bluuebunny/crossref_metadata_2025 huggingface dataset:
Safety and Tolerability of tDCS across Different Ages, Sexes, Diagnoses, and Amperages: A Randomized Double-Blind Controlled Study
Authors: Buchanan Derrick M. , Amare Sarah , Gaumond Genevieve , D’Angiulli Amedeo , Robaey Philippe
Published: June 2023

Transcranial direct current stimulation (tDCS) is a non-invasive brain stimulation technique with substantial evidence for its safety and tolerability in adults. However, less than 5% of published tDCS research is in pediatrics. Our primary objective was to investigate tDCS safety, tolerability, and acceptability in a sample of children and adults. We hypothesized that children and adults would be equal with regard to tDCS safety, tolerability, and acceptability. We tested this hypothesis using a Bayesian approach. Sixty participants aged 6–45 (balanced for sex) participated in a randomized double-blind controlled trial. They were randomly assigned to two ten-minute tDCS sessions with varying amperages and electrode locations. The primary outcome measure of this study was the intensity of 13 potential side effects evaluated at six different time points spanning two weeks. Independent sample Bayes factor tests were conducted between children/adults, males/females, clinical/healthy, and low/high amperage groups. As predicted, there was moderate support for the null hypothesis in all between-group analyses. There were no serious adverse events or dropouts, and the number needed to treat for an additional harmful outcome was 23. This study provided evidence supporting the overall short-term safety, tolerability, and acceptability of tDCS including amperages up to 2 mA and different electrode placements.
Cardiopulmonary Profiling of Athletes with Post-Exertional Malaise after COVID-19 Infection—A Single-Center Experience
Authors: Wernhart Simon , Weihe Eberhard , Totzeck Matthias , Balcer Bastian , Rassaf Tienush , Luedike Peter
Published: June 2023

(1) Background: Cardiopulmonary exercise testing (CPET) has been suggested by the European Society of Cardiology (ESC) for assessing the exercise limitations of apparently healthy individuals, but data on elite athletes regarding this test are scarce. (2) Methods: We analyzed CPET in elite (n = 43, 21.9 ± 3.7 years) and recreational (n = 40, 34.7 ± 13.0 years) athletes with persistent subjective exercise intolerance and post-exertional malaise (PEM) after COVID-19 infection. The primary outcome was the point prevalence of the adequate cardiopulmonary response (ACPR), defined by the presence of all of the following ESC criteria for apparently healthy individuals: (1) >100% of predicted peak oxygen consumption (predVO2peak), (2) VE/VCO2 < 30, (3) no exercise oscillatory ventilation (EOV), and (4) heart rate recovery of ≥12 beats/minute 1 min after exercise termination (HRR1). Results: ACPR occurred more frequently in elite athletes than in recreational athletes (70.0% vs. 39.5%; p = 0.005), mainly driven by the lower VE/VCO2 (<30: 97.7% vs. 65%, p < 0.001). Elite (11.6%) and recreational athletes (22.5%) showing a plateau of O2 pulse did not display ACPR. Conclusions: ACPR was not observed in all recreational and elite athletes with PEM. In particular, perturbed VE/VCO2 and the plateauing of O2 pulse are suitable for quantifying exercise limitations and may identify a high-risk population with long-COVID-19 syndrome who require their training intensities to be adapted.
Infliximab for the Treatment of Inflammatory Labyrinthitis: A Retrospective Cohort Study
Authors: Djian Cassandre , Champion Karine , Lai Nicolas , Drouet Ludovic , Amador Borrero Blanca , Depond Audrey , Mouly Stéphane , Jourdaine Clément , Herman Philippe , Eliezer Michael , Hautefort Charlotte , Sène Damien
Published: June 2023

Inflammatory labyrinthitis is defined as a fluctuant vestibulo-cochlear syndrome associated with an impairment of the blood-labyrinthine barrier (BLB) on delayed FLAIR MRI sequences. Systemic and intratympanic corticosteroids are the gold standard treatment but their effect is frequently insufficient. The objective is here to determine whether infliximab could be of value in the treatment of bilateral inflammatory labyrinthitis. A retrospective monocentric study was conducted between January 2013 and December 2021. All patients included in the study were affected with a bilateral vestibulo-cochlear syndrome associated with bilateral blood-labyrinthine barrier impairment. Patients were administered infliximab at the dose of 5 mg/kg every 6 weeks for 6 months. Audiometry, MRI with delayed FLAIR sequences on the labyrinth, and corticosteroid doses still required were assessed both before and after treatment with infliximab was completed. Pure-tone average (PTA) was the primary outcome. The secondary outcomes were the speech recognition threshold (SRT), the Dizziness Handicap Inventory (DHI) score, and the corticosteroid (CS) dose. A total of nine patients including five men and four women were enrolled in the study. Thirteen ears were analyzed. After a 6-month period of treatment, the mean PTA (54 ± 24 db versus 66 ± 22 db; p = 0.027), SRT (54 ± 37 db versus 66 ± 32 db; p = 0.041) and DHI score (27 ± 15 versus 9 ± 2; p = 0.032) significantly improved. After the 6-month treatment period, the mean CS dose decreased from 38 ± 33 to 6 ± 5 mg/day (p = 0.003). We conclude that infliximab substantially improves the vestibulo-cochlear function in patients with bilateral inflammatory labyrinthitis and could be of value in corticosteroid-dependent cases.
Pain Management at the End of Life in the Emergency Department: A Narrative Review of the Literature and a Practical Clinical Approach
Authors: Serra Sossio , Spampinato Michele Domenico , Riccardi Alessandro , Guarino Mario , Fabbri Andrea , Orsi Luciano , De Iaco Fabio De
Published: June 2023

