bluuebunny/crossref_metadata_2025 huggingface dataset:
Fc-Independent Protection from SARS-CoV-2 Infection by Recombinant Human Monoclonal Antibodies
Authors: Noy-Porat Tal , Edri Avishay , Alcalay Ron , Makdasi Efi , Gur David , Aftalion Moshe , Evgy Yentl , Beth-Din Adi , Levy Yinon , Epstein Eyal , Radinsky Olga , Zauberman Ayelet , Lazar Shirley , Yitzhaki Shmuel , Marcus Hadar , Porgador Angel , Rosenfeld Ronit , Mazor Ohad
Published: November 2021

The use of passively-administered neutralizing antibodies is a promising approach for the prevention and treatment of SARS-CoV-2 infection. Antibody-mediated protection may involve immune system recruitment through Fc-dependent activation of effector cells and the complement system. However, the role of Fc-mediated functions in the efficacious in-vivo neutralization of SARS-CoV-2 is not yet clear, and it is of high importance to delineate the role this process plays in antibody-mediated protection. Toward this aim, we have chosen two highly potent SARS-CoV-2 neutralizing human monoclonal antibodies, MD65 and BLN1 that target distinct domains of the spike (RBD and NTD, respectively). The Fc of these antibodies was engineered to include the triple mutation N297G/S298G/T299A that eliminates glycosylation and the binding to FcγR and to the complement system activator C1q. As expected, the virus neutralization activity (in-vitro) of the engineered antibodies was retained. To study the role of Fc-mediated functions, the protective activity of these antibodies was tested against lethal SARS-CoV-2 infection of K18-hACE2 transgenic mice, when treatment was initiated either before or two days post-exposure. Antibody treatment with both Fc-variants similarly rescued the mice from death reduced viral load and prevented signs of morbidity. Taken together, this work provides important insight regarding the contribution of Fc-effector functions in MD65 and BLN1 antibody-mediated protection, which should aid in the future design of effective antibody-based therapies.
Wastewater-Based Surveillance of Antibiotic Resistance Genes Associated with Tuberculosis Treatment Regimen in KwaZulu Natal, South Africa
Authors: Mtetwa Hlengiwe N. , Amoah Isaac D. , Kumari Sheena , Bux Faizal , Reddy Poovendhree
Published: November 2021

Essential components of public health include strengthening the surveillance of infectious diseases and developing early detection and prevention policies. This is particularly important for drug-resistant tuberculosis (DR-TB), which can be explored by using wastewater-based surveillance. This study aimed to use molecular techniques to determine the occurrence and concentration of antibiotic-resistance genes (ARGs) associated with tuberculosis (TB) resistance in untreated and treated wastewater. Raw/untreated and treated (post-chlorination) wastewater samples were taken from three wastewater treatment plants (WWTPs) in South Africa. The ARGs were selected to target drugs used for first- and second-line TB treatment. Both conventional polymerase chain reaction (PCR) and the more advanced droplet digital PCR (ddPCR) were evaluated as surveillance strategies to determine the distribution and concentration of the selected ARGs. The most abundant ARG in the untreated wastewater was the rrs gene, associated with resistance to the aminoglycosides, specifically streptomycin, with median concentration ranges of 4.69–5.19 log copies/mL. In contrast, pncA gene, associated with resistance to the TB drug pyrazinamide, was the least detected (1.59 to 2.27 log copies/mL). Resistance genes associated with bedaquiline was detected, which is a significant finding because this is a new drug introduced in South Africa for the treatment of multi-drug resistant TB. This study, therefore, establishes the potential of molecular surveillance of wastewater for monitoring antibiotic resistance to TB treatment in communities.
Application of Heating on the Antioxidant and Antibacterial Properties of Malaysian and Australian Stingless Bee Honey
Authors: Mat Ramlan Nurul Ainaa Farhanah , Md Zin Aina Syahirah , Safari Nur Fatihah , Chan Kim Wei , Zawawi Norhasnida
Published: November 2021

In the honey industry, heat treatments are usually applied to maintain honey’s quality and shelf life. Heat treatment is used to avoid crystallisation and allow the easy use of honey, but treatment with heat might affect the antioxidant and antibacterial activities, which are the immediate health effects of honey. This study will determine the effect of heat treatment on Malaysian and Australian stingless bee honey (SBH) produced by the common bee species in both countries. Eighteen honey samples were subjected to heat at 45 °C, 55 °C and 65 °C for one hour and subsequently analysed for their total phenolic content (TPC), total flavonoid content (TFC), DPPH radical scavenging activity, ferric reducing antioxidant power (FRAP) and minimum inhibitory concentration (MIC). The results show that all samples had high TPC, TFC and antioxidant activities before the treatment. The heat treatments did not affect (p < 0.05) the TPC, TFC and antioxidant activities in most samples, but did inhibit the antibacterial activities consistently in most of the samples, regardless of the bee species and country of origin. This study also confirms a strong correlation between TPC and TFC with FRAP activities for the non-heated and heated honey samples (p < 0.05). Other heat-sensitive bioactive compounds in SBH should be measured to control the antibacterial properties present.
Hydropinotherapy with Sulphurous Mineral Water as Complementary Treatment to Improve Glucose Metabolism, Oxidative Status, and Quality of Life
Authors: Costantino Maria , Conti Valeria , Corbi Graziamaria , Filippelli Amelia
Published: November 2021

