bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetically Engineered Filamentous Bacteriophages Displaying TGF-β1 Promote Angiogenesis in 3D Microenvironments
Authors: Baek In-Hyuk , Helms Volkhard , Kim Youngjun
Published: October 2024

Combined 3D cell culture in vitro assays with microenvironment-mimicking systems are effective for cell-based screening tests of drug and chemical toxicity. Filamentous bacteriophages have diverse applications in material science, drug delivery, tissue engineering, energy, and biosensor development. Specifically, genetically modified bacteriophages have the potential to deliver therapeutic molecules or genes to targeted tumor tissues. The engineered bacteriophages in this study significantly enhanced endothelial cell migration and tube formation within the extracellular matrix (ECM). Compared to TGF-β1 alone and non-modified phages, the presence of TGF-β1 on the bacteriophages demonstrated superior performance as a continuous stimulant in the microenvironment, effectively promoting these angiogenic processes. Assays, including RT-qPCR, ELISA, and fluorescence microscopy, confirmed the expression of angiogenic markers such as CD31, validating the formation of 3D angiogenic structures. Our findings indicate that the TGF-β1 displayed by bacteriophages likely acted as a chemotactic factor, promoting the migration, proliferation, and tube formation of endothelial cells (ECs) within the ECM. Although direct contact between ECs and bacteriophages was not explicitly confirmed, the observed effects strongly suggest that TGF-β1-RGD bacteriophages contributed to the stimulation of angiogenic processes. The formation of angiogenic structures by ECs in the ECM was confirmed as three-dimensional and regulated by the surface treatment of microfluidic channels. These results suggest that biocompatible TGF-β1-displaying bacteriophages could continuously stimulate the microenvironment in vitro for angiogenesis models. Furthermore, we demonstrated that these functionalized bacteriophages have the potential to be utilized as versatile biomaterials in the field of biomedical engineering. Similar strategies could be applied to develop angiogenic matrices for tissue engineering in in vitro assays.
Seasonal Characteristics of Air–Sea Exchanges over the South Coast of Matara, Sri Lanka
Authors: Lu Xuancheng , Luo Yao , Wang Dongxiao , Yao Jinglong , Priyadarshana Tilak , Zhang Zhenqiu , Zhou Fenghua
Published: October 2024

Air–sea exchanges play a crucial role in intense weather events over Sri Lanka, particularly by providing the heat and moisture that fuel heavy rainfall. We present a year-round dataset of meteorological observations from the southern shoreline of Sri Lanka in the equatorial Indian Ocean for 2017, aiming to investigate its seasonal characteristics and evaluate the performance of reanalysis data in this region. The observations reveal distinct diurnal and seasonal patterns. During the winter and spring, higher shortwave (646.2 W/m2) and longwave radiation (−86.9 W/m2) are coupled with higher temperatures (30.6 °C) and lower humidity (67.4% at noon). In contrast, the Indian summer monsoon period features reduced shortwave (579.8 W/m2) and longwave radiation (−58.6 W/m2), lower temperatures (29.2 °C), higher humidity (over 79.7%), and stronger winds (6.25 m/s). The observations were compared with the ERA5 reanalysis dataset to evaluate the regional performance. The reanalysis data correlated well with the observed data for the radiation, temperature, and sensible heat flux, although notable deviations occurred in terms of the wind speed and latent heat flux. During the impact of Tropical Cyclone Ockhi, the reanalysis data tended to underestimate both the wind speed and precipitation. This dataset will provide vital support for studies on monsoons and coastal atmospheric convection, as well as for model initialization and synergistic applications.
Can Land Transfer Alleviate Multidimensional Relative Poverty in Rural Areas?
Authors: Wu Benjian , Rong Yiyuan , Liang Xinyu , Zhu Haibo , Shi Xue
Published: October 2024

After eliminating absolute poverty, China’s anti-poverty efforts have shifted towards multidimensional relative poverty. This study uses microdata from the Chinese Academy of Agricultural Sciences (CAAS) from 2012 to 2021, applies the Kakwani index to construct a multidimensional relative poverty indicator, and uses an endogenous transformation model to analyze the impact of land transfer on multidimensional relative poverty in rural areas. The study found that (1) land transfer can effectively reduce the multidimensional relative poverty of farm households. (2) Land transfer has a better mitigating effect on young farmers, farmers with higher literacy levels, and farmers who have not acute poverty. (3) Land transfer alleviates multidimensional relative poverty by increasing agricultural mechanization, business income, off-farm employment, and wage income. (4) The effects of land transfer out and land transfer in on the dimensions of multidimensional relative poverty are heterogeneous.
Study on Wear and Corrosion Resistance of Ni60/WC Coating by Laser Cladding on Reciprocating Pump Plunger: Comparison with Flame-Sprayed Plungers
Authors: Wang Xiaogang , Qi Jingjing , Zhang Hao , Zhao Ning , Shao Zhangbin , Wang Shuyao
Published: October 2024

