bluuebunny/crossref_metadata_2025 huggingface dataset:
Discovery of Novel Chemical Series of OXA-48 β-Lactamase Inhibitors by High-Throughput Screening
Authors: Garofalo Barbara , Prati Federica , Buonfiglio Rosa , Coletta Isabella , D’Atanasio Noemi , Molteni Angela , Carettoni Daniele , Wanke Valeria , Pochetti Giorgio , Montanari Roberta , Capelli Davide , Milanese Claudio , Di Giorgio Francesco Paolo , Ombrato Rosella
Published: June 2021

The major cause of bacterial resistance to β-lactams is the production of hydrolytic β-lactamase enzymes. Nowadays, the combination of β-lactam antibiotics with β-lactamase inhibitors (BLIs) is the main strategy for overcoming such issues. Nevertheless, particularly challenging β-lactamases, such as OXA-48, pose the need for novel and effective treatments. Herein, we describe the screening of a proprietary compound collection against Klebsiella pneumoniae OXA-48, leading to the identification of several chemotypes, like the 4-ideneamino-4H-1,2,4-triazole (SC_2) and pyrazolo[3,4-b]pyridine (SC_7) cores as potential inhibitors. Importantly, the most potent representative of the latter series (ID2, AC50 = 0.99 μM) inhibited OXA-48 via a reversible and competitive mechanism of action, as demonstrated by biochemical and X-ray studies; furthermore, it slightly improved imipenem’s activity in Escherichia coli ATCC BAA-2523 β-lactam resistant strain. Also, ID2 showed good solubility and no sign of toxicity up to the highest tested concentration, resulting in a promising starting point for further optimization programs toward novel and effective non-β-lactam BLIs.
Interactions of Some Chemotherapeutic Agents as Epirubicin, Gemcitabine and Paclitaxel in Multicomponent Systems Based on Orange Essential Oil
Authors: Samide Adriana , Tutunaru Bogdan , Varut Renata-Maria , Oprea Bogdan , Iordache Simona
Published: June 2021

In order to anticipate the effect induced by a natural product on the chemical activity of medicines simultaneously administered, spontaneous interactions of certain cancer treatment drugs such as, epirubicin (EPR), gemcitabine (GCT), and paclitaxel (PTX) with limonene (LIM)—a natural compound extracted from orange peel and known as an anticancer agent—were investigated. To estimate the stability of the drugs over time, a current density of 50 mA cm−2 was applied as an external stimulus between two platinum electrodes immersed in hydrochloric acid solution containing ethyl alcohol/water in the volume ratio of 2/3, in the absence and presence of orange essential oil (limonene concentration of 95%). The concentration variation of chemotherapeutic agents over time was evaluated by UV-Vis spectrophotometry. Kinetic studies have shown a delay in the decomposition reaction of epirubicin and gemcitabine and a paclitaxel activity stimulation. Thus, in the presence of limonene, the epirubicin half-life increased from 46.2 min to 63 min, and from 6.2 min to 8.6 min in gemcitabine case, while for paclitaxel a decrease of half-life from 35.9 min to 25.8 min was determined. Therefore, certain drug-limonene interactions took place, leading to the emergence of molecular micro-assemblies impacting decomposition reaction of chemotherapeutics. To predict drug–limonene interactions, the Autodock 4.2.6 system was employed. Thus, two hydrophobic interactions and five π-alkyl interactions were established between EPR-LIM, the GCT-LIM connection involves four π-alkyl interactions, and the PTX-LIM bridges take place through three hydrophobic interactions and the one π-alkyl. Finally, the decomposition reaction mechanism of drugs was proposed.
Transdermal Delivery of Chemotherapeutics: Strategies, Requirements, and Opportunities
Authors: Neupane Rabin , Boddu Sai H. S. , Abou-Dahech Mariam Sami , Bachu Rinda Devi , Terrero David , Babu R. Jayachandra , Tiwari Amit K.
Published: June 2021

Chemotherapeutic drugs are primarily administered to cancer patients via oral or parenteral routes. The use of transdermal drug delivery could potentially be a better alternative to decrease the dose frequency and severity of adverse or toxic effects associated with oral or parenteral administration of chemotherapeutic drugs. The transdermal delivery of drugs has shown to be advantageous for the treatment of highly localized tumors in certain types of breast and skin cancers. In addition, the transdermal route can be used to deliver low-dose chemotherapeutics in a sustained manner. The transdermal route can also be utilized for vaccine design in cancer management, for example, vaccines against cervical cancer. However, the design of transdermal formulations may be challenging in terms of the conjugation chemistry of the molecules and the sustained and reproducible delivery of therapeutically efficacious doses. In this review, we discuss the nano-carrier systems, such as nanoparticles, liposomes, etc., used in recent literature to deliver chemotherapeutic agents. The advantages of transdermal route over oral and parenteral routes for popular chemotherapeutic drugs are summarized. Furthermore, we also discuss a possible in silico approach, Formulating for Efficacy™, to design transdermal formulations that would probably be economical, robust, and more efficacious.
Investigation on the Composition of Agarose–Collagen I Blended Hydrogels as Matrices for the Growth of Spheroids from Breast Cancer Cell Lines
Authors: Quarta Alessandra , Gallo Nunzia , Vergara Daniele , Salvatore Luca , Nobile Concetta , Ragusa Andrea , Gaballo Antonio
Published: June 2021

