The State of the Neuronal-Astrocytic Apparatus of the Hippocampus on the Background of Long-Term Administration of Isoniazid and Rifampicin under Conditions of Correction of the Gut Microflora
Authors:   Dnipro State Medical University, 9, Vernadsky St., Dnipro 49044, Ukraine, Kharchenko Yu. V. , Myakushko V. A. ,   Dnipro Medical Institute Traditional and Non-traditonal Medicine. 17, Sevastopolskaya St., Dnipro 49001, Ukraine, Trushenko O. S. ,   Dnipro Medical Institute Traditional and Non-traditonal Medicine. 17, Sevastopolskaya St., Dnipro 49001, Ukraine, Yevtushenko T. V. ,   Dnipro Medical Institute Traditional and Non-traditonal Medicine. 17, Sevastopolskaya St., Dnipro 49001, Ukraine, Yevtushenko N. V. ,   Dnipro Medical Institute Traditional and Non-traditonal Medicine. 17, Sevastopolskaya St., Dnipro 49001, Ukraine, Derkach A. K. ,   Dnipro Medical Institute Traditional and Non-traditonal Medicine. 17, Sevastopolskaya St., Dnipro 49001, Ukraine, Kravchenko M. K. ,   Dnipro Medical Institute Traditional and Non-traditonal Medicine. 17, Sevastopolskaya St., Dnipro 49001, Ukraine
Published: December 2021

The purpose of the study was to determine the morphometric and ultrastructural features of neurons and glial cells, as well as the levels of glial fibrillar acid protein in the hippocampus of rats under long-term administration of isoniazid and rifampicin during experimental therapy with a combination of pro/prebiotics. Materials and methods. The studies were conducted on three groups of white Wistar male rats with drug-induced hepatitis, reproduced by intragastric administration of isoniazid and rifampicin for 28 days. In the last 14 days of the intragastric experiment, rats of the research group were administered probiotic and prebiotic. The content of cytosolic glial fibrillar acid protein in the hippocampus was determined by competitive enzyme-linked immunosorbent assay. Semi-thin sections of CA1 sections of the rat hippocampus were analyzed using the Image J. analysis program. The ultrastructural characteristics were studied using a PEM-100-01 transmission electron microscope (Selmi, Ukraine). Results and discussion. Long-term administration of isoniazid and rifampicin was associated with changes in the content of the cytosolic fraction of glial fibrillar acid protein obtained from the hippocampus. These changes were characterized by a tendency to increase the level of this protein by 17.8% (p=0.2) compared with intact animals. In contrast to the group of animals reproduced in the experiment drug-induced liver injury, in the experimental group, the level of glial fibrillar acid protein was significantly lower by 25.0% compared to the drug-induced liver injury group (p <0.05). Morphological analysis revealed a decrease in neuronal density in rats with drug-induced liver injury compared with intact control. At the same time, the specific number of degeneratively altered neurons in comparison with intact control in the group of drug-induced liver injury animals increased by 8.57 times. The number of degeneratively altered neurons in the group receiving yogurt / lactulose was 55.87 ± 4.23%, which was significantly higher than intact control rats, but was 19.4% (p <0.05) lower levels in rats with MIUP. Electron microscopic examination of rat hippocampal neuroglia was based on the study of changes in the astrocytic and oligodendroglial components. Changes in rat hippocampal neuroglia under experimental conditions were mainly related to the astrocytic link. Astrocytes were characterized by significantly lower cytoplasmic edema and adaptive-compensatory changes in the cell. Conclusion. Course administration of pro/prebiotics reduces the severity of manifestations of neurodegeneration, improves the state of astroglia in the hippocampus, and reduces the cytoplasmic levels of glial acidic fibrillar protein in animals with drug-induced liver injury