bluuebunny/crossref_metadata_2025 huggingface dataset:
Can the relationship between overweight/obesity and sleep quality be explained by affect and behaviour?
Authors: Eid S. W. , Brown R. F. , Maloney S. K. , Birmingham C. L.
Published: July 2022

Purpose

Sleep impairment is reported to be a consequence of overweight and obesity. However, the weight–sleep relationship can alternately be explained by demographics (e.g. age) and covariates (i.e. mood/affect and behaviour in overweight/obese people; e.g. night-eating). Thus, we examined the weight–sleep quality relationship after controlling for the effects of affect and common behaviour (i.e. night-eating, insufficient exercise, alcohol and electronic device use).

Methods

Online questionnaires asked 161 overweight, obese or normal-weight participants about their sleep quality, night-eating, physical activity, alcohol use, electronic device use and anxiety and depression at T0 (baseline) and T1 (3 months later). Height and weight and waist and hip circumference were objectively measured at T0 and T1, and physical activity was assessed over 24 h (using actigraphy) at T0 and T1. Hierarchical multiple regression analyses evaluated whether the weight measures (i.e. body-mass-index [BMI], waist-to-hip ratio [WHR] and obesity category [overweight/obese vs. normal-weight]) predicted sleep quality and its components at T0 and T1, after controlling demographics (at step 1) and covariates (affective distress and behaviour) at step 2, and entering weight measures at step 3; maximum 8 variables in the analyses.

Results

High BMI predicted several aspects of sleep quality after taking into account co-existing behaviour, affect and demographics: sleep disturbances at T0 and lower sleep efficiency at T1. WHR and obesity category did not predict any aspects of sleep quality. Several co-existing behaviour were related to or predicted sleep quality score and aspects of sleep quality including night-eating, alcohol use and electronic device use and affective symptoms (i.e. anxiety, depression).

Conclusion

Results suggest that a person’s weight may impact on their sleep quality above and beyond the effects of their co-existing behaviour and affect, although their co-existing behaviour and affect may also adversely impact on sleep quality.

Level of evidence

Level III, evidence obtained from well-designed cohort.
Pro-Social Rule Breaking as a Benchmark of Ethical Intelligence in Socio-Technical Systems
Authors: Ramanayake Rajitha , Nallur Vivek
Published: July 2022

The current mainstream approaches to ethical intelligence in modern socio-technical systems have weaknesses. This paper argues that implementing and validating pro-social rule breaking behaviour can be used as a mechanism to overcome these weaknesses and introduce a sample scenario that can be used to validate this behaviour.
Advanced mid-infrared lightsources above and beyond lasers and their analytical utility
Authors: Hlavatsch Michael , Mizaikoff Boris
Published: July 2022

In the mid-infrared (MIR) spectral range, a series of applications have successfully been shown in the fields of sensing, security and defense, energy conservation, and communications. In particular, rapid and recent developments in MIR light sources have significantly increased the interest in developing MIR optical systems, sensors, and diagnostics especially for chem/bio detection schemes and molecular analytical application scenarios. In addition to the advancements in optoelectronic light sources, and especially quantum and interband cascade lasers (QCLs, ICLs) largely driving the increasing interest in the MIR regime, also thermal emitters and light emitting diodes (LEDs) offer opportunities to alternatively fill current gaps in spectral coverage specifically with analytical applications and chem/bio sensing/diagnostics in the focus. As MIR laser technology has been broadly covered in a variety of articles, the present review aims at summarizing recent developments in MIR non-laser light sources highlighting their analytical utility in the MIR wavelength range.

Graphical abstract
Marriage, bridewealth and power: critical reflections on women's autonomy across settings in Africa
Authors: Akurugu Constance Awinpoka , Dery Isaac , Domanban Paul Bata
Published: July 2022

This article examines ongoing discourses on the importance of the marriage payment and its role in constraining women's autonomy across societies in Africa. First, we review how bridewealth has been conceptualised across multiple disciplines, including the work of evolutionary human scientists. We then summarise our research grounded in residential ethnographic fieldwork data collected over a period of a year in a rural settlement in north-western Ghana. Feminist accounts on women's lived experiences throughout bridewealth practising societies point to their subordination. In some contexts, including northern Ghana, bridewealth is perceived as engendering women's oppression. To liberate women from patriarchal norms, some gender advocates call for undoing of the institution of the marriage payment. Nonetheless, the women who bear the brunt of gendered oppression and the men who derive patriarchal dividends from it are averse to this undoing discourse as the bridewealth normatively secures legitimacy for women. Undoing bridewealth may mean further rendering precarious women's status in the marital family. We conclude that rather than undoing the revered institution of bridewealth, there is need to build on culturally appropriate notions of communitarianism as encapsulated by the Ubuntu philosophy and indigenous systems such as the traditional courts for negotiating the rights of women.
Risk factors for COVID-19-related stress among college-going students
Authors: Bhargav Madhav , Swords Lorraine
Published: July 2022

Objective:

To explore the degree of COVID-19-related stress among college students enrolled in higher level institutions and identify socio-demographic and psychosocial factors that may predict, or be associated with, higher levels of pandemic-related distress.

Method:

Data were obtained from a cross-sectional survey completed by 321 college students primarily recruited from Universities in Ireland. Ages ranged between 18–21 years (n = 176) and 22–25 years (n = 145). Participants answered some demographic questions before completing scales assessing their experience of childhood adversity, their present resilience and levels of psychological distress, as well as their COVID-19-specific stress.

