bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of continued folic acid supplementation beyond the first trimester of pregnancy on cognitive performance in the child: a follow-up study from a randomized controlled trial (FASSTT Offspring Trial)
Authors: McNulty Helene , Rollins Mark , Cassidy Tony , Caffrey Aoife , Marshall Barry , Dornan James , McLaughlin Marian , McNulty Breige A. , Ward Mary , Strain J. J. , Molloy Anne M. , Lees-Murdock Diane J. , Walsh Colum P. , Pentieva Kristina
Published: October 2019

Background

Periconceptional folic acid prevents neural tube defects (NTDs), but it is uncertain whether there are benefits for offspring neurodevelopment arising from continued maternal folic acid supplementation beyond the first trimester. We investigated the effect of folic acid supplementation during trimesters 2 and 3 of pregnancy on cognitive performance in the child.

Methods

We followed up the children of mothers who had participated in a randomized controlled trial in 2006/2007 of Folic Acid Supplementation during the Second and Third Trimesters (FASSTT) and received 400 μg/d folic acid or placebo from the 14th gestational week until the end of pregnancy. Cognitive performance of children at 7 years was evaluated using the Wechsler Preschool and Primary Scale of Intelligence (WPPSI-III) and at 3 years using the Bayley’s Scale of Infant and Toddler Development (BSITD-III).

Results

From a total of 119 potential mother-child pairs, 70 children completed the assessment at age 7 years, and 39 at age 3 years. At 7 years, the children of folic acid treated mothers scored significantly higher than the placebo group in word reasoning: mean 13.3 (95% CI 12.4–14.2) versus 11.9 (95% CI 11.0–12.8);p = 0.027; at 3 years, they scored significantly higher in cognition: 10.3 (95% CI 9.3–11.3) versus 9.5 (95% CI 8.8–10.2);p = 0.040. At both time points, greater proportions of children from folic acid treated mothers compared with placebo had cognitive scores above the median values of 10 (girls and boys) for the BSITD-III, and 24.5 (girls) and 21.5 (boys) for the WPPSI-III tests. When compared with a nationally representative sample of British children at 7 years, WPPSI-III test scores were higher in children from folic acid treated mothers for verbal IQ (p < 0.001), performance IQ (p = 0.035), general language (p = 0.002), and full scale IQ (p = 0.001), whereas comparison of the placebo group with British children showed smaller differences in scores for verbal IQ (p = 0.034) and full scale IQ (p = 0.017) and no differences for performance IQ or general language.

Conclusions

Continued folic acid supplementation in pregnancy beyond the early period recommended to prevent NTD may have beneficial effects on child cognitive development. Further randomized trials in pregnancy with follow-up in childhood are warranted.

Trial registration

ISRCTNISRCTN19917787. Registered 15 May 2013.
Acute peripheral immune activation alters cytokine expression and glial activation in the early postnatal rat brain
Authors: Bruce Matthew , Streifel Karin M. , Boosalis Casey A. , Heuer Luke , González Eduardo A. , Li Shuyang , Harvey Danielle J. , Lein Pamela J. , Van de Water Judy
Published: October 2019

Background

Neuroinflammation can modulate brain development; however, the influence of an acute peripheral immune challenge on neuroinflammatory responses in the early postnatal brain is not well characterized. To address this gap in knowledge, we evaluated the peripheral and central nervous system (CNS) immune responses to a mixed immune challenge in early postnatal rats of varying strains and sex.

Methods

On postnatal day 10 (P10), male and female Lewis and Brown Norway rats were injected intramuscularly with either a mix of bacterial and viral components in adjuvant, adjuvant-only, or saline. Immune responses were evaluated at 2 and 5 days post-challenge. Cytokine and chemokine levels were evaluated in serum and in multiple brain regions using a Luminex multiplex assay. Multi-factor ANOVAs were used to compare analyte levels across treatment groups within strain, sex, and day of sample collection. Numbers and activation status of astrocytes and microglia were also analyzed in the cortex and hippocampus by quantifying immunoreactivity for GFAP, IBA-1, and CD68 in fixed brain slices. Immunohistochemical data were analyzed using a mixed-model regression analysis.

Results

Acute peripheral immune challenge differentially altered cytokine and chemokine levels in the serum versus the brain. Within the brain, the cytokine and chemokine response varied between strains, sexes, and days post-challenge. Main findings included differences in T helper (Th) type cytokine responses in various brain regions, particularly the cortex, with respect to IL-4, IL-10, and IL-17 levels. Additionally, peripheral immune challenge altered GFAP and IBA-1 immunoreactivity in the brain in a strain- and sex-dependent manner.

Conclusions

These findings indicate that genetic background and sex influence the CNS response to an acute peripheral immune challenge during early postnatal development. Additionally, these data reinforce that the developmental time point during which the challenge occurs has a distinct effect on the activation of CNS-resident cells.
A pilot and feasibility study of a cognitive behavioural therapy-based anxiety prevention programme for junior high school students in Japan: a quasi-experimental study
Authors: Ohira Ikuyo , Urao Yuko , Sato Yasunori , Ohtani Toshiyuki , Shimizu Eiji
Published: October 2019

Background

There is a good deal of evidence that cognitive behavioural therapy is effective for children and adolescents with anxiety-related problems. In Japan, an anxiety prevention programme based on cognitive behavioural therapy called ‘Journey of the Brave’ has been developed, and it has been demonstrated to be effective for elementary school students (aged 10–11 years). The purpose of this study was to have classroom teachers deliver the programme to junior high school students (aged 12–13 years) and to test the feasibility and efficacy of the programme in this setting.

