bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Vitamin D3 Supplementation vs. Dietary–Hygienic Measures on SARS-CoV-2 Infection Rates in Hospital Workers with 25-Hydroxyvitamin D3 [25(OH)D3] Levels ≥20 ng/mL
Authors: Romero-Ibarguengoitia Maria Elena , Gutiérrez-González Dalia , Cantú-López Carlos , Sanz-Sánchez Miguel Ángel , González-Cantú Arnulfo
Published: January 2023

Background: There is scant information on the effect of supplementation with vitamin D3 in SARS-CoV-2 infection cases when patient 25-hydroxyvitamin D3 [25(OH)D3] levels are between 20–100 ng/mL. We aimed to evaluate the effect of supplementation with vitamin D3 vs. dietary–hygienic measures on the SARS-CoV-2 infection rate in participants with serum 25(OH)D3 levels ≥20 ng/mL. Methods: This study was quasi-experimental. We invited hospital workers with 25(OH)D3 levels between 20–100 ng/mL and no previous SARS-CoV-2 infection. They were randomized as follows: treatment options were a) vitamin D3 supplementation (52,000 IU monthly, G1) or b) dietary–hygienic measures (G2). We conducted a 3- to 6-month follow-up of SARS-CoV-2 infections. Participants with 25(OH)D3 levels <20 ng/mL were also analyzed. We divided these latter participants depending on whether they were supplemented (G3) or not (G4). Results: We analyzed 198 participants, with an average age of 44.4 (SD 9) years, and 130 (65.7%) were women. G1 had fewer cases of SARS-CoV-2 infection than G2 after a follow-up of 3- to 6-months (p < 0.05). There were no differences between G3 and G4 at the 3- and 6-month follow-up cutoff points (p > 0.05). Using a mixed effect Cox regression analysis in the 164 participants that completed six months of follow-up, vitamin D3 supplementation appeared to act as a protective factor against SARS-CoV-2 infection (HR 0.21, p = 0.008) in G1 and G2. None of the participants treated with the supplementation doses had serum 25(OH)D3 levels >100 ng/mL. Conclusions: Vitamin D3 supplementation in participants with 25(OH)D3 levels between 20–100 ng/mL have a lower rate of SARS-CoV-2 infection compared with the use of dietary–hygienic measures at six months follow-up.
Fatal Puumala Hantavirus Infection in a Patient with Common Variable Immunodeficiency (CVID)
Authors: Steininger Philipp , Herbst Larissa , Bihlmaier Karl , Willam Carsten , Körper Sixten , Schrezenmeier Hubert , Klüter Harald , Pfister Frederick , Amann Kerstin , Weiss Sabrina , Krüger Detlev H. , Zimmermann Robert , Korn Klaus , Hofmann Jörg , Harrer Thomas
Published: January 2023

Puumala hantavirus (PUUV) infections usually show a mild or moderate clinical course, but may sometimes also lead to life-threatening disease. Here, we report on a 60-year-old female patient with common variable immunodeficiency (CVID) who developed a fatal PUUV infection with persistent renal failure, thrombocytopenia, and CNS infection with impaired consciousness and tetraparesis. Hantavirus-specific antibodies could not be detected due to the humoral immunodeficiency. Diagnosis and virological monitoring were based on the quantitative detection of PUUV RNA in blood, cerebrospinal fluid, bronchial lavage, and urine, where viral RNA was found over an unusually extended period of one month. Due to clinical deterioration and virus persistence, treatment with ribavirin was initiated. Additionally, fresh frozen plasma (FFP) from convalescent donors with a history of PUUV infection was administered. Despite viral clearance, the clinical condition of the patient did not improve and the patient died on day 81 of hospitalization. This case underlines the importance of the humoral immune response for the course of PUUV disease and illustrates the need for PCR-based virus diagnostics in those patients. Due to its potential antiviral activity, convalescent plasma should be considered in the therapy of severe hantavirus diseases.
Promoting the Circular Economy on an Island: Anaerobic Co-Digestion of Local Organic Substrates as a Possible Renewable Energy Source
Authors: Fernández-Rodríguez Juana , Di Berardino Mónica , Di Berardino Santino
Published: January 2023

The local waste co-digestion is an interesting option to tackle in reduced and isolated areas like the islands. The islands have limited territory and scarce fuel production. Moreover, organic waste can create serious environmental problems in soil, water and air. Anaerobic co-digestion (AcoD) is a technology fulfilling the concept of waste-to-energy (WtE) based on local resources. The valorisation of organic waste through AcoD on an island would prevent environmental impacts, while being a source of renewable energy. In this study, cow manure (outdoor and indoor), pig slurry, bird manure, kitchen waste, sewage sludge and oily lacteous waste produced on Island Terceira (Portugal) were tested in mesophilic −35 °C- Biochemical Methane Potential (BMP) co-digestion assays. The goals were to analyse the recalcitrant and high potential produced waste and to estimate the energetic supply source on the island. The cow manure and pig slurry were used as inocula and specific methanogenic activities (SMAs) were carried out. The results showed that both substrates have a significant methanogenic activity–SMA 0.11 g-COD/(g-VSS.d) and 0.085 g-COD/(g-VSS.d), respectively. All the studied combinations were feasible in AcoD, showing TS removals in the range of 19–37%; COD removals in the range 67–78% and specific methane yields from 0.14 to 0.22 L/gCOD removed, but some differences were found. The modified Gompertz model fitted the AcoD assays (R2 0.982–0.998). The maximum biogas production rate, Rmax. was highest in the AcoD of Cow+Pig+Oily and in the Cow+Pig+Sludge with 0.017 and 0.014 L/g-VSadded.day, respectively, and the lowest in Cow+Pig+Bird with 0.010 L/g-VSadded. In our AcoD studies, the bird manure limited the performance of the process, since it was recalcitrant to anaerobic degradation. On the other hand, the oily lacteous waste showed a great potential in the anaerobic digestion. The estimated biogas production, from the best-studied condition, could cover the 11.4% of the energy supply of the inhabitants. These preliminary results would prevent the environmental impact of organic waste on the island and promote the use of local waste in a circular economy scenario.
Genome Analysis Identifies a Novel Type III Secretion System (T3SS) Category in Vibrio Species
Authors: Zakaria Douaa , Matsuda Shigeaki , Iida Tetsuya , Hayashi Tetsuya , Arita Masanori
Published: January 2023

