bluuebunny/crossref_metadata_2025 huggingface dataset:
Incorporating Citizen Science to Enhance Public Awareness in Smart Cities: The Case Study of Balaguer
Authors: Cabeza Luisa F. , Teixidó Mercè , Guarino Francesco , Rincione Roberta , Díaz Manel , Gil Rosa M. , Cellura Maurizio , Mateu Carles
Published: March 2024

The concept of a smart city is becoming increasingly popular to improve citizens’ quality of life. Institutions are also committed to enhancing the sustainability of cities by implementing the Sustainable Development Goals (SDGs). This paper presents a Balaguer case study investigating energy demand monitoring, decreasing energy demand, and citizen acceptance in a municipality district. The study collected data from three sources: (1) quantitative data coming from on-site sensors; (2) quantitative data from a simulation of the area; and (3) qualitative data from questionnaires developed with a totem located in the city center. This study shows the importance of citizen science in contributing towards the increased awareness of energy demand, renewable energy, and climate change. But it also shows how citizen science can improve research quality involving the municipality authorities. This study also was instrumental in contributing to the increase in awareness among municipality authorities and capacity building on the topic. This activity may also contribute towards the implementation of actions to reduce the energy demand in public buildings and helping them in deploying policies to decrease energy demand in buildings, increase the use of renewable energy, and increase awareness among citizens. The government will use the information gathered to develop policies for citizen improvement.
Tayseer: A Novel AI-Powered Arabic Chatbot Framework for Technical and Vocational Student Helpdesk Services and Enhancing Student Interactions
Authors: Alabbas Abeer , Alomar Khalid
Published: March 2024

The rise of conversational agents (CAs) like chatbots in education has increased the demand for advisory services. However, student–college admission interactions remain manual and burdensome for staff. Leveraging CAs could streamline the admission process, providing efficient advisory support. Moreover, limited research has explored the role of Arabic chatbots in education. This study introduces Tayseer, an Arabic AI-powered web chatbot that enables instant access to college information and communication between students and colleges. This study aims to improve the abilities of chatbots by integrating features into one model, including responding with audiovisuals, various interaction modes (menu, text, or both), and collecting survey responses. Tayseer uses deep learning models within the RASA framework, incorporating a customized Arabic natural language processing pipeline for intent classification, entity extraction, and response retrieval. Tayseer was deployed at the Technical College for Girls in Najran (TCGN). Over 200 students used Tayseer during the first semester, demonstrating its efficiency in streamlining the advisory process. It identified over 50 question types from inputs with a 90% precision in intent and entity predictions. A comprehensive evaluation illuminated Tayseer’s proficiency as well as areas requiring improvement. This study developed an advanced CA to enhance student experiences and satisfaction while establishing best practices for education chatbot interfaces by outlining steps to build an AI-powered chatbot from scratch using techniques adaptable to any language.
Inverses for Fourth-Degree Permutation Polynomials Modulo 32Ψ or 96Ψ, with Ψ as a Product of Different Prime Numbers Greater than Three
Authors: Trifina Lucian , Tărniceriu Daniela , Rotopănescu Ana-Mirela
Published: March 2024

In this paper, we address the inverse of a true fourth-degree permutation polynomial (4-PP), modulo a positive integer of the form 32kLΨ, where kL∈{1,3} and Ψ is a product of different prime numbers greater than three. Some constraints are considered for the 4-PPs to avoid some complicated coefficients’ conditions. With the fourth- and third-degree coefficients of the form k4,fΨ and k3,fΨ, respectively, we prove that the inverse PP is (I) a 4-PP when k4,f∈{1,3} and k3,f∈{1,3,5,7} or when k4,f=2 and (II) a 5-PP when k4,f∈{1,3} and k3,f∈{0,2,4,6}.
The Reliability Inference for Multicomponent Stress–Strength Model under the Burr X Distribution
Authors: Lio Yuhlong , Chen Ding-Geng , Tsai Tzong-Ru , Wang Liang
Published: March 2024

The reliability of the multicomponent stress–strength system was investigated under the two-parameter Burr X distribution model. Based on the structure of the system, the type II censored sample of strength and random sample of stress were obtained for the study. The maximum likelihood estimators were established by utilizing the type II censored Burr X distributed strength and complete random stress data sets collected from the multicomponent system. Two related approximate confidence intervals were achieved by utilizing the delta method under the asymptotic normal distribution theory and parametric bootstrap procedure. Meanwhile, point and confidence interval estimators based on alternative generalized pivotal quantities were derived. Furthermore, a likelihood ratio test to infer the equality of both scalar parameters is provided. Finally, a practical example is provided for illustration.
Thermal Inactivation of the Heat-Resistant Pathogens Salmonella Senftenberg 775W and Escherichia coli AW1.7 in Whey Concentrate
Authors: Fiedler Gregor , Nöbel Stefan , Matzen Sönke , Samtlebe Meike , Franz Charles M. A. P.
Published: March 2024

