bluuebunny/crossref_metadata_2025 huggingface dataset:
Increased lesion detectability in patients with locally advanced breast cancer—A pilot study using dynamic whole-body [18F]FDG PET/CT
Authors: Pedersen Mette Abildgaard , Dias André H. , Hjorthaug Karin , Gormsen Lars C. , Fledelius Joan , Johnsson Anna Lyhne , Borgquist Signe , Tramm Trine , Munk Ole Lajord , Vendelbo Mikkel Holm
Published: March 2024

Background

Accurate diagnosis of axillary lymph node (ALN) metastases is essential for prognosis and treatment planning in breast cancer. Evaluation of ALN is done by ultrasound, which is limited by inter-operator variability, and by sentinel lymph node biopsy and/or ALN dissection, none of which are without risks and/or long-term complications. It is known that conventional 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) positron emission tomography/computed tomography (PET/CT) has limited sensitivity for ALN metastases. However, a recently developed dynamic whole-body (D-WB) [18F]FDG PET/CT scanning protocol, allowing for imaging of tissue [18F]FDG metabolic rate (MRFDG), has been shown to have the potential to increase lesion detectability. The study purpose was to examine detectability of malignant lesions in D-WB [18F]FDG PET/CT compared to conventional [18F]FDG PET/CT.

Results

This study prospectively included ten women with locally advanced breast cancer who were referred for an [18F]FDG PET/CT as part of their diagnostic work-up. They all underwent D-WB [18F]FDG PET/CT, consisting of a 6 min single bed dynamic scan over the chest region started at the time of tracer injection, a 64 min dynamic WB PET scan consisting of 16 continuous bed motion passes, and finally a contrast-enhanced CT scan, with generation of MRFDG parametric images. Lesion visibility was assessed by tumor-to-background and contrast-to-noise ratios using volumes of interest isocontouring tumors with a set limit of 50% of SUVmax and background volumes placed in the vicinity of tumors. Lesion visibility was best in the MRFDG images, with target-to-background values 2.28 (95% CI: 2.04–2.54) times higher than target-to-background values in SUV images, and contrast-to-noise values 1.23 (95% CI: 1.12–1.35) times higher than contrast-to-noise values in SUV images. Furthermore, five imaging experts visually assessed the images and three additional suspicious lesions were found in the MRFDG images compared to SUV images; one suspicious ALN, one suspicious parasternal lymph node, and one suspicious lesion located in the pelvic bone.

Conclusions

D-WB [18F]FDG PET/CT with MRFDG images show potential for improved lesion detectability compared to conventional SUV images in locally advanced breast cancer. Further validation in larger cohorts is needed.

Clinical trial registration

The trial is registered in clinicaltrials.gov, NCT05110443, https://www.clinicaltrials.gov/study/NCT05110443?term=NCT05110443&rank=1.
CG-PBFT: an efficient PBFT algorithm based on credit grouping
Authors: Liu Juan , Deng Xiaohong , Li Wangchun , Li Kangting
Published: March 2024

Because of its excellent properties of fault tolerance, efficiency and availability, the practical Byzantine fault tolerance (PBFT) algorithm has become the mainstream consensus algorithm in blockchain. However, current PBFT algorithms have problems such as inadequate security of primary node selection, high communication overhead and network delay in the process of consensus. To address these problems, we design a novel efficient Byzantine fault tolerance algorithm based on credit grouping, called CG-PBFT. First, we propose a new credit evaluation model to obtain nodes’ credit values and introduce an optimized three-way quick sorting algorithm to divide nodes into the master-node group, the consensus-node group and the observation-node group, which have different privileges. The nodes in the observation-node group are restricted from participating in consensus, which reduces the communication overhead and improves consensus efficiency. Second, we propose an optimized selection method for the primary node based on a voting mechanism whereby the consensus-node group and observation-node group vote to produce the primary node, which reduces the probability of malicious nodes acting as the primary node and improves the security of primary node selection. Finally, the identity conversion mechanism between node groups is designed, and the actual behavior of nodes within different groups is given credit rewards or punishment, so as to keep an incentive for nodes to participate in appropriate system behavior and improve the working enthusiasm of nodes. The experimental simulation results show that compared with existing PBFT algorithms, the CG-PBFT algorithm improves the average throughput by 51.3% and reduces the average delay by 64.5%; it greatly improves the operating efficiency of the system and can be more suitable for application in the consortium blockchain scenarios.
Anesthesia decision analysis using a cloud-based big data platform
Authors: Zhang Shuiting , Li Hui , Jing Qiancheng , Shen Weiyun , Luo Wei , Dai Ruping
Published: March 2024

Big data technologies have proliferated since the dawn of the cloud-computing era. Traditional data storage, extraction, transformation, and analysis technologies have thus become unsuitable for the large volume, diversity, high processing speed, and low value density of big data in medical strategies, which require the development of novel big data application technologies. In this regard, we investigated the most recent big data platform breakthroughs in anesthesiology and designed an anesthesia decision model based on a cloud system for storing and analyzing massive amounts of data from anesthetic records. The presented Anesthesia Decision Analysis Platform performs distributed computing on medical records via several programming tools, and provides services such as keyword search, data filtering, and basic statistics to reduce inaccurate and subjective judgments by decision-makers. Importantly, it can potentially to improve anesthetic strategy and create individualized anesthesia decisions, lowering the likelihood of perioperative complications.
A genetic survey of patients with familial idiopathic intracranial hypertension residing in a Middle Eastern village: genetic association study
Authors: Berkowitz Eran , Falik Zaccai Tzipora C. , Irge Dana , Gur Inbar , Tiosano Beatrice , Kesler Anat
Published: March 2024

Background

The aim of this study was to determine whether genetic variants are associated with idiopathic intracranial hypertension (IIH) in a unique village where many of the IIH patients have familial ties, a homogenous population and a high prevalence of consanguinity. Several autosomal recessive disorders are common in this village and its population is considered at a high risk for genetic disorders.

