bluuebunny/crossref_metadata_2025 huggingface dataset:
Kidney tea [Orthosiphon aristatus (Blume) Miq.] improves diabetic nephropathy via regulating gut microbiota and ferroptosis
Authors: Zhou Zheng , Niu Hongjuan , Bian Meng , Zhu Chunsheng
Published: June 2024

Introduction

Diabetic nephropathy (DN) is the leading cause of end-stage renal disease. Due to its complex pathogenesis, new therapeutic agents are urgently needed. Orthosiphon aristatus (Blume) Miq., commonly known as kidney tea, is widely used in DN treatment in China. However, the mechanisms have not been fully elucidated.

Methods

We used db/db mice as the DN model and evaluated the efficacy of kidney tea in DN treatment by measuring fasting blood glucose (FBG), serum inflammatory cytokines, renal injury indicators and histopathological changes. Furthermore, 16S rDNA gene sequencing, untargeted serum metabolomics, electron microscope, ELISA, qRT-PCR, and Western blotting were performed to explore the mechanisms by which kidney tea exerted therapeutic effects.

Results

Twelve polyphenols were identified from kidney tea, and its extract ameliorated FBG, inflammation and renal injury in DN mice. Moreover, kidney tea reshaped the gut microbiota, reduced the abundance of Muribaculaceae, Lachnoclostridium, Prevotellaceae_UCG-001, Corynebacterium and Akkermansia, and enriched the abundance of Alloprevotella, Blautia and Lachnospiraceae_NK4A136_group. Kidney tea altered the levels of serum metabolites in pathways such as ferroptosis, arginine biosynthesis and mTOR signaling pathway. Importantly, kidney tea improved mitochondrial damage, increased SOD activity, and decreased the levels of MDA and 4-HNE in the renal tissues of DN mice. Meanwhile, this functional tea upregulated GPX4 and FTH1 expression and downregulated ACSL4 and NCOA4 expression, indicating that it could inhibit ferroptosis in the kidneys.

Conclusion

Our findings imply that kidney tea can attenuate DN development by modulating gut microbiota and ferroptosis, which presents a novel scientific rationale for the clinical application of kidney tea.
Understanding patient demand for and use of antibiotics for upper respiratory tract infection: A qualitative application of the Necessity-Concerns Framework in Saudi Arabia
Authors: Almeshal Nouf , Foot Holly , Clarke Amy Louise , Chan Amy Hai Yan , Horne Rob
Published: June 2024

Background

Reducing antimicrobial resistance (AMR) is a priority for public health. Inappropriate patient demand is an important driver of unnecessary antibiotic use. To develop an effective intervention to reduce inappropriate demand for antibiotics in upper respiratory tract infections (URTIs), it is important to identify patient perceptions that influence demand for, and appropriate use of antibiotics.

Aim

To identify and describe the beliefs about antibiotics necessity and concerns that patients with URTIs have, in Riyadh, Saudi Arabia.

Method

An exploratory qualitative approach was used. One-to-one, face-to-face or telephone semi-structured interviews were conducted with participants recruited using purposive sampling (based on age and gender) from primary healthcare centre in Riyadh, Saudi Arabia were conducted. Only adult patients who currently experience URTIs symptoms and agreed to participate were recruited. Recruitment for interviews continued until data saturation point was reached. The interview guide explored patients’ necessity beliefs and concerns about antibiotics, AMR perceptions, and expectations from URTIs consultation. Interview transcripts were coded using QSR NVivo 12 using framework analysis informed by the Necessity-Concerns Framework to identify key motivations driving antibiotic requests and consultations.

Results

the study interviewed 32 participants (44% were male, average age was 36.84). Results identified that the patients often relate their personal need for antibiotics when encountering an URTIs symptoms to the type, severity and duration of symptoms. Patients also linked antibiotics with quicker recovery, generally expressing few concerns about antibiotics mainly because of its short duration of use. However, some conveyed their concern about frequent administration of antibiotics and effect on the body’s immune system function, which may make them more prone to infections in the future. Participants varied widely in their awareness of AMR; this was associated with many misconceptions, such as confusing AMR with antibiotics efficacy and tolerance. Interestingly, the interplay between necessity beliefs and concerns was observed to influence the decision to start and stop antibiotic, potentially impacting inappropriate antibiotic demand and unnecessary use.

Conclusion

This study highlighted important beliefs and misconceptions about antibiotics and AMR in Saudi population which can be targeted in future interventions to reduce inappropriate demand for antibiotics and optimise appropriate usage.
Ferroptosis resistance in cancer cells: nanoparticles for combination therapy as a solution
Authors: Adzavon Kodzo Prosper , Zhao Weijian , He Xuesong , Sheng Wang
Published: June 2024

