bluuebunny/crossref_metadata_2025 huggingface dataset:
3D Face Recognition: Two Decades of Progress and Prospects
Authors: Guo Yulan , Wang Hanyun , Wang Longguang , Lei Yinjie , Liu Li , Bennamoun Mohammed
Published: August 2023

Three-dimensional (3D) face recognition has been extensively investigated in the last two decades due to its wide range of applications in many areas, such as security and forensics. Numerous methods have been proposed to deal with the challenges faced by 3D face recognition, such as facial expressions, pose variations, and occlusions. These methods have achieved superior performances on several small-scale datasets, including FRGC v2.0, Bosphorus, BU-3DFE, and Gavab. However, deep learning–based 3D face recognition methods are still in their infancy due to the lack of large-scale 3D face datasets. To stimulate future research in this area, we present a comprehensive review of the progress achieved by both traditional and deep learning–based 3D face recognition methods in the last two decades. Comparative results on several publicly available datasets under different challenges of facial expressions, pose variations, and occlusions are also presented.
Muscle mitochondrial transplantation can rescue and maintain cellular homeostasis
Authors: Bhattacharya Debasmita , Slavin Mikhaela B. , Hood David A.
Published: August 2023

In our study, we have shown the potential of mouse skeletal muscle intermyofibrillar mitochondria to be transplanted in myoblasts and human fibroblast cells having mitochondrial DNA mutations. This resulted in an augmentation of mitochondrial dynamics and enhancement of bioenergetic profile in the host cells. Our findings suggest that mitochondria from donor skeletal muscle can be integrated into both healthy and functionally compromised host cells leading to mitochondrial structural refinement and respiratory boost.
Ketone ester administration improves glycemia in obese mice
Authors: Tabatabaei Dakhili Seyed Amirhossein , Yang Kunyan , Locatelli Cassandra A. A. , Saed Christina T. , Greenwell Amanda A. , Chan Jordan S. F. , Chahade Jadin J. , Scharff Jared , Al-Imarah Shahad , Eaton Farah , Crawford Peter A. , Gopal Keshav , Mulvihill Erin E. , Ussher John R.
Published: August 2023

It has been demonstrated that increased skeletal muscle ketone metabolism contributes to obesity-related hyperglycemia. Since increases in ketone supply are key determinants of organ ketone oxidation rates, we determined whether acute elevations in circulating ketones following administration of an oral ketone ester may worsen glucose homeostasis in lean or obese mice. Our work demonstrates the opposite, as acute elevations in circulating ketones improved glucose tolerance in obese mice.
The Efficacy of Manual Therapy and Pressure Biofeedback-Guided Deep Cervical Flexor Muscle Strength Training on Pain and Functional Limitations in Individuals with Cervicogenic Headaches: A Randomized Comparative Study
Authors: Hasan Shahnaz , Bharti Nasrin , Alghadir Ahmad H. , Iqbal Amir , Shahzad Naiyer , Ibrahim Abeer R.
Published: August 2023

Objective. This study aimed to compare the efficacy of manual therapy and pressure biofeedback-guided DCFM strength training on pain intensity and functional limitations in individuals with CGH. Trial Design. A double-blinded, two-arm parallel group randomized comparative design. Methods. After applying the eligibility criteria, sixty out of eighty-nine CGH patients were recruited from King Saud University Medical Center in Riyadh and randomly allocated to intervention groups using simple random sampling. Group 1 underwent pressure biofeedback-guided DCFM strength training and conventional treatment, while Group 2 received manual therapy and conventional treatment for three consecutive weeks. The main outcome measures were scores on the visual analog scale (VAS) and the headache disability index (HDI). One assessor and two physical therapists were blinded to group allocation. Results. Sixty out of eighty participants aged 29–40 years were randomized into intervention groups (n = 30/group; age (mean ± standard deviation): group 1 = 35.0 ± 2.82; group 2 = 34.87 ± 2.60), and their data were analyzed. A significant improvement (95% CI,

p
<
0.05

) was observed within each group when comparing the VAS and HDI scores between baseline and postintervention. In contrast, between-group comparisons for the outcome score of VAS and HDI revealed nonsignificant differences in the first, second, and third weeks after intervention, except for the VAS score, which showed a significant difference in weeks 2 and 3 after intervention. Cohen’s d-value indicated that the intervention effect size for reducing pain was larger in group 1 than in group 2 at weeks 2 and 3. Conclusion. Compared with manual therapy, pressure biofeedback-guided DCFM strength training showed a greater reduction in pain intensity (assessed using the VAS) at weeks two and three. However, both treatments were equally effective in lowering headache-related functional limitations in patients with CGH. This trial is registered with ClinicalTrial.gov PRS (Identifier ID: NCT05692232).
Plant Growth Promotion and Biocontrol Potentiality of Endophytes Isolated from Root Nodules of Sulla flexuosa L. Plants
Authors: Hamane Samia , El Yemlahi Anass , Hassani Zerrouk Mounir , El Galiou Ouiam , Laglaoui Amin , Bakkali Mohammed , Arakrak Abdelhay
Published: August 2023

