bluuebunny/crossref_metadata_2025 huggingface dataset:
Dysregulation Serum miR-19a-3p is a Diagnostic Biomarker for Asymptomatic Carotid Artery Stenosis and a Promising Predictor of Cerebral Ischemia Events
Authors: Liu Xiaoliang , Zheng Xiaojun , Wang Ying , Liu Juan
Published: September 2021

This study aims to identify the diagnostic potential of microRNA-19a-3p (miR-19a-3p) for asymptomatic carotid artery stenosis (CAS) and clinical predictive potential for cerebral ischemia events (CIEs). Serum samples from 101 asymptomatic CAS patients and 98 healthy controls were collected. And it was found that serum miR-19a-3p in asymptomatic CAS patients was generally elevated ( P < .05). Increased miR-19a-3p in asymptomatic CAS was associated with severe CAS (odds ratio  = 3.920, 95% confidence interval [CI] = 1.482-10.372, P < .01). The area under the receiver operating characteristic (ROC) curve (AUC) was 0.905, indicating that the level of miR-19a-3p was statistically significant for the diagnosis of asymptomatic CAS. Furthermore, the level of serum miR-19a-3p (hazard ratio [HR] = 8.507, 95% confidence interval [CI] = 2.239-32.328, P = .002) and degree of artery stenosis (HR = 3.695, 95% CI = 1.127-12.109, P = .031) were independent predictors of occurrence of CIE. Moreover, patients with elevated miR-19a-3p levels were more likely to experience CIE than patients with low levels. Upregulated miR-19a-3p can be used as a diagnostic biomarker for asymptomatic CAS patients and as an independent predictor of CIE.
Chaotic motion control of a vibro-impact system based on data-driven control method
Authors: Wei Xiao-juan , Li Ning-zhou , Ding Wang-cai
Published: September 2021

For the chaotic motion control of a vibro-impact system with clearance, the parameter feedback chaos control strategy based on the data-driven control method is presented in this article. The pseudo-partial-derivative is estimated on-line by using the input/output data of the controlled system so that the compact form dynamic linearization (CFDL) data model of the controlled system can be established. And then, the chaos controller is designed based on the CFDL data model of the controlled system. And the distance between two adjacent points on the Poincaré section is used as the judgment basis to guide the controller to output a small perturbation to adjust the damping coefficient of the controlled system, so the chaotic motion can be controlled to a periodic motion by dynamically and slightly adjusting the damping coefficient of the controlled system. In this method, the design of the controller is independent of the order of the controlled system and the structure of the mathematical model. Only the input/output data of the controlled system can be used to complete the design of the controller. In the simulation experiment, the effectiveness and feasibility of the proposed control method in this article are verified by simulation results.
Child Abuse Pediatricians Assess a Low Likelihood of Abuse in Half of 2890 Physical Abuse Consults
Authors: Johnson Katie L. , Brown Emily C. B. , Feldman Kenneth W. , Qu Pingping , Lindberg Daniel M. ,   for the ExSTRA Investigators
Published: September 2021

The aim of this study was to examine the frequency with which child abuse pediatricians (CAPs) assess consultations as low versus high likelihood of abuse. In this retrospective secondary analysis of data from the Examining Siblings to Recognize Abuse (ExSTRA) study, the likelihood of abuse score for 2890 consultations at 20 medical centers was collected. Descriptive statistics were used to examine the percentage of cases representing low versus high likelihood of abuse (i.e., score of 1–4 vs. 5–7 on a 7-point scale). Linear and logistic regression analyses were used to examine score variability between medical centers. Overall, fifty-three percent of cases were assessed as low likelihood of abuse, suggesting that CAPs were equally as likely to assess a high versus low likelihood of abuse. The percentage of cases representing low likelihood of abuse differed significantly ( P < .001) between medical centers after controlling for patient age, sex, race/ethnicity, twin/triplet status, injury types, and injury severity. The variability between CAP assessments at different medical centers is discussed, along with potential contributors to this variability and directions for future work.
Inflammatory Mechanism and Clinical Implication of Asthma in COVID-19
Authors: Wisnu Wardana Vasa Adi , Rosyid Alfian Nur
Published: September 2021

Asthma is a chronic inflammatory disease of the respiratory tract that has become a public health problem in various countries. Referring to the Global Initiative for Asthma, the prevalence of asthma continues to increase especially in children. Coronavirus Disease 2019 (COVID-19) is an infectious disease caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) that has declared a pandemic by the world health organization on March 2020. For many years, it has been known that people with asthma have a worse impact on respiratory viral infections. Asthma has been listed by the centers for disease control and prevention as one of the risk factors for COVID-19, although several studies have different results. SARS-CoV-2 utilizes angiotensin-converting enzyme 2 (ACE2) as its cellular receptor, and it has been known that the expression of the ACE2 receptor is reduced in asthma patients. This reduced expression could also be accounted from the therapy of asthma. This paper aims to discuss the pathophysiology of asthma and COVID-19 and the susceptibility of asthma patients in contracting COVID-19.
Free nonlinear vibration analysis of nano-truncated conical shells based on modified strain gradient theory
Authors: Sheykhi Alireza , Hosseini-Hashemi Shahrokh , Maghsoudpour Adel , Haghighi Shahram Etemadi
Published: September 2021

