bluuebunny/crossref_metadata_2025 huggingface dataset:
Which Human Resources Are Important for Turning Agritourism Potential into Reality? SWOT Analysis in Rural Nepal
Authors: Bhatta Kumar , Ohe Yasuo , Ciani Adriano
Published: June 2020

Despite Nepal’s agritourism strategies for promoting agrarian villages, agritourism has not yet gained popularity. Based on two different surveys conducted in August 2017 and January 2019, we explore the agritourism development strategies and the agritourism potential for Phikuri village, an agrarian village in Nepal. Using a strengths, weaknesses, opportunities, and threats (SWOT) matrix, we explore four main strategies and 15 sub-strategies. The four main categories are as follows. A maxi-maxi strategy characterised by strengths and opportunities, a maxi-mini strategy characterised by strengths and threats, a mini-maxi strategy characterised by weaknesses and opportunities, and a mini-mini strategy characterised by threats and weaknesses. A quantified SWOT matrix of the estimated total value of the internal and external factor evaluation matrices reveals that Phikuri village has more strengths than weaknesses and more opportunities than threats. We statistically compare the opinions of three different groups, organised by gender, age, and occupation. The results reveal statistically significant differences in the opinions of men and women, younger and older generations, and public and private workers. This study also shows that women are critical for agritourism development, even in a male-dominated society.
Assessing Soil Key Fertility Attributes Using a Portable X-ray Fluorescence: A Simple Method to Overcome Matrix Effect
Authors: Tavares Tiago Rodrigues , Mouazen Abdul Mounem , Alves Elton Eduardo Novais , dos Santos Felipe Rodrigues , Melquiades Fábio Luiz , Pereira de Carvalho Hudson Wallace , Molin José Paulo
Published: June 2020

The matrix effect is one of the challenges to be overcome for a successful analysis of soil samples using X-ray fluorescence (XRF) sensors. This work aimed at evaluation of a simple modeling approach consisted of Compton normalization (CN) and multivariate regressions (e.g., multiple linear regressions (MLR) and partial least squares regression (PLSR)) to overcome the soil matrix effect, and subsequently improve the prediction accuracy of key soil fertility attributes. A portable XRF was used for analyzing 102 soil samples collected from two agricultural fields with contrasting soil matrices. Using the intensity of emission lines as input, preprocessing methods included with and without the CN. Univariate regression models for the prediction of clay, cation exchange capacity (CEC), and exchangeable (ex-) K and Ca were compared with the corresponding MLR models to assess matrix effect mitigation. The MLR and PLSR models improved the prediction results of the univariate models for both preprocessing methods, proving to be promising strategies for mitigating the matrix effect. In turn, the CN also mitigated part of the matrix effect for ex-K, ex-Ca, and CEC predictions, by improving the predictive performance of these elements when used in univariate and multivariate models. The CN has not improved the prediction accuracy of clay. The prediction performances obtained using MLR and PLSR were comparable for all evaluated attributes. The combined use of CN with multivariate regressions (MLR or PLSR) achieved excellent prediction results for CEC (R2 = 0.87), ex-K (R2 ≥ 0.94), and ex-Ca (R2 ≥ 0.96), whereas clay predictions were comparable with and without CN (0.89 ≤ R2 ≤ 0.92). We suggest using multivariate regressions (MLR or PLSR) combined with the CN to remove the soil matrix effects and consequently result in optimal prediction results of the studied key soil fertility attributes. The prediction performance observed for this solution showed comparable results to the approach based on the preprogrammed measurement package tested (Geo Exploration package, Bruker AXS, Madison, WI, USA).
Efficacy of Different Drenching Regimens of Gluconeogenic Precursors during Transition Period on Body Condition Score, Production, Reproductive Performance, Subclinical Ketosis and Economics of Dairy Cows
Authors: El-Kasrawy Nagwa I. , Swelum Ayman A. , Abdel-Latif Mervat A. , Alsenosy Abd El-Wahab A. , Beder Noha A. , Alkahtani Saad , Abdel-Daim Mohamed M. , Abd El-Aziz Ayman H.
Published: June 2020

A total of 108 Holstein cows were used to evaluate the effect of drenching propylene glycol (PG) either individually or in combination with glycerol (G) on body condition score (BCS), production, reproductive performance, protection against subclinical ketosis and economic benefit of dairy cows during the transition period. The six groups (n = 18/group) were; Control group, cows received no treatment; PG300, cows were drenched 300 mL PG for 7 days pre-expected day of calving and 21 days post-calving; PG400, cows were drenched 400 mL PG for 7 days pre-and 7 days post-calving; PG500, cows were drenched 500 mL PG for 10 days pre-and 10 days post-calving; GPG150, cows were drenched a mixture of 150 mL G and 150 mL PG for 21 days pre-and 21 days post-calving; GPG250, cows were drenched a mixture of 250 mL G and 250 mL PG for 21 days pre-and 21 days post-calving. BCS at 30 days in milk (DIM) was significantly higher in all treated groups in comparison to the control one and the best values were observed in GPG250, GPG150 and PG500 groups. Lactation length (LL) was significantly (p < 0.001) shorter in GPG250, GPG150 and PG500 groups than control group. There was a significant increment in 305 milk yield (p < 0.001) and average daily milk yield (p < 0.001) in GPG250 and PG500 groups than other groups except for PG300 with the lowest values for control and PG400. Cows in all treatment groups were observed in heat and conceived earlier as well as had shorter open days and calving interval durations (p < 0.001) and low number of services per conception (p = 0.004) compared to control group with better results for PG500 and GPG250 groups. BHB level and percentage of cows suffered from subclinical ketosis at 7 DIM was significantly lower in GPG250, GPG150 and PG500 groups than control group. Cows in treatment groups had a significantly higher glucose level (p = 0.006) compared to control group. Regarding to economics, the control group showed the highest feed costs, insemination costs and total costs per animal. Additionally, control and PG400 groups had the highest cost per kilogram of milk from total and feed costs compared to other cows. PG300, PG500 and GPG250 groups recorded a greater net return and income over feed cost (IOFC). In conclusion, the continuous drenching of propylene glycol either individually or in combination with glycerol regimens for long duration (PG300, PG500, GPG150, GPG250) during the transition period of dairy cows may reduce the incidence of subclinical ketosis and consequently improve milk production, reproduction and economics compared to drenching for short duration (PG400).
Insight into the Possible Formation Mechanism of the Intersex Phenotype of Lanzhou Fat-Tailed Sheep Using Whole-Genome Resequencing
Authors: Li Jie , Xu Han , Liu Xinfeng , Xu Hongwei , Cai Yong , Lan Xianyong
Published: June 2020

