bluuebunny/crossref_metadata_2025 huggingface dataset:
Capacidades estatais para políticas de infraestrutura no Brasil contemporâneo
Authors: Gomide Alexandre de Ávila , Pereira Ana Karine
Published: November 2018

Resumo O texto tem como objetivo discutir as capacidades do Estado brasileiro, notadamente do Poder Executivo Federal, para produzir políticas de infraestrutura eficientes que entreguem os benefícios esperados e obtenham o apoio (ou o consentimento) dos grupos sociais impactados. Argumenta-se que a profissionalização e a qualificação técnica existentes na burocracia pública do governo federal são constrangidas pela baixa autonomia e coesão intragovernamental. Igualmente, as relações da burocracia com a sociedade local, os representantes dos entes federativos e as agências de controle são precárias. Tal situação constitui-se em barreira para a eficiência e legitimidade da ação estatal no setor.
TAXA DE INCIDÊNCIA E O USO DO FLUSHING NA PREVENÇÃO DAS OBSTRUÇÕES DE CATETER VENOSO PERIFÉRICO
Authors: Braga Luciene Muniz , Parreira Pedro Miguel dos Santos Dinis , Arreguy-Sena Cristina , Carlos Diene Monique , Mónico Lisete dos Santos Mendes , Henriques Maria Adriana Pereira
Published: November 2018

RESUMO Objetivo: avaliar a incidência cumulativa de obstrução do cateter venoso periférico e identificar o uso do flushing para prevenção das obstruções. Método: método misto, com estudo de coorte descritivo com seguimento de 110 pacientes de uma clínica médica de Portugal. A coleta de dados envolveu entrevistas com 22 enfermeiros, análise documental e observação participante. Realizada análise temática dos achados qualitativos e análise descritiva para os dados quantitativos. Resultados: a incidência cumulativa de obstrução foi 50%. As categorias temáticas desvelaram que o flushing era um cuidado para prevenção da obstrução do cateter venoso e realizado antes e/ou após a administração dos medicamentos. O volume de solução fisiológica utilizado no flushing variou entre 3 e 10 ml. Verificaram-se, também, situações de não adesão ao flushing e fatores que influenciavam nesta adesão, a saber: o tempo para realizar os cuidados, a complexidade e o grau de dependência dos pacientes, o volume de trabalho e o número de enfermeiros para prestar os cuidados. Conclusão: o flushing é um cuidado de enfermagem para a prevenção da obstrução do cateter venoso periférico, no entanto, as práticas de enfermagem para implementação não são uniformes quanto a frequência e volume de solução fisiológica. A ausência de um protocolo de enfermagem, a complexidade e o grau de dependência dos pacientes, o volume de trabalho e o número de enfermeiros são fatores capazes de influenciar na adesão à prática do flushing e consequentemente na incidência de obstrução do cateter venoso periférico e na segurança do paciente e qualidade dos cuidados.
TEORIA FUNDAMENTADA NOS DADOS EM TESES E DISSERTAÇÕES DA ENFERMAGEM BRASILEIRA
Authors: Silva Glauber Weder dos Santos , Enders Bertha Cruz , Sousa Francisca Georgina Macedo de , Sena Julliana Fernandes de , Santos Renata Clemente dos , Silva Amanda Barbosa da
Published: November 2018

RESUMO Objetivo: identificar como os pesquisadores utilizaram o método da Teoria Fundamentada nos Dados nas teses e dissertações da enfermagem brasileira. Método: trata-se de estudo documental com abordagem quantitativa. Como fonte de coleta de dados, foram utilizados os relatórios depositados no Catálogo de Teses e Dissertações do Centro de Estudos e Pesquisas em Enfermagem da Associação Brasileira de Enfermagem, entre 2001 e 2015, com posterior resgate da versão completa no repositório da instituição de ensino superior de origem das teses e dissertações. A coleta ocorreu a partir de extração dos dados em planilhas que foram submetidos à analise descritiva. Resultados: elegeram-se 70 teses e dissertações (0,95%) do total de 7.369 estudos. A maioria (70%) descreveu como foram construídos os grupos amostrais. Somente 4,3% não registrou ter realizado a coleta e analise dos dados de forma concomitante. Dos estudos que descreveram todas as etapas, 34,3% não apresentaram o processo de validação do modelo teórico. Os diagramas e os memorandos foram utilizados, respectivamente, em 74,3% e 65,7% das teses e dissertações analisadas. O referencial teórico mais frequente foi o Interacionismo Simbólico e a perspectiva metodológica foi a Straussiana. Evidenciou-se que para os pesquisadores, a entrevista foi a principal técnica de coleta utiliza de forma isolada e combinada com outras abordagens. Conclusão: a diversidade na apresentação e na descrição do método e resultados dos estudos revela que ainda há necessidade de aprimoramento quanto ao uso do método, considerando as diferentes vertentes da teoria fundamentada nos dados.
NEUROMUSCULAR ELECTRICAL STIMULATION OF MEDIUM AND LOW FREQUENCY ON THE QUADRICEPS FEMORIS
Authors: Pinfildi Carlos Eduardo , Andraus Rodrigo Antonio Carvalho , Iida Ligia Megumi , Prado Rodrigo Paschoal
Published: November 2018

