bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Rivet Arrangement on the Strengths of Lap Joints and Lap Joint Design Methods
Authors: Ren Kerong , Han Haobing , Xu Wentao , Qing Hua
Published: May 2023

To address the impact of rivet arrangement on the strengths of riveted lap joints, the failure modes and failure mechanisms of riveted lap joints were first studied using finite element analysis software. Next, the effects of the number of rivets, rivet rows, rivet arrangement, and row spacing on the lap joint strength were studied using the peak load as the evaluation index. Then, we proposed the concept of line load density to solve the problem that a varying rivet spacing and rivet edge distance will change the width of the sheet and thus the maximum load capacity, which is used as an index to study the effect of rivet spacing and rivet edge distance on the lap strength. Finally, a spring–mass model was developed to study the forces present in multi-row riveting. The model could accurately calculate the force–displacement curves during tensioning. The results show that when multiple rivet rows are used, higher stress concentrations cause the plate to first fracture at an outer rivet row with more rivets; therefore, the rivets should be arranged such that there are more rivets in the middle and fewer rivets on both sides. When the total rivet strength is greater than the remaining strength of the plate, the numbers of rivets and rivet rows have limited effects on the lap joint strength; however, this primarily affects the damaged form of the lap joint member. When the rivet spacing is less than 5d, the lap strength increases with increases in the rivet spacing, and when the rivet spacing is greater than 5d, the lap strength does not change significantly with increases in the rivet spacing. When the rivet edge distance is less than 3d, the lap joint strength increases with increases in the rivet edge distance, and when the rivet edge distance is greater than 3d, it has a limited effect on the lap joint strength. The rivet row spacing has no significant effect on the lap joint strength. The results of this study are valuable for improving the strengths of riveted structures in aircraft.
Application of Spectroscopy in Food Analysis: Volume II
Authors: Marini Federico , Biancolillo Alessandra
Published: May 2023

“Man is What He Eats”: food represents one of the fundamental needs of human beings, and, therefore, food analysis is a field of utmost importance [...]
Wearable-Based Intelligent Emotion Monitoring in Older Adults during Daily Life Activities
Authors: Gutierrez Maestro Eduardo , De Almeida Tiago Rodrigues , Schaffernicht Erik , Martinez Mozos Óscar
Published: May 2023

We present a system designed to monitor the well-being of older adults during their daily activities. To automatically detect and classify their emotional state, we collect physiological data through a wearable medical sensor. Ground truth data are obtained using a simple smartphone app that provides ecological momentary assessment (EMA), a method for repeatedly sampling people’s current experiences in real time in their natural environments. We are making the resulting dataset publicly available as a benchmark for future comparisons and methods. We are evaluating two feature selection methods to improve classification performance and proposing a feature set that augments and contrasts domain expert knowledge based on time-analysis features. The results demonstrate an improvement in classification accuracy when using the proposed feature selection methods. Furthermore, the feature set we present is better suited for predicting emotional states in a leave-one-day-out experimental setup, as it identifies more patterns.
Load-Bearing Characteristics of PHC Piles Constructed by the Inner Digging Method Based on Ultimate Load Testing and Numerical Simulation
Authors: Qu Yiwen , Zhang Yi , Wang Zhitian , Yang Dahai , Shi Jun
Published: May 2023

This paper studies the load-bearing characteristics of two prestressed high-strength concrete (PHC) pipe piles constructed by the medium mid-digging and hammering methods. The ultimate load tests and numerical simulations of the pipe piles constructed by both methods were carried out to analyze the ultimate lateral resistance, and ultimate resistance performance characteristics of the two pipe piles and the influence of the wall thickness of the pipe piles on the bearing performance. The test results show that the pipe pile constructed by the middle inner digging method has a higher pile quality. The single pile bearing capacity of the pipe pile constructed by the middle inner digging method is 50% higher than that of the common hammering method. The enlarged part of the pile end has an obvious effect on improving the bearing capacity. The settlement of the pipe pile constructed by the middle inner digging method is smaller than that of the hammering method. The large diameter pipe pile constructed by the middle inner digging method usually shows characteristics of the end-bearing pile. The resistance of the pile end accounts for 40–50% of the top load. The numerical simulation results agree with the field test and are compared and discussed. The simulation results show that when the bearing capacity of the pile is provided by the pile side frictional resistance, the influence of the pile wall thickness on the bearing capacity is insignificant. When the top pile load is close to the bearing capacity of the pipe pile, the influence of the pipe pile wall thickness on the bearing capacity is greater.
Digital Technologies’ Risks and Opportunities: Case Study of an RFID System
Authors: Naciri Lina , Gallab Maryam , Soulhi Aziz , Merzouk Safae , Di Nardo Mario
Published: May 2023

