bluuebunny/crossref_metadata_2025 huggingface dataset:
Circulating tears and managing hearts: Governing through affect in an Indonesian steel factory
Authors: Rudnyckyj Daromir
Published: April 2011

This article argues that moderate Islamic spiritual training programs in contemporary Indonesia entail ‘governing through affect’. This formulation captures the embodied dispositions and ritual forms through which affect is mobilized to serve as a modality of government. Based on over two years of ethnographic research in Indonesia, most of which took place at Krakatau Steel in western Java, I examine the affective incitements that took place in ritual settings dedicated toward corporate productivity and self-improvement. The article argues that this process of subjectification took place in three stages. First, what participants referred to as ‘opening the heart’, which involved making participants receptive to the message of work as worship through recourse to affective enactments in Islamic history and discourse. Second, the circulation of tears, which refers to deep collective weeping which simultaneously represented and physically enacted the adoption of a new subjectivity. Finally, ‘managing the heart’ — the ultimate goal of spiritual training — was a form of self-management in which one exercised ‘built-in control’ and acted in ways that were deemed simultaneously conducive to corporate competitiveness and other-worldly salvation. I conclude that affect constitutes the virtuality of ritual insofar as it is the medium through which spiritual reform enters into the vital processes of human life.
Prototyping self in Silicon Valley: Deep diversity as a framework for anthropological inquiry
Authors: English-Lueck J.A.
Published: April 2011

High-technology work fuels a dynamic global exchange from technopoles throughout the world, but especially between East and South Asia and the northern Californian region of Silicon Valley. This migration drives an expanded number of ancestral identities. Professional and activity-based identities flourish as Silicon Valley’s strong narrative of meritocracy loosens the grip of birth ascription on the creation of identities. These achieved identities proliferate as people experiment on their own sense of self. Traditional conceptual tools related to immigration, and even such contemporary approaches as Appadurai’s ethnoscapes, do not adequately illuminate the ethnographic data on Silicon Valley workers, families, and especially youth. The concept of deep diversity, first posed by philosopher Charles Taylor and reified by anthropologist Clifford Geertz, reinterprets the interactions of traditional ethnic identity categories, providing a powerful framework with which to think.
Heat-shock protein 90 inhibition in autoimmunity to type VII collagen: evidence that nonmalignant plasma cells are not therapeutic targets
Authors: Kasperkiewicz Michael , Müller Ralf , Manz Rudolf , Magens Moritz , Hammers Christoph M. , Somlai Csaba , Westermann Jürgen , Schmidt Enno , Zillikens Detlef , Ludwig Ralf J. , Orosz Antal
Published: April 2011

Blocking heat-shock protein 90 (Hsp90) induces death of malignant plasma cells by activation of the unfolded protein response, a signaling pathway activated by accumulation of misfolded proteins within the endoplasmic reticulum. We hypothesized that nontransformed plasma cells are also hypersensitive to Hsp90 inhibition because of their high amount of protein biosynthesis. To investigate this hypothesis, 2 different Hsp90 inhibitors, the geldanamycin derivative 17-DMAG and the nontoxic peptide derivative TCBL-145, were applied to mice with experimental epidermolysis bullosa acquisita, an autoimmune bullous disease characterized by autoantibodies against type VII collagen of the dermal-epidermal junction. Both inhibitors ameliorated clinical disease of type VII collagen–immunized mice, suppressed auto-antibody production, and reduced dermal neutrophilic infiltrate. Interestingly, total plasma cell numbers, type VII collagen–specific plasma cells, and germinal center B cells were unaffected by anti-Hsp90 treatment in vivo. However, T-cell proliferation was potently inhibited, as evidenced by the reduced response of isolated lymph node cells from immunized mice to in vitro restimulation with anti-CD3/CD28 antibody or autoantigen in the presence of Hsp90 inhibitors. Our results suggest that Hsp90 blockade has no impact on normal or autoreactive plasma cells in vivo and indentify T cells as targets of anti-Hsp90 treatment in autoimmunity to type VII collagen.
A new pathway of glucocorticoid action for asthma treatment through the regulation of PTEN expression
Authors: Ni ZhenHua , Tang JiHong , Cai ZhuYing , Yang Wei , Zhang Lei , Chen Qingge , Zhang Long , Wang XiongBiao
Published: April 2011

Background

"Phosphatase and tensin homolog deleted on chromosome 10" (PTEN) is mostly considered to be a cancer-related gene, and has been suggested to be a new pathway of pathogenesis of asthma. The purpose of this study was to investigate the effects of the glucocorticoid, dexamethasone, on PTEN regulation.

Methods

OVA-challenged mice were used as an asthma model to investigate the effect of dexamethasone on PTEN regulation. Immunohistochemistry was used to detect expression levels of PTEN protein in lung tissues. The human A549 cell line was used to explore the possible mechanism of action of dexamethasone on human PTEN regulation in vitro. A luciferase reporter construct under the control of PTEN promoter was used to confirm transcriptional regulation in response to dexamethasone.

Results

PTEN protein was found to be expressed at low levels in lung tissues in asthmatic mice; but the expression was restored after treatment with dexamethasone. In A549 cells, human PTEN was up-regulated by dexamethasone treatment. The promoter-reporter construct confirmed that dexamethasone could regulate human PTEN transcription. Treatment with the histone deacetylase inhibitor, TSA, could increase PTEN expression in A549 cells, while inhibition of histone acetylase (HAT) by anacardic acid attenuated dexamethasone-induced PTEN expression.