Three-dimensional (3D) cell culture systems mimic the structural complexity of the tissue microenvironment and are gaining increasing importance as they resemble the extracellular matrix (ECM)–cell and cell–cell physical interactions occurring in vivo. Several scaffold-based culture systems have been already proposed as valuable tools for large-scale production of spheroids, but they often suffer of poor reproducibility or high costs of production. In this work, we present a reliable 3D culture system based on collagen I-blended agarose hydrogels and show how the variation in the agarose percentage affects the physical and mechanical properties of the resulting hydrogel. The influence of the different physical and mechanical properties of the blended hydrogels on the growth, size, morphology, and cell motility of the spheroids obtained by culturing three different breast cancer cell lines (MCF-7, MDA-MB-361, and MDA-MB-231) was also evaluated. As proof of concept, the cisplatin penetration and its cytotoxic effect on the tumor spheroids as function of the hydrogel stiffness were also investigated. Noteworthily, the possibility to recover the spheroids from the hydrogels for further processing and other biological studies has been considered. This feature, in addition to the ease of preparation, the lack of cross-linking chemistry and the high reproducibility, makes this hydrogel a reliable biomimetic matrix for the growth of 3D cell structures.
Light- and Melanin Nanoparticle-Induced Cytotoxicity in Metastatic Cancer Cells
Authors: Gabriele Victoria R. , Mazhabi Robabeh M. , Alexander Natalie , Mukherjee Purna , Seyfried Thomas N. , Nwaji Njemuwa , Akinoglu Eser M. , Mackiewicz Andrzej , Zhou Guofu , Giersig Michael , Naughton Michael J. , Kempa Krzysztof
Published: June 2021

Melanin nanoparticles are known to be biologically benign to human cells for a wide range of concentrations in a high glucose culture nutrition. Here, we show cytotoxic behavior at high nanoparticle and low glucose concentrations, as well as at low nanoparticle concentration under exposure to (nonionizing) visible radiation. To study these effects in detail, we developed highly monodispersed melanin nanoparticles (both uncoated and glucose-coated). In order to study the effect of significant cellular uptake of these nanoparticles, we employed three cancer cell lines: VM-M3, A375 (derived from melanoma), and HeLa, all known to exhibit strong macrophagic character, i.e., strong nanoparticle uptake through phagocytic ingestion. Our main observations are: (i) metastatic VM-M3 cancer cells massively ingest melanin nanoparticles (mNPs); (ii) the observed ingestion is enhanced by coating mNPs with glucose; (iii) after a certain level of mNP ingestion, the metastatic cancer cells studied here are observed to die—glucose coating appears to slow that process; (iv) cells that accumulate mNPs are much more susceptible to killing by laser illumination than cells that do not accumulate mNPs; and (v) non-metastatic VM-NM1 cancer cells also studied in this work do not ingest the mNPs, and remain unaffected after receiving identical optical energy levels and doses. Results of this study could lead to the development of a therapy for control of metastatic stages of cancer.
Structure–Activity Relationship and Molecular Docking of a Kunitz-Like Trypsin Inhibitor, Kunitzin-AH, from the Skin Secretion of Amolops hainanensis
Authors: Chen Yuqing , Xi Xinping , Ma Chengbang , Zhou Mei , Chen Xiaoling , Ye Zhuming , Ge Lilin , Wu Qinan , Chen Tianbao , Wang Lei , Kwok Hang Fai
Published: June 2021

Kunitz-like trypsin inhibitors are one of the most noteworthy research objects owing to their significance in pharmacological studies, including anticarcinogenic activity, obesity regulation and anticoagulation. In the current study, a novel Kunitz-like trypsin inhibitor, Kunitzin-AH, was isolated from the skin secretion of Amolops hainanensis. The novel peptide displayed a modest trypsin inhibitory activity with the inhibitor constant (Ki) value of 1.18 ± 0.08 µM without inducing damage to healthy horse erythrocytes. Then, a series of shortened variants of Kunitzin-AH were designed by truncating a peptide loop and site mutation inside the loop to illustrate the structure–activity relationship of the trypsin inhibition function. Among the variants, a significant decrease was observed for the Cys-Cys loop domain, while the extension of an Arg at N-terminus (RCKAAFC) retained the inhibitory activity, indicating that the -RCK-motif is essential in forming the reactive domain for exerting the inhibitory activity. Furthermore, substitutions of Ala by hydrophobic or hydrophilic residues decreased the activity, indicating suitable steric hindrance provides convenience for the combination of trypsin. Additionally, the conformational simulation of the analogues processed with Chimera and Gromacs and further combination simulations between the peptides and trypsin conducted with HDOCK offered a potential opportunity for the natural trypsin inhibitory drug design. The truncated sequence, AH-798, may be a good replacement for the full-length peptide, and can be optimized via cyclization for further study.
Drug-Loaded Hydrogels for Intraocular Lenses with Prophylactic Action against Pseudophakic Cystoid Macular Edema
Authors: Toffoletto Nadia , Salema-Oom Madalena , Anguiano Igea Soledad , Alvarez-Lorenzo Carmen , Saramago Benilde , Serro Ana Paula
Published: June 2021