Smart technologies have been the subject of a growing interest for the past few years due to the growing market demand. They are believed to improve human life, existence, and companies’ performance. Considering the recent advances, X.0 concept has proven to be a mindset changing so that companies can now see that they can improve their competitiveness, ensure an innovative, sustainable and resilient environment, and smarten and develop their lean manufacturing tools. Nevertheless, if X.0 adoption is still not at its highest level, it is because of the relevant challenges and difficulties that occur during the implementation process. Within this scope, this paper aims, through a systematic literature review, to identify risks and opportunities of X.0 technologies to constitute a referential to be taken into consideration for a successful implementation. Results are validated by the modelling and simulation of an RFID system applied within the automotive industry, for which we identified risks and opportunities from one side and the system contribution in terms of smart Lean Manufacturing. From one hand, the value added of this paper, on the contrary of previous researches, is mainly regrouping risks and opportunities of most relevant digital technologies to conclude on those of X.0 revolution as a concept as described in following sections. From another hand, we were able to prove, through a real case study, that X.0 concept directly contribute in smartening and improving lean manufacturing principles.
Dynamics of Gene Expression Profiling and Identification of High-Risk Patients for Severe COVID-19
Authors: Rombauts Alexander , Bódalo Torruella Marta , Abelenda-Alonso Gabriela , Perera-Bel Júlia , Ferrer-Salvador Anna , Acedo-Terrades Ariadna , Gabarrós-Subirà Maria , Oriol Isabel , Gudiol Carlota , Nonell Lara , Carratalà Jordi
Published: May 2023

The clinical manifestations of SARS-CoV-2 infection vary widely, from asymptomatic infection to the development of acute respiratory distress syndrome (ARDS) and death. The host response elicited by SARS-CoV-2 plays a key role in determining the clinical outcome. We hypothesized that determining the dynamic whole blood transcriptomic profile of hospitalized adult COVID-19 patients and characterizing the subgroup that develops severe disease and ARDS would broaden our understanding of the heterogeneity in clinical outcomes. We recruited 60 hospitalized patients with RT-PCR-confirmed SARS-CoV-2 infection, among whom 19 developed ARDS. Peripheral blood was collected using PAXGene RNA tubes within 24 h of admission and on day 7. There were 2572 differently expressed genes in patients with ARDS at baseline and 1149 at day 7. We found a dysregulated inflammatory response in COVID-19 ARDS patients, with an increased expression of genes related to pro-inflammatory molecules and neutrophil and macrophage activation at admission, in addition to an immune regulation loss. This led, in turn, to a higher expression of genes related to reactive oxygen species, protein polyubiquitination, and metalloproteinases in the latter stages. Some of the most significant differences in gene expression found between patients with and without ARDS corresponded to long non-coding RNA involved in epigenetic control.
FDG and Non-FDG Radiopharmaceuticals for PET Imaging in Invasive Lobular Breast Carcinoma
Authors: Gilardi Laura , Airò Farulla Lighea Simona , Curigliano Giuseppe , Corso Giovanni , Leonardi Maria Cristina , Ceci Francesco
Published: May 2023

Invasive lobular cancer (ILC) is the second most frequent histological type of breast cancer (BC) and includes a heterogeneous spectrum of diseases with unique characteristics, especially the infiltrative growth pattern and metastatic spread. [18F]fluoro-2-deoxy-D-glucose positron emission tomography/computed tomography (FDG-PET/CT) is extensively used in oncology and BC patient evaluation. Its role in ILCs is considered suboptimal due to its low FDG avidity. Therefore, ILCs could benefit from molecular imaging with non-FDG tracers that target other specific pathways, contributing to precision medicine. This narrative review aims to summarize the current literature on the use of FDG-PET/CT in ILC and to discuss future opportunities given by the development of innovative non-FDG radiotracers.
Enhanced Proliferative and Osteogenic Potential of Periodontal Ligament Stromal Cells
Authors: Alves Laura , Machado Vanessa , Botelho João , Mendes José João , Cabral Joaquim M. S. , da Silva Cláudia L. , Carvalho Marta S.
Published: May 2023

Cell-based therapies using periodontal ligament stromal cells (PDLSC) for periodontal regeneration may represent an alternative source for mesenchymal stromal cells (MSC) to MSC derived from bone marrow (MSC(M)) and adipose tissue (MSC(AT)). We aimed to characterize the osteogenic/periodontal potential of PDLSC in comparison to MSC(M) and MSC(AT). PDLSC were obtained from surgically extracted healthy human third molars, while MSC(M) and MSC(AT) were obtained from a previously established cell bank. Flow cytometry, immunocytochemistry, and cell proliferation analyses provided cellular characteristics from each group. Cells from the three groups presented MSC-like morphology, MSC-related marker expression, and multilineage differentiation capacity (adipogenic, chondrogenic, and osteogenic). In this study, PDLSC expressed osteopontin, osteocalcin, and asporin, while MSC(M) and MSC(AT) did not. Of note, only PDLSC expressed CD146, a marker previously applied to identify PDLSC, and presented higher proliferative potential compared to MSC(M) and MSC(AT). Upon osteogenic induction, PDLSC exhibited higher calcium content and enhanced upregulation of osteogenic/periodontal genes compared to MSC(M) and MSC(AT), such as Runx2, Col1A1 and CEMP-1. However, the alkaline phosphatase activity of PDLSC did not increase. Our findings suggest that PDLSC might be a promising cell source for periodontal regeneration, presenting enhanced proliferative and osteogenic potential compared to MSC(M) and MSC(AT).
Efficient Purification of Polyhistidine-Tagged Recombinant Proteins Using Functionalized Corundum Particles
Authors: Völzke Jule L. , Smatty Sarah , Döring Sarah , Ewald Shireen , Oelze Marcus , Fratzke Franziska , Flemig Sabine , Konthur Zoltán , Weller Michael G.
Published: May 2023