Conclusions

Based on the data a new mechanism is proposed where glucocorticoids treat asthma partly through up-regulation of PTEN expression. The in vitro studies also suggest that the PTEN pathway may be involved in human asthma.
Comparative supragenomic analyses among the pathogens Staphylococcus aureus, Streptococcus pneumoniae, and Haemophilus influenzae Using a modification of the finite supragenome model
Authors: Boissy Robert , Ahmed Azad , Janto Benjamin , Earl Josh , Hall Barry G , Hogg Justin S , Pusch Gordon D , Hiller Luisa N , Powell Evan , Hayes Jay , Yu Susan , Kathju Sandeep , Stoodley Paul , Post J Christopher , Ehrlich Garth D , Hu Fen Z
Published: April 2011

Background

Staphylococcus aureus is associated with a spectrum of symbiotic relationships with its human host from carriage to sepsis and is frequently associated with nosocomial and community-acquired infections, thus the differential gene content among strains is of interest.

Results

We sequenced three clinical strains and combined these data with 13 publically available human isolates and one bovine strain for comparative genomic analyses. All genomes were annotated using RAST, and then their gene similarities and differences were delineated. Gene clustering yielded 3,155 orthologous gene clusters, of which 2,266 were core, 755 were distributed, and 134 were unique. Individual genomes contained between 2,524 and 2,648 genes. Gene-content comparisons among all possible S. aureus strain pairs (n = 136) revealed a mean difference of 296 genes and a maximum difference of 476 genes. We developed a revised version of our finite supragenome model to estimate the size of the S. aureus supragenome (3,221 genes, with 2,245 core genes), and compared it with those of Haemophilus influenzae and Streptococcus pneumoniae. There was excellent agreement between RAST's annotations and our CDS clustering procedure providing for high fidelity metabolomic subsystem analyses to extend our comparative genomic characterization of these strains.

Conclusions

Using a multi-species comparative supragenomic analysis enabled by an improved version of our finite supragenome model we provide data and an interpretation explaining the relatively larger core genome of S. aureus compared to other opportunistic nasopharyngeal pathogens. In addition, we provide independent validation for the efficiency and effectiveness of our orthologous gene clustering algorithm.
Activated IL-23/IL-17 pathway closely correlates with increased Foxp3 expression in livers of chronic hepatitis B patients
Authors: Wang Qinghong , Zheng Yanhua , Huang Zemin , Tian Yi , Zhou Jijun , Mao Qing , Wu Yuzhang , Ni Bing
Published: April 2011

Background

Foxp3 protein plays a critical role in mediating the inflammatory response and can inhibit the proinflammatory IL-23/IL-17 pathway. However, the molecular interplay of Foxp3 and the IL-23/IL-17 pathway in patients with chronic hepatitis B (CHB) remains unclear. To this end, we analyzed the expression patterns of Foxp3- and IL-23/IL-17 pathway-related proinflammatory cytokines in 39 patients with acute-on-chronic liver failure, 71 patients with CHB and 32 healthy controls.

Results

Foxp3 expression was found to be elevated in and mainly expressed by the CD4+ T cell sub-population of peripheral blood mononuclear cells and liver tissues of patients with hepatitis B. The intrahepatic expression of Foxp3 strongly correlated with the copies of HBV DNA and the concentration of surface antigen, HBsAg. IL-23/IL-17 pathway-related proinflammatory cytokines were also found to be significantly increased in patients' liver tissues, as compared to healthy controls. Moreover, Foxp3 expression was strikingly correlated with the production of these cytokines in liver tissues of CHB patients.

Conclusions

The closely-correlated increase of Foxp3 and IL-23/IL-17 pathway activity in HBV-infected livers suggests that the proinflammatory IL-23/IL-17 pathway had not been effectively suppressed by the host immune machinery, such as Treg (Foxp3) cells. Constitutive activation of the IL-23/17 pathway, thus, may support the chronic hepatitis B state.
Self-rated health in rural Appalachia: health perceptions are incongruent with health status and health behaviors
Authors: Griffith Brian N , Lovett Gretchen D , Pyle Donald N , Miller Wayne C
Published: April 2011

Background

Appalachia is characterized by poor health behaviors, poor health status, and health disparities. Recent interventions have not demonstrated much success in improving health status or reducing health disparities in the Appalachian region. Since one's perception of personal health precedes his or her health behaviors, the purpose of this project was to evaluate the self-rated health of Appalachian adults in relation to objective health status and current health behaviors.

Methods

Appalachian adults (n = 1,576) were surveyed regarding health behaviors - soda consumer (drink ≥ 355 ml/d), or non-consumer (drink < 355 ml/d), fast food consumer (eating fast food ≥ 3 times/wk) or healthy food consumer (eating fast food < 3 times/wk), smoking (smoker or non-smoker), exercise (exerciser > 30 min > 1 d/wk) and sedentary (exercise < 30 min 1 d/wk), blood pressure medication (yes, no), and self-rated health. Blood pressure was measured through auscultation and serum cholesterol measured via needle prick. Weight status was based on BMI: normal weight (NW ≥ 18.5 and < 25.0), overweight (OW ≥ 25.0 and < 30.0), and obese (OB ≥ 30.0). Jaccard Binary Similarity coefficients, odds ratios, chi-square, and prevalence ratios were calculated to evaluate the relationships among self-rated health, objective health status, and health behaviors. Significance was set at p < 0.05.

