bluuebunny/crossref_metadata_2025 huggingface dataset:
Alterations of gut microbiota in a mouse model with partial small intestinal obstruction
Authors: Wang Yong , Zhang Minzhong , Jiang Lu , Gong Yiming , Liu Keqiang , Zhang Tian
Published: September 2023

Introduction

Changes in the gut microbiota of patients with partial small intestinal obstruction (PSIO) have not been widely clarified. We aimed to explore bacterial diversity in a PSIO mouse model.

Methods

A PSIO mouse model was established using male C57BL/6 mice, and feces samples from the distal ileum and ileum epithelium tissues were collected. MiSeq sequencing of the 16S rRNA gene was conducted to characterize microbiota diversity and composition. RNA sequencing for differences in transcriptomic programming of the ileum tissue was performed between the PSIO and (Control) Ctrl groups.

Results

Bacterial diversity in the PSIO group was significantly lower than that in the controls. Pseudomonadota was predominant in the feces of the PSIO group. Unclassified_Muribaculaceae (p = 0.008) and Akkermansia (p = 0.007) were more abundant in the Ctrl group than those in the PSIO group. Furthermore, Escherichia_Shigella (p = 0.008) was more predominant in the feces of the PSIO group. The Kyoto Encyclopedia of Genes and Genomes pathways related to metabolism were depleted in the PSIO group. Pathways associated with intestinal fibrosis, including extracellular matrix-receptor interaction, focal adhesion, phosphoinositide 3-kinase (PI3K)-Akt signaling pathway and transforming growth factor (TGF)-beta signaling pathway, which were enriched in ileum epithelial tissue in the PSIO group.

Conclusion

PSIO can lead to changes in the predominant intestinal bacterial groups. Depleted functional profiles of the gut microbiota were identified in the PSIO group. Functional pathways associated with intestinal fibrosis were activated by PSIO. The potential regulation by the microbiota needs to be explored in the future.
Developing a genetic approach to target cyanobacterial producers of heterocyte glycolipids in the environment
Authors: Pérez Gallego Ruth , Bale Nicole J. , Sinninghe Damste Jaap S. , Villanueva Laura
Published: September 2023

Heterocytous cyanobacteria are important players in the carbon and nitrogen cycle. They can fix dinitrogen by using heterocytes, specialized cells containing the oxygen-sensitive nitrogenase enzyme surrounded by a thick polysaccharide and glycolipid layer which prevents oxygen diffusion and nitrogenase inactivation. Heterocyte glycolipids can be used to detect the presence of heterocytous cyanobacteria in present-day and past environments, providing insight into the functioning of the studied ecosystems. However, due to their good preservation throughout time, heterocyte glycolipids are not ideal to detect and study living communities, instead methods based on DNA are preferred. Currently cyanobacteria can be detected using untargeted genomic approaches such as metagenomics, or they can be specifically targeted by, for example, the use of primers that preferentially amplify their 16S rRNA gene or their nifH gene in the case of nitrogen fixing cyanobacteria. However, since not all cyanobacterial nitrogen fixers are heterocytous, there is currently no fast gene-based method to specifically detect and distinguish heterocytous cyanobacteria. Here, we developed a PCR-based method to specifically detect heterocytous cyanobacteria by designing primers targeting the gene (hglT) encoding the enzyme responsible for the last step in the biosynthesis of heterocyte glycolipid (i.e., a glycosyltransferase). We designed several primer sets using the publicly available sequences of 23 heterocytous cyanobacteria, after testing them on DNA extracts of 21 heterocyte-forming and 7 non-heterocyte forming freshwater cyanobacteria. The best primer set was chosen and successfully used to confirm the presence of heterocytous cyanobacteria in a marine environmental sample.
Effect of grape pomace supplement on growth performance, gastrointestinal microbiota, and methane production in Tan lambs
Authors: Cheng Xindong , Du Xia , Liang Yanping , Degen Abraham Allan , Wu Xiukun , Ji Kaixi , Gao Qiaoxian , Xin Guosheng , Cong Haitao , Yang Guo
Published: September 2023

