bluuebunny/crossref_metadata_2025 huggingface dataset:
An early HMGB1 rise 12 hours before creatinine predicts acute kidney injury and multiple organ failure in a smoke inhalation and burn swine model
Authors: Yang Zhangsheng , Cancio Tomas S. , Willis Robert P. , Young Matthew D. , Kneifel Dustin M. , Salinas Jose , Meyer Andrew D.
Published: October 2024

Background

Acute kidney injury (AKI) and multiple organ failure (MOF) are leading causes of mortality in trauma injuries. Early diagnosis of AKI and MOF is vital to improve outcomes, but current diagnostic criteria rely on laboratory markers that are delayed or unreliable. In this study, we investigated whether damage associated molecular patterns such as high-mobility group box 1 (HMGB1), syndecan-1 (SDC-1) and C3a correlate with the development of trauma-induced AKI and MOF.

Methods

Thirty-nine swine underwent smoke inhalation and severe burns, then received critical care for 72 hours or until death. AKI was defined by the KDIGO (Kidney Disease: Improving Global Outcomes) criteria, which labels AKI when a 1.5-fold increase in blood creatinine levels from baseline or a urine output < 0.5 mL/kg/h for 6 hours or more occurs. MOF was defined by the presence of both AKI and acute respiratory distress syndrome (PaO2/FiO2<300 for 4 hours).

Results

Eight of 39 pigs developed AKI and seven of those developed MOF. Pathological analysis revealed that polytrauma induces significantly higher kidney injury scores compared to sham controls. The average time from injury to KDIGO AKI was 24 hours (interquartile range: 22.50-32.25). Twelve hours after injury, HMGB1 levels were significantly increased in animals that went on to develop AKI compared to those that did not (73.07 ± 18.66 ng/mL vs. 31.64 ± 4.15 ng/mL, p<0.01), as well as in animals that developed MOF compared to those that did not (81.52±19.68 ng/mL vs. 31.19 ± 3.972 ng/mL, p<0.05). SDC-1 and C3a levels were not significantly different at any time point between groups. ROC analysis revealed that HMGB1 levels at 12 hours post-injury were predictive of both AKI and MOF development (AKI: AUROC=0.81, cut-off value=36.41 ng/mL; MOF: AUROC=0.89, cut-off value=36.41 ng/mL). Spearman’s correlation revealed that HMGB1 levels at 12 hours correlated with multiple parameters of AKI, including blood urea nitrogen, blood creatinine, and blood myoglobin.

Conclusion

Twelve-hour post-injury HMGB1 levels predict AKI and MOF in a smoke inhalation and burn swine model. Further research is needed to validate this result in other polytrauma models and in critical combat causalities.
Arterial blood gases in SCUBA divers at depth
Authors: Paganini Matteo , Zucchi Lorenzo , Giacon Tommaso Antonio , Martani Luca , Mrakic-Sposta Simona , Garetto Giacomo , McKnight J. Chris , Camporesi Enrico M. , Moon Richard E. , Bosco Gerardo
Published: October 2024

Background

Current diving physiology postulates that SCUBA divers’ arterial blood gas (ABG) levels vary proportionally to environmental pressure, but, to date, ABGs have only been obtained during simulated dives. Also, recent evidence supports the use of the arterial/alveolar (a:A) partial pressures of oxygen (PO2) ratio to predict the arterial PO2 (PaO2) under hyperbaric conditions from measurements obtained at 1 atmosphere absolute (ATA). This work summarizes ABGs obtained in SCUBA divers in real underwater conditions and aims to validate the a:A ratio in predicting PaO2 in this subset of individuals at depth.

Methods

The study was approved by the local ethics committee. After cannulating the radial artery of the non-dominant limb, ABGs were sampled at the surface before the dive (A), at depth (15 meters of freshwater (mfw) or 42 mfw) before (B) and after (C) pedaling on a submersed bicycle for 10 minutes, and back at surface (D). After calculating the surface alveolar PO2 for each subject, the a:A ratio was obtained and used to predict PaO2 at depth. A linear regression between measured and predicted PaO2 was reported, along with the goodness-of-fit F test.

Results

Six subjects performed the dive at 15 mfw, and four others at 42 mfw. The PaO2 proportionally increased at both depths, remaining stable before and after pedaling. The a:A calculated from the baseline ABG obtained at rest, out of the water, adequately predicted the PaO2 at depth (R2 = 0.97, p<0.001), better at 15 mfw but losing accuracy at 42 mfw.

Conclusions

The ABGs confirmed the proportional rise of PaO2 in SCUBA divers underwater. The a:A ratio could be used to predict the magnitude of PaO2 rise at depth to limit exposure to hyperoxia, especially in repetitive recreational dives and professional divers.
Bibliometric and visualized analysis of peripartum respiratory complications from 2004 to 2023
Authors: Ye Fan , Wang Teng , Chen Yijiao , Li Fan , Gu Xinyu , Xiao Jie
Published: October 2024

Background

Peripartum respiratory complications are a major reason for pregnant women’s admission to the ICU, even endangers the maternal life. This bibliometric analysis was designed to elucidate the spectrum of diseases and risk factors for various respiratory complications during the peripartum period, as well as the future research directions in this field.

Methods

Relevant publications were downloaded from the Web of Science Core Collection on November 1, 2023. CiteSpace was utilized for conducting the scientometric study.

