bluuebunny/crossref_metadata_2025 huggingface dataset:
Alveolar macrophage modulation via the gut–lung axis in lung diseases
Authors: Chen Zijian , Liu Yangqi , Huang Weizhe
Published: November 2023

Several studies have demonstrated great potential implications for the gut–lung axis in lung disease etiology and treatment. The gut environment can be influenced by diet, metabolites, microbiotal composition, primary diseases, and medical interventions. These changes modulate the functions of alveolar macrophages (AMs) to shape the pulmonary immune response, which greatly impacts lung health. The immune modulation of AMs is implicated in the pathogenesis of various lung diseases. However, the mechanism of the gut–lung axis in lung diseases has not yet been determined. This mini-review aimed to shed light on the critical nature of communication between the gut and AMs during the development of pulmonary infection, injury, allergy, and malignancy. A better understanding of their crosstalk may provide new insights into future therapeutic strategies targeting the gut–AM interaction.
Molecular and functional changes in neutrophilic granulocytes induced by nicotine: a systematic review and critical evaluation
Authors: Brembach Theresa-Charlotte , Sabat Robert , Witte Katrin , Schwerdtle Tanja , Wolk Kerstin
Published: November 2023

Background

Over 1.1 billion people smoke worldwide. The alkaloid nicotine is a prominent and addictive component of tobacco. In addition to tumors and cardiovascular disorders, tobacco consumption is associated with a variety of chronic-inflammatory diseases. Although neutrophilic granulocytes (neutrophils) play a role in the pathogenesis of many of these diseases, the impact of nicotine on neutrophils has not been systematically reviewed so far.

Objectives

The aim of this systematic review was to evaluate the direct influence of nicotine on human neutrophil functions, specifically on cell death/damage, apoptosis, chemotaxis, general motility, adhesion molecule expression, eicosanoid synthesis, cytokine/chemokine expression, formation of neutrophil extracellular traps (NETs), phagocytosis, generation of reactive oxygen species (ROS), net antimicrobial activity, and enzyme release.

Material and methods

This review was conducted according to the PRISMA guidelines. A literature search was performed in the databases NCBI Pubmed® and Web of Science™ in February 2023. Inclusion criteria comprised English written research articles, showing in vitro studies on the direct impact of nicotine on specified human neutrophil functions.

Results

Of the 532 originally identified articles, data from 34 articles were finally compiled after several evaluation steps. The considered studies highly varied in methodological aspects. While at high concentrations (>3 mmol/l) nicotine started to be cytotoxic to neutrophils, concentrations typically achieved in blood of smokers (in the nmol/l range) applied for long exposure times (24-72h) supported the survival of neutrophils. Smoking-relevant nicotine concentrations also increased the chemotaxis of neutrophils towards several chemoattractants, elevated their production of elastase, lipocalin-2, CXCL8, leukotriene B4 and prostaglandin E2, and reduced their integrin expression. Moreover, while nicotine impaired the neutrophil phagocytotic and anti-microbial activity, a range of studies demonstrated increased NET formation. However, conflicting effects were found on ROS generation, selectin expression and release of β-glucuronidase and myeloperoxidase.

Conclusion

Nicotine seems to support the presence in the tissue and the inflammatory and selected tissue-damaging activity of neutrophils and reduces their antimicrobial functions, suggesting a direct contribution of nicotine to the pathogenesis of chronic-inflammatory diseases via influencing the neutrophil biology.
Elucidating the dynamic immune responses within the ocular mucosa of rainbow trout (Oncorhynchus mykiss) after infection with Flavobacterium columnare
Authors: Kong Weiguang , Yang Peng , Ding Guangyi , Cheng Gaofeng , Xu Zhen
Published: November 2023

The eye of vertebrates is constantly faced with numerous challenges from aquatic or airborne pathogens. As a crucial first line of defense, the ocular mucosa (OM) protects the visual organ from external threats in vertebrates such as birds and mammals. However, the understanding of ocular mucosal immunity in early vertebrates, such as teleost fish, remains limited, particularly concerning their resistance to bacterial infections. To gain insights into the pivotal role of the OM in antibacterial immunity among teleost fish, we developed a bacterial infection model using Flavobacterium columnare in rainbow trout (Oncorhynchus mykiss). Here the qPCR and immunofluorescence results showed that F. columnare could invade trout OM, suggesting that the OM could be a primary target and barrier for the bacteria. Moreover, immune-related genes (il-6, il-8, il-11, cxcl10, nod1, il1-b, igm, igt, etc.) were upregulated in the OM of trout following F. columnare infection, as confirmed by qPCR, which was further proved through RNA-seq. The results of transcriptome analyses showed that bacterial infection critically triggers a robust immune response, including innate, and adaptive immune-related signaling pathways such as Toll-like, NOD-like, and C-type lectin receptor signaling pathway and immune network for IgA production, which underscores the immune role of the OM in bacterial infection. Interestingly, a substantial reduction in the expression of genes associated with visual function was observed after infection, indicating that bacterial infection could impact ocular function. Overall, our findings have unveiled a robust mucosal immune response to bacterial infection in the teleost OM for the first time, providing valuable insights for future research into the mechanisms and functions of ocular mucosal immunity in early vertebrate species.
Role of the co-stimulatory molecule inducible T-cell co-stimulator ligand (ICOSL) in the progression of experimental metabolic dysfunction-associated steatohepatitis
Authors: Provera Alessia , Ramavath Naresh Naik , Gadipudi Laila Lavanya , Gigliotti Casimiro Luca , Boggio Elena , Vecchio Cristina , Stoppa Ian , Rolla Roberta , Boldorini Renzo , Pirisi Mario , Smirne Carlo , Albano Emanuele , Dianzani Umberto , Sutti Salvatore
Published: November 2023

