bluuebunny/crossref_metadata_2025 huggingface dataset:
TRITEX: chromosome-scale sequence assembly of Triticeae genomes with open-source tools
Authors: Monat Cécile , Padmarasu Sudharsan , Lux Thomas , Wicker Thomas , Gundlach Heidrun , Himmelbach Axel , Ens Jennifer , Li Chengdao , Muehlbauer Gary J. , Schulman Alan H. , Waugh Robbie , Braumann Ilka , Pozniak Curtis , Scholz Uwe , Mayer Klaus F. X. , Spannagl Manuel , Stein Nils , Mascher Martin
Published: December 2019

Chromosome-scale genome sequence assemblies underpin pan-genomic studies. Recent genome assembly efforts in the large-genome Triticeae crops wheat and barley have relied on the commercial closed-source assembly algorithm DeNovoMagic. We present TRITEX, an open-source computational workflow that combines paired-end, mate-pair, 10X Genomics linked-read with chromosome conformation capture sequencing data to construct sequence scaffolds with megabase-scale contiguity ordered into chromosomal pseudomolecules. We evaluate the performance of TRITEX on publicly available sequence data of tetraploid wild emmer and hexaploid bread wheat, and construct an improved annotated reference genome sequence assembly of the barley cultivar Morex as a community resource.
Continuous intravenous low-dose diclofenac sodium to control a central fever after ischemic stroke in the intensive care unit: a case report and review of the literature
Authors: Giaccari L. G. , Pace M. C. , Passavanti M. B. , Sansone P. , Esposito V. , Aurilio C. , Pota V.
Published: December 2019

Introduction

Elevation in body temperature within the first 24 hours of ischemic stroke is fairly common and known to be associated with worse outcomes. Only after thoroughly ruling out infection and the noninfectious etiologies and in the appropriate clinical setting should the diagnosis of central fever be made. Acetaminophen and nonsteroidal anti-inflammatory drugs are typical therapeutic options. External cooling is frequently used when pharmacologic interventions are inadequate. However, reports have suggested that neurogenic fevers are somewhat resistant to traditional pharmacologic therapies.

Case presentation

We describe a case of a Caucasian patient with central fever after ischemic stroke not responsive to acetaminophen administration and external cooling. After an initial bolus of diclofenac sodium (0.2 mg/kg in 100 ml of saline solution for 30 minutes), a continuous infusion (75 mg in 50 ml of saline solution) was started. After 5 days of treatment, the patient’s body temperature was below 37.5 °C, and the diclofenac sodium infusion was stopped.

Conclusions

We observed that a low-dose diclofenac sodium infusion was effective in treating fever without systemic side effects. This treatment may be suggested as an alternative to conventional antipyretic drugs, but additional clinical trials are required.
LncRNA Neat1 mediates miR-124-induced activation of Wnt/β-catenin signaling in spinal cord neural progenitor cells
Authors: Cui Yi , Yin Yanyun , Xiao Zhifeng , Zhao Yannan , Chen Bing , Yang Bin , Xu Bai , Song Hongwei , Zou Yunlong , Ma Xu , Dai Jianwu
Published: December 2019

Background

Emerging evidence suggests that miR-124 performs important biological functions in neural stem cells (NSCs); it regulates NSC behavior and promotes the differentiation of NSCs into neurons, but the exact molecular mechanism remains unknown. And also, the role of miR-124 during spinal cord injury regeneration is unclear.

Materials and methods

In order to explore the function of miR-124 in neural differentiation, the molecular markers (Tuj1, Map2, and GFAP) correlated with the differentiation of NSCs were detected by immunofluorescence staining both in cultured mouse spinal cord progenitor cells (SC-NPCs) and in spinal cord injury (SCI) animal models. The migration ability and apoptosis of cultured SC-NPCs were also evaluated by Transwell migration assay and TUNEL assay. In addition, the relative expression of lnRNA Neat1- and Wnt/β-catenin signaling-related genes were detected by quantitative real-time PCR.

Results

In this study, we revealed that lncRNA Neat1 is involved in regulating Wnt/β-catenin signaling that is activated by miR-124 in SC-NPCs. LncRNA Neat1 was also found to play an important role in regulating neuronal differentiation, apoptosis, and migration of SC-NPCs. Furthermore, we demonstrated that overexpression of miR-124 resulted in elevated Neat1 expression, accompanied with the functional recovery of locomotion in a mouse model of spinal cord injury.

Conclusions

Our results confirm the therapeutic effectiveness of miR-124 on the functional recovery of injured spinal cord, supporting the rationale and feasibility of miR-124 for spinal cord injury treatment in future clinical therapy. Furthermore, we concluded that the miR-124-Neat1-Wnt/β-catenin signaling axis is involved in regulating the cell function of SC-NPCs, and this may offer novel therapeutic avenues for future treatment of SCI.
Combination therapies enhance immunoregulatory properties of MIAMI cells
Authors: Rossi Fiorella , Noren Hunter , Sarria Leonor , Schiller Paul C. , Nathanson Lubov , Beljanski Vladimir
Published: December 2019

Background

Mesenchymal stromal cells (MSCs), adult stromal cells most commonly isolated from bone marrow (BM), are being increasingly utilized in various therapeutic applications including tissue repair via immunomodulation, which is recognized as one of their most relevant mechanism of action. The promise of MSC-based therapies is somewhat hindered by their apparent modest clinical benefits, highlighting the need for approaches that would increase the efficacy of such therapies. Manipulation of cellular stress-response mechanism(s) such as autophagy, a catabolic stress-response mechanism, with small molecules prior to or during MSC injection could improve MSCs’ therapeutic efficacy. Unfortunately, limited information exists on how manipulation of autophagy affects MSCs’ response to inflammation and subsequent immunoregulatory properties.

