bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Hybrid Wind-Wave Energy Converter for Jacket-Frame Substructures
Authors: Perez-Collazo Carlos , Greaves Deborah , Iglesias Gregorio
Published: March 2018

The growth of the offshore wind industry in the last couple of decades has made this technology a key player in the maritime sector. The sustainable development of the offshore wind sector is crucial for this to consolidate within a global scenario of climate change and increasing threats to the marine environment. In this context, multipurpose platforms have been proposed as a sustainable approach to harnessing different marine resources and combining their use under the same platform. Hybrid wind-wave systems are a type of multipurpose platform where a single platform combines the exploitation of offshore wind and wave energy. In particular, this paper deals with a novel hybrid wind-wave system that integrates an oscillating water column wave energy converter with an offshore wind turbine on a jacket-frame substructure. The main objective of this paper is to characterise the hydrodynamic response of the WEC sub-system of this hybrid energy converter. A 1:50 scale model was tested under regular and irregular waves to characterise the hydrodynamic response of the WEC sub-system. The results from this analysis lead to the proof of concept of this novel hybrid system; but additionally, to characterising its behaviour and interaction with the wave field, which is a requirement for fully understanding the benefits of hybrid systems.
Comparison and Optimization of Saccharification Conditions of Alkaline Pre-Treated Triticale Straw for Acid and Enzymatic Hydrolysis Followed by Ethanol Fermentation
Authors: Łukajtis Rafał , Kucharska Karolina , Hołowacz Iwona , Rybarczyk Piotr , Wychodnik Katarzyna , Słupek Edyta , Nowak Paulina , Kamiński Marian
Published: March 2018

This paper concerns the comparison of the efficiency of two-stage hydrolysis processes, i.e., alkaline pre-treatment and acid hydrolysis, as well as alkaline pre-treatment followed by enzymatic hydrolysis, carried out in order to obtain reducing sugars from triticale straw. For each of the analyzed systems, the optimization of the processing conditions was carried out with respect to the glucose yield. For the alkaline pre-treatment, an optimal catalyst concentration was selected for constant values of temperature and pre-treatment time. For enzymatic hydrolysis, optimal process time and concentration of the enzyme preparation were determined. For the acidic hydrolysis, performed with 85% phosphoric acid, the optimum temperature and hydrolysis time were determined. In the hydrolysates obtained after the two-stage treatment, the concentration of reducing sugars was determined using HPLC. The obtained hydrolysates were subjected to ethanol fermentation. The concentrations of fermentation inhibitors are given and their effects on the alcoholic fermentation efficiency are discussed.
Role of Community and User Attributes in Collective Action: Case Study of Community-Based Forest Management in Nepal
Authors: Negi Swati , Pham Thu , Karky Bhaskar , Garcia Claude
Published: March 2018

A growing literature on collective action focuses on exploring the conditions that might help or hinder groups to work collectively. In this paper, we focus on community-based forest management in the inner Terai region of Nepal and explore the role of community and user attributes such as group size, social heterogeneities, forest user’ perception on forests, and affiliation to the user group, in the collective action of managing community forests. Household surveys were carried out with 180 households across twelve community forest users’ groups. We first measured ethnic diversity, income inequality, landholding inequality, and user perception towards the use and management of community forests to understand their effect on the participation of forest users in the management of community forests. Our results show that among the studied variables, group size (number of forest users affiliated to the community forests) and perception of the management of their community forests are strong predictors of forest user participation in community forest management. Income inequality and ethnic diversity were found to have no significant association. Land inequality, however, was found to decrease participation in the management and use of community forests. These community and user attributes play a crucial role in the success of collective action and may vary from community to community. Hence they need to be duly considered by the practitioners prior to any community-based project interventions for stimulating successful collective action.
Multi-Wavelength Polarimetry of Isolated Neutron Stars
Authors: Mignani Roberto
Published: March 2018

Isolated neutron stars are known to be endowed with extreme magnetic fields, whose maximum intensity ranges from 10 12 – 10 15 G, which permeates their magnetospheres. Their surrounding environment is also strongly magnetized, especially in the compact nebulae powered by the relativistic wind from young neutron stars. The radiation from isolated neutron stars and their surrounding nebulae is, thus, supposed to bring a strong polarization signature. Measuring the neutron star polarization brings important information about the properties of their magnetosphere and of their highly magnetized environment. Being the most numerous class of isolated neutron stars, polarization measurements have been traditionally carried out for radio pulsars, hence in the radio band. In this review, I summarize multi-wavelength linear polarization measurements obtained at wavelengths other than radio both for pulsars and other types of isolated neutron stars and outline future perspectives with the upcoming observing facilities.
Testing of Alignment Parameters for Ancient Samples: Evaluating and Optimizing Mapping Parameters for Ancient Samples Using the TAPAS Tool
Authors: Taron Ulrike , Lell Moritz , Barlow Axel , Paijmans Johanna
Published: March 2018

