bluuebunny/crossref_metadata_2025 huggingface dataset:
Further Evidence of a Specific Psychopathology of Addiction. Differentiation from Other Psychiatric Psychopathological Dimensions (Such as Obesity)
Authors: Maremmani Angelo , Cerniglia Luca , Cimino Silvia , Bacciardi Silvia , Rovai Luca , Pallucchini Alessandro , Spera Vincenza , Perugi Giulio , Maremmani Icro
Published: August 2017

Introduction: In this study, we used a symptomatology checklist (SCL-90) to substantiate the hypothesis that Substance Use Disorder (SUD) has its own five-dimensional psychopathology. The aim of the present study was to test whether this psychopathology can be differentiated from other psychiatric psychopathological dimensions (such as obesity). Methods: The severity and frequency of each of the five dimensions were investigated, at univariate and multivariate levels, by comparing 972 Heroin Use Disorder (HUD) patients (83.5% male, mean age 30.12 ± 6.6, range: 16–59) and 106 obese individuals (50.0% male, mean age 37.59 ± 7.6, range: 24–52). The correlations between the Body Mass Index (BMI) of obese individuals with these psychopathological dimensions were also studied. Results: Obese individuals showed higher SCL-90 total scores, global severity index scores, number of items rated positively, and positive symptoms distress index scores than HUD patients. The severity of all psychopathological dimensions was significantly higher in obese individuals. Discriminant analysis showed that Panic-Anxiety and Violence-Suicide severity were more frequent in obese patients, sufficiently so to allow differentiation between HUD (lower severity) and obese individuals (greater severity). At the reclassification level, 70.8% of obese individuals in the sample were reclassified as HUD patients. Psychopathological subtypes characterized by Panic-Anxiety and Violence-Suicide typology were more frequent in obese patients and sufficiently so as to discriminate between groups. Of obese patients, 47.2% were reclassified as HUD patients. The severity of the Worthlessness-Being Trapped dimension was sufficient to predict the BMI of obese individuals. Conclusions: Our results suggest that the five-factor psychopathology found in HUD can discriminate between HUD and obese patients, but that there is an area of overlap between the forms of psychopathology found in SUD and those found in obese patients.
Enabling the Use of Sentinel-2 and LiDAR Data for Common Agriculture Policy Funds Assignment
Authors: Estrada Jesús , Sánchez Héctor , Hernanz Lorena , Checa María , Roman Dumitru
Published: August 2017

A comprehensive strategy combining remote sensing and field data can be helpful for more effective agriculture management. Satellite data are suitable for monitoring large areas over time, while LiDAR provides specific and accurate data on height and relief. Both types of data can be used for calibration and validation purposes, avoiding field visits and saving useful resources. In this paper, we propose a process for objective and automated identification of agricultural parcel features based on processing and combining Sentinel-2 data (to sense different types of irrigation patterns) and LiDAR data (to detect landscape elements). The proposed process was validated in several use cases in Spain, yielding high accuracy rates in the identification of irrigated areas and landscape elements. An important application example of the work reported in this paper is the European Union (EU) Common Agriculture Policy (CAP) funds assignment service, which would significantly benefit from a more objective and automated process for the identification of irrigated areas and landscape elements, thereby enabling the possibility for the EU to save significant amounts of money yearly.
Spatiotemporal Assessment of Littoral Waterbirds for Establishing Ecological Indicators of Mediterranean Coastal Lagoons
Authors: Farinós-Celdrán Pablo , Robledano-Aymerich Francisco , Carreño María Francisca , Martínez-López Javier
Published: August 2017

Waterbirds are vital indicators of anthropogenic influence on the ecological status of Mediterranean coastal lagoons. Our study relates temporal waterbird data to key environmental gradients at catchment scale that have a structural or functional influence on littoral waterbird assemblages at different scales. During two full-year cycles and two additional wintering seasons, the nearshore waterbird assemblages of the Mar Menor coastal lagoon (Murcia Region, SE Spain) were monitored monthly. Several biological indicator variables were related to the anthropogenic environmental gradient in the catchment area. Results showed that there was a strong dependence of waterbird assemblages on the distance to shore, emphasizing the importance of the first 100-m band, in which many species relevant to conservation converge on food resources. Well-preserved shoreline tracts therefore had a clear positive effect on community richness and diversity values, and were correlated with the occurrence of some species. These results clearly support the need for effective protection and restoration measures of such littoral habitats. Specific responses to local disturbing processes were nested within habitat and landscape preferences, supporting the value of aquatic birds as integrative ecological signals in semi-enclosed coastal systems. Moreover, waterbird-based indicators responded positively to environmental improvements both qualitatively and quantitatively.
An Internet-Based GIS Platform Providing Data for Visualization and Spatial Analysis of Urbanization in Major Asian and African Cities
Authors: Gong Hao , Simwanda Matamyo , Murayama Yuji
Published: August 2017

