bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Ghrelin on the Maturation of Sheep Oocytes and Early Embryonic Development In Vitro
Authors: Wang Daqing , Yang Yanyan , Song Yongli , Fu Shaoyin , He Xiaolong , Wang Biao , Wang Liwei , Chen Xin , Li Xihe , Liu Yongbin , Cao Guifang
Published: April 2022

In vitro maturation (IVM) of sheep oocytes and early embryonic development are of great scientific importance for the study of reproductive development in sheep. Ghrelin is an important hormone that regulates the secretion of the growth hormone (GH). In this study, different gradients of ghrelin (0, 100, 200, and 300 ng/mL) were added to the IVM system of sheep oocytes to observe their cell morphology, and Hosesth 33342 staining was used to determine the time taken for oocytes to reach different developmental stages. We found 200 ng/mL ghrelin to be the optimal concentration. The RNA-seq analysis showed that many signaling pathways were significantly altered by ghrelin. Cell cycle, Wnt, and oxidative phosphorylation were activated; the P53 was inhibited. These pathways together regulate the maturation of oocytes and early embryonic development in vitro. The effects of the addition of ghrelin were verified by the expression of GLUT1 in early embryonic development. The results suggest that adding ghrelin shortens the duration of the IVM of sheep oocytes and hinders early embryonic development. This study provides new insights into the effects of exogenous ghrelin on sheep oocyte maturation and early embryonic development in vitro.
Staphylococcus aureus Causing Skin and Soft Tissue Infections in Companion Animals: Antimicrobial Resistance Profiles and Clonal Lineages
Authors: Costa Sofia Santos , Ribeiro Rute , Serrano Maria , Oliveira Ketlyn , Ferreira Carolina , Leal Marta , Pomba Constança , Couto Isabel
Published: April 2022

Staphylococcus aureus is a relevant agent of skin and soft tissue infections (SSTIs) in animals. Fifty-five S. aureus comprising all SSTI-related isolates in companion animals, collected between 1999 and 2018 (Lab 1) or 2017 and 2018 (Lab 2), were characterized regarding susceptibility to antibiotics and heavy metals and carriage of antimicrobial resistance determinants. Clonal lineages were established by PFGE, MLST and agr typing. Over half of the isolates (56.4%, 31/55) were methicillin-resistant S. aureus (MRSA), and 14.5% showed a multidrug resistance (MDR) phenotype. Resistance was most frequently observed for beta-lactams (81.8%, related to blaZ and/or mecA), fluoroquinolones (56.4%) and macrolides/lincosamides (14.5%, related to erm(A) or erm(C)). The distributions of heavy-metal MICs allowed the detection of non-wild-type populations associated with several resistance genes. The collection showed genetic diversity, with prevalence of clonal lineage ST22-agrI (45.5%, 25/55), comprising only MRSA isolates, and several less frequently detected clones, including ST5-agrII (14.6%, 8/55), ST398-agrI (9.1%, 5/55) and ST72-agrI (7.3%, 4/55). This work highlights the high frequency of SSTI-related MRSA strains that reflect the clonal lineages circulating both in companion animals and humans in Portugal, reinforcing the need for a One Health approach when studying staphylococci causing infections in companion animals.
Bacteriophage-Enriched Galenic for Intrapericardial Ventricular Assist Device Infection
Authors: Rojas Sebastian V. , Junghans Simon , Fox Henrik , Lazouski Kanstantsin , Schramm Rene , Morshuis Michiel , Gummert Jan F. , Gross Justus
Published: April 2022

We report a case of severe outflow graft infection following left ventricular assist device (LVAD) implantation. A 51-year old male LVAD patient was readmitted to our hospital presenting signs of systemic infection. One year previously, LVAD implantation (HeartMate3, Abbott, Chicago, IL, USA) with concomitant patent foramen ovale closure had been performed in the context of end-stage heart failure due to dilative cardiomyopathy (INTERMACS III). The indication for LVAD-therapy was bridge-to-candidacy, since the patient did not instantly fulfill all criteria for cardiac transplantation. At admission, a PET-CT scan unveiled fluid accumulation, encircling the outflow-graft prosthesis (SUVmax 10.5) with contrast-enhancement involving the intrathoracic driveline (SUVmax 11.2). Since cardiac transplantation was not feasible, the patient underwent surgical revision. In the first step, redo sternotomy was performed with local debridement, including jet lavage. Intraoperative swabs confirmed bacterial infection with staphylococcus aureus. Following this, the patient underwent negative pressure wound therapy (NPWT) with instillation using the V.A.C. VERAFLO system (KCI-3M, San Antonio, TX, USA) for a total of 19 days. Due to the severity of infection, local bacteriophage application was performed within the wound closure. In order to concentrate phage therapy at the infection site, phages were applied using a novel semi-fluid galenic. After wound closure, the patient was discharged with an uneventful course. A control PET-CT scan 3 months after discharge showed a significant decrease in infection (outflow graft: SUVmax 7.2, intrathoracic driveline: SUVmax 3.0) correlated with contrast enhancement. Bacterial infection of intrathoracic VAD components represents a severe and potentially life-threatening complication. If cardiac transplantation is not feasible, complex wound management strategies are required. Local bacteriophage therapy might be a promising addition to already established therapeutical options. In order to improve bacteriophage retention at the wound site, application of a viscous galenic might be beneficial.
DDAH1 Protects against Acetaminophen-Induced Liver Hepatoxicity in Mice
Authors: Shen Xiyue , Ishaq Saddam Muhammad , Wang Qiao’e , Yuan Juntao , Gao Junling , Lu Zhongbing
Published: April 2022

