bluuebunny/crossref_metadata_2025 huggingface dataset:
WRN Germline Mutation Is the Likely Inherited Etiology of Various Cancer Types in One Iranian Family
Authors: Norouzi Mahnaz , Shafiei Mohammad , Abdollahi Zeinab , Miar Paniz , Galehdari Hamid , Emami Mohammad Hasan , Zeinalian Mehrdad , Tabatabaiefar Mohammad Amin
Published: June 2021

Background

Familial cancers comprise a considerable distribution of colorectal cancers (CRCs), of which only about 5% occurs through well-established hereditary syndromes. It has been demonstrated that deleterious variants at the newly identified cancer-predisposing genes could describe the etiology of undefined familial cancers.

Methods

The present study aimed to identify the genetic etiology in a 32-year-old man with early onset familial CRC employing several molecular diagnostic techniques. DNA was extracted from tumoral and normal formalin-fixed-paraffin-embedded (FFPE) blocks, and microsatellite instability (MSI) was evaluated. Immunohistochemistry staining of MMR proteins was performed on tumoral FFPE blocks. Next-generation sequencing (NGS), multiplex ligation-dependent amplification (MLPA) assay, and Sanger sequencing were applied on the genomic DNA extracted from peripheral blood. Data analysis was performed using bioinformatics tools. Genetic variants interpretation was based on ACMG.

Results

MSI analysis indicated MSI-H phenotype, and IHC staining proved no expressions of MSH2 and MSH6 proteins. MLPA and NGS data showed no pathogenic variants in MMR genes. Further analysis of NGS data revealed a candidate WRN frameshift variant (p.R389Efs*3), which was validated with Sanger sequencing. The variant was interpreted as pathogenic since it met the criteria based on the ACMG guideline including very strong (PVS1), strong (PS3), and moderate (PM2).

Conclusion

WRN is a DNA helicase participating in DNA repair pathways to sustain genomic stability. WRN deficient function may contribute to CRC development that is valuable for further investigation as a candidate gene in hereditary cancer syndrome diagnosis.
An Immune-Related Signature Predicted Survival in Patients With Kidney Papillary Cell Carcinoma
Authors: Shen Junwen , Wang Rongjiang , Chen Yu , Fang Zhihai , Tang Jianer , Yao Jianxiang , Ling Yuhang , Zhang Lisha , Zhang Xu
Published: June 2021

Immune-related genes are important factors in tumor progression. The main aim of this study was to identify the immune-related genes in kidney papillary cell carcinoma (pRCC) patients. We downloaded RNAseq data and clinical information of pRCC patients from the TCGA database and retrieved the immune-related genes list from Immport. From the data, we mined out 2,468 differential expression genes (DEGs) and 183 immune-related DEGs. Four hub DEGs (NTS, BIRC5, ELN, and CHGA) were identified after conducting Cox analysis and LASSO analysis. Moreover, the prognostic value of the signature based on four hub DEGs was verified using Kaplan–Meier analysis (P = 0.0041 in the training set and p = 0.021 in the test set) and ROC analysis (AUC: 0.957 in 1 year, 0.965 in 2 years, and 0.901 in 3 years in the training set, and 0.963 in 1 year, 0.898 in 2 years, and 0.742 in 3 years in the test set). Furthermore, we found that the high-risk score group had a higher percentage of B cells in the immune component, a higher expression of immune-related genes (CTLA4, LAG3, PDCD1LG2, and TIGIT), and a better immunotherapy response.
A Retrospective Review Following the Addition of Clonidine to a Neonatal Abstinence Syndrome Treatment Algorithm
Authors: Bader Mohammad Y. , Zaghloul Nahla , Repholz Ashley , Nagy Nadia , Ahmed Mohamed N. , Thompson Leslie , Kylat Ranjit I.
Published: June 2021

Objective: To investigate the outcomes associated with the implementation of a neonatal abstinence syndrome (NAS) treatment algorithm utilizing dual therapy with morphine sulfate and clonidine in a level four neonatal intensive care unit (NICU).