Pseudophakic cystoid macular edema (PCME), caused by chronic inflammation, is the most common cause of visual impairment in the medium-term after cataract surgery. Therefore, the prophylactic topical administration of combined steroidal and non-steroidal anti-inflammatory drugs is commonly done. Drug-eluting intraocular lenses (IOLs) gained interest as an efficient way to overcome the compliance issues related to the use of ocular drops without the need for additional surgical steps. The incorporation of functional monomers and molecular imprinting were herein applied to design hydrogels suitable as IOLs and able to co-deliver steroidal (dexamethasone sodium phosphate) and non-steroidal (bromfenac sodium) drugs. The incorporation of N-(2-aminopropyl) methacrylamide (APMA) increased the drug uptake and improved the in vitro release kinetics. Imprinting with bromfenac resulted in a decreased drug release due to permanent drug bonding, while imprinting with dexamethasone increased the amount of dexamethasone released after dual-drug loading. The application of a mathematical model to predict the in vivo drug release behavior suggests the feasibility of achieving therapeutic drug concentrations of bromfenac and dexamethasone in the aqueous humor for about 2 and 8 weeks, respectively, which is compatible with the current topical prophylaxis after cataract surgery.
The Importance of Propagule Dispersal in Maintaining Local Populations of Rare Algae on Complex Coastlines: Padina pavonica on the South Coast of England
Authors: Herbert Roger J.H. , Willis Jay , Baugh John
Published: June 2021

On dynamic coastlines, populations of protected algal species with poor dispersal might be especially vulnerable to infrequent recruitment events and local extinction. As a model, we here consider the dispersal of the alga Padina pavonica from the largest remaining and physically isolated enclaves on the south coast of England. A bio-physical model was used to investigate the likely importance of local propagule dispersal in maintaining populations. Dispersal kernels that simulate the position of propagules at different time steps over 5 days were examined from five release sites. Exceptionally steep declines in model propagule density were observed over the first few hours from release, yet over the first day, 75–85% of model propagules remained close to their source but had not reached other enclaves. After five days, the dispersal from source populations ranged from 0 to 50 km, with only ~5% remaining within the source 1 km2 area. Although distances of modelled propagule dispersal might be adequate for maintaining a regional population network, vegetative perrenation also appears to be important for persistence of P. pavonica. For rare and protected species on isolated and energetic coastlines, local conservation efforts, rather than a reliance on a wider meta-population network, remain very important to ensure long-term protection and survival.
Pollen Germination and Pollen Tube Growth in Gymnosperms
Authors: Breygina Maria , Klimenko Ekaterina , Schekaleva Olga
Published: June 2021

Pollen germination and pollen tube growth are common to all seed plants, but these processes first developed in gymnosperms and still serve for their successful sexual reproduction. The main body of data on the reproductive physiology, however, was obtained on flowering plants, and one should be careful to extrapolate the discovered patterns to gymnosperms. In recent years, physiological studies of coniferous pollen have been increasing, and both the features of this group and the similarities with flowering plants have already been identified. The main part of the review is devoted to physiological studies carried out on conifer pollen. The main properties and diversity of pollen grains and pollination strategies in gymnosperms are described.
Nitrogen and Sulphur Fertilisation for Marketable Yields of Cabbage (Brassica oleracea L. var. Capitata), Leaf Nitrate and Glucosinolates and Nitrogen Losses Studied in a Field Experiment in Central Slovenia
Authors: Kacjan Maršić Nina , Može Ksenija Sinkovič , Mihelič Rok , Nečemer Marijan , Hudina Metka , Jakopič Jerneja
Published: June 2021

A field trial of white cabbage (Brassica oleracea var. Capitata L.) was carried out under the humid temperate climate conditions in Central Slovenia to investigate the effects of calcium ammonium nitrate (0, 180 and 240 kg N ha−1) and gypsum (0 and 40 kg S ha−1) fertilisation on yield, yield quality (nitrate, glucosinolate levels and glucosinolate profile) and nitrogen use efficiency. The highest marketable yield, dry matter yield and nitrogen uptake were obtained at the highest nitrogen fertilisation rate when in combination with sulphur. For this treatment, the nitrogen surplus in the soil after harvesting was lower than for the same nitrogen fertilisation without sulphur application. For the combination N240S40, the sulphur addition significantly increased nitrogen use efficiency, which resulted in reduced nitrate content in the cabbage heads. The chemical forms of glucosinolates showed that 80–85% were aliphatic glucosinolates with the remainder as the indole group. For the aliphatic glucosinolates, significant interactions between nitrogen and sulphur fertilisations were reflected in increased levels of progoitrin and glucoiberin when sulphur was applied at the lower nitrogen fertilisation rates. For the indole group, the levels of glucobrassicin and the indole group itself decreased at higher nitrogen fertilisation rates, independent of sulphur fertilisation.
Phytocannabinoids Biosynthesis in Angiosperms, Fungi, and Liverworts and Their Versatile Role
Authors: Arif Yamshi , Singh Priyanka , Bajguz Andrzej , Hayat Shamsul
Published: June 2021

