bluuebunny/crossref_metadata_2025 huggingface dataset:
The Impact of Hyperbaric Oxygen Therapy on Serum C-Reactive Protein Levels, Osteoprotegerin Expression, and Osteoclast Numbers in Induced-Periodontitis Diabetic Rats
Authors: Mulawarmanti Dian , Parisihni Kristanti , Widyastuti Widyastuti
Published: May 2020

Objectives This study aimed to examine the impact of hyperbaric oxygen therapy (HBOT) on serum C-reactive protein (CRP) levels, osteoclast numbers, and osteoprotegerin (OPG) expression in periodontitis-induced diabetic rats

Materials and Methods This study constituted an in vivo laboratory-based experiment incorporating a posttest only control group design. Thirty male Wistar rats were divided into three groups of research subjects: a healthy group (K0), periodontitis-induced diabetic group (K1), and periodontitis-induced diabetic group treated with HBOT for 7 days (K2). After treatment, the subjects were sacrificed to determine the level of serum CRP by the ELISA method. Immunohistochemical analysis was conducted to check the level of OPG expression, while a histological analysis was undertaken to quantify the number of osteoclasts.

Statistical Analysis The data was analyzed using a one-way ANOVA and Least Significant Difference (LSD) test on which a result of p < 0.05 was considered statistically significant.

Results HBOT appreciably decreased serum CRP levels, significantly enhancing OPG expression in periodontitis-induced diabetic (p < 0.05) and decreasing the number of osteoclasts in -periodontitis-induced diabetic (p > 0.05).

Conclusion HBOT reduced the serum CRP level, increased OPG expression, and decreased osteoclast numbers in periodontitis-induced diabetic rats.
Conservation value of a subtropical reef in south-eastern Queensland, Australia, highlighted by citizen-science efforts
Authors: Grol Monique G. G. , Vercelloni Julie , Kenyon Tania M. , Bayraktarov Elisa , van den Berg Cedric P. , Harris Daniel , Loder Jennifer A. , Mihaljevic Morana , Rowland Phebe I. , Roelfsema Chris M.
Published: May 2020

Subtropical reefs are important habitats for many marine species and for tourism and recreation. Yet, subtropical reefs are understudied, and detailed habitat maps are seldom available. Citizen science can help fill this gap, while fostering community engagement and education. In this study, 44 trained volunteers conducted an ecological assessment of subtropical Flinders Reef using established Reef Check and CoralWatch protocols. In 2017, 10 sites were monitored to provide comprehensive information on reef communities and to estimate potential local drivers of coral community structure. A detailed habitat map was produced by integrating underwater photos, depth measurements, wave-exposure modelling and satellite imagery. Surveys showed that coral cover ranged from 14% to 67%. Site location and wave exposure explained 47% and 16% respectively, of the variability in coral community composition. Butterflyfishes were the most abundant fish group, with few invertebrates being observed during the surveys. Reef impacts were three times lower than on other nearby subtropical reefs. These findings can be used to provide local information to spatial management and Marine Park planning. To increase the conservation benefits and to maintain the health of Flinders Reef, we recommend expanding the current protection zone from 500- to a 1000-m radius.
Effect ofkojistarter on metabolites in Japanese alcoholic beverage sake made from the sake riceKoshitanrei
Authors: Ichikawa Eri , Hirata Shougo , Hata Yuko , Yazawa Hisashi , Tamura Hiroyasu , Kaneoke Mitsuoki , Iwashita Kazuhiro , Hirata Dai
Published: May 2020

In sake brewing, the steamed rice is used in 2 ways, added to sake-mash and making rice-koji. Rice-koji is made from the steamed rice by using koji starter, and its quality is an important determinant of the aroma/taste of sake. The sake rice Koshitanrei (KOS) was developed in Niigata Prefecture by crossing 2 sake rice varieties, Gohyakumangoku and Yamadanishiki. Recently, we reported the characteristic components/metabolites in sake made from KOS by conducting metabolome analysis using UPLC-QTOF-MS. In this study, to investigate the effect of koji starter and sake rice cultivars on the sake metabolites, we performed small-scale sake-making tests using the above 3 rice cultivars and 3 koji starters. Finally, we demonstrated that some of the characteristic components/metabolites of sake from KOS are affected by the koji starter. Thus, in addition to rice cultivar, koji starter plays an important role for establishment/maintenance of the quality of the final product.
Anti-inflammatory action of herbal medicine comprised ofScutellaria baicalensisandChrysanthemum morifolium
Authors: Suh Min Geun , Choi Hyeon-Son , Cho Kyoungwon , Park Sung Sun , Kim Woo Jung , Suh Hyung Joo , Kim Hoon
Published: May 2020

Various mixtures were prepared depending on the mixing ratio of Scutellaria baicalensis hot water extract (SB-HW), and Chrysanthemum morifolium ethanol extract (CM-E) and their anti-inflammatory activity were compared. Among them, SB-HW (80 μg/mL)/CM-E (120 μg/mL) or SB-HW (40 μg/mL)/CM-E (160 μg/mL) significantly inhibited LPS-stimulated NO and IL-6 levels in RAW 264.7 cells. The SB-HW (80 μg/mL)/CM-E (120 μg/mL) mixture, which was determined as active mixture, significantly reduced MUC5AC secretion in PMA and LPS-induced NCI-H292 cells. The active mixture also reduced the production of PGE2 and IL-8 in PMA-induced A549 cells. LC-MS/MS analysis showed that the active mixture was composed of high contents of flavone glycosides, such as baicalin and cynaroside. Western blot analysis indicated that the active mixture suppressed phosphorylation of ERK, JNK, and p38, associating with the inhibition of MAPK signaling. Taken together, our results suggest that the active mixture could be applied as a new anti-inflammatory herbal medicine.

