bluuebunny/crossref_metadata_2025 huggingface dataset:
Disc Thickness and Spacing Distance Impacts on Flow Characteristics of Multichannel Tesla Turbines
Authors: Qi Wenjiao , Deng Qinghua , Jiang Yu , Yuan Qi , Feng Zhenping
Published: December 2018

Tesla turbines are a kind of unconventional bladeless turbines, which utilize the viscosity of working fluid to rotate the rotor and realize energy conversion. They offer an attractive substitution for small and micro conventional bladed turbines due to two major advantages. In this study, the effects of two influential geometrical parameters, disc thickness and disc spacing distance, on the aerodynamic performance and flow characteristics for two kinds of multichannel Tesla turbines (one-to-one turbine and one-to-many turbine) were investigated and analyzed numerically. The results show that, with increasing disc thickness, the isentropic efficiency of the one-to-one turbine decreases a little and that of the one-to-many turbine reduces significantly. For example, for turbine cases with 0.5 mm disc spacing distance, the former drops less than 7% and the latter decreases by about 45% of their original values as disc thickness increases from 1 mm to 2 mm. With increasing disc spacing distance, the isentropic efficiency of both kinds of turbines increases first and then decreases, and an optimal value and a high efficiency range exist to make the isentropic efficiency reach its maximum and maintain at a high level, respectively. The optimal disc spacing distance for the one-to-one turbine is less than that for the one-to-many turbine (0.5 mm and 1 mm, respectively, for turbine cases with disc thickness of 1 mm). To sum up, for designing a multichannel Tesla turbine, the disc spacing distance should be among its high efficiency range, and the determination of disc thickness should be balanced between its impacts on the aerodynamic performance and mechanical stress.
The Relic Trochodendron aralioides Siebold & Zucc. (Trochodendraceae) in Taiwan: Ensemble Distribution Modeling and Climate Change Impacts
Authors: Lin Cheng-Tao , Chiu Ching-An
Published: December 2018

Trochodendron aralioides Siebold & Zuccarini (Trochodendraceae) is a famous relic tree species. Understanding the comprehensive spatial distribution and likely impacts of climate change on T. aralioides in its main habitat—Taiwan—is of great importance. We collected occurrence data and bioclimatic data to predict the current and future (year 2050) distribution by ensemble distribution modeling on the BIOMOD2 platform. Visualization of occurrence point data revealed that the main population of T. aralioides was concentrated at medium altitudes and extended to both ends of Taiwan, being especially rich in the northern low mountains. A similar distribution pattern of occurrence probability was shown by ensemble prediction of the true skill statistic >0.8 models. Comparing the current and future distribution of T. aralioides, the overlay analysis with profile display demonstrated spatial turnover that revealed a discrepancy between different latitudes and altitudes. In the future climate, T. aralioides at the middle altitudes of central Taiwan could migrate upward, but its population in northern Taiwan could lose most of its habitat. Consequently, T. aralioides in the low mountains of northern Taiwan could be particularly in need of further conservation research, which is urgently required to mitigate climate change impacts.
Potential of the Probiotic Lactobacillus Plantarum ATCC 14917 Strain to Produce Functional Fermented Pomegranate Juice
Authors: Mantzourani Ioanna , Kazakos Stavros , Terpou Antonia , Alexopoulos Athanasios , Bezirtzoglou Eugenia , Bekatorou Argyro , Plessas Stavros
Published: December 2018

In this research survey the application of probiotic strain Lactobacillus plantarum ATCC 14917 in pomegranate juice fermentation is sought. Pomegranate juice was fermented for 24 h and then it was stored 4 for 4 weeks. Cell viability retained in high levels after the 24 h of fermentation and storage for 4 weeks (above 8.8 log cfu/mL), while fermented pomegranate juice was scored better at the 4th week of storage compared to non-fermented pomegranate juice. The probiotic strain was effective regarding lactic acid fermentation as was proved through sugar and organic acids analysis. Concentration of ethanol was maintained at low levels (0.3–1% v/v). Fermented pomegranate juice contained more and in higher percentages desirable volatile compounds (alcohols, ketones and esters) even at the 4th week of cold storage compared to non-fermented juice. Antioxidant activity (150.63 mg Trolox equivalent (TE)/100 mL at the 2nd week) and total phenolic content (206.46 mg gallic acid equivalents (GAE)/100 mL at the 2nd week) were recorded in higher levels for all the storage time compared to non-fermented juice.
SMC5/6: Multifunctional Player in Replication
Authors: Palecek Jan J.
Published: December 2018

The genome replication process is challenged at many levels. Replication must proceed through different problematic sites and obstacles, some of which can pause or even reverse the replication fork (RF). In addition, replication of DNA within chromosomes must deal with their topological constraints and spatial organization. One of the most important factors organizing DNA into higher-order structures are Structural Maintenance of Chromosome (SMC) complexes. In prokaryotes, SMC complexes ensure proper chromosomal partitioning during replication. In eukaryotes, cohesin and SMC5/6 complexes assist in replication. Interestingly, the SMC5/6 complexes seem to be involved in replication in many ways. They stabilize stalled RFs, restrain RF regression, participate in the restart of collapsed RFs, and buffer topological constraints during RF progression. In this (mini) review, I present an overview of these replication-related functions of SMC5/6.
Reference Genomes from Distantly Related Species Can Be Used for Discovery of Single Nucleotide Polymorphisms to Inform Conservation Management
Authors: Galla Stephanie J. , Forsdick Natalie J. , Brown Liz , Hoeppner Marc P. , Knapp Michael , Maloney Richard F. , Moraga Roger , Santure Anna W. , Steeves Tammy E.
Published: December 2018