Ferroptosis is a form of regulated cell death (RCD) characterized by iron-dependent lipid peroxidation. Ferroptosis is currently proposed as one of the most promising means of combating tumor resistance. Nevertheless, the problem of ferroptosis resistance in certain cancer cells has been identified. This review first, investigates the mechanisms of ferroptosis induction in cancer cells. Next, the problem of cancer cell resistance to ferroptosis, as well as the underlying mechanisms is discussed. Recently discovered ferroptosis-suppressing biomarkers have been described. The various types of nanoparticles that can induce ferroptosis are also discussed. Given the ability of nanoparticles to combine multiple agents, this review proposes nanoparticle-based ferroptosis cell death as a viable method of circumventing this resistance. This review suggests combining ferroptosis with other forms of cell death, such as apoptosis, cuproptosis and autophagy. It also suggests combining ferroptosis with immunotherapy.
Harnessing the power of natural alkaloids: the emergent role in epilepsy therapy
Authors: Li Siyu , Lin Xinyu , Duan Lijuan
Published: June 2024

Graphical Abstract

Therapeutic mechanisms of natural alkaloids in epilepsy treatment.
Efficacy of virtual reality exercise in knee osteoarthritis rehabilitation: a systematic review and meta-analysis
Authors: Wei Wei , Tang Haiting , Luo Yu , Yan Shichang , Ji Qipei , Liu Zhixiang , Li Huaqiang , Wu Fei , Yang Shenqiao , Yang Xin
Published: June 2024

Background

This systematic review and meta-analysis aims to investigate the effects of virtual reality (VR) exercise compared to traditional rehabilitation on pain, function, and muscle strength in patients with knee osteoarthritis (KOA). Additionally, the study explores the mechanisms by which VR exercise contributes to the rehabilitation of KOA patients.

Methods

We systematically searched PubMed, the Cochrane Library, Embase, Web of Science, Scopus, and PEDro according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Our search spanned from the library construction to 24 May 2024, focusing on randomized controlled trials Primary outcomes included pain, Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), and muscle strength. Meta-analysis was conducted using RevMan (version 5.4) and Stata (version 14.0). The bias risk of included studies was assessed using the Cochrane RoB 2.0 tool, while the quality of evidence was evaluated using the Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) approach.

Results

This meta-analysis and systematic review included nine studies involving 456 KOA patients. The results indicated that VR exercise significantly improved pain scores (SMD, −1.53; 95% CI: −2.50 to −0.55; p = 0.002), WOMAC total score (MD, −14.79; 95% CI: −28.26 to −1.33; p = 0.03), WOMAC pain score (MD, −0.93; 95% CI: −1.52 to −0.34; p = 0.002), knee extensor strength (SMD, 0.51; 95% CI: 0.14 to 0.87; p = 0.006), and knee flexor strength (SMD, 0.65; 95% CI: 0.28 to 1.01; p = 0.0005), but not significantly for WOMAC stiffness (MD, −0.01; 95% CI: −1.21 to 1.19; p = 0.99) and physical function (MD, −0.35; 95% CI: −0.79 to −0.09; p = 0.12).

Conclusion

VR exercise significantly alleviates pain, enhances muscle strength and WOMAC total score in KOA patients, but improvements in joint stiffness and physical function are not significant. However, the current number of studies is limited, necessitating further research to expand on the present findings.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42024540061, identifier CRD42024540061
Perinatal influences on academic achievement and the developing brain: a scoping systematic review
Authors: Schneider Deborah , Bouhali Florence , Richter Caroline G. , Costache Radu , Costache Catalina , Kirchhoffer Kaitlyn , Sheth Vatsa , MacDonald Ibo , Hoeft Fumiko
Published: June 2024

Introduction and methods

In this PRISMA-compliant systematic review, we identify and synthesize the findings of research in which neuroimaging and assessments of achievement have been used to examine the relationships among aspects of developmental programming, neurodevelopment, and achievement in reading and mathematics.

Results

Forty-seven studies met inclusion criteria. The majority examined the impact of prematurity (n = 32) and prenatal alcohol exposure (n = 13). Several prematurity studies reported a positive correlation between white-matter integrity of callosal fibers and executive functioning and/or achievement, and white matter properties were consistently associated with cognitive and academic performance in preterm and full-term children. Volumetric studies reported positive associations between academic and cognitive abilities and white and gray matter volume in regions such as the insula, putamen, and prefrontal lobes. Functional MRI studies demonstrated increased right-hemispheric language processing among preterm children. Altered activation of the frontoparietal network related to numerical abilities was also reported. Prenatal alcohol exposure studies reported alterations in white matter microstructure linked to deficits in cognitive functioning and academic achievement, including mathematics, reading, and vocabulary skills. Volumetric studies reported reductions in cerebral, cerebellar, and subcortical gray matter volumes associated with decreased scores on measures of executive functioning, attention, working memory, and academic performance. Functional MRI studies demonstrated broad, diffuse activation, reduced activation in canonical regions, and increased activation in non-canonical regions during numeric tasks.

Discussion

A preponderance of studies linked prematurity and prenatal alcohol exposure to altered neurodevelopmental processes and suboptimal academic achievement. Limitations and recommendations for future research are discussed.

