bluuebunny/crossref_metadata_2025 huggingface dataset:
BUILDING AND DEVELOPING VIETNAMESE CULTURE: FROM POLICY TO PRACTICE
Authors: Thi Thanh Trinh
Published: September 2023

Culture plays an important role in the spiritual life of Vietnamese people. In the process of national construction and development, as a ruling party, the Communist Party of Vietnam has been aware of the position, role, and importance of culture. In many of its policies, the Communist Party of Vietnam has issued many guidelines and guidelines for building and developing Vietnam's culture. In particular, in recent years, many policies on culture have been promulgated and gradually entered into practice, bringing many practical results to the construction of people's lives and participation in building and developing the country. This study clearly shows the cultural policy of Vietnam in history, the process of penetrating into real life and proposes solutions to continue building and developing an advanced Vietnamese culture imbued with ​identity nation.
The plague in Poljice in 1783
Authors: Kocić Marija
Published: September 2023

Due to its unique geographical position, Poljice represents an area that has retained certain autochthonous features for a long time and has accepted the Ottoman and then Venetian influences to a minimal extent. That had the effect that Poljice became an area with special status. Only on its territory the Venetian authorities did not appoint an official (provveditore or superintendent), as was the case in the parts of Dalmatia that belonged to the Republic based on the demarcation carried out in 1699 and 1718. Without a representative of the central government, residents did not readily accept its regulations, including sanitary ones. This fact determined this research, which tries to show how the plague epidemic was suppressed in the absence of state sanitary authorities in 1783, which was remembered as one of the most severe that hit Dalmatia in that century.
Impact of Dexamethasone on the stress response during perioperative management of elderly patients with hip fracture
Authors: Šakić Livija , Šakić-Zdravčević Kata
Published: September 2023

Introduction: Hip fracture is the most common cause of emergency hospital admissions among the elderly population and is associated with high postoperative morbidity. The hypothesis is that a single intrathecal dose of Dexamethasone with Levobupivacaine for surgical correction of hip fracture reduces surgical stress and contributes to better treatment outcomes. Method: Elderly patients with hip fracture who underwent surgery under spinal anesthesia with Levobupivacaine were analyzed, with one group receiving a single intrathecal dose of Dexamethasone and the other group serving as a control (group DLSA/control group LSA). The following variables were monitored: gender, type of injury, cortisol levels, time elapsed from injury to surgical intervention, pain intensity, occurrence of delirium, postoperative cognitive deficits and other complications, length of hospital stay, and mortality. Results: Reduced cortisol concentrations, prolonged analgesia duration, and shorter hospitalization duration were observed in DLSA group, compared to the LSA group. Conclusion: A single intrathecal injection of Dexamethasone in spinal anesthesia for surgical correction of hip fracture reduces perioperative stress response by lowering plasma cortisol concentration, providing a longer analgesic effect, reducing the incidence of delirium and POCD (Postoperative Cognitive Dysfunction), and improving rehabilitation prospects. This leads to a shorter hospitalization duration, justifying this anesthetic approach as a recommended method of choice.
Corticosteroid use in critically ill patients
Authors: Šurbatović Maja , Rakić Goran , Jevđić Jasna
Published: September 2023

The use of corticosteroids as immunosuppressants in critically ill patients is a very complex issue. These potent immunomodulators can be used in the treatment of critically ill patients with severe community-acquired pneumonia (SCAP) and/or septic shock. Local and systemic inflammatory responses are increased in SCAP, thus impairing gas exchange. Also, persistent inflammatory response is associated with higher mortality in patients with COVID-19 and non-COVID-19-related ARDS. However, the risk of administering systemic steroids (methylprednisolone, Dexamethasone, hydrocortisone, etc.) to non-responders is evident. In general, the use of corticosteroids in critically ill patients should not be routine (one size fits all approach) but as personalized and individualized as clinically possible (from one size does not fit all to one size fits one).
Chronic obstructive pulmonary disease and osteoporosis
Authors: Janković Jelena , Buha Ivana , Jandrić Aleksandar , Opačić Jasmina
Published: September 2023

Chronic obstructive pulmonary disease (COPD) is a progressive disease characterized by broncho-obstruction, loss of lung elasticity and parenchymal destruction. Today, this disease is the third cause of death in the world. Smoking is the main risk factor for this disease, while other risk factors are vitamin D deficiency, physical inactivity, and the use of systemic corticosteroids. Osteoporosis is a very common comorbidity in patients with COPD and is characterized by lower bone density and changes in the microarchitecture of the bone, which leads to fracture. These two diseases have the same pathophysiological mechanism of origin based on inflammation and common risk factors. If fractures occur, they can further worsen the already decreased pulmonary ventilation, reduce mobility and lead to disability. In patients with COPD it should be taken into consideration that there is a high likelihood of coexisting osteoporosis. Therefore, it is important to timely undertake appropriate diagnostic and preventative measures to prevent the progression of this condition
Integrating experimental analysis and machine learning for assessing bond performance and corrosion severity in reinforced concrete structures
Authors: Almashakbeh Yousef
Published: September 2023