Grape pomace (GP), a by-product in wine production, is nutritious and can be used as a feed ingredient for ruminants; however, its role in shaping sheep gastrointestinal tract (GIT) microbiota is unclear. We conducted a controlled trial using a randomized block design with 10 Tan lambs fed a control diet (CD) and 10 Tan lambs fed a pelleted diet containing 8% GP (dry matter basis) for 46 days. Rumen, jejunum, cecum, and colon bacterial and archaeal composition were identified by 16S rRNA gene sequencing. Dry matter intake (DMI) was greater (p < 0.05) in the GP than CD group; however, there was no difference in average daily gain (ADG, p < 0.05) and feed conversion ratio (FCR, p < 0.05) between the two groups. The GP group had a greater abundance of Prevotella 1 and Prevotella 7 in the rumen; of Sharpe, Ruminococcaceae 2, and [Ruminococcus] gauvreauii group in the jejunum; of Ruminococcaceae UCG-014 and Romboutsia in the cecum, and Prevotella UCG-001 in the colon; but lesser Rikenellaceae RC9 gut group in the rumen and cecum, and Ruminococcaceae UCG-005 and Ruminococcaceae UCG-010 in the colon than the CD group. The pathways of carbohydrate metabolism, such as L-rhamnose degradation in the rumen, starch and glycogen degradation in the jejunum, galactose degradation in the cecum, and mixed acid fermentation and mannan degradation in the colon were up-graded; whereas, the pathways of tricarboxylic acid (TCA) cycle VIII, and pyruvate fermentation to acetone in the rumen and colon were down-graded with GP. The archaeal incomplete reductive TCA cycle was enriched in the rumen, jejunum, and colon; whereas, the methanogenesis from H2 and CO2, the cofactors of methanogenesis, including coenzyme M, coenzyme B, and factor 420 biosynthesis were decreased in the colon. The study concluded that a diet including GP at 8% DM did not affect ADG or FCR in Tan lambs. However, there were some potential benefits, such as enhancing propionate production by microbiota and pathways in the GIT, promoting B-vitamin production in the rumen, facilitating starch degradation and amino acid biosynthesis in the jejunum, and reducing methanogenesis in the colon.
The balance between antibiotic resistance and fitness/virulence in Pseudomonas aeruginosa: an update on basic knowledge and fundamental research
Authors: Jordana-Lluch Elena , Barceló Isabel Mª , Escobar-Salom María , Estévez Miguel A. , Zamorano Laura , Gómez-Zorrilla Silvia , Sendra Elena , Oliver Antonio , Juan Carlos
Published: September 2023

The interplay between antibiotic resistance and bacterial fitness/virulence has attracted the interest of researchers for decades because of its therapeutic implications, since it is classically assumed that resistance usually entails certain biological costs. Reviews on this topic revise the published data from a general point of view, including studies based on clinical strains or in vitro-evolved mutants in which the resistance phenotype is seen as a final outcome, i.e., a combination of mechanisms. However, a review analyzing the resistance/fitness balance from the basic research perspective, compiling studies in which the different resistance pathways and respective biological costs are individually approached, was missing. Here we cover this gap, specifically focusing on Pseudomonas aeruginosa, a pathogen that stands out because of its extraordinary capacity for resistance development and for which a considerable number of recent and particular data on the interplay with fitness/virulence have been released. The revised information, split into horizontally-acquired vs. mutation-driven resistance, suggests a great complexity and even controversy in the resistance-fitness/virulence balance in the acute infection context, with results ranging from high costs linked to certain pathways to others that are seemingly cost-free or even cases of resistance mechanisms contributing to increased pathogenic capacities. The elusive mechanistic basis for some enigmatic data, knowledge gaps, and possibilities for therapeutic exploitation are discussed. The information gathered suggests that resistance-fitness/virulence interplay may be a source of potential antipseudomonal targets and thus, this review poses the elementary first step for the future development of these strategies harnessing certain resistance-associated biological burdens.
Multi-dimensional fusion: transformer and GANs-based multimodal audiovisual perception robot for musical performance art
Authors: Lu Shiyi , Wang Panpan
Published: September 2023

Introduction

In the context of evolving societal preferences for deeper emotional connections in art, this paper explores the emergence of multimodal robot music performance art. It investigates the fusion of music and motion in robot performances to enhance expressiveness and emotional impact. The study employs Transformer models to combine audio and video signals, enabling robots to better understand music's rhythm, melody, and emotional content. Generative Adversarial Networks (GANs) are utilized to create lifelike visual performances synchronized with music, bridging auditory and visual perception. Multimodal reinforcement learning is employed to achieve harmonious alignment between sound and motion.

Methods

The study leverages Transformer models to process audio and video signals in robot performances. Generative Adversarial Networks are employed to generate visually appealing performances that align with the musical input. Multimodal reinforcement learning is used to synchronize robot actions with music. Diverse music styles and emotions are considered in the experiments. Performance evaluation metrics include accuracy, recall rate, and F1 score.

Results

The proposed approach yields promising results across various music styles and emotional contexts. Performance smoothness scores exceed 94 points, demonstrating the fluidity of robot actions. An accuracy rate of 95% highlights the precision of the system in aligning robot actions with music. Notably, there is a substantial 33% enhancement in performance recall rate compared to baseline modules. The collective improvement in F1 score emphasizes the advantages of the proposed approach in the realm of robot music performance art.

Discussion

The study's findings demonstrate the potential of multimodal robot music performance art in achieving heightened emotional impact. By combining audio and visual cues, robots can better interpret and respond to music, resulting in smoother and more precise performances. The substantial improvement in recall rate suggests that the proposed approach enhances the robots' ability to accurately mirror the emotional nuances of the music. These results signify the potential of this approach to transform the landscape of artistic expression through robotics, opening new avenues for emotionally resonant performances.
Left atrial area index provides the best prediction of atrial fibrillation in ischemic stroke patients: results from the LAETITIA observational study
Authors: Alonso Angelika , Kraus Josephine , Ebert Anne , Nikolayenko Valeriya , Kruska Mathieu , Sandikci Vesile , Lesch Hendrik , Duerschmied Daniel , Platten Michael , Baumann Stefan , Szabo Kristina , Akin Ibrahim , Fastner Christian
Published: September 2023

Background and aims

Left atrial (LA) enlargement has been repeatedly shown to be associated with the diagnosis of atrial fibrillation (AF). In clinical practice, several parameters are available to determine LA enlargement: LA diameter index (LADI), LA area index (LAAI), or LA volume index (LAVI). We investigated the predictive power of these individual LA parameters for AF in patients with acute ischemic stroke or transient ischemic attack (TIA).

