bluuebunny/crossref_metadata_2025 huggingface dataset:
The Influence of Severe Plastic Deformation and Subsequent Annealing on the Microstructure and Hardness of a Cu–Cr–Zr Alloy
Authors: Kapoor Garima , Kvackaj Tibor , Heczel Anita , Bidulská Jana , Kočiško Róbert , Fogarassy Zsolt , Simcak Dusan , Gubicza Jenő
Published: May 2020

A Cu–1.1%Cr–0.04%Zr (wt.%) alloy was processed by severe plastic deformation (SPD) using the equal channel angular pressing (ECAP) technique at room temperature (RT). It was found that when the number of passes increased from one to four, the dislocation density significantly increased by 35% while the crystallite size decreased by 32%. Subsequent rolling at RT did not influence considerably the crystallite size and dislocation density. At the same time, cryorolling at liquid nitrogen temperature yielded a much higher dislocation density. All the samples contained Cr particles with an average size of 1 µm. Both the size and fraction of the Cr particles did not change during the increase in ECAP passes and the application of rolling after ECAP. The hardness of the severely deformed Cu alloy samples can be well correlated to the dislocation density using the Taylor equation. Heat treatment at 430 °C for 30 min in air caused a significant reduction in the dislocation density for all the deformed samples, while the hardness considerably increased. This apparent contradiction can be explained by the solute oxygen hardening, but the annihilation of mobile dislocations during annealing may also contribute to hardening.
Effect of Ageing on the Tracking Characteristics of High-Temperature Vulcanized Silicone Rubber Hybrid Composites for High Voltage Insulation
Authors: Ghouse Shaik Mohamed , Karuppaiyan Vijayarekha
Published: May 2020

Micro-sized aluminum trihydrate (ATH) filled silicone rubber is useful as insulation in the electric power system. The addition of nanofillers can improve further, its overall performance. However, the degradation of such silicone insulator due to ageing has not yet been thoroughly analysed. Motivated by this, an investigation was done to observe the effect of ageing on the tracking, and the material characteristics of SiO2 nanofillers added micro-sized ATH-filled Silicone rubber. For this, the samples were prepared using different weight percentage of SiO2 nanofillers and were thermally-aged and water-aged in the laboratory environment. A well-regulated tracking-test setup was assembled, and the leakage current characteristics of the fresh, thermal-aged and water-aged samples were observed, as per international electro technical commission standard (IEC) 60,587. After tracking, the surface morphology of these samples was studied using scanning electron microscopy (SEM). Further, energy dispersive X-ray analysis (EDAX) was carried out to observe the elements present at the surface and, Fourier transform infra-red (FTIR) spectroscopy was conducted to study the changes in the chemical structure. Investigations through the leakage current, SEM, EDAX and FTIR revealed that the addition of nanofillers improved the tracking characteristics of the aged hybrid composite insulation samples, thereby minimising any early failures.
Flexural Strengthening of Concrete Slab-Type Elements with Textile Reinforced Concrete
Authors: Kim Hyeong-Yeol , You Young-Jun , Ryu Gum-Sung , Koh Kyung-Taek , Ahn Gi-Hong , Kang Se-Hoon
Published: May 2020

This paper deals with flexural strengthening of reinforced concrete (RC) slabs with a carbon textile reinforced concrete (TRC) system. The surface coating treatment was applied to a carbon grid-type textile to increase the bond strength. Short fibers were incorporated into the matrix to mitigate the formation of shrinkage-induced cracks. The tensile properties of the TRC system were evaluated by a direct tensile test with a dumbbell-type grip method. The tensile test results indicated that the effect of the surface coating treatment of the textile on the bonding behavior of the textile within the TRC system was significant. Furthermore, the incorporation of short fibers in the matrix was effective to mitigate shrinkage-induced crack formation and to improve the tensile properties of the TRC system. Six full-scale slab specimens were strengthened with the TRC system and, subsequently, failure tested. The ultimate load-carrying capacity of the strengthened slabs was compared with that of an unstrengthened slab as well as the theoretical solutions. The failure test results indicated that the stiffness and the ultimate flexural capacity of the strengthened slab were at least 112% and 165% greater, respectively, than that of the unstrengthened slab. The test results further indicated that the strengthening effect was not linearly proportional to the amount of textile reinforcement.
Using In Situ Polymerization to Increase Puncture Resistance and Induce Reversible Formability in Silk Membranes
Authors: Emonson Nicholas S. , Eyckens Daniel J. , Allardyce Benjamin J. , Hendlmeier Andreas , Stanfield Melissa K. , Soulsby Lachlan C. , Stojcevski Filip , Henderson Luke C.
Published: May 2020

Silk fibroin is an excellent biopolymer for application in a variety of areas, such as textiles, medicine, composites and as a novel material for additive manufacturing. In this work, silk membranes were surface modified by in situ polymerization of aqueous acrylic acid, initiated by the reduction of various aryldiazonium salts with vitamin C. Treatment times of 20 min gave membranes which possessed increased tensile strength, tensile modulus, and showed significant increased resistance to needle puncture (+131%), relative to ‘untreated’ standards. Most interestingly, the treated silk membranes were able to be reversibly formed into various shapes via the hydration and plasticizing of the surface bound poly(acrylic acid), by simply steaming the modified membranes. These membranes and their unique properties have potential applications in advanced textiles, and as medical materials.
Mechanical Behavior of Printed Strain Hardening Cementitious Composites
Authors: Chaves Figueiredo Stefan , Romero Rodríguez Claudia , Y. Ahmed Zeeshan , Bos Derk H. , Xu Yading , Salet Theo M. , Çopuroğlu Oğuzhan , Schlangen Erik , Bos Freek P.
Published: May 2020