This study presents a comprehensive investigation into the effects of corrosion on bond performance and mechanical properties of steel-concrete interfaces by combining experimental analysis with machine learning techniques. A total of 32 concrete prisms with varying water-cement (w/c) ratios were prepared and subjected to an accelerated corrosion process. Corrosion severity was assessed through visual inspection and weight loss measurements, while mechanical properties were evaluated through the pull-out tests. Experimental results showed that the residual bond load decreased to 77% and 81% for w/c ratios of 0.37 and 0.47, respectively, after accelerated corrosion. Additionally, corroded prisms exhibited significantly reduced residual toughness and stiffness compared to their non-corroded counterparts. To establish a correlation between bond slip and corrosion severity, a machine learning algorithm was developed and implemented. The algorithm achieved an accuracy of 100% for both studied w/c ratios and remarkably low costs after optimization (4.548× 10 -6 for w/c ratio of 0.37 and 3.445× 10 -7 for w/c ratio of 0.47). This integrated approach provides valuable insights for future infrastructure assessment and maintenance efforts. In conclusion, this study combines laboratory findings with real-world applications. It provides a thorough understanding of the relationship between corrosion and bond performance in reinforced concrete structures. Maintaining the structural integrity and safety of reinforced concrete structures in corrosive environments can be aided by the results of this research).
FUNGSI GAYA BAHASA PERBANDINGAN DAN PERTENTANGAN DALAM PODCAST RINTIK SEDU PADA APLIKASI SPOTIFY
Authors: Risnawati Risnawati , Usman Usman , Hasriani Hasriani
Published: September 2023

The Function of Comparative and Contradictory Language Styles in the Rintik Sedu Podcast on the Spotify application obtains two comparative functions, namely conveying similarities and comparative statements, as well as obtaining two contradictory functions, namely, conveying affirmation and conveying opinions. Conveying similarities is used to express similarities to something in question. Comparative statements are used to express a comparison of something. Conveying an affirmation is used to emphasize something that is considered to be in accordance with the speaker's opinion. Expressing opinions is used to provide advice or opinions on something to someone. This research aims to describe the function of comparative and contradictory language styles in the rintik sedu podcast on the Spotify application. This research uses a qualitative descriptive method obtained through note-taking, reading and documentation techniques. The results of the research found five data with one statement each, two statements of similarities and two statements of comparison
ESTUDOS DECOLONIAIS: PARTICIPAÇÃO DA JUVENTUDE INDÍGENA NAS MÍDIAS DIGITAIS E NO CONTEXTO DA HISTÓRIA SOCIAL
Authors: Kaingang Tadeu Tãn Dó
Published: September 2023

Pretendo abordar um diagnóstico da participação da juventude indígena, pinçamos documentos que estão disponíveis na   mídia digital que servem para ilustra nossa reflexão, nas narrativas colônias em contraste por exemplo, do grupo Nen Ga, Kaingang do Paraná, criado em 2014, e do coletivo Kókir de 2016, sobre o aspecto da arte  e cultura ao tema humanidades digitais e estudos decoloniais. Com base nos estudos decoloniais, de Quijano (1990), Mignolo(2017) e Walsh (2009), permitem refletir sobre a desconstrução do pensamento eurocêntrico.
HISTÓRIA DO EUROCENTRISMO A PARTIR DA PLATAFORMA GOOGLE NGRAM
Authors: Nunes Macedo André Luan
Published: September 2023

O presente artigo analisa a história do conceito de eurocentrismo a partir da plataforma Google Ingram. Pesquisei a palavra em inglês e espanhol, criando assim dois bancos de dados a serem analisados. Afinal, quando escrevo a palavra “eurocentrismo” trata-se de uma palavra qualquer e que historicamente não possui relevância suficiente que sustente um maior fôlego investigativo em termos de sentido temporal? Ou estamos diante de um conceito, capaz de sustentar uma perspectiva genérica quanto ao seu significado e, ao mesmo tempo, possuir um sentido polissêmico? Tenho como objetivo traçar os seus diferentes sentidos ao longo do tempo. Trata-se de entender as razões pelas quais dos primeiros aparecimentos da palavra por meio de uma leitura do “clima histórico” da época. O outro prisma qualitativo se relaciona à leitura de artigos e livros que se utilizam da palavra ao longo do tempo, procurando, assim, comparar diferentes épocas e diferentes sentidos atribuídos ao conceito em questão.
EXPLORANDO OS POTENCIAIS E ENFRENTANDO OS DESAFIOS PARA O USO DO CHATGPT EM SALA DE AULA: DESAFIOS HISTÓRICOS E PEDAGÓGICOS
Authors: Carvalho Pedro Eduardo Andrade
Published: September 2023

No presente artigo, avalia as potencialidades do uso da plataforma ChatGPT para o ensino de história nas turmas dos ensinos fundamental em médio no Brasil. Para alguns, a plataforma ameaça o atual modelo de ensino frente a frente entre professores e alunos uma vez que entrega aos educandos um modelo de bate-papo digital que responde perguntas de variados temas, incluindo, aqueles discutidos em sala de aula. Os mais realistas veem que a plataforma, de fato, não constitui em ameaça para os professores, mas em alternativa didático-pedagógica a fim de retomar a atenção dos alunos, hoje, dispersa nos smartphones. Neste sentido, proponho algumas formas para a utilização da plataforma, tanto na elaboração das aulas quanto nas atividades dentro de sala e para sugerir possíveis dinâmicas com os alunos.
INTERCULTURALIDADE CABE NO DIREITO? A NEGAÇÃO DAS DIVERSIDADES NO ÂMBITO DO ESTADO, AS POSSIBILIDADES NO PROCESSO CONSTITUINTE BRASILEIRO DE 1988 E A ALTERNATIVA DESDE O NOVO CONSTITUCIONALISMO DEMOCRÁTICO LATINO-AMERICANO
Authors: Martins Lemes João Vitor , Fernandes Silva Joana Aparecida
Published: September 2023