Methods

LAETITIA is a retrospective observational study that reflects the clinical reality of acute stroke care in Germany. Consecutive patient cases with acute ischemic cerebrovascular event (CVE) in 2019 and 2020 were identified from the Mannheim stroke database. Predictive power of each LA parameter was determined by the area under the curve (AUC) of receiver operating characteristic curves. A cutoff value was determined. A multiple logistic regression analysis was performed to confirm the strongest LA parameter as an independent predictor of AF in patients with acute ischemic CVE.

Results

A total of 1,910 patient cases were included. In all, 82.0% of patients had suffered a stroke and 18.0% had a TIA. Patients presented with a distinct cardiovascular risk profile (reflected by a CHA2DS2-VASc score ≥2 prior to hospital admission in 85.3% of patients) and were moderately affected on admission [median NIHSS score 3 (1; 8)]. In total, 19.5% of patients had pre-existing AF, and 8.0% were newly diagnosed with AF. LAAI had the greatest AUC of 0.748, LADI of 0.706, and LAVI of 0.719 (each p < 0.001 vs. diagonal line; AUC-LAAI vs. AUC-LADI p = 0.030, AUC-LAAI vs. AUC-LAVI p = 0.004). LAAI, increasing NIHSS score on admission, and systolic heart failure were identified as independent predictors of AF in patients with acute ischemic CVE. To achieve a clinically relevant specificity of 70%, a cutoff value of ≥10.3 cm2/m2 was determined for LAAI (sensitivity of 69.8%).

Conclusion

LAAI revealed the best prediction of AF in patients with acute ischemic CVE and was confirmed as an independent risk factor. An LAAI cutoff value of 10.3 cm2/m2 could serve as an inclusion criterion for intensified AF screening in patients with embolic stroke of undetermined source in subsequent studies.
Secondary headaches in pregnancy and the puerperium
Authors: Khoromi Suzan
Published: September 2023

Headache during pregnancy can be due to primary causes such as migraine but can also be a presenting symptom of secondary causes including life threatening conditions. This is a minireview of secondary causes of headache during pregnancy and the puerperium. Unique alterations in physiological and vascular functions as well as in the coagulation pathway which occur during pregnancy increase the risk of most of these secondary conditions which include preeclampsia, eclampsia, hemorrhagic stroke, cerebral venous, sinus thrombosis, reversible cerebral vascular syndrome, and posterior reversible encephalopathy. Marked increase in progesterone level in pregnancy is also associated with the growth of tumors such as meningiomas, as 70% of these tumors are positive for progesterone receptors and increase in size can lead to headache along with other neurological symptoms. Hemodynamic changes can lead to the growth of meningiomas as well. Although hormone producing pituitary tumors are usually not conducing to pregnancy, women with known pituitary tumors who do get pregnant may become symptomatic during pregnancy and develop secondary headache. Another rare cause of secondary headache during pregnancy is pituitary apoplexy. Although its occurrence is uncommon, it needs to be properly recognized and treated to avoid endocrine and visual complications. Other rare entities with increased incidence during the puerperium such postdural puncture headache will be also discussed. In summary, new onset headache during pregnancy deserves special attention because in the absence of proper recognition and treatment, secondary headache disorders can endanger the life of the mother and the fetus.
CRISPR deletion of a SINE-VNTR-Alu (SVA_67) retrotransposon demonstrates its ability to differentially modulate gene expression at the MAPT locus
Authors: Fröhlich Alexander , Hughes Lauren S. , Middlehurst Ben , Pfaff Abigail L. , Bubb Vivien J. , Koks Sulev , Quinn John P.
Published: September 2023

Background

SINE-VNTR-Alu (SVA) retrotransposons are hominid-specific elements which have been shown to play important roles in processes such as chromatin structure remodelling and regulation of gene expression demonstrating that these repetitive elements exert regulatory functions. We have previously shown that the presence or absence of a specific SVA element, termed SVA_67, was associated with differential expression of several genes at the MAPT locus, a locus associated with Parkinson’s Disease (PD) and frontotemporal dementia. However, we were not able to demonstrate that causation of differential gene expression was directed by the SVA due to lack of functional validation.

Methods

We performed CRISPR to delete SVA_67 in the HEK293 cell line. Quantification of target gene expression was performed using qPCR to assess the effects on expression in response to the deletion of SVA_67. Differences between CRISPR edit and control cell lines were analysed using two-tailed t-test with a minimum 95% confidence interval to determine statistical significance.

Results

In this study, we provide data highlighting the SVA-specific effect on differential gene expression. We demonstrate that the hemizygous deletion of the endogenous SVA_67 in CRISPR edited cell lines was associated with differential expression of several genes at the MAPT locus associated with neurodegenerative diseases including KANSL1, MAPT and LRRC37A.