Background and aims

Inducible T-cell Co-Stimulator (ICOS) present on T-lymphocytes and its ligand ICOSL expressed by myeloid cells play multiple roles in regulating T-cell functions. However, recent evidence indicates that reverse signalling involving ICOSL is also important in directing the differentiation of monocyte-derived cells. In this study, we investigated the involvement of ICOS/ICOSL dyad in modulating macrophage functions during the evolution of metabolic dysfunction-associated steatohepatitis (MASH).

Results

In animal models of MASH, ICOS was selectively up-regulated on CD8+ T-cells in parallel with an expansion of ICOSL-expressing macrophages. An increase in circulating soluble ICOSL was also evident in patients with MASH as compared to healthy individuals. ICOSL knockout (ICOSL-/-) mice receiving choline/methionine deficient (MCD) diet for 6 weeks had milder steatohepatitis than wild type mice. MASH improvement was confirmed in mice fed with cholesterol-enriched Western diet for 24 weeks in which ICOSL deficiency greatly reduced liver fibrosis along with the formation of crown-like macrophage aggregates producing the pro-fibrogenic mediators osteopontin (OPN) and galectin-3 (Gal-3). These effects associated with a selective shewing of F4-80+/CD11bhigh monocyte-derived macrophages (MoMFs) expressing the Triggering Receptor Expressed on Myeloid cells 2 (TREM2) to CD11blow/F4-80+ cells positive for the Kupffer cell marker C-type lectin-like type 2 receptor (CLEC-2), thus indicating an increased MoMF maturation toward monocyte-derived Kupffer cells.

Conclusions

These results suggest that CD8+ T-cells interaction with monocyte-derived macrophages through ICOS/ICOSL critically supports a specific subset of TREM2+-expressing cells contributing to the evolution of steatohepatitis. The data also point ICOS/ICOSL dyad as a possible target for therapeutic interventions in MASH.
Research progress on the immune microenvironment and immunotherapy in gastric cancer
Authors: Mou Pei , Ge Qing-hua , Sheng Rong , Zhu Teng-fei , Liu Ye , Ding Kai
Published: November 2023

The tumor microenvironment, particularly the immune microenvironment, plays an indispensable role in the malignant progression and metastasis of gastric cancer (GC). As our understanding of the GC microenvironment continues to evolve, we are gaining deeper insights into the biological mechanisms at the single-cell level. This, in turn, has offered fresh perspectives on GC therapy. Encouragingly, there are various monotherapy and combination therapies in use, such as immune checkpoint inhibitors, adoptive cell transfer therapy, chimeric antigen receptor T cell therapy, antibody-drug conjugates, and cancer vaccines. In this paper, we review the current research progress regarding the GC microenvironment and summarize promising immunotherapy research and targeted therapies.
Integration of liquid biopsy and immunotherapy: opening a new era in colorectal cancer treatment
Authors: Yao Shiya , Han Yuejun , Yang Mengxiang , Jin Ketao , Lan Huanrong
Published: November 2023

Immunotherapy has revolutionized the conventional treatment approaches for colorectal cancer (CRC), offering new therapeutic prospects for patients. Liquid biopsy has shown significant potential in early screening, diagnosis, and postoperative monitoring by analyzing circulating tumor cells (CTC) and circulating tumor DNA (ctDNA). In the era of immunotherapy, liquid biopsy provides additional possibilities for guiding immune-based treatments. Emerging technologies such as mass spectrometry-based detection of neoantigens and flow cytometry-based T cell sorting offer new tools for liquid biopsy, aiming to optimize immune therapy strategies. The integration of liquid biopsy with immunotherapy holds promise for improving treatment outcomes in colorectal cancer patients, enabling breakthroughs in early diagnosis and treatment, and providing patients with more personalized, precise, and effective treatment strategies.
Immunotherapies inducing immunogenic cell death in cancer: insight of the innate immune system
Authors: Calvillo-Rodríguez Kenny Misael , Lorenzo-Anota Helen Yarimet , Rodríguez-Padilla Cristina , Martínez-Torres Ana Carolina , Scott-Algara Daniel
Published: November 2023

Cancer immunotherapies include monoclonal antibodies, cytokines, oncolytic viruses, cellular therapies, and other biological and synthetic immunomodulators. These are traditionally studied for their effect on the immune system’s role in eliminating cancer cells. However, some of these therapies have the unique ability to directly induce cytotoxicity in cancer cells by inducing immunogenic cell death (ICD). Unlike general immune stimulation, ICD triggers specific therapy-induced cell death pathways, based on the release of damage-associated molecular patterns (DAMPs) from dying tumour cells. These activate innate pattern recognition receptors (PRRs) and subsequent adaptive immune responses, offering the promise of sustained anticancer drug efficacy and durable antitumour immune memory. Exploring how onco-immunotherapies can trigger ICD, enhances our understanding of their mechanisms and potential for combination strategies. This review explores the complexities of these immunotherapeutic approaches that induce ICD, highlighting their implications for the innate immune system, addressing challenges in cancer treatment, and emphasising the pivotal role of ICD in contemporary cancer research.
Expansion of CD4+ cytotoxic T lymphocytes with specific gene expression patterns may contribute to suppression of tumor immunity in oral squamous cell carcinoma: single-cell analysis and in vitro experiments
Authors: Chen Hu , Sameshima Junsei , Yokomizo Shiho , Sueyoshi Tomoki , Nagano Haruki , Miyahara Yuka , Sakamoto Taiki , Fujii Shinsuke , Kiyoshima Tamotsu , Guy Thomas , Nakamura Seiji , Moriyama Masafumi , Kaneko Naoki , Kawano Shintaro
Published: November 2023

