bluuebunny/crossref_metadata_2025 huggingface dataset:
MOOD BOX: AS A FORM OF RECOGNIZING THE MENTAL EMOTIONS OF KEDUNG PADARINGAN 2 ELEMENTARY SCHOOL STUDENTS AS THE FIRST STEP TOWARDS A HEALTHY AND COMFORTABLE SCHOOL
Authors: Zahro Ainin Zain , Fakhurrozi Alifian Akhsan , Safitri Anggun , Nurshoba Aurelia Marsha , Panggabean Destyn , Wicaksono Kresna , Salsabila Malinda , Evi Rizal Nasywa Nabiilah , Amla Nur Faridatul , Samudro Paksi , Fadila Rahayu Deka , Sinaga Yanti Hosianna Tri Agus , Fakhriyyah Zahrotul
Published: January 2024

Basic education is an important aspect in a country's development. The comfort of children's learning in elementary schools (SD) has a crucial role in determining the quality of their education. Children's emotions can affect their learning comfort, and managing children's moods from an early age is very necessary. One effort to increase children's learning comfort at Kedung Elementary School, Kepanjen, Malang Regency is through the implementation of the Mood Box Work Program (Proker). This program aims to create positive learning conditions, stimulate learning motivation, and reduce the impact of stress due to changes in learning patterns. In this journal, we will describe the Mood Box Program and the results of its evaluation of children's learning comfort at Kedung Elementary School. We will explore the methods, approaches and changes produced by this program in an effort to improve the quality of learning at this school. A deeper understanding of the impact of this program can provide a better view of the importance of a positive and comfortable learning environment for children in these challenging times. Through analysis and reflection on the Mood Box Program, we hope to make a positive contribution in efforts to improve the quality of basic education at Kedung Elementary School, as well as provide insight for policy makers, educators and researchers in understanding the importance of creating a learning environment that is conducive to the development of children in future.
HEALTH EDUCATION ABOUT CLEAN AND HEALTHY LIVING BEHAVIOR AT SDN 2 KEDUNG PEDARINGAN KEPANJEN, MALANG REGENCY
Authors: Tsurayya Nabila Hilmi
Published: January 2024

Perilaku Hidup Bersih dan Sehat (PHBS) is essential for everyday life and can be implemented invarious settings. Schools, as influential institutions, have a crucial role in shaping students' clean andhealthy living behavior. This study aimed to enhance students' knowledge of PHBS and their willingnessto practice it in school. We conducted a quantitative survey using a One Group Pre-test and Post-testdesign, involving all students from grades 1 to 5 at SD Negeri Kedung Pedaringan 2 in Malang Regency.Observations revealed that some students already practiced certain aspects of PHBS, such ashandwashing and proper waste disposal, albeit without recognizing their connection to a Clean andHealthy Lifestyle. To address this, we provided educational materials, using posters for grades 1-3 andPowerPoint presentations for grades 4 and 5. The engaging materials captured the students' interest, andthey actively participated, including singing about PHBS. Post-tests indicated a significant increase intheir knowledge and enthusiasm for implementing PHBS. In conclusion, PHBS is vital in everyday life,particularly within the school environment. This study emphasized the role of teachers in guidingstudents and fostering strong teacher-student relationships. By promoting PHBS, schools can becomecleaner, healthier, and disease-free environments. Future research should delve deeper into enhancingstudents' understanding and interest in their school's PHBS environment. The hope is that such effortswill continue to improve the cleanliness and health of school environments, ultimately benefitingstudents and the community as a whole.
NUTRIENT`S POCKET GAMES THE EFFECTIVENESS OF INTERACTIVE MEDIA IN UNDERSTANDING CHILD NUTRITION IN KEDUNGPEDARINGAN II KEPANJEN ELEMENTARY SCHOOL STUDENTS
Authors: Halizah Amilatun Nur , Soesanto Muhammad Ravi Maulana , Risky Aulia Dwi Nur
Published: January 2024

Child nutrition is important for children's physical and cognitive development. However,

many children do not have a good understanding of child nutrition. Interactive media has the potential

to be an effective tool to teach children about child nutrition. This study aims to determine the

effectiveness of interactive media (Game Saku Gizi) in improving students' understanding of child

nutrition. This study used a pre-experimental research design with a one-group pretest-posttest design.

The participants of this study were a group of grade 1 to grade 5 elementary school students at SDN

Kedungpedaringan II Kepanjen, Indonesia. The results showed that there was a significant difference

in students' understanding of child nutrition between the pretest and posttest. This means that the useof interactive media (Game Saku Gizi) can improve students' understanding of child nutrition. Thefindings of this study have important implications for the use of interactive media in education.Interactive media can be used to develop educational games and simulations that can help students to learn about various topics, including child nutrition. However, it is important to note that interactivemedia is not a magic bullet. It is important to use interactive media along with other teaching methods,such as lectures and discussions. In addition, it is also important to choose interactive media that isappropriate for the age and learning level of the students. Based on the results of the study, it can beconcluded that the Nutritional Pocket Game can improve the nutritional knowledge of students of
IMPROVING THE QUALITY OF LIFE OF THE ELDERLY THROUGH BODY MASS INDEX SCREENING IN MALANG
Authors: Masfi Achmad , Afiani Nurma
Published: January 2024

