bluuebunny/crossref_metadata_2025 huggingface dataset:
The improved cycling stability of nanostructured NiCo2O4 anodes for lithium and sodium ion batteries
Authors: Tang Xinyue , Ren Qingqing , Yu Fu-Da , Wang Zhen-Bo
Published: April 2023

Developing the high-capacity anode materials such as conversion-type metal oxides which possess both Li and Na storage activity is very practical for the high-energy LIBs/SIBs. Herein, we use NiCo2O4anodes as a model to investigate the morphology evolution which accounts for the poor cycling performance and understand the effect of structure optimization on the electrochemical performance. Three NiCo2O4samples with different morphologies of microspheres, nanospheres and nanosheets are synthesized. Firstly, the serious structural degradation of NiCo2O4microspheres is observed whether it works as a LIB or SIB anode. In addition, a significant difference between the lithiation and sodiation capacity of NiCo2O4materials reveals Na+ions only partially intercalated in NiCo2O4and the conversion reaction limited by the strain. Next, NiCo2O4nanosheets on Ni foam as a binder-free anode for LIBs are investigated which suggest the positive effect of 3D nanostructures on the morphology stability. As a result, NiCo2O4nanosheets deliver a high lithiation capacity of 1092 mAh g− 1after 100 cycles at 0.5 A g− 1and an excellent rate capacity of 643 mAh g− 1at 4 A g− 1. Finally, NiCo2O4nanospheres are evaluted as a SIB anode which indicate the smaller particle size of active materials is beneficial to the release of stress and structure stability during discharge-charge processes. A rational design of the electrode’ architecture is very important for the conversion-type 3d transition metal oxide anodes for advanced LIBs and SIBs.
Distribution and prevalence of ixodid tick species (Acari: Ixodidae) infesting cattle from North-eastern Uganda (Karamoja)
Authors: Etiang Patrick , Musoba Abubakar , Nalumenya David , Ndekezi Christian , Bbira Johnson , Ochwo Sylvester , Tweyongyere Robert , Muhanguzi Dennis
Published: April 2023

Background Ticks and tick-borne diseases (TTBDs) are a significant threat to livestock production in sub-Saharan Africa. Transhumance pastoralism practiced in Karamoja region and other factors like cattle trade, communal grazing and the presence of wildlife predispose cattle to TTBDs. Tick species abundance and distribution data can be used as a tool for early disease diagnosis and inform tick control strategies. However, these data for north-eastern Uganda are currently limited; previous surveys were relatively localized and targeted fewer cattle kraals and numbers.Methods We randomly collected tick specimens from 1,534 cattle spread across Karamoja region in both the peak month of the rainy (May) and the dry (February) seasons. The ticks were identified using morpho-taxonomic keys and the tick species identities confirmed by the 16S rRNA gene sequencing and phylogenetic analysis.Results A collection of 18,637 ticks was examined and 15 tick species from three genera (10 Rhipicephalus; 3 Amblyomma; 2 Hyaloma species) were identified. Rhipicephalus appendiculatus was the most dominant (37.9%) tick specie, followed by Amblyomma variegatum (32.3%); A. lepidum (17.3%); R. evertsi evertsi (7.8%); and R. decoloratus (1.4%). Eight of these tick species were ubiquitous in the study districts while six were found in isolated areas. The peak month of the dry season collection was associated with a higher proportion of tick-infested cattle (91%) compared to the peak month of the rainy season (89.8%); a difference that we did not find statistically significant (χ2 = 0.5077, n = 1385, p = 0.476). The overall cattle infestation rate was mainly dominated by 5 tick species namely: A. variegatum (55%), R. appendiculatus (53%), A. lepidum (41%), R. Evertsi (22%), and R. Decoloratus (8%). Napak and Amudat districts registered the highest (95.4% and 94.9%) and lowest (80.9% and 80.7%) cattle tick infestation rates during the peak months of the rainy and dry seasons respectively. Rhipicephalus microplus was confirmed in Amudat, Kaabong and Napak districts.Conclusion This study demonstrates high cattle tick infestation rates by a battery of tick species in Karamoja region. We identified both R. microplus and R. decoloratus which indicates that R. microplus has recently been introduced in this region. This calls for effective tick control responses to prevent further spread of this invasive cattle tick specie.
Civil-Military Collaborative Governance in Disaster Management as a Defense Diplomacy Strategy for the Government of Indonesia (Case Study of the Aceh Earthquake and Tsunami in 2004)
Authors: Manurung Yanto , Maarif Syamsul , Toruan Tahan Samuel Lumban , Swastanto Yoedhi
Published: April 2023

This study aims to provide an understanding of the role of Civil-Military Collaborative Governance in disaster management in Aceh in 2004. This study used a qualitative research method with a case study approach. Data analysis in this study used descriptive-deductive techniques, besides that, the type of data used in this study was secondary data. Thus, the researcher conducted a search of the data contained in organizational reports and scientific articles. The results of this study show that Civil-Military Collaborative Governance in disaster management in Aceh in 2004 played a role as a defense diplomacy strategy for the Government of Indonesia.
Determination of optimal dietary valine concentrations for improved growth performance and innate immunity of juvenile Pacific white shrimp Litopenaeus vannamei
Authors: Ko Daehyun , Lee Chorong , Lee Kyeong-Jun
Published: April 2023