Threatened species recovery programmes benefit from incorporating genomic data into conservation management strategies to enhance species recovery. However, a lack of readily available genomic resources, including conspecific reference genomes, often limits the inclusion of genomic data. Here, we investigate the utility of closely related high-quality reference genomes for single nucleotide polymorphism (SNP) discovery using the critically endangered kakī/black stilt (Himantopus novaezelandiae) and four Charadriiform reference genomes as proof of concept. We compare diversity estimates (i.e., nucleotide diversity, individual heterozygosity, and relatedness) based on kakī SNPs discovered from genotyping-by-sequencing and whole genome resequencing reads mapped to conordinal (killdeer, Charadrius vociferus), confamilial (pied avocet, Recurvirostra avosetta), congeneric (pied stilt, Himantopus himantopus) and conspecific reference genomes. Results indicate that diversity estimates calculated from SNPs discovered using closely related reference genomes correlate significantly with estimates calculated from SNPs discovered using a conspecific genome. Congeneric and confamilial references provide higher correlations and more similar measures of nucleotide diversity, individual heterozygosity, and relatedness. While conspecific genomes may be necessary to address other questions in conservation, SNP discovery using high-quality reference genomes of closely related species is a cost-effective approach for estimating diversity measures in threatened species.
Mechanisms of DNA Damage Tolerance: Post-Translational Regulation of PCNA
Authors: Leung Wendy , Baxley Ryan M. , Moldovan George-Lucian , Bielinsky Anja-Katrin
Published: December 2018

DNA damage is a constant source of stress challenging genomic integrity. To ensure faithful duplication of our genomes, mechanisms have evolved to deal with damage encountered during replication. One such mechanism is referred to as DNA damage tolerance (DDT). DDT allows for replication to continue in the presence of a DNA lesion by promoting damage bypass. Two major DDT pathways exist: error-prone translesion synthesis (TLS) and error-free template switching (TS). TLS recruits low-fidelity DNA polymerases to directly replicate across the damaged template, whereas TS uses the nascent sister chromatid as a template for bypass. Both pathways must be tightly controlled to prevent the accumulation of mutations that can occur from the dysregulation of DDT proteins. A key regulator of error-prone versus error-free DDT is the replication clamp, proliferating cell nuclear antigen (PCNA). Post-translational modifications (PTMs) of PCNA, mainly by ubiquitin and SUMO (small ubiquitin-like modifier), play a critical role in DDT. In this review, we will discuss the different types of PTMs of PCNA and how they regulate DDT in response to replication stress. We will also cover the roles of PCNA PTMs in lagging strand synthesis, meiotic recombination, as well as somatic hypermutation and class switch recombination.
Heavy Metal Resistance Determinants of the Foodborne Pathogen Listeria monocytogenes
Authors: Parsons Cameron , Lee Sangmi , Kathariou Sophia
Published: December 2018

Listeria monocytogenes is ubiquitous in the environment and causes the disease listeriosis. Metal homeostasis is one of the key processes utilized by L. monocytogenes in its role as either a saprophyte or pathogen. In the environment, as well as within an animal host, L. monocytogenes needs to both acquire essential metals and mitigate toxic levels of metals. While the mechanisms associated with acquisition and detoxification of essential metals such as copper, iron, and zinc have been extensively studied and recently reviewed, a review of the mechanisms associated with non-essential heavy metals such as arsenic and cadmium is lacking. Resistance to both cadmium and arsenic is frequently encountered in L. monocytogenes, including isolates from human listeriosis. In addition, a growing body of work indicates the association of these determinants with other cellular functions such as virulence, suggesting the importance of further study in this area.
Lateral Momentum Fluxes at the Confluence of the Negro and Solimões Rivers
Authors: Coutinho de Lima Adriano
Published: December 2018

Hydrodynamic zones of river confluences are remarkable not only for the turbulent mixing induced by the shear layer at the center of the mixing interface but also for the lateral momentum fluxes associated with channel topography. Detailed characterizations of lateral momentum transfers in river confluences, however, are few. In this study, contributions to the lateral momentum fluxes in the confluence of the Negro and Solimões rivers in Brazil were calculated based on a comprehensive set of field data. Results show that the lateral fluxes by the mean flow exceed the turbulent fluxes by two orders of magnitude. Furthermore, the Reynolds stress along the far field of the Solimões side of the Amazon channel scales with or surpasses the Reynolds stress at the interface with the Negro side. The importance of the shear layer in the lateral mixing is thus overshadowed by the competing hydrodynamic processes. This configuration partially explains the long distance required to complete the mixing of the waters of the two tributary rivers.
Comparing Inpatient Complication Rates between Octogenarians and Nonagenarians following Primary and Revision Total Knee Arthroplasty in a Nationally Representative Sample, 2010–2014
Authors: Smith Eric L , Dugdale Evan M , Tybor David , Kain Michael
Published: December 2018

