bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Ripple Control on Induction Motors
Authors: Gnaciński Piotr , Pepliński Marcin , Muc Adam , Hallmann Damian , Jankowski Piotr
Published: November 2023

One method for the remote management of electrical equipment is ripple control (RC), based on the injection of voltage interharmonics into the power network to transmit information. The disadvantage of this method is its negative impact on energy consumers, such as light sources, speakers, and devices counting zero crossings. This study investigates the effect of RC on low-voltage induction motors through the use of experimental and finite element methods. The results show that the provisions concerning RC included in the European Standard EN 50160 Voltage Characteristics of Electricity Supplied by Public Distribution Network are imprecise, failing to protect induction motors against excessive vibration.
Managing the Expectations of Doctoral Students and Their Supervisors: A UK Perspective
Authors: Palmer Clive , Sprake Andrew , Hughes Chris
Published: November 2023

The management of expectations in doctoral education relates to the negotiation and agreement of a learning contract denoting actions and initiatives between a student and a supervisor. A learning contract is a set of understandings of what things, actions and initiatives might reasonably be expected from whom, in the course of learning, where there is a natural power imbalance. This is important so that both scholarly and material progress can be made along all points of the doctoral learning experience, i.e., that learning is personalised, professional and productive towards an original contribution of knowledge. It is the evidencing of this continual learning process through research that is deemed to be doctoral at the final examination stage. A doctoral student is a learner on the highest degree pathway that is available at all UK universities. This typically results in a thesis, marking the end point of being supervised whereupon an assessment or examination takes place, which, in UK universities, is called a viva voce (Latin: the living voice). This is a verbal account or defence of the thesis document by the student, made to two or three examiners who comprise the examination team. In the UK, the viva examination is a private event, while elsewhere, for example, across Europe and North America, the examination can be a public event. A student on a doctoral programme usually has a period of registration that is 3 years full-time or 6 years part-time. Other terms that can be used interchangeably around doctoral supervision are candidate (for the student) and candidature, which is their period of registration. Supervisors also have roles denoted as the Director of Studies (DoS) or Principal Investigator (PI). The supervision team is led by a Director of Studies (or PI) who is often the most experienced scholar who teaches, guides and mentors their student’s learning through the research they conduct. There are usually at least two supervisors in a supervision team in the UK, but there can be more as required depending upon the specialisms and topics being researched. Expectations formed by either the student or the supervisor(s) can be about physical resources to embark upon a passage of learning through a doctoral programme, or more typically, the discussion of expectations relates to managing the behaviours of students and supervisors in their respective roles. Managed expectations help to achieve a balance between the intellectual sharing of expertise by the supervisor with the self-directed initiatives for learning, which are taken by the student. The aim of managing expectations is to help a student move from dependence in their learning at the start of their programme to becoming an independent doctoral-level scholar who, once graduated as doctor, can act autonomously to conduct their own research, or even embark upon supervising others’ research in the future.
Period Poverty from a Public Health and Legislative Perspective
Authors: Mann Simranjot , Byrne Sharon K.
Published: November 2023

Period poverty is a global issue that needs to be addressed as a public health crisis. It is directly related to Sustainable Health Goals three, four, five, six, and eight. Period poverty adversely affects the health of anyone capable of menstruating, which is nearly half of the world population, at the physiological, emotional, and psychosocial level. Biases, cultural beliefs, ethical reproductive justice issues, social stigma, and systemic factors contribute to period poverty. Every month, certain menstruators are disproportionately impacted by period poverty and struggle to access basic hygienic necessities. Important stakeholders include not only the individual who experiences menses but also educators and school systems, healthcare professionals, policymakers, public health officials, and researchers. Everyone has a role in addressing period poverty by voting for officials that proactively support legislation, policy, and programs at all levels to effectively advocate for menstrual equity and address barriers contributing to period poverty. This includes policies that increase access to menstrual hygiene products, safe menstrual management methods, and reproductive and women’s health education. Programs globally that focus on capacity building and sustainability strategies can be used as models to reduce period poverty, thereby fostering a sense of empowerment and menstruators’ sense of autonomy, dignity, and equality.
Potential Roles of microRNAs for Assessing Cardiovascular Risk in Pre-Eclampsia-Exposed Postpartum Women and Offspring
Authors: Mohd Isa Nurul Iffah , Syafruddin Saiful Effendi , Mokhtar Mohd Helmy , Zainal Abidin Shahidee , Jaffar Farah Hanan Fathihah , Ugusman Azizah , Hamid Adila A.
Published: November 2023

Pre-eclampsia, which is part of the spectrum of hypertensive pregnancy disorders, poses a significant health burden, contributing to maternal and infant morbidity and mortality. Pre-eclampsia is widely associated with persistent adverse effects on the cardiovascular health of women with a history of pre-eclampsia. Additionally, there is increasing evidence demonstrating that offspring of pre-eclamptic pregnancies have altered cardiac structure and function, as well as different vascular physiology due to the decrease in endothelial function. Therefore, early detection of the likelihood of developing pre-eclampsia-associated cardiovascular diseases is vital, as this could facilitate the undertaking of the necessary clinical measures to avoid disease progression. The utilisation of microRNAs as biomarkers is currently on the rise as microRNAs have been found to play important roles in regulating various physiological and pathophysiological processes. In regard to pre-eclampsia, recent studies have shown that the expression of microRNAs is altered in postpartum women and their offspring who have been exposed to pre-eclampsia, and that these alterations may persist for several years. This review, therefore, addresses changes in microRNA expression found in postpartum women and offspring exposed to pre-eclampsia, their involvement in cardiovascular disease, and the potential role of microRNAs to be used as predictive tools and therapeutic targets in future cardiovascular disease research.
A Comparative Analysis of Selection Pressures Suffered by Mitochondrial Genomes in Two Planthopper Species with Divergent Climate Distributions
Authors: Sun Kang-Kang , Ding Yi , Chen Lei , Sun Jing-Tao
Published: November 2023