A study was conducted to evaluate dietary valine (Val) requirement for Pacific white shrimp (Litopenaeus vannamei). Five isonitrogenous (353 g/kg) and isocaloric (4.08 kcal/g) semi-purified diets containing graded levels of Val (3, 6, 9, 12 or 15 g/kg) were formulated. Quadruplicate groups of 12 shrimp (average body weight: 0.46 ± 0.00 g) were fed one of the experimental diets (2–5% of total body weight) for 11 weeks. Maximum weight gain was observed in 9 g/kg Val group. However, the growth performance was reduced when Val concentration in diets were higher than 12 g/kg. Shrimp fed the diets containing 3 g/kg Val showed significantly lower protein efficiency ratio, whole-body crude protein and Val concentrations. Dietary inclusion of Val significantly improved the relative expression of insulin-like growth factor binding protein and immune-related genes (prophenoloxidase, lysozyme and crustin) in the hepatopancreas and 9 g/kg Val group showed highest expression among all the groups. Polynomial regression analysis revealed that diet for juvenile L. vannamei requires to contain approximately 9.5 g/kg of Val (27 g/kg based on protein level) to ensure optimal growth and improving overall health.
Awareness, Knowledge and Practice Patterns of Oral Health and Hygiene among Speech-Language Pathologists in India: An Online Survey
Authors: Vijayan Syam Krishna , Bhattarai Biraj , N Swapna
Published: April 2023

Objectives The study aims to determine the level of awareness, knowledge, and practice patterns of Oral Health and Hygiene (OHH) among Speech-Language Pathologists (SLPs) in India.Materials & Method A questionnaire was developed to assess the awareness and knowledge regarding OHH among Master's students and professionals practicing speech-language pathology. The sections in the questionnaire included awareness and knowledge of oral health, knowledge of impact of poor oral health, and practices pertaining to assessment and management of oral health. The questionnaire was circulated among 200 SLPs practicing in India.Results A total of 162 participants responded to the survey. 93.2% participants were aware of the importance of OHH, however 42.6% participants did not feel confident enough to assess the same. Most (82.7%) agreed that poor OHH could lead to different health complications. 38.8% of the participants responded that they assessed oral health only sometimes and 54.3% reported that they never used standard screening/assessment tools to assess oral health. Only a very small percentage of participants (28.65%) counselled the patients for maintenance of oral hygiene and more than 40% never recommended appropriate OHH practices during management.Conclusion The present study is the first of its kind to assess the awareness, knowledge, and practice patterns of OHH among Indian SLPs. The results revealed that though the awareness and knowledge of OHH among the Indian SLPs was good, this did not reflect in their clinical practiceClinical Relevance: Collaborative efforts from professional and regulatory bodies and educational institutes are necessary in developing and implementing OHH-related aspects in India. This could potentially improve SLP's knowledge and clinical practices related to oral health and thereby improve patient outcomes.
The effect of hydroxyurea response on gut microbiota of beta-thalassemia major patients
Authors: Khan Ayesha , Khan Saeed , Ansari Saqib H. , Ahmed Ayaz , Iqbal Asif , Omair Muniza , Zeehan Nida , Isa Saman
Published: April 2023

Background Hydroxyurea (HU), a fetal hemoglobin inducer is effective in alleviating the symptoms of beta-thalassemia patients; however, its efficacy is not the same in all patients. Hydroxyurea metabolism in the gut might be responsible, which, as several studies suggest, impacts the metabolism of drugs and diseases including sickle cell diseasea and thalassemia. Hydroxyurea also influences the composition of the gut microbiota as evidenced by literature. In this study, we attempted to find a relationship between the gut microbiota and the response difference of hydroxyurea in beta-thalassemia major patients.Methods A total of 45 Beta-thalasssemia patients stool samples were collected. They were divided into 3 groups; responders, non-responders, and non-users of hydroxyurea. The samples of each group were pooled into 6 samples after DNA extraction. The V3-V4 hyper-variable region of the 16SrRNA gene was sequenced.Results The three groups did not differ in alpha diversity. Our study revealed significant differences in the gut microbiota of hydroxyurea responders, non-responders and non-users at genus and species level. Hydroxyurea response was found to be associated with butyrate producers belonging to phlyum Firmicutes. The responders were enriched in genera belonging to phylum Firmicutes especially Butyrate-producing bacteria such as Faecalibacterium, Butyrivibrio, Oscillobacter, Gemmiger, and Eubacterium. The non-responders were abundant in Prevotella, Mitsuokella, and Treponema. The non-users were abundant in Succinivibrio, followed by Bacteroides, and Megasphaera.Conclusion Our results suggest that the altered gut microbiota might be the reason for inter-individual variation of hydroxyurea and reveals various microbes that may serve as potential biomarkers for thalassemia or hydroxyurea efficacy.
Analysis and prediction of land use change characteristics in Nanchang from 2005 to 2035
Authors: Fu Shuai , Wang Hongbo , Zhong Rui , Xu Bingxian , Peng Yuxin
Published: April 2023

With the Rapid urban expansion and development, the contradiction between human and land has gradually emerged, and how to scientifically and rationally use land is an inescapable problem. Nanchang was selected to study in the study, land use change images of Landsat from 2005 to 2020 was analyzed, and predicted land use situation of Nanchang in 2035 by CA-Markov model, to analyze and evaluate the scientific and effective use of land resources in Nanchang. The results show that from 2005 to 2020, woodland continued to decrease, with a total decrease of 300.27 km2 in 15 years; unused land was developed into construction land and agricultural land, the proportion of unused land became less and less; the proportion of grassland and construction land increased, and the expansion rate of construction land increased. Agricultural land, woodland, grassland and water area are greatly affected by elevation slope; The construction land is greatly affected by the convenience of transportation, and the old construction land as the initial boundary of expansion has driven the transformation of surrounding land use. In 2035, agricultural land will remain largely stable; wood land will slightly decrease; both grassland and water area show a decreasing trend; the construction land increase by 306.31km2. Understanding the characteristics and laws of land use change in Nanchang is conducive to urban sustainable development planning. Simultaneous development and protection are conducive to the sustainable use of land resources and sustainable regional development.
Preliminary exploration of the effects of environmental factors on the microsatellite status of BRAF mutated colorectal cancer
Authors: Tian Binle , Chen Guiming , Shi Xiaoqin , Jiang Liren , Jiang Tao , Li Qi , Yuan Lin , Qin Jian
Published: April 2023

