bluuebunny/crossref_metadata_2025 huggingface dataset:
Association Between Prior Calcium Channel Blocker Use and Mortality in Septic Patients: A Meta-Analysis of Cohort Studies
Authors: Ding Xianfei , Cui Yuqing , Liang Huoyan , Wang Dong , Li Lifeng , Kan Quancheng , Wang Lexin , Sun Tongwen
Published: May 2021

Background: The aim of this study was to comprehensively review the literature and synthesize the evidence concerning the relationship between prior calcium channel blocker (CCB) use and mortality in patients with sepsis.

Methods: The Medical Literature Analysis and Retrieval System Online (MEDLINE), Excerpta Medica database (EMBASE), Cochrane CENTRAL, and Web of Science databases were searched from their inception to April 9, 2020. Cohort studies related to prior calcium channel blocker use in patients with sepsis were analyzed. Pairs of reviewers independently screened the studies, extracted the data, and assessed the risk of bias. The primary outcome of 90-days mortality or secondary outcome of short-term mortality, including 30-days, Intensive Care Unit (ICU), and in-hospital mortality, were analyzed. Heterogeneity among studies was assessed using the I2 statistic and was considered moderate if I2 was 50–75% and high if I2 was ≥75%. Random-effects models were used to calculate the pooled odds ratios (ORs) and 95% confidence intervals (CIs). The quality of the studies was evaluated with the Newcastle-Ottawa Scale (NOS). Sensitivity analyses were performed to examine the robustness of the results.

Results: In total, 639 potentially relevant studies were identified, and the full texts of 25 articles were reviewed. Ultimately, five cohort studies involving 280,982 patients were confirmed to have a low risk of bias and were included. Prior CCB use was associated with a significantly lower 90-days mortality in sepsis patients [OR, 0.90 (0.85–0.95); I2 = 31.9%]. Moreover, prior CCB use was associated with a significantly reduced short-term mortality rate in septic shock patients [OR, 0.61 (0.38–0.97); I2 = 62.4%] but not in sepsis patients [OR, 0.83 (0.66–1.04); I2 = 95.4%].

Conclusion: This meta-analysis suggests that prior CCB use is significantly associated with improved 90-days mortality in sepsis patients and short-term mortality in septic shock patients. This study provides preliminary evidence of an association between prior CCB use and mortality in sepsis patients.
Real-world antibiotic use in treating acute exacerbations of chronic obstructive pulmonary disease (AECOPD) in China: Evidence from the ACURE study
Authors: Ma Yiming , Huang Ke , Liang Chen , Mao Xihua , Zhang Yaowen , Zhan Zijie , Yang Ting , Chen Yan
Published: May 2021

Background: The evidence for real-world antibiotic use in treating acute exacerbations of chronic obstructive pulmonary disease (AECOPD) is insufficient. This study aimed to investigate real-world antibiotic use in the management of AECOPD in China.

Methods: All hospitalized AECOPD patients from the acute exacerbation of chronic obstructive pulmonary disease inpatient registry (ACURE) study conducted at 163 sites between January 2018 and December 2019 were screened according to the eligible criteria. The eligible study population was divided into secondary and tertiary hospital groups. Patients’ baseline characteristics, antibiotic use, and bacterial pathogen characteristics were retrieved and analyzed using SPSS 23.0.

Results: A total of 1663 patients were included in the study, including 194 patients from secondary hospitals and 1469 patients from tertiary hospitals. Among the 1663 AECOPD patients enrolled, 1434 (86.2%) received antibiotic treatment, comprising approximately 85.6% and 86.3% of patients in the secondary and tertiary hospital groups, respectively. The median antibiotic therapy duration was 9.0 (interquartile range [IQR]: 7.0 - 11.0)°days. Regarding the routes of antibiotic use, 1400 (97.6%) patients received intravenous antibiotics, 18 (1.3%) patients received oral antibiotics, 15 (1.0%) patients received both intravenous and oral antibiotics, and one (0.1%) patient received both oral and nebulized antibiotic treatment. In addition, cephalosporin, penicillin, and quinolone were the most commonly prescribed antibiotics (43.6%, 37.0%, and 34.2%, respectively). In total, 990 (56.5%) patients underwent pathogen examinations; the proportion of patients receiving pathogen examinations in the second hospital group was significantly lower than that in the tertiary hospital group (46.4% vs 61.3%, p < 0.001).

Conclusion: This study demonstrates that an antibiotic overuse may exist in the treatment of AECOPD in China. Measures should be taken to prevent the overuse of antibiotics and potential antimicrobial resistance (AMR) in Chinese AECOPD patients.
Blockade of β-Adrenergic Receptors by Propranolol Disrupts Reconsolidation of Drug Memory and Attenuates Heroin Seeking
Authors: Chen Liangpei , Huang Shihao , Yang Chang , Wu Feilong , Zheng Qiuyao , Yan He , Yan Jie , Luo Yixiao , Galaj Ewa
Published: May 2021