We compared the inpatient postoperative complication rates between octogenarians and nonagenarians undergoing primary and revision total knee arthroplasty (TKA). We used the Nationwide Inpatient Sample (NIS) to analyze inpatient admission data from 2010–2014. We compared the rates at which nonagenarians and octogenarians developed each complication following both primary TKA (PTKA) and revision TKA (RTKA). A national estimate of 324,933 patients were included in our study. A total of 313,299 (96.42%) were octogenarians, and 11,634 (3.58%) were nonagenarians. 294,462 (90.62%) underwent PTKA, and 30,471 (9.38%) underwent RTKA. Nonagenarians undergoing PTKA had a higher inpatient mortality rate, and developed sepsis more frequently than octogenarians. Nonagenarians undergoing RTKA had a higher inpatient mortality rate, and developed cardiogenic shock more frequently than octogenarians. In both PTKA and RTKA, nonagenarians received transfusions more frequently, and developed urinary tract infection and acute kidney injury more frequently than octogenarians. In both PTKA and RTKA, nonagenarians sustained a higher inpatient mortality rate than octogenarians. Orthopedic surgeons should counsel nonagenarian patients undergoing both PTKA and RTKA preoperatively about this increased mortality risk, as well as the increased risks of more minor complications like transfusion, urinary tract infection, and acute kidney injury.
Estimating the Sediment Flux and Budget for a Data Limited Rift Valley Lake in Ethiopia
Authors: Aga Alemu O. , Melesse Assefa M. , Chane Bayou
Published: December 2018

Information on sediment concentration in rivers is important for the design and management of reservoirs. In this paper, river sediment flux and siltation rate of a rift valley lake basin (Lake Ziway, Ethiopia) was modeled using suspended sediment concentration (SSC) samples from four rivers and lake outlet stations. Both linear and non-linear least squares log–log regression methods were used to develop the model. The best-fit model was tested and evaluated qualitatively by time-series plots, quantitatively by using watershed model evaluation statistics, and validated by calculating the prediction error. Sediment yield (SY) of ungauged rivers were assessed by developing and using a model that includes catchment area, slope, and rainfall, whereas bedload was estimated. As a result, the gross annual SY transported into the lake was 2.081 Mton/year. Annually, 0.178 Mton/year of sediment is deposited in floodplains with a sediment trapping rate of 20.6%, and 41,340 ton/year of sediment leaves the lake through the Bulbula River. The annual sediment deposition in the lake is 2.039 Mton/year with a mean sediment trapping efficiency of 98%. Based on the established sediment budget with average rainfall, the lake will lose its volume by 0.106% annually and the lifetime of Lake Ziway will be 947 years. The results show that the approach used can be replicated at other similar ungauged watersheds. As one of the most important sources of water for irrigation in the country, the results can be used for planning and implementing a lake basin management program targeting upstream soil erosion control.
Prenatal Household Air Pollution Alters Cord Blood Mononuclear Cell Mitochondrial DNA Copy Number: Sex-Specific Associations
Authors: Kaali Seyram , Jack Darby , Delimini Rupert , Hu Lisa , Burkart Katrin , Opoku-Mensah Jones , Quinn Ashlinn , Ae-Ngibise Kenneth , Wylie Blair , Boamah-Kaali Ellen , Chillrud Steven , Owusu-Agyei Seth , Kinney Patrick , Baccarelli Andrea , Asante Kwaku , Lee Alison Gladding
Published: December 2018

Background: Associations between prenatal household air pollution (HAP) exposure or cookstove intervention to reduce HAP and cord blood mononuclear cell (CBMC) mitochondrial deoxyribonucleic acid copy number (mtDNAcn), an oxidative stress biomarker, are unknown. Materials and Methods: Pregnant women were recruited and randomized to one of two cookstove interventions, including a clean-burning liquefied petroleum gas (LPG) stove, or control. Prenatal HAP exposure was determined by serial, personal carbon monoxide (CO) measurements. CBMC mtDNAcn was measured by quantitative polymerase chain reaction. Multivariable linear regression determined associations between prenatal CO and cookstove arm on mtDNAcn. Associations between mtDNAcn and birth outcomes and effect modification by infant sex were explored. Results: LPG users had the lowest CO exposures (p = 0.02 by ANOVA). In boys only, average prenatal CO was inversely associated with mtDNAcn (β = -14.84, SE = 6.41, p = 0.03, per 1ppm increase in CO). When examined by study arm, LPG cookstove had the opposite effect in all children (LPG β = 19.34, SE = 9.72, p = 0.049), but especially boys (β = 30.65, SE = 14.46, p = 0.04), as compared to Control. Increased mtDNAcn was associated with improved birth outcomes. Conclusions: Increased prenatal HAP exposure reduces CBMC mtDNAcn, suggesting cumulative prenatal oxidative stress injury. An LPG stove intervention may reverse this effect. Boys appear most susceptible.
Challenges and Lessons Learned from Multi-Level Multi-Component Interventions to Prevent and Reduce Childhood Obesity
Authors: Gittelsohn Joel , Novotny Rachel , Trude Angela Cristina Bizzotto , Butel Jean , Mikkelsen Bent Egberg
Published: December 2018

