bluuebunny/crossref_metadata_2025 huggingface dataset:
Polymorphism of PPM1K Gene and The Association Related to Retail Indonesian Sheep Meat Cuts
Authors: Suhendra Muhammad , Ratna Sholatia Harahap  , Kasita Listyarini  , Cece Sumantri  , Cahyo Budiman  , Katrin Roosita  , Gunawan Asep
Published: December 2024

The PPM1K (Protein Phosphatase, Mg2+/Mn2+ Dependent 1K) gene is assumed to associate with retail meat cuts. This study aims to determine the association of the PPM1K gene polymorphisms with retail meat cuts among various breeds of Indonesian sheep. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) approach was used to determine the polymorphisms of the PPM1K gene in 130 Indonesian rams aged 10-12 months with body weights ranging from 20-25 kg. The association study between PPM1K and retail meat cuts was analyzed using General Linear Model (GLM). The research showed that the PPM1K gene was polymorphic, with three genotypes: AA (458 bp), GG (259 bp and 199 bp), and AG (458 bp, 259 bp, and 199 bp). The PPM1K gene polymorphisms were significantly (P<0.05) associated with breast cut, which is the breast muscle and bone. The GG genotype of the PPM1K gene had a higher value on breast lamb. This result implies that the PPM1K gene could be a potential candidate for marker-assisted selection for improving the lamb quality of Indonesian sheep.
The Effect of Sugar Dose and Fermentation Time On The Quality of Sauerkreut as a Source of Probiotic
Authors: Ari Ari Andani Nasution , Trisna Ade , Julianti Elisa
Published: December 2024

This study aims to determine the effect of sugar dosage and fermentation time on the quality of sauerkraut as a source of probiotics. This study used an experimental method in a 3x3 factorial completely randomized design (CRD). The first factor is three treatments of sugar concentration (A), namely A1 (2%), A2 (3.5%), and A3 (5%). The second factor is three treatments of fermentation time (B), namely B1 (5 days), B2 (7 days), and B3 (9 days). The results showed that there was no interaction (P>0.05) between factor A and factor B in the water content, but the water content had a significant effect (P<0.05) separately in factor A and factor B. In total LAB, there was a very significant interaction (P<0.01) between factor A and factor B, and in factor A and factor B. In pH, there was a significant interaction (P<0.05) between factor A and factor B, but a very significant effect (P<0.01) in factor A and factor B. In acid content there was a significant interaction (P<0.05) between factor A and Factor B, but a very significant effect (P<0.01) on factor A and factor B. This study concludes that cabbage processed into sauerkraut can be used as a probiotic supplement. The addition of 3.5% sugar and 7-day fermentation resulted in 91.11% water content, 3.43 pH, 2.93% total acid, and 7.4 x 109 total LAB
Efficacy Of White Turmeric Solution as A Natural Preservative for Eggs: A 12-Hour Soaking Evaluation on Internal Quality
Authors: Krisnaningsih Aju Tjatur Nugroho , Brihandhono Ari , Abrori Yuridlo Jaka
Published: December 2024

The present study investigated the influence of a 12-hour soak in white turmeric solutions at varying concentrations on the internal quality characteristics of eggs laid by purebred chickens, assessing its potential as a natural preservation method. This study utilized 64 one-day-old eggs from Isa Brown hens. This study used a complete randomized design in a lab setting with four treatments and four replications.  Each replicate consisted of 4 eggs. The eggs were soaked in one of the following solutions of white turmeric for the study: P0: 0%, P1: 15%, P2: 30%, and P3: 45%. The variables observed consisted of egg yolk color, egg white index, egg yolk index, and egg pH. The recorded data were subjected analysis of variance. Least significance difference test was applied to identify which mean populations are statistically different. The results showed that soaking eggs using turmeric white solution had a highly significant effect (P<0.01) on egg yolk color, egg white index, egg yolk index, and egg pH. The average yolk color was highest at P2 (7.69±0.13), egg white index at P2 (0.08±0.01), egg yolk index at P2 (0.44±0.01), lowest pH value at P2 at 7.73 and highest at 9.14 at P0. It can be concluded that the internal quality of purebred chicken eggs can be enhanced by soaking them for 12 hours in a solution of 30% white turmeric, which can be used as a natural preservative.
A liberdade religiosa como elemento fundamental ao estado democrático de direito
Authors: Lima Adriano Sousa , Dutra Lincoln Zub
Published: December 2024

Este artigo aborda a liberdade religiosa como elemento fundamental ao Estado Democrático de Direito, haja vista a imperiosa importância e discussão inerente ao tema, decorrente da autonomia de vontade correlacionada com a fé religiosa. A importância dada ao presente tema, atualmente, ganha cada vez mais atenção, tanto dos operadores do direito, quanto da própria sociedade, haja vista que guardam relação direta com os direitos fundamentais preconizados pela Constituição da República e, assim, com o Estado Democrático de Direito. A relevância quanto ao tema no âmbito das Ciências das Religiões e Teologia se demonstra de suma importância, vez que dia após dia vem ganhado corpo no Direito e, assim, em nosso Estado Democrático de Direito, tendências de compreensão e, inclusive, novas regulamentações positivadas que vão de encontro com nossos princípios e ideais democráticos, fortalecendo a necessidade do debate interdisciplinar. Sendo assim, a partir de um sólido referencial teórico, objetiva-se apresentar a liberdade religiosa como um direito fundamental constitutivo para o Estado Democrático. Nesse sentido, a liberdade religiosa é também um elemento importante para compreender o pluralismo cultural e religioso da sociedade brasileira. Assim, os autores concluem: num país cuja diversidade religiosa é latente, o respeito à liberdade de crença é absolutamente necessário e fundamental no fortalecimento da democracia.
Horizontes epistêmicos da educação antirracista: uma ressignificação ontológica do fazer, do ser e do pensar. Perspectivas anticolonialistas
Authors: Ferreira Alves Adeir
Published: December 2024

