bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Effects of Neuromuscular- and Strength-Training Protocols on Pathomechanical, Sensory-Perceptual, and Motor-Behavioral Impairments in Patients with Chronic Ankle Instability: Randomized Controlled Trial
Authors: Kim Kyung-Min , Estepa-Gallego Alejandro , Estudillo-Martínez María D. , Castellote-Caballero Yolanda , Cruz-Díaz David
Published: July 2022

(1) Background: Chronic ankle instability (CAI) is a complex condition that includes limited mobility, perceived instability, and recurrent ankle sprains are common characteristics that reduce the quality of life in subjects who suffer from CAI. Neuromuscular training and strength training have been recommended in CAI management interventions. However, there are contradictory findings on results when comparing neuromuscular training, strength training, and the control group. The objective of this study was to compare the effectiveness of 8 weeks of neuromuscular intervention training, strength training, and no intervention in a sporting population with reported CAI. (2) Methods: Sixty-seven athletes with CAI were randomly assigned to a neuromuscular training group (NG), strength training group (SG), or control group (CG). Participants completed 8 weeks of neuromuscular training (a combination of static and dynamic exercises), strength training (resistance band exercises), or no training. Outcome measures were assessed at baseline and after 8 weeks and included selfs-reported instability feeling (CAIT), dynamic balance (SEBT), ankle dorsiflexion range of motion (WBLT), and functional status (FAAM and FAAM-SPORT). (3) Results: There were significant differences between strength and control groups in the posteromedial direction of SEBT, FAAM, and FAAM-SPORT after 8 weeks of intervention. (4) Conclusions: Neuromuscular training and strength training based on resistance bands exercises showed significant improvements in ankle dorsiflexion, subjective feeling of instability, functional status, and dynamic balance in patients with CAI.
Acute Hyperhemolysis Syndrome in a Patient with Known Sickle Cell Anemia Refractory to Steroids and IVIG Treated with Tocilizumab and Erythropoietin: A Case Report and Review of Literature
Authors: Menakuru Sasmith R. , Priscu Adelina , Dhillon Vijaypal , Salih Ahmed
Published: July 2022

Patients with sickle cell anemia often receive multiple red blood cell (RBC) transfusions during their lifetime. Hyperhemolysis is a life-threatening phenomenon of accelerated hemolysis and worsening anemia that occurs when both transfused RBCs and autologous RBCs are destroyed. The level of hemoglobin post-transfusion is lower than pre-transfusion levels, and patients are usually hemodynamically unstable. Hyperhemolysis must be differentiated from a delayed hemolytic transfusion reaction during which destruction of transfused RBC is the cause of anemia. Hyperhemolysis syndrome can be differentiated into acute (within seven days) and chronic forms (after seven days) post-transfusion. The authors present a case of acute hyperhemolysis syndrome in a patient with sickle cell anemia refractory to steroids and IVIG, which are the treatment of choice. The patient was treated with tocilizumab, combined with supportive measures of erythropoietin, iron, vitamin B12, and folate.
The Emotional Experience of Flowers: Zoomed In, Zoomed Out and Painted
Authors: Urakami Jacqueline , Huss Ephrat , Nagamine Mitsue , Czamanski-Cohen Johanna , Zaccai Michele
Published: July 2022

People have an ancient and strong bond to flowers, which are known to have a positive effect on the mood. During the COVID-19 pandemic, sales of ornamental plants increased, and many turned to gardening, possibly as a way to cope with ubiquitous increases in negative mood following lockdowns and social isolation. The nature of the special bond between humans and flowers requires additional elucidation. To this means, we conducted a comprehensive online mixed methods study, surveying 253 individuals (ages 18–83) from diverse ethnic backgrounds and continents, regarding their thoughts and feelings towards photos of flowers, nature scenes and flower drawings. We found that looking at pictures and drawings of flowers, as well as nature scenes induced positive emotions, and participants reported a variety of positive responses to the images. More specifically, we found associations of flowers with femininity, and connotations to particular flowers that were affected by geographical location. While nature scene photos induced positive reactions, flower photos were preferred, denying a mere substitution of nature by flowers and vice versa. Drawings of flowers elicited less positive emotions than photos, as people related more to the art than to the flower itself. Our study reveals the importance of ornamental flowers and nature in our life and well-being, and as such their cultivation and promotion are essential.
Methods to Improve the First Hydrogenation of the Vanadium-Rich BCC Alloy Ti16V60Cr24
Authors: Ravalison Francia , Rabkin Eugen , Huot Jacques
Published: July 2022

