bluuebunny/crossref_metadata_2025 huggingface dataset:
Physiotherapeutic Protocol and ZnO Nanoparticles: A Combined Novel Treatment Program against Bacterial Pyomyositis
Authors: El-Shaer Hesham , Elwakil Bassma H. , Bakr Basant A. , Eldrieny Ahmed M. , El-Khatib Mostafa , Chong Khim Phin , Abo Gazia Amr A.
Published: September 2022

Myositis tropicans or pyomyositis is a muscle inflammation resulting from a bacterial infection of skeletal muscle (commonly caused by Staphylococcus aureus) that usually leads to hematogenous muscle seeding. The present study was designed to estimate the role of ZnO-NPs and a physiotherapeutic program in the management of induced biceps femoris atrophy in rats through histological, biochemical, and radiological examinations at different time intervals. At the beginning, several bacterial strains were evaluated through a proteolytic enzyme activity assay and the highest activity was recorded with the Staphylococcus aureus strain. ZnO-NPs were synthesized with the arc discharge method with an average size of 19.4 nm. The antibacterial activity of ZnO-NPs was investigated and it was revealed that the prepared ZnO-NPs showed a minimum inhibitory concentration of 8 µg/mL against the tested bacterium. The cytotoxicity of the prepared ZnO-NPs was tested in C2C12 myoblast cells, and it was elaborated that CC50 was 344.16 µg/mL. Biceps femoris pyomyositis was induced with a potent strain (Staphylococcus aureus); then, a physiotherapeutic program combined with the prepared ZnO-NPs treatment protocol was applied and evaluated. The combined program claimed antibacterial properties, preventing muscle atrophy, and resulted in the most comparable value of muscle mass.
Functional Gait Assessment Using Manual, Semi-Automated and Deep Learning Approaches Following Standardized Models of Peripheral Nerve Injury in Mice
Authors: Umansky Daniel , Hagen Kathleen M. , Chu Tak Ho , Pathiyil Rajesh K. , Alzahrani Saud , Ousman Shalina S. , Midha Rajiv
Published: September 2022

Objective: To develop a standardized model of stretch–crush sciatic nerve injury in mice, and to compare outcomes of crush and novel stretch–crush injuries using standard manual gait and sensory assays, and compare them to both semi-automated as well as deep-learning gait analysis methods. Methods: Initial studies in C57/Bl6 mice were used to develop crush and stretch–crush injury models followed by histologic analysis. In total, 12 eight-week-old 129S6/SvEvTac mice were used in a six-week behavioural study. Behavioral assessments using the von Frey monofilament test and gait analysis recorded on a DigiGait platform and analyzed through both Visual Gait Lab (VGL) deep learning and standardized sciatic functional index (SFI) measurements were evaluated weekly. At the termination of the study, neurophysiological nerve conduction velocities were recorded, calf muscle weight ratios measured and histological analyses performed. Results: Histological evidence confirmed more severe histomorphological injury in the stretch–crush injured group compared to the crush-only injured group at one week post-injury. Von Frey monofilament paw withdrawal was significant for both groups at week one compared to baseline (p < 0.05), but not between groups with return to baseline at week five. SFI showed hindered gait at week one and two for both groups, compared to baseline (p < 0.0001), with return to baseline at week five. Hind stance width (HSW) showed similar trends as von Frey monofilament test as well as SFI measurements, yet hind paw angle (HPA) peaked at week two. Nerve conduction velocity (NCV), measured six weeks post-injury, at the termination of the study, did not show any significant difference between the two groups; yet, calf muscle weight measurements were significantly different between the two, with the stretch–crush group demonstrating a lower (poorer) weight ratio relative to uninjured contralateral legs (p < 0.05). Conclusion: Stretch–crush injury achieved a more reproducible and constant injury compared to crush-only injuries, with at least a Sunderland grade 3 injury (perineurial interruption) in histological samples one week post-injury in the former. However, serial behavioral outcomes were comparable between the two crush groups, with similar kinetics of recovery by von Frey testing, SFI and certain VGL parameters, the latter reported for the first time in rodent peripheral nerve injury. Semi-automated and deep learning-based approaches for gait analysis are promising, but require further validation for evaluation in murine hind-limb nerve injuries.
Identification of the Thyrotropin-Releasing Hormone (TRH) as a Novel Biomarker in the Prognosis for Acute Myeloid Leukemia
Authors: Gao Yan , Zhou Jia-Fan , Mao Jia-Ying , Jiang Lu , Li Xue-Ping
Published: September 2022

Acute myeloid leukemia (AML) is a biologically and genetically heterogeneous hematological malignance with an unsatisfactory risk stratification system. Recently, through the novel single-cell RNA sequencing technology, we revealed heterogeneous leukemia myeloblasts in RUNX1-RUNX1T1 AML. Thyrotropin-releasing hormone (TRH), as biomarkers of CD34+CD117bri myeloblasts, were found to be prognostic in RUNX1-RUNX1T1 AML. However, the clinical and genetic features of TRH in AML patients are poorly understood. Here, with data from TCGA AML, TRH was found to be downregulated in patients older than 60 years old, with DNMT3A and NPM1 mutations, while overexpressed in patients with KIT mutations. This was further validated in three other cohorts of primary AML including Beat AML (n = 223), GSE6891 (n = 461), and GSE17855 (n = 237). Furthermore, we demonstrated that the expression of TRH in AML could be used to improve the ELN 2017 risk stratification system. In conclusion, our preliminary analysis revealed that TRH, a novel biomarker for AML patients, could be used to evaluate the survival of AML.
Cisplatin Resistance: Genetic and Epigenetic Factors Involved
Authors: Lugones Yuliannis , Loren Pía , Salazar Luis A.
Published: September 2022