Extrusion based additive manufacturing of cementitious materials has demonstrated strong potential to become widely used in the construction industry. However, the use of this technique in practice is conditioned by a feasible solution to implement reinforcement in such automated process. One of the most successful ductile materials in civil engineering, strain hardening cementitious composites (SHCC) have a high potential to be employed for three-dimensional printing. The match between the tailored brittle matrix and ductility of the fibres enables these composites to develop multiple cracks when loaded under tension. Using previously developed mixtures, this study investigates the physical and mechanical performance of printed SHCC. The anisotropic behavior of the materials is explored by means of mechanical tests in several directions and micro computed tomography tests. The results demonstrated a composite showing strain hardening behavior in two directions explained by the fibre orientation found in the printed elements. Moreover, the printing technique used also has guaranteed an enhanced bond in between the printed layers.
Multidisciplinary Design Optimization of a Novel Sandwich Beam-Based Adaptive Tuned Vibration Absorber Featuring Magnetorheological Elastomer
Authors: Asadi Khanouki Mostafa , Sedaghati Ramin , Hemmatian Masoud
Published: May 2020

The present study aims to investigate the dynamic performance and design optimization of a novel magnetorheological elastomer based adaptive tuned vibration absorber (MRE-ATVA). The proposed MRE-ATVA consists of a light-weight sandwich beam treated with an MRE core layer and two electromagnets installed at both free ends. Three different design configurations for electromagnets are proposed. The finite element (FE) model of the proposed MRE-ATVA and magnetic model of the electromagnets are developed and combined to evaluate the frequency range of the absorber under varying magnetic field intensity. The results of the developed model are validated in multiple stages with available analytical and simulation data. A multidisciplinary design optimization strategy has been formulated to maximize the frequency range of the proposed MRE-based ATVA while respecting constraints of weight, size, mechanical stress, and sandwich beam deflection. The optimal solution is obtained and compared for the three proposed ATVA configurations. The optimal ATVA with a U-shaped electromagnet shows more than 40% increase in the natural frequency while having a total mass of 596 g.
Hydrogen Concentration Distribution in 2.25Cr-1Mo-0.25V Steel under the Electrochemical Hydrogen Charging and Its Influence on the Mechanical Properties
Authors: Yin Changdong , Chen Jianjun , Ye Dongdong , Xu Zhou , Ge Jiahao , Zhou Haiting
Published: May 2020

The deterioration of the mechanical properties of metal induced by hydrogen absorption threatens the safety of the equipment serviced in hydrogen environments. In this study, the hydrogen concentration distribution in 2.25Cr-1Mo-0.25V steel after hydrogen charging was analyzed following the hydrogen permeation and diffusion model. The diffusible hydrogen content in the 1-mm-thick specimen and its influence on the mechanical properties of the material were investigated by glycerol gas collecting test, static hydrogen charging tensile test, scanning electron microscopy (SEM) test, and microhardness test. The results indicate that the content of diffusible hydrogen tends to be the saturation state when the hydrogen charging time reaches 48 h. The simulation results suggest that the hydrogen concentration distribution can be effectively simulated by ABAQUS and the method can be used to analyze the hydrogen concentration in the material with complex structures or containing multiple microstructures. The influence of hydrogen on the mechanical properties is that the elongation of this material is reduced and the diffusible hydrogen will cause a decrease in the fracture toughness of the material, and thus hydrogen embrittlement (HE) will occur. Moreover, the Young’s modulus E and microhardness are increased due to hydrogen absorption, and the variation value is related to the hydrogen concentration introduced into the specimen.
Virtual Testing of Counterbalance Forklift Trucks: Implementation and Experimental Validation of a Numerical Multibody Model
Authors: Martini Alberto , Bonelli Giovanni Paolo , Rivola Alessandro
Published: May 2020

This study investigates the dynamic behavior of a recently developed counterbalance forklift truck. The final objective is creating virtual testing tools based on numerical multibody models to evaluate the dynamic stresses experienced by the forklift family of interest during a reference operating cycle, defined by the manufacturer’s testing protocols. This work aims at defining sufficiently accurate and easy-to-implement modelling approaches and validation procedures. It focuses on a specific test, namely the passage of a speed-bump-like obstacle at high velocity, which represents one of the most severe conditions within the reference cycle. Indeed, unlike most of the other wheeled vehicles, forklifts typically do not have advanced suspension systems and their dynamic response is significantly affected by ground irregularities. To this end, a preliminary model of the complete forklift, featuring rigid bodies and a simplified tire–ground contact model, is implemented with a commercial software. Experimental tests are conducted on the forklift to measure the vehicle vibrations when running on the obstacle, for model validation purposes. After model updating, the results provided by the numerical simulations match the experimental data satisfactorily. Hence, the modelling and validation strategies are proven viable and effective.
Subclasses of Bi-Univalent Functions Defined by Frasin Differential Operator
Authors: Aldawish Ibtisam , Al-Hawary Tariq , Frasin B. A.
Published: May 2020

