bluuebunny/crossref_metadata_2025 huggingface dataset:
Association of the timing of evening eating with BMI Z-score and waist-to-height ratio among preschool-aged children in Finland
Authors: Rahkola Jenna , Lehtimäki Aku-Ville , Abdollahi Anna M. , Merikanto Ilona , Vepsäläinen Henna , Björkqvist Josefine , Roos Eva , Erkkola Maijaliisa , Lehto Reetta
Published: October 2023

Later timing of eating has been associated with higher adiposity among adults and children in several studies, but not all. Moreover, studies in younger children are scarce. Hence, this study investigated the associations of the timing of evening eating with BMI Z-score and waist-to-height ratio (WHtR), and whether these associations were moderated by chronotype among 627 preschoolers (3–6-year-olds) from the cross-sectional DAGIS survey in Finland. Food intake was measured with 3-d food records, and sleep was measured with hip-worn actigraphy. Three variables were formed to describe the timing of evening eating: (1) clock time of the last eating occasion (EO); (2) time between the last EO and sleep onset; and (3) percentage of total daily energy intake (%TDEI) consumed 2 h before sleep onset or later. Chronotype was assessed as a sleep debt-corrected midpoint of sleep on the weekend (actigraphy data). The data were analysed with adjusted linear mixed effects models. After adjusting for several confounders, the last EO occurring closer to sleep onset (estimate = −0·006, 95 % CI (−0·010, −0·001)) and higher %TDEI consumed before sleep onset (estimate = 0·0004, 95 % CI (0·00003, 0·0007)) were associated with higher WHtR. No associations with BMI Z-score were found after adjustments. Clock time of the last EO was not significantly associated with the outcomes, and no interactions with chronotype emerged. The results highlight the importance of studying the timing of eating relative to sleep timing instead of only as clock time.
How the Gospel of Truth Depicts Paul’s Secret Teaching: A Study in Second-Century Reception History
Authors: Pagels Elaine
Published: October 2023

This article shows that the Gospel of Truth (NHC I, 3), dense with allusions to sources now in the New Testament, most often explored for its resonances with Johannine literature, also offers significant evidence for second-century reception of Paul’s letters, while highlighting poetic images often overlooked. Correlating the language and literary structure of such Pauline passages as 1 Cor 1–6 with the opening of the Gospel of Truth shows that the latter implicitly claims to reveal the secret and primordial “wisdom of God” that Paul declares he teaches only orally to initiates (1 Cor 2:6–7). Thus, this text exemplifies a kind of “heretical” reading that heresiologists like Irenaeus deplore, when, for example, he cites this very passage to complain that “each of (the heretics) declares that this ‘wisdom’ is whatever he invents (fictionem videlicet), so that sometimes they claim that the truth is in Valentinus, or in Marcion, or in someone else …” (Haer. 3.2.1). Furthermore, this research suggests that the Gospel of Truth, narrating primordial creation, followed by a dramatic account of Christ’s incarnation and redemption, claims to offer, as the “true gospel,” a spiritual interpretation that far transcends the brief kerygmatic version set forth in 1 Cor 15:1–3. Finally, I suggest, investigating the Gospel of Truth’s interpretation of Paul’s teaching (which here includes echoes of Ephesians and Colossians) invites us to recognize elements of Paul’s letters most often overlooked. For, from patristic times to the present, exegetes who accept Irenaeus’s insistence that Paul had no secret teaching have dismissed the apostle’s emphatic claim that he did. Furthermore, those focused on dogmatic and ethical themes in Paul’s letters often miss poetic and mythological language at play in sources like the Gospel of Truth.
The Tana-Beles Project in Ethiopia and the Making of Postcolonial Humanitarianism, 1938–1994
Authors: Salvatici Silvia
Published: October 2023

This article takes the example of the Tana-Beles project – a scheme sponsored by Italy to respond to the 1980s famine in Ethiopia – to demonstrate that postimperial international relief policies and practices were woven into the very fabric of the colonial past. Postcolonial humanitarianism emerges as the transformation of colonial practices and relationships into new policies, which did not depend on the interests only of former metropoles, but also of the new independent states and on the agenda of international organizations. Furthermore, this article contends that private companies had a prominent role in shaping postcolonial humanitarianism because they could benefit from both the favourable policies of donor countries and the relationships they established in the long run with the authorities of recipient countries. Finally, the history of the Tana-Beles project enables us to re-read the international response to the Ethiopian famine within a larger timeframe. On the one hand, this appears to be rooted in the previous colonial period, on the other it embraces the years thereafter when the schemes, relationships, and strategies set in motion during the famine were developed further.
An adjoint-based method for optimising MHD equilibria against the infinite-n, ideal ballooning mode
Authors: Gaur Rahul , Buller Stefan , Ruth Maximilian E. , Landreman Matt , Abel Ian G. , Dorland William D.
Published: October 2023

We demonstrate a fast adjoint-based method to optimise tokamak and stellarator equilibria against a pressure-driven instability known as the infinite-

$n$

ideal ballooning mode. We present three finite-

$\beta$

(the ratio of thermal to magnetic pressure) equilibria: one tokamak equilibrium and two stellarator equilibria that are unstable against the ballooning mode. Using the self-adjoint property of ideal magnetohydrodynamics, we construct a technique to rapidly calculate the change in the eigenvalue, a measure of ideal ballooning instability. Using the SIMSOPT optimisation framework, we then implement our fast adjoint gradient-based optimiser to minimise the eigenvalue and find stable equilibria for each of the three originally unstable equilibria.
Harnessing the power on our plates: sustainable dietary patterns for public and planetary health
Authors: Woodside Jayne V. , Lindberg Leona , Nugent Anne P.
Published: October 2023

