bluuebunny/crossref_metadata_2025 huggingface dataset:
Association between Circulating Fibroblast Growth Factor 21 and Aggressiveness in Thyroid Cancer
Authors: Kang Yea Eun , Kim Jung Tae , Lim Mi Ae , Oh Chan , Liu Lihua , Jung Seung-Nam , Won Ho-Ryun , Lee Kyungmin , Chang Jae Won , Yi Hyon-Seung , Kim Hyun Jin , Ku Bon Jeong , Shong Minho , Koo Bon Seok
Published: August 2019

Fibroblast growth factor 21 (FGF21) plays important roles in regulating glucose, lipid, and energy metabolism; however, its effects in tumors remain poorly understood. To understand the role of FGF21 in regulating tumor aggressiveness in thyroid cancer, serum levels of FGF21 were measured in healthy subjects and patients with papillary thyroid cancer (PTC), and expression levels of FGF21, FGF receptors (FGFRs), and β-klotho (KLB) were investigated in human thyroid tissues. The cell viability, migrating cells, and invading cells were measured in PTC cells after treatment with recombinant FGF21. Higher serum levels of FGF21 were found in patients with thyroid cancer than in control participants, and were significantly associated with body mass index (BMI), fasting glucose levels, triglyceride levels, tumor stage, lymphovascular invasion, and recurrence. Serum FGF21 levels were positively correlated with the BMI in patients with PTC, and significantly associated with recurrence. Recombinant FGF21 led to tumor aggressiveness via activation of the FGFR signaling axis and epithelial-to-mesenchymal transition (EMT) signaling in PTC cells, and AZD4547, an FGFR tyrosine kinase inhibitor, attenuated the effects of FGF21. Hence, FGF21 may be a new biomarker for predicting tumor progression, and targeting FGFR may be a novel therapy for the treatment of obese patients with PTC.
Epigenetic Changes as a Target in Aging Haematopoietic Stem Cells and Age-Related Malignancies
Authors: Buisman Sonja C. , de Haan Gerald
Published: August 2019

Aging is associated with multiple molecular and functional changes in haematopoietic cells. Most notably, the self-renewal and differentiation potential of hematopoietic stem cells (HSCs) are compromised, resulting in myeloid skewing, reduced output of red blood cells and decreased generation of immune cells. These changes result in anaemia, increased susceptibility for infections and higher prevalence of haematopoietic malignancies. In HSCs, age-associated global epigenetic changes have been identified. These epigenetic alterations in aged HSCs can occur randomly (epigenetic drift) or are the result of somatic mutations in genes encoding for epigenetic proteins. Mutations in loci that encode epigenetic modifiers occur frequently in patients with haematological malignancies, but also in healthy elderly individuals at risk to develop these. It may be possible to pharmacologically intervene in the aberrant epigenetic program of derailed HSCs to enforce normal haematopoiesis or treat age-related haematopoietic diseases. Over the past decade our molecular understanding of epigenetic regulation has rapidly increased and drugs targeting epigenetic modifications are increasingly part of treatment protocols. The reversibility of epigenetic modifications renders these targets for novel therapeutics. In this review we provide an overview of epigenetic changes that occur in aging HSCs and age-related malignancies and discuss related epigenetic drugs.
The Abnormal CD4+T Lymphocyte Subset Distribution and Vbeta Repertoire in New-onset Rheumatoid Arthritis Can Be Modulated by Methotrexate Treament
Authors: Monserrat Jorge , Bohórquez Cristina , Gómez Lahoz Ana María , Movasat Atusa , Pérez Ana , Ruíz Lucía , Díaz David , Chara Luis , Sánchez Ana Isabel , Albarrán Fernando , Sanz Ignacio , Álvarez-Mon Melchor
Published: August 2019

Patients with long-term, treated, rheumatoid arthritis (RA) show abnormalities in their circulating CD4+ T-lymphocytes, but whether this occurs in recently diagnosed naïve patients to disease-modifying drugs (DMARDs) is under discussion. These patients show heterogeneous clinical response to methotrexate (MTX) treatment. We have examined the count of circulating CD4+ T-lymphocytes, and their naïve (TN), central memory (TCM), effector memory (TEM) and effector (TE) subsets, CD28 expression and Vβ TCR repertoire distribution by polychromatic flow cytometry in a population of 68 DMARD-naïve recently diagnosed RA patients, before and after 3 and 6 months of MTX treatment. At pre-treatment baseline, patients showed an expansion of the counts of CD4+ TN, TEM, TE and TCM lymphocyte subsets, and of total CD4+CD28− cells and of the TE subset with a different pattern of numbers in MTX responder and non-responders. The expansion of CD4+TEM lymphocytes showed a predictive value of MTX non-response. MTX treatment was associated to different modifications in the counts of the CD4+ subsets and of the Vβ TCR repertoire family distribution and in the level of CD28 expression in responders and non-responders. In conclusion, the disturbance of CD4+ lymphocytes is already found in DMARD-naïve RA patients with different patterns of alterations in MTX responders and non-responders.
Oligosaccharides Modulate Rotavirus-Associated Dysbiosis and TLR Gene Expression in Neonatal Rats
Authors: Azagra-Boronat Ignasi , Massot-Cladera Malén , Knipping Karen , van‘t Land Belinda , Tims Sebastian , Stahl Bernd , Knol Jan , Garssen Johan , Franch Àngels , Castell Margarida , Pérez-Cano Francisco J. , Rodríguez-Lagunas Maria J.
Published: August 2019

