bluuebunny/crossref_metadata_2025 huggingface dataset:
Hubungan Pengetahuan dan Sikap Ibu Nifas terhadap Praktik Pemenuhan Kebutuhan Dasar Masa Nifas
Authors: Umaroh Umaroh , Suparmi Suparmi , Musthofa Syamsulhuda Budi
Published: July 2024

Selama masa nifas, tubuh wanita mengalami perubahan hormonal, fisik, dan emosional yang signifikan. Ada 3 jenis kebutuhan dasar yang harus dipenuhi pada masa ini, meliputi kebutuhan aktivitas, kebutuhan istirahat, dan kebutuhan nutrisi. Penelitian ini mempunyai tujuan untuk mengetahui hubungan dari pengetahuan, sikap dan pemenuhan kebutuhan dasar masa nifas. Penelitian ini menggunakan pedekatan cross-sectional. Populasi penelitian ini adalah seluruh ibu nifas normal pada Puskesmas dan Praktik Bidan Mandiri di Kota Semarang dan sekitarnya. Namun, penelitian ini memilih 30 ibu nifas sebagai responden dengan teknik simple random sampling. Hasil dari penelitian ini menunjukkan hubungan yang paling berpengaruh adalah hubungan antara sikap dengan praktik istirahat dan pengetahuan dengan praktik nutrisi yang memiliki nilai p 0,001. Nilai p hubungan pengetahuan dengan praktik istirahat adalah 0,002, hubungan sikap dengan praktik aktivitas 0,003, hubungan sikap dengan praktik nutrisi 0,028, dan hubungan pengetahuan dengan praktik aktivitas 0,035. Ibu nifas sebaiknya lebih memahami kebutuhan dasar pada masa nifas, sehingga segala hal dasar yang dibutuhkan ibu nifas dapat terpenuhi dengan baik.
Application of ISAK 35 in the Presentation of Financial Statements of Non-Profit Entities in the Catholic Church
Authors: Watugigir Indri Fadila Veronica , Kurniawan Rachman , Sahusilawane Wildoms
Published: July 2024

This study aims to determine the form of existing financial reporting and also to determine the implementation of financial reporting in the Catholic Church of St. Joseph Rumah Tiga and St. John the Evangelist Catholic Church Masohi based on ISAK No. 35. The method used is descriptive qualitative. Data sources using primary and secondary data were collected by researchers using interviews, observation, and documentation techniques. In this study, researchers conducted data analysis by collecting data, reducing, testing the validity of the data, and presenting until they could draw a conclusion. The results showed that the financial records of the Catholic Church of St. Joseph's Rumah Tiga and St. John the Evangelist Catholic Church Masohi still use a simple method. Financial recording is only carried out in the event of cash in and cash out (cash base). Then the preparation of financial statements of the Catholic Church of St. Joseph Rumah Tiga and St. John the Evangelist Catholic Church Masohi prepared based on ISAK No. 35 resulted in more structured and more detailed financial statements related to cash in and cash out.
Performance of The White-Rumped Shama (Copysychus malabaricus) Reproduction by Providing Eurycoma longifolia at The Captivity
Authors: Pratomo Hurip , Pramono Taufik Budhi
Published: July 2024

This research aims to determine the reproductive capacity of the White-Rumped Shama provided by Eurycoma longifolia in terms of duration of couple introduction or adjustment period, duration of mating period until laying eggs, number of eggs, number of hatched eggs, and number of survived baby animals until 5 days of living. The laboratory experimental method is performed in the White-Rumped Shama captivity in Bogor. Before the treatment, the birds are matched in each bird cage. The White-Rumped Shamas are divided randomly into 2 groups, each of which consists of 5 pairs (5 repetitions). The first group (K1) as a control, male magpie parents were given the treatment of drinking distilled water after fasting for one hour every morning, Group 2 (K2) as the Eurycoma longifolia treatment group, male White-Rumped Shama parents were given the optimal dose of Eurycoma longifolia, 9 mg/100 of the body weight every morning for 5 days which was converted from the results of research on the treatment of giving Eurycoma longifolia to white rats. Statistical analysis of two tailed T test, Levene's test on data between the control group (K1) and the Eurycoma longifolia group (K2) showed that; Behavior: Jumping, flying, perching on the perchs, approaching the female, chirping, singing, crouching while near to the female while making a distinctive chirp, eating and drinking, duration of time until laying eggs in the mating cage (days), number of eggs in the nest (items ). The number of hatched eggs from those hatched (grains), the number of offspring that survived to the age of 5 days (tails), there were no significant differences between the control group (K1) and the Eurycoma longifolia treatment group (K2).
The Phoenix of stem cells: pluripotent cells in adult tissues and peripheral blood
Authors: Cancedda Ranieri , Mastrogiacomo Maddalena
Published: July 2024