ABSTRACT Objective: The purpose of this study was to investigate the effects of neuromuscular electrical stimulation (NMES) in different frequencies on the quadriceps femoris. A randomized, controlled, blind cross-sectional study. Methods: Thirty subjects (12 men and 18 women), with an average age of 24.67 years, weight of 65.62 kg and height of 1.69 m were evaluated. Three random test conditions were applied: maximum voluntary isometric contraction (MVIC), maximum voluntary isometric contraction with medium frequency current (MVIC-MF) and maximum voluntary isometric contraction with low frequency current (MVIC-LF). Four MVICs were applied in each situation. The time between different isometric contraction types was 90 seconds while the time between the same conditions of contraction was 10 seconds. Results: Two-way ANOVA test showed that MVIC-MF had higher values for peak torque than MVIC-LF (p=0.02). Significant statistical results were found when comparing MVIC-MF and MVIC (p=0.03), but not for MVIC and MVIC-LF (p=0.52). Conclusion: Maximum voluntary isometric contraction associated with medium-frequency electrical stimulation was more effective than other NMES conditions. Level of Evidence II, Therapeutic Studies - Investigation of treatment results.
INCISIONAL NEGATIVE-PRESSURE WOUND THERAPY IN REVISION TOTAL HIP ARTHROPLASTY DUE TO INFECTION
Authors: Miyahara Helder de Souza , Serzedello Felipe Ribeiro , Ejnisman Leandro , Lima Ana Lúcia Lei Munhoz , Vicente José Ricardo Negreiros , Helito Camilo Partezani
Published: November 2018

ABSTRACT Objective: To present our institution's experience with negative-pressure wound therapy (NPWT) as an adjuvant in wound healing of patients who have undergone revision total hip arthroplasty (THA) due to septic loosening in the presence of active fistula. Methods: We prospectively assessed patients presenting with THA infection, associated with the presence of fistula, treated with a PICO® device for NPWT, in combination with the standard treatment for prosthesis infection in our institution. Resolution of the infectious process and healing of the surgical wound without complications were considered an initial favorable outcome. Results: We assessed 10 patients who used PICO® in our department. No complications were identified in association with the use of the NPWT device. The mean follow-up of the patients after use of the device was 12.7 months. Only one patient progressed with fistula reactivation and recurrence of infection. Conclusion: NPWT can be used in wound complications and infection following THA procedures safely and with promising results. Randomized prospective studies should be conducted to confirm its effectiveness. Level of Evidence IV, Case Series.
Testicular histological evaluation and serum testosterone concentrations of bulls after chemical castration with calcium chloride
Authors: Pereira Lucas F. , Dias Fernanda G.G. , Miguel Marina P. , Honsho Cristiane S. , Tavares Denise C. , Hellú José A.A. , Souza Fabiana F.
Published: November 2018

ABSTRACT: Orchiectomized bulls have advantages in the meat quality and ease of handling. Chemical castration is an option for surgical castration and the sclerosing agents can be administered into the testicular or epididymis parenchyma. These agents have a lower incidence of complications than surgery, especially when associated with dimethylsulfoxide (DMSO), which has anti-inflammatory action and increases the absorption of other drugs. Thus, this study aimed to evaluate the effect of a single intratesticular injection of calcium chloride solution associated with DMSO for the chemical sterilization of bulls. Twenty-four young adult bulls were utilized, distributed into 3 groups (G20, G30 and G40, n = 8/group), according to the calcium chloride concentration (20, 30 and 40%), in 10mL volume. Serum concentrations of testosterone, body weight, testicular volume and ecotexture, clinical signs and behavior and were evaluated for 45 days. Thus, the animals were orchiectomized and testicles were assessed histologically. There were no changes in body weight, decreased serum testosterone concentrations (except G30), signs of scrotal sensitivity or changes in behavior over the period. However, there was significant increase in testicular volume, especially on the 2nd and 3rd day after treatment, with values returning to the value initials at 15 days. Testicular adherence and firm consistency were observed during orchiectomy. Ultrasound examination revealed a loss of integrity of the median raphe, with cavity formation and an alteration of the testicular echotexture. In the histological evaluation, coagulation necrosis of seminiferous tubules and interstitial cells was observed, mainly in the medial portion in all groups. Some animals presented total absence of tubular formations in all the studied groups, being the effects of greater intensity in the G40. Additionally, pronounced edema was noted in all groups, especially in G40. Inflammatory infiltrate, fibroplasia and neovascularization were found to be predominantly discrete. Based on the conditions used in this study, we conclude that calcium chloride associated with DMSO can be used as a method of chemical sterilization in bovines.
Determination of the reference interval for the activity of N-Acetyl-β-D-Glucosaminidase (NAG) in urine of healthy dogs
Authors: Tassini Luiz Eduardo S. , Leme Fabiola O.P. , Veado Júlio César C. , Arndt Márcio H.L.
Published: November 2018

ABSTRACT: The diagnosis of acute kidney injury by evaluating the activity of urinary enzymes is an important tool especially for its precocity in relation to methods of assessment of renal function considered late as the installation of injury precedes the function change. This study was performed to determine the reference interval for urinary NAG activity (N-Acetyl-β-D-glucosaminidase) in urine of healthy dogs of different breeds, ages and sexes. It was utilized an automated method for the determination of NAG activity standardized for dog urine samples. Through statistical analysis it was observed that NAG is positively correlated with the age of the animal and urine specific gravity but is not interfered by sex or weight. Based on the determination of urinary activity and after correcting the values for a 1,025 urine specific gravity was obtained an reference interval of X ‘= 3.62U/L±0.66U/L.
In vitro and in vivo efficacy of tea tree essential oil for bacterial and yeast ear infections in dogs
Authors: Neves Rita C.S.M. , Makino Hérica , Cruz Thalita P.P.S. , Silveira Marcelo M. , Sousa Valéria R.F. , Dutra Valéria , Lima Marcia E.K.M. , Belli Carla B.
Published: November 2018

