bluuebunny/crossref_metadata_2025 huggingface dataset:
Management of Cataract in Patients with Age-Related Macular Degeneration
Authors: Mehta Hemal
Published: June 2021

Cataract and age-related macular degeneration (AMD) are two of the most common eye diseases of aging. This review addresses the pre-operative, intra-operative, and post-operative considerations in managing cataract in patients with age-related macular degeneration. Surgery for visually significant cataracts in patients with AMD can substantially improve the quality of life and reduce the risk of falls. Pre-operative optical coherence tomography is now recommended where possible to identify pre-existing macula disease. Careful counselling of patients is required before cataract surgery, especially with respect to the expected visual outcome, intraocular lens choice and potential risks of surgery. Real-world data has suggested 6 months of intravitreal anti-VEGF therapy for neovascular AMD before cataract surgery is compatible with optimum long-term visual outcomes. Patients receiving intravitreal therapy for neovascular AMD should be advised of the slightly higher risk of intraoperative complications and the surgeon should be prepared to manage these during the operation. During cataract surgery, unnecessary light exposure should be avoided to reduce phototoxicity. Careful planning of intravitreal therapy for neovascular AMD just before cataract surgery allows the eye greater recovery time in the post-operative period before further planned intravitreal therapy.
Infections as Novel Risk Factors of Atherosclerotic Cardiovascular Diseases: Pathophysiological Links and Therapeutic Implications
Authors: Szwed Piotr , Gąsecka Aleksandra , Zawadka Mateusz , Eyileten Ceren , Postuła Marek , Mazurek Tomasz , Szarpak Łukasz , Filipiak Krzysztof J.
Published: June 2021

Atherosclerotic cardiovascular diseases (ASCVD) are the major cause of mortality worldwide. Despite the continuous progress in ASCVD therapy, the residual risk persists beyond the management of traditional risk factors. Several infections including Helicobacter pylori infection, periodontal disease, and viral infections are associated with the increased risk of ASCVD, both directly by damage to the heart muscle and vasculature, and indirectly by triggering a systemic proinflammatory state. Hence, beyond the optimal management of the traditional ASCVD risk factors, infections should be considered as an important non-classical risk factor to enable early diagnosis and appropriate treatment. Here, we summarized the currently available evidence regarding the role of inflammation in ASCVD and the association between the particular infections and pathogens (Helicobacter pylori, periodontal disease, pneumonia, Cytomegalovirus, Human immunodeficiency virus, Herpes simplex virus, and severe acute respiratory syndrome coronavirus 2) on the development and progression of ASCVD. We also speculated about the potential therapeutic implications of the anti-inflammatory and anti-infective drugs on ASCVD outcomes, including drugs routinely administered in patients with ASCVD (statins, P2Y12 receptor inhibitors, and angiotensin-converting enzyme inhibitors/angiotensin receptor blockers) and novel strategies aiming at residual risk reduction (colchicine, anti-cytokine drugs, and methotrexate). Considering the emerging association between infections and ASCVD, it is crucial to determine the possible advantages of infection prevention and treatment in patients with ASCVD.
Immersive Molecular Dynamics in Virtual Reality: Increasing Efficiency of Educational Process with Companion Converter for NarupaXR
Authors: Pereshivkina Polina , Karandasheva Nadezhda , Mikhaylenko Maria , Kurushkin Mikhail
Published: June 2021

Visualization has always been a crucial part of the educational process. Implementing computer algorithms and virtual reality tools into it is vital for the new generation engineers, scientists and researchers. In the field of chemistry education, various software that allow dynamic molecular building and viewing are currently available. These software are now used to enhance the learning process and ensure better understanding of the chemical processes from the visual perspective. The present short communication provides a summary of these applications based on the NarupaXR program, which is a great educational tool that combines the functionality and simple design necessary for an educational tool. NarupaXR is used with a companion application “Narupa Builder” which requires a different file format, therefore a converter that allows a simple transition between the two extensions has been developed. The converter sufficiently increases the efficiency of the educational process. The automatic converter is freely available on GitLab The current communication provides detailed written instructions that can simplify the installation process of the converter and facilitate the use of both the software and the hardware of the VR set.
Soil Reservoir Dynamics of Ophidiomyces ophidiicola, the Causative Agent of Snake Fungal Disease
Authors: Campbell Lewis J. , Burger Joanna , Zappalorti Robert T. , Bunnell John F. , Winzeler Megan E. , Taylor Daniel R. , Lorch Jeffrey M.
Published: June 2021

Wildlife diseases pose an ever-growing threat to global biodiversity. Understanding how wildlife pathogens are distributed in the environment and the ability of pathogens to form environmental reservoirs is critical to understanding and predicting disease dynamics within host populations. Snake fungal disease (SFD) is an emerging conservation threat to North American snake populations. The causative agent, Ophidiomyces ophidiicola (Oo), is detectable in environmentally derived soils. However, little is known about the distribution of Oo in the environment and the persistence and growth of Oo in soils. Here, we use quantitative PCR to detect Oo in soil samples collected from five snake dens. We compare the detection rates between soils collected from within underground snake hibernacula and associated, adjacent topsoil samples. Additionally, we used microcosm growth assays to assess the growth of Oo in soils and investigate whether the detection and growth of Oo are related to abiotic parameters and microbial communities of soil samples. We found that Oo is significantly more likely to be detected in hibernaculum soils compared to topsoils. We also found that Oo was capable of growth in sterile soil, but no growth occurred in soils with an active microbial community. A number of fungal genera were more abundant in soils that did not permit growth of Oo, versus those that did. Our results suggest that soils may display a high degree of both general and specific suppression of Oo in the environment. Harnessing environmental suppression presents opportunities to mitigate the impacts of SFD in wild snake populations.
Did Politicians Use Non-Public Macroeconomic Information in Their Stock Trades? Evidence from the STOCK Act of 2012
Authors: Karadas Serkan , Schlosky Minh Tam Tammy , Hall Joshua
Published: June 2021