Globally, diet quality is poor, with populations failing to achieve national dietary guidelines. Such failure has been consistently linked with malnutrition and poorer health outcomes. In addition to the impact of diet on health outcomes, it is now accepted that what we eat, and the resulting food system, has significant environmental or planetary health impacts. Changes are required to our food systems to reduce these impacts and mitigate the impact of climate change on our food supply. Given the complexity of the interactions between climate change, food and health, and the different actors and drivers that influence these, a systems-thinking approach to capture such complexity is essential. Such an approach will help address the challenges set by the UN 2030 Agenda for sustainable development in the form of the sustainable development goals (SDG). Progress against SDG has been challenging, with an ultimate target of 2030. While the scientific uncertainties regarding diet and public and planetary health need to be addressed, equal attention needs to be paid to the structures and systems, as there is a need for multi-level, coherent and sustained structural interventions and policies across the full food system/supply chain to effect behaviour change. Such systems-level change must always keep nutritional status, including impact on micronutrient status, in mind. However, benefits to both population and environmental health could be expected from achieving dietary behaviour change towards more sustainable diets.
Duty, Virtue, and Filial Love
Authors: Um Sungwoo
Published: October 2023

The aim of this paper is to argue that the normative significance of the inner aspects of filial piety – in particular, filial love – is better captured when we understand filial love as part of the virtue of filial piety rather than as an object of duty. After briefly introducing the value of filial love, I argue that the idea of a duty to love one's loving parents faces serious difficulties in making sense of the normative significance of filial love. Then I show why the virtue-ethical approach to filial love, which views filial love as a constitutive part of the virtue of filial piety, can do justice to its normative significance while avoiding the difficulties.
Plays with words: Fungible(ly) fugitive Black sound in ethnographies of communication
Authors: Pannell Justin Lance
Published: October 2023

Anti-Black language ideologies manifest in exclusionary language policies (e.g. Sung & Allen-Handy 2019), educational tracking (e.g. Sung 2018), and scholarly claims of Black ‘deficiency’ (Smitherman 2000). A liberal educational research tradition has countered with ethnographic accounts of cultural ‘mismatch’ (Michaels 2006) vis-à-vis Black educational ‘failure’. Conducting a textual analysis of an archive of ethnography of communication texts, I locate multiple genealogical linkages holding between ‘mismatch’ and deprivation discourses, principally ones centered on representations of ‘pathological’ Black ‘matriarchy’. Paralleling Black feminist theorizations of ‘fungible Black flesh’ (e.g. Hartman 1997), I account for these representations by conceptualizing ‘fungible Black sound’. I further argue that ‘fungible fugitivity’ (e.g. Snorton 2017), that is, how Blackness fluidly responds to white incursion is linguistically realized in acts of ‘signifyin(g)’ (e.g. Mitchell-Kernan 1999), yielding the analytic category ‘fungible(ly) fugitive Black sound’. Lastly, I reread an ethnographic text with this analytic to illustrate its affordance for (re)imagining Black futurity. (Black sound, fungibility, fugitivity, raciolinguistic ideologies, ethnography of communication, Black studies)*
Depressive and anxiety symptom trajectories in Polish adolescents during the COVID-19 pandemic and the outbreak of war in Ukraine: uncovering the role of family relations
Authors: Gambin Małgorzata , Wnuk Anna , Oleksy Tomasz , Sękowski Marcin , Kubicka Karolina , Woźniak-Prus Małgorzata , Sharp Carla , Bonanno George A.
Published: October 2023

Introduction:

The aim of the study was to investigate longitudinal trajectories of change in anxiety and depression symptoms in Polish adolescents during the second year of the COVID-19 pandemic and after the outbreak of the war in Ukraine. Additionally, we aimed to identify risk/protective factors and outcomes associated with these trajectories.

Method:

We collected data in three waves between November 2021 and May 2022. Adolescents (N = 281 in the first wave) completed the Patient Health Questionnaire-9, the Generalized Anxiety Disorder-7, the Filial Responsibility Scale for Youth, and questions related to the COVID-19 pandemic and war in Ukraine.

Results:

We identified three trajectories of depressive symptoms: resilient with low, stable symptoms (71% of participants), chronically elevated symptoms (11%), and acute symptoms followed by recovery (18%). We distinguished two trajectories of anxiety symptoms: resilient (75%) and chronic (25%). Non-resilient trajectories were predicted by higher levels of familial unfairness (perceived lack of equality and reciprocity in the family), relationship difficulties at school and at home, older age, and poor socioeconomic status. Chronic depressive and anxiety symptoms were associated with higher war-related concerns.