Background

Cancer immunotherapy targeting CD8+ T cells has made remarkable progress, even for oral squamous cell carcinoma (OSCC), a heterogeneous epithelial tumor without a substantial increase in the overall survival rate over the past decade. However, the therapeutic effects remain limited due to therapy resistance. Thus, a more comprehensive understanding of the roles of CD4+ T cells and B cells is crucial for more robust development of cancer immunotherapy.

Methods

In this study, we examined immune responses and effector functions of CD4+ T cells, CD8+ T cells and B cells infiltrating in OSCC lesions using single-cell RNA sequencing analysis, T cell receptor (TCR) and B cell receptor (BCR) repertoire sequencing analysis, and multi-color immunofluorescence staining. Finally, two Kaplan-Meier curves and several Cox proportional hazards models were constructed for the survival analysis.

Results

We observed expansion of CD4+ cytotoxic T lymphocytes (CTLs) expressing granzymes, which are reported to induce cell apoptosis, with a unique gene expression patterns. CD4+ CTLs also expressed CXCL13, which is a B cell chemoattractant. Cell–cell communication analysis and multi-color immunofluorescence staining demonstrated potential interactions between CD4+ CTLs and B cells, particularly IgD- CD27- double negative (DN) B cells. Expansion of CD4+ CTLs, DN B cells, and their contacts has been reported in T and B cell-activated diseases, including IgG4-related disease and COVID-19. Notably, we observed upregulation of several inhibitory receptor genes including CTLA-4 in CD4+ CTLs, which possibly dampened T and B cell activity. We next demonstrated comprehensive delineation of the potential for CD8+ T cell differentiation towards dysfunctional states. Furthermore, prognostic analysis revealed unfavorable outcomes of patients with a high proportion of CD4+ CTLs in OSCC lesions.

Conclusion

Our study provides a dynamic landscape of lymphocytes and demonstrates a systemic investigation of CD4+ CTL effects infiltrating into OSCC lesions, which may share some pathogenesis reported in severe T and B cell-activated diseases such as autoimmune and infectious diseases.
A non-coding variant in SLC15A4 modulates enhancer activity and lysosomal deacidification linked to lupus susceptibility
Authors: Singh Manish Kumar , Maiti Guru Prashad , Rallabandi Harikrishna Reddy , Fazel-Najafabadi Mehdi , Looger Loren L. , Nath Swapan K.
Published: November 2023

Background

Systemic lupus erythematosus (SLE) is a complex autoimmune disease with a strong genetic basis. Despite the identification of several single nucleotide polymorphisms (SNPs) near the SLC15A4 gene that are significantly associated with SLE across multiple populations, specific causal SNP(s) and molecular mechanisms responsible for disease susceptibility are unknown.

Methods

To address this gap, we employed bioinformatics, expression quantitative trait loci (eQTLs), and 3D chromatin interaction analysis to nominate a likely functional variant, rs35907548, in an active intronic enhancer of SLC15A4.

Results

Through luciferase reporter assays followed by chromatin immunoprecipitation (ChIP)-qPCR, we observed significant allele-specific enhancer effects of rs35907548 in diverse cell lines. The rs35907548 risk allele T is associated with increased regulatory activity and target gene expression, as shown by eQTLs and chromosome conformation capture (3C)-qPCR. The latter revealed long-range chromatin interactions between the rs35907548 enhancer and the promoters of SLC15A4, GLTLD1, and an uncharacterized lncRNA. The enhancer-promoter interactions and expression effects were validated by CRISPR/Cas9 knock-out (KO) of the locus in HL60 promyeloblast cells. KO cells also displayed dramatically dysregulated endolysosomal pH regulation.

Conclusions

Together, our data show that the rs35907548 risk allele affects multiple aspects of cellular physiology and may directly contribute to SLE.
Transboundary maritime cooperation: the case of the Eastern Caribbean Region
Authors: Mahadeo Sarah , Del Savio Linda
Published: November 2023

Transboundary cooperation is a priority for the Organisation of Eastern Caribbean States (OECS). For over thirty years since its formation, it has pursued policies of integration and cooperation among its members with the aim of promoting development and reducing social and economic inequalities across the region. Within the last decade, the islands have embarked on ambitious plans for a sustainable Blue Economy, centred on developing the potential of their vast maritime zones. Achieving this requires cooperation across maritime borders for the management of shared natural resources and transnational human activities, as well as for addressing transboundary challenges facing the region such as climate change, marine pollution and Sargassum influxes. Through a literature review and documentary analysis this article provides a first stocktaking of transboundary maritime cooperation in the region through a mapping of regional level policy promoting cooperation and projects among OECS members over the last decade. The analysis reveals great diversity among participating countries, thematic priorities and actors operating across multiple governance levels. The article concludes with a discussion on the drivers for cooperation among OECS members, highlighting transboundary maritime cooperation as a natural and logical approach for capitalising on opportunities and addressing challenges in a capacity constrained region.
Microzooplankton communities and their grazing of phytoplankton under artificial upwelling in the oligotrophic ocean
Authors: Spilling Kristian , Arellano San Martín Mirian , Granlund Mira , Schulz Kai G. , Spisla Carsten , Vanharanta Mari , Goldenberg Silvan Urs , Riebesell Ulf
Published: November 2023

