bluuebunny/crossref_metadata_2025 huggingface dataset:
ULTRA VIOLET VISIBLE INVESTIGATION OF THE OPTIMAL REACTING RATIO FOR TEMPLATE-FUNCTIONAL MONOMER INTERACTION INVOLVING NICOTINE AND

Pleurotus tuberregium FOR MOLECULAR IMPRINTING POLYMERIZATION
Authors: N Adela , O Ofoegbu
Published: January 2023

Molecularly imprinted polymers (MIPs), are materials that has been processed using the molecular imprinting technique. These technology involves three strategies, that is, covalent, non-covalent, and semi-covalent approach (non-covalent approach is commonly used). The most important component for the synthesis of MIPs are template, functional monomer, cross-linkers, initiators and porogenic solvent. Molecularly imprinted polymers have been used in diverse ways, some of which includes; application as polymeric sensors, toxicant selective polymers, drug delivery system (DDS), ocular therapy, Molecular imprinted polymer has been applied in the treatment of contagious tropical infections, and MIP in transdermal therapeutic applications. In this study, an ultra violet visible investigation of the optimal reacting ratio for nicotine and Pleurotus tuberregium was carried out. At 1:5 and 1:6 nicotine and
Pleurotus

tuberregium ratio, the absorbance was 0.352nm and 0.354nm respectively. 1:5 and 1:6 are suitable ratios for template functional monomer interaction due to their stability. Over times, synthetic monomers are commonly used for MIPs, however, more studies should be carried out with biopolymers which are easily available, low cost and easily degradable.
The Effect of Big Data on Firm Value: The Mediation Roles of Innovation and Financial Performances
Authors: Ejrami Mohsen , Salehi Nader
Published: January 2023

Companies that have high growth indicators for liquidity, stock, and return on assets are more desirable to investors and shareholders. Successful managers are thus limited to using financial indicators to convey the value of the firm. Big data can now be the source of innovation and monetary success. Technology, rivals, market and customer data, and these sources of information give businesses hints about emerging competitive attitudes. Therefore, there will be valuable companies that innovate by absorbing new knowledge and data through big data and improving financial performance.
Export Marketing: The Moderator Effects of Dysfunctional Competition
and Institutional Barriers
Authors: Salehi Nader , Kashmari Ali , Shahsavari Haniye
Published: January 2023

In recent decades, companies have become more focused on the export market and cross-border trade. Companies use international expansion to grow, increase profits and sales, maintain a competitive market position, and compensate for the presence of foreign companies in their market. The purpose of this study is to examine the impact of export marketing capabilities and diversification of export entry on export performance, with inefficient competition and organizational barriers acting as moderators. This study is applied, descriptive, and survey-based. The statistical population consists of 67 Niro Motor Industrial Company senior, middle, and production line managers. In this study, the census method was used for sampling. In order to collect data, a questionnaire was extracted from previous studies, and its validity and reliability were confirmed by tests. Structured equation modeling and Smart PLS software were used to analyze the collected data. This study's findings show that greater levels of product innovation and market responsiveness as a result of export marketing capabilities have a significant impact on export performance.
DeutJ: An ImageJ Plugin for Improved Automatic Masking and Segmentation of Images from Confocal Microscopy
Authors: Cui Sunny
Published: January 2023

Due to advances to microscopic imaging, there are a plethora of biological molecules that can now be tagged and subsequentially imaged from almost any cell, organism, or tissue. However, the ability for software to analyze these images remains to be a challenge. ImageJ is an open source software that allows for processing of these images, but faces challenges when dealing with images that show weak contrast between objects of interest and background. DeutJ is an ImageJ plugin that color-corrects and brightness-corrects gradients that inhbit segmentation in confocal microscopy images. It can analyze hundreds of images from a given folder and produces binary mask images that are superior to existing ImageJ functions.
Do Korean Learners of English Use Existential Constructions for the Introduction of New Discourse Referents?
Authors:   The Society Of Modern Grammar, Kim Ji-Hee
Published: January 2023

It is attested that topic prominent L1 learners of English without an advanced level of proficiency, have a strong tendency to use existential constructions to structure the information in sentences in the topic-comment ways of their languages (Lee 2011, Ortega 2009, Callies 2008, Rutherford 1983). Since Korean is a topic prominent language, the current study hypothesized that Korean learners of English would also show such a tendency. To prove this hypothesis, the present study examined how Korean learners of English use there-existentials in their essays. The study results clearly showed that these learners did employ Existential Constructions to package the information of sentences into the frame of topic and comment, but not for introducing new discourse referents.
Word-Order of Clause and NP Objects in Korean: A Corpus Study
Authors:   The Society Of Modern Grammar, Hong EunMi
Published: January 2023

This study aims to identify the canonical word-orders when Korean verbs take two objects: a clause and an NP. To pursue this goal, with over 7,000 tokens of 27 Korean utterance verbs which reportedly possess a ‘hearer-theme’ construction (Cho 2009; Lee 2016), it observes how many objects (zero, one, or two objects) are realized in a token sentence, analyzes in what type of syntactic categories the objects appear, and finally examines which word-order is preferred in case of the two objects in a token. Besides, it attempts to classify the 27 utterance verbs in terms of their object realization, i.e., the number and the syntactic types of objects in the tokens, using K-means clustering algorithm and Heirarchical Clustering. The findings are (i) the most of the data fell into zero or one object cases while only 5% of the data occurred with two objects, (ii) the canonical word-order differed depending on the syntactic type of an NP object so that an Accusative NP mostly followed a clausal object while a Dative NP preferably preceded an object clause, and (iii) the verbs given were best categorized into three groups when they took only one complement: hearer-dominant group, theme-dominant group, and hearer/theme group.
Ministrantura w posoborowej pedagogii Franciszka Blachnickiego (wprowadzenie do problemu)
Authors: Sadowska Katarzyna
Published: January 2023