In many developed countries, acetaminophen (APAP) overdose-induced acute liver injury is a significant therapeutic problem. Dimethylarginine dimethylaminohydrolase 1 (DDAH1) is a critical enzyme for asymmetric dimethylarginine (ADMA) metabolism. Growing evidence suggests that liver dysfunction is associated with increased plasma ADMA levels and reduced hepatic DDAH1 activity/expression. The purpose of this study was to investigate the involvement of DDAH1 in APAP-mediated hepatotoxicity using Ddah1-/- and DDAH1 transgenic mice. After APAP challenge, Ddah1-/- mice developed more severe liver injury than wild type (WT) mice, which was associated with a greater induction of fibrosis, oxidative stress, inflammation, cell apoptosis and phosphorylation of JNK. In contrast, overexpression of DDAH1 attenuated APAP-induced liver injury. RNA-seq analysis showed that DDAH1 affects xenobiotic metabolism and glutathione metabolism pathways in APAP-treated livers. Furthermore, we found that DDAH1 knockdown aggravated APAP-induced cell death, oxidative stress, phosphorylation of JNK and p65, upregulation of CYP2E1 and downregulation of GSTA1 in HepG2 cells. Collectively, our data suggested that DDAH1 has a marked protective effect against APAP-induced liver oxidative stress, inflammation and injury. Strategies to increase hepatic DDAH1 expression/activity may be novel approaches for drug-induced acute liver injury therapy.
Antioxidant and Wound Healing Potential of Vitis vinifera Seeds Supported by Phytochemical Characterization and Docking Studies
Authors: Al-Warhi Tarfah , Zahran Eman Maher , Selim Samy , Al-Sanea Mohammad M. , Ghoneim Mohammed M. , Maher Sherif A. , Mostafa Yaser A. , Alsenani Faisal , Elrehany Mahmoud A. , Almuhayawi Mohammed S. , Al Jaouni Soad K. , Abdelmohsen Usama Ramadan , Elmaidomy Abeer H.
Published: April 2022

This study explored the in vivo wound healing potential of Vitis vinifera seed extract using an excision wound model with focus on wound healing molecular targets including TGFBR1, VEGF, TNF-α, and IL-1β. The wound healing results revealed that V. vinifera seed extract enhanced wound closure rates (p < 0.001), elevated TGF-β and VEGF levels, and significantly downregulated TNF-α and IL-1β levels in comparison to the Mebo®-treated group. The phenotypical results were supported by biochemical and histopathological findings. Phytochemical investigation yielded a total of 36 compounds including twenty-seven compounds (1–27) identified from seed oil using GC-MS analysis, along with nine isolated compounds. Among the isolated compounds, one new benzofuran dimer (28) along with eight known ones (29–36) were identified. The structure of new compound was elucidated utilizing 1D/2D NMR, with HRESIMS analyses. Moreover, molecular docking experiments were performed to elucidate the molecular targets (TNF-α, TGFBR1, and IL-1β) of the observed wound healing activity. Additionally, the in vitro antioxidant activity of V. vinifera seed extract along with two isolated compounds (ursolic acid 34, and β-sitosterol-3-O-glucopyranoside 36) were explored. Our study highlights the potential of V. vinifera seed extract in wound repair uncovering the most probable mechanisms of action using in silico analysis.
The Vascular Niche for Adult Cardiac Progenitor Cells
Authors: Herrero Diego , Albericio Guillermo , Higuera Marina , Herranz-López María , García-Brenes Miguel A. , Cordero Alejandra , Roche Enrique , Sepúlveda Pilar , Mora Carmen , Bernad Antonio
Published: April 2022

Research on cardiac progenitor cell populations has generated expectations about their potential for cardiac regeneration capacity after acute myocardial infarction and during physiological aging; however, the endogenous capacity of the adult mammalian heart is limited. The modest efficacy of exogenous cell-based treatments can guide the development of new approaches that, alone or in combination, can be applied to boost clinical efficacy. The identification and manipulation of the adult stem cell environment, termed niche, will be critical for providing new evidence on adult stem cell populations and improving stem-cell-based therapies. Here, we review and discuss the state of our understanding of the interaction of adult cardiac progenitor cells with other cardiac cell populations, with a focus on the description of the B-CPC progenitor population (Bmi1+ cardiac progenitor cell), which is a strong candidate progenitor for all main cardiac cell lineages, both in the steady state and after cardiac damage. The set of all interactions should be able to define the vascular cardiac stem cell niche, which is associated with low oxidative stress domains in vasculature, and whose manipulation would offer new hope in the cardiac regeneration field.
Study on the Neuroprotective, Radical-Scavenging and MAO-B Inhibiting Properties of New Benzimidazole Arylhydrazones as Potential Multi-Target Drugs for the Treatment of Parkinson’s Disease
Authors: Anastassova Neda , Aluani Denitsa , Hristova-Avakumova Nadya , Tzankova Virginia , Kondeva-Burdina Magdalena , Rangelov Miroslav , Todorova Nadezhda , Yancheva Denitsa
Published: April 2022