Methods

The samples were genotyped by the Ilumina OmniExpress-24 Kit, and analyzed by the Eagle V2.4 and DASH software package to cluster haplotypes shared between our cohort. Subsequently, we searched for specific haplotypes that were significantly associated with the patient groups.

Results

Fourteen patients and 30 controls were included. Samples from 22 female participants (11 patients and 11 controls) were evaluated for haplotype clustering and genome-wide association studies (GWAS). A total of 710,000 single nucleotide polymorphisms (SNPs) were evaluated. Candidate areas positively associated with IIH included genes located on chromosomes 16, 8 (including the CA5A and BANP genes, p < 0.01), and negatively associated with genes located on chromosomes 1 and 6 (including PBX1, LMX1A, ESR1 genes, p < 0.01).

Conclusions

We discovered new loci possibly associated with IIH by employing a GWAS technique to estimate the associations with haplotypes instead of specific SNPs. This method can in all probability be used in cases where there is a limited amount of samples but strong familial connections. Several loci were identified that might be strong candidates for follow-up studies in other well-phenotypes cohorts.
Ecological patterns and processes of temporal turnover within lung infection microbiota
Authors: Gavillet Helen , Hatfield Lauren , Jones Andrew , Maitra Anirban , Horsley Alexander , Rivett Damian , van der Gast Christopher
Published: March 2024

Background

Chronic infection and consequent airway inflammation are the leading causes of morbidity and early mortality for people living with cystic fibrosis (CF). However, lower airway infections across a range of chronic respiratory diseases, including in CF, do not follow classical ‘one microbe, one disease’ concepts of infection pathogenesis. Instead, they are comprised of diverse and temporally dynamic lung infection microbiota. Consequently, temporal dynamics need to be considered when attempting to associate lung microbiota with changes in disease status. Set within an island biogeography framework, we aimed to determine the ecological patterns and processes of temporal turnover within the lung microbiota of 30 paediatric and adult CF patients prospectively sampled over a 3-year period. Moreover, we aimed to ascertain the contributions of constituent chronic and intermittent colonizers on turnover within the wider microbiota.

Results

The lung microbiota within individual patients was partitioned into constituent chronic and intermittent colonizing groups using the Leeds criteria and visualised with persistence-abundance relationships. This revealed bacteria chronically infecting a patient were both persistent and common through time, whereas intermittently infecting taxa were infrequent and rare; respectively representing the resident and transient portions of the wider microbiota. It also indicated that the extent of chronic colonization was far greater than could be appreciated with microbiological culture alone. Using species-time relationships to measure temporal turnover and Vellend’s rationalized ecological processes demonstrated turnover in the resident chronic infecting groups was conserved and underpinned principally by the deterministic process of homogenizing dispersal. Conversely, intermittent colonizing groups, representing newly arrived immigrants and transient species, drove turnover in the wider microbiota and were predominately underpinned by the stochastic process of drift. For adult patients, homogenizing dispersal and drift were found to be significantly associated with lung function. Where a greater frequency of homogenizing dispersal was observed with worsening lung function and conversely drift increased with better lung function.

Conclusions

Our work provides a novel ecological framework for understanding the temporal dynamics of polymicrobial infection in CF that has translational potential to guide and improve therapeutic targeting of lung microbiota in CF and across a range of chronic airway diseases.
Is convenience really king? Comparative evaluation of catastrophic costs due to tuberculosis in the public and private healthcare sectors of Viet Nam: a longitudinal patient cost study
Authors: Nguyen Hoa Binh , Vo Luan Nguyen Quang , Forse Rachel Jeanette , Wiemers Anja Maria Christine , Huynh Huy Ba , Dong Thuy Thi Thu , Phan Yen Thi Hoang , Creswell Jacob , Dang Thi Minh Ha , Nguyen Lan Huu , Shedrawy Jad , Lönnroth Knut , Nguyen Tuan Dinh , Dinh Luong Van , Annerstedt Kristi Sidney , Codlin Andrew James
Published: March 2024

Background

In Viet Nam, tuberculosis (TB) represents a devastating life-event with an exorbitant price tag, partly due to lost income from daily directly observed therapy in public sector care. Thus, persons with TB may seek care in the private sector for its flexibility, convenience, and privacy. Our study aimed to measure income changes, costs and catastrophic cost incurrence among TB-affected households in the public and private sector.

