bluuebunny/crossref_metadata_2025 huggingface dataset:
Substandard and Semi-Dwarfing Citrus Rootstocks for More Intensive, Higher-Density, and Sustainable Plantation Systems
Authors: Hervalejo Aurea , Suárez María Paz , Arenas-Arenas Francisco José
Published: March 2021

An increasing number of intensive, dense, and sustainable citrus plantations have fostered a growing interest in addressing the future challenges of citrus crops: An increase in the world’s population, climate change, and globalization. Nutrient efficiency and the absence of vigorous citrus rootstocks are required for the success of these plantation systems. The agronomic performances of the ‘Lane Late’ orange cultivar on three substandard or semi-dwarfing citrus rootstocks (Forner-Alcaide no.5 (FA5), Forner-Alcaide no.13 (FA13), and Forner-Alcaide no.41 (FA41)) were evaluated in Spain in comparison with more traditional Mediterranean citrus rootstocks (Carrizo citrange (CA), Citrus macrophylla (MP), and ‘Cleopatra’ mandarin (CL)) under a poor mineral fertilization program over six growing seasons. FA13 and FA41 induced the smallest ‘Lane Late’ trees. Although the rootstock did not induce a significant effect on the ‘Lane Late’ yield efficiency (kg m−3), the highest values were recorded for ‘Lane Late’ on MP, CL and FA13. In this sense, FA13 showed a high productive potential (kg ha−1), given the possibility of narrowing the tree spacing (smaller tree size). Regarding the use of soil nutrients, FA13 was the most efficient citrus rootstock. Thus, FA13 stands out as the most suitable citrus rootstock for more intensive and sustainable plantation systems of the ‘Lane Late’ orange under Mediterranean conditions similar to those of this study.
Towards a Better and Harmonized Education in Antimicrobial Stewardship in European Veterinary Curricula
Authors: Espinosa-Gongora Carmen , Jessen Lisbeth , Dyar Oliver , Bousquet-Melou Alain , González-Zorn Bruno , Pulcini Céline , Re Giovanni , Schwarz Stefan , Timofte Dorina , Toutain Pierre-Louis , Guardabassi Luca ,   The PREPARE-VET Working Group,   ESCMID Study Group for Veterinary Microbiology (ESGVM),   ESCMID Study Group for Antimicrobial stewardshiP (ESGAP)
Published: March 2021

Education in antimicrobial stewardship (AMS) in veterinary medicine is essential to foster responsible antimicrobial use and control of antimicrobial resistance (AMR) in animals. AMS is listed by the EU and international organizations among the basic ‘Day One Competences’ required of veterinary students upon graduation. Our aim was to evaluate the quality of education of European veterinary students in AMS. We distributed a 27-item survey addressing the perceptions of preparedness and acquired skills on key topics related to AMS to final-year veterinary students in Europe. We collected 3423 complete answers from 89 veterinary schools in 30 countries. Selection of treatment strategies and awareness of emerging AMR problems were markedly different between countries. Overall, only one in four students was familiar with guidelines for antimicrobial use. The students perceived a medium-high impact of veterinary antimicrobial use on AMR in humans. Notably, 75% of the students felt the need for improved teaching on AMS, half of which also demanded more teaching on general antimicrobial therapy. Our results highlight several possible strategies to improve the quality of education, ranging from a better link between clinical rotations and the theory taught in pre-clinical modules, to a more effective introduction into best practices for antimicrobial use.
Building Information Modelling and Internet of Things Integration for Facility Management—Literature Review and Future Needs
Authors: Mannino Antonino , Dejaco Mario Claudio , Re Cecconi Fulvio
Published: March 2021

Digitisation of the built environment is seen as a significant factor for innovation in the Architecture, Engineering, Construction and Operation sector. However, lack of data and information in as-built digital models considerably limits the potential of Building Information Modelling in Facility Management. Therefore, optimisation of data collection and management is needed, all the more so now that Industry 4.0 has widened the use of sensors into buildings and infrastructures. A literature review on the two main pillars of digitalisation in construction, Building Information Modelling and Internet of Things, is presented, along with a bibliographic analysis of two citations and abstracts databases focusing on the operations stage. The bibliographic research has been carried out using Web of Science and Scopus databases. The article is aimed at providing a detailed analysis of BIM–IoT integration for Facility Management (FM) process improvements. Issues, opportunities and areas where further research efforts are required are outlined. Finally, four key areas of further research development in FM management have been proposed, focusing on optimising data collection and management.
Wavelength-Dependent Optical Nonlinear Absorption of Au-Ag Nanoparticles
Authors: Wang Jun , Shao Yabin , Chen Chunyu , Wu Wenzhi , Kong Degui , Gao Yachen
Published: March 2021

The nonlinear optical absorption properties of Au-Ag nanoparticles (NPs) were studied using an open-aperture Z-scan under a nanosecond pulsed laser with wavelengths of 450 nm, 510 nm, 550 nm, and 600 nm. The experimental results demonstrated that, when the laser intensity was 1.04 × 1013 W/m2, the Au-Ag NPs showed saturated absorption (SA). When the laser intensity was increased to 3.03 × 1013 W/m2, the switch from SA to reverse saturation absorption (RSA) occurred. The nonlinear absorption and its transformation were analyzed by using local surface plasmon resonance (LSPR) effect, bleaching of ground state plasmon, and free carrier absorption theory.
Concept, Design, Initial Tests and Prototype of Customized Upper Limb Prosthesis
Authors: Radu (Frenț) Corina , Roșu Maria Magdalena , Matei Lucian , Ungureanu Liviu Marian , Iliescu Mihaiela
Published: March 2021