As mais antigas civilizações têm suas próprias cosmogonias (mitos fundadores acerca do mundo), e muitas delas também possuem suas escatologias (narrativas que versam sobre o fim do mundo). Destaco neste estudo o colonialismo como propulsor da narrativa escatológica judaico-cristã – instrumentalizada também como poder de dominação em diversos aspectos (racial, de gênero, sexual, de classe, cultural), especialmente epistêmicos.  Além destes fatores estruturantes, o logos ocidental se modernizou com outros conteúdos e novas soberanas gestões administrativas de territórios marginais (inspirando o cosmopolitismo e o imperialismo, especialmente o norte-americano). Caudatário da narrativa escatológica ocidental, o Antropoceno reúne diferentes fundamentos do colonialismo, cujo resultado preconiza um modo cultural homogêneo hegemônico de habitar e de desabitar o mundo – gerando, com efeito, uma profusão de assimetrias. Quase desterritorializada, a geografia das fronteiras contemporâneas parece anunciar como ontologia imanente não mais divisas territoriais do globo terrestre, e sim quem pode viver e quem deve morrer.
Guerra Fria, Religião e fundamentalismo religioso
Authors: Mariano Rubem
Published: December 2024

Durante a Guerra Fria (entre Estados Unidos - EUA e União Soviética - URSS, entre 1947 e 1991), a influência religiosa dos EUA no Brasil e em outros países da América Latina foi evidente, com investimentos em ações de missionários e entidades religiosas fundamentalistas que apoiavam as agendas políticas e diplomáticas dos EUA. A disputa entre líderes religiosos do protestantismo brasileiro em relação à filiação ao Conselho Mundial de Igrejas – CMI em 1964 refletiu o embate entre antiecumenismo e ecumenismo, assim como anticomunismo e comunismo. Enquanto alguns defendiam a não filiação, alegando inclinações socialistas e comunistas do CMI, outros argumentavam a favor da existência de espaço para diferentes sistemas políticos e econômicos dentro da comunidade cristã. O presente artigo mostra a necessidade de estudar a relação entre religião e Guerra Fria para compreender melhor as dinâmicas políticas e sociais desse período e a importância da presença de como se constituiu e se desenvolveu o fundamentalista religioso como pensamento importante nesse contexto.
 
Palavras-chaves: Guerra Fria; Religião; Fundamentalismo religioso; Catolicismo e Protestantismo.
A TFP contra o Papa Francisco: uma análise da reação católica ultraconservadora ao Sínodo para a Amazônia
Authors: Cloclet da Silva Ana Rosa , De Jesus Azevedo Rodrigo
Published: December 2024

Dentre as iniciativas do Papa Francisco que vão ao encontro do seu ideal eclesial, o Sínodo para a Amazônia, convocado em outubro de 2017, encontra-se entre aquelas mais diretamente relacionadas à realidade brasileira. Este teve como foco a situação da Igreja católica na região amazônica, envolvendo questões ligadas ao meio ambiente e aos povos originários no Brasil. O teor dos temas discutidos aprofundou as controvérsias e cisões que acompanham o magistério de Francisco, tanto no âmbito intraeclesial, quanto naquele externo à Igreja. É neste sentido que este artigo busca analisar a reação político-religiosa da TFP (Tradição, Família e Propriedade), grupo católico ultraconservador, ao Sínodo para a Amazônia. Assim, objetiva-se identificar e analisar quais dos temas tratados no Sínodo foram priorizados pela TFP e qual o teor de sua crítica a eles dirigida. Em termos metodológicos, o trabalho se pauta na instrumentalização do método da Análise de Discurso sobre os documentos sinodais e sobre os conteúdos postados nos principais veículos de divulgação da TFP.
Brazilian Physiotherapists’ Perceptions on Spirituality, Religiosity and Health: A Cross-sectional Study
Authors: Reis Alves Katia , Barbosa Góes Ana Lúcia , Santana Genildes , Cordeiro de Souza Anselmo , Nunes Sá Katia
Published: December 2024

Foi realizado um estudo transversal quali-quantitativo para traçar o perfil do fisioterapeuta brasileiro quanto à sua própria espiritualidade e analisar sua percepção sobre o efeito da espiritualidade ou da religiosidade na recuperação do paciente. Um questionário autoaplicável do Google Forms, incluindo a Escala de Bem-Estar Espiritual e o Índice de Religião da Duke University, além de duas questões abertas foi aplicado em 374 fisioterapeutas. As mulheres apresentaram maior religiosidade não organizacional e intrínseca, enquanto os homens apresentaram maior religiosidade intrínseca (p=0,005). Aqueles que viviam com companheiros também apresentaram maior nível de espiritualidade (p<0,005). Os fisioterapeutas brasileiros acreditam que o nível de espiritualidade interfere nos resultados do tratamento, muitas vezes incentivam a fé, a esperança, o otimismo, as práticas meditativas, as músicas relaxantes e os exercícios respiratórios e a busca de significado nos problemas de saúde.
A espiritualidade cotidiana frente a fragilidade da vida
Authors: Adriano Odete Liber de Almeida
Published: December 2024