Access to pain management is a fundamental human right for all people, including those who are at the end of life (EOL). In end-stage patients, severe and uncontrolled pain is a common cause of admission to the emergency department (ED), and its treatment is challenging due to its complex, often multifactorial genesis. The aim of this narrative review was to identify the available literature on the management of severe EOL pain in the ED. The MEDLINE, SCOPUS, EMBASE, and CENTRAL databases were searched from inception to 1 April 2023 including randomised controlled trials, observational studies, systemic or narrative reviews, case reports, and guidelines on the management of EOL pain in the ED. A total of 532 articles were identified, and 9 articles were included (5 narrative reviews, 2 retrospective studies, and 2 prospective studies). Included studies were heterogeneous on the scales used and recommended for pain assessment and the recommended treatments. No study provided evidence for a better approach for EOL patients with pain in the ED. We provide a narrative summary of the findings and a review of the management of EOL pain in clinical practice, including (i) the identification of the EOL patients and unmet palliative care needs, (ii) a multidimensional, patient-centred assessment of the type and severity of pain, (iii) a multidisciplinary approach to the management of end-of-life pain, including an overview of non-pharmacological and pharmacological techniques; and (iv) the management of special situations, including rapid acute deterioration of chronic pain, breakthrough pain, and sedative palliation.
Helicobacter pylori Diagnostic Tests Used in Europe: Results of over 34,000 Patients from the European Registry on Helicobacter pylori Management
Authors: García-Morales Natalia , Pérez-Aísa Ángeles , Fiorini Giulia , Tepes Bojan , Castro-Fernández Manuel , Lucendo Alfredo , Voynovan Irina , Bujanda Luis , Garre Ana , Rodrigo Luis , Martínez Domínguez Samuel Jesús , Denkovski Maja , Huguet Malavés Jose M. , Jonaitis Laimas , Bumane Renate , Zaytsev Oleg , Mata Romero Pilar , Barrio Jesús , Fernández-Salazar Luis , Sarsenbaeva Aiman Silkanovna , Ortiz Polo Inmaculada , Alekseenko Sergey , Saracino Ilaria Maria , Vaira Dino , Keco-Huerga Alma , Bordin Dmitry , Gasbarrini Antonio , Lerang Frode , Rokkas Theodore , Kupčinskas Juozas , Leja Marcis , Babayeva Gulustan , Marcos Pinto Ricardo , Tonkić Ante , Smith Sinead , Phull Perminder , Buzas Gyorgy M. , Simsek Halis , Boltin Doron , Gridnyev Oleksiy , Venerito Marino , Milivojevic Vladimir , Torà Núria , Cano-Català Anna , Moreira Leticia , Nyssen Olga P. , Mégraud Francis , O’Morain Colm , Gisbert Javier P. , Puig Ignasi ,   on behalf of Hp-EuReg Investigators
Published: June 2023

Background and aims: Several methods are available to diagnose Helicobacter pylori infection. Our objective was to evaluate the tests used for both the initial diagnosis and the confirmation of eradication after treatment in Europe. Methods: The European Registry on the management of Helicobacter pylori infection is an international, multicentre, prospective, non-interventional registry aiming to evaluate the management of Helicobacter pylori-infected patients in Europe. Countries with at least 100 cases registered from June 2013 to April 2021, and with a validated diagnostic method were analysed. Data were quality reviewed. Results: A total of 34,920 adult patients from 20 countries were included (mean age 51 years; 61% women). To establish the initial diagnosis, invasive tests were performed in 19,801 (71%) patients, non-invasive in 11,369 (41%), and both in 3437 (12%). The most frequent were histology (n = 11,885; 43%), a rapid urease test (n = 10,636; 38%) and an urea breath test (n = 7577; 27%). According to the age, invasive tests were indicated in 11,179 (77%) ≥50 years, and in 8603 (65%) <50 years. Depending on the country, the use of invasive tests ranged from 29–99% in <50 years to 60–99% in ≥50. Most of the tests used to confirm eradication were non-invasive (n = 32,540; 93%), with the urea breath test being the most frequent (n = 32,540; 78%). In 2983 (9%) post-treatment tests, histology (n = 1887; 5%) or a rapid urease test (n = 1223; 4%) were performed. Conclusion: A great heterogeneity was observed for the initial diagnosis and confirmation of the eradication. The reasons for the apparent lack of adherence to the clinical guidelines should be further explored.
Elective Endovascular Aneurysm Repair (EVAR) for the Treatment of Infrarenal Abdominal Aortic Aneurysms of 5.0–5.5 cm: Differences between Men and Women
Authors: Martinelli Ombretta , Cuozzo Simone , Miceli Francesca , Gattuso Roberto , D’Andrea Vito , Sapienza Paolo , Bellini Maria Irene
Published: June 2023