Rapid urbanization in developing countries has been observed to be relatively high in the last two decades, especially in the Asian and African regions. Although many researchers have made efforts to improve the understanding of the urbanization trends of various cities in Asia and Africa, the absence of platforms where local stakeholders can visualize and obtain processed urbanization data for their specific needs or analysis, still remains a gap. In this paper, we present an Internet-based GIS platform called MEGA-WEB. The Platform was developed in view of the urban planning and management challenges in developing countries of Asia and Africa due to the limited availability of data resources, effective tools, and proficiency in data analysis. MEGA-WEB provides online access, visualization, spatial analysis, and data sharing services following a mashup framework of the MEGA-WEB Geo Web Services (GWS), with the third-party map services using HTML5/JavaScript techniques. Through the integration of GIS, remote sensing, geo-modelling, and Internet GIS, several indicators for analyzing urbanization are provided in MEGA-WEB to give diverse perspectives on the urbanization of not only the physical land surface condition, but also the relationships of population, energy use, and the environment. The design, architecture, system functions, and uses of MEGA-WEB are discussed in the paper. The MEGA-WEB project is aimed at contributing to sustainable urban development in developing countries of Asia and Africa.
Comprehensive Study of Multiple Stages Progressing to Nonalcoholic Steatohepatitis with Subsequent Fibrosis in SD Rats
Authors: Wang Lulu , Wu Susu , Cai Minxuan , Ma Ji , Li Shengcun , Li Maoru , Xu Yan , Wei Lixin , Shang Jing
Published: August 2017

Because of the absence of the time course of histological nonalcoholic fatty hepatitis with subsequent fibrotic progression, the effective approaches available for controlling the onset and progression of non-alcoholic steatohepatitis (NASH) remain limited. Therefore, we detected the serum and liver tissue related lipid metabolism disorder, liver pathology and relative molecular makers alteration dynamically in a high fat-sucrose diet during different time points. High fat-sucrose diet significantly increased the serum lipid level on day 10. The excess lipid accumulation in liver was referred to as simple steatosis after the feeding of a high fat-sucrose diet for 20 days. The high fat-sucrose diet induced a hepatic inflammation response on day 30. Similarly, hepatic fibrosis was also initiated on day 30 and gradually formed from the 30th to the 50th day. Oxidative stress may be related with the process from NASH to liver fibrosis. Insulin resistance was involved in the progression from hepatic steatosis to NASH with hepatic fibrosis from the 20th to the 50th day. In conclusion, we established a high fat-sucrose diet induced nonalcoholic fatty hepatitis with liver fibrosis rat model, which presented the time course of histological nonalcoholic steatohepatitis and the initiation and progression change of characteristic molecular makers in the process from steatosis to hepatic fibrosis.
A Review of Anti-Angiogenic Targets for Monoclonal Antibody Cancer Therapy
Authors: Kong Deok-Hoon , Kim Mi , Jang Ji , Na Hee-Jun , Lee Sukmook
Published: August 2017

Tumor angiogenesis is a key event that governs tumor progression and metastasis. It is controlled by the complicated and coordinated actions of pro-angiogenic factors and their receptors that become upregulated during tumorigenesis. Over the past several decades, vascular endothelial growth factor (VEGF) signaling has been identified as a central axis in tumor angiogenesis. The remarkable advent of recombinant antibody technology has led to the development of bevacizumab, a humanized antibody that targets VEGF and is a leading clinical therapy to suppress tumor angiogenesis. However, despite the clinical efficacy of bevacizumab, its significant side effects and drug resistance have raised concerns necessitating the identification of novel drug targets and development of novel therapeutics to combat tumor angiogenesis. This review will highlight the role and relevance of VEGF and other potential therapeutic targets and their receptors in angiogenesis. Simultaneously, we will also cover the current status of monoclonal antibodies being developed to target these candidates for cancer therapy.
Dysfunctional Natural Killer Cells in the Aftermath of Cancer Surgery
Authors: Angka Leonard , Khan Sarwat , Kilgour Marisa , Xu Rebecca , Kennedy Michael , Auer Rebecca
Published: August 2017

The physiological changes that occur immediately following cancer surgeries initiate a chain of events that ultimately result in a short pro-, followed by a prolonged anti-, inflammatory period. Natural Killer (NK) cells are severely affected during this period in the recovering cancer patient. NK cells play a crucial role in anti-tumour immunity because of their innate ability to differentiate between malignant versus normal cells. Therefore, an opportunity arises in the aftermath of cancer surgery for residual cancer cells, including distant metastases, to gain a foothold in the absence of NK cell surveillance. Here, we describe the post-operative environment and how the release of sympathetic stress-related factors (e.g., cortisol, prostaglandins, catecholamines), anti-inflammatory cytokines (e.g., IL-6, TGF-β), and myeloid derived suppressor cells, mediate NK cell dysfunction. A snapshot of current and recently completed clinical trials specifically addressing NK cell dysfunction post-surgery is also discussed. In collecting and summarizing results from these different aspects of the surgical stress response, a comprehensive view of the NK cell suppressive effects of surgery is presented. Peri-operative therapies to mitigate NK cell suppression in the post-operative period could improve curative outcomes following cancer surgery.
The Role of Neurogenic Inflammation in Blood-Brain Barrier Disruption and Development of Cerebral Oedema Following Acute Central Nervous System (CNS) Injury
Authors: Sorby-Adams Annabel , Marcoionni Amanda , Dempsey Eden , Woenig Joshua , Turner Renée
Published: August 2017