ABSTRACT: Otitis externa is a common complaint in dogs. Bacteria and yeasts are commonly involved and may perpetuate inflammatory reactions inside the ear canal. Otoscopy, cytological examination of secretion and microbiological culture embody forms of diagnosis. Cytology also has great use in accessing treatment evolution. Therapy usually consists of cleaning ear canals and subsequent use of antibiotics or antifungal products. As some of them may cause hypersensitivity and even ototoxicity, searching for new pharmacological bases is currently necessary and justifies this study, which aimed to evaluate in vitro and in vivo efficacy of tea tree essential oil for bacterial and yeast ear infections in dogs. Twenty-eight dogs from a particular shelter in Cuiabá (Mato Grosso, Brazil), presenting clinical signs of otitis externa, were enrolled in this clinical trial. In all of them, clinical and cytological evaluations, as well as culture and susceptibility testing of the affected ears were carried out. From each dog, one ear was treated with 5% tea tree essential oil lotion and the other with standard otic formulation, according to the type of infection (bacterial, yeast or both). In vitro susceptibility testings of all ear cultures, to the same drugs used in treatment, were also carried out. Culture results showed 62.5% bacterial and fungal infection, 33.9% bacterial infection and 3.6% fungal infection, from the 56 ear samples collected. The most common microorganisms isolated were Staphylococcus intermedius, Staphylococcus aureus, Proteus mirabilis and Malassezia pachydermatis. Gram-positive bacteria were susceptible to gentamycin in 60.5% and resistant in 16.3% of the samples. Five percent tea tree essential oil formulation produced a 5mm clear zone of inhibition around the disks in one of the 63 samples evaluated. Pure (100%) tea tree essential oil formulation produced a 10mm clear zone of inhibition around the disks in four of the 63 samples evaluated, a 9mm zone in three samples, an 8mm zone in 16 samples, a 7mm zone in seven samples, a 6mm zone in two samples and there was no clear zone in 31 samples. Inhibition zones were produced by strains of Staphylococcus intermedius, Staphylococcus hyicus, Corynebacterium sp., Proteus mirabilis and Enterobacter sp. tea tree essential oil ear solution significantly induced remission of clinical signs both in bacterial and yeast ear infections. It also reduced as much Malassezia pachydermatis ear infection as the nystatin solution used in this study, while gentamycin solution showed better antibacterial effect. More studies should be conducted to evaluate in vitro diffusion properties of tea tree essential oil. Good antimicrobial spectrum and the absence of adverse reactions confirm the importance of developing a tea tree formulation as an alternative therapy for ear infections in dogs.
Occurrence of brucellosis, leptospirosis and neosporosis in cows with retained placenta in Southwest Paraná, Brazil
Authors: Porto Yuri F. , Pinto Neto Adalgiza , Bernardi Fabrício , Possa Marina Gabriela , Mota Marcelo F. , Martinez Antonio C. , Merlini Luiz Sérgio , Berber Rodolfo C.
Published: November 2018

ABSTRACT: The aim of this study was to measure the occurrence of brucellosis, leptospirosis and neosporosis in cows from 25 family farms milk, with semi-intensive grazing system, located in the Southwest region of Paraná, Brazil. Eighty-four cows with retained placenta, between July 2013 to July 2014, diagnosed by the presence of fetal membranes in the uterus up to 12 hours after partum, decrease of appetite and of milk production, as well increase of rectal temperature were included into the study. The animals were submitted to blood collection for evaluating the seropositivity for brucellosis, leptospirosis and neosporosis. The blood was collected at 15 to 45 days postpartum, avoiding false negative results due to immune deficiency observed in the transition period. After collection, the blood was centrifuged, the serum was packed in three aliquots, identified and subsequently frozen. Serology was performed to diagnose brucellosis (technique of buffered acidified antigen), leptospirosis (through the microscopic agglutination test - MAT) and neosporosis (indirect immunofluorescence test). Animals were considered positive when presenting titer >1:100 (leptospirosis) and 1:200 (neosporosis). No animal with placenta retention presented Brucella abortus bacteria; 39% (33/84) were reactive to one or more Leptospira serovars, 15% (13/84) were positive to Neospora caninum, and 4% (4/84) had both diagnosis, being reactive for leptospirosis and neosporosis. In conclusion, data from this experiment inspires greater attention to leptospirosis and neosporosis in dairy cattle presenting placenta retention in southwest Paraná.
Assessment of the vertebral heart scale and cardiothoracic ratio to standardize the heart size of collared peccaries (Tayassu tajacu Linnaeus, 1758) restrained with ketamine and midazolam
Authors: Silva-Filho Osmar F. , Pessoa Gerson T. , Sousa Francisco C.A. , Rodrigues Renan P.S. , Moura Laecio S. , Ambrósio Carlos E. , Silva Andrezza B.S. , Alves Flávio R.
Published: November 2018

ABSTRACT: Wild species, especially those threatened with extinction, are increasingly being investigated to obtain information that can be useful for their preservation. The objective of the present study was to standardize the vertebral heart scale (VHS) and cardiothoracic ratio (CTR) of the collared peccary (Tayassu tajacu Linnaeus, 1758) sedated with ketamine and midazolam. Fourteen clinically healthy collared peccaries were examined in the two-year age group weighing 15-22kg. The animals were submitted to digital radiography of the thorax in lateral and dorsal ventral projections to calculate the VHS and CTR. The VHS mean values for males and females was 8.88±0.51v for right recumbency and 8.84±0.39v for left decubitus, and there were no significant between-gender differences regarding recumbency (p>0.05). The CTR showed mean values of 0.50±0.05 (males) and 0.45±0.04 (females), but the gender-differences were not significant (p>0.05). A positive correlation was shown between VHS and CTR (r=0.98, right decubitus; r=0.96, left decubitus). Establishing reference values for heart measurements in collared peccaries using digital radiography of the thorax permitted standardization of the VHS and CTR values for this wild species. In the studied wild animal model, the VHS and CTR heart assessment indexes were shown to be essential diagnostic tools for investigations of alterations in the size of the cardiac silhouette.
Iron supplementation for lambs experimentally infected by Haemonchus contortus: response to anemia and iron store in the bone marrow
Authors: Casanova Valeska P. , Aires Adelina R. , Collet Silvana G. , Krause Alexandre , Moresco Rafael N. , Bochi Guilherme V. , Silva Aleksandro S. , Leal Marta L.R.
Published: November 2018