Reciprocating pumps are widely used in the current oil extraction process, and the plunger is a vulnerable part of these pumps that directly determines the service life of the reciprocating pump. To improve the service life of plungers, Ni60/WC coatings were applied to the surface of 45-steel plungers via laser cladding technology to improve wear and corrosion resistance. Defect-free and dense Ni60/WC coatings were successfully applied to the plunger surface with strong metallurgical bonding between the coating and the substrate. The coating consists mainly of a γ-(Ni, Fe) phase, which contains isotropic and isotropic-like crystals, dendritic crystals, and columnar crystals in the top, middle, and bottom regions of the coating, respectively. The service performance of the laser cladding coating was compared to the flame-sprayed plunger, which is widely used, and the laser cladding coating has a microhardness of up to 821.8 HV0.5, which is higher than that of the flame-sprayed coating (545.5 HV0.5) and the 45-steel substrate (200 HV0.5). The laser cladding coating has a lower friction coefficient and a smaller volumetric wear rate, and the corrosion current density and corrosion rate in the NaCl solution are 2.52 × 10−7 A/cm2 and 2.96 × 10−3 mmPY, respectively, which indicates superior corrosion resistance to the flame-sprayed coating and the substrate. The laser cladding of reciprocating pump plunger surfaces has a significantly improved comprehensive performance and is a promising way to increase the service life of reciprocating pumps.
Numerical Optimization of Functionally Graded Ti-HAP Material for Tibial Bone Fixation System
Authors: Szymkiewicz Krzysztof
Published: October 2024

Functionally graded materials (FGMs) are heterogeneous composites characterized by outstanding properties. They are built from two or more components with a gradient distribution of chemical composition along a given direction. A promising graded material for biomedical engineering as an implant could be a FGM made of titanium (Ti) and hydroxyapatite (HAP). It would allow us to counteract the difference between the stiffness modulus of pure titanium and bone tissue. Moreover, it can be a good solution to the problem of stress shielding for bone fixation plates made of conventional titanium or steel. The presented paper aims to perform micromechanical modeling and optimization of a functionally Ti-HAP graded plate, followed by numerical analysis of a fractured tibia stabilization system under specific boundary conditions. Finite element analysis was performed using ANSYS Workbench 2021 software. The models of the FGM plate and tibial fixation system were made using the Space Claim tool. The ANSYS software allowed the optimization of the model considered and the selection of the appropriate structural parameters of the FGM Ti-HAP material. In general, the results proved that the osteosynthesis plate built of graded Ti-HAP material resulted in lower bone stress compared to titanium and steel plates. The results obtained confirmed the validity of the design and the possibility to use functionally graded Ti-HAP bone fixation plates.
Research of Optical Properties and Biocompatibility in Different Zones of Multilayered Translucent Zirconia on Hydrothermal Aging
Authors: Kim Ju-Hyun , Yang Ye-Jin , Ahn Jin-Soo , Shin Soo-Yeon , Lee Jung-Hwan , Choi Yu-Sung
Published: October 2024

Objective: We assessed the changes in optical properties and biocompatibility of transition zones in multilayered translucent monolithic zirconia exposed to prolonged hydrothermal aging and compared the results to those with different yttrium oxide contents. Materials and Methods: Four types of zirconia blocks from IPS e.max ZirCAD were used: 3Y-TZP e.max ZirCAD LT (ZL), 4Y-TZP e.max ZirCAD MT (ZM), 5Y-TZP e.max ZirCAD MT Multi (ZT), and 3Y/5Y-TZP e.max ZirCAD Prime (ZP). A total of 120 specimens (15.0 mm diameter and 1.5 mm height) were fabricated and divided into three groups (n = 10). The aging process for the specimens was conducted in an autoclave set to 134 °C and 0.2 MPa, with durations of 0 h (control), 5 h (first aged), and 10 h (second aged). The optical properties and biocompatibility were analyzed, followed by a statistical analysis of the data (α = 0.05). Results: Before and after aging, ZL and ZP exhibited the lowest color changes. ZT exhibited the highest average transmittance and translucency parameter values, while ZL had the lowest. The water contact angle test showed the highest value in ZM and lowest in ZL across all the aging stages. ZL, ZM, and ZP showed a considerable decrease in the water contact angle; however, ZT did not. A cell counting kit-8 assay showed ZL had the highest value, while ZM had the lowest. A filamentous actin test exhibited the highest value in ZL and lowest in ZM. In the vinculin analysis, ZL and ZT exhibited the lowest values, whereas ZM and ZP had the highest. Conclusion: 3Y/5Y-TZP exhibited a balanced performance across critical parameters, such as color stability, translucency, and biocompatibility, aligning with 3Y-TZP. While 5Y-TZP demonstrated superior translucency, it confirmed the lowest color stability, whereas 3Y-TZP achieved the highest biocompatibility. These properties provide clinicians with a reliable material option that ensures superior esthetic outcomes and long-term prognosis, ultimately contributing to improved patient satisfaction and clinical longevity.
A Bivariate Power Lindley Survival Distribution
Authors: Martínez-Flórez Guillermo , Arnold Barry C. , Gómez Héctor W.
Published: October 2024

We introduce and investigate the properties of new families of univariate and bivariate distributions based on the survival function of the Lindley distribution. The univariate distribution, to reflect the nature of its construction, is called a power Lindley survival distribution. The basic distributional properties of this model are described. Maximum likelihood estimates of the parameters of the distribution are studied and the corresponding information matrix is identified. A bivariate power Lindley survival distribution is introduced using the technique of conditional specification. The corresponding joint density and marginal and conditional densities are derived. The product moments of the distribution are obtained, together with bounds on the range of correlations that can be exhibited by the model. Estimation of the parameters of the model is implemented by maximizing the corresponding pseudo-likelihood function. The asymptotic variance–covariance matrix of these estimates is investigated. A simulation study is performed to illustrate the performance of these parameter estimates. Finally some examples of model fitting using real-world data sets are described.
Beyond Earth: Harnessing Marine Resources for Sustainable Space Colonization
Authors: Lemos Marco
Published: October 2024