Pasteurized whey concentrate is used as a base for the production of ingredients for various food products. Whey concentrate (30% dry matter) was used to assess the thermal inactivation of Salmonella (S.) enterica serovar Senftenberg 775W (DSM 10062) and Escherichia (E.) coli AW1.7 (DSM 108612) strains in a pilot-scale pasteurizer mimicking industrial heat processing. These strains, chosen for their exceptional heat resistance, represent the most challenging scenario for pasteurization within the context of S. enterica and E. coli. Heat resistance was tested at temperatures of 56, 60, 64, 68, and 72 °C at an average holding time of 17.5 s. These exceptionally heat-resistant strains showed a relatively low reduction in numbers of between 0 and 4.2 log10 CFU/mL at lower inactivation temperatures of ≤68 °C. A reduction of at least 5 log10 CFU/mL, as required for adequate heat processing, was achieved for both species after heating at 72 °C for 17.5 s. This study shows that whey concentrate should not lead to contamination of food ingredients and can be considered safe after pasteurization at 72 °C for at least 17.5 s with respect to the pathogens tested.
PluMu—A Mu-like Bacteriophage Infecting Actinobacillus pleuropneumoniae
Authors: Bartsch Lee Julia , Fernandez Crespo Roberto , Wang Yunfei , Skinner Michael A. , Rycroft Andrew N. , Cooley William , Everest David J. , Li Yanwen , Bossé Janine T. , Langford Paul R.
Published: March 2024

Actinobacillus pleuropneumoniae is the causative agent of pleuropneumonia, an economically important lung disease in pigs. In draft genomes of two Cypriot clinical A. pleuropneumoniae isolates (MIDG3457 and MIDG3459), we previously identified single genomic regions with homology to Mu-like bacteriophage and presented preliminary evidence of active phage. Here, updated Phastest genomic analysis identified two loci in both MIDG3457 and MIDG3459 that were predicted to encode proteins with high homology to, and whose organisation was characteristic of, Mu-like phages. Phylogenetically, the closest matches were with Mannheimia Vb and Glaesserella SuMu phages. Phastest scored the loci as “complete”, indicating they produced active phage. PCR amplification of the Mu-like phage c and tail genes from DNase-treated polyethylene glycol 8000 (PEG)-precipitated supernatants of MIDG3457 and MIDG3459 (grown in either Brain Heart Infusion-NAD or Grace’s Insect Medium-NAD broth) indicated the presence of intact virions. The phages from MIDG3457 and MIDG3459 were named PluMu 3457-1, 3457-2, and PluMu 3459-1 and PluMu 3459-2, respectively. Transmission electron microscopy (TEM) of the PEG-precipitated supernatants of broth-grown MIDG3459 identified virions with icosahedral heads and tails, consistent with other Mu-like phages. We conclude that MIDG3459 produces an active Mu-like phage.
Landscape Design and Drawing as Tools for Understanding Climate Emergency and Sustainability
Authors: Nikologianni Anastasia
Published: March 2024

Acknowledging the importance of climate challenges to our environment, landscape and cities, this review focuses on the exploration of visual methods (e.g., design, drawing, sketches) in relation to a deeper understanding of climate emergency and sustainability on a spatial scale. It provides an overview of existing research and highlights the role design and drawing can play in landscape-led projects, as well as the impact these might have on behavioural change and decision-making. Looking at how design and drawing are perceived in landscape architecture and what their contribution is to the narrative of a project as well as the decisions made, this paper establishes a connection between pictorial forms and landscape. At the same time, this paper explores whether visualisations are used in relation to the climate and environmental challenges we face, sharing some light on the role they can play with regards to climate awareness and sustainability and how important they might be for our communities, cities and regions. This review highlights the need for further research around this topic and explains that there is a minor part of the literature looking at ways in which landscape design can be integrated into the wider climate emergency agenda and how this might influence behavioural change and the decision-making of various stakeholders.
Three New Species of the Freshwater Shrimp Genus Caridina from Australia
Authors: Klotz Werner , von Rintelen Thomas , von Rintelen Kristina
Published: March 2024

Three new species of the genus Caridina are described from the northernmost part of Australia. Caridina darwin n. sp. resembles Caridina temasek Choy and Ng, 1991 but differs in the armature of the rostrum, the development of epipods on the pereiopods and the absence of an appendix interna on the male first pleopods. Caridina magnovis n. sp. resembles Caridina serratirostris de Man, 1892 but differs in the armature of the ventral margin of the rostrum, a shorter stylocerite, a stouter carpus of the first pereiopod, the number and size of spiniform setae on the third and fifth pereiopods, the shape of the preanal carina and the size of the embryos (referred to as “eggs” in most previous publications). Caridina wilsoni n. sp. resembles Caridina gracilirostris de Man, 1892 but differs in the size of the embryos and in some length to width ratios of the segments of the pereiopods. Detailed morphological descriptions of all three new species are given. A molecular phylogeny (mt DNA 16S) supports the morphospecies hypothesis and illustrates the phylogenetic relationship with morphologically similar species from outside Australia.
Large Vestibular Aqueduct-Associated Symptoms: Endolymphatic Duct Blockage as a Surgical Treatment
Authors: Saliba Issam , Alshehri Sarah , Fournier Isabelle , Altamami Nasser
Published: March 2024