Discussion:

These findings can inform preventive and therapeutic interventions for at-risk adolescents to reduce negative long-term outcomes of social crises.
Children’s resilience to sibling victimization: The role of family, peer, school, and neighborhood factors
Authors: Sellars Elise , Oliver Bonamy R. , Bowes Lucy
Published: October 2023

Although common, little is known about the potential impacts of sibling victimization, and how best to ameliorate these. We explored longitudinal associations between sibling victimization and mental health and wellbeing outcomes, and promotive and risk factors that predicted better or worse outcomes following victimization. Data were from >12,000 participants in the Millennium Cohort Study, a longitudinal UK birth cohort, who reported on sibling victimization at age 11 and/or 14 years. We identified potential risk and promotive factors at family, peer, school, and neighborhood levels from age 14 data. Mental health and wellbeing outcomes (internalizing and externalizing problems, mental wellbeing, self-harm) were collected at age 17. Results suggested that over and above pre-existing individual and family level vulnerabilities, experiencing sibling victimization was associated with significantly worse mental health and wellbeing. Having no close friends was a risk factor for worse-than-expected outcomes following victimization. Higher levels of school motivation and engagement was a promotive factor for better-than-expected outcomes. This indicates that aspects of the school environment may offer both risk and promotive factors for children experiencing sibling victimization at home. We argue that effective sibling victimization interventions should be extended to include a focus on factors at the school level.
The relative effects of parental alcohol use disorder and maltreatment on offspring alcohol use: Unique pathways of risk
Authors: Ross Andrew J. , Russotti Justin , Toth Sheree L. , Cicchetti Dante , Handley Elizabeth D.
Published: October 2023

Childhood adversity represents a robust risk factor for the development of harmful substance use. Although a range of empirical studies have examined the consequences of multiple forms of adversity (i.e., childhood maltreatment, parental alcohol use disorder [AUD]), there is a dearth of information on the relative effects of each form of adversity when considered simultaneously. The current study utilizes structural equation modeling to investigate three unique and amplifying pathways from parental AUD and maltreatment exposure to offspring alcohol use as emerging adults: (1) childhood externalizing symptomatology, (2) internalizing symptomatology, and (3) affiliation with substance-using peers and siblings. Participants (N = 422) were drawn from a longitudinal follow-up study of emerging adults who participated in a research summer camp program as children. Wave 1 of the study included 674 school-aged children with and without maltreatment histories. Results indicated that chronic maltreatment, over and above the effect of parent AUD, was uniquely associated with greater childhood conduct problems and depressive symptomatology. Mother alcohol dependence was uniquely associated with greater affiliation with substance-using peers and siblings, which in turn predicted greater alcohol use as emerging adults. Results support peer and sibling affiliation as a key mechanism in the intergenerational transmission of substance use between mothers and offspring.
Exploring feasibility and acceptability of an integrated urban gardens and peer nutritional counselling intervention for people with HIV in the Dominican Republic
Authors: Celeste-Villalvir Alane , Then-Paulino Amarilis , Armenta Gabriela , Jimenez-Paulino Gipsy , Palar Kartika , Wallace Deshira D , Derose Kathryn P
Published: October 2023

Objective:

Food security interventions with people living with HIV (PLHIV) are needed to improve HIV outcomes. This process evaluation of a pilot intervention involving urban gardening and peer nutritional counselling with PLHIV assesses feasibility, acceptability and implementation challenges to inform scale-up.

Design:

Mixed methods were used, including quantitative data on intervention participation and feasibility and acceptability among participants (n 45) and qualitative data from a purposive sample of participants (n 21). Audio-recorded interviews were transcribed and coded using a codebook developed iteratively.

Setting:

An HIV clinic in the northwest-central part of the Dominican Republic.

Results:

The intervention was feasible for most participants: 84 % attended a garden workshop and 71 % established an urban garden; 91 % received all three core nutritional counselling sessions; and 73 % attended the cooking workshop. The intervention was also highly acceptable: nearly, all participants (93–96 %) rated the gardening as ‘helpful’ or ‘very helpful’ for taking HIV medications, their mental/emotional well-being and staying healthy; similarly, high percentages (89–97 %) rated the nutrition counselling ‘helpful’ or ‘very helpful’ for following a healthy diet, reducing unhealthy foods and increasing fruit/vegetable intake. Garden barriers included lack of space and animals/pests. Transportation barriers impeded nutritional counselling. Harvested veggies were consumed by participants’ households, shared with neighbours and family, and sold in the community. Many emphasised that comradery with other PLHIV helped them cope with HIV-related marginalisation.

Conclusion:

An urban gardens and peer nutritional counselling intervention with PLHIV was feasible and acceptable; however, addressing issues of transportation, pests and space is necessary for equitable participation and benefit.
Understanding the Inflation and Social Policy Nexus
Authors: Béland Daniel , Cantillon Bea , Greve Bent , Hick Rod , Moreira Amilcar
Published: October 2023

The cost-of-living crisis that began in the aftermath of the COVID-19 crisis and the attempted Russian invasion of Ukraine has major implications for social policy. In advanced industrial countries, this is the most dramatic cost-of-living crisis since the mid-late 1970s and early 1980s. In this contribution, we explore the inflation and social policy nexus to identify the nature and sources of inflation, its redistributive and policy implications, and the specific nature of the current cost-of-living crisis compared to two other recent crises: the 2008 financial crisis and the COVID-19 pandemic. Focusing on advanced industrial countries and drawing on the available scholarship about these topics, we offer the background necessary to understand the challenges facing welfare states in times of dramatically high inflation. As a way to provide broad context to the present themed section, our discussion stresses the economic, social, and political dynamics shaping social policy adaptation to inflationary pressures.
The Cost-Effectiveness of Psychosocial Interventions Following Self-Harm in Australia
Authors: Krysinska Karolina , Andriessen Karl , Bandara Piumee , Reifels Lennart , Flego Anna , Page Andrew , Schlichthorst Marisa , Pirkis Jane , Mihalopoulos Cathrine , Khanh-Dao Le Long
Published: October 2023

