bluuebunny/crossref_metadata_2025 huggingface dataset:
Efektivitas Aplikasi Ogan Opian terhadap Pelayanan Publik di Kabupaten Purwakarta
Authors: Syahbani Azizah Nur , Nuraini Siti
Published: September 2024

Kabupaten Purwakarta terus mengembangkan sistem - sistem e-government sampai ke tingkat desa. Salah satu sistem yang saat ini tengah dikembangkan oleh pemerintah Kabupaten Purwakarta ialah Ogan Lopian. meski kota kecil dengan jumlah penduduk kurang dari satu juta jiwa, Kabupaten Purwakarta dalam kurang dari satu dekade telah bertransformasi menjadi kota yang memanfaatkan akses informasi dan teknologi (IT) dalam berbagai aktivitasnya. Hipotesis yang akan digunakan dalam penelitian ini adalah dengan penerapan E-Government maka akan meningkatkan pelayanan terhadap publik khususnya masyarakat Purwakarta. Sedangkan tujuan dari penelitian yang akan dilakukan ini adalah: untuk mengetahui tingkat efektifitas aplikasi Ogan Lopian terhadap penerapan e-Government di kabupaten Purwakarta dalam pelayanan publik. Penelitian yang dipakai dalam penelitian ini adalah kualitatif dengan menggunakan metode deskriptif. Penelitian ini ada dua variabel yang digunakan yaitu Penerapan Electronic Government (X) dan pelayanan publik (Y). Pengumpulan data dengan menggunakan data sekunder yaitu data dari buku, jurnal, artikel dan peraturan undang – undang. Sedangkan teknik yang digunakan yaitu analisis deskritif. Hasil penelitian ini ialah bahwa penggunaan aplikasi Ogan Lopian terhadap penerapan e-government dapat meningkatkan pelayanan publik di Kabupaten Purwakarta, pemerintah Kabupaten Purwakarta telah mengembangkan e-government dengan sangat baik. Sehingga masyarakat Kabupaten Purwakarta dapat memanfaatkan e-government dengan sangat baik.
Causes of high diatom productivity in Neogene Lake of the Southern Primorye
Authors: Avramenko Aleksandra S. , Pushkar Vladimir S.
Published: September 2024

The fossil diatoms were studied from Neogene siliceous organogenic deposits of Primorye. The taxonomic composition of the diatom flora, to evaluate its qualitative (ecological) and quantitative characteristics were established. Based on analysis of the fossil microfossils features to established the conditions for the formation of rocks and the causes that determined the high productivity of diatoms. The probable causes could be: the development of a dense lake-river network; a long growing season under the conditions of a monsoon climate with mild winters that had formed by that time; active volcanism, the eruption products of which were a source of substances necessary for the maintaining vital activity of diatoms and formation of their valves.
Profit over Public Good: The Impact of Investment Firm Ownership on Local News and Political Behavior
Authors: Ewing Sean
Published: September 2024

Local newspapers play an important role in informing the public and holding elected officials accountable, yet the rise of newspaper owners that deal primarily in investments and not media has raised questions about the commitment of newsrooms to reporting on local politics. I document 856 instances of ownership changes among daily newspapers between 2004-2020, with just under half involving a purchase by an owner who primarily deals in investments. I find that a newspaper's acquisition by an investment-owner leads to more stories about national politics and fewer stories about local politics relative to acquisitions by other types of owners. I also find that a transition to investment ownership leads to reductions in both citizen knowledge and voter turnout, which in turn benefits local incumbents. These findings suggest that the rise of investment ownership is harming local newsrooms and the populations they serve.
Roles of science and research in vocational teacher preparation: A case study from a Norwegian VET teacher programme
Authors: Smeplass Eli , Hylander Lene , Zirkle Chris
Published: September 2024

Bridging vocational skills and scientific principles can pose challenges in vocational education and training (VET) teacher programmes. This study aims to enhance such programmes by exploring the potential of reflective practices among students and educators on the nature of science and research in VET contexts. Engaging VET teacher students with multidisciplinary backgrounds in a learning community, the study explores how to further develop research-based teacher training by ‘nudging’ student cooperation. A pedagogical course designed to appreciate students’ prior experiences aimed at preparing them to reflect upon their own professional development from a scientific perspective. Using five focus group interviews with 19 first-year students, the study identifies and explains four key factors for quality in VET teacher training: relevance of pedagogical theory, the value of a multidisciplinary approach, recognition of students’ VET backgrounds, and the importance of community in learning. The findings emphasise the significance of creating coherence between research, vocational skills, educational theory, and real-world applications to develop quality in VET teacher training. This study has implications for educators and policymakers, underlining the need for a comprehensive approach to research-based VET teacher competence that integrates personal, academic, and professional elements.
Measuring enhanced weathering: inorganic carbon-based approaches may be required to complement cation-based approaches
Authors: Hasemer Heath , Borevitz Justin , Buss Wolfram
Published: September 2024