Oxidative stress is a key contributing factor in the complex degenerating cascade in Parkinson’s disease. The inhibition of MAO-B affords higher dopamine bioavailability and stops ROS formation. The incorporation of hydroxy and methoxy groups in the arylhydrazone moiety of a new series of 1,3-disubstituted benzimidazole-2-thiones could increase the neuroprotective activity. In vitro safety evaluation on SH-SY5Y cells and rat brain synaptosomes showed a strong safety profile. Antioxidant and neuroprotective effects were evaluated in H2O2-induced oxidative stress on SH-SY5Y cells and in a model of 6-OHDA-induced neurotoxicity in rat brain synaptosomes, where the dihydroxy compounds 3h and 3i demonstrated the most robust neuroprotective and antioxidant activity, more pronounced than the reference melatonin and rasagiline. Statistically significant MAO-B inhibitory effects were exerted by some of the compounds where again the catecholic compound 3h was the most potent inhibitor similar to selegiline and rasagiline. The most potent antioxidant effect in the ferrous iron induced lipid peroxidation assay was observed for the three catechols—3h and 3j, 3q. The catecholic compound 3h showed scavenging capability against superoxide radicals and antioxidant effect in the iron/deoxyribose system. The study outlines a perspective multifunctional compound with the best safety profile, neuroprotective, antioxidant and MAO-B inhibiting properties.
Links between Disease Severity, Bacterial Infections and Oxidative Stress in Cystic Fibrosis
Authors: Galiniak Sabina , Mołoń Mateusz , Rachel Marta
Published: April 2022

Cystic fibrosis (CF) is one of the most common, yet fatal genetic diseases in Caucasians. The presence of a defective CF transmembrane conductance regulator and the massive neutrophils influx into the airways contribute to an imbalance in epithelial cell processes and extracellular fluids and lead to excessive production of reactive oxygen species and intensification of oxidative stress. The study included 16 controls and 42 participants with CF aged 10 to 38. The products of protein oxidation, total antioxidant capacity (TAC) and markers of lipid peroxidation were estimated in the serum of the subjects. Furthermore, we compared the level of oxidative stress in patients with CF according to the severity of disease and type of bacterial infection. Thiol groups and serum TAC decreased significantly in patients with CF (p < 0.05). Elevated levels of 3-nitrotyrosine, malondialdehyde and 8-isoprostane were observed in CF subjects (p < 0.05). Furthermore, as the severity of the disease increased, there was a decrease in the thiol groups and TAC levels, as well as an increase in the concentration of 3-nitrotyrosine and 8-isoprostane. CF participants infected with Pseudomonas aeruginosa had elevated 3-nitrotyrosine concentration levels (p < 0.05), while those infected with Staphylococcus aureus noted a decrease in thiol groups (p < 0.05). Elevated levels of oxidative stress markers were found in the serum of CF patients. Furthermore, oxidative stress progressively increased over the years and along with the severity of the disease. The presence of bacterial infection with P. aeruginosa or S. aureus had a slight effect on oxidative stress, while co-infection by two species did not affect the level of oxidative stress.
Ocular Hypertension Results in Hypoxia within Glia and Neurons throughout the Visual Projection
Authors: Jassim Assraa Hassan , Nsiah Nana Yaa , Inman Denise M.
Published: April 2022

The magnitude and duration of hypoxia after ocular hypertension (OHT) has been a matter of debate due to the lack of tools to accurately report hypoxia. In this study, we established a topography of hypoxia in the visual pathway by inducing OHT in mice that express a fusion protein comprised of the oxygen-dependent degradation (ODD) domain of HIF-1α and a tamoxifen-inducible Cre recombinase (CreERT2) driven by a ubiquitous CAG promoter. After tamoxifen administration, tdTomato expression would be driven in cells that contain stabilized HIF-1α. Intraocular pressure (IOP) and visual evoked potential (VEP) were measured after OHT at 3, 14, and 28 days (d) to evaluate hypoxia induction. Immunolabeling of hypoxic cell types in the retina and optic nerve (ON) was performed, as well as retinal ganglion cell (RGC) and axon number quantification at each time point (6 h, 3 d, 14 d, 28 d). IOP elevation and VEP decrease were detected 3 d after OHT, which preceded RGC soma and axon loss at 14 and 28 d after OHT. Hypoxia was detected primarily in Müller glia in the retina, and microglia and astrocytes in the ON and optic nerve head (ONH). Hypoxia-induced factor (HIF-α) regulates the expression of glucose transporters 1 and 3 (GLUT1, 3) to support neuronal metabolic demand. Significant increases in GLUT1 and 3 proteins were observed in the retina and ON after OHT. Interestingly, neurons and endothelial cells within the superior colliculus in the brain also experienced hypoxia after OHT as determined by tdTomato expression. The highest intensity labeling for hypoxia was detected in the ONH. Initiation of OHT resulted in significant hypoxia that did not immediately resolve, with low-level hypoxia apparent out to 14 and 28 d, suggesting that continued hypoxia contributes to glaucoma progression. Restricted hypoxia in retinal neurons after OHT suggests a hypoxia management role for glia.
Diketo-Pyrrolo Pyrrole-Based Acceptor-Acceptor Copolymers with Deep HOMO and LUMO Levels Absorbing in the Near Infrared
Authors: Khelifi Wissem , Awada Hussein , Blanc Sylvie , Roche Gilles Henri , Hirsch Lionel , Oboho Bassey , Castet Frédéric , Bousquet Antoine , Lartigau-Dagron Christine
Published: April 2022

