bluuebunny/crossref_metadata_2025 huggingface dataset:
Advocacy
Authors: Pannick KC David
Published: February 2023

Lord Pannick celebrates advocacy: that controversial legal issues are decided in court after reasoned argument in which the participants refrain (usually) from shouting, personal insults or threats, and the points on each side of the debate are tested for their relevance, their accuracy, and their strength. The book seeks to identify the central characteristics of good and bad advocacy with the aid of examples from courtrooms in the UK and abroad. Lord Pannick also examines the morality of advocacy - that the advocate sets out views to which he does not necessarily subscribe, on behalf of clients for whom she may feel admiration, indifference, or contempt. Lord Pannick seeks to answer the question he is often asked - more by friends than by judges - 'How can you act for such terrible people?'. Finally, he addresses the future of advocacy, arguing it should and will survive pressures for efficiency and technological developments.
Depolarization of intense laser beams by dynamic plasma density gratings
Authors: Wang Y. X. , Weng S. M. , Li P. , Shen Z. C. , Jiang X. Y. , Huang J. , Zhu X. L. , Ma H. H. , Zhang X. B. , Li X. F. , Sheng Z. M. , Zhang J.
Published: February 2023

As a typical plasma-based optical element that can sustain ultra-high light intensity, plasma density gratings driven by intense laser pulses have been extensively studied for wide applications. Here, we show that the plasma density grating driven by two intersecting driver laser pulses is not only nonuniform in space but also varies over time. Consequently, the probe laser pulse that passes through such a dynamic plasma density grating will be depolarized, that is, its polarization becomes spatially and temporally variable. More importantly, the laser depolarization may spontaneously take place for crossed laser beams if their polarization angles are arranged properly. The laser depolarization by a dynamic plasma density grating may find application in mitigating parametric instabilities in laser-driven inertial confinement fusion.
Direct numerical simulation of one-sided forced thermal convection in plane channels
Authors: Pirozzoli Sergio , Modesti Davide
Published: February 2023

We carry out direct numerical simulations (DNS) of turbulent flow and heat transfer in pressure-driven plane channels, by considering cases with heating on both walls, as well as asymmetric heating limited to one of the channel walls. Friction Reynolds numbers up to

${Re}_{\tau } \approx 2000$

are considered, and Prandtl numbers from

${Pr}=0.025$

to

${Pr} = 4$

, the temperature field being regarded as a passive scalar. Whereas cases with symmetric heating show close similarity between the temperature and the streamwise velocity fields, with turbulent structures confined to either half of the channel, in the presence of one-sided heating the temperature field exhibits larger regions with coherent fluctuations extending beyond the channel centreline. Validity of the logarithmic law for the mean temperature is confirmed, as well as universality of the associated von Kármán constant, which we estimate to be

$k_{\theta } = 0.459$

. Deviations from the logarithmic behaviour are much clearer in cases with one-sided heating, which feature a wide outer region with parabolic mean temperature profile. The DNS data are exploited to construct a predictive formula for the heat transfer coefficient as a function of both Reynolds and Prandtl number. We find that the reduction of the thermal efficiency in the one-sided case is approximately

$20\,\%$

at unit Prandtl number; however, it can become much more significant at low Prandtl number.
Experiences with energy drink consumption among Norwegian adolescents
Authors: Kaldenbach Siri , Strand Tor Arne , Holten-Andersen Mads Nicolaj
Published: February 2023

The objective of the present study was to describe adolescents’ habits and experiences with energy drink (ED) consumption and the relation to the amount of ED consumed. We used the national cross-sectional study Ungdata, conducted in 2015–16 in Norway. A total of 15 913 adolescents aged 13–19 years answered questions about ED consumption related to the following topics: reasons for, experiences with, habits and parental attitudes. The sample comprised only adolescents reporting to be ED consumers. We estimated the association between the responses and the average daily consumption of ED in multiple regression models. Those who consumed ED ‘to concentrate’ or ‘to perform better in school’ consumed on average 73⋅1 (CI 65⋅8, 80⋅3) and 112⋅0 (CI 102⋅7, 121⋅2) ml more daily, respectively, than those who did not consume ED for these reasons. Up to 80 % of the adolescents reported that ‘my parents think it is OK that I drink energy drink’, but at the same time almost 50 % reported that ‘my parents say that I shouldn't drink energy drink’. Apart from increased endurance and feeling stronger, both desired and adverse effects of ED consumption were reported. Our findings indicate that the expectation created by the ED companies have great influence on the adolescents’ consumption rate and that parental attitudes towards ED have little to no influence on the adolescents’ consumption rate.
Spatial distribution of stunting and wasting in 6–59 months children in Nepal: analysis using a Bayesian distributional bivariate probit model
Authors: Vatsa Richa , Ghimire Umesh , Sapkota Suman , Subedi Raj Kumar
Published: February 2023