Intersex, also known as hermaphroditism, is a serious hazard to animal husbandry and production. The mechanism of ovine intersex formation is not clear. Therefore, genome-wide resequencing on the only two intersex and two normal Lanzhou fat-tailed (LFT) sheep, an excellent but endangered Chinese indigenous sheep breed, was performed. Herein, the deletion of homologous sequences of the goat polled intersex syndrome (PIS) region (8787 bp, 247747059–247755846) on chromosome 1 of the LFT sheep was not the cause of the ovine intersex trait. By detecting the selective sweep regions, we found that the genes related to androgen biosynthesis and follicle stimulating hormone response items, such as steroid 5 alpha-reductase 2 (SRD5A2), steroid 5 alpha-reductase 3 (SRD5A3), and pro-apoptotic WT1 regulator (PAWR), may be involved in the formation of intersex traits. Furthermore, the copy number variations of the four regions, chr9: 71660801–71662800, chr1: 50776001–50778000, chr4: 58119201–58121600, and chr16: 778801–780800, may affect the expression of the zinc finger protein, FOG family member 2 (ZFPM2), LIM homeobox 8 (LHX8), inner mitochondrial membrane peptidase subunit 2 (IMMP2L) and slit guidance ligand 3 (SLIT3) genes, respectively, which contribute to the appearance of intersex traits. These results may supply a theoretical basis for the timely detection and elimination of intersex individuals in sheep, which could accelerate the healthy development of animal husbandry.
Plasmatic Urea Nitrogen in Growing Rabbits with Different Combinations of Dietary Levels of Lysine, Sulphur Amino Acids and Threonine
Authors: Marín-García Pablo Jesús , López-Luján Mari Carmen , Ródenas Luís , Martínez-Paredes Eugenio Melchor , Blas Enrique , Pascual Juan José
Published: June 2020

A total of 27 experimental diets were formulated starting from the same basal mixture, with a moderate content of crude protein and digestible energy (155 g and 9.86 MJ/kg of digestible matter (DM), respectively, both estimated). The contents of lysine, sulphur amino acids and threonine were variable. The first one, close to the current recommendations (Medium, M; 8.1, 5.8 and 6.9 g/kg DM for lysine, sulphur amino acids and threonine, respectively), and two other levels were on average 15% higher (High, H; 9.4, 6.6 and 7.8 g/kg DM for lysine, sulphur amino acids and threonine, respectively) or lower (Low, L; 6.7, 4.9 and 5.7 g/kg DM for lysine, sulphur amino acids and threonine, respectively). Diets were named with three letters, indicating lysine, sulphur amino acids and threonine levels, respectively. In total, 918 weaned rabbits (28 days old) were used (34 per diet). At weaning, animals were fed ad libitum with a commercial diet until day 46, day 47 each collective cage was randomly switched to one experimental diet. At day 48, blood samples were collected at 08:00h then the animals were subjected to 10 h of fasting and a second blood sample was extracted at 21.00h. At 08:00h, Pasmatic urea nitrogen (PUN) was higher with the L level of lysine (p < 0.001), unaffected by the level of sulphur amino acids and increased with the level of threonine (p < 0.001). At 21:00h, minimum PUN was observed with the MHL diet (14.72 ± 0.661 mg/dL). Taken into account the usual recommendations (established for a diet containing 11.3 MJ DE/kg DM, and then being 0.72, 0.51 and 0.61 g/MJ DE for lysine, sulphur amino acids and threonine, respectively), these results suggest that a diet containing more lysine and sulphur amino acids per energy unit (around 0.82 and 0.67 g/MJ DE) could better fit the growing rabbit requirements, although studies on the effects of such a diet on performance and protein retention are necessary.
Aversion to Desflurane and Isoflurane in Sprague-Dawley Rats (Rattus norvegicus)
Authors: Frost Katrina , Shah Maaria , Leung Vivian S.Y. , Pang Daniel S.J.
Published: June 2020