Study Design: A cohort of neonates (≥35 weeks gestation) born at an academic tertiary medical center between January 1, 2015 and December 31, 2018 who were diagnosed with NAS were retrospectively evaluated following the implementation of a new NAS treatment algorithm. Neonates were categorized in two groups based on if they were treated pre- or post-implementation of the protocol. The primary efficacy outcome was length of hospital stay. Secondary outcomes included the incidence of adverse drug reactions, length of treatment for NAS, and maximum as well as total cumulative dose of each medication used to treat NAS.

Results: The implementation of this NAS treatment algorithm significantly reduced the length of hospital stay (30 days vs. 20 days, p = 0.001). In addition, there was a significant decrease in duration of morphine sulfate exposure as well as cumulative dose of morphine required to successfully treat a neonate with NAS in the post-implementation group (26 days vs. 15 days, p = 0.002 and 6.9 mg/kg vs. 3.4 mg/kg, p = 0.031).

Conclusion: Addition of clonidine to morphine sulfate as initial therapy for NAS significantly reduced the cumulative exposure as well as duration of exposure to morphine sulfate compared to morphine monotherapy and decrease length of hospital stay.
Naringenin Attenuates Non-Alcoholic Fatty Liver Disease by Enhancing Energy Expenditure and Regulating Autophagy via AMPK
Authors: Yang Ying , Wu Yue , Zou Jie , Wang Yu-Hao , Xu Meng-Xia , Huang Wei , Yu Dao-Jiang , Zhang Li , Zhang Yuan-Yuan , Sun Xiao-Dong
Published: June 2021

Background: The prevalence of non-alcoholic fatty liver disease (NAFLD) keeps growing recently.

Purpose: To investigate the effects and mechanisms of naringenin (NAR) on NAFLD.

Methods: High-fat diet (HFD)-induced NAFLD rats were orally administered with NAR at 10, 30, and 90 mg/kg for 2 weeks. The serum level of triglyceride (TG), total cholesterol (TC), glutamic-oxaloacetic transaminase (AST), and glutamic-pyruvic transaminase (ALT) was measured. The hepatic histology was detected by H&E and oil red O staining. L02 and Huh-7 cells were induced by sodium oleate to establish a NAFLD cell model. The effects of NAR on lipid accumulation were detected by oil red O staining. The glucose uptake and ATP content of 3T3-L1 adipocytes and C2C12 myotubes were measured. The expression of proteins of the AMPK signaling pathway in 3T3-L1 adipocytes and C2C12 myotubes was assessed by Western blotting. The mitochondrial biogenesis of 3T3-L1 adipocytes and C2C12 myotubes was measured by mitotracker orange staining and Western blotting. The biomarkers of autophagy were detected by Western blotting and immunofluorescence. The binding of NAR to AMPKγ1 was analyzed by molecular docking. Chloroquine and compound C were employed to block autophagic flux and AMPK, respectively.

Results: NAR alleviated HFD-induced NAFLD in rats at 10, 30, and 90 mg/kg. NAR attenuated lipid accumulation in L02 and Huh-7 cells at 0.7, 2.2, 6.7, and 20 μM. NAR increased glucose uptake, decreased the ATP content, activated the CaMKKβ/AMPK/ACC pathway, and enhanced the mitochondrial biogenesis in 3T3-L1 adipocytes and C2C12 myotubes. NAR increased autophagy and promoted the initiation of autophagic flux in 3T3-L1 preadipocytes and C2C12 myoblasts, while it inhibited autophagy in NAFLD rats, 3T3-L1 adipocytes, and C2C12 myotubes. Molecular docking showed that NAR binds to AMPKγ1. Compound C blocked effects of NAR on lipid accumulation and autophagy in L02 cells.

