bluuebunny/crossref_metadata_2025 huggingface dataset:
Environmental Quality Perceptions and Health: A Cross-Sectional Study of Citizens of Kaunas, Lithuania
Authors: Gražulevičienė Regina , Andrušaitytė Sandra , Dėdelė Audrius , Gražulevičius Tomas , Valius Leonas , Kapustinskienė Violeta , Bendokienė Inga
Published: June 2020

The perception of urban environmental quality is an important contributor when identifying local problems in sustainable development and environmental planning policy. This study examined the associations between environmental and social residential characteristics, physical activity, obesity, and hypertension in Kaunas city, Lithuania. This cross-sectional study analyzed 580 citizens’ demographic-, socioeconomic-, health-, and lifestyle-related factors, environmental health concerns, and environmental quality perceptions. Using Geographic Information Systems and the multivariate logistic regression, we found that the less physically active group more often presented lower than mean ratings of the quality of pathways and cycling routes (32.9% and 45.6%, p = 0.042) and only irregularly visited the natural environment. Obese participants presented poorer ratings of air pollution, the quality of pathways and cycling routes, their possibility to reach green spaces by walking, and the available relaxing areas. The environmental issues associated with hypertension were poor possibilities to reach green spaces by walking (OR 1.94, 95% CI 1.14–3.32) and the availability of relaxation areas (OR 2.30, 95% 1.34–3.95). The quality of the neighborhood and individual-level characteristics were the factors that influenced a higher prevalence of health problems at the district level. Our findings suggest that a public health policy to improve the physical and social environment of the neighborhood would have a potential to increase citizens’ physical activity and health.
“Study Natural” without Drugs: An Exploratory Study of Theory-Guided and Tailored Health Campaign Interventions to Prevent Nonmedical Use of Prescription Stimulants in College Students
Authors: Su Zhaohui , Mackert Michael , Li Xiaoshan , Han Jiyoon (Karen) , Crook Brittani , Wyeth Benjamin
Published: June 2020

Nonmedical use of prescription stimulants (NMUPS) among college students continues to rise. While some anti-NMUPS campaigns are available, little is known about the campaign development process and how well college students evaluate these messages. To bridge this gap, we developed theory-guided anti-NMUPS campaign interventions that are tailored to college students’ characteristics and evaluated students’ response towards them. A total of 445 college students (74.4% female; Meanage of 20; 18 to 35 years old) reviewed the campaign interventions and offered their evaluation via an online survey. Findings indicate that students responded to the campaigns positively. Results also indicate that female students are more likely to perceive the campaigns as effective than their male counterparts. Overall, the findings of this study suggest that theory-guided and tailored anti-NMUPS campaigns have great potential in changing students’ attitudes and behavior towards NMUPS. While this study fills critical gaps in the literature, considering the progress needed to strengthen the research field, more research is needed to further identify effective strategies that could prevent college students’ participation in NMUPS activities.
Understanding the Role of Social Interactions in the Development of an Extracurricular University Volunteer Activity in a Developing Country
Authors: Díaz-Iso Ariane , Eizaguirre Almudena , García-Olalla Ana
Published: June 2020

The relevance of participating in structured extracurricular activities (ECA from here onwords) in higher education is increasing. Involvement in these activities helps students develop personal and social skills that positively affect academic and professional training, well-being, and the development of attitudes toward community involvement. This study analyzes the role of social interactions in the perceived benefits of students, who have participated in an ECA in a developing country. Moreover, this research aspires to explore whether students perceive that these interactions positively impact academic training, professional development, and adjustment to the university context, psychological well-being, and development of community involvement attitudes. As a result, 46 in-depth interviews were conducted with 23 students who participated in the experience. 23 in-depth interviews were conducted before the experience and another 23 after it. Data analysis was carried out using the IRaMuTeQ software to conduct a descending hierarchical classification (DHC). This study highlights the value of social interactions in an ECA to increase the motivation of students to improve academic and professional performance, to build shared knowledge with others, and to develop personal and social skills that contribute to the integral development of participants. ECAs help students reflect on their actions and privileges and develop positive attitudes toward themselves and others. This fact is linked to the achievement of high levels of well-being that allow the enhancement of the students’ self-esteem. Finally, this experience has allowed volunteers to become aware of other sociocultural realities and to reflect on the possible ways of contributing to the development of a more sustainable society.
Current Applications, Opportunities, and Limitations of AI for 3D Imaging in Dental Research and Practice
Authors: Hung Kuofeng , Yeung Andy Wai Kan , Tanaka Ray , Bornstein Michael M.
Published: June 2020

The increasing use of three-dimensional (3D) imaging techniques in dental medicine has boosted the development and use of artificial intelligence (AI) systems for various clinical problems. Cone beam computed tomography (CBCT) and intraoral/facial scans are potential sources of image data to develop 3D image-based AI systems for automated diagnosis, treatment planning, and prediction of treatment outcome. This review focuses on current developments and performance of AI for 3D imaging in dentomaxillofacial radiology (DMFR) as well as intraoral and facial scanning. In DMFR, machine learning-based algorithms proposed in the literature focus on three main applications, including automated diagnosis of dental and maxillofacial diseases, localization of anatomical landmarks for orthodontic and orthognathic treatment planning, and general improvement of image quality. Automatic recognition of teeth and diagnosis of facial deformations using AI systems based on intraoral and facial scanning will very likely be a field of increased interest in the future. The review is aimed at providing dental practitioners and interested colleagues in healthcare with a comprehensive understanding of the current trend of AI developments in the field of 3D imaging in dental medicine.
Distribution of Geochemical Fractions of Phosphorus in Surface Sediment in Daya Bay, China
Authors: Liao Hongping , Pan Ciguang , Gan Lian , Ke Zhixin , Tang Huijuan
Published: June 2020