Hydropinotherapy is a salus per aquam (Spa) treatment suitable as a complementary approach to treat several diseases, which strongly affect the quality of life (QoL). Hydropinotherapy with sulphurous mineral water exerts benefits thanks to components, such as hydrogen sulphide, which is considered mainly responsible for antioxidant and hypoglycaemic effects. Such properties, linked from each other, could favour an improvement in patients’ QoL. However, data on humans are scarce. This study aimed to investigate whether a cycle of sulphurous hydropinotherapy was able to modify plasma levels of glucose and reactive oxygen metabolites (ROMs) and improve QoL in patients suffering from several chronic disorders. A prospective, observational study involved patients with gastrointestinal diseases who received a prescription of a cycle of sulphurous hydropinotherapy (S-HT). Age- and sex-matched control group was enrolled (No S-HT). Glycaemia and plasma concentration of ROMs were measured in all subjects. The impact of spa treatment on the QoL was assessed using the Short Form 36 Health Status Survey questionnaire (SF-36). All parameters were measured at baseline and at the end of a 2-week treatment. Between the groups, no differences were found in glycaemia and ROMs at baseline. In the S-HT group, a reduction in glycaemia and ROMs, both in respect to baseline (p = 0.005 and p = 0.031, respectively) and to control group, as shown by the delta value calculated, as the difference between the values at 2 weeks and baseline (p = 0.0009 and p = 0.0001, respectively). In the S-HT, delta ROMs was the best predictor of delta glycaemia with a direct linear correlation (beta = 0.559, 95% CI 0.471 to 0.647, p < 0.0001). In the S-HT, the SF-36 total score was improved both when compared with baseline (p = 0.002) and with No S-HT (p = 0.001). Sulphurous hydropinotherapy induces a decrease in glycaemia and ROM levels, also ameliorating the patients’ QoL. Therefore, it could be considered a useful complementary therapeutic approach.
Sulfane Sulfur Is a Strong Inducer of the Multiple Antibiotic Resistance Regulator MarR in Escherichia coli
Authors: Xu Huangwei , Xuan Guanhua , Liu Huaiwei , Xia Yongzhen , Xun Luying
Published: November 2021

Sulfane sulfur, including persulfide and polysulfide, is produced from the metabolism of sulfur-containing organic compounds or from sulfide oxidation. It is a normal cellular component, participating in signaling. In bacteria, it modifies gene regulators to activate the expression of genes involved in sulfur metabolism. However, to determine whether sulfane sulfur is a common signal in bacteria, additional evidence is required. The ubiquitous multiple antibiotic resistance regulator (MarR) family of regulators controls the expression of numerous genes, but the intrinsic inducers are often elusive. Recently, two MarR family members, Pseudomonas aeruginosa MexR and Staphylococcus aureus MgrA, have been reported to sense sulfane sulfur. Here, we report that Escherichia coli MarR, the prototypical member of the family, also senses sulfane sulfur to form one or two disulfide or trisulfide bonds between two dimers. Although the tetramer with two disulfide bonds does not bind to its target DNA, our results suggest that the tetramer with one disulfide bond does bind to its target DNA, with reduced affinity. An MarR-repressed mKate reporter is strongly induced by polysulfide in E. coli. Further investigation is needed to determine whether sulfane sulfur is a common signal of the family members, but three members sense cellular sulfane sulfur to turn on antibiotic resistance genes. The findings offer additional support for a general signaling role of sulfane sulfur in bacteria.
Antioxidants Characterization of the Fruit, Juice, and Pomace of Sweet Rowanberry (Sorbus aucuparia L.) Cultivated in Estonia
Authors: Sarv Viive , Venskutonis Petras Rimantas , Rätsep Reelika , Aluvee Alar , Kazernavičiūtė Rita , Bhat Rajeev
Published: November 2021

This study aimed to identify promising candidates of rowanberry cultivars for a wider cultivation and utilization. Antioxidant properties and phenolic content were evaluated for fruit, juice, and pomace samples of 16 different sweet rowanberry cultivars (cvs) and wild rowanberry (S. aucuparia L.), while the antioxidant potential was assessed using three different methods, based on the capacity to scavenge ABTS●+ and DPPH● and measure the oxygen radical absorbance capacity (ORAC). In general, the radical scavenging capacity was higher for hybrid cultivars, e.g., for cvs Likernaja, Burka, Granatnaja, and Rubinovaja in all assays. The highest value in the ABTS●+ assay was determined for the fruit sample Likernaja, and in DPPH● assay in the pomace sample of cv. Likernaja, at 527.55 and 1068.28 µM TE/g dw, respectively. The highest ORAC value was found in the fruit sample of Burka (456.53 µM TE/g dw). Among the Nevezhino rowans, the highest radical scavenging values of all fractions were determined in cv. Solnechnaja. Regarding the total phenolic content (TPC), higher values were obtained in the whole fruits than in separated fractions, juice, and pomace. The tested hybrids had higher TPC values, either in fruit and pomace or in juice extracts, than those in the other analyzed S. aucuparia L. cultivars. While the fruit and juice samples showed higher anthocyanin (ACY) values, the pomace samples had higher hydroxycinnamic acid (HCA) contents on average. The results revealed that the different fractions of selected rowanberry cultivars can be a promising source of antioxidants and polyphenols for further potential applications. It is envisaged that the results of this study will serve in valorizing sweet rowanberry cultivars as value-added functional ingredients for food and non-food applications.
Towards Climate Adaptation in Cities: Indicators of the Sustainable Climate-Adaptive Urban Fabric of Traditional Cities in West Asia
Authors: Alabsi Akram Ahmed Noman , Wu Yue , Koko Auwalu Faisal , Alshareem Khaled Mohammed , Hamed Roknizadeh
Published: November 2021

