bluuebunny/crossref_metadata_2025 huggingface dataset:
Noncompliance with Hypertension Treatment and Related Factors among Kumamoto Earthquake Victims Who Experienced the COVID-19 Pandemic during Postearthquake Recovery Period
Authors: Ide-Okochi Ayako , He Mu , Murayama Hiroshi , Samiso Tomonori , Yoshinaga Naoki
Published: March 2023

Survivors of the Kumamoto earthquake of 2016 experienced the coronavirus disease (COVID-19) outbreak while carrying additional burdens that might bring inadequate coping. This cross-sectional survey aimed to identify untreated and interrupted consultations among those with hypertension and related factors and to identify the disaster’s long-term effects. Of the 19,212 earthquake survivors who had moved to permanent housing, 7367 (4196 women and 3171 men, mean age 61.8 ± 17.3 years) completed a self-administered questionnaire. The prevalence of hypertension was 41.4%. The results of the logistic regression analysis with the significant independent variables in the bivariate analysis were: reduced income due to COVID-19 (AOR = 3.23, 95%CI = 2.27–4.58) and poor self-rated health (AOR = 2.49, 95%CI = 1.72–3.61) were associated with a risk of untreated or discontinued treatment. Moreover, living in rental, public or restoration public housing was also significantly associated with a higher risk of hypertension noncompliance (AOR = 1.92, 95%CI = 1.20–3.07; AOR = 2.47, 95%CI = 1.38–4.42; AOR = 4.12, 95%CI = 1.14–14.90). These results suggest that changes due to COVID-19, the extent of self-rated health and the type of permanent housing influence the hypertension consulting behaviour of earthquake survivors during recovery. It is crucial to implement long-term public support for the mental health, income and housing concerns of the survivors.
Pandemic Education—Insights into Teachers’ Perceptions of Hygiene Measures in Schools Due to COVID-19
Authors: Woltran Flora , Lindner Katharina-Theresa , Schwab Susanne
Published: March 2023

To prevent the spread of Coronavirus (COVID-19) and protect the health of school staff and students, Austrian education policymakers introduced several hygiene measures that posed new challenges for teachers. The current paper focuses on teachers’ perceptions of hygiene measures in schools during the 2021–2022 school year. In Study 1, 1372 Austrian teachers participated in an online survey at the end of 2021. In Study 2, five teachers participated in an in-depth qualitative interview study. The quantitative results show that half the teachers felt a strong burden from the COVID-19 tests, but that the tests worked better when teachers had more teaching experience. Elementary and secondary school teachers, unlike special education teachers, had fewer problems implementing COVID-19 testing. The qualitative results suggest that teachers needed an acclimatization period for previously unfamiliar tasks, such as COVID-19 testing, to become accustomed to this newly implemented measure. Additionally, wearing facemasks was only evaluated positively in the context of self-serving strategies, while the protection of student health was not considered. In summary, the current study calls attention to the particular vulnerability of teachers and provides insights into the reality of schools in times of crisis that could be particularly helpful to education policymakers.
The Evaluation of Geographical Health Facilities Structure in Makassar City, Indonesia
Authors: Yudono Adipandang , Afrianto Firman , Hariyanto Annisa Dira
Published: March 2023

Cities across the world, during the last period, have been shocked by the outbreak of the COVID-19 pandemic. The world of planning has since persevered in providing a response, in terms of how to anticipate this outbreak in the future. Various kinds of concepts have been issued, with various views and points of view. However, one of the needs for this planning is an appropriate evaluation of the geographic structure of existing health facilities, in order to properly provide consideration for future urban planning. This study attempts to provide an integrated model of how to evaluate the geographic structure of health facilities with a case study in Makassar City, Indonesia. By combining big data and spatial analysis, it is expected that it will find patterns and directions for acceptable health facilities planning.
Adult and Larval Tracheal Systems Exhibit Different Molecular Architectures in Drosophila
Authors: Bossen Judith , Prange Ruben , Kühle Jan-Philip , Künzel Sven , Niu Xiao , Hammel Jörg U. , Krieger Laura , Knop Mirjam , Ehrhardt Birte , Uliczka Karin , Krauss-Etschmann Susanne , Roeder Thomas
Published: March 2023

Knowing the molecular makeup of an organ system is required for its in-depth understanding. We analyzed the molecular repertoire of the adult tracheal system of the fruit fly Drosophila melanogaster using transcriptome studies to advance our knowledge of the adult insect tracheal system. Comparing this to the larval tracheal system revealed several major differences that likely influence organ function. During the transition from larval to adult tracheal system, a shift in the expression of genes responsible for the formation of cuticular structure occurs. This change in transcript composition manifests in the physical properties of cuticular structures of the adult trachea. Enhanced tonic activation of the immune system is observed in the adult trachea, which encompasses the increased expression of antimicrobial peptides. In addition, modulatory processes are conspicuous, in this case mainly by the increased expression of G protein-coupled receptors in the adult trachea. Finally, all components of a peripheral circadian clock are present in the adult tracheal system, which is not the case in the larval tracheal system. Comparative analysis of driver lines targeting the adult tracheal system revealed that even the canonical tracheal driver line breathless (btl)-Gal4 is not able to target all parts of the adult tracheal system. Here, we have uncovered a specific transcriptome pattern of the adult tracheal system and provide this dataset as a basis for further analyses of the adult insect tracheal system.
The Blood Microbiome and Health: Current Evidence, Controversies, and Challenges
Authors: Cheng Hong Sheng , Tan Sin Pei , Wong David Meng Kit , Koo Wei Ling Yolanda , Wong Sunny Hei , Tan Nguan Soon
Published: March 2023