The combined burden of stunting and wasting in children under five years is a serious public health concern. The present study aimed to estimate the joint burden of stunting and wasting among children aged 6–59 months and explore its spatial variation across Nepal. The 2016 Nepal Demographic and Health Survey data was used to study acute and chronic childhood malnutrition. A Bayesian distributional bivariate probit geoadditive model was designed to study the linear association and geographical variation of stunting and wasting among 6–59 months, children. Child-related factors such as low birth weight, fever in the last 2 weeks preceding the survey and fourth or greater birth order were associated with a higher likelihood of stunting. The likelihood of a child being stunted was significantly less in the wealthiest households, having improved toilet facilities, and if mothers were overweight. Children from severely food insecure households were significantly more likely, and children from poorer households were significantly less likely to suffer both acute and chronic malnutrition simultaneously. Results from spatial effect showed that children from Lumbini and Karnali had a higher burden of stunting, and the likelihood that achild would have been wasted was significantly higher in Madhesh and Province 1. Immediate nutritional efforts are vital in low-income and severely food insecure households to lessen the risk of stunting and wasting in under children. Disproportionate geographic variations in stunting and wasting warrant sub-regional-specific nutrition intervention to achieve nutrition targets and reduce the burden of childhood malnutrition across the country.
Biological Differences between Late 19th and Early 20th Century Urban and Rural Residence
Authors: Carson Scott Alan
Published: February 2023

Communities urbanize when the net benefits to urbanization exceed rural areas. Body mass, height, and weight are biological welfare measures that reflect the net difference between calories consumed and calories required for work and to withstand the physical environment. Individuals of African-decent had greater BMIs, heavier weights, and shorter statures. Urban farmers had lower BMIs, shorter statures, and lower weight than rural farmers. Over the late 19th and early 20th centuries, urban and rural BMIs, height, and weight were constant, and rural farmers had greater BMIs, taller statures, and heavier weights than urban farmers and workers in other occupations.
Protein modifications due to homogenisation and heat treatment of cow milk
Authors: Gathercole Jessica L. , Nguyen Hanh T. H. , Harris Paul , Weeks Mike , Reis Mariza G.
Published: February 2023

This research paper aimed to locate protein modifications caused by treatment of milk and determine if the modification locations were consistent. The majority of milk for consumption is homogenised using pressure and heat, and this causes changes in the location of proteins in the milk as well as protein modifications. To investigate these proteomic changes, raw milk was pasteurised (72°C, 15 s), then, to separate the treatment for homogenisation, heated at these different pressures and temperatures: 45°C without no pressure applied, 45°C with 35 MPa, 80°C without pressure applied and 80°C, with 35 MPa. Proteomic analysis was done after separating the milk into three fractions: whey, casein and cream. Protein modifications in each fraction were examined and we found Maillard products as well as oxidation to be of interest. The proteins were also further identified and characterised to compare protein modification sites and differences in proteins present in the cream resulting from homogenisation and/or pasteurisation. This experiment showed that both heat and pressure during homogenisation can cause increases in protein modifications as a result of oxidation or the Maillard reaction. Total cysteine oxidation and total proline oxidation differed between treatments although this was only significantly different for cysteine. It was observed that protein modifications occurred in the same location in the protein sequence rather than in random locations which we highlighted by examining α-S1-casein, lactadherin and β-lactoglobulin.
On some hyperelliptic Hurwitz–Hodge integrals
Authors: LEWAŃSKI DANILO
Published: February 2023

We address Hodge integrals over the hyperelliptic locus. Recently Afandi computed, via localisation techniques, such one-descendant integrals and showed that they are Stirling numbers. We give another proof of the same statement by a very short argument, exploiting Chern classes of spin structures and relations arising from Topological Recursion in the sense of Eynard and Orantin.

These techniques seem also suitable to deal with three orthogonal generalisations: (1) the extension to the r-hyperelliptic locus; (2) the extension to an arbitrary number of non-Weierstrass pairs of points; (3) the extension to multiple descendants.
Sensory processing challenges as a novel link between early caregiving experiences and mental health
Authors: Méndez Leal Adriana S. , Alba Laura A. , Cummings Kaitlin K. , Jung Jiwon , Waizman Yael H. , Guassi Moreira João F. , Saragosa-Harris Natalie M. , Ninova Emilia , Waterman Jill M. , Langley Audra K. , Tottenham Nim , Silvers Jennifer A. , Green Shulamite A.
Published: February 2023

Early caregiving adversity (ECA) is associated with elevated psychological symptomatology. While neurobehavioral ECA research has focused on socioemotional and cognitive development, ECA may also increase risk for “low-level” sensory processing challenges. However, no prior work has compared how diverse ECA exposures differentially relate to sensory processing, or, critically, how this might influence psychological outcomes. We examined sensory processing challenges in 183 8-17-year-old youth with and without histories of institutional (orphanage) or foster caregiving, with a particular focus on sensory over-responsivity (SOR), a pattern of intensified responses to sensory stimuli that may negatively impact mental health. We further tested whether sensory processing challenges are linked to elevated internalizing and externalizing symptoms common in ECA-exposed youth. Relative to nonadopted comparison youth, both groups of ECA-exposed youth had elevated sensory processing challenges, including SOR, and also had heightened internalizing and externalizing symptoms. Additionally, we found significant indirect effects of ECA on internalizing and externalizing symptoms through both general sensory processing challenges and SOR, covarying for age and sex assigned at birth. These findings suggest multiple forms of ECA confer risk for sensory processing challenges that may contribute to mental health outcomes, and motivate continuing examination of these symptoms, with possible long-term implications for screening and treatment following ECA.
Locally available compost application in organic farms: 2-year effect on biological soil properties
Authors: Assirelli A. , Fornasier F. , Caputo F. , Manici L. M.
Published: February 2023

