bluuebunny/crossref_metadata_2025 huggingface dataset:
Obtaining and Characteristics of Plant Cell Cultures of Аjuga turkestanica (Rgl.) - Producing of Phytoexdystroides
Authors: Sobolkova G.I. , Kharitonov T.D. , Nigmatullaev A.M. , Sagdullaev Sh.Sh. , Kochkin D.V. , Nosov A.M.
Published: July 2019

Callus cultures of Ajuga turkestanica (Rgl.) Briq. (Lamiaceae) were obtained from a wild plant, and the processes of morphogenesis in these cultures were studied. Depending on the hormonal composition of media, it is possible to obtain gemmogenesis or rhizogenesis in the first 6 to 8 growth cycles; after 10-15 growth cycles, the ability of morphogenesis was lost. Suspension cell cultures were initiated from some well-growing calluses. As a result, about one hundred calluses and suspension cell lines were obtained from the Ajuga turkestanica plant cells, a number of which were characterized by intensive growth and were analyzed by the HPLC-MS method for the presence of phytoecdysteroids. In most of the investigated lines, 20-hydroxyecdysone and turkesterone were found, the content of the first being 30-50 times higher than the second (in the most productive lines, 2.0-2.5 mg/g and 0.04-0.05 mg/g dry weight, respectively). An increase in the content of phytoecdysteroids in the in vitro cultivated cells by the end of the growing cycle was observed. However, phytoecdysteroids were not found in many of the obtained cell lines. Further research is needed to clarify the reasons for the presence or absence of phytoecdysteroids in Ajuga turkestanica plant cell cultures.

Ajuga turkestanica, plant cell culture, growth regulators, differentiation, gemmogenesis, rhizogenesis, phytoecdysteroids, 20-hydroxy ecdysone, turkesterone

The work was financially supported by the Russian Science Foundation (№ 16-14-00126).
Method for Obtaining Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor
Authors: Lebedev L.R. , Volosnikova Е.А. , Gileva I.P. , Gogina Ya.S. , Tereshchenko Т.А. , Kochneva G.V. , Grazhdantseva A.A. , Danilenko E.D.
Published: July 2019

A suggested method for obtaining recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) includes the accumulation of the producer strain biomass enriched with the target product up to 30% of total protein content, its isolation and purification. The later consists of the following stages: ultrasound cell disintegration, washing of inclusion bodies with buffer solutions, GM-CSF solubilization from inclusion bodies by 6 M urea, denaturation-renaturation of protein molecules and purification by chromatography on DEAE-Sepharose and combined chromatography on CM-Sepharose and Q-Sepharose followed by dialysis. The proposed method makes it possible to yield up to 10 mg of the protein preparation from 1 g of wet cells with the purity of 98% and high activity shown on the human erythroleukemia cell line.

granulocyte-macrophage colony-stimulating, GM-CSF, producer strain, cultivation, chromatographic purification.

The work was performed in the framework of the State Assignment «Adjustment of the Technology of Preparative Obtaining and Purification of Recombinant Proteins» (no. 13/18).
Conceptos ad hoc, arquitectura cognitiva y localismo léxico
Authors: Campos Millán Laura
Published: July 2019

La Teoría de la Pertinencia hace una de las principales defensas de la tesis de subdeterminación lingüística local. Según esta tesis, el concepto intuitivo expresado por medio de un ítem léxico en la emisión de una oración es un concepto ad hoc que resulta de un proceso libre de control lingüístico. Los conceptos ad hoc se postulan porque  introducen un tipo de organización cognitiva que se considera necesaria para satisfacer las demandas que, para esta Teoría, impone la comprensión de conceptos intuitivos. Aquí planteo que nuestro sistema cognitivo puede satisfacer estas demandas sin tolerar proposiciones determinadas básicamente por conceptos ad hoc. También planteo que la información suministrada por los conceptos ad hoc es dispensable para la comprensión de conceptos intuitivos. Finalmente, propongo una posición léxica local según la cual, el concepto intuitivo expresado a través de un ítem léxico es el concepto léxicamente asociado a ese ítem.
Universalidad en disputa: la lógica de la dominación cultural en el Debate de Valladolid (1550-51)
Authors: Aguerre Lucía Alicia
Published: July 2019

En este artículo se desarrolla una hermeneusis filosófica del “Debate de Valladolid” (1550-51) centrada en la noción de “universalidad”, poniendo de relieve los elementos universalistas presentes tanto en la fundamentación de una praxis de dominación colonial desarrollada por Juan Ginés de Sepúlveda como en el reconocimiento de la diversidad cultural que sostuvo Bartolomé de las Casas. Se estudia, en primer lugar, el contexto histórico-ideológico de la conquista, para luego reparar en los argumentos de de Sepúlveda sobre la barbarie, la superioridad cultural y el derecho de dominio, los cuales establecieron una “lógica de la dominación cultural”. En ese marco, de Sepúlveda pretendió instaurar una fractura entre culturas “civilizadas” y culturas “bárbaras”, en las que la esencia humana se revelaba de manera deficiente. Seguidamente, se analizan las objeciones a la “fractura colonial” presentadas por de las Casas, quien compone una postura alternativa resignificando el concepto de “universalidad” que habitaba los argumentos diferencialistas de de Sepúlveda.
Fernando de Roa y la recepción de la filosofía práctica de Aristóteles
Authors: Contreras Sebastián
Published: July 2019