Systematic review registration

Identifier: DOI 10.17605/OSF.IO/ZAN67.
A bibliometric analysis of speech and language impairments in Parkinson’s disease based on Web of Science
Authors: Pan Xueyao , Liang Bingqian , Cao Ting
Published: June 2024

Many individuals with Parkinson’s disease suffer from speech and language impairments that significantly impact their quality of life. Despite several studies on these disorders, there is a lack of relevant bibliometric analyses. This paper conducted a bibliometric analysis of 3,610 papers on speech and language impairments in Parkinson’s disease patients from January 1961 to November 2023, based on the Web of Science Core Collection database. Using Citespace software, the analysis focused on annual publication volume, cooperation among countries and institutions, author collaborations, journals, co-citation references, and keywords, aiming to explore the current research status, hotspots, and frontiers in this field. The number of annual publications related to speech and language impairment in Parkinson’s disease have been increasing over the years. The USA leads in the number of publications. Research hotspots include the mechanism underlying speech and language impairments, clinical symptoms, automated diagnosis and classification of patients with PD using linguistic makers, and rehabilitation interventions.
Taking the edge off: a feasibility randomized controlled trial of an online mindfulness-based intervention to reduce suspiciousness/paranoia in high positive schizotypy
Authors: McDonald Heather , Valmaggia Lucia , Antonova Elena , Chadwick Paul
Published: June 2024

Positive schizotypy can uniquely predict the development of psychosis with suspiciousness/paranoia having emerged as a key risk factor, pointing to significant worth in reducing this aspect in individuals with high positive schizotypy. Reduced paranoia in the general population following brief online mindfulness training has been previously reported. This study investigated the feasibility of a 40-day online mindfulness-based intervention (MBI) (n = 12) in the individuals with high positive schizotypy characterized by high suspiciousness/paranoia and to estimate its effect on paranoia as compared with an active control condition using reflective journaling (n = 12). The outcome measures were self-reported trait and VR-induced state paranoia, completed at baseline, after 10 days and post-intervention. The feasibility criteria included retention, adherence, engagement, and acceptability. There was 100% retention, excellent adherence to content and engagement, with an average MBI session completion rate of 91%. Acceptability, indexed by a self-rated motivation to continue practice post-intervention, was also high. No MBI effect on trait paranoia was observed; however, the MBI group showed a reduction in the VR-induced state paranoia with a medium-to-large effect (d = 0.63). The findings support conducting larger-scale randomized controlled trials to evaluate the effects of online MBIs on reducing suspiciousness/paranoia to mitigate psychosis risk in individuals with high positive schizotypy.

Clinical Trial Registration:https://www.isrctn.com/, identifier ISRCTN78697391.
Emission characteristics and health effects of PM2.5 from vehicles in typical areas
Authors: Song Xiaowei , Hao Yongpei
Published: June 2024

Introduction

Vehicle emissions have become an important source of urban air pollution, and the assessment of air pollution emission characteristics and health effects caused by specific pollution sources can provide scientific basis for air quality management.

Methods

In this paper, vehicle PM2.5 pollution in typical urban agglomerations of China (the Beijing-Tianjin-Hebei urban agglomeration (BTHUA), the triangle of the Central China urban agglomeration (TCCUA) and the Chengdu-Chongqing urban agglomeration (CCUA)) were used as research samples to evaluate the emission characteristics, health effects and economic losses of vehicle PM2.5 pollution based on the emission inventory, air quality model and exposure-response function from 2010 to 2020.

Results

The results indicated that PM2.5 emissions from vehicles in the three urban agglomerations during 2010-2020 first showed an upward yearly trend and then showed a slow decrease in recent years. Heavy-duty trucks and buses are the main contribution vehicles of PM2.5, and the contribution rates of light-duty vehicles to PM2.5 is increasing year by year. The contribution rate of PM2.5 in Beijing decreased significantly. In addition to capital cities and municipalities directly under the central Government, the emission of pollutants in other cities cannot be ignored. The evaluation results of the impact of PM2.5 pollution from vehicles on population health show that: the number of each health endpoint caused by PM2.5 pollution from vehicles in the BTHUA and CCUA showed an overall upward trend, while the TCCUA showed a downward trend in recent years. Among them, PM2.5 pollution from vehicles in the three major urban agglomerations cause about 78,200 (95% CI: 20,500-138,800) premature deaths, 122,800 (95% CI: 25,600-220,500) inpatients, and 628,400 (95% CI: 307,400-930,400) outpatients and 1,332,400 (95% CI: 482,700-2,075,600) illness in 2020. The total health economic losses caused by PM2.5 pollution from vehicles in the three major urban agglomerations in 2010, 2015 and 2020 were 68.25 billion yuan (95% CI: 21.65-109.16), 206.33 billion yuan (95% CI: 66.20-326.20) and 300.73 billion yuan (95% CI: 96.79-473.16), accounting for 0.67% (95% CI: 0.21-1.07%), 1.19% (95% CI: 0.38%-1.88%) and 1.21% (95% CI: 0.39%-1.90%) of the total GDP of these cities.

