bluuebunny/crossref_metadata_2025 huggingface dataset:
Qiweibaizhu Decoction Treats Diarrheal Juvenile Rats by Modulating the Gut Microbiota, Short-Chain Fatty Acids, and the Mucus Barrier
Authors: Sun Shaodan , Yang Yang , Lin Xiaojie , Chen Peiwen , Ye Liyan , Zeng Liying , Ye Qina , Yang Xiangna , Ceng Jingtu , Shan Jiayi , Xie Li , Jiang Meirong , Luo Fei , Chen Xiaogang
Published: January 2021

Background. Qiweibaizhu decoction (QBD), a classic Chinese herbal formula, has been widely used for treating diarrhea in infants and children with spleen deficiency syndrome for centuries, but its mechanism of action remains unclear. The gut microbiota, short-chain fatty acids (SCFAs), and intestinal mucus are closely associated with diarrhea. Methods. In this study, the composition of the gut microbiota in diarrheal rats was analyzed by 16S rDNA amplicon sequencing. The concentrations of colon SCFAs were determined using gas chromatography-mass spectrometry (GC-MS). The expression of mucin 2 (MUC2) in the colon was detected by immunofluorescence. Results. Diarrhea significantly changed the diversity and structure of the gut microbiota and disrupted the mucus barrier in juvenile rats. QBD did not significantly change the diversity and structure of the intestinal flora, but it enhanced the increasing tendencies of Verrucomicrobia and Akkermansia and decreased the abundance of Turicibacter (P=0.037) and Flavonifractor (P=0.043). QBD tends to repair the mucus layer and promote MUC2 expression in juvenile rats with diarrhea. Moreover, S. boulardii significantly increased the abundance of Parasutterella (P=0.043). In addition, QBD treatment tends to increase the propionic acid concentration during diarrhea, but its levels of acetic acid, propionic acid, butyric acid, and total SCFAs were lower than those in the S. boulardii group. Conclusion. S. boulardii significantly increased the abundance of Parasutterella, leading to increased production of acetic acid, propionic acid, and butyric acid, consequently leading to alleviation of diarrhea. In comparison, QBD affected diarrhea via regulation of the intestinal flora, especially by increasing the abundance of Verrucomicrobia and Akkermansia, resulting in mucus barrier repair, protection of the intestines, and treatment of diarrhea.
Hand Function as Predictor of Motor Symptom Severity in Individuals with Parkinson’s Disease
Authors: Paz Thiago da Silva Rocha , de Britto Vera Lúcia Santos , Yamaguchi Bruna , Israel Vera Lúcia , Swarowsky Alessandra , Lourenço Correa Clynton
Published: January 2021

Introduction: Parkinson’s disease (PD) leads to deficits in upper limb strength and manual dexterity and consequently resulting in functional impairment. Handgrip strength is correlated with the motor symptom severity of the disease, but there is a gap in the literature about the influence of freezing in PD patients. Objective: The objective is to study the correlation between handgrip strength and motor symptom severity considering the freezing phenomenon and to verify variables that can predict Unified Parkinson’s Disease Rating Scale (UPDRS) III. Methods: This is a multicenter cross-sectional study in PD. 101 patients were divided into 2 groups: freezing of gait (FOG) (n = 51) and nonfreezing (nFOG) (n = 52). Freezing of Gait Questionnaire (FOGQ); UPDRS II and III sections; Hoehn and Yahr (HY) scale; handgrip dynamometry (HD); 9 Hole Peg Test (9-HPT) were assessed. Results: In both groups, HD was correlated to UPDRS III (nFOG: −0.308; FOG: −0.301), UPDRS total (nFOG: −0.379; FOG: −0.368), UPDRS item 23 (nFOG: −0.404; FOG: −0.605), and UPDRS item 24 (nFOG: −0.405; FOG: −0.515). For the correlation to UPDRS II (0.320) and 9-HPT (−0.323), only nFOG group presented significance. For the UPDRS 25 (−0.437), only FOG group presented statistical significance. The UPDRS III can be predicted by 9-HPT, age, and HY in nFOG patients (Adjusted R2 = 0.416). In FOG group, UPDRS III can be predicted by HD, 9-HPT, age, and HY (Adjusted R2 = 0.491). Conclusion: Handgrip strength showed to be predictive of motor impairment only in the FOG group. Our results showed clinical profile differences of motor symptoms considering freezers and nonfreezers with PD.
Diagnostic Approach to Suspected Perihilar Malignancy
Authors: Solonitsyn Evgeny , Dechêne Alexander
Published: January 2021

Background: Cholangiocarcinomas are relatively rare tumors most frequently arising from the epithelium of the hilar bile ducts. The diagnosis is often made in advanced (symptomatic) stages, which accounts for the infavorable prognosis with a 5-year survival of less than 10%. Identification of perihilar cholangiocarcinoma (pCCA) is often challenging because there is no single method offering sufficient diagnostic accuracy. Summary: Most tumors arise in patients without specific risk factors. Clinical symptoms of pCCA are nonspecific and reliable biomarkers are lacking, with carbohydrate antigen 19-9 being the most commonly used tumor marker (but with a low accuracy). Cross-sectional imaging (CT and MRI) is used to identify and map hilar strictures and determine resectability by showing vascular involvement. Endoscopic ultrasound offers additional information on locoregional tumor spread and lymph node involvement. Endoscopic retrograde cholangiography in combination with cholangioscopy gives direct access to and imaging of hilar strictures but it does not always distinguish between pCCA and benign hilar strictures. Tissue acquisition for histological diagnosis is challenging, with frequent sampling errors regardless of the method of biopsy procurement because of the cellular paucity of tumor tissue. Key Messages: In suspected perihilar malignancy, a mosaic of clinical data has to be taken into account. Histological evaluation of (endoscopically harvested) specimens is pivotal to differential diagnosis. Several new techniques to increase diagnostic accuracy are under investigation (biomarkers and genetic testing among others).
Atopische Dermatitis: Dermatologische Systemtherapie in Zeiten der COVID-19-Pandemie
Authors: Philipp Sandra
Published: January 2021

