bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimization of an Ischemic Retinopathy Mouse Model and the Consequences of Hypoxia in a Time-Dependent Manner
Authors: Bosnyak Inez , Farkas Nelli , Molitor Dorottya , Meresz Balazs , Patko Evelin , Atlasz Tamas , Vaczy Alexandra , Reglodi Dora
Published: July 2024

The retina is one of the highest metabolically active tissues with a high oxygen consumption, so insufficient blood supply leads to visual impairment. The incidence of related conditions is increasing; however, no effective treatment without side effects is available. Furthermore, the pathomechanism of these diseases is not fully understood. Our aim was to develop an optimal ischemic retinopathy mouse model to investigate the retinal damage in a time-dependent manner. Retinal ischemia was induced by bilateral common carotid artery occlusion (BCCAO) for 10, 13, 15 or 20 min, or by right permanent unilateral common carotid artery occlusion (UCCAO). Optical coherence tomography was used to follow the changes in retinal thickness 3, 7, 14, 21 and 28 days after surgery. The number of ganglion cells was evaluated in the central and peripheral regions on whole-mount retina preparations. Expression of glial fibrillary acidic protein (GFAP) was analyzed with immunohistochemistry and Western blot. Retinal degeneration and ganglion cell loss was observed in multiple groups. Our results suggest that the 20 min BCCAO is a good model to investigate the consequences of ischemia and reperfusion in the retina in a time-dependent manner, while the UCCAO causes more severe damage in a short time, so it can be used for testing new drugs.
Gas-Sensing Performance of Metal Oxide Heterojunction Materials for SF6 Decomposition Gases: A DFT Study
Authors: Zeng Tingting , Ma Donglin , Gui Yingang
Published: July 2024

The online monitoring of GIS equipment can be realized through detecting SF6 decomposition gasses. Metal oxide heterojunctions are widely used as gas-sensing materials. In this study, the structural and electrical properties of In2O3-ZnO and TiO2-ZnO heterojunctions were analyzed based on density functional theory calculations. After heterojunction structural optimization, the electrical conductivity of these two heterojunctions was enhanced compared to each intrinsic model, and the electrical conductivity is ranked as follows: In2O3-ZnO heterojunction > TiO2-ZnO heterojunction. The gas-sensing response of these two heterojunctions to four SF6 decomposition gasses, H2S, SO2, SOF2, and SO2F2, was investigated. For gas adsorption systems, the adsorption energy, charge transfer, density of states, charge difference density, and frontier molecular orbitals were calculated to analyze the adsorption and gas-sensing performance. For gas adsorption on the In2O3-ZnO heterojunction surface, the induced conductivity changes are in the following order: H2S > SO2F2 > SOF2 > SO2. For gas adsorption on the TiO2-ZnO heterojunction surface, H2S and SOF2 increase conductivity, and SO2 and SO2F2 decrease conductivity.
A Scaled Proteomic Discovery Study for Prostate Cancer Diagnostic Markers Using ProteographTM and Trapped Ion Mobility Mass Spectrometry
Authors: Chang Matthew E. K. , Lange Jane , Cartier Jessie May , Moore Travis W. , Soriano Sophia M. , Albracht Brenna , Krawitzky Michael , Guturu Harendra , Alavi Amir , Stukalov Alexey , Zhou Xiaoyuan , Elgierari Eltaher M. , Chu Jessica , Benz Ryan , Cuevas Juan C. , Ferdosi Shadi , Hornburg Daniel , Farokhzad Omid , Siddiqui Asim , Batzoglou Serafim , Leach Robin J. , Liss Michael A. , Kopp Ryan P. , Flory Mark R.
Published: July 2024

There is a significant unmet need for clinical reflex tests that increase the specificity of prostate-specific antigen blood testing, the longstanding but imperfect tool for prostate cancer diagnosis. Towards this endpoint, we present the results from a discovery study that identifies new prostate-specific antigen reflex markers in a large-scale patient serum cohort using differentiating technologies for deep proteomic interrogation. We detect known prostate cancer blood markers as well as novel candidates. Through bioinformatic pathway enrichment and network analysis, we reveal associations of differentially abundant proteins with cytoskeletal, metabolic, and ribosomal activities, all of which have been previously associated with prostate cancer progression. Additionally, optimized machine learning classifier analysis reveals proteomic signatures capable of detecting the disease prior to biopsy, performing on par with an accepted clinical risk calculator benchmark.
Development of Non-Invasive miRNA Markers for Assessing the Quality of Human Induced Pluripotent Stem Cell-Derived Retinal Organoids
Authors: Park Hyo Song , Bang Ji-Hong , Jung Wook Hyun , Yang Jin Young , Shin Hee Jeong , Son Ji-Hye , Han Jung Woo , Lee Si Hyung , Chung Kyung Hwun , Kim Kyunggon , Chang Hun Soo , Park Tae Kwann
Published: July 2024