Colonization of the gut in early life can be altered through multiple environmental factors. The present study aimed to investigate the effects of 2’-fucosyllactose (2’-FL), a mixture of short-chain galactooligosaccharides/long-chain fructooligosaccharides (scGOS/lcFOS) 9:1 and their combination (scGOS/lcFOS/2’-FL) on dysbiosis induced during rotavirus (RV) diarrhea in neonatal rats, elucidating crosstalk between bacteria and the immune system. The dietary interventions were administered daily by oral gavage at days 2–8 of life in neonatal Lewis rats. On day 5, RV SA11 was intragastrically delivered to induce infection and diarrhea assessment, microbiota composition, and gene expression of Toll-like receptors (TLRs) in the small intestine were studied. All dietary interventions showed reduction in clinical variables of RV-induced diarrhea. RV infection increased TLR2 expression, whereas 2’-FL boosted TLR5 and TLR7 expressions and scGOS/lcFOS increased that of TLR9. RV-infected rats displayed an intestinal dysbiosis that was effectively prevented by the dietary interventions, and consequently, their microbiota was more similar to microbiota of the noninfected groups. The preventive effect of 2’-FL, scGOS/lcFOS, and their combination on dysbiosis associated to RV diarrhea in rats could be due to changes in the crosstalk between gut microbiota and the innate immune system.
Tribological and Thermophysical Properties of Environmentally-Friendly Lubricants Based on Trimethylolpropane Trioleate with Hexagonal Boron Nitride Nanoparticles as an Additive
Authors: Liñeira del Río José M. , Guimarey María J. G. , Comuñas María J. P. , López Enriqueta R. , Prado Jose I. , Lugo Luis , Fernández Josefa
Published: August 2019

Dispersions based on hexagonal boron nitride, h-BN, nanoparticles, at 0.50, 0.75 and 1.0 wt.% mass concentrations, in an ester base oil composed mainly of trimethylolpropane trioleate, were investigated as potential nanolubricants. The stability of the dispersions was assessed to determine the reliability of the tribological, thermophysical and rheological measurements. Density and viscosity were measured from 278.15 to 373.15 K, while rheological behavior was analyzed at shear rates from 1 to 1000 s−1 at 283.15 K. Newtonian behavior was exhibited by all nanolubricants at the explored conditions, with the exception of the highest concentration at the lowest shear rates, where possible non-Newtonian behavior was observed. Tribological tests were performed under a normal load of 2.5 N. Wear was evaluated by means of a 3D profiler, scanning electron microscopy and confocal Raman microscopy. The best tribological performance was achieved by the 0.75 wt.% nanolubricant, with reductions of 25% in the friction coefficient, 9% in the scar width, 14% in the scar depth, and 22% of the transversal area, all with respect to the neat oil. It was observed that physical protective tribofilms are created between rubbing surfaces.
Research on Factors Affecting Solvers’ Participation Time in Online Crowdsourcing Contests
Authors: Yang Keng
Published: August 2019

A crowdsourcing contest is one of the most popular modes of crowdsourcing and is also an important tool for an enterprise to implement open innovation. The solvers’ active participation is one of the major reasons for the success of crowdsourcing contests. Research on solvers’ participation behavior is helpful in understanding the sustainability and incentives of solvers’ participation in the online crowdsourcing platform. So, how to attract more solvers to participate and put in more effort is the focus of researchers. In this regard, previous studies mainly used the submission quantity to measure solvers’ participation behavior and lacked an effective measure on the degree of participation effort expended by a solver. For the first time, we use solvers’ participation time as a dependent variable to measure their effort in a crowdsourcing contest. Thus, we incorporate participation time into the solver’s participation research. With the data from Taskcn.com, we analyze how participation time is affected four key factors including task design, task description, task process, and environment, respectively. We found that, first, for task design, higher task rewards will attract solvers to invest more time in the participation process and the relationship between participation time and task duration is inverted U-shaped. Second, for task description, the length of the task description has a negative impact on participation time and the task description attachment will positively influence the participation time. Third, for the task process, communication and supplementary explanations in a crowdsourcing process positively affect participation time. Fourth, for environmental factors, the task density of the crowdsourcing platform and the market price of all crowdsourcing contests have respectively negative and positive effects on participation time.
Pure Polyphenols and Cranberry Juice High in Anthocyanins Increase Antioxidant Capacity in Animal Organs
Authors: Bariexca Tracy , Ezdebski Janice , Redan Benjamin , Vinson Joe
Published: August 2019

Anthocyanins and the broader class of polyphenols are strong antioxidants in vitro. Polyphenols are one of the major antioxidants in plant foods, and the beverages derived from them. There is extensive evidence in the literature that polyphenols are beneficial to health. In order to be bioactive in vivo, they need to be bioavailable and be transported from the circulation to target organs. To date, there have been few studies testing the extent to which polyphenols and especially anthocyanins affect the antioxidant capacity of animal organs. In our first pilot study, we investigated how three pure polyphenols (the flavonoids quercetin, catechin and hesperetin) given to rats by intraperitoneal injection (49 to 63 mg/kg) affected their organ antioxidant capacity. This was followed by a subsequent study that injected one ml of 100% cranberry juice (high in anthocyanins) to hamsters. Antioxidant capacity of animal organs was determined by using the ferric reducing antioxidant power (FRAP) colorimetric assay on methanolic extracts of select rat organs (i.e., liver, kidney, heart, prostate and brain) and in the hamster organs (i.e., liver, kidney, heart, bladder and brain). Overall the results showed that antioxidant capacity was significantly increased (p < 0.05) in experimental vs. control organs. Analysis of organs by high performance liquid chromatography (HPLC) from both animal studies provided evidence of polyphenol metabolites in the organ extracts. Taken together, this study provides data that the administration of anthocyanins and other polyphenols cause an increase in organ antioxidant capacity in two animal models. This result supports the growing evidence for the hypothesis that dietary polyphenols reduce the risk and extent of various chronic disease at the disease site.
Particle Size in Secondary Particle and Magnetic Response for Carrageenan Magnetic Hydrogels
Authors: Ikeda Junko , Takahashi Daichi , Watanabe Mayuko , Kawai Mika , Mitsumata Tetsu
Published: August 2019