Atopic dermatitis (AD) is a complex disease with elevated risk of respiratory comorbidities. Severely affected patients are often treated with immune-modulating systemic drugs. On March 11th 2020, the World Health Organization declared the 2019 novel coronavirus severe acute respiratory syndrome (SARS-Cov-2) epidemic to be a pandemic. The number of cases worldwide is increasing exponentially and poses a major health threat, especially for those who are elderly, immuno-compromised, or have comorbidities. This also applies to AD patients on systemic immune-modulating treatment. In these days of uncertainty, reallocation of medical resources, curfew, hoarding, and shutdown of normal social life, patients, caregivers and doctors ask questions regarding the continuation of systemic immune-modulating treatment of AD patients. The ETFAD decided to address some of these questions here.
Metabolomic Profiles and Heart Failure Risk in Black Adults: Insights From the Jackson Heart Study
Authors: Tahir Usman A. , Katz Daniel H. , Zhao Tianyi , Ngo Debby , Cruz Daniel E. , Robbins Jeremy M. , Chen Zsu-Zsu , Peterson Bennet , Benson Mark D. , Shi Xu , Dailey Lucas , Andersson Charlotte , Vasan Ramachandran S. , Gao Yan , Shen Changyu , Correa Adolfo , Hall Michael E. , Wang Thomas J. , Clish Clary B. , Wilson James G. , Gerszten Robert E.
Published: January 2021

Background:

Heart failure (HF) is a heterogeneous disease characterized by significant metabolic disturbances; however, the breadth of metabolic dysfunction before the onset of overt disease is not well understood. The purpose of this study was to determine the association of circulating metabolites with incident HF to uncover novel metabolic pathways to disease.

Methods:

We performed targeted plasma metabolomic profiling in a deeply phenotyped group of Black adults from the JHS (Jackson Heart Study; n=2199). We related metabolites associated with incident HF to established etiological mechanisms, including increased left ventricular mass index and incident coronary heart disease. Furthermore, we evaluated differential associations of metabolites with HF with preserved ejection fraction versus HF with reduced ejection fraction.

Results:

Metabolites associated with incident HF included products of posttranscriptional modifications of RNA, as well as polyamine and nitric oxide metabolism. A subset of metabolite-HF associations was independent of well-established HF pathways such as increased left ventricular mass index and incident coronary heart disease and included homoarginine (per 1 SD increase in metabolite level, hazard ratio, 0.77;
P
=1.2×10
−3
), diacetylspermine (hazard ratio, 1.34;
P
=3.4×10
−3
), and uridine (hazard ratio, 0.79;
P
, 3×10
−4
). Furthermore, metabolites involved in pyrimidine metabolism (orotic acid) and collagen turnover (
N
-methylproline) among others were part of a distinct metabolic signature that differentiated individuals with HF with preserved ejection fraction versus HF with reduced ejection fraction.

Conclusions:

The integration of clinical phenotyping with plasma metabolomic profiling uncovered novel metabolic processes in nontraditional disease pathways underlying the heterogeneity of HF development in Black adults.
Defining Shock and Preshock for Mortality Risk Stratification in Cardiac Intensive Care Unit Patients
Authors: Jentzer Jacob C. , Burstein Barry , Van Diepen Sean , Murphy Joseph , Holmes David R. , Bell Malcolm R. , Barsness Gregory W. , Henry Timothy D. , Menon Venu , Rihal Charanjit S. , Naidu Srihari S. , Baran David A.
Published: January 2021

Background:

Previous studies have defined preshock as isolated hypotension or isolated hypoperfusion, whereas shock has been variably defined as hypoperfusion with or without hypotension. We aimed to evaluate the mortality risk associated with hypotension and hypoperfusion at the time of admission in a cardiac intensive care unit population.

Methods:

We analyzed Mayo Clinic cardiac intensive care unit patients admitted between 2007 and 2015. Hypotension was defined as systolic blood pressure <90 mm Hg or mean arterial pressure <60 mm Hg, and hypoperfusion as admission lactate >2 mmol/L, oliguria, or rising creatinine. Associations between hypotension and hypoperfusion with hospital mortality were estimated using multivariable logistic regression.

Results:

Among 10 004 patients with a median age of 69 years, 43.1% had acute coronary syndrome, and 46.1% had heart failure. Isolated hypotension was present in 16.7%, isolated hypoperfusion in 15.3%, and 8.7% had both hypotension and hypoperfusion. Stepwise increases in hospital mortality were observed with hypotension and hypoperfusion compared with neither hypotension nor hypoperfusion (3.3%; all
P
<0.001): isolated hypotension, 9.3% (adjusted odds ratio, 1.7 [95% CI, 1.4–2.2]); isolated hypoperfusion, 17.2% (adjusted odds ratio, 2.3 [95% CI, 1.9–3.0]); both hypotension and hypoperfusion, 33.8% (adjusted odds ratio, 2.8 [95% CI, 2.1–3.6]). Adjusted hospital mortality in patients with isolated hypoperfusion was higher than in patients with isolated hypotension (
P
=0.02) and not significant different from patients with both hypotension and hypoperfusion (
P
=0.18).