High-throughput sequence data retrieved from ancient or other degraded samples has led to unprecedented insights into the evolutionary history of many species, but the analysis of such sequences also poses specific computational challenges. The most commonly used approach involves mapping sequence reads to a reference genome. However, this process becomes increasingly challenging with an elevated genetic distance between target and reference or with the presence of contaminant sequences with high sequence similarity to the target species. The evaluation and testing of mapping efficiency and stringency are thus paramount for the reliable identification and analysis of ancient sequences. In this paper, we present ‘TAPAS’, (Testing of Alignment Parameters for Ancient Samples), a computational tool that enables the systematic testing of mapping tools for ancient data by simulating sequence data reflecting the properties of an ancient dataset and performing test runs using the mapping software and parameter settings of interest. We showcase TAPAS by using it to assess and improve mapping strategy for a degraded sample from a banded linsang (Prionodon linsang), for which no closely related reference is currently available. This enables a 1.8-fold increase of the number of mapped reads without sacrificing mapping specificity. The increase of mapped reads effectively reduces the need for additional sequencing, thus making more economical use of time, resources, and sample material.
Alternative Splicing in the Hippo Pathway—Implications for Disease and Potential Therapeutic Targets
Authors: Porazinski Sean , Ladomery Michael
Published: March 2018

Alternative splicing is a well-studied gene regulatory mechanism that produces biological diversity by allowing the production of multiple protein isoforms from a single gene. An involvement of alternative splicing in the key biological signalling Hippo pathway is emerging and offers new therapeutic avenues. This review discusses examples of alternative splicing in the Hippo pathway, how deregulation of these processes may contribute to disease and whether these processes offer new potential therapeutic targets.
Respiratory Health Symptoms among Schoolchildren in Relation to Possible Food-Related Risk and Protective Factors
Authors: Wright Caradee , Nkosi Vusumuzi , Wichmann Janine
Published: March 2018

Respiratory health outcomes are among the top five causes of child morbidity and mortality around the world. We aimed to investigate possible food-related risk and protective factors for respiratory health outcomes in children. Structured questionnaires completed by primary caregivers of 10-year old children were used to collect information on demographics, socio-economic status, house characteristics and child respiratory health status. Upper (URIs) and Lower (LRIs) respiratory illnesses comprised hay fever, and wheezing, asthma and bronchitis, respectively. Eight hundred questionnaires were distributed, 648 retrieved and 420 completed in full (52.5% response rate). The hay fever 6-month prevalence was 22.4% and wheezing had the highest 6-month prevalence among the LRIs (13.8%). The majority of children ate vegetables (75.5%), fruit (69.3%) and chicken or fish (81.7%) regularly. Nearly half of the children (45.5%) regularly ate processed food. Eating processed food regularly was statistical significantly associated with wheeze (Adjusted Odds Ratio (OR) = 2.65; 95% CI: 1.38–5.08), hay fever (OR = 1.62; 95% CI: 1.09–2.64) and bronchitis (OR = 1.27; 95% CI: 1.06–2.56). The study found an association between regular consumption of processed foods and wheeze, hay fever and bronchitis among 10 year old children. The regular consumption of processed food plays a role in adverse respiratory health effects among children and healthy eating is emphasized.
A Systematic Underpinning and Framing of the Servicescape: Reflections on Future Challenges in Healthcare Services
Authors: Han Jieun , Kang Hyo-Jin , Kwon Gyu
Published: March 2018

Ever since Bitner defined the term “servicescape” as the physical environment in which the service is assembled, several scholars have attempted to better understand the impact of the built environment in the context of different service settings. While servicescape is a topic of increasing academic interest among scholars and practitioners, most studies in the area are dedicated to understanding the built environment of hedonic service. More studies are needed to examine utilitarian servicescape and in this paper, we have focused on the healthcare environment. This study aims to identify the gap in servicescape and healthscape studies by providing a theoretical structure of the current servicescape literature and comprehend the academic differences between hedonic servicescape and utilitarian healthscape studies. After reviewing 44 selected papers based on rigorous criteria, we: (1) framed the servicescape factors; (2) analyzed the servicescape literature from the perspectives of terminologies, research fields, methodologies, and frameworks; and (3) identified the current paths of healthscape research. Through this work, we highlight the significance of adopting different dimensions and factors to evaluate the distinguished service environment by the servicescape type and propose several research agendas for future studies on healthscapes. The research findings can contribute to a deep understanding of healthscapes and can introduce a new viewpoint for interpreting the servicescape in diversified service settings.
High NOTCH1 mRNA Expression Is Associated with Better Survival in HNSCC
Authors: Wirth Markus , Jira Daniel , Ott Armin , Piontek Guido , Pickhard Anja
Published: March 2018

The clinical impact of the expression of NOTCH1 signaling components in squamous cell carcinoma of the pharynx and larynx has only been evaluated in subgroups. The aim of this study was therefore to evaluate NOTCH1 expression in head and neck squamous cell cancer (HNSCC) patient tissue and cell lines. We analyzed tissue from 195 HNSCCs and tissue from 30 normal patients for mRNA expression of NOTCH1, NOTCH3, HES1, HEY1, and JAG1 using quantitative real-time PCR. Association of expression results and clinical orpathological factors was examined with multivariate Cox regression. NOTCH1 expression was determined in three Human Papilloma Virus (HPV)-positive and nine HPV-negative HNSCC cell lines. High expression of NOTCH1 was associated with better overall survival (p = 0.013) and disease-free survival (p = 0.040). Multivariate Cox regression confirmed the significant influence of NOTCH1 expression on overall survival (p = 0.033) and disease-free survival (p = 0.029). A significant correlation was found between p16 staining and NOTCH1 mRNA expression (correlation coefficient 0.28; p = 0.01). NOTCH1 was expressed at higher levels in HPV-positive HNSCC cell lines compared with HPV-negative cell lines, which was not statistically significant (p = 0.068). We conclude that NOTCH1 expression is associated with overall survival, and that inhibition of NOTCH1 therefore seems less promising.
The Role of Macrophages in Neuroinflammatory and Neurodegenerative Pathways of Alzheimer’s Disease, Amyotrophic Lateral Sclerosis, and Multiple Sclerosis: Pathogenetic Cellular Effectors and Potential Therapeutic Targets
Authors: Mammana Santa , Fagone Paolo , Cavalli Eugenio , Basile Maria , Petralia Maria , Nicoletti Ferdinando , Bramanti Placido , Mazzon Emanuela
Published: March 2018