The quest for sustainable space exploration and colonization is a challenge in its infancy, which faces scarcity of resources and an inhospitable environment. In recent years, advancements in space biotechnology have emerged as potential solutions to the hurdles of prolonged space habitation. Taking cues from the oceans, this review focuses on the sundry types of marine organisms and marine-derived chemicals that have the potential of sustaining life beyond planet Earth. It addresses how marine life, including algae, invertebrates, and microorganisms, may be useful in bioregenerative life support systems, food production, pharmaceuticals, radiation shielding, energy sources, materials, and other applications in space habitats. With the considerable and still unexplored potential of Earth’s oceans that can be employed in developing space colonization, we allow ourselves to dream of the future where people can expand to other planets, not only surviving but prospering. Implementing the blend of marine and space sciences is a giant leap toward fulfilling man’s age-long desire of conquering and colonizing space, making it the final frontier.
Improved Isolation Optimizes Downstream Application of Extracellular Vesicles Derived from Mycobacterium tuberculosis
Authors: Wang Wenjing , Hou Yue , Zhang Jingfang , Sun Zhaogang , Sun Hong
Published: October 2024

Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, secretes extracellular vesicles (EVs), which may play an important role in mediating interactions between bacteria and host cells. Mtb EVs can be isolated by means of various techniques, which differ in terms of their effectiveness. In the present study, we found that an exosome isolation kit (EI) yielded higher numbers of EVs than either differential centrifugation (DC) or exosome detection via an ultrafast-isolation system (EXODUS). We also found that the EXODUS method revealed a greater abundance of H37Rv components within EVs, compared with the DC and EI methods. Analysis of the downstream application of H37Rv EVs revealed their internalization by RAW264.7 macrophages, peaking at 6 h, with subsequent activation of the TLR2 signaling pathway leading to the expression of inflammatory cytokines including IL-6 and TNF-α. It was also found that H37Rv EVs could cross the blood–brain barrier (BBB) and enter the brain, peaking at 12 h post-injection, eliciting an inflammatory response in the cerebral parenchyma, cerebellum, and hippocampus that persisted for up to 6 days. These findings offer novel insights into the pathogenesis of Mtb-induced diseases and may guide the development of therapeutic strategies.
Molecular Biology for Diagnosis of Congenital and Neonatal Infections in the Cerebrospinal Fluid of Newborns from a Brazilian Tertiary Hospital
Authors: Zimmerman Suzana Ferreira , de Lima Rodrigo Gonçalves , Morcillo André Moreno , Bonon Sandra Helena Alves , Marba Sergio Tadeu Martins
Published: October 2024

The risk of infection transmission from mother to fetus depends on the pathogen. TORCH agents cause some neuroinfections, including Toxoplasmosis, rubella, Cytomegalovirus, herpes simplex 1 and 2, and others (Varicella Zoster, Parvovirus B-19, Epstein–Barr virus, and Zika virus). The consequences can be stillbirth, prematurity, uterine growth restriction, and congenital malformations. The detection of DNA/RNA from CSF by molecular methods is a marker of the involvement of congenital infection in the central nervous system. This study aimed to identify the frequency of these pathogens in CSF samples from newborns (1 to 28 days old) at a tertiary hospital, using PCR, and determine the clinical consequences. Methods: This was a prospective descriptive study involving the molecular analysis of 151 CSF samples from neonates, collected for cytological and biochemical diagnosis from 2017 to 2021. After the results and consent from the participants’ caregivers were obtained, the leftover material was sent to the University’s Virology Laboratory and submitted for DNA/RNA extraction and Nested-PCR/RT-PCR. A review of the patients’ medical records and descriptive statistics was performed. This work was approved by the Ethics Committee (CAAE: 86760218.3.0000.5404). Results: A total of 151 CSF samples were obtained, 16 of which were positive (10.6% [95% CI%: 6.18–16.63%]). Two of these were PCR-positive for HSV-1 (1.3%), four for VZV (2.6%), one for CMV (0.67%), two for Toxoplasmosis (1.3%), four for Parvovirus B-19 (2.6%), and four for Zika (2.6%). The proportion of positive PCR results was higher in the group that presented with malformations (25.0% vs. 8.4%, p = 0.040). Conclusions: The pathogens identified by PCR were mostly Zika virus, VZV, and B-19, and these were mainly found in newborns with malformations.
Mediterranean Diet Is a Predictor of Progression of Subclinical Atherosclerosis in a Mediterranean Population: The ILERVAS Prospective Cohort Study
Authors: Rojo-López Marina Idalia , Bermúdez-López Marcelino , Castro Eva , Farràs Cristina , Torres Gerard , Pamplona Reinald , Lecube Albert , Valdivieso José Manuel , Fernández Elvira , Julve Josep , Castelblanco Esmeralda , Alonso Nuria , Antentas Maria , Barranco-Altirriba Maria , Perera-Lluna Alexandre , Franch-Nadal Josep , Granado-Casas Minerva , Mauricio Didac ,   on behalf of the ILERVAS project collaborators
Published: October 2024