Methods

This study was a prospective observational study and was approved by the Chiba University Review Board. An intervention group consisting of six classes of students in their first year of junior high school at two different schools (n = 149; 81 boys, 68 girls) received seven 50-min programme sessions. Participants in the control group were recruited from four classes of students in their second year of junior high school at one school (n = 89; 51 boys, 38 girls). All participants completed the Spence Children’s Anxiety Scale at pre-test, post-test, and 2–3 month follow-up. Statistical analysis was conducted using a mixed-effects model for repeated measures model.

Results

Mean total anxiety scores indicated a non-significant decrease at the 2–3 month follow-up for the intervention group compared to the control group. The group differences on the SCAS from baseline to post-test was − .71 (95% CI − 2.48 to 1.06,p = .43), and the 2–3 month follow-up was − .49 (95% CI − 2.60 to 1.61,p = .64).

Conclusions

In this pilot study, implementation of the programme confirmed the partial feasibility of the programme but did not elicit a significant reduction in anxiety scores. In addition, there are several methodological limitations to this study. In the future, we propose to test the feasibility and efficacy of the programme with the required sample size and by comparing groups with equal characteristics as well as by carrying out additional follow-up assessments.

Trial registrationUMIN000032517.
CircWHSC1 promotes ovarian cancer progression by regulating MUC1 and hTERT through sponging miR-145 and miR-1182
Authors: Zong Zhi-Hong , Du Yu-Ping , Guan Xue , Chen Shuo , Zhao Yang
Published: October 2019

Background

Circular RNAs are key regulators in human cancers, however, there is a lack of studies on circRNAs’ specific functions in ovarian cancer.

Methods

Our study used qRT-PCR to detect the differentially expressed circRNAs between normal ovaries and ovarian cancer tissues. Cell function experiments were performed to verify the role of overexpression and silence of circWHSC1, including MTT assay, cell apoptosis assay, wound healing and Matrigel-coated Transwell assay. In vivo tumorigenesis model was constructed by subcutaneous injection in nude mice. Bioinformatics analysis predicted the possible binding sites of circWHSC1 with miRNAs, and confirmed with dual-luciferase reporter assay and RNA pull-down assay. The exosomes were extracted with ultracentrifugation. HE staining was also used to detect morphology of nude mice peritoneum.

Results

We found that circWHSC1 was up-regulated in ovarian cancer tissues, and circWHSC1 expression was higher in moderate & poor differentiation ovarian cancer tissues than in well differentiation ovarian cancer tissues. Overexpression of circWHSC1 increased cell proliferation, migration and invasion, and inhibited cell apoptosis. Silence of circWHSC1 exerted the opposite effects. Additionally, circWHSC1 could sponge miR-145 and miR-1182 and up-regulate the expression of downstream targets MUC1 and hTERT. Exosomal circWHSC1 can be transferred to peritoneal mesothelial cells and promotes peritoneal dissemination.

Conclusions

Our study demonstrates the highly expressed circWHSC1 in ovarian cancer promotes tumorigenesis by sponging miR-145 and miR-1182, and its exosome forms induce tumor metastasis through acting on peritoneal mesothelium.
Correction to: Wogonoside inhibits invasion and migration through suppressing TRAF2/4 expression in breast cancer
Authors: Yao Yuyuan , Zhao Kai , Yu Zhou , Ren Haochuan , Zhao Li , Li Zhiyu , Guo Qinglong , Lu Na
Published: October 2019

In the original publication of this article [1], there are mistakes in Fig. 3c and Fig. 3e.
Direct analysis of volatile components from intact jujube by carbon fiber ionization mass spectrometry
Authors: Sun Shihao , Zhang Yihan , Li Peng , Xi Hui , Wu Lei , Zhang Jianxun , Peng Guixin , Su Yue
Published: October 2019

In situ analysis of odor is an important approach to connect odor with chemical composition. However, it is difficult to conduct a rapid direct analysis of the odor sample because of low analyte concentration and sampling. To achieve the direct analysis, a carbon fiber ionization mass spectrometry (CFI-MS) method has been developed and applied to measure volatile components releasing from intact jujube. To build the CFI source, a 2.0-cm long carbon fiber bundle was integrated on the pin of a commercial corona discharge needle by mean of a 1.3-cm long stainless hollow tube. Odor sample driven by N2 gas can be directly introduced to the carbon fiber bundle to complete the ionization of analytes. Acetic acid, ethyl acetate, ethyl caproate, octyl acetate, and damascone present in jujube were selected to evaluate the performance of the CFI-MS method on quantitative analysis of the gaseous sample. Good lineary was obtained (R2 ≥ 0.9946) between 5.0 and 500.0 ng/L with limits of detection (LOD) ranging from 0.5 to 1.5 ng/L. Recoveries of five volatile compounds for the spiked jujube samples were between 94.36 and 106.74% with relative standard deviations (RSDs) less than 7.27% (n = 5). Jujube of different varieties can be distinguished by principal components analysis based on the analytical results of volatile compounds. The developed method demonstrated obvious advantages such as simplicity, high throughput, good sensitivity and wide range of applicability, which will be an alternative way for in situ analysis of the odor sample.
Analysis of risk factor domains in psychosis patient health records
Authors: Holderness Eben , Miller Nicholas , Cawkwell Philip , Bolton Kirsten , Meteer Marie , Pustejovsky James , Hall Mei-Hua
Published: October 2019