Pluripotent stem cells are defined as cells that can generate cells of lineages from all three germ layers, ectoderm, mesoderm, and endoderm. On the contrary, unipotent and multipotent stem cells develop into one or more cell types respectively, but their differentiation is limited to the cells present in the tissue of origin or, at most, from the same germ layer. Multipotent and unipotent stem cells have been isolated from a variety of adult tissues, Instead, the presence in adult tissues of pluripotent stem cells is a very debated issue. In the early embryos, all cells are pluripotent. In mammalians, after birth, pluripotent cells are maintained in the bone-marrow and possibly in gonads. In fact, pluripotent cells were isolated from marrow aspirates and cord blood and from cultured bone-marrow stromal cells (MSCs). Only in few cases, pluripotent cells were isolated from other tissues. In addition to have the potential to differentiate toward lineages derived from all three germ layers, the isolated pluripotent cells shared other properties, including the expression of cell surface stage specific embryonic antigen (SSEA) and of transcription factors active in the early embryos, but they were variously described and named. However, it is likely that they are part of the same cell population and that observed diversities were the results of different isolation and expansion strategies. Adult pluripotent stem cells are quiescent and self-renew at very low rate. They are maintained in that state under the influence of the “niche” inside which they are located. Any tissue damage causes the release in the blood of inflammatory cytokines and molecules that activate the stem cells and their mobilization and homing in the injured tissue. The inflammatory response could also determine the dedifferentiation of mature cells and their reversion to a progenitor stage and at the same time stimulate the progenitors to proliferate and differentiate to replace the damaged cells. In this review we rate articles reporting isolation and characterization of tissue resident pluripotent cells. In the attempt to reconcile observations made by different authors, we propose a unifying picture that could represent a starting point for future experiments.
Correspondence between multiple signaling and developmental cellular patterns: a computational perspective
Authors: Eidi Zahra , Khorasani Najme , Sadeghi Mehdi
Published: July 2024

The spatial arrangement of variant phenotypes during stem cell division plays a crucial role in the self-organization of cell tissues. The patterns observed in these cellular assemblies, where multiple phenotypes vie for space and resources, are largely influenced by a mixture of different diffusible chemical signals. This complex process is carried out within a chronological framework of interplaying intracellular and intercellular events. This includes receiving external stimulants, whether secreted by other individuals or provided by the environment, interpreting these environmental signals, and incorporating the information to designate cell fate. Here, given two distinct signaling patterns generated by Turing systems, we investigated the spatial distribution of differentiating cells that use these signals as external cues for modifying the production rates. By proposing a computational map, we show that there is a correspondence between the multiple signaling and developmental cellular patterns. In other words, the model provides an appropriate prediction for the final structure of the differentiated cells in a multi-signal, multi-cell environment. Conversely, when a final snapshot of cellular patterns is given, our algorithm can partially identify the signaling patterns that influenced the formation of the cellular structure, provided that the governing dynamic of the signaling patterns is already known.
Prenatal cigarette smoke exposure sensitizes acetaminophen-induced liver injury by modulating miR-34a-5p in male offspring mice
Authors: Yang Daram , Jeong Hyuneui , Kim Min-Seok , Oh Sang-Ik , Lee Kyuhong , Kim Jong-Won , Kim Bumseok
Published: July 2024

Introduction: Cigarette smoke (CS) exacerbates the severity of diseases not only in lungs, but also in systemic organs having no direct contact with smoke. In addition, smoking during pregnancy can have severe health consequences for both the mother and the fetus. Therefore, our aim was to evaluate effects of prenatal exposure to CS on acetaminophen (APAP)-induced acute liver injury (ALI) in offspring.

Methods: Female C57BL/6 mice on day 6 of gestation were exposed to mainstream CS (MSCS) at 0, 150, 300, or 600 μg/L for 2 h a day, 5 days a week for 2 weeks using a nose-only exposure system. At four weeks old, male offspring mice were injected intraperitoneally with a single dose of APAP at 300 mg/kg body weight to induce ALI.

Results: Maternal MSCS exposure significantly amplified pathological effects associated with ALI as evidenced by elevated serum alanine aminotransferase levels, increased hepatocellular apoptosis, higher oxidative stress, and increased inflammation. Interestingly, maternal MSCS exposure reduced microRNA (miR)-34a-5p expression in livers of offspring. Moreover, treatment with a miR-34a-5p mimic significantly mitigated the severity of APAP-induced hepatotoxicity. Overexpression of miR-34a-5p completely abrogated adverse effects of maternal MSCS exposure in offspring with ALI. Mechanistically, miR-34a-5p significantly decreased expression levels of hepatocyte nuclear factor 4 alpha, leading to down-regulated expression of cytochrome P450 (CYP)1A2 and CYP3A11.

Discussion: Prenatal exposure to MSCS can alter the expression of miRNAs, even in the absence of additional MSCS exposure, potentially increasing susceptibility to APAP exposure in male offspring mice.
Age and dose dependent changes to the bone and bone marrow microenvironment after cytotoxic conditioning with busulfan
Authors: Abbasizadeh Nastaran , Burns Christian S. , Verrinder Ruth , Ghazali Farhad , Seyedhassantehrani Negar , Spencer Joel A.
Published: July 2024