Persistent traces of drug reward memories contribute to intense craving and often trigger relapse. A number of pharmacological interventions on drug-associated memories have shown significant benefits in relapse prevention at a preclinical level but their translational potential is limited due to deleterious side effects. Propranolol, a non-specific β-adrenergic receptors antagonist, is known for its ability to erase maladaptive memories associated with nicotine or cocaine in rodents and humans. However, little is known about its effect on reconsolidation of heroin memory and heroin seeking. In the present study, rats with a history of intravenous heroin self-administration received the propranolol treatment (10 mg/kg; i.p.) at different time windows with or without CS (conditioned stimulus) exposure. Our results showed that propranolol, when administered immediately after CS exposure but not 6 h later, can significantly attenuate cue-induced and drug-primed reinstatement of heroin seeking, suggesting that propranolol has the ability to disrupt heroin memory and reduce relapse. The propranolol treatment without retrieval of drug memory had no effect on subsequent reinstatement of heroin seeking, suggesting that its interfering effects are retrieval-dependent. Importantly, the effects of propranolol were long lasting as rats showed diminished drug seeking even 28 days after the treatment. Altogether, our study suggests that propranolol can interfere with reconsolidation of heroin memory and reduce subsequent drug seeking, making it an attractive therapeutic candidate for the treatment of opioid addiction and relapse prevention.
Efficient Genetic Transformation and Regeneration of a Farmer-Preferred Cassava Cultivar From Ghana
Authors: Elegba Wilfred , McCallum Emily , Gruissem Wilhelm , Vanderschuren Hervé
Published: May 2021

Cassava is an important staple crop that provides food and income for about 700 million Africans. Cassava productivity in Africa is limited by viral diseases, mainly cassava mosaic disease (CMD) and cassava brown streak disease (CBSD). Genetic barriers such as high heterozygosity, allopolyploidy, poor seed set, and irregular flowering constrain the development of virus-resistant cassava varieties via conventional breeding. Genetic transformation represents a valuable tool to circumvent several challenges associated with the development of virus resistance and other valuable agronomic traits in cassava. The implementation of genetic transformation in many local African cultivars is limited either by the difficulty to produce friable embryogenic callus (FEC), low transformation, and/or regeneration efficiencies. Here, we report the successful induction of organized embryogenic structures (OES) in 11 farmer-preferred cultivars locally grown in Ghana. The production of high quality FEC from one local cultivar, ADI 001, facilitated its genetic transformation with high shoot regeneration and selection efficiency, comparable to the model cassava cultivar 60444. We show that using flow cytometry for analysis of nuclear ploidy in FEC tissues prior to genetic transformation ensures the selection of genetically uniform FEC tissue for transformation. The high percentage of single insertion events in transgenic lines indicates the suitability of the ADI 001 cultivar for the introduction of virus resistance and other useful agronomic traits into the farmer-preferred cassava germplasm in Ghana and Africa.
Microgametophyte Development in Cannabis sativa L. and First Androgenesis Induction Through Microspore Embryogenesis
Authors: Galán-Ávila Alberto , García-Fortea Edgar , Prohens Jaime , Herraiz Francisco Javier
Published: May 2021

Development of double haploids is an elusive current breeding objective in Cannabis sativa L. We have studied the whole process of anther and pollen grain formation during meiosis, microsporogenesis, and microgametogenesis and correlated the different microgametophyte developmental stages with bud length in plants from varieties USO31 and Finola. We also studied microspore and pollen amyloplast content and studied the effect of a cold pretreatment to excised buds prior to microspore in vitro culture. Up to 476,903 microspores and pollen grains per male flower, with in vivo microspore viability rates from 53.71 to 70.88% were found. A high uniformity in the developmental stage of microspores and pollen grains contained in anthers was observed, and this allowed the identification of bud length intervals containing mostly vacuolate microspores and young bi-cellular pollen grains. The starch presence in C. sativa microspores and pollen grains follows a similar pattern to that observed in species recalcitrant to androgenesis. Although at a low frequency, cold-shock pretreatment applied on buds can deviate the naturally occurring gametophytic pathway toward an embryogenic development. This represents the first report concerning androgenesis induction in C. sativa, which lays the foundations for double haploid research in this species.
Influence of Authentic Leadership on Employees’ Taking Charge Behavior: The Roles of Subordinates’ Moqi and Perspective Taking
Authors: Wen Qiuxiang , Liu Ruhong , Long Jing
Published: May 2021

How to motivate employees to break through the role constraints and show more initiative determines the success or failure of a company’s future development. Taking charge behavior refers to the behavior where individuals influence the change of organizational function through voluntary and constructive efforts, which is a challenging organizational citizenship behavior. This study investigates the underlying mechanism and boundary condition of authentic leadership (AL) on employees’ taking charge behavior based on the role identity theory and literature concerning perspective taking. Matched data were collected from a multi-source sample that included 146 direct supervisors and 328 subordinates in mainland, China. The empirical results indicate that AL has a positive influence on the employees’ taking charge behavior, and subordinates’ moqi mediates the relationship between them. In addition, the employees’ perspective taking positively moderated the positive relationship between AL and subordinates’ moqi, as well as the mediating effect of subordinates’ moqi in the relationship between AL and employees’ taking charge behavior. Compared with the low levels of perspective taking, high levels of that made the influence of AL on subordinates’ moqi stronger, so is the whole indirect effect. This study is the first to explore the influencing mechanism of AL on employees’ taking charge behavior from the perspective of the role identity theory, thereby enriching the relevant studies and providing practical insights for organizational leaders regarding on how to foster employees to take charge.
Understanding the Factors Affecting Sustainable Energy Action Plan: A Case Study From the Covenant of Mayors Signatory Municipality in the Aegean Region of Turkey
Authors: Biresselioglu Mehmet Efe , Demir Muhittin Hakan
Published: May 2021

