bluuebunny/crossref_metadata_2025 huggingface dataset:
Immunomodulatory effect of turmeric, amla, and pepper mixture against sepsis-induced acute lung injury in rats
Authors: Nagaraju M. , Kalahasti Krishna Kalyan , Reddy K. Prathap , Addi Utkarsh Reddy , Satyavani M. , Reddy G. Bhanuprakash , R S. Sreenivasa
Published: May 2022

Acute lung injury (ALI), is a severe inflammatory lung disease. We tested the prophylactic effect of a functional food comprising three different anti-inflammatory plant products: turmeric, amla, and black pepper (TAB) against lipopolysaccharide (LPS)-induced ALI in rats. Two-month-old male Wistar rats were randomly divided into three groups: control (C), LPS (5mg/kg), and LPS with TAB (TAB). After 6 hours of LPS injection, the rats were sacrificed by cervical decapitation to collect the lung tissue. Results showed that TAB partially ameliorated LPS-induced circulating inflammatory cytokines (TNFα and IL6) and significantly prevented lung histopathological changes. TAB also suppressed LPS-activated ER stress markers (GRP78, pIRE1, and CHOP) and apoptotic proteins (caspase-3 and -12) in the lung. The anti-inflammatory effects of the TAB support its potential use at least as an adjuvant to mitigate ALI. Importantly, the ingredients of TAB are in use for centuries as part of their diet with limited or no toxic effects identified.
Pyroglutamic Acidosis – An Underrecognized Entity Associated with Acetaminophen Use
Authors: Ng Wincy Wing-Sze , Tong Hok-Fung , Ng Wai-Yan , Yeung Joshua Ka-Ho , Young Joyce Kit-Yu , Woo Raymond Kam-Wing , Wong Maureen Mo-Lin
Published: May 2022

Pyroglutamic acidosis (PGA) is an underrecognized entity characterised by raised anion gap metabolic acidosis (RAGMA) and urinary hyper-excretion of pyroglutamic acid. It is frequently associated with chronic acetaminophen (APAP) ingestion. We report the case of a 73-year-old man with invasive pulmonary aspergillosis treated with voriconazole, and APAP for analgesia with a cumulative dose of 160g over 40 days. PGA was suspected as he developed severe RAGMA and which common causes were excluded. Diagnosis was confirmed by urinary organic acid analysis showing significant hyper-excretion of pyroglutamic acid. APAP was discontinued and N-acetylcysteine (NAC) was administered. His RAGMA rapidly resolved following treatment.
A model of chemotherapy-induced tumor dormancy: doxorubicin retention and degradation defines resumed growth in vivo
Authors: Zaleskis Gintaras , Garberytė Sima , Pavliukevičienė Božena , Characiejus Dainius , Žilionytė Karolina , Kraśko Jan Aleksander , Karabanovas Vitalijus , Matusevičienė Nijolė , Kučinskaitė Vakarė Barbora , Pasukoniene Vita
Published: May 2022

Background.
Cancer recurrence after adjuvant chemotherapy with long periods of remission is common. After cessation of the therapy, the dormant cells may repopulate, but the signals that control the tumor to exit dormancy are not completely understood. We hypothesized that tissue bound cytotoxic drug indwelling in a body for years might somehow contribute to dormancy or recurrence of a tumor. The purpose of this study was to design a model demonstrating that viable cells implanted in mice can repopulate or be suppressed, depending on the cytotoxic preload conditions.
Methods
. A two-step dormancy/recurrence (TSDR) murine model was designed, which mimics the extrusion of taken up drug from tumor cells. The viable cells preloaded with drug were implanted into mice. The survival rates of these mice were then used as criteria to demonstrate the relationship between resumed growth and drug cellular efflux/viability. The drug internalization patterns following their exposure to doxorubicin (dox) or degraded dox (dox-dgr) were investigated by exploring flow cytometry, spectral analysis, high performance liquid chromatography and confocal microscopy. Antiproliferative and myelotoxic capacity was evaluated by hematological nadir induced by the iv injected drug.
Results.
The viable SL2 lymphoma cells exposed to 10 µg/ml of dox for 30 min and injected into syngeneic DBA/2 mice were made unable to recure. Exposure of cells to lower dox concentrations (0.01–1.0 µg/ml) resulted in tumor recurrence, similar to that which was observed during implantation of an untreated tumor. Dox-dgr kept at 37°C for 365 days lost its tumoricidal and antiproliferative capacity and displayed a loss of selectivity of nuclear fluorescence.
Conclusions.
Our TSDR model is a rapid convenient tool to study in vivo behavior of cells preloaded with cytotoxic drug. This approach focuses on mechanisms of tumor cells exiting dormancy, in relation to cytotoxic drug efflux. Multiple modifications of our TSDR model are possible, including nude mice models. As an example, we used one-year body temperature exposed dox to demonstrate its inability to retain sufficient cytotoxic capacity.
Determinants of consumers’ purchase intention to buy smartphones online
Authors: Julfikar Ali Mohammed , Mobarak Karim Md. , Ishaque Hitoishi Benazir , M. Atif Wafik H. , M. Anwarul Islam K.
Published: May 2022