Preparative regimens before Hematopoietic Cell Transplantation (HCT) damage the bone marrow (BM) microenvironment, potentially leading to secondary morbidity and even mortality. The precise effects of cytotoxic preconditioning on bone and BM remodeling, regeneration, and subsequent hematopoietic recovery over time remain unclear. Moreover, the influence of recipient age and cytotoxic dose have not been fully described. In this study, we longitudinally investigated bone and BM remodeling after busulfan treatment with low intensity (LI) and high intensity (HI) regimens as a function of animal age. As expected, higher donor chimerism was observed in young mice in both LI and HI regimens compared to adult mice. Noticeably in adult mice, significant engraftment was only observed in the HI group. The integrity of the blood-bone marrow barrier in calvarial BM blood vessels was lost after busulfan treatment in the young mice and remained altered even 6 weeks after HCT. In adult mice, the severity of vascular leakage appeared to be dose-dependent, being more pronounced in HI compared to LI recipients. Interestingly, no noticeable change in blood flow velocity was observed following busulfan treatment. Ex vivo imaging of the long bones revealed a reduction in the frequency and an increase in the diameter and density of the blood vessels shortly after treatment, a phenomenon that largely recovered in young mice but persisted in older mice after 6 weeks. Furthermore, analysis of bone remodeling indicated a significant alteration in bone turnover at 6 weeks compared to earlier timepoints in both young and adult mice. Overall, our results reveal new aspects of bone and BM remodeling, as well as hematopoietic recovery, which is dependent on the cytotoxic dose and recipient age.
Desmoplastic small round cell tumor: from genomics to targets, potential paths to future therapeutics
Authors: Magrath Justin W. , Espinosa-Cotton Madelyn , Flinchum Dane A. , Sampath Shruthi Sanjitha , Cheung Nai Kong , Lee Sean B.
Published: July 2024

Desmoplastic Small Round Cell Tumor (DSRCT) is a highly aggressive pediatric cancer caused by a reciprocal translocation between chromosomes 11 and 22, leading to the formation of the EWSR1::WT1 oncoprotein. DSRCT presents most commonly in the abdominal and pelvic peritoneum and remains refractory to current treatment regimens which include chemotherapy, radiotherapy, and surgery. As a rare cancer, sample and model availability have been a limiting factor to DSRCT research. However, the establishment of rare tumor banks and novel cell lines have recently propelled critical advances in the understanding of DSRCT biology and the identification of potentially promising targeted therapeutics. Here we review model and dataset availability, current understanding of the EWSR1::WT1 oncogenic mechanism, and promising preclinical therapeutics, some of which are now advancing to clinical trials. We discuss efforts to inhibit critical dependencies including NTRK3, EGFR, and CDK4/6 as well as novel immunotherapy strategies targeting surface markers highly expressed in DSRCT such as B7-H3 or neopeptides either derived from or driven by the fusion oncoprotein. Finally, we discuss the prospect of combination therapies and strategies for prioritizing clinical translation.
Prospects for cordycepin biosynthesis in microbial cell factories
Authors: Li Xiang , Jiang Rongshuai , Wang Shenghou , Li Chenyang , Xu Yongping , Li Shuying , Li Qian , Wang Liang
Published: July 2024

Cordycepin, an adenosine analog, exhibits diverse bioactivities and holds significant potential for applications in healthcare and agriculture. Fungi of the genus Cordyceps, such as Cordyceps militaris, can naturally produce cordycepin. Current sources of cordycepin primarily involve extraction from fruiting bodies or isolation from liquid fermentation using C. militaris, presenting challenges such as low production intensity, complex separation and purification systems, and high production costs, limiting industrial feasibility. Recent advancements have witnessed the utilization of various fungal chassis cells to successfully engineer heterologous biosynthetic platforms for cordycepin, such as Saccharomyces cerevisiae and unconventional yeasts, offering advantages of high yield, short fermentation cycles, and a broad substrate spectrum. This mini review summarizes the biosynthetic pathways of cordycepin and focused on the comparison of the characteristics, advantages, current performance and prospects for the microbial cell factories, analyzing potential targets for metabolic pathway modification and giving strategies in both genetic engineering and process engineering to enhance production intensity. The mini review particularly emphasizes the crucial role of chassis cell stress tolerance to the toxic product in determining cordycepin yield and highlights the urgent need for high-throughput screening methods for high-yield strains.
Theoretical study of the antioxidant mechanism and structure-activity relationships of 1,3,4-oxadiazol-2-ylthieno[2,3-d]pyrimidin-4-amine derivatives: a computational approach
Authors: Bakheit Ahmed H. , Wani Tanveer A. , Al-Majed Abdulrahman A. , Alkahtani Hamad M. , Alanazi Manal M. , Alqahtani Fahad Rubayyi , Zargar Seema
Published: July 2024

A theoretical thermodynamic study was conducted to investigate the antioxidant activity and mechanism of 1,3,4-oxadiazol-2-ylthieno[2,3-d]pyrimidin-4-amine derivatives (OTP) using a Density Functional Theory (DFT) approach. The study assessed how solvent environments influence the antioxidant properties of these derivatives. With the increasing prevalence of diseases linked to oxidative stress, such as cancer and cardiovascular diseases, antioxidants are crucial in mitigating the damage caused by free radicals. Previous research has demonstrated the remarkable scavenging abilities of 1,3,4-oxadiazole derivatives, prompting this investigation into their potential using computational methods. DFT calculations were employed to analyze key parameters, including bond dissociation enthalpy (BDE), ionization potential (IP), proton dissociation enthalpy (PDE), and electron transfer enthalpy (ETE), to delineate the antioxidant mechanisms of these compounds. Our findings indicate that specific electron-donating groups such as amine on the phenyl rings significantly enhance the antioxidant activities of these derivatives. The study also integrates global and local reactivity descriptors, such as Fukui functions and HOMO-LUMO energies, to predict the stability and reactivity of these molecules, providing insights into their potential as effective synthetic antioxidants in pharmaceutical applications.
Dynamic evolution of amplitude and position of geomagnetic secular acceleration pulses since 2000
Authors: Bai Chunhua , Gao Guoming , Wen Limin , Kang Guofa
Published: July 2024

