bluuebunny/crossref_metadata_2025 huggingface dataset:
Study on the influence of keratinized mucosa on the evolution of non-surgical peri-implant treatment in implants with peri-implantitis
Authors: Fons-Badal Carla , Panadero Rubén Agustín , Labaig-Rueda Carlos , Solá-Ruiz Maria Fernanda , García-Selva Marina , Font Antonio Fons
Published: May 2022

The main objective of this study was to evaluate the response to treatment in implants with peri-implantitis depending on the presence or absence of keratinized mucosa. Materials and Methods: 100 implants with peri-implantitis were treated non-surgically at the Prosthodontics and Occlusion Teaching Unit of the University of Valencia. Records were registered at day zero (recession, bleeding on probing, suppuration, probing depth, bone loss and plaque index), at 1, 3 and 6 months. Results: In both groups a slight increase in recession and disappearance of suppuration took place, and no bone loss was observed during the 6 months. However, both probing depth, bleeding on probing and plaque index, after an initial reduction, increased again in the group without keratinized mucosa (KM). Conclusions: Implants with KM presented better results after non-surgical treatment as well as in their medium-term evolution, although it would be advisable to increase the number of samples in order to achieve greater scientific evidence and standardization in the treatment protocol. Clinical relevance: The role of keratinized mucosa in the development of peri-implantitis has been mentioned in many publications, but less has been emphasized regarding its influence on the success of the treatment of this pathology. The presence of keratinized mucosa has been found to play a determinant role in the evolution of non-surgical treatment of peri-implantitis. The amount of keratinized mucosa should be considered in the treatment of peri-implantitis, as well as when planning connective tissue grafts accompanying the decontamination of implants in the absence of keratinized mucosa.
The association between genetic variation rs2292832 and the processing efficiency of pre-miR-149 affects the risk of breast cancer
Authors: Fakhrezare Farzaneh , Ebrahimi Seyed Omar , Reiisi Somayeh
Published: May 2022

Background: microRNAs (miRNAs) play key roles in regulating cancer development, including breast cancer. Variation in miRNA genes can associate with the risk of cancer by alterations in the miRNA’s processing and maturation. Therefore, human blood samples and breast cancer cell line (MCF7) were analyzed to study any possible association between the genetic variant (rs2292832) in the miR-149 precursor and breast cancer susceptibility. Methods: To study the role of rs2292832 polymorphism in breast cancer, the miR-149 gene variant was genotyped using PCR-RFLP. For evaluating the effect of SNP on function and expression levels of mature miR-149, we inserted pre-miR-149 and flanking region with CC or TT genotype into a pEGFPN1 expression vector, and qPCR was accomplished. Cell survival, proliferation, and migration properties investigated by MTT and wound healing assay. Statistical analysis was carried out for data analysis.Results: T allele in variant rs2292832 is associated with an increased risk of breast cancer. Such association was also obtained in co-dominant (OR=2.37) and dominant (OR=1.90) models. The variant allele led to reduced production of mature miR-149 and resulted in increased cell proliferation and migration of MCF7 cells. Conclusion: These findings suggest that miR-149 suppresses tumor cell proliferation, and the pre-mir-149 polymorphism affects the processing of miR-149, causing an alteration in the abundance of the miRNA mature form, which can regulate tumor progression and metastasis.
Urban Ecology and Biological Studies in Brazilian cities: a systematic review
Authors: Sartori Richieri Antonio , Gomes Abraão Carvalho , Narcizo Amanda , Mata Stella , Cárcamo Anna Thereza Bezerra Mello , Matos Rayanne Moreira Andrade , Correa Angelo Antonio , Santana Mariana Henriques , Vieira Maura Andrade , Rajão Henrique
Published: May 2022

The number of papers focusing on ecological interactions in urban environments has increased in recent years. This review aimed to address the panorama of urban ecology and biological surveys in Brazil. A systematic search was carried out using the Web of Science and Scopus platforms for papers on urban ecology to understand which institutions, authors, themes, cities, biomes, states, and regions have addressed the theme in Brazil to date. A total of 932 articles were found, in 196 scientific journals. Most papers were published between 2010 and 2019. This involved 350 municipalities in the five Brazilian regions, with Curitiba, São Paulo, and Rio de Janeiro being the municipalities with the most papers. São Paulo was the state that presented the most papers, with 23.7% of the total, and the Southeast region was the most representative with 36.6%. The biome with the highest concentration of papers (61.2%) was the Atlantic Forest. A total of 2537 authors were registered, affiliated with a total of 413 institutions from 19 countries. The institutions with the most papers were the Federal University of Paraná and University of São Paulo. The most discussed topic was related to botany (69%), and the most used keyword was “urban afforestation”. The number of papers published was greater in municipalities with higher human development index, number of inhabitants and relative urbanized area. This review revealed the scarcity of studies in low-income areas, and the need for greater incorporation of the social aspect, landscape ecology and ecological interactions in urban ecology.
Subaqueous bioprinting: a novel strategy for fetal membrane repair with 7-axis robot-assisted minimally invasive surgery
Authors: Zhao Wenxiang , Hu Chuxiong , Xu Tao , Lin Shize , Wang Ze , Zhu Yu
Published: May 2022