Methods

Between October 2020 and March 2022, we conducted 110 longitudinal patient cost interviews, among 50 patients privately treated for TB and 60 TB patients treated by the National TB Program (NTP) in Ha Noi, Hai Phong and Ho Chi Minh City, Viet Nam. Using a local adaptation of the WHO TB patient cost survey tool, participants were interviewed during the intensive phase, continuation phase and post-treatment. We compared income levels, direct and indirect treatment costs, catastrophic costs using Wilcoxon rank-sum and chi-squared tests and associated risk factors between the two cohorts using multivariate regression.

Results

The pre-treatment median monthly household income was significantly higher in the private sector versus NTP cohort (USD 868 vs USD 578; P = 0.010). However, private sector treatment was also significantly costlier (USD 2075 vs USD 1313; P = 0.005), driven by direct medical costs which were 4.6 times higher than costs reported by NTP participants (USD 754 vs USD 164; P < 0.001). This resulted in no significant difference in catastrophic costs between the two cohorts (Private: 55% vs NTP: 52%; P = 0.675). Factors associated with catastrophic cost included being a single-person household [adjusted odds ratio (aOR = 13.71; 95% confidence interval (CI): 1.36–138.14; P = 0.026], unemployment during treatment (aOR = 10.86; 95% CI: 2.64–44.60; P < 0.001) and experiencing TB-related stigma (aOR = 37.90; 95% CI: 1.72–831.73; P = 0.021).

Conclusions

Persons with TB in Viet Nam face similarly high risk of catastrophic costs whether treated in the public or private sector. Patient costs could be reduced through expanded insurance reimbursement to minimize direct medical costs in the private sector, use of remote monitoring and multi-week/month dosing strategies to avert economic costs in the public sector and greater access to social protection mechanism in general.
Development and validation of the perceived symptom manageability scale among people living with the human immunodeficiency virus
Authors: Xie Meilian , Wang Aiping , Zhang Zhiyun , Wang Kerong , Yu Yanping , Ma Xiaojing , Lin Zhaoxia , Yu Zhengli , Ke Jianxue
Published: March 2024

Background

“Perceived Symptom Manageability (PSM)” is essential in symptom management among people living with HIV. As a standardized assessment instrument was lacking, we developed a PSM scale for people living with human immunodeficiency virus (PSM-HIV).

Methods

Data analysis was performed using the sample from HIV-designated medical institutions (N = 540). Psychometric testing, namely reliability and validity, is assessed by unidimensionality, internal consistency, exploratory and confirmatory factor analysis, and structural equation modeling.

Results

The final version of the PSM- HIV scale contained 15 items. This scale was submitted to a principal components analysis with varimax rotation, and three factors were obtained, explained by a total variance of 63.10%. The three factors were named Cognitive-Behavioral, Affective Interaction, and Self-Attitude. The results show that the scale had high reliability, Cronbach’s α of the scale ranged from 0.71 to 0.92, and the Intraclass Correlation Coefficient was 0.88. The structural equation model supports a factor model with the acceptable fit (χ2/df (CMIN/DF) = 2.50, Root Mean square Residual (RMR) = 0.03, Goodness-of-Fit Index (GFI) = 0.93, Adjusted Goodness of Fit Index (AGFI) = 0.90, Normed Fit Index (NFI) = 0.93, Incremental Fit Index (IFI) = 0.96, Comparative Fit Index (CFI) = 0.96). The average variance extracted was 0.38 ∼ 0.59, and the composite reliability was 0.70 ∼ 0.91, indicating that the convergent validity of the scale is acceptable. Subjects with different stages of the disease reached significance(χ2 = 9.02; df = 2, P<0.05), meaning moderate Known-Groups Comparison Validation.

Conclusions

The PSM-HIV scale is a valid instrument that measures overall attitude and belief about controlling or coping with HIV-relevant symptoms.
“Examining the link between tooth agenesis and papillary thyroid cancer: is there a risk factor?” Observational study
Authors: Matošić Željana , Šimunović Luka , Jukić Tomislav , Granić Roko , Meštrović Senka
Published: March 2024

Background

Mutations in one or multiple genes can lead to hypodontia and its characteristic features. Numerous studies have shown a strong genetic influence on the occurrence of hypodontia, and identified several genes, including AXIN2, EDA, FGF3, FGFR2, FGFR10, WNT10A, MSX1, and PAX9, that are directly associated with dental agenesis and carcinogenesis. The objective of this study was to investigate the occurrence and pattern of tooth agenesis, microdontia, and palatally displaced canine (PDC) in women diagnosed with papillary thyroid cancer (PTC), compared to a control group of women without any malignancy or thyroid disease.

Materials and methods

This case–control study was carried at the Department of Orthodontics, School of Dental Medicine University of Zagreb, and Department of Oncology and Nuclear Medicine Sestre Milosrdnice University Hospital Centre. The study involved a clinical examination and evaluation of dental status, panoramic X-ray analysis, and assessment of medical and family history of 116 female patients aged 20–40 with PTC, as well as 424 females in the control group who were of similar age.

Results

The prevalence of hypodontia, microdontia, and PDC was statistically higher in women with PTC than in the control group. The prevalence rate of hypodontia was 11.3% in the experimental group and 3.5% in the control group. The experimental group showed a higher occurrence of missing upper lateral incisors, lower left central incisors, and all the third molars (except the upper left) compared to the control group. Women with PTC showed the prevalence of PDC significantly higher than the control group (3.5%, 0.7%, p = 0.002). The probability of hypodontia as a clinical finding increases 2.6 times, and microdontia occurs 7.7 times more frequently in women with PTC.