Recent studies on the behavior of geomagnetic secular acceleration (SA) pulses have provided a basis for understanding the dynamic processes in the Earth’s core. This analysis statistically evaluates the evolution of the SA pulse amplitude and position since 2000 by computing the three-year difference in SA with the CHAOS-7 geomagnetic field model (CHAOS-7.17 release). Furthermore, the study explores the correlation between the acceleration pulse amplitude and geomagnetic jerks and the dynamic processes of alternating variation and polarity reversal of pulse patches over time. Research findings indicate that the variation in pulse amplitude at the Core Mantle Boundary (CMB) closely resembles that observed at the Earth’s surface, with an average period of 3.2 years. The timing of peak pulse amplitude aligns with that of the geomagnetic jerk, suggesting its potential utility as a novel indicator for detecting geomagnetic jerk events. The acceleration pulses are the strongest near the equator (2°N) and more robust in the high-latitude region (68°S) of the Southern Hemisphere, indicating that the variation is more dramatic in the Southern Hemisphere. The acceleration pulses fluctuate unevenly in the west-east direction, with characteristics of local variation. In the Western Hemisphere, the pulse patches are distributed near the equator, exhibiting an evident westward drifting mode. The positive and negative patches alternate in time, displaying a polarity reversal in the west-east direction, with an average interval of approximately 32°. These characteristics can be attributed to the rapid magnetic field fluctuations disclosed by the model of stratification at the top of the Earth’s core. In the Eastern Hemisphere, the pulses are weaker between 10°E and 60°E, with the most active pulses occurring around 80°E to 105°E and near 150°E. The pulse patches exhibit a broader distribution in the north-south direction, with relatively strong patches still occurring near 40°N and 40°S. These local variation characteristics match the actual cases of zonal flows and geostrophic Alfvén waves in the Earth’s core.
Genomic characterization of bZIP gene family and patterns of gene regulation on Cercospora beticola Sacc resistance in sugar beet (Beta vulgaris L.)
Authors: Yin Xiao , Liu Yu , Gong Yunhe , Ding Guangzhou , Zhao Chunlei , Li Yanli
Published: July 2024

Sugar beet (Beta vulgaris L.) is one of the most important sugar crops, accounting for nearly 30% of the world’s annual sugar production. And it is mainly distributed in the northwestern, northern, and northeastern regions of China. However, Cercospora leaf spot (CLS) is the most serious and destructive foliar disease during the cultivation of sugar beet. In plants, the bZIP gene family is one of important family of transcription factors that regulate many biological processes, including cell and tissue differentiation, pathogen defense, light response, and abiotic stress signaling. Although the bZIP gene family has been mentioned in previous studies as playing a crucial role in plant defense against diseases, there has been no comprehensive study or functional analysis of the bZIP gene family in sugar beet with respect to biotic stresses. In this study, we performed a genome-wide analysis of bZIP family genes (BvbZIPs) in sugar beet to investigate their phylogenetic relationships, gene structure and chromosomal localization. At the same time, we observed the stomatal and cell ultrastructure of sugar beet leaf surface during the period of infestation by Cercospora beticola Sacc (C. beticola). And identified the genes with significant differential expression in the bZIP gene family of sugar beet by qRT-PCR. Finally we determined the concentrations of SA and JA and verified the associated genes by qRT-PCR. The results showed that 48 genes were identified and gene expression analysis indicated that 6 BvbZIPs were significantly differential expressed in C. beticola infection. It is speculated that these BvbZIPs are candidate genes for regulating the response of sugar beet to CLS infection. Meanwhile, the observation stomata of sugar beet leaves infected with C. beticola revealed that there were also differences in the surface stomata of the leaves at different periods of infection. In addition, we further confirmed that the protein encoded by the SA signaling pathway-related gene BVRB_9g222570 in high-resistant varieties was PR1, which is closely related to systemic acquired resistance. One of the protein interaction modes of JA signal transduction pathway is the response of MYC2 transcription factor caused by JAZ protein degradation, and there is a molecular interaction between JA signal transduction pathway and auxin. Despite previous reports on abiotic stresses in sugar beet, this study provides very useful information for further research on the role of the sugar beet bZIP gene family in sugar beet through experiments. The above research findings can promote the development of sugar beet disease resistance breeding.
Packing simulation and analysis applied to a thermoplastic composite recycling process
Authors: Bruneau Awen , Mahé François , Binetruy Christophe , Comas-Cardona Sébastien , Landry Charlotte , Durand Nelly
Published: July 2024

A numerical model of packing applied to rigid objects is presented. It aims at describing a random stack of polymer composite chips in order to model the packing step of an existing recycling technique. The geometric properties of the stack play a major role in the mechanical properties of the recycled products. Short, simple and effective geometric descriptors of the stack are proposed. Their ability to differentiate random stacks is illustrated with an example. Then, a validation is proposed based on experimental data obtained from a bench specially designed for this work. The tests consist in the free fall of square chips. Finally, the developed model is compared to other models (free fall and packing of fibers) in order to enforce its relevance in the simulation of packing of rigid objects.
Recombinant proteins as promising antigens applied to the immunodiagnosis of Chagas disease: a scoping review
Authors: Resende Carlos Ananias Aparecido , Ribeiro Anna Júlia , Gandra Isadora Braga , Silva Kamila Alves , Lopes Lucas da Silva , Barcelos Isabelle Caroline dos Santos , Couto Carolina Alves Petit , de Faria Mariana Teixeira , Pereira Sabrina Paula , Xavier Sandra Rodrigues , Machado Juliana Martins , da Paz Mariana Campos , Chaves Ana Thereza , Coelho Eduardo Antônio Ferraz , Giunchetti Rodolfo Cordeiro , Chávez-Fumagalli Miguel Angel , Dutra Walderez Ornelas , Gonçalves Ana Alice Maia , Galdino Alexsandro Sobreira
Published: July 2024

