bluuebunny/crossref_metadata_2025 huggingface dataset:
Iron and Iodine Status in Pregnant Women from A Developing Country and Its Relation to Pregnancy Outcomes
Authors: Iqbal Sehar , Rust Petra , Weitensfelder Lisbeth , Ali Inayat , Kundi Michael , Moshammer Hanns , Ekmekcioglu Cem
Published: November 2019

Birth related complications and comorbidities are highly associated with a poor nutritional status of pregnant women, whereas iron and iodine are among especially important trace elements for healthy maternal and fetal outcomes. The study compares the status of iron, iodine, and related functional parameters in pregnant and non-pregnant women from a developing country and associates the data with pregnancy complications. The concentrations of ferritin, hemoglobin (Hb), total triiodothyronine (TT3), total thyroxine (TT4), and thyroid-stimulating hormone (TSH) were determined in the blood serum of 80 pregnant women at the time of delivery and compared with 40 non-pregnant healthy controls. Spot urine samples were taken to evaluate the urinary iodine concentration (UIC). In pregnant women, ferritin, Hb concentrations, and UIC were significantly lower, and TT4 values were significantly higher compared to controls. Higher Hb levels were tendentially associated with a reduced risk for pregnancy complications (OR = 0.747, CI (95%) 0.556–1.004; p = 0.053). Regarding covariates, only previous miscarriages were marginally associated with pregnancy complications. High consumption of dairy products was associated with lower Hb and ferritin values. Our results suggest that pregnant women from a developing country have lower iron status with Hb levels being possibly associated with pregnancy complications.
Pediatric Healthcare Utilization in a Large Cohort of Refugee Children Entering Western Europe During the Migrant Crisis
Authors: Happle Christine , Dopfer Christian , Ernst Diana , Kleinert Evelyn , Vakilzadeh Annabelle , Hellms Susanne , Evlampidou Iro , Hillermann Nele , Schmidt Reinhold E. , Behrens Georg M. N. , Müller Frank , Wetzke Martin , Jablonka Alexandra
Published: November 2019

Background: Currently, half of the population displaced worldwide is children and adolescents. Little is known on healthcare demand in underage migrants. Materials and Methods: We analyzed healthcare utilization in n = 1.411 children and adolescents living in a large German refugee reception in 2015-2016. Results: The mean age of all included refugees was 9 years (60.8% male). The majority came from the eastern Mediterranean region. During a mean camp inhabitance of 34 days, 57.6% needed primary healthcare, with a significant inverse correlation of healthcare seeking frequency with age and duration of camp inhabitance. Infants and unaccompanied minors displayed particular high demands for medical help. Discussion: Our analysis showed that pediatric primary healthcare in pediatric and adolescent refugees are most sought during the first period upon arrival with particular demand in infants, toddlers, and unaccompanied minors. Based on this data, future care taking strategies should be adapted accordingly.
State-of-the-Science Review of Non-Chemical Stressors Found in a Child’s Social Environment
Authors: Hibbert Kathleen , Tulve Nicolle S.
Published: November 2019

Background: Children are exposed to chemical and non-chemical stressors from their built, natural, and social environments. Research is needed to advance our scientific understanding of non-chemical stressors, evaluate how they alter the biological response to a chemical stressor, and determine how they impact children’s health and well-being. To do this, we conducted a state-of-the-science review of non-chemical stressors found in a child’s social environment. Methods: Studies eligible for inclusion in this review were identified through a search of the peer-reviewed literature using PubMed and PsycINFO. Combinations of words associated with non-chemical stressors and children were used to form search strings. Filters were used to limit the search to studies published in peer-reviewed journals from 2000–2016 and written in English. Publications found using the search strings and filters went through two rounds of screening. Results: A total of 146 studies met the inclusion criteria. From these studies, 245 non-chemical stressors were evaluated. The non-chemical stressors were then organized into 13 general topic areas: acculturation, adverse childhood experiences, economic, education, family dynamics, food, greenspace, neighborhood, social, stress, urbanicity, violence, and other. Additional information on health outcomes, studies evaluating both chemical and non-chemical stressors, and animal studies are provided. This review provides evidence that non-chemical stressors found in a child’s social environment do influence their health and well-being in both beneficial (e.g., salutatory effects of greenspace and social support) and adverse (e.g., poor relationships between health and selected non-chemical stressors such as economics, educational attainment, exposure to violence, stress) ways. Conclusions: This literature review identified a paucity of studies addressing the combined effects of chemical and non-chemical stressors and children’s health and well-being. This literature review was further complicated by inconsistencies in terminology, methodologies, and the value of non-chemical stressor research in different scientific disciplines. Despite these limitations, this review showed the importance of considering non-chemical stressors from a child’s social environment when addressing children’s environmental health considerations.
Mindfulness-Based IARA Model® Proves Effective to Reduce Stress and Anxiety in Health Care Professionals. A Six-Month Follow-Up Study
Authors: Barattucci Massimiliano , Padovan Anna Maria , Vitale Ermanno , Rapisarda Venerando , Ramaci Tiziana , De Giorgio Andrea
Published: November 2019