This study presents the case of a Metropolitan Municipality in the Aegean Region of Turkey, which undertook a series of initiatives to conduct projects on environmental protection and sustainability. This case study was conducted as two separate studies as a part of Horizon 2020-funded ECHOES project under Work Package 6, aiming to gain insight into the collective magnitudes of energy-related choices and behavior. The starting point of the process is marked, in 2015, by the municipality becoming a party to the Covenant of Mayors movement, joining around 8,000 signatories from over 50 countries. In line with European Union’s (EU’s) climate targets and associated energy-related policies, signatories of the Covenant of Mayors aim to decrease carbon emissions by 20% by 2020 and by 40% by 2030. In order to enhance the design and operationalization of policies for achieving these targets, each partner in the Covenant of Mayors is required to develop a Sustainable Energy Action Plan (SEAP). The SEAP is to be prepared within 2 years of becoming a party to the Covenant of Mayors and involves action plans and projects in order to operationalize the strategies for achieving the associated targets. To this end, this study analyzes the Metropolitan Municipality’s SEAP and its components, which include zero-emission public transportation project, transformation of existing buildings to a more energy-efficient standard and related energy audit studies, a project for decreasing waiting periods in traffic via a Smart Traffic System, a pedestrianization project, and a project for increasing the use of geothermal energy for district heating. This study set out to identify the internal and external factors, as well as bottom-up and top-down mechanisms involved in various phases of the preparation and implementation of the SEAP. The research method was expert interviews, incorporating viewpoints and perceptions of stakeholders from different levels of the municipality. Among the key results are understanding the roles of enthusiasts and frontrunners in such initiative and the importance of top-management and central government support.
State Transition Modeling in Ultimate Frisbee: Adaptation of a Promising Method for Performance Analysis in Invasion Sports
Authors: Lam Hilary , Kolbinger Otto , Lames Martin , Russomanno Tiago Guedes
Published: May 2021

Although the body of literature in sport science is growing rapidly, certain sports have yet to benefit from this increased interest by the scientific community. One such sport is Ultimate Frisbee, officially known as Ultimate. Thus, the goal of this study was to describe the nature of the sport by identifying differences between winning and losing teams in elite-level competition. To do so, a customized observational system and a state transition model were developed and applied to 14 games from the 2017 American Ultimate Disc League season. The results reveal that, on average, 262.2 passes were completed by a team per game and 5.5 passes per possession. More than two-thirds of these passes were played from the mid zone (39.4 ± 6.57%) and the rear zone (35.2 ± 5.09%), nearest the team’s own end zone. Winning and losing teams do not differ in these general patterns, but winning teams played significantly fewer backward passes from the front zone to the mid zone, nearest the opponent’s end zone than losing teams (mean difference of −4.73%, t(13) = −4.980, p < 0.001, d = −1.16). Furthermore, losing teams scored fewer points when they started on defense, called breakpoints (mean difference of −5.57, t(13) = −6.365, p < 0.001, d = 2.30), and committed significantly more turnovers per game (mean difference of 5.64, t(13) = 5.85, p < 0.001, d = −1.18). Overall, this study provides the first empirical description of Ultimate and identifies relevant performance indicators to discriminate between winning and losing teams. We hope this article sheds light on the unique, but so far overlooked sport of Ultimate, and offers performance analysts the basis for future studies using state transition modeling in Ultimate as well as other invasion sports.
Making Choices in Discourse: New Alternative Masculinities Opposing the “Warrior’s Rest”
Authors: Ruiz-Eugenio Laura , Toledo del Cerro Ana , Crowther Jim , Merodio Guiomar
Published: May 2021

Psychology research on men studies, attractiveness, and partner preferences has evolved from the influence of sociobiological perspectives to the role of interactions in shaping election toward sexual–affective relationships and desire toward different kinds of masculinities. However, there is a scientific gap in how language and communicative acts among women influence the kind of partner they feel attracted to and in the reproduction of relationship double standards, like the myth of the “warrior’s rest” where female attractiveness to “bad boys” is encouraged or supported. Some women imitate “the warrior” behavior of men by choosing dominant traditional masculinities (DTM) to have “fun” with and oppressed traditional masculinities (OTM) for “rest” after the “fun” with DTM—choosing an OTM for a stable relationship, but perhaps without passion, while also feeling attraction toward DTM, a response which perpetuates the chauvinist double standard that the feminist movement has condemned when men behave in this sexist way. Through conducting a qualitative study with communicative daily life stories, this article explores, on the one hand, how language and social interaction among women can lead to the reproduction of the DTM role by women and, on the other hand, also how new alternative masculinities (NAM) offer an alternative by explicitly rejecting, through the language of desire, to be the rest for the female warrior, the second fiddle to any woman. This has the potential to become a highly attractive alternative to DTM. Findings provide new knowledge through the analysis of communicative acts and masculinities evidencing the importance of language uses in the reproduction of the double standards in gender relations and to understand how and why these practices are maintained and which kind of language uses can contribute to preventing them. Implications for research and interventions on preventive socialization of gender violence are discussed.
Gaming Disorder and Well-Being Among Emirati College Women
Authors: Verlinden Marina , Thomas Justin , Almansoori Mahra Hasan Abdulla Ahamed , Wanigaratne Shamil
Published: May 2021

Background: The present study examined Internet Gaming Disorder (IGD) and depressive symptom levels among a predominantly female sample of college students from the United Arab Emirates (UAE).

