bluuebunny/crossref_metadata_2025 huggingface dataset:
Transonic flutter characteristics of an airfoil with morphing devices
Authors: He Shun , Guo Shijun , Li Wenhao
Published: August 2020

An investigation into transonic flutter characteristic of an airfoil conceived with the morphing leading and trailing edges has been carried out. Computational fluid dynamics (CFD) is used to calculate the unsteady aerodynamic force in transonic flow. An aerodynamic reduced order model (ROM) based on autoregressive model with exogenous input (ARX) is used in the numerical simulation. The flutter solution is determined by eigenvalue analysis at specific Mach number. The approach is validated by comparing the transonic flutter characteristics of the Isogai wing with relevant literatures before applied to a morphing airfoil. The study reveals that by employing the morphing trailing edge, the shock wave forms and shifts to the trailing edge at a lower Mach number, and aerodynamic force stabilization happens earlier. Meanwhile, the minimum flutter speed increases and transonic dip occurs at a lower Mach number. It is also noted that leading edge morphing has negligible effect on the appearance of the shock wave and transonic flutter. The mechanism of improving the transonic flutter characteristics by morphing technology is discussed by correlating shock wave location on airfoil surface, unsteady aerodynamics with flutter solution.
NeuroRegen Scaffolds Combined with Autologous Bone Marrow Mononuclear Cells for the Repair of Acute Complete Spinal Cord Injury: A 3-Year Clinical Study
Authors: Chen Wugui , Zhang Ying , Yang Sizhen , Sun Jing , Qiu Hao , Hu Xu , Niu Xiaojian , Xiao Zhifeng , Zhao Yannan , Zhou Yue , Dai Jianwu , Chu Tongwei
Published: August 2020

Spinal cord injury (SCI) remains among the most challenging pathologies worldwide and has limited therapeutic possibilities and a very bleak prognosis. Biomaterials and stem cell transplantation are promising treatments for functional recovery in SCI. Seven patients with acute complete SCI diagnosed by a combination of methods were included in the study, and different lengths (2.0–6.0 cm) of necrotic spinal cord tissue were surgically cleaned under intraoperative neurophysiological monitoring. Subsequently, NeuroRegen scaffolds loaded with autologous bone marrow mononuclear cells (BMMCs) were implanted into the cleaned site. All patients participated in 6 months of rehabilitation and at least 3 years of clinical follow-up. No adverse symptoms associated with stem cell or functional scaffold implantation were observed during the 3-year follow-up period. Additionally, partial shallow sensory and autonomic nervous functional improvements were observed in some patients, but no motor function recovery was observed. Magnetic resonance imaging suggested that NeuroRegen scaffold implantation supported injured spinal cord continuity after treatment. These findings indicate that implantation of NeuroRegen scaffolds combined with stem cells may serve as a safe and promising clinical treatment for patients with acute complete SCI. However, determining the therapeutic effects and exact application methods still requires further study.
Molecular screening of XY SRY-negative sex reversal cases in horses revealed anomalies in amelogenin testing
Authors: Martinez Maria M. , Costa Mónica , Ratti Cecilia
Published: August 2020

Male-to-female sex reversal in horses is a developmental disorder in which phenotypic females have a male genetic constitution. Male-to-female sex reversal is the second most common genetic sex abnormality, after X chromosome monosomy. All male-to-female sex reversal cases studied to date have been found to be infertile. Therefore, a screening test is particularly useful in laboratories doing DNA genotyping in horses. Our laboratory has tested > 209,000 horses for parentage using a panel of microsatellite markers and the sex marker gene amelogenin ( AMEL). Suspect XY sex reversal cases are reported females with a male profile by AMEL testing. After routine genotyping, 49 cases were detected and further tested using the sex-determining region Y ( SRY) gene, confirming the XY SRY-negative genotype of suspect sex reversal cases. When some inconsistencies arose in the initial result, a molecular panel of X- and Y-linked markers was analyzed for these samples. Of the 49 cases, 33 were confirmed as XY SRY-negative. The remaining 16 cases were identified as false-positives as a result of anomalies of AMEL testing in horses.
Is It Safe? Community Integration for Individuals With Serious Mental Illnesses
Authors: Pahwa Rohini , Dougherty Ryan J. , Kelly Erin , Davis Lisa , Smith Melissa E. , Brekke John S.
Published: August 2020

Purpose: Community integration is a key component of recovery for individuals with serious mental illnesses (SMIs). The goal of the current study is to explore the nature and impact of safety concerns for experiences of communities for individuals with SMIs. Method: Using constructivist grounded theory, 30 semistructured interviews were analyzed to explore the depth and breadth of individuals’ safety concerns, how individuals manage these concerns, and how these concerns shape their community experiences. Findings: Participants described various unsafe encounters related to neighborhood disorder, homelessness, traumatic experiences, substance use, stigma, and loss of relationships and multiple strategies to manage their safety. Many saw mental health services as a sanctuary from the dangers of their environment, providing support, resources, and coping skills. For many, future communities were envisioned within the context of safety. Conclusions: Service providers should consider safety as a critical issue that impacts how individuals experience their current and future communities.
The Single’s Struggle: Discovering Involuntary Singleness in Indonesia Through Gender and Religious Perspectives
Authors: Himawan Karel Karsten
Published: August 2020