Ocean artificial upwelling has been suggested to boost primary production and increase harvestable resources such as fish. Yet, for this ecosystem-based approach to work, an effective energy transfer up the food web is required. Here, we studied the trophic role of microzooplankton under artificial upwelling via biomass and community composition as well as grazing rates on phytoplankton. Using mesocosms in the oligotrophic ocean, we supplied nutrient-rich deep water at varying intensities (low to high) and addition modes (a Singular large pulse or smaller Recurring pulses). Deep-water fertilization created a diatom-dominated bloom that scaled with the amount of inorganic nutrients added, but also Synechococcus-like cells, picoeukaryotes and nanophytoplankton increased in abundance with added nutrients. After 30 days, towards the end of the experiment, coccolithophores bloomed under recurring upwelling of high intensity. Across all upwelling scenarios, the microzooplankton community was dominated by ciliates, dinoflagellates (mixo- and heterotrophic) and radiolarians. Under the highest upwelling intensity, the average grazing rates of Synechococcus-like cells, picoeukaryotes and nanophytoplankton by microzooplankton were 0.35 d-1 ± 0.18 (SD), 0.09 d-1 ± 0.12 (SD), and 0.11 d-1 ± 0.13 (SD), respectively. There was little temporal variation in grazing of nanophytoplankton but grazing of Synechococcus-like cells and picoeukaryotes were more variable. There were positive correlations between abundance of these groups and grazing rates, suggesting a response in the microzooplankton community to prey availability. The average phytoplankton to microzooplankton ratio (biovolume) increased with added deep water, and this increase was highest in the Singular treatment, reaching ~30 (m3 m-3), whereas the phytoplankton to total zooplankton biomass ratio (weight) increased from ~1 under low upwelling to ~6 (g g-1) in the highest upwelling but without a difference between the Singular and the Recurring mode. Several smaller, recurring upwelling events increased the importance of microzooplankton compared with one large pulse of deep water. Our results demonstrate that microzooplankton would be an important component for trophic transfer if artificial upwelling would be carried out at scale in the oligotrophic ocean.
In situ carbon uptake of marine macrophytes is highly variable among species, taxa, and morphology
Authors: Jacobs Julian M. , Himes Lucian , La Valle Florybeth F.
Published: November 2023

Macroalgae form important coastal ecosystems and are considered to be highly productive, yet individual macrophyte carbon uptake rates are poorly documented and methodologies for in situ assessments of productivity are not well developed. In this study, we employ a 13C enrichment method in benthic chambers to calculate carbon uptake rates and assess δ13C signatures of a large stock of nearshore benthic macroalgae varying in taxa and morphology in Southern California. Our objectives are to 1) identify the variability of carbon uptake and inorganic carbon use among individuals of the same species or morphology, and 2) establish accurate and accessible carbon uptake procedures for coastal benthic primary producers. We found no significant relationship between the observed ranges of environmental factors such as nutrient concentrations, PAR, temperature, conductivity, and productivity rates, suggesting that unique physiological complexions underpin the high variability of carbon uptake and δ13C in studied macrophyte samples. We consider three reasons our experimental carbon uptake rates are 3–4 orders of magnitude lower than existing literature, which reports carbon uptake in the same units despite using different methods: 1) underrepresentation of Pmax, 2) incomplete carbon fractionation corrections, and 3) reduced hydrodynamics within the benthic chambers.
Enhancing the ocean carbon sink capacity of Shandong province, China, under the “dual carbon” goal
Authors: Mao Zhengkai , Liu Huirong , Zhang Zhijun
Published: November 2023

The ocean is a key component of the global carbon cycle. As climate change becomes increasingly severe, enhancing the ocean’s carbon sink capacity is of great significance to achieving the “dual carbon” goal for China. As a major maritime province, Shandong is in an advantageous position to utilize the ocean to respond to climate change and achieve the national goals of carbon peaking and carbon neutrality. Focusing on consolidating and enhancing ocean carbon sink capabilities, Shandong needs to formulate a clear timetable and roadmap to promote blue carbon technology research and development, actively cultivate a blue carbon trading market, and tap the inner potential of ocean carbon sink. Shandong should also strengthen international cooperation to jointly construct a carbon-neutral global community.
Chitosan- hypothalamic hormonal analogue nanoconjugates enhanced the reproductive performance in Indian major carp, Labeo rohita
Authors: Malik Mohd Ashraf , Gupta Subodh , Varghese Tincy , Jahageerdar Shrinivas , Nayak Sunil Kumar , Reang Dhalongsaih , Bhat Irfan Ahmad , Mahadevaswamy Chandan Gowramma , Chuphal Nisha , Dar Showkat Ahmad , Prabhakaran Arya
Published: November 2023