Blood is conventionally thought to be sterile. However, emerging evidence on the blood microbiome has started to challenge this notion. Recent reports have revealed the presence of genetic materials of microbes or pathogens in the blood circulation, leading to the conceptualization of a blood microbiome that is vital for physical wellbeing. Dysbiosis of the blood microbial profile has been implicated in a wide range of health conditions. Our review aims to consolidate recent findings about the blood microbiome in human health and to highlight the existing controversies, prospects, and challenges around this topic. Current evidence does not seem to support the presence of a core healthy blood microbiome. Common microbial taxa have been identified in some diseases, for instance, Legionella and Devosia in kidney impairment, Bacteroides in cirrhosis, Escherichia/Shigella and Staphylococcus in inflammatory diseases, and Janthinobacterium in mood disorders. While the presence of culturable blood microbes remains debatable, their genetic materials in the blood could potentially be exploited to improve precision medicine for cancers, pregnancy-related complications, and asthma by augmenting patient stratification. Key controversies in blood microbiome research are the susceptibility of low-biomass samples to exogenous contamination and undetermined microbial viability from NGS-based microbial profiling, however, ongoing initiatives are attempting to mitigate these issues. We also envisage future blood microbiome research to adopt more robust and standardized approaches, to delve into the origins of these multibiome genetic materials and to focus on host–microbe interactions through the elaboration of causative and mechanistic relationships with the aid of more accurate and powerful analytical tools.
Circadian Oscillations in Skin and Their Interconnection with the Cycle of Life
Authors: Salazar Andrew , von Hagen Jörg
Published: March 2023

Periodically oscillating biological processes, such as circadian rhythms, are carefully concerted events that are only beginning to be understood in the context of tissue pathology and organismal health, as well as the molecular mechanisms underlying these interactions. Recent reports indicate that light can independently entrain peripheral circadian clocks, challenging the currently prevalent hierarchical model. Despite the recent progress that has been made, a comprehensive overview of these periodic processes in skin is lacking in the literature. In this review, molecular circadian clock machinery and the factors that govern it have been highlighted. Circadian rhythm is closely linked to immunological processes and skin homeostasis, and its desynchrony can be linked to the perturbation of the skin. The interplay between circadian rhythm and annual, seasonal oscillations, as well as the impact of these periodic events on the skin, is described. Finally, the changes that occur in the skin over a lifespan are presented. This work encourages further research into the oscillating biological processes occurring in the skin and lays the foundation for future strategies to combat the adverse effects of desynchrony, which would likely have implications in other tissues influenced by periodic oscillatory processes.
Integrative Analysis of the Ethanol Tolerance of Saccharomyces cerevisiae
Authors: Wolf Ivan Rodrigo , Marques Lucas Farinazzo , de Almeida Lauana Fogaça , Lázari Lucas Cardoso , de Moraes Leonardo Nazário , Cardoso Luiz Henrique , Alves Camila Cristina de Oliveira , Nakajima Rafael Takahiro , Schnepper Amanda Piveta , Golim Marjorie de Assis , Cataldi Thais Regiani , Nijland Jeroen G. , Pinto Camila Moreira , Fioretto Matheus Naia , Almeida Rodrigo Oliveira , Driessen Arnold J. M. , Simōes Rafael Plana , Labate Mônica Veneziano , Grotto Rejane Maria Tommasini , Labate Carlos Alberto , Fernandes Junior Ary , Justulin Luis Antonio , Coan Rafael Luiz Buogo , Ramos Érica , Furtado Fabiana Barcelos , Martins Cesar , Valente Guilherme Targino
Published: March 2023

Ethanol (EtOH) alters many cellular processes in yeast. An integrated view of different EtOH-tolerant phenotypes and their long noncoding RNAs (lncRNAs) is not yet available. Here, large-scale data integration showed the core EtOH-responsive pathways, lncRNAs, and triggers of higher (HT) and lower (LT) EtOH-tolerant phenotypes. LncRNAs act in a strain-specific manner in the EtOH stress response. Network and omics analyses revealed that cells prepare for stress relief by favoring activation of life-essential systems. Therefore, longevity, peroxisomal, energy, lipid, and RNA/protein metabolisms are the core processes that drive EtOH tolerance. By integrating omics, network analysis, and several other experiments, we showed how the HT and LT phenotypes may arise: (1) the divergence occurs after cell signaling reaches the longevity and peroxisomal pathways, with CTA1 and ROS playing key roles; (2) signals reaching essential ribosomal and RNA pathways via SUI2 enhance the divergence; (3) specific lipid metabolism pathways also act on phenotype-specific profiles; (4) HTs take greater advantage of degradation and membraneless structures to cope with EtOH stress; and (5) our EtOH stress-buffering model suggests that diauxic shift drives EtOH buffering through an energy burst, mainly in HTs. Finally, critical genes, pathways, and the first models including lncRNAs to describe nuances of EtOH tolerance are reported here.
A Case of Mucopolysaccharidosis II Caused by a Novel Variant with Skin Linear Hyperpigmented Streaks along Blaschko’s Lines
Authors: Sofronova Viktoriia , Gurinova Elizaveta , Petukhova Diana , Fukamatsu Hiroko , Yamamoto Takenobu , Aoyama Yumi , Golikova Polina , Moskvitin Gavril , Ivanova Roza , Savvina Mira , Vasilev Filipp , Moriwaki Takahito , Terawaki Seigo , Sukhomyasova Aitalina , Maksimova Nadezhda , Otomo Takanobu
Published: March 2023