A questão da diversidade cultural se apresenta enquanto central ao campo científico a partir do reconhecimento dos processos de colonização e descolonização, que demandam aos Estados latino-americanos a tarefa de construir meios de garantir a convivência democrática entre as diferentes culturas, buscando a integração entre elas sem alterar sua diversidade. Nesse sentido, este trabalho objetiva discutir quais seriam as alternativas que efetivam o reconhecimento que essas coletividades impõem, identificando, assim, quais seriam as possibilidades para a construção de um Estado em que caiba a noção de interculturalidade, ou, reconhecendo o direito enquanto braço do Estado na regulação das relações sociais e culturais, de uma interculturalidade que caiba do Direito.
CORRELAÇÃO ENTRE A DEPENDÊNCIA DIGITAL E O ESTILO DE VIDA ATIVO DE UNIVERSITÁRIOS CEARENSES PERTENCENTES A ÁREA DA SAÚDE
Authors: Silvestre Nogueira Pedro Henrique , Martins Raphaell Moreira
Published: September 2023

A dependência digital é um fenômeno complexo que pode provocar o não cuidado com a saúde. Sendo assim, o objetivo deste estudo foi correlacionar os níveis de Dependência Digital com o estilo de vida ativo de universitários pertencentes à área da saúde. Trata-se, portanto, de um estudo correlacional, realizado com 166 universitários. Foi identificado que todos eles possuem algum tipo de dependência digital. Houve uma correlação estatisticamente fraca entre tal dependência e o nível de atividade física. Depreende-se que estes sujeitos, em termos gerais, possuem uma concepção de cuidado com o corpo mais consciente. Conclui-se que este tema precisa ser melhor debatido sem a tendência proibicionista que vigora em muitos casos por considerar as tecnologias como inimigas do estilo de vida ativo.
HISTÓRIA, PODCAST E FORMAÇÃO DE PROFESSORES: O USO DE PODCAST NO ENSINO DE HISTÓRIA, EXPERIMENTAÇÕES DO PIBIDHISTÓRIA-UFCAT
Authors: HARUME FIGUEIREDO INOUE SAMANTHA , Vieira Nunes Radamés
Published: September 2023

Esse artigo propõe uma reflexão sobre a importância e possibilidades do podcast no Ensino de História, especialmente nos anos finais do Ensino Fundamental II. Abordamos a relação entre tecnologias digitais, sociedade, escola e aluno. A tecnologia que possibilita o uso do podcast pode ser adaptada ao Ensino de História, deixando as aulas mais didáticas, interativas e fazendo com que o conhecimento histórico produzido no espaço escolar seja divulgado de forma mais ampla. A proposta desenvolve-se a partir de uma experiência vivenciada junto ao Programa Institucional de Bolsas de Iniciação à Docência (PIBID), no subprojeto de História da Universidade Federal de Catalão (UFCAT), edição de 2020-2021, quando utilizamos o podcast, como ferramenta para as aulas de História na Escola Instituto Matilde Margon Vaz, instituição da rede pública estadual de Educação Básica. O podcast mostrou-se como importante aliado para o ensino e para aprendizagem de história, por isso deve ser, como outras tecnologias digitais, pensado e experimentado como ferramenta em programas e cursos de formação de professores.
Instructional Designers as Ethical Mediators
Authors: Gray Colin M.
Published: September 2023

Instructional designers have been instrumental in shaping learning experiences for almost a century—contributing to perceptions of what instructional experiences should be considered valuable, worthwhile, and rigorous. However, instructional theories and models of instructional design practice have rarely considered the ethical role of the designer in creating equitable and inclusive futures. In this chapter, I use two vignettes of instructional design work framed by facial recognition technologies to locate ethical tensions between designers and learners, identifying opportunities to leverage the mediating role of the designer. I describe potential ways forward for researchers, educators, and students that reposition ethics at the core of the discipline.
In Support of Ethical Instructional Design
Authors: Warren Scott , Beck Dennis , McGuffin Kristen
Published: September 2023

As technology solutions continue to grow in complexity, the choices facing those who wish to use them both effectively and ethically continue to grow more complex. The purpose of this chapter is to present the Ethical Choices with Educational Technology framework translated from K-12 setting use to instructional design practices in any setting (ECET ID). Two competing instructional design tool resources are compared and scored using the ECET ID framework to illustrate how it can help a designer choose a multimedia production tool that a.) meets the needs of their idea, b.) is feasible to use by their clients in the time available, and c.) is deemed to have the best ethical outcomes from design through use.
Assessing the Variations in Canopy Temperature, Relative Humidity and Soil Temperature within the Canopy of Winter Maize in Bihar
Authors: Sharma Ankit , Sattar Abdus , Kumar Mritunjay , Jha Ratnesh Kumar , Kumar Ravi Ranjan , Singh Gulab
Published: September 2023

Variations in canopy temperature, relative humidity and soil temperature within crop micro-environment exert full control over plant’s physiological processes and thereby over growth, development and yield of crop. These micro-meteorological parameters tend to significantly control the exchange of energy between the crop environment and the atmosphere. Keeping all this in view, a field experiment with winter maize ( var. Rajendra hybrid 4) was conducted with five dates of sowing viz., 1 November (D1), 10 November (D2), 20 November (D3), 30 November (D4) and 10 December (D5) and three spacings viz., 60 X 20 cm (S1), 75 cm X 20 cm (S2) and 45 cm X 20 cm (S3) at the University farm of Dr Rajendra Prasad Central Agricultural University, Pusa, Samastipur, Bihar during rabi season of 2021-22 to assess the variations in canopy temperature, relative humidity and soil temperature, as influenced by micro-environments and planting density. The experiment was laid out in split plot design with sowing dates as main-plots and spacings as split plots. Results revealed that canopy temperature was found to be the highest for the crop planted on D4 (30 November) with S2 (75cm X 20 cm) spacing, while the lowest was associated with the crop sown on D1 (1 November) with S3 (45 cm X 20 cm) spacing. Mean canopy temperature decreased from sowing to knee high stage, thereafter it increased with every phenological stage up to physiological maturity. Crop sown on D3 (20 November) having S3 (45 cm X 20 cm) spacing recorded maximum relative humidity. Highest soil temperature at 15 cm depth was recorded for D5 (10 December) sown and S2 (75cm X 20cm) spaced crop and the lowest for D2 (10 November) having S3 (45cm X 20cm) spacing. Information generated on the variation in canopy temperature, relative humidity and soil temperature at different phenophases of winter maize would be useful in managing abiotic stresses on crop production.
ICSSC biennial progress report for 2020 :
Authors: Harris III John L , McCabe Steven L
Published: September 2023