Conclusions:

Hypotension and hypoperfusion are both associated with increased mortality in cardiac intensive care unit patients. Hospital mortality is higher with isolated hypoperfusion or concomitant hypotension and hypoperfusion (classic shock). We contend that preshock should refer to isolated hypotension without hypoperfusion, while patients with hypoperfusion can be considered to have shock, irrespective of blood pressure.
Attentional Templates Are Sharpened through Differential Signal Enhancement, Not Differential Allocation of Attention
Authors: Kerzel Dirk , Cong Stanislas Huynh
Published: January 2021

In visual search, the internal representation of the target feature is referred to as the attentional template. The attentional template can be broad or precise depending on the task requirements. In singleton search, the attentional template is broad because the target is the only colored element in the display. In feature search, a precise attentional template is required because the target is in a specific color in an array of varied colors. To measure the precision of the attentional template, we used a cue-target paradigm where cueing benefits decrease when the cue color differs from the target color. Consistent with broad and precise attentional templates, the decrease of cueing effects was stronger in feature than in singleton search. Measurements of ERPs showed that the N2pc elicited by the cue decreased with increasing color difference, suggesting that attention was more strongly captured by cues that were similar to the target. However, the cue-elicited N2pc did not differ between feature and singleton search, making it unlikely to reflect the mechanism underlying attentional template precision. Furthermore, there was no evidence for attentional suppression as there was no cue-elicited PD, even in conditions where the cueing benefit turned into a same-location cost. However, an index of signal enhancement, the contralateral positivity, reflected attention template precision. In general, there was sensory enhancement of the stimulus appearing at the cued location in the search display. With broad attentional templates, any stimulus at the cued location was enhanced, whereas enhancement was restricted to target-matching colors with precise attentional templates.
Dressing like the Great King: Amerindian Perspectives on Persian Fashion in Classical Athens
Authors: Olson S. Douglas
Published: January 2021

This paper examines the phenomenon of individual Athenians adopting elements of Persian clothing, making use of exotic items such as gold and silver drinking vessels, and the like, by comparison to what I argue is a similar sort of contact and exchange involving the European fabric trade and evolving standards of dress and fashion in the Early Modern Atlantic. The ancient literary and archaeological sources discussed document the reaction of a relatively insignificant, marginal people (the Greeks) to the dress practices of a more powerful and arguably far more sophisticated imperial power (the Persians). I suggest that an appreciation of the character and intentions of the much better documented encounter between the English court and Amerindian ambassadors who visited it in the early 18th century provides a potentially useful corrective to the indigenous perspective of our Athenian sources.
Punishment, Stigma and Social Identities in Classical Athens
Authors: Kucharski Janek
Published: January 2021

Taking its cue from modern debates on the expressive function of punishment, this paper discusses the stigmatizing effect of penalties in classical Athens. It focuses on corporal punishment, which was discursively associated in the Athenian public discourse (chiefly comedy and oratory) with slaves and other fringe groups of the citizen community, despite the fact that in reality, with only certain restrictions (e.g. whipping), it was meted out to all social tiers making up the polis-community. Unlike other penalties, those affecting the body were not only public, but not infrequently spectacular. This turned them into versatile devices of stigmatization whereby the punishment itself was no longer considered a one-off event, but a permanent mark branding the person who suffered it as a social outcast.
Superparamagnetic Iron Oxide Nanoparticles Induce Ferroptosis of Human Ovarian Cancer Stem Cells by Weakening Cellular Autophagy
Authors: Huang Yongyi , Lin Jiajia , Xiong Ying , Chen Juan , Du Xiling , Liu Qiang , Liu Te
Published: January 2021

Human ovarian cancer stem cells (HuOCSCs) are the main source of ovarian cancer recurrence, metastasis, and drug resistance. Superparamagnetic iron oxide nanoparticles (SPIONs) are well-known nucleic acid or drug carriers owing to their controllable properties, superior stability, and
easy modification. However, whether SPIONs can inhibit the activity of HuOCSCs by inducing ferroptosis remains unclear. In the present study, we isolated CD44+ /CD133+ HuOCSCs from tumours of four patients with clear cell ovarian cancer and added 0.2 mM SPIONs for mixed
culture. Transmission electron microscopy showed that SPION-treated HuOCSCs contained multiple high-density electron clouds. Prussian blue staining showed high concentrations of iron ions in the cells. In vitro , SPIONs treatment of HuOCSCs inhibited cell proliferation, migration, and soft
agar clone formation, weakened their resistance to multiple chemotherapeutics, and induced cell death. In vivo , SPIONs pretreatment of HuOCSCs significantly reduced their tumour-forming ability and induced angiogenesis in nude mice. Further, SPIONs induced the accumulation of reactive oxygen
species in HuOCSCs and induced oxidative stress. qPCR analysis indicated that SPIONs-treated HuOCSCs had reduced expression of tumour stem cell markers (CD117, NANOG, CD133, and SOX2), cell proliferation factors (KI67, CCND), autophagy-related factors (ATG3, ATG5, MAP1ALC3a, MAP1ALC3b, and
MAP1ALC3c), and certain negative regulators of ferroptosis, while the mRNA expression levels of cell death-related proteins (BAK1 and BID), and certain positive regulators of ferroptosis were significantly increased. Overall, our findings suggest that SPIONs induce oxidative stress and decrease
autophagy activity in ovarian cancer stem cells, activate ferroptosis, and inhibit their proliferation, invasion, drug resistance, and tumorigenic ability.
Confronting Horizontal Ownership Concentration
Authors: Elhauge Einer , Majumdar Sumit K. , Schmalz Martin C.
Published: January 2021