Immobilized metal affinity chromatography (IMAC) is a popular and valuable method for the affinity purification of polyhistidine-tagged recombinant proteins. However, it often shows practical limitations, which might require cumbersome optimizations, additional polishing, and enrichment steps. Here, we present functionalized corundum particles for the efficient, economical, and fast purification of recombinant proteins in a column-free format. The corundum surface is first derivatized with the amino silane APTES, then EDTA dianhydride, and subsequently loaded with nickel ions. The Kaiser test, well known in solid-phase peptide synthesis, was used to monitor amino silanization and the reaction with EDTA dianhydride. In addition, ICP-MS was performed to quantify the metal-binding capacity. His-tagged protein A/G (PAG), mixed with bovine serum albumin (BSA), was used as a test system. The PAG binding capacity was around 3 mg protein per gram of corundum or 2.4 mg per 1 mL of corundum suspension. Cytoplasm obtained from different E. coli strains was examined as examples of a complex matrix. The imidazole concentration was varied in the loading and washing buffers. As expected, higher imidazole concentrations during loading are usually beneficial when higher purities are desired. Even when higher sample volumes, such as one liter, were used, recombinant protein down to a concentration of 1 µg/mL could be isolated selectively. Comparing the corundum material with standard Ni–NTA agarose beads indicated higher purities of proteins isolated using corundum. His6-MBP-mSA2, a fusion protein consisting of monomeric streptavidin and maltose-binding protein in the cytoplasm of E. coli, was purified successfully. To show that this method is also suitable for mammalian cell culture supernatants, purification of the SARS-CoV-2-S-RBD-His8 expressed in human Expi293F cells was performed. The material cost of the nickel-loaded corundum material (without regeneration) is estimated to be less than 30 cents for 1 g of functionalized support or 10 cents per milligram of isolated protein. Another advantage of the novel system is the corundum particles’ extremely high physical and chemical stability. The new material should be applicable in small laboratories and large-scale industrial applications. In summary, we could show that this new material is an efficient, robust, and cost-effective purification platform for the purification of His-tagged proteins, even in challenging, complex matrices and large sample volumes of low product concentration.
Differential Neural Correlates in the Prefrontal Cortex during a Delay Discounting Task in Healthy Adults: An fNIRS Study
Authors: Ikegami Masanaga , Sorama Michiko
Published: May 2023

The phenomenon of future rewards being devalued as a function of delay is referred to as delay discounting (DD). It is considered a measure of impulsivity, and steep DD characterizes psychiatric problems such as addictive disorders and attention deficit/hyperactivity disorder. This preliminarily study examined prefrontal hemodynamic activity using functional near-infrared spectroscopy (fNIRS) in healthy young adults performing a DD task. Prefrontal activity during a DD task with hypothetical monetary rewards was measured in 20 participants. A discounting rate (k-value) in the DD task was determined on the basis of a hyperbolic function. To validate the k-value, a DD questionnaire and the Barratt impulsiveness scale (BIS) were administered after fNIRS. The DD task induced a significant increase in oxygenated hemoglobin (oxy-Hb) concentration bilaterally in the frontal pole and dorsolateral prefrontal cortex (PFC) compared with a control task. Significant positive correlations were detected between left PFC activity and discounting parameters. Right frontal pole activity, however, showed significantly negative correlation with motor impulsivity as a BIS subscore. These results suggest that left and right PFCs have differential contributions when performing the DD task. The present findings suggest the idea that fNIRS measurement of prefrontal hemodynamic activity can be useful for understanding the neural mechanisms underlying DD and is applicable for assessing PFC function among psychiatric patients with impulsivity-related problems.
The Evolution of Therapies Targeting Bruton Tyrosine Kinase for the Treatment of Chronic Lymphocytic Leukaemia: Future Perspectives
Authors: Eyre Toby A. , Riches John C.
Published: May 2023

The development of inhibitors of Bruton tyrosine kinase (BTK) and B-cell lymphoma 2 (BCL2) has resulted in a paradigm shift in the treatment of chronic lymphocytic leukaemia (CLL) over the last decade. Observations regarding the importance of B-cell receptor signalling for the survival and proliferation of CLL cells led to the development of the first-in-class BTK inhibitor (BTKi), ibrutinib, for the treatment of CLL. Despite being better tolerated than chemoimmunotherapy, ibrutinib does have side effects, some of which are due to the off-target inhibition of kinases other than BTK. As a result, more specific inhibitors of BTK were developed, such as acalabrutinib and zanubrutinib, which have demonstrated equivalent/enhanced efficacy and improved tolerability in large randomized clinical trials. Despite the increased specificity for BTK, side effects and treatment resistance remain therapeutic challenges. As these drugs all bind covalently to BTK, an alternative approach was to develop noncovalent inhibitors of BTK, including pirtobrutinib and nemtabrutinib. The alternative mechanisms of BTK-binding of these agents has the potential to overcome resistance mutations, something that has been borne out in early clinical trial data. A further step in the clinical development of BTK inhibition has been the introduction of BTK degraders, which remove BTK by ubiquitination and proteasomal degradation, in marked contrast to BTK inhibition. This article will review the evolution of BTK inhibition for CLL and offer future perspectives on the sequencing of an increasing number of different agents, and how this may be impacted on by mutations in BTK itself and other kinases.
A Mathematical Model for Predicting Patient Responses to Combined Radiotherapy with CTLA-4 Immune Checkpoint Inhibitors
Authors: Kim Yongjin , Choe Bo-Young , Suh Tae Suk , Sung Wonmo
Published: May 2023