Cisplatin (CDDP) is the drug of choice against different types of cancer. However, tumor cells can acquire resistance to the damage caused by cisplatin, generating genetic and epigenetic changes that lead to the generation of resistance and the activation of intrinsic resistance mechanisms in cancer cells. Among them, we can find mutations, alternative splicing, epigenetic-driven expression changes, and even post-translational modifications of proteins. However, the molecular mechanisms by which CDDP resistance develops are not clear but are believed to be multi-factorial. This article highlights a description of cisplatin, which includes action mechanism, resistance, and epigenetic factors involved in cisplatin resistance.
Deletion of Growth Hormone Secretagogue Receptor in Kisspeptin Neurons in Female Mice Blocks Diet-Induced Obesity
Authors: Conde Kristie , Kulyk Danielle , Vanschaik Allison , Daisey Sierra , Rojas Catherine , Wiersielis Kimberly , Yasrebi Ali , Degroat Thomas J. , Sun Yuxiang , Roepke Troy A.
Published: September 2022

The gut peptide, ghrelin, mediates energy homeostasis and reproduction by acting through its receptor, growth hormone secretagogue receptor (GHSR), expressed in hypothalamic neurons in the arcuate (ARC). We have shown 17β-estradiol (E2) increases Ghsr expression in Kisspeptin/Neurokinin B/Dynorphin (KNDy) neurons, enhancing sensitivity to ghrelin. We hypothesized that E2-induced Ghsr expression augments KNDy sensitivity in a fasting state by elevating ghrelin to disrupt energy expenditure in females. We produced a Kiss1-GHSR knockout to determine the role of GHSR in ARC KNDy neurons. We found that changes in ARC gene expression with estradiol benzoate (EB) treatment were abrogated by the deletion of GHSR and ghrelin abolished these differences. We also observed changes in metabolism and fasting glucose levels. Additionally, knockouts were resistant to body weight gain on a high fat diet (HFD). Behaviorally, we found that knockouts on HFD exhibited reduced anxiety-like behavior. Furthermore, knockouts did not refeed to the same extent as controls after a 24 h fast. Finally, in response to cold stress, knockout females had elevated metabolic parameters compared to controls. These data indicate GHSR in Kiss1 neurons modulate ARC gene expression, metabolism, glucose homeostasis, behavior, and thermoregulation, illustrating a novel mechanism for E2 and ghrelin to control Kiss1 neurons.
Effect of Voluntary Wheel-Running Exercise on the Endocrine and Inflammatory Response to Social Stress: Conditioned Rewarding Effects of Cocaine
Authors: Ferrer-Pérez Carmen , Reguilón Marina D. , Miñarro José , Rodríguez-Arias Marta
Published: September 2022

The present paper evaluates the effect of physical activity on the increase of the conditioned rewarding effects of cocaine induced by intermittent social stress and on the neuroinflammatory response that contributes to the enhancement of drug response. For that purpose, three studies were designed in which social stress was induced in different samples of mice through a social-defeat protocol; the mice underwent an increase of physical activity by different modalities of voluntary wheel running (continuous and intermittent access). The results showed that continuous access to running wheels prior to stress enhanced the establishment of cocaine place preference, whereas an intermittent access exerted a protective effect. Wheel running contingent to cocaine administration prevented the development of conditioned preference, and if applied during the extinction of drug memories, it exerted a dual effect depending on the stress background of the animal. Our biological analysis revealed that increased sensitivity to cocaine may be related to the fact that wheel running promotes inflammation though the increase of IL-6 and BDNF levels. Together, these results highlight that physical exercise deeply impacts the organism’s response to stress and cocaine, and these effects should be taken into consideration in the design of a physical intervention.
Ultrasound-Based Hepatic Elastography in Non-Alcoholic Fatty Liver Disease: Focus on Patients with Type 2 Diabetes
Authors: Cazac Georgiana-Diana , Lăcătușu Cristina-Mihaela , Mihai Cătălina , Grigorescu Elena-Daniela , Onofriescu Alina , Mihai Bogdan-Mircea
Published: September 2022

Non-alcoholic fatty liver disease (NAFLD) is the most prevalent liver disease and is the hepatic expression of metabolic syndrome. The development of non-invasive methods for the diagnosis of hepatic steatosis and advanced fibrosis in high-risk patients, especially those with type 2 diabetes mellitus, is highly needed to replace the invasive method of liver biopsy. Elastographic methods can bring significant added value to screening and diagnostic procedures for NAFLD in patients with diabetes, thus contributing to improved NAFLD management. Pharmacological development and forthcoming therapeutic measures that address NAFLD should also be based on new, non-invasive, and reliable tools that assess NAFLD in at-risk patients and be able to properly guide treatment in individuals with both diabetes and NAFLD. This is the first review aiming to outline and discuss recent studies on ultrasound-based hepatic elastography, focusing on NAFLD assessment in patients with diabetes.
Postnatal Conditional Deletion of Bcl11b in Striatal Projection Neurons Mimics the Transcriptional Signature of Huntington’s Disease
Authors: Song Sicheng , Creus Muncunill Jordi , Galicia Aguirre Carlos , Tshilenge Kizito-Tshitoko , Hamilton B. Wade , Gerencser Akos A. , Benlhabib Houda , Cirnaru Maria-Daniela , Leid Mark , Mooney Sean D. , Ellerby Lisa M. , Ehrlich Michelle E.
Published: September 2022