Singleness emerges as a theme in studies on contemporary relationships across societies, including in Indonesia. While in most Western societies, singleness reflects an individual’s personal preference, marriage is viewed as cultural imperative in Indonesia, and being single is often held involuntarily by most never-married adults. This study outlines the reasons of why Indonesian individuals remain involuntarily single. The interviews of 40 never-married adults aged 27–52 years ( M age = 33.14; SD = 4.04) revealed that gender and religious differences regarding marriage expectation are central themes in understanding involuntary singleness. The study particularly revealed four gender-specific reasons for why individuals remained involuntarily single: obtaining a career, having an incompatible marriage expectation, having dependent family members, and having temporal perspectives of singleness. Two themes emerged regarding the religious perspective of singleness: religious interpretations about singleness and religion-related coping ways of being involuntarily single. The themes suggest that marriage is not a mere personal fulfillment as cultural and religious values determine individuals’ marriage feasibility. While offering a new perspective of involuntary singleness from non-Western perspective, the results inform strategies to cope with unwanted singleness, particularly in the marriage- and religion-preoccupied societies.
Update on the Clot Waveform Analysis
Authors: Wada Hideo , Matsumoto Takeshi , Ohishi Kohshi , Shiraki Katsuya , Shimaoka Motomu
Published: August 2020

The activated partial thromboplastin time (APTT)–clot waveform analysis (CWA) was previously reported to be associated with the early detection of disseminated intravascular coagulation and was also reported to be able to measure very low levels of coagulation factor VIII activity. The software program for the analysis for the APTT-CWA allows the associated first and second derivative curves (first and second DCs) to be displayed. The first and second DC reflect the velocity and acceleration, respectively. The height of the first DC reflects the “thrombin burst” and bleeding risk, while that of the second DC is useful for detecting any coagulation factor deficiency and abnormal enhancement of coagulation by phospholipids. Activated partial thromboplastin time-CWA aids in making a differential diagnosis which is difficult to do using only the routine APTT. The CWA is currently used for many applications in the clinical setting, including the monitoring of hemophilia patients and patients receiving anticoagulant therapy and the differential diagnosis of diseases.
The Mediation of Emotions in Sport Events: A Case Study in Badminton
Authors: Cabello-Manrique David , Nuviala Román , Pappous Athanasios (Sakis) , Puga-González Esther , Nuviala Alberto
Published: August 2020

This study examines the relationships between functional quality, outcome quality, satisfaction, and future intentions, influenced by emotions, of spectators who attended the 2018 European Badminton Championships. The population studied was 686 spectators. The mean age was 36.08 ± 14.15 years, 39.1% were females, and 66.1% were local resident spectators. The results allow to affirm that functional quality and outcome quality have an impact on satisfaction, either directly or indirectly through emotions. In the same way, it has been possible to observe the relationship between satisfaction and future intentions of the spectators. These results help understand the factors that predict the loyalty of spectators of sports events of badminton.
Low pelvic incidence is a risk factor for intraoperative complications in minimally invasive anterolateral approach for total hip arthroplasty
Authors: Kim Youngwoo , Pour Aidin Eslam , Lazennec Jean Yves
Published: August 2020

Background:

Minimally invasive anterolateral approach (ALA) has gained popularity in recent years as better postoperative functional recovery and lower risk of postoperative dislocation are claimed. However, difficulties for femur exposure and intraoperative complications during femoral canal preparation and component placement have been reported. This study analyses the effect of anatomical factors on difficulties for femoral access and intraoperative complications with a modified minimally invasive ALA.

Material and methods:

310 consecutive patients who had primary unilateral total hip arthroplasty (THA) by single surgeon using same approach and technique, and being suitable for EOS were included. All patients underwent pre- and postoperative standing and sitting full-body EOS acquisitions and pelvic and femoral parameters were measured. All intra- and postoperative complications for femoral preparation and implantation were assessed. Intraoperative complications included femoral fractures and difficulties for femoral exposure.

Results:

10 patients (3.2%) had intraoperative femoral fractures (2 greater trochanter, 8 calcar). Difficult access to the proximal femur was reported for 10 other patients (3.2%). Patients with intraoperative complications presented a significantly lower pelvic incidence (PI) than patients without intraoperative complications (mean PI: 39.4° vs. 56.9°, p < 0.001).