Developments in capital markets have fueled a concentration of horizontal ownership across competing firms, and this has been linked to anticompetitive effects and economic underperformance. The debate about such ownership concentration has proven contentious and controversial. This symposium titled “Common Ownership: Illuminating a Great 21st Century Antitrust Debate” brings together key new works on the topic that confirm, extend, and illuminate the prior empirical findings and policy implications. Among other things, these contributions survey the recent empirical literature, provide new important empirical results about the extent and effect of horizontal ownership, offer a methodological critique, highlight concepts that address core capital market and labor market linkages, and articulate ideas for policy development to tackle emerging contingencies.
Raman Spectroscopy for Adipose Tissue Assessment in Rat Models of Obesity and Type 1 Diabetes
Authors: Donjuán-Loredo Guadalupe , Espinosa-Tanguma Ricardo , León-Bejarano Fabiola , Ramírez-Elías Jordi A. , Salgado-Delgado Roberto , González Francisco J. , Guevara Edgar , Ramírez-Elías Miguel G.
Published: January 2021

Adipose tissue presents structural and functional changes in obesity and type 1 diabetes mellitus (T1DM). In obesity, the size and number of adipocytes and adipokine secretion increases. In T1DM, a loss of adipose tissue suggests changes in the metabolic activity of this tissue. A significant challenge is to find alternative noninvasive methods to evaluate molecular changes in adipose tissue related to obesity and T1DM. Recently, Raman spectroscopy and chemometrics techniques have emerged as a tool for biological tissue analysis. In this work, we propose the use of Raman spectroscopy to characterize spectral differences in adipose tissue from different rat groups (control, obese, and T1DM). The Raman spectra were analyzed using direct band analysis, ratiometric analysis, and chemometric methods (principal component analysis (PCA) and support vector machines (SVMs)). We found that the Raman spectra of obese rats showed significant spectral differences compared to control and diabetic groups related to fatty acids Raman bands. Also, the obese group has a significant decrease in the degree of unsaturation of lipids. The PCA–SVM models showed classification performance ranging from 71.43% to 71.79% accuracy for brown and white adipose tissue samples, respectively. In conclusion, the results demonstrate that Raman spectroscopy can be used as a nondestructive method to assess adipose tissue according to a metabolic condition.
Gas Cell Infrared and Attenuated Total Reflection Infrared Spectroscopic Studies for Organic–Inorganic Interactions in Adsorption of Fulvic Acid on the Goethite Surface Generating Carbon Dioxide
Authors: Nakaya Yuki , Nakashima Satoru , Otsuka Takahiro
Published: January 2021

The generation of carbon dioxide (CO2) from Nordic fulvic acid (FA) solution in the presence of goethite (α-FeOOH) was observed in FA–goethite interaction experiments at 25–80 ℃. CO2 generation processes observed by gas cell infrared (IR) spectroscopy indicated two steps: the zeroth order slower CO2 generation from FA solution commonly occurring in the heating experiments of the FA in the presence and absence of goethite (activation energy: 16–19 kJ mol–1), and the first order faster CO2 generation from FA solution with goethite (activation energy: 14 kJ mol–1). This CO2 generation from FA is possibly related to redox reactions between FA and goethite. In situ attenuated total reflection infrared (ATR-IR) spectroscopic measurements indicated rapid increases with time in IR bands due to COOH and COO– of FA on the goethite surface. These are considered to be due to adsorption of FA on the goethite surface possibly driven by electrostatic attraction between the positively charged goethite surface and negatively charged deprotonated carboxylates (COO–) in FA. Changes in concentration of the FA adsorbed on the goethite surface were well reproduced by the second order reaction model giving an activation energy around 13 kJ mol–1. This process was faster than the CO2 generation and was not its rate-determining step. The CO2 generation from FA solution with goethite is faster than the experimental thermal decoloration of stable structures of Nordic FA in our previous report possibly due to partial degradations of redox-sensitive labile structures in FA.
Governance Gaps in the Sixth Schedule Mechanisms in Meghalaya
Authors: Soreide Kavita N.
Published: January 2021

The legal-political discourse at the time of drafting of independent India’s Constitution resulted in a unique constitutional arrangement guaranteeing a model of self-government through setting up Autonomous District Councils (ADCs) for some of India’s designated tribal communities. This constitutional modality governing the tribal majority regions in India’s North-east is known as the Sixth Schedule. Given the pre-existing tribal institutions, it was implied that ADCs were to act as ‘bridges of governance’ between the state and traditional polity. This article tries to look at the nature of governance and gaps in governance through the lens of ADCs.
Functional dyeable polypropylene fabric development and process parameter optimization Part I: Dyeable modified polypropylene development with process parameter optimization
Authors: Kuo Chung-Feng Jeffrey , Chen Shih Hsiung
Published: January 2021