Methods: IGD was assessed among two successive cohorts of students at the beginning of the academic year in 2016 and 2019, respectively. All participants (n = 412) completed the Internet Gaming Disorder Scale – Short-Form (IGDS9-SF) and the WHO-5 Well-being Index (WHO-5), a tool widely used for the screening and assessment of depressive symptomatology.

Results: Mean IGDS9-SF scores (15.85, SD = 6.40) were fairly similar to those observed in other nations. The prevalence of IGD was 1.45%, based on the stringent cut-off score (> = 40). Prevalence of IGD rose to 18.20% when using the less stringent cut-off (> = 21). There was an increase in the rate of IGD between 2016 and 2019, although not statistically significant. Higher IGDS9-SF scores were associated with greater depressive symptomatology; those scoring above the less stringent IGD cut-off had a greater likelihood of screening positive for depression OR = 2.28, 95% CI (1.176–4.428).

Conclusions: This study provides insights about IGD among a predominantly female Arab population, finding a correlation with mood disorder symptomatology and suggesting an increase in problematic gaming over time. The results are discussed with reference to the mood repair hypothesis and the possibility of IGD being a dual disorder. The association with depressive symptoms is also discussed in light of the neurobiology of addictive behaviors and sexual dimorphism.
Efficiency of an Optimized Care Organization in Fibromyalgia Patients: The From Intent to Move (FIMOUV) Study Protocol of a Randomized Controlled Trial
Authors: Colas Claire , Goutte Julie , Creac'h Christelle , Fontana Luc , Vericel Marie-Pierre , Manzanares Jessica , Peuriere Marie , Akrour Madjid , Martin Charly , Presles Emilie , Barth Nathalie , Guyot Jessica , Garros Maël , Trombert Béatrice , Massoubre Catherine , Roche Frédéric , Féasson Léonard , Marotte Hubert , Cathebras Pascal , Hupin David
Published: May 2021

Introduction: Fibromyalgia (FM) is characterized by multiple symptoms including pain, fatigue, and sleep disorders, altering patient's quality of life. In the absence of effective pharmacological therapy, the last European guidelines recommend a multidisciplinary management based on exercise and education. Thus, our main objective was to measure the effectiveness of a healthcare organization offering a specific program of adapted physical activity combined with a therapeutic education program for FM patients.

Methods and Analysis: The From Intent To Move (FIMOUV) study will recruit 330 FM patients randomized into two groups: test and control. The test group will benefit from a 1-month mixed exercise training program supervised at the hospital, followed by 2 months in a community-based relay in a health-sport structure. In addition, each of the two groups will benefit from therapeutic patient education sessions. The main endpoint is the measurement of the level of physical activity by accelerometry at 1 year. The secondary endpoints concern adherence to the practice of physical activity, impact on lifestyle, state of health, and physical capacity, as well as an estimate of the budgetary impact of this management strategy.

Discussion: This interventional research will allow us to assess the evolution of behaviors in physical activity after an FM syndrome management based solely on patient education or based on a supervised and adapted practice of physical activity associated with this same therapeutic education program. It seems to be the first study evaluating the impact of its intervention on objective data for measuring physical activity and sedentary behavior via accelerometry among FM patients.

Trial registration:ClinicalTrials.gov NCT04107948.
COVID-FACT: A Fully-Automated Capsule Network-Based Framework for Identification of COVID-19 Cases from Chest CT Scans
Authors: Heidarian Shahin , Afshar Parnian , Enshaei Nastaran , Naderkhani Farnoosh , Rafiee Moezedin Javad , Babaki Fard Faranak , Samimi Kaveh , Atashzar S. Farokh , Oikonomou Anastasia , Plataniotis Konstantinos N. , Mohammadi Arash
Published: May 2021