In this paper we report the effect of three different preparation methods on the first hydrogenation of the vanadium-rich BCC alloy Ti16V60Cr24: one-time cold rolling, 5 min ball milling and addition of 4 wt.% of Zr. All samples were synthesized by arc melting. Without Zr addition the alloy was single phase, but when 4 wt.% Zr was added, a secondary zirconium-rich phase was present. However, X-ray diffraction patterns only showed a single-body-centred cubic phase before hydrogenation for all samples. The crystal structure of the fully hydrogenated samples was body-centred tetragonal. The highest hydrogen capacity (3.8 wt.%) was measured for the Zr-doped alloy. The ball-milled alloy also exhibited a high storage capacity and fast kinetics. However, the maximum hydrogen storage capacity slightly decreased after cold rolling. It was found that air exposure increases incubation time for the first hydrogenation. The incubation time was shortened by cold rolling which, however, reduced the hydrogen storage capacity. The Pressure-Composition isotherms of Ti16V60Cr24 + 4 wt.% Zr at 297, 303 and 323 K were determined. The determined enthalpy and entropy of hydrides formation were −41 ± 5 kJ/mol and −134 ± 14 J/mol/K, respectively.
Spatiotemporal Characteristics and Influencing Factors of Tourism–Urbanization–Technology–Ecological Environment on the Yunnan–Guizhou–Sichuan Region: An Uncoordinated Coupling Perspective
Authors: Yang Guangming , Gong Guofang , Luo Yao , Yang Yunrui , Gui Qingqing
Published: July 2022

The tourism, urbanization, technology, and the ecological environment both promote and restrict each other. Coordinating the relationship between the four is of great significance to the realization of high-quality sustainable regional development. Taking the Yunnan–Guizhou–Sichuan region as an example, this paper constructs an uncoordinated coupling model for the tourism–urbanization–technology–ecological environment system. Using exploratory spatial analysis and geographic information systems, this paper reveals the temporal and spatial evolution law affecting the uncoordinated coupling relationship between tourism, urbanization, technology and the ecological environment in the Yunnan–Guizhou–Sichuan region from 2010 to 2020, before establishing a panel Tobit model that is used to explore the factors affecting the four systems. The research shows the following: (1) The level of comprehensive development for tourism, urbanization, technology, and the ecological environment in Yunnan, Guizhou, and Sichuan has increased rapidly. Of all these, the tourism industry was the most affected by COVID-19 in 2020, while the level of urbanization, technology, and ecological environment developments in the three provinces has become similar over time. (2) Uncoordinated development between cities is a prominent problem; while the uncoordinated coupling spatial agglomeration in various regions is relatively stable, the proportion of cities with no significant agglomeration form amounts to more than 70%, with mostly low–low (L–L) and high–high (H–H) agglomeration types. (3) The degree to which uncoordinated coupling exists among the four systems in the Yunnan–Guizhou–Sichuan region is affected by many factors. Only eco-environmental pressure has a significant positive correlation with the degree of uncoordinated coupling, while the tourism scale, economic urbanization, eco-environmental response, and investment in technology have a significant negative correlation. These results provide a theoretical basis and practical references for strengthening the government’s macro-control and promoting collaborative regional development.
Parental Distress and Affective Perception of Hospital Environment after a Pictorial Intervention in a Neonatal Intensive Care Unit
Authors: Neri Erica , Genova Federica , Stella Marcello , Provera Alessandra , Biasini Augusto , Agostini Francesca
Published: July 2022

Pictorial humanization is a useful intervention for the improvement of hospitalized patients’ affective states. Despite benefits in many hospital wards having been well documented, so far, no attention was paid to the Neonatal Intensive Care Unit (NICU). The aim of the present study was to evaluate the levels of distress and the affective perception of the environment experienced by parents of infants hospitalized in a NICU after the implementation of an intervention of pictorial humanization. A sample of 48 parents was recruited, 25 before the intervention was performed (Control Group), and 23 after its implementation (Pictorial Humanization Group). All parents completed the “Rapid Stress Assessment Scale” and “Scales of the Affective Quality Attributed to Place” questionnaires. Despite results showing no significant differences on parental distress, after implementation of pictorial intervention parents reported a perception of the NICU as significantly more pleasant, exciting, and arousing, and less distressing, unpleasant, gloomy, and sleepy. A higher level of distress and a perception of the environment as less relaxing was predicted for the Control Group condition. The present study suggests that the pictorial intervention represents a useful technique to create more welcoming hospital environments and to reduce the negative effects associated with infant hospitalization.
Thermography as a Non-Ionizing Quantitative Tool for Diagnosing Burning Mouth Syndrome: Case-Control Study
Authors: Nicolas-Rodriguez Elena , Garcia-Martinez Ana , Molino-Pagan Diana , Marin-Martinez Luis , Pons-Fuster Eduardo , López-Jornet Pia
Published: July 2022

Objectives: Thermography is an imaging technique based on the acquisition and analysis of thermal data. The present study evaluates the use of tongue infrared thermography (IRT) as a tool for the diagnosis of burning mouth syndrome (BMS). Material and methods: An IRT study was carried out in patients diagnosed with BMS according to the criteria of the International Association for the Study of Pain (n = 32) and in healthy controls (n = 35). Burning sensations, dry mouth and taste disturbances were assessed, and three temperature values were recorded for each tongue surface (dorsal, right lateral, left lateral and tip), along with body temperature and environmental temperature. Results: A statistically significant difference was recorded in the temperature of the dorsal surface of the tongue between the BMS group and the controls (p = 0.01). The area under the curve (AUC) was 0.731 (95% CI: 0.402–0.657; p = 0.003). The sensitivity and specificity obtained was 62% and 77%, respectively. Conclusions: Infrared thermography appears to be useful as a complementary tool for the diagnosis of BMS, though further studies are needed in this field.
Herbal Medicine Uses for Respiratory System Disorders and Possible Trends in New Herbal Medicinal Recipes during COVID-19 in Pasvalys District, Lithuania
Authors: Pranskuniene Zivile , Balciunaite Ruta , Simaitiene Zenona , Bernatoniene Jurga
Published: July 2022