Executive Order (EO) 13717: Establishing a Federal Earthquake Risk Management Standard establishes minimum seismic requirements for new and existing buildings that will be constructed, altered, leased, financed, or regulated by the Federal Government. These requirements help to enhance the seismic resilience of agencies by reducing risk to lives of building occupants and improving the probability of continued performance of buildings essential to agency mission following future earthquakes. The EO rescinds EO 12941: Seismic Safety of Existing Federally Owned or Leased Buildings and EO 12699: Seismic Safety of Federal and Federally Assisted or Regulated New Building Construction. The EO requires that agencies whose activities are covered by the order submit a biennial report to the Director of the Office of Management and Budget (OMB) and the Director of the National Institute of Standards and Technology (NIST) on their progress in implementing the order,
commencing two years from the date of the order. It is assumed that agencies that are covered by the EO participate on the Interagency Committee on Seismic Safety in Construction (ICSSC). NIST, as the lead agency of the National Earthquake Hazards Reduction Program (NEHRP) and thus Secretariat of the ICSSC, is required by the EO to summarize agency compliance of the order in the biennial NEHRP report to Congress. This report serves as the summary of agency progress in implementing the EO and covers the biennial reporting period of February 2, 2018 to February 1, 2020. Sixteen reports covering sixty-six agencies (department, agency, bureau, office, institute, etc.) were received by NIST for this reporting period.
Problem Based Learning Models to Numeracy Literacy Skills : A Study in Elementary School
Authors: Rahmah Indana Failur , Irianto Apri , Rachmadtullah Reza
Published: September 2023

: low numeracy literacy ability of students is a particular concern considering that in this 21st-century learning, students should have mastered this ability. The problem-based learning models is a learning model that presents a contextual problems so that students can be directly involved in the problem-solving process. Thus, this study aims to determine the effect of the problem-based learning model on the numeracy literacy skills of elementary school students. The research method used by researchers is a pre-experiment with one group pretest and posttest design using a quantitative approach. The sample in this study was eleven students in fifth grade at Waru Timur 2 Pamekasan Madura state elementary school. Data collection was carried out by administering tests (pretest and posttest) regarding students’ numeracy literacy abilities. Data were analyzed using descriptive statistics, prerequisite tests, and hypothesis testing with paired samples test. Based on the results of data processing, it is known that there is a significant influence of the use of problem-based learning models on students’ numeracy literacy skills. That is because learning is student-centered so that students can be directly involved in problem-solving
Rethinking Energy 2020-2030: 100% Solar, Wind, and Batteries is Just the Beginning
Authors: Dorr Adam , Seba Tony
Published: September 2023

We are on the cusp of the fastest, deepest, most profound disruption of the energy sector in over a century. Like most disruptions, this one is being driven by the convergence of several key technologies whose costs and capabilities have been improving on consistent and predictable trajectories – namely, solar photovoltaic power, wind power, and lithium-ion battery energy storage. Our analysis shows that 100% clean electricity from the combination of solar, wind, and batteries (SWB) is both physically possible and economically affordable across the entire continental United States as well as the overwhelming majority of other populated regions of the world by 2030. Adoption of SWB is growing exponentially worldwide and disruption is now inevitable because by 2030 they will offer the cheapest electricity option for most regions. Coal, gas, and nuclear power assets will become stranded during the 2020s, and no new investment in these technologies is rational from this point forward.
Rethinking Humanity: Five Foundational Sector Disruptions, the Lifecycle of Civilizations, and the Coming Age of Freedom
Authors: Arbib James , Seba Tony
Published: September 2023

During the 2020s, key technologies will converge to completely disrupt the five foundational sectors that underpin the global economy, and with them every major industry in the world today. In information, energy, food, transportation, and materials, costs will fall by a 10x or more, while production processes an order of magnitude more efficient will use 90% fewer natural resources with 10x-100x less waste. The knock-on effects for society will be as profound as the extraordinary possibilities that emerge. For the first time in history, we could overcome poverty easily. Access to all our basic needs could become a fundamental human right. But this is just one future outcome. The alternative could see our civilization collapse into a new dark age. Which path we take depends on the choices we make, starting today. The stakes could not be higher.
肾病综合征患者临床护理分析
Authors: 陈 卓卓 , 张 露
Published: September 2023

目的：分析肾病综合征的护理方案与效果。方法：收集2022年1月 -2023年1月接诊的肾病综合征患者60例，为所选患者提供常规、饮食以及心理护理，对于护理工作中的不足之处进行改进，对比60例患者护理前后的临床效果。结果：60例患者接受护理措施之后全部存在不同程度好转，和护理之前对比，患者24小时尿蛋白定量、尿素氮、血清白蛋白、肌酐以及总蛋白水平出现显著改善，对比存在显著统计学差异。结论：应用有效的护理方案到肾病综合征中，可以提高护理效果，促进肾病综合征患者身体的恢复。
社区视角下老年高血压患者服药依从性与自我感知老化的分析
Authors: 赵 东旭
Published: September 2023