This study aims to develop dyeable modified polypropylene (PP) granules with disperse dye. The optimal dyeable modified PP granule process used polyester as a mixed copolymer. The purpose was to overcome the excessive difference between the polyester material melting point and PP melting point. The development of a low-melting modified co-polybutylene adipate terephthalate (Co-PBAT) was the key point. After the low-melting modified Co-PBAT was presented, PP and a PP grafting maleic anhydride compatibilizer were made into a composite by dual-screw mixing process. The disperse dye dyeability was reached by the molecular behavior of the Co-PBAT chain segment. The prepared material was applied to explore the thermal properties of modified ester pellets and the functional group was verified by Fourier infrared spectroscopy. In this study, the Taguchi method and principal component analysis were used to optimize the process parameter design of two quality characteristics; namely, the color strength and the polymer melt flow index (MFI). According to the results, the multi-quality optimization of the ester pellets consisted of a modified Co-PBAT melting point of 170°C, the modified Co-PBAT content of 9 wt%, the compatibilizer content of 3 wt%, and the mixing temperature of 205°C. The MFI of the regular PP polymer was 28.1 g/10 min, the color strength was 100 K/S. For the optimal process, the MFI of the PP/Co-PBAT dyeable granules was 37.88 g/10 min, and the color strength was 121.31 K/S. It could be observed that the developed polymer had good circulating workability and color strength.
Urban migrant and refugee solidarity beyond city limits
Authors: Bauder Harald
Published: January 2021

Cities known around the world as sanctuary, solidarity or refuge cities are resisting restrictive national migration and refugee policies and are seeking ways to accommodate migrants and refugees who lack support from the nation state. In this paper I examine urban solidarity approaches in Berlin and Freiburg in Germany, and Zurich in Switzerland. Interviews with key informants reveal that urban solidarity in these cities is not limited to including migrants and refugees living within the city’s boundaries. Rather, urban solidarity reaches beyond municipal boundaries to connect different places and scales in the form of inter-urban solidarity networks and initiatives that aim to enable migrants and refugees who are still abroad to arrive in the city. The complex geographies of urban migrant and refugee solidarity reach far beyond city limits.
Peer mentoring in an extracurricular music class
Authors: Goodrich Andrew
Published: January 2021

The purpose of this study was to explore how high school students mentored beginning fifth-grade students in an extracurricular music class for double-reed instruments. In this study, I investigated the role of peer mentoring with how high school mentors shared their knowledge and experiences, and the role of the teacher in this process. The following questions guided this study: How did the participants share their knowledge and experiences during peer mentoring? How did the teacher serve as a guide for the student participants during peer mentoring? Data collection included observations and interviews during one semester of instruction, and analysis of the data collected involved a system of coding from which two themes developed: mentoring concurrent with director teaching and learning while mentoring. In addition, findings indicated that the participants used knowledge learned from outside sources, they reinforced content presented by the teacher, and they drew on their own musical knowledge during the process. Under the guidance of the teacher, the participants also learned how to share their knowledge that in turn helped elevate their individual levels of musicianship. Implications for music teachers suggest the use of peer mentoring to help create meaningful learning experiences in their classrooms and increase interaction among students.
Professional reflexivity and the paradox of freedom: Negotiating professional boundaries in a Jewish Ultra-Orthodox female music teacher education programme
Authors: Westerlund Heidi , Karlsen Sidsel , Kallio Alexis
Published: January 2021

Embracing an ethos of sharing music and practices across cultural boundaries, the multicultural vision of music (teacher) education has paid scarce attention to the paradox of freedom that arises between such freedoms and the complex politics that frame and constrain teachers’ choices and values. In this article, we explore these demands of professional reflexivity through an instrumental case study of music teacher educators working in an all-female, Ultra-Orthodox Jewish music teacher education programme in Israel. Through a thematic analysis of data generated together with six teacher educators, we illustrate how their context-responsive approaches to developing programme visions engage with processes of censorship and cultural translation, as teachers work to align their professional boundaries within established religious boundaries. This boundary-matching and hybridity required may be seen to result in intense processes of professional reflexivity that raises questions as to how all teacher education programme visions might engage with the moral order of a society and highlights the need for international music teacher education to develop a critical, reflexive awareness of how values shape professional work.
Parkinson patients have a presynaptic serotonergic deficit: A dynamic deep brain stimulation PET study
Authors: Jørgensen Louise M , Henriksen Tove , Mardosiene Skirmante , Keller Sune H , Stenbæk Dea S , Hansen Hanne D , Jespersen Bo , Thomsen Carsten , Weikop Pia , Svarer Claus , Knudsen Gitte M
Published: January 2021

Patients with Parkinson’s disease (PD) often suffer from non-motor symptoms, which may be caused by serotonergic dysfunction. Apart from alleviating the motor symptoms, Deep Brain Stimulation (DBS) in the subthalamic nucleus (STN) may also influence non-motor symptoms. The aim of this study is to investigate how turning DBS off affects the serotonergic system. We here exploit a novel functional PET neuroimaging methodology to evaluate the preservation of serotonergic neurons and capacity to release serotonin. We measured cerebral 5-HT1BR binding in 13 DBS-STN treated PD patients, at baseline and after turning DBS off. Ten age-matched volunteers served as controls. Clinical measures of motor symptoms were assessed under the two conditions and correlated to the PET measures of the static and dynamic integrity of the serotonergic system. PD patients exhibited a significant loss of frontal and parietal 5-HT1BR, and the loss was significantly correlated to motor symptom severity. We saw a corresponding release of serotonin, but only in brain regions with preserved 5-HT1BR, suggesting the presence of a presynaptic serotonergic deficit. Our study demonstrates that DBS-STN dynamically regulates the serotonin system in PD, and that preservation of serotonergic functions may be predictive of DBS-STN effects.
Intrathecal and systemic alterations of L-arginine metabolism in patients after intracerebral hemorrhage
Authors: Mader Marius M , Böger Rainer , Appel Daniel , Schwedhelm Edzard , Haddad Munif , Mohme Malte , Lamszus Katrin , Westphal Manfred , Czorlich Patrick , Hannemann Juliane
Published: January 2021