Surface sediment samples were collected from 19 sites throughout Daya Bay, China, to study the concentrations, and spatial distributions of different fractions of phosphorus through sequential extraction methods. Like many coastal and marine areas, De-P was the dominant form of P, contributing 47.5% of TP, followed by O-P, contributing 25.5% of TP. Ex-P and Fe-P contribute the lowest to TP. The concentration of sedimentary TP ranged from 290.3~525.1 µg/g, with the average of 395.3 µg/g, which was a similar range to other estuaries and coastal areas. Based on the spatial distribution, Pearson correlation and Principal component analysis, different fractions of phosphorus showed different spatial distributions due to different sources. The molar ratio of organic carbon to phosphorus (TOC/O-P) ranged from 199 to 609, with the average of 413, which was much higher than the Redfield ratio, suggesting terrestrial sources of organic matter in Daya Bay surface sediment. The average bioavailable phosphorus was 149.6 µg/g and contributed 37.8% (24.6~56.0%) of TP, indicating that the surface sediments of Day Bay act as an important internal source of P.
Outcomes of the Health Insurance Card Scheme on Migrants’ Use of Health Services in Ranong Province, Thailand
Authors: Phaiyarom Mathudara , Pudpong Nareerut , Suphanchaimat Rapeepong , Kunpeuk Watinee , Julchoo Sataporn , Sinam Pigunkaew
Published: June 2020

In 2002, Thailand achieved Universal Health Coverage for all citizens; however, it remains the case that undocumented migrants are not fully covered. The Health Insurance Card Scheme (HICS) of the Ministry of Public Health is the key policy aiming to cover undocumented migrants. This study examined the impact of this policy on the utilisation rate of public health facilities among HICS beneficiaries including undocumented migrants. Facility-based individual records between 2011 and 2015 were purposively retrieved from one provincial hospital, one district hospital, and two health centres in one of the most densely migrant-populated provinces in Thailand. Poisson regression was conducted on inpatient (IP) utilisation, while negative binomial regression was conducted on outpatient (OP) utilisation. Of 74,722 admissions, 19.0% were insured by HICS. About 14.0% of the outpatient records were for HICS beneficiaries. Overall, the HICS utilisation rate in migrants was lower than in Thai patients. Being insured with the HICS significantly increased OP utilisation by 1.7%, and IP utilisation by 11.1% (relative to uninsured). Disease status was the most important factor that positively influenced the utilisation rate. Further studies that explore the differences in health service utilisation among HICS beneficiaries with diverse economic backgrounds are recommended
NKG2A/CD94 Is a New Immune Receptor for HLA-G and Distinguishes Amino Acid Differences in the HLA-G Heavy Chain
Authors: Hò Gia-Gia T. , Celik Alexander A. , Huyton Trevor , Hiemisch Wiebke , Blasczyk Rainer , Simper Gwendolin S. , Bade-Doeding Christina
Published: June 2020

Natural killer (NK) cell therapies are a tool to antagonize a dysfunctional immune system. NK cells recognize malignant cells, traffic to a tumor location, and infiltrate the solid tumor. The immune checkpoint molecule human leukocyte antigen (HLA)-G is upregulated on malignant cells but not on healthy surrounding cells, the requirement of understanding the basis of receptor mediated events at the HLA-G/NK cell interface becomes obvious. The NK cell receptors ILT2 and KIR2DL4 have been described to bind to HLA-G; however, their differential function and expression levels on NK cell subsets suggest the existence of an unreported receptor. Here, we performed a ligand-based receptor capture on living cells utilizing sHLA-G*01:01 molecules coupled to TriCEPS and bound to NK cells followed by mass spectrometric analyses. We could define NKG2A/CD94 as a cognate receptor of HLA-G. To verify the results, we used the reciprocal method by expressing recombinant soluble heterodimeric NKG2A/CD94 molecules and used them to target HLA-G*01:01 expressing cells. NKG2A/CD94 could be confirmed as an immune receptor of HLA-G*01:01. Despite HLA-G is marginal polymorphic, we could previously demonstrate that the most common allelic subtypes HLA-G*01:01/01:03 and 01:04 differ in peptide repertoire, their engagement to NK cells, their catalyzation of dNK cell proliferation and their impact on NK cell development. Continuing these studies with regard to NKG2A/CD94 engagement we engineered recombinant single antigen presenting K562 cells and targeted the surface expressed HLA-G*01:01, 01:03 or 01:04 molecules with NKG2A/CD94. Specificity and sensitivity of HLA-G*01:04/NKG2A/CD94 engagement could be significantly verified. The binding affinity decreases when using K562-G*01:03 or K562-G*01:01 cells as targets. These results demonstrate that the ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
MicroRNA and ROS Crosstalk in Cardiac and Pulmonary Diseases
Authors: Climent Montserrat , Viggiani Giacomo , Chen Ya-Wen , Coulis Gerald , Castaldi Alessandra
Published: June 2020