Human retinal organoids (ROs) have emerged as valuable tools for studying retinal development, modeling human retinal diseases, and screening drugs. However, their application is limited primarily due to time-intensive generation, high costs, and low reproducibility. Quality assessment of RO differentiation is crucial for their application in research. However, traditional methods such as morphological evaluation and immunohistochemical analysis have limitations due to their lack of precision and invasiveness, respectively. This study aims to identify non-invasive biomarkers for RO differentiation quality using exosomal microRNAs (miRNAs), which are known to reflect cell-specific functions and development in the retina. We differentiated ROs from human induced pluripotent stem cells (hiPSCs) and classified them into ‘superior’ and ‘inferior’ groups based on morphological and immunohistochemical criteria. Exosomes from the conditioned media were isolated and analyzed for miRNA content. Our findings revealed distinct miRNA profiles between superior and inferior ROs, with superior ROs exhibiting higher miRNA diversity and specifically up- or down-regulated miRNAs. Gene ontology and pathway enrichment analyses indicated that the target genes of these miRNAs are involved in neuron proliferation and differentiation. The study suggests the potential of exosomal hsa-miR-654-3p and hsa-miR-451a as non-invasive biomarkers for real-time monitoring of RO quality, facilitating the development of standardized, efficient, and cost-effective culture methods.
Promising Experimental Treatment in Animal Models and Human Studies of Interstitial Cystitis/Bladder Pain Syndrome
Authors: Hu Ju-Chuan , Tzeng Hong-Tai , Lee Wei-Chia , Li Jian-Ri , Chuang Yao-Chi
Published: July 2024

Interstitial cystitis/bladder pain Syndrome (IC/BPS) remains a mysterious and intricate urological disorder, presenting significant challenges to healthcare providers. Traditional guidelines for IC/BPS follow a hierarchical model based on symptom severity, advocating for conservative interventions as the initial step, followed by oral pharmacotherapy, intravesical treatments, and, in refractory cases, invasive surgical procedures. This approach embraces a multi-tiered strategy. However, the evolving understanding that IC/BPS represents a paroxysmal chronic pain syndrome, often involving extravesical manifestations and different subtypes, calls for a departure from this uniform approach. This review provides insights into recent advancements in experimental strategies in animal models and human studies. The identified therapeutic approaches fall into four categories: (i) anti-inflammation and anti-angiogenesis using monoclonal antibodies or immune modulation, (ii) regenerative medicine, including stem cell therapy, platelet-rich plasma, and low-intensity extracorporeal shock wave therapy, (iii) drug delivery systems leveraging nanotechnology, and (iv) drug delivery systems assisted by energy devices. Future investigations will require a broader range of animal models, studies on human bladder tissues, and well-designed clinical trials to establish the efficacy and safety of these therapeutic interventions.
Cellular Effects of Enterobacteriaceae Polysaccharide Colanic Acid
Authors: Tsvetikova Sofia A. , Zabavkina Alina A. , Ivankova Olesia , Koshel Elena I.
Published: July 2024

Colanic acid (CA) is an exopolysaccharide found in Enterobacteriaceae. Recently, its ability to stimulate physical activity in mice and to prolong the lifespan of invertebrates has been described. In the current work, we use standard MTT assay, fluorescence microscopy, and flow cytometry to describe CA action on several cell lines of different origins. We observed slight antiproliferative activity against colorectal cancer (HCT-116), neuroblastoma (IMR-32), and myoblast (C2C12) cell lines at a concentration of 256 μg/mL, while other cell lines of non-cancerous origin (Vero, HPF) did not show any decrease in the MTT assay. In all cell lines, we observed a rearrangement of mitochondria localization using fluorescence microscopy. CA induces cell differentiation in the myoblast cell line (C2C12) at concentrations of 50–200 μg/mL. Briefly, we observed that the number of apoptotic cells increased and the metabolic activity in the MTT assay decreased, which was accompanied by changes in cell morphology, the quantity of ROS, and the potential of the mitochondrial membrane. Taken together, these results indicate that CA is specific in cytotoxicity to cell lines of different origins and can impact mitochondria and differentiation, consistent with its potential geroprotective function.
Hemodynamic Evaluation of Coronary Artery Lesions after Kawasaki Disease: Comparison of Fractional Flow Reserve during Cardiac Catheterization with Myocardial Flow Reserve during 13N-Ammonia PET
Authors: Watanabe Makoto , Fukazawa Ryuji , Kiriyama Tomonari , Imai Shogo , Matsui Ryosuke , Shimada Kanae , Hashimoto Yoshiaki , Hashimoto Koji , Abe Masanori , Kamisago Mitsuhiro , Itoh Yasuhiko
Published: July 2024

Coronary artery lesions (CALs) after Kawasaki disease present complex coronary hemodynamics. We investigated the relationship between coronary fractional flow reserve (FFR), myocardial flow reserve (MFR), and myocardial blood flow volume fraction (MBF) and their clinical usefulness in CALs after Kawasaki disease. Nineteen patients (18 men, 1 woman) who underwent cardiac catheterization and 13N-ammonia positron emission tomography, with 24 coronary artery branches, were included. Five branches had inconsistent FFR and MFR values, two had normal FFR but abnormal MFR, and three had abnormal FFR and normal MFR. The abnormal MFR group had significantly higher MBF at rest than the normal group (0.86 ± 0.13 vs. 1.08 ± 0.09, p = 0.001). The abnormal FFR group had significantly lower MBF at adenosine loading than the normal group (2.23 ± 0.23 vs. 1.88 ± 0.29, p = 0.021). The three branches with abnormal FFR only had stenotic lesions, but the MFR may have been normal because blood was supplied by collateral vessels. Combining FFR, MFR, and MBF will enable a more accurate assessment of peripheral coronary circulation and stenotic lesions in CALs and help determine treatment strategy and timing of intervention.
Adverse Events with Hypoglossal Nerve Stimulation in the Treatment of Obstructive Sleep Apnea—A Systematic Review of Clinical Trials and Real-World Data
Authors: Wollny Mathias , Heiser Clemens , Sommer Ulrich , Schöbel Christoph , Braun Marcel
Published: July 2024