We report a case of an eight-year-old boy with mucopolysaccharidosis (MPS) II with atypical skin lesions of hyperpigmented streaks along Blaschko’s lines. This case presented with mild symptoms of MPS such as hepatosplenomegaly, joint stiffness, and quite mild bone deformity, which was the reason for the delay in diagnosis until the age of seven years. However, he showed an intellectual disability that did not meet the diagnostic criteria for an attenuated form of MPS II. Iduronate 2-sulfatase activity was reduced. Clinical exome sequencing of DNA from peripheral blood revealed a novel pathogenic missense variant (NM_000202.8(IDS_v001):c.703C>A, p.(Pro235Thr)) in the IDS gene, which was confirmed in the mother with a heterozygous state. His brownish skin lesions differed from the Mongolian blue spots or “pebbling” of the skin that are observed in MPS II.
An In Vitro Analysis of TKI-Based Sequence Therapy in Renal Cell Carcinoma Cell Lines
Authors: Zaccagnino Angela , Vynnytska-Myronovska Bozhena , Stöckle Michael , Junker Kerstin
Published: March 2023

The tyrosine kinase inhibitor (TKI) cabozantinib might impede the growth of the sunitinib-resistant cell lines by targeting MET and AXL overexpression in metastatic renal cell carcinoma (mRCC). We studied the role of MET and AXL in the response to cabozantinib, particularly following long-term administration with sunitinib. Two sunitinib-resistant cell lines, 786-O/S and Caki-2/S, and the matching 786-O/WT and Caki-2/WT cells were exposed to cabozantinib. The drug response was cell-line-specific. The 786-O/S cells were less growth-inhibited by cabozantinib than 786-O/WT cells (p-value = 0.02). In 786-O/S cells, the high level of phosphorylation of MET and AXL was not affected by cabozantinib. Despite cabozantinib hampering the high constitutive phosphorylation of MET, the Caki-2 cells showed low sensitivity to cabozantinib, and this was independent of sunitinib pretreatment. In both sunitinib-resistant cell lines, cabozantinib increased Src-FAK activation and impeded mTOR expression. The modulation of ERK and AKT was cell-line-specific, mirroring the heterogeneity among the patients. Overall, the MET- and AXL-driven status did not affect cell responsiveness to cabozantinib in the second-line treatment. The activation of Src-FAK might counteract cabozantinib activity and contribute to tumor survival and may be considered an early indicator of therapy response.
Cornus officinalis Seed Extract Inhibits AIM2-Inflammasome Activation and Attenuates Imiquimod-Induced Psoriasis-like Skin Inflammation
Authors: Lee Se-Bin , Kang Ju-Hui , Sim Eun-Jung , Jung Ye-Rin , Kim Jeong-Hyeon , Hillman Prima F. , Nam Sang-Jip , Kang Tae-Bong
Published: March 2023

The AIM2 inflammasome is an innate immune system component that defends against cytosolic bacteria and DNA viruses, but its aberrant activation can lead to the progression of various inflammatory diseases, including psoriasis. However, there have been few reports of specific inhibitors of AIM2 inflammasome activation. In this study, we aimed to investigate the inhibitory activity of ethanolic extracts of seeds of Cornus officinalis (CO), a herb and food plant used in traditional medicine, on AIM2-inflammasome activation. We found that CO inhibited the release of IL-1β induced by dsDNA in both BMDMs and HaCaT cells, but that it showed no effect on the release of IL-1β induced by NLRP3 inflammasome triggers, such as nigericin and silica, or the NLRC4 inflammasome trigger flagellin. Furthermore, we demonstrated that CO inhibited the cleavage of caspase-1, an inflammasome activation marker, and an upstream event, the translocation and speck formation of ASC. In addition, further experiments and mechanistic investigations revealed that CO can inhibit AIM2 speck formation induced by dsDNA in AIM2-overexpressing HEK293T cells. To verify the correlation in vivo, we investigated the efficacy of CO in an imiquimod (IMQ)-induced psoriasis model, which has reported associations with the AIM2 inflammasome. We found that topical application of CO alleviated psoriasis-like symptoms, such as erythema, scaling, and epidermal thickening, in a dose-dependent manner. Moreover, CO also significantly decreased IMQ-induced expression of AIM2 inflammasome components, including AIM2, ASC, and caspase-1, and led to the elevation of serum IL-17A. In conclusion, our results suggest that CO may be a valuable candidate for the discovery of AIM2 inhibitors and the regulation of AIM2-related diseases.
The Cell Transformation Assay: A Historical Assessment of Current Knowledge of Applications in an Integrated Approach to Testing and Assessment for Non-Genotoxic Carcinogens
Authors: Colacci Annamaria , Corvi Raffaella , Ohmori Kyomi , Paparella Martin , Serra Stefania , Da Rocha Carrico Iris , Vasseur Paule , Jacobs Miriam Naomi
Published: March 2023

The history of the development of the cell transformation assays (CTAs) is described, providing an overview of in vitro cell transformation from its origin to the new transcriptomic-based CTAs. Application of this knowledge is utilized to address how the different types of CTAs, variously addressing initiation and promotion, can be included on a mechanistic basis within the integrated approach to testing and assessment (IATA) for non-genotoxic carcinogens. Building upon assay assessments targeting the key events in the IATA, we identify how the different CTA models can appropriately fit, following preceding steps in the IATA. The preceding steps are the prescreening transcriptomic approaches, and assessment within the earlier key events of inflammation, immune disruption, mitotic signaling and cell injury. The CTA models address the later key events of (sustained) proliferation and change in morphology leading to tumor formation. The complementary key biomarkers with respect to the precursor key events and respective CTAs are mapped, providing a structured mechanistic approach to represent the complexity of the (non-genotoxic) carcinogenesis process, and specifically their capacity to identify non-genotoxic carcinogenic chemicals in a human relevant IATA.
Analogues of Anticancer Natural Products: Chiral Aspects
Authors: Valentová Jindra , Lintnerová Lucia , Miklášová Natalia , Oboňová Bianka , Habala Ladislav
Published: March 2023