Phytocannabinoids are a structurally diverse class of bioactive naturally occurring compounds found in angiosperms, fungi, and liverworts and produced in several plant organs such as the flower and glandular trichrome of Cannabis sativa, the scales in Rhododendron, and oil bodies of liverworts such as Radula species; they show a diverse role in humans and plants. Moreover, phytocannabinoids are prenylated polyketides, i.e., terpenophenolics, which are derived from isoprenoid and fatty acid precursors. Additionally, targeted productions of active phytocannabinoids have beneficial properties via the genes involved and their expression in a heterologous host. Bioactive compounds show a remarkable non-hallucinogenic biological property that is determined by the variable nature of the side chain and prenyl group defined by the enzymes involved in their biosynthesis. Phytocannabinoids possess therapeutic, antibacterial, and antimicrobial properties; thus, they are used in treating several human diseases. This review gives the latest knowledge on their role in the amelioration of abiotic (heat, cold, and radiation) stress in plants. It also aims to provide synthetic and biotechnological approaches based on combinatorial biochemical and protein engineering to synthesize phytocannabinoids with enhanced properties.
Stomatal Traits and Barley (Hordeum vulgare L.) Forage Yield in Drought Conditions of Northeastern Mexico
Authors: Rahim Foroughbakhch Pournavab , María Alejandra Torres Tapia , Víctor Manuel Zamora Villa , José Elías Treviño Ramirez , Maginot Ngangyo Heya
Published: June 2021

Infrared technology is a practical, fast, non-destructive method that helps in forecasting plant development and can be used to select physiological traits, instead of other methodologies that require more time and breeding efforts. According to the statistical analyses and the relationship between the direct and indirect effects of the variables, this technology could serve as the basis for implementing a genotype selection methodology. Awnless barley was assessed in a randomized block design with three replicates in two crop seasons at Mexico’s northeastern region. Two samplings were carried out during crop development: at 75 and 90 days after sowing. The infrared and stomatal sensors were used to identify the direct and indirect effects of stomata’s traits on dry forage yield. The data were analyzed in a subdivided plot design, using mean comparison tests, correlation coefficients and path analyses, finding significant differences (p < 0.05) among localities and among samplings. Dry forage yield was significant and positively correlated with plant height (r = 0.578) and canopy temperature (r = 0.724), and negatively correlated with the leaf upper side stomatal width (r = −0.409) and the leaf lower side stomatal width (r = −0.641), chlorophyll content and vegetation index. Temperature, chlorophyll, density and leaf lower side stomatal index had the strongest direct effects on yield. Therefore, the infrared technology appears as a way to select high yielding awnless forage barley, to obtain the correlation, the positive direct effect of temperature and the negative effect of chlorophyll. Due to their direct effects, low density and low leaf underside stomatal indexes can also help in the indirect selection of higher yielding forage barley genotypes.
Soil Moisture Levels Affect the Anatomy and Mechanical Properties of Basil Stems (Ocimum basilicum L.)
Authors: Driesen Elisa , De Proft Maurice , Saeys Wouter
Published: June 2021

As plants would benefit from adjusting and optimizing their architecture to changing environmental stimuli, ensuring a strong and healthy plant, it was hypothesized that different soil moisture levels would affect xylem and collenchyma development in basil (Ocimum basilicum L. cv. Marian) stems. Four different irrigation set-points (20, 30, 40 and 50% VWC), corresponding respectively to pF values of 1.95, 1.65, 1.30 and 1.15, were applied. Basil plants grown near the theoretical wilting point (pF 2) had a higher xylem vessel frequency and lower mean vessel diameter, promoting water transport under drought conditions. Cultivation at low soil moisture also impacted the formation of collenchyma in the apical stem segments, providing mechanical and structural support to these fast-growing stems and vascular tissues. The proportion of collenchyma area was significantly lower for the pF1.15 treatment (9.25 ± 3.24%) compared to the pF1.95 and pF1.30 treatments (16.04 ± 1.83% and 13.28 ± 1.38%, respectively). Higher fractions of collenchyma resulted in a higher mechanical stem strength against bending. Additionally, tracheids acted as the major support tissues in the basal stem segments. These results confirm that the available soil moisture impacts mechanical stem strength and overall plant quality of basil plants by impacting xylem and collenchyma development during cultivation, ensuring sufficient mechanical support to the fast-growing stem and to the protection of the vascular tissues. To our knowledge, this study is the first to compare the mechanical and anatomical characteristics of plant stems cultivated at different soil moisture levels.
Environmental Hazards of Giant Reed (Arundo donax L.) in the Macaronesia Region and Its Characterisation as a Potential Source for the Production of Natural Fibre Composites
Authors: Suárez Luis , Castellano Jessica , Romero Francisco , Marrero María Dolores , Benítez Antonio Nizardo , Ortega Zaida
Published: June 2021

This paper summarises the results obtained from the characterisation of giant reed (Arundo donax L.) plant and fibres. The research is part of a project developed in the Macaronesia region, of which the aim is to demonstrate the feasibility of using biomass from invasive plant species in the composites sector as a way of financing control campaigns and habitats conservation labours. An experimental procedure for the extraction of fibre bundles from this plant was developed, and the material obtained was characterised in terms of chemical composition, thermogravimetry and infrared spectra to evaluate its potential application in the production of polymeric composite materials as a strategy for the valorisation of residual biomass from this invasive species in Macaronesia. Thermoplastic matrix composites with fibre content up to 40 wt.% were produced and their mechanical properties under tensile, flexural and impact loading were determined. No references on the preparation of composite materials with polyolefin matrices and giant reed fibres have been found. Results obtained from mechanical tests show a good performance of the manufactured composites, with a significant increase in both flexural and tensile stiffness; the flexural modulus is almost tripled for PE-based composites and rises to 88% with respect to PP matrix. The ultimate flexural strength and the tensile and flexural yield strength are kept at acceptable values compared to neat polymer materials, although ultimate tensile strength and impact resistance are significantly affected when natural fibres are added.
Fabrication of Hybrid Nanofibers from Biopolymers and Poly (Vinyl Alcohol)/Poly (ε-Caprolactone) for Wound Dressing Applications
Authors: Alven Sibusiso , Aderibigbe Blessing Atim
Published: June 2021