Methods

In this study, we exposed BM-MSC precursor cells, “marrow-isolated adult multilineage inducible” (MIAMI) cells, to autophagy modulators tamoxifen (TX) or chloroquine (CQ), together with IFN-γ. Exposed cells then underwent RNA sequencing (RNAseq) to determine the effects of TX or CQ co-treatments on cellular response to IFN-γ at a molecular level. Furthermore, we evaluated their immunoregulatory capacity using activated CD4+ T cells by analyzing T cell activation marker CD25 and the percentage of proliferating T cells after co-culturing the cells with MIAMI cells treated or not with TX or CQ.

Results

RNAseq data indicate that the co-treatments alter both mRNA and protein levels of key genes responsible for MSCs’ immune-regulatory properties. Interestingly, TX and CQ also altered some of the microRNAs targeting such key genes. In addition, while IFN-γ treatment alone increased the surface expression of PD-L1 and secretion of IDO, this increase was further enhanced with TX. An improvement in MIAMI cells’ ability to decrease the activation and proliferation of T cells was also observed with TX, and to a lesser extent, CQ co-treatments.

Conclusion

Altogether, this work suggests that both TX and CQ have a potential to enhance MIAMI cells’ immunoregulatory properties. However, this enhancement is more pronounced with TX co-treatment.
Bone marrow-derived neural crest precursors improve nerve defect repair partially through secreted trophic factors
Authors: Shi Haiyan , Li Xiaoli , Yang Junling , Zhao Yahong , Xue Chengbin , Wang Yaxian , He Qianru , Shen Mi , Zhang Qi , Yang Yumin , Ding Fei
Published: December 2019

Background

Emerging evidence suggests that neural crest-derived cells (NCCs) present important functions in peripheral nerve regeneration to correct the insufficiency of autogenous Schwann cells. Postmigratory NCCs have been successfully isolated from adult rat bone marrow in our previous work. In this study, we aim to provide neural crest-derived Schwann cell precursors (SCPs) for repair of nerve defects in adult rats, and partially reveal the mechanisms involved in neuroregeneration of cell therapy.

Methods

A clonal cell line of neural crest precursors of rat bone marrow origin (rBM-NCPs) with SCP identity was expanded in adherent monolayer culture to ensure the stable cell viability of NCPs and potentiate the repair of nerve defects after rBM-NCPs implantation based on tissue engineering nerve grafts (TENG). Here the behavioral, morphological, and electrophysiological detection was performed to evaluate the therapy efficacy. We further investigated the treatment with NCP-conditioned medium (NCP-CM) to sensory neurons after exposure to oxygen-glucose-deprivation (OGD) and partially compared the expression of trophic factor genes in rBM-NCPs with that in mesenchymal stem cells of bone marrow origin (rBM-MSCs).

Results

It was showed that the constructed TENG with rBM-NCPs loaded into silk fibroin fiber scaffolds/chitosan conduits repaired 10-mm long sciatic nerve defects more efficiently than conduits alone. The axonal regrowth, remyelination promoted the reinnervation of the denervated hind limb muscle and skin and thereby alleviated muscle atrophy and facilitated the rehabilitation of motor and sensory function. Moreover, it was demonstrated that treatment with NCP-CM could restore the cultured primary sensory neurons after OGD through trophic factors including epidermal growth factor (EGF), platelet-derived growth factor alpha (PDGFα), ciliary neurotrophic factor (CNTF), and vascular endothelial growth factor alpha (VEGFα).

Conclusions

In summary, our findings indicated that monolayer-cultured rBM-NCPs cell-based therapy might effectively repair peripheral nerve defects partially through secreted trophic factors, which represented the secretome of rBM-NCPs differing from that of rBM-MSCs.
Gastrointestinal colonization with multidrug-resistant Gram-negative bacteria during extracorporeal membrane oxygenation: effect on the risk of subsequent infections and impact on patient outcome
Authors: Grasselli Giacomo , Scaravilli Vittorio , Alagna Laura , Bombino Michela , De Falco Stefano , Bandera Alessandra , Abbruzzese Chiara , Patroniti Nicolò , Gori Andrea , Pesenti Antonio
Published: December 2019

Background

In ICU patients, digestive tract colonization by multidrug-resistant (MDR) Gram-negative (G−) bacteria is a significant risk factor for the development of infections. In patients undergoing extracorporeal membrane oxygenation (ECMO), colonization by MDR bacteria and risk of subsequent nosocomial infections (NIs) have not been studied yet. The aim of this study is to evaluate the incidence, etiology, risk factors, impact on outcome of gastrointestinal colonization by MDR G− bacteria, and risk of subsequent infections in patients undergoing ECMO.

Methods

This is a retrospective analysis of prospectively collected data: 105 consecutive patients, treated with ECMO, were admitted to the ICU of an Italian tertiary referral center (San Gerardo Hospital, Monza, Italy) from January 2010 to November 2015. Rectal swabs for MDR G− bacteria were cultured at admission and twice a week. Only colonization and NIs by MDR G− bacteria were analyzed.