Chagas disease (CD), caused by the protozoan Trypanosoma cruzi, is an important public health problem, occurring mainly in Latin America. The disease has a major social and economical effect, negatively impacting the life of the infected individuals, and bringing great costs to public health. An early and accurate diagnosis is essential for administration of early treatment. In addition, prognostic tests may aid disease management, decreasing hospitalization costs. However, the serological diagnostic scenario for CD still faces several challenges, making the development of new diagnostic kits a pressing matter. Facing this scenario, several researchers have expanded efforts in developing and testing new antigens, such as recombinant proteins and recombinant multiepitope proteins, with promising results. These recombinant antigens offer several advantages, such as improved sensitivity and specificity, in addition to facilitated scaling. Also, it has been possible to observe a rising number of studies using ELISA and point-of-care platforms, employing these antigens in the past few years. Among them, recombinant proteins were the most applied antigens, demonstrating great capacity to discriminate between positive and negative samples. Although fewer in number, recombinant multiepitope proteins also demonstrated an improved diagnostic performance. Indeed, a great number of studies employing these antigens showed sensitivity and specificity values above 90%, greatly impacting diagnostic accuracy. Nevertheless, despite the good results found, it is still possible to observe some bottlenecks in the development of new antigens, such as the scarcity of tests with sera from the acute phase and the variability of results in different geographic areas. In this sense, aiming to contribute to control and health programs, the continuous search for a more accurate serological diagnosis is essential, both for the acute and chronic phases of the disease.
Candidate proteins interacting with cytoskeleton in cells from the basal airway epithelium in vitro
Authors: Olatona Olusola A. , Choudhury Sayantan R. , Kresman Ray , Heckman Carol A.
Published: July 2024

Introduction: The cytoskeleton consists of actin, microtubules, septins, and intermediate filaments and, in most cells, is anchored to an extracellular matrix. Each cell has a unique arrangement of this network and readjusts it from time to time. To investigate the regulation of these reorganizations, we identified interactors from extracts of four cultured lines representing basal cells from the airway epithelium.

Methods: After immunoprecipitation with an antibody against keratin 17, samples were processed by liquid chromatography and tandem mass spectrometry. Samples not undergoing antibody-mediated capture were processed in parallel.

Results: The main keratins of basal cells, namely, Krt14 (type I) and Krt5 (type II), constituted 67% of the total keratin recovered. Several other intermediate filament proteins, nestin, lamin-B1, and prelamin A/C, were present but not enriched upon immunoprecipitation. Although the class of armadillo-repeat proteins was represented by beta-catenin1 and plakoglobin, other desmosome plaque constituents were absent. Large cytolinkers were represented by the spectraplakin, microtubule-actin cross-linking factor (Macf1), which was enriched by immunoprecipitation, and the plakin, plectin, which was not enriched. Subunits of actin filaments and microtubules, along with numerous proteins associated with them, were recovered in both immunoprecipitated samples and those lacking the capture step. Coefficients of determination were computed based on abundance. The actin-associated proteins, alpha-spectrin and brain-specific angiogenesis inhibitor (Baiaip2l), were modestly correlated with keratin abundance but highly correlated with one another and with the keratin-binding protein, annexin A2. This interaction network resembled the pedestal formed by pathogenic Escherichia coli. Microtubule-associated proteins, dynamin 1-like protein and cytoplasmic dynein 1 heavy chain (Dync1h1), were enriched by immunoprecipitation, suggesting association with keratins, whereas kinesin-1 heavy chain and microtubule-associated protein retinitis pigmentosa 1 (EB1), were not enriched. Dync1h1 abundance was negatively correlated with that of all the septins, suggesting resemblance to a known antagonistic septin-dynein 1 relationship on microtubules.

Conclusion: The cell lines showed remarkable uniformity with respect to the candidates interacting with cytoskeleton. The alpha-spectrin-Baiap2l network may link actin filaments to keratin precursor particles. A smaller interaction network centered on Dync1h1 was negatively correlated with all spectrin-Baiap2l constituents, suggesting that it and its binding partners are excluded from the pedestal-like domain.
High-throughput ultrastructural analysis of macular telangiectasia type 2
Authors: Zucker Charles L. , Bernstein Paul S. , Schalek Richard L. , Lichtman Jeff W. , Dowling John E.
Published: July 2024

Introduction

Macular Telangiectasia type 2 (MacTel), is an uncommon form of late-onset, slowly-progressive macular degeneration. Associated with regional Müller glial cell loss in the retina and the amino acid serine synthesized by Müller cells, the disease is functionally confined to a central retinal region – the MacTel zone.

Methods

We have used high-throughput multi-resolution electron microscopy techniques, optimized for disease analysis, to study the retinas from two women, mother and daughter, aged 79 and 48 years respectively, suffering from MacTel.