Conclusion: NAR alleviates NAFLD by increasing energy expenditure and regulating autophagy via activating AMPK directly and indirectly. The direct binding of NAR and AMPKγ1 needs further validation.
Control of the Rhizobia Nitrogen-Fixing Symbiosis by Common Bean MADS-Domain/AGL Transcription Factors
Authors: Ayra Litzy , Reyero-Saavedra María del Rocio , Isidra-Arellano Mariel C. , Lozano Luis , Ramírez Mario , Leija Alfonso , Fuentes Sara-Isabel , Girard Lourdes , Valdés-López Oswaldo , Hernández Georgina
Published: June 2021

Plants MADS-domain/AGL proteins constitute a large transcription factor (TF) family that controls the development of almost every plant organ. We performed a phylogeny of (ca. 500) MADS-domain proteins from Arabidopsis and four legume species. We identified clades with Arabidopsis MADS-domain proteins known to participate in root development that grouped legume MADS-proteins with similar high expression in roots and nodules. In this work, we analyzed the role of AGL transcription factors in the common bean (Phaseolus vulgaris) – Rhizobium etli N-fixing symbiosis. Sixteen P. vulgaris AGL genes (PvAGL), out of 93 family members, are expressed – at different levels – in roots and nodules. From there, we selected the PvAGL gene denominated PvFUL-like for overexpression or silencing in composite plants, with transgenic roots and nodules, that were used for phenotypic analysis upon inoculation with Rhizobium etli. Because of sequence identity in the DNA sequence used for RNAi-FUL-like construct, roots, and nodules expressing this construct -referred to as RNAi_AGL- showed lower expression of other five PvAGL genes highly expressed in roots/nodules. Contrasting with PvFUL-like overexpressing plants, rhizobia-inoculated plants expressing the RNAi_AGL silencing construct presented affection in the generation and growth of transgenic roots from composite plants, both under non-inoculated or rhizobia-inoculated condition. Furthermore, the rhizobia-inoculated plants showed decreased rhizobial infection concomitant with the lower expression level of early symbiotic genes and increased number of small, ineffective nodules that indicate an alteration in the autoregulation of the nodulation symbiotic process. We propose that the positive effects of PvAGL TF in the rhizobia symbiotic processes result from its potential interplay with NIN, the master symbiotic TF regulator, that showed a CArG-box consensus DNA sequence recognized for DNA binding of AGL TF and presented an increased or decreased expression level in roots from non-inoculated plants transformed with OE_FUL or RNAi_AGL construct, respectively. Our work contributes to defining novel transcriptional regulators for the common bean – rhizobia N-fixing symbiosis, a relevant process for sustainable agriculture.
Mentalizing Bodies: Explicit Mentalizing Without Words in Psychotherapy
Authors: Jensen Thomas Wiben , Høgenhaug Stine Steen , Kjølbye Morten , Bloch Marie Skaalum
Published: June 2021

Introduction: Mentalization concerns the human ability to understand the actions of others (and oneself) in terms of intentional mental states. Theoretically, the notion has been described via the poles of automatic, non-verbal implicit mentalization as opposed to conscious and verbal explicit mentalization. In this article, we challenge this standard distinction by examining examples from psychotherapy. We argue that explicit mentalization can also be carried out via embodied non-verbal actions.

Method: Four cases of real-life interaction from psychotherapy sessions are analyzed from the qualitative perspective of embodied cognition and multimodal interaction analysis. The analyses are based on video data transformed into transcriptions and anonymized drawings from a larger cognitive ethnography study conducted at a psychiatric hospital in Denmark.

Results: The analyses demonstrate the gradual development from predominantly implicit mentalizing to predominantly explicit mentalizing. In the latter part of the examples, the mentalizing activity is initiated by the therapist on an embodied level but in an enlarged and complex manner indicating a higher level of awareness, imagination, and reflection. Thus, the standard assumption of explicit mentalization as contingent on verbal language is challenged, since it is demonstrated how processes of explicit mentalization can take place on an embodied level without the use of words.

