bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of menstrual cycle on hemodynamic and autonomic responses to central hypovolemia
Authors: Shankhwar Vishwajeet , Urvec Janez , Steuber Bianca , Schmid Zalaudek Karin , Saloň Adam , Hawliczek Anna , Bergauer Andrej , Aljasmi Khawla , Abdi Asrar , Naser Asmaa , Himeidi Maya , Alsuwaidi Hanan , Du Plessis Stefan , Alsheikh-Ali Alawi , Kellett Catherine , Bayoumi Riad , Blaber Andrew Phillip , Goswami Nandu
Published: February 2024

Background

Estrogen and progesterone levels undergo changes throughout the menstrual cycle. Existing literature regarding the effect of menstrual phases on cardiovascular and autonomic regulation during central hypovolemia is contradictory.

Aims and study

This study aims to explore the influence of menstrual phases on cardiovascular and autonomic responses in both resting and during the central hypovolemia induced by lower body negative pressure (LBNP). This is a companion paper, in which data across the menstrual phases from healthy young females, whose results are reported in Shankwar et al. (2023), were further analysed.

Methods

The study protocol consisted of three phases: (1) 30 min of supine rest; (2) 16 min of four LBNP levels; and (3) 5 min of supine recovery. Hemodynamic and autonomic responses (assessed via heart rate variability, HRV) were measured before-, during-, and after-LBNP application using Task Force Monitor® (CNSystems, Graz, Austria). Blood was also collected to measure estrogen and progesterone levels.

Results

In this companion paper, we have exclusively assessed 14 females from the previous study (Shankwar et al., 2023): 8 in the follicular phase of the menstrual cycle (mean age 23.38 ± 3.58 years, height 166.00 ± 5.78 cm, weight 57.63 ± 5.39 kg and BMI of 20.92 ± 1.96 25 kg/m2) and 6 in the luteal phase (mean age 22.17 ± 1.33 years, height 169.83 ± 5.53 cm, weight 62.00 ± 7.54 kg and BMI of 21.45 ± 2.63 kg/m2). Baseline estrogen levels were significantly different from the follicular phase as compared to the luteal phase: (33.59 pg/ml, 108.02 pg/ml, respectively, p < 0.01). Resting hemodynamic variables showed no difference across the menstrual phases. However, females in the follicular phase showed significantly lower resting values of low-frequency (LF) band power (41.38 ± 11.75 n.u. and 58.47 ± 14.37 n.u., p = 0.01), but higher resting values of high frequency (HF) band power (58.62 ± 11.75 n.u. and 41.53 ± 14.37 n.u., p = 0.01), as compared to females in the luteal phase. During hypovolemia, the LF and HF band powers changed only in the follicular phase F(1, 7) = 77.34, p < 0.0001 and F(1, 7) = 520.06, p < 0.0001, respectively.

Conclusions

The menstrual phase had an influence on resting autonomic variables, with higher sympathetic activity being observed during the luteal phase. Central hypovolemia leads to increased cardiovascular and autonomic responses, particularly during the luteal phase of the menstrual cycle, likely due to higher estrogen levels and increased sympathetic activity.
Asymmetric growth-limiting development of the female conceptus
Authors: Estrella Consuelo Amor S. , Gatford Kathryn L. , Xiang Ruidong , Javadmanesh Ali , Ghanipoor-Samami Mani , Nattrass Greg S. , Shuaib Entesar , McAllister Milton M. , Beckman Ian , Thomsen Dana A. , Clifton Vicki L. , Owens Julie A. , Roberts Claire T. , Hiendleder Stefan , Kind Karen L.
Published: February 2024

Introduction

Sex differences in prenatal growth may contribute to sex-dependent programming effects on postnatal phenotype.

Methods

We integrated for the first time phenotypic, histomorphological, clinico-chemical, endocrine and gene expression analyses in a single species, the bovine conceptus at mid-gestation.

Results

We demonstrate that by mid-gestation, before the onset of accelerated growth, the female conceptus displays asymmetric lower growth compared to males. Female fetuses were smaller with lower ponderal index and organ weights than males. However, their brain:body weight, brain:liver weight and heart:body weight ratios were higher than in males, indicating brain and heart ‘sparing’. The female placenta weighed less and had lower volumes of trophoblast and fetal connective tissue than the male placenta. Female umbilical cord vessel diameters were smaller, and female-specific relationships of body weight and brain:liver weight ratios with cord vessel diameters indicated that the umbilico-placental vascular system creates a growth-limiting environment where blood flow is redistributed to protect brain and heart growth. Clinico-chemical indicators of liver perfusion support this female-specific growth-limiting phenotype, while lower insulin-like growth factor 2 (IGF2) gene expression in brain and heart, and lower circulating IGF2, implicate female-specific modulation of key endocrine mediators by nutrient supply.

Conclusion

This mode of female development may increase resilience to environmental perturbations in utero and contribute to sex-bias in programming outcomes including susceptibility to non-communicable diseases.
Efficacy of pasireotide LAR for acromegaly: a prolonged real-world monocentric study
Authors: Favero Vittoria , Zampetti Benedetta , Carioni Emanuela I. , Dalino Ciaramella Paolo , Grossrubatscher Erika , Dallabonzana Daniela , Chiodini Iacopo , Cozzi Renato
Published: February 2024

Background

Acromegaly is caused by excessive growth hormone (GH) and insulin-like growth factor 1 (IGF1). Medical therapy plays a role as a treatment option for persistent disease after non-curative surgery or as a first-line therapy when surgery is not feasible. Pasireotide-LAR (Pas-LAR) is recommended for patients with acromegaly as second-line treatment.