A espiritualidade é uma forma de construção de sentido para a vida que ultrapassa as confissões religiosas e não depende de lugar, tempo ou códigos. Como, ao longo da vida as pessoas se deparam com muitas dificuldades, sendo que algumas delas podem levar ao adoecimento, a espiritualidade apresenta vários recursos para o enfrentamento de situações que muitas vezes são inevitáveis. O ato de envelhecer, por exemplo, especialmente quando acompanhado de doenças, traz sofrimento para a maioria das pessoas e, como tal, provoca angústias, tristeza, dor, especialmente em situações que envolvem a demência, mais comum em idosos, com sua incidência e prevalência aumentando significativamente com a idade. Esse artigo procura demonstrar como a espiritualidade oferece um referencial positivo, repleto de esperança para o enfrentamento da doença. A espiritualidade é benéfica para a saúde e qualidade de vida. É também fonte de força e amparo para lidar com a realidade de familiares cuidadores de idosos com demências, bem como inspiração para sentimentos de gratidão e satisfação.
Understanding the Interplay between African Trypanosomiasis and Sleep Disorders: A Comprehensive Review
Authors: Pitton Rissardo Jamir , Byroju Vishnu Vardhan , Fornari Caprara Ana Leticia
Published: December 2024

African trypanosomiasis, or sleeping sickness, is a neglected tropical disease caused by Trypanosoma brucei parasites and transmitted by tsetse flies. Traditionally recognized for its neurological manifestations, including meningoencephalitis and cognitive impairment, sleeping sickness also profoundly affects sleep patterns and quality of life among affected individuals. This comprehensive review explores the intricate interplay between African trypanosomiasis and sleep disorders, examining the disease's epidemiology, pathogenesis, clinical manifestations, and diagnosis. We delve into the mechanisms underlying sleep disturbances in sleeping sickness, including disruptions of circadian rhythms, neurotransmitter imbalances, immune system dysregulation, and neurological complications. Furthermore, we discuss the challenges and opportunities in diagnosing, treating, and managing sleep disorders in the context of African trypanosomiasis, emphasizing the importance of integrated approaches to healthcare delivery, community engagement, and interdisciplinary research collaboration. By synthesizing current evidence from clinical studies and experimental research, this review provides insights into the bidirectional relationship between African trypanosomiasis and sleep disorders, identifies gaps in knowledge, and proposes future research directions to improve clinical outcomes for individuals affected by sleeping sickness.
Technology Use Momentums by Human: The Potential Outcomes of Integrating Artificial
Authors: Mohammad Reda Abdelfattah , Abdelkhair Farah Yasin , Bindawas Abdullah Mobarak , Abubaker Attico Yasir Tageldin , Abdu Edris Ahmed Edris , Hamed Ali Abdelgfar Abdalla , Al-Qubayi Ahmad
Published: December 2024

This study has a novel conceptual framework and uses SPSS version 23 and AMOS version 23 for analysis. These findings reveal that the use of AI in HR processes has, in fact, a positive significant effect on Employee Engagement and Retention, Data-Driven Decision Making, Smarter Recruitment, and Enhancing Performance Management but has no effect on Personalized Learning and Development. The novelty of this contribution lies in its focus on specific outcomes of the adoption of AI technology in managing the HRM process. This study fills the conceptual gap in the literature on HRM through the critical analysis of AI functions in the human resources process, focusing on the following critical outcome variables: employee engagement and retention, data-driven decision making, smarter recruitment, enhancing performance management, personalized learning, and development-the interrelations between HR artificial intelligence and these outcome variables.
Polyamide Membranes Coated with
Nano-Engineered Metal Oxides via RF Sputtering for Advanced Seawater Desalination
Authors: Vargas Catalina , Palacio Daniel , Ramírez Jesús , Pérez Eduardo , Solis-Pomar Francisco , Fundara-Cruz Abel , Bórquez Rodrigo , Jaramillo Andres , Pino* Luis , Melendrez Manuel
Published: December 2024

The most commonly used membranes are of the "thin film composite" (TFC) type, consisting of three layers of polymers. Membrane modification has been explored through two main approaches: optimizing the polymer before fabrication or modifying the surface after fabrication. This study focuses on membrane modification by depositing ultrathin films of zinc oxide (ZnO) using the sputtering process. The impact on permeability and monovalent ion rejection is examined. The results show that an increase in ZnO deposition time leads to a reduction in permeate flux density, albeit with an improvement in monovalent ion rejection. The Spielger-Kedem model is demonstrated to effectively predict the behavior of these membranes. Collectively, the findings suggest that membrane modification with ZnO through sputtering can offer significant advantages in water purification applications, albeit with considerations regarding reduced permeate flux density.
Non-Communicable Diseases: New Potential Therapeutic Application of n-3 Polyunsaturated Fatty Acids, Specialized Pro-Resolving Mediators and Prebiotics/Probiotics
Authors: Amedei Amedeo , Lamminpää Ingrid , Parolini Cinzia
Published: December 2024

There is overwhelming evidence of the anti-inflammatory effects of n-3 polyunsaturated fatty acids (n-3 PUFAs) and probiotics/prebiotics in different chronic non-communicable diseases (NCDs), such as metabolic syndrome, type 2 diabetes, hypertension, cardiovascular disease (CVD), non-infectious respiratory disease, chronic kidney disease, neurodegenerative and autoimmune diseases (i.e., rheumatoid arthritis), and various types of cancers. This review aims at describing and analysing the interplay among marine n-3 (PUFAs), gut microbiome and CVD. Furthermore, we discuss the beneficial role of marine n-3 PUFAs and/or probiotics/prebiotics in the treatment of CVD.
Temporal and regional analysis of Hospital Admission Au-thorizations (AIH) for treatment of facial fractures in the state of Paraíba in the last 10 years (2014-2024)
Authors: Santana Ilan Hudson Gomes de , Viana Mayara Rebeca Martins , Ferreira Anderson Jara , Guimarães Camila Coêlho , Zevallos Erick Andres Alpaca , Schwerz Paola de Cassia Spessato , Félix Karolayne Dutra , Henriques Maria Eduarda Batista , Macêdo Felipe Germoglio Cardoso , Ribeiro Eduardo Dias
Published: December 2024

