bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification and characterization of linear epitopes of monoclonal antibodies against the capsid proteins of small ruminant lentiviruses
Authors: Ma Xiaohua , Gao Min , Zhang Xiangmin , Ma Weiwei , Xue Fei , Wang Xue-Feng , Wang Xiaojun
Published: August 2024

Maedi-visna virus (MVV) and caprine arthritis encephalitis virus (CAEV) are members of a group of genetically highly homologous lentiviruses collectively referred to as small ruminant lentiviruses (SRLVs). SRLVs can infect sheep, goats and other small ruminants, causing multisystemic disease with progressive and persistent inflammatory changes, severely reducing animal productivity and impeding animal trade. The capsid protein of SRLVs, p28, is highly conserved among strains and is a commonly used marker for the detection of SRLVs. In this study, two monoclonal antibodies (mAbs), designated G8F7 and A10C12, against p28 were generated using a recombinant p28 protein expressed in Escherichia coli as an immunogen. Functional analysis showed that these two monoclonal antibodies could be used in iELISA, immunofluorescence assays (IFA) and western blot assays to detect p28 or Gag precursor proteins of SRLVs. Two linear epitopes, 61GNRAQKELIQGKLNEEA77 (E61-77) and 187CQKQMDRVLGTRVQQATVEEKMQACR212 (E187-212), which are recognized by G8F7 and A10C12, respectively, were identified through truncation of the GST-fused p28. Amino acid sequence alignment showed that the epitope E61-77 is conserved among SRLVs, with a dominant mutation site (K72R) that does not disrupt recognition by G8F7. E187-212 was found to exhibit variability among SRLVs, but the majority of mutant epitopes are recognized by A10C12, with the exception of a mutant epitope from an isolate with undefined subtypes from Ovis aries, which was not recognized. These findings may facilitate future study of SRLVs and promote the development of methods for the detection of these viruses.
Brain-wide functional connectivity alterations and their cognitive correlates in subjective cognitive decline
Authors: Huang Shaochun , Wang Siyu , Che Zigang , Ge Honglin , Yan Zheng , Fan Jia , Lu Xiang , Liu Li , Liu Wan , Zhong Yeming , Zou Caiyun , Rao Jiang , Chen Jiu
Published: August 2024

Background

Individuals with subjective cognitive decline (SCD) are at risk of developing Alzheimer's Disease (AD). Traditional seed-based analysis has shown biased functional connectivity (FC) in SCD individuals. To investigate unbiased altered FC by the brain-wide association study (BWAS) and to determine its association with cognition in SCD individuals.

Methods

Measure of association (MA) analysis was applied to detect significant voxels with FC changes. Based on these changes, we identified regions of interest (ROIs) and conducted ROI-wise FC analyses. Correlation analyses were then performed between these FC circuits and cognition.

Results

MA analysis identified 10 ROIs with significantly altered voxels. ROI-wise FC analyses revealed 14 strengthened FC, predominantly parietal-occipital link alterations. The FC between the right superior occipital gyrus and the right postcentral gyrus correlated positively with executive function, while the FC between the right middle occipital gyrus and the left angular gyrus correlated positively with episodic memory in SCD individuals.

Conclusion

SCD involves multifocal impairments, of which regions of default mode network (DMN) and occipital lobe should be specially focused. Cross-hemispheric alterations indicate an internal interactive impairment pattern in SCD. The reduced FC between the right superior occipital gyrus and the right postcentral gyrus, and between the right middle occipital gyrus and the left angular gyrus, which correlate with specific cognitive functions, could serve as potential biomarkers for SCD diagnosis.
Microstructural alterations in white matter and related neurobiology based on the new clinical subtypes of Parkinson's disease
Authors: Yuan Xiaorong , Yu Qiaowen , Liu Yanyan , Chen Jinge , Gao Jie , Liu Yujia , Song Ruxi , Zhang Yingzhi , Hou Zhongyu
Published: August 2024

Background and objectives

The advent of new clinical subtyping systems for Parkinson's disease (PD) has led to the classification of patients into distinct groups: mild motor predominant (PD-MMP), intermediate (PD-IM), and diffuse malignant (PD-DM). Our goal was to evaluate the efficacy of diffusion tensor imaging (DTI) in the early diagnosis, assessment of clinical progression, and prediction of prognosis of these PD subtypes. Additionally, we attempted to understand the pathological mechanisms behind white matter damage using single-photon emission computed tomography (SPECT) and cerebrospinal fluid (CSF) analyses.

Methods

We classified 135 de novo PD patients based on new clinical criteria and followed them up after 1 year, along with 45 healthy controls (HCs). We utilized tract-based spatial statistics to assess the microstructural changes of white matter at baseline and employed multiple linear regression to examine the associations between DTI metrics and clinical data at baseline and after follow-up.

Results

Compared to HCs, patients with the PD-DM subtype demonstrated reduced fractional anisotropy (FA), increased axial diffusivity (AD), and elevated radial diffusivity (RD) at baseline. The FA and RD values correlated with the severity of motor symptoms, with RD also linked to cognitive performance. Changes in FA over time were found to be in sync with changes in motor scores and global composite outcome measures. Furthermore, baseline AD values and their rate of change were related to alterations in semantic verbal fluency. We also discovered the relationship between FA values and the levels of α-synuclein and β-amyloid. Reduced dopamine transporter uptake in the left putamen correlated with RD values in superficial white matter, motor symptoms, and autonomic dysfunction at baseline as well as cognitive impairments after 1 year.