The relation between the number of magnetic particles and the change in storage modulus induced by a magnetic field was investigated for weak hydrogels containing carbonyl iron, iron oxide, and barium ferrite particles with different diameters in primary particles while maintaining the magnetization of magnetic particles. The change in storage modulus exhibited a power dependency against the number of magnetic particles, which was nearly independent of the magnetic particles. The change in storage modulus was successfully scaled by the reduced number of magnetic particles using the diameter of secondary particles. Microphotographs revealed that iron oxide and barium ferrite particles form aggregations while carbonyl iron particles are well dispersed in carrageenan gels. The diameter of secondary particles determined by a relation between the change in storage modulus and the reduced number of magnetic particles showed similar values with those observed in microphotographs.
Practical Tips for 3D Regional Gravity Inversion
Authors: Sampietro Daniele , Capponi Martina
Published: August 2019

To solve the inverse gravimetric problem, i.e., to estimate the mass density distribution that generates a certain gravitational field, at local or regional scale, several parameters have to be defined such as the dimension of the 3D region to be considered for the inversion, its spatial resolution, the size of its border, etc. Determining the ideal setting for these parameters is in general difficult: theoretical solutions are usually not possible, while empirical ones strongly depend on the specific target of the inversion and on the experience of the user performing the computation. The aim of the present work is to discuss empirical strategies to set these parameters in such a way to avoid distortions and errors within the inversion. In particular, the discussion is focused on the choice of the volume of the model to be inverted, the size of its boundary, its spatial resolution, and the spatial resolution of the a-priori information to be used within the data reduction. The magnitude of the possible effects due to a wrong choice of the above parameters is also discussed by means of numerical examples.
Cambrian Chordates and Vetulicolians
Authors: McMenamin Mark A. S.
Published: August 2019

Deuterostomes make a sudden appearance in the fossil record during the early Cambrian. Two bilaterian groups, the chordates and the vetulicolians, are of particular interest for understanding early deuterostome evolution, and the main objective of this review is to examine the Cambrian diversity of these two deuterostome groups. The subject is of particular interest because of the link to vertebrates, and because of the enigmatic nature of vetulicolians. Lagerstätten in China and elsewhere have dramatically improved our understanding of the range of variation in these ancient animals. Cephalochordate and vertebrate body plans are well established at least by Cambrian Series 2. Taken together, roughly a dozen chordate genera and fifteen vetulicolian genera document part of the explosive radiation of deuterostomes at the base of the Cambrian. The advent of deuterostomes near the Cambrian boundary involved both a reversal of gut polarity and potentially a two-sided retinoic acid gradient, with a gradient discontinuity at the midpoint of the organism that is reflected in the sharp division of vetulicolians into anterior and posterior sections. A new vetulicolian (Shenzianyuloma yunnanense nov. gen. nov. sp.) with a laterally flattened, polygonal anterior section provides significant new data regarding vetulicolians. Its unsegmented posterior region (‘tail’) bears a notochord and a gut trace with diverticula, both surrounded by myotome cones.
The Potential of Low-Cost UAVs and Open-Source Photogrammetry Software for High-Resolution Monitoring of Alpine Glaciers: A Case Study from the Kanderfirn (Swiss Alps)
Authors: Groos Alexander R. , Bertschinger Thalia J. , Kummer Céline M. , Erlwein Sabrina , Munz Lukas , Philipp Andreas
Published: August 2019

Unmanned Aerial Vehicles (UAV) are a rapidly evolving tool in geosciences and are increasingly deployed for studying the dynamic processes of the earth’s surface. To assess the potential of autonomous low-cost UAVs for the mapping and monitoring of alpine glaciers, we conducted multiple aerial surveys on the Kanderfirn in the Swiss Alps in 2017 and 2018 using open hardware and software of the Paparazzi UAV project. The open-source photogrammetry software OpenDroneMap was tested for the generation of high-resolution orthophotos and digital surface models (DSMs) from aerial imagery and cross-checked with the well-established proprietary software Pix4D. Accurately measured ground control points served for the determination of the geometric accuracy of the orthophotos and DSMs. A horizontal (xy) accuracy of 0.7–1.2 m and a vertical (z) accuracy of 0.7–2.1 m was achieved for OpenDroneMap, compared to a xy-accuracy of 0.3–0.5 m and a z-accuracy of 0.4–0.5 m obtained for Pix4D. Based on the analysis and comparison of different orthophotos and DSMs, surface elevation, roughness and brightness changes from 3 June to 29 September 2018 were quantified. While the brightness of the glacier surface decreased linearly over the ablation season, the surface roughness increased. The mean DSM-based elevation change across the glacier tongue was 8 m, overestimating the measured melting and surface lowering at the installed ablation stakes by about 1.5 m. The presented results highlight that self-built fixed-wing UAVs in tandem with open-source photogrammetry software are an affordable alternative to commercial remote-sensing platforms and proprietary software. The applied low-cost approach also provides great potential for other regions and geoscientific disciplines.
‘A Pause for Po-Ethics’: Seamus Heaney and the Ethics of Aesthetics
Authors: O’Brien Eugene
Published: August 2019