Discussion

This data is consistent with our previous bioinformatic work of differential gene expression analysis using transcriptomic data from the Parkinson’s Progression Markers Initiative (PPMI) cohort. As SVAs have regulatory influences on gene expression, and insertion polymorphisms contribute to interpersonal differences in expression patterns, these results highlight the potential contribution of these elements to complex diseases with potentially many genetic components, such as PD.
Web-based processing of physiological noise in fMRI: addition of the PhysIO toolbox to CBRAIN
Authors: Valevicius Darius , Beck Natacha , Kasper Lars , Boroday Sergiy , Bayer Johanna , Rioux Pierre , Caron Bryan , Adalat Reza , Evans Alan C. , Khalili-Mahani Najmeh
Published: September 2023

Neuroimaging research requires sophisticated tools for analyzing complex data, but efficiently leveraging these tools can be a major challenge, especially on large datasets. CBRAIN is a web-based platform designed to simplify the use and accessibility of neuroimaging research tools for large-scale, collaborative studies. In this paper, we describe how CBRAIN’s unique features and infrastructure were leveraged to integrate TAPAS PhysIO, an open-source MATLAB toolbox for physiological noise modeling in fMRI data. This case study highlights three key elements of CBRAIN’s infrastructure that enable streamlined, multimodal tool integration: a user-friendly GUI, a Brain Imaging Data Structure (BIDS) data-entry schema, and convenient in-browser visualization of results. By incorporating PhysIO into CBRAIN, we achieved significant improvements in the speed, ease of use, and scalability of physiological preprocessing. Researchers now have access to a uniform and intuitive interface for analyzing data, which facilitates remote and collaborative evaluation of results. With these improvements, CBRAIN aims to become an essential open-science tool for integrative neuroimaging research, supporting FAIR principles and enabling efficient workflows for complex analysis pipelines.
Isolation of the murine Glut1 deficient thalamocortical circuit: wavelet characterization and reverse glucose dependence of low and gamma frequency oscillations
Authors: Solis Elysandra M. , Good Levi B. , Vázquez Rafael Granja , Patnaik Sourav , Hernandez-Reynoso Ana G. , Ma Qian , Angulo Gustavo , Dobariya Aksharkumar , Cogan Stuart F. , Pancrazio Joseph J. , Pascual Juan M. , Jakkamsetti Vikram
Published: September 2023

Glucose represents the principal brain energy source. Thus, not unexpectedly, genetic glucose transporter 1 (Glut1) deficiency (G1D) manifests with encephalopathy. G1D seizures, which constitute a prominent disease manifestation, often prove refractory to medications but may respond to therapeutic diets. These seizures are associated with aberrant thalamocortical oscillations as inferred from human electroencephalography and functional imaging. Mouse electrophysiological recordings indicate that inhibitory neuron failure in thalamus and cortex underlies these abnormalities. This provides the motivation to develop a neural circuit testbed to characterize the mechanisms of thalamocortical synchronization and the effects of known or novel interventions. To this end, we used mouse thalamocortical slices on multielectrode arrays and characterized spontaneous low frequency oscillations and less frequent 30–50 Hz or gamma oscillations under near-physiological bath glucose concentration. Using the cortical recordings from layer IV among other regions recorded, we quantified oscillation epochs via an automated wavelet-based algorithm. This method proved analytically superior to power spectral density, short-time Fourier transform or amplitude-threshold detection. As expected from human observations, increased bath glucose reduced the lower frequency oscillations while augmenting the gamma oscillations, likely reflecting strengthened inhibitory neuron activity, and thus decreasing the low:high frequency ratio (LHR). This approach provides an ex vivo method for the evaluation of mechanisms, fuels, and pharmacological agents in a crucial G1D epileptogenic circuit.
Using the Research Domain Criteria as a framework to integrate psychophysiological findings into stress management and psychotherapy interventions
Authors: Steffen Patrick R.
Published: September 2023

Research on the psychophysiology of stress is expanding rapidly, but the field lacks a clear integrative framework to help translate research findings into empirically supported stress interventions. The Research Domain Criteria (RDoC) is an excellent candidate to explore as a framework to integrate stress research. The RDoC framework is a dimensional, multi-modal approach to psychopathology proposed as an alternative to categorical approaches used by the International Classification of Diseases (ICD) and the Diagnostic and Statistical Manual (DSM). The goal of this paper is to explore the RDoC as a framework to integrate psychophysiology research into therapeutic interventions for stress. The RDoC consists of six domains: negative valence systems, positive valence systems, cognitive systems, social processes systems, arousal/regulatory systems, and sensorimotor systems, and provides an excellent structure for integrating information from multiple levels of functioning including physiology, behavior, and self-report, as well as genes, molecules, cells, and brain circuits. Integrating psychophysiological research on stress using the RDoC framework can direct and amplify stress management and psychotherapeutic interventions. First, the RDoC provides a clear foundation for conceptualizing the stress response in terms of important concepts such as allostasis and adaptation. In this perspective, the terms “allostatic response” or “adaptation response” are more descriptive terms than “stress response” in understanding bodily responses to life threats and challenges. Second, psychophysiological approaches can be used in the context of modalities such as biofeedback and mindfulness to both collect psychophysiological data and then integrate that data into a broader therapeutic framework. Heart rate variability (HRV) biofeedback is being used more frequently as part of a therapeutic intervention package with stress management and psychotherapy, and HRV data is also used to provide outcome evidence on the efficacy of treatment. Mindfulness practices are commonly used in combination with stress management and psychotherapy, and psychophysiological data (HRV, EEG, blood pressure, etc.) is often collected to explore and understand mind/body relationships. In conclusion, the lack of a clear framework to assess and understand mind/body functioning limits current stress research and interventions. The RDoC provides a strong framework to assess and integrate physiological and psychological data and improve stress interventions.
Association between dietary consumption patterns and the development of adolescent overnutrition in eastern Ethiopia: new perspectives
Authors: Gedamu Fikerte , Dagne Imam , Oumer Abdu
Published: September 2023