Results

Ninety-one included patients [48.5 (37–56) years old, 63% male, simplified acute physiology score II 37 (32–47)] underwent peripheral ECMO (87% veno-venous) for medical indications (79% ARDS). Nineteen (21%) patients were colonized by MDR G− bacteria. Male gender (OR 4.03, p = 0.029) and duration of mechanical ventilation (MV) before ECMO > 3 days (OR 3.57, p = 0.014) were associated with increased risk of colonization. Colonized patients had increased odds of infections by the colonizing germs (84% vs. 29%, p < 0.001, OR 12.9), longer ICU length of stay (LOS) (43 vs. 24 days, p = 0.002), MV (50 vs. 22 days, p < 0.001) and ECMO (28 vs. 12 days, p < 0.001), but did not have higher risk of death (survival rate 58% vs. 67%, p = 0.480, OR 0.68). Infected patients had almost halved ICU survival (46% vs. 78%, p < 0.001, OR 4.11).

Conclusions

In patients undergoing ECMO for respiratory and/or circulatory failure, colonization by MDR G− bacteria is frequent and associated with more the tenfold odds for subsequent infection. Those infections are associated with an increased risk of death.
On stability of generalized (affine) phase retrieval in the complex case
Authors: Zhuang Zhitao
Published: December 2019

In this paper, we discuss the stability of generalized phase retrieval and generalized affine phase retrieval in the complex case. By the realification method, we obtain the bi-Lipschitz property in the absence of noise case and Cramer–Rao lower bound under noise conditions.
Existence and uniqueness of solutions for system of Hilfer–Hadamard sequential fractional differential equations with two point boundary conditions
Authors: Saengthong Warissara , Thailert Ekkarath , Ntouyas Sotiris K.
Published: December 2019

In this paper, we study existence and uniqueness of solutions for a system of Hilfer–Hadamard sequential fractional differential equations via standard fixed point theorems. The existence is proved by using the Leray–Schauder alternative, while the existence and uniqueness by the Banach contraction mapping principle. Illustrative examples are also discussed.
Waist height ratio predicts chronic kidney disease: a systematic review and meta-analysis, 1998–2019
Authors: Liu Ling , Wang Yanqiu , Zhang Wanjun , Chang Weiwei , Jin Yuelong , Yao Yingshui
Published: December 2019

Background

The incidence of chronic kidney disease (CKD) increases each year, and obesity is an important risk factor for CKD. The main anthropometric indicators currently reflecting obesity are body mass index (BMI), waist circumference (WC), waist-to-hip ratio (WHR) and waist-to-height ratio (WHtR), but the rationality and merits of various indicators vary. This article aims to find whether the WHtR is a more suitable physical measurement that can predict CKD.

Methods

Pubmed, embase, the cochrane library, and web of science were systematically searched for articles published between 1998 and 2019 screening CKD through physical indicators. Two reviewers independently screened the literature according to the inclusion and exclusion criteria, extracted the data, and evaluated the quality of the methodology included in the study. Meta-analysis used the Stata 12.0 software.

Results

Nine studies were included, with a total of 202,283 subjects. Meta-analysis showed that according to the analysis of different genders in 6 studies, regardless of sex, WHtR was the area with the largest area under the curve (AUC). Except WHtR and visceral fat index (VFI) in women which showed no statistical difference, WHtR and other indicators were statistically different. In three studies without gender-based stratification, the area under the curve AUC for WHtR remained the largest, but only the difference between WHtR and BMI was statistically significant. When the Chinese population was considered as a subgroup, the area under the curve AUC for WHtR was the largest. Except for WHtR and VFI which showed no statistical difference in women, there was a statistically significant difference between WHtR and other indicators in men and women.

Conclusion

WHtR could be better prediction for CKD relative to other physical measurements. It also requires higher-quality prospective studies to verify the clinical application of WHtR.
Understanding the role of values in institutional change: the case of the energy transition
Authors: Milchram Christine , Märker Carolin , Schlör Holger , Künneke Rolf , van de Kaa Geerten
Published: December 2019

The current transition towards low-carbon energy systems does not only involve changes in technologies but is also shaped by changes in the rules and regulations (i.e., the institutions) that govern energy systems. Institutional change can be influenced by changes in core values—normative principles such as affordability, security of supply, and sustainability. Analyzing this influence, however, has been hindered by the absence of a structured framework that highlights the role of values in institutional change processes. This paper presents an interdisciplinary framework explicating how values influence institutional change in the case of the energy transition. We build on a dynamic framework for institutional change that combines the Institutional Analysis and Development (IAD) framework with the concept of social learning. This basic analytical framework is expanded by conceptualizations of values in moral philosophy, institutional economics, and social psychology. Our framework offers researchers and policy makers an analytical tool to identify how values are embedded in infrastructure and existing regulation and how values shape communities and behavior. It explains how value controversies can trigger social learning processes that eventually can result in structural change. Thus, this framework allows analyzing institutional change over time as well as comparing change patterns across spatial and temporal contexts.
Bi-allelic mutations in uncoordinated mutant number-45 myosin chaperone B are a cause for congenital myopathy
Authors: Dafsari Hormos Salimi , Kocaturk Nur Mehpare , Daimagüler Hülya-Sevcan , Brunn Anna , Dötsch Jörg , Weis Joachim , Deckert Martina , Cirak Sebahattin
Published: December 2019