Reactive oxygen species (ROS) affect many cellular functions and the proper redox balance between ROS and antioxidants contributes substantially to the physiological welfare of the cell. During pathological conditions, an altered redox equilibrium leads to increased production of ROS that in turn may cause oxidative damage. MicroRNAs (miRNAs) regulate gene expression at the post-transcriptional level contributing to all major cellular processes, including oxidative stress and cell death. Several miRNAs are expressed in response to ROS to mediate oxidative stress. Conversely, oxidative stress may lead to the upregulation of miRNAs that control mechanisms to buffer the damage induced by ROS. This review focuses on the complex crosstalk between miRNAs and ROS in diseases of the cardiac (i.e., cardiac hypertrophy, heart failure, myocardial infarction, ischemia/reperfusion injury, diabetic cardiomyopathy) and pulmonary (i.e., idiopathic pulmonary fibrosis, acute lung injury/acute respiratory distress syndrome, asthma, chronic obstructive pulmonary disease, lung cancer) compartments. Of note, miR-34a, miR-144, miR-421, miR-129, miR-181c, miR-16, miR-31, miR-155, miR-21, and miR-1/206 were found to play a role during oxidative stress in both heart and lung pathologies. This review comprehensively summarizes current knowledge in the field.
ApoE4 Astrocytes Secrete Basement Membranes Rich in Fibronectin and Poor in Laminin Compared to ApoE3 Astrocytes
Authors: Keable Abby , O’Neill Ronan , MacGregor Sharp Matthew , Gatherer Maureen , Yuen Ho Ming , Johnston David Annandale , Weller Roy Oliver , Carare Roxana Octavia
Published: June 2020

The accumulation of amyloid-β (Aβ) in the walls of capillaries and arteries as cerebral amyloid angiopathy (CAA) is part of the small vessel disease spectrum, related to a failure of elimination of Aβ from the brain. Aβ is eliminated along basement membranes in walls of cerebral capillaries and arteries (Intramural Peri-Arterial Drainage—IPAD), a pathway that fails with age and ApolipoproteinEε4 (ApoE4) genotype. IPAD is along basement membranes formed by capillary endothelial cells and surrounding astrocytes. Here, we examine (1) the composition of basement membranes synthesised by ApoE4 astrocytes; (2) structural differences between ApoE4 and ApoE3 astrocytes, and (3) how flow of Aβ affects Apo3/4 astrocytes. Using cultured astrocytes expressing ApoE3 or ApoE4, immunofluorescence, confocal, correlative light and electron microscopy (CLEM), and a millifluidic flow system, we show that ApoE4 astrocytes synthesise more fibronectin, possess smaller processes, and become rarefied when Aβ flows over them, as compared to ApoE3 astrocytes. Our results suggest that basement membranes synthesised by ApoE4 astrocytes favour the aggregation of Aβ, its reduced clearance via IPAD, thus promoting cerebral amyloid angiopathy.
Kallikrein-Related Peptidase 14 Activates Zymogens of Membrane Type Matrix Metalloproteinases (MT-MMPs)—A CleavEx Based Analysis
Authors: Falkowski Katherine , Bielecka Ewa , Thøgersen Ida B. , Bocheńska Oliwia , Płaza Karolina , Kalińska Magdalena , Sąsiadek Laura , Magoch Małgorzata , Pęcak Aleksandra , Wiśniewska Magdalena , Gruba Natalia , Wysocka Magdalena , Wojtysiak Anna , Brzezińska-Bodal Magdalena , Sychowska Kamila , Pejkovska Anastasija , Rehders Maren , Butler Georgina , Overall Christopher M , Brix Klaudia , Dubin Grzegorz , Lesner Adam , Kozik Andrzej , Enghild Jan J. , Potempa Jan , Kantyka Tomasz
Published: June 2020

Kallikrein-related peptidases (KLKs) and matrix metalloproteinases (MMPs) are secretory proteinases known to proteolytically process components of the extracellular matrix, modulating the pericellular environment in physiology and in pathologies. The interconnection between these families remains elusive. To assess the cross-activation of these families, we developed a peptide, fusion protein-based exposition system (Cleavage of exposed amino acid sequences, CleavEx) aiming at investigating the potential of KLK14 to recognize and hydrolyze proMMP sequences. Initial assessment identified ten MMP activation domain sequences which were validated by Edman degradation. The analysis revealed that membrane-type MMPs (MT-MMPs) are targeted by KLK14 for activation. Correspondingly, proMMP14-17 were investigated in vitro and found to be effectively processed by KLK14. Again, the expected neo-N-termini of the activated MT-MMPs was confirmed by Edman degradation. The effectiveness of proMMP activation was analyzed by gelatin zymography, confirming the release of fully active, mature MT-MMPs upon KLK14 treatment. Lastly, MMP14 was shown to be processed on the cell surface by KLK14 using murine fibroblasts overexpressing human MMP14. Herein, we propose KLK14-mediated selective activation of cell-membrane located MT-MMPs as an additional layer of their regulation. As both, KLKs and MT-MMPs, are implicated in cancer, their cross-activation may constitute an important factor in tumor progression and metastasis.
Validation of Enhancing Effects of Curcumin on Radiotherapy with F98/FGT Glioblastoma-Bearing Rat Model
Authors: Wang Wei-Hsun , Shen Chao-Yu , Chien Yi-Chun , Chang Wen-Shin , Tsai Chia-Wen , Lin Yi-Hsien , Hwang Jeng-Jong
Published: June 2020