Background
To investigate the expression of EBV products and incidence of Gallstone disease (GD) among different microsatellite status in colorectal cancer (CRC) with BRAFV600E mutation.
Methods
We collected 30 CRC patients with BRAFV600E mutation and 10 BRAF (-) CRC patients as well as 54 healthy subjects. Tumor tissue samples were collected to detect the mutation of BRAF, KRAS, and TP53. Microsatellite status was determined by immunohistochemistry and PCR. EBER in situ hybridization was performed to detect EBV. In addition, we also collected clinical information about the patients.
Results
We found that although EBV products were detected in CRC, there were no significant differences in the EBV distribution between the different BRAF groups. Our study demonstrated that BRAFV600E mutation and BRAFV600E with MSI were significantly more frequent in the right CRC. Furthermore, the KRAS mutation rate in the BRAF-wild type group was proved to be significantly higher than that in the BRAF mutation group. In addition, we revealed that BRAF mutation and MSI were independent risk factors of TNM stage. The incidence of GD was higher in CRC patients than in general population, and although there was no significant difference between CRC with or without BRAFV600E mutation, the highest incidence of GD was found in MSS CRC with BRAFV600E mutation.
Conclusions
EBV plays a role in CRC, but is not a determinant of different microsatellite status in CRC with BRAFV600E mutation. The incidence of GD in MSS CRC with BRAFV600E mutation is significantly higher than that in the general population.
Association joint exposure to zinc, arsenic, cadmium, and lead with blood pressure
Authors: Wu Jingjing , Huang Miao , Huang Zhijun , Yuan Hong
Published: April 2023

Background
Previous studies have demonstrated the adverse effect of single metal on blood pressure (BP) by oxidative stress, however, few studies investigated the joint effect of multi-metals co-exposure on BP. We aimed to estimate the association between multi-metal co-exposure and BP.
Methods
2,764 residents living in heavy metal-contaminated areas of China for at least 5 years were recruited from August 2016 to July 2017 in this study. The average concentration of plasma zinc (Zn) and lead (Pb) and urine arsenic (As) and cadmium (Cd) were measured using an inductively coupled plasma mass spectrometer. The Ordinary least squares (OLS) linear regression and Bayesian kernel machine regression (BKMR) were utilized to assess the effect of the metals on systolic blood pressure (SBP) and diastolic blood pressure (DBP). All the metals were naturally log-transformed and As and Cd in urine were adjusted for urine creatinine levels.
Results
A total of 1,859 participants (58.40 ± 13.42 years old, 40.02% men) were included in the final analysis. The OLSlinear analyses showed that Zn and As were positively associated with SBP and DBP, while Cd was negatively associated with SBP and DBP. In the BKMR, we found a significantly positive joint effect of the metal mixture on BP when all the metal concentrations were above their medians. We also found the effects of the interaction between As and Zn on BP.
Conclusions
In conclusion, our study showed that multi-metal co-exposures caused an increase in BP in the population. Further prospective studies with larger sample sizes are necessary to validate this finding.
Effect of adhesives and attachment substrates on Sargassaceae zygote attachment
Authors: Miao Hang , Yang Qifan , Wang Huijie , Zhu Weidong , Bi Yuanxin
Published: April 2023

To address the problem of survival of macroalgal zygote transplants, six adhesives were mixed with Sargassaceae zygotes and incubated to determine their adhesion and germination. The adhesives with the highest zygote survival were selected, and then 4% alginate and 3% CaCl2 were mixed with the zygotes and applied to three adhesion substrates with different levels of roughness. The zygote attachment density was used to determine the combination of adhesives and substrates that was most favorable for zygote adhesion. The results showed that: (1) The zygote attachment density of coated alginate reached 259 ind. cm− 2, with a survival rate of 9.4. Although the attachment density of zygotes encapsulated with hyperbranched polymeric adhesive (HBPA) was low, the material was viscous, adhesive, and slow to dissolve, giving it potential as an outdoor transplant adhesion material. (2) The attachment density of zygotes on brick, cement board, and PVC substrates sprayed with 3% CaCl2 reached 86 ind. cm− 2, 48 ind. cm− 2, and 8 ind. cm− 2, and the survival rate was 52%, 29%, and 5%, respectively. The rougher the substrate, the more zygotes adhered. Therefore, in macroalgae restoration and seaweed farm reconstruction, artificial algal reefs with a rough substrate and granular dents could be used in combination with the application of adhesives. The results of this study will help to address the environmental constraints affecting the artificial transplantation of macroalgae and aid in the development of innovative habitat restoration techniques for macroalgae habitats.
Episode I. Exploring Streptococcus pneumoniae capsular typing through MALDI-TOF mass spectrometry and machine-learning algorithms in Argentina: Identifying prevalent NON PCV13 serotypes alongside PCV13 serotypes.
Authors: Zintgraff Jonathan , Zintgraff Jonathan , Rocca Florencia , Rocca Florencia , Eluchans Nahuel Sánchez , Eluchans Nahuel Sánchez , Irazu Lucía , Irazu Lucía , Moscoloni Maria Alicia , Moscoloni Maria Alicia , Lara Claudia , Lara Claudia , Santos Mauricio , Santos Mauricio
Published: April 2023

