bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimization of the Decolorization of the Reactive Black 5 by a Laccase-like Active Cell-Free Supernatant from Coriolopsis gallica
Authors: Ben Ayed Amal , Hadrich Bilel , Sciara Giuliano , Lomascolo Anne , Bertrand Emmanuel , Faulds Craig B. , Zouari-Mechichi Héla , Record Eric , Mechichi Tahar
Published: June 2022

The textile industry generates huge volumes of colored wastewater that require multiple treatments to remove persistent toxic and carcinogenic dyes. Here we studied the decolorization of a recalcitrant azo dye, Reactive Black 5, using laccase-like active cell-free supernatant from Coriolopsis gallica. Decolorization was optimized in a 1 mL reaction mixture using the response surface methodology (RSM) to test the influence of five variables, i.e., laccase-like activity, dye concentration, redox mediator (HBT) concentration, pH, and temperature, on dye decolorization. Statistical tests were used to determine regression coefficients and the quality of the models used, as well as significant factors and/or factor interactions. Maximum decolorization was achieved at 120 min (82 ± 0.6%) with the optimized protocol, i.e., laccase-like activity at 0.5 U mL−1, dye at 25 mg L−1, HBT at 4.5 mM, pH at 4.2 and temperature at 55 °C. The model proved significant (ANOVA test with p < 0.001): coefficient of determination (R²) was 89.78%, adjusted coefficient of determination (R²A) was 87.85%, and root mean square error (RMSE) was 10.48%. The reaction conditions yielding maximum decolorization were tested in a larger volume of 500 mL reaction mixture. Under these conditions, the decolorization rate reached 77.6 ± 0.4%, which was in good agreement with the value found on the 1 mL scale. RB5 decolorization was further evaluated using the UV-visible spectra of the treated and untreated dyes.
Dark Diazotrophy during the Late Summer in Surface Waters of Chile Bay, West Antarctic Peninsula
Authors: Alcamán-Arias María E. , Cifuentes-Anticevic Jerónimo , Castillo-Inaipil Wilson , Farías Laura , Sanhueza Cynthia , Fernández-Gómez Beatriz , Verdugo Josefa , Abarzua Leslie , Ridley Christina , Tamayo-Leiva Javier , Díez Beatriz
Published: June 2022

Although crucial for the addition of new nitrogen in marine ecosystems, dinitrogen (N2) fixation remains an understudied process, especially under dark conditions and in polar coastal areas, such as the West Antarctic Peninsula (WAP). New measurements of light and dark N2 fixation rates in parallel with carbon (C) fixation rates, as well as analysis of the genetic marker nifH for diazotrophic organisms, were conducted during the late summer in the coastal waters of Chile Bay, South Shetland Islands, WAP. During six late summers (February 2013 to 2019), Chile Bay was characterized by high NO3− concentrations (~20 µM) and an NH4+ content that remained stable near 0.5 µM. The N:P ratio was approximately 14.1, thus close to that of the Redfield ratio (16:1). The presence of Cluster I and Cluster III nifH gene sequences closely related to Alpha-, Delta- and, to a lesser extent, Gammaproteobacteria, suggests that chemosynthetic and heterotrophic bacteria are primarily responsible for N2 fixation in the bay. Photosynthetic carbon assimilation ranged from 51.18 to 1471 nmol C L−1 d−1, while dark chemosynthesis ranged from 9.24 to 805 nmol C L−1 d−1. N2 fixation rates were higher under dark conditions (up to 45.40 nmol N L−1 d−1) than under light conditions (up to 7.70 nmol N L−1 d−1), possibly contributing more than 37% to new nitrogen-based production (≥2.5 g N m−2 y−1). Of all the environmental factors measured, only PO43- exhibited a significant correlation with C and N2 rates, being negatively correlated (p < 0.05) with dark chemosynthesis and N2 fixation under the light condition, revealing the importance of the N:P ratio for these processes in Chile Bay. This significant contribution of N2 fixation expands the ubiquity and biological potential of these marine chemosynthetic diazotrophs. As such, this process should be considered along with the entire N cycle when further reviewing highly productive Antarctic coastal waters and the diazotrophic potential of the global marine ecosystem.
Comparative Phenotypic and Genomic Features of Staphylococci from Sonication Fluid of Orthopedic Implant-Associated Infections with Poor Outcome
Authors: Santos Ingrid Nayara Marcelino , Kurihara Mariana Neri Lucas , Santos Fernanda Fernandes , Valiatti Tiago Barcelos , Silva Juliana Thalita Paulino da , Pignatari Antônio Carlos Campos , Salles Mauro José
Published: June 2022

Staphylococcus spp. remain the leading biofilm-forming agents causing orthopedic implant-associated infections (OIAI). This is a descriptive study of phenotypic and genomic features identified in clinical isolates of S. aureus and coagulase-negative Staphylococcus (CoNS) recovered from OIAIs patients that progressed to treatment failure. Ten isolates were identified by matrix-time-of-flight laser-assisted desorption mass spectrometry (MALDI-TOF-MS) and tested for antibiotic susceptibility and biofilm formation. Genotypic characteristics, including, MLST (Multi Locus Sequence Typing), SCCmec typing, virulence and resistance genes were assessed by whole-genome sequencing (WGS). All S. aureus harbored mecA, blaZ, and multiple resistance genes for aminoglycosides and quinolones. All MRSA were strong biofilm producers harboring the complete icaADBC and icaR operon. Seven CoNS isolates comprising five species (S. epidermidis, S. haemolyticus, S. sciuri, S. capitis and S. lugdunensis) were analyzed, with mecA gene detected in five isolates. S. haemolitycus (isolate 95), and S. lugdunensis were unable to form biofilm and did not harbor the complete icaADBCR operon. High variability of adhesion genes was detected, with atl, ebp, icaADBC operon, and IS256 being the most common. In conclusion, MRSA and CoNS isolates carrying genes for biofilm production, and resistance to β-lactam and aminoglycosides are associated with treatment failure in OIAIs.
Using Genomics to Design a Pathovar-Specific Loop-Mediated Isothermal Amplification (LAMP) Assay, for the Improved Detection of Xanthomonas citri pv. citri
Authors: Webster John , Kehoe Monica A. , Nogarotto Elisse , Falconer Linda , Donovan Nerida Jane , Chapman Toni A.
Published: June 2022