Legumes, native to the Mediterranean, harbor reservoirs of endophytes that help plants adapt to various environmental stresses. The current study was carried out to evaluate the plant growth characteristics and antifungal activity of root nodule endophytes as biocontrol agents and plant growth promoters. Eleven bacterial endophytes isolated from root nodules of Sulla flexuosa L. grown in Northwest Morocco were assessed for their plant growth-promoting (PGP), and antifungal properties. Four endophytic bacteria were selected for their efficiency in solubilizing inorganic phosphate. The selected strains were positive for more than 2 PGP traits, including indole acetic acid, ACC deaminase, siderophore, and ammonia production. The screening for lytic enzyme production revealed that all strains were capable of producing chitinase, cellulase, catalase, and protease, while the secretion of amylase and urease was not detected. The HFB11 was the only strain incapable of producing pectinase. In vitro experiments revealed the strains’ potential to withstand salt and drought stresses by being able to grow in high concentrations of NaCl and PEG. Based on 16S rRNA gene sequencing, the strains were identified as Enterobacter and Serratia. The antagonistic activity of the strains against Botrytis cinerea, Aspergillus ochraceus, and Fusarium oxysporum was detected and they were shown to inhibit the fungal growth with various percentages. The highest percentage of inhibition was observed for HFB3 against B. cinerea with 50% inhibition followed by HFB8 which was able to inhibit 47% of F. oxysporum’s growth. In contrast, a weak inhibition was observed against A. ochraceus. All these findings indicate that the chosen endophytes, halotolerant Serratia inhibens HFB8 and Enterobacter hormaechei HFB11, might be used as candidates for effective biocontrol and growth promotion of legumes.
Vasoprotective Effects of Hyperoside against Cerebral Ischemia/Reperfusion Injury in Rats: Activation of Large-Conductance Ca2+-Activated K+ Channels
Authors: Hong Wen-Ming , Xie Yue-Wu , Zhao Meng-Yu , Yu Tian-Hang , Wang Li-Na , Xu Wan-Yan , Gao Shen , Cai Hua-Bao , Guo Yan , Zhang Fang
Published: August 2023

Hyperoside (Hyp), a kind of Chinese herbal medicine, exerts multiple therapeutic effects on many diseases. However, the role and mechanisms of Hyp in vascular pathophysiology in ischemic stroke need to be further established. The study aimed to investigate the role of (large-conductance Ca2+-activated K+) BK channels on the vasoprotection of Hyp against cerebral ischemia and reperfusion (I/R) injury in rats. The concentration gradient of Hyp was pretreated in both the middle cerebral artery occlusion and reperfusion model and oxygen-glucose deprivation/reoxygenation (OGD/R) model of primary vascular smooth muscle cells (VSMCs) in rats. A series of indicators were detected, including neurological deficit score, infarct volume, malondialdehyde (MDA), superoxide dismutase (SOD), cerebral blood flow (CBF), cell viability, membrane potential, and BK channels α- and β1-subunits expression. The results showed that Hyp significantly reduced infarct volume and ameliorated neurological dysfunction in I/R-injured rats. Besides, the effects of I/R-induced reduction of BK channels α- and β1-subunits expression were significantly reversed by Hyp in endothelial-denudated cerebral basilar arteries. Furthermore, the protective effect against I/R-induced increases of MDA and reduction of SOD as well as CBF induced by Hyp was significantly reversed by iberiotoxin (IbTX). In OGD/R-injured VSMCs, downregulated cellular viability and BK channels β1-subunits expression were remarkably reversed by Hyp. However, neither OGD/R nor Hyp affected BK channels α-subunits expression, and Hyp failed to induced hyperpolarization of VSMCs. Moreover, the protective effect against OGD/R-induced reduction of cell viability and SOD level and increases of MDA production induced by Hyp was significantly reversed by IbTX in VSMCs. The study indicates that Hyp has the therapeutic potential to improve vascular outcomes, and the mechanism is associated with suppressing oxidative stress and improving CBF through upregulating BK channels.
Investigation on Three-Body Abrasion Resistance of Mild Steel Soil Slurry Condition-Simulating Agricultural Condition
Authors: Alsaeed Talal , Eid Alajmi Ayedh , Ghanem Alotaibi Jasem , Yousif Belal F.
Published: August 2023

The agricultural industry heavily relies on machinery and equipment for efficient farming practices, but harsh environmental conditions can cause premature wear and tear, leading to financial losses. Three-body abrasion research is essential for developing more durable materials for various industries, including agriculture. Mild steel is commonly used in agricultural machinery but lacks resistance to moisture and corrosion. Slurry handling equipment is prone to wear due to abrasive particles, and red soil presents challenges for farmers. A study was conducted to investigate the wear resistance of mild steel under three-body abrasion under slurry conditions, simulating real-life agricultural environments. Different loads and operating durations were considered, and SEM was used to examine the worn surfaces. Weight loss during sliding wear was found to increase proportionally with duration and applied load. Increasing load leads to more severe wear due to higher shear forces and the formation of nucleation sites for wear particles. There is an increase in the weight loss by about 8 times when the applied load increased from 10 N to 70 N. The slurry regime and rotational speeds also affect wear rate, with higher speeds leading to deeper penetration of abrasive particles and greater impact force. The severity of wear increases with time and different wear mechanisms dominating at different durations, as observed through the SEM analysis. These findings emphasize the importance of considering load, slurry regime, and rotational speed when designing materials for sliding wear applications.
Short-Term Inbound and Outbound Passenger Flow Prediction for New Metro Stations Based on Clustering and Deep Learning
Authors: Wang Zihe , Zhang Yongsheng , Yao Enjian , Wang Yue , Li Juncheng , He Jiantao
Published: August 2023