A series of acceptor-acceptor (A-A’) alternated copolymers based on dithienodiketopyrrolo pyrrole were synthesized by copolymerizing it with itself and other different electron-poor monomers. The experimental and computed optoelectronic properties of four DPP-based copolymers, P(DPP-DPP) (with linear and branched chains), copolymer with diazapentalene P(DPP-DAP) and also with dioxothienopyrrolebenzodifurandione P(DPP-BTPBF), as well as thermal characterizations were described. UV-visible spectrophotometry and cyclic voltammetry were used to estimate the optical and electrochemical bandgaps, and were found as very small: 1.3, 1.0, and 0.9 eV for P(DPP-DPP), P(DPP-DAP), and P(DPP-BTPBF), respectively. The BTPBF unit allowed a strong reduction of the bandgap, leading to a broad absorption in the visible and near infra-red regions from 650 to 1450 nm. These results were compared to analogous donor-acceptor (D-A) copolymers previously reported, in which DPP is replaced by DTS, P(DTS-DPP), P(DTS-DAP), and P(DTS-BTPBF). The same trend was observed. By comparing A-A’ to D-A’ copolymers analogues, it was shown that the bandgap remained the same while both HOMO and LUMO levels were lowered by roughly 0.2 eV.
Effects of Magnetic Stimulation on Dental Implant Osseointegration: A Scoping Review
Authors: Cecoro Gennaro , Bencivenga Debora , Annunziata Marco , Cennamo Nunzio , Della Ragione Fulvio , Formisano Alessandro , Piccirillo Angelantonio , Stampone Emanuela , Volpe Pio Antonio , Zeni Luigi , Borriello Adriana , Guida Luigi
Published: April 2022

This PRISMA-ScR driven scoping review aims to evaluate the influence of magnetic field stimulation on dental implant osseointegration. Seven databases were screened adopting ad-hoc strings. All clinical and preclinical studies analyzing the effects of magnetic fields on dental implant osseointegration were included. From 3124 initial items, on the basis of the eligibility criteria, 33 articles, regarding both Pulsed ElectroMagnetic Fields (PEMF) and Static magnetic Fields from permanent Magnets (SFM) were finally included and critically analyzed. In vitro studies showed a positive effect of PEMF, but contrasting effects of SFM on bone cell proliferation, whereas cell adhesion and osteogenic differentiation were induced by both types of stimulation. In vivo studies showed an increased bone-to-implant contact rate in different animal models and clinical studies revealed positive effects on implant stability, under magnetic stimulation. In conclusion, although positive effects of magnetic exposure on osteogenesis activity and osseointegration emerged, this scoping review highlighted the need for further preclinical and clinical studies. More standardized designs, accurate choice of stimulation parameters, adequate methods of evaluation of the outcomes, greater sample size and longer follow-ups are needed to clearly assess the effect of magnetic fields on dental implant osseointegration.
Editorial of the Special Issue “Statistics and Pattern Recognition Applied to the Spatio-Temporal Properties of Seismicity”
Authors: Gentili Stefania , Di Giovambattista Rita , Shcherbakov Robert , Vallianatos Filippos
Published: April 2022

Due to the significant increase in the availability of new data in recent years, as a result of the expansion of available seismic stations, laboratory experiments, and the availability of increasingly reliable synthetic catalogs, considerable progress has been made in understanding the spatiotemporal properties of earthquakes [...]
Form Finding of Asymmetric Cable Network Reflector with Flexible Ring Truss
Authors: Hu Zifan , Luo Yajun , Zhang Xinong
Published: April 2022

In this work, the form finding of an asymmetric cable network reflector is considered by taking into account the elastic deformation of the flexible ring truss. Firstly, by treating the boundary nodes as fixed nodes, the balance equations of the structure are transformed into linear equations using the force density method (FDM). Next, the genetic algorithm (GA) is used to find the force density values that satisfy the surface accuracy required to complete the form finding of a cable network reflector. Secondly, a design procedure is presented by incorporating the elastic deformation of the ring truss. Finally, using an iterative process, the coordinates of the boundary nodes are modified to repeat the first step to obtain better surface accuracy. Numerical experiments are presented to demonstrate the effectiveness of the proposed method in comparison with the conventional method.
Spray-Drying Hen Eggs: Effects of the Egg Yolk to Egg White Ratio and Sucrose Addition on the Physicochemical, Functional, and Nutritional Properties of Dried Products and on Their Amino Acid Profiles
Authors: Vargas-del-Río Liliana M. , García-Figueroa Alexis , Fernández-Quintero Alejandro , Rodríguez-Stouvenel Aida
Published: April 2022

Manufactured egg powders can be formulated to produce food products that vary in their properties. The present study aims to determine the effect of egg white content on the physicochemical characteristics and on the functionality and nutritional value of dried whole egg (WE), egg white (W), and an egg yolk/white mixture in a 1:3 ratio (M1:3). These fresh egg products were spray-dried using sucrose—an agent recognized for its effect of protecting the protein in the egg during the drying process. Experiments were conducted in a laboratory-scale spray dryer, operated under controlled conditions, with an air inlet temperature of 120 °C. In the dried products, water activity, water solubility index, color, and pH were affected significantly as a function of the fresh egg component ratio and the added sucrose. The wettability and dispersibility in water of egg powder were improved when sucrose was added to the WE. The water-holding capacity was highest in dried egg white, and higher in the M1:3 mixture without sucrose added than in the WE with 5% sucrose. The results suggest that modifying the ratio of fresh egg yolk to egg white could lend some control over the protein and fat contents of dried egg products and over their functional properties.
Ultrasound Localization Microscopy in Liquid Metal Flows
Authors: Weik David , Grüter Lars , Räbiger Dirk , Singh Sanjay , Vogt Tobias , Eckert Sven , Czarske Jürgen , Büttner Lars
Published: April 2022