The nanomachine referred to as the type III secretion system (T3SS) is used by many Gram-negative pathogens or symbionts to inject their effector proteins into host cells to promote their infections or symbioses. Among the genera possessing T3SS is Vibrio, which consists of diverse species of Gammaproteobacteria including human pathogenic species and inhabits aquatic environments. We describe the genetic overview of the T3SS gene clusters in Vibrio through a phylogenetic analysis from 48 bacterial strains and a gene order analysis of the two previously known categories in Vibrio (T3SS1 and T3SS2). Through this analysis we identified a new T3SS category (named T3SS3) that shares similar core and related proteins (effectors, translocons, and chaperones) with the Ssa-Esc family of T3SSs in Salmonella, Shewanella, and Sodalis. The high similarity between T3SS3 and the Ssa-Esc family suggests a possibility of genetic exchange among marine bacteria with similar habitats.
The Influence of Symbiosis on the Proteome of the Exaiptasia Endosymbiont Breviolum minutum
Authors: Mashini Amirhossein Gheitanchi , Oakley Clinton A. , Beepat Sandeep S. , Peng Lifeng , Grossman Arthur R. , Weis Virginia M. , Davy Simon K.
Published: January 2023

The cellular mechanisms responsible for the regulation of nutrient exchange, immune response, and symbiont population growth in the cnidarian–dinoflagellate symbiosis are poorly resolved. Here, we employed liquid chromatography–mass spectrometry to elucidate proteomic changes associated with symbiosis in Breviolum minutum, a native symbiont of the sea anemone Exaiptasia diaphana (‘Aiptasia’). We manipulated nutrients available to the algae in culture and to the holobiont in hospite (i.e., in symbiosis) and then monitored the impacts of our treatments on host–endosymbiont interactions. Both the symbiotic and nutritional states had significant impacts on the B. minutum proteome. B. minutum in hospite showed an increased abundance of proteins involved in phosphoinositol metabolism (e.g., glycerophosphoinositol permease 1 and phosphatidylinositol phosphatase) relative to the free-living alga, potentially reflecting inter-partner signalling that promotes the stability of the symbiosis. Proteins potentially involved in concentrating and fixing inorganic carbon (e.g., carbonic anhydrase, V-type ATPase) and in the assimilation of nitrogen (e.g., glutamine synthase) were more abundant in free-living B. minutum than in hospite, possibly due to host-facilitated access to inorganic carbon and nitrogen limitation by the host when in hospite. Photosystem proteins increased in abundance at high nutrient levels irrespective of the symbiotic state, as did proteins involved in antioxidant defences (e.g., superoxide dismutase, glutathione s-transferase). Proteins involved in iron metabolism were also affected by the nutritional state, with an increased iron demand and uptake under low nutrient treatments. These results detail the changes in symbiont physiology in response to the host microenvironment and nutrient availability and indicate potential symbiont-driven mechanisms that regulate the cnidarian–dinoflagellate symbiosis.
The Cell Wall Deacetylases Spy1094 and Spy1370 Contribute to Streptococcus pyogenes Virulence
Authors: Aspell Tiger , Khemlani Adrina Hema J. , Tsai Catherine Jia-Yun , Loh Jacelyn Mei San , Proft Thomas
Published: January 2023

Streptococcus pyogenes, or Group A Streptococcus (GAS), is a strictly human pathogen that causes a wide range of diseases, including skin and soft tissue infections, toxic shock syndrome and acute rheumatic fever. We have recently reported that Spy1094 and Spy1370 of S. pyogenes serotype M1 are N-acetylglucosamine (GlcNAc) deacetylases. We have generated spy1094 and spy1370 gene deletion mutants in S. pyogenes and gain-of-function mutants in Lactococcus lactis. Similar to other cell wall deacetylases, our results show that Spy1094 and Spy1370 confer lysozyme-resistance. Furthermore, deletion of the genes decreased S. pyogenes virulence in a human whole blood killing assay and a Galleria mellonella (Greater wax moth) larvae infection model. Expression of the two genes in L. lactis resulted in increased lysozyme resistance and survival in whole human blood, and reduced survival of infected G. mellonella larvae. Deletion of the spy1370, but not the spy1094 gene, decreased resistance to the cationic antimicrobial peptide cecropin B, whereas both enzymes increased biofilm formation, probably resulting from the increase in positive charges due to deacetylation of the cell wall. In conclusion, Spy1094 and Spy1370 are important S. pyogenes virulence factors and might represent attractive targets for the development of antibacterial agents.
Giardia duodenalis Styles, 1902 Prevalence in Cattle (Bos taurus Linnaeus, 1758) in Europe: A Systematic Review
Authors: Mateusa Maira , Ozoliņa Zanda , Terentjeva Margarita , Deksne Gunita
Published: January 2023