Acute central nervous system (CNS) injury, encompassing traumatic brain injury (TBI) and stroke, accounts for a significant burden of morbidity and mortality worldwide, largely attributable to the development of cerebral oedema and elevated intracranial pressure (ICP). Despite this, clinical treatments are limited and new therapies are urgently required to improve patient outcomes and survival. Originally characterised in peripheral tissues, such as the skin and lungs as a neurally-elicited inflammatory process that contributes to increased microvascular permeability and tissue swelling, neurogenic inflammation has now been described in acute injury to the brain where it may play a key role in the secondary injury cascades that evolve following both TBI and stroke. In particular, release of the neuropeptides substance P (SP) and calcitonin gene-related peptide (CGRP) appear to be critically involved. In particular, increased SP expression is observed in perivascular tissue following acute CNS injury, with the magnitude of SP release being related to both the frequency and degree of the insult. SP release is associated with profound blood-brain barrier disruption and the subsequent development of vasogenic oedema, as well as neuronal injury and poor functional outcomes. Inhibition of SP through use of a neurokinin 1 (NK1) antagonist is highly beneficial following both TBI and ischaemic stroke in pre-clinical models. The role of CGRP is more unclear, especially with respect to TBI, with both elevations and reductions in CGRP levels reported following trauma. However, a beneficial role has been delineated in stroke, given its potent vasodilatory effects. Thus, modulating neuropeptides represents a novel therapeutic target in the treatment of cerebral oedema following acute CNS injury.
Photoconvertible Fluorescent Proteins and the Role of Dynamics in Protein Evolution
Authors: Wachter Rebekka
Published: August 2017

Photoconvertible fluorescent proteins (pcFPs) constitute a large group of fluorescent proteins related to green fluorescent protein (GFP) that, when exposed to blue light, bear the capability of irreversibly switching their emission color from green to red. Not surprisingly, this fascinating class of FPs has found numerous applications, in particular for the visualization of biological processes. A detailed understanding of the photoconversion mechanism appears indispensable in the design of improved variants for applications such as super-resolution imaging. In this article, recent work is reviewed that involves using pcFPs as a model system for studying protein dynamics. Evidence has been provided that the evolution of pcFPs from a green ancestor involved the natural selection for altered dynamical features of the beta-barrel fold. It appears that photoconversion may be the outcome of a long-range positional shift of a fold-anchoring region. A relatively stiff, rigid element appears to have migrated away from the chromophore-bearing section to the opposite edge of the barrel, thereby endowing pcFPs with increased active site flexibility while keeping the fold intact. In this way, the stage was set for the coupling of light absorption with subsequent chemical transformations. The emerging mechanistic model suggests that highly specific dynamic motions are linked to key chemical steps, preparing the system for a concerted deprotonation and β-elimination reaction that enlarges the chromophore’s π-conjugation to generate red color.
High-Throughput Screening Assay for Laccase Engineering toward Lignosulfonate Valorization
Authors: Rodríguez-Escribano David , De Salas Felipe , Pardo Isabel , Camarero Susana
Published: August 2017

Lignin valorization is a pending issue for the integrated conversion of lignocellulose in consumer goods. Lignosulfonates (LS) are the main technical lignins commercialized today. However, their molecular weight should be enlarged to meet application requirements as additives or dispersing agents. Oxidation of lignosulfonates with fungal oxidoreductases, such as laccases, can increase the molecular weight of lignosulfonates by the cross-linking of lignin phenols. To advance in this direction, we describe here the development of a high-throughput screening (HTS) assay for the directed evolution of laccases, with lignosulfonate as substrate and the Folin–Ciocalteau reagent (FCR), to detect the decrease in phenolic content produced upon polymerization of lignosulfonate by the enzyme. Once the reaction conditions were adjusted to the 96-well-plate format, the enzyme for validating the assay was selected from a battery of high-redox-potential laccase variants functionally expressed in S. cerevisiae (the preferred host for the directed evolution of fungal oxidoreductases). The colorimetric response (absorbance at 760 nm) correlated with laccase activity secreted by the yeast. The HTS assay was reproducible (coefficient of variation (CV) = 15%) and sensitive enough to detect subtle differences in activity among yeast clones expressing a laccase mutant library obtained by error-prone PCR (epPCR). The method is therefore feasible for screening thousands of clones during the precise engineering of laccases toward valorization of lignosulfonates.
Rho-Kinase Blockade Attenuates Podocyte Apoptosis by Inhibiting the Notch Signaling Pathway in Diabetic Nephropathy
Authors: Matoba Keiichiro , Kawanami Daiji , Nagai Yosuke , Takeda Yusuke , Akamine Tomoyo , Ishizawa Sho , Kanazawa Yasushi , Yokota Tamotsu , Utsunomiya Kazunori
Published: August 2017

Podocyte apoptosis is a key process in the onset of diabetic nephropathy. A significant body of evidence shows that the Notch signaling pathway plays a central role in this process. We found that Rho-kinase mediates transforming growth factor β (TGF-β)-induced Notch ligand Jag1 expression. Importantly, TGF-β-mediated podocyte apoptosis was attenuated by Rho-kinase inhibition. Mechanistically, Rho-kinase regulated Jag1 induction via the extracellular signal-regulated kinase (ERK) 1/2 and c-Jun N-terminal kinase (JNK) but not Smad pathways. Consistently, the Rho-kinase inhibitor fasudil prevented albuminuria and the urinary excretion of nephrin in db/db mice and reduced the prevalence of podocyte apoptosis and Jag1 expression. Finally, the expression of Jag1 and apoptosis markers such as Bax and cyclin-dependent kinase inhibitor 1A (CDKN1A) was decreased in podocytes derived from db/db mice treated with fasudil. The present study provides evidence that Rho-kinase plays a key role in podocyte apoptosis. Rho-kinase is an attractive therapeutic target for diabetic nephropathy.
Immune-Response Patterns and Next Generation Sequencing Diagnostics for the Detection of Mycoses in Patients with Septic Shock—Results of a Combined Clinical and Experimental Investigation
Authors: Decker Sebastian , Sigl Annette , Grumaz Christian , Stevens Philip , Vainshtein Yevhen , Zimmermann Stefan , Weigand Markus , Hofer Stefan , Sohn Kai , Brenner Thorsten
Published: August 2017