Background

Readmission after discharge from a hospital is disruptive and costly, regardless of the reason. However, it can be particularly problematic for psychiatric patients, so predicting which patients may be readmitted is critically important but also very difficult. Clinical narratives in psychiatric electronic health records (EHRs) span a wide range of topics and vocabulary; therefore, a psychiatric readmission prediction model must begin with a robust and interpretable topic extraction component.

Results

We designed and evaluated multiple multilayer perceptron and radial basis function neural networks to predict the sentences in a patient’s EHR that are associated with one or more of seven readmission risk factor domains that we identified. In contrast to our baseline cosine similarity model that is based on the methodologies of prior works, our deep learning approaches achieved considerably better F1 scores (0.83 vs 0.66) while also being more scalable and computationally efficient with large volumes of data. Additionally, we found that integrating clinically relevant multiword expressions during preprocessing improves the accuracy of our models and allows for identifying a wider scope of training data in a semi-supervised setting.

Conclusion

We created a data pipeline for using document vector similarity metrics to perform topic extraction on psychiatric EHR data in service of our long-term goal of creating a readmission risk classifier. We show results for our topic extraction model and identify additional features we will be incorporating in the future.
Effect of bovine lactoferrin as a novel therapeutic agent in a rat model of sepsis-induced acute lung injury
Authors: Han Nannan , Li Hengjie , Li Gang , Shen Ye , Fei Min , Nan Yong
Published: October 2019

Sepsis is a serious clinical condition resulting from severe infection. High rates of mortality and tissue damage have been reported in intensive care unit (ICU) patients with sepsis. Bovine lactoferrin (BLF) is a well-known 80-kDa glycoprotein in the transferrin family that inhibits sepsis in low-birth-weight neonates. The present study investigated the protective effects of BLF in a rat model of sepsis-induced acute lung injury (ALI). The wet/dry ratio, lipid peroxidation, antioxidant markers, total protein, total cell count, inflammatory markers and myeloperoxidase (MPO) levels were assessed. Histopathological analysis was also carried out. BLF treatment reduced the wet/dry ratio of lung tissue by 30.7% and 61.3%, and lipid peroxidation by 22.3% and 67%, at concentrations of 100 and 200 mg/kg, respectively. Superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (Gpx) and catalase were increased by more than 50% under treatment with 200 mg/kg BLF. Inflammatory markers, neutrophils, lymphocytes and total cell count were reduced by more than 50% under treatment with 200 mg/kg BLF. BLF treatment significantly reduced MPO activity, by 28.2% and 74.3%, at concentrations of 100 and 200 mg/kg, respectively. Neutrophilic infiltration and edema were observed in control rats. However, BLF treatment restored intestinal microvilli to the normal range and reduced inflammatory cell invasion. Collectively, these results suggest that BLF is an effective therapeutic agent against sepsis-induced ALI.
The effects of adding angiotensin receptor neprilysin inhibitors to usual care in patients with heart failure: a protocol for a systematic review of randomised clinical trials with meta-analysis and trial sequential analysis
Authors: Nielsen Emil Eik , Feinberg Joshua , Raymond Ilan , Olsen Michael Hecht , Steensgaard-Hansen Frank Victor , Jakobsen Janus Christian
Published: October 2019

Background

Heart failure is a highly prevalent disease with a global prevalence of 37 million, and the prevalence is increasing. Patients with heart failure are at an increased risk of death and morbidity. Traditionally, patients with heart failure have been treated with a beta-blocker in addition to an inhibitor of the renin-angiotensin-aldosterone system. However, new drugs are currently being added to the recommended guideline therapy. The latest drug to be added combines inhibition of the renin-angiotensin-aldosterone system pathway with inhibiting the neprilysin enzyme and is therefore classified as an ARNI. Our objective is to identify the beneficial and harmful effects of ARNIs in the treatment of patient with heart failure.

Methods

This protocol for a systematic review was undertaken using the recommendations of the Cochrane, the Preferred Report Items of Systematic reviews with Meta-Analysis Protocols, and the eight-step assessment procedure suggested by Jakobsen and colleagues. We plan to include all relevant randomised clinical trials assessing the use of ARNIs in the treatment of patients with heart failure. We will search the Cochrane Central Register of Controlled Trials (CENTRAL), Medical Literature Analysis and Retrieval System Online (MEDLINE), Excerpta Medica database (EMBASE), Latin American and Caribbean Health Sciences Literature (LILACS), Science Citation Index Expanded on Web of Science, Chinese Biomedical Literature Database (CBM), China National Knowledge Infrastructure (CNKI), Chinese Science Journal Database (VIP), and BIOSIS to identify relevant trials. We will also search for grey literature and unpublished trials. Extracted data will be analysed using Review Manager 5, STATA 5, and Trial Sequential Analysis. Our primary outcomes will be all-cause mortality and serious adverse events. We will create a ‘Summary of Findings’ table in which we will present our primary and secondary outcomes, and we will assess the quality of evidence using the GRADE assessment.