Giardia duodenalis Styles, 1902 is an infectious agent which can cause enteritic disease in cattle (Bos taurus Linnaeus, 1758) worldwide. As a zoonotic protozoan, it is important to acknowledge Giardia prevalence and assemblages found in cattle and risk factors associated with the Giardia infection in herds. This systematic review aims to estimate the prevalence of G. duodenalis and its assemblages in cattle and to identify the risk factors associated with Giardia infection in cattle in Europe. A systematic review was performed according to Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines to collect information from published studies in Europe. A total of 1414 studies were identified and 17 relevant studies were included in this review. Mean Giardia prevalence in cattle in Europe was 35.1%, with the highest prevalence found in neonatal animals (39.6%), but mean heard prevalence was 67.0%. Mixed infections of Giardia assemblages A and E were found most frequently (55.6%), while assemblages A and B were found more frequently in animals under 24 months old. Risk factors, such as deep litter with run-out, prolonged calf contact with the dam, and seasonality, such as winter and spring, were found to be potential risk factors for Giardia presence in the herds.
An Overview on Exploitation of Graphene-Based Membranes: From Water Treatment to Medical Industry, Including Recent Fighting against COVID-19
Authors: Lavorato Cristina , Fontananova Enrica
Published: January 2023

Graphene and its derivatives have lately been the subject of increased attention for different environmental applications of membrane technology such as water treatment and air filtration, exploiting their antimicrobial and antiviral activity. They are interesting candidates as membrane materials for their outstanding mechanical and chemical stability and for their thin two-dimensional (2D) nanostructure with potential pore engineering for advanced separation. All these applications have evolved and diversified from discovery to today, and now graphene and graphene derivatives also offer fascinating opportunities for the fight against infective diseases such as COVID-19 thanks to their antimicrobial and antiviral properties. This paper presents an overview of graphene-based 2D materials, their preparation and use as membrane material for applications in water treatment and in respiratory protection devices.
Transporter-Mediated Drug Delivery
Authors: Gyimesi Gergely , Hediger Matthias A.
Published: January 2023

Transmembrane transport of small organic and inorganic molecules is one of the cornerstones of cellular metabolism. Among transmembrane transporters, solute carrier (SLC) proteins form the largest, albeit very diverse, superfamily with over 400 members. It was recognized early on that xenobiotics can directly interact with SLCs and that this interaction can fundamentally determine their efficacy, including bioavailability and intertissue distribution. Apart from the well-established prodrug strategy, the chemical ligation of transporter substrates to nanoparticles of various chemical compositions has recently been used as a means to enhance their targeting and absorption. In this review, we summarize efforts in drug design exploiting interactions with specific SLC transporters to optimize their therapeutic effects. Furthermore, we describe current and future challenges as well as new directions for the advanced development of therapeutics that target SLC transporters.
Recovery of Bioactive Compounds from Marine Organisms: Focus on the Future Perspectives for Pharmacological, Biomedical and Regenerative Medicine Applications of Marine Collagen
Authors: Rigogliuso Salvatrice , Campora Simona , Notarbartolo Monica , Ghersi Giulio
Published: January 2023

Marine environments cover more than 70% of the Earth’s surface and are among the richest and most complex ecosystems. In terms of biodiversity, the ocean represents an important source, still not widely exploited, of bioactive products derived from species of bacteria, plants, and animals. However, global warming, in combination with multiple anthropogenic practices, represents a serious environmental problem that has led to an increase in gelatinous zooplankton, a phenomenon referred to as jellyfish bloom. In recent years, the idea of “sustainable development” has emerged as one of the essential elements of green-economy initiatives; therefore, the marine environment has been re-evaluated and considered an important biological resource. Several bioactive compounds of marine origin are being studied, and among these, marine collagen represents one of the most attractive bio-resources, given its use in various disciplines, such as clinical applications, cosmetics, the food sector, and many other industrial applications. This review aims to provide a current overview of marine collagen applications in the pharmacological and biomedical fields, regenerative medicine, and cell therapy.
Chemical Composition, Antioxidant, and Antibiofilm Properties of Essential Oil from Thymus capitatus Plants Organically Cultured on the Greek Island of Lemnos
Authors: Maniki Eirini , Kostoglou Dimitra , Paterakis Nikolaos , Nikolaou Anastasios , Kourkoutas Yiannis , Papachristoforou Alexandros , Giaouris Efstathios
Published: January 2023