Background

Overnutrition among adolescents is becoming a major public health concern, with all the adverse consequences associated with unhealthy eating behaviors. Hence, clear evidence linking dietary consumption with the risk of overnutrition is crucial for targeted dietary recommendations using a robust statistical approach. This study assessed the link between dietary consumption patterns and the risks of overnutrition among adolescents in Ethiopia.

Method

A community-based survey was conducted on a random sample of 510 adolescents selected using a stratified random sampling via proportional allocation. Dietary consumption was captured using a validated and contextualized 80-item food frequency questionnaire over the past month through a face-to-face interview. Weight and height were measured under a standard procedure. Body Mass Index for Age Z-score (BAZ) was calculated, and BAZ above +1 was considered overnutrition. The frequency measures were standardized into daily equivalents, and dietary patterns were derived using exploratory factor analysis after checking for assumptions. A bivariable and multivariable binary logistic regression model was fitted with an odds ratio and 95% confidence intervals.

Results

A total of 510 participants were enrolled. Four major dietary patterns (“cereals, energy, and discretionary calory,” “fat, oil, and milk groups,” “proteins and vegetables,” and “fruits”), explaining 66.6% of the total variation, were identified. The overall prevalence of overnutrition was 29.0% (27–31%), where 22.5 and 6.5% were overweight and obese, respectively. Physical inactivity (AOR = 6.27; 95% CI: 2.75–14.3), maternal literacy (AOR = 111.3; 95% CI: 50.0–247.8), habit of snacking (AOR = 1.80; 95% CI: 0.69–4.67), skipping meals (AOR = 2.05; 955 CI: 0.84–5.04), cereals and discretionary food dietary pattern (AOR = 2.28; 95 CI: 0.94–5.55), and protein–rich and vegetable dietary pattern (AOR = 2.30; 95% CI: 0.97–5.46) were important factors associated with odds of overnutrition.

Conclusion

Overnutrition is a public health concern affecting one-third of adolescents, and it is closely linked with dietary consumption patterns, eating behaviors, wealth status, literacy, and level of physical activity. Therefore, public health interventions targeting unhealthy eating and lifestyles are urgently needed to curb the increasing burden of overnutrition among adolescents and its future complications.
PITX1 plays essential functions in cancer
Authors: Zhao Jingpu , Xu Yongfeng
Published: September 2023

PITX1, also known as the pituitary homeobox 1 gene, has emerged as a key regulator in animal growth and development, attracting significant research attention. Recent investigations have revealed the implication of dysregulated PITX1 expression in tumorigenesis, highlighting its involvement in cancer development. Notably, PITX1 interacts with p53 and exerts control over crucial cellular processes including cell cycle progression, apoptosis, and chemotherapy resistance. Its influence extends to various tumors, such as esophageal, colorectal, gastric, and liver cancer, contributing to tumor progression and metastasis. Despite its significance, a comprehensive review examining PITX1’s role in oncology remains lacking. This review aims to address this gap by providing a comprehensive overview of PITX1 in different cancer types, with a particular focus on its clinicopathological significance.
Towards high-resolution quantitative assessment of vascular dysfunction
Authors: Guidi Maria , Giulietti Giovanni , Biondetti Emma , Wise Richard , Giove Federico
Published: September 2023