The management of chronic wounds is challenging. The factors that impede wound healing include malnutrition, diseases (such as diabetes, cancer), and bacterial infection. Most of the presently utilized wound dressing materials suffer from severe limitations, including poor antibacterial and mechanical properties. Wound dressings formulated from the combination of biopolymers and synthetic polymers (i.e., poly (vinyl alcohol) or poly (ε-caprolactone) display interesting properties, including good biocompatibility, improved biodegradation, good mechanical properties and antimicrobial effects, promote tissue regeneration, etc. Formulation of these wound dressings via electrospinning technique is cost-effective, useful for uniform and continuous nanofibers with controllable pore structure, high porosity, excellent swelling capacity, good gaseous exchange, excellent cellular adhesion, and show a good capability to provide moisture and warmth environment for the accelerated wound healing process. Based on the above-mentioned outstanding properties of nanofibers and the unique properties of hybrid wound dressings prepared from poly (vinyl alcohol) and poly (ε-caprolactone), this review reports the in vitro and in vivo outcomes of the reported hybrid nanofibers.
Design of Dopant and Lead-Free Novel Perovskite Solar Cell for 16.85% Efficiency
Authors: Moiz Syed Abdul , Alahmadi Ahmed N. M.
Published: June 2021

Halide based perovskite offers numerous advantages such as high-efficiency, low-cost, and simple fabrication for flexible solar cells. However, long-term stability as well as environmentally green lead-free applications are the real challenges for their commercialization. Generally, the best reported perovskite solar cells are composed of toxic lead (Pb) and unstable polymer as the absorber and electron/hole-transport layer, respectively. Therefore, in this study, we proposed and simulated the photovoltaic responses of lead-free absorber such as cesium titanium (IV) bromide, Cs2TiBr6 with dopant free electron phenyl-C61-butyric acid methyl ester (PCBM), and dopant free hole transport layer N,N′-Di(1-naphthyl)-N,N′-diphenyl-(1,1′-biphenyl)-4,4′-diamine (NPB) for the Ag/BCP/PCBM/Cs2TiBr6/NPB/ITO based perovskite solar cell. After comprehensive optimization of each layer through vigorous simulations with the help of software SCAPS 1D, it is observed that the proposed solar cell can yield maximum power-conversion efficiency up to 16.85%. This efficiency is slightly better than the previously reported power-conversion efficiency of a similar type of perovskite solar cell. We believe that the outcome of this study will not only improve our knowledge, but also triggers further investigation for the dopant and lead-free perovskite solar cell.
Development of Active Packaging to Extend the Shelf Life of Agaricus bisporus by Using Plasma Technology
Authors: Chang Chao-Kai , Cheng Kuan-Chen , Hou Chih-Yao , Wu Yi-Shan , Hsieh Chang-Wei
Published: June 2021

In this study, a preservation package that can extend the shelf life of Agaricus bisporus was developed using plasma modification combined with low-density polyethylene (LDPE), collagen (COL), and carboxymethyl cellulose (CMC). Out results showed that the selectivity of LDPE to gas can be controlled by plasma modification combined with coating of different concentrations of CMC and COL. Packaging test results applied to A. bisporus showed that 3% and 5% of CMC and COL did not significantly inhibit polyphenol oxidase and β-1,3-glucanase, indicating no significant effect on structural integrity and oxidative browning. The use of 0.5% and 1.0% CMC and COL can effectively inhibit the polyphenol oxidase and β-1,3-glucanase activity of A. bisporus, leading to improved effects in browning inhibition and structural integrity maintenance. P-1.0COL can effectively maintain gas composition in the package (carbon dioxide: 10–15% and oxygen: 8–15%) and catalase activity during storage, thereby reducing the oxidative damage caused by respiration of A. bisporus. The current study confirmed that the use of plasma modification technology combined with 1.0% COL can be used in preservation packaging by regulating the respiration of A. bisporus, thus extending its shelf life from 7 to 21 days.
Theoretical Estimation of Evaporation Heat in Paper Drying Process Based on Drying Curve
Authors: Chen Xiaobin , Zheng Qifu , Dong Yunyuan
Published: June 2021

At present, the theoretical estimation of paper web’s evaporation heat is based on sorption isothermals. The measuring conditions are harsh, and the test speed is slow. This paper attempts to explore a theoretical method which can quickly determine the evaporation heat of paper web. During the new method, based on the measurement of the paper drying curve, the theoretical estimation model of paper evaporation heat was obtained by deducing the mechanism of heat and mass transfer. Compared with the traditional method based on sorption isothermals, the new model based on the drying curve has some advantages in measurement speed and easy access to basic data. Finally, the paper verifies the reliability of the model from two application scenarios of the laboratory and production line.
The Osteogenic Function of Danggui Buxue Tang, a Herbal Decoction Containing Astragali Radix and Angelicae Sinensis Radix, Is Optimized by Boiling the Two Herbs Together: Signaling Analyses Revealed by Systems Biology
Authors: Kwan Kenneth , Wong Tin , Yu Anna , Dong Tina , Lam Henry , Tsim Karl
Published: June 2021