En este trabajo se expone la manera como Fernando de Roa, catedrático salmantino del siglo xv, recibe la filosofía práctica de Aristóteles. El artículo desarrolla la explicación roense de tres de los tópicos principales de la ética y política clásica: la justicia como virtud social, la servidumbre natural y la felicidad como fin del hombre. Roa, en sus repeticiones, así como en sus comentarios a la Política, se centra en estos problemas y los aborda con un aristotelismo renovado y moderno, que tiene como base las ideas de Pedro de Osma y de los grandes pensadores medievales.
Pragmatismo y evolución: ¿es racional ser teísta y naturalista?
Authors: Rivera-Novoa Ángel
Published: July 2019

Este artículo tiene como objetivo mostrar que 1) la teoría de la evolución es ontológicamente neutral en el debate entre naturalismo metafísico y teísmo y 2) que es consistente sostener en conjunto el naturalismo metodológico y el teísmo. Para ello, examinaré en primer lugar el argumento de Plantinga que intenta mostrar que hay consistencia entre teísmo y evolución y que, por el contrario, hay inconsistencia entre la teoría de la evolución y el naturalismo (tanto metodológico como metafísico). Me interesa aceptar la primera parte del argumento de Plantinga, pero rechazar la segunda. Defiendo que el argumento de Plantinga es válido si y sólo si se supone una teoría de la verdad por correspondencia. A continuación, argumento que si se reemplaza dicha noción por la concepción pragmatista de la verdad de James, es posible concluir 1 y 2.
Definiciones, significado y verdad: aporías de la teoría lingüística de Hobbes
Authors: Saiz Mauro Javier
Published: July 2019

Este trabajo busca recuperar los nodos conflictivos de la teoría de T. Hobbes en torno al lenguaje, la ciencia y la verdad. Recorriendo las lecturas diversas y contradictorias a que estos campos de la obra del filósofo inglés han dado lugar se pretende evidenciar las tensiones inherentes al proyecto científico de Hobbes. No se ofrece una solución final que clausure la discusión, sino que se presentan pautas para lograr identificar las posturas (declaradas e implícitas) del autor que son de difícil conciliación. De este modo, se pretende contribuir a una comprensión más profunda de las múltiples posibilidades que el texto hobbesiano ofrece.
Hannah Arendt y Jean-Luc Marion. El acontecimiento y los márgenes de la metafísica
Authors: García Labrador Julián , Vinolo Stéphane
Published: July 2019

El “acontecimiento” aparece, en la filosofía contemporánea, como el sendero que manifiesta la posibilidad de superar el fin de la metafísica, puesto que rompe tanto con las condiciones metafísicas del aparecer como con las del conocer. Gracias a un análisis comparativo de las obras de Arendt y de Marion, el presente artículo muestra que, aunque el acontecimiento aparezca en tanto que destrucción del concepto moderno de historia en los textos de la primera, y en tanto que modalidad de la saturación de los fenómenos en la fenomenología de la donación de Marion, el uso del acontecimiento nos permite reevaluar la relación que ambos autores mantienen con la metafísica. Después de haber señalado una diferencia fundamental en el uso del acontecimiento que hacen estos dos autores, nos es factible evaluar su posición frente a la posibilidad de seguir pensando después del fin de la metafísica.
Chinese and Malay Interaction in West Kalimantan: Discovering Harmony in Diversity of Ethnic Groups through Local Wisdom
Authors: Atmaja Dwi Surya , Kurnanto M. Edi
Published: July 2019

This research aims to explore the wisdom of socio-religious interaction and tradition in West Kalimantan Malay society towards Chinese ethnic. This research recommends local wisdom as a media to downgrade the tension of social political and religious interactions that have been perceived recently as the threat of national and even global harmony. The source of data in this study was Deutro Malay ethnic and Chinese ethnic at Teluk Pak Kedai. The data was determined through purposive techniques by considering the two circles of key informants, i.e. information from the closest circumference (epicentrum) with the source of wisdom, which in this study consists of a closed circle from the figure of Haji Ismail Mundu and Foundation (China), and information from peripherals. The Data collected through in-depth interviews, observations and documentation were then analyzed by using qualitative normative cycle formats to be presented in thematic classifications in line with the focus and research questions. This study concluded that the theological characteristics of Haji Ismail Mundu can easily adjust to the characteristics of the Chinese. The harmonious reciprocity between the two traditions, in terms of the inter-ethnic reciprocal relationship manifested in the Teluk Pak Kedai, is unique as seen in the respect and appreciation given by the Confucian Chinese in the form of displaying a photo of Haji Ismail Mundu in the Phe Kong worshiping in homes. Every potential of conflict has always been successfully overcome by “intensity” and “modesty” through the figure of Haji Ismail Mundu and being committed to preserve his heritage.
Cultivating Local Wisdom in Character Education: Lessons from Family Education Values of Indonesian Traditional Ceremony
Authors: Makmur Testiani , Dastina Wenny
Published: July 2019

The tradition of establishing a house in Tanjung Pauh, Kerinci contains noble educational values such as deliberation, mutual cooperation, togetherness, knowing and helping each other, monotheism, recalling kindness, and courtesy. Those values are aligned with the concept of character education proposed by Thomas Lickona. The values can be implemented into family education especially in rural communities of Tanjung Pauh, Kerinci. The character values in harvesting rice carried out by the community teach civilized attitude towards plants and sharing with others. Besides, the community also has principles of respect, attention, respect, and compliance with customs regulations. The ceremony is performed based on custom and religious teachings with the intention to reject any disasters experienced by human beings, plants, and animals. Besides, the ceremony performed contains meanings of gratitude to God Almighty in order to reduce the punishment of the grave space, especially for parents who died and owned rice fields. For those who are still alive, these activities are carried out with the hope that everything done runs well, gains healthy bodies and longevity.
Contestation of Religious Identity in the Cyber World: A Case Study of arrahmah.com and VOA Islam Dealing with Religious Others on Facebook
Authors: Mundiri Akmal , Tohet Moch
Published: July 2019