目的：了解老年高血压患者服药依从性的现况和影响因素，探讨其与自我感知老化的相关性，以期从自我感知老化层面改善服药依从性水平，从而有助于社区卫生服务机构对患者进行精细化管理。方法：采用分层抽样法选取2021年1月到12月某社区的老年高血压患者500例为研究对象，剔除2份无效问卷，最终498例患者纳入研究。通过服药依从性量表、自我感知老化问卷进行评估，采用相关性分析及 logistic回归分析筛选出高血压患者服药依从性的相关影响因素。结果：498例患者中，服药依从性水平偏低，总得分为（4.60±1.56）分，依从性良好者占41%，不良者占59%；周期时间性、积极控制、积极结果维度与服药依从性呈正相关，均 P＜0.05；多因素分析结果显示，高中、大专及以上学历，非独居，积极控制和积极结果将会提高患者的服药依从性水平，具有保护作用，OR＜1，P＜0.05。结论：社区老年高血压患者的服药依从性处于较低水平，医护人员应该以积极控制、积极结果维度为切入点，针对性地制定出有利于提高患者自我感知老化水平的干预措施，同时纠正其消极的老化感知，进而提高服药依从性。
剖宫产术后应用优质护理缓解疼痛的临床观察
Authors: 张 力
Published: September 2023

目的：研究分析剖宫产术后应用优质护理缓解疼痛的临床观察。方法：选择该院2022年3月至2023年3月救治的80例剖宫产产妇；将孕产妇的病号写入球中并投入箱内，采取盲盒抽取法将其分为 A组和 B组。对照组孕产妇应用基础护理干预，A组孕产妇在 B组基础应用优质护理干预。密切观察孕产妇身体恢复效果，采取针对性评分表，依据真实数据，统计分析其疼痛程度和治疗疗效；结果：采取疼痛评分尺（NRS）评分显示：实验组疼痛程度明显低于对照组，组间差异有统计学意义（P＜0.05）。采取《护理疗效评分表》显示：实验组护理有效率（90%）明显高于 B组（67.5%），组间差异有统计学意义（P＜0.05）。结论：剖宫产术后实施优质护理，可满足护理需求，缓解其疼痛程度，提高护理有效率，为其治疗护理提高保障，具有推广意义。
优质护理服务在手术室护理中的作用
Authors: 刘 彦玲
Published: September 2023

目的：探讨分析优质护理服务在手术室护理中的作用。方法：采集该院2022年5月至2023年5月诊治的60例手术室治疗患者，采取盲盒抽取法将其分为 A组和 B组；对 B组患者采取常规护理，对 A组患者采取优质护理，统计分析两组患者的护理满意度；结果：依据《护理满意度评分表》数据显示：A组患者的护理满意度为93.33%明显高于 B组患者的护理满意度70%，组间差异有统计学意义（P＜0.05）。结论：优质护理服务在手术室护理中具有良好的应用价值，可满足手术标准和患者的治疗需求，提高其护理满意度，为患者提供优质、舒适的护理服务，值得广泛推广。
The analysis of the Process of Counselling and Psychotherapy
Authors: Ezy Maulany Ledita , Mudjiran Mudjiran , Sukma Dina
Published: September 2023

Today, many of us humans do not realize the importance of socializing, needing other people, medication, and so on. The things mentioned earlier can happen partly because we humans sometimes think that we can and are capable, even though this perception is not always true. In the world of counseling, this is said to be a human being who is KES-T (Effective Daily Life Disturbed). Furthermore, to help people with KES-T, counseling and psychotherapy are professional services that can help people overcome them. If you look at it in general, the process of counseling and psychotherapy is not much different, but if you look more closely, the two have differences in terms of concepts and different objects of practice. The method used in this article is an analysis of literature studies in which the collection of information regarding the counseling and psychotherapy process comes from all types of reading sources, both direct and indirect written sources such as printed or electronic reading sources
Mirror buckling analysis of freestanding graphene membranes by coarse-grained molecular dynamics method
Authors: Xu Wen-Long , Kai Yue , Zhang Kai , Zheng Bai-Lin ,   School of Aerospace Engineering and Applied Mechanics, Tongji University, Shanghai 200092, China,   School of Mathematics, Physics and Statistics, Center of Intelligent Computing and Applied Statistics, Shanghai University of Engineering Science, Shanghai 201620, China
Published: September 2023

Up to now, the analysis has rarely been conducted of thermal-mechanical mirror buckling behavior of freestanding graphene membranes discovered in scan tunneling microscope experiments. One of the potential applications of the out-of-plane deformational behavior of graphene membranes is energy harvesting system. Whether in the experiments or for energy harvesting systems, the size of graphene membrane needs to be down to micron scale. According to previous researches, traditional molecular dynamics method is a suitable method to characterize nano-scale mirror buckling. However, owing to the limit of algorithm, when dealing with micro size model by molecular dynamics method, two problems arise: low computational efficiency and too long calculation time. Therefore, for analyzing the mirror buckling of micro size graphene membranes, the coarse-grained molecular dynamics method is utilized in this work. Graphene membranes with a fan-shaped cross section and various depth-span ratios are under mechanical or thermal loads. Effects of each factor on the mirror buckling are investigated. The calculations indicate that for graphene membranes with various depth-span ratios under mechanical load mirror buckling can be observed. And the critical loading increases with the depth-span ratio increasing. Under thermal load graphene membranes only with low depth-span ratios can undergo complete flipping phenomenon. For high depth-span ratio graphene, the center height decreases with temperature rising. However, it is hard to flip over completely. The understanding of the effects of various factors on the mirror buckling phenomenon of graphene membranes can provide theoretical guidance for designing the energy harvesting systems.
Mega-scale movie-fields in the mouse visuo-hippocampal network
Authors: Purandare Chinmay S. , Mehta Mayank R.
Published: September 2023

