent potential next-generation energy storage system, owing to their substantial theoretical specific capacity (1675 mAh g−1) and high energy density (2600 Wh kg−1). In addition, sulfur’s abundance, low cost, and environmental friendliness make commercializing LSB feasible. However, challenges such as poor cycling stability and reduced capacity, stemming from the formation and diffusion of lithium polysulfides (LiPSs), hinder LSB’s practical application. Introducing functional separators represents an effective strategy to surmount these obstacles and enhance the electrochemical performance of LSBs. Here, we have conducted a comprehensive review of recent advancements in functional separators for LSBs about various (i) carbon and metal compound materials, (ii) polymer materials, and (iii) novel separators in recent years. The detailed preparation process, morphology and performance characterization, and advantages and disadvantages are summarized, aiming to fundamentally understand the mechanisms of improving battery performance. Additionally, the development potential and future prospects of advanced separators are also discussed.
Novel Expression of Apical Bile Acid Transport (ASBT) More Proximally Than Distal Ileum Contributing to Enhanced Intestinal Bile Acid Absorption in Obesity
Authors: Sundaram Shanmuga , Jagadeesan Arunkumar , Paulraj Raja Singh , Sundaram Uma , Arthur Subha
Published: October 2024

Dietary lipid absorption is facilitated by bile acids. In the Zucker rat (ZR) model of obesity, bile acid absorption, mediated by the apical sodium bile acid transporter (ASBT), was increased in villus cells from the distal ileum. However, whether ASBT may be de novo expressed more proximally in the small intestine during obesity to facilitate additional bile acid absorption is not known. For this, starting from the end of the ileum to the mid jejunum, caudal-orally, five intestinal segments of equal length (S1–S5) were separated from lean and obese ZRs (LZR and OZR). Intestinal mucosa obtained from these segments were used for total RNA extraction, RT-qPCR and 3H-TCA uptake. The results showed that bile acid absorption along with the mRNA expression of ASBT and FXR progressively decreased caudal-orally in both LZRs and OZRs but was significantly higher in all small intestinal segments in OZRs. The expression of GATA4 was absent in the distal ileum (S1) in both LZRs and OZRs, but steadily increased along the proximal length in both. However, this steady increase was significantly reduced in the comparative obese proximal intestinal segments S2, S3, S4 and S5. The expressions of bile acid-activated G-protein-coupled bile acid receptor TGR5 and S1PR2 were unaltered in segments S1–S4 but were significantly increased in OZR S5. The paradigm changing observation of this study is that ASBT is expressed more proximally in the small intestine in obesity. This likely increases overall bile acid absorption and thereby lipid absorption in the proximal small intestine in obesity.
Integrated Analysis of Metabolome and Transcriptome Reveals the Effect of Burdock Fructooligosaccharide on the Quality of Chinese Cabbage (Brassica rapa L. ssp. Pekinensis)
Authors: Fu Xin , Wang Lixia , Liu Chenwen , Liu Yuxiang , Li Xiaolong , Yao Tiantian , Jiao Jian , Shu Rui , Li Jingjuan , Zhang Yihui , Wang Fengde , Gao Jianwei
Published: October 2024

Burdock fructooligosaccharide (BFO) is fructose with a low polymerization degree, which could improve the immunity to pathogens, quality, and stress resistance of vegetables. Still, there are no studies on applying BFO in Chinese cabbage. In this study, the effects of exogenous BFO sprayed with different concentrations (0, 5, 10, 20, 30 g·L−1) on the growth and soluble sugar content of Chinese cabbage seedlings were determined. The result showed that 10 g·L−1 was the appropriate spraying concentration. Based on metabolome analysis, a total of 220 differentially accumulated metabolites (DAMs) were found, among which flavonoid metabolites, glucosinolate metabolites, and soluble sugar-related metabolites were the key metabolites involved in improving the quality of Chinese cabbage caused by BFO. Further combination analysis with transcriptome, trans-cinnamate 4-monooxygenase (CYP73A5), and chalcone synthase 1 (CHS1) were more closely associated with the DAMs of flavonoid biosynthesis. Sulfotransferases 18 (SOT18), Branched-chain amino acid amino transferases 6 (BCAT6), and cytochrome P450 monooxygenase (CYP83A1) were the key genes in glucosinolate biosynthesis. Hexokinase (HxK1), beta-glucosidase 8 (BGL08), invertase 3 (INV3), beta-glucosidase 3B (BGL3B), and sucrose phosphate synthase 1 (SPS1) were significantly upregulated, potentially playing crucial roles in the soluble sugar metabolism. In conclusion, these results provided an understanding of the effects of BFO on the expression of genes and the accumulation of metabolites related to quality formation in Chinese cabbage.
Dilated Cardiomyopathy: A Genetic Journey from Past to Future
Authors: Newman Noah A. , Burke Michael A.
Published: October 2024

Dilated cardiomyopathy (DCM) is characterized by reduced systolic function and cardiac dilation. Cases without an identified secondary cause are classified as idiopathic dilated cardiomyopathy (IDC). Over the last 35 years, many cases of IDC have increasingly been recognized to be genetic in etiology with a core set of definitively causal genes in up to 40% of cases. While over 200 genes have been associated with DCM, the evidence supporting pathogenicity for most remains limited. Further, rapid advances in sequencing and bioinformatics have recently revealed a complex genetic spectrum ranging from monogenic to polygenic in DCM. These advances have also led to the discovery of causal and modifier genetic variants in secondary forms of DCM (e.g., alcohol-induced cardiomyopathy). Current guidelines recommend genetic counseling and screening, as well as endorsing a handful of genotype-specific therapies (e.g., device placement in LMNA cardiomyopathy). The future of genetics in DCM will likely involve polygenic risk scores, direct-to-consumer testing, and pharmacogenetics, requiring providers to have a thorough understanding of this rapidly developing field. Herein we outline three decades of genetics in DCM, summarize recent advances, and project possible future avenues for the field.