bluuebunny/crossref_metadata_2025 huggingface dataset:
An integrated proteomic and transcriptomic signature of the failing right ventricle in monocrotaline induced pulmonary arterial hypertension in male rats
Authors: Hindmarch Charles Colin Thomas , Tian Lian , Xiong Ping Yu , Potus Francois , Bentley Rachel Emily Teresa , Al-Qazazi Ruaa , Prins Kurt W. , Archer Stephen L.
Published: November 2022

Aim: Pulmonary arterial hypertension (PAH) is an obstructive pulmonary vasculopathy that results in death from right ventricular failure (RVF). There is limited understanding of the molecular mechanisms of RVF in PAH.

Methods: In a PAH-RVF model induced by injection of adult male rats with monocrotaline (MCT; 60 mg/kg), we performed mass spectrometry to identify proteins that change in the RV as a consequence of PAH induced RVF. Bioinformatic analysis was used to integrate our previously published RNA sequencing data from an independent cohort of PAH rats.

Results: We identified 1,277 differentially regulated proteins in the RV of MCT rats compared to controls. Integration of MCT RV transcriptome and proteome data sets identified 410 targets that are concordantly regulated at the mRNA and protein levels. Functional analysis of these data revealed enriched functions, including mitochondrial metabolism, cellular respiration, and purine metabolism. We also prioritized 15 highly enriched protein:transcript pairs and confirmed their biological plausibility as contributors to RVF. We demonstrated an overlap of these differentially expressed pairs with data published by independent investigators using multiple PAH models, including the male SU5416-hypoxia model and several male rat strains.

Conclusion: Multiomic integration provides a novel view of the molecular phenotype of RVF in PAH which includes dysregulation of pathways involving purine metabolism, mitochondrial function, inflammation, and fibrosis.
Biological impact of restrictive and liberal fluid strategies at low and high PEEP levels on lung and distal organs in experimental acute respiratory distress syndrome
Authors: Felix Nathane S. , Maia Ligia A. , Rocha Nazareth N. , Rodrigues Gisele C. , Medeiros Mayck , da Silva Leticia A. , Baldavira Camila M. , Fernezlian Sandra de M. , Eher Esmeralda M. , Capelozzi Vera L. , Malbrain Manu L. N. G. , Pelosi Paolo , Rocco Patricia R. M. , Silva Pedro L.
Published: November 2022

Background: Fluid regimens in acute respiratory distress syndrome (ARDS) are conflicting. The amount of fluid and positive end-expiratory pressure (PEEP) level may interact leading to ventilator-induced lung injury (VILI). We therefore evaluated restrictive and liberal fluid strategies associated with low and high PEEP levels with regard to lung and kidney damage, as well as cardiorespiratory function in endotoxin-induced ARDS.

Methods: Thirty male Wistar rats received an intratracheal instillation of Escherichia coli lipopolysaccharide. After 24 h, the animals were anesthetized, protectively ventilated (VT = 6 ml/kg), and randomized to restrictive (5 ml/kg/h) or liberal (40 ml/kg/h) fluid strategies (Ringer lactate). Both groups were then ventilated with PEEP = 3 cmH2O (PEEP3) and PEEP = 9 cmH2O (PEEP9) for 1 h (n = 6/group). Echocardiography, arterial blood gases, and lung mechanics were evaluated throughout the experiments. Histologic analyses were done on the lungs, and molecular biology was assessed in lungs and kidneys using six non-ventilated animals with no fluid therapy.

Results: In lungs, the liberal group showed increased transpulmonary plateau pressure compared with the restrictive group (liberal, 23.5 ± 2.9 cmH2O; restrictive, 18.8 ± 2.3 cmH2O, p = 0.046) under PEEP = 9 cmH2O. Gene expression associated with inflammation (interleukin [IL]-6) was higher in the liberal-PEEP9 group than the liberal-PEEP3 group (p = 0.006) and restrictive-PEEP9 (p = 0.012), Regardless of the fluid strategy, lung mechanical power and the heterogeneity index were higher, whereas birefringence for claudin-4 and zonula-ocludens-1 gene expression were lower in the PEEP9 groups. Perivascular edema was higher in liberal groups, regardless of PEEP levels. Markers related to damage to epithelial cells [club cell secreted protein (CC16)] and the extracellular matrix (syndecan) were higher in the liberal-PEEP9 group than the liberal-PEEP3 group (p = 0.010 and p = 0.024, respectively). In kidneys, the expression of IL-6 and neutrophil gelatinase-associated lipocalin was higher in PEEP9 groups, regardless of the fluid strategy. For the liberal strategy, PEEP = 9 cmH2O compared with PEEP = 3 cmH2O reduced the right ventricle systolic volume (37%) and inferior vena cava collapsibility index (45%).