In this technological era, online business is expanding due to increased internet conveniences. Smartphone users are drastically growing in Bangladesh, where it plays a vital role as a strong catalyst for economic development. On the other hand, the success of the online business is associated with creating online purchase tendency by determining influencing factors of consumers’ intention. The ultimate purpose of this paper is to identify the influential variables of consumers’ purchase intention to buy smartphones on online platforms. To fulfill the aim, a structured questionnaire has been elaborated based on the literature review to find this study’s objective. Cronbach’s alpha value was used to assess the inner surface of this study. The non-probability convenience sampling method and a 5% significance level are used to accept the hypotheses. Altogether, 350 survey questionnaires were distributed among the online-based social media customers, and finally, 288 responses were received genuinely, where the response rate was 82%. Henceforward, the sample size of the study is n = 288. SPSS version 26.0 has been applied to scrutinize hypothesis tests in this study. This paper also offers implications for online business people and several impactful guidelines for future research studies.
Simulation analysis of UF membrane filtration process based on magnetic field characteristics
Authors: Guo Xingfei , Wang Yu , Wang Lianjin , Chao Shulin , Li Pengfei
Published: May 2022

Membrane fouling will affect the performance of the membrane, resulting in the increase of filtration cost and reduced membrane life. Magnetization technology has the advantages of being green, pollution-free and energy saving, and it has broad potential applications in water treatment. In this study, COMSOL Multiphysics realizes the coupling of magnetic field and flow field by adding the magnetic field force formula in the fluid module. The influence of magnetic field on fluid flow and inorganic pollutant distribution in an ultrafiltration (UF) membrane device were investigated. The simulation results show that the magnetic field improves the water flow velocity in the device to a certain extent, reduces the volume fraction of pollutant particles on the membrane surface, and then reduces the possibility of forming a thicker filter cake layer, which is beneficial to slow down membrane fouling and thus slow down the reduction rate of membrane flux. In addition, the simulation results were verified by the membrane specific flux experiment, and consistent conclusions were obtained.
Study on treatment of acid red G with bio-carbon compound immobilized white rot fungi
Authors: Wu Huifang , Xu Xinyu , Qin Yu , Jiang Yu , Lin Zhen
Published: May 2022

Corn straw biochar was used as a carrier to immobilize white rot fungi and the removal performance of immobilized pellets for acid red G (ARG) dye was studied. The results showed that the removal rate of ARG could reach 96.17% under the best preparation conditions of immobilized pellets (3% sodium alginate concentration, 0.7% corn straw biochar, 5% white rot fungus mycelium suspension, 4% CaCl2, and 36 h immobilization time). The orthogonal test results showed that the best combination was the immobilized pellets dosage of 200/100 mL, pH of 4.5, rotation speed of 150 r/min, and initial concentration of 20 mg/L dye at 30 °C. The degradation pathway of ARG by immobilized microspheres was studied by ultraviolet-visible spectrometer, Fourier transform infrared spectroscopy, and liquid chromatography-mass spectrometry. The results showed that ARG was degraded into aniline and 5-(acetamino)-4-hydroxy-3-amino-2,7-naphthalene disulfonic acid. Aniline was further deaminated to form benzene, and benzene was ring opened to form other organic compounds; 5-(acetylamino)-4-hydroxy-3-amino-2,7-naphthalene disulfonic acid was dehydroxylated to form 5-(acetylamino)-3-amino-2,7-naphthalene disulfonic acid. This study shows that the prepared biochar immobilized pellets can be used as an efficient water treatment material to remove ARG dye from wastewater.
The Bajo Guadalhorce coastal aquifers (Málaga, Spain): five decades of human induced evolution
Authors: Nieto López José Manuel , Andreo Navarro Bartolomé , Linares Girela Luis , Carrasco Cantos Francisco
Published: May 2022

This work makes a synthesis of the hydrogeological knowledge acquired from different works from the decade of 1970 in the Bajo Guadalhorce detrital, coastal aquifers. Hydrodynamic, hydrochemical and geophysical studies were done in the frame of the aforementioned works to determine the hydrogeological properties of these aquifers. Besides, the drilling of research piezometers was carried out in the framework of some of these studies. This information allowed to study the spatial and temporal behavior of groundwater table and some chemical components of the groundwater of these aquifers, as well as to estimate some parameters as hydraulic conductivity or transmissivity. Overexploitation of aquifers was also studied, because marine intrusion situations were detected over the years. However, some studies carried out in the decade of 2010 have remarked a noticeable difference in the hydrodynamic and hydrochemical state of aquifers, due to intense changes in land use during the end of the 20th century. These changes have also influenced over some groundwater dependent ecosystems, as the Guadalhorce Delta Wetlands, located in the Guadalhorce River mouth. So, this article is an example of the remarkable influence that human activities can have on groundwater due to irrigated agriculture, a rising tourism and the building of big hydraulic infrastructures, which demonstrate the necessity of an adequate management of Mediterranean coastal aquifers.
Bringing the Spotlight to Tau and TDP-43 in Frontotemporal Dementia:
A Review of Promising Chemical Compounds
Authors: Villalobos-Nova Karla , Monroy-Moya Sebastián , Maulen-Peñaloza Joaquín , Pinto Gabriela C.M. , Cornejo Alberto
Published: May 2022