Aim

To evaluate the patients characteristics predictive of an adequate response to Pas-LAR and the long-term efficacy and safety of the Pas-LAR treatment.

Methods

Data from 19 patients with active acromegaly, who were and resistant or intolerant to first-line medical therapy and were switched to pas-LAR have been retrospectively collected. We compared the baseline clinical and biochemical characteristics of patients who were found to respond to Pas-LAR therapy (responders, n=14) with those of patients who did not respond (non-responders, n=5). We then evaluated the Pas-LAR efficacy and safety during long-term follow-up in responders.

Results

IGF1 normalization occurred in 71.4% of responders after one injection. IGF1 levels, [median(interquartile range) of the upper limit of the normal range (ULN) fold increase] were higher in non-responders compared to responders within the initial month of therapy [1.40(1.30-2.34) vs 0.70(0.55-1.25), respectively, p=0.009] and after three [1.77(1.74-2.29) vs 0.94(0.82-1.13), respectively, p=0.029] and six months [1.68(1.33-1.72) vs 1.00(0.65 -1.28), respectively, p=0.002]. Out of 6 patients with symptomatic headache (all in responder group), 5 and 1 reported the resolution and improvement of headache, respectively, already after the first injection. Median HbA1c levels tended to increase from baseline to 6 months both in responder (36 mMol/Mol to 42 mMol/Mol) and non-responder patients (45 mMol/Mol to 48 mMol/Mol). During long term follow up, in the responder group 2 new patients developed diabetes. Tumor shrinkage was observed in 6 out of 7 evaluated responders, with no cases of size increase during the long-term follow-up.

Conclusion

Pas-LAR is effective and safe and the early identification of responders is possible just after the first administration.
A pathway to 25% reduction in greenhouse gas emission by 2030 using GIS-Based multi-criteria decision making for renewables, date palm (phoenix dactylifera), and carbon credits: a case for Qatar
Authors: Bilal Hazrat , Okonkwo Eric C. , Al-Ansari Tareq
Published: February 2024

Qatar is making concerted efforts at the national level to decrease anthropogenic greenhouse gas emissions. The country has set its National Determined Contribution (NDC) in line with the Paris Agreement to reduce its overall emissions by 25% by the year 2030. This study identifies several pathways, which Qatar can explore to achieve its set goals. It explores Qatar’s potential for renewable energy generation (solar and wind), carbon offset through planting trees, and the purchase of carbon offset credits. The study starts by investigating the available wind and solar energy potential in the country, by considering physical and legal restrictions, which limit the available land to be utilized for RE generation. Multi-criteria Decision is carried out utilizing the Analytic Hierarchy Process (AHP) for variable percentage estimation in ArcMap Geographic Information Systems software. Next, a review based on Qatar’s climate is performed to identify suitable tree species that would promote atmospheric CO2 sequestration in the country. Finally, the cost implication of offsetting emissions using verified carbon credits is considered and compared with other options. Results indicated that while a considerable area is available for both wind (37%) and solar (53%) energy generation, only 6.2% and 13.8% of Qatar are highly suitable for wind and solar energy installations, respectively. Also, while Qatar has the potential to meet 20% of its energy demand by 2030 from renewable sources by using solar PV and wind, this will only account for a 14.28% reduction in overall emissions. Additionally, planting date palm trees (Phoenix dactylifera) would significantly benefit the nation in the area of carbon sequestration and revenue generation as compared to purchasing carbon offset credits.
A new-fangled connection of UPQC tailored power device from wind farm to weak-grid
Authors: Pushkarna Mukesh , Govardhan Rao Kambhampati Venkata , Goud B. Srikanth , Kumar M. Kiran , Reddy Ch. Rami , Kotb Hossam , AboRas Kareem M. , Alharthi Yahya Z. , Yousef Amr
Published: February 2024

A significant portion of wind power conversion systems worldwide comprise wind farms (WFs) that use Squirrel Cage Induction Generator (SCIG) and are directly linked to the power grid. In facilities that generate electrical energy at a moderate level, WFs are connected by means of distribution systems that use medium voltage (MV). It is not uncommon for such a system to produce a scenario in which the amount of electricity generated corresponds to the grid’s transit volume. When a wind farm’s wind power generation system is connected to a weak grid, the lack of potential control of the Point of Common Coupling (PCC) is a primary issue. This strategy is called a “Wind Farm with Weak Grid Connection.” Therefore, the amalgamation of weak grids, fluctuating electricity from wind, and variations in load on the system cause disruptions in the PCC voltage, further degrading the Power Quality (PQ) and the WF stability. Either the control method at the production level or the compensating strategies at the PCC level can improve this situation. If wind farms are built on SCIG and are directly linked to the grid, it is essential to utilise the last substitute. The technology known as Custom Power Devices (CUPS), proved extremely helpful for this type of application. This study presents a compensation technique based on a specific CUPS device, known as the Unified Power Quality Compensator (UPQC), as a possible solution. The potential terminals of WF needed to be regulated, and the voltage fluctuations on the grid side required to be reduced, so a custom-made control strategy for the UPQC device was designed internally. The control of power, such as active and reactive in the UPQC’s series and shunt converters, as well as the transmission of power via the UPQC DC-Link between converters, are the foundation of the internal control strategy that has been developed. Compared to other bespoke tactics that use reactive power, this strategy increases the UPQC’s capability to provide compensation. The suggested study calculates THD using a FUZZY controller. The results are compared to PI controller results. Simulation findings show how the suggested compensating strategy can minimise THD values and improve wind farm power and stability. The simulations suggest that the proposed compensating strategy enhances WF power and stability.
Heavy metal accumulation and health risk assessment in S. alterniflora Loisel. and native plant Suaeda salsa (L.) Pall. in Dongtai Tiaozini wetland
Authors: Li Guo , Cai Zhenjie , Huang Suzhen , Song Zijie , Zhang Yumiao , Zheng Zheng , Luo Ruixue , Wang Shoubing
Published: February 2024