Abbreviations

JNK: c-Jun N-terminal kinases; COPD: chronic obstructive pulmonary disease; CM: Chrysanthemum morifolium; COX-2: cyclooxygenase-2; ERK: extracellular-signal-regulated kinase; IL-6: interleukin-6; IL-8: interleukin-8; IL-12: interleukin-12; LPS: lipopolysaccharide; MAPK: mitogen-activated protein kinase; NO: nitric oxide; NK- κB: nuclear factor kappa B; p38: p38 mitogen-activated protein kinases; PBS: phosphate buffered saline; PMA: phorbol-12-myristate-13-acetate; SB: Scutellaria baicalensis; PGE2: prostaglandin E2; TBST: Tris-buffered saline containing 0.1% Tween 20; TIC: total ion chromatogram; TNF-α: tumor necrosis factor-alpha
Influence of freeze-drying and spray-drying preservation methods on survivability rate of different types of protectants encapsulated Lactobacillus acidophilus FTDC 3081
Authors: Tang Hock Wei , Abbasiliasi Sahar , Murugan Paramasivam , Tam Yew Joon , Ng Hui Suan , Tan Joo Shun
Published: May 2020

The aims of this study were to compare the effectiveness of different drying methods and to investigate the effects of adding a series of individual protectant such as skim milk, sucrose, maltodextrin, and corn starch for preserving Lactobacillus acidophilus FTDC 3081 cells during spray and freeze-drying and storage at different temperatures. Results showed a remarkable high survival rate of 70–80% immediately after spray- and freeze-drying in which the cell viability retained at the range of 109 to 1010 CFU/mL. After a month of storage, maltodextrin showed higher protective ability on both spray- and freeze-dried cells as compared to other protective agents at 4°C, 25°C, and 40°C. A complete loss in viability of spray-dried L. acidophilus FTDC 3081 was observed after a month at 40°C in the absence of protective agent.
Recent progress of morphable 3D mesostructures in advanced materials
Authors: Fu Haoran , Bai Ke , Huang Yonggang , Zhang Yihui
Published: May 2020

Soft robots complement the existing efforts of miniaturizing conventional, rigid robots, and have the potential to revolutionize areas such as military equipment and biomedical devices. This type of system can accomplish tasks in complex and time-varying environments through geometric reconfiguration induced by diverse external stimuli, such as heat, solvent, light, electric field, magnetic field, and mechanical field. Approaches to achieve reconfigurable mesostructures are essential to the design and fabrication of soft robots. Existing studies mainly focus on four key aspects: reconfiguration mechanisms, fabrication schemes, deformation control principles, and practical applications. This review presents a detailed survey of methodologies for morphable mesostructures triggered by a wide range of stimuli, with a number of impressive examples, demonstrating high degrees of deformation complexities and varied multi-functionalities. The latest progress based on the development of new materials and unique design concepts is highlighted. An outlook on the remaining challenges and open opportunities is provided.
Nanofiber/nanowires-based flexible and stretchable sensors
Authors: Wang Dongyi , Wang Lili , Shen Guozhen
Published: May 2020

Nanofibers/nanowires with one-dimension (1D) nanostructure or well-patterned microstructure have shown distinctly advantages in flexible and stretchable sensor fields, owing to their remarkable tolerance against mechanical bending or stretching, outstanding electronic/optoelectronic properties, good transparency, and excellent geometry. Herein, latest summaries in the unique structure and properties of nanofiber/nanowire function materials and their applications for flexible and stretchable sensor are highlighted. Several types of high-performance nanofiber/nanowire-based flexible pressure and stretchable sensors are also reviewed. Finally, a conclusion and prospect for 1D nanofiber/nanowires-based flexible and stretchable sensors are also intensively discussed. This summary offers new insights for the development of flexible and stretchable sensor based 1D nanostructure in next-generation flexible electronics.
Gastrin Staining in Inflamed Stomach Biopsies Labeled as “Antral” Rarely Detects Atrophic Gastritis
Authors: Dunn Andrew L J , Drage Michael G , Whitney-Miller Christa L , McMahon Loralee A , Gonzalez Raul S
Published: May 2020

Objectives

Autoimmune metaplastic atrophic gastritis (AMAG) is an underrecognized entity, especially in its early stage. This study assessed whether the use of gastrin immunohistochemistry would increase sensitivity for diagnosing early AMAG.

Methods

Three-hundred gastric biopsies were prospectively stained for gastrin by immunohistochemistry. Inclusion criteria included well-oriented gastric mucosa with mucus glands and minimal plasma cell infiltrate not suspected to represent pyloric metaplasia. Patient age, sex, designated location of biopsy, presence or absence of intestinal metaplasia, and clinical information were not criteria. Any case with absence of gastrin-positive endocrine cells reflexed to chromogranin immunohistochemistry. Maloriented biopsies or cases with current Helicobacter infection were excluded.

Results

The 298-patient study cohort comprised 222 females (mean age, 47 years; range, 16-80 years) and 76 males (mean age, 49 years; range, 7-80 years). Biopsies were designated as “antral/antral nodules” (61%), and the rest were labeled “gastric/random stomach” (39%). Nine cases (3%) exhibited absence of gastrin-positive endocrine cells; one of those showed endocrine cell hyperplasia by chromogranin staining.