Life is chiral, as its constituents consist, to a large degree, of optically active molecules, be they macromolecules (proteins, nucleic acids) or small biomolecules. Hence, these molecules interact disparately with different enantiomers of chiral compounds, creating a preference for a particular enantiomer. This chiral discrimination is of special importance in medicinal chemistry, since many pharmacologically active compounds are used as racemates—equimolar mixtures of two enantiomers. Each of these enantiomers may express different behaviour in terms of pharmacodynamics, pharmacokinetics, and toxicity. The application of only one enantiomer may improve the bioactivity of a drug, as well as reduce the incidence and intensity of adverse effects. This is of special significance regarding the structure of natural products since the great majority of these compounds contain one or several chiral centres. In the present survey, we discuss the impact of chirality on anticancer chemotherapy and highlight the recent developments in this area. Particular attention has been given to synthetic derivatives of drugs of natural origin, as naturally occurring compounds constitute a major pool of new pharmacological leads. Studies have been selected which report the differential activity of the enantiomers or the activities of a single enantiomer and the racemate.
A Survey on Compression Domain Image and Video Data Processing and Analysis Techniques
Authors: Dong Yuhang , Pan W. David
Published: March 2023

A tremendous amount of image and video data are being generated and shared in our daily lives. Image and video data are typically stored and transmitted in compressed form in order to reduce storage space and transmission time. The processing and analysis of compressed image and video data can greatly reduce input data size and eliminate the need for decompression and recompression, thereby achieving significant savings in memory and computation time. There exists a body of research on compression domain data processing and analysis. This survey focuses on the work related to image and video data. The papers cited are categorized based on their target applications, including image and video resizing and retrieval, information hiding and watermark embedding, image and video enhancement and segmentation, object and motion detection, as well as pattern classification, among several other applications. Key methods used for these applications are explained and discussed. Comparisons are drawn among similar approaches. We then point out possible directions of further research.
Critical Government Strategies for Enhancing Building Information Modeling Implementation in Indonesia
Authors: Rani Hafnidar A. , Al-Mohammad Mohammad S. , Rajabi Mohammad Sadra , Rahman Rahimi A.
Published: March 2023

Building information modeling (BIM) enables substantial improvement in the architect, engineering, and construction (AEC) industry. To enhance BIM implementation, policymakers should develop appropriate strategies addressing local AEC industry needs. However, prior works that have explored government strategies to enhance BIM implementation in Indonesia are scarce. Therefore, this study aimed to investigate the critical government strategies to enhance BIM implementation in Indonesia. A systematic literature review and semi-structured interviews with AEC professionals yielded 12 potential government strategies. The data were analyzed using mean score ranking, normalization, overlap, agreement, and correlation analyses. The findings illustrate that six strategies are critical for enhancing BIM implementation in Indonesia. Two of the six strategies overlapped between all main construction project stakeholders (i.e., consultants, contractors, and clients): (1) develop programs to integrate BIM into education curricula and academia, and (2) develop BIM implementation guidelines. These two strategies were highly correlated, and all project stakeholders had consistent views on their criticality for enhancing BIM implementation in Indonesia. The findings benefit policymakers by highlighting specific strategies that should take place to enhance BIM implementation in Indonesia.
Inorganic Sorbents for Wastewater Treatment from Radioactive Contaminants
Authors: Nekrasova Natalya A. , Milyutin Vitaly V. , Kaptakov Victor O. , Kozlitin Evgeny A.
Published: March 2023

The article presents the distribution coefficient (Kd) values of 137Cs and 90Sr tracer radionuclides in solutions of sodium and calcium salts for a wide range of commercially available inorganic sorbents: natural and synthetic aluminosilicates, manganese, titanium and zirconium oxyhydrates, titanium and zirconium phosphates, titanosilicates of alkali metals, and ferrocyanides of transition metals. The results were obtained using a standard technique developed by the authors for evaluating the efficiency of various sorption materials towards cesium and strontium radionuclides. It was shown that bentonite clays and natural and synthetic zeolites are the best for decontaminating low-salt natural water from cesium radionuclides, and ferrocyanide sorbents are the choice for decontaminating high-salt-bearing solutions. The manganese (III, IV) oxyhydrate-based MDM sorbent is the most effective for removing strontium from natural water; for seawater, the barium silicate-based SRM-Sr sorbent is the first-in-class. Results of the study provide a possibility of making a reasonable choice of sorbents for the most effective treatment of natural water and technogenic aqueous waste contaminated with cesium and strontium radionuclides.
Electrocardiographic and other Noninvasive Hemodynamic Markers in Decompensated CHF Patients
Authors: Piccirillo Gianfranco , Moscucci Federica , Mezzadri Martina , Caltabiano Cristina , Di Diego Ilaria , Carnovale Myriam , Corrao Andrea , Stefano Sara , Scinicariello Claudia , Giuffrè Marco , De Santis Valerio , Sciomer Susanna , Rossi Pietro , Magrì Damiano
Published: March 2023