The newly discovered Coronavirus Disease 2019 (COVID-19) has been globally spreading and causing hundreds of thousands of deaths around the world as of its first emergence in late 2019. The rapid outbreak of this disease has overwhelmed health care infrastructures and arises the need to allocate medical equipment and resources more efficiently. The early diagnosis of this disease will lead to the rapid separation of COVID-19 and non-COVID cases, which will be helpful for health care authorities to optimize resource allocation plans and early prevention of the disease. In this regard, a growing number of studies are investigating the capability of deep learning for early diagnosis of COVID-19. Computed tomography (CT) scans have shown distinctive features and higher sensitivity compared to other diagnostic tests, in particular the current gold standard, i.e., the Reverse Transcription Polymerase Chain Reaction (RT-PCR) test. Current deep learning-based algorithms are mainly developed based on Convolutional Neural Networks (CNNs) to identify COVID-19 pneumonia cases. CNNs, however, require extensive data augmentation and large datasets to identify detailed spatial relations between image instances. Furthermore, existing algorithms utilizing CT scans, either extend slice-level predictions to patient-level ones using a simple thresholding mechanism or rely on a sophisticated infection segmentation to identify the disease. In this paper, we propose a two-stage fully automated CT-based framework for identification of COVID-19 positive cases referred to as the “COVID-FACT”. COVID-FACT utilizes Capsule Networks, as its main building blocks and is, therefore, capable of capturing spatial information. In particular, to make the proposed COVID-FACT independent from sophisticated segmentations of the area of infection, slices demonstrating infection are detected at the first stage and the second stage is responsible for classifying patients into COVID and non-COVID cases. COVID-FACT detects slices with infection, and identifies positive COVID-19 cases using an in-house CT scan dataset, containing COVID-19, community acquired pneumonia, and normal cases. Based on our experiments, COVID-FACT achieves an accuracy of 90.82%, a sensitivity of 94.55%, a specificity of 86.04%, and an Area Under the Curve (AUC) of 0.98, while depending on far less supervision and annotation, in comparison to its counterparts.
Modelling the Impact of Robotics on Infectious Spread Among Healthcare Workers
Authors: Vicente Raul , Mohamed Youssef , Eguíluz Victor M. , Zemmar Emal , Bayer Patrick , Neimat Joseph S. , Hernesniemi Juha , Nelson Bradley J. , Zemmar Ajmal
Published: May 2021

The Coronavirus disease 2019 (Covid-19) pandemic has brought the world to a standstill. Healthcare systems are critical to maintain during pandemics, however, providing service to sick patients has posed a hazard to frontline healthcare workers (HCW) and particularly those caring for elderly patients. Various approaches are investigated to improve safety for HCW and patients. One promising avenue is the use of robots. Here, we model infectious spread based on real spatio-temporal precise personal interactions from a geriatric unit and test different scenarios of robotic integration. We find a significant mitigation of contamination rates when robots specifically replace a moderate fraction of high-risk healthcare workers, who have a high number of contacts with patients and other HCW. While the impact of robotic integration is significant across a range of reproductive number R0, the largest effect is seen when R0 is slightly above its critical value. Our analysis suggests that a moderate-sized robotic integration can represent an effective measure to significantly reduce the spread of pathogens with Covid-19 transmission characteristics in a small hospital unit.
Perceived Time, Frequency, and Intensity of Engagement and Older Masters Athletes' Subjective Experiences
Authors: Deck Sarah , Doherty Alison , Hall Craig , Schneider Angela , Patil Swarali , Belfry Glen
Published: May 2021

Masters athletes are a unique group of older adults whose experiences may provide valuable insights into the role of sport for successful aging. The purpose of this study was to explore whether masters athletes' social and psychological experiences vary with their time, frequency, and perceived exertion in training and competition. Semi-structured interviews were conducted with 40 men and women older masters athletes, aged 50–79 years (M = 66), who were active at the competitive level across a variety of sports (e.g., volleyball, curling, rowing, dragon boating, running, swimming, and basketball) at the time of the study. Results indicate that all participants experienced social and psychological benefits from engaging in masters sport. Only the high-frequency engagement subgroup (participating five to seven times per week in training and/or competition) reported social downsides, in terms of missing time with family and friends outside of masters sport. However, some participants described the positive family support (e.g., spouse who endorses sport participation) that overrides some of the social costs. These findings have implications for realizing positive experiences with minimal engagement in masters sport, yet an apparent threshold of participation beyond which negative social consequences may be experienced. This is an important consideration for the design and promotion of sport for older adults.
Case Report: Complicated Meckel Diverticulum Spectrum in Children
Authors: Gunadi  , Damayanti Wahyu , Saputra Robin Perdana , Ramadhita  , Ibrohim Ibnu Sina , Lestiono Andi , Melati Devy , Permatahati Winda Intan , Widowati Titis , Makhmudi Akhmad
Published: May 2021

Background: Meckel diverticulum (MD) is the most common congenital anomaly of the intestines, with an incidence of 2% of the general population. It can present as various clinical features with complications and be life threatening if diagnosis is delayed and treatment late.

Case Presentation: We report three pediatric cases with complicated MD: one female presented with small-bowel obstruction, one male with peritonitis, and one female with severe iron-deficiency anemia, without gross gastrointestinal bleeding nor any ectopic gastric mucosa. All patients underwent exploratory laparotomy, segmental small-bowel resection, and primary anastomosis. They successfully recovered and were uneventfully discharged on the fourth, seventh, and 10th postoperative days, respectively.

Conclusions: MD can present with various complication spectrums, including small-bowel obstruction, peritonitis, and severe iron-deficiency anemia, which may cause difficulty in definitive diagnosis, particularly in children. Segmental small-bowel resection and primary anastomosis are effective surgical approaches and show good outcomes for MD patients.
Integrating miRNA and mRNA Profiling to Assess the Potential miRNA–mRNA Modules Linked With Testicular Immune Homeostasis in Sheep
Authors: Li Taotao , Wang Xia , Luo Ruirui , An Xuejiao , Zhang Yong , Zhao Xingxu , Ma Youji
Published: May 2021