Religious identity frequently causes tensions that in turn lead to social polarization. Contestation and representation of religious identity not only hit the real world but also extend to virtual communities, which are part of a new form of identity contestation in the cyber era. This study uses an ethnographic approach focusing on the beliefs, language, values, rituals, customs, and behavior of people who interact in the community as the object of research. Thus, this study portrays the phenomenon of contestation of religious identity, both in the process of forming the identity (personal identity) of members or followers. The result of this study is that there is the process of forming religious identity on Facebook i.e., in the case of arrahmah.com and VOA Islam. Such process occurs because of the awareness of personal identity, in the form of aspects that differentiate others (self-categorization) and social identity in the form of religious identity that has been internalized through the interaction process in the group. While the demographic contestation of religious identity can be categorized into primary participators as creators of content and religious mindedness narratives, and secondary participators as members who are actively involved in consuming content, and lurkers as silent participants who are targeted by propaganda. The basic form of contestation in these two communities is exclusive religious understanding and views other groups as ‘the other’.
The Association between Abnormal Vaginal Flora and Cytological Evidence of HPV with Prematurity in High-Risk Pregnant Women
Authors: Freire Alana Dantas , Custódio Ada Isa , Filho José Queiroz , Freitas Janaína Crispim , Gonçalves Ana Katherine , Cobucci Ricardo Ney
Published: July 2019

Objective: To evaluate the association between abnormal vaginal flora and cytological evidence of HPV with prematurity in high-risk pregnant women. Study design: A prospective cohort study was designed with high-risk pregnant women who delivered singletons between 20-42 gestational weeks between January to November 2018. Vaginal specimens were collected to obtain material from the upper lateral vaginal vault and cervix for direct microscopic examination of vaginal contents and preparation of Gram-stained and pap smear slides. Potential determinants of infection were assessed using the chi-square test. Poisson regression was used to determine the prevalence ratio and 95% confidence interval of the association between the vaginal flora and cytology with prematurity and a p <0.05 was considered as statistical significance. Results: A total of 68 pap smear and high vaginal swab samples were collected from high-risk pregnant women with a mean age of 30.3 years. There were 26 (38.2%) cases of abnormal vaginal flora and 6 (8.8%) of HPV-induced cytological abnormalities. The preterm delivery rate was comparable between women with normal and abnormal vaginal flora (11.9% vs. 11.5%, prevalence ratio 1.0 [95% confidence interval: 0.8-1.2], aPR 1.0 [95% confidence interval: 0.8-1.2]) and between women with and without abnormal cytology (16.7% vs. 11.3%, prevalence ratio 0.9 [95% confidence interval: 0.6-1.4], aPR 0.9 [95% confidence interval: 0.6-1.4]). Conclusions: We determined no association between abnormal cytology or altered vaginal flora in high-risk pregnancy in terms of overall preterm birth rate.
ХАРАКТЕРИСТИКА НАРУШЕНИЙ РАЗВИТИЯ ПРИ ОБЪЕМНЫХ ЗАБОЛЕВАНИЯХ ГИПОФИЗА У ДЕТЕЙ И ПОДРОСТКОВ
Authors:   Ташкентский педиатрический медицинский институт, Ю.М. Урманова , У.Х. Мавлонов ,   Бухарский областной эндокринологический диспансер
Published: July 2019

The aim of the study was to study the nature of developmental disorders in children and adolescents with masses of the Turkish saddle. Material and methods. 44 children and adolescents with large tumors of the Turkish saddle (TS) were under our supervision in the neuroendocrinology department of the RSNPMCE MH RUz. Of 44 sick girls there were 20, boys 24. The average age of girls was 12.1 years, boys - 12.8 years. By the nature of the pathology, the patients were divided into 5 groups: I group. - inactive pituitary adenomas (NAG) - 23 patients (52.3%); II gr. - Itsenko-Cushing's disease - 5 (11.4%); III gr. - craniopharyngioma - 6 (13.6%); IV gr. - prolactinoma - 5 b-x (11.4%); V gr. - germinoma - 5 bx (11.4%). Results. The results obtained indicate that in children and adolescents there are TJ masses of various origins: inactive pituitary adenomas (52%), craniopharyngiomas (13.6%), corticotropinomas (11.4%), prolactinomas (11.4%), and others. The presence of diabetes insipidus (8.6%) indicates the severity of panhypopituitarism that developed as a result of the tumor. Conclusions: 1) disorders of growth and puberty are the most frequent manifestations of pituitary gland masses (34.0%), 2) pituitary tumors in children and adolescents often lead to hypo- and panhypopituitarism (n = 19, or 43.2%).
Numerical simulation of bedload sediment transport with the ability to model wet/dry interfaces using an augmented Riemann solver
Authors: Barzgaran Mina , Mahdizadeh Hossein , Sharifi Soroosh
Published: July 2019

