bluuebunny/crossref_metadata_2025 huggingface dataset:
Plant tissue culture environment as a switch-key of (epi)genetic changes
Authors: Bednarek Piotr Tomasz , Orłowska Renata
Published: October 2019

The in vitro tissue cultures are, beyond all difficulties, an essential tool in basic research as well as in commercial applications. Numerous works devoted to plant tissue cultures proved how important this part of the plant science is. Despite half a century of research on the issue of obtaining plants in in vitro cultures, many aspects remain unknown. The path associated with the reprogramming of explants in the fully functioning regenerants includes a series of processes that may result in the appearance of morphological, physiological, biochemical or, finally, genetic and epigenetic changes. All these changes occurring at the tissue culture stage and appearing in regenerants as tissue culture-induced variation and then inherited by generative progeny as somaclonal variation may be the result of oxidative stress, which works at the step of explant preparation, and in tissue culture as a result of nutrient components and environmental factors. In this review, we describe the current status of understanding the genetic and epigenetic changes that occur during tissue culture.
Diversity of root-associated fungi of Rhododendron simsii in subtropical forests: fungal communities with high resistance to anthropogenic disturbances
Authors: Zhang Yanhua , Tang Fangping , Ni Jian , Dong Lijia , Sun Lifu
Published: October 2019

Species of the Ericaceae or heath family are widely distribute in continental ecosystems and their special ericoid mycorrhizas (ERM) are considered beneficial to their survival and persistence in variable habitats. Currently, increasing anthropogenic disturbances and improper forest management are affecting subtropical forests of China where these native species located. These activities not only affect plant communities above-ground, but also impose pressures on microbial communities below- ground. In this study, root-associated fungal communities of Rhododendron simsii in four forest types under different anthropogenic disturbances were identified using an Illumina Miseq platform, i.e., old growth forests, secondary forests with one cutting (SEC I), secondary forests with two cuttings (SEC II), and Chinese-fir plantations (PLF). Intra- and inter-annual variations were analyzed by comparing samples taken in different seasons and years. The results show that: (1) over 1000 OTUs were found in hair roots with most from the division Ascomycota and Basidiomycota belonging to different functional groups; (2) while there were a few indicator OTUs specific to different forest types, seasons and years, the proportion of shared taxa was quite large, accounting for 44.9–79.4% of the total OTUs; (3) significantly positive correlations were found between disturbance sensitivity and temporal variations in common fungal orders, and both in major fungal orders were significantly different among fungal functional groups in which putative and possible ERM fungi were highly resistant to disturbances and low temporal variations. The high disturbance resistance and temporal persistence of putative ERM fungi may be essential for the successful adaptation of R. simsii in disturbed subtropical forests of China.
Dimensions of Religious Harmony as Constitutional Practice: Beyond State Control
Authors: Neo Jaclyn L.
Published: October 2019

Religious harmony is an idea more commonly invoked in Asian countries, many of which are closely associated with non-individualistic or non-liberal approaches to law, ethics, and politics, than in Europe. As a constitutional norm, religious harmony not only directs state action involving the management of religious diversity but also has the potential to legitimate state action. As a result, harmony, including its subspecies of religious harmony, could be and has been criticized for imposing and legitimating an ideology of control over society, particularly over marginalized groups. While this is the case, I argue in this Article that religious harmony can mean many things and can be used in a myriad of ways that go beyond simply as a tool for state control. Religious harmony is not only a legal/constitutional principle, but has also become internalized as a social norm. Its regulating function extends to inter group relations and further grounds group demands on the state, thus imposing state obligations. To draw out the multiple and complex dimensions of religious harmony as a constitutional principle and social norm, I use Singapore, a self-avowed non-liberal communitarian state, as the primary case study in this Article.
Russia – Measures Concerning Traffic in Transit (WTO)
Authors: Hartmann Stephanie
Published: October 2019

On April 5, 2019, a World Trade Organization (WTO) panel (Panel) issued its decision in the dispute Russia – Measures Concerning Traffic in Transit. This decision is notable because it is the first instance in which a WTO panel has been called on to interpret the national security provision of the General Agreement on Tariffs and Trade (GATT 1994), Article XXI.
Tecnología cerámica en la Puna meridional argentina (Antofagasta de la Sierra, Catamarca): Cambios y continuidades en los modos de hacer a lo largo del tiempo (ca. 2000-500 aP)
Authors: Gasparotti Leticia I.
Published: October 2019

Este trabajo busca profundizar el conocimiento sobre el rol de los sectores intermedios de las quebradas en el entramado social de la microrregión de Antofagasta de la Sierra (Puna meridional argentina) a lo largo del tiempo. A través del estudio de la tecnología cerámica desde una perspectiva diacrónica (ca. 2000-500 aP), haciendo énfasis en los estudios petrográficos de pastas, se investigan los cambios y continuidades en la producción alfarera de la microrregión. Para ello, se presentan los resultados de la investigación realizada sobre el material cerámico proveniente de los sitios de Las Escondidas y Corral Alto, en la quebrada del Río Miriguaca, y Cueva Salamanca 1, en la quebrada del Río Las Pitas. A partir de los análisis realizados, se pudo concluir que existieron cambios en los modos de hacer cerámica a lo largo del tiempo en la microrregión. Se observó que durante el Formativo (ca. 2000 aP) existió un modo de hacer compartido en un área amplia, mientras que durante el periodo Tardío (ca. 800 aP) se dio una fragmentación en las prácticas de producción cerámica en la microrregión. Estos cambios responden a profundas trasformaciones ocurridas en las sociedades puneñas que se comenzaron a gestar tiempo antes del comienzo del período Tardío en un proceso paulatino, complejo y heterogéneo.
It's a (coarsened exact) match! Non-parametric imputation of European abstainers' vote
Authors: Bol Damien , Giani Marco
Published: October 2019