The current study reports the potential of chitosan nanoparticles for the efficient delivery of hypothalamic hormonal analogue in Labeo rohita, aiming to improve reproductive performance. Here salmon gonadotropin-releasing hormone analogue (sGnRH-a) was conjugated with chitosan nanoparticles (ChN-sGnRH-a) to evaluate its efficiency in enhancing the reproductive gene expression, hormones as well as the overall reproductive output in L. rohita. A total of 54 pairs of brooders were selected and divided into six treatments viz; C0: Negative Control (fish injected with bare chitosan nanoparticles), C: Positive Control (fish injected with Gonopro-FH®, a commercially available inducing hormone, at a dose of 0.2 ml/kg body weight of fish), T1: fish injected with ChN-sGnRH-a at a dose of 0.2 ml/kg, T2: T1 + 10 mg/ml domperidone, T3: fish injected with ChN-sGnRH-a at a dose of 0.1 ml/kg, T4: T3 + 10 mg/ml domperidone. In T2 and T4 treatments, serum hormones, Testosterone (T); Estradiol (E2); Vitellogenin (Vtg); and 17α, 20β-dihydroxyprogesterone (17α, 20β-DHP) showed the sustained elevation. The means of reproductive traits like fecundity, fertilization rate, pseudo-gonadosomatic index and spawning rate were significantly (P<0.05) higher in T2 treatment, while no significant difference was found between C and T4 treatment. The mRNA expression level of follicle-stimulating hormone (fshβ), luteinizing hormone (lhβ) and their cognate receptors was significantly better in T2 treatment, while no significant difference was found between T4 and C treatments. Further, the histological analysis of ovaries showed increased post-ovulatory follicles in C, T2 and T4 treatments. The results indicate that ChN-sGnRH-a could help in reducing the recommended dose of sGnRH-a without affecting the reproductive performance in L. rohita females. The sustained releasing mechanism of this formulation may be used as an induced breeding strategy in female L. rohita broodstock.
Layer combination design and effect evaluation of phase change cooling asphalt pavement
Authors: Huang Peng , Fan Dong , Li Dawei , Gong Xiangbing , Jiang Yu , Liu Ziming , Huang Xue
Published: November 2023

Asphalt mixture is a temperature sensitive material. With the change of external environment temperature, asphalt pavement is prone to temperature-related diseases. Adding phase change material (PCM) to asphalt pavement to adjust pavement temperature is one of the effective methods at present. At present, there are many types of research on PCMs, but the research on PCMs added to the pavement structure is very scarce. In this paper, through the temperature test of rutting plate specimens with different layer combinations, the cooling effect of pavement structure combinations with PCMs added to different layers (upper layer, middle layer, and bottom layer) in pavement structure under different illumination times are discussed. Through the self-designed environmental simulation box, the real-time monitoring of the temperature of different layers in the pavement structure is realized. The cooling effect between different layers in different phase change pavement structure combinations is analyzed, and compared with each layer of ordinary pavement structure, and the best addition method is obtained for phase change materials, which provides a certain reference for the construction and specific application of PCM asphalt pavement, and made important contributions to the development of asphalt cooling pavement. The results show that the PCM can effectively reduce the temperature of each layer of the pavement structure. Under different illumination durations, the cooling effect of the samples with PCM in the upper layer was the worst. The samples with phase change material in the middle layer had the best cooling effect in the upper and middle layers of the pavement. The addition of phase change material to both the upper and middle layers had the most obvious cooling effect on the lower layer of the pavement. Therefore, combined with the comprehensive consideration of economy and cooling effect, the comprehensive cooling effect of adding PCMs to the middle surface layer is the best. It is recommended to add phase change materials to the middle surface layer during asphalt cooling pavement construction.
Design and performance evaluation of a novel broadband THz modulator based on graphene metamaterial for emerging applications
Authors: Tiang Jun Jiat , Soliman Naglaa F. , Khan Imran , Choi Jaeyoung , Chung Hee Chan , Madsen Dag Øivind
Published: November 2023

Introduction: Metamaterials consist of periodic arrangements of artificial subwavelength units that possess electromagnetic properties not present in natural media. It has attracted more interest due to its ability to alter electromagnetic radiation in a flexible manner, which has resulted in the development of multiple radio frequency devices based on metamaterials. Metamaterials with the required frequency band for electric or magnetic resonance can be made using unit cell structure. The incident electromagnetic wave will enter the metamaterials and be kept there in the absence of reflection.

Methods: This paper proposes a novel broadband THz absorber filter based on graphene for emerging applications. The proposed structure comprised of three parts. The top layer consists of graphene, the middle layer consists of dielectric and the bottom layer is made up of gold.

Results: The proposed structure is experimentally designed and validated using the COMSOL simulator.

Discussion: Simulation results show that the proposed absorber has better performance as compared with existing methods.
Numerical simulation study on the effects of liquid water atomization on the flow field and performance of aluminum-based water ramjet engines
Authors: Zhang Yuntian , Wu Yunkai , Cao Xiwei , Liu Yuanshu , Sun Yongqiang , Yang Jing , Junli Liu
Published: November 2023