Introduction: External fractures, often the result of traffic accidents and interpersonal violence, represent a public health problem that requires specialized treatment and hospital infrastructure. In Paraíba, the demand for Hospital Admission Authorizations (AIH) for these injuries has increased over the years, concentrated in large hospitals. This study aims to analyze this demand between 2014 and 2024, providing subsidies for the planning and distribution of health resources. Materials and Methods: Using AIH data from the DATASUS system, we analyzed types of industrial fractures in state hospitals, including number of approvals, average length of hospital stay and associated deaths. The methodology involves descriptive analysis of the evolution of AIHs and the distribution between different hospital doors. Results and Discussion: The data show a significant increase in AIH over the period, with a greater concentration in referral hospitals, such as Dom Luiz Gonzaga Fernandes Hospital and Senador Humberto Lucena Hospital, which handle most of the complex cases. The mortality rate was low, but the rate of hospitalization varied between hospitals, reflecting the complexity of the cases. The results indicate the need to decentralize care and strengthen regional hospitals in order to improve access and reduce the overload on large centers. Conclusion: The study highlights a growing demand for treatment of bone fractures and the importance of strategic planning to strengthen regional hospitals and implement prevention policies, ensuring more equitable and efficient care for the population affected by facial trauma.
Spatial Arrangement of the Potential anti-HIV Drug, 1-[2-(2-Benzoylphenoxy)ethyl]-6-methyluracil, in Crystal and Ligand-Receptor Complex
Authors: Romanenko Alexander , Vologzhanina Anna , Stash Adam , Peresypkina Eugenia , Virovets Alexander , Korlyukov Alexander
Published: December 2024

Understanding of binding between antiretroviral agents and biomacromolecules is important for investigation of processes that proceed during therapy. The conformational stability, binding affinity and energies of interactions in the crystal, ligand-nucleobase pairs and ligand–receptor complexes were studied for 1-[2-(2-benzoylphenoxy)ethyl]-6-methyluracil (VMU-2012-05), a potential anti-HIV drug. The x-ray structure of the crystalline VMU-2012-05 was solved experimentally, and the analysis of intermolecular interactions has been performed by means of Quantum Theory “Atoms in Molecules” (QTAIM). Quantum chemical study of the isolated molecule revealed five stable conformations, their energies differ up to 14.3 kJ/mol, four of them can be described as folded and one as extended or linear. In the crystal, a folded conformation of VMU-2012-05 significantly differs from stable ones for an isolated molecule. Intermolecular interactions in the model complexes of VMU-2012-05 with nucleobases and the HIV-1 reverse transcriptase caused stabilization of the least favorable folded conformation for the molecule. QTAIM and NCI study revealed that the dimers between VMU-2012-05 and the nucleobases are assembled mainly via C-H...π and staking interactions, while in the crystal and the complex with the HIV-1 reverse transcriptase VMU-2012-05 readily forms strong hydrogen bonds. The number of potential acceptors of H-bonding in VMU-2012-05 molecule exceeds the number of donors (1 and 5) that limits the number of hydrogen bonds experimentally observed in the crystal. The number of H-bonds in the model binding pocket with extra donors and acceptors is higher, nevertheless, the energy which goes to N-H…O bonds in the crystal is nearly the same as in the binding pocket (-58.9 and -63.6 kJ/mol, respectively). However, value of the lattice energy is by 37.9 kJ/mol larger than the total energy of the intermolecular interactions with the HIV-1 receptor (-214.6 and -245.2 kJ/mol) due to additional hydrophobic interactions. These results indicate the role of weak van der Waals interactions in stabilization of VMU-2012-05 associates with nucleobases and HIV-1 reverse transcriptase.
Chemo-Mineralogical Changes of Six European Monumental Stones Caused by Cyclic Isothermal Treatment at 600 °C
Authors: Urbanek Matea , Wriessnig Karin , Artner Werner , Pintér Farkas , Ottner Franz
Published: December 2024

This experimental study analyses the extent of chemo-mineralogical changes that occur when a building stone encounters a cycling isothermal treatment at 600 °C. Therefore, four carbonate and two silicate European building stones have been analysed in their fresh quarried and thermally treated conditions by means of colour measurements, in-situ X-ray diffraction (XRD) and optical microscopy. Furthermore, powdered samples have been characterised by Fourier-transform infra-red spectroscopy, simultaneous thermal analysis and cycling thermogravimetry (TG). In-situ XRD spectra reveal a surface limited phase transformation of solid calcite and dolomite at isothermal conditions during the first 10 min at 600 °C and 500 °C, respectively. The onset of thermal de-composition and extend of phase transformation is governed by the microstructure of the solid samples. Inter- and intragranular micro cracks are induced in varying degrees and their incidence depends likewise on the stone’s microstructure. Discolouration indicates a transformation of minor elements across the entire analysed sample volumes. Kaolinite is preserved even after three hours of thermal treatment at its dehydroxylation temperature due to its sheltering in confined pore spaces. Mass loss is more pronounced when cyclic treatment is employed as compared to a nonperiodic treatment, as determined by TG analysis performed at same time intervals.
Polyphenolic Compounds in Fabaceous with Antidiabetic Potential
Authors: Guerrero-Becerra Lucia , Morimoto Sumiko , Arellano-Ordoñez Estefania , Morales-Miranda Angelica , Guevara-Gonzalez Ramon G. , Feregrino-Pérez Ana Angélica , Lomas-Soria Consuelo
Published: December 2024