Conclusions:

The pelvic incidence can be useful to detect anatomically less favourable patients for THA implantation using ALA.
Substance Use Rates of Veterans with Depression Leaving Incarceration: A Matched Sample Comparison with General Veterans
Authors: Spence Nicholas R , Crawford April , LePage James P
Published: August 2020

Formerly incarcerated military veterans—comprising approximately 8% of the prison population—experience significant barriers to reentry including homelessness, mental illness, and mortality. One of the most consequential barriers is relapsing into substance use. Most justice-involved veterans (JIV) possess histories of substance abuse before imprisonment, and this rate continues upon release. Mental illness—depression in particular—is often comorbid with substance abuse. With high rates of depression and substance use in a JIV population, it is important to determine to what extent release from prison contributes to predicting substance use and negative clinical outcomes. This study compares rates of substance use and negative outcomes between two matched samples of JIV and general veterans, both with depression and a substance use disorder. It is hypothesized that JIV will have a higher rate of substance use and that use will be associated with higher negative outcomes in the JIV condition. Rates of use of veterans released from prison and general veterans were collected during six-year follow-up periods. Use was defined as the use of an illicit substance or alcohol, if an alcohol use disorder was present, and determined by urine drug tests or self-report recorded in medical notes. Results indicate that JIV used substances at a higher rate compared to those not recently incarcerated, and that such use is predictive of several other negative outcomes including homelessness, use of inpatient substance treatment, and reincarceration. Structured drug treatment programs and other interventions should cater to veterans recently released from prison to offer help in these areas.
Exploring peer feedback processes and peer feedback meta-dialogues with learners of academic and business English
Authors: Banister Chris
Published: August 2020

Peer feedback has been proposed as a way to boost student feedback literacy and learners’ evaluative judgment. However, the notion of peer feedback and its related processes present numerous challenges for teachers and learners. By adopting the principles of Exploratory Practice with my undergraduates studying academic and business English, I explored why my learners did not seem to view peer feedback as worthwhile, unpacking their attitudes and beliefs in this area of practice using both traditional surveys and the idea of classroom meta-dialogues. I attempted to develop evaluative judgment skills and proactive recipience by tailoring peer feedback processes around oral presentations, a central course component and assessment task for both groups of learners. Survey responses, classroom discussion (the meta-dialogues), observation, and reflection revealed that, overall, my learners were positively predisposed to peer feedback whilst, at the same time, only moderately enthusiastic about the usefulness of the actual peer feedback they received. In a minority of cases, learners resisted peer feedback processes strongly, reflecting the complex interplay of linguistic, cultural and affective factors that represent peer feedback in practice. My learners, at first, struggled to be proactively recipient but helped to identify tensions around tailoring peer feedback. I reflect on the learner resistance encountered and the personal implications for my own teaching. I also suggest ways that language teachers can scaffold proactive recipience and enact tailored peer feedback in a way that is inclusive of both composers’ and recipients’ needs. Finally, I provide a practitioner vantage point on peer feedback, contributing to a diversity of research perspectives in this area.
Health-Related Quality of Life after Hip and Knee Arthroplasty Operations
Authors: Miettinen Hannu J. A. , Mäkirinne-Kallio Ninna , Kröger Heikki , Miettinen Simo S. A.
Published: August 2020

Background and objective:

The aim of this study was to investigate the health-related quality of life before and after a hip and a knee arthroplasty operation using a 15D instrument and to compare these scores to the Finnish control population 15D scores.

Methods:

The pre- and post-operative data of 15D were prospective collected from the patients undergoing total hip arthroplasty or total knee arthroplasty at the Kuopio University Hospital. Post-operative data were collected at 6 and 12 months after the operation.

Results:

The mean change of the 15D score after hip arthroplasty was +0.062 and after knee arthroplasty, it was +0.033 at the 12-month follow-up ( p < 0.001). Total hip arthroplasty patients of all ages reached the control population 15D scores at the 12-month follow-up. Of the total knee arthroplasty patients, only patients aged >75 years and males aged 55–64 years did reach control population 15D scores at the 12-month follow-up. Patients experienced a statistically significant improvement in mobility, vision, sleeping, usual activities, discomfort and symptoms, distress, and vitality ( p < 0.05).

Conclusions:

Successful hip and knee arthroplasty operations improve patients’ health-related quality of life. According to this study, hip arthroplasty improves the health-related quality of life more than knee arthroplasty.
Safety and security concerns at the beach: Views of migrant visitors in Ghana
Authors: Preko Alexander
Published: August 2020

Tourism-migration, safety and security experience are constructs that have drawn the attention of researchers. The literature has acknowledged the importance of safety and security as the foundations for building wealthy tourism. Utilizing the theory of optimism-pessimism, this research sampled 347 migrant visitors of three selected beaches in Ghana’s capital, Accra. The analysis of the chi square test of independence shows a significant association among socio-demographic characteristics (marital status, age categories, education and nationality) and safety and security concerns. However, there is no significant association between gender and safety and security concerns. In all, the findings showed that migrant visitors were optimistic about Ghana’s beach destinations. The results of the study validate the contribution of migrant visitors’ viewpoint on safety and security which is relevant to Ghana’s tourism development. Theoretical and practical implications of the findings are discussed.
Bridging Stent Placement in Vena Cava Syndrome With Tumor Thrombotic Extension in the Right Atrium
Authors: Zander Tobias , Caro Vanesa Di , Maynar Manuel , Rabellino Martin
Published: August 2020