Atherosclerotic cardiovascular disease remains a major health issue, often developing silently as subclinical atherosclerotic disease (SAD). The Mediterranean diet (MDiet) is known for its cardiovascular benefits, but the combined influence of both MDiet adherence and physical activity (PA) on SAD progression has not been previously documented. Objective: We aimed to investigate how adherence to a healthy lifestyle, defined as MDiet adherence and PA level, influences SAD progression in subjects from the ILERVAS cohort follow-up. Methods: A study on 3097 participants from the ILERVAS prospective cohort was conducted. MDiet adherence was assessed using the MEDAS score, and PA categories were established using the IPAQ, both categorized into low, moderate, and high levels. Two different lifestyle scores integrating the MDiet and PA categories were built. The presence of atherosclerotic plaques was assessed by carotid and femoral ultrasound examination. Demographic, clinical, and biochemical data were also obtained. Multivariable linear, logistic, and Poisson regression models adjusted for potential confounders were used to analyze the association between the lifestyle scores and SAD progression, as well as the MDiet and PA as separate variables and number of territories with plaque. Results: A healthier lifestyle score did not show an effect on SAD progression. However, a higher MEDAS score was associated with a 3% decrease in the number of territories with plaque (IRR 0.97, 95% CI 0.96–0.99, p < 0.001), suggesting a protective effect of the adherence to the MDiet. PA did not show a significant association (IRR 1.00, 95% CI 1.00–1.00, p = 0.269). Older age, hypertension, dyslipidemia, smoking, and lower eGFR were associated with SAD progression, while the female sex was protective (IRR 0.67, 95% CI 0.63–0.72, p < 0.001). Conclusions: The findings of this study show that higher adherence to the MDiet is associated with reduced incidence of SAD, indicating its potential role in cardiovascular prevention strategies. Although a higher lifestyle score or physical activity levels did not show any significant effect, promoting the MDiet, alongside managing traditional cardiovascular risk factors, could be an effective public health intervention to prevent atherosclerosis and reduce the burden of cardiovascular disease.
Antarctic Krill Euphausia superba Oil Supplementation Attenuates Hypercholesterolemia, Fatty Liver, and Oxidative Stress in Diet-Induced Obese Mice
Authors: Choi Jun-Hui , Park Se-Eun , Kim Seung
Published: October 2024

Background: Several Previous studies indicate that consuming krill oil may aid in reducing hypercholesterolemia and improving cholesterol metabolism. Therefore, our study was designed to investigate the effectiveness of Antarctic krill oil (Euphausia superba) (ESKO) in combating obesity and lowering fat/lipid/cholesterol levels. Methods: The study aimed to investigate the molecular docking model targeting 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) using ESKO-derived eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and astaxanthin. In this study, histological alterations in the liver of the obesity model (ICR male mouse), obesity-related or antioxidant markers in both liver and serum, the molecular mechanisms in HepG2 cells and liver tissue, and HMGCR activity were analyzed. Results: Our findings revealed that a high-fat diet (HFD) significantly led to increased oxidative stress, obesity-related indicators, and cardiovascular-associated risk indices. However, ESKO effectively mitigated HFD-induced oxidative stress, fat accumulation, and the suppression of low-density lipoprotein receptor (LDLR) or activation of related molecular pathways. This was achieved through improvements in metabolic parameters, including CD36/liver X receptor α (LXRα)/sterol regulatory element-binding protein 1c (SREBP1c), proprotein convertase subtilsin/kexin type 9 (PCSK-9), and HMGCR, ultimately ameliorating HFD-induced hypercholesterolemia and obesity. Conclusions: These beneficial findings indicate that ESKO might have significant potential for preventing and treating obesity-related disorders.
Effect of Anthelmintic Treatment on the Agreement Between Real-Time Polymerase Chain Reaction (RT-PCR) and Kato–Katz Microscopic Technique in the Diagnosis of Soil-Transmitted Helminth Infections
Authors: Nguema-Moure Paul Alvyn , Dejon-Agobé Jean Claude , Laclong Lontchi Roméo-Aimé , Manouana Gédéon Prince , Nzamba Maloum Moustapha , Meulah Brice , Manfoumbi Mabicka Danny Carrel , Nzame Ngome Marguerite Emmanuelle , Kremsner Peter Gottfried , Adegnika Ayôla Akim
Published: October 2024

Soil-transmitted helminths (STHs), including Ascaris lumbricoides, hookworm species, and Trichuris trichiura, cause significant morbidity worldwide. For an effective proper control of their morbidity, accurate diagnosis method is needed. To this end, a polymerase chain reaction (PCR) method has been developed, but its use remains limited due to the high cost of its implementation, the resources required, and the lack of qualified technicians. The objective of the present analysis is to assess the agreement between the Kato–Katz microscopy method and quantitative real-time PCR (RT-PCR) in the diagnostic of STHs before and after treatment, to decipher the usefulness of either technique for evaluation of the treatment. Methods: Stool samples were collected before and after three- or six-weeks post-treatment from study participants and analyzed using Kato–Katz and RT-PCR methods for the diagnosis of STHs infections. The cure rate (CR) was estimated according to each diagnostic method. Agreement between CRs was tested using the Kappa statistical test. Results: Agreement between Kato–Katz and RT-PCR methods varied regarding the STH species targeted and was different after treatment compared with before treatment. At baseline, the two diagnostic methods showed a moderate agreement (0.45 < K < 0.5) for all STH species, whereas after treatment, concordance decreased slightly (0.11 ≤ K ≤ 0.14) for A. lumbricoides, remained moderate (0.5 ≤ K ≤ 0.53) for T. trichiura, and went from moderate to absent for hookworms. Conclusion: Our findings showed basically a moderate agreement between the Kato–Katz method and RT-PCR. There is a likely association with a moderate proportion of microscopy-positive cases. Reciprocally, a decrease in agreement after treatment was observed with low microscopy-positive cases after treatment, whereby RT-PCR was more likely to detect positive cases than microscopy. Therefore, the agreement is positively associated with an increasing in the number of samples testing positive.
Effects of Opuntia stricta var. dillenii Extracts Obtained from Prickly Pear and an Industrial By-Product on Maturing Pre-Adipocytes
Authors: Gómez-López Iván , Eseberri Itziar , Krisa Stéphanie , Cano M. Pilar , Portillo María P.
Published: October 2024