There is a wide variety of neurodegenerative diseases, among which frontotemporal
dementia stands out. These are the second most frequent cause of dementia in the
world and demand the search for an effective treatment. This disease is linked to the abnormal
behavior of proteins, which group together to form insoluble aggregates. It has
been shown that the tau protein and TDP-43 are the main proteins involved in these
pathologies. This article details 11 compounds already used in different neuropathologies,
which may serve as potential drugs against these proteins. The mechanism of how
most of these molecules inhibited the tau and TDP-43 aggregation process was highlighted.
Importantly, Curcumin, Proanthocyanidin B2, Oleocanthal, Oleuropein Aglycone,
Thionine, and Resveratrol had been reported as direct inhibitors of tau. While 4-aminoquinoline,
Dimethoxycurcumin, and Auranofin directly inhibited TDP-43. Epigallocatechin-
3- gallate and Methylene Blue were described as tau and TDP-43 inhibitors. In this
review, it is proposed that future research could elucidate the detailed inhibition mechanisms
of these compounds to obtain relevant data to advance in treatments search for these
coexisting proteins in frontotemporal dementia.
Exploring the Mechanism of Yi-Jing Decoction in Treating Polycystic
Ovary Syndrome by Using Network Pharmacology
Authors: Lin Yue , Xiang Liu , Li Xianhai , Tang Qiang , Meng Fanbo , Chen Wei
Published: May 2022

Background:

Yi-Jing decoction (YJD), a traditional Chinese medicine prescription,
has been reported to be effective in the treatment of polycystic ovary syndrome
(PCOS). However, the underlying mechanisms of YJD in treating PCOS are still unclear.

Objective:

In the present work, the effective ingredients of YJD and their treatment
mechanisms on PCOS were systematically analyzed.

Methods:

The effective ingredients of YJD and targets of PCOS were selected from public
databases. The network pharmacology method was used to analyze the ingredients, potential
targets, and pathways of YJD for the treatment of PCOS.

Results:

One hundred and three active ingredients were identified from YJD, of which 82
were hit by 65 targets associated with PCOS. By constructing the disease-common targetcompound
network, five ingredients (quercetin, arachidonate, beta-sitosterol, betacarotene,
and cholesterol) were selected out as the key ingredients of YJD, which can interact
with the 10 hub genes (VEGFA, AKT1, TP53, ALB, TNF, PIK3CA, IGF1, INS,
IL1B, PTEN) against PCOS. These genes are mainly involved in prostate cancer, steroid
hormone biosynthesis, and EGFR tyrosine kinase inhibitor resistance pathways. In addition,
the results of molecular docking showed that the ingredients of YJD have a good
binding affinity with the hub genes.

Conclusion:

These results demonstrate that the treatment of PCOS by YJD is through
regulating the levels of androgen and insulin and improving the inflammatory microenvironment.
Biosynthetic Pathways for Rebeccamycin Family of Natural Products
Authors: Varala Ravi , Alam M. Mujahid , Kurmarayuni Chandra Mohan , Bollikolla Hari Babu , Dubasi Narsimhaswamy
Published: May 2022

The isolation of Rebeccamycin, a family of indolocarbazole alkaloids from natural sources,
and the biosynthesis of this class of compounds are briefly reviewed. Rebeccamycin and its analogues
have been extensively studied by medicinal chemists, over the last four decades, due to its wide range
of biological activities, predominantly with neuroprotective and antitumor properties.
Recombinant Human Lactoferrin Augments Epirubicin Chemotherapy in
Solid Ehrlich Carcinoma Bearing Mice
Authors: El-Ashmawy Nahla E. , Khedr Eman G. , El-Kady Amira Y. , Al-Ashmawy Ghada M.
Published: May 2022

Background:

Lactoferrin (LF) is a member of the transferrin family, which is known for
its immunomodulatory properties. LF has been widely used as an anticancer medication in various
cancers including breast cancer.

Aims:

The current study aimed to examine the molecular mechanisms underlying the therapeutic
potential of recombinant human lactoferrin (rhLF), either alone or combined with epirubicin (EPI),
in mice bearing solid Ehrlich carcinoma (SEC).

Methods:

SEC-bearing female mice (n=40) were divided into 4 equal groups. Mice were given
rhLF orally (100mg/kg/mouse) daily and/or EPI i.p (8mg/kg/mouse). The experiment lasted 14
days, after which samples were collected to measure IL-18 and phosphorylated c-Jun N-terminal
kinase (p-JNK) by ELISA and p53 gene expression by real-time PCR.

Results:

Administration of rhLF, either alone or combined with EPI, markedly decreased the tumor
volume and increased tumor inhibition rate as well as survival rate compared to either tumor control
group or EPI-mono treated group. In addition, co-administration of rhLF and EPI increased the
level of activated JNKs and expression of p53 in tumor tissues compared to the tumor, control
group, exhibiting their pro-apoptotic properties. Moreover, the combined treatment with rhLF and
EPI elevated IL-18 level in the intestinal mucosa compared to other experimental groups with a
possible immune-enhancing effect.

Conclusion:

Recombinant human lactoferrin exhibited potential anticancer and immune-enhancing
properties in mice with breast cancer. Co-treatment with rhLF and EPI proved to be a promising
strategy in cancer treatment.
Designing and Characterization of Tregitope-Based Multi-Epitope Vaccine
Against Multiple Sclerosis: An Immunoinformatic Approach
Authors: Barazesh Mahdi , Bemani Peyman , Jalili Sajad , Hassanpour Kazem , Faraji Fouziyeh , Gholijani Nasser , Mohammadi Mozafar , Farnoosh Gholamreza
Published: May 2022

Background:

Multiple sclerosis (MS) is a chronic autoimmune disease of the central
nervous system(CNS). It is widely accepted that the development and progression of MS result
from aberrant activation of potentially encephalitogenic reactive-T cells against CNS antigens. The
pathologic roles of both CD4+ (T helper; Th) and CD8+ T cells have been demonstrated in MS lesions.