Objective: This study aimed to evaluate the effectiveness of endolymphatic duct blockage (EDB) on dizziness control in patients with a large vestibular aqueduct (LVA) and to evaluate its effect on hearing. Study design: This is a prospective nonrandomized study. Setting: Five adults and one child with dizziness and five children with progressive hearing loss were referred to our tertiary centers. Methods: The dizziness handicap inventory (DHI) and DHI-PC (dizziness handicap inventory—patient caregiver) questionnaires were used before and after surgery. All patients underwent a preoperative temporal bone HRCT scan and pure tone audiometry one day before surgery, then four and twelve months after surgery and at the last follow-up. The mean follow-up time was 5.6 years. Student’s t-test was used to compare DHI/-PC results. Results: The DHI scores were 44, 24, 84, 59 and 56 before surgery, respectively, for Patients 1 to 5. The DHI scores at four months was significantly different, i.e., 4, 6, 0, 7 and 18 (p = 0.001). No differences were found between 4 and 12 months. Patient 6 (child) had Trisomy 21; their DHI-PC score dropped from 38 (preoperative score) to 8 (postoperative score), showing no activity limitations; clinical evaluation showed the complete resolution of symptoms. We found no significant differences between hearing loss before the surgery and at 1 and 12 months post operation for four adult patients. Our fifth adult patient’s hearing changed from severe to profound SNHL. For 5 out of 6 pediatric patients, preoperative PTA and mean ABG were 63 dB and 20 dB, respectively; postoperatively, they improved to 42 dB and 16 dB, respectively. The hearing loss level for the sixth pediatric patient dropped from moderate (PTA = 42 dB) to severe (PTA = 85 dB) due to an opening of the endolymphatic sac and a sudden leak of the endolymph. Conclusions: EDB, using two titanium clips, seems to be helpful for controlling vestibular symptoms and for stabilizing hearing or even to improve hearing in 82% of cases. Nevertheless, there is a risk of hearing worsening.
Automated Detection of Train Drivers’ Head Movements: A Proof-of-Concept Study
Authors: Schackmann David , Bosch Esther
Published: March 2024

With increasing automation in the rail sector, the train driver’s task changes from full control to a supervisory position. This bears the risk of monotony and subsequent changes in visual attention, possibly for the worse. Similar to concepts in car driving, one solution for this could be driver state monitoring with triggered interventions in case of declining task attention. Previous research on train drivers’ visual attention has used eye tracking. In contrast, head tracking is easier to realize within the train driver cabin. This study set out to test whether head tracking is a feasible alternative to eye tracking and can provide similar findings. Based on previous eye-tracking research, we compared differences in head movements in automated vs. manual driving, and for different levels of driving speed and driving experience. We conducted a study with 25 active train drivers in a high-fidelity train simulator. Statistical analyses revealed no significant difference in the vertical head movements between automation levels. There was a significant difference in the horizontal head movements, with train drivers looking more to the right for manual driving. We found no significant influence of driving speed and experience on head movements. Safety implications and the feasibility of head tracking as an alternative to eye tracking are discussed.
3F4 Hypergeometric Functions as a Sum of a Product of 2F3 Functions
Authors: Straton Jack C.
Published: March 2024

This paper shows that certain 3F4 hypergeometric functions can be expanded in sums of pair products of 2F3 functions, which reduce in special cases to 2F3 functions expanded in sums of pair products of 1F2 functions. This expands the class of hypergeometric functions having summation theorems beyond those expressible as pair-products of generalized Whittaker functions, 2F1 functions, and 3F2 functions into the realm of pFq functions where p<q for both the summand and terms in the series. In addition to its intrinsic value, this result has a specific application in calculating the response of the atoms to laser stimulation in the Strong Field Approximation.
Role of Melatonin in Viral, Bacterial and Parasitic Infections
Authors: Maestroni Georges J. M.
Published: March 2024

In all mammals, the circulating pool of MLTs is synthesized in the pineal gland during the night’s darkness hours. Its main function is synchronizing the organism in the photoperiod. In contrast, extra-pineal MLT is synthesized in peripheral organs, does not follow any circadian rhythm or circulate, and plays a detoxifying and cytoprotective role. Circulating MLT may stimulate both innate and acquired immune responses through its circadian action and by activating high-affinity receptors on immunocompetent cells. Extra-pineal MLT may have antioxidant and anti-inflammatory effects that dampen the innate immune response. These two seemingly divergent roles may be considered to be two sides of the same coin. In fact, the integration of both circulating and extra-pineal MLT functions might generate a balanced and effective immune response against microbial pathogens. The studies described in this review investigated the effects of exogenous MLT in various models of infectious diseases using extremely different doses and treatment schedules. None of them evaluated the possibility of integrating the non-circadian anti-inflammatory effect with the circadian immunoenhancing action of MLT. As a consequence, in spite of the fact that most studies agree that MLT has a beneficial effect against infections, it seems difficult to draw any definite conclusion about its possible therapeutic use.
The Cytological Energy Detection of Purulent Inflammation in Synovial Fluid Is Not All Black and White
Authors: Kelbich Petr , Vanaskova Eliska , Hrach Karel , Krejsek Jan , Smisko Frantisek , Hruskova Pavla , Hanuljakova Eva , Novotny Tomas
Published: March 2024