ABSTRACT: To evaluate the effect of supplementation of iron dextran on blood variables and iron metabolism in lambs experimentally infected by Haemonchus contortus, four experimental groups were used: uninfected and non-supplemented animals (GI); infected animals supplemented with iron (GII); uninfected animals supplemented with iron (GIII); and infected non-supplemented animals (GIV). Groups II and IV received 10,000 larvae (L3) of Haemonchus contortus, and groups II and III received three doses of iron dextran (20mg/kg) intramuscularly with seven days of interval. Blood and faeces samples were collected on days 10 (D10), 17 (D17), 24 (D24), and 31 (D31), in order to determine red blood cell counts, iron metabolism, and EPG. Infected animals developed anemia from D24 and anemia was more severe on D31. Animals from GII had higher hematocrit and hemoglobin concentration compared to animals of GIV on D31. Iron stores in the bone marrow were higher in GII and GIII compared to GI and GIV. The GIV showed lower seric levels of iron on D24 compared to the other groups. The iron supplementation reduces the severity of the anemia caused by infection with Haemonchus contortus in lambs, improving erythropoietic response after blood loss.
Proteolytic and lipolytic potential of Pseudomonas spp. from goat and bovine raw milk
Authors: Ribeiro Júnior José C. , Teider Junior Pedro I. , Oliveira André L.M. , Rios Edson A. , Tamanini Ronaldo , Beloti Vanerli
Published: November 2018

ABSTRACT: Pseudomonas, the main genus of gram-negative microorganisms isolated from milk, is psychrotrophic, biofilm-forming, and thermo-resistant deteriorating enzyme producers. The aim of this study was to quantify Pseudomonas spp. in goat’s and cow’s milk produced in the Paraná state, Brazil, to evaluate the deteriorating activity of the isolates at mesophilic and psychrotrophic conditions and to identify, at the species level, the isolates with alkaline metalloprotease (aprX gene) production potential. Microbiological, biochemical and molecular methods were used for isolating, confirming and identifying of isolates. The mean counts were 1.6 (±6.3)x104 and 0.89(±3)x102 CFU/mL for goat and bovine milk samples, respectively, immediately after milking. Of the Pseudomonas colonies isolated from goat milk (n=60), 91.7% showed proteolytic potential when incubated at 35°C/48 h and 80% at 7°C/10 days, and lipolytic potential was observed in 95% of the isolates incubated in mesophilic and 78.3% at refrigeration conditions. From the isolates of bovine milk (n=20), 35% showed proteolytic activity only when incubated at 35°C/48 h, and lipolytic potential was observed in 25% of the isolates incubated at 7°C/10d and 35°C/48h. It was observed that 83.3% and 25% of the isolates genetically confirmed as Pseudomonas spp. of goat and bovine milk showed the potential for alkaline metalloprotease production, with the species P. azotoformans, P. koreensis, P. gessardii, P. monteilii and P. lurida being the most frequent in goat milk and P. aeruginosa the only species identified in cow milk.
An outbreak of malignant catarrhal fever in Sambar deer (Rusa unicolor)
Authors: Oliveira Mariana C. , Pereira Gabriela O. , Daoualibi Yasmin , Dutra Valéria , Brito Marilene F. , Caldas Saulo A. , Balthazar Daniel A. , Ubiali Daniel G.
Published: November 2018

ABSTRACT: Malignant catarrhal fever (MCF) is an infectious, pansystemic and highly fatal disease with wide geographic distribution. The species that are clinically prone to it include cattle, deer and bison. In Brazil, the disease in ruminants and deer is associated with the contact with sheep, especially during labor, when the fetal remains that are eliminated contain the ovine herpesvirus 2 (OvHV-2). The outbreak took place in a conservationist property in the city of Casimiro de Abreu/RJ, which hosted 23 Sambar deer, and, of these, 19 died, showing neurological signs. The deer lived in a location together with 15 male and female meat sheep. A female specimen of the Sambar deer (Rusa unicolor), aged approximately three years, which had presented with neurological clinical signs was referred to necropsy in the Setor de Anatomia Patológica at Universidade Federal Rural do Rio de Janeiro (SAP/UFRRJ). During necropsy, cerebrospinal fluid was sampled for analysis; fragments of several organs were fixated in 10% buffered formalin and processed for histopathological analysis. Fragments of occipital lobe, cerebellum and bulb were collected to perform the polymerase chain reaction (PCR). The diagnosis of this outbreak was based on epidemiological, clinical and pathological findings, and on the amplification of the OvHV-2 DNA through PCR. The histological changes were the base to confirm the MCF case and were characterized by degeneration of vascular endothelial cells, fibrinoid vasculitis, hyperplasia and necrosis of lymphoid organs. However, PCR was an important tool to confirm the diagnosis. MCF as an important disease with nervous symptomatology in deer.
Poisoning of cattle by Senecio spp. in Brazil: a review
Authors: Panziera Welden , Pavarini Saulo P. , Sonne Luciana , Barros Claudio S.L. , Driemeier David
Published: November 2018