Objective:

In the present work, we applied a series of bioinformatics tools to design a dendritic cell
(DC)-targeting Tregitope-based multi-epitope vaccine for MS to induce tolerance in pathogenic
myelin-specific T cells.

Methods:

The 3D structure of anti-DEC205 scFv and the remaining part of the vaccine were modeled
by ROSIE Antibody server and ITASSER software, respectively. AIDA web server (ab initio
domain assembly server) was applied to assemble two parts of the vaccine and build the full
construct. Following modeled structure refinement and validation, physicochemical properties, and
allergenicity of the vaccine were assessed. In the final step, in silico cloning was done to ensure
high-level expression in the desired host.

Results:

This vaccine consists of three main parts; 1) Anti-DEC205 scFv antibody, 2) multiepitope
vaccine part composed of multiple pathogenic CD4+, and CD8+ T cell epitopes originated
from multiple known antigens in MS patients, as well as T-regulatory (Treg)-inducing epitopes
(Tregitopes), and 3) vasoactive intestinal peptide (VIP). All parts of the final vaccine were joined
together with the help of proper linkers. After vaccine construction, the three-D structure, as well
as different physicochemical and immunological features of the vaccine were predicted. Finally, in
silico gene cloning was also carried out to assure efficient production of protein vaccine in Escherichia
coli K12 expression strain.

Conclusion:

Computational study revealed that this vaccination can regulate MS disease progression
and even relapse by harnessing pathogenic T cells.
The Effects of Vitamin D3 and Melatonin Combination on Pentylenetetrazole- induced Seizures in Mice
Authors: Mehrzadi Saeed , Hosseinzadeh Azam , Dehdashtian Ehsan , Jafari-Sabet Majid
Published: May 2022

Background:

Epileptic seizures are associated with the overproduction of free radicals in the brain leading to neuronal cell death. Therefore, reduction of oxidative stress may inhibit seizure- induced neuronal cell damage. The current study evaluated the effects of Vit D3 and melatonin and their combination on pentylenetetrazol (PTZ)-induced tonic-clonic seizures in mice.

Methods:

Animals were divided into six groups. Group I was administered with normal saline (0.5 ml, intraperitoneally (i.p.)) on the 15th day of the experiment. Group II was injected with PTZ (60 mg/kg dissolved in 0.5 ml normal saline, i.p) on the 15th day. Groups III-IV were treated with diazepam (4 mg/kg/day), Vit D3 (6000 IU/kg/day), melatonin (20 mg/kg/day), and Vit D3 (6000 IU/kg/day)/melatonin (20 mg/kg/day), respectively, and were then injected with PTZ (60 mg/kg) on the 15th day of the experiment. Immediately after the injection of PTZ on the 15th day, mice were observed for a 30-min period to measure seizure latency and duration. For determination of oxidative stress markers, malondialdehyde (MDA) level and superoxide dismutase (SOD) activity were measured in mouse brains.

Results:

Treatment with Vit D3, melatonin, and Vit D3/melatonin significantly increased seizure latency and decreased seizure duration. The brain level of MDA was lower, and SOD activity was greater than in the PTZ group. Mice treated with Vit D3/melatonin had lower seizure duration than other treated groups.

Conclusions:

The combination of Vit D3 and melatonin may reduce seizure frequency in epileptic patients; this effect may result from various mechanisms, including inhibition of oxidative stress.
Role of G-Proteins and GPCR-Mediated Signalling in Neuropathophysiology
Authors: Kamal Mohammad Amjad , Rahman Md. Mominur , Mim Sadia Afsana , Islam Md. Rezaul , Sultana Nasrin , Ahmed Muniruddin
Published: May 2022

G-protein-coupled receptors (GPCRs) are activated by manifold neurotransmitters, and their
activation, in turn, evokes slow synaptic transmission. They are profoundly related to numerous psychiatric
and neurological disorders such as schizophrenia and Parkinson's disease. The significant malady
indications for GPCR modulators demonstrate a change towards obesity, diabetes, and Alzheimer's
disease, while other central nervous system disorders persist highly represented. GPR52, GPR6,
and GPR8 are recognised as orphan GPCRs, co-exist either with both the dopamine D2 and D1 receptors
in neurons of the basal ganglia or with the dopamine D2 receptor alone, and recommend that between
these orphan receptors, GPR52 has the maximum potential of being a therapeutic psychiatric
receptor. Genetically modified creature models and molecular biological investigations have suggested
that these improved GPCRs could be potential therapeutic psychiatric receptors. In this perspective,
the role of molecular targets in GPCR-mediated signalling has been discussed that would be novel
drug design and discovery options for a scientist to elaborate previous knowledge with modern techniques.
Pengembangan media pembelajaran STEM dengan augmented reality untuk meningkatkan kemampuan spasial matematis siswa
Authors: Arifin Alif Maulana , Pujiastuti Heni , Sudiana Ria
Published: May 2022