The rapid expansion of metro networks, e.g., in many cities of China, continuously introduces the operation of new stations every year. Due to the lack of historical data and complicate variations of short-term passenger flow in the early stage of operation, it is difficult to accurately predict inbound and outbound passenger flows of new metro stations in the short term, which would be the database for train scheduling for new stations before operation, dynamic capacity optimization for new stations under operation, short-term prediction of cycle sharing demands near new stations, and so on. Traditional methods usually failed to exactly reflect the complicate rules or were unusable without the new station’s historical data. In order to solve the above problems, this paper proposes a short-term inbound and outbound passenger flow prediction model for new metro stations at the early stage of operation by combining the K-means clustering algorithm, an improved spatiotemporal long short-term memory model (Sp-LSTM), and a real-time feedback error model (mean absolute error, MAE), where passenger flows’ spatial-temporal characteristics and land-use relevance are considered. The application in Guangzhou Metro, China, where Line 21 is regarded as a new line, shows that the proposed K-Sp-LSTM model has the best prediction accuracy compared with traditional methods.
Assessment of Acrylamide Formation in Various Iraqi Bread Types and Mitigation of Acrylamide Production by Calcium Carbonate in White Flatbread
Authors: Alsharafani Mustafa A. M. , Alhendi Abeer S. , Sharif Taghreed K. , Ahmed Hassan Abdulwahed , Jawad Ali M. , Alsallami Rana T. , Abdulmawjood Amir , Khan Izhar U. H.
Published: August 2023

Acrylamide is a neuro- and reproductive toxicant to humans and has carcinogenic effects in animal species which naturally forms in high-carbohydrate foods at high (≤121°C) temperatures. This study assessed acrylamide formation in various Iraqi bread types, including whole-wheat flatbread, white flatbread, stone-baked bread, whole-wheat baguette, and white baguette prepared in the selected local bread factories in Baghdad, Iraq, using HPLC assay. Among several types of bread, white flatbread is the most commonly consumed bread in Iraq; therefore, the impact of calcium carbonate (CaCO3), added to the flour at different concentrations: 240, 260, and 280 mg/100 g, in reducing acrylamide concentration only in white flatbread was assessed. Acrylamide was detected at various concentrations in each type of bread tested, with the highest concentration in whole-wheat flatbread (470.2 ± 6.7 μg/kg) followed by stone-baked bread (418.8 ± 6.4 μg/kg), whole-wheat baguette (408.3 ± 9.1 μg/kg), white flatbread (400.9 ± 7.4 μg/kg), and white baguette (362 ± 9.2 μg/kg), respectively. However, after supplementing CaCO3 in white flatbread, a significant

p
<
0.05

reduction in acrylamide (219 ± 8.5, 121.8 ± 1.7, and 115 ± 3.4 μg/kg, for the three calcium carbonate concentrations) as compared to the control (308.3 ± 2.9 μg/kg) was observed without impacting the rheological properties of bread. In conclusion, the acrylamide concentration can be reduced by supplementing CaCO3 in the white flatbread without compromising the rheological properties (i.e., dough strength, volume, and water absorption) and overall bread quality. The study results would help to mitigate the risk of toxic effects in humans.
Deformation and Fracturing Behaviors of Sandstone Disc Containing a Circular Inclusion under Diametrical Compression: Insights from Integrated SG and DIC Experiments
Authors: Nie Rongshan , Wu Qiuhong , Chu Zhaofei , Weng Lei
Published: August 2023

Weak inclusions play a significant role in regulating the deformation and fracturing behavior of rock masses. In this study, sandstone specimens filled with circular inclusions were subjected to diametrical compression until failure. The local strain concentration intensity and cracking process at the rock-inclusion interface were specifically investigated using the integrated strain gauge (SG) and digital image correlation (DIC) techniques. The experimental results reveal that the presence of inclusions introduces more complex deformation and fracturing behaviors in the infilled sandstone specimens. The stiffness of the infilled sandstones, with either low-strength or high-strength inclusions, initially increases and then decreases as the inclusion diameter increases. The effective load-carrying capacity of the infilled sandstone is influenced by the competing effects of inclusion enhancement and hole weakening. Moreover, a more pronounced strain concentration occurs at the inclusion boundary when the inclusion diameter is larger and the inclusion strength is lower. The crack mode in the infilled red sandstone is independent of the strength of the inclusion. For larger hole diameters, fracture planes form along the compression line, whereas interfacial debonding occurs in red sandstone with smaller hole diameters. Finally, the stress distribution characteristics along the loaded diameter and transverse diameter were analyzed using elastic finite element analyses, which can well explain the cracking mechanism of the infilled disc specimens.
Supplementary Figure S4 from Integrated Multimodal Analyses of DNA Damage Response and Immune Markers as Predictors of Response in Metastatic Triple-Negative Breast Cancer in the TNT Trial (NCT00532727)
Authors: Tovey Holly , Sipos Orsolya , Parker Joel S. , Hoadley Katherine A. , Quist Jelmar , Kernaghan Sarah , Kilburn Lucy , Salgado Roberto , Loi Sherene , Kennedy Richard D. , Roxanis Ioannis , Gazinska Patrycja , Pinder Sarah E. , Bliss Judith , Perou Charles M. , Haider Syed , Grigoriadis Anita , Tutt Andrew , Cheang Maggie Chon U
Published: August 2023

This is an example of DDR-deficient case with high TILs and low gene-expression measurements. These cases were confirmed to have high TIL content (black delineation) and are characterized by both high tumour area- stromal area ratio as well as a high tumour cell- stromal cell ratio. Moreover, all these cases were characterized by high grade features, such as necrosis (blue delineation), high mitotic activity (green arrow), and high levels of atypia (blue arrow), and all had a solid growth pattern, with no formation of glands.
Supplementary Figure S3 from Integrated Multimodal Analyses of DNA Damage Response and Immune Markers as Predictors of Response in Metastatic Triple-Negative Breast Cancer in the TNT Trial (NCT00532727)
Authors: Tovey Holly , Sipos Orsolya , Parker Joel S. , Hoadley Katherine A. , Quist Jelmar , Kernaghan Sarah , Kilburn Lucy , Salgado Roberto , Loi Sherene , Kennedy Richard D. , Roxanis Ioannis , Gazinska Patrycja , Pinder Sarah E. , Bliss Judith , Perou Charles M. , Haider Syed , Grigoriadis Anita , Tutt Andrew , Cheang Maggie Chon U
Published: August 2023