Background: There is significant debate regarding the existence of sex-related differences in the presentation, treatment, and outcomes of men versus women affected by abdominal aortic aneurysm (AAA). The purpose of this study is to compare endovascular aneurysm repair (EVAR) of infrarenal AAAs with the current sex-neutral 5.0–5.5 cm-diameter threshold for intervention between the two sexes. Methods: Retrospective review of consecutive cases from a single teaching institution over a period of five years of patients who had undergone elective EVAR for AAAs between 5.0 and 5.5 cm in diameter. Outcomes of interest were compared according to sex. Results: Ninety-four patients were included in the analysis, with a higher prevalence of men (53%). Females were older at the time of repair, 78 ± 5.1 years, versus 71.7 ± 7 years (p < 0.01), and had higher incidence of underlying comorbidities, namely, arrhythmia, chronic kidney disease, and previous carotid revascularization. Women had higher incidence of immediate systemic complications (p = 0.021), post-operative AMI (p = 0.001), arrhythmia (p = 0.006), pulmonary oedema (p < 0.001), and persistent renal dysfunction (p = 0.029). Multivariate analysis for post-operative factors associated to mortality and adjusted for sex confirmed that AMI (p = 0.015), arrhythmia (p = 0.049), pulmonary oedema (p = 0.015), persistent renal dysfunction (p < 0.001), cerebral ischemia (p < 0.001), arterial embolism of lower limbs (p < 0.001), and deep-vein thrombosis of lower limbs (p < 0.001) were associated to higher EVAR-related mortality; a higher incidence of post-operative AMI (p = 0.014), pulmonary edema (p = 0.034), and arterial embolism of lower limbs (p = 0.046) were associated to higher 30-days mortality. In females there was also a higher rate of suprarenal fixation (p = 0.026), insertion outside the instruction for use (p = 0.035), and a more hostile neck anatomy with different proximal aortic diameter (p < 0.001) and angle (p = 0.003). Conclusions: A similar threshold of size of AAA for elective surgery for both males and females might not be appropriate for surgical intervention, as females tend to have worse outcomes. Further population-based studies are needed to guide on sex-related differences and intervention on AAA.
Temporal Changes in Clinical Outcomes after Minimally Invasive Surgery for Hallux Valgus Correction in Women without Postoperative Complications
Authors: Motta Luci M. , Manchado Ignacio , Blanco Gustavo , Quintana-Montesdeoca María P. , Garcés Laura , Garcés Gerardo L.
Published: June 2023

Minimally invasive surgery (MIS) is currently used to correct hallux valgus deformities. Most studies reporting on MIS techniques to correct hallux valgus deformities included patients with postoperative complications. These reported complications, with an average rate of 23%, had significant negative effects on the clinical outcomes in this patient population. In the present study, a cohort of 63 women who underwent MIS hallux valgus correction was assessed preoperatively and at a mean follow-up of 1.0, 4.7, and 6.5 years using the American Orthopaedic Foot and Ankle Society (AOFAS) scale and the Manchester Oxford Foot Questionnaire (MOXFQ). The main criterion for inclusion in this cohort was a lack of complications during the entire follow-up period. The results showed significant improvements in both AOFAS and MOXFQ scores between the preoperative and 1-year follow-up assessments. By contrast, clinically small and nonsignificant changes were observed among postoperative follow-up values. The number of enrolled patients needs to be increased in future studies, with different surgeons and techniques included. Nevertheless, our study findings will inform patients about the outcomes they can expect over the years if no complications occur.
Sediment Transport of Coastal Region Using Time-Series Unmanned Aerial Vehicle Spatial Data
Authors: Kim Sulki , Chang Sungyeol , Shin Sungwon , Do Kideok , Kim Inho
Published: June 2023

Continuous monitoring of the varying topographical characteristics of shorelines is important for effective coastal management. Closed-circuit television (CCTV) cameras are installed to accumulate photographic data on coastal topographical changes. The overall change in the coastal waters can be intuitively understood from the images. However, the amount of three-dimensional (3D) changes that can be grasped is limited. To address this, studies have employed aerial photogrammetry, which is the use of unmanned aerial vehicles (UAVs) to capture aerial pictures, construct 3D models of target areas, and perform analysis through scale-invariant feature transform and structure from motion technologies. Although highly efficient, this technique requires several ground-control points (GCPs), which could corrupt the overall imagery. This study designs real-time kinematics—global navigation satellite system (RTK–GNSS) UAV, which requires few GCPs. To evaluate the positional accuracy of the captured UAV orthographic images and digital surface models (DSMs) used for precise coastal terrain measurements, a virtual reference service survey was performed to determine the vertical errors. The R-squared was 0.985, which is close to 1.0. Short-term and one-year topographic changes before and after a storm were investigated using time-series UAV image data after a coastal maintenance project. Analysis of the coefficient of variation in the beach volume for one year revealed that submerged breakwater reduced erosion during high wave resistance. The submerged breakwater located in the center exhibited variability similar to the opening. Hence, this method is more suitable for periodically monitoring coastal areas.
Micro/Bubble Drag Reduction Focused on New Applications
Authors: García-Magariño Adelaida , Lopez-Gavilan Pablo , Sor Suthyvann , Terroba Félix
Published: June 2023