The removal of atmospheric carbon dioxide (CO2) is now essential to meet net zero goals and limit the impacts of climate change. Enhanced weathering is a method of sequestering CO2 that involves the distribution of finely ground silicate rocks over agricultural land. The weathering of these silicate rocks releases cations into solution which can balance dissolved inorganic carbon, effectively removing CO2 from the atmosphere. Despite being a promising method of carbon dioxide removal (CDR), enhanced weathering has been limited by uncertainty surrounding the measurement of CO2 sequestration. This study compares current measurement approaches that focus on quantifying inorganic carbon and cations within the soil and leachate. Cation-based calculations of CDR were compared to inorganic carbon-based calculations of CDR and soil results were compared to leachate results. The recovery rate of cations in the soil fraction was also tested. Three different ground silicate minerals/rocks – basalt, olivine and wollastonite, were mixed with two different soils and were allowed to weather over 16 weeks in 320 pots with and without plants under different watering regimes and the application of an acidifying fertiliser. Soil and leachate samples were analysed for cations by ICP-OES and inorganic carbon by direct CO2 analysis after acidification and total alkalinity titration (in leachate only). The results indicate that the soil retains most enhanced weathering products through the cation exchange reactions. CDR estimated by cations is often greater than CDR estimated by inorganic carbon. Measurement approaches to estimate cations are susceptible to incomplete or improper accounting through the under-extraction of cations stored within the soil-exchangeable pool, the activity of non-carbonic acids and CO2 outgassing. Inorganic carbon-based measurements, including direct inorganic carbon and total alkalinity analysis, are also complicated by the potential for CO2 loss through carbonate precipitation and re-equilibration. Therefore, inorganic carbon-based approaches and cation-based approaches should be reconciled to validate the estimation of CDR. The inorganic carbon-based estimation of CDR in leachate should equal the cation-based estimation of CDR in leachate—which will be achieved after quantification or estimation of the natural mechanisms that affect each approach. These findings will support the development of accurate measurement processes for enhanced weathering.
Data-driven multiscale geomechanical modeling of unconventional shale gas reservoirs: a case study of Duvernay Formation, Alberta, West Canadian Basin
Authors: Xiao Yue , Jiang Weidong , Liang Chong
Published: September 2024

The Duvernay Formation in Canada is one of the major oil and gas source formations in the Western Canadian Sedimentary Basin, located at its deepest point. While it demonstrates promising development potential, challenges arise in the urgent need for integration of geology and engineering models, as well as in optimizing sweet spots, particularly as infill wells and pads become central operational objectives for the shale gas field. A lack of the geomechanical understanding of shale gas reservoirs presents a significant obstacle in addressing these challenges. To overcome this, we implemented data acquisition and prepared historical models and profiles, resulting in an extended high-resolution geological and reservoir property model with a fine grid system. Subsequently, a 3D full-field multi-scale geomechanical model was constructed for the main district by integrating seismic data (100 m), geological structures (km), routine logs (m), core data (cm), and borehole imaging (0.25 m), following a well-designed workflow. The predicted fracturability index (brittleness) ranges from 0.6 to 0.78, and a lower horizontal stress difference (STDIFF) is anticipated in the target formation, Upper Duvernay_D, making it a favorable candidate for hydraulic fracturing treatment. Post-analysis of the multi-disciplinary models and various data types provides guidelines for establishing a specific big database, which serves as the foundation for production performance analysis and aggregate sweet spot analysis. Fourteen geological and geomechanical candidate parameters are selected for the subsequent sweet spot analysis. This study highlights the effectiveness of multi-scale geomechanical modeling as a tool for the integration of multi-disciplinary data sources, providing a bridge between geological understanding and future field development decisions. The workflows also offer a data-driven framework for selecting parameters for sweet spot analysis and production dynamic analysis.
Late Ediacaran to Early Cambrian stratigraphic correlation and its geological implications in the northwestern Sichuan Basin: insights from phosphorus, isotopes, and small shelly fossils
Authors: Li Wenzheng , Zhang Jianyong , Zhu Xinjian , Wang Yongxiao , Tian Xingwang , Fu Xiaodong , Jiang Hua , Zhong Yuan , Liu Shugen , Li Rong , Pan Bing , Deng Bin
Published: September 2024

The characteristics of elements, isotopes, and small shelly fossils were investigated for Late Ediacaran to Early Cambrian stratigraphy division and to discuss their geological implications in the northwestern Sichuan Basin. The results reveal that small shelly fossils can be detected in the high-phosphorous section, with the concentration of phosphorus mainly ranging from 2% to 8%, suggesting that this interval belongs to the Early Cambrian, which is also consistent with the carbon isotopic composition results. In addition, the Early Cambrian is denudated in the Sichuan Basin due to tectonic movement, and the characteristics of some isotopes and small shell fossils are different from those in other basins. It can be proposed that P content can support the recognition of lithological boundaries, and the high phosphorus content can be used as a reference to identify the top and bottom boundaries of the Maidiping Formation in the study area. According to the elemental compositions in the Ediacaran Dengying Formation, the variations in Si, Al, Fe, and K contents are similar in the platform area and rift area, suggesting that the third and fourth member of the Dengying Formation are also developed in the Deyang–Anyue Rift. The results suggest that both the Deng-4 member and Maidiping Formation feature contemporaneous deposition of different facies in the northwestern Sichuan Basin. The strata consist of shale intercalated with thin carbonate rock deposits in the Deyang–Anyue Rift, while carbonate rock deposits in the platform. The Deyang–Anyue Rift expanded gradually in the Late Ediacaran and eventually filled in the Early Cambrian. The data in this study illustrate that elemental compositions, isotopes, and small shelly fossils can be combined to correlate the Late Ediacaran to Early Cambrian strata and provide new evidence for Deyang–Anyue Rift evolution. The results offer some new insights for deep oil and gas exploration in the Sichuan Basin and for the tectonic–depositional–environmental–biological synergistic evolution in the Late Ediacaran to Early Cambrian transition.
Expanding clinical characteristics and genotypic profiling of Yao syndrome in Chinese patients
Authors: Zhang Jingyuan , Huang Xin , Shen Min
Published: September 2024