Changes in the health care environment, together with specific work-related stressors and the consequences on workers’ health and performance, have led to the implementation of prevention strategies. Among the different approaches, those which are mindfulness-based have been institutionally recommended with an indication provided as to their effectiveness in the management of stress. The aim of the present study was to analyze the efficacy of the mindfulness-based IARA Model® (an Italian acronym translatable into meeting, compliance, responsibility, autonomy) in order to ameliorate perceived stress, anxiety and enhance emotional regulation among health care professionals (HCPs; i.e., doctors, nurses, and healthcare assistants). Four hundred and ninety-seven HCPs, 215 (57.2%) of which were women, were randomly assigned to a mindfulness-based training or control group and agreed to complete questionnaires on emotion regulation difficulties (DERS), anxiety, and perceived stress. Results showed that HCPs who attended the IARA training, compared to the control group, had better emotional regulation, anxiety and stress indices after 6 months from the end of the intervention. Furthermore, the results confirmed the positive relationship between emotional regulation, perceived stress and anxiety. The present study contributes to literature by extending the effectiveness of IARA in improving emotional regulation and well-being in non-clinical samples. Moreover, the study provides support for the idea that some specific emotional regulation processes can be implicated in perceived stress and anxiety. From the application point of view, companies should invest more in stress management intervention, monitoring and training, in order to develop worker skills, emotional self-awareness, and relational resources.
Designing Urban Green Spaces for Older Adults in Asian Cities
Authors: Tan Zheng , Lau Kevin Ka-Lun , Roberts Adam Charles , Chao Stessa Tzu-Yuan , Ng Edward
Published: November 2019

Elderly populations in Asian countries are expected to increase rapidly in the next few decades. Older adults, particularly in high-density cities, spend a considerable amount of time in urban green spaces (UGSs). The World Health Organization noted that UGSs are key to improving the age-friendliness of neighborhoods. Thus, it is necessary to design UGSs for the promotion of healthy ageing to enhance preventive healthcare and relieve medical burdens. This study conducted interviews using a questionnaire with a sample size of 326 participants in the cities of Hong Kong (China) and Tainan (Taiwan region). The inter-relationships among the design of UGSs (e.g., spatial distribution and accessibility, characteristics of plants and UGSs), older adults’ perceptions on safety and aesthetics quality of UGSs, and their self-reported health conditions (assessed by the self-reported SF-12v2 Health Survey) were investigated with bivariate Spearman rank correlation tests. The results indicate that the duration of visits to UGSs was positively associated with mental health and social functioning, two subscales evaluating health-related quality of life in SF 12v2. The statistical model (moderation analysis) showed that such a correlation was especially significant in women and those with low social support and social capital. A positive relationship was found between the physical health subscale and perceived safety in UGSs. This relationship was stronger among older adults living alone (moderation analysis). Furthermore, the color of plants and maintenance condition of UGSs were significant aspects affecting the subjective assessment of aesthetic quality. This study provides useful information regarding how to plan and design urban green spaces with certain characteristics that could improve the accessibility and aesthetic quality, which are preferred by older adults.
A Pill in the Lifeworld of Women in Burkina Faso: Can Misoprostol Reframe the Meaning of Abortion
Authors: Drabo Seydou
Published: November 2019

In Burkina Faso, induced abortion is socially stigmatized, condemned, disapproved and legally restricted to cases of rape, incest, fetal malformation or endangerment to the life of the mother. Many women often resort to unsafe procedures to induce abortion, which puts their health at great risk. Misoprostol, which is officially restricted to the treatment of postpartum hemorrhage or post-abortion care, is also used illegally by women to terminate their pregnancies. Misoprostol represents an addition to the existing abortion methods, such as vacuum aspiration, which health workers have often used to induce abortion clandestinely. Many women also use misoprostol to self-induce abortions, replacing abortifacients such as herbal teas, potions, high doses of antimalarial drugs, or bleach. Despite the changes that occur in abortion access due to the use of misoprostol, little is known about what the drug means to its users and how this meaning can in turn influence the meaning of abortion. The aim of this paper is to describe how the use of misoprostol to terminate pregnancy contributes to changing women’s perception of the meaning of abortion. This paper is based on ethnographic fieldwork conducted between March 2016 and February 2017 in the city of Ouagadougou, Burkina Faso. By examining the relation between the use of misoprostol and the meaning that women give to abortion, this study found that women experience abortion either spontaneously or using emergency contraception with misoprostol. Through the experience of women, this paper claims that the meaning of abortion should be seen as a social construct and fundamentally rooted in individual practices and experiences rather than being subject to dichotomist global discourse.
Incidence Rate and Predictors of Globus Pallidus Necrosis after Charcoal Burning Suicide
Authors: Ku Chung-Hsuan , Huang Wen-Hung , Hsu Ching-Wei , Chen Yu-Chin , Hou Yi-Chou , Wang I-Kuan , Hong Hsiang-Hsi , Wang Yen-Li , Weng Cheng-Hao , Yen Tzung-Hai
Published: November 2019