Composting technologies have progressed parallel to the growing interest in recycling organic waste over recent decades, whilst in-field compost application requires technical improvement and more experience in order to optimize their effect according to the agro-environment and the type of crop which follow their incorporation into the soil. In response to compost application, biological soil features were assessed in field by adopting precision agricultural machinery and by limiting soil incorporation to a depth of 15 cm. A 2-year trial was carried out on two sites in the East Po valley (Northern Italy), an agricultural district which, in 2000, was classified as being on the verge of desertification, and where efforts to counteract soil organic matter decline have been underway for some decades. A green-waste compost produced in accordance with current national directives was applied in autumn 2019 and 2020 to two organic fields using precision farming machinery for compost spreading and conventional harrows for incorporation. Fields were divided into two large plots to compare the effect of compost treatment to an untreated control and were managed according to organic farming practices. Seven months after application, microbial biomass, assessed in terms of DNA, and 17 enzymatic activities were estimated by sampling root-explored soil at the vegetative stage of different seed crops for organic horticulture. A significant overall increase of biological soil activity was detected after the second application. The qualitative response varied slightly between the two sites: a higher impact of microbial biomass was observed in the site that was poorer in soil organic matter; whilst in the other, an increase of phosphatase activities contributed more to the general increase of biological activity. Findings show that, in those agricultural soils, an agronomic advantage from compost can be obtained only after repeated applications; furthermore, precision farming technologies facilitate compost application even in small, specialized farms such as those which hosted this trial.
Seismicity Properties of the Chain Transform Fault Inferred Using Data From the PI‐LAB Experiment
Authors: Leptokaropoulos K. , Rychert C. A. , Harmon N. , Kendall J. M.
Published: February 2023

Oceanic transform faults are intriguing in that they do not produce earthquakes as large as might be expected given their dimensions. We use 1‐year of local seismicity (370 events aboveMC = 2.3) recorded on an array of ocean bottom seismometers (OBSs) and geophysical data to study the seismotectonic properties of the Chain transform, located in the equatorial Mid‐Atlantic. We extend our analysis back in time by considering stronger earthquakes (MW ≥ 5.0) from global catalogs. We divide Chain into three areas (east, central, and west) based on historical event distribution, morphology, and multidimensional OBS seismicity cluster analysis. Seismic activity recorded by the OBS is the highest at the eastern area of Chain where there is a lozenge‐shaped topographic high, a negative rMBA gravity anomaly, and only a few historicalMW ≥ 5.5 events. OBS seismicity rates are lower in the western and central areas. However, these areas accommodate the majority of seismic moment release, as inferred from both OBS and historical data. Higherb‐values are significantly correlated with lower rMBA and with shallower bathymetry, potentially related to thickened crust. Our results suggest high lateral heterogeneity along Chain. Patches with moderate to low OBS seismicity rates that occasionally hostMW ≥ 6.0 earthquakes are interrupted by segments with abundant OBS activity but few historical events with 5.5 ≤ MW < 6.0. This segmentation is possibly due to variable fluid circulation and alteration, which may also change in time.
Estimation of Moho Depth Beneath Southern Indian Shield by Inverting Gravity Anomalies Constrained by Seismic Data
Authors: Roy Arka , Prasad Muthyala , B Padma Rao , Tomson J. K.
Published: February 2023

This study presents a high‐resolution 3D Moho structure beneath southern India and its surrounding regions from observed gravity anomalies. The global gravity disturbance model (XGM2019e) with a grid resolution of 0.1° is considered for this study. The extended Bott's inversion algorithm and Gauss‐Fast Fourier Transform based forward model are adopted to invert for the Moho undulations beneath the Indian peninsula. The inversion algorithm is tested for a synthetic model having a predefined density contrast and mean Moho depth. The robustness of this inversion algorithm is further tested for noise‐incorporated gravity data. The control points are required for estimating two hyper‐parameters, viz. density contrast, and reference depth, which play a crucial role in the precise estimation of Moho depth. In real case study, the inverted Moho depth of Southern India and its surrounding regions by seismic constraint receiver function‐driven control points show a very complex architecture of Moho topography. The observed average crustal thickness in the study region is 35.35 km, corroborating with the previously reported Moho depths. The maximum crustal thickness is 53.04 km beneath the southern part of Archean Western Dharwar Craton and west of Salem block, around 44–47 km Moho depth is observed at the south of Salem block into Madurai block till Achankovil Shear Zone, which suggests the possible continuation of the Achaean crust of Palghat‐Cauvery Shear Zone System. The lowest crustal thickness values are observed along the eastern margin of the Cuddapah basin, which overlaps with the Proterozoic Krishna basin of the Eastern Ghats Mobile belt.
Hydrostatic and Non‐Hydrostatic Baroclinic Instability in the Dynamical Core of the DOE Global Climate Model
Authors: Sun Xiaoming , Nadiga Balasubramanya T. , Taylor Mark A. , Puthan Pranav S. , Urrego‐Blanco Jorge R.
Published: February 2023