The dysregulation of striatal gene expression and function is linked to multiple diseases, including Huntington’s disease (HD), Parkinson’s disease, X-linked dystonia-parkinsonism (XDP), addiction, autism, and schizophrenia. Striatal medium spiny neurons (MSNs) make up 90% of the neurons in the striatum and are critical to motor control. The transcription factor, Bcl11b (also known as Ctip2), is required for striatal development, but the function of Bcl11b in adult MSNs in vivo has not been investigated. We conditionally deleted Bcl11b specifically in postnatal MSNs and performed a transcriptomic and behavioral analysis on these mice. Multiple enrichment analyses showed that the D9-Cre-Bcl11btm1.1Leid transcriptional profile was similar to the HD gene expression in mouse and human data sets. A Gene Ontology enrichment analysis linked D9-Cre-Bcl11btm1.1Leid to calcium, synapse organization, specifically including the dopaminergic synapse, protein dephosphorylation, and HDAC-signaling, commonly dysregulated pathways in HD. D9-Cre-Bcl11btm1.1Leid mice had decreased DARPP-32/Ppp1r1b in MSNs and behavioral deficits, demonstrating the dysregulation of a subtype of the dopamine D2 receptor expressing MSNs. Finally, in human HD isogenic MSNs, the mislocalization of BCL11B into nuclear aggregates points to a mechanism for BCL11B loss of function in HD. Our results suggest that BCL11B is important for the function and maintenance of mature MSNs and Bcl11b loss of function drives, in part, the transcriptomic and functional changes in HD.
Perhexiline Therapy in Patients with Type 2 Diabetes: Incremental Insulin Resistance despite Potentiation of Nitric Oxide Signaling
Authors: Chong Cher-Rin , Liu Saifei , Imam Hasan , Heresztyn Tamila , Sallustio Benedetta C. , Chirkov Yuliy Y. , Horowitz John D.
Published: September 2022

Perhexiline (Px) inhibits carnitine palmitoyltransferase 1 (CPT1), which controls uptake of long chain fatty acids into mitochondria. However, occasional cases of hypoglycaemia have been reported in Px-treated patients, raising the possibility that Px may also increase sensitivity to insulin. Furthermore, Px increases anti-aggregatory responses to nitric oxide (NO), an effect which may theoretically parallel insulin sensitization. We therefore sought to examine these relationships in patients with stable Type 2 diabetes (T2D) and cardiovascular disease (n = 30). Px was initiated, and dosage was titrated, to reach the therapeutic range and thus prevent toxicity. Investigations were performed before and after 2 weeks, to examine changes in insulin sensitivity and, utilizing aggregometry in whole blood, platelet responsiveness to the anti-aggregatory effects of the NO donor sodium nitroprusside (SNP). Other parameters that affect may affect NO signalling were also evaluated. Px substantially potentiated inhibition of platelet aggregation by SNP (from 16.7 ± 3.0 to 27.3 ± 3.7%; p = 0.005). Px did not change fasting blood glucose concentrations but reduced insulin sensitivity (HOMA-IR score increased from median of 4.47 to 6.08; p = 0.028), and increased fasting plasma insulin concentrations (median 16.5 to 19.0 mU/L; p = 0.014). Increases in SNP responses tended (r = −0.30; p = 0.11) to be reciprocally related to increases in HOMA-IR, and increases in HOMA-IR were greater (p = 0.002) in patients without NO-sensitizing effects. No patient developed symptomatic hypoglycaemia, nor was there any other short-term toxicity of Px. Thus, in patients with stable T2D and cardiovascular disease, Px increases anti-aggregatory responsiveness to NO, but is not an insulin sensitizer, and does not induce hypoglycaemia. Absence of NO-sensitizing effect occurs in approximately 30% of Px-treated patients with T2D, and is associated with induction of insulin resistance in these patients.
The SIRT2 Pathway Is Involved in the Antiproliferative Effect of Flavanones in Human Leukemia Monocytic THP-1 Cells
Authors: Russo Caterina , Maugeri Alessandro , De Luca Laura , Gitto Rosaria , Lombardo Giovanni Enrico , Musumeci Laura , De Sarro Giovambattista , Cirmi Santa , Navarra Michele
Published: September 2022

Acute myeloid leukemia (AML) represents the most alarming hematological disease for adults. Several genetic modifications are known to be pivotal in AML; however, SIRT2 over-expression has attracted the scientific community’s attention as an unfavorable prognostic marker. The plant kingdom is a treasure trove of bioactive principles, with flavonoids standing out among the others. On this line, the aim of this study was to investigate the anti-leukemic properties of the main flavanones of Citrus spp., exploring the potential implication of SIRT2. Naringenin (NAR), hesperetin (HSP), naringin (NRG), and neohesperidin (NHP) inhibited SIRT2 activity in the isolated recombinant enzyme, and more, the combination between NAR and HSP. In monocytic leukemic THP-1 cells, only NAR and HSP induced antiproliferative effects, altering the cell cycle. These effects may be ascribed to SIRT2 inhibition since these flavonoids reduced its gene expression and hampered the deacetylation of p53, known sirtuin substrate, and contextually modulated the expression of the downstream cell cycle regulators p21 and cyclin E1. Additionally, these two flavanones proved to interact with the SIRT2 inhibitory site, as shown by docking simulations. Our results suggest that both NAR and HSP may act as anti-leukemic agents, alone and in combination, via targeting the SIRT2/p53/p21/cyclin E1 pathway, thus encouraging deeper investigations.
Omics and Multi-Omics Analysis for the Early Identification and Improved Outcome of Patients with Psoriatic Arthritis
Authors: Gurke Robert , Bendes Annika , Bowes John , Koehm Michaela , Twyman Richard M. , Barton Anne , Elewaut Dirk , Goodyear Carl , Hahnefeld Lisa , Hillenbrand Rainer , Hunter Ewan , Ibberson Mark , Ioannidis Vassilios , Kugler Sabine , Lories Rik J. , Resch Eduard , Rüping Stefan , Scholich Klaus , Schwenk Jochen M. , Waddington James C. , Whitfield Phil , Geisslinger Gerd , FitzGerald Oliver , Behrens Frank , Pennington Stephen R.
Published: September 2022