Objective: This study examined predictors of globus pallidus necrosis as there was a paucity of literature of globus pallidus necrosis resulted from carbon monoxide poisoning after charcoal burning suicide. Methods: A total of 67 patients who had attempted charcoal burning suicide were recruited and stratified into two subgroups based on either presence (n = 40) or absence (n = 27) of globus pallidus necrosis. Demographic, clinical, laboratory, and radiographic data were obtained for cross-sectional analysis. All patients were followed to investigate the risks for mortality. Results: The patients aged 36.8 ± 11.1 years (67.2%) were male. Patients with globus pallidus necrosis were younger (p = 0.044) and had less hypertension (p = 0.015) than patients without globus pallidus necrosis. Furthermore, patients with globus pallidus necrosis suffered from severer medical complications, i.e., fever (p = 0.008), acute myocardial injury (p = 0.022), acute rhabdomyolysis (p = 0.022), and neuropsychiatric symptoms (p < 0.001) than patients without globus pallidus necrosis. Moreover, patients with globus pallidus necrosis received less hyperbaric oxygen therapy than without necrosis (p = 0.024). Two patients (3.0%) died on arrival. In a multivariable regression model, it was revealed that acute myocardial injury (odds ratio 4.6, confidence interval 1.1–18.9, p = 0.034) and neuropsychiatric symptoms (odds ratio 8.0, confidence interval 2.0–31.4, p = 0.003), decreased blood bicarbonate level (odds ratio 0.8, confidence interval 0.7–1.0, p = 0.032), and younger age (odds ratio 0.9, confidence interval 0.9–1.0, p = 0.038) were significant predictors for globus pallidus necrosis. Conclusion: Although patients who had attempted charcoal burning suicide had a low mortality rate (3.0%), globus pallidus necrosis was not uncommon (59.7%) in this population. Further studies are warranted.
Adaptive Choropleth Mapper: An Open-Source Web-Based Tool for Synchronous Exploration of Multiple Variables at Multiple Spatial Extents
Authors: Han Su Yeon , Rey Sergio , Knaap Elijah , Kang Wei , Wolf Levi
Published: November 2019

Choropleth mapping is an essential visualization technique for exploratory spatial data analysis. Visualizing multiple choropleth maps is a technique that spatial analysts use to reveal spatiotemporal patterns of one variable or to compare the geographical distributions of multiple variables. Critical features for effective exploration of multiple choropleth maps are (1) automated computation of the same class intervals for shading different choropleth maps, (2) dynamic visualization of local variation in a variable, and (3) linking for synchronous exploration of multiple choropleth maps. Since the 1990s, these features have been developed and are now included in many commercial geographic information system (GIS) software packages. However, many choropleth mapping tools include only one or two of the three features described above. On the other hand, freely available mapping tools that support side-by-side multiple choropleth map visualizations are usually desktop software only. As a result, most existing tools supporting multiple choropleth-map visualizations cannot be easily integrated with Web-based and open-source data visualization libraries, which have become mainstream in visual analytics and geovisualization. To fill this gap, we introduce an open-source Web-based choropleth mapping tool called the Adaptive Choropleth Mapper (ACM), which combines the three critical features for flexible choropleth mapping.
Ranking of Illegal Buildings Close to Rivers: A Proposal, Its Implementation and Preliminary Validation
Authors: Felice Paolino Di
Published: November 2019

Illegal buildings (IBs) are a dramatic problem in developing countries due to the population explosion, but, at the same time, they represent an unsolved issue in several states usually called advanced (as, for instance, Italy). To protect the environment, and hence, people, land authorities must respond to the challenge of IBs by demolishing them. However, in countries where the phenomenon is extended, it is indispensable to provide those figures with an IT tool that returns to them an order of demolition. Through remote sensing methods, suspicious buildings can be identified with a good approximation, but they are all ex aequo. The research summarized in this paper formalizes a two-steps method to deal with a specific category of IBs, namely, those that are close to rivers. These buildings are of special interest to land authorities because people living or simply working inside them are exposed to the flood hazard that each year claims many victims all over the world. The first step of the method computes the census of the IBs located close to rivers, while the second step computes the ranking of these buildings. The ranking may be used as the IBs demolition order. In the paper, it is also proposed the structure of a Spatial DataBase (briefly, SDB) that is suitable to store the input data necessary to solve the problem, as well as the final ranking. Spatial SQL queries against the SDB implement the proposed two-steps method. A real case study was carried out to make a preliminary validation of the method.
Using Landsat OLI and Random Forest to Assess Grassland Degradation with Aboveground Net Primary Production and Electrical Conductivity Data
Authors: Yu Hao , Wang Lei , Wang Zongming , Ren Chunying , Zhang Bai
Published: November 2019

Grassland coverage, aboveground net primary production (ANPP), and species composition are used as indicators of grassland degradation. However, soil salinization deficiency, which is also a factor of grassland degradation, is rarely used in grassland degradation assessment in semiarid regions. We assessed grassland degradation by its quality, quantity, and spatial pattern over semiarid west Jilin, China. Considering soil salinization in west Jilin, electrical conductivity (EC) is used as an index with ANPP to assess grassland degradation. First, the spatial distribution of the grassland was measured with information mined from multi-temporal remote sensing images using an object-based image analysis combined with classification and decision tree methods. Second, with 166 field samples, we utilized the random forest (RF) algorithm as the variable selection and regression method for predicting EC and ANPP. Finally, we created a new grassland degradation model (GDM) based on ANPP and EC. The results showed the R2 (0.91) and RMSE (0.057 mS/cm) of the EC model were generally highest and lowest when the ntree was 400; the ANPP model was optimal (R2 = 0.85 and RMSE = 15.81 gC/m2) when the ntree was 600. Grassland area of west Jilin was 609.67 × 103 ha in 2017, there were 373.79 × 103 ha of degraded grassland, with 210.47 × 103 ha being intensively degraded. This paper surpasses past limitations of excessive reliance on vegetation index to construct a grassland degradation model which considers the characteristics of the study area and soil salinity. The results confirm the positive influence of the ecological conservation projects sponsored by the government. The research outcome could offer supporting data for decision making to help alleviate grassland degradation and promote the rehabilitation of grassland vegetation.
Diesel Exhaust Particle Exposure Compromises Alveolar Macrophage Mitochondrial Bioenergetics
Authors: Gibbs Jonathan L. , Dallon Blake W. , Lewis Joshua B. , Walton Chase M. , Arroyo Juan A. , Reynolds Paul R. , Bikman Benjamin T.
Published: November 2019