Conclusion: Based on real-life data, the study demonstrates that online processes of implicit and explicit mentalization are gradual and interwoven with embodied dynamics in real-life interaction. Thus, the analyses establish a window into how mentalization is carried out by psychotherapists through interaction, which testifies to the importance of embodied non-verbal behavior in psychotherapy. Further, informed by the notion of affordance-space, the study points to alternative ways of conceptualizing the intertwined nature of bodies and environment in relation to conveying more complex understandings of other people.
Effects of Wise Intervention on Perceived Discrimination Among College Students Returning Home From Wuhan During the COVID-19 Outbreak
Authors: Lu Ting , Guo Zihan , Li Hao , Zhang Xinyu , Ren Zhihong , Yang Weiping , Wei Liuqing , Huang Ling
Published: June 2021

At the beginning of the coronavirus disease 2019 (COVID-19) outbreak, college students returning home from Wuhan, Hubei Province, experienced various degrees of discrimination. This study first investigates perceived discrimination among college students returning home from Wuhan. Then, an experimental method is used to investigate the effectiveness of an intervention designed to reduce the perceived discrimination among those who returned to towns outside of Hubei Province. A total of 63 college students participated in the experiment. In the experimental group (N = 31), a wise intervention based on reading and writing was adopted to intervene in perceived discrimination among the participants. The results showed that the perceived discrimination among students returning from Wuhan to towns outside of Hubei Province was significantly higher than that among students returning to towns within Hubei Province. The wise intervention reduced the perceived discrimination in the experimental group but not in the control group. Further analysis found that perceived social support fully mediated the relationship between the intervention and perceived discrimination. These results provide insights on how the content of intervention (perceived social support) and the form of intervention (wise intervention) can prevent the occurrence of psychological problems in epidemic situations.
The Impact of Unplanned School Closures on Adolescent Behavioral Health During the Covid-19 Pandemic in Malaysia
Authors: Amran Muhammad Syawal , Jamaludin Khairul Azhar
Published: June 2021

School closures were implemented as a public health intervention to reduce the risk of infection from COVID-19. However, prolonged school closure is likely to impact adolescents' behavioral health due to the extreme change in routine. The current study aimed to explore adolescents' behavioral health experiences during the beginning of the outbreak of the COVID-19 pandemic. This study was done using qualitative methods to interview 15 adolescent participants (n =15) from low-income households in Malaysia. The study lasted for 2 months amidst the outbreak and data were collected via online based on focus group discussions. The results revealed that adolescents faced four main themes of experience during the COVID-19 pandemic: Alteration of sleep patterns, stress-related fatigue, dysfunctional eating patterns and lack of physical activity. This first-hand experience shows that knowledge and skills of adolescents' behavioral practices during outbreak deserves attention. This research stresses the role of family, schools, and media in addressing the health communication gap among adolescents to help them adapt in these new norms.
Counseling Techniques Supporting West African Children With Adverse Childhood Experiences: A Systematic Review
Authors: Clay Shanelle V. , Jackson Cheria , Stephenson Quincy
Published: June 2021

Using a meta-synthesis approach, through the review of current literature, five published and peer reviewed qualitative research reports were studied. The intention was to identify interventions being used with West African children who have endured adverse childhood experiences. These results were found through matching inclusionary criteria and all studies were screened for appropriateness and relevance to the topic matter. The literature was analyzed across five online databases including Proquest, PsychInfo, Scopus, Wiley, and Springer from January 2005 to June 2020. The authors found minimal evidence indicating interventions used in West Africa for adverse experiences related specifically to children, but found themes related to interventions that serve West African families that include children. Findings were thematically analyzed through meta-synthesis and identified four themes used in the interventions, which include western, spiritual, expressive arts, and cultural approaches. West African children endure adverse experiences such as terrorism, abuse, and war violence that contribute to an increasing the need for mental health interventions. These experiences approached from western, spiritual, expressive arts, and cultural vantage points were identified but limited in information about delivery and efficacy, thus providing little guidance regarding further exploratory research.
Effect of Vegetable Oils Feed Additives on Endoparasites Associated with Dewormed Racing Horses
Authors: Górniak Wanda , Moniuszko Hanna , Wojnarowski Konrad , Górniak Aleksander , Cholewińska Paulina , Waliczek Agnieszka , Soroko Maria , Szeligowska Natalia
Published: June 2021

