bluuebunny/crossref_metadata_2025 huggingface dataset:
Sol–Gel-Derived Glass-Ceramic Photorefractive Films for Photonic Structures
Authors: Lukowiak Anna , Zur Lidia , Tran Thi , Meneghetti Marcello , Berneschi Simone , Nunzi Conti Gualtiero , Pelli Stefano , Trono Cosimo , Bhaktha B.N. , Zonta Daniele , Taccheo Stefano , Righini Giancarlo , Ferrari Maurizio
Published: February 2017

Glass photonics are widespread, from everyday objects around us to high-tech specialized devices. Among different technologies, sol–gel synthesis allows for nanoscale materials engineering by exploiting its unique structures, such as transparent glass-ceramics, to tailor optical and electromagnetic properties and to boost photon-management yield. Here, we briefly discuss the state of the technology and show that the choice of the sol–gel as a synthesis method brings the advantage of process versatility regarding materials composition and ease of implementation. In this context, we present tin-dioxide–silica (SnO2–SiO2) glass-ceramic waveguides activated by europium ions (Eu3+). The focus is on the photorefractive properties of this system because its photoluminescence properties have already been discussed in the papers presented in the bibliography. The main findings include the high photosensitivity of sol–gel 25SnO2:75SiO2 glass-ceramic waveguides; the ultraviolet (UV)-induced refractive index change (Δn ~ −1.6 × 10−3), the easy fabrication process, and the low propagation losses (0.5 ± 0.2 dB/cm), that make this glass-ceramic an interesting photonic material for smart optical applications.
The Effects of Salinity and pH on Fertilization, Early Development, and Hatching in the Crown-of-Thorns Seastar
Authors: Allen Jonathan , Schrage Kharis , Foo Shawna , Watson Sue-Ann , Byrne Maria
Published: February 2017

Understanding the influence of environmental factors on the development and dispersal of crown-of-thorns seastars is critical to predicting when and where outbreaks of these coral-eating seastars will occur. Outbreaks of crown-of-thorns seastars are hypothesized to be driven by terrestrial runoff events that increase nutrients and the phytoplankton food for the larvae. In addition to increasing larval food supply, terrestrial runoff may also reduce salinity in the waters where seastars develop. We investigated the effects of reduced salinity on the fertilization and early development of seastars. We also tested the interactive effects of reduced salinity and reduced pH on the hatching of crown-of-thorns seastars. Overall, we found that reduced salinity has strong negative effects on fertilization and early development, as shown in other echinoderm species. We also found that reduced salinity delays hatching, but that reduced pH, in isolation or in combination with lower salinity, had no detectable effects on this developmental milestone. Models that assess the positive effects of terrestrial runoff on the development of crown-of-thorns seastars should also consider the strong negative effects of lower salinity on early development including lower levels of fertilization, increased frequency of abnormal development, and delayed time to hatching.
A Risk-Free Protection Index Model for Portfolio Selection with Entropy Constraint under an Uncertainty Framework
Authors: Gao Jianwei , Liu Huicheng
Published: February 2017

This paper aims to develop a risk-free protection index model for portfolio selection based on the uncertain theory. First, the returns of risk assets are assumed as uncertain variables and subject to reputable experts’ evaluations. Second, under this assumption, combining with the risk-free interest rate we define a risk-free protection index (RFPI), which can measure the protection degree when the loss of risk assets happens. Third, note that the proportion entropy serves as a complementary means to reduce the risk by the preset diversification requirement. We put forward a risk-free protection index model with an entropy constraint under an uncertainty framework by applying the RFPI, Huang’s risk index model (RIM), and mean-variance-entropy model (MVEM). Furthermore, to solve our portfolio model, an algorithm is given to estimate the uncertain expected return and standard deviation of different risk assets by applying the Delphi method. Finally, an example is provided to show that the risk-free protection index model performs better than the traditional MVEM and RIM.
The More You Know, the More You Can Grow: An Information Theoretic Approach to Growth in the Information Age
Authors: Hilbert Martin
Published: February 2017

In our information age, information alone has become a driver of social growth. Information is the fuel of “big data” companies, and the decision-making compass of policy makers. Can we quantify how much information leads to how much social growth potential? Information theory is used to show that information (in bits) is effectively a quantifiable ingredient of growth. The article presents a single equation that allows both to describe hands-off natural selection of evolving populations and to optimize population fitness in uncertain environments through intervention. The setup analyzes the communication channel between the growing population and its uncertain environment. The role of information in population growth can be thought of as the optimization of information flow over this (more or less) noisy channel. Optimized growth implies that the population absorbs all communicated environmental structure during evolutionary updating (measured by their mutual information). This is achieved by endogenously adjusting the population structure to the exogenous environmental pattern (through bet-hedging/portfolio management). The setup can be applied to decompose the growth of any discrete population in stationary, stochastic environments (economic, cultural, or biological). Two empirical examples from the information economy reveal inherent trade-offs among the involved information quantities during growth optimization.
Multilingual Researchers Internationalizing Monolingual English-Only Education through Post-Monolingual Research Methodologies
Authors: Singh Michael
Published: February 2017