Acutely decompensated chronic heart failure (adCHF) is among the most important causes of in-hospital mortality. R-wave peak time (RpT) or delayed intrinsicoid deflection was proposed as a risk marker of sudden cardiac death and heart failure decompensation. Authors want to verify if QR interval or RpT, obtained from 12-lead standard ECG and during 5-min ECG recordings (II lead), could be useful to identify adCHF. At hospital admission, patients underwent 5-min ECG recordings, obtaining mean and standard deviation (SD) of the following ECG intervals: QR, QRS, QT, JT, and T peak–T end (Te). The RpT from a standard ECG was calculated. Patients were grouped by the age-stratified Januzzi NT-proBNP cut-off. A total of 140 patients with suspected adCHF were enrolled: 87 (mean age 83 ± 10, M/F 38/49) with and 53 (mean age: 83 ± 9, M/F: 23/30) without adCHF. V5-, V6- (p < 0.05) RpT, and QRSD, QRSSD, QTSD, JTSD, and TeSDp < 0.001 were significantly higher in the adCHF group. Multivariable logistic regression analysis demonstrated that the mean of QT (p < 0.05) and Te (p < 0.05) were the most reliable markers of in-hospital mortality. V6 RpT was directly related to NT-proBNP (r: 0.26, p < 0.001) and inversely related to a left ventricular ejection fraction (r: 0.38, p < 0.001). The intrinsicoid deflection time (obtained from V5-6 and QRSD) could be used as a possible marker of adCHF.
BioMimics 3D Stent in Femoropopliteal Lesions: 3-Year Outcomes with Propensity Matching for Drug-Coated Balloons
Authors: Piorkowski Michael , Zeller Thomas , Rammos Christos , Deloose Koen , Hertting Klaus , Sesselmann Volker , Tepe Gunnar , Gaines Peter , Lichtenberg Michael
Published: March 2023

Background: Through its helical centreline geometry, the BioMimics 3D vascular stent system is designed for the mobile femoropopliteal region, aiming to improve long-term patency and the risk of stent fractures. Methods: MIMICS 3D is a prospective, European, multi-centre, observational registry to evaluate the BioMimics 3D stent in a real-world population through 3 years. A propensity-matched comparison was performed to investigate the effect of the additional use of drug-coated balloons (DCB). Results: The MIMICS 3D registry enrolled 507 patients (518 lesion, length 125.9 ± 91.0 mm). At 3 years, the overall survival was 85.2%, freedom from major amputation 98.5%, freedom from clinically driven target lesion revascularisation 78.0%, and primary patency 70.2%. The propensity-matched cohort included 195 patients in each cohort. At 3-year follow-up, there was no statistically significant difference in clinical outcomes, such as overall survival (87.9% in the DCB vs. 85.1% in the no DCB group), freedom from major amputation (99.4% vs. 97.2%), clinically driven TLR (76.4% vs. 80.3%), and primary patency (68.5% vs. 74.4%). Conclusion: The MIMICS 3D registry showed good 3-year outcomes of the BioMimics 3D stent in femoropopliteal lesions, demonstrating the safety and performance of this device under real-world conditions, whether used alone or in combination with a DCB.
Association between Preoperative 18-FDG PET-CT SUVmax and Next-Generation Sequencing Results in Postoperative Ovarian Malignant Tissue in Patients with Advanced Ovarian Cancer
Authors: Ryu Jung Min , Jeong Yoon Young , Lee Sun-Jae , Choi Byung Wook , Choi Youn Seok
Published: March 2023

This study investigated the association between maximum standardized uptake values (SUVmax) on preoperative 18-FDG PET-CT and next-generation sequencing (NGS) results in post-surgical ovarian malignant tissue in patients with advanced ovarian cancer. Twenty-five patients with stage IIIC or IV ovarian cancer who underwent both preoperative 18-FDG PET-CT and postoperative NGS for ovarian malignancies were retrospectively enrolled. Two patients had no detected variants, 21 of the 23 patients with any somatic variant had at least one single nucleotide variant (SNV) or insertion/deletion (indel), 10 patients showed copy number variation (CNV), and two patients had a fusion variant. SUVmax differed according to the presence of SNVs/indels, with an SUVmax of 13.06 for patients with ≥ 1 SNV/indel and 6.28 for patients without (p = 0.003). Seventeen of 20 patients with Tier 2 variants had TP53 variants, and there was a statistically significant association between SUVmax and the presence of TP53 variants (13.21 vs. 9.35, p = 0.041). Analysis of the correlation between the sum of the Tier 1 and Tier 2 numbers and SUVmax showed a statistically significant correlation (p = 0.002; Pearson’s r = 0.588). In conclusion, patients with advanced ovarian cancer with SNVs/indels on NGS, especially those with TP53 Tier 2 variants, showed a proportional association with tumor SUVmax on preoperative PET-CT.
Isolated Subclinical Right Ventricle Systolic Dysfunction in Patients after Liver Transplantation
Authors: Celiker Guler Emel , Omaygenc Mehmet Onur , Naki Deniz Dilan , Yazar Arzu , Karaca Ibrahim Oguz , Korkut Esin
Published: March 2023