In this paper, I examine the connections between ethics and aesthetics in the writing of Seamus Heaney. Looking at Heaney’s neologism of ‘po-ethics’, I move through his poetry and especially his translation of Sophocles’ Philoctetes, entitled The Cure at Troy, and focus on his Fourth Irish Human Rights Commission Annual Human Rights Lecture: Writer & Righter, wherein he traces a number of strong connections between human rights workers and creative writers. The essay is written through a theoretical matrix of the ethical theories of Jacques Derrida, Emmanuel Levinas and Simon Critchley. It looks at poems from Heaney himself, as well as work from Shakespeare, Dante Alighieri, Czeslaw Miłosz, and Primo Levi. It focuses on poetic language as a discourse that can act as a counterweight and as a form of redress on behalf of the dignity of the individual human being against the pressures of mass culture and society.
Investigating Effects of Cold Water Hand Immersion on Selective Attention in Normobaric Hypoxia
Authors: Gerhart Hayden D. , Seo Yongsuk , Kim Jung-Hyun , Followay Brittany , Vaughan Jeremiah , Quinn Tyler , Gunstad John , Glickman Ellen L.
Published: August 2019

This study investigated the effect of cold-water hand immersion on selective attention as measured by the Stroop Color Word Test in nomorbaric normoxia and hypoxia. Ten healthy men rested for 60 min, after which they immersed their non-dominant hand into 5 °C water for 15 min. The interference score of the Stroop Color Word Test and thermal sensation were measured at baseline in the final 5 min of resting and in the final 5 min of cold water hand immersion. The interference score was not influenced by hypoxia but was found to be significantly improved compared to resting in both conditions during cold water hand immersion. Selective attention improved during 15 min of cold-water hand immersion, with increased thermal sensations rated as “very cool” of the immersed arm. Cold-water hand immersion may be helpful in improving cognitive function in normoxia and normobaric hypoxia.
Distribution and Potential Ecological Risk of Heavy Metals in Water, Sediments, and Aquatic Macrophytes: A Case Study of the Junction of Four Rivers in Linyi City, China
Authors: Li Xiuling , Shen Henglun , Zhao Yongjun , Cao Weixing , Hu Changwei , Sun Chen
Published: August 2019

The Yi River, the second longest river in Shandong Province, China, flows through Linyi City and is fed by three tributary rivers, Beng River, Liuqing River, and Su River in the northeastern part of the city. In this study, we determined the concentrations of five heavy metals (Cr, Ni, Cu, Zn, and Pb) in water, sediment, and aquatic macrophyte samples collected from the junction of the four rivers and evaluated the potential ecological risk of heavy metal pollution. Most of the heavy metals in water were in low concentrations with the water quality index (WQI) below 1, suggesting low metal pollution. The sediments showed low heavy metal concentrations, suggesting a low ecological risk based on the potential ecological risk index (RI) and the geo-accumulation index (Igeo). The aquatic plant species Potamogeton crispus accumulated considerable amounts of heavy metals, which were closely related to the metal concentrations of the sediment. The plant species Salvinia natans also showed an excellent metal accumulation capability. Based on our results, the junction of the four rivers is only slightly polluted in terms of heavy metals, and the plant species P. crispus is a suitable bioindicator for sediment heavy metal pollution.
Changes in Undergraduate Students’ Psychological Well-Being as They Experience University Life
Authors: Liu Xinqiao , Ping Siqing , Gao Wenjuan
Published: August 2019

The onset of most lifetime mental disorders occurs during adolescence, and the years in college, as the final stage of adolescence in a broad sense, deserve attention in this respect. The psychological well-being of undergraduate students can influence not only their academic and professional success, but also the development of society as a whole. Although previous studies suggested psychiatric disorders are common in the adult population, there was little consistent information available about undergraduate students’ mental health problems. This research aimed to describe the changes in depression, anxiety, and stress of Chinese full-time undergraduate students as they experienced university life using the Depression Anxiety Stress Scales-21 (DASS-21). The main conclusions of our study were as follows: (1) on average, students’ severity scores of depression during the four academic years varied between 7.22 and 7.79, while stress scores ranged from 9.53 to 11.68. However, the anxiety scores of college students in the first three years turned out to be 7.40, 7.24 and 7.10, respectively, slightly overtaking the normal threshold of 7. These results indicated that Chinese college students, in general, were mentally healthy with regard to depression and stress, but their average anxiety levels were beyond normal in the first three years. (2) As for the proportions of students with different degrees of severity, approximately 38% to 43% of college students were above the normal level of anxiety, about 35% above the normal level of depression, and around 20% to 30% above the normal level of stress. (3) There were significant differences in the psychological health states of students of different years, especially among the sophomores, juniors, and seniors; the highest score of depression, anxiety, and stress all appeared in the first or second year on average, but some improvements were achieved in the third and last years. The findings suggested that colleges and universities need to pay special attention to psychologically unhealthy students, and with concerted efforts by the government, formulate mental health policies in the prevention, detection, and treatment of students’ psychiatric disorders, rather than just focusing on their average levels of mental health.
Dermatologic Vasculature Diseases as a Risk Factor of Subconjunctival Hemorrhage
Authors: Lee Chia-Yi , Chen Hung-Chi , Huang Jing-Yang , Sun Chi-Chin , Yeh Chao-Bin , Lin Hung-Yu , Yang Shun-Fa
Published: August 2019