Essential oils (EOs) are mixtures of volatile plant secondary metabolites and have been exploited by humans for thousands of years for various purposes because of their many bioactivities. In this study, the EO from Thymus capitatus, a thyme species organically cultured on the Greek Island of Lemnos, was analyzed for its chemical composition (through GC-FID and GC-MS), antioxidant activity (AA), and total phenolic content (TPC), as well as its antimicrobial and antibiofilm actions against three important foodborne bacterial pathogens (Salmonella enterica ser. Typhimurium, Listeria monocytogenes, and Yersinia enterocolitica). For the latter investigations, the minimum inhibitory concentrations (MICs) and minimum biofilm inhibitory concentrations (MBICs) of the EO against the planktonic and biofilm growth of each pathogen were determined, together with the minimum biofilm eradication concentrations (MBECs). Results revealed that T. capitatus EO was rich in thymol, p-cymene, and carvacrol, presenting high AA and TPC (144.66 μmol TroloxTM equivalents and 231.32 mg gallic acid equivalents per g of EO, respectively), while its MICs and MBICs ranged from 0.03% to 0.06% v/v and 0.03% to 0.13% v/v, respectively, depending on the target pathogen. The EO was able to fully destroy preformed (mature) biofilms of all three pathogenic species upon application for 15 min, with MBECs ranging from 2.00 to 6.25% v/v. Overall, the results demonstrate that the EO of organically cultured T. capitatus presents strong antioxidant, antibacterial, and antibiofilm properties and could, therefore, be further exploited as a functional and antimicrobial natural formulation for food and health applications.
Ferritin-Coated SPIONs as New Cancer Cell Targeted Magnetic Nanocarrier
Authors: Affatigato Luisa , Licciardi Mariano , Bonamore Alessandra , Martorana Annalisa , Incocciati Alessio , Boffi Alberto , Militello Valeria
Published: January 2023

Superparamagnetic iron oxide nanoparticles (SPIONs) may act as an excellent theragnostic tool if properly coated and stabilized in a biological environment, even more, if they have targeting properties towards a specific cellular target. Humanized Archaeoglobus fulgidus Ferritin (HumAfFt) is an engineered ferritin characterized by the peculiar salt-triggered assembly-disassembly of the hyperthermophile Archaeoglobus fulgidus ferritin and is successfully endowed with the human H homopolymer recognition sequence by the transferrin receptor (TfR1 or CD71), overexpressed in many cancer cells in response to the increased demand of iron. For this reason, HumAfFt was successfully used in this study as a coating material for 10 nm SPIONs, in order to produce a new magnetic nanocarrier able to discriminate cancer cells from normal cells and maintain the potential theragnostic properties of SPIONs. HumAfFt-SPIONs were exhaustively characterized in terms of size, morphology, composition, and cytotoxicity. The preferential uptake capacity of cancer cells toward HumAfFt-SPIONs was demonstrated in vitro on human breast adenocarcinoma (MCF7) versus normal human dermal fibroblast (NHDF) cell lines.
Heavily Gd-Doped Non-Toxic Cerium Oxide Nanoparticles for MRI Labelling of Stem Cells
Authors: Popov Anton L. , Savintseva Irina V. , Kozlova Taisiya O. , Ivanova Olga S. , Zhukov Ivan V. , Baranchikov Alexander E. , Yurkovskaya Alexandra V. , Savelov Andrey A. , Ermakov Artem M. , Popova Nelli R. , Ivanov Konstantin L. , Ivanov Vladimir K.
Published: January 2023

Recently, human mesenchymal stem cells (hMSc) have attracted a great deal of attention as potential therapeutic agents in the treatment of socially significant diseases. Despite substantial advances in stem-cell therapy, the biological mechanisms of hMSc action after transplantation remain unclear. The use of magnetic resonance imaging (MRI) as a non-invasive method for tracking stem cells in the body is very important for analysing their distribution in tissues and organs, as well as for ensuring control of their lifetime after injection. Herein, detailed experimental data are reported on the biocompatibility towards hMSc of heavily gadolinium-doped cerium oxide nanoparticles (Ce0.8Gd0.2O2−x) synthesised using two synthetic protocols. The relaxivity of the nanoparticles was measured in a magnetic field range from 1 mT to 16.4 T. The relaxivity values (r1 = 11 ± 1.2 mM−1 s−1 and r1 = 7 ± 1.2 mM−1 s−1 in magnetic fields typical of 1.5 and 3 T MRI scanners, respectively) are considerably higher than those of the commercial Omniscan MRI contrast agent. The low toxicity of gadolinium-doped ceria nanoparticles to hMSc enables their use as an effective theranostic tool with improved MRI-contrasting properties.
Thermal-Assisted Laser Fabrication of Broadband Ultralow Reflectance Surface by Combining Marangoni Flow with In Situ Deposition
Authors: Yin Jingbo , Yan Huangping , Zhou Rui , Li Yuanzhe , He Anna
Published: January 2023

Functional surfaces with broadband ultralow optical reflectance have many potential applications in the fields of enhancing solar energy utilization, stray light shielding, infrared stealth, and so on. To fabricate broadband anti-reflection surfaces with low cost, high quality, and more controllability, a strategy of preparing multi-scale structures by thermal-assisted nanosecond laser was proposed. This strategy combines laser ablation with Marangoni flow of molten materials and in situ deposition of nanoparticles. The thermal-assisted strategy increases the depth to width ratio of the anti-reflection structures. The average reflectance of laser-textured TC4 (Ti-6Al-4V) surface is as low as 1.71% in the wavelength range of 200–2250 nm and 7.8% in the 2500–25,000 nm. The ultra-low reflectance surface has a significantly enhanced photothermal conversion performance. Meanwhile, the anti-reflection effect can be extended to the mid-infrared band, which has potential stealth application prospect. This synergetic manufacturing strategy has wide adaptability of materials, which provides new paths for the preparation of broadband ultralow reflectance surface. Moreover, this thermal-assisted laser fabrication strategy is prospective in the preparation of other functional micro-nano structures.
Investigating the Relationship between Fear of Failure and the Delivery of End-of-Life Care: A Questionnaire Study
Authors: Latham John S. , Sawyer Hannah , Butchard Sarah , Mason Stephen R. , Sartain Kathryn
Published: January 2023