Abstract: Background: Psychosocial interventions following self-harm in adults, in particular cognitive behavioral therapy (CBT), can be effective in lowering the risk of repeated self-harm. Aims: To evaluate the cost-effectiveness of CBT for reducing repeated self-harm in the Australian context. Method: The current study adopted the accessing cost-effectiveness (ACE) approach using return-on-investment (ROI) analysis. Uncertainty and sensitivity analyses (Sas) tested the robustness of the model outputs to changes in three assumptions: general practitioner referral pathway (SA1), private setting intervention delivery (SA2), and training costs (SA3). Results: The intervention produced cost savings of A$ 46M (95% UI −223.7 to 73.3) and A$ 18.3M (95% UI −86.2 to 24.6), subject to the effect of intervention lasting 2- or 1-year follow-up. The ROI ratio reduced to 5.22 in SA1 (95% UI −10.1 to 27.9), 2.5 in SA2 (95% UI −4.8 to 13.3), and 5.1 in SA3 (95% UI −9.8 to 27.8). Limitations: We assumed that the effectiveness would reduce 50% within 5 years in the base case, and we used Australian data and a partial social perspective. Conclusions: The current study demonstrated cost-effectiveness of CBT for adults who have self-harmed with the return-on-investment ratio of A$ 2.3 to $6.0 for every A$ 1 invested.
Tropical Precipitation Woes in the Community Earth System Model Version 2
Authors: Lofverstrom Marcus , Zhu Jiang
Published: October 2023

The newly developed paleo‐climate calibrated Community Earth System Model, version 2 (pCESM2) simulates a more realistic global temperature response to external forcing compared to the standard CESM2. Here we show that the code modifications in pCESM2 result in increased atmospheric convection and a northward shift of the Atlantic and eastern Pacific Intertropical Convergence Zones. These changes are exacerbated under Last Glacial Maximum forcing, resulting in tropical precipitation changes that are inconsistent with both proxy data evidence and simulations with other contemporary models. Similar model‐data disagreements are also present in the standard CESM2. Thus, more work is needed to improve the simulated Last Glacial Maximum hydroclimate response in CESM2. We further suggest that well‐constrained paleo climates should be given a larger emphasis in model development more broadly, as these climates can help identify issues with model parameterizations under altered forcing and thus improve the fidelity of simulations of past, present, and future climates.
Observation of Whistler Mode Waves Inside Mirror Mode Structures in the Earth's Outer Magnetosphere
Authors: Chen Rui , Gao Xinliang , Lu Quanming , Tsurutani Bruce T. , Miyoshi Yoshizumi , Zhou Xuan , Ke Yangguang , Chen Huayue , Ma Jiuqi
Published: October 2023

Whistler mode waves are commonly observed inside mirror mode structures (MM‐Ss) in the magnetosheath. Although MM‐Ss have also been detected in the magnetosphere, there is no statistical study investigating the properties of whistler mode waves inside MM‐Ss. Using Magnetospheric Multiscale (MMS) satellites, our present study identifies and statistically analyzes whistler mode waves detected inside MM‐Ss in the Earth's magnetosphere. Both the observational evidence (bidirectional propagation) and theoretical analyses suggest that whistler mode waves are excited in the low‐magnetic‐field regions of MM‐Ss. Statistical results indicate that whistler mode waves are frequently observed inside the MM‐Ss in the dusk sector. Most of these waves (>70%) are in the frequency range of 0.1–0.4 fce and have amplitudes less than ∼50 pT. Moreover, most of them are observed to propagate in the direction both parallel and antiparallel to the background magnetic field, and their wave normal angles are typically less than ∼40°. Our study analyzes the properties and generation of whistler mode waves inside MM‐Ss and reveals that MM‐Ss are a possible source region in the Earth's magnetosphere. Therefore, our study provides new insight into the properties of whistler mode waves inside MM‐Ss which are pervasive in space plasmas.
Radiatively Active Hydrometeor Frequencies From CloudSat–CALIPSO Data for Evaluating Cloud Fraction in Global Climate Models
Authors: Li Jui‐Lin F. , Lee Wei‐Liang , Xu Kuan‐Man , Tsai Yu‐Cian , Jiang Jonathan , Yu Jia‐Yuh , Stephens Graeme , Fetzer Eric , Chen Wei‐Ting
Published: October 2023

This study derives radiatively active hydrometeor frequencies (HFs) from CloudSat–CALIPSO satellite data to evaluate modeled cloud fraction in recent historical period simulations by the fifth phase of the Coupled Model Intercomparison Project (CMIP5) models. Most CMIP5 models do not consider precipitating and convective core hydrometeors in radiative transfer calculation except for CESM1‐CAM5 with precipitating ice (snow). They do not provide radiatively active cloud fractions for all precipitating hydrometeor types such as snow fraction while the satellite‐retrieved HFs include mixed profiles of stratiform clouds and precipitation as well as convective cores. A filtering method is applied to produce estimates of nonprecipitating and nonconvective core HF (NPCHF) from the total HF (THF) excluding rain, which is defined here as the sum of NPCHF, precipitating ice (snow, graupel, and hail), and convective core HF. We determine the optimal reference HF data for model comparison that include estimates of liquid‐phase NPCHF from CloudSat radar‐only data (2B‐CWC) and ice‐phase THF from CloudSat–CALIPSO 2C‐ICE combined radar/lidar data. The zonal‐mean at selected heights and regionally averaged profiles of CMIP5 multi‐model‐mean cloud fraction show good agreements in comparison with NPCHF estimates with biases within 5%, whereas cloud fraction is significantly underestimated (up to 30%) against the THF estimates. The biases appear below the midtroposphere over the extratropics, in the upper troposphere over the midlatitude lands and a few tropical convective regions, where precipitating ice and convective core hydrometeors contribute greatly. This comparison reveals some underestimates of thick cloud HFs resulting from measurement limitations due probably to attenuation of radar/lidar signals, which cannot be qualified in this study.
A Series of Advances in Analytic Interplanetary CME Modeling
Authors: Kay C. , Nieves‐Chinchilla T. , Hofmeister S. J. , Palmerio E. , Ledvina V. E.
Published: October 2023