To evaluate the relationship between subconjunctival hemorrhage (SCH) and dermatologic vasculature diseases (DVDs) via the national health insurance research database (NHIRD) of Taiwan. This retrospective cohort study used data from the NHIRD for the 2009 to 2013 period. Patients diagnosed with DVDs were enrolled in the study group, and a propensity score-matching population was selected as the control group after exclusion. The main outcome was set as the development of SCH in both groups. Multivariable Cox regression analysis and survival analysis were performed to estimate the adjusted hazard ratio (aHR) and cumulative probability of SCH. A total number of 3426 patients were enrolled and split equally into the study and the control groups. There was no prominent difference between the age, gender, urbanization, income level, systemic co-morbidities, and ocular diseases between the two groups after matching. During the whole study period, 131 patients in the study group and 98 patients in the control group developed SCH with a significant higher aHR of 2.69 in the study group (p < 0.05). In the survival analysis, the study group also demonstrated a higher cumulative probability of developing SCH than the control group throughout the study period (p = 0.02). In conclusion, the presence of DVDs may be a risk factor for the development of SCH.
The Dynamic and Correlation of Skin Temperature and Cardiorespiratory Fitness in Male Endurance Runners
Authors: Galan-Carracedo Jonathan , Suarez-Segade Andrea , Guerra-Balic Myriam , Oviedo Guillermo R.
Published: August 2019

During endurance exercise, skin temperature (Tsk) plays a fundamental role in thermoregulatory processes. Environmental temperature is the biggest determinant of the Tsk. During exercise, the response of the skin temperature might be influenced by aerobic fitness (VO2peak). The aim of this study was to analyze and compare the dynamic of Tsk in high (HF) and moderately (MF) fit endurance runners during a progressive maximal stress test. Seventy-nine male endurance runners were classified into HF (n = 35; VO2peak = 56.62 ± 4.31 mL/kg/min) and MF (n = 44; VO2peak = 47.86 ± 5.29 mL/kg/min) groups. Tsk and cardiovascular data were continuously monitored during an incremental exercise, followed by a recovery period of five minutes. Results revealed that the MF group exhibited lower VO2peak, Speedpeak, ventilation (VE), muscle mass %, and higher BMI and fat mass % than the HF group (all p < 0.001). HF had significantly higher Tsk at baseline, and at 60% and 70% of peak workload (all p < 0.05). Tskpeak correlated with age, fat mass %, muscle mass %, VO2peak, Speedpeak, HR and VE (all p < 0.05). These findings indicate that VO2peak was positively associated with increased Tsk during incremental exercise in male endurance runners.
Lay People Training in CPR and in the Use of an Automated External Defibrillator, and Its Social Impact: A Community Health Study
Authors: Villalobos Felipe , Del Pozo Albert , Rey-Reñones Cristina , Granado-Font Ester , Sabaté-Lissner David , Poblet-Calaf Carme , Basora Josep , Castro Antoni , Flores-Mateo Gemma
Published: August 2019

Out-of-hospital cardiac arrest (OHCA) mortality remains high. The best survival rates are achieved when trained people provide OHCA victims with cardiopulmonary resuscitation (CPR); however, it is estimated that only 25% of victims receive CPR. This community health study aims to evaluate the effectiveness of a training programme in basic CPR and in the use of an automatic external defibrillator (AED) on knowledge and skills for lay people, and its social impact. The training courses were based on Catalan Council of Resuscitation guidelines. Data were collected on sociodemographic characteristics, evaluation of knowledge and practical skills at baseline and at the end of the training courses, and also on the social impact of the programme. A total of 36 training courses with 482 participants were carried out, and most participants achieved a qualification of suitable. The mean score in knowledge was 3.1 ± 1.1 at baseline and 3.8 ± 1.2 (p = 0.001) at the end of the programme. Participants rated the training courses as very satisfactory, considered the training useful, and felt more qualified to respond to an emergency. This study shows that a high percentage of participants acquired skills in basic CPR and use of an AED, which confirms the usefulness and effectiveness of training courses and its important social impact.
Corruption, Hidden Economy and Environmental Pollution: A Spatial Econometric Analysis Based on China’s Provincial Panel Data
Authors: Wang Shi , Yuan Yizhou , Wang Hua
Published: August 2019

Previous studies show that the environmental quality is significantly influenced by corruption and the hidden economy separately. However, what is the impact of their interaction effect on environmental quality? Based on Multiple Indicators Multiple Causes (MIMIC) model, this study calculates the scale of hidden economy in Chinese provinces firstly. Then, we apply the method of spatial econometrics to analyze the interaction effect of corruption and the hidden economy on environmental pollution with China’s provincial panel data from 1998 to 2017. The results indicate that the interaction effect between corruption and hidden economy significantly increases pollutant discharge, suggesting that both anti-corruption and control of the hidden economy may improve environmental quality directly and indirectly.
“Everyone Has the Right to Drink Beer”: A Stakeholder Analysis of Challenges to Youth Alcohol Harm-Reduction Policies in Lebanon
Authors: Nakkash Rima T , Ghandour Lilian A , Yassin Nasser , Anouti Sirine , Chalak Ali , Chehab Sara , El-Aily Aida , Afifi Rima A
Published: August 2019