Results

In both eyes, the principal observations made were changes specific to mitochondrial structure both outside and within the MacTel zone in all retinal cell types, with the exception of those in the retinal pigment epithelium (RPE). The lesion areas, which are a hallmark of MacTel, extend from Bruch’s membrane and the choriocapillaris, through all depths of the retina, and include cells from the RPE, retinal vascular elements, and extensive hypertrophic basement membrane material. Where the Müller glial cells are lost, we have identified a significant population of microglial cells, exclusively within the Henle fiber layer, which appear to ensheathe the Henle fibers, similar to that seen normally by Müller cells.

Discussion

Since Müller cells synthesize retinal serine, whereas retinal neurons do not, we propose that serine deficiency, required for normal mitochondrial function, may relate to mitochondrial changes that underlie the development of MacTel. With mitochondrial changes occurring retina-wide, the question remains as to why the Müller cells are uniquely susceptible within the MacTel zone.
Autonomic modulation by SGLT2i or DPP4i in patients with diabetes favors cardiovascular outcomes as revealed by skin sympathetic nerve activity
Authors: Chen Jien-Jiun , Lin Chen , Lo Men-Tzung , Lin Lian-Yu , Chang Hsiang-Chih , Liu Geng-Chi
Published: July 2024

Background

Sodium-glucose cotransporter 2 inhibitors (SGLT2i) and dipeptidyl peptidase-4 inhibitors (DPP4i) are important second-line treatments for patients with type 2 diabetes mellitus (T2DM). Patients taking SGLT2i have favorable cardiovascular outcomes via various mechanisms, including autonomic nervous system (ANS) modulation. This study aimed to use neuro-electrocardiography (neuECG) to test the effects of SGLT2i or DPP4i on the ANS.

Methods

Patients with T2DM, who did not reach target hemoglobin (Hb)A1C levels despite metformin treatment, were enrolled. SGLT2i or DPP4i were prescribed randomly unless a compelling indication was present. NeuECG and heart rate were recorded for 10 min before and after a 3-month treatment. The patients were treated according to standard practice and the obtained data for skin sympathetic nerve activity (SKNA) and ANS entropy were analyzed offline.

Results

We enrolled 96 patients, of which 49 received SGLT2i and 47 received DPP4i. The baseline parameters were similar between the groups. No adverse event was seen during the study period. In the burst analysis of SKNA at baseline, all parameters were similar. After the 3-month treatment, the firing frequency was higher in SGLT2i group (0.104 ± 0.045 vs 0.083 ± 0.033 burst/min, p < 0.05), with increased long firing duration (7.34 ± 3.66 vs 5.906 ± 2.921, p < 0.05) in 3-s aSKNA scale; the other parameters did not show any significant change. By symbolic entropy, the most complex patterns (Rank 3) were found to be significantly higher in SGLT2i-treated patients than in DDP4i-treated group (0.084 ± 0.028 vs 0.07 ± 0.024, p = 0.01) and the direction of change in Rank 3, after SGLT2i treatment, was opposite to that observed in the DDP4i group (0.012 ± 0.036 vs. −0.005 ± 0.037, p = 0.024). Our findings demonstrated the favorable autonomic modulation by SGLTi and the detrimental effects of DPP4i on ANS.

Conclusion

We demonstrated the autonomic modulation by SGLTi and DPP4i using SKNA in patients with DM, which might provide insights into the favorable outcomes of SGLT2i. Furthermore, we refined the analytical methods of neuECG, which uses SKNA to evaluate autonomic function.
Polyphyly of Boehmeria (Urticaceae) congruent with plastome structural variation
Authors: Zhan Min , Xue Ling , Zhou Jian-Jun , Zhang Qiang , Qin Xin-Mei , Liao Xiao-Wen , Wu Lei , Monro Alexander K. , Fu Long-Fei
Published: July 2024

Boehmeria is a taxonomically challenging group within the nettle family (Urticaceae). The polyphyly of the genus has been proposed by previous studies with respect to five genera (Debregeasia, Cypholophus, Sarcochlamys, Archiboehmeria, and Astrothalamus). Extensive homoplasy of morphological characters has made generic delimitation problematic. Previous studies in other plant groups suggest that plastome structural variations have the potential to provide characters useful in reconstructing evolutionary relationships. We aimed to test this across Boehmeria and its allied genera by mapping plastome structural variations onto a resolved strongly supported phylogeny. In doing so, we expanded the sampling of the plastome to include Cypholophus, Sarcochlamys, Archiboehmeria, and Astrothalamus for the first time. The results of our phylogenomic analyses provide strong support for Sarcochlamys as being more closely related to Leucosyke puya than to Boehmeria and for the clustering of Boehmeria s.l. into four subclades. The sizes of the plastomes in Boehmeria s.l. ranged from 142,627 bp to 170,958 bp. The plastomes recovered a typical quadripartite structure comprising 127~146 genes. We observe several obvious structural variations across the taxa such as gene loss and multiple gene duplication, inverted repeat (IR) contraction and wide expansions, and inversions. Moreover, we recover a trend for these variations that the early clades were relatively conserved in evolution, whereas the later diverging clades were variable. We propose that the structural variations documented may be linked to the adaptation of Boehmeria s.l. to a wide range of habitats, from moist broadleaf forests in Asia to xeric shrublands and deserts in Africa. This study confirms that variation in plastome gene loss/duplication, IR contraction/expansion, and inversions can provide evidence useful for the reconstruction of evolutionary relationships.
A critical role of a plant-specific TFIIB-related protein, BRP1, in salicylic acid-mediated immune response
Authors: Xu Binjie , Fan Baofang , Chen Zhixiang
Published: July 2024