Mitochondrial DNA (mtDNA) has been widely used as a valuable tool in studies related to evolution and population genetics, under the implicit assumption of neutral evolution. However, recent studies suggest that natural selection also plays a significant role in shaping mitochondrial genome evolution, although the specific driving forces remain elusive. In this study, we aimed to investigate whether and how climate influences mitochondrial genome evolution by comparing the selection pressures acting on mitochondrial genomes between two rice planthoppers, Sogatella furcifera (Horváth) and Laodelphax striatellus (Fallén), which have different climate distributions. We employed the dN/dS method, MK test and Tajima’s D tests for our analysis. Our results showed that the mitochondrial genomes of the two species appear to undergo predominantly purifying selection, consistent with the nearly neutral evolution model. However, we observed varied degrees of purifying selection among the 13 protein-coding genes. Notably, ND1, ND2, ND6, COIII, and ATP8 exhibited significantly stronger purifying selection and greater divergence between the two species compared to the other genes. Additionally, we observed relatively stronger purifying selection in the mitochondrial genomes of S. furcifera compared to L. striatellus. This difference could be attributed to varying metabolic requirements arising from distinct habitats or other factors that are unclear here. Furthermore, we speculate that mito-nuclear epistatic interactions may play a role in maintaining nonsynonymous polymorphisms, particularly for COI and COII. Overall, our results shed some light on the influence of climate on mitochondrial genome evolution.
Enhanced Membrane Incorporation of H289Y Mutant GluK1 Receptors from the Audiogenic Seizure-Prone GASH/Sal Model: Functional and Morphological Impacts on Xenopus Oocytes
Authors: Díaz-Rodríguez Sandra M. , Ivorra Isabel , Espinosa Javier , Vegar Celia , Herrero-Turrión M. Javier , López Dolores E. , Gómez-Nieto Ricardo , Alberola-Die Armando
Published: November 2023

Epilepsy is a neurological disorder characterized by abnormal neuronal excitability, with glutamate playing a key role as the predominant excitatory neurotransmitter involved in seizures. Animal models of epilepsy are crucial in advancing epilepsy research by faithfully replicating the diverse symptoms of this disorder. In particular, the GASH/Sal (genetically audiogenic seizure-prone hamster from Salamanca) model exhibits seizures resembling human generalized tonic-clonic convulsions. A single nucleotide polymorphism (SNP; C9586732T, p.His289Tyr) in the Grik1 gene (which encodes the kainate receptor GluK1) has been previously identified in this strain. The H289Y mutation affects the amino-terminal domain of GluK1, which is related to the subunit assembly and trafficking. We used confocal microscopy in Xenopus oocytes to investigate how the H289Y mutation, compared to the wild type (WT), affects the expression and cell-surface trafficking of GluK1 receptors. Additionally, we employed the two-electrode voltage-clamp technique to examine the functional effects of the H289Y mutation. Our results indicate that this mutation increases the expression and incorporation of GluK1 receptors into an oocyte’s membrane, enhancing kainate-evoked currents, without affecting their functional properties. Although further research is needed to fully understand the molecular mechanisms responsible for this epilepsy, the H289Y mutation in GluK1 may be part of the molecular basis underlying the seizure-prone circuitry in the GASH/Sal model.
Endogenous Extracellular Vesicles Participate in Brain Remodeling after Ischemic Stroke
Authors: Alvarez Mauricio Muleiro , Salazar Felipe Esparza , Rodriguez Thomas , D’Egidio Francesco , Borlongan Cesar V. , Lee Jea-Young
Published: November 2023

Brain remodeling after an ischemic stroke represents a promising avenue for exploring the cellular mechanisms of endogenous brain repair. A deeper understanding of these mechanisms is crucial for optimizing the safety and efficacy of neuroprotective treatments for stroke patients. Here, we interrogated the role of extracellular vesicles, particularly exosomes, as potential mediators of endogenous repair within the neurovascular unit (NVU). We hypothesized that these extracellular vesicles may play a role in achieving transient stroke neuroprotection. Using the established ischemic stroke model of middle cerebral artery occlusion in adult rats, we detected a surged in the extracellular vesicle marker CD63 in the peri-infarct area that either juxtaposed or co-localized with GFAP-positive glial cells, MAP2-labeled young neurons, and VEGF-marked angiogenic cells. This novel observation that CD63 exosomes spatially and temporally approximated glial activation, neurogenesis, and angiogenesis suggests that extracellular vesicles, especially exosomes, contribute to the endogenous repair of the NVU, warranting exploration of extracellular vesicle-based stroke therapeutics.
Heterologous Expression of Two Brassica campestris CCCH Zinc-Finger Proteins in Arabidopsis Induces Cytoplasmic Foci and Causes Pollen Abortion
Authors: Xu Liai , Xiong Xingpeng , Liu Tingting , Cao Jiashu , Yu Youjian
Published: November 2023