Congenital myopathies (CM) form a genetically heterogeneous group of disorders characterized by perinatal muscle weakness. Here, we report an 11-year old male offspring of consanguineous parents of Lebanese origin. He presented with proximal weakness including Gower’s sign, and skeletal muscle biopsy revealed myopathic changes with core-like structures. Whole exome sequencing of this index patient lead to the discovery of a novel genetically defined CM subtype based on bi-allelic mutations in the uncoordinated mutant number-45 myosin chaperone B (UNC45B) NM_173167:c.2261G > A, p.Arg754Gln. The mutation is conserved in evolution and co-segregates within the pedigree with the phenotype, and located in the myosin binding armadillo repeat domain 3 (ARM3), and has a CADD Score of 35. On a multimeric level, UNC45B aggregates to a chain which serves as an assembly line and functions as a “template” defining the geometry, regularity, and periodicity of myosin arranged into muscle thick filaments. Our discovery is in line with the previously described myopathological phenotypes in C. elegans and in vertebrate mutants and knockdown–models. In conclusion, we here report for the first time a patient with an UNC45B mutation causing a novel genetically defined congenital myopathy disease entity.
Development and validation of an improved HPLC-UV method for simultaneous determination of lamotrigine and oxcarbazepine and its active metabolite 10,11-dihydro-10-hydroxycarbazepine in human blood plasma and comparison with an UHPLC-MS/MS method
Authors: Jin Siyao , Zhao Qing , Zhang Dongjie , Zhao Zhigang , Mei Shenghui
Published: December 2019

Lamotrigine (LTG) and oxcarbazepine (OXC) are first-line drugs for epilepsy treatment. Their large pharmacokinetics variabilities and relations between efficacy and toxicity and blood plasma concentration require routine monitoring for dose adjustment. In this study, we developed and validated a simple, accurate, and reliable method for simultaneous determination of LTG, OXC and 10,11-dihydro-10-hydroxycarbazepine (MHD) in human blood plasma by high-performance liquid chromatography-ultraviolet detection (HPLC-UV) with a simple one-step protein precipitation using methanol (1% acetic acid) and 15 min elution time under isocratic elution at 1 mL/min. Calibration range was 2.4 to 120 mg/L for LTG, OXC, and MHD. The intra-day and inter-day bias were − 8.84 to 4.18%, and the imprecision was less than 8.08% for all analytes. The internal standard (fluconazole) normalized recovery was 96.30 to 107.69% for LTG, 98.51 to 111.04% for MHD, and 95.04 to 109.86% for OXC. A total of 186 LTG samples and 25 MHD samples were used to evaluate the agreement between HPLC-UV and ultra-performance liquid chromatography-mass spectrometry (UHPLC-MS/MS) by Passing-Bablok regression and Bland-Altman plot. The mean bias and the 95% limits of agreement (95% LOA) of the two measurements were 0.575 mg/L and − 1.238 to 2.387 mg/L for LTG (n = 186) and − 1.222 mg/L and − 8.271 to 5.827 mg/L for MHD (n = 25), which indicated the UV method was comparable with the MS method for LTG and MHD analysis.
Clinical and radiographics results at 3 years of RCT with split-mouth design of submerged vs. nonsubmerged single laser-microgrooved implants in posterior areas
Authors: Guarnieri Renzo , Di Nardo Dario , Di Giorgio Gianni , Miccoli Gabriele , Testarelli Luca
Published: December 2019

Aim

To evaluate and compare radiographic crestal bone loss (CBL) and soft tissue parameters around submerged/two-stage and nonsubmerged/one-stage single implants with the same endosseous portion (body design and surface, thread design and distance) and identical intramucosal laser-microgrooved surface, after 3 years of loading.

Materials and methods

Twenty submerged/two-stage implants and 20 nonsubmerged/one-stage implants were placed randomly with a split-mouth design in the posterior areas of 20 partially edentulous patients.

Radiographic and clinical examinations were carried out at the implant placement, at the delivery of prosthetic restorations, and at each year of the follow-up period. Plaque index (PI), probing depth (PD), bleeding on probing (BOP), and gingival recession (REC) were recorded. Radiographic crestal bone levels were assessed at the mesial and distal aspect of the implant sites. In addition, the influence of the vertical keratinized tissue thickness (KTT) on CBL was investigated.

Results

At the delivery of prosthetic restorations, a statistically significant difference (P = 0.013) was found in radiographic mean CBL between submerged and nonsubmerged implants (0.15 ± 0.05 mm vs. 0.11 ± 0.04 mm). At the end of the follow-up period, no statistical difference (P = 0.741) was found in the mean CBL between submerged and nonsubmerged implants (0.27 ± 04 mm vs. 0.26 ± 0.5 mm). The changes in the soft tissues including PI, PD, BOP, and REC had no significant differences in either group. Moreover, KTT did not show a statistical correlation with CBL.

Conclusions

After 3 years of loading, no statistical difference was noted in CBL and soft tissue conditions between single submerged two-stage and nonsubmerged one-stage laser-microgrooved implants.