Objective: To investigate whether fear of failure (FOF) influences a clinician’s perception of how confident and comfortable they are in their delivery of end-of-life (EOL) care. Methods: Cross-sectional questionnaire study with recruitment of physicians and nurses across two large NHS hospital trusts in the UK and national UK professional networks. A total of 104 physicians and 101 specialist nurses across 20 hospital specialities provided data that were analysed using a two-step hierarchical regression. Results: The study validated the PFAI measure for use in medical contexts. Number of EOL conversations, gender, and role were shown to impact confidence and comfortableness with EOL care. Four FOF subscales did show a significant relationship with perceived delivery of EOL care. Conclusion: Aspects of FOF can be shown to negatively impact the clinician experience of delivering EOL care. Clinical Implications: Further study should explore how FOF develops, populations that are more susceptible, sustaining factors, and its impact on clinical care. Techniques developed to manage FOF in other populations can now be investigated in a medical population.
Characterizing Pharmacist Perspectives on Utilizing a Personalized Family Medication Safety Plan for Opioid Education with Adolescents and Parents
Authors: Abraham Olufunmilola , Peters Joanne , Peterson Kourtney A.
Published: January 2023

Background: Exposure to prescription opioids during adolescence is associated with an increased risk of future opioid misuse. The pervasive and growing impact of the opioid epidemic requires evidence-based, co-designed interventions targeted at adolescents. MedSMA℞T Families is an intervention tailored to educate adolescents and their families about opioid misuse prevention and consists of two parts: the MedSMA℞T: Adventures in PharmaCity videogame and the family medication safety plan (FMSP). Objective: This study sought to explore pharmacists’ perceptions of using the family medication safety plan to facilitate opioid education among parents and their adolescents. The purpose of this project was to also gather information for iterative adaptations to improve implementation and dissemination of the FMSP in pharmacy settings. Methods: Pharmacists were recruited from Pharmacy Practice Enhancement and Action Research Link (PearlRx) and the Pharmacy Society of Wisconsin (PSW). Twenty-one pharmacist interviews were conducted between September 2021 and March 2022. Consenting pharmacists reviewed the FMSP. Then, semi-structured interviews were conducted, recorded, and transcribed. Inductive thematic analyses were performed using NVivo software. Results: Four prevalent themes emerged: (1) the purpose of FMSP as a communication tool, (2) instructions to clarify how to use FMSP, (3) barriers to using FMSP, and (4) suggestions to improve FMSP format. Most pharmacists described the FMSP as a tool to encourage interactive opioid conversations between adolescents, families, and pharmacists. Pharmacists suggested creating multiple customizable formats and incorporating instructions on how to use the FMSP. Conclusions: Pharmacists noted that the FMSP was an interactive and engaging communication tool to tailor opioid consultations with adolescents and their families. Patients might use the FMSP as a visual cue to help think of what question(s) they should ask pharmacists. Pharmacists stated that the FMSP could facilitate tailored opioid safety communication and medication consultations. Insights will inform future medication misuse prevention interventions as well as adaptation.
Metabolic Syndrome in Adults Receiving Clozapine; The Need for Pharmacist Support
Authors: Hurley Kathleen , O’Brien Sinead , Halleran Ciaran , Byrne Derina , Foley Erin , Cunningham Jessica , Hoctor Fionnuala , Sahm Laura J.
Published: January 2023

People who are diagnosed with treatment resistant schizophrenia (TRS) are likely to have clozapine as a therapeutic management option. There is a high prevalence of metabolic syndrome in patients receiving clozapine. To mitigate against this, monitoring of weight, waist circumference, lipid profile, glycated haemoglobin (HbA1c), fasting blood glucose (FBG) and blood pressure (BP) is recommended. The aims of this study were to examine the prevalence of metabolic syndrome and whether any variables were correlated with its development, and to highlight any opportunities for the pharmacist to offer support. This study was conducted in an urban hospital and its associated Clozapine Clinic in Cork, Ireland. A retrospective audit assessed the prevalence of metabolic syndrome using the International Diabetes Federation (IDF) criteria. Patients were eligible for inclusion if they were aged 18 years or more, registered with the Clozapine Clinic, and had the capacity to provide informed consent. All data were entered into Microsoft® Excel ® (Microsoft Corporation) and further statistical analysis was undertaken using R, t-tests, Fisher’s Exact Test and Mann–Whitney U tests as appropriate, and p ≤ 0.05 was considered statistically significant. Of 145 patients (32% female; mean age (SD) 45.3 (±11.7) years; 86.2% living independently/in family home), nearly two thirds (n = 86, 59.3%) were diagnosed with metabolic syndrome. The mean age of participants with metabolic syndrome was 44.4 years (SD = 10.8), similar to the 46.6 years (SD = 12.8) for those without. Variables that were identified to be statistically significantly associated with metabolic syndrome included waist circumference, weight, triglycerides, high density lipoprotein-cholesterol (HDL-C), BP, FBG and HbA1c. The high incidence of metabolic syndrome in this patient population highlights the need for continued physical health monitoring of these patients to ameliorate the risk of developing metabolic syndrome.
Silicon Waveguide Sensors for Carbon Dioxide Gas Sensing in the Mid-Infrared Region
Authors: Song Yuxia , Li Baoshuai , Zhang Huiyuan , Li Mingyu , Li Qiushun , He Jian-Jun
Published: January 2023