The membrane-less organelles in cytoplasm that are presented as cytoplasmic foci were successively identified. Although multiple CCCH zinc-finger proteins have been found to be localized in cytoplasmic foci, the relationship between their specific localization and functions still needs further clarification. Here, we report that the heterologous expression of two Brassica campestris CCCH zinc-finger protein genes (BcMF30a and BcMF30c) in Arabidopsis thaliana can affect microgametogenesis by involving the formation of cytoplasmic foci. By monitoring the distribution of proteins and observing pollen phenotypes, we found that, when these two proteins were moderately expressed in pollen, they were mainly dispersed in the cytoplasm, and the pollen developed normally. However, high expression induced the assembly of cytoplasmic foci, leading to pollen abortion. These findings suggested that the continuous formation of BcMF30a/BcMF30c-associated cytoplasmic foci due to high expression was the inducement of male sterility. A co-localization analysis further showed that these two proteins can be recruited into two well-studied cytoplasmic foci, processing bodies (PBs), and stress granules (SGs), which were confirmed to function in mRNA metabolism. Together, our data suggested that BcMF30a and BcMF30c play component roles in the assembly of pollen cytoplasmic foci. Combined with our previous study on the homologous gene of BcMF30a/c in Arabidopsis, we concluded that the function of these homologous genes is conserved and that cytoplasmic foci containing BcMF30a/c may participate in the regulation of gene expression in pollen by regulating mRNA metabolism.
Changes of Ex Vivo Cervical Epithelial Cells Due to Electroporation with JMY
Authors: Halász Henriett , Szatmári Zoltán , Kovács Krisztina , Koppán Miklós , Papp Szilárd , Szabó-Meleg Edina , Szatmári Dávid
Published: November 2023

The ionic environment within the nucleoplasm might diverge from the conditions found in the cytoplasm, potentially playing a role in the cellular stress response. As a result, it is conceivable that interactions of nuclear actin and actin-binding proteins (ABPs) with apoptosis factors may differ in the nucleoplasm and cytoplasm. The primary intracellular stress response is Ca2+ influx. The junctional mediating and regulating Y protein (JMY) is an actin-binding protein and has the capability to interact with the apoptosis factor p53 in a Ca2+-dependent manner, forming complexes that play a regulatory role in cytoskeletal remodelling and motility. JMY’s presence is observed in both the cytoplasm and nucleoplasm. Here, we show that ex vivo ectocervical squamous cells subjected to electroporation with JMY protein exhibited varying morphological alterations. Specifically, the highly differentiated superficial and intermediate cells displayed reduced nuclear size. In inflamed samples, nuclear enlargement and simultaneous cytoplasmic reduction were observable and showed signs of apoptotic processes. In contrast, the less differentiated parabasal and metaplastic cells showed increased cytoplasmic activity and the formation of membrane protrusions. Surprisingly, in severe inflammation, vaginosis or ASC-US (Atypical Squamous Cells of Undetermined Significance), JMY appears to influence only the nuclear and perinuclear irregularities of differentiated cells, and cytoplasmic abnormalities still existed after the electroporation. Our observations can provide an appropriate basis for the exploration of the relationship between cytopathologically relevant morphological changes of epithelial cells and the function of ABPs. This is particularly important since ABPs are considered potential diagnostic and therapeutic biomarkers for both cancers and chronic inflammation.
Liposomal Encapsulation of Citicoline for Ocular Drug Delivery
Authors: Bonechi Claudia , Mahdizadeh Fariba Fahmideh , Talarico Luigi , Pepi Simone , Tamasi Gabriella , Leone Gemma , Consumi Marco , Donati Alessandro , Magnani Agnese
Published: November 2023

Glaucoma represents a group of neurodegenerative diseases characterized by optic nerve damage and the slowly progressive death of retinal ganglion cells. Glaucoma is considered the second leading cause of irreversible blindness worldwide. Pharmaceutical treatment of glaucoma is critical because of the properties of the ocular barrier that limit the penetration of drugs, resulting in lower systemic bioavailability. This behavior causes the need of frequent drug administration, which leads to deposition of concentrated solutions on the eye, causing toxic effects and cellular damage to the eye. To overcome these drawbacks, novel drug-delivery systems, such as liposomes, can play an important role in improving the therapeutic efficacy of antiglaucomatous drugs. In this work, liposomes were synthesized to improve various aspects, such as ocular barrier penetration, bioavailability, sustained release of the drug, targeting of the tissue, and reduction in intraocular pressure. Citicoline (CDP-choline; cytidine 5′-diphosphocholine) is an important intermediate in the biosynthesis of cell membrane phospholipids, with neuroprotective and neuroenhancement properties, and it was used in the treatment on retinal function and neural conduction in the visual pathways of glaucoma patients. In this study, citicoline was loaded into the 1,2-dioleoyl-sn-glycerol-3-phosphocholine and cholesterol liposomal carrier to enhance its therapeutic effect. The citicoline encapsulation efficiency, drug release, and size analysis of the different liposome systems were investigated using dynamic light scattering, nuclear magnetic resonance, infrared spectroscopy, and ToF-SIMS experiments.
MdMYB66 Is Associated with Anthocyanin Biosynthesis via the Activation of the MdF3H Promoter in the Fruit Skin of an Apple Bud Mutant
Authors: Huang Yaping , Li Wenfang , Jiao Shuzhen , Huang Juanjuan , Chen Baihong
Published: November 2023