Glioblastoma, the most common and aggressive brain tumor with low survival rate, is difficult to be cured by neurosurgery or radiotherapy. Mounting evidence has reported the anti-inflammatory and anticancer effects of curcumin on several types of cancer in preclinical studies and clinical trials. To our knowledge, there is no platform or system that could be used to effectively and real-timely evaluate the therapeutic efficacy of curcumin for glioblastoma multiforme (GBM). In this study, we constructed a lentivirus vector with triple-reporter genes (Fluc/GFP/tk) and transduced into rat F98 glioblastoma cells to establish an orthotopic F98/FGT glioma-bearing rat model. In the model, the therapeutic efficacies for curcumin alone, radiation alone, and their combination were evaluated via noninvasive bioluminescent imaging and overall survival measurements. At the cell level, curcumin is capable of causing a G2/M cell cycle arrest and sensitizing the F98 cells to radiation. In animal model, curcumin synergistically enhances the effects of radiotherapy on suppressing the growth of both transplanted glioma cells and in situ brain tumors, and extending the overall survival periods longer than those of curcumin alone and radiation alone treatments. In conclusion, we have demonstrated that curcumin may serve as a novel radiosensitizer to combine with radiotherapy using the triple-reporter F98/FGT animal model for effective and simultaneous evaluation of therapeutic efficacy.
Proteome Analysis Reveals Syndecan 1 Regulates Porcine Sapelovirus Replication
Authors: Zhao Tingting , Cui Li , Yu Xiangqian , Zhang Zhonghai , Chen Qi , Hua Xiuguo
Published: June 2020

Porcine sapelovirus A (PSV) is a single stranded, positive-sense, non-enveloped RNA virus that causes enteritis, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs. Research on PSV infection and interaction with host cells is unclear. In this study, we applied tandem mass tag proteomics analysis to investigate the differentially expressed proteins (DEPs) in PSV-infected pig kidney (PK)-15 cells and explored the interactions between PSV and host cells. Here we mapped 181 DEPs, including 59 up-regulated and 122 down-regulated DEPs. Among them, osteopontin (SPP1), induced protein with tetratricopeptide repeats 5 (IFIT5), ISG15 ubiquitin-like modifier (ISG15), vinculin (VCL), and syndecan-1 (SDC1) were verified significantly changed using RT-qPCR. Additionally, overexpression of SDC1 promoted PSV viral protein (VP)1 synthesis and virus titer, and silencing of SDC1 revealed the opposite results. Our findings show that SDC1 is a novel host protein and plays crucial roles in regulating PSV replication.
The Influence of a Terminal Chlorine Substituent on the Kinetics and the Mechanism of the Solvolyses of n-Alkyl Chloroformates in Hydroxylic Solvents
Authors: D’Souza Malcolm J. , Wirick Jeremy , Mahmoud Osama , Kevill Dennis N. , Kyong Jin Burm
Published: June 2020

A previous study of the effect of a 2-chloro substituent on the rates and the mechanisms of the solvolysis of ethyl chloroformate is extended to the effect of a 3-chloro substituent on the previously studied solvolysis of propyl chloroformate and to the effect of a 4-chloro substituent on the here reported rates of solvolysis of butyl chloroformate. In each comparison, the influence of the chloro substituent is shown to be nicely consistent with the proposal, largely based on the application of the extended Grunwald–Winstein equation, of an addition-elimination mechanism for solvolysis in the solvents of only modest solvent ionizing power, which changes over to an ionization mechanism for solvents of relatively high ionizing power and low nucleophilicity, such as aqueous fluoroalcohols with an appreciable fluoroalcohol content.
Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People
Authors: Kushkevych Ivan , Castro Sangrador Jorge , Dordević Dani , Rozehnalová Monika , Černý Martin , Fafula Roman , Vítězová Monika , Rittmann Simon K.-M. R.
Published: June 2020

Background: Inflammatory bowel diseases (IBDs) are multifactorial illnesses of the intestine, to which microorganisms are contributing. Among the contributing microorganisms, sulfate-reducing bacteria (SRB) are suggested to be involved in the process of bowel inflammation due to the production of hydrogen sulfide (H2S) by dissimilatory sulfate reduction. The aims of our research were to physiologically examine SRB in fecal samples of patients with IBD and a control group, their identification, the study of the process of dissimilatory sulfate reduction (sulfate consumption and H2S production) and biomass accumulation. Determination of biogenic elements of the SRB and evaluation of obtained parameters by using statistical methods were also included in the research. The material for the research consisted of 14 fecal samples, which was obtained from patients and control subjects. Methods: Microscopic techniques, microbiological, biochemical, biophysical methods and statistical analysis were included. Results: Colonies of SRB were isolated from all the fecal samples, and subsequently, 35 strains were obtained. Vibrio-shaped cells stained Gram-negative were dominant in all purified studied strains. All strains had a high percentage of similarity by the 16S rRNA gene with deposited sequences in GenBank of Desulfovibrio vulgaris. Cluster analysis of sulfate reduction parameters allowed the grouping of SRB strains. Significant (p < 0.05) differences were not observed between healthy individuals and patients with IBD with regard to sulfate reduction parameters (sulfate consumption, H2S and biomass accumulation). Moreover, we found that manganese and iron contents in the cell extracts are higher among healthy individuals in comparison to unhealthy individuals that have an intestinal bowel disease, especially ulcerative colitis. Conclusions: The observations obtained from studying SRB emphasize differences in the intestinal microbial processes of healthy and unhealthy people.
Alterations in Adiponectin, Leptin, Resistin, Testosterone, and Cortisol across Eleven Weeks of Training among Division One Collegiate Throwers: A Preliminary Study
Authors: Hornsby W. Guy , Haff G. Gregory , Suarez Dylan G. , Ramsey Michael W. , Triplett N. Travis , Hardee Justin P. , Stone Margaret E. , Stone Michael H.
Published: June 2020

