bluuebunny/crossref_metadata_2025 huggingface dataset:
Microbial Structure and Energy Generation in Microbial Fuel Cells Powered with Waste Anaerobic Digestate
Authors: Nosek Dawid , Cydzik-Kwiatkowska Agnieszka
Published: September 2020

Development of economical and environment-friendly Microbial Fuel Cells (MFCs) technology should be associated with waste management. However, current knowledge regarding microbiological bases of electricity production from complex waste substrates is insufficient. In the following study, microbial composition and electricity generation were investigated in MFCs powered with waste volatile fatty acids (VFAs) from anaerobic digestion of primary sludge. Two anode sizes were tested, resulting in organic loading rates (OLRs) of 69.12 and 36.21 mg chemical oxygen demand (COD)/(g MLSS∙d) in MFC1 and MFC2, respectively. Time of MFC operation affected the microbial structure and the use of waste VFAs promoted microbial diversity. High abundance of Deftia sp. and Methanobacterium sp. characterized start-up period in MFCs. During stable operation, higher OLR in MFC1 favored growth of exoelectrogens from Rhodopseudomonas sp. (13.2%) resulting in a higher and more stable electricity production in comparison with MFC2. At a lower OLR in MFC2, the percentage of exoelectrogens in biomass decreased, while the abundance of genera Leucobacter, Frigoribacterium and Phenylobacterium increased. In turn, this efficiently decomposed complex organic substances, favoring high and stable COD removal (over 85%). Independent of the anode size, Clostridium sp. and exoelectrogens belonging to genera Desulfobulbus and Acinetobacter were abundant in MFCs powered with waste VFAs.
Heterogeneity of Electricity Consumption Patterns in Vulnerable Households
Authors: Trotta Gianluca , Gram-Hanssen Kirsten , Lykke Jørgensen Pernille
Published: September 2020

A key aspect of the design of specific tariff structures is to identify and characterize homogeneous electricity consumption profiles. Recent research in residential electricity demand has explored load profile segmentation via cluster analysis combined with descriptive data from the dwelling and occupants, which has partly explained electricity load patterns and their underlying drivers but has failed to investigate any consumption heterogeneity among similar households. Thus, the aim of this paper is to reverse this approach and investigate the extent that households with similar characteristics have different electricity consumption patterns. This study combines population-based register data with hourly electricity consumption data for a sample of 67 Danish households. First, a homogenous household group is selected based on several indicators that signal vulnerability. The specific group under investigation is single-person, older, low-income households in detached housing. Second, K-means clustering is used to identify similarities and differences in consumption patterns. The results indicate four distinct vulnerable household profiles characterized by different start and end times of peak and off-peak times, peak intensities, and overall consumption, which vary across seasons. These profiles are discussed concerning the performance of everyday practices and the design of demand-side management strategies targeted at vulnerable households.
Numerical Investigation of Injection-Induced Fracture Propagation in Brittle Rocks with Two Injection Wells by a Modified Fluid-Mechanical Coupling Model
Authors: Wang Song , Zhou Jian , Zhang Luqing , Han Zhenhua
Published: September 2020

Hydraulic fracturing is a key technical means for stimulating tight and low permeability reservoirs to improve the production, which is widely employed in the development of unconventional energy resources, including shale gas, shale oil, gas hydrate, and dry hot rock. Although significant progress has been made in the simulation of fracturing a single well using two-dimensional Particle Flow Code (PFC2D), the understanding of the multi-well hydraulic fracturing characteristics is still limited. Exploring the mechanisms of fluid-driven fracture initiation, propagation and interaction under multi-well fracturing conditions is of great theoretical significance for creating complex fracture networks in the reservoir. In this study, a series of two-well fracturing simulations by a modified fluid-mechanical coupling algorithm were conducted to systematically investigate the effects of injection sequence and well spacing on breakdown pressure, fracture propagation and stress shadow. The results show that both injection sequence and well spacing make little difference on breakdown pressure but have huge impacts on fracture propagation pressure. Especially under hydrostatic pressure conditions, simultaneous injection and small well spacing increase the pore pressure between two injection wells and reduce the effective stress of rock to achieve lower fracture propagation pressure. The injection sequence can change the propagation direction of hydraulic fractures. When the in-situ stress is hydrostatic pressure, simultaneous injection compels the fractures to deflect and tend to propagate horizontally, which promotes the formation of complex fracture networks between two injection wells. When the maximum in-situ stress is in the horizontal direction, asynchronous injection is more conducive to the parallel propagation of multiple hydraulic fractures. Nevertheless, excessively small or large well spacing reduces the number of fracture branches in fracture networks. In addition, the stress shadow effect is found to be sensitive to both injection sequence and well spacing.
Ablation-Dominated Arcs in CO2 Atmosphere—Part II: Molecule Emission and Absorption
Authors: Methling Ralf , Götte Nicolas , Uhrlandt Dirk
Published: September 2020