Discussion

The present systematic review will have the potential to aid clinicians in decision-making and thereby, benefit patients with heart failure.

Systematic review registration

PROSPERO CRD42019129336
Suppressed N fixation and diazotrophs after four decades of fertilization
Authors: Fan Kunkun , Delgado-Baquerizo Manuel , Guo Xisheng , Wang Daozhong , Wu Yanying , Zhu Mo , Yu Wei , Yao Huaiying , Zhu Yong-guan , Chu Haiyan
Published: October 2019

Background

N fixation is one of the most important microbially driven ecosystem processes on Earth, allowing N to enter the soil from the atmosphere, and regulating plant productivity. A question that remains to be answered is whether such a fundamental process would still be that important in an over-fertilized world, as the long-term effects of fertilization on N fixation and associated diazotrophic communities remain to be tested. Here, we used a 35-year fertilization experiment, and investigated the changes in N fixation rates and the diazotrophic community in response to long-term inorganic and organic fertilization.

Results

It was found that N fixation was drastically reduced (dropped by 50%) after almost four decades of fertilization. Our results further indicated that functionality losses were associated with reductions in the relative abundance of keystone and phylogenetically clustered N fixers such as Geobacter spp.

Conclusions

Our work suggests that long-term fertilization might have selected against N fixation and specific groups of N fixers. Our study provides solid evidence that N fixation and certain groups of diazotrophic taxa will be largely suppressed in a more and more fertilized world, with implications for soil biodiversity and ecosystem functions.
An initial performance review of vaginal implant transmitters paired with GPS collars
Authors: Dion Justin R. , Haus Jacob M. , Rogerson Joseph E. , Bowman Jacob L.
Published: October 2019

Background

The vaginal implant transmitter is an effective tool in the study of neonatal survival rates for cervid species. The latest iterations of the vaginal implant transmitter use Global Positioning Systems and ultra-high frequency telemetry technology to create a self-monitoring system in which researchers receive near instantaneous notifications of parturition events via remote transmission. We deployed ultra-high-frequency radio-linked vaginal implant transmitters on 44 adult female white-tailed deer (Odocoileus virginianus) and assessed their performance and possible benefits to field research.

Results

In 2016, 60% of the females expelled transmitters at the birth sites. Failure to locate a birth site was a result of technological failings (20%) and premature expulsions (20%). Following manufacturer updates in 2017, we observed an apparent reduction in technology malfunctions (8%) but similar rates of premature expulsions (33%), which resulted in 58% of radio-linked transmitters expelled at birth sites. We located similar numbers of neonates per device across both years. The likelihood that researchers would locate > 1 neonate at or near the birth site was greater for radio-linked transmitters than has been reported in studies using traditional vaginal implant transmitters.

Conclusions

Radio-linked transmitters allow researchers to increase sample size, expand spatial distribution of study animals, and reduce personnel requirements.
To cross or not to cross – thrushes at the German North Sea coast adapt flight and routing to wind conditions in autumn
Authors: Brust Vera , Michalik Bianca , Hüppop Ommo
Published: October 2019

Background

Although many aspects of passerine migration are genetically determined, routing appears to be flexibly adjusted to the conditions experienced on each individual journey. This holds especially true for routing decisions taken when confronted with large bodies of water. Once taken, these decisions can be hardly altered or revised. In this paper, we analysed stopover and routing decisions taken by three species of thrushes, blackbirds, redwings and song thrushes, at the German North Sea coast.

Methods

Birds were equipped with radio-telemetry tags at stopover sites along the coast during autumn migration and subsequently tracked by an automated receiver network covering the coastline and islands of the German Bight.

Results

The thrushes resumed migration in nights with a favourable northward wind component and clear skies. About 40% of the tagged individuals have taken an offshore instead of an alongshore oriented flight route. Routing decisions were influenced by the strength of the eastward wind component with offshore oriented flights taking place primarily under weak winds or winds blowing towards the west. Thrushes that took an offshore oriented route stopped over at the coast longer than those flying alongshore. Interestingly, offshore as well as alongshore oriented flights co-occurred within single nights and under comparable weather conditions.

Conclusions

Migratory flight and routing decisions of thrushes at the German North Sea coast are highly dependent on weather, in particular wind. Still, we found evidence that weather may not be the sole reason for individual routes taken. Physical condition, morphology or animal personality lend themselves as possible additional factors of influence. Enabling a more detailed understanding of thrush migration over and along the German North Sea, our data help to better judge risks that migratory birds are facing when en route conditions are altered, for example by artificial obstacles such as offshore wind turbines.
A graphical heuristic for reduction and partitioning of large datasets for scalable supervised training
Authors: Yadav Sumedh , Bode Mathis
Published: October 2019