Beyond its well-known role in spermatogenesis and androgen production, mammalian testes are increasingly recognized as an immune-privileged organ for protecting autoantigenic germ cells, especially meiotic and postmeiotic germ cells, from systemic immune responses. Despite its importance, the molecular mechanisms underlying this regulation in mammals, including sheep, are far from known. In this study, we searched for the genes associated with testicular immune privilege and assessed their possible modulating mechanisms by analyzing systematic profiling of mRNAs and miRNAs on testicular tissues derived from prepubertal and postpubertal Tibetan sheep acquired by RNA sequencing. We identified 1,118 differentially expressed (DE) mRNAs associated with immunity (245 increased mRNAs and 873 decreased mRNAs) and 715 DE miRNAs (561 increased miRNAs and 154 decreased miRNAs) in postpubertal testes compared with prepuberty. qPCR validations for 20 DE mRNAs and 16 miRNAs showed that the RNA-seq results are reliable. By using Western blot, the postpubertal testes exhibited decreased protein abundance of CD19 and TGFBR2 (two proteins encoded by DE mRNAs) when compared with prepuberty, consistent with mRNA levels. The subsequent immunofluorescent staining showed that the positive signals for the CD19 protein were observed mainly in Sertoli cells and the basement membrane of pre- and postpubertal testes, as well as the prepubertal testicular vascular endothelium. The TGFBR2 protein was found mostly in interstitial cells and germ cells of pre- and postpubertal testes. Functional enrichment analysis indicated that DE mRNAs were mainly enriched in biological processes or pathways strongly associated with the blood–testis barrier (BTB) function. Many decreased mRNAs with low expression abundance were significantly enriched in pathways related to immune response. Also, multiple key miRNA-target negative correlation regulatory networks were subsequently established. Furthermore, we verified the target associations between either oar-miR-29b or oar-miR-1185-3p and ITGB1 by dual-luciferase reporter assay. Finally, a putative schematic model of the miRNA-mRNA-pathway network mediated by immune homeostasis-related genes was proposed to show their potential regulatory roles in sheep testicular privilege. Taken together, we conclude that many immune-related genes identified in this study are negatively regulated by potential miRNAs to participate in the homeostatic regulation of testicular immune privilege of sheep by sustaining BTB function and inhibiting immune responses under normal physiological conditions. This work offers the first global view of the expression profiles of miRNAs/mRNAs involved in sheep testicular immune privilege and how the genes potentially contribute to immune-homeostatic maintenance.
Decadal and Bi-Decadal Periodicities in Temperature of Southern Scandinavia: Manifestations of Natural Variability or Climatic Response to Solar Cycles?
Authors: Ogurtsov Maxim
Published: May 2021

Nine proxies of temperature over the last 225–300 years in Southern Fennoscandia (55–63° N) were analyzed. Seven reconstructions of the mean growing season temperatures were obtained by dendroclimatological methods. Reconstructions of spring temperatures in Stockholm and winter temperatures in Tallinn were based on historical documentary sources. It was found that significant decadal (10–13 years) and bi-decadal (22–25 years) periodicities were present in many of these series during the entire time interval. Four proxy records correlated significantly with the quasi 22-year solar cycle of Hale. Three time series correlated significantly with the quasi 11-year solar cycle of Schwabe. This can be considered as evidence of a link between decadal and bi-decadal changes in solar activity and climate in Southern Fennoscandia. On the other hand, signs of correlation differed, as well as the time shift between the solar and temperature cycles. It is difficult to explain such an intricate relationship and, thus, the physical mechanism of solar−climatic linkages remains unclear. That is why assumptions about the purely occasional appearance of correlations cannot yet be rejected. Guidelines for further research are suggested.
Colorectal Cancer Anatomical Site and Sleep Quality
Authors: Ton Mimi , Watson Nathaniel F. , Sillah Arthur , Malen Rachel C. , Labadie Julia D. , Reedy Adriana M. , Cohen Stacey A. , Burnett-Hartman Andrea N. , Newcomb Polly A. , Phipps Amanda I.
Published: May 2021

Purpose: Sleep quality in relation to anatomic site among colorectal cancer (CRC) patients is not well understood, though discerning the relationship could contribute to improved survivorship care. Methods: We ascertained sleep quality (Pittsburgh Sleep Quality Index) and other personal characteristics within an ongoing population-based study of CRC patients identified through a cancer registry (N = 1453). Differences in sleep quality by CRC site were analyzed using chi-square and ANOVA tests. We used logistic regression to estimate odds ratios (ORs) and 95% confidence intervals (CIs) for the association of tumor site with sleep quality concerns, adjusting for patient attributes and time since diagnosis. Results: Sleeping problems were reported by 70% of CRC patients. Overall, participants with rectal (vs. colon) cancer were more likely (OR (95% CI)) to report general trouble sleeping (1.58 (1.19, 2.10)). Rectal cancer patients were also more likely than colon cancer patients to report changes in sleep patterns after cancer diagnosis (1.38 (1.05, 1.80)), and trouble sleeping specifically due to getting up to use the bathroom (1.53 (1.20, 1.96)) or pain (1.58 (1.15, 2.17)), but were less likely to report trouble sleeping specifically due to issues with breathing/coughing/snoring (0.51 (0.27, 0.99)). Conclusion: Overall, rectal cancer patients were more likely to have sleep complications compared to colon cancer patients. This suggests sleep-focused survivorship care may be adapted according to CRC site to ensure patients receive appropriate support.
An Example of Personalized Treatment in HR+ HER2+ Long Survivor Breast Cancer Patient (Case Report)
Authors: Panebianco Martina , Taurelli Salimbeni Beatrice , Roberto Michela , Marchetti Paolo
Published: May 2021