Liquid metal convection plays an important role in natural and technical processes. In experimental studies, an instrumentation with a sub-millimeter spatial resolution is required in an opaque fluid to resolve the flow field near the boundary layer. Using ultrasound methods, the trade-off between the frequency and imaging depth of typical laboratory experiments limits the spatial resolution. Therefore, the method of ultrasound localization microscopy (ULM) was introduced in liquid metal experiments for the first time in this study. To isolate the intrinsic scattering particles, an adaptive nonlinear beamformer was applied. As a result, an average spatial resolution of 188 μm could be achieved, which corresponded to a fraction of the ultrasound wavelength of 0.28. A convection experiment was measured using ULM. Due to the increased spatial resolution, the high-velocity gradients and the recirculation areas of a liquid metal convection experiment could be observed for the first time. The presented technique paves the way for in-depth flow studies of convective turbulent liquid metal flows that are close to the boundary layer.
Method of Planning Repairs of the Installation including Building Waste
Authors: Nowogońska Beata , Nowogoński Ireneusz
Published: April 2022

Repairs of water supply, sewage and central heating installations in residential buildings should be carried out systematically. However, very often, renovation dates are postponed, which results in installation failures. The failures of water supply, sewage and central heating installations, due to the currently used methods of masking them and running them as under-plaster and under-floor installations, are always connected with the damage and necessity of reconstruction of the building elements. As a result, renovation work has to be carried out to a greater extent and the amount of construction waste is much greater. The analysis of different renovation strategies of water supply, sewage and central heating systems in residential buildings made in traditional technology has been carried out. The article presents the results of the research on the effects of the postponement of the renovation works on the changes in the technical condition of the building and on the scope of renovation works. The aim of the research is to develop a method for planning repairs of the installation taking into account optimization of the amount of construction waste. The aim of the research is also to answer the question: To what extent does the postponed repair of water and sewage installations influence the amount of construction waste? In the proposed method, the Prediction of Reliability according to Rayleigh Distribution (PRRD) model is used. The results of the research indicate the necessity of conducting the renovation works of the installation in a timely manner due to the increasing amount of construction waste and the introduced reduction of its amount with the increase of the recycling rate.
Intelligent Task Offloading in Fog Computing Based Vehicular Networks
Authors: Alvi Ahmad Naseem , Javed Muhammad Awais , Hasanat Mozaherul Hoque Abul , Khan Muhammad Badruddin , Saudagar Abdul Khader Jilani , Alkhathami Mohammed , Farooq Umar
Published: April 2022

Connected vehicles in vehicular networks will lead to a smart and autonomous transportation system. These vehicles have a large number of applications that require wireless connectivity by using cellular vehicle-to-everything (C-V2X). The infrastructure of C-V2X comprises multiple roadside units (RSUs) that provide direct connectivity with the on-road vehicles. Vehicular traffic applications are mainly categorized into three major groups such as emergency response traffic, traffic management and infotainment traffic. Vehicles have limited processing capabilities and are unable to process all tasks simultaneously. To process these offloaded tasks in a short time, fog servers are placed near the RSUs. However, it is sometimes not possible for the fog computing server to process all offloaded tasks. In this work, a utility function for the RSU to process these offloaded tasks is designed. In addition, a knapsack-based task scheduling algorithm is proposed to optimally process the offloaded tasks. The results show that the proposed scheme helps fog nodes to optimally scrutinize the high-priority offloaded tasks for task execution resulting in more than 98% of emergency tasks beingprocessed by fog computing nodes.
Comparative Study of Multiclass Text Classification in Research Proposals Using Pretrained Language Models
Authors: Lee Eunchan , Lee Changhyeon , Ahn Sangtae
Published: April 2022

Recently, transformer-based pretrained language models have demonstrated stellar performance in natural language understanding (NLU) tasks. For example, bidirectional encoder representations from transformers (BERT) have achieved outstanding performance through masked self-supervised pretraining and transformer-based modeling. However, the original BERT may only be effective for English-based NLU tasks, whereas its effectiveness for other languages such as Korean is limited. Thus, the applicability of BERT-based language models pretrained in languages other than English to NLU tasks based on those languages must be investigated. In this study, we comparatively evaluated seven BERT-based pretrained language models and their expected applicability to Korean NLU tasks. We used the climate technology dataset, which is a Korean-based large text classification dataset, in research proposals involving 45 classes. We found that the BERT-based model pretrained on the most recent Korean corpus performed the best in terms of Korean-based multiclass text classification. This suggests the necessity of optimal pretraining for specific NLU tasks, particularly those in languages other than English.
An Eco-Driving Controller Based on Intelligent Connected Vehicles for Sustainable Transportation
Authors: Wang Pangwei , Ye Rongsheng , Zhang Juan , Wang Tianren
Published: April 2022