This paper presents an efficient second-order finite volume method for the simulation of bedload sediment transport which is capable of modeling wet/dry fronts. The governing equations comprise the shallow water equations (SWEs) for the hydrodynamic phase and the Exner equation for the estimation of bedload sediment transport. These sets of equations are then solved using a weakly coupled scheme based on an augmented Riemann solver (WCAR). In this approach, first, the morphodynamic equation is solved, and then, updated bedload changes with the same Riemann structures are used as a source term within the SWEs. The Smart formula is utilized to estimate the bedload sediment discharge within the Exner equation. The proposed numerical model is first used to model a parabolic sediment layer. Then, a bedload hump propagation with an initial subcritical condition is considered. Next, the simulation of dam-break flow over a mobile bed is investigated. Finally, the dam failure due to over-topping is studied and the computed results are compared with available experimental data. Numerical results indicate that the introduced weakly coupled method, developed based upon the augmented Riemann solver, can be effectively used for modeling all investigated flow regimes, including dry-state interfaces.
Evaluation of the effective forecast and decision horizon in optimal hydropower generation considering medium-range precipitation forecasts
Authors: Wei Xu , Xun Yang
Published: July 2019

This paper presents a rolling horizon control (RHC) model to evaluate the effective forecast horizon (EFH) of 10-day forecast inflows derived from quantitative precipitation forecasts (QPFs) and the effective decision horizon (EDH) for hydropower generation. This paper takes the Huanren hydropower reservoir located in the northeast of China as a case study. Firstly, the 10-day forecast inflows are derived from the QPFs. Then the hydropower generation processes are simulated by the RHC model, and the performances of hydropower generation with different EFHs and EDHs are evaluated, respectively. The results show that: (1) the RHC can adapt to varying conditions by re-optimizing the decisions during the EFH; (2) with the EFH increasing, the hydroelectric reliability increases and the efficiency decreases, while the efficiency and reliability are improved with shortened the EDH.
Intercellular Crosstalk Via Extracellular Vesicles in Tumor Milieu as Emerging Therapies for Cancer Progression
Authors: Patras Laura , Banciu Manuela
Published: July 2019

:

Increasing evidence has suggested that extracellular vesicles (EV) mediated bidirectional transfer of functional molecules (such as proteins, different types of RNA, and lipids) between cancer cells and tumor stromal cells (immune cells, endothelial cells, fibroblasts, stem cells) and strongly contributed to the reinforcement of cancer progression. Thus, intercellular EV-mediated signaling in tumor microenvironment (TME) is essential in the modulation of all processes that support and promote tumor development like immune suppression, angiogenesis, invasion and metastasis, and resistance of tumor cells to anticancer treatments.

:

Besides EV potential to revolutionize our understanding of the cancer cell-stromal cells crosstalk in TME, their ability to selectively transfer different cargos to recipient cells has created excitement in the field of tumortargeted delivery of specific molecules for anticancer treatments. Therefore, in tight connection with previous findings, this review brought insight into the dual role of EV in modulation of TME. Thus, on one side EV create a favorable phenotype of tumor stromal cells for tumor progression; however, as a future new class of anticancer drug delivery systems EV could re-educate the TME to overcome main supportive processes for malignancy progression.
Antioxidant Supplementation and Adaptive Response to Training: A Systematic Review
Authors: Pastor Rosario , Tur Josep A.
Published: July 2019

Background:

Antioxidant supplementation has become a common practice among athletes to theoretically
achieve a reduction in oxidative stress, promote recovery and improve performance.

Objective:

To assess the effect of antioxidant supplements on exercise.

Methods:

A systematic literature search was performed up to January 2019 in MEDLINE via EBSCO and Pubmed,
and in Web of Sciences based on the following terms: “antioxidants” [Major] AND “exercise” AND “adaptation”;
“antioxidant supplement” AND “(exercise or physical activity)” AND “(adaptation or adjustment)”
[MesH]. Thirty-six articles were finally included.

Results:

Exhaustive exercise induces an antioxidant response in neutrophils through an increase in antioxidant
enzymes, and antioxidant low-level supplementation does not block this adaptive cellular response. Supplementation
with antioxidants appears to decrease oxidative damage blocking cell-signaling pathways associated with
muscle hypertrophy. However, upregulation of endogenous antioxidant enzymes after resistance training is
blocked by exogenous antioxidant supplementation. Supplementation with antioxidants does not affect the performance
improvement induced by resistance exercise. The effects of antioxidant supplementation on physical
performance and redox status may vary depending on baseline levels.

Conclusion:

The antioxidant response to exercise has two components: At the time of stress and adaptation
through genetic modulation processes in front of persistent pro-oxidant situation. Acute administration of antioxidants
immediately before or during an exercise session can have beneficial effects, such as a delay in the onset of
fatigue and a reduction in the recovery period. Chronic administration of antioxidant supplements may impair
exercise adaptations, and is only beneficial in subjects with low basal levels of antioxidants.
Prophylaxis of Non-communicable Diseases: Why Fruits and Vegetables may be Better Chemopreventive Agents than Dietary Supplements Based on Isolated Phytochemicals?
Authors: Koss-Mikołajczyk Izabela , Baranowska Monika , Todorovic Vanja , Albini Adriana , Sansone Clementina , Andreoletti Pierre , Cherkaoui-Malki Mustapha , Lizard Gérard , Noonan Douglas , Sobajic Sladjana , Bartoszek Agnieszka
Published: July 2019