Results

Respondents reported being healthy, while being sedentary (65%), hypertensive (76%), overweight (73%), or hyperlipidemic (79%). Between 57% and 66% of the respondents who considered themselves healthy had at least two disease conditions or poor health behaviors. Jaccard Binary Similarity coefficients and odds ratios showed the probability of reporting being healthy when having a disease condition or poor health behavior was high.

Conclusions

The association between self-rated health and poor health indicators in Appalachian adults is distorted. The public health challenge is to formulate messages and programs about health and health needs which take into account the current distortion about health in Appalachia and the cultural context in which this distortion was shaped.
Effect of breastfeeding promotion interventions on breastfeeding rates, with special focus on developing countries
Authors: Imdad Aamer , Yakoob Mohammad Yawar , Bhutta Zulfiqar A
Published: April 2011

Background

Given the recognized benefits of breastfeeding for the health of the mother and infants, the World Health Organization (WHO) recommends exclusive breastfeeding (EBF) for the first six months of life. However, the prevalence of EBF is low globally in many of the developing and developed countries around the world. There is much interest in the effectiveness of breastfeeding promotion interventions on breastfeeding rates in early infancy.

Methods

A systematic literature was conducted to identify all studies that evaluated the impact of breastfeeding promotional strategies on any breastfeeding and EBF rates at 4-6 weeks and at 6 months. Data were abstracted into a standard excel sheet by two authors. Meta-analyses were performed with different sub-group analyses. The overall evidence were graded according to the Child Health Epidemiology Reference Group (CHERG) rules using the adapted Grading of Recommendations, Assessment, Development and Evaluation (GRADE) criteria and recommendations made from developing country studies for inclusion into the Live Saved Tool (LiST) model.

Results

After reviewing 968 abstracts, 268 studies were selected for potential inclusion, of which 53 randomized and quasi-randomized controlled trials were selected for full abstraction. Thirty two studies gave the outcome of EBF at 4-6 weeks postpartum. There was a statistically significant 43% increase in this outcome, with 89% and 20% significant increases in developing and developed countries respectively. Fifteen studies reported EBF outcomes at 6 months. There was an overall 137% increase, with a significant 6 times increase in EBF in developing countries, compared to 1.3 folds increase in developed country studies. Further sub-group analyses proved that prenatal counseling had a significant impact on breastfeeding outcomes at 4-6 weeks, while both prenatal and postnatal counseling were important for EBF at 6 months.

Conclusion

Breastfeeding promotion interventions increased exclusive and any breastfeeding rates at 4-6 weeks and at 6 months. A relatively greater impact of these interventions was seen in developing countries with 1.89 and 6 folds increase in EBF rates at 4-6 weeks and at 6 months respectively.
Does primary brachial plexus surgery alter palliative tendon transfer surgery outcomes in children with obstetric paralysis?
Authors: Aydın Atakan , Biçer Ahmet , Özkan Türker , Mersa Berkan , Özkan Safiye , Yıldırım Zeynep Hoşbay
Published: April 2011

Background

The surgical management of obstetrical brachial plexus palsy can generally be divided into two groups; early reconstructions in which the plexus or affected nerves are addressed and late or palliative reconstructions in which the residual deformities are addressed. Tendon transfers are the mainstay of palliative surgery. Occasionally, surgeons are required to utilise already denervated and subsequently reinnervated muscles as motors. This study aimed to compare the outcomes of tendon transfers for residual shoulder dysfunction in patients who had undergone early nerve surgery to the outcomes in patients who had not.

Methods

A total of 91 patients with obstetric paralysis-related shoulder abduction and external rotation deficits who underwent a modified Hoffer transfer of the latissimus dorsi/teres major to the greater tubercle of the humerus tendon between 2002 and 2009 were retrospectively analysed. The patients who had undergone neural surgery during infancy were compared to those who had not in terms of their preoperative and postoperative shoulder abduction and external rotation active ranges of motion.

Results

In the early surgery groups, only the postoperative external rotation angles showed statistically significant differences (25 degrees and 75 degrees for total and upper type palsies, respectively). Within the palliative surgery-only groups, there were no significant differences between the preoperative and postoperative abduction and external rotation angles. The significant differences between the early surgery groups and the palliative surgery groups with total palsy during the preoperative period diminished postoperatively (p < 0.05 and p > 0.05, respectively) for abduction but not for external rotation. Within the upper type palsy groups, there were no significant differences between the preoperative and postoperative abduction and external rotation angles.

Conclusions

In this study, it was found that in patients with total paralysis, satisfactory shoulder abduction values can be achieved with tendon transfers regardless of a previous history of neural surgery even if the preoperative values differ.
Longitudinal ambulatory measurements of gait abnormality in dystrophin-deficient dogs
Authors: Barthélémy Inès , Barrey Eric , Aguilar Pablo , Uriarte Ane , Le Chevoir Matthias , Thibaud Jean-Laurent , Voit Thomas , Blot Stéphane , Hogrel Jean-Yves
Published: April 2011

Background

This study aimed to measure the gait abnormalities in GRMD (Golden retriever muscular dystrophy) dogs during growth and disease progression using an ambulatory gait analyzer (3D-accelerometers) as a possible tool to assess the effects of a therapeutic intervention.