The rapid increase in the number of vehicles has brought significant challenges to energy conservation and environmental sustainability. To solve these problems, various frameworks and models based on intelligent connected vehicles (ICVs) have been identified for road capacity improvement and fuel consumption reduction. In this paper, an eco-driving controller with ICVs was first proposed by combining vehicular dynamics with wireless communication technologies, where the nodes that can implement perception and control in a simulated complex traffic environment have been deployed. Then, the information of the surrounding environment, including the preceding vehicles, was obtained through a wireless communication module based on the technology of vehicle to everything (V2X). Besides, the advanced model predictive control (MPC) strategy was integrated into the ICV controller with the objectives of minimizing the driving spacing and improving environmental sustainability. Finally, a co-simulation platform for ICVs based on a robot operating system (ROS) and PreScan software was constructed, and the dynamic characteristics of the controller were verified in three aspects, including car-following behaviors, fuel efficiency improvement, and carbon dioxide emission reduction. The proposed controller showed that it can reduce fuel consumption by 3.71% and reduce carbon dioxide emissions by 3.42%, in the scenarios of a preceding vehicle with constant velocity, and by 6.77% and 7.91%, respectively, in a preceding vehicle with variable velocity scenario. The demonstrated experimental results show that the proposed controller can effectively reduce fuel consumption and emissions during car-following.
Deep-Learning-Based Stream-Sensing Method for Detecting Asynchronous Multiple Signals
Authors: Kim Yeongjun , Lee Harim
Published: April 2022

In a disaster site, terrestrial communication infrastructures are often destroyed or malfunctioning, and hence it is very difficult to detect the existence of survivors in the site. At such sites, UAVs are rapidly emerging as an alternative to mobile base stations to establish temporary infrastructure. In this paper, a novel deep-learning-based multi-source detection scheme is proposed for the scenario in which an UAV wants to estimate the number of survivors sending rescue signals within its coverage in a disaster site. For practicality, survivors are assumed to use off-the-shelf smartphones to send rescue signals, and hence the transmitted signals are orthogonal frequency division multiplexing (OFDM)-modulated. Since the line of sight between the UAV and survivors cannot be generally secured, the sensing performance of existing radar techniques significantly deteriorates. Furthermore, we discover that transmitted signals of survivors are unavoidably aysnchronized to each other, and thus existing frequency-domain multi-source classification approaches cannot work. To overcome the limitations of these existing technologies, we propose a lightweight deep-learning-based multi-source detection scheme by carefully designing neural network architecture, input and output signals, and a training method. Extensive numerical simulations show that the proposed scheme outperforms existing methods for various SNRs under the scenario where synchronous and asynchronous transmission is mixed in a received signal. For almost all cases, the precision and recall of the proposed scheme is nearly one, even when users’ signal-to-noise ratios (SNRs) are randomly changing within a certain range. The precision and recall are improved up to 100% compared to existing methods, confirming that the proposal overcomes the limitation of the existing works due to the asynchronicity. Moreover, for Intel(R) Core(TM) i7-6900K CPU, the processing time of our proposal for a case is 31.8 milliseconds. As a result, the proposed scheme provides a robust and reliable detection performance with fast processing time. This proposal can also be applied to any field that needs to detect the number of wireless signals in a scenario where synchronization between wireless signals is not guaranteed.
Ajwa-Dates (Phoenix dactylifera)-Mediated Synthesis of Silver Nanoparticles and Their Anti-Bacterial, Anti-Biofilm, and Cytotoxic Potential
Authors: Allemailem Khaled S. , Khadri Habeeb , Azam Mohd , Khan Masood Alam , Rahmani Arshad Husain , Alrumaihi Faris , Khateef Riazunnisa , Ansari Mohammad Azam , Alatawi Eid A. , Alsugoor Mahdi H. , Almansour Nahlah Makki , Alhatlani Bader Y. , Almatroudi Ahmad
Published: April 2022

Green nanotechnology is the evolution of cost-effective and environmentally friendly processes for the production of metal-based nanoparticles due to medicinal importance and economic value. The aim of the present study was to biosynthesize and characterize silver nanoparticles (AgNPs) using the seed extract of Ajwa dates (Aw). The anti-bacteriostatic activity of biosynthesized Aw–AgNPs against Gram-positive and Gram-negative bacterial strains was evaluated. The anti-biofilm activity was examined by the tissue culture plate method. Lastly, the anti-cancer potential of Aw–AgNPs was investigated against the human breast cancer cell line HCC712. UV–visible absorption spectra exhibited the plasmon resonance peak at 430 nm, with the solution undergoing rapid color changes that verified the existence of biosynthesized silver nanoparticles in the solution. TEM and SEM images illustrated that the Aw–AgNPs were spherical and between 15 and 80 nm in diameter. The reduction and stabilization of Aw–AgNPs was due to the functional groups present in the biomolecules of the Ajwa seeds, as identified by FTIR. The Aw–AgNPs exhibited significant anti-bacterial activity against all the tested bacterial strains. Moreover, the Aw–AgNPs efficiently hampered the biofilm formation of the bacterial strains and exhibited cytotoxicity at various concentrations. Overall, these findings suggest that biosynthesized Aw–AgNPs may be used as a potential therapeutic formulation against bacterial infections and breast cancer.
Clustering of Road Traffic Accidents as a Gestalt Problem
Authors: Gnjatović Milan , Košanin Ivan , Maček Nemanja , Joksimović Dušan
Published: April 2022