Neutrophils are frequently found in the cytological picture of synovial fluid in several joint pathologies, and a higher proportion of them can even wrongly indicate these cases as purulent inflammation. For reliable differentiation between purulent and non-purulent cases, we use the cytological energy analysis of the synovial fluid. Using this method, we examined 350 knee joint synovial fluid samples. Overall, we found that the percentage of neutrophils ranged between 20.0% and 50.0% in 44 (12.6%) cases and was above 50.0% in 231 (66.0%) cases. In the same group, only 85 (24.3%) highly anaerobic synovial fluid samples were evaluated as purulent inflammation, and another 17 (4.9%) cases were evaluated as very likely purulent inflammation. Further, we quantified the immediate risk of purulent inflammation using the “purulent score” (PS). Of the total of 350 samples, 103 (29.4%) cases were classified as having a very high risk of purulent inflammation (PS = 4), 53 (15.1%) cases were classified as having a significant risk of purulent inflammation (PS = 3), 17 (4.9%) cases were classified as having a moderate risk of purulent inflammation (PS = 2), and 75 (21.4%) cases were classified as having no immediate risk of purulent inflammation (PS = 1). Based on our results and analyses, the cytological energy analysis of synovial fluid is an effective method that can be used to detect and specify joint inflammation and the risk of septic arthritis development.
Correlation between Different Psychological Variables in Women with Fibromyalgia with Symptoms of Neurogenic Inflammation: A Cross-Sectional Study
Authors: Riquelme-Aguado Víctor , Zabarte-del Campo Alazne , Baviano-Klett Guillermo , Fernández-Carnero Josué , Gil-Crujera Antonio , Gómez-Esquer Francisco
Published: March 2024

Fibromyalgia (FM) is a chronic pain syndrome hypothesized to arise from a state of neurogenic inflammation. Mechanisms responsible for pain, as well as psychological variables, are typically altered in this condition. The main objective of this research was to explore somatosensory and psychological alterations in women with FM. The secondary objective was to carry out a secondary analysis to correlate the different variables studied and delve into the influences between them. The relationship between different psychological variables in fibromyalgia is not clear in the previous scientific literature. Forty-four individuals participated, of which twenty-two were controls and twenty-two were women with fibromyalgia. The main outcome measures were the Numeric Pain Rating Scale, Fibromyalgia Impact Questionnaire, pressure pain threshold, conditioned pain modulation, anxiety and depression symptoms, catastrophizing and kinesiophobia cognitions. The main analysis showed that there is a moderate correlation between the psychological variables of depression and fear of movement and the ability to modulate pain. There is also a moderately inverse correlation between pain catastrophizing cognitions and pain intensity/disability. Multiple moderate and strong correlations were found among the various psychological variables studied. FM patients exhibit somatosensory alterations alongside negative psychological symptoms that influence the experience of pain, and they may perpetuate the state of neurogenic inflammation.
Snake Robot with Motion Based on Shape Memory Alloy Spring-Shaped Actuators
Authors: Cortez Ricardo , Sandoval-Chileño Marco Antonio , Lozada-Castillo Norma , Luviano-Juárez Alberto
Published: March 2024

This study presents the design and evaluation of a prototype snake-like robot that possesses an actuation system based on shape memory alloys (SMAs). The device is constructed based on a modular structure of links connected by two degrees of freedom links utilizing Cardan joints, where each degree of freedom is actuated by an agonist–antagonist mechanism using the SMA spring-shaped actuators to generate motion, which can be easily replaced once they reach a degradation point. The methodology for programming the spring shape into the SMA material is described in this work, as well as the instrumentation required for the monitoring and control of the actuators. A simplified design is presented to describe the way in which the motion is performed and the technical difficulties faced in manufacturing. Based on this information, the way in which the design is adapted to generate a feasible robotic system is described, and a mathematical model for the robot is developed to implement an independent joint controller. The feasibility of the implementation of the SMA actuators regarding the motion of the links is verified for the case of a joint, and the change in the shape of the snake robot is verified through the implementation of a set of tracking references based on a central pattern generator. The generated tracking results confirm the feasibility of the proposed mechanism in terms of performing snake gaits, as well as highlighting some of the drawbacks that should be considered in further studies.
Analysis of Structure and Function of Ladybird Leg and Subsequent Design and Fabrication of a Simplified Leg Structure for Robotic Applications
Authors: Mercer Christopher , Hosoda Naoe
Published: March 2024

Many insects are able to walk vertically or upside down on both hard and soft surfaces. In beetles such as the ladybird (Coccinella septempunctata), intermolecular forces between tarsal setae on the footpads of the insects make this movement possible. In prior work, adhesion structures made from polydimethylsiloxane (PDMS) that mimic the action of the tarsal setae have been developed. It is proposed that these adhesion structures could be attached to a simplified version of the leg of a ladybird and used in practical applications. For example, the leg structures could potentially be employed in small surveillance drones to enable attachment to surfaces during flights, in order to preserve battery power. Alternatively, the structures could be used in small robotic devices to enable walking on steeply inclined surfaces. In this program of work, the morphology and movement of the leg of a ladybird were closely studied using a 3D X-ray microscope and a high-speed microscope. The positions of the tendons that facilitated movement were identified. From this knowledge, a simplified leg structure using pin-joints was designed and then fabricated using 3-D printing. The PDMS adhesion structures were then attached to the leg structure. The tendons in the actual insect leg were replicated using thread. Typical detachment forces of about 4 N indicated that the simplified leg structure was, in principle, more than capable of supporting the weight of a small device and then detach successfully. Attachment/detachment movement operations were performed using a linear actuator and controlled remotely. Therefore, proof of concept has been demonstrated for the use of such a simplified ladybird leg structure for the attachment/detachment of small robotic devices to horizontal, inclined, or vertical surfaces.
Correction: Fitong Ketchiwou, G.; Dzansi, L.W. Examining the Impact of Gender Discriminatory Practices on Women’s Development and Progression at Work. Businesses 2023, 3, 347–367
Authors: Fitong Ketchiwou Gaelle , Dzansi Lineo Winifred
Published: March 2024