Heatmap of signatures used for clustering by the resulting novel clusters.
Data from Integrated Multimodal Analyses of DNA Damage Response and Immune Markers as Predictors of Response in Metastatic Triple-Negative Breast Cancer in the TNT Trial (NCT00532727)
Authors: Tovey Holly , Sipos Orsolya , Parker Joel S. , Hoadley Katherine A. , Quist Jelmar , Kernaghan Sarah , Kilburn Lucy , Salgado Roberto , Loi Sherene , Kennedy Richard D. , Roxanis Ioannis , Gazinska Patrycja , Pinder Sarah E. , Bliss Judith , Perou Charles M. , Haider Syed , Grigoriadis Anita , Tutt Andrew , Cheang Maggie Chon U
Published: August 2023

AbstractPurpose:
The TNT trial (NCT00532727) showed no evidence of carboplatin superiority over docetaxel in metastatic triple-negative breast cancer (mTNBC), but carboplatin benefit was observed in the germline BRCA1/2 mutation subgroup. Broader response-predictive biomarkers are needed. We explored the predictive ability of DNA damage response (DDR) and immune markers.
Patients and Methods:
Tumor-infiltrating lymphocytes were evaluated for 222 of 376 patients. Primary tumors (PT) from 186 TNT participants (13 matched recurrences) were profiled using total RNA sequencing. Four transcriptional DDR-related and 25 immune-related signatures were evaluated. We assessed their association with objective response rate (ORR) and progression-free survival (PFS). Conditional inference forest clustering was applied to integrate multimodal data. The biology of subgroups was characterized by 693 gene expression modules and other markers.
Results:
Transcriptional DDR-related biomarkers were not predictive of ORR to either treatment overall. Changes from PT to recurrence were demonstrated; in chemotherapy-naïve patients, transcriptional DDR markers separated carboplatin responders from nonresponders (P values = 0.017; 0.046). High immune infiltration was associated with docetaxel ORR (interaction P values < 0.05). Six subgroups were identified; the immune-enriched cluster had preferential docetaxel response [62.5% (D) vs. 29.4% (C); P = 0.016]. The immune-depleted cluster had preferential carboplatin response [8.0% (D) vs. 40.0% (C); P = 0.011]. DDR-related subgroups were too small to assess ORR.
Conclusions:
High immune features predict docetaxel response, and high DDR signature scores predict carboplatin response in treatment-naïve mTNBC. Integrating multimodal DDR and immune-related markers identifies subgroups with differential treatment sensitivity. Treatment options for patients with immune-low and DDR-proficient tumors remains an outstanding need. Caution is needed using PT-derived transcriptional signatures to direct treatment in mTNBC, particularly DDR-related markers following prior chemotherapy.
Supplementary Table 2 from Resistance to Selective FGFR Inhibitors in FGFR-Driven Urothelial Cancer
Authors: Facchinetti Francesco , Hollebecque Antoine , Braye Floriane , Vasseur Damien , Pradat Yoann , Bahleda Rastislav , Pobel Cédric , Bigot Ludovic , Déas Olivier , Florez Arango Juan David , Guaitoli Giorgia , Mizuta Hayato , Combarel David , Tselikas Lambros , Michiels Stefan , Nikolaev Sergey I. , Scoazec Jean-Yves , Ponce-Aix Santiago , Besse Benjamin , Olaussen Ken A. , Loriot Yohann , Friboulet Luc
Published: August 2023

Systemic treatment received by patients prior to selective FGFR inhibitors, according to the detection of FGFR kinase domain mutations and/or PI3K/mTOR pathway alterations.
Supplementary Figure 4 from Resistance to Selective FGFR Inhibitors in FGFR-Driven Urothelial Cancer
Authors: Facchinetti Francesco , Hollebecque Antoine , Braye Floriane , Vasseur Damien , Pradat Yoann , Bahleda Rastislav , Pobel Cédric , Bigot Ludovic , Déas Olivier , Florez Arango Juan David , Guaitoli Giorgia , Mizuta Hayato , Combarel David , Tselikas Lambros , Michiels Stefan , Nikolaev Sergey I. , Scoazec Jean-Yves , Ponce-Aix Santiago , Besse Benjamin , Olaussen Ken A. , Loriot Yohann , Friboulet Luc
Published: August 2023

Expression of FGFR3:TACC3 was homogeneous across Ba/F3 cell lines.
Supplementary Figure 3 from Resistance to Selective FGFR Inhibitors in FGFR-Driven Urothelial Cancer
Authors: Facchinetti Francesco , Hollebecque Antoine , Braye Floriane , Vasseur Damien , Pradat Yoann , Bahleda Rastislav , Pobel Cédric , Bigot Ludovic , Déas Olivier , Florez Arango Juan David , Guaitoli Giorgia , Mizuta Hayato , Combarel David , Tselikas Lambros , Michiels Stefan , Nikolaev Sergey I. , Scoazec Jean-Yves , Ponce-Aix Santiago , Besse Benjamin , Olaussen Ken A. , Loriot Yohann , Friboulet Luc
Published: August 2023