Two optical waveguide sensors based on SOS (silicon-on-sapphire) for detecting CO2 are theoretically proposed. The operational wavelength is 4.23 μm, which is the maximum absorption line of CO2. The power confinement factor (η) value is over 40% and 50%, the propagation loss is 0.98 dB/cm and 2.99 dB/cm, respectively, in the slot waveguide and SWGS (subwavelength grating slot) waveguide. An inverted tapered structure is used for the transition from strip waveguide to slot waveguide and constitutes the sensing absorption region, with the coupling efficiency that can reach more than 90%. When the optimal absorption length of the slot waveguide and SWGS waveguide is 1.02 cm and 0.33 cm, respectively, the maximum sensitivity can reach 6.66 × 10−5 (ppm−1) and 2.60 × 10−5 (ppm−1). Furthermore, taking the slot waveguide as an example, spiral and meander structures enable the long-distance sensing path to integrate into a small area.
Variation in Shoot, Peduncle and Fruit Growth of Lagenaria siceraria Landraces
Authors: Buthelezi Lungelo Given , Mavengahama Sydney , Sibiya Julia , Ntuli Nontuthuko Rosemary
Published: January 2023

Lagenaria siceraria (Molina) Standley is a prominent food source as almost all its plant parts are edible. However, no studies have recorded the changes in shoots, peduncles and fruits during its growth. Hence, this study aimed to record changes in shoot traits and relate the peduncle to the fruit traits of L. siceraria landraces across different growth stages. Changes in shoots, peduncles and fruits during growth were compared within and among landraces using analysis of variance, correlation, principal component analysis, cluster analysis and heritability estimates. Almost all landraces had harvestable shoots at 42 days after sowing. Peduncles became shorter and wider as the fruits elongated. Shoots, peduncles and fruits correlated positively with each other. The informative principal components had a total variability of 84.488%, with a major contribution from shoot traits. The biplot and dendrogram clustered landraces with similar growth habits and the harvestable shoot and fruit attributes into three clusters, but KRI and NSRC formed singlets. Shoot width (60.2%) and peduncle length (55.2%) had high heritability estimates. The general low heritability estimates and genetic advances indicated the presence of non-additive gene action. This study is the first report on changes in harvested shoots and the relationship between peduncles and fruits during growth.
Taxonomy and Distribution of Spiraea hypericifolia in Italy and Typification of the Name S. flabellata (Rosaceae)
Authors: Conti Fabio , Bartolucci Fabrizio
Published: January 2023

Spiraea hypericifolia is a Eurasiatic species, distributed from SW Europe to C and SW Asia. In Italy, only the alien S. hypericifolia subsp. obovata was recorded, as naturalized in the Central Apennines. Spiraea flabellata was described from Abruzzo (Central Apennines, Italy) by Gussone in 1826 and is currently regarded as a synonym of S. hypericifolia subsp. obovata. Based on the study of living plants from locus classicus and the analysis of the original material traced in BOLO and NAP, S. flabellata should be referred instead to S. hypericifolia subsp. hypericifolia, a taxon reported here for the first time in Italy. The name S. flabellata is lectotypified with a specimen kept in NAP. Based on our study, S. hypericifolia subsp. obovata should be excluded from Italian flora. Spiraea hypericifolia subsp. hypericifolia should be considered native to Italy and added to the contingent of steppe plants of phytogeographic and conservation interest recorded in the Central Apennines. Finally, the conservation status assessment of S. hypericifolia subsp. hypericifolia according to IUCN categories and criteria, is proposed and discussed.
Plant-Based Solutions for Non-Productive Sites Useful in the Management of Dry Land
Authors: Hanus-Fajerska Ewa , Kępka Katarzyna , Kruszyna Cezary , Kamińska Iwona
Published: January 2023

The article presents an overview of research conducted in recent years, i.e., from 2004 until now. The study has been prompted by the threat of drought over large land areas which, as a result of current climate change, may lead to desertification in dry and hot regions of the world. For the same reason, large areas of farmland are affected by drought stress. At the same time, rising air temperatures result in a significant intensification of evaporation and a gradual increase in soil salinity. This applies in particular to acres of farmland, forested areas, and green areas of cities, as well as degraded land or brownfields. As the crop stability is threatened, the food base of the world’s population is at risk and, additionally, in areas of industrial districts, people’s health is in decline. Due to these multistress conditions for plant growth, we propose a review of the current literature which addresses the possibility of counteracting these unfavorable phenomena through the appropriate selection of plant species and, when only applicable, also through specific agroecological treatments. A selection of herbaceous and woody plants useful for cultivation on saline marginal lands was proposed.
Reuse of Carbon Fibers and a Mechanically Recycled CFRP as Rod-like Fillers for New Composites: Optimization and Process Development
Authors: Butenegro José Antonio , Bahrami Mohsen , Martínez Miguel Ángel , Abenojar Juana
Published: January 2023