There is a long tradition of imputation studies looking at how abstainers would vote if they had to. This is crucial for democracies because when abstainers and voters have different preferences, the electoral outcome ceases to reflect the will of the people. In this paper, we apply a non-parametric method to revisit old evidence. We impute the vote of abstainers in 15 European countries using Coarsened Exact Matching (CEM). While traditional imputation methods rely on the choice of voters that are on average like abstainers, and simulate full turnout, CEM only imputes the vote of the abstainers that are similar to voters, and allows to simulate an electoral outcome under varying levels of turnout, including levels that credibly simulate compulsory voting. We find that higher turnout would benefit social democratic parties while imposing substantial losses to extreme left and green parties.
Crayfish bio-gastroliths from eastern Australia and the middle Cretaceous distribution of Parastacidae
Authors: Bell Phil R. , Bicknell Russell D. C. , Smith Elizabeth T.
Published: October 2019

Fossil crayfish are typically rare, worldwide. In Australia, the strictly Southern Hemisphere clade Parastacidae, while ubiquitous in modern freshwater systems, is known only from sparse fossil occurrences from the Aptian–Albian of Victoria. We expand this record to the Cenomanian of northern New South Wales, where opalized bio-gastroliths (temporary calcium storage bodies found in the foregut of pre-moult crayfish) form a significant proportion of the fauna of the Griman Creek Formation. Crayfish bio-gastroliths are exceedingly rare in the fossil record but here form a remarkable supplementary record for crayfish, whose body and trace fossils are otherwise unknown from the Griman Creek Formation. The new specimens indicate that parastacid crayfish were widespread in eastern Australia by middle Cretaceous time, occupying a variety of freshwater ecosystems from the Australian–Antarctic rift valley in the south, to the near-coastal floodplains surrounding the epeiric Eromanga Sea further to the north.
The Modernity of Caste and the Market Economy
Authors: MOSSE DAVID
Published: October 2019

What place does the caste system have in modern India with its globally integrating market economy? The most influential anthropological approaches to caste have tended to emphasize caste as India's traditional religious and ritual order, or (treating such order as a product of the colonial encounter) as shaped politically, especially today by the dynamics of caste-based electoral politics. Less attention has been paid to caste effects in the economy. This article argues that the scholarly framing of caste mirrors a public-policy ‘enclosure’ of caste in the non-modern realm of religion and ‘caste politics’, while aligning modernity to the caste-erasing market economy. Village-level fieldwork in South India finds a parallel public narrative of caste either as ritual rank eroded by market relations or as identity politics deflected from everyday economic life. But, locally and nationally, the effects of caste are found to be pervasive in labour markets and the business economy. In the age of the market, caste is a resource, sometimes in the form of a network, its opportunity-hoarding advantages discriminating against others. Dalits are not discriminated by caste as a set of relations separate from economy, but by the very economic and market processes through which they often seek liberation. The caste processes, enclosures, and evasions in post-liberalization India suggest the need to rethink the modernity of caste beyond orientalist and post-colonial frameworks, and consider the presuppositions that shape understanding of an institution, the nature and experience of which are determined by the inequalities and subject positions it produces.
Social sin, social redemption
Authors: POGIN KATHRYN
Published: October 2019

Eleonore Stump's Atonement marks a significant advance in atonement theory, especially in its nuanced approach to ethical and relational complexities, but tends to treat sin as social only insofar as one individual's sin can harm or shame another. I argue that that social sin requires social redemption and that exemplarism would provide a solution. Christ's pursuit of love and justice, in the midst of oppression, temptation, and struggle, offers a distinctive model of virtue, towards collective restoration of the world. While we cannot redeem ourselves, in calling us to effect justice and union with one another, God may also call us closer to Godself.
Private Benefits? External Benefits? Outcomes of Private Schooling in 21stCentury Britain
Authors: GREEN FRANCIS , ANDERS JAKE , HENDERSON MORAG , HENSEKE GOLO
Published: October 2019

Policy discourse surrounding Britain’s unusually well-resourced private schools surrounds their charitable status and their relationship with low social mobility, but informative evidence is scarce. We present estimates of the extent to which private and external benefits at age 25 are associated with attendance at private school in England in the 21st century. We find a weekly wage premium of 17 percent, and a 12 percentage point lower chance of downward social mobility. By contrast, private schooling is not significantly associated with participation in local voluntary groups, unpaid voluntary work, or charitable giving and fundraising; this finding casts doubt on claims that private schools deliver ‘public benefit’ in this way.
Facilitators and barriers to autonomy: a systematic literature review for older adults with physical impairments, living in residential care facilities
Authors: van Loon Jolande , Luijkx Katrien , Janssen Meriam , de Rooij Ietje , Janssen Bienke
Published: October 2019