Background. Personalized therapy is becoming increasingly popular in oncological scenarios, not only based on molecular pharmacological targets, but also preventing any drug–drug–gene interaction (DDGI), which could lead to severe toxicities. Single nucleotide polymorphisms (SNPs), the individual germline sequence variations in genes involved in drug metabolism, are correlated to interindividual response to drugs and explain both efficacy and toxicity profiles reported by patients. Case presentation. We present the case of a woman suffering from triple-positive breast cancer; she had early-stage disease at the onset and after four years developed metastatic disease. During her history, she presented different toxicities due to antineoplastic treatments. Particularly, hypertransaminasemia was found during every line of treatment. Nevertheless, we were able to guarantee the patient an excellent therapeutic adhesion thanks to the supportive treatments and the reduction of drug dosage. Moreover, we conducted a simultaneous analysis of the patient’s biochemical and genomic data thanks to Drug-PIN software, and we found several significant SNPs of the main enzymes and transporters involved in drug metabolism. Conclusion. Our case report demonstrated the relevance of DDGI in clinical practice management of a patient treated for advanced breast cancer, suggesting the role of Drug-PIN software as an easy-to-use tool to prevent adverse events during cancer treatment and to help physicians in therapeutic algorithms. However, further studies are needed to confirm these results.
Entropy and Multifractal-Multiscale Indices of Heart Rate Time Series to Evaluate Intricate Cognitive-Autonomic Interactions
Authors: Bouny Pierre , Arsac Laurent M. , Touré Cuq Emma , Deschodt-Arsac Veronique
Published: May 2021

Recent research has clarified the existence of a networked system involving a cortical and subcortical circuitry regulating both cognition and cardiac autonomic control, which is dynamically organized as a function of cognitive demand. The main interactions span multiple temporal and spatial scales and are extensively governed by nonlinear processes. Hence, entropy and (multi)fractality in heart period time series are suitable to capture emergent behavior of the cognitive-autonomic network coordination. This study investigated how entropy and multifractal-multiscale analyses could depict specific cognitive-autonomic architectures reflected in the heart rate dynamics when students performed selective inhibition tasks. The participants (N=37) completed cognitive interference (Stroop color and word task), action cancellation (stop-signal) and action restraint (go/no-go) tasks, compared to watching a neutral movie as baseline. Entropy and fractal markers (respectively, the refined composite multiscale entropy and multifractal-multiscale detrended fluctuation analysis) outperformed other time-domain and frequency-domain markers of the heart rate variability in distinguishing cognitive tasks. Crucially, the entropy increased selectively during cognitive interference and the multifractality increased during action cancellation. An interpretative hypothesis is that cognitive interference elicited a greater richness in interactive processes that form the central autonomic network while action cancellation, which is achieved via biasing a sensorimotor network, could lead to a scale-specific heightening of multifractal behavior.
Shall I Work with Them? A Knowledge Graph-Based Approach for Predicting Future Research Collaborations
Authors: Kanakaris Nikos , Giarelis Nikolaos , Siachos Ilias , Karacapilidis Nikos
Published: May 2021

We consider the prediction of future research collaborations as a link prediction problem applied on a scientific knowledge graph. To the best of our knowledge, this is the first work on the prediction of future research collaborations that combines structural and textual information of a scientific knowledge graph through a purposeful integration of graph algorithms and natural language processing techniques. Our work: (i) investigates whether the integration of unstructured textual data into a single knowledge graph affects the performance of a link prediction model, (ii) studies the effect of previously proposed graph kernels based approaches on the performance of an ML model, as far as the link prediction problem is concerned, and (iii) proposes a three-phase pipeline that enables the exploitation of structural and textual information, as well as of pre-trained word embeddings. We benchmark the proposed approach against classical link prediction algorithms using accuracy, recall, and precision as our performance metrics. Finally, we empirically test our approach through various feature combinations with respect to the link prediction problem. Our experimentations with the new COVID-19 Open Research Dataset demonstrate a significant improvement of the abovementioned performance metrics in the prediction of future research collaborations.
Count Data Time Series Modelling in Julia—The CountTimeSeries.jl Package and Applications
Authors: Stapper Manuel
Published: May 2021

A new software package for the Julia language, CountTimeSeries.jl, is under review, which provides likelihood based methods for integer-valued time series. The package’s functionalities are showcased in a simulation study on finite sample properties of Maximum Likelihood (ML) estimation and three real-life data applications. First, the number of newly infected COVID-19 patients is predicted. Then, previous findings on the need for overdispersion and zero inflation are reviewed in an application on animal submissions in New Zealand. Further, information criteria are used for model selection to investigate patterns in corporate insolvencies in Rhineland-Palatinate. Theoretical background and implementation details are described, and complete code for all applications is provided online. The CountTimeSeries package is available at the general Julia package registry.
Solid-State Electrochemical Energy Storage Based on Soluble Melanin
Authors: Paulin João V. , Fernandes Silvia L. , Graeff Carlos F. O.
Published: May 2021