Conclusion: The combination of a liberal fluid strategy and high PEEP led to more lung damage. The application of high PEEP, regardless of the fluid strategy, may also be deleterious to kidneys.
Niche divergence at the intraspecific level in an endemic rare peony (Paeonia rockii): A phylogenetic, climatic and environmental survey
Authors: Dong Peng-Bin , Wang Ling-Juan , Jia Yun , Li Zhong-Hu , Wang Hong-Yan , Guo Feng-Xia , Chen Yuan
Published: November 2022

Ecological factors have received increasing attention as drivers of speciation but also in the maintenance of postspeciation divergence. However, the relative significance of the responses of species to climate oscillations for driving niche divergence or conservatism in the evolution of many species that pass through diverse environments and limited geographical boundaries remains poorly understood. Paeonia rockii (one of the ancient species of Paeonia) comprising two subspecies called Paeonia rockii subsp. rockii and Paeonia rockii subsp. taibaishanica is an endemic, rare, and endangered medicinal plant in China. In this study, we integrated whole chloroplast genomes, and ecological factors to obtain insights into ecological speciation and species divergence in this endemic rare peony. RAxML analysis indicated that the topological trees recovered from three different data sets were identical, where P. rockii subsp. rockii and P. rockii subsp. taibaishanica clustered together, and molecular dating analyses suggested that the two subspecies diverged 0.83 million years ago. In addition, ecological niche modeling showed that the predicted suitable distribution areas for P. rockii subsp. rockii and P. rockii subsp. taibaishanica differed considerably, although the predicted core distribution areas were similar, where the population contracted in the last interglacial and expanded in the last glacial maximum. Under the emissions scenarios for the 2050s and 2070s, the suitable distribution areas were predicted to contract significantly, where the migration routes of the two subspecies tended to migrate toward high latitudes and elevations, thereby suggesting strong responses of the distributions of the two subspecies to climate change. These findings combined with the phylogeographic relationships provide comprehensive insights into niche variation and differentiation in this endemic rare peony, and they highlight the importance of geological and climatic changes for species divergence and changes in the population geographic patterns of rare and endangered medicinal plants in East Asia.
Nitric oxide: A core signaling molecule under elevated GHGs (CO2, CH4, N2O, O3)-mediated abiotic stress in plants
Authors: Kabange Nkulu Rolly , Mun Bong-Gyu , Lee So-Myeong , Kwon Youngho , Lee Dasol , Lee Geun-Mo , Yun Byung-Wook , Lee Jong-Hee
Published: November 2022

Nitric oxide (NO), an ancient molecule with multiple roles in plants, has gained momentum and continues to govern plant biosciences-related research. NO, known to be involved in diverse physiological and biological processes, is a central molecule mediating cellular redox homeostasis under abiotic and biotic stresses. NO signaling interacts with various signaling networks to govern the adaptive response mechanism towards stress tolerance. Although diverging views question the role of plants in the current greenhouse gases (GHGs) budget, it is widely accepted that plants contribute, in one way or another, to the release of GHGs (carbon dioxide (CO2), methane (CH4), nitrous oxide (N2O) and ozone (O3)) to the atmosphere, with CH4and N2O being the most abundant, and occur simultaneously. Studies support that elevated concentrations of GHGs trigger similar signaling pathways to that observed in commonly studied abiotic stresses. In the process, NO plays a forefront role, in which the nitrogen metabolism is tightly related. Regardless of their beneficial roles in plants at a certain level of accumulation, high concentrations of CO2, CH4, and N2O-mediating stress in plants exacerbate the production of reactive oxygen (ROS) and nitrogen (RNS) species. This review assesses and discusses the current knowledge of NO signaling and its interaction with other signaling pathways, here focusing on the reported calcium (Ca2+) and hormonal signaling, under elevated GHGs along with the associated mechanisms underlying GHGs-induced stress in plants.
Post-error behavioral adjustments under reactive control among older adults
Authors: Tsuchida Noriaki , Kasuga Ayaka , Kawakami Miki
Published: November 2022

This study analyzed the effects of aging on post-error behavioral adjustments from the perspective of cognitive control. A modified error awareness task was administered to young (n = 50) and older (n = 50) adults. In this task, two buttons were placed on the left and right sides in front of the participants, who were instructed to use the right button to perform a go/no-go task, and were notified if they made an error. There were three experimental conditions (A, B, and C): participants had to push the right button once in Condition A and twice in Condition B and C when a go-stimulus was presented. Conversely, participants were asked to withhold their response when a no-go stimulus was presented. Response inhibition differed depending on the experimental condition. The participants were asked to push the left button as quickly as possible when an error occurred. The results showed relatively longer reaction times to sudden errors among older adults compared with young adults. Furthermore, the difference in the error responses (i.e., accidentally pushing the right button once or twice when a no-go stimulus was presented) strongly influenced older adults’ response time after an error. These results suggest that the shift from proactive to reactive control may significantly influence post-error behavioral adjustments in older adults.
Increased perceived autonomy-supportive teaching in physical education classes changes students’ positive emotional perception compared to controlling teaching
Authors: Leisterer Sascha , Paschold Elias
Published: November 2022