In order to investigate the effects of different water inlet droplet diameters on the performance of aluminum-based water ramjet engines, the internal flow field of the engine was analyzed through numerical simulation. The results showed that by selecting a suitable water droplet diameter at the water inlet and controlling the time required for water droplet evaporation and heat absorption, the working range of aluminum-water combustion reaction can be expanded and the specific impulse of the engine can be increased. In engine design and practical application, the design of the water injection nozzle upstream of the engine is critical, and the droplet diameter at the water inlet should be controlled within a suitable range. A diameter that is too large will reduce the evaporation efficiency and hinder the further diffusion of combustion reaction. Droplet sizes that are too small will rapidly evaporate, causing the temperature in the flow field to decrease rapidly, leading to a large range of low-temperature regions in the main reaction zone of the combustion chamber, thereby reducing the overall aluminum-water reaction rate of the engine. In addition, the variation of droplet diameter in the downstream water atomization nozzle has little effect on the aluminum-water reaction in the main combustion zone. However, reducing the droplet diameter can facilitate the downstream diffusion of the combustion reaction, further expanding the combustion range and increasing the specific impulse. Furthermore, it can also reduce the temperature near the wall, which is beneficial for reducing the overall thermal protection requirements of the engine.
Dual-mode 3D printed dynamic wrist driven orthosis for hand therapy exercises
Authors: Risangtuni Ayu Gareta , Suprijanto S. , Nazaruddin Yul Yunazwin , Mahyuddin Andi I.
Published: November 2023

The primary objective of the Dual-mode Dynamic Wrist Driven Orthosis (D-WDO) is to facilitate wrist-hand therapy exercises for patients with varying levels of residual muscle function. This dual-mode D-WDO system comprises two main components: the orthosis structure and the soft pneumatic actuator (SPA). All system components were designed and produced using Computer Aided Design (CAD) software and the Fused Deposition Modeling (FDM) 3D printing technique. The D-WDO’s structure is constructed from PLA (Polylactic Acid), while the SPA is made from TPU (Thermoplastic Polyurethane) filament. The D-WDO can be operated in passive or active mode by attaching or detaching the SPA from the structure. This D-WDO system is particularly suitable for patients with a minimum MMT level between 2 and 3, as it provides assistance for wrist movement and supports repetitive wrist motion to enhance wrist muscle function. However, it is important to note that the operation and performance of the dual-mode D-WDO system may vary depending on the chosen system configuration. The active D-WDO’s performance demonstrates its ability to achieve the necessary wrist flexion angle for a functional wrist joint, especially in the context of daily activities.
ABO-incompatible living donor kidney transplantation failure due to acute blood group antibody-dependent rejection triggered by human parvovirus B19 infection: a case report and literature review
Authors: Dai Lin-rui , Wang Xiao-hui , Hou Yi-bo , Zou Zhi-yu , Chen Song , Zhang Wei-jie , Chang Sheng
Published: November 2023

Background

With the improvement of immunosuppressive regimens, the success rate and availability of ABO-incompatible (ABO-i) kidney transplantation (KT) have gradually increased. However, the management of immunosuppression protocols and complications associated with ABO-i KT is complex. Here, we report a clinical case of ABO-i living donor KT with allograft dysfunction caused by acute blood group antibody-dependent rejection triggered by human parvovirus B19 (B19V).

Case report

The ABO blood group of the recipient was O, and that of the donor was B. The recipient had high baseline anti-B antibody titers (IgM, 1:1024; IgG, 1:64). Before transplantation, he completed a desensitization protocol comprising plasma exchange, double-filtration plasmapheresis, and rituximab, which maintained a low blood group antibody level and resulted in successful transplantation. Two weeks after surgery, the recipient developed a B19V infection combined with acute T-cell-mediated rejection. After the anti-rejection regimen, acute rejection (AR) was successfully reversed, but B19V persisted. One week after AR stabilization, the patient experienced acute antibody-mediated rejection that was more severe and refractory, resulting in the loss of the transplanted kidney.

Conclusion

Desensitization combined with immunosuppressants can lead to overimmunosuppression and cause various infections. Infections could break the accommodation state of the patient, thereby inducing AR and resulting in the loss of the transplanted kidney.
Case report: Fatal Legionella infection diagnosed via by metagenomic next-generation sequencing in a patient with chronic myeloid leukemia
Authors: Bu Chunhong , Lei Shuai , Chen Linguang , Xie Yanqiu , Zheng Guoli , Hua Liwei
Published: November 2023

Legionella is an aerobic, gram-negative, intracellular pathogen and is an important cause of community-acquired pneumonia. Legionella pneumophila is the most common causative agent of Legionella pneumonia. Clinical diagnosis of Legionella pneumonia is challenging due to the lack of specific clinical manifestations and the low positive rates of conventional pathogen detection methods. In this study, we report a case of a patient with chronic myeloid leukemia who developed rigors and high fever after chemotherapy and immunotherapy. Chest computed tomography revealed consolidation in the left lower lobe of the lung and ground-glass opacities in both lower lobes. Multiple blood cultures showed Escherichia coli, Staphylococcus aureus, Bacillus licheniformis, and positive results in the β-D-glucan test (G test). The patient was treated with various sensitive antimicrobial agents, including meropenem plus fluconazole, meropenem plus carpofungin, and vancomycin. Unfortunately, the patient’s condition gradually worsened and eventually resulted in death. On the following day of death, metagenomic next-generation sequencing (mNGS) of 1whole blood revealed L. pneumophila pneumonia with concurrent bloodstream infection (blood mNGS reads 114,302). These findings suggest that when conventional empirical antimicrobial therapy proves ineffective for critically ill patients with pneumonia, the possibility of combined Legionella infection must be considered, and mNGS can provide a diagnostic tool in such cases.
Therapeutic efficacy of optimal pulse technology in the treatment of chalazions
Authors: Song Xi , Zhang Chunying , Zhang Saisai , He Mansha
Published: November 2023

Introduction

To evaluate the efficacy of optimized pulse technology in treating chalazia.

