bluuebunny/crossref_metadata_2025 huggingface dataset:
A Message from the Human Placenta: Structural and Immunomodulatory Defense against SARS-CoV-2
Authors: Kreis Nina-Naomi , Ritter Andreas , Louwen Frank , Yuan Juping
Published: July 2020

The outbreak of the coronavirus disease 2019 (COVID-19) pandemic has caused a global public health crisis. Viral infections may predispose pregnant women to a higher rate of pregnancy complications, including preterm births, miscarriage and stillbirth. Despite reports of neonatal COVID-19, definitive proof of vertical transmission is still lacking. In this review, we summarize studies regarding the potential evidence for transplacental transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), characterize the expression of its receptors and proteases, describe the placental pathology and analyze virus-host interactions at the maternal-fetal interface. We focus on the syncytium, the barrier between mother and fetus, and describe in detail its physical and structural defense against viral infections. We further discuss the potential molecular mechanisms, whereby the placenta serves as a defense front against pathogens by regulating the interferon type III signaling, microRNA-triggered autophagy and the nuclear factor-κB pathway. Based on these data, we conclude that vertical transmission may occur but rare, ascribed to the potent physical barrier, the fine-regulated placental immune defense and modulation strategies. Particularly, immunomodulatory mechanisms employed by the placenta may mitigate violent immune response, maybe soften cytokine storm tightly associated with severely ill COVID-19 patients, possibly minimizing cell and tissue damages, and potentially reducing SARS-CoV-2 transmission.
Derivation and Characterization of Immortalized Human Muscle Satellite Cell Clones from Muscular Dystrophy Patients and Healthy Individuals
Authors: Massenet Jimmy , Gitiaux Cyril , Magnan Mélanie , Cuvellier Sylvain , Hubas Arnaud , Nusbaum Patrick , Dilworth F Jeffrey , Desguerre Isabelle , Chazaud Bénédicte
Published: July 2020

In Duchenne muscular dystrophy (DMD) patients, absence of dystrophin causes muscle wasting by impacting both the myofiber integrity and the properties of muscle stem cells (MuSCs). Investigation of DMD encompasses the use of MuSCs issued from human skeletal muscle. However, DMD-derived MuSC usage is restricted by the limited number of divisions that human MuSCs can undertake in vitro before losing their myogenic characteristics and by the scarcity of human material available from DMD muscle. To overcome these limitations, immortalization of MuSCs appears as a strategy. Here, we used CDK4/hTERT expression in primary MuSCs and we derived MuSC clones from a series of clinically and genetically characterized patients, including eight DMD patients with various mutations, four congenital muscular dystrophies and three age-matched control muscles. Immortalized cultures were sorted into single cells and expanded as clones into homogeneous populations. Myogenic characteristics and differentiation potential were tested for each clone. Finally, we screened various promoters to identify the preferred gene regulatory unit that should be used to ensure stable expression in the human MuSC clones. The 38 clonal immortalized myogenic cell clones provide a large collection of controls and DMD clones with various genetic defects and are available to the academic community.
ROS Dependent Wnt/β-Catenin Pathway and Its Regulation on Defined Micro-Pillars—A Combined In Vitro and In Silico Study
Authors: Staehlke Susanne , Haack Fiete , Waldner Anna-Christin , Koczan Dirk , Moerke Caroline , Mueller Petra , Uhrmacher Adelinde M. , Nebe J. Barbara
Published: July 2020

The physico-chemical surface design of implants influences the surrounding cells. Osteoblasts on sharp-edged micro-topographies revealed an impaired cell phenotype, function and Ca2+ mobilization. The influence of edges and ridges on the Wnt/β-catenin pathway in combination with the cells’ stress response has not been clear. Therefore, MG-63 osteoblasts were studied on defined titanium-coated micro-pillars (5 × 5 × 5 µm) in vitro and in silico. MG-63s on micro-pillars indicated an activated state of the Wnt/β-catenin pathway. The β-catenin protein accumulated in the cytosol and translocated into the nucleus. Gene profiling indicated an antagonism mechanism of the transcriptional activity of β-catenin due to an increased expression of inhibitors like ICAT (inhibitor of β-catenin and transcription factor-4). Cells on pillars produced a significant reactive oxygen species (ROS) amount after 1 and 24 h. In silico analyses provided a detailed view on how transcriptional activity of Wnt signaling is coordinated in response to the oxidative stress induced by the micro-topography. Based on a coordinated expression of regulatory elements of the Wnt/β-catenin pathway, MG-63s are able to cope with an increased accumulation of β-catenin on micro-pillars and suppress an unintended target gene expression. Further, β-catenin may be diverted into other signaling pathways to support defense mechanisms against ROS.
Corrosion Behavior and Mechanism of Carbon Ion-Implanted Magnesium Alloy
Authors: Yu Banglong , Dai Jun , Ruan Qingdong , Liu Zili , Chu Paul K.
Published: July 2020