Results:

Multiple regression analysis revealed that students who were female, who had chronic illness, who experienced monetary concerns and who expressed psychological distress experienced greater stress related to COVID-19.

Conclusion:

Findings suggest that certain categories of college students may be at higher risk for experiencing poor mental health during a global pandemic. Higher level institutions should consider this when designing and delivering support services aiming to promote student mental health and alleviate mental distress.
Two-day fasting affects kynurenine pathway with additional modulation of short-term whole-body cooling: a quasi-randomised crossover trial
Authors: Solianik Rima , Schwieler Lilly , Trepci Ada , Erhardt Sophie , Brazaitis Marius
Published: July 2022

Metabolites of the kynurenine (KYN) pathway of tryptophan (TRP) degradation have attracted interest as potential pathophysiological mediators and future diagnostic biomarkers. A greater knowledge of the pathological implications of the metabolites is associated with a need for a better understanding of how the normal behaviour and physiological activities impact their concentrations. This study aimed to investigate whether fasting (FAST) and whole-body cold-water immersion (CWI) affect KYN pathway metabolites. Thirteen young women were randomly assigned to receive the 2-d FAST with two 10-min CWI on separate days (FAST-CWI), 2-d FAST without CWI (FAST-CON), 2-d two CWI on separate days without FAST (CON-CWI) or the 2-d usual diet without CWI (CON-CON) in a randomised crossover fashion. Changes in plasma concentrations of TRP, kynurenic acid (KYNA), 3-hydroxy-kynurenine (3-HK), picolinic acid (PIC), quinolinic acid (QUIN) and nicotinamide (NAA) were determined with ultra-performance liquid chromatography-tandem mass spectrometer. FAST-CWI and FAST-CON lowered TRP concentration (P < 0·05, ηp2 = 0·24), and increased concentrations of KYNA, 3-HK and PIC (P < 0·05, ηp2 = 0·21–0·71) with no additional effects of CWI. The ratio of PIC/QUIN increased after FAST-CWI and FAST-CON trials (P < 0·05) but with a blunted effect in the FAST-CWI trial (P < 0·05) compared with the FAST-CON trials (ηp2 = 0·67). Concentrations of QUIN and NAA were unaltered. This study demonstrated that fasting for 2 d considerably impacts the concentration of several metabolites in the KYN pathway. This should be considered when discussing the potential of KYN pathway metabolites as biomarkers.
ANTHOLOGIA LATINA 109.8 SHB: A NEW READING FOR YOU
Authors: Leary T.J.
Published: July 2022

This note addresses briefly the difficulties associated with the personalities named in the epigram Anth. Lat. 109.8 ShB and their roles before suggesting that tibi should be read rather than mihi in line 8.
Thirsty cities? The supply, management, and perception of water in Byzantine North Africa
Authors: Lamare Nicolas
Published: July 2022

Cities of North Africa experienced a long occupation up to the late 7th c. CE. Despite numerous studies on Late Antique urbanism, no systematic investigation of urban hydraulics has been carried out so far. This paper examines the hydraulic topography of three cities in the Byzantine period (ca. 6th c. CE): Leptis Magna (Tripolitania), Sbeitla (Byzacena), and Timgad (Numidia). This analysis assesses to what extent Late Antique societies managed the cities’ water supply by maintaining or transforming preexisting hydraulic networks. It considers the continuity of aqueducts and the reorganization of water networks, the state of hydraulic management and technology, and the perception of water resources. The hydraulic networks inherited from the Early Roman period were to some extent preserved, although greatly adapted to new concerns for security and new technical and environmental constraints, illustrating the resilience of Late Antique societies.
Old and new evidence for glass in opera sectilia: visual dialogues between appearance and reality
Authors: Gasparini Eleonora
Published: July 2022

This article analyses the phenomenon of glass in wall and floor opera sectilia from the Hellenistic period to Late Antiquity. This type of decoration was developed in Alexandria – as testified by archaeological finds – and then spread across the Greco-Roman world. In Rome the art created a backdrop for a series of displays – especially in imperial palaces and elite housing – that spanned the Imperial era. All the great metropolises were graced by it, including the new capital of the East, Constantinople, where it underwent a renewed flowering. This article analyzes the use of glass material mostly as inserts in marble compositions and, more rarely, in wholly vitreous compositions. It reflects upon the meaning of these different decorative products and attempts to interpret their economic, aesthetic, and symbolic implications.
Welfare Reform and Housing Insecurity: The Impact of Universal Credit Rollout on Demand for Rent Arrears and Homelessness Advice from Citizens Advice in England
Authors: Hardie Iain
Published: July 2022

Universal Credit (UC) has been rolling out since 2013 to radically alter the UK welfare system. Several UC design features, and its changes to benefit generosity, can lead to claimants struggling to afford rent payments. This article uses fixed-effects panel modelling to investigate UC’s housing insecurity impacts within English local authorities (2014 Q1 - 2019 Q1) by bringing together official UC data and Citizens Advice ‘advice trends’ data on rent arrears/homelessness issues within the social/private rented sectors. The results suggest UC rollout is associated with increases in rent arrears advice issues (though not homelessness advice issues). This impact tended to be greater when UC had been rolled out for longer (and therefore reached more claimants), and was greatest in the social rented sector where people are more vulnerable to arrears. This highlights a need to increase the level of UC payments and address its long wait periods and harsh sanctions.
Losses of Tree Cover in California Driven by Increasing Fire Disturbance and Climate Stress
Authors: Wang Jonathan A. , Randerson James T. , Goulden Michael L. , Knight Clarke A. , Battles John J.
Published: July 2022