The ability to swiftly respond to pathogen incursions relies heavily on fast and accurate diagnostics. Current published assays for citrus bacterial canker do not target Xanthomonas citri pv. citri, the causative agent, with high specificity when testing Australian samples. While the current diagnostics are useful in countries where canker is endemic, the detection of canker in Australia requires an emergency response. Close relatives to X. citri pv. citri found in Australia may generate false positives with the current recommended diagnostic assays. Therefore, we developed a more specific detection tool for citrus bacterial canker to provide greater diagnostic confidence for surveillance and eradication efforts. We used genomic comparisons of 161 Xanthomonad genomes and identified and confirmed genomic regions specific for X. citri pv. citri by performing local alignments of unique regions to reference genomes. We then developed loop-mediated isothermal amplification primers and validated them against a panel of 190 isolates to confirm specificity. Our diagnostic assay showed 100% corroboration with the concurrently developed multiplex primers and represents an improved diagnostic method capable of effective citrus bacterial canker identification.
Microbial Interrelationships across Sites of Breastfeeding Mothers and Infants at 6 Weeks Postpartum
Authors: Davis Erin C. , Wang Mei , Donovan Sharon M.
Published: June 2022

Infancy is a critical life stage for the establishment of the gut microbiome. Human milk contains a unique microbial ecosystem that serves as a continuous source of commensal bacteria for the infant. However, the origin of the human milk microbiota, how it is influenced by breastfeeding exclusivity, and its role in infant gut microbiota assembly are not clear. To interrogate these questions, we examined the relationships among fecal, oral, breast skin, and human milk microbiota of 33 exclusively breastfeeding (EBF) and mixed-feeding (MF; human milk + infant formula) mother–infant pairs at 6 weeks postpartum. Here, we show that MF infants have a significantly more diverse oral microbiome comprised of lower relative abundances of Streptococcus and Gemella and higher abundances of Veillonella. Using both SourceTracker2 and FEAST, we demonstrate breast skin and infant saliva as the principal contributing sources to the human milk microbiota. Of the sampled sites, human milk and maternal stool were predicted to contribute the largest fraction to the infant fecal microbiome, but the majority of the community was estimated to arise from unknown sources. Lastly, we identified twenty-one significant co-occurrence relationships between bacteria in human milk and on other maternal and infant body sites. These results demonstrate several unique microbial interrelationships between breastfeeding dyads, providing insight into potential mechanisms of microbial assembly in early life.
Correction: Döring et al. Discovery of Novel Symmetrical 1,4-Dihydropyridines as Inhibitors of Multidrug-Resistant Protein (MRP4) Efflux Pump for Anticancer Therapy. Molecules 2021, 26, 18
Authors: Döring Henry , Kreutzer David , Ritter Christoph , Hilgeroth Andreas
Published: June 2022

The authors would like to correct an error made through no fault of their own in the title paper [...]
Warning Signs of High-Pressure Formations of Abnormal Contour Pressures When Drilling for Oil and Natural Gas
Authors: Huszar Tomas , Wittenberger Gabriel , Skvarekova Erika
Published: June 2022

When drilling to obtain hydrocarbons (oil and natural gas), we cannot underestimate the anomalously high pressures in the deposit layers, as these pressures can cause an uncontrollable eruption. Therefore, it is important to look for signs of anomalous high contour pressures over time, which, according to a detailed analysis, could be used to predict and quantify high formation pressures. These arise under conditions of intense vertical migration of formation fluids, where the liquids in the well have to carry part of the weight of overlying rocks and are often also related to tectonic activity. The main aim of the present study was to detect the emergence of a gas kick, which, as a result of an improper technological procedure, can cause an uncontrollable eruption, which can lead to a total accident of the well. In this article, we describe the use of modern drilling technology and sophisticated software that displays the current status inside the well. These can reveal impending pressure anomalies that can cause complications in managing the gas kick in oil and natural gas drilling. We analysed the most appropriate procedure for well control in a hydrocarbon well using the “driller’s method” and the “wait and weight method”. On the basis of theoretical background, we verified the correctness of the procedure for well control and compared it with the reaction to gas kick from a well drilled in Hungary. In the article, we highlight mistakes, as well as the particular importance of properly managing gas kick and its early prediction. Proper management of gas kick and its early prediction highlight the particular importance of implementing safe and effective procedures in well drilling.
Bioactive Peptides: An Understanding from Current Screening Methodology
Authors: Nong Nhung Thi Phuong , Hsu Jue-Liang
Published: June 2022

