bluuebunny/crossref_metadata_2025 huggingface dataset:
Ivan Djaja (Jean Giaja) and the Belgrade School of Physiology
Authors: ANDJUS P. R. , STOJILKOVIC S. S. , CVIJIC G.
Published: October 2020

The founder of physiology studies in the Balkans and the pioneer of research on hypothermia, Ivan Djaja (Jean Giaja) was born 1884 in L’Havre. Giaja gained his PhD at the Sorbonne in 1909. In 1910 he established the first Chair of Physiology in the Balkans and organized the first Serbian Institute for Physiology at the School of Philosophy of the University of Belgrade. He led this Institute for more than 40 subsequent years. His most notable papers were in the field of thermoregulation and bioenergetics. Djaja became member of the Serbian and Croatian academies of science and doctor honoris causa of Sorbonne. In 1952 for the seminal work on the behavior of deep cooled warm blooded animals he became associate member of the National Medical Academy in Paris. In 1955 the French Academy of Sciences elected him as associate member in place of deceased Sir Alexander Fleming. Djaja died in 1957 during a congress held in his honor. He left more than 200 scientific and other papers and the golden DaVincian credo “Nulla dies sine experimento”. His legacy was continued by several generations of researchers, the most prominent among them being Stefan Gelineo, Radoslav Andjus and Vojislav Petrović.
Hubungan Antara Peran Perawat Sebagai Educator dengan Motivasi Sembuh Pasien Tuberkulosis di Wilayah Kerja Puskesmas Woha
Authors: - Junaidin
Published: October 2020

Tuberkulosis adalah penyakit infeksi kronis yang masih merupakan permasalahn serius yang di temukan pada penduduk dunia termasuk Indonesia. Motivasi pasien untuk sembuh mempengaruhi faktor lingkungan rumah sakit, dokter, perawat dan tenaga kesehatan lainnya serta dukungan dari keluarga Tujun dari penelitian ini adalah untuk melihat Hubungan Peran Perawat Dan Keluarga Sebagai Educator Dengan Motivasi Sembuh Pasien Tuberkulosis Di Puskesmas Woha Tahun 2019. Penelitian ini menggunakan cross sectional . populasi penelitian ini sejumlah 90 orang. Teknik pengambilan sampel menggunakan Proposive Sampling dengan jumlah sampel sebanyak 32 orang dan menggunakan Uji Chi-Square. Instrumen dalam penelitian ini menggunakan kuesioner.  Hasil analisa peran perawat diperoleh nilai pValue = 0,03 <  0,05, artinya ada hubungan peran perawat sebagai educator dengan motivasi sembuh pasien. Hasil analisa peran keluarga diperoleh nilai pValue = 0,006 <  0,05,  artinya ada hubungan peran keluarga sebagai educator dengan motivasi sembuh pasien, artinya ada hubungan peran perawat dan keluarga sebagai educator dengan motivasi sembuh pasien tuberkulosi di Puskesmas Woha Tahun 2019. Kesimpulan dari  penelitian ini adalah ada hubungan yang signifikan antara  peran perawat dan keluarga sebagai educator dengan motivasi sembuh pasien di Puskesmas Woha Tahun 2019. Untuk itu upaya pencegahan terjadinya penyakit tuberkulosis diharapkan peran perawat dan keluarga untuk selalu memberikan motivasi untuk mempercepat proses penyembuhan pasien.
KONTRIBUSI KONSEP DIRI DAN PERHATIAN ORANG TUA TERHADAP HASIL BELAJAR MATEMATIKA SISWA KELAS X SMA DI KECAMATAN WERA BIMA
Authors: Dusalan Dusalan
Published: October 2020

The purpose of this study is (1) Want to know how much the contribution of self-concept, and the attention of parents, to the results of mathematics learning in class X SMA in Wera Bima District; (2) Want to know how much the positive contribution of self-concept to the results of mathematics learning in class X SMA in Wera Bima District; and (3) Want to know how much the positive contribution of parents' attention to the results of mathematics learning in class X SMA in Wera Bima District. The type of this study was ex-post facto, the population in this study were Class X students of State High Schools in Wera Bima Subdistrict in the year 2017/2018 which were taken using Proportional stratified random sampling. Instruments used: (1) self-concept scale, (2) the scale of parental attention, (3) tests of mathematics learning outcomes. Data were analyzed by descriptive statistics and path analysis. The results of the study illustrate that; (1) Positive contribution to self-concept, and parents' attention together (simultaneous) to the mathematics learning outcomes of class X high school students in Wera Bima District, for R2square = 11.10%. (2) The positive contribution of self-concept towards the mathematics learning outcomes of class X high school students in Wera Bima Subdistrict, amounting to 15.70%. and (3) The positive contribution of parents' attention                      to the mathematics learning outcomes of class X high school students in Wera Bima District, amounting to 19.80%.
‘A disoriented vision of … fact’: Brian Friel, Francis Bacon, and Faith Healer
Authors: Kuczyńska Zosia
Published: October 2020