This paper presents aspects of the concept and design of prostheses for the upper limb. The objective of this research is that of prototyping a customized prosthesis, with EMG signals that initiate the motion. The prosthesis’ fingers’ motions (as well as that of its hand and forearm parts) are driven by micro-motors, and assisted by the individualized command and control system. The software and hardware tandem concept of this mechatronic system enables complex motion (in the horizontal and vertical plane) with accurate trajectory and different set rules (gripping pressure, object temperature, acceleration towards the object). One important idea is regarding customization via reverse engineering techniques. Due to this, the dimensions and appearance (geometric characteristics) of the prosthesis would look like the human hand itself. The trajectories and motions of the fingers, thumbs, and joints have been studied by kinematic analysis with the matrix–vector method aided by Matlab. The concept and design of the mechanical parts allow for complex finger motion—rotational motion in two planes. The command and control system is embedded, and data received from the sensors are processed by a micro-controller for managing micro-motor control. Preliminary testing of the sensors and micro-motors on a small platform, Arduino, was performed. Prototyping of the mechanical components has been a challenge because of the high accuracy needed for the geometric precision of the parts. Several techniques of rapid prototyping were considered, but only DLP (digital light processing) proved to be the right one.
Shear Response of Members without Shear Reinforcement—Experiments and Analysis Using Shear Crack Propagation Theory (SCPT)
Authors: Schmidt Maximilian , Schmidt Philipp , Wanka Sebastian , Classen Martin
Published: March 2021

The determination of the ultimate shear capacity and the identification of the corresponding load-carrying mechanisms of concrete members without shear reinforcement has been an ongoing research topic for over 100 years. Based on a full mechanical model, the Shear Crack Propagation Theory (SCPT) enables to analyze and understand the ever-changing interplay of crack propagation, evolution of stresses at the crack tip and in uncracked concrete parts, as well as the activation of shear transfer actions within the growing shear crack during the entire loading process. In this paper, selected experimental investigations for further validation of the SCPT are presented. These beam shear test results are then compared to the theoretical results emerging from the SCPT algorithm. Finally, the evolution of different shear transfer actions (e.g., aggregate interlock and dowel action) during the entire loading process is evaluated and discussed.
Analysis of Torque and Force Induced by Rotary Nickel-Titanium Instruments during Root Canal Preparation: A Systematic Review
Authors: Thu Myint , Ebihara Arata , Adel Sherif , Okiji Takashi
Published: March 2021

The aim of this review was to provide a detailed literature analysis of torque and force generation during nickel-titanium rotary root canal instrumentation. We followed Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. An electronic search was performed using in PubMed and in journals for articles published in English from 1987 to June 2020 on studies that investigated dynamic torque and force in vivo or in vitro. We assessed article titles and abstracts to remove duplicates, and the titles and abstracts of the remaining articles were screened for eligibility. Full texts were read to verify eligibility by considering predetermined inclusion and exclusion criteria. Fifty-two out of 4096 studies met the inclusion criteria, from which we identified 26 factors that influence torque or force generation. Factors associated with higher torque or force generation and supported by multiple studies with mostly consistent results included convex triangle cross-sectional design, regressive taper, short pitch length, large instrument size, small canal size, single-length preparation technique, long preparation time, deep insertion depth, low rate of insertion, continuous rotation (torque), reciprocating motion (force), lower rotational speed and conventional alloy. However, several factors are interrelated, which obscured the independent effect of each factor, and there was insufficient scientific evidence supporting the influence of some factors.
Relationship between Ice Hockey-Specific Complex Test (IHCT) and Match Performance
Authors: Schwesig René , Laudner Kevin G. , Delank Karl-Stefan , Brill Richard , Schulze Stephan
Published: March 2021

This prospective study aimed to validate the IHCT using a match performance score (MPS). Seventeen 3rd league male professional ice hockey field players (mean ± SD; age: 28.0 ± 6.95 years; 10 forwards, 7 defenders) completed the IHCT and were observed in the subsequent competitive match period. Match performance data were collected over 2 months (number of matches: 13–15). Based on these evaluated matches, the MPS consisting of goals, assists, penalty time, plus-minus, and number of matches was calculated for each player. The IHCT contains load parameters (e.g., 10 m and 30 m skate times, transition/weave agility times with and without a puck, slap and wrist shot puck velocities on goal) and stress parameters (e.g., lactate, heart rate). The 30 m sprint skate without puck (r2 = 0.41) and the maximum wrist shot velocity before test (r2 = 0.36) were the test performance parameters with the highest relevance for match performance. The velocity of shots on goal (SOG) displayed the highest correlation for any on-ice performance parameter (30 m sprint without puck: r = 0.76). These results provide evidence of the validity of the IHCT and the usefulness of the MPS to quantify match performance. Obviously, sprint and shot velocities are important parameters to be a successful ice hockey player. Based on these findings, IHCT and MPS are valuable assessments for coaches to evaluate the individual match performance and the effectiveness of training programs. Further research is needed to extend the scope of the IHCT and MPS for 1st and 2nd league players and for a stronger differentiation between forwards and defenders.
Technological Advances in Ozone and Ozonized Water Spray Disinfection Devices
Authors: Mascarenhas Luis Alberto Breda , Oliveira Fabricia Oliveira , da Silva Eduardo Santos , dos Santos Laerte Marlon Conceição , de Alencar Pereira Rodrigues Leticia , Neves Paulo Roberto Freitas , Santos Alex Álisson Bandeira , Moreira Greta Almeida Fernandes , Lobato Gabriela Monteiro , Nascimento Carlos , Gerhardt Marcelo , Machado Bruna Aparecida Souza
Published: March 2021