Bubble drag reduction has been shown to be a promising technique for reducing the drag in ships, thus reducing the emission of pollutants and allowing the compliance with the new requirements imposed recently in this respect. Different searches have been conducted in the publications related to this technique, and an increase in interest has been shown, especially in the last decade. In this context, a review of the experimental work related to bubble drag reduction published in the last decade is presented in the present article. The works were classified according to the facility used (towing tank, cavitation tunnel, water channel, Taylor–Couette…), and the main finding are presented. It was found that two new trends in research have arisen, while there are still contradictions in the fundamental basis, which needs further study.
Microbial Biofilm Colonizing Plastic Substrates in the Ross Sea (Antarctica): First Overview of Community-Level Physiological Profiles
Authors: Caruso Gabriella , Maimone Giovanna , Rappazzo Alessandro Ciro , Dell’Acqua Ombretta , Laganà Pasqualina , Azzaro Maurizio
Published: June 2023

The microbial colonization of plastic substrates made of polyvinylchloride (PVC) and polyethylene (PE) was studied in Tethys and Road Bays (Ross Sea, Antarctica) in order to evaluate the metabolic profiles of the plastisphere community in comparison with those of the surrounding waters. PVC and PE panels, mounted on stainless steel structures, were deployed in the austral summer 2017 at 5 and 20 m and recovered one year later at four different stations (Amorphous Glacier-AG was potentially impacted by the ice-melting process, and its control site was within Tethys Bay-TB; Road Bay-RB, close to the wastewater plant of the Italian research station Mario Zucchelli and its control site Punta Stocchino-PTS). Additional panels were settled in Road Bay at 5 m and recovered after three months to follow time variability in the microbial colonization process. At the same times and depths as plastic substrates, water samples were also collected. Carbon substrates’ utilization rates were determined on scraped microbial biofilm and water samples, with a fluorimetric assay based on 96-well Biolog Ecoplates. Complex carbon sources, carbohydrate and amines were the organic substrates that mostly fuelled the community metabolism in the RB area, while in the TB area, in addition to carbohydrates, phosphate carbon compounds and amino acids were also actively utilized. Within Road Bay, small differences in the physiological profiles were found, with higher metabolic rates in the biofilm community after 3 months’ deployment (late austral summer period) compared to 12 months, suggesting that autumn to spring period conditions negatively affected foulers’ metabolism. Moreover, different metabolic profiles between the plastisphere and the pelagic microbial community were observed; this last utilized a higher number of carbon sources, while plastic substrates were colonized by a more specialized community. Higher carbon substrate utilization rates were recorded at RB and AG stations, receiving organic supply from anthropic activity or ice melting sources, respectively, compared to their control sites. These results highlighted the functional plasticity of the microbial community, with the adaptive ability to utilize a diversified range of organic substrates.
Analytical Calculation of Instantaneous Liquefaction of a Seabed around Buried Pipelines Induced by Cnoidal Waves
Authors: Fu Changjing , Wang Jinguo , Zhao Tianlong
Published: June 2023

Cnoidal wave theory perfectly describes nearshore wave characteristics. However, cnoidal wave theory is not widely applied in practical engineering because the formula for the wave profile involves a complex Jacobian elliptic function. In this paper, the approximate cnoidal wave theory is presented. Based on the Biot consolidation theory and the approximate cnoidal wave theory, an analytical solution for the pore water pressure around buried pipelines caused by waves is derived. In addition, based on the principle of effective stress, a theory of soil liquefaction around pipelines is proposed. The theoretical results were virtually identical to the results obtained in a practical flume test. Thus, the analytical method proposed in this paper is feasible. Further, the theory is applied to analyze the instantaneous liquefaction of the seabed around buried pipelines and the stability of the pipeline in the Chengdao oilfield.
Autonomous Obstacle Avoidance in Crowded Ocean Environment Based on COLREGs and POND
Authors: Peng Xiao , Han Fenglei , Xia Guihua , Zhao Wangyuan , Zhao Yiming
Published: June 2023

In crowded waters with unknown obstacle motion information, traditional methods often fail to ensure safe and autonomous collision avoidance. To address the challenges of information acquisition and decision delay, this study proposes an optimized autonomous navigation strategy that combines deep reinforcement learning with internal and external rewards. By incorporating random network distillation (RND) with proximal policy optimization (PPO), the interest of autonomous ships in exploring unknown environments is enhanced. Additionally, the proposed approach enables the autonomous generation of intrinsic reward signals for actions. For multi-ship collision avoidance scenarios, an environmental reward is designed based on the International Regulations for Preventing Collision at Sea (COLREGs). This reward system categorizes dynamic obstacles into four collision avoidance situations. The experimental results demonstrate that the proposed algorithm outperforms the popular PPO algorithm by achieving more efficient and safe collision avoidance decision-making in crowded ocean environments with unknown motion information. This research provides a theoretical foundation and serves as a methodological reference for the route deployment of autonomous ships.
Isolation of Terbinafine-Resistant Trichophyton rubrum from Onychomycosis Patients Who Failed Treatment at an Academic Center in New York, United States
Authors: Hwang Jonathan K. , Bakotic Wayne L. , Gold Jeremy A. W. , Magro Cynthia M. , Lipner Shari R.
Published: June 2023