Acquisition of mutations in PI3K/TSC1 pathway at progression to FGFR inhibitors.
Supplementary Figure 2 from Resistance to Selective FGFR Inhibitors in FGFR-Driven Urothelial Cancer
Authors: Facchinetti Francesco , Hollebecque Antoine , Braye Floriane , Vasseur Damien , Pradat Yoann , Bahleda Rastislav , Pobel Cédric , Bigot Ludovic , Déas Olivier , Florez Arango Juan David , Guaitoli Giorgia , Mizuta Hayato , Combarel David , Tselikas Lambros , Michiels Stefan , Nikolaev Sergey I. , Scoazec Jean-Yves , Ponce-Aix Santiago , Besse Benjamin , Olaussen Ken A. , Loriot Yohann , Friboulet Luc
Published: August 2023

Documentation of the allelic status in cis of FGFR3 S249C and V553L mutations from MR406 tissue biopsy.
Table S7 from Single-cell profiling uncovers a Muc4-expressing metaplastic gastric cell type sustained by Helicobacter pylori-driven inflammation
Authors: O'Brien Valerie Phoebe , Kang Yuqi , Shenoy Meera K , Finak Greg , Young William C , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M , Koch Meghan A , Gottardo Raphael , Salama Nina R.
Published: August 2023

Expression of the mucin MUC4 and the cell proliferation marker Ki-67 in each specimen in the gastric cancer tissue microarray (TMA).
Table S1 from Single-cell profiling uncovers a Muc4-expressing metaplastic gastric cell type sustained by Helicobacter pylori-driven inflammation
Authors: O'Brien Valerie Phoebe , Kang Yuqi , Shenoy Meera K , Finak Greg , Young William C , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M , Koch Meghan A , Gottardo Raphael , Salama Nina R.
Published: August 2023

Description of the mouse samples used for single-cell RNA-sequencing.
Figure S6 from Single-cell profiling uncovers a Muc4-expressing metaplastic gastric cell type sustained by Helicobacter pylori-driven inflammation
Authors: O'Brien Valerie Phoebe , Kang Yuqi , Shenoy Meera K , Finak Greg , Young William C , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M , Koch Meghan A , Gottardo Raphael , Salama Nina R.
Published: August 2023

Spatial profiling reveals enrichment of Muc4 and Areg expression from the luminal surface in the Hp+KRAS+ gastric epithelium.
Figure S3 from Single-cell profiling uncovers a Muc4-expressing metaplastic gastric cell type sustained by Helicobacter pylori-driven inflammation
Authors: O'Brien Valerie Phoebe , Kang Yuqi , Shenoy Meera K , Finak Greg , Young William C , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M , Koch Meghan A , Gottardo Raphael , Salama Nina R.
Published: August 2023

Genes driving the subclustering of the major gastric epithelial cell types.
Figure S13 from Single-cell profiling uncovers a Muc4-expressing metaplastic gastric cell type sustained by Helicobacter pylori-driven inflammation
Authors: O'Brien Valerie Phoebe , Kang Yuqi , Shenoy Meera K , Finak Greg , Young William C , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M , Koch Meghan A , Gottardo Raphael , Salama Nina R.
Published: August 2023

MUC4 expression was not associated with sex or age in our cohort of 47 gastric cancer cases seen in the United States Pacific Northwest.
Figure S10 from Single-cell profiling uncovers a Muc4-expressing metaplastic gastric cell type sustained by Helicobacter pylori-driven inflammation
Authors: O'Brien Valerie Phoebe , Kang Yuqi , Shenoy Meera K , Finak Greg , Young William C , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M , Koch Meghan A , Gottardo Raphael , Salama Nina R.
Published: August 2023

Gating strategy for detection of T cell populations by flow cytometry.
Supplementary Table S6 from Cancer Epidemiology in the Northeastern United States (2013–2017)
Authors: Rees Judy R. , Weiss Julie E. , Gunn Christine M. , Carlos Heather A. , Dragnev Nathalie C. , Supattapone Emma Y. , Tosteson Anna N.A. , Kraft Sally A. , Vahdat Linda T. , Peacock Janet L.
Published: August 2023

Northern New England state and population characteristics
Supplementary Figure 2 from Cancer Epidemiology in the Northeastern United States (2013–2017)
Authors: Rees Judy R. , Weiss Julie E. , Gunn Christine M. , Carlos Heather A. , Dragnev Nathalie C. , Supattapone Emma Y. , Tosteson Anna N.A. , Kraft Sally A. , Vahdat Linda T. , Peacock Janet L.
Published: August 2023

Average Annual Percent Change (APC) in Age-Adjusted Cancer Incidence (2014-2018); Figure 2b Average Annual Percent Change (APC) in Age-Adjusted Cancer Mortality (2014-18)
FIGURE 4 from Cancer Epidemiology in the Northeastern United States (2013–2017)
Authors: Rees Judy R. , Weiss Julie E. , Gunn Christine M. , Carlos Heather A. , Dragnev Nathalie C. , Supattapone Emma Y. , Tosteson Anna N.A. , Kraft Sally A. , Vahdat Linda T. , Peacock Janet L.
Published: August 2023

Ratios of age-standardized* cancer mortality rates (RR) and 99% CIs† for ME, NH, VT, and U.S. regions compared with United States, overall and among non-Hispanic White 2013–2017.
FIGURE 3 from Cancer Epidemiology in the Northeastern United States (2013–2017)
Authors: Rees Judy R. , Weiss Julie E. , Gunn Christine M. , Carlos Heather A. , Dragnev Nathalie C. , Supattapone Emma Y. , Tosteson Anna N.A. , Kraft Sally A. , Vahdat Linda T. , Peacock Janet L.
Published: August 2023