One of the most important criteria for a city’s sustainability is climate adaptation. Simultaneously, traditional cities in West Asia have achieved harmony between architecture and the environment, depending on the experience and culture of the local community, indicating a fundamental stage of adaptation to the environment and climate. This research attempted to study and examine the traditional urban fabric, in order to diagnose its significance and ability to solve contemporary problems, such as the lack of comfortable and suitable urban housing environments in hot–dry climate areas, which cover most of West Asia. We used two research methods to create an integrated vision to investigate the indicators and their effect on urban form. Four case studies in the urban fabric and housing communities of West Asia’s hot–dry research areas were chosen for the analytical study. Furthermore, inductive analysis was carried out to determine the most critical sustainability parameters in the particular urban fabric. The climate response parameters of the study cities were evaluated using Ecotect simulation tools, and the determinants of sustainability indicators were quantitatively evaluated to assess each indicator’s impact on the sustainability of the urban fabric. Our findings have implications for assessing the relevance of climate adaptation in the sustainability of cities and their effectiveness in combating the impacts of climate change.
Power-Based Concept for Current Injection by Inverter-Interfaced Distributed Generations during Transmission-Network Faults
Authors: Polajžer Boštjan , Grčar Bojan , Černelič Jernej , Ritonja Jožef
Published: November 2021

This paper analyzes the influence of inverter-interfaced distributed generations’ (IIDGs) response during transmission network faults. The simplest and safest solution is to switch IIDGs off during network faults without impacting the network voltages. A more elaborate and efficient concept, required by national grid codes, is based on controlling the IIDGs’ currents, involving positive- and negative-sequence voltage measured at the connection point. In this way the magnitude and phase of the injected currents can be adjusted, although the generated power will depend on the actual line voltages at the connection point. Therefore, an improved concept is proposed to adjust IIDGs’ fault current injection through the required active and reactive power, employing the same voltage characteristics. The proposed, i.e., power-based concept, is more definite than the current-based one, since the required power will always be generated. The discussed concepts for the fault current injection by IIDGs were tested in different 110-kV networks with loop and radial topologies, and for different short-circuit capabilities of the aggregated network supply. Based on extensive numerical calculations, the power-based concept during transmission networks faults generates more reactive power compared to the current-based concept. However, the voltage support by IIDGs during transmission networks faults, regardless of the concept being used, is influenced mainly by the short-circuit capability of the aggregated network supply. As regards distance protection operation, it is influenced additionally by the network topology, i.e., in radial network topology, the remote relay’s operation can be delayed due to a largely seen impedance.
Digital Eco-Design and Life Cycle Assessment—Key Elements in a Circular Economy: A Case Study of a Conventional Desk
Authors: Martínez-Cámara Eduardo , Santamaría Jacinto , Sanz-Adán Félix , Arancón David
Published: November 2021

In recent times, there has been an indisputable need to move towards a more sustainable economy, known as a circular economy, which is basically aimed at reducing the consumption of newly extracted raw materials to manufacture products, and thus, reduces waste generation by recycling products beyond their useful life to ultimately close the economic flow of the product. For the economy generated by products to close the circle, it is essential to tackle the problem at the source, that is, the process to achieve the desired product should be conducted by designing the product with environmental criteria (eco-design) and analysing its life cycle from the extraction process to the point when it ends its useful life (LCA). This article presents an ECO + LCA methodology that provides designers with an easy way of visualising the effect of their design decisions on the final environmental impact of the product. This methodology was tested on a case study of a conventional desk, with four alternative scenarios presented and an assessment of their final impact with a cradle-to-grave perspective. The final design obtained reduces the environmental impact by more than 30% and reduces costs by more than 11%.
Analysis and Optimization of the Robotic Mobile Fulfillment Systems Considering Congestion
Authors: Chi Cheng , Wang Yanyan , Wu Shasha , Zhang Jian
Published: November 2021