Conclusion

Our study suggests a possible link between odontogenesis and PTC. The absence of permanent teeth may increase the likelihood of PTC in women. Leveraging the age-7 orthopantomogram to identify women at high risk for PTC within a critical early detection window could significantly improve oral health outcomes and PTC prognosis through proactive interventions.
Regional event-based flood quantile estimation method for large climate projection ensembles
Authors: Chen Jiachao , Sayama Takahiro , Yamada Masafumi , Sugawara Yoshito
Published: March 2024

Emerging large ensemble climate datasets produced by multiple general circulation models and their downscaling products challenge the limits of hydrodynamic models because of the immense data size. To overcome this new challenge and estimate the discharge quantiles corresponding to different return periods at all river sections in an entire region, this study proposes an event-based regional approach that uses a nationwide distributed rainfall–runoff model as well as large climate projection ensembles. This approach addresses the high computational burden associated with continuous simulations and solves the problem of conventional event-based simulations serving only a single outlet of a basin. For our analysis, we extracted 372 annual maximum 48 h rainfall events that cover the entirety of Shikoku Island and its eight major river basins. Peak discharges were estimated using a 150 m resolution rainfall–runoff–inundation model. These discharges were then screened using either the peak-over-threshold (POT) method or block maxima (BM) method, and frequency curves were subsequently constructed and evaluated. The primary reason for the necessity of POT or BM was to avoid interference from extraneous low discharges. The POT-based frequency curves showed good accuracy when using peak discharges in the range of the top 10–50%, and the results remain stable within this threshold range. The BM method, employing block sizes of 2–5 years, can generate relatively accurate frequency curves, but the choice of block size introduces significant variations in results among certain basins. Generally, the accuracy of results based on the POT method surpasses that of the BM method. Considering the accuracy, computational cost, and result stability, the POT method is preferred. The error introduced by the regional approach was acceptable with more than half of the relative root-mean-square errors remaining within 10% and basically all of the results are within 20%. The results of the regional approach exhibited good accuracy across climate scenarios and provided consistent information regarding future flood quantiles. This study serves as the foundation for high-resolution future flood risk assessment.
Optimal portfolio selection with volatility information for a high frequency rebalancing algorithm
Authors: Bağcı Mahmut , Soylu Pınar Kaya
Published: March 2024

We propose a high-frequency rebalancing algorithm (HFRA) and compare its performance with periodic rebalancing (PR) and threshold rebalancing (TR) strategies. PR refers to the process of adjusting the relative weight of assets within portfolios at regular time intervals, whereas TR is a process of setting allocation limits for portfolios and rebalancing when portfolios exceed a specific percentage of deviation from the target allocation. The HFRA is constructed as an integration of pairs trading and a threshold-based rebalancing strategy, and the profitability of the HFRA is examined to determine the optimal portfolio size. The HFRA is applied to a dataset of real price series from cryptocurrency exchange markets across various trends and volatility regimes. Using cointegrated price data, it is shown that increasing the number of assets in a portfolio supports the profitability of the HFRA in an up-trend and reduces the potential loss of the HFRA in a down-trend in a high-volatility environment. For low-volatility regimes, although increasing portfolio size marginally enhances the HFRA’s profitability, the profits of portfolios of varied sizes do not significantly differ. It is demonstrated that when volatility is relatively high and the trend is upward, the HFRA can yield a substantial return via portfolios of large sizes. Moreover, the profitability of the HFRA is compared with that of the PR and TR strategies for long-term application. The HFRA is more profitable than the PR and TR strategies. This achievement of the HFRA is also validated statistically using the Fisher–Pitman permutation test.
Generative AI and the future of higher education: a threat to academic integrity or reformation? Evidence from multicultural perspectives
Authors: Yusuf Abdullahi , Pervin Nasrin , Román-González Marcos
Published: March 2024

In recent years, higher education (HE) globally has witnessed extensive adoption of technology, particularly in teaching and research. The emergence of generative Artificial Intelligence (GenAI) further accelerates this trend. However, the increasing sophistication of GenAI tools has raised concerns about their potential to automate teaching and research processes. Despite widespread research on GenAI in various fields, there is a lack of multicultural perspectives on its impact and concerns in HE. This study addresses this gap by examining the usage, benefits, and concerns of GenAI in higher education from a multicultural standpoint. We employed an online survey that collected responses from 1217 participants across 76 countries, encompassing a broad range of gender categories, academic disciplines, geographical locations, and cultural orientations. Our findings revealed a high level of awareness and familiarity with GenAI tools among respondents. A significant portion had prior experience and expressed the intention to continue using these tools, primarily for information retrieval and text paraphrasing. The study emphasizes the importance of GenAI integration in higher education, highlighting both its potential benefits and concerns. Notably, there is a strong correlation between cultural dimensions and respondents’ views on the benefits and concerns related to GenAI, including its potential as academic dishonesty and the need for ethical guidelines. We, therefore, argued that responsible use of GenAI tools can enhance learning processes, but addressing concerns may require robust policies that are responsive to cultural expectations. We discussed the findings and offered recommendations for researchers, educators, and policymakers, aiming to promote the ethical and effective integration of GenAI tools in higher education.
Captopril pretreatment augments diabetogenic response to streptozotocin administration: experimental in vivo rat model
Authors: Ateyya Hayam , ShamsEldeen Asmaa Mohammed , Hosny Sara Adel , Kamar Samaa Samir , Rashed Laila Ahmed , Mostafa Abeer , Harb Inas
Published: March 2024