Coastal wetlands play an irreplaceable role in the global ecosystem, and both human activities and natural factors may lead to the contamination of Tiaozini coastal wetland with heavy metals. The study was conducted to determine the contents of eight heavy metals, Hg, Cd, Cr, As, Pb, Cu, Ni, and Zn, in the above-ground and below-ground parts of the plants and in the rhizosphere sediment, using the invasive species S. alterniflora and the native plant S. glauca, calculating the geoaccumulation index (Igeo), bioaccumulation factor, transfer factor, total target risk quotient (TTHQ), and carcinogenicity risk (CR), to analyze the transfer characteristics and potential health risks to human beings of the heavy metals in plants. This study aims to investigate the enrichment characteristics of the dominant plant, S. alterniflora Loisel. (S. alterniflora) and Suaeda salsa (L.) Pall. (S. glauca). Regarding heavy metals, eight common heavy metal elements were selected, including Hg, Cd, Cr, As, Pb, Cu, Ni, and Zn, and examined their content in surface sediments and different parts of the two plants. The transfer characteristics of heavy metals in the plant body and their potential health risks to humans were also analyzed. These findings suggest that both plants accumulate higher concentrations of heavy metals in their below-ground parts. Cr, Cu, and Zn had the highest average concentrations in both plants. Geoaccumulation index (Igeo) indicated that the Tiaozini Wetland is not yet contaminated. S. alterniflora had transfer factors less than 1 for all heavy metals, while S. glauca had transfer factors greater than 1. Both plants had a certain purifying effect on heavy metal pollution in wetlands, including Cr, Cd, Cu, and Zn. However, Cr and As in the below-ground part of S. alterniflora and Cr in the above-ground part of S. glauca had a target hazard quotient (THQ) greater than 1, indicating a potential health risk to humans, but the carcinogenic risk is low. For other heavy metals, THQ was less than 1, indicating no health risk. The total target hazard quotient (TTHQ) of different parts of both plants was greater than 1, which must be taken into account when considering their suitability as edible resources.
Interactions between CNS and immune cells in tuberculous meningitis
Authors: Ma Quan , Chen Jinyun , Kong Xingxing , Zeng Yuqin , Chen Zhanpeng , Liu Huazhen , Liu Lanlan , Lu Shuihua , Wang Xiaomin
Published: February 2024

The central nervous system (CNS) harbors its own special immune system composed of microglia in the parenchyma, CNS-associated macrophages (CAMs), dendritic cells, monocytes, and the barrier systems within the brain. Recently, advances in the immune cells in the CNS provided new insights to understand the development of tuberculous meningitis (TBM), which is the predominant form of Mycobacterium tuberculosis (M.tb) infection in the CNS and accompanied with high mortality and disability. The development of the CNS requires the protection of immune cells, including macrophages and microglia, during embryogenesis to ensure the accurate development of the CNS and immune response following pathogenic invasion. In this review, we summarize the current understanding on the CNS immune cells during the initiation and development of the TBM. We also explore the interactions of immune cells with the CNS in TBM. In the future, the combination of modern techniques should be applied to explore the role of immune cells of CNS in TBM.
Specificities and redundancies in the NEL family of bacterial E3 ubiquitin ligases of Salmonella enterica serovar Typhimurium
Authors: Bullones-Bolaños Andrea , Martín-Muñoz Paula , Vallejo-Grijalba Claudia , Bernal-Bayard Joaquín , Ramos-Morales Francisco
Published: February 2024

Salmonella enterica serovar Typhimurium expresses two type III secretion systems, T3SS1 and T3SS2, which are encoded in Salmonella pathogenicity island 1 (SPI1) and SPI2, respectively. These are essential virulent factors that secrete more than 40 effectors that are translocated into host animal cells. This study focuses on three of these effectors, SlrP, SspH1, and SspH2, which are members of the NEL family of E3 ubiquitin ligases. We compared their expression, regulation, and translocation patterns, their role in cell invasion and intracellular proliferation, their ability to interact and ubiquitinate specific host partners, and their effect on cytokine secretion. We found that transcription of the three genes encoding these effectors depends on the virulence regulator PhoP. Although the three effectors have the potential to be secreted through T3SS1 and T3SS2, the secretion of SspH1 and SspH2 is largely restricted to T3SS2 due to their expression pattern. We detected a role for these effectors in proliferation inside fibroblasts that is masked by redundancy. The generation of chimeric proteins allowed us to demonstrate that the N-terminal part of these proteins, containing the leucine-rich repeat motifs, confers specificity towards ubiquitination targets. Furthermore, the polyubiquitination patterns generated were different for each effector, with Lys48 linkages being predominant for SspH1 and SspH2. Finally, our experiments support an anti-inflammatory role for SspH1 and SspH2.
Fraction of exhaled nitric oxide is higher in liver transplant recipients than in controls from the general population: a cohort study
Authors: Arentoft Nicoline S. , Fialla Annette D. , Krohn Paul S. , Patursson Magda T. , Thudium Rebekka F. , Suarez-Zdunek Moises A. , Høgh Julie , Lauridsen Emilie H. E. , Hansen Jesper B. , Jensen Jens-Ulrik S. , Perch Michael , Møller Dina L. , Pommergaard Hans-Christian , Aagaard Niels K. , Davidsen Jesper R. , Lange Peter , Çolak Yunus , Afzal Shoaib , Nordestgaard Børge G. , Rasmussen Allan , Nielsen Susanne D.
Published: February 2024