With the development of the social economy and the improvement of the consumption concept, a new business model combining offline and online has been promoted. The warehousing system is one of the important links of commodity production and circulation, which involves storage, sorting, and distribution. It has a significant impact on the operation cost and the efficiency of the whole logistics system. The progress of robot technology, the Internet of things, and artificial intelligence technology promotes the automation and intelligence of storage systems. The Robotic Mobile Fulfillment Systems (RMFS), which takes the automatic guided vehicles (AGVs) as the way of handling and picking, greatly improves the space utilization, operation efficiency, and flexibility of the system. This paper studies the RMFS with fixed shelves and establishes the performance evaluation model of the picking system considering the AGVs congestion by establishing the queuing network. The effectiveness of the model is verified by simulation, and the optimization of system parameter configuration is further discussed according to the experimental data.
Degradation of Oxytetracycline by Persulfate Activation Using a Magnetic Separable Iron Oxide Catalyst Derived from Hand-Warmer Waste
Authors: Lee Youn-Jun , Lee Chang-Gu , Park Seong-Jik , Jho Eun Hea
Published: November 2021

Oxytetracycline (OTC) is a tetracycline antibiotic that is widely used in the drug therapy and livestock industry and may threaten human health and ecosystems when released into the environment. In this study, a catalyst was prepared from hand-warmer waste using a simple magnetic separation method. The prepared hand-warmer waste catalyst (HWWC) was used as a persulfate (PS) activator for OTC removal. Characterization methods, such as X-ray diffraction and scanning electron microscopy–energy dispersive X-ray spectrometry, were used to investigate the crystal structure, surface morphology, and weight ratios of the elements in the HWWC. The degradation efficiency of OTC in the presence of the catalyst and PS was studied, and the radical generation mechanism of the catalyst was investigated. The removal ratio of OTC by PS activation was greater than 99% for a reaction time of 24 min at a pH of 6. The effects of the HWWC dosage, PS concentration, and solution pH on OTC degradation were also investigated. The reuse test revealed that HWWC can be reused for eight cycles with great stability. These results suggest that PS activation using hand-warmer waste can be an efficient strategy for the degradation of antibiotics.
UX in AR-Supported Industrial Human–Robot Collaborative Tasks: A Systematic Review
Authors: Khamaisi Riccardo Karim , Prati Elisa , Peruzzini Margherita , Raffaeli Roberto , Pellicciari Marcello
Published: November 2021

The fourth industrial revolution is promoting the Operator 4.0 paradigm, originating from a renovated attention towards human factors, growingly involved in the design of modern, human-centered processes. New technologies, such as augmented reality or collaborative robotics are thus increasingly studied and progressively applied to solve the modern operators’ needs. Human-centered design approaches can help to identify user’s needs and functional requirements, solving usability issues, or reducing cognitive or physical stress. The paper reviews the recent literature on augmented reality-supported collaborative robotics from a human-centered perspective. To this end, the study analyzed 21 papers selected after a quality assessment procedure and remarks the poor adoption of user-centered approaches and methodologies to drive the development of human-centered augmented reality applications to promote an efficient collaboration between humans and robots. To remedy this deficiency, the paper ultimately proposes a structured framework driven by User eXperience approaches to design augmented reality interfaces by encompassing previous research works. Future developments are discussed, stimulating fruitful reflections and a decisive standardization process.
Evaluation of Two Different Types of Mineral Trioxide Aggregate Cements as Direct Pulp Capping Agents in Human Teeth
Authors: Ballal Nidambur Vasudev , Rao Sheetal , Rao Nirmala , Urala Arun , Yoo Jun-Sang , Al-Haj Husain Nadin , Özcan Mutlu
Published: November 2021

Traumatic human dental injuries involving the pulp might necessitate direct capping procedures. This clinical study aimed to analyse the histological outcomes using two different direct capping materials. Twenty patients with bilateral premolars, scheduled for orthodontic extraction, were selected. The teeth were treated either using ProRoot MTA or RetroMTA. All patients were recalled after 30 and 60 days for teeth extraction. The histopathologically stained specimens were blindly evaluated using hard tissue bridge formation, inflammatory reaction and pulpal findings criteria. Data were evaluated statistically. Results: After 60 days, only the parameter for hard tissue bridge formation showed significant difference in the ProRoot MTA group (p = 0.010), while both direct capping materials performed similarly regarding inflammatory pulp reaction and pulpal findings. Although, during the first 30 days, RetroMTA presented better results in terms of continuity, morphology, hard tissue bridge localisation, and extension/general state of the inflammatory reaction, the continuity was better at 60 days when ProRoot MTA was applied. Treatment with RetroMTA healed the pulpal tissue faster compared with ProRoot MTA but it seemed to be rather a reparative process.
Key Criteria in the Choice of IoT Platforms in Spanish Companies
Authors: Infante-Moro Alfonso , Infante-Moro Juan C. , Gallardo-Pérez Julia , Martínez-López Francisco J.
Published: November 2021

The level of implementation of IoT technology in Spanish companies is not low for a novel technology, but there are concerns that hinder its level of implementation from being higher: initial costs, security, and privacy. These concerns could be solved with the choice of the appropriate IoT platform, but it must be taken into account that there are numerous criteria that participate in this decision that influence each other. This could give rise to criteria not being considered at first, even though they should be taken into account in this choice. Existing studies do not consider this. Thus, the objective of this study was to find out what are the key criteria to be taken into account by Spanish companies when choosing an IoT platform. The criteria were located through a literary review, and classified according to their level of importance in this decision through a causal study carried out with the methodology of fuzzy cognitive maps. The results achieved showed that there were key criteria beyond the criteria directly implied by the concerns, such as stability, scalability, flexibility, and interoperability, and these new criteria acquired the same or greater importance.
Formulation Development and Performance Characterization of Ecological Dust Suppressant for Road Surfaces in Cities
Authors: Wang Yuan , Du Cuifeng , Cui Mengmeng
Published: November 2021