Bioactive peptides with high potency against numerous human disorders have been regarded as a promising therapy in disease control. These peptides could be released from various dietary protein sources through hydrolysis processing using physical conditions, chemical agents, microbial fermentation, or enzymatic digestions. Considering the diversity of the original proteins and the complexity of the multiple structural peptides that existed in the hydrolysis mixture, the screening of bioactive peptides will be a challenge task. Well-organized and well-designed methods are necessarily required to enhance the efficiency of studying the potential peptides. This article, hence, provides an overview of bioactive peptides with an emphasis on the current strategy used for screening and characterization methods. Moreover, the understanding of the biological activities of peptides, mechanism inhibitions, and the interaction of the complex of peptide–enzyme is commonly evaluated using specific in vitro assays and molecular docking analysis.
The Effect of pH Solution in the Sol–Gel Process on the Structure and Properties of Thin SnO2 Films
Authors: Murzalinov Danatbek , Dmitriyeva Elena , Lebedev Igor , Bondar Ekaterina A. , Fedosimova Anastasiya I. , Kemelbekova Ainagul
Published: June 2022

The synthesis of surface-active structures is important for creating many applications. The structural formation of SnO2 thin films in the range from 1.4 to 1.53 pH is studied in this work. This process occurs on the surface of the sample in the range of 1.4 to 1.49 and in the volume in the range of 1.51 to 1.53. SnO2 is formed after annealing at 400 ∘C, according to XRD. Doping NH4OH to solution stimulates particle coagulation and gel formation. All of these have an impact on the transparency of samples investigated by spectrophotometric methods. By increasing the pH, the resistance raises at room temperature. The Eg calculation along the fundamental absorption edge shows that it is greater than 3.6 eV’ for SnO2 films. According to the Burstein–Moss effect, a change of the bandgap is related to the increased concentration of the free charge carriers. Elemental analysis has shown that chlorine ions are considered to be additional sources of charge carriers. The value pH = 1.49 is critical since there is a drastic change in the structure of the samples, the decrease in transparency is replaced by its increase, and the energy of activation of impurity levels is changed.
Dysregulated Gene Expression in Lymphoblasts from Parkinson’s Disease
Authors: Annesley Sarah Jane , Allan Claire Yvonne , Sanislav Oana , Evans Andrew , Fisher Paul Robert
Published: June 2022

Parkinson’s disease is the second largest neurodegenerative disease worldwide and is caused by a combination of genetics and environment. It is characterized by the death of neurons in the substantia nigra of the brain but is not solely a disease of the brain, as it affects multiple tissues and organs. Studying Parkinson’s disease in accessible tissues such as skin and blood has increased our understanding of the disease’s pathogenesis. Here, we used lymphoblast cell lines generated from Parkinson’s disease patient and healthy age- and sex-matched control groups and obtained their whole-cell transcriptomes and proteomes. Our analysis revealed, in both the transcriptomes and the proteomes of PD cells, a global downregulation of genes involved in protein synthesis, as well as the upregulation of immune processes and sphingolipid metabolism. In contrast, we discovered an uncoupling of mRNA and protein expression in processes associated with mitochondrial respiration in the form of a general downregulation in associated transcripts and an upregulation in proteins. Complex V was different to the other oxidative phosphorylation complexes in that the levels of its associated transcripts were also lower, but the levels of their encoded polypeptides were not elevated. This may suggest that further layers of regulation specific to Complex V are in play.
Demonstration of Neutron Phase Imaging Based on Talbot–Lau Interferometer at Compact Neutron Source RANS
Authors: Takano Hidekazu , Wu Yanlin , Samoto Tetsuo , Taketani Atsushi , Takanashi Takaoki , Iwamoto Chihiro , Otake Yoshie , Momose Atsushi
Published: June 2022

Neutron imaging based on a compact Talbot–Lau interferometer was demonstrated using the RIKEN accelerator-driven compact neutron source (RANS). A compact Talbot–Lau interferometer consisting of gadolinium absorption gratings and a silicon phase grating was constructed and connected to the RANS. Because of pulsed thermal neutrons from the RANS and a position-sensitive detector equipped with time-of-flight (TOF) analysis, moiré interference patterns generated using the interferometer were extracted at a TOF range around the design wavelength (2.37 Å) optimal for the interferometer. Differential phase and scattering images of the metal rod samples were obtained through phase-stepping measurements with the interferometer. This demonstrates the feasibility of neutron phase imaging using a compact neutron facility and the potential for flexible and unique applications for nondestructive evaluation.
Antistars or Antimatter Cores in Mirror Neutron Stars?
Authors: Berezhiani Zurab
Published: June 2022

The oscillation of the neutron n into mirror neutron n′, its partner from the dark mirror sector, can gradually transform an ordinary neutron star into a mixed star consisting in part of mirror dark matter. The implications of the reverse process taking place in the mirror neutron stars depend on the sign of baryon asymmetry in the mirror sector. Namely, if it is negative, as predicted by certain baryogenesis scenarios, then n′¯−n¯ transitions create a core of our antimatter gravitationally trapped in the mirror star interior. The annihilation of accreted gas on such antimatter cores could explain the origin of γ-source candidates with an unusual spectrum compatible with baryon–antibaryon annihilation, recently identified in the Fermi LAT catalog. In addition, some part of this antimatter escaping after the mergers of mirror neutron stars can produce the flux of cosmic antihelium and also heavier antinuclei which are hunted in the AMS-02 experiment.
Antimicrobial Resistant Salmonella in Chelonians: Assessing Its Potential Risk in Zoological Institutions in Spain
Authors: Marin Clara , Martín-Maldonado Bárbara , Cerdà-Cuéllar Marta , Sevilla-Navarro Sandra , Lorenzo-Rebenaque Laura , Montoro-Dasi Laura , Manzanares Alicia , Ayats Teresa , Mencía-Gutiérrez Aida , Jordá Jaume , González Fernando , Rojo-Solís Carlos , Barros Carlos , García-Párraga Daniel , Vega Santiago
Published: June 2022