Molecule radiation can be used as a tool to study colder regions in switching arc plasmas like arc fringes in contact to walls and ranges around current zero (CZ). This is demonstrated in the present study for the first time for the case of ablation-dominated high-current arcs as key elements of self-blast circuit breakers. The arc in a model circuit breaker (MCB) in CO2 with and an arc in a long nozzle under ambient conditions with peak currents between 5 and 10 kA were studied by emission and absorption spectroscopy in the visible spectral range. The nozzle material was polytetrafluoroethylene (PTFE) in both cases. Imaging spectroscopy was carried out either with high-speed cameras or with intensified CCD cameras. A pulsed high-intensity Xe lamp was applied as a background radiator for the broad-band absorption spectroscopy. Emission of Swan bands from carbon dimers was observed at the edge of nozzles only or across the whole nozzle radius with highest intensity in the arc center, depending on current and nozzle geometry. Furthermore, absorption of C2 Swan bands and CuF bands were found with the arc plasma serving as background radiator. After CZ, only CuF was detected in absorption experiments.
Time Series Forecasting with Multi-Headed Attention-Based Deep Learning for Residential Energy Consumption
Authors: Bu Seok-Jun , Cho Sung-Bae
Published: September 2020

Predicting residential energy consumption is tantamount to forecasting a multivariate time series. A specific window for several sensor signals can induce various features extracted to forecast the energy consumption by using a prediction model. However, it is still a challenging task because of irregular patterns inside including hidden correlations between power attributes. In order to extract the complicated irregular energy patterns and selectively learn the spatiotemporal features to reduce the translational variance between energy attributes, we propose a deep learning model based on the multi-headed attention with the convolutional recurrent neural network. It exploits the attention scores calculated with softmax and dot product operation in the network to model the transient and impulsive nature of energy demand. Experiments with the dataset of University of California, Irvine (UCI) household electric power consumption consisting of a total 2,075,259 time-series show that the proposed model reduces the prediction error by 31.01% compared to the state-of-the-art deep learning model. Especially, the multi-headed attention improves the prediction performance even more by up to 27.91% than the single-attention.
Development of Brushless Claw Pole Electrical Excitation and Combined Permanent Magnet Hybrid Excitation Generator for Vehicles
Authors: Geng Huihui , Zhang Xueyi , Zhang Yufeng , Hu Wenjing , Lei Yulong , Xu Xiaoming , Wang Aichuan , Wang Shanjian , Shi Liwei
Published: September 2020

Aiming at the problems of large excitation loss and low power generation efficiency of silicon rectifier generators and the unstable output voltage of permanent magnet (PM) generators, a hybrid excitation generator (HEG) with suspended brushless claw pole electrical excitation rotor (EER) and combined magnetic pole PM rotor is proposed in the present work. With only one fractional slot winding stator, the generator adopts PM field as the main magnetic field and electrical excitation field as the auxiliary magnetic field, which not only retains the advantages of high efficiency of PM generators but also effectively reduces excitation consumption. The main structure parameters and the design method were analyzed, and a simulation analysis of no-load magnetic field distribution and flux regulation ability was carried out using finite element software to verify the rationality of the hybrid excitation parallel magnetic circuit design. Moreover, the no-load, load, regulation, and voltage regulation characteristics of the designed generator were tested, and the results show that the designed generator has a wide range of voltage regulation, which can ensure stable output voltage under variable speed and load conditions.
Industrial Energy Transitions and the Dynamics of Innovation Systems: The Swedish Pulp and Paper Industry, 1970–2010
Authors: Söderholm Kristina , Söderholm Patrik
Published: September 2020

This article provides a sectoral innovation system perspective of the development of energy efficient and clean process technologies in the Swedish pulp and paper industry. Specifically, the analysis elaborates the importance of knowledge development, actor networks, and institutions (including policy) for progressing and diffusing novel technologies related to energy use. The empirical analysis also sheds light on how significant changes in the sectoral innovation system have influenced the relevant research, development and demonstration activities in the Swedish pulp and paper industry over the period 1970–2010. The results are based on various sources—e.g., industry magazines, reports from industrial consultants and associations, minutes from meetings—and illustrate the importance of well-functioning innovation systems for successful technological development and diffusion processes. They display, in particular, the importance of joint, industry-wide R&D activities, trust-based state—industry relationships, government R&D expenditures, and intense information sharing. One important implication is that the role of policy stretches beyond the funding of basic R&D. Policy also involves measures that strengthen existing actor networks, build competence, and secure the existence of research institutes that provide a bridge between basic knowledge generation (at the universities) on the one hand, and industrial application on the other.
Carbon Cycling in the World’s Mangrove Ecosystems Revisited: Significance of Non-Steady State Diagenesis and Subsurface Linkages between the Forest Floor and the Coastal Ocean
Authors: Alongi Daniel M.
Published: September 2020