The Brian Friel Papers at the NLI reveal a long and relatively unexplored history of major and minor influences on Friel's plays. As the archive attests, these influences manifest themselves in ways that range from the superficial to the deeply structural. In this article, I draw on original archival research into the composition process of Friel's genre-defining play Faith Healer (1979) to bring to light a model of influence that operates at the level of artistic practice. Specifically, I examine the extent to which Friel's officially unacknowledged encounter with a book of interviews with painter Francis Bacon influenced the play in terms of character, language, and form. I suggest that Bacon's creative process – incorporating his ideas on the role of the artist, the workings of chance, and the extent to which art does violence to fact – may have had a major influence on both the play's development and on Friel's development as an artist.
The People vs the NHS: Biosexual Citizenship and Hope in Stories of PrEP Activism
Authors: Jones Charlotte , Young Ingrid , Boydell Nicola
Published: October 2020

Discourses of pre-exposure prophylaxis (PrEP) revel in its radical potential as a global HIV prevention technology, offering a promise of change for the broader landscape of HIV prevention. In 2018, the British Broadcasting Corporation (BBC) aired The People vs The NHS: Who Gets the Drugs?, a documentary focused on the ‘battle’ to make PrEP available in England. In this article we explore how the BBC documentary positions PrEP, PrEP biosexual citizen-activists, as well as the wider role of the NHS in HIV prevention and the wellbeing of communities affected by HIV in the UK. We consider how biosexual citizenship ( Epstein 2018 ) is configured through future imaginaries of hope, and the spectral histories of AIDS activism. We describe how The People crafts a story of PrEP activism in the context of an imagined gay community whose past, present, and hopeful future is entangled within the complexities and contractions of a state-funded health system. Here, PrEP functions as a ‘happiness pointer’ ( Ahmed 2011 ), to orient imagined gay communities towards a hopeful future by demanding and accessing essential medicines and ensuring the absence of needless HIV transmissions. This biomedical success emerges from a shared traumatic past and firmly establishes the salvatory trajectory of PrEP and an imagined gay community who have continued to be affected by HIV. However, campaigns about the individual's right to access PrEP construct the availability and consumption of PrEP as an end goal to their activism, where access to PrEP is understood as an individual's right as a pharmaceutical consumer.
Differential Expression Analysis Revealing CLCA1 to Be a Prognostic and Diagnostic Biomarker for Colorectal Cancer
Authors: Wei Fang-Ze , Mei Shi-Wen , Wang Zhi-Jie , Chen Jia-Nan , Shen Hai-Yu , Zhao Fu-Qiang , Li Juan , Liu Zheng , Liu Qian
Published: October 2020

Colorectal cancer (CRC) is a common malignant tumor of the digestive tract and lacks specific diagnostic markers. In this study, we utilized 10 public datasets from the NCBI Gene Expression Omnibus (NCBI-GEO) database to identify a set of significantly differentially expressed genes (DEGs) between tumor and control samples and WGCNA (Weighted Gene Co-Expression Network Analysis) to construct gene co-expression networks incorporating the DEGs from The Cancer Genome Atlas (TCGA) and then identify genes shared between the GEO datasets and key modules. Then, these genes were screened via MCC to identify 20 hub genes. We utilized regression analyses to develop a prognostic model and utilized the random forest method to validate. All hub genes had good diagnostic value for CRC, but only CLCA1 was related to prognosis. Thus, we explored the potential biological value of CLCA1. The results of gene set enrichment analysis (GSEA) and immune infiltration analysis showed that CLCA1 was closely related to tumor metabolism and immune invasion of CRC. These analysis results revealed that CLCA1 may be a candidate diagnostic and prognostic biomarker for CRC.
A Dynamic Anomaly Detection Approach Based on Permutation Entropy for Predicting Aging-Related Failures
Authors: Wang Shuguang , Lu Minyan , Kong Shiyi , Ai Jun
Published: October 2020

Software aging is a phenomenon referring to the performance degradation of a long-running software system. This phenomenon is an accumulative process during execution, which will gradually lead the system from a normal state to a failure-prone state. It is a crucial challenge for system reliability to predict the Aging-Related Failures (ARFs) accurately. In this paper, permutation entropy (PE) is modified to Multidimensional Multi-scale Permutation Entropy (MMPE) as a novel aging indicator to detect performance anomalies, since MMPE is sensitive to dynamic state changes. An experiment is set on the distributed database system Voldemort, and MMPE is calculated based on the collected performance metrics during execution. Finally, based on MMPE, a failure prediction model using the machine learning method to reveal the anomalies is presented, which can predict failures with high accuracy.
An Exploratory Economic Analysis of Underground Pumped-Storage Hydro Power Plants in Abandoned Deep Coal Mines
Authors: Madlener Reinhard , Specht Jan Martin
Published: October 2020

This study researches the concept of underground pumped-storage hydro power plants in closed-down underground hard coal mines in Germany. After a review on how this could be realized technically, an economic feasibility analysis is presented, with a particular focus on the costs for the underground storage reservoir. The analysis is performed for different lower (i.e., underground) reservoir sizes and temporal arbitrage potentials (peak/off-peak electricity price spreads), and cost uncertainty is dealt with by means of a Monte Carlo simulation for two distinct head heights. The findings regarding costs and acceptability are compared with those of a classic (on-surface) pumped-storage hydro power plant in a mountainous area. Based on a techno-economic evaluation we conclude that under favorable conditions the realization of underground pumped-storage hydro power (UPSHP) plants seems both technically feasible and economically reasonable. More specifically, an extension of a tubular system seems the most promising option. A UPSHP plant in a mineshaft is probably slightly more expensive than a conventional one, an outcome that depends strongly on the feasible head height. However, the significant reduction of the adverse impacts on the landscape and on local residents, as well as a potentially large number of feasible sites in flat terrain, could make UPSHPs an interesting option for the future energy transition, not just in Germany but worldwide at sites where underground mining is being abandoned.
An Analysis of Support Mechanisms for New CHPs: The Case of Poland
Authors: Zamasz Krzysztof , Kapłan Radosław , Kaszyński Przemysław , Saługa Piotr W.
Published: October 2020