There was an error in the original publication [...]
A Coordination Polymer Based on Nickel(II)–Cyamelurate: A Robust Catalyst with Highly Dispersed Nickel Sites for Nitrophenol Reduction under Ambient Conditions
Authors: Cruz Taís dos Santos da , Batista Walker Vinícius Ferreira do Carmo , Oliveira Eduarda Ferreira de , Oliveira Wanessa Lima de , Pimentel Dilton Martins , Diab Gabriel Ali Atta , Teixeira Ivo Freitas , Pereira Marcio César , Mesquita João Paulo de
Published: March 2024

Cyamelurate anions obtained from the hydrolysis of polymeric graphitic carbon nitride were used for the preparation of a water-stable and crystalline coordination polymer based on nickel(II)–cyamelurate. The polymer was prepared and applied as a catalyst for the reduction of 4-nitrophenol to 4-aminophenol in the presence of borohydride under ambient conditions. The catalyst was prepared by a simple and environmentally friendly method in an aqueous medium, and it was completely characterized by a variety of techniques, including FTIR, UV–Vis, XRD, TGA, TEM, and STEM. The obtained catalyst was able to catalyze the reaction of 4-nitrophenol to 4-aminophenol with a good kinetic constant. In addition, the catalyst proved to be significantly robust, maintaining a conversion rate greater than 80% after five minutes of reaction for eight consecutive catalytic cycles. In addition, the catalytic activity of the coordination polymer was much higher than that observed for a homogeneous catalyst based on aqueous Ni2+ ions, suggesting the importance of the structure of the coordination sphere formed by the cyamelurate anions. The results presented here can contribute to the application of other coordination polymers anchored with cyamelurate-like ligands and derivatives, as well as to new catalyst designs based on this coordination site formed by oxygen and nitrogen donor atoms.
TNF-Related Apoptosis-Inducing Ligand: Non-Apoptotic Signalling
Authors: Guerrache Abderrahmane , Micheau Olivier
Published: March 2024

TNF-related apoptosis-inducing ligand (TRAIL or Apo2 or TNFSF10) belongs to the TNF superfamily. When bound to its agonistic receptors, TRAIL can induce apoptosis in tumour cells, while sparing healthy cells. Over the last three decades, this tumour selectivity has prompted many studies aiming at evaluating the anti-tumoral potential of TRAIL or its derivatives. Although most of these attempts have failed, so far, novel formulations are still being evaluated. However, emerging evidence indicates that TRAIL can also trigger a non-canonical signal transduction pathway that is likely to be detrimental for its use in oncology. Likewise, an increasing number of studies suggest that in some circumstances TRAIL can induce, via Death receptor 5 (DR5), tumour cell motility, potentially leading to and contributing to tumour metastasis. While the pro-apoptotic signal transduction machinery of TRAIL is well known from a mechanistic point of view, that of the non-canonical pathway is less understood. In this study, we the current state of knowledge of TRAIL non-canonical signalling.
Primary Ciliary Dyskinesia and Retinitis Pigmentosa: Novel RPGR Variant and Possible Modifier Gene
Authors: Baz-Redón Noelia , Sánchez-Bellver Laura , Fernández-Cancio Mónica , Rovira-Amigo Sandra , Burgoyne Thomas , Ranjit Rai , Aquino Virginia , Toro-Barrios Noemí , Carmona Rosario , Polverino Eva , Cols Maria , Moreno-Galdó Antonio , Camats-Tarruella Núria , Marfany Gemma
Published: March 2024

We report a novel RPGR missense variant co-segregated with a familial X-linked retinitis pigmentosa (XLRP) case. The brothers were hemizygous for this variant, but only the proband presented with primary ciliary dyskinesia (PCD). Thus, we aimed to elucidate the role of the RPGR variant and other modifier genes in the phenotypic variability observed in the family and its impact on motile cilia. The pathogenicity of the variant on the RPGR protein was evaluated by in vitro studies transiently transfecting the mutated RPGR gene, and immunofluorescence analysis on nasal brushing samples. Whole-exome sequencing was conducted to identify potential modifier variants. In vitro studies showed that the mutated RPGR protein could not localise to the cilium and impaired cilium formation. Accordingly, RPGR was abnormally distributed in the siblings’ nasal brushing samples. In addition, a missense variant in CEP290 was identified. The concurrent RPGR variant influenced ciliary mislocalisation of the protein. We provide a comprehensive characterisation of motile cilia in this XLRP family, with only the proband presenting PCD symptoms. The variant’s pathogenicity was confirmed, although it alone does not explain the respiratory symptoms. Finally, the CEP290 gene may be a potential modifier for respiratory symptoms in patients with RPGR mutations.
HSP47 Increases the Expression of Type I Collagen in Fibroblasts through IRE1α Activation, XBP1 Splicing, and Nuclear Translocation of β-Catenin
Authors: Ham So Young , Pyo Min Ju , Kang Moonkyung , Kim Yeon-Soo , Lee Dong Hun , Chung Jin Ho , Lee Seung-Taek
Published: March 2024