Results

A total of 2,331 articles and reviews on respiratory complications during pregnancy published between 2004 and 2023 were retrieved, comprising 87,244 co-cited references, originating from 568 institutions across 104 countries/regions. The United States emerged as the leading country in this domain, with Harvard University standing out as the most actively engaged institution. Bibliometric analysis reveals that the current research hotspots include “COVID-19 pandemic,” “venous thromboembolism,” “respiratory distress syndrome” and “cardiovascular diseases.” Meanwhile, “venous thromboembolism,” cytokine storm” and supportive management such as “extracorporeal membrane oxygenation” might represent potential future research directions.

Conclusion

Over the past two decades, research on respiratory system complications in pregnancy has continually evolved. This study contributes to enabling researchers in the related field to understand future research hotspots and trends, providing information on potential collaborators, institutions, countries, and citation references.
A case report: cccDNA and pgRNA remain positive in liver tissue in a chronic hepatitis B patient with functional cure
Authors: Xu Wenxiong , Luo Qiumin , Zhang Yeqiong , Xie Chan , Peng Liang
Published: October 2024

Hepatitis B surface antigen (HBsAg) seroclearance is recommended as the ideal endpoint for nucleos(t)ide analog (NA) treatments. Functional cure of chronic hepatitis B (CHB) is defined as having undetectable serum hepatitis B virus (HBV) deoxyribonucleic acid and serum HBsAg. We report a functional cure case of CHB with a family history of hepatocellular carcinoma (HCC) after long-term NA therapy. Despite achieving functional cure for over 7 years, both HBV covalently closed circular deoxyribonucleic acid (cccDNA) and pregenomic ribonucleic acid (pgRNA) remain positive in the liver tissue of the patient, indicating that a sterilizing cure has not been achieved. This case highlights the importance of active surveillance of HBV cccDNA and pgRNA for sterilizing the cure and risk of HCC.
The influence of depth of sedation on motor evoked potentials monitoring in youth from 4 to 23 years old: preliminary data from a prospective observational study
Authors: Hudec Jan , Kosinová Martina , Prokopová Tereza , Zelinková Hana , Hudáček Kamil , Repko Martin , Gál Roman , Štourač Petr
Published: October 2024

Introduction

The influence of various levels of sedation depth on motor evoked potentials (MEP) reproducibility in youth is still unclear because of a lack of data. We tested the hypothesis that a deeper level of total intravenous anesthesia (TIVA) [bispectral index (BIS) 40 ± 5 compared to 60 ± 5] can affect surgeon-directed MEP and their interpretation in youths.

Methods

All patients received TIVA combined with propofol and remifentanil. TIVA was initially maintained at a BIS level of 60 ± 5. The sedation anesthesia was deepened to BIS level 40 ± 5 before the skin incision. MEP were recorded and interpreted at both BIS levels. The primary endpoint was to evaluate the effect of the depth of sedation on the MEP reproducibility directed and interpreted by the surgical team in each patient separately. The secondary endpoint was to compare the relativized MEP parameters (amplitude and latency) in percentage at various levels of sedation in each patient separately. We planned to enroll 150 patients. Due to the COVID-19 pandemic, we decided to analyze the results of the first 50 patients.

Results

The surgical team successfully recorded and interpreted MEP in all 50 enrolled patients in both levels of sedation depth without any clinical doubts. The MEP parameters at BIS level 40 ± 5, proportionally compared with the baseline, were latency 104% (97–110%) and the MEP amplitudes 84.5% (51–109%).

Conclusion

Preliminary data predict that deeper sedation (BIS 40 ± 5) does not affect the surgical team’s interpretation of MEP in youth patients. These results support that surgeon-directed MEP may be an alternative when neurophysiologists are unavailable.
Monitoring of Bacillus spore-forming dynamics through flow cytometry
Authors: Chen Zhili , Lu Yuanyuan , Cui Jiazhen , Feng Yuzhong , Dong Haolong , Huang Xuan , Zhu Chen , Xiong Xianghua , Chen Huipeng , Wang Qingyang , Liu Gang
Published: October 2024

The plate counting method is a traditional and widely accepted technique for live cell counting, often employed for Bacillus enumeration and spore forming rate calculations. However, this method requires at least 12 h to generate results, making it unsuitable for real-time monitoring of bacterial growth status and spore transformation rate. Bacillus thuringiensis crystals, produced during sporulation, are widely used as microbial pesticides, with high demand for industrial scale production. Variations in cultivation conditions and harvest timing during large-scale pore production of Bacillus thuringiensis significantly affect spore forming rate, impacting crystallization yield. Nevertheless, there is a lack of real-time monitoring methods for spore conversion rate. Flow cytometry (FCM), a well-established technique for single-cell analysis in eukaryotic cells, has been successfully applied in bacterial detection in environmental and food samples. In this study, we introduced a rapid flow cytometry-based method for determining spore forming rate of Bacillus thuringiensis, with two nucleic acid dyes, SYTO24 and LDS751. The method enables dynamic monitoring of spore, vegetative cell, and viable but non-culturable/dead cell proportions during the whole cultivation process, and spore forming rate could be gained within 30 min. Data of spore forming rate by FCM method is consistent with that by plate counting method, offering a faster and more efficient approach for assessing sporulation status in industrial Bacillus thuringiensis microbial pesticide production.
Application of an in vitro neuroinflammation model to evaluate the efficacy of magnesium-lithium alloys
Authors: Bhat Krathika , Helmholz Heike , Willumeit-Römer Regine
Published: October 2024