Laboratory surveillance of Streptococcus pneumoniae serotypes is crucial for the successful implementation of vaccines to prevent invasive pneumococcal diseases. The reference method of serotyping is the Quellung reaction, which is labor-intensive and expensive.
In the last few years, the introduction of MALDI-TOF MS into the microbiology laboratory has been revolutionary. In brief, this new technology compares protein profiles by generating spectra based on the m/z ratio.
We evaluated the performance of MALDI-TOF MS for typing serotypes of S. pneumoniae isolates included in the PCV13 vaccine using a machine learning approach. We challenged our classification algorithms with a total of new 215 isolates of S. pneumoniaefrom Argentinian nationwide surveillance.
Our work involved a thorough evaluation and comparison of multiple machine learning models. Through this evaluation, we achieved high accuracy values, indicating the effectiveness of the approach taken in solving the problem. Additionally, we attained high kappa index values, indicating that the predictions made by the models were highly accurate and reliable. We also carefully considered the strengths and limitations of each model. As such, our results are highly valuable and have important implications for future research and applications.
On a cross-diffusive SIS epidemic model with power-like nonlinear incidence
Authors: Li Huicong , Xiang Tian
Published: April 2023

Note: Please see pdf for full abstract with equations.
We study global existence, boundedness and convergence of non-negative classical solutions to a Neumann initial-boundary value problem for the following possibly cross-diffusive SIS (susceptible-infected-susceptible) epidemic model with power-like infection mechanism generalizing the standard mass action incidence:
St = dS∆S + χ∇ · (S∇I) − βSqIp + γI, x ∈ Ω, t > 0,
It = dI∆I + βSqIp − (γ + µ)I, x ∈ Ω, t > 0,
in a bounded smooth domain Ω ⊂ Rn (n ≥ 1). The infection force of the form βSqIp with p, q > 0 is a natural extension of the classical mass action type βSI, and the cross-diffusive term χ∇ · (S∇I) with χ ≥ 0 describes the effect that the susceptible individuals tend to move away from higher concentration of infected populations. Global existence and boundedness of classical solutions are established in certain parameter ranges, and threshold/non-threshold long-time behaviors of global bounded solutions are also detected. Our findings significantly improve and extend previous related studies.
Bioefficacy and persistence of cyantraniliprole in kinnow mandarin under subtropical conditions of Punjab, India
Authors: Pathania Mandeep , Arora Parshotam Kumar
Published: April 2023

Foliar application of cyantraniliprole @ 60 g a.i ha− 1 was found most effective and statistically superior to standard checks imidacloprid and thiamethoxam in reducing Diaphorina citri population in kinnow mandarin upto 14 days of treatment. Thus the nature and amounts of active metabolites of cyantraniliprole used in kinnow are set to ensure the safety of the consumers and environment. Hence residue of cyantraniliprole at effective dose i.e. 60 g a.i ha− 1 and double of effective dose i.e. 120 g a.i ha− 1. using 1000 L of water was estimated from kinnow fruits and soil. The average initial deposit of cyantraniliprole in kinnow fruits were 0.254 and 0.415 mg kg− 1 which dissipated to the extent of 95.28 and 95.15% in the respective doses after 10 days and to below detectable limits after 15 days of spray. The residue of cyantraniliprole in soil was found below detectable limits after 10 days of spray. It is concluded that cyantraniliprole can be used in D. citri management programme in kinnow mandarin and the pre-harvest interval recommended for safe consumption of kinnow fruits was observed to be 15 days.
Breeding habitats, bionomics and phylogenetic analysis of Aedes aegypti and first detection of Culiseta longiareolata, and Ae. hirsutus in Somali Region, eastern Ethiopia
Authors: Yared Solomon , Gebressilasie Araya , Worku Amha , Mohammed Abas , Gunarathna Isuru , Rajamanickam Dhivya , Waymire Elizabeth , Balkew Meshesha , Carter Tamar E.
Published: April 2023

Introduction:
Arboviral diseases, such as dengue, chikungunya, yellow fever, and Zika, are caused by viruses that are transmitted to humans through mosquito bites. However, the status of arbovirus vectors in eastern Ethiopia is unknown. The aim of this study was to investigate distribution, breeding habitat, bionomics and phylogenetic relationship of Aedes aegypti mosquito species in Somali Regional State, Eastern Ethiopia.
Methods
Entomological surveys were conducted in four sites including Jigjiga, Degehabur, Kebridehar and Godey in 2018 (October to December) to study the distribution of Ae. aegypti and a follow up collection was made in 2020 (July-December). In addition, seasonality and bionomics of Ae. aegypti was conducted in 2021 (January-April) in Kebridehar town. Adult mosquitoes were collected from indoor and outdoor locations using CDC light traps (LTs), pyrethrum spray collection (PSCs), and aspirators. Larvae and pupae were also collected from a total of 169 water-holding containers using a dipper between October and November 2020 (rainy season) in Kebridehar town. The species identification of wild caught and reared adults was conducted using a taxonomic key. In addition, species identification using mitochondrial and nuclear genes maximum likelihood-based phylogenetic analysis was performed.
Results
In the 2018 collection, Ae. aegypti was found in all study sites (Jigjiga, Degahabour, Kebridehar and Godey). In the 2020–2021 collection, a total of 470 (Female = 341, Male = 129) wild caught adult Ae. aegypti mosquitoes were collected, mostly during the rainy season with the highest frequency in November (n = 177) while the lowest abundance was in the dry season (n = 14) for both February and March. The majority of Ae. aegypt were caught using PSC (n = 365) followed by CDC LT (n = 102) and least were collected by aspirator from an animal shelter (n = 3). Aedes aegypti larval density was highest in tires (0.97 larvae per dip) followed by cemented cisterns (0.73 larvae per dip) and the Relative Breeding Index (RBI) was 0.87 and Container Index (CI) was 0.56. Genetic analysis of ITS2 and COI revealed one and 18 haplotypes, respectively and phylogenetic analysis confirmed species identification. The 2022 collection revealed no Ae. aegpti, but two previously uncharacterized species to that region. Phylogenetic analysis of these two species revealed their identities as Ae. hirsutus and Culiseta longiareolata.
Conclusion
Data from our study indicate that, Ae. aegypti is present both during the wet and dry seasons due to the availability of breeding habitats, including water containers like cemented cisterns, tires, barrels, and plastic containers. This study emphasizes the necessity of establishing a national entomological surveillance program for Aedes in Somali region.
Search for promising locations to explore geothermal resources based on joint analysis of sections of petro- and thermophysical properties of rocks
Authors: Nenyukova A. I. , Spichak V. V.
Published: April 2023