Existing research shows that members of Congress made informed trades prior to the passage of the STOCK Act of 2012. There is also evidence in the literature to suggest that the STOCK Act was able to deter politicians from trading based on non-public information. However, the question of whether politicians made informed trades at the market level (using non-public macroeconomic information, not just firm-specific information) in the first place and whether they continued to do so even after the passage of the STOCK Act remains unexamined. We analyze 101,191 individual stock transactions covering the 2004–2014 period and find that the STOCK Act adversely affected the ability of politicians’ aggregated stock trades to predict the stock market returns. Our results imply that politicians used non-public macroeconomic information prior to the STOCK Act, and this legislation was influential in deterring politicians from using non-public macroeconomic information in their stock trades. Our findings also provide input on the current debate on the need for the STOCK Act 2.0.
A Deep Learning Integrated Cairns-Blake-Dowd (CBD) Sytematic Mortality Risk Model
Authors: Odhiambo Joab , Weke Patrick , Ngare Philip
Published: June 2021

Many actuarial science researchers on stochastic modeling and forecasting of systematic mortality risk use Cairns-Blake-Dowd (CBD) Model (2006) due to its ability to consider the cohort effects. A three-factor stochastic mortality model has three parameters that describe the mortality trends over time when dealing with future behaviors. This study aims to predict the trends of the model, kt(2) by applying the Recurrent Neural Networks within a Short-Term Long Memory (an artificial LSTM architecture) compared to traditional statistical ARIMA (p,d,q) models. The novel deep learning (machine learning) technique helps integrate the CBD model to enhance its accuracy and predictive capacity for future systematic mortality risk in countries with limited data availability, such as Kenya. The results show that Long Short-Term Memory network architecture had higher levels of precision when predicting the future systematic mortality risks than traditional methods. Ultimately, the results can be implemented by Kenyan insurance firms when modeling and forecasting systematic mortality risk helpful in the pricing of Annuities and Assurances.
Role of Artificial Neural Networks Techniques in Development of Market Intelligence: A Study of Sentiment Analysis of eWOM of a Women’s Clothing Company
Authors: Nawaz Zubair , Zhao Chenhui , Nawaz Fouzia , Safeer Asif Ali , Irshad Waseem
Published: June 2021

Web 2.0 technology enables customers to share electronic word of mouth (eWOM) about their experiences. eWOM offers great market insights to the organization, and important for organization’s success. eWOM monitoring and management is one of the major contemporary challenges for the organization, because of high volume and frequency of the content. It is nearly impossible for an organization to manually monitor content generated by each user. In this paper, we propose sentiment analysis as an alternative method for analysis of emotions and behavioral intentions in real-time data. Sentiment analysis is performed on women’s e-clothing reviews collected from the Kaggle data repository. The dataset consists of 23,486 reviews, comprising ten feature variables. This study applied artificial neural network techniques to determine polarity of the data in terms of positive or negative. Sentiment analysis was performed by using two artificial neural networks, Convolutional Neural Network (CNN) and Long Short-Term Memory (LSTM), to classify the review as recommended (positive) or not recommended (negative). The proposed models have been evaluated on these performance measuring parameters: accuracy, recall, specificity, F1-score and roc-curve. The LSTM method outperformed CNN and achieved classification accuracy of 91.69%, specificity 92.81%, sensitivity 76.95%, and 56.67% F1-score. Based on results of this study, LSTM technique is highly recommended for the sentiment analysis of unstructured text-based user-generated content.
Radon Survey in Bank Buildings of Campania Region According to the Italian Transposition of Euratom 59/2013
Authors: D’Avino Vittoria , Pugliese Mariagabriella , Ambrosino Fabrizio , Bifulco Mariateresa , La Commara Marco , Roca Vincenzo , Sabbarese Carlo , La Verde Giuseppe
Published: June 2021

222Rn gas represents the major contributor to human health risk from environmental radiological exposure. In confined spaces radon can accumulate to relatively high levels so that mitigation actions are necessary. The Italian legislation on radiation protection has set a reference value for the activity concentration of radon at 300 Bq/m3. In this study, measurements of the annual radon concentration of 62 bank buildings spread throughout the Campania region (Southern Italy) were carried out. Using devices based on CR-39 solid-state nuclear track detectors, the 222Rn level was assessed in 136 confined spaces (127 at underground floors and 9 at ground floors) frequented by workers and/or the public. The survey parameters considered in the analysis of the results were: floor types, wall cladding materials, number of openings, door/window opening duration for air exchange. Radon levels were found to be between 17 and 680 Bq/m3, with an average value of 130 Bq/m3 and a standard deviation of 120 Bq/m3. About 7% of the results gave a radon activity concentration above 300 Bq/m3. The analysis showed that the floor level and air exchange have the most significant influence. This study highlighted the importance of the assessment of indoor radon levels for work environments in particular, to protect the workers and public from radon-induced health effects.
Quantum Materials: A New Open Section in Materials
Authors: Yang Heesun
Published: June 2021