The argument advanced in this Special Issue of Education Sciences favors democratizing knowledge production and dissemination across the humanities and social sciences through the mainstreaming of multilingual researchers capabilities for theorizing using their full linguistic repertoire. An important contribution of the papers in this Special Issue is the promise that post-monolingual research methodology holds for collaborative projects among multilingual and monolingual researchers that tap into intercultural divergences across languages. Together these papers give warrant to multilingual researchers, including Higher Degree Researchers develop their capabilities for theorizing using their full linguistic repertoire, an educational innovation that could be of immense benefit to scholars working predominantly monolingual universities. Through their thought provoking papers presented in this Special Issue, these researchers invites those working in the education sciences to seriously consider the potential benefits of multiplying the intellectual resources used for theorizing that is possible through activating, mobilizing and deploying researchers’ multilingual resources in knowledge production and dissemination.
Religious Education towards Justice: What Kind of Justice Is to Be Taught in a Christian Context?
Authors: Bobbert Monika
Published: February 2017

Education is a human right. It prepares human beings for life, helps to develop individual abilities and opens up social opportunities—e.g., earning one’s own living. Religion interprets our human existence in connection to a transcendental dimension. Religion can also influence moral values and behavior. The Christian religion established a basis for social life, and thus deals with religious and moral justice. As the Christian faith is understood as the identity of the qualities of love of God, of your neighbor and even of your enemy, it has to look for justice in the world. Modern Christian ethics does unfold interpersonal and global justice for all people and tries to give good reasons for moral claims. Religious education in a Christian context has to answer the question of what kind of justice is to be taught and by what means justice, as a goal of education, can be reached within such a setting. This article will unfold, from an ethical point of view, what kind of knowledge and competence teachers must have and what kind of goals can be followed with regard to their pupils or students. The results of this reflection imply certain pedagogical methods and means and exclude others—although it is not possible to go more deeply into a pedagogical discussion.
Exposure Assessment Methods in Studies on Waste Management and Health Effects: An Overview
Authors: Spinazzè Andrea , Borghi Francesca , Rovelli Sabrina , Cavallo Domenico
Published: February 2017

Concerns and uncertainties persist about potential environmental and health effects associated with exposure to emissions from widely adopted waste management facilities: despite a limited amount of evidence having been found for some exposure-effect associations, most of the available studies were characterized by limitations related to poor exposure assessment, which could introduce biases and weaknesses in the interpretation of results. This communication provides a brief overview of the exposure assessment methods used in studies on waste management and health effects: problems, key issues, priorities and challenges are briefly presented and discussed. The main conclusions refer to the need of newly developed and harmonized exposure assessment strategies and techniques, which represent an essential step in the study of waste-disposal facilities’ health impacts.
Can the Palatability of Healthy, Satiety-Promoting Foods Increase with Repeated Exposure during Weight Loss?
Authors: Anguah Katherene , Lovejoy Jennifer , Craig Bruce , Gehrke Malinda , Palmer Philip , Eichelsdoerfer Petra , McCrory Megan
Published: February 2017

Repeated exposure to sugary, fatty, and salty foods often enhances their appeal. However, it is unknown if exposure influences learned palatability of foods typically promoted as part of a healthy diet. We tested whether the palatability of pulse containing foods provided during a weight loss intervention which were particularly high in fiber and low in energy density would increase with repeated exposure. At weeks 0, 3, and 6, participants (n = 42; body mass index (BMI) 31.2 ± 4.3 kg/m2) were given a test battery of 28 foods, approximately half which had been provided as part of the intervention, while the remaining half were not foods provided as part of the intervention. In addition, about half of each of the foods (provided as part or not provided as part of the intervention) contained pulses. Participants rated the taste, appearance, odor, and texture pleasantness of each food, and an overall flavor pleasantness score was calculated as the mean of these four scores. Linear mixed model analyses showed an exposure type by week interaction effect for taste, texture and overall flavor pleasantness indicating statistically significant increases in ratings of provided foods in taste and texture from weeks 0 to 3 and 0 to 6, and overall flavor from weeks 0 to 6. Repeated exposure to these foods, whether they contained pulses or not, resulted in a ~4% increase in pleasantness ratings. The long-term clinical relevance of this small increase requires further study.
Swap Equilibria under Link and Vertex Destruction
Authors: Kliemann Lasse , Shirazi Sheykhdarabadi Elmira , Srivastav Anand
Published: February 2017

We initiate the study of the destruction or adversary model (Kliemann 2010) using the swap equilibrium (SE) stability concept (Alon et al., 2010). The destruction model is a network formation game incorporating the robustness of a network under a more or less targeted attack. In addition to bringing in the SE concept, we extend the model from an attack on the edges to an attack on the vertices of the network. We prove structural results and linear upper bounds or super-linear lower bounds on the social cost of SE under different attack scenarios. For the case that the vertex to be destroyed is chosen uniformly at random from the set of max-sep vertices (i.e., where each causes a maximum number of separated player pairs), we show that there is no tree SE with only one max-sep vertex. We conjecture that there is no tree SE at all. On the other hand, we show that for the uniform measure, all SE are trees (unless two-connected). This opens a new research direction asking where the transition from “no cycle” to “at least one cycle” occurs when gradually concentrating the measure on the max-sep vertices.
Association of Sleep Duration, Sleep Quality and Shift-Work Schedule in Relation to Hypertension Prevalence in Chinese Adult Males: A Cross-Sectional Survey
Authors: Lu Kai , Chen Jia , Wang Li , Wang Changying , Ding Rongjing , Wu Shouling , Hu Dayi
Published: February 2017