Let Ω denote the class of functions f ( z ) = z + a 2 z 2 + a 3 z 3 + ⋯ belonging to the normalized analytic function class A in the open unit disk U = z : z < 1 , which are bi-univalent in U , that is, both the function f and its inverse f − 1 are univalent in U . In this paper, we introduce and investigate two new subclasses of the function class Ω of bi-univalent functions defined in the open unit disc U , which are associated with a new differential operator of analytic functions involving binomial series. Furthermore, we find estimates on the Taylor–Maclaurin coefficients | a 2 | and | a 3 | for functions in these new subclasses. Several (known or new) consequences of the results are also pointed out.
Unsteady Three-Dimensional MHD Non-Axisymmetric Homann Stagnation Point Flow of a Hybrid Nanofluid with Stability Analysis
Authors: Zainal Nurul Amira , Nazar Roslinda , Naganthran Kohilavani , Pop Ioan
Published: May 2020

The hybrid nanofluid under the influence of magnetohydrodynamics (MHD) is a new interest in the industrial sector due to its applications, such as in solar water heating and scraped surface heat exchangers. Thus, the present study accentuates the analysis of an unsteady three-dimensional MHD non-axisymmetric Homann stagnation point flow of a hybrid Al2O3-Cu/H2O nanofluid with stability analysis. By employing suitable similarity transformations, the governing mathematical model in the form of the partial differential equations are simplified into a system of ordinary differential equations. The simplified mathematical model is then solved numerically by the Matlab solver bvp4c function. This solving approach was proficient in generating more than one solution when good initial guesses were provided. The numerical results presented significant influences on the rate of heat transfer and fluid flow characteristics of a hybrid nanofluid. The rate of heat transfer and the trend of the skin friction coefficient improve with the increment of the nanoparticles’ concentration and the magnetic parameter; however, they deteriorate when the unsteadiness parameter increases. In contrast, the ratio of the escalation of the ambient fluid strain rate to the plate was able to adjourn the boundary layer separation. The dual solutions (first and second solutions) are obtainable when the surface of the sheet shrunk. A stability analysis is carried out to justify the stability of the dual solutions, and hence the first solution is seen as physically reliable and stable, while the second solution is unstable.
Parameter Estimation and Measurement of Social Inequality in a Kinetic Model for Wealth Distribution
Authors: Buffa Bruno Adolfo , Knopoff Damián , Torres Germán
Published: May 2020

This paper deals with the modeling of wealth distribution considering a society with non-constant population and non-conservative wealth trades. The modeling approach is based on the kinetic theory of active particles, where individuals are distinguished by a scalar variable (the activity) which expresses their social state. A qualitative analysis of the model focusing on asymptotic behaviors and measurement of inequality through the Gini coefficient is presented. Finally, some specific case-studies are proposed in order to carry out numerical experiments to validate our model, characterize societies and investigate emerging behaviors.
Software Reliability for Agile Testing
Authors: van Driel Willem Dirk , Bikker Jan Willem , Tijink Matthijs , Di Bucchianico Alessandro
Published: May 2020

It is known that quantitative measures for the reliability of software systems can be derived from software reliability models, and, as such, support the product development process. Over the past four decades, research activities in this area have been performed. As a result, many software reliability models have been proposed. It was shown that, once these models reach a certain level of convergence, it can enable the developer to release the software and stop software testing accordingly. Criteria to determine the optimal testing time include the number of remaining errors, failure rate, reliability requirements, or total system cost. In this paper, we present our results in predicting the reliability of software for agile testing environments. We seek to model this way of working by extending the Jelinski–Moranda model to a “stack” of feature-specific models, assuming that the bugs are labeled with the features they belong to. In order to demonstrate the extended model, two use cases are presented. The questions to be answered in these two cases are: how many software bugs remain in the software and should one decide to stop testing the software?
Fast Imaging of Thin, Curve-Like Electromagnetic Inhomogeneities without a Priori Information
Authors: Park Won-Kwang
Published: May 2020

It is well-known that subspace migration is a stable and effective non-iterative imaging technique in inverse scattering problem. However, for a proper application, a priori information of the shape of target must be estimated. Without this consideration, one cannot retrieve good results via subspace migration. In this paper, we identify the mathematical structure of single- and multi-frequency subspace migration without any a priori of unknown targets and explore its certain properties. This is based on the fact that elements of so-called multi-static response (MSR) matrix can be represented as an asymptotic expansion formula. Furthermore, based on the examined structure, we improve subspace migration and consider the multi-frequency subspace migration. Various results of numerical simulation with noisy data support our investigation.
Queuing System with Unreliable Servers and Inhomogeneous Intensities for Analyzing the Impact of Non-Stationarity to Performance Measures of Wireless Network under Licensed Shared Access
Authors: Markova Ekaterina , Satin Yacov , Kochetkova Irina , Zeifman Alexander , Sinitcina Anna
Published: May 2020