A scalable graphical method is presented for selecting and partitioning datasets for the training phase of a classification task. For the heuristic, a clustering algorithm is required to get its computation cost in a reasonable proportion to the task itself. This step is succeeded by construction of an information graph of the underlying classification patterns using approximate nearest neighbor methods. The presented method consists of two approaches, one for reducing a given training set, and another for partitioning the selected/reduced set. The heuristic targets large datasets, since the primary goal is a significant reduction in training computation run-time without compromising prediction accuracy. Test results show that both approaches significantly speed-up the training task when compared against that of state-of-the-art shrinking heuristics available in LIBSVM. Furthermore, the approaches closely follow or even outperform in prediction accuracy. A network design is also presented for a partitioning based distributed training formulation. Added speed-up in training run-time is observed when compared to that of serial implementation of the approaches.
Comparative effectiveness of Biobrane®, RECELL® Autologous skin Cell suspension and Silver dressings in partial thickness paediatric burns: BRACS randomised trial protocol
Authors: Bairagi Anjana , Griffin Bronwyn , Tyack Zephanie , Vagenas Dimitrios , McPhail Steven M. , Kimble Roy
Published: October 2019

Background

Mixed partial thickness burns are the most common depth of burn injury managed at a large Australian paediatric hospital specialty burns unit. Prolonged time until re-epithelialisation is associated with increased burn depth and scar formation. Whilst current wound management approaches have benefits such as anti-microbial cover, these are not without inherent limitations including multiple dressing changes. The Biobrane® RECELL® Autologous skin Cell suspension and Silver dressings (BRACS) trial aims to identify the most effective wound management approach for mixed partial thickness injuries in children.

Methods

All children presenting with an acute burn injury to the study site will be screened for eligibility. This is a single-centre, three-arm, parallel group, randomised trial. Children younger than 16 years, with burns ≥ 5% total body surface area involving any anatomical location, up to 48 h after the burn injury, and of a superficial partial to mid-dermal depth, will be included. A sample size of 84 participants will be randomised to standard silver dressing or a Regenerative Epithelial Suspension (RES™) with Biobrane® or Biobrane® alone. The first dressing will be applied under general anaesthesia and subsequent dressings will be changed every 3 to 5 days until the wound is ≥ 95% re-epithelialised, with re-epithelialisation time the primary outcome. Secondary outcomes of acute pain, acute itch, scar severity, health-related quality of life, treatment satisfaction, dressing application ease and healthcare resource use will be assessed at each dressing change and 3, 6 and 12 months post-burn injury.

Discussion

The findings of this study can potentially change the wound management approach for superficial partial to mid-dermal burns in children locally and worldwide.

Trial registration

The Australian New Zealand Clinical Trials Registry (ACTRN12618000245291) approved prospective registration on 15 February 2018. Registration details can be viewed at https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=374272&isReview=true.
Using learning analytics to develop early-warning system for at-risk students
Authors: Akçapınar Gökhan , Altun Arif , Aşkar Petek
Published: October 2019

In the current study interaction data of students in an online learning setting was used to research whether the academic performance of students at the end of term could be predicted in the earlier weeks. The study was carried out with 76 second-year university students registered in a Computer Hardware course. The study aimed to answer two principle questions: which algorithms and features best predict the end of term academic performance of students by comparing different classification algorithms and pre-processing techniques and whether or not academic performance can be predicted in the earlier weeks using these features and the selected algorithm. The results of the study indicated that the kNN algorithm accurately predicted unsuccessful students at the end of term with a rate of 89%. When findings were examined regarding the analysis of data obtained in weeks 3, 6, 9, 12, and 14 to predict whether the end-of-term academic performance of students could be predicted in the earlier weeks, it was observed that students who were unsuccessful at the end of term could be predicted with a rate of 74% in as short as 3 weeks’ time. The findings obtained from this study are important for the determination of features for early warning systems that can be developed for online learning systems and as indicators of student success. At the same time, it will aid researchers in the selection of algorithms and pre-processing techniques in the analysis of educational data.
Intra-oropharyngeal food transport and swallowing in white-spotted bamboo sharks
Authors: van Meer Noraly M. M. E. , Weller Hannah I. , Manafzadeh Armita R. , Kaczmarek Elska B. , Scott Bradley , Gussekloo Sander W. S. , Wilga Cheryl D. , Brainerd Elizabeth L. , Camp Ariel L.
Published: October 2019

Despite the importance of intraoral food transport and swallowing, relatively few studies have examined the biomechanics of these behaviors in non-tetrapods, which lack a muscular tongue. Studies show that elasmobranch and teleost fishes generate water currents as a ‘hydrodynamic tongue’ that presumably transports food towards and into the esophagus. However, it remains largely unknown how specific musculoskeletal motions during transport correspond to food motion. Previous studies of white-spotted bamboo sharks (Chiloscyllium plagiosum) hypothesized that motions of the hyoid, branchial arches, and pectoral girdle, generate caudal motion of the food through the long oropharynx of modern sharks. To test these hypotheses, we measured food and cartilage motion with XROMM during intra-oropharyngeal transport and swallowing (n=3 individuals, 2-3 trials per individual). After entering the mouth, food does not move smoothly toward the esophagus, but rather moves in distinct steps with relatively little retrograde motion. Caudal food motion coincides with hyoid elevation and a closed mouth, supporting earlier studies showing that hyoid motion contributes to intra-oropharyngeal food transport by creating caudally-directed water currents. Little correspondence between pectoral girdle and food motion was found, indicating minimal contribution of pectoral girdle motion. Transport speed was fast as food entered the mouth, slower and step-wise through the pharyngeal region and then fast again as it entered the esophagus. The food's static periods in the step-wise motion and its high velocity during swallowing could not be explained by hyoid or girdle motion, suggesting these sharks may also use the branchial arches for intra-oropharyngeal transport and swallowing.
Controlling and measuring dynamic odorant stimuli in the laboratory
Authors: Gorur-Shandilya Srinivas , Martelli Carlotta , Demir Mahmut , Emonet Thierry
Published: October 2019