Opuntia stricta var. dillenii, a member of the Cactaceae family, produces a fruit known as prickly pear. This fruit is rich in bioactive compounds, including betalains and phenolic compounds, which play an important role in health promotion due to their antioxidant and anti-inflammatory properties. This study aims to investigate the impact of prickly pear extracts obtained from the whole fruit, peel, pulp, and an industrial by-product (bagasse) on the differentiation of 3T3-L1 pre-adipocytes. During the differentiation process, 3T3-L1 pre-adipocytes were treated with prickly pear extracts at concentrations ranging from 10 to 100 μg/mL from day 0 to day 8 post-induction. Moreover, the potential mechanisms justifying the observed effects were assessed by RT-PCR. All extracts led to an increase in both triacylglycerol accumulation and cell number. In conclusion, the analysed extracts demonstrated adipogenic effects in 3T3-L1 maturing pre-adipocytes by increasing the expression of the c/ebp-β, srebf-1, and c/ebp-α genes. Additionally, a potential anti-inflammatory effect was observed through the upregulation of adiponectin.
Directivity Improved Antenna with a Planar Dielectric Lens for Reducing the Physical Size of the On-Vehicle Communication System
Authors: Seo Seongbu , Kim Woogon , Park Hongsik , Seo Yejune , Park Dohyun , Kim Hyoungjong , Lee Kwonhee , Lee Hosub , Kahng Sungtek
Published: October 2024

As the physical size of a communication system for satellites or unmanned aerial vehicles demands to be reduced, a compact antenna with high directivity is proposed as a core element essential to the wireless device. Instead of using a horn or an array antenna, a unit planar antenna is combined with a surface-modulated lens to convert a low antenna gain to a high antenna gain. The lens is not a metal-patterned PCB but is dielectric, which is neither curved nor very wide. This palm-sized lens comprises pixels with different heights from the backside of PolyPhenylene Sulfide (PPS) as the dielectric base. The antenna gain from the unit antenna of 4.5 cm × 4.5 cm is enhanced by 10 dB with the help of a compact dielectric lens of 7.5 cm × 7.5 cm at 24.5 GHz as the frequency of interest. The antenna design is verified by far-field measurement as well as near-field observation, including sensing a metal object behind a blocking wall by using an RF test bench. Moreover, antenna performance is understood by making a comparison with conventional designs of antennas in terms of directivity and physical sizes.
An Analysis of Policy Frameworks on Business Formulization and Disaster Management to Mitigate Flood Loss and Damage Among Informal Businesses in Sri Lanka
Authors: Hewawasam Vindya , Matsui Kenichi
Published: October 2024

The 27th Conference of the Parties to the United Nations Framework Convention on Climate Change in Egypt made a historical decision on disaster loss and damage for vulnerable countries. Even though parties agreed to salvage vulnerable nations with generous support, a fundamental question remains as to the extent to which these vulnerable countries can accurately identify the most vulnerable communities/areas. In Sri Lanka, informal businesses are particularly vulnerable to floods, but their informal status has limited the extent to which they could receive flood protection, including disaster-resilient infrastructure developments. The main objective of this paper, therefore, is to identify challenges that informal businesses have experienced in dealing with flood disaster risks. In doing so, we analyzed government policies and laws that are related to business formalization and disaster management. We also conducted interviews with key informants to verify our data. Our analysis found that the Sri Lankan government requires informal business owners to follow complicated rules to register their businesses. For these owners, who are not highly educated, these processes and fear of high tax rates discouraged registering their businesses. The central government tends to prioritize flood mitigation actions for formalized business areas. Informal businesses are not usually covered by flood insurance and compensation. In conclusion, we emphasize the need to establish widely available legal and administrative support for informal businesses to register. Adopting business continuity plans (BCPs) and keeping standardized business records also help businesses minimize flood loss and damage.
A Novel Spectral Correction Method for Predicting the Annual Solar Photovoltaic Performance Ratio Using Short-Term Measurements
Authors: Daniel-Durandt Francisca Muriel , Rix Arnold Johan
Published: October 2024