Carbon dioxide and isoflurane are widely used for killing rats, yet may not truly achieve “euthanasia”, because they elicit aversion. The inhalant anesthetic desflurane is faster acting than isoflurane, representing a potential refinement. Using an aversion-avoidance paradigm, 24 rats were exposed to isoflurane or desflurane (n = 12 per group) at initial exposure. Fourteen rats were then re-exposed to isoflurane or desflurane (n = 7 per group), after a 7 days washout period. Initial exposure: time to recumbency was faster for desflurane than isoflurane (p = 0.0008, 95% CI [-12.9 to 32.6 s]), with 9/12 and 6/12 rats becoming recumbent, respectively. At initial exposure, there was no difference between groups in time to withdrawal (p = 0.714). At re-exposure, all rats withdrew and no rats became recumbent. Time to withdrawal at re-exposure did not differ between treatment groups (p = 0.083). Compared to initial exposure, time to withdrawal during re-exposure was similar for isoflurane (p = 0.228) and faster with desflurane (p = 0.012, 95% CI [19.1 to 49.5 s]). Isoflurane and desflurane are similarly aversive, with aversion increasing at re-exposure. The shorter time from exposure to recumbency with desflurane indicates that any distress is of a shorter duration when compared with isoflurane.
Characteristics and Demography of a Free-Ranging Ethiopian Hedgehog, Paraechinus aethiopicus, Population in Qatar
Authors: Pettett Carly , W. Macdonald David , Al-Hajiri Afra , Al-Jabiry Hayat , Yamaguchi Nobuyuki
Published: June 2020

Information on population characteristics of Paraechinus is valuable for ensuring long term survival of populations, however, studies are currently lacking. Here we investigate the population dynamics of Ethiopian hedgehogs based on a capture-mark-recapture study in Qatar by fitting Jolly-Seber and Cormack-Jolly-Seber models. Over the 19 months of the study, we estimate a mean population of 60 hedgehogs, giving a density of 7 hedgehogs per km2 in our 8.5 km2 search area. The monthly abundance of hedgehogs decreased over the study and although survival was constant over the study period, with a mean monthly rate of 75%, there was a decline in the number of new entrants over time. We also studied these parameters over one year, excluding winter, and found that monthly estimates of juvenile and subadult survival decreased over time. We surmise that survival of juveniles may be a factor in the decrease in abundance and there may be implications for the persistence of this population, with anthropogenic influenced resources playing an important role. We caught between 91.3% and 100% of the estimated population at this site, indicating that our capture methodology was efficient. We conclude that the methodology used here is transferrable to other hedgehog species.
The Effects of a Glucocorticoid Receptor Agonist (GRA) on the Immune Function, Nutrient Digestibility, and Wean-to-Finish Growth Performance of Early-Weaned Pigs
Authors: Wooten Hailey , Kim Hwanhee , Rakhshandeh Amanda R. , Rakhshandeh Anoosh
Published: June 2020

This study assessed the viability of glucocorticoid receptor agonist (GRA) treatment as an alternative to in-feed antibiotics (ANT) in wean-to-finish pigs. A total of 209 piglets were assigned to eight treatments based on a factorial arrangement, with GRA (+ vs. −; dexamethasone, 0.2 mg/kg body weight, BW), ANT (+ vs. −; 110 mg/kg in-feed Tylosin) and sex (gilt vs. barrow) as the main factors. The serial slaughter technique and serial blood collection were performed on 115 pigs during the first week post-weaning to collect blood, tissue and ileal digesta samples. Fecal samples were collected to determine energy digestibility. In comparison to ANT, GRA more effectively improved the measures of systemic inflammation, protein utilization and recovery-associated biomarkers (p ≤ 0.05). Relative to the control group, GRA treatment improved (p ≤ 0.03) dietary nutrient digestibility relative to control pigs, which was comparable to ANT effects. Relative to the control group, all groups had a higher ADG and BW during the starter phase (p < 0.01). Similar to the ANT group, GRA improved the gain-to-feed ratio relative to the control group during the starter phase. Relative to control pigs, overall BW was higher in GRA and ANT pigs during the grow-to-finish phase (p < 0.01). Collectively, these results suggest that GRA injection improves the growth performance of newly weaned pigs by reducing weaning-induced inflammation and improving nutrient digestibility. GRA can be used as an alternative to in-feed ANT to mitigate the effects of weaning stress on pigs.
Influence of Dietary Supplementation of Salix alba Bark on Performance, Oxidative Stress Parameters in Liver and Gut Microflora of Broilers
Authors: Panaite Tatiana Dumitra , Saracila Mihaela , Papuc Camelia Puia , Predescu Corina Nicoleta , Soica Cristina
Published: June 2020

The paper aimed to analyse the effect of dietary Salix alba L. bark powder (SAB) on broiler performance in terms of oxidative stress parameters in liver and gut microflora. One hundred and eighty Cobb 500 broiler chicks (14 days) were allotted to three homogeneous treatments (SAB 0%; SAB 0.025%; SAB 0.05%). The broilers were housed in an environmentally controlled space (10 replicates, six broilers/replicate). Compared to dietary control treatment (SAB 0%), the other treatments included 0.025% SAB (SAB 0.025%) and 0.05% SAB (SAB 0.05%). The results showed that SAB powder used in broiler diet had a high total phenolic content. Regarding the performance results, significant differences between experimental and control treatments were recorded only for average daily feed intake (35–42 days). The broilers fed with SAB powder had a significantly lower hepatic level of malondialdehide and glutathione, a higher total antioxidant capacity than those fed control treatment, and demonstrated a positive effect on the development of non-pathogenic bacteria (lactobacilli) but a decrease in the population of pathogenic ones (E. coli, staphylococci). Our findings suggested that dietary 0.05% SAB powder could be an effective solution to impede the oxidative stress in broiler liver and to improve gut microflora.
Evidence for Individual Differences in Behaviour and for Behavioural Syndromes in Adult Shelter Cats
Authors: Martínez-Byer Sandra , Urrutia Andrea , Szenczi Péter , Hudson Robyn , Bánszegi Oxána
Published: June 2020