Introduction: The welfare of the elderly can be reflected through their health conditions, both physically, spiritually, and socially, which allows everyone to live a productive life socially and economically. Modern lifestyles have changed human attitudes and behaviors, including diet, smoking, alcohol consumption and drugs as a lifestyle so that patients with degenerative diseases (diseases due to decreased organ function) are increasing and threatening lives. Purpose: The purpose of this community service is to screen the Body Mass Index (BMI) the elderly. Methods: The methods used are measurement and examination as well as providing education. The methods used are measurement and examination as well as providing education. The method of measuring Body Mass Index and counseling about healthy lifestyles for the elderly in Malang. Results: The results of the screening conducted, of the 55 participants who took part in the examination, found 19 people were often active, 11 people experienced problems related to physical activity with infrequent activity, and 15 people experienced problems very rarely active. Conclusions: This community service runs smoothly and can provide benefits to the community. The results of this community service can be used as a reference for improving the health status of the elderly
QUARTER LIFE CRISIS: FACING REALITY THAT DOESN'T MATCH EXPECTATIONS AND PLANS
Authors: Putri Kurniawati Amalia , Ningrum Fiyona Yulita , Maulida Marshanda Rachma
Published: January 2024

Quarter life crisis occurs when a person reaches the age of 18 to 29 years. A person will begin to find it difficult to face the world, find it difficult to control their emotions, and begin to question whether the life they are living is the right life. So there is a need for more information about the quarter life crisis. One solution that can be considered is to provide information and disseminate it so that individuals recognize and understand how to respond to the quarter life crisis by using posters. The research method used in this research is qualitative with a survey of Instagram posts with the characteristics of respondents, namely people aged 20 years and over who have Instagram and see posts that researchers have posted on the Instagram page. The instrument used was in the form of an Instagram post containing the meaning, characteristics and factors causing the quarter life crisis which were then analyzed using descriptive analysis. The results of this research show that the majority of respondents are experiencing a quarter life crisis. It is hoped that with this post about the quarter life crisis, they will be able to realize that they are experiencing a quarter life crisis and can face the appropriate measures.
VIDEO CHAMPAIGN: DECISION MAKING IN QUARTER LIFE CRISIS PHASE
Authors: Ramadhani Anggie Selvia , Annajah Nabiilah , Putri Eka Cahyani Aulia , Ardiningrum Hapsari
Published: January 2024

The development of media technology is very rapid and with advances in technology that are increasingly developing day by day, the use of social media which can be accessed from mobile phones, the dominance of social media which is widely used, such as Instagram, is a favorite among Indonesian people, especially young people, with the number of users accessing it. Instagram is around 79% with various campaigns or interesting content. The campaign regarding the Quarter Life Crisis that has been created will be uploaded to Instagram with the aim of finding out how informative and interesting the campaign uploaded to Instagram is for teenage Instagram users. A quarter-life crisis is a crisis condition experienced in their 20s. Someone who is in Quarter Life Crisis often experiences emotional problems, where he is always confused about the various available options. The method used to create the campaign was data collection method through random interviews with FIK students and using the publication method via Instagram. The results obtained were seen through Instagram insights with a total audience of 497 users, 106 users liked, 35 users commented and 21 users shared.
Model Pembelajaran Inquiry Sebagai Solusi dalam Menghadapi Perkembangan Teknologi Informasi di Kalangan Siswa SMP Batam
Authors: Mangarimbun Gultom  , Talizaro Tafanao
Published: January 2024

Perkembangan teknologi informasi yang semakin maju di era globalisasi sangat berpengaruh terhadap dunia pendidikan karena mudahnya Informasi yang di dapat dalam internet menjadikan siswa malas untuk menggali dan menyelesaikan tanggung jawabnya menyelesaikan masalah-masalah tugas di sekolah. Penulis melihat perlu suatu desain metode pembelajaran untuk membantu guru dalam menyelesaikan masalah ini. Metode yang paling dekat dalam kasus ini dari hasil penjajakan literatur adalah metode Pembelajaran Inquiry. Tulisan ini diharapkan mampu untuk menjawab permasalahan yang sedang terjadi di dalam lingkungan siswa SMP Visi Kudus dalam menyerap, menggunakan dan memanfaatkan inflasi Informasi yang terjadi karena kemajuan teknologi. Penelitian ini merupakan penelitian deskriptif, karena penelitian ini bertujuan untuk mengetahui pengaruh dan pemanfaatan perkembangan teknologi informasi dan komunikasi berdasarkan fakta dan fenomena yang terjadi serta mempelajari hubungan dari sebab dan akibat dari pengaruh perkembangan teknologi Informasi dan komunikasi. Hasil dari penelitian ini adalah model pembelajaran inquiry di SMP Visi Kudus dapat meminimalisir penyalahgunaan inflasi informasi yang bercampur aduk di dalam media teknologi. Oleh sebab itu, penggunaan metode berbasis Inkuiri dapat meningkatkan kemampuan siswa untuk menyerap dan memilah Infalsi Informasi dari kemajuan teknologi dan siswa dapat menggunakan Informasi yang melimpah untuk memecahkan masalah.
Peran Guru PAK dalam Memanfaatkan Teknologi Untuk Meningkatkan Kualitas Pembelajaran
Authors: Frilia Kartini Sipahutar  , Zega Yunardi Kristian
Published: January 2024