Conclusions

Pathologists should be aware of the histologic features of early AMAG and meticulously analyze tissue regardless of specimen labeling. Gastrin immunostain is a supplemental diagnostic tool when encountering inflamed antral-appearing specimens.
Evaluating assembly and variant calling software for strain-resolved analysis of large DNA viruses
Authors: Deng Zhi-Luo , Dhingra Akshay , Fritz Adrian , Götting Jasper , Münch Philipp C , Steinbrück Lars , Schulz Thomas F , Ganzenmüller Tina , McHardy Alice C
Published: May 2020

Infection with human cytomegalovirus (HCMV) can cause severe complications in immunocompromised individuals and congenitally infected children. Characterizing heterogeneous viral populations and their evolution by high-throughput sequencing of clinical specimens requires the accurate assembly of individual strains or sequence variants and suitable variant calling methods. However, the performance of most methods has not been assessed for populations composed of low divergent viral strains with large genomes, such as HCMV. In an extensive benchmarking study, we evaluated 15 assemblers and 6 variant callers on 10 lab-generated benchmark data sets created with two different library preparation protocols, to identify best practices and challenges for analyzing such data. Most assemblers, especially metaSPAdes and IVA, performed well across a range of metrics in recovering abundant strains. However, only one, Savage, recovered low abundant strains and in a highly fragmented manner. Two variant callers, LoFreq and VarScan2, excelled across all strain abundances. Both shared a large fraction of false positive variant calls, which were strongly enriched in T to G changes in a ‘G.G’ context. The magnitude of this context-dependent systematic error is linked to the experimental protocol. We provide all benchmarking data, results and the entire benchmarking workflow named QuasiModo, Quasispecies Metric determination on omics, under the GNU General Public License v3.0 (https://github.com/hzi-bifo/Quasimodo), to enable full reproducibility and further benchmarking on these and other data.
Identity as Discourse: ‘The Person in Need of Guardianship’
Authors: Barel Michal , Doron Israel (Issi) , Strier Roni
Published: May 2020

This article presents a critical discussion of the legal institution of adult guardianship in Israel. Based on a critical discourse analysis’ study of official guardianship reports for older adults submitted by social workers to family courts in Israel, findings reveal how guardianship institutional procedures construct the personal life stories of older persons into legalistic guardianship paradigm, and in doing so they create a new identity of ‘a person in need of guardianship’. The article further delineates how ‘a person in need of guardianship’ (as well as a ‘guardian’) is a discursive identity, a subject who never existed prior to the guardianship proceeding, a legal creation which does not reflect the complexities and realities of older persons.
LncRNA BRCAT54 inhibits the tumorigenesis of non-small cell lung cancer by binding to RPS9 to transcriptionally regulate JAK-STAT and calcium pathway genes
Authors: Yang Wenhan , Qian Youhui , Gao Kaiping , Zheng Wenjing , Wu Guodong , He Qihan , Chen Qianqian , Song Yi , Wang Liang , Wang Yejun , Gu Peigui , Chen Bin , Zhai Rihong
Published: May 2020

Objectives

Increasing evidence suggest that long non-coding RNAs (lncRNAs) play critical roles in cancers. However, the expression pattern and underlying mechanisms of lncRNAs in non-small cell lung cancer (NSCLC) remain incompletely understood. This study aimed to elucidate the functions and molecular mechanisms of a certain lncRNA in NSCLC.

Methods

LncRNA microarray was performed to identify differential expressed lncRNAs between pre- and postoperation plasma in NSCLC patients. The expression level of candidate lncRNA in NSCLC tissues, plasma and cells was determined by quantitative real-time PCR (qRT-PCR) and in situ hybridization. The functional roles of lncRNA were assessed in vitro and in vivo. Furthermore, RNA pull-down, RNA immunoprecipitation, microarray, qRT-PCR and rescue assays were conducted to explore the mechanism action of lncRNA in NSCLC cells.

Results

We identified a novel lncRNA (BRCAT54), which was significantly upregulated in preoperative plasma, NSCLC tissues and NSCLC cells, and its higher expression was associated with better prognosis in patients with NSCLC. Overexpression of BRCAT54 inhibited proliferation, migration and activated apoptosis in NSCLC cells. Conversely, knockdown of BRCAT54 reversed the suppressive effects of BRCAT54. Moreover, overexpression of BRCAT54 repressed NSCLC cell growth in vivo. Mechanistically, BRCAT54 directly bound to RPS9. Knockdown of RPS9 substantially reversed the promoting effects of si-BRCAT54 on cell proliferation and enhanced the inhibitive effect of si-BRCAT54 on BRCAT54 expression. In addition, silencing of RPS9 activated JAK-STAT pathway and suppressed calcium signaling pathway gene expressions.

Conclusion

This study identified BRCAT54 as a tumor suppressor in NSCLC. Targeting the BRCAT54 and RPS9 feedback loop might be a novel therapeutic strategy for NSCLC.
Computed tomography coronary angiography for patients with heart failure (CTA-HF): a randomized controlled trial (IMAGE-HF 1C)
Authors: Chow Benjamin J.W. , Coyle Doug , Hossain Alomgir , Laine Mika , Hanninen Helena , Ukkonen Heikki , Rajda Miroslav , Larose Eric , Hartikainen Juha , Mielniczuk Lisa , Kass Malek , Connelly Kim A. , O’Meara Eileen , Garrard Linda , Bishop Helen , Small Gary , Hedman Marja , Coyle Kathryn , Yla-Herttuala Seppo , Knuuti Juhani , Wells George A. , Beanlands Rob S ,   the IMAGE-HF Investigators
Published: May 2020

Aims

This randomized controlled trial sought to determine the financial impact of an initial diagnostic strategy of coronary computed tomography angiography (CCTA) in patients with heart failure (HF) of unknown aetiology. Invasive coronary angiography (ICA) is used to investigate HF patients. CCTA may be a non-invasive cost-effective alternative to ICA. This randomized controlled trial sought to determine the financial impact of an initial diagnostic strategy of coronary computed tomography angiography (CCTA) in patients with heart failure (HF) of unknown aetiology.