Background/Objectives: Hypoglossal nerve stimulation (HNS) emerged as an alternative treatment for patients with obstructive sleep apnea (OSA) a decade ago. Long-term clinical trials and real-world data show that HNS treatment provides significant and sustained improvements in both OSA disease control and quality-of-life measures over time. Given the nature of HNS treatment, with the requirement of using an implantable neurostimulation system, patient safety is a critical domain in the assessment of this technology. The objective of this review was to evaluate adverse events (AEs) and complications with HNS therapy in a systematic review of published evidence. Methods: Medline, Cochrane, and Web of Science were systematically searched to identify randomized controlled and real-world observational studies reporting relevant outcomes with HNS therapy for treatment of OSA that included procedure-, device-, and treatment-related AEs. Results: Out of 418 articles screened, 27 were reviewed for eligibility, and 17 studies, the majority found to have low-to-moderate risk of bias, with data on 1962 patients were included for further analysis. Across included studies, reporting of AEs was heterogeneous with regard to the classifications used and the extent of reporting. Over an average follow-up duration of 17.5 ± 16.9 months, the pooled mortality rate was 0.01% (95% CI = 0.0 to 0.2%), with all reported deaths being unrelated to HNS treatment. The HNS system survival probability over the follow-up time of 60 months was 0.9834 (95% CI = 0.9768 to 0.9882), with infections and request for removal by patients being the most common indications. The pooled surgical revision rate was 0.08% (95% CI 0.0 to 0.2%). Most reported treatment-related side effects were transient stimulation-related discomfort (0.08%, 95% CI = 0.0 to 0.2%) and tongue abrasions (0.07%, 95% CI = 0.0 to 0.2%). Based on the systematic review, a standardized set of endpoints was defined, aiming to harmonize safety data relevant to HNS therapy. Conclusions: In this systematic review, HNS therapy for treatment of OSA is associated with a positive patient safety profile. AEs occur mainly at device implantation and during the treatment acclimatization period. Due to a lack of available evidence, partially implantable HNS systems are underrepresented in this review, which limits the generalizability of the results. Significant heterogeneity was found for adverse event reporting. A framework for reporting HNS outcomes that includes AEs and side effects is proposed to facilitate comparability of the reported data.
Impact of COVID-19 Disease on the Development of Osteomyelitis of Jaws: A Systematic Review
Authors: Vardas Emmanouil , Adamo Daniela , Canfora Federica , Kouri Maria , Delli Konstantina , Mignogna Michele Davide , Nikitakis Nikolaos
Published: July 2024

Background/Objectives: Osteomyelitis is characterized by an inflammatory process affecting both bone and bone marrow, leading to cell death and the formation of bone sequestrum. Recent literature from the past five years has documented instances of osteomyelitis following infections of SARS-CoV-2. This systematic review explores the link between osteomyelitis of the jaw (OMJ) and COVID-19 infections. Methods: This review adhered to the PRISMA guidelines, systematically analyzing literature from 2020 to 2024 sourced from databases including Medline, Embase, Scopus, and Web of Science. PROSPERO ID: CRD42024526257. Results: The review selected 42 articles, detailing 201 cases of osteomyelitis of the jaw related to COVID-19 (COMJ). The demographic breakdown included 195 male (74.4%) and 67 female patients (25.6%), with a median age of 52.7 years, ranging from 24 to 71 years. A significant portion of COMJ patients (41.5%) were hospitalized due to COVID-19, and 58.5% received corticosteroid therapy. Diabetes mellitus was a common comorbidity among COMJ patients (65.1%). Most cases involved maxilla (182 cases; 90.5%), with nearly half showing sinus involvement (49.4%). The mandible was affected in 19 cases (9.5%). Mucormycosis and aspergillosis emerged as the predominant fungal infections, identified in 103 (51.2%) and 50 (24.9%) cases, respectively. Conclusions: Individuals with pre-existing health conditions such as diabetes mellitus who have been treated for COVID-19 are at an increased risk of developing OMJ, particularly maxillary fungal osteomyelitis. COMJ poses a significant diagnostic and therapeutic challenge for dental and maxillofacial professionals, who are often the first to encounter these cases.
Exploring Type IIIb Endoleaks: A Literature Review to Identify Possible Physical Mechanisms and Implications
Authors: Aras Tuna , Tayeh Mahmoud , Aswad Adel , Sharkawy Mohamed , Majd Payman
Published: July 2024

Endoleaks are common complications following endovascular aneurysm repair (EVAR). They can be classified into low-pressure and high-pressure endoleaks. High-pressure endoleaks, which include Type I and Type III endoleaks, pose a significant risk of rupture and require urgent treatment. The aim of our study is to review published case reports and case series to assess the impact of Type IIIb endoleaks in EVAR and to identify possible mechanisms contributing to these endoleaks. This review targeted case reports and case series published between January 1998 and December 2022. A total of 62 case reports and case series were identified, encompassing 156 patients with Type IIIb endoleaks. Data collection was performed by three consultants who thoroughly discussed each report before registering it into an analyzable data set. Our analysis revealed that, beyond material imperfections, certain endograft configurations or conformations, endograft redundancy, and the physical forces acting on the grafts may lead to increased stress on specific parts of the endografts, potentially exceeding their design limits. Factors contributing to redundancy and unfavorable conformation of the endograft include secondary interventions for any cause (such as other types of endoleaks), EVAR performed outside the instructions for use (IFUs), endograft migrations, or larger initial aneurysm diameter.
Stochastic Optimization of Onboard Photovoltaic Hybrid Power System Considering Environmental Uncertainties
Authors: Zhu Jianyun , Chen Li
Published: July 2024