This paper introduces and illustrates an approach to automatically detecting and selecting “critical” road segments, intended for application in circumstances of limited human or technical resources for traffic monitoring and management. The reported study makes novel contributions at three levels. At the specification level, it conceptualizes “critical segments” as road segments of spatially prolonged and high traffic accident risk. At the methodological level, it proposes a two-stage approach to traffic accident clustering and selection. The first stage is devoted to spatial clustering of traffic accidents. The second stage is devoted to selection of clusters that are dominant in terms of number of accidents. At the implementation level, the paper reports on a prototype system and illustrates its functionality using publicly available real-life data. The presented approach is psychologically inspired to the extent that it introduces a clustering criterion based on the Gestalt principle of proximity. Thus, the proposed algorithm is not density-based, as are most other state-of-the-art clustering algorithms applied in the context of traffic accident analysis, but still keeps their main advantages: it allows for clusters of arbitrary shapes, does not require an a priori given number of clusters, and excludes “noisy” observations.
Investigation on Functional, Thermo-Mechanical and Bread-Making Properties of Some White and Black Rice Flours
Authors: Banu Iuliana , Aprodu Iuliana
Published: April 2022

The flour from white rice, Arborio (ARF) and Basmati (BRF) varieties, and the black rice Negro (NRF) variety, were investigated in this study in terms of proximate composition, solvent retention capacity (SRC), and thermo-mechanical properties of dough and bread quality. The flours fell into the classes with very low (NRF) and low amylose content (ARF and BRF); NRF flour had the highest contents of protein (11.16%), crude fiber (4.75%), ash (1.30%), and fats (3.42%). Correlations were obtained between CaCl2-SRC and fiber content, and between NaCl-SRC and lactic acid-SRC and protein content. The dough prepared from BRF had a lower value of the thermo-mechanical weakening, which suggests a higher resistance of the proteins to kneading. On the other hand, NRF exhibited a higher value of thermo-mechanical weakening, suggesting a lower protein resistance. Increasing the amount of water used to prepare the dough resulted in the decrease of the rate of starch retrogradation, while the breakdown decreased for BRF and remained constant for ARF and NRF. The specific volume and texture of the bread have been improved by using a larger amount of water in the preparation of the dough. The texture was negatively correlated (p < 0.05) with the specific volume of the bread. The best quality bread was obtained when the NRF was used.
Multi-Scale Approach of HCF Taking into Account Plasticity and Damage: Application to LPBF Materials
Authors: Koutiri Imade , Andreau Olivier , Peyre Patrice
Published: April 2022

Laser additive manufacturing enables economical production of complex lightweight structures. To realize the potential benefits of additive manufacturing technology in industrial applications, the fatigue performance of parts additively manufactured materials must be modelized. The aim of this paper is to present a new modeling approach combining plasticity and damage, and appropriate for as-built Laser-Powder Bed Fusion (LPBF) structures. The model presented is an extension of the Dang Van criterion, including damage, defined as porosity in the case of LPBF. Attention is focused on the integration of damage in a fatigue criterion using the concept of elastic shakedown. Finally, the case of 316L will illustrate the results of the model by fatigue tests with deterministic defects.
The Impact of Improved Topographic Resolution on the Distribution of Terrain Spectra and Grid-Size Selection for Mesoscale Models
Authors: Wang Chengxin , Liang Li , Zhang Wancheng , Gao Shouting , Yang Shuai
Published: April 2022

Spectral analysis of terrain height variance is conducive to quantitatively study the terrain characteristics and grid-size selection for mesoscale models. Improved topographic resolutions can lead to the variations of terrain characteristics and the appropriate grid size for a fixed analyzed area. Spectral methods of one-dimensional weighted average and arithmetic mean were used to investigate the specific impact on the distribution of terrain spectra and grid-size selection for mesoscale models of the landslide-prone areas in western Sichuan. The results indicate that the maximum spectral energy (the variance of the terrain height series) of 30″ resolution (R1) is larger than that of 90 m resolution (R2), indicating a gentler undulation of terrain for R2. The spectral curve of R2 almost overlaps with that of R1 because the difference in topographic resolution does not change the dominant distribution of the topography. Their differing spectral energies at longer wavelengths are related to the majority of grid points of R2 distributed at shorter wavelength bands. A least squares fit in the form of S=aλb was used to estimate the decreasing trend of the spectral distribution. The difference in spectral slope between R1 and R2 is mainly caused by the spectral energy of R2 at shorter wavelengths. The exponent b is connected with grid-size selection for mesoscale models. A universal horizontal grid size of 2.5 km for R1 and 1.9 km for R2 are required to resolve 95% of the terrain height variance for a mesoscale model application without a subgrid-scale parameterization. The simulation tests show that the improved topographic resolution appears to perform better in reproducing precipitation, which is probably related to the finer details of the terrain recognized by the model.
Density Estimation of Fog in Image Based on Dark Channel Prior
Authors: Guo Hong , Wang Xiaochun , Li Hongjun
Published: April 2022

This paper proposes a method and an original index for the estimation of fog density using images or videos. The proposed method had the advantages of convenient operation and low costs for applications in automatic driving and environmental monitoring. The index was constructed based on a dark channel map and the pseudo-edge details of the foggy image. The effectiveness of the fog density index was demonstrated and validated through experiments on the two existing open datasets. The experimental results showed that the presented index could correctly estimate the fog density of images: (1) the estimated fog density value was consistent with the corresponding label in the Color Hazy Image Database (CHIC) in terms of rank order; (2) the estimated fog density level was consistent with the corresponding label in the Cityscapes database and the accuracy reached as high as 0.9812; (3) the proposed index could be used to evaluate the performance of a video defogging algorithm in terms of residual fog.
The Classification of All Singular Nonsymmetric Macdonald Polynomials
Authors: Dunkl Charles
Published: April 2022