An integral part of plant immunity is transcription reprogramming by concerted action of specific transcription factors that activate or repress genes through recruitment or release of RNA polymerase II (Pol II). Pol II is assembled into Pol II holoenzyme at the promoters through association with a group of general transcription factors including transcription factor IIB (TFIIB) to activate transcription. Unlike other eukaryotic organisms, plants have a large family of TFIIB-related proteins with 15 members in Arabidopsis including several plant-specific TFIIB-related proteins (BRPs). Molecular genetic analysis has revealed important roles of some BRPs in plant reproductive processes. In this study, we report that Arabidopsis knockout mutants for BRP1, the founding member of the BRP protein family, were normal in growth and development, but were hypersusceptible to the bacterial pathogen Psuedomonas syringae. The enhanced susceptibility of the brp1 mutants was associated with reduced expression of salicylic acid (SA) biosynthetic gene ISOCHORISMATE SYNTHASE 1 (ICS1) and SA-responsive PATHOGENESIS-RELATED (PR) genes. Pathogen-induced SA accumulation was reduced in the brp1 mutants and exogenous SA rescued the brp1 mutants for resistance to the bacterial pathogen. In uninfected plants, BRP1 was primarily associated with the plastids but pathogen infection induced its accumulation in the nucleus. BRP1 acted as a transcription activator in plant cells and binded to the promoter of ICS1. These results collectively indicate that BRP1 is a functionally specialized transcription factor that increasingly accumulates in the nucleus in response to pathogen infection to promote defense gene expression.
Suicidal ideation in medical students of Hebei province: prevalence and associated factors
Authors: Huang Fanfan , Lu Wenting , Zhao Xiaochuan , Li Na , Zhao Tianyu , Guo Shijie , Liu Bufan , Ren Ruojia , Yang Li , Wang Lan , Gao Yuanyuan , Wang Ran , Song Mei , Lin Quan , Xu Yuehang , Jin Na , Liu Kuaikuai , Bai Meina , Wang Xueyi
Published: July 2024

Objectives

This study investigated the prevalence of suicidal ideation (SI) among Chinese medical students and its associated risk factors.

Methods

A total of 6643 medical students (2383 males/4260 females) were recruited from a medical college in Hebei Province, China. Demographic data were collected via a self-administered questionnaire. The Childhood Trauma Questionnaire Short Form (CTQ-SF) was used to evaluate childhood maltreatment (CM), and the Adolescent Self-Rating Life Events Checklist (ASLEC) was used to evaluate the stressful life events. Suicidal ideation was assessed using the Beck Scale for Suicide Ideation (BSSI). Univariate and multivariate logistic regression models were used to analyze the factors affecting SI.

Results

The prevalence of SI in medical students was 11.5% (763/6643). Multivariate logistic regression analysis revealed that SI was significantly associated with younger age, a female sex, being lovelorn, being introverted, experiencing CM during childhood, and experiencing stressful life events within the past 12 months. Of the five subtypes of CM, emotional abuse may have the strongest effect on SI (OR=2.76, 95% CI: 1.72–4.42). The joint effects of CM and stressful life events were significantly associated with an increased risk of SI (OR=5.39, 95% CI: 4.15–6.98).

Conclusion

The prevalence of SI among medical students is high, and medical students who have experienced CM and stressful life events have a higher tendency towards SI. Screening for both CM and stressful life events may be an effective way of identifying individuals at high risk of SI.
Creating, publishing, and spreading processes of health-related contents in internet news sites: evaluation of the opinions of actors in health communication
Authors: Öntaş Eray , Bahar-Özvarış Şevkat , Şimşek Burcu
Published: July 2024

Introduction

The accuracy and reliability of health information disseminated through news is crucial, as it directly impacts both individual and societal health outcomes. This study aims to analyze the publication process of health content in Türkiye and its implications for public health. By examining the perspectives of various health communication stakeholders, the study seeks to identify existing issues and propose potential solutions.

Methods

The research uses a mixed-methods approach, including baseline content analysis of 846 news by 133 criteria, quantitative research with 78 participants encompassing bureaucrats, academics, journalists, and health association members, and 15 in-depth interviews for comprehensive insights.

Results

The content analysis indicated that 23.2% of the analyzed news articles lacked credible sources, while 63% did not mention the author’s name. A striking 96.2% of respondents stated that inaccurate health news poses a risk to public health, emphasizing the urgent need for standardized reporting practices. The majority (90.9%) pinpointed the media as the primary catalysts for infodemic spread, with 93.5% citing gatekeepers as barriers to accurate information. Eroding trust in media, fueled by unethical practices, harms both media credibility and effective public health interventions.

Discussion

The study underscores the necessity for a collaborative approach among public institutions, academia, and media, focusing on responsibility, regulation, and sanctions against the infodemic. The research advocates for a balanced approach that prioritizes health rights and press freedom within a stakeholder-driven framework, highlighting that legislation alone cannot fully enhance the digital information ecosystem.
Virtual-reality exposure treatment for first responder PTSD: a pilot investigation
Authors: Beidel Deborah C. , Bowers Clint A. , Newins Amie. R. , Seaver Christine , Rozek David , McDaniel Carole , Horning Daniel R.
Published: July 2024

Introduction: There is increasing recognition that first responders’ exposure to multiple traumatic events puts them at risk for the development of emotional distress, including depression, substance misuse, posttraumatic stress disorder (PTSD), and suicide. Despite this increased risk and higher prevalence of PTSD and other trauma-related disorders, there are few data on treatment for first responders.