Despite some preliminary studies of the available herbal medicine preparations and their curative effects on COVID-19, experts still fear that unproper use of such homemade medicines could do more harm than good to people relying on unproven alternatives of questionable efficacy. The main purpose of this study was to evaluate the safety of herbal medicines used for respiratory system disorders in the Pasvalys district during the COVID-19 pandemic in Lithuania. An archival source was also studied, looking for possible recipes for the treatment and prevention of respiratory diseases in Lithuanian traditional medicine, emphasizing the safety guidelines. The survey was conducted using the deep interview method. The respondents mentioned 60 species of medicinal plants from 29 different families used for the treatment and prevention of respiratory system disorders (for cough mostly, 51.70% of all indications). Twenty eight out of 60 plant species were not included in the European Medicines Agency monographs and only 50% of all included species were used as indicated by the European Medicines Agency for respiratory system disorders. The trends in the ethnopharmacological choices of modern consumers and the analysis of archival sources can be a great source of ideas for new herbal-based pharmaceutical preparations for COVID-19 symptoms in Lithuania considering the safety recommendations.
A Comparative Study of the Resilience of Urban and Rural Areas under Climate Change
Authors: Su Qingmu , Chang Hsueh-Sheng , Pai Shin-En
Published: July 2022

The impact of climate change in recent years has caused considerable risks to both urban and rural systems. How to mitigate the damage caused by extreme weather events has attracted much attention from countries in recent years. However, most of the previous studies on resilience focused on either urban areas or rural areas, and failed to clearly identify the difference between urban and rural resilience. In fact, the exploration of the difference between the resilience characteristics of cities and villages under climate change can help to improve the planning strategy and the allocation of resources. In this study, the indicators of resilience were firstly built through a literature review, and then a Principal Component Analysis was conducted to construct an evaluation system involving indicators such as “greenland resilience”, “community age structure resilience”, “traditional knowledge resilience”, “infrastructure resilience” and “residents economic independence resilience”. Then the analysis of Local Indicators of Spatial Association showed some resilience abilities are concentrated in either urban or rural. Binary logistic regression was performed, and the results showed urban areas have more prominent abilities in infrastructure resilience (the coefficient value is 1.339), community age structure resilience (0.694), and greenland resilience (0.3), while rural areas are more prominent in terms of the residents economic independence resilience (−0.398) and traditional knowledge resilience (−0.422). It can be seen that urban areas rely more on the resilience of the socio-economic structure, while rural areas are more dependent on their own knowledge and economic independence. This result can be used as a reference for developing strategies to improve urban and rural resilience.
Myopia and Other Visual Disorders in Children
Authors: Alvarez-Peregrina Cristina , Martinez-Perez Clara , Sánchez-Tena Miguel Ángel
Published: July 2022

Ocular pathologies can lead to permanent vision loss and pose an important public health problem [...]
Desire for Genital Surgery in Trans Masculine Individuals: The Role of Internalized Transphobia, Transnormativity and Trans Positive Identity
Authors: Anzani Annalisa , Biella Marco , Scandurra Cristiano , Prunas Antonio
Published: July 2022

Some trans people experience gender dysphoria, which refers to psychological distress that results from an incongruence between one’s gender assigned at birth and one’s gender identity. People who are trans masculine or nonbinary assigned-female-at-birth may pursue multiple domains of gender affirmation, including surgical affirmation (e.g., masculine chest reconstruction, penile reconstruction, etc.). The present study aimed to investigate the possible factors involved in trans people’s desire to undergo gender-affirming genital surgery. Trans masculine and nonbinary participants (N = 127; mean age = 26.90) were recruited through a web-based survey and completed self-report instruments (i.e., the Internalized Transphobia subscale of the Gender Minority Stress and Resilience Measure, the Trans Positive Identity Measure, the Gender Congruence and Life Satisfaction Scale, an ad hoc scale on transnormativity, and a single-item on desire to undergo genital affirmation surgery). A path analysis showed that higher levels of internalized transphobia led to more significant genital discomfort via a dual parallel mediation of transnormativity and positive identity. Moreover, this genital discomfort fueling pattern was the most significant predictor of the desire to undergo genital surgery as the effect of internalized transphobia was fully mediated by increased genital discomfort. Findings are discussed in the light of the recent strand of research on gender dysphoria as a multifaceted construct, with social components.
Spatial Syndromic Surveillance and COVID-19 in the U.S.: Local Cluster Mapping for Pandemic Preparedness
Authors: Curtis Andrew , Ajayakumar Jayakrishnan , Curtis Jacqueline , Brown Sam
Published: July 2022