Coronal mass ejections (CMEs) and high speed streams (HSSs) are large‐scale transient structures that routinely propagate away from the Sun. Individually, they can cause space weather effects at the Earth, or elsewhere in space, but many of the largest events occur when these structures interact during their interplanetary propagation. We present the initial coupling of Open Solar Physics Rapid Ensemble Information (OSPREI), a model for CME evolution, with Mostly Empirical Operational Wind with a High Speed Stream, a time‐dependent HSS model that can serve as a background for the OSPREI CME. We present several improvements made to OSPREI in order to take advantage of the new time‐dependent, higher‐dimension background. This includes an update in the drag calculation and the ability to determine the rotation of a yaw‐like angle. We present several theoretical case studies, describing the difference in the CME behavior between a HSS background and a quiescent one. This behavior includes interplanetary CME propagation, expansion, deformation, and rotation, as well as the formation of a CME‐driven sheath. We also determine how the CME behavior changes with the HSS size and initial front distance. Generally, for a fast CME, we see that the drag is greatly reduced within the HSS, leading to faster CMEs and shorter travel times. The drag reappears stronger if the CME reaches the stream interaction region or upstream solar wind, leading to a stronger shock with more compression until the CME sufficiently decelerates. We model a CME–HSS interaction event observed by Parker Solar Probe in January 2022. The model improvements create a better match to the observed in situ profiles.
Multi-dose enteral L-citrulline administration in premature infants at risk of developing pulmonary hypertension associated with bronchopulmonary dysplasia
Authors: Fike Candice D. , Aschner Judy L. , Avachat Charul , Birnbaum Angela K. , Sherwin Catherine M. T.
Published: October 2023

Objective

Information is needed to guide the design of randomized controlled trials (RCTs) evaluating L-citrulline therapy for premature infants with pulmonary hypertension associated with bronchopulmonary dysplasia (BPD-PH). Based on our single-dose pharmacokinetic study, we evaluated the ability of a multi-dose enteral L-citrulline strategy to achieve a target trough steady-state L-citrulline plasma concentration and its tolerability in premature infants.

Study Design

Plasma L-citrulline concentrations were measured in six premature infants receiving 60 mg/kg L-citrulline every 6 h for 72 h before the first and last L-citrulline doses. L-citrulline concentrations were compared to concentration-time profiles from our previous study.

Results

Target trough plasma L-citrulline concentrations were achieved in 2/6 subjects. No serious adverse events occurred.

Conclusions

Multi-dose L-citrulline was well tolerated. These results will assist in the design of phase II RCTs evaluating L-citrulline dosage strategies to achieve target plasma L-citrulline concentrations in infants at risk for BPD-PH. Clinical trials.gov ID: NCT03542812
NTRK1 knockdown induces mouse cognitive impairment and hippocampal neuronal damage through mitophagy suppression via inactivating the AMPK/ULK1/FUNDC1 pathway
Authors: Yang Kai , Wu Jue , Li Shang , Wang Shan , Zhang Jing , Wang Yi-peng , Yan You-sheng , Hu Hua-ying , Xiong Ming-fang , Bai Chao-bo , Sun Yong-qing , Chen Wen-qi , Zeng Yang , Yuan Jun-liang , Yin Cheng-hong
Published: October 2023

Hippocampal neuronal damage may induce cognitive impairment. Neurotrophic tyrosine kinase receptor 1 (NTRK1) reportedly regulates neuronal damage, although the underlying mechanism remains unclear. The present study aimed to investigate the role of NTRK1 in mouse hippocampal neuronal damage and the specific mechanism. A mouse NTRK1-knockdown model was established and subjected to pre-treatment with BAY-3827, followed by a behavioral test, Nissl staining, and NeuN immunofluorescence (IF) staining to evaluate the cognitive impairment and hippocampal neuronal damage. Next, an in vitro analysis was conducted using the CCK-8 assay, TUNEL assay, NeuN IF staining, DCFH-DA staining, JC-1 staining, ATP content test, mRFP-eGFP-LC3 assay, and LC3-II IF staining to elucidate the effect of NTRK1 on mouse hippocampal neuronal activity, apoptosis, damage, mitochondrial function, and autophagy. Subsequently, rescue experiments were performed by subjecting the NTRK1-knockdown neurons to pre-treatment with O304 and Rapamycin. The AMPK/ULK1/FUNDC1 pathway activity and mitophagy were detected using western blotting (WB) analysis. Resultantly, in vivo analysis revealed that NTRK1 knockdown induced mouse cognitive impairment and hippocampal tissue damage, in addition to inactivating the AMPK/ULK1/FUNDC1 pathway activity and mitophagy in the hippocampal tissues of mice. The treatment with BAY-3827 exacerbated the mouse depressive-like behavior induced by NTRK1 knockdown. The results of in vitro analysis indicated that NTRK1 knockdown attenuated viability, NeuN expression, ATP production, mitochondrial membrane potential, and mitophagy, while enhancing apoptosis and ROS production in mouse hippocampal neurons. Conversely, pre-treatment with O304 and rapamycin abrogated the suppression of mitophagy and the promotion of neuronal damage induced upon NTRK1 silencing. Conclusively, NTRK1 knockdown induces mouse hippocampal neuronal damage through the suppression of mitophagy via inactivating the AMPK/ULK1/FUNDC1 pathway. This finding would provide insight leading to the development of novel strategies for the treatment of cognitive impairment induced due to hippocampal neuronal damage.
Adropin inhibits the progression of atherosclerosis in ApoE-/-/Enho-/- mice by regulating endothelial-to-mesenchymal transition
Authors: Ying Teng , Wu LingZhen , Lan TingXiang , Wei ZhiXiong , Hu DanQing , Ke YiLang , Jiang Qiong , Fang Jun
Published: October 2023