Environmental uncertainties present a significant challenge in the design of onboard photovoltaic hybrid power systems (PV-HPS), a pivotal decarbonization technology garnering widespread attention in the shipping industry. Neglecting environmental uncertainties associated with photovoltaic (PV) output and hull resistance can lead to suboptimal solutions. To address this issue, this paper proposes a stochastic optimization method for PV-HPS, aiming to minimize greenhouse gas (GHG) emissions and lifecycle costs. Copula functions are employed to establish joint distributions of uncertainties in solar irradiance, ambient temperature, significant wave height, and wave period. Monte Carlo simulation, the bi-bin method, and the multi-objective particle swarm optimization (MOPSO) algorithm are utilized for scenario generation, scenario reduction, and design space exploration. The efficacy of the proposed method is demonstrated through a case study involving an unmanned ship. Additionally, deterministic optimization and two partial stochastic optimizations are conducted to underscore the importance of simultaneously considering environmental uncertainties related to power sources and hull resistance. The results affirm the proposed approach’s capability to reduce GHG emissions and lifecycle costs. A sensitivity analysis of bin number is performed to investigate the tradeoff between optimality and computation time.
A Countermeasure Strategy against Peramine Developed by Chilesia rudis in the Endophyte–Ryegrass–Herbivore Model
Authors: Chacón-Fuentes Manuel , Martínez-Cisterna Daniel , Lizama Marcelo , Asencio-Cancino Valeria , Matamala Ignacio , Bardehle Leonardo
Published: July 2024

Exploitation of the symbiotic relationship between endophytic fungi and ryegrass is a crucial technique for reducing the incidence of insect pests. This is primarily due to the production of alkaloids, such as peramine, by the fungi. This alkaloid has been reported as both a deterrent and toxic to a variety of insects. However, insects have developed various strategies to counteract plant defenses. One of the most studied methods is their ability to sequester toxic compounds from plants. In this study, we examined the feeding preferences and adaptation to peramine in Chilesia rudis, a native Chilean larva. Using a no-choice assay, we assessed larval feeding preferences and mass gain on seven experimental lines and two commercial cultivars of endophyte-infected and non-infected ryegrass. Pupal development time and adult performance were evaluated post-assay. Additionally, we measured peramine content in larval carcasses, feces, and ryegrass leaves. Jumbo was the most preferred cultivar with 32 mm2 of leaf tissues consumed. The longest pupal development time was observed in L161 and ALTO AR1, both at 28 days. Wing length in adults was greatest in the Jumbo and L163 cultivars, measuring 1.25 cm and 1.32 cm, respectively. Peramine concentrations were detected in the bodies of C. rudis. In conclusion, this larva can adapt to endophyte-infected ryegrass and develop counter-adaptation mechanisms to mitigate the effects of peramine.
Comparative Analysis of Hulless Barley Transcriptomes to Regulatory Effects of Phosphorous Deficiency
Authors: An Likun , Wang Ziao , Cui Yongmei , Bai Yixiong , Yao Youhua , Yao Xiaohua , Wu Kunlun
Published: July 2024

Hulless barley is a cold-resistant crop widely planted in the northwest plateau of China. It is also the main food crop in this region. Phosphorus (P), as one of the important essential nutrient elements, regulates plant growth and defense. This study aimed to analyze the development and related molecular mechanisms of hulless barley under P deficiency and explore the regulatory genes so as to provide a basis for subsequent molecular breeding research. Transcriptome analysis was performed on the root and leaf samples of hulless barley cultured with different concentrations of KH2PO4 (1 mM and 10 μM) Hoagland solution. A total of 46,439 genes were finally obtained by the combined analysis of leaf and root samples. Among them, 325 and 453 genes had more than twofold differences in expression. These differentially expressed genes (DEGs) mainly participated in the abiotic stress biosynthetic process through Gene Ontology prediction. Moreover, the Kyoto Encyclopedia of Genes and Genomes showed that DEGs were mainly involved in photosynthesis, plant hormone signal transduction, glycolysis, phenylpropanoid biosynthesis, and synthesis of metabolites. These pathways also appeared in other abiotic stresses. Plants initiated multiple hormone synergistic regulatory mechanisms to maintain growth under P-deficient conditions. Transcription factors (TFs) also proved these predictions. The enrichment of ARR-B TFs, which positively regulated the phosphorelay-mediated cytokinin signal transduction, and some other TFs (AP2, GRAS, and ARF) was related to plant hormone regulation. Some DEGs showed different values in their FPKM (fragment per kilobase of transcript per million mapped reads), but the expression trends of genes responding to stress and phosphorylation remained highly consistent. Therefore, in the case of P deficiency, the first response of plants was the expression of stress-related genes. The effects of this stress on plant metabolites need to be further studied to improve the relevant regulatory mechanisms so as to further understand the importance of P in the development and stress resistance of hulless barley.
The Pigment World: Life’s Origins as Photon-Dissipating Pigments
Authors: Michaelian Karo
Published: July 2024