Teachers can expect that autonomy support positively influences students’ affective-emotional perception in physical education (PE), when considering assumptions of the Self-Determination theory. Highly autonomy-supportive PE teaching comprises students’ free choices regarding organizational, procedural, and cognitive aspects of a PE lesson, whereas low autonomy support addresses these aspects only partly and controlling teaching refers to students as recipients of the teacher’s decisions. This quasi-experiment investigates effects to determine the effects of high autonomy-supportive (PEhigh), low autonomy-supportive (PElow) and controlling (PEcontrol) PE class teaching styles on affective valence and enjoyment. As such, we compare the effects of these teaching styles on beneficial psychological outcomes (i.e., affective valence, enjoyment) in students. In a sample of German students (N = 57; age: M ± SD = 15.6 ± 0.6; gender: 53% female, 47% male) perceived autonomy support, affective valence, and enjoyment were assessed via self-report questionnaires before and after a 20-min PE class intervention focusing on high or low autonomy-supportive, or controlling teaching. Students who participated in PEhigh perceived significantly more positive valence and enjoyment over time compared to students in the PElow and PEcontrol groups (affective valence: p = 0.025, ηp2 = 0.13; enjoyment: p = 0.007, ηp2 = 0.17). Differences between groups show significant results for valence between PEhigh and PEcontrol, and between PElow and PEcontrol. Thus, PEhigh should be preferred over PElow to intensify these effects. Based on these results, PE teachers can employ a high autonomy-supportive teaching style (e.g., through a combination of free choices, social interaction, and informative feedback) to improve students’ positive affective-emotional perception and to foster an increase in students’ time engaged in physical activity.
Linking the trust of industrial entrepreneurs on elements of ecosystem with entrepreneurial success: Determining startup behavior as mediator and entrepreneurial strategy as moderator
Authors: Rehman Zia Ur , Arif Muhammad , Gul Habib , Raza Jamshed
Published: November 2022

Purpose

This study aimed to apply “multi-criteria decision approach and attitude-change theory” to examine post-COVID-19 impact on entrepreneurial mindset by investigating the link between entrepreneurs social capital (trust on three elements of ecosystem i.e., experts & enterprises, media, and government) and entrepreneurial success (both individual and organizational). Specifically, this study analyzed entrepreneurs' dispositional factor (startup behavior) as an underlying mechanism to bridge trust and entrepreneurial success. Furthermore, it also analyzed entrepreneurs' situational factor (entrepreneurial strategy) as boundary condition.

Design/methodology/approach

We applied time-lagged data collection from 505 industrial entrepreneurs. Survey method was used for data collection. A 7-point Likert scale was used for the respondent response. Hayes developed PROCESS models 4 and 7 were used to test the hypothesis.

Findings

The direct impact of trust on three elements of the ecosystem was found significantly positive on both startup behavior and entrepreneurial success. The direct impact of startup behavior on entrepreneurial success is also significantly positive. The impact of startup behavior on indirect mediation between trust and entrepreneurial success is visibly positive. The moderated and moderated mediation impact of entrepreneurial strategy found positively significant at low and medium values. However, this study found an insignificant moderated impact at high values of entrepreneurial strategy between trust on media and startup behavior. Furthermore, this study also found insignificant moderated mediation impact at high values of entrepreneurial strategy by interacting with two elements of ecosystem (trust on media and trust on government) through startup behavior on entrepreneurial success.

Originality/value

The authors suggested that startup behavior is an underlying mechanism through which industrial entrepreneurs trust achieved desired entrepreneurial success. The authors also suggested that the influencing role of “low level of entrepreneurial strategy” in comparison with “high level entrepreneurial strategy” is more helpful to achieve entrepreneurial success.

Implications

This study contributed to the literature on entrepreneurial strategy for its conditional indirect moderated impact on startup behavior and moderated mediation impact on firm entrepreneurial success. It also contributed to owners of the manufacturing industry for their startup behavior as an underlying mechanism through which trust influences entrepreneurial success.
An analytical study of employee loyalty and corporate culture satisfaction assessment based on sentiment analysis
Authors: Xie Jie , Su Ri Le Ge , Song Jaehoon
Published: November 2022

As an important factor related to the interests of enterprises, the attitude and behavior of employees are related to the company’s survival and the realization of business objectives. However, in recent years, with the rapid development of emerging industries and industry changes, the turnover rate of employees in the whole enterprise has been greatly improved. Frequent turnover of personnel will have a great impact on the stability of the company, the competitiveness of the enterprise, and the operating cost. Employee loyalty and corporate culture satisfaction assessment is the core of sustainable corporate development, but most of the traditional analysis studies are based on the performance approach, using employee overtime, employee achievement, and company effectiveness as data sources, the former performance data is only a side view of the company’s situation, the latter as the data source of this study is a one-sided and involute form, and the data is not stable. Therefore, this paper proposes an analysis of employee loyalty and corporate culture satisfaction assessment based on sentiment analysis, using association rules and sentiment lexicons from anonymous employee evaluations to analyze the actual data.
Associations between adherence to the Taiwan Daily Food Guide and psychiatric morbidity: A population-based study in Taiwan
Authors: Li Ming-Chieh
Published: November 2022

Background

Mental health has become a public health concern worldwide, and the number of affected individuals is rising. Therefore, further research must be conducted to identify potential risk factors to develop optimal prevention strategies to mitigate mental health disorders.

Methods

Using Taiwanese Nutrition and Health Survey data collected from 2013–2016, we conducted a cross-sectional study to examine whether adherence to the Taiwan Daily Food Guide affects mental health conditions. Study participants were adults aged ≥19 years. The dietary assessment was conducted using a validated food frequency questionnaire. The presence of psychiatric morbidity was defined as a five-item Brief Symptom Rating Scale (BSRS-5) score of ≥10. Logistic regression models were used to determine whether Taiwan Daily Food Guide adherence was related to the presence of psychiatric morbidity.