ABSTRACT: Poisoning of cattle by plants of the Senecio genus represents an important cause of death in cattle and has important economic repercussions in southern Brazil. This review is intended to provide a detailed review of Senecio spp. intoxication in cattle and addresses issues regarding the toxic principle and pathogenesis of the disease caused by these plants and the epidemiology, clinical signs, diagnosis, control and prophylaxis of the disease. Senecio brasiliensis is the main species associated with natural intoxication of livestock in Brazil, and the number of cases associated with the ingestion of S. madagascariensis is increasing. The toxic principle of Senecio spp. comprises the hepatotoxic alkaloids of the pyrrolizidine group (pyrrolizidine alkaloids, PAs). The resulting liver lesions are chronic and irreversible and result from the inhibition of hepatocellular mitosis. Deaths of adult cattle may occur both sporadically and in larger outbreaks over an extended period of time. In cattle raising, Senecio spp. are consumed mainly during the winter, when there is a dearth of forage; at this time the poisonous Senecio species are budding and contain high concentrations of PAs. Spontaneous Senecio spp. intoxication in cattle is a chronic condition that frequently involves acute clinical manifestations. Affected cattle may present ascites, emaciation, intermittent dark diarrhea, tenesmus, rectal prolapse, and neurological signs resulting from hepatic encephalopathy. Hepatogenous photosensitization may be observed but is uncommon. Necropsy findings include dependent subcutaneous edema, ascites, and edema of the mesentery, abomasal folds, and gallbladder. The liver is firm, normal or reduced in size with a white, thick capsule. Microscopically, the hallmarks of Senecio-associated disease are varying degrees of hepatocellular megalocytosis, bile duct hyperplasia and fibrosis. Sheep are significantly more resistant to Senecio intoxication than are cattle and avidly ingest Senecio plants; therefore, the use of sheep for grazing infested pasture is recommended for preventing the associated disease in cattle.
Modeling conditional volatility by incorporating non-regular trading hours into the APARCH model
Authors: Araújo Breno Valente Fontes , Camargos Marcos Antônio de , Pinho Frank Magalhães de
Published: November 2018

ABSTRACT This study aims to evaluate how the after-market and pre-opening periods affect the estimation of conditional volatility one day ahead. Volatility features quite a lot in Finance studies because it is a fundamental parameter in derivatives pricing, the efficient allocation of portfolios, and risk management. The results are relevant for investment agents to be able to refine volatility forecasting models and achieve better results in derivatives pricing, risk management, and portfolio optimization. We used the asymmetric power autoregressive conditional heteroscedasticity (APARCH) model, incorporating the after-market, pre-opening, and total overnight periods to assess whether they contain important information for modeling volatility. We analyzed the 20 stocks of Brazilian companies listed on the São Paulo Stock, Commodities, and Futures Exchange (BM&FBovespa) and also belonging to the BR Titans 20 with ADRs listed on the New York Stock Exchange and the Nasdaq. The results were evaluated in-sample using the corrected Akaike information criterion (AICc) and the statistical significance of the coefficients, and out-of-sample using root mean squared error (RMSE), mean absolut percentage error (MAPE), the R² of the Mincer-Zarnowitz regression, and the Diebold Mariano test. The analysis does not enable it to be claimed which is the best model, because there is no unanimity among all the stocks; however, non-regular trading hours were shown to incorporate important information for most of the stocks. Furthermore, the models that incorporated the pre-opening period generally obtained superior results to the models that incorporated the after-market period, demonstrating that this period contains important information for forecasting conditional volatility.
Bacillus cereus group: genetic aspects related to food safety and dairy processing
Authors: Rossi Gabriel Augusto Marques , Aguilar Carlos Eduardo Gamero , Silva Higor Oliveira , Vidal Ana Maria Centola
Published: November 2018

ABSTRACT: Bacillus cereus group includes not pathogenic and high pathogenic species. They are considered as a risk to public health due to foodborne diseases and as an important cause of economic losses to industries due to production of spoilage enzymes. Some researches have been performed in order to assess the possible factors that contribute to put public health into risk because of consumption of food contaminated with viable cells or toxins which have complex mechanisms of production. The control of these bacteria in food is difficult because they are resistant to several processes used in industries. Thus, in this way, this review focused on highlighting the risk due to toxins production by bacteria from B. cereus group in food and the consequences for food safety and dairy industries.
Pathogenicity and transmission of fungi detected on Passiflora alata seeds
Authors: Parisi João José , Fischer Ivan Herman , Medina Priscila Fratin , Firmino Ana Carolina , Meletti Laura Molina
Published: November 2018

ABSTRACT: Passion fruit is usually propagated by seeds because of the ease and lower cost in seedling production. However, the seed is the most efficient agent for the spread of pathogens. The damages from seed-borne diseases occur mainly during the germination stages or at the formation of seedlings in nurseries. Considering the need for knowledge on the pathology of sweet passion fruit seeds, the objective was to evaluate the transmission and pathogenicity of the fungi Alternaria sp., Botrytis fabae, Cladosporium cladosporioides, Fusarium spp. and Lasiodiplodia theobromae, known as potentially pathogenic to this crop, and isolated from sweet passion fruit seeds. Therefore, tests on seed health, germination and seedling emergence in a sterilized commercial substrate were conducted using seeds from this species, inoculated with those fungal isolates. Leaves, stems and fruit from this plant were also inoculated with the same fungi. Alternaria sp., Fusarium spp. and L. theobromae were identified in seedlings obtained from inoculated seeds, confirming the transmission of these fungi by seeds. L. theobromae was also considered the most harmful fungus to passion fruit crop, as it causes seed rot and other disease symptoms on the leaves, stem and fruit. These findings inferred that healthy seeds of sweet passion fruit are essential for producing seedlings and to prevent the spread of the diseases caused by these fungi to exempt areas.
EFEITO DA HERANÇA DA MARCA NOS RELACIONAMENTOS ENTRE CONSUMIDORES E MARCAS
Authors: FRIZZO FRANCIELLE , KORELO JOSÉ CARLOS , PRADO PAULO HENRIQUE MÜLLER
Published: November 2018