Salmonella is mostly noted as a food-borne pathogen, but contact with chelonians has also been reported as a source of infection. Moreover, high levels of antimicrobial resistance (AMR) have been reported in Salmonella isolated from wild and captive reptiles. The aim of this study was to assess the occurrence of Salmonella AMR carriage by chelonians admitted to two zoological institutions in Spain, characterizing the isolates to assess the Salmonella AMR epidemiology in wildlife. To this end, 152 chelonians from nine species were sampled upon their arrival at the zoological nuclei. Salmonella identification was based on ISO 6579-1:2017 (Annex D), isolates were serotyped and their AMR analysed according to the EU Decision 2013/652. Moreover, the genetic relationship of the isolates was assessed by pulsed-field gel electrophoresis (PFGE). Results showed 19% (29/152) of the chelonians positive to Salmonella, all of them tortoises. For all isolates, 69% (20/29) were resistant and 34% (10/29) multidrug-resistant (MDR) strains. PFGE clustered isolates according to the serovar, confirming a low genetic diversity. In conclusion, this study shows a high presence of MDR Salmonella strains in tortoises at their entry into zoological nuclei. This condition highlights the need to establish Salmonella detection protocols for the entry of animals into these centres.
Brazilian Organic Honey from Atlantic Rainforest Decreases Inflammatory Process in Mice
Authors: Romário-Silva Diego , Lazarini Josy Goldoni , Franchin Marcelo , de Alencar Severino Matias , Rosalen Pedro Luiz
Published: June 2022

Honey is an ancient food in the human diet, and the chemical composition of some types of honey has been associated with several beneficial biological effects. Among them, honey has been highlighted to improve health and control inflammatory processes. However, there is no study elucidating the mechanism of action of honey produced organically. Here, we separated organic honey (OH) samples from the Brazilian Atlantic Rainforest into eight different profiles (OH-1 to OH-8) and evaluated, in vitro and in vivo, their anti-inflammatory potential. To determine cell viability, RAW 264.7 macrophages were treated with several concentrations of OH-1 up to OH-8, and anti-inflammatory activity was assessed through NF-κB activation and TNF-α levels. All types of the studied honey up to a concentration of 4% (w/v) did not interfere with macrophage viability and decreased NF-kB activation and TNF-α levels in macrophage culture in vitro. OH-7 was selected as the most promising anti-inflammatory and used in subsequent assays. Mice pretreated orally with OH-7 showed a decrease in neutrophil migration and TNF-α level. Thus, these types of Brazilian organic honey show promising anti-inflammatory potential, particularly the OH-7 variety. Brazilian organic honey may lead to the development of new products and/or be incorporated into food for use in veterinary medicine and human health as well.
On the use of integral indicators of the functional state of the children’s body as criteria for realizing the risk of adverse effects of environmental factors
Authors:   ederal State Budgetary Educational Institution of Higher Education «Orenburg State Medical University» of the Ministry of Health of the Russian Federation, Bulycheva Ekaterina Vladimirovna
Published: June 2022

Special attention is always paid to the health of children associated with the influence of environmental factors in scientific research. This is due to the fact that environmental factors play an important role in determining the health and well-being of children. Of particular importance is the search for informative integral indicators that characterize the impact of various degrees of anthropogenic load on the health of the child population. The article presents the results of a study of integral indicators of the health status of 315 children aged 4–6 years living in the territory with a high (group 1) and low (group 2) level of anthropogenic load, such as the stability of the nervous reaction, the stress index of regulatory systems, the index of vegetative equilibrium, the vegetative rhythm indicator, the indicator of the adequacy of regulatory processes. The obtained data indicate that a high level of anthropogenic load has an adverse effect on the integral indicators of the functional state of the central nervous, autonomic and cardiovascular systems. This is characterized by a decrease in the stability index of the nervous reaction, an increase in the sympathetic activity of the central nervous system, depletion of the central regulatory circuits due to prolonged activation of non-economic mechanisms for regulating the high tone of the sympathetic nervous system. At the same time, the adverse effects increase with an increase in the age of children from 4 to 6 years old living in an area with a high level of anthropogenic load. The established facts of changes in integral indicators represent a scientific justification for the use of these indicators in the development of mathematical models for managing risks to children’s health under the influence of anthropogenic factors.
Use of acid-suppressive drugs in gastrointestinal tract lesions in COVID-19
Authors:   FSBEI HE Volgograd State Medical University, Skvortsov Vsevolod Vladimirovich , Ponomareva Anastasiya Romanovna ,   FSBEI HE Volgograd State Medical University, Skvortsova Ekaterina Mikhailovna ,   FSBEI HE Volgograd State Medical University
Published: June 2022

This review is devoted to the clinical picture, approaches to the diagnosis and treatment of gastrointestinal diseases in the novel coronavirus SARS-CoV-2.
Case study: Knowledge, attitude, and practice (KAP) regarding hearing protection: A case study among offset printing industry workers in Tehran province
Authors: Vosoughi Shahram , Alimohammadi Iraj , Hosseini Agha Fatemeh , Noushabadi Zhaleh Sedghi
Published: June 2022