Conclusions

The PD-DM subtype is characterized by severe clinical symptoms and a faster progression when compared to the other subtypes. DTI, a well-established technique, facilitates the early identification of white matter damage, elucidates the pathophysiological mechanisms of disease progression, and predicts cognitively related outcomes. The results of SPECT and CSF analyses can be used to explain the specific pattern of white matter damage in patients with the PD-DM subtype.
The role of DEAD- and DExH-box RNA helicases in neurodevelopmental disorders
Authors: Lederbauer Johannes , Das Sarada , Piton Amelie , Lessel Davor , Kreienkamp Hans-Jürgen
Published: August 2024

Neurodevelopmental disorders (NDDs) represent a large group of disorders with an onset in the neonatal or early childhood period; NDDs include intellectual disability (ID), autism spectrum disorders (ASD), attention deficit hyperactivity disorders (ADHD), seizures, various motor disabilities and abnormal muscle tone. Among the many underlying Mendelian genetic causes for these conditions, genes coding for proteins involved in all aspects of the gene expression pathway, ranging from transcription, splicing, translation to the eventual RNA decay, feature rather prominently. Here we focus on two large families of RNA helicases (DEAD- and DExH-box helicases). Genetic variants in the coding genes for several helicases have recently been shown to be associated with NDD. We address genetic constraints for helicases, types of pathological variants which have been discovered and discuss the biological pathways in which the affected helicase proteins are involved.
Isolation, characterization, and LC MS/MS determination of anti-obesity components from pine needles of Cedrus deodara (Roxb.) G. Don
Authors: Wang Xin , Li Bin , Liu Dongyan , Guo Yuer , Zhang Jiaxu , Li Wanyu , Peng Tengteng , Ma Quhuan , Shi Xiaofeng
Published: August 2024

Objective

This study aimed to isolate and analyze the components in cedar pine needles (needle leaves of Cedrus deodara (Roxb.) G. Don) that exhibit anti-obesity effects, as determined through animal experiments.

Methods

The extract of cedar pine needles was separated into four fractions of different polarities using a macroporous resin column. The fraction that retained anti-obesity activity was evaluated based on the results of animal experiments. Monomeric compounds were structurally characterized and isolated from the active fraction using a preparative liquid chromatography system. Combined with subsequent glucose gel chromatographic separation. The content of the separated components was determined using ultrahigh performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-QQQ-MS/MS).

Results

The water-washed fraction retained anti-obesity activity of the cedar pine needles more effectively. A total of 16 compounds were separated from this fraction, and the contents of 14 of these compounds were determined to be present in cedar pine needles.

Conclusion

Nine components, namely p-hydroxy benzyl alcohol, chlorogenic acid, vanillic acid, syringic acid, P-coumaric acid, sinapic acid, benzoic acid, phenylacetic acid, salicylic acid, were characterized and determined for the first time in cedar pine needles. The components with anti-obesity activity in the pine needles of Cedrus are mainly derived from phenolic acids.
Different impacts of adipose tissue dynamics on prognosis in patients with resectable locally advanced rectal cancer treated with and without neoadjuvant treatment
Authors: Huang Weiyan , Feng Zhichao , Ma Mengtian , Song Fulong , Zeng Shumin , Shao Fang , Yu Xiaoping , Rong Pengfei , Chen Jianqiang
Published: August 2024

Background

Body composition is recognized to be associated with clinical outcomes in patients with locally advanced rectal cancer (LARC). This study aimed to determine the prognostic role of regional adipose tissue distribution in patients with resectable LARC treated with or without neoadjuvant chemoradiotherapy (nCRT).

Methods

This retrospective study included 281 consecutive patients who underwent radical surgery for LARC with or without preoperative nCRT between 2013 and 2019. Patients underwent contrast-enhanced CT scans before nCRT and before surgery. Visceral adipose tissue (VAT), abdominal subcutaneous adipose tissue (aSAT), and gluteal subcutaneous adipose tissue (gSAT) were quantified on the CT images. The association of adipose tissue distribution with progression-free survival (PFS) was analyzed using Cox proportional hazards analysis.

Results

A total of 102 nCRT-treated and 179 primarily resected patients were included. During a median follow-up period of 24 months, 74 (26.3%) patients experienced local recurrence or metastasis. Multivariable analysis showed that VAT was associated with PFS in all patients (hazard ratio [HR] 1.28, 95% confidence interval [CI] 1.04–1.57; P = 0.021). This association was only maintained in primarily resected patients (HR 1.31, 95% CI 1.02–1.69; P = 0.037). For patients receiving preoperative nCRT, VAT was not significantly associated with PFS, while the dynamic change in gSAT (ΔgSAT) between nCRT and surgery was associated with PFS (HR 0.43, 95%CI 0.27–0.69, P = 0.001).