Trial registration

http://clinicaltrials.gov/ct2/show/NCT03674762
Emergent laparoscopic dome resection and omental suturing to the splenic parenchymal edge for a spontaneously ruptured non-parasitic large splenic cyst in a pediatric patient: a case report
Authors: Shono Kumiko , Hashimoto Yoshiko , Shono Takeshi
Published: December 2019

Background

Spontaneously ruptured large splenic cyst is a rare in children, and traditionally total or partial splenectomy has been performed for treating an emergent case. We herein present a first case with spontaneously ruptured pediatric splenic cyst treated with emergent laparoscopic dome resection with omental suturing to the parenchymal edge of the spleen.

Case presentation

A 12-year-old girl with a spontaneously ruptured large non-parasitic splenic cyst (SC) was successfully treated by emergent laparoscopic dome resection with omental suturing to the edge of the splenic parenchyma. The patient presented with acute abdominal pain and was diagnosed with a ruptured non-parasitic SC and peritonitis by contrast-enhanced computed tomography (CT). Emergent laparoscopic dome resection of the SC and omental suturing to the splenic parenchymal edge were then performed. The protruding part of the cyst wall was completely resected using an ultrasonically activated device (USAD), and the greater omentum was then sutured to the anterior edge of the splenic parenchyma under a laparoscopic view. No complications were observed during the operation. A histological examination revealed a congenital splenic cyst lined by epithelial cells. The postoperative course was uneventful, and an ultrasound scan showed no evidence of cyst recurrence at 3 years after the operation.

Conclusions

This minimally invasive laparoscopic procedure was feasible and effective for treating a ruptured large splenic cyst in an emergent pediatric patient.
Mixed methods evaluation of an employer-led, free lunch initiative in Northern Ireland
Authors: Schliemann Désirée , McKinley Michelle C. , Woodside Jayne V.
Published: December 2019

Background

The objective of this study was to evaluate the acceptance of an employer-led free lunch initiative and its effect on health, diet, and attitudes towards health and diet amongst employees in a small workplace in Northern Ireland.

Methods

This was a controlled, employer-led pilot intervention, which was evaluated through a mixed methods approach.

Results

Seventeen participants from the intervention site and 14 participants from the control site completed all assessments. Post-intervention, there was no difference in change in dietary measures between the sites, except for saturated fat intake during weekdays (IS: − 1.3% of calories, SD: 4.3; CS: 2.8% of calories, SD: 6.6; P-value < 0.05). Qualitative information was summarised to highlight employees’ expectations and experiences with the intervention.

Conclusion

This study highlights the challenges that need to be considered when implementing a free lunch initiative for staff.
Associations between normal weight central obesity and cardiovascular disease risk factors in Japanese middle-aged adults: a cross-sectional study
Authors: Shirasawa Takako , Ochiai Hirotaka , Yoshimoto Takahiko , Nagahama Satsue , Kobayashi Mariko , Ohtsu Iichiro , Sunaga Yuma , Kokaze Akatsuki
Published: December 2019

Background

Several studies have shown that normal weight central obesity (NWCO) is associated with cardiovascular disease (CVD) risk factors. However, studies conducted in the Japanese population have been very limited. Thus, the relationships between normal weight central obesity, classified using body mass index (BMI), the waist-to-height ratio (WHtR), and CVD risk factors in middle-aged Japanese adults were investigated.

Methods

The participants were Japanese adults aged 40–64 years who had undergone periodic health examinations in Japan during the period from April 2013 to March 2014. The participants were categorized into the following four groups: normal weight (BMI 18.5–24.9 kg/m2) and no central obesity (WHtR < 0.5) (NW); normal weight and central obesity (WHtR ≥ 0.5) (NWCO); obesity (BMI ≥ 25 kg/m2) and no central obesity (OB); and obesity and central obesity (OBCO). Hypertension was defined as systolic blood pressure ≥ 140 mmHg, diastolic blood pressure ≥ 90 mmHg, or taking medication for hypertension. Dyslipidemia was defined as LDL-C ≥ 140 mg/dl, HDL-C < 40 mg/dl, triglyceride ≥ 150 mg/dl, or taking medication for dyslipidemia. Diabetes was defined as fasting plasma glucose ≥ 126 mg/dl, random plasma glucose ≥ 200 mg/dl, HbA1c ≥ 6.5%, or receiving medical treatment for diabetes mellitus. A logistic regression model was used to calculate the odds ratios (ORs) and 95% confidence intervals (CIs) for hypertension, dyslipidemia, and diabetes.

Results

A total of 117,163 participants (82,487 men and 34,676 women) were analyzed. The prevalence of NWCO was 15.6% in men and 30.2% in women. With reference to NW, the ORs for hypertension (adjusted OR 1.22, 95% CI 1.17–1.27 in men, 1.23, 1.16–1.31 in women), dyslipidemia (1.81, 1.74–1.89 in men, 1.60, 1.52–1.69 in women), and diabetes (1.35, 1.25–1.46 in men, 1.60, 1.35–1.90 in women) were significantly higher in NWCO.