Onychomycosis is a common nail infection. Terbinafine-resistant dermatophyte infections pose an emerging global public health concern, but few cases have been described in the United States. We retrospectively reviewed and characterized clinical, histopathological, and mycological features of patients with mycologically confirmed onychomycosis who failed oral terbinafine treatment for onychomycosis at a U.S. academic nail referral center and ascertained for terbinafine-resistant isolates. During 1 June 2022–31 January 2023 at Weill Cornell Medicine in New York City, USA, 96 patients with mycologically confirmed onychomycosis were treated with oral terbinafine. Among 64 patients with adequate follow-up, 36 had clinical or complete cure. Of 28 patients who failed treatment, 17 underwent terbinafine resistance testing. Trichophyton rubrum with terbinafine resistance-conferring mutations was isolated from two patients. Overall, terbinafine failures for onychomycosis were relatively common, with some cases associated with terbinafine-resistant T. rubrum infections. These findings underscore the need for a clinical awareness of this emerging problem and public health efforts to monitor and prevent spread. We highlight the importance of diagnostic testing and species identification for onychomycosis patients and the increasingly important role of fungal identification and susceptibility testing to guide therapy.
Genomic Characterization and Functional Description of Beauveria bassiana Isolates from Latin America
Authors: Solano-González Stefany , Castro-Vásquez Ruth , Molina-Bravo Ramón
Published: June 2023

Beauveria bassiana is an entomopathogenic fungus used in agriculture as a biological controller worldwide. Despite being a well-studied organism, there are no genomic studies of B. bassiana isolates from Central American and Caribbean countries. This work characterized the functional potential of eight Neotropical isolates and provided an overview of their genomic characteristics, targeting genes associated with pathogenicity, the production of secondary metabolites, and the identification of CAZYmes as tools for future biotechnological applications. In addition, a comparison between these isolates and reference genomes was performed. Differences were observed according to geographical location and the lineages of the B. bassiana complex to which each isolate belonged.
Arterial Blood Gas Analysis for Survival Prediction in Pediatric Patients with Out-of-Hospital Cardiac Arrest
Authors: Lee Sang-Hwan , Shin Hyungoo , Cho Yongil , Oh Jaehoon , Choi Hyuk-Joong ,   The Korean Cardiac Arrest Research Consortium (KoCARC) Investigators
Published: June 2023

Arterial blood gas analysis (ABGA) is one of the few tests performed during cardiopulmonary resuscitation (CPR). There have been some studies on the prediction of survival outcomes in adult out-of-hospital cardiac arrest (OHCA) patients during CPR using ABGA results. However, in pediatric OHCA patients, the prognosis of survival outcome based on ABGA results during CPR remains unclear. We retrospectively analyzed prospectively collected data from the Korean Cardiac Arrest Resuscitation Consortium (KoCARC) registry, a multicenter OHCA registry of Republic of Korea. We analyzed 108 pediatric (age < 19 years) OHCA patients between October 2015 and June 2022. Using multivariable logistic regression, an adjusted odds ratio (aOR) was obtained to validate the ABGA results of survival to hospital admission and survival to discharge. The variables associated with survival to hospital admission were non-comorbidities (aOR 3.03, 95% confidence interval (CI) 1.22–7.53, p = 0.017) and PaO2 > 45.750 mmHg (aOR 2.69, 95% CI 1.13–6.42, p = 0.026). There was no variable that was statistically significant association with survival to discharge. PaO2 > 47.750 mmHg and non-comorbidities may serve as an independent prognostic factor for survival to hospital admission in pediatric OHCA patients. However, the number of cases analyzed in our study was relatively small, and there have been few studies investigating the association between ABGA results during CPR and the survival outcome of pediatric OHCA patients. Therefore, further large-scale studies are needed.
Development and Validation of Risk Prediction Models for Colorectal Cancer in Patients with Symptoms
Authors: Xu Wei , Mesa-Eguiagaray Ines , Kirkpatrick Theresa , Devlin Jennifer , Brogan Stephanie , Turner Patricia , Macdonald Chloe , Thornton Michelle , Zhang Xiaomeng , He Yazhou , Li Xue , Timofeeva Maria , Farrington Susan , Din Farhat , Dunlop Malcolm , Theodoratou Evropi
Published: June 2023

We aimed to develop and validate prediction models incorporating demographics, clinical features, and a weighted genetic risk score (wGRS) for individual prediction of colorectal cancer (CRC) risk in patients with gastroenterological symptoms. Prediction models were developed with internal validation [CRC Cases: n = 1686/Controls: n = 963]. Candidate predictors included age, sex, BMI, wGRS, family history, and symptoms (changes in bowel habits, rectal bleeding, weight loss, anaemia, abdominal pain). The baseline model included all the non-genetic predictors. Models A (baseline model + wGRS) and B (baseline model) were developed based on LASSO regression to select predictors. Models C (baseline model + wGRS) and D (baseline model) were built using all variables. Models’ calibration and discrimination were evaluated through the Hosmer-Lemeshow test (calibration curves were plotted) and C-statistics (corrected based on 1000 bootstrapping). The models’ prediction performance was: model A (corrected C-statistic = 0.765); model B (corrected C-statistic = 0.753); model C (corrected C-statistic = 0.764); and model D (corrected C-statistic = 0.752). Models A and C, that integrated wGRS with demographic and clinical predictors, had a statistically significant improved prediction performance. Our findings suggest that future application of genetic predictors holds significant promise, which could enhance CRC risk prediction. Therefore, further investigation through model external validation and clinical impact is merited.
Global Anti-Money Laundering and Combating Terrorism Financing Regulatory Framework: A Critique
Authors: Gaviyau William , Sibindi Athenia Bongani
Published: June 2023