Conclusion

Visceral obesity is an adverse prognostic factor in patients with resectable LARC treated by primary resection, while increased gluteal subcutaneous adiposity during preoperative nCRT may indicate favorable clinical outcomes.
Case report: Fast disease progression during adjuvant therapy with anti-PD-1 in stage III melanoma patients
Authors: Di Pietro Francesca Romana , Verkhovskaia Sofia , Falcone Rosa , Poti Giulia , Carbone Maria Luigia , Morelli Maria Francesca , Zappalà Albina Rita , Morese Roberto , Di Rocco Zorika Christiana , Piesco Gabriele , Chesi Paolo , Failla Cristina Maria , Marchetti Paolo , De Galitiis Federica
Published: August 2024

Background

Stage III surgically resected melanoma is a disease at high risk of recurrence. Immune checkpoint inhibitors (ICIs) and the target therapy with BRAF and MEK inhibitors significantly changed the outcome of patients with metastatic melanoma and several studies have also shown their benefit in the adjuvant setting for the delay of recurrence in stage III melanoma patients. Hyperprogression disease was observed as a possible adverse response to immunotherapy in the metastatic setting, suggesting that some patients could face additional risk of progression with ICIs, although no consensus was found for the correct definition of this event.

Case presentation

We describe here two cases of rapid multiorgan metastatization during adjuvant immunotherapy in patients with stage III resected melanoma. Even though it would be not accurate to define this syndrome as hyperprogression because of apparent absence of the initial disease in the adjuvant setting, we observed in these two cases the same very rapid progression after first administration of adjuvant ICIs that resulted in death of patients within two months from the starting of treatment. Both patients had NRAS mutated melanoma.

Conclusion

There is an urgent need for a better understanding of the causes of these fatal outcomes and for the identification of biomarkers that would allow to select the patients before offering them an adjuvant treatment, reducing the risk of hyperprogression. From these cases, we suggest that it could be useful a particular attention in proposing ICI adjuvant treatment based on the molecular profile.
Risk factors for bronchiolitis obliterans complicating adenovirus pneumonia in children: a meta-analysis
Authors: Yao Mei-mei , Gao Tian-ji , Zhao Min , Fu Yan-hua , Liu Jing , Wang Tian-jiao , Yang Ying
Published: August 2024

Objective

To preliminarily explore the risk factors for post-infectious bronchiolitis obliterans (PIBO) complicating adenovirus pneumonia (ADVP) in children through a meta-analysis.

Methods

A systematic search was conducted on three English-language databases (PubMed, Web of Science and The National Library of Medicine) and two Chinese-language databases (China National Knowledge Infrastructure and the Wanfang Database) between database inception and 1 January 2023. Data analysis was conducted using Stata 15.1 software.

Results

A total of 10 articles, reporting 14 risk factors, were included in the analysis, with 8 risk factors taken into consideration. Through the meta-analysis, 5 risk factors were identified for PIBO complicating ADVP in paediatric patients: hypoxaemia [odds ratio (OR) = 9.37, 95% CI: 4.22, 20.77, p < 0.001], persistent wheezing (OR = 4.65, 95% CI: 2.20, 9.82, p < 0.001), mechanical ventilation (OR = 3.87, 95% CI: 2.37, 6.33, p < 0.001), length of hospital stay (LoHS) (OR = 1.25, 95% CI: 1.09, 1.43, p < 0.001) and fever duration (OR = 1.08, 95% CI: 1.02, 1.14, p = 0.009).

Conclusion

Existing evidence suggests that hypoxaemia, persistent wheezing, mechanical ventilation, LoHS and fever duration are risk factors for PIBO complicating ADVP in children. These findings underscore the need for enhanced assessment and management in clinical practice. This study may provide such a clinical prediction model from the identified 5 risk factors for PIBO and offer valuable insights for preventing bronchiolitis obliterans in children with ADVP.
Rewiring of primary metabolism for ammonium recycling under short-term low CO2 treatment – its implication for C4 evolution
Authors: Miao Fenfen , Wang Ying , Haq Noor UI , Lyu Ming-Ju Amy , Zhu Xin-Guang
Published: August 2024

The dramatic decrease in atmospheric CO2 concentration during Oligocene was proposed as directly linked to C4 evolution. However, it remains unclear how the decreased CO2 concentration directly facilitate C4 evolution, besides its role as a selection pressure. We conducted a systematic transcriptomics and metabolomics analysis under short-term low CO2 condition and found that Arabidopsis grown under this condition showed 1) increased expression of most genes encoding C4-related enzymes and transporters; 2) increased expression of genes involved in photorespiration and pathways related to carbon skeleton generation for ammonium refixation; 3) increased expression of genes directly involved in ammonium refixation. Furthermore, we found that in vitro treatment of leaves with NH4+ induced a similar pattern of changes in C4 related genes and genes involved in ammonium refixation. These data support the view that Arabidopsis grown under short-term low CO2 conditions rewired its metabolism to supply carbon skeleton for ammonium recycling, during which process the expression of C4 genes were up-regulated as a result of a hitchhiking process. This study provides new insights into the adaptation of the C3 model plant Arabidopsis under low CO2 conditions and suggests that low CO2 can facilitate the evolution of C4 photosynthesis beyond the commonly assumed role of being a selection pressure.
Interaction of phosphorus and GA3 improved oilseed flax grain yield and phosphorus-utilization efficiency
Authors: Wang Yingze , Cao Zhi , Gao Yuhong , Wu Bing , Niu Junyi , Yan Bin , Wang Yifan , Cui Zhengjun , Wen Ming , Xu Peng , Wang Haidi , Ma Xingkang
Published: August 2024

Introduction

Phosphorus nutrition and hormone concentration both affect crop yield formation. Ascertaining the interaction of phosphorus and GA3 has a synergistic effect on the grain yield and phosphorus utilization efficiency of oilseed flax in dryland. It is extremely important for improving grain yield and phosphorus utilization efficiency.