Objectives

Yao syndrome (YAOS, OMIM# 617321) is a kind of systemic autoinflammatory diseases (SAIDs) linked to the nucleotide-binding oligomerization domain containing 2 (NOD2). Clinical reports of YAOS in China are sparse. Herein, we reported the largest YAOS cohort of Chinese patients to expand the understanding of its phenotype, genotype, and therapeutic responses.

Methods

This study enrolled 15 adult patients diagnosed with YAOS at Peking Union Medical College Hospital from April 2015 to May 2024. Whole-exome sequencing was performed on all patients. Clinical data, genetic variations, and treatment responses were documented and compared with a Caucasian cohort.

Results

The mean age of disease onset was 35 ± 17 years old. The most common clinical manifestations included recurrent high-grade fever (100%), gastrointestinal symptoms (73.3%), arthralgia/arthritis, fatigue, myalgia, and lower extremity swelling (46.7%). All patients exhibited elevated acute-phase reactants during episodes. 12 heterozygous NOD2 variants were identified, with Q902K in 4 patients, R471C in 3, and variants c.-14C>T, A110T, S127L, R311W, A432V, Y514H, R541P, A661P, K818Q, A886V each found in individual patients. 90% of the patients responded well to glucocorticoids, and 55.6% to sulfasalazine. 66.7% of patients who received TNF inhibitors achieved complete resolution of symptoms. Additionally, one patient each responded favorably to canakinumab and tocilizumab. Compared to the Caucasian cohort, our cohort exhibited a more balanced gender ratio and a higher proportion of recurrent fever, proteinuria/hematuria as well as more frequent leukocytosis, elevated acute phase reactants, and anemia. Lower proportions of arthralgia/arthritis, skin rashes, headaches, and sicca-like symptoms were noted in our cohort. Moreover, a higher proportion of patients in our cohort showed a good response to TNF inhibitors.

Conclusion

Chinese patients with YAOS had more pronounced inflammatory manifestations compared to the Caucasian cohort. Variants c.-14C>T, A110T, S127L, A661P, K818Q, A886V, R471C, and A432V were identified as novel NOD2 variants in YAOS. TNF, IL-6, and IL-1 inhibitors are the promising treatment options. These findings expand the clinical spectrum, genetic profile, and treatment efficacy of YAOS, underscoring the need for heightened awareness of this disease in diverse populations.
Local administration of mRNA encoding cytokine cocktail confers potent anti-tumor immunity
Authors: Li Zhigang , Hu Ling , Wang Yi , Liu Qi , Liu Jun , Long Haiyan , Li Qi , Luo Liping , Peng Yucai
Published: September 2024

Immunotherapy using inflammatory cytokines, such as interleukin (IL)-2 and interferon (IFN)-α, has been clinically validated in treating various cancers. However, systemic immunocytokine-based therapies are limited by the short half-life of recombinant proteins and severe dose-limiting toxicities. In this study, we exploited local immunotherapy by intratumoral administration of lipid nanoparticle (LNP)-encapsulated mRNA cocktail encoding cytokines IL-12, IL-7, and IFN-α. The cytokine mRNA cocktail induced tumor regression in multiple syngeneic mouse models and anti-tumor immune memory in one syngeneic mouse model. Additionally, immune checkpoint blockade further enhanced the anti-tumor efficacy of the cytokine mRNAs. Furthermore, human cytokine mRNAs exhibited robust anti-tumor efficacy in humanized mouse tumor models. Mechanistically, cytokine mRNAs induced tumor microenvironment inflammation, characterized by robust T cell infiltration and significant inflammatory cytokine and chemokine production.
Immunotherapy in liver cancer: overcoming the tolerogenic liver microenvironment
Authors: Liu Yanju , Yang Hongyuan , Li Tian , Zhang Na
Published: September 2024

Liver cancer is a major global health concern, ranking among the top causes of cancer-related deaths worldwide. Despite advances in medical research, the prognosis for liver cancer remains poor, largely due to the inherent limitations of current therapies. Traditional treatments like surgery, radiation, and chemotherapy often fail to provide long-term remission and are associated with significant side effects. Immunotherapy has emerged as a promising avenue for cancer treatment, leveraging the body’s immune system to target and destroy cancer cells. However, its application in liver cancer has been limited. One of the primary challenges is the liver’s unique immune microenvironment, which can inhibit the effectiveness of immunotherapeutic agents. This immune microenvironment creates a barrier, leading to drug resistance and reducing the overall efficacy of treatment. Recent studies have focused on understanding the immunological landscape of liver cancer to develop strategies that can overcome these obstacles. By identifying the specific factors within the liver that contribute to immune suppression and drug resistance, researchers aim to enhance the effectiveness of immunotherapy. Prospective strategies include combining immunotherapy with other treatments, using targeted therapies to modulate the immune microenvironment, and developing new agents that can bypass or counteract the inhibitory mechanisms in the liver. These advancements hold promise for improving outcomes in liver cancer treatment.
Feline respiratory disease complex: insights into the role of viral and bacterial co-infections
Authors: Maboni Grazieli , Che Sunoh , Tallmadge Rebecca , De Luca Eliana , Goodman Laura B. , Weese J. Scott , Sanchez Susan
Published: September 2024