Penelitian ini bertujuan untuk menghasilkan produk berupa media pembelajaran berbasis STEM dengan augmented reality yang valid, praktis, dan efektif dalam meningkatkan kemampuan spasial matematis siswa. Jenis penelitian ini adalah penelitian pengembangan dengan model ADDIE yang diadaptasi dari Lee Owens yang meliputi yaitu analyze, design, development, implementation, and evaluation. Implementasi media yang dikembangkan dilakukan di SMK Negeri 1 Pandeglang dengan melibatkan 25 orang siswa kelas XII RPL 2 dan 2 orang guru matematika. Hasil dari penelitian ini adalah sebuah media pembelajaran yang diberi nama “Artic” yang valid, praktis, dan efektif. Media pembelajaran dinyatakan valid berdasarkan penilaian dari tiga orang ahli media dan tiga orang ahli pendidikan, dengan persentase kevalidan secara berturut-turut sebesar 91% (sangat layak) dan 93% (sangat layak). Media dinyatakan praktis berdasarkan penilaian oleh guru dengan persentase penilaian sebesar 85% (sangat praktis) dan penilaian dari siswa dengan persentase penilaian sebesar 94% (sangat baik). Keefektifan media berdasarkan skor n-gain sebesar 0,42 dengan interpretasi terdapat peningkatan pada kategori sedang. Berdasarkan hasil tersebut, maka media pembelajaran yang dikembangkan layak digunakan untuk meningkatkan kemampuan spasial matematis siswa. AbstractThis study aimed to produce a product in the form of a learning media based on STEM with Augmented Reality which was valid, practical, and effective to improve students’ mathematical spatial abilities. This study was a research and development with the ADDIE model adapted from Lee Owens which includes analyzing, design, development, implementation, and evaluation. The implementation of the developed media was carried out at SMK (vocational high school) Negeri 1 Pandeglang, Indonesia by involving 25 students of class XII RPL 2 and two mathematics teachers. The results of this study were a learning media that was given the name “Artic” which was valid, practical, and effective. Learning media was declared valid based on the assessment of three media experts and three educational experts, with a percentage of validity respectively 91% (very feasible) and 93% (very feasible). The media was declared practical based on the assessment by the teacher with a percentage score of 85% (very practical) and an assessment by students with a percentage score of 94% (very good). The effectiveness of the media based on the n-gain score of 0.42 with the interpretation there was an increase in the medium category. Based on these results, the learning media developed was appropriate to be used to improve students’ mathematical spatial abilities.
Feasibility of Using Games to Improve Healthy Lifestyle Knowledge in Youth Aged 9-16 Years at Risk for Type 2 Diabetes: Pilot Randomized Controlled Trial
Authors: Maddison Ralph , Baghaei Nilufar , Calder Amanda , Murphy Rinki , Parag Varsha , Heke Ihirangi , Dobson Rosie , Marsh Samantha
Published: May 2022

Background

Mobile games can be effective and motivating tools for promoting children’s health.

Objective

We aimed to determine the comparative use of 2 prototype serious games for health and assess their effects on healthy lifestyle knowledge in youth aged 9-16 years at risk for type 2 diabetes (T2D).

Methods

A 3-arm parallel pilot randomized controlled trial was undertaken to determine the feasibility and preliminary effectiveness of 2 serious games. Feasibility aspects included recruitment, participant attitudes toward the games, the amount of time the participants played each game at home, and the effects of the games on healthy lifestyle and T2D knowledge. Participants were allocated to play Diabetic Jumper (n=7), Ari and Friends (n=8), or a control game (n=8). All participants completed healthy lifestyle and T2D knowledge questionnaires at baseline, immediately after game play, and 4 weeks after game play. Game attitudes and preferences were also assessed. The primary outcome was the use of the game (specifically, the number of minutes played over 4 weeks).

Results

In terms of feasibility, we were unable to recruit our target of 60 participants. In total, 23 participants were recruited. Participants generally viewed the games positively. There were no statistical differences in healthy lifestyle knowledge or diabetes knowledge over time or across games. Only 1 participant accessed the game for an extended period, playing the game for a total of 33 min over 4 weeks.

Conclusions

It was not feasible to recruit the target sample for this trial. The 2 prototype serious games were unsuccessful at sustaining long-term game play outside a clinic environment. Based on positive participant attitudes toward the games, it is possible to use these games or similar games as short-term stimuli to engage young people with healthy lifestyle and diabetes knowledge in a clinic setting; however, future research is required to explore this area.

Trial Registration

Australia New Zealand Clinical Trials Registry ACTRN12619000380190; https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=377123
Evaluation of Online Near-Peer Teaching for Penultimate-Year Objective Structured Clinical Examinations in the COVID-19 Era: Longitudinal Study
Authors: Shah Savan
Published: May 2022

Background

The benefits of near-peer learning are well established in several aspects of undergraduate medical education including preparing students for Objective Structured Clinical Examinations (OSCEs). The COVID-19 pandemic has resulted in a paradigm shift to predominantly online teaching.

Objective

This study aims to demonstrate the feasibility and benefits of an exclusively online near-peer OSCE teaching program in a time of significant face-to-face and senior-led teaching shortage.

Methods

A teaching program was delivered to penultimate-year students by final-year students at Manchester Medical School. Program development involved compiling a list of salient topics and seeking senior faculty approval. Teachers and students were recruited on Facebook. In total, 22 sessions and 42 talks were attended by 72 students and taught by 13 teachers over a 3-month period. Data collection involved anonymous weekly questionnaires and 2 separate anonymous student and teacher postcourse questionnaires including both quantitative and qualitative components.

Results

On a scale of 1-10, students rated the quality of the program highly (mean 9.30, SD 1.15) and felt the sessions were highly useful in guiding their revision (mean 8.95, SD 0.94). There was a significant increase in perceived confidence ratings after delivery of the program (P<.001). Teachers felt the program helped them better understand and retain the subject material taught (mean 9.36, SD 0.81) and develop skills to become effective clinical teachers (mean 9.27, SD 0.79).