In physiological conditions, different types of macrophages can be found within the central nervous system (CNS), i.e., microglia, meningeal macrophages, and perivascular (blood-brain barrier) and choroid plexus (blood-cerebrospinal fluid barrier) macrophages. Microglia and tissue-resident macrophages, as well as blood-borne monocytes, have different origins, as the former derive from yolk sac erythromyeloid precursors and the latter from the fetal liver or bone marrow. Accordingly, specific phenotypic patterns characterize each population. These cells function to maintain homeostasis and are directly involved in the development and resolution of neuroinflammatory processes. Also, following inflammation, circulating monocytes can be recruited and enter the CNS, therefore contributing to brain pathology. These cell populations have now been identified as key players in CNS pathology, including autoimmune diseases, such as multiple sclerosis, and degenerative diseases, such as Amyotrophic Lateral Sclerosis and Alzheimer’s disease. Here, we review the evidence on the involvement of CNS macrophages in neuroinflammation and the advantages, pitfalls, and translational opportunities of pharmacological interventions targeting these heterogeneous cellular populations for the treatment of brain diseases.
Investigation of Differences in Fertility among Progenies from Self-Pollinated Chrysanthemum
Authors: Wang Fan , Zhong Xinghua , Wang Haibin , Song Aiping , Chen Fadi , Fang Weimin , Jiang Jiafu , Teng Nianjun
Published: March 2018

Most chrysanthemum cultivars are self-incompatible, so it is very difficult to create pure lines that are important in chrysanthemum breeding and theoretical studies. In our previous study, we obtained a self-compatible chrysanthemum cultivar and its self-pollinated seed set was 56.50%. It was interesting that the seed set of its ten progenies ranged from 0% to 37.23%. Examination of the factors causing the differences in the seed set will lead to an improved understanding of chrysanthemum self-incompatibility, and provide valuable information for creating pure lines. Pollen morphology, pollen germination percentage, pistil receptivity and embryo development were investigated using the in vitro culture method, the paraffin section technique, scanning electron microscopy and transmission electron microscopy. Moreover, RNA sequencing and bioinformatics were applied to analyzing the transcriptomic profiles of mature stigmas and anthers. It was found that the self-pollinated seed set of “Q10-33-1①”,”Q10-33-1③”,”Q10-33-1④” and “Q10-33-1⑩” were 37.23%, 26.77%, 7.97% and 0%, respectively. The differences in fertility among four progenies were mainly attributable to differences in pollen germination percentage and pistil receptivity. Failure of the seed set in “Q10-33-1⑩” was possibly due to self-incompatibility. In the transcriptomic files, 22 potential stigma S genes and 8 potential pollen S genes were found out.
Cannabinoid Receptors and the Endocannabinoid System: Signaling and Function in the Central Nervous System
Authors: Zou Shenglong , Kumar Ujendra
Published: March 2018

The biological effects of cannabinoids, the major constituents of the ancient medicinal plant Cannabis sativa (marijuana) are mediated by two members of the G-protein coupled receptor family, cannabinoid receptors 1 (CB1R) and 2. The CB1R is the prominent subtype in the central nervous system (CNS) and has drawn great attention as a potential therapeutic avenue in several pathological conditions, including neuropsychological disorders and neurodegenerative diseases. Furthermore, cannabinoids also modulate signal transduction pathways and exert profound effects at peripheral sites. Although cannabinoids have therapeutic potential, their psychoactive effects have largely limited their use in clinical practice. In this review, we briefly summarized our knowledge of cannabinoids and the endocannabinoid system, focusing on the CB1R and the CNS, with emphasis on recent breakthroughs in the field. We aim to define several potential roles of cannabinoid receptors in the modulation of signaling pathways and in association with several pathophysiological conditions. We believe that the therapeutic significance of cannabinoids is masked by the adverse effects and here alternative strategies are discussed to take therapeutic advantage of cannabinoids.
Copper-Free ‘Click’ Chemistry-Based Synthesis and Characterization of Carbonic Anhydrase-IX Anchored Albumin-Paclitaxel Nanoparticles for Targeting Tumor Hypoxia
Authors: Tatiparti Katyayani , Sau Samaresh , Gawde Kaustubh , Iyer Arun
Published: March 2018