Money launderers prefer to use financial services as the ideal medium for laundering. This study aimed to provide an overview of the global AML/CFT regulations, application and how they should evolve in this dynamic environment. To gather more insight, a qualitative study was undertaken with relevant documents analysed. The main finding was that country implementation of the global AML/CFT regulations differed due to political and economic factors, amongst others. While the various AML/CFT enforcements done by sampled countries were mainly cease and desist orders and monetary penalties that were publicised, the drawbacks of global AML/CFT regulations centred on the application of these regulations and emerging trends. These include, among other definitions of money laundering, reference to the three stages of money laundering, the link between penalty and violations, technological innovations and regulation paradigm shift, cyber-attacks, and data privacy. This study contributes to the application and growing body of knowledge in that the advent of technology has resulted in better consumer experiences, new payment platforms, products and services. However, these innovations have broadened emerging money laundering risks and risks to the financial system in general. Hence, there is a need to conduct research-based FATF recommendations, as risk is dynamic and not static.
Impact of Urbanization on Ecosystem Service Value from the Perspective of Spatio-Temporal Heterogeneity: A Case Study from the Yellow River Basin
Authors: Cheng Yonghui , Kang Qi , Liu Kewei , Cui Peng , Zhao Kaixu , Li Jianwei , Ma Xue , Ni Qingsong
Published: June 2023

Ecosystem services are the beneficial goods and services that ecosystems provide to humans. Urbanization is an important feature of human social development. While promoting economic and social development, it also brings about land degradation, resource depletion, environmental pollution and other problems, intensifying the transformation of natural ecosystems into semi-natural and artificial ecosystems, ultimately leading to the loss of ecosystem service functions and declining value. The study of the impact of urbanization on the value of ecosystem services is of critical importance for the conservation of ecosystems and sustainable development. This study examined the spatio-temporal patterns of urbanization’s impacts on ecosystem service value in the Yellow River Basin from the perspective of spatio-temporal heterogeneity. Findings: (1) Both the ecosystem service value (ESV) and urbanization level (UL) in the Yellow River Basin were on the rise on the whole, but they were significantly spatially negatively correlated and mainly characterized by the high–low spatial clustering of “low ESV–high UL” and “high ESV–low UL”. This negative correlation was gradually weakened with the transformation of the urbanization development mode and ecological restoration projects in the Yellow River Basin. (2) The impacts of the five urbanization subsystems on the value of ecosystem services were diverse. Landscape urbanization had a negative impact on the value of ecosystem services in all regions; economic urbanization and innovation urbanization changed from having a negative to a positive impact; and demographic urbanization and social urbanization had both a positive and a negative impact. (3) To promote the coordinated development of ecological environmental protection and urbanization in the YRB, this paper proposes to change the urbanization development model, implement ecological restoration by zoning, and formulate classified development plans. This study compensates for the shortcomings of current studies that ignore the different impacts of urbanization subsystems on ecosystem service value and lack sufficient consideration of the spatio-temporal heterogeneity characteristics of urbanization and ESVs, enriches the theoretical understanding of the interrelationships between natural and human systems in basin areas, and provides a scientific basis for the rational formulation of urban planning and ecological protection policies in the region, which is of great theoretical and practical significance.
Cultivation and Nitrogen Management Practices Effect on Soil Carbon Fractions, Greenhouse Gas Emissions, and Maize Production under Dry-Land Farming System
Authors: Ren Honglei , Xu Shengjun , Zhang Fengyi , Sun Mingming , Zhang Ruiping
Published: June 2023

Effective nitrogen management practices by using two cultivation techniques can improve corn productivity and soil carbon components such as soil carbon storage, microbial biomass carbon (MBC), carbon management index (CMI), and water-soluble carbon (WSC). It is essential to ensure the long-term protection of dry-land agricultural systems. However, excessive application of nitrogen fertilizer reduces the efficiency of nitrogen use and also leads to increased greenhouse gas emissions from farming soil and several other ecological problems. Therefore, we conducted field trials under two planting methods during 2019–2020: P: plastic mulching ridges; F: traditional flat planting with nitrogen management practices, i.e., 0: no nitrogen fertilizer; FN: a common nitrogen fertilizer rate for farmers of 290 kg ha−1; ON: optimal nitrogen application rate of 230 kg ha−1; ON75%+DCD: 25% reduction in optimal nitrogen fertilizer rate + dicyandiamide; ON75%+NC: 25% reduction in optimal nitrogen rate + nano-carbon. The results showed that compared to other treatments, the PON75%+DCD treatment significantly increased soil water storage, water use efficiency (WUE), and nitrogen use efficiency (NUE) because total evapotranspiration (ET) and GHG were reduced. Under the PON75%+DCD or PON75%+NC, the soil carbon storage significantly (50% or 47%) increased. The PON75%+DCD treatment is more effective in improving MBC, CMI, and WSC, although it increases gaseous carbon emissions more than all other treatments. Compared with FFN, under the PON75%+DCD treatment, the overall CH4, N2O, and CO2 emissions are all reduced. Under the PON75%+DCD treatment, the area scale GWP (52.7%), yield scale GWP (90.3%), biomass yield (22.7%), WUE (42.6%), NUE (80.0%), and grain yield (32.1%) significantly increased compared with FFN, which might offset the negative ecological impacts connected with climate change. The PON75%+DCD treatment can have obvious benefits in terms of increasing yield and reducing emissions. It can be recommended to ensure future food security and optimal planting and nitrogen management practices in response to climate change.
Detecting Long-Term Series Eco-Environmental Quality Changes and Driving Factors Using the Remote Sensing Ecological Index with Salinity Adaptability (RSEISI): A Case Study in the Tarim River Basin, China
Authors: Chen Wen , Wang Jinjie , Ding Jianli , Ge Xiangyu , Han Lijing , Qin Shaofeng
Published: June 2023