Mg-Li alloys can be promising candidates as bioresorbable Li-releasing implants for bipolar disorder and other neurodegenerative disorders. In order to compare the therapeutic efficacy of conventional Li salts and Li delivered through Mg-Li alloy extracts, we tested an in vitro model based on the neuroinflammation hypothesis of mood disorders (peripheral inflammation inducing neuroinflammation) wherein, a coculture of microglia and astrocytes was treated with conditioned medium from pro-inflammatory macrophages. Two alloys, Mg-1.6Li and Mg-9.5Li, were tested in the form of material extracts and well-known outcomes of Li treatment such as GSK3β phosphorylation (indirect flow cytometry) and influence on inflammation-related gene expression (qPCR) were compared against Li salts. This is the first study demonstrating that Li can increase the phosphorylation of GSK3β in glial cells in the presence of excess Mg. Furthermore, Mg-Li alloys were more effective than Li salts in downregulating IL6 and upregulating the neurotrophin GDNF. Mg had no antagonistic effects toward Li-driven downregulation of astrogliosis markers. Overall, the results provide evidence to support further studies employing Mg-Li alloys for neurological applications.
Region-independent active CNS net uptake of marketed H+/OC antiporter system substrates
Authors: Bällgren Frida , Hu Yang , Li Shannuo , van de Beek Lara , Hammarlund-Udenaes Margareta , Loryan Irena
Published: October 2024

The pyrilamine-sensitive proton-coupled organic cation (H+/OC) antiporter system facilitates the active net uptake of several marketed organic cationic drugs across the blood-brain barrier (BBB). This rare phenomenon has garnered interest in the H+/OC antiporter system as a potential target for CNS drug delivery. However, analysis of pharmacovigilance data has uncovered a significant association between substrates of the H+/OC antiporter and neurotoxicity, particularly drug-induced seizures (DIS) and mood- and cognitive-related adverse events (MCAEs). This preclinical study aimed to elucidate the CNS regional disposition of H+/OC antiporter substrates at therapeutically relevant plasma concentrations to uncover potential pharmacokinetic mechanisms underlying DIS and MCAEs. Here, we investigated the neuropharmacokinetics of pyrilamine, diphenhydramine, bupropion, tramadol, oxycodone, and memantine. Using the Combinatory Mapping Approach for Regions of Interest (CMA-ROI), we characterized the transport of unbound drugs across the BBB in specific CNS regions, as well as the blood-spinal cord barrier (BSCB) and the blood-cerebrospinal fluid barrier (BCSFB). Our findings demonstrated active net uptake across the BBB and BSCB, with unbound ROI-to-plasma concentration ratio, Kp,uu,ROI, values consistently exceeding unity in all assessed regions. Despite minor regional differences, no significant distinctions were found when comparing the whole brain to investigated regions of interest, indicating region-independent active transport. Furthermore, we observed intracellular accumulation via lysosomal trapping for all studied drugs. These results provide new insights into the CNS regional neuropharmacokinetics of these drugs, suggesting that while the brain uptake is region-independent, the active transport mechanism enables high extracellular and intracellular drug concentrations, potentially contributing to neurotoxicity. This finding emphasizes the necessity of thorough neuropharmacokinetic evaluation and neurotoxicity profiling in the development of drugs that utilize this transport pathway.
EEG microstate as a biomarker of post-stroke depression with acupuncture treatment
Authors: Wei Conghui , Yang Qu , Chen Jinling , Rao Xiuqin , Li Qingsong , Luo Jun
Published: October 2024

Background

Post-stroke depression (PSD) is a prevalent psychiatric complication among stroke survivors. The PSD researches focus on pathogenesis, new treatment methods and efficacy prediction. This study explored the electroencephalography (EEG) microstates in PSD and assessed their changes after acupuncture treatment, aiming to find the biological characteristics and the predictors of treatment efficacy of PSD.

Methods

A 64-channel resting EEG data was collected from 70 PSD patients (PSD group) and 40 healthy controls (HC group) to explore the neuro-electrophysiological mechanism of PSD. The PSD patients received 6 weeks of acupuncture treatment. EEG data was collected from 60 PSD patients after acupuncture treatment (MA group) to verify whether acupuncture had a modulating effect on abnormal EEG microstates. Finally, the MA group was divided into two groups: the remission prediction group (RP group) and the non-remission prediction group (NRP group) according to the 24-Item Hamilton Depression Scale (HAMD-24) reduction rate. A prediction model for acupuncture treatment was established by baseline EEG microstates.

Results

The duration of microstate D along with the occurrence and contribution of microstate C were reduced in PSD patients. Acupuncture treatment partially normalized abnormal EEG microstates in PSD patients. Baseline EEG microstates predicted the efficacy of acupuncture treatment with an area under the curve (AUC) of 0.964.