The therapeutic efficacy of a herbal mixture, being multi-target, multi-function and multi-pathway, is the niche of traditional Chinese medicine (TCM). Systems biology can dissect the network of signaling mechanisms in a complex biological system. In preparing TCM decoctions, the boiling of herbs together in water is a common practice; however, the rationale of this specific preparation has not been fully revealed. An approach of mass-spectrometry-based multi-omics was employed to examine the profiles of the cellular pathways, so as to understand the pharmacological efficacy of Danggui Buxue Tang (DBT), a Chinese herbal mixture containing Astragali Radix and Angelicae Sinensis Radix, in cultured rat osteoblasts and mesenchymal stem cells. The results, generated from omics analyses, were compared from DBT-treated osteoblasts to those of treating the herbal extract by simple mixing of extracts from Astragali Radix and Angelicae Sinensis Radix, i.e., herbal mixture without boiling together. The signaling pathways responsible for energy metabolism and amino acid metabolism showed distinct activation, as triggered by DBT, in contrast to simple mixing of two herbal extracts. The result supports that boiling the herbs together is designed to maximize the osteoblastic function of DBT, such as in energy and lipid metabolism. This harmony of TCM formulation, by having interactive functions of two herbs during preparation, is being illustrated. The systems biology approach provides new and essential insights into the synergy of herbal preparation. Well-defined multiple targets and multiple pathways in different levels of omics are the key to modernizing TCM.
Are International Human Rights Organizations Effective in Protecting Religious Freedom?
Authors: Zhang Lihui
Published: June 2021

This paper examines whether international human rights organizations (HROs) influence the protection of religious freedom, and whether the effect is different on the protection of religious rights for institutions versus individuals. This study not only reveals the institutional and individual dimensions of religious restrictions with an exploratory factor analysis, but also uses fixed effects models to analyze cross-national time-series data covering 1990–2003. The results indicate that the domestic presence of HROs has a positive effect on reducing both aggregate religious restrictions and the two dimensions of religious restrictions.
Laryngeal Paralysis Recovered Two Years after a Head Trauma by Growth Hormone Treatment and Neurorehabilitation
Authors: Guerra Joaquín , Lema Hortensia , Agra Carlos , Martínez Pedro , Devesa Jesús
Published: June 2021

The aim of this study was to describe the cognitive and speech results obtained after growth hormone (GH) treatment and neurorehabilitation in a man who suffered a traumatic brain injury (TBI). Seventeen months after the accident, the patient was treated with growth hormone (GH), together with neurostimulation and speech therapy. At admission, the flexible laryngoscopy revealed that the left vocal cord was paralyzed, in the paramedian position, a situation compatible with a recurrent nerve injury. Clinical and rehabilitation assessments revealed a prompt improvement in speech and cognitive functions and, following completion of treatment, endoscopic examination showed recovery of vocal cord mobility. These results, together with previous results from our group, indicate that GH treatment is safe and effective for helping neurorehabilitation in chronic speech impairment due to central laryngeal paralysis, as well as impaired cognitive functions.
An Efficient Multi-Sensor Remote Sensing Image Clustering in Urban Areas via Boosted Convolutional Autoencoder (BCAE)
Authors: Rahimzad Maryam , Homayouni Saeid , Alizadeh Naeini Amin , Nadi Saeed
Published: June 2021

High-resolution urban image clustering has remained a challenging task. This is mainly because its performance strongly depends on the discrimination power of features. Recently, several studies focused on unsupervised learning methods by autoencoders to learn and extract more efficient features for clustering purposes. This paper proposes a Boosted Convolutional AutoEncoder (BCAE) method based on feature learning for efficient urban image clustering. The proposed method was applied to multi-sensor remote-sensing images through a multistep workflow. The optical data were first preprocessed by applying a Minimum Noise Fraction (MNF) transformation. Then, these MNF features, in addition to the normalized Digital Surface Model (nDSM) and vegetation indexes such as Normalized Difference Vegetation Index (NDVI) and Excess Green (ExG(2)), were used as the inputs of the BCAE model. Next, our proposed convolutional autoencoder was trained to automatically encode upgraded features and boost the hand-crafted features for producing more clustering-friendly ones. Then, we employed the Mini Batch K-Means algorithm to cluster deep features. Finally, the comparative feature sets were manually designed in three modes to prove the efficiency of the proposed method in extracting compelling features. Experiments on three datasets show the efficiency of BCAE for feature learning. According to the experimental results, by applying the proposed method, the ultimate features become more suitable for clustering, and spatial correlation among the pixels in the feature learning process is also considered.
Assessment of the Reprocessed Suomi NPP VIIRS Enterprise Cloud Mask Product
Authors: Lin Lin , Hao Xianjun , Zhang Bin , Zou Cheng-Zhi , Cao Changyong
Published: June 2021