Carbon ion implantation was conducted on an AM60 magnesium alloy with fluences between 1 × 1016 and 6 × 1016 ions/cm2 and an energy of 35 keV. The microstructure and electrochemical properties of the samples were systematically characterized by X-ray photoelectron spectroscopy, X-ray diffraction, Raman scattering, scanning electron microscopy, transmission electron microscopy, and electrochemical methods. These studies reveal that a 250 nm-thick C-rich layer is formed on the surface and the Mg2C3 phase embeds in the ion-implanted region. The crystal structure of the Mg2C3 was constructed, and an electronic density map was calculated by density-functional theory calculation. The large peak in the density of states (DOS) shows two atomic p orbitals for Mg2C3. The main electron energy is concentrated between −50 and −40 eV, and the electron energy mainly comes from Mg (p) and Mg (s). The electrochemical experiments reveal that the Ecorr is −1.35 V and Icorr is 20.1 μA/cm2 for the sample implanted with the optimal fluence of 6 × 1016 ions/cm2. The sample from C ion implantation gives rise to better corrosion resistance.
A Proposed Procedure for Discriminating between Nasal Secretion and Saliva by RT-qPCR
Authors: Akutsu Tomoko , Watanabe Ken
Published: July 2020

In forensic casework, nasal secretion can be a good source of DNA. Moreover, saliva can prove useful in cases of sexual assault. However, discriminating between these body fluids is often difficult because of cross-reactivity between them on presumptive and confirmatory tests. Therefore, an RT-qPCR procedure was developed to discriminate between nasal secretion and saliva. Characteristic genes in nasal secretion and/or saliva (BPIFA1, STATH, HTN3, and PRH2) were selected as candidates. Discrimination criteria were established based on the expression levels of these markers in various body fluids. In addition, a flowchart was proposed and used to discriminate among nasal secretion, saliva, and other body fluids in various forensic samples. BPIFA1 was highly expressed in nasal secretion but was also expressed in saliva, semen, and vaginal fluid at trace levels. STATH was expressed in nasal secretion and saliva but not in other body fluids. HTN3 was specifically expressed in most of the saliva samples, as reported previously. Unexpectedly, PRH2 was expressed in only a few saliva samples. Using the proposed criteria and flowchart, nasal secretion and saliva were successfully discriminated among the various body fluids tested. The developed procedure could be useful in forensic casework.
Mobile Applications for Assessing Human Posture: A Systematic Literature Review
Authors: Moreira Rayele , Teles Ariel , Fialho Renan , Baluz Rodrigo , Santos Thalyta Cibele , Goulart-Filho Rômulo , Rocha Laiane , Silva Francisco José , Gupta Nishu , Bastos Victor Hugo , Teixeira Silmar
Published: July 2020

Smartphones are increasingly incorporated with features such as sensors and high resolution cameras that empower their capabilities, enabling their use for varied activities including human posture assessments. Previous reviews have discussed methods used in postural assessment but none of them focused exclusively on mobile applications. This paper systematically reviews mobile applications proposed for analyzing human posture based on alignment of the body in the sagittal and coronal plane. The main digital libraries were searched, 26 articles published between 2010 and 2020 were selected, and 13 mobile applications were identified, classified and discussed. Results showed that the use of mobile applications to assist with posture assessment have been demonstrated to be reliable, and this can contribute to clinical practice of health professionals, especially the assessment and reassessment phases of treatments, despite some variations when compared to traditional methods. Moreover, in the case of image-based applications, we highlight the advantage that measurements can be taken with the assessor at a certain distance with respect to the patient’s position, which is an important function for assessments performed in pandemic times such as the outbreak of COVID-19.
D-Band Frequency Tripler Module Using Anti-Parallel Diode Pair and Waveguide Transitions
Authors: Doo Jihoon , Kim Jongyoun , Jeong Jinho
Published: July 2020

In this paper, D-band (110–170 GHz) frequency tripler module is presented using anti-parallel GaAs Schottky diode pair and waveguide-to-microstrip transitions. The anti-parallel diode pair is used as a nonlinear device generating harmonic components for Q-band input signal (33–50 GHz). The diode is zero-biased to eliminate the bias circuits and thus minimize the number of circuit components for low-cost hybrid fabrication. The anti-parallel connection of two identical diodes effectively suppresses DC and even harmonics in the output. Furthermore, the first and second harmonics of Q-band input signal are cut off by D-band rectangular waveguide. Input and output impedance matching networks are designed based on the optimum impedances determined by harmonic source- and load-pull simulations using the developed nonlinear diode model. Waveguide-to-microstrip transitions at Q- and D-bands are also designed using E-plane probe to package the frequency tripler in the waveguide module. The compensation circuit is added to reduce the impedance mismatches by bond-wires connecting two separate substrates. The fabricated frequency tripler module produces a maximum output power of 5.4 dBm at 123 GHz under input power of 20.5 dBm. A 3 dB bandwidth is as wide as 22.5% from 118.5 to 148.5 GHz at the input power of 15.0 dBm. This result corresponds to the excellent bandwidth performance with a conversion gain comparable to the previously reported frequency tripler operating at D-band.
Wind Loading on Scaled Down Fractal Tree Models of Major Urban Tree Species in Singapore
Authors: Chan Woei-Leong , Eng Yong , Ge Zhengwei , Lim Chi Wan Calvin , Gobeawan Like , Poh Hee Joo , Wise Daniel Joseph , Burcham Daniel C. , Lee Daryl , Cui Yongdong , Khoo Boo Cheong
Published: July 2020