In this study, the nonlinear free vibrations behaviour of nano-truncated conical shells was analysed, using the first-order shear deformable shell model. The analysis took into account the structure size through modified strain gradient theory, and differential quadrature and Fréchet derivative methods in von Kármán-Donnell-type approach to kinematic nonlinearity. The governing equations were obtained, utilizing Hamilton's principle. Partial differential equations plus the non-classical and classical boundary conditions were used to obtain the shells’ equations of motion. Discretizing the boundary conditions and equations of motion were performed based on a generalized differential quadrature analogy. The eigenvalue system was considered based on the harmonic balance technique. The Galerkin and Fréchet derivative approaches were used to determine the nonlinear free vibration behaviour of the carbon nano-cone, which was modelled in the simply- and clamped-supported boundary conditions. Comparisons were made between the findings from the new model versus the couple and classical stress theories, indicating that the classical and modified couple stress theories are distinct representations of modified strain gradient theory. The results also revealed that the degree of hardening of nano-truncated conical shells in the modified strain gradient theory is less than that of modified couple stress and classical theories. This led to a rise in the non-dimensional amplitude and frequency ratios. This study investigated the effect of size on free nonlinear vibrations of nano-truncated conical shells for various apex angles and lengths. Finally, we evaluated and compared our findings versus those reported by previous studies, which confirmed the precision and accuracy of our results.
Idiopathic orbital myositis in a pregnant woman
Authors: Wilson Caroline , Lai Jonathan , Morton Adam
Published: September 2021

A case of a 29-year-old woman who presented at 25 weeks’ gestation with acute onset of painful diplopia and periorbital swelling is presented. Following further investigation, a diagnosis of idiopathic acute lateral rectus myositis was established. Her condition resolved following a 4-week course of oral prednisolone without recurrence. A healthy female was delivered at 40 weeks’ gestation. The presenting features, differential diagnosis, treatment and course of orbital myositis are discussed.
High-intensity curvilinear movements’ relevance in semi-professional soccer: An approach from principal components analysis
Authors: Pino-Ortega José , Clemente Filipe Manuel , Palucci Vieira Luiz H , Rico-González Markel
Published: September 2021

Due to the high number of variables reported from tracking systems, the interest in data reduction techniques has grown. To date, principal component analysis (PCA) has been performed in soccer, but since the results depend on the variables included, a lack of objectivity continues to be of concern. The aim of this study was to highlight the variables that compose the principal components (PC) in semi-professional soccer, including all variables extracted from tracking systems. Data were collected from a semi-professional Spanish team that participated in 10 matches. From more than 250 variables, the PCA grouped a total of 19 variables in six PCs, explaining 72% of players’ external load. All variables were related to centripetal force, high intensity running, and high-intensity efforts and short efforts. Interestingly, the first PC was composed of four variables related to centripetal force. The current exploratory analysis indicated that, in addition to traditional high-intensity displacement variables, force measures should also be considered in soccer match analysis due to their effect on a player’s external load.
Frailty in inflammatory bowel diseases: an emerging concept
Authors: Kochar Bharati , Orkaby Ariela R. , Ananthakrishnan Ashwin N. , Ritchie Christine S.
Published: September 2021

Inflammatory bowel diseases (IBD), consisting of Crohn’s disease and ulcerative colitis, are chronic remitting, relapsing inflammatory conditions of the gastrointestinal tract. While traditionally a disease of younger ages, the number of older adults with IBD is rising rapidly. Patients with IBD often experience geriatric syndromes at earlier ages. Older adults with IBD have poorer disease and treatment-related outcomes compared with younger adults with IBD. Applying the principles of geriatrics to understanding a chronic disease in older adults may improve health span. Better tools are needed to stratify IBD patients who are at high risk for adverse events. Frailty is a geriatric construct that may approximate biologic age. Frailty is a complex, multi-dimensional syndrome that leads to increased vulnerability to stress and decline of reserve across multiple physiologic systems. In this review, we present the leading conceptual models of frailty and discuss the applications of frailty in immune-mediated diseases. We also review chronic conditions where frailty has been applied successfully as a tool for risk stratification. Finally, we discuss in the detail the growing body of literature highlighting the relationship between frailty and IBD, the epidemiology of frailty in IBD, and ramifications of frailty in IBD.
Perception of the Potential for Interaction in Social Scenes
Authors: Hessels Roy S. , Benjamins Jeroen S. , van Doorn Andrea J. , Koenderink Jan J. , Hooge Ignace T. C.
Published: September 2021

In urban environments, humans often encounter other people that may engage one in interaction. How do humans perceive such invitations to interact at a glance? We briefly presented participants with pictures of actors carrying out one of 11 behaviors (e.g., waving or looking at a phone) at four camera-actor distances. Participants were asked to describe what they might do in such a situation, how they decided, and what stood out most in the photograph. In addition, participants rated how likely they deemed interaction to take place. Participants formulated clear responses about how they might act. We show convincingly that what participants would do depended on the depicted behavior, but not the camera-actor distance. The likeliness to interact ratings depended both on the depicted behavior and the camera-actor distance. We conclude that humans perceive the “gist” of photographs and that various aspects of the actor, action, and context depicted in photographs are subjectively available at a glance. Our conclusions are discussed in the context of scene perception, social robotics, and intercultural differences.
Dealing with disappointment: How can a ‘coexisting imperatives’ view help us understand the unfulfilled dialogical promise of digital media
Authors: Shoai Andrés
Published: September 2021