The dynamical core of the Department of Energy global climate model is used to understand the role of non‐hydrostatic dynamics in the simulation of dry and moist baroclinic waves. To reduce computational cost, the Diabatic Acceleration and REscaling approach is adopted. Scale analysis and numerical simulations suggest that the model solution is not distorted by the change of spherical metric terms due to the change of Earth radius, neither are the inter‐scale interactions strongly altered as indicated by the analysis of spectral flux. Compared with hydrostatic simulations, the onset of baroclinic instability is delayed under non‐hydrostatic dynamics as the associated weaker vertical motions tend to increase the critical wavelength and narrow the range of waves that can be baroclinically unstable. During the development of baroclinic waves, non‐hydrostatic dynamics tends to induce vertical motions in the upper troposphere, accelerating the eastward propagation of upper‐level ridges through their impact on local vorticity tendency. These processes reduce the westward tilt of the vertical ridge axes and suppress the conversion of mean flow available potential energy to eddy kinetic energy, leading to weaker baroclinic eddies than in hydrostatic simulations. The contrasts between hydrostatic and non‐hydrostatic settings hold in supplemental experiments that vary the background flow Rossby number, the amount of water vapor content and vertical resolution. We also find that mesoscale and smaller‐scale activities can be considerably under‐represented when the vertical resolution is limited to that typically used in global climate models.
Logjam Characteristics as Drivers of Transient Storage in Headwater Streams
Authors: Marshall A. , Zhang X. , Sawyer A. H. , Wohl E. , Singha K.
Published: February 2023

Logjams in a stream create backwater conditions and locally force water to flow through the streambed, creating zones of transient storage within the surface and subsurface of a stream. We investigate the relative importance of logjam distribution density, logjam permeability, and discharge on transient storage in a simplified experimental channel. We use physical flume experiments in which we inject a salt tracer, monitor fluid conductivity breakthrough curves in surface water, and determine breakthrough‐curve skewness to characterize transient storage. We then develop a companion numerical model in HydroGeoSphere to reveal flow paths through the subsurface (or hyporheic zone) that contribute to some of the longest transient‐storage timescales. In both the flume experiments and numerical simulations, we observe backwater formation and an increase in hyporheic exchange at logjams. Observed complexities in transient storage behavior depend largely on surface water flow in the backwater zone. As expected, multiple successive logjams provide more pervasive hyporheic exchange by distributing the head drop at each jam, leading to distributed but shallow flow paths. Decreasing the permeability of a logjam or increasing the discharge both facilitate greater surface water storage and volumetric rate of hyporheic exchange. Understanding how logjam characteristics affect solute transport through both the channel and hyporheic zone has important management implications for rivers in forested, or historically forested, environments.
New Discoveries in Old Records
Authors: Micalizio Caryl-Sue
Published: February 2023

Scientists take fresh approaches to seemingly familiar data in timbers and sediments.
How Thick Is Antarctic Ice, and What Is Underneath?
Authors: Sidik Saima
Published: February 2023

Scientists used electromagnetic fields to determine the thickness of fast ice.
Historical Changes in Wind‐Driven Ocean Circulation Can Accelerate Global Warming
Authors: McMonigal Kay , Larson Sarah , Hu Shineng , Kramer Ryan
Published: February 2023

Mitigation and adaptation strategies for climate change depend on accurate climate projections for the coming decades. While changes in radiative heat fluxes are known to contribute to surface warming, changes to ocean circulation can also impact the rate of surface warming. Previous studies suggest that projected changes to ocean circulation reduce the rate of global warming. However, these studies consider large greenhouse gas forcing scenarios, which induce a significant buoyancy‐driven decline of the Atlantic Meridional Overturning Circulation. Here, we use a climate model to quantify the previously unknown impact of changes to wind‐driven ocean circulation on global surface warming. Wind‐driven ocean circulation changes amplify the externally forced warming rate by 17% from 1979 to 2014. Accurately simulating changes to the atmospheric circulation is key to improving near‐term climate projections.
Nanoparticle-mediated targeting of the fusion gene RUNX1/ETO in t(8;21)-positive acute myeloid leukaemia
Authors: Issa Hasan , Swart Laura E. , Rasouli Milad , Ashtiani Minoo , Nakjang Sirintra , Jyotsana Nidhi , Schuschel Konstantin , Heuser Michael , Blair Helen , Heidenreich Olaf
Published: February 2023