Ecological challenges resulting from soil salinization in the Tarim River Basin (TRB), exacerbated by climate change and human activities, have emphasized the need for a quick and accurate assessment of regional ecological environmental quality (EEQ) and driving mechanisms. To address this issue, this study has developed a remote-sensing ecological index with salinity adaptability (RSEISI) for EEQ assessment in the Tarim River Basin by integrating the comprehensive salinity index (CSI) into the remote-sensing ecological index (RSEI). The RSEISI enhances the sensitivity of soil salinity and characterizes the surface features of arid regions, thus expanding the applicability. Then, we used time-series analysis methods and a geodetector to quantify the spatial temporal trends and driving factors of EEQ in the TRB from 2000 to 2022. The results show that the RSEISI with salinity adaptation effectively monitors the EEQ of the TRB. The EEQ of the TRB displayed the situation of oasis expansion, desert deterioration, and glacier melting, and the multiyear average EEQ grades were dominated by medium and poor grades in desert and saline areas, while medium, good, and excellent grades were concentrated in oasis and mountainous areas. Looking at the trend of change in conjunction with land-use types, the EEQ of the TRB showed a mild degradation trend mainly in unused land, followed by a mild improvement trend in cropland and grassland. The Hurst index indicated that the EEQ of most areas of the TRB will improve in the future. Soil type, land use, precipitation, and temperature were considered to be key factors affecting the EEQ across the TRB, and changes in the EEQ were found to be the interaction of multiple factors. This study may provide innovative concepts and methodologies, scientific and technological support for ecological management, and green development models in the northwest arid zone.
Pauses and Parsing: Testing the Role of Prosodic Chunking in Sentence Processing
Authors: Harrington Stack Caoimhe , Watson Duane G.
Published: June 2023

It is broadly accepted that the prosody of a sentence can influence sentence processing by providing the listener information about the syntax of the sentence. It is less clear what the mechanism is that underlies the transmission of this information. In this paper, we test whether the influence of the prosodic structure on parsing is a result of perceptual breaks such as pauses or whether it is the result of more abstract prosodic elements, such as intonational phrases. In three experiments, we test whether different types of perceptual breaks, e.g., intonational boundaries (Experiment 1), an artificial buzzing sound (Experiment 2), and an isolated pause (Experiment 3), influence syntactic attachment in ambiguous sentences. We find that although full intonational boundaries influence syntactic disambiguation, the artificial buzz and isolated pause do not. These data rule out theories that argue that perceptual breaks indirectly influence grammatical attachment through memory mechanisms, and instead, show that listeners use prosodic breaks themselves as cues to parsing.
The Renin–Angiotensin System in COVID-19: Can Long COVID Be Predicted?
Authors: König Simone , Vollenberg Richard , Tepasse Phil-Robin
Published: June 2023

(1) Background: Co-morbidities such as hypertension and cardiovascular disease are major risk factors for severe COVID-19. The renin–angiotensin system (RAS) is critically involved in their pathophysiology and is counter-balanced by both angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV-2, and the kallikrein–kinin system (KKS). Considerable research interest with respect to COVID-19 treatment is currently being directed towards the components of these systems. In earlier studies, we noticed significantly reduced carboxypeptidase N (CPN, KKS member) activity and excessive angiotensin-converting enzyme (ACE, RAS member) activity in the sera of both hospitalized COVID-19 patients and a subgroup of convalescent patients. The data had been obtained using labeled bradykinin (BK) as a reporter peptide, which is a target of both CPN and ACE. The data were supplemented with mass-spectrometry-based serum proteomic analysis. Here, we hypothesize that the degree of BK serum degradation could be indicative of Long COVID. (2) Review and Discussion: The recent literature is briefly reviewed. The fact that the levels of the BK serum degradation products did not reach normal concentrations in almost half of the patients during convalescences could have been partially due to a dysregulated RAS. (3) Conclusions: Standard tests for routine patient care in Long COVID come often back normal. We suggest that the measurement of selected members of the RAS such as ACE and angiotensin II or the use of our BK degradation assay could identify Long COVID candidates. Clinical studies are required to test this hypothesis.
Therapeutic Implications of Probiotics in the Gut Microbe-Modulated Neuroinflammation and Progression of Alzheimer’s Disease
Authors: Murai Toshiyuki , Matsuda Satoru
Published: June 2023