The association between the concept of dialogue and the expansion of digital media in public relations started as a theoretical ‘promise’ and was later followed by a feeling of disappointment. This article argues that the dialogic promise of new media was in great measure a consequence of a well-established belief according to which the field was rapidly moving from a ‘functional’ to a ‘cocreational’ approach, whereas in fact both cocreational and functional imperatives persist and coexist in complex manners that need to be disentangled. This idea is explored through a critical analysis of highly cited literature about dialogue and digital technology in public relations. Implications for future theory-building, research and practice are discussed.
Preliminary Efficacy and Acceptability of an Online Exercise and Nutrition Workplace Wellness Program: A Brief Report
Authors: SantaBarbara Nicholas , Rezai Roxana , Terry Elisa , Shedd Kelly , Comulada W. Scott
Published: September 2021

Background

Workplace wellness programs (WWP) offer physiological and psychological benefits to employees and financial and productivity benefits to employers. However, the COVID-19 pandemic has prevented in-person sessions and has required WWP’s to transition to online platforms. The purpose of this brief report was to assess the preliminary feasibility, acceptability, and efficacy of a mobile version of the Bruin Health Improvement Program (BHIP mobile) WWP.

Methods

Participants virtually attended (i.e., via Zoom) twice weekly physical activity sessions and a once weekly nutrition seminar for 10-weeks with the option of a 30-minute one-on-one consultation with a registered dietician. Demographics, anthropometric indices, stress, muscular endurance, and aerobic fitness were assessed at baseline and follow-up. All analyses were conducted in SPSS v. 27.

Results

Twenty-seven participants (96% female) enrolled and 13 (100% female) completed the 10-week program. There were significant reductions in bodyweight ( p < .01) and body mass index ( p < .02) but not stress ( p > .05), and significant increases in muscular endurance ( p < .01) but not aerobic fitness ( p > .05). Overall, BHIP mobile appears to be acceptable to participants but logistical concerns such as inconsistent internet connection was noted as potential downfalls.

Conclusions/Applications to Practice

Improvements in health outcome among completers of a mobile WWP were promising. Completer feedback highlighted program strengths as the flexibility and comfort of attending exercise sessions and nutrition classes from home. Future versions of the program will allocate resources to improve completion and expand appeal for men and women.
Titanium Cervical Cage Subsidence: Postoperative Computed Tomography Analysis Defining Incidence and Associated Risk Factors
Authors: Pinter Zachariah W. , Reed Ryder , Townsley Sarah E. , Mikula Anthony L , Dittman Lauren , Xiong Ashley , Skjaerlund Jonathan , Michalopoulos Giorgos D. , Currier Bradford , Nassr Ahmad , Fogelson Jeremy L. , Freedman Brett A. , Bydon Mohamad , Kepler Christopher K. , Wagner Scott C , Elder Benjamin D. , Sebastian Arjun S.
Published: September 2021

Study Design

Retrospective cohort study

Objective

Substantial variability in both the measurement and classification of subsidence limits the strength of conclusions that can be drawn from previous studies. The purpose of this study was to precisely characterize patterns of cervical cage subsidence utilizing computed tomography (CT) scans, determine risk factors for cervical cage subsidence, and investigate the impact of subsidence on pseudarthrosis rates.

Methods

We performed a retrospective review of patients who underwent one- to three-levels of anterior cervical discectomy and fusion (ACDF) utilizing titanium interbodies with anterior plating between the years 2018 and 2020. Subsidence measurements were performed by two independent reviewers on CT scans obtained 6 months postoperatively. Subsidence was then classified as mild if subsidence into the inferior and superior endplate were both ≤2 mm, moderate if the worst subsidence into the inferior or superior endplate was between 2 to 4 mm, or severe if the worst subsidence into the inferior or superior endplate was ≥4 mm.

Results

A total of 51 patients (100 levels) were included in this study. A total of 48 levels demonstrated mild subsidence (≤2 mm), 38 demonstrated moderate subsidence (2-4 mm), and 14 demonstrated severe subsidence (≥4 mm). Risk factors for severe subsidence included male gender, multilevel constructs, greater mean vertebral height loss, increased cage height, lower Taillard index, and lower screw tip to vertebral body height ratio. Severe subsidence was not associated with an increased rate of pseudarthrosis.

Conclusion

Following ACDF with titanium cervical cages, subsidence is an anticipated postoperative occurrence and is not associated with an increased risk of pseudarthrosis.
The Unfinished Task of Christopher Columbus
Authors: Bousman Hugh
Published: September 2021

(This is an address presented at the Indonesia luncheon meeting of the South East Asia Committee, Division of Foreign Missions, National Council of the Churches of Christ in the U.S.A., on September 25, 1952. The Reverend Hugh Bousman is Associate General Secretary of the Philippine Federation of Christian Churches. While on furlough he is serving as an associate in the Far Eastern Joint Office. — Editor)
List of Theses in Foreign Missions and Related Subjects Accepted during the Academic Year 1955–1956
Authors: Person Laura
Published: September 2021

The compiler wishes to express deep gratitude to the professors of missions in 59 schools and seminaries for the reports which have made possible the compilation of the following list of dissertations and theses. No theses were written in 25 of the schools from which reports were received.