Carbon cycling within the deep mangrove forest floor is unique compared to other marine ecosystems with organic carbon input, mineralization, burial, and advective and groundwater export pathways being in non-steady-state, often oscillating in synchrony with tides, plant uptake, and release/uptake via roots and other edaphic factors in a highly dynamic and harsh environment. Rates of soil organic carbon (CORG) mineralization and belowground CORG stocks are high, with rapid diagenesis throughout the deep (>1 m) soil horizon. Pocketed with cracks, fissures, extensive roots, burrows, tubes, and drainage channels through which tidal waters percolate and drain, the forest floor sustains non-steady-state diagenesis of the soil CORG, in which decomposition processes at the soil surface are distinct from those in deeper soils. Aerobic respiration occurs within the upper 2 mm of the soil surface and within biogenic structures. On average, carbon respiration across the surface soil-air/water interface (104 mmol C m−2 d−1) equates to only 25% of the total carbon mineralized within the entire soil horizon, as nearly all respired carbon (569 mmol C m−2 d−1) is released in a dissolved form via advective porewater exchange and/or lateral transport and subsurface tidal pumping to adjacent tidal waters. A carbon budget for the world’s mangrove ecosystems indicates that subsurface respiration is the second-largest respiratory flux after canopy respiration. Dissolved carbon release is sufficient to oversaturate water-column pCO2, causing tropical coastal waters to be a source of CO2 to the atmosphere. Mangrove dissolved inorganic carbon (DIC) discharge contributes nearly 60% of DIC and 27% of dissolved organic carbon (DOC) discharge from the world’s low latitude rivers to the tropical coastal ocean. Mangroves inhabit only 0.3% of the global coastal ocean area but contribute 55% of air-sea exchange, 14% of CORG burial, 28% of DIC export, and 13% of DOC + particulate organic matter (POC) export from the world’s coastal wetlands and estuaries to the atmosphere and global coastal ocean.
The Relationship between Workplace Violence and Innovative Work Behavior: The Mediating Roles of Employee Wellbeing
Authors: Zhou Xiang , Rasool Samma Faiz , Ma Dawei
Published: September 2020

It has been contended that violence is prevalent in the workplace, and there has been increasing research interest into its potential effects. Human interactions at workplaces are apparent. However, the interactions among humans may have positive or negative dimensions. Usually, the positive or negative interactions between workers lead to different outcomes. Sometimes, they lead to a productive working environment; however, in some cases, they lead to toxicity among workers. In this study, we investigate the impact of workplace violence (WV) on innovative work behavior (IWB). Specifically, it examines the impact of the three dimensions of WV, namely, harassment, mobbing, and sabotage. Moreover, employees’ wellbeing mediates the relationship between WV (harassment, mobbing, and sabotage) and IWB. A questionnaire survey approach was used in this study. The target population were the workers of SMEs entrepreneurs located in Guangdong Province (China). The results confirm that, in the direct relationship, WV (harassment, mobbing, and sabotage) has a negative relationship with innovative IWB. Moreover, results also confirm that employee wellbeing is mediated between WV (harassment, mobbing, and sabotage) and IWB. Therefore, the empirical results of this paper identify that workplace violence reduces employees’ innovative work behavior by reducing their subjective and eudemonic wellbeing, which further broadens the perspective of IWB’s motivation analysis. Practical implications for small and medium enterprise organizations have also been discussed in this paper.
Improvement of Periodontal Parameters with the Sole Use of Free Gingival Grafts in Orthodontic Patients: Correlation with Periodontal Indices. A 15-Month Clinical Study
Authors: Orsini Marco , Benlloch Dunia , Aranda Macera Juan José , Flores Karina , Ríos-Santos José-Vicente , Pedruelo Francisco Javier , Ríos-Carrasco Blanca , di Cesare Massimo
Published: September 2020

The aim of this study was to evaluate the changes in periodontal parameters solely using free gingival grafts during orthodontic treatment without any oral hygiene re-enforcement. Methods: A total of 19 patients underwent periodontal examination before orthodontic treatment. Patients received oral hygiene instruction and professional hygiene therapy. Where needed; full periodontal treatment was completed. Only periodontally stable patients were included in the study. Periodontal indices and keratinized tissue were recorded at time 0 (T0) (delivery of orthodontic appliances), and at three months (T1) during orthodontic therapy; when surgery was performed. At T1; orthodontically treated sites with minimum keratinized tissue (≤1 mm) received a free gingival graft to enhance the band of keratinized tissue. At three months after surgery (T2), new measurements were recorded. The orthodontics-treated sites after three months (T1) were used as control. The same sites were used as a test three months after mucogingival correction (T2). Between T1 and T2; orthodontics was suspended; no professional oral hygiene was performed; and no additional oral hygiene instructions were given to the patient. No oral hygiene procedures were administered for 15 months (T3), when the final recordings were taken. Results: The results showed that there was a worsening of gingival index (GI) and plaque index (PI) of the treated sites between T0 and T1 during initial orthodontics treatment; whereas there was an improvement of the gingival inflammation at T2 when compared with T1. At T2; there was also a statistically significant improvement in GI and PI compared with T0. A T3 improvement in periodontal parameters was sustained. A non-parametric test (Wilcoxon signed-rank test) was used for statistical analysis. Conclusions: Augmentation of the width of keratinized gingiva; as the sole treatment; favors the improvement of GI and PI during orthodontic therapy.
Kinetics of IgG Antibodies in Previous Cases of Dengue Fever—A Longitudinal Serological Survey
Authors: Wu Qilin , Jing Qinlong , Wang Xiujuan , Yang Lili , Li Yilan , Chen Zongqiu , Ma Mengmeng , Yang Zhicong
Published: September 2020