Diabetes mellitus is a chronic non-communicable disease with increasing prevalence in Latin America and worldwide, impacting various social and economic areas. It causes numerous complications for those affected. Current treatments for diabetes include oral hypoglycemic drugs, which can lead to adverse effects and health complications. Other natural alternatives for DM treatment have been studied as adjunct therapies to reduce or eliminate the need for antidiabetic medications. Several natural supplements may offer an alternative to improve the quality of life for patients with DM and in nutraceutical applications. Due to their phenolic compound content, some leguminous substances have been proposed for these alternatives. Phenolic compounds, with their high antioxidant activity, have shown promising potential in insulin synthesis, secretion, and the functionality of the endocrine pancreas. This review provides valuable information on various leguminous plants with anti-diabetic properties, including antioxidant, hypoglycemic, anti-fat-induced damage, and anti-apoptotic properties in vitro and in vivo, attributed to their high content of phenolic compounds in seeds. Natural products with antidiabetic potential and pharmacological treatments improve diabetes management by offering more effective and complementary alternatives. To integrate these herbal remedies into modern medicine, further research on phenolic compound type, doses, efficacy, and safety in the human population.
Genomic Profiling Reveals Molecular Heterogeneity in Patients with Richter Transformation (RT) and Chronic Lymphocytic Leukemia (CLL)
Authors: Tian Shulan , Wang Hanyin , Parikh Sameer A. , Liu Yuanhang , Jin-Lee Helen , Jessen Erik , Klee Eric W. , Wang Yucai , Leng Fan , Shi Min , Zepeda-Mendoza Cinthya , Kenderian Saad J. , Baughn Linda B. , VanDyke Daniel L. , Hampel Paul J. , Neil Kay E. , Esteban Braggio , Slager Susan L. , Yan Huihuang , Ding Wei
Published: December 2024

Richter transformation (RT) represents the development of an aggressive lymphoma in chronic lymphocytic leukemia (CLL). Patients with RT and relapsed CLL have poor outcomes. Yet, the extent of molecular differences between the two entities has not been fully explored. In this pilot study, we conducted RNA-seq and targeted panel sequencing of nodal tissues from 12 patients, including seven with RT and five with CLL. Analysis of RNA-seq data revealed two major clusters, with five RT in cluster C1 and the remaining two RT and all five CLL in C2. Within C2, one of the CLL ultimately developed RT; it showed more similarity to the two RT than to the other CLL in expression profile, suggesting the presence of expression signature for RT prior to the clinical diagnosis. In addition, differentially expressed genes, the majority of which showed higher expression in C1 relative to C2, were enriched in pathways known to be important for CLL pathogenesis or transformation. Deconvolution of the bulk RNA-seq data revealed major differences in cellular composition between the two clusters, notably tumor B cells, macrophages M1, and CD8+ T cells. Furthermore, by targeted sequencing, we identified 51 genes that carried recurrent copy number alterations (CNAs) preferentially occurring in either cluster. Over 80% of these CNAs occurred in C2, mainly gains of 17q12q25 in CLL. Patients in C1 had shorter overall survival (median 11 months) compared to those in C2 (median 36 months). Together, our findings highlight noticeable differences in transcriptomic and genomic alterations between CLL versus RT.
Melatonin as a Potential Antiarrhythmic Drug: Myocardial Ischemia and Beyond
Authors: Azarov Jan , Durkina Aleksandra , Bernikova Olesya , Gonotkov Mikhail , Tsvetkova Alena , Mikhaleva Natalia , Ovechkin Alexey , Vaykshnorayte Marina , Sedova Ksenia
Published: December 2024

Life-threatening cardiac arrhythmias such as ventricular tachycardia and/or fibrillation often complicate myocardial ischemia and constitute a cause for sudden cardiac death. Development of strategies for the prevention of such arrhythmias present an important research problem. The use of classical antiarrhythmic drugs is related to significant risk due to their aggressive action and probable proarrhythmic side-effects. Such risks are generally affordable in urgent situations, but unacceptable for preventive use in large patient populations. It poses the necessity to develop a safe and effective medication to be applied for arrhythmia prophylaxis and treatment. Melatonin has been proved to be safe for use in humans even in relatively high doses. It has been reported to confer versatile cardioprotective effects, which include antiarrhythmic action. Traditionally, the antiarrhythmic effect is considered as a manifestation of the general protective action of melatonin, based largely on its antioxidative properties. In this review, we strive to provide evidence of specific electrophysiological mechanisms brought about by melatonin application. Here, we discuss the pathways by which melatonin targets ion channels and causes changes in the cellular and tissue properties relevant to arrhythmogenesis.The obtained data suggest that melatonin can modify a number of electrophysiological characteristics of a cardiomyocyte and myocardium. However, the changes in conduction velocity appear to be the major effect functionally relevant for arrhythmogenesis. The most probable mechanisms of the melatonin-related conduction enhancement are discussed. These mechanisms differ in acute and chronic applications of melatonin and related to the increase of inward rectifier potassium current maintaining the resting membrane potential and the upregulation of sodium channel protein expression, respectively.The novel melatonin-related mechanism regulating myocardial conduction velocity might be considered for potential use in different contexts that include arrhythmia risks from myocardial ischemia to inherited channelopathies. The functional relevance of this effect warrants its further exploration concerning cellular mechanisms, efficiency and limitations.Key words: melatonin, arrhythmias, ischemia, reperfusion, conduction velocity, dispersion of repolarization, ionic currents, oxidative stress
Construction and Vibration Analysis of a Sleeve Type Isolator
Authors: Song Yuchao , Dong Wenyu , Zhuang Lei , Yang Yanxin , Li Fangming , Li Guobin , Liao Jianbin
Published: December 2024