Autonomy is important in every stage of life. However, little is known about how autonomy is enhanced for older adults living in residential care facilities (RCFs). This leads to the research question: which facilitators and barriers to autonomy of older adults with physical impairments due to ageing and chronic health conditions living in RCFs are known? The results will be organised according to the framework of person-centred practice, because this is related to autonomy enhancement. To answer the research question, a systematic literature search and review was performed in the electronic databases CINAHL, PsycINFO, PubMed, Social Services Abstracts and Sociological Abstracts. Inclusion and exclusion criteria were derived from the research question. Selected articles were analysed and assessed on quality using the Mixed Methods Appraisal Tool. Facilitators and barriers for autonomy were found and arranged in four themes: characteristics of residents, prerequisites of professional care-givers, care processes between resident and care-giver, and environment of care. The established facilitators and barriers are relational and dynamic. For a better understanding of the facilitators and barriers to autonomy for older adults with physical impairments living in RCFs, a description is based on the 35 included articles. Autonomy is a capacity to influence the environment and make decisions irrespective of having executional autonomy, to live the kind of life someone desires to live in the face of diminishing social, physical and/or cognitive resources and dependency, and it develops in relationships. The results provide an actual overview and lead to a better understanding of barriers and facilitators for the autonomy of older adults with physical impairments in RCFs. For both residents and care-givers, results offer possibilities to sustain and reinforce autonomy. Scientifically, the study creates new knowledge on factors that influence autonomy, which can be used to enhance autonomy.
Going Against Whose Grain? Archaeological Theory and Southeast Asia's Premodern States
Authors: Stark Miriam T.
Published: October 2019

Southeast Asia is a paradox to Western scholars. Few are familiar with its history, yet Southeast Asia has been a veritable intellectual resource extraction zone for twentieth- and twenty-first-century social thought: imagined communities, galactic polities, agricultural involution and the moral economy of peasants all emanate from work done in Southeast Asia. The region's archaeological record is equally paradoxical: late Pleistocene ‘Hobbit’ hominins disrupt models of human origins, the world's largest Buddhist monument of Borobudur now sits in a wholly Muslim land mass in central Java, and the world's largest premodern city of Angkor is located in Cambodia, a country that remains resolutely rural. So we should not be surprised that Scott'sAgainst the Grain: A deep history of the earliest statesdraws from a career in Southeast Asian studies to study human history (the entire Anthropocene). This essay concentrates on how Scott believes early Mesopotamian states became legible.
A situs solitus transposition of great arteries with obstructed sub-diaphragmatic totally anomalous pulmonary venous connection: a rare case treated with anatomical repair
Authors: Meliota Giovanni , Scalzo Gabriele , Vairo Ugo
Published: October 2019

Transposition of the great arteries combined with totally anomalous pulmonary venous connection is extremely rare outside of heterotaxy syndrome. Most reported cases have been treated by a modified atrial switch operation. We report the successful treatment of a neonate with this rare association, repaired by arterial switch operation and connection of the pulmonary venous return to the left atrium.
Childhood bilingualism and Specific Language Impairment: A study of the CP-domain in German SLI
Authors: Ruberg Tobias , Rothweiler Monika , Veríssimo João , Clahsen Harald
Published: October 2019

This study addresses the question of whether and how growing up with more than one language shapes a child's language impairment. Our focus is on Specific Language Impairment (SLI) in bilingual (Turkish–German) children. We specifically investigated a range of phenomena related to the so-called CP (Complementizer Phrase) in German, the hierarchically highest layer of syntactic clause structure, which has been argued to be particularly affected in children with SLI. Spontaneous speech data were examined from bilingual children with SLI in comparison to two comparison groups: (i) typically-developing bilingual children, (ii) monolingual children with SLI. We found that despite persistent difficulty with subject-verb agreement, the two groups of children with SLI did not show any impairment of the CP-domain. We conclude that while subject-verb agreement is a suitable linguistic marker of SLI in German-speaking children, for both monolingual and bilingual ones, ‘vulnerability of the CP-domain’ is not.
Preventing unintentional home injuries among children: exploring the perceptions of Iranian health professionals
Authors: Barat Atena , Watson Michael Craig , Mulvaney Caroline A.
Published: October 2019

Background:

Health professionals are key stakeholders who potentially have important roles in preventing unintentional child home injuries. This study aimed to identify facilitators and barriers to the prevention of unintentional child home injuries perceived by health professionals.

Design:

A generic qualitative study involving semi-structured interviews.

Setting:

The capital city of Iran.

Method:

Data for this study were collected through 28 in-depth interviews with health professionals. Purposive sampling was conducted from three areas of Tehran based on their socio-economic development.

Findings:

Thematic data analysis yielded nine overarching themes: prioritising child home injury, knowledge, the nature of injury and injury prevention, child-related factors, parent-related factors, living environment, cultural issues, resources and management.

Conclusions:

Health professionals can potentially be supportive to meet families’ needs. However, further support and resources will be required if they are to fully develop their potential in preventing injuries in the home. The lack of a national action plan was a significant constraint for health professionals.
Comparing the star formation history of three nearby disk galaxies
Authors: Chang Ruixiang , Kang Xiaoyu , Zhang Fenghui
Published: October 2019

Understanding the effect of environment on galaxy formation and evolution is one of the hot topics in extragalactic astronomy. Here we constructed a chemical evolution model of disk galaxies. By comparing the model predictions with the observed profiles, we investigated the star formation history of M33, NGC 300 and NGC 2403. We found that M33 has much longer infall timescale than NGC 300 and NGC 2403, and the star formation process of M33 is still active at later phase. Our results suggested that the cold gas supply of M33 is sufficient in the present-day, which may originate from the HI bridge between M33 and M31. In other words, we argue that the local environment plays an important role on the star formation history of a galaxy, at least for M33.
What are the implications of stellar chemical abundances in dwarf galaxies?
Authors: Suda Takuma , Aoki Wako , Katsuta Yutaka , Yamada Shimako , Matsuno Tadafumi , Iwata Saku , Takei Yuki , Fujimoto Masayuki Y.
Published: October 2019