The aim of this study is to determine the relationship between knowledge and attitude toward using hearing protection devices as a safe behavior. Also, it is intended to analyze the role of individuals and job characteristics on using earplugs or earmuffs. In this study, workers participated
from 16 printing industries located in five areas of Tehran province and completed the questionnaire knowledge, attitude, and practice (KAP) about hearing conservation. Noise measurement was performed based on participants' tasks. With increasing attitude, practice improved (P = 0.001). The
results revealed that positive attitude led to improved practice of workers in hearing conservation (r = 0.172; P < 0.001), and with increasing knowledge, negative attitude diminished (P=0.014). The results indicated that awareness is correlated with attitude, and attitude could directly
affect the behavior. Thus, awareness as an intermediate or primary factor might play an important role in developing safe behaviors.
Uncertainty of in situ low frequency reverberation time measurements from 20 Hz – An empirical study; Part II: Impulse response method
Authors: Ljunggren Fredrik , Simmons Christian , Pettersson Maria
Published: June 2022

An important part of evaluating impact sound insulation is to measure the reverberation time in the receiving room. The increasing trend of using wooden structures for residential buildings has made it increasingly important to have control of the measurement procedure at low frequencies.
The present ISO standards do not give any specific guidelines for how to deal with frequencies below 50 Hz. It cannot be taken for granted that the present guidelines developed for higher frequencies are also appropriate for lower frequencies. This paper includes an empirical study of the
spatial variation of reverberation time, measured from 20 Hz in two different rooms, with about 100 microphone positions in each room. The results from two alternative methods are compared, based upon the impulse response and the interrupted noise respectively. The accuracy of an intended
normal measurement procedure is estimated with respect to the number of microphone positions.
PENERAPAN DESAIN SISTEM MENGGUNAKAN METODE ATOMIC DESIGN DI UNIVERSITAS MUHAMMADIYAH SUKABUMI
Authors: Kurniawan Hari , Adiwijaya Fakhrian Fadlia
Published: June 2022

Universitas Muhammadiah Sukabumi (UMMI) merupakan instansi yang bergerak di bidang pendidikan, khususnya perguruan tinggi. Ummi memiliki berbagai website yang digunakan sebagai perantara antara mahasiswa dengan pihak universitas yang memiliki karakteristik dan kebutuhan yang berbeda. Dalam pengembangan websitenya terdapat perbedaan pendapat antara pihak universitas sebagai pemangku keputusan dengan pihak pengembang websitenya yang menyebabkan ketidak konsistenan tampilan antarmuka dan lamanya proses pembuatan desain karena desainer selalu membuat konsep desai dari awal. design system merupakan guidline dan dokumentasi yang terdiri dari kumpulan komponen user interface (UI) yang saling terhubung, metode yang digunakan menggunakan atomic design. dengan menggunakan design system diharapkan ketidak konsistenan dalam mengembangkan produk dan lamanya proses desain di Ummi dapat diatasi. Berdasarkan hasil pengujian waktu keseluruhan yang dibutuhkan dalam menyelesaikan tugas setelah design system diterapkan adalah 1 jam 30 menit 48 detik, hal tersebut berkurang dari hasil pengujian pra riset yang membutuhkan waktu 2 jam 26 menit 44 detik. dalam menyelesaikan tugas mencapai 88% dari target 80% dan efisiensi waktu keseluruhan mencapai 86% dari target 80%. sehingga dengan dibuatnya design system merupakan solusi yang tepat diimplementasikan di UMMI sukabumi.
SISTEM INFORMASI MANAJEMEN INVENTORI BAN MOBIL PADA PERUSAHAAN DISTRIBUTOR BAN IMPOR
Authors: Mardiyanto Tajri Nurwandi , Lubis Riani
Published: June 2022

Sebuah perusahaan distributor ban mobil yang berlokasi di daerah Bandung, menjadi objek penelitian ini. Perusahaan ini mengimport ban mobil berbagai ukuran dari negara Cina. Permasalahan yang terjadi di perusahaan tersbut diantaranya adalah sering terajadinya kelebihan muatan di gudang penyimpanan ban yang disebabkan oleh fluktuatifnya permintaan ban serta pengaturan dalam penyimpanan ban di gudang penyimpanan yang mempertimbangkan kapasitas maksimum dari gudang tersebut. Maka solusi yang dapat dilakukan untuk mengatasi permasalahan tersebut adalah membangun suatu sistem informasi manajemen inventori. Adapun perencaan pemesanan menggunakan Economic Order Quantity (EOQ), Proses keluar masuk barang menggunankan metode Pick To Order dengan mengaplikasikan metode First In First Out (FIFO) dan Last In First Out (LIFO), Dan Proses penyimpanan ban menggunakan metode Class Based Storange. Berdasarkan hasil pengujian sistem dapat disimpulkan bahwa sistem informasi manajemen inventori yang dibangun dapat mempermudah manajer operasional dalam menentukan jumlah ban yang akan dipesan setiap pemesanannya, mempermudah admin gudang saat proses keluar masuknya ban, mempermudah admin gudang dalam menentukan jumlah maksimal kapisatas gudang karena ban yang lebih tertata
PENERAPAN PROFILE MATCHING UNTUK PENILAIAN KINERJA KARYAWAN (STUDI KASUS : PT DELTA GARDA PERSADA)
Authors: Rosmania Nita , Kristiana Titin
Published: June 2022