Cytokine and hormone concentrations can be linked to the manipulation of training variables and to subsequent alterations in performance. Subjects: Nine D-1 collegiate throwers and 4 control subjects participated in this preliminary and exploratory report. Methods: Hormone (testosterone (T) and cortisol (C)) and adipokine (adiponectin, leptin, and resistin) measurements were taken at weeks 1, 7, and 11 for the throwers and weeks 1 and 11 for the control group. The throwers participated in an 11-week periodized resistance training and throws program during the fall preparatory period. Volume load was recorded throughout the study. Results: Hormone values did not exhibit statistically significant changes across time; however, there were notable changes for C, the testosterone to cortisol ratio (T:C), and adiponectin. Conclusions: T:C was increased as volume load decreased, and adiponectin increased in concert with decreases in C and increases in the T:C, possibly suggesting a lesser degree of obesity-related inflammation and a higher degree of “fitness” and preparedness.
Classification Models for Skin Tumor Detection Using Texture Analysis in Medical Images
Authors: Almeida Marcos A. M. , Santos Iury A. X.
Published: June 2020

Medical images have made a great contribution to early diagnosis. In this study, a new strategy is presented for analyzing medical images of skin with melanoma and nevus to model, classify and identify lesions on the skin. Machine learning applied to the data generated by first and second order statistics features, Gray Level Co-occurrence Matrix (GLCM), keypoints and color channel information—Red, Green, Blue and grayscale images of the skin were used to characterize decisive information for the classification of the images. This work proposes a strategy for the analysis of skin images, aiming to choose the best mathematical classifier model, for the identification of melanoma, with the objective of assisting the dermatologist in the identification of melanomas, especially towards an early diagnosis.
Wave Orbital Velocity Effects on Radar Doppler Altimeter for Sea Monitoring
Authors: Reale Ferdinando , Pugliese Carratelli Eugenio , Di Leo Angela , Dentale Fabio
Published: June 2020

The orbital velocity of sea wave particles affects the value of sea surface parameters as measured by radar Doppler altimeters (also known as delay Doppler altimeter (DDA)). In DDA systems, the along-track resolution is attained by algorithms that take into account the Doppler shift induced by the component along the Earth/antenna direction of the satellite velocity, VS. Since the vertical component of the wave particle orbital velocity also induces an additional Doppler effect (in the following R-effect), an error arises on the positioning of the target on the sea surface. A numerical investigation shows that when the wavelength of sea waves is of the same order of magnitude of the altimeter resolution, the shape of the waveform might be significantly influenced by the R-effect. The phenomenon can be particularly important for the monitoring of long swells, such as those that often take place in the oceans.
A Study on Barge-Bank Interaction Forces Considering the Reflected Waves
Authors: Hong Chunbeom , Lee Sangmin
Published: June 2020

Because of the relative location between the ship and the bank, the fluid flow becomes complicated such that unstable hydrodynamic forces result in the yaw movement of the ship in an unintended direction. To consider the nonlinear effect, this study calculated the lateral force and yaw moment of barges with different shapes in confined waters, using computational fluid dynamics (CFD). We analyzed the effect of the reflected waves from the bank on the barges. The sway force tended to increase as both barges became closer to the bank, because it worked as a suction force that pulled them toward the closest bank. The yaw moment increased as the barges became closer to the bank, regardless of the shape of the bow. At y′ = 0.2B, when the barges were at the closest to the bank, it rapidly soared. The wave pattern showed that the diverging waves from the shoulder did not disperse, and were blocked by the bank and returned to the ship; such phenomena resulted in changing the hydrodynamic force on the barge. It is determined that the effect of free surface must be considered when conducting a comprehensive analysis of the bank effect when the ship is close to the bank.
Correlation between F18-FDG PET/CT Imaging and BRAF V600E Genetic Mutation for the Early Assessment of Treatment Response in Papillary Thyroid Cancers
Authors: Piciu Andra , Larg Maria-Iulia , Piciu Doina
Published: June 2020

In thyroid neoplastic pathology, the BRAF V600E mutation is shown to be involved in the oncogenesis of papillary thyroid cancer and its subtypes. The purpose of this study is to evaluate the correlation between the mutation of the BRAF V600E oncogene and the pathological standardized uptake values (SUV) at the F18-fluorodeoxyglucose (F18-FDG) positron emission tomography/computed tomography (PET/CT) evaluation, for a group of 20 patients with radically treated (total thyroidectomy and radioiodine therapy) papillary thyroid cancer, with subclinical persistent disease, at 6 months after the initial treatment. We analyzed the correlations between the values of SUV and the presence of the BRAF mutation as well with other prognostic factors such as stage, age, specific tumor markers (thyroglobulin and anti-thyroglobulin), extrathyroid extension, the presence of metastatic lymph nodes or distant metastasis. The value of SUV in the case of BRAF+ (positive) patients was higher than in the negative ones, but without statistical significance, thus, the values of the SUV cannot be a predictable factor for the presence of the genetic mutation. There was a statistically significant correlation in BRAF+ subgroup between the SUV values and the positive resection limit following surgery, showing a higher SUV value in the PET/CT evaluation. No correlation was observed between the aforementioned prognostic factors involved in papillary thyroid cancer and the BRAF V600E mutation.
6-Hydroxy-2-methylbenzofuran-4-carboxylic Acid
Authors: Mori Matteo , Meneghetti Fiorella , Chiarelli Laurent R. , Diego Alessia , Nava Donatella , Gelain Arianna , Cazzaniga Giulia , Villa Stefania , Pini Elena
Published: June 2020