Neurovascular alterations are increasingly recognized as a key feature of many brain diseases. They can manifest as a reduction in resting cerebral blood flow or cerebrovascular reactivity (CVR) in the whole brain or in specific regions, depending on the underlying condition. Neurovascular impairment is observed in hypertension, Alzheimer’s disease, stroke, multiple sclerosis and cerebral small vessel disease. Magnetic resonance imaging (MRI)-derived CVR mapping is a reliable marker of vascular dysfunction and has been performed mainly at standard functional MRI (fMRI) resolutions of 2–3 mm using the blood oxygen level dependent (BOLD) contrast. However, vascular alterations may occur at a finer scale (i.e., in the capillary bed) which would be better characterized with smaller voxel sizes. Capillaries in gray matter deliver oxygen and glucose to neural tissue and are arranged in a mesh structure, with variable density across the cortical depth. Given that the human cortex is, on average, 2.5 mm thick, submillimetric voxel sizes are effective in increasing the spatial specificity of measurements of hemodynamic and metabolic changes. Novel MRI sequences offer the possibility to map physiological parameters at high resolution with relatively simple experimental setups. In particular, pairing the BOLD acquisition with a contrast sensitive to blood volume changes, while administering a mild hypercapnic challenge, allows for simultaneous mapping of CVR, cerebral metabolic rate of oxygen consumption and other relevant parameters at a high resolution and can be performed at the clinical field strength of 3 T. We propose that this approach will help provide crucial insights into vascular impairment.
Modeling of the high-resolution optical-coherence diagnostics of bi-refringent biological tissues
Authors: Angelsky O. V. , Bekshaev A. Ya. , Zenkova C. Yu. , Ivanskyi D. I. , Zheng J. , Chumak M. M.
Published: September 2023

We present a computer model of the polarization-sensitive interference diagnostics of the bi-refringent biological media, with a particular example of the lamella of eye cornea. The diagnostic procedure employs the modified Mach–Zehnder interferometer with controllable phase retardation of the reference wave, separate observation of the orthogonal linearly-polarized interference signals, and evaluation of the phases and amplitudes of their variable (AC) components. The data obtained permit to determine the mean refractive index as well as the difference between the extraordinary and ordinary refractive indices, which, in turn, indicates the optical axis and the collagen fibers’ orientation in the lamella. The modelled procedure enables the sample structure diagnostics with the longitudinal and lateral resolution ∼100 nm and ∼1.8 μm, correspondingly. In particular, it permits a reliable detection and quantitative characterization of a thin (<100 nm) near-surface layer where the mean refractive index differs by less than 1% from that in the main volume (due to the different orientation of the collagen fibers). The diagnostic approach, developed in the paper, can be useful in various problems of structure characterization of optically-anisotropic biological tissues.
Multiple description coding ghost imaging
Authors: Zhang Yi , Zhang Yunhe , Chang Chen , Sun Shuai , Liu Weitao
Published: September 2023

Ghost imaging (GI) reveals its exceptional superiority over conventional cameras in a range of challenging scenarios such as weak illumination or special waveband. For high-performance GI, it is vital to obtain a sequence of high-fidelity bucket signals. However, measurements may suffer from distortion or loss in harsh environments. Here we present multiple description coding ghost imaging, which rests on illumination consisting of different coding patterns to address this challenge. Experimental results indicate that the proposed method is capable of producing satisfactory image even when the sequence of bucket signals is incomplete or highly distorted. This method provides an encouraging boost for GI in practical applications.
Environmental and anthropogenic drivers of watercress (Nasturtium officinale) communities in char-lands and water channels across the Swat River Basin: implication for conservation planning
Authors: Khan Nasrullah , Ullah Rafi , Okla Mohammad K. , Abdel-Maksoud Mostafa A. , Saleh Ibrahim A. , Abu-Harirah Hashem A. , AlRamadneh Tareq Nayef , AbdElgawad Hamada
Published: September 2023

Recent anthropogenic sources and excess usage have immensely threatened the communities and habitat ecology of this region’s medicinally and economically significant crops. Therefore, our study aims to evaluate the community structure and related environmental characteristics sustaining Nasturtium officinale communities along the river basin (RB) in Northwest Pakistan, using the clustering procedure (Ward’s method) and Redundancy analysis (RDA). From 340 phytosociological plots (34 × 10 = 340), we identified four ecologically distinct assemblages of N. officinale governed by different environmental and anthropogenic factors for the first time. The floristic structure shows the dominance of herbaceous (100%), native (77%), and annual (58.09%) species indicating relatively stable communities; however, the existence of the invasive plants (14%) is perturbing and may cause instability in the future, resulting in the replacement of herbaceous plant species. Likewise, we noticed apparent variations in the environmental factors, i.e., clay percentage (p = 3.1 × 10−5), silt and sand percentage (p< 0.05), organic matter (p< 0.001), phosphorus and potassium (p< 0.05), and heavy metals, i.e., Pb, Zn, and Cd (p< 0.05), indicating their dynamic role in maintaining the structure and composition of these ecologically distinct communities. RDA has also demonstrated the fundamental role of these factors in species–environment correlations and explained the geospatial variability and plants’ ecological amplitudes in the Swat River wetland ecosystem. We concluded from this study that N. officinale communities are relatively stable due to their rapid colonization; however, most recent high anthropogenic interventions especially overharvesting and sand mining activities, apart from natural enemies, water deficit, mega-droughts, and recent flood intensification due to climate change scenario, are robust future threats to these communities. Our research highlights the dire need for the sustainable uses and conservation of these critical communities for aesthetics, as food for aquatic macrobiota and humans, enhancing water quality, breeding habitat, fodder crop, and its most promising medicinal properties in the region.
Authenticating the geographic origins of Atractylodes lancea rhizome chemotypes in China through metabolite marker identification
Authors: Zhang Chengcai , Wang Hongyang , Lyu Chaogeng , Wang Yiheng , Sun Jiahui , Zhang Yan , Xiang Zengxu , Guo Xiuzhi , Wang Yuefeng , Qin Ming , Wang Sheng , Guo Lanping
Published: September 2023

Introduction

Atractylodes lancea is widely distributed in East Asia, ranging from Amur to south-central China. The rhizome of A. lancea is commonly used in traditional Chinese medicine, however, the quality of products varies across different regions with different geochemical characteristics.