Teknologi yang semakin canggih dari tahun ke tahun tentu membawa pengaruh besar bagi dunia, terutama dalam bidang pendidikan. Hal ini juga mempengaruhi bagaimana kualitas pembelajaran di sekolah dan dampaknya terhadap peserta didik. Tujuan dilakukan penelitian ini adalah untuk menjelaskan peran guru PAK dan manfaat teknologi dalam dunia pendidikan, serta cara-cara yang dapat dilakukan oleh seorang guru PAK dalam memanfaatkan teknologi yang ada untuk meningkatkan kualitas pembelajaran. Metode yang digunakan dalam penulisan ini adalah metode kualitatif dengan pendekatan studi kepustakaan. Hasil penelitian yang didapatkan adalah dengan pemanfaatan teknologi yang tepat ternyata dapat meningkatkan kualitas pembelajaran. Maka selain menjadi contoh yang nyata untuk bisa diteladani bagi peserta didik, guru PAK juga haruslah melek teknologi sehingga dapat memanfaatkan teknologi dalam meningkatkan kualitas pembelajaran dan menciptakan generasi penerus yang juga berkualitas, karena guru yang tidak melek teknologi akan mengalami kesulitan dalam memilih model pembelajaran yang akan diterapkan dan juga akan ketinggalan oleh perkembangan zaman yang semakin canggih dan modern.
Teacher And Student Book Analysis Integrated Thematic Character of Pancasila Student Profile in Grade I SD or MI Theme I is Myself
Authors: Suharsiwi  , Novia Sari
Published: January 2024

This study aims to determine: 1) to find out the literature review of thematic teacher books integrated character profiles of Pancasila students in grade I SD or MI Theme I Myself. 2) to find out the literature review of thematic students' books integrated with the character profile of Pancasila students in grade I SD or MI Theme I Myself. The research approach used is a qualitative library research approach or library research. The type of research used in this research is text study or textbook analysis. Analysis of textbooks in the form of integrated thematic teacher and student books for the 2013 curriculum for grade 1 SD or MI theme 1: myself. The results of the study show that: 1) In the thematic teacher's book integrated curriculum 2013 grade 1 SD or MI Theme 1: Myself, there are 14 character values. The 14 character values are discipline, friendly or communicative, religious, honest, hard work, independent, responsible, tolerance, curiosity, love to read, creative, national spirit, love for the homeland, and caring (social care). In the values of the Pancasila student profile there are all or there are 6 indicators or values of the Pancasila student profile. The 6 values of the Pancasila student profile are faith and piety to God Almighty and noble character, mutual cooperation, independence, global diversity, critical reasoning, and creativity. 2) In the thematic integrated student book 2013 curriculum grade 1 SD or MI Theme 1: Myself, there are 10 character values. The 10 character values are religious, friendly or communicative, hard work, independent, responsible, disciplined, love to read, creative, love the homeland, and tolerance. In the values of the Pancasila student profile, there are all or 6 indicators or values of the Pancasila student profile. The 6 values of the Pancasila student profile are faith and piety to God Almighty and noble character, mutual cooperation, independence, global diversity, critical reasoning, and creativity.
Strategi Keluarga Kristen dalam Membangun Anak Yang Berprestasi di Sekolah
Authors: Malau Indah Margaretha , Desika Sihombing  , Ganda Marito  , Ronald Sianipar
Published: January 2024

Keluarga merupakan unit terpenting dalam struktur kemasyarakatan. Keluarga memegang peranan pembinaan pendidikan anak-anak dalam keluarga. Banyak para ahli yang memberikan predikat bahwa keluarga merupakan tempat yang pertama dan utama dalam memperoleh pendidikan. Orang tua memiliki peranan penting dalam meningkatkan perkembangan dan prestasi belajar anak. Tanpa dorongan dan rangsangan orang tua, maka perkembangan prestasi belajar anak akan mengalami hambatan. Oleh sebab itu, tujuan dari penelitian ini adalah untuk memberikan gambaran kepada keluarga Kristen untuk menolong  bagaimana strategi dalam memmbangun anak yang berprestasi, khususnya di sekolah. Penelitian ini menggunakan metode penelitian kualitatif deskriptif dengan pendekatan pustaka. Hasil yang diperoleh, antara lain: Pertama, orang tua harus dapat menjadi seorang pembimbing bagi anak. Kedua, orang tua perlu memberikan fasilitas belajar yang memadai di dalam keluarga. Ketiga, orang tua perlu memberikan motivasi belajar kepada setiap anak-anaknya. Keempat, orang tua harus terus menerus pendukung utama anak dalam mencapai prestasi. Kelima, orang tua harus dalam mengetahui kelebihan dan kekurangan anak-anaknya dan membantu mereka dalam mengatasi setiap kekurangannya dan mendukung kelebihannya agar dapat lebih ditingkatkan. Keenam, Orang tua harus dapat menjadi mentor yang baik bagi setiap anak-anaknya.
Strategi Guru Pendidikan Agama Kristen dalam Memotivasi Siswa Membaca Alkitab di Sekolah Dasar
Authors: Meliana Agustina Simanjuntak  , Ronald Sianipar
Published: January 2024