To control infectious diseases such as the severe acute respiratory syndrome coronavirus (Covid-19) that caused the current pandemic, disinfection measures are essential. Among building measures, disinfection chambers can help to decrease the transmission rate through the sanitizing capacity of the disinfectant used, which can thereby clean surfaces or humans. Out of existing biocides, ozone is considered one of the safest for humans, but one of the most powerful oxidizers, making the substance a better alternative as the biocidal solution in disinfection chambers. Analyses were carried out by using all patented documents related to disinfection chambers that used ozone as a disinfectant. A Derwent Innovation Index (DII) database search was undertaken to find these patents. Patent prospecting resulted in 620 patent documents that were divided into 134 patent families. There was no technology related to protective barriers for individuals, and the majority of patents in the retrieved data aimed at sterilizing medical devices and surfaces. Given that the specific Cooperative Patent Classification (CPC) code for ozone dissolved in liquid was used in the methodology search, but not included among the 10 most used codes in the patents, the use of ozonized water may be an innovative approach in the technology landscape of sterilization chambers.
Lipid Peroxidation in Subretinal Fluid: Some Light on the Prognosis Factors
Authors: Bosch-Morell Francisco , García-Gen Enrique , Mérida Salvador , Penadés Mariola , Desco Carmen , Navea Amparo
Published: March 2021

The aim of this study was to identify a relation between the clinical characteristics and differences in lipid peroxidation in the subretinal fluid (SRF) of rhegmatogenous retinal detached patients by malondialdehyde (MDA) quantification. We collected 65 SRF samples from consecutive patients during scleral buckling surgery in rhegmatogenous retinal detachment (RRD) eyes. In addition to a complete ophthalmic evaluation, we studied the refractive status, evolution time, and the number of detached retinal quadrants to establish the extension of RRD. We studied the clinical aspects and oxidative stress and compared the characteristics among groups. We found that neither the evolution time of RRD nor the patients’ age correlated with the MDA concentration in the SRF. The MDA and the protein content of the SRF increased in the patients with high myopia and with more extended RRD. Our results suggest that oxidative imbalance was important in more extended retinal detachment (RD) and in myopic eyes and should be taken into account in the managing of these cases.
Fetal High-Density Lipoproteins: Current Knowledge on Particle Metabolism, Composition and Function in Health and Disease
Authors: Stadler Julia T. , Wadsack Christian , Marsche Gunther
Published: March 2021

Cholesterol and other lipids carried by lipoproteins play an indispensable role in fetal development. Recent evidence suggests that maternally derived high-density lipoprotein (HDL) differs from fetal HDL with respect to its proteome, size, and function. Compared to the HDL of adults, fetal HDL is the major carrier of cholesterol and has a unique composition that implies other physiological functions. Fetal HDL is enriched in apolipoprotein E, which binds with high affinity to the low-density lipoprotein receptor. Thus, it appears that a primary function of fetal HDL is the transport of cholesterol to tissues as is accomplished by low-density lipoproteins in adults. The fetal HDL-associated bioactive sphingolipid sphingosine-1-phosphate shows strong vasoprotective effects at the fetoplacental vasculature. Moreover, lipoprotein-associated phospholipase A2 carried by fetal-HDL exerts anti-oxidative and athero-protective functions on the fetoplacental endothelium. Notably, the mass and activity of HDL-associated paraoxonase 1 are about 5-fold lower in the fetus, accompanied by an attenuation of anti-oxidative activity of fetal HDL. Cholesteryl ester transfer protein activity is reduced in fetal circulation despite similar amounts of the enzyme in maternal and fetal serum. This review summarizes the current knowledge on fetal HDL as a potential vasoprotective lipoprotein during fetal development. We also provide an overview of whether and how the protective functionalities of HDL are impaired in pregnancy-related syndromes such as pre-eclampsia or gestational diabetes mellitus.
Potential Impact of Human Cytomegalovirus Infection on Immunity to Ovarian Tumours and Cancer Progression
Authors: Cox Momodou , Kartikasari Apriliana E. R. , Gorry Paul R. , Flanagan Katie L. , Plebanski Magdalena
Published: March 2021

Ovarian cancer (OC) is one of the most common, and life-threatening gynaecological cancer affecting females. Almost 75% of all OC cases are diagnosed at late stages, where the 5-year survival rate is less than 30%. The aetiology of the disease is still unclear, and there are currently no screening method nor effective treatment strategies for the advanced disease. A growing body of evidence shows that human cytomegalovirus (HCMV) infecting more than 50% of the world population, may play a role in inducing carcinogenesis through its immunomodulatory activities. In healthy subjects, the primary HCMV infection is essentially asymptomatic. The virus then establishes a life-long chronic latency primarily in the hematopoietic progenitor cells in the bone marrow, with periodic reactivation from latency that is often characterized by high levels of circulating pro-inflammatory cytokines. Currently, infection-induced chronic inflammation is considered as an essential process for OC progression and metastasis. In line with this observation, few recent studies have identified high expressions of HCMV proteins on OC tissue biopsies that were associated with poor survival outcomes. Active HCMV infection in the OC tumour microenvironment may thus directly contribute to OC progression. In this review, we highlight the potential impact of HCMV infection-induced immunomodulatory effects on host immune responses to OC that may promote OC progression.
Dulaglutide Alone and in Combination with Empagliflozin Attenuate Inflammatory Pathways and Microbiome Dysbiosis in a Non-Diabetic Mouse Model of NASH
Authors: Hupa-Breier Katharina Luise , Dywicki Janine , Hartleben Björn , Wellhöner Freya , Heidrich Benjamin , Taubert Richard , Mederacke Young-Seon Elisabeth , Lieber Maren , Iordanidis Konstantinos , Manns Michael P. , Wedemeyer Heiner , Hardtke-Wolenski Matthias , Jaeckel Elmar
Published: March 2021