Biocompatible and biodegradable powering materials are appealing systems for biomedical and electronic devices. Melanin is a natural and multifunctional material with redox capability, which is of great interest in electrochemical energy storage functionalities. In our work, we explored the use of soluble melanin derivatives as active materials for symmetric solid-state supercapacitors operating in the dark and under illumination. We observed that our devices were photo-pseudocapacitive. Additionally, under illumination, our best device showed a specific capacitance of 57.7 mFg−1 at a scan rate of 0.01 Vs−1, with a decrease of 53% in resistance compared to that in the dark. Our outcome suggests that soluble melanin is a promising material for solid-state powering elements in wearable and environmentally friendly devices.
Location of Biorefineries Based on Olive-Derived Biomass in Andalusia, Spain
Authors: Cardoza Diego , Romero Inmaculada , Martínez Teresa , Ruiz Encarnación , Gallego Francisco J. , López-Linares Juan Carlos , Manzanares Paloma , Castro Eulogio
Published: May 2021

A biorefinery integrated process based on lignocellulosic feedstock is especially interesting in rural areas with a high density of agricultural and agro-industrial wastes, which is the case for olive crop areas and their associated industries. In the region of Andalusia, in the south of Spain, the provinces of Jaén, Córdoba and Seville accumulate more than 70% of the olive wastes generated in Spain. Therefore, the valorisation of these wastes is a matter of interest from both an environmental and a social point of view. The olive biorefinery involves a multi-product process from different raw materials: olive leaves, exhausted olive pomace, olive stones and olive tree pruning residues. Biorefinery processes associated with these wastes would allow their valorisation to produce bioenergy and high value-added renewable products. In this work, using geographic information system tools, the biomass from olive crop fields, mills and olive pomace-extracting industries, where these wastes are generated, was determined and quantified in the study area. In addition, the vulnerability of the territory was evaluated through an environmental and territorial analysis that allowed for the determination of the reception capacity of the study area. Then, information layers corresponding to the availability of the four biomass wastes, and layers corresponding to the environmental fragility of the study area were overlapped and they resulted in an overall map. This made it possible to identify the best areas for the implementation of the biorefineries based on olive-derived biomass. Finally, as an example, three zones were selected for this purpose. These locations corresponded to low fragility areas with a high availability of biomass (more than 300,000 tons/year) in a 30 km radius, which would ensure the biomass supply.
Pore Type, Pore Structure, and Controlling Factors in the Late Triassic Lacustrine Yanchang Shale, Ordos Basin, China
Authors: Cheng Ming , Lei Yuhong , Luo Xiaorong , Zhang Likuan , Wang Xiangzeng , Zhang Lixia , Jiang Chengfu , Yin Jintao
Published: May 2021

Organic-rich lacustrine shales in the Upper Triassic Yanchang Formation with thermal maturity mainly in the oil window are the main shale oil and shale gas system in the lacustrine strata of the Ordos Basin, China. Pore systems are important for the storage and transfer of shale oil and gas. The main objectives of this study are to identify the pore types and pore structures and investigate the controlling factors for pore types, pore structures, and total porosities of the lacustrine Yanchang Shale. In this study, organic-rich mudstones, mudstones with siltstone interlayers, siltstone, and sandstones were selected from 15 wells in the southern Ordos Basin. X-ray diffraction, pyrolysis, scanning electron microscopy (SEM), low-pressure nitrogen adsorption analysis, and helium porosimetry were conducted to investigate the mineral compositions, pore types, pore structures, porosities, and controlling factors. Siltstone and sandstone interlayers heterogeneously developed in the Yanchang Shale. The petrology, mineral composition, geochemistry, pore type, pore structure, and porosity of siltstone interlayers are different from those of mudstones. The siltstone and sandstone interlayers usually have more quartz and feldspars, greater detrital grain sizes, and relatively better grain sorting but are lower in clay minerals, total organic carbon (TOC), amount of free liquid hydrocarbons values (S1), and total residual hydrocarbons values (S2), compared to mudstones. Interparticle (interP), intraparticle (intraP) pores, and organic pores (OPs) were developed in both siltstones and mudstones. OPs were observed in samples with lower thermal maturity (e.g., 0.5–0.85%). The inorganic pore size is greater than that of OPs. Additionally, the inorganic pore diameters in siltstone interlayers are also greater than those in mudstones. Organic-rich mudstones generally have higher pore volumes (PVs) of pores with sizes less than 10 nm, pore volumes of pores with sizes between 10 and 50 nm (PV, 10–50 nm), and specific surface area (SSA), but they have lower PVs of pores with sizes greater than 50 nm, total PV, and porosity when compared to siltstone and sandstone interlayers. The dominant pore type in mudstones is OPs and TOC (first order), sources and OM types (second order), and thermal maturity (third order), while the abundances of rigid grains with greater sizes and grain sorting are the main controlling factors of pore structures, SSA and PV. Both inorganic pores and organic pores are abundant in the siltstone interlayers. The pore size distribution (PSD), PV, and porosity of siltstone interlayers are related to the abundance of rigid grains (first order), grain sorting (second order), grain size (third order), and carbonate cement content. The total PV and porosity of Yanchang Shale reservoirs may have increased with the increased abundance of siltstone and sandstone interlayers.