Triple negative breast cancer (TNBC) is a difficult to treat disease due to the absence of the three unique receptors estrogen, progesterone and herceptin-2 (HER-2). To improve the current therapy and overcome the resistance of TNBC, there is unmet need to develop an effective targeted therapy. In this regard, one of the logical and economical approaches is to develop a tumor hypoxia-targeting drug formulation platform for selective delivery of payload to the drug-resistant and invasive cell population of TNBC tumors. Toward this, we developed a Carbonic Anhydrase IX (CA IX) receptor targeting human serum albumin (HSA) carriers to deliver the potent anticancer drug, Paclitaxel (PTX). We used Acetazolamide (ATZ), a small molecule ligand of CA IX to selectively deliver HSA-PTX in TNBC cells. A novel method of synthesis involving copper free ‘click’ chemistry (Dibenzocyclooctyl, DBCO) moiety with an azide-labeled reaction partner, known as Strain-Promoted Alkyne Azide Cycloaddition (SPAAC) along with a desolvation method for PTX loading were used in the present study to arrive at the CA IX selective nano-carriers, HSA-PTX-ATZ. The anticancer effect of HSA-PTX-ATZ is higher compared to HSA, PTX and non-targeted HSA-PTX in MDA-MB-231 and MDA-MB-468 cells. The cell killing effect is associated with induction of early and late phases of apoptosis. Overall, our proof-of-concept study shows a promising avenue for hypoxia-targeted drug delivery that can be adapted to several types of cancers.
In Vitro Cocktail Effects of PCB-DL (PCB118) and Bulky PCB (PCB153) with BaP on Adipogenesis and on Expression of Genes Involved in the Establishment of a Pro-Inflammatory State
Authors: May Phealay , Bremond Patricia , Sauzet Christophe , Piccerelle Philippe , Grimaldi Frédérique , Champion Serge , Villard Pierre-Henri
Published: March 2018

(1) Objective: Highlight the in vitro effects of 3T3-L1 cell exposure to polychlorinated biphenyls (PCB118 and 153) or benzo(a)pyrene (BaP) alone or as a cocktail on adipogenesis (ADG) by focusing on changes in lipid metabolism and inflammatory-related genes expression (INFG) and ADG-related genes expression (ADGG); (2) Results: Treatment from the early stage of cell differentiation by BaP alone or in combination with PCBs decreased the expression of some of the ADGG (PPARγ Glut-4, FAS, Lipin-1a, Leptin, and Adiponectin). BaP enhanced the INFG, especially MCP-1 and TNFα. Co-exposure to BaP and PCB153 showed a synergistic effect on TNFα and IL6 expression. Treatment with BaP and PCBs during only the maturation period up-regulated the INFG (IL6, TNFα, CXCL-10 & MCP-1). PCB118 alone also enhanced TNFα, CXCL-10, and PAI-1 expression. The change in MCP-1 protein expression was in agreement with that of the gene. Finally, the BaP-induced up-regulation of the xenobiotic responsive element (XRE)-controlled luciferase activity was impaired by PCB153 but not by PCB118; (3) Conclusion: BaP and PCBs down-regulate a part of ADGG and enhance INFG. The direct regulatory effect of PCBs on both ADGG and INFG is usually rather lower than that of BaP and synergistic or antagonistic cocktail effects are clearly observed.
Molecular Cloning and Characterization of Carbonic Anhydrase XII from Pufferfish (Takifugu rubripes)
Authors: Sumi Kanij , Kim Soo , Howlader Jewel , Lee Won , Choi Kap , Kim Hoy-Taek , Park Jong-In , Nou Ill-Sup , Kho Kang
Published: March 2018

In this study, an 1888-bp carbonic anhydrase XII (CA XII) sequence was cloned from the brain of the pufferfish, Takifugu rubripes. The cloned sequence contained a coding region of 1470-bp, which was predicted to translate into a protein of 490 amino acid residues. The predicted protein showed between 68–56% identity with the large yellow croaker (Larimichthys crocea), tilapia (Oreochromis niloticus), and Asian arowana (Scleropages formosus) CA XII proteins. It also exhibited 36% and 53% identity with human CA II and CA XII, respectively. The cloned sequence contained a 22 amino acid NH2-terminal signal sequence and three Asn-Xaa-Ser/Thr sequons, among which one was potentially glycosylated. Four cysteine residues were also identified (Cys-21, Cys-201, Cys-355, and Cys-358), two of which (Cys-21 and Cys-201) could potentially form a disulfide bond. A 22-amino acid COOH-terminal cytoplasmic tail containing a potential site for phosphorylation by protein kinase A was also found. The cloned sequence might be a transmembrane protein, as predicted from in silico and phylogenetic analyses. The active site analysis of the predicted protein showed that its active site residues were highly conserved with tilapia CA XII protein. Homology modeling of the pufferfish CA XII was done using the crystal structure of the extracellular domain of human carbonic anhydrase XII at 1.55 Å resolution as a template. Semi-quantitative reverse transcription (RT)-PCR, quantitative PCR (q-PCR), and in situ hybridization confirmed that pufferfish CA XII is highly expressed in the brain.
Using cAMP Sensors to Study Cardiac Nanodomains
Authors: Schleicher Katharina , Zaccolo Manuela
Published: March 2018

3′,5′-cyclic adenosine monophosphate (cAMP) signalling plays a major role in the cardiac myocyte response to extracellular stimulation by hormones and neurotransmitters. In recent years, evidence has accumulated demonstrating that the cAMP response to different extracellular agonists is not uniform: depending on the stimulus, cAMP signals of different amplitudes and kinetics are generated in different subcellular compartments, eliciting defined physiological effects. In this review, we focus on how real-time imaging using fluorescence resonance energy transfer (FRET)-based reporters has provided mechanistic insight into the compartmentalisation of the cAMP signalling pathway and allowed for the precise definition of the regulation and function of subcellular cAMP nanodomains.
Cross-Sectional Associations of Environmental Perception with Leisure-Time Physical Activity and Screen Time among Older Adults
Authors: Hsueh Ming-Chun , Lin Chien-Yu , Huang Pin-Hsuan , Park Jong-Hwan , Liao Yung
Published: March 2018