Premature rupture of membranes (PROM), defined as breakage of the amniotic sac prior to delivery, complicates 2–4% of pregnancies, leads to one-third of preterm births, and increases the risk of perinatal mortality and morbidity in survivors. In vivo bioprinting enables the fabrication of three-dimensional (3D) structures directly for tissue repair or reconstruction in living animals but is challenging to perform in the subaqueous narrow space where fetal membranes (FMs) are located. This study reports a novel strategy consisting of an ultrafast photoresponsive hydrogel (1.5 s) and a 7-axis bioprinting robot to perform subaqueous in vivo bioprinting in a minimally invasive approach. Specially, a hydrogel patch with designed gel rivets is printed subaqueously for the therapeutic needs of PROM, and the patch displays robust tissue adhesion, favorable biocompatibility, mechanical properties resembling native tissues, and an appropriate sealing timescale to prolong pregnancy. Experiments performed in an in vitro uterus model and a mid-gestational rabbit model show a favorable sealing effect for PROM, which validates the potential of our subaqueous bioprinting approach for application. This study not only expands upon in vivo bioprinting techniques but also provides valuable insights for the treatment of PROM and other diseases involving tissue injury in clinical settings.
Multi-target Location Based on Compressed Sensing Direct Position Determination with Missing Information
Authors: Huang Xiangsong , Yuan Jia , Yang Zhigang , Su Runjia
Published: May 2022

It often happens that we cannot acquire all information available to locate targets as obstacles exist between observation stations and targets or there is something wrong with some observation stations. Both cases are called the circumstance of missing information where partial information can be observed, resulting in poor positioning performance of multi-target location. Considering the sparse characteristic and random subsampling of Compressed Sensing (CS) theory, we think of a possibility of applying Compressed Sensing Direct Position Determination (CSDPD) to lessen the impact of missing information on multi-target location. Orthogonal matching pursuit (OMP) algorithm is a kind of greedy algorithm of CS, which can be utilized to lessen the impact of missing information. For better positioning accuracy, an improved OMP algorithm based on DPD (IOMP-DPD) is proposed through a new method of updating index set to lessen the possibility of incomplete location. It confirms by simulations that the improved method yields more accurate positioning results than OMP algorithm and outperforms than the existed MUSIC algorithm based on DPD (MUSIC-DPD), revealed in estimating more target locations in the same positioning conditions and smaller positioning errors when locating the same targets.
Incidental learning of faces during threat: No evidence for increased autonomic arousal to “unrecognized” threat identities
Authors: Schellhaas Sabine , Schmahl Christian , Bublatzky Florian
Published: May 2022

Remembering an unfamiliar person and the contextual conditions of that encounter is important for adaptive future behavior, especially in a potentially dangerous situation. Initiating defensive behavior in the presence of former dangerous circumstances can be crucial. Recent studies showed selective electrocortical processing of faces that were previously seen in a threat context compared to a safety context, however, this was not reflected in conscious recognition performance. Here, we investigated whether previously seen threat-faces, that could not be remembered, were capable to activate defensive psychophysiological response systems. During an encoding phase, 50 participants with low to moderate levels of anxiety (partially inpatients) viewed 40 face pictures with neutral expressions (6s each), without an explicit learning instruction (incidental learning task). Each half of the faces were presented with contextual background colors that signaled either threat-of-shock or safety. In the recognition phase, all old and additional new faces (total of 60) were presented intermixed without context information. Participants had to decide whether a face was new or had previously been presented in a threatening or a safe context. Results show moderate face recognition independent of context conditions. Startle reflex and skin conductance response (SCR) were more pronounced for threat compared to safety during encoding. For SCR, this differentiation was enhanced with higher levels of depression and anxiety. There were no differential startle reflex or SCR effects during recognition. From a clinical perspective, these findings do not support the notion that perceptual biases and physiological arousal directly relate to threat-associated identity recognition deficits in healthy and clinical participants with anxiety and trauma-related disorders.
Rapid Vertical Exchange at Fronts in the Northern Gulf of Mexico
Authors: Qu Lixin , Thomas Leif , Wienkers Aaron , Hetland Robert , Kobashi Daijiro , Taylor John , Hsu Fucent , MacKinnon Jennifer , Shearman R. , Nash Jonathan
Published: May 2022

The Texas-Louisiana Shelf in the Northern Gulf of Mexico is home to the second largest human-caused dead zone in the world. Here, the nutrient-laden, stratified waters of the Mississippi River plume condition hypoxia in a bottom layer during the summer. The plume also generates strong fronts, features of the circulation that are known pathways for the exchange of water between the ocean surface and the deep. Using a combination of high-resolution observations and numerical simulations, we show that the vertical exchange at these fronts can be quite rapid and can lead to the oxygenation of bottom water when the fronts are forced by the summer land-sea breeze. These winds generate strong inertial oscillations, which set up a diurnally-pulsing vertical circulation at the fronts that draws bottom waters up to the surface mixed layer. The simulations suggest that during these “breaths” the rate of oxygenation of the bottom waters is comparable to deoxygenation by the respiration of organic matter over the shelf and hence could play an important role in the evolution of the region’s dead zone.
Identification of genes involved in the effects of hypoxia-inducible factor-2 on articular chondrocytes using bioinformatic analysis and validation in osteoarthritis
Authors: Zheng Jiaxuan , Guo Xiangjun , Zhou Kai , Cao Huiyuan , Fang Yehan , Shi Li , Huang Hui
Published: May 2022