Dysregulation of glucose homeostasis plays a major role in the pathogenesis of non-alcoholic steatohepatitis (NASH) as it activates proinflammatory and profibrotic processes. Beneficial effects of antiglycemic treatments such as GLP-1 agonist or SGLT-2 inhibitor on NASH in patients with diabetes have already been investigated. However, their effect on NASH in a non-diabetic setting remains unclear. With this aim, we investigated the effect of long-acting GLP1-agonist dulaglutide and SGLT-2 inhibitor empagliflozin and their combination in a non-diabetic mouse model of NASH. C57BL/6 mice received a high-fat-high-fructose (HFHC) diet with a surplus of cholesterol for 16 weeks. After 12 weeks of diet, mice were treated with either dulaglutide, empagliflozin or their combination. Dulaglutide alone and in combination with empagliflozin led to significant weight loss, improved glucose homeostasis and diminished anti-inflammatory and anti-fibrotic pathways. Combination of dulaglutide and empagliflozin further decreased MoMFLy6CHigh and CD4+Foxp3+ T cells. No beneficial effects for treatment with empagliflozin alone could be shown. While no effect of dulaglutide or its combination with empaglifozin on hepatic steatosis was evident, these data demonstrate distinct anti-inflammatory effects of dulaglutide and their combination with empagliflozin in a non-diabetic background, which could have important implications for further treatment of NASH.
Typical Pediatric Brain Tumors Occurring in Adults—Differences in Management and Outcome
Authors: Greuter Ladina , Guzman Raphael , Soleman Jehuda
Published: March 2021

Adult brain tumors mostly distinguish themselves from their pediatric counterparts. However, some typical pediatric brain tumors also occur in adults. The aim of this review is to describe the differences between classification, treatment, and outcome of medulloblastoma, pilocytic astrocytoma, and craniopharyngioma in adults and children. Medulloblastoma is a WHO IV posterior fossa tumor, divided into four different molecular subgroups, namely sonic hedgehog (SHH), wingless (WNT), Group 3, and Group 4. They show a different age-specific distribution, creating specific outcome patterns, with a 5-year overall survival of 25–83% in adults and 50–90% in children. Pilocytic astrocytoma, a WHO I tumor, mostly found in the supratentorial brain in adults, occurs in the cerebellum in children. Complete resection improves prognosis, and 5-year overall survival is around 85% in adults and >90% in children. Craniopharyngioma typically occurs in the sellar compartment leading to endocrine or visual field deficits by invasion of the surrounding structures. Treatment aims for a gross total resection in adults, while in children, preservation of the hypothalamus is of paramount importance to ensure endocrine development during puberty. Five-year overall survival is approximately 90%. Most treatment regimens for these tumors stem from pediatric trials and are translated to adults. Treatment is warranted in an interdisciplinary setting specialized in pediatric and adult brain tumors.
Dietary Fats, Serum Cholesterol and Liver Cancer Risk: A Systematic Review and Meta-Analysis of Prospective Studies
Authors: Zhao Longgang , Deng Chuanjie , Lin Zijin , Giovannucci Edward , Zhang Xuehong
Published: March 2021

To quantify the associations between dietary fats and their major components, as well as serum levels of cholesterol, and liver cancer risk, we performed a systematic review and meta-analysis of prospective studies. We searched PubMed, Embase, and Web of Science up to October 2020 for prospective studies that reported the risk estimates of dietary fats and serum cholesterol for liver cancer risk. We carried out highest versus lowest intake or level and dose-response analyses. Higher intake of dietary saturated fatty acids (SFA) was associated with a higher liver cancer risk in both category analysis (relative risk [RR]highest vs. lowest intake = 1.34, 95% confidence interval [CI]: 1.06, 1.69) and dose-response analysis (RR1% energy = 1.04, 95%CI: 1.01, 1.07). Higher serum total cholesterol was inversely associated with liver cancer but with large between-studies variability (RR1 mmol/L = 0.72, 95%CI: 0.69, 0.75, I2 = 75.3%). The inverse association was more pronounced for serum high-density lipoprotein (HDL) cholesterol (RR1 mmol/L = 0.42, 95%CI: 0.27, 0.64). Higher intake of dietary SFA was associated with higher risk of liver cancer while higher serum levels of cholesterol and HDL were associated with a lower risk of liver cancer with high between-studies variability.
Effects of Surface Oxygen-Containing Groups of the Flowerlike Carbon Nanosheets on Palladium Dispersion, Catalytic Activity and Stability in Hydrogenolytic Debenzylation of Tetraacetyldibenzylhexaazaisowurtzitane
Authors: Chen Yun , Ding Xinlei , Qiu Wenge , Song Jianwei , Nan Junping , Bai Guangmei , Pang Siping
Published: March 2021