The purpose of this study was to develop a cell–cell interaction model that could predict a tumor’s response to radiotherapy (RT) combined with CTLA-4 immune checkpoint inhibition (ICI) in patients with hepatocellular carcinoma (HCC). The previously developed model was extended by adding a new term representing tremelimumab, an inhibitor of CTLA-4. The distribution of the new immune activation term was derived from the results of a clinical trial for tremelimumab monotherapy (NCT01008358). The proposed model successfully reproduced longitudinal tumor diameter changes in HCC patients treated with tremelimumab (complete response = 0%, partial response = 17.6%, stable disease = 58.8%, and progressive disease = 23.6%). For the non-irradiated tumor control group, adding ICI to RT increased the clinical benefit rate from 8% to 32%. The simulation predicts that it is beneficial to start CTLA-4 blockade before RT in terms of treatment sequences. We developed a mathematical model that can predict the response of patients to the combined CTLA-4 blockade with radiation therapy. We anticipate that the developed model will be helpful for designing clinical trials with the ultimate aim of maximizing the efficacy of ICI-RT combination therapy.
Weed Control and Selectivity of Four Herbicides Applied in Pre-Emergence on Two Sunflower Cultivars
Authors: dos Santos Elielton Germano , Inoue Miriam Hiroko , Guimarães Ana Carolina Dias , Bastos Jennifer Stefany Queiroz , Mendes Kassio Ferreira
Published: May 2023

The sunflower (Helianthus annuus L.) is an oleaginous plant that shows high suitability for cultivation in Brazil. However, the performance of the crop is influenced by factors such as weed interference, mainly because of the few selective herbicide options. Thus, the objective of this study was to evaluate weed control and selectivity of four pre-emergent herbicides applied to two sunflower cultivars in sandy and clayey soils. Two field experiments were conducted in a randomized block design with a 2 × 6 factorial scheme, two cultivars (CF 101 and M 734) and six treatments with S-metolachlor (1920 g a.i. ha−1), sulfentrazone (150 g a.i. ha−1), flumioxazin (40 g a.i. ha−1), and trifluralin (1780 g a.i. ha−1) and two controls, one with weeding and the other without weeding and without herbicide application, in soils with contrasting textures (sandy and clayey). No injury symptoms were observed in both cultivars, regardless of soil texture. The cultivar M 734 exhibited the highest values of the agronomic parameters evaluated. The treatment with flumioxazin provided control of more than 90% of the weeds in clayey soil and more than 64% in sandy soil. The yield of both cultivars was lower in the sandy soil. Thus, all herbicide treatments were selective for both cultivars tested, since they did not interfere with sunflower grain yield.
Butterfly Assemblages Differ among Vegetation Types in Southern Amazonia
Authors: Mota Luísa L. , Santos Jessie P. , Willmott Keith R. , Freitas André V. L.
Published: May 2023

Environmental heterogeneity is considered an important factor supporting the evolution and maintenance of biodiversity. At small scales, such heterogeneity is thought to promote species co-existence through an increase in niche opportunities. Amazonia, the largest and most biodiverse rainforest in the world, presents a large number of vegetation types within its territory. Here, we tested the hypothesis that butterfly assemblages differ among five vegetation types at a small scale (less than 1 km2) in a region of Southern Amazonia. The vegetation types studied were forest gap, terra firme, igapó, semi-deciduous forest, and bamboo forest. The richest and most abundant community was in forest gap; igapó was the least rich, but held the second most abundant community and the only one with nine indicator species instead of two or three. Assemblage composition differed among all vegetation types, with the exception of forest gap and bamboo forest. Different light levels, temperatures, humidity, and host plant availability among vegetation types are likely relevant factors influencing these butterfly assemblages. The results suggest that the presence of various vegetation types in the region promotes the coexistence of butterfly species, and that specific threats to each vegetation type should be addressed to conserve the region’s biodiversity.
Performance Evaluation of SARS-CoV-2 Viral Transport Medium Produced by Bangladesh Reference Institute for Chemical Measurements
Authors: Razu Mamudul Hasan , Monir Bayzid Bin , Moniruzzaman Md. , Sarkar Sawgotom , Akhter Sonia , Kamal Sabiha , Hasan Md. Abu , Afroze Mirola , Imam Khandaker Md. Sharif Uddin , Khan Mala
Published: May 2023