The Visible Infrared Imaging Radiometer Suite (VIIRS) onboard the Suomi National Polar-orbiting Partnership (S-NPP) satellite continually provides global observations used to retrieve over 20 VIIRS Environmental Data Record (EDR) products. Among them, the cloud mask product is essential for many other VIIRS EDR products such as aerosols, ocean color, and active fire. The reprocessed S-NPP VIIRS Sensor Data Record (SDR) data produced by NOAA/Center for Satellite Applications and Research (STAR) have shown improved stability and consistency. Recently, the VIIRS Enterprise Cloud Mask (ECM) has been reprocessed using the reprocessed VIIRS SDR data. This study assesses the reprocessed ECM product by comparing the reprocessed cloud mask types and cloud probability with those from the operational VIIRS ECM product. It found that the overall differences are small. Most of the discrepancies occur between neighboring types at the cloud edge. These findings help lay the foundation for the user community to understand the reprocessed ECM product. In addition, due to the better quality of the reprocessed VIIRS SDR data that are utilized to generate the reprocessed ECM product, it is expected that the reprocessed ECM product will have better stability and consistency compared to the operational ECM products. Therefore, the reprocessed ECM product is a useful benchmark for the user community.
Improved NOAA-20 Visible Infrared Imaging Radiometer Suite Day/Night Band Image Quality by Upgraded Gain Calibration
Authors: Gu Yalong , Blonski Slawomir , Wang Wenhui , Uprety Sirish , Choi Taeyoung , Shao Xi , Zhang Bin , Cao Changyong
Published: June 2021

Due to complex radiometric calibration, the imagery collected by the Day/Night Band (DNB) of the Visible Infrared Imaging Radiometer Suite (VIIRS) onboard the Suomi National Polar Partnership (Suomi-NPP) and the NOAA-20 follow-on satellite is subject to artifacts such as striping, which eventually affect Earth remote sensing applications. Through comprehensive analysis using the NOAA-20 VIIRS DNB prelaunch-test and on-orbit data, it is revealed that the striping results from flaws in the calibration process. In particular, a discrepancy between the low-gain stage (LGS) Earth view (EV) gain and the onboard calibrator solar diffuser view gain makes the operational LGS gain coefficients of a few aggregation modes and detectors biased. Detector nonlinearity at low radiance level also induces errors to the mid-gain stage (MGS) and high-gain stage (HGS) gain through the biased gain ratios. These systematic errors are corrected by scaling the operational LGS gains using the factors derived from the NOAA-20 VIIRS DNB prelaunch test data and by adopting linear regression for evaluating the gain ratios. Striping in the NOAA-20 VIIRS DNB imagery is visibly reduced after the upgraded gain calibration process was implemented in the operational calibration.
Self-Attention in Reconstruction Bias U-Net for Semantic Segmentation of Building Rooftops in Optical Remote Sensing Images
Authors: Chen Ziyi , Li Dilong , Fan Wentao , Guan Haiyan , Wang Cheng , Li Jonathan
Published: June 2021

Deep learning models have brought great breakthroughs in building extraction from high-resolution optical remote-sensing images. Among recent research, the self-attention module has called up a storm in many fields, including building extraction. However, most current deep learning models loading with the self-attention module still lose sight of the reconstruction bias’s effectiveness. Through tipping the balance between the abilities of encoding and decoding, i.e., making the decoding network be much more complex than the encoding network, the semantic segmentation ability will be reinforced. To remedy the research weakness in combing self-attention and reconstruction-bias modules for building extraction, this paper presents a U-Net architecture that combines self-attention and reconstruction-bias modules. In the encoding part, a self-attention module is added to learn the attention weights of the inputs. Through the self-attention module, the network will pay more attention to positions where there may be salient regions. In the decoding part, multiple large convolutional up-sampling operations are used for increasing the reconstruction ability. We test our model on two open available datasets: the WHU and Massachusetts Building datasets. We achieve IoU scores of 89.39% and 73.49% for the WHU and Massachusetts Building datasets, respectively. Compared with several recently famous semantic segmentation methods and representative building extraction methods, our method’s results are satisfactory.
Disrupted Care Continuity: Testing Associations between Social Networks and Transition Success for Children with Autism
Authors: McGhee Hassrick Elizabeth , Shih Wendy , Nuske Heather J. , Vejnoska Sarah F. , Hochheimer Samantha , Linares Deborah E. , Ventimiglia Jonas , Carley Kathleen M. , Stahmer Aubyn C. , Smith Tristram , Mandell David , Kasari Connie
Published: June 2021

Children with autism situated in lower income families often receive intensive educational interventions as their primary form of treatment, due to financial barriers for community interventions. However, the continuity of care can be disrupted by school transitions. The quality of social relationships during the transition to a new school among parents, school staff and community providers, called the team-around-the-child (TAC), can potentially buffer a child with autism from the adverse effects caused by care disruptions. Qualities of social relationships, including trust and collaborative problem solving, can be measured using social network analysis. This study investigates if two different types of TAC relationships, defined as (1) the level of trust among team members and (2) the degree of collaborative problem solving among team members, are associated with perceived successful transitions for children with autism from lower income families. Findings suggested that TAC trust is significantly associated with the outcome of transition success for children with autism immediately post-transition.
Implications for Sustainability of the Joint Application of Bioeconomy and Circular Economy: A Worldwide Trend Study
Authors: Abad-Segura Emilio , Batlles-delaFuente Ana , González-Zamar Mariana-Daniela , Belmonte-Ureña Luis Jesús
Published: June 2021