Background

Fraction of exhaled nitric oxide with an expiratory flow of 50 mL/s (FENO50) is a biomarker of eosinophilic airway inflammation. Liver transplant recipients have an increased risk of pulmonary infections, but little is known about the burden of chronic pulmonary diseases in this group. We aimed to assess the prevalence of elevated FENO50 in liver transplant recipients and compare it to controls from the general population.

Methods

FENO50 was measured in 271 liver transplant recipients from The Danish Comorbidity in Liver Transplant Recipients (DACOLT) study and 1,018 age- and sex-matched controls from The Copenhagen General Population Study (CGPS). Elevated FENO50 was defined as ≥25 or ≥50 parts per billion (ppb). The analyses were adjusted for known and suspected confounders.

Results

The median age of the liver transplant recipients was 55 years (interquartile range (IQR) 46–64), and 58% were men. The liver transplant recipients had a higher median FENO50 than the controls [16 ppb (IQR 10–26) vs. 13 ppb (IQR 8–18.), p < 0.001]. Furthermore, the liver transplant recipients had a higher prevalence of elevated FENO50 (for FENO50 ≥25 ppb 27% vs. 11%, p < 0.001 and ≥50 ppb 4% vs. 2%, p = 0.02). The results were similar after adjusting for age, sex, smoking status, use of airway medication, and blood eosinophil counts [the adjusted odds ratio (OR) for FENO50 ≥25 ppb was 3.58 (95% CI: 2.50–5.15, p < 0.0001) and the adjusted OR for FENO50 ≥50 ppb was 3.14 (95% CI: 1.37–7.20, p = 0.007)].

Conclusion

The liver transplant recipients had elevated FENO50, implying increased eosinophilic airway inflammation. The clinical impact of this finding needs further investigation.
Gut microbiota and dietary intervention: affecting immunotherapy efficacy in non–small cell lung cancer
Authors: Xin Yu , Liu Chen-Guang , Zang Dan , Chen Jun
Published: February 2024

Non–small cell lung cancer (NSCLC) accounts for 80–85% of all lung cancers. In recent years, treatment with immune checkpoint inhibitors (ICIs) has gradually improved the survival rate of patients with NSCLC, especially those in the advanced stages. ICIs can block the tolerance pathways that are overexpressed by tumor cells and maintain the protective activity of immune system components against cancer cells. Emerging clinical evidence suggests that gut microbiota may modulate responses to ICIs treatment, possibly holding a key role in tumor immune surveillance and the efficacy of ICIs. Studies have also shown that diet can influence the abundance of gut microbiota in humans, therefore, dietary interventions and the adjustment of the gut microbiota is a novel and promising treatment strategy for adjunctive cancer therapy. This review comprehensively summarizes the effects of gut microbiota, antibiotics (ATBs), and dietary intervention on the efficacy of immunotherapy in NSCLC, with the aim of informing the development of novel strategies in NSCLC immunotherapy.
Tumor epitope spreading by a novel multivalent therapeutic cellular vaccine targeting cancer antigens to invariant NKT-triggered dendritic cells in situ
Authors: Yamasaki Satoru , Shimizu Kanako , Fujii Shin-ichiro
Published: February 2024

Introduction

Cancer is categorized into two types based on the microenvironment: cold and hot tumors. The former is challenging to stimulate through immunity. The immunogenicity of cancer relies on the quality and quantity of cancer antigens, whether recognized by T cells or not. Successful cancer immunotherapy hinges on the cancer cell type, antigenicity and subsequent immune reactions. The T cell response is particularly crucial for secondary epitope spreading, although the factors affecting these mechanisms remain unknown. Prostate cancer often becomes resistant to standard therapy despite identifying several antigens, placing it among immunologically cold tumors. We aim to leverage prostate cancer antigens to investigate the potential induction of epitope spreading in cold tumors. This study specifically focuses on identifying factors involved in secondary epitope spreading based on artificial adjuvant vector cell (aAVC) therapy, a method established as invariant natural killer T (iNKT) -licensed DC therapy.

Methods

We concentrated on three prostate cancer antigens (prostate-specific membrane antigen (PSMA), prostate-specific antigen (PSA), and prostatic acid phosphatase (PAP)). By introducing allogeneic cells with the antigen and murine CD1d mRNA, followed by α-galactosylceramide (α-GalCer) loading, we generated five types of aAVCs, i.e, monovalent, divalent and trivalent antigen-expressing aAVCs and four types of prostate antigen-expressing cold tumors. We evaluated iNKT activation and antigen-specific CD8+ T cell responses against tumor cells prompted by the aAVCs.