6-Hydroxy-2-methylbenzofuran-4-carboxylic acid was synthesized in two steps, starting from 3,5-dihydroxybenzoate. The product was obtained through a direct thermal one-pot cyclization with propargyl bromide, followed by a base-catalyzed hydrolysis. Its molecular structure was elucidated by means of mono- and bidimensional NMR techniques, ESI-MS, FT-IR and single-crystal X-ray diffraction.
Synergetic Effects of Hybrid Carbon Nanostructured Counter Electrodes for Dye-Sensitized Solar Cells: A Review
Authors: Samantaray Manas R. , Mondal Abhay Kumar , Murugadoss Govindhasamy , Pitchaimuthu Sudhagar , Das Santanu , Bahru Raihana , Mohamed Mohd Ambri
Published: June 2020

This article provides an overview of the structural and physicochemical properties of stable carbon-based nanomaterials and their applications as counter electrodes (CEs) in dye-sensitized solar cells (DSSCs). The research community has long sought to harvest highly efficient third-generation DSSCs by developing carbon-based CEs, which are among the most important components of DSSCs. Since the initial introduction of DSSCs, Pt-based electrodes have been commonly used as CEs owing to their high-electrocatalytic activities, thus, accelerating the redox couple at the electrode/electrolyte interface to complete the circuit. However, Pt-based electrodes have several limitations due to their cost, abundance, complicated facility, and low corrosion resistance in a liquid electrolyte, which further restricts the large-area applications of DSSCs. Although carbon-based nanostructures showed the best potential to replace Pt-CE of DSSC, several new properties and characteristics of carbon-CE have been reported for future enhancements in this field. In this review, we discuss the detailed synthesis, properties, and performances of various carbonaceous materials proposed for DSSC-CE. These nano-carbon materials include carbon nanoparticles, activated carbon, carbon nanofibers, carbon nanotube, two-dimensional graphene, and hybrid carbon material composites. Among the CE materials currently available, carbon-carbon hybridized electrodes show the best performance efficiency (up to 10.05%) with a high fill factor (83%). Indeed, up to 8.23% improvements in cell efficiency may be achieved by a carbon-metal hybrid material under sun condition. This review then provides guidance on how to choose appropriate carbon nanomaterials to improve the performance of CEs used in DSSCs.
Modeling and Experimental Study of the Localized Electrochemical Micro Additive Manufacturing Technology Based on the FluidFM
Authors: Ren Wanfei , Xu Jinkai , Lian Zhongxu , Yu Peng , Yu Huadong
Published: June 2020

In this work, the localized electrochemical micro additive manufacturing technology based on the FluidFM (fluidic force microscope) has been introduced to fabricate micro three-dimensional overhang metal structures at sub-micron resolution. It breaks through the localized deposition previously achieved by micro-anode precision movement, and the micro-injection of the electrolyte is achieved in a stable electric field distribution. The structure of electrochemical facilities has been designed and optimized. More importantly, the local electrochemical deposition process has been analyzed with positive source diffusion, and the mathematical modeling has been revealed in the particle conversion process. A mathematical model is proposed for the species flux under the action of pulsed pressure in an innovatively localized liquid feeding process. Besides, the linear structure, bulk structure, complex structure, and large-area structure of the additive manufacturing are analyzed separately. The experimental diameter of the deposited cylinder structure is linearly fitted. The aspect ratio of the structure is greater than 20, the surface roughness value is between 0.1–0.2 μm at the surface of bulk structures, and the abilities are verified for deposition of overhang, hollow complex structures. Moreover, this work verifies the feasibility of 3D overhang array submicron structure additive manufacturing, with the application of pulsed pressure. Furthermore, this technology opens new avenues for the direct fabrication of nano circuit interconnection, tiny sensors, and micro antennas.
Kuelbs–Steadman Spaces for Banach Space-Valued Measures
Authors: Boccuto Antonio , Hazarika Bipan , Kalita Hemanta
Published: June 2020

We introduce Kuelbs–Steadman-type spaces ( K S p spaces) for real-valued functions, with respect to countably additive measures, taking values in Banach spaces. We investigate the main properties and embeddings of L q -type spaces into K S p spaces, considering both the norm associated with the norm convergence of the involved integrals and that related to the weak convergence of the integrals.
The Effect of the Transformation of ε-Fe2-3N into γ′-Fe4N Phase on the Fatigue Strength of Gas-Nitrided Pure Iron
Authors: Kim Sunkwang , Yoon Sungook , Kim Jun-Ho , Park Soon
Published: June 2020