Methods and results

This multicentre, international trial enrolled patients with HF of unknown aetiology. The primary outcome was the cost of CCTA vs. ICA strategies at 12 months. Clinical outcomes were also collected. An ‘intention-to-diagnose’ analysis was performed and a secondary ‘as-tested’ analysis was based on the modality received. Two hundred and forty-six patients were randomized (age = 57.8 ± 11.0 years, ejection fraction = 30.1 ± 10.1%). The severity of coronary artery disease was similar in both groups. In the 121 CCTA patients, 93 avoided ICA. Rates of downstream ischaemia and viability testing were similar for both arms. There were no significant differences in the composite clinical outcomes or quality of life measures. The cost of CCTA trended lower than ICA [CDN −$871 (confidence interval, CI −$4116 to $3028)]. Using an ‘as-tested’ analysis, CCTA was associated with a decrease in healthcare costs (CDN −$2932, 95% CI −$6248 to $746).

Conclusion

In patients with HF of unknown aetiology, costs were not statistically different between the CCTA and ICA strategies.

Clinical Trials.gov

NCT01283659
Prognostic importance of mitral e
′ velocity in constrictive pericarditis
Authors: Yang Jeong Hoon , Miranda William R , Nishimura Rick A , Greason Kevin L , Schaff Hartzell V , Oh Jae K
Published: May 2020

Aims 

Increased medial mitral annulus early diastolic velocity (e′) plays an important role in the echocardiographic diagnosis of constrictive pericarditis (CP) and mitral e′ velocity is also a marker of underlying myocardial disease. We assessed the prognostic implication of mitral e′ for long-term mortality after pericardiectomy in patients with CP.

Methods and results 

We studied 104 surgically confirmed CP patients who underwent echocardiography and cardiac catheterization within 7 days between 2005 and 2013. Patients were classified as primary CP (n = 45) or mixed CP (n = 59) based on the clinical history of concomitant myocardial disease. On multivariable analysis, medial e′ velocity and mean pulmonary artery pressure were independently associated with long-term mortality post-pericardiectomy. There were significant differences in survival rates among the groups divided by cut-off values of 9.0 cm/s and 29 mmHg for medial e′ and mean pulmonary artery pressure, respectively (both P < 0.001). Ninety-two patients (88.5%) had elevated pulmonary artery wedge pressure (PAWP) (≥15 mmHg); there was no significant correlation between medial E/e′ and PAWP (r = 0.002, P = 0.998). However, despite the similar PAWP between primary CP and mixed CP groups (21.6 ± 5.4 vs. 21.2 ± 5.8, P = 0.774), all primary CP individuals with elevated PAWP had medial E/e′ <15 as opposed to 34 patients (57.6%) in the mixed CP group (P < 0.001).

Conclusion 

Increased mitral e′ velocity is associated with better outcomes in patients with CP. A paradoxical distribution of the relationship between E/e′ and PAWP is present in these patients but there is no direct inverse correlation between them.
Time trajectory of cardiac function and its relation with survival in patients with light-chain cardiac amyloidosis
Authors: Hwang In-Chang , Koh Youngil , Park Jun-Bean , Yoon Yeonyee E , Kim Hack-Lyoung , Kim Hyung-Kwan , Kim Yong-Jin , Cho Goo-Yeong , Sohn Dae-Won , Lee Seung-Pyo
Published: May 2020

Aims 

We aimed to analyse the time-serial change of cardiac function in light-chain (AL) cardiac amyloidosis patients undergoing active chemotherapy and its relationship with patient outcome.

Methods and results 

Seventy-two patients with AL cardiac amyloidosis undergoing active chemotherapy who had two or more echocardiographic examinations were identified from a prospective observational cohort (n = 34) and a retrospective cohort (n = 38). Echocardiographic parameters were obtained immediately prior to 1–3, 3–6, 6–12, and 12–24 months after the first chemotherapy. Study endpoint was a composite of death or heart transplantation (HT). During a median of 32 months (interquartile range 8–51) follow-up, 33 patients (45.8%) died and 4 patients (5.6%) underwent HT. Echocardiograms immediately prior to the first chemotherapy did not show differences between the patients with adverse events vs. those without. Significant increase in mitral E/e′ ratio and decline in left ventricular global longitudinal strain (LV-GLS) was observed, starting at 3–6 months after the first chemotherapy only in those who experienced adverse events on follow-up, which was also evident in those who responded to chemotherapy. Multivariate analysis demonstrated that B-natriuretic peptide >500 pg/mL and troponin I >0.15 ng/dL at initial diagnosis, hospitalization for heart failure, E/e′ >15, and LV-GLS <10% during follow-up were independent predictors of outcome.

Conclusions 

In AL cardiac amyloidosis patients undergoing active chemotherapy, the deterioration of LV function may occur, starting even at 3–6 months after the first chemotherapy. Serial echocardiography may help identify those who experience a clinical event in the near future despite active chemotherapy.
The Association Between High-Molecular-Weight Adiponectin, Ghrelin and Leptin and Age-Related Cognitive Decline: Results From Longitudinal Aging Study Amsterdam
Authors: van Andel Merel , van Schoor Natasja M , Korten Nicole C , Comijs Hannie C , Heijboer Annemieke C , Drent Madeleine L
Published: May 2020

Background

Age-related cognitive decline has large-scale functional and economic consequences and understanding its’ pathophysiological mechanisms is therefore essential. Previous research has suggested associations between hormones adiponectin, ghrelin and leptin and neurodegenerative disease. However, their association with age-related cognitive decline has not been fully described. We examine the association between serum high-molecular-weight (HMW) adiponectin, ghrelin and leptin and age-related cognitive decline in older adults.