Although over-expression of hypoxia-inducible factor-2 alpha (HIF-2α) can result in cartilage destruction and osteoarthritis (OA) development, the underlying mechanisms remain poorly understood. Here, we investigated the molecular mechanisms in chondrocytes over-expressing HIF-2α. The GeneCloud of Biotechnology Information platform was used to identify differentially expressed genes (DEGs). Using the GEO GSE104794 dataset of control (empty adenovirus, n = 4) and experimental (recombinant adenovirus expressing HIF-2α, n = 4) groups, we performed DEG, Gene Ontology, pathway, pathway network, and gene signal network analyses. Similarly, DEG analysis was performed for the GEO GSE51588 dataset of control (non-OA, n = 4) and experimental (OA, n = 20) groups. Thereafter, intersection of GSE104794 gene signal network analysis and GSE51588 DEG analysis was performed for the key genes, validated by quantitative reverse transcription-polymerase chain reaction. A total of 542 DEGs were identified, among which, the 10 most significant genes in the gene signal network were Nfkb1, Tlr2, Nt5e, Enpp1, Entpd3, Vegfa, Ptgs2, Socs3, Fos, and Epas1. The key genes in OA were LUM, ENTPD3, SMPD3, FGFR3, GPX3, IRAK3, EREG, HTR2A, TLR2, and CDA. Taken together, we screened key genes that are potentially involved in osteoarthritis, thereby providing a basis for identifying valuable markers for this disease.
Task prioritization modulates alpha, theta and beta EEG dynamics reflecting proactive cognitive control
Authors: Liegel Nathalie , Schneider Daniel , Wascher Edmund , Arnau Stefan
Published: May 2022

Most neuroscientific studies investigating mental effort apply unspecific effort allocation paradigms. In contrast, the present EEG study targets specific effort allocation during task prioritization. Twenty-eight participants performed a cued number classification task during the retention interval of a working memory task including retrospective cues. One of two possible number classifications was done per trial. Each trial started with a cue indicating which of the two tasks would be more important in the upcoming trial. Subjects were told to engage in both tasks, but to concentrate on the important one. Feedback given at the end of each trial was calculated based on task performance, with scores obtained from the relevant task being tripled.Participants performed significantly better in either task when it was important compared to when not. Task prioritization modulates theta, alpha and beta oscillations, predominantly during task preparation. Multivariate pattern analysis revealed that the exact type of the two possible number classifications was decodable, however, decoding accuracy did not depend on task importance. Hemispheric alpha power asymmetries indicating attentional orienting between working memory representations also did not depend on task importance. The findings suggest that task prioritization primarily affects proactive cognitive control on a superordinate level.
Ethanol Intoxication-Related Metabolic and Biochemical Disturbances in Adolescents: A Matched Case- Control Study
Authors: Hanalioglu Damla , Ozkocer Cansu , Ozalp Esra Can , Dikmen Zeliha Gunnur , Pinar Asli , Teksam Ozlem
Published: May 2022

Background: Alcohol is the most widely consumed substance by adolescents. However, only a few studies investigated metabolic and biochemical changes related with acute ethanol intoxication (AEI) with conflicting results. In this case-matched study, we aimed to investigate clinical and laboratory characteristics of the adolescents with AEI presented to pediatric emergency department (ED) at a tertiary referral center. Methods: All consecutive adolescents with AEI (serum ethanol level >50 mg/dL) (n=335) and their sex- and age-matched control subjects with undetectable ethanol levels who presented to the pediatric ED between 2006-2019 were included in this case-matched study (a total of 670 patients). Demographic, clinical and laboratory data was collected through electronic health records retrospectively. Results: Mean age was 16.0±1.5 years and M/F ratio was 1.5. Mean serum ethanol level was 156.4±58.4 mg/dl (range 50.8-341.2 mg/dl) in the AEI group. Glasgow coma scores were lower in the AEI group (14 [14-15] vs. 15 [15-15], p<0.001). Laboratory investigations revealed that acidosis (16.3%), hyperlactatemia (60.9%), hypoglycemia (1.7%), hypernatremia (2.2%), hypokalemia (12.3%), hyperchloremia (20.4%), hypocalcemia (13.9%), hypermagnesemia (9.7%), hyperalbuminemia (10.4%) were significantly more common in the AEI group as compared to the control group. Blood pH, lactate, Na+, K+, Ca++, Mg++, albumin, BUN and uric acid levels were correlated with serum ethanol levels. Conclusion: Being one of the largest case-matched study of AEI among adolescents published in the literature, this study shows that acute ethanol intoxication frequently leads to mild to moderate metabolic/biochemical derangements in adolescents. Clinicians should be aware of these findings in the management of AEI.
A Study on CBD and Δ9-THC Analysis and Telemedicine of Korean Hemp Seed Foods Manufactured by Oriental Medicine Method
Authors: Kim Namsoo Peter , Eo Yoonhyeong , Aditya Abhilash , Kim Jihye , Kwon SoonHyup
Published: May 2022

Background

Despite the UN's recommendation to abolish restrictions on Cannabidiol (CBD) in the medical and recreation sectors, detailed regulations worldwide on food-grade CBD & Tetrahydrocannabinol (THC) are not adequately reflected.

Methods

The methods of extracting hemp, which was practiced for thousands of years as detailed in ancient oriental medicine literature, were compared with recent Western cannabis case studies, and the possibility of delivering the extracts through food via telemedicine was evaluated. After decades of research, attempts have been made to use improvised hemp or cannabis for medical and cosmetic purposes with lower CBD and THC content, but micro-components, including CBD and THC analysis of Korean hemp seeds(Cheong-Sam), have yet to be accurately reported.

Results

The CBD and THC contents of Korean hemp seeds were measured within the HPLC 300 measurement range and analyzed by diluting 10 times or more to imitate the projected concentration in food. The CBD/THC content of Korean hemp seeds produced in the Andong Hemp Special Zone (AHSZ) was extracted by either hot water extraction or cold compression, similar to traditional medical literature, and then analyzed thoroughly to examine the applicability of traditional oriental methods to food.