Methods

A field experiment was conducted in 2019 and 2020 at the Dingxi Oil Crops Test Station to investigated the effects of phosphorus, gibberellin (GA3), and their interaction on the grain yield and phosphorus-utilization efficiency of oilseed flax plants. Phosphorus fertilizer was applied at three levels (0, 67.5, 135 kg P2O5·ha-1) and GA3 was also sprayed at three concentrations (0, 15, and 30 mg·L-1).

Results

The results showed that application of 67.5 kg P2O5·ha-1 reduced leaves acid phosphatase (ACPase) activity, but increased phosphorus accumulation throughout the growth period, the 1000-kernel weight (TKW), and the number of grains per capsule. Spraying GA3 significantly increased the leaves ACPase activity, phosphorus accumulation after anthesis and its contribution to grain, phosphorus-utilization efficiency, the number of capsules per plant, and TKW. The phosphorus accumulation at the anthesis, kernel, and maturity stages under the treatment of fertilizing 67.5 kg P2O5·ha-1 and spraying 30 mg·L-1 GA3 were increased by 56.06%, 73.51%, and 62.17%, respectively, compared with the control (no phosphorus, no GA3). And the phosphorus accumulation after anthesis and its contribution to grain also increased. 67.5 kg P2O5·ha-1 combined with 30 mg·L-1 GA3 and 135 kg P2O5·ha-1 combined with 15 mg·L-1 GA3 both significantly increased grain yield of oilseed flax, reaching 1696 kg·ha-1 and 1716 kg·ha-1 across two years, respectively. And there was no significant difference between them. However, the former treatment significant increased the apparent utilization rate, agronomic utilization rate, and partial productivity of phosphorus. The interaction between phosphorus and GA3 was significant for grain yield.

Conclusion

Therefore, the application of 67.5 kg P2O5·ha-1 in combination with 30 mg·L-1 GA3 is an effective fertilization approach for enhancing oilseed flax growth and grain yield in the experiment region and other similar areas.
Examining the issue of Indochina to understand Zhou Enlai’s neighborhood diplomacy
Authors: Qiu Yang , Qin Shan
Published: August 2024

In recent years, China’s neighboring environment has experienced profound changes, and China’s external security situation has been subject to a series of shocks. Against this background, China’s neighborhood diplomacy has become increasingly prominent. Indochina has been the focus of China’s neighborhood diplomacy since the Geneva Conference in 1954. Based on the declassified archives of Kissinger’s secret visit to China in 1971, this paper examines the issue of Indochina in the negotiations between Zhou Enlai and Kissinger. The aim is to offer a valuable historical experience for China’s neighborhood diplomacy of today and the way to manage and resolve strategic disputes in its neighboring area. The study finds that during the negotiations, Zhou took a clear stance on behalf of the Chinese government in expressing support for the struggle for the national independence of the Indochinese people. This reflects China’s unshakable commitment to the Five Principles of Peaceful Coexistence and support for the international justice of anti-aggression and anti-colonialism. Additionally, through his statements, Zhou has shown us the meaning of “amity, sincerity, mutual benefit, and inclusiveness (亲诚惠容 Qin Cheng Hui Rong)” in neighborhood diplomacy. All his efforts showed us how to effectively undertake neighborhood diplomacy, which is the essence of maintaining stability in China’s neighborhood.
Childhood adversity and depression of older adults: the moderating effect of social participation
Authors: Wang Ping , Cheng Xin , Zhang Nan , Liu Huilin
Published: August 2024

Objective

Examine the effect of childhood adversity on depression in older adults and the regulatory impact that social participation has on depression.

Methods

Based on 6,704 standard-compliant research subjects, single factor analysis, multiple linear regression model, and tendency score matching were used to analyze the impact of childhood adversity on depression in older adults and the regulatory effect of social participation.

Results

The depression rate is higher among women, young age, low education, unmarried, in agricultural households, older adults with low annual income, pre-retirement work type in agriculture, non-drinking, and those with two or more chronic diseases (p < 0.05). Children who experienced adversity as children are more likely to suffer from depression as adults (β = 0.513, 0.590, 0.954, 0.983, 1.221, 0.953, 0.718; p < 0.05). Through the tendency score, the result is matched with the endogenous test. As well, older adults are more likely to suffer psychological damage from a greater number of childhood adversities in their early years (β = 1.440, 2.646, 4.122; p < 0.001). It has been shown that social participation will reduce the negative impact of low-income family economic circumstances on depression among older adults of all ages (β = −0.459,-0.567; p < 0.01), aggravate depression resulting from “neighborhood void of mutual assistance” and “no more fun to play” for older adults of all ages (β = 1.024, 0.894; p < 0.01), and exacerbate depression resulting from “loneliness because there are no friends” for the oldest old (β = 0.476, 0.779; p < 0.05).

Conclusion

Older adults who experience childhood adversity are more likely to suffer from depression. Social participation plays a regulatory role in the relationship between childhood adversity and depression in older adults. For older adults’ mental health to improve, family and social adversity should be prevented, and moderate participation in society should be encouraged.
Predicting neuroticism with open-ended response using natural language processing
Authors: Yoon Seowon , Jang Jihee , Son Gaeun , Park Soohyun , Hwang Jueun , Choeh Joon Yeon , Choi Kee-Hong
Published: August 2024

Introduction

With rapid advancements in natural language processing (NLP), predicting personality using this technology has become a significant research interest. In personality prediction, exploring appropriate questions that elicit natural language is particularly important because questions determine the context of responses. This study aimed to predict levels of neuroticism—a core psychological trait known to predict various psychological outcomes—using responses to a series of open-ended questions developed based on the five-factor model of personality. This study examined the model’s accuracy and explored the influence of item content in predicting neuroticism.