The influence of the surface chemical properties of the carbon support on the Pd dispersion, activity and stability of Pd(OH)2/C catalyst for the hydrogenolytic debenzylation of tetraacetyldibenzylhexaazaisowurtzitane (TADB) was studied in detail. The flowerlike nanosheet carbon material (NSC) was chosen as the pristine support, meanwhile chemical oxidation with nitric acid and physical calcination at 600 °C treatments were used to modify its surface properties, which were denoted as NSCox-2 (treated with 20 wt% HNO3) and NSC-600, respectively. The three carbon supports and the corresponding catalysts of Pd/NSC, Pd/NSC-600, and Pd/NSCox-2 were characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), nitrogen sorption isotherm measurement (BET), powder X-ray diffraction (XRD), Raman spectra, X-ray photoelectron spectra (XPS), temperature-programmed desorption (TPD), temperature-programmed reduction (H2-TPR), thermogravimetric analysis (TG), and element analysis. The debenzylation activities of Pd/NSC, Pd/NSC-600, and Pd/NSCox-2, as well as the three catalysts after pre-reduction treatment were also evaluated. It was found that the activity and stability of the Pd(OH)2/C catalysts in the debenzylation reaction highly depended on the content of surface oxygen-containing groups of the carbon support.
CO2 Methanation Using Multimodal Ni/SiO2 Catalysts: Effect of Support Modification by MgO, CeO2, and La2O3
Authors: Mihet Maria , Dan Monica , Barbu-Tudoran Lucian , Lazar Mihaela D.
Published: March 2021

Ni/oxide-SiO2 (oxide: MgO, CeO2, La2O3, 10 wt.% target concentration) catalyst samples were prepared by successive impregnation of silica matrix, first with supplementary oxide, and then with Ni (10 wt.% target concentration). The silica matrix with multimodal pore structure was prepared by solvothermal method. The catalyst samples were structurally characterized by N2 adsorption-desorption, XRD, SEM/TEM, and functionally evaluated by temperature programmed reduction (TPR), and temperature programmed desorption of hydrogen (H2-TPD), or carbon dioxide (CO2-TPD). The addition of MgO and La2O3 leads to a better dispersion of Ni on the catalytic surface. Ni/LaSi and Ni/CeSi present a higher proportion of moderate strength basic sites for CO2 activation compared to Ni/Si, while Ni/MgSi lower. CO2 methanation was performed in the temperature range of 150–350 °C and at atmospheric pressure, all silica supported Ni catalysts showing good CO2 conversion and CH4 selectivity. The best catalytic activity was obtained for Ni/LaSi: CO2 conversion of 83% and methane selectivity of 98%, at temperatures as low as 250 °C. The used catalysts preserved the multimodal pore structure with approximately the same pore size for the low and medium mesopores. Except for Ni/CeSi, no particle sintering occurs, and no carbon deposition was observed for any of the tested catalysts.
Nuclear Translocation of SRPKs Is Associated with 5-FU and Cisplatin Sensitivity in HeLa and T24 Cells
Authors: Sigala Ioanna , Koutroumani Maria , Koukiali Anastasia , Giannakouros Thomas , Nikolakaki Eleni
Published: March 2021

Serine/arginine protein kinases (SRPKs) phosphorylate Arg/Ser dipeptide-containing proteins that play crucial roles in a broad spectrum of basic cellular processes. The existence of a large internal spacer sequence that separates the bipartite kinase catalytic core and anchors the kinases in the cytoplasm is a unique structural feature of SRPKs. Here, we report that exposure of HeLa and T24 cells to DNA damage inducers triggers the nuclear translocation of SRPK1 and SRPK2. Furthermore, we show that nuclear SRPKs did not protect from, but on the contrary, mediated the cytotoxic effects of genotoxic agents, such as 5-fluorouracil (5-FU) and cisplatin. Confirming previous data showing that the kinase activity is essential for the entry of SRPKs into the nucleus, SRPIN340, a selective SRPK1/2 inhibitor, blocked the nuclear accumulation of the kinases, thus diminishing the cytotoxic effects of the drugs. ATR/ATM-dependent phosphorylation of threonine 326 and serine 408 in the spacer domain of SRPK1 was essential for the redistribution of the kinase to the nucleus. Substitution of either of these two residues to alanine or inhibition of ATR/ATM kinase activity abolished nuclear localization of SRPK1 and conferred tolerance to 5-FU treatment. These findings suggest that SRPKs may play an important role in linking cellular signaling to DNA damage in eukaryotic cells.
Benign Convulsions with Mild Rotavirus and Norovirus Gastroenteritis: Nationwide Data from the Health Insurance Review and Assessment Service in South Korea
Authors: Kim Dong Hyun , Ha Dong Jun , Lee Yeong Seok , Chun Min Jun , Kwon Young Se
Published: March 2021

There have been no large-scale studies on the epidemiology of benign convulsions with mild gastroenteritis (CwG) since the introduction of the rotavirus vaccine in South Korea in 2007. This study aimed to analyze the trends in rotavirus gastroenteritis (RVGE) and rotavirus-associated CwG (RaCwG) after rotavirus vaccination. Further, we aimed to analyze changes in norovirus gastroenteritis (NVGE) and norovirus-associated CwG (NaCwG) using nationwide data from the Korean Health Insurance Review and Assessment Service. Between 2007 and 2019, this study analyzed children aged <6 years who were diagnosed with RVGE, NVGE, RaCwG and NaCwG. The changes in the prevalence of each disease and the ratio of CwG to enteritis were analyzed and the effects of age, sex and season were also analyzed. RVGE, RaCwG, NVGE and NaCwG were diagnosed in 273,898, 4246, 35,593 and 337 patients, respectively. The prevalence of RVGE was on a decreasing trend every year, but the prevalence of NaCwG and NVGE was on an increasing trend. There was a significant annual increase in the ratio of CwG to enteritis in both viruses. In order to control the prevalence of RaCwG, measures other than the rotavirus vaccine are required and measures to prevent norovirus are necessary.
Children Involved in Team Sports Show Superior Executive Function Compared to Their Peers Involved in Self-Paced Sports
Authors: De Waelle Silke , Laureys Felien , Lenoir Matthieu , Bennett Simon J. , Deconinck Frederik J.A.
Published: March 2021