Estimation of the aerodynamic load on trees is essential for urban tree management to mitigate the risk of tree failure. To assess that in a cost-effective way, scaled down tree models and numerical simulations were utilized. Scaled down tree models reduce the cost of experimental studies and allow the studies to be conducted in a controlled environment, namely in a wind or water tunnel, but the major challenge is to construct a tree model that resembles the real tree. We constructed 3D-printed scaled down fractal tree models of major urban tree species in Singapore using procedural modelling, based on species-specific growth processes and field statistical data gathered through laser scanning of real trees. The tree crowns were modelled to match the optical porosity of real trees. We developed a methodology to model the tree crowns using porous volumes filled with randomized tetrahedral elements. The wind loads acting on the tree models were then measured in the wind tunnel and the velocity profiles from selected models were captured using particle image velocimetry (PIV). The data was then used for the validation of Large Eddy Simulations (LES), in which the trees were modelled via a discretized momentum sink with 10–20 elements in width, height, and depth, respectively. It is observed that the velocity profiles and drag of the simulations and the wind tunnel tests are in reasonable agreement. We hence established a clear relationship between the measured bulk drag on the tree models in the wind tunnel, and the local drag coefficients of the discretized elements in the simulations. Analysis on the bulk drag coefficient also shows that the effect of complex crown shape could be more dominant compared to the frontal optical porosity.
Stand-Level Components of a Growth and Yield Model for Nothofagus Mixed Forests from Southern Chile
Authors: Palmas Sebastian , Moreno Paulo C. , Cropper Wendel P. , Ortega Alicia , Gezan Salvador A.
Published: July 2020

Reliable information on stand dynamics and development is needed to improve management decisions on mixed forests, and essential tools for this purpose are forest growth and yield (G&Y) models. In this study, stand-level G&Y models were built for cohorts within the natural mixed second-growth Nothofagus-dominated forests in Chile. All currently available (but limited) data, consisting of a series of stratified temporary and permanent plots established in the complete range of this forest type, were used to fit and validate these models. Linear and nonlinear models were considered, where dominant stand age, number of trees, and the proportion of basal area of Nothofagus species resulted in significant predictors to project future values of stand basal area for the different cohorts (with R2 > 0.51 for the validation datasets). Mortality was successfully modeled (R2 = 0.79), based on a small set of permanent plots, using the concept of self-thinning with a proposed model defined by the idea that, as stands get closer to a maximum density, they experience higher levels of mortality. The evaluation of these models indicated that they adequately represent the current understanding of dynamics of basal area and mortality of Nothofagus and companion species in these forests. These are the first models fitted over a large geographical area that consider the dynamics of these mixed forests. It is suggested that the proposed models should constitute the main components of future implementations of G&Y model systems.
Next Generation Sequencing of 134 Children with Autism Spectrum Disorder and Regression
Authors: Yin Jiani , Chun Chun-An , Zavadenko Nikolay N. , Pechatnikova Natalia L. , Naumova Oxana Yu. , Doddapaneni Harsha V. , Hu Jianhong , Muzny Donna M. , Schaaf Christian P. , Grigorenko Elena L.
Published: July 2020

Approximately 30% of individuals with autism spectrum disorder (ASD) experience developmental regression, the etiology of which remains largely unknown. We performed a complete literature search and identified 47 genes that had been implicated in such cases. We sequenced these genes in a preselected cohort of 134 individuals with regressive autism. In total, 16 variants in 12 genes with evidence supportive of pathogenicity were identified. They were classified as variants of uncertain significance based on ACMG standards and guidelines. Among these were recurring variants in GRIN2A and PLXNB2, variants in genes that were linked to syndromic forms of ASD (GRIN2A, MECP2, CDKL5, SCN1A,PCDH19, UBE3A, and SLC9A6), and variants in the form of oligogenic heterozygosity (EHMT1, SLC9A6, and MFSD8).
Knee Osteoarthritis: A Review of Pathogenesis and State-Of-The-Art Non-Operative Therapeutic Considerations
Authors: Primorac Dragan , Molnar Vilim , Rod Eduard , Jeleč Željko , Čukelj Fabijan , Matišić Vid , Vrdoljak Trpimir , Hudetz Damir , Hajsok Hana , Borić Igor
Published: July 2020

Being the most common musculoskeletal progressive condition, osteoarthritis is an interesting target for research. It is estimated that the prevalence of knee osteoarthritis (OA) among adults 60 years of age or older is approximately 10% in men and 13% in women, making knee OA one of the leading causes of disability in elderly population. Today, we know that osteoarthritis is not a disease characterized by loss of cartilage due to mechanical loading only, but a condition that affects all of the tissues in the joint, causing detectable changes in tissue architecture, its metabolism and function. All of these changes are mediated by a complex and not yet fully researched interplay of proinflammatory and anti-inflammatory cytokines, chemokines, growth factors and adipokines, all of which can be measured in the serum, synovium and histological samples, potentially serving as biomarkers of disease stage and progression. Another key aspect of disease progression is the epigenome that regulates all the genetic expression through DNA methylation, histone modifications, and mRNA interference. A lot of work has been put into developing non-surgical treatment options to slow down the natural course of osteoarthritis to postpone, or maybe even replace extensive surgeries such as total knee arthroplasty. At the moment, biological treatments such as platelet-rich plasma, bone marrow mesenchymal stem cells and autologous microfragmented adipose tissue containing stromal vascular fraction are ordinarily used. Furthermore, the latter two mentioned cell-based treatment options seem to be the only methods so far that increase the quality of cartilage in osteoarthritis patients. Yet, in the future, gene therapy could potentially become an option for orthopedic patients. In the following review, we summarized all of the latest and most important research in basic sciences, pathogenesis, and non-operative treatment.
Stabilization of Landslides Sliding Layer Using Electrokinetic Phenomena and Vacuum Treatment
Authors: Ukleja Janusz
Published: July 2020