Given the limited frequency band resources and increasing volume of data traffic in modern multiservice networks, finding new and more efficient radio resource management (RRM) mechanisms is becoming indispensable. One of the implemented technologies to solve this problem is the licensed shared access (LSA) technology. LSA allows the spectrum that has been licensed to an owner, who has absolute priority on its utilization, to be used by other participants (i.e., tenants). Owner priority impacts negatively on the quality of service (QoS) by reducing the data bit rate and interrupting user services. In this paper, we propose a wireless multiservice network scheme model described as a queuing system with unreliable servers and a finite buffer within the LSA framework. The aim of this work is to analyze main system performance measures: blocking probability, average number of requests in queue, and average queue length depending on LSA frequencies’ availability.
Cytotoxic Scalarane Sesterterpenes from the Sponge Hyrtios erectus
Authors: Kwon Oh-Seok , Kim Donghwa , Kim Chang-Kwon , Sun Jeongyoon , Sim Chung J. , Oh Dong-Chan , Lee Sang Kook , Oh Ki-Bong , Shin Jongheon
Published: May 2020

Twelve new sesterterpenes along with eight known sesterterpenes were isolated from the marine sponge Hyrtios erectus collected off the coast of Chuuk Island, the Federated State of Micronesia. Based upon a combination of spectroscopic and computational analyses, these compounds were determined to be eight glycine-bearing scalaranes (1–8), a 3-keto scalarane (9), two oxidized-furan-bearing scalaranes (10 and 11), and a salmahyrtisane (12). Several of these compounds exhibited weak antiproliferation against diverse cancer cell lines as well as moderate anti-angiogenesis activities. The antiproliferative activity of new compound 4 was found to be associated with G0/G1 arrest in the cell cycle.
Bioactive Molecules from Mangrove Streptomyces qinglanensis 172205
Authors: Xu Dongbo , Tian Erli , Kong Fandong , Hong Kui
Published: May 2020

Five new compounds 15R-17,18-dehydroxantholipin (1), (3E,5E,7E)-3-methyldeca-3,5,7-triene-2,9-dione (2) and qinlactone A–C (3–5) were identified from mangrove Streptomyces qinglanensis 172205 with “genetic dereplication,” which deleted the highly expressed secondary metabolite-enterocin biosynthetic gene cluster. The chemical structures were established by spectroscopic methods, and the absolute configurations were determined by electronic circular dichroism (ECD). Compound 1 exhibited strong anti-microbial and antiproliferative bioactivities, while compounds 2–4 showed weak antiproliferative activities.
Pheophorbide a: State of the Art
Authors: Saide Assunta , Lauritano Chiara , Ianora Adrianna
Published: May 2020

Chlorophyll breakdown products are usually studied for their antioxidant and anti-inflammatory activities. The chlorophyll derivative Pheophorbide a (PPBa) is a photosensitizer that can induce significant anti-proliferative effects in several human cancer cell lines. Cancer is a leading cause of death worldwide, accounting for about 9.6 million deaths, in 2018 alone. Hence, it is crucial to monitor emergent compounds that show significant anticancer activity and advance them into clinical trials. In this review, we analyze the anticancer activity of PPBa with or without photodynamic therapy and also conjugated with or without other chemotherapic drugs, highlighting the capacity of PPBa to overcome multidrug resistance. We also report other activities of PPBa and different pathways that it can activate, showing its possible applications for the treatment of human pathologies.
Should Autism Spectrum Conditions Be Characterised in a More Positive Way in Our Modern World?
Authors: Wright Barry , Spikins Penny , Pearson Hannah
Published: May 2020

In a special issue that focuses on complex presentations related to Autism, we ask the question in this editorial whether an Autism Spectrum Condition without complexity is a disorder, or whether it represents human diversity? Much research into Autism Spectrum Conditions (ASCs) over the years has focused on comparisons between neuro-typical people and people with Autism Spectrum Conditions. These comparisons have tended to draw attention to ‘deficits’ in cognitive abilities and descriptions of behaviours that are characterised as unwanted. Not surprisingly, this is reflected in the classification systems from the World Health Organisation and the American Psychiatric Association. Public opinion about ASC may be influenced by presentations in the media of those with ASC who also have intellectual disability. Given that diagnostic systems are intended to help us better understand conditions in order to seek improved outcomes, we propose a more constructive approach to descriptions that uses more positive language, and balances descriptions of deficits with research finding of strengths and differences. We propose that this will be more helpful to individuals on the Autism Spectrum, both in terms of individual self-view, but also in terms of how society views Autism Spectrum Conditions more positively. Commentary has also been made on guidance that has been adjusted for people with ASC in relation to the current COVID-19 pandemic.
Effects of Khat on Surface Roughness and Color of Feldspathic and Zirconia Porcelain Materials under Simulated Oral Cavity Conditions
Authors: Al Moaleem Mohammed M. , AlSanosy Rashad , Al Ahmari Nasser M. , Shariff Mansoor , Alshadidi Abdulkhaliq A. , Alhazmi Hassan A. , Khalid Asaad
Published: May 2020