Skin color is an important trait that is mainly determined by the content and composition of anthocyanins in apples. In this study, a new bud mutant (RM) from ‘Oregon Spur II’ (OS) of Red Delicious apple was obtained to reveal the mechanism underlying red color formation. Results showed that the total anthocyanin content in RM was significantly higher than that in OS with the development of fruit. Through widely-targeted metabolomics, we found that cyanidin-3-O-galactoside was significantly accumulated in the fruit skin of RM. Transcriptome analysis revealed that the structural gene MdF3H and MdMYB66 transcription factor were significantly up-regulated in the mutant. Overexpression of MdMYB66 in apple fruit and apple callus significantly promoted anthocyanin accumulation and significantly increased the expression level of MdMYB66 and structural genes related to anthocyanin synthesis. Y1H and LUC analysis verified that MdMYB66 could specifically bind to the promoter of MdF3H. The results of the double luciferase activity test showed that MdMYB66 activated MdF3H 3.8 times, which led to increased anthocyanin contents. This might explain the phenotype of red color in RM at the early stage. Taken together, these results suggested that MdMYB66 was involved in regulating the anthocyanin metabolic pathways through precise regulation of gene expression. The functional characterization of MdMYB66 provides insight into the biosynthesis and regulation of anthocyanins.
The Role of TGF-β during Pregnancy and Pregnancy Complications
Authors: Wen Baohong , Liao Huixin , Lin Weilin , Li Zhikai , Ma Xiaoqing , Xu Qian , Yu Feiyuan
Published: November 2023

Transforming growth factor beta (TGF-β), a multifunctional cytokine, is one of the most important inflammatory cytokines closely related to pregnancy. It plays significant roles in hormone secretion, placental development, and embryonic growth during pregnancy. TGF-β is implicated in embryo implantation and inhibits the invasion of extraepithelial trophoblast cells. It also moderates the mother-fetus interaction by adjusting the secretion pattern of immunomodulatory factors in the placenta, consequently influencing the mother’s immune cells. The TGF-β family regulates the development of the nervous, respiratory, and cardiovascular systems by regulating gene expression. Furthermore, TGF-β has been associated with various pregnancy complications. An increase in TGF-β levels can induce the occurrences of pre-eclampsia and gestational diabetes mellitus, while a decrease can lead to recurrent miscarriage due to the interference of the immune tolerance environment. This review focuses on the role of TGF-β in embryo implantation and development, providing new insights for the clinical prevention and treatment of pregnancy complications.
Oral Pyogenic Granuloma: A Narrative Review
Authors: Lomeli Martinez Sarah Monserrat , Carrillo Contreras Nadia Guadalupe , Gómez Sandoval Juan Ramón , Zepeda Nuño José Sergio , Gomez Mireles Juan Carlos , Varela Hernández Juan José , Mercado-González Ana Esther , Bayardo González Rubén Alberto , Gutiérrez-Maldonado Adrián Fernando
Published: November 2023

Pyogenic granuloma (PG) is a benign vascular lesion found predominantly in the oral cavity. Characterized by rapid growth and propensity to bleed, PG presents diagnostic challenges due to its similarity and alarming proliferation. This narrative review synthesizes current knowledge on the epidemiology, etiopathogenesis, clinical manifestations, and management of oral PG, with emphasis on recent advances in diagnostic and therapeutic approaches. The epidemiology of the injury is meticulously analyzed, revealing a higher incidence in women and a wide range of ages of onset. It delves into the etiopathogenesis, highlighting the uncertainty surrounding the exact causal factors, although historical attributions suggest an infectious origin. It exhaustively analyzes the clinical and histopathological aspects of oral PG, offering information on its various presentations and the importance of an accurate diagnosis to guide effective treatment. It details treatment strategies, emphasizing the personalized approach based on individual patient characteristics. This comprehensive review consolidates current knowledge on oral PG, highlighting the need for further research to clarify its pathogenesis and optimize treatment protocols.
Expression of Tissue Factor and Platelet/Leukocyte Markers on Extracellular Vesicles Reflect Platelet–Leukocyte Interaction in Severe COVID-19
Authors: Eichhorn Tanja , Weiss René , Huber Silke , Ebeyer-Masotta Marie , Mostageer Marwa , Emprechtinger Robert , Knabl Ludwig , Knabl Ludwig , Würzner Reinhard , Weber Viktoria
Published: November 2023