Conclusion

This study provides a novel viewpoint on the neurophysiological mechanisms of PSD and emphasizes the potential of EEG microstates as a functional biomarker. Additionally, we anticipated the therapeutic outcomes of acupuncture by analyzing the baseline microstates, which holds significant practical implication for the PSD treatment.
Th17/Treg cell balance in patients with papillary thyroid carcinoma: a new potential biomarker and therapeutic target
Authors: Huo Meng-Han , Adeerjiang Yilinuer , Abulitipu Ayiguzhali , Khan Umair , Li Xin-Xi , Zhang Lei , Tian Ye , Jiang Sheng , Xu Can-Can , Chao Xian-Zhen , Yang Ye-Fan , Zhang Jin-Xia , Du Guo-Li
Published: October 2024

Papillary thyroid carcinoma (PTC) is the most common subtype of thyroid carcinoma. The most effective treatment for PTC is surgical resection, and patients who undergo surgery have good survival outcomes, but some patients have distant metastasis or even multiorgan metastases at the time of initial diagnosis. Distant metastasis is associated with poorer prognosis and a higher mortality rate. Helper T lymphocyte 17 (Th17) cells and regulatory T lymphocytes (Tregs) play different roles in PTC, and the Th17/Treg balance is closely related to the progression of PTC. Th17 cells play anticancer roles, whereas Tregs play cancer-promoting roles. A Th17/Treg imbalance promotes tumor progression and accelerates invasive behaviors such as tumor metastasis. Th17/Treg homeostasis can be regulated by the TGF‐β/IL‐2 and IL‐6 cytokine axes. Immune checkpoint inhibitors contribute to Treg/Th17 cell homeostasis. For PTC, monoclonal antibodies against CTLA-4, PD-1 and PD-L1 inhibit the activation of Tregs, reversing the Th17/Treg cell imbalance and providing a new option for the prevention and treatment of PTC. This article reviews the role of Tregs and Th17 cells in PTC and their potential targets, aiming to provide better treatment options for PTC.
Treatment of brain metastases from non-small cell lung cancer: preclinical, clinical, and translational research
Authors: Sampat Parth J. , Cortese Alyssa , Goodman Alexandra , Ghelani Ghanshyam H. , Mix Michael D. , Graziano Stephen , Basnet Alina
Published: October 2024

Lung cancer is the second most common type of cancer and is the leading cause of cancer-related deaths in the United States. Approximately 10-40% of patients with solid tumors develop brain metastases, with non-small cell lung cancer accounting for approximately 50% of all cases of patients with brain metastases. Many management options are available which can include surgery, radiation, and systemic therapy. A variety of factors go into the selection of management of brain metastases. In this review, we will focus on the treatment strategies and optimizing the management of brain metastases in patients with non-small cell lung cancer.
Effectiveness of a school-based high-intensity interval training intervention in adolescents: study protocol of the PRO-HIIT cluster randomised controlled trial
Authors: Liu Yong , Barker Alan R. , Adlam Anna-Lynne R. , Li Minghui , Duncombe Stephanie L. , Agbaje Andrew O. , Gu Yaodong , Zhou Huiyu , Williams Craig A.
Published: October 2024

Introduction

High-intensity interval training (HIIT) is an effective strategy for improving a variety of health and fitness outcomes within school settings. Incorporating HIIT into existing physical activity opportunities appears practically feasible, yet the process evaluation and effectiveness of this strategy needs to be further evaluated. Therefore, a PRO-HIIT intervention will be conducted to evaluate the effectiveness of a 12-week school-based HIIT intervention on cardiorespiratory fitness, physical activity, body composition, muscular strength, bone health, cognitive function, wellbeing and academic performance among 12–13-year-olds.

Methods and analysis

Eight classes of year 7 students (12–13-year-olds) from a secondary school in Ningbo, China, will be recruited and randomly allocated into an intervention or control group. While the control group maintains their usual activities, a 6 to 10 min HIIT session will be embedded in the physical education or physical activity lessons five days a week for 12 weeks for the intervention group. Training workshops will be conducted for participants, teachers, and research staff for facilitating the intervention. Outcome data will be collected at three time points: pre- and post-intervention, and two months (summer holiday) upon completion of the intervention. Linear mixed models will be used to analyse the impact of groups (intervention and control), timepoints (pre-, post- and two-month after intervention) and group by time interactions. The implementation process of the intervention will be evaluated using a process evaluation framework.

Ethics and dissemination

Ethics approval is obtained from the Ningbo University Ethics Committee (TY2024002). Results from PRO-HIIT study will be disseminated via peer-reviewed journals, scientific conferences as well as local education system. The study protocol has been retrospectively registered on ClinicalTrials.gov Protocol Registration and Results System (NCT06374732), https://clinicaltrials.gov/study/NCT06374732.
Precision pharmacotherapy of atomoxetine in children with ADHD: how to ensure the right dose for the right person?
Authors: Guo Hong-Li , Huang Jian , Wang Jie , Fan Lin , Li Yue , Wu Dan-Dan , Liu Qian-Qi , Chen Feng
Published: October 2024

Non-stimulant atomoxetine is recognized in various current clinical guidelines as an important alternative to stimulants for the pharmacological treatment of attention deficit/hyperactivity disorder (ADHD) in children. While its efficacy and tolerability for core symptoms are established, there is considerable inter-individual variability in response and exposure, highlighting the need for personalized dosing. In this review, we evaluated existing studies and summarized comprehensive evidence supporting the clinical implementation of therapeutic drug monitoring (TDM) and personalized dosing of atomoxetine, organized around a series of logically structured questions. Although there are notable gaps in achieving personalized dosing across multiple critical elements, the available evidence is helpful to endorse personalized dose adjustments based on TDM and CYP2D6 genotyping “whenever possible.” We advocate for ongoing improvement and enhancement in clinical practice. Future advancements will rely on a deeper understanding of ADHD, facilitating more precise diagnoses and personalized treatment strategies.
Fibrillin gene family and its role in plant growth, development, and abiotic stress
Authors: El-Sappah Ahmed H. , Li Jia , Yan Kuan , Zhu ChaoYang , Huang Qiulan , Zhu Yumin , Chen Yu , El-Tarabily Khaled A. , AbuQamar Synan F.
Published: October 2024