Feline respiratory disease complex (FRDC) is a highly prevalent syndrome in cats that often result in fatal outcomes. FRDC etiology is complex, and often, multiple viral and bacterial pathogens are simultaneously associated with disease causation. There is limited information about the role of co-infections in pathogenesis and the current prevalence of pathogens in North America. We aimed to conduct a study using technical advances in molecular diagnosis and statistical modeling analysis to elucidate the occurrence of pathogens and how co-infections affect disease severity. We attained information from three diagnostic laboratories in North America regarding the occurrence of Bordetella bronchiseptica, Chlamydia felis, Mycoplasma, Felid alphaherpesvirus 1 (FeHV-1), feline calicivirus (FCV), and influenza A, along with age, seasonality, sex, and clinical signs. We also evaluated the role of co-infections in disease severity. These pathogens were also investigated in clinically normal cats (control). The most detected pathogens were Mycoplasma, FCV, and FeHV-1. Most pathogens were detected in the control group, highlighting the challenge of interpreting positive testing results. Co-infections of Mycoplasma and FCV, as well as Mycoplasma and FeHV-1, were important predictors of disease severity. Age, sex, and season had a minor impact on pathogen occurrence. This study provides new insights into FRDC and underlines the relevance of diagnostic panels to screen for a range of pathogens, providing knowledge for timely diagnosis and therapeutic interventions.
Breakfast consumption and associated factors and barriers among school-aged children
Authors: Aneley Zelalem , Assaye Hirut , Mekonen Habitamu , Bewket Yenewa , Lake Embet , Fentahun Andualem
Published: September 2024

Background

Breakfast provides the energy and nutrition we need to function at best, both mentally and physically. It is also plausible to propose that children’s general development is impacted when they skip breakfast.

Objective

To assess the prevalence of breakfast consumption and associated factors and predictors among public primary school children in Debremarkos Town, Northwestern Ethiopia, 2020.

Methods

A community-based cross-sectional study was conducted among school-aged children in Debremarkos, Northwest Ethiopia. A multistage random sampling technique was used to select 609 study participants. The children’s parents were interviewed using a pretested, structured questionnaire. For the rest of the analysis, SPSS version 20 was used. Logistic regression analysis was performed to assess the factors associated with breakfast consumption. Statistical significance was determined at a p value <0.05.

Results

Out of the sampled children, 600 respondents participated in the study, for a response rate of 98.5%. The prevalence of regular breakfast consumption among school-aged children was 67.5%. Higher odds of regular breakfast consumption were found among respondents who were females (AOR = 1.72, 95% CI = 0.118–1.773), those who lived in high-income families (AOR = 7.33, 95% CI = 1.036–8.110), and those who had an educated family (AOR = 13.05, 95% CI = 0.019–13.1). However, lower odds of regular breakfast intake were found among respondents aged 9–12 years (AOR = 0.54, 95% CI = 0.369–0.79).

Conclusion

Breakfast is a major health concern for school-aged children in Debremarkos city. Breakfast eating is associated with several factors; the most notable factor is being female, having a high income, and having an uneducated family. Therefore, to prevent children from skipping breakfast, stakeholders must move swiftly.
Structural and functional fetal cardiac imaging using low field (0.55 T) MRI
Authors: Zhang Charlie Yuli , Cleri Michela , Woodgate Tomas , Ramirez Gilliland Paula , Bansal Simi , Aviles Verdera Jordina , Uus Alena U. , Kyriakopoulou Vanessa , St Clair Kamilah , Story Lisa , Hall Megan , Pushparajah Kuberan , Hajnal Joseph V. , Lloyd David , Rutherford Mary A. , Hutter Jana , Payette Kelly
Published: September 2024

Purpose

This study aims to investigate the feasibility of using a commercially available clinical 0.55 T MRI scanner for comprehensive structural and functional fetal cardiac imaging.

Methods

Balanced steady-state free precession (bSSFP) and phase contrast (PC) sequences were optimized by in utero studies consisting of 14 subjects for bSSFP optimization and 9 subjects for PC optimization. The signal-to-noise ratio (SNR) of the optimized sequences were investigated. Flow measurements were performed in three vessels, umbilical vein (UV), descending aorta (DAo), and superior vena cava (SVC) using the PC sequences and retrospective gating. The optimized bSSFP, PC and half-Fourier single shot turbo spin-echo (HASTE) sequences were acquired in a cohort of 21 late gestation-age fetuses (>36 weeks) to demonstrate the feasibility of a fetal cardiac exam at 0.55 T. The HASTE stacks were reconstructed to create an isotropic reconstruction of the fetal thorax, followed by automatic great vessel segmentations. The intra-abdominal UV blood flow measurements acquired with MRI were compared to ultrasound UV free-loop flow measurements.