Results

Our study revealed that monovalent aAVCs, expressing a single prostate antigen, primed T cells for primary tumor antigens and also induced T cells targeting additional tumor antigens by triggering a tumor antigen-spreading response. When we investigated the immune response by trivalent aAVC (aAVC-PROS), aAVC-PROS therapy elicited multiple antigen-specific CD8+ T cells simultaneously. These CD8+ T cells exhibited both preventive and therapeutic effects against tumor progression.

Conclusions

The findings from this study highlight the promising role of tumor antigen-expressing aAVCs, in inducing efficient epitope spreading and generating robust immune responses against cancer. Our results also propose that multivalent antigen-expressing aAVCs present a promising therapeutic option and could be a more comprehensive therapy for treating cold tumors like prostate cancer.
Involvement and repair of epithelial barrier dysfunction in allergic diseases
Authors: Lu Hui-Fei , Zhou Yi-Chi , Yang Li-Tao , Zhou Qian , Wang Xi-Jia , Qiu Shu-Qi , Cheng Bao-Hui , Zeng Xian-Hai
Published: February 2024

The epithelial barrier serves as a critical defense mechanism separating the human body from the external environment, fulfilling both physical and immune functions. This barrier plays a pivotal role in shielding the body from environmental risk factors such as allergens, pathogens, and pollutants. However, since the 19th century, the escalating threats posed by environmental pollution, global warming, heightened usage of industrial chemical products, and alterations in biodiversity have contributed to a noteworthy surge in allergic disease incidences. Notably, allergic diseases frequently exhibit dysfunction in the epithelial barrier. The proposed epithelial barrier hypothesis introduces a novel avenue for the prevention and treatment of allergic diseases. Despite increased attention to the role of barrier dysfunction in allergic disease development, numerous questions persist regarding the mechanisms underlying the disruption of normal barrier function. Consequently, this review aims to provide a comprehensive overview of the epithelial barrier’s role in allergic diseases, encompassing influencing factors, assessment techniques, and repair methodologies. By doing so, it seeks to present innovative strategies for the prevention and treatment of allergic diseases.
Sex-specific outcomes in cancer therapy: the central role of hormones
Authors: Bakhshi Parisa , Ho Jim Q. , Zanganeh Steven
Published: February 2024

Sex hormones play a pivotal role in modulating various physiological processes, with emerging evidence underscoring their influence on cancer progression and treatment outcomes. This review delves into the intricate relationship between sex hormones and cancer, elucidating the underlying biological mechanisms and their clinical implications. We explore the multifaceted roles of estrogen, androgens, and progesterone, highlighting their respective influence on specific cancers such as breast, ovarian, endometrial, and prostate. Special attention is given to estrogen receptor-positive (ER+) and estrogen receptor-negative (ER−) tumors, androgen receptor signaling, and the dual role of progesterone in both promoting and inhibiting cancer progression. Clinical observations reveal varied treatment responses contingent upon hormonal levels, with certain therapies like tamoxifen, aromatase inhibitors, and anti-androgens demonstrating notable success. However, disparities in treatment outcomes between males and females in hormone-sensitive cancers necessitate further exploration. Therapeutically, the utilization of hormone replacement therapy (HRT) during cancer treatments presents both potential risks and benefits. The promise of personalized therapies, tailored to an individual’s hormonal profile, offers a novel approach to optimizing therapeutic outcomes. Concurrently, the burgeoning exploration of new drugs and interventions targeting hormonal pathways heralds a future of more effective and precise treatments for hormone-sensitive cancers. This review underscores the pressing need for a deeper understanding of sex hormones in cancer therapy and the ensuing implications for future therapeutic innovations.
Association of structural connectivity with functional brain network segregation in a middle-aged to elderly population
Authors: Schulz Maximilian , Petersen Marvin , Cheng Bastian , Thomalla Götz
Published: February 2024

Introduction

The deterioration of white matter pathways is one of the hallmarks of the ageing brain. In theory, this decrease in structural integrity leads to disconnection between regions of brain networks and thus to altered functional connectivity and a decrease in cognitive abilities. However, in many studies, associations between structural and functional connectivity are rather weak or not observed at all. System segregation, defined as the extent of partitioning between different resting state networks has increasingly gained attention in recent years as a new metric for functional changes in the aging brain. Yet there is a shortage of previous reports describing the association of structural integrity and functional segregation.

Methods

Therefore, we used a large a large sample of 2,657 participants from the Hamburg City Health Study, a prospective population-based study including participants aged 46–78 years from the metropolitan region Hamburg, Germany. We reconstructed structural and functional connectomes to analyze whether there is an association between age-related differences in structural connectivity and functional segregation, and whether this association is stronger than between structural connectivity and functional connectivity. In a second step, we investigated the relationship between functional segregation and executive cognitive function and tested whether this association is stronger than that between functional connectivity and executive cognitive function.

Results

We found a significant age-independent association between decreasing structural connectivity and decreasing functional segregation across the brain. In addition, decreasing functional segregation showed an association with decreasing executive cognitive function. On the contrary, no such association was observed between functional connectivity and structural connectivity or executive function.

Discussion

These results indicate that the segregation metric is a more sensitive biomarker of cognitive ageing than functional connectivity at the global level and offers a unique and more complementary network-based explanation.
YOLOv8-ACU: improved YOLOv8-pose for facial acupoint detection
Authors: Yuan Zijian , Shao Pengwei , Li Jinran , Wang Yinuo , Zhu Zixuan , Qiu Weijie , Chen Buqun , Tang Yan , Han Aiqing
Published: February 2024

Introduction

Acupoint localization is integral to Traditional Chinese Medicine (TCM) acupuncture diagnosis and treatment. Employing intelligent detection models for recognizing facial acupoints can substantially enhance localization accuracy.