The article presents the efficiency of application of cohesive soil dewatering for increasing its resistance to shearing, which influences the mass stability of flysch rock. Studies of the typical soil constituting the contact layer initiating the sliding of existing Carpathian flysch landslides were conducted. This aspect was examined because the water content of this soil decides its ability to form a sliding surface of the landslide block soil. The soil was subjected to changes in water content by dewatering with different methods. The influence of dewatering by self-acting gravitational outflow was examined and was additionally aided by two selected methods: electrokinetic phenomena and vacuum treatment. The model study conducted demonstrates the influence of the abovementioned dewatering methods on increasing the strength parameter of the soil at the contact layer in which sliding surfaces can be created. The paper also demonstrates the degree to which the application of the vacuum and electrokinetic treatment caused by DC current voltage influences the draining, decrease of plasticity, and increase of soil shear stress resistance. The application conditions and increase in effectiveness due to the application of the studied methods were determined. The proposed methods allowed for the strengthening of slopes for two exemplary landslides which formed in the area of occurrence of the Carpathian flysch.
Assessing the Performance of Different Time of Concentration Equations in Urban Ungauged Watersheds: Case Study of Cartagena de Indias, Colombia
Authors: González-Álvarez Álvaro , Molina-Pérez José , Meza-Zúñiga Brandon , Viloria-Marimón Orlando M. , Tesfagiorgis Kibrewossen , Mouthón-Bello Javier A.
Published: July 2020

In ungauged watersheds, the estimation of the time of concentration (Tc) is always a challenging task due to the intrinsic uncertainty involved when making assumptions. Given that Tc is one of the main inputs in a hydrological analysis for the design of hydraulic structures for stormwater management, ten equations (including one proposed in several local studies) and two Tc methodologies (overland flow time plus channel flow time) were used to compute the Tc in fifteen urban ungauged watersheds, located in Cartagena de Indias (Colombia), with different area sizes and slopes to statistically assess their performance against the value obtained via the Natural Resources Conservation Service (NRCS) velocity method (assumed to be the true value). According to the Nash–Sutcliffe efficiency index, none of the equations proved to be reliable in all watersheds as only four equations predicted the Tc value in 53% of the cases. In addition, based on the percent bias, all equations tended to significantly over- or underestimate the Tc, which affects the quantification of the runoff volume necessary for, among others, the implementation of best management practices for watershed management (e.g., conventional and/or sustainable drainage system design), flood-prone area delineation and flood risk analyses, urban planning, and stream restoration.
Parents’ and Peers’ Autonomy Support and Exercise Intention for Adolescents: Integrating Social Factors from the Self-Determination Theory and the Theory of Planned Behaviour
Authors: Sicilia Alvaro , Águila Cornelio , Posse Magalí , Alcaraz-Ibáñez Manuel
Published: July 2020

Based on the theory of planned behaviour and self-determination theory, the objective of the present study was to analyse the relationship between (i) parents’ and peers’ autonomy support, and (ii) exercise intention in adolescents, while also considering the mediating role of attitude, control, subjective norms, and descriptive norms. A total of 428 secondary school students, aged between 13 and 19 years old (Mage = 15.30, SD = 1.15), filled in a questionnaire assessing the variables of interest. The relationships between the study variables were examined through a mediation model with bootstrapping technique (20,000 samples) using Mplus v. 7 software. The results showed that the perception of parents’ autonomy support was positively and statistically significant associated with exercise intention; this occurring indirectly through attitude and control both in boys and girls, as well as through subjective norms in the case of girls. Conversely, the perception of peers’ autonomy support was positively and statistically significant associated with exercise intention; this occurring directly both in boys and girls, as well as indirectly through attitude in the case of girls. These findings suggest that, by involving a form of pressure (i.e., subjective/descriptive norms), perceptions of autonomy support may play a more important role than other forms of social influence in predicting exercise intention in adolescents.
Effects of Temperature on Lifespan of Drosophila melanogaster from Different Genetic Backgrounds: Links between Metabolic Rate and Longevity
Authors: Mołoń Mateusz , Dampc Jan , Kula-Maximenko Monika , Zebrowski Jacek , Mołoń Agnieszka , Dobler Ralph , Durak Roma , Skoczowski Andrzej
Published: July 2020

Despite many studies of the aging process, questions about key factors ensuring longevity have not yet found clear answers. Temperature seems to be one of the most important factors regulating lifespan. However, the genetic background may also play a key role in determining longevity. The aim of this study was to investigate the relationship between the temperature, genetic background (fruit fly origin), and metabolic rate on lifespan. Experiments were performed with the use of the wild type Drosophila melanogaster fruit flies originating from Australia, Canada, and Benin and the reference OregonR strain. The metabolic rate of D. melanogaster was measured at 20 °C, 25 °C, and 28 °C in an isothermal calorimeter. We found a strong negative relationship between the total heat flow and longevity. A high metabolic rate leads to increased aging in males and females in all strains. Furthermore, our results showed that temperature has a significant effect on fecundity and body weight. We also showed the usefulness of the isothermal calorimetry method to study the effect of environmental stress conditions on the metabolic activity of insects. This may be particularly important for the forecasting of impact of global warming on metabolic activity and lifespan of various insects.
Comparative Analysis of Wind Energy Generation Forecasts in Poland and Portugal and Their Influence on the Electricity Exchange Prices
Authors: Rogus Radomir , Castro Rui , Sołtysik Maciej
Published: July 2020