A viral transport medium (VTM) was developed following the Centers for Disease Control and Prevention, USA (US-CDC) standard operating procedure (SOP) DSR-052-05 with necessary improvisation and was used for storing coronavirus disease 2019 (COVID-19) swab specimens. Considering Bangladesh’s supply chain and storage conditions, improvisation was essential for extending sample storage time while retaining efficiency. In-house VTM was produced using Hank’s balanced salt solution (HBSS) supplemented with 1% bovine serum albumin V (BSA), 0.5 µg /mL of gentamicin sulfate, and 100 µg/mL of fluconazole. The produced VTM composition, quality, sterility, specificity, and efficiency were verified in-house and through an independent contract research organization (CRO). An accelerated stability study projected that under the recommended temperature (4 °C), it would remain stable for four months and preserve samples for over a month. The real-time reverse transcriptase–polymerase chain reaction (rRT-PCR) test detected the targeted N gene and ORF1ab gene from the VTM stored samples. Our VTM is equally as effective as the Sansure Biotech VTM in keeping SARS-CoV-2 RNA specimens detectable in rRT-PCR (100% sensitivity and specificity in random and blinded samples). In conclusion, the BRiCM VTM will make the battle against pandemics easier by effectively collecting and storing nasopharyngeal and oropharyngeal swabs for COVID-19 detection.
UAVs for Business Adoptions in Smart City Environments: Inventory Management System
Authors: Salazar Franklin , Martínez-García María Sofía , de Castro Angel , Chávez-Fuentes Carla , Cazorla María , Ureña-Aguirre Jeanette del Pilar , Altamirano Santiago
Published: May 2023

Unmanned aerial vehicle (UAV)-assisted inventory management, used for inventory control within an enterprise with large warehousing and manufacturing systems, is currently common practice. There are many applications for UAVs within an enterprise, but they are generally used in tasks that have high risk connotations to perform activities quickly, such as in the cases of large enterprises that generally require inventory of many products quickly and efficiently connected in a smart city environment. Having an up-to-date inventory as quickly as possible in a smart city environment allows users to have the necessary information to verify the availability of products, reducing waiting times. The use of UAVs systems compared to conventional systems, such as unmanned ground vehicles, has several advantages, including the fact that UAVs have their own cameras and can move independently within the warehouse. This paper proposes the development of a Supervisory Control and Data Acquisition system (SCADA) for the inventory management of the company DHL, using drones. This study evaluates the OPC communication protocols for the virtual environment and the 232 serial connection protocol for the real environment, using LabView and Factory I/O programs. For the development of the system, the use of a drone was evaluated for the recognition of the availability of merchandise with the use of infrared technology incorporated in the drone by means of an Arduino. The appropriate use of communication protocols allowed the necessary information to be obtained for the communication of the LabView and Factory I/O environment in which a virtual environment based on the DHL inventory system is available. The system allowed the development of a SCADA system for the management of inventory monitoring in real time of the availability of goods in the virtual environment, allowing to obtain the necessary information for its integration in a smart city environment.
Experimental Study on Spray Cooling Heat Transfer of LN2 for a Large Area
Authors: Zhao Jinhong , Guo Yanming , Ai Qing , Gong Jiaming , Shuai Yong
Published: May 2023

Spray cooling has been considered one of the most promising thermal control methods of high-heat flux devices. Most of the spray cooling research focuses on electronic components as the main application object to achieve higher heat dissipation heat flow in ambient temperature regions for small areas. Water is the most common cooling medium. This paper investigates the application of spray cooling thermal control over large areas. In this study, the heat-transfer characteristics of liquid nitrogen (LN2) for large areas was investigated by conducting experiments. The test surface is 500 mm × 500 mm, which was cooled by a nine-nozzle array. The spray nozzles used in the experiment were conical nozzles with an orifice diameter of 1.6 mm, a spray angle of 120°, and a spray height of 42 mm. Liquid nitrogen was forcefully ejected from nozzles by the high pressure of a liquid storage tank to cool the test surface. According to the cooled surfaces, spray directions, and spray pressures, three groups of experiments were conducted. The results showed that the smooth flat surface has the best heat-transfer performance in three kinds of surface structures, which are macro surface, porous surface, and smooth flat surface. The heat-transfer coefficient varied by ±20% with different spray directions, and the surface heat-transfer coefficient increased linearly with increasing spray pressure. Most of the spray cooling research focuses on heat dissipation in the ambient temperature region for equipment over small areas. The results can benefit thermal control application in various fields. The research in this paper can provide a reference for the application of large-area spray cooling, and the application areas mainly include metal manufacturing processing cooling, aircraft skin infrared radiation characteristics modulation, and laser weapon equipment cooling.
The Role of Direct Air Capture in EU’s Decarbonisation and Associated Carbon Intensity for Synthetic Fuels Production
Authors: Gonzalez Sanchez Rocio , Chatzipanagi Anatoli , Kakoulaki Georgia , Buffi Marco , Szabo Sandor
Published: May 2023