Forests provide natural climate solutions for sequestering carbon and mitigating climate change, yet are increasingly threatened by increasing temperature and disturbance. Understanding these threats requires accurate information on vegetation dynamics and their drivers, which is currently lacking in many regions experiencing rapid climate change such as California. To address this, we combined remote sensing observations with geospatial databases to develop annual maps of vegetation cover (tree, shrub, and herbaceous) and disturbance type (fire, harvest, and forest die‐off) in California at 30 m resolution from 1985 to 2021. Considering both changes in cover fraction and areal extent, California lost 4,566 km2 of its tree cover area (6.7% relative to initial cover) since 1985. Substantial gains in tree cover area during the 1990s were more than offset by fire‐driven declines since 2000, resulting in greater shrub and herbaceous cover area. Tree cover loss occurred in all ecoregions but was most severe in the southern mountains, where losses from wildfire were not compensated by regrowth in undisturbed areas. Fires and tree cover area loss generally occurred where summer temperatures were greater than 17.5°C, whereas net tree cover gain often occurred in cooler areas, suggesting that ongoing climate warming is threatening forests in many areas. California's vegetation is undergoing rapid transformation, with disturbance rates and climate change posing substantial potential risks to the integrity of California's terrestrial carbon sink.
Insights Into Exhumation and Mantle Hydration Processes at the Deep Galicia Margin From a 3D High‐Resolution Seismic Velocity Model
Authors: Boddupalli Bhargav , Minshull Tim A. , Bayrakci Gaye , Lymer Gaёl , Klaeschen Dirk , Reston Tim J.
Published: July 2022

High‐resolution velocity models developed using full‐waveform inversion (FWI) can image fine details of the nature and structure of the subsurface. Using a 3D FWI velocity model of hyper‐thinned crust at the Deep Galicia Margin (DGM) west of Iberia, we constrain the nature of the crust at this margin by comparing its velocity structure with those in other similar tectonic settings. Velocities representative of both the upper and lower continental crust are present, but there is no clear evidence for distinct upper and lower crustal layers within the hyper‐thinned crust. Our velocity model supports exhumation of the lower crust under the footwalls of fault blocks to accommodate the extension. We used our model to generate a serpentinization map for the uppermost mantle at the DGM, at a depth of 100 ms (∼340 m) below the S‐reflector, a low‐angle detachment that marks the base of the crust at this margin. We find a good alignment between serpentinized areas and the overlying major block bounding faults on our map, suggesting that those faults played an important role in transporting water to the upper mantle. Further, we observe a weak correlation between fault heaves and serpentinization beneath the hanging‐wall blocks, indicating that serpentinization was controlled by complex faulting during rifting. A good match between topographic highs of the S and local highly serpentinized areas of the mantle suggests that the morphology of the S was affected by the volume‐increasing process of serpentinization and deformation of the overlying crust.
The Distribution of Soil Carbon and Nitrogen Stocks Among Dominant Geomorphological Terrain Units in Qarlikturvik Valley, Bylot Island, Arctic Canada
Authors: Ola A. , Fortier D. , Coulombe S. , Comte J. , Domine F.
Published: July 2022

Soils of circumpolar regions store large amounts of carbon (C) and are a crucial part of the global C cycle. Yet, little is known about the distribution of soil C stocks among geomorphological terrain units of glacial valleys in the Arctic. Soil C and nitrogen (N) content for the top 100 cm of the dominant vegetated geomorphological terrain units (i.e., alluvial fans, humid polygons, mesic polygons) at Qarlikturvik Valley, Bylot Island, Canada have been analyzed. Soil C content was greatest in humid low‐center ice‐wedge polygons (82 kg m−2), followed by mesic flat‐center ice‐wedge polygons (40 kg m−2), and alluvial fan area (16 kg m−2), due to prevailing geomorphological processes, differences in vegetation and soil characteristics, as well as permafrost processes. Soil N content was greatest in humid polygons (4 kg m−2), followed by mesic polygons (2 kg m−2), and alluvial fan area (1 kg m−2). Vertically, C and N decreased with increasing depth except for a peak in C at depth in humid polygons, a likely result of past changes in vegetation cover. At Qarlikturvik Valley, which has a size of 121.7 km2, alluvial fans store 0.226 Tg organic C and humid and mesic polygons store 1.643 and 0.218 Tg organic C, respectively in the top 100 cm of soil. Findings like these are important to further constrain pan‐Arctic soil C and N stock estimates and thus climate models.
Thallium Pollution in Europe Over the Twentieth Century Recorded in Alpine Ice: Contributions From Coal Burning and Cement Production
Authors: Legrand Michel , McConnell Joseph R. , Preunkert Susanne , Bergametti Gilles , Chellman Nathan J. , Desboeufs Karine , Plach Andreas , Stohl Andreas , Eckhardt Sabine
Published: July 2022