The definitive diagnosis and early treatment of many immune-mediated inflammatory diseases (IMIDs) is hindered by variable and overlapping clinical manifestations. Psoriatic arthritis (PsA), which develops in ~30% of people with psoriasis, is a key example. This mixed-pattern IMID is apparent in entheseal and synovial musculoskeletal structures, but a definitive diagnosis often can only be made by clinical experts or when an extensive progressive disease state is apparent. As with other IMIDs, the detection of multimodal molecular biomarkers offers some hope for the early diagnosis of PsA and the initiation of effective management and treatment strategies. However, specific biomarkers are not yet available for PsA. The assessment of new markers by genomic and epigenomic profiling, or the analysis of blood and synovial fluid/tissue samples using proteomics, metabolomics and lipidomics, provides hope that complex molecular biomarker profiles could be developed to diagnose PsA. Importantly, the integration of these markers with high-throughput histology, imaging and standardized clinical assessment data provides an important opportunity to develop molecular profiles that could improve the diagnosis of PsA, predict its occurrence in cohorts of individuals with psoriasis, differentiate PsA from other IMIDs, and improve therapeutic responses. In this review, we consider the technologies that are currently deployed in the EU IMI2 project HIPPOCRATES to define biomarker profiles specific for PsA and discuss the advantages of combining multi-omics data to improve the outcome of PsA patients.
Mineral-Enriched Postbiotics: A New Perspective for Microbial Therapy to Prevent and Treat Gut Dysbiosis
Authors: Dinu Laura-Dorina , Avram Ionela , Pelinescu Diana-Roxana , Vamanu Emanuel
Published: September 2022

Postbiotics are non-viable probiotic preparations that confer a health benefit on the host. In the last years, scientific literature has proved that postbiotics have health-promoting features and technological advantages compared to probiotics, augmenting their full potential application in the food and pharmaceutical industries. The current work comprehensively summarizes the benefits and potential applications of postbiotics and essential mineral-enriched biomass and proposes a new strategy for microbial therapy—mineral-enriched postbiotics. We hypothesize and critically review the relationship between micronutrients (calcium, magnesium, iron, zinc, selenium) and postbiotics with gut microbiota, which has been barely explored yet, and how the new approach could be involved in the gut microbiome modulation to prevent and treat gut dysbiosis. Additionally, the bioactive molecules and minerals from postbiotics could influence the host mineral status, directly or through gut microbiota, which increases the mineral bioavailability. The review increases our understanding of the health improvements of mineral-enriched postbiotics, including antioxidant functions, highlighting their perspective on microbial therapy to prevent and threaten gut-related diseases.
Relationship between Glutathione-Dependent Enzymes and the Immunohistochemical Profile of Glial Neoplasms
Authors: Obukhova Larisa , Kopytova Tatiana , Murach Elena , Shchelchkova Natalya , Kontorshchikova Claudia , Medyanik Igor , Orlinskaya Natalia , Grishin Artem , Kontorshchikov Michael , Badanina Dariya
Published: September 2022

This research aimed to investigate the relationships between the parameters of glutathione metabolism and the immunohistochemical characteristics of glial tumors. Postoperative material from 20 patients with gliomas of different grades of anaplasia was analyzed. Bioinformatic analysis of the interactions between the gliomas’ immunohistochemical markers and their glutathione-dependent enzymes was carried out using the STRING, BioGrid, while Signor databases revealed interactions between such glioma markers as IDH and p53 and the glutathione exchange enzymes (glutathione peroxidase, glutathione reductase, glutathione S-transferase). The most pronounced relationship with glutathione metabolism was demonstrated by the level of the nuclear protein Ki67 as a marker of proliferative activity, and the presence of the IDH1 mutation as one of the key genetic events of gliomagenesis. The glutathione system is an active participant in the body’s antioxidant defense, involving the p53 markers and MGMT promoter methylation. It allows characterization of the gliomal cells’ status at different stages of tumor development.
A Single Session of Bifrontal tDCS Can Improve Facial Emotion Recognition in Major Depressive Disorder: An Exploratory Pilot Study
Authors: Imbert Laetitia , Moirand Rémi , Bediou Benoit , Koenig Olivier , Chesnoy Gabrielle , Fakra Eric , Brunelin Jérôme
Published: September 2022

Emotional processing deficits are key features in major depressive disorder (MDD). Neuroimaging studies indicate that the dorsolateral prefrontal cortex (DLPFC) plays a pivotal role in both depressive symptoms and emotional processing. Recently, transcranial Direct Current Stimulations (tDCS) applied over the DLPFCs have held the promise to alleviate the symptoms in patients with MDD, but the effect on emotional processing in the patients is unclear. Here, we investigated the effect of a single session of tDCS over the DLPFCs on the emotional processing in patients with treatment-resistant MDD. In a randomized sham-controlled study, 35 patients received a single 30 min session of either active (2 mA, n = 18) or sham tDCS (n = 17). The anode was placed over the left and the cathode over the right DLPFC. Emotional processing accuracy was measured by a facial emotion recognition (FER) task. We observed an overall improvement in FER performance after the active tDCS, but not the sham tDCS. These exploratory results suggest that a single session of tDCS over the DLPFCs may improve FER in MDD, a crucial function of social cognition. Further studies are needed to investigate whether this acute improvement of FER in response to a single tDCS session could translate into clinical benefits or predict remission following repeated sessions of stimulation.
Calcium Carbonate Mineralization of Microalgae
Authors: Natsi Panagiota D. , Koutsoukos Petros G.
Published: September 2022