Membaca Alkitab merupakan hal yang sangat penting bagi peserta didik Kristen, khususnya di tingkat sekolah dasar. Membaca Alkitab dapat membantu peserta didik memperoleh informasi tentang Tuhan Yesus dari Firman Tuhan yang telah dibaca. Namun, faktanya yang terjadi saat ini, masih banyak peserta didik yang mengabaikan untuk membaca Alkitab dan lebih mengutamakan untuk bermain dengan Gadgetnya. Oleh sebab itu, tujuan dari penelitian ini adalah untuk membantu peserta didik supaya tidak kesulitan dalam membaca Alkitab dan guru PAK harus membantu peserta didik untuk mengatasi masalah kesulitan membaca Alkitab. Metode dalam penelitian ini adalah metode kualitatif deskriptif dengan pendekatan studi pustaka. Hasil dari penelitian ini adalah guru PAK memiliki peran yang penting dalam mendidik peserta didik, khususnya peserta didik di tingkat sekolah dasar. Jadi, guru Pendidikan Agama Kristen harus memiliki berbagai strategi mengajar yang tepat sehingga dapat memunculkan motivasi peserta didik untuk senang dalam membaca Alkitab.
Kreativitas Guru Sekolah Minggu, Penggunaan Media Daring dalam Peningkatan Minat Beribadah Anak Usia 6-8 Tahun pada Masa Pandemi Covid-9 di Gereja Bethel Indonesia World Trade Center Serpong
Authors: Christina Mendrofa  , Gideon Sutrisno  , Yusak Tanasyah
Published: January 2024

Pentingnya Anak-anak sebagai generasi penerus dalam pandangan Tuhan, bangsa, dan gereja sering terabaikan, terutama dalam pelayanan anak di sekolah miggu selama pandemi Covid-19. Tantangan yang dihadapi melibatkan kurangnya persiapan, pembagian kelas tanpa mempertimbangkan usia anak, dan dukungan finansial minim dari orang tua, yang menghambat kreativitas dan perkembangan sekolah minggu. Sementara itu kehadiran anak-anak mengalami penurunan dengan drastis yang disertai dengan persepsi yang rendah terhadap kualitas sekolah minggu. Penelitian ini menggunakan metode kualitatif melalaui teknik observasi, wawancara dan dokumentasi. Sehingga ditemukan bahwa GBI WTC Serpong memberikan warna yang berbeda dengan kreativitas guru sekolah minggu yang berhasil meningkatkan minat anak-anak melalui kostum, dekorasi kelas, dan acara ibadah kreatif. Dukungan penuh dari gereja, semangat guru yang terus berkembang, dan penggunaan media, terutama Aplikasi Zoom, berhasil mempertahankan minat anak-anak selama pandemi. Meskipun ada kendala, kesuksesan Sekolah Minggu GBI WTC Serpong, terutama dalam meningkatkan partisipasi anak-anak dengan menjadikannya contoh bagi gereja lain.
Analisis Kritis Pandangan Nomianisme dan Asketisme Serta Integrasinya dalam Pendidikan Agama Kristen
Authors: Girsang Chenlin Doura Vivian , David Folind Zega  , Amanda Kilala  , Candra Gunawan Marisi
Published: January 2024

Perkembangan teologi mulai dari abad pertama sampai detik ini telah menghasilkan banyak sekali ajaran-ajaran atau paham-paham teolog yang baru dalam kekristenan. Dan tidak jarang ajaran-ajaran tersebut merupakan ajaran-ajaran yang sesat atau bisa juga disebut bidat-bidat. Hal ini bisa saja terjadi karena salah menafsir atau mencoba menggabungkan Alkitab dengan kebiasaan saat itu. Salah satu dari ajaran sesat atau bidat yang muncul dalam abad pertama adalah nomianisme dan asketisme. Penelitian ini bertujuan untuk menjelaskan pengertian yang benar mengenai nomianisme dan asketisme serta integrasinya dalam dunia pendidikan agama Kristen sehingga kelak diharapkan generasi ke depan bahkan orang-orang Kristen di zaman akhir ini tidak terikut dengan paham-paham yang salah ini. Dan memiliki pegangan yang kuat dalam kehidupannya sebagai orang Kristen. Metode penelitian yang digunakan adalah penelitian deskriptif dengan pendekatan studi pustaka. Dan setelah dilakukan pengumpulan data, kemudian di analisa dan diberikan argumentasi, dimana argumentasi tersebut akan ditarik suatu kesimpulan dalam penelitian ini. Nomianisme merupakan sebuah ajaran yang berpatokan dengan hukum. Dalam hal ini hukum Taurat. Sedangkan asketisme adalah sebuah ajaran yang dimana mengajarkan menyangkal diri supaya bisa mencapai kepada kesempurnaan dan sang Ilahi. Hasil yang terlihat pada ajaran-ajaran ini jika diintegrasikan ke dalam pendidikan agama Kristen atau PAK sebagai salah satu pengajaran untuk semakin menunjukkan hidup seseorang yang ada di dalam Kristus, yang menyangkal diri memikul salib, mentaati perintah-perintah Tuhan, maka ajaran-ajaran ini dapat dipakai. Namun tidak dapat digunakan sebagai ajaran untuk memperoleh keselamatan.
Rohingya Crisis from a Geopolitical Lens: Power Struggle over Regional Hegemony shadowing the Humanitarian needs
Authors: Sakib A. B. M. Najmus , Rashid Zarin Tasnim
Published: January 2024

Abstract: Rakhine state in Myanmar is strategically located at the center of a power struggle between emerging Asian nations. Consequently, the strategic position has become the crucial factor that fans the fire behind the current Rohingya issue. As a minority group in Myanmar, the Rohingya have struggled for citizenship for decades. To escape the state-orchestrated persecution, a large number of Rohingya took shelter in neighboring Bangladesh. Under the cloak of geopolitical issues, the Rohingya Crisis has lingered for five years with no clear path to resolution. While India attempts to pursue its “Act East Policy,” China seeks to incorporate Myanmar into its “One Belt, One Road” initiative. A further aggravating factor is a threat posed by US military forces in Southeast Asia to China’s interests. However, as a neighboring nation of Myanmar, Bangladesh became entangled in the conflict between the Regional Competitors. The study argues that the geopolitical interest of bordering countries and the economic interest of the military regime have exacerbated the situation and are one of the primary reasons why the crisis has not been resolved. The purpose of this study is to analyze how and why the geopolitical consequences of the Rohingya crisis outrun the humanitarian demands of this crisis. Through content analysis of previous scholarly publications, the conclusions of the research have centered on how the fight for regional hegemony makes the Rohingya crisis more vulnerable.
Effects of Emotional Intelligence and Smartphone Dependence on Mental Health among College Students in Henan Province, China
Authors: Sun Yuanyuan , Zhao Wenbo
Published: January 2024