Adropin, a secreted protein, coded by energy homeostasis-associated gene (Enho), is recently reported to modulate atherogenesis, with endothelial-to-mesenchymal transition (EndMT) involved in the early process. We explored whether adropin may alleviate atherosclerosis by regulating EndMT. We found that an intraperitoneal injection of adropin [105 μg/(kg·d) for 13 weeks] inhibited the progression of high-fat diet (HFD)-induced aortic atherosclerosis in apolipoprotein E-deficient mice (ApoE–/–) and those with double gene deletion (ApoE–/–/Enho–/–), as detected by Oil Red O and haematoxylin-eosin staining. In the aortas of ApoE–/– mouse, adropin treatment ameliorated the decrease in the mRNA expression of endothelial cell markers (leukocyte differentiation antigen 31, CD31, and vascular endothelial cadherin, VE-cadherin), but increased that of EndMT markers (alpha smooth muscle actin, α-SMA, and fibroblasts specific protein-1). In vitro, an adropin treatment (30 ng/ml) arrested the hydrogen peroxide (H2O2)-induced EndMT in human umbilical vein endothelial cells (HUVECs), attenuated the morphological changes of HUVECs, reduced the number of immunofluorescence-positive α-SMA, increased the mRNA and protein expressions of CD31 and VE-cadherin, and decreased those of α-SMA. Furthermore, the adropin treatment decreased the mRNA and protein expressions of transforming growth factor (TGF)-β1 and TGF-β2, and suppressed the phosphorylation of downstream signal protein Smad2/3 in HUVECs. These mitigative effects of adropin on H2O2-induced EndMT were reversed by the transfection of TGF-β plasmid. The findings signify that adropin treatment may alleviate the atherosclerosis in ApoE–/–/Enho–/– mice by inhibiting EndMT via the TGF-β/Smad2/3 signaling pathway.
Heat shock protein DNAJA2 regulates transcription-coupled repair by triggering CSB degradation via chaperone-mediated autophagy
Authors: Huang Yaping , Gu Liya , Li Guo-Min
Published: October 2023

Transcription-coupled nucleotide excision repair (TC-NER) is an important genome maintenance system that preferentially removes DNA lesions on the transcribed strand of actively transcribed genes, including non-coding genes. TC-NER involves lesion recognition by the initiation complex consisting of RNA polymerase II (Pol II) and Cockayne syndrome group B (CSB), followed by NER-catalyzed lesion removal. However, the efficient lesion removal requires the initiation complex to yield the right of way to the excision machinery, and how this occurs in a timely manner is unknown. Here we show that heat shock protein DNAJA2 facilitates the HSC70 chaperone-mediated autophagy (CMA) to degrade CSB during TC-NER. DNAJA2 interacts with and enables HSC70 to recognize sumoylated CSB. This triggers the removal of both CSB and Pol II from the lesion site in a manner dependent on lysosome receptor LAMP2A. Defects in DNAJA2, HSC70 or LAMP2A abolish CSB degradation and block TC-NER. Our findings discover DNAJA2-mediated CMA as a critical regulator of TC-NER, implicating the DNAJA2-HSC70-CMA axis factors in genome maintenance.
HIV-1 diverts cortical actin for particle assembly and release
Authors: Dibsy Rayane , Bremaud Erwan , Mak Johnson , Favard Cyril , Muriaux Delphine
Published: October 2023

Enveloped viruses assemble and bud from the host cell membranes. Any role of cortical actin in these processes have often been a source of debate. Here, we assessed if cortical actin was involved in HIV-1 assembly in infected CD4 T lymphocytes. Our results show that preventing actin branching not only increases HIV-1 particle release but also the number of individual HIV-1 Gag assembly clusters at the T cell plasma membrane. Indeed, in infected T lymphocytes and in in vitro quantitative model systems, we show that HIV-1 Gag protein prefers areas deficient in F-actin for assembling. Finally, we found that the host factor Arpin, an inhibitor of Arp2/3 branched actin, is recruited at the membrane of infected T cells and it can associate with the viral Gag protein. Altogether, our data show that, for virus assembly and particle release, HIV-1 prefers low density of cortical actin and may favor local actin debranching by subverting Arpin.
Autonomous and dynamic precursor selection for solid-state materials synthesis
Authors: Szymanski Nathan J. , Nevatia Pragnay , Bartel Christopher J. , Zeng Yan , Ceder Gerbrand
Published: October 2023