This study investigated associations of perceived environmental factors with leisure-time physical activity (LTPA) and screen time (ST) among older adults. A cross-sectional study was conducted by administering computer-assisted telephone interviews to 1028 older Taiwanese adults in November 2016. Data on personal factors, perceived environmental factors, LTPA, and ST were included. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to examine associations of environmental perception with LTPA and ST by using logistic regression analyses. The results showed that after adjusting for potential confounders, older adults who perceived their neighborhood with good access to shops (AS) and to public transportation (AT) were more likely to have sufficient LTPA (AS: OR = 1.64, 95% CI: 1.16–2.32; AT: OR = 1.43; 95% CI, 1.00–2.03) and less likely to have excessive ST (AS: OR = 0.70; 95% CI: 0.50–0.97; AT: OR = 0.64; 95% CI: 0.46–0.90). Different perceived environmental factors were also associated with LTPA and ST, respectively. This study highlights environment perception as a crucial factor for LTPA and ST. These findings suggest that policy makers and physical activity intervention designers should develop both common and individual environmental strategies to improve and increase awareness of the neighborhood environment to promote LTPA and reduce ST among older adults.
Small-Scale Renewable Energy Converters for Battery Charging
Authors: Ayob Mohd , Castellucci Valeria , Göteman Malin , Widén Joakim , Abrahamsson Johan , Engström Jens , Waters Rafael
Published: March 2018

This paper presents two wave energy concepts for small-scale electricity generation. In the presented case, these concepts are installed on the buoy of a heaving, point-absorbing wave energy converter (WEC) for large scale electricity production. In the studied WEC, developed by Uppsala University, small-scale electricity generation in the buoy is needed to power a tidal compensating system designed to increase the performance of the WEC in areas with high tides. The two considered and modeled concepts are an oscillating water column (OWC) and a heaving point absorber. The results indicate that the OWC is too small for the task and does not produce enough energy. On the other hand, the results show that a hybrid system composed of a small heaving point absorber combined with a solar energy system would be able to provide a requested minimum power of around 37.7 W on average year around. The WEC and solar panel complement each other, as the WEC produces enough energy by itself during wintertime (but not in the summer), while the solar panel produces enough energy in the summer (but not in the winter).
Reporting Key Features in Cold-Adapted Bacteria
Authors: Tribelli Paula , López Nancy
Published: March 2018

It is well known that cold environments are predominant over the Earth and there are a great number of reports analyzing bacterial adaptations to cold. Most of these works are focused on characteristics traditionally involved in cold adaptation, such as the structural adjustment of enzymes, maintenance of membrane fluidity, expression of cold shock proteins and presence of compatible solutes. Recent works based mainly on novel “omic” technologies have presented evidence of the presence of other important features to thrive in cold. In this work, we analyze cold-adapted bacteria, looking for strategies involving novel features, and/or activation of non-classical metabolisms for a cold lifestyle. Metabolic traits related to energy generation, compounds and mechanisms involved in stress resistance and cold adaptation, as well as characteristics of the cell envelope, are analyzed in heterotrophic cold-adapted bacteria. In addition, metagenomic, metatranscriptomic and metaproteomic data are used to detect key functions in bacterial communities inhabiting cold environments.
Energy Absorption Capacity in Natural Fiber Reinforcement Composites Structures
Authors: López-Alba Elías , Schmeer Sebastian , Díaz Francisco
Published: March 2018

The study of natural fiber reinforcement composite structures has focused the attention of the automobile industry due to the new regulation in relation to the recyclability and the reusability of the materials preserving and/or improving the mechanical characteristics. The influence of different parameters on the material behavior of natural fiber reinforced plastic structures has been investigated, showing the potential for transport application in energy absorbing structures. Two different woven fabrics (twill and hopsack) made of flax fibers as well as a non-woven mat made of a mixture of hemp and kenaf fibers were employed as reinforcing materials. These reinforcing textiles were impregnated with both HD-PE (high-density polyethylen) and PLA (polylactic acid) matrix, using a continuous compression molding press. The impregnated semi-finished laminates (so-called organic sheets) were thermoformed in a second step to half-tubes that were assembled through vibration-welding process to cylindric crash absorbers. The specimens were loaded by compression to determine the specific energy absorption capacity. Quasi-static test results were compared to dynamic test data obtained on a catapult arrangement. The differences on the specific energies absorption (SEA) as a function of different parameters, such as the wall thickness, the weave material type, the reinforced textiles, and the matrix used, depending on the velocity rate application were quantified. In the case of quasi-static analysis it is observed a 20% increment in the SEA value when wove Hopsack fabric reinforcement is employed. No velocity rate influence from the material was observed on the SEA evaluation at higher speeds used to perform the experiments. The influence of the weave configuration (Hopsack) seems to be more stable against buckling effects at low loading rates with 10% higher SEA values. An increase of SEA level of up to 72% for PLA matrix was observed when compared with HD-PE matrix.
Laser Induced Damage of Potassium Dihydrogen Phosphate (KDP) Optical Crystal Machined by Water Dissolution Ultra-Precision Polishing Method
Authors: Chen Yuchuan , Gao Hang , Wang Xu , Guo Dongming , Liu Ziyuan
Published: March 2018