Direct air capture (DAC) is considered one of the mitigation strategies in most of the future scenarios trying to limit global temperature to 1.5 °C. Given the high expectations placed on DAC for future decarbonisation, this study presents an extensive review of DAC technologies, exploring a number of techno-economic aspects, including an updated collection of the current and planned DAC projects around the world. A dedicated analysis focused on the production of synthetic methane, methanol, and diesel from DAC and electrolytic hydrogen in the European Union (EU) is also performed, where the carbon footprint is analysed for different scenarios and energy sources. The results show that the maximum grid carbon intensity to obtain negative emissions with DAC is estimated at 468 gCO2e/kWh, which is compliant with most of the EU countries’ current grid mix. Using only photovoltaics (PV) and wind, negative emissions of at least −0.81 tCO2e/tCO2 captured can be achieved. The maximum grid intensities allowing a reduction of the synthetic fuels carbon footprint compared with their fossil-fuels counterparts range between 96 and 151 gCO2e/kWh. However, to comply with the Renewable Energy Directive II (REDII) sustainability criteria to produce renewable fuels of non-biological origin, the maximum stays between 30.2 to 38.8 gCO2e/kWh. Only when using PV and wind is the EU average able to comply with the REDII threshold for all scenarios and fuels, with fuel emissions ranging from 19.3 to 25.8 gCO2e/MJ. These results highlight the importance of using renewable energies for the production of synthetic fuels compliant with the EU regulations that can help reduce emissions from difficult-to-decarbonise sectors.
Species Composition and Seasonal Abundance of Predatory Mites (Acari: Phytoseiidae) Inhabiting Aesculus hippocastanum (Sapindaceae)
Authors: Kopačka Michal , Zemek Rostislav
Published: May 2023

Species of the family Phytoseiidae (Acari: Mesostigmata) inhabit a wide range of herbs, shrubs, and trees. Horse chestnut, Aesculus hippocastanum, is an important ornamental tree in Europe and, in addition to its importance for pollinators, it can serve as a host plant of these predatory mites. Little is, however, known about the factors affecting spatiotemporal variability in the density of phytoseiids on A. hippocastanum in an urban environment. We therefore assessed the species composition and the spatial and seasonal variability in the abundance of Phytoseiidae species in the city of České Budějovice, South Bohemia, Czech Republic. Compound-leaf samples were randomly collected from horse chestnut tree branches at eight sites, five times during the vegetation season in 2013. The mites were collected by washing technique and mounted on slides for identification. In total, 13,903 specimens of phytoseiid mites were found, and eight species were identified: Amblyseius andersoni, Euseius finlandicus, Kampimodromus aberrans, Neoseiulella tiliarum, Phytoseius macropilis, Paraseiulus talbii, Paraseiulus triporus, and Typhlodromus (Typhlodromus) pyri. The predominant species was E. finlandicus (96.25%). The mean number of mites per compound leaf was 2.53, 10.40, 23.54, 11.59, and 9.27 on the sampling dates in each month between May and September, respectively. The results further revealed that the mite abundance varied significantly among sampling sites and that it was negatively related to percentage of greenery area, intensity of greenery care, distance to water body, and density and age of horse chestnut trees, while it was positively related to air pollution index. The importance of leaf micromorphology for the attractiveness of A. hippocastanum to Phytoseiidae is discussed.
Metal Oxide Nanoparticles in Food Packaging and Their Influence on Human Health
Authors: Stuparu-Cretu Mariana , Braniste Gheorghe , Necula Gina-Aurora , Stanciu Silvius , Stoica Dimitrie , Stoica Maricica
Published: May 2023

It is a matter of common knowledge in the literature that engineered metal oxide nanoparticles have properties that are efficient for the design of innovative food/beverage packages. Although nanopackages have many benefits, there are circumstances when these materials are able to release nanoparticles into the food/beverage matrix. Once dispersed into food, engineered metal oxide nanoparticles travel through the gastrointestinal tract and subsequently enter human cells, where they display various behaviors influencing human health or wellbeing. This review article provides an insight into the antimicrobial mechanisms of metal oxide nanoparticles as essential for their benefits in food/beverage packaging and provides a discussion on the oral route of these nanoparticles from nanopackages to the human body. This contribution also highlights the potential toxicity of metal oxide nanoparticles for human health. The fact that only a small number of studies address the issue of food packaging based on engineered metal oxide nanoparticles should be particularly noted.
Optimizing Delivery of Therapeutic Growth Factors for Bone and Cartilage Regeneration
Authors: Takematsu Eri , Murphy Matthew , Hou Sophia , Steininger Holly , Alam Alina , Ambrosi Thomas H. , Chan Charles K. F.
Published: May 2023

Bone- and cartilage-related diseases, such as osteoporosis and osteoarthritis, affect millions of people worldwide, impairing their quality of life and increasing mortality. Osteoporosis significantly increases the bone fracture risk of the spine, hip, and wrist. For successful fracture treatment and to facilitate proper healing in the most complicated cases, one of the most promising methods is to deliver a therapeutic protein to accelerate bone regeneration. Similarly, in the setting of osteoarthritis, where degraded cartilage does not regenerate, therapeutic proteins hold great promise to promote new cartilage formation. For both osteoporosis and osteoarthritis treatments, targeted delivery of therapeutic growth factors, with the aid of hydrogels, to bone and cartilage is a key to advance the field of regenerative medicine. In this review article, we propose five important aspects of therapeutic growth factor delivery for bone and cartilage regeneration: (1) protection of protein growth factors from physical and enzymatic degradation, (2) targeted growth factor delivery, (3) controlling GF release kinetics, (4) long-term stability of regenerated tissues, and (5) osteoimmunomodulatory effects of therapeutic growth factors and carriers/scaffolds.
Pattern of Antibiotic Use among Hospitalized Patients at a Level One Multidisciplinary Care Hospital
Authors: Hodoșan Viviana , Daina Lucia Georgeta , Zaha Dana Carmen , Cotrău Petru , Vladu Adriana , Dorobanțu Florica Ramona , Negrău Marcel Ovidiu , Babeș Elena Emilia , Babeș Victor Vlad , Daina Cristian Marius
Published: May 2023