Fibrillins (FBNs), highly conserved plastid lipid-associated proteins (PAPs), play a crucial role in plant physiology. These proteins, encoded by nuclear genes, are prevalent in the plastoglobules (PGs) of chloroplasts. FBNs are indispensable for maintaining plastid stability, promoting plant growth and development, and enhancing stress responses. The conserved PAP domain of FBNs was found across a wide range of photosynthetic organisms, from plants and cyanobacteria. FBN families are classified into 12 distinct groups/clades, with the 12th group uniquely present in algal–fungal symbiosis. This mini review delves into the structural attributes, phylogenetic classification, genomic features, protein–protein interactions, and functional roles of FBNs in plants, with a special focus on their effectiveness in mitigating abiotic stresses, particularly drought stress.
First De Novo genome assembly and characterization of Gaultheria prostrata
Authors: Lin Yan-Jun , Ding Xiao-Ya , Huang Yi-Wei , Lu Lu
Published: October 2024

Gaultheria Kalm ex L. (Ericaceae), a type of evergreen shrub, known as a natural source of methyl salicylate, possesses rich germplasm resources, strong habitat adaptability, significant ornamental value, and noteworthy pharmacological activities. However, due to the paucity of whole genomic information, genetically deep research in these areas remains limited. Consequently, we intend to obtain genome data through high-throughput sequencing, gene annotation, flow cytometry, transcription factors prediction and genetic marker analysis for a representative species of this genus, with Gaultheria prostrata selected for our study. In this study, we preliminarily obtained the genome of G. prostrata through next-generation sequencing methods. Utilizing 47.94 Gb of high-quality sequence data (108.95× coverage), assembled into 114,436 scaffolds, with an N50 length of 33,667 bp. The genome size assembled by SOAPdenovo, approximately 417 Mb, corresponded closely to predictions by flow cytometry (440 Mb) and k-mer analysis (447 Mb). The genome integrity was evaluated using BUSCO with 91%. The heterozygosity ratio was 0.159%, the GC content was 38.85%, and the repetitive regions encompassed over 34.6% of the genome. A total of 26,497 protein-coding genes have been predicted and annotated across Nr, Swissprot, GO, KEGG, and Pfam databases. Among these, 14,377 and 2,387 genes received functional annotation in Nr and Swissprot, respectively; 21,895, 24,424, and 22,330 genes were similarly annotated in GO, KEGG, and Pfam. Moreover, A total of 279,785 SSRs were identified and 345,270 primers for these SSRs were designed. Within the various nucleotide types of SSRs, AG/CT and AAG/CTT constituted the predominant dinucleotide and trinucleotide repeat types in G. prostrata. In addition, 1,395 transcription factors (TFs) from 75 TF families, 462 transcription regulators (TRs) from 33 TR families and 840 protein kinase (PKs) from 118 PK families were identified in this genome. We also performed phylogenetic analyses of G. prostrata and related species, including estimation of divergence times and expansion and contraction analyses, followed by positive selection analyses of orthologous gene pairs of G. prostrata and its close relative Vaccinium corymbosum. These results provide a reference for in-depth study of genus Gaultheria, contributing to future functional and comparative genomics analyses and providing supporting data for the development of molecular markers.
Terahertz spectroscopy study of oridonin and ponicidin in the anticancer Chinese herbal medicine Rabdosia rubescens
Authors: Gao Yang , Peng Zhuang , Yang Huiyu , Zhang Xinrui , Zhao Yuhan , Hou Zeyu , Su Bo , Li Kai , Zhang Cunlin
Published: October 2024

Rabdosia rubescens, a Chinese herbal medicine with anticancer properties, contains two active ingredients: oridonin and ponicidin. Both compounds exhibit antitumor effects by inducing tumor cell apoptosis and autophagy and inhibiting tumor cell proliferation. To further explore the differences in molecular structure and pharmacological properties between the two substances, this study employs Terahertz Time-Domain Spectroscopy (THz-TDS) to investigate the spectral characteristics of oridonin and ponicidin in the frequency range of 0.1 to 2.3 THz. The crystal structures of the two substances are simulated using Materials Studio software and Density Functional Theory (DFT), yielding their spectra and molecular vibration modes, which elucidate the mechanism underlying the peak generation. The consistency between experimental and simulation results confirms the reliability of the experimental findings. Thus, THz spectroscopy can effectively distinguish between these two substances. Finally, a THz detection is performed on Rabdosia rubescens capsules purchased from the market, revealing the presence of absorption peaks for both substances in their absorption spectra. This provides a new approach for detecting active ingredients in Chinese herbal medicines.
Comparative plastomes sheds light on phylogeny of Weigela
Authors: Wang Lei , Li Fuxing , Zhao Kexin , Yang Jie , Sun Haonan , Cui Xingyong , Dong Wenpan , Li Enze , Wang Ning
Published: October 2024