The effect of the iron nitride phases, ε-Fe2-3N and γ′-Fe4N, on the fatigue strength was investigated. Pure iron was used to observe only the effect of nitride, excluding the effects of factors, such as residual stress, depending on the alloy composition and microstructural change according to working on the fatigue strength. In this work, ε and γ′ phases were respectively grown at a time on the surface of the pure iron specimens using the appropriate nitriding potential KN, the mixture rates of ammonia and hydrogen gases, at same temperature of 570 °C according to the Fe-N Lehrer diagram. Another γ′ phase was prepared by first growing the ε phase and then transformed from ε phase into γ′ phase by changing the KN at the same temperature of 570 °C in the 2-stage gas nitriding. The fatigue strengths of the iron nitride consisted of ε and γ′ phases, γ′ phase, and γ′ phase grown by the 2-stage gas nitriding were evaluated, respectively. As a result, first, it can be seen that the diffusion layer of ε phase was deeper than γ′ phase, but fatigue strength was lower. On the other hand, fatigue strength of both the γ′ phases are higher than that of the ε, and the fatigue strength of γ′ phase nitride grown by 2-stage gas nitriding was almost similar to that of γ′ phase nitride grown at a time, i.e., fatigue strength was not significantly related to diffusion depth and depended on nitride phases in this study. Secondly, we cannot clearly conclude that there was the difference in fatigue strength according to the thickness of nitride layer consisted of γ′ phase. However, it is clear that when ε phase was transformed to γ′ phase, fatigue strength had the same level as γ′ phase formed at one time.
Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against Pathogenic Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae
Authors: Kim Hyoun Joong , Giri Sib Sankar , Kim Sang Guen , Kim Sang Wha , Kwon Jun , Lee Sung Bin , Park Se Chang
Published: June 2020

Vibrio coralliilyticus is one of the major pathogens causing mass mortality in marine bivalve larvae aquaculture. To prevent and control Vibrio spp. infections in marine bivalve hatcheries, various antibiotics are overused, resulting in environmental pollution and the creation of multi-drug-resistant strains. Therefore, research on the development of antibiotic substitutes is required. In this study, we isolated two bacteriophages (phages) that specifically infected pathogenic V. coralliilyticus from an oyster hatchery and designated them as pVco-5 and pVco-7. Both phages were classified as Podoviridae and were stable over a wide range of temperatures (4–37 °C) and at pH 7.0–9.0. Thus, both phages were suitable for application under the environmental conditions of an oyster hatchery. The two phages showed confirmed significant bactericidal efficacy against pathogenic V. coralliilyticus in an in vitro test. In the in vivo experiment, the phage pre-treated groups of Pacific oyster larvae showed significantly lower mortality against V. coralliilyticus infection than untreated control larvae. The results of the present study suggest that both phages could be used in the artificial marine bivalve seedling industry; not only to prevent pathogenic V. coralliilyticus infection, but also to reduce antibiotic overuse.
Removal Ability and Resistance to Cinnamic and Vanillic Acids by Fungi
Authors: Speranza Barbara , Cibelli Francesca , Baiano Antonietta , Carlucci Antonia , Raimondo Maria Luisa , Campaniello Daniela , Viggiani Ilaria , Bevilacqua Antonio , Rosaria Corbo Maria
Published: June 2020

Twelve fungal strains were assayed to investigate their resistance to cinnamic and vanillic acids and their ability to remove these compounds from a liquid medium. In a first step, the effect of the two aromatic acids (1 g/L) on the fungal growth kinetic was studied. The results were modelled through a logistic like function (Dantigny equation) to estimate τ, which is the time to the half-maximum colony diameter. The key findings of this part were as follows: (i) generally, cinnamic acid exerted a stronger effect than vanillic acid; (ii) aromatic acids exerted a delay on the growth of some fungi and only one strain (Athelia rolfsii) was completely inhibited. In the second part, fungi were assayed to investigate their ability to remove cinnamic and vanillic acids (ca. 350 mg/kg) from liquid media at pH 3.5. The results indicated that the most efficient fungi were Aspergillus niger and Lasiodiplodia theobromae.
Relative Abundance and Compositional Variation of Silicates, Oxides and Phosphates in the W-Sn-Rich Lodes of the Panasqueira Mine (Portugal): Implications for the Ore-Forming Process
Authors: Mateus António , Figueiras Jorge , Martins Ivo , Rodrigues Pedro , Pinto Filipe
Published: June 2020

Panasqueira is a world-class W-Sn-Cu lode-type deposit located in Portugal. It consists of a dense swarm of subhorizontal quartz lodes criss-crossed by several ENE–WSW and N–S fault zones, bordering Late Variscan granite and hosted in Late Ediacaran—Early Cambrian metasediments. The relative abundance and compositional variation (assessed with EPMA) of the main silicates, oxides and phosphates forming the quartz lodes and their margins were examined, aiming to explore: (i) mineral and geochemical zonation at the mine scale; and (ii) some conclusions on the chemical nature of prevalent fluid inflows and T-conditions of mineral deposition. Quartz lodes nearby or far from the known greisen-granite cupola display significant differences, reflecting multiple fluid influxes of somewhat distinct composition related to various opening and closing events extending for several My, ranging from an early “oxide–silicate stage” (OSS) to a “main sulfide stage” (MSS), and further on to a post-ore carbonate stage (POCS); however, a rejuvenation event occurred after MSS. The onset of OSS was placed at ca. 299 ± 5 Ma and the rejuvenation event at ca. 292 Ma. The OSS was confined to ≈500 ≤ T ≤ 320 °C, following rutile and tourmaline growth under ≈640 ≤ T ≤ 540 °C (depending on aSiO2). The rejuvenation event (≈440–450 °C) preceded a late chlorite growth (≈250–270 °C) and the progression towards POCS.
High-CO2 Modified Atmosphere Packaging with Superchilling (−1.3 °C) Inhibit Biochemical and Flavor Changes in Turbot (Scophthalmus maximus) during Storage
Authors: Mei Jun , Liu Feng , Fang Shiyuan , Lan Weiqing , Xie Jing
Published: June 2020