Methods

Six healthy and twelve GRMD dogs were evaluated twice monthly, from the age of two to nine months. The evolution of each gait variable previously shown to be modified in control and dystrophin-deficient adults was assessed using two-ways variance analysis (age, clinical status) with repeated measurements. A principal component analysis (PCA) was applied to perfect multivariate data interpretation.

Results

Speed, stride length, total power and force significantly already decreased (p < 0.01) at the age of 2 months. The other gait variables (stride frequency, relative power distributions along the three axes) became modified at later stages. Using the PCA analysis, a global gait index taking into account the main gait variables was calculated, and was also consistent to detect the early changes in the GRMD gait patterns, as well as the progressive degradation of gait quality.

Conclusion

The gait variables measured by the accelerometers were sensitive to early detect and follow the gait disorders and mirrored the heterogeneity of clinical presentations, giving sense to monitor gait in GRMD dogs during progression of the disease and pre-clinical therapeutic trials.
Enhanced recovery in colorectal surgery: a multicentre study
Authors: Ramírez José M , Blasco Juan A , Roig José V , Maeso-Martínez Sergio , Casal José E , Esteban Fernando , Lic Daniel Callejo ,   Spanish working group on fast track surgery
Published: April 2011

Background

Major colorectal surgery usually requires a hospital stay of more than 12 days. Inadequate pain management, intestinal dysfunction and immobilisation are the main factors associated with delay in recovery. The present work assesses the short and medium term results achieved by an enhanced recovery program based on previously published protocols.

Methods

This prospective study, performed at 12 Spanish hospitals in 2008 and 2009, involved 300 patients. All patients underwent elective colorectal resection for cancer following an enhanced recovery program. The main elements of this program were: preoperative advice, no colon preparation, provision of carbohydrate-rich drinks one day prior and on the morning of surgery, goal directed fluid administration, body temperature control during surgery, avoiding drainages and nasogastric tubes, early mobilisation, and the taking of oral fluids in the early postoperative period. Perioperative morbidity and mortality data were collected and the length of hospital stay and protocol compliance recorded.

Results

The median age of the patients was 68 years. Fifty-two % of the patients were women. The distribution of patients by ASA class was: I 10%, II 50% and III 40%. Sixty-four % of interventions were laparoscopic; 15% required conversion to laparotomy. The majority of patients underwent sigmoidectomy or right hemicolectomy. The overall compliance to protocol was approximately 65%, but varied widely in its different components. The median length of postoperative hospital stay was 6 days. Some 3% of patients were readmitted to hospital after discharge; some 7% required repeat surgery during their initial hospitalisation or after readmission. The most common complications were surgical (24%), followed by septic (11%) or other medical complications (10%). Three patients (1%) died during follow-up. Some 31% of patients suffered symptoms that delayed their discharge, the most common being vomiting or nausea (12%), dyspnoea (7%) and fever (5%).

Conclusion

The following of this enhanced recovery program posed no risk to patients in terms of morbidity, mortality and shortened the length of their hospital stay. Overall compliance to protocol was 65%. The following of this program was of benefit to patients and reduces costs by shortening the length of hospital stay. The implantation of such programmes is therefore highly recommended.
Solvothermal synthesis and thermoelectric properties of indium telluride nanostring-cluster hierarchical structures
Authors: Tai Guo'an , Miao Chunyang , Wang Yubo , Bai Yunrui , Zhang Haiqian , Guo Wanlin
Published: April 2011

A simple solvothermal approach has been developed to successfully synthesize n-type α-In2Te3 thermoelectric nanomaterials. The nanostring-cluster hierarchical structures were prepared using In(NO3)3 and Na2TeO3 as the reactants in a mixed solvent of ethylenediamine and ethylene glycol at 200°C for 24 h. A diffusion-limited reaction mechanism was proposed to explain the formation of the hierarchical structures. The Seebeck coefficient of the bulk pellet pressed by the obtained samples exhibits 43% enhancement over that of the corresponding thin film at room temperature. The electrical conductivity of the bulk pellet is one to four orders of magnitude higher than that of the corresponding thin film or p-type bulk sample. The synthetic route can be applied to obtain other low-dimensional semiconducting telluride nanostructures.

PACS: 65.80.-g, 68.35.bg, 68.35.bt
Flame synthesis of carbon nanostructures on Ni-plated hardmetal substrates
Authors: Zhu Hongmei , Kuang Tongchun , Zhu Bin , Lei Shumei , Liu Zongwen , Ringer Simon Peter
Published: April 2011

In this article, we demonstrate that carbon nanostructures could be synthesized on the Ni-plated YG6 (WC-6 wt% Co) hardmetal substrate by a simple ethanol diffusion flame method. The morphologies and microstructures of the Ni-plated layer and the carbon nanostructures were examined by various techniques including scanning electron microscopy, X-ray diffraction, and Raman spectroscopy. The growth mechanism of such carbon nanostructures is discussed. This work may provide a strategy to improve the performance of hardmetal products and thus to widen their potential applications.
Opposing roles of PlexinA and PlexinB in axonal branch and varicosity formation
Authors: Neufeld Shay Q , Hibbert Alexa D , Chen Brian E
Published: April 2011