Guangzhou is believed to be the most important epicenter of dengue outbreaks in southern China. In this study, a longitudinal serological investigation of previous cases of dengue fever in Guangzhou was conducted to explore the persistence of IgG antibodies and related factors affecting the changes of antibody level. We recruited 70 dengue virus type 1 (DENV-1) primary infection cases at two years post infection for serological investigation and conducted a second follow-up in the 5th year of prognosis. An enzyme-linked immunosorbent assay (ELISA) for DENV IgG antibody was examined in all study subjects. Potential factors associated with the concentration of serum total IgG antibody were determined by the generalized estimation equation (GEE). No significant difference in serum total IgG antibody positive rate between two follow-ups was observed (χ2 = 3.066, p = 0.080). However, there was a significant difference in the concentration of serum total IgG antibody between the two follow-ups (Z = 7.154, p < 0.001). The GEE showed that the antibody level in the five-year prognosis was mainly affected by the antibody level in the two-year prognosis (OR: 1.007, 95%CI: 1.005–1.009). In conclusion, the serum IgG antibodies of previous dengue fever cases can persist for a long time.
Mapping the Patient’s Journey in Healthcare through Process Mining
Authors: Arias Michael , Rojas Eric , Aguirre Santiago , Cornejo Felipe , Munoz-Gama Jorge , Sepúlveda Marcos , Capurro Daniel
Published: September 2020

Nowadays, assessing and improving customer experience has become a priority, and has emerged as a key differentiator for business and organizations worldwide. A customer journey (CJ) is a strategic tool, a map of the steps customers follow when engaging with a company or organization to obtain a product or service. The increase of the need to obtain knowledge about customers’ perceptions and feelings when interacting with participants, touchpoints, and channels through different stages of the customer life cycle. This study aims to describe the application of process mining techniques in healthcare as a tool to asses customer journeys. The appropriateness of the approach presented is illustrated through a case study of a key healthcare process. Results depict how a healthcare process can be mapped through the CJ components, and its analysis can serve to understand and improve the patient’s experience.
Impact of Social Support and Social Trust on Public Viral Risk Response: A COVID-19 Survey Study
Authors: Song Eugene , Yoo Hyun Jung
Published: September 2020

Public health has been under continuous threat worldwide in recent years. This study examined the impact of social support and social trust on the activities and efficacy of the public’s risk response in the case of COVID-19. We conducted an online survey over eight days with 620 Korean adult participants. Data were analyzed using structural equation modelling and K-means cluster analysis. Our results showed that public support had a positive impact on response efficacy, while response efficacy had a positive impact on sanitation, distancing, and purchasing activities. In addition, social support positively moderated the impact of public and individual support on response efficacy, while response efficacy negatively moderated the impact on sanitation activities. These results suggest that, first, amid viral risk, governments should proactively supply tools and information for infection-prevention, and deliver messages that encourage and support infection-prevention activities among the public. Second, when viral risk occurs, governments, along with all other members of society, must engage in aggressive risk response measures. Third, there is a need for risk communication that further emphasizes the importance of personal sanitation activities in the face of viral risk.
The Value of Case Reports in Systematic Reviews from Rare Diseases. The Example of Enzyme Replacement Therapy (ERT) in Patients with Mucopolysaccharidosis Type II (MPS-II)
Authors: Sampayo-Cordero Miguel , Miguel-Huguet Bernat , Malfettone Andrea , Pérez-García José Manuel , Llombart-Cussac Antonio , Cortés Javier , Pardo Almudena , Pérez-López Jordi
Published: September 2020

Background: Case reports are usually excluded from systematic reviews. Patients with rare diseases are more dependent on novel individualized strategies than patients with common diseases. We reviewed and summarized the novelties reported by case reports in mucopolysaccharidosis type II (MPS-II) patients treated with enzyme replacement therapy (ERT). Methods: We selected the case reports included in a previous meta-analysis of patients with MPS-II treated with ERT. Later clinical studies evaluating the same topic of those case reports were reported. Our primary aim was to summarize novelties reported in previous case reports. Secondary objectives analyzed the number of novelties evaluated in subsequent clinical studies and the time elapsed between the publication of the case report to the publication of the clinical study. Results: We identified 11 innovative proposals in case reports that had not been previously considered in clinical studies. Only two (18.2%) were analyzed in subsequent nonrandomized cohort studies. The other nine novelties (81.8%) were analyzed in later case reports (five) or were not included in ulterior studies (four) after more than five years from their first publication. Conclusions: Case reports should be included in systematic reviews of rare disease to obtain a comprehensive summary of the state of research and offer valuable information for healthcare practitioners.
Preference for Aggressive End-of-Life Care among Advanced Cancer Patients in Wuhan, China: A Cross-Sectional Study
Authors: Liao Jing , Wu Bei , Mao Jing , Ni Ping
Published: September 2020