Heat shock protein 47 (HSP47), also known as SERPINH1, functions as a collagen-specific molecular chaperone protein essential for the formation and stabilization of the collagen triple helix. Here, we delved into the regulatory pathways governed by HSP47, shedding light on collagen homeostasis. Our investigation revealed a significant reduction in HSP47 mRNA levels in the skin tissue of older mice as compared to their younger counterparts. The augmented expression of HSP47 employing lentivirus infection in fibroblasts resulted in an increased secretion of type I collagen. Intriguingly, the elevated expression of HSP47 in fibroblasts correlated with increased protein and mRNA levels of type I collagen. The exposure of fibroblasts to IRE1α RNase inhibitors resulted in the reduced manifestation of HSP47-induced type I collagen secretion and expression. Notably, HSP47-overexpressing fibroblasts exhibited increased XBP1 mRNA splicing. The overexpression of HSP47 or spliced XBP1 facilitated the nuclear translocation of β-catenin and transactivated a reporter harboring TCF binding sites on the promoter. Furthermore, the overexpression of HSP47 or spliced XBP1 or the augmentation of nuclear β-catenin through Wnt3a induced the expression of type I collagen. Our findings substantiate that HSP47 enhances type I collagen expression and secretion in fibroblasts by orchestrating a mechanism that involves an increase in nuclear β-catenin through IRE1α activation and XBP1 splicing. This study therefore presents potential avenues for an anti-skin-aging strategy targeting HSP47-mediated processes.
Fluvastatin Converts Human Macrophages into Foam Cells with Increased Inflammatory Response to Inactivated Mycobacterium tuberculosis H37Ra
Authors: Montero-Vega María Teresa , Matilla Joaquín , Bazán Eulalia , Reimers Diana , De Andrés-Martín Ana , Gonzalo-Gobernado Rafael , Correa Carlos , Urbano Francisco , Gómez-Coronado Diego
Published: March 2024

Cholesterol biosynthesis inhibitors (statins) protect hypercholesterolemic patients against developing active tuberculosis, suggesting that these drugs could help the host to control the pathogen at the initial stages of the disease. This work studies the effect of fluvastatin on the early response of healthy peripheral blood mononuclear cells (PBMCs) to inactivated Mycobacterium tuberculosis (Mtb) H37Ra. We found that in fluvastatin-treated PBMCs, most monocytes/macrophages became foamy cells that overproduced NLRP3 inflammasome components in the absence of immune stimulation, evidencing important cholesterol metabolism/immunity connections. When both fluvastatin-treated and untreated PBMCs were exposed to Mtb H37Ra, a small subset of macrophages captured large amounts of bacilli and died, concentrating the bacteria in necrotic areas. In fluvastatin-untreated cultures, most of the remaining macrophages became epithelioid cells that isolated these areas of cell death in granulomatous structures that barely produced IFNγ. By contrast, in fluvastatin-treated cultures, foamy macrophages surrounded the accumulated bacteria, degraded them, markedly activated caspase-1 and elicited a potent IFNγ/cytotoxic response. In rabbits immunized with the same bacteria, fluvastatin increased the tuberculin test response. We conclude that statins may enhance macrophage efficacy to control Mtb, with the help of adaptive immunity, offering a promising tool in the design of alternative therapies to fight tuberculosis.
Beyond Scanning Electron Microscopy: Comprehensive Pore Analysis in Transparent Ceramics Using Optical Microscopy
Authors: Picelli Francesco , Hostaša Jan , Piancastelli Andreana , Biasini Valentina , Melandri Cesare , Esposito Laura
Published: March 2024

Developing an effective method of quantifying defects in the bulk of transparent ceramics is a challenging task that could facilitate their widespread use as a substitute for single crystals. Conventionally, SEM analysis is used to examine the microstructure but it is limited to the material surface. On the other hand, optical transmittance assesses material quality, but does not provide information on the size and concentration of defects. In this study, we illustrate the use of a digital optical microscope for the non-destructive, precise, and rapid analysis of residual porosity in transparent ceramics. YAG-based ceramics doped with Yb have been selected for this study because they are used as laser gain media, an application that requires virtually defect-free components. Different production processes were used to produce YAG samples, and the digital optical microscope analysis was used to compare them. This analysis was shown to be effective and precise to measure the size and concentration of the residual pores. In addition, the comparison of samples obtained with different production processes showed that the size and distribution of the residual porosity is affected by the drying step of the powders before shaping by pressing, as well as by the sintering aids used to ease the densification. It also showed that the transmittance is influenced by both the total volume and the concentration of the pores.
Structural and Dielectric Properties of Titania Co-Doped with Yttrium and Niobium: Experimental Evidence and DFT Study
Authors: Silva Deborah Y. B. , Muccillo Reginaldo , Muccillo Eliana N. S.
Published: March 2024

This work explores the impact of the sintering temperature and co-dopant contents on the microstructure and dielectric properties of (Y0.5Nb0.5)xTi1−xO2 (0.025 ≤ x ≤ 0.10) ceramics synthesized by the solid state reaction method. The physical mechanism underlying the colossal electric permittivity was systematically investigated with experimental methods and first principles calculations. All specimens exhibited the characteristic tetragonal structure of rutile, besides secondary phases. A niobium- and yttrium-rich secondary phase emerged at the grain boundaries after heating at 1500 °C, changing the main sintering mechanism. The highest value of the electric permittivity (13499 @ 60 °C and 10 kHz) was obtained for (Y0.5Nb0.5)0.05Ti0.95O2 sintered at 1480 °C, and the lowest dissipation factor (0.21@ 60 °C and 10 kHz) for (Y0.5Nb0.5)0.1Ti0.90O2 sintered at 1500 °C. The dielectric properties of Y3+ and Nb5+ co-doped TiO2 are attributed to the internal barrier layer capacitance (IBLC) and electron-pinned dipole defect (EPDD) mechanisms.
Complete Mitochondrial Genomes of Nedyopus patrioticus: New Insights into the Color Polymorphism of Millipedes
Authors: Zhang Gaoji , Xu Tangjun , Chen Yukun , Xu Wei , Wang Yinuo , Li Yuanyuan , Zhu Fuyuan , Liu Hongyi , Ruan Honghua
Published: March 2024