Conclusions

Normal weight with central obesity was associated with CVD risk factors, such as hypertension, dyslipidemia, and diabetes, compared with normal weight without central obesity, regardless of sex. It is important to focus on normal weight with central obesity for the prevention of CVD in Japanese middle-aged adults.
Factorial validity and measurement invariance of the uncertainty response scale
Authors: Lucas Casanova Mariana , Pacheco Lara S. , Costa Patrício , Lawthom Rebecca , Coimbra Joaquim L.
Published: December 2019

This study presents the adaptation of the Uncertainty Response Scale (Greco & Roger, Pers. Individ. Differ, 31:519-534, 2001) to Portuguese. This instrument was administered to a non-clinical community sample composed of 1596 students and professionals, allowing a thorough validity and invariance analysis by randomly dividing participants into three subsamples to perform: an exploratory factor analysis (sample one: N = 512); a preliminary confirmatory factor analysis to identify the final solution for the scale (sample two: N = 543); and the confirmatory factor analysis (sample three: N = 541). Samples two and three were also used for multi-group analysis to assess measurement invariance, invariance across gender, sociocultural levels, and students versus active professionals. Results showed the scale reflects the original factorial structure, as well as good internal consistency and overall good psychometric qualities. Invariance results across groups reached structural invariance which provides a confident invariance measurement for this scale, while invariance across gender and sociocultural levels reached metric invariance. Accordingly, differences between these groups were explored, by comparing means with multi-group analysis to establish the scale’s sensitivity toward social vulnerability, by demonstrating the existence of statistically significant differences regarding gender and sociocultural levels on how individuals cope with uncertainty, specifically in terms of emotional strategies, as a self-defeating strategy. Thus, females scored higher on emotional uncertainty, as well as low sociocultural levels, compared with higher ones. Therefore, it is proposed that this scale could be a sound alternative to explore strategies for coping with uncertainty, when considering social, economic, or other environmental circumstances that may affect them.
Successful case of complex atrial flutter occurring in a patient with congenitally corrected transposition of the great arteries, aberrant left atrial appendage, and situs inversus
Authors: Hu Feng , Liang Erpeng , Zheng Lihui , Ding Ligang
Published: December 2019

Background

Congenitally corrected transposition of great arteries (ccTGA) is a rare congenital cardiac defect with atrioventricular and ventriculoarterial discordance which leads to heart failure and limits patients’ lifespan. The extremely aberrant cardiac structure makes electrophysiological procedure and radiofrequency ablation very difficult to be performed in such patients. Until now, there were only sporadical cases that have reported the successful ablation of atrial flutter in ccTGA patients.

Case presentation

We report a case of a 36-year-old male who was diagnosed with dextrocardia, atrial septal defect and congenitally corrected transposition of great arteries (ccTGA) at a young age and received atrial septal defect repair and morphological tricuspid valve plasty in 2014. As for reasons of heart failure and atrial flutter, he frequently suffered from progressively worsening dyspnea and recurrent episodes of palpitations. Cardiac anatomic imaging reconstruction before electrophysiological test revealed an unusually huge left atrial appendage in this patient. After high-density mapping of both right atrium and left atrium, activation mapping showed reentry circuit loops were located in left atrium. Successful ablation strategy was performed under the guidance of high-density mapping and entrainment.

Conclusion

This is a clinical case showing high-density mapping and successful ablation of a complex dual-loop atrial flutter in a patient with ccTGA and aberrant left atrial appendage. The successful procedure corroborates clinical utility of high-density mapping approach in the treatment of the patients with complex congenital heart disease accompanied by rapid arrhythmia, can be simpler, safer and more effective.
Thyroid dysfunction in patients with suspected or documented supraventricular tachyarrhythmia
Authors: Gwag Hye Bin , Jun Ji Eun , Park Youngjun , Lee Seong Soo , Park Seung-Jung , Kim June Soo , Park Kyoung-Min , On Young Keun
Published: December 2019

Background and objectives

The association between thyroid dysfunction and supraventricular tachyarrhythmia (SVT) other than atrial fibrillation (AF) is not well elucidated. We hypothesized that patients with suspected or documented SVT have undetected thyroid dysfunction more frequently than general population.

Subjects and methods

Patients with symptoms suggestive of SVT other than AF who were planned to undergo an EP study at our center were eligible for this study. Patients with known thyroid disease, medications affecting thyroid function test (TFT) or atrial fibrillation were excluded. The primary outcome was prevalence of TFT abnormalities in the study population. The secondary outcome was prevalence of clinically significant arrhythmia during the EP study.

Results

A total of 533 patients were analyzed. Patients were divided into the two groups according to TFT results. Fifty-six (10.5%) patients had abnormal TFT results (thyroid dysfunction group), while 477 (89.5%) had normal thyroid function (euthyroidism group). The overall prevalence of thyroid dysfunction was 10.5%. The thyroid dysfunction group showed a tendency for higher induction of clinically significant SVT than the euthyroidism group. However, thyroid dysfunction was not an independent predictor of clinically significant SVT during EP study.

Conclusion

In this study, patients with suspected SVT who were planned for EP study had a relatively high incidence of thyroid dysfunction. Clinically significant arrhythmia tended to be induced more frequently in the thyroid dysfunction group than in the euthyroidism group. Further investigations are needed to verify the clinical implications of TFT in patients with SVT other than AF.
The influence of computer-assisted surgery experience on the accuracy and precision of the postoperative mechanical axis during computer-assisted lateral closing-wedge high tibial osteotomy
Authors: Lee Hyun Woo , Song Sang Jun , Bae Dae Kyung , Park Cheol Hee
Published: December 2019

Background

There is debate regarding the influence of a surgeon’s experience with computer-assisted surgery (CAS) on the postoperative mechanical axis (MA) in CAS-high tibial osteotomy. The purpose of the present study was to compare radiographic results between early and late cohorts of a consecutive series of patients to assess the influence of CAS experience on accuracy and precision of the postoperative MA during CAS lateral closing-wedge high tibial osteotomy (LCWHTO).