A novel spectral-corrected Performance Ratio calculation method that aligns the short-term Performance Ratio calculation to the annual calculated Performance Ratio is presented in this work. The spectral-corrected Performance Ratio allows short-term measurements to reasonably estimate the annual Performance Ratio, which decreases the need for long-term measures and data storage and assists with routine maintenance checkups. The piece-wise empirical model incorporates two spectral variables, a geographical location-based variable, the air mass, a PV-technology-based variable, and a newly defined spectral correction factor that results in a universal application. The spectral corrections show significant improvements, resulting in errors across different air mass and clearness index ranges, as well as temporal resolutions. The results indicate that a spectral correction methodology is possible and a viable solution to estimate the annual Performance Ratio. The results further indicate that by correcting the spectrum, short-term measurements can be used to predict the annual Performance Ratio with superior performance compared to the well-known normal and weather-corrected PR calculation methods. This approach is the first documented effort to address the spectrum’s influence on the utility-scale Performance Ratio calculation from hourly measurements. The empirical formula suggested for the Performance Ratio calculation can be of extreme value for the real-time monitoring of PV systems and enhancing PV power forecasting accuracy when the spectrum is considered instead of its usual omission. The model can be universally applicable, as it incorporates location and technology, marking a groundbreaking start to comprehending and incorporating the spectral influence in utility-scale PV systems. The novel calculation has widespread application in the PV industry, performance modelling, monitoring, and forecasting.
Impact of the Digital Economy on the Green Economy: Evidence from China
Authors: Wang Chenggang , Liu Tiansen , Du Danli , Zhu Yue , Zheng Zuolong , Li Hanchen
Published: October 2024

In recent years, the digital economy and the green economy have become integral parts of China’s economy. Chinese government has been actively expanding its support for the development of these sectors. However, there are still several obstacles hindering the progress of the digital economy and the green economy in China. The objective of this study is to delve into the impact mechanism of the digital economy on the green economy. Moreover, this paper innovatively integrates industrial structure and technological innovation into this impact process. We elucidate the significant roles played by these two mediating variables. Simultaneously, we employ the bidirectional fixed-effect model, baseline regression, and robustness tests to analyze the data from 31 provinces in China between 2012 and 2021. The conclusions of this study are as follows: (1) The development of the digital economy in China contributes to the improvement of the level of the green economy. (2) When the digital economy influences the green economy, the upgrading of industrial structure and technological innovation play significant roles. (3) The impact of the digital economy on the green economy varies across diverse regions in China. This study reveals the impact mechanism of the digital economy on the development of the green economy using variables of industrial structure and technological innovation. It enriches the research content in this field and extends the application scenarios of related research methods. The conclusions provide valuable references for promoting the development of both the digital economy and the green economy in China.
Evaluating the Efficacy of Limestone Powder as a Partial Replacement of Ordinary Portland Cement for the Sustainable Stabilization of Sulfate-Bearing Saline Soil
Authors: Bazarbekova Ayazhan , Shon Chang-Seon , Kissambinova Aizhan , Zhang Dichuan , Kim Jong
Published: October 2024

In today’s era of rapid infrastructure development, ensuring the durability and environmental sustainability of soil subgrades in road construction remains a critical concern. With recent advancements in non-traditional soil stabilizing binders, including environmentally friendly industrial waste materials such as fly ash and slag, there is growing recognition of the potential for limestone powder (LSP), a low-carbon alternative soil stabilizing material, to replace traditional calcium-based additives like ordinary Portland cement (OPC) and lime. However, the full extent of LSP’s efficacy in soil treatment has yet to be fully explored. Therefore, this paper investigates the partial substitution of cement with LSP for stabilizing sulfate-bearing saline sandy soil and assesses its impact on the treated soil samples’ mechanical properties and durability parameters. For this purpose, five stabilized mixes, including a control mix (no stabilizer), were designed, wherein LSP partially replaced 8% of the OPC at 25%, 50%, and 75% substitution levels. A series of laboratory tests were conducted to track the changes in the geochemical properties and the mineralogical compositions and evaluate the stabilized soil samples’ improved mechanical performance and durability parameters. The experimental results show that adding LSP to the cement-treated sulfate-bearing saline soil improved the soil’s mechanical properties and enhanced the soil’s durability parameters. Specifically, it decreased the soil plasticity, improved the soil strength parameters, enhanced the soil stability, and reduced the volumetric swelling and soil moisture susceptibility. In addition to its technical advantages, using LSP, an industrial byproduct, in soil stabilization offers environmental and economic benefits, highlighting its potential as a sustainable solution in engineering practices.
Hydrogeochemical Insights into the Sustainable Prospects of Groundwater Resources in an Alpine Irrigation Area on Tibetan Plateau
Authors: Yang Shaokang , Zhao Zhen , Wang Shengbin , Xiao Shanhu , Xiao Yong , Wang Jie , Wang Jianhui , Yuan Youjin , Ba Ruishou , Wang Ning , Zhang Yuqing , Wang Liwei , Yang Hongjie
Published: October 2024