Methods

This study introduces an advancement in the YOLOv8-pose keypoint detection algorithm, tailored for facial acupoints, and named YOLOv8-ACU. This model enhances acupoint feature extraction by integrating ECA attention, replaces the original neck module with a lighter Slim-neck module, and improves the loss function for GIoU.

Results

The YOLOv8-ACU model achieves impressive accuracy, with an mAP@0.5 of 97.5% and an mAP@0.5–0.95 of 76.9% on our self-constructed datasets. It also marks a reduction in model parameters by 0.44M, model size by 0.82 MB, and GFLOPs by 9.3%.

Discussion

With its enhanced recognition accuracy and efficiency, along with good generalization ability, YOLOv8-ACU provides significant reference value for facial acupoint localization and detection. This is particularly beneficial for Chinese medicine practitioners engaged in facial acupoint research and intelligent detection.
MR-guided focused ultrasound thalamotomy for lithium-induced tremor: a case report and literature review
Authors: Gelman Kate , Melott Joseph , Thakur Vishal , Tarabishy Abdul R. , Brandt Ana , Konrad Peter , Ranjan Manish , Memon Adeel A.
Published: February 2024

Drug-induced tremor is a common side effect of lithium with an occurrence of approximately 25% of patients. Cessation of the offending drug can be difficult, and many medical treatments for drug-induced tremor are ineffective. Deep brain stimulation (DBS) has been shown in a limited number of case reports to effectively reduce drug-induced tremor, however, which remains an invasive therapeutic option. MR-guided focused ultrasound (MRgFUS) thalamotomy is an FDA-approved non-invasive treatment for essential tremor (ET). To the best of our knowledge, MRgFUS thalamotomy has never been reported to treat drug-induced tremor. Here, we present a case of a left-handed 55-year-old man with a progressive, medically refractory lithium-induced tremor of the bilateral upper extremities. The patient underwent MRgFUS thalamotomy targeting the right ventral intermediate nucleus (VIM) of the thalamus to treat the left hand. There was almost complete resolution of his left-hand tremor immediately following MRgFUS. There were no side effects. The patient continues to show excellent tremor control at 90-day follow-up and remains free from side effects. This case demonstrates MRgFUS thalamotomy as a possible novel treatment option to treat drug-induced tremor.
Pharmacokinetics and pharmacodynamics across infusion rates of intravenously administered nipocalimab: results of a phase 1, placebo-controlled study
Authors: Leu Jocelyn H. , Vermeulen An , Abbes Claudia , Arroyo Santiago , Denney William S. , Ling Leona E.
Published: February 2024

Introduction

Nipocalimab is a high-affinity, fully human, aglycosylated, effectorless, immunoglobulin G (IgG) 1 monoclonal antibody that targets the neonatal Fc receptor (FcRn), decreases systemic IgG including autoantibodies, and is under development in several IgG autoantibody- and alloantibody-mediated diseases, including generalized myasthenia gravis, chronic inflammatory demyelinating polyneuropathy, maternal-fetal medicine, and multiple other therapeutic areas. An initial phase 1 study with single and multiple ascending doses of nipocalimab infused intravenously (IV) over 2 h demonstrated dose-dependent serum pharmacokinetics and IgG reductions, with an adverse event (AE) profile comparable to placebo.

Methods

The current investigation evaluates the safety, tolerability, pharmacokinetics, and pharmacodynamics of single doses of nipocalimab across various IV infusion rates in a randomized, double-blind, placebo-controlled, sequential-dose study. Forty participants were randomized to receive nipocalimab 30 mg/kg over 60, 30, 15 or 7.5 min (0.5, 1, 2, or 4 mg/kg/min); nipocalimab 60 mg/kg over 15 min (4 mg/kg/min); or matching placebo.

Results

At doses up to 60 mg/kg and infusion rates up to 4 mg/kg/min (maximum clinically feasible rate), single doses of nipocalimab were tolerable, with 12 (40%) participants experiencing AEs across nipocalimab cohorts compared with 1 (10%) participant in the placebo cohort. AEs deemed treatment related occurred in 6 (20%) participants receiving nipocalimab and 1 (10%) participant receiving placebo. None of the AEs were severe, and no participants discontinued treatment due to AEs. Nipocalimab provided consistent, dose-dependent serum pharmacokinetics and IgG reductions, regardless of infusion rate.

Discussion

This study supports the use of shortened durations of nipocalimab infusion for future studies.
Hepatic perivascular epithelioid cell tumor resembling hepatic adenoma and hepatocellular carcinoma on preoperative imaging: a case report
Authors: Zhu Dongling , Song Shuang , Wang Dongdong , Kuang Dong , Cheng Siyuan , Zhou Jianyuan , Zou Sijuan
Published: February 2024