Methods

A total of 425 Korean adults were recruited and responded to 18 open-ended questions about their personalities, along with the measurement of the Five-Factor Model traits. In total, 30,576 Korean sentences were collected. To develop the prediction models, the pre-trained language model KoBERT was used. Accuracy, F1 Score, Precision, and Recall were calculated as evaluation metrics.

Results

The results showed that items inquiring about social comparison, unintended harm, and negative feelings performed better in predicting neuroticism than other items. For predicting depressivity, items related to negative feelings, social comparison, and emotions showed superior performance. For dependency, items related to unintended harm, social dominance, and negative feelings were the most predictive.

Discussion

We identified items that performed better at neuroticism prediction than others. Prediction models developed based on open-ended questions that theoretically aligned with neuroticism exhibited superior predictive performance.
Effects of environmental phenols on eGFR: machine learning modeling methods applied to cross-sectional studies
Authors: Liu Lei , Zhou Hao , Wang Xueli , Wen Fukang , Zhang Guibin , Yu Jinao , Shen Hui , Huang Rongrong
Published: August 2024

Purpose

Limited investigation is available on the correlation between environmental phenols’ exposure and estimated glomerular filtration rate (eGFR). Our target is established a robust and explainable machine learning (ML) model that associates environmental phenols’ exposure with eGFR.

Methods

Our datasets for constructing the associations between environmental phenols’ and eGFR were collected from the National Health and Nutrition Examination Survey (NHANES, 2013–2016). Five ML models were contained and fine-tuned to eGFR regression by phenols’ exposure. Regression evaluation metrics were used to extract the limitation of the models. The most effective model was then utilized for regression, with interpretation of its features carried out using shapley additive explanations (SHAP) and the game theory python package to represent the model’s regression capacity.

Results

The study identified the top-performing random forest (RF) regressor with a mean absolute error of 0.621 and a coefficient of determination of 0.998 among 3,371 participants. Six environmental phenols with eGFR in linear regression models revealed that the concentrations of triclosan (TCS) and bisphenol S (BPS) in urine were positively correlated with eGFR, and the correlation coefficients were β = 0.010 (p = 0.026) and β = 0.007 (p = 0.004) respectively. SHAP values indicate that BPS (1.38), bisphenol F (BPF) (0.97), 2,5-dichlorophenol (0.87), TCS (0.78), BP3 (0.60), bisphenol A (BPA) (0.59) and 2,4-dichlorophenol (0.47) in urinary contributed to the model.

Conclusion

The RF model was efficient in identifying a correlation between phenols’ exposure and eGFR among United States NHANES 2013–2016 participants. The findings indicate that BPA, BPF, and BPS are inversely associated with eGFR.
Causal relationship between air pollution and infections: a two-sample Mendelian randomization study
Authors: Yang Shengyi , Tong Tong , Wang Hong , Li Zhenwei , Wang Mengmeng , Ni Kaiwen
Published: August 2024

Background

Traditional observational studies exploring the association between air pollution and infections have been limited by small sample sizes and potential confounding factors. To address these limitations, we applied Mendelian randomization (MR) to investigate the potential causal relationships between particulate matter (PM2.5, PM2.5–10, and PM10), nitrogen dioxide, and nitrogen oxide and the risks of infections.

Methods

Single nucleotide polymorphisms (SNPs) related to air pollution were selected from the genome-wide association study (GWAS) of the UK Biobank. Publicly available summary data for infections were obtained from the FinnGen Biobank and the COVID-19 Host Genetics Initiative. The inverse variance weighted (IVW) meta-analysis was used as the primary method for obtaining the Mendelian randomization (MR) estimates. Complementary analyses were performed using the weighted median method, MR-Egger method, and MR Pleiotropy Residual Sum and Outlier (MR-PRESSO) test.

Results

The fixed-effect IVW estimate showed that PM2.5, PM2.5–10 and Nitrogen oxides were suggestively associated with COVID-19 [for PM2.5: IVW (fe): OR 3.573(1.218,5.288), PIVW(fe) = 0.021; for PM2.5–10: IVW (fe): OR 2.940(1.385,6.239), PIVW(fe) = 0.005; for Nitrogen oxides, IVW (fe): OR 1.898(1.318,2.472), PIVW(fe) = 0.010]. PM2.5, PM2.5–10, PM10, and Nitrogen oxides were suggestively associated with bacterial pneumonia [for PM2.5: IVW(fe): OR 1.720 (1.007, 2.937), PIVW(fe) = 0.047; for PM2.5–10: IVW(fe): OR 1.752 (1.111, 2.767), P IVW(fe) = 0.016; for PM10: IVW(fe): OR 2.097 (1.045, 4.208), PIVW(fe) = 0.037; for Nitrogen oxides, IVW(fe): OR 3.907 (1.209, 5.987), PIVW(fe) = 0.023]. Furthermore, Nitrogen dioxide was suggestively associated with the risk of acute upper respiratory infections, while all air pollution were not associated with intestinal infections.