Solid-state synthesis plays an important role in the development of new materials and technologies. While in situ characterization and ab-initio computations have advanced our understanding of materials synthesis, experiments targeting new compounds often still require many different precursors and conditions to be tested. Here we introduce an algorithm (ARROWS3) designed to automate the selection of optimal precursors for solid-state materials synthesis. This algorithm actively learns from experimental outcomes to determine which precursors lead to unfavorable reactions that form highly stable intermediates, preventing the target material’s formation. Based on this information, ARROWS3 proposes new experiments using precursors it predicts to avoid such intermediates, thereby retaining a larger thermodynamic driving force to form the target. We validate this approach on three experimental datasets, containing results from over 200 synthesis procedures. In comparison to black-box optimization, ARROWS3 identifies effective precursor sets for each target while requiring substantially fewer experimental iterations. These findings highlight the importance of domain knowledge in optimization algorithms for materials synthesis, which are critical for the development of fully autonomous research platforms.
Self-promoted electroactive biomimetic mineralized scaffolds for bacteria-infected bone regeneration
Authors: Li Zixin , He Danqing , Guo Bowen , Wang Zekun , Yu Huajie , Wang Yu , Jin Shanshan , Yu Min , Zhu Lisha , Chen Liyuan , Ding Chengye , Wu Xiaolan , Wu Tianhao , Gong Shiqiang , Mao Jing , Zhou Yanheng , Luo Dan , Liu Yan
Published: October 2023

Infected bone defects are a major challenge in orthopedic treatment. Native bone tissue possesses an endogenous electroactive interface that induces stem cell differentiation and inhibits bacterial adhesion and activity. However, traditional bone substitutes have difficulty in reconstructing the electrical environment of bone. In this study, we develop a self-promoted electroactive mineralized scaffold (sp-EMS) that generates weak currents via spontaneous electrochemical reactions to activate voltage-gated Ca2+channels, enhance adenosine triphosphate-induced actin remodeling, and ultimately achieve osteogenic differentiation of mesenchymal stem cells by activating the BMP2/Smad5 pathway. Furthermore, we show that the electroactive interface provided by the sp-EMS inhibits bacterial adhesion and activity via electrochemical products and concomitantly generated reactive oxygen species. We find that the osteogenic and antibacterial dual functions of the sp-EMS depend on its self-promoting electrical stimulation. We demonstrate that in vivo, the sp-EMS achieves complete or nearly complete in situ infected bone healing, from a rat calvarial defect model with single bacterial infection, to a rabbit open alveolar bone defect model and a beagle dog vertical bone defect model with the complex oral bacterial microenvironment. This translational study demonstrates that the electroactive bone graft presents a promising therapeutic platform for complex defect repair.
Eucalyptus torquata L. flowers: a comprehensive study reporting their metabolites profiling and anti-gouty arthritis potential
Authors: Ashour Rehab M. S. , El-Shiekh Riham A. , Sobeh Mansour , Abdelfattah Mohamed A. O. , Abdel-Aziz Marwa M. , Okba Mona M.
Published: October 2023

Gouty arthritis is one of the most common metabolic disorders affecting people. Plant based drugs can lower the risk of this health disorder. The anti-gouty potential of Eucalyptus torquata flowers methanol extract (ETME) was evaluated in vitro via measuring the inhibitory effects of five pro-inflammatory enzymes; xanthine oxidase (XO), hyaluronidase, lipoxygenase (5-LOX), cyclooxygenases COX-1, and COX-2, in addition to evaluating the inhibition of histamine release, albumin denaturation, membrane stabilization, tyrosinase, and protease inhibitory activities. Also, its antioxidant potential was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assays and ferric reducing power assay (FRAP). HPLC–PDA-MS/MS was used to identify the metabolites in the tested extract. The latter exhibited substantial anti-arthritic properties in all assays with comparable potential to the corresponding reference drugs. HPLC–MS/MS analysis of this bioactive extract tentatively annotated 46 metabolites including phloroglucinols, gallic and ellagic acids derivatives, terpenes, flavonoids, fatty acids, and miscellaneous metabolites. Our study highlights the medicinal importance of E. torquata as an anti-gouty candidate and opens new avenues of gouty management.
Ankle biomechanics of the three-step layup in a basketball player with chronic ankle instability
Authors: Wang Luyu , Ye Jiahui , Zhang Xuyang
Published: October 2023

At present, the effects of chronic ankle instability (CAI) on the biomechanics of the ankle joint in the three-step layup of basketball players are not clear. This work aims to thoroughly investigate the impact of CAI on the biomechanical characteristics of the ankle during the execution of a three-step layup in basketball players. Thirty male basketball players were stratified into distinct groups—namely, a CAI group and a non-CAI group—comprising 15 individuals each, based on the presence or absence of CAI. Demographic attributes, including age, weight, height, and the Cumberland Ankle Instability Tool (CAIT) score, were subjected to rigorous statistical examination within both athlete cohorts. The research employed four Whistler 9281CA 3D force measuring platforms (Switzerland), recording at 1000 Hz, in conjunction with eight camera motion analysis systems (USA), functioning at a frequency of 200 Hz. The study recorded maximal plantarflexion angle, inversion angle, dorsiflexion angle, and peak ankle dorsiflexion moment across the subjects during the distinct phases of push-off, landing, and the ensuing landing period. The findings notably exhibited that within the context of the one-foot push-off phase, the maximum ankle inversion angle was notably diminished in the CAI group as contrasted with the non-CAI group, demonstrating statistical significance (t = − 3.006, P < 0.01). The CAI group exhibited a lesser alteration in ankle inversion angle compared to the non-CAI group. Notably, during the one-foot landing period, the CAI group demonstrated a significantly greater maximum ankle inversion angle in contrast to the non-CAI group (t = 8.802, P < 0.001). Furthermore, the CAI group displayed a substantially larger maximum dorsiflexion angle at the ankle joint compared to the non-CAI group (t = 2.265, P < 0.05). Additionally, the CAI group exhibited a prolonged peak time for ankle dorsiflexion moment as compared to the non-CAI group (t = − 2.428, P < 0.05). Collectively, the findings elucidated a reduction in the maximum ankle joint inversion angle during the one-foot push-off phase in individuals with CAI. Furthermore, increased maximum inversion angle and maximum dorsiflexion angle of the ankle joint were observed during the one-foot landing period, alongside a lengthening of the peak time of ankle dorsiflexion moment. These results contribute valuable insights into the selection of training methodologies for basketball players afflicted by CAI.
Nano-silica from white silica sand functionalized with PANI-SDS (SiO2/PANI-SDS) as an adsorbent for the elimination of methylene blue from aqueous media
Authors: Salem Mohamed A. , Salem Ibrahim A. , El-Dahrawy Wafaa M. , El-Ghobashy Marwa A.
Published: October 2023