Fungi are of increasing importance in sepsis. However, culture-based diagnostic procedures are associated with relevant weaknesses. Therefore, culture- and next-generation sequencing (NGS)-based fungal findings as well as corresponding plasma levels of β-d-glucan, interferon gamma (INF-γ), tumor necrosis factor alpha (TNF-α), interleukin (IL)-2, -4, -6, -10, -17A, and mid-regional proadrenomedullin (MR-proADM) were evaluated in 50 septic patients at six consecutive time points within 28 days after sepsis onset. Furthermore, immune-response patterns during infections with Candida spp. were studied in a reconstituted human epithelium model. In total, 22% (n = 11) of patients suffered from a fungal infection. An NGS-based diagnostic approach appeared to be suitable for the identification of fungal pathogens in patients suffering from fungemia as well as in patients with negative blood cultures. Moreover, MR-proADM and IL-17A in plasma proved suitable for the identification of patients with a fungal infection. Using RNA-seq., adrenomedullin (ADM) was shown to be a target gene which is upregulated early after an epithelial infection with Candida spp. In summary, an NGS-based diagnostic approach was able to close the diagnostic gap of routinely used culture-based diagnostic procedures, which can be further facilitated by plasmatic measurements of MR-proADM and IL-17A. In addition, ADM was identified as an early target gene in response to epithelial infections with Candida spp.
Determination of Sphingosine-1-Phosphate in Human Plasma Using Liquid Chromatography Coupled with Q-Tof Mass Spectrometry
Authors: Egom Emmanuel , Fitzgerald Ross , Canning Rebecca , Pharithi Rebabonye B. , Murphy Colin , Maher Vincent
Published: August 2017

Evidence suggests that high-density lipoprotein (HDL) components distinct from cholesterol, such as sphingosine-1-phosphate (S1P), may account for the anti-atherothrombotic effects attributed to this lipoprotein. The current method for the determination of plasma levels of S1P as well as levels associated with HDL particles is still cumbersome an assay method to be worldwide practical. Recently, a simplified protocol based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the sensitive and specific quantification of plasma levels of S1P with good accuracy has been reported. This work utilized a triple quadrupole (QqQ)-based LC-MS/MS system. Here we adapt that method for the determination of plasma levels of S1P using a quadrupole time of flight (Q-Tof) based LC-MS system. Calibration curves were linear in the range of 0.05 to 2 µM. The lower limit of quantification (LOQ) was 0.05 µM. The concentration of S1P in human plasma was determined to be 1 ± 0.09 µM (n = 6). The average accuracy over the stated range of the method was found to be 100 ± 5.9% with precision at the LOQ better than 10% when predicting the calibration standards. The concentration of plasma S1P in the prepared samples was stable for 24 h at room temperature. We have demonstrated the quantification of plasma S1P using Q-Tof based LC-MS with very good sensitivity, accuracy, and precision that can used for future studies in this field.
EpCAM Expression in Lymph Node Metastases of Urothelial Cell Carcinoma of the Bladder: A Pilot Study
Authors: Van der Fels Christa , Rosati Stefano , De Jong Igle
Published: August 2017

In this retrospective pilot study, the feasibility of the epithelial cell adhesion molecule (EpCAM) as an imaging target for lymph node (LN) metastatic disease of urothelial cell carcinoma (UCC) of the bladder was investigated. LN metastases and LNs without metastases of patients who underwent pelvic lymph node dissection because of muscle invasive bladder cancer (MIBC) were used. Primary tumors of the same patients were used from cystectomy specimen, transurethral resections, and biopsies. A pathologist, blinded to clinical data, scored EpCAM immunoreactivity. This method determines a total immunostaining score, which is the product of a proportion score and an intensity score. EpCAM expression was observed in 19/20 (95%) LNs with UCC metastases and in 11/12 (92%) of the primary tumors. EpCAM expression was absent in 14/14 (100%) LNs without metastases. Median EpCAM expression (TIS) in LN metastases was 5 (IQR 2.0–8.0) and in the primary tumors 6 (IQR 2.3–11.0). Based on the absence of staining in LNs without metastases, EpCAM show high tumor distinctiveness. EpCAM seems to be a feasible imaging target in LN metastases of UCC of the bladder. Pre- and perioperative visualization of these metastases will improve disease staging and improve the complete resection of LN metastases in MIBC.
Platelet Aggregometry Testing: Molecular Mechanisms, Techniques and Clinical Implications
Authors: Koltai Katalin , Kesmarky Gabor , Feher Gergely , Tibold Antal , Toth Kalman
Published: August 2017