Multi-level multi-component (MLMC) strategies have been recommended to prevent and reduce childhood obesity, but results of such trials have been mixed. The present work discusses lessons learned from three recently completed MLMC interventions to inform future research and policy addressing childhood obesity. B’more Healthy Communities for Kids (BHCK), Children’s Healthy Living (CHL), and Health and Local Community (SoL) trials had distinct cultural contexts, global regions, and study designs, but intervened at multiple levels of the socioecological model with strategies that address multiple components of complex food and physical activity environments to prevent childhood obesity. We discuss four common themes: (i) How to engage with community partners and involve them in development of intervention and study design; (ii) build and maintain intervention intensity by creating mutual promotion and reinforcement of the intervention activities across the multiple levels and components; (iii) conduct process evaluation for monitoring, midcourse corrections, and to engage stakeholder groups; and (iv) sustaining MLMC interventions and its effect by developing enduring and systems focused collaborations. The paper expands on each of these themes with specific lessons learned and presents future directions for MLMC trials.
Is Patient-Provider Racial Concordance Associated with Hispanics’ Satisfaction with Health Care?
Authors: Oguz Tunay
Published: December 2018

This study adds a gender approach to determine how patient provider racial concordance and acculturation affect Hispanics’ satisfaction with care and inform more nuanced approaches to improving the quality of care for this population. Using the Medical Expenditure Panel Surveys (MEPS) from 2009–2011, four binary satisfaction outcome measures were created from the MEPS: “doctor showed respect”, “spent enough time”, “explained things in a way you could understand”, and “listened carefully”. Next, a Probit model was employed to estimate the impact of racial concordance and acculturation on the probability of being satisfied with provider care for both male and female Hispanics. For Hispanic women, no significant association was found for the relationship between patient-provider concordance and the overall satisfaction with their care. Hispanic men were found to be less likely to be satisfied with some aspects of their medical care when they were racially concordant with their provider. Overcoming assumptions about shared identity is a crucial step in providing culturally competent care for all patients. There is a need for additional considerations in medical training to help physicians connect with patients, regardless of any type of observable concordance.
Food Reference Budgets as a Potential Policy Tool to Address Food Insecurity: Lessons Learned from a Pilot Study in 26 European Countries
Authors: Carrillo-Álvarez Elena , Penne Tess , Boeckx Hilde , Storms Bérénice , Goedemé Tim
Published: December 2018

The aim of this article is to present the development of cross-country comparable food reference budgets in 26 European countries, and to discuss their usefulness as an addition to food-based dietary guidelines (FBDG) for tackling food insecurity in low-income groups. Reference budgets are illustrative priced baskets containing the minimum goods and services necessary for well-described types of families to have an adequate social participation. This study was conducted starting from national FBDG, which were translated into monthly food baskets. Next, these baskets were validated in terms of their acceptability and feasibility through focus group discussions, and finally they were priced. Along the paper, we show how that food reference budgets hold interesting contributions to the promotion of healthy eating and prevention of food insecurity in low-income contexts in at least four ways: (1) they show how a healthy diet can be achieved with limited economic resources, (2) they bring closer to the citizen a detailed example of how to put FBDG recommendations into practice, (3) they ensure that food security is achieved in an integral way, by comprising the biological but also psychological and social functions of food, and (4) providing routes for further (comparative) research into food insecurity.
Outdoor Air Emissions, Land Use, and Land Cover around Schools on Tribal Lands
Authors: Barros Nirmalla , Tulve Nicolle , Bailey Ken , Heggem Daniel
Published: December 2018

Children from tribes are more burdened with adverse respiratory well-being outcomes versus other U.S. children. The objectives of this study were to identify stressors from the built and natural environments for tribal school-aged children. Outdoor air concentrations around U.S. tribal schools were linked to National Emission Inventories; ecoregions and National Land Cover Database; and American Community Survey and school map layers. Nine school sites (seven tribes, five U.S. states) were in three ecoregions: North American Deserts, Northern Forests, and Mediterranean California. Closest emission sources were oil, gas, airport, and manufacturing facilities. Maximum annual outdoor air concentrations were measured for toluene at two schools (29 ppb and 15 ppb, 2011), located four miles from a solid waste landfill and eight miles from paperboard/saw mills. Maximum annual concentrations of metals in particulate matter 10 micrometers and smaller were highest for manganese (68 ng/m3, 2011). Schools were in mainly arid and heavily forested lands. Closest emission sources were predominantly off tribal lands. Measurements were limited (<30/year). Compared to schools off tribal lands, schools on tribal lands were further away from roadway sources. Future research may examine outdoor air quality around schools with more developed land and indoor air for tribal children’s total exposure.
OPENCRONIC Study. Knowledge and Experiences of Spanish Patients and Carers about Chronic Disease
Authors: Casariego Emilio , Cebrián-Cuenca Ana M. , Llisterri José Luis , Micó-Pérez Rafael Manuel , Orozco-Beltran Domingo , Otero-Cacabelos Mercedes , Román-Sánchez Pilar , Sáez Francisco José
Published: December 2018

Background: Chronic diseases are currently the main cause of morbidity and mortality and represent a major challenge to healthcare systems. The objective of this study is to know Spanish public opinion about chronic disease and how it affects their daily lives. Methods: Through a telephone or online survey of 24 questions, data was gathered on the characteristics of the respondents and their knowledge and experiences of chronic diseases. Results: Of the 2522 survey respondents, 325 had a chronic disease and were carers, 1088 had a chronic disease and were not carers, 140 did not have a chronic disease but were carers, and 969 did not have chronic disease and were not carers. The degree of knowledge on these diseases was good or very good for 69.4%, 56.0%, 62.2%, and 46.7%, respectively, for each group. All the groups agreed that chronic diseases mainly affect mood, quality of life and having to make sacrifices. Conclusions: Knowledge about chronic diseases is relatively good, although it can be improved among the Spanish population, especially among patients who report having a chronic disease and play the role of carers. However, it is important to continue maintaining the level of information and training concerning these diseases.
Mapping Long-Term Dynamics of Population and Dwellings Based on a Multi-Temporal Analysis of Urban Morphologies
Authors: Hecht Robert , Herold Hendrik , Behnisch Martin , Jehling Mathias
Published: December 2018