Many of the fundamental molecules of life share extraordinary pigment-like optical properties in the long-wavelength UV-C spectral region. These include strong photon absorption and rapid (sub-pico-second) dissipation of the induced electronic excitation energy into heat through peaked conical intersections. These properties have been attributed to a “natural selection” of molecules resistant to the dangerous UV-C light incident on Earth’s surface during the Archean. In contrast, the “thermodynamic dissipation theory for the origin of life” argues that, far from being detrimental, UV-C light was, in fact, the thermodynamic potential driving the dissipative structuring of life at its origin. The optical properties were thus the thermodynamic “design goals” of microscopic dissipative structuring of organic UV-C pigments, today known as the “fundamental molecules of life”, from common precursors under this light. This “UV-C Pigment World” evolved towards greater solar photon dissipation through more complex dissipative structuring pathways, eventually producing visible pigments to dissipate less energetic, but higher intensity, visible photons up to wavelengths of the “red edge”. The propagation and dispersal of organic pigments, catalyzed by animals, and their coupling with abiotic dissipative processes, such as the water cycle, culminated in the apex photon dissipative structure, today’s biosphere.
Comment on Aşır et al. Investigation of Vitamin D Levels in Men with Suspected Infertility. Life 2024, 14, 273
Authors: Abid Hiba , Mehmood Kainat , Abid Areeba , Abid Eisha
Published: July 2024

We have reviewed the article “Investigation of Vitamin D Levels in Men with Suspected Infertility” by Fırat Aşır [...]
A Comparative Study on Force-Fields for Interstitial Diffusion in α-Zr and Zr Alloys
Authors: Li Jing , Shi Tan , Zhang Chen , Zhang Ping , Ibrahim Shehu Adam , Sun Zhipeng , Li Yuanming , Tang Chuanbao , Peng Qing , Lu Chenyang
Published: July 2024

Interstitial diffusion is important for radiation defect evolution in zirconium alloys. This study employed molecular dynamics simulations to investigate interstitial diffusion in α-Zr and its alloys with 1.0 at.% Nb and 1.0 at.% Sn using a variety of interatomic potentials. Pronounced differences in diffusion anisotropy were observed in pure Zr among the employed potentials. This was attributed to the considerable differences in migration barriers among the various interstitial configurations. The introduction of small concentrations of Nb and Sn solute atoms was found to significantly influence diffusion anisotropy by either directly participating in the diffusion process or altering the chemical environment around the diffusing species. Based on the moderate agreement of interstitial energetics in pure Zr, accurately describing interstitial diffusion in Zr alloys is expected to be more complex. This work underscores the importance of the careful validation and selection of interatomic potentials and highlights the need to understand the effects of solute atoms on interstitial diffusion.
Fault Diagnosis in Induction Motors through Infrared Thermal Images Using Convolutional Neural Network Feature Extraction
Authors: Calderon-Uribe Uriel , Lizarraga-Morales Rocio A. , Guryev Igor V.
Published: July 2024

The development of diagnostic systems for rotating machines such as induction motors (IMs) is a task of utmost importance for the industrial sector. Reliable diagnostic systems allow for the accurate detection of different faults. Different methods based on the acquisition of thermal images (TIs) have emerged as diagnosis systems for the detection of IM faults to prevent the further generation of faults. However, these methods are based on artisanal feature selection, so obtaining high accuracy rates is usually challenging. For this reason, in this work, a new system for fault detection in IMs based on convolutional neural networks (CNNs) and thermal images (TIs) is presented. The system is based on the training of a CNN using TIs to select and extract the most salient features of each fault present in the IM. Subsequently, a classifier based on a decision tree (DT) algorithm is trained using the features learned by the CNN to infer the motor conditions. The results of this methodology show an improvement in the accuracy, precision, recall, and F1-score metrics for 11 different conditions.
Brown Algae Ecklonia cava Extract Modulates Adipogenesis and Browning in 3T3-L1 Preadipocytes through HO-1/Nrf2 Signaling
Authors: Suryaningtyas Indyaswan T. , Lee Dae-Sung , Je Jae-Young
Published: July 2024

This study explores the anti-obesity effects of the ethyl acetate extract of Ecklonia cava (EC-ETAC) on 3T3-L1 preadipocytes, focusing on its impact on adipogenesis, lipolysis, and adipose browning via the HO-1/Nrf2 pathway. Western blot analysis revealed that EC-ETAC significantly inhibited adipogenic transcription factors (PPARγ, C/EBPα, SREBP-1) and lipogenesis-related proteins (FAS, LPL). Concurrently, EC-ETAC enhanced lipolytic markers (p-AMPK, p-HSL) and adipose browning-related proteins (UCP-1, PGC-1α), indicating its role in promoting lipolysis and adipose browning. The inhibition of HO-1 by zinc protoporphyrin (ZnPP) significantly reversed these effects, underscoring the critical role of HO-1 in mediating the anti-obesity properties of EC-ETAC. Additionally, fluorescence measurements and Oil Red O staining confirmed the reduction of lipid accumulation and oxidative stress upon EC-ETAC treatment. These findings suggest that EC-ETAC exerts its anti-obesity effects by modulating the HO-1/Nrf2 pathway, which is crucial for regulating adipogenesis, lipolysis, and adipose browning. This study highlights the potential of EC-ETAC as a natural therapeutic agent for obesity management and supports further research into its clinical applications. By targeting the HO-1/Nrf2 pathway, EC-ETAC could offer a novel approach to enhancing energy expenditure and reducing fat mass, thereby improving metabolic health.
Cardiometabolic Risk Factors Related to Atrial Fibrillation and Metabolic Syndrome in the Pakistani Population
Authors: Rafaqat Saira , Sharif Saima , Naz Shagufta , Gluscevic Sanja , Mercantepe Filiz , Ninic Ana , Klisic Aleksandra
Published: July 2024