Conclusions

Our results support a role of related air pollution in the Corona Virus Disease 2019, bacterial pneumonia and acute upper respiratory infections. More work is need for policy formulation to reduce the air pollution and the emission of toxic and of harmful gas.
Community resilience through partnership after the Great East Japan Earthquake: cooking classes by Iwate Co-op and a food company
Authors: Ito Naomi , Konno Kayoko , Nozaki Kumiko , Fukushi Kumiko , Kanno Kasumi , Kawamura Hiromi , Nakamura Yayoi , Yamada Mikio , Kuroda Ai , Kuchii Toshiya , Kinoshita Yuri , Nabetani Teru , Fukuda Yoshiharu
Published: August 2024

Introduction

This case study aimed to demonstrate how cooking class activities held in collaboration with the Ajinomoto Foundation (TAF) and a consumer cooperative after the Great East Japan Earthquake contributed to the resilience of the affected community.

Methods

With reference to the logic model, evaluation indicators for the case study were established. We focused on the Iwate Seikatsu Kyodo Kumiai (Iwate Co-op). We organized 120 continuation cases out of the eight-and-a-half-year activity records of the project owned by TAF (April 2012 to March 2020). The Iwate Co-op was one of the 120 continuing cases and had special features, including awareness that its members were responsible for the community.

Results

The collaboration revealed three effects. First, owing to the encounter and collaboration with TAF, the cooking class was continuously conducted even after TAF withdrew from the disaster-affected areas. Second, the Iwate Co-op trained some of its members as food support staff to run the cooking class independently and was actively involved in obtaining the necessary budget for the operation, consequently leading to the independent activation of member activities. Third, they developed a cooking class project in inland areas other than disaster-stricken areas, assuming that they could incorporate the project into their existing activities, as food problems affected people beyond disaster victims.

Conclusion

The collaborative food support project of the Iwate Co-op and TAF contributed to the resilience of the affected people and communities by strengthening bonds and solidarity among residents and organizations. The key to success was the fusion of a traditional sense of independence in the co-op with TAF’s mission and technical know-how. The partnership between the Iwate Co-op and TAF allowed the former to aim toward developing food support activities in the affected areas, accelerating the resilience of the community in the Iwate Prefecture.
Real-time temperature anomaly detection in vaccine refrigeration systems using deep learning on a resource-constrained microcontroller
Authors: Harrabi Mokhtar , Hamdi Abdelaziz , Ouni Bouraoui , Bel Hadj Tahar Jamel
Published: August 2024

Maintaining consistent and accurate temperature is critical for the safe and effective storage of vaccines. Traditional monitoring methods often lack real-time capabilities and may not be sensitive enough to detect subtle anomalies. This paper presents a novel deep learning-based system for real-time temperature fault detection in refrigeration systems used for vaccine storage. Our system utilizes a semi-supervised Convolutional Autoencoder (CAE) model deployed on a resource-constrained ESP32 microcontroller. The CAE is trained on real-world temperature sensor data to capture temporal patterns and reconstruct normal temperature profiles. Deviations from the reconstructed profiles are flagged as potential anomalies, enabling real-time fault detection. Evaluation using real-time data demonstrates an impressive 92% accuracy in identifying temperature faults. The system’s low energy consumption (0.05 watts) and memory usage (1.2 MB) make it suitable for deployment in resource-constrained environments. This work paves the way for improved monitoring and fault detection in refrigeration systems, ultimately contributing to the reliable storage of life-saving vaccines.
mini-Complexome Profiling (mCP), an FDR-controlled workflow for global targeted detection of protein complexes
Authors: Amedei Hugo , Paul Niels Benjamin , Foo Brian , Neuenroth Lisa , Lehnart Stephan E. , Urlaub Henning , Lenz Christof
Published: August 2024

Introduction

Co-fractionation mass spectrometry couples native-like separations of protein-protein complexes with mass spectrometric proteome analysis for global characterization of protein networks. The technique allows for both de novo detection of complexes and for the detection of subtle changes in their protein composition. The typical requirement for fine-grained fractionation of >80 fractions, however, translates into significant demands on sample quantity and mass spectrometric instrument time, and represents a significant barrier to experimental replication and the use of scarce sample material (ex. patient biopsies).

Methods

We developed mini-Complexome Profiling (mCP), a streamlined workflow with reduced requirements for fractionation and, thus, biological material and laboratory and instrument time. Soluble and membrane-associated protein complexes are extracted from biological material under mild conditions, and fractionated by Blue Native electrophoresis using commercial equipment. Each fraction is analysed by data-independent acquisition mass spectrometry, and known protein complexes are detected based on the coelution of known components using a novel R package with a controlled false discovery rate approach. The tool is available to the community on a GitHub repository.

Results

mCP was benchmarked using HEK293 cell lysate and exhibited performance similar to established workflows, but from a significantly reduced number of fractions. We then challenged mCP by performing comparative complexome analysis of cardiomyocytes isolated from different chambers from a single mouse heart, where we identified subtle chamber-specific changes in mitochondrial OxPhos complexes.

Discussion

The reduced sample and instrument time requirements open up new applications of co-fractionation mass spectrometry, specifically for the analysis of sparse samples such as human patient biopsies. The ability to identify subtle changes between similar tissue types (left/right ventricular and atrial cardiomyocytes) serves as a proof of principle for comparative analysis of mild/asymptomatic disease states.
Sensory processing associated with subcategories of restricted and repetitive behaviors in Japanese children and adolescents with autism spectrum disorder
Authors: Noda Haruka , Yoneda Naoto , Kamogawa Ken , Tanaka Goro , Ide Masakazu , Iwanaga Ryoichiro
Published: August 2024

Background

Restricted and repetitive behavior (RRB) is a core symptom of autism spectrum disorder (ASD). The structure of RRB subcategories and their relationship with atypical sensory processing in Japan are not well understood. This study examined subcategories of the RRB in Japanese children with ASD and explored their relationship with sensory processing.