The annotations following the theses are by the professors who reported them. Neither compiler nor editor has examined these theses or passed judgment upon them. The professors making the reports have indicated that each particular thesis is related to foreign missions or, in a few instances, to home missions. It is assumed that such is the case where no annotation has been given.

Inquiries concerning a title should be addressed to the school concerned and not to the Missionary Research Library, An index to the theses will be found on paga 9.
Veterans’ Interpretation of Diabetes Distress in Diabetes Self-Management: Findings From Cognitive Interviews
Authors: Lewinski Allison A. , Shapiro Abigail , Bosworth Hayden B. , Crowley Matthew J. , McCant Felicia , Howard Teresa , Jeffreys Amy S. , McConnell Eleanor , Tanabe Paula , Barcinas Susan , Coffman Cynthia J. , King Heather A.
Published: September 2021

Purpose

The purpose of this project was to identify additional facets of diabetes distress (DD) in veterans that may be present due to the veteran’s military-related experience.

Methods

The study team completed cognitive interviews with veterans with type 2 diabetes mellitus (T2DM) to examine how they answered the Diabetes Distress Scale (DD Scale), a tool that assesses DD. The DD Scale was used because of its strong associations with self-management challenges, physician-related distress, and clinical outcomes.

Results

The veterans sample (n= 15) was 73% male, mean age of 61 (SD = 8.6), 53% Black, 53% with glycosylated hemoglobin level <9%, and 67% with prescribed insulin. The DD Scale is readily understood by veterans and interpreted. Thematic analysis indicated additional domains affecting DD and T2DM self-management, including access to care, comorbidities, disruptions in routine, fluctuations in emotions and behaviors, interactions with providers, lifelong nature of diabetes, mental health concerns, military as culture, personal characteristics, physical limitations, physical pain, sources of information and support, spirituality, and stigma.

Conclusions

This study describes how a veteran’s military experience may contribute to DD in the context of T2DM self-management. Findings indicate clinicians and researchers should account for additional domains when developing self-management interventions and discussing self-management behaviors with individuals with T2DM.
A novel 3D culture model recapitulates primary FL B-cell features and promotes their survival
Authors: Lamaison Claire , Latour Simon , Hélaine Nelson , Le Morvan Valérie , Saint-Vanne Julien , Mahouche Isabelle , Monvoisin Céline , Dussert Christelle , Andrique Laëtitia , Deleurme Laurent , Dessauge Elise , Pangault Céline , Baulande Sylvain , Legoix Patricia , Seffals Marine , Broca-Brisson Léa , Alessandri Kévin , Carlotti Martina , Soubeyran Pierre , Merlio Jean-Philippe , Mourcin Frédéric , Nassoy Pierre , Recher Gaëlle , Tarte Karin , Bresson-Bepoldin Laurence
Published: September 2021

Non-Hodgkin B-cell lymphomas (B-NHL) mainly develop within lymph nodes as aggregates of tumor cells densely packed with their surrounding microenvironment, creating a tumor niche specific to each lymphoma subtypes. In vitro preclinical models mimicking biomechanical forces, cellular microenvironment, and 3D organization of B-cell lymphomas remain scarce, while all these parameters are key determinants of lymphomagenesis and drug resistance. Using a microfluidic method based on cell encapsulation inside permeable, elastic, and hollow alginate microspheres, we developed a new tunable 3D model incorporating lymphoma B cells, extracellular matrix (ECM), and/or tonsil stromal cells (TSC). Under 3D confinement, lymphoma B cells were able to form cohesive spheroids resulting from overexpression of ECM components. Moreover, lymphoma B cells and TSC dynamically formed self-organized 3D spheroids favoring tumor cell growth. 3D culture induced resistance to the classical chemotherapeutic agent doxorubicin, but not to the BCL2 inhibitor ABT-199, identifying this approach as a relevant in vitro model to assess the activity of therapeutic agents in B-NHL. RNA-sequence analysis highlighted the synergy of 3D, ECM, and TSC in upregulating similar pathways in malignant B cells in vitro than those overexpressed in primary lymphoma B cells in situ. Finally, our 3D model including ECM and TSC allowed long-term in vitro survival of primary follicular lymphoma B cells. In conclusion, we propose a new high-throughput 3D model mimicking lymphoma tumor niche and making it possible to study the dynamic relationship between lymphoma B cells and their microenvironment and to screen new anti-cancer drugs.
Anti–IL-6 cytokine treatment has no impact on elevated hematocrit or splenomegaly in a polycythemia vera mouse model
Authors: Baldauf Conny K. , Müller Peter , Haage Tobias R. , Adam-Frey Stephanie , Lokau Juliane , Garbers Christoph , Fischer Thomas
Published: September 2021