Results

Using the parameters from 1.5 T as a starting point, the bSSFP sequences were optimized at 0.55 T, resulting in a 1.6-fold SNR increase and improved image contrast compared to starting parameters, as well as good visibility of most cardiac structures as rated by two experienced fetal cardiologists. The PC sequence resulted in increased SNR and reduced scan time, subsequent retrospective gating enabled successful blood flow measurements. The reconstructions and automatic great vessel segmentations showed good quality, with 18/21 segmentations requiring no or minor refinements. Blood flow measurements were within the expected range. A comparison of the UV measurements performed with ultrasound and MRI showed agreement between the two sets of measurements, with better correlation observed at lower flows.

Conclusion

We demonstrated the feasibility of low-field (0.55 T) MRI for fetal cardiac imaging. The reduced SNR at low field strength can be effectively compensated for by strategically optimizing sequence parameters. Major fetal cardiac structures and vessels were consistently visualized, and flow measurements were successfully obtained. The late gestation study demonstrated the robustness and reproducibility at low field strength. MRI performed at 0.55 T is a viable option for fetal cardiac examination.
Modulation of Cd carriers by innovative nanocomposite (Ca+Mg) and Cd-resistance microbes (Bacillus pumilus): a mechanistic approach to enhance growth and yield of rice (Oryza sativa L.)
Authors: Ali Muhammad Azhar , Nafees Muhammad , Waseem Muhammad , Alomrani Sarah Owdah , Al-Ghanim Khalid A. , Alshehri Mohammed Ali , Zheng Hao , Ali Shafaqat , Li Fengmin
Published: September 2024

Cadmium (Cd) is a well-known pollutant in agricultural soil, affecting human health through the food chain. To combat this issue, Ca + Mg (25 mg L−1) nanocomposite and Bacillus pumilus, either alone or combined, were applied to rice plants under Cd (5 mg kg−1, 10 mg kg−1) contamination. In our study, growth and yield traits demonstrated the beneficial influence of Ca + Mg and B. pumilus application in improving rice defense mechanism by reducing Cd stress. Combined Ca + Mg and B. pumilus application increased SPAD (15), total chlorophyll (18), chlorophyll a (11), chlorophyll b (22), and carotenoids (21%) with Cd (10 mg kg−1), compared to the application alone. Combined Ca + Mg and B. pumilus application significantly regulated MDA (15), H2O2 (13), EL (10), and O2•– (24%) in shoots under Cd (10 mg kg−1), compared to the application alone. Cd (10 mg kg−1) increased the POD (22), SOD (21), APX (12), and CAT (13%) in shoots with combined Ca + Mg and B. pumilus application, compared to the application alone. Combined Ca + Mg and B. pumilus application significantly reduced Cd accumulation in roots (22), shoots (13), and grains (20%) under Cd (10 mg kg−1), compared to the application alone. Consequently, the combined application of Ca + Mg and B. pumilus is a sustainable solution to enhance crop production under Cd stress.
Differential gene expression in leaves and roots of Hydrangea serrata treated with aluminium chloride
Authors: Scholpp Anna-Catharina , Schilbert Hanna Marie , Viehöver Prisca , Weisshaar Bernd , Beckstette Michael , Neumann Judith Martha , Bednarz Hanna , Niehaus Karsten
Published: September 2024

Hydrangea serrata, also knowen as the Japanese tea hortensia, is known for its sweet taste and health properties of bevarages produced from this plant. The H. serrata 3,4-dihydroisocoumarins, hydrangenol and phyllodulcin harbour a variety of biological activities and pharmacological properties. Therefore, a detailed understanding of dihydroisocoumarin biosynthesis in H. serrata is of major interest. Their biosynthesis is assumed to be enhanced by elicitors and mediated by polyketide synthases like in cases of phenylpropanoid derived phytoalexins. A de-novo transcriptome assembly of leaves and roots from the aluminium chloride treatment group versus the control group alongside with annotation was generated. Secondary plant metabolites were analysed by LC-MS. It revealed that a terpene synthase and a triterpenoid synthase gene as well as lignin biosynthesis encoding genes were upregulated in roots. Many genes for transporters, glycosyl, and other transferases as well as glycosylases were found to be differentially expressed in both organs. As no differentially expressed polyketide synthase gene homolog was found, the relative leaf and root 3,4-dihydroisocoumarin content was analysed by LC-MS measurement. Although Hydrangea species are known for their aluminium detoxification using phenylpropanoid-derived compounds, the levels of 3,4- dihydroisocoumarins were not enhanced. In this metabolite analysis, an organ- specific accumulation profile of hydrangenol, phyllodulcin, hydrangeic acid and their mono- and di-glycosides was figured out.
Comparative plastome analyses and evolutionary relationships of 25 East Asian species within the medicinal plant genus Scrophularia (Scrophulariaceae)
Authors: Wang Xia , Guo Lei , Ding Lulu , Medina Leopoldo , Wang Ruihong , Li Pan
Published: September 2024

Backgroud

Scrophularia L., a genus of the Scrophulariaceae, is a group of important medicinal plants used for eliminating heat and detoxifying. East Asia has an abundance of potentially medicinal Scrophularia species, and it serves as a secondary diversity center of the genus. However, the genomic resources available for germplasm identification and pharmaceutical exploration of East Asian Scrophularia are insufficient, hindering its commercial and industrial development. Additionally, the interspecific relationships of most East Asian Scrophularia species remain unclear.