Results

After adjusting for potential confounders, we observed protective associations between adherence to the Taiwan Daily Food Guide and psychiatric morbidity risk.

Conclusion

The Taiwan Daily Food Guide might reduce the risk associated with psychiatric morbidity and could be a reference for developing a national food guide for mental health.
Assessment of mental wellbeing of undergraduate pharmacy students from 14 countries: The role of gender, lifestyle, health-related, and academic-related factors
Authors: Elnaem Mohamed Hassan , Mubarak Naeem , K. T. Mohammed Salim , Barakat Muna , Abdelaziz Doaa H. , Mansour Noha O. , Thabit Abrar K. , Ramatillah Diana Laila , Al-Jumaili Ali Azeez , Syed Nabeel Kashan , Adam Mohammed Fathelrahman , Hossain Md. Sanower , Baraka Mohamed A. , Jose Jimmy , Elkalmi Ramadan , Chandran Sarath , Singh Dehele Inderpal , Elrggal Mahmoud , Fathelrahman Ahmed Ibrahim
Published: November 2022

Background

Pharmacy students will assume future roles as frontline healthcare providers. Therefore, evaluating their current state of mental wellbeing and its associated factors is essential for better planning students' support initiatives. This study aimed to assess mental wellbeing and its associated factors among undergraduate pharmacy students from 14 countries during the pandemic.

Methods

A cross-sectional study was conducted among undergraduate pharmacy students in 14 countries in Asia and the Middle East. The validated Warwick-Edinburgh Mental Wellbeing Scale (the 14-item WEMWBS) was adopted to assess mental wellbeing. Data collection was performed online between February and April 2022. Descriptive and inferential statistics were used as appropriate.

Results

A total of 2,665 responses were received, mainly from females (68.7%) with a higher presence of private universities (59.1%). About 34.9% had low mental wellbeing levels, while 57 and 8.1% had medium, and high levels, respectively. Binary logistic regression showed that males (AOR: 1.34; CI 95%: 1.11–1.61; p < 0.01) and students with no chronic illnesses (AOR: 2.01; CI 95%: 1.45–2.80; p < 0.001) were more likely to have higher mental wellbeing. Also, participants who did not engage in any exercise (AOR: 0.71; CI 95%: 0.52–0.98; p = 0.04) and those in public universities (AOR: 0.82; CI 95%: 0.69–0.97; p = 0.02) were less likely to have higher mental wellbeing. Additionally, students who had interest/passion for pharmacy (AOR: 1.69; CI 95%: 1.07–2.68; p = 0.02), and those who known pharmacists inspired (AOR: 1.81; CI 95%: 1.06–3.12; p = 0.03), were more likely to have higher mental wellbeing compared with those who had no specific reason for their choice to study pharmacy. The participants with excellent (AOR: 1.87; CI 95%: 1.29–2.70; p = 0.001) or very good self-reported academic performance (AOR: 1.57; CI 95%: 1.12–2.22; p = 0.01) were more likely to have higher mental wellbeing compared to those with fair academic performance.

Conclusion

More than a third of the participants had low mental wellbeing. Various demographic, lifestyle, medical and academic factors appeared to affect students' mental wellbeing. Careful consideration of these factors and their integration into the pharmacy schools' plans for student support services and academic advising would be essential to improve students' mental wellbeing.
A comparative study of the efficacy and functionality of 10 commercially available wrist-hand orthoses in healthy females during activities of daily living
Authors: Aranceta-Garza Alejandra , Ross Karyn
Published: November 2022

Objective

Optimal wrist/hand function facilitates the performance of activities of daily living (ADL), which are associated with independent living and increased quality of life. Rheumatological, musculoskeletal, and neurological conditions or injuries can negatively impact hand/wrist function, with wrist-hand orthoses (WHOs) being prescribed to control motion and improve wrist alignment whilst enhancing hand/wrist functionality. The objective of this follow-up study was to quantify and assess the efficacy and functionality of 10 commercially available WHOs during five ADLs.

Design

Randomised comparative functional study of the wrist/hand with and without WHOs.

Participants

Ten right-handed healthy female participants with no underlying condition or pain affecting the wrist/hand that could influence their ability to undertake ADLs.

Main outcome measures

The primary outcome was ascertaining the impact of each WHO during five ADLs. Movement was quantified in sagittal, coronal, and transverse planes with and without WHO use. The resting position, maximum mean flexion, extension, pronation, supination, and radial and ulnar deviation attained were quantified, with the time spent in wrist flexion, wrist flexion and ulnar deviation, wrist extension >15°, and radial deviation recorded. Finally, the time to complete each task was compared between conditions.

Results

At rest, four WHOs maintained the desired sagittal plane wrist position, with only one preventing radial deviation with variation observed in the transverse plane. All WHOs reduced mean maximum flexion, with only 10 out of 50 tests (20%) showing a successful restriction of flexion (p < 0.05) and 14 out of 50 (28%) showing a reduction of the time spent in flexion (p < 0.05). In 42 out of 50 tests (84%), the wrist was extended >15° for a significant amount of time (p < 0.05), with the wrist in radial deviation in 98% for a significant amount of time (p < 0.001). The wrist was flexed and in ulnar deviation for a significant time for 6 out of 50 tests (12%, p < 0.05), whilst all WHOs impacted transverse movement, with 27% reducing it significantly, and all tasks took a longer time to complete, with 46% taking a significantly longer time (p < 0.05).