Background and Objectives: This study aimed to examine the relationship between cardiometabolic risk factors and atrial fibrillation (AF) and the simultaneous presence of AF and metabolic syndrome (MetS) in the Pakistani population. Materials and Methods: A total of 690 subjects were enrolled (n = 230 patients with AF, n = 230 patients with AF and MetS, and n = 230 controls). The associations between cardiometabolic parameters and AF with and without MetS were analyzed by univariable and multivariable binary regression analyses. Results: Body mass index (BMI), fasting blood glucose (FBG), and triglycerides (TG) were independently positively correlated, but the glomerular filtration rate (GFR) and sodium were independently negatively correlated with AF. An increase in BMI, FBG, and TG levels by one unit measure increased the probability by 55.1%, 20.6%, and 1.3%, respectively, for the AF occurrence. A decrease in GFR and sodium levels increased the probability by 4.3% and 33.6%, respectively, for the AF occurrence. On the other hand, uric acid was independently negatively correlated, whereas sodium was independently positively correlated, with MetS and AF. A decrease in uric acid levels and an increase in sodium levels by 1 unit measure increased the probability for MetS and AF by 23.2% and 7.5%, respectively. Conclusions: Cost-effective and routinely measured parameters, i.e., BMI, FBG TG, GFR, and sodium levels, can be reliable indicators of AF, whereas serum uric acid and sodium levels are independently associated with AF and MetS in the Pakistani population. Timely recognition and the control of modifiable cardiometabolic risk factors are of great significance in the prevention of AF development.
Effect of Calefaction and Stress Relaxation on Grain Boundaries/Textures of Cu–Cr–Ni Alloy
Authors: Liu Haitao , Wang Guojie , Song Kexing , Hua Yunxiao , Liu Yong , Huang Tao
Published: July 2024

The Cu–Cr–Ni alloy is a key material for the manufacturing of connectors, which requires excellent resistance to stress relaxation. However, the inherent correlation among microstructure, texture, and properties is still unclear. In this study, we investigated the influence of calefaction and stress relaxation on the grain boundaries (GBs), textures, and properties of the Cu–Cr–Ni alloy. The results showed that calefaction and stress relaxation had opposite effects on GBs and textures. Calefaction led to a decrease in the proportion of low-angle grain boundaries (LAGBs), an increase in the Schmidt factor (SF) value of the grains, and a transition of texture from <111> to <113>. The grains with higher SF values were more susceptible to plastic deformation, which deteriorated the stress relaxation resistance. By comparison, stress relaxation led to an increase in the proportion of LAGBs, a decrease in SF values of the grains, and a transition of texture from <113> to <111> and <001>. After stress relaxation, the variation trends of the GBs and textures were consistent with those of other plastic deformations, indicating that stress relaxation can be verified by the variations in GBs and textures. Our findings provide a theoretical basis for improvements in stress relaxation resistance of the Cu-based alloys used in connector industry.
Osteonecrosis of the Jaws Associated with Herpes Zoster Infection: A Systematic Review and a Rare Case Report
Authors: Mancini Antonio , Chirico Fabrizio , Inchingolo Angelo Michele , Piras Fabio , Colonna Valeria , Marotti Pierluigi , Carone Claudio , Inchingolo Alessio Danilo , Inchingolo Francesco , Dipalma Gianna
Published: July 2024

The investigation’s goal was to obtain further knowledge about the connection between Herpes Zoster infection and dentistry therapy for the osteonecrosis of the jaws, combining the review with a case report relevant to the purpose. It is important to study this association because it is a possible additional factor to be considered in the causes of the osteonecrosis of the jaws. We limited our search to English-language papers published between 1 January 2004 and 7 June 2024 in PubMed, Scopus, and Web of Science that were relevant to our topic. In the search approach, the Boolean keywords “Herpes Zoster AND osteonecros*” were used. Results: This study analyzed 148 papers from Web of Science, PubMed, and Scopus, resulting in 95 articles after removing duplicates. Of these, 49 were removed because they were off topic, and 46 were confirmed. This study includes a qualitative analysis of the final 12 articles, removing 34 articles that were off topic. The literature highlights severe oral complications from Herpes Zoster reactivation, emphasizing the need for early diagnosis, comprehensive management, and multidisciplinary care. Treatment strategies include antiviral therapy, pain management, surgical debridement, and antibiotics. Immunocompromised individuals require vigilant monitoring and balanced immunosuppressive therapy. Further research is needed to enhance therapeutic approaches.
Potential of Sorghum Seeds in Alleviating Hyperglycemia, Oxidative Stress, and Glycation Damage
Authors: Ben El Mahdi Nora , Lemée Laurent , Remaury Quentin Blancart , Eloy Lilian , Nhiri Naima , Lakhssassi Naoufal , Cacciola Francesco , Nhiri Mohamed
Published: July 2024