Quantum Materials is a new open section of Materials aimed at publishing original and review articles on novel scientific and applied research that significantly contribute to the understanding and discovery of quantum materials and related phenomena, functions, and applications [...]
Investigation of Molten Metal Infiltration into Micropore Carbon Refractory Materials Using X-ray Computed Tomography
Authors: Stec Jakub , Tarasiuk Jacek , Wroński Sebastian , Kubica Piotr , Tomala Janusz , Filipek Robert
Published: June 2021

The lifetime of a blast furnace (BF), and, consequently, the price of steel, strongly depends on the degradation of micropore carbon refractory materials used as lining materials in the BF hearth. One of the major degradation mechanisms in the BF hearth is related to the infiltration and dissolution of refractory materials in molten metal. To design new and more resilient materials, we need to know more about degradation mechanisms, which can be achieved using laboratory tests. In this work, we present a new investigation method of refractory materials infiltration resistance. The designed method combines a standard degradation test (hot metal penetration test) with X-ray computed tomography (XCT) measurements. Application of XCT measurements before and after molten metal infiltration allows observing changes in the micropore carbon refractory material’s microstructure and identifying the elements of the open pore structure that are crucial in molten metal infiltration.
Terahertz Photoconductivity Spectra of Electrodeposited Thin Bi Films
Authors: Nevinskas Ignas , Mockus Zenius , Juškėnas Remigijus , Norkus Ričardas , Selskis Algirdas , Norkus Eugenijus , Krotkus Arūnas
Published: June 2021

Electron dynamics in the polycrystalline bismuth films were investigated by measuring emitted terahertz (THz) radiation pulses after their photoexcitation by tunable wavelength femtosecond duration optical pulses. Bi films were grown on metallic Au, Pt, and Ag substrates by the electrodeposition method with the Triton X-100 electrolyte additive, which allowed us to obtain more uniform films with consistent grain sizes on any substrate. It was shown that THz pulses are generated due to the spatial separation of photoexcited electrons and holes diffusing from the illuminated surface at different rates. The THz photoconductivity spectra analysis has led to a conclusion that the thermalization of more mobile carriers (electrons) is dominated by the carrier–carrier scattering rather than by their interaction with the lattice.
Breast Cancer Inhibition by Biosynthesized Titanium Dioxide Nanoparticles Is Comparable to Free Doxorubicin but Appeared Safer in BALB/c Mice
Authors: Iqbal Haroon , Razzaq Anam , Uzair Bushra , Ul Ain Noor , Sajjad Shamaila , Althobaiti Norah Ayidh , Albalawi Aishah Eid , Menaa Bouzid , Haroon Muhammad , Khan Muslim , Khan Naveed Ullah , Menaa Farid
Published: June 2021

Cancer remains a global health burden prompting affordable, target-oriented, and safe chemotherapeutic agents to reduce its incidence rate worldwide. In this study, a rapid, cost-effective, and green synthesis of titanium dioxide (TiO2) nanoparticles (NPs) has been carried out; Ex vivo and in vivo evaluation of their safety and anti-tumor efficacy compared to doxorubicin (DOX), a highly efficient breast anti-cancer agent but limited by severe cardiotoxicity in many patients. Thereby, TiO2 NPs were eco-friendly synthetized using aqueous leaf extract of the tropical medicinal shrub Zanthoxylum armatum as a reducing agent. Butanol was used as a unique template. TiO2 NPs were physically characterized by ultraviolet-visible (UV–Vis) spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM), scanning electron microscope (SEM), X-ray powder diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR) as routine state-of-the art techniques. The synthesized TiO2 NPs were then evaluated for their cytotoxicity (by MTT, FACS, and oxidative stress assays) in 4T1 breast tumor cells, and their hemocompatibility (by hemolysis assay). In vivo anti-tumor efficacy and safety of the TiO2 NPs were further assessed using subcutaneous 4T1 breast BALB/c mouse tumor model. The greenly prepared TiO2 NPs were small, spherical, and crystalline in nature. Interestingly, they were hemocompatible and elicited a strong DOX-like concentration-dependent cytotoxicity-induced apoptosis both ex vivo and in vivo (with a noticeable tumor volume reduction). The underlying molecular mechanism was, at least partially, mediated through reactive oxygen species (ROS) generation (lipid peroxidation). Unlike DOX (P < 0.05), it is important to mention that no cardiotoxicity or altered body weight were observed in both the TiO2 NPs-treated tumor-bearing mouse group and the PBS-treated mouse group (P > 0.05). Taken together, Z. armatum-derived TiO2 NPs are cost-effective, more efficient, and safer than DOX. The present findings shall prompt clinical trials using green TiO2 NPs, at least as a possible alternative modality to DOX for effective breast cancer therapy.
Effect of the PSSMA Content on the Heat Transfer Performances of Polyurea Nano-Encapsulated Phase Change Materials
Authors: Kook Jun-Won , Hwang Kiseob , Lee Jun-Young
Published: June 2021