The affine Hecke algebra of type A has two parameters q,t and acts on polynomials in N variables. There are two important pairwise commuting sets of elements in the algebra: the Cherednik operators and the Jucys–Murphy elements whose simultaneous eigenfunctions are the nonsymmetric Macdonald polynomials, and basis vectors of irreducible modules of the Hecke algebra, respectively. For certain parameter values, it is possible for special polynomials to be simultaneous eigenfunctions with equal corresponding eigenvalues of both sets of operators. These are called singular polynomials. The possible parameter values are of the form qm=t−n with 2≤n≤N. For a fixed parameter, the singular polynomials span an irreducible module of the Hecke algebra. Colmenarejo and the author (SIGMA 16 (2020), 010) showed that there exist singular polynomials for each of these parameter values, they coincide with specializations of nonsymmetric Macdonald polynomials, and the isotype (a partition of N) of the Hecke algebra module is dn−1,n−1,…,n−1,r for some d≥1. In the present paper, it is shown that there are no other singular polynomials.
In Vivo Model of Osteoarthritis to Compare Allogenic Amniotic Epithelial Stem Cells and Autologous Adipose Derived Cells
Authors: Veronesi Francesca , Fini Milena , Martini Lucia , Berardinelli Paolo , Russo Valentina , Filardo Giuseppe , Di Matteo Berardo , Marcacci Maurilio , Kon Elizaveta
Published: April 2022

The challenge of osteoarthritis (OA) is to find a minimally invasive orthobiological therapy to contrast OA progression, on inflammatory and structural fronts. The aim of the present study is to compare the effects of an intra-articular injection of three orthobiological treatments, autologous culture expanded adipose-derived mesenchymal stromal cells (ADSCs), autologous stromal vascular fraction (SVF) and allogenic culture expanded amniotic epithelial stem cells (AECs), in an animal model of OA. OA was induced in 24 sheep by bilateral lateral meniscectomy and, at 3 and 6 months post-treatment, the results were analyzed with macroscopy, histology, histomorphometry, and biochemistry. All the three treatments showed better results than control (injection of NaCl), but SVF and AECs showed superiority over ADSCs, because they induced higher cartilage regeneration and lower inflammation. SVF showed better results than AECs at 3 and 6 months. To conclude, SVF seems to be more favorable than the other biological options, because it is easily obtained and rapidly used after harvesting, with good healing potential. AECs cause no discomfort and could be also considered for the treatment of OA joints.
Neuregulin (NRG-1β) Is Pro-Myogenic and Anti-Cachectic in Respiratory Muscles of Post-Myocardial Infarcted Swine
Authors: Galindo Cristi L. , Nguyen Van Thuan , Hill Braxton , Easterday Ethan , Cleator John H. , Sawyer Douglas B.
Published: April 2022

Neuregulin-1β (NRG-1β) is a growth and differentiation factor with pleiotropic systemic effects. Because NRG-1β has therapeutic potential for heart failure and has known growth effects in skeletal muscle, we hypothesized that it might affect heart failure-associated cachexia, a severe co-morbidity characterized by a loss of muscle mass. We therefore assessed NRG-1β’s effect on intercostal skeletal muscle gene expression in a swine model of heart failure using recombinant glial growth factor 2 (USAN-cimaglermin alfa), a version of NRG-1β that has been tested in humans with systolic heart failure. Animals received one of two intravenous doses (0.67 or 2 mg/kg) of NRG-1β bi-weekly for 4 weeks, beginning one week after infarct. Based on paired-end RNA sequencing, NRG-1β treatment altered the intercostal muscle gene expression of 581 transcripts, including genes required for myofiber growth, maintenance and survival, such as MYH3, MYHC, MYL6B, KY and HES1. Importantly, NRG-1β altered the directionality of at least 85 genes associated with cachexia, including myostatin, which negatively regulates myoblast differentiation by down-regulating MyoD expression. Consistent with this, MyoD was increased in NRG-1β-treated animals. In vitro experiments with myoblast cell lines confirmed that NRG-1β induces ERBB-dependent differentiation. These findings suggest a NRG-1β-mediated anti-atrophic, anti-cachexia effect that may provide additional benefits to this potential therapy in heart failure.
agReg-SNPdb-Plants: A Database of Regulatory SNPs for Agricultural Plant Species
Authors: Klees Selina , Heinrich Felix , Schmitt Armin Otto , Gültas Mehmet
Published: April 2022

Single nucleotide polymorphisms (SNPs) that are located in the promoter regions of genes and affect the binding of transcription factors (TFs) are called regulatory SNPs (rSNPs). Their identification can be highly valuable for the interpretation of genome-wide association studies (GWAS), since rSNPs can reveal the biologically causative variant and decipher the regulatory mechanisms behind a phenotype. In our previous work, we presented agReg-SNPdb, a database of regulatory SNPs for agriculturally important animal species. To complement this previous work, in this study we present the extension agReg-SNPdb-Plants storing rSNPs and their predicted effects on TF-binding for 13 agriculturally important plant species and subspecies (Brassica napus, Helianthus annuus, Hordeum vulgare, Oryza glaberrima, Oryza glumipatula, Oryza sativa Indica, Oryza sativa Japonica, Solanum lycopersicum, Sorghum bicolor, Triticum aestivum, Triticum turgidum, Vitis vinifera, and Zea mays). agReg-SNPdb-Plants can be queried via a web interface that allows users to search for SNP IDs, chromosomal regions, or genes. For a comprehensive interpretation of GWAS results or larger SNP-sets, it is possible to download the whole list of SNPs and their impact on transcription factor binding sites (TFBSs) from the website chromosome-wise.