Diabetes mellitus, characterized by dysregulated glucose metabolism, oxidative stress, and the formation of advanced glycation end products, poses a significant global health burden. In this study, we explored the potential of sorghum (Sorghum bicolor) seeds, known for their abundant phytochemical composition, as a natural remedy for diabetes and its associated damage. High-performance liquid chromatography/high-resolution mass spectrometry analysis revealed a remarkable phenolic richness in sorghum grains, including gallic acid, quercetin, and the predominant procyanidin B-1, with ecotype-specific variations in flavonoid distribution. Elemental analysis by ICP showed an abundance of macro-elements (Ca, K, Mg), trace elements (Fe, Mn, Si, Zn), and ultra-trace elements (B, Co, Cr, Cu, Mo, Se, V) essential for human health, supporting its therapeutic and nutritional potential. Additionally, the results demonstrated variable total phenolic contents (188–297 mg GAE/g dE) and total flavonoid contents (66–78 mg QE/g dE), with corresponding differences in antioxidant activities across the five ecotypes. Treatment with sorghum seed extract (SE1) significantly reduced oxidative stress markers, such as malondialdehyde (MDA)by 40% and hydrogen peroxide (H2O2) by 63%, in diabetic mice, compared to untreated diabetic controls. Moreover, sorghum extracts exhibited a remarkable increase in antioxidant enzyme activities, including a 50% increase in superoxide dismutase (SOD) activity and a 60% increase in glutathione peroxidase (GPx) activity, indicating their potential to bolster antioxidant defenses against diabetes-induced oxidative stress. These findings underscore the therapeutic potential of sorghum seeds in diabetes management and prevention, paving the way for the development of functional foods with enhanced health benefits.
Polarized-Speckle Deviation Imaging through Scattering Media under Strong Background Light Interference
Authors: He Si , Wang Xia , Li Linhao
Published: July 2024

A crucial challenge faced by noninvasive imaging through strongly scattering media is overcoming background light interference. Polarization-based anti-scattering methods can eliminate background light interference, but fail to utilize speckle images that do not contain unscattered object light for object reconstruction. Although speckle correlation imaging (SCI) methods can utilize speckle images for object reconstruction, it is difficult to achieve stable high-quality reconstruction and overcome background light interference using these methods. In this study, we propose a polarized-speckle deviation imaging (PSDI) method to overcome background light interference and achieve high-quality imaging through strongly scattering media. PSDI utilizes the bispectrum and autocorrelation of polarized speckle image deviations to reconstruct the Fourier phase and amplitude spectra of the object image, respectively. Experimental results show that when the background light is polarized and unpolarized, PSDI can achieve stable high-fidelity reconstruction of a polarized object when the signal-to-background ratio (SBR) is lower than −7 dB and −9 dB, respectively. PSDI bridges the gap between imaging with strongly scattered light and overcoming strong background light interference, and is expected to find widespread applications in fields such as biomedical imaging, astronomical observation, underwater imaging, and remote sensing.
An Improved and Simplified Agrobacterium-Mediated Genetic Transformation Protocol for Solanum nigrum with a Shorter Growth Time
Authors: Li Qianqian , Wang Xiuyuan , Teng Chong , He Xuxia , Fu Xinyue , Peng Wentao , Fan Yinglun , Lyu Shanhua
Published: July 2024

Solanum nigrum (Solanaceae family) is widely consumed as a fruit or local leafy vegetable after boiling; it also serves as a medicinal plant. Although Agrobacterium-mediated genetic transformation has been established in S. nigrum, the transformation period is long. Specifically, induction of roots takes approximately five weeks for tetraploid and hexaploid S. nigrum, and 7 weeks for diploid Solanum americanum. In this study, we developed an improved rooting-induced method that requires only about 1 week and avoids the use of tissue culture. After generating the transgenic shoots, they were directly transplanted into the soil to facilitate root formation. Remarkably, 100% of the transgenic shoots developed roots within 6 days. Our improved method is time-saving (saving more than 1 month) and simpler to operate. The improved rooting-induced step can be applied to induce roots in various plants using tissue culture, exemplified by the carnation (Dianthus caryophyllus L.). Furthermore, we applied the improved method to generate S. americanum plants expressing AcMYB110 from kiwifruit (Actinidia chinensis spp.). This method will contribute to speeding up gene functional analysis and trait improvement in S. nigrum and might have potential in fast plant molecular breeding processes in crops and rapid rooting induction in tissue culture.
Multilayer Film Comprising Polybutylene Adipate Terephthalate and Cellulose Nanocrystals with High Barrier and Compostable Properties
Authors: Melendez-Rodriguez Beatriz , Prieto Cristina , Pardo-Figuerez Maria , Angulo Inmaculada , Bourbon Ana I. , Amado Isabel R. , Cerqueira Miguel A. , Pastrana Lorenzo M. , Hilliou Loic Hugues Gilles , Vicente António A. , Cabedo Luis , Lagaron Jose M.
Published: July 2024

In the present study, a multilayer, high-barrier, thin blown film based on a polybutylene adipate terephthalate (PBAT) blend with polyhydroxyalkanoate (PHA), and composed of four layers including a cellulose nanocrystal (CNC) barrier layer and an electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) hot-tack layer, was characterized in terms of the surface roughness, surface tension, migration, mechanical and peel performance, barrier properties, and disintegration rate. The results showed that the film exhibited a smooth surface. The overall migration tests showed that the material is suitable to be used as a food contact layer. The addition of the CNC interlayer had a significant effect on the mechanical properties of the system, drastically reducing the elongation at break and, thus, the flexibility of the material. The film containing CNCs and electrospun PHBV hot-tack interlayers exhibited firm but not strong adhesion. However, the multilayer was a good barrier to water vapor (2.4 ± 0.1 × 10−12 kg·m−2·s−1·Pa−1), and especially to oxygen (0.5 ± 0.3 × 10−15 m3·m−2·s−1·Pa−1), the permeance of which was reduced by up to 90% when the CNC layer was added. The multilayer system disintegrated completely in 60 days. All in all, the multilayer system developed resulted in a fully compostable structure with significant potential for use in high-barrier food packaging applications.
Old Age in the Perspective of Faith: Elderly People in the Life of the Church
Authors: Brzeziński Mirosław
Published: July 2024