Methods

The associations between HMW adiponectin, ghrelin and leptin and the Mini-Mental-State-Examination, Coding task (Coding), 15 Words Test (15WT) and composite Z-score (general cognitive function) were analyzed by means of a sex-stratified multivariable linear regression analysis in a population-based cohort of 898 older adults at baseline and after 3 years of follow-up.

Results

In women, we found a positive association between HMW adiponectin and general cognitive function at baseline (fully adjusted model composite Z-score standardized regression co-efficient beta [β] = .089, p = .025). After 3 years of follow-up, HMW adiponectin was associated with more decline in general cognitive function and information processing speed (fully adjusted model composite Z-score β = −.123, p = .018; Coding β = −.116, p = .027). Ghrelin and leptin were significantly associated with memory in a baseline subgroup analysis of older women. For men, we found no significant associations at baseline or follow-up.

Conclusion

Our results show variable associations between hormones HMW adiponectin, ghrelin and leptin and age-related cognitive decline in women but not in men. As there was no clear trend, all our results should be interpreted with caution.
Cell-Mediated Immunogenicity of Influenza Vaccination in Patients With Cancer Receiving Immune Checkpoint Inhibitors
Authors: Kang Chang Kyung , Kim Hang-Rae , Song Kyoung-Ho , Keam Bhumsuk , Choi Seong Jin , Choe Pyoeng Gyun , Kim Eu Suk , Kim Nam Joong , Kim Yu Jung , Park Wan Beom , Kim Hong Bin , Oh Myoung-don
Published: May 2020

Background

We assessed cell-mediated immune (CMI) responses of influenza vaccination in patients with cancer receiving immune checkpoint inhibitors (ICIs), which remain elusive.

Methods

Vaccine-elicited CMI responses in patients receiving ICIs or cytotoxic agents were investigated by flow cytometry. Polyfunctional cells were defined as T cells that express 2 or more of interleukin 2 (IL-2), interleukin 4 (IL-4), interferon gamma (IFN-γ), and CD107a. An adequate CMI response was defined as an increase of polyfunctional T cells against both H1N1 and H3N2 strains.

Results

When comparing ICI (n = 11) and cytotoxic chemotherapy (n = 29) groups, H1N1-specific IL-4 or IFN-γ–expressing CD4+ T cells, IL-2, IL-4, IFN-γ, or CD107a-expressing CD8+ T cells, H3N2-specific IFN-γ–expressing CD4+ T cells, and CD107a-expressing CD8+ T cells were more frequent in the ICI group. Fold changes in polyfunctional H3N2-specific CD4+ (median, 156.0 vs 95.7; P = .005) and CD8+ (155.0 vs 103.4; P = .044) T cells were greater in the ICI group. ICI administration was strongly associated with an adequate CMI response for both CD4+ and CD8+ T cells (P = .003).

Conclusions

CMI responses following influenza vaccination were stronger in the ICI group than in the cytotoxic chemotherapy group. Influenza vaccination should be strongly recommended in patients with cancer receiving ICIs.
Burn Wound Microbiology and the Antibiotic Susceptibility Patterns of Bacterial Isolates in Three Burn Units of Abbottabad, Pakistan
Authors: Hubab Muhammad , Maab Hira , Hayat Azam , Ur Rehman Mujaddad
Published: May 2020

Infection is the leading cause of morbidity and mortality among burn patients and is accentuated multifold by the emergence of antimicrobial resistance among the nosocomial isolates. It is vital to know the common organisms involved in infected burn wound etiology and their respective antibiotic susceptibility patterns. These crucial findings can help in formulating a better and more efficient antimicrobial therapy plan for controlling burn wound infections. The current study was conducted to identify the common bacteria involved in causing infections in wounds of burn patients and their respective antibiotic susceptibility patterns in three hospitals of Abbottabad, Pakistan. A total of 100 patients were included from the burn units of three hospitals in Abbottabad. Wound swabs were taken from the deepest portions of infected burns, and the organisms involved were isolated via standard microbiological techniques. The Kirby-Bauer disc diffusion technique was used to monitor antibiotic susceptibility. Gram-positive organisms were found readily in infected burn wounds. Staphylococcus aureus (46%) was the most common isolate followed by Staphylococcus epidermidis (17%), Escherichia coli (16%), Proteus spp. (12%), Klebsiella pneumoniae (10%), and Pseudomonas aeruginosa, which was only 7%. Gram-positive bacteria were sensitive to amikacin, gentamicin, cefotaxime, and norfloxacin. In contrast, the gram-negative isolates were sensitive to amikacin, chloramphenicol, and nalidixic acid. Pseudomonas was resistant to most of the antibiotics tested in the present study.
Biology, Ecology, and Management of Rednecked Peanutworm (Lepidoptera: Gelechiidae)
Authors: Pinto J R L , Boiça A L , Fernandes O A
Published: May 2020

The rednecked peanutworm, Stegasta bosqueella (Chambers), is the main lepidopteran pest in South and Central American peanut fields. The species name was misspelled earlier in the literature and the incorrect name is still more cited. In this review, we revisit the history of S. bosqueella classification. Although the rednecked peanutworm is an important defoliator in some peanut producing countries, there is scarce information on some aspects of the biology as well as plant response to this insect species. Economic threshold levels are not yet well established and, consequently, decision making is compromised. Chemical control is the main tool used to control larval infestations. Moreover, some studies on host plant resistance, biological control, and attract-and-kill strategy were conducted to control the rednecked peanutworm. These tactics were promising and could be useful to enhance a peanut integrated pest management program. We present here a review of S. bosqueella life history, biology, ecology, and management options as well as needs for enhancing the current control strategies.
Human Blood Feeding by Aedes aegypti (Diptera: Culicidae) in the Florida Keys and a Review of the Literature
Authors: Pruszynski Catherine A , Stenn Tanise , Acevedo Carolina , Leal Andrea L , Burkett-Cadena Nathan D
Published: May 2020