Kinerja karyawan yang berkualitas menentukan perkembangan dan kemajuan suatu perusahaan untuk mencapai visi dan misinya. Dalam suatu perusahaan, karyawan merupakan asset yang harus dijaga dengan baik. Karyawan yang berkualitas adalah yang memiliki etos kerja yang tinggi, maka penting bagi setiap pimpinan untuk menentukan dan memilih karyawan terbaik untuk mendapatkan penghargaan atas kerja keras yang dihasilkan. Adanya suatu penghargaan terhadap karyawan dapat menciptakan semangat dan motivasi dengan kriteria yang ditentukan oleh masing-masing pimpinan. Proses penilaian kinerja karyawan pada PT Delta Garda Persada masih bersifat subyektif dan hanya berdasarkan faktor tertentu saja. Oleh karena itu Sistem Pendukung Keputusan (SPK) sangat membantu dalam hal pengambilan keputusan yang dibuat, kemudian dianalisis dengan menggunakan metode Profile Matching. Beberapa kriteria penilaian yang digunakan dalam Sistem Pendukung Keputusan penilaian kinerja karyawan di PT Delta Garda Persada yaitu Aspek Sikap Kerja, Aspek Kecerdasan, Aspek Kinerja. Maka dari itu pengambilan keputusan dengan metode Profile Matching akan menjadi lebih cepat, akurat, efektif dan efisien untuk membantu pimpinan menghasilkan keputusan yang terbaik.
Potencial educativo em Unidade de Conservação: o caso do Parque Natural Municipal de Governador Valadares (MG)
Authors: Geber Fernanda Morozesky , Souza Maria Celeste Reis Fernandes de , Campos Renata Bernardes Faria
Published: June 2022

Este estudo considera as dimensões natural, social e política de unidades de conservação, em que educação e proteção ambiental devem ocorrer de forma conjunta e integrada. Assim, refletimos sobre o potencial desses espaços especialmente protegidos, a partir da análise do plano de manejo, das atividades realizadas no Parque Natural Municipal de Governador Valadares e de caminhadas nas trilhas ali existentes. Entre nossos resultados, destacam-se o excelente potencial da estrutura física, a sobrecarga da equipe de funcionários, a subnotificação das atividades realizadas no parque, e suas possíveis implicações para que o parque seja considerado menos importante do que de fato é para o território educativo do município.
Jogos dramáticos como proposta de Educação Ambiental
Authors: Poletto Glenda Igridi , Rodrigues Andriane de Melo
Published: June 2022

A presente proposta educativa tem por finalidade trazer informação e consciência para jovens a respeito da atual crise ambiental e, mais especificamente, da perda de recursos ambientais que afetam o Cerrado Brasileiro, por meio de oficinas teatrais em um espaço não formal. O projeto utilizou as artes cênicas como mediadora do processo e como ferramenta para investigação das relações existentes entre arte e educação, promovendo debate e conscientização ambiental. Além da análise de documentário e de texto teatral, os integrantes participaram de jogos dramáticos, com o objetivo de promover conhecimento artístico e pensamento crítico acerca dos processos criativos para criar uma peça de teatro com temática socioambiental.
Educação Ambiental na gestão dos resíduos sólidos gerados por eventos culturais no Parque das Nações Indígenas, Campo Grande (MS)
Authors: Brito Ingryd Khristina de , Marques de Araújo Giselle , Matias Rosemary , Oliveira Ademir Kleber Morbeck de
Published: June 2022

O Parque das Nações Indígenas, parque urbano localizado na cidade de Campo Grande, é um espaço utilizado pela população e considerado um ponto turístico. Eventualmente sedia eventos culturais, como shows musicais. O objetivo deste trabalho é investigar as consequências da ausência da política de Educação Ambiental em relação a estes eventos, em especial a respeito da geração de resíduos sólidos. Foi realizado o levantamento de eventos ocorridos entre os anos de 2013 e 2019, verificando as exigências e monitoramento do poder público em relação aos resíduos produzidos, contidos em dados oficiais dos órgãos de gestão ambiental. Como resultado foi observado que não há uma gestão adequada dos resíduos, nos eventos ocorridos no parque e sugere-se que se adotem medidas de Educação Ambiental para adequado manejo de resíduos produzidos em parques urbanos, bem como sugere-se que sejam suspensos tais eventos até a construção de um planejamento adequado.
As cavernas como tema interdisciplinar no ensino fundamental
Authors: Menin Daniel , Tognetta Luciene Regina Paulino , Bacci Denise de La Corte
Published: June 2022

Frente às atuais discussões globais sobre a conservação da diversidade natural, este artigo tem como objetivo relatar experiências na implementação de um projeto educativo que aborda a Espeleologia como tema gerador e suas possibilidades de diálogo com o currículo escolar do ensino fundamental numa perspectiva interdisciplinar. O projeto educativo apoia-se nos referenciais teóricos da Geoconservação, da Educação Ambiental e da interdisciplinaridade, baseando-se na própria atividade e participação do sujeito como construtor dos conhecimentos para promover a aprendizagem. Desenvolvido em escolas públicas e particulares, para a faixa etária de 10 a 12 anos, o projeto é composto de duas fases, uma relacionada à formação continuada de professores, que aborda conceitos específicos de espeleologia, e outra na qual os estudantes desenvolvem projetos interdisciplinares relacionados ao tema das cavernas. Os resultados indicam que este projeto educativo sensibilizou os estudantes para a compreensão das diferentes áreas do conhecimento associada à espeleologia, promoveu aprendizagens significativas e contextualizadas e possibilitou o desenvolvimento de projetos interdisciplinares nas escolas, com participação efetiva dos estudantes.
Cultura para o desenvolvimento sustentável em Salvador (BA): um relato a partir do ensino de direito ambiental em uma universidade privada
Authors: Maia Guilherme Aparecido da Silva
Published: June 2022