Discussion

Due to the differences in vehicle population, PM2.5 concentration, population number and economic value of health terminal units, there are differences in health effects and economic losses among different cities in different regions. Among them, the problems of health risks and economic losses were relatively prominent in Beijing, Chengdu, Chongqing, Tianjin and Wuhan.
Evaluating China's primary healthcare services' efficiency and spatial correlation: a three-stage DEA-Malmquist model
Authors: Huang Rui , Li Wan , Shi Baoguo , Su Hao , Hao Jing , Zhao Chuanjun , Chai Juhong
Published: June 2024

Introduction

Enhancing the efficiency of primary healthcare services is essential for a populous and developing nation like China. This study offers a systematic analysis of the efficiency and spatial distribution of primary healthcare services in China. It elucidates the fundamental landscape and regional variances in efficiency, thereby furnishing a scientific foundation for enhancing service efficiency and fostering coordinated regional development.

Methods

Employs a three-stage DEA-Malmquist model to assess the efficiency of primary healthcare services across 31 provincial units in mainland China from 2012 to 2020. Additionally, it examines the spatial correlation of efficiency distribution using the Moran Index.

Results

The efficiency of primary healthcare services in China is generally suboptimal with a noticeable declining trend, highlighting significant potential for improvement in both pure technical efficiency and scale efficiency. There is a pronounced efficiency gap among provinces, yet a positive spatial correlation is evident. Regionally, efficiency ranks in the order of East > Central > West. Factors such as GDP per capita and population density positively influence efficiency enhancements, while urbanization levels and government health expenditures appear to have a detrimental impact.

Discussion

The application of the three-stage DEA-Malmquist model and the Moran Index not only expands the methodological framework for researching primary healthcare service efficiency but also provides scientifically valuable insights for enhancing the efficiency of primary healthcare services in China and other developing nations.
Coopetition, exploration and exploitation capabilities, and growth performance in digital healthcare ventures
Authors: Liu Xiangmin , Bai Li , Leng Xiaoning , Yao Yexiang , Yang Yue , Li Debao , Yin Haobo
Published: June 2024

Introduction

Studies focusing on coopetition and dynamic capabilities have expanded significantly over the past several decades. Coopetition strategy and dynamic capabilities are increasingly recognised as sources of sustained competitive advantage. The purpose of this paper is to provide a better understanding of the factors driving growth performance in digital healthcare ventures by examining the role of coopetition, exploration and exploitation capabilities, and environmental uncertainty. While numerous studies have examined the competitive advantage of coopetition, its specific contribution to the growth of ventures in the digital realm remains less explored. Clarifying the strategic role of coopetition in driving growth performance is critical for delineating the intricate relationship between coopetition and growth performance, particularly in the context of digital healthcare ventures. To fill in this research gap, this study uses coopetition theory and dynamic capabilities theory to look at how exploration and exploitation capabilities, as well as environmental uncertainty, affect the relationship between coopetition and growth performance in digital healthcare ventures.

Methods

We collected a total of 338 questionnaires from Chinese digital healthcare ventures between March 2023 and August 2023. We conducted data analysis using SPSS 26.0 and its macro-program PROCESS.

Results

Our results confirm that coopetition has a positive effect on growth performance in digital healthcare ventures. Furthermore, exploration and exploitation capabilities fully mediate the relationship between coopetition and growth performance. Moreover, environmental uncertainty significantly and distinctively moderates the impact of exploration and exploitation capabilities on growth performance.

Discussion

This study contributes to the existing literature by providing deeper insight into the relationship between coopetition and growth performance in digital healthcare ventures. It also offers important practical implications for public health improvement and socio-economic development.
Oral health education strategies for patients living with cardiovascular disease within hospital settings: a scoping review
Authors: Church L. A. , Robins L. , Xu F. , Qin L. , Tran A. , Wallace J. P. , King S.
Published: June 2024

Objective

To identify and describe the impact of current oral health education programmes provided to patients in cardiology hospital wards and outpatient clinics.

Methods

This review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analysis for Scoping Reviews statement. Searches were conducted using electronic databases: Cochrane, Medline, and Scopus, as well as grey literature searching.

Results

Three eligible studies were identified. All included studies reported generalised poor oral health in their participants at baseline, with significant improvement at follow-up. They all reported significant reductions in plaque deposits and gingival bleeding. One study reported significantly less bacteria on participant tongues, as well as fewer days with post-operative atrial fibrillation in the intervention group. Furthermore, in this study, one patient in the intervention group developed pneumonia, whilst four patients in the control group did.

Conclusion

Oral health education for patients with cardiovascular disease is limited and many have poor oral health. Educational programmes to improve oral health behaviours in patients with cardiovascular disease can improve both oral and general health outcomes.