Polyurea nano-encapsulated phase change materials (PUA-NEPCMs) were prepared from an n-octadecane core and through the formation of amide bonds by the reaction of toluene 2,4-diisocyanate and poly(4-styrenesulfonic acid-co-maleic acid) sodium salt (PSSMA), followed by the subsequent formation of a PUA shell using a miniemulsion system. The effects of the synthetic conditions on the thermal properties and encapsulat ion effect of the NEPCMs were systematically investigated. Differential scanning calorimetry (DSC) revealed that the melting enthalpy and encapsulation efficiency of the PUA-NEPCMs prepared under optimal conditions reached 123.00 J/g and 54.27%, respectively. Although previous results suggested that the introduction of PSSMA results in a reduced heat transfer performance for NEPCMs, DSC analysis of the prepared PUA-NEPCMs showed that increasing PSSMA contents enhanced the heat transfer performance due to a decrease in the degree of supercooling. Our results could therefore lead to further enhancements in the heat transfer performance of PUA-NEPCMs, in addition to expanding their field of application.
Implementation of Digital Technologies into Pre-Service Mathematics Teacher Preparation
Authors: Slavíčková Mária
Published: June 2021

This paper presents a long-term study of Preservice Mathematics Teachers (PMTs) at the Faculty of mathematics, physics and informatics, Comenius University in Bratislava (FMFI UK), focusing on the implementation of digital technologies (DT) into the teaching of theoretical and practical (or applied) subjects. We conducted parallel research into two aspects, one on Calculus lessons as a theoretical subject, another on the Financial Mathematics module as an applied subject. The implementation of DT and the way this was measured varied from year to year and also in the method of implementation into the aforementioned subjects. The methods of implementation and the results are briefly described, and a comparison of these two subjects in the PMTs’ preparation is also discussed.
Oscillation of Second-Order Differential Equations with Multiple and Mixed Delays under a Canonical Operator
Authors: Santra Shyam Sundar , El-Nabulsi Rami Ahmad , Khedher Khaled Mohamed
Published: June 2021

In this work, we obtained new sufficient and necessary conditions for the oscillation of second-order differential equations with mixed and multiple delays under a canonical operator. Our methods could be applicable to find the sufficient and necessary conditions for any neutral differential equations. Furthermore, we proved the validity of the obtained results via particular examples. At the end of the paper, we provide the future scope of this study.
Sphingolipids of Asteroidea and Holothuroidea: Structures and Biological Activities
Authors: Malyarenko Timofey V. , Kicha Alla A. , Stonik Valentin A. , Ivanchina Natalia V.
Published: June 2021

Sphingolipids are complex lipids widespread in nature as structural components of biomembranes. Commonly, the sphingolipids of marine organisms differ from those of terrestrial animals and plants. The gangliosides are the most complex sphingolipids characteristic of vertebrates that have been found in only the Echinodermata (echinoderms) phylum of invertebrates. Sphingolipids of the representatives of the Asteroidea and Holothuroidea classes are the most studied among all echinoderms. In this review, we have summarized the data on sphingolipids of these two classes of marine invertebrates over the past two decades. Recently established structures, properties, and peculiarities of biogenesis of ceramides, cerebrosides, and gangliosides from starfishes and holothurians are discussed. The purpose of this review is to provide the most complete information on the chemical structures, structural features, and biological activities of sphingolipids of the Asteroidea and Holothuroidea classes.
Secondary Metabolite Production Potential of Mangrove-Derived Streptomyces olivaceus
Authors: Hu Dini , Lee Simon Ming-Yuen , Li Kai , Mok Kai Meng
Published: June 2021

Mangroves are intertidal extreme environments with rich microbial communities. Actinobacteria are well known for producing antibiotics. The search for biosynthetic potential of Actinobacteria from mangrove environments could provide more possibilities for useful secondary metabolites. In this study, whole genome sequencing and MS/MS analysis were used to explore the secondary metabolite production potential of one actinobacterial strain of Streptomyces olivaceus sp., isolated from a mangrove in Macau, China. The results showed that a total of 105 gene clusters were found in the genome of S. olivaceus sp., and 53 known secondary metabolites, including bioactive compounds, peptides, and other products, were predicted by genome mining. There were 28 secondary metabolites classified as antibiotics, which were not previously known from S. olivaceus. ISP medium 2 was then used to ferment the S. olivaceus sp. to determine which predicted secondary metabolite could be truly produced. The chemical analysis revealed that ectoine, melanin, and the antibiotic of validamycin A could be observed in the fermentation broth. This was the first observation that these three compounds can be produced by a strain of S. olivaceus. Therefore, it can be concluded that Actinobacteria isolated from the mangrove environment have unknown potential to produce bioactive secondary metabolites.
Blood Pressure Control and Antihypertensive Treatment among Hemodialysis Patients—Retrospective Single Center Experience
Authors: Skonieczny Piotr , Heleniak Zbigniew , Karowiec Marek , Zajączkowski Stanisław , Tylicki Leszek , Dębska-Ślizień Alicja , Rutkowski Przemysław
Published: June 2021