O objetivo deste trabalho é apresentar resultados de três projetos de ensino e pesquisa, desenvolvidos pelo autor, quando docente da disciplina Direito Ambiental, no período de 2011 a 2017, do Centro Universitário Jorge Amado (UNIJORGE), Salvador, Bahia, Brasil. A metodologia adotada para consolidação do trabalho foi a pesquisa-ação (TRIP, 2005). Os resultados do trabalho foram percebidos em três momentos: Projeto “Cultura para o Desenvolvimento Sustentável”, realizado no Terreiro de Jesus, Pelourinho, Salvador, Bahia (i); Exposição didático-fotográfica semestral dos acadêmicos do curso de Direito, Universidade Jorge Amado, Salvador, Bahia (ii); e, Projeto “As águas da terra de Iemanjá” (iii), Salvador, Bahia, Brasil.
Socio-cultural competence in foreign language training of future aviation specialists
Authors: Radul Serhii , Kharlamova Liliia , Lomakina Maryna , Zelenska Liliia , Tymchenko Svitlana
Published: June 2022

Purpose is an empirical study of the content parameters of socio-cultural competence in foreign language training of future aviation specialists at the stage of their training in a higher educational institution. The research was conducted in the form of ascertaining experiment with a comparison of the content parameters formation. Valid standardized methods, purposeful observation, reliability coefficients are applied. It is established and proved that effective foreign language training of future aviation specialists promotes ethnic (first year of study M = 25.01; third year of study M = 31.56) and social tolerance (first year of study M = 28.12; third year of study M = 32.45). They are key ones in the formation of socio-cultural competence. Differences in the formation of key research parameters of socio-cultural competence of respondents of the first and third years of study (t=2.2–5.1; р<.05; р<.01) were designated. It is substantiated that foreign language training of future aviation specialists in the context of socio-cultural competence formation has axiological and praxeological orientations. It is generalized that the obtained scientific facts are essential. It is expedient to operationalize and implement them in the educational process of foreign language training of future aviation specialists.
Draft genome sequence of Bacillus thuringiensis strain S1287, an isolate showing insecticidal activity against coleoptera / Projeto de sequência do genoma de Bacillus thuringiensis strain S1287, um isolado mostrando actividade insecticida contra coleoptera
Authors: Queiroz Paulo , Togawa Roberto , Martins Érica , Grynberg Priscila , Monnerat Rose
Published: June 2022

Bacillus thuringiensis is an important bacterium showing insecticide proteins effective to control several important medical pests, including several Diptera species, like Diptera, Coleoptera and Lepidoptera. This work describes the draft genome sequence of B. thuringiensis S1287, which contains genes encoding the parasporal crystal cry, like Cry1Ab, Cry1Bb, Cry1C, Cry1D, Cry1Fb, Cry1Hb, Cry1Id, Cry1Ja, Cry1Ka and Cry1Nn. The genes belonging to the Vip Family were identified, Vpb1Aa, Vpa2Aa and Vip3Ag. Genes coding for plant growth promotion pathway were found.
Draft genome sequence of Bacillus thuringiensis strain 2193, an isolate toxic for lepidopteran / Projeto de sequência genómica de Bacillus thuringiensis strain 2193, um tóxico isolado para lepidopterano
Authors: Queiroz Paulo , Togawa Roberto , Grynberg Priscila , Martins Érica , Monnerat Rose
Published: June 2022

Bacillus thuringiensis is a Gram-positive bacterium which produces insecticidal proteins effective to control several medical and agriculture insect pests. These proteins belong to the Cry, Cyt, Vip and Sip families. This work describes the draft genome sequence of B. thuringiensis S2193, which contains genes encoding the parasporal crystal cry1Ad, cry1Ca, cry1Da, cry1Fa, cry1Ia, cry2A, cry9Ea and vip3Ca. Genes coding for plant growth promotion pathway were identified. And the operon which encodes the bacteriocin Thuricin 17 were annoted. We found the gene which encodes the toxin enhancin.
As políticas públicas educacionais no Brasil: a crise estrutural e seus efeitos sobre o direito ao ensino médio SEDUC-AM em 2019/2020 / Educational public policies in Brazil : the structural crisis and its effects on the right to high school SEDUC-AM in 2019/2020
Authors: Barbosa Wilka Monnah Cordeiro , Beranger Juan Alberto
Published: June 2022

As políticas públicas pensadas para melhorar a qualidade do ensino nem sempre se tornam realidade na sua prática. Isso se deve em parte a má versação de recursos e o descompromisso de gestores com a coisa pública. Apreender com as implicações da reforma do Ensino Médio pela Lei nº 13.415/2017, no Brasil e no estado do Amazonas, dentre os anos de 2017 a 2019 é uma maneira de contribuir com a verificação das melhorias. Neste estudo buscou-se verificar a relação das políticas educacionais e sua relação com a crise econômica estrutural que ultrapassam as fronteiras brasileiras e impactam a economia de todos os países, o que pode ter acarretado consequências sociais negativas sobre a garantia do direito à educação. Procurou-se identificar no estado do Amazonas os tipos de mudanças na forma, no conteúdo e na finalidade do Ensino Médio em comparação com o quadro brasileiro. Foi realizada a pesquisa quali- quanti com a organização de cinco etapas articuladas entre si, sendo: levantamento bibliográfico, pesquisa documental, entrevistas com pedagogos, análise dos dados e a proposta e/ou sugestão para organizar o trabalho pedagógico da escola. Procedendo ao levantamento bibliográfico buscou-se fundamentar o contexto econômico e das políticas e reformas voltadas para o Ensino Médio, desde a década de 1990, como financiamento, gestão, currículo, avaliação e o trabalho docente. A pesquisa documental foi realizada com o propósito de caracterizar e analisar as alterações que as propostas para organização do Ensino Médio promoveram referente ao currículo, à estruturação dos tempos de aula e à avaliação tanto em nível nacional quanto no Amazonas. Os documentos adquiridos abrangeram portarias, resoluções, medidas provisórias, decretos, leis e demais documentos que tem correlação com o Ensino Médio. A análise coube a interpretação das leituras, documentos e entrevistas com os sujeitos da pesquisa. Os resultados sugerem a composição de proposta de intervenção no sentido da importância do trabalho pedagógico que requer uma teoria que compreenda a escola e seus papéis, bem como a melhoria na organização da formação de professores e a avaliação sobre os resultados alcançados. Todo esse processo deve ser planejado com vistas à formação continuada dos professores e a garantia do direito de aprender a pensar criticamente dos adolescentes e jovens da escola.
Estruturas e representações sobre a desigualdade de gênero na gestão da água em Ouro Preto/ MG: uma análise da percepção de conselheiros e gestores dos órgãos municipais / Structures and representations on gender inequality in water management in Ouro Preto / MG: an analysis of the perception of councilors and managers of municipal bodies
Authors: Rosa Alexsandra Matilde Resende , Guarda Vera Lúcia de Miranda , Alves Kerley dos Santos
Published: June 2022