Materials and methods

Results from 140 CAS-LCWHTO operations were retrospectively reviewed. The first 70 cases, performed during the learning curve period for CAS between 2005 and 2009, were considered to be the “early cohort.” The subsequent 70 cases, performed with greater CAS experience after the completion of the learning curve between 2009 and 2014, were considered to be the “late cohort.” The target postoperative MA angle was valgus 3°. Pre- and postoperative MA angles were evaluated by navigation and radiographs. The proportion of postoperative MA inliers (≤ target angle ±3°) was investigated radiographically. The correlation between the navigation and radiographic measurements was analyzed.

Results

The average postosteotomy MA angle on navigation was 3.4° in both cohorts. The average postoperative MA angle on radiographs was 1.0° in the early cohort and 2.2° in the late cohort (P = 0.003). Radiographically, the proportion of postoperative MA inliers was greater in the late cohort than in the early cohort (early versus late, 71.4% versus 90%; P = 0.011). The pre- and postoperative correlation between navigation and radiographic measurements was also stronger in the late cohort (early versus late; preoperative r = 0.558 versus 0.663; postoperative r = 0.310 versus 0.376).

Conclusions

Greater experience with CAS increased the accuracy and precision of postoperative MA alignment as well as the correlation between navigation and radiographic measurements. Caution should be taken during registration procedures to achieve accurate alignment correction in CAS-LCWHTO.
The correlation between endometrial thickness and pregnancy outcomes in fresh ART cycles with different age groups: a retrospective study
Authors: Eftekhar Maryam , Mehrjardi Sara Zare , Molaei Behnaz , Taheri Fatemeh , Mangoli Esmat
Published: December 2019

Background

In assisted reproductive technology (ART) cycles in addition to embryo quality, the receptivity of the endometrium plays the main role in clinical outcomes. Endometrial receptivity is necessary to implantation of an embryo, and ultrasound has been established as an appreciated, simple, and non-invasive technique in the evaluation of the endometrial preparation before embryo transfer in fresh in vitro fertilization (IVF) cycles. Debate on the predictive value measuring endometrial thickness before administering human chorionic gonadotropin (HCG) for ovulation triggering in ART is ongoing. In order to explore the impact of endometrial thickness on triggering day on ongoing pregnancy rate (OPR) in ART cycles, we retrospectively analyzed data from 1000 patients undergoing IVF/ICSI cycles.

Results

The data showed pregnancy rate was increased in the endometrial thickness of 8 mm to 11 mm then decreased, and in endometrial thickness (Ent) > 14 mm, pregnancy rate was zero. There were significant differences in endometrial thickness and pregnancy outcomes between different age groups. The pregnancy rate was higher (32%) in 23–30 years old women, and the range of Ent in this group was 6–12 mm. Also, the data showed a positive correlation between Ent with AMH and estradiol levels and the number of COC and MII oocytes and a negative correlation between female age with Ent.

Conclusion

The result showed that Ent on hCG administration day is associated with pregnancy outcomes in fresh IVF/ICSI cycles with different age groups, although some of the clinical parameters may have an effect on Ent. Large studies are needed to make a definitive conclusion.
MDCT angiographic findings of various congenital pulmonary artery anomalies in pediatric patients
Authors: Harraz Mohamed M. , Abouissa Ahmed H. , Saleh Hala A. , Attas Khalid A. , Al-Yamani Samira M. , Alsulami Ganadiel , Refai Mohamed M.
Published: December 2019

Background

Congenital pulmonary artery anomalies are variable and need proper diagnosis and treatment. CT angiography with multiplanar reconstruction has the main role in the assessment of these anomalies and this non-invasive method should be the method of choice for preoperative planning and postoperative follow up.

The aim of the study is to assess the value of MDCT in the detection of pulmonary arteries anomalies in the pediatric population with complex congenital heart disease in conjunction with echocardiography as an alternative to conventional angiography and to determine the superiority of MDCT in the assessment of other abnormalities such as airway anomalies.

Results

In our retrospective study, 52 patients (28 male and 24 females, aged 1 day to 4 years: mean age 2 years) were examined with contrast-enhanced CT. CT examinations were done using a 128-section CT scanner (Siemens Somatom Definition AS) using non-ionic iodinated contrast media. 2D and 3D reconstructions were performed. The correlation was made with echocardiograms. All imaging studies were reviewed. The echo was done to all patients. Surgery and/or catheter angiography performed to all patients, their findings were reviewed and compared to CTA findings. Other abnormalities such as congenital airway anomalies are detected using axial MDCT images and reconstructed imaging techniques.

MDCT was accurate in revealing pulmonary artery anomalies. The commonest pulmonary artery anomaly was atresia, stenosis then hypoplasia. These anomalies may be isolated or associated with other congenital heart diseases. In the current study, MDCT could diagnose all cases of pulmonary arterial anomalies with 96% sensitivity, 100% specificity, 98% accuracy, 100% positive, and 94% negative predictive values. CT scans provide accurate information to assess complex spatial relationships of vascular airway compression frequently associated with CHD in the pediatric population.