Implications for public health

Oral disease is a modifiable risk factor for cardiovascular disease. Integrating oral health education into cardiology hospital settings is a simple strategy to improve access to oral health information and improve both oral and cardiovascular outcomes.
Occupational health disorders among physical education teachers compared to classroom and subject specialist teachers
Authors: Markelj Neja , Kovač Marjeta , Leskošek Bojan , Jurak Gregor
Published: June 2024

During the course of their work, teachers may be subjected to conditions that cause different health problems. This study examines occupational health disorders in a representative sample of 858 teachers (528 female; age 44.0 ± 9.67 years) divided into three groups of teachers with specific occupational requirements: specialist physical education teachers (specialist PETs), classroom teachers, and specialist teachers. The number of health disorders in the last 12 months was recorded using the Chronic Health Disorders Questionnaire. The differences between the different types of teachers, controlled for sex and age, were analyzed using binary logistic regression. The results showed that 89% of teachers experienced colds as the most frequently reported health problem, followed by 58% for lower back problems, 57% for headaches, 51% for hoarseness, and 43% for neck problems. A binary logistic regression showed that specialist PETs were the group with the highest health risk. They were about twice as likely to have musculoskeletal or hearing disorders than the other two groups of teachers. They were also significantly more likely to suffer from hoarseness. Understanding these different health challenges is critical to developing targeted interventions and robust support systems. These interventions should include initiatives aimed at raising awareness of health risk factors, implementing injury interventions and vocal cord hygiene programs, making ergonomic adjustments, and promoting awareness of self-care (both mental and physical). Given that the teaching profession is currently struggling with an aging workforce and a shortage of teachers, addressing these challenges is critical to the continued well-being of the teaching professionals.
Racial disparities in colorectal cancer outcomes and access to care: a multi-cohort analysis
Authors: Riviere Paul , Morgan Kylie M. , Deshler Leah N. , Demb Joshua , Mehtsun Winta T. , Martinez Maria Elena , Gupta Samir , Banegas Matthew , Murphy James D. , Rose Brent S.
Published: June 2024

Introduction

Non-Hispanic Black (NHB) Americans have a higher incidence of colorectal cancer (CRC) and worse survival than non-Hispanic white (NHW) Americans, but the relative contributions of biological versus access to care remain poorly characterized. This study used two nationwide cohorts in different healthcare contexts to study health system effects on this disparity.

Methods

We used data from the Surveillance, Epidemiology, and End Results (SEER) registry as well as the United States Veterans Health Administration (VA) to identify adults diagnosed with colorectal cancer between 2010 and 2020 who identified as non-Hispanic Black (NHB) or non-Hispanic white (NHW). Stratified survival analyses were performed using a primary endpoint of overall survival, and sensitivity analyses were performed using cancer-specific survival.

Results

We identified 263,893 CRC patients in the SEER registry (36,662 (14%) NHB; 226,271 (86%) NHW) and 24,375 VA patients (4,860 (20%) NHB; 19,515 (80%) NHW). In the SEER registry, NHB patients had worse OS than NHW patients: median OS of 57 months (95% confidence interval (CI) 55–58) versus 72 months (95% CI 71–73) (hazard ratio (HR) 1.14, 95% CI 1.12–1.15, p = 0.001). In contrast, VA NHB median OS was 65 months (95% CI 62–69) versus NHW 69 months (95% CI 97–71) (HR 1.02, 95% CI 0.98–1.07, p = 0.375). There was significant interaction in the SEER registry between race and Medicare age eligibility (p < 0.001); NHB race had more effect in patients <65 years old (HR 1.44, 95% CI 1.39–1.49, p < 0.001) than in those ≥65 (HR 1.13, 95% CI 1.11–1.15, p < 0.001). In the VA, age stratification was not significant (p = 0.21).

Discussion

Racial disparities in CRC survival in the general US population are significantly attenuated in Medicare-aged patients. This pattern is not present in the VA, suggesting that access to care may be an important component of racial disparities in this disease.
Intersubjectivity as an analytical concept to study human-animal interaction in historical context: street dogs in Late Ottoman period
Authors: Taşdizen Burak , Yetiş Erman Örsan , Bakırlıoğlu Yekta
Published: June 2024

Human knowledge pertaining to human-animal interaction is constructed by the human author, albeit the presence of animal subjects. Such a human lens is pronounced when studying human-animal interactions across history, whose nonhuman animal subjects are not only absent, and therefore eliminating the possibility of conducting empirical studies in situ, but also their experiences are filtered by the interpretative lens of human authors of extant historical accounts as well as contemporary human analysts who interpret these accounts. This article draws upon such epistemological limitations of understanding nonhuman animal presence in historical accounts and offers human-animal intersubjectivity as an analytical concept, involving generative iterability and indistinctive boundaries that emphasise intersubjective openness and relationality, to trace and disclose the continuity of human-animal co-existence. The article’s historical scope is the Late Ottoman period characterised by a sense of temporal and spatial disorientation and reorientation for humans as well as street dogs during its modernisation processes.
Immune checkpoint regulation is critically involved in canine cutaneous histiocytoma regression
Authors: Diehl Benjamin , Hansmann Florian
Published: June 2024

Introduction

Canine cutaneous histiocytoma (CCH) is a benign tumor frequently occurring in young dogs which is derived from Langerhans cells (LC). Distinguishing features of this tumor are its spontaneous regression following a rapid tumor growth. Impaired control of immune checkpoints during tumor development and progression is a widespread phenomenon which may result in an absent or ineffective immune response. The interaction between the inflammatory response and the expression of immune checkpoint molecules is only partially described in this tumor type. The aim of this study was to identify immune checkpoint molecules and molecules from the interferon-mediated immune response that are involved in the regression of CCH.