Conclusion

CBD and THC contents were safe at less than 3 ppm when using Korean hemp. In the method extracted from the literature, CBD, Δ 9 -THC, tetrahydrocannabinolic acid (THCA), Cannabidiolic acid (CBDA), Cannabidivarinic acid(CBDVA), Cannabigerolic acid (CBGA), Tetrahydrocannabivarin acid (THCVA) responding to human reaction mechanisms were not detected in foods containing Korean hemp (Cheong-Sam) seed and oil used in this study.
Computational Integrated Smart HFRTB Concrete for Sustainable Constructions
Authors: Bajad Mohankumar
Published: May 2022

When hybrid fibre Reinforced Ternary Blended (HFRTB) concretes were exposed to sustained elevated temperatures for three hours (SET-3hrs), the effect of two cooling regimes (sudden and gradual) was examined. At 100o C intervals, the temperature ranges from 100o C to 1000o C. Traditional concrete is compared to two ternary blended (TB) concretes (Cement + fly ash + GGBFS and Cement + fly ash + silica fume), as well as hybrid fibres (Galvanized iron + polypropylene). The specimens were put through a series of strength and durability tests, with slow cooling outperforming abrupt chilling.
Integrated Computational Materials Engineering With Reliability of Green Concrete (GC) For Environmental Safety Buildings
Authors: Bajad Mohankumar N.
Published: May 2022

The compelling usage of left-over constituents of warm force stations, for example, fly debris and bottom ash (BA) as halfway substitution to cement and fine aggregate (FA) in concrete diminish transfer issues. In this examination, the BA is exploited to supplant the normal stream sand up to 100% and in this manner, it diminishes the practice of waterway sand and reuse of BA in concrete which is eco-friendly and can be called green concrete (GC). The most significant overseeing factor that decides the oldness of the concrete structure is durability. BA is utilized as FA (30%, 60% and 100%) in concrete to lessen its ecological contamination (air, land, and water) and to ration characteristic waterway sand which is misused for development. GC comprising BA is intended for 30 MPa with fixed water to fastener proportion and slump esteem; and is assessed for, compressive strength, and elastic modulus. elongated term drying shrinkage (DS) was assessed for 365 days and an experimental relationship was created to foresee 10 years of DS of GC. Test outcome shows that 30%BA came about high compressive strength and elastic modulus than control blend at 90 days. The DS property of BA came about better and even prevalent execution for long term durability. The test examination additionally infers that GC comprising 30% of BA for FA substitution beats control concrete for the structured strength of 30 MPa at 90 days and anticipated DS for extended term durability provided that 10 years.
Toxic Chemical Pollutants in the U.S. States
Authors: Kagawa Shigemi , Tokito Shohei , Fujii Hidemichi , Lenzen Manfred , Faturay Futu , Okamoto Shunsuke
Published: May 2022

Footprint indicators are used to evaluate chemical substance management. However, determining the impact of chemical restrictions on manufacturing processes and supply chains without a footprint analysis of the entire lifecycle is difficult. Here, we propose a new framework for estimating chemical toxicity footprints utilizing the risk-screening environmental indicators (RSEIs) published by the U.S. Environmental Protection Agency. We conducted an empirical analysis using multi-states input–output data from the U.S. in 2017 to demonstrate the usefulness of the framework, and made policy recommendations based on the obtained results. According to the production-based RSEI scores, Texas, Pennsylvania, Illinois, and Louisiana accounted for 43% of the total human health risk in the U.S. By contrast, California and New York ranked first and second in consumption-based RSEI score (i.e., health risk footprint), respectively; however, the District of Columbia and Alabama ranked first and second in per capita footprint. Three significant risk transfers, accounting for 6% of the total in the U.S. economy, were found: from California to Texas, from New York to Texas, and from Florida to Texas. In conclusion, specific states such as Texas and Louisiana were at considerable risk from major chemical substance emissions triggered by final demands in mega cities such as New York and California. Thus, the federal and state governments should select high priority sectors and states according to the production-based RSEI scores. Relevant taxes can be collected from states based on the consumer’s responsibility. Footprint-based inter-state financial cooperation is crucial in mitigating chemical pollutions in the U.S.
Associations between Symptoms with Healthcare Utilization and Death in Advanced Cancer Patients
Authors: Farrell Megan M. , Jiang Cherry , Moss Gabriel , Daly Barbara J. , Weinstein Elizabeth , Kemmann Matthew , Gupta Mona , Lee Richard
Published: May 2022