We analyse the chemical abundances of stars in the local group dwarf galaxies using the SAGA database. The inspection of the relationship between Eu and Ba abundances confirms an anomalously Ba-rich population in Fornax, which indicates a pre-enrichment of interstellar gas with r-process elements.
Dwarfs in the entourage of the Local Volume groups: flow tracers and cosmological probes
Authors: Kashibadze Olga , Karachentsev Igor , Karachentseva Valentina
Published: October 2019

We consider a sample of dwarf galaxies with accurate distances and velocities around 14 massive groups in the Local Volume. We combine all the data into a single synthetic group, and then determine its radius of the zero-velocity surface, separating it against the global cosmic expansion. Our estimation is derived from fitting the the spherical infall model (including effects of the cosmological constant) to the observational data.

We found the optimal value of the radius to be 0.93 ± 0.02 Mpc. Assuming the Planck model parameters, it corresponds to the total mass of the synthetic group (1.6 ± 0.2) × 1012M⊙. Thus, we obtain the paradoxical result that the total mass of the synthetic group estimated on the scale of 3–4 its virial radius is only 60% of the virial mass estimate. Anyway, we conclude that wide outskirts of the nearby groups do not contain a large amount of hidden mass outside their virial radii.
Star formation history and dynamical evolution of the solar neighbourhood
Authors: Just Andreas , Moetazedian Reza , Polyachenko Evgeny
Published: October 2019

Based on cosmological re-simulations we have shown that the impact of satellite galaxies has a minor effect on the thin disc heating. In contrast satellite galaxies can generate long-lived warps of the outer disc and they can advance or delay bar formation significantly.
Dwarf Galaxies as Cosmological Probes
Authors: Navarro Julio F.
Published: October 2019

The Lambda Cold Dark Matter (LCDM) paradigm makes specific predictions for the abundance, structure, substructure and clustering of dark matter halos, the sites of galaxy formation. These predictions can be directly tested, in the low-mass halo regime, by dark matter-dominated dwarf galaxies. A number of potential challenges to LCDM have been identified when confronting the expected properties of dwarfs with observation. I review our understanding of a few of these issues, including the “missing satellites” and the “too-big-to-fail” problems, and argue that neither poses an insurmountable challenge to LCDM. Solving these problems requires that most dwarf galaxies inhabit halos of similar mass, and that there is a relatively sharp minimum halo mass threshold to form luminous galaxies. These predictions are eminently falsifiable. In particular, LCDM predicts a large number of “dark” low-mass halos, some of which should have retained enough primordial gas to be detectable in deep 21 cm or Hα surveys. Detecting this predicted population of “mini-halos” would be a major discovery and a resounding success for LCDM on small scales.
Ultra-Compact High Velocity Clouds as Minihalos and Dwarf Galaxies
Authors: Faerman Yakov , Sternberg Amiel , McKee Christopher F.
Published: October 2019

We present dark-matter minihalo models for the Ultra-Compact High Velocity HI Clouds (UCHVCs) recently discovered in the 21 cm ALFALFA survey. We assume gravitational confinement of ~104 K HI gas by flat-cored dark-matter subhalos within the Local Group. For the UCHVCs we calculate the photoionization-limited hydrostatic gas profiles for any distance-dependent total observed HI mass and predict the associated (projected) HI half-mass radii. The observed 21 cm fluxes and half-mass angular radii then constrain the source distances or DM halo parameters. As a consistency check we model the gas-rich dwarf galaxy Leo T, for which the distance is known (420 kpc) and there is a well-resolved HI column density profile. We derive an upper limit for the pressure of any enveloping hot IGM gas at the distance of Leo T. Our analysis supports the scenario that some of the UCHVCs may constitute a population of 21-cm-selected but optically-faint dwarf galaxies in the Local Volume.
An Analysis of M31 and its Satellite Galaxies Using RR Lyrae Variables
Authors: Tanakul Nahathai , Sarajedini Ata
Published: October 2019

RR Lyrae stars are powerful tools to study their host populations. Information such as distance, metallicity, reddening, and age can be obtained from their pulsation properties. Dwarf spheroidal (dSph) galaxies are the most common type of galaxy in the Local Group. They are found around massive hosts such as the Milky Way (MW) and M31 and are suggested to be the present-day counterparts to systems from which spheroids and stellar halos of larger galaxies were assembled. By comparing RR Lyraes in these dSphs with their host galaxies, we hope to understand more about the formation of these galaxies. In order to achieve this goal, we have analyzed six fields in M31 using archival imaging from the Hubble Space Telescope. Published data for M31, M33, and several M31 dSphs are also included. The results are then compared with those in the MW to better constrain the early history of these systems.
Simulations at the Dwarf Scale: From Violent Dwarfs at Cosmic Dawn and Cosmic Noon to Quiet Discs today
Authors: Ceverino Daniel
Published: October 2019