Emission inventories indicate that thallium, a highly toxic metal, is emitted during coal burning and cement production. These estimates have been established only for the 1980s and 1990s but up to now they have not been compared to long‐term observations. Here we used alpine ice cores to document thallium pollution over Europe since ∼1850. Ice‐core thallium concentrations increased from 1890 to 1910, and decreased after 1965 to concentrations that were half 1890 levels. Comparison of ice‐core trends, estimated past emissions, and state‐of‐the‐art atmospheric aerosol transport modeling suggest that coal burning was responsible for thallium pollution in Europe, particularly from 1920 to 1965 because of high coal consumption at that time. The subsequent decline resulted from decreased coal consumption and reduced emissions following technological improvements. The ice‐core data suggest that the rapid growth of cement production that took place in Europe after 1950 had a limited impact on thallium pollution.
Augmentation of the RNA m6A reader signature is associated with poor survival by enhancing cell proliferation and EMT across cancer types
Authors: Oh Jaeik , Hwa Chanwoong , Jang Dongjun , Shin Seungjae , Lee Soo-Jin , Kim Jiwon , Lee Sang Eun , Jung Hae Rim , Oh Yumi , Jang Giyong , Kwon Obin , An Joon-Yong , Cho Sung-Yup
Published: July 2022

N6-Methyladenosine (m6A) RNA modification plays a critical role in the posttranscriptional regulation of gene expression. Alterations in cellular m6A levels and m6A-related genes have been reported in many cancers, but whether they play oncogenic or tumor-suppressive roles is inconsistent across cancer types. We investigated common features of alterations in m6A modification and m6A-related genes during carcinogenesis by analyzing transcriptome data of 11 solid tumors from The Cancer Genome Atlas database and our in-house gastric cancer cohort. We calculated m6A writer (W), eraser (E), and reader (R) signatures based on corresponding gene expression. Alterations in the W and E signatures varied according to the cancer type, with a strong positive correlation between the W and E signatures in all types. When the patients were divided according to m6A levels estimated by the ratio of the W and E signatures, the prognostic effect of m6A was inconsistent according to the cancer type. The R and especially the R2 signatures (based on the expression of IGF2BPs) were upregulated in all cancers. Patients with a high R2 signature exhibited poor prognosis across types, which was attributed to enrichment of cell cycle- and epithelial–mesenchymal transition-related pathways. Our study demonstrates common features of m6A alterations across cancer types and suggests that targeting m6A R proteins is a promising strategy for cancer treatment.
Dispersion coding of ENZ media via multiple photonic dopants
Authors: Zhou Ziheng , Li Hao , Sun Wangyu , He Yijing , Liberal Iñigo , Engheta Nader , Feng Zhenghe , Li Yue
Published: July 2022

Epsilon-near-zero (ENZ) media are opening up exciting opportunities to observe exotic wave phenomena. In this work, we demonstrate that the ENZ medium comprising multiple dielectric photonic dopants would yield a comb-like dispersion of the effective permeability, with each magnetic resonance dominated by one specific dopant. Furthermore, at multiple frequencies of interest, the resonant supercouplings appearing or not can be controlled discretely via whether corresponding dopants are assigned or not. Importantly, the multiple dopants in the ENZ host at their magnetic resonances are demonstrated to be independent. Based on this platform, the concept of dispersion coding is proposed, where photonic dopants serve as “bits” to program the spectral response of the whole composite medium. As a proof of concept, a compact multi-doped ENZ cavity is fabricated and experimentally characterized, whose transmission spectrum is manifested as a multi-bit reconfigurable frequency comb. The dispersion coding is demonstrated to fuel a batch of innovative applications including dynamically tunable comb-like dispersion profiled filters, radio-frequency identification tags, etc.
FOXM1 regulates glycolysis and energy production in multiple myeloma
Authors: Cheng Yan , Sun Fumou , Thornton Krista , Jing Xuefang , Dong Jing , Yun Grant , Pisano Michael , Zhan Fenghuang , Kim Sung Hoon , Katzenellenbogen John A. , Katzenellenbogen Benita S. , Hari Parameswaran , Janz Siegfried
Published: July 2022

The transcription factor, forkhead box M1 (FOXM1), has been implicated in the natural history and outcome of newly diagnosed high-risk myeloma (HRMM) and relapsed/refractory myeloma (RRMM), but the mechanism with which FOXM1 promotes the growth of neoplastic plasma cells is poorly understood. Here we show that FOXM1 is a positive regulator of myeloma metabolism that greatly impacts the bioenergetic pathways of glycolysis and oxidative phosphorylation (OxPhos). Using FOXM1-deficient myeloma cells as principal experimental model system, we find that FOXM1 increases glucose uptake, lactate output, and oxygen consumption in myeloma. We demonstrate that the novel 1,1-diarylethylene small-compound FOXM1 inhibitor, NB73, suppresses myeloma in cell culture and human-in-mouse xenografts using a mechanism that includes enhanced proteasomal FOXM1 degradation. Consistent with the FOXM1-stabilizing chaperone function of heat shock protein 90 (HSP90), the HSP90 inhibitor, geldanamycin, collaborates with NB73 in slowing down myeloma. These findings define FOXM1 as a key driver of myeloma metabolism and underscore the feasibility of targeting FOXM1 for new approaches to myeloma therapy and prevention.
TGFβR-1/ALK5 inhibitor RepSox induces enteric glia-to-neuron transition and influences gastrointestinal mobility in adult mice
Authors: Shi Chang-jie , Lian Jun-jiang , Zhang Bo-wen , Cha Jia-xue , Hua Qiu-hong , Pi Xiao-ping , Hou Yu-jun , Xie Xin , Zhang Ru
Published: July 2022