Consistent inter-individual differences in behaviour have been previously reported in adult shelter cats. In this study, we aimed to assess whether repeatable individual differences in behaviours exhibited by shelter cats in different situations were interrelated, forming behavioural syndromes. We tested 31 adult cats in five different behavioural tests, repeated three times each: a struggle test where an experimenter restrained the cat, a separation/confinement test where the cat spent 2 min in a pet carrier, a mouse test where the cat was presented with a live mouse in a jar, and two tests where the cat reacted to an unfamiliar human who remained either passive or actively approached the cat. Individual differences in behaviour were consistent (repeatable) across repeated trials for each of the tests. We also found associations between some of the behaviours shown in the different tests, several of which appeared to be due to differences in human-oriented behaviours. This study is the first to assess the presence of behavioural syndromes using repeated behavioural tests in different situations common in the daily life of a cat, and which may prove useful in improving the match between prospective owner and cat in shelter adoption programmes.
An Environmentally Friendly Practice Used in Olive Cultivation Capable of Increasing Commercial Interest in Waste Products from Oil Processing
Authors: Dini Irene , Graziani Giulia , Fedele Francalisa Luisa , Sicari Andrea , Vinale Francesco , Castaldo Luigi , Ritieni Alberto
Published: June 2020

In the Rural Development Plan (2014–2020), the European Commission encouraged the conversion and supported the maintenance of organic farming. Organic olive oil (bioEVOO) production involves the use of environmentally sustainable fertilizers and the recycling of olive pomace (Pom) and olive vegetation waters (VW) to reduce the environmental impact of these wastes. An ecofriendly way to recycle olive wastes is to reuse them to extract bioactive compounds. In this study, the total phenolic compounds content, their profile and dosage, the antioxidant action in oil, pomace, and vegetation water was evaluated when the Trichoderma harzianum M10 was used as a biostimulant in agriculture. Two spectrophotometric tests (2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic) acid (ABTS)) evaluated the antioxidant potential of samples, a spectrophotometric method estimated total phenolic content, and an Ultra-High-Performance Liquid Chromatography (UHPLC)–Orbitrap method evaluated the phenolics profile. Our results showed that the biostimulation improved the antioxidant potential and the total concentration of phenolics in the bioEVOO and bio-pomace (bioPom) samples and mainly enhanced, among all classes of phenolic compounds, the production of the flavonoids and the secoiridoids. Moreover, they demonstrated the Trichoderma action in the mevalonate pathway to produce phenols for the first time. The decisive action of the Thricoderma on the production of phenolic compounds increases the economic value of the waste materials as a source of bioactive compounds useful for the pharmaceutical, cosmetic, and food industries.
Anti-Migratory Effects of 4′-Geranyloxyferulic Acid on LPS-Stimulated U937 and HCT116 Cells via MMP-9 Down-Regulation: Involvement of ROS/ERK Signaling Pathway
Authors: Franceschelli Sara , Gatta Daniela Maria Pia , Ferrone Alessio , Mezza Giulia , Speranza Lorenza , Pesce Mirko , Grilli Alfredo , Gallorini Marialucia , Giacomo Viviana di , Ghinassi Barbara , Fiorito Serena , Genovese Salvatore , Ricciotti Emanuela , Felaco Mario , Patruno Antonia
Published: June 2020

Matrix metalloproteinases (MMPs) play a crucial role in tumor angiogenesis, and metastasis. 4′-geranyloxyferulic acid (GOFA) has anti-tumor and anti-inflammatory proprieties. Herein, we aimed to determine whether this compound affects cell survival, invasion, and migration through reactive oxygen species (ROS)-mediated MMPs activation of extracellular signal-regulated kinases (ERKs) and p38 signaling in lymphocytic histiocytoma (U937) and colorectal cancer (HCT116) cells. We observed that lipopolysaccharide (LPS) stimulated U937 and HCT116 cells presented abnormal cell proliferation and increased metalloproteinase (MMP-9) activity and expression. Non-cytotoxic doses of GOFA blunted matrix invasive potential by reducing LPS-induced MMP-9 expression and cell migration via inhibiting ROS/ ERK pathway. GOFA also attenuated apoptosis and cell senescence. Our findings indicate that GOFA, inhibiting cancer cell proliferation and migration, could be therapeutically beneficial to prevent tumor metastasis.
An Update on Mitochondrial Reactive Oxygen Species Production
Authors: Mailloux Ryan J.
Published: June 2020