The rising amount of carbon fiber reinforced polymer (CFRP) composite waste requires new processes for reintroducing waste into the production cycle. In the present research, the objective is the design and study of a reuse process for carbon fibers and CFRP by mechanical recycling consisting of length and width reduction, obtaining rods and reintegrating them as fillers into a polymeric matrix. Preliminary studies are carried out with continuous and discontinuous unidirectional fibers of various lengths. The processing conditions are then optimized, including the length of the reinforcement, the need for a plasma surface treatment and/or for resin post-curing. The resin is thermally characterized by differential scanning calorimetry (DSC), while the composites are mechanically characterized by tensile strength tests, completed by a factorial design. In addition, the composites tested are observed by scanning electron microscopy (SEM) to study the fracture mechanics. Optimal processing conditions have been found to reduce the reinforcement length to 40 mm while maintaining the mechanical properties of continuous reinforcement. Furthermore, the post-curing of the epoxy resin used as matrix is required, but a low-pressure plasma treatment (LPPT) is not recommended on the reinforcement.
Development of Experimental Techniques for the Phase Equilibrium Study in the Pb-Fe-O-S-Si System Involving Gas, Slag, Matte, Lead Metal and Tridymite Phases
Authors: Hidayat Taufiq , Fallah-Mehrjardi Ata , Shevchenko Maksym , Hayes Peter C. , Jak Evgueni
Published: January 2023

Present society challenges, including metal scarcity, recycling, and environmental restrictions, resulted in the increased complexity and variability of metallurgical feed streams. Metallurgical processes involving complex lead and copper-containing slag and matte phases are now commonly used in response. Optimization of existing and development of new metallurgical processes require fundamental information on slag–matte phase equilibrium. Development of the experimental methodology for the characterization of slag–matte phase equilibrium is presented in the paper. Following a detailed analysis of the potential reaction pathways, experimental techniques have been developed that enable accurate measurement of slag–matte phase equilibrium in the Pb-Fe-O-S-Si system. The application of the techniques has been demonstrated for two important sets of conditions: (i) Condensed phase equilibrium for the slag–matte–metal–tridymite subsystem; and (ii) Gas–slag–matte–tridymite equilibrium at fixed oxygen and sulfur partial pressures. The experimental methodology involves high-temperature equilibration of synthetic samples, fast quenching, and microanalysis of the compositions of phases using electron probe X-ray microanalysis (EPMA). The experimental results are not affected by the changes in the bulk composition of samples during equilibration; this helps to overcome the significant barriers previously encountered in undertaking accurate measurement and characterization of these systems.
Determination of Electrogenic Potential and Removal of Organic Matter from Industrial Coffee Wastewater Using a Native Community in a Non-Conventional Microbial Fuel Cell
Authors: Erazo Santiago , Agudelo-Escobar Lina María
Published: January 2023

Microbial fuel cells (MFCs) are an alternative to conventional wastewater treatments that allow for the removal of organic matter and cogeneration of electrical energy, taking advantage of the oxidation–reduction metabolism of organic compounds conducted by microorganisms. In this study, the electrogenic potential and the capacity for the reduction of the organic matter of native microbial communities in wastewater from the wet processing of coffee were evaluated using open-cathode MFCs. To determine the electrogenic potential, a factorial experimental design was proposed in which the origin of the residual water and the source of the inoculum were evaluated as factors. The MFCs operated for 21 days in both open-circuit and closed-circuit operation modes. Voltage records, current determinations, and chemical oxygen demand (COD) analyses were used to establish the power reached in the electrochemical system and the degree of the decontamination of the wastewater. During the MFC operation, voltages from 200–400 mV and power and current densities from 300–900 mW·m−2 and 10–22 mA·m−2, respectively, were reached. The inoculum used, with a statistical significance of α < 0.05, influenced the electrogenic performance of the microbial fuel cell. The previous process of adaptation to the operational conditions of the MFCs of the native microbial community positively influenced the current generation in the system. The degradation rates reached 500–600 mg·L−1·day−1, indicating the metabolic capacity of the microbial community in the MFCs to achieve the decontamination of wastewater from the coffee agroindustry. It was shown the implementation of bioelectrochemical systems constituted a viable option for the treatment of agricultural waste in Colombia. In addition, it was observed the capacity to cogenerate electrical energy from the biotransformation of the polluting organic matter in the effluents of the coffee industry.
Perceived Risks of Autonomous Vehicles
Authors: Lazányi Kornélia
Published: January 2023

Whilst self-driving cars are not vehicles of the future, but technology that is already available, their acceptance and implementation is heavily limited. People consider them as technology that has a lot of risk—be it technological, IT related, or even ethical. The aim of the present paper is to enrich the existing body of literature of risk perception—and in line with this technology adaption—regarding autonomous vehicles and how they are influenced by demographic and exogenous cultural variables. Whilst the effect of cultural variables on risk perception has already been explored by several researchers, the present paper shall be considered an expansion of those works, striving to address a particular segment of risk perception—the specifics of cultural influence on risk perception regarding autonomous vehicles. Whilst risk perception is of a multifaceted nature, the current paper does not aim to provide a comprehensive understanding of the complex phenomenon under scrutiny, but intends to highlight the potentiality of cultural influences besides the often-explored individual variables when it comes to risk perception and the consequent decisions and indicates that the cultural dimensions of Geert Hofstede use to create a better understanding of perceived risks related to self-driving cars.
Optimal Design and Experimental Validation of a Cable-Driven Parallel Robot for Movement Training of the Head–Neck Joint
Authors: Koszulinski Alizée , Ennaiem Ferdaws , Sandoval Juan , Romdhane Lotfi , Laribi Med Amine
Published: January 2023