Life-sustaining treatments (LSTs) and end-of-life (EOL) care’s goal for prolonging one’s life are defined as aggressive EOL care among critically ill patients. They have limited effects and add unnecessary financial burden to advanced cancer patients. A questionnaire survey was conducted to collect information on demographics, disease conditions, preference for LSTs, and goal of EOL care among advanced cancer patients of comprehensive grade-A tertiary hospitals in Wuhan, mainland China. Most patients preferred to accept LSTs when they were in a critical condition, including cardiopulmonary resuscitation (89.9%), mechanical ventilation support (85.7%), nasogastric tube feeding (84.1%), blood transfusion (89.8%), general surgery (87.5%), and hemodialysis (85.8%). Most (88%) preferred prolonging life as the goal of EOL care. Logistic regression showed common influencing factors were participants who completed junior high/high school or below and were financially adequate had higher reference for aggressive EOL care. Patients whose physician had accurately disclosed prognosis; however, showed a decrease trend for aggressive EOL care. Most advanced cancer patients preferred to accept aggressive EOL care. Discussions about prognosis disclosure among physicians and patients should be improved. Education about LSTs’ limitations and comfort-oriented care’s benefits should be promoted among the advanced cancer patients in mainland China.
Positive and Negative Impacts of Caring among Adolescents Caring for Grandparents. Results from an Online Survey in Six European Countries and Implications for Future Research, Policy and Practice
Authors: Santini Sara , Socci Marco , D’Amen Barbara , Di Rosa Mirko , Casu Giulia , Hlebec Valentina , Lewis Feylyn , Leu Agnes , Hoefman Renske , Brolin Rosita , Magnusson Lennart , Hanson Elizabeth
Published: September 2020

Although up to 8% of European youngsters carry out high-intensity care for a family member, adolescent young carers (AYCs), especially those caring for their grandparents (GrPs), remain an under-researched group. This study aimed at addressing the current knowledge gap by carrying out an online survey in Italy, the Netherlands, Slovenia, Sweden, Switzerland, and the United Kingdom. The analysis included a final sample of 817 AYCs aged 15–17 years old. AYCs of grandparents (GrPs) were compared to AYCs of other care recipients (OCRs), in order to identify any difference in positive and negative caregiving outcomes and exposure factors between the two groups. Linear or logistic regression models were built, and multivariate analyses were repeated, including a fixed effect on the country variable. AYCs of GrPs experienced more positive caregiving outcomes than AYCs of OCRs across all six countries. Being female or non-binary, and having a migration background, were associated with more negative outcomes, regardless of the relationship with the care recipient. Further research on intergenerational caregiving outcomes is recommended for shaping measures and policies, which preserve the intergenerational emotional bonds, whilst protecting AYCs from inappropriate responsibilities, undermining their mental health and well-being.
Prevalence of Anxiety in Medical Students during the COVID-19 Pandemic: A Rapid Systematic Review with Meta-Analysis
Authors: Lasheras Isabel , Gracia-García Patricia , Lipnicki Darren , Bueno-Notivol Juan , López-Antón Raúl , de la Cámara Concepción , Lobo Antonio , Santabárbara Javier
Published: September 2020

The novel coronavirus disease (COVID-19) pandemic has brought a great deal of pressure for medical students, who typically show elevated anxiety rates. Our aim is to investigate the prevalence of anxiety in medical students during this pandemic. This systematic review and mini meta-analysis has been conducted following the PRISMA guidelines. Two researchers independently searched PubMed on 26 August 2020 for cross-sectional studies on medical students during the COVID-19 outbreak, with no language restrictions applied. We then performed a manual search to detect other potentially eligible investigations. To the 1361 records retrieved in the initial search, 4 more were added by manual search on medRxiv. Finally, eight studies were finally included for qualitative and quantitative analysis, which yielded an estimated prevalence of anxiety of 28% (95% CI: 22–34%), with significant heterogeneity between studies. The prevalence of anxiety in medical students is similar to that prior to the pandemic but correlates with several specific COVID-related stressors. While some preventive and risk factors have been previously identified in a non-pandemic context, knowledge and cognitions on COVID-19 transmission, treatment, prognosis and prevention negatively correlate with anxiety, emerging as a key preventive factor that may provide a rationale for why the levels of anxiety have remained stable in medical students during the pandemic while increasing in their non-medical peers and the general population. Other reasons for the invariability of anxiety rates in this population are discussed. A major limitation of our review is that Chinese students comprised 89% the total sample, which could compromise the external validity of our work
Small Heat Shock Proteins in Cancers: Functions and Therapeutic Potential for Cancer Therapy
Authors: Xiong Jixian , Li Yuting , Tan Xiangyu , Fu Li
Published: September 2020

Small heat shock proteins (sHSPs) are ubiquitous ATP-independent chaperones that play essential roles in response to cellular stresses and protein homeostasis. Investigations of sHSPs reveal that sHSPs are ubiquitously expressed in numerous types of tumors, and their expression is closely associated with cancer progression. sHSPs have been suggested to control a diverse range of cancer functions, including tumorigenesis, cell growth, apoptosis, metastasis, and chemoresistance, as well as regulation of cancer stem cell properties. Recent advances in the field indicate that some sHSPs have been validated as a powerful target in cancer therapy. In this review, we present and highlight current understanding, recent progress, and future challenges of sHSPs in cancer development and therapy.
Applying Single-Cell Analysis to Gonadogenesis and DSDs (Disorders/Differences of Sex Development)
Authors: Estermann Martin A. , Smith Craig A.
Published: September 2020