Leading cancer sites ratio of age-standardized* incidence rates (RR) and 99% CIs† for combined ME, NH, VT versus the United States, by race/ethnicity 2013–2017.
FIGURE 1 from Cancer Epidemiology in the Northeastern United States (2013–2017)
Authors: Rees Judy R. , Weiss Julie E. , Gunn Christine M. , Carlos Heather A. , Dragnev Nathalie C. , Supattapone Emma Y. , Tosteson Anna N.A. , Kraft Sally A. , Vahdat Linda T. , Peacock Janet L.
Published: August 2023

Derivation of cancer cases.
Data from Single-cell profiling uncovers a Muc4-expressing metaplastic gastric cell type sustained by Helicobacter pylori-driven inflammation
Authors: O'Brien Valerie Phoebe , Kang Yuqi , Shenoy Meera K , Finak Greg , Young William C , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M , Koch Meghan A , Gottardo Raphael , Salama Nina R.
Published: August 2023

Abstract
Mechanisms for Helicobacter pylori (Hp)-driven stomach cancer are not fully understood. In a transgenic mouse model of gastric preneoplasia, concomitant Hp infection and induction of constitutively active KRAS (Hp+KRAS+) alters metaplasia phenotypes and elicits greater inflammation than either perturbation alone. Gastric single-cell RNA-seq showed that Hp+KRAS+ mice had a large population of metaplastic pit cells that expressed the intestinal mucin Muc4 and the growth factor amphiregulin. Flow cytometry and immunohistochemistry-based immune profiling revealed that metaplastic pit cells were associated with macrophage and T cell inflammation. Accordingly, expansion of metaplastic pit cells was prevented by gastric immunosuppression and reversed by antibiotic eradication of Hp. Finally, MUC4 expression was significantly associated with proliferation in human gastric cancer samples. These studies identify an Hp-associated metaplastic pit cell lineage, also found in human gastric cancer tissues, whose expansion is driven by Hp-dependent inflammation.
Campylobacter Colitis as a Trigger for Atypical Hemolytic Uremic Syndrome: About One Case
Authors: Quinaux Thomas , Tubail Zead , Vrillon Isabelle , Sartelet Hervé , Savenkoff Benjamin
Published: August 2023

We present the case of a 17-year-old Caucasian male whose condition featured acute renal failure, anemia, and deep thrombocytopenia after five consecutive days of diarrhea. Campylobacter coli was identified in stool cultures and, although the direct role of this germ in the pathogenesis of hemolytic uremic syndrome (HUS) remains uncertain to this day, initial presentation was considered broadly consistent with typical HUS. However, the patient showed no signs of spontaneous recovery over time. While secondary investigations showed no abnormalities in ADAMTS13 activity or in the alternate pathway of complement, patient’s condition deteriorated. Worsening kidney failure required emergency renal replacement therapy and was followed by cardiac involvement in the form of acute heart failure. Given this unfavorable development, blood samples were drawn to look for mutations in the alternate complement pathway, and eculizumab therapy was initiated without further delay, allowing prompt improvement of cardiac function and recovery of diuresis. Upon discharge, the patient still had to undergo intermittent dialysis, which would later be withdrawn. Genetic analysis ultimately confirmed the presence of a complement factor H mutation associated with a high risk of disease recurrence, indicating long-term continuation of eculizumab therapy.
Use of Immunohistochemical p53 Mutant-Phenotype in Diagnosis of High-Grade Dysplasia of Esophageal Squamous Epithelia
Authors: Xu Yan-Juan , Li Ran , Chen Jiang-Mu , Zhuang Xue-Yu , Lin Na , Wang Lin-Pei , Zeng Bing-Wei
Published: August 2023

Background: Mild cellular atypia of esophageal squamous epithelial dysplasia has a risk of progressing to cancer that poses great confusion for pathological diagnosis. There is no research on the diagnosis and differential diagnosis of esophageal squamous dysplasia by the expression of immunohistochemical (IHC) p53. The study aims to conduct a graded diagnosis of esophageal squamous epithelial hyperplasia by combining p53 expressions and microscopic histomorphological characteristics. Methods: The study was conducted from January 2021 to January 2022 and included a total of 208 cases including 262 specimens with atypical hyperplasia or dysplasia of squamous epithelia discovered by esophageal mucosal biopsy. HE staining was used to grade the epithelial hyperplasia degree, and all cases underwent p53 IHC evaluation. Results: Benign lesions: we did not find any p53 IHC mutant-phenotype (0/12 cases) in 12 cases of esophagitis. We found 10 cases (10/80 cases) of p53 IHC mutant-phenotype in 80 cases of low-grade dysplasia, and 158 cases (158/170 cases) of p53 IHC mutant-phenotype of high-grade lesions in 170 cases of high-grade dysplasia and early cancer based on the χ2 test results. We found statistically significant differences in p53 IHC mutant-phenotype between the high-grade squamous epithelial lesions and benign lesions. The sensitivity and specificity of p53 in detecting high-grade squamous epithelial lesions were 92.9% and 89.1%, respectively. The positive predictive value was 94.0%, and the negative predictive value was 87.2%. Conclusion: In this study, we found that p53 IHC had high sensitivity and specificity in detecting high-grade esophageal squamous epithelial lesions. Therefore, it has potential to be used as a routine item in pathological detection for auxiliary risk stratification of esophageal squamous epithelial lesions.
U-Shaped Relationship between Sleep Duration and CKD in US Adults: Data from National Health and Nutrition Examination Survey (NHANES) 2005–2014
Authors: Jiang Luojia , Xu Haibo
Published: August 2023