Venous stent placement of symptomatic occlusion of the superior and inferior vena cava is considered the treatment of choice in malignant disease because this technique can restore patency and achieve a relief of symptoms. However, tumor thrombus extension into the right atrium harbors the potential risk of stent migration and perforation. One strategy to avoid this potential life-threatening complication could be the placement of a bridging stent from the superior vena cava-to-inferior vena cava. This case reports describes the superior vena cava-to-inferior vena cava bridging stent technique in 2 patients with malignant occlusion of the superior and inferior vena cava. Special considerations such as technical details of the devices and potential complications are discussed.
Accurate moving object segmentation in unconstraint videos based on robust seed pixels selection
Authors: Zhang Wenlong , Sun Xiaoliang , Yu Qifeng
Published: August 2020

Due to the clutter background motion, accurate moving object segmentation in unconstrained videos remains a significant open problem, especially for the slow-moving object. This article proposes an accurate moving object segmentation method based on robust seed selection. The seed pixels of the object and background are selected robustly by using the optical flow cues. Firstly, this article detects the moving object’s rough contour according to the local difference in the weighted orientation cues of the optical flow. Then, the detected rough contour is used to guide the object and the background seed pixel selection. The object seed pixels in the previous frame are propagated to the current frame according to the optical flow to improve the robustness of the seed selection. Finally, we adopt the random walker algorithm to segment the moving object accurately according to the selected seed pixels. Experiments on publicly available data sets indicate that the proposed method shows excellent performance in segmenting moving objects accurately in unconstraint videos.
Aerodynamic analysis and control for a novel coaxial ducted fan aerial robot in ground effect
Authors: Ai Tianfu , Xu Bin , Xiang Changle , Fan Wei , Zhang Yibo
Published: August 2020

Modeling and control for a novel coaxial ducted fan aerial robot in-ground-effect is presented in this article. Based on experiments using the ducted fan bench test, the fitting curve of the ground effect thrust of the ducted fan aerial robot at different heights is obtained. In addition, the flow field simulation results of the prototype with ground effect at different heights can be obtained using computational fluid dynamics software. A simplified model of the prototype for control can be designed based on several reasonable hypotheses that are established using blade element and momentum theory. To compensate for the disturbance associated with ground effect, a nonlinear disturbance observer is designed to estimate the disturbance, and control structure of the closed-loop system is composed of a nonlinear disturbance observer combined with a double-loop proportion–integration–differentiation controller. The results of several numerical simulations and experiments demonstrate the effectiveness of this controller structure. The performances of tracking trajectory and system stability are improved significantly, compared to the situation that the ground effect is not compensated for.
Financializing the soul: Christian microfinance and economic missionization in Colombia
Authors: Bartel Rebecca C
Published: August 2020

Microfinance is the vanguard of financialization today. This is especially true in Colombia, where microfinance rivals any other type of formal credit. Entangled with Colombia’s micro-financialization is the phenomenon of microfinance corporations in joint ventures with Christian organizations that broker their microfinance programs. These faith-based corporations temper the surge in microfinance with ascetic discipline and the infusion of an entrepreneurial spirit. Economic discipline, say the microfinanciers, is required for what is referred to as ‘financial literacy’ and ‘financial inclusion’ programs that instil a distinctly Christian corporate order. This article, based on 2 years of sustained fieldwork in Colombia, focuses on one such microfinance program run by a transnational Christian credit organization. With microfinance, souls are disciplined through debts and ideals of an ascetic prosperity. In the end, the article concludes that there is a Christian morality to financialized capitalism that is exercised at the level of the interior soul.
Young adolescents’ interest in a mental health casual video game
Authors: Pine Russell , Sutcliffe Kylie , McCallum Simon , Fleming Theresa
Published: August 2020

Background

Mental distress and disorders among adolescents are well documented. Despite the array of treatments available, many mental health issues remain untreated and often undiagnosed. In an attempt to narrow the treatment gap, researchers have adapted existing mental health interventions into digital formats. Despite their efficacy in trial settings, however, real-world uptake of digital mental health interventions is typically low. Casual video games (CVGs) are popular among adolescents and may be a promising tool to reduce stress, anxiety and depression.

Aim

We set out to explore young adolescents’ views of CVGs and their opinions of mental health CVG prototypes, to help determine whether this idea warrants further investigation.

Methods

Pen and paper feedback forms following a brief presentation to 13–15-year-old adolescents in seven high schools (n = 207) followed by more detailed focus groups (n = 42) and workshops (n = 21) with interested students.

Findings

Across all three methods, participants reported playing CVGs several times a week or day to help relieve stress, feel more relaxed and relieve boredom. Most were also interested in the idea of a mental health CVG. Participants in focus groups and workshops confirmed that playing CVGs was common among themselves and their peers, and that the idea of a CVG with subtle and brief mental health content such as game-linked ‘micro messages’ was appealing. Participants recommended that the game should have an engaging interface and subtle mental health skills and information.