Promoting adult neurogenesis in the enteric nervous system (ENS) may be a potential therapeutic approach to cure enteric neuropathies. Enteric glial cells (EGCs) are the most abundant glial cells in the ENS. Accumulating evidence suggests that EGCs can be a complementary source to supply new neurons during adult neurogenesis in the ENS. In the brain, astrocytes have been intensively studied for their neuronal conversion properties, and small molecules have been successfully used to induce the astrocyte-to-neuron transition. However, research on glia-to-neuron conversion in the ENS is still lacking. In this study, we used GFAP-Cre:Rosa-tdTomato mice to trace glia-to-neuron transdifferentiation in the ENS in vivo and in vitro. We showed that GFAP promoter-driven tdTomato exclusively labelled EGCs and was a suitable marker to trace EGCs and their progeny cells in the ENS of adult mice. Interestingly, we discovered that RepSox or other ALK5 inhibitors alone induced efficient transdifferentiation of EGCs into neurons in vitro. Knockdown of ALK5 further confirmed that the TGFβR-1/ALK5 signalling pathway played an essential role in the transition of EGCs to neurons. RepSox-induced neurons were Calbindin- and nNOS-positive and displayed typical neuronal electrophysiological properties. Finally, we showed that administration of RepSox (3, 10 mg· kg−1 ·d−1, i.g.) for 2 weeks significantly promoted the conversion of EGCs to neurons in the ENS and influenced gastrointestinal motility in adult mice. This study provides a method for efficiently converting adult mouse EGCs into neurons by small-molecule compounds, which might be a promising therapeutic strategy for gastrointestinal neuropathy.
Periarteriolar spaces modulate cerebrospinal fluid transport into brain and demonstrate altered morphology in aging and Alzheimer’s disease
Authors: Mestre Humberto , Verma Natasha , Greene Thom D. , Lin LiJing A. , Ladron-de-Guevara Antonio , Sweeney Amanda M. , Liu Guojun , Thomas V. Kaye , Galloway Chad A. , de Mesy Bentley Karen L. , Nedergaard Maiken , Mehta Rupal I.
Published: July 2022

Perivascular spaces (PVS) drain brain waste metabolites, but their specific flow paths are debated. Meningeal pia mater reportedly forms the outermost boundary that confines flow around blood vessels. Yet, we show that pia is perforated and permissive to PVS fluid flow. Furthermore, we demonstrate that pia is comprised of vascular and cerebral layers that coalesce in variable patterns along leptomeningeal arteries, often merging around penetrating arterioles. Heterogeneous pial architectures form variable sieve-like structures that differentially influence cerebrospinal fluid (CSF) transport along PVS. The degree of pial coverage correlates with macrophage density and phagocytosis of CSF tracer. In vivo imaging confirms transpial influx of CSF tracer, suggesting a role of pia in CSF filtration, but not flow restriction. Additionally, pial layers atrophy with age. Old mice also exhibit areas of pial denudation that are not observed in young animals, but pia is unexpectedly hypertrophied in a mouse model of Alzheimer’s disease. Moreover, pial thickness correlates with improved CSF flow and reduced β-amyloid deposits in PVS of old mice. We show that PVS morphology in mice is variable and that the structure and function of pia suggests a previously unrecognized role in regulating CSF transport and amyloid clearance in aging and disease.
Ligand-induced transmembrane conformational coupling in monomeric EGFR
Authors: Srinivasan Shwetha , Regmi Raju , Lin Xingcheng , Dreyer Courtney A. , Chen Xuyan , Quinn Steven D. , He Wei , Coleman Matthew A. , Carraway Kermit L. , Zhang Bin , Schlau-Cohen Gabriela S.
Published: July 2022

Single pass cell surface receptors regulate cellular processes by transmitting ligand-encoded signals across the plasma membrane via changes to their extracellular and intracellular conformations. This transmembrane signaling is generally initiated by ligand binding to the receptors in their monomeric form. While subsequent receptor-receptor interactions are established as key aspects of transmembrane signaling, the contribution of monomeric receptors has been challenging to isolate due to the complexity and ligand-dependence of these interactions. By combining membrane nanodiscs produced with cell-free expression, single-molecule Förster Resonance Energy Transfer measurements, and molecular dynamics simulations, we report that ligand binding induces intracellular conformational changes within monomeric, full-length epidermal growth factor receptor (EGFR). Our observations establish the existence of extracellular/intracellular conformational coupling within a single receptor molecule. We implicate a series of electrostatic interactions in the conformational coupling and find the coupling is inhibited by targeted therapeutics and mutations that also inhibit phosphorylation in cells. Collectively, these results introduce a facile mechanism to link the extracellular and intracellular regions through the single transmembrane helix of monomeric EGFR, and raise the possibility that intramolecular transmembrane conformational changes upon ligand binding are common to single-pass membrane proteins.
Discovery of archaeal fusexins homologous to eukaryotic HAP2/GCS1 gamete fusion proteins
Authors: Moi David , Nishio Shunsuke , Li Xiaohui , Valansi Clari , Langleib Mauricio , Brukman Nicolas G. , Flyak Kateryna , Dessimoz Christophe , de Sanctis Daniele , Tunyasuvunakool Kathryn , Jumper John , Graña Martin , Romero Héctor , Aguilar Pablo S. , Jovine Luca , Podbilewicz Benjamin
Published: July 2022