Animals experience complex odorant stimuli that vary widely in composition, intensity and temporal properties. However, stimuli used to study olfaction in the laboratory are much simpler. This mismatch arises from the challenges in measuring and controlling them precisely and accurately. Even simple pulses can have diverse kinetics that depend on their molecular identity. Here, we introduce a model that describes how stimulus kinetics depend on the molecular identity of the odorant and the geometry of the delivery system. We describe methods to deliver dynamic odorant stimuli of several types, including broadly distributed stimuli that reproduce some of the statistics of naturalistic plumes, in a reproducible and precise manner. Finally, we introduce a method to calibrate a Photo-Ionization Detector to any odorant it can detect, using no additional components. Our approaches are affordable and flexible and can be used to advance our understanding of how olfactory neurons encode real-world odor signals.
Exposure to fluctuating temperatures leads to reduced immunity and to stress response in rattlesnakes
Authors: Fabrício-Neto Ailton , Madelaire Carla B. , Gomes Fernando R. , Andrade Denis V.
Published: October 2019

Ectothermic organisms often experience considerable variation in their body temperature throughout the circadian cycle. However, studies focusing on the measurement of physiological traits are usually performed under constant temperature regimes. This mismatch between thermal exposure in the field and experimental conditions could act as a stressor agent, since physiological functions are strongly influenced by temperature. Herein, we asked the question whether constant thermal regimes would cause a stress response and impact the immunity of the South American rattlesnake (Crotalus durissus) when compared to a fluctuating thermal regime. We addressed this question by determining heterophil:lymphocyte ratio (H:L), plasma bacteria killing ability (BKA) and corticosterone levels (CORT) in snakes kept under a constant temperature regime at 30°C, and under a fluctuating regime that oscillated between 25°C at nighttime to 35°C at daytime. The experiments had a mirrored design, in which half of the snakes were subjected to a fluctuating-to-constant treatment, while the other half was exposed to a constant-to-fluctuating treatment. The shift from constant to fluctuating thermal regime was accompanied by an increase in plasma CORT levels indicating the activation of a stress response. Exposure to a fluctuating thermal regime at the onset of the experiments induced a decrease in the BKA of rattlesnakes. H:L was not affected by treatments and, therefore, the shift between thermal regimes seems to have acted as a low intensity stressor. Our results suggest that the removal from temperatures close to the snakés preferred body temperature triggers a stress response in rattlesnakes.
Slowing down the metabolic engine: impact of early-life corticosterone exposure on adult metabolism in house sparrows (Passer domesticus)
Authors: Dupont Sophie M. , Grace Jacquelyn K. , Lourdais Olivier , Brischoux François , Angelier Frédéric
Published: October 2019

Whole organism metabolism is an integrative process that determines not only the energy cost of living but also the energy output that is available for behavioral and physiological processes during the life cycle. Developmental challenge is known to affect growth, development of several organs, and several physiological mechanisms (such as HPA responsiveness, oxidative stress or immunity), which may altogether affect adult metabolism. All these developmental effects are likely to be mediated by glucocorticoids but the impact of developmental glucocorticoid exposure on adult metabolism has rarely been studied and the results are equivocal. In this study, we examined the impact of developmental exposure to corticosterone (CORT, the main avian glucocorticoid hormone) on RMR (resting metabolic rate, measured in thermoneutrality, 25°C) and TMR (thermoregulatory metabolic rate, measured in cold challenging condition, 5°C) in the House sparrow. Following experimental administration of CORT at the nestling stage, house sparrows were kept in captivity until adulthood when their metabolism was measured. We found that post-natal CORT exposure decreased both RMR and TMR in adult sparrows. This CORT-mediated reduction of metabolism was also associated with a reduced overnight body mass loss. Therefore, our results suggest that developmental CORT exposure can orient the phenotype towards an energy-saving strategy, which may be beneficial in a constraining environmental context.
Indicator for local recurrence of hepatocellular carcinoma after proton beam therapy: analysis of attenuation difference between the irradiated tumor and liver parenchyma on contrast enhancement CT
Authors: Takahashi Hiroaki , Sekino Yuta , Mori Kensaku , Okumura Toshiyuki , Nasu Katsuhiro , Fukuda Kuniaki , Masuoka Sota , Iizumi Takashi , Hasegawa Naoyuki , Sakurai Hideyuki , Minami Manabu
Published: October 2019

Objectives:

We aimed to identify dynamic CT features that can be used for prediction of local recurrence of hepatocellular carcinoma (HCC) after proton beam therapy (PBT).

Methods:

We retrospectively retrieved CT scans of patients with PBT-treated HCC, taken between January 2004 and December 2016. 17 recurrent lesions and 34 non-recurrent lesions were retrieved. The attenuation difference between irradiated tumor and irradiated parenchyma (ADHCC-IP) was compared in the two groups by using the Mann–Whitney U test. Cut-off value of ADHCC-IP was estimated by using the Youden index.