Platelets play a fundamental role in normal hemostasis, while their inherited or acquired dysfunctions are involved in a variety of bleeding disorders or thrombotic events. Several laboratory methodologies or point-of-care testing methods are currently available for clinical and experimental settings. These methods describe different aspects of platelet function based on platelet aggregation, platelet adhesion, the viscoelastic properties during clot formation, the evaluation of thromboxane metabolism or certain flow cytometry techniques. Platelet aggregometry is applied in different clinical settings as monitoring response to antiplatelet therapies, the assessment of perioperative bleeding risk, the diagnosis of inherited bleeding disorders or in transfusion medicine. The rationale for platelet function-driven antiplatelet therapy was based on the result of several studies on patients undergoing percutaneous coronary intervention (PCI), where an association between high platelet reactivity despite P2Y12 inhibition and ischemic events as stent thrombosis or cardiovascular death was found. However, recent large scale randomized, controlled trials have consistently failed to demonstrate a benefit of personalised antiplatelet therapy based on platelet function testing.
NPFFR2 Activates the HPA Axis and Induces Anxiogenic Effects in Rodents
Authors: Lin Ya-Tin , Yu Yu-Lian , Hong Wei-Chen , Yeh Ting-Shiuan , Chen Ting-Chun , Chen Jin-Chung
Published: August 2017

Neuropeptide FF (NPFF) belongs to the RFamide family and is known as a morphine-modulating peptide. NPFF regulates various hypothalamic functions through two receptors, NPFFR1 and NPFFR2. The hypothalamic-pituitary-adrenal (HPA) axis participates in physiological stress response by increasing circulating glucocorticoid levels and modulating emotional responses. Other RFamide peptides, including neuropeptide AF, neuropeptide SF and RFamide related peptide also target NPFFR1 or NPFFR2, and have been reported to activate the HPA axis and induce anxiety- or depression-like behaviors. However, little is known about the action of NPFF on HPA axis activity and anxiety-like behaviors, and the role of the individual receptors remains unclear. In this study, NPFFR2 agonists were used to examine the role of NPFFR2 in activating the HPA axis in rodents. Administration of NPFFR2 agonists, dNPA (intracerebroventricular, ICV) and AC-263093 (intraperitoneal, IP), time-dependently (in rats) and dose-dependently (in mice) increased serum corticosteroid levels and the effects were counteracted by the NPFF receptor antagonist, RF9 (ICV), as well as corticotropin-releasing factor (CRF) antagonist, α-helical CRF(9-41) (intravenous, IV). Treatment with NPFFR2 agonist (AC-263093, IP) increased c-Fos protein expression in the hypothalamic paraventricular nucleus and induced an anxiogenic effect, which was evaluated in mice using an elevated plus maze. These findings reveal, for the first time, that the direct action of hypothalamic NPFFR2 stimulates the HPA axis and triggers anxiety-like behaviors.
Multidimensional Data Exploration by Explicitly Controlled Animation
Authors: Kruiger Johannes , Hassoumi Almoctar , Schulz Hans-Jörg , Telea AlexandruC , Hurter Christophe
Published: August 2017

Understanding large multidimensional datasets is one of the most challenging problems in visual data exploration. One key challenge that increases the size of the exploration space is the number of views that one can generate from a single dataset, based on the use of multiple parameter values and exploration paths. Often, no such single view contains all needed insights. The question thus arises of how we can efficiently combine insights from multiple views of a dataset. We propose a set of techniques that considerably reduce the exploration effort for such situations, based on the explicit depiction of the view space, using a small multiple metaphor. We leverage this view space by offering interactive techniques that enable users to explicitly create, visualize, and follow their exploration path. This way, partial insights obtained from each view can be efficiently and effectively combined. We demonstrate our approach by applications using real-world datasets from air traffic control, software maintenance, and machine learning.
Control of Spin-Crossover Phenomena in One-Dimensional Triazole-Coordinated Iron(II) Complexes by Means of Functional Counter Ions
Authors: Sugahara Akira , Kamebuchi Hajime , Okazawa Atsushi , Enomoto Masaya , Kojima Norimichi
Published: August 2017

The spin-crossover (SCO) phenomenon between a high-spin and a low-spin state has attracted much attention in the field of materials science. Among the various kinds of SCO complexes, the triazole-bridged iron(II) polymeric chain system, [Fe(II)(R-trz)3]X2·xH2O (where trz is triazole and X is the anion), exhibiting the SCO phenomenon with thermal hysteresis around room temperature, has been extensively studied from the viewpoint of molecular memory and molecular devices. In connection with this system, we have controlled the SCO phenomenon according to the characteristic properties of counter ions. In the case of X being CnH2n+1SO3−, the spin transition temperature (T1/2) increases with increasing the length (n) of the alkyl chain of the counter ion and saturates above n = 5, which is attributed to the increase in the intermolecular interaction of the alkyl chains of CnH2n+1SO3−, called the fastener effect. The hysteresis width of T1/2 decreases with increasing n, showing the even-odd, also known as parity, effect. In the cases where X is toluenesulfonate (tos: CH3C6H4SO3−) and aminobenzenesulfonate (abs: NH2C6H4SO3−), T1/2 and its hysteresis width vary drastically with the structural isomerism (ortho-, metha-, and para-substitution) of counter ions, which implies the possibility of photoinduced spin transition by means of the photoisomerization of counter ions. From this strategy, we have synthesized [Fe(II)(NH2-trz)3](SP150)2·2H2O (SP150 = N-alkylsulfonated spiropyran) and investigated the SCO phenomenon. Moreover, we have developed [Fe(II)(R-trz)3]@Nafion films exhibiting spin transition around room temperature, where the Nafion membrane behaves as a counter anion as well as a transparent substrate, and investigated the photogenerated high-spin state below 35 K. The lifetime of the photogenerated high-spin state strongly depends on the intensity of irradiated light.
Minimal Pruning and Reduced Plant Protection Promote Predatory Mites in Grapevine
Authors: Pennington Theresa , Kraus Christian , Alakina Ekatarina , Entling Martin , Hoffmann Christoph
Published: August 2017