Maps have become the de facto primary mode of visualizing the COVID-19 pandemic, from identifying local disease and vaccination patterns to understanding global trends. In addition to their widespread utilization for public communication, there have been a variety of advances in spatial methods created for localized operational needs. While broader dissemination of this more granular work is not commonplace due to the protections under Health Insurance Portability and Accountability Act (HIPAA), its role has been foundational to pandemic response for health systems, hospitals, and government agencies. In contrast to the retrospective views provided by the aggregated geographies found in the public domain, or those often utilized for academic research, operational response requires near real-time mapping based on continuously flowing address level data. This paper describes the opportunities and challenges presented in emergent disease mapping using dynamic patient data in the response to COVID-19 for northeast Ohio for the period 2020 to 2022. More specifically it shows how a new clustering tool developed by geographers in the initial phases of the pandemic to handle operational mapping continues to evolve with shifting pandemic needs, including new variant surges, vaccine targeting, and most recently, testing data shortfalls. This paper also demonstrates how the geographic approach applied provides the framework needed for future pandemic preparedness.
Incidence and Predictors of Mortality among Community-Dwelling Older Adults in Malaysia: A 5 Years Longitudinal Study
Authors: You Yee Xing , Rivan Nurul Fatin Malek , Singh Devinder Kaur Ajit , Rajab Nor Fadilah , Ludin Arimi Fitri Mat , Din Normah Che , Chin Ai-Vyrn , Fenech Michael , Kamaruddin Mohd Zul Amin , Shahar Suzana
Published: July 2022

With older adults accounting for 10.7% of the Malaysian population, determining the predictors of mortality has now become crucial. Thus, this community-based longitudinal study aimed to investigate the predictors for mortality among community-dwelling older adults using a wide range of factors, including clinical or subclinical. A total of 2322 older adults were interviewed and assessed by trained fieldworkers using validated structured questionnaires. The questionnaire consisted of information on socio-demographic characteristics, health status, neuropsychological and psychosocial functions, lifestyle, dietary intake and biophysical measures. The incidence rate of mortality was 2.9 per 100 person-years. Cox regression analysis indicated that advancing age (Adjusted Hazard Ratio, Adj HR = 1.044, 95% CI: 1.024–1.064), male (Adj HR = 1.937, 95% CI: 1.402–2.675), non-married status (Adj HR = 1.410, 95% CI: 1.078–1.843), smoking (Adj HR = 1.314, 95% CI: 1.004–1.721), a higher fasting blood sugar (Adj HR = 1.075, 95% CI: 1.029–1.166), a lower serum albumin (Adj HR = 0.947, 95% CI: 0.905–0.990), a longer time to complete the TUG test (Adj HR = 1.059, 95% CI: 1.022–1.098), and a lower intake of total dietary fibre (Adj HR = 0.911, 95% CI: 0.873–0.980) were the predictors of mortality in this study. These findings provide an estimated rate of multiethnic mortality in middle-income countries and diet is one of the predictors. These predictors of mortality could be a reference in identifying new public health strategies to ensure longer healthier life spans with lower disability rate among community-dwelling older adults in Malaysia.
Relationship between Income and Mental Health during the COVID-19 Pandemic in China
Authors: Li Mingna , Zhou Bo , Hu Bingbin
Published: July 2022

Mental health problems represent one most pressing concerns in the world, which produce costly consequences for individuals, families and society as a whole. One of the determinants on mental health during the COVID-19 pandemic is income. To complement relevant research and provide valuable recommendations for governments and societies, this study investigates the nexus between income and mental health in China by employing 8049 observations from the 2020 China Family Panel Studies. Using ordinary least squares regression we find the significant positive relationship between income and mental health, and estimate the effect of income on mental health. Furthermore, this effect is heterogeneous depending on individuals’ education level and registered residence type. Finally, individuals’ economic status and happiness are shown to be the potential mechanism through which the effect of income on mental health operates.
Interactive Visualization and Representation Analysis Applied to Glacier Segmentation
Authors: Zheng Minxing , Miao Xinran , Sankaran Kris
Published: July 2022

Interpretability has attracted increasing attention in earth observation problems. We apply interactive visualization and representation analysis to guide the interpretation of glacier segmentation models. We visualize the activations from a U-Net to understand and evaluate the model performance. We built an online interface using the Shiny R package to provide comprehensive error analysis of the predictions. Users can interact with the panels and discover model failure modes. We illustrate an example of how our interface could help guide decisions for improving model performance. Further, we discuss how visualization can provide sanity checks during data preprocessing and model training. By closely examining the problem of glacier segmentation, we are able to discuss how visualization strategies can support the modeling process and the interpretation of prediction results from geospatial deep learning.
Identifying Suitable Watersheds across Nigeria Using Biophysical Parameters and Machine Learning Algorithms for Agri–Planning
Authors: Panjala Pranay , Gumma Murali Krishna , Ajeigbe Hakeem Ayinde , Badamasi Murtala Muhammad , Deevi Kumara Charyulu , Tabo Ramadjita
Published: July 2022

Identifying suitable watersheds is a prerequisite to operationalizing planning interventions for agricultural development. With the help of geospatial tools, this paper identified suitable watersheds across Nigeria using biophysical parameters to aid agricultural planning. Our study included various critical thematic layers such as precipitation, temperature, slope, land-use/land-cover (LULC), soil texture, soil depth, and length of growing period, prepared and modeled on the Google Earth Engine (GEE) platform. Using expert knowledge, scores were assigned to these thematic layers, and a priority map was prepared based on the combined weighted average score. We also validated priority watersheds. For this, the study area was classified into three priority zones ranging from ‘high’ to ‘low’. Of the 277 watersheds identified, 57 fell in the high priority category, implying that they are highly favorable for interventions. This would be useful for regional-scale water resource planning for agricultural landscape development.
Size-Dependent Spontaneous Separation of Colloidal Particles in Sub-Microliter Suspension by Cations
Authors: Sheng Shiqi , Yang Haijun , Song Yongshun , Chen Ruoyang , Liang Shanshan , Fang Haiping
Published: July 2022