Somatic mutations in JAK2, MPL and Calreticulin and inflammation play a key role in pathophysiology of chronic myeloproliferative neoplasia (CMN). One of the most prominent cytokines elevated in serum of Polycythemia vera patients is interleukin-6 (IL-6). Currently, it is being discussed whether suppression of inflammation by anti-cytokine approaches as anti-IL-6 treatment may be therapeutically useful in CMN. We here sought to investigate the efficacy of anti-IL-6 treatment on inflammatory cytokines, hematocrit and splenomegaly in CMN like disease. JAK2-V617F knock-in mice (JAK2+/V617F) were treated for three weeks with anti-IL-6 antibody (Ab) or IgG-control. Upon anti-IL-6 Ab treatment, serum levels of CXCL2 and CXCL10 were significantly reduced. In addition, CXCL1, CCL11, M-CSF, G-CSF, IL-17, IL-12p40 and CCL2 were reduced by a factor of 0.3 -- 0.8. Partly, this was also achieved by applying high-dose IgG. Hematocrit, erythrocyte and leukocyte counts were elevated in JAK2+/V617F mice but were not reduced by anti-IL6 Ab treatment. In addition, there was no apparent amelioration of splenomegaly and spleen histopathology. In conclusion, anti-IL-6 Ab treatment did not result in improvement of hematological disease parameters but was shown to modulate the serum cytokine signature.
Defining the AHR-regulated transcriptome in NK cells reveals gene expression programs relevant to development and function
Authors: Trikha Prashant , Moseman Jena E. , Thakkar Aarohi , Campbell Amanda R. , Elmas Ezgi , Foltz Jennifer A. , Chakravarti Nitin , Fitch James R. , Mardis Elaine R. , Lee Dean A.
Published: September 2021

The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that regulates cellular processes in cancer and immunity, including innate immune cell development and effector function. However, the transcriptional repertoire through which AHR mediates these effects remains largely unexplored. To elucidate the transcriptional elements directly regulated by AHR in natural killer (NK) cells, we performed RNA and chromatin immunoprecipitation sequencing on NK cells exposed to AHR agonist or antagonist. We show that mature peripheral blood NK cells lack AHR, but its expression is induced by Stat3 during interleukin-21–driven activation and proliferation, coincident with increased NCAM1 (CD56) expression resulting in a CD56bright phenotype. Compared with control conditions, NK cells expanded in the presence of the AHR antagonist, StemRegenin-1, were unaffected in proliferation or cytotoxicity, had no increase in NCAM1 transcription, and maintained the CD56dim phenotype. However, it showed altered expression of 1004 genes including those strongly associated with signaling pathways. In contrast, NK cells expanded in the presence of the AHR agonist, kynurenine, showed decreased cytotoxicity and altered expression of 97 genes including those strongly associated with oxidative stress and cellular metabolism. By overlaying these differentially expressed genes with AHR chromatin binding, we identified 160 genes directly regulated by AHR, including hallmark AHR targets AHRR and CYP1B1 and known regulators of phenotype, development, metabolism, and function such as NCAM1, KIT, NQO1, and TXN. In summary, we define the AHR transcriptome in NK cells, propose a model of AHR and Stat3 coregulation, and identify potential pathways that may be targeted to overcome AHR-mediated immune suppression.
The binding of autotaxin to integrins mediates hyperhomocysteinemia-potentiated platelet activation and thrombosis in mice and humans
Authors: Han Lulu , Miao Yutong , Zhao Yang , Zhang Xingzhong , Ma Xiaolong , Du Xing , Kong Wei , Xu Qingbo , Liu Junling , Dai Kesheng , Feng Juan , Wang Xian
Published: September 2021

Hyperhomocysteinemia (HHcy) is associated with an exaggerated platelet thrombotic response at sites of vascular injury. In this study, human medical examination showed that elevated human plasma Hcy levels correlated positively with enhanced blood coagulation and platelet activity, suggesting that humans with HHcy are more prone to thrombus formation at the sites of vascular injury. Accordingly, we observed accelerated platelet activation, primary hemostasis, and thrombus formation in apolipoprotein E-deficient (ApoE−/−) mice with acute or chronic HHcy. Upon homocysteine (Hcy) administration in C57BL/6J mice, platelet aggregation, spreading and clot retraction were markedly induced. More important, Hcy increased the affinity of platelet integrin αIIbβ3 with ligands and enhanced integrin outside-in signaling by promoting membrane phosphatidylserine exposure in vitro. Mechanistically, lipidomics analysis showed that lysophosphatidylcholines were the primary metabolites leading to clustering of HHcy-stimulated platelets. Cytosolic phospholipase A2 (cPLA2) activity and autotaxin (ATX, a secreted lysophospholipase D) secretion were upregulated by Hcy, leading to membrane phospholipid hydrolysis and PS exposure. Moreover, secreted ATX directly interacted with integrin β3. Inhibitors of cPLA2 and ATX activity blocked integrin αIIbβ3 outside-in signaling and thrombosis in HHcy ApoE−/− mice. In this study, we identified a novel mechanism by which HHcy promotes platelet membrane phospholipid catabolism and extracellular ATX secretion to activate integrin outside-in signaling, consequently exacerbating thrombosis and the results revealed an innovative approach to treating HHcy-related thrombotic diseases.
Calcitonin receptor-like (CALCRL) is a marker of stemness and an independent predictor of outcome in pediatric AML
Authors: Angenendt Linus , Wöste Marius , Mikesch Jan-Henrik , Arteaga Maria Francisca , Angenendt Adrian , Sandmann Sarah , Berdel Wolfgang E. , Lenz Georg , Dugas Martin , Meshinchi Soheil , Schliemann Christoph , Rössig Claudia
Published: September 2021