There has been debate about whether individuals with different color phenotypes should have different taxonomic status. In order to determine whether the different color phenotypes of Nedyopus patrioticus require separate taxonomic status or are simply synonyms, here, the complete mitochondrial genomes (mitogenomes) of two different colored N. patrioticus, i.e., red N. patrioticus and white N. patrioticus, are presented. The two mitogenomes were 15,781 bp and 15,798 bp in length, respectively. Each mitogenome contained 13 PCGs, 19 tRNAs, 2 rRNAs, and 1 CR, with a lack of trnI, trnL2, and trnV compared to other Polydesmida species. All genes were located on a single strand in two mitogenomes. Mitochondrial DNA analyses revealed that red N. patrioticus and white N. patrioticus did not show clear evolutionary differences. Furthermore, no significant divergence was discovered by means of base composition analysis. As a result, we suggest that white N. patrioticus might be regarded as a synonym for red N. patrioticus. The current findings confirmed the existence of color polymorphism in N. patrioticus, which provides exciting possibilities for future research. It is necessary to apply a combination of molecular and morphological methods in the taxonomy of millipedes.
Genetic and Environmental Factors in Autoimmune Thyroid Disease: Exploring Associations with Selenium Levels and Novel Loci in a Latvian Cohort
Authors: Upmale-Engela Sabine , Vaivode Ieva , Peculis Raitis , Litvina Helena , Zake Tatjana , Skesters Andrejs , Gogins Deniss , Rovite Vita , Konrade Ilze
Published: March 2024

The interplay of genetic, immune and environmental factors strongly contributes to the development of autoimmune thyroid disease (AITD), which can be classified as Graves’ disease (GD) or Hashimoto thyroiditis (HT). One of the most studied exogenous factors in the pathogenesis of AITD is selenium, which, in the form of selenoproteins, strengthens the antioxidative defence system of thyroid cells against superoxide production. Furthermore, it modulates inflammatory cytokine release and autoantibody production. The aim of this study was to assess the associations of genetic factors with selenium levels in a cohort of adults with HT and GD and healthy controls from Latvia. A total of 148 GD patients, 102 HT patients and 2442 control participants were included in the study. The genotypes were determined using genome-wide genotyping; imputation was carried out using the TOPMed r2 imputation panel; and association analysis was performed with PLINK v1.90b7. We found three loci associated with GD (LSAMP, HNRNPA3P5, and NTN1) and one locus associated with HT (VAT1L); furthermore, one locus was associated with a serum selenium concentration > 80 µg/L (LINC01544/RNF152/PIGN). The detected associations could be attributed to population-specific effects or unknown stratification in our cohort, and further assessment of these results is required to explain the relationships of genetic traits with AITD and other phenotypes.
Autosomal Recessive Rod–Cone Dystrophy with Mild Extra-Ocular Manifestations Due to a Splice-Affecting Variant in BBS9
Authors: Deitch Iris , Itskov Sofia , Panneman Daan , Abu Shtaya Aasem , Saban Tal , Goldberg Yael , Ehrenberg Miriam , Cremers Frans P. M. , Roosing Susanne , Ben-Yosef Tamar
Published: March 2024

Bardet–Biedl syndrome (BBS), one of the most common forms of syndromic inherited retinal diseases (IRDs), is characterized by the combination of retinal degeneration with additional extra-ocular manifestations, including obesity, intellectual disability, kidney disease, polydactyly and other skeletal abnormalities. We observed an Israeli patient with autosomal recessive apparently non-syndromic rod–cone dystrophy (RCD). Extra-ocular findings were limited to epilepsy and dental problems. Genetic analysis with a single molecule molecular inversion probes-based panel that targets the exons and splice sites of 113 genes associated with retinitis pigmentosa and Leber congenital amaurosis revealed a homozygous rare missense variant in the BBS9 gene (c.263C>T;p.(Ser88Leu)). This variant, which affects a highly conserved amino acid, is also located in the last base of Exon 3, and predicted to be splice-altering. An in vitro minigene splice assay demonstrated that this variant leads to the partial aberrant splicing of Exon 3. Therefore, we suggest that this variant is likely hypomorphic. This is in agreement with the relatively mild phenotype observed in the patient. Hence, the findings in our study expand the phenotypic spectrum associated with BBS9 variants and indicate that variants in this gene should be considered not only in BBS patients but also in individuals with non-syndromic IRD or IRD with very mild extra-ocular manifestations.
Effect of the Rehydration Method on the Physical–Mechanical Properties of CO2-Cured Magnesium-Based Fiber Cement Boards
Authors: Azevedo Adriano G. S. , Molano Juan Camilo Adrada , Parente Igor , Freitas Taís O. G. , Camões Aires , Faria Paulina , Savastano Holmer
Published: March 2024