The Tibetan Plateau is the “Asia Water Tower” and is pivotal for Asia and the whole world. Groundwater is essential for sustainable development in its alpine regions, yet its chemical quality increasingly limits its usability. The present research examines the hydrochemical characteristics and origins of phreatic groundwater in alpine irrigation areas. The study probes the chemical signatures, quality, and regulatory mechanisms of phreatic groundwater in a representative alpine irrigation area of the Tibetan Plateau. The findings indicate that the phreatic groundwater maintains a slightly alkaline and fresh status, with pH values ranging from 7.07 to 8.06 and Total Dissolved Solids (TDS) between 300.25 and 638.38 mg/L. The hydrochemical composition of phreatic groundwater is mainly HCO3-Ca type, with a minority of HCO3-Na·Ca types, closely mirroring the profile of river water. Nitrogen contaminants, including NO3−, NO2−, and NH4+, exhibit considerable concentration fluctuations within the phreatic aquifer. Approximately 9.09% of the sampled groundwaters exceed the NO2− threshold of 0.02 mg/L, and 28.57% surpass the NH4+ limit of 0.2 mg/L for potable water standards. All sampled groundwaters are below the permissible limit of NO3− (50 mg/L). Phreatic groundwater exhibits relatively good potability, as assessed by the entropy-weighted water quality index (EWQI), with 95.24% of groundwaters having an EWQI value below 100. However, the potential health risks associated with elevated NO3− levels, rather than NO2− and NH4+, merit attention when such water is consumed by minors at certain sporadic sampling locations. Phreatic groundwater does not present sodium hazards or soil permeability damage, yet salinity hazards require attention. The hydrochemical makeup of phreatic groundwater is primarily dictated by rock–water interactions, such as silicate weathering and cation exchange reactions, with occasional influences from the dissolution of evaporites and carbonates, as well as reverse cation-exchange processes. While agricultural activities have not caused a notable rise in salinity, they are the main contributors to nitrogen pollution in the study area’s phreatic groundwater. Agricultural-derived nitrogen pollutants require vigilant monitoring to avert extensive deterioration of groundwater quality and to ensure the sustainable management of groundwater resources in alpine areas.
The Relationship Between the Sustainability of Brand Marketing Communications and the Well-Being of the Saudi Consumer
Authors: AL Hilal Norah Saud H.
Published: October 2024

This study examined the relationship between brand sustainability and Saudi consumer well-being based on the PEOM model of marketing communications and PERMA well-being dimensions, and the relationship between brand sustainability and Saudi consumer well-being. This study used a quantitative approach and employed a descriptive analytical approach, with a field survey focusing on the PEOM and PERMA dimensions, as well as brand sustainability. This study collected primary data from Saudi customers in the first quarter of the year 2024. The results indicated that the economic dimension of brand sustainability was more important to consumers, followed by social and environmental dimensions. Among the preferred marketing communication channels, earned media achieved the highest score, followed by owned and paid media. Engagement and positive emotions emerged as the most prominent well-being dimensions. This study identified significant relationships between Saudi consumer well-being and marketing communication tools, Saudi consumer well-being and brand sustainability dimensions, and marketing communication tools and brand sustainability, emphasizing the importance of demographic factors. These findings suggest that reallocating marketing budgets to focus on earned and owned media can enhance consumer engagement and well-being. This study underscores the essential role of integrated marketing communication strategies within the framework of the PERMA model in advancing consumer well-being and brand sustainability.
Optimizing Evacuation Efficiency in Buildings: A BIM-Based Automated Approach to Sustainable Design
Authors: Godes Cherry Rose , Rodrigazo Shanelle Aira , Cho Junhwi , Song Yooseob , Yeon Jaeheum
Published: October 2024

This study addresses the challenge of optimizing fire evacuation efficiency in complex buildings by investigating the impact of automating corridor dimension adjustments on reducing evacuation congestion. A Building Information Modeling (BIM)-based approach using Autodesk Revit 2024, Dynamo version 2.17, and Thunderhead Pathfinder 2023 simulations was employed to test this hypothesis. The results show that automated adjustments in hallways have a significant positive impact on evacuation efficiency in the majority of building floor corridor segments. These findings highlight the potential for dynamic design modifications to enhance building safety and sustainability. Future research will focus on refining this approach for diverse building layouts and occupant behaviors.
On Knots with Cyclic Symmetries
Authors: Chbili Nafaa
Published: October 2024

This review paper investigates the relationship between symmetries of knots and links in the three-dimensional sphere, with a focus on cyclic symmetries, and their associated polynomial invariants. It examines the behavior of these polynomials in the case where the knot is set-wise fixed by the action of finite cyclic group on the 3-sphere. The study highlights how these invariants can obstruct the existence of such symmetries, providing valuable insights into the topological properties of these knots. The paper reviews established results on polynomial criteria for periodicity and their extensions to freely periodic links. It also explores generalizations to study the symmetries of virtual knots and spatial graphs.
Genomic Sequence of the Threespine Stickleback Iridovirus (TSIV) from Wild Gasterosteus aculeatus in Stormy Lake, Alaska
Authors: Yoxsimer Alyssa M. , Offenberg Emma G. , Katzer Austin Wolfgang , Bell Michael A. , Massengill Robert L. , Kingsley David M.
Published: October 2024

The threespine stickleback iridovirus (TSIV), a double-stranded DNA virus, was the first megalocytivirus detected in wild North American fishes. We report a second occurrence of TSIV in threespine stickleback (Gasterosteus aculeatus) from Stormy Lake, Alaska, and assemble a nearly complete genome of TSIV. The 115-kilobase TSIV genome contains 94 open reading frames (ORFs), with 91 that share homology with other known iridoviruses. We identify three ORFs that likely originate from recent lateral gene transfers from a eukaryotic host and one ORF with homology to B22 poxvirus proteins that likely originated from a lateral gene transfer between viruses. Phylogenetic analysis of 24 iridovirus core genes and pairwise sequence identity analysis support TSIV as a divergent sister taxon to other megalocytiviruses and a candidate for a novel species designation. Screening of stickleback collected from Stormy Lake before and after a 2012 rotenone treatment to eliminate invasive fish shows 100% positivity for TSIV in the two years before treatment (95% confidence interval: 89–100% prevalence) and 0% positivity for TSIV in 2024 after treatment (95% confidence interval: 0 to 3.7% prevalence), suggesting that the rotenone treatment and subsequent crash and reestablishment of the stickleback population is associated with loss of TSIV.
A Novel and Rapid Selective Viral Genome Amplification and Sequencing Method for African Swine Fever Virus
Authors: Licheri Matthias , Licheri Manon Flore , Mehinagic Kemal , Ruggli Nicolas , Dijkman Ronald
Published: October 2024