The purpose of the study is identification of locations promising for geothermal resource exploration based on the joint analysis of sections of petro- and thermophysical properties of rocks. Having conducted magnetotelluric sounding in the geothermal area under investigation and having built 2-D models of electrical resistivity, porosity, permeability, temperature, thermal conductivity and specific heat capacity, the authors performed a joint cluster analysis of sections of enumerated parameters. A cluster cross-section is constructed taking into account all the discussed parameters, which is essentially a petro- and thermophysical “passport” of the site under examination. Each cluster of the latter is characterized by its own set of property ranges. Two promising locations for drilling exploratory boreholes have been identified in the Soultz-sous-Forêts (France) geothermal area. One of them corresponds to the already developed reservoir of petrothermal energy, while another is located at the depths of 2–3 km in another part of the section and has a potential for future exploration works. The information base created on this basis is a convenient tool for interactive selection of sites promising for exploration of different deposits and building of a “prospectivity map” of surface sites for drilling exploration boreholes.
A Path to Protein Quantitation: PBMCs to Western Blot
Authors: Jameel Sufaya , Jahan Parveen , Ramasamy Subbiah
Published: April 2023

Proteins are biopolymers made up of one or more long chains of amino acid residues. These proteins mediate thousands of metabolic pathways that determine how an organism functions. When proteins are thoroughly investigated, they can provide information about health and disease. A variety of methods have been developed to quantify both complex protein mixtures for total protein content as well as a single type of protein. This manuscript covers the methodologies standardized in our lab based on previously published protocols, beginning with the isolation of peripheral blood mononuclear cells (PBMCs) from human blood samples, cell viability testing, cell counting, total protein extraction methods, and protein quantification and estimation techniques, which enable a researcher to find the protocol from PBMCs to Western blot in a single manuscript.
차나무 뿌리에서 분리한 조사포닌의 산화적스트레스 억제 및 미백효과
Authors:   Association for International Tea Culture, Lee Hee-kyung , Choi On-you , Choi DuBok
Published: April 2023

The aim of this research was to investigate the inhibitory effects of oxidative stress, tyrosinase, L-DOPA auto-oxidation, and melanogenesis for whitening effect using tea plant root’s crude saponin. The inhibitory effects of oxidative stress, tyrosinase, L-DOPA auto-oxidation, and melanogenesis increased depending on the concentration of tea plant root's crude saponin. when the concentration of tea plant root’s crude saponin was increased from 50 µg/mL. to 500 µg/mL with SNP 0.3 mM, the survival rate of HepG2 cell was increased from 57.58% to 65.78%, which was about 1.36 fold increase compared negative control without saponin. The maximum inhibitory activity of tyrosinase in Clone-M3 cell was obtained, 61.34% when the tea plant root’s saponin concentrations was 300 µg/mL. The maximum activity of L-DOPA auto-oxidation inhibition was 36.24% at 300 µg/mL. It was confirmed that this plays a major role in the reaction of converting L-tyrosine, which is known to slow down the initial rate determination step, into DOPA in melanin production. The melanin inhibitory activity in Clone-M3 cell with α-MSH were increased from 3.71% to 27.84%, when the crude saponin concentrations were increased from 25µg/mL, to 300µg/mL. Tea plant root’s crude saponin means that it is effective in inhibiting melanin production by inhibiting the activity of tyrosinase proteins in Clone-M3 cells.
A Study on Virtues of Personal Education In Colleges and Personality Traits of Tea Culture
Authors:   Association for International Tea Culture, Choe Hyegyeong
Published: April 2023

Regardless of times and spaces, all countries and societies recognize the values and importance of personality. Whenever unimaginable accidents and happenings occurred, we had to retrospect the current situaion of personal education and as our society gave a focus only on ability, the educational value of personality and its responsibility for society have become important. personality education is engaged in making us human, it should be made for life. It was considered that virtues of personality education had something in common with personality values of tea culture, this study speculated on the correlation. In tea life of tea people of the past, personality values had a great influence on cultivation of their personality. Personlaity training through tea enhanced dignity as tea people. Therefore, as this study investigated the personality traits tea people obtained through tea culture, their views on personality could be understood. Based on the results, this study examined a positive correlation between personality traits of tea culture and personality virues of liberal education in colleges.
Pengaruh Pendidikan Gizi dengan Media Komik Isi Piringku terhadap Sikap dan Asupan Serat pada Anak Gizi Lebih
Authors: Darni Joyeti , Wahyuningsih Retno
Published: April 2023