Information on the distribution and dynamics of dwellings and their inhabitants is essential to support decision-making in various fields such as energy provision, land use planning, risk assessment and disaster management. However, as various different of approaches to estimate the current distribution of population and dwellings exists, further evidence on past dynamics is needed for a better understanding of urban processes. This article therefore addresses the question of whether and how accurately historical distributions of dwellings and inhabitants can be reconstructed with commonly available geodata from national mapping and cadastral agencies. For this purpose, an approach for the automatic derivation of such information is presented. The data basis is constituted by a current digital landscape model and a 3D building model combined with historical land use information automatically extracted from historical topographic maps. For this purpose, methods of image processing, machine learning, change detection and dasymetric mapping are applied. The results for a study area in Germany show that it is possible to automatically derive decadal historical patterns of population and dwellings from 1950 to 2011 at the level of a 100 m grid with slight underestimations and acceptable standard deviations. By a differentiated analysis we were able to quantify the errors for different urban structure types.
A Novel Transcription Factor CaSBP12 Gene Negatively Regulates the Defense Response against Phytophthora capsici in Pepper (Capsicum annuum L.)
Authors: Zhang Huai-Xia , Ali Muhammad , Feng Xiao-Hui , Jin Jing-Hao , Huang Liu-Jun , Khan Abid , Lv Jing-Gang , Gao Su-Yan , Luo De-Xu , Gong Zhen-Hui
Published: December 2018

SBP-box (Squamosa-promoter binding protein) genes are a type of plant-specific transcription factor and play important roles in plant growth, signal transduction and stress response. However, little is known about the SBP-box genes in pepper (CaSBP), especially in the process of Phytophthora capsici infection. In this study, a novel gene (CaSBP12) was selected from the CaSBP gene family, which was isolated from the pepper genome database in our previous study. The CaSBP12 gene was located in the nucleus of the cell and its silencing in the pepper plant enhanced the defense response against Phytophthora capsici infection. After inoculation with Phytophthora capsici, the root activity of the CaSBP12-silenced plants is compared to control plants, while malondialdehyde (MDA) content is compared viceversa. Additionally, the expression of defense related genes (CaPO1, CaSAR8.2, CaBPR1, and CaDEF1) in the silenced plants were induced to different degrees and the peak of CaSAR8.2 and CaBPR1 were higher than that of CaDEF1. The CaSBP12 over-expressed Nicotiana benthamiana plants were more susceptible to Phytophthora capsici infection with higher EC (electrical conductivity) and MDA contents as compared to the wild-type. The relative expression of defense related genes (NbDEF, NbNPR1, NbPR1a, and NbPR1b) in transgenic and wild-type Nicotiana benthamiana plants were induced, especially the NbPR1a and NbPR1b. In conclusion, these results indicate that CaSBP12 gene negative regulates the defense response against Phytophthora capsici infection which suggests their potentially significant role in plant defense. To our knowledge, this is the first report on CaSBP gene which negative regulate defense response.
Biosynthesis of Natural Rubber: Current State and Perspectives
Authors: Men Xiao , Wang Fan , Chen Guo-Qiang , Zhang Hai-Bo , Xian Mo
Published: December 2018

Natural rubber is a kind of indispensable biopolymers with great use and strategic importance in human society. However, its production relies almost exclusively on rubber-producing plants Hevea brasiliensis, which have high requirements for growth conditions, and the mechanism of natural rubber biosynthesis remains largely unknown. In the past two decades, details of the rubber chain polymerization and proteins involved in natural rubber biosynthesis have been investigated intensively. Meanwhile, omics and other advanced biotechnologies bring new insight into rubber production and development of new rubber-producing plants. This review summarizes the achievements of the past two decades in understanding the biosynthesis of natural rubber, especially the massive information obtained from the omics analyses. Possibilities of natural rubber biosynthesis in vitro or in genetically engineered microorganisms are also discussed.
Role of HDAC3-miRNA-CAGE Network in Anti-Cancer Drug-Resistance
Authors: Kwon Yoojung , Kim Youngmi , Jung Hyun Suk , Jeoung Dooil
Published: December 2018

Histone modification is associated with resistance to anti-cancer drugs. Epigenetic modifications of histones can regulate resistance to anti-cancer drugs. It has been reported that histone deacetylase 3 (HDAC3) regulates responses to anti-cancer drugs, angiogenic potential, and tumorigenic potential of cancer cells in association with cancer-associated genes (CAGE), and in particular, a cancer/testis antigen gene. In this paper, we report the roles of microRNAs that regulate the expression of HDAC3 and CAGE involved in resistance to anti-cancer drugs and associated mechanisms. In this review, roles of HDAC3-miRNAs-CAGE molecular networks in resistance to anti-cancer drugs, and the relevance of HDAC3 as a target for developing anti-cancer drugs are discussed.
Protective Effect of Colla corii asini against Lung Injuries Induced by Intratracheal Instillation of Artificial Fine Particles in Rats
Authors: Liu Tiantian , Zhang Piaopiao , Ling Yahao , Hu Guang , Gu Jianjun , Yang Hong , Wei Jinfeng , Wang Aiping , Jin Hongtao
Published: December 2018