Conclusions

This is the first study demonstrating the efficacy of a near-peer OSCE teaching program delivered exclusively online. This provides an exemplary framework for how similar programs should be encouraged given their efficacy and logistical viability in supplementing the undergraduate curriculum.
A recurrent neural network model predicts activated partial thromboplastin time after treatment with heparin - a retrospective study (Preprint)
Authors: Boie Sebastian Daniel , Engelhardt Lilian Jo , Coenen Nicolas , Giesa Niklas , Rubarth Kerstin , Menk Mario , Balzer Felix
Published: May 2022

BACKGROUND

Anticoagulation therapy with Heparin is a frequent treatment in intensive care units, which is monitored by activated partial thromboplastin clotting time (aPTT). It has been demonstrated that reaching an established anticoagulation target within 24 hours is associated with favorable outcomes. However, patients respond to heparin differently and reaching the anticoagulation target can be challenging. Machine learning algorithms may potentially support clinicians with improved dosing recommendations.

OBJECTIVE

This study evaluates a range of machine learning algorithms on their capability of predicting the patients’ response to heparin treatment. In the present analysis, we apply, for the first time, a model that considers time series.

METHODS

We extracted patient demographics, laboratory values, dialysis and ecmo treatments, and scores from the hospital information system. We set up a regression task for predicting the aPTT laboratory values 24 hours after continuous heparin infusion and evaluate seven different machine learning models. We consider all data before and within the first 12 hours of continuous heparin infusion as features and predict the aPTT value after 24 hours.

RESULTS

The distribution of aPTT in our cohort of 5926 hospital admissions is highly skewed. While most patients show aPTT values below 75 s, some outliers show much higher aPTT values. A recurrent neural network that consumes time series of features shows the highest performance on the test set.

CONCLUSIONS

A recurrent neural network that uses time series of features instead of only static and aggregated features, shows the highest performance of predicting aPTT after heparin treatment.
Elderly wheelchair users recommend age-friendly design improvements to a mHealth wheelchair maintenance application (Preprint)
Authors: Boccardi Alyssa , Wu Fangzheng , Pearlman Jon , Mhatre Anand
Published: May 2022

BACKGROUND

Preventative wheelchair maintenance reduces wheelchair failures and prevents user consequences. The authors are developing a smartphone app that alerts users when maintenance is due to encourage maintenance practices and compliance.

OBJECTIVE

Collect qualitative and quantitative data on older adults' interaction experience with the WheelTrak app and perceived barriers to maintenance.

METHODS

Interviews with stakeholders, including mobility device users, to generate app specifications. Usability testing and semi-structured interviews were conducted with elderly wheelchair and scooter users (n=12). The System Usability Scale (SUS) was administered.

RESULTS

Nine participants agreed they would use WheelTrak for preventative maintenance. WheelTrak scored an average SUS score of 60.5. Four overarching themes were identified: 1) WheelTrak app improvements, 2) Barriers to maintenance, 3) Consequences related to mobility device failure, and 4) Smart technology use and acceptance. Older adults preferred simplicity, readability, personalization and educational resources in the app for better usability. Barriers to maintenance pertain to health issues and lack of maintenance knowledge among the elderly.

CONCLUSIONS

The WheelTrak app has close to average system usability. This study highlighted age-friendly design improvements to the app, making it easier to use and adopt by elderly wheelchair users. Additional usability testing will be conducted following app revision.
Morphofunctional parallels of the stomach in patients with chronic atrophic gastritis
Authors: Mosyichuk L.M. , Gaydar Yu.A. , Klenina I.A. , Petishko O.P.
Published: May 2022

Background. Despite some progress in addressing gastric cancer, a marked reduction in morbidity in all developed countries, the search for changes that precede carcinogenesis remains relevant and far from complete. The purpose of the study: to assess the content of aggressive and protective factors of gastric juice and oral fluid in comparison with morphological changes in patients with chronic atrophic gastritis. Materials and methods. The study included 56 patients: group I included 12 patients with atrophic changes of the gastric mucosa of varying severity, groupII— 24 patients with atrophic changes diagnosed with intestinal metaplasia only in the antrum of the stomach, in group III group— 20 patients with intestinal metaplasia in the body and antrum of the stomach. The control group consisted of 16 healthy individuals. Assessment of the degree of atrophy and inflammation in the gastric mucosa was performed with the subsequent determination of integral indicators— stage and degree of atrophy according to the OLGA system and metaplasia— according to the OLGIM system. According to the morphometric study of sections using a light microscope XSP-139TP (“Ulab“, Ukraine) calculated indicators: nuclear-cytoplasmic ratio, the ellipticity of the nuclei. The content of glycoproteins, sialic acids, fucose, hexosamines was determined in gastric juice and oral fluid. Results. Morphometric study showed a significant decrease in the nuclear-cytoplasmic ratio to (0.12±0.04)% in patients of group III. The coefficient of ellipticity of the nuclei of native cells decreased with the development of intestinal metaplasia: in group I it was equal to (0.76±0.04)%, for group II it was (0.65±0.11)%, and for group III— 0.41±0.12)%. In patients with intestinal metaplasia in 82% of cases with a 3-fold increase in the content of sialic acids in gastric juice was diagnosed with an increase in hexosamines by 35%, while patients with only atrophic changes in the stomach showed a decrease in hexosamines 2 times compared to controls (p<0.05). The spread of intestinal metaplasia in the stomach is associated with an increase in the amount of glycoproteins and hexosamines in the oral fluid of patients, while sialic acids in this biological fluid are increased in all study groups. There are direct relationships between the presence of intestinal metaplasia in the body of the stomach and the content of glycoproteins in both gastric juice (r=0.446, p=0.008) and in oral fluid (r=0.378, p=0.021). The relationship between the content of sialic acids in gastric juice with the degree and stage of gastritis by OLGA (r=0.431, p<0.01; r=0.482, p<0.01), the level of hexosamines in oral fluid with the coefficient of ellipticity of the nuclei (r=0.447, p=0,037). Conclusions. A comprehensive study of morpho-functional changes in the stomach and the ratio of aggressive and projective factors of oral fluid will determine the risk groups of patients with precancerous conditions.
ANXIETY AMONG THE URBAN WORKING WOMEN AND HOMEMAKERS: A QUESTIONNAIRE-BASED SURVEY
Authors: JANA NIKTA , NAYAKA MONIKA , DEVERSHETTY SHRAVANI , GAJJALA PRAVEEN KUMAR , KAMDAR PRIYANKA , PAL CHANDANA
Published: May 2022