Mitochondria are quantifiably the most important sources of superoxide (O2●−) and hydrogen peroxide (H2O2) in mammalian cells. The overproduction of these molecules has been studied mostly in the contexts of the pathogenesis of human diseases and aging. However, controlled bursts in mitochondrial ROS production, most notably H2O2, also plays a vital role in the transmission of cellular information. Striking a balance between utilizing H2O2 in second messaging whilst avoiding its deleterious effects requires the use of sophisticated feedback control and H2O2 degrading mechanisms. Mitochondria are enriched with H2O2 degrading enzymes to desensitize redox signals. These organelles also use a series of negative feedback loops, such as proton leaks or protein S-glutathionylation, to inhibit H2O2 production. Understanding how mitochondria produce ROS is also important for comprehending how these organelles use H2O2 in eustress signaling. Indeed, twelve different enzymes associated with nutrient metabolism and oxidative phosphorylation (OXPHOS) can serve as important ROS sources. This includes several flavoproteins and respiratory complexes I-III. Progress in understanding how mitochondria generate H2O2 for signaling must also account for critical physiological factors that strongly influence ROS production, such as sex differences and genetic variances in genes encoding antioxidants and proteins involved in mitochondrial bioenergetics. In the present review, I provide an updated view on how mitochondria budget cellular H2O2 production. These discussions will focus on the potential addition of two acyl-CoA dehydrogenases to the list of ROS generators and the impact of important phenotypic and physiological factors such as tissue type, mouse strain, and sex on production by these individual sites.
Special Issue: Denitrification in Agricultural Soils
Authors: Mastrocicco Micòl
Published: June 2020

Denitrification, the main microbial reduction process of nitrate to the inert dinitrogen gas or to reactive gases such as nitric oxide and nitrous oxide [...]
A Machine Learning Approach to Predict an Early Biochemical Recurrence after a Radical Prostatectomy
Authors: Park Seongkeun , Byun Jieun , Woo Ji young
Published: June 2020

Background: Approximately 20–50% of prostate cancer patients experience biochemical recurrence (BCR) after radical prostatectomy (RP). Among them, cancer recurrence occurs in about 20–30%. Thus, we aim to reveal the utility of machine learning algorithms for the prediction of early BCR after RP. Methods: A total of 104 prostate cancer patients who underwent magnetic resonance imaging and RP were evaluated. Four well-known machine learning algorithms (i.e., k-nearest neighbors (KNN), multilayer perceptron (MLP), decision tree (DT), and auto-encoder) were applied to build a prediction model for early BCR using preoperative clinical and imaging and postoperative pathologic data. The sensitivity, specificity, and accuracy for detection of early BCR of each algorithm were evaluated. Area under the receiver operating characteristics (AUROC) analyses were conducted. Results: A prediction model using an auto-encoder showed the highest prediction ability of early BCR after RP using all data as input (AUC = 0.638) and only preoperative clinical and imaging data (AUC = 0.656), followed by MLP (AUC = 0.607 and 0.598), KNN (AUC = 0.596 and 0.571), and DT (AUC = 0.534 and 0.495). Conclusion: The auto-encoder-based prediction system has the potential for accurate detection of early BCR and could be useful for long-term follow-up planning in prostate cancer patients after RP.
A New Formulation for Predicting the Collision Damage of Steel Stiffened Cylinders Subjected to Dynamic Lateral Mass Impact
Authors: Do Quang Thang , Huynh Van Vu , Vu Mai The , Tuyen Vu Van , Pham-Thanh Nhut , Tra Tran Hung , Vu Quang-Viet , Cho Sang-Rai
Published: June 2020

The objective of this study is to develop a new formulation for predicting the permanent local denting damage of steel ring and/or stringer-stiffened cylinders under dynamic lateral mass impact. The considered scenarios could represent the collisions of offshore cylindrical structures with bow or stern of service vessels or floating objects. Before deriving the formulations, the numerical methods were developed using ABAQUS/Explicit to determine the deformation of these stiffened cylinder structures subjected to dynamic lateral mass impact. Next, rigorous parametric studies were performed on the actual design full-scaled stiffened cylinder examples using the developed numerical method. Based on the rigorous numerical results, new simple design formulations to predict the maximum permanent local dent depth of a stiffened cylinder are derived through a regression study as the function of a non-dimensional energy parameter. The accuracy and reliability of the derived formulations are confirmed by comparison with the available test results, nonlinear FEA and existing analytical, and empirical equations in the literature. A good agreement with existing test data for ship-offshore structure collisions was achieved.
Boosting of Denoising Effect with Fusion Strategy
Authors: Yang Fangjia , Xu Shaoping , Li Chongxi
Published: June 2020

Image denoising, a fundamental step in image processing, has been widely studied for several decades. Denoising methods can be classified as internal or external depending on whether they exploit the internal prior or the external noisy-clean image priors to reconstruct a latent image. Typically, these two kinds of methods have their respective merits and demerits. Using a single denoising model to improve existing methods remains a challenge. In this paper, we propose a method for boosting the denoising effect via the image fusion strategy. This study aims to boost the performance of two typical denoising methods, the nonlocally centralized sparse representation (NCSR) and residual learning of deep CNN (DnCNN). These two methods have complementary strengths and can be chosen to represent internal and external denoising methods, respectively. The boosting process is formulated as an adaptive weight-based image fusion problem by preserving the details for the initial denoised images output by the NCSR and the DnCNN. Specifically, we design two kinds of weights to adaptively reflect the influence of the pixel intensity changes and the global gradient of the initial denoised images. A linear combination of these two kinds of weights determines the final weight. The initial denoised images are integrated into the fusion framework to achieve our denoising results. Extensive experiments show that the proposed method significantly outperforms the NCSR and the DnCNN both quantitatively and visually when they are considered as individual methods; similarly, it outperforms several other state-of-the-art denoising methods.
Performance Comparisons of Bio-Micro Genetic Algorithms on Robot Locomotion
Authors: Chávez-Estrada Francisco A. , Sandoval-Gutiérrez Jacobo , Herrera-Lozada Juan C. , Olguín-Carbajal Mauricio , Martínez-Vázquez Daniel L. , Hernández-Bolaños Miguel , Rivera-Zárate Israel
Published: June 2020