The gonads are unique among the body’s organs in having a developmental choice: testis or ovary formation. Gonadal sex differentiation involves common progenitor cells that form either Sertoli and Leydig cells in the testis or granulosa and thecal cells in the ovary. Single-cell analysis is now shedding new light on how these cell lineages are specified and how they interact with the germline. Such studies are also providing new information on gonadal maturation, ageing and the somatic-germ cell niche. Furthermore, they have the potential to improve our understanding and diagnosis of Disorders/Differences of Sex Development (DSDs). DSDs occur when chromosomal, gonadal or anatomical sex are atypical. Despite major advances in recent years, most cases of DSD still cannot be explained at the molecular level. This presents a major pediatric concern. The emergence of single-cell genomics and transcriptomics now presents a novel avenue for DSD analysis, for both diagnosis and for understanding the molecular genetic etiology. Such -omics datasets have the potential to enhance our understanding of the cellular origins and pathogenesis of DSDs, as well as infertility and gonadal diseases such as cancer.
Colon Transcriptomics Reveals Sex-Dependent Metabolic Signatures in Response to 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine Treatment in C57BL/6N Mice
Authors: Pan Jeong Hoon , Cicalo Cara , Le Brandy , Jeon Suwon , Kim Sangyub , Hwang Kyung A. , Kong Byungwhi , Lee Jin Hyup , Kim Jae Kyeom
Published: September 2020

Diets high in red meats, particularly meats cooked at high temperature, increase the risk of colon cancer due to a production of heterocyclic aromatic amines (HAAs). Of the identified HAAs, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is the most mass abundant colon carcinogen in charred meat or fish. Here, we comprehensively examined sex-dependent colon transcriptome signatures in response to PhIP treatment to identify biological discrepancies. Eight-week-old male and female C57BL/6N mice were intraperitoneally injected with PhIP (10 mg/kg of body weight) and colon tissues were harvested 24 h after PhIP injection, followed by colon transcriptomics analysis. A list of differentially expressed genes (DEGs) was utilized for computational bioinformatic analyses. Specifically, overrepresentation test using the Protein Analysis Through Evolutionary Relationships tool was carried out to annotate sex-dependent changes in transcriptome signatures after PhIP treatment. Additionally, the most significantly affected canonical pathways by PhIP treatment were predicted using the Ingenuity Pathway Analysis. As results, male and female mice presented different metabolic signatures in the colon transcriptome. In the male mice, oxidative phosphorylation in the mitochondrial respiratory chain was the pathway impacted the most; this might be due to a shortage of ATP for DNA repair. On the other hand, the female mice showed concurrent activation of lipolysis and adipogenesis. The present study provides the foundational information for future studies of PhIP effects on underlying sex-dependent mechanisms.
Chitinase Gene Positively Regulates Hypersensitive and Defense Responses of Pepper to Colletotrichum acutatum Infection
Authors: Ali Muhammad , Li Quan-Hui , Zou Tao , Wei Ai-Min , Gombojab Ganbat , Lu Gang , Gong Zhen-Hui
Published: September 2020

Anthracnose caused by Colletotrichum acutatum is one of the most devastating fungal diseases of pepper (Capsicum annuum L.). The utilization of chitin-binding proteins or chitinase genes is the best option to control this disease. A chitin-binding domain (CBD) has been shown to be crucial for the innate immunity of plants and activates the hypersensitive response (HR). The CaChiIII7 chitinase gene has been identified and isolated from pepper plants. CaChiIII7 has repeated CBDs that encode a chitinase enzyme that is transcriptionally stimulated by C. acutatum infection. The knockdown of CaChiIII7 in pepper plants confers increased hypersensitivity to C. acutatum, resulting in its proliferation in infected leaves and an attenuation of the defense response genes CaPR1, CaPR5, and SAR8.2 in the CaChiIII7-silenced pepper plants. Additionally, H2O2 accumulation, conductivity, proline biosynthesis, and root activity were distinctly reduced in CaChiIII7-silenced plants. Subcellular localization analyses indicated that the CaChiIII7 protein is located in the plasma membrane and cytoplasm of plant cells. The transient expression of CaChiIII7 increases the basal resistance to C. acutatum by significantly expressing several defense response genes and the HR in pepper leaves, accompanied by an induction of H2O2 biosynthesis. These findings demonstrate that CaChiIII7 plays a prominent role in plant defense in response to pathogen infection.
Evaluating the Genotoxic and Cytotoxic Effects of Thymidine Analogs, 5-Ethynyl-2′-Deoxyuridine and 5-Bromo-2′-Deoxyurdine to Mammalian Cells
Authors: Haskins Jeremy S. , Su Cathy , Maeda Junko , Walsh Kade D. , Haskins Alexis H. , Allum Allison J. , Froning Coral E. , Kato Takamitsu A.
Published: September 2020