Alterations in the concentration of nitric oxide (NO) and L-arginine metabolites have been associated with the pathophysiology of different vascular diseases. Here, we describe striking changes in L-arginine metabolism after hemorrhagic stroke. Blood and cerebrospinal fluid (CSF) samples of patients with intracerebral hemorrhage (ICH) and/or intraventricular hemorrhage were collected over a ten-day period. Liquid chromatography-tandem mass spectrometry was used to quantify key substrates and products of L-arginine metabolizing enzymes as well as asymmetric (ADMA) and symmetric dimethylarginine (SDMA). Changes in the plasma were limited to early reductions in L-ornithine, L-lysine, and L-citrulline concentrations. Intrathecally, we observed signs of early NO synthase (NOS) upregulation followed by a decrease back to baseline accompanied by a rise in the level of its endogenous NOS-inhibitor ADMA. SDMA demonstrated increased levels throughout the observation period. For arginase, a pattern of persistently elevated activity was measured and arginine:glycine amidinotransferase (AGAT) appeared to be reduced in its activity at later time points. An early reduction in CSF L-arginine concentration was an independent risk factor for poor outcome. Together, these findings further elucidate pathophysiological mechanisms after ICH potentially involved in secondary brain injury and may reveal novel therapeutic targets.
Analysis of drug resistance and mutation profiles in Mycobacterium tuberculosis isolates in a surveillance site in Beijing, China
Authors: Zhang Jie , Ren Yixuan , Pan Liping , Yi Junli , Guan Tong , Yang Xinyu , Zhang Zongde
Published: January 2021

Objective

This study analyzed drug resistance and mutations profiles in Mycobacterium tuberculosis isolates in a surveillance site in Huairou District, Beijing, China.

Methods

The proportion method was used to assess drug resistance profiles for four first-line and seven second-line anti-tuberculosis (TB) drugs. Molecular line probe assays were used for the rapid detection of resistance to rifampicin (RIF) and isoniazid (INH).

Results

Among 235 strains of M. tuberculosis, 79 (33.6%) isolates were resistant to one or more drugs. The isolates included 18 monoresistant (7.7%), 19 polyresistant (8.1%), 28 RIF-resistant (11.9%), 24 multidrug-resistant (MDR) (10.2%), 7 pre-extensively drug-resistant (XDR, 3.0%), and 2 XDR strains (0.9%). A higher rate of MDR-TB was detected among previously treated patients than among patients with newly diagnosed TB (34.5% vs. 6.8%). The majority (62.5%) of RIF-resistant isolates exhibited a mutation at S531L in the DNA-dependent RNA polymerase gene. Meanwhile, 62.9% of INH-resistant isolates carried a mutation at S315T1 in the katG gene.

Conclusion

Our results confirmed the high rate of drug-resistant TB, especially MDR-TB, in Huairou District, Beijing, China. Therefore, detailed drug testing is crucial in the evaluation of MDR-TB treatment.
Pulmonary Actinomycosis in South Australian Koalas (Phascolarctos cinereus)
Authors: Stephenson Tamsyn , Lee Ken , Griffith Joanna E. , McLelland David J. , Wilkes Anthony , Bird Philip S. , Trott Darren J. , Speight K. Natasha , Hemmatzadeh Farhid , Woolford Lucy
Published: January 2021

Pneumonia has been reported in both free-ranging and captive koalas and a number of causative agents have been described. Between 2016 and 2019, 16 free-ranging and 1 captive koala ( Phascolarctos cinereus) from the Mount Lofty Ranges of South Australia were identified with pyogranulomatous lobar pneumonia, which involved the left caudal lobe in 14/17 (82%) cases. Within lesions, numerous gram-positive or gram-variable, non-acid-fast filamentous bacteria were observed in association with Splendore-Hoeppli phenomenon. Culture yielded growth of anaerobic bacteria, which were unidentifiable by MALDI-TOF-MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry) analysis in 5/5 cases. Sequencing of the bacterial 16S rRNA gene identified a novel Actinomyces species in 4 samples, confirming a diagnosis of pulmonary actinomycosis. Concurrent examination of resin lung casts from healthy koalas suggested greater laminar flow of air to the left caudal lung lobe in koalas. Actinomyces spp. have been reported as commensals of the oral microbiome in other species, and an association with similar pulmonary lesions in other species. Considering the predilection for involvement of the left caudal lung lobe, aspiration is suggested as the likely cause in some cases of pulmonary actinomycosis in koalas. Pulmonary actinomycosis has not been previously described in koalas and further work needs to be undertaken in order to classify this organism within the Actinomyces genus.
Equine Sarcoids—Causes, Molecular Changes, and Clinicopathologic Features: A Review
Authors: Ogłuszka Magdalena , Starzyński Rafał Radosław , Pierzchała Mariusz , Otrocka-Domagała Iwona , Raś Andrzej
Published: January 2021