Conclusion

The WHOs did not control movement sufficiently to successfully manage any condition requiring motion restriction associated with pain relief and were found to increase the time to complete the ADLs. Multifactorial design aspects influenced functionality, and there is a clear need for WHO redesign.
Tracking dynamic changes in implementation strategies over time within a hybrid type 2 trial of an electronic patient-reported oncology symptom and needs monitoring program
Authors: Smith Justin D. , Merle James L. , Webster Kimberly A. , Cahue September , Penedo Frank J. , Garcia Sofia F.
Published: November 2022

Background

Longitudinal tracking of implementation strategies is critical in accurately reporting when and why they are used, for promoting rigor and reproducibility in implementation research, and could facilitate generalizable knowledge if similar methods are used across research projects. This article focuses on tracking dynamic changes in the use of implementation strategies over time within a hybrid type 2 effectiveness-implementation trial of an evidence-based electronic patient-reported oncology symptom assessment for cancer patient-reported outcomes in a single large healthcare system.

Methods

The Longitudinal Implementation Strategies Tracking System (LISTS), a timeline follow-back procedure for documenting strategy use and modifications, was applied to the multiyear study. The research team used observation, study records, and reports from implementers to complete LISTS in an electronic data entry system. Types of modifications and reasons were categorized. Determinants associated with each strategy were collected as a justification for strategy use and a potential explanation for strategy modifications.

Results

Thirty-four discrete implementation strategies were used and at least one strategy was used from each of the nine strategy categories from the Expert Recommendations for Implementing Change (ERIC) taxonomy. Most of the strategies were introduced, used, and continued or discontinued according to a prospective implementation plan. Relatedly, a small number of strategies were introduced, the majority unplanned, because of the changing healthcare landscape, or to address an emergent barrier. Despite changing implementation context, there were relatively few modifications to the way strategies were enacted, such as a change in the actor, action, or dose. Few differences were noted between the trial's three regional units under investigation.

Conclusion

This study occurred within the ambulatory oncology clinics of a large, academic medical center and was supported by the Quality team of the health system to ensure greater uptake, uniformity, and implementation within established practice change processes. The centralized nature of the implementation likely contributed to the relatively low proportion of modified strategies and the high degree of uniformity across regions. These results demonstrate the potential of LISTS in gathering the level of data needed to understand the impact of the many implementation strategies used to support adoption and delivery of a multilevel innovation.

Clinical trial registration

https://clinicaltrials.gov/ct2/show/NCT04014751, identifier: NCT04014751.
Growth, maturation and injuries in high-level youth football (soccer): A mini review
Authors: Wik Eirik Halvorsen
Published: November 2022

Understanding the challenges football (soccer) players face during adolescence is fundamental to avoid disruptions in their development due to injury. This mini review will describe basic concepts of somatic growth and biological maturity, examine data from 53 prospective epidemiological studies on high-level youth football players and discuss how age, growth and maturity may affect the injury patterns observed. Based on the existing evidence, at least every third player sustains an injury during a football season. The thigh (median for studies of boys: 25%, median for girls: 21%), ankle (b: 18%, g: 30%), knee (b: 17%, g: 18%) and hip/groin (b: 14%, g: 10%) are the body parts injured most often, while muscle strains (b: 31%, g: 25%), sprains (b: 20%, g: 27%) and contusions (b: 17%, g: 16%) are the most common injury types. Injury trends are, however, not consistent throughout adolescence, and players' age, maturity status and position relative to peak height velocity (PHV) have shown to influence the number, type and location of injuries sustained. Despite a high volume of observational injury studies published on high-level youth players, girls (7 studies) and settings outside of Europe (included in 23% of studies) are underrepresented and should receive extra attention in the future. Based on the available epidemiological data, tailored injury reduction programmes can be considered in youth football, alongside application of general training principles such as progression, variation and individualization which may be especially important during vulnerable phases such as the adolescent growth spurt.
Comparison of laparoscopic and open approach in the treatment of heterotopic pregnancy following embryo transfer
Authors: Chen Shengfu , Zhu Yingying , Xie Meiqing
Published: November 2022

Purpose

Heterotopic pregnancy (HP) is a rare disease with the coexistence of an intrauterine and ectopic embryos. There is no consensus on the optimal treatment of HP at present. This research aimed to compare the perioperative and pregnancy outcomes of laparoscopic (LA) and open approach (OA) in patients with HP after embryo transfer.

Methods

Women with HP receiving surgical treatment (LA or OA) were retrospectively recruited in this study between October 2006 and December 2020. The demographic, perioperative and obstetric data were collected and compared between LA and OA group.