BrdU (bromodeoxyuridine) and EdU (ethynyldeoxyuridine) have been largely utilized as the means of monitoring DNA replication and cellular division. Although BrdU induces gene and chromosomal mutations and induces sensitization to photons, EdU‘s effects have not been extensively studied yet. Therefore, we investigated EdU’s potential cytotoxic and mutagenic effects and its related underlying mechanisms when administered to Chinese hamster ovary (CHO) wild type and DNA repair-deficient cells. EdU treatment displayed a higher cytotoxicity and genotoxicity than BrdU treatment. Cells with defective homologous recombination repair displayed a greater growth delay and severe inhibition of clonogenicity with EdU compared to wild type and other DNA repair-deficient cells. Inductions of sister chromatid exchange and hypoxanthine phosphorybosyl transferase (HPRT) mutation were observed in EdU-incorporated cells as well. Interestingly, on the other hand, EdU did not induce sensitization to photons to the same degree as BrdU. Our results demonstrate that elevated concentrations (similar to manufacturers suggested concentration; >5–10 μM) of EdU treatment were toxic to the cell cultures, particularly in cells with a defect in homologous recombination repair. Therefore, EdU should be administered with additional precautions.
Superparamagnetic Iron Oxide Nanoparticles and Essential Oils: A New Tool for Biological Applications
Authors: Miguel Maria Graça , Lourenço João Paulo , Faleiro Maria Leonor
Published: September 2020

Essential oils are complex mixtures of volatile compounds with diverse biological properties. Antimicrobial activity has been attributed to the essential oils as well as their capacity to prevent pathogenic microorganisms from forming biofilms. The search of compounds or methodologies with this capacity is of great importance due to the fact that the adherence of these pathogenic microorganisms to surfaces largely contributes to antibiotic resistance. Superparamagnetic iron oxide nanoparticles have been assayed for diverse biomedical applications due to their biocompatibility and low toxicity. Several methods have been developed in order to obtain functionalized magnetite nanoparticles with adequate size, shape, size distribution, surface, and magnetic properties for medical applications. Essential oils have been evaluated as modifiers of the surface magnetite nanoparticles for improving their stabilization but particularly to prevent the growth of microorganisms. This review aims to provide an overview on the current knowledge about the use of superparamagnetic iron oxide nanoparticles and essential oils on the prevention of microbial adherence and consequent biofilm formation with the goal of being applied on the surface of medical devices. Some limitations found in the studies are discussed.
Roles of Specialized Pro-Resolving Lipid Mediators in Autophagy and Inflammation
Authors: Recchiuti Antonio , Isopi Elisa , Romano Mario , Mattoscio Domenico
Published: September 2020

Autophagy is a catabolic pathway that accounts for degradation and recycling of cellular components to extend cell survival under stress conditions. In addition to this prominent role, recent evidence indicates that autophagy is crucially involved in the regulation of the inflammatory response, a tightly controlled process aimed at clearing the inflammatory stimulus and restoring tissue homeostasis. To be efficient and beneficial to the host, inflammation should be controlled by a resolution program, since uncontrolled inflammation is the underlying cause of many pathologies. Resolution of inflammation is an active process mediated by a variety of mediators, including the so-called specialized pro-resolving lipid mediators (SPMs), a family of endogenous lipid autacoids known to regulate leukocyte infiltration and activities, and counterbalance cytokine production. Recently, regulation of autophagic mechanisms by these mediators has emerged, uncovering unappreciated connections between inflammation resolution and autophagy. Here, we summarize mechanisms of autophagy and resolution, focusing on the contribution of autophagy in sustaining paradigmatic examples of chronic inflammatory disorders. Then, we discuss the evidence that SPMs can restore dysregulated autophagy, hypothesizing that resolution of inflammation could represent an innovative approach to modulate autophagy and its impact on the inflammatory response.
Domain Organization in Plant Blue-Light Receptor Phototropin2 of Arabidopsis thaliana Studied by Small-Angle X-ray Scattering
Authors: Nakasako Masayoshi , Oide Mao , Takayama Yuki , Oroguchi Tomotaka , Okajima Koji
Published: September 2020

Phototropin2 (phot2) is a blue-light (BL) receptor protein that regulates the BL-dependent activities of plants for efficient photosynthesis. Phot2 is composed of two light-oxygen-voltage sensing domains (LOV1 and LOV2) to absorb BL, and a kinase domain. Photo-activated LOV domains, especially LOV2, play a major role in photo-dependent increase in the phosphorylation activity of the kinase domain. The atomic details of the overall structure of phot2 and the intramolecular mechanism to convert BL energy to a phosphorylation signal remain unknown. We performed structural studies on the LOV fragments LOV1, LOV2, LOV2-linker, and LOV2-kinase, and full-length phot2, using small-angle X-ray scattering (SAXS). The aim of the study was to understand structural changes under BL irradiation and discuss the molecular mechanism that enhance the phosphorylation activity under BL. SAXS is a suitable technique for visualizing molecular structures of proteins in solution at low resolution and is advantageous for monitoring their structural changes in the presence of external physical and/or chemical stimuli. Structural parameters and molecular models of the recombinant specimens were obtained from SAXS profiles in the dark, under BL irradiation, and after dark reversion. LOV1, LOV2, and LOV2-linker fragments displayed minimal structural changes. However, BL-induced rearrangements of functional domains were noted for LOV2-kinase and full-length phot2. Based on the molecular model together with the absorption measurements and biochemical assays, we discuss the intramolecular interactions and domain motions necessary for BL-enhanced phosphorylation activity of phot2.
A Peripheral CB1R Antagonist Increases Lipolysis, Oxygen Consumption Rate, and Markers of Beiging in 3T3-L1 Adipocytes Similar to RIM, Suggesting that Central Effects Can Be Avoided
Authors: Paszkiewicz Rebecca L. , Bergman Richard N. , Santos Roberta S. , Frank Aaron P. , Woolcott Orison O. , Iyer Malini S. , Stefanovski Darko , Clegg Deborah J. , Kabir Morvarid
Published: September 2020