Methods

Prospective before-after study. All patients received two sessions of optimal pulse technology (OPT) with an interval of 1 week. The first visit was before treatment and the patients underwent 2 treatment sessions with a 1-week interval. The non-invasive tear breakup time (NIBUT), corneal fluorescein staining (CFS) score, Schirmer’s test I without anesthesia, conjunctival hyperemia, and meibomian gland area were compared before and after treatment, and the related factors of curative effect were analyzed.

Results

23 patients (23 eyes) with chalazia were included. All patients received two sessions of OPT treatment at 1-week intervals. Following the first OPT treatment, a reduction in the chalazion size was observed in 17 patients (73.91%). One patient was completely cured, and 1 patient had an increase in the diameter of the chalazion. The meibomian gland area increased significantly compared to before treatment (p = 0.023). Compared with baseline, the conjunctival congestion and ST decreased, NIBUT increased, and there was no statistical difference. After the second treatment, the chalazion size decreased in 21 cases, and 3 patients were cured. A significant increase in the meibomian gland area compared with the baseline area (p < 0.001). Additionally, conjunctival congestion decreased significantly. After two sessions, the Schirmer test exhibited a decrease, and NIBUT increased, although these changes did not reach statistical significance. The curative effect was unrelated to sex, age, first onset, single disease, and other factors.

Conclusion

After treatment, the diameter of chalazions was reduced in 91.3% of the patients, and the area of the meibomian gland was significantly increased compared with that before treatment, which suggested that 2 OPT treatments at an interval of 1 week can improve the signs of adult patients in the non-acute infectious stage with chalazia.
Peripheral blood stem cell transplantation vs. bone marrow transplantation for aplastic anemia: a systematic review and meta-analysis
Authors: Zhang Zhao , Zhou Xianghui , Cheng Zhipeng , Hu Yu
Published: November 2023

Background

Hematopoietic stem cell transplantation (HSCT) is an effective treatment for aplastic anemia. Recently, peripheral blood stem cell transplantation (PBSCT) has gradually replaced traditional bone marrow transplantation (BMT). However, which graft source has a better therapeutic effect and prognosis for aplastic anemia (AA) remains unclear. Therefore, we conducted this systematic review and meta-analysis.

Methods

We systematically searched PubMed, EMBASE, and the Cochrane Library without language limitations for studies using PBSCT or BMT for AA. Data were analyzed using the Open Meta-Analyst.

Results

We identified 17 of 18,749 studies, including seven comparative reports and nine single-arm reports, with a total of 3,516 patients receiving HSCT (1,328 and 2,188 patients received PBSCT and BMT, respectively). The outcomes of the comparative studies showed similar 5-year overall survival [OS; relative risk (RR) = 0.867; 95% confidence interval (CI), 0.747–1.006], similar transplant-related mortality (RR = 1.300; 95%CI, 0.790–2.138), graft failure rate (RR = 0.972; 95%CI, 0.689–1.372) between the PBSCT group and the BMT group, while the PBSCT group had a significantly higher incidence of chronic graft-versus-host disease (GVHD; RR = 1.796; 95% CI, 1.571–2.053) and a higher incidence of grade IV acute GVHD (RR = 1.560; 95% CI, 1.341–1.816) compared to the BMT group. The outcomes of single-arm reports showed similar 3-year OS and incidences of chronic GVHD, acute II–IV GVHD, III–IV GVHD, transplant-related mortality and graft failure rate between PBSCT and BMT.

Conclusion

Before 2010, PBSCT was not superior to BMT in terms of 5-year OS, transplant-related mortality and graft failure rate, but it exhibited a higher risk of both chronic and acute GVHD. After 2010, PBSCT and BMT showed similar 3-year OS, GVHD risks, transplant-related mortality and graft failure rate. PB grafts are more suitable for HSCT of the AA for convenience and pain relief.

Systematic review registration

www.crd.york.ac.uk/PROSPERO/, CRD42023412467.
Exploring large language model for next generation of artificial intelligence in ophthalmology
Authors: Jin Kai , Yuan Lu , Wu Hongkang , Grzybowski Andrzej , Ye Juan
Published: November 2023

In recent years, ophthalmology has advanced significantly, thanks to rapid progress in artificial intelligence (AI) technologies. Large language models (LLMs) like ChatGPT have emerged as powerful tools for natural language processing. This paper finally includes 108 studies, and explores LLMs’ potential in the next generation of AI in ophthalmology. The results encompass a diverse range of studies in the field of ophthalmology, highlighting the versatile applications of LLMs. Subfields encompass general ophthalmology, retinal diseases, anterior segment diseases, glaucoma, and ophthalmic plastics. Results show LLMs’ competence in generating informative and contextually relevant responses, potentially reducing diagnostic errors and improving patient outcomes. Overall, this study highlights LLMs’ promising role in shaping AI’s future in ophthalmology. By leveraging AI, ophthalmologists can access a wealth of information, enhance diagnostic accuracy, and provide better patient care. Despite challenges, continued AI advancements and ongoing research will pave the way for the next generation of AI-assisted ophthalmic practices.
The realistic positioning of UVA1 phototherapy after 25 years of clinical experience and the availability of new biologics and small molecules: a retrospective clinical study
Authors: Calzavara-Pinton Piergiacomo , Bettolini Luca , Tonon Francesco , Rossi Mariateresa , Venturini Marina
Published: November 2023

Background

Since the early 1990s, Ultraviolet (UV) A1 phototherapy has been described as an effective and safe treatment of a multitude of skin disorders. However, after 30 years, its use has remained limited to few dermatological centers.