Natural experience often involves a continuous series of related images while the subject is immobile. How does the cortico-hippocampal circuit process this information? The hippocampus is crucial for episodic memory
1–3
, but most rodent single unit studies require spatial exploration
4–6
or active engagement
7
. Hence, we investigated neural responses to a silent, isoluminant, black and white movie in head-fixed mice without any task or locomotion demands, or rewards, from the Allen Brain Observatory. The activity of most neurons (97%, 6554/6785) in the thalamo-cortical visual areas was significantly modulated by the 30s long movie clip. Surprisingly, a third (33%, 3379/10263) of hippocampal –dentate gyrus, CA1 and subiculum– neurons showed movie-selectivity, with elevated firing in specific movie sub-segments, termed movie-fields. Movie-tuning remained intact when mice were immobile or ran spontaneously. On average, a tuned cell had more than 5 movie-fields in visual areas, but only 2 in hippocampal areas. The movie-field durations in all brain regions spanned an unprecedented 1000-fold range: from 0.02s to 20s, termed mega-scale coding. Yet, the total duration of all the movie-fields of a cell was comparable across neurons and brain regions. We hypothesize that hippocampal responses show greater continuous-sequence encoding than visual areas, as evidenced by fewer and broader movie-fields than in visual areas. Consistent with this hypothesis, repeated presentation of the movie images in a fixed, scrambled sequence virtually abolished hippocampal but not visual-cortical selectivity. The enhancement of continuous movie tuning compared to the scrambled sequence was eight-fold greater in hippocampal than visual areas, further supporting episodic-sequence encoding. Thus, all mouse-brain areas investigated encoded segments of the movie. Similar results are likely to hold in primates and humans. Hence, movies could provide a unified way to probe neural mechanisms of episodic information processing and memory, even in immobile subjects, across brain regions, and species.
RNA-binding deficient TDP-43 drives cognitive decline in a mouse model of TDP-43 proteinopathy
Authors: Necarsulmer Julie , Simon Jeremy , Evangelista Baggio , Chen Youjun , Tian Xu , Nafees Sara , Marquez Gonzalez Ariana , Jiang Huijun , Wang Ping , Ajit Deepa , Nikolova Viktoriya , Harper Kathryn , Ezzell Jennifer , Lin Feng-Chang , Beltran Adriana , Moy Sheryl , Cohen Todd
Published: September 2023

TDP-43 proteinopathies including frontotemporal lobar degeneration (FTLD) and amyotrophic lateral sclerosis (ALS) are neurodegenerative disorders characterized by aggregation and mislocalization of the nucleic-acid binding protein TDP-43 and subsequent neuronal dysfunction. Here, we developed endogenous models of sporadic TDP-43 proteinopathy based on the principle that disease-associated TDP-43 acetylation at lysine 145 (K145) alters TDP-43 conformation, impairs RNA-binding capacity, and induces downstream mis-regulation of target genes. Expression of acetylation-mimic TDP-43
K145Q
resulted in stress-induced nuclear TDP-43 foci and loss of TDP-43 function in primary mouse and human induced pluripotent stem cell (hiPSC)-derived cortical neurons. Mice harboring the TDP-43
K145Q
mutation recapitulated key hallmarks of FTLD, including progressive TDP-43 phosphorylation and insolubility, TDP-43 mis-localization, transcriptomic and splicing alterations, and cognitive dysfunction. Our study supports a model in which TDP-43 acetylation drives neuronal dysfunction and cognitive decline through aberrant splicing and transcription of critical genes that regulate synaptic plasticity and stress response signaling. The neurodegenerative cascade initiated by TDP-43 acetylation recapitulates many aspects of human FTLD and provides a new paradigm to further interrogate TDP-43 proteinopathies.
Human skeletal muscle organoids model fetal myogenesis and sustain uncommitted PAX7 myogenic progenitors
Authors: Mavrommatis Lampros , Jeong Hyun-Woo , Kindler Urs , Gomez-Giro Gemma , Kienitz Marie-Cécile , Stehling Martin , Psathaki Olympia E. , Zeuschner Dagmar , Gabriele Bixel M. , Han Dong , Morosan-Puopolo Gabriela , Gerovska Daniela , Yang Ji Hun , Kim Jeong Beom , Araúzo-Bravo Marcos J. , Schwamborn Jens C. , Hahn Stephan A. , Adams Ralf H. , Schöler Hans R. , Vorgerd Matthias , Brand-Saberi Beate , Zaehres Holm
Published: September 2023

In vitro culture systems that structurally model human myogenesis and promote PAX7
+
myogenic progenitor maturation have not been established. Here we report that human skeletal muscle organoids can be differentiated from induced pluripotent stem cell lines to contain paraxial mesoderm and neuromesodermal progenitors and develop into organized structures reassembling neural plate border and dermomyotome. Culture conditions instigate neural lineage arrest and promote fetal hypaxial myogenesis towards limb axial anatomical identity, with generation of sustainable uncommitted PAX7 myogenic progenitors and fibroadipogenic (PDGFRa+) progenitor populations equivalent to those from the second trimester of human gestation. Single cell comparison to human fetal and adult myogenic progenitors reveals distinct molecular signatures for non-dividing myogenic progenitors in activated (CD44
High
/CD98
+
/MYOD1
+
) and dormant (PAX7
High
/FBN1
High
/SPRY1
High
) states. Our approach provides a robust 3D in vitro developmental system for investigating muscle tissue morphogenesis and homeostasis.
Integrin β4 promotes DNA damage-related drug resistance in triple-negative breast cancer via TNFAIP2/IQGAP1/RAC1
Authors: Fang Huan , Ren Wenlong , Cui Qiuxia , Liang Huichun , Yang Chuanyu , Liu Wenjing , Wang Xinye , Liu Xue , Shi Yujie , Feng Jing , Chen Ceshi
Published: September 2023