Laser induced damage threshold (LIDT) is an important optical indicator for nonlinear Potassium Dihydrogen Phosphate (KDP) crystal used in high power laser systems. In this study, KDP optical crystals are initially machined with single point diamond turning (SPDT), followed by water dissolution ultra-precision polishing (WDUP) and then tested with 355 nm nanosecond pulsed-lasers. Power spectral density (PSD) analysis shows that WDUP process eliminates the laser-detrimental spatial frequencies band of micro-waviness on SPDT machined surface and consequently decreases its modulation effect on the laser beams. The laser test results show that LIDT of WDUP machined crystal improves and its stability has a significant increase by 72.1% compared with that of SPDT. Moreover, a subsequent ultrasonic assisted solvent cleaning process is suggested to have a positive effect on the laser performance of machined KDP crystal. Damage crater investigation indicates that the damage morphologies exhibit highly thermal explosion features of melted cores and brittle fractures of periphery material, which can be described with the classic thermal explosion model. The comparison result demonstrates that damage mechanisms for SPDT and WDUP machined crystal are the same and WDUP process reveals the real bulk laser resistance of KDP optical crystal by removing the micro-waviness and subsurface damage on SPDT machined surface. This improvement of WDUP method makes the LIDT more accurate and will be beneficial to the laser performance of KDP crystal.
Anti-Inflammatory Polyoxygenated Steroids from the Soft Coral Lobophytum michaelae
Authors: Huang Chiung-Yao , Tseng Wan-Ru , Ahmed Atallah , Chiang Pei-Lun , Tai Chi-Jen , Hwang Tsong-Long , Dai Chang-Feng , Sheu Jyh-Horng
Published: March 2018

Three new polyoxygenated steroids, michosterols A–C (1–3), and four known compounds (4–7) were isolated from the ethyl acetate (EtOAc) extract of the soft coral Lobophytum michaelae, collected off the coast of Taitung. The structures of the new compounds were elucidated on the basis of spectroscopic analyses and comparison of the nuclear magnetic resonance (NMR) data with related steroids. The cytotoxicity of compounds 1–3 against the proliferation of a limited panel of cancer cell lines was assayed. Compound 1 was found to display moderate cytotoxicity against adenocarcinomic human alveolar basal epithelial (A549) cancer cells. It also exhibited potent anti-inflammatory activity by suppressing superoxide anion generation and elastase release in N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (fMLP/CB)-stimulated human neutrophils. Furthermore, 3 could effectively inhibit elastase release, as well.
Failure Analysis of PHILOS Plate Construct Used for Pantalar Arthrodesis Paper I—Analysis of the Plate
Authors: Ina Jason , Vallentyne Madhurima , Hamandi Farah , Shugart Kathleen , Boin Michael , Laughlin Richard , Goswami Tarun
Published: March 2018

The failure of a proximal humerus internal locking system (PHILOS) used in a pantalar arthrodesis was investigated in this paper. PHILOS constructs are hybrids using locking and non-locking screws. Both the plate and the screws used in the fusion were obtained for analysis. However, only the plate failure analysis is reported in this paper. The implant had failed in several pieces. Optical and scanning electron microscopic analyses were performed to characterize the failure mode(s) and fracture surface. The chemical composition and mechanical properties of the plate were determined and compared to controlling specifications to manufacture the devices. We found that equivalent tensile strength exceeded at the locations of high stress, axial, and angular displacement and matched the specification at the regions of lower stress/displacement. Such a region-wise change in mechanical properties with in vivo utilization has not been reported in the literature. Evidence of inclusions was qualitatively determined for the stainless steel 316L plate failing the specifications. Pitting corrosion, scratches, discoloration and debris were present on the plate. Fracture surface showed (1) multi-site corrosion damage within the screw holes forming a 45° maximum shear force line for crack-linking, and (2) crack propagation perpendicular to the crack forming origin that may have formed due to the presence of inclusions. Fracture features such as beach marks and striations indicating that corrosion may have initiated the crack(s), which grew by fatigue over a period of time. In conclusion, the most likely mechanism of failure for the device was due to corrosion fatigue and lack of bony in-growth on the screws that may have caused loosening of the device causing deformity and pre-mature failure.
Identification of sucA, Encoding β-Fructofuranosidase, in Rhizopus microsporus
Authors: Orikasa Yoshitake , Oda Yuji , Ohwada Takuji
Published: March 2018

Rhizopus microsporus NBRC 32995 was found to hydrolyze fructooligosaccharides (FOS), as well as sucrose, almost completely into monosaccharides through the production of sufficient amounts of organic acids, indicating that the complete hydrolysis of FOS was caused by the secretion of β-fructofuranosidase from fungal cells. Thus, the sucA gene, encoding a β-fructofuranosidase, was amplified by degenerate PCR, and its complete nucleotide sequence was determined. The total length of the sucA gene was 1590 bp, and the SucA protein of R. microsporus NBRC 32995 belonged to clade VIa, which also contains Rhizopus delemar and is closely related to Saccharomycotina, a subdivision of the Ascomycota.
Eudesmane-Type Sesquiterpene Glycosides from Dictamnus dasycarpus Turcz.
Authors: Yang Shengcai , Li Zheng , Wang Jianli , Ruan Jingya , Zheng Chang , Huang Peijian , Han Lifeng , Zhang Yi , Wang Tao
Published: March 2018