Results:

The follow-up time after PBT initiation ranged from 374 to 2402 days (median, 1069 days) in recurrent lesions, and 418 to 2923 days (median, 1091.5 days) in non-recurrent lesions (p = 0.892). The time until appearance of local recurrence after PBT initiation ranged from 189 to 2270 days (median, 497 days). ADHCC-IP of recurrent lesions [mean, −21.8 Hounsfield units (HU); from −95 to −31 HU] was significantly greater than that of non-recurrent lesions (mean, −51.7 HU; from −117 to −12 HU) at 1–2 years in portal venous phase (p = 0.039). 5-year local tumor control rates were 0.93 and 0.56 in lesions with ADHCC-IP at 1–2 years in PVP < −55 and ≥ −55 HU, respectively.

Conclusion:

The attenuation difference between irradiated HCC and irradiated liver parenchyma in portal venous phase at 1-2 years after PBT can predict long-term local recurrence of HCC after treatment.

Advances in knowledge:

We identified a cut-off value for contrast enhancement of HCC after PBT that could predict future local recurrence.
Small masses (≤3 cm) diagnosed as hepatocellular carcinoma on pre-treatment imaging: comparison of therapeutic outcomes between hepatic resection and radiofrequency ablation
Authors: Cha Dong Ik , Song Kyoung Doo , Kang Tae Wook , Lee Min Woo , Rhim Hyunchul
Published: October 2019

Objective:

To compare therapeutic outcomes between hepatic resection (HR) and radiofrequency ablation (RFA) for small hepatic masses diagnosed as hepatocellular carcinoma (HCC) on pre-treatment imaging study.

Methods:

Our institutional review board approved this retrospective study, and informed consent was waived. Patients with a single (≤3 cm) mass diagnosed as HCC on pre-treatment imaging study between January 2008 and December 2009 who underwent HR (n = 145) or RFA (n = 178) were included. Recurrence-free survival (RFS) and overall survival (OS) were assessed. In the HR group, the false-positive rate for imaging diagnosis was calculated. For the RFA group, the local tumor progression rate was calculated.

Results:

RFS rates at 5 years were 59.3% for the HR group and 32.2% for the RFA group. OS rates at 5 years were 85.4% for the HR group and 76.8% for the RFA group. In the RFA group, cumulative local tumor progression rates were 8.3 and 20.2% at 1 and 3 years. Treatment modality was not an independent prognostic factor for either RFS or OS on multivariate analysis. The false-positive rate for HCC diagnosis based on imaging criteria was 4.8% in the HR group.

Conclusion:

The imaging criteria for diagnosis of HCC have a high positive predictive value. Multivariate analysis showed that RFS and OS rates were not significantly different between HR and RFA for small hepatic masses diagnosed as HCC on pre-treatment imaging.

Advances in knowledge:

Treatment modality (hepatic resection vs RFA) was not an independent prognostic factor for both RFS and OS for small masses (≤3 cm) diagnosed as hepatocellular carcinoma on pre-treatment imaging.
An update on effects of ionizing radiation exposure on the eye
Authors: Hamada Nobuyuki , Azizova Tamara V. , Little Mark P.
Published: October 2019

The International Commission on Radiological Protection (ICRP) has considered for over 60 years that the lens of the eye is among the most radiosensitive tissues, and has recommended dose limits for the lens to prevent occurrence of vision impairing cataracts (VICs). Epidemiological evidence that doses much lower than previously thought produce cataracts led ICRP to recommend reducing dose threshold for VICs and reducing an occupational equivalent dose limit for the lens in 2011, when only a single threshold of 0.5 Gy was recommended. On the basis of epidemiological evidence, ICRP assumed progression of minor opacities into VICs and no dose rate effect. This contrasts with previously recommended separate thresholds for minor opacities and VICs, and for different exposure scenarios. Progression was assumed based on similar risks of cataracts and cataract surgery in Japanese atomic bomb survivors. The absence of dose rate effect derived from the observed similar thresholds for protracted exposures in Chernobyl cleanup workers and in atomic bomb survivors. Since 2011, there has been an increasing body of epidemiological evidence relating to cataracts and other ocular diseases (i.e. glaucoma and macular degeneration), particularly at low doses and low dose rates. This review paper gives an overview of the scientific basis of the 2011 ICRP recommendation, discusses the plausibility of these two assumptions in the light of emerging scientific evidence, and considers the radiosensitivity of the lens among ocular structures.
CT-like MRI using the zero-TE technique for osseous changes of the TMJ
Authors: Lee Chena , Jeon Kug Jin , Han Sang-Sun , Kim Young Hyun , Choi Yoon Joo , Lee Ari , Choi Jong Hoon
Published: October 2019

Objective:

This study was conducted to assess the clinical usability of the zero-echo time (ZTE) technique of MRI for evaluating bone changes of the temporomandibular joint (TMJ) in comparison with CBCT.