Perivascular epithelioid cell tumor (PEComa), an uncommon mesenchymal neoplasm, arises from specialized perivascular epithelioid cells exhibiting distinct features of smooth muscle and melanocytic differentiation with unpredictable behavior. PEComa tends to occur more commonly in the uterus and kidneys; its occurrence in the liver is exceedingly rare. We presented a case of a 29-year-old woman with hepatic PEComa and evaluated the tumor with MRI, integrated 18F-fluorodeoxyglucose (FDG), and 68Ga-fibroblast activation protein inhibitor (FAPI) PET/CT scans at presentation. The patient had a history of intermittent utilization of oral contraceptive drugs for several years. An abdominal ultrasound in a physical examination from an outside institution revealed a mass in the liver. A contrast-enhanced abdominal MRI revealed restricted diffusion on diffusion-weighted imaging (DWI) and rapid contrast enhancement and washout patterns in the hepatic lesion, suggesting hepatic adenoma (HA) or hepatocellular carcinoma (HCC). Further assessment was carried out using 18F-FDG and 68Ga-FAPI PET/CT scans. The hepatic lesion was non-FDG avid, whereas increased tracer uptake was observed on the 68Ga-FAPI PET/CT. Subsequently, laparoscopic partial resection of liver segment V was performed. Immunohistochemical analyses demonstrated positive staining for HMB45, Melan-A, and SMA while showing negative results for AFP, glypican-3, hepatocyte, and arginase-1. The results were indicative of a hepatic PEComa diagnosis based on these findings. We also review the current literature on the clinical characteristics, pathological features, and challenges in the diagnosis of hepatic PEComa.
HMGA2 promotes cancer metastasis by regulating epithelial–mesenchymal transition
Authors: Ma Qing , Ye Sisi , Liu Hong , Zhao Yu , Mao Yan , Zhang Wei
Published: February 2024

Epithelial–mesenchymal transition (EMT) is a complex physiological process that transforms polarized epithelial cells into moving mesenchymal cells. Dysfunction of EMT promotes the invasion and metastasis of cancer. The architectural transcription factor high mobility group AT-hook 2 (HMGA2) is highly overexpressed in various types of cancer (e.g., colorectal cancer, liver cancer, breast cancer, uterine leiomyomas) and significantly correlated with poor survival rates. Evidence indicated that HMGA2 overexpression markedly decreased the expression of epithelial marker E-cadherin (CDH1) and increased that of vimentin (VIM), Snail, N-cadherin (CDH2), and zinc finger E-box binding homeobox 1 (ZEB1) by targeting the transforming growth factor beta/SMAD (TGFβ/SMAD), mitogen-activated protein kinase (MAPK), and WNT/beta-catenin (WNT/β-catenin) signaling pathways. Furthermore, a new class of non-coding RNAs (miRNAs, circular RNAs, and long non-coding RNAs) plays an essential role in the process of HMGA2-induced metastasis and invasion of cancer by accelerating the EMT process. In this review, we discuss alterations in the expression of HMGA2 in various types of cancer. Furthermore, we highlight the role of HMGA2-induced EMT in promoting tumor growth, migration, and invasion. More importantly, we discuss extensively the mechanism through which HMGA2 regulates the EMT process and invasion in most cancers, including signaling pathways and the interacting RNA signaling axis. Thus, the elucidation of molecular mechanisms that underlie the effects of HMGA2 on cancer invasion and patient survival by mediating EMT may offer new therapeutic methods for preventing cancer progression.
Seasonal variations in C/N/P/K stoichiometric characteristics in different plant organs in the various forest types of Sygera Mountain
Authors: Li Yueyao , Wang Jiaxiang , Wang Luqi
Published: February 2024

We explored the resource acquisition and growth strategies of plants adapting to different environments, focusing on the typical forest types of Sygera Mountain: Pinus armandii, Picea likiangensis var. Linzhiensis, Abies georgei var. Smithii, and Juniperus saltuaria. Then, we analyzed the nutrient content and stoichiometric ratios of C, N, P, and K in different plant organs (leaves, branches, trunks, and roots) to examine the stoichiometric characteristics and nutrient balance mechanisms in these forests. Results show that within the same forest type, different plant organs exhibit high C and low N, P, and K levels. N content in all organs followed the order leaves > branches > roots > trunks. During the growth phase, the concentrations of P and K in PLL and AGS follow the order branches > leaves > roots > trunks. In the dormant phase, the distribution in different organs had the order leaves > branches > roots > trunks. C content remained relatively stable over time. In the same organ across different forest types, increase in nitrogen content in plant leaves is an active adaptation of JS plants, indicating that JS has a conservative growth strategy and can adapt to environmental stress. Owing to the influence of seasons, the evolution process of N and P content fluctuates, allocating nutrients to supporting and transporting organs for resource optimization and allocation. The N and P content were lower in the growth phase than in the dormant phase. Seasonal variations in the C/N, C/P, and C/K ratios in different forests were inversely correlated with changes in N, P, and K content in plant organs, supporting the “growth rate hypothesis.” Stoichiometric analysis suggests that different limiting elements exist in organs across various forest types. Principal component analysis indicates that the seasonal patterns of stoichiometric ratios in the organs of different forest types show species-specific characteristics, reflecting the evolutionary nutrient utilization strategies of plant genera. In summary, plant growth in different Sygera Mountain forest types is limited by N and P, with a high tendency toward nitrogen limitation. The nutrient utilization and distribution differences among various organs during different growth stages are primarily influenced by the limited availability of environmental nutrients and inherent physiological characteristics of the plants.
Flower colour and size-signals vary with altitude and resulting climate on the tropical-subtropical islands of Taiwan
Authors: Shrestha Mani , Tai King-Chun , Dyer Adrian G. , Garcia Jair E. , Yang En-Cheng , Jentsch Anke , Wang Chun-Neng
Published: February 2024