Background and Objectives: Hypertension affects at least 80% of hemodialysis patients. Inappropriate control of blood pressure is mentioned as one of the essential cardiovascular risk factors associated with development of cardiovascular events in dialysis populations. The aim of the cross-sectional, retrospective study was the evaluation of the antihypertensive treatment schedule and control of blood pressure in relation to the guidelines in the group of hemodialysis patients. Additionally, we assessed the level of decrease in blood pressure by each group of hypotensive agents. Materials and Methods: 222 patients hemodialyzed in a single Dialysis Unit in three distinct periods of time—2006, 2011, and 2016—with a diagnosis of hypertension were enrolled in the study. The analysis of the antihypertensive treatment was based on the medical files and it consisted of a comparison of the mean blood pressure results reported during the six consecutive hemodialysis sessions. Results: The mean values of blood pressure before hemodialysis were as follows: 134/77, 130/74, and 140/76 mmHg, after hemodialysis 124/74, 126/73, and 139/77 mmHg in 2006, 2011, and 2016 respectively. The goal of predialysis blood pressure control (<140/90) was achieved by up to 64.3% of participants in 2006 as compared to 49.4% in 2016. Additionally, the postdialysis goal (<130/90) reached 57.1% of the study population in 2006 as compared to 27.1% of patients in 2016. The differences in percentage of patients using single, double, triple, and multidrug therapy during observation were not statistically significant. The most often used drugs were ß-blockers, diuretics, and calcium channel blockers in all points of the study. Blockades of the renin–angiotensin–aldosterone system in 2006 and calcium channel blockers in 2011 and 2016 were the drugs with highest impact on lowering blood pressure. Conclusions: The goal of predialysis or postdialysis blood pressure control was achieved in a lower percentage of patients during the period of the study. Blockade of renin–angiotensin–aldosterone system and calcium channel blockers decrease the blood pressure significantly. It is necessary to achieve better control of blood pressure in prevention of cardiovascular incidents.
Genetic and Environmental Structure of Altruism Characterized by Recipients in Relation to Personality
Authors: Ando Juko , Kawamoto Tetsuya
Published: June 2021

Background and Objectives: Altruism is a form of prosocial behavior with the goal of increasing the fitness of another individual as a recipient while reducing the fitness of the actor. Although there are many studies on its heterogeneity, only a few behavioral genetic studies have been conducted to examine different recipient types: family members favored by kin selection, the dynamic network of friends and acquaintances as direct reciprocity, and strangers as indirect reciprocity. Materials and Methods: This study investigated the genetic and environmental structure of altruism with reference to recipient types measured by the self-report altruism scale distinguished by the recipient (the SRAS-DR) and examine the relationship to personality dimensions measured by the NEO-FFI with a sample of 461 adult Japanese twin pairs. Results: The present study shows that there is a single common factor of altruism: additive genetic effects explain 51% of altruism without a shared environmental contribution. The genetic contribution of this single common factor is explained by the genetic factors of neuroticism (N), extraversion (E), openness to experience (O), and conscientiousness (C), as well as a common genetic factor specific to altruism. Only altruism toward strangers is affected by shared environmental factors. Conclusions: Different types of altruistic personality are constructed by specific combinational profiles of general personality traits such as the Big Five as well as a genetic factor specific to altruism in each specific way.
Enhanced Electrochemical Performance of Li4Ti5O12 by Niobium Doping for Pseudocapacitive Applications
Authors: Chandrasekhar Jinka , Dhananjaya Merum , Hussain Obili M. , Mauger Alain , Julien Christian M.
Published: June 2021

Niobium-doped nanocrystalline Li4Ti5O12 (LTO) is synthesized by the solid-state reaction method, and the influence of dopant concentration (x = 2–10 mol%) on microstructural and electrochemical properties is studied. The X-ray diffraction and Raman patterns assessed the cubic spinel structure of Li4Ti5−xNbxO12 phase in all samples. Marginal changes in the lattice parameters, unit cell volume and dislocation density of LTO are observed with Nb substitution. The higher ionic radius of Nb induces a lattice expansion, which may be favorable for more ion intercalation/deintercalation. The SEM and TEM images display uniformly distributed nano-sized cubical particles. The represented (hkl) orientations of the SAED pattern and d-spacing (0.46 nm) between bright fringes confirm the well-crystallized LTO phase. The EDS and elemental mapping results demonstrate that Nb elements are uniformly doped in LTO with a proper stoichiometric ratio. The optimized 8%Nb-doped LTO electrode exhibits pseudocapacitive behavior and delivers a high specific capacitance of 497 F g−1 at a current density of 1 A g−1 with 92.3% of specific capacitance retention even after 5000 cycles.
Antioxidant, Anti-Inflammatory, and Anti-Diabetic Activity of Phenolic Acids Fractions Obtained from Aerva lanata (L.) Juss.
Authors: Pieczykolan Aleksandra , Pietrzak Wioleta , Gawlik-Dziki Urszula , Nowak Renata
Published: June 2021