Although hemodynamic alterations in end-stage liver disease (ESLD) and its association with porto-pulmonary hypertension have been well-established, the long-term effects of ESLD on RV systolic function in patients without porto-pulmonary hypertension remain disregarded. Here we aimed to assess the long-term effect of ESLD on RV function and its relationship with the use of NSBBs and clinical, laboratory and imaging parameters in end-stage liver disease. The use of NSBBs is still controversial due to concerns about reduced cardiac contractility and the possibility of increased mortality. Thirty-four liver transplant recipients were included. Demographic characteristics, laboratory and baseline echocardiography measures were obtained. Patients were recalled for transthoracic echocardiographic evaluation after transplantation. Right ventricle dysfunction was identified by having at least one value below the reference levels of RV S’, or TAPSE. Isolated subclinical RV dysfunction was observed at 20.6% of the sample population. The present study demonstrates hemodynamic circulation in cirrhosis and increased preload and afterload might have long-term effects on RV function, even the lack of porto-pulmonary hypertension. These findings underline the significance of cardiac function follow-up in cirrhotic patients after transplantation. In this study, patients treated with propranolol seemed to have better RV function and less gastrointestinal bleeding. We speculated that preoperative propranolol treatment might help preserve RV function by providing RAS suppression, improving endothelial function and hyperdynamic circulation seen in ESLD. This potential protective relationship between the use of propranolol and RV function might improve mortality or graft-failure during OLT and after liver transplantation in patients with cirrhosis.
The Photocoagulation-Assisted Aesthetic Treatment of Phlebectasias of the Lips Using a Nd:YAG Laser with a Low Energy Level
Authors: Armogida Niccolò Giuseppe , Valletta Alessandra , Calabria Elena , Canfora Federica , Rengo Carlo , Spagnuolo Gianrico
Published: March 2023

Venous lakes (VLs) are benign malformations often localized in the lips, therefore impacting the self-confidence of patients. In the present study, the use of a Nd:YAG laser according to a defined protocol with a low level of absolute energy (4.9 J) is proposed for the treatment of VLs of the lips. A total of 47 patients with 50 labial VL were treated with a Nd:YAG in one laser session without local anesthesia. The area reduction was evaluated according to the Vlachakis criteria 7 and 30 days after the laser application. Additionally, oral discomfort was rated according to the Numeric Rating Scale (NRS) during and 24 hours after the procedure. All patients achieved complete clinical healing within 30 days after the laser application. In particular, patients with VL with a diameter ≤ 6 mm (62.1%) achieved a complete resolution after 7 days (p-value < 0.001). Such patients reported little or no discomfort (NRS 0 to 3) during the laser session and no discomfort after 24 hours (p-value < 0.001). No major complications were reported, and no recurrence was observed at 2-year follow-up. These findings suggest that Nd:YAG laser treatment could be an effective and well-tolerated approach to the aesthetic treatment of labial VL.
Transcranial Doppler Echography Measurement in Hemodialysis Patients: The Potential Role of Angiotensin II Receptor Blockades on Cerebrovascular Circulation
Authors: Maesato Kyoko , Kobayashi Shuzo , Ohtake Takayasu , Mochida Yasuhiro , Ishioka Kunihiro , Oka Machiko , Moriya Hidekazu , Hidaka Sumi
Published: March 2023

Background: Although hemodialysis (HD) patients have an elevated risk of strokes, there are few reports about transcranial doppler (TCD) echography measurements. It is well-known that angiotensin II receptor blockades (ARBs) protect against cardiovascular complications. In this study, we measured intracranial artery (ICA) velocity using TCD echography and studied the associated factors with its velocity in HD patients by a comparison with or without ARBs. Methods: We conducted a cross-sectional study in a single hospital. We included 61 patients who had measurable ICA velocity by TCD echography. Among them, the ARB usage group consisted of 22 subjects, whilst the non-ARB usage group consisted of 39 subjects. Results: Patients in the ARB (+) and ARB (-) groups did not show any difference in basic characteristics. ICA blood flow velocity in all intracranial arteries tended to show greater values in the ARB group than those in the non-ARB group. Particularly, blood velocity in the middle cerebral artery (MCA) (maximal flow velocity) statistically increased in the ARB group, respectively. In a univariate analysis, MCA maximum velocity was significantly associated with ARB usage (p = 0.011) and low hematocrit levels (p = 0.045). The multivariate analysis chose only ARB usage as an independent factor associated with left MCA maximum velocity (p = 0.022). Conclusions: We showed that dialysis patients with ARBs have significantly higher ICA blood velocity. ARBs might have a potential benefit for maintaining ICA blood flow in HD patients.
“In Situ” Methotrexate Injection Followed by Hysteroscopic Resection for Caesarean Scar Pregnancy: A Single-Center Experience
Authors: El Sabbagh Anthony , Sayour Ihsan , Sleiman Zaki , Centini Gabriele , Lazzeri Lucia , Giorgi Matteo , Zupi Errico , Habib Nassir
Published: March 2023

Background: We evaluated the efficacy of local methotrexate (MTX) treatment followed by hysteroscopic resection for caesarean scar pregnancy and its impact on future fertility. Methods: Monocentric, prospective, observational study performed in the Haykel Hospital between June 2016 and December 2020. Twenty-one women with caesarean scar pregnancy underwent a transcutaneous ultrasound-guided direct injection of MTX into the gestational sac in an outpatient setting. Hysteroscopic resection of residual trophoblastic retention was then performed according to perisaccular blood flow. Main results: Two patients had complete spontaneous trophoblast expulsion after MTX injection, and hysteroscopy was performed in 19 patients for residual trophoblastic retention 1 to 12 weeks after MTX injection. Successful preservation of a healthy uterus with the combined procedure was obtained in 94.8% of patients. Hemostatic hysterectomy was required in one patient. Mean hospitalization duration was 1.5 days. Three patients had spontaneous pregnancy after the procedure. Conclusion: Direct MTX injection into the gestational sac for caesarean scar pregnancy followed by hysteroscopic resection was an effective technique with a short hospitalization, fertility preservation and a low major complication rate compared with other modalities of treatment reported in the literature. Further larger prospective comparative studies are needed to confirm the efficacy of this procedure.
Association of Chronic Heart Failure with Frailty, Malnutrition, and Sarcopenia Parameters in Older Patients—A Cross-Sectional Study in a Geriatric Ward
Authors: Charkiewicz Miroslaw , Wojszel Zyta Beata , Kasiukiewicz Agnieszka , Magnuszewski Lukasz , Wojszel Aleksandra
Published: March 2023