Weigela Thunb. is a genus in the family Caprifoliaceae. All species in this genus have high ornamental and medicinal value. However, the genetic divergence between species and the phylogeny within Weigela is still unclear. Therefore, we sequenced and analyzed four plastomes from four different Weigela species to reveal the genetic divergence among species of this genus, and the phylogeny within Weigela. The four plastomes from Weigela ranged from 156,909 bp to 157,739 bp in size, and presented a typical circular quadripartite structure. Each complete plastome contained a pair of inverted repeat regions (23,592~24,957 bp), a larger single-copy (LSC) region (89,922~90,229 bp), and a small single-copy (SSC) region (17,668~20,429 bp). We identified three types of repeats, corresponding to 268 forward repeats, 128 palindromic repeats, and 867 tandem repeats, for a total of 1,263 long repeats. A total of 352 SSRs were identified from the four plastomes, and most of them were concentrated in the LSC region and the noncoding regions. Mononucleotide repeat units were the most frequently detected types of repeats, of which A/T repeat units were the most abundant. Three mutational hotspots (trnH-psbA, trnR-ndhF, and trnN-ndhF) were identified as candidate barcodes for Weigela species. Weigela belongs to Diervilloideae located at an early diverging position in the Caprifoliaceae. Within Weigela, W. japonica and W. floribunda were sister with W. subsessilis and W. florida. This study revealed the plastome structure and variation of four well-known Weigela species, and found three candidate barcodes for further study of four well-known Weigela species. In addition, the phylogenetic location of Weigela within the Caprifoliaceae was identified.
The EU democracy policy in Latin America: narrative and funding
Authors: Povse Max , Chaguaceda Noriega Armando
Published: October 2024

Democracy is a central concern in the EU narrative toward Latin America; still, the allocation of funds does not follow this narrative closely, resulting in a chronic lack of support for local CSOs. The allocated funds face misappropriation by local authorities and the indirect support of low- and non-democratic regimes, reflected in the affected countries’ drop in indices that measure Social Progress and Democracy. This situation is further aggravated by prioritizing projects that reflect EU-centered agendas and skew the critical aid needed for democracy resilience building. As a response, we bring forward recommendations for the EU to bridge the gap between its democracy policy narrative and execution in Latin America, focusing on the particular needs of the region’s democratic agents.
Peru’s leadership in the Lima Group: emergence and failure (2015–2021)
Authors: Vidarte Arévalo Oscar , Rivas Huerta Andrea
Published: October 2024

This research seeks to understand the factors that enabled Peru, a medium-sized country in Latin America, to take on a leadership role in the creation and development of the Lima Group—a multilateral mechanism whose main objective was to find a resolution to the democratic crisis in Venezuela—as well as the subsequent failure of this leadership. Using the analytical tools provided by Neoclassical Realism, this study argues that Peru’s leadership was made possible not only by the growing importance of the Venezuelan crisis on the international agenda since 2015 but also by changes in regional and global power dynamics. These shifts allowed a country like Peru to exercise such leadership and garner greater interest from the United States. Additionally, the presence of a favorable domestic political climate and clear national interests led the Peruvian government to view this leadership as beneficial, not only for the region but also for Peru itself. However, despite the Venezuelan crisis remaining on the international agenda due to Nicolás Maduro’s continued hold on power, the hardening of the U.S. stance on Venezuela and political changes at the regional level since 2018 fractured the Lima Group and undermined Peru’s leadership. Moreover, domestically, political changes led to heightened instability, weakening Peru’s external position. Finally, the surge in Venezuelan migration and the onset of the COVID-19 pandemic shifted the priorities of Peru’s foreign policy, diminishing its focus on the Venezuelan crisis and contributing to the failure of its leadership.
The impact of informal control on the innovation performance of female technology professionals from the perspective of role pressure
Authors: Zeng Xiangfei , Cao Mengyan , Hu Jingjing , Zhang Wenpei
Published: October 2024

Background

With the rapid advancement of the technology industry, particularly in STEM fields, female professionals have increasingly become key drivers of innovation. Despite this, existing research has seldom examined the psychological impact of informal control on their innovation performance. Therefore, this study distributed questionnaires to female technology professionals in China’s STEM field to investigate the effect of informal control on their innovation performance from a psychological perspective. It further explored the mediating role of role pressure and the moderating role of environmental turbulence in this relationship.

Methods

This study primarily utilized AMOS 24.0 to develop structural equation models, and employed PROCESS 24.0 and SPSS 26.0 for data analysis purposes.

Results

The findings reveal that informal control positively predicted the innovation performance of female technology professionals in STEM field. Role pressure partially mediates this relationship, while environmental turbulence positively moderates the relationship between informal control and innovation performance among female technology professionals in STEM field.

Discussion

Theoretically, this research enriches the individual-level approaches to enhancing management control effectiveness. Practically, it aids managers in focusing on the psychological well-being of female technology professionals in STEM field, thereby facilitating the judicious selection of management control methods. The study’s conclusions aim to provide logical guidance for enterprises to further strengthen their attention and support for female technology professionals in STEM field. Simultaneously, it offers a theoretical foundation for enhancing their innovative capabilities.
Axial rotation affects the cognitive characteristics of spatial ability
Authors: Feng Tian , Zhang Fuchun , Liu Jinzhao , Liang Manqi , Li Yawei
Published: October 2024

Purpose

To test spatial ability in athletes with different axial rotation experience and analyze their behavioral data to explain the cognitive mechanisms of spatial ability in athletes.