In order to solve the problem of road dust pollution, an ecological dust suppressant for road surfaces has been developed using monomer, orthogonal, and optimization experiments and based on the dust raising mechanism. A humectant, hygroscopic agent, coagulant and surfactant and their concentration ranges have been determined through monomer experiment. The preliminary formula of the dust suppressant has been obtained through orthogonal experiment, with the water loss rate, moisture content rate, viscosity value, and surface tension value serving as experimental indexes. The optimal formula for the dust suppressor has been calculated through an optimization experiment, with the toxicity, moisture absorption and retention performance of plants, and the relative damage rate of plant seeds serving as experimental indexes. Based on the performance characterization of ecological road dust suppressant, the ecologically and environmentally friendly dust suppressant demonstrates fine moisture absorption and retention performance, good wind and rain erosion resistance, and no toxicity. The ecological road dust suppressant developed herein covers extensive raw material sources. It is ecologically and environmentally friendly, fit for most urban roads, and has a fine dust suppression effect. Meanwhile, it also can bring in good economic and social benefits, demonstrating its broad application prospects.
Bidirectional, Analog Current Source Benchmarked with Gray Molasses-Assisted Stray Magnetic Field Compensation
Authors: Dobosz Jakub , Bocheński Mateusz , Semczuk Mariusz
Published: November 2021

In ultracold-atom and ion experiments, flexible control of the direction and amplitude of a uniform magnetic field is necessary. It is achieved almost exclusively by controlling the current flowing through coils surrounding the experimental chamber. Here, we present the design and characterization of a modular, analog electronic circuit that enables three-dimensional control of a magnetic field via the amplitude and direction of a current flowing through three perpendicular pairs of coils. Each pair is controlled by one module, and we are able to continuously change the current flowing thorough the coils in the ±4 A range using analog waveforms such that smooth crossing through zero as the current’s direction changes is possible. With the electrical current stability at the 10−5 level, the designed circuit enables state-of-the-art ultracold experiments. As a benchmark, we use the circuit to compensate stray magnetic fields that hinder efficient sub-Doppler cooling of alkali atoms in gray molasses. We demonstrate how such compensation can be achieved without actually measuring the stray fields present, thus speeding up the process of optimization of various laser cooling stages.
Valorisation of Starch Wastewater by Anaerobic Fermentation
Authors: Drosg Bernhard , Neubauer Matthias , Marzynski Marceli , Meixner Katharina
Published: November 2021

Starch production is mainly focused on feedstocks such as corn, wheat and potato in the EU, whereas cassava, rice, and other feedstocks are utilised worldwide. In starch production, a high amount of wastewater is generated, which accumulates from different process steps such as washing, steeping, starch refining, saccharification and derivatisation. Valorisation of these wastewaters can help to improve the environmental impact as well as the economics of starch production. Anaerobic fermentation is a promising approach, and this review gives an overview of the different utilisation concepts outlined in the literature and the state of the technology. Among bioenergy recovery processes, biogas technology is widely applied at the industrial scale, whereas biohydrogen production is used at the research stage. Starch wastewater can also be used for the production of bulk chemicals such as acetone, ethanol, butanol or lactic acids by anaerobic microbes.
Comprehensive Analysis of CPA4 as a Poor Prognostic Biomarker Correlated with Immune Cells Infiltration in Bladder Cancer
Authors: Wei Chengcheng , Zhou Yuancheng , Xiong Qi , Xiong Ming , Hou Yaxin , Yang Xiong , Chen Zhaohui
Published: November 2021

Carboxypeptidase A4 (CPA4) has shown the potential to be a biomarker in the early diagnosis of certain cancers. However, no previous research has linked CPA4 to therapeutic or prognostic significance in bladder cancer. Using data from The Cancer Genome Atlas (TCGA) database, we set out to determine the full extent of the link between CPA4 and BLCA. We further analyzed the interacting proteins of CPA4 and infiltrated immune cells via the TIMER2, STRING, and GEPIA2 databases. The expression of CPA4 in tumor and normal tissues was compared using the TCGA + GETx database. The connection between CPA4 expression and clinicopathologic characteristics and overall survival (OS) was investigated using multivariate methods and Kaplan–Meier survival curves. The potential functions and pathways were investigated via gene set enrichment analysis. Furthermore, we analyze the associations between CPA4 expression and infiltrated immune cells with their respective gene marker sets using the ssGSEA, TIMER2, and GEPIA2 databases. Compared with matching normal tissues, human CPA4 was found to be substantially expressed. We confirmed that the overexpression of CPA4 is linked with shorter OS, DSF(Disease-specific survival), PFI(Progression-free interval), and increased diagnostic potential using Kaplan–Meier and ROC analysis. The expression of CPA4 is related to T-bet, IL12RB2, CTLA4, and LAG3, among which T-bet and IL12RB2 are Th1 marker genes while CTLA4 and LAG3 are related to T cell exhaustion, which may be used to guide the application of checkpoint blockade and the adoption of T cell transfer therapy.
Effect of Physical Exercise Program Based on Active Breaks on Physical Fitness and Vigilance Performance
Authors: González-Fernández Francisco Tomás , González-Víllora Sixto , Baena-Morales Salvador , Pastor-Vicedo Juan Carlos , Clemente Filipe Manuel , Badicu Georgian , Murawska-Ciałowicz Eugenia
Published: November 2021