Environmental issues pose huge threats to public health, particularly the damage caused by fine particulate matter (PM2.5). However, the mechanisms of injury require further investigation and medical materials that can protect the lungs from PM2.5 are needed. We have found that Colla corii asini, a traditional Chinese medicine that has long been used to treat various ailments, is a good candidate to serve this purpose. To understand the mechanisms of PM2.5-induced lung toxicity and the protective effects of Colla corii asini, we established a rat model of lung injury via intratracheal instillation of artificial PM2.5 (aPM2.5). Our results demonstrated that Colla corii asini significantly protected against lung function decline and pathologic changes. Inflammation was ameliorated by suppression of Arg-1 to adjust the disturbed metabolic pathways induced by aPM2.5, such as arginine and nitrogen metabolism and aminoacyl-tRNA biosynthesis, for 11 weeks. Our work found that metabolomics was a useful tool that contributed to further understanding of PM2.5-induced respiratory system damage and provided useful information for further pharmacological research on Colla corii asini, which may be valuable for therapeutic intervention.
Innovative Strategy for 3D Transfection of Primary Human Stem Cells with BMP-2 Expressing Plasmid DNA: A Clinically Translatable Strategy for Ex Vivo Gene Therapy
Authors: Paidikondala Maruthibabu , Kadekar Sandeep , Varghese Oommen P.
Published: December 2018

Ex vivo gene therapy offers enormous potential for cell-based therapies, however, cumbersome in vitro cell culture conditions have limited its use in clinical practice. We have optimized an innovative strategy for the transient transfection of bone morphogenetic protein-2 (BMP-2) expressing plasmids in suspended human stem cells within 5-min that enables efficient loading of the transfected cells into a 3D hydrogel system. Such a short incubation time for lipid-based DNA nanoparticles (lipoplexes) reduces cytotoxicity and at the same time reduces the processing time for cells to be transplanted. The encapsulated human mesenchymal stromal/stem cells (hMSCs) transfected with BMP-2 plasmid demonstrated high expression of an osteogenic transcription factor, namely RUNX2, but not the chondrogenic factor (SOX9), within the first three days. This activation was also reflected in the 7-day and 21-day experiment, which clearly indicated the induction of osteogenesis but not chondrogenesis. We believe our transient transfection method demonstrated in primary MSCs can be adapted for other therapeutic genes for different cell-based therapeutic applications.
Periostin Mediates Right Ventricular Failure through Induction of Inducible Nitric Oxide Synthase Expression in Right Ventricular Fibroblasts from Monocrotaline-Induced Pulmonary Arterial Hypertensive Rats
Authors: Imoto Keisuke , Okada Muneyoshi , Yamawaki Hideyuki
Published: December 2018

Pulmonary arterial hypertension (PAH) leads to lethal right ventricular failure (RVF). Periostin (POSTN) mRNA expression is increased in right ventricles (RVs) of monocrotaline (MCT)-induced PAH model rats. However, the pathophysiological role of POSTN in RVF has not been clarified. We investigated the effects of POSTN on inducible nitric oxide (NO) synthase (iNOS) expression and NO production, which causes cardiac dysfunction, in right ventricular fibroblasts (RVFbs). Male Wistar rats were intraperitoneally injected with MCT (60 mg/kg) or saline. Three weeks after injection, RVFbs were isolated from RVs of MCT- or saline-injected rats (MCT-RVFb or CONT-RVFb). In MCT-RVFb, iNOS expression and phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun N-terminal kinase (JNK) and nuclear factor-kappa B (NF-κB) were higher than those in CONT-RVFb. Recombinant POSTN increased iNOS expression and NO production, which were prevented by a pharmacological inhibition of ERK1/2, JNK or NF-κB in RVFbs isolated from normal rats. Culture medium of POSTN-stimulated RVFbs suppressed Ca2+ inflow through l-type Ca2+ channel (LTCC) in H9c2 cardiomyoblasts. We demonstrated that POSTN enhances iNOS expression and subsequent NO production via ERK1/2, JNK, and NF-κB signaling pathways in RVFbs. POSTN might mediate RVF through the suppression of LTCC activity of cardiomyocytes by producing NO from RVFbs in PAH model rats.
TRIMming down to TRIM37: Relevance to Inflammation, Cardiovascular Disorders, and Cancer in MULIBREY Nanism
Authors: Brigant Benjamin , Metzinger-Le Meuth Valérie , Rochette Jacques , Metzinger Laurent
Published: December 2018

TRIpartite motif (TRIM) proteins are part of the largest subfamilies of E3 ligases that mediate the transfer of ubiquitin to substrate target proteins. In this review, we focus on TRIM37 in the normal cell and in pathological conditions, with an emphasis on the MULIBREY (MUscle-LIver-BRain-EYe) genetic disorder caused by TRIM37 mutations. TRIM37 is characterized by the presence of a RING domain, B-box motifs, and a coiled-coil region, and its C-terminal part includes the MATH domain specific to TRIM37. MULIBREY nanism is a rare autosomal recessive caused by TRIM37 mutations and characterized by severe pre- and postnatal growth failure. Constrictive pericarditis is the most serious anomaly of the disease and is present in about 20% of patients. The patients have a deregulation of glucose and lipid metabolism, including type 2 diabetes, fatty liver, and hypertension. Puzzlingly, MULIBREY patients, deficient for TRIM37, are plagued with numerous tumors. Among non-MULIBREY patients affected by cancer, a wide variety of cancers are associated with an overexpression of TRIM37. This suggests that normal cells need an optimal equilibrium in TRIM37 expression. Finding a way to keep that balance could lead to potential innovative drugs for MULIBREY nanism, including heart condition and carcinogenesis treatment.
Thermococcus Eurythermalis Endonuclease IV Can Cleave Various Apurinic/Apyrimidinic Site Analogues in ssDNA and dsDNA
Authors: Wang Wei-Wei , Zhou Huan , Xie Juan-Juan , Yi Gang-Shun , He Jian-Hua , Wang Feng-Ping , Xiao Xiang , Liu Xi-Peng
Published: December 2018