The increasing demand for energy on a global scale, as well as the social pressure related to counteracting the effects of climate change, has created favourable conditions for the transformation of energy sectors towards the possession of low-emission generation sources. This situation, however, requires investment actions in order to modernise the existing power and CHP (Combined Heat and Power) plants and construct new units. These issues, together with the climate and energy policy pursued by the European Union, are the main reasons for the emergence of various governmental mechanisms supporting the replacement of old coal power units with highly efficient cogeneration units based on gas turbines and other units. The support may take different forms. This article discusses two examples of mechanisms available on the Polish market, i.e., (i) the capacity market and (ii) promoting electricity from high-efficiency cogeneration in the form of individual cogeneration premium. The purpose and novelty of the analysis was to identify the pros and cons and the key parameters which determine the advantage of a given mechanism. Both these mechanisms have been characterised and then compared via the example of a planned cogeneration gas unit (an open cycle gas turbine—OCGT). This assessment was made using discount methods based on the FCFF (free cashflow to company) approach. The analysis did not bring forward an unequivocal answer as to the absolute advantage of any of the solutions, but it was able to point out significant problems related to their practical use.
Moose Browsing Tends Spruce Plantations More Efficiently Than a Single Mechanical Release
Authors: De Vriendt Laurent , Thiffault Nelson , Royo Alejandro A. , Barrette Martin , Tremblay Jean-Pierre
Published: October 2020

Forest vegetation management can improve planted seedling survival and growth and is thus widely used in plantation silviculture. In some jurisdictions, mechanical release using brushsaws has replaced the traditional use of chemical herbicides for forest vegetation management purposes. However, its associated costs and the increasing difficulty of finding qualified labor represent a challenge. The browsing of competition by large herbivores may represent an alternative to mechanical release when planted seedlings are resistant to browsing. Here, we compare the efficacy of moose browsing relative to mechanical release in controlling competing vegetation and in promoting white spruce growth in plantations. In a high moose density region, we used an experimental design consisting of four pairs of moose exclosures and unfenced plots; fifty percent of both the access-restricted and unrestricted study areas received a mechanical release treatment. Moose browsing was more efficient than mechanical release in diminishing the sapling density and basal area of competing species. Mechanical release only reduced the sapling density of taller competitors (height > 201 cm), whereas browsing reduced the sapling densities of competitors across a greater size range (height > 130 cm). These effects of moose browsing on competition translated into a greater positive effect of moose browsing on the basal area of planted spruces. We attribute the higher effectiveness of moose browsing relative to mechanical release to its chronic nature. Moose browsed continuously throughout the year and for multiple years, whereas mechanical release was applied only one time between the second and fourth years after planting. Our results suggest that pairing wildlife management and silviculture decisions could be in the best interest of both the hunting and forestry industries in regions where plantations are frequent and use browse-resistant crop trees. Favouring browsers in controlling the density of competing species could increase the hunting experience and income, while providing an effective, cost-free, and socially acceptable forest vegetation management service.
Earthen Architecture in Greece: Traditional Techniques and Revaluation
Authors: Mousourakis Αpostolos , Arakadaki Maria , Kotsopoulos Sofoklis , Sinamidis Iordanis , Mikrou Tina , Frangedaki Evangelia , Lagaros Nikos D.
Published: October 2020

A big part of traditional architecture both in rural and urban areas in the Greek territory has been built with raw earth. The aim of this paper is to present earthen buildings’ constructions in Greece and show their important contribution to our heritage. The use of earth as a basic constructing material has given different earthen building cultures and techniques. Earthen construction encloses many varied uses and applications, as walls or as plasters. In different periods of time and historical contexts, from the indigenous inhabitants to the neighborhoods of the refugees of Asia Minor Catastrophe, the earth constructions had a primary role. The existence of earthen architecture was investigated in urban and rural sites in Greece. Building information, documentation, and records of buildings’ design, construction techniques, elements, and systems are presented. Today, there is still a rich architectural heritage throughout the country, which has lasted through the years and withstood seismic activities and poor conservation.
Motivation for and Effect of Cooking Class Participation: A Cross-Sectional Study Following the 2011 Great East Japan Earthquake and Tsunami
Authors: Tashiro Ai , Sakisaka Kayako , Kinoshita Yuri , Sato Kanako , Hamanaka Sakiko , Fukuda Yoshiharu
Published: October 2020