Improving natural pest control by promoting high densities of predatory mites (Acari: Phytoseiidae) is an effective way to prevent damage by pest mites (e.g., Eriophyidae, Tetranychidae) and other arthropod taxa that can cause serious damage to vineyards. Here, we investigate the influence of innovative management on predatory mite densities. We compare (i) full versus reduced fungicide applications and (ii) minimal pruning versus a traditional trellis pruning system in four fungus-resistant grapevine varieties. As predatory mites also feed on fungus mycelium, we assessed fungal infection of grapevine leaves in the experimental vineyard. Predatory mites were significantly more abundant in both minimal pruning and under reduced plant protection. Increases in predatory mites appeared to be independent of fungal infection, suggesting mostly direct effects of reduced fungicides and minimal pruning. In contrast to predatory mites, pest mites did not increase under innovative management. Thus, conditions for natural pest control are improved in fungus-resistant grapevines and under minimal pruning, which adds to other advantages such as environmental safety and reduced production cost.
A Novel Nested Reconfigurable Approach for a Glass Façade Cleaning Robot
Authors: Nansai Shunsuke , Elara Mohan , Tun Thein , Veerajagadheswar Prabakaran , Pathmakumar Thejus
Published: August 2017

The façade cleaning of high rise buildings is one of the hazardous tasks that is performed by human operators. Even after a significant advancement in construction technologies, several newfangled skyscrapers are still using the manual method for cleaning the glass panels. This research is aimed at the development of a glass façade cleaning robot, capable of adapting to any kind of building architecture. A robotic system capable of cleaning vertical glass surfaces demands a transformable morphology. A self-reconfigurable robot is one of the potential solutions to realize high degrees of adaptability. Following the design principles we derived, we propose a nested reconfigurable design approach for glass façade cleaning and develope a system of robot modules that performs glass façade cleaning. Throughout this research article, we discuss the brief concept and scheme of nested reconfigurable design principle and the hardware-software challenges associated with it. This article also discusses the capability to maximize the flexibility and modularity of the robot by using intra- and inter-reconfigurations. The effectiveness of the designed system is verified by experimental means.
Use of FGF-21 as a Biomarker of Mitochondrial Disease in Clinical Practice
Authors: Morovat Alireza , Weerasinghe Gayani , Nesbitt Victoria , Hofer Monika , Agnew Thomas , Quaghebeur Geralrine , Sergeant Kate , Fratter Carl , Guha Nishan , Mirzazadeh Mehdi , Poulton Joanna
Published: August 2017

Recent work has suggested that fibroblast growth factor-21 (FGF-21) is a useful biomarker of mitochondrial disease (MD). We routinely measured FGF-21 levels on patients who were investigated at our centre for MD and evaluated its diagnostic performance based on detailed genetic and other laboratory findings. Patients’ FGF-21 results were assessed by the use of age-adjusted z-scores based on normalised FGF-21 values from a healthy population. One hundred and fifty five patients were investigated. One hundred and four of these patients had molecular evidence for MD, 27 were deemed to have disorders other than MD (non-MD), and 24 had possible MD. Patients with defects in mitochondrial DNA (mtDNA) maintenance (n = 32) and mtDNA rearrangements (n = 17) had the highest median FGF-21 among the MD group. Other MD patients harbouring mtDNA point mutations (n = 40) or mutations in other autosomal genes (n = 7) and those with partially characterised MD had lower FGF-21 levels. The area under the receiver operating characteristic curve for distinguishing MD from non-MD patients was 0.69. No correlation between FGF-21 and creatinine, creatine kinase, or cardio-skeletal myopathy score was found. FGF-21 was significantly associated with plasma lactate and ocular myopathy. Although FGF-21 was found to have a low sensitivity for detecting MD, at a z-score of 2.8, its specificity was above 90%. We suggest that a high serum concentration of FGF-21 would be clinically useful in MD, especially in adult patients with chronic progressive external ophthalmoplegia, and may enable bypassing muscle biopsy and directly opting for genetic analysis. Availability of its assay has thus modified our diagnostic pathway.
A Comparative Analysis of the Reinforcing Efficiency of Silsesquioxane Nanoparticles versus Apatite Nanoparticles in Chitosan Biocomposite Fibres
Authors: Wang Kean , Pasbakhsh Pooria , De Silva Rangika , Goh Kheng
Published: August 2017