Background: Previous studies indicated that measurement of sleep only by duration and quality may be biased. This study aimed to investigate the interactive association of self-reported sleep duration, quality and shift-work schedule with hypertension prevalence in Chinese adult males. Methods: A total of 4519 Chinese adult males (≥18 years) were enrolled into the cross-sectional survey. Sleep attributes were measured from the responses to the standard Pittsburgh Sleep Quality Index and relevant questions in a structured questionnaire survey. The association of sleep duration, quality and shift-work schedule with hypertension prevalence was analyzed using multivariate logistic regression, considering the interaction between them or not. Results: Taking the potential interaction of the three aspects of sleep into consideration, only short sleep duration combined with poor sleep quality was found to be related to hypertension prevalence in Chinese adult males (odds ratio (OR): 1.74, 95% confidence interval (CI): 1.31–2.31), which could be modified by occasional and frequent shift-work schedule (OR: 1.43, 95% CI: 1.05–1.95; OR: 1.97, 95% CI: 1.40–2.79). Conclusions: Short sleep duration was not associated with the prevalence of hypertension in Chinese adult males unless poor sleep quality exists, which could be further modified by shift-work schedule. Assessment of sleep by measuring sleep duration only was not sufficient when exploring the association of sleep with hypertension.
Comparison of Influenza Epidemiological and Virological Characteristics between Outpatients and Inpatients in Zhejiang Province, China, March 2011–June 2015
Authors: Cheng Wei , Yu Zhao , Liu Shelan , Zhang Xueying , Wang Xiaoxiao , Cai Jian , Ling Feng , Chen Enfu
Published: February 2017

Given the rapid rate of global spread and consequently healthcare costs related to influenza, surveillance plays an important role in monitoring the emerging pandemics in China. However, the characteristics of influenza in Southeast of China haven’t been fully studied. Our study use the surveillance data collected from 16 sentinel hospitals across Zhejiang Province during March 2011 through June 2015, including the demographic information and respiratory specimens from influenza-like illness (ILI) patients and severe acute respiratory illness (SARI) patients. As analysis results, most SARI and ILI patients were in the age group of 0–4 years old (62.38% of ILI and 71.54% of SARI). The respiratory specimens have statistically significantly higher positive rate for influenza among ILI patients than that among SARI patients (p < 0.001). The comparison between ILI patients and SARI patients shows no statistically significantly difference in detecting influenza virus type and influenza A virus subtype. The SARI and ILI patients were found to be positively correlated for overall positive rate (r = 0.63, p < 0.001), the weekly percentage of A(H1N1)pdm09 (r = 0.51, p < 0.001), influenza B virus (r = 0.17, p = 0.013), and A/H3N2 (r = 0.43, p < 0.001) among all the positive numbers. Our study demonstrated that the activities of influenza virus, including its subtypes, had a similar temporal pattern between ILI and SARI cases.
Regions Set in Stone—Delimiting and Categorizing Regions in Europe by Settlement Patterns Derived from EO-Data
Authors: Taubenböck Hannes , Ferstl Joachim , Dech Stefan
Published: February 2017

The spatial patterns of landscapes are complex. Highly dense urban centers are not just mirrowed in a dichotomic sense by rural environments; landscapes are a spatially variable continuum. In this logic, nation-states (or any political or administrative unit) spatially integrate different types and physical appearances of land cover. Understanding regions in the sense that similar physical characteristics may construct alternative (natural) spatial entities which may sub-divide or cross-over adminstrative boundaries allows us to overcome common map projections. However, which indicators and which regional logics define and delimit regions is conceptually vague. With this paper we aim to add an empirical study to identify regional phenomena in Europe. To do so, we take advantage of a new data set from remote sensing, the Global Urban Footprint. It features European-wide consistent spatial information on settlement patterns. We use density and distribution of settlements as indicators for delimiting regions by similar characteristics. Our methodological approach classifies urban nodes (by settlement density and size), spans an unbounded soft space by the classification of spatial connectivity between nodes (by continuous settlement) and maps territorial entities (by density around nodes); the approach is following a space of place logic. From a geographic perspective we identify uneven development across Europe. The corridor streching from England via the Benelux areas via Germany, Switzerland, France to Northern Italy is mapped as the European backbone; however, new focal areas such as, e.g., towards eastern Europe are also detected. Applying a plausibility check reveals that the proxy settlement pattern corresponds well with regional conceptions presented in other studies.
The Temporal Pattern, Flux, and Function of Autophagy in Spinal Cord Injury
Authors: Zhou Kailiang , Sansur Charles , Xu Huazi , Jia Xiaofeng
Published: February 2017

Previous studies have indicated that autophagy plays a critical role in spinal cord injury (SCI), including traumatic spinal cord injury (TSCI) and ischemia-reperfusion spinal cord injury (IRSCI). However, while the understanding of mechanisms underlying autophagy in SCI has progressed, there remain several controversial points: (1) temporal pattern results of autophagic activation after SCI are not consistent across studies; (2) effect of accumulation of autophagosomes due to the blockade or enhancement of autophagic flux is uncertain; (3) overall effect of enhanced autophagy remains undefined, with both beneficial and detrimental outcomes reported in SCI literature. In this review, the temporal pattern of autophagic activation, autophagic flux, autophagic cell death, relationship between autophagy and apoptosis, and pharmacological intervention of autophagy in TSCI (contusion injury, compression injury and hemisection injury) and IRSCI are discussed. Types of SCI and severity appear to contribute to differences in outcomes regarding temporal pattern, flux, and function of autophagy. With future development of specific strategies on autophagy intervention, autophagy may play an important role in improving functional recovery in patients with SCI.
Next-Generation Sequencing Approach in Methylation Analysis of HNF1B and GATA4 Genes: Searching for Biomarkers in Ovarian Cancer
Authors: Bubancova Ivana , Kovarikova Helena , Laco Jan , Ruszova Ema , Dvorak Ondrej , Palicka Vladimir , Chmelarova Marcela
Published: February 2017