We explored the association between the motivation for and effects of cooking class participation in disaster-affected areas following the 2011 Great East Japan Earthquake and Tsunami. We conducted questionnaire surveys in January and February 2020, and applied three Poisson regression models to a cross-sectional dataset of participants, analyzing three perceived participation effects: increase in new acquaintances and friends, increase in excursion opportunities, potential for gaining motivation, and a new sense of life purpose. We also applied the interaction term of motivation variables and usual eating patterns (eating alone or with others). We obtained 257 valid responses from 15 cooking venues. The interaction term for participants’ motivation and eating patterns was associated with their perceived participation effects. “Motivation for nutrition improvement × eating alone” was positively associated with an increase in new acquaintances and friends (IRR: 3.05, 95% CI, 1.22–7.64). “Motivation for increasing personal cooking repertoire × eating alone” was positively associated with increased excursion opportunities (IRR: 5.46, 95% CI, 1.41–21.20). In contrast, the interaction effect of “motivation of increasing nutrition improvement × eating alone” was negatively associated with increased excursion opportunities (IRR: 0.27, 95% CI, 0.12–0.69). The results show that the cooking class was effective, as residents’ participation improved their nutritional health support and increased their social relationships.
Knowledge, Attitudes and Practices Towards COVID-19 Amongst Ethnic Minorities in Hong Kong
Authors: Wong Cho Lee , Chen Jieling , Chow Ka Ming , Law Bernard M.H. , Chan Dorothy N.S. , So Winnie K.W. , Leung Alice W.Y. , Chan Carmen W.H.
Published: October 2020

This study assessed the knowledge, attitudes and practices (KAP) towards coronavirus disease 2019 (COVID-19) among South Asians in Hong Kong and examined the factors that affect KAP towards COVID-19 in this population. This cross-sectional descriptive study recruited participants with assistance from South Asian community centres and organisations. A total of 352 participants completed questionnaires to assess their level of KAP towards COVID-19. The mean knowledge score was 5.38/10, indicating a relatively low knowledge level. The participants expressed certain misconceptions regarding the prevention of COVID-19 infection. They perceived a mild risk related to the disease, had positive attitudes regarding its prevention and often implemented recommended disease-preventive measures, such as maintaining social distance (88.1%) and wearing masks in public (94.3%). Participants who were male, had a secondary school education or lower and who perceived a lower risk of being infected and lower self-efficacy were less likely to implement preventive measures. Culturally and linguistically appropriate health education could be developed to increase the knowledge of South Asians, especially those with lower education levels, about COVID-19 and to encourage them to implement the necessary preventive measures.
Prevalence and Associated Factors of Self-Treatment among the Elderly—A Comparative Study between Empty and Non-Empty Nesters in Shandong, China
Authors: Gao Zhaorong , Xu Lingzhong , Qin Wenzhe , Zhang Jiao , Zhou Jinling , Hu Fangfang , Hong Zhuang
Published: October 2020

(1) Objectives: With an aging society in China, self-treatment now plays an important role in health care among older adults, but it can be problematic. This study aims to explore and compare the self-treatment behavior among empty and non-empty nesters. (2) Methods: Using a multi-stage stratified random cluster sampling method, a total of 4366 elderly people aged 60 and above from Shandong Province, China, were enrolled in this study. Data were collected through a structured questionnaire. Binary logistic regression was used to analyze the associated factors of self-treatment. (3) Results: The prevalence of self-treatment in empty nesters was significantly lower than that in non-empty ones (74.0% vs. 83.3%). Binary logistic regression analysis showed that higher educational level and poorer self-rated economic status were negatively associated with self-treatment in empty nesters, while unemployed and urban and rural residents’ basic medical insurance were positively associated with self-treatment in non-empty ones. (4) Conclusions: The study indicated that empty nesters had lower likelihood of self-treatment than non-empty ones. Empty nesters with better socioeconomic status were more likely to use self-treatment; by contrast, non-empty nesters with relatively poorer socioeconomic status were more inclined to self-treatment. Targeted interventions should be developed to maximize the effectiveness of self-treatment and reduce health risks.
Effectiveness of Hydrocolloid Dressings for Treating Pressure Ulcers in Adult Patients: A Systematic Review and Meta-Analysis
Authors: Kamińska Magdalena Sylwia , Cybulska Anna Maria , Skonieczna-Żydecka Karolina , Augustyniuk Katarzyna , Grochans Elżbieta , Karakiewicz Beata
Published: October 2020

The aim of this study was to assess the effectiveness of hydrocolloid dressings in the treatment of grade I, II, III, and IV pressure ulcers in adult patients. We compared the therapeutic effects of hydrocolloids and alternative dressings in pressure ulcer treatment. We conducted a systematic review, using a literature search only in English, from database inception until 20 April 2020, to identify randomized trials comparing various types of dressings applied in the healing of pressure ulcers. The databases were PubMed, Embase, and Cumulative Index to Nursing and Allied Health Literature (CINAHL). The study selection was performed independently by two reviewers. Data were extracted based on the guidelines included in the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) protocol. The risk of bias in the included studies was assessed using a standardized critical appraisal instrument developed by the Cochrane Collaboration. Random-effect meta-analysis of data from three or more studies was performed using meta-analysis software (Comprehensive Meta-Analysis V3, Biostat, New Jersey, USA). A total of 1145 records were identified, of which 223 were qualified after further verification, of which eight were finally included in further analysis. Hydrocolloid dressings were not superior to control therapeutics (p = 0.839; Z = 0.203; CI 95%: 0.791–1.334). They were not associated with higher healing rates (p = 0.718; Z = 0.361; OR: 0.067; CI 95%: 0.297–0.431), nor did they decrease the incidence of adverse events compared with control therapeutics (p = 0.300; Z = −1.036; OR: 0.067; CI 95%: 0.394–1.333). In the above cases, Egger’s test also did not indicate publication bias (t value = 0.779, p = 0.465; t value = 1.198, p = 0.442; t value = 0.834, p = 0.465, respectively). The present meta-analysis shows that hydrocolloid dressings are not significantly better than alternative ones in the healing of pressure ulcers in adult patients.
Prevalence of Physical Frailty and Its Multidimensional Risk Factors in Korean Community-Dwelling Older Adults: Findings from Korean Frailty and Aging Cohort Study
Authors: Jung Heeeun , Kim Miji , Lee Yunhwan , Won Chang Won
Published: October 2020