Methods

A total of 103 children and adolescents with ASD participated in this study, with more than 70% having a co-occurring intellectual disability. First, exploratory factor analysis of the RRB items of the Social Responsiveness Scale second edition (SRS-2) was conducted to identify RRB subcategories. Second, Spearman correlation and multiple regression analysis were run to examine relationships between the RRB subcategories of SRS-2 and subsections of the Short Sensory Profile.

Results

Exploratory factor analysis indicated a two factors solution; repetitive sensory and motor behavior and insistence on sameness. Multiple regression analysis suggested that Movement Sensitivity and Auditory Filtering were associated with insistence on sameness. Furthermore, Underresponsive/Seeks Sensation, Visual/Auditory Sensitivity, and diagnosis of intellectual disabilities were associated with repetitive sensory and motor behavior.

Conclusions

Findings indicate that RRB subcategories are differently related to sensory processing patterns in children with ASD. These results suggested that RRB subcategories are beneficial to consider the relationship between RRB and sensory processing.
Drug assumption and awareness about adverse drug reactions. The right to know. The case of the bone-modyfing agents: a systematic review
Authors: Mauceri Rodolfo , Arduini Sonia , Coppini Martina , Bazzano Monica , Trujillo Isabel , Campisi Giuseppina
Published: August 2024

Introduction

Medication-related osteonecrosis of the Jaw (MRONJ) is an adverse drug reaction that affects the mandible and maxilla of patients exposed to BMA and AA therapies, causing the progressive destruction and death of bone. To date, oral health preventive measures remain the most effective strategy to reduce MRONJ incidence, and, in this sense, the major goal is to diagnose, treat, and eradicate any oral diseases that could compromise oral health. The present systematic review aims to investigate the awareness of MRONJ among patients assuming BMAs.

Methods

A systematic literature search was performed, selecting studies that concern the awareness of patients of the risk of MRONJ.

Results

Six studies were included in this review. In total, 483 patients were evaluated. Of the 483 included patients, 391 were not aware of the possibility of MRONJ onset (391/483, 81%) and 92 were aware of it (92/483, 19%).

Discussion

The problem of patient's lack of awareness with respect to MRONJ risk presents different layers of complexity (“what?”, “who?”, “where?”, “when?” and “why?”). Among its causal factors, there are an inadequate level of communication with patients and the lack of collaboration between healthcare professionals, which is related to an individualistic view of liability and deontological duties. MRONJ is a drug adverse reaction that can greatly affect the quality of life of patients if not promptly diagnosed and treated. Therefore, patients must be fully aware of the risks of adverse and the importance of preventive measures, which imply effective and exhaustive communication by each member of the multidisciplinary team. Effective teamwork and collaborative care should be promoted to positively impact patients' awareness.
A qualitative approach to explore the cognitive processes used by members of an adult learn-to-cycle program
Authors: Franks Caitlyn , Reis Arianne C. , Peel Nicole , Steel Kylie Ann
Published: August 2024

Introduction

Attaining movement proficiency under various constraints is well-researched; of particular interest here is how conscious processing and self-consciousness influence learning and performance. Current research relevant to these variables e.g., reinvestment, tends to utilize quantitative methods and thus overlooks a potentially rich source of understanding. Therefore, the purpose of this study was to apply a qualitative approach to explore the cognitive processes and self-consciousness within a less practiced population of adults participating in a non-competitive leisure activity.

Methods

To achieve this, eleven semi-structured interviews were conducted with adult women learning or relearning to ride a bicycle.

Results

Using thematic analysis, two distinct themes were evident and corroborated those illustrated in scales such as the movement-specific reinvestment scale. These themes included Conscious Processing, Recalling Experiences and Movement Analysis, in addition to Style of Movement, and Self-Consciousness. Anxiety also emerged as an additional and relevant theme to learning a new complex movement skill.

Discussion

The findings indicated that participating women attuned to their movements to the extent that there was a conscious attempt to control their movements, but less so regarding movements being altered by self-awareness. Whilst further research is required, qualitative methods provide a promising basis for exploring the cognitive process involved with the conscious process involved in learning.
The effect of electro-magnetic-energy-regulation therapy on subjective sleep among elite players in Norwegian women's football
Authors: Moen Frode , Pettersen Svein Arne , Mosleth Ellen F.
Published: August 2024

The current study investigated if Bio-Electro-Magnetic-Energy-Regulation (BEMER) therapy is affecting subjective sleep among a sample of 21 elite female football players in a Norwegian top series club. Subjective sleep was measured each day over a period of 273 consecutive days by using a smartphone application, PM Reporter Pro, which scheduled push messages to remind the participants to report sleep every morning. The study was conducted as a quasi-experimental design, with a control period before the introduction of BEMER therapy that lasted for 3 months, followed by an intervention period where BEMER therapy was used that lasted for 5 months. The collected data from the players in the control period served as their control compared to the data collected from the players in the intervention period. Analyses of variance (ANOVA) with False Discovery Rate adjusted p-values show that subjective sleep duration and subjective sleep quality are significantly reduced on game nights, both in the control period and in the experiment period. The results also show that subjective sleep duration and subjective sleep quality significantly increase in the experiment period compared to the control period. The findings indicate that BEMER therapy might serve as a tool to improve sleep.
Gender diversity in pediatric surgery: academic ranks and scholarly productivity amongst pediatric surgeons
Authors: Mullis Danielle M. , Mueller Claudia , Bonham Spencer A. , Hunt Emily , Uribe Daniela , Miller Hayley , Bianco Y. Katherine
Published: August 2024

Purpose

Despite a growing number of women entering medical school, a small proportion of women pursue surgical specialties, including pediatric surgery. This multi-center study assesses gender-based differences in measures of scholarly productivity and distribution of faculty positions.