DNA methylation is well-known to be associated with ovarian cancer (OC) and has great potential to serve as a biomarker in monitoring response to therapy and for disease screening. The purpose of this study was to investigate methylation of HNF1B and GATA4 and correlate detected methylation with clinicopathological characteristic of OC patients. The study group consisted of 64 patients with OC and 35 control patients. To determine the most important sites of HNF1B and GATA4, we used next-generation sequencing. For further confirmation of detected methylation of selected regions, we used high-resolution melting analysis and methylation-specific real-time polymerase chain reaction (PCR). Selected regions of HNF1B and GATA4 were completely methylation free in all control samples, whereas methylation-positive pattern was observed in 32.8% (HNF1B) and 45.3% (GATA4) of OC samples. Evaluating both genes together, we were able to detect methylation in 65.6% of OC patients. We observed a statistically significant difference in HNF1B methylation between samples with different stages of OC. We also detected subtype specific methylation in GATA4 and a decrease of methylation in late stages of OC. The combination of unmethylated HNF1B and methylated GATA4 was associated with longer overall survival. In our study, we employed innovative approach of methylation analysis of HNF1B and GATA4 to search for possible epigenetic biomarkers. We confirmed the significance of the HNF1B and GATA4 hypermethylation with emphasis on the need of selecting the most relevant sites for analysis. We suggest selected CpGs to be further examined as a potential positive prognostic factor.
Candida Species Biofilms’ Antifungal Resistance
Authors: Silva Sónia , Rodrigues Célia , Araújo Daniela , Rodrigues Maria , Henriques Mariana
Published: February 2017

Candida infections (candidiasis) are the most prevalent opportunistic fungal infection on humans and, as such, a major public health problem. In recent decades, candidiasis has been associated to Candida species other than Candida albicans. Moreover, biofilms have been considered the most prevalent growth form of Candida cells and a strong causative agent of the intensification of antifungal resistance. As yet, no specific resistance factor has been identified as the sole responsible for the increased recalcitrance to antifungal agents exhibited by biofilms. Instead, biofilm antifungal resistance is a complex multifactorial phenomenon, which still remains to be fully elucidated and understood. The different mechanisms, which may be responsible for the intrinsic resistance of Candida species biofilms, include the high density of cells within the biofilm, the growth and nutrient limitation, the effects of the biofilm matrix, the presence of persister cells, the antifungal resistance gene expression and the increase of sterols on the membrane of biofilm cells. Thus, this review intends to provide information on the recent advances about Candida species biofilm antifungal resistance and its implication on intensification of the candidiasis.
Compressive Strength Enhancement of Vertically Aligned Carbon Nanotube Forests by Constraint of Graphene Sheets
Authors: Su Chih-Chung , Chen Ting-Xu , Chang Shuo-Hung
Published: February 2017

We fabricated a 3D sandwich hybrid material composed of graphene and vertically aligned carbon nanotube forests (VACNTs) using chemical vapor deposition. The graphene was first synthesized on Cu foil. Then it was transferred to a substrate which had a pre-deposited catalyst Fe film and a buffer film of Al2O3 for the growth of VACNTs. The VACNTs were grown underneath the graphene and lifted up the graphene. The graphene, with its edges anchored on the Al2O3, provided a constrained boundary condition for the VACNTs and hence affected the growth height and mechanical strength of the VACNTs. We prepared three groups of samples: VACNTs without graphene, VACNTs with graphene transferred once (1-Gr/VACNTs), and VACNTs with graphene transferred twice (2-Gr/VACNTs). A nano-indentation system was used to measure the reduced compressive modulus (Er) and hardness (H). The Er and H of Gr/VACNTs increased with the number of transfers of the anchored graphene. The 2-Gr/VACNTs had the largest Er and H, 23.8 MPa and 912 KPa, which are 6.6 times and 5.2 times those of VACNTs without the anchored graphene, respectively. In this work, we have demonstrated a simple method to increase the mechanical properties and suppress the height of VACNTs with the anchored graphene and number of transfers.
Effect of Energy Input on Microstructure and Mechanical Properties of Titanium Aluminide Alloy Fabricated by the Additive Manufacturing Process of Electron Beam Melting
Authors: Mohammad Ashfaq , Alahmari Abdulrahman , Mohammed Muneer , Renganayagalu Ravi , Moiduddin Khaja
Published: February 2017