RESUMO A herança é uma proposição de valor da marca que fornece uma base única para criar e manter relações fortes com os consumidores. Buscando compreender como esse recurso estratégico influencia as relações entre consumidores e marcas, este estudo objetiva examinar a herança da marca como determinante dos elementos de reforço do self (seduzindo, habilitando e enriquecendo o self). Uma pesquisa com 309 consumidores brasileiros e americanos foi realizada para testar as relações propostas. Baseados em um modelo de equação estrutural, os resultados da pesquisa demonstraram que os consumidores processam as características relacionadas à herança da marca por meio dos três elementos de reforço do self, mas seu efeito global na conexão self-marca só ocorre por meio do prazer sensorial e estético que a marca oferece (seduzindo o self). O estudo é concluído apresentando as implicações acadêmicas e gerenciais e sugerindo pesquisas futuras.
IMPACTO DOS E-BOOKS NA CADEIA EDITORIAL BRASILEIRA: UMA ANÁLISE EXPLORATÓRIA
Authors: FERREIRA FERNANDO COELHO MARTINS , MIRANDA LUIS FERNANDO MOREIRA , MORAS MONIQUE
Published: November 2018

RESUMO Este trabalho tem como objetivo analisar os principais efeitos do surgimento dos livros eletrônicos na configuração da cadeia editorial brasileira. Entrevistas com executivos de grupos editoriais instalados no País permitiram identificar que os e-books ainda desempenham um papel secundário na cadeia editorial, tendo maiores chances de prosperar em alguns segmentos específicos de livros. Os livros eletrônicos não tornaram essa cadeia mais enxuta. Uma vez que os e-books não substituíram os livros físicos, mas passaram a coexistir com estes, coube às editoras desenvolver um novo conjunto de atividades dentro da cadeia editorial existente. A desintermediação é uma das características mais marcantes desse novo processo produtivo. Para se adequar a esse novo cenário, o setor editorial necessita de ajustes que vão além de suas operações, implicando também a redefinição dos recursos e competências que precisa desenvolver.
The experience of the partners of women with breast cancer
Authors: Yoshimochi Leonardo Toshiaki Borges , Santos Manoel Antônio dos , Loyola Edilaine Assunção Caetano de , Magalhães Paola Alexandria Pinto de , Panobianco Marislei Sanches
Published: November 2018

ABSTRACT Objective: To understand the experience of partners of women with breast cancer. Method: This was a qualitative study, based on the theoretical-methodological framework of existential and phenomenological psychology, and carried out with the partners of women with breast cancer who attended a center for rehabilitation after mastectomy. The data were collected in interviews between June and November 2014, based on a guiding question. Results: Ten partners participated in the study. On discovering the disease, the partners experienced emotional distress caused by the fear of losing their partners. Before this suffering, they demonstrated the ability to react: willingness to provide care, to comfort and to support the women in search of treatment, and together, seek to reverse adverse situations. They also showed sensitivity and recognized the intense emotional suffering of the women caused by the challenges faced throughout the course of treatment. Conclusion: The present study showed the intense suffering of the partners of women with breast cancer and their difficulty to ask for help. Thus, health professionals must be alert, addressing their needs throughout the treatment process to mitigate suffering.
Battersea cable tunnel: powering regeneration in central London, UK.
Authors: Quigg Gerard
Published: November 2018

A new primary electrical substation is being provided to support regeneration of the Battersea Power Station and surrounding Nine Elms area in central London. Due for completion in 2019, it required tunnelling 330 m under rail tracks to connect to an existing 132 kV cable tunnel. As described in this paper, the new spur tunnel was constructed using an open-face tunnelling shield beneath rail viaducts and a Victorian sewer. Through careful sequencing and close monitoring, a volume loss of just 1% was achieved during construction of the complex junction chamber connection with the existing wedge-block tunnel.
SUBCRITICALITY ANALYSIS OF HTR-10 SPENT FUEL CASK MODEL FOR THE 10 MW HTR INDONESIAN EXPERIMENTAL POWER REACTOR
Authors: Sembiring Tagor Malem , Artiani Pungky Ayu
Published: November 2018