Frailty is defined as a state of increased vulnerability to stressors, and it predicts disability and mortality in the older population. This study aimed to investigate the standardized prevalence and multidimensional risk factors associated with frailty among Korean community-dwelling older adults. We analyzed the baseline data of 2907 adults aged 70–84 years (mean age 75.8 ± 3.9 years, 57.8% women) in the Korean Frailty and Aging Cohort Study. The Fried frailty phenotype was used to define frailty. Analyzed data included sociodemographic, physical, physical function, biological, lifestyle, health condition, medical condition, psychological, and social domains. Data were standardized using the national standard population composition ratio based on the Korean Population and Housing Census. The standardized prevalence of frailty and prefrailty was 7.9% (95% confidence interval (CI) 6.8–8.9%) and 47.0% (95% CI, 45.1–48.8%), respectively. The following 14 risk factors were significantly associated with frailty: at risk of malnutrition, sarcopenia, severe mobility limitation, poor social capital, rural dwellers, depressive symptoms, poor self-perceived health, polypharmacy, elevated high-sensitivity C-reactive protein, elevated glycosylated hemoglobin, low 25-hydroxy vitamin D level, longer Timed Up and Go, and low Short Physical Performance Battery score (p < 0.05). Physiconutritional, psychological, sociodemographic, and medical factors are strongly associated with frailty.
Acceptance and Potential Impact of the eWALL Platform for Health Monitoring and Promotion in Persons with a Chronic Disease or Age-Related Impairment
Authors: Infarinato Francesco , Jansen-Kosterink Stephanie , Romano Paola , van Velsen Lex , op den Akker Harm , Rizza Federica , Ottaviani Marco , Kyriazakos Sofoklis , Wais-Zechmann Beatrix , Garschall Markus , Bonassi Stefano , Hermens Hermie J.
Published: October 2020

Pervasive health technologies can increase the effectiveness of personal health monitoring and training, but more user studies are necessary to understand the interest for these technologies, and how they should be designed and implemented. In the present study, we evaluated eWALL, a user-centered pervasive health technology consisting of a platform that monitors users’ physical and cognitive behavior, providing feedback and motivation via an easy-to-use, touch-based user interface. The eWALL was placed for one month in the home of 48 subjects with a chronic condition (chronic obstructive pulmonary disease—COPD or mild cognitive impairment—MCI) or with an age-related impairment. User acceptance, platform use, and potential clinical effects were evaluated using surveys, data logs, and clinical scales. Although some features of the platform need to be improved before reaching technical maturity and making a difference in patients’ lives, the real-life evaluation of eWALL has shown how some features may influence patients’ intention to use this promising technology. Furthermore, this study made it clear how the free use of different health apps is modulated by the real needs of the patient and by their usefulness in the context of the patient’s clinical status.
Analysis of Labial and Lingual Strength among Healthy Chinese Adults in Taiwan
Authors: Wu Shang-Jung , Wang Chun-Chieh , Lin Feng-Yu , Tseng Kai-Yu , Hwu Yueh-Juen
Published: October 2020

This study collected 11 parameters regarding the labial and lingual strength for maximum isometric and swallowing tasks among 150 healthy Chinese adults in Taiwan. Measurements were performed using the Iowa Oral Performance Instrument (IOPI). All of the labial and lingual strength parameters were measured three times. The maximal value of three trials represents the pressure of every parameter. The overall mean (±standard deviation) and maximum isometric pressures of the lips, anterior tongue, and posterior tongue were 24.81 ± 5.64, 55.95 ± 14.13, and 53.23 ± 12.24 kPa, respectively. The mean value of posterior tongue strength was less than that of the anterior tongue by approximately 5%. The percentages of maximum isometric tongue pressure during the swallowing of saliva and water were 85% and 80% for the anterior tongue and 90% and 81% for the posterior tongue, respectively. The average endurances for the anterior tongue and posterior tongue were 13.86 ± 7.08 and 10.06 ± 5.40 s, respectively. The maximum isometric pressures were greater than both the saliva and water swallowing pressures, and the saliva swallowing pressures were greater than the water swallowing pressures. A value of 33 kPa in maximum isometric pressure could serve as a demarcation of weak tongue strength for healthy Chinese adults. As for the repeated trials of labial and lingual strength, there were no statistically significant differences for any of the pressures obtained from the 11 labial and lingual strength parameters. The normative data can be used for the objective assessment of labial and lingual strength in healthy Chinese adults.
Surveillance of Zoonotic Parasites in Animals Involved in Animal-Assisted Interventions (AAIs)
Authors: Simonato Giulia , Danesi Patrizia , Frangipane di Regalbono Antonio , Dotto Giorgia , Tessarin Cinzia , Pietrobelli Mario , Pasotto Daniela
Published: October 2020