Great efforts have been made to separate micro/nanoparticles in small-volume specimens, but it is a challenge to achieve the simple, maneuverable and low-cost separation of sub-microliter suspension with large separation distances. By simply adding trace amounts of cations (Mg2+/Ca2+/Na+), we experimentally achieved the size-dependent spontaneous separation of colloidal particles in an evaporating droplet with a volume down to 0.2 μL. The separation distance was at a millimeter level, benefiting the subsequent processing of the specimen. Within only three separating cycles, the mass ratio between particles with diameters of 1.0 μm and 0.1 μm can be effectively increased to 13 times of its initial value. A theoretical analysis indicates that this spontaneous separation is attributed to the size-dependent adsorption between the colloidal particles and the aromatic substrate due to the strong hydrated cation-π interactions.
Genome-Wide Characterization and Phylogenetic Analysis of GSK Genes in Maize and Elucidation of Their General Role in Interaction with BZR1
Authors: Li Hui , Luo Li , Wang Yayun , Zhang Junjie , Huang Yubi
Published: July 2022

Glycogen synthase kinase-3 (GSK-3) is a nonreceptor serine/threonine protein kinase that is involved in diverse processes, including cell development, photomorphogenesis, biotic and abiotic stress responses, and hormone signaling. In contrast with the deeply researched GSK family in Arabidopsis and rice, maize GSKs’ common bioinformatic features and protein functions are poorly understood. In this study, we identified 11 GSK genes in the maize (Zea mays L.) genome via homologous alignment, which we named Zeama;GSKs (ZmGSKs). The results of ZmGSK protein sequences, conserved motifs, and gene structures showed high similarities with each other. The phylogenetic analyses showed that a total of 11 genes from maize were divided into four clades. Furthermore, semi-quantitative RT-PCR analysis of the GSKs genes showed that ZmGSK1, ZmGSK2, ZmGSK4, ZmGSK5, ZmGSK8, ZmGSK9, ZmGSK10, and ZmGSK11 were expressed in all tissues; ZmGSK3, ZmGSK6, and ZmGSK7 were expressed in a specific organization. In addition, GSK expression profiles under hormone treatments demonstrated that the ZmGSK genes were induced under BR conditions, except for ZmGSK2 and ZmGSK5. ZmGSK genes were regulated under ABA conditions, except for ZmGSK1 and ZmGSK8. Finally, using the yeast two-hybrid and BiFC assay, we determined that clads II (ZmGSK1, ZmGSK4, ZmGSK7, ZmGSK8, and ZmGSK11) could interact with ZmBZR1. The results suggest that clade II of ZmGSKs is important for BR signaling and that ZmGSK1 may play a dominant role in BR signaling as the counterpart to BIN2. This study provides a foundation for the further study of GSK3 functions and could be helpful in devising strategies for improving maize.
Boron Derivatives Accelerate Biofilm Formation of Recombinant Escherichia coli via Increasing Quorum Sensing System Autoinducer-2 Activity
Authors: Chen Huan , Yan Cheng-Hai , Zhan Yu-Fan , Geng Li-Tian , Zhu Lin-Lin , Gong Lu-Chan , Wang Jun
Published: July 2022

Boron is an essential element for autoinducer-2 (AI-2) synthesis of quorum sensing (QS) system, which affects bacterial collective behavior. As a living biocatalyst, biofilms can stably catalyze the activity of intracellular enzymes. However, it is unclear how boron affects biofilm formation in E. coli, particularly recombinant E. coli with intracellular enzymes. This study screened different boron derivatives to explore their effect on biofilm formation. The stress response of biofilm formation to boron was illuminated by analyzing AI-2 activity, extracellular polymeric substances (EPS) composition, gene expression levels, etc. Results showed that boron derivatives promote AI-2 activity in QS system. After treatment with H3BO3 (0.6 mM), the AI-2 activity increased by 65.99%, while boron derivatives increased the biomass biofilms in the order H3BO3 > NaBO2 > Na2B4O7 > NaBO3. Moreover, treatment with H3BO3 (0.6 mM) increased biomass by 88.54%. Meanwhile, AI-2 activity had a linear correlation with polysaccharides and protein of EPS at 0–0.6 mM H3BO3 and NaBO2 (R2 > 0.8). Furthermore, H3BO3 upregulated the expression levels of biofilm formation genes, quorum sensing genes, and flagellar movement genes. These findings demonstrated that boron promoted biofilm formation by upregulating the expression levels of biofilm-related genes, improving the QS system AI-2 activity, and increasing EPS secretion in E. coli.
mTOR Modulation of IKr through hERG1b-Dependent Mechanisms in Lipotoxic Heart
Authors: Aromolaran Kelly A. , Do Jenny , Bernardi Joyce , Aromolaran Ademuyiwa S.
Published: July 2022