A hallmark of acute myeloid leukaemias (AMLs) are chromosomal rearrangements that give rise to novel leukaemia-specific fusion genes. Most of these fusion genes are both initiating and driving events in AML and therefore constitute ideal therapeutic targets but are challenging to target by conventional drug development. siRNAs are frequently used for the specific suppression of fusion gene expression but require special formulations for efficient in vivo delivery. Here we describe the use of siRNA-loaded lipid nanoparticles for the specific therapeutic targeting of the leukaemic fusion gene RUNX1/ETO. Transient knockdown of RUNX1/ETO reduces its binding to its target genes and alters the binding of RUNX1 and its co-factor CBFβ. Transcriptomic changes in vivo were associated with substantially increased median survival of a t(8;21)-AML mouse model. Importantly, transient knockdown in vivo causes long-lasting inhibition of leukaemic proliferation and clonogenicity, induction of myeloid differentiation and a markedly impaired re-engraftment potential in vivo. These data strongly suggest that temporary inhibition of RUNX1/ETO results in long-term restriction of leukaemic self-renewal. Our results provide proof for the feasibility of targeting RUNX1/ETO in a pre-clinical setting and support the further development of siRNA-LNPs for the treatment of fusion gene-driven malignancies.
Low-temperature processing of screen-printed piezoelectric KNbO3 with integration onto biodegradable paper substrates
Authors: Monroe Morgan M. , Villanueva L. Guillermo , Briand Danick
Published: February 2023

The development of fully solution-processed, biodegradable piezoelectrics is a critical step in the development of green electronics towards the worldwide reduction of harmful electronic waste. However, recent printing processes for piezoelectrics are hindered by the high sintering temperatures required for conventional perovskite fabrication techniques. Thus, a process was developed to manufacture lead-free printed piezoelectric devices at low temperatures to enable integration with eco-friendly substrates and electrodes. A printable ink was developed for screen printing potassium niobate (KNbO3) piezoelectric layers in microns of thickness at a maximum processing temperature of 120 °C with high reproducibility. Characteristic parallel plate capacitor and cantilever devices were designed and manufactured to assess the quality of this ink and evaluate its physical, dielectric, and piezoelectric characteristics; including a comparison of behaviour between conventional silicon and biodegradable paper substrates. The printed layers were 10.7–11.2 μm thick, with acceptable surface roughness values in the range of 0.4–1.1 μm. The relative permittivity of the piezoelectric layer was 29.3. The poling parameters were optimised for the piezoelectric response, with an average longitudinal piezoelectric coefficient for samples printed on paper substrates measured as d33, eff, paper = 13.57 ± 2.84 pC/N; the largest measured value was 18.37 pC/N on paper substrates. This approach to printable biodegradable piezoelectrics opens the way forward for fully solution-processed green piezoelectric devices.
Biophotoelectrochemical process co-driven by dead microalgae and live bacteria
Authors: Chen Shanshan , Chen Jin , Zhang Lanlan , Huang Shaofu , Liu Xing , Yang Yuting , Luan Tiangang , Zhou Shungui , Nealson Kenneth H , Rensing Christopher
Published: February 2023

Anaerobic reduction processes in natural waters can be promoted by dead microalgae that have been attributed to nutrient substances provided by the decomposition of dead microalgae for other microorganisms. However, previous reports have not considered that dead microalgae may also serve as photosensitizers to drive microbial reduction processes. Here we demonstrate a photoelectric synergistic linkage between dead microalgae and bacteria capable of extracellular electron transfer (EET). Illumination of dead Raphidocelis subcapitata resulted in two-fold increase in the rate of anaerobic bioreduction by pure Geobacter sulfurreducens, suggesting that photoelectrons generated from the illuminated dead microalgae were transferred to the EET-capable microorganisms. Similar phenomena were observed in NO3− reduction driven by irradiated dead Chlorella vulgaris and living Shewanella oneidensis, and Cr(VI) reduction driven by irradiated dead Raphidocelis subcapitata and living Bacillus subtilis. Enhancement of bioreduction was also seen when the killed microalgae were illuminated in mixed-culture lake water, suggesting that EET-capable bacteria were naturally present and this phenomenon is common in post-bloom systems. The intracellular ferredoxin-NADP+-reductase is inactivated in the dead microalgae, allowing the production and extracellular transfer of photoelectrons. The use of mutant strains confirmed that the electron transport pathway requires multiheme cytochromes. Taken together, these results suggest a heretofore overlooked biophotoelectrochemical process jointly mediated by illumination of dead microalgae and live EET-capable bacteria in natural ecosystems, which may add an important component in the energetics of bioreduction phenomena particularly in microalgae-enriched environments.
Seasonal activities of the phyllosphere microbiome of perennial crops
Authors: Howe Adina , Stopnisek Nejc , Dooley Shane K. , Yang Fan , Grady Keara L. , Shade Ashley
Published: February 2023