Animal-assisted interventions (AAIs) are based on the establishment of a therapeutic relationship between animals and beneficiaries that is certain to provide positive effects, while currently, it reads as if AAIs aim at exposing stakeholders to potential risk of infection. The surveillance of zoonotic pathogens is necessary for guaranteeing common health. This study investigated the presence of potentially zoonotic parasites, including dermatophytes, in animals involved in AAIs. Between 2015 and 2017, 190 animals (equids, dogs, cats, birds, rabbits, rodents, and goats) were investigated. Anamnestic and management data were recorded. Individual faecal samples were analysed using a copromicroscopic procedure. Fur and skin were examined for ectoparasites during clinical examinations, and samples for mycological investigation were collected by brushing. Parasites were described in 60 (31.6%) investigated animals. Thirteen out of the 60 (21.7%) animals harboured potentially zoonotic parasites, mainly recovered in dogs (Ancylostomatidae, Eucoleus aerophilus, Toxocara canis, and Giardia duodenalis) and a cat (G. duodenalis). Nannizzia gypsea and Paraphyton mirabile, potential agents of cutaneous mycosis, were isolated in a dog and a horse, respectively. No ectoparasites were found. AAIs might represent a source of infections either directly or via environmental contamination. Thus, active surveillance is necessary and animal screenings should be planned and scheduled according to the risk of exposure.
Optimising Web-Based Computer-Tailored Physical Activity Interventions for Prostate Cancer Survivors: A Randomised Controlled Trial Examining the Impact of Website Architecture on User Engagement
Authors: Finlay Amy , Evans Holly , Vincent Andrew , Wittert Gary , Vandelanotte Corneel , Short Camille E
Published: October 2020

Background: Web-based computer-tailored interventions can assist prostate cancer survivors to become more physically active by providing personally relevant behaviour change support. This study aimed to explore how changing the website architecture (free choice vs. tunnelled) impacted engagement within a physical activity computer-tailored intervention targeting prostate cancer survivors. Methods: On a 2:2:1 ratio, 71 Australian prostate cancer survivors with local or locally advanced disease (mean age: 66.6 years ± 9.66) were randomised into either a free-choice (N = 27), tunnelled (N = 27) or minimal intervention control arm (N =17). The primary outcome was differences in usage of the physical activity self-monitoring and feedback modules between the two intervention arms. Differences in usage of other website components between the two intervention groups were explored as secondary outcomes. Further, secondary outcomes involving comparisons between all study groups (including the control) included usability, personal relevance, and behaviour change. Results: The average number of physical activity self-monitoring and feedback modules accessed was higher in the tunnelled arm (M 2.6 SD 1.3) compared to the free-choice arm (M 1.5 SD 1.4), p = 0.01. However, free-choice participants were significantly more likely to have engaged with the social support (p = 0.008) and habit formation (p = 0.003) ‘once-off’ modules compared to the standard tunnelled arm. There were no other between-group differences found for any other study outcomes. Conclusion: This study indicated that website architecture influences behavioural engagement. Further research is needed to examine the impact of differential usage on mechanisms of action and behaviour change.
‘Candidatus Liberibacter Asiaticus’ SDE1 Effector Induces Huanglongbing Chlorosis by Downregulating Host DDX3 Gene
Authors: Zhou Yinghui , Wei Xiangying , Li Yanjiao , Liu Zhiqin , Duan Yongping , Zou Huasong
Published: October 2020

‘Candidatus Liberibacter asiaticus’ (CLas) is the pathogenic bacterium that causes the disease Huanglongbing (HLB) in citrus and some model plants, such as Nicotiana benthamiana. After infection, CLas releases a set of effectors to modulate host responses. One of these critical effectors is Sec-delivered effector 1 (SDE1), which induces chlorosis and cell death in N. benthamiana. In this study, we revealed the DEAD-box RNA helicase (DDX3) interacts with SDE1. Gene silencing study revealed that knockdown of the NbDDX3 gene triggers leaf chlorosis, mimicking the primary symptom of CLas infection in N. benthamiana. The interactions between SDE1 and NbDDX3 were localized in the cell membrane. Overexpression of SDE1 resulted in suppression of NbDDX3 gene expression in N. benthamiana, which suggests a critical role of SDE1 in modulating NbDDX3 expression. Furthermore, we verified the interaction of SDE1 with citrus DDX3 (CsDDX3), and demonstrated that the expression of the CsDDX3 gene was significantly reduced in HLB-affected yellowing and mottled leaves of citrus. Thus, we provide molecular evidence that the downregulation of the host DDX3 gene is a crucial mechanism of leaf chlorosis in HLB-affected plants. The identification of CsDDX3 as a critical target of SDE1 and its association with HLB symptom development indicates that the DDX3 gene is an important target for gene editing, to interrupt the interaction between DDX3 and SDE1, and therefore interfere host susceptibility.
The Status of Soil Microbiome as Affected by the Application of Phosphorus Biofertilizer: Fertilizer Enriched with Beneficial Bacterial Strains
Authors: Mącik Mateusz , Gryta Agata , Sas-Paszt Lidia , Frąc Magdalena
Published: October 2020