Background

Streptozotocin (STZ) is a glucose analogue commonly used for inducing diabetes in experimental animals. This study is intended to investigate the ability of captopril (Cap) pretreatment to augment STZ-induced diabetogenic effect in an experimental rat model. If this hypothesis were proven, Cap administration to rats could reduce the dosage of STZ by augmenting its effect and resulting in a subsequent reduction in STZ cost. Forty-two adult male Wistar rats were randomly divided into seven groups: a control group that fed a normal diet, whereas the other six experimental groups were fed a high-fat diet (HFD). The six groups were then divided into STZ-30, STZ-30-Cap, STZ-40, STZ-40-Cap, STZ-50, and STZ-50-Cap. All Cap-received groups were supplemented with 50 mg/kg Cap orally one hour just before intraperitoneal (I.P.) injection of STZ. 30-STZ, 40-STZ, and 50-STZ-treated groups were injected once with STZ I.P. at doses of 30, 40, and 50 mg/kg, respectively. An intraperitoneal glucose tolerance test (IPGTT) was done. Pancreatic tissue was obtained to measure Tumor necrosis factor alpha (TNF-α), interleukin one beta (IL-1β), and nitric oxide (NO) by enzyme-linked immunosorbent assay (ELISA) and glucose transporter 2 (GLUT2) gene expression by reverse transcription polymerase chain reaction (RT-PCR). Pancreatic sections were examined by hematoxylin and eosin (H&E) stain, and immunohistochemical staining by anti-insulin and anti-TNF-α antibodies.

Results

Results indicated that administration of Cap before STZ in different doses significantly augmented the hyperglycemic state that was evident by intraperitoneal glucose tolerance test, and markedly increased pancreatic pro-inflammatory markers. Histological analysis of islets of Langerhans indicated degeneration with extensive vacuolations associated with a significant decrease in mean area % of insulin immunoreactivity and an increase in optical density of TNF-α immunoreactivity.

Conclusion

These findings pointed to the ability of captopril pretreatment to augment the hyperglycemic state and the diabetogenic response that was induced secondary to STZ injection in an experimental rat model.
Investigating the causes of permeability anisotropy in heterogeneous conglomeratic sandstone using multiscale digital rock
Authors: Chi Peng , Sun Jianmeng , Yan Weichao , Luo Xin , Feng Ping
Published: March 2024

Heterogeneous conglomeratic sandstone exhibits anisotropic physical properties, rendering a comprehensive analysis of its physical processes challenging with experimental measurements. Digital rock technology provides a visual and intuitive analysis of the microphysical processes in rocks, thereby aiding in scientific inquiry. Nevertheless, the multiscale characteristics of conglomeratic sandstone cannot be fully captured by a single-scale digital rock, thus limiting its ability to characterize the pore structure. Our work introduces a proposed workflow that uses multiscale digital rock fusion to investigate permeability anisotropy in heterogeneous rock. We use a cycle-consistent generative adversarial network to fuse computed tomography scan data of different resolutions, creating a large-scale and high-precision digital rock that comprehensively represents the conglomeratic sandstone pore structure. Subsequently, the digital rock is partitioned into multiple blocks, and the permeability of each block is simulated using a pore network. Finally, the total permeability of the sample is calculated by conducting an upscaling numerical simulation using the Darcy-Stokes equation. This process facilitates the analysis of the pore structure in conglomeratic sandstone and provides a step-by-step solution for permeability. From a multiscale perspective, this approach reveals that the anisotropy of permeability in conglomeratic sandstone stems from the layered distribution of grain sizes and differences in grain arrangement across different directions.
Interdisciplinary public engagement: untapped potential?
Authors: Stott Janet , Vicente Catarina C.
Published: March 2024

Public engagement projects developed by university-based academics tend to focus on specific academic topics. Yet, the problems and topics that our audiences want to engage with are broad, challenging, and can't be explained or solved by a single academic subject or expertise. In this article, we capitalise on our experience working with academics at the University of Oxford, and a workshop for public engagement professionals that we co-organised with the National Coordinating Centre for Public Engagement, to advocate for a novel approach: interdisciplinary public engagement (public engagement projects that bring together academics from several academic disciplines). We consider the potential benefits and the challenges of this approach and provide examples of how it is starting to be explored.
P2X7 regulates ependymo-radial glial cell proliferation in adult Danio rerio following spinal cord injury
Authors: Stefanova Eva E. , Dychiao Julian Vincent T. , Chinn Mavis C. , Borhani Matin , Scott Angela L.
Published: March 2024