Currently, the privileged position of wind energy producers is being weakened by their enforced participation in the market on equal terms. This requires accurate production forecasting. The main aim of this study is to comparatively examine the wind generation forecasts in Poland and Portugal, as well as to verify their influence on the day-ahead market prices. The statistical analysis revealed significant deviations of the forecasted and actual wind production in both countries, which referred to the corresponding spot and balancing prices caused considerable financial losses by the wind energy suppliers. In this paper, the influence of the wind generation forecasts on the spot prices has been examined through developed the auto-regressive moving average (ARMA), ARMA with exogenous inputs (ARMAX) and non-linear auto-regressive neural network (NAR), NAR with exogenous inputs (NARX)artificial neural network (ANN) models. The results have shown that the usability of the information of forecasted wind generation is not unequivocal in models developed for spot prices in Poland, mainly because of the randomness and volatility of recorded wind generation forecasts. However, in the case of Portugal, the forecasted wind generation occurred to be a valuable input in spot prices models, which results in an improvement in the models’ accuracy.
Mentally Sick or Not—(Bio)Markers of Psychiatric Disorders Needed
Authors: Waszkiewicz Napoleon
Published: July 2020

Psychiatric disorders, also called mental illnesses or mental disorders, constitute a wide group of disorders including major depression disorder (MDD), bipolar disorder (BD), schizophrenia (SCZ) and other psychoses, anxiety disorders (ANX), substance-related disorders (SRD), dementia, developmental disorders e [...]
Volume of Lateral Geniculate Nucleus in Patients with Glaucoma in 7Tesla MRI
Authors: Kosior-Jarecka Ewa , Pankowska Anna , Polit Piotr , Stępniewski Andrzej , Symms Mark Roger , Kozioł Paulina , Żarnowski Tomasz , Pietura Radosław
Published: July 2020

The aim of the study was to assess the volume of the lateral geniculate nucleus (LGN) in patients with open-angle glaucoma in 7Tesla MRI and to evaluate its relation to RNFL thickness and VF indices. Material and methods. The studied group consisted of 20 open-angle glaucoma patients with bilaterally the same stage of glaucoma (11 with early glaucoma and nine with advanced glaucoma) and nine healthy volunteers from the Department of Diagnostics and Microsurgery of Glaucoma, Medical University of Lublin, Poland. Circumpapillary RNFL-thickness measurements were performed using OCT in all patients and visual fields were performed in the glaucoma group. A 7Tesla MRI was performed to assess the volume of both lateral geniculate bodies. Results. The LGN volume varied significantly between groups from 122.1 ± 14.4 mm3 (right LGN) and 101.6 ± 13.3 mm3 (left LGN) in the control group to 80.2 ± 17.7 mm3 (right LGN) and 71.8 ± 14.2 mm3 (left LGN) in the advanced glaucoma group (right LGN p = 0.003, left LGN p = 0.018). However, volume values from early glaucoma: right LGN = 120.2 ± 26.5 mm3 and left LGN = 103.2 ± 28.0 mm3 differed significantly only from values from the advanced group (right LGN p = 0.006, left LGN p = 0.012), but not from controls (right LGN p = 0.998, left LGN p = 0.986). There were no significant correlations between visual field indices (MD (mean deviation) and VFI (visual field index)) and LGN volumes in both glaucoma groups. Significant correlations between mean RNFL (retinal nerve fiber layers) thickness and corresponding and contralateral LGN were observed for the control group (corresponding LGN: p = 0.064; contralateral LGN: p = 0.031) and early glaucoma (corresponding LGN: p = 0.017; contralateral LGN: p = 0.008), but not advanced glaucoma (corresponding LGN: p = 0.496; contralateral LGN: p = 0.258). Conclusions. The LGN volume decreases in the course of glaucoma. These changes are correlated with RNFL thickness in early stages of glaucoma and are not correlated with visual field indices.
NIR Photodynamic Destruction of PDAC and HNSCC Nodules Using Triple-Receptor-Targeted Photoimmuno-Nanoconjugates: Targeting Heterogeneity in Cancer
Authors: Bano Shazia , Obaid Girgis , Swain Joseph W. R. , Yamada Marina , Pogue Brian W. , Wang Kenneth , Hasan Tayyaba
Published: July 2020

Receptor heterogeneity in cancer is a major limitation of molecular targeting for cancer therapeutics. Single-receptor-targeted treatment exerts selection pressures that result in treatment escape for low-receptor-expressing tumor subpopulations. To overcome this potential for heterogeneity-driven resistance to molecular targeted photodynamic therapy (PDT), we present for the first time a triple-receptor-targeted photoimmuno-nanoconjugate (TR-PIN) platform. TR-PIN functionalization with cetuximab, holo-transferrin, and trastuzumab conferred specificity for epidermal growth factor receptor (EGFR), transferrin receptor (TfR), and human epidermal growth factor receptor 2 (HER-2), respectively. The TR-PINs exhibited up to a 24-fold improvement in cancer cell binding compared with EGFR-specific cetuximab-targeted PINs (Cet-PINs) in low-EGFR-expressing cell lines. Photodestruction using TR-PINs was significantly higher than the monotargeted Cet-PINs in heterocellular 3D in vitro models of heterogeneous pancreatic ductal adenocarcinoma (PDAC; MIA PaCa-2 cells) and heterogeneous head and neck squamous cell carcinoma (HNSCC, SCC9 cells) containing low-EGFR-expressing T47D (high TfR) or SKOV-3 (high HER-2) cells. Through their capacity for multiple tumor target recognition, TR-PINs can serve as a unique and amenable platform for the effective photodynamic eradication of diverse tumor subpopulations in heterogeneous cancers to mitigate escape for more complete and durable treatment responses.
Tackling the Burden of Osteoarthritis as a Health Care Opportunity in Indigenous Communities—A Call to Action
Authors: O’Brien Penny , Bunzli Samantha , Lin Ivan , Gunatillake Tilini , Bessarab Dawn , Coffin Juli , Garvey Gail , Dowsey Michelle , Choong Peter
Published: July 2020