Results

Totally, 86 patients were included in this study. Among these patients, 62 underwent LA and 24 underwent OA. There was an increase in the adoption of LA between the 2006–2012 period and the 2013–2020 period [25% (6/24) vs. 90% (56/62), p < 0.001]. Compared with OA, patients treated by LA had much less blood loss [20 (10–50) vs. 30 (20–50) ml, p = 0.036] and fewer days of hospital stay [5.0 (4.0–7.3) vs. 9.5 (7–15.3) days, p < 0.001], but a relatively higher cost (15,580 ± 3196¥ vs. 11,717 ± 3820¥, p < 0.001). During the laparoscopic procedure, no one needed to be converted to open surgery. However, the rates of first trimester miscarriage, preterm, cesarean section, birth weight, 1- and 5-min Apgar were similar between LA and OA group (all p > 0.05).

Conclusions

Compared with open approach, laparoscopy was shown to provide a comparable pregnancy outcomes and a better performance on perioperative outcomes in the treatment of HP patients with embryo transfer.
Oxidative stress-related patterns determination for establishment of prognostic models, and characteristics of tumor microenvironment infiltration
Authors: Bai Zihao , Bai Yihua , Fang Changzhong , Chen Wenliang
Published: November 2022

Oxidative stress-mediated excessive accumulation of ROS in the body destroys cell homeostasis and participates in various diseases. However, the relationship between oxidative stress-related genes (ORGs) and tumor microenvironment (TME) in gastric cancer remains poorly understood. For improving the treatment strategy of GC, it is necessary to explore the relationship among them. We describe the changes of ORGs in 732 gastric cancer samples from two data sets. The two different molecular subtypes revealed that the changes of ORGs were associated with clinical features, prognosis, and TME. Subsequently, the OE_score was related to RFS, as confirmed by the correlation between OE_score and TME, TMB, MSI, immunotherapy, stem cell analysis, chemotherapeutic drugs, etc. OE_score can be used as an independent predictive marker for the treatment and prognosis of gastric cancer. Further, a Norman diagram was established to improve clinical practicability. Our research showed a potential role of ORGs in clinical features, prognosis, and tumor microenvironment of gastric cancer. Our research findings broaden the understanding of gastric cancer ORGs as a potential target for individualized treatment of gastric cancer and a new direction to evaluate the prognosis.
Predictive value of S100A4 in eosinophilic chronic rhinosinusitis with nasal polyps
Authors: Zou Shangchu , Huang Zhicheng , Wu Jinpeng
Published: November 2022

Objective

S100A4 is a pro-inflammatory mediator which has been implicated in airway inflammatory diseases. However, its role in chronic rhinosinusitis with nasal polyps (CRSwNP) remains unclear. The purpose of this study is to determine the expression of S100A4 and evaluate its potential value in distinguishing its endotypes.

Methods

Sixty CRSwNP patients, 30 chronic rhinosinusitis without nasal polyps (CRSsNP) patients, and 30 healthy controls (HC) were enrolled in this study, and serum and tissue samples were collected. Serum and tissue S100A4 levels were detected by enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, western blotting and immunofluorescence. Their clinical values in predicting postoperative recurrence of CRSwNP were evaluated by multivariate analysis and ROC curves.

Results

Serum levels of S100A4 were notably increased in the CRSwNP group than in the CRSsNP and HC groups (p < 0.05), and positively correlated with tissue and peripheral eosinophil count and percentage (p < 0.05). The serum S100A4 concentrations were significantly elevated in the Eos CRSwNP group in comparison with the non-Eos CRSwNP group (p < 0.05). Multivariate analysis and ROC curve presented that serum S100A4 levels were associated with CRSwNP endotypes. Additionally, tissue S100A4 mRNA and protein levels were significantly enhanced in the CRSwNP group than in the HC group and CRSsNP group, especially in the Eos CRSwNP group.

Conclusion

Our results demonstrated that the S100A4 expression was increased in CRSwNP patients and associated with the endotypes. S100A4 could be a serologic biomarker for evaluating tissue eosinophilic inflammation and predicting endotypes in CRSwNP patients.
Terminación Prematura en Psicoterapia y Características de los Pacientes: un estudio empírico
Authors: Santangelo Pablo Rafael , Conde Karina , Mattiello Marcos Emanuel
Published: November 2022

Considerar la terminación prematura en psicoterapia es esencial cuando hablamos de eficacia y efectividad. El objetivo de este trabajo fue evaluar la relación entre las características demográficas de los pacientes y el nivel de severidad al inicio del tratamiento con la terminación prematura en psicoterapia. Método. Estudio longitudinal, con una muestra clínica de N = 134 pacientes. Se administraron en el proceso de admisión el PID – 5 y el OQ.45.2 y en la tercera sesión de psicoterapia, octava y última sesión el OQ.45.2.  Se realizaron análisis de regresión logística con deserción como variable de resultado, y correlaciones de Spearman para estimar la relación entre las características personales y la cantidad de sesiones en el grupo que abandonó el proceso psicoterapéutico. Resultados. El malestar al inicio del proceso psicoterapéutico no se relacionó con la deserción. La única faceta de personalidad relacionada con la deserción fue Labilidad Emocional, a menor Labilidad Emocional, hubo una probabilidad 16% menor de deserción. Un mayor nivel socioeconómico y nivel de instrucción redujeron en un 42% y 16% las probabilidades de deserción, respectivamente. Los varones, por su parte, tuvieron 3 veces más probabilidad de abandonar el tratamiento que las mujeres.
La formulación del caso desde la perspectiva de una mente gobernada por objetivos y creencias
Authors: Saliani Angelo Maria , Astiaso Garcia Benedetto , Perdighe Claudia , Barcaccia Barbara , Mancini Francesco
Published: November 2022