In the atria, the rapid delayed rectifier channel (IKr) is a critical contributor to repolarization. In lipotoxic atria, increased activity of the serine/threonine mammalian target of rapamycin (mTOR) may remodel IKr and predispose patients to arrhythmias. To investigate whether mTOR produced defects in IKr channel function (protein expression and gating mechanisms), electrophysiology and biochemical assays in HEK293 cells stably expressing hERG1a/1b, and adult guinea pig atrial myocytes were used. Feeding with the saturated fatty acid palmitic acid high-fat diet (HFD) was used to induce lipotoxicity. Lipotoxicity-challenged HEK293 cells displayed an increased density of hERG1a/1b currents due to a targeted and significant increase in hERG1b protein expression. Furthermore, lipotoxicity significantly slowed the hERG1a/1b inactivation kinetics, while the activation and deactivation remained essentially unchanged. mTOR complex 1 (mTORC1) inhibition with rapamycin (RAP) reversed the increase in hERG1a/1b density and inactivation. Compared to lipotoxic myocytes, RAP-treated cells displayed action potential durations (APDs) and IKr densities similar to those of controls. HFD feeding triggered arrhythmogenic changes (increased the IKr density and shortened the APD) in the atria, but this was not observed in low-fat-fed controls. The data are the first to show the modulation of IKr by mTORC1, possibly through the remodeling of hERG1b, in lipotoxic atrial myocytes. These results offer mechanistic insights with implications for targeted therapeutic options for the therapy of acquired supraventricular arrhythmias in obesity and associated pathologies.
Uterine Fibroids Causing Preterm Birth: A New Pathophysiological Hypothesis on the Role of Fibroid Necrosis and Inflammation
Authors: Don Emma E. , Landman Anadeijda J. E. M. C. , Vissers Guus , Jordanova Ekaterina S. , Post Uiterweer Emiel D. , de Groot Christianne J. M. , de Boer Marjon A. , Huirne Judith A. F.
Published: July 2022

According to recent studies and observations in clinical practice, uterine fibroids increase the risk of preterm birth. There are several theories on the pathogenesis of preterm birth in the presence of fibroids. One theory proclaims that fibroid necrosis leads to preterm birth, though pathophysiological mechanisms have not been described. Necrotic tissue secretes specific cytokines and proteins and we suggest these to be comparable to the inflammatory response leading to spontaneous preterm birth. We hypothesize that fibroid necrosis could induce preterm parturition through a similar inflammatory response. This new hypothesis generates novel perspectives for future research and the development of preventative strategies for preterm birth. Moreover, we emphasize the importance of the recognition of fibroids and especially fibroid necrosis by clinicians during pregnancy.
Piezo1 Channel as a Potential Target for Hindering Cardiac Fibrotic Remodeling
Authors: Braidotti Nicoletta , Chen Suet Nee , Long Carlin S. , Cojoc Dan , Sbaizero Orfeo
Published: July 2022

Fibrotic tissues share many common features with neoplasms where there is an increased stiffness of the extracellular matrix (ECM). In this review, we present recent discoveries related to the role of the mechanosensitive ion channel Piezo1 in several diseases, especially in regulating tumor progression, and how this can be compared with cardiac mechanobiology. Based on recent findings, Piezo1 could be upregulated in cardiac fibroblasts as a consequence of the mechanical stress and pro-inflammatory stimuli that occurs after myocardial injury, and its increased activity could be responsible for a positive feedback loop that leads to fibrosis progression. The increased Piezo1-mediated calcium flow may play an important role in cytoskeleton reorganization since it induces actin stress fibers formation, a well-known characteristic of fibroblast transdifferentiation into the activated myofibroblast. Moreover, Piezo1 activity stimulates ECM and cytokines production, which in turn promotes the phenoconversion of adjacent fibroblasts into new myofibroblasts, enhancing the invasive character. Thus, by assuming the Piezo1 involvement in the activation of intrinsic fibroblasts, recruitment of new myofibroblasts, and uncontrolled excessive ECM production, a new approach to blocking the fibrotic progression can be predicted. Therefore, targeted therapies against Piezo1 could also be beneficial for cardiac fibrosis.
Lipoprotein Profile in Immunological Non-Responders PLHIV after Antiretroviral Therapy Initiation
Authors: Masip Jenifer , Jorba Rosa , López-Dupla Miguel , Domingo Pere , Pacheco Yolanda María , García-Pardo Graciano , Martínez Esteban , Viladés Consuelo , Veloso Sergi , Alba Verónica , Olona Montserrat , Vidal Francesc , Gómez-Bertomeu Frederic , Peraire Joaquim , Rull Anna
Published: July 2022

Nuclear magnetic resonance (NMR)-based advanced lipoprotein tests have demonstrated that LDL and HDL particle numbers (LDL-P and HDL-P) are more powerful cardiovascular (CV) risk biomarkers than conventional cholesterol markers. Of interest, in people living with HIV (PLHIV), predictors of preclinical atherosclerosis and vascular dysfunction may be associated with impaired immune function. We previously stated that immunological non-responders (INR) were at higher CV risk than immunological responders (IR) before starting antiretroviral therapy (ART). Using Liposcale® tests, we characterized the lipoprotein profile from the same cohort of PLHIV at month 12 and month 36 after starting ART, intending to explore what happened with these indicators of CV risk during viral suppression. ART initiation dissipates the differences in lipoprotein-based CV risk markers between INR and IR, and only an increase in the number of HDL-P was found in INR + IR when compared to controls (p = 0.047). Interestingly, CD4+ T-cell counts negatively correlated with medium HDL-P concentrations at month 12 in all individuals (ρ = −0.335, p = 0.003). Longitudinal analyses showed an important increase in LDL-P and HDL-P at month 36 when compared to baseline values in both IR and INR. A proper balance between a proatherogenic and atherogenic environment may be related to the reconstitution of CD4+ T-cell count in PLHIV.
Cardiac Fibrosis: Chronic Inflammatory Disease and Promising Therapeutic Target
Authors: Okamoto Ryuji
Published: July 2022