We have recently identified the G protein-coupled neuropeptide receptor calcitonin receptor-like (CALCRL) as an independent prognostic biomarker and a therapeutic target in more than 1500 adult patients with acute myeloid leukemia (AML). Here, we confirmed CALCRL expression as a prognostic factor in a cohort of 284 pediatric patients with AML. High CALCRL expression was independently associated with event-free survival (hazard ratio [HR], 1.87; 95% confidence interval [CI], 1.36-2.57; P = .0001), overall survival (HR, 1.55; 95% CI, 1.06-2.27; P = .025), and cumulative incidence of relapse (HR, 2.10; 95% CI, 1.49-1.96; P < .0001) when adjusting for age, white blood cell count, and genetic risk. Despite its association with leukemia stem cell signatures, CALCRL expression remained associated with all end points when compared with the 17-gene leukemic stem cell score. The strong association of CALCRL expression with the risk of relapse also in the pediatric population supports its role as novel age-independent master regulator of relapse-initiating, drug-tolerant AML cells in humans.
Clinical impact of NPM1-mutant molecular persistence after chemotherapy for acute myeloid leukemia
Authors: Tiong Ing S. , Dillon Richard , Ivey Adam , Kuzich James A. , Thiagarajah Nisha , Sharplin Kirsty M. , Kok Chung Hoow , Tedjaseputra Aditya , Rowland James P. , Grove Carolyn S. , Abro Emad , Shortt Jake , Hiwase Devendra K. , Bajel Ashish , Potter Nicola E. , Smith Matthew L. , Hemmaway Claire J. , Thomas Abin , Gilkes Amanda F. , Russell Nigel H. , Wei Andrew H.
Published: September 2021

Monitoring of NPM1 mutant (NPM1mut) measurable residual disease (MRD) in acute myeloid leukemia (AML) has an established role in patients who are treated with intensive chemotherapy. The European LeukemiaNet has defined molecular persistence at low copy number (MP-LCN) as an MRD transcript level <1% to 2% with a <1-log change between any 2 positive samples collected after the end of treatment (EOT). Because the clinical impact of MP-LCN is unknown, we sought to characterize outcomes in patients with persistent NPM1mut MRD after EOT and identify factors associated with disease progression. Consecutive patients with newly diagnosed NPM1mut AML who received ≥2 cycles of intensive chemotherapy were included if bone marrow was NPM1mut MRD positive at the EOT, and they were not transplanted in first complete remission. One hundred patients were followed for a median of 23.5 months; 42% remained free of progression at 1 year, either spontaneously achieving complete molecular remission (CRMRD−; 30%) or retaining a low-level NPM1mut transcript (12% for ≥12 months and 9% at last follow-up). Forty percent met the criteria for MP-LCN. Preemptive salvage therapy significantly prolonged relapse-free survival. Risk factors associated with disease progression were concurrent FLT3-internal tandem duplication at diagnosis and suboptimal MRD response (NPM1mut reduction <4.4-log) at EOT.
The antimicrobial peptide S100A8/A9 produced by airway epithelium functions as a potent and direct regulator of macrophage phenotype and function
Authors: Skronska-Wasek Wioletta , Durlanik Sibel , Le Huy Quang , Schroeder Victoria , Kitt Kerstin , Garnett James Peter , Pflanz Stefan
Published: September 2021

Background

Elevated counts of alveolar macrophages and attenuated phagocytic capacity are associated with chronic obstructive pulmonary disease (COPD). Factors governing macrophage phagocytosis are poorly understood. In this study we aimed to compare the influence of airway epithelial cell secretions from individuals with COPD and without COPD (non-COPD) on macrophage phagocytic activity, and the role of antimicrobial peptides (AMPs).

Methods

Supernatants from non-COPD and COPD small airway epithelial cell (SAEC) cultures exposed to non-typeableHaemophilus influenzae(NTHi) were applied to human monocyte-derived macrophages (MDMs) to assess their influence on phagocytosis. SAECs were analysed for changes in AMP expression by quantitative reverse transcription PCR, and the influence of select AMPs on macrophage phenotype and function was assessed by flow cytometry and metabolic activity assay.

Results

Secretions from the apical and basolateral surface of NTHi-exposed SAECs from non-COPD donors elicited superior phagocytic capacity in MDMs. Moreover, NTHi exposure led to a rapid increase in the expression of a range of AMPs by non-COPD SAECs, but this response was delayed in COPD SAECs. We demonstrate that treatment with AMPs β-defensin 2 and S100 calcium binding protein A8/S100 calcium binding protein A9 (S100A8/A9) improved the phagocytic capacity of MDMs. In-depth analysis of the influence of S100A8/A9 on MDMs revealed a role for this AMP in macrophage phenotype and function. Furthermore, we show that the expression of S100A8 and S100A9 is directly regulated by WNT/β-catenin signalling, a known deregulated pathway in COPD.

Conclusion

In conclusion, for the first time, we demonstrate that airway epithelium from patients with COPD has a reduced capacity to support the phagocytic function of macrophages in response to acute NTHi exposure, and we identify the WNT/β-catenin signalling-modulated and epithelium-derived S100A8/A9 as a potent regulator of macrophage phenotype and function.
Quantification of aerosol dispersal from suspected aerosol-generating procedures
Authors: Strand-Amundsen Runar , Tronstad Christian , Elvebakk Ole , Martinsen Tormod , Dybwad Marius , Lingaas Egil , Tønnessen Tor Inge
Published: September 2021

Background

Oxygen-delivering modalities like humidified high-flow nasal cannula (HFNC) and noninvasive positive-pressure ventilation (NIV) are suspected of generating aerosols that may contribute to transmission of disease such as coronavirus disease 2019. We sought to assess if these modalities lead to increased aerosol dispersal compared to the use of non-humidified low-flow nasal cannula oxygen treatment (LFNC).