Aedes aegypti L. is considered to have a proclivity for feeding on human blood even when other hosts are available. However, few studies have demonstrated host use by this mosquito in the continental United States, where local transmission of dengue, Zika, and chikungunya viruses has been recently documented. This study investigated the bloodmeal sources of female Ae. aegypti in the subtropical city of Key West and the surrounding county in Florida with the goal of identifying preferred hosts. Blood-engorged Ae. aegypti mosquitoes were collected from BG Sentinel traps used as part of a routine surveillance program in the Florida Keys (Monroe County, Florida). Bloodmeal samples were analyzed using PCR assays, sequencing, and comparison with reference sequences in GenBank. Aedes aegypti females from Key West fed predominantly on humans (79.6%) and did not differ statistically from females collected from the rest of the Florida Keys (69.5%). Culex quinquefasciatus Say (Diptera: Culicidae), considered a host generalist, was collected and analyzed from the same sites for comparative purposes. Females of Cx. quinquefasciatus fed predominantly (70.7%) on birds and nonhuman mammals in the Florida Keys, corroborating the validity of molecular assay breadth and demonstrating that given the same group of available hosts Ae. aegypti selects humans. Our results indicated that Ae. aegypti has a high rate of human-biting in a subtropical area within the United States, supporting its role in recent local transmission of dengue and other viruses.
Connexin43 Expression and Associated Chronic Inflammation Presages the Development of Cerebral Radiation Necrosis
Authors: Feldman Lisa A , Haldankar Shewta , O’Carroll Simon J , Liu Karen , Fackelmeier Barbara , Broaddus William C , Anene-Maidoh Tony , Green Colin R , Garbow Joel R , Guan Jian
Published: May 2020

Cerebral radiation necrosis (CRN) is a delayed complication of radiosurgery that can result in severe neurological deficits. The biological changes leading to necrotic damage may identify therapeutic targets for this complication. Connexin43 expression associated with chronic inflammation may presage the development of CRN. A mouse model of delayed CRN was used. The left hemispheres of adult female mice were irradiated with single-fraction, high-dose radiation using a Leksell Gamma Knife. The brains were collected 1 and 4 days, and 1–3 weeks after the radiation. The expression of connexin43, interleukin-1β (IL-1β), GFAP, isolectin B-4, and fibrinogen was evaluated using immunohistochemical staining and image analysis. Compared with the baseline, the area of connexin43 and IL-1β staining was increased in ipsilateral hemispheres 4 days after radiation. Over the following 3 weeks, the density of connexin43 gradually increased in parallel with progressive increases in GFAP, isolectin B-4, and fibrinogen labeling. The overexpression of connexin43 in parallel with IL-1β spread into the affected brain regions first. Further intensified upregulation of connexin43 was associated with escalated astrocytosis, microgliosis, and blood-brain barrier breach. Connexin43-mediated inflammation may underlie radiation necrosis and further investigation of connexin43 hemichannel blockage is merited for the treatment of CRN.
Prognostic Value of Histopathological Features and Loss of H3K27me3 Immunolabeling in Anaplastic Meningioma: A Multicenter Retrospective Study
Authors: Gauchotte Guillaume , Peyre Matthieu , Pouget Celso , Cazals-Hatem Dominique , Polivka Marc , Rech Fabien , Varlet Pascale , Loiseau Hugues , Lacomme Stéphanie , Mokhtari Karima , Kalamarides Michel , Bielle Franck
Published: May 2020

The diagnosis of anaplastic meningioma (AM) (WHO grade III) is based on the presence of a high mitotic index (MI) and/or overt anaplasia. Only few data exist about the reproducibility and prognostic value of overt anaplasia. Additionally, the prognostic value of H3K27me3 loss in AM has not yet been demonstrated. Our objectives were to evaluate the reproducibility and prognostic value of WHO criteria and H3K27me3 loss in a multicenter series of 66 AM. Interobserver reproducibility was good for the determination of WHO grade (Kappa = 0.671) and MI (intraclass correlation coefficient [ICC] = 0.649), and fair for assessment of overt anaplasia (Kappa = 0.366). Patients with meningiomas showing high MI had significantly shorter overall survival (OS) than patients with meningiomas showing overt anaplasia without high MI (p = 0.009). OS was significantly lower in case of overt anaplasia with low MI (<20/1.6 mm2) than in atypical meningiomas (p = 0.008). H3K27me3 loss was present in 10/47 (21%) of AM and independently associated with shorter OS (p = 0.036; Cox multivariate analysis), with a good reproducibility (Kappa = 0.643). In conclusion, the presence of overt anaplasia could give additional prognostic information in tumors lacking high MI. Finally, loss of H3K27me3 is an easy-to-use and reproducible marker of poorer prognosis.
Phase I dose-escalation trial of S-1 combined with carbon-ion radiotherapy for sinonasal squamous cell carcinoma
Authors: Takahashi Daiki , Demizu Yusuke , Park Sung Chul , Matsuo Yoshiro , Sulaiman Nor Shazrina , Terashima Kazuki , Tokumaru Sunao , Akashi Masaya , Okimoto Tomoaki
Published: May 2020