Anti-tumor drug resistance is a challenge for human triple-negative breast cancer treatment. Our previous work demonstrated that TNFAIP2 activates RAC1 to promote triple-negative breast cancer cell proliferation and migration. However, the mechanism by which TNFAIP2 activates RAC1 is unknown. In this study, we found that TNFAIP2 interacts with IQGAP1 and Integrin β4. Integrin β4 activates RAC1 through TNFAIP2 and IQGAP1 and confers DNA damage-related drug resistance in triple-negative breast cancer. These results indicate that the Integrin β4/TNFAIP2/IQGAP1/RAC1 axis provides potential therapeutic targets to overcome DNA damage-related drug resistance in triple-negative breast cancer.
Effects of blood meal source and seasonality on reproductive traits of Culex quinquefasciatus (Diptera: Culicidae)
Authors: Rucci Kevin Alen , Barco Gabriel , Onorato Andrea , Beranek Mauricio , Pueta Mariana , Díaz Adrián
Published: September 2023

Host selection by mosquitoes is a keystone to understanding viral circulation and predicting future infection outbreaks.
Culex
mosquitoes frequently feed on birds during spring and early summer, shifting into mammals towards late summer and autumn. This host switch may be due to changes in mosquito fitness. The aim of this study was to assess if the interaction effect of blood meal source and seasonality may influence reproductive traits of
Culex quinquefasciatus
mosquitoes. For this purpose,
Cx. quinquefasciatus
mosquitoes were reared in simulated summer and autumn conditions and fed on two different hosts, chickens and mice, in a factorial design. Fecundity, fertility, and hatchability during two consecutive gonotrophic cycles were estimated. We found greater fecundity and fertility for mosquitoes fed upon birds than mammals. Fecundity and fertility did not vary between seasons for chicken-fed mosquitoes, whereas in autumn they decreased for mouse-fed mosquitoes. These traits decreased in the second gonotrophic cycle for mouse-fed mosquitoes, whereas they did not vary between cycles for chicken-fed mosquitoes. There was no statistically significant variation of hatchability among treatments. These results indicate a statistically significant interaction effect of blood meal source and seasonality on fecundity and fertility. However, the pattern was opposite in relation to our hypothesis, suggesting that further studies are needed to confirm and expand our knowledge about mosquito biology and its relationship with seasonal host use shifting.
Effects of blood meal source and seasonality on reproductive traits of Culex quinquefasciatus (Diptera: Culicidae)
Authors: Rucci Kevin Alen , Pueta Mariana , Díaz Adrián
Published: September 2023

Host selection by mosquitoes is a keystone to understand viral circulation and predict future infection outbreaks.
Culex
mosquitoes frequently feed on birds during spring and early summer, shifting into mammals towards late summer and autumn. This host switch may be produced by changes in mosquito fitness. The aim of this study was to assess if blood meal source and seasonality may influence reproductive traits of
Culex quinquefasciatus
mosquitoes. For this purpose,
Cx. quinquefasciatus
mosquitoes were reared in simulated summer and autumn conditions and fed with two different hosts, chickens and mice, in a factorial design. Fecundity, fertility and hatchability during two consecutive gonotrophic cycles were estimated. We found a greater fecundity and fertility for mosquitoes fed upon birds than mammals. Fecundity and fertility increased in autumn for chicken-fed mosquitoes, whereas they decreased for mouse-fed mosquitoes. These traits decreased in the second gonotrophic cycle for mouse-fed mosquitoes, whereas they did not vary between cycles for chicken-fed mosquitoes. Blood meal source had a significant effect on hatchability, finding the lowest hatchability in mouse-fed mosquitoes during the second gonotrophic cycle, whereas no differences were detected among the remaining treatments. These results indicate that fecundity and fertility are influenced by blood meal source and seasonality, increasing towards autumn for mosquitoes fed on birds and decreasing for mosquitoes fed on mammals. Hatchability seems also being influenced by blood meal source. Our results suggest that blood meal source and seasonality induce metabolic changes in the mosquito reproductive physiology which might affect host selection patterns.
An in silico testbed for fast and accurate MR labeling of orthopedic implants
Authors: Noetscher Gregory M , Serano Peter J , Horner Marc , Prokop Alexander , Hanson Jonathan , Fujimoto Kyoko , Brown James , Nazarian Ara , Ackerman Jerome , Makaroff Sergey N
Published: September 2023

One limitation on the ability to monitor health in older adults using magnetic resonance (MR) imaging is the presence of implants, where the prevalence of implantable devices (orthopedic, cardiac, neuromodulation) increases in the population, as does the pervasiveness of conditions requiring MRI studies for diagnosis (musculoskeletal diseases, infections, or cancer). The present study describes a novel multiphysics implant modeling testbed using the following approaches with two examples: (1) an in silico human model based on the widely available Visible Human Project (VHP) cryo-section dataset; (2) a finite element method (FEM) modeling software workbench from Ansys (Electronics Desktop/Mechanical) to model MR radio frequency (RF) coils and the temperature rise modeling in heterogeneous media. The in silico VHP-Female model (250 parts with an additional 40 components specifically characterizing embedded implants and resultant surrounding tissues) corresponds to a 60-year-old female with a body mass index of 36. The testbed includes the FEM-compatible in silico human model, an implant embedding procedure, a generic parameterizable MRI RF birdcage two-port coil model, a workflow for computing heat sources on the implant surface and in adjacent tissues, and a thermal FEM solver directly linked to the MR coil simulator to determine implant heating based on an MR imaging study protocol. The primary target is MR labeling of large orthopedic implants. The testbed has very recently been approved by the US Food and Drug Administration (FDA) as a medical device development tool for 1.5 T orthopedic implant examinations.
Txnip deletions and missense alleles prolong the survival of cones in a retinitis pigmentosa mouse model
Authors: Xue Yunlu , Cepko Constance L.
Published: September 2023