Osteoarthritis is a highly prevalent and disabling disease, causing a significant individual and socioeconomic burden worldwide. Until now, there has been a dearth of research exploring the impact of osteoarthritis in global Indigenous communities. Osteoarthritis has a similar risk factor profile to many chronic diseases that disproportionately affect Indigenous peoples. In this editorial, we argue that osteoarthritis and associated mobility restrictions play a central role in the chronic disease profile of Indigenous peoples. We present a call to action for clinicians and health care providers, researchers and policymakers to begin to recognise the interrelated nature of osteoarthritis and chronic disease. We have an opportunity to change the way we do business, to improve access to culturally secure osteoarthritis care and the health and wellbeing of Indigenous communities.
Correlations between Iron Metabolism Parameters, Inflammatory Markers and Lipid Profile Indicators in Patients with Type 1 and Type 2 Diabetes Mellitus
Authors: N. Musina Nadezhda , V. Saprina Tatiana , S. Prokhorenko Tatiana , Kanev Alexander , P. Zima Anastasia
Published: July 2020

This study aims to establish relationships between inflammatory status, ferrokinetics and lipid metabolism in patients with diabetes mellitus. Subclinical inflammation was assessed by levels of high-sensitive C-reactive protein, tumor necrosis factor-α and erythrocyte sedimentation rate. Iron metabolism parameters included complete blood count, serum iron, transferrin and ferritin. Metabolic status assessment included lipid profile, glycated hemoglobin and microalbuminuria measurement. As a result of the study it was possible to establish both general (universal) and diabetes mellitus (DM) type-dependent relationships between the parameters of lipid profile and metabolic control in DM. High-density lipoprotein cholesterol (HDL-C) levels negatively correlated with microalbuminuria (r = −0.293; p ˂ 0.05 for type 1 diabetes and r = −0.272; p ˂ 0.05 for type 2 diabetes). Ferritin concentration positively correlated with triglyceride level (r = 0.346; p ˂ 0.05 for type 1 diabetes and r = 0.244; p ˂ 0.05 for type 2 diabetes). In type 1 diabetes, a negative correlation was discovered between estimated glomerular filtration rate (eGFR) and LDL-C (r = −0.480; p ˂ 0.05), very low-density-lipoprotein cholesterol (VLDL-C) (r = −0.490; p ˂ 0.05) and triglycerides (r = −0.553; p ˂ 0.05), and a positive one between C-reactive protein concentration and triglyceride level (r = 0.567; p ˂ 0.05). Discovered relationships between lipid profile indices, inflammatory status and microalbuminuria confirmed mutual influence of hyperlipidemia, inflammation and nephropathy in diabetes patients. Obtained results justify the strategy of early hypolipidemic therapy in patients with diabetes mellitus to prevent the development and progression of microvascular complications.
Relative Biological Effectiveness of Carbon Ions for Head-and-Neck Squamous Cell Carcinomas According to Human Papillomavirus Status
Authors: Osu Naoto , Kobayashi Daijiro , Shirai Katsuyuki , Musha Atsushi , Sato Hiro , Hirota Yuka , Shibata Atsushi , Oike Takahiro , Ohno Tatsuya
Published: July 2020

Carbon-ion radiotherapy (CIRT) has strong antitumor effects and excellent dose conformity. In head-and-neck squamous cell carcinoma (HNSCC), human papillomavirus (HPV) status is a prognostic factor for photon radiotherapy outcomes. However, the effect of HPV status on the sensitivity of HNSCCs to carbon ions remains unclear. Here, we showed that the relative biological effectiveness (RBE) of carbon ions over X-rays was higher in HPV-negative cells than in HSGc-C5 cells, which are used for CIRT dose establishment, whereas the RBE in HPV-positive cells was modest. These data indicate that CIRT is more advantageous in HPV-negative than in HPV-positive HNSCCs.
Parental Selenium Nutrition Affects the One-Carbon Metabolism and the Hepatic DNA Methylation Pattern of Rainbow Trout (Oncorhynchus mykiss) in the Progeny
Authors: Wischhusen Pauline , Saito Takaya , Heraud Cécile , Kaushik Sadasivam J. , Fauconneau Benoit , Antony Jesu Prabhu Philip , Fontagné-Dicharry Stéphanie , Skjærven Kaja H.
Published: July 2020