Diesel exhaust particles (DEPs) are known pathogenic pollutants that constitute a significant quantity of air pollution. Given the ubiquitous presence of macrophages throughout the body, including the lungs, as well as their critical role in tissue and organismal metabolic function, we sought to determine the effect of DEP exposure on macrophage mitochondrial function. Following daily DEP exposure in mice, pulmonary macrophages were isolated for mitochondrial analyses, revealing reduced respiration rates and dramatically elevated H2O2 levels. Serum ceramides and inflammatory cytokines were increased. To determine the degree to which the changes in mitochondrial function in macrophages were not dependent on any cross-cell communication, primary pulmonary murine macrophages were used to replicate the DEP exposure in a cell culture model. We observed similar changes as seen in pulmonary macrophages, namely diminished mitochondrial respiration, but increased H2O2 production. Interestingly, when treated with myriocin to inhibit ceramide biosynthesis, these DEP-induced mitochondrial changes were mitigated. Altogether, these data suggest that DEP exposure may compromise macrophage mitochondrial and whole-body function via pathologic alterations in macrophage ceramide metabolism.
Druggable Biochemical Pathways and Potential Therapeutic Alternatives to Target Leukemic Stem Cells and Eliminate the Residual Disease in Chronic Myeloid Leukemia
Authors: Muselli Fabien , Peyron Jean-François , Mary Didier
Published: November 2019

Chronic Myeloid Leukemia (CML) is a disease arising in stem cells expressing the BCR-ABL oncogenic tyrosine kinase that transforms one Hematopoietic stem/progenitor Cell into a Leukemic Stem Cell (LSC) at the origin of differentiated and proliferating leukemic cells in the bone marrow (BM). CML-LSCs are recognized as being responsible for resistances and relapses that occur despite the advent of BCR-ABL-targeting therapies with Tyrosine Kinase Inhibitors (TKIs). LSCs share a lot of functional properties with Hematopoietic Stem Cells (HSCs) although some phenotypical and functional differences have been described during the last two decades. Subverted mechanisms affecting epigenetic processes, apoptosis, autophagy and more recently metabolism and immunology in the bone marrow microenvironment (BMM) have been reported. The aim of this review is to bring together the modifications and molecular mechanisms that are known to account for TKI resistance in primary CML-LSCs and to focus on the potential solutions that can circumvent these resistances, in particular those that have been, or will be tested in clinical trials.
Differences in Osteoimmunological Biomarkers Predictive of Psoriatic Arthritis among a Large Italian Cohort of Psoriatic Patients
Authors: Diani Marco , Perego Silvia , Sansoni Veronica , Bertino Lucrezia , Gomarasca Marta , Faraldi Martina , Pigatto Paolo Daniele Maria , Damiani Giovanni , Banfi Giuseppe , Altomare Gianfranco , Lombardi Giovanni
Published: November 2019

(1) Background: In literature it is reported that 20–30% of psoriatic patients evolve to psoriatic arthritis over time. Currently, no specific biochemical markers can either predict progression to psoriatic arthritis or response to therapies. This study aimed to identify osteoimmunological markers applicable to clinical practice, giving a quantitative tool for evaluating pathological status and, eventually, to provide prognostic support in diagnosis. (2) Methods: Soluble (serum) bone and cartilage markers were quantified in 50 patients with only psoriasis, 50 psoriatic patients with psoriatic arthritis, and 20 healthy controls by means of multiplex and enzyme-linked immunoassays. (3) Results: Differences in the concentrations of matrix metalloproteases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), receptor activator of nuclear factor kappa-B- ligand (RANK-L), procollagen type I N propeptide (PINP), C-terminal telopeptide of type I collagen (CTx-I), dickkopf-related protein 1 (DKK1), and sclerostin (SOST) distinguished healthy controls from psoriasis and psoriatic arthritis patients. We found that MMP2, MMP12, MMP13, TIMP2, and TIMP4 distinguished psoriasis from psoriatic arthritis patients undergoing a systemic treatment, with a good diagnostic accuracy (Area under the ROC Curve (AUC) > 0.7). Then, chitinase-3-like protein 1 (CHI3L1) and MMP10 distinguished psoriasis from psoriatic arthritis not undergoing systemic therapy and, in the presence of onychopathy, MMP8 levels were higher in psoriasis than in psoriatic arthritis. However, in these latter cases, the diagnostic accuracy of the identified biomarkers was low (0.5 < AUC < 0.7). (4) Conclusions. By highlighting never exploited differences, the wide osteoimmunological biomarkers panel provides a novel clue to the development of diagnostic paths in psoriasis and psoriasis-associated arthropathic disease.
Transcription Factor Prospero Homeobox 1 (PROX1) as a Potential Angiogenic Regulator of Follicular Thyroid Cancer Dissemination
Authors: Rudzińska Magdalena , Mikula Michał , Arczewska Katarzyna D. , Gajda Ewa , Sabalińska Stanisława , Stępień Tomasz , Ostrowski Jerzy , Czarnocka Barbara
Published: November 2019