Introduction: There is limited data about assessments that are associated with increased utilization of medical services among advanced oncology patients (AOP). We aimed to identify factors related to healthcare utilization and death in AOP. Methods: AOP at a comprehensive cancer center were enrolled in a Centers for Medicare & Medicaid Innovation program. Participants completed the Edmonton Symptom Assessment Scale (ESAS) and the Functional Assessment of Cancer Therapy – General (FACT-G) scale. We examined factors associated with palliative care (PC), acute care (AC), emergency room (ER), hospital admissions (HA), and death. Results: In all, 817 AOP were included in these analyses with a median age of 69. They were generally female (58.7%), white (61.4%), stage IV (51.6%), and represented common cancers (31.5% GI, 25.2% thoracic, 14.3% gynecologic). ESAS pain, anxiety, and total score were related to more PC visits (B = 0.31, 95% CI [0.21, 0.40], p < 0.001; B = 0.24 [0.12, 0.36], p < 0.001; and B = 0.038 [0.02, 0.06], p = 0.001, respectively). Total FACT-G score and physical subscale were related to total PC visits (B=-0.021 [-0.037, -0.006], p = 0.008 and B=-0.181 [-0.246, -0.117], p < 0.001, respectively). Lower FACT-G social subscale scores were related to more AC visits (B=-0.03 [-0.53, -0.004], p = 0.024); while, increased tiredness was associated with fewer AC visits (B=-0.039 [-0.073, -0.006], p = 0.023). Higher total ESAS scores were related to death within 30 days (OR = 0.87 [0.76, 0.98], p = 0.027). Conclusions: The ESAS and FACT-G assessments were linked to PC and AC visits, and death. These assessments may be useful for identifying AOPs that would benefit from routine PC.
Ti-based robust MOFs in the combined photocatalytic degradation of emerging organic contaminants
Authors: Rojas Sara , García-González Jessica , Salcedo-Abraira Pablo , Rincón Irene , Castells-Gil Javier , Padial Natalia M. , Marti-Gastaldo Carlos , Horcajada Patricia
Published: May 2022

Photocatalysis process is a promising technology for environmental remediation. In the continuous search of new heterogeneous photocatalysts, metal-organic frameworks (MOFs) have recently emerged as a new type of photoactive materials for water remediation. Particularly, titanium-based MOFs (Ti-MOFs) are considered one of the most appealing subclass of MOFs due to their promising optoelectronic and photocatalytic properties, high chemical stability, and unique structural features. However, considering the limited information of the reported studies, it is a hard task to determine if real-world water treatment is attainable using Ti-MOF photocatalysts. In this paper, via a screening with several Ti-MOFs, we originally selected and described the potential of a Ti-MOF in the photodegradation of a mixture of relevant Emerging Organic Contaminants (EOCs) in real water. Initially, two challenging drugs (i.e., the β-blocker atenolol (At) and the veterinary antibiotic sulfamethazine (SMT)) and four water stable and photoactive Ti-MOF structures have been rationally selected. From this initial screening, the mesoporous Ti-trimesate MIL-100(Ti) was chosen as the most promising photocatalyst, with higher At or SMT individual photodegradation (100% of At or SMT photodegradation in 2 and 4 h, respectively). Importantly, the safety of the formed by-products from the At and SMT photodegradation was confirmed. Finally, the At and SMT photodegradation capacity of MIL-100(Ti) was confirmed under realistic conditions, by using a mixture of contaminants in tap drinking water (100% of At and SMT photodegradation in 4 h), proven in addition its potential recyclability, which reinforces the potential of MIL-100(Ti) in water remediation.
Clinical Impact of CD200 on Outcome of Acute Myeloid Leukemia: A Cohort Study from a Single Center
Authors: Zhang Yanfang , Xi Quan , Song Guancheng , Song Ying , Lou Shifeng , Zhou Kang , Shen Yan , Deng Jianchuan
Published: May 2022

Purpose CD200, a trans-membrane protein belonging to the immunoglobulin superfamily, has been associated with a poor prognosis in acute myeloid leukemia(AML). We aimed to analyze the correlations between CD200 expression and prognostic significance in AML patients.Methods We retrospectively reviewed 160 AML patients evaluating the impact of CD200 expression on complete remission (CR), disease free survival(DFS), and overall survival (OS) in our institution between 2018 and 2021. Results CD200 were expressed in 100/160 (62.5%) cases, which were defined as the CD200 positive (CD200+) group and the rest of 60 cases were identified as CD200 negative(CD200-) group. A higher incidence of CD34 expression (P < 0.0001), NPM1 mutated(P=0.001) and unfavorable cytogenetic/molecular (P <0.0001) cases in CD200+ group. More intermediate cytogenetic/molecular status(P<0.0001) in CD200-group. Complete remission (CR) was evaluable achieved in70 patients (43.75%):34/100 (34%) in CD200+ and 36/60 (60%) in CD200- group (P = 0.001). CD200+ patients had significant lower probability to attain complete remission after first induction chemotherapy, both in univariate (P = 0.002) and multivariate (P = 0.023) analysis. In the whole population, CD200 expression had no significant effect on DFS (P = 0.837) and OS (P =0.155). In subgroup analysis we demonstrated that CD200 has obvious negative impact on OS in less-intensive approaches (P = 0.006) and in chemotherapy-only treatment (p=0.032) patients.Conclusion Expression of CD200 in AML contributes to low-remission rate and with a further worsening in long-term survival in patients receiving less-intensive approaches and chemotherapy-only treatment. CD200 positive is an indicator for low remission and poor prognosis in subgroups.
Total impact of oxidative stress genes on cardiovascular events – 7-year follow-up study
Authors: Racis Milena Rozalia , Stanisławska-Sachadyn Anna , Sobiczewski Wojciech , Wirtwein Marcin , Krzemiński Michał , Rynkiewicz Andrzej , Wasąg Bartosz , Jaguszewski Miłosz , Gruchała Marcin
Published: May 2022