Severe COVID-19 is frequently associated with thromboembolic complications. Increased platelet activation and platelet–leukocyte aggregate formation can amplify thrombotic responses by inducing tissue factor (TF) expression on leukocytes. Here, we characterized TF-positive extracellular vesicles (EVs) and their cellular origin in 12 patients suffering from severe COVID-19 (time course, 134 samples overall) and 25 healthy controls. EVs exposing phosphatidylserine (PS) were characterized by flow cytometry. Their cellular origin was determined by staining with anti-CD41, anti-CD45, anti-CD235a, and anti-CD105 as platelet, leukocyte, red blood cell, and endothelial markers. We further investigated the association of EVs with TF, platelet factor 4 (PF4), C-reactive protein (CRP), and high mobility group box-1 protein (HMGB-1). COVID-19 patients showed higher levels of PS-exposing EVs compared to controls. The majority of these EVs originated from platelets. A higher amount of EVs in patient samples was associated with CRP, HMGB-1, PF4, and TF as compared to EVs from healthy donors. In COVID-19 samples, 16.5% of all CD41+ EVs displayed the leukocyte marker CD45, and 55.5% of all EV aggregates (CD41+CD45+) co-expressed TF, which reflects the interaction of platelets and leukocytes in COVID-19 on an EV level.
Equivalent Heat Treatments and Mechanical Properties in Cold-Rolled TiNiFe Shape-Memory Alloys
Authors: Liu Shuwei , Hui Songxiao , Li Yanfeng , Song Xiaoyun , Yu Yang , Ye Wenjun
Published: November 2023

Heat treatments after cold rolling for TiNiFe shape-memory alloys have been compared. After EBSD analysis and as calculated by the Avrami model and Arrhenius equation, the relationship between the heat-treatment temperature and manufacturing time of TiNiFe alloys is established. Through calculation, it can be found that TiNiFe alloys can obtain similar microstructures under the annealing processes of 823 K for 776 min, 827 K for 37 min, and 923 K for 12.5 min. And the recrystallization fractions are all around 50%. Nevertheless, the tensile properties and recovery stress of the alloys show almost similar values. And based on the feasibility of the annealing process, it is believed that annealing at 873 K for 37 min is the optimal choice to obtain a recrystallization fraction φR = 50%.
Zirconium Surface Treatment via Chemical Etching
Authors: Gołasz Przemysław , Kołkowska Agata , Zieliński Rafał , Simka Wojciech
Published: November 2023

The increased demand for implants that do not pose a threat to patients diagnosed using high-resolution magnetic resonance imaging and concerns arising from titanium allergies require the development of alternative implant materials. One promising concept is a use of zirconium as corrosion-resistant, nontoxic material that is lower in magnetic susceptibility. To achieve this, safe and efficient surface treatment methods of zirconium metal have to be developed. In this study, zirconium samples were treated with fluoride-free and fluoride-containing etching mixtures to determine their effect on the surface of Zr. SEM images were taken to investigate the preliminary effects of the etchants. Then, a second set of experiments was carried out using mixtures of HF-H2SO4 and ammonium persulfate–fluoride salts, as they gave the most promising results in the first trial. SEM images were taken and measurements on roughness, wettability, and atomic composition were made. The results showed an even zirconium surface in APS-fluoride salts, along with the formation of pits (1–3 μm) similar to those found in commercially available implants. There was no significant increase in the roughness of the treated samples. The addition of NO3− ions in the form of KNO3 speeded up etching and promoted pit formation. The HF-H2SO4 mixture was found to give unsatisfying results, as the surface was too rough and the formed pits were too large. It was concluded that etching zirconium in ammonium persulfate and fluoride salts is a promising area of research for the preparation of zirconium implants; however, further research has to be carried out on sandblasted samples.
Stromal Vascular Fraction Therapy for Knee Osteoarthritis: A Systematic Review
Authors: Goncharov Evgeniy Nikolaevich , Koval Oleg Aleksandrovich , Nikolaevich Bezuglov Eduard , Encarnacion Ramirez Manuel de Jesus , Engelgard Mikhail , Igorevich Eremin Ilya , Saporiti Alessandra , Valentinovich Kotenko Konstantin , Montemurro Nicola
Published: November 2023

Background and Objectives: Knee osteoarthritis (OA) is a widespread joint disease, set to increase due to aging and rising obesity. Beyond cartilage degeneration, OA involves the entire joint, including the synovial fluid, bones, and surrounding muscles. Existing treatments, such as NSAIDs and corticosteroid injections, mainly alleviate symptoms but can have complications. Joint replacement surgeries are definitive but carry surgical risks and are not suitable for all. Stromal vascular fraction (SVF) therapy is a regenerative approach using cells from a patient’s adipose tissue. SVF addresses as degenerative and inflammatory aspects, with potential for cartilage formation and tissue regeneration. Unlike traditional treatments, SVF may reverse OA changes. Being autologous, it reduces immunogenic risks. Materials and Methods: A systematic search was undertaken across PubMed, Medline, and Scopus for relevant studies published from 2017 to 2023. Keywords included “SVF”, “Knee Osteoarthritis”, and “Regenerative Medicine”. Results: This systematic search yielded a total of 172 articles. After the removal of duplicates and an initial title and abstract screening, 94 full-text articles were assessed for eligibility. Of these, 22 studies met the inclusion criteria and were subsequently included in this review. Conclusions: This review of SVF therapy for knee OA suggests its potential therapeutic benefits. Most studies confirmed its safety and efficacy, and showed improved clinical outcomes and minimal adverse events. However, differences in study designs and sizes require a careful interpretation of the results. While evidence supports SVF’s positive effects, understanding methodological limitations is key. Incorporating SVF is promising, but the approach should prioritize patient safety and rigorous research.
Correction: Gong et al. Neuroprotective and Cytotoxic Phthalides from Angelicae Sinensis Radix. Molecules 2016, 21, 549
Authors: Gong Wenxia , Zhou Yuzhi , Li Xiao , Gao Xiaoxia , Tian Junsheng , Qin Xuemei , Du Guanhua
Published: November 2023