Children’s motor and cognitive functions develop rapidly during childhood. Physical activity and executive function are intricately linked during this important developmental period, with physical activity interventions consistently proving to benefit children’s executive function. However, it is less clear which type of physical activity shows the strongest associations with executive function in children. Therefore, this study compared executive function performance of children aged 8 to 12 that either participated in team sports or self-paced sports or were not involved in any kind of organized sports (non-athletes). Results demonstrate that children participating in team sports show superior executive function compared to children participating in self-paced sports and non-athletes. Importantly, children participating in self-paced sports do not outperform non-athletes when it comes to executive function. This study is the first to show that even at a very young age, team sports athletes outperform athletes from self-paced sports as well as non-athletes on a multifaceted and comprehensive test battery for executive function. Furthermore, our findings support the hypothesis that cognitively engaging physical activity, such as participation in team sports, might show stronger associations with executive functioning compared to other types of sports and physical activity.
Development of a Stress Scale for Siblings of Childhood Cancer Patients
Authors: Yu Juyoun , Bang Kyung-Sook , Kang Hyoung
Published: March 2021

Most siblings of childhood cancer patients (SCCP) report not only post-traumatic stress but also daily stresses due to changes in their daily lives. The purpose of this study was to develop a stress scale for SCCP and to examine the validity and reliability of the scale. Based on conceptual analysis, 40 preliminary items were selected. After its content validity was determined by six experts, 37 items were chosen. For the psychometric testing, 125 SCCPs, aged 11–16, were surveyed. Through item analysis and exploratory factor analysis for construct validity, 27 items explained 61.2% of the variance, and they were categorized into six factors. Criterion validity was confirmed by examining the overall correlation with standard instruments according to the age group. Reliability was evaluated using Cronbach’s alpha (0.91) and test-retest correlation (r = 0.597). This self-administered questionnaire with a 4-point Likert-type scale may be useful in clarifying and measuring stress levels in SCCPs.
Synergistic Effects of Dexamethasone and Dexmedetomidine in Extending the Effects of Pectoral I and Pectoral II Blocks for Postoperative Analgesia Following Total Mastectomy with Lymph Node Dissection
Authors: Chitneni Ahish , Hasoon Jamal , Urits Ivan , Viswanath Omar , Kaye Alan D. , Eskander Jonathan
Published: March 2021

Regional anesthesia is an important aspect of the overall anesthetic plan for patients. It has the potential to offer superior benefits compared to opioid-based treatment and is an important component of Enhanced Recovery after Surgery (ERAS) protocols. The use of the pectoral type I (PECS I) and pectoral type II blocks (PECS II) has been shown to reduce postoperative pain and opioid consumption in patients undergoing complete mastectomy and breast surgery. We describe the use of dexamethasone and dexmedetomidine to prolong the analgesic effects of these regional blocks in a patient undergoing total mastectomy with lymph node dissection.
Numerical Simulation of Ammonothermal Crystal Growth of GaN—Current State, Challenges, and Prospects
Authors: Schimmel Saskia , Tomida Daisuke , Ishiguro Tohru , Honda Yoshio , Chichibu Shigefusa , Amano Hiroshi
Published: March 2021

Numerical simulations are a valuable tool for the design and optimization of crystal growth processes because experimental investigations are expensive and access to internal parameters is limited. These technical limitations are particularly large for ammonothermal growth of bulk GaN, an important semiconductor material. This review presents an overview of the literature on simulations targeting ammonothermal growth of GaN. Approaches for validation are also reviewed, and an overview of available methods and data is given. Fluid flow is likely in the transitional range between laminar and turbulent; however, the time-averaged flow patterns likely tend to be stable. Thermal boundary conditions both in experimental and numerical research deserve more detailed evaluation, especially when designing numerical or physical models of the ammonothermal growth system. A key source of uncertainty for calculations is fluid properties under the specific conditions. This originates from their importance not only in numerical simulations but also in designing similar physical model systems and in guiding the selection of the flow model. Due to the various sources of uncertainty, a closer integration of numerical modeling, physical modeling, and the use of measurements under ammonothermal process conditions appear to be necessary for developing numerical models of defined accuracy.
Combinatorial Materials Design Approach to Investigate Adhesion Layer Chemistry for Optimal Interfacial Adhesion Strength
Authors: Schoeppner Rachel L. , Putz Barbara , Taylor Aidan A. , Pethö Laszlo , Thomas Keith , Antonin Olivier , Nelis Thomas , Michler Johann
Published: March 2021