The aim of the study is to present the formation of altar boys in the post-conciliar pedagogical teaching of priest Franciszek Blachnicki, an outstanding mystagogue of the Second Vatican Council, in the context of Christian education. The post-conciliar documents changed the severity of the Church, pompous and inaccessible to laymen. Concern for the community of the faithful became the dominant idea of the Second Vatican Council. In connection with the changes taking place in the Church and the needs of Catholics in the Polish People’s Republic, Franciszek Blachnicki made an attempt to lay the foundations for pastoral theology. It included the metho-dological assumptions underlying Catholic communities, placed by the priest in the philosophy of the Christocentric tradition, and based on a dialogue derived from “a philosophical meeting”, mutual service and the value of spiritual experience. The work of Franciszek Blachnicki, which remains valid, has made its way to the history of the Polish Catholic Church. While the priest is widely recognized as the founder of the Light – Life movement, he built the foundations for the concept of work for “communion” when altar service was the only communion approved by the Polish Church authorities.
Synthesis of group I–III–VI semiconductor quantum dots and its application in food safety testing
Authors: Zhou Hualan , Wang Huiwen , Li Xiaodi , Wang Lehui , Huang Haoming , Qiu Haojie , Cong Wenjie , Wang Mingxuan , Zhang Jianguo
Published: January 2023

As a good fluorescent material, most I–III–VI ternary semiconductor quantum dots (QDs) do not contain heavy metal elements such as Cd and Pb and thus have low toxicity. In addition, they also have excellent data performance such as narrow band gap, large absorption coefficient, large Stokes shift, low self-absorption effect, and luminescence wavelength in the near infrared region. I–III–VI QDs also have a size-dependent luminescence in the visible and near-infrared ranges, and their fluorescence quantum yields can exceed 50% especially when coated with ZnS shells, thus offering great potential for applications in illumination, display, and bioimaging. The review focuses on the synthesis of group I–III–VI QDs, their properties, and applications. In the first part, we discuss the approaches to synthesize these QDs. In the second section, we provide an overview of the applications of I–III–VI QDs in food safety detection, with emphasis on the detection of heavy metal ions, foodborne pathogens, food additives, pesticide, and veterinary drug residues. The final section summarizes the current application prospects of ternary semiconductor QDs.
Prediction of Soil Moisture Content through Photographs of Cobalt Chloride Filter Paper in Contact with Soil Specimens
Authors: Loshelder Julia I. , Coffman Richard A.
Published: January 2023

Measured soil moisture content is a useful geotechnical engineering property for a variety of analyses. The laboratory determination of soil moisture content can be time consuming, labor intensive, or both; the field determination of soil moisture content may require additional certification, as associated with the use of the nuclear density gauge. The objective of this research was to identify a method that could be used to determine the soil moisture content without the aforementioned disadvantages. This objective was achieved through the use of cobalt chloride filter paper being placed into contact with the soil. The cobalt chloride filter paper changes color in the presence of moisture (a blue color when the paper is dry and a pink color when the paper is wet). A membrane separated the cobalt chloride paper and the surface of soil specimens (kaolinite, bentonite, Donna Fill, and a silty sand) to prevent cobalt chloride migration. The soil specimens were prepared at different moisture contents, and photography-based hue values were determined for each soil/cobalt chloride filter paper pair at given time intervals. Linear correlations were observed to exist between the soil moisture content and the slope of the linear portion of the hue-time relationship for all of the investigated soil types. The coefficients of determination (R2) ranged from .91 to .99. All soils showed a strong positive linear relationship between the slope of the cobalt chloride hue and soil moisture content. This use of hue may provide a rapid determination of in situ soil moisture content.
Interpretation of In Situ State Parameters of Piezocone Penetration Tests in High Pile Rebound Soils
Authors: Shaban Alaa M. , Cosentino Paul J.
Published: January 2023

Cone penetrometer testing (CPT) was performed along with pile driving analysis (PDA) at six piling projects to determine typical soil characteristics encountered during excessive pile rebound. The PDA test was used to develop profiles of rebound versus elevation, while the CPT test was utilized to provide continuous variations of soil parameters with depth. In situ data from the CPT and PDA tests were matched based on depth and elevation, then averaged over four depth intervals ranging from 1B to 8B (B is the pile’s diameter). The results indicated that the rebound and non-rebound CPT data plots clustered in distinctive regions on the soil liquefaction assessment chart. The rebound data showed dilative behavior with state parameter values less than −0.125, while the non-rebound data exhibited contractive behavior, with larger state parameter values. Predictive models for estimating the magnitude of pile rebound in terms of normalized CPT data were thus developed using stepwise linear and nonlinear regression analyses. The results for these showed promising correlations between rebound, pore water pressure, cone tip resistance and state parameter predicted as a function of friction ratio. Additionally, the depth of soil layers underneath the pile’s toe was found to play a prominent role in controlling the extent and the amount of rebound. The CPT data as averaged over 4B and 8B intervals also showed a clear tendency to determine problematic pile rebound zones, though no such tendency was seen in data averaged over 1B and 2B intervals.
PRNP Sequences of Tibetan Antelope, Blue Sheep, and Plateau Pika from the Qinghai-Tibet Plateau and Reactivity of PrP Proteins to Rodent-Adapted Scrapie Strains in RT-QuIC and PMCA
Authors: Wu Yue-Zhang , Wu Jing-Xing , Yang Xue-Hua , Lu Shan , Xiao Kang , Chen Dong-Dong , Gao Li-Ping , Shi Qi , Xu Jian-Guo , Dong Xiao-Ping
Published: January 2023

Background:

Tibetan antelope (Rhinopithecus), blue sheep (Pseudois nayauris), and plateau pika (Ochotona curzoniae) are wild animals living on the Qinghai-Tibet Plateau. There have been no reports of naturally-occurring transmissible spongioform encephalopathies (TSEs) involving these animals. Furthermore, the PRNP genes have not been described in the literature.