Introduction: Short and long sleep durations have been associated with adverse health outcomes. The objective of this study was to examine the association between self-reported sleep duration and chronic kidney disease (CKD) in the general population on the basis of the National Health and Nutrition Examination Survey (NHANES) database. Methods: A total of 28,239 adults aged ≥18 years who participated in the 2005–2014 NHANES were analyzed. CKD was defined as an estimated glomerular filtration rate <60 mL/min per 1.73 m2 or urinary albumin/urine creatinine ratio ≥300 mg/g. Very short sleepers and short sleepers were defined as those who sleep ≤5 h or 5.1–6.9 h per day, respectively. Long sleepers and very long sleepers were defined as those who sleep 9.0–10.9 h or ≥11 h per day, respectively. Normal sleepers were defined as those who sleep 7.0–8.9 h. The association between sleep duration and CKD was assessed using a logistic regression model. Results: Very short (≤5 h) sleep duration was associated with higher odds of CKD (multiadjusted OR, 1.38; 95% confidence interval, 1.17–1.62 comparing normal categories of 7.0–8.9 h; P trend = 0.01), after adjusting for potential confounders. Participants with long (9–10.9 h) sleep duration also tended to have a higher odds of CKD (multiadjusted OR, 1.39; 95% confidence interval, 1.20–1.61 comparing normal categories of 7.0–8.9 h; P trend = 0.01). This risk was further increased in those whose sleep duration exceeded 11 h (multiadjusted OR, 2.35; 95% confidence interval, 1.64–3.37 comparing normal categories of 7.0–8.9; P trend = 0.01). However, there was no statistically significant association between short (≤6.0–7.9 h) sleep duration and CKD (multiadjusted OR, 1.05; 95% confidence interval, 0.96–1.14 comparing normal categories of 7.0–8.9 h; P trend = 0.32). Conclusion: We demonstrated that the higher CKD prevalence estimates were found in very short (≤5 h) and long (9.0–10.9 h) sleep durations in an apparently healthy population aged ≥18 years in the USA. This prevalence of CKD is further increased in those whose sleep duration exceeds 11 h. Our cross-sectional analyses clarified the U-shaped temporal relationship between sleep duration and CKD.
Haploidentical Stem Cell Transplantation Using Post-Transplant Cyclophosphamide for T-Cell Prolymphocytic Leukemia after Alemtuzumab Induction Therapy: A Case Report
Authors: Okada Naoki , Yamamoto Ryusuke , Maruoka Hayato , Himeno Mayuko , Hiramoto Nobuhiro , Ishikawa Takayuki
Published: August 2023

T-cell prolymphocytic leukemia (T-PLL) is a rare aggressive disease with a poor prognosis. Allogeneic stem cell transplantation (allo-SCT) followed by alemtuzumab administration is the most promising treatment for T-PLL but is associated with a high risk of infections as alemtuzumab strongly suppresses cellular immunity, leading to high transplant-related mortality and unsatisfactory survival rates. In addition, for patients without human leukocyte antigen-matched donors, haploidentical stem cell transplantation (haplo-SCT) using post-transplant cyclophosphamide (PTCy) has been used because of the ready availability of donors and achievement of results comparable to those of transplantation with human leukocyte antigen-matched donors. However, there are no reports on the efficacy and safety, including infectious complications, of haplo-SCT with PTCy after alemtuzumab therapy in patients with. Here, we describe a 66-year-old Japanese male patient with T-PLL treated successfully with haplo-SCT after induction therapy of alemtuzumab for T-PLL. Approximately 3 months after the achievement of complete remission with alemtuzumab for T-PLL, haplo-SCT with reduced-intensity conditioning and PTCy was performed. Infectious complications were improved by early therapeutic interventions, and peripheral T cell counts gradually recovered. The patient was alive for more than 16 months after allo-SCT with no signs of relapse. Thus, haplo-SCT using PTCy should be considered as an option after alemtuzumab treatment for T-PLL.
The Chronology of Renal Allograft Dysfunction: The Pathological Perspectives
Authors: Hara Shigeo
Published: August 2023

Background: Antibody-mediated rejection (ABMR), T-cell-mediated rejection (TCMR), BK polyomavirus nephropathy, and calcineurin inhibitor (CNI) toxicity are all common causes of kidney allograft dysfunction that can affect long-term allograft function. Summary: The prevalence of various pathological diagnoses changes over time for both indication and protocol biopsies. Active ABMR and CNI toxic tubulopathy are the leading causes of kidney allograft dysfunction in the early posttransplant period. Active ABMR can also manifest as thrombotic microangiopathy. Acute TCMR, borderline for acute TCMR, and BK polyomavirus nephropathy will occur, then comes a causal peak of renal allograft dysfunction, followed by chronic active ABMR. Active ABMR in the late posttransplant period would progress to chronic active ABMR, indicating sequential evolution from the incipient to advanced phase of chronic active ABMR. CNI toxicity also manifests as chronic lesions of arteriolar hyalinosis. Interstitial fibrosis and tubular atrophy are the result of multiple insults and are linked to underlying diseases, particularly in the late posttransplant period. Even with established pathological criteria of the Banff scheme, it can be still challenging to clearly delineate the causes of the allograft dysfunction, especially in the complicated cases. Understanding the chronological causes of renal allograft dysfunctions improves comprehension of renal allograft pathology. Key Messages: Identifying the time-dependent prevalence of renal allograft dysfunction can be a critical and effective approach to pathological diagnosis.
Retrospective Evaluation of Cytogenetic Effects Induced by Internal Radioiodine Exposure: A 27-Year Follow-Up Study
Authors: Livingston Gordon K. , Ryan Terri L. , Escalona Maria B. , Foster Alvis E. , Balajee Adayabalam S.
Published: August 2023