The need to assess sarcopenia and frailty in patients with chronic heart failure (HF) has recently been raised. This cross-sectional study of 416 geriatric ward patients (median age (Me)—82 (IQR, 77–86) years, 77.4% female, 96.9% community dwelling) aimed to assess the prevalence of dynapenia, frailty syndrome, functional and nutritional health, and co-morbidity regarding their HF status. We collected data from comprehensive geriatric assessment. We observed HF in 162 (38.9%) patients, with 80 (49.4%) classified as New York Heart Association (NYHA) class III or IV. HF patients were significantly older, more frequently male, obese, hospitalized in the previous year, burdened with multimorbidity and polypharmacy, classified as frail, dependent on daily living activities, and physically non-active. Ischemic heart disease, atrial fibrillation, diabetes, peripheral arterial disease, anemia, chronic kidney disease, history of myocardial infarction, and stroke were found significantly more often in the HF group. A considerably higher percentage of HF patients had dynapenia (54.9% versus 41.9%, p = 0.02), but the difference was significant only in women. We found no significant difference between HF and no-HF groups regarding muscle performance, except for lower median gait speed in the HF group—0.53 m/s (0.35–0.89 m/s) versus 0.68 m/s (0.44–0.99 m/s), p = 0.02). HF patients significantly more often had low grip strength accompanied by slow gait, suggesting probable severe sarcopenia (40.4% vs. 29% in patients without HF, p = 0.046). In the regression analysis, significantly higher odds for HF were observed for lower mid-arm circumference (MAC) and dynapenia when controlling for age, sex, body mass index (BMI), calf circumference (CC), peripheral arterial disease, history of stroke, ischemic heart disease, atrial fibrillation, and diabetes mellitus. Conclusions: HF geriatric patients are often burdened with frailty, obesity, multimorbidity, and polypharmacy. As a result, they are more likely to present low muscle strength (potential sarcopenia), which is frequently accompanied by functional limitations (suggestive of more advanced stages of sarcopenia). This tendency is evident mainly in older women. Nevertheless, sarcopenia can be independently associated with HF in older patients with multimorbidity and disability who are hospitalized in a geriatric department, as a multivariable logistic regression analysis demonstrated.
Proof-of-Concept Double-Blind Placebo-Controlled Trial Measuring Cartilage Composition in Early Rheumatoid Arthritis under TNF-α-Inhibitor Therapy
Authors: Frenken Miriam , Ostendorf Benedikt , Brinks Ralph , Schleich Christoph , Wilms Lena M. , Vordenbäumen Stefan , Müller-Lutz Anja , Richter Jutta G. , Sander Oliver , Antoch Gerald , Schneider Matthias , Baraliakos Xenofon , Abrar Daniel B. , Sewerin Philipp
Published: March 2023

Low levels of delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) values are indicative of cartilage degeneration. Patients with early rheumatoid arthritis are known to have low dGEMRIC values due to inflammatory activity. The additional effect of biological disease-modifying antirheumatic drug (bDMARD) and conventional synthetic disease-modifying antirheumatic drug (csDMARD) treatment on cartilage status is still unclear. In this prospective, double-blinded, randomized proof-of-concept clinical trial, patients with early rheumatoid arthritis (disease duration less than 12 months from symptoms onset) were treated with methotrexate + adalimumab (10 patients: 6/4 (f/m)). A control group with methotrexate alone (four patients: 2/2 (f/m)) was used. Cartilage integrity in the metacarpophalangeal joints was compared using dGEMRIC at baseline, 12, and 24 weeks after treatment initiation. A statistically significant increase in dGEMRIC levels was found in the adalimumab group considering the results after 12 and 24 weeks of therapy (p < 0.05) but not in the control group (p: non-significant). After 24 weeks, a tendency towards increased dGEMRIC values under combination therapy was observed, whereas methotrexate alone showed a slight decrease without meeting the criteria of significance (dGEMRIC mean change: +85.8 ms [−156.2–+346.5 ms] vs. 30.75 ms [−273.0–+131.0 ms]; p: non-significant). After 24 weeks of treatment with a combination of methotrexate and adalimumab, a trend indicating improvement in cartilage composition is seen in patients with early rheumatoid arthritis. However, treatment with methotrexate alone showed no change in cartilage composition, as observed in dGEMRIC sequences of metacarpophalangeal joints.
Participant Recruitment Issues in Child and Adolescent Psychiatry Clinical Trials with a Focus on Prevention Programs: A Meta-Analytic Review of the Literature
Authors: Kilicel Deniz , De Crescenzo Franco , Pontrelli Giuseppe , Armando Marco
Published: March 2023