Methods:

The PRNP genes from 21 Tibetan antelopes, 4 blue sheep, and 3 plateau pikas were obtained and sequenced. The recombinant proteins were then prepared. Using scrapie strains (263K, 139A, ME7, and S15) as the seeds, the reactivity of the PrP proteins from sheep (rSheepPrP25-234) and pika (rPikaPrP23-230) were tested using real-time quaking-induced conversion (RT-QuIC). Protein misfolding cyclic amplification (PMCA) tests of the brain homogenates from domestic sheep and rabbits were performed with the seeds of strains 263K and ME7.

Results:

The PRNP genes of bovids were 771 bp long and encoded 256 amino acids (aa), showing 100% homology with the wild-type sheep prion protein (PrP) aa sequence. The PRNP gene of pika was 759 bp long and encoded 252 amino acids, showing 92.1% homology with the aa sequence of domestic rabbits. The sheep and pika proteins revealed positive reactions in 10-5 diluted seeds. Only rPikaPrP23-230 produced positive curves in 10-7 diluted seeds. The PMCA tests failed to produce proteinase K (PK)-resistant PrP (PrPres).

Conclusion:

This is the first description of PRNP genes and PrP aa sequences of Tibetan antelope, blue sheep, and plateau pike from the Qinghai-Tibet Plateau. In the presence of rodent prions, the PrPs of sheep and pika efficiently induce fibrillation in RT-QuIC, but do not generate PrPres in PMCA. Our results indicate that pika, as one of the important links in the Qinghai-Tibet Plateau biological chain, may play an important role in the prion circulation. Pika PrP deserves further analysis for its potential application value in assays for human prion disease.
Cardiac and Gastric Interoceptive Awareness Have Distinct Neural Substrates
Authors: Haruki Yusuke , Ogawa Kenji
Published: January 2023

Interoceptive awareness, an awareness of the internal body state, guides adaptive behavior by providing ongoing information on body signals, such as heart rate and energy status. However, it is still unclear how interoceptive awareness of different body organs are represented in the human brain. Hence, we directly compared the neural activations accompanying attention to cardiac (related to heartbeat) and gastric (related to stomach) sensations, which generate cardiac and gastric interoceptive awareness, in the same population (healthy humans,N = 31). Participants were asked to direct their attention toward heart and stomach sensations and become aware of them in a magnetic resonance imaging (MRI) scanner. The results indicated that the neural activations underlying gastric attention encompassed larger brain regions, including the occipitotemporal visual cortices, bilateral primary motor cortices, primary somatosensory cortex, left orbitofrontal cortex, and hippocampal regions. Cardiac attention, however, selectively activated the right anterior insula extending to the frontal operculum compared with gastric attention. Moreover, our detailed analyses focusing on the insula, the most relevant region for interoceptive awareness, revealed that the left dorsal middle insula encoded cardiac and gastric attention via different activation patterns, but the posterior insula did not. Our results demonstrate that cardiac and gastric attention evoke different brain activation patterns; in particular, the selective activation may reflect differences in the functional roles of cardiac and gastric interoceptive awareness.
AAV Vector Mediated Delivery of NG2 Function Neutralizing Antibody and Neurotrophin NT-3 Improves Synaptic Transmission, Locomotion, and Urinary Tract Function after Spinal Cord Contusion Injury in Adult Rats
Authors: Petrosyan Hayk A. , Alessi Valentina , Lasek Kristin , Gumudavelli Sricharan , Muffaletto Robert , Liang Li , Collins William F. , Levine Joel , Arvanian Victor L.
Published: January 2023

NG2 is a structurally unique transmembrane chondroitin sulfate proteoglycan (CSPG). Its role in damaged spinal cord is dual. NG2 is considered one of key inhibitory factors restricting axonal growth following spinal injury. Additionally, we have recently detected its novel function as a blocker of axonal conduction. Some studies, however, indicate the importance of NG2 presence in the formation of synaptic contacts. We hypothesized that the optimal treatment would be neutralization of inhibitory functions of NG2 without its physical removal. Acute intraspinal injections of anti-NG2 monoclonal antibodies reportedly prevented an acute block of axonal conduction by exogenous NG2. For prolonged delivery of NG2 function neutralizing antibody, we have developed a novel gene therapy: adeno-associated vector (AAV) construct expressing recombinant single-chain variable fragment anti-NG2 antibody (AAV-NG2Ab). We examined effects of AAV-NG2Ab alone or in combination with neurotrophin NT-3 in adult female rats with thoracic T10 contusion injuries. A battery of behavioral tests was used to evaluate locomotor function.In vivosingle-cell electrophysiology was used to evaluate synaptic transmission. Lower urinary tract function was assessed during the survival period using metabolic chambers. Terminal cystometry, with acquisition of external urethral sphincter activity and bladder pressure, was used to evaluate bladder function. Both the AAV-NG2Ab and AAV-NG2Ab combined with AAV-NT3 treatment groups demonstrated significant improvements in transmission, locomotion, and bladder function compared with the control (AAV-GFP) group. These functional improvements associated with improved remyelination and plasticity of 5-HT fibers. The best results were observed in the group that received combinational AAV-NG2Ab+AAV-NT3 treatment.