Selenium is an essential micronutrient and its metabolism is closely linked to the methionine cycle and transsulfuration pathway. The present study evaluated the effect of two different selenium supplements in the diet of rainbow trout (Onchorhynchus mykiss) broodstock on the one-carbon metabolism and the hepatic DNA methylation pattern in the progeny. Offspring of three parental groups of rainbow trout, fed either a control diet (NC, basal Se level: 0.3 mg/kg) or a diet supplemented with sodium selenite (SS, 0.8 mg Se/kg) or hydroxy-selenomethionine (SO, 0.7 mg Se/kg), were collected at swim-up fry stage. Our findings suggest that parental selenium nutrition impacted the methionine cycle with lower free methionine and S-adenosylmethionine (SAM) and higher methionine synthase (mtr) mRNA levels in both selenium-supplemented treatments. DNA methylation profiling by reduced representation bisulfite sequencing (RRBS) identified differentially methylated cytosines (DMCs) in offspring livers. These DMCs were related to 6535 differentially methylated genes in SS:NC, 6890 in SO:NC and 7428 in SO:SS, respectively. Genes with the highest methylation difference relate, among others, to the neuronal or signal transmitting and immune system which represent potential targets for future studies.
Facile Synthesis of Surface-Modified Carbon Quantum Dots (CQDs) for Biosensing and Bioimaging
Authors: Janus Łukasz , Radwan-Pragłowska Julia , Piątkowski Marek , Bogdał Dariusz
Published: July 2020

Recently, fluorescent probes became one of the most efficient tools for biosensing and bioimaging. Special attention is focused on carbon quantum dots (CQDs), which are characterized by the water solubility and lack of cytotoxicity. Moreover, they exhibit higher photostability comparing to traditional organic dyes. Currently, there is a great need for the novel, luminescent nanomaterials with tunable properties enabling fast and effective analysis of the biological samples. In this article, we propose a new, ecofriendly bottom-up synthesis approach for intelligent, surface-modified nanodots preparation using bioproducts as a raw material. Obtained nanomaterials were characterized over their morphology, chemical structure and switchable luminescence. Their possible use as a nanodevice for medicine was investigated. Finally, the products were confirmed to be non-toxic to fibroblasts and capable of cell imaging.
Biomimetic Design for a Dual Concentric Porous Titanium Scaffold with Appropriate Compressive Strength and Cells Affinity
Authors: Lee Han , Liao Jiunn-Der , Guo Yao-Sheng , Juang Yung-Der
Published: July 2020

In repairing or replacing damaged bones, a dual concentric porous titanium scaffold (P-Tix-y) has emerged as a promising bio-mimic design. Herein, various P-Tix-y were made and sintered with relatively dense (x = 10, 20, or 30% porosity) and loose (y = 45, 55, or 65 porosity) structures. Firstly, NaCl was used as the pore-forming additive and followed by a hydrothermal removal method. The compressive strength of the as-formed P-Tix_y and surface morphology, nanomechanical property, and cells’ affinity on the cross-sectioned surface of P-Tix_y (CP-Tix_y) were then characterized. The results demonstrate that the compressive strength of P-Ti10_45, P-Ti20_45, or P-Ti20_55 exhibits a relatively mild decline (e.g., in the range of 181 and 97 MPa, higher than the required value of 70 MPa) and suitable porosities for the intended structure. Nano-hardness on the solid surface of CP-Tix_y shows roughly consistent with that of CP-Ti (i.e., ~8.78 GPa), thus, the porous structure of CP-Tix_y remains mostly unaffected by the addition of NaCl and subsequent sintering process. Most of the surfaces of CP-Tix_y exhibit high fibroblast (L929) cell affinity with low cell mortality. Notably, in the hFOB 1.19 cell adhesion and proliferation test, CP-Ti20_55 and CP-Ti20_65 reveal high cell viability, most probably relating with the assembly of dual porosities with interconnected pores. Overall, the sample P-Ti20_55 provides a relatively load-bearable design with high cell affinity and is thus promising as a three-dimensional bio-scaffold.
Self-Sealing Process Evaluation Method Using Ultrasound Technique in Cement Composites with Mineral Additives
Authors: Tomczak Kamil , Jakubowski Jacek , Kotwica Łukasz
Published: July 2020

The self-sealing process, associated with chemical and microstructural changes inside damaged cement-based composites, leads to the recovery of the original material integrity. Assessing the magnitude of internal changes in samples using non-destructive techniques to capture only the self-sealing effects is difficult. The challenge is evaluating the differences between subsequent observations in time and between samples with different properties. This paper proposes a new approach to the use of an ultrasonic technique for self-sealing investigation. The method allows the quantification of material changes strictly related to self-sealing processes, excluding changes caused by the naturally progressing hydration of binders. The applied ultrasonic pulse velocity (UPV) data processing procedure allows the investigation of material changes inside and near the cracks, the effects of stimulating the self-sealing of cement composites with mineral additives, and the assessment of changes over time. An important aspect of the method is the sample preparation procedure and testing conditions that reduce the impact of moisture content on the UPV measurements. New parameters allowing the quantitative characterization of the self-sealing process are proposed. The method was evaluated using cement mortars modified with siliceous fly ash with induced cracks 0 to 750 µm wide, which were then cured in water for 152 days. The maximum degree of effective crack filling as a result of autogenous self-sealing in the tested mortars was determined to range from 33% to 57%. Observations of the microstructure of the crack surface confirmed that apart from the volume of the newly formed products, the density of these products may have a key impact on the ultrasonic measurements of the self-sealing performance. The studies were supplemented by the examination of the compression strength of mortars, mortar sample scanning and computer image processing, and observations using an optical microscope and scanning electron microscope with energy dispersive spectroscopy.
Transport and Electrochemical Characteristics of CJMCED Homogeneous Cation Exchange Membranes in Sodium Chloride, Calcium Chloride, and Sodium Sulfate Solutions
Authors: Sarapulova Veronika , Pismenskaya Natalia , Butylskii Dmitrii , Titorova Valentina , Wang Yaoming , Xu Tongwen , Zhang Yang , Nikonenko Victor
Published: July 2020