Biological substrates catalyze the nucleation and growth of sparingly soluble salts however, the underlying mechanism is largely unknown. In the present study, the growth of calcium carbonate (CaCO3), on Acutodesmus obliquus (AO) microalgae was investigated. The test microalgae favored the growth of CaCO3 from solutions supersaturated with respect to calcite (7.94 < SRcalcite < 104.71). The precipitation of calcite on AO was not preceded by measurable induction times, and the rates of calcite crystal growth were higher for higher microalgae cell concentrations. The presence of the microalgae cultivation medium and illumination of the supersaturated solutions accelerated the precipitation of CaCO3, increasing the rate by 75% in comparison with the respective value in its absence. AO cultures, air dried at 25 °C yielded higher precipitation rates, in comparison with the respective rates in the presence of active AO cultures. At 70 °C, nucleation and growth were suppressed, due to the destruction of the molecular structure of the microalgae. The CaCO3 precipitation rates on calcite precipitated on air-dried AO culture, were doubled in comparison with the respective rates obtained with the respective quantities of each component of the composite substrate.
Effect of α-Amylase on the Structure of Chia Seed Mucilage
Authors: Piazza Francesco , Colella Matilde , Cinelli Giuseppe , Lopez Francesco , Donati Ivan , Sacco Pasquale
Published: September 2022

Thanks to its nutritional and mechanical properties, chia seed mucilage is becoming increasingly popular in the food industry as a small biomolecule. The mechanical properties of an ingredient are a key element for food appreciation during chewing. Therefore, with this study, we explore for the first time the structural changes that chia seed mucilage undergoes when treated with α-amylase, the most abundant enzyme in human saliva. First, rheological time-sweep tests were performed on samples with different enzyme and constant chia mucilage concentrations. Then, the effect of increasing the chia mucilage concentration at a constant enzyme content was investigated. The results show that structural changes occur after enzyme treatment. Rheological measurements show a thickening of the material with an increase in the elastic modulus depending on the concentrations of α-amylase and chia used. This effect is attributed to the release and aggregation of insoluble fibrous aggregates that naturally form the mucilage after the cleavage of the α-1,4-glucoside bond between the α-D-glucopyranose residue and the second β-D-xylopyranose residue by α-amylase. Thus, our data suggest an α-amylase-mediated restructuring of the chia mucilage network that could have implications for the commercial processing of this material.
Design of the Jump Mechanism for a Biomimetic Robotic Frog
Authors: Fan Jizhuang , Du Qilong , Dong Zhihui , Zhao Jie , Xu Tian
Published: September 2022

Frogs are vertebrate amphibians with both efficient swimming and jumping abilities due to their well-developed hind legs. They can jump over obstacles that are many or even tens of times their size on land. However, most of the current jumping mechanisms of biomimetic robotic frogs use simple four-bar linkage mechanisms, which has an unsatisfactory biomimetic effect on the appearance and movement characteristics of frogs. At the same time, multi-joint jumping robots with biomimetic characteristics are subject to high drive power requirements for jumping action. In this paper, a novel jumping mechanism of a biomimetic robotic frog is proposed. Firstly, the structural design of the forelimb and hindlimb of the frog is given, and the hindlimb of the robotic frog is optimized based on the design of a single-degree-of-freedom six-bar linkage. A simplified model is established to simulate the jumping motion. Secondly, a spring energy storage and trigger mechanism is designed, including incomplete gear, one-way bearing, torsion spring, and so on, to realize the complete jumping function of the robot, that is, elastic energy storage and regulation, elastic energy release, and rapid leg retraction. Thirdly, the experimental prototype of the biomimetic robotic frog is fabricated. Finally, the rationality and feasibility of the jumping mechanism are verified by a jumping experiment. This work provides a technical and theoretical basis for the design and development of a high-performance amphibious biomimetic robotic frog.
Metabolic Pathways in Breast Cancer Reprograming: An Insight to Non-Coding RNAs
Authors: Abedi-Gaballu Fereydoon , Kamal Kazemi Elham , Salehzadeh Seyed Ahmad , Mansoori Behnaz , Eslami Farhad , Emami Ali , Dehghan Gholamreza , Baradaran Behzad , Mansoori Behzad , Cho William C.
Published: September 2022

Cancer cells reprogram their metabolisms to achieve high energetic requirements and produce precursors that facilitate uncontrolled cell proliferation. Metabolic reprograming involves not only the dysregulation in glucose-metabolizing regulatory enzymes, but also the enzymes engaging in the lipid and amino acid metabolisms. Nevertheless, the underlying regulatory mechanisms of reprograming are not fully understood. Non-coding RNAs (ncRNAs) as functional RNA molecules cannot translate into proteins, but they do play a regulatory role in gene expression. Moreover, ncRNAs have been demonstrated to be implicated in the metabolic modulations in breast cancer (BC) by regulating the metabolic-related enzymes. Here, we will focus on the regulatory involvement of ncRNAs (microRNA, circular RNA and long ncRNA) in BC metabolism, including glucose, lipid and glutamine metabolism. Investigation of this aspect may not only alter the approaches of BC diagnosis and prognosis, but may also open a new avenue in using ncRNA-based therapeutics for BC treatment by targeting different metabolic pathways.
Au-Decorated Polyaniline-ZnO Electrospun Composite Nanofiber Gas Sensors with Enhanced Response to NO2 Gas
Authors: Bonyani Maryam , Zebarjad Seyed Mojtaba , Janghorban Kamal , Kim Jin-Young , Kim Hyoun Woo , Kim Sang Sub
Published: September 2022