Equine sarcoid is the most common skin tumor of horses. Clinically, it occurs as a locally invasive, fibroblastic, wart-like lesion of equine skin, which has 6 clinical classes: occult, verrucose, nodular, fibroblastic, mixed, and malignant. Sarcoids may be single but multiple lesions are more frequent. The typical histological feature is increased density of dermal fibroblasts which form interlacing bundles and whorls within the dermis. Lesions are mostly persistent, resist therapy, and tend to recur following treatment. In general, sarcoids are not fatal but their location, size, and progression to the more aggressive form may lead to the withdrawal of a horse from use and serious infringement of their welfare leading to the loss of valuable animals. Bovine papillomavirus (BPV) type 1 and less commonly type 2 contribute to the development of equine sarcoid. The viral genome and proteins are detected in a high percentage of cases. Furthermore, viral oncoprotein activity leads to changes in the fibroblastic tissue similar to changes seen in other types of tumors. Equine sarcoids are characterized by a loss of tumor suppressor activity and changes allowing abnormal formation of the affected tissue, as well as y immune defense abnormalities that weaken the host’s immune response. This impaired immune response to BPV infection appears to be crucial for the development of lesions that do not spontaneously regress, as occurs in BPV-infected cows.
The Shame of Drinking Alcohol While Pregnant: The Production of Avoidance and Ill-Health
Authors: Matebese Sibongile , Macleod Catriona Ida , Tsetse Nontozamo
Published: January 2021

In this article, we examine the operation of shame in the alcohol use habits of pregnant women and the responses of their families and associated institutions. Using a narrative–discursive approach, we interviewed 13 women, living in a low-resource setting in South Africa, who had consumed alcohol while pregnant. Narratives showed how both the act of drinking and “inappropriately” timed pregnancy (early and out of wedlock) were judged to be unacceptable. Women who engaged in these activities were positioned as bad mothers or promiscuous. Their actions were seen as resulting in the suffering of others—the future child, the family, and even the community. These narratives were underpinned by cultural and religious discourses. Women managed the shame accruing to them through avoidance and concealment; families instructed women to self-exclude or distanced themselves from the women’s behavior; and institutions subtly or overtly excluded women. The shaming of these women, and the mechanisms by which such shame was managed, did little to decrease drinking or to increase maternal health and welfare. Overall, this article demonstrates how the shame of drinking alcohol during pregnancy produces avoidance behavior, concealment, and exclusion, which are not constructive in terms of maternal health and well-being. The implications for a feminist narrative approach to drinking during pregnancy are outlined: moving beyond a focus on individual behavior change to locating personal stories within the meta-narratives and social discourses that shape pregnant women’s lives.
Lesions associated with Bartonella taylorii–like bacterium infection in a free-ranging, young-of-the-year raccoon from Prince Edward Island, Canada
Authors: Fenton Heather , McBurney Scott , Elsmo Elizabeth J. , Cleveland Christopher A. , Yabsley Michael J.
Published: January 2021

A young-of-the year, female raccoon was presented with clinical signs of weakness and tremors. The raccoon was euthanized, and autopsy findings included poor body condition, diffuse lymphadenopathy, and pale, firm kidneys with petechial hemorrhages throughout the renal cortex. Histologic lesions included systemic fibrinoid vascular necrosis and severe renal lesions, including lymphoplasmacytic interstitial nephritis and fibrinosuppurative glomerulonephritis. Inflammatory vascular lesions were also present within the uvea, heart, lymph nodes, and the lamina propria of the gastric wall. Ancillary testing was negative for Borrelia burgdorferi, Leptospira sp., Aleutian disease virus, canine distemper virus, feline coronavirus, porcine circovirus 2, and rabies virus. Transmission electron microscopy revealed large numbers of ~1.3 × 0.35 µm bacterial rods surrounded by a trilaminar cell wall located within the glomeruli and associated with aggregates of fibrin and vascular damage. Analysis of partial citrate synthase gene and 16S-23S ribosomal RNA intergenic spacer region sequences from kidney tissue confirmed that the organism was a Bartonella spp. that was related to numerous Bartonella spp. from shrews in Europe. This group formed a sister clade to the genetically diverse Bartonella taylorii group that has been reported from a wide range of Eurasian rodent and flea species.
Effect of the shape of the softening damage law on the predicted tensile fracturing and energy dissipation in textile composites
Authors: Xue Jing , Kirane Kedar
Published: January 2021

The fracturing behavior of fiber-reinforced composites is often modeled using continuum damage mechanics-based approaches which commonly assume a linear softening damage law, i.e. a linearly decreasing stress with increasing strain. The objective of this paper is to assess the suitability of this assumption for composites and to systematically analyze the effect of the assumed shape of the softening damage law at material point, on the predicted fracturing behavior of the structure. The material considered is an epoxy/carbon-fiber twill woven composite. Numerical evaluations are conducted by predicting the strength size effect and progressive accumulation of damage in a structure and comparing with experimental measurements. Damage models with a wide range of shapes of softening laws are considered. These include linear, bilinear, trilinear, exponential, power law and sigmoidal. The effect of introducing a sudden drop in the damage law is also evaluated. All modeling is conducted via the crack band model (CBM), to ensure the mesh objectivity of results. It is found that despite using the right strength and fracture energy as input at the material point level, not all softening law shapes can accurately predict the strength size effect and post-peak softening. Especially the sudden drop feature leads to severe underprediction of strength. Bilinear softening shows the best combination of simplicity and accuracy, especially if formulated via the R-curve approach. Further insights are obtained by assessing the predicted fracture process zone size and fracture energy via the size effect method. Finally structure level predictions are made by considering plug initiated axial crushing of a cylindrical crushcan. The close ties between the shape of the material softening law and predicted process zone, and structural fracturing are revealed, and shown to be an important consideration in formulating damage models.
Internationalizing the Coopetition Construct: Quadratic Effects on Financial Performance Under Different Degrees of Export Intensity and an Export Geographical Scope
Authors: Crick James M. , Crick Dave
Published: January 2021