Methods:

Twenty patients with TMJ disorder who underwent both CBCT and MRI were randomly selected. CBCT images were obtained with an Alphard 3030 device (Asahi Roentgen Ind., Co. Ltd, Kyoto, Japan). MRIs were obtained using a 3.0 T scanner (Pioneer; GE Healthcare, Waukesha, WI, USA) and a 21-channel head coil. An isotropic three-dimensional proton-density-weighted ZTE sequence was acquired. Two radiologists evaluated 40 joints of 20 patients for the presence of the following osseous changes: flattening, erosion, osteophyte and sclerosis of the condyle; and flattening, erosion and sclerosis of the articular fossa. CBCT and ZTE-MRI assessments were performed at a 2-month interval. The prevalence-adjusted and bias-adjusted κ statistic was used to analyse interexaminer and intraexaminer agreement and the agreement between ZTE-MRI and CBCT.

Results:

Intraexaminer and interexaminer agreement analyses of ZTE-MRI showed high reproducibility (κ>0.80), which was comparable to that of CBCT. Flattening, osteophyte and sclerosis of the condyle and all types of bone changes in the mandibular fossa showed nearly perfect agreement between CBCT and ZTE-MRI (κ = 0.80–0.90). Erosion of the condyle showed substantial agreement between both sets of images (κ = 0.65–0.70).

Conclusions:

It is suggested that ZTE-MRI provides clinically reliable images for bone assessment in TMJ disorder. MRI may become a beneficial diagnostic tool for patients with both TMJ disc and bone pathology, with advantages involving medical costs and radiation dose.
Past, present, and future efforts to enhance the efficacy of cord blood hematopoietic cell transplantation
Authors: Huang Xinxin , Guo Bin , Capitano Maegan , Broxmeyer Hal E.
Published: October 2019

Cord blood (CB) has been used as a viable source of hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) in over 35,000 clinical hematopoietic cell transplantation (HCT) efforts to treat the same variety of malignant and non-malignant disorders treated by bone marrow (BM) and mobilized peripheral blood (mPB) using HLA-matched or partially HLA-disparate related or unrelated donor cells for adult and children recipients. This review documents the beginning of this clinical effort that started in the 1980’s, the pros and cons of CB HCT compared to BM and mPB HCT, and recent experimental and clinical efforts to enhance the efficacy of CB HCT. These efforts include means for increasing HSC numbers in single CB collections, expanding functional HSCsex vivo, and improving CB HSC homing and engraftment, all with the goal of clinical translation. Concluding remarks highlight the need for phase I/II clinical trials to test the experimental procedures that are described, either alone or in combination.
Chemokine regulation of inflammation during respiratory syncytial virus infection
Authors: Nuriev Rinat , Johansson Cecilia
Published: October 2019

Respiratory syncytial virus (RSV) can cause severe lower respiratory tract infections especially in infants, immunocompromised individuals and the elderly and is the most common cause of infant hospitalisation in the developed world. The immune responses against RSV are crucial for viral control and clearance but, if dysregulated, can also result in immunopathology and impaired gas exchange. Lung immunity to RSV and other respiratory viruses begins with the recruitment of immune cells from the bloodstream into the lungs. This inflammatory process is controlled largely by chemokines, which are small proteins that are produced in response to innate immune detection of the virus or the infection process. These chemokines serve as chemoattractants for granulocytes, monocytes, lymphocytes and other leukocytes. In this review, we highlight recent advances in the field of RSV infection and disease, focusing on how chemokines regulate virus-induced inflammation.
Profile Comparer Extended: phylogeny of lytic polysaccharide monooxygenase families using profile hidden Markov model alignments
Authors: Voshol Gerben P. , Punt Peter J. , Vijgenboom Erik
Published: October 2019

Insight into the inter- and intra-family relationship of protein families is important, since it can aid understanding of substrate specificity evolution and assign putative functions to proteins with unknown function. To study both these inter- and intra-family relationships, the ability to build phylogenetic trees using the most sensitive sequence similarity search methods (e.g. profile hidden Markov model (pHMM)–pHMM alignments) is required. However, existing solutions require a very long calculation time to obtain the phylogenetic tree. Therefore, a faster protocol is required to make this approach efficient for research. To contribute to this goal, we extended the original Profile Comparer program (PRC) for the construction of large pHMM phylogenetic trees at speeds several orders of magnitude faster compared to pHMM-tree. As an example, PRC Extended (PRCx) was used to study the phylogeny of over 10,000 sequences of lytic polysaccharide monooxygenase (LPMO) from over seven families. Using the newly developed program we were able to reveal previously unknown homologs of LPMOs, namely the PFAM Egh16-like family. Moreover, we show that the substrate specificities have evolved independently several times within the LPMO superfamily. Furthermore, the LPMO phylogenetic tree, does not seem to follow taxonomy-based classification.
history of Russia. Challenges of the Romanov era
Authors: Жеребкин Михаил , Zherebkin Mihail
Published: October 2019

Textbook " History of Russia. Challenges of the Romanov era "is a continuation of the author's research" History of Russia. Challenges of the Rurik era". The book analyzes the most important events and actors of Russian history through the prism of historical conflicts during the three centuries of the reign of the Romanov dynasty tsars: from Mikhail Fedorovich to Nicholas II.
Meets the requirements of the Federal state educational standards of higher education of the last generation in the direction of 46.03.01 "History".
It is recommended to students and teachers of historical faculties of universities, teachers of history of schools and all who are interested in history of Russia.