Conclusions

Findings from this exploratory study suggest that the concept of a mental health CVG appears to be appealing to adolescents. This novel approach should be tested.
Competency requirements for the assessment of patients with mental illness in somatic emergency care: A modified Delphi study from the nurses’ perspective
Authors: Andersson Henrik , Carlsson Jonas , Karlsson Lene , Holmberg Mats
Published: August 2020

Patients suffering from mental illness are vulnerable, and they do not always have access to proper emergency care. The aim of this study was to identify competency requirements for the assessment of patients with mental illness by soliciting the views of emergency care nurses. A modified Delphi method comprising four rounds was used. Data were collected in Sweden between October 2018 and March 2019. The data were analyzed using content analysis and descriptive statistics. The panel of experts reached the highest level of consensus regarding basic medical knowledge: the capability to listen and show respect to the patient are essential competency requirements when assessing patients with mental illness in emergency care. Awareness of these competency requirements will enhance teaching and training of emergency care nurses.
A PML/RARα direct target atlas redefines transcriptional deregulation in acute promyelocytic leukemia
Authors: Tan Yun , Wang Xiaoling , Song Huan , Zhang Yi , Zhang Rongsheng , Li Shufen , Jin Wen , Chen Saijuan , Fang Hai , Chen Zhu , Wang Kankan
Published: August 2020

Transcriptional deregulation initiated by oncogenic fusion proteins plays a vital role in leukemia. The prevailing view is that the oncogenic fusion protein promyelocytic leukemia/retinoic acid receptor-α (PML/RARα), generated by the chromosome translocation t(15;17), functions as a transcriptional repressor in acute promyelocytic leukemia (APL). Here, we provide rich evidence of how PML/RARα drives oncogenesis through both repressive and activating functions, particularly the importance of the newly identified activation role for the leukemogenesis of APL. The activating function of PML/RARα is achieved by recruiting both abundant P300 and HDAC1 and by the formation of super-enhancers. All-trans retinoic acid and arsenic trioxide, 2 widely used drugs in APL therapy, exert synergistic effects on controlling super-enhancer-associated PML/RARα-regulated targets in APL cells. We use a series of in vitro and in vivo experiments to demonstrate that PML/RARα-activated target gene GFI1 is necessary for the maintenance of APL cells and that PML/RARα, likely oligomerized, transactivates GFI1 through chromatin conformation at the super-enhancer region. Finally, we profile GFI1 targets and reveal the interplay between GFI1 and PML/RARα on chromatin in coregulating target genes. Our study provides genomic insight into the dual role of fusion transcription factors in transcriptional deregulation to drive leukemia development, highlighting the importance of globally dissecting regulatory circuits.
Comparative transcriptome combined with metabolome analyses revealed key factors involved in nitric oxide (NO)-regulated cadmium stress adaptation in tall fescue
Authors: Zhu Huihui , Ai Honglian , Hu Zhengrong , Du Dongyun , Sun Jie , Chen Ke , Chen Liang
Published: August 2020

Background

It has been reported that nitric oxide (NO) could ameliorate cadmium (Cd) toxicity in tall fescue; however, the underlying mechanisms of NO mediated Cd detoxification are largely unknown. In this study, we investigated the possible molecular mechanisms of Cd detoxification process by comparative transcriptomic and metabolomic approaches.

Results

The application of Sodium nitroprusside (SNP) as NO donor decreased the Cd content of tall fescue by 11% under Cd stress (T1 treatment), but the Cd content was increased by 24% when treated with Carboxy-PTIO (c-PTIO) together with Nitro-L-arginine methyl ester (L-NAME) (T2 treatment). RNA-seq analysis revealed that 904 (414 up- and 490 down-regulated) and 118 (74 up- and 44 down-regulated) DEGs were identified in the T1 vs Cd (only Cd treatment) and T2 vs Cd comparisons, respectively. Moreover, metabolite profile analysis showed that 99 (65 up- and 34-down- regulated) and 131 (45 up- and 86 down-regulated) metabolites were altered in the T1 vs Cd and T2 vs Cd comparisons, respectively. The integrated analyses of transcriptomic and metabolic data showed that 81 DEGs and 15 differentially expressed metabolites were involved in 20 NO-induced pathways. The dominant pathways were antioxidant activities such as glutathione metabolism, arginine and proline metabolism, secondary metabolites such as flavone and flavonol biosynthesis and phenylpropanoid biosynthesis, ABC transporters, and nitrogen metabolism.

Conclusions

In general, the results revealed that there are three major mechanisms involved in NO-mediated Cd detoxification in tall fescue, including (a) antioxidant capacity enhancement; (b) accumulation of secondary metabolites related to cadmium chelation and sequestration; and (c) regulation of cadmium ion transportation, such as ABC transporter activation. In conclusion, this study provides new insights into the NO-mediated cadmium stress response.
Overexpression of MaTPD1A impairs fruit and pollen development by modulating some regulators in Musa itinerans
Authors: Hu Chunhua , Sheng Ou , Dong Tao , Yang Qiaosong , Dou Tongxin , Li Chunyu , He Weidi , Gao Huijun , Yi Ganjun , Deng Guiming , Bi Fangcheng
Published: August 2020

Background

Pollen formation and development is important for crop fertility and is a key factor for hybrid development. Previous reports have indicated that Arabidopsis thaliana TAPETUM DETERMINANT1 (AtTPD1) and its rice (Oryza sativa) homolog, OsTPD1-like (OsTDL1A), are required for cell specialization and greatly affect pollen formation and development. Little is known about the role of the TPD1 homolog in banana pollen development.