Although coopetition (simultaneous cooperation and competition) is likely to enhance financial performance if effectively managed, earlier investigations have overlooked the complexities of this relationship. Most notably, understanding the impact of moderating factors can help unpack the complexity of the association between coopetition and performance. Therefore, grounded in resource-based theory and the relational view, this study focuses on the quadratic relationship between coopetition and financial performance under different degrees of export intensity and export geographical scope. Using survey responses from 101 wine producers in New Zealand, the empirical results show that coopetition has a nonlinear (inverted U-shaped) relationship with financial performance. Furthermore, export intensity and export geographical scope positively moderate this quadratic association. As such, for underresourced firms with overseas market potential, decision makers should consider the merits of combining the benefits of coopetition with those from an internationalized business model. This arrangement can help them navigate these potentially paradoxical forces, assuming they engage with trustworthy and complementary rivals in coopetition partnerships.
Tinzaparin Safety in Patients With Cancer and Renal Impairment: A Systematic Review
Authors: Vathiotis I. A. , Syrigos N. K. , Dimakakos E. P.
Published: January 2021

Low-molecular-weight heparins are approved for primary and secondary venous thromboembolism prevention. Tinzaparin is the low-molecular-weight heparin with the highest average molecular weight. The purpose of this systematic review is to provide an update regarding the safety profile of tinzaparin, prescribed either as a prophylactic or as a therapeutic regimen for venous thromboembolism in special populations, including cancer patients and patients with renal impairment. We identified prospective studies up to August 2020 reporting safety outcomes for cancer patients and patients with renal impairment on tinzaparin regimens. In patients with cancer major bleeding rates fluctuated between 0.8% and 7%. Patients on tinzaparin exhibited significantly lower rates of clinically relevant nonmajor bleeding events in comparison with those on vitamin K antagonists. Bioaccumulation of tinzaparin was not correlated with age, body weight or creatinine clearance. Periodic administration of either prophylactic or therapeutic doses of tinzaparin did not result in bioaccumulation, even in patients with severe renal impairment and creatinine clearance < 20 ml/min. Major bleeding rates for non-cancer patients with renal impairment on prophylactic tinzaparin regimens were 0%. Non-cancer patients with renal impairment on therapeutic tinzaparin regimens exhibited major bleeding in 0 to 3.4% of cases; major bleeding rates were higher for cancer patients with renal impairment on therapeutic tinzaparin regimens (4.3 to 10%). Tinzaparin can be used without dose adjustment in patients with severe renal impairment and creatinine clearance > 20 ml/min. Tinzaparin represents a safe choice for special populations at increased risk for thrombosis and bleeding.
Incidence and treatment of feline renal lymphoma: 27 cases
Authors: Williams Ashlyn G , Hohenhaus Ann E , Lamb Kenneth E
Published: January 2021

Objectives

Lymphoma is the most common feline hematopoietic malignancy. Incidence of renal lymphoma has not been reported as a subset of a large population of feline lymphoma cases. Previous studies have reported renal lymphoma as both a singular entity as well as a component of multicentric disease. The clinical presentation, diagnostic evaluation, therapy and outcomes related to renal lymphoma have not been reported since Mooney et al in 1987. This retrospective study aimed to describe the incidence of renal lymphoma, clinical signs, treatment and survival.

Methods

Using a database of cats diagnosed with lymphoma between January 2008 and October 2017, cats with renal lymphoma were selected for further analysis. Cases were retrospectively staged according to Mooney et al (1987) and Gabor et al (1998). Data collected included age, clinical signs, clinicopathologic data, diagnostic imaging findings, lymphoma diagnostic method(s), treatment protocol(s) and survival time. Analyses comparing median survival based on therapy administered, renal lymphoma vs multicentric lymphoma, central nervous system involvement, presence of azotemia, anemia and International Renal Interest Society (IRIS) stage at diagnosis were performed.

Results

From a population of 740 cats with lymphoma, 27 cats had renal lymphoma (incidence, 3.6%), and 14 of those cats had multicentric lymphoma. Fewer stage IV and V cases were identified in this data set compared with Mooney et al; however, not all cats were completely staged. Median survival (range) for cats receiving corticosteroids alone compared with those receiving an L-CHOP (L-asparaginase, vincristine, cyclophosphamide, doxorubicin, prednisolone)-based protocol was 50 days (20–1027 days) in the corticosteroid group and 203 days (44–2364 days) for the L-CHOP group ( P = 0.753) for cats that died secondary to lymphoma.

Conclusions and relevance

Neither clinical stage nor other factors were predictive of survival. Prospective studies are required to determine the optimal chemotherapy protocol.