Establishing precise synaptic connectivity during development is crucial for neural circuit function. However, very few molecules have been identified that are involved in determining where and how many synapses form. The Plexin cell-surface molecules are a conserved family of axon guidance receptors that mediate axon fasciculation and repulsion during neural development, and later in development PlexinA receptors are involved in eliminating axonal branches and synapse numbers. Here we investigate the roles of PlexinA and PlexinB receptors in axonal branch and varicosity formation in Drosophila. We knocked down PlexinA or PlexinB expression using RNAi in identified mechanosensory neurons and analyzed axonal branching patterns and varicosity formations. Reducing PlexinA expression increased the axonal arbor complexity by increasing the number of branches and varicosities along the axon. In contrast, knocking down PlexinB expression decreased morphological complexity by decreasing the number of branches and the overall size of the axonal arbor, but did not reduce the number of varicosities. Our results demonstrate opposing roles for PlexinA and PlexinB in local wiring within a target region, where PlexinA functions to suppress excessive axonal branches and synapses and PlexinB facilitates axonal growth.
A Co-operative Regulation of Neuronal Excitability by UNC-7 Innexin and NCA/NALCN Leak Channel
Authors: Bouhours Magali , Po Michelle D , Gao Shangbang , Hung Wesley , Li Hang , Georgiou John , Roder John C , Zhen Mei
Published: April 2011

Gap junctions mediate the electrical coupling and intercellular communication between neighboring cells. Some gap junction proteins, namely connexins and pannexins in vertebrates, and innexins in invertebrates, may also function as hemichannels. A conserved NCA/Dmα1U/NALCN family cation leak channel regulates the excitability and activity of vertebrate and invertebrate neurons. In the present study, we describe a genetic and functional interaction between the innexin UNC-7 and the cation leak channel NCA in Caenorhabditis elegans neurons. While the loss of the neuronal NCA channel function leads to a reduced evoked postsynaptic current at neuromuscular junctions, a simultaneous loss of the UNC-7 function restores the evoked response. The expression of UNC-7 in neurons reverts the effect of the unc-7 mutation; moreover, the expression of UNC-7 mutant proteins that are predicted to be unable to form gap junctions also reverts this effect, suggesting that UNC-7 innexin regulates neuronal activity, in part, through gap junction-independent functions. We propose that, in addition to gap junction-mediated functions, UNC-7 innexin may also form hemichannels to regulate C. elegans' neuronal activity cooperatively with the NCA family leak channels.
Neonatal Diabetes and Congenital Malabsorptive Diarrhea Attributable to a Novel Mutation in the Human Neurogenin-3 Gene Coding Sequence
Authors: Pinney Sara E. , Oliver-Krasinski Jennifer , Ernst Linda , Hughes Nkecha , Patel Puja , Stoffers Doris A. , Russo Pierre , De León Diva D.
Published: April 2011

Objective:

The aim was to describe the clinical presentation and to characterize the genetic mutation present in a child with congenital malabsorptive diarrhea and neonatal diabetes.

Research Design and Methods:

Clinical data were obtained from chart review. Histopathological characterization of intestinal samples and neurogenin-3 (NEUROG3) sequencing were performed. Expression and function of the mutated NEUROG3 protein were assessed by Western blot analysis and luciferase reporter assay.

Results:

At birth, the proband was small for gestational age. She presented for evaluation with persistent diarrhea and a poor postnatal growth pattern. Although the pancreas was present, serum amylase and fecal elastase levels were decreased, and blood glucose levels were persistently elevated by 5 months of age. Immunostaining of a small intestine biopsy for chromogranin A demonstrated complete absence of neuroendocrine cells. Genetic analysis revealed a nonsense mutation (E123X) in the region encoding helix II of the NEUROG3 gene, leading to premature termination at amino acid 123. The mutated truncated NEUROG3 protein was identified by Western blot analysis. Reporter assays show decreased transactivation of the NEUROD1 promoter by mutant NEUROG3 protein as compared to wild type.

Conclusions:

This report describes a newly identified nonsense mutation in human NEUROG3 that in the homozygous state is associated with neonatal diabetes and malabsorptive diarrhea.
A Longitudinal Analysis of the Durability of the Northern-Midland Dialect Boundary in Ohio
Authors: Thomas Erik R.
Published: April 2011

The boundary between the North and Midland dialects in Ohio represents an unusually clear opportunity to test how well dialect boundaries can persist. For part of this boundary, the original settlement by European Americans was strongly segregated: the area north of the line was heavily dominated by settlers from a hearth running from western New England through upstate New York, while the area south of it was dominated by settlers from a hearth comprising southern Pennsylvania, western Maryland, and northern Virginia and West Virginia. Recordings of Dictionary of American Regional English survey subjects, born 1880–1908, were compared with subjects, born 1970–94, from a new survey. A number of variables involving vowel quality or vowel mergers that are known or suspected to differentiate the North and Midland were analyzed in these subjects' speech. Eight variables showed statistically significant differences according to dialect region. When these variables were combined quantitatively, the patterns that emerged are that the North and Midland remain robustly differentiated but that a transition zone has developed along the boundary.
Education After an Earthquake
Authors: Meehan Sean Ross
Published: April 2011