Background: Alcohol use is a major risk factor in premature death and disability, especially among youth. Evidence-based policies to prevent and control the detrimental effect of alcohol use have been recommended. In countries with weak alcohol control policies—such as Lebanon, stakeholder analysis provides critical information to influence policy interventions. This paper assesses the views of stakeholders regarding a national alcohol harm reduction policy for youth. Methods: We interviewed a total of 22 key stakeholders over a period of 8 months in 2015. Stakeholders were selected purposively, to include representatives of governmental and non-governmental organizations and industry that could answer questions related to core intervention areas: affordability, availability, regulation of marketing, and drinking and driving. We analyzed interview transcripts using thematic analysis. Results: Three themes emerged: Inadequacy of current alcohol control policies; weak governance and disregard for rule of law as a determinant of the status quo; and diverting of responsibility towards ‘other’ stakeholders. In addition, industry representatives argued against evidence-based policies using time-worn strategies identified globally. Conclusions: Our findings indicate that alcohol harm reduction policies are far from becoming a policy priority in Lebanon. There is a clear need to shift the narrative from victim blaming to structural conditions.
Long-Term Trends of Liver Cancer Incidence and Mortality in China 1990–2017: A Joinpoint and Age–Period–Cohort Analysis
Authors: Wang Fang , Mubarik Sumaira , Zhang Yu , Wang Lu , Wang Yafeng , Yu Chuanhua , Li Hao
Published: August 2019

Liver cancer (LC) is one of the most common causes of cancer-related deaths: this study aims to present the long-term trends and age–period–cohort effects of the incidence of and mortality from LC in China during 1990–2017. Incidence and mortality data were obtained from the Global Burden of Disease Study 2017. We determined trends in the age-standardized incidence rate (ASIR) and mortality rate (ASMR) using Joinpoint regression. An age–period–cohort (APC) analysis was performed to describe the long-term trends with intrinsic estimator methods. The ASMR decreased markedly before 2013 and increased thereafter, with overall average annual percent change (AAPC) values of −0.5% (95% confidence interval (CI): −0.6%, −0.3%) for men and −1.3% (−1.6%, −1.0%) for women during 1990–2017. The ASIR significantly increased by 0.2% (0.1%, 0.3%) in men and decreased by 1.1% (−1.2%, −1.0%) in women from 1990 to 2017. The risks of LC incidence and mortality increased with age in both genders. The period effect risk ratios (RRs) of incidence and mortality displayed similar monotonic increasing trends in men and remained stable in women. The cohort effect showed an overall downward trend and almost overlapping incidence and mortality in both genders, and later birth cohorts experienced lower RRs than previous birth cohorts. Older age, recent period, and birth before 1923 were associated with a higher risk of liver cancer incidence and mortality. The net age and period effects showed an increasing trend, while the cohort effects presented a decreasing trend in incidence and mortality risk. As China’s population aging worsens and with the popularization of unhealthy lifestyles, the burden caused by liver cancer will remain a huge challenge in China’s future.
A Study on a Matching Algorithm for Urban Underground Pipelines
Authors: Wang Shuai , Guo Qingsheng , Xu Xinglin , Xie Yuwu
Published: August 2019

Urban underground pipelines are known as “urban blood vessels”. To detect changes in integrated pipelines and professional pipelines, the matching of same-name spatial objects is critical. Existing algorithms used for vector network matching were analyzed to develop an improved matching algorithm that can adapt to underground pipeline networks. Our algorithm improves the holistic matching of pipeline strokes, and also a partial matching algorithm is provided. In this study, appropriate geometric measures were selected to calculate the geometric similarity between pipeline strokes in their holistic matching. Existing methods for evaluating similarities in spatial scene structures in partial underground pipeline networks were improved. A method of partial matching of strokes was additionally investigated, and it compensates for the deficiencies of holistic stroke matching. Experiments showed that the matching performance was good, and the operation efficiency was high.
Mapping Spatiotemporal Data to RDF: A SPARQL Endpoint for Brussels
Authors: Vaisman Alejandro , Chentout Kevin
Published: August 2019

This paper describes how a platform for publishing and querying linked open data for the Brussels Capital region in Belgium is built. Data are provided as relational tables or XML documents and are mapped into the RDF data model using R2RML, a standard language that allows defining customized mappings from relational databases to RDF datasets. In this work, data are spatiotemporal in nature; therefore, R2RML must be adapted to allow producing spatiotemporal Linked Open Data.Data generated in this way are used to populate a SPARQL endpoint, where queries are submitted and the result can be displayed on a map. This endpoint is implemented using Strabon, a spatiotemporal RDF triple store built by extending the RDF store Sesame. The first part of the paper describes how R2RML is adapted to allow producing spatial RDF data and to support XML data sources. These techniques are then used to map data about cultural events and public transport in Brussels into RDF. Spatial data are stored in the form of stRDF triples, the format required by Strabon. In addition, the endpoint is enriched with external data obtained from the Linked Open Data Cloud, from sites like DBpedia, Geonames, and LinkedGeoData, to provide context for analysis. The second part of the paper shows, through a comprehensive set of the spatial extension to SPARQL (stSPARQL) queries, how the endpoint can be exploited.
Lessons Learned from the NOAA CoastWatch Ocean Satellite Course Developed for Integrating Oceanographic Satellite Data into Operational Use
Authors: Wilson Cara , Robinson Dale H.
Published: August 2019