SIGNIFICANCE STATEMENTWe recently demonstrated beneficial, but transient, effects of neutralization of the NG2 proteoglycan using monoclonal antibodies delivered intrathecally via osmotic mini-pumps after spinal cord injury. Currently, we have developed a novel gene therapy tool for prolonged and clinically relevant delivery of a recombinant single-chain variable fragment anti-NG2 antibody: AAV-rh10 serotype expressing scFv-NG2 (AAV-NG2Ab). Here, we examined effects of AAV-NG2Ab combined with transgene delivery of Neurotrophin-3 (AAV-NT3) in adult rats with thoracic contusion injuries. The AAV-NG2Ab and AAV-NG2Ab+AAV-NT3 treatment groups demonstrated significant improvements of locomotor function and lower urinary tract function. Beneficial effects of this novel gene therapy on locomotion and bladder function associated with improved transmission to motoneurons and plasticity of axons in damaged spinal cord.
Linking Impulsivity to Activity Levels in Pre-Supplementary Motor Area during Sequential Gambling
Authors: Lohse Allan , Løkkegaard Annemette , Siebner Hartwig R. , Meder David
Published: January 2023

Impulsivity refers to the tendency to act prematurely or without forethought, and excessive impulsivity is a key problem in many neuropsychiatric disorders. Since the pre-supplementary motor area (pre-SMA) has been implicated in inhibitory control, this region may also contribute to impulsivity. Here, we examined whether functional recruitment of pre-SMA may contribute to risky choice behavior (state impulsivity) during sequential gambling and its relation to self-reported trait impulsivity. To this end, we performed task-based functional MRI (fMRI) after low-frequency (1 Hz) repetitive transcranial magnetic stimulation (rTMS) of the pre-SMA. We expected low-frequency rTMS to modulate task-related engagement of the pre-SMA and, hereby, tune the tendency to make risky choices. Twenty-four healthy volunteers (12 females; age range, 19–52 years) received real or sham-rTMS on separate days in counterbalanced order. Thereafter, participants performed a sequential gambling task with concurrently increasing stakes and risk during whole-brain fMRI. In the sham-rTMS session, self-reported trait impulsivity scaled positively with state impulsivity (riskier choice behavior) during gambling. The higher the trait impulsivity, the lower was the task-related increase in pre-SMA activity with increasingly risky choices. Following real-rTMS, low-impulsivity participants increased their preference for risky choices, while the opposite was true for high-impulsivity participants, resulting in an overall decoupling of trait impulsivity and state impulsivity during gambling. This rTMS-induced behavioral shift was mirrored in the rTMS-induced change in pre-SMA activation. These results provide converging evidence for a causal link between the level of task-related pre-SMA activity and the propensity for impulsive risk-taking behavior in the context of sequential gambling.

SIGNIFICANCE STATEMENTImpulsivity is a personal trait characterized by a tendency to act prematurely or without forethought, and excessive impulsivity is a key problem in many neuropsychiatric disorders. Here we provide evidence that the pre-supplementary motor area (pre-SMA) is causally involved in implementing general impulsive tendencies (trait impulsivity) into actual behavior (state impulsivity). Participants’ self-reported impulsivity levels (trait impulsivity) were reflected in their choice behavior (state impulsivity) when involved in a sequential gambling task. This relationship was uncoupled after perturbing the pre-SMA with repetitive transcranial stimulation (rTMS). This effect was contingent on trait impulsivity and was echoed in rTMS-induced changes in pre-SMA activity. Pre-SMA is key in translating trait impulsivity into behavior, possibly by integrating prefrontal goals with corticostriatal motor control.
FARKLI KURUTMA YÖNTEMLERİNİN BÖĞÜRTLEN KALİTE ÖZELLİKLERİ ÜZERİNE ETKİLERİ VE KURUMA KİNETİĞİ
Authors: DÜNDAR KIRIT Burcu , SAĞOL Sevdanur , AĞCAM Erdal , AKYILDIZ Asiye
Published: January 2023

Bu çalışmada, böğürtlenler mikrodalga (100 W) ve sıcak hava (70°C) kurutmaya tabi tutularak kuruma kinetiği parametreleri ve kurutulmuş ürününün bazı kalite özellikleri belirlenmiştir. Kurutma yönteminden bağımsız olarak araştırılan kurutma modelleri arasından Logaritmik modelin en yüksek belirlilik katsayılarına (R2=0.9813-0.9827) sahip olduğu tespit edilmiştir. Kurutulmuş böğürtlen örneklerinde, su aktivitesi, renk, hidroksimetilfurfural-(HMF), toplam monomerik antosiyanin-(TMA) ve siyanidin-3-glukozit analizleri gerçekleştirilmiştir. Sıcak hava ile kurutulan örneklerin renk parametrelerindeki (L*, a*, b* ve ∆E*) değişim mikrodalga ile kurutulanlara kıyasla daha yüksek orandadır. HMF (0.50-1.54 mg/ kg KM) oluşumunun ise mikrodalga ile kurutulan böğürtlenlerde daha düşük düzeyde olduğu gözlemlenmiştir. TMA içeriğinde (54.03-576.82 mg/100 g KM) mikrodalga kurutma ile meydana gelen azalmanın diğer yönteme kıyasla daha düşük olduğu belirlenmiştir. Benzer şekilde, siyanidin-3-glikozid (23.92 – 320.4 mg/100 g KM) sıcak hava kurutmada daha yüksek oranda parçalanmıştır. Elde edilen sonuçlara göre, kalite özellikleri ve kuruma hızı bakımından mikrodalga kurutmanın böğürtlen kurutmada önemli avantajlar sağladığı belirlenmiştir.
ULTRASON UYGULAMASININ YOĞURT BENZERİ FERMENTE SÜT ÜRÜNÜNDE FARKLI PROBİYOTİK LAKTOBASİL TÜRLERİNİN CANLILIĞI ÜZERİNE ETKİSİ
Authors: AKDENİZ Vildan
Published: January 2023