Cardiovascular (CV) events are the number one cause of lifetime disability and deaths worldwide. It is well known that traditional risk factors do not fully correlate with clinical outcomes; therefore, searching for other markers that would explain CV events occurrence seems essential. Of importance, one of the main factors at the origin of CV events is oxidative stress, causing inflammation and atherosclerotic plaque instability. Therefore the present study was conducted to evaluate eight carefully selected genetic polymorphisms related to oxidative stress as risk modifiers for CV events. A cohort of 1020 patients with coronary atherosclerosis was analysed in a 7-year follow-up observational study. The following endpoints were assessed: CV death, myocardial infarction (MI) and a combined endpoint of CV death/MI/stroke. Our results show that single polymorphisms are not significant cardiovascular disease risk factors, but genetic risk score (GRS), defined as the accumulation of our eight studied polymorphisms, was significantly associated with the three studied follow-up endpoints. In conclusion, when regarding CV events, GRS investigated here can become clinically meaningful and undoubtedly adds to the knowledge in stratifying the risk of CV events.
Incidence, risk factors and prognosis of acute kidney injury in patients treated with immune checkpoint inhibitors: A retrospective study
Authors: Ji Ming-Su , Wu Rilige , Feng Zhe , Wang Yuan-Da , Wang Yong , Zhang Li , Sun Xue-Feng , Chen Xiang-Mei , He Kun-Lun , Cai Guang-Yan
Published: May 2022

BackgroundImmune checkpoint inhibitors (ICIs) change the prognosis of many cancer patients. With the increasing use of ICIs, immune-related adverse events (irAEs) are occurring, including acute kidney injury (AKI). This study aimed to assess the incidence of ICI-induced AKI and its risk factors and impact on mortality.MethodPatients treated with ICIs at the First Medical Center of the Chinese PLA General Hospital from January 1, 2014, to December 30, 2019, were consecutively enrolled, and risk factors affecting AKI development in patients treated with ICIs were analyzed using univariate and multivariate logistic regression. Medical record surveys and telephone inquiry were used for follow-up, and Kaplan–Meier survival analysis and Cox regression were used to analyze independent risk factors for death.ResultsAmong 1615 patients, 114 (7.1%) had AKI. Multivariate logistic regression analysis showed that anemia, Alb < 30 g/L, antibiotic use, diuretic use, NSAID use and proton pump inhibitor use were independent risk factors for AKI development in patients treated with ICIs. Stage 2 or 3 AKI was an independent risk factor for nonrecovery of renal function after AKI onset. Multivariate Cox regression analysis showed that anemia, Alb < 30 g/L, AKI occurrence, and diuretic use were independent risk factors for death in patients treated with ICIs, while higher baseline BMI, other tumor types, ACEI/ARB use, and chemotherapy use were protective factors for patient death.ConclusionAKI occurs in 7.1% of patients treated with ICIs. Anemia, Alb < 30 g/L, and combined medications are independent risk factors for AKI in patients treated with ICIs. Anemia, Alb < 30 g/L, AKI occurrence, and diuretic use were independent risk factors for death in patients treated with ICIs.
SDNN-PPI: Self-attention with deep neural networks effect on protein-protein interaction prediction
Authors: Li Xue , Han Peifu , Wang Gan , Chen Wenqi , Wang Shuang , Song Tao
Published: May 2022

Background: Protein-protein interactions (PPIs) dominate intracellular molecules to perform a series of tasks such as transcriptional regulation, information transduction, and drug signalling. The traditional wet experiment method to obtain PPIs information is costly and time-consuming. Result: In this paper, SDNN-PPI, a PPI prediction method based on self-attention and deep learning is proposed. The method adopts amino acid composition (AAC), conjoint triad (CT) and auto covariance (AC) to extract global and local features of protein sequences, and leverages self-attention to enhance DNN feature extraction to more effectively accomplish the prediction of PPIs. In order to verify the generalization ability of SDNN-PPI, a 5-fold cross-validation on the intraspecific interactions dataset of Saccharomyces cerevisiae (core subset) and human is used to measure our model in which the accuracy rates reach to 95.4% and 98.94% respectively. The accuracy rates of 93.15% and 88.33% are obtained in the interspecific interactions dataset of human-Bacillus Anthracis and Human-Yersinia pestis, respectively. In the independent data set Caenorhabditis elegans, Escherichia coli, Homo sapiens and Mus musculus, the prediction accuracy is 100% respectively, which is higher than the previous PPIs prediction methods. To further evaluate the advantages and disadvantages of the model, the one-core and crossover network are conducted to predict PPIs, and the data show that the model correctly predicts the interaction pairs in the network. Conclusion: In this paper, AAC, CT and AC methods are used to encode the sequence, and SDNN-PPI method is proposed to predict PPIs based on self-attention deep learning neural network. Satisfactory results are obtained on interspecific and intraspecific data sets, and good performance is also achieved in cross-species prediction. It can also correctly predict the protein interaction of cell and tumor information contained in one-core network and crossover network.The SDNN-PPI proposed in this paper not only explores the mechanism of protein-protein interaction, but also provides new ideas for drug design and disease prevention.
Network Pharmacology and Bioinformatics Identify the Potential Mechanism Underlying the Use of Hedyotis diffusa Willd for Treatment of Rheumatoid Arthritis
Authors: Deng Hui , Jiang Jing , Wu Lijuan , Zhang Qinglian , Sun Wenkui
Published: May 2022