Understanding the interactions between plants and microorganisms can inform microbiome management to enhance crop productivity and resilience to stress. Here, we apply a genome-centric approach to identify ecologically important leaf microbiome members on replicated plots of field-grown switchgrass and miscanthus, and to quantify their activities over two growing seasons for switchgrass. We use metagenome and metatranscriptome sequencing and curate 40 medium- and high-quality metagenome-assembled-genomes (MAGs). We find that classes represented by these MAGs (Actinomycetia, Alpha- and Gamma- Proteobacteria, and Bacteroidota) are active in the late season, and upregulate transcripts for short-chain dehydrogenase, molybdopterin oxidoreductase, and polyketide cyclase. Stress-associated pathways are expressed for most MAGs, suggesting engagement with the host environment. We also detect seasonally activated biosynthetic pathways for terpenes and various non-ribosomal peptide pathways that are poorly annotated. Our findings support that leaf-associated bacterial populations are seasonally dynamic and responsive to host cues.
Malaria-driven adaptation of MHC class I in wild bonobo populations
Authors: Wroblewski Emily E. , Guethlein Lisbeth A. , Anderson Aaron G. , Liu Weimin , Li Yingying , Heisel Sara E. , Connell Andrew Jesse , Ndjango Jean-Bosco N. , Bertolani Paco , Hart John A. , Hart Terese B. , Sanz Crickette M. , Morgan David B. , Peeters Martine , Sharp Paul M. , Hahn Beatrice H. , Parham Peter
Published: February 2023

The malaria parasite Plasmodium falciparum causes substantial human mortality, primarily in equatorial Africa. Enriched in affected African populations, the B*53 variant of HLA-B, a cell surface protein that presents peptide antigens to cytotoxic lymphocytes, confers protection against severe malaria. Gorilla, chimpanzee, and bonobo are humans’ closest living relatives. These African apes have HLA-B orthologs and are infected by parasites in the same subgenus (Laverania) as P. falciparum, but the consequences of these infections are unclear. Laverania parasites infect bonobos (Pan paniscus) at only one (TL2) of many sites sampled across their range. TL2 spans the Lomami River and has genetically divergent subpopulations of bonobos on each side. Papa-B, the bonobo ortholog of HLA-B, includes variants having a B*53-like (B07) peptide-binding supertype profile. Here we show that B07 Papa-B occur at high frequency in TL2 bonobos and that malaria appears to have independently selected for different B07 alleles in the two subpopulations.
THADA inhibition in mice protects against type 2 diabetes mellitus by improving pancreatic β-cell function and preserving β-cell mass
Authors: Zhang Yuqing , Han Shan , Liu Congcong , Zheng Yuanwen , Li Hao , Gao Fei , Bian Yuehong , Liu Xin , Liu Hongbin , Hu Shourui , Li Yuxuan , Chen Zi-Jiang , Zhao Shigang , Zhao Han
Published: February 2023

Impaired insulin secretion is a hallmark in type 2 diabetes mellitus (T2DM). THADA has been identified as a candidate gene for T2DM, but its role in glucose homeostasis remains elusive. Here we report that THADA is strongly activated in human and mouse islets of T2DM. Both global and β-cell-specific Thada-knockout mice exhibit improved glycemic control owing to enhanced β-cell function and decreased β-cell apoptosis. THADA reduces endoplasmic reticulum (ER) Ca2+ stores in β-cells by inhibiting Ca2+ re-uptake via SERCA2 and inducing Ca2+ leakage through RyR2. Upon persistent ER stress, THADA interacts with and activates the pro-apoptotic complex comprising DR5, FADD and caspase-8, thus aggravating ER stress-induced apoptosis. Importantly, THADA deficiency protects mice from high-fat high-sucrose diet- and streptozotocin-induced hyperglycemia by restoring insulin secretion and preserving β-cell mass. Moreover, treatment with alnustone inhibits THADA’s function, resulting in ameliorated hyperglycemia in obese mice. Collectively, our results support pursuit of THADA as a potential target for developing T2DM therapies.
Responsive materials and mechanisms as thermal safety systems for skin-interfaced electronic devices
Authors: Yoo Seonggwang , Yang Tianyu , Park Minsu , Jeong Hyoyoung , Lee Young Joong , Cho Donghwi , Kim Joohee , Kwak Sung Soo , Shin Jaeho , Park Yoonseok , Wang Yue , Miljkovic Nenad , King William P. , Rogers John A.
Published: February 2023

Soft, wireless physiological sensors that gently adhere to the skin are capable of continuous clinical-grade health monitoring in hospital and/or home settings, of particular value to critically ill infants and other vulnerable patients, but they present risks for injury upon thermal failure. This paper introduces an active materials approach that automatically minimizes such risks, to complement traditional schemes that rely on integrated sensors and electronic control circuits. The strategy exploits thin, flexible bladders that contain small volumes of liquid with boiling points a few degrees above body temperature. When the heat exceeds the safe range, vaporization rapidly forms highly effective, thermally insulating structures and delaminates the device from the skin, thereby eliminating any danger to the skin. Experimental and computational thermomechanical studies and demonstrations in a skin-interfaced mechano-acoustic sensor illustrate the effectiveness of this simple thermal safety system and suggest its applicability to nearly any class of skin-integrated device technology.
Time-series quantum reservoir computing with weak and projective measurements
Authors: Mujal Pere , Martínez-Peña Rodrigo , Giorgi Gian Luca , Soriano Miguel C. , Zambrini Roberta
Published: February 2023