The effects of modified atmosphere packaging (MAP) in combination with superchilling (−1.3 °C) on the physicochemical properties, flavor retention, and organoleptic evaluation of turbot samples were investigated during 27 days storage. Results showed that high-CO2 packaging (70% or 60% CO2) combined with superchilling could reduce the productions of off-flavor compounds, including total volatile basic nitrogen (TVB-N) and ATP-related compounds. Twenty-four volatile organic compounds were determined by gas chromatography–mass spectrometry (GC/MS) during storage, including eight alcohols, 11 aldehydes, and five ketones. The relative content of off-odor volatiles, such as 1-octen-3-ol, 1-penten-3-ol, (E)-2-octenal, octanal, and 2,3-octanedione, was also reduced by high-CO2 packaging during superchilling storage. Further, 60% CO2/10% O2/30% N2 with superchilling (−1.3 °C) could retard the water migration on the basis of the water holding capacity, low field NMR, and MRI results, and maintain the quality of turbot according to organoleptic evaluation results during storage
Selectins—The Two Dr. Jekyll and Mr. Hyde Faces of Adhesion Molecules—A Review
Authors: Tvaroška Igor , Selvaraj Chandrabose , Koča Jaroslav
Published: June 2020

Selectins belong to a group of adhesion molecules that fulfill an essential role in immune and inflammatory responses and tissue healing. Selectins are glycoproteins that decode the information carried by glycan structures, and non-covalent interactions of selectins with these glycan structures mediate biological processes. The sialylated and fucosylated tetrasaccharide sLex is an essential glycan recognized by selectins. Several glycosyltransferases are responsible for the biosynthesis of the sLex tetrasaccharide. Selectins are involved in a sequence of interactions of circulated leukocytes with endothelial cells in the blood called the adhesion cascade. Recently, it has become evident that cancer cells utilize a similar adhesion cascade to promote metastases. However, like Dr. Jekyll and Mr. Hyde’s two faces, selectins also contribute to tissue destruction during some infections and inflammatory diseases. The most prominent function of selectins is associated with the initial stage of the leukocyte adhesion cascade, in which selectin binding enables tethering and rolling. The first adhesive event occurs through specific non-covalent interactions between selectins and their ligands, with glycans functioning as an interface between leukocytes or cancer cells and the endothelium. Targeting these interactions remains a principal strategy aimed at developing new therapies for the treatment of immune and inflammatory disorders and cancer. In this review, we will survey the significant contributions to and the current status of the understanding of the structure of selectins and the role of selectins in various biological processes. The potential of selectins and their ligands as therapeutic targets in chronic and acute inflammatory diseases and cancer will also be discussed. We will emphasize the structural characteristic of selectins and the catalytic mechanisms of glycosyltransferases involved in the biosynthesis of glycan recognition determinants. Furthermore, recent achievements in the synthesis of selectin inhibitors will be reviewed with a focus on the various strategies used for the development of glycosyltransferase inhibitors, including substrate analog inhibitors and transition state analog inhibitors, which are based on knowledge of the catalytic mechanism.
Hairy Garlic (Allium subhirsutum) from Sicily (Italy): LC-DAD-MSn Analysis of Secondary Metabolites and In Vitro Biological Properties
Authors: Sut Stefania , Maggi Filippo , Bruno Sara , Badalamenti Natale , Quassinti Luana , Bramucci Massimo , Beghelli Daniela , Lupidi Giulio , Dall’Acqua Stefano
Published: June 2020

Allium subhirsutum, known as hairy garlic, is a bulbous plant widespread in the Mediterranean area and locally used as a food and spice. In the present study, the chemical profile of the ethanolic extracts from bulbs (BE) and aerial parts (APE) were analyzed by HPLC-ESI-MSn, and antioxidant properties were evaluated by DPPH, ABTS and TEAC assays. The traditional use in the diet, and the well documented biological activity of Allium species suggest a potential as a new nutraceutical. For this reason, the potential usefulness of this food can be considered in the treatment and prevention of degenerative Alzheimer disease. For this reason, acetylcholinesterase inhibitory property was investigated. Furthermore, due to the observed presence of sulfur-containing and phenolic constituents, the cytotoxicity on tumor cells line was investigated. Results revealed significant AChE inhibitory activity for BE and APE. Both extracts exhibited also moderate antioxidant properties in the in vitro assays. Finally, limited cytotoxic activity was observed towards Human colon carcinoma and adenocarcinoma cell line, with differences between the individual parts tested. HPLC-ESI-MSn analysis showed that hairy garlic is a good source of sulphur compounds, flavonoids and phenylpropanoids derivatives, thus being a valid alternative to the common garlic (A. sativum). This work opens new opportunities for the application of A. subhirsutum as a health-promoting food.
Semantic Unsupervised Automatic Keyphrases Extraction by Integrating Word Embedding with Clustering Methods
Authors: Gagliardi Isabella , Artese Maria Teresa
Published: June 2020

Increasingly, the web produces massive volumes of texts, alone or associated with images, videos, photographs, together with some metadata, indispensable for their finding and retrieval. Keywords/keyphrases that characterize the semantic content of documents should be, automatically or manually, extracted, and/or associated with them. The paper presents a novel method to address the problem of the automatic unsupervised extraction of keywords/phrases from texts, expressed both in English and in Italian. The main feature of this approach is the integration of two methods that have given interesting results: word embedding models, such as Word2Vec or GloVe able to capture the semantics of words and their context, and clustering algorithms, able to identify the essence of the terms and choose the more significant one(s), to represent the contents of a text. In the paper, the datasets used are presented, together with the method implemented and the results obtained. These results will be discussed, commented, and compared with those obtained in previous experimentations, using TextRank, Rapid Automatic Keyword Extraction (RAKE), and TF-IDF.