This study aimed to determine the maximum tolerance dose (MTD) and to estimate the recommended dose (RD) of concomitant S-1 with carbon-ion radiotherapy (RT) for sinonasal squamous cell carcinoma (SCC). Nine patients with sinonasal SCC received carbon-ion RT with escalating doses of S-1 according to phase I methods. Doses of 40, 60 and 80 mg/m2/day were administered twice daily in dose levels 1, 2 and 3, respectively, from days 1 to 14 and 22 to 35. Carbon-ion RT was administered at a dose of 70.4 Gy (relative biological effectiveness) in 32 fractions, 5 days a week. Two patients developed grade 3 acute dermatitis. However, none developed dose-limiting toxicities. Therefore, the MTD of S-1 could not be determined; the RD was estimated to be 80 mg/m2/day with concurrent carbon-ion RT. Partial response and stable disease were noted in 5 and 4 patients, respectively. The 2-year overall survival and local control rates were 56 and 74%, respectively. Overall, 2 patients developed ≥grade 3 late toxicities; among them, 1 patient developed grade 3 cataract and the other developed grade 4 cataract, optic nerve disorder and hearing impairment. To the best of our knowledge, this phase I study is the first clinical trial to evaluate concomitant S-1 with carbon-ion RT for sinonasal SCC. The MTD of S-1 could not be determined, and the RD was estimated to be 80 mg/m2/day. This study demonstrated a manageable safety profile for this combination. The observed outcomes may facilitate further evaluation of this novel therapy.
Frontal sinus inverted papilloma managed with Draf III
Authors: Al Mahdi Mohammed Jihad , Asiri Mohammed , Pharaon Majed , Mubark Ali
Published: May 2020

Inverted papilloma (IP) is a rare benign tumor that originates from nasal and paranasal sinus mucosa. Although it is considered a benign lesion, it is locally aggressive and has risk between 7 and 15% for malignant transformation. The management of frontal sinus (FS) IP is still challenging due to its rarity and local invasiveness. We report a rare case of IP originating from FS, and to review the literature to delineate the possible surgical strategy. A 52-year-old male presented with long-standing history of bilateral nasal obstruction and left eye proptosis. Sinus navigation computed tomography showed soft tissue density occupying frontal, ethmoid, right sphenoid sinuses with extension to nasal cavities, postnasal space and left orbital space plus erosive bony changes. Transnasal endoscopic biopsy suggested IP the lesion was completely resected by Draf III procedure. After 1 month of the surgery, the patient is doing well with no complaints.
On the different types of collective attacks in abstract argumentation: equivalence results for SETAFs
Authors: Dvořák Wolfgang , Rapberger Anna , Woltran Stefan
Published: May 2020

Argumentation frameworks with collective attacks are a prominent extension of Dung’s abstract argumentation frameworks, where an attack can be drawn from a set of arguments to another argument. These frameworks are often abbreviated as SETAFs. Although SETAFs have received increasing interest recently, a thorough study on the actual behaviour of collective attacks has not been carried out yet. In particular, the richer attack structure SETAFs provide can lead to different forms of redundant attacks, i.e. attacks that are subsumed by attacks involving less arguments. Also the notion of strong equivalence, which is fundamental in nonmonotonic formalisms to characterize equivalent replacements, has not been investigated for SETAFs so far. In this paper, we first provide a classification of different types of collective attacks and analyse for which semantics they can be proven redundant. We do so for eleven well-established abstract argumentation semantics. We then study how strong equivalence between SETAFs can be decided with respect to the considered semantics and also consider variants of strong equivalence. Our results show that removing redundant attacks in a suitable way provides direct means to characterize strong equivalence by syntactical equivalence of so-called kernels, thus generalizing well-known results on strong equivalence between Dung AFs.
The role of 3′UTR-protein complexes in the regulation of protein multifunctionality and subcellular localization
Authors: Ribeiro Diogo M , Prod’homme Alexis , Teixeira Adrien , Zanzoni Andreas , Brun Christine
Published: May 2020

Multifunctional proteins often perform their different functions when localized in different subcellular compartments. However, the mechanisms leading to their localization are largely unknown. Recently, 3′UTRs were found to regulate the cellular localization of newly synthesized proteins through the formation of 3′UTR-protein complexes. Here, we investigate the formation of 3′UTR-protein complexes involving multifunctional proteins by exploiting large-scale protein-protein and protein-RNA interaction networks. Focusing on 238 human ‘extreme multifunctional’ (EMF) proteins, we predicted 1411 3′UTR-protein complexes involving 54% of those proteins and evaluated their role in regulating protein cellular localization and multifunctionality. We find that EMF proteins lacking localization addressing signals, yet present at both the nucleus and cell surface, often form 3′UTR-protein complexes, and that the formation of these complexes could provide EMF proteins with the diversity of interaction partners necessary to their multifunctionality. Our findings are reinforced by archetypal moonlighting proteins predicted to form 3′UTR-protein complexes. Finally, the formation of 3′UTR-protein complexes that involves up to 17% of the proteins in the human protein-protein interaction network, may be a common and yet underestimated protein trafficking mechanism, particularly suited to regulate the localization of multifunctional proteins.
Residential Stability Versus Residents’ Interactions: The Determinants of Voluntary Participation in Police Block Activity
Authors: Coats Jonathan E
Published: May 2020

This research examines the influence of residential stability and resident’s interactions on participation in police block activity. Community engagement is one approach used by the police to increase the co-production of crime prevention by providing opportunities and engaging the community in group-level informal and voluntary activities. These optional activities are designed to promote a working relationship between the residents and the police. Multilevel ordinal logistic regression was run to estimate participation in police block activity, using the Seattle Neighborhood and Crime Survey data. The results demonstrate two things. First, this study extends the standard variables, used in examining voluntary involvement, to the frequency of participation in police block activity. Secondly, using interaction terms between residential stability and between residents’ interactions, the results suggest that between residents’ interaction may be a better predictor of frequent participation in police block activity than homeownership or length of residency. Finally, policy implications and future research avenues are discussed.
Elevated circulating pro-inflammatory low-density granulocytes in adult-onset Still’s disease
Authors: Liu Yudong , Xia Changsheng , Chen Jiali , Fan Chunhong , He Jing
Published: May 2020

Objectives

Neutrophilia is a hallmark of adult-onset Still’s disease (AOSD). This study aimed to investigate the role of a distinct subset of granulocytes, the low-density granulocytes (LDGs) in the pathogenesis of AOSD.