Bu çalışmada ultrason uygulamasının farklı probiyotik laktobasil (Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus rhamnosus GG) türlerinin canlılığına etkisini incelenmiştir. Ayrıca, ultrason uygulamasının fermentasyon süresine etkisi de saptanmıştır. Bu amaçla süte önce 15 dk süreyle 400 W ultrason uygulanmış ve kültür ilavesi sonrasında da 5 dk süreyle 100 W ultrason uygulanarak fermentasyon süresi belirlenmiş ve elde edilen yoğurt benzeri probiyotik fermente süt ürününde probiyotik bakteri sayımı gerçekleştirilmiştir. Yapılan incelemeler sonucunda ultrason uygulamasının L. acidophilus ve L. casei ile aşılanan örneklerde fermentasyon süresini kısalttığı ve L. acidophilus, L. casei ve L. rhamnosus GG probiyotik bakterilerinin canlılığını arttırdığı saptanmıştır. Bu sonuçlar, ultrason uygulamasının süt teknolojisinde gelecek vaat ettiğini ve probiyotik fermente süt ürünleri üretiminde kullanılma potansiyeli olduğunu göstermektedir.
IFN‐stimulated metabolite transporter ENT3 facilitates viral genome release
Authors: Hsieh Yu‐Ting , Tsai Tsung‐Lin , Huang Shen‐Yan , Heng Jian‐Wen , Huang Yu‐Chia , Tsai Pei‐Yuan , Tu Chia‐Chun , Chao Tai‐Ling , Tsai Ya‐Min , Chang Pei‐Ching , Lee Chien‐Kuo , Yu Guann‐Yi , Chang Sui‐Yuan , Dzhagalov Ivan L. , Hsu Chia‐Lin
Published: January 2023

An increasing amount of evidence emphasizes the role of metabolic reprogramming in immune cells to fight infections. However, little is known about the regulation of metabolite transporters that facilitate and support metabolic demands. In this study, we found that the expression of equilibrative nucleoside transporter 3 (ENT3, encoded by solute carrier family 29 member 3, Slc29a3) is part of the innate immune response, which is rapidly upregulated upon pathogen invasion. The transcription of Slc29a3 is directly regulated by type I interferon‐induced signaling, demonstrating that this metabolite transporter is an interferon‐stimulated gene (ISG). Suprisingly, we unveil that several viruses, including SARS‐CoV‐2, require ENT3 to facilitate their entry into the cytoplasm. The removal or suppression of Slc29a3 expression is sufficient to significantly decrease viral replication in vitro and in vivo. Our study reveals that ENT3 is a pro‐viral ISG co‐opted by some viruses to gain a survival advantage.
A toolbox for systematic discovery of stable and transient protein interactors in baker's yeast
Authors: Fenech Emma J , Cohen Nir , Kupervaser Meital , Gazi Zohar , Schuldiner Maya
Published: January 2023

Identification of both stable and transient interactions is essential for understanding protein function and regulation. While assessing stable interactions is more straightforward, capturing transient ones is challenging. In recent years, sophisticated tools have emerged to improve transient interactor discovery, with many harnessing the power of evolved biotin ligases for proximity labelling. However, biotinylation‐based methods have lagged behind in the model eukaryote, Saccharomyces cerevisiae, possibly due to the presence of several abundant, endogenously biotinylated proteins. In this study, we optimised robust biotin‐ligation methodologies in yeast and increased their sensitivity by creating a bespoke technique for downregulating endogenous biotinylation, which we term ABOLISH (Auxin‐induced BiOtin LIgase diminiSHing). We used the endoplasmic reticulum insertase complex (EMC) to demonstrate our approaches and uncover new substrates. To make these tools available for systematic probing of both stable and transient interactions, we generated five full‐genome collections of strains in which every yeast protein is tagged with each of the tested biotinylation machineries, some on the background of the ABOLISH system. This comprehensive toolkit enables functional interactomics of the entire yeast proteome.
Intensi Berwirausaha Terhadap Orientasi Masa Depan pada Mahasiswa UNNES dalam Masa Quarter Life Crisis
Authors: Priyambudi Yudit , Azis Abdul
Published: January 2023