Introduction: There is a strong need to conduct rigorous and robust trials for children and adolescents in mental health settings. One of the main barriers to meeting this requirement is the poor recruitment rate. Effective recruitment strategies are crucial for the success of a clinical trial, and therefore, we reviewed recruitment strategies in clinical trials on children and adolescents in mental health with a focus on prevention programs. Methods: We reviewed the literature by searching PubMed/Medline, the Cochrane Library database, and Web of Science through December 2022 as well as the reference lists of relevant articles. We included only studies describing recruitment strategies for pediatric clinical trials in mental health settings and extracted data on recruitment and completion rates. Results: The search yielded 13 studies that enrolled a total of 14,452 participants. Overall, studies mainly used social networks or clinical settings to recruit participants. Half of the studies used only one recruitment method. Using multiple recruitment methods (56.6%, 95%CI: 24.5–86.0) resulted in higher recruitment. The use of monetary incentives (47.0%, 95%CI: 24.6–70.0) enhanced the recruitment rate but not significantly (32.6%, 95%CI: 15.7–52.1). All types of recruitment methods showed high completion rates (82.9%, 95%CI: 61.7–97.5) even though prevention programs showed the smallest recruitment rate (76.1%, 95%CI: 50.9–94.4). Conclusions: Pediatric mental health clinical trials face many difficulties in recruitment. We found that these trials could benefit from faster and more efficient recruitment of participants when more than one method is implemented. Social networks can be helpful where ethically possible. We hope the description of these strategies will help foster innovation in recruitment for pediatric studies in mental health.
Serum Level of Protein-Bound Uraemic Toxins in Haemodialysis Patients with Chronic Kidney Disease-Associated Pruritus: Myths and Facts
Authors: Świerczyńska-Mróz Karolina , Nowicka-Suszko Danuta , Fleszar Mariusz G. , Fortuna Paulina , Krajewski Piotr K. , Krajewska Magdalena , Białynicki-Birula Rafał , Szepietowski Jacek C.
Published: March 2023

Recent studies place great importance on Protein-Bound Uraemic Toxins (PBUT) in the context of etiopathogenesis of chronic kidney disease-associated pruritus (CKD-aP). This study aimed to investigate the possible contribution of free and total Indoxyl Sulfate (IS) and p-Cresol Sulfate (PCS) to the cause of CKD-aP. Group A included 64 patients on maintenance haemodialysis (HD) with CKD-aP. Group B included 62 patients on maintenance HD that did not report CKD-aP, and group C included 50 healthy controls. Pruritus severity was assessed using a Numerical Rating Scale (NRS). Moreover, other tools like UP-Dial, ItchyQoL, and the 4-Item Itch Questionnaire evaluating CKD-aP were completed by the patients. The serum levels of free and total IS and PCS concentrations were measured using the Ultra Performance Liquid Chromatography System. No significant difference in the serum level of free and total IS, or PCS, was observed between the patients who reported CKD-aP and those without pruritus. Moreover, there was no correlation between serum IS or PCS levels and the severity of the itch. Our study does not support earlier findings about higher levels of IS and PCS in patients reporting CKD-aP. Further studies will be needed to investigate these discrepancies as well as to understand the cause of CKD-aP.
The Effectiveness and Tolerability of Atropine Eye Drops for Myopia Control in Non-Asian Regions
Authors: Simonaviciute Dovile , Grzybowski Andrzej , Lanca Carla , Pang Chi Pui , Gelzinis Arvydas , Zemaitiene Reda
Published: March 2023

Myopia is the most common ocular disorder worldwide with an increasing prevalence over the past few decades. It is a refractive error associated with excessive growth of the eyeball. Individuals with myopia, especially high myopia, are prone to develop sight-threatening complications. Currently, atropine is the only drug that is used to slow myopia progression in clinical practice. However, there are still areas of uncertainty such as treatment strategy, optimal concentration when considering risk–benefit ratio and active treatment period. Since the prevalence of myopia is much higher in Asian countries, most of the research on myopia control has been conducted in Asia. Data on the efficacy and tolerability to atropine eye drops in the non-Asian population remains limited. In this review, we summarize the results of published clinical trials on the effectiveness and tolerability of atropine eye drops for myopia control in non-Asian regions. The efficacy was evaluated by the mean change in spherical equivalent (SE) or axial length (AL). The tolerability of atropine eye drops was analyzed based on patients complains and adverse events. The results of this review suggest that 0.01% atropine eye drops are effective in non-Asian regions achieving less side effects compared to 0.5% concentration.
Man-Made Raw Materials for the Production of Composite Silicate Materials Using Energy-Saving Technology
Authors: Auyesbek Sultan , Sarsenbayev Nuraly , Abduova Aisulu , Sarsenbayev Bakhytzhan , Uderbayev Saken , Aimenov Zhambyl , Kenzhaliyeva Gulmira , Akishev Uzakbai , Aubakirova Taslima , Sauganova Gaukhar , Amanov Eldar , Kolesnikova Olga , Panarin Igor
Published: March 2023

This paper presents the development of composite silicate mass compositions based on man-made waste for the production of autoclave hardening products, as well as the results of physico-chemical studies of hydration products of silicate materials. The possibility, expediency and efficiency of using multi-tonnage technogenic waste of Kazakhstan in the industry of composite building materials is shown. Based on the results of the conducted research, the composition of a composite silicate mass based on burnt carbonate-barium tailings (8–12%), electrothermophosphoric slags (82–90%) or sand and dust from cement kiln electrofilters (2–5%) for the production of autoclave hardening products was developed. It was found that the cementing substance in composite silicate materials is represented by CSH(B) calcium silicate hydrates, tobermorite and serpentine. The simultaneous presence of fibrous and crystalline calcium and magnesium silicate hydrates in hydration products leads to the creation of composite products with a maximum strength of 41–49 MPa.