This study aims to analyze the effect of nutrition education to the attitudes and fiber intake of overweigt children. This was a quasi experimental study with control group using pretest and posttest design involving 60 students that were taken by simple random sampling technique. The subjects were divided into two groups, the first group was given education with comic Isi Piringku and the second group was given education without comic Isi Piringku. The attitude data were measured using an attitude assessment questionnaire. Fiber intake data uses the 2x24 hour food recall method. The attitudes and fiber intake data in two groups were then analyzed by paired T-test. The result showed that the highest attitudes enhancement occurred in the treatment group with a delta of 4.3 points, while the control group was 2.4 points. The average fiber intake before treatment was 11.56 gram and increased to 15 gram after given education using comic Isi Piringku. The results showed that there were significant differences in the enhancement of attitudes and fiber intake before and after nutrition education between the control group and the treatment group with p-value 0.05. Nutrition education using comic Isi Piringku has been proven to be effective in improving children's attitudes and fiber intake. Penelitian ini bertujuan untuk menganalisis pengaruh pemberian edukasi gizi dengan media komik isi piringku terhadap sikap dan asupan serat pada anak gizi lebih. Penelitian ini menggunakan desain quasi experiment dengan control group pretest-posttest. Subjek diambil dengan simple random sampling sebanyak 60 siswa yang dibagi menjadi dua kelompok, yaitu kelompok dengan edukasi komik isi piringku dan kelompok edukasi tanpa komik isi piringku. Data sikap diukur dengan menggunakan kuesioner Data asupan serat diukur dengan menggunakan metode food recall 2x24 jam. Analisis data sikap dan asupan serat pada kedua kelompok diuji menggunakan uji paired T-test. Hasil analisis menunjukan terdapat peningkatan sikap paling tinggi terjadi pada kelompok perlakuan dengan delta 4,3 poin, sedangkan kelompok kontrol sebesar 2,4 poin. Rata-rata asupan serat sebelum perlakuan sebesar 11,56 gram dan mengalami peningkatan setelah pemberian edukasi komik sebesar 15 gram. Hasil uji beda menunjukkan bahwa terdapat perbedaan yang bermakna pada peningkatan sikap dan asupan serat sebelum dan sesudah pemberian edukasi gizi antara kelompok kontrol dan kelompok perlakuan (p 0,05). Pendidikan gizi dengan media komik isi piringku terbukti efektif memperbaiki sikap dan asupan serat anak.
Morfologi Sel-Sel Pulau Langerhans Tikus Putih Sprague Dawley Diabetes Melitus pasca Terapi Fraksi Etil Asetat Sinom Campuran Jeruk Nipis dan Madu
Authors: Wiradnyani Ni Ketut , Yuningsih Dessy Restia , Kusumaningsih Purwaningtyas
Published: April 2023

This study aims to determine changes in the morphology, the number, and diameter of Langerhans Islets cells in White Sprague Dawley rats with diabetes mellitus after treatment of ethyl acetate fraction synom lime and honey mixture. This study is an experimental study which divided into 5 groups, the control group (negative and positive control), the treatment group induced by alloxan and were then given the ethyl acetate fraction of mixed sinom, lime, and honey with a dose of 50; 100; 150; and 200 mg/kg. The histopathological features were observed by HE staining. The results of statistical tests on the average number of cells at a dose of 150 mg/kg BW showed a significant difference (p0.05) from other doses, as well as the observation of the morphology of the islets of Langerhans cells showed the best regeneration of islets of Langerhans in the treatment group with the ethyl acetate fraction of mixed synom drink. Lime and honey are characterized by an increase in the size of the islets of Langerhans, a uniform endocrine cell shape and an almost normal spherical cell nucleus. Penelitian ini bertujuan untuk mengetahui perubahan morfologi sel pulau Langerhans, jumlah, dan diameter pulau Langerhans dari tikus putih Sprague Dawley diabetes melitus pasca terapi fraksi etil asetat sinom campuran jeruk nipis dan madu. Penelitian ini merupakan penelitian eksperimental yang terbagi dalam 5 kelompok, kelompok kontrol (kontrol negatif dan kontrol positif), kelompok perlakuan yang telah diinduksi alloxan kemudian diberikan hasil fraksi etil asetat minuman sinom campuran jeruk nipis dan madu dengan dosis 50; 100; 150; dan 200 mg/kgBB. Gambaran histopatologi diamati dengan pewarnaan HE. Hasil uji statistik rerata jumlah sel dosis 150 mg/kgBB menunjukkan perbedaan nyata (p0,05) dari dosis lainya, demikian juga terhadap pengamatan morfologi sel pulau Langerhans menunjukkan adanya regenerasi sel pulau Langerhans paling baik pada kelompok perlakuan hasil fraksi etil asetat minuman sinom campuran jeruk nipis dan madu ditandai dengan peningkatan ukuran pulau Langerhans, bentuk sel endokrin yang seragam serta intil sel berbentuk bulat hampir mendekati normal.
Изотопные эффекты в спектрах комплексов с водородными связями. Расчет колебательных спектров поглощения димеров (D-=SUB=-2-=/SUB=-CO)-=SUB=-2-=/SUB=- и D-=SUB=-2-=/SUB=-CO·sDF и тримеров D-=SUB=-2-=/SUB=-CO·s(DF)-=SUB=-2-=/SUB=- и (D-=SUB=-2-=/SUB=-CO)_2·sDF
Authors: Булычев В.П. , Бутурлимова М.В. , Тохадзе К.Г.
Published: April 2023