Rheumatoid arthritis (RA) is a chronic, systemic, autoimmune disease that may lead to joint damage, deformity, and disability, if not treated effectively. Hedyotis diffusa Willd (HDW) and its main components have been widely used to treat a variety of tumors and inflammatory diseases. The present study utilized a network pharmacology approach, microarray data analysis and molecular docking to predict the key active ingredients and mechanisms of HDW against RA. Eleven active ingredients in HDW and 180 potential anti-RA targets were identified. The ingredients-targets-RA network showed that stigmasterol, beta-sitosterol, quercetin, kaempferol, and 2-methoxy-3-methyl-9,10-anthraquinone were key components for RA treatment. KEGG pathway results revealed that the 180 potential targets were inflammatory-related pathways with predominant enrichment of the AGE-RAGE, TNF, IL17, and PI3K-Akt signaling pathways. Screened through the PPI network and with Cytoscape software, the expression levels of 8 hub targets, RELA, TNF, IL6, TP53, MAPK1, AKT1, IL10, and ESR1, were determined using 3 Gene Expression Omnibus (GEO) datasets and The Human Protein Atlas (HPA). Molecular docking was used to identify the binding of 5 key components and the 8 related-RA hub targets, providing evidence for a clinical effect of HDW on RA and a research basis for further investigation into the active ingredients and mechanisms of HDW against RA.
The noninvasive echocardiographic assessment of right ventricular myocardial work in a healthy population
Authors: Wu Jian , Huang Xinyi , Huang Kunhui , Gao Qiumei , Tian Yuan , Lin Biqin , Tang Yiruo , Chen Xu , Su Maolong
Published: May 2022

Background: Right ventricular (RV) myocardial work (RVMW) is the latest indicator to assess RV function. Nevertheless, the physiological determinants of RVMW have not been studied and reference ranges for normal RVMW indices remain to be established.Methods: A total of 263 healthy volunteers (median age 34 years, 38% men) were prospectively enrolled. RVMW indices were analysed by RV pressure-strain loop (RVPSL) in a specific software.Results: The lowest values for RV global work index (RVGWI) were 133 mmHg% and 206 mmHg%, RV global constructive work (RVGCW) were 165 mmHg% and 241 mmHg%, and RV global work efficiency (RVGWE) were 78% and 83% in men and women, respectively. The highest values for RV global wasted work (RVGWW) in men and women were 67 mmHg% and 79 mmHg%, respectively. RVGWI and RVGCW were significantly lower in men than in women. RVGWW significantly increased according to age in men and women. RVGWE significantly decreased with age in women. Multivariable analysis revealed RVGWI, RVGCW and RVGWE increased along with increasing pulmonary artery systolic pressure (PASP).Conclusions: Normal reference values of RVMW were obtained by echocardiography. Quantitative data on RVMW could be essential in clinical work and clinical experiments.
Methylome and transcriptome data integration reveals potential roles of DNA methylation and candidate biomarkers of cow Streptococcus uberis subclinical mastitis
Authors: Wang Mengqi , Bissonnette Nathalie , Laterrière Mario , Dudemaine Pier-Luc , Gagné David , Roy Jean-Philippe , Zhao Xin , Sirard Marc-André , Ibeagha-Awemu Eveline Mengwi
Published: May 2022

Background
Mastitis caused by different pathogens including Streptococcus uberis (S. uberis) is responsible for huge economic losses to the dairy industry. In order to investigate the potential genetic and epigenetic regulatory mechanisms of subclinical mastitis due to S. uberis, the DNA methylome (whole genome DNA methylation sequencing) and transcriptome (RNA sequencing) of milk somatic cells from cows with naturally occurring S. uberis subclinical mastitis and healthy control cows (N = 3/group) were studied.
Results
Globally, the DNA methylation levels of CpG sites were low in the promoters and first exons but high in inner exons and introns. The DNA methylation levels at the promoter, first exon and first intron regions were negatively correlated with the expression level of genes at a whole-genome-wide scale. In general, DNA methylation level was lower in S. uberis-positive group (SUG) than in the control group (CTG). A total of 174342 differentially methylated cytosines (DMCs) (FDR < 0.05) were identified between SUG and CTG, including 132237, 7412 and 34693 in the context of CpG, CHG and CHH (H = A or T or C), respectively. Besides, 101612 methylation haplotype blocks (MHBs) were identified, including 451 MHBs that were significantly different (dMHB) between the two groups. A total of 2130 differentially expressed (DE) genes (1378 with up-regulated and 752 with down-regulated expression) were found in SUG. Integration of methylome and transcriptome data with MethGET program revealed 1623 genes with significant changes in their methylation levels and/or gene expression changes (MetGDE genes, MethGET p-value < 0.001). Functional enrichment of genes harboring ≥ 15 DMCs, DE genes and MetGDE genes suggest significant involvement of DNA methylation changes in the regulation of the host immune response to S. uberis infection, especially cytokine activities. Furthermore, discriminant correlation analysis with DIABLO method identified 26 candidate biomarkers, including 6 DE genes, 15 CpG-DMCs and 5 dMHBs that discriminated between SUG and CTG.
Conclusion
Finally, the integration of methylome and transcriptome of milk somatic cells suggests the possible involvement of DNA methylation changes in the regulation of host immune response to subclinical mastitis due to S. uberis. The presented genetic and epigenetic biomarkers could contribute to the design of management strategies of subclinical mastitis and breeding for mastitis resistance.
Association Between Dietary Acid Load and Risk of Gastric Cancer: A Case-control Study
Authors: Dehghani Farimah , Toorang Fatemeh , Sasanfar Bahareh , Narmcheshm Saba , Hadji Maryam , Zendehdel Kazem , Esmaillzadeh Ahmad
Published: May 2022