Methods

Aerosol dispersal from 20 healthy volunteers using HFNC, LFNC and NIV oxygen treatment was measured in a controlled chamber. We investigated effects related to coughing and using a surgical face mask in combination with the oxygen delivering modalities. An aerodynamic particle sizer measured aerosol particles (APS3321, 0.3–20 µm) directly in front of the subjects, while a mesh of smaller particle sensors (SPS30, 0.3–10 µm) was distributed in the test chamber.

Results

Non-productive coughing led to significant increases in particle dispersal close to the face when using LFNC and HFNC but not when using NIV. HFNC or NIV did not lead to a statistically significant increase in aerosol dispersal compared to LFNC. With non-productive cough in a room without air changes, there was a significant drop in particle levels between 100 cm and 180 cm from the subjects.

Conclusions

Our results indicate that using HFNC and NIV does not lead to increased aerosol dispersal compared to low-flow oxygen treatment, except in rare cases. For a subject with non-productive cough, NIV with double-limb circuit and non-vented mask may be a favourable choice to reduce the risk for aerosol spread.
Associations of childhood unintentional injuries with maternal emotional status during COVID-19
Authors: Guo Xiangrong , Hua Hui , Xu Jian , Liu Zhiwei
Published: September 2021

Background

To explore the characteristics of unintentional childhood-injury during the COVID-19 pandemic and assess the association of unintentional-injury with maternal emotional status.

Methods

A cross-sectional survey was conducted with a convenience sample of 1300 children under 12-years-old from 21 schools (including nurseries/ kindergartens/ primary schools) in Wuhan and Shanghai during March to April 2020, and the mothers completed questionnaires online. Self-rating Depression/Anxiety Scales were used to evaluate maternal emotional status, questions on child unintentional-injury were based on the International-Statistical-Classification-of-Diseases-and-Related-Health-Problems-version-10 (ICD-10), and a total of 11 kinds of unintentional injuries were inquired. Information on socio-demographic and family-background factors was also collected.

Results

The children of 0–4, 5–9, and 10–12 years accounted for 29.2, 55.2 and 15.6%, respectively, the unintentional-injury rates were 10.29, 4.18 and 3.45%, respectively (P < 0.001), and boys had higher rates than girls. The three leading causes included “being struck by/against”, falls and animal bites (traffic-injury accounted for a small proportion). Lower maternal educational, living in suburban/rural (vs. urban) areas, grandparents (vs. mothers) being main caregivers, more child exposure to secondhand smoke, close relatives being suspected/ confirmed COVID-19 cases were associated with a higher risk of child unintentional-injury. After adjusting for related confounders, higher maternal depression levels were associated with a higher risk of unintentional injury.

Conclusions

The characteristics of unintentional childhood injury were different from those in non-pandemic periods. The main causes, risk factors and the association of unintentional injury with maternal depression deserve attention for development of effective measures for preventing children from unintentional injury during COVID-19 pandemic.

Graphical abstract
Test@work: evaluation of workplace HIV testing for construction workers using the RE-AIM framework
Authors: Jones Wendy , Somerset Sarah , Evans Catrin , Whittingham Katharine , Middleton Matthew , Blake Holly
Published: September 2021

Background

Community testing for HIV can reach previously untested populations but is rarely offered in workplaces. Targeting the construction sector could reach workers from high risk populations.

Methods

The RE-AIM framework was used to evaluate Test@Work, a workplace HIV testing intervention for construction workers implemented at 21 events (10 companies) in the UK.

Test@Work had three components: 1) an online health toolkit to inform managers about health screening and HIV testing; 2) general health checks; and 3) opt-in HIV consultation and testing. Quantitative data were collected using registration and exit questionnaires with workers (n = 426) and pre/post-event questionnaires with managers (n = 15), with qualitative analysis of free text responses.

Results

Reach 426 individuals had health checks. Participants were broadly representative of the UK construction workforce, but with a higher proportion of permanent workers. Most workers reported being in good health but also believed their work had an adverse impact on their health. Effectiveness: 97% of health check participants opted to have a consultation about sexual health (n = 413) and 82% had an HIV test (n = 348), of whom 78% had not previously been tested. All HIV tests were non-reactive. HIV testing at work was considered acceptable by most participants. Participants reported learning new things about their health (74%), said they would make changes as a result (70%) and felt confident of success (median score 8/10). Adoption: Recruitment of companies was challenging and time consuming. Seven of the participating companies were very large, employing over 1000 workers, which is atypical of construction generally. Implementation: All events were completed as planned and were considered successful by all parties. Maintenance: All managers would arrange further events if they were offered them. Six managers incorporated sexual health awareness into their health programmes, but this was not possible for many as health agendas were set centrally by their organisations.

Conclusions

Opt-in HIV testing, when embedded within a general health check, has high uptake and acceptability in the UK construction sector, and reaches individuals at risk for HIV who may not otherwise attend for testing. Cost-effectiveness of this approach is yet to be determined.