Methods

Experiment 1: A total of 147 athletes were selected for the paper-and-pencil mental rotation test (MRT). The athletes were separated according to three sport types: open high-spatial (OH) sport, closed high-spatial (CH) sport, closed low-spatial (CL) sport. Spatial ability testing with a two-factor mixed experimental design of 3 (sport type) × 2 (stimulus type). Experiment 2: In this study, 47 players were selected for computerized mental rotation test, with a three-factor mixed experimental design of 3 (sport type) × 2 (angle: 45°, 90°) × 3 (rotational axis: left–right axis, up–down axis, and front–back axis). Repeated-measures ANOVA was performed to evaluate the data.

Results

(1) The CH group and OH group outperformed the CL group in the non-embodied task (all ps < 0.003) and the CH group was better than the other groups in the embodied and tasks (all ps < 0.008). (2) Under 45° rotational conditions, the reaction time (RT) for the left–right (LR) and up–down (UD) axes were shorter than that for the front-back (FB) axis (all ps < 0.026). However, under 90° conditions, the RT for FB < LR < UD, with superior accuracy and rotational speed for the FB axis than for the LR and UD axes (all ps < 0.034). (3) Male players from the CH and CL groups had shorter RTs than did those from the OH group at both angles (all ps < 0.047). For female players, the CH group presented a shorter RT than the OH and CL groups did at 90° (all ps < 0.006). (4) No sex difference was found for paper and pencil MRTs, but a male advantage existed only in the CL group for computerized MRTs (p = 0.005).

Conclusion

The motor skills associated with axial rotation could promote mental rotation performance and compensate for sex differences in mental rotation ability.
Emotions and interactive tangible tools for math achievement in primary schools
Authors: Saccardo Filippo , Decarli Gisella , Missagia Valentina Irene , Andrao Margherita , Gini Federica , Zancanaro Massimo , Franchin Laura
Published: October 2024

Introduction

Acquiring mathematical concepts is crucial for students’ academic achievements, future prospects and overall well-being. This study explores the role of emotions in a symbolic number comparison task and the impact of the use of a tangible tool.

Methods

Fifty-nine healthy children aged 6 to 7 years participated in a between-subject study with two conditions for the modality, digital tools vs the use of pen and paper, and two conditions for emotions, positive vs neutral.

Results

The study provided evidence that positive emotions can improve task efficiency for pen and paper modality, and the use of the digital tool improves task efficiency with both positive and negative emotions.

Discussion

These findings suggest that addressing emotional factors before engaging in a symbolic task can enhance learning and that interactive technology may give a more significant benefit to students with less positive attitudes toward the task. Incorporating effective teaching methodologies that utilize tangible devices within a positive emotional context can foster engagement and achievement in mathematics, optimizing students’ learning experiences.
Chinese heritage language motivation: a study of motivation development in a multicultural context
Authors: Wen Xiaohong
Published: October 2024

This study investigated motivation of Chinese heritage language (CHL) learners with diverse Chinese language backgrounds at an American university. Using a mixed-methods design, it examined the factors that motivated CHL learners to enroll in Chinese courses and continue their studies. The Study explored interactions that enhanced the learning experience and self identity development. A survey was conducted, followed by individual interviews. The results identified five motivation factors significantly correlated to the ideal L2 self, which functions as an anchor. The ideal L2 self was the strongest predictor of intended effort via positive attitude. The classroom-related experience was another significant predictor of intended effort. Interview data highlighted the dynamic interplay between sociocultural contexts and learner-environment interactions which provokes motivation development and strengthens identity reconstruction and future self-guides. In the process, the learner continuously constructs and consolidates the identity as “Chinese” relating to family and culture. Lastly, the anti-ought-to L2 self, characterized by reactions to “others,” emerged in dynamic interactions between learners and contexts. This motive inspired the learner to continuously develop the possible self and gain positive learning experiences.
Menstrual management in transgender and gender diverse individuals: psychiatric and psychosocial considerations
Authors: Arshed Arslaan , Madanes Sharon , Pottinger Stephanie , Ackerman Marra G. , Deutch Allison B.
Published: October 2024

Transgender and gender-diverse (TGD) menstruators are individuals assigned female at birth (AFAB)*, who retain the capacity to menstruate and have a gender identity that differs from their natal sex. Reports indicate up to 1.6 million individuals in the US identify as TGD. Until recently, the mainstream menstrual discourse has failed to capture the experience of transmenstruators. However, a better understanding of the menstrual experiences of TGD-AFAB will allow for more individualized patient-centered care. In this review, we provide the relevant data necessary to inform the psychiatric management of menstruation in TGD-AFAB individuals, including experiences of menstruation, preferences for menstrual management, and the impact on mental health. Our review indicates that menstrual care in TGD patients must be tailored to the individual; clinicians should remain open-minded to the unique experience of transmenstruators; gender-affirming menstrual care is necessary to reduce psychological burden. It should not be assumed that TGD-AFAB menstruators are utilizing appropriate contraceptive methods and should receive contraceptive and fertility preservation counseling. We highlight the importance of having these conversations early in the reproductive arch, even before puberty onset. Keeping in mind the gender minority stress model, in the upcoming sections, we discuss the limited body of literature on mood disorders in TGD-AFAB individuals who menstruate, undergo menstrual suppression, or continue to ovulate. The psychological impact of hormonal therapies is also reviewed.
Does radiofrequency radiation impact sleep? A double-blind, randomised, placebo-controlled, crossover pilot study
Authors: Bijlsma Nicole , Conduit Russell , Kennedy Gerard , Cohen Marc
Published: October 2024