Dwarf galaxies with stellar masses around 109M⊙ can be explored at high and low redshifts and they give a glimpse of the different conditions of galaxy formation at different epochs. Using a large sample of about 300 zoom-in cosmological hydrodynamical simulations of galaxy formation I will briefly describe the formation of dwarfs at this mass scale at 3 different epochs: cosmic dawn (Ceverino, Klessen, Glover 2018), cosmic noon (Ceverino, Primack, Dekel 2015), and today (Ceverino et al. 2017). I will describe the FirstLight simulations of first galaxies at redshifts 5-15. These first dwarfs have extremely high star formation efficiencies due to high gas fractions and high gas accretion rates. These simulations will make predictions that will be tested for the first time with the James Webb Space Telescope (JWST). At cosmic noon, z = 2, galaxy formation is still a very violent and dynamic process. The VELA simulations have generated a set of dispersion-dominated dwarfs that show an elongated morphology due to their prolate dark-matter halos. Between z = 1 and 0, the AGORA simulation shows the formation of a low-mass disc due to slow gas accretion. The disc agrees with many local scaling relations, such as the stellar-mass-halo-mass and the baryonic Tully-Fisher relation.
Dwarf galaxies as hosts of stellar explosions: gas kinematics and abundances in 3D
Authors: Thöne C. C. , Izzo L. , Flores H. , Vergani S. , Christensen L.
Published: October 2019

The hosts of long Gamma-ray bursts (GRBs) are places of intense star-formation, which, at low redshift, are primarily low-mass dwarf starburst galaxies. Spatially resolved studies of these galaxies are still sparse, even more so at high spectral resolution where we can probe gas kinematics, in- and outflows and differences in abundance between different components. Here we present the first high resolution IFU sample of six low redshift GRB hosts, all dwarf starbursts. All galaxies in our sample show evidence for excess emission or broad emission components, with velocities of 100-200 km s−1. For GRB 030329, outflowing gas had also been observed in absorption in spectra of the GRB afterglow. The high velocity emission is usually blue shifted, connected to the brightest star-forming regions and more metal rich than the narrow component associated with the emission of the general host ISM. This gives strong indications that the excess emission/broad component is indeed associated to a starburst wind as observed in many field star-burst galaxies and a sign for the intense ongoing star-formation in those galaxies.
Red Supergiants as Chemical Abundance Probes: The Local Group dwarf NGC6822
Authors: Patrick Lee R. , Evans Chris J. , Davies Ben , Kudritzki Rolf-Peter , Bergemann Maria , Ferguson Annette N. M.
Published: October 2019

Red Supergiant Stars (RSGs) are important probes of stellar and chemical evolution in star-forming environments. They represent the brightest near-IR stellar components of external galaxies and probe the most recent stellar population to provide robust, independent abundance estimates. The Local Group dwarf irregular galaxy, NGC6822, is a reasonably isolated galaxy with an interesting structure and turbulent history. Using RSGs as chemical abundance probes, we estimate metallicities in the central region of NGC6822, finding a suggestion of a metallicity gradient (in broad agreement with nebular tracers), however, this requires further study for confirmation. With intermediate resolution Multi-object spectroscopy (from e.g. KMOS, EMIR, MOSFIRE) combined with state-of-the-art stellar model atmospheres, we demonstrate how RSGs can be used to estimate stellar abundances in external galaxies. In this context, we compare stellar and nebular abundance tracers in NGC 6822 and by combining stellar and nebular tracers we estimate an abundance gradient of −0.18 ± 0.05 dex/kpc.
Influence of the Void Environment on Chemical Abundances in Dwarf Galaxies and Implications for Connecting Star Formation and Halo Mass
Authors: Douglass Kelly A. , Vogeley Michael S. , Cen Renyue
Published: October 2019

We study how the void environment affects the chemical evolution of galaxies by comparing the metallicity of dwarf galaxies in voids with dwarf galaxies in denser regions. Using spectroscopic observations from SDSS DR7, we estimate oxygen and nitrogen abundances of 889 void dwarf galaxies and 672 dwarf galaxies in denser regions. A substitute for the [OII] λ3727 doublet is developed, permitting oxygen abundance estimates of SDSS dwarf galaxies at all redshifts with the direct method. We find that void dwarf galaxies have about the same oxygen abundances and slightly lower N/O ratios than dwarf galaxies in denser environments. The lower N/O ratios seen in void dwarf galaxies may indicate both delayed star formation and a dependence of cosmic downsizing on the large-scale environment. Similar oxygen abundances in the two dwarf galaxy populations might be evidence of larger ratios of dark matter halo mass to stellar mass in voids.
Morphology of stellar populations in the Magellanic Clouds using the VMC survey
Authors: Youssoufi Dalal El , Cioni Maria-Rosa L. , Bell Cameron P. M. , Rubele Stefano , Niederhofer Florian , Matijevic Gal
Published: October 2019

The Magellanic Clouds are nearby dwarf irregular galaxies that represent a unique laboratory for studying galaxy interactions. Their morphology and dynamics have been heavily influenced by their mutual interactions as well as with their interaction(s) with the Milky Way. We use the VISTA near-infrared YJKs survey of the Magellanic Clouds system (VMC) in combination with stellar partial models of the Large Magellanic Cloud (LMC), the Small Magellanic Cloud (SMC) and the Milky Way to investigate the spatial distribution of stellar populations of different ages across the Magellanic Clouds. In this contribution, we present the results of these studies that allow us to trace substructures possibly related to the interaction history of the Magellanic Clouds.
Star formation and environment: a step in understanding the formation and evolution of local dwarf galaxies
Authors: Sacchi Elena , Cignoni Michele , Aloisi Alessandra , Tosi Monica
Published: October 2019