La formulación del caso (FC) es el requisito necesario de cada tratamiento psicoterapéutico,independientemente de la orientación teórica del clínico. Sin una FC no hay un mapa delfuncionamiento del problema que aflige al paciente y, en consecuencia, no hay posibilidad de unaestrategia terapéutica clara derivada de ella. En este artículo se presenta un método de FC en cinco puntos adaptado a la singularidad delpaciente que tenga en consideración la descripción del problema, su posible clasificacióndiagnóstica y, sobre todo, su explicación. La explicación se refiere a las causas psicológicaspróximas y remotas del problema concreto, las que determinan su mantenimiento y, por último, lasprecipitantes, es decir, las que provocaron su aparición. Llamamos a los cinco puntos de la FC: 1)descripción del problema y diagnóstico clínico; 2) perfil interno; 3) mantenimiento; 4)descompensación; 5) vulnerabilidad.

El marco teórico en el que se inscribe este tipo de FC es el de una psicoterapia cognitiva (TC)inspirada en un modelo finalista de la mente (Miller, Galanter, Pribram 1960), en el que, en laexplicación del comportamiento individual, junto a las creencias, juegan un papel fundamental losobjetivos sobrevalorados por el individuo.Por último, se discutirán las importantes implicaciones de la estrategia terapéutica que involucraneste tipo de formulación y la necesidad de conocer y aplicar los principios que rigen los procesos deaceptación cognitiva.
Innovative Moments and Case Formulation
Authors: Magalhães Carina , Pinheiro Patrícia , Gonçalves Miguel , Silva Jéssica
Published: November 2022

At the beginning of the therapeutic process, clients frequently feel immersed in their problems, and demoralized, with a negative perspective of different areas of their lives. The clients´ discourse is specially focused on difficulties and previous ineffective attempts to overcome their problems. Even though positive aspects of the clients’ life continue to exist, such as resources and strengths, they have little visibility in the clients’ speech and, consequently, in the therapist’s assessment and intervention planning. Moreover, most psychotherapy models emphasize a focus on the deficits of the clients, centered on the correction of difficulties as a way to achieve mental health. In this context, we highlight the importance of integrating resources and strengths of the clients’ life in case assessment, clinical formulation, and treatment planning. This information can be collected in the assessment phase, but in order to make it explicit the relevance of this information we created a protocol designed for this purpose (Feedback Initiated Narrative Development Protocol [FIND]). The first component of FIND invites clients to reflect on aspects beyond their problem like their personal strengths, significant values, positive autobiographical episodes, and useful strategies that have been adopted in the past. This interview was largely influenced by narrative therapy and innovative moments research, and it provides information regarding clients’ resources that may be useful to psychotherapists from different approaches. In this paper, we presented a clinical case formulation considering resources evaluated with the FIND protocol.
Reconstruction of Spiritual Marketing, Culture of Innovation, Quality of Work Life, and Retainers for Tourism Industry SMEs in East Java
Authors: Hermawati Adya , Anam Choirul , Suwarta Suwarta , Puspitosarie Endah
Published: November 2022

The purpose of this study is to reconstruct and develop a more comprehensive competitive advantage concept model by combining the variables of spiritual marketing, innovation culture, quality of work life, and rewards for the tourism industry’s Small and Medium-Sized Enterprises (SMEs) in East Java. This research uses a positive (quantitative) approach. A quantitative approach involves a survey method applied to a sample taken from the population. Data analysis was performed using the statistic method known as IPA (importance performance analysis). The analysis unit encompasses the employees of tourism industry SMEs in East Java Province. Among all the variables in this study, the biggest analysis gap is innovative culture. Tourism industry SMEs in East Java Province should prioritize innovative culture to help improve the performance of SMEs. The smallest analysis gap in this study is spiritual marketing. Tourism industry SMEs must at least maintain their performance. The scientific contribution of this study is its research object, which is tourism industry SMEs in East Java Province. Another scientific contribution is that this study attempts to reconstruct or develop a more comprehensive model of competitive advantage by including spiritual marketing and quality of work life as explanatory variables.
Crocus sativus (L.) Grown in Pots with High Volume Capacity: From a Case of Study to a Patent
Authors: Cicco Nunzia
Published: November 2022