Sexual reproduction consists of genome reduction by meiosis and subsequent gamete fusion. The presence of genes homologous to eukaryotic meiotic genes in archaea and bacteria suggests that DNA repair mechanisms evolved towards meiotic recombination. However, fusogenic proteins resembling those found in gamete fusion in eukaryotes have so far not been found in prokaryotes. Here, we identify archaeal proteins that are homologs of fusexins, a superfamily of fusogens that mediate eukaryotic gamete and somatic cell fusion, as well as virus entry. The crystal structure of a trimeric archaeal fusexin (Fusexin1 or Fsx1) reveals an archetypical fusexin architecture with unique features such as a six-helix bundle and an additional globular domain. Ectopically expressed Fusexin1 can fuse mammalian cells, and this process involves the additional globular domain and a conserved fusion loop. Furthermore, archaeal fusexin genes are found within integrated mobile elements, suggesting potential roles in cell-cell fusion and gene exchange in archaea, as well as different scenarios for the evolutionary history of fusexins.
Decatungstate-catalyzed radical disulfuration through direct C-H functionalization for the preparation of unsymmetrical disulfides
Authors: Zhang Jingjing , Studer Armido
Published: July 2022

Unsymmetrical disulfides are widely found in the areas of food chemistry, pharmaceutical industry, chemical biology and polymer science. Due the importance of such disulfides in various fields, general methods for the nondirected intermolecular disulfuration of C-H bonds are highly desirable. In this work, the conversion of aliphatic C(sp3)-H bonds and aldehydic C(sp2)-H bonds into the corresponding C-SS bonds with tetrasulfides (RSSSSR) as radical disulfuration reagents is reported. The decatungstate anion ([W10O32]4−) as photocatalyst is used for C-radical generation via intermolecular hydrogen atom transfer in combination with cheap sodium persulfate (Na2S2O8) as oxidant. Herein a series of valuable acyl alkyl disulfides, important precursors for the generation of RSS-anions, and unsymmetrical dialkyl disulfides are synthesized using this direct approach. To demonstrate the potential of the method for late-stage functionalization, approved drugs and natural products were successfully C-H functionalized.
Ultra-wide bandgap semiconductor Ga2O3 power diodes
Authors: Zhang Jincheng , Dong Pengfei , Dang Kui , Zhang Yanni , Yan Qinglong , Xiang Hu , Su Jie , Liu Zhihong , Si Mengwei , Gao Jiacheng , Kong Moufu , Zhou Hong , Hao Yue
Published: July 2022

Ultra-wide bandgap semiconductor Ga2O3based electronic devices are expected to perform beyond wide bandgap counterparts GaN and SiC. However, the reported power figure-of-merit hardly can exceed, which is far below the projected Ga2O3material limit. Major obstacles are high breakdown voltage requires low doping material and PN junction termination, contradicting with low specific on-resistance and simultaneous achieving of n- and p-type doping, respectively. In this work, we demonstrate that Ga2O3heterojunction PN diodes can overcome above challenges. By implementing the holes injection in the Ga2O3, bipolar transport can induce conductivity modulation and low resistance in a low doping Ga2O3material. Therefore, breakdown voltage of 8.32 kV, specific on-resistance of 5.24 mΩ⋅cm2, power figure-of-merit of 13.2 GW/cm2, and turn-on voltage of 1.8 V are achieved. The power figure-of-merit value surpasses the 1-D unipolar limit of GaN and SiC. Those Ga2O3power diodes demonstrate their great potential for next-generation power electronics applications.
Determining the interlayer shearing in twisted bilayer MoS2 by nanoindentation
Authors: Sun Yufei , Wang Yujia , Wang Enze , Wang Bolun , Zhao Hengyi , Zeng Yongpan , Zhang Qinghua , Wu Yonghuang , Gu Lin , Li Xiaoyan , Liu Kai
Published: July 2022

The rise of twistronics has increased the attention of the community to the twist-angle-dependent properties of two-dimensional van der Waals integrated architectures. Clarification of the relationship between twist angles and interlayer mechanical interactions is important in benefiting the design of two-dimensional twisted structures. However, current mechanical methods have critical limitations in quantitatively probing the twist-angle dependence of two-dimensional interlayer interactions in monolayer limits. Here we report a nanoindentation-based technique and a shearing-boundary model to determine the interlayer mechanical interactions of twisted bilayer MoS2. Both in-plane elastic moduli and interlayer shear stress are found to be independent of the twist angle, which is attributed to the long-range interaction of intermolecular van der Waals forces that homogenously spread over the interfaces of MoS2. Our work provides a universal approach to determining the interlayer shear stress and deepens the understanding of twist-angle-dependent behaviours of two-dimensional layered materials.
Entangling single atoms over 33 km telecom fibre
Authors: van Leent Tim , Bock Matthias , Fertig Florian , Garthoff Robert , Eppelt Sebastian , Zhou Yiru , Malik Pooja , Seubert Matthias , Bauer Tobias , Rosenfeld Wenjamin , Zhang Wei , Becher Christoph , Weinfurter Harald
Published: July 2022