The 10 MW HTR Indonesian Experimental Power Reactor (RDE reactor) is designed identical with the HTR-10 in China, conceptually.  However, the review results showed that the spent fuel cask model which is used between two reactors is fully different, such as size and capacity. The proposed cask model in RDE reactor can hold 15 times more fuel pebbles than HTR-10 has.  This research activities deal with the subcriticality analysis for the spent fuel cask of RDE reactor if using the HTR-10 cask model.  The subcriticality condition is designed to meet the limit of safety value.  The objective of this research is to determine the subcriticality value in the normal and accident events for the spent fuel cask when it is in the reactor building and the spent fuel cask room.  All calculations were carried out by MCNP6.1 code.  The selected external events are the water ingress (reactor room), water flood and the combination event of water flood and earthquake.  The calculation results showed that the maximum value of keff (3σ) are  0.47510 and 0.19214 for the cask in the reactor building and in the spent fuel cask room, respectively.  This value is far from the limit value of 0.95. The calculation results showed that the spent fuel cask are in the safe condition eventhough in the worst combination events, the cask is flooded and earthquake. The HTR-10 spent fuel cask can be proposed as an alternative for the RDE reactor to get an efficient reactor building.Keywords: spent pebble fuel element, HTGR, subcriticality, MCNP6.1, RDE reactor ANALISIS SUBKRITIKALITAS PENYIMPAN BAHAN BAKAR BEKAS MODEL CASK REAKTOR HTR-10 UNTUK REAKTOR DAYA EKSPERIMENTAL 10 MW TERMAL. Reaktor Daya Eksperimental (RDE) secara konseptual didesain identik dengan reaktor HTR-10 di Tiongkok.  Meskipun demikian, terdapat perbedaan yang signifikan untuk desain konseptual cask penyimpan bahan bakar bekas di kedua reaktor seperti dimensi dan kapasitas.  Kegiatan penelitian ini berkaitan dengan analisis subkritikalitas cask penyimpan elemen bahan bakar bekas tipe pebble di RDE jika menggunakan model cask yang dipakai di HTR-10. Kondisi sub-kritikalitas didesain memenuhi nilai batas keselamatan. Tujuan penelitian adalah menentukan nilai subkritikalitas dalam keadaan normal atau kondisi kecelakaan di gedung reaktor dan di gudang penyimpan bahan bakar bekas.  Perhitungan dilakukan dengan paket program MCNP6.1. Kejadian kecelakaan yang dipilih adalah masuknya air ke dalam cask, cask terendam air dan kombinasi cask terendam air dan kejadian gempa. Hasil perhitungan menunjukkan bahwa nilai maksimum keff (3σ) untuk cask di gedung reaktor dan di gudang penyimpan bahan bakar bekas masing-masing adalah 0,47510 dan 0,19214.  Nilai ini masih jauh dari batas 0,95.  Hasil perhitungan menunjukkan bahwa cask penyimpan bahan bakar bekas tetap dalam keadaan selamat meski terjadi kombinasi 2 kejadian eksternal.Kata kunci: elemen bahan bakar bekas tipe pebble, HTGR, subkritikalitas, MCNP6.1, RDE
VALIDATION OF PWR-FUEL CODE FOR STATIC PARAMETERS IN THE LWR CORE BENCHMARK
Authors: Kuntoro Iman , Pinem Surian , Sembiring Tagor Malem
Published: November 2018

The PWR-FUEL code is a multi dimensional, multi group diffusion code with nodal and finite difference methods. The code will be used to calculate the fuel management of PWR reactor core. The result depends on the accuracy of the codes in producing the core effective multiplication factor and power density distribution. The objective of this research is to validate the PWR-FUEL code for those cases. The validation are carried out by benchmarking cores of IAEA-2D, KOERBERG-2D and BIBLIS-2D. The all three cases have different characteristics, thus it will result in a good accuracy benchmarking. The calculation results of effective multiplication factor have a maximum difference of 0.014 %, which is greater than the reference values. For the power peaking factor, the maximum deviation is 1.75 % as compared to the reference values. Those results show that the accuracy of PWR-FUEL in calculating the static parameter of PWR reactor benchmarks are very satisfactory.Keywords: Validation, PWR-FUEL code, static parameter. VALIDASI PROGRAM PWR-FUEL UNTUK PARAMETER STATIK PADA TERAS BENCHMARK LWR. Program PWR-FUEL adalah program difusi multi-dimensi, multi-kelompok dengan metode nodal dan metode beda hingga. Program ini akan digunakan untuk menghitung manajemen bahan bakar teras reaktor PWR. Akurasi manajemen bahan bakar teras PWR tergantung pada akurasi program dalam memprediksi faktor multiplikasi efektif teras dan distribusi rapat daya. Untuk itu dilakukan validasi program PWR-FUEL sebagai tujuan dalam penelitian ini.  Validasi PWR-FUEL dilakukan menggunakan teras benchmark IAEA-2D, KOERBERG-2D dan BIBLIS-2D. Ketiga kasus ini mempunyai karaktristik yang berbeda sehingga akan memberikan hasil benchmark yang akurat. Hasil perhitungan faktor multiplikasi efektif terdapat perbedaan maksimum adalah 0,014 % lebih besar dari referensi. Sedangkan untuk perhitungan faktor puncak daya, terdapat perbedaan maksimum 1,75 % dibanding harga referensi. Hasil perhitungan menunjukkan bahwa akurasi paket program PWR-FUEL dalam menghitung parameter statik benchmark reaktor PWR menunjukkan hasil yang sangat memuaskan.Kata kunci: Validasi, program PWR-FUEL, parameter statik
Energy use in informal food enterprises: A gender perspective
Authors: Matinga Margaret N. , Mohlakoana Nthabiseng , De Groot Jiska , Knox Abigail , Bressers Hans
Published: November 2018

The informal sector provides economic opportunities to the poor, and in sub-Saharan African countries it is dominated by women. Energy is a key input into the food sector enterprises. A study was carried out to review academic and non-academic literature on the use and gender impacts of modern energy in informal food enterprises. The review established that few studies have addressed energy for the informal food sector from a gender perspective. Although these few are qualitative in nature, they tend to lack in-depth analysis of gender and of the cause-and-effect linkages between modern energy use in the informal sector and the gendered goals of women and men. Moreover, a lack of understanding of gender from a relational perspective focusing on both women and men impeded conclusions on empowerment in terms of whether increased access to modern energy in the informal food sector contributes to closing the gender gap. This paper makes three key recommendations. First, scholars need to address the gaps and take a relational approach, so that studies are not just about women but also about the power relations between various groups of women and men. Secondly, policy needs to recognise that biomass is sometimes desired not just as an energy source but also for the flavour it imparts to food. Lastly, policy should be informed by the needs of informal enterprise owners and their customers, not by the general discourse in the energy sector that assumes that increased uptake of modern energy services makes positive contributions to enterprises.
L'informe ambiental en el plantejament urbanístic
Authors: Martín Abellan Irene
Published: November 2018