Emerson's neglected engagement with education, in particular in evidence in an address he gave at a secondary school in June 1837 amidst an economic panic and two months before his more famous “American Scholar” address. His analysis offers insight into our current educational crisis and the foundational cracks that run deeper than economic recession.
Beyond Grammar
Authors: Evans Ann
Published: April 2011

Most of my students arrive in my required freshman writing class full of ideas but lacking the mastery over language needed to express them. Introducing core linguistic concepts can sharpen their writing skills by illustrating how language works, and by heightening their awareness of the role language plays in their lives. These concepts could be seamlessly introduced over a year-long, daily high school class. Lacking that, they could be tucked into university level semester-long classes.
Teaching and (Re)Learning the Rhetoric of Emotion
Authors: Stenberg Shari
Published: April 2011

This article argues for a pedagogy that attends to emotion as a crucial, epistemological component of rhetorical education. After exploring dominant cultural tropes for understanding emotion, I examine examples of how these discourses materialize in popular culture. I then draw from classroom moments to analyze the possibilities for and complexities of studying emotion in the classroom.
Polar-localized NPH3-like proteins regulate polarity and endocytosis of PIN-FORMED auxin efflux carriers
Authors: Furutani Masahiko , Sakamoto Norihito , Yoshida Shuhei , Kajiwara Takahito , Robert Hélène S. , Friml Jiří , Tasaka Masao
Published: April 2011

PIN-FORMED (PIN)-dependent auxin transport is essential for plant development and its modulation in response to the environment or endogenous signals. A NON-PHOTOTROPIC HYPOCOTYL 3 (NPH3)-like protein, MACCHI-BOU 4 (MAB4), has been shown to control PIN1 localization during organ formation, but its contribution is limited. The Arabidopsis genome contains four genes, MAB4/ENP/NPY1-LIKE1 (MEL1), MEL2, MEL3 and MEL4, highly homologous to MAB4. Genetic analysis disclosed functional redundancy between MAB4 and MEL genes in regulation of not only organ formation but also of root gravitropism, revealing that NPH3 family proteins have a wider range of functions than previously suspected. Multiple mutants showed severe reduction in PIN abundance and PIN polar localization, leading to defective expression of an auxin responsive marker DR5rev::GFP. Pharmacological analyses and fluorescence recovery after photo-bleaching experiments showed that mel mutations increase PIN2 internalization from the plasma membrane, but affect neither intracellular PIN2 trafficking nor PIN2 lateral diffusion at the plasma membrane. Notably, all MAB4 subfamily proteins show polar localization at the cell periphery in plants. The MAB4 polarity was almost identical to PIN polarity. Our results suggest that the MAB4 subfamily proteins specifically retain PIN proteins in a polarized manner at the plasma membrane, thus controlling directional auxin transport and plant development.
Endoderm and mesoderm reciprocal signaling mediated by CXCL12 and CXCR4 regulates the migration of angioblasts and establishes the pancreatic fate
Authors: Katsumoto Keiichi , Kume Shoen
Published: April 2011

We have discovered that angioblasts trigger an early inductive event in pancreatic differentiation. This event occurs soon after gastrulation, before the formation of blood vessels. Morphological studies revealed that Lmo2-expressing angioblasts reside in proximity to the somitic mesoderm and the gut endoderm from which pancreatic progenitors arise. The chemokine ligand CXCL12 expressed in the gut endoderm functions to attract the angioblasts that express its receptor CXCR4. Angioblasts then signal back to the gut endoderm to induce Pdx1 expression. Gain-of-function and loss-of-function experiments for CXCL12 and CXCR4 were performed to test their function in blood vessel formation and pancreatic differentiation. The ectopic expression of Cxcl12 in the endoderm attracted the angioblasts and induced ectopic Pdx1 expression, resulting in an expanded pancreatic bud and an increased area of insulin-expressing cells. By contrast, in chick embryos treated with beads soaked in AMD3100, an inhibitor of CXCR4, the migration of angioblasts towards the Cxcl12-expressing gut endoderm was arrested, causing a malformation of blood vessels. This led to the generation of a smaller pancreatic bud and a reduced area of insulin-expressing cells. Taken together, these results indicate that the gut endoderm and angioblasts attract each other through reciprocal CXCL12 and CXCR4 signaling. This has a pivotal role in the fate establishment of the pancreatic progenitor cells and in the potentiation of further differentiation into endocrine β-cells.
Primary cilia control telencephalic patterning and morphogenesis via Gli3 proteolytic processing
Authors: Besse Laurianne , Neti Mariame , Anselme Isabelle , Gerhardt Christoph , Rüther Ulrich , Laclef Christine , Schneider-Maunoury Sylvie
Published: April 2011