For application of non-metallic material in efficient vibration isolation, the polymer materials are used to construct a ST (sleeve type) isolator. The outside parts of ST isolator are made of PLA material, and the inside parts of ST isolator are made of TPU material. The polymer material model is linear elastic and isotropic. The numerical model of the ST isolator is calculated by using the finite element method. The static analysis and vibration isolation performance are analyzed. The numerical results show that when the ST isolator is under a force of 20000 N in vertical direction, the maximun deformation is 0.80 mm and the maximum stress is 2.68×107 Pa. When under a force of 5000 N in horizontal direction, the maximun deformation is 1.44 mm and the maximum stress is 3.70×107 Pa. The natural frequency of ST isolator is higher than 1800 Hz, and the fixed frequency of ST isolator is higher than 560 Hz. The harmonic analysis and transient analysis results show that the ST isolator has an efficient vibration isolation performance, especially when the ST isolator is fixed on a rigid foundation. This 3D printed ST isolator can be used in next engineering application.
Nanoemulgel Formulation of Marjoram (Origanum majorana L.) Essential Oil with Potential as Anti-Inflammatory and Anti-Hyperuricemia
Authors: Purwanto Ungsari Rizki Eka , Syukur Mighfar , Pebriani Tris Harni , Nursofiatun Anissa
Published: December 2024

The prevalence of hyperuricemia has increased in several countries, including Indonesia. Uric acid will continuously be deposited in the form of crystals in the joint cavity, which can cause inflammation. One natural ingredient potentially treating this problem is marjoram essential oil (Origanum majorana L.). Marjoram essential oil (MEO) contains various terpene compounds that have the potential to reduce pro-inflammatory mediators and uric acid levels. Terpene compounds are known to be able to be delivered through the skin, but their penetration levels are still very limited. The development of pharmaceutical preparations such as nanoemulgel is needed to enhance the penetration of the active components in marjoram essential oil. This research aimed to optimize the components that make up nanoemulgel, namely polysorbate 80 surfactants and PEG 400 co-surfactant, to produce a nanoemulgel preparation of marjoram essential oil with optimum physical characteristics and potential as an anti-inflammatory and anti-hyperuricemia in the rat. The optimum composition of Polysorbate 80 surfactant and PEG 400 co-surfactant is 9.958: 6.042. It resulted in the optimal formula for MEO 1% nanoemulsion, which had an average globule size of 22.94 nm with a polydispersity index of 0.65 and MEO 2% nanoemulsion: 142.4 nm with a polydispersity index of 0.91. MEO nanoemulsion could be dispersed into a gel base and become an MEO nanoemulgel, which has inhibitory power against xanthine oxidase, whereas 2% MEO nanoemulgel has an IC 50 of 25.30+2.57 ppm and has greater anti-inflammatory power than 1% MEO nanoemulgel. 
Formulasi, Uji Stabilitas Fisik, dan Efektifitas Sirup Antelmentik Ekstrak Bawang Putih (Allium sativum L.) Terhadap Cacing (Ascaris suum, Goeze)
Authors: Zainal Tuti Handayani , Aksa Rahmad , Utami Yuri Pratiwi , Heldawati Heldawati
Published: December 2024

Umbi yang dapat digunakan untuk infeksi cacingan adalah bawang putih (Allium sativum L.). Kandungan bawang putih yaitu senyawa flavonoid dan saponin berkhasiat sebagai anthelmintik. Tujuan penelitian ini untuk mengetahui efektifitas ekstrak bawang putih sebagai antelmintik terhadap cacing gelang Ascaris suum, Goeze. serta memperoleh formula sirup yang stabil secara fisik. Penelitian ini meliputi persiapan hewan uji, persiapan sampel, pengolahan sampel, proses ekstraksi dengan metode maserasi, skrining fitokimia, uji bebas etanol, uji aktivitas antelmintik ekstrak bawang putih (Allium sativum L.), pembuatan sirup, dan evaluasi. Penelitian ini menggunakan 3 kelompok perlakuan yaitu, larutan NaCl 0.9% sebagai kontrol negatif, pirantel pamoat 1% (Combantrin®) sebagai kontrol positif, dan ekstrak bawang putih (Allium sativum L.) variasi konsentrasi 20% b/v, 25% b/v, dan 30% b/v. Hewan uji untuk setiap perlakuan adalah 6 ekor. Pengamatan kematian cacing dilakukan setiap jam dan selama 48 jam, Hasil perhitungan diperoleh nilai LC50 33,88% dan LC90 93,33% dan waktu kematian 100% cacing adalah 28 jam. Konsentrasi ekstrak yang digunakan dalam pembuatan formulasi yaitu sebesar 30%, dengan variasi konsentrasi sorbitol sebesar 20%, 25% dan 30%.   Formulasi sediaan sirup ekstrak bawang putih yang baik yaitu F3 dengan konsentrasi sorbitol 30% dengan hasil uji organoleptik berwarna coklat kehitaman, rasa manis, aroma strawberry, homogen dan jernih.  Evaluasi fisik pH 5,05, bobot jenis 1,23 g/mL, waktu tuang 2,93, volume terpindahkan 96% dan uji viskositas 8,40 cps. Hasil penelitian menunjukkan bahwa ekstrak bawang putih dapat dibuat sirup antelmintik dan efektif terhadap cacing Ascaris suum, Goeze.  
Autentikasi Minyak Biji Jagung Zea mays L.: Pengaruh Metode Ekstraksi dan Analisis Komposisi Menggunakan Metode Gas Cromatoghraphy-Mass Spectrometry (GC-MS)
Authors: Fadli Justitia Cahyani , Guntarti Any , Salamah Nina
Published: December 2024