It is well known that Prospero homeobox 1 (PROX1) is a crucial regulator of lymphangiogenesis, that reprograms blood endothelial cells to lymphatic phenotype. However, the role of PROX1 in tumor progression, especially in angiogenesis remains controversial. Herein, we studied the role of PROX1 in angiogenesis in cell lines derived from follicular thyroid cancer (FTC: FTC-133) and squamous cell carcinoma of the thyroid gland (SCT: CGTH-W-1) upon PROX1 knockdown. The genes involved in angiogenesis were selected by RNA-seq, and the impact of PROX1 on vascularization potential was investigated using human umbilical vein endothelial cells (HUVECs) cultured in conditioned medium collected from FTC- or SCT-derived cancer cell lines after PROX1 silencing. The angiogenic phenotype was examined in connection with the analysis of focal adhesion and correlated with fibroblast growth factor 2 (FGF2) levels. Additionally, the expression of selected genes involved in angiogenesis was detected in human FTC tissues. As a result, we demonstrated that PROX1 knockdown resulted in upregulation of factors associated with vascularization, such as metalloproteinases (MMP1 and 3), FGF2, vascular endothelial growth factors C (VEGFC), BAI1 associated protein 2 (BAIAP2), nudix hydrolase 6 (NUDT6), angiopoietin 1 (ANGPT1), and vascular endothelial growth factor receptor 2 (KDR). The observed molecular changes resulted in the enhanced formation of capillary-like structures by HUVECs and upregulated focal adhesion in FTC-133 and CGTH-W-1 cells. The signature of selected angiogenic genes’ expression in a series of FTC specimens varied depending on the case. Interestingly, PROX1 and FGF2 showed opposing expression levels in FTC tissues and seven thyroid tumor-derived cell lines. In summary, our data revealed that PROX1 is involved in the spreading of thyroid cancer cells by regulation of angiogenesis.
Epinephrine Infiltration of Adipose Tissue Impacts MCF7 Breast Cancer Cells and Total Lipid Content
Authors: Avril Pierre , Vidal Luciano , Barille-Nion Sophie , Le Nail Louis-Romée , Redini Françoise , Layrolle Pierre , Pinault Michelle , Chevalier Stéphane , Perrot Pierre , Trichet Valérie
Published: November 2019

Background: Considering the positive or negative potential effects of adipocytes, depending on their lipid composition, on breast tumor progression, it is important to evaluate whether adipose tissue (AT) harvesting procedures, including epinephrine infiltration, may influence breast cancer progression. Methods: Culture medium conditioned with epinephrine-infiltrated adipose tissue was tested on human Michigan Cancer Foundation-7 (MCF7) breast cancer cells, cultured in monolayer or in oncospheres. Lipid composition was evaluated depending on epinephrine-infiltration for five patients. Epinephrine-infiltrated adipose tissue (EI-AT) or corresponding conditioned medium (EI-CM) were injected into orthotopic breast carcinoma induced in athymic mouse. Results: EI-CM significantly increased the proliferation rate of MCF7 cells Moreover EI-CM induced an output of the quiescent state of MCF7 cells, but it could be either an activator or inhibitor of the epithelial mesenchymal transition as indicated by gene expression changes. EI-CM presented a significantly higher lipid total weight compared with the conditioned medium obtained from non-infiltrated-AT of paired-patients. In vivo, neither the EI-CM or EI-AT injection significantly promoted MCF7-induced tumor growth. Conclusions: Even though conditioned media are widely used to mimic the secretome of cells or tissues, they may produce different effects on tumor progression, which may explain some of the discrepancy observed between in vitro, preclinical and clinical data using AT samples.
Cloning and Expression Analysis of the BocMBF1c Gene Involved in Heat Tolerance in Chinese Kale
Authors: Zou Lifang , Yu Bingwei , Ma Xing-Liang , Cao Bihao , Chen Guoju , Chen Changming , Lei Jianjun
Published: November 2019

Chinese kale (Brassica oleracea var. chinensis Lei) is an important vegetable crop in South China, valued for its nutritional content and taste. Nonetheless, the thermal tolerance of Chinese kale still needs improvement. Molecular characterization of Chinese kale’s heat stress response could provide a timely solution for developing a thermally tolerant Chinese kale variety. Here, we report the cloning of multi-protein bridging factor (MBF) 1c from Chinese kale (BocMBF1c), an ortholog to the key heat stress responsive gene MBF1c. Phylogenetic analysis showed that BocMBF1c is highly similar to the stress-response transcriptional coactivator MBF1c from Arabidopsis thaliana (AtMBF1c), and the BocMBF1c coding region conserves MBF1 and helix-turn-helix (HTH) domains. Moreover, the promoter region of BocMBF1c contains three heat shock elements (HSEs) and, thus, is highly responsive to heat treatment. This was verified in Nicotiana benthamiana leaf tissue using a green fluorescent protein (GFP) reporter. In addition, the expression of BocMBF1c can be induced by various abiotic stresses in Chinese kale which indicates the involvement of stress responses. The BocMBF1c-eGFP (enhanced green fluorescent protein) chimeric protein quickly translocated into the nucleus under high temperature treatment in Nicotiana benthamiana leaf tissue. Overexpression of BocMBF1c in Arabidopsis thaliana results in a larger size and enhanced thermal tolerance compared with the wild type. Our results provide valuable insight for the role of BocMBF1c during heat stress in Chinese kale.
DNA Methylation-Governed Gene Expression in Autoimmune Arthritis
Authors: Brandt Barbara , Rashidiani Shima , Bán Ágnes , Rauch Tibor A.
Published: November 2019

Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease hallmarked by progressive and irreversible joint destruction. RA pathogenesis is a T cell-regulated and B cell-mediated process in which activated lymphocyte-produced chemokines and cytokines promote leukocyte infiltration that ultimately leads to destruction of the joints. There is an obvious need to discover new drugs for RA treatment that have different biological targets or modes of action than the currently employed therapeutics. Environmental factors such as cigarette smoke, certain diet components, and oral pathogens can significantly affect gene regulation via epigenetic factors. Epigenetics opened a new field for pharmacology, and DNA methylation and histone modification-implicated factors are feasible targets for RA therapy. Exploring RA pathogenesis involved epigenetic factors and mechanisms is crucial for developing more efficient RA therapies. Here we review epigenetic alterations associated with RA pathogenesis including DNA methylation and interacting factors. Additionally, we will summarize the literature revealing the involved molecular structures and interactions. Finally, potential epigenetic factor-based therapies will be discussed that may help in better management of RA in the future.
Precision Agriculture: A Remote Sensing Monitoring System Architecture
Authors: Triantafyllou Anna , Sarigiannidis Panagiotis , Bibi Stamatia
Published: November 2019

Smart Farming is a development that emphasizes on the use of modern technologies in the cyber-physical field management cycle. Technologies such as the Internet of Things (IoT) and Cloud Computing have accelerated the digital transformation of the conventional agricultural practices promising increased production rate and product quality. The adoption of smart farming though is hampered because of the lack of models providing guidance to practitioners regarding the necessary components that constitute IoT-based monitoring systems. To guide the process of designing and implementing Smart farming monitoring systems, in this paper we propose a generic reference architecture model, taking also into consideration a very important non-functional requirement, the energy consumption restriction. Moreover, we present and discuss the technologies that incorporate the seven layers of the architecture model that are the Sensor Layer, the Link Layer, the Encapsulation Layer, the Middleware Layer, the Configuration Layer, the Management Layer and the Application Layer. Furthermore, the proposed Reference Architecture model is exemplified in a real-world application for surveying Saffron agriculture in Kozani, Greece.
A Novel Approach to Working Memory Training Based on Robotics and AI
Authors: Araujo Vladimir , Mendez Diego , Gonzalez Alejandra
Published: November 2019

Working memory is an important function for human cognition since several day-to-day activities are related to it, such as remembering a direction or developing a mental calculation. Unfortunately, working memory deficiencies affect performance in work or education related activities, mainly due to lack of concentration, and, with the goal to improve this, many software applications have been developed. However, sometimes the user ends up bored with these games and drops out easily. To cope with this, our work explores the use of intelligent robotics and dynamic difficulty adjustment mechanisms to develop a novel working memory training system. The proposed system, based on the Nao robotic platform, is composed of three main components: First, the N-back task allows stimulating the working memory by remembering visual sequences. Second, a BDI model implements an intelligent agent for decision-making during the progress of the game. Third, a fuzzy controller, as a dynamic difficulty adjustment system, generates customized levels according to the user. The experimental results of our system, when compared to a computer-based implementation of the N-back game, show a significant improvement on the performance of the user in the game, which might relate to an improvement in their working memory. Additionally, by providing a friendly and interactive interface, the participants have reported a more immersive and better game experience when using the robotic-based system.
Speech Synthesis in the Translation Revision Process: Evidence from Error Analysis, Questionnaire, and Eye-Tracking
Authors: Ciobanu Dragoş , Ragni Valentina , Secară Alina
Published: November 2019

Translation revision is a relevant topic for translator training and research. Recent technological developments justify increased focus on embedding speech technologies—speech synthesis (text-to-speech) and speech recognition (speech-to-text)—into revision workflows. Despite some integration of speech recognition into computer-assisted translation (CAT)/translation environment tools (TEnT)/Revision tools, to date we are unaware of any CAT/TEnT/Revision tool that includes speech synthesis. This paper addresses this issue by presenting initial results of a case study with 11 participants exploring if and how the presence of sound, specifically in the source text (ST), affects revisers’ revision quality, preference and viewing behaviour. Our findings suggest an improvement in revision quality, especially regarding Accuracy errors, when sound was present. The majority of participants preferred listening to the ST while revising, but their self-reported gains on concentration and productivity were not conclusive. For viewing behaviour, a subset of eye-tracking data shows that participants focused more on the target text (TT) than the source regardless of the revising condition, though with differences in fixation counts, dwell time and mean fixation duration (MDF). Orientation and finalisation phases were also identified. Finally, speech synthesis appears to increase perceived alertness, and may prompt revisers to consult external resources more frequently.
Effects of Dual Exposure to the Herbicides Atrazine and Paraquat on Adult Climbing Ability and Longevity in Drosophila melanogaster
Authors: Lovejoy Pamela C. , Fiumera Anthony C.
Published: November 2019