Quantum networks promise to provide the infrastructure for many disruptive applications, such as efficient long-distance quantum communication and distributed quantum computing1,2. Central to these networks is the ability to distribute entanglement between distant nodes using photonic channels. Initially developed for quantum teleportation3,4 and loophole-free tests of Bell’s inequality5,6, recently, entanglement distribution has also been achieved over telecom fibres and analysed retrospectively7,8. Yet, to fully use entanglement over long-distance quantum network links it is mandatory to know it is available at the nodes before the entangled state decays. Here we demonstrate heralded entanglement between two independently trapped single rubidium atoms generated over fibre links with a length up to 33 km. For this, we generate atom–photon entanglement in two nodes located in buildings 400 m line-of-sight apart and to overcome high-attenuation losses in the fibres convert the photons to telecom wavelength using polarization-preserving quantum frequency conversion9. The long fibres guide the photons to a Bell-state measurement setup in which a successful photonic projection measurement heralds the entanglement of the atoms10. Our results show the feasibility of entanglement distribution over telecom fibre links useful, for example, for device-independent quantum key distribution11–13 and quantum repeater protocols. The presented work represents an important step towards the realization of large-scale quantum network links.
Accounting for the aircraft emissions of China’s domestic routes during 2014–2019
Authors: Cui Qiang , Chen Bin , Lei Yi-lin
Published: July 2022

The “13th Five-Year Plan” of civil aviation energy conservation and emission reduction impacts China’s domestic aviation exchanges. However, few researchers pay attrition to the impact of the 13th Five-Year Plan on aviation emissions. This paper intends to calculate the emissions of six pollutants (CO2, CO, HC, NOx, PM2.5, and SO2) in China’s domestic airlines from 2014 to 2019 and explore the impact of the 13th Five-Year Plan on emissions changes. In this paper, the improved BFFM2-FOA-FPM method is used to unify the calculation of CO2 and other aviation emissions. The error rate between the estimate and the official data was about 6.45%. The results show that the 13th Five-Year Plan has impacted on aviation emissions, including the number of routes and airlines, aircraft configuration, air routes, and airline unit turnover emissions. Additionally, the 13th Five-Year Plan’s effect is not significant, and it does not promote the reduction of emissions on domestic routes.
Deep learning for fast low-field MRI acquisitions
Authors: Ayde Reina , Senft Tobias , Salameh Najat , Sarracanie Mathieu
Published: July 2022

Low-field (LF) MRI research currently gains momentum from its potential to offer reduced costs and reduced footprints translating into wider accessibility. However, the impeded signal-to-noise ratio inherent to lower magnetic fields can have a significant impact on acquisition times that challenges LF clinical relevance. Undersampling is an effective way to speed up acquisitions in MRI, and recent work has shown encouraging results when combined with deep learning (DL). Yet, training DL models generally requires large databases that are not yet available at LF regimes. Here, we demonstrate the capability of Residual U-net combined with data augmentation to reconstruct magnitude and phase information of undersampled LF MRI scans at 0.1 T with a limited training dataset (n = 10). The model performance was first evaluated in a retrospective study for different acceleration rates and sampling patterns. Ultimately, the DL approach was validated on prospectively acquired, fivefold undersampled LF data. With varying performances associated to the adopted sampling scheme, our results show that the approach investigated can preserve the global structure and the details sharpness in the reconstructed magnitude and phase images. Overall, promising results could be obtained on acquired LF MR images that may bring this research closer to clinical implementation.
Ironing out the wrinkles and folds in the epidemiology of skin fold dermatitis in dog breeds in the UK
Authors: O’NeillI Dan G. , Rowe Dara , Brodbelt Dave C. , Pegram Camilla , Hendricks Anke
Published: July 2022

Skin fold dermatitis (intertrigo) is an inflammatory process of closely apposing skin surfaces. Extreme conformations towards folded skin in many dog breeds are linked with higher risk. Using anonymised primary-care veterinary data from the VetCompass Programme, this study aimed to report the frequency, demographic risk factors and clinical management for skin fold dermatitis in the UK. Risk factor analysis used random effects multivariable logistic regression modelling. From a study population of 905,553 dogs, the one-year period prevalence in dogs overall was 0.37% (95% CI 0.35–0.39). Diagnosis was supported by laboratory testing in 4.21% cases. Systemic antibiosis was used in 42.30% cases. Compared with crossbreed dogs, the most highly predisposed breeds were English Bulldog (odds ratio [OR] 49.07, 95% CI 37.79–63.70), French Bulldog (OR 25.92, 95% CI 19.62–34.26,) and Pug (OR 16.27, 95% CI 12.20–21.69). The most protected breeds were Yorkshire Terrier (OR 0.14, 95% CI 0.03–0.56), Border Collie (OR 0.31, 95% CI 0.11–0.84), Jack Russell Terrier (OR 0.53, 95% CI 0.30–0.92) and Labrador Retriever (OR 0.57, 95% CI 0.35–0.93). This study adds further evidence to the welfare concerns around high popularity of dog breeds with extreme conformations. The three breeds with by far the highest odds of skin fold dermatitis represent an extreme brachycephalic conformation.
Acidic condition accelerates cation release from purple rock in Southwestern China
Authors: Zhao Jixia , Li Chunpei , Lu Chuanhao , Deng Limei , Liu Gangcai , Fan Maopan
Published: July 2022