The World Health Organization (WHO) report from 2014 documented that non-communicable socalled civilization diseases such as cardiovascular disease, chronic respiratory diseases, cancer or type 2 diabetes are responsible for over 50% of all premature deaths in the world. Research carried out over the past 20 years has provided data suggesting that diet is an essential factor influencing the risk of development of these diseases. The increasing knowledge on chemopreventive properties of certain food ingredients, in particular, those of plant origin, opened the discussion on the possibility to use edible plants or their active components in the prevention of these chronic diseases. Health-promoting properties of plant foods are associated with the presence of secondary metabolites that can affect many biological mechanisms of critical importance to the proper functioning of the human organism. Particularly, there have been numerous investigations indicating strong physiological effects of bioactive plant phenols belonging to the flavonoid family. These observations initiated mass production of dietary supplements containing flavonoids commercialized under the name antioxidants, even if their chemical properties did not justify such a term. However, epidemiological studies revealed that isolated bioactive phytochemicals are not as effective as fruits and vegetables containing these substances whereas they are of interest of the functional food industry. In this paper, the critical assessment of reasons for this turn of events has been attempted and the concept of food synergy has been suggested as a future strategy of dietary chemoprevention.
In Silico Molecular Docking, Synthesis of 4-(4-benzoylaminophenoxy) Phenol Derivatives as Androgen Receptor Antagonists
Authors: Elancheran Ramakrishnan , Kabilan Senthamaraikannan , Kotoky Jibon , Ramanathan Muthiah , Bhattacharjee Atanu
Published: July 2019

Aim and Objective:

To study the structural difference, optimization, molecular docking
and development of new benzoyl amino phenoxy phenol derivatives as anti-prostate cancer agents.

Material and Methods:

Strategies towards the identification of novel benzoyl amino phenoxy
phenol (BAPP), molecular docking was performed with the designed Androgen Receptor (AR)
blockers. Pharmacophore-based studies revealed that the nitro- or cyano-substituted anilide groups
have influenced the activity profiles of non-steroidal AR antagonists, followed by the molecular
docking studies with five AR receptors. Molecular docking studies were carried out using Maestro
from Schrödinger. Absorption, Distribution, Metabolism, and Excretion (ADME) properties of the
BAPP derivatives were evaluated for the predictive bioavailability/drug-likeness. These studies
supported vital information for designing new anti-prostate cancer agents.

Results and Discussion:

There are 125 compounds were screened and best fit compounds (12
entries) were well-synthesized in good to excellent yields and anticancer activities were evaluated.
The compounds, 6i showed the highest activities of this series (14.65 ± 1.35 µM).

Conclusion:

The present approach is simple and efficient for the synthesis of BAPP derivatives
and the observed IC50 values of BAPPs were in good agreement with the glide scores obtained
from the molecular docking. We, further, intend to carry out in vitro and in vivo AR binding
studies for the active compounds.
Relating Substructures and Side Effects of Drugs with Chemical-chemical Interactions
Authors: Zhou Bo , Zhao Xian , Lu Jing , Sun Zuntao , Liu Min , Zhou Yilu , Liu Rongzhi , Wang Yihua
Published: July 2019

Background:

Drugs are very important for human life because they can provide treatment, cure, prevention, or diagnosis of different diseases. However, they also cause side effects, which can increase the risks for humans and pharmaceuticals companies. It is essential to identify drug side effects in drug discovery. To date, lots of computational methods have been proposed to predict the side effects of drugs and most of them used the fact that similar drugs always have similar side effects. However, previous studies did not analyze which substructures are highly related to which kind of side effect.

Method:

In this study, we conducted a computational investigation. In this regard, we extracted a drug set for each side effect, which consisted of drugs having the side effect. Also, for each substructure, a set was constructed by picking up drugs owing such substructure. The relationship between one side effect and one substructure was evaluated based on linkages between drugs in their corresponding drug sets, resulting in an Es value. Then, the statistical significance of Es value was measured by a permutation test.

Results and Conclusion:

A number of highly related pairs of side effects and substructures were obtained and some were extensively analyzed to confirm the reliability of the results reported in this study.
Understanding Gene Therapy in Acute Respiratory Distress Syndrome
Authors: Zhang Xue-Peng , Zhang Wei-Tao , Qiu Yue , Ju Min-Jie , Tu Guo-Wei , Luo Zhe
Published: July 2019

Acute Respiratory Distress Syndrome (ARDS) and its complications remain lifethreatening
conditions for critically ill patients. The present therapeutic strategies such as prone positioning
ventilation strategies, nitric oxide inhalation, restrictive intravenous fluid management, and
extracorporeal membrane oxygenation (ECMO) do not contribute much to improving the mortality of
ARDS. The advanced understanding of the pathophysiology of acute respiratory distress syndrome
suggests that gene-based therapy may be an innovative method for this disease. Many scientists have
made beneficial attempts to regulate the immune response genes of ARDS, maintain the normal functions
of alveolar epithelial cells and endothelial cells, and inhibit the fibrosis and proliferation of
ARDS. Limitations to effective pulmonary gene therapy still exist, including the security of viral vectors
and the pulmonary defense mechanisms against inhaled particles. Here, we summarize and review
the mechanism of gene therapy for acute respiratory distress syndrome and its application.
Cystic Fibrosis: New Insights into Therapeutic Approaches
Authors: Tosco Antonella , Villella Valeria R. , Raia Valeria , Kroemer Guido , Maiuri Luigi
Published: July 2019