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Macrophage-Immunomodulatory Actions of Bovine Whey Protein Isolate, Glycomacropeptide, and Their In Vitro and In Vivo Digests
Authors: Olsen Wyatt , Liang Ningjian , Dallas David C.
Published: November 2023

Whey protein isolate (WPI) consists of an array of proteins and peptides obtained as a byproduct of the cheesemaking process. Research suggests that WPI, along with its peptides such as glycomacropeptide (GMP), possesses immunomodulatory properties. These properties hold potential for alleviating the adverse effects of inflammatory conditions such as inflammatory bowel disease. Although promising, the immunoregulatory properties of the digested forms of WPI and GMP—those most likely to interact with the gut immune system—remain under-investigated. To address this knowledge gap, the current study examined the effects of in vitro-digested WPI and GMP, in vivo-digested WPI, and undigested WPI and GMP on the secretion of pro-inflammatory cytokines (TNF-α and IL-1β) in lipopolysaccharide-stimulated macrophage-like cells. Our results indicate that digested WPI and GMP reduced the expression of TNF-α and IL-1β, two pro-inflammatory cytokines. Whole WPI had no effect on TNF-α but reduced IL-1β levels. In contrast, in vivo-digested WPI reduced TNF-α but increased IL-1β. Undigested GMP, on the other hand, increased the secretion of both cytokines. These results demonstrate that digestion greatly modifies the effects of WPI and GMP on macrophages and suggest that digested WPI and GMP could help mitigate gastrointestinal inflammation. Further clinical studies are necessary to determine the biological relevance of WPI and GMP digestion products within the gut and their capacity to influence gut inflammation.
Analysis of Gut Characteristics and Microbiota Changes with Maternal Supplementation in a Neural Tube Defect Mouse Model
Authors: Cordero-Varela Juan Antonio , Reyes-Corral Marta , Lao-Pérez Miguel , Fernández-Santos Beatriz , Montenegro-Elvira Fernando , Sempere Lluis , Ybot-González Patricia
Published: November 2023

Adequate nutrient supply is crucial for the proper development of the embryo. Although nutrient supply is determined by maternal diet, the gut microbiota also influences nutrient availability. While currently there is no cure for neural tube defects (NTDs), their prevention is largely amenable to maternal folic acid and inositol supplementation. The gut microbiota also contributes to the production of these nutrients, which are absorbed by the host, but its role in this context remains largely unexplored. In this study, we performed a functional and morphological analysis of the intestinal tract of loop-tail mice (Vangl2 mutants), a mouse model of folate/inositol-resistant NTDs. In addition, we investigated the changes in gut microbiota using 16S rRNA gene sequencing regarding (1) the host genotype; (2) the sample source for metagenomics analysis; (3) the pregnancy status in the gestational window of neural tube closure; (4) folic acid and (5) D-chiro-inositol supplementation. We observed that Vangl2+/Lp mice showed no apparent changes in gastrointestinal transit time or fecal output, yet exhibited increased intestinal length and cecal weight and gut dysbiosis. Moreover, our results showed that the mice supplemented with folic acid and D-chiro-inositol had significant changes in their microbiota composition, which are changes that could have implications for nutrient absorption.
Development of Potential Multi-Target Inhibitors for Human Cholinesterases and Beta-Secretase 1: A Computational Approach
Authors: Barbosa Deyse B. , do Bomfim Mayra R. , de Oliveira Tiago A. , da Silva Alisson M. , Taranto Alex G. , Cruz Jorddy N. , de Carvalho Paulo B. , Campos Joaquín M. , Santos Cleydson B. R. , Leite Franco H. A.
Published: November 2023

Alzheimer’s disease causes chronic neurodegeneration and is the leading cause of dementia in the world. The causes of this disease are not fully understood but seem to involve two essential cerebral pathways: cholinergic and amyloid. The simultaneous inhibition of AChE, BuChE, and BACE-1, essential enzymes involved in those pathways, is a promising therapeutic approach to treat the symptoms and, hopefully, also halt the disease progression. This study sought to identify triple enzymatic inhibitors based on stereo-electronic requirements deduced from molecular modeling of AChE, BuChE, and BACE-1 active sites. A pharmacophore model was built, displaying four hydrophobic centers, three hydrogen bond acceptors, and one positively charged nitrogen, and used to prioritize molecules found in virtual libraries. Compounds showing adequate overlapping rates with the pharmacophore were subjected to molecular docking against the three enzymes and those with an adequate docking score (n = 12) were evaluated for physicochemical and toxicological parameters and commercial availability. The structure exhibiting the greatest inhibitory potential against all three enzymes was subjected to molecular dynamics simulations (100 ns) to assess the stability of the inhibitor-enzyme systems. The results of this in silico approach indicate ZINC1733 can be a potential multi-target inhibitor of AChE, BuChE, and BACE-1, and future enzymatic assays are planned to validate those results.
Developments in Scope and Availability of HF Radar Wave Measurements and Robust Evaluation of Their Accuracy
Authors: Wyatt Lucy R. , Green J. J.
Published: November 2023