The scientific literature has shown the beneficial effects of chronic Physical Exercise (PE) on a wide range of tasks that involve high-order functioning. For this reason, the present study aimed to investigate the effects of active breaks on physical fitness and vigilance performance in high school students through eight weeks of physical training. A total of 42 healthy students (age = 16.50 ± 0.59 years; height = 171.08 ± 8.07 cm; weight = 67.10 ± 13.76 kg) from one Andalusian high school (Spain) were assigned for convenience and matched into two groups, a Control Group (CG) and an Active-Break Group (ABG). The ABG performed two active breaks (based on strength and self-loading exercises) during the school day, first at 10:00 a.m. and second at 12:30 p.m. The participants were assessed before and after the training program using the Alpha-Fitness test battery and the Psychomotor Vigilance Task (PVT). Significant differences were observed in the post-training PVT results, compared with the pretraining PVT, showing ABG responding faster than CG. Thus, the presents study demonstrated that eight weeks of physical training affects vigilance performance (compared to CG) and improves the efficiency of vigilance in high school students, contributing to enhancement of quality of education.
Tissue Regeneration and Physiological Functional Recovery in Dental and Craniofacial Fields
Authors: Ouchi Takehito , Nakagawa Taneaki
Published: November 2021

Dental and oral tissues maintain homeostasis through potential reparative or regenerative processes [...]
Editorial on the Special Issue: “Pancreatic Islets of Langerhans: Not Only Beta-Cells”
Authors: Malaguarnera Roberta , Piro Salvatore
Published: November 2021

This year marks the centenary of the discovery of insulin [...]
Computational Insights into the Binding Mechanism of OxyS sRNA with Chaperone Protein Hfq
Authors: Li Mengxin , Cong Yalong , Qi Yifei , Zhang John Z. H.
Published: November 2021

Under the oxidative stress condition, the small RNA (sRNA) OxyS that acts as essential post-transcriptional regulators of gene expression is produced and plays a regulatory function with the assistance of the RNA chaperone Hfq protein. Interestingly, experimental studies found that the N48A mutation of Hfq protein could enhance the binding affinity with OxyS while resulting in the defection of gene regulation. However, how the Hfq protein interacts with sRNA OxyS and the origin of the stronger affinity of N48A mutation are both unclear. In this paper, molecular dynamics (MD) simulations were performed on the complex structure of Hfq and OxyS to explore their binding mechanism. The molecular mechanics generalized born surface area (MM/GBSA) and interaction entropy (IE) method were combined to calculate the binding free energy between Hfq and OxyS sRNA, and the computational result was correlated with the experimental result. Per-residue decomposition of the binding free energy revealed that the enhanced binding ability of the N48A mutation mainly came from the increased van der Waals interactions (vdW). This research explored the binding mechanism between Oxys and chaperone protein Hfq and revealed the origin of the strong binding affinity of N48A mutation. The results provided important insights into the mechanism of gene expression regulation affected by protein mutations.
Drought Resistance Loci in Recombinant Lines of Iranian Oryza sativa L. in Germination Stage
Authors: Noryan Morteza , Hervan Islam Majidi , Sabouri Hossein , Kojouri Faroukh Darvish , Mastinu Andrea
Published: November 2021

In order to locate control genes related to Oryza sativa L. traits at the germination stage under normal conditions and at drought stress levels (−4.5 and −9.0 bar), we evaluated 120 F8 generation offspring from the cross between two cultivars Neda × Ahlemitarum in a factorial experiment in a completely randomized block design with three replications in 2013 in the botanical laboratory of Gonbad Kavous University. A linkage map was prepared using 90 Simple Sequence Repeats (SSR) markers and 28 Inter Simple Sequence Repeats (ISSR), and 6 iPBS and 9 IRAP markers (265 polymorphic alleles). The results of the analysis of variance showed that all of the evaluated traits had a significant difference at the probability level of 1%. Hence, it can be noted that the desired genetic diversity can be found between genotypes. The results of the stepwise regression analysis for the germination percentage as a dependent variable and other traits as independent variables in the studied treatments showed that under normal conditions, there was variable coleoptile length, but under drought stress of −4.5 and −9.0 bar, the variable plumule dry weight entered the model. In this study, the markers included in RM1-RM490 and ISSR2-3-RM133 of chromosomes 1 and 6 of Oryza sativa were identified as the main regulators of traits associated with Oryza sativa drought resistance. In particular, they present the quantitative trait loci (QTL) that control the first stages of germination of Oryza sativa in water stress conditions.
Communicating Risk: Developing an “Efficiency Index” for Dementia Screening Tests
Authors: Larner Andrew J.
Published: November 2021