Many plants that are commonly used in folk medicine have multidirectional biological properties confirmed by scientific research. One of them is Aerva lanata (L.) Juss. (F. Amaranthaceae). It is widely used, but there are very few scientific data about its chemical composition and pharmacological activity. The aim of the present study was to investigate the chemical composition of phenolic acid (PA)-rich fractions isolated from methanolic extracts of A. lanata (L.) Juss. herb using the liquid/liquid extraction method and their potential antioxidant, anti-inflammatory, and anti-diabetic properties. The free PA fraction (FA), the PA fraction (FB) released after acid hydrolysis, and the PA fraction (FC) obtained after alkaline hydrolysis were analysed using liquid chromatography/electrospray ionization triple quadrupole mass spectrometry (LC-ESI-MS/MS). The phenolic profile of each sample showed a high concentration of PAs and their presence in A. lanata (L.) Juss. herb mainly in bound states. Thirteen compounds were detected and quantified in all samples, including some PAs that had not been previously detected in this plant species. Bioactivity assays of all fractions revealed high 2,2-diphenyl-1-picrylhydrazyl (DPPH•) (2.85 mM Trolox equivalents (TE)/g) and 2,2-azino-bis-3(ethylbenzthiazoline-6-sulphonic acid) (ABTS•+) (2.88 mM TE/g) scavenging activity. Fraction FB definitely exhibited not only the highest antiradical activity but also the strongest xanthine oxidase (XO) (EC50 = 1.77 mg/mL) and lipoxygenase (LOX)(EC50 = 1.88 mg/mL) inhibitory potential. The fraction had the best anti-diabetic properties, i.e., mild inhibition of α-amylase (EC50 = 7.46 mg/mL) and strong inhibition of α-glucosidase (EC50 = 0.30 mg/mL). The activities of all analysed samples were strongly related to the presence of PA compounds and the total PA content.
Enhanced Cytotoxic Effect of Doxorubicin Conjugated to Glutathione-Stabilized Gold Nanoparticles in Canine Osteosarcoma—In Vitro Studies
Authors: Małek Anna , Taciak Bartłomiej , Sobczak Katarzyna , Grzelak Agnieszka , Wójcik Michał , Mieczkowski Józef , Lechowski Roman , Zabielska-Koczywąs Katarzyna A.
Published: June 2021

Osteosarcoma (OSA) is the most common malignant bone neoplasia in humans and dogs. In dogs, treatment consists of surgery in combination with chemotherapy (mostly carboplatin and/or doxorubicin (Dox)). Chemotherapy is often rendered ineffective by multidrug resistance. Previous studies have revealed that Dox conjugated with 4 nm glutathione-stabilized gold nanoparticles (Au-GSH-Dox) enhanced the anti-tumor activity and cytotoxicity of Dox in Dox-resistant feline fibrosarcoma cell lines exhibiting high P-glycoprotein (P-gp) activity. The present study investigated the influence of Au-GSH-Dox on the canine OSA cell line D17 and its relationship with P-gp activity. A human Dox-sensitive OSA cell line, U2OS, served as the negative control. Au-GSH-Dox, compared to free Dox, presented a greater cytotoxic effect on D17 (IC50 values for Au-GSH-Dox and Dox were 7.9 μg/mL and 15.2 μg/mL, respectively) but not on the U2OS cell line. All concentrations of Au-GSH (ranging from 10 to 1000 μg/mL) were non-toxic in both cell lines. Inhibition of the D17 cell line with 100 μM verapamil resulted in an increase in free Dox but not in intracellular Au-GSH-Dox. The results indicate that Au-GSH-Dox may act as an effective drug in canine OSA by bypassing P-gp.
The DEPTQ+ Experiment: Leveling the DEPT Signal Intensities and Clean Spectral Editing for Determining CHn Multiplicities
Authors: Bigler Peter , Melendez Camilo , Furrer Julien
Published: June 2021

We propose a new 13C DEPTQ+ NMR experiment, based on the improved DEPTQ experiment, which is designed to unequivocally identify all carbon multiplicities (Cq, CH, CH2, and CH3) in two experiments. Compared to this improved DEPTQ experiment, the DEPTQ+ is shorter and the different evolution delays are designed as spin echoes, which can be tuned to different 1JCH values; this is especially valuable when a large range of 1JCH coupling constants is to be expected. These modifications allow (i) a mutual leveling of the DEPT signal intensities, (ii) a reduction in J cross-talk in the Cq/CH spectrum, and (iii) more consistent and cleaner CH2/CH3 edited spectra. The new DEPTQ+ is expected to be attractive for fast 13C analysis of small-to medium sized molecules, especially in high-throughput laboratories. With concentrated samples and/or by exploiting the high sensitivity of cryogenically cooled 13C NMR probeheads, the efficacy of such investigations may be improved, as it is possible to unequivocally identify all carbon multiplicities, with only one scan, for each of the two independent DEPTQ+ experiments and without loss of quality.
The Identification of New Triterpenoids in Eucalyptus globulus Wood
Authors: Lourenço Ana , Marques António Velez , Gominho Jorge
Published: June 2021

Eight polyhydroxy triterpenoid acids, hederagenin, (4α)-23-hydroxybetulinic acid, maslinic acid, corosolic acid, arjunolic acid, asiatic acid, caulophyllogenin, and madecassic acid, with 2, 3, and 4 hydroxyl substituents, were identified and quantified in the dichloromethane extract of Eucalyptus globulus wood by comparing their GC-retention time and mass spectra with standards. Two other triterpenoid acids were tentatively identified by analyzing their mass spectra, as (2α)-2-hydroxybetulinic acid and (2α,4α)-2,23-dihydroxybetulinic acid, with 2 and 3 hydroxyl substituents. Two MS detectors were used, a quadrupole ion trap (QIT) and a quadrupole mass filter (QMF). The EI fragmentation pattern of the trimethylsilylated polyhydroxy structures of these triterpenoid acids is characterized by the sequential loss of the trimethylsilylated hydroxyl groups, most of them by the retro-Diels-Alder (rDA) opening of the C ring with a π-bond at C12-C13. The rDA C-ring opening produces ions at m/z 320 (or 318) and m/z 278 (or 277, 276, 366). Sequential losses of the hydroxyl groups produce ions with m/z from [M - 90] to [M - 90*y], where y is the number of hydroxyl substituents present (from 2 to 4). Moreover, specific cleavage in ring E was observed, passing from m/z 203 to m/z 133 and conducting other major fragments such as m/z 189.
Silylated Tag-Assisted Peptide Synthesis: Continuous One-Pot Elongation for the Production of Difficult Peptides under Environmentally Friendly Conditions
Authors: Yano Shinya , Mori Toshihiro , Kubota Hideki
Published: June 2021