Besides ushering in an unprecedented level of material convenience for humanity, mobile phones have inevitably led to numerous mental health issues. The phenomenon of psychological problems arising from dependence, addiction, and problematic usage of the internet and mobile phones has become increasingly widespread. Various social adversities stemming from mental health issues have garnered comprehensive attention across society. At a certain university in Henan Province, the degree of mobile phone dependence among college students is at a moderate level. Therefore, studying the students at this university is representative of higher education institutions throughout Henan Province and holds considerable research value. This study surveyed 335 individuals from a university in Henan Province and conducted empirical analysis. The findings indicate that mobile phone dependency negatively impacts mental health. The scale measuring mobile phone dependency demonstrates a significant negative correlation with emotional intelligence. Moreover, emotional intelligence positively influences mental health.
Study on the Impact Mechanism of Transformational Leadership on Employee Innovative Behavior
Authors: Zhao Xiaoyan
Published: January 2024

This article is based on the context of China, aiming to clarify the relationship between transformational leadership and employee innovation behavior by exploring the relationship between transformational leadership and organizational commitment, and investigating the mediating role of organizational commitment in the relationship between transformational leadership and employee innovation behavior. Finally, this article analyzes the regulatory relationship between transformational leadership and employee innovation behavior through organizational climate. By delving into these issues, the aim of this article is to provide effective theoretical guidance for the study of transformational leadership on employee innovation behavior and to provide feasible recommendations for business management to promote the development of employee innovation behavior. his article uses a questionnaire survey method to investigate 397 employees of Company S in China. The results of the multiple linear regression analysis show that transformational leadership positively enhances employee innovation behavior, transformational leadership positively enhances organizational commitment, organizational commitment positively enhances employee innovation behavior, transformational leadership indirectly enhances employee innovation behavior by improving organizational commitment, and organizational climate promotes the positive relationship between transformational leadership and employee innovation behavior. This article presents recommendations from the organizational level, leadership level, and employee level, respectively.
Intrinsic protein disorder is insufficient to drive subnuclear clustering in embryonic transcription factors
Authors: Hannon Colleen E , Eisen Michael B
Published: January 2024

Modern microscopy has revealed that core nuclear functions, including transcription, replication, and heterochromatin formation, occur in spatially restricted clusters. Previous work from our lab has shown that subnuclear high-concentration clusters of transcription factors may play a role in regulating RNA synthesis in the early Drosophila embryo. A nearly ubiquitous feature of eukaryotic transcription factors is that they contain intrinsically disordered regions (IDRs) that often arise from low complexity amino acid sequences within the protein. It has been proposed that IDRs within transcription factors drive co-localization of transcriptional machinery and target genes into high-concentration clusters within nuclei. Here, we test that hypothesis directly, by conducting a broad survey of the subnuclear localization of IDRs derived from transcription factors. Using a novel algorithm to identify IDRs in the Drosophila proteome, we generated a library of IDRs from transcription factors expressed in the early Drosophila embryo. We used this library to perform a high-throughput imaging screen in Drosophila Schneider-2 (S2) cells. We found that while subnuclear clustering does not occur when the majority of IDRs are expressed alone, it is frequently seen in full-length transcription factors. These results are consistent in live Drosophila embryos, suggesting that IDRs are insufficient to drive the subnuclear clustering behavior of transcription factors. Furthermore, the clustering of transcription factors in living embryos was unaffected by the deletion of IDR sequences. Our results demonstrate that IDRs are unlikely to be the primary molecular drivers of the clustering observed during transcription, suggesting a more complex and nuanced role for these disordered protein sequences.
DNA methylation-environment interactions in the human genome
Authors: Johnston Rachel A. , Aracena Katherine A. , Barreiro Luis B. , Lea Amanda J. , Tung Jenny
Published: January 2024

Previously we showed that a massively parallel reporter assay, mSTARR-seq, could be used to simultaneously test for both enhancer-like activity and DNA methylation-dependent enhancer activity for millions of loci in a single experiment (Lea
et al
., 2018). Here we apply mSTARR-seq to query nearly the entire human genome, including almost all CpG sites profiled either on the commonly used Illumina Infinium MethylationEPIC array or via reduced representation bisulfite sequencing. We show that fragments containing these sites are enriched for regulatory capacity, and that methylation-dependent regulatory activity is in turn sensitive to the cellular environment. In particular, regulatory responses to interferon alpha (IFNA) stimulation are strongly attenuated by methyl marks, indicating widespread DNA methylation-environment interactions. In agreement, methylation-dependent responses to IFNA identified via mSTARR-seq predict methylation-dependent transcriptional responses to challenge with influenza virus in human macrophages. Our observations support the idea that pre-existing DNA methylation patterns can influence the response to subsequent environmental exposures—one of the tenets of biological embedding. However, we also find that, on average, sites previously associated with early life adversity are not more likely to functionally influence gene regulation than expected by chance.
An engineered biosensor enables dynamic aspartate measurements in living cells
Authors: Davidsen Kristian , Marvin Jonathan S , Aggarwal Abhi , Brown Timothy A , Sullivan Lucas B
Published: January 2024