In contrast to mammals, zebrafish undergo successful neural regeneration following spinal cord injury. Spinal cord ependymo-radial glia (ERG) undergo injury-induced proliferation and neuronal differentiation to replace damaged cells and restore motor function. However, the molecular cues driving these processes remain elusive. Here, we demonstrate that the evolutionarily conserved P2X7 receptors are widely distributed on neurons and ERG within the zebrafish spinal cord. At the protein level, the P2X7 receptor expressed in zebrafish is a truncated splice variant of the full-length variant found in mammals. The protein expression of this 50 kDa isoform was significantly downregulated at 7 days post-injury (dpi) but returned to basal levels at 14 dpi when compared to naïve controls. Pharmacological activation of P2X7 following SCI resulted in a greater number of proliferating cells around the central canal by 7 dpi but did not affect neuronal differentiation at 14 dpi. Our findings suggest that unlike in mammals, P2X7 signaling may not play a maladaptive role following SCI in adult zebrafish and may also work to curb the proliferative response of ERG following injury.
Seed preference is only weakly linked to seed-type-specific feeding performance in a songbird
Authors: Andries Tim , Müller Wendt , Van Wassenbergh Sam
Published: March 2024

The dehusking of seeds by granivorous songbirds is a complex process that requires fast, coordinated and sensory-feedback-controlled movements of beak and tongue. Hence, efficient seed handling requires a high degree of sensorimotoric skill and behavioural flexibility, since seeds vary considerably in size, shape and husk structure. To deal with this variability, individuals might specialise on specific seed types, which could result in greater seed handling efficiency of the preferred seed type, but lower efficiency for other seed types. To test this, we assessed seed preferences of canaries (Serinus canaria) through food choice experiments and related these to data of feeding performance, seed-handling skills and beak kinematics during feeding on small, spindle-shaped canary seeds and larger, spheroid-shaped hemp seeds. We found great variety in seed preferences among individuals: some had no clear preference, while others almost exclusively fed on hemp seeds, or even prioritized novel seed types (millet seed). Surprisingly, we only observed few and weak effects of seed preference on feeding efficiency. This suggests either that the ability to handle seeds efficiently can be readily applied across various seed types, or alternatively, that achieving high levels of seed-specific handling skills does not require extensive practice.
Evaluation of the relationship between oral glucose tolerance test, blood sugar levels and cholesterol levels in prediabetic individuals
Authors: Oduncu Esra Aksoy , Tural Egemen , Dayan Akin
Published: March 2024

Objective: This study aimed to investigate whether there is a difference in lipid levels between prediabetic individuals with one-hour post-Oral Glucose Tolerance Test (OGTT) plasma glucose (PG) values > 155 mg/dl and those with one-hour PG values ≤ 155 mg/dl.

Methods: This retrospective cross sectional study was initiated on August 2020 and concluded on June 2021, and conducted with 229 prediabetic patients who presented to the Diabetes Clinic of the Research Hospital. A correlation analysis was performed to investigate the relationship between OGTT values and serum lipid levels. Additionally, the patients were divided into two groups based on the one-hour post-OGTT PG value of 155 mg/dl, and the presence of any difference in serum lipid levels between the two groups was examined using the Mann-Whitney U test. The SPSS 20 software was used for statistical analysis, and a statistical significance level of P < 0.05 was considered.

Results: Out of the 229 prediabetic patients included in the study, 172 were female. The number of patients with one-hour post-OGTT PG ≤ 155 mg/dl was 86, while those with values > 155 mg/dl were 143. A statistically significant difference was found between the group with one-hour post-OGTT PG > 155 mg/dl and the group with ≤ 155 mg/dl in terms of high-density lipoprotein (HDL-C) and triglyceride (TG) levels. There was a statistically significant negative correlation between one-hour PG and HDL-C.

Conclusion: The evaluation of HDL-C and TG levels is important in prediabetic patients with a one-hour OGTT PG level greater than 155 mg/dL. 

doi: https://doi.org/10.12669/pjms.40.5.8292

How to cite this: Oduncu EA, Tural E, Dayan A. Evaluation of the relationship between oral glucose tolerance test, blood sugar levels and cholesterol levels in prediabetic individuals. Pak J Med Sci. 2024;40(5):---------. doi: https://doi.org/10.12669/pjms.40.5.8292

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Outcome of the incarcerated abdominal wall hernias managed by open and laparoscopic approaches
Authors: AlWadaani Hamed A , MEMON ABDUL QADEER
Published: March 2024

Background & Objective: Laparoscopic surgery is generally considered as better than open surgery in terms of less complications, minimal hospital stays and quick healing of the wounds. Our objective was to compare the immediate and early outcome of the different incarcerated hernias of anterior abdominal wall operated on as emergency cases by open and laparoscopic approach.

Methods: This is a retrospective comparative study which was conducted at two hospitals of AlAhsa city of the Eastern region of the Kingdom of Saudi Arabia from July, 2017 to June, 2022. The data were retrieved from the medical records of the hospitals. All male and female patients having different types of incarcerated hernias of anterior abdominal wall presenting to the emergency room were included in the study. The patients were divided in two groups; those who were operated on by open approach (Group-I) and those who were operated on by laparoscopic approach (Group-II).