Titanium aluminides qualify adequately for advanced aero-engine applications in place of conventional nickel based superalloys. The combination of high temperature properties and lower density gives an edge to the titanium aluminide alloys. Nevertheless, challenges remain on how to process these essentially intermetallic alloys in to an actual product. Electron Beam Melting (EBM), an Additive Manufacturing Method, can build complex shaped solid parts from a given feedstock powder, thus overcoming the shortcomings of the conventional processing techniques such as machining and forging. The amount of energy supplied by the electron beam has considerable influence on the final build quality in the EBM process. Energy input is decided by the beam voltage, beam scan speed, beam current, and track offset distance. In the current work, beam current and track offset were varied to reflect three levels of energy input. Microstructural and mechanical properties were evaluated for these samples. The microstructure gradually coarsened from top to bottom along the build direction. Whereas higher energy favored lath microstructure, lower energy tended toward equiaxed grains. Computed tomography analysis revealed a greater amount of porosity in low energy samples. In addition, the lack of bonding defects led to premature failure in the tension test of low energy samples. Increase in energy to a medium level largely cancelled out the porosity, thereby increasing the strength. However, this trend did not continue with the high energy samples. Electron microscopy and X-ray diffraction investigations were carried out to understand this non-linear behavior of the strength in the three samples. Overall, the results of this work suggest that the input energy should be considered primarily whenever any new alloy system has to be processed through the EBM route.
Global Modulus-Based Synchronous Multisplitting Multi-Parameters TOR Methods for Linear Complementarity Problems
Authors: Zhang Li-Tao , Gu Tong-Xiang
Published: February 2017

In 2013, Bai and Zhang constructed modulus-based synchronous multisplitting methods for linear complementarity problems and analyzed the corresponding convergence. In 2014, Zhang and Li studied the weaker convergence results based on linear complementarity problems. In 2008, Zhang et al. presented global relaxed non-stationary multisplitting multi-parameter method by introducing some parameters. In this paper, we extend Bai and Zhang’s algorithms and analyze global modulus-based synchronous multisplitting multi-parameters TOR (two parameters overrelaxation) methods. Moverover, the convergence of the corresponding algorithm in this paper are given when the system matrix is an H + -matrix.
An Initial Condition Optimization Approach for Improving the Prediction Precision of a GM(1,1) Model
Authors: Madhi Mahdi , Mohamed Norizan
Published: February 2017

Grey model GM(1,1) has attained excellent prediction accuracy with restricted data and has been broadly utilized in a range of areas. However, the GM(1,1) forecasting model sometimes yields large forecasting errors which directlyaffect the simulation and prediction precision directly. Therefore, the improvement of the GM(1,1) model is an essential issue, and the current study aims to enhance the prediction precision of the GM(1,1) model. Specifically, in order to improve the prediction precision of GM(1,1) model, it is necessary to consider improving the initial condition in the response function of the model. Consequently, the purpose of this paper is to put forward a new method to enhance the performance of the GM(1,1) model by optimizing its initial condition. The minimum sum of squared error was used to optimize the new initial condition of the model. The numerical outcomes show that the improved GM(1,1) model provides considerably better performance than traditional grey model GM(1,1) . The result demonstrates that the improved grey model GM(1,1) achieves the objective of minimizing the forecast errors.
Vochysia rufa Stem Bark Extract Protects Endothelial Cells against High Glucose Damage
Authors: Moura de Gouveia Neire , Ramos Sonia , Martín María , Espindola Foued , Goya Luis , Palomino Olga
Published: February 2017

Background: Increased oxidative stress by persistent hyperglycemia is a widely accepted factor in vascular damage responsible for type 2 diabetes complications. The plant Vochysia rufa (Vr) has been used in folk medicine in Brazil for the treatment of diabetes. Thus; the protective effect of a Vr stem bark extract against a challenge by a high glucose concentration on EA.hy926 (EA) endothelial cells is evaluated. Methods: Vegetal material is extracted with distilled water by maceration and evaporated until dryness under vacuum. Then; it is isolated by capillary electrophoresis–tandem mass spectrometry. Cell viability is evaluated on EA cells treated with 0.5–100 µg/mL of the Vr extract for 24 h. The extract is diluted at concentrations of 5, 10 and 25 µg/mL and maintained for 24 h along with 30 mM of glucose to evaluate its protective effect on reduced glutathione (GSH); glutathione peroxidase (GPx) and reductase (GR) and protein carbonyl groups. Results: V. rufa stem bark is composed mainly of sugars; such as inositol; galactose; glucose; mannose; sacarose; arabinose and ribose. Treatment with Vr up to 100 µg/mL for 24 h did not affect cell viability. Treatment of EA cells with 30 mM of glucose for 24 h significantly increased the cell damage. EA cells treated with 30 mM of glucose showed a decrease of GSH concentration and increased Radical Oxygen Species (ROS) and activity of antioxidant enzymes and protein carbonyl levels; compared to control. Co-treatment of EA with 30 mM glucose plus 1–10 μg/mL Vr significantly reduced cell damage while 5–25 μg/mL Vr evoked a significant protection against the glucose insult; recovering ROS; GSH; antioxidant enzymes and carbonyls to baseline levels. Conclusion: V. rufa extract protects endothelial cells against oxidative damage by modulating ROS; GSH concentration; antioxidant enzyme activity and protein carbonyl levels.
ShenLingLan Influences the Attachment and Migration of Ovarian Cancer Cells Potentially through the GSK3 Pathway
Authors: Owen Sioned , Ruge Fiona , Gao Yunong , Yang Ying , Hou Jianqing , Chen Jian , Feng Yi , Zhang Huiming , Gao Yong , Wang Hongtao , Wei Cong , Wu Yiling , Jiang Wen
Published: February 2017