Conclusion

MDCT scanner can be an alternative to diagnostic conventional angiography for the non-invasive assessment of the pulmonary artery. Higher quality multiplanar and 3D reconstruction achieved by the MDCT scanners offer a rapid, reliable and non-invasive technique that can be used for the evaluation and preoperative assessment of thoracic vascular and extra-vascular anatomy in infants and children with suspected congenital heart disease. CT technologies are constantly developing collaboration between radiologists, pediatric cardiologists, and anesthesiologists, which is essential for improving CT performance.
Seismic characterization of geothermal sedimentary reservoirs: A field example from the Copenhagen area, Denmark
Authors: Bredesen Kenneth , Dalgaard Esben , Mathiesen Anders , Rasmussen Rasmus , Balling Niels
Published: December 2019

We have seismically characterized a Triassic-Jurassic deep geothermal sandstone reservoir north of Copenhagen, onshore Denmark. A suite of regional geophysical measurements, including prestack seismic data and well logs, was integrated with geologic information to obtain facies and reservoir property predictions in a Bayesian framework. The applied workflow combined a facies-dependent calibrated rock-physics model with a simultaneous amplitude-variation-with-offset seismic inversion. The results suggest that certain sandstone distributions are potential aquifers within the target interval, which appear reasonable based on the geologic properties. However, prediction accuracy suffers from a restricted data foundation and should, therefore, only be considered as an indicator of potential aquifers. Despite these issues, the results demonstrate new possibilities for future seismic reservoir characterization and rock-physics modeling for exploration purposes, derisking, and the exploitation of geothermal energy as a green and sustainable energy resource.
Inability to Mask Ventilate During Myringotomy Tube Replacement: A Case Report
Authors: Wong Ashley V. , Balakrishnan Karthik , Nemergut Michael E.
Published: December 2019

We present the case of a 3-year-old female with multiple congenital anomalies, including postprandial otorrhea, who developed the inability to be mask ventilated secondary to a combination of velopharyngeal insufficiency and tympanic membrane perforation. When applied by mask, positive pressure ventilation was observed to preferentially escape the patient’s left ear, resulting in significant air leak, insufficient tidal volumes, hypoventilation, and severe hypoxemia. The problem was remedied by the insertion of a finger into the patient’s external auditory meatus, which controlled the air leak until the surgical team could provide definitive surgical correction of the velopharyngeal insufficiency and nasopharyngeal reflux.
Indigenous Feminist Notes on Embodying Alliance against Settler Colonialism
Authors: Arvin Maile
Published: December 2019

How can we enact meaningful forms of solidarity across Indigenous and non-Indigenous communities? This essay, which focuses specifically on the context of settler colonialism in Hawaiʻi, examines existing or potential alliances between Indigenous feminisms and transnational feminisms. Written from a Kanaka Maoli (Native Hawaiian) feminist perspective, the essay looks to the foundational work of Kanaka Maoli scholar-activist Haunani-Kay Trask as a too often overlooked theorist of settler colonialism writ broadly. The essay also looks more specifically at Trask’s theorizing of Asian settler colonialism in the Hawaiʻi context, in relation to contemporary examples of conflicts between Native Hawaiians and the state, as well as Native Hawaiian activists and white feminists. Overall, the essay questions how reframing Asian settler colonialism in more concerted conversation with Indigenous feminisms and transnational feminisms might provide space to move our practices of solidarity against settler colonialism, imperialism, nativism, militarization, and environmental destruction into a generative space for Kānaka Maoli and non-Indigenous peoples alike.
Peace and the Barrel of the Gun in the Internationalist Women’s Movement, 1945–49
Authors: Armstrong Elisabeth
Published: December 2019

In 1949, at a conference instigated by the Women’s International Democratic Federation (WIDF) held in Beijing, China, the Asian Women’s Conference solidified an anticolonial, antifascist, and antiracist theory for organizing women transnationally. This transnational feminist praxis drew its movement demands and strategies from the masses of women in anticolonial movements, both rural and urban poor women. It also framed a two-fold theory of women’s organizing: it delineated one platform for women fighting imperialism within colonized countries, and another platform for women fighting imperialism within aggressor nations. This transnational feminism supported an explicitly pro-socialist vision for the future.
Characterization of spinal cord damage based on automatic video analysis of froglet swimming
Authors: De Vidts Sebastián , Méndez-Olivos Emilio , Palacios Miriam , Larraín Juan , Mery Domingo
Published: December 2019

Xenopus laevis frogs are a widely used organism to study aspects of modern biology (Harland and Grainger, 2011). Its central nervous system is particularly interesting, because in certain stages of metamorphosis the spinal cord can regenerate after injury and recover swimming. With this in mind, automatic gait analysis could help evaluate the regenerative performance by means of a method that automatically and quantitatively establishes the degree in froglets’ limb movement. Here, we present an algorithm that characterizes spinal cord damage in froglets. The proposed method tracks the position of the limbs throughout videos and extracts kinematic features, which posteriorly serve to differentiate froglets with different levels of damage to the spinal cord. The detection algorithm and kinematic features chosen were validated in a pattern recognition experiment in which 90 videos (divided equally in three classes: uninjured, hemisected and transected) were classified. We conclude that our system is effective in the characterization of damage to the spinal cord through video analysis of a swimming froglet with a 97% accuracy. These results potentially validate this methodology to automatically compare the recovery of spinal cord function after different treatments without the need to manually process videos. In addition, the procedure could be used to measure the kinematics and behavioral response of froglets to different experimental conditions such as nutritional state, stress, genetic background and age.