A better understanding on the cause, pathophysiologic mechanisms and potential new treatments of cardiac fibrosis is one of the main issues in the management of cardiac hypertrophy, heart failure and lethal arrhythmia [...]
Analysis of the Biological Properties of Blood Plasma Protein with GcMAF Functional Activity
Authors: Dolgova Evgeniya V. , Kirikovich Svetlana S. , Levites Evgeniy V. , Ruzanova Vera S. , Proskurina Anastasia S. , Ritter Genrikh S. , Taranov Oleg S. , Varaksin Nikolay A. , Ryabicheva Tatiana G. , Leplina Olga Yu. , Ostanin Alexandr A. , Chernykh Elena R. , Bogachev Sergey S.
Published: July 2022

The main problem related to the studies focusing on group-specific component protein-derived macrophage-activating factor (GcMAF) is the lack of clarity about changes occurring in different types of macrophages and related changes in their properties under the effect of GcMAF in various clinical conditions. We analyzed the antitumor therapeutic properties of GcMAF in a Lewis carcinoma model in two clinical conditions: untreated tumor lesion and tumor resorption after exposure to Karanahan therapy. GcMAF is formed during site-specific deglycosylation of vitamin D3 binding protein (DBP). DBP was obtained from the blood of healthy donors using affinity chromatography on a column with covalently bound actin. GcMAF-related factor (GcMAF-RF) was converted in a mixture with induced lymphocytes through the cellular enzymatic pathway. The obtained GcMAF-RF activates murine peritoneal macrophages (p < 0.05), induces functional properties of dendritic cells (p < 0.05) and promotes in vitro polarization of human M0 macrophages to M1 macrophages (p < 0.01). Treatment of whole blood cells with GcMAF-RF results in active production of both pro- and anti-inflammatory cytokines. It is shown that macrophage activation by GcMAF-RF is inhibited by tumor-secreted factors. In order to identify the specific antitumor effect of GcMAF-RF-activated macrophages, an approach to primary reduction of humoral suppressor activity of the tumor using the Karanahan therapy followed by macrophage activation in the tumor-associated stroma (TAS) was proposed. A prominent additive effect of GcMAF-RF, which enhances the primary immune response activation by the Karanahan therapy, was shown in the model of murine Lewis carcinoma. Inhibition of the suppressive effect of TAS is the main condition required for the manifestation of the antitumor effect of GcMAF-RF. When properly applied in combination with any chemotherapy, significantly reducing the humoral immune response at the advanced tumor site, GcMAF-RF is a promising antitumor therapeutic agent that additively destroys the pro-tumor properties of macrophages of the tumor stroma.
Modeling of Proteolysis of β-Lactoglobulin and β-Casein by Trypsin with Consideration of Secondary Masking of Intermediate Polypeptides
Authors: Vorob’ev Mikhail M.
Published: July 2022

The opening of protein substrates during degradation by proteases and the corresponding exposure of their internal peptide bonds for a successful enzymatic attack, the so-called demasking effect, was studied for β-lactoglobulin (β-LG) and β-casein (β-CN) hydrolyzed by trypsin. Demasking was estimated by monitoring the redshift in intrinsic tryptophan fluorescence, characterizing the accessibility of polypeptide chains to aqueous medium. The secondary masking of intermediate polypeptides, giving an inverse effect to demasking, caused a restriction of the substrate opening. This led to the limitations in the red shift of fluorescence and the degree of hydrolysis with a long time of hydrolysis of β-LG and β-CN at a constant substrate concentration and reduced trypsin concentrations. The proposed proteolysis model included demasking of initially masked bonds in the protein globule or micelle, secondary masking of intermediate polypeptides, and their subsequent slow demasking. The hydrolysis of peptide bonds was modeled taking into account different hydrolysis rate constants for different peptide bonds. It was demonstrated that demasking competes with secondary masking, which is less noticeable at high trypsin concentrations. Modeling of proteolysis taking into account two demasking processes and secondary masking made it possible to simulate kinetic curves consistent with the experimental data.
Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral Ischemia
Authors: Faura Júlia , Ramiro Laura , Simats Alba , Ma Feifei , Penalba Anna , Gasull Teresa , Rosell Anna , Montaner Joan , Bustamante Alejandro
Published: July 2022