HF radar systems form part of many operational coastal monitoring systems providing near-real-time surface currents for many useful applications. Although wave measurements have been possible with these systems for many years, they have not yet been adopted widely for operational monitoring because they have not been thought to be sufficiently accurate or reliable. However, the value of such data is beginning to be appreciated, and this is motivating more work on wave measurement with HF radar systems with many more papers on accuracy assessment and data availability appearing in the literature. In this paper, the wave measurement capability, limitations, and differences between different radar types are reviewed, and methods to assess accuracy are discussed and applied to phased array HF radar data obtained from the University of Plymouth WERA radars using the Seaview Software inversion method during April and November 2012 compared with directional buoy data. Good accuracy over a range of different wave parameters will be demonstrated. Newly available single-radar inversions are shown to be less accurate than dual-radar inversions, although they still provide useful data, and ways to improve performance are discussed. Swell and wind–sea components in the directional spectra are identified, and qualitative agreement with buoy peak parameters is demonstrated. Recommendations are given on statistical methods for the validation of wave parameters.
Satellite Velocity Correction Method of Ocean Current Retrieval for a Spaceborne Doppler Scatterometer
Authors: Zhang Jingyu , Dong Xiaolong , Zhu Di
Published: November 2023

For a spaceborne pencil-beam rotating Doppler scatterometer, its precision in measuring the ocean surface motion depends on the Doppler centroid of the received signals. The Doppler centroid is determined by the relative motion between the scatterometer and the ocean surface. This relative motion includes contributions from satellite velocity, the phase velocity of resonant Bragg waves, the orbital motions of ocean waves, and the ocean surface current. Subtracting the contribution of the satellite platform velocity from the complex Doppler information is important for the application of a spaceborne Doppler scatterometer in ocean surface current retrieval. In this research, we propose a method for the platform velocity correction influenced by the Doppler centroid offset and analyze the accuracy of this correction method. The method is based on the echoed signal model of a Doppler scatterometer. Our results show that the offset could lead to a measurement offset of up to 0.02 m/s when the beam width was 0.3°. For a 0.6° beam width, the maximum offset was 0.07 m/s. Thus, with the high accuracy of the current spaceborne sensors’ measurement, the offset can be accurately eliminated. In future applications and data processing algorithms, this effect should be considered.
A Flexible System-on-Chip Field-Programmable Gate Array Architecture for Prototyping Experimental Global Navigation Satellite System Receivers
Authors: Majoral Marc , Fernández-Prades Carles , Arribas Javier
Published: November 2023

Global navigation satellite system (GNSS) technology is evolving at a rapid pace. The rapid advancement demands rapid prototyping tools to conduct research on new and innovative signals and systems. However, researchers need to deal with the increasing complexity and integration level of GNSS integrated circuits (IC), resulting in limited access to modify or inspect any internal aspect of the receiver. To address these limitations, the authors designed a low-cost System-on-Chip Field-Programmable Gate Array (SoC-FPGA) architecture for prototyping experimental GNSS receivers. The proposed architecture combines the flexibility of software-defined radio (SDR) techniques and the energy efficiency of FPGAs, enabling the development of compact, portable, multi-channel, multi-constellation GNSS receivers for testing novel and non-standard GNSS features with live signals. This paper presents the proposed architecture and design methodology, reviewing the practical application of a spaceborne GNSS receiver and a GNSS rebroadcaster, and introducing the design and initial performance evaluation of a general purpose GNSS receiver serving as a testbed for future research. The receiver is tested, demonstrating the ability of the receiver to acquire and track GNSS signals using static and low Earth orbit (LEO)-scenarios, assessing the observables’ quality and the accuracy of the navigation solutions.
Coordination Analysis of Sustainable Agricultural Development in Northeast China from the Perspective of Spatiotemporal Relationships
Authors: Yang Guang , Yan Hua , Li Quanfeng
Published: November 2023

The coordination of sustainable agricultural development is essential for optimizing agricultural policies and ensuring food security. However, only a few studies have examined the temporal and spatial aspects of the coordination of sustainable agricultural development systems in Northeast China. This study proposed a theoretical framework based on the dissipative structure theory, which suggests that the coordination among the four subsystems of economy, society, resources, and technology plays a crucial role in determining the level of sustainable agricultural development in Northeast China (SADINC). Then, the present study took socioeconomic statistical data as data sources and integrated administrative division data using the ArcGIS platform, forming spatial data for municipal-level units of SADINC from 2000 to 2020. The entropy weight method was applied to analyze the change in sustainable agricultural development, while the coordination degree model was used to analyze the coordination among different subsystems. The main results showed a general increase in the index of SADINC. The central part of the study area generally exhibits higher urban economic and resource indices, while the southern region exhibits higher urban social and technological indices. The average coordination degree of SADINC decreased from 1.736 to 1.639, representing a decline of 0.097. Moreover, the spatial polarization in most cities’ coordination degrees and subsystem indicators is not pronounced. However, there are characteristics of high-high agglomeration and low-low agglomeration. The high-high aggregation of the coordination degree shows a dispersal pattern from the large-scale agglomeration in the south-east to the central part. The distribution of low-low agglomeration transforms from large-scale agglomeration in the west to small-scale agglomeration in the northeast. The implementation of agricultural policies has dramatically impacted the increase in the index of each subsystem in Northeast China from 2000 to 2022. However, it is crucial to ensure coordination among the subsystems. Therefore, future policies should focus on strengthening the coordination among the economic, social, resource, and technology subsystems to achieve sustainable agricultural development.
Identifying Trends in the Vegetation Distribution of Climate-Sensitive Species in the Korean Peninsula, Based on Climate Attributes
Authors: Shin Yeeun , Shin Eunseo , Lee Sang-Woo , An Kyungjin
Published: November 2023