Radioiodine (131I) is widely used in the treatment of hyperthyroidism and as an effective ablative therapy for differentiated thyroid cancer. Radioiodine (131I) constitutes 90% of the currently used therapies in the field of nuclear medicine. Here, we report the cytogenetic findings of a long-term follow-up study of 27 years on a male patient who received two rounds of radioiodine treatment within a span of 26 months between 1992 and 1994 for his papillary thyroid cancer. A comprehensive cytogenetic follow-up study utilizing cytokinesis blocked micronucleus assay, dicentric chromosome assay, genome wide translocations and inversions was initiated on this patient since the first administration of radioiodine in 1992. Frequencies of micronuclei (0.006/cell) and dicentric chromosomes (0.008/cell) detected in the current study were grossly similar to that reported earlier in 2019. The mFISH analysis detected chromosome aberrations in 8.6% of the cells in the form of both unbalanced and balanced translocations. Additionally, a clonal translocation involving chromosomes 14p; 15q was observed in 2 of the 500 cells analyzed. Out of the 500 cells examined, one cell showed a complex translocation (involving chromosomes 9, 10, and 16) besides 5 other chromosome rearrangements. Collectively, our study indicates that the past radioiodine exposure results in long-lasting chromosome damage and that the persistence of translocations can be useful for both retrospective biodosimetry and for monitoring chromosome instability in the lymphocytes of radioiodine exposed individuals.
Correlation of Baseline Tumor Burden with Clinical Outcome in Melanoma Patients Treated with Ipilimumab
Authors: Angelova-Toshkina Daniela , Weide Benjamin , Tietze Lutz F. , Hebst Michelle , Tietze Julia K.
Published: August 2023

Introduction: Tumor burden is a frequently mentioned parameter; however, a commonly accepted definition is still lacking. Methods: In this double-center prospective and retrospective study, 76 patients with unresectable stage III or stage IV melanoma treated with ipilimumab were included. We defined the baseline tumor burden (BTB) as the global sum of all metastases’ longest diameters before treatment started and correlated the calculated BTB with disease control rate (DCR), progression-free survival (PFS), overall survival (OS), and with the baseline levels of LDH, S100B, and sULPB2. Results: BTB correlated significantly with DCR (p = 0.009), PFS (p = 0.002), OS (p = 0.032), and the occurrence of NRAS mutation (p = 0.006). BTB was also correlated to baseline serum levels of LDH (p = 0.011), S100B (p = 0.027), and SULBP (p < 0.0001). Multivariate analysis revealed that BPB and LDH were independently correlated with PFS and OS. With increasing BTB, disease control was less likely; no patient with a BTB >200 mm achieved disease control. For patients with brain metastasis, no correlation of BTB with DCR (p = 0.251), PFS (p = 0.059), or OS (p = 0.981) was observed. Conclusion: Calculated BTB is an independent prognostic factor for patients with metastatic melanoma treated with ipilimumab. Using calculated BTB as a definition of tumor burden may help increase comparability of outcome of therapies in future studies.
Asthma bronchiale – ein Karrierekiller?
Authors: Velasco Garrido Marcial , Preisser Alexandra M.
Published: August 2023

Objectives: Asthma has significant occupational consequences. The objective of our study was to investigate the links between asthma and the career path, taking into account gender and age at asthma onset. Methods: Using cross-sectional data collected at inclusion in the French CONSTANCES cohort in 2013-2014, we studied the links between each career path indicator (number of job periods, total duration of employment, numbers of part-time jobs and work interruptions due to unemployment or health issues, employment status at inclusion) on the one hand, and current asthma and asthma symptom score in the last 12 months on the other hand, as reported by the participants. Multivariate analyses were performed separately for men and women using logistic and negative binomial regression models adjusted for age, smoking status, body mass index and educational level. Results: When the asthma symptom score was used, significant associations were observed with all of the career path indicators studied: a high symptom score was associated with a shorter total duration of employment as well as a greater number of job periods, part-time jobs and work interruptions due to unemployment or health issues. These associations were of similar magnitude in men and women. When current asthma was used, the associations were more pronounced in women for some career path indicators.
Adverse Metabolic Effects on Glucose in Patients Receiving Anamorelin Using a Japanese Claims Database
Authors: Ohta Hiroaki , Horii Takeshi , Yasu Takeo
Published: August 2023

Background: Anamorelin is the first drug approved for the treatment of cancer cachexia, a debilitating condition characterized by weight loss, anorexia, and muscle mass depletion. Cachexia negatively affects a patient’s quality of life, survival, and response to chemotherapy. Studies describing anamorelin use are currently limited to a small number of pancreatic cancer cases. Objectives: We aimed to examine the incidence and risk factors of adverse metabolic effects on glucose levels in cachexia patients with various carcinomas treated with anamorelin. Method: We used real-world data of patients who received anamorelin between August 2021 and July 2022 and were registered in the JMDC claims database. We investigated the impact of metabolic adverse effects on glucose in patients receiving anamorelin with respect to the following factors: sex (male), age (>75 years), types of carcinoma, history of diabetes mellitus (DM), and concomitant use of steroids. Results: The incidence of adverse metabolic effects on glucose was 12.3%, and pancreatic cancer and history of DM were associated with adverse metabolic effects on glucose. The median onset of adverse metabolic effects on glucose was 17 days after anamorelin treatment. Conclusions: This study highlights the need to monitor and manage hyperglycemia in cachexia patients receiving anamorelin, especially in those with pancreatic cancer and a history of DM.