Results: Out of total 70 male and female patients, 42 were in Group-I and 28 in Group-II. The variety of the incarcerated hernias in both groups overall was para-umbilical 26(37.14%), incisional 18(25.71%), inguinal (right & left) 17(24.28%) and epigastric 9(12.86%). The mean operative time taken by Group I and II was 126.07 (±9.728) and 98.57 (±10.079) minutes respectively with a difference of 27.50 minutes (p=0.807). The mean hospital stay of the patients in Group I and II was 1.36(±0.719) and 1.57(±0.997) days respectively (p=0.482). The post-operative complications rate in Group-I was 6(14.28%) and in Group-II, 6(21.43%) (p=0.658). Overall, 12(17.14%) patients developed the complications in both groups. When the number of the complications is compared, it shows that there was no significant difference between the two groups (p=0.583).

Conclusion: Laparoscopic approach is not superior to the open approach in the terms of the immediate and early outcome/complications of the incarcerated hernias of the anterior abdominal wall operated as emergency cases in this study.

doi: https://doi.org/10.12669/pjms.40.5.8899

How to cite this: AlWadaani HA, Memon AQ. Outcome of the incarcerated abdominal wall hernias managed by open and laparoscopic approaches. Pak J Med Sci. 2024;40(5):---------. doi: https://doi.org/10.12669/pjms.40.5.8899

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Critical thinking about treatment effects in Eastern Africa: development and Rasch analysis of an assessment tool
Authors: Dahlgren Astrid , Semakula Daniel , Chesire Faith , Mugisha Michael , Nakyejwe Esther , Nsangi Allen , Nyirazinyoye Laetitia , Ochieng Marlyn A. , Oxman Andrew David , Ssenyonga Ronald , Simbi Clarisse Marie Claudine
Published: March 2024

Background:  Every day we are faced with different treatment claims, in the news, in social media, and by our family and friends. Some of these claims are true, but many are unsubstantiated. Without being supported by reliable evidence such guidance can lead to waste and harmful health choices. The Informed Health Choices (IHC) Network facilitates development of interventions for teaching children and adults the ability to assess treatment claims (informedhealthchoices.org). Our objective was to develop and evaluate a new assessment tool developed from the item bank for use in an upcoming trial of lower secondary school resources in Uganda, Kenya, and Rwanda.

Methods: A cross-sectional study evaluating a questionnaire including two item-sets was used. The first evaluated ability using multiple-choice questions (scored dichotomously) and the other evaluated intended behaviour and self-efficacy (measured using Likert scales). This study was conducted in Uganda, Kenya, and Rwanda in 2021. We recruited children (over 12 years old) and adults through schools and our networks. We entered 1,671 responses into our analysis. Summary and individual fit to the Rasch model (including Cronbach’s Alpha) were assessed using the RUMM2030 software.

Results: Both item-sets were found to have good fit to the Rasch model and were acceptable to our target audience. The reliability was good (Cronbach’s alpha >0.7). Observations of the individual item and person fit provided us with guidance on how we could improve the design, scoring, and administration of the two item-sets. There was no local dependency in either of the item-sets, and both item-sets were found to have acceptable unidimensionality.

Conclusion: To our knowledge, this is the first instrument validated for measuring ability to assess treatment claims in Uganda, Kenya and Rwanda. Overall, the two item-sets were found to have satisfactory measurement properties.
Effects of a low, medium, and high-intensity aquatic physiotherapy protocol on functional and biochemical parameters in individuals with knee osteoarthritis: protocol for a crossover randomized controlled trial
Authors: Augusto Teixeira Cesar , Haas Lisete , Frata Bruna , Fiori Bortoli Andressa , Scalco Acco Fernanda , de Castro Gustavo , Cechetti Fernanda
Published: March 2024

Background Knee osteoarthritis affects the performance of daily activities, independence, and quality of life. The etiopathogenesis of this condition considers the mechanisms of activation of metalloproteinase and reactive oxygen species production pathways. Metalloproteinases-3 (MMP-3) and Glutathione Peroxidase (GPx) may be responsible for cartilage destruction. Aquatic physiotherapy promotes a positive impact on the clinical picture of osteoarthritis, and this study presents an intervention protocol that aims to evaluate the effects of a single session of different aquatic physiotherapy modalities on the biochemical and functional behavior of patients with knee osteoarthritis. Methods This will be a crossover randomized controlled trial in which 15 individuals will be submitted to three aquatic physiotherapy modalities with a minimum 15-day wash-out period in patients over 50 years old and diagnosed with OA in at least one knee, presence of pain and at least one functional dysfunction for at least 6 months, absence of physical limitation that prevents the exercise protocol from being performed, Kellgren and Lawrence ranking between I and IV, walk independently and without auxiliary device. Variations in the concentrations of MMP-3 and GPx in peripheral blood, pain, edema, and flexibility resulting from the three aquatic physiotherapeutic interventions will be evaluated both pre- and immediate post-intervention. The reference group will be submitted to the same aquatic physiotherapy protocols, however, only the biochemical parameters and the self-perception questionnaires will be evaluated. Registration ClinicalTrials.gov (NCT05610696, 18/01/2023).
Outcome-Driven Dynamic Refugee Assignment with Allocation Balancing
Authors: Bansak Kirk , Paulson Elisabeth
Published: March 2024