Methods

Forty-eight CCH derived from dogs ≤ 4 years of age were assigned to one of four groups according to the severity and distribution of lymphocyte infiltration. Using immunohistochemistry and whole-slide image scans of consecutive sections the expression of programmed death protein ligand 1 (PD-L1), CD80, CD86, Survivin, forkhead box protein 3, Ki-67, cleaved caspase-3, CD3, and mx1 were investigated. RNA in-situ hybridization was performed for transcripts of mx1 and interferon-γ.

Results

Neoplastic cells showed an expression of PD-L1, CD80, CD86, and Survivin. The density of CD80 expressing cells was negatively correlated with regression while the density of cleaved caspase-3 positive cells increased with regression. Mx1 transcripts and protein were predominantly localized in neoplastic cells while interferon-γ transcripts were most frequently detected in T-cells.

Conclusion

The expression of the immune checkpoint molecules CD86 and PD-L1 and particularly the reduced expression of CD80 in groups 3 and 4 indicate an influence of the investigated immune checkpoints on tumor regression. In parallel an activation of the apoptotic cascade during regression is suggested. Finally, the detection of mx1 within the neoplasm pinpoints to a yet undisclosed role of anti-cellular signaling in tumor immunity.
Evaluation of sterile glove usage on digital tactile sensitivity using the Grating Orientation Task
Authors: Riegel Thomas O. , Zellner Eric M. , Hedlund Cheryl S. , Kraus Karl H.
Published: June 2024

Introduction

Surgical glove use may be associated with a decrease in tactile sensitivity, with thicker gloves or double-gloving techniques further altering sensation. This study evaluates digital tactile sensitivity by use of a Grating Orientation Task (GOT) with multiple sterile gloving techniques (no gloves, single standard gloving, double standard gloving, orthopedic gloves, and micro-thickness gloves).

Methods

Each participant performed the GOT at increasing grating widths until correctly noting orientation in ≥8 of 10 trials with multiple glove types or double-gloving technique. Glove order was randomly assigned and participants were blinded to the orientation and dome size.

Results

All gloves except micro-thickness gloves showed increased threshold sensitivity values (i.e. worse fingertip sensitivity) when compared to control (micro:control, p = 0.105, others:control, p < 0.05). Single-layer gloves showed no significant difference in sensitivity when compared to orthopedic (p = 0.06) or double-layer latex gloves (p = 0.26).

Discussion

Standard latex gloves decreased fingertip sensitivity when evaluated with the GOT. Double-layer and orthopedic latex gloves do not decrease sensitivity when compared with single-layer gloving. Micro-thickness gloves may provide similar tactile sensitivity to no surgical glove.
Case report: Management and long-term ophthalmic sequelae of monogenean ocular infestation in cownose rays (Rhinoptera bonasus)
Authors: Beatty April , Gemensky-Metzler Anne , Newbold Georgina , Aplasca Andrea C. , Seeley Kathryn E.
Published: June 2024

Monogenean ectoparasitic flatworm infestations, particularly in closely confined populations, can result in severe epizootic disease that is often devastating and occasionally fatal. This case series describes a population of cownose rays (Rhinoptera bonasus) (n = 52) housed in an aquarium touch-tank exhibit that presented with severe ocular disease due to infestation with monogeneans, presumably Benedeniella posterocolpa of the Capsilidae family. The most severely affected individuals (n = 9), including several cases with bilaterally ruptured corneas, underwent serial examinations prior to and following treatment with praziquantel, systemic antibiotics, and corticosteroids. The entire population underwent frequent therapeutic water changes with a scheduled decrease in salinity, increase in temperature, and a series of seven praziquantel tank treatments. At the last follow up examination (3.75 years), the most common ocular findings were corneal fibrosis (18/18 eyes; 100%), cataract formation (13/18 eyes; 72.2%), synechia (8/18 eyes; 44.4%), and dyscoria (5/18 eyes; 27.8%). Despite severe corneal disease, including corneal rupture, all examined eyes (18/18; 100%) showed remarkable corneal remodeling and a largely clear visual axis. There are very few reports describing corneal disease in aquarium housed elasmobranchs, and no reports describe ophthalmic implications of monogenean infestation in these animals. This further underscores the importance of this case series in demonstrating the capacity for healing of elasmobranch eyes and can provide further guidance regarding prognosis and treatment in cases of severe corneal disease.
Bridging the Gap: Exploring the Impact of Human Capital Management on Employee Performance through Work Engagement
Authors: Isah Leontes Njanjobea , Hoole Crystal
Published: June 2024

The aim of this study was to suggest and test a research model that examines whether work engagement mediates the effect of human capital management practices (HCMPs) on employee performance. These relationships were assessed through the Smart PLS 4 (Partial Least Square) software using Partial Least Square-based Structural Equation Modelling (PLS-SEM). Data were elicited from full-time hotel employees and their managers in South Africa. Based on the PLS-SEM results, work engagement was identified as a mediator of the effects of HCMPs on employee performance. Specifically, HCMPs, manifested by training, career opportunities, and rewards, improve work engagement, triggering employee tasks, and adaptive and creative performance. Additionally, work engagement had a positive influence on task performance, adaptive performance, and contextual performance. This research contributes by empirically testing work engagement as a full mediator in the relationship between HCMPs and employee performance, offering insights into the crucial role of HCMPs in fostering employee engagement and performance within the hospitality industry. Implications of the results are discussed, and directions for future research are presented.
Investigation on Accelerated Initiation of Oblique Detonation Wave Induced by Laser-Heating Hot-Spot
Authors: Xin Yirong , Shang Jiahao , Xiang Gaoxiang , Wang Qiu
Published: June 2024