Background and Objectives: Khat chewing is considered as a daily habit that is practiced by more than five million people globally. The effect of khat chewing on the surface roughness and the color stability of natural teeth and the material used in the fabrication of dental prosthesis remains unknown. This study was conducted to explore and compare the effect of khat homogenate (KH) on the surface roughness (Ra) and the average color changes (ΔE*) amongst natural teeth and selected shades from different porcelain types, namely, feldspathic metal ceramic (MC) VM13, computer-aided design/computer assisted manufacture (CAD/CAM) feldspathic (Vitablocs Mark II), and multilayer zirconia (Ceramill Zolid PS) porcelains. Materials and Methods: Seventy samples were prepared from natural teeth, feldspathic MC, CAD/CAM Vitablocs Mark II, and zirconia porcelain. The Ra values were measured using a profilometer and expressed in micrometers, whereas the ΔE* values were measured using VITA Easyshade® V spectrophotometer for all samples before and after frequent immersion and thermocycling in KH for 30 days. The surface topography was used to assess the materials surfaces (glazed or polished) after KH immersion by using a white light interferometry machine. Results: Results revealed that the Ra and the ΔE* values of the different types of tested porcelain were influenced by KH. The order of surface roughness values was glazed or polished MC > polished Zircon > polished Vitablocs Mark II > natural teeth. The lowest ΔE* values were recorded for glazed Vitablocs Mark II and MC, and the values could be arranged as polished zircon > natural teeth > glazed zircon > polished MC > polished Vitablocs Mark II. P values were significantly varied (<0.001) among all the tested groups, except the zircon group (>0.05) for both Ra and ΔE*. Conclusions: KH significantly affected both surface parameter and color of glazed or polished porcelain materials and natural teeth.
New Perspectives on Fuel Cell Technology: A Brief Review
Authors: Sazali Norazlianie , Wan Salleh Wan Norharyati , Jamaludin Ahmad Shahir , Mhd Razali Mohd Nizar
Published: May 2020

Energy storage and conversion is a very important link between the steps of energy production and energy consumption. Traditional fossil fuels are a natural and unsustainable energy storage medium with limited reserves and notorious pollution problems, therefore demanding a better choice to store and utilize the green and renewable energies in the future. Energy and environmental problems require a clean and efficient way of using the fuels. Fuel cell functions to efficiently convert oxidant and chemical energy accumulated in the fuel directly into DC electric, with the by-products of heat and water. Fuel cells, which are known as effective electrochemical converters, and electricity generation technology has gained attention due to the need for clean energy, the limitation of fossil fuel resources and the capability of a fuel cell to generate electricity without involving any moving mechanical part. The fuel cell technologies that received high interest for commercialization are polymer electrolyte membrane fuel cells (PEMFCs), solid oxide fuel cells (SOFCs), and direct methanol fuel cells (DMFCs). The optimum efficiency for the fuel cell is not bound by the principle of Carnot cycle compared to other traditional power machines that are generally based on thermal cycles such as gas turbines, steam turbines and internal combustion engines. However, the fuel cell applications have been restrained by the high cost needed to commercialize them. Researchers currently focus on the discovery of different materials and manufacturing methods to enhance fuel cell performance and simplify components of fuel cells. Fuel cell systems’ designs are utilized to reduce the costs of the membrane and improve cell efficiency, durability and reliability, allowing them to compete with the traditional combustion engine. In this review, we primarily analyze recent developments in fuel cells technologies and up-to-date modeling for PEMFCs, SOFCs and DMFCs.
Minimum Net Driving Temperature Concept for Membrane Distillation
Authors: Blankert Bastiaan , Vrouwenvelder Johannes S. , Witkamp Geert-Jan , Ghaffour Noreddine
Published: May 2020

In this study, we analyzed the heat requirement of membrane distillation (MD) to investigate the trade-off between the evaporation efficiency and driving force efficiency in a single effect MD system. We found that there exists a non-zero net driving temperature difference that maximizes efficiency. This is the minimum net driving temperature difference necessary for a rational operational strategy because below the minimum net driving temperature, both the productivity and efficiency can be increased by increasing the temperature difference. The minimum net driving temperature has a similar magnitude to the boiling point elevation (~0.5 °C for seawater), and depends on the properties of the membrane and the heat exchanger. The minimum net driving temperature difference concept can be used to understand the occurrence of optimal values of other parameters, such as flux, membrane thickness, and membrane length, if these parameters are varied in a way that consequently varies the net driving temperature difference.
The Role of New Inorganic Materials in Composite Membranes for Water Disinfection
Authors: Castro-Muñoz Roberto
Published: May 2020

Today, there is an increasing interest in improving the physicochemical properties of polymeric membranes by merging the membranes with different inorganic materials. These so-called composite membranes have been implemented in different membrane-based technologies (e.g., microfiltration, ultrafiltration, nanofiltration, membrane bioreactors, among others) for water treatment and disinfection. This is because such inorganic materials (such as TiO2-, ZnO-, Ag-, and Cu-based nanoparticles, carbon-based materials, to mention just a few) can improve the separation performance of membranes and also some other properties, such as antifouling, mechanical, thermal, and physical and chemical stability. Moreover, such materials display specific biological activity towards viruses, bacteria, and protozoa, showing enhanced water disinfection properties. Therefore, the aim of this review is to collect the latest advances (in the last five years) in using composite membranes and new hybrid materials for water disinfection, paying particular emphasis on relevant results and new hydride composites together with their preparation protocols. Moreover, this review addresses the main mechanism of action of different conventional and novel inorganic materials toward biologically active matter.
Microstructural Evolutions and its Impact on the Corrosion Behaviour of Explosively Welded Al/Cu Bimetal
Authors: Jandaghi Mohammad Reza , Saboori Abdollah , Khalaj Gholamreza , Khanzadeh Ghareh Shiran Mohammadreza
Published: May 2020