Ternary systems are less studied for sensing applications due to complex synthesis procedures. However, they have more sources of resistance modulation, leading to an enhanced gas response. In this study, a ternary system, namely Au-decorated ZnO-polyaniline (PANI) composite nanofibers with different amounts of PANI (10, 25, and 50 wt.%) were synthesized for NO2 gas sensing studies. First, ZnO nanofibers were synthesized by electrospinning, and then an Au layer (9 nm) was coated on the ZnO nanofibers. Finally, PANI was coated onto the prepared Au-decorated ZnO nanofibers. NO2 gas sensing investigations indicated that the sensor with 25 wt.% PANI had the best response to NO2 gas at 300 °C. In addition, the optimized sensor exhibited high selectivity to NO2 gas. The improved performance of the optimal gas sensor was attributed to the role of Au, the formation of ZnO-PANI heterojunctions, and the optimal amount of PANI. The promising effect of this ternary system for NO2 sensing was demonstrated, and it can be extended to other similar systems.
Lung Ultrasound and Neutrophil Lymphocyte Ratio in Early Diagnosis and Differentiation between Viral and Bacterial Pneumonia in Young Children
Authors: Omran Ahmed , Awad Heba , Ibrahim Mostafa , El-Sharkawy Sonya , Elfiky Samar , Rezk Ahmed R.
Published: September 2022

Lung ultrasound (LUS) is a crucial diagnostic tool for identifying pneumonia in the pediatric age group. However, it plays a limited role in the early distinction between viral and bacterial pneumonia in children. The objectives of our study were to determine if LUS and the neutrophil-lymphocyte ratio (NLR) were useful in identifying and distinguishing between viral and bacterial pneumonia in Egyptian children under the age of two. Within the first 12 h of being admitted to our department, 52 children with clinical symptoms and signs suggestive of community-acquired pneumonia (CAP) underwent LUS and the NLR. LUS and the NLR strongly differentiated children with viral from those with bacterial pneumonia. For the early diagnosis and differentiation between viral and bacterial pneumonia in young Egyptian children, LUS was proven to be a noninvasive and reliable method. Combining the NLR with LUS increased the diagnostic accuracy when evaluating children suspected of having pneumonia.
The Effect of Leisure Time Sport on Executive Functions in Danish 1st Grade Children
Authors: Siersbaek Gitte Meilandt , Have Mona , Wedderkopp Niels
Published: September 2022

Physical inactivity can influence children’s executive functions with severe impact on wellbeing and academic learning. The objective is to study the effect of leisure time sport on executive functions in Danish 1st grade children, and secondary to explore if socio-economy is a confounder for associations between leisure time sport and executive functions. This study is a sub-study nested within a cluster-randomized controlled trial with two arms (ClinicalTrials.gov, NCT02488460). 505 children from twelve schools, mean age 7.2 ± 0.3 years participated. Outcomes for executive function were “Modified Eriksen Flanker/Reverse Flanker Task” and “Behavior Rating Inventory of Executive Function” (BRIEF-P). Parents used SMS-tracking to register their children’s leisure time sport. Multivariate analyzes was performed using mixed linear regression, with adjustment for highest parental education, sex, municipality, and school-type. We found that leisure time sport seems to significantly improve working memory (WM) with nearly 20%, and furthermore it seems to be a significant predictor of ‘Initiate’ (the ability to begin an activity, to generate ideas, responses or problem-solving strategies). Socio-economy was not found to be a confounder. This study lends support to the hypothesis that leisure time sport is related to working memory capacity in children.
Nasal Cannula with Long and Narrow Tubing for Non-Invasive Respiratory Support in Preterm Neonates: A Systematic Review and Meta-Analysis
Authors: Anand Pratima , Kaushal Monika , Ramaswamy Viraraghavan Vadakkencherry , Pullattayil S. Abdul Kareem , Razak Abdul , Trevisanuto Daniele
Published: September 2022

Background: Cannulas with long and narrow tubing (CLNT) are increasingly being used as an interface for noninvasive respiratory support (NRS) in preterm neonates; however, their efficacy compared to commonly used nasal interfaces such as short binasal prongs (SBP) and nasal masks (NM) has not been widely studied. Material and Methods: Medline, Embase, CENTRAL, Health Technology Assessment Database, and Web of Science were searched for randomized clinical trials (RCTs) and observational studies investigating the efficacy of CLNT compared to SBP or NM in preterm neonates requiring NRS for primary respiratory and post-extubation support. A random-effects meta-analysis was used for data synthesis. Results: Three RCTs and three observational studies were included. Clinical benefit or harm could not be ruled out for the outcome of need for invasive mechanical ventilation (IMV) for CLNT versus SBP or NM [relative risk (RR) 1.37, 95% confidence interval (CI) 0.61–3.04, certainty of evidence (CoE) low]. The results were also inconclusive for the outcome of treatment failure [RR 1.20, 95% CI 0.48–3.01, CoE very low]. Oropharyngeal pressure transmission was possibly lower with CLNT compared to other interfaces [MD −1.84 cm H20, 95% CI −3.12 to −0.56, CoE very low]. Clinical benefit or harm could not be excluded with CLNT compared to SBP or NM for the outcomes of duration of IMV, nasal trauma, receipt of surfactant, air leak, and NRS duration. Conclusion: Very low to low CoE and statistically nonsignificant results for the clinical outcomes precluded us from making any reasonable conclusions; however, the use of CLNT as an NRS interface, compared to SBP or NM, possibly transmits lower oropharyngeal pressures. We suggest adequately powered multicentric RCTs to evaluate the efficacy of CLNT when compared to other interfaces.
Postoperative Epileptic Seizures in Children
Authors: Massimi Luca , Frassanito Paolo , Bianchi Federico , Fiorillo Luigi , Battaglia Domenica Immacolata , Tamburrini Gianpiero
Published: September 2022