Aquest treball pretén explicar la lògica que ha dut el legislador català a incloure la figura de l?informe ambiental en la fase de planificació i vol donar resposta, des de l?òptica jurídica, a la pregunta a la qual senfronta el professional en l?actualitat: què és un informe ambiental, quina finalitat té, quins requisits té i quina aplicabilitat pràctica té i en què es diferencia de l?estudi d?impacte ambiental.
High-throughput miniaturized 16S rRNA amplicon library preparation v1
Authors: Minich Jeremiah , Humphrey Greg , A Salido Benitez Rodolfo , Sanders Jon , Swafford Austin , Allen Eric , Knight Rob
Published: November 2018

Next Generation Sequencing technologies have enabled many advances across biology with microbial ecology benefiting primarily through expanded sample sizes. Although the cost of running sequencing instruments has decreased substantially over time, the price of library preparation methods has largely remained unchanged. We developed a low cost, miniaturized (5 µL), high-throughput (384-sample), amplicon library preparation method with the acoustic liquid handler, Echo 550. Our method reduces costs of library preparation to $1.42 USD per sample, a 58% reduction compared to existing automated methods and a 21-fold reduction from commercial kits, without compromising sequencing success or distorting the microbial community composition analysis. The cost savings of implementing the miniaturized library preparation (going from triplicate 25 µL reactions to triplicate 5 µL reactions) are large enough to cover a MiSeq sequencing run for 768 samples, while preserving accurate microbiome measurements.
Extracted gDNA Plate Compression v1
Authors: A Salido Benitez Rodolfo
Published: November 2018

This protocol describes how to make an acoustic droplet ejection compatible plate for liquid handling on the Echo 555 using an epMotion 5075. The protocol expects (4) 96-well gDNA plates and an empty 384-well polypropylene (PP) echo destination plate. The 4 plates will be compressed in a fully interweaved layout. The following images depict this pattern.
16S Illumina Primer Plate Compression v1
Authors: A Salido Benitez Rodolfo
Published: November 2018

This protocol describes how to make an acoustic droplet ejection compatible plate for liquid handling on the Echo 555 using an epMotion 5075. The protocol expects (4) 96-well primer plates and an empty 384-well low dead volume (LDV) echo plate. The 4 plates will be compressed in a fully interweaved layout. The following images depict this pattern.
3X PCR Replicate Pooling v1
Authors: A Salido Benitez Rodolfo
Published: November 2018

The following protocol pools miniaturized PCR replicates from (2) different 384-well plates into (1) final 384-well plate using the epMotion 5075. The miniaturized PCR reaction was done in triplicate across (3) 384-well plates and the third PCR plate will be the final storage plate for PCR products.
Sub-microliter Primer and gDNA Dispense v1
Authors: A Salido Benitez Rodolfo
Published: November 2018

The following protocol describes the acoustic droplet ejection dispenses needed for a minitaturized 16S PCR reaction using the Echo 550. The protocol expects (1) 384-Well 16S Illumina Primer Plate in an echo qualified Low Dead Volume (LDV) plate, (1) 384-Well Extracted gDNA in an echo qualified Polypropylene (PP) plate, and (3) 384-Well PCR Plates in twin.tec PCR plates.
16S Illumina Primer Plate Aliquoting (working plates 5µM Forw & Rev) v1
Authors: A Salido Benitez Rodolfo
Published: November 2018

The following protocol outlines aliquoting resuspended primers, stored at 100µM in stock plates, into working primer plates at a conentration of 5µM for both forward (barcoded) and reverse primers, using the epMotion 5075. The Knigh Lab stores primer combos premixed in working primer plates.
Drosophila Egg Dechorionation v1
Authors: M Monteith Katy
Published: November 2018

This protocol describes how to make an egg dechorionator using available lab equipment before describing how to successfully use the egg dechorionator to remove the outer chorion layer from a Drosophila egg. Egg dechorionation allows cellular analysis, quantitative microinjection and the rearing of developing offspring in a gnotobiotic or axenic environment. This protocol is written with the use of dechorionated eggs for rearing fruit flies in a gnotobiotic or axenic environment in mind. Therefore sterile techniques are described. For cellular analysis and/or quantitative microinjection the protocol can be followed but with a less stringent sterile procedure where unneccesary.
Field sampling of root-associated microbes for DNA/RNA extraction v2
Authors: Angel Roey
Published: November 2018

This protocol describes a procedure for sampling plant roots in the field for future DNA and RNA extraction for microbiome analysis. The protocol is deliberately designed to be simple and requires no electronic equipment. Root samples are preserved in LifeGuard Soil Preservation Solution for protecting against nucleic acid degradation.
Intradermal coinfection of mice ears with L. major IA-2 and Staphylococcus aureus Newman strain v1
Authors: Borbon Tiffany , Parlet Corey , Clay Gwendolyn , E. Wilson Mary
Published: November 2018

Cutaneous coinfection of bacteria with Leishmania species in mice provides a model for studying the immune effects of non-sterile inoculation of Leishmania protozoa. In this protocol, we describe a method for intradermal coinfection of mice ear pinnas with Leishmania major IA-2 and a low dose of Staphylococcus aureus Newman strain. This protocol describes the methods for S. aureus and L. major preparation, mixing, mouse anesthesia and intradermal injection as well as infection monitoring and euthanasia. This protocol can be modified for the co-inoculation of other bacterial or leishmanial species in mice.
Run Prodigal using iMicrobe v2
Authors: J J. Ponsero Alise
Published: November 2018

How to run Prodigal version 2.6.3 (Hyatt et al. 2010)through theiMicrobeplaform. Prodigalis a protein-coding gene prediction software tool for bacterial and archaeal genomes.Prodigal runs smoothly on finished genomes, draft genomes, and metagenomes. Please note that Prodigal has not been tested on viruses, and that Prodigal contains no special rules or routines to handle viral genomes. More informations aboutProdigal can be found here : https://github.com/hyattpd/Prodigal/wiki