Since the identification of Cystic Fibrosis (CF) as a disease in 1938 until 2012, only therapies to treat symptoms rather than etiological therapies have been used to treat the disease. Over the last few years, new technologies have been developed, and gene editing strategies are now moving toward a one-time cure. This review will summarize recent advances in etiological therapies that target the basic defect in the CF Transmembrane Receptor (CFTR), the protein that is mutated in CF. We will discuss how newly identified compounds can directly target mutated CFTR to improve its function. Moreover, we will discuss how proteostasis regulators can modify the environment in which the mutant CFTR protein is synthesized and decayed, thus restoring CFTR function. The future of CF therapies lies in combinatory therapies that may be personalized for each CF patient.
Peningkatan keterampilan sosial melalui permainan gobak sodor
Authors: Sudarto Sudarto
Published: July 2019

Penelitian ini bertujuan untuk meningkatkan keterampilan sosial melalui permainan gobak sodor. Penelitian ini merupakan penelitian tindakan kelas yang dilakukan dalam dua siklus. Subjek penelitian ini adalah anak kelompok B TK Panca Setya Kabupaten Sintang yang berjumlah 21 anak. Pengumpulan data dilakukan dengan teknik observasi, wawancara, dan dokumentasi. Analisis data menggunakan teknik deskriptif. Hasil penelitian ini menunjukkan bahwa permainan gobak sodor dapat meningkatkan keterampilan sosial pada anak kelompok B TK Panca Setya Kabupaten Sintang. Hasil ini ditunjukkan dengan hasil peningkatan keterampilan sosial pratindakan bahwa dari 21 anak, 3 anak (14,28%) pada kategori sangat baik. Hasil siklus 1 keterampilan sosial anak meningkat menjadi 10 anak (47,61%) pada kategori baik. Hasil siklus 1 ke siklus 2 mengalami peningkatan keterampilan sosial pada kategori sangat baik menjadi 20 anak (90,47%). Eliciting social skills through gobag sodor game AbstractThe research aimed at eliciting the children’s social skills through gobag sodor game. The study was a classroom action research that consisted of two cycles. The subjects in the study were the students from Group B of Panca Setya Kindergarten the Regency of Sintang and the number of the subjects was 21 children. The data were gathered through observation, interview, and documentation and were analysed using the descriptive technique. The results of the study show that the gobag sodor game is able to elicit the children’s social skill in Group B of Panca Setya Kindergarten in the Regency of Sintang. These results are confirmed by the following findings in terms of social skills. In the pre-action cycle, from 21 subjects 3 subjects 914.28%) belong to very good category. Then, the results of the first cycle show that from 21 subjects 10 subjects (47.61%) belong to good category. Last but not the least, from the first cycle to the second cycle it is found that 20 subjects (90.47%) belong to the very good category.
Model pembelajaran proyek bermuatan budaya lokal dalam meningkatkan kecerdasan interpersonal dan visual-spasial
Authors: Adawiyah Rabihatun
Published: July 2019

Penetian ini bertujuan untuk mengetahui: struktur, kelayakan, dan efektiftas model pembelajaran proyek dengan muatan budaya lokal dalam meningkatkan kecerdasan interpersonal dan visual-spasial anak. Model pengembangan yang digunakan dalam penelitian ini adalah model ADDIE. Model pengembangan ADDIE terdiri dari lima tahap yaitu: Analyze (analisis), design (perancangan), develop (pengembangan), implement (implementasi), dan evaluate (evaluasi). Teknik analisis data menggunakan independent t-test. Hasil dari penelitian ini adalah: (1) struktur pengembangan berupa langkah-langkah model pembelajaran, silabus, RPPM, RPPH, buku panduan guru, (2) produk hasil pengembangan ditinjau dari konsep teori dan media dinyatakan layak digunakan dengan memberikan skor rata-rata “Baik”, (3) terdapat perbedaan yang signifikan antara hasil pre perlakuan dan post perlakuan pada kecerdasan interpersonal dan visual-spasial anak kelompok B TK Aisyiah Bustanul Athfal. Developing project study model local culture content in increasing interpersonal and visual-spatial intelligence AbstractThis research aimed to identify: (1) the structure, the feasibility and the effectiveness of the project learning model with the content of local culture in improving the interpersonal and visual-spatial intelligence of the child. The development model which was used in this research was  ADDIE model. The development model consisted of five steps, that was: analyze, design, develop, implement, and evaluate. Data analysis technique using independent t-test. The conclusion of the development of project learning model with the content of local culture obtained in this research was: (1) development structure was in the form of learning model steps, syllabus, RPPM, RPPH, teacher manual, (2) development result product from the concept of theory and Media is considered feasible to be used by getting the average score of "Good", (3) there is a significant difference between pre treatment and post treatment result on interpersonal and visual-spatial intelligence of children of group B TK Aisyiah Bustanul Athfal, so that the use of project learning model with local culture content is said to be effective in improving the interpersonal and visual-spatial intelligence of the child.
Dampak pemberdayaan masyarakat melalui program biogas dalam mewujudkan kemandirian energi
Authors: Harahap Friska Indria Nora
Published: July 2019