The most common source of Radiofrequency Electromagnetic Field (RF-EMF) exposures during sleep includes digital devices, yet there are no studies investigating the impact of multi-night exposure to electromagnetic fields emitted from a baby monitor on sleep under real-world conditions in healthy adults. Given the rise in the number of people reporting to be sensitive to manmade electromagnetic fields, the ubiquitous use of Wi-Fi enabled digital devices and the lack of real-world data, we investigated the effect of 2.45 GHz radiofrequency exposure during sleep on subjective sleep quality, and objective sleep measures, heart rate variability and actigraphy in healthy adults. This pilot study was a 4-week randomised, double-blind, crossover trial of 12 healthy adults. After a one-week run-in period, participants were randomised to exposure from either an active or inactive (sham) baby monitor for 7 nights and then crossed over to the alternate intervention after a one-week washout period. Subjective and objective assessments of sleep included the Pittsburgh Insomnia Rating Scale (PIRS-20), electroencephalography (EEG), actigraphy and heart rate variability (HRV) derived from electrocardiogram. Sleep quality was reduced significantly (p < 0.05) and clinically meaningful during RF-EMF exposure compared to sham-exposure as indicated by the PIRS-20 scores. Furthermore, at higher frequencies (gamma, beta and theta bands), EEG power density significantly increased during the Non-Rapid Eye Movement sleep (p < 0.05). No statistically significant differences in HRV or actigraphy were detected. Our findings suggest that exposure to a 2.45 GHz radiofrequency device (baby monitor) may impact sleep in some people under real-world conditions however further large-scale real-world investigations with specified dosimetry are required to confirm these findings.
Predicting humor effectiveness of robots for human line cutting
Authors: Ushijima Yuto , Satake Satoru , Kanda Takayuki
Published: October 2024

It is extremely challenging for security guard robots to independently stop human line-cutting behavior. We propose addressing this issue by using humorous phrases. First, we created a dataset and built a humor effectiveness predictor. Using a simulator, we replicated 13,000 situations of line-cutting behavior and collected 500 humorous phrases through crowdsourcing. Combining these simulators and phrases, we evaluated each phrase’s effectiveness in different situations through crowdsourcing. Using machine learning with this dataset, we constructed a humor effectiveness predictor. In the process of preparing this machine learning, we discovered that considering the situation and the discomfort caused by the phrase is crucial for predicting the effectiveness of humor. Next, we constructed a system to select the best humorous phrase for the line-cutting behavior using this predictor. We then conducted a video experiment in which we compared the humorous phrases selected using this proposed system with typical non-humorous phrases. The results revealed that humorous phrases selected by the proposed system were more effective in discouraging line-cutting behavior than typical non-humorous phrases.
The Schatten current sheet
Authors: Knizhnik Kalman J.
Published: October 2024

Space weather models endeavoring to connect remote observations to in-situ measurements at various locations in the heliosphere invariably require a coronal model to connect the photosphere magnetically to the inner heliosphere. The most famous and popular implementation of this connection is a potential field source surface (PFSS) model out to the source surface, typically located at 2.5 solar radii, combined with a Schatten current sheet (SCS) model. While the PFSS model is mostly understood, the SCS has been utilized in heliospheric physics for nearly 50 years with little understanding of it’s physical and mathematical underpinnings. In this overview article, I lay out the mathematical formalism of the SCS, describe how it differs from the PFSS, and summarize several techniques used to combine the PFSS and SCS to create a global coronal model from the photosphere to the inner heliosphere.
Advanced quantitative magnetic resonance imaging of lower extremity muscle microtrauma after marathon: a mini review
Authors: Cheng Yu , Li Xiaokai
Published: October 2024

This article reviews the existing literature and outlines recent advances in quantitative Magnetic Resonance Imaging (MRI) techniques for the assessment of lower extremity muscle microtrauma following a marathon. Single-modality quantitative MRI techniques include T2 mapping to assess the dynamics of muscle inflammatory edema and variability at the site of injury, Diffusion Tensor Imaging (DTI) to detect subclinical changes in muscle injury, Intravoxel Incoherent Motion (IVIM) imaging to provide simultaneous information on perfusion and diffusion in muscle tissue without the need for intravenous contrast, and Magnetic Resonance Spectroscopy (MRS) to noninvasively detect intramyocellular lipid (IMCL) content in muscle before and after marathon exercise to explain the use of fatty acids as an energy source in skeletal muscle during long-distance running. As well as Chemical Exchange Saturation Transfer (CEST) is particularly suitable for detecting changes in free creatine, pH values and lactate concentrations in muscles before and after exercise, providing a more detailed picture of muscle physiology and chemistry. These metabolic MRI methods enhance the understanding of biochemical alterations occurring in muscles pre- and post-exercise. Multimodal techniques combine different modalities to provide a comprehensive evaluation of muscle structural and functional changes. These advanced techniques aim to better assess microtrauma and guide clinical treatment, though further validation with larger studies is needed to establish their potential over traditional qualitative methods.