In this study, the microstructural evolutions and corrosion resistance of aluminium/copper joint fabricated through explosive welding process have been thoroughly investigated, while stand-off distance was variable. Microstructural analyses demonstrate that, regardless of grain refinement in the welding boundary, increasing the stand-off space is followed by a higher thickness of the localized melting pool. X-Ray diffraction (XRD) and energy-dispersive X-ray spectroscopy (EDS) analyses recognized the binary intermetallic layers as a combination of Al2Cu and AlCu. Polarization and electrochemical impedance spectroscopy (EIS) corrosion tests revealed that a higher stand-off distance resulted in the increment of corrosion potential, current rate, and concentration gradient at the interface owing to the remarkable kinetic energy of the collision, which impaired corrosion resistance.
The Effect of Bee Venom Peptides Melittin, Tertiapin, and Apamin on the Human Erythrocytes Ghosts: A Preliminary Study
Authors: Światły-Błaszkiewicz Agata , Mrówczyńska Lucyna , Matuszewska Eliza , Lubawy Jan , Urbański Arkadiusz , Kokot Zenon J. , Rosiński Grzegorz , Matysiak Jan
Published: May 2020

Red blood cells (RBCs) are the most abundant cells in the human blood that have been extensively studied under morphology, ultrastructure, biochemical and molecular functions. Therefore, RBCs are excellent cell models in the study of biologically active compounds like drugs and toxins on the structure and function of the cell membrane. The aim of the present study was to explore erythrocyte ghost’s proteome to identify changes occurring under the influence of three bee venom peptides-melittin, tertiapin, and apamin. We conducted preliminary experiments on the erythrocyte ghosts incubated with these peptides at their non-hemolytic concentrations. Such preparations were analyzed using liquid chromatography coupled with tandem mass spectrometry. It was found that when higher concentrations of melittin and apamin were used, fewer proteins were identified. Moreover, the results clearly indicated that apamin demonstrates the greatest influence on the RBCs ghosts proteome. Interestingly, the data also suggest that tertiapin exerted a stabilizing effect on the erythrocyte membrane. The experiments carried out show the great potential of proteomic research in the projects focused on the toxin’s properties as membrane active agents. However, to determine the specificity of the effect of selected bee venom peptides on the erythrocyte ghosts, further proteomic research should be focused on the quantitative analysis.
Metabolomics Analysis Reveals Global Metabolic Changes in the Evolved E. coli Strain with Improved Growth and 1-Butanol Production in Minimal Medium
Authors: Laviña Walter A. , Sakurai Sana Subhan Memon , Pontrelli Sammy , Putri Sastia Prama , Fukusaki Eiichiro
Published: May 2020

Production of 1-butanol from microorganisms has garnered significant interest due to its prospect as a drop-in biofuel and precursor for a variety of commercially relevant chemicals. Previously, high 1-butanol titer has been reported in Escherichia coli strain JCL166, which contains a modified clostridial 1-butanol pathway. Although conventional and metabolomics-based strain improvement strategies of E. coli strain JCL166 have been successful in improving production in rich medium, 1-butanol titer was severely limited in minimal medium. To further improve growth and consequently 1-butanol production in minimal medium, adaptive laboratory evolution (ALE) using mutD5 mutator plasmid was done on JCL166. Comparative metabolomics analysis of JCL166 and BP1 revealed global perturbations in the evolved strain BP1 compared to JCL166 (44 out of 64 metabolites), encompassing major metabolic pathways such as glycolysis, nucleotide biosynthesis, and CoA-related processes. Collectively, these metabolic changes in BP1 result in improved growth and, consequently, 1-butanol production in minimal medium. Furthermore, we found that the mutation in ihfB caused by ALE had a significant effect on the metabolome profile of the evolved strain. This study demonstrates how metabolomics was utilized for characterization of ALE-developed strains to understand the overall effect of mutations acquired through evolution.
Role of the Mitochondrial Citrate-malate Shuttle in Hras12V-Induced Hepatocarcinogenesis: A Metabolomics-Based Analysis
Authors: Lei Chuanyi , Chen Jun , Li Huiling , Fan Tingting , Zheng Xu , Wang Hong , Zhang Nan , Liu Yang , Luo Xiaoqin , Wang Jingyu , Wang Aiguo
Published: May 2020