Endonuclease IV (EndoIV) is a DNA damage-specific endonuclease that mainly hydrolyzes the phosphodiester bond located at 5′ of an apurinic/apyrimidinic (AP) site in DNA. EndoIV also possesses 3′-exonuclease activity for removing 3′-blocking groups and normal nucleotides. Here, we report that Thermococcus eurythermalis EndoIV (TeuendoIV) shows AP endonuclease and 3′-exonuclease activities. The effect of AP site structures, positions and clustered patterns on the activity was characterized. The AP endonuclease activity of TeuendoIV can incise DNA 5′ to various AP site analogues, including the alkane chain Spacer and polyethylene glycol Spacer. However, the short Spacer C2 strongly inhibits the AP endonuclease activity. The kinetic parameters also support its preference to various AP site analogues. In addition, the efficient cleavage at AP sites requires ≥2 normal nucleotides existing at the 5′-terminus. The 3′-exonuclease activity of TeuendoIV can remove one or more consecutive AP sites at the 3′-terminus. Mutations on the residues for substrate recognition show that binding AP site-containing or complementary strand plays a key role for the hydrolysis of phosphodiester bonds. Our results provide a comprehensive biochemical characterization of the cleavage/removal of AP site analogues and some insight for repairing AP sites in hyperthermophile cells.
Research Advances and Prospects on Mechanism of Sinomenin on Histamine Release and the Binding to Histamine Receptors
Authors: Zhang Yu-Shi , Han Jia-Yin , Iqbal Omer , Liang Ai-Hua
Published: December 2018

Sinomenine (SIN) is widely used in China to treat a variety of rheumatic diseases (RA), and has various pharmacological effects such as anti-inflammatory, analgesic, and anti-tumor effects. However, due to the histamine release characteristics of SIN, its adverse reactions such as allergic reactions, gastrointestinal reactions, and circulatory systemic reactions have been drawing increasing attention. We present here a systematic review of the chemical structure, pharmacological effects, clinical application, and adverse reactions of SIN, a detailed discussion on the relationship between histamine/histamine receptor and mechanism of action of SIN. In addition, we simulated the binding of SIN to four histamine receptors by using a virtual molecular docking method and found that the bonding intensity between SIN and receptors varied in the order shown as follows: H1R > H2R ~ H3R > H4R. The docking results suggested that SIN might exhibit dual regulatory effects in many processes such as cyclooxygenase-2 (COX-2) expression, NF-κB pathway activation, and degranulation of mast cells to release histamine, thereby exhibiting pro-inflammatory (adverse reactions)/anti-inflammatory effects. This study provides a theoretical basis for the clinical treatment of inflammations seen such as in RA using SIN, and also suggests that SIN has great potential in the field of cancer treatment and will have very important social and economic significance.
Evaluation of a Rehabilitation System for the Elderly in a Day Care Center
Authors: Palestra Giuseppe , Rebiai Mohamed , Courtial Estelle , Koutsouris Dimitrios
Published: December 2018

This paper presents a rehabilitation system based on a customizable exergame protocol to prevent falls in the elderly population. The system is based on depth sensors and exergames. The experiments carried out with several seniors, in a day care center, make it possible to evaluate the usability and the efficiency of the system. The outcomes highlight the user-friendliness, the very good usability of the developed system and the significant enhancement of the elderly in maintaining a physical activity. The performance of the postural response is improved by an average of 80%.
Multiple-Criteria Decision-Making (MCDM) Techniques for Business Processes Information Management
Authors: Kazimieras Zavadskas Edmundas , Antucheviciene Jurgita , Chatterjee Prasenjit
Published: December 2018

Information management is a common paradigm in modern decision-making. A wide range of decision-making techniques have been proposed in the literature to model complex business processes. In this Special Issue, 16 selected and peer-reviewed original research articles contribute to business information management in various current real-world problems by proposing crisp or uncertain multiple-criteria decision-making (MCDM) models and techniques, mostly including multi-attribute decision-making (MADM) approaches in addition to a single paper proposing an interactive multi-objective decision-making (MODM) approach. The papers are mainly concentrated in three application areas: supplier selection and rational order allocation, the evaluation and selection of goods or facilities, and personnel selection/partner selection. A number of new approaches are proposed that are expected to attract great interest from the research community.
Linear Offset-Free Model Predictive Control in the Dynamic PLS Framework
Authors: Hou Ligang , Wu Ze , Jin Xin , Wang Yue
Published: December 2018