Elderly people make up an increasingly large part of society and the Church. The theme of old age is also increasingly present in papal teaching. This is evidenced, for example, by the series of Wednesday Catecheses on old age delivered by Pope Francis in 2022, or by the establishment of the World Day for Grandparents and the Elderly. Given the importance of the presence of the elderly, this article aims to present old age in the perspective of faith, and the importance of the presence of the elderly in the life of the Church. The author will therefore focus, first and foremost, on the view of old age as a time of thanksgiving and gratitude to God for the gift of life, for the goods received, the experiences lived, and the people encountered. Viewed from the perspective of faith, old age is also a time of preparation for death and for meeting the Risen Christ. It is therefore a time to adopt a new outlook on faith and the call to holiness. Old age is, at the same time, a period of life in which individuals can still contribute a lot to the life of the family, society, and the Church through their commitment, service, bearing witness to the faith, bearing witness to the Gospel and the values that flow from living the Gospel, through works of charity and, above all, through sharing the awareness that life is a beautiful gift from God that is worth using well and wisely. And, through the wisdom gained over the many years of their lives, elderly people become authentic witnesses of God’s love. The experience of long life and gratitude for this gift is not only relevant to Catholics and Christians, but has a universal appeal, since in any society, regardless of faith, human life should also be valued in old age.
The First Apocalypse of James in a Socio-Linguistic Perspective: Three Greek and Coptic Versions from Ancient Monastic Egypt
Authors: Kim David W.
Published: July 2024

The discovery of the Nag Hammadi Codices (NHC) in 1945 rates as one of the two most profound occurrences for Biblical archaeology and interpretation during the last hundred years, along with the Dead Sea Scrolls (1946–1956). The codices allow us to document Christian monastic culture, gnostic Christianity and gnostic offshoots in the desert climate of Late Ancient Egypt. The recovery of the related Codex Tchacos (CT) brought further excitement for contemporary readers by 2006, it being sensational that narratives of “Judas the betrayer” and “doubting Thomas” were found in the whole collection of writings. The text named the [First] Apocalypse of James, significantly, was found to be in both NHC and CT in different Coptic versions (from near the sacred sites of Chenoboskion and El Minya), but yet another more fragmentary version in Greek had turned up much earlier among the huge cache of papyri found at Oxyrhynchus (also, like the other places, on the banks of the Nile). Given the opportunity for comparison, what distinguishes the three versions? Does comparative analysis better tell us what this ancient text is about? Does the strong presence of Gnostic Christian insights in the Coptic texts still imply a historical Jamesian community is being honoured? This paper concentrates on three comparable passages in the three versions that apparently contain historical memories of James and his followers. It works on the reasonable hypothesis that the Greek version of Oxyrhynchus Papyri (P.Oxy. 5533) (hereafter = PO) is prior and read with different purposes than the two Coptic translated versions of CT (CT 2.10–30) and NHC (NHC V,3. 24–44). When a critical approach, involving a socio-linguistic comparison, is applied, we will see that the three versions of the text were not directly related to each other, but that narratives about James the Just were available to desert monastics from the second century CE. The paper argues for a literal transmission of traditions from a Jewish Christian community around James into Egypt, that the textual figure of James in the Oxyrhynchus fragments points to a ‘mutual familiarity’ between PO and CT, while the NHC tradition of James has been further elaborated by processes of compilation and addition.
Religious Transhumanism as a New Religious Movement: Sketching a Model of the Development of Religious Transhumanism
Authors: Lemasters Ryan
Published: July 2024

This essay proposes a new model for understanding religious transhumanism by extending existing frameworks that have been useful for predicting the success of new religious movements (NRMs). This paper focuses on the Mormon Transhumanist Association as a case of religious transhumanism that is incongruent with existing models of NRMs, thereby highlighting the limitations of these models. First, I demonstrate how the Morman Transhumanist Association challenges religious scholars’ conventional concepts for understanding NRMs, particularly within anthropology, cosmology, and eschatology. Then, I present a model that effectively accounts for the unique characteristics of religious transhumanist groups, thereby demonstrating and addressing the field’s current lack of an explanatory framework.
Spray-Dried Jaboticaba Powder as Food Resource
Authors: Silva Neiton C. , Andrade Gabriela B. , Barrozo Marcos A. S.
Published: July 2024

Jaboticaba, a popular Brazilian fruit, has recently garnered scientific interest due to its nutritional properties and high levels of bioactive compounds. However, this fruit is highly perishable due to its high moisture content and physical–chemical structure. Therefore, it is imperative to employ methods for preserving it and explore its potential as a food resource. This study investigates the use of a spray drying method to dehydrate whole jaboticaba fruits. The effects of air temperature (T) ranging from 67.9 to 132.1 °C, air flow rate (AF) from 1.54 to 1.86 m3/min, maltodextrin concentration (M) from 8.9 to 41.1%, and feed flow rate (FF) from 0.36 to 0.84 L/h on the moisture content, drying yield, and bioactive compounds (total phenolic, total flavonoid, citric acid, and ascorbic acid contents) were quantified. The results indicate that spray drying can produce a powder with reduced moisture content levels, a satisfactory drying yield, and high levels of bioactive compounds if performed under specific conditions. An optimization study using desirability analysis shows that having T, AF, M, and FF at 132.1 °C, 1.86 m3/min, 15.0%, and 0.4549 L/h, respectively, is the optimal condition for the studied variables. Spray drying has proven to be a very promising alternative for jaboticaba processing, enabling better applications as a food resource.