Background: Postoperative seizures (PS) occur in 10–15% of patients. This study aims to provide an update on the role of surgery in PS. Methods: All children undergoing a craniotomy for supratentorial lesions in the last 10 years were considered except those with preoperative seizures, perioperative antiepileptic drugs prophylaxis, head-injury and infections, repeated surgery, or preoperative hyponatremia. Children undergoing surgery for intra-axial lesions (Group 1, 74 cases) were compared with those harboring extra-axial lesions (Group 2, 91 cases). Results: PS occurred in 9% of 165 cases and epilepsy in 3% of 165 cases (mean follow-up: 5.7 years). There was no difference between the two study groups with regard to demographic data or tumor size. Group 1 showed a higher rate of gross total tumor resection (p = 0.002), while Group 2 had a higher rate of postoperative hyponatremia (p < 0.0001). There were no differences between the two groups in the occurrence of seizures (6.7% vs. 11%) or epilepsy (2.7% vs. 3.2%). No correlations were found between seizures and age, tumor location, histotype, tumor size, or the extent of tumor resection. Hyponatremia affected the risk of PS in Group 2 (p = 0.02). Conclusions: This study shows a lower rate of PS and epilepsy than series including children with preoperative seizures. Hyponatremia has a significant role. Neurosurgery is safe but surgical complications may cause late epilepsy.
Relationship Analogy between Sleep Bruxism and Temporomandibular Disorders in Children: A Narrative Review
Authors: Lee Yeon-Hee
Published: September 2022

Sleep bruxism (SB) is a condition characterized by repetitive clenching or grinding teeth and/or by bracing or thrusting of the mandible during sleep. Although SB is not considered a disorder in children, SB can be a potential physical and psychological hazard or consequence, and this study examines whether SB is a risk factor for TMD as it is in adults. A narrative review on the topic of inferring the relationship between sleep bruxism and TMD in children was performed based on a search in the PubMed and Google Scholar databases for articles published between 1999 and 2022. A total of 76 articles were included in this review. SB is very common in children, may be related to psychological distress or sleep breathing disorder, with a prevalence of up to 49%, and mainly occurs in the non-rapid eye movement stage in the sleep structure. SB may be one cause of TMD. The prevalence of TMD in children is 16–33%. Compared to the female-dominant TMD prevalence in adults, the sex-differences in TMD prevalence in children are less pronounced. However, TMD-related pain is more prevalent in girls than in boys. Given the complex etiology of each of SB and TMD in children, it can be inferred that the explanation of the relationship between the two conditions is very challenging. Ultimately, their relationship should be understood in the individual biopsychosocial model in the process of special physical growth and mental development of children. Moreover, appropriate clinical guidelines for a definitive diagnosis of SB and TMD in children and more research with a high scientific evidence level, which is comprehensive, considering physical, psychological, genetic, and social cultural factors, are required.
Clinical Features and Management of Urethral Foreign Bodies in Children: A 10-Year Retrospective Study
Authors: Kong Xiangpan , Wei Chun
Published: September 2022

(1) Background: Urethral foreign bodies (UFBs) are very rare in children, and their treatment remains challenging. (2) Methods: A retrospective analysis was performed on 40 patients who were admitted to our hospital due to UFBs from June 2011 to June 2021. The clinical features and treatment experiences of these children are summarized. (3) Results: A total of 40 children were enrolled in the study, 17 boys and 23 girls. A majority of the boys (median age: 11.8 years) were of puberal age, and the main cause of the UFBs was sexual gratification (94.1%). Girls were almost always in early childhood (median age: 1.8 years), and most of the UFBs were related to specific clothing in specific regions and seasons. Ultrasound had a high accuracy in the diagnosis of female UFBs; the sensitivity and specificity were 88.9% and 85.7%, respectively. Most UFBs could be removed using a cystoscope (82.4% in boys, 100% in girls). All the children had a good prognosis and no complications occurred during follow-up. (4) Conclusions: Ultrasound is a reliable and sensitive method for the diagnosis of UFBs in girls. Cystoscopy is a reliable surgical method for UFBs.
Vojta Therapy Affects Trunk Control and Postural Sway in Children with Central Hypotonia: A Randomized Controlled Trial
Authors: Ha Sun-Young , Sung Yun-Hee
Published: September 2022