Time-series processing is a major challenge in machine learning with enormous progress in the last years in tasks such as speech recognition and chaotic series prediction. A promising avenue for sequential data analysis is quantum machine learning, with computational models like quantum neural networks and reservoir computing. An open question is how to efficiently include quantum measurement in realistic protocols while retaining the needed processing memory and preserving the quantum advantage offered by large Hilbert spaces. In this work, we propose different measurement protocols and assess their efficiency in terms of resources, through theoretical predictions and numerical analysis. We show that it is possible to exploit the quantumness of the reservoir and to obtain ideal performance both for memory and forecasting tasks with two successful measurement protocols. One repeats part of the experiment after each projective measurement while the other employs weak measurements operating online at the trade-off where information can be extracted accurately and without hindering the needed memory, in spite of back-action effects. Our work establishes the conditions for efficient time-series processing paving the way to its implementation in different quantum technologies.
Preserved electrophysiological markers of confidence in schizophrenia spectrum disorder
Authors: Rouy Martin , Roger Matthieu , Goueytes Dorian , Pereira Michael , Roux Paul , Faivre Nathan
Published: February 2023

A large number of behavioral studies suggest that confidence judgments are impaired in schizophrenia, motivating the search for neural correlates of an underlying metacognitive impairment. Electrophysiological studies suggest that a specific evoked response potential reflecting performance monitoring, namely the error-related negativity (ERN), is blunted in schizophrenia compared to healthy controls. However, attention has recently been drawn to a potential confound in the study of metacognition, namely that lower task-performance in schizophrenia compared to healthy controls involves a decreased index of metacognitive performance (where metacognitive performance is construed as the ability to calibrate one’s confidence relative to response correctness), independently of metacognitive abilities among patients. Here, we assessed how this confound might also apply to ERN-blunting in schizophrenia. We used an adaptive staircase procedure to titrate task-performance on a motion discrimination task in which participants (N = 14 patients and 19 controls) had to report their confidence after each trial while we recorded high density EEG. Interestingly, not only metaperceptual abilities were preserved among patients at the behavioral level, but contrary to our hypothesis, we also found no electrophysiological evidence for altered EEG markers of performance monitoring. These results bring additional evidence suggesting an unaltered ability to monitor perceptual performance on a trial by trial basis in schizophrenia.
Mycobacterial nucleoid-associated protein Lsr2 is required for productive mycobacteriophage infection
Authors: Dulberger Charles L. , Guerrero-Bustamante Carlos A. , Owen Siân V. , Wilson Sean , Wuo Michael G. , Garlena Rebecca A. , Serpa Lexi A. , Russell Daniel A. , Zhu Junhao , Braunecker Ben J. , Squyres Georgia R. , Baym Michael , Kiessling Laura L. , Garner Ethan C. , Rubin Eric J. , Hatfull Graham F.
Published: February 2023

Mycobacteriophages are a diverse group of viruses infecting Mycobacterium with substantial therapeutic potential. However, as this potential becomes realized, the molecular details of phage infection and mechanisms of resistance remain ill-defined. Here we use live-cell fluorescence microscopy to visualize the spatiotemporal dynamics of mycobacteriophage infection in single cells and populations, showing that infection is dependent on the host nucleoid-associated Lsr2 protein. Mycobacteriophages preferentially adsorb at Mycobacterium smegmatis sites of new cell wall synthesis and following DNA injection, Lsr2 reorganizes away from host replication foci to establish zones of phage DNA replication (ZOPR). Cells lacking Lsr2 proceed through to cell lysis when infected but fail to generate consecutive phage bursts that trigger epidemic spread of phage particles to neighbouring cells. Many mycobacteriophages code for their own Lsr2-related proteins, and although their roles are unknown, they do not rescue the loss of host Lsr2.
Phase-locked photon–electron interaction without a laser
Authors: Taleb Masoud , Hentschel Mario , Rossnagel Kai , Giessen Harald , Talebi Nahid
Published: February 2023

Ultrafast photon–electron spectroscopy in electron microscopes commonly requires ultrafast laser setups. Photoemission from an engineered electron source is used to generate pulsed electrons, interacting with a sample excited by the laser pulse at a known time delay. Thus, developing an ultrafast electron microscope demands the exploitation of extrinsic laser excitations and complex synchronization schemes. Here we present an inverse approach to introduce internal radiation sources in an electron microscope based on cathodoluminescence spectroscopy. Our compact method is based on a sequential interaction of the electron beam with an electron-driven photon source and the investigated sample. Such a source in an electron microscope generates phase-locked photons that are mutually coherent with the near-field distribution of the swift electron. We confirm the mutual frequency and momentum-dependent correlation of the electron-driven photon source and sample radiation and determine a degree of mutual coherence of up to 27%. With this level of mutual coherence, we were able to perform spectral interferometry with an electron microscope. Our method has the advantage of being simple, compact and operating with continuous electron beams. It will open the door to local photon–electron correlation spectroscopy of quantum materials, single-photon systems and coherent exciton–polaritonic samples with nanometre resolution.
Bile metabolites as diagnostic biomarkers for perihilar cholangiocarcinoma
Authors: Zhang DengYong , Zhao GuanRu , Sun Wanliang , Wang Dongdong , Zhou Shuo , Liu Zhong , Lu Zheng
Published: February 2023