Masa quarter life crisis dapat terjadi pada rentang usia 20-25 tahun, dimana individu dapat merasakan kebingungan serta kekhawatiran dirinya dimasa depan. Pada usia tersebut, terdapat mahasiswa UNNES yang sedang menempuh perkuliahan, maka dari itu mahasiswa UNNES dapat termasuk ke dalam masa quarter life crisis. Mahasiswa UNNES dalam proses pembelajarannya menerima banyak mata kuliah, diantaranya ialah pendidikan kewirausahaan yang dapat menjadi gambaran orientasi masa depannya. Penelitian ini bertujuan untuk mengetahui pengaruh intensi berwirausaha terhadap orientasi masa depan pada mahasiswa UNNES dalam masa quarter life crisis. Penelitian ini merupakan penelitian kuantitatif korelasional. Sampel berjumlah 344 mahasiswa UNNES yang berada dalam masa quarter life crisis dengan teknik convenience sampling. Metode analisis yang digunakan yaitu analisis regresi. Pengumpulan data dilakukan menggunakan skala intensi berwirausaha dengan koefisien reliabilitas sebesar 0,927 dari 41 item valid serta skala orientasi masa depan dengan koefisien reliabilitas sebesar 0,931 dari 30 item valid. Hasil penelitian menunjukkan bahwa ada pengaruh intensi berwirausaha terhadap orientasi masa depan pada mahasiswa UNNES dalam masa quarter life crisis. Hipotesis penelitian diterima karena diperoleh koefisien korelasi sebesar 0,729 antara intensi berwirusaha dan orientasi masa depan. Hasil uji regresi diperoleh koefisien determinasi (R square) sebesar 0,171 yang berarti 17,1% orientasi masa depan pada mahasiswa UNNES dipengaruhi oleh intensi berwirausaha.
Hubungan Antara Harga Diri dengan Perilaku Asertif pada Mahasiswa yang Aktif dalam Organisasi
Authors: Istiqomah Awanda Putri , Hariyadi Sugeng
Published: January 2023

Keterbukaaan seorang mahasiswa untuk menyampaikan aspirasi dan gagasan yang dimiliki membutuhkan adanya kemampuan berperilaku asertif. Harga diri memegang peran penting dalam pembentukan perilaku asertif, karena mahasiswa yang memiliki tingkat harga diri yang tinggi akan mampu untuk berperilaku asertif dengan baik. Namun pada kenyataannya yang terjadi pada mahasiswa yang aktif di dalam organsiasi di Kota Semarang berbeda. Nilai harga diri mereka berada pada kategori tinggi, namun belum mampu untuk berperilaku asertif. Penelitian ini bertujuan untuk mengetahui hubungan antara harga diri dengan perilaku asertif pada mahasiswa yang aktif dalam organisasi. penelitian ini merupakan penelitian kuantitatif korelasional dengan jumlah sampel 112 mahasiswa, dengan teknik sampling Proporsional Random Sampling, dengan nilai proropsi 50%. Skala yang digunakan dalam pengambilan data yaitu skala perilaku asertif yang terdiri dari 31 item dan skala harga diri yang terdiri dari 59 item. Koefisien reliabilitas perilaku asertif yaitu 0,718 dan harga diri 0,851. Metode analisis yang digunakan adalah Pearson Product Moment dengan menggunakan bantuan software pengolah data. Sedangkan uji validitasnya menggunakan uji validitas beda item. Hasil uji hipotesis menunjukkan p < 0,05 dan koefisien korelasi 0,649 dengan sign 0,000. Maka dapat disimpulkan bahwa “Ada Hubungan Positif antara Harga Diri dengan Perilaku Asertif” diterima.
Innovative Working Behavior Pada Karyawan Perusahaan Startup dan Aparatur Sipil Negara
Authors: Fatih Muhammad Abdirabbirasul ‘Azam Al
Published: January 2023

Saat ini menjadi pekerja yang kreatif dan inovatif sangatlah penting dalam mengimbangi perkembangan zaman. Selain itu setiap perusahaan maupun instansi memiliki bentuk kepemimpinan, budaya organisasi dan karakteristik pekerjaan yang berbeda sehingga dapat mempengaruhi innovative working behavior yang dimiliki oleh pekerjanya, Tujuan penelitian ini adalah untuk mengetahui perbedaan innovative working behavior pada karyawan perusahaan startup dan Aparatur Negeri Sipil (ASN), mengetahui gambaran innovative working behavior pada karyawan perusahaan startup di Jakarta dan mengetahui gambaran innovative working behavior pada Aparatur Sipil Negara (ASN) di Jakarta. Penelitian ini merupakan penelitian kuantitatif komparatif. Populasi penelitian ini adalah karyawan perusahaan startup dan Aparatur Negeri Sipil (ASN). Sampel dalam penelitian ini sebanyak 115 karyawan perusahaan startup dan 115 Aparatur Negeri Sipil (ASN) dengan menggunakan teknik non-probability sampling. Pengumpulan data dilakukan menggunakan skala innovative working behavior yang berisi 48 item dengan koefisien reliabilitas = 0,945. Hasil penghitungan mendapatkan hasil bahwa innovative pada kedua kelompok subyek penelitiani berada dalam kategori tinggi. Uji hipotesis dilakukan dengan teknik analisis Mann- Whitney U test memperoleh nilai Z sebesar -3.129 dengan nilai signifikansi (sig.) 0,002. Dengan demikian, hipotetis yang berbunyi terdapat perbedaan innovative working behavior antara karyawan perusahaan startup dengan Aparatur Sipil Negara (ASN) di Jakarta diterima.
Superstitious Belief Ditinjau dari Variasi Tempat Tinggal (Desa dan Kota)
Authors: Wulandari Maya , Muhammad Amri Hana
Published: January 2023