Anthropomorphic effects are changing the planet, and therefore, organisms are being exposed to many new biotic and abiotic stressors. Exposure to multiple stressors can affect organisms in ways that are different than the sum of their individual effects, and these interactions are often difficult to predict. Atrazine and paraquat are two of the most widely used herbicides in the United States, and are individually known to increase oxidative damage, affect dopaminergic functioning, reduce longevity, and alter motor ability in non-target organisms. We measured the effects of individual and combined exposure to low doses of atrazine and paraquat on climbing ability and longevity of Drosophila melanogaster. Atrazine and paraquat interact to affect D. melanogaster climbing ability and longevity in different ways. Atrazine appeared to have a weak mitigative effect against the decrease in climbing ability caused by paraquat. In contrast, combined exposure to atrazine and paraquat had detrimental synergistic effects on female longevity. Overall, this study shows that atrazine and paraquat can interact and that it is important to measure several traits when assessing the consequences of exposure to multiple stressors. Future studies should continue to assess the impacts of stressor interactions on organisms, as many combinations have never been examined.
Bmserpin2 Is Involved in BmNPV Infection by Suppressing Melanization in Bombyx mori
Authors: Toufeeq Shahzad , Wang Jie , Zhang Shang-Zhi , Li Bing , Hu Pei , Zhu Lin-Bao , You Ling-Ling , Xu Jia-Ping
Published: November 2019

Melanization, an important defense response, plays a vital role in arthropod immunity. It is mediated by serine proteases (SPs) that convert the inactive prophenoloxidase (PPO) to active phenoloxidase (PO) and is tightly regulated by serine protease inhibitors (serpins) which belong to a well distributed superfamily in invertebrates, participating in immune mechanisms and other important physiological processes. Here, we investigated the Bmserpin2 gene which was identified from a transcriptome database in response to Bombyx mori nucleopolyhedrovirus (BmNPV) infection. Quantitative real-time polymerase chain reaction (qRT-PCR) results showed that Bmserpin2 was expressed in all tissues, with maximum expression in fat body. Upon BmNPV infection, the expression of Bmserpin2 was up-regulated in P50 (susceptible strain) and BC9 (resistant strain) in haemocytes, fat body and the midgut. However, up-regulation was delayed in BC9 (48 or 72 h), in contrast to P50 (24 h), after BmNPV infection. Meanwhile, Bmserpin2 could delay or inhibit melanization in silkworm haemolymph. Significant increased PO activity can be observed in Bmserpin2-depleted haemolymph under NPV infection. Furthermore, the viral genomic DNA copy number was decreased in Bmserpin2-depleted haemolymph. We conclude that Bmserpin2 is an inducible gene which might be involved in the regulation of PPO activation and suppressed melanization, and have a potential role in the innate immune system of B. mori.
Efficacy of Low Dose Proton Pump Inhibitor-Based Therapy to Eradicate Helicobacter pylori in Patients with Subtotal Gastrectomy
Authors: Kim Dongwoo , Jung Sung Woo , Lee Dong-won , Lee Chang Min , Kim Seung Young , Hyun Jong Jin , Jung Young Kul , Koo Ja Seol , Yim Hyung Joon , Lee Sang Woo
Published: November 2019

Proton pump inhibitor (PPI)-based therapy is standard to eradicate Helicobacter pylori (H. pylori). Gastric acidity is lowered after gastrectomy because of bile reflux and impaired mechanism of acid secretion. Therefore, low-dose PPI may be effective for H. pylori eradication in the remnant stomach after gastrectomy. In this study, we compared the efficacy of low-dose PPI with standard double-dose PPI to eradicate H. pylori in patients who underwent subtotal gastrectomy. A total of 145 patients who were treated for eradication after gastrectomy was analyzed. They were treated with PPI-based triple regimen (PPI, clarithromycin and amoxicillin) for 14 days. We compared the eradication rate in the low-dose PPI group (lansoprazole 15 mg once daily) with that in the standard double-dose PPI group (lansoprazole 30 mg twice daily). The H. pylori eradication rate was 79.1% in the low-dose PPI group and 85.3% in the standard double-dose group; the difference was not significant statistically (p = 0.357). In the multivariate analysis, low-dose PPI (odds ratio (OR) = 1.79, 95% confidence interval (CI), 0.68–4.69) was not associated with eradication failure, while Billroth II anastomosis (OR = 4.45, 85% CI, 1.23–16.2) was significantly associated with eradication failure. Low-dose PPI-based triple regimen was as effective as standard double-dose PPI-based regimen for H. pylori eradication in patients with subtotal gastrectomy. Further study is needed to confirm the effect of low-dose PPI on H. pylori eradication in patients with gastrectomy.
Bacterial Diversity of Diabetic Foot Ulcers: Current Status and Future Prospectives
Authors: Sadeghpour Heravi Fatemah , Zakrzewski Martha , Vickery Karen , G. Armstrong David , Hu Honghua
Published: November 2019