Diagnostic and screening tests may have risks such as misdiagnosis, as well as the potential benefits of correct diagnosis. Effective communication of this risk to both clinicians and patients can be problematic. The purpose of this study was to develop a metric called the “efficiency index” (EI), defined as the ratio of test accuracy and inaccuracy, to evaluate screening tests for dementia. This measure was compared with a previously described “likelihood to be diagnosed or misdiagnosed” (LDM), also based on “numbers needed” metrics. Datasets from prospective pragmatic test accuracy studies examining four brief cognitive screening instruments (Mini-Mental State Examination; Montreal Cognitive Assessment; Mini-Addenbrooke’s Cognitive Examination (MACE); and Free-Cog) were analysed to calculate values for EI and LDM, and to examine their variation with test cut-off for MACE and dementia prevalence. EI values were also calculated using a modification of McGee’s heuristic for the simplification of likelihood ratios to estimate percentage change in diagnostic probability. The findings indicate that EI is easier to calculate than LDM and, unlike LDM, may be classified either qualitatively or quantitatively in a manner similar to likelihood ratios. EI shows the utility or inutility of diagnostic and screening tests, illustrating the inevitable trade-off between diagnosis and misdiagnosis. It may be a useful metric to communicate risk in a way that is easily intelligible for both clinicians and patients.
Working towards Culturally Responsive Trauma-Informed Care in the Refugee Resettlement Process: Qualitative Inquiry with Refugee-Serving Professionals in the United States
Authors: Im Hyojin , Swan Laura E. T.
Published: November 2021

Trauma-informed care (TIC) approaches have gained popularity in various contexts of human services over the past decades. However, relatively little has been explored about how it is applicable and built into services for refugee populations in resettlement programs. This study explores the current status of the application of TIC in refugee-serving agencies and identifies perceived and experienced challenges and opportunities for culturally responsive TIC in the United States. As designed as part of the evaluation of state-wide refugee health promotion programs, this study conducted individual interviews with 78 refugee service providers from five resettlement sites. Despite the burgeoning interest and attempt to embrace TIC, our findings show that there is clear inconsistency and inexperience in TIC adaptation in resettlement programs. This study highlights that TIC that is culturally responsive and relevant to refugee trauma and acculturation experiences is a vital way to address the chasms between refugee-specific programs and mainstream services including mental health care systems. This study also discusses community resources and opportunities to bridge the deep divide and substantial gaps between mental health services and refugee resettlement services and to address comprehensive needs around mental health and wellness in the refugee community.
A Comprehensive Evaluation Method for Air-Conditioning System Plants Based on Building Performance Simulation and Experiment Information
Authors: Lyu Yan , Pan Yiqun , Yuan Xiaolei , Zhu Mingya , Huang Zhizhong , Kosonen Risto
Published: November 2021

During the design stage of an HVAC (heating, ventilation, and air conditioning) system in a construction project, designers must decide on the most workable design scheme for the plant room in the building based on the evaluation of multiple aspects related to system performance that need to be considered, such as energy efficiency, economic effectiveness, etc. To solve this problem, this paper proposes a comprehensive evaluation method for the plant rooms of centralized air-conditioning systems in commercial buildings. This new method consists of two analyses used in tandem: Building Performance Simulation (BPS) models and a collection of real HVAC design cases (the carried-out design solutions). The BPS models and a knowledge of the reduction approach based on Rough Set (RS) theory are used to generate data and weight factors for the indices of energy efficiency; and the real design cases are employed with a heuristic algorithm to extract the compiled empirical information for other evaluation items of the centralized HVAC system. In addition, this paper also demonstrates an application in an actual case of a building construction project. By comparing the expert decision-making process and the evaluation results, it is found that they are basically consistent, which verifies the reasonability of the comprehensive evaluation method.
Scenario-Based Comprehensive Assessment for Community Resilience Adapted to Fire Following an Earthquake, Implementing the Analytic Network Process and Preference Ranking Organization Method for Enriched Evaluation II Techniques
Authors: He Zheng , Chen Huihua , Yan Hongyan , Yin Yang , Qiu Qi , Wang Tingpeng
Published: November 2021

Natural hazards bring significant influences on and socioeconomic loss to cities and communities. Historic events show that fire following earthquake (FFE) is the most influential uncertain disturbance on the urban infrastructure system. Under the FFE scenario, the concept of resilience is widely implemented to make up the shortcomings derived from the traditional disaster management methodology. Resilient cities and communities are required to improve the systemic performance in responding to the FFE. To fulfill these goals, measuring community resilience is an essential work for municipal policy makers. Therefore, this study conducted a comprehensive assessment on community resilience adapted to the FFE scenario. The systematic literature review (SLR) was employed to identify the indicators, and the analytic network process (ANP) technique was implemented to determine their weights. 20 indicators were extracted, and 4 communities that encountered FFE in China were selected for the empirical analysis. Thereafter, the preference ranking organization method for enriched evaluation (PROMETHEE) II technique was selected through using the multicriteria decision analysis (MCDA) methods selection framework to fulfill the comprehensive assessment. The results were discussed and demonstrated with graphical analysis for interactive aid (GAIA) technique. The findings revealed that the G Community won the highest score and had the strongest performance. However, H Community had the lowest score and the weakest performance. The proposed comprehensive methods could benefit the decision-makers and the policy executors achieving the community resilience adapted to the FFE scenario by improving the effective indicators.
Constructing g-C3N4/Cd1−xZnxS-Based Heterostructures for Efficient Hydrogen Production under Visible Light
Authors: Zhurenok Angelina V. , Markovskaya Dina V. , Gerasimov Evgeny Y. , Vokhmintsev Alexander S. , Weinstein Ilya A. , Prosvirin Igor P. , Cherepanova Svetlana V. , Bukhtiyarov Andrey V. , Kozlova Ekaterina A.
Published: November 2021