Intracellular levels of the amino acid aspartate are responsive to changes in metabolism in mammalian cells and can correspondingly alter cell function, highlighting the need for robust tools to measure aspartate abundance. However, comprehensive understanding of aspartate metabolism has been limited by the throughput, cost, and static nature of the mass spectrometry based measurements that are typically employed to measure aspartate levels. To address these issues, we have developed a GFP-based sensor of aspartate (jAspSnFR3), where the fluorescence intensity corresponds to aspartate concentration. As a purified protein, the sensor has a 20-fold increase in fluorescence upon aspartate saturation, with dose dependent fluorescence changes covering a physiologically relevant aspartate concentration range and no significant off target binding. Expressed in mammalian cell lines, sensor intensity correlated with aspartate levels measured by mass spectrometry and could resolve temporal changes in intracellular aspartate from genetic, pharmacological, and nutritional manipulations. These data demonstrate the utility of jAspSnFR3 and highlight the opportunities it provides for temporally resolved and high throughput applications of variables that affect aspartate levels.
Variation in the basal immune state and implications for disease
Authors: Souquette Aisha , Thomas Paul G
Published: January 2024

Analysis of pre-existing immunity and its effects on acute infection often focus on memory responses associated with a prior infectious exposure. However, memory responses occur in the context of the overall immune state and leukocytes must interact with their microenvironment and other immune cells. Thus, it is important to also consider non-antigen-specific factors which shape the composite basal state and functional capacity of the immune system, termed here as I0 (‘I naught’). In this review, we discuss the determinants of I0. Utilizing influenza virus as a model, we then consider the effect of I0 on susceptibility to infection and disease severity. Lastly, we outline a mathematical framework and demonstrate how researchers can build and tailor models to specific needs. Understanding how diverse factors uniquely and collectively impact immune competence will provide valuable insights into mechanisms of immune variation, aid in screening for high-risk populations, and promote the development of broadly applicable prophylactic and therapeutic treatments.
Hippocampome.org v2.0: a knowledge base enabling data-driven spiking neural network simulations of rodent hippocampal circuits
Authors: Wheeler Diek W. , Kopsick Jeffrey D. , Sutton Nate , Tecuatl Carolina , Komendantov Alexander O. , Nadella Kasturi , Ascoli Giorgio A.
Published: January 2024

Hippocampome.org is a mature open-access knowledge base of the rodent hippocampal formation focusing on neuron types and their properties. Hippocampome.org v1.0 established a foundational classification system identifying 122 hippocampal neuron types based on their axonal and dendritic morphologies, main neurotransmitter, membrane biophysics, and molecular expression. Releases v1.1 through v1.12 furthered the aggregation of literature-mined data, including among others neuron counts, spiking patterns, synaptic physiology,
in vivo
firing phases, and connection probabilities. Those additional properties increased the online information content of this public resource over 100-fold, enabling numerous independent discoveries by the scientific community. Hippocampome.org v2.0, introduced here, besides incorporating over 50 new neuron types, now recenters its focus on extending the functionality to build real-scale, biologically detailed, data-driven computational simulations. In all cases, the freely downloadable model parameters are directly linked to the specific peer-reviewed empirical evidence from which they were derived. Possible research applications include quantitative, multiscale analyses of circuit connectivity and spiking neural network simulations of activity dynamics. These advances can help generate precise, experimentally testable hypotheses and shed light on the neural mechanisms underlying associative memory and spatial navigation.
Collecting whales: processes and biases in Nordic museum collections
Authors: Delsett Lene Liebe
Published: January 2024

Whales are unique museum objects that have entered collections in many ways and for different reasons. This work studies three Nordic natural history museum collections in Norway and Denmark with more than 2,500 whale specimens in total, and gathers the available biological and collection data on the specimens, which include skeletal elements, foetuses and organs preserved in ethanol or formalin, and a few dry-preserved organs. It finds that influx of specimens, which were mainly locally common species that were hunted, to the collections, mainly happened in the latest 1800s and earliest 1900s, fuelled by research trends, nation building, local whaling, and colonial mechanisms. Norway was a major whaling nation, but the largest hunt for whales in the Southern Ocean in the mid-1900s is not reflected in the Norwegian museum collections, probably because of the commercial focus of the whaling industry and logistical challenges, combined with limited research interest in zoological specimens at that time. The results demonstrate that it is important to understand these processes and the resulting biases for future research, outreach, and conservation.
Effect of dynamic taping on neck pain, disability, and quality of life in patients with chronic non-specific neck pain: a randomized sham-control trial
Authors: Sidiq Mohammad , Chahal Aksh , Janakiraman Balamurugan , Kashoo Faizan , Kumar Kedia Sharad , Kashyap Neha , Hirendra Rai Richa , Vyas Neha , Veeragoudhaman T.S. , Vajrala Krishna Reddy , Yadav Megha , Zafar Shahiduz , Jena Sanghamitra , Sharma Monika , Baranwal Shashank , Alghadier Mshari , Alhusayni Abdullah , Alzahrani Abdullah , Selvan Natarajan Vijay
Published: January 2024

Background

In 2020, 203 million people experienced neck pain, with a higher prevalence in women. By 2050, it is predicted that neck pain will affect 269 million people, representing a 32.5% increase. Physical rehabilitation is often employed for the treatment of chronic non-specific neck pain (CNSNP) and the associated functional loss. Taping is frequently used as an adjunct treatment alongside primary physical rehabilitation. Unlike kinesio tape (KT), the therapeutic benefits of dynamic tape (DT) have not been thoroughly explored and documented in non-athletic conditions. Therefore, the aim of this trial was to determine the effects of DT on pain, disability, and overall well-being in individuals experiencing CNSNP.