This study analyzed the trends and changes in climate-sensitive plants based on environmental attributes, using on-site survey data collected between 2006 and 2018. In combination with environmental attributes, 16 plant species were chosen as climate-sensitive biological indicator species and were spatially distributed based on national ecological survey data. With the use of regression methods and locally weighted scatterplot smoothing, possible changes in the vegetation distribution trends were simulated. The findings indicated that annual rainfall and temperature are critical environmental attributes, as they mostly affect the plant ecosystem. The simulated results suggest that plant habitats for the selected 16 individual species tend to be found in higher temperature zones, with a few exceptions. However, there was no clear pattern of the effects of annual rainfall on climate-sensitive plant species. The effects of climate change on these species are a risk to the environment; however, depending on the situation, adequate adaptation and management plans could provide opportunities for policy makers to make evidence-based decisions.
Economic Evaluation and Technoeconomic Resilience Analysis of Two Routes for Hydrogen Production via Indirect Gasification in North Colombia
Authors: González-Delgado Ángel Darío , Vargas-Mira Alexander , Zuluaga-García Carlos
Published: November 2023

Hydrogen has become a prospective energy carrier for a cleaner, more sustainable economy, offering carbon-free energy to reduce reliance on fossil fuels and address climate change challenges. However, hydrogen production faces significant technological and economic hurdles that must be overcome to reveal its highest potential. This study focused on evaluating the economics and technoeconomic resilience of two large-scale hydrogen production routes from African palm empty fruit bunches (EFB) by indirect gasification. Computer-aided process engineering (CAPE) assessed multiple scenarios to identify bottlenecks and optimize economic performance indicators like gross profits, including depreciation, after-tax profitability, payback period, and net present value. Resilience for each route was also assessed, considering raw material costs and the market price of hydrogen in relation to gross profits and after-tax profitability. Route 1 achieved a gross profit (DGP) of USD 47.12 million and a profit after taxes (PAT) of USD 28.74 million, while Route 2 achieved a DGP of USD 46.53 million and a PAT of USD 28.38 million. The results indicated that Route 2, involving hydrogen production through an indirect gasification reactor with a Selexol solvent unit for carbon dioxide removal, demonstrated greater resilience in terms of raw material costs and product selling price.
Safety–Security Analysis of Maritime Surveillance Systems in Critical Marine Areas
Authors: Şengül Batu , Yılmaz Fatih , Uğurlu Özkan
Published: November 2023

In today’s world, wherein more than 80% of world trade is carried out by maritime routes, the safety and security of the seas where this trade takes place is of vast importance for nations and the international community. For this reason, ensuring the sustainable safety and security of the seas has become an integral part of the mission of all maritime-related entities. Using big data extracted from the seas and maritime activities into meaningful information with artificial intelligence applications and developing applications that can be used in maritime surveillance will be of great importance for augmenting maritime safety and security. In this article, data sources which can be used by a maritime surveillance system based on big data and artificial intelligence technologies and which can be established around sensitive sea areas and critical coastal facilities, are identified and a model proposal using this maritime big data is put forward.
The Effect of Electronic Human Resource Management Systems on Sustainable Competitive Advantages: The Roles of Sustainable Innovation and Organizational Agility
Authors: Alqarni Khalid , Agina Mohamed Fathy , Khairy Hazem Ahmed , Al-Romeedy Bassam Samir , Farrag Dalia Abdelrahman , Abdallah Rabab Mahmoud
Published: November 2023

This study attempts to examine the impact of electronic human resource management (E-HRM) systems on achieving a sustainable competitive advantage (SCA) in the tourism and hospitality industry by incorporating sustainable innovation (SI) and organizational agility (OA) as mediators. To survive, tourist and hospitality organizations must embrace digital transformation by using E-HRM practices such as digital recruiting, online training, and performance management tools. Organizations may improve their HR departments’ efficiency, effectiveness, and decision making, enhancing the overall organizational performance and preserving a sustainable competitive edge. Middle and first-line management of the five-star hotels and travel agencies in category A operating in Egypt were investigated. A PLS-SEM analysis was performed on 313 valid responses, gathered using WarpPLS 7.0. The findings revealed that E-HRM is positively associated with SCA, SI, and OA. Furthermore, there were positive associations between SI and OA and SCA. SI and OA were also shown to be mediators in the relationship between E-HRM and SCA. The study adds to the existing literature on how E-HRM impacts SI, OA, and SCA. The relationship between these components in hotels and travel agencies has not been the subject of any empirical research. The current study has addressed gaps by empirically analyzing these relationships in the context of Egyptian hotels and travel agencies. It integrates E-HRM, innovation, organizational agility, and competitive advantage within the context of sustainability in tourism and hospitality.