Tujuan penelitian ini adalah untuk menganalisis dampak pemberdayaan masyarakat melalui biogas dalam mewujudkan kemandirian energi. Penelitian ini menggunakan pendekatan kualitatif yang bertujuan untuk menemukan dan menganalisis secara detail dampak pemberdayaan masyarakat dalam program biogas di Kampung Areng Desa Cibodas. Ada beberapa dampak yang ditemukan dalam pemberdayaan melalui program biogas ini, seperti dampak ekonomi, berkaitan dengan pengehamatan yang dirasakan masyarakat setelah menggunakan biogas, dampak ekologi, yang terlihat dari terjaganya lingkungan sekitar masyarakat yang lebih bersih dan rapi, dan yang ketiga adalah dampak sosial. The impact of empowering community through biogas program in manifesting energy independence AbstractThe objective of the study was to analyse the impact of empowering community through biogas in manifesting energy independence. Within the study, qualitative approach was implemented in order to identify and analyse the impacts of empowering community within the biogas program in Kampung Areng, Cibodas Village, in details. Then, there are several impacts that have been found within the community empowerment through the biogas program and these impacts are the economy impact, the ecological impact, and the social impact. The economy impact has been related to the savings that have been resulted from the use of the biogas, while the ecological impact has been related to the maintenance of clean and tidy surrounding environment.
Evaluasi program pendidikan kewirausahaan masyarakat
Authors: Wijayanto Arif , Prasetyo Iis
Published: July 2019

Penelitian ini bertujuan untuk menganalisis: (1) dampak jangka panjang (2) outcome (3) output (4) proses (5) input program PKM di Kabupaten Sleman. Penelitian ini merupakan penelitian evaluasi yang merujuk pada model Logical Framework dengan jenis penelitian kualitatif. Pendekatan evaluasi disusun secara sistematis meliputi evaluasi (a) dampak jangka panjang program, (b) outcome program, (c) output program, (d) proses program, dan (e) input program. Data penelitian dikumpulkan melalui observasi, wawancara dan dokumentasi. Teknik analisis data dilakukan dengan model interaktif. Adapun langkahnya yaitu pengumpulan data, reduksi data, penyajian data, dan verifikasi dan penarikan kesimpulan. Hasil penelitian mengenai evaluasi program PKM di Kabupaten Sleman adalah sebagai berikut (1) Dampak jangka panjang dicapai oleh LKP Silia dan LKP Macell EC; (2) Outcome dicapai oleh LKP Silia, namun tidak dicapai oleh LKP Macell EC; (3) Output dicapai oleh LKP Silia, namun tidak dicapai oleh LKP Macell EC; (4) Proses dicapai oleh LKP Silia, namun tidak dicapai oleh LKP Macel karena penggunaan media di LKP Macell EC kurang maksimal dan kurang mendukung proses pembelajaran; (5) Input yang sesuai dengan prasyarat program telah dicapai oleh LKP Silia, namun tidak berhasil dicapai oleh LKP Macell EC terutama pada prasyarat tutor dan media pembelajaran. Evaluation of community entrepreneurship education program AbstractThis study aims to analyze: (1) long-term impact (2) outcome (3) output (4) process (5) input PKM program in Sleman District. This research is an evaluation research that refers to Logical Framework model with qualitative research type. The evaluation approach is systematically composed including evaluation of (a) the program's long-term impact, (b) program outcomes, (c) program outputs, (d) program processes, and (e) program inputs. The research data was collected through observation, interview and documentation. Data analysis was done by interactive model. The steps are data collection, data reduction, data presentation, and verification and conclusion. The results of research on evaluation of PKM program in Sleman District are as follows (1) long term impact achieved by LKP Silia and LKP Macell EC; (2) Outcome achieved by LKP Silia, but not achieved by LKP Macell EC; (3) Output was achieved by LKP Silia, but not achieved by LKP Macell EC; (4) Process is achieved by LKP Silia, but not reached by LKP Macell EC; (5) Input is achieved or in accordance with the criteria of success by LKP Silia, but on LKP Macell EC.
Evaluasi partisipasi masyarakat pada pembangunan infrastruktur dalam konteks pemberdayaan masyarakat
Authors: Widodo Fatwa
Published: July 2019

Penelitian ini bertujuan untuk mengetahui partisipasi masyarakat pada tahapan perencanaan, implementasi dan hasil dari program pembangunan infrastruktur yang dilakukan oleh Badan Keswadayaan Masyarakat (BKM) Pangudi Mulya. Penelitian ini merupakan penelitian evaluasi dengan pendekatan kualitatif. Model evaluasi yang digunakan ialah CIPP (Context, Input, Prosses dan Product). Teknik pengumpulan data yang digunakan ialah: wawancara, dokumentasi dan observasi sebagai metode penunjang. Teknik analisis data menggunakan reduksi data, display data dan penarikan simpulan. Hasil penelitian menunjukkan bahwa: (1) pada tahap perencanaan warga masyarakat non-anggota ikut berpartisipasi menyuarakan pendapatnya dan ikut serta dalam menyusun rencana program. (2) Pada tahap implementasi, warga non-anggota ikut berpartisipasi dapat dalam bentuk tenaga, materi, donasi maupun logistik. Partisipasi masyarakat dilakukan dengan cara sukarela tanpa adanya paksaan dari anggota BKM. (3) Program pembangunan yang telah dilaksanakan dapat selesai tepat waktu. Hal ini dikarenakan realisasi program dibantu oleh partisipasi masyarakat. Selain itu hasil program dapat merubah keadaan masyarakat, terutama pada sektor kesehatan. An evaluation of social participation in infrastructure development for social empowerment context  AbstractThe aims of this research were to know social participation in the planning; implementation and product stages of the development program in infrastructure by BKM (Badan Keswadayaan Masyarakat) Pangudi Mulya. This research using CIPP (Context, Input, Prosses and Product) model for evaluate the object. The technique of collecting data were: interview, documentation and observation. The technique of analysis data used: reducting data, display data and conclusion. The results of this research were: (1) in the planning step citizen non member did participation such as idea and include to planned a program. (2) In the implementation step, citizen non member also did participation well. Kinds of participation were had variation, such as: power, material, donated and logistic. Citizen non member did participation without compulsion by BKM member, they did voluntarily. (3) The development program were held on timely. It’s because did by participation from citizen. Beside that the result could change social situation, especially on health sector.
Implementasi pembelajaran tematik pada pendidikan anak usia dini
Authors: Kartini Kartini , Waridah Waridah
Published: July 2019