This article analyzes the effect of the rehydration method on the physical–mechanical properties of accelerated carbonation-cured magnesium-based fiber cement boards. The rehydration process of the boards was analyzed in conjunction with the analysis of the pre-curing time before accelerated carbonation (24, 48, and 72 h before carbonation), resulting in eight different curing parameters used in this investigation. The physical–mechanical performance and microstructural characteristics of magnesium oxysulfate boards before and after carbonation were investigated by water absorption, apparent porosity, and bulk density using the four-point bending test, X-ray diffraction, thermogravimetric analysis, and scanning electron microscopy. According to the results, the accelerated carbonation process improved the mechanical properties of the boards. The samples that carbonated after 48 h showed a higher modulus of rupture. The rehydration process of the composites before carbonation led to enhancements in the pre-cured boards for 48 and 72 h, demonstrating that carbonation occurred more effectively after water rehydration. The mechanical improvements were associated with the formation of hydration products, which preferentially formed in the pores and voids of the fiber cement matrix. These carbonation products altered the physical properties of the composites, increasing the density of the boards and reducing the void volume. The decomposition of the formed carbonates was confirmed by thermogravimetric analysis, which indicated that the rehydration process favored the carbonation of the composites.
Temporal and Spatial Analyses of Forest Burnt Area in the Middle Volga Region Based on Satellite Imagery and Climatic Factors
Authors: Kurbanov Eldar , Vorobev Oleg , Lezhnin Sergei , Dergunov Denis , Wang Jinliang , Sha Jinming , Gubaev Aleksandr , Tarasova Ludmila , Wang Yibo
Published: March 2024

Wildfires are important natural drivers of forest stands dynamics, strongly affecting their natural regeneration and providing important ecosystem services. This paper presents a comprehensive analysis of spatiotemporal burnt area (BA) patterns in the Middle Volga region of the Russian Federation from 2000 to 2022, using remote sensing time series data and considering the influence of climatic factors on forest fires. To assess the temporal trends, the Mann–Kendall nonparametric statistical test and Theil–Sen’s slope estimator were applied using the LandTrendr algorithm on the Google Earth Platform (GEE). The accuracy assessment revealed a high overall accuracy (>84%) and F-score value (>82%) for forest burnt area detection, evaluated against 581 reference test sites. The results indicate that fire occurrences in the region were predominantly irregular, with the highest frequency recorded as 7.3 over the 22-year period. The total forest BA was estimated to be around 280 thousand hectares, accounting for 1.7% of the land surface area or 4.0% of the total forested area in the Middle Volga region. Coniferous forest stands were found to be the most fire-prone ecosystems, contributing to 59.0% of the total BA, while deciduous stands accounted for 25.1%. Insignificant fire occurrences were observed in young forests and shrub lands. On a seasonal scale, temperature was found to have a greater impact on BA compared with precipitation and wind speed.
Disruption of Circadian Sleep/Wake Rhythms in Infants May Herald Future Development of Autism Spectrum Disorder
Authors: Miike Teruhisa , Oniki Kentaro , Toyoura Makiko , Tonooka Shiro , Tajima Seiki , Kinoshita Jun , Saruwatari Junji , Konishi Yukuo
Published: March 2024

We investigated whether the abnormal rhythms in infants are related to the future development of autism spectrum disorder (ASD), using a questionnaire from September to October 2016. The parents of 160 children with ASD (male, n = 123; female, n = 37) were recruited from two hospitals in K and H cities, and as a control group, 145 children (male, n = 75; female, n = 70) were recruited from four nursery schools in T city. The associations between ASD and bedtime and waking time on weekdays and weekends in infancy (<1 years of age), at 1–3 years, and at 3–5 years of ages were studied using a multivariable logistic regression analysis. In particular, at <3 years of age, the following factors were associated with an increased prevalence of ASD in the future: (1) short sleep periods (<8 h); (2) taking a long time to fall asleep (>60 min); (3) sleep beginning after 22:00; (4) a wake-up time after 08:00; and (5) frequent (>3 times) and long-term awakening periods (>60 min). The misalignment and/or shift of the circadian rhythm in infants may be one of the precursors and/or risk factors for the future development of ASD.
The Efficacy of a Multimodal Bedroom-Based ‘Smart’ Alarm System on Mitigating the Effects of Sleep Inertia
Authors: Campanella Carolina , Byun Kunjoon , Senerat Araliya , Li Linhao , Zhang Rongpeng , Aristizabal Sara , Porter Paige , Bauer Brent
Published: March 2024

Previous work has demonstrated the modest impact of environmental interventions that manipulate lighting, sound, or temperature on sleep inertia symptoms. The current study sought to expand on previous work and measure the impact of a multimodal intervention that collectively manipulated light, sound, and ambient temperature on sleep inertia. Participants slept in the lab for four nights and were awoken each morning by either a traditional alarm clock or the multimodal intervention. Feelings of sleep inertia were measured each morning through Psychomotor Vigilance Test (PVT) assessments and ratings of sleepiness and mood at five time-points. While there was little overall impact of the intervention, the participant’s chronotype and the length of the lighting exposure on intervention mornings both influenced sleep inertia symptoms. Moderate evening types who received a shorter lighting exposure (≤15 min) demonstrated more lapses relative to the control condition, whereas intermediate types exhibited a better response speed and fewer lapses. Conversely, moderate evening types who experienced a longer light exposure (>15 min) during the intervention exhibited fewer false alarms over time. The results suggest that the length of the environmental intervention may play a role in mitigating feelings of sleep inertia, particularly for groups who might exhibit stronger feelings of sleep inertia, including evening types.