Two types of photocatalysts, 1%Pt/Cd1−xZnxS/g-C3N4 (x = 0.2–0.3) and Cd1−xZnxS/1%Pt/g-C3N4 (x = 0.2–0.3), were synthesized by varying the deposition order of platinum, and a solid solution of cadmium and zinc sulfides onto the surface of g-C3N4. The characterization of photocatalysts showed that, for 1%Pt/Cd1−xZnxS/g-C3N4, small platinum particles were deposited onto a solid solution of cadmium and zinc sulfides; in the case of Cd1−xZnxS/1%Pt/g-C3N4, enlarged platinum clusters were located on the surface of graphitic carbon nitride. Based on the structure of the photocatalysts, we assumed that, in the first case, type II heterojunctions and, in the latter case, S-scheme heterojunctions were realized. The activity of the synthesized samples was tested in hydrogen evolution from triethanolamine (TEOA) basic solution under visible light (λ = 450 nm). A remarkable increase in hydrogen evolution rate compared to single-phase platinized 1%Pt/Cd1−xZnxS photocatalysts was observed only in the case of ternary photocatalysts with platinum located on the g-C3N4 surface, Cd1−xZnxS/1%Pt/g-C3N4. Thus, we proved using kinetic experiments and characterization techniques that, for composite photocatalysts based on Cd1−xZnxS and g-C3N4, the formation of the S-scheme mechanism is more favorable than that for type II heterojunction. The highest activity, 2.5 mmol H2 g−1 h−1, with an apparent quantum efficiency equal to 6.0% at a wavelength of 450 nm was achieved by sample 20% Cd0.8Zn0.2S/1% Pt/g-C3N4.
Identification and Functional Analysis of lncRNAs Responsive to Hypoxia in Eospalax fontanierii
Authors: Hao Zhiqiang , Han Mingfang , Guo Juanjuan , Li Guanglin , He Jianping , Li Jingang
Published: November 2021

Subterranean rodents could maintain their normal activities in hypoxic environments underground. Eospalax fontanierii, as one kind of subterranean rodent found in China can survive very low oxygen concentration in labs. It has been demonstrated that long non-coding RNAs (lncRNAs) have important roles in gene expression regulations at different levels and some lncRNAs were found as hypoxia-regulated lncRNAs in cancers. We predicted thousands of lncRNAs in the liver and heart tissues by analyzing RNA-Seq data in Eospalax fontanierii. Those lncRNAs often have shorter lengths, lower expression levels, and lower GC contents than mRNAs. Majors of lncRNAs have expression peaks in hypoxia conditions. We found 1128 DE-lncRNAs (differential expressed lncRNAs) responding to hypoxia. To search the miRNA regulation network for lncRNAs, we predicted 471 and 92 DE-lncRNAs acting as potential miRNA target and target mimics, respectively. We also predicted the functions of DE-lncRNAs based on the co-expression networks of lncRNA-mRNA. The DE-lncRNAs participated in the functions of biological regulation, signaling, development, oxoacid metabolic process, lipid metabolic/biosynthetic process, and catalytic activity. As the first study of lncRNAs in Eospalax fontanierii, our results show that lncRNAs are popular in transcriptome widely and can participate in multiple biological processes in hypoxia responses.
Safe Return to Work for Domestic Workers in the Time of COVID-19
Authors: Bardhan Rupkatha , Byrd Traci , Boyd Julie
Published: November 2021

Domestic workers including housecleaners, nannies, and caregivers are facing a challenging time in the era of COVID-19 exposure. Many domestic workers have lost their jobs worldwide. As businesses and organizations have started to reopen in full capacity, domestic workers are unsure of their future and whether they will be rehired by their employers. They have less protections from labor laws unlike other occupations and usually their employers/agencies do not provide training on safe practices for working in a home setting. There are gaps in understanding safety and health issues associated with precarious work for domestic workers. This review article has searched the literature on safe strategies for domestic workers to eliminate exposure and provides helpful suggestions for domestic workers to safely return to work. Employers or house owners can have a proper reopening plan when considering hiring or rehiring domestic workers. Domestics working in a home environment should use best practices to protect themselves and others from infectious diseases. Having open communication between employers and their domestic workers can go a long way. Implementing and following an effective working plan for both employers and their domestic workers will provide a path towards minimization of hazard and control of infectious diseases like COVID-19.