Superstitious belief sering dijumpai bahkan dimiliki oleh individu. Terdapat beberapa faktor yang dapat mempengaruhi salah satunya adalah lokasi tempat tinggal. Berdasarkan intensitas dan keaktifan masyarakatnya dalam menjaga budaya, tradisi dan kepercayaannya, lokasi tempat tinggal terbagi menjadi dua variasi yaitu lokasi tempat tinggal subsistem perkotaan dan subsistem non-perkotaan (desa). Penelitian ini bertujuan untuk mengetahui perbedaan superstitious belief pada masyarakat variasi tempat tinggal desa dengan variasi tempat tinggal kota, gambaran superstitious belief masyarakat desa dan masyarakat kota. Penelitian menggunakan metode kuantitatif komparasi dengan teknik sampling yaitu teknik quota sampling. Karakteristik subjek yaitu masyarakat suku Jawa, usia 18 s-d 60 tahun, dan durasi tinggal minimal 2 tahun di tempat tinggal domisili. Didapatkan sampel sejumlah 401 dengan 258 subjek kelompok variasi tempat tinggal desa dan 143 subjek kelompok variasi tempat tinggal kota. Pertanyaan penelitian 1 dijawab dengan uji hipotesisi dan pertanyaan 2 dan 3 dijawab dengan uji deskriptif. Hasil uji hipotesis menunjukan signifikansi (p) sebesar 0,001 dan mean difference sebesar 41,48, yang berarti terdapat perbedaan superstitious belief ditinjau dari variasi tempat tinggal (desa dan kota. Hasil uji deskriptif variasi tempat tinggal desa dan kota, keduanya termasuk dalam kategori sedang atau interval 88,67 ≤ X < 139,33 namun dengan perbedaan pada masyarakat variasi kota cenderung rendah.
Evaluation of Popular Recreation Places in Konya in Terms of Safety and Maintenance Criteria: In The Case of Kalehan Ecdat and Japon Parks
Authors: Güngör Sertaç
Published: January 2023

Both the negative impact of the covid pandemic that we have experienced in the last 2 years, and the understanding of having green areas with higher standards have come to the fore more today. While the longing for green spaces has increased in individuals who are closed in their homes in the pandemic environment, the necessity of these green spaces to be more well-maintained and safer has emerged, considering the pandemic conditions. The aim of this research is to determine the status of the facilities and the park in general in Kalehan-Ecdat and Japon Parks, two of the most popular urban parks in Konya, and to compare them with each other in terms of maintenance and security. These two popular parks constitute the main material of the research. A total of 256 visitors using the parks were surveyed on a voluntary basis, paying attention to the pandemic hygiene conditions, face-to-face and online using Google surveys. Kalehan-Ecdat and Japon parks were evaluated by the park users in terms of the well-maintained and safety of the facilities they contain and the park in general, in order to determine their current status and to compare them with each other. While 60% of the users found both parks well-maintained, 4% found them unmaintained. When the security conditions of the parks were evaluated, Kalehan-Ecdat park, which has straight access roads and patrols in the park, was found to be 70% safe in terms of circulation, while the Japon park, which had deserted areas in places due to its lively design, was found to be 50% safe.
Examination of Shopping Malls on The Usage Preferences of Physical Disabilities: On-Site Evaluation From Konya/Turkey
Authors: Güngör Sertaç , İyit Nesihan
Published: January 2023

With this research, the suitability of the existing large shopping malls in the city of Konya for the use of physically disabled individuals, the problems they may experience in their use and their expectations were evaluated. For this purpose, a survey was conducted in 4 large shopping malls, private or public rehabilitation malls for the disabled, and the Konya Branch of the Turkish Association for the Disabled. The research was carried out in 2018-2019. A survey was conducted with disabled people who volunteered on different days, weekdays, and weekends. Even though it is difficult to access, the shopping mall where they can be most comfortable as a disabled person has been “Novada”. While "Novada AVM" stands out due to the ease of use of the sanitary facilities, the presence of landmarks for the visually impaired, the presence of a battery-chair charging station and the availability of wheelchairs, "Kent plaza", where its staff receives "sign language" training for the speech-impaired, is one of the preferred shopping malls. has been. In this research, "Kulesite" shopping mall has been the most preferred shopping mall due to the ease of transportation by "tramway".
Comparison of Plant Detection Performance of CNN-based Single-stage and Two-stage Models for Precision Agriculture
Authors: OZCAN RECAI , Tutuncu Kemal , KARACA MURAT
Published: January 2023

The fact that arable land is not increasing in proportion to the ever-increasing population will increase the need for food in the coming years. For this reason, it is necessary to increase the yield of crops to make optimum use of arable land. One of the most important reasons for the decrease in yield and quality of crops is weeds. Herbicides are generally preferred for weed management. Due to deficiencies in herbicide application methods, only 0.015-6% of herbicides reach their target. The use of herbicides, which is an important part of the agricultural system, is an issue that needs to be emphasized, considering the risk of residue and environmental damage. In parallel with the rapid development of electronic and computer technologies, artificial intelligence applications have had the opportunity to develop. In this context, the use of artificial intelligence for plant detection in the subsystems of herbicide application machines will contribute to the development of precision agriculture techniques. In this study, the plant detection performances of single-stage and two-stage Convolutional Neural Network (CNN)-based deep learning (DL) models are evaluated. In this context, a dataset was created by taking images of Zea mays, Rhaponticum repens (L.) Hidalgo, and Chenopodium album L. plants in agricultural lands in Konya. With this dataset, the training of the models was carried out by the transfer learning method. The evaluation metrics of the trained models were calculated using the error matrix. In addition, training time and prediction time were used as quantitative metrics in the evaluation of the models. The plant detection performance, training time, and prediction time of the models were 85%, 8 h, 1.21 s for SSD MobileNet v2 and 99%, 22 h, 2.32 s for Faster R-CNN Inception v2, respectively. According to these results, Faster R-CNN Inception v2 is outperform in terms of accuracy. However, in cases where training time and prediction time are important, the SSD MobileNet v2 model can be trained with more data to increase its accuracy.
Effects of Climate Changes on Rose Fungal and Bacterial Diseases in Lands-cape Areas of Konya Province, Türkiye
Authors: ÖZTÜRK Aysun , BASTAS Kubilay Kurtulus
Published: January 2023