We present here the results obtained from studying the resolved stellar populations of two dwarf irregular galaxies in the nearby Universe. These galaxies, DDO 68 and NGC 4449, were studied within the Legacy ExtraGalactic UV Survey, an HST program aimed to uncover the many ways in which the star formation (SF) process occurs at different scales. Thanks to the deep photometry obtained in different bands (from λ2704 Å to λ8057 Å), we were able to connect the location and timescales of the star forming regions within the galaxies to merging and interaction with gas clouds and satellites, a crucial aspect of galaxy evolution, even in such small systems. From the color-magnitude diagrams of the analyzed galaxies we were able to recover their star formation history (up to ∼ 2 − 3 Gyr ago since we do not observe the oldest main sequence turn-off or horizontal branch, due to the systems’ distance), finding that the SF never really stopped, but proceeded continuously even with the succession of high and low activity. The time intervals where we find higher SF rates in the two galaxies well agree with the dynamical timescales of previous interactions events, which might represent a major channel for triggering the SF in relatively isolated galaxies.
What do planetary nebulae and H II regions reveal about the chemical evolution of nearby dwarf galaxies?
Authors: Gonçalves Denise R.
Published: October 2019

The Local Group contains a great number of dwarf irregulars and spheroidals, for which the spectroscopy of individual stars can be obtained. Thus, the chemical evolution of these galaxies can be traced, with the only need of finding populations spanning a large age range and such that we can accurately derive the composition. Planetary nebulae (PNe) are old- and intermediate-age star remnants and their chemical abundances can be obtained up to 3-4 Mpc. H ii regions, which are brighter and much easily detected, represent galaxies young content. PNe and H ii regions share similar spectroscopic features and are analysed in the same way. Both are among the best tracers of the chemical evolution allowing to draw the chemical time line of nearby galaxies. The focus in this review are the PN and H ii region populations as constraints to the chemical evolution models and the mass-metallicity relation of the local universe.
Naloxon – Medizinische Grundlagen und internationale Erfahrungen
Authors: Wodarz Norbert , Wolstein Jörg , Essen Heike Wodarz-von , Pogarell Oliver
Published: October 2019

Zusammenfassung. Hintergrund: Die Abhängigkeit von Opioiden ist mit erheblichen gesundheitlichen Gefährdungen der Betroffenen und einer hohen Mortalität assoziiert. Derzeit werden insbesondere die dramatisch gestiegenen Mortalitätsraten in den USA diskutiert (‚opioid crisis‘), aber auch in Deutschland war in den letzten Jahren ein kontinuierlicher Anstieg der Drogentoten, überwiegend verursacht durch Opioide, zu verzeichnen. Die Risiken einer Opioid Überdosierung bzw. Intoxikation resultiert vor allem aus der hemmenden Wirkung der Opioide auf den Atemantrieb, die durch andere gleichzeitig konsumierte Substanzen noch verstärkt werden kann. Neben Erstmaßnahmen (Notruf, lebensrettende Basismaßnahmen der Ersten Hilfe) kommt auch der Einsatz des Opioidantagonisten Naloxon in Betracht. Methode: Literaturrecherche in PubMed, Cochrane Library und im International Standard Randomized Controlled Trial Number (ISRCTN) Register. Ergebnisse: Auch aus ethischen Gründen liegen bislang eher wenig systematische Untersuchungen zum nicht-ärztlichen Einsatz von Naloxon bei Opioidüberdosierung vor. Trotzdem kann nach aktuellem Stand geschlussfolgert werden, dass die intranasale Verabreichung vergleichbar wirksam mit einer intramuskulären Anwendung zu sein scheint. Bei Überdosierten, die nach erfolgreichem Naloxon-Einsatz aus unterschiedlichen Gründen nicht in einer Klinik gebracht wurden, wurden in ca. 1 %. Todesfälle dokumentiert. Falls 60 min nach Naloxongabe unauffällige Vitalparameter und auf der Glasgow Coma Scale mind. 15 Punkte erreicht werden, besteht wohl ein sehr niedriges Rebound-Risiko. Im Vergleich dazu ist das Auftreten von Naloxon-induzierten Entzugssyndromen deutlich häufiger, hängen jedoch von der Dosis und dem konsumierten Opioid ab, wie auch von der verabreichten Naloxon-Dosis. Schlussfolgerungen: Naloxon kann mittlerweile auch in Deutschland als zugelassenes Nasalspray verabreicht werden und ist daher im Prinzip auch für den Einsatz durch Ersthelfer geeignet. Verbesserte Rahmenbedingungen, wie z. B. spezifische Schulungen könnten dazu beitragen, Take-Home Naloxon als erfolgreichen Baustein zur Reduktion von Drogentod zu implementieren.
Kognitive Verhaltenstherapie bei Bulimia nervosa
Authors: Terpitz Corinna
Published: October 2019

Zusammenfassung. Die kognitive Verhaltenstherapie für Essstörungen ist eine evidenzbasierte wirksame Methode zur Behandlung der Bulimia nervosa. Sie basiert auf einem multifaktoriellen und lerntheoretischen Störungsmodell, in dem Ursachen, auslösende und aufrechterhaltende Bedingungen berücksichtigt und in einer Verhaltensanalyse zusammengefasst werden. Im Therapieprozess kommen symptomorientierte Standardverfahren zum Einsatz. Es werden Fertigkeiten wie Emotionsregulation, interpersonelle Kompetenz und kognitive Techniken vermittelt. Einen besonderen Stellenwert haben dabei der niedrige Selbstwert und die Körperunzufriedenheit. Schliesslich wird mit den Betroffenen unter Einbezug von individuellen Bedürfnissen an persönlichen Zielen gearbeitet.
Discovery of autism/intellectual disability somatic mutations in Alzheimer's brains: mutated ADNP cytoskeletal impairments and repair as a case study
Authors: Ivashko-Pachima Yanina , Hadar Adva , Grigg Iris , Korenková Vlasta , Kapitansky Oxana , Karmon Gidon , Gershovits Michael , Sayas C. Laura , Kooy R. Frank , Attems Johannes , Gurwitz David , Gozes Illana
Published: October 2019