O artigo analisa a participação das mulheres na gestão das águas no município de Ouro Preto/MG, identificando se na percepção dos funcionários e conselheiros existem desigualdades de gênero nas estruturas organizacionais dos órgãos e conselhos em que atuam e se os entrevistados reconhecem diferenças relativas a papéis de gênero na esfera pública e privada. Em relação à classificação metodológica se trata de uma pesquisa aplicada, quantitativa, descritiva e de levantamento. A coleta de dados foi através da aplicação de questionários, que foram respondidos por 48 conselheiros e 72 funcionários dos órgãos responsáveis pela gestão da água no município. Os órgãos e conselhos que participaram do estudo foram: SEMAE, COMUSA, CODEMA, Secretária Municipal de Meio Ambiente e Subcomitê de Bacia Hidrográfica - Nascentes. Conclui-se que em relação à percepção dos gestores existem desigualdades de gênero nas estruturas organizacionais e nos papéis sociais de homens e mulheres na sociedade.
Análise estrutural e dimensionamento de linhas de transmissão de energia / Structural analysis and dimensioning of power transmission lines
Authors: Inoue Hisashi , Torres Fabrício Cotta
Published: June 2022

O trabalho se baseia na análise estrutural e dimensionamento de uma linha de transmissão de energia (LT) autoportante, sendo uma estrutura de suspensão de circuito simples, com tensão de 230 kV, altura de 24 metros e vão entre as torres de 500 metros.A torre será em sistema estrutural treliçado, formada por perfis de cantoneiras de abas iguais em aço ASTM A36.
Letramento em saúde na orientação às gestantes em pandemia do novo coronavírus: relato de experiência / Health literacy in advice to pregnant women in the novo coronavírus pandemic: experience report
Authors: Coimbra Marli Aparecida Reis , Fiomari Kelly Karina , Souza Mirna Nunes da Silveira , Lopes Fabiana Augusta Moreira , Ferreira Lúcia Aparecida
Published: June 2022

Objetivo: O estudo teve o objetivo de relatar as inquietações de participantes de um programa de orientação à gestante na perspectiva de uma equipe multiprofissional, no período de pandemia do novo coronavírus (SARS-Cov2), segundo o princípio do letramento em saúde. Método: Trata-se de um relato de experiência de um programa de orientações às trabalhadoras gestantes, intitulado Programa “GeraVida”, em uma universidade pública federal, no interior de Minas Gerais, Brasil, de maio a outubro de 2020. Resultados: As discussões dos encontros foram organizadas em duas categorias: as expectativas sobre a amamentação e os fatores que impactam o período gestacional. Participaram 39 funcionárias. Devido ao período de pandemia, o evento foi organizado pela primeira vez na modalidade remota. Conclusão: Mesmo neste formato foi possível a aproximação, sanar dúvidas, perceber a compreensão e envolvimento do grupo. O letramento em saúde adequado é um caminho para o empoderamento nos processos de saúde.
Revisão crítica da avaliação neuropsicológica do transtorno do espectro autista / Critical review of the neuropsychological assessment of autism spectrum disorder
Authors: Junior Estácio Amaro da Silva , Câmara Ítalo de Melo , Torres Matheus Coelho , Costa Ian Félix Martins , Da Silva Tiago César Costa , Sales Erich Barbosa Albuquerque , De Queiroz Andressa Gabriella Duarte , Dantas José Lucas Formiga
Published: June 2022

A avaliação neuropsicológica do Transtorno do Espectro Autista (TEA) consiste em uma investigação criteriosa de alterações e competências cognitivas e comportamentais, que, através de uma abordagem individualizada, permite o delineamento de importantes estratégias de intervenção. Devido à complexidade e heterogeneidade do perfil neuropsicológico do TEA, especialmente das funções executivas (FE), este estudo se propõe, por meio de uma revisão crítica da literatura, sintetizar e refletir sobre os achados atuais, identificando relações, contradições, lacunas e sugerir novos rumos para estudos futuros. Como resultado, notam-se prejuízos sobre os domínios da teoria da mente, habilidades sociais e comunicação; memória de longo prazo preservada; comprometimento geral sobre as FE examinadas; e prejuízos significativos sobre flexibilidade, generatividade e memória de trabalho, mesmo sem TDAH comórbido e nível intelectual comparável. Realizar previsões de um perfil diferencial em subdomínios das FE ainda tem sido um grande desafio e é essencial realizar uma análise crítica, com base no que se encontra, na literatura científica, sobre o tema.