Results

Here, we report the identification and characterization of TPD1 homologs in diploid banana (Musa itinerans) and examine their role in pollen development by overexpressing the closest homolog, MaTPD1A. MaTPD1A exhibits high expression in stamen and localizes in the plasma membrane. MaTPD1A-overexpressing plants produce no pollen grains and smaller and seedless fruit compared to wild-type plants. Transcriptome analysis showed that in plant hormone, starch and sucrose metabolism, and linolenic acid metabolism-related pathways were affected by overexpression of MaTPD1A, and the expression of several key regulators, such as PTC1 and MYB80, which are known to affect anther development, is affected in MaTPD1A-overexpressing lines.

Conclusions

Our results indicate that MaTPD1A plays an important role in pollen formation and fruit development in diploid banana, possibly by affecting the expression of some key regulators of pollen development.
Intraventricular muscle bundle as a novel cause of left ventricular aneurysm
Authors: Pei Junyu , Zheng Xiaoyan , Liu Yuting , Peng Daoquan , Yu Bilian
Published: August 2020

Background

There are a variety of causes of left ventricular aneurysm, but it is rarely due to a disturbance in intraventricular hemodynamics. To the best of our knowledge, there have been no reports of ventricular aneurysm at the left ventricular apex caused by an abnormal left ventricular muscle bundle.

Case presentation

We report two cases of patients with congenital abnormal left ventricular muscle bundles which caused disturbances in intraventricular hemodynamics. This process eventually led to a left ventricular aneurysm at the apex of the heart. In both cases, transthoracic echocardiography (TTE) and cardiac magnetic resonance imaging (CMR) indicated ventricular aneurysm formation at the apex of the left ventricle. There were also abnormal muscular bundles connecting the ventricular septum and the posterior wall of the left ventricle. The only differences between these two cases were the comorbidities and severity of symptoms.

Conclusion

Ventricular aneurysm at the apex of the left ventricle is common. However, it is rare for a ventricular aneurysm to form due to intraventricular hemodynamic disturbances caused by an abnormal muscle bundle as opposed to that due to original ventricular wall damage, which is more common. There is currently a lack of relevant studies on the treatment and prognosis of such patients. Whether surgical resection of a ventricular aneurysm leads to a better prognosis remains uncertain.
Statistical design of Phase II/III clinical trials for testing therapeutic interventions in COVID-19 patients
Authors: Rai Shesh N. , Qian Chen , Pan Jianmin , Seth Anand , Srivastava Deo Kumar , Bhatnagar Aruni
Published: August 2020

Background

Because of unknown features of the COVID-19 and the complexity of the population affected, standard clinical trial designs on treatments may not be optimal in such patients. We propose two independent clinical trials designs based on careful grouping of patient and outcome measures.

Methods

Using the World Health Organization ordinal scale on patient status, we classify treatable patients (Stages 3–7) into two risk groups. Patients in Stages 3, 4 and 5 are categorized as the intermediate-risk group, while patients in Stages 6 and 7 are categorized as the high-risk group. To ensure that an intervention, if deemed efficacious, is promptly made available to vulnerable patients, we propose a group sequential design incorporating four factors stratification, two interim analyses, and a toxicity monitoring rule for the intermediate-risk group. The primary response variable (binary variable) is based on the proportion of patients discharged from hospital by the 15th day. The goal is to detect a significant improvement in this response rate. For the high-risk group, we propose a group sequential design incorporating three factors stratification, and two interim analyses, with no toxicity monitoring. The primary response variable for this design is 30 day mortality, with the goal of detecting a meaningful reduction in mortality rate.

Results

Required sample size and toxicity boundaries are calculated for each scenario. Sample size requirements for designs with interim analyses are marginally greater than ones without. In addition, for both the intermediate-risk group and the high-risk group, the required sample size with two interim analyses is almost identical to analyses with just one interim analysis.

Conclusions

We recommend using a binary outcome with composite endpoints for patients in Stage 3, 4 or 5 with a power of 90% to detect an improvement of 20% in the response rate, and a 30 day mortality rate outcome for those in Stage 6 or 7 with a power of 90% to detect 15% (effect size) reduction in mortality rate. For the intermediate-risk group, two interim analyses for efficacy evaluation along with toxicity monitoring are encouraged. For the high-risk group, two interim analyses without toxicity monitoring is advised.
Clinical features and outcomes of pregnant women with COVID-19: a systematic review and meta-analysis
Authors: Gao Yi-jie , Ye Lei , Zhang Jia-shuo , Yin Yang-xue , Liu Min , Yu Hong-biao , Zhou Rong
Published: August 2020

Background

The recent COVID-19 outbreak in Wuhan, China, has quickly spread throughout the world. In this study, we systematically reviewed the clinical features and outcomes of pregnant women with COVID-19.