Saffron (Crocus sativus L.) cultivation is widespread in different parts of the world, including various Mediterranean areas. The crop management techniques, requiring intensive manual labor from planting, weeding, flower picking to the collecting of stigmas, contribute greatly to the high price of the spice. Traditionally, the corms are cultivated in field soil and only stigmas are collected to obtain the spice while the flower’s remaining parts, corresponding to about 90% of the total biomass, are discarded and thrown away. In this study, in order to overcome some difficulties occurring during the whole crop cycle (pathogens, fungi, pests, weeds, etc.), as well as to ease and increase floral bioresidue recovery, an alternative planting way for Crocus sativus L. was proposed relying on the use of large pots. For this aim, corms with 3.0–3.5 cm diameter size, from two different geographical origins (Spain, Holland), were planted in plastic pots with a volume of 250 L or 350 L, placed in two different areas of the Basilicata region (Italy). The effect of this new growing condition on dry stigma yield as well as daughter corm yield and size was evaluated. Although this cultivation system is more expensive than the traditional one, it offers numerous and huge advantages. Among them, it allows us to maintain a more correct posture and to preserve flower integrity during harvesting. The structural integrity of the tepals is a very important factor to obtain innovative dried flowers in their original tridimensional shape (3D). Consequently, the proposed cultivation system facilitates the achievement of a real “niche product” with high added value (absence of pollen grains). Moreover, the qualitative analysis of the spice, performed according to the International Standardization Organization Normative 3632 (ISO 3632-2/1:2010/2011), classified all investigated saffron samples in the first qualitative category. The results of the first three trial years are very exciting and promising as they are similar to those from the literature carried out in ground soil. However, corms from Spain gave the best results. Further investigations are in progress in order to optimize this alternative cultivation system.
Effects of Mechanical Transplanting Methods and Planting Geometry on Yield Formation and Canopy Structure of Indica Rice under Rice-Crayfish Rotation
Authors: Li Yangyang , Dou Zhi , Guo Halun , Xu Qiang , Jiang Junliang , Che Yang , Li Jian , Liu Yaju , Gao Hui
Published: November 2022

The rice–crayfish continuous production system developed rapidly due to its high economic benefits and eco-friendly nature in China. This study explored the effects of mechanically transplanted methods and planting geometry on the relationship between rice yield and canopy structure, under rice-crayfish rotation using excellent-quality indica rice, and carried out in 2018 and 2019. Three mechanical transplantation methods were set as follows: carpet seedlings mechanically transplanted with 30 cm equal row spacing (CMTE), pot seedlings mechanically transplanted with narrow row spacing with alternating 23 cm/33 cm wide row spacing (PMTWN), and equal row spacing at 28 cm (PMTE). Different plant spacings (CMTE1-CMTE6, PMTWN1-PMTWN6, PMTE3, and PMTE4) were set in accordance with different mechanical transplanting methods. CMTE and PMTWN both included six transplanting densities, while PMTE included 2 transplanting densities. Results showed that rice yield was improved by 2.87–6.59% under PMTWN when compared to CMTE, which was mainly due to the increase in spikelets per panicle and filled-grain percentage. Dry matter accumulation was increased and larger leaf area indexes were observed under PMTWN than CMTE at the rice main growth stage. Yield of CMTE and PMTWN treatments increased at first and then declined with decreased planting density. Under suitable planting density, PMTWN could optimize rice population structure and increase rice yield compared with PMTE. For tested rice variety, pot seedlings were mechanically transplanted alternating alternating 23 cm/33 cm wide row spacing, combined with a plant spacing of 16.8 cm, was proper for its yield improvement under rice-crayfish rotation.
Effects of Extraction Solvents on the Total Phenolic Content, Total Flavonoid Content, and Antioxidant Activity in the Aerial Part of Root Vegetables
Authors: Mohammed Eman A. , Abdalla Ismat G. , Alfawaz Mohammed A. , Mohammed Mohammed A. , Al Maiman Salah A. , Osman Magdi A. , Yagoub Abu ElGasim A. , Hassan Amro B.
Published: November 2022

The present study aimed to investigate the total phenolics, total flavonoids, and antioxidant activity in terms of the DPPH scavenging, reducing power, and H2O2 scavenging of the aerial parts of onion, white radish, red radish, carrot, and beet as affected by different extraction solvents. Generally, the aerial part of these vegetables has high antioxidant properties. Samples were extracted with methanol (100 and 70%), ethanol (100 and 70%), and water. Total phenolic content was highest when the samples were extracted using 100% methanol, while extraction with 100% ethanol yielded the highest total flavonoids. The highest DPPH activity and H2O2 scavenging values were obtained by extraction of the aerial plant parts with 70% ethanol, and the 70% methanol extract had the highest reducing power. Partial least regression (PLS) was performed to validate the optimum solvent for extraction of the antioxidants and their activity in each plant. The PLS indicated that there was a variation in the validation of the different extracts for each plant. The high antioxidant capacity of root vegetables, which is natural, indicates that they may have health and dietetic advantages for consumers.
Changes in Acute Phase Response Biomarkers in Racing Endurance Horses
Authors: Mihelić Karla , Vrbanac Zoran , Bojanić Krunoslav , Kostanjšak Tara , Ljubić Blanka Beer , Gotić Jelena , Vnuk Dražen , Bottegaro Nika Brkljača
Published: November 2022

This study aimed to evaluate if exercise-induced acute phase response (APR) occurs in endurance horses in response to the race. The study included 23 horses competing in an endurance competition with a successfully passed clinical examination before the race. Blood samples were collected before the start and within 30 min after the end of the race. Haematological and biochemical tests were performed and correlated to acute phase biomarkers changes. Values of calprotectin and haptoglobin (Hp) decreased after the races compared to values before, while concentrations of ceruloplasmin and albumin recorded a significant increase. Greater changes in calprotectin values were noted in Arabian horses compared to other breeds. Values of Hp showed a significantly greater decrease after longer races. Based on study results, endurance racing induces APR in horses characterised by significant changes in selected acute phase biomarkers. More pronounced changes were noted at races with higher average speeds, suggesting the need for thorough horse monitoring during exhausting races.