This work addresses the model predictive control (MPC) of the offset-free tracking problem in the dynamic partial least square (DyPLS) framework. Firstly, state space MPC based on the DyPLS is proposed. Then, two methods are proposed to solve the offset-free problem. One is to reform the state space model as a velocity form. Another is to augment the state space model with a disturbance model and estimate the mismatch between system output and model output with an estimator. Both methods use the system output as a feedback in the control scheme. Hence, the offset-free tracking is guaranteed, and unmeasured step disturbance can be rejected. The results of two simulations demonstrate the effectiveness of proposed methods.
Biochemical Effects of Petroselinum crispum (Umbellifereae) Essential Oil on the Pyrethroid Resistant Strains of Aedes aegypti (Diptera: Culicidae)
Authors: Intirach Jitrawadee , Junkum Anuluck , Lumjuan Nongkran , Chaithong Udom , Somboon Pradya , Jitpakdi Atchariya , Riyong Doungrat , Champakaew Danita , Muangmoon Roongtawan , Chansang Arpaporn , Pitasawat Benjawan
Published: December 2018

In ongoing screening research for edible plants, Petroselinum crispum essential oil was considered as a potential bioinsecticide with proven antimosquito activity against both the pyrethroid susceptible and resistant strains of Aedes aegypti. Due to the comparative mosquitocidal efficacy on these mosquitoes, this plant essential oil is promoted as an attractive candidate for further study in monitoring resistance of mosquito vectors. Therefore, the aim of this study was to evaluate the impact of P. crispum essential oil on the biochemical characteristics of the target mosquito larvae of Ae. aegypti, by determining quantitative changes of key enzymes responsible for xenobiotic detoxification, including glutathione-S-transferases (GSTs), α- and β-esterases (α-/β-ESTs), acetylcholinesterase (AChE), acid and alkaline phosphatases (ACP and ALP) and mixed-function oxidases (MFO). Three populations of Ae. aegypti, comprising the pyrethroid susceptible Muang Chiang Mai-susceptible (MCM-S) strain and the pyrethroid resistant Pang Mai Dang-resistant (PMD-R) and Upakut-resistant (UPK-R) strains, were used as test organisms. Biochemical study of Ae. aegypti larvae prior to treatment with P. crispum essential oil revealed that apart from AChE, the baseline activity of most defensive enzymes, such as GSTs, α-/β-ESTs, ACP, ALP and MFO, in resistant UPK-R or PMD-R, was higher than that determined in susceptible MCM-S. However, after 24-h exposure to P. crispum essential oil, the pyrethroid susceptible and resistant Ae. aegypti showed similarity in biochemical features, with alterations of enzyme activity in the treated larvae, as compared to the controls. An increase in the activity levels of GSTs, α-/β-ESTs, ACP and ALP was recorded in all strains of P. crispum oil-treated Ae. aegypti larvae, whereas MFO and AChE activity in these mosquitoes was decreased. The recognizable larvicidal capability on pyrethroid resistant Ae. aegypti, and the inhibitory effect on AChE and MFO, emphasized the potential of P. crispum essential oil as an attractive alternative application for management of mosquito resistance in current and future control programs.
Covering and Re-Covering the Heart: Development and Regeneration of the Epicardium
Authors: Cao Yingxi , Cao Jingli
Published: December 2018

The epicardium, a mesothelial layer that envelops vertebrate hearts, has become a therapeutic target in cardiac repair strategies because of its vital role in heart development and cardiac injury response. Epicardial cells serve as a progenitor cell source and signaling center during both heart development and regeneration. The importance of the epicardium in cardiac repair strategies has been reemphasized by recent progress regarding its requirement for heart regeneration in zebrafish, and by the ability of patches with epicardial factors to restore cardiac function following myocardial infarction in mammals. The live surveillance of epicardial development and regeneration using zebrafish has provided new insights into this topic. In this review, we provide updated knowledge about epicardial development and regeneration.
A Novel Mutation in the Stalk Domain of KIF5A Causes a Slowly Progressive Atypical Motor Syndrome
Authors: Filosto Massimiliano , Piccinelli Stefano , Palmieri Ilaria , Necchini Nicola , Valente Marialuisa , Zanella Isabella , Biasiotto Giorgio , Lorenzo Diego , Cereda Cristina , Padovani Alessandro
Published: December 2018

KIF5A encodes the heavy chain A of kinesin; A motor protein involved in motility functions within neuron. Mutations in the KIF5A N-terminal motor domain are known to cause SPG10; An autosomal dominant hereditary spastic paraplegia (HSP), as well as rare Charcot-Marie-Tooth disease 2 (CMT2) cases. Recently C-terminal cargo-binding tail domain mutations have been associated with an amyotrophic lateral sclerosis (ALS) phenotype. Here we describe a subject presenting with an atypical slowly progressive motor syndrome evolving over a period of 4 years; Characterized by walking difficulties; Muscle hypotrophy mainly involving upper limbs and pyramidal signs confined to the lower limbs. Electromyography demonstrated chronic neurogenic damage and active denervation while electroneurography showed slowly worsening axonal damage. We identified the novel heterozygote variant c.2341A>G in the exon 21 of the KIF5A gene resulting in the amino acid change p.Lys781Glu. The residue Lys781 is located within the terminal region of the stalk domain and is highly evolutionary conserved. Our findings confirm that mutations in KIF5A cause ALS-like phenotypes. However, the stalk domain mutation described here appears to result in an “intermediate” slowly progressive phenotype having aspects resembling ALS as well as HSP and axonal neuropathy. We suggest that KIF5A gene should be considered as a candidate gene in all atypical progressive motor syndromes.