Retinitis pigmentosa (RP) is a prevalent inherited retinal degenerative disease worldwide, affecting 1 in 4,000 people. The disease is characterized by an initial loss of night vision followed by a loss of daylight and color vision. Many of the RP disease genes are expressed in the rod photoreceptors, the cell type that initiates dim light vision. Following loss of rods, the cone photoreceptors, which initiate daylight vision, also are affected and can die leading to total loss of vision. The reasons for loss of cone vision are not entirely clear, but appear to be due to loss of the rods. Previously we showed that overexpressing Txnip, an α-arrestin protein, in mouse models of RP using AAV gene therapy prolonged the survival of RP cones (Xue et al. 2021). At least part of the mechanism for cone survival was a switch in the fuel source, from glucose to lactate. In addition, the mitochondria of cones were both morphologically and functionally improved by delivery of Txnip. We have gone on to test several alleles of Txnip for the ability to prolong cone survival in
rd1
, a mouse model of RP. In addition, proteins that bind to Txnip and/or have homology to Txnip were tested. Five different deletions of Txnip were expressed in cones or the retinal pigmented epithelium (RPE). Here we show that the C-terminal half of Txnip (149-397aa) is sufficient to remove GLUT1 from the RPE cell surface, and improved
rd1
cone survival when expressed specifically in the RPE. Overexpressing Arrdc4, an α-arrestin that shares 60% similar protein sequence to Txnip, reduced
rd1
cone survival. Reduction of the expression of HSP90AB1, a protein that interacts with Txnip and regulates metabolism, improved the survival of
rd1
cones alone and was additive for cone survival when combined with Txnip. However, full length Txnip with a single amino acid change, C247S, remains the most highly efficacious form of the gene for cone rescue. The above observations suggest that only a subset of the hypothesized and known activities of Txnip play a role in promoting RP cone survival, and that the activities of Txnip in the RPE differ from those in cone photoreceptors.
The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP
Authors: Körner Maria , Meyer Susanne R , Marincola Gabriella , Kern Maximilian J , Grimm Clemens , Schuelein-Voelk Christina , Fischer Utz , Hofmann Kay , Buchberger Alexander
Published: September 2023

The ATPase p97 (also known as VCP, Cdc48) has crucial functions in a variety of important cellular processes such as protein quality control, organellar homeostasis, and DNA damage repair, and its de-regulation is linked to neuromuscular diseases and cancer. p97 is tightly controlled by numerous regulatory cofactors, but the full range and function of the p97–cofactor network is unknown. Here, we identify the hitherto uncharacterized FAM104 proteins as a conserved family of p97 interactors. The two human family members VCP nuclear cofactor family member 1 and 2 (VCF1/2) bind p97 directly via a novel, alpha-helical motif and associate with p97-UFD1-NPL4 and p97-UBXN2B complexes in cells. VCF1/2 localize to the nucleus and promote the nuclear import of p97. Loss of VCF1/2 results in reduced nuclear p97 levels, slow growth, and hypersensitivity to chemical inhibition of p97 in the absence and presence of DNA damage, suggesting that FAM104 proteins are critical regulators of nuclear p97 functions.
miR-200a-3p overexpression alleviates diabetic cardiomyopathy injury in mice by regulating autophagy through the FOXO3/Mst1/Sirt3/AMPK axis
Authors: You Penghua , Chen Haichao , Han Wenqi , Deng Jizhao
Published: September 2023

Objective

Hyperglycemia and insulin resistance or deficiency are characteristic features of diabetes. Diabetes is accompanied by cardiomyocyte hypertrophy, fibrosis and ventricular remodeling, and eventually heart failure. In this study, we established a diabetic cardiomyopathy (DCM) mouse model to explore the role and mechanism of miR-200a-3p in DCM.

Methods

We used db/db mice to simulate the animal model of DCM and the expression of miR-200a-3p was then examined by RT-qPCR. Tail vein injection of mice was done with rAAV-miR-200a-3p for 8 weeks, and cardiac function was assessed by cardiac ultrasound. The levels of myocardial tissue injury, fibrosis, inflammation, apoptosis and autophagy in mice were detected by histological staining, TUNEL and other molecular biological experiments.

Results

miR-200a-3p expression levels were significantly decreased in the myocardium of DCM mice. Diabetic mice developed cardiac dysfunction and presented pathological changes such as myocardial injury, myocardial interstitial fibrosis, cardiomyocyte apoptosis, autophagy, and inflammation. Overexpression of miR-200a-3p expression significantly ameliorated diabetes induced-cardiac dysfunction and myocardial injury, myocardial interstitial fibrosis, cardiomyocyte apoptosis, and inflammation, and enhanced autophagy. Mechanistically, miR-200a-3p interacted with FOXO3 to promote Mst1 expression and reduce Sirt3 and p-AMPK expression.

Conclusion

In type 2 diabetes, increased miR-200a-3p expression enhanced autophagy and participated in the pathogenic process of cardiomyopathy throug7 Mst1/Sirt3/AMPK axis regulation by its target gene FOXO3. This conclusion provides clues for the search of new gene targeted therapeutic approaches for diabetic cardiomyopathy.