This paper presents a comparison of four algorithms and identifies the better one in terms of convergence to the best performance for the locomotion of a quadruped robot designed. Three algorithms found in the literature review: a standard Genetic Algorithm (GA), a micro-Genetic Algorithm ( μ GA), and a micro-Artificial Immune System ( μ AIS); the fourth algorithm is a novel micro-segmented Genetic Algorithm ( μ sGA). This research shows how the computing time affects the performance in different algorithms of the gait on the robot physically; this contribution complements other studies that are limited to simulation. The μ sGA algorithm uses less computing time since the individual is segmented into specific bytes. In contrast, the use of a computer and the high demand in computational resources for the GA are avoided. The results show that the performance of μ sGA is better than the other three algorithms (GA, μ GA and μ AIS). The quadruped robot prototype guarantees the same conditions for each test. The structure of the platform was developed by 3D printing. This structure was used to accommodate the mechanisms, sensors and servomechanisms as actuators. It also has an internal battery and a multicore Embedded System (mES) to process and control the robot locomotion. The computing time was reduced using an mES architecture that enables parallel processing, meaning that the requirements for resources and memory were reduced. For example, in the experiment of a one-second gait cycle, GA uses 700% of computing time, μ GA (76%), μ AIS (32%) and μ sGA (13%). This research solves the problem of quadruped robot’s locomotion and gives a feasible solution (Central Pattern Generators, (CPGs)) with real performance parameters using a μ sGA bio-micro algorithm and a mES architecture.
Naturally Occurring and Artificial N9-Cytokinin Conjugates: From Synthesis to Biological Activity and Back
Authors: Vylíčilová Hana , Bryksová Magdaléna , Matušková Vlasta , Doležal Karel , Plíhalová Lucie , Strnad Miroslav
Published: June 2020

Cytokinins and their sugar or non-sugar conjugates are very active growth-promoting factors in plants, although they occur at very low concentrations. These compounds have been identified in numerous plant species. This review predominantly focuses on 9-substituted adenine-based cytokinin conjugates, both artificial and endogenous, sugar and non-sugar, and their roles in plants. Acquired information about their biological activities, interconversions, and metabolism improves understanding of their mechanisms of action and functions in planta. Although a number of 9-substituted cytokinins occur endogenously, many have also been prepared in laboratories to facilitate the clarification of their physiological roles and the determination of their biological properties. Here, we chart advances in knowledge of 9-substituted cytokinin conjugates from their discovery to current understanding and reciprocal interactions between biological properties and associated structural motifs. Current organic chemistry enables preparation of derivatives with better biological properties, such as improved anti-senescence, strong cell division stimulation, shoot forming, or more persistent stress tolerance compared to endogenous or canonical cytokinins. Many artificial cytokinin conjugates stimulate higher mass production than naturally occurring cytokinins, improve rooting, or simply have high stability or bioavailability. Thus, knowledge of the biosynthesis, metabolism, and activity of 9-substituted cytokinins in various plant species extends the scope for exploiting both natural and artificially prepared cytokinins in plant biotechnology, tissue culture, and agriculture.
The Architectural Dynamics of the Bacterial Flagellar Motor Switch
Authors: Khan Shahid
Published: June 2020

The rotary bacterial flagellar motor is remarkable in biochemistry for its highly synchronized operation and amplification during switching of rotation sense. The motor is part of the flagellar basal body, a complex multi-protein assembly. Sensory and energy transduction depends on a core of six proteins that are adapted in different species to adjust torque and produce diverse switches. Motor response to chemotactic and environmental stimuli is driven by interactions of the core with small signal proteins. The initial protein interactions are propagated across a multi-subunit cytoplasmic ring to switch torque. Torque reversal triggers structural transitions in the flagellar filament to change motile behavior. Subtle variations in the core components invert or block switch operation. The mechanics of the flagellar switch have been studied with multiple approaches, from protein dynamics to single molecule and cell biophysics. The architecture, driven by recent advances in electron cryo-microscopy, is available for several species. Computational methods have correlated structure with genetic and biochemical databases. The design principles underlying the basis of switch ultra-sensitivity and its dependence on motor torque remain elusive, but tantalizing clues have emerged. This review aims to consolidate recent knowledge into a unified platform that can inspire new research strategies.
Green Synthesis of Gold and Silver Nanoparticles Using Leaf Extract of Clerodendrum inerme; Characterization, Antimicrobial, and Antioxidant Activities
Authors: Khan Shakeel Ahmad , Shahid Sammia , Lee Chun-Sing
Published: June 2020