Objective: Increasing complexity of life is associated with a parallel increase in anxiety, particularly among women due to their physiology and hormonal influence. This study evaluated and compared the anxiety status of working women and homemakers.
Methods: The target population for this survey-based study were selected randomly; responses for the questionnaire was assessed and scoring was done with the help of a scoring key. PSYCOM anxiety test was used for the assessment of anxiety.
Results: Sixty women (30 in each groups) participated in the survey; anxiety was common among those in the age group of 21−40 years. Twenty-two (36.66%) had no symptoms of anxiety, of whom 13 (59.01%) were working women. Anxiety levels were high among home makers (70% vs. working women 56.66%). Mild (60.53%), moderate (28.95%), and moderately severe anxiety was common among women (total 34/38, 89.47%). Among homemakers, nine had no anxiety, 13 had mild, six had moderate, and one each had moderately severe and severe anxiety, respectively. Among working women, 13 had no anxiety, ten had mild, five had moderate, and two had moderately severe anxiety. Anxiety was less among working women and of milder degree in younger working women, when present. Moderately severe and severe level of anxiety were not reported in younger women in both groups. Homemakers, particularly older women experienced mild anxiety to a greater extent; both working and non-working older women experienced moderate and moderately severe anxiety in equal proportions.
Conclusion: Mild and moderate anxiety is more common among women; homemakers, particularly older women are at a high risk.
Comparative Study on Soil Microbes from Muthalamada Mango Farms
Authors: C Mohammed Nizam , S Nisy , Narayan Vishnu , Abbaz Mohammed , M Harikrishnan. , M M Athira , U Vinya
Published: May 2022

Abstract: Use of chemical pesticide to increase crop yield, especially in high income generating crops are common all over the world. Adverse effects of chemical pesticides on humans are a well-known. Application of chemical pesticides change the bio diversity starting from agricultural field and eventually making its way to natural ecosystem. The harmful effects of pesticide are often forgot because how the agroindustry and pesticides markets generate huge incomes since it is a multibillion-dollar market. The chemical pesticide changes the bio diversity of soil, affecting earthworms, nematodes, protozoa to various microbial communities which are essential for proper functioning of ecosystems. Microbial community is essential part of soil ecosystem, they directly influence various nutrient cycles and nutritional values of soil. The use of chemical pesticides affects the natural enzymes and functioning of bacteria and inhibits the production of useful enzymes and secondary metabolites, which helps in crop yield. In this study, soil samples from three mango farms were collected and the microbial load were analyzed. The study revealed that the mango farm which adopted organic farming technique combined with need based and approved amounts of pesticides having more soil bacteria compared to very less amount of microbial load in mango farm using chemical pesticides in Muthalamada, Mango City of Kerala. Use of organic farming along with the integration of approved pesticides in a controlled manner on agriculture fields improves crop yield without any harmful effects to the environment. Keywords: Mango, Organic farming, Pesticides, Muthalamada.
MediSolution Innovate Your Health Care
Authors: Singh Subhankar , Alam Tariq , Sharma Ashwani , Sharma Himanshu , Singh Akshay Pratap
Published: May 2022

Abstract: Online Bussiness is growing day by daysince the pandemic struck the world. People are shifting to online websites for there many work for example ordering goods and services, booking appointment doing business. Many companies are changing there ways and establishing and online market to reach more and more people. The connectivity online market provide is crucial for reaching large numberof people and delivering there services. During pandemic one of the most essentialweb services was health care system. Because people use to wait in line for doctors appointment but with an online website they can book a doctor With click of a button instantly from there home . Thispaper address one of those healthcare system which permits and instance appointment of doctors and joining government scheme to help the patient . Inthis paper we have described an idea how a web service platform will remove the needof waiting lines and converting everything digital. This web service store patient data for future use so that the doctors can easily know about the patient without any hastle and by invoving the government scheme anyone can take advantage of it by knowing various types of scheme that canhelp them financially and they can also know the eligibility.
Effect on Efficiency of Air Filter Element at Steady and Dynamic Condition
Authors: Gade Pranav M. , Chakote Subhash M. , Garkhedkar Ganesh G.
Published: May 2022