Methods

A prospective parallel-group active controlled trial was conducted at a single center, involving 136 patients with CNSNP, randomly allocated in a 1:1 ratio. The sham taping group (STC) received standard physiotherapy care (n = 67) alongside DT without tension, while the dynamic taping group (DTC) (n = 69) underwent standard cervical offloading technique with appropriate tension in addition to standard physiotherapy care. Demographic information and three patient-reported outcome measures (PROMs), namely the Neck Disability Index (NDI), Visual Analogue Scale (VAS), and the World Health Organization—Five Well-Being Index (WHO-5), were collected for each participant at three time points (baseline, four weeks post-taping, and four weeks follow-up).

Results

At baseline, no significant differences were observed between the STC and DTC for any outcome measure. Notably, all three PROMs exhibited a significant improvement from baseline to four weeks post-intervention, with moderate to small effect sizes (NDI ηp2 = 0.21, VAS ηp2 = 0.23, and WHO-55 ηp2 = 0.05). The WHO-5 scores for both groups demonstrated improvement from baseline through follow-up (p < 0.001). The NDI and VAS scores ameliorated from baseline to the four weeks post-taping period, with marginal improvements observed during the four weeks follow-up.

Conclusion

The incorporation of DT as an adjunct to standard physiotherapy care yielded enhancements in pain levels, functional disability, and well-being among patients with CNSNP when compared to the sham group. However, the sustainability of these improvements beyond the taping period lacks statistical significance and warrants further validation.
Prevalence of intestinal parasites and comparison of detection techniques for soil-transmitted helminths among newly arrived expatriate labors in Jeddah, Saudi Arabia
Authors: Al-Refai Mohammad F. , Wakid Majed H.
Published: January 2024

Background

Diversity in clinical signs and symptoms are associated with soil transmitted diseases (STD), which are spread to humans by intestinal worms and transmitted in a variety of ways. There is a need for the present study, which aimed to investigate the prevalence of intestinal parasites and to compare between the common detection techniques for soil-transmitted helminths (STHs) among newly arrived expatriate labors in Jeddah, Saudi Arabia.

Methods

A total of 188 stool samples were analyzed by macroscopic examination, and microscopic examination using direct iodine smear and the formal ether sedimentation technique. Trichrome and modified Kinyoun’s stains were used to confirm the morphology of any detected protozoa stages and oocyst of Cryptosporidium, respectively. A chromatographic immunoassay kit was used for Entamoeba histolytica, Giardia lamblia and Cryptosporidium. In addition, real-time PCR was employed only to identify various STHs.

Results

Out of 188, several types of parasites were detected in 35 samples (18.62%), of which some with multiple infections. Nine samples (4.79%) were positive for Entamoeba coli, seven samples (3.72%) for Trichuris trichiura, six samples (3.19%) for Necator americanus, four samples (2.13%) for Strongyloides stercoralis, four samples (2.13%) for Ascaris lumbricoides, four samples (2.13%) for E. histolytica, three samples (1.60%) for Blastocystis hominis and two samples (1.06%) for Ancylostoma duodenale. In comparison between laboratory techniques for STHs, real-time PCR was able to detect the DNA of 19 samples (10.1%) followed by Ritchie sedimentation technique (18, 9.6%), and direct smear (7, 3.7%) (p > 0.05).

Conclusion

The high rate of newly arrived foreign workers infected with intestinal parasites could lead to a risk to society. Continuous and regular surveys are needed to deal with the occurrence of intestinal parasitic infections including STHs. To improve the identification of these infections, we recommend a supporting infrastructure for the application of concentration methods and molecular assays.
Brechas académicas en matemática escolar: entre el sexismo y el sesgo
Authors:   , Pantoja De Prada Beltran Pedro
Published: January 2024

El informe examina un fenómeno paradójico en los desempeños escolares de matemática, donde se observan brechas a favor de las mujeres en los promedios de enseñanza media, pero a favor de los hombres en los resultados Simce. El objetivo del estudio es explorar la relación entre las creencias sexistas de profesores de matemática en enseñanza media y estas brechas. El informe consta de cinco partes. En la primera parte, se delimita el problema, se presentan los antecedentes, los objetivos y la perspectiva conceptual. La segunda parte describe las etapas de la investigación, el instrumento utilizado, su aplicación y el análisis de los resultados. En la tercera parte, se presentan los resultados que indican una mayor presencia de sexismo en los profesores en comparación con las profesoras. Sin embargo, no se encontró suficiente información para describir cómo esto se relaciona con las brechas de desempeño académico dentro de sus cursos. La cuarta parte del informe ofrece recomendaciones basadas en los resultados obtenidos. Por último, se reflexiona sobre el trabajo realizado en la asignatura de Trabajo Final 1, Trabajo Final 2 y se destacan los aportes del trabajo colaborativo durante este período, así como su aplicación práctica en la profesión.
Biorremediabilidad y biotoxicidad de suelos intemperizados y altamente contaminados con petróleo crudo
Authors:   , Rodríguez Córdova Leonardo Andrés
Published: January 2024