Methods

This is a retrospective web-based study of all pediatric surgeons at twelve large institutions across the United States. Data published by the American Association of Medical Colleges was compiled and analyzed to understand the gender distributions of medical students, general surgery residents, and pediatric surgery fellows. P-values were calculated using two-sided Student's independent t-tests and chi-squared tests.

Results

There have been a growing number of women applying into pediatric surgery, but the proportion of women matriculating into these fellowships is not concordant. Women are still underrepresented (28%) amongst the pediatric surgeon workforce. A total of 111 pediatric surgeons were identified for this study, which included 31 women (28%) and 80 males (72%). There was a significant difference in the distribution across academic ranks between genders (p < 0.001). Women had significantly fewer publications per year after residency, fewer total publications, and a lower h-index in comparison to men (p < .001, p = .005, p = .002, respectively).

Conclusions

Women are not only underrepresented in pediatric surgery, but there are also significant differences in the distribution of faculty positions and scholarly productivity when comparing men and women. There is a pressing need to improve gender diversity and identify barriers that may prevent women from advancing to leadership positions and achieving professional success.
Comparative effects of nisin and monensin supplementation on growth performance, rumen fermentation, nutrient digestion, and plasma metabolites of fattening Hu sheep
Authors: Luo Jiazhi , Jiang Jun , Duan Hongwei , Zhang Han , Sun Meijie , Mao Shengyong , Shen Junshi
Published: August 2024

Introduction

This study was conducted to compare the effects of nisin (NIS) and ionophore antibiotic monensin (MON) on the growth performance, rumen fermentation, nutrient digestion and plasma metabolites of fattening Hu sheep.

Methods

Thirty-six male Hu sheep (23.5 ± 1.0 kg) were divided into two blocks based on BW (low BW and high BW). Sheep within each block were then allotted to 9 pens respectively (two sheep/pen). Pens within each block were randomly assigned to one of three dietary treatments: (1) basal diet (CON); (2) basal diet + 40 mg/kg DM of MON; (3) basal diet + 274.5 mg/kg DM of NIS. The study lasted 9 weeks, with the initial 2 weeks for adaptation and the subsequent 7 weeks for treatment.

Results

The results showed that both NIS and MON addition had no impacts on average daily gain (ADG), dry matter intake (DMI), and feed conservation rate (G:F) of sheep (p > 0.05). The digestibility of ether extract (EE) was lower in the MON-fed and NIS-fed sheep (p < 0.01) than in the CON group, whereas crude protein (CP) digestibility was higher in the MON-fed sheep compared to those fed NIS (p < 0.05). Both NIS and MON supplementation decreased acetate levels and acetate/propionate ratio in the rumen of Hu sheep (p < 0.05). Sheep fed MON exhibited higher total cholesterol concentrations (p < 0.05) compared to the CON and NIS groups. However, there were no significant differences in other plasma metabolites, including blood urea nitrogen (BUN), total bile acid, triglyceride, total protein, albumin, globulin, glucose, etc., among the three groups (p > 0.05).

Discussion

In conclusion, dietary addition of NIS and MON altered the rumen fermentation mode by reducing acetate levels, with no discernible effects on the growth performance of the fattening Hu sheep.
Donor-Derived Cell-Free DNA as a Companion Biomarker for AMR Treatment With Daratumumab: Case Series
Authors: Osmanodja Bilgin , Akifova Aylin , Budde Klemens , Oellerich Michael , Beck Julia , Bornemann-Kolatzki Kirsten , Schütz Ekkehard , Velden Joachim , Lehmann Claudia , Krüger Bastian Malte , Bachmann Anette , Kowald Jan
Published: August 2024

Antibody-mediated rejection (AMR) is among the most frequent causes for graft loss after kidney transplantation. While there are no approved therapies, several case reports with daratumumab and the very recent phase 2 trial of felzartamab in AMR have indicated the potential efficacy of therapeutic interventions targeting CD38. Donor-derived cell-free DNA (dd-cfDNA) is an emerging biomarker with injury-specific release and a short half-life, which could facilitate early diagnosis of AMR and monitoring of treatment response. We describe two cases of patients with chronic active AMR, who were treated with monthly daratumumab infusions, and in whom donor-derived cell-free DNA (dd-cfDNA) was measured longitudinally to monitor treatment response. In both patients, daratumumab treatment led to stabilization of kidney function parameters, a strong decline of dd-cfDNA below the previously established threshold for rejection, and partial or complete histologic resolution of AMR activity. Our case series suggests that dd-cfDNA may be a useful companion biomarker for longitudinal monitoring of anti-CD38 treatment in patients with AMR.