A comparative analysis of the effects of polyhedral oligomeric silsesquioxane (POSS) and hydroxyapatite (HA) for reinforcing chitosan (CS) is given here. Wet-spun CS nanocomposite fibres, blended with HA or POSS nanoparticles, at varying concentrations ranging from 1 to 9% (w/w) were stretched until rupture to determine the mechanical properties related to the elasticity (yield strength and strain, stiffness, resilience energy) and fracture (fracture strength strain and toughness) of the composite. Two-factor analysis of variance of the data concluded that only the fracture-related properties were sensitive to interaction effects between the particle type and concentration. When particle type is considered, the stiffness and yield strength of CS/POSS fibres are higher than CS/HA fibres—the converse holds for yield strain, extensibility and fracture toughness. With regards to sensitivity to particle concentration, stiffness and yield strength reveal trending increase to a peak value (the optimal particle concentration associated with the critical aggregation) and trending decrease thereafter, with increasing particle concentration. Although fracture strength, strain at fracture and fracture toughness are also sensitive to particle concentration, no apparent trending increase/decrease is sustained over the particle concentration range investigated here. This simple study provides further understanding into the mechanics of particle-reinforced composites—the insights derived here concerning the optimized mechanical properties of chitosan composite fibre may be further developed to permit us to tune the mechanical properties to suit the biomedical engineering application.
Effect of Bottom Friction, Wind Drag Coefficient, and Meteorological Forcing in Hindcast of Hurricane Rita Storm Surge Using SWAN + ADCIRC Model
Authors: Akbar Muhammad , Kanjanda Simbarashe , Musinguzi Abram
Published: August 2017

An evaluation of the effect of bottom friction, wind drag coefficient, and meteorological forcing is conducted using a tightly coupled wave and circulation model, SWAN + ADCIRC (i.e., Simulating WAves Nearshore + ADvanced CIRCulation), to hindcast the storm surge of Hurricane Rita (2005). Wind drag coefficient formulations of Powell, Zijlema, and Peng & Li are used to calculate wind stresses. Bottom friction and wind drag coefficients are systematically increased and decreased to quantify their impacts on the hindcast. Different meteorological forcing options are applied to study the effect of wind fields on storm surge development and propagation. Simulated water levels are compared with observed data collected from about 150 locations. It is evident that a lower bottom friction causes higher and faster surge propagation, and earlier arrival of inundation peak at locations far from the land fall. Drag coefficients of Powell, with or without a cap of 0.002, and Zijlema produce similar results, while that of Peng & Li slightly overpredicted the surge. Wind fields may cause overprediction or underprediction of the surge, depending on the choice of the wind model. A good agreement is found between Zijlema’s findings and this study; that simultaneously decreasing or increasing both bottom friction and wind drag essentially provides the same hindcast results.
Enhanced IoT-Based End-To-End Emergency and Disaster Relief System
Authors: Ben Arbia Dhafer , Alam Muhammad , Kadri Abdullah , Ben Hamida Elyes , Attia Rabah
Published: August 2017

In this paper, we present a new enhancement for an emergency and disaster relief system called Critical and Rescue Operations using Wearable Wireless sensors networks (CROW 2 ). We address the reliability challenges in setting up a wireless autonomous communication system in order to offload data from the disaster area (rescuers, trapped victims, civilians, media, etc.) back to a command center. The proposed system connects deployed rescuers to extended networks and the Internet. CROW 2 is an end-to-end system that runs the recently-proposed Optimized Routing Approach for Critical and Emergency Networks (ORACE-Net) routing protocol. The system integrates heterogeneous wireless devices (Raspberry Pi, smart phones, sensors) and various communicating technologies (WiFi IEEE 802.11n, Bluetooth IEEE 802.15.1) to enable end-to-end network connectivity, which is monitored by a cloud Internet-of-Things platform. First, we present the CROW 2 generic system architecture, which is adaptable to various technologies integration at different levels (i.e., on-body, body-to-body, off-body). Second, we implement the ORACE-Net protocol on heterogeneous devices including Android-based smart phones and Linux-based Raspberry Pi devices. These devices act as on-body coordinators to collect information from on-body sensors. The collected data is then pushed to the command center thanks to multi-hop device-to-device communication. Third, the overall CROW 2 system performance is evaluated according to relevant metrics including end-to-end link quality estimation, throughput and end-to-end delay. As a proof-of-concept, we validate the system architecture through deployment and extracted experimental results. Finally, we highlight motion detection and links’ unavailability prevention based on the recorded data where the main factors (i.e., interference and noise) that affect the performance are analyzed.
Human Trafficking and Study Abroad
Authors: Parker Taylor S.
Published: August 2017

There are many risks that students face while abroad; from tragic accidents, illness, and disease to becoming victims of violent crimes. Human trafficking is an international threat facing everyone. While victims of human trafficking come from all walks of life, in particular, individuals belonging to vulnerable populations are often targeted for this method of exploitation. Cultural competency, language barriers, and ignorance as to resources are all factors which contribute to the increased vulnerability of students studying abroad. An institution providing opportunities for international study should develop an effective approach to mitigate the risk of human trafficking through programs designed to enable students to protect themselves and others effectively. This paper comments on best practices for risk management, and explores different avenues and relevant law for increased transparency in study abroad risk.
Film Thickness and Friction Relationship in Grease Lubricated Rough Contacts
Authors: Gonçalves David , Vieira António , Carneiro António , Campos Armando , Seabra Jorge
Published: August 2017

The relationship between the film generation and the coefficient of friction in grease lubricated contacts was investigated. Ball-on-disc tests were performed under different operating conditions: entrainment speed, lubricant temperature and surface roughness. The tests were performed with fully formulated greases and their base oils. The greases were formulated with different thickener types and also different base oils natures and viscosities. Film thickness measurements were performed in ball-on-glass disc tests, and Stribeck curves were measured in ball-on-steel disc tests with discs of different roughness. The role of the thickener and the base oil nature/viscosity on the film thickness and coefficient of friction was addressed and the greases’ performance was compared based on their formulation.
Production of Synthetic Phosphoanhydrite and Its Use as a Binder in Self-Leveling Underlayments (SLU)
Authors: Schaefer Cecília , Cheriaf Malik , Rocha Janaíde
Published: August 2017