Background: Ovarian cancer presents a major clinical challenge in the UK. Glycogen synthase kinase-3 (GSK-3) has been linked to cancer. This study tested the impact of ShenLingLan (SLDM) on ovarian cancer cell behaviour and its links to GSK-3. Methods: Fresh ovarian tumours (n = 52) were collected and processed. Histopathologcial and clinical information were collected and analysed against GSK-3 transcript levels using quantitative PCR (qPCR). Immortalised ovarian cancer cells’ protein alterations in response to SLDM were identified using a Kinexus™ protein kinase array. The effects of SLDM and a combination of SLDM and TWS119 on ovarian cancer cells ability to attach and migrate were evaluated using electrical cell-substrate impedance sensing (ECIS). Results: Transcript expression of GSK-3β was significantly increased in ovarian tumours which were poorly differentiated, patients with recurrence and in patients who had died from ovarian cancer. Treating SKOV-3 ovarian cells with SLDM reduced GSK-3 expression and GSK-3α (Y279). Treatment with SLDM reduced ovarian cancer cells ability to attach and migrate, which was further reduced in the presence of TWS119. Conclusions: This study identified a potential mechanism by which SLDM may exert anti-metastatic effects. Further work is needed to investigate the in vivo effects SLDM has on ovarian tumours.
Sino-Austrian High-Tech Acupuncture Network—Annual Report 2016
Authors: Litscher Gerhard
Published: February 2017

The Sino-Austrian High-Tech Acupuncture Research Network was founded in 2005 and has been growing ever since. The network comprises many partners from China and is highly involved in research and education activities. This report introduces the network’s activities in the year 2016.
Effect of Process and Post-Process Conditions on the Mechanical Properties of an A357 Alloy Produced via Laser Powder Bed Fusion
Authors: Aversa Alberta , Lorusso Massimo , Trevisan Francesco , Ambrosio Elisa , Calignano Flaviana , Manfredi Diego , Biamino Sara , Fino Paolo , Lombardi Mariangela , Pavese Matteo
Published: February 2017

A357 samples were realized by laser powder bed fusion (LPBF) on building platforms heated up to different temperatures. The effect of the preheating temperature and of the post processing heat treatment on the microstructure and the mechanical properties of the samples was studied. It was demonstrated that building platform heating can act as an in situ ageing heat treatment following the fast cooling that arises during laser scanning. A 17% higher ultimate tensile strength was achieved by the selection of the optimum building platform temperature. Moreover, the possibility to further increase the mechanical properties by means of a direct ageing heat treatment was investigated.
Synthesis, Characterization and Antibacterial Studies of N-(Benzothiazol-2-yl)-4-chlorobenzenesulphonamide and Its Neodymium(III) and Thallium(III) Complexes
Authors: Obasi Lawrence , Oruma Uchechukwu , Al-Swaidan Ibrahim , Ramasami Ponnadurai , Ezeorah Chigozie , Ochonogor Alfred
Published: February 2017

N-(Benzothiazol-2-yl)-4-chlorobenzenesulphonamide (NBTCS) was synthesized by condensation reaction of 4-chlorobenzenesulphonyl chloride and 2-aminobenzothiazole in acetone under reflux. Neodymium(III) and thallium(III) complexes of the ligand were also synthesized. Both ligand and metal complexes were characterized using UV-Vis, IR, 1H- and 13C-NMR spectroscopies, elemental analysis and molar conductance measurement. IR studies revealed that the ligand is tridentate and coordinates to the metal ions through nitrogen and oxygen atoms of the sulphonamide group and nitrogen atom attached to benzothiazole ring. The neodymium(III) complex displays a coordination number of eight while thallium(III) complex displays a coordination number of six. The ligand and its complexes were screened in vitro for their antibacterial activities against Escherichia coli strains (E. coli 6 and E. coli 13), Proteus species, Staphylococcus aureus and Pseudomonas aeruginosa using the agar well diffusion technique. The synthesized compounds were found to be more active against the microorganisms screened relative to ciprofloxacin, gentamicin and co-trimoxazole.
Immobilized Trienzymatic System with Enhanced Stabilization for the Biotransformation of Lactose
Authors: Torres Pedro , Batista-Viera Francisco
Published: February 2017

The use of ketohexose isomerases is a powerful tool in lactose whey processing, but these enzymes can be very sensitive and expensive. Development of immobilized/stabilized biocatalysts could be a further option to improve the process. In this work, β-galactosidase from Bacillus circulans, l-arabinose (d-galactose) isomerase from Enterococcus faecium, and d-xylose (d-glucose) isomerase from Streptomyces rubiginosus were immobilized individually onto Eupergit C and Eupergit C 250 L. Immobilized activity yields were over 90% in all cases. With the purpose of increasing thermostability of derivatives, two post-immobilization treatments were performed: alkaline incubation to favor the formation of additional covalent linkages, and blocking of excess oxirane groups by reacting with glycine. The greatest thermostability was achieved when alkaline incubation was carried out for 24 h, producing l-arabinose isomerase-Eupergit C derivatives with a half-life of 379 h and d-xylose isomerase-Eupergit C derivatives with a half-life of 554 h at 50 °C. Preliminary assays using immobilized and stabilized biocatalysts sequentially to biotransform lactose at pH 7.0 and 50 °C demonstrated improved performances as compared with soluble enzymes. Further improvements in ketohexose productivities were achieved when the three single-immobilizates were incubated simultaneously with lactose in a mono-reactor system.
Preparation of Photoirradiation Molecular Imprinting Polymer for Selective Separation of Branched Cyclodextrins
Authors: Fan Haoran , Wang Jinpeng , Meng Qingran , Xu Xueming , Fan Tianming , Jin Zhengyu
Published: February 2017