Background: Antimicrobial resistance is one of the world’s most serious health issues. Antibiotic resistance, excessive drug expense, and an increased risk of adverse reactions are all common outcomes of incorrect antibiotic prescribing. The goal of this study was to evaluate the prevalence of antibiotic prescriptions for inpatients to find areas for improvement. Methods: A retrospective study at Emergency Clinical County Hospital of Oradea, Romania was performed for five years between 2017 and 2021. Data was collected using medical records of the patients and reports from the pharmacy. Antibiotic consumption was expressed as DDD/100 BD according to the World Health Organization (WHO) by antibiotics, classes, and AWaRe classification. Results: The prevalence of antibiotic prescription was 53.8% during five years evaluated with a significant increase in 2021. A total of 13,677.42 DDD/100 BD antibiotics were prescribed, especially for surgical and medical prophylaxes. The most prescribed antibiotics were ceftriaxone, followed by metronidazole, and cefuroxime but there were some differences between years and wards. The most frequent antibiotic classes prescribed were cephalosporins (43.73%). The use of Watch Group antibiotics was high in all wards (59.69%). Conclusions: The prevalence of antibiotic use was high with cephalosporins being the most prescribed antibiotics. As a result, interventions are required.
Evaluating the Impact of COVID-19 on Hospital Profit Compensation Activities: A Difference-in-Differences Event Study Analysis in China
Authors: Shen Chi , Cao Dan , Deng Qiwei , Lai Sha , Liu Guanping , Yang Liu , Zhu Zhonghai , Zhou Zhongliang
Published: May 2023

The impact of the 2019 coronavirus disease (COVID-19) pandemic is still being revealed, and little is known about the effect of COVID-19-induced outpatient and inpatient losses on hospital operations in many counties. Hence, we aimed to explore whether hospitals adopted profit compensation activities after the 2020 first-wave outbreak of COVID-19 in China. A total of 2,616,589 hospitalization records from 2018, 2019, and 2020 were extracted from 36 tertiary hospitals in a western province in China; we applied a difference-in-differences event study design to estimate the dynamic effect of COVID-19 on hospitalized patients’ total expenses before and after the last confirmed case. We found that average total expenses for each patient increased by 8.7% to 16.7% in the first 25 weeks after the city reopened and hospital admissions returned to normal. Our findings emphasize that the increase in total inpatient expenses was mainly covered by claiming expenses from health insurance and was largely driven by an increase in the expenses for laboratory tests and medical consumables. Our study documents that there were profit compensation activities in hospitals after the 2020 first-wave outbreak of COVID-19 in China, which was driven by the loss of hospitalization admissions during this wave outbreak.
Multifactorial Study on the Impact of Educational Level, Employment Status, and the Need for Extraordinary Care on the Economic Impact of Cancer Patients
Authors: Martín Alberto García , Fernández Rodríguez Eduardo J. , Sánchez Gómez Celia , Galve M. Isabel Rihuete
Published: May 2023

Cancer is one of the major socio-health problems in the world. The level of education, the profession and/or employment status of the patient and the family can influence the amount of household income, the additional expenditure, and the possible socio-economic impact of the disease. The main objective of the study is to analyse and evaluate the influence of the level of education, the employment status of the patient, and the need for care and how these factors influence the additional expenditure and the possible socio-economic impact. Methods: descriptive cross-sectional randomised observational epidemiological study without replacement at the Hospital Universitario de Salamanca (CAUSA). Results: total sample (n = 365) comprised 53.2% of patients with no education or primary education, 25.8% with secondary education and 21.1% with higher education. Overall, 36.4% of patients were employed, 10.1% were self-employed, 53.0% were not employed, and 38.9% were experiencing other conditions. Significant statistics were found for educational level, employment status of the patient and main caregivers in terms of financial expenditure. Conclusions: Oncology patients with more education spend more on home help and/or accompanying the patient and transfers to the hospital for treatment. Higher incomes are not synonymous with higher expenditure in the sample. The patient’s main caregivers are a fundamental pillar of the patient’s household income and additional spending on orthopaedic material.
Effects of a Nudging Cue Targeting Food Choice in a University Cafeteria: A Field Study
Authors: Kawa Christine , Ianiro-Dahm Patrizia M. , Nijhuis Jan F. H. , Gijselaers Wim H.
Published: May 2023

Many students approaching adulthood often choose high-calorie food products. Concurrently, health interventions applied during this life phase can potentially lead to a healthier lifestyle. Nudge health interventions in experimental cafeteria settings have been found to improve eating behavior effectively, yet research in real-world settings is lacking. Accepting nudges as health interventions impacts nudge effectiveness. The present study applies a pretest–posttest design for a period of three consecutive weeks (no nudge, nudge, no nudge), testing the effectiveness of the so-called Giacometti cue on the number of calories purchased in a real-world cafeteria. Students were exposed to the nudge during the intervention week when entering the cafeteria and when choosing their meals. After purchasing a meal, their choice was recorded, and they completed a questionnaire. The Giacometti cue immediately reduced the number of calories purchased (comparing weeks one and two). After nudge removal, an effect was identified, increasing the number of calories purchased (comparing weeks two and three). Contrary to expectations, higher nudge acceptance resulted in more calories purchased. Neither awareness of the nudge’s presence when buying food nor the interaction between acceptance and awareness played a role. We explore potential explanations for the Giacometti cue’s effects.
Non-Classical Aspects of Obesity Pathogenesis and Their Relative Clinical Importance for Obesity Treatment
Authors: Samakidou Georgia E. , Koliaki Chrysi C. , Liberopoulos Evangelos N. , Katsilambros Nikolaos L.
Published: May 2023