Regarding the unfavourable changes in agroecosystems resulting from the excessive application of mineral fertilizers, biopreparations containing live microorganisms are gaining increasing attention. We assumed that the application of phosphorus mineral fertilizer enriched with strains of beneficial microorganisms contribute to favourable changes in enzymatic activity and in the genetic and functional diversity of microbial populations inhabiting degraded soils. Therefore, in field experiments conditions, the effects of phosphorus fertilizer enriched with bacterial strains on the status of soil microbiome in two chemically degraded soil types (Brunic Arenosol—BA and Abruptic Luvisol—AL) were investigated. The field experiments included treatments with an optimal dose of phosphorus fertilizer (without microorganisms—FC), optimal dose of phosphorus fertilizer enriched with microorganisms including Paenibacillus polymyxa strain CHT114AB, Bacillus amyloliquefaciens strain AF75BB and Bacillus sp. strain CZP4/4 (FA100) and a dose of phosphorus fertilizer reduced by 40% and enriched with the above-mentioned bacteria (FA60). The analyzes performed included: the determination of the activity of the soil enzymes (protease, urease, acid phosphomonoesterase, β-glucosidase), the assessment of the functional diversity of microorganisms with the application of BIOLOGTM plates and the characterization of the genetic diversity of bacteria, archaea and fungi with multiplex terminal restriction fragment length polymorphism and next generation sequencing. The obtained results indicated that the application of phosphorus fertilizer enriched with microorganisms improved enzymatic activity, and the genetic and functional diversity of the soil microbial communities, however these effects were dependent on the soil type.
Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration
Authors: Esteca Marcos V. , Severino Matheus B. , Silvestre João G. , Palmeira dos Santos Gustavo , Tamborlin Letícia , Luchessi Augusto D. , Moriscot Anselmo S. , Gustafsson Åsa B. , Baptista Igor L.
Published: October 2020

The high capacity of the skeletal muscle to regenerate is due to the presence of muscle stem cells (MuSCs, or satellite cells). The E3 ubiquitin ligase Parkin is a key regulator of mitophagy and is recruited to mitochondria during differentiation of mouse myoblast cell line. However, the function of mitophagy during regeneration has not been investigated in vivo. Here, we have utilized Parkin deficient (Parkin–/–) mice to investigate the role of Parkin in skeletal muscle regeneration. We found a persistent deficiency in skeletal muscle regeneration in Parkin–/– mice after cardiotoxin (CTX) injury with increased area of fibrosis and decreased cross-sectional area (CSA) of myofibres post-injury. There was also a significant modulation of MuSCs differentiation and mitophagic markers, with altered mitochondrial proteins during skeletal muscle regeneration in Parkin–/– mice. Our data suggest that Parkin-mediated mitophagy plays a key role in skeletal muscle regeneration and is necessary for MuSCs differentiation.
Manipulation of Axonal Outgrowth via Exogenous Low Forces
Authors: De Vincentiis Sara , Falconieri Alessandro , Scribano Vincenzo , Ghignoli Samuele , Raffa Vittoria
Published: October 2020

Neurons are mechanosensitive cells. The role of mechanical force in the process of neurite initiation, elongation and sprouting; nerve fasciculation; and neuron maturation continues to attract considerable interest among scientists. Force is an endogenous signal that stimulates all these processes in vivo. The axon is able to sense force, generate force and, ultimately, transduce the force in a signal for growth. This opens up fascinating scenarios. How are forces generated and sensed in vivo? Which molecular mechanisms are responsible for this mechanotransduction signal? Can we exploit exogenously applied forces to mimic and control this process? How can these extremely low forces be generated in vivo in a non-invasive manner? Can these methodologies for force generation be used in regenerative therapies? This review addresses these questions, providing a general overview of current knowledge on the applications of exogenous forces to manipulate axonal outgrowth, with a special focus on forces whose magnitude is similar to those generated in vivo. We also review the principal methodologies for applying these forces, providing new inspiration and insights into the potential of this approach for future regenerative therapies.
NAA10 as a New Prognostic Marker for Cancer Progression
Authors: Kim Sun Myung , Ha Eunyoung , Kim Jinyoung , Cho Chiheum , Shin So-Jin , Seo Ji Hae
Published: October 2020

N-α-acetyltransferase 10 (NAA10) is an acetyltransferase that acetylates both N-terminal amino acid and internal lysine residues of proteins. NAA10 is a crucial player to regulate cell proliferation, migration, differentiation, apoptosis, and autophagy. Recently, mounting evidence presented the overexpression of NAA10 in various types of cancer, including liver, bone, lung, breast, colon, and prostate cancers, and demonstrated a correlation of overexpressed NAA10 with vascular invasion and metastasis, thereby affecting overall survival rates of cancer patients and recurrence of diseases. This evidence all points NAA10 toward a promising biomarker for cancer prognosis. Here we summarize the current knowledge regarding the biological functions of NAA10 in cancer progression and provide the potential usage of NAA10 as a prognostic marker for cancer progression.
Hyperthermia Induced by Gold Nanoparticles and Visible Light Photothermy Combined with Chemotherapy to Tackle Doxorubicin Sensitive and Resistant Colorectal Tumor 3D Spheroids
Authors: Roma-Rodrigues Catarina , Pombo Inês , Fernandes Alexandra R. , Baptista Pedro V.
Published: October 2020