The activation of the Ras signaling pathway is a crucial process in hepatocarcinogenesis. Till now, no reports have scrutinized the role of dynamic metabolic changes in Ras oncogene-induced transition of the normal and precancerous liver cells to hepatocellular carcinoma in vivo. In the current study, we attempted a comprehensive investigation of Hras12V transgenic mice (Ras-Tg) by concatenating nontargeted metabolomics, transcriptomics analysis, and targeted-metabolomics incorporating [U-13C] glucose. A total of 631 peaks were detected, out of which 555 metabolites were screened. Besides, a total of 122 differently expressed metabolites (DEMs) were identified, and they were categorized and subtyped with the help of variation tendency analysis of the normal (W), precancerous (P), and hepatocellular carcinoma (T) liver tissues. Thus, the positive or negative association between metabolites and the hepatocellular carcinoma and Ras oncogene were identified. The bioinformatics analysis elucidated the hepatocarcinogenesis-associated significant metabolic pathways: glycolysis, mitochondrial citrate-malate shuttle, lipid biosynthesis, pentose phosphate pathway (PPP), cholesterol and bile acid biosynthesis, and glutathione metabolism. The key metabolites and enzymes identified in this analysis were further validated. Moreover, we confirmed the PPP, glycolysis, and conversion of pyruvate to cytosol acetyl-CoA by mitochondrial citrate-malate shuttle, in vivo, by incorporating [U-13C] glucose. In summary, the current study presented the comprehensive bioinformatics analysis, depicting the Ras oncogene-induced dynamic metabolite variations in hepatocarcinogenesis. A significant finding of our study was that the mitochondrial citrate-malate shuttle plays a crucial role in detoxification of lactic acid, maintenance of mitochondrial integrity, and enhancement of lipid biosynthesis, which, in turn, promotes hepatocarcinogenesis.
Dyslipidemia: A Trigger for Coronary Heart Disease in Romanian Patients with Diabetes
Authors: Găman Mihnea-Alexandru , Cozma Matei-Alexandru , Dobrică Elena-Codruța , Bacalbașa Nicolae , Bratu Ovidiu Gabriel , Diaconu Camelia Cristina
Published: May 2020

Previous studies have reported age and gender disparities in the occurrence and therapeutic approach of dyslipidemia and (or) coronary heart disease (CHD) in patients with type 2 diabetes mellitus (T2DM). We aimed to investigate these differences in Romanian patients with T2DM. A cross-sectional, observational, retrospective study was conducted using the medical records of T2DM patients who attended the outpatient facility of the Internal Medicine Clinic of the Clinical Emergency Hospital of Bucharest, Romania for routine check-ups in a six-month period. We analyzed the records of 217 diabetic patients (mean age 69 ± 11 years; 51.15% women). We found no significant gender differences in the occurrence of dyslipidemia, CHD or CHD + dyslipidemia or in terms of statin prescription. However; patients aged 65 years or older were significantly more affected by dyslipidemia, CHD or CHD + dyslipidemia, versus subjects aged <65 years. Further, they were more likely to be prescribed statin therapy (p < 0.0001 for all). Statins were prescribed to 67.24% of the patients with dyslipidemia; 61.01% of the subjects with CHD; and to 91.48% of the patients who had both conditions. e recorded no gender differences in the occurrence of CHD and (or) dyslipidemia in Romanian T2DM patients. Patients aged 65 years or older had a higher prevalence of CHD and/or dyslipidemia, and were more likely to be prescribed statins, versus younger counterparts. However, many T2DM patients with CHD and (or) dyslipidemia were undertreated: Nearly 33% of the subjects with dyslipidemia, and nearly 40% of the ones with CHD were not prescribed statins.
Photocatalytic Nanofabrication and Intracellular Raman Imaging of Living Cells with Functionalized AFM Probes
Authors: Shibata Takayuki , Furukawa Hiromi , Ito Yasuharu , Nagahama Masahiro , Hayashi Terutake , Ishii-Teshima Miho , Nagai Moeto
Published: May 2020

Atomic force microscopy (AFM) is an effective platform for in vitro manipulation and analysis of living cells in medical and biological sciences. To introduce additional new features and functionalities into a conventional AFM system, we investigated the photocatalytic nanofabrication and intracellular Raman imaging of living cells by employing functionalized AFM probes. Herein, we investigated the effect of indentation speed on the cell membrane perforation of living HeLa cells based on highly localized photochemical oxidation with a catalytic titanium dioxide (TiO2)-functionalized AFM probe. On the basis of force–distance curves obtained during the indentation process, the probability of cell membrane perforation, penetration force, and cell viability was determined quantitatively. Moreover, we explored the possibility of intracellular tip-enhanced Raman spectroscopy (TERS) imaging of molecular dynamics in living cells via an AFM probe functionalized with silver nanoparticles in a homemade Raman system integrated with an inverted microscope. We successfully demonstrated that the intracellular TERS imaging has the potential to visualize distinctly different features in Raman spectra between the nucleus and the cytoplasm of a single living cell and to analyze the dynamic behavior of biomolecules inside a living cell.
Genotypic Analysis of Piroplasms and Associated Pathogens from Ticks Infesting Cattle in Korea
Authors: Seo Min-Goo , Kwon Oh-Deog , Kwak Dongmi
Published: May 2020