The frequencies and intensities for the vibrational bands of absorption spectra of hydrogen-bonded (D2CO)2 and D2CO∙∙∙DF dimers, two D2CO∙∙∙(DF)2 trimers and four (D2CO)2∙∙∙DF trimers are calculated in the MP2/aug-cc-pVTZ approximation with the basis set superposition error taken into account. Anharmonic values of spectral parameters were obtained using the vibrational second-order perturbation theory. The influence of hydrogen bonds on the spectral parameters was determined from comparison of the values calculated for monomers, dimers, and trimers in the same approximation. The data obtained were compared with the results of previous calculations of (H2CO)2 and H2CO∙∙∙HF dimers and H2CO∙∙∙(HF)2 and (H2CO)2∙∙∙HF trimers. It was shown that one D2CO∙∙∙(DF)2 trimer and two (D2CO)2∙∙∙DF trimers have significant binding energies and strong absorption bands, which makes them promising candidates for detection by spectroscopic methods.
Квантовое измерение амплитуды и фазы электромагнитного поля методом оптического гомодинирования
Authors: Козловский А.В.
Published: April 2023

The possibility of measuring the amplitude and phase of the electromagnetic field by optical light homodyning is shown. A new method for determining the relative phase of the field using the interference scheme of balanced homodyne detection is proposed. The method is based on direct measurement of the average complex amplitude of a quantum field mixed with a classical field using a passive symmetric beam splitter under conditions of a balanced homodyne measurement scheme. A "direct" measurement of the average field amplitude is a direct measurement of the real and imaginary parts of the average quantum mechanical value of the complex field amplitude by homodyning.
By comparison with the quantum theory of the Hermitian phase operator of the electromagnetic field, the accuracy of this measurement is estimated for various quantum states of the microscopic electromagnetic field.
Обработка фотографических спектров фотолюминесценции с помощью компьютерной микрофотометрии на примере кристаллов иодида меди
Authors: Кокин С.М. , Стоюхин С.Г. , Евстропов В.А.
Published: April 2023

The article discusses an approach to the "decoding" of luminescence spectra obtained by the photographic technique by presenting them in the form of graphs of the dependence of the luminescence intensity on the wavelength using a specially developed computer program (computer microphotometry).
Evaluation of Lactobacillus Coryniformis K8 Consumption by Health Care Workers Exposed to COVID-19 (LactoCor2 Project): Protocol for a Randomized Controlled Trial
Authors: Rodríguez-Blanque Raquel , Sánchez-García Juan Carlos , Cobos Vargas Angel , Leyva Martínez M Socorro , Martínez Diz Silvia , Cortés-Martín Jonathan , Tovar-Gálvez María Isabel
Published: April 2023

Background

Lactobacillus coryniformis K8 CECT5711 has immune-modulating properties, enhances the immune response to viral antigens leading to the production of specific antibodies, and has anti-inflammatory activity, which may help to prevent uncontrolled inflammatory processes leading to respiratory and other organ failures.

Objective

The purpose of this study is to evaluate the effect of the consumption of a probiotic strain on the incidence and severity of COVID-19 in health personnel who carry out their professional work among patients with infection or suspected infection by SARS-CoV-2.

Methods

This is a double-blind randomized clinical trial in which the experimental group will receive a capsule of L coryniformis K8 per day (3×109 colony former units/day), and the control group will receive a daily placebo capsule consisting of maltodextrin. A sample size of 314 volunteers was calculated. Volunteers must meet the following inclusion criteria: older than 20 years and active health personnel caring for patients with COVID-19, including all professionals such as medical doctors, nurses, and caretakers at the 2 referral hospitals that treat patients with COVID-19. The main outcome of the clinical trial will be the incidence of symptomatic infection by SARS-CoV-2 in personnel who care for patients with suspected or confirmed COVID-19.

Results

The study had to be extended to the 2 referral hospitals that treat patients with COVID-19 in the province of Granada (Andalusia, Spain); Hospital San Cecilio and Hospital Virgen de las Nieves. A total of 255 individuals met the inclusion criteria and were randomly assigned to one of the 2 groups.

Conclusions

The results of this randomized controlled trial will provide valuable information regarding the administration of L coryniformis K8 against COVID-19, including whether there are fewer infectious processes due to this virus or, in case of occurrence, whether the disease is milder in participants taking the probiotic strain.

Trial Registration

ClinicalTrials.gov NCT04366180; http://www.clinicaltrials.gov/ct2/show/NCT04366180

International Registered Report Identifier (IRRID)

RR1-10.2196/37857
Content Quality of YouTube Videos About Pain Management After Cesarean Birth: Content Analysis
Authors: Squires Natalie A , Soyemi Elizabeth , Yee Lynn M , Birch Eleanor M , Badreldin Nevert
Published: April 2023

Background

YouTube is an increasingly common source of health information; however, the reliability and quality of the information are inadequately understood. Several studies have evaluated YouTube as a resource during pregnancy and found the available information to be of poor quality. Given the increasing attention to postpartum health and the importance of promoting safe opioid use after birth, YouTube may be a source of information for birthing individuals. However, little is known about the available information on YouTube regarding postpartum pain.

Objective

The purpose of this study is to systematically evaluate the quality of YouTube videos as an educational resource for postpartum cesarean pain management.