Rose, a plant belonging to the family Rosaceae, is one of the most popular and versatile flowering shrubs in urban landscape areas. In recent years, rose bacterial diseases are getting to increase and they cause significant economic losses. The severity and distribution of these problems change every year according to the changing climatic factors, growing areas, the type of roses and the level of being affected by biotic and abiotic factors. In this study, on the 106 rose cultivars located in landscape areas of Konya province was determined different diseases symptoms at various levels by carried out survey studies in 1998-2022 years. As a result of the diagnosis of obtaining bacterial and fungal isolates, bacterial blights (Pseudomonas syringae pv. syringae and P. s. pv. morsprunorum), Xanthomonas hortorum, fire blight (Erwinia amylovora) and crown gal (Rhizobium radiobacter), downy mildew (Peronospara sparsa), rust (Phragmodium mucronatum), black spot (Diplocarpon rosae), powdery mildew (Sphaerotheca pannosa var. rosae), gray mold (Botrytis cinerea) were determined as the pathogen on the rose cultivars. It is thought that the findings obtained from the study will contribute to the future development of rose cultivation by revealing the different pathogens and disease levels in roses as a result of the changing climatical conditions.
Proso millet (Panicum miliaceum L) Cultivation Form and Potentiality under Konya Conditions, Türkiye
Authors: Acar Ramazan , ASLANDAĞ Yakup , Osman Ibrahim Musa
Published: January 2023

İncreasing climate change effect with decreaing and limation of food sources, alternative and well adopted pathway should used. Proso millet is one of human food alternative, its dry enviroment crops. Panicum miliaceum is used in human and animal nutrition. Recently it has been grown in the Konya region in an irrigated schemes for its grain. Proso millet grains have vital economic and nutrition potential. Previously were used to feed pet birds and poultary. Under Konya conditions, the farmer obtained about 150-200 kg/da grain yield. Crop seeds are harvested in 90-100 days, using a combined harvester. The crop possesses a vital capacity of being a promising crop that might contribute to human food security
Valorar o demoler el patrimonio moderno: el caso del edificio de la ESSO Colombiana S.A. en la ciudad de Bogotá, Colombia
Authors: Largacha Martínez María Olga
Published: January 2023

Se habla mucho de la urgencia de salvaguardar el patrimonio inmueble de la ciudad de Bogotá, a la vez que se radican planes parciales de renovación para ser avalados por la Secretaría Distrital de Planeación, que en ocasiones entran en contradicción con esta urgencia que busca la respectiva declaratoria como Bien de Interés Cultural (BIC) de dichas edificaciones. Es el caso del Plan Parcial de Renovación Urbana CAR- Universidad Libre, que aparece como con Viabilidad en la página de la Secretaría Distrital de Planeación el cual contiene, dentro de su polígono de intervención, dos edificios imprescindibles para la memoria arquitectónica de Bogotá. Uno de ellos el de Pan American (Edificio Teusacá), el cual afortunadamente ya tiene declaratoria BIC. El otro, el Edificio de la Esso Colombiana, aún no cuenta con esta. Este texto refiere y particulariza este edificio a partir de la revisión de su planimetría y características arquitectónicas en ejercicio investigativo realizado por la autora acerca de Ignacio Martínez Cárdenas y su ejercicio profesional. La investigación parte del siguiente interrogante: ¿Cuál es el argumento para evadir la responsabilidad que tenemos de proteger como conocedores de lo que significa, implica y representa este edificio, en esa localización y con esa clara declaración formal, estética, conceptual y material, manifiesta aún hoy en esta particular edificación?
ICTS and computational thinking to foster language skills: a proposal based on Scratch
Authors: Tinedo Rodríguez Antonio Jesús
Published: January 2023

Language learning evolves in parallel with technological advances. The use of ICT has proven to be very useful in language teaching and new studies underline the importance of computational thinking. The objective of this article is to design a pedagogical proposal in which parents, teachers and students are expected to be involved. This proposal is supposed to foster the use ICT and computational thinking as a language immersion environment outside the classroom. To do so, the software Scratch is at the very core of the proposal. In conclusion, the expected results of this design are an improvement in linguistic competence, technological skills and the family-school relationship.
Wartime economics: challenges and rapid economic policy
Authors:   Kyiv National University of Economics named after Vadym Hetman, Moskalenko Oleksandra ,   Vienna University of Economics and Business
Published: January 2023

The article provides an overview and reflection of quick decisions on economic policies in Ukraine under wartime conditions. The author defines the theoretical and historical background of wartime economy, as well as its content and tasks. Presented various historical examples of the consequences of wars on the economy and its post-war recovery. It is found that in addition to the fact that wars have negative consequences for physical and human capital, they may also speed up economic growth after the war due to the change in economic environment. The author provides a brief analysis of economic policies in the wartime economy of Ukraine, challenges and quick decisions in fiscal and monetary policy, human capital preservation policy, energy policy and regulatory policy of coordinating processes during the war. There is an analysis of proposed changes to the tax legislation, and monetary decisions during the war and other steps taken by the government and the NBU to support the economy in the conditions of war shocks. The acuteness of the demographic challenge for Ukraine due to the loss of human capital and forced emigration is demonstrated. Presented various macroeconomic data on GDP decline during the war, losses, inflation, international aid by donors, which contributes to critical review of the quick economic policy decisions taken. The author undertakes a theoretical generalization and outlines the key challenges for the post-war reconstruction of Ukraine and quick decisions, which topics are among the subjects of discussions on international platforms, as well as objects for analysis in search of ways and means of international partnership support. The differences between rapid and adequate economic policy are shown.