A reliable initiation of oblique detonation is critical in oblique detonation engines, especially for oblique detonation engines under extreme conditions such as a high altitude and low Mach number, which may lead to excessive length of the induction zone and even the phenomenon of extinction. In this paper, surface ignition was applied to the initiation of oblique detonation, and a high-temperature region was set on the wedge to simulate the presence of a hot-spot induced by the laser heating. The two-dimensional multi-component Navier–Stokes equations considering a detailed H2 combustion mechanism are solved, and the oblique detonation wave accelerated by a hot-spot is studied. In this paper, hot-spots in the induction zone on the wedge, are introduced to explore the possibility of hot-spot initiation, providing a potential method for initiation control. Results show that these methods can effectively promote the accelerated initiation of the oblique detonation. Furthermore, the hot-spot temperature, size and position are varied to analyze their effects on the initiation position. Increasing the temperature and size of the hot-spot both can accelerate initiation, but from the perspective of energy consumption, a small hot-spot at a high temperature is preferable for accelerating ODW initiation than a large hot-spot at a low temperature. The initiated position of the oblique detonation is sensitive to the position of the hot-spots; if a 2000 K hotspot is at the beginning of the wedge, then the ODW’s initiation distance will be reduced to about 30% of that without hotspot acceleration.
Coaxial Helicopter Attitude Control System Design by Advanced Model Predictive Control under Disturbance
Authors: Chen Zhi , Lin Xiangyu , Jiang Wanyue
Published: June 2024

This paper proposes an advanced model predictive control (MPC) scheme for the attitude tracking of coaxial drones under wind disturbances. Unlike most existing MPC setups, this scheme embeds steady-input, steady-output, and steady-state conditions into the optimization problem as decision variables. Consequently, the coaxial drone’s attitude can slide along the state manifold composed of a series of steady states. This allows it to move toward the optimal reachable equilibrium. To address disturbances that are difficult to accurately measure, an extended state observer is employed to estimate the disturbances in the prediction model. This design ensures that the algorithm maintains recursive stability even in the presence of disturbances. Finally, numerical simulations and flight tests are provided to confirm the effectiveness of the proposed method through comparison with other control algorithms.
Analytic Solutions for Volume, Mass, Center of Gravity, and Inertia of Wing Segments and Rotors of Constant Density
Authors: Moulton Benjamin C. , Hunsaker Douglas F.
Published: June 2024

In the preliminary design of aircraft lifting surfaces, accurate mass and inertia properties can be difficult to obtain. Typically, such methods as computer-aided design or statistical processes are used to determine these properties. These methods require significant time and effort to implement. The present paper presents an exact analytic method for calculating the volume, mass, center of gravity, and inertia properties of wing segments and rotors of constant density. The influence of taper, spanwise thickness distribution, airfoil geometry, and sweep are included. The utility of the method is presented, and the accuracy is evaluated with various test cases via percent difference with a corresponding computer-aided design model. These case studies demonstrate the present method to be accurate to within about 1% for typical wing geometries and within about 1.3% for typical propeller geometries.
Prohexadione Calcium and Gibberellin Improve Osmoregulation, Antioxidant Response and Ion Homeostasis to Alleviate NaCl Stress in Rice Seedlings
Authors: Liu Meiling , Feng Naijie , Zheng Dianfeng , Zhang Rongjun
Published: June 2024

Prohexadione calcium (EA) and gibberellin (GA) are two different types of plant growth regulators that have different effects on the regulation of plant development. The objective of this study was to evaluate the impacts of EA and GA on rice plant growth, development and morph-physiological traits in two rice varieties: ‘Huang Huazhan’ and ‘Guang Hong 3’. At the three-leaf seedling stage, the plants were treated with 50 mM NaCl 24 h after foliar application of EA (100 mg·L−1) and GA (1 mg·L−1). Data on morphological indexes, osmotic regulators and antioxidant activities were compared with the treatment of EA and GA on the 4th, 7th, 10th and 13th days after NaCl stress. Our data analysis showed that NaCl stress inhibited the leaf area growth of rice seedlings, altered the microstructure and disrupted the antioxidant system, ion uptake and transport balance. The significant increase in malondialdehyde (MDA) content and superoxide anion production rate (O2·¯) indicated that NaCl stress caused a severe oxidative stress response to rice seedlings. Treatment with EA and GA activated the antioxidant system under NaCl stress, significantly elevated superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities and suppressed the increase in MDA content and the O2·¯ production rate. Under NaCl stress, EA and GA treatments improved the osmoregulatory balance, significantly increased soluble protein and proline contents and maintained lower Na+/K+ levels. EA and GA treatments significantly increased the K+ and Ca2+ contents, thereby maintaining ionic balance, which was favorable for maintaining the growth of rice seedlings. In this study, moth plant growth regulators maintained the growth and development of rice seedlings under NaCl stress by inducing an increase in osmoregulation and antioxidant levels, reducing the degree of membrane damage and regulating the selective uptake of ions by rice seedlings. Current findings also clarified that foliar application of EA was more effective than GA in three-leaf seedlings by enhancing the morph-physiological and antioxidant parameters under NaCl stress.
Submergence Stress Reduces the Ability of Rice to Regulate Recovery after Disaster
Authors: Yu Yanmei , Meng Yan , Chen Peng , Cao Kaihua
Published: June 2024