Trial registration

ClinicalTrials.gov Identifier: NCT04292002.
A community-engaged approach to understanding environmental health concerns and solutions in urban and rural communities
Authors: Wang Suwei , Richardson Molly B. , Evans Mary B. , Johnson Ethel , Threadgill-Matthews Sheryl , Tyson Sheila , White Katherine L. , Gohlke Julia M.
Published: September 2021

Background

Focus groups and workshops can be used to gain insights into the persistence of and potential solutions for environmental health priorities in underserved areas. The objective of this study was to characterize focus group and workshop outcomes of a community-academic partnership focused on addressing environmental health priorities in an urban and a rural location in Alabama between 2012 and 2019.

Methods

Six focus groups were conducted in 2016 with 60 participants from the City of Birmingham (urban) and 51 participants from Wilcox County (rural), Alabama to discuss solutions for identified environmental health priorities based on previous focus group results in 2012. Recorded focus groups were transcribed and analyzed using the grounded theory approach. Four follow-up workshops that included written survey instruments were conducted to further explore identified priorities and determine whether the priorities change over time in the same urban (68 participants) and rural (72 participants) locations in 2018 and 2019.

Results

Consistent with focus groups in 2012, all six focus groups in 2016 in Birmingham identified abandoned houses as the primary environmental priority. Four groups listed attending city council meetings, contacting government agencies and reporting issues as individual-level solutions. Identified city-level solutions included city-led confiscation, tearing down and transferring of abandoned property ownership. In Wilcox County, all six groups agreed the top priority was drinking water quality, consistent with results in 2012. While the priority was different in Birmingham versus Wilcox County, the top identified reason for problem persistence was similar, namely unresponsive authorities. Additionally, individual-level solutions identified by Wilcox County focus groups were similar to Birmingham, including contacting and pressuring agencies and developing petitions and protesting to raise awareness, while local policy-level solutions identified in Wilcox County included government-led provision of grants to improve septic systems, and transparency in allocation of funds. Workshops in 2018 and 2019 further emphasized water quality as the top priority in Wilcox County, while participants in Birmingham transitioned from abandoned houses as a top priority in 2018 to drinking water quality as a new priority in 2019.

Conclusions

Applying a community-engaged approach in both urban and rural locations provided better understanding of the unique opportunities and challenges for identifying potential interventions for environmental health priorities in both locations. Results can help inform future efforts to address locally defined environmental health issues and solutions.
Anomalous insertion of anterior and posterior horns of medial meniscus. Case report
Authors: Mariani Pier Paolo , Battaglia Michael J , Torre Guglielmo
Published: September 2021

Background

Anatomical variations of the attachment of medial meniscus are a common finding. However, anomalies of the posterior horn are extremely rare. Only two cases of posterior root anomaly have been described prior to the routine use of arthroscopy for evaluation and treatment of meniscal pathology. In this report, we present an anomaly of both the anterior and posterior roots of the medial meniscus that posed both a diagnostic and therapeutic dilemma.

Case presentation

The patient is young male soccer player who is currently 16 years of age and began having the atraumatic onset of pain and symptoms that limited performance starting at age 14 and was referred for failure of response to nonoperative treatment. Diagnostic arthroscopy revealed the presence of an anteromedial meniscofemoral ligament whereas the posterior root showed no bony attachment. The radiographic and arthroscopic findings are described. The clinical decision was made after to proceed with observation, reassurance, and gradual return to full activity with physiotherapy guidance.

Discussion and conclusion

The absence of injury, the mild complaints reported by the patient, his age, skeletal immaturity, and remaining growth led us to adopt a conservative approach to treating this anatomic variant and currently the patient is able to participate fully in sports without symptoms or restrictions.
Society, family, and individual characteristics as double-edged swords in the social reintegration of Iranian female survivors from unintentional severe burns: a qualitative study of enablers and barriers
Authors: Hemmati Maslakpak Masumeh , Ajoudani Fardin , Lotfi Mojgan , Alinejad Vahid
Published: September 2021

Background

Reintegrating to society is a significant challenge during burn survivors’ rehabilitation.

Aim

This study aims to describe what Iranian female survivors from unintentional severe burns experience as enablers and barriers of social reintegration (SR).

Methods

Fourteen adult female burn survivors whose burns were unintentional participated in this qualitative study. Data were gathered through semi-structured face-to-face or telephone interviews and analyzed using inductive content analysis.

Results

Thirteen subcategories and six categories were emerged. Categories and subcategories of enablers content area were as follows: positive impact of society on SR (normal treatment of society, instrumental support), positive impact of family on SR (magnifying personal abilities assets, empathy and emotional support), and positive impact of personal characteristics on SR (coping with others stares, right to have a normal social life). Categories and subcategories of Barriers content area were as follows: negative impact of society on SR (being questioned in public, incorrect judgment about intent of burns, burns as a contagious disease), negative impact of family on SR (embarrassment of appearing in public with the survivor, family mistrust), and negative impact of intra-personal factors on SR (exaggeration of the post-burn changes, being over-sensitive to the others looks). From the deep interpretation of the data two overarching themes were emerged: “acceptance of the new normal by the society and the individual” and “being encompassed by misconceptions and mistreatments”.

Conclusions

Society, family and the individual characteristics have a dual role to play in the success of social reintegration in Iranian female unintentional burn survivors.