With the increased prevalence of obesity and related co-morbidities, such as type 2 diabetes (T2D), worldwide, improvements in pharmacological treatments are necessary. The brain- and peripheral-cannabinoid receptor 1 (CB1R) antagonist rimonabant (RIM) has been shown to induce weight loss and improve glucose homeostasis. We have previously demonstrated that RIM promotes adipose tissue beiging and decreased adipocyte cell size, even during maintenance on a high-fat diet. Given the adverse side-effects of brain-penetrance with RIM, in this study we aimed to determine the site of action for a non-brain-penetrating CB1R antagonist AM6545. By using in vitro assays, we demonstrated the direct effects of this non-brain-penetrating CB1R antagonist on cultured adipocytes. Specifically, we showed, for the first time, that AM6545 significantly increases markers of adipose tissue beiging, mitochondrial biogenesis, and lipolysis in 3T3-L1 adipocytes. In addition, the oxygen consumption rate (OCR), consisting of baseline respiratory rate, proton leak, maximal respiratory capacity, and ATP synthase activity, was greater for cells exposed to AM6545, demonstrating greater mitochondrial uncoupling. Using a lipolysis inhibitor during real-time OCR measurements, we determined that the impact of CB1R antagonism on adipocytes is driven by increased lipolysis. Thus, our data suggest the direct role of CB1R antagonism on adipocytes does not require brain penetrance, supporting the importance of focus on peripheral CB1R antagonism pharmacology for reducing the incidence of obesity and T2D.
Fusion of Angle Measurements from Hull Mounted and Towed Array Sensors
Authors: Jahan Kausar , Sanagapallea Koteswara Rao
Published: September 2020

Two sensor arrays, hull-mounted array, and towed array sensors are considered for bearings-only tracking. An algorithm is designed to combine the information obtained as bearing (angle) measurements from both sensor arrays to give a better solution. Using data from two different sensor arrays reduces the problem of observability and the observer need not follow the S-maneuver to attain observability of the process. The performance of the fusion algorithm is comparable to that of theoretical Cramer–Rao lower bound and with that of the algorithm when bearing measurements from a single sensor array are considered. Different filters are used for analyzing both algorithms. Monte Carlo runs need to be done to evaluate the performance of algorithms more accurately. Also, the performance of the fusion algorithm is evaluated in terms of solution convergence time.
Utilization of ICT as a Digital Infrastructure Concerning Disaster Countermeasures in Japan
Authors: Yamamoto Kayoko
Published: September 2020

The present study aimed to describe the utilization of Information and Communication Technology (ICT) as a digital infrastructure concerning disaster countermeasures in Japan. Specifically, the study introduced development cases of the systems integrating social media and Geographic Information Systems (GIS), and presented the utilization potential as a digital infrastructure. Additionally, taking up Twitter as a familiar digital infrastructure, the study also presented its utilization potential based on the case of the heavy-rain disaster in Western Japan in 2018. As a result, due to the close relationship between the reality and virtual spaces, the issue is how to make the information circulating in the virtual space efficiently and effectively aid in the rescue and support activities in the reality space. The above systems are effective in order to solve such an issue, because these can efficiently consolidate the essential information on the digital map of Web-GIS. Additionally, it is necessary to set rules for the utilization of social media, and sift through information and share only the necessary information to the affected local governments and those involved in the rescue and support activities. Furthermore, various information communication methods including verbal calls in addition to ICT are necessary especially for people who are vulnerable to information.
Calculated vs. Ad Hoc Publics in the #Brexit Discourse on Twitter and the Role of Business Actors
Authors: Lynn Theo , Rosati Pierangelo , Nair Binesh
Published: September 2020

Mobilization theory posits that social media gives a voice to non-traditional actors in socio-political discourse. This study uses network analytics to understand the underlying structure of the Brexit discourse and whether the main sub-networks identify new publics and influencers in political participation, and specifically industry stakeholders. Content analytics and peak detection analysis are used to provide greater explanatory values to the organizing themes for these sub-networks. Our findings suggest that the Brexit discourse on Twitter can be largely explained by calculated publics organized around the two campaigns and political parties. Ad hoc communities were identified based on (i) the media, (ii) geo-location, and (iii) the US presidential election. Other than the media, significant sub-communities did not form around industry as whole or around individual sectors or leaders. Participation by business accounts in the Twitter discourse had limited impact.