Due to their versatile applications, gold (Au) and silver (Ag) nanoparticles (NPs) have been synthesized by many approaches, including green processes using plant extracts for reducing metal ions. In this work, we propose to use plant extract with active biomedical components for NPs synthesis, aiming to obtain NPs inheriting the biomedical functions of the plants. By using leaves extract of Clerodendrum inerme (C. inerme) as both a reducing agent and a capping agent, we have synthesized gold (CI-Au) and silver (CI-Ag) NPs covered with biomedically active functional groups from C. inerme. The synthesized NPs were evaluated for different biological activities such as antibacterial and antimycotic against different pathogenic microbes (B. subtilis, S. aureus, Klebsiella, and E. coli) and (A. niger, T. harzianum, and A. flavus), respectively, using agar well diffusion assays. The antimicrobial propensity of NPs further assessed by reactive oxygen species (ROS) glutathione (GSH) and FTIR analysis. Biofilm inhibition activity was also carried out using colorimetric assays. The antioxidant and cytotoxic potential of CI-Au and CI-Ag NPs was determined using DPPH free radical scavenging and MTT assay, respectively. The CI-Au and CI-Ag NPs were demonstrated to have much better antioxidant in terms of %DPPH scavenging (75.85% ± 0.67% and 78.87% ± 0.19%), respectively. They exhibited excellent antibacterial, antimycotic, biofilm inhibition and cytotoxic performance against pathogenic microbes and MCF-7 cells compared to commercial Au and Ag NPs functionalized with dodecanethiol and PVP, respectively. The biocompatibility test further corroborated that CI-Ag and CI-Au NPs are more biocompatible at the concentration level of 1–50 µM. Hence, this work opens a new environmentally-friendly path for synthesizing nanomaterials inherited with enhanced and/or additional biomedical functionalities inherited from their herbal sources.
Heme-Oxygenase and Kidney Transplantation: A Potential for Target Therapy?
Authors: Corona Daniela , Ekser Burcin , Gioco Rossella , Caruso Massimo , Schipa Chiara , Veroux Pierfrancesco , Giaquinta Alessia , Granata Antonio , Veroux Massimiliano
Published: June 2020

Kidney transplantation is a well-established therapy for patients with end-stage renal disease. While a significant improvement of short-term results has been achieved in the short-term, similar results were not reported in the long-term. Heme-oxygenase (HO) is the rate-limiting enzyme in heme catabolism, converting heme to iron, carbon monoxide, and biliverdin. Heme-oxygenase overexpression may be observed in all phases of transplant processes, including brain death, recipient management, and acute and chronic rejection. HO induction has been proved to provide a significant reduction of inflammatory response and a reduction of ischemia and reperfusion injury in organ transplantation, as well as providing a reduction of incidence of acute rejection. In this review, we will summarize data on HO and kidney transplantation, suggesting possible clinical applications in the near future to improve the long-term outcomes.
Pseudouridine Synthase RsuA Captures an Assembly Intermediate That Is Stabilized by Ribosomal Protein S17
Authors: Jayalath Kumudie , Frisbie Sean , To Minhchau , Abeysirigunawardena Sanjaya
Published: June 2020

The ribosome is a large ribonucleoprotein complex that synthesizes protein in all living organisms. Ribosome biogenesis is a complex process that requires synchronization of various cellular events, including ribosomal RNA (rRNA) transcription, ribosome assembly, and processing and post-transcriptional modification of rRNA. Ribosome biogenesis is fine-tuned with various assembly factors, possibly including nucleotide modification enzymes. Ribosomal small subunit pseudouridine synthase A (RsuA) pseudouridylates U516 of 16S helix 18. Protein RsuA is a multi-domain protein that contains the N-terminal peripheral domain, which is structurally similar to the ribosomal protein S4. Our study shows RsuA preferably binds and pseudouridylates an assembly intermediate that is stabilized by ribosomal protein S17 over the native-like complex. In addition, the N-terminal domain truncated RsuA showed that the presence of the S4-like domain is important for RsuA substrate recognition.
The S100B Protein and Partners in Adipocyte Response to Cold Stress and Adaptive Thermogenesis: Facts, Hypotheses, and Perspectives
Authors: Baudier Jacques , Gentil Benoit J
Published: June 2020

In mammals, adipose tissue is an active secretory tissue that responds to mild hypothermia and as such is a genuine model to study molecular and cellular adaptive responses to cold-stress. A recent study identified a mammal-specific protein of the endoplasmic reticulum that is strongly induced in the inguinal subcutaneous white adipocyte upon exposure to cold, calsyntenin 3β (CLSTN3β). CLSTN3β regulates sympathetic innervation of thermogenic adipocytes and contributes to adaptive non-shivering thermogenesis. The calcium- and zinc-binding S100B is a downstream effector in the CLSTN3β pathways. We review, here, the literature on the transcriptional regulation of the S100b gene in adipocyte cells. We also rationalize the interactions of the S100B protein with its recognized or hypothesized intracellular (p53, ATAD3A, CYP2E1, AHNAK) and extracellular (Receptor for Advanced Glycation End products (RAGE), RPTPσ) target proteins in the context of adipocyte differentiation and adaptive thermogenesis. We highlight a chaperon-associated function for the intracellular S100B and point to functional synergies between the different intracellular S100B target proteins. A model of non-classical S100B secretion involving AHNAK/S100A10/annexin2-dependent exocytosis by the mean of exosomes is also proposed. Implications for related areas of research are noted and suggestions for future research are offered.
Probiotics-Containing Yogurt Ingestion and H. pylori Eradication Can Restore Fecal Faecalibacterium prausnitzii Dysbiosis in H. pylori-Infected Children
Authors: Yang Yao-Jong , Chen Peng-Chieh , Lai Fu-Ping , Tsai Pei-Jane , Sheu Bor-Shyang
Published: June 2020