After stroke and other brain injuries, there is a high incidence of respiratory complications such as pneumonia or acute lung injury. The molecular mechanisms that drive the brain-lung interaction post-stroke have not yet been elucidated. We performed transient middle cerebral artery occlusion (MCAO) and sham surgery on C57BL/6J mice and collected bronchoalveolar lavage fluid (BALF), serum, brain, and lung homogenate samples 24 h after surgery. A 92 proteins-panel developed by Olink Proteomics® was used to analyze the content in BALF and lung homogenates. MCAO animals had higher protein concentration levels in BALF than sham-controls, but these levels did not correlate with the infarct volume. No alteration in alveolar-capillary barrier permeability was observed. A total of 12 and 14 proteins were differentially expressed between the groups (FDR < 0.1) in BALF and lung tissue homogenates, respectively. Of those, HGF, TGF-α, and CCL2 were identified as the most relevant to this study. Their protein expression patterns were verified by ELISA. This study confirmed that post-stroke lung damage was not associated with increased lung permeability or cerebral ischemia severity. Furthermore, the dysregulation of HGF, TGF-α, and CCL2 in BALF and lung tissue after ischemia could play an important role in the molecular mechanisms underlying stroke-induced lung damage.
The Deinstitutionalization of Business Support Functions through Artificial Intelligence
Authors: Bauer Jan Christian , Wolff Michael
Published: July 2022

Technological advances in the field of artificial intelligence offer enormous potential for organizations. In recent years, organizations have leveraged this potential by establishing new business models or adjusting their primary activities. In the meantime, however, the potential for greater efficiency and effectiveness in support functions such as human resource management (HRM), supply chain management (SCM), or financial management (FM) through these technological advances is also increasingly being recognized. We synthesize the current state of research on AI regarding the potentials and diffusion within these support functions. Building upon this, we assess the deinstitutionalization power of AI for altering organizational processes within business support functions and derive implications to harness the full potential of AI across organizations.
Performance Evaluation of Blind Modal Identification in Large-Scale Civil Infrastructure
Authors: Abasi Ali , Sadhu Ayan
Published: July 2022

The monitoring and maintenance of existing civil infrastructure has recently received worldwide attention. Several structural health monitoring methods have been developed, including time-, frequency-, and time–frequency domain methods of modal identification and damage detection to estimate the structural and modal parameters of large-scale structures. However, there are several implementation challenges of these modal identification methods, depending on the size of the structures, measurement noise, number of available sensors, and their operational loads. In this paper, two modal identification methods, Second-Order Blind Identification (SOBI) and Time-Varying Filtering Empirical Mode Decomposition (TVF-EMD), are evaluated and compared for large-scale structures including a footbridge and a wind turbine blade with a wide range of dynamic characteristics. The results show that TVF-EMD results in better accuracy in modal identification compared to SOBI for both structures. However, when the number of sensors is equal to or more than the number of target modes of the structure, SOBI results in better computational efficiencies compared to TVF-EMD.
Life Table Parameters and Digestive Enzyme Activity of Spodoptera littoralis (Boisd) (Lepidoptera: Noctuidae) on Selected Legume Cultivars
Authors: Hemmati Seyed Ali , Shishehbor Parviz , Stelinski Lukasz L.
Published: July 2022

Spodoptera littoralis (Boisd) is a highly destructive pest that attacks a large number of economically important crops. We examined life table parameters as well as activity of major digestive enzymes of S. littoralis larvae in response to protein and starch contents across 11 legume cultivars to identify potential resistance traits. The results showed that S. littoralis reared on the common bean, Arabi, displayed the highest intrinsic rate of increase (r), while the lowest was recorded on the cowpea, Mashhad. Also, the highest net reproductive rate (R0) was obtained in those insects reared on the Arabi cultivar. Larvae displayed the highest and lowest proteolytic activities when fed on Mashhad and Arabi cultivars, respectively. The highest amylolytic activity was quantified in larvae that fed on the Arabi and 1057 cultivars, while the lowest occurred in larvae feeding on Yaghout and Mashhad cultivars. Developmental time of S. littoralis larvae was negatively correlated with protein content, while amylolytic activity was positively correlated with starch content of legumes. Our findings indicate that Arabi was a susceptible cultivar, while Mashhad exhibited tolerance traits against S. littoralis. These results should facilitate selection of legume cultivars for production or breeding efforts that involve S. littoralis management.
Sex Pheromone of Cocoa Pod Borer, Conopomorpha cramerella: Field Activity Evaluation of Pheromone Formulations in an Indonesia Plantation
Authors: Niogret Jerome , Ekayanti Arni , Zhang Aijun
Published: July 2022

The previously identified female sex pheromone of cocoa pod borer (CPB), Conopomorpha cramerella (Snellen) (Lepidoptera: Gracillariidae), was re-evaluated for male attraction using six different pheromone formulations in Indonesian cocoa plantations. In a dose-response experiment, the 0.1 mg pheromone load was significantly more attractive to male CPB than the lower doses tested. Additionally, during the first four-week trapping period, USDA (Beltsville, MD, USA) lures containing 0.1 mg of synthetic pheromone blend exhibited significantly better attraction than the commercial lure obtained from Alpha Scents, Inc. (Canby, OR, USA) with the same pheromone load (0.1 mg). Although the 1.0 mg lure did not show any higher attraction than the 0.1 mg lure during the first month, it was significantly attractive for CPB males with the same weekly average capture efficacy for the whole twenty-seven weeks in field conditions in 2018. A long-life pheromone lure can be particularly useful in monitoring large-scale cocoa farms.