Current cancer therapies are frequently ineffective and associated with severe side effects and with acquired cancer drug resistance. The development of effective therapies has been hampered by poor correlations between pre-clinical and clinical outcomes. Cancer cell-derived spheroids are three-dimensional (3D) structures that mimic layers of tumors in terms of oxygen and nutrient and drug resistance gradients. Gold nanoparticles (AuNP) are promising therapeutic agents which permit diminishing the emergence of secondary effects and increase therapeutic efficacy. In this work, 3D spheroids of Doxorubicin (Dox)-sensitive and -resistant colorectal carcinoma cell lines (HCT116 and HCT116-DoxR, respectively) were used to infer the potential of the combination of chemotherapy and Au-nanoparticle photothermy in the visible (green laser of 532 nm) to tackle drug resistance in cancer cells. Cell viability analysis of 3D tumor spheroids suggested that AuNPs induce cell death in the deeper layers of spheroids, further potentiated by laser irradiation. The penetration of Dox and earlier spheroid disaggregation is potentiated in combinatorial therapy with Dox, AuNP functionalized with polyethylene glycol (AuNP@PEG) and irradiation. The time point of Dox administration and irradiation showed to be important for spheroids destabilization. In HCT116-sensitive spheroids, pre-irradiation induced earlier disintegration of the 3D structure, while in HCT116 Dox-resistant spheroids, the loss of spheroid stability occurred almost instantly in post-irradiated spheroids, even with lower Dox concentrations. These results point towards the application of new strategies for cancer therapeutics, reducing side effects and resistance acquisition.
Wnt Pathway: An Integral Hub for Developmental and Oncogenic Signaling Networks
Authors: Sharma Monica , Pruitt Kevin
Published: October 2020

The Wnt pathway is an integral cell-to-cell signaling hub which regulates crucial development processes and maintenance of tissue homeostasis by coordinating cell proliferation, differentiation, cell polarity, cell movement, and stem cell renewal. When dysregulated, it is associated with various developmental diseases, fibrosis, and tumorigenesis. We now better appreciate the complexity and crosstalk of the Wnt pathway with other signaling cascades. Emerging roles of the Wnt signaling in the cancer stem cell niche and drug resistance have led to development of therapeutics specifically targeting various Wnt components, with some agents currently in clinical trials. This review highlights historical and recent findings on key mediators of Wnt signaling and how they impact antitumor immunity and maintenance of cancer stem cells. This review also examines current therapeutics being developed that modulate Wnt signaling in cancer and discusses potential shortcomings associated with available therapeutics.
QSAR Implementation for HIC Retention Time Prediction of mAbs Using Fab Structure: A Comparison between Structural Representations
Authors: Karlberg Micael , de Souza João Victor , Fan Lanyu , Kizhedath Arathi , Bronowska Agnieszka K. , Glassey Jarka
Published: October 2020

Monoclonal antibodies (mAbs) constitute a rapidly growing biopharmaceutical sector. However, their growth is impeded by high failure rates originating from failed clinical trials and developability issues in process development. There is, therefore, a growing need for better in silico tools to aid in risk assessment of mAb candidates to promote early-stage screening of potentially problematic mAb candidates. In this study, a quantitative structure–activity relationship (QSAR) modelling workflow was designed for the prediction of hydrophobic interaction chromatography (HIC) retention times of mAbs. Three novel descriptor sets derived from primary sequence, homology modelling, and atomistic molecular dynamics (MD) simulations were developed and assessed to determine the necessary level of structural resolution needed to accurately capture the relationship between mAb structures and HIC retention times. The results showed that descriptors derived from 3D structures obtained after MD simulations were the most suitable for HIC retention time prediction with a R2 = 0.63 in an external test set. It was found that when using homology modelling, the resulting 3D structures became biased towards the used structural template. Performing an MD simulation therefore proved to be a necessary post-processing step for the mAb structures in order to relax the structures and allow them to attain a more natural conformation. Based on the results, the proposed workflow in this paper could therefore potentially contribute to aid in risk assessment of mAb candidates in early development.
Renin–Angiotensin System: An Important Player in the Pathogenesis of Acute Respiratory Distress Syndrome
Authors: Hrenak Jaroslav , Simko Fedor
Published: October 2020

Acute respiratory distress syndrome (ARDS) is characterized by massive inflammation, increased vascular permeability and pulmonary edema. Mortality due to ARDS remains very high and even in the case of survival, acute lung injury can lead to pulmonary fibrosis. The renin–angiotensin system (RAS) plays a significant role in these processes. The activities of RAS molecules are subject to dynamic changes in response to an injury. Initially, increased levels of angiotensin (Ang) II and des-Arg9-bradykinin (DABK), are necessary for an effective defense. Later, augmented angiotensin converting enzyme (ACE) 2 activity supposedly helps to attenuate inflammation. Appropriate ACE2 activity might be decisive in preventing immune-induced damage and ensuring tissue repair. ACE2 has been identified as a common target for different pathogens. Some Coronaviruses, including SARS-CoV-2, also use ACE2 to infiltrate the cells. A number of questions remain unresolved. The importance of ACE2 shedding, associated with the release of soluble ACE2 and ADAM17-mediated activation of tumor necrosis factor-α (TNF-α)-signaling is unclear. The roles of other non-classical RAS-associated molecules, e.g., alamandine, Ang A or Ang 1–9, also deserve attention. In addition, the impact of established RAS-inhibiting drugs on the pulmonary RAS is to be elucidated. The unfavorable prognosis of ARDS and the lack of effective treatment urge the search for novel therapeutic strategies. In the context of the ongoing SARS-CoV-2 pandemic and considering the involvement of humoral disbalance in the pathogenesis of ARDS, targeting the renin–angiotensin system and reducing the pathogen’s cell entry could be a promising therapeutic strategy in the struggle against COVID-19.