Satellite data are underutilized in many branches of operational oceanography. Users outside of the satellite community often encounter difficulty in discovering the types of satellite measurements that are available, and determining which satellite products are best for operational activities. In addition, the large choice of satellite data providers, each with their own data access protocols and formats, can make data access challenging. The mission of the NOAA CoastWatch Program is to make ocean satellite data easier to access and to apply to operational uses. As part of this mission, the West Coast Node of CoastWatch developed the NOAA Ocean Satellite Course, which introduces scientists and resource managers to ocean satellite products, and provides them tools to facilitate data access when using common analysis software. These tools leverage the data services provided by ERDDAP, a data distribution system designed to make data access easier via a graphical user interface and via machine-to-machine connections. The course has been offered annually since 2006 and has been attended by over 350 participants. Results of post-course surveys are analyzed to measure course effectiveness. The lessons learned from conducting these courses include using the preferred software of the course participants, providing easy access to datasets that are appropriate (fit for purpose) for operation applications, developing tools that address common tasks of the target audience, and minimizing the financial barriers to attend the course.
The Mode-of-Action of Targeted Alpha Therapy Radium-223 as an Enabler for Novel Combinations to Treat Patients with Bone Metastasis
Authors: Suominen Mari I. , Wilson Timothy , Käkönen Sanna-Maria , Scholz Arne
Published: August 2019

Bone metastasis is a common clinical complication in several cancer types, and it causes a severe reduction in quality of life as well as lowering survival time. Bone metastases proceed through a vicious self-reinforcing cycle that can be osteolytic or osteoblastic in nature. The vicious cycle is characterized by cancer cells residing in bone releasing signal molecules that promote the differentiation of osteoclasts and osteoblasts either directly or indirectly. The increased activity of osteoclasts and osteoblasts then increases bone turnover, which releases growth factors that benefit metastatic cancer cells. In order to improve the prognosis of patients with bone metastases this cycle must be broken. Radium-223 dichloride (radium-223), the first targeted alpha therapy (TAT) approved, is an osteomimetic radionuclide that is incorporated into bone metastases where its high-linear energy transfer alpha radiation disrupts both the activity of bone cells and cancer cells. Therefore, radium-223 treatment has been shown preclinically to directly affect cancer cells in both osteolytic breast cancer and osteoblastic prostate cancer bone metastases as well as to inhibit the differentiation of osteoblasts and osteoclasts. Clinical studies have demonstrated an increase in survival in patients with metastatic castration-resistant prostate cancer. Due to the effectiveness and low toxicity of radium-223, several novel combination treatment strategies are currently eliciting considerable research interest.
Effect of Ionizing Radiation from Computed Tomography on Differentiation of Human Embryonic Stem Cells into Neural Precursors
Authors: Hanu Christine , Loeliger Burk W. , Panyutin Irina V. , Maass-Moreno Roberto , Wakim Paul , Pritchard William F. , Neumann Ronald D. , Panyutin Igor G.
Published: August 2019

We studied the effect of radiation from computed tomography (CT) scans on differentiation of human embryonic stem cells (hESCs) into neuronal lineage. hESCs were divided into three radiation exposure groups: 0-dose, low-dose, or high-dose exposure. Low dose was accomplished with a single 15 mGy CT dose index (CTDI) CT scan that approximated the dose for abdominal/pelvic CT examinations in adults while the high dose was achieved with several consecutive CT scans yielding a cumulative dose of 500 mGy CTDI. The neural induction was characterized by immunocytochemistry. Quantitative polymerase chain reaction (qPCR) and Western blots were used to measure expression of the neuronal markers PAX6 and NES and pluripotency marker OCT4. We did not find any visible morphological differences between neural precursors from irradiated and non-irradiated cells. However, quantitative analyses of neuronal markers showed that PAX6 expression was reduced following exposure to the high dose compared to 0-dose controls, while no such decrease in PAX6 expression was observed following exposure to the low dose. Similarly, a statistically significant reduction in expression of NES was observed following high-dose exposure, while after low-dose exposure, a modest but statistically significant reduction in NES expression was only observed on Day 8 of differentiation. Further studies are warranted to elucidate how lower or delayed expression of PAX6 and NES can impact human fetal brain development.
Probiotics Upregulate Trefoil Factors and Downregulate Pepsinogen in the Mouse Stomach
Authors: Khoder Ghalia , Al-Yassir Farah , Al Menhali Asma , Saseedharan Prashanth , Sugathan Subi , Tomasetto Catherine , Karam Sherif M.
Published: August 2019