Primary cilia have essential functions in vertebrate development and signaling. However, little is known about cilia function in brain morphogenesis, a process that is severely affected in human ciliopathies. Here, we study telencephalic morphogenesis in a mouse mutant for the ciliopathy gene Ftm (Rpgrip1l). We show that the olfactory bulbs are present in an ectopic location in the telencephalon of Ftm−/− fetuses and do not display morphological outgrowth at the end of gestation. Investigating the developmental origin of this defect, we have established that E12.5 Ftm−/− telencephalic neuroepithelial cells lack primary cilia. Moreover, in the anterior telencephalon, the subpallium is expanded at the expense of the pallium, a phenotype reminiscent of Gli3 mutants. This phenotype indeed correlates with a decreased production of the short form of the Gli3 protein. Introduction of a Gli3 mutant allele encoding the short form of Gli3 into Ftm mutants rescues both telencephalic patterning and olfactory bulb morphogenesis, despite the persistence of cilia defects. Together, our results show that olfactory bulb morphogenesis depends on primary cilia and that the essential role of cilia in this process is to produce processed Gli3R required for developmental patterning. Our analysis thus provides the first in vivo demonstration that primary cilia control a developmental process via production of the short, repressor form of Gli3. Moreover, our findings shed light on the developmental origin of olfactory bulb agenesis and of other brain morphogenetic defects found in human diseases affecting the primary cilium.
Ret signalling integrates a craniofacial muscle module during development
Authors: Knight Robert D. , Mebus Katharina , d'Angelo Arturo , Yokoya Kazutomo , Heanue Tiffany ,   Tübingen 2000 Screen Consortium, Roehl Henry
Published: April 2011

An appropriate organisation of muscles is crucial for their function, yet it is not known how functionally related muscles are coordinated with each other during development. In this study, we show that the development of a subset of functionally related head muscles in the zebrafish is regulated by Ret tyrosine kinase signalling. Three genes in the Ret pathway (gfra3, artemin2 and ret) are required specifically for the development of muscles attaching to the opercular bone (gill cover), but not other adjacent muscles. In animals lacking Ret or Gfra3 function, myogenic gene expression is reduced in forming opercular muscles, but not in non-opercular muscles derived from the same muscle anlagen. These animals have a normal skeleton with small or missing opercular muscles and tightly closed mouths. Myogenic defects correlate with a highly restricted expression of artn2, gfra3 and ret in mesenchymal cells in and around the forming opercular muscles. ret+ cells become restricted to the forming opercular muscles and a loss of Ret signalling results in reductions of only these, but not adjacent, muscles, revealing a specific role of Ret in a subset of head muscles. We propose that Ret signalling regulates myogenesis in head muscles in a modular manner and that this is achieved by restricting Ret function to a subset of muscle precursors.
Cardiac neural crest is dispensable for outflow tract septation in Xenopus
Authors: Lee Young-Hoon , Saint-Jeannet Jean-Pierre
Published: April 2011

In vertebrate embryos, cardiac precursor cells of the primary heart field are specified in the lateral mesoderm. These cells converge at the ventral midline to form the linear heart tube, and give rise to the atria and the left ventricle. The right ventricle and the outflow tract are derived from an adjacent population of precursors known as the second heart field. In addition, the cardiac neural crest contributes cells to the septum of the outflow tract to separate the systemic and the pulmonary circulations. The amphibian heart has a single ventricle and an outflow tract with an incomplete spiral septum; however, it is unknown whether the cardiac neural crest is also involved in outflow tract septation, as in amniotes. Using a combination of tissue transplantations and molecular analyses in Xenopus we show that the amphibian outflow tract is derived from a second heart field equivalent to that described in birds and mammals. However, in contrast to what we see in amniotes, it is the second heart field and not the cardiac neural crest that forms the septum of the amphibian outflow tract. In Xenopus, cardiac neural crest cells remain confined to the aortic sac and arch arteries and never populate the outflow tract cushions. This significant difference suggests that cardiac neural crest cell migration into the cardiac cushions is an amniote-specific characteristic, presumably acquired to increase the mass of the outflow tract septum with the evolutionary need for a fully divided circulation.
Regulation of somatic myosin activity by Protein Phosphatase 1β controls Drosophila oocyte polarization
Authors: Sun Yi , Yan Yan , Denef Natalie , Schüpbach Trudi
Published: April 2011

The Drosophila body axes are established in the oocyte during oogenesis. Oocyte polarization is initiated by Gurken, which signals from the germline through the epidermal growth factor receptor (Egfr) to the posterior follicle cells (PFCs). In response the PFCs generate an unidentified polarizing signal that regulates oocyte polarity. We have identified a loss-of-function mutation of flapwing, which encodes the catalytic subunit of Protein Phosphatase 1β (PP1β) that disrupts oocyte polarization. We show that PP1β, by regulating myosin activity, controls the generation of the polarizing signal. Excessive myosin activity in the PFCs causes oocyte mispolarization and defective Notch signaling and endocytosis in the PFCs. The integrated activation of JAK/STAT and Egfr signaling results in the sensitivity of PFCs to defective Notch. Interestingly, our results also demonstrate a role of PP1β in generating the polarizing signal independently of Notch, indicating a direct involvement of somatic myosin activity in axis formation.
Cardiovascular actions of the ghrelin gene-derived peptides and growth hormone-releasing hormone
Authors: Granata Riccarda , Isgaard Jörgen , Alloatti Giuseppe , Ghigo Ezio
Published: April 2011