Diabetic foot ulcers (DFUs) and diabetic foot infections (DFIs) are associated with reduced patient quality of life, lower-extremity amputation, hospitalization, and high morbidity and mortality. Diverse bacterial communities have been identified in DFUs/DFIs, playing a significant role in infection prognosis. However, due to the high heterogeneity of bacterial communities colonized in DFUs/DFIs, culture-based methods may not isolate all of the bacterial population or unexpected microorganisms. Recently, high sensitivity and specificity of DNA (metagenomics) and RNA (metatranscriptomics) technologies have addressed limitations of culture-based methods and have taken a step beyond bacterial identification. As a consequence, new advances obtained from DNA- and RNA-based techniques for bacterial identification can improve therapeutic approaches. This review evaluated the current state of play in aetiology of DFUs/DFIs on culture and molecular approaches, and discussed the impact of metagenomic and metatranscriptomic methods in bacterial identification approaches.
Comment from the Editors on the Special Issue: Advanced Analytical Methods in Clinical Diagnosis and Therapy
Authors: Florescu Monica , Rogozea Liliana
Published: November 2019

With this Editorial, we want to present the Special Issue, “Advanced Analytical Methods in Clinical Diagnosis and Therapy”. The development of medicine is not possible without progress in the field of identifying different biomarkers or treatments using modern approaches, such as the analytical methods presented in articles that are part of this issue. Thus, with the support of experts, both aspects of theoretical and practical interest from different fields of pathologies have been addressed.
Characteristics of Mild Cognitive Impairment in Northern Japanese Community-Dwellers from the ORANGE Registry
Authors: Kume Yu , Takahashi Tomoko , Itakura Yuki , Lee Sangyoon , Makizako Hyuma , Ono Tsuyosi , Shimada Hiroyuki , Ota Hidetaka
Published: November 2019

A gradually increasing prevalence of mild cognitive impairment (MCI) is recognized in the super-aging society that Japan faces, and early detection and intervention in community-dwellers with MCI are critical issues to prevent dementia. Although many previous studies have revealed MCI/non-MCI differences in older individuals, information on the prevalence and characteristics of MCI in rural older adults is limited. The aim of this study was to investigate differential characteristics between older adults with and without MCI. The investigation was conducted over one year from 2018 to 2019. Participants were recruited from Akita in northern Japan. Neuropsychological assessments were applied to classify MCI, including the National Center for Geriatrics and Gerontology Functional Assessment Tool (NCGG-FAT) and the Touch panel-type Dementia Assessment Scale (TDAS) based on the Alzheimer’s disease assessment scale. Our samples consisted of 103 older adults divided into 54 non-MCI and 49 MCI. The MCI group had lower scores of all cognitive items. Our results showed that individuals with MCI had significantly slower walking speed (WS) and worse geriatric depression scale (GDS) compared to non-MCI. In addition, WS was significantly associated with some cognitive items in non-MCI, but not in MCI. Finally, we showed that predictive variables of MCI were WS and GDS. Our study provides important information about MCI in rural community-dwellers. We suggest that older adults living in a super-aging society should receive lower limb training, and avoiding depression in older adults through interaction of community-dwellers may contribute to preventing the onset of MCI.
First Evaluation of an Index of Low Vagally-Mediated Heart Rate Variability as a Marker of Health Risks in Human Adults: Proof of Concept
Authors: Jarczok Marc N. , Koenig Julian , Wittling Arne , Fischer Joachim E. , Thayer Julian F.
Published: November 2019

Multiple studies have demonstrated low vagally-mediated heart rate variability (HRV) being associated with a range of risk factors for heart disease and stroke, including inflammation, hyperglycemia, hyperlipidemia, and hypertension. Yet, no cut point exists that indicates elevated risk. In the present study we sought to identify a cut point-value for HRV that is associated with elevated risk across a range of known risk factors. Methods: A total of 9550 working adults from 19 study sites took part in a health assessment that included measures of inflammation, hyperglycemia, hyperlipidemia, and hypertension and vagally-mediated HRV (Root mean square of successive differences between normal heartbeats (RMSSD)). Multiple age and sex adjusted logistic regressions were calculated per risk factor (normal versus clinical range), with RMSSD being entered in binary at different cut points ranging from 15–39 msec with a 2 msec increment. Results: For daytime RMSSD, values below 25 ± 4 indicated elevated risk (odds ratios (OR) 1.5–3.5 across risk factors). For nighttime RMSSD, values below 29 ± 4 indicated elevated risk (OR 1.2–2.0). Conclusion: These results provide the first evidence that a single value of RMSSD may be associated with elevated risk across a range of established cardiovascular risk factors and may present an easy to assess novel marker of cardiovascular risk.
Effect of Cellulose Reducing Ends on the Reinforcing Capacity of Powdered Cellulose in Polypropylene Composites
Authors: Azadfar Mohammadali , Graham Max R. , Wolcott Michael P.
Published: November 2019

Powdered cellulose-reinforced (20 wt%) polypropylene composites were prepared by melt compounding and subsequent injection moldings. We assessed the effect of cellulose reducing ends on the capacity of powdered cellulose to reinforce polypropylene composites after seven days exposure to air circulation during the conditioning of samples. Tensile tests on the composites were performed at 5.08 mm/min. Fourier transform infrared spectroscopy revealed some changes that occurred within the composites by demonstrating a practical decrease in –C=O (1744 cm−1) absorption band intensity. A thermogravimetric analysis indicated differences within the thermal behavior of the prepared composites, showing a higher onset of degradation. Scanning electron microscopy of the fracture areas, together with load–extension curves, further characterized the development of interfacial cellulose/matrix adhesion as well as the brittle and ductile behavior of the composites. The results indicate that the thermal and tensile properties of powdered cellulose/polypropylene are improved by decreasing the amount of cellulose reducing ends in the system.