It is difficult to directly obtain pathological diagnosis of perihilar cholangiocarcinoma (pCCA). Analysis of bile in the pCCA microenvironment, based on metabolomics and statistical methods, can help in clinical diagnosis. Clinical information, bile samples, blood liver function, blood CA199, CEA, and other indicators were collected from 33 patients with pCCA and 16 patients with gallstones. Bile samples were analyzed using untargeted metabolomics methods. A combination of multivariate and univariate analyses were used to screen for potential differential metabolites Through Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment and differential metabolite remodeling, we explored changes in the pCCA pathway and potential therapeutic targets. There were significant differences in patient blood TBIL, ALT, AST, TBA, CA19-9, and CEA indices (p < 0.05, |log2(fc)| ≥ 1) between two groups. A significant correlation was found between these different indicators by Spearman's analysis. The clinical parameters were correlated with mass-to-charge ratios of 305 (Positive Ion Mode, POS) and 246 (Negative Ion Mode, NEG) in the metabolic group (|r| ≥ 0.7, P ≤ 10−7). The result of this study indicated that bile untargeted metabolomics combined with statistical analysis techniques may be used for diagnose and treatment of pCCA.
Implicit feedback policies for COVID-19: why “zero-COVID” policies remain elusive
Authors: Jadbabaie Ali , Sarker Arnab , Shah Devavrat
Published: February 2023

Successful epidemic modeling requires understanding the implicit feedback control strategies used by populations to modulate the spread of contagion. While such strategies can be replicated with intricate modeling assumptions, here we propose a simple model where infection dynamics are described by a three parameter feedback policy. Rather than model individuals as directly controlling the contact rate which governs the spread of disease, we model them as controlling the contact rate’s derivative, resulting in a dynamic rather than kinematic model. The feedback policy used by populations across the United States which best fits observations is proportional-derivative control, where learned parameters strongly correlate with observed interventions (e.g., vaccination rates and mobility restrictions). However, this results in a non-zero “steady-state” of case counts, implying current mitigation strategies cannot eradicate COVID-19. Hence, we suggest making implicit policies a function of cumulative cases, resulting in proportional-integral-derivative control with higher potential to eliminate COVID-19.
Biomechanical consequences of the intervertebral disc centre of rotation kinematics during lateral bending and axial rotation
Authors: Allais Roman , Capart Antoine , Da Silva Anabela , Boiron Olivier
Published: February 2023

The location of the instantaneous centre of rotation (ICR) of a lumbar unit has a considerable clinical importance as a spinal health estimator. Consequently, many studies have been conducted to measure or estimate the ICR during rotations in the three anatomical planes; however the results reported are widely scattered. Even if some inter-subjects variability is to be expected, such inconsistencies are likely explained by the differences in methods and experiments. Therefore, in this paper we seek to model three behaviours of the ICR during lateral bending and axial rotation based on results published in the literature. In order to assess the metabolic and mechanical sensibility to the assumption made on the ICR kinematics, we used a previously validated three dimensional non-linear poroelastic model of a porcine intervertebral disc to simulate physiological lateral and axial rotations. The impact of the geometry was also briefly investigated by considering a 11$$^\circ$$

∘

wedge angle. From our simulations, it appears that the hypothesis made on the ICR location does not significantly affect the critical nutrients concentrations but gives disparate predictions of the intradiscal pressure at the centre of the disc (variation up to 0.7 MPa) and of the displacement fields (variation up to 0.4 mm). On the contrary, the wedge angle does not influence the estimated intradiscal pressure but leads to minimal oxygen concentration decreased up to 33% and increased maximal lactate concentration up to 13%. While we can not settle on which definition of the ICR is more accurate, this work suggests that patient-specific modeling of the ICR is required and brings new insights that can be useful for the development of new tools or the design of surgical material such as total lumbar disc prostheses.
Uncertainty in vulnerability of networks under attack
Authors: Ermagun Alireza , Tajik Nazanin , Mahmassani Hani
Published: February 2023

This study builds conceptual explanations and empirical examinations of the vulnerability response of networks under attack. Two quantities of “vulnerability” and “uncertainty in vulnerability” are defined by scrutinizing the performance loss trajectory of networks experiencing attacks. Both vulnerability and uncertainty in vulnerability quantities are a function of the network topology and size. This is tested on 16 distinct topologies appearing in infrastructure, social, and biological networks with 8 to 26 nodes under two percolation scenarios exemplifying benign and malicious attacks. The findings imply (i) crossing path, tree, and diverging tail are the most vulnerable topologies, (ii) complete and matching pairs are the least vulnerable topologies, (iii) complete grid and complete topologies show the most uncertainty for vulnerability, and (iv) hub-and-spoke and double u exhibit the least uncertainty in vulnerability. The findings also imply that both vulnerability and uncertainty in vulnerability increase with an increase in the size of the network. It is argued that in networks with no undirected cycle and one undirected cycle, the uncertainty in vulnerability is maximal earlier in the percolation process. With an increase in the number of cycles, the uncertainty in vulnerability is accumulated at the end of the percolation process. This emphasizes the role of tailoring preparedness, response, and recovery phases for networks with different topologies when they might experience disruption.