With Alzheimer’s disease (AD) exhibiting reduced ability of neural stem cell renewal, we hypothesized that de novo mutations controlling embryonic development, in the form of brain somatic mutations instigate the disease. A leading gene presenting heterozygous dominant de novo autism-intellectual disabilities (ID) causing mutations is activity-dependent neuroprotective protein (ADNP), with intact ADNP protecting against AD-tauopathy. We discovered a genomic autism ADNP mutation (c.2188C>T) in postmortem AD olfactory bulbs and hippocampi. RNA-Seq of olfactory bulbs also identified a novel ADNP hotspot mutation, c.2187_2188insA. Altogether, 665 mutations in 596 genes with 441 mutations in AD patients (389 genes, 38% AD—exclusive mutations) and 104 genes presenting disease-causing mutations (OMIM) were discovered. OMIM AD mutated genes converged on cytoskeletal mechanisms, autism and ID causing mutations (about 40% each). The number and average frequencies of AD-related mutations per subject were higher in AD subjects compared to controls. RNA-seq datamining (hippocampus, dorsolateral prefrontal cortex, fusiform gyrus and superior frontal gyrus—583 subjects) yielded similar results. Overlapping all tested brain areas identified unique and shared mutations, with ADNP singled out as a gene associated with autism/ID/AD and presenting several unique aging/AD mutations. The large fusiform gyrus library (117 subjects) with high sequencing coverage correlated the c.2187_2188insA ADNP mutation frequency to Braak stage (tauopathy) and showed more ADNP mutations in AD specimens. In cell cultures, the ADNP-derived snippet NAP inhibited mutated-ADNP-microtubule (MT) toxicity and enhanced Tau–MT association. We propose a paradigm-shifting concept in the perception of AD whereby accumulating mosaic somatic mutations promote brain pathology.
Single-molecule sensing of peptides and nucleic acids by engineered aerolysin nanopores
Authors: Cao Chan , Cirauqui Nuria , Marcaida Maria Jose , Buglakova Elena , Duperrex Alice , Radenovic Aleksandra , Dal Peraro Matteo
Published: October 2019

Nanopore sensing is a powerful single-molecule approach for the detection of biomolecules. Recent studies have demonstrated that aerolysin is a promising candidate to improve the accuracy of DNA sequencing and to develop novel single-molecule proteomic strategies. However, the structure–function relationship between the aerolysin nanopore and its molecular sensing properties remains insufficiently explored. Herein, a set of mutated pores were rationally designed and evaluated in silico by molecular simulations and in vitro by single-channel recording and molecular translocation experiments to study the pore structural variation, ion selectivity, ionic conductance and capabilities for sensing several biomolecules. Our results show that the ion selectivity and sensing ability of aerolysin are mostly controlled by electrostatics and the narrow diameter of the double β-barrel cap. By engineering single-site mutants, a more accurate molecular detection of nucleic acids and peptides has been achieved. These findings open avenues for developing aerolysin nanopores into powerful sensing devices.
Unstable TTTTA/TTTCA expansions in MARCH6 are associated with Familial Adult Myoclonic Epilepsy type 3
Authors: Florian Rahel T. , Kraft Florian , Leitão Elsa , Kaya Sabine , Klebe Stephan , Magnin Eloi , van Rootselaar Anne-Fleur , Buratti Julien , Kühnel Theresa , Schröder Christopher , Giesselmann Sebastian , Tschernoster Nikolai , Altmueller Janine , Lamiral Anaide , Keren Boris , Nava Caroline , Bouteiller Delphine , Forlani Sylvie , Jornea Ludmila , Kubica Regina , Ye Tao , Plassard Damien , Jost Bernard , Meyer Vincent , Deleuze Jean-François , Delpu Yannick , Avarello Mario D. M. , Vijfhuizen Lisanne S. , Rudolf Gabrielle , Hirsch Edouard , Kroes Thessa , Reif Philipp S. , Rosenow Felix , Ganos Christos , Vidailhet Marie , Thivard Lionel , Mathieu Alexandre , Bourgeron Thomas , Kurth Ingo , Rafehi Haloom , Steenpass Laura , Horsthemke Bernhard ,   FAME consortium, Berkovic Samuel F. , Bisulli Francesca , Brancati Francesco , Canafoglia Laura , Casari Giorgio , Guerrini Renzo , Ishiura Hiroyuki , Licchetta Laura , Mei Davide , Pippucci Tommaso , Sadleir Lynette , Scheffer Ingrid E. , Striano Pasquale , Tinuper Paolo , Tsuji Shoji , Zara Federico , LeGuern Eric , Klein Karl Martin , Labauge Pierre , Bennett Mark F. , Bahlo Melanie , Gecz Jozef , Corbett Mark A. , Tijssen Marina A. J. , van den Maagdenberg Arn M. J. M. , Depienne Christel
Published: October 2019