Recently developed and produced by Hefei Chemjoy Polymer Material Co. Ltd., homogeneous CJMC-3 and CJMC-5 cation-exchange membranes (CJMCED) are characterized. The membrane conductivity in NaCl, Na2SO4, and CaCl2 solutions, permeability in respect to the NaCl and CaCl2 diffusion, transport numbers, current–voltage curves (CVC), and the difference in the pH (ΔpH) of the NaCl solution at the desalination compartment output and input are examined for these membranes in comparison with a well-studied commercial Neosepta CMX cation-exchange membrane produced by Astom Corporation, Japan. It is found that the conductivity, CVC (at relatively low voltages), and water splitting rate (characterized by ΔpH) for both CJMCED membranes are rather close to these characteristics for the CMX membrane. However, the diffusion permeability of the CJMCED membranes is significantly higher than that of the CMX membrane. This is due to the essentially more porous structure of the CJMCED membranes; the latter reduces the counterion permselectivity of these membranes, while allowing much easier transport of large ions, such as anthocyanins present in natural dyes of fruit and berry juices. The new membranes are promising for use in electrodialysis demineralization of brackish water and natural food solutions.
Effects of On-Line Vortex Cooling on the Microstructure and Mechanical Properties of Wire Arc Additively Manufactured Al-Mg Alloy
Authors: Wang Baoxing , Yang Guang , Zhou Siyu , Cui Can , Qin Lanyun
Published: July 2020

A novel on-line vortex cooling powered by low-cost compressed air was proposed to reduce common defects such as low forming precision, coarse grains, and pores caused by heat accumulation in the Wire Arc Additive Manufacturing (WAAM) of aluminum alloy. The impacts of interlayer cooling (IC), substrate cooling (SC), on-line cooling (OL), and natural cooling (NC) processes were compared on the morphology, microstructure, and mechanical properties of as-deposited walls, revealing that the OL process significantly lowers the interlayer temperature and improves forming precision. The high cooling rate produced by the OL process reduced the absorption of hydrogen in the molten pool, lowering porosity. Furthermore, the grains are refined due to the developed undercooling. However, the high cooling rate enhanced the segregation potential of Mg element and raised the content of the β phase. Conclusively, the maximum tensile strength, elongation, and microhardness of the as-deposited wall are achieved via the OL process, and the fine-grain strengthening mechanism plays an important role in improving mechanical properties. The OL process is cheaper and poses a significant effect; it is highly suitable for the additive manufacturing of complex components compared with other forced cooling processes.
Erosion–Corrosion of AISI 304L Stainless Steel Affected by Industrial Copper Tailings
Authors: Soliz Álvaro , Cáceres Luis , Pineda Fabiola , Galleguillos Felipe
Published: July 2020

A comprehensive analysis of the erosion–corrosion behavior of AISI 304L stainless steel immersed in 0.5 M NaCl solution with the addition of industrial tailing particles obtained from the copper mining industry is reported. From fundamental studies using combined measurements of potentiodynamic and weight loss techniques, the pure corrosion, pure erosion, and their synergism on the total wear rate were evaluated. The results showed that the presence of soluble chemical reagents dragged by tailing particles significantly affects the pure corrosion rate in comparison with a NaCl solution without these chemical reagents. In addition, the wear of stainless steel by pure erosion was found to be more important than that of pure corrosion. Erosion–corrosion tests performed under an open circuit potential behavior indicate lower and higher wear values than that obtained for erosion and corrosion rates, respectively. Through these results, it was possible to determine an antagonistic effect for AISI 304L stainless steel in industrial tailings slurries. These results are supported by changes in the electrochemical parameters, passive film stability, and morphological attributes.
A Review of Heat Treatments on Improving the Quality and Residual Stresses of the Ti–6Al–4V Parts Produced by Additive Manufacturing
Authors: Teixeira Óscar , Silva Francisco J. G. , Ferreira Luís P. , Atzeni Eleonora
Published: July 2020

Additive manufacturing (AM) can be seen as a disruptive process that builds complex components layer upon layer. Two of its distinct technologies are Selective Laser Melting (SLM) and Electron Beam Melting (EBM), which are powder bed fusion processes that create metallic parts with the aid of a beam source. One of the most studied and manufactured superalloys in metal AM is the Ti–6Al–4V, which can be applied in the aerospace field due to its low density and high melting point, and in the biomedical area owing to its high corrosion resistance and excellent biocompatibility when in contact with tissues or bones of the human body. The research novelty of this work is the aggregation of all kinds of data from the last 20 years of investigation about Ti–6Al–4V parts manufactured via SLM and EBM, namely information related to residual stresses (RS), as well as the influence played by different heat treatments in reducing porosity and increasing mechanical properties. Throughout the report, it can be seen that the expected microstructure of the Ti–6Al–4V alloy is different in both manufacturing processes, mainly due to the distinct cooling rates. However, heat treatments can modify the microstructure, reduce RS, and increase the ductility, fatigue life, and hardness of the components. Furthermore, distinct post-treatments can induce compressive RS on the part’s surface, consequently enhancing the fatigue life.