Method: This investigation describes the outcome of an intensive outpatient program (IOP), initially developed for military trauma, as applied to the treatment of first responders with PTSD. This treatment is unique in that it utilizes virtual reality to enhance the immersion into the exposure scene. Furthermore, we assessed the utility of both fixed-length and unbounded-length exposure sessions.

Results: The results indicate that the intervention was feasible to implement and did not produce iatrogenic effects. Participants reported significantly reduced symptoms of PTSD and related emotional distress, reduced disability, and improved daily functioning. These positive outcomes were maintained at 3-month follow-up. There was no difference in outcomes between the fixed- and unbounded-length sessions.

Discussion: These results suggest that an efficacious, short-term intervention incorporating virtual reality has the potential to quickly return first responders to their profession.
Analiza konwersacyjna jako metoda badań logopedycznych w zakresie bilingwizmu polsko-obcego
Authors: Młyński Rafał , Majewska-Tworek Anna
Published: July 2024

The article concerns conversation analysis (CA) as a method derived from sociology, and also used and developed in psychology, linguistics, and speech and language therapy. The article aims at presenting the potential of the method in describing various types of verbal interactions. The text presents the use of CA to explore the dialogic abilities of bilingual children. The authors study code switching (CS) patterns occurring in bilingual children’s speech, the features of these patterns, and conversational strategies that appear in interviews with children. It was found that CS is closely linked to lexical deficits in Polish. The features of conversational negotiation were noticed when using English. Moreover, due to deficiencies in the Polish linguistic repertoire, the speakers used repairs, and the statements of the interlocutors would often overlap.
Obłęd czterech wieszczów– leksemy obłęd i obłąkanie oraz słowa powiązane słowotwórczo w tekstach Adama Mickiewicza, Juliusza Słowackiego, Zygmunta Krasińskiego i Cypriana Norwida
Authors: Pawelec Radosław
Published: July 2024

In the history of the Polish language, the words obłęd (‘madness’) and obłąkanie (‘insanity’) are etymologically associated with wandering. In the 19th century, these meanings were still alive; at the same time, meanings related to the mental sphere were also forming. In the literature of Romanticism, one can see elements that correspond to the content of the word obłęd used as a term in modern psychiatry. These include, first of all, the structure of delusions that accompany a compact, integrated personality. This is how we can interpret, for instance, the usage of the word in Mickiewicz’s translation of The Dream (the same is true, of course, also for Byron’s original), as well as in Czarne kwiaty by Norwid. Among the noteworthy linguistic facts associated with the studied words in the 19th century is their cognitive ambivalence: on the one hand, madness was associated with special cognitive abilities, on the other, stigmatization of the mentally ill was apparent. Finally, the progressive (also in the Modernist period) increase in the number of uses of these words can be observed, which is likely due to the growing interest in the human psyche in art and its study in medicine.
Lester: Rotoscope Animation through Video Object Segmentation and Tracking
Authors: Tous Ruben
Published: July 2024

This article introduces Lester, a novel method to automatically synthesize retro-style 2D animations from videos. The method approaches the challenge mainly as an object segmentation and tracking problem. Video frames are processed with the Segment Anything Model (SAM) and the resulting masks are tracked through subsequent frames with DeAOT, a method of hierarchical propagation for semi-supervised video object segmentation. The geometry of the masks’ contours is simplified with the Douglas–Peucker algorithm. Finally, facial traits, pixelation and a basic rim light effect can be optionally added. The results show that the method exhibits an excellent temporal consistency and can correctly process videos with different poses and appearances, dynamic shots, partial shots and diverse backgrounds. The proposed method provides a more simple and deterministic approach than diffusion models based video-to-video translation pipelines, which suffer from temporal consistency problems and do not cope well with pixelated and schematic outputs. The method is also more feasible than techniques based on 3D human pose estimation, which require custom handcrafted 3D models and are very limited with respect to the type of scenes they can process.
Efficient Detection of Malicious Traffic Using a Decision Tree-Based Proximal Policy Optimisation Algorithm: A Deep Reinforcement Learning Malicious Traffic Detection Model Incorporating Entropy
Authors: Zhao Yuntao , Ma Deao , Liu Wei
Published: July 2024

With the popularity of the Internet and the increase in the level of information technology, cyber attacks have become an increasingly serious problem. They pose a great threat to the security of individuals, enterprises, and the state. This has made network intrusion detection technology critically important. In this paper, a malicious traffic detection model is constructed based on a decision tree classifier of entropy and a proximal policy optimisation algorithm (PPO) of deep reinforcement learning. Firstly, the decision tree idea in machine learning is used to make a preliminary classification judgement on the dataset based on the information entropy. The importance score of each feature in the classification work is calculated and the features with lower contributions are removed. Then, it is handed over to the PPO algorithm model for detection. An entropy regularity term is introduced in the process of the PPO algorithm update. Finally, the deep reinforcement learning algorithm is used to continuously train and update the parameters during the detection process, and finally, the detection model with higher accuracy is obtained. Experiments show that the binary classification accuracy of the malicious traffic detection model based on the deep reinforcement learning PPO algorithm can reach 99.17% under the CIC-IDS2017 dataset used in this paper.