Abstract: For the better protection of the engine, required highly efficient air. Primary feature of engine air filter element is the dust holding capacity, in the parameters major factor is efficiency when we are selecting an engine air filter media or element. For deliver higher filtration performances constantly being challenge in automotive engine air filters. There is need to protect the engine from dust particle which effects on the performance and life of the engine, abrasive contaminants are increasing to achieve longer engine life, for improved engine performance. The innovative air filter designs are required to maximize filtration property and better performance. The performance of the engine as well as life of the engine are based on filtration efficiency of air filter. In this study we are discussed about filtration efficiency of the air filter. In this experiment we study the effect of the vibration on the air filter media performance as compare with the steady performance of the air filter media or assembly at steady condition. One is at steady condition and second is dynamic condition. Both tests are performing in the laboratory according to ISO5011 standard. Keywords: Air Filter, Filtration, Performance, Vibration, Air Filter Media.
Youth Perception on Agricultural Sector (Case Study in Watukenongo, Pungging, Mojokerto).
Authors: Bagus Setiawan Dio , Ibrahim Jabal Tarik , Amir Nur Ocvanny
Published: May 2022

This study aims at analyzing youth perceptions on agricultural sector and analyzing factors affecting youth perceptions on agricultural sector in Watukenongo village. 91 respondents were involved in this study as sample. Simple random sampling technique was employed in this study. All respondents are youths in Watukenongo village, Pungging, Mojokerto Regency. For data analysis method, the SEM-PLS (Partial Least Square) technique with the help of WarpPLS 7.0 software was implemented. The results showed that the youth perception on agricultural sector was poor in terms of enthusiasm in doing or continuing to farm; it was good in terms of income satisfaction resulted from farming and in terms of self-ability in cultivating agricultural land; and it was very good in terms of pleasure in doing farming activities. The obtained value of R-square is 0.690. The factors affecting youth perception agricultural sector were (1) income, (2) land, and (3) education. Meanwhile, social status and age did not have a significant effect.
APPLICATION OF LIQUID ORGANIC FERTILIZER AND NPK Blue ON THE GROWTH OF PEANUT (Arachis hypogea L.)
Authors: Sebayang Nico Syahputra , Tambunan Syariani , Suhadi Suhadi
Published: May 2022

Peanuts make a significant contribution in meeting the needs of legumes, especially in Southeast Aceh Regency. This research was conducted in Kisam Village, Bambel District, Southeast Aceh, with an altitude of ± 220 m above sea level. The aim of the study was to determine the application of liquid organic fertilizer and the dose of NPK fertilizer on the growth and development of peanut (arachis Hypogea L.) plants. The design used in this study was a Randomized Block Design (RAK) which was arranged in a factorial manner with 9 treatment combinations with 3 (three) replications. The treatment consisted of 2 (two) factors, namely the first treatment of liquid organic fertilizer (P1) 2cc/l water, (P2) 4 cc/l water and (P3) 8 cc/l water, while the second factor was the dose of NPK fertilizer (N1 ) 1.25 g/plant, (N2) 1.75gr/plant and (N3) 2.25 g/plant. The observed variables were plant height, number of branches, number of petioles and number of leaves. The results showed that the application of liquid organic fertilizer (P1) gave the best results on plant height, number of branches, number of petioles and number of leaves. Dosage of NPK (N3) fertilizer gave the best results on plant height, number of leaves. The interaction of treatment with liquid organic fertilizer (P1) and dose of NPK fertilizer (N3) gave the best effect on the growth and production of peanut plants. The results of the analysis of variance showed that there was no significant interaction between the treatment of giving liquid organic fertilizer (Pocnasa) and chemical fertilizer (NPK Biru) to all observed variables.
Pengelolaan Konten Media Sosial Instagram @info_tuban sebagai Sarana Informasi Tuban
Authors: Septyami Diah Eka , Zuhri Saifuddin
Published: May 2022

Infotuban uses social media Instagram as a means to share various information with its target audience. The purpose of this study is to find out how the management of social media content on Instagram @info_tuban includes thestages share, optimize, manage, and engage. This research uses a qualitative descriptive research type. Data collection techniques through interviews, observation, and literature study. The informant determination technique used in this research is purposive sampling technique. This study uses two sources of data, namely primary data obtained from observations and interviews and secondary data obtained through books, internet, literature. The results show that in the share stage @info_tuban uses Instagram as a means of delivering effective and communicative information to build public trust. At the optimize stage, to find out what the public is talking about @info_tuban using the manual method, namely through the Instagram story and hashtag features. In thestage, manage @info_tuban has carried out several activities. However, at this stage @info_tuban does not use social media tools. The last stage is engage, @info_tuban has collaborated with people who have influence. To reach the target audience, it is done by regularly posting content. Keywords: content, instagram, information, infotuban
Strategi Komunikasi Badan Kepegawaian dan Pengembangan Sumber Daya Manusia dalam Digitalisasi Pelayanan Kenaikan Pangkat Pegawai Negeri Sipil
Authors: Fahmi Rizal Fahmi
Published: May 2022

This study aims to analyze the communication strategy carried out by the Personnel and Human Resources Development Agency in disseminating messages about digitizing promotion services within the Pidie Jaya Regency Government. The research uses a qualitative research approach with case study methods to analyze problems related to communication strategies formulated by organizations. The research results indicate that the communicators chosen to convey messages to the target audience have credibility, the message has been designed in such a way, the channel used is a personal channel face-to-face through technical guidance and consultation and by using the media but the media utilization is still not optimal and unable to reach the overall target audience. The communicant is all civil servants in the Pidie Jaya Regency Government and the effects are limited to changes in knowledge and changes in attitudes. Obstacles in delivering messages are found both in the communicator and the communicant, namely barriers in the delivery process (process barriers), semantic barriers (semantics barriers), and psycho-social barriers (psychosocial barriers) caused by the culture that is already attached to the audience, communicators and communicants are trying to overcome these various obstacles by using personal communication channels both face-to-face and through the media.