Dynamic Assignment Algorithms to Boost Refugee Outcomes

Amid record-breaking forced displacement in recent years, researchers and policymakers alike have become increasingly interested in the idea of algorithmically matching refugees to geographic localities in order to optimize their employment or other integration outcomes. In “Outcome-Driven Dynamic Refugee Assignment with Allocation Balancing,” Bansak and Paulson propose new dynamic assignment algorithms for this context designed to maximize a given outcome while meeting the operational needs of refugee resettlement and asylum agencies. Using resettlement data from both the United States and Switzerland, they demonstrate how one algorithm (currently being piloted in Switzerland) can achieve near-optimal results compared with a hindsight-optimal matching. They also show that, because of nonstationarities in the arrival process, outcome maximization (even when subject to location capacity constraints) can result in an imbalanced allocation to localities over time, putting periodic strains on limited local resettlement resources. They account for this problem in a second algorithm that achieves near-perfect balance over time with only a small loss in average outcomes compared with the first algorithm.
O divórcio das teorias do aprendizado e a escola: reflexões gerais
Authors: Monteiro Leonardo Henrique Brandão
Published: March 2024

Este artigo expõe uma reflexão acerca de uma separação entre as teorias do aprendizado e a instituição escolar. O caráter do trabalho é o de uma análise teórica realizada através de uma revisão brevemente histórica e outra contextual de teorias contemporâneas do aprendizado, de modo a refletir sobre os diversos meandros presentes nesta produção, através de sua importância ao discutir processos de aprendizagem que ocorrem fora do ambiente escolar, ou, então, ao debater os modos como teorias do aprendizado que focam no “aprender a aprender”, cada vez mais frequentes no Brasil, apresentam certos perigos, quando não lidas criticamente. Por fim, apresentam-se alguns caminhos de pesquisa que podem ser desenvolvidos a partir do debatido neste paper.
Understanding Problem-Based Learning and its Application in Learning Mathematics Concepts Among Pre-Service Teachers
Authors: Boye Eric S. , Agyei Douglas D.
Published: March 2024

Learning to teach mathematics has become crucial since its application in real life cannot go unmentioned. The desire of mathematics education researchers to make mathematics concepts easier for pre-service teachers to easily understand has attracted attention. This has become indispensable since after college, pre-service teachers are deployed from K-12 to assist learners in understanding mathematics concepts. The study aimed to ascertain how improvement in the learning of mathematics concepts using the Problem-based learning (PBL) approach could be understood and/or explained among pre-service teachers. This was viewed in two folds: how improvement in learning outcomes using the PBL approach could be explained; and how pre-service teachers’ disposition about the PBL could be explained/understood. Exploratory case study design involving qualitative and quantitative data was concurrently gathered and used. This involved the use of data collection instruments such as focus group discussion, pre-post-test scores, PBL observation protocol, and PBL disposition questionnaire. The study showed that the PBL method improved the learning of mathematics concepts among pre-service teachers. Pre-service teachers also showed a positive disposition (interest, belief, and attitude) toward the PBL intervention. The authors advocated for the conduct of a longitudinal study to understand the direction of change over time.
A Sentence Medial Clause Typing Intoneme in Mandarin A-NOT-A Questions
Authors: Wang Chi
Published: March 2024

A sentence medial intoneme is argued to be responsible for typing Mandarin A-NOT-A questions. In narrow syntax, it is merged higher than deontic modals and lower than Tense; after spell out, it is realized phonetically as a high low intonation starting from the left edge of the A-NOT-A predicate. Th is proposal is supported by a sentence medial segmental particle in Chinese dialects, which further corroborates the Intonation Particle Hypothesis by showing that typing particles in non sentence final positions are also variants of intonations. Besides, the Mandarin nu clear stress rule derives two prosodic features of A-NOT-A questions : No intonational breaks within the A-NOT-A predicate and destressing of the negation word. The two features follow from the requirement that only one intonation is allowed in the nuclear stress domain and instantiate interaction among different stresses
Temperature-compensated high strain measurement based on a Fabry–Perot interferometer with the virtual-assisted Vernier effect
Authors: Zhang Tongqun , Tian Xiaozhong , Xie Kang , Wang Minghong , Wu Jintao , Ma Dianguo , Sun Yunqun , Wang Xueqin , Wang Junbao , Zheng Yanbin
Published: March 2024

A highly sensitive optical fiber Fabry–Perot interferometer (FPI) for strain measurement with temperature compensation is proposed. Instead of using another actual reference interferometer, a virtual FPI is constructed to superpose with the sensing FPI to form the Vernier effect. The fundamental and the first-order harmonic Vernier effect are generated to increase the sensitivity by adjusting the parameter of the virtual FPI. In order to separate the strain from the environment temperature, an FBG is cascaded to distinguish the applied temperature. Experimental results demonstrate that, with the help of the fundamental Vernier effect, the sensitivity and temperature of the FPI increases from 1.05 pm/°C to 10.63 pm/°C in the temperature range of 40-120°C, and the sensitivity of strain increases from 2.635 pm/µε to 33.11 pm/µε in the strain range of 0-400 µε. In order to access the tracking points more easily and further enhance the sensitivities, the first-order harmonic Vernier effect is generated by modifying the virtual FPI. Results show that the temperature and strain sensitivities are 21.25 pm/°C and 62.25 pm/µε, respectively. In addition, with the help of the FBG, the strain can be separated from the temperature by solving the cross-sensitivity matrix.