Method

This study aimed to identify the chemotypes of A. lancea from different areas and screen for chemical markers by quantifying volatile organic compounds (VOCs) using a targeted metabolomics approach based on GC–MS/MS.

Results

The A. lancea distributed in Hubei, Anhui, Shaanxi, and a region west of Henan province was classified as the Hubei Chemotype (HBA). HBA is characterized by high content of β-eudesmol and hinesol with lower levels of atractylodin and atractylon. In contrast, the Maoshan Chemotype (MA) from Jiangsu, Shandong, Shanxi, Hebei, Inner Mongolia, and other northern regions, exhibited high levels of atractylodin and atractylon. A total of 15 categories of VOCs metabolites were detected and identified, revealing significant differences in the profiles of terpenoid, heterocyclic compound, ester, and ketone among different areas. Multivariate statistics indicated that 6 compounds and 455 metabolites could serve as candidate markers for differentiating A. lancea obtained from the southern, northern, and Maoshan areas.

Discussion

This comprehensive analysis provides a chemical fingerprint of selected A. lancea. Our results highlight the potential of metabolite profiling combined with chemometrics for authenticating the geographical origin of A. lancea.
Psychometric properties of the Chinese version of the Trait Gratitude to Nature Scale
Authors: Li Xiaoyu , Liang Hongyu , Jin Tonglin , Zhang Jing , A Yunna , Hu Mulan , Wang Yifan
Published: September 2023

Objective

The purpose of this study was to evaluate the reliability and validity of the Chinese version of the Trait Gratitude to Nature Scale (TGNS) for Chinese college students.

Methods

The original English version of the TGNS was translated into Chinese. Subsequently, two samples consisting of 1,131 Chinese university students from Inner Mongolia Autonomous Region was recruited through online surveys to evaluate the psychometric properties of the Chinese version of the TGNS, including the discrimination, construct validity, criterion validity, reliability and gender invariance.

Results

The Chinese version of the TGNS showed good psychometric properties. The item-total correlation coefficients of the scale ranged from 0.813 to 0.909. Exploratory factor analysis using data from Sample 1 (n = 617) demonstrated that the Chinese version of the TGNS has one factor. The confirmatory factor analysis using data from Sample 2 (n = 514) showed that the Chinese version of the TGNS has appropriate construct validity (χ2/df = 4.157, RMSEA = 0.078, TLI = 0.943 and CFI = 0.967). The significant correlation between the Chinese version of the TGNS and all the other criterion scale scores (p < 0.001) indicated that the Chinese version of the TGNS displays good criterion validity. The test–retest reliability was 0.914, using the sub-sample of Sample 2 (n = 127). The results of gender invariance test indicated that the Chinese version of the TGNS has entire equivalence between the two genders.

Conclusion

The Chinese version of the TGNS has satisfactory psychometric properties in the Chinese cultural context and can be used as s a reliable and valid instrument to assess trait gratitude to nature.
Mediating role of splitting in relation to attachment styles and shopping addiction
Authors: Allahvirdie Rezaieh Sarah , Ghorbani Nima , Farahani Hojjatollah
Published: September 2023

Introduction

Shopping can provide a sense of satisfaction and pleasure; however, if a person’s excessive involvement in this behavior starts to negatively impact other aspects of their life, similar to other addictive behaviors like excessive internet use, gaming, and gambling, it may be classified as pathological. Given the lack of agreement regarding the classification of excessive shopping tendencies as a separate mental health condition or addictive behavior, taking a socio-emotional approach to explore the psychological factors that precede this behavior, may offer a better comprehension of it.

Methods

The purpose of this study was to examine the relationship between attachment styles and excessive shopping behavior, as well as to investigate the potential mediating effect of defense mechanisms like splitting on this relationship. Using convenience sampling, a group of 457 stock market employees (116 female, 341 male) between the ages of 24 and 60 were recruited. The researchers utilized a set of validated psychological questionnaires to assess the employees attachment styles, shopping addiction, and splitting tendencies.

Results

The results obtained from both the mediation model and path analysis suggest that attachment styles do not have a direct relationship with shopping addiction. Nonetheless, the study supports the impact of insecure anxious and avoidan attachment styles on splitting. Furthermore, the findings confirm that splitting has a mediating effect on the relationship between attachment styles and splitting.

Discussion

The present study enhanced our comprehension of the subconscious mechanisms underlying shopping tendencies. Specifically, the findings suggest that excessive tendencies towards shopping can be considered a maladaptive response resulting from insecure attachment styles and the unconscious utilization of the splitting defense mechanism.
Body-related concerns and participation in physical education among adolescent students: the mediating role of motivation
Authors: Haug Ellen , Castillo Isabel , Samdal Oddrun , Smith Otto Robert Frans
Published: September 2023

Background

There is a need to understand better factors influencing participation in physical education (PE) and the mechanisms involved. The adolescent years are characterised by increasing levels of body-related concerns. In PE, the body is judged for its physical abilities and subject to social comparisons and body judgements. Grounded in the Self-Determination Theory, this study aimed to explore whether body-related factors were associated with adolescents’ involvement in PE and whether types of motivation mediated this relationship.