In the present study, photoirradiation molecularly imprinted polymer (MIP) with azobenzene was used as a functional monomer for the selective separation of the branched cyclodextrins. The functional monomer 4-methacryloyloxy azobenzene (MAA) and the molecular template 6-O-α-d-maltosyl-β-cyclodextrin (G2-β-CD) were implemented for the molecular imprinting. The core-shell structure of photoirradiation MIP was visualized by the transmission electron microscopy (TEM). With Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), we identified that G2-β-CD was imprinted into the polymer and removed from the MIP. The binding association constant (Ka) and the maximum number of the binding site (Nmax) were 1.72 × 104 M−1 and 7.93 μmol·g−1 MIP, respectively. With alternate irradiation at 365 and 440 nm light, the prepared MIP reversibly released and rebound to the G2-β-CD, which resulted in the nearly zero amount of G2-β-CD in the solution. The HPLC results indicated that the purity of G2-β-CD could reach 90.8% after going through MIP. The main finding of our study was that the photoirradiation of MIP was an easy and effective method for the selective separation of the branched cyclodextrins.
Metabolic Profiling and Identification of Shikonins in Root Periderm of Two Invasive Echium spp. Weeds in Australia
Authors: Skoneczny Dominik , Weston Paul , Zhu Xiaocheng , Gurr Geoff , Callaway Ragan , Barrow Russel , Weston Leslie
Published: February 2017

Metabolic profiling can be successfully implemented to analyse a living system’s response to environmental conditions by providing critical information on an organism’s physiological state at a particular point in time and allowing for both quantitative and qualitative assessment of a specific subset(s) of key metabolites. Shikonins are highly reactive chemicals that affect various cell signalling pathways and possess antifungal, antibacterial and allelopathic activity. Based on previous bioassay results, bioactive shikonins, are likely to play important roles in the regulation of rhizosphere interactions with neighbouring plants, microbes and herbivores. An effective platform allowing for rapid identification and accurate profiling of numerous structurally similar, difficult-to-separate bioactive isohexenylnaphthazarins (shikonins) was developed using UHPLC Q-TOF MS. Root periderm tissues of the invasive Australian weeds Echium plantagineum and its congener E. vulgare were extracted overnight in ethanol for shikonin profiling. Shikonin production was evaluated at seedling, rosette and flowering stages. Five populations of each species were compared for qualitative and quantitative differences in shikonin formation. Each species showed little populational variation in qualitative shikonin production; however, shikonin was considerably low in one population of E. plantagineum from Western New South Wales. Seedlings of all populations produced the bioactive metabolite acetylshikonin and production was upregulated over time. Mature plants of both species produced significantly higher total levels of shikonins and isovalerylshikonin > dimethylacrylshikonin > shikonin > acetylshikonin in mature E. plantagineum. Although qualitative metabolic profiles in both Echium spp. were nearly identical, shikonin abundance in mature plant periderm was approximately 2.5 times higher in perennial E. vulgare extracts in comparison to those of the annual E. plantagineum. These findings contribute to our understanding of the biosynthesis of shikonins in roots of two related invasive plants and their expression in relation to plant phenological stage.
One-Pot Conversion of Epoxidized Soybean Oil (ESO) into Soy-Based Polyurethanes by MoCl2O2 Catalysis
Authors: Pantone Vincenzo , Annese Cosimo , Fusco Caterina , Fini Paola , Nacci Angelo , Russo Antonella , D’Accolti Lucia
Published: February 2017

An innovative and eco-friendly one-pot synthesis of bio-based polyurethanes is proposed via the epoxy-ring opening of epoxidized soybean oil (ESO) with methanol, followed by the reaction of methoxy bio-polyols intermediates with 2,6-tolyl-diisocyanate (TDI). Both synthetic steps, methanolysis and polyurethane linkage formation, are promoted by a unique catalyst, molybdenum(VI) dichloride dioxide (MoCl2O2), which makes this procedure an efficient, cost-effective, and environmentally safer method amenable to industrial scale-up.
A Photocatalytic Rotating Disc Reactor with TiO2 Nanowire Arrays Deposited for Industrial Wastewater Treatment
Authors: Li Fang , Szeto Wai , Huang Haibao , Li Jiantao , Leung Dennis
Published: February 2017