A combinatorial material adhesion study was used to optimize the composition of an adhesion promoting layer for a nanocrystalline diamond (NCD) coating on silicon. Three different adhesion promoting metals, namely W, Cr, and Ta, were selected to fabricate arrays of co-sputtered binary alloy films, with patches of seven different, distinct alloy compositions for each combination, and single element reference films on a single Si wafer (three wafers in total; W–Cr, Cr–Ta, Ta–W). Scratch testing was used to determine the critical failure load and practical work of adhesion for the NCD coatings as a function of adhesion layer chemistry. All tested samples eventually exhibit delamination of the NCD coating, with buckles radiating perpendicularly away from the scratch track. Application of any of the presented adhesion layers yields an increase of the critical failure load for delamination as compared to NCD on Si. While the influence of adhesion layers on the maximum buckle length is less pronounced, shorter buckles are obtained with pure W and Cr–Ta alloy layers. As a general rule, the addition of an adhesion layer showed a 75% improvement in the measured adhesion energies of the NCD films compared to the NCD coating without an adhesion layer, with specific alloys and compositions showing up to 125% increase in calculated practical work of adhesion.
Biochar-Supported BiOx for Effective Electrosynthesis of Formic Acid from Carbon Dioxide Reduction
Authors: Zeng Juqin , Jagdale Pravin , Lourenço Mirtha A. O. , Farkhondehfal M. Amin , Sassone Daniele , Bartoli Mattia , Pirri Candido Fabrizio
Published: March 2021

The electrochemical reduction of carbon dioxide (CO2) to value-added chemicals and fuels has attracted worldwide interest for its potential to address various contemporary global issues such as CO2-related climate change, the earth’s carbon deficit and the energy crisis. In the development of this technology, many efforts have been focused on the design of inexpensive, eco-friendly and effective catalysts. In this work, a bismuth (Bi)-based material was simply synthesized via a scalable method and fully characterized by physical, chemical and electrochemical techniques. The catalyst material consisted of Bi/Bi2O3 nanoparticles and a biochar prevenient from the pyrolysis of brewed coffee waste. It was observed that the surface of the biochar was thoroughly decorated with nanoparticles. Due to its uniform surface, the biochar–BiOx electrode demonstrated good selectivity for CO2 reduction, showing a faradaic efficiency of more than 90% for CO and HCOOH formation in a wide potential range. Particularly, the selectivity for HCOOH reached more than 80% from −0.9 V to −1.3 V vs the reversible hydrogen electrode and peaks at 87%. Besides the selectivity, the production rate of HCOOH also achieved significant values with a maximum of 59.6 mg cm−2 h−1, implying a good application potential for biochar–BiOx material in the conversion of CO2 to HCOOH.
Neuropathic Pain in the Elderly
Authors: Giovannini Silvia , Coraci Daniele , Brau Fabrizio , Galluzzo Vincenzo , Loreti Claudia , Caliandro Pietro , Padua Luca , Maccauro Giulio , Biscotti Lorenzo , Bernabei Roberto
Published: March 2021

Neuropathic pain due to a lesion or a disease of the somatosensory system often affects older people presenting several comorbidities. Moreover, elderly patients are often poly-medicated, hospitalized and treated in a nursing home with a growing risk of drug interaction and recurrent hospitalization. Neuropathic pain in the elderly has to be managed by a multidimensional approach that involves several medical, social and psychological professionals in order to improve the quality of life of the patients and, where present, their relatives.
Multicentre Evaluation of Hepika Test Clinical Accuracy in Diagnosing HPV-Induced Cancer and Precancerous Lesions of the Uterine Cervix
Authors: Gustinucci Daniela , Ciccocioppo Lucia , Coppola Luigi , Negri Giovanni , Zannoni Gianfranco , Passamonti Basilio , Cesarini Elena , Ianzano Ciro , Andreano Tiziana , Pireddu Anjuta , Giorgi-Rossi Paolo
Published: March 2021

Objective: To evaluate the clinical accuracy of Hepika test to identify cancer/precancerous lesions of the uterine cervix. Materials and Methods: A multicentre retrospective study was carried out in 2018 and included 330 liquid-based cytology samples from three Italian centres of women aged 25–64 who had been tested for the human papillomavirus (HPV) and whose histology or follow-up outcome was known. Hepika is an enzyme-linked immunosorbent assay (ELISA) targeting the protein complexes E6#p53 and E7#pRb. After excluding samples without sufficient residual material, the clinical accuracy of Hepika test was evaluated in 274 samples: adenocarcinoma (ADC) (4), squamous cell carcinoma (SCC) (7), adenocarcinoma in situ (AIS) (1), cervical intraepithelial neoplasia (CIN) grade 3 (60), CIN2 (51), CIN1 (34), and negative histology (117). Association, sensitivity, and specificity for carcinoma, CIN3+ and CIN2+ are reported. Results: Positive Hepika test was associated with a high probability of carcinoma (odds ratio (DOR) = 33.68, 95% confidence interval (CI) 7.0–163.1); sensitivity was 81.8%, specificity, 88.2%. A positive Hepika test showed a weaker association with CIN3+ lesions (DOR = 3.5; 95% CI 1.75–6.99) and lower sensitivity (27.8%). Conclusion: The Hepika test was found to be an accurate biomarker for HPV-induced cervical carcinoma. Population-based prospective studies are needed to confirm the clinical usefulness of the Hepika test in the differential diagnosis of HPV-induced invasive lesions.
CD5L as an Extracellular Vesicle-Derived Biomarker for Liquid Biopsy of Lung Cancer
Authors: Choi Eun-Sook , Faruque Hasan Al , Kim Jung-Hee , Kim Kook Jin , Choi Jin Eun , Kim Bo A. , Kim Bora , Kim Ye Jin , Woo Min Hee , Park Jae Yong , Hur Keun , Lee Mi-Young , Kim Dong Su , Lee Shin Yup , Kim Eunjoo
Published: March 2021