Probiotics are used in the management of some gastrointestinal diseases. However, little is known about their effects on normal gastric epithelial biology. The aim of this study was to explore how the probiotic mixture VSL#3 affects gastric cell lineages in mice with a special focus on protective and aggressive factors. Weight-matching littermate male mice (n = 14) were divided into treated and control pairs. The treated mice received VSL#3 (5 mg/day/mouse) by gastric gavage for 10 days. Control mice received only the vehicle. Food consumption and bodyweight were monitored. All mice were injected intraperitoneally with bromodeoxyuridine (120 mg/Kg bodyweight) two hours before sacrificed to label S-phase cells. Stomach tissues were processed for lectin- and immunohistochemical examination. ImageJ software was used to quantify immunolabeled gastric epithelial cells. Real-time quantitative polymerase chain reaction was used to provide relative changes in expression of gastric cell lineages specific genes. Results revealed that treated mice acquired (i) increased production of mucus, trefoil factor (TFF) 1 and TFF2, (ii) decreased production of pepsinogen, and (iii) increased ghrelin-secreting cells. No significant changes were observed in bodyweight, food consumption, cell proliferation, or parietal cells. Therefore, VSL#3 administration amplifies specific cell types specialized in the protection of the gastric epithelium.
The Cyclooxigenase-2 Inhibitor Parecoxib Prevents Epidermal Dysplasia in HPV16-Transgenic Mice: Efficacy and Safety Observations
Authors: Ferreira Tiago , Campos Sandra , Silva Mónica G. , Ribeiro Rita , Santos Susana , Almeida José , Pires Maria João , Gil da Costa Rui Miguel , Córdova Cláudia , Nogueira António , Neuparth Maria João , Medeiros Rui , Monteiro Bastos Margarida Maria da Silva , Gaivão Isabel , Peixoto Francisco , Oliveira Maria Manuel , Oliveira Paula Alexandra
Published: August 2019

Carcinogenesis induced by high-risk human papillomavirus (HPV) involves inflammatory phenomena, partially mediated by cyclooxigenase-2. In pre-clinical models of HPV-induced cancer, cyclooxygenase-2 inhibitors have shown significant efficacy, but also considerable toxicity. This study addresses the chemopreventive effect and hepatic toxicity of a specific cyclooxigensase-2 inhibitor, parecoxib, in HPV16-transgenic mice. Forty-three 20 weeks-old female mice were divided into four groups: I (HPV16−/−, n = 10, parecoxib-treated); II (HPV16−/− n = 11, untreated); III (HPV16+/−, n = 11, parecoxib-treated) and IV (HPV16+/−, n = 11, untreated). Parecoxib (5.0 mg/kg once daily) or vehicle was administered intraperitoneally for 22 consecutive days. Skin lesions were classified histologically. Toxicological endpoints included genotoxic parameters, hepatic oxidative stress, transaminases and histology. Parecoxib completely prevented the onset of epidermal dysplasia in HPV16+/− treated animals (0% versus 64% in HPV16+/− untreated, p = 0.027). Parecoxib decreases lipid peroxidation (LPO) and superoxide dismutase (SOD) activity and increases the GSH:GSSG ratio in HPV16+/− treated animals meaning that oxidative stress is lower. Parecoxib increased genotoxic stress parameters in wild-type and HPV16-transgenic mice, but didn’t modify histological or biochemical hepatic parameters. These results indicate that parecoxib has chemopreventive effects against HPV16-induced lesions while maintaining an acceptable toxicological profile in this model.
The Missing Heritability of Sporadic Frontotemporal Dementia: New Insights from Rare Variants in Neurodegenerative Candidate Genes
Authors: Ciani Miriam , Bonvicini Cristian , Scassellati Catia , Carrara Matteo , Maj Carlo , Fostinelli Silvia , Binetti Giuliano , Ghidoni Roberta , Benussi Luisa
Published: August 2019

Frontotemporal dementia (FTD) is a common form of dementia among early-onset cases. Several genetic factors for FTD have been revealed, but a large proportion of FTD cases still have an unidentified genetic origin. Recent studies highlighted common pathobiological mechanisms among neurodegenerative diseases. In the present study, we investigated a panel of candidate genes, previously described to be associated with FTD and/or other neurodegenerative diseases by targeted next generation sequencing (NGS). We focused our study on sporadic FTD (sFTD), devoid of disease-causing mutations in GRN, MAPT and C9orf72. Since genetic factors have a substantially higher pathogenetic contribution in early onset patients than in late onset dementia, we selected patients with early onset (<65 years). Our study revealed that, in 50% of patients, rare missense potentially pathogenetic variants in genes previously associated with Alzheimer’s disease, Parkinson disease, amyotrophic lateral sclerosis and Lewy body dementia (GBA, ABCA7, PARK7, FUS, SORL1, LRRK2, ALS2), confirming genetic pleiotropy in neurodegeneration. In parallel, a synergic genetic effect on FTD is suggested by the presence of variants in five different genes in one single patient. Further studies employing genome-wide approaches might highlight pathogenic variants in novel genes that explain the still missing heritability of FTD.
Glucagon Control on Food Intake and Energy Balance
Authors: Al-Massadi Omar , Fernø Johan , Diéguez Carlos , Nogueiras Ruben , Quiñones Mar
Published: August 2019

Glucagon exerts pleiotropic actions on energy balance and has emerged as an attractive target for the treatment of diabetes and obesity in the last few years. Glucagon reduces body weight and adiposity by suppression of appetite and by modulation of lipid metabolism. Moreover, this hormone promotes weight loss by activation of energy expenditure and thermogenesis. In this review, we cover these metabolic actions elicited by glucagon beyond its canonical regulation of glucose metabolism. In addition, we discuss recent developments of therapeutic approaches in the treatment of obesity and diabetes by dual- and tri-agonist molecules based on combinations of glucagon with other peptides. New strategies using these unimolecular polyagonists targeting the glucagon receptor (GCGR), have become successful approaches to evaluate the multifaceted nature of glucagon signaling in energy balance and metabolic syndrome.