GENETIC ANALYSIS OF THE TYPES OF DEVELOPMENT OF MUTANT LINES FROM COMMON WHEAT VARIETIES
Authors:   2nd year Master's student of al-Farabi Kazakh national University, Sarybay N. ,   sarybay_nazerke1@live.kaznu.kz, https://orcid.org/0000-0002-4678-2533, Chunetova Zh. Zh. ,   Ph. D., associate Professor of the Department of genetics and molecular biology, al-Farabi Kazakh National University; Zhanar.Chunetova@kaznu.kz, https://orcid.org/0000-0002-1150-9668, Iskakova D. M. ,   graduate Student of 2 course of KazNU. Al-Farabi; mailto:Dina.iskakova.98@list.ru, https://orcid.org/0000-0001-7593-2724, Zhumabaeva B.A. ,   Ph. D.,associate Professor of the Department of genetics and molecular biology, al-Farabi Kazakh National University; Beibytgul.Zhumabaeva@kaznu.kz, https://orcid.org/0000-0001-7946-5553, Argynbaev Sh. ,   lecturer in genetics and molecular biology, KazNU al-Farabi; shynaryrgynbaeva@gmail.com, https://orcid.org/0000-0003-0230-0181, Altybaeva N.A. ,   lecturer in genetics and molecular biology, KazNU al-Farabi; Nazgul.Altybaeva@kaznu.kz, https://orcid.org/0000-0002-6373-4699, Ertayeva B. A. ,   teacher of the Department of genetics and molecular biology, al-Farabi Kazakh National University; Bybynur.Ertaeva@kaznu.kz, https://orcid.org/0000-0003-1353-6190
Published: December 2020

Among cereals, a special place is occupied by wheat as the main food of humanity and in improving the country's economy. The main task of breeding is to produce valuable wheat varieties that are stable to the unfavorable external environment of various natural climatic zones of Kazakhstan, consisting of a combination of valuable features. The prospects for the use of mutants in the process of hybridization are reflected in the achievements of world and domestic selection. The effect of various solutions of chemical compounds on the plant was observed from the first days during the growth of wheat sprouts. The growth of the first sprout of seeds treated with a certain solution of cadmium chloride and zinc chloride - 0.01%, the activity of cell division and structural disorders of chromosomes were manifested by variability in the varieties Kazakhstani3, Shagala, Zhenis and Lutescens 32. When treating dry wheat grain with a concentration of 0.1% cadmium chloride, it brought the growth of the Sprout to a lethal state, 0.01% inhibited the growth of the Sprout for 4 days, and in a 0.001% solution, the growth rate of the control grains was the same. On the contrary, a 0.1% vertical solution of zinc chloride inhibited the growth of the Sprout for 1 Week, 0.01% for three days. Among the studied concentrations, a 0.01% solution of cadmium chloride inhibited the growth of sprouts, causing chromosomal aberrations and morphological variability in cell division. Therefore, a concentration of 0.01% of cadmium salt was obtained as an optimal concentration to expand the limits of variability in wheat. In this regard, in our study, it was found that a 0.01% solution of cadmium chloride is an effective concentration that expands the limits of variability in wheat. The effect of this amount of solution leads to morphological changes in the plant (Binding of the stem, elongation of the earlobe, increase in the number and weight of grains in the headlobe, increase in the weight of 1000 grains, etc.), and changes in the characteristics of the M1 - M4 offspring are constantly inherited. Mutant lines L1, L2 and L3 differ in valuable breeding characteristics under the influence of a 0.01 percent solution of cadmium salt were obtained for the Kazakhstanskaya 3 and Shagala varieties. A genetic analysis of the type of development of these altered lines was carried out, as a result of which a long (16 cm) dense ear (0.80) changed from the Gull variety to a prismatic plant of the control variety (0.50), as a result of hybridization, its summer type (from the vrn gene) to the autumn type (Vrn gene). As a result of the study, Kazakhstanskaya 3, Kazakhstanskaya mutant 3 varieties contain the genes Vrn 1, Vrn 3. Well, it was found that Shagala varieties consist of the genes Vrnl, Vrn2. Varieties containing the genes Vrn1, Vrn3 show rapid maturation. However, they differed from each other in the period of intoxication.