Methods

PubMed, Web of Science, EMBASE and MEDLINE were searched from January 1, 2020, to April 16, 2020. Case reports and case series of pregnant women infected with SARS-CoV-2 were included. Two reviewers screened 366 studies and 14 studies were included. Four reviewers independently extracted the features from the studies. We used a random-effects model to analyse the incidence (P) and 95% confidence interval (95% CI). Heterogeneity was assessed using the I2 statistic.

Results

The meta-analysis included 236 pregnant women with COVID-19. The results were as follows: positive CT findings (71%; 95% CI, 0.49–0.93), caesarean section (65%; 95% CI, 0.42–0.87), fever (51%; 95% CI, 0.35–0.67), lymphopenia (49%; 95% CI, 0.29–0.70), coexisting disorders (33%; 95% CI, 0.21–0.44), cough (31%; 95% CI, 0.23–0.39), fetal distress (29%; 95% CI, 0.08–0.49), preterm labor (23%; 95% CI, 0.14–0.32), and severe case or death (12%; 95% CI, 0.03–0.20). The subgroup analysis showed that compared with non-pregnant patients, pregnant women with COVID-19 had significantly lower incidences of fever (pregnant women, 51%; non-pregnant patients, 91%; P < 0.00001) and cough (pregnant women, 31%; non-pregnant patients, 67%; P < 0.0001).

Conclusions

The incidences of fever, cough and positive CT findings in pregnant women with COVID-19 are less than those in the normal population with COVID-19, but the rate of preterm labor is higher among pregnant with COVID-19 than among normal pregnant women. There is currently no evidence that COVID-19 can spread through vertical transmission.
Molecular epidemiology and clinical characteristics of hepatitis delta virus (HDV) infected patients with elevated transaminases in Shanghai, China
Authors: Wu Shanshan , Zhang Yi , Tang Yuyan , Yao Ting , Lv Mengjiao , Tang Zhenghao , Zang Guoqing , Yu Yongsheng , Chen Xiaohua
Published: August 2020

Background

Patients coinfected with HBV and hepatitis D virus (HDV) have a greater risk of HCC and cirrhosis. The current study was undertaken to assess HDV genotype distribution and determine clinical characteristics of hepatitis delta virus (HDV) among HBsAg positive individuals in Shanghai.

Method

This retrospective study involved 225 serum samples from HBsAg positive hospitalized patients from October 2010 to April 2013. HDV-specific RT-nested PCR was used to amplify HDV RNA. HDV genotypes were characterized by Next-generation sequencing (NGS), followed by phylogenetic analyses. HDV/HBV co-infected patients and HBV mono-infected patients were compared clinically and virologically.

Results

Out of the 225 HBsAg-positive serum samples with elevated transaminases, HDV-RNA was identified in 11 (4.9%) patients. The HBV loads in the HDV positive group were significantly lower than the HDV negative HBV-infected patients. The aminotransferase enzymes were significantly higher in HDV/HBV co-infected compared to HDV negative patients (P < 0.05). Phylogenetic analyses indicated that HDV-2 genotype being the predominant genotype, other HDV genotypes were not observed. HDV/HBV patients were significantly associated with a rather unfavourable clinical outcome.

Conclusion

In summary, the prevalence of HDV infection in patients with elevated transaminases is not low and the predominance of HDV genotype 2 infection in Shanghai. This finding helps us to better understand the correlation of HDV/HBV co-infection. Moreover, Next-generation sequencing (NGS) technologies provide a rapid, precise method for generating HDV genomes to define infecting genotypes.
Herpetic esophagitis in immunocompentent host: cases report
Authors: Diezma-Martín Alba M. , Gigante-Miravalles Esther , Castro Limo Juan Diego , Quimbayo Arcila Carlos Andrés , Puche Paniagua Juan José
Published: August 2020

Background

Herpetic esophagitis (EH) usually affects those who are immunocompromised and is uncommon in immunocompetent patients. In these cases, EH may occasionally present as an acute and self-limited illness. Such cases are rare and only a few have beenreported and limited published reviews exist making the benefits of antiviral therapy in immunocompetent patients unknown.

Case presentation

We report four cases of young patients who presented dysphagia, odynophagia and epigastric pain. Endoscopic findings revealed lesions in the distal esophagus and histopathological changes compatible with herpes virus infection confirmed by viral DNA in every case. After treatment, every patient showed significant improvement and tolerated oral intake after discharge.