Addition of the silylated tag (STag) enables peptides to be highly soluble in CPME, allowing them to be used at high concentrations in a coupling reaction to enhance reactivity and achieve effective synthesis of sterically hindered peptides. We described the development of a continuous one-pot STag-assisted peptide synthesis platform as a method that provides near-stoichiometric, speedy, environmentally friendly, and scalable peptide synthesis.
Intake of Vitamin E and C in Women of Reproductive Age: Results from the Latin American Study of Nutrition and Health (ELANS)
Authors: Busso Dolores , David Andrea , Penailillo Reyna , Echeverría Guadalupe , Rigotti Attilio , Kovalskys Irina , Gómez Georgina , Cortés Sanabria Lilia , Yépez García Martha , Pareja Rossina , Herrera-Cuenca Marianella , Fisberg Mauro ,   on behalf of the ELANS Study Group
Published: June 2021

Vitamin E was identified as a lipophilic compound essential to maintain rat pregnancy. Low vitamin E intake during early pregnancy associates with congenital malformations and embryonic loss in animals and with miscarriage and intrauterine growth restriction in humans. Vitamin E protects cell membranes from lipoperoxidation and exerts non-antioxidant activities. Its function can be restored by vitamin C; thus, intake and circulating levels of both micronutrients are frequently analyzed together. Although substantial vitamin E inadequacy was reported worldwide, its consumption in Latin America (LatAm) is mostly unknown. Using data from the Latin American Study of Nutrition and Health (Estudio Latinoamericano de Nutrición y Salud, ELANS), we evaluated vitamin E and C intake in women of reproductive age (WRA) from eight LatAm countries and identified their main food sources. Two non-consecutive 24-h dietary recalls in 3704 women aged from 15 to 49 years and living in urban locations showed low average intake of vitamin E (7.9 mg/day vs. estimated average requirement (EAR) of 12 mg/day) and adequate overall vitamin C consumption (95.5 mg/day vs. EAR of 60 mg/day). The mean regional inadequacy was 89.6% for vitamin E and 36.3% for vitamin C. The primary food sources of vitamin E were fats and oils, as well as vegetables. Vitamin C intake was explained mainly by the consumption of fruit juices, fruits, and vegetables. Combined deficient intake of both vitamins was observed in 33.7% of LatAm women. Although the implications of low antioxidant vitamins’ consumption in WRA are still unclear, the combined deficient intake of both vitamins observed in one-third of ELANS participants underscores the need for further research on this topic.
Aspartame—True or False? Narrative Review of Safety Analysis of General Use in Products
Authors: Czarnecka Kamila , Pilarz Aleksandra , Rogut Aleksandra , Maj Patryk , Szymańska Joanna , Olejnik Łukasz , Szymański Paweł
Published: June 2021

Aspartame is a sweetener introduced to replace the commonly used sucrose. It was discovered by James M. Schlatter in 1965. Being 180–200 times sweeter than sucrose, its intake was expected to reduce obesity rates in developing countries and help those struggling with diabetes. It is mainly used as a sweetener for soft drinks, confectionery, and medicines. Despite its widespread use, its safety remains controversial. This narrative review investigates the existing literature on the use of aspartame and its possible effects on the human body to refine current knowledge. Taking to account that aspartame is a widely used artificial sweetener, it seems appropriate to continue research on safety. Studies mentioned in this article have produced very interesting results overall, the current review highlights the social problem of providing visible and detailed information about the presence of aspartame in products. The studies involving the impact of aspartame on obesity, diabetes mellitus, children and fetus, autism, neurodegeneration, phenylketonuria, allergies and skin problems, its cancer properties and its genotoxicity were analyzed. Further research should be conducted to ensure clear information about the impact of aspartame on health.
Influence of the COVID-19 Pandemic on the Lifestyles of Health Sciences University Students in Spain: A Longitudinal Study
Authors: Imaz-Aramburu Idoia , Fraile-Bermúdez Ana-Belén , Martín-Gamboa Batirtze San , Cepeda-Miguel Silvia , Doncel-García Borja , Fernandez-Atutxa Ainhoa , Irazusta Amaia , Zarrazquin Idoia
Published: June 2021

The COVID-19 pandemic has significantly impacted daily activities worldwide. University students may have experienced substantial changes in daily living as a result of restrictions on university attendance. The return to normalcy may take a long time, and understanding the influence that shifts in daily routines have had on the lifestyles of university students may inform approaches to support overall well-being. We analyzed changes in the lifestyles of students enrolled at a health sciences university during the COVID-19 pandemic. This longitudinal study took place at the Faculty of Medicine and Nursing in the University of the Basque Country in Spain, and the final sample consisted of 113 nursing students, 109 medical students, and 45 physiotherapy students. Our results demonstrate changes in lifestyles of university students during the pandemic. MedDiet adherence scores and the percentage of students with high adherence increased during the pandemic. This increase was due to the increased consumption of vegetables and nuts. In terms of physical activity, the practice of moderate and intense physical activity was maintained. These results provide important information for both public health authorities and educational institutions to guide strategies to maintain the well-being of students and enhance opportunities for young adults to lead a healthy lifestyle.
Social Image of Nursing. An Integrative Review about a Yet Unknown Profession
Authors: López-Verdugo Macarena , Ponce-Blandón Jose Antonio , López-Narbona Francisco Javier , Romero-Castillo Rocío , Guerra-Martín María Dolores
Published: June 2021