African swine fever virus (ASFV) is the etiological agent of African swine fever, a highly contagious hemorrhagic disease affecting both wild boars and domestic pigs with lethality rates up to 100%. Until now, the most effective measure to prevent an outbreak of ASFV was early detection. In this situation, whole genome sequencing (WGS) allows the gathering of detailed information about the identity and epidemiology of the virus. However, due to the large genome size and complex genome ends, WGS is challenging. Current WGS workflows require either elaborate enrichment methods or are based on tiled PCR approaches, which are susceptible to genetic differences between ASFV strains. To overcome this, we developed a novel approach for WGS of ASFV, using the Phi29 DNA polymerase-based multiple displacement amplification in combination with only seven primers. Furthermore, we applied an alkaline-based DNA denaturation step to significantly increase the number of viral reads, which resolves the near-full genome of ASFV. This novel isothermal WGS approach can be used in authorized laboratories for the genomic epidemiological analysis of ASFV outbreaks caused by different genotypes.
Buckling and Ultimate Bearing Capacity of Steel Pipes Jacked in Hard Rocks: A Case Study of a Water Pipeline Project in Zhongshan
Authors: Zhao Rusen , Chen Zhidong , Feng Dinghua , Liu Qiping , Wen Peiwen , Yang Hongwei
Published: October 2024

Steel jacking pipes are potentially prone to buckling instability, a phenomenon that has received limited attention in hard rock formations. This study reports on the field monitoring of a water pipeline project in Zhongshan City, where the circumferential and hoop strains of steel pipe segments jacked in hard rocks were recorded. The buckling deformation observed during steel pipe jacking, as well as the critical buckling load, was analyzed with the aid of numerical simulations using finite element software. The initial defect for the post-buckling analysis of the steel pipe was selected as the first-order buckling mode. Field monitoring revealed that the loading conditions experienced by the steel pipe segments during the jacking process are complex, leading to significant deformation. Throughout the monitoring process, axial stress at each measurement point underwent tensile-compressive transitions. Numerical results showed the actual critical buckling load increases with wall thickness at a constant length-to-diameter ratio, which is significant for short pipes. For pipes with the same wall thickness and outer diameter, the actual critical buckling load of long pipes is significantly lower than that of short pipes. Additionally, initial defects were found to significantly reduce the actual critical buckling load of the steel pipe. Furthermore, the actual critical buckling load of long pipes is much lower than their yield load, whereas, for short pipes, the critical buckling load is limited by their yield load. Measures for managing buckling deformation of steel pipes in situ were also reported. The findings on critical buckling load and the countermeasures for managing buckling in situ would be valuable for the design and construction stages of similar projects employing pipe-jacking technology in hard rock formations.
Current issues of using metronidazole in clinical practice. Effects and directions of clinical application
Authors:   FSBEI CPE Russian Medical Academy of Continuous Medical Education of the Ministry of Health of Russia, FSAEI HE Pirogov Russian National Research Medical University, Moscow, Maksimov M. L. , Shikaleva A. A. ,   Kazan State Medical Academy – Branch of the FSBEI CPE Russian Medical Academy of Continuous Professional Education of the Ministry of Health of Russia, Kazan, Bontsevich R. A. ,   Kazan State Medical Academy – Branch of the FSBEI CPE Russian Medical Academy of Continuous Professional Education of the Ministry of Health of Russia, Kazan; FSBEI HE Mari State University, Yoshkar-Ola
Published: October 2024

Metronidazole is an important drug in clinical and outpatient practice due to its
broad antibacterial and antiprotozoal properties. Metronidazole is effective against anaerobic
bacteria and some protozoa such as Trichomonas vaginalis and Giardia lamblia. This
makes it indispensable in the treatment of infections of various etiologies. The review article
contains current information about the clinical pharmacology of metronidazole and its place
in the pharmacotherapy of infectious diseases. General and specific issues of the use of
metronidazole in the practice of GPs, surgeons, obstetrician-gynecologists, and other specialists
are considered. Information is provided on the possibilities and indications for the
use of metronidazole in the eradication of Helicobacter pylori, in radiation therapy for cancer
patients, dermatovenereology, dentistry, treatment of protozoal infections, as well as in alcoholism.
Metronidazole is often used in combination with other antibiotics to enhance the
antimicrobial effect, especially in cases of mixed infections or when it is necessary to cover
a wider range of pathogens. In surgical practice, metronidazole is used to prevent infectious
complications after operations on the abdominal and pelvic organs, where the risk of anaerobic
infection is high. In an environment of increasing antibiotic resistance, metronidazole
remains an effective treatment against many anaerobic pathogens, highlighting its place in
modern practice.