The optimal design and the experimental validation of a cable-driven parallel robot is discussed in this paper. This novel device is intended for assisting therapists in the rehabilitation of the head–neck joint. First, the motion of the head–neck joint was recorded by means of a Qualisys motion-capture system. The collected data was then analyzed in order to obtain the range of motion of the head and to identify some prescribed trajectories. A novel end-effector design was proposed to reduce the degrees of freedom needed to perform the desired head motion. The best design was found through an optimization problem where the cable tensions are minimized and the robot dexterity is maximized within its workspace. The objective of this optimization was to find the optimal actuator positions while satisfying a set of constraints. A prototype was proposed and experimental tests were conducted to validate the robot performance. In particular, a comparison between the actual trajectories and the desired ones was performed. The experimental test showed promising results.
Fashion-Oriented Image Captioning with External Knowledge Retrieval and Fully Attentive Gates
Authors: Moratelli Nicholas , Barraco Manuele , Morelli Davide , Cornia Marcella , Baraldi Lorenzo , Cucchiara Rita
Published: January 2023

Research related to fashion and e-commerce domains is gaining attention in computer vision and multimedia communities. Following this trend, this article tackles the task of generating fine-grained and accurate natural language descriptions of fashion items, a recently-proposed and under-explored challenge that is still far from being solved. To overcome the limitations of previous approaches, a transformer-based captioning model was designed with the integration of external textual memory that could be accessed through k-nearest neighbor (kNN) searches. From an architectural point of view, the proposed transformer model can read and retrieve items from the external memory through cross-attention operations, and tune the flow of information coming from the external memory thanks to a novel fully attentive gate. Experimental analyses were carried out on the fashion captioning dataset (FACAD) for fashion image captioning, which contains more than 130k fine-grained descriptions, validating the effectiveness of the proposed approach and the proposed architectural strategies in comparison with carefully designed baselines and state-of-the-art approaches. The presented method constantly outperforms all compared approaches, demonstrating its effectiveness for fashion image captioning.
Development of a Machine-Learning Intrusion Detection System and Testing of Its Performance Using a Generative Adversarial Network
Authors: Mari Andrei-Grigore , Zinca Daniel , Dobrota Virgil
Published: January 2023

Intrusion detection and prevention are two of the most important issues to solve in network security infrastructure. Intrusion detection systems (IDSs) protect networks by using patterns to detect malicious traffic. As attackers have tried to dissimulate traffic in order to evade the rules applied, several machine learning-based IDSs have been developed. In this study, we focused on one such model involving several algorithms and used the NSL-KDD dataset as a benchmark to train and evaluate its performance. We demonstrate a way to create adversarial instances of network traffic that can be used to evade detection by a machine learning-based IDS. Moreover, this traffic can be used for training in order to improve performance in the case of new attacks. Thus, a generative adversarial network (GAN)—i.e., an architecture based on a deep-learning algorithm capable of creating generative models—was implemented. Furthermore, we tested the IDS performance using the generated adversarial traffic. The results showed that, even in the case of the GAN-generated traffic (which could successfully evade IDS detection), by using the adversarial traffic in the testing process, we could improve the machine learning-based IDS performance.
A Novel Fluorescent Aptamer Sensor with DNAzyme Signal Amplification for the Detection of CEA in Blood
Authors: Wei Qingmin , Huang Huakui , Wang Shulong , Liu Fa , Xu Jiayao , Luo Zhihui
Published: January 2023

Carcinoembryonic antigen (CEA) is a tumor-specific biomarker; however, its low levels in the early stages of cancer make it difficult to detect. To address the need for analysis of ultra-low-level substances, we designed and synthesized a fluorescent aptamer sensor with DNAzyme signal amplification and used it for the detection of CEA in blood. In the presence of the target protein, the aptamer sequence in the recognition probe binds to the target protein and opens the hairpin structure, hybridizes with the primer and triggers a polymerization reaction in the presence of polymerase to generate double-stranded DNA with two restriction endonuclease Nb.BbvCl cleavage sites. At the same time, the target protein is displaced and continues to bind to another recognition probe, triggering a new round of polymerization reaction, forming a cyclic signal amplification triggered by the target. The experimental results show that the blood detection with CEA has a high sensitivity and a wide detection range. The detection range: 10 fg/mL~10 ng/mL, with a detection limit of 5.2 fg/mL. In addition, the sensor can be used for the analysis of complex biological samples such as blood.
A Prototype for a Passive Resonant Interferometric Fiber Optic Gyroscope with a 3 × 3 Directional Coupler
Authors: Ovchinnikov Konstantin A. , Gilev Daniil G. , Krishtop Victor V. , Volyntsev Anatoliy B. , Maximenko Vitaliy A. , Garkushin Alexey A. , Filatov Yurii V. , Kukaev Alexander S. , Sevryugin Alexander A. , Shalymov Egor V. , Venediktova Anastasiya V. , Venediktov Vladimir Yu.
Published: January 2023

Reducing the dimensions of optical gyroscopes is a crucial task and resonant fiber optic gyroscopes are promising candidates for its solution. The paper presents a prototype of a miniature resonant interferometric gyroscope of a strategic accuracy class. Due to the use of passive optical elements in this gyroscope, it has a great potential for miniaturization, alongside a low production cost and ease of implementation, since it does not require many feedback loops. The presented prototype shows results on a zero instability of 20°/h and an angle random walk of 0.16°/√h. A theoretical model explaining the nature of the multipath interference of resonant spectra and establishing the relationship between the resonator parameters and the output parameters of the presented prototype is proposed. The results predicted are in agreement with the experimental data. The prototype gyroscope demonstrates a scale factor instability and a change in the average signal level, which is due to the presence of polarization non-reciprocity, occurring due to the induced birefringence in the single-mode fiber of the contour. This problem requires further investigation to be performed.