This study investigated the compositional differences in fecal microbiota between children with and without H. pylori infection and tested whether probiotics-containing yogurt and bacterial eradication improve H. pylori-related dysbiosis. Ten H. pylori-infected children and 10 controls ingested probiotics-containing yogurt for 4 weeks. Ten-day triple therapy plus yogurt was given to the infected children on the 4th week. Fecal samples were collected at enrollment, after yogurt ingestion, and 4 weeks after successful H. pylori eradication for cytokines and microbiota analysis using ELISA and metagenomic sequencing of the V4 region of the 16S rRNA gene, respectively. The results showed H. pylori-infected children had significantly higher levels of fecal TGF-β1 than those who were not infected. Eight of 295 significantly altered OTUs in the H. pylori-infected children were identified. Among them, the abundance of F. prausnitzii was significantly lower in the H. pylori-infected children, and then increased after yogurt ingestion and successful bacterial eradication. We further confirmed probiotics promoted F. prausnitzii growth in vitro and in ex vivo using real-time PCR. Moreover, F. prausnitzii supernatant significantly ameliorated lipopolysaccharide-induced IL-8 in HT-29 cells. In conclusions, Probiotics-containing yogurt ingestion and H. pylori eradication can restore the decrease of fecal F. prausnitzii in H. pylori-infected children.
The Pivotal Role of the Dysregulation of Cholesterol Homeostasis in Cancer: Implications for Therapeutic Targets
Authors: Mok Etienne Ho Kit , Lee Terence Kin Wah
Published: June 2020

Cholesterol plays an important role in cellular homeostasis by maintaining the rigidity of cell membranes, providing a medium for signaling transduction, and being converted into other vital macromolecules, such as sterol hormones and bile acids. Epidemiological studies have shown the correlation between cholesterol content and cancer incidence worldwide. Accumulating evidence has shown the emerging roles of the dysregulation of cholesterol metabolism in cancer development. More specifically, recent reports have shown the distinct role of cholesterol in the suppression of immune cells, regulation of cell survival, and modulation of cancer stem cells in cancer. Here, we provide a comprehensive review of the epidemiological analysis, functional roles, and mechanistic action of cholesterol homeostasis in regard to its contribution to cancer development. Based on the existing data, cholesterol homeostasis is identified to be a new key player in cancer pathogenesis. Lastly, we also discuss the therapeutic implications of natural compounds and cholesterol-lowering drugs in cancer prevention and treatment. In conclusion, intervention in cholesterol metabolism may offer a new therapeutic avenue for cancer treatment.
Inflammatory Cytokines and ctDNA Are Biomarkers for Progression in Advanced-Stage Melanoma Patients Receiving Checkpoint Inhibitors
Authors: Pedersen Jesper Geert , Madsen Anne Tranberg , Gammelgaard Kristine Raaby , Aggerholm-Pedersen Ninna , Sørensen Boe Sandahl , Øllegaard Trine Heide , Jakobsen Martin Roelsgaard
Published: June 2020

Purpose: Checkpoint inhibitors have significantly improved treatment of metastatic melanoma. However, 40–60% of patients do not respond to therapy, emphasizing the need for better predictive biomarkers for treatment response to immune checkpoint inhibitors. Prorammed death-ligand 1(PD-L1) expression in tumor cells is currently used as a predictive biomarker; however, it lacks specificity. Therefore, it is of utmost importance to identify other novel biomarkers that can predict treatment outcome. Experimental design: We studied a small cohort of 16 patients with advanced-stage melanoma treated with first-line checkpoint inhibitors. Plasma samples were collected prior to treatment initiation and continuously during the first year of treatment. Circulating tumor DNA (ctDNA) level and the expression of ten inflammatory cytokines were analyzed. Results: We found that the ctDNA-level in a blood sample collected after 6–8 weeks of therapy is predictive for response to checkpoint inhibitors. Patients with undetectable ctDNA had significantly longer progression-free survival (PFS) compared with patients with detectable ctDNA (median 26.3 vs. 2.1 months, p = 0.006). In parallel, we identified that high levels of the cytokines monocyte chemoattractant protein 1 (MCP1) and tumor necrosis factor α(TNFα) in baseline blood samples were significantly associated with longer PFS compared to low level of these cytokines (median not reached vs. 8.2 months p = 0.0008). Conclusions: These findings suggest that the levels of ctDNA, MCP1, and TNFα in baseline and early follow-up samples can predict disease progression in metastatic melanoma patients treated with checkpoint inhibitors. Potentially, these minimally invasive biomarkers may identify responders from non-responders.
Lipid Metabolism in Development and Progression of Hepatocellular Carcinoma
Authors: Sangineto Moris , Villani Rosanna , Cavallone Francesco , Romano Antonino , Loizzi Domenico , Serviddio Gaetano
Published: June 2020

Metabolic reprogramming is critically involved in the development and progression of cancer. In particular, lipid metabolism has been investigated as a source of energy, micro-environmental adaptation, and cell signalling in neoplastic cells. However, the specific role of lipid metabolism dysregulation in hepatocellular carcinoma (HCC) has not been widely described yet. Alterations in fatty acid synthesis, β-oxidation, and cellular lipidic composition contribute to initiation and progression of HCC. The aim of this review is to elucidate the mechanisms by which lipid metabolism is involved in hepatocarcinogenesis and tumour adaptation to different conditions, focusing on the transcriptional aberrations with new insights in lipidomics and lipid zonation. This will help detect new putative therapeutic approaches in the second most frequent cause of cancer-related death.