Methods

The study involved 2,140 (54.5% girls) secondary students (15–16-year-olds) from Norway participating in the nationally representative “Health Behaviour in School-aged Children (HBSC) study: a WHO collaborative cross-national study.” Body-related factors included Body Mass Index (BMI), health complaints, body perception and dietary behaviours. Gender, age, and socioeconomic status (family affluence) were control variables. Motivation for PE was assessed with the Perceived Locus of Causality (PLOCQ) scale measuring three distinct factors: autonomous motivation, controlled motivation and amotivation. PE involvement was self-reported as weekly participation in PE classes and time spent in moderate-to-vigorous physical activity (MVPA) during PE.

Results

Gender (girl), family affluence, health complaints, not being on a diet but wanting to lose weight, and body perception (too fat) were negatively associated with weekly PE participation when adjusting for other variables. This association was largely explained by students’ autonomous motivation in the case of health complaints and partly in the case of dietary behaviour and body perception. Similar results were observed for MVPA during PE lessons. Additionally, gender was associated with MVPA through amotivation.

Conclusion

The study adds new knowledge to the understanding of the relationship between body-related factors and PE, supporting that autonomous motivation is a central mechanism and an avenue for further research. The results should be considered in planning high-quality PE classes and suggest that an autonomous supportive learning climate sensitive to body-related concerns should be a priority to increase adolescent involvement in PE.
A conceptual framework for how structural changes in emerging acute substance use service models can reduce stigma of medications for opioid use disorder
Authors: Becker Timothy D. , Eschliman Evan L. , Thakrar Ashish P. , Yang Lawrence H.
Published: September 2023

Stigma toward people taking medication for opioid use disorder (MOUD) is prevalent, harmful to the health and well-being of this population, and impedes MOUD treatment resource provision, help-seeking, and engagement in care. In recent years, clinicians have implemented new models of MOUD-based treatment in parts of the United States that integrate buprenorphine initiation into emergency departments and other acute general medical settings, with post-discharge linkage to office-based treatment. These service models increase access to MOUD and they have potential to mitigate stigma toward opioid use and MOUD. However, the empirical literature connecting these emerging service delivery models to stigma outcomes remains underdeveloped. This paper aims to bridge the stigma and health service literatures via a conceptual model delineating how elements of emerging MOUD service models can reduce stigma and increase behavior in pursuit of life goals. Specifically, we outline how new approaches to three key processes can counter structural, public, and self-stigma for this population: (1) community outreach with peer-to-peer influence, (2) clinical evaluation and induction of MOUD in acute care settings, and (3) transition to outpatient maintenance care and early recovery. Emerging service models that target these three processes can, in turn, foster patient empowerment and pursuit of life goals. There is great potential to increase the well-being of people who use opioids by reducing stigma against MOUD via these structural changes.
A baseline sarcopenia index based on creatinine/cystatin C for the prediction of stroke recurrence and mortality in older survivors of first ischemic strokes
Authors: Hou Lisha , Chen Xiaoyan , Zhu Cairong
Published: September 2023

Objective

Older adults individuals have a higher risk of stroke recurrence, leading to high mortality and disability rates, which, in turn, hinders the achievement of healthy aging. This study aimed to assess the utility of a baseline sarcopenia index (SI) based on serum creatinine (Cr)/cystatin C (CysC) as a prognostic marker for the risk of stroke recurrence and mortality in first-ever ischemic stroke older survivors (ISOS).

Materials and methods

Data were obtained from an ischemic stroke cohort study. The baseline information was collected from medical records and face-to-face interviews with patients admitted between January 2010 and June 2016. Follow-up information was obtained from telephone interviews every 3 months to determine stroke recurrence and survival status. The SI was calculated from the Cr and CysC values in the medical records as Cr/CysC × 100. Using the first quantile of the SI as the cut-off value, the study participants were divided into the low muscle-mass group (low SI) and the high muscle-mass group (high SI). Cox regression analysis was used to assess the association between SI and recurrence and mortality.

Results

A total of 415 first-ever ISOS were enrolled, including 242 (58.31%) male and 173 (41.69%) female participants. In the high-SI group, the relapse and mortality rates were lower than those in the low-SI group (relapse: 20.58% vs. 30.77%; mortality:13.5% vs. 29.81%). After adjusting for confounding factors, the high-SI group was found to have a lower risk of relapse and mortality than the low-SI group (relapse: HR = 0.571; mortality: HR = 0.294).

Conclusion

The SI was predictive of the long-term prognosis of IS recurrence and mortality in first-ever ISOS. After discharge, in addition to conventional medication, it is recommended that patients with low SI values actively receive treatment for sarcopenia to reduce the risk of stroke recurrence and mortality and promote healthy aging.