Methods

A systematic search of YouTube videos was conducted on June 25, 2021, using 36 postpartum cesarean pain management–related keywords, which were identified by clinical experts. The search replicated a default YouTube search via a public account. The first 60 results from each keyword search were reviewed, and unique videos were analyzed. An overall content score was developed based on prior literature and expert opinion to evaluate the video’s relevance and comprehensiveness. The DISCERN instrument, a validated metric to assess consumer health information, was used to evaluate the reliability of video information. Videos with an overall content score of ≥5 and a DISCERN score of ≥39 were classified as high-quality health education resources. Descriptive analysis and intergroup comparisons by video source and quality were conducted.

Results

Of 73 unique videos, video sources included medical videos (n=36, 49%), followed by personal video blogs (vlogs; n=32, 44%), advertisements (n=3, 4%), and media (n=2, 3%). The average overall content score was 3.6 (SD 2.0) out of 9, and the average DISCERN score was 39.2 (SD 8.1) out of 75, indicating low comprehensiveness and fair information reliability, respectively. High-quality videos (n=22, 30%) most frequently addressed overall content regarding pain duration (22/22, 100%), pain types (20/22, 91%), return-to-activity instructions (19/22, 86%), and nonpharmacologic methods for pain control (19/22, 86%). There were differences in the overall content score (P=.02) by video source but not DISCERN score (P=.45). Personal vlogs had the highest overall content score at 4.0 (SD 2.1), followed by medical videos at 3.3 (SD 2.0). Longer video duration and a greater number of comments and likes were significantly correlated with the overall content score, whereas the number of video comments was inversely correlated with the DISCERN score.

Conclusions

Individuals seeking information from YouTube regarding postpartum cesarean pain management are likely to encounter videos that lack adequate comprehensiveness and reliability. Clinicians should counsel patients to exercise caution when using YouTube as a health information resource.
Evolution of Health Information Sharing Between Health Care Organizations: Potential of Nonfungible Tokens (Preprint)
Authors: Esmaeilzadeh Pouyan
Published: April 2023

UNSTRUCTURED

This study attempts to explain the development and progress of the technology used for sharing health information across health care organizations (such as hospitals and physicians’ offices). First, we describe the strengths and weaknesses of traditional sharing models, health information exchange (HIE), and blockchain-based HIE. Second, the potential use of nonfungible token (NFT) protocols in HIE models is proposed as the next possible move for information-sharing initiatives in health care. In addition to some potential opportunities and distinguishing features (eg, ownability, verifiability, and incentivization), we identify the uncertainty and risks associated with the application of NFTs, such as the lack of a dedicated regulatory framework for legal ownership of digital patient data. This paper is among the first to discuss the potential of NFTs in health care. The use of NFTs in HIE networks could generate a new stream of research for future studies. This study provides practical insights into how the technological foundations of information-sharing efforts in health care have developed and diversified from earlier forms.
Efficacy of Mobile-Based Cognitive Behavioral Therapy on Lowering Low-density Lipoprotein Cholesterol Levels in Patients With Atherosclerotic Cardiovascular Disease: Multicenter, Prospective Randomized Controlled Trial (Preprint)
Authors: Li DuanBin , Xu Tian , Xie DaQi , Wang MiaoYun , Sun ShuPing , Wang Min , Zhang SiSi , Yang XinRui , Zhang ZhongNan , Wang Shen , Kuang Ming , Tang Jia , Liu HongYing , Hong XuLin , Fu GuoSheng , Zhang WenBin
Published: April 2023

BACKGROUND

Elevated low-density lipoprotein cholesterol (LDL-C) is an established risk factor for atherosclerotic cardiovascular disease (ASCVD). However, low adherence to medication and lifestyle management has limited the benefits of lowering lipid levels. Cognitive behavioral therapy (CBT) has been proposed as a promising solution.

OBJECTIVE

This trial aimed to evaluate the efficacy of mobile-based CBT interventions in lowering LDL-C levels in patients with ASCVD.

METHODS

This multicenter, prospective, randomized controlled trial enrolled 300 patients with ASCVD, who were randomly assigned to the mobile-based CBT intervention group and the control group in a ratio of 1:1. The intervention group received CBT for ASCVD lifestyle interventions delivered by WeChat MiniApp: “CBT ASCVD.” The control group only received routine health education during each follow-up. The linear regression and logistic regression analyses were used to determine the effects of a mobile-based CBT intervention on LDL-C, triglyceride, C-reactive protein, the score of General Self-Efficacy Scale (GSE), quality of life index (QL-index), and LDL-C up-to-standard rate (<1.8 mmol/L) at the first, third, and sixth months.

RESULTS

Finally, 296 participants completed the 6-month follow-up (CBT group: n=148; control group: n=148). At baseline, the mean LDL-C level was 2.48 (SD 0.90) mmol/L, and the LDL-C up-to-standard rate (<1.8 mmol/L) was 21.3%. Mobile-based CBT intervention significantly increased the reduction of LDL-C change (%) at the 6-month follow-up (β=–10.026, 95% CI –18.111 to –1.940). In addition, this benefit remained when baseline LDL-C <1.8 mmol/L (β=–24.103, 95% CI –43.110 to –5.095). Logistic regression analysis showed that mobile-based CBT intervention moderately increased the LDL-C up-to-standard rates (<1.8 mmol/L) in the sixth month (odds ratio 1.579, 95% CI 0.994-2.508). For GSE and QL-index, mobile-based CBT intervention significantly increased the change of scores (%) at the 1-, 3-, and 6-month follow-up (all P values <.05).

CONCLUSIONS

In patients with ASCVD, mobile-based CBT is effective in reducing LDL-C levels (even for those who already had a standard LDL-C) and can improve self-efficacy and quality of life.

CLINICALTRIAL

Chinese Clinical Trial Registry ChiCTR2100046775; https://www.chictr.org.cn/showproj.aspx?proj=127140