An experimental study was conducted to investigate the potential use of phosphogypsum (PG) to produce self-leveling underlayments. The study was designed in two stages. Initially a phosphoanhydrite (PA) was produced by heating phosphogypsum at temperatures of 350 °C, 450 °C, 550 °C, and 650 °C. Two periods of heating were applied (2 and 4 h). The formation of anhydrite was determined by thermogravimetric analysis (DTA-TG) and confirmed by X-ray diffraction (XRD). The results show that anhydrite II was obtained at temperatures above 450 °C, and at higher calcination temperatures the PA solubility was lower. In the second stage of this research, the PA was used in self-leveling underlayments as the main binder in the ternary system comprised of calcium sulfate, calcium aluminate cement, and Portland cement. Self-leveling mortar screeds produced using PA (550 °C/4 h) and PA (650 °C/4 h) showed the best performance in terms of mechanical strength and no degradation was observed after immersion and immersion-drying tests. The formation of ettringite, identified by scanning electron microscopy (SEM), may have contributed to these results. Morphological changes were studied using the scanning electron microscopy (SEM) technique.
A Novel Approach to Eliminate the Effect of External Stress on Interdiffusivity Measurement
Authors: Chen Weimin , Li Qin , Zhang Lijun
Published: August 2017

In this paper, the interdiffusivities in fcc Co–Ni alloys at 1373 K due to different types of diffusion couple experiments were firstly re-calculated via the unified Wagner method based on the measured composition profiles. Their maximum difference due to different approaches for diffusion couple preparation was found to be larger than one order of magnitude. Then, a comprehensive analysis on the effect of different preparation methods was performed. After that, a two-step diffusion couple technique in combination with the pragmatic numerical inverse method was proposed to determine the accurate interdiffusivities by eliminating the effect of external stress. Such a novel approach was successfully applied in the binary fcc Co–Ni alloys for demonstration purposes. Moreover, it is anticipated that such novel approach can be utilized as the standard method for accurate interdiffusivity measurement, and the resultant accurate interdiffusivities in different alloys may serve as a benchmark for the later experimental and theoretical studies.
Multiphysics Simulation of Low-Amplitude Acoustic Wave Detection by Piezoelectric Wafer Active Sensors Validated by In-Situ AE-Fatigue Experiment
Authors: Bhuiyan Md Yeasin , Giurgiutiu Victor
Published: August 2017

Piezoelectric wafer active sensors (PWAS) are commonly used for detecting Lamb waves for structural health monitoring application. However, in most applications of active sensing, the signals are of high-amplitude and easy to detect. In this article, we have shown a new avenue of using the PWAS transducer for detecting the low-amplitude fatigue-crack related acoustic emission (AE) signals. Multiphysics finite element (FE) simulations were performed with two PWAS transducers bonded to the structure. Various configurations of the sensors were studied by using the simulations. One PWAS was placed near to the fatigue-crack and the other one was placed at a certain distance from the crack. The simulated AE event was generated at the crack tip. The simulation results showed that both PWAS transducers were capable of sensing the AE signals. To validate the multiphysics simulation results, an in-situ AE-fatigue experiment was performed. Two PWAS transducers were bonded to the thin aerospace test coupon. The fatigue crack was generated in the test coupon which had produced low-amplitude acoustic waves. The low-amplitude fatigue-crack related AE signals were successfully captured by the PWAS transducers. The distance effect on the captured AE signals was also studied. It has been shown that some high-frequency contents of the AE signal have developed as they travel away from the crack.
Measurement Techniques of the Magneto-Electric Coupling in Multiferroics
Authors: Vopson M. M. , Fetisov Y. K. , Caruntu G. , Srinivasan G.
Published: August 2017

The current surge of interest in multiferroic materials demands specialized measurement techniques to support multiferroics research. In this review article we detail well-established measurement techniques of the magneto-electric coupling coefficient in multiferroic materials, together with newly proposed ones. This work is intended to serve as a reference document for anyone willing to develop experimental measurement techniques of multiferroic materials.
Mussel-Inspired Dopamine and Carbon Nanotube Leading to a Biocompatible Self-Rolling Conductive Hydrogel Film
Authors: Jiang Junzi , Huang Yong , Wang Yitian , Xu Hui , Xing Malcolm , Zhong Wen
Published: August 2017

We report a novel self-rolling, conductive, and biocompatible multiwall carbon nanotube (MWCNT)-dopamine-polyethylene glycol (PEG) hydrogel film. The gel can self-fold into a thin tube when it is transferred from a glass slide to an aqueous environment, regardless of the concentrations of the MWCNT. The film presents a highly organized pattern, which results from the self-assembly of hydrophilic dopamine and hydrophobic carbon nanotubes. By exploring the biomedical potential, we found that MWCNT-included rolled film is nontoxic and can promote cell growth. For further functional verification by qPCR (quantitative polymerase chain reaction), bone marrow derived mesenchymal cells present higher levels of osteogenic differentiations in response to a higher concentration of CNTs. The results suggest that the self-rolling, conductive CNT-dopamine-PEG hydrogel could have multiple potentials, including biomedical usage and as a conductive biosensor.