Cancer screening and diagnosis can be achieved by analyzing specific molecules within serum-derived extracellular vesicles (EVs). This study sought to profile EV-derived proteins to identify potential lung cancer biomarkers. EVs were isolated from 80 serum samples from healthy individuals and cancer patients via polyethylene glycol (PEG)-based precipitation and immunoaffinity separation using antibodies against CD9, CD63, CD81, and EpCAM. Proteomic analysis was performed using 2-D gel electrophoresis and matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI–TOF MS). The expression of proteins that were differentially upregulated in the EVs or tissue of lung cancer samples was validated by Western blotting. The area under the curve (AUC) was calculated to assess the predictability of each differentially expressed protein (DEP) for lung cancer. A total of 55 upregulated protein spots were selected, seven of which (CD5L, CLEC3B, ITIH4, SERFINF1, SAA4, SERFINC1, and C20ORF3) were found to be expressed at high levels in patient-derived EVs by Western blotting. Meanwhile, only the expression of EV CD5L correlated with that in cancer tissues. CD5L also demonstrated the highest AUC value (0.943) and was found to be the core regulator in a pathway related to cell dysfunction. Cumulatively, these results show that EV-derived CD5L may represent a potential biomarker—detected via a liquid biopsy—for the noninvasive diagnosis of lung cancer.
Impact of Chronic Prostatitis on the PI-RADS Score 3: Proposal for the Addition of a Novel Binary Suffix
Authors: Merat Sascha , Blümlein Theresa , Klarhöfer Markus , Nickel Dominik , Singer Gad , Zöllner Frank G. , Schoenberg Stefan O. , Kubik-Huch Rahel A. , Hausmann Daniel , Hefermehl Lukas
Published: March 2021

We examined the impact of chronic prostatitis on the diagnostic performance of multiparametric magnetic resonance imaging (mpMRI). In this retrospective study, 63 men underwent 3T mpMRI followed by MRI/ultrasound fusion biopsy to exclude/confirm clinically significant prostate cancer (csPCa). A total of 93 lesions were included for evaluation. Images were assessed by two radiologists. Prostatitis was graded visually on T2-weighted and contrast-enhanced sequences. The correlation of prostatitis features with the assigned Prostate Imaging Reporting and Data System (PI-RADS) and the presence of csPCa were assessed, and the clinical and functional imaging parameters for differentiating between prostatitis and significant tumors were examined. Histopathological analysis was used as the reference standard. The rate of PI-RADS 3 scores tended to be higher in the presence of radiologically severe prostatitis compared with no/discrete prostatitis (n = 52 vs. n = 9; p = 0.225). In severe prostatitis, csPCa was determined in only 7.7% (4/52) of PI-RADS 3 lesions. In severe chronic prostatitis, a binary prostatitis suffix (e.g., PI-RADS 3 i+ versus i−) within the radiological report may help assess the limitations of mpMRI interpretability because of severe prostatitis and avoid unnecessary biopsies. Mean apparent diffusion coefficient (ADCmean) was the best marker (cutoff 0.93 × 10−3 mm2/s) to differentiate between csPCa/non csPCa in severe prostatitis.
Physical Limitations on Fundamental Efficiency of SET-Based Brownian Circuits
Authors: Ercan İlke , Sütgöl Zeynep Duygu , Özhan Faik Ozan
Published: March 2021

Brownian circuits are based on a novel computing approach that exploits quantum fluctuations to increase the efficiency of information processing in nanoelectronic paradigms. This emerging architecture is based on Brownian cellular automata, where signals propagate randomly, driven by local transition rules, and can be made to be computationally universal. The design aims to efficiently and reliably perform primitive logic operations in the presence of noise and fluctuations; therefore, a Single Electron Transistor (SET) device is proposed to be the most appropriate technology-base to realize these circuits, as it supports the representation of signals that are token-based and subject to fluctuations due to the underlying tunneling mechanism of electric charge. In this paper, we study the physical limitations on the energy efficiency of the Single-Electron Transistor (SET)-based Brownian circuit elements proposed by Peper et al. using SIMON 2.0 simulations. We also present a novel two-bit sort circuit designed using Brownian circuit primitives, and illustrate how circuit parameters and temperature affect the fundamental energy-efficiency limitations of SET-based realizations. The fundamental lower bounds are obtained using a physical-information-theoretic approach under idealized conditions and are compared against SIMON 2.0 simulations. Our results illustrate the advantages of Brownian circuits and the physical limitations imposed on their SET-realizations.
Differential Replication for Credit Scoring in Regulated Environments
Authors: Unceta Irene , Nin Jordi , Pujol Oriol
Published: March 2021

Differential replication is a method to adapt existing machine learning solutions to the demands of highly regulated environments by reusing knowledge from one generation to the next. Copying is a technique that allows differential replication by projecting a given classifier onto a new hypothesis space, in circumstances where access to both the original solution and its training data is limited. The resulting model replicates the original decision behavior while displaying new features and characteristics. In this paper, we apply this approach to a use case in the context of credit scoring. We use a private residential mortgage default dataset. We show that differential replication through copying can be exploited to adapt a given solution to the changing demands of a constrained environment such as that of the financial market. In particular, we show how copying can be used to replicate the decision behavior not only of a model, but also of a full pipeline. As a result, we can ensure the decomposability of the attributes used to provide explanations for credit scoring models and reduce the time-to-market delivery of these solutions.