In spite of the fact that rock weathering performs an essential task in the evolution of the Earth’s surface, the quantitative assessment between pH and rates of chemical weathering remain unclear. This study aims to characterize the chemical weathering rate of purple rocks and then develops a model to calculate the release rates of cations (K+, Na+, Ca2+and Mg2+) under various pH conditions. Two types of purple rock were sampled from the Shaximiao Group (J2s) and Penglaizhen Group (J3p), and a series of laboratory experiments were performed by soaking the purple rocks in solutions with pHs from 2.5 to 7.0, over 24 treatment cycles. The results showed that the release rates of cations apparently increased as the pH decreased. The release of Ca2+was the dominant process of chemical weathering in J3p under various pH treatments, while K+and Na+were remarkably high in J2s (with the exception of the pH 2.5 treatment). Quantitative analysis revealed that the rate of cation release was significantly related to the H+concentration (p < 0.001) and the air temperature (p < 0.001). The relationship between cation release and acidity was found to be an exponential function. Our results suggested that solution acidity serves as an important driving force for cation release rates from purple rocks and that environmental acidification would enhance rock weathering.
Assessing Asiatic cheetah’s individual diet using metabarcoding and its implication for conservation
Authors: Khalatbari Leili , Egeter Bastian , Abolghasemi Hamed , Hakimi Ehsan , Ghadirian Taher , Khaleghi Hamidi Amir Hosein , Jowkar Houman , Breitenmoser Urs , Brito José Carlos
Published: July 2022

Knowledge on diet composition allows defining well-targeted conservation measures of large carnivores. Little is known about ecology of critically endangered Asiatic cheetah, especially the overall diet and its possible regional differences. We used cheetah scats, metabarcoding technique and microsatellite markers to assess the individual and overall diet composition of the species across its entire range in Asia. Cheetahs were primarily predating on mouflon; following by ibex, cape hare and goitered gazelle. Despite their high availability, small-sized livestock was never detected. Goitered gazelles were only detected in an area where the habitat is mainly flatlands. In hilly areas, mouflon was the most frequent prey item taken. Ibex was typically taken in rugged terrain, but mouflon was still the most frequently consumed item in these habitats. High consumption of mouflon in comparison to goitered gazelle suggests that human pressure on lowland habitats has possibly forced Asiatic cheetahs to occupy suboptimal habitats where gazelles are less abundant. The protection of flatlands and the removal of livestock from them are needed to ensure the long-term survival of Asiatic cheetah. The laboratory and bioinformatics pipelines used in this study are replicable and can be used to address similar questions in other threatened carnivores.
Structural and biochemical analysis reveals how ferulic acid improves catalytic efficiency of Humicola grisea xylanase
Authors: Oliveira Izadora Cristina Moreira , Garay Aisel Valle , Souza Amanda Araújo , Valadares Napoleão Fonseca , Barbosa João Alexandre Ribeiro Gonçalves , Faria Fabrícia Paula , Freitas Sonia Maria
Published: July 2022

Humicolagriseavar.thermoideais an aerobic and thermophilic fungus that secretes the GH11 xylanase HXYN2 in the presence of sugarcane bagasse. In this study, HXYN2 was expressed inPichiapastorisand characterized biochemically and structurally in the presence of beechwood xylan substrate and ferulic acid (FA). HXYN2 is a thermally stable protein, as indicated by circular dichroism, with greater activity in the range of 40–50 °C and pH 5.0–9.0, with optimal temperature and pH of 50 °C and 6.0, respectively. FA resulted in a 75% increase in enzyme activity and a 2.5-fold increase in catalytic velocity, catalytic efficiency, and catalytic rate constant (kcat), with no alteration in enzyme affinity for the substrate. Fluorescence quenching indicated that FA forms a complex with HXYN2 interacting with solvent-exposed tryptophan residues. The binding constants ranged from moderate (pH 7.0 and 9.0) to strong (pH 4.0) affinity. Isothermal titration calorimetry, structural models and molecular docking suggested that hydrogen bonds and hydrophobic interactions occur in the aglycone region inducing conformational changes in the active site driven by initial and final enthalpy- and entropy processes, respectively. These results indicate a potential for biotechnological application for HXYN2, such as in the bioconversion of plant residues rich in ferulic acid.
Genome-wide identification and in silico analysis of NPF, NRT2, CLC and SLAC1/SLAH nitrate transporters in hexaploid wheat (Triticum aestivum)
Authors: Kumar Aman , Sandhu Nitika , Kumar Pankaj , Pruthi Gomsie , Singh Jasneet , Kaur Satinder , Chhuneja Parveen
Published: July 2022

Nitrogen transport is one of the most important processes in plants mediated by specialized transmembrane proteins. Plants have two main systems for nitrogen uptake from soil and its transport within the system—a low-affinity transport system and a high-affinity transport system. Nitrate transporters are of special interest in cereal crops because large amount of money is spent on N fertilizers every year to enhance the crop productivity. Till date four gene families of nitrate transporter proteins; NPF (nitrate transporter 1/peptide transporter family), NRT2 (nitrate transporter 2 family), the CLC (chloride channel family), and the SLAC/SLAH (slow anion channel-associated homologues) have been reported in plants. In our study, in silico mining of nitrate transporter genes along with their detailed structure, phylogenetic and expression analysis was carried out. A total of 412 nitrate transporter genes were identified in hexaploid wheat genome using HMMER based homology searches in IWGSC Refseq v2.0. Out of those twenty genes were root specific, 11 leaf/shoot specific and 17 genes were grain/spike specific. The identification of nitrate transporter genes in the close proximity to the previously identified 67 marker-traits associations associated with the nitrogen use efficiency related traits in nested synthetic hexaploid wheat introgression library indicated the robustness of the reported transporter genes. The detailed crosstalk between the genome and proteome and the validation of identified putative candidate genes through expression and gene editing studies may lay down the foundation to improve nitrogen use efficiency of cereal crops.