Familial Adult Myoclonic Epilepsy (FAME) is a genetically heterogeneous disorder characterized by cortical tremor and seizures. Intronic TTTTA/TTTCA repeat expansions in SAMD12 (FAME1) are the main cause of FAME in Asia. Using genome sequencing and repeat-primed PCR, we identify another site of this repeat expansion, in MARCH6 (FAME3) in four European families. Analysis of single DNA molecules with nanopore sequencing and molecular combing show that expansions range from 3.3 to 14 kb on average. However, we observe considerable variability in expansion length and structure, supporting the existence of multiple expansion configurations in blood cells and fibroblasts of the same individual. Moreover, the largest expansions are associated with micro-rearrangements occurring near the expansion in 20% of cells. This study provides further evidence that FAME is caused by intronic TTTTA/TTTCA expansions in distinct genes and reveals that expansions exhibit an unexpectedly high somatic instability that can ultimately result in genomic rearrangements.
Automatic structure-based NMR methyl resonance assignment in large proteins
Authors: Pritišanac Iva , Würz Julia M. , Alderson T. Reid , Güntert Peter
Published: October 2019

Isotopically labeled methyl groups provide NMR probes in large, otherwise deuterated proteins. However, the resonance assignment constitutes a bottleneck for broader applicability of methyl-based NMR. Here, we present the automated MethylFLYA method for the assignment of methyl groups that is based on methyl-methyl nuclear Overhauser effect spectroscopy (NOESY) peak lists. MethylFLYA is applied to five proteins (28–358 kDa) comprising a total of 708 isotope-labeled methyl groups, of which 612 contribute NOESY cross peaks. MethylFLYA confidently assigns 488 methyl groups, i.e. 80% of those with NOESY data. Of these, 459 agree with the reference, 6 were different, and 23 were without reference assignment. MethylFLYA assigns significantly more methyl groups than alternative algorithms, has an average error rate of 1%, modest runtimes of 0.4–1.2 h, and can handle arbitrary isotope labeling patterns and data from other types of NMR spectra.
E2F1 acetylation directs p300/CBP-mediated histone acetylation at DNA double-strand breaks to facilitate repair
Authors: Manickavinayaham Swarnalatha , Vélez-Cruz Renier , Biswas Anup K. , Bedford Ella , Klein Brianna J. , Kutateladze Tatiana G. , Liu Bin , Bedford Mark T. , Johnson David G.
Published: October 2019

E2F1 and retinoblastoma (RB) tumor-suppressor protein not only regulate the periodic expression of genes important for cell proliferation, but also localize to DNA double-strand breaks (DSBs) to promote repair. E2F1 is acetylated in response to DNA damage but the role this plays in DNA repair is unknown. Here we demonstrate that E2F1 acetylation creates a binding motif for the bromodomains of the p300/KAT3B and CBP/KAT3A acetyltransferases and that this interaction is required for the recruitment of p300 and CBP to DSBs and the induction of histone acetylation at sites of damage. A knock-in mutation that blocks E2F1 acetylation abolishes the recruitment of p300 and CBP to DSBs and also the accumulation of other chromatin modifying activities and repair factors, including Tip60, BRG1 and NBS1, and renders mice hypersensitive to ionizing radiation (IR). These findings reveal an important role for E2F1 acetylation in orchestrating the remodeling of chromatin structure at DSBs to facilitate repair.
Shape-programmed 3D printed swimming microtori for the transport of passive and active agents
Authors: Baker Remmi Danae , Montenegro-Johnson Thomas , Sediako Anton D. , Thomson Murray J. , Sen Ayusman , Lauga Eric , Aranson Igor. S.
Published: October 2019

Through billions of years of evolution, microorganisms mastered unique swimming behaviors to thrive in complex fluid environments. Limitations in nanofabrication have thus far hindered the ability to design and program synthetic swimmers with the same abilities. Here we encode multi-behavioral responses in microscopic self-propelled tori using nanoscale 3D printing. We show experimentally and theoretically that the tori continuously transition between two primary swimming modes in response to a magnetic field. The tori also manipulated and transported other artificial swimmers, bimetallic nanorods, as well as passive colloidal particles. In the first behavioral mode, the tori accumulated and transported nanorods; in the second mode, nanorods aligned along the toriʼs self-generated streamlines. Our results indicate that such shape-programmed microswimmers have a potential to manipulate biological active matter, e.g. bacteria or cells.
Goal congruency dominates reward value in accounting for behavioral and neural correlates of value-based decision-making
Authors: Frömer Romy , Dean Wolf Carolyn K. , Shenhav Amitai
Published: October 2019

When choosing between options, whether menu items or career paths, we can evaluate how rewarding each one will be, or how congruent it is with our current choice goal (e.g., to point out the best option or the worst one.). Past decision-making research interpreted findings through the former lens, but in these experiments the most rewarding option was always most congruent with the task goal (choosing the best option). It is therefore unclear to what extent expected reward vs. goal congruency can account for choice value findings. To deconfound these two variables, we performed three behavioral studies and an fMRI study in which the task goal varied between identifying the best vs. the worst option. Contrary to prevailing accounts, we find that goal congruency dominates choice behavior and neural activity. We separately identify dissociable signals of expected reward. Our findings call for a reinterpretation of previous research on value-based choice.
Author Correction: De novo phased assembly of the Vitis riparia grape genome
Authors: Girollet Nabil , Rubio Bernadette , Lopez-Roques Céline , Valière Sophie , Ollat Nathalie , Bert Pierre-François
Published: October 2019

An amendment to this paper has been published and can be accessed via a link at the top of the paper