Eudesmane-type sesquiterpenes have been reported to exhibit varieties of biological activities. During the process of investigating this kind of natural product from the root bark of Dictamnus dasycarpus Turcz., 13 eudesmane-type sesquiterpene glycosides including six new isolates, named as dictameudesmnosides A1 (1), A2 (2), B (3), C (4), D (5), and E (6), together with seven known ones (7–13), were obtained. Herein, their structures were determined by the analysis of physical data, spectroscopic analysis, and chemical methods. The existence of α-configuration glucose units in their structures (1–5, 8) is not very common in natural glycosidic components. Meanwhile, compounds 3–5, 7, and 9–13 displayed TG accumulation inhibitory effects on HepG2 cells.
In Vitro and In Vivo Short-Term Pulmonary Toxicity of Differently Sized Colloidal Amorphous SiO2
Authors: Wiemann Martin , Sauer Ursula , Vennemann Antje , Bäcker Sandra , Keller Johannes-Georg , Ma-Hock Lan , Wohlleben Wendel , Landsiedel Robert
Published: March 2018

In vitro prediction of inflammatory lung effects of well-dispersed nanomaterials is challenging. Here, the in vitro effects of four colloidal amorphous SiO2 nanomaterials that differed only by their primary particle size (9, 15, 30, and 55 nm) were analyzed using the rat NR8383 alveolar macrophage (AM) assay. Data were compared to effects of single doses of 15 nm and 55 nm SiO2 intratracheally instilled in rat lungs. In vitro, all four elicited the release of concentration-dependent lactate dehydrogenase, β-glucuronidase, and tumor necrosis factor alpha, and the two smaller materials also released H2O2. All effects were size-dependent. Since the colloidal SiO2 remained well-dispersed in serum-free in vitro conditions, effective particle concentrations reaching the cells were estimated using different models. Evaluating the effective concentration–based in vitro effects using the Decision-making framework for the grouping and testing of nanomaterials, all four nanomaterials were assigned as “active.” This assignment and the size dependency of effects were consistent with the outcomes of intratracheal instillation studies and available short-term rat inhalation data for 15 nm SiO2. The study confirms the applicability of the NR8383 AM assay to assessing colloidal SiO2 but underlines the need to estimate and consider the effective concentration of such well-dispersed test materials.
Use of Placebo in Supplementation Studies—Vitamin D Research Illustrates an Ethical Quandary
Authors: Frame Leigh , Fischer Jonathan , Geller Gail , Cheskin Lawrence
Published: March 2018

History has shown that without explicit and enforced guidelines, even well-intentioned researchers can fail to adequately examine the ethical pros and cons of study design choices. One area in which consensus does not yet exist is the use of placebo groups in vitamin supplementation studies. As a prime example, we focus on vitamin D research. We aim to provide an overview of the ethical issues in placebo-controlled studies and guide future discussion about the ethical use of placebo groups. Research in the field of vitamin D shows variation in how placebo groups are used. We outline four types of control groups in use: active-control, placebo-control with restrictions on supplementation, placebo-control without supplementation restrictions, and placebo-control with rescue repletion therapy. The first two types highlight discrete ethical issues: active-control trials limit the ability to detect a difference; placebo-control trials that restrict supplementation potentially place subjects at risk of undue harm. The final two, placebo-control without supplementation restrictions or with rescue repletion therapy, offer potential solutions to these ethical challenges. Building on this, guidelines should be established and enforced on the use of placebo in supplementation studies. Furthermore, the field of vitamin D research has the potential to set an example worthy of emulation.
A Comprehensive Review on the Medicinal Plants from the Genus Asphodelus
Authors: Malmir Maryam , Serrano Rita , Caniça Manuela , Silva-Lima Beatriz , Silva Olga
Published: March 2018

Plant-based systems continue to play an essential role in healthcare, and their use by different cultures has been extensively documented. Asphodelus L. (Asphodelaceae) is a genus of 18 species and of a total of 27 species, sub-species and varieties, distributed along the Mediterranean basin, and has been traditionally used for treating several diseases particularly associated with inflammatory and infectious skin disorders. The present study aimed to provide a general review of the available literature on ethnomedical, phytochemical, and biological data related to the genus Asphodelus as a potential source of new compounds with biological activity. Considering phytochemical studies, 1,8-dihydroxyanthracene derivatives, flavonoids, phenolic acids and triterpenoids were the main classes of compounds identified in roots, leaf and seeds which were correlated with their biological activities as anti-microbial, anti-fungal, anti-parasitic, cytotoxic, anti-inflammatory or antioxidant agents.
Reusable Xerogel Containing Quantum Dots with High Fluorescence Retention
Authors: Liang Xiang-Yong , Wang Lu , Chang Zhi-Yi , Ding Li-Sheng , Li Bang-Jing , Zhang Sheng
Published: March 2018