Obesity is a chronic disease and a major public health problem due to its association with non-communicable diseases and all-cause mortality. An increased energy intake and decreased physical activity have been long recognized as the classical parameters that contribute to the development of obesity. However, several other, non-classical factors have also been associated with obesity through various complex mechanisms. Some of them are diet related, such as diet quality, dietary habits and speed of eating. Other factors are non-dietary, such as endocrine-disrupting chemicals, sleep quality and quantity, psychotropic medications and light at night. The scope of the present narrative review is to address these non-classical factors that are implicated in the pathogenesis of obesity, to clarify their potential role in the management of obesity and, where possible, to provide some practical clinical recommendations.
DBT-PAHSE Intervention for Reduce Emotion Dysregulation and Suicide Behavior in Mexican Early Adolescents: A Longitudinal Study
Authors: Hermosillo-de-la-Torre Alicia E. , Arteaga-de-Luna Stephania M. , Arenas-Landgrave Paulina , González-Forteza Catalina , Acevedo-Rojas Denise L. , Martínez Kalina I. , Rivera-Heredia María E.
Published: May 2023

This study aimed to evaluate the effect of time on suicidal behavior, associated risk factors, and protective factors in early Mexican adolescents. Method: With a two-year longitudinal design, which included 18 of 34 adolescents who had previously participated in a DBT skills training program (DBT-PAHSE). The study evaluated ideation, suicide attempt, depression, emotional dysregulation, and psychological resources. Results: We observed differentiating significant differences over time in emotional dysregulation (F = 2.36 p = 0.04, η2= 0.12, β = 0.72), affective resources (F = 3.94, p = 0.01, η2 = 0.18, β = 0.82), and suicidal ideation. (F = 2.55, p = 0.03, η2= 0.13, β = 0.77). In conclusion, the DBT-PAHSE program prevented deaths by suicide. It showed a reduction in emotional dysregulation up to two years after the end of treatment and maintained an increase in emotional and social resources. However, improvements are required to reduce depression over time and strengthen psychological resources.
Trends in the Prevalence of Diabetes Mellitus in Pregnancy during the Past Two Decades in Northern Thailand
Authors: Jatavan Phudit , Luewan Suchaya , Sirilert Sirinart , Tongsong Theera
Published: May 2023

Background: The prevalence of gestational diabetes mellitus (GDM) and pre-gestational diabetes mellitus (PDM) has increased dramatically in the past decade in all ethnic groups. The prevalence also varies markedly among different ethnic groups. Each ethnic group must have its own data about GDM/PDM for improvement in women’s health care. We conducted this study with the main objective of assessing recent trends in the prevalence of PDM/GDM among pregnant women in the northern part of Thailand during the past two decades. The secondary objective is to identify the risk factors influencing the prevalence of DM in pregnancies. Patients and Methods: The maternal–fetal medicine database was accessed to retrieve consecutive obstetric records of women who gave birth in Chiang Mai University Hospital, Thailand, from January 2003 to December 2022. This is a 20-year study period of the same protocol of GDM screening policy, using the 50 g glucose challenge test as a screening test for the average risk group and the 100 g OGTT as a diagnostic test. The women were categorized into GDM, PDM and non-DM groups. Trends or percentage changes in the prevalence of GDM/PDM during the study period were evaluated. Risk factors related to GDM/PDM were identified. Results: Among 37,027 women who gave birth during the study period, the prevalence of DM in pregnancy was 11.4% (4223 cases), including 214 cases of PDM (0.6%) and 4009 cases of GDM (10.8%). The prevalence of PDM significantly increased from 0.3% in 2003 to 1.5% in 2022; also, the prevalence of GDM significantly increased, dramatically, from 3.4% in 2003 to 22.0% in 2022. The prevalence of GDM increased in recent years in all age groups (adolescent, reproductive and elderly groups), while that of PDM did not significantly change in the adolescent group during the study period. Maternal age and pre-pregnancy BMI significantly increased in the more recent years. Independent factors significantly associated with the prevalence of PDM/GDM include maternal age, pre-pregnancy BMI, higher socio-economic status, and urban areas of residence. Recent time is still an independent risk factor after adjustment for other known factors. Conclusions: Relatively, GDM and PDM are highly prevalent in the northern part of Thailand, and their prevalence continuously increased during the past two decades. The trend of increased prevalence was evident in all age groups. Increasing maternal age and pre-pregnancy BMI mainly contributed to the increase in the prevalence of GDM and PDM in recent years. Recent time is still an independent risk factor after adjustment for other known factors, indicating that some other unexplained risk factors are associated with the increase in prevalence of DM in recent years, possibly the increase in sedentary lifestyle. Modification of lifestyle, especially reducing pre-pregnancy BMI among reproductive women, may reduce the prevalence of DM in pregnancy.