Objective

To analyze the changes over the years and the current position of UVA1 phototherapy through a Real-World Evidence (RWE) study at a single tertiary referral center.

Methods

We reviewed the medical files of 740 patients treated between 1998 and 2022. Treatment results were collected, efficacy was assessed by a grading scale and acute adverse effects were registered.

Results

We treated patients with 26 different diseases. We registered marked improvement (MI) or complete remission (CR) in 42.8% of patients with morphea, 50% with Urticaria Pigmentosa, 40.7% with Granuloma annulare and 85.7% with skin sarcoidosis. Good results were obtained also in the treatment of chronic Graft Versus Host Disease (GVHD), Eosinophilic Fasciitis, Sclero-atrophic Lichen, skin manifestations of systemic lupus erythematosus and psoriasis of HIV+ patients. Systemic Sclerosis, Romberg’s Syndrome, Bushke’s Scleredema, Nephrogenic Fibrosing Dermopathy, REM Syndrome, Follicular Mucinosis, Pretibial Myxedema, Scleromyxedema, pemphigus foliaceus, chronic cutaneous lupus erythematosus, erythroderma of Netherton Syndrome and Necrobiosis Lipoidica were no or poorly responsive. In clinical indications where UVA1 was used as a second line phototherapy after narrow-band (NB)-UVB, we saw good MI or CR rates in Mycosis Fungoides (57% of patients), Atopic Dermatitis (33.9%), Pitiryasis Lichenoides chronica (50%), Pityriasis Lichenoides et varioliformis acute (75%) and Lymphomatod Papulosis (62.5%). Short-term adverse events were uncommon and mild.

Conclusion

Over the past decade, the annual number of treated patients has progressively declined for several reasons. Firstly, UVA1 phototherapy has taken a backseat to the cheaper and more practical NB-UVB phototherapy, which has proven effective for common indications. Secondly, the emergence of new, safe, and effective drugs for conditions such as atopic dermatitis, GVHD, and connective tissue disorders. Finally, our research has shown that UVA1 therapy is often ineffective or minimally effective for some rare diseases, contrary to previous case reports and small case series. Nonetheless, UVA1 continues to be a valuable treatment option for patients with specific skin disorders.
Microbacterium spp. peritonitis in patients undergoing peritoneal dialysis: a single-center experience and literature review
Authors: Yu Xiao , Li Zhao , Yang Xinbo , Wang Xiaopei , Nan Di , Lu Wanhong , Lv Jing , Ma Ying
Published: November 2023

Introduction

Peritoneal dialysis-related peritonitis (PDRP) caused by Microbacterium spp. is very rare, with only 9 cases reported to date. In this study, we report the treatment experiences of 7 patients at our peritoneal dialysis center.

Methods

We retrospectively collected clinical characteristics and antibiotic management of all 7 episodes of PDRP caused by Microbacterium spp. in 7 patients from at our center over 4 years, and reviewed the documented Microbacterium spp. PDRP in the literature.

Results

Empiric antibiotic therapy was initiated as soon as possible, and consisted of intraperitoneal (IP) gentamicin in combination with vancomycin. After up to 5 days, gentamicin was changed to meropenem if the treatment was not effective. The intended course of antibiotic treatment was 21-day. Totally, 6 episodes were cured (85.7%), which was higher than reported.

Conclusion

The 21-day antibiotic therapy program by combining vancomycin and meropenem may benefit the management of Microbacterium spp. PDRP.
Droplet-based methodology for investigating bacterial population dynamics in response to phage exposure
Authors: Nikolic Nela , Anagnostidis Vasileios , Tiwari Anuj , Chait Remy , Gielen Fabrice
Published: November 2023

An alarming rise in antimicrobial resistance worldwide has spurred efforts into the search for alternatives to antibiotic treatments. The use of bacteriophages, bacterial viruses harmless to humans, represents a promising approach with potential to treat bacterial infections (phage therapy). Recent advances in microscopy-based single-cell techniques have allowed researchers to develop new quantitative methodologies for assessing the interactions between bacteria and phages, especially the ability of phages to eradicate bacterial pathogen populations and to modulate growth of both commensal and pathogen populations. Here we combine droplet microfluidics with fluorescence time-lapse microscopy to characterize the growth and lysis dynamics of the bacterium Escherichia coli confined in droplets when challenged with phage. We investigated phages that promote lysis of infected E. coli cells, specifically, a phage species with DNA genome, T7 (Escherichia virus T7) and two phage species with RNA genomes, MS2 (Emesvirus zinderi) and Qβ (Qubevirus durum). Our microfluidic trapping device generated and immobilized picoliter-sized droplets, enabling stable imaging of bacterial growth and lysis in a temperature-controlled setup. Temporal information on bacterial population size was recorded for up to 25 h, allowing us to determine growth rates of bacterial populations and helping us uncover the extent and speed of phage infection. In the long-term, the development of novel microfluidic single-cell and population-level approaches will expedite research towards fundamental understanding of the genetic and molecular basis of rapid phage-induced lysis and eco-evolutionary aspects of bacteria-phage dynamics, and ultimately help identify key factors influencing the success of phage therapy.