Although various analytical methods have been established based on quantum dots (QDs), most were conducted in solution, which is inadequate for storage/transportation and rapid analysis. Moreover, the potential environmental problems caused by abandoned QDs cannot be ignored. In this paper, a reusable xerogel containing CdTe with strong emission is established by introducing host–guest interactions between QDs and polymer matrix. This xerogel shows high QDs loading capacity without decrease or redshift in fluorescence (the maximum of loading is 50 wt % of the final xerogel), which benefits from the steric hindrance of β-cyclodextrin (βCD) molecules. Host–guest interactions immobilize QDs firmly, resulting in the excellent fluorescence retention of the xerogel. The good detecting performance and reusability mean this xerogel could be employed as a versatile analysis platform (for quantitative and qualitative analyses). In addition, the xerogel can be self-healed by the aid of water.
3D Printable Filaments Made of Biobased Polyethylene Biocomposites
Authors: Filgueira Daniel , Holmen Solveig , Melbø Johnny , Moldes Diego , Echtermeyer Andreas , Chinga-Carrasco Gary
Published: March 2018

Two different series of biobased polyethylene (BioPE) were used for the manufacturing of biocomposites, complemented with thermomechanical pulp (TMP) fibers. The intrinsic hydrophilic character of the TMP fibers was previously modified by grafting hydrophobic compounds (octyl gallate and lauryl gallate) by means of an enzymatic-assisted treatment. BioPE with low melt flow index (MFI) yielded filaments with low void fraction and relatively low thickness variation. The water absorption of the biocomposites was remarkably improved when the enzymatically-hydrophobized TMP fibers were used. Importantly, the 3D printing of BioPE was improved by adding 10% and 20% TMP fibers to the composition. Thus, 3D printable biocomposites with low water uptake can be manufactured by using fully biobased materials and environmentally-friendly processes.
An Underwater Image Enhancement Algorithm for Environment Recognition and Robot Navigation
Authors: Xie Kun , Pan Wei , Xu Suxia
Published: March 2018

There are many tasks that require clear and easily recognizable images in the field of underwater robotics and marine science, such as underwater target detection and identification of robot navigation and obstacle avoidance. However, water turbidity makes the underwater image quality too low to recognize. This paper proposes the use of the dark channel prior model for underwater environment recognition, in which underwater reflection models are used to obtain enhanced images. The proposed approach achieves very good performance and multi-scene robustness by combining the dark channel prior model with the underwater diffuse model. The experimental results are given to show the effectiveness of the dark channel prior model in underwater scenarios.
A Decadal Historical Satellite Data and Rainfall Trend Analysis (2001–2016) for Flood Hazard Mapping in Sri Lanka
Authors: Alahacoon Niranga , Matheswaran Karthikeyan , Pani Peejush , Amarnath Giriraj
Published: March 2018

Critical information on a flood-affected area is needed in a short time frame to initiate rapid response operations and develop long-term flood management strategies. This study combined rainfall trend analysis using Asian Precipitation—Highly Resolved Observational Data Integration towards Evaluation of Water Resources (APHRODITE) gridded rainfall data with flood maps derived from Synthetic Aperture Radar (SAR) and multispectral satellite to arrive at holistic spatio-temporal patterns of floods in Sri Lanka. Advanced Land Observing Satellite Phased Array type L-band Synthetic Aperture Radar (ALOS PALSAR) data were used to map flood extents for emergency relief operations while eight-day Moderate Resolution Imaging Spectroradiometer (MODIS) surface reflectance data for the time period from 2001 to 2016 were used to map long term flood-affected areas. The inundation maps produced for rapid response were published within three hours upon the availability of satellite imagery in web platforms, with the aim of supporting a wide range of stakeholders in emergency response and flood relief operations. The aggregated time series of flood extents mapped using MODIS data were used to develop a flood occurrence map (2001–2016) for Sri Lanka. Flood hotpots identified using both optical and synthetic aperture average of 325 km2 for the years 2006–2015 and exceptional flooding in 2016 with inundation extent of approximately 1400 km2. The time series rainfall data explains increasing trend in the extreme rainfall indices with similar observation derived from satellite imagery. The results demonstrate the feasibility of using multi-sensor flood mapping approaches, which will aid Disaster Management Center (DMC) and other multi-lateral agencies involved in managing rapid response operations and preparing mitigation measures.
Long Short-Term Memory Neural Networks for Online Disturbance Detection in Satellite Image Time Series
Authors: Kong Yun-Long , Huang Qingqing , Wang Chengyi , Chen Jingbo , Chen Jiansheng , He Dongxu
Published: March 2018

A satellite image time series (SITS) contains a significant amount of temporal information. By analysing this type of data, the pattern of the changes in the object of concern can be explored. The natural change in the Earth’s surface is relatively slow and exhibits a pronounced pattern. Some natural events (for example, fires, floods, plant diseases, and insect pests) and human activities (for example, deforestation and urbanisation) will disturb this pattern and cause a relatively profound change on the Earth’s surface. These events are usually referred to as disturbances. However, disturbances in ecosystems are not easy to detect from SITS data, because SITS contain combined information on disturbances, phenological variations and noise in remote sensing data. In this paper, a novel framework is proposed for online disturbance detection from SITS. The framework is based on long short-term memory (LSTM) networks. First, LSTM networks are trained by historical SITS. The trained LSTM networks are then used to predict new time series data. Last, the predicted data are compared with real data, and the noticeable deviations reveal disturbances. Experimental results using 16-day compositions of the moderate resolution imaging spectroradiometer (MOD13Q1) illustrate the effectiveness and stability of the proposed approach for online disturbance detection.