Methods

In this study, we sequenced the leaves of 25 East Asian species of the genus Scrophularia, assembled and annotated the complete chloroplast genomes, and subsequently performed comparative and phylogenetic analyses on these genomes.

Results and discussion

The conserved plastome length of these 25 species ranged from 151,582 bp to 153,239 bp, containing a total of 132 coding genes, including 18 duplicated genes and 114 unique genes. Through genome alignment of these 25 species, 38-53 repeated sequences and 7 shared SSRs were identified, along with regions with high nucleotide polymorphism (Pi), which could potentially serve as molecular markers for species identification. The genome structure, gene content, and arrangement showed conservation, while variations were observed in the IR boundary regions and IGS. Phylogenetic inferences based on whole plastomes or on coding sequences (CDS) only yielded congruent results. We categorized the 25 East Asian Scrophularia species into six distinct clades and further explored their interspecies relationships using morphological characteristics, such as flower color, the relative position of stamens and corolla, and plant height. This could lay a genetic basis for future resource development of Scrophularia in East Asia.
The assembly and comparative analysis of the first complete mitogenome of Lindera aggregata
Authors: Shi Yujie , Chen Zhen , Jiang Jingyong , Wu Wenwu , Yu Weifu , Zhang Shumeng , Zeng Wei
Published: September 2024

Lindera aggregata, a member belongs to the genus Lindera of Lauraceae family. Its roots and leaves have been used as traditional Chinese medicine or functional food for thousands of years. However, its mitochondrial genome has not been explored. Our aim is to sequence and assemble the mitogenome of L. aggregata to elucidate the genetic mechanism and evolutionary pathway. The results had shown that the mitogenome was extremely complex and had a unique multi-branched conformation with total size of 912,473 bp. Comprehensive analysis of protein coding genes of 7 related species showed that there were 40 common genes in their mitogenome. Interestingly, positive selection had become an important factor in the evolution of ccmB, ccmFC, rps10, rps11 and rps7 genes. Furthermore, our data highlighted the repeated trend of homologous fragment migrations between chloroplast and mitochondrial organelles, and 38 homologous fragments were identified. Phylogenetic analysis identified a tree that was basically consistent with the phylogeny of Laurales species described in the APG IV system. To sum up, this study will be helpful to the study of population genetics and evolution of Lindera species.
Court-ordered forensic psychiatry treatment in prison: determinants of outcome and risk mitigation
Authors: Weber Kerstin , Morier Sandrine , Menu Christophe , Bertschy Philippe , Herrmann François R. , Giannakopoulos Panteleimon
Published: September 2024

Background

Court-ordered forensic psychiatry treatments (COT) are specifically designed to reduce the risk of violence in mentally disordered offenders. Given their high costs and ethical issues, mental health professionals need admission criteria to be able to select those candidates with optimal benefit. This study analyses offender-related and treatment-related determinants of COT outcome and risk mitigation.

Methods

This two-year longitudinal study assessed the evolution of 117 adult offenders admitted to a specialized medium-security forensic psychiatry clinic. Treatment outcome included court-ordered discharge locations and the Historical Clinical Risk Management (HCR) score evolution. Treatment progress was assessed every six months across five time-points including measures of protective factors, work rehabilitation and security. Outcome determinants included psychiatric diagnosis and type of offence.

Results

Discharge locations are predicted by pre-treatment risk level. Lower HCR scores are associated with discharge into low-security psychiatry wards independently of the psychiatric diagnosis. Risk reduction follows diagnosis-specific and offense-related patterns and reveals that mentally disordered offenders with Cluster B personality disorders or those sentenced for drug crimes are significantly less prone to benefit from COT.

Conclusions

Our findings indicate that criminological characteristics at baseline as well as diagnosis of personality disorders are the main determinants of treatment outcome in our care setting. Inmates with concomitant higher violence risk at baseline and presence of Cluster B personality disorders might benefit the least from court-ordered forensic inpatient psychiatric care in prison.
Examining the audiovisual therapy effects on hospital groups of varying linear canopy landscapes and those with hydrodynamic forces
Authors: Zhang Ping , Cui Yixin , Luo Ke , Zhang Tongyao , Yang Yanbin , Li Jinpeng , Chen Mingze , Chen Hao , He Qianyi , Yu Zheng , Wang Guangyu , Wang Xiaohua , Guo Weiquan , Li Xi , Ma Jun
Published: September 2024