La extracción intensiva de petróleo fue una bullante actividad en la Región de Magallanes durante la primera mitad del siglo XX. Desde la primera evidencia de hidrocarburos en 1899, el oro negro impulsó a muchos aventureros a instalarse en el sector e iniciar actividades extractivas artesanales. Esto ocurrió hasta que en la década de 1940 el Gobierno de Chile, a través de la Corporación de Fomento de la Producción (CORFO) y de la Empresa Nacional del Petróleo (ENAP), comenzó la explotación más industrializada del recurso. Sin embargo, la precaria tecnología de la época produjo diversos sitios contaminados en forma de fosas de contención de petróleo que por la falta de normativa ambiental y/o el desconocimiento de las consecuencias de este pasivo ambiental, fueron abandonados sin medidas de mitigación. No fue hasta la década del 2000 que normativamente fue desarrollándose el marco regulatorio específico que obliga el tratamiento de este tipo de problemas ambientales. En este trabajo de tesis doctoral se realizó un estudio sobre la aplicación de un sistema de biorremediación para suelos altamente contaminados con petróleo crudo e intemperizados por más de 7 décadas en la Región de Magallanes en Chile. Para ello, se aplicó biorremediación ex-situ on-site aeróbica, utilizando turba de 2 tipos (autóctona y comercial) como agente abultante. Los resultados mostraron que la biorremediación es factible para este tipo de suelos, aunque el proceso no permite llegar a concentraciones contaminantes residuales dentro de los niveles normativos referenciales establecidos. Entre otros resultados obtenidos, se demostró que la turba Magallánica contiene microorganismos potencialmente degradadores del contaminante estudiado, por lo que además de agente abultante puede ser considerada un inóculo adecuado para el proceso degradativo del petróleo crudo envejecido e intemperizado de los suelos estudiados. Adicionalmente se mostró en este trabajo que el suelo contaminado y extensamente intemperizado en estudio aun presenta características biotóxicas medidas a partir de test de germinación de semillas, a pesar del extenso envejecimiento al que ha sido sometido durante 70 años. En este sentido es claro enfatizar que este tipo de suelos debe ser tratado dada la elevada biotoxicidad que aun presenta.
tesis de maestría Sustainable synthesis and extraction of pyrrolidones produced via reductive amination of levulinic acid
Authors:   , Meruane Anich Joaquín Andrés
Published: January 2024

Se ha propuesto que las 5-metil-N-sustituidas-2-pirrolidonas (5MPs) sean una alternativa a la NMP, un solvente común con varias aplicaciones industriales, comerciales y de consumo, debido a que el último se considera peligroso e indeseado por los reguladores. Las 5MPs se pueden producir mediante la aminación reductiva del ácido levulínico bioderivado, bajo la presencia de un catalizador y un agente reductor como el hidrógeno molecular. Sin embargo, la posterior separación de este producto, del medio de reacción, sigue siendo un desafío presente para la posterior viabilidad industrial y comercial. Este trabajo es el primer estudio sistemático para la separación de pirrolidonas a través del equilibrio líquido-líquido. La extracción con solventes es un tema fundamental en la química verde, y por lo tanto, se debe considerar no solo el rendimiento de separación, sino también los criterios ambientales, de salud y seguridad (EHS), así como los aspectos de viabilidad industrial y comercial. Por lo tanto, se utilizó COSMO-RS junto conjunto a variadas guías EHS para seleccionar los mejores solventes para la extracción de 5-metil-Nfenil-2-pirrolidona (PP) tanto de metanol como de agua. Los solventes más prometedores se estudiaron posteriormente en experimentos de equilibrio íquido-líquido ternarios con ambos productos y reactivos. Posteriormente, se probaron los solventes para la extracción de pirrolidona de una mezcla de reacción real, producida mediante una aminación reductiva catalizada por Pt_TiO2 en condiciones ambientales. Las predicciones y las validaciones posteriores de los coeficientes de distribución y selectividades muestran que el anisol y el limoneno son solventes prometedores para la extracción de PP tanto de medios acuosos como de MeOH. Ambos solventes también son alternativas atractivas debido a que son bio-basados, siendo el primero también recomendado por las guías de selección de solventes, mientras que el último tiene ciertas preocupaciones en algunos criterios ambientales.
A CRITICAL REVIEW OF THE APPLIED ASPECT OF SHADVIDHUPAKRAMA
Authors: Waghmare Madhuri , Hingane Anjali , Dhurde Smita
Published: January 2024

Different kinds of chikitsa have been recommended for disease therapy by the timeless science of life known as Ayurveda. Shadvidha upakrama is a very important chikitsa among those given. Upakrama expertise is critical in this situation. According to the Ayurvedic system, only two upakramas, bruhana (vruddhikara) and langhana (hrasakara), are primarily discussed. Specifically, santarpana and apatarpana. Satarpana is defined as an excessive intake of overly nutrient-dense food and a lack of physical activity. Apatarpaan is defined as consuming fewer, less nutrient-dense meals while engaging in more physical activity. Ayurveda has the potential to transform the current healthcare industry and create a more sustainable healthcare system in the future. Its prevention is better than cure philosophy, is to offer guidance for a healthy lifestyle that will promote general wellbeing. In Ayurveda, mandagni results in aamsanchiti, which causes santarpanjanya vyadhis like diabetes, kustha, jwar, libido, sthoulya, etc., and undernutrition results in apatarpan, which causes vyadhis like decreased physical strength, oja, varna, shukra, and reduced muscle power. Shadvidh upakrama is used to prevent and treat various diseases.