Natural resources including sand are one of the best approaches for treating dye-polluted wastewater. The SiO2/PANI-SDS nanocomposite was synthesized by self-assembly and intermolecular interaction. The physicochemical features of the SiO2/PANI-SDS nanocomposite were explored by FT-IR, XRD, SEM, TEM, EDX, and N2 adsorption–desorption techniques to be evaluated as an adsorbent for the MB. The surface area of the SiO2/PANI-SDS is 23.317 m2/g, the pore size is 0.036 cm3/g, and the pore radius is 1.91 nm. Batch kinetic studies at different initial adsorbate, adsorbent and NaCl concentrations, and temperatures showed excellent pseudo-second-order. Several isotherm models were applied to evaluate the MB adsorption on the SiO2/PANI-SDS nanocomposite. According to R2 values the isotherm models were fitted in the following order: Langmuir > Dubinin–Radushkevich (D–R) > Freundlich. The adsorption/desorption process showed good reusability of the SiO2/PANI-SDS nanocomposite.
A two-arm analysis of the immune response to heterologous boosting of inactivated SARS-CoV-2 vaccines
Authors: Nithichanon Arnone , Kamuthachad Ludthawun , Salao Kanin , Phoksawat Wisitsak , Kamsom Chatcharin , Wongratanacheewin Surasakdi , Pipattanaboon Chonlatip , Kanthawong Sakawrat , Yordpratum Umaporn , Aromseree Sirinart , Meesing Atibordee , Mootsikapun Piroon , Edwards Steven W. , Phanthanawiboon Supranee
Published: October 2023

Several vaccine programs were introduced during the COVID-19 pandemic, which included inactivated virus, DNA viral vectors and mRNA vaccines. Booster programs are recommended, especially for those in high-risk groups. However, many of these booster programs involve heterologous vaccines. This study enrolled volunteers who first received two full-dose CoronaVac vaccinations before receiving heterologous boosters with DNA- and/or mRNA-vaccines for an additional 2 doses (n = 40) or an additional 3 doses (n = 16). Our results showed no difference in side effects, neutralizing antibodies, or T-cell responses for any of the heterologous vaccination programs. However, the neutralizing capacity and IFN-γ responses against the Omicron variant in volunteers who received 4 or 5 doses were improved. Polarization of peripheral memory T cells after stimulation in all booster groups with Omicron peptide showed an increased trend of naïve and central memory phenotypes of both CD4+ and CD8+ T cells, suggesting that exposure to Omicron antigens will drive T cells into a lymphoid resident T cell phenotype. Our data support a continuous vaccination program to maximize the effectiveness of immunity, especially in people at high risk. Furthermore, the number of boosting doses is important for maintaining immunity.
Waterlogging increases greenhouse gas release and decreases yield in winter rapeseed (Brassica napus L.) seedlings
Authors: Li Linlin , Zhang Lang , Tang Jianwu , Xing Hucheng , Zhao Long , Jie Hongdong , Jie Yucheng
Published: October 2023

A sustainable future depends on increasing agricultural carbon (C) and nitrogen (N) sequestration. Winter rapeseeds are facing severe yield loss after waterlogging due to the effects of extreme rainfall, especially in the seedling stage, where rainfall is most sensitive. Uncertainty exists over the farming greenhouse gas (GHG) release of rapeseed seedlings following the onset of waterlogging. The effect of waterlogging on GHG release and leaf gas exchange in winter rapeseed was examined in a pot experiment. The experiment included waterlogging treatments lasting 7-day and 21-day and normal irrigation as a control treatment. According to our findings, (1) The ecosystem of rapeseed seedlings released methane (CH4) and nitrous oxide (N2O) in a clear up change that was impacted by ongoing waterlogging. Among them, N2O release had a transient rise during the early stages under the effect of seedling fertilizer. (2) The net photosynthetic rate, transpiration rate, stomatal conductance, plant height, soil moisture, and soil oxidation–reduction potential of rapeseed all significantly decreased due to the ongoing waterlogging. However, rapeseed leaves showed a significant increase in intercellular carbon dioxide (CO2) concentration and leaf chlorophyll content values after waterlogging. Additionally, the findings demonstrated an extremely significant increase in the sustained-flux global warming potential of the sum CO2-eq of CH4 and N2O throughout the entire waterlogging stress period. Therefore, continuous waterlogging can increase C and N release from rapeseed seedlings ecosystem and decrease yield. Therefore, we suggest increasing drainage techniques to decrease the release of agricultural GHGs and promote sustainable crop production.