The joint application of bioeconomy (BE) and circular economy (CE) promotes the sustainable use of natural resources, since by applying a systemic approach, it improves the efficiency of these resources and reduces the impact on the environment. Both strategies, which belong to the area of green economy, provide a global and integrated approach towards environmental sustainability, as regards the extraction of biological materials, the protection of biodiversity and even the primary function of food production in agriculture. The objective was to analyze the implications for sustainability of BE and CE joint application. A systematic and bibliometric review has been applied to a sample of 1961 articles, selected from the period 2004–May 2021. A quantitative and qualitative advance is observed in this field of study. The expansion of scientific production is due to its multidisciplinary nature, since it implies technical, environmental and economic knowledge. The main contribution of this study is to understand the state of research on the implications for sustainability that BE and CE have when combined, in relation to their evolution, the scientific collaboration between the main driving agents, and the identification of the main lines of research developed.
Comparative Study of Computational Models for Reducing Air Pollution through the Generation of Negative Ions
Authors: Ortiz-Grisales Paola , Patiño-Murillo Julián , Duque-Grisales Eduardo
Published: June 2021

Today, air quality is one of the global concerns that governments are facing. One of the main air pollutants is the particulate matter (PM) which affects human health. This article presents the modeling of a purification system by means of negative air ions (NAIs) for air pollutant removal, using computational intelligence methods. The system uses a high-voltage booster output to ionize air molecules from stainless steel electrodes; its particle-capturing efficiency reaches up to 97%. With two devices (5 cm × 2 cm × 2.5 cm), 2 trillion negative ions are produced per second, and the particulate matter (PM 2.5) can be reduced from 999 to 0 mg/m3 in a period of approximately 5 to 7 minutes (in a 40 cm × 40 cm × 40 cm acrylic chamber). This negative ion generator is a viable and sustainable alternative to reduce polluting emissions, with beneficial effects on human health.
Toward Sociocultural Indicators of Sustainable Healthy Diets
Authors: Nicholls Jill , Drewnowski Adam
Published: June 2021

Balancing the social, economic and environmental priorities for public health is at the core of the United Nations (UN) approaches to sustainable development, including the Sustainable Development Goals (SDGs). The four dimensions of sustainable diets are often presented as health, society, economics, and the environment. Although sustainable diet research has focused on health and the environment, the social and economic dimensions of sustainable diets and food systems should not be forgotten. Some research priorities and sociocultural indicators for sustainable healthy diets and food systems are outlined in this report. The present goal is to improve integration of the social dimension into research on food and nutrition security.
Urban Shopping Malls and Sustainability Approaches in Chilean Cities: Relations between Environmental Impacts of Buildings and Greenwashing Branding Discourses
Authors: De Simone Liliana , Pezoa Mario
Published: June 2021

This article seeks to discuss the recent discrepancies between the environmental effects of large retail buildings located in urban centers and the media and corporate discourses that these retailers have on the environment and sustainability to the public and to local authorities. By using mixed methods of quantitative and qualitative data analysis of two of the biggest shopping centers in Santiago de Chile, this exploratory research seeks to inaugurate an interdisciplinary academic discussion on the relevance of comparing the media discourses about sustainability issued by urban developers and the physical effects that massive private buildings create in their surroundings. By using the retail resilience theoretical framework, this research seeks to understand the disruption in retail systems, both from socio-economic and environmental indicators. Comparing the environmentally sensitive discourses promoted by Chilean retailers in advertising campaigns and corporate speeches with the environmental effects that large retail buildings brought to the surrounding areas in terms of urban deforestation and increasing superficial temperature, this paper probes that greenwashing campaign are not only used to impact corporate legitimacy with consumers and investors but also to deliberately diminish the environmental responsibilities of private developers in vulnerable urban areas.
Effect of Mangrove Biochar Residue Amended Shrimp Pond Sediment on Nitrogen Adsorption and Leaching
Authors: Be Sokkeang , Vinitnantharat Soydoa , Pinisakul Anawat
Published: June 2021

Mangrove biochar residue was used for nitrogen adsorption and retention in sediment, which is beneficial for plant germination. The present study investigated the effect of contact time (5–360 min), biochar dosage (0.2–2 g L−1), pH (5–6), and initial concentration (2–10 mg L−1) on NH4+-N and NO3−-N adsorption. Three different adsorption isotherm models were used to fit the experimental data. Column leaching experiments were conducted to investigate the effect of biochar with sediment from a shrimp pond on nitrogen leaching at varying biochar dosages (0–8% w/w). The results showed that the maximum percentage of both NH4+-N and NO3−-N adsorption was achieved at an equilibrium contact time of 240 min, with an adsorbent dosage of 2 g L−1, and pH at 5.5 and 5, respectively. The adsorption of NH4+-N and NO3−-N were fitted to the Freundlich model and the adsorption process followed the physisorption and ion exchange. The addition of 8% biochar reduced both cumulative water volume and nitrogen leaching from the sediment. The biochar amendment increased the relative abundance of nitrifying and denitrifying bacteria in the sediment. This result suggested that biochar amended with sediment could be useful for nitrogen loss reduction.