Recent research has highlighted the beneficial effects of urban green spaces on physical and mental health. This study focused on the hospital population and innovatively subdivided the population into four groups: doctors, caregivers, patients and nurses. A total of 96 volunteers participated in this virtual reality experiment to assess the restoration of a linear canopy landscape and a landscape with different levels of hydrodynamics through interactive audiovisual immersion. We utilized pre-research method, brainwave monitoring technique, psychological scales, observation and interviews in this experiment. The research identified five key findings. First, both linear canopy landscapes and those with low to medium hydrodynamic forces significantly enhance physiological and psychological restoration for all groups, with the most substantial physiological benefits observed in doctors and patients, and the greatest psychological relief noted in caregivers. Second, landscapes with medium hydrodynamic forces yield higher restorative effects than those with low forces in hospital settings. Third, green landscapes with medium and low-density canopies prove more conducive to patient recovery compared to those with high-density canopies. Fourth, the inclusion of bird songs does not markedly affect physiological restoration across the hospital groups. Finally, landscapes that incorporate elements of water dynamics, open skies, and lightly foliated canopies draw significant interest from all groups involved. This study advocates for the integration of natural blue and green elements into hospital environments as complementary therapeutic interventions, aiming to alleviate stress and promote health recovery among hospital communities.
Transverse magnetic field effects on diamond quantum sensor for EV battery monitor
Authors: Hatano Yuji , Tanigawa Junya , Nakazono Akimichi , Sekiguchi Takeharu , Kainuma Yuta , Abe Hiroshi , Onoda Shinobu , Ohshima Takeshi , Iwasaki Takayuki , Hatano Mutsuko
Published: September 2024

Key implementation points for achieving full accuracy in simultaneous temperature and magnetic field measurement and linearity when applying diamond quantum sensors to electric vehicle (EV) battery monitors were investigated. Both the static and busbar current magnetic field are required to be aligned to the NV-axis. If misalignment should exist, the resonance frequency midpoint move in the direction opposite to the temperature change under a large busbar current due to the transverse magnetic field effect. Misalignment could be quantified with an accuracy of ±1° by analysing the resonance frequency midpoint change under a current of ±1,000 A. The transverse magnetic field effects compensation estimated from misalignment, confirmed that the resonance frequency midpoint changed consistently with temperature changes. Furthermore, linearity over a wide dynamic range also improved. Moreover, it will contribute to accurate alignment of the two sensors for differential detection to eliminate external noise as common mode. These are expected to expand the application of diamond sensors for high-precision measurement in a wide dynamic range.
Health status of senior netball players, their medication use and attitudes towards doping
Authors: de Abreu Micaela , Nolte Kim , Janse van Rensburg Dina Christa , Swart Xan
Published: September 2024

Limited research exists on the health and injuries of South African senior netball players. Senior netball players may be at greater risk of injuries and chronic disease due to their age. To treat these conditions, they may use prescription and over-the-counter (OTC) medications and, therefore, may be more vulnerable to unintentional doping. The primary aim of this study was to determine the health status, medication use and attitudes towards doping of South African senior netball players. A cross-sectional descriptive design was employed to collect data by means of an online survey. The validated 8-item Performance Enhancement Attitudes Scale (PEAS) was used to gather information on the netball player's attitudes towards doping. Descriptive statistics were used to describe the data using proportions (categorical), means (normally distributed, continuous) and medians (non-normal distributed, continuous). Doping prevalence and accompanying 95% confidence interval were calculated. Sixty senior netball players consented and completed the self-report questionnaire. The prevalence of chronic disease was 11.67%. Asthma and other conditions such as depression and attention-deficit/hyperactivity (ADHD) had the highest prevalence of 3.33%. The prevalence of chronic prescription medication use was 8.33% and 66.67% of the netball players reported receiving prescription injections, medications or utilizing OTC medications for treating injury or illness suffered 1–6 weeks before or during competition. The netball players do not have a lenient attitude towards doping. The prescription and OTC medication use could put this cohort of netball players at risk of unintentional doping. Anti-doping education aimed at senior athletes may be beneficial to reduce the risk of unintentional doping due to prescription and OTC medication use for injury or illness.
Long-Term Manuring Enhanced Compositional Stability of Glomalin-Related Soil Proteins through Arbuscular Mycorrhizal Fungi Regulation
Authors: Yang Hongbo , Cai Zejiang , De Clerck Caroline , Meersmans Jeroen , Colinet Gilles , Zhang Wenju
Published: September 2024

Glomalin-related soil proteins (GRSP) play a crucial role in strengthening soil structure and increasing carbon (C) storage. However, the chemical stability of GRSP and related arbuscular mycorrhizal fungi (AMF) community response to fertilization remains unclear. This study investigated C and nitrogen (N) contents, three-dimensional fluorescence characteristics in GRSP, and AMF properties based on a field experiment that was subjected to 29 years of various fertilizations. The experiment included treatments with no fertilizer (CK), chemical fertilizer (NPK), manure (M), and manure combined with NPK (NPKM) treatments. Results showed that GRSP contained 37–49% C and 6–9% N, respectively. Compared with CK and NPK, the C and N proportions in GRSP significantly increased under M and NPKM. Using the parallel factor model, four fluorescent components of GRSP were identified: one fulvic acid-like component (C2), one tyrosine-like component (C4), and two humic acid-like components (C1, C3). Under M and NPKM, the fluorescent intensity of C2 and C4 decreased, while the humification index (HIX) increased relative to CK and NPK, indicating that organic fertilization could enhance the stability of GRSP. The C and N proportion in GRSP positively associated with soil organic C (SOC), total N (TN), available phosphorus (AP), AMF biomass, and diversity, while C2 and C4 showed negative associations. Structural equation modeling further revealed that manure-induced changes in pH, SOC, TN, and AP increased AMF biomass and diversity, thereby altering GRSP composition and stability. This study provides valuable insights into the compositional traits of GRSP, contributing to sustainable soil management and C sequestration in agroecosystems.
Odor Fences Have No Effect on Wild Boar Movement and Home Range Size
Authors: Faltusová Monika , Ježek Miloš , Ševčík Richard , Silovský Václav , Cukor Jan
Published: September 2024