Tujuan penelitian ini adalah untuk mengetahui: (1) Perencanaan pembelajaran tematik  (2) Pelaksanaan pembelajaran tematik, dan (3) Evaluasi pembelajaran tematik di PAUD Kabupaten Melawi. Teknik pegumpulan data  digunakan dengan berbagai setting alamiah yaitu di lembaga dengan pengelola, pendidik, dan peserta didik PAUD di Melawi. Teknik pengumpulan data dalam penelitian meliputi; observasi, wawancara, dan triangulasi. Teknik analisis dalam penelitian melalui keabsahan data dan pemeriksaan, pemeriksaan data dalam penelitian mengikuti kriteria yang sudah ditetapkan yaitu; Kepercayaan (credibility), Keteralihan (transferability), Kebergantungan (depandability), dan Kepastian (confirmability). Hasil penelitian menunjukan bahwa PAUD di Kabupaten Melawi mengimplemntasikan pembelajaran tematik  sudah cukup baik.Hal ini dapat dilihat dalam penyusunan perangkat pembelajaran (Porta, Porsem, RPPM dan RPPH), selain itu penerapan pembelajaran tematik sudah sesusi dengan rinsip-prinsip tematik. Implementation thematic learning early childhood education  AbstractThe purpose of this research is (1) planning thematic learning (2) implementation of thematic learning, and (3) evaluation of thematic learning in Early Age Education Center Melawi. Data collection techniques are used with a variety of natural settings i.e. in institutions with managers, educators, and learners of Early Age Education Center in Melawi. Data collection techniques in the research include; observation, interview, and triangulation. Analysis techniques in the research through the validity of data and examination, the examination of the data in the study follow the criteria already set namely; Trust (credibility), transfer (transferability), Reliance (dependability), and Assurance (conformability). The result of this research is theEarly Age Education Center and implementing thematic Study is already good enough.This can be seen in the preparation of learning devices (Porta, Porsem, RPPM and RPPH), besides that, the application of thematic learning has been consistent with thematic principles.
Застосування автологічної плазми, збагаченої тромбоцитами, при лікуванні остеохондральних дефектів в експерименті на тваринах
Authors: Burianov O.A. , Lyabakh A.P. , Dedukh N.V. , Omelchenko T.M. , Chernovol P.A. , Turchin O.A.
Published: July 2019

Актуальність. Застосування автологічної плазми, збагаченої тромбоцитами (PRP — platelet rich plasma) є перспективною та вельми популярною методикою, що використовується з метою покращання регенерації уражених тканин опорно­рухового апарату. Однак недостатня доказова база обумовлює необхідність проведення досліджень щодо ефективності даного методу лікування. Мета дослідження: в експерименті на 24 тваринах (кролі) шляхом морфологічних досліджень та морфометричного аналізу оцінити ефективність застосування PRP при лікуванні внутрішньосуглобових остеохондральних пошкоджень та дефектів. Матеріали та методи. Морфометричний аналіз препаратів, отриманих від 24 експериментальних тварин, які були розподілені на три групи (2 контрольні та 1 дослідна), проводили для визначення характеру та структури регенерату, його інтеграції до оточуючих тканин, оцінки вмісту клітинних структур та неклітинних елементів, що сформувалися в дефекті в різних серіях експерименту, з використанням модифікованої шкали S.W. O’Driscoll et al. та S.R. Frenkel et al. Результати. Аналіз результатів дослідження з використанням модифікованої шкали гістологічної оцінки показав, що гіаліновий хрящ був наявний тільки в регенераті дослідних тварин серії 3, у яких застосовували PRP. Істотного розшарування поверхні в цих тварин не виявлено, структурна інтегрованість була високою, щільність суглобового хряща навколо країв дефекту значно перевищувала показники контролю. Дегенеративні зміни хондроцитів переважали в контрольних тварин. Перебудова субхондральної кістки під зоною дефекту статистично відрізнялася в дослідній та контрольних групах тварин. При статистичному аналізі виявлено вірогідну різницю між дослідною та контрольними групами (P < 0,001, t = 4,8), що свідчить про ефективність застосування PRP. Висновки. Результати роботи підтвердили ефективність застосування PRP для лікування пацієнтів із внутрішньосуглобовими остеохондральними пошкодженнями та дефектами.
Структурні порушення колінного суглоба при моделюванні остеоартрозу
Authors: Tkachuk P.V. , Strafun S.S. , Savosko S.I. , Makarenko O.M.
Published: July 2019

У статті досліджено структурні зміни капсули колінного суглоба, меніска й суглобової поверхні на моделі остеоартрозу. Встановлено кровонаповнення й стаз судин капсули й ворсинок синовіальної оболонки, загибель клітин ворсинок, дистрофічні зміни хондроцитів меніска й епіфіза кісток суглоба. Дефект суглобової поверхні й перетин хрестоподібної зв’язки позначилися на товщині хряща, редукції клітинного складу й резорбції субхондральної кістки. Зроблено висновок про участь судинного чинника в розвитку дистрофічних і деформаційних змін структурних утворень колінного суглоба при остеоартрозі.