In 1976, small peptide growth hormone secretagogues (GHSs) were discovered and found to promote growth hormone (GH) release from the pituitary. The GHS receptor (GHS-R) was subsequently cloned, and its endogenous ligand ghrelin was later isolated from the stomach. Ghrelin is a 28-amino acid peptide, whose acylation is essential for binding to GHS-R type 1a and for the endocrine functions, including stimulation of GH secretion and subsequent food intake. Unacylated ghrelin, the other ghrelin form, although devoid of GHS-R binding is an active peptide, sharing many peripheral effects with acylated ghrelin (AG). The ghrelin system is broadly expressed in myocardial tissues, where it exerts different functions. Indeed, ghrelin inhibits cardiomyocyte and endothelial cell apoptosis, and improves left ventricular (LV) function during ischemia–reperfusion (I/R) injury. In rats with heart failure (HF), ghrelin improves LV dysfunction and attenuates the development of cardiac cachexia. Similarly, ghrelin exerts vasodilatory effects in humans, improves cardiac function and decreases systemic vascular resistance in patients with chronic HF. Obestatin is a recently identified ghrelin gene peptide. The physiological role of obestatin and its binding to the putative GPR39 receptor are still unclear, although protective effects have been demonstrated in the pancreas and heart. Similarly to AG, the hypothalamic peptide growth hormone-releasing hormone (GHRH) stimulates GH release from the pituitary, through binding to the GHRH-receptor. Besides its proliferative effects in different cell types, at the cardiovascular level GHRH inhibits cardiomyocyte apoptosis, and reduces infarct size in both isolated rat heart after I/R and in vivo after myocardial infarction. Therefore, both ghrelin and GHRH exert cardioprotective effects, which make them candidate targets for therapeutic intervention in cardiovascular dysfunctions.
Nuclear Power Plants for the Renaissance: Looking into the Crystal Ball
Authors: Rao Atambir
Published: April 2011

A commitment to nuclear power requires a perspective spanning decades and even centuries. Predicting the future is likely to be fraught with rather large uncertainties and individual countries will have different perspectives and crystal balls. However, there are several trends that could impact the growth and direction of nuclear power during the 21st century. These trends include the rate of growth of nuclear power, the practical availability of future nuclear technologies and developments in other energy and industrial sectors. This paper focuses on some of the lessons learnt over the last decades, insights from the ongoing construction and some thoughts on the future of the different nuclear technologies and the activities that the IAEA is involved in these areas.
Innovation in Financing New Nuclear
Authors: Mirrlees-Black Jonathan
Published: April 2011

Global interest in investing in nuclear power is strong because of its potential major contribution to decarbonising electricity supply and increasing energy security of supply. But it is by no means certain that there will be a large increase in installed nuclear capacity. Investors in new nuclear currently face significant risks of cost overruns. In addition, its value in delivering low carbon energy is not necessary reflected in revenues. There is also a constraint to the volume of finance that the sector may attract. I argue here that a new type of global nuclear financing vehicle is needed that would route finance to nuclear power.
Uranium Supply for the Nuclear Future
Authors: Kidd Steve
Published: April 2011

Uranium fuel for nuclear reactors is abundant. Known resources of natural uranium available for mining are considerable, and expanding in line with exploration effort. By-product supplies from phosphates promise to increase these further. Secondary supplies from weapons stockpiles, recycling and re-enrichment of depleted uranium tails also contribute significantly. No shortage of uranium is likely in the foreseeable future. With many leading nuclear countries expecting to deploy nuclear reactors by mid century, a 50-fold increase in energy obtained from uranium is likely from about then. Thorium is also a potential nuclear fuel.
Multidimensional Generalized Functions in Aeroacoustics and Fluid Mechanics-Part 1: Basic Concepts and Operations
Authors: Farassat F. , Myers M. K.
Published: April 2011

This paper is the first part of a three part tutorial on multidimensional generalized functions (GFs) and their applications in aeroacoustics and fluid mechanics. The subject is fascinating and essential in many areas of science and, in particular, wave propagation problems. In this tutorial, we strive to present rigorously and clearly the basic concepts and the tools that are needed to use GFs in applications effectively and with ease. We give many examples to help the readers in understanding the mathematical ideas presented here. The first part of the tutorial is on the basic concepts of GFs. Here we define GFs, their properties and some common operations on them. We define the important concept of generalized differentiation and then give some interesting elementary and advanced examples on Green's functions and wave propagation problems. Here, the analytic power of GFs in applications is demonstrated with ease and elegance. Part 2 of this tutorial is on the diverse applications of generalized derivatives (GDs). Part 3 is on generalized Fourier transformations and some more advanced topics. One goal of writing this tutorial is to convince readers that, because of their powerful operational properties, GFs are essential and useful in engineering and physics, particularly in aeroacoustics and fluid mechanics.
Aeroacoustics of Pipe Systems with Closed Branches
Authors: Tonon Devis , Hirschberg Avraham , Golliard Joachim , Ziada Samir
Published: April 2011

Flow induced pulsations in resonant pipe networks with closed branches are considered in this review paper. These pulsations, observed in many technical applications, have been identified as self-sustained aeroacoustic oscillations driven by the instability of the flow along the closed branches. The fundamental aspects of the flow induced pulsations are discussed, with particular attention to the description of the sound sources. A single mode model for the prediction of the self-sustained oscillations is presented, the “energy balance”. This model consists of the evaluation of the amplitude of each acoustic mode of the system by means of a balance between the acoustic source power and the acoustic power losses. The main components of this prediction method are discussed; these are the evaluation of the acoustic behavior of a pipe network and the modeling of the sound sources and the acoustic losses. Several field and scale model examples of pipe systems displaying self-sustained oscillations are presented, in order to discuss the parameters influencing the aeroacoustic behavior of pipe networks. Finally some counter-measures for the prevention of self-sustained oscillations are reviewed and perspectives for future work are considered.