A photocatalytic rotating disc reactor (PRD-reactor) with TiO2 nanowire arrays deposited on a thin Ti plate is fabricated and tested for industrial wastewater treatment. Results indicate that the PRD-reactor shows excellent decolorization capability when tested with methyl orange (>97.5%). Advanced oxidation processes (AOP), including photocatalytic oxidation and photolytic reaction, occurred during the processing. Efficiency of the AOP increases with reduction in light absorption pathlength, which enhanced the photocatalytic reaction, as well as by increasing oxygen exposure of the wastewater thin film due to the rotating disc design. It is found that, with a small dosage of hydrogen peroxide, the mineralization efficiency of industrial biodegraded wastewater can be enhanced, with a superior mineralization of >75% total organic carbon (TOC) removal. This is due to the fact that the TiO2 photocatalysis and hydrogen peroxide processes generate powerful oxidants (hydroxyl radicals) that can strongly improve photocatalytic oxidation efficiency. Application of this industrial wastewater treatment system is benefited from the TiO2 nanowire arrays, which can be fabricated by a mild solvothermal method at 80 °C and under atmospheric pressure. Similar morphologies and microstructures are found for the TiO2 nanowire arrays deposited on a large metal Ti disc, which makes the wastewater treatment process more practical and economical.
Synthesis of Reusable Silica Nanosphere-Supported Pt(IV) Complex for Formation of Disulfide Bonds in Peptides
Authors: Hou Xiaonan , Zhao Xiaowei , Zhang Yamei , Han Aiying , Huo Shuying , Shen Shigang
Published: February 2017

Some peptide-based drugs, including oxytocin, vasopressin, ziconotide, pramlintide, nesiritide, and octreotide, contain one intramolecular disulfide bond. A novel and reusable monodispersed silica nanosphere-supported Pt(IV) complex (SiO2@TPEA@Pt(IV)); TPEA: N-[3-(trimethoxysilyl)propyl]ethylenediamine) was synthesized via a four-step procedure and was used for the formation of intramolecular disulfide bonds in peptides. Transmission electron microscopy (TEM) and chemical mapping results for the Pt(II) intermediates and for SiO2@TPEA@Pt(IV) show that the silica nanospheres possess a monodisperse spherical structure and contain uniformly-distributed Si, O, C, N, Cl, and Pt. The valence state of Pt on the silica nanospheres was characterized by X-ray photoelectron spectroscopy (XPS). The Pt(IV) loaded on SiO2@TPEA@Pt(IV) was 0.15 mmol/g, as determined by UV-VIS spectrometry. The formation of intramolecular disulfides in six dithiol-containing peptides of variable lengths by the use of SiO2@TPEA@Pt(IV) was investigated, and the relative oxidation yields were determined by high-performance liquid chromatography (HPLC). In addition, peptide 1 (Ac-CPFC-NH2) was utilized to study the reusability of SiO2@TPEA@Pt(IV). No significant decrease in the relative oxidation yield was observed after ten reaction cycles. Moreover, the structure of SiO2@TPEA@Pt(IV) after being used for ten cycles was determined to be similar to its initial one, demonstrating the cycling stability of the complex.
Recent Advances and Applications of Molecular Docking to G Protein-Coupled Receptors
Authors: Bartuzi Damian , Kaczor Agnieszka , Targowska-Duda Katarzyna , Matosiuk Dariusz
Published: February 2017

The growing number of studies on G protein-coupled receptors (GPCRs) family are a source of noticeable improvement in our understanding of the functioning of these proteins. GPCRs are responsible for a vast part of signaling in vertebrates and, as such, invariably remain in the spotlight of medicinal chemistry. A deeper insight into the underlying mechanisms of interesting phenomena observed in GPCRs, such as biased signaling or allosteric modulation, can be gained with experimental and computational studies. The latter play an important role in this process, since they allow for observations on scales inaccessible for most other methods. One of the key steps in such studies is proper computational reconstruction of actual ligand-receptor or protein-protein interactions, a process called molecular docking. A number of improvements and innovative applications of this method were documented recently. In this review, we focus particularly on innovations in docking to GPCRs.
Design, Synthesis and Evaluation of Naphthalimide Derivatives as Potential Anticancer Agents for Hepatocellular Carcinoma
Authors: Ge Chaochao , Chang Liping , Zhao Ying , Chang Congcong , Xu Xiaojuan , He Haoying , Wang Yuxia , Dai Fujun , Xie Songqiang , Wang Chaojie
Published: February 2017

Two kinds of naphthalimide derivatives were synthesized and evaluated for in vitro their anti-hepatocellular carcinoma properties. Compound 3a with a fused thiazole fragment to naphthalimide skeleton inhibited cell migration of SMMC-7721 and HepG2, and further in vivo trials with two animal models confirmed that compound 3a moderately inhibited primary H22 tumor growth (52.6%) and potently interrupted lung metastasis (75.7%) without obvious systemic toxicity at the therapeutic dose. Mechanistic research revealed that compound 3a inhibited cancerous liver cell growth mostly by inducing G2/M phase arrest. Western blotting experiments corroborated that 3a could up-regulate the cell cycle related protein expression of cyclin B1, CDK1 and p21, and inhibit cell migration by elevating the E-cadherin and attenuating integrin α6 expression. Our study showed that compound 3a is a valuable lead compound worthy of further investigation.
Antimicrobial Polymers in the Nano-World
Authors: Álvarez-Paino Marta , Muñoz-Bonilla Alexandra , Fernández-García Marta
Published: February 2017

Infections are one of the main concerns of our era due to antibiotic-resistant infections and the increasing costs in the health-care sector. Within this context, antimicrobial polymers present a great alternative to combat these problems since their mechanisms of action differ from those of antibiotics. Therefore, the microorganisms’ resistance to these polymeric materials is avoided. Antimicrobial polymers are not only applied in the health-care sector, they are also used in many other areas. This review presents different strategies that combine nanoscience and nanotechnology in the polymer world to combat contaminations from bacteria, fungi or algae. It focuses on the most relevant areas of application of these materials, viz. health, food, agriculture, and textiles.