Conclusions

In this publication, we present four immunocompetent patients with EH, without relevant alterations in laboratory workup and with negative HIV status. This disease is infrequent in patients with such characteristics and there are few cases published. In order to better understand this pathology, we present the symptoms, the endoscopic alterations and the clinical evolution with treatment. In our series, 50% of patients had serology compatible with acute HVS type 1 infection, 25% had a subacute infection pattern (IgM and IgG positive antibodies) and in another 25% of patients, serology was not done. No patient presented leukocyte alterations, while all patients presented with anatomopathological findings compatible with acute herpetic esophagitis and responded to acyclovir therapy.
Long-term course of anterior spinal cord herniation presenting with an upper motor neuron syndrome: case report illustrating diagnostic and therapeutic implications
Authors: Regensburger Martin , Schlachetzki Johannes C. M. , Klekamp Jörg , Doerfler Arnd , Winkler Jürgen
Published: August 2020

Background

Anterior spinal cord herniation (aSCH) is a rare cause of myelopathy which may present as pure motor syndrome and mimic other degenerative diseases of the spinal cord. In slowly progressive cases, diagnosis may be impeded by equivocal imaging results and mistaken for evolving upper motor neuron disease. As early imaging studies are lacking, we aimed to provide a detailed description of imaging and neurophysiology findings in a patient with aSCH, focusing on the early symptomatic stages.

Case presentation

We here present the case of a 51-year old male patient with an episode of pain in the right trunk and a normal spinal MRI. After a symptom-free interval of 8 years, spasticity and paresis evolved in the right leg. There was subtle ventral displacement and posterior indentation of the thoracic spinal cord on MRI which, in retrospect, was missed as an early sign of aSCH. After another 3 years, symptoms spread to the left leg and a sensory deficit of the trunk became evident. Follow-up MRI now clearly showed an aSCH. Neurosurgical intervention consisted of remobilization of the herniated spinal cord and patch closure of the dura defect. Over the following years, motor and sensory symptoms partially improved.

Conclusions

The history of this patient with aSCH illustrates the importance of careful longitudinal clinical follow-up with repeated imaging studies in progressive upper motor neuron syndromes. Specific attention should be paid to a history of truncal pain and to MRI findings of a ventrally displaced spinal cord. Neurosurgical intervention may halt the progression of herniation.
Multi-service prevention programs for pregnant and parenting women with substance use and multiple vulnerabilities: Program structure and clients’ perspectives on wraparound programming
Authors: Rutman Deborah , Hubberstey Carol , Poole Nancy , Schmidt Rose A. , Van Bibber Marilyn
Published: August 2020

Background

In Canada, several community-based, multi-service programs aimed at reaching vulnerable pregnant or parenting women with substance use and complex issues have emerged. These programs offer basic needs and social supports along with perinatal, primary, and mental health care, as well as substance use services. Evaluations of these ‘one-stop’ programs have demonstrated positive outcomes; nevertheless, few published studies have focused on how these programs are structured, on their cross-sectoral partnerships, and on clients’ perceptions of their services.

Methods

TheCo-Creating Evidence(CCE) project was a three-year evaluation of eight multi-service programs located in six Canadian jurisdictions. The study used a mixed-methods design involving semi-structured interviews, questionnaires, output data, and de-identified client data. This article focuses on qualitative interviews undertaken with 125 clients during the first round of site visits, supplemented by interview data with program staff and service partners.

Results

Each of the programs in the CCE study employs a multi-service model that both reflects a wrap-around approach to care and is intentionally geared to removing barriers to accessing services. The programs are either operated by a health authority (n = 4) or by a community-based agency (n = 4). The programs’ focus on the social determinants of health, and their provision of primary, prenatal, perinatal and mental health care services is essential; similarly, on-site substance use and trauma/violence related services is pivotal. Further, programs’ support in relation to women’s child welfare issues promotes collaboration, common understanding of expectations, and helps to prevent child/infant removals.

Conclusions

The programs involved in the Co-Creating Evidence study have impressively blended social and primary care and prenatal care. Their success in respectfully and flexibly responding to women’s diverse needs, interests and readiness, within a community-based, wraparound service delivery model paves the way for others offering pre- and postnatal programming.
Healthy eating and physical activity among breastfeeding women: the role of misinformation
Authors: Snyder Kailey , Pelster Aja Kneip , Dinkel Danae
Published: August 2020

Background

Eating healthy, being physically active and breastfeeding can greatly support a new mother’s physiological and psychological health. However, within the United States, only 8.5% of women are meeting fruit and vegetable recommendations and only 35% of women are maintaining their pre-pregnancy physical activity after childbirth. Preliminary investigations suggest barriers such as lack of time and knowledge hinder a breastfeeding woman’s ability to eat well and be physically active. The purpose of this study was to explore women’s perceptions of healthy eating and physical activity while breastfeeding.

Methods

This qualitative study utilized a 33-question semi-structured interview guide developed using the constructs of Self-Determination Theory. Data were analyzed via the process of immersion/crystallization.

Results

A total of 24 breastfeeding women completed a roughly 40-min telephone interview. The mean age of the mother was 32 ± .88 and the mean age of the child being breastfed was 8.5 ± 1.4 months old. Findings demonstrate mothers see value in engaging in physical activity primarily for reasons related to self-care. In addition, breastfeeding women have a lack of information and support regarding healthy eating and physical activity while breastfeeding and many are receiving misinformation through resources such as Facebook support groups. Finally, mothers want more resources available that focus specifically on healthy behaviors while breastfeeding.

Conclusion

Women need greater access to education and resources regarding healthy eating and physical activity while breastfeeding. Ideally, information and resources would come from an educated health professional such as their healthcare provider rather than an internet source.