Minyak jagung mengandung protein dan asam lemak yang melimpah, sehingga diperlukan metode dan kondisi ekstraksi yang tepat untuk menghasilka kualitas minyak berkualitas tinggi. Minyak dapat diperoleh dengan cara pemanasan menggunakan alat Soxhlet maupun Reflux dan akan terisolasi dengan pelarut non polar. Penelitian ini bertujuan untuk mengoptimalkan ekstraksi minyak jagung manis, membandingkan kualitas minyak jagung dari dua metode isolasi serta menganalisis komposisi asam lemak menggunakan GC-MS. Minyak jagung manis yang dihasilkan dari dua metode isolasi Reflux dan Soxhlet dibandingkan dengan parameter SNI dan diidentifikasi komposisi asam lemaknya menggunakan instrumen GC-MS. Parameter kualitas minyak biji jagung manis yang dihasilkan dari metode Soxhlet dan Reflux, memiliki karakteristik warna, bau, indeks bias, kadar air, bilangan asam, bilangan peroksida, dan bilangan iod yang memenuhi persyaratan SNI. Hasil analisis GC-MS menunjukkan metode Reflux menghasilkan 3 kandungan senyawa yaitu, asam palmiat (31,37%), linoleoyl chloride (67,15%), dan asam ftalat (1,48%), sedangkan metode Soxhlet menghasilkan 2 kandungan senyawa, yaitu asam palmiat (32,28%) dan asam linoleat (66,89%). Hasil penelitian menunjukkan bahwa variasi metode ekstraksi minyak biji jagung memengaruhi kualitas minyak serta kandungan asam lemak yang dihasilkan. 
Анализ динамики эпидемического процесса COVID-19 в мире за неделю с 07.12.2024 г. по 13.12.2024 г.
Authors: Зимирова Анастасия , Поспелов Михаил , Иванова Александра , Дмитриева Людмила , Чумачкова Елена , Карнаухов Игорь , Щербакова Светлана , Кутырев Владимир
Published: December 2024

В обзоре представлен анализ эпидемиологической ситуации в странах мира по COVID-19 за неделю с 07.12.2024 г. по 13.12.2024 г.
Discerning coastal-process signatures and architectural styles in tidal barforms from the deltaic western Paleozoic Gondwana shoreline: new insights about the Balcarce Formation, Argentina
Authors: Olivo Mariana S. , Isla Manuel F. , Moyano-Paz Damián , López Luciano , Percudani Lorena B. , Zuazo Jerónimo J. , Gómez-Peral Lucía E. , Poiré Daniel G.
Published: December 2024

Sedimentological contrasts between coastal to shelf tidal barforms on ancient shorelines, particularly in tidally affected deltas, are relatively poorly understood. A comprehensive analysis of facies distribution and architectural attributes, complemented by virtual outcrop models (VOMs), has unveiled two different tidal barform archetypes in a sand-rich, tide-dominated deltaic succession in the lower Paleozoic Balcarce Formation, Argentina. Barform–channel type 1 comprises single packages of channel-bar units with inclined beds dipping to the N (paleolandward) and S (paleoseaward), composed predominantly of decimeter-scale planar cross-bedded sandstones. These deposits represent laterally migrating tidal bars. Barform–channel type 2, on the other hand, are multiple-stacked packages of sigmoidal inclined strata dipping to the NE (paleolandward) and SW (paleoseaward), consisting of conglomeratic sandstones with meter-scale trough cross-bedding. These strata depict tidal sand ridges exhibiting oblique accretion dynamics. Tidal barform–channel types 1 and 2 are not interspersed laterally and vertically, but they distinguish the lower and upper intervals of the Balcarce Formation. The lower interval shows full tidal control in barform–channel type 1. W-oriented flow of peak tidal currents completely reworked river-derived sediments and built the bar–trough morphology on the subaqueous platform of a tide-dominated delta. In the upper interval, tide- and river-generated facies alternate in barform–channel type 2. In this case, the higher fluvial input prevented complete tidal reworking. Thus, mouth-bar deposits were partially reworked into tidal ridges, which were organized in sandbanks extending across a tide-influenced delta-front setting. A detailed comparison of tidal barform–channel types 1 and 2, together with a comprehensive re-evaluation of previously published basin-scale data, suggests that the coastal sector where Balcarce tidal barforms accumulated likely formed a strait connected to the open ocean to the east-southeast of the study area.

The Balcarce Formation shows the evolution of tidal barforms from tide-dominated to tide-influenced delta-front settings. While the distinction between tidal barforms has typically relied on their size attributes, the Balcarce barforms offer novel sedimentological and architectural criteria for distinguishing them in tide-dominated and tide-influenced successions. Moreover, the Balcarce barforms highlight tide-generated sand-rich features, thereby expanding the facies spectrum between mud- and sand-rich tide-modulated deltaic successions for comparisons with other deltaic examples, like those developed in broad sandy shelves or straits.
Does Human Resources for Health competence and skill mix status affect compliance with healthcare quality standards among Primary Healthcare Facilities? Evidence from dispensaries in Tanzania
Authors: Mwaisengela Syabo M. , Materu Patricia A. , German Chrisogone J. , Mmbaga Issa I. , Temba Danny J. , Kiwesa Raymond R. , Mapunda Martin S. , Kinyenje Erick S. , Sirili Nathanael , Luoga Pankras W. , Ernest Simon P. , Burra Joseph M. , Hokororo Joseph C. , Kacholi Godfrey , Kapologwe Ntuli A. , Anasel Mackfallen G. , Kumalija Claud J. , Habtu Michael , Saguti Grace E , Masuma Janeth S. , Yahya Talhiya A. , Eliakimu Eliudi S.
Published: December 2024