Gastric cancer (GC) is the fifth most prevalent cancer worldwide. At the same time, it is the third cause of death from cancer. This indicated the low survival rate of this cancer. This emphasizes the importance of prevention and early detection strategies. We found a positive association between dietary acid load and gastric cancer odds using a case-control design. Adherence to a healthy diet with an appropriate acid-base balance might help prevent cancer-related conditions.
Unmet health care needs among pregnant women during COVID-19 pandemic and its determinants in Iran: prevalence, reasons and health policy implications
Authors: Afkhamzadeh Abdorrahim , Shokri Azad , Safari Hossein , Nouri Elham , Bolbanabad Amjad Mohamadi , Amirihosseini Shina , Piroozi Bakhtiar
Published: May 2022

Background: The aim of this study was to assess unmet needs for health care (UNHC) and its causes during COVID-19 pandemic among pregnant women in Iran. Methods: This cross-sectional study was performed among pregnant women in Kurdistan province in 2020 with a sample size of 800 people who were selected by multi-stage sampling method. Data were collected by a questionnaire. Chi-square test was used to examine the relationship between independent variables including demographic variables with dependent variables of perceived need and benefiting from health services. Also, multiple logistic regression was used to determine the relationship between independent variables and outcome variable. Statistical tests were performed using STATA software package.Results: The highest UNHC were related to dental services with 66%, rehabilitation services with 58.6% and psychological services with 43.2% and the lowest were related to hospital services with 12%, midwifery services with 15.6% and physician visit services with 39.1%. The most important reasons for UNHC were fear of getting COVID-19 and the cost of the service. The variables of age group and spouse education for physician visit services; age group for midwifery services; age group, education and employment status for dental services; age group, supplementary insurance and economic status for rehabilitation services; and age group and economic status for psychological services were significantly associated with UNHC (P<0.05).Conclusion: A significant percentage of health care needs of pregnant women were unmet, for which the fear of getting COVID-19 and financial barriers were the main reasons.
Stability of Gauss-Seidel type monotone iterative solutions for nonlinear nonlocal functional convection reaction-diffusion boundary value problems
Authors: Ma Tianfu
Published: May 2022

In this paper, one kind of Gauss-Seidel type monotone iterative solution and its stability for a class of nonlinear convection reaction-diffusion problems with functional reaction terms and nonlocal boundary conditions are studied by the upper and lower solutions method and monotone iterative technique. Using the monotone iterative method, the maximal and minimal solutions, the existence and uniqueness, consistent convergence and stability analysis of the solutions are presented together. Finally, the effectiveness and applicability of the Gauss-Seidel type monotone iterative algorithm are verified by a simple model.
Depression and anxiety among children and adolescents during the COVID-19 pandemic in Europe: A systematic review protocol
Authors: LUDWIG-WALZ Helena , DANNHEIM Indra , PFADENHAUER Lisa M. , FEGERT Jörg M. , BUJARD Martin
Published: May 2022

Some studies point to a high mental health burden among children and adolescents (CA) during the COVID-19 pandemic, particularly concerning depression and anxiety (DA). However, the quality of existing studies and some results are heterogeneous. Research gaps exist regarding (1) a high-quality summarizing overview on studies with (2) a pre-pandemic baseline on the impact of the COVID-19 pandemic on DA among CA in Europe. Therefore, the planned systematic review (SR) aims to close these gaps. SR was registered in the Prospective Register of Systematic Reviews (PROSPERO) and the protocol was prepared in accordance to the Preferred Reporting Items for Systematic review and Meta-Analysis Protocols (PRISMA-P) statement. Search strategy was peer reviewed. Systematic search was conducted in six databases (MEDLINE, EMBASE, PsycINFO, Cochrane Central Register of Controlled Trials, Web of Science and WHO COVID-19 database). Risk of bias (RoB) will be assessed using the RoB instrument for non-randomized studies of exposures. Certainty of evidence will be evaluated by using the GRADE approach adapted to the use of non-randomized studies. Also, subgroup analysis, sensitivity analysis, and publication bias will be performed.Results of this SR could contribute to determine the actual overall prevalence of DA among CA in European countries with an expert opinion on the clinical relevance and extrapolation of DA in CA in the next years. In addition, it aims identifying geographic and pandemic-policy differences and designate particularly vulnerable risk groups within European CA.
Growth hormone inhibits adipogenic differentiation and induces browning in bovine subcutaneous adipocytes
Authors: Li Tingting , Bai Hui , Fang Haoyuan , Yang Liang , Yan Peishi
Published: May 2022

It is well established that growth hormone (GH) has the ability to stimulate lipolysis. The effects of GH on adipocyte differentiation and browning have not been clearly described. Therefore, the present study aimed to elucidate the role of GH in the differentiation and browning of bovine subcutaneous adipocytes as well as its underlying molecular mechanisms. In this study, we demonstrated that GH inhibited lipid accumulation and decreased the expression levels of adipogenic key genes (SCD1, SREBP1, PPARγ, and CEBPα) during adipocyte differentiation. Moreover, we observed that the inhibitory effect of GH on the early stage of adipocyte differentiation (0–2 days) was stronger than that on the later stage of adipocyte differentiation (2–8 days). We also found that GH promoted the expression levels of browning-related genes such as uncoupling protein 1 (UCP1) in mature adipocytes. Concurrently, GH promoted mitochondrial biogenesis and increased the expression levels of mitochondrial biogenesis-related genes. In addition, GH promoted phosphorylation of signal transducers and activator of transcription 5 b (STAT5B) and contributed to translocation of STAT5B to nucleus. After blocking the expression of STAT5B protein, GH weakened the inhibition of adipogenic key genes and reduced the promotion of browning-related genes in bovine subcutaneous adipocytes. To sum up, our findings indicate that GH inhibits adipocyte differentiation and promotes adipocyte browning by regulating STAT5B in bovine subcutaneous adipocytes.