Tick-borne pathogens (TBPs) impose an important limitation to livestock production worldwide, especially in subtropical and tropical areas. Earlier studies in Korea have examined TBPs residing in ticks and animals; however, information on multiple TBPs in ticks infesting cattle is lacking. This study assessed the prevalence of TBPs in ticks parasitizing cattle. A total of 576 ticks, including 340 adults and 236 nymphs, were collected from cattle in Korea between 2014 and 2018. All ticks collected were identified as Haemaphysalis longicornis based on their morphological and molecular characteristics. Among piroplasms and other tick-associated pathogens, seven TBP genes, namely Theileria orientalis (5.0%), Anaplasma bovis (2.3%), Anaplasma capra (4.7%), Anaplasma phagocytophilum-like Anaplasma spp. (APL) clades A (1.9%) and B (0.5%), Ehrlichia canis (1.6%), and Candidatus Rickettsia longicornii (17.5%), were detected. Bartonella spp. and severe fever with thrombocytopenia syndrome virus were not found. To our knowledge, this is the first study to report the presence of the pathogens T. orientalis major piroplasm surface protein genotypes 3 and 7, A. capra, and APL in ticks from Korea. Cattle ticks may be maintenance hosts for many TBPs, and veterinary and medical clinicians should be aware of their high probability of infection and clinical complexity in humans.
Epstein-Barr Virus Exploits the Secretory Pathway to Release Virions
Authors: Nanbo Asuka
Published: May 2020

Herpesvirus egress mechanisms are strongly associated with intracellular compartment remodeling processes. Previously, we and other groups have described that intracellular compartments derived from the Golgi apparatus are the maturation sites of Epstein-Barr virus (EBV) virions. However, the mechanism by which these virions are released from the host cell to the extracellular milieu is poorly understood. Here, I adapted two independent induction systems of the EBV lytic cycle in vitro, in the context of Rab GTPase silencing, to characterize the EBV release pathway. Immunofluorescence staining revealed that p350/220, the major EBV glycoprotein, partially co-localized with three Rab GTPases: Rab8a, Rab10, and Rab11a. Furthermore, the knockdown of these Rab GTPases promoted the intracellular accumulation of viral structural proteins by inhibiting its distribution to the plasma membrane. Finally, the knockdown of the Rab8a, Rab10, and Rab11a proteins suppressed the release of EBV infectious virions. Taken together, these findings support the hypothesis that mature EBV virions are released from infected cells to the extracellular milieu via the secretory pathway, as well as providing new insights into the EBV life cycle.
Assessment of ISO Method 15216 to Quantify Hepatitis E Virus in Bottled Water
Authors: Cuevas-Ferrando Enric , Martínez-Murcia Antonio , Pérez-Cataluña Alba , Sánchez Gloria , Randazzo Walter
Published: May 2020

Hepatitis E virus (HEV) is one of the causative agents of water-borne human viral hepatitis and considered in Europe an emerging zoonotic pathogen. Analysis of bottled water through a standard method validated for HEV can contribute towards the risk management of this hazard. Putting some recent reports by the European Food Safety Authority in place, this study aimed to assess the performance of the concentration and extraction procedures described in ISO 15216-1:2017 for norovirus and hepatitis A virus on HEV detection. Following the ISO recommendation, the bottled water samples were spiked using serially diluted HEV fecal suspensions together with mengovirus as process control and concentrated by filtration via positively charged nylon membranes. In order to extract viral RNA from the resulting concentrates, two different methods were compared in this study: The one recommended in the ISO norm, NucliSens® MiniMag® system (NS), and an alternative commercially available kit NucleoSpin®RNA virus kit (MN). Finally, three reverse transcription quantitative PCR (RT-qPCR) assays were used to quantify HEV titers. The evaluated procedures resulted in average HEV recoveries of 14.08 ± 4.90% and 3.58 ± 0.30% for the MN and NS methods, respectively. The limit of detection (LoD95%) was 1.25 × 104 IU/L for both extraction methods combined with the three RT-qPCR assays tested, with the exception of NS extraction coupled with RT-qPCR1 that showed a LoD95% of 4.26 × 103 IU/L. The method characteristics generated in this study support the limited suitability of the ISO 15216-1:2017 concentration procedure coupled with the evaluated RT-qPCR assays for detecting HEV in bottled water.
Nasal Microbiota in RSV Bronchiolitis
Authors: Schippa Serena , Frassanito Antonella , Marazzato Massimiliano , Nenna Raffaella , Petrarca Laura , Neroni Bruna , Bonfiglio Giulia , Guerrieri Francesca , Frasca Federica , Oliveto Giuseppe , Pierangeli Alessandra , Midulla Fabio
Published: May 2020

Respiratory Syncytial Virus (RSV) is the leading cause of bronchiolitis, and the severity may be influenced by the bacterial ecosystem. Our aim was to analyze the nasal microbiota from 48 infants affected by bronchiolitis from RSV virus and 28 infants with bronchiolitis but negative for the virus. Results showed a significantly lower biodiversity in the RSV-positive group with respect to the RSV-negative group, a specific microbial profile associated with the RSV-positive group different from that observed in the negative group, and significant modifications in the relative abundance of taxa in the RSV-positive group, as well as in the RSV-A group, with respect to the negative group. Furthermore, microbial network analyses evidenced, in all studied groups, the presence of two predominant sub-networks characterized by peculiar inter- and intra-group correlation patterns as well as a general loss of connectivity among microbes in the RSV-positive group, particularly in the RSV-A group. Our results indicated that infants with more severe bronchiolitis disease, caused by RSV-A infection, present significant perturbations of both the nasal microbiota structure and the microbial relationships. Patients with a milder bronchiolitis course (RSV-B-infected and patients who have cleared the virus) presented less severe alterations.