Background: Globally, it is known that, there is a close correlation between the concentration of qualified Human Resources for Health (HRH) and key health outcomes. Inadequate numbers with poor skill mix compromise health system functioning that negatively affect delivery of quality healthcare services.
Objective: This study aims to ascertain, effect of HRH skills and competence mix to compliance with healthcare quality standards (HCQS) among dispensaries in Tanzania.
Methods: This is a quantitative secondary data analysis using the Star Rating Assessment (SRA) data collected in the fiscal year 2017/2018. Compliance with HCQS was measured by considering service delivery areas of SRA Tool, HRH availability that estimated skill mix status were deduced by considering its recommended number as provided by staffing levels guidelines. Proportions of skill mix status and compliance with HCQS were compared by chi square test while Prevalence Ratios were estimated by Multivariate Poisson Regression with 95% Confidence Interval.
Results: This study involved 4239 dispensaries majority of which were rural located (81.2%) and public owned (83.6%). On average, 36.8% of dispensaries had skill mix while 63.2% had no skill mix. Dispensaries with skill mix were significantly more likely to comply with HCQS compared to dispensaries with no skill mix (p<0.001).
Conclusions: This study demonstrates the critical role that HRH skill mix has in ensuring adherence to HCQS. The observed challenge of HRH availability and hence skill mix should be worked on. Deliberate measures including increasing recruitment budgets and equitable distribution of available HRH should be taken to address the challenge.
The association between PM2.5 and frailty: Evidence from 122 cities in China and 7 countries in Europe
Authors: Wen Yanchao , Zhu Guiming , Cao Kexin , Liang Jie , Lu Xiangfeng , Wang Tong
Published: December 2024

Background: The accelerated aging process worldwide is placing a heavy burden on countries. PM2.5 particulate matter exposure is a significant factor affecting human health and is crucial in the aging process.
Methods: We utilized data from China Health and Retirement Longitudinal Study (CHARLS) and the Survey of Health, Aging, and Retirement in Europe (SHARE) to study the relationship between PM2.5 exposure and the frailty index. Acquire PM2.5 exposure data for China and Europe, match them according to geographic location within the database. Our study used frailty index to evaluate frailty, which comprises 29 items. We examined the association between PM2.5 and frailty index using fixed-effects regression models and Mendelian randomization (MR) analysis.
Results: We first examined the association between PM2.5 and frailty index using fixed-effects regression models, revealing a notable positive link across populations in China (coefficient = 0.0003, P = 0.0380) and Europe (Coefficient = 0.0019, P < 0.0001). This suggests that PM2.5 exposure is a significant risk factor for frailty, leading to accelerated frailty. Moreover, our MR analysis uncovered a possible causal association (OR = 1.2933，95%CI: 1.2045-1.3820, P < 0.0001) between PM2.5 exposure and the frailty index.
Conclusions: Our findings indicate that long-term exposure to PM2.5 in the environment is a risk factor for physical frailty and may have a potential causal relationship. Given the rapid global aging trend, it is crucial to focus on how air pollution affects frailty and to combat its negative consequences.
Maternal Supplementation of Lonicera japonica and Scutellaria Baicalensis Mixed Extracts Improve Reproduction Performance and Metabolic Health through Modulating Gut Microbiota during Pregnancy
Authors: Wang Mu , Yin Shengnan , Cheng Jinghua , Guo Yuqiang , Lv Huiyuan , Jiang Siwen , Peng Jian , Zhou Yuanfei
Published: December 2024

Background
This study aimed to evaluate the impacts of dietary supplementation with a combination of plant extracts on performance, metabolic health, and gut microbiota of sows. One thousand and three crossbred pregnancy sows (DanBred Landrace × DanBred Yorkshire, mean parity 4.44 ± 1.84) were assigned to one of the two dietary treatments: a control group (CON, basal diet) and a Lonicera japonica and Scutellaria baicalensis mixed extracts group (LSE, basal diet supplemented with 0.5 g/kg of mixed extracts). The litter performance at farrowing and weaning were measured. The plasmas samples and feces from sows were obtained on d 30 (G30 d) and 109 (G109 d) of gestation, and d 3 of lactation (L3 d).
Results
Supplementation of LSE increased (P < 0.05) the numbers of total born, litter weight and average pig weight on day 21 of lactation, average daily feed intake, and survival rate of piglets during lactation. Compared to CON, LSE group reduced (P < 0.05) interleukin-6 (IL-6) concentration in the feces and plasmas on G109 d, while increased (P < 0.05) interleukin-10 (IL-10) concentration on G109 d and on L3 d. Supplementation of LSE had lower (P < 0.05) plasmas reactive oxygen species (ROS) levels on G30 d and on L3 d, and had a reducing tendency (P = 0.07) for thiobarbituric acid reactive substances (TBARS) concentrations and a trend toward increased (P = 0.08) value of homeostatic model assessment of insulin sensitivity (HOMA-IS) of sow plasmas on G109 d. In addition, supplementation of LSE increased (P < 0.05) the abundance of Firmicutes and Proteobacteria and decreased (P < 0.05) the abundance of Bacteroidetes and Spirochaetes on G109 d. The abundance of Christensenellaceae_R_7_group, UCG_002, Clostridium_sensu_stricto_1, Escherichia_Shigella, un_f__Christensenellaceae, Bacteroides, and Terrisporobacter were significantly increased (P < 0.05) in the LSE diet group. The abundance of Christensenellaceae_R_7_group, UCG_002 and un_f__Ruminococcaceae were positively correlated with plasma IL-10 (P < 0.01), and negatively correlated with plasma IL-6 and TBARS levels.
Conclusions
Dietary supplementation with LSE diet during gestation improved reproduction performance and health of sows, which might be associated with changes of gut microbiota.