Flood submergence has devastating effects on agricultural production in China, with rice being particularly vulnerable to its impacts. Previous studies on rice submergence stress have primarily focused on immediate yield reduction and short-term growth. In this study, a submergence stress experiment was carried out by using the method of potted rice flooding. The growth recovery characteristics of rice under different submergence stress were analyzed through the continuous observation of rice growth after the disaster. The results showed that submergence stress had a persistent effect on rice growth, which persisted until the recovery period after the disaster. The recovery ability of rice plants decreased with the aggravation of stress, leading to increased damage to the plant. The average yield decreased by 17.07% and 15.56% due to submergence stress during the jointing and booting stage, respectively. The current study pointed out that the growth traits of and, furthermore, the mechanism of physiological changes in rice during the recovery period need to be explored in order to understand the effects of flooding stress on rice.
The Infection of Barley at Different Growth Stages by Bipolaris sorokiniana and Its Effect on Plant Yield and Sowing Value
Authors: Wiewióra Barbara , Żurek Grzegorz
Published: June 2024

Bipolaris sorokiniana, a barley pathogen, can infect via seeds, co-growing plants, or soil, causing yield and seed value reductions. This study aimed to assess B. sorokiniana’s impact at various growth stages on seed yield and quality. Results showed no direct impact on yield (which ranged from 0.49 kg·m−2 for the naked variety Rastik to 0.77 kg·m−2 for the Widawa variety), but revealed significant seed quality differences. Thousand kernel weight (TKW) and germination capacity (GC) varied notably between examined varieties. The largest kernels were observed for the Bryl variety (27.33 g), which also had the best germination (82.8%). The variety Ryton had the smallest kernels (24.04 g), while the poorest germination (56.7%) was observed for naked kernels of Rastik. A seed health analysis found a relation between inoculation stage and the intensity of spontaneous infection by pathogenic fungi, ranging from 86.4% for the control to more than 95% for the kernels harvested from plants inoculated at the flowering stage. Strong correlation emerged between B. sorokiniana grain infection and germination capacity, highlighting the fungus’s role in seed quality decline.
Winter Wheat Aboveground-Biomass Estimation and Its Dynamic Variation during Coal Mining—Assessing by Unmanned Aerial Vehicle-Based Remote Sensing
Authors: Lyu Xiaoxuan , Zhang Hebing , Chen Zhichao , Jiao Yiheng , Du Weibing , Zhang Xufei , Luo Jialiang , Zhang Erwei
Published: June 2024

Underground coal mining in coal-grain overlapped areas leads to land subsidence and deformation above the goaf, damaging cultivated land. Understanding the influencing process of coal mining on cultivated land and crops is important for carrying out timely land reclamation and stabilizing crop yield. Research has been carried out by using crop growth parameters to evaluate the damaging degree of cultivated land when the mining subsidence is stable, but few studies focus on the influence of land damage on crop growth when the subsidence is unstable during coal mining. Therefore, this study tracked the three growth stages of winter wheat by using UAV multispectral imagery to explore the dynamic influence of underground mining on winter wheat aboveground biomass (AGB). Firstly, a winter-wheat-AGB estimation model (R2 = 0.89, RMSE = 2.18 t/ha) was developed by using vegetation indexes (VIs), textures, and terrain data extracted from UAV imagery. Secondly, based on the winter-wheat-AGB estimation model, the winter wheat AGB was successfully estimated and mapped at different growth stages. The AGB of winter wheat in the coal mining-affected area was approximately 5.59 t/ha at the reviving stage, 8.2 t/ha at the jointing stage, and 15.6 t/ha at the flowering stage. Finally, combined with the progress of coal mining, the dynamic changing process of crops during underground mining can be inferred by analyzing the spatiotemporal variation in winter wheat AGB. Results showed that, in the dip direction, winter wheat AGB at the flowering stage was the highest at the compression zone, followed by the inner stretch zone, outer stretch zone, and neutral zone. The distance from the waterlogged area and the existence of cracks were found to be the important moderating variables affecting the crop growth status in the mining subsidence area. In the strike direction, there were significant differences in the wheat AGB-affected area as the mining proceeded. Even areas where AGB had previously significantly increased gradually transitioned to significant decreases with the end of mining. The research explores the dynamic changes in winter wheat AGB and land damage status during coal mining. It provides a rapid and non-destructive land-damage-monitoring method to protect cultivated land in mining areas.