Methods

A total of 56 patients with AOSD were included in the study. LDGs were quantified by flow cytometry. Correlations between LDGs with disease activity and laboratory parameters were determined by Spearman’s nonparametric test. The cellular sources of the pro-inflammatory cytokines in AOSD were determined by intracellular staining.

Results

Active AOSD patients displayed significantly higher levels of LDGs compared with inactive AOSD patients and healthy controls (HCs) (P<0.001). Circulating LDGs were significantly correlated with CRP, ESR and the modified Pouchot score in patients with AOSD (P<0.01). The levels of LDGs were significantly decreased after the active AOSD patients achieved disease remission (P=0.0391). CD14+ monocytes constituted over 90% IL-1β+ peripheral blood mononuclear cells (PBMCs) and over 80% TNF-α+ PBMCs in both active AOSD patients and HCs, respectively. In active AOSD, CD14+ monocytes accounted for 24.6% to 75.0% of IL-6+ PBMCs, while LDGs comprised 22.8% to 72.2% of IL-6+ PBMCs. In contrast, over 90% IL-6+ PBMCs were CD14+ monocytes in HCs. A significant correlation was identified between the levels of LDGs and serum IL-6 levels in AOSD (P<0.0001).

Conclusion

Active AOSD is associated with elevated levels of a pro-inflammatory subset of neutrophils, the LDGs that produce IL-6. Our data highlight an unappreciated role of LDGs in the aberrant innate immune responses in AOSD.
0152 Insomnia Severity and Daytime Complaints: What is to be Learned When These Domains are Discordant?
Authors: Perlis M L , Boyle J T , Vargas I , Giller J , Seewald M , D’Antonio B , Muench A , Williams N J , Rosenfield B , Klingman K
Published: May 2020

Introduction

If illness severity and daytime dysfunction are construed as categorical entities, it is possible to conceptualize the relationship between these variables in terms of a 2x2 matrix where the resultant cells represent a concordant dimension (quadrants 2 & 4 [high-high and low-low]) and a discordant dimension (quadrants 1 & 3 [high-low and low-high]). The question for the present analysis was, what percentage of subjects populate each quadrant and is it the case that the discordant dimension contains only a small percentage of subjects?

Methods

Illness severity and daytime dysfunction data was collected from individuals with sleep continuity complaints in archival/community-based sample (N = 4680; 60% female; Ages 18–89) (www.sleeplessinphilly.com). Illness severity was operationalized as Total Wake Time (TWT; [SL+WASO+EMA=TWT]) and daytime dysfunction was operationalized as the composite score of six daytime symptoms items. Median splits were calculated for each variable and subjects were typed accordingly (HH, LL, HL, & LH).

Results

Surprisingly, the sample was relatively equally distributed into the two dimensions; 38% and 23% for the concordant dimension and 13% and 26% for discordant dimension.

Conclusion

The 39% of subjects in the discordant groups might be thought of as complaining good sleepers (LH) and noncomplaining poor sleepers (HL). Other investigators have identified the LH subjects as individuals with “insomnia identity”. Alternatively, it is possible to characterize the whole dimension as being related to a mismatch between the individual’s sleep need and sleep ability. Those who need a lot, may suffer a lot, in the face of only a little (LH) whereas those who need a little, may suffer only a little, in the face of a lot (HL).

Support
0174 Examining Circadian Disadvantages in the National Basketball Association’s Playoffs
Authors: Pradhan S , Gregory K , Alton D , Chachad R , Flynn-Evans E E
Published: May 2020

Introduction

Prior research on travel in the National Basketball Association’s (NBA) regular season has shown that teams journeying west relative to their home base face circadian disadvantages for evening games, while those traveling east have advantages. The current study extends previous research by examining these effects within the NBA playoffs. We hypothesized that teams would have a greater circadian advantage during eastward compared to westward travel.

Methods

In 2013, the NBA implemented a 7-game series playoff structure, in which teams play an alternating home/away 2-2-1-1-1 format. Data for all 499 postseason games played during the 2013–14 to 2018–2019 seasons were collected from Basketball-Reference and FiveThirtyEight. We investigated the impact of direction of travel based on home base city (same time zone, westward, eastward) and time zones traveled on game outcomes, Elo rating differences (i.e., a team quality metric based on wins and losses), win probability, and team scoring.

Results

Teams had lower win probabilities following 3-hour westward than same time zone and all eastward travel, while 3-hour eastward travel related to higher probabilities of winning compared to same time and all westward travel (p < .001, d > .95). Teams travelling westward with 2-hour time changes lost significantly more games than those experiencing 1-hour westward (p = .04, OR = 2.45), 1-hour eastward (p = .05, OR = 2.34), and 3-hour eastward changes (p = .02, OR = 4.68). Scoring was significantly higher following eastward travel compared to both westward (p = .001, d = 0.60) and same time zone travel (p = .003, d = 0.44). There were no differences in team quality based on direction of travel or number of time zones traveled, and game outcomes based on overall direction of travel (p > .05).

Conclusion

Direction and magnitude of travel were related to win probability, team scoring, and game outcomes, whereby teams travelling eastward and within the same time zone gained an advantage over those travelling westward. Adjustment to travel and time changes appear to influence in-game performances and outcomes in the NBA playoffs.

Support

None