Alzheimer’s disease (AD) is characterized by the accumulation of specific proteins in the brain. A recent study revealed that manipulating gut microbiota (GM) significantly reduced tau pathology and neurodegeneration in an apolipoprotein E isoform-dependent manner. The resilience of a healthy microbiota protects it from a variety of dysbiosis-related pathologies. Convincing evidence has demonstrated the roles of GM in the pathogenesis of AD, which are partly mediated by modified microglial activity in the brain. Therefore, modulation of GM may be a promising therapeutic option for AD prevention. In addition to providing the cells with energy and affecting microglial maturation, these microbial metabolites appear to influence neuronal function. One of the potential therapeutic approaches targeting GM may involve using probiotics. Additionally, human GM and its metabolites have also become potential therapeutic targets for developing interventions for the prevention of disorders. Synbiotics and postbiotics can also be used to treat AD by modulating GM. In addition, physical activity, exercise, and physical fitness are being considered as potential nonpharmacological therapies to reduce signaling pathways related to neuroinflammation. Therefore, interventions targeting GM might be promising strategies for health promotion.
Simulation-Based Research on Phytoconstituents of Embelia ribes Targeting Proteins with Pathophysiological Implications in Rheumatoid Arthritis
Authors: Radu Andrei-Flavius , Negru Paul Andrei , Radu Ada , Tarce Alexandra Georgiana , Bungau Simona Gabriela , Bogdan Mihaela Alexandra , Tit Delia Mirela , Uivaraseanu Bogdan
Published: June 2023

Rheumatoid arthritis (RA) is a heterogeneous inflammatory disease with an autoimmune origin and an incompletely elucidated pathophysiological mechanism. RA pharmacotherapy is based on chemically or biologically active substances that provide clinical alleviation and remission, but the disease is still incurable. As a result, there remains a need for significant therapeutic development, and adjuvant therapies may play an essential role in the search for novel RA treatment strategies. The aim of the present study was to investigate potential phytocompounds and phytocompound derivates as RA treatment agents, using in silico methodologies. In this regard, five phytoconstituents identified in different structures of Embelia ribes were evaluated by in silico methods for their potential action on target proteins of therapeutic interest in RA. The methodology involved identifying the phytocompound with the highest binding toward the target protein via molecular docking using AutoDock Vina 1.5.7, followed by a ligand-based virtual screening based on the structure of the most promising phytocompound using SwissSimilarity. This process led to the identification of ligands that are not currently utilized in medical practice, but that might have the potential to be used in the management of RA after further extensive experimental endorsements. ZINC000004024651 showed the highest binding affinity for the Bruton’s tyrosine kinase protein, followed by ZINC000000434197 for p38 mitogen-activated protein kinases, ZINC000087606977 for interleukin-1 receptor-associated kinase 4, and ZINC000014728393 for matrix metallopeptidase 9, the latter two showing higher affinity than the co-crystallized compound. The relatively high affinities to target proteins and the pharmacokinetic data obtained by in silico studies using SwisADME suggest a first step for the inclusion of promising new compounds in various more advanced studies, leading to the evaluation of efficacy and safety profiles.
Supplementing Genistein for Breeder Hens Alters the Growth Performance and Intestinal Health of Offspring
Authors: Gao Mingkun , Wang Jiao , Lv Zengpeng
Published: June 2023

Recent research revealed that dietary genistein supplementation for breeder hens can improve the immune function of offspring chicks. However, it remains unknown whether this maternal effect could improve the intestinal health of offspring. This study was conducted to explore the mechanism involved in the maternal effect of genistein on the intestinal mucosa and microbial homeostasis of chicken offspring. A total of 120 Qiling breeder hens were fed a basal diet, a 20 mg/kg genistein-supplemented diet, or a 40 mg/kg genistein-supplemented diet for 4 weeks before collecting their eggs. After hatching, 180 male offspring (60 chickens from each group) were randomly selected and divided into three groups: (1) the offspring of hens fed a basal diet (CON); (2) the offspring of hens fed a low-dose genistein-supplemented diet (LGE); (3) the offspring of hens fed a high-dose genistein-supplemented diet (HGE). At 17 d, 72 male offspring (48 chickens from CON and 24 chickens from LGE) were divided into three groups: (1) the offspring of hens fed a basal diet (CON); (2) the CON group challenged with LPS (LPS); (3) the LGE group challenged with LPS (LPS + LGE). The results showed that maternal genistein supplementation increased the birth weight and serum level of total protein (TP), followed by improved intestinal villus morphology. Continuously, the maternal effect on the body weight of chicks lasted until 21 d. Additionally, it was observed that maternal genistein supplementation exhibited protective effects against LPS-induced morphological damage and intestinal mucosal barrier dysfunction by upregulating the expression of tight junction proteins, specifically ZO-1, Claudin1, E-cadherin, and Occludin, at 21 d. Using 16S rRNA gene sequencing, we demonstrated that maternal supplementation of genistein has the potential to facilitate the maturation of newly hatched chicken offspring by enhancing the abundance of Escherichia coli. Additionally, maternal genistein supplementation can effectively reduce the abundance of Gammaproteobacteria, thus mitigating the risk of bacterial diversity impairment of LPS. In light of these findings, maternal genistein supplementation holds promise as a potential strategy for ameliorating intestinal mucosal damage and modulating the microbiome in chicken offspring.