Wild boars are an opportunistic wildlife species that has successfully colonized the human-modified landscape in Europe. However, the current population boom has negative consequences, which result in a rapid increase in human–wildlife conflicts and disease transmission, including African swine fever (ASF). The increasing frequency of conflicts requires adequate solutions for these issues through various measures. Application of deterrents is a common non-lethal measure whose effects have been insufficiently verified until recently. Thus, this study aims to evaluate the effectiveness of odor fences, often applied as a barrier against wild boar movement. For this purpose, 18 wild boars were marked with GPS collars. After 22 days of initial monitoring, 12 sections of odor fences were installed on their home ranges. The monitored wild boars crossed the area 20.5 ± 9.2 times during the pre-installation period and 19.9 ± 8.4 times after the odor fence installation. Moreover, the average home range varied between 377.9 ± 185.0 ha before and 378.1 ± 142.2 ha after the odor fence installation. Based on GPS telemetry results, we do not support using odor repellent lines for crop protection or for limiting wild boar movement to lessen ASF outbreaks.
Physiological Anti-Müllerian Hormone Concentrations in Male and Female Dogs and Cats before and around Puberty
Authors: Alexander Tanja , Flock Ulrike , Klein Ruth , Reese Sven , Meyer-Lindenberg Andrea , Walter Beate
Published: September 2024

In recent years several studies established the diagnostic value of anti-Müllerian hormone (AMH) in companion animals. However, less is known about physiological AMH concentrations in young individuals highlighting the necessity to apply the diagnostic findings to this group. The aim of this study was to determine the AMH values of healthy male and female dogs between the age of 8 and 48 weeks, tomcats under 8 weeks and up to 48 weeks of age and queens between 2 to 12 weeks of age. In total, 96 blood samples were collected. Anti-Müllerian hormone was measured in all samples and testosterone was measured in the oldest age group of the males in both species. The hormones were analyzed using a human based chemiluminescence immune assay. Overall, AMH concentrations were higher in males than in females (p < 0.001). According to the AMH concentration there was no difference in males, but queens had significant higher AMH concentrations than bitches (p < 0.001). AMH remained high in males up to week 24 and decreased significantly thereafter (tomcats: p = 0.015; male dogs: p = 0.013), which correlated with an increase in testosterone levels for male dogs only. In bitches, AMH remained below the detection limit until the week 16 and slightly increased subsequently. In queens, AMH was detectable from the beginning with a significant increase in the older age group (p = 0.003). Half of the cats in the older age group even approached the chemiluminescence immune assay’s upper limit. The results show that female cats secrete AMH much earlier than female dogs in which AMH secretion begins just shortly before the start of the puberty. In the male animals, the decrease in AMH concentration around puberty was similar in dogs and cats, but a correlation with the increase of testosterone was only observed in dogs. Further research is required to determine the origin of the high AMH concentrations in female kittens and the lack of correlation between testosterone and AMH concentrations in male kittens.
Assessing the Influence of Urine pH on the Efficacy of Ciprofloxacin and Fosfomycin in Immunocompetent and Immunocompromised Murine Models of Escherichia coli and Klebsiella pneumoniae Infection in the Lower Urinary Tract
Authors: Herrera-Espejo Soraya , Carretero-Ledesma Marta , Bahamonde-García Manuel Anselmo , Cordero Elisa , Pachón Jerónimo , Pachón-Ibáñez María Eugenia
Published: September 2024

In vitro studies have suggested that acidic pH may reduce and increase the efficacy of ciprofloxacin and fosfomycin, respectively, when used to treat Escherichia coli and Klebsiella pneumoniae infections. We assessed the effects of acidic, neutral, and alkaline urine pH on the efficacy of optimized ciprofloxacin and fosfomycin dosages in UTI murine model of E. coli and K. pneumoniae. Immunocompetent and immunocompromised mice with adjusted urine pH were inoculated with E. coli and K. pneumoniae strains, and the efficacy was assessed based on the bacterial concentrations in tissues and fluids at 72 h, with respect to untreated controls. At acidic urine pH, both antimicrobials were effective, achieving similar reductions in E. coli concentrations in the kidneys in immunocompetent and immunocompromised mice and in K. pneumoniae in immunocompetent mice. At a neutral urine pH, both therapies reduced the presence of E. coli in the kidneys of immunocompetent mice. However, in immunocompromised mice, antimicrobials were ineffective at treating E. coli infection in the kidneys at a neutral urine pH and showed reduced efficacy against K. pneumoniae at both acidic and neutral urine pH. The results showed no correlation between urine pH and antimicrobial efficacy, suggesting that the reduced effectiveness is associated with the animals’ immunocompetence status.