Background: Nursing is a discipline on which stereotypes have persisted throughout its history, considering itself a feminine profession and subordinated to the medical figure, without its own field of competence. All this leads to an image of the Nursing Profession that moves away from reality, constituting a real, relevant and high-impact problem that prevents professional expansion, and that has a direct impact on social trust, the allocation of resources and quality of care, as well as wages and professional satisfaction. The aim of this review was to identify and publicize the published material on the social image of Nursing, providing updated information about the different approaches to the subject. Methods: An integrative review of the literature has been made from primary sources of information published from 2010 to 2020. For this, the databases CINAHL, Scopus, SciELO, Dialnet and Cuiden have been consulted. Results: In total, 17 articles have been included in the review, with qualitative, quantitative, and even bibliographic reviews performed in countries such as Spain, Egypt, Argentina, Iran, Venezuela, Turkey, United Kingdom, and Australia. The results of those papers mostly showed that society has misinformation about the functions performed by nursing professionals, which is based on myths and stereotypes. Conclusions: Nursing is a profoundly unknown and invisible profession, as society continues without recognizing its competence, autonomy and independence.
Relating Charged Particle Multiplicity to Impact Parameter in Heavy-Ion Collisions at NICA Energies
Authors: Parfenov Petr , Idrisov Dim , Luong Vinh Ba , Taranenko Arkadiy
Published: June 2021

The size and evolution of the matter created in relativistic heavy-ion collisions strongly depend on collision geometry, defined by the impact parameter. However, the impact parameter cannot be measured directly in an experiment but might be inferred from final state observables using the centrality procedure. We present the procedure of centrality determination for the Multi-Purpose Detector (MPD) at the NICA collider and its performance using the multiplicity of produced charged particles at midrapidity. The validity of the procedure is assessed using the simulated data for Au + Au collisions at sNN = 4–11 GeV.
Meta-Assessment of Metformin Absorption and Disposition Pharmacokinetics in Nine Species
Authors: Jeong Yoo-Seong , Jusko William J.
Published: June 2021

The objective of this study was to systematically assess literature datasets and quantitatively analyze metformin PK in plasma and some tissues of nine species. The pharmacokinetic (PK) parameters and profiles of metformin in nine species were collected from the literature. Based on a simple allometric scaling, the systemic clearances (CL) of metformin in these species highly correlate with body weight (BW) (R2 = 0.85) and are comparable to renal plasma flow in most species except for rabbit and cat. Reported volumes of distribution (VSS) varied appreciably (0.32 to 10.1 L/kg) among species. Using the physiological and anatomical variables for each species, a minimal physiologically based pharmacokinetic (mPBPK) model consisting of blood and two tissue compartments (Tissues 1 and 2) was used for modeling metformin PK in the nine species. Permeability-limited distribution (low fd1 and fd2) and a single tissue-to-plasma partition coefficient (Kp) value for Tissues 1 and 2 were applied in the joint mPBPK fitting. Nonlinear regression analysis for common tissue distribution parameters along with species-specific CL values reasonably captured the plasma PK profiles of metformin across most species, except for rat and horse with later time deviations. In separate fittings of the mPBPK model to each species, Tissue 2 was considered as slowly-equilibrating compartment consisting of muscle and skin based on in silico calculations of the mean transit times through tissues. The well-fitted mPBPK model parameters for absorption and disposition PK of metformin for each species were compared with in vitro/in vivo results found in the literature with regard to the physiological details and physicochemical properties of metformin. Bioavailability and absorption rates decreased with the increased BW among the species. Tissues such as muscle dominate metformin distribution with low permeability and partitioning while actual tissue concentrations found in rats and mice show likely transporter-mediated uptake in liver, kidney, and gastrointestinal tissues. Metformin has diverse pharmacologic actions, and this assessment revealed allometric relationships in its absorption and renal clearance but considerable variability in actual and modeled tissue distribution probably caused by transporter differences.
Therapeutic Potential of Glycosyl Flavonoids as Anti-Coronaviral Agents
Authors: Godinho Patrícia I. C. , Soengas Raquel G. , Silva Vera L. M.
Published: June 2021

The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has spread all over the world, creating a devastating socio-economic impact. Even though protective vaccines are starting to be administered, an effective antiviral agent for the prevention and treatment of COVID-19 is not available yet. Moreover, since new and deadly CoVs can emerge at any time with the potential of becoming pandemics, the development of therapeutic agents against potentially deadly CoVs is a research area of much current interest. In the search for anti-coronaviral drugs, researchers soon turned their heads towards glycosylated flavonoids. Glycosyl flavonoids, widespread in the plant kingdom, have received a lot of attention due to their widely recognized antioxidant, anti-inflammatory, neuroprotective, anticarcinogenic, antidiabetic, antimicrobial, and antiviral properties together with their capacity to modulate key cellular functions. The wide range of biological activities displayed by glycosyl flavonoids, along with their low toxicity, make them ideal candidates for drug development. In this review, we examine and discuss the up-to-date developments on glycosyl flavonoids as evidence-based natural sources of antivirals against coronaviruses and their potential role in the management of COVID-19.