The diversity of flower colours in nature provides quantifiable evidence for how visitations by colour sensing insect pollinators can drive the evolution of angiosperm visual signalling. Recent research shows that both biotic and abiotic factors may influence flower signalling, and that harsher climate conditions may also promote salient signalling to entice scarcer pollinators to visit. In parallel, a more sophisticated appreciation of the visual task foragers face reveals that bees have a complex visual system that uses achromatic vision when moving fast, whilst colour vision requires slower, more careful inspection of targets. Spectra of 714 native flowering species across Taiwan from sea level to mountainous regions 3,300 m above sea level (a.s.l.) were measured. We modelled how the visual system of key bee pollinators process signals, including flower size. By using phylogenetically informed analyses, we observed that at lower altitudes including foothills and submontane landscapes, there is a significant relationship between colour contrast and achromatic signals. Overall, the frequency of flowers with high colour contrast increases with altitude, whilst flower size decreases. The evidence that flower colour signaling becomes increasingly salient in higher altitude conditions supports that abiotic factors influence pollinator foraging in a way that directly influences how flowering plants need to advertise.
When there is no justice, we need an old HERO. The trickle-down effect of psychological capital: the moderating role of organizational justice and leaders’ age
Authors: Gojny-Zbierowska Milena
Published: February 2024

The objective of the study was to explore the impact of leaders’ ages and followers’ perceptions of organizational justice (POJ) on the transfer of psychological capital (PsyCap) from leaders to followers, particularly examining how employees’ resilience is influenced by leaders’ PsyCap. While some evidence exists regarding PsyCap’s trickle-down effect, the specific circumstances triggering this phenomenon remain unclear. This study investigates the relationship between followers’ and leaders’ PsyCap, employing the Social Cognitive Theory and considering the moderating effects of leaders’ age and POJ. The study focused on leader-follower dyads within a randomly selected sample of 406 businesses, encompassing 812 respondents. The survey investigation utilized the CAPI approach. The hypothesized model underwent testing through multilevel dyadic regression analysis, employing an actor-partner interdependence model. The findings support two moderators —employees’ POJ and managers’ age — and highlight the cross-over effect of PsyCap. Specifically, a stronger trickle-down link is observed when a leader is older and operating within a low POJ environment. Additionally, the study revealed a positive correlation between employees’ PsyCap and POJ. The development of POJ enhances employees’ PsyCap and resilience, while leaders’ PsyCap may compensate for organizational POJ deficiencies. This study is among the first to explore PsyCap’s moderators, specifically analyzing leaders’ ages and POJ as factors influencing the cross-over effect of PsyCap. By identifying previously unrecognized moderators affecting the cross-over PsyCap effect, this research contributes significantly to the PsyCap literature.
Being there and being with them: the effects of visibility affordance of online short fitness video on users’ intention to cloud fitness
Authors: Chen Xuewei , Zhu Yuyi , Xu Xinyue
Published: February 2024

Introduction

Cloud fitness is transforming indoor exercise for young people in China. Recent studies have explored the correlation between media use and health-promoting behavior by examining the motivation of individuals and the credibility of influencers. However, the role of media affordance has thus far been largely overlooked. Drawing on the theory of Stimulus-Organism-Response (SOR), the study investigated the indirect effect of visibility affordance on the intention to exercise with fitness influencers in the context of cloud fitness through psychological variables.

Methods

This paper, based on the online survey data (N = 456), analyses the effect of visibility affordance on the intention to fitness following with influencers. A moderated parallel mediation model was constructed to examine the relationship among related variables.

Results

The paper draws the following conclusions: (1) Visibility affordance is positively related to the intention to exercise with fitness influencers. (2) Both the sense of social presence and immersion positively mediate the relationship between visibility affordance and the intention to exercise with fitness influencers. (3) The perceived popularity of the influencer positively moderates the relationship between social presence and the intention to exercise with fitness influencers and moderates the mediating role of social presence.

Discussion

Consequently, this study enhances the existing body of knowledge in exercise behavior and health communication literature, and provides practical implications for short video platform, influencers and individuals in promoting healthier behaviors.
Factors influencing coping skills of middle-aged adults in COVID-19, South Korea
Authors: Gu Minkyung , Woo Heeyoung , Sok Sohyune
Published: February 2024

Background

In light of the persistent COVID-19 pandemic, there is a compelling imperative to enhance the COVID-19 coping capacity among middle-aged adults within the South Korean population. Consequently, there is a need for further research endeavors in this area.

Objective

This study aims to explore and identify the factors influencing the coping skills of middle-aged adults in COVID-19, South Korea.

Methods

This study used a cross-sectional descriptive design. Participants were 147 middle-aged adults living in Seoul, Geounggi-do, and Chungchung-do in South Korea. Data included demographics, coping skills, the impact of the event, perceived health status, psychological wellbeing, and family support. The data were analyzed using the SPSS Statistics 25.0 program. Data were collected from March to July 2020.

Results

The regression model was significant (F = 13.56, p < 0.001), and the adjusted R-squared representing the explanatory power of the model was 0.63. The strongest predictor was perceived health status (β = 0.34), followed by family support (β = 0.31), impact of event (β = −0.24), underlying disease (β = −0.13), and economic status (β = 0.11).

Conclusion

This study suggests that to improve the coping skills of middle-aged adults with COVID-19, their perceived health status and family support should be strengthened, and the impact of the event should be decreased. Also, it needs that underlying diseases are managed and economic status is improved. Nurses need to pay more attention to the influencing factors to improve the coping skills of middle-aged adults in COVID-19, South Korea.