(1) Background: Decreased trunk stability is accompanied by delay in motor development in children with central hypotonia. We investigated the effect of Vojta therapy on trunk control in the sitting position in children with central hypotonia. (2) Methods: In 20 children with central hypotonia, Vojta therapy was applied to the experimental group (n = 10) and general physical therapy to the control group (n = 10). The intervention was applied for 30 min per session, three times a week, for a total of six weeks. We assessed abdominal muscle thickness, trunk control (segmental assessment of trunk control), trunk angle and trunk sway in a sitting position, and gross motor function measure-88. (3) Results: In the experimental group, the thicknesses of internal oblique and transversus abdominis were significantly increased (p < 0.05). The segmental assessment of trunk control score was significantly increased (p < 0.05), and the trunk sway significantly decreased (p < 0.05). Gross motor function measure-88 was significantly increased (p < 0.05). (4) Conclusions: Vojta therapy can be suggested as an effective intervention method for improving trunk control and gross motor function in children with central hypotonia.
Intensity, Duration and Spatial Coverage of Aridity during Meteorological Drought Years over Northeast Thailand
Authors: Dlamini Tenanile , Songsom Veeranun , Koedsin Werapong , Ritchie Raymond J.
Published: September 2022

Gaps in drought monitoring result in insufficient preparation measures for vulnerable areas. This paper employed the standardized precipitation index (SPI) to identify meteorological drought years and the Thornthwaite aridity index (TAI) to evaluate aridity in three provinces of northeast Thailand growing cassava and sugarcane at massive scales. Precipitation and temperature data were sourced from Global Land Data Assimilation System-2 (GLDAS-2) Noah Model products at 0.25 degree resolution and used for calculating the drought indices. This study was conducted for the period of 2004 to 2015. The SPI was computed for 1, 3 and 6 months scales to measure short- to medium-term moisture. The results indicated major meteorological drought years as 2004, 2005, 2010, 2012, 2014 and 2015. A range of 1 to 3 months of extreme rainfall shortage was experienced during each of these years, including the growing season of 2004, 2012 and 2015. TAI-based results indicated that the area experiences an average of 7 to 8 months of aridity during drought periods, compared to the historical overall average of 6 months. The spatial TAI for the major drought years indicated delayed onset, intermittency or early cut-off of the rainy season. The year 2004 was the most intense in terms of aridity. The longest duration of aridness for some areas was between 9 and 10 months in 2012 and 2014, respectively. In terms of spatial coverage, all meteorological drought years had out-of-season aridity. Based on the region’s historical records, this highlighted an increase in the frequency of droughts and duration of aridity. A disturbance in the growing season has the potential to affect crop yields, hence, the need to improve and strengthen existing adaptive measures for agriculture as the main source of food and income in the northeast.
On the Intercontinental Transferability of Regional Climate Model Response to Severe Forestation
Authors: Asselin Olivier , Leduc Martin , Paquin Dominique , Di Luca Alejandro , Winger Katja , Bukovsky Melissa , Music Biljana , Giguère Michel
Published: September 2022

The biogeophysical effects of severe forestation are quantified using a new ensemble of regional climate simulations over North America and Europe. Following the protocol outlined for the Land-Use and Climate Across Scales (LUCAS) intercomparison project, two sets of simulations are compared, FOREST and GRASS, which respectively represent worlds where all vegetation is replaced by trees and grasses. Three regional climate models were run over North America. One of them, the Canadian Regional Climate Model (CRCM5), was also run over Europe in an attempt to bridge results with the original LUCAS ensemble, which was confined to Europe. Overall, the CRCM5 response to forestation reveals strong inter-continental similarities, including a pronounced wintertime and springtime warming concentrated over snow-masking evergreen forests. Crucially, these northern evergreen needleleaf forests populate lower, hence sunnier, latitudes in North America than in Europe. Snow masking reduces albedo similarly over both continents, but stronger insolation amplifies the net shortwave radiation and hence warming simulated over North America. In the summertime, CRCM5 produces a mixed response to forestation, with warming over northern needleleaf forests and cooling over southern broadleaf forests. The partitioning of the turbulent heat fluxes plays a major role in determining this response, but it is not robust across models over North America. Implications for the inter-continental transferability of the original LUCAS results are discussed.
Exploring the Colors of Copper-Containing Pigments, Copper (II) Oxide and Malachite, and Their Origins in Ceramic Glazes
Authors: Peng Iris , Hills-Kimball Katie , Lovelace Isabela Miñana , Wang Junyu , Rios Matthew , Chen Ou , Wang Li-Qiong
Published: September 2022

The colors of copper-containing pigments, copper (II) oxide and malachite, and their origins in ceramic glazes were systematically examined over a wide firing temperature range using a suite of analytical and spectroscopy techniques including SEM, UV-Vis FORS, XRD, FTIR, and EPR to gain new insight into the structural and chemical transformations of the glaze during firing. The two colorants investigated were black copper (II) oxide (CuO) nanopowder and blue-green basic copper carbonate, or malachite (Cu2CO3(OH)2), both of which produce a final light blue color following firing. Additionally, silicon carbide (SiC) was used to locally reduce CuO to simulate firing glazes in a reductive environment and produce a final red color. At lower temperatures, malachite was found to decompose to form CuO at 550 °C, elucidating the reason that two different copper colorants could be used interchangeably to form the same “Robin’s Egg Blue” color. At 850 °C, a glaze sintering process occurred, resulting in the distribution of Cu2+ in a square planar geometry and an observed blue color. This structural change occurred at temperatures lower than the glaze’s melting point, indicating that complete vitrification of the glaze is not required for glaze coloration. Conversely, the reduction in Cu2+ to Cu+ through the addition of SiC did not occur until the glaze was fired above the melting temperature (1000 °C), signifying that high temperatures are required for the redox reaction to occur. This study sheds light on intermediate colorant-glaze interactions that are beneficial for understanding and predicting glaze coloring upon exposure to varying temperatures, and the results from this study can be applied to better-controlled glaze production for artists and a deeper appreciation of ceramic glaze chemistry and aesthetics.