The effectiveness of commonly used parasiticides decreases due to the drug resistance developed by many organisms. Therefore, the application of feed additives possessing antiparasitic properties may be helpful in limiting the burden of parasites. The aim of this research was to evaluate parasitological coefficients for endoparasites of regularly dewormed Arabian horses and Thoroughbreds, fed on a basal diet of oat and muesli with vegetable oils and/or without vitamin E. The observations revealed parasitic invasion in 25 of 27 examined horses. The most frequent in both breeds were Strongylidae—accounting for 86% of Thoroughbreds and 100% of Arabian horses. Strongyloididae were observed in 33% of individuals representing both breeds while botflies were found in one Arabian horse and one Thoroughbred. Mean EPG coefficients for strongylids and strongyloidids were higher in Thoroughbreds (530 vs. 529 and 43 vs. 29, respectively), although the differences were not statistically significant. The addition of pure linseed oil significantly reduced the number of Strongylidae in fecal samples. The results indicate that properly selected plant additives obtained from arable crops may be helpful in limiting the numbers of drug-resistant strongylids.
Novel Poly-(Lactic-Co-Glycolic Acid) Targeted Nanoparticles Conjunct with Antibody for the Enhancement of Antibacterial Activity against Ralstonia solanacearum
Authors: Yang Xue-Jun , Geng Li-Tian , Xu Xiao-Yi , Shen Xiang-Yu , Sheng Sheng , Wu Fu-An , Wang Jun
Published: June 2021

Due to the strong pathogenicity of Ralstonia solanacearum, a variety of strategies have been used to develop antibacterial agents; however, antibacterial drugs with targeted effects on R. solanacearum remain lacking. Herein, we present a nanoagent targeting R. solanacearum based on our previous research on poly-(lactic-co-glycolic acid) (PLGA) particles (PLGA-NPs) loaded with methyl caffeate and caffeic acid phenethyl ester. Antibodies that have specific effects on R. solanacearum, which were verified using immuno-PCR, were first used to prepare PLGA-targeted nanoparticles (PLGA-TNPs). The antibody coupling process was investigated in terms of antibody binding degree and antibacterial activity. The EC50 value of PLGA-TNPs was 0.021 mg/mL, which was significantly reduced by 92% in comparison to PLGA-NPs. PLGA-TNPs had a perforating effect on the cell membrane of R. solanacearum, but no effects on Escherichia coli according to the SEM results. In addition, a downregulation of the pathogenicity-related genes compared to PLGA-NP treatment was observed, and the expression of egl, phcA, phcB, pilT, polA-238, and pehC decreased by 78, 79, 87, 61, 58, and 41%, respectively. Therefore, PLGA-targeted nanoparticles not only enhance the activity against R. solanacearum, but also provide a new idea for controlling bacterial wilt.
Strip Tillage and Crop Residue Retention Decrease the Size but Increase the Diversity of the Weed Seed Bank under Intensive Rice-Based Crop Rotations in Bangladesh
Authors: Hossain Mohammad Mobarak , Begum Mahfuza , Hashem Abul , Rahman Md. Moshiur , Ahmed Sharif , Hassan Montaser M. , Javed Talha , Shabbir Rubab , Hadifa Adel , Sabagh Ayman EL , Bell Richard W.
Published: June 2021

Cropping under conservation agriculture (CA) has become increasingly attractive among farmers in recent years. However, weed control may be more difficult during the transition to CA from conventional establishment methods due to the reduction in tillage intensity. Conversely, CA changes to weed dynamics can alter the weed seed bank in the longer run. In Bangladesh’s intensively cropped rice-based rotations, the nature of weed seed bank shifts over time after adopting CA are poorly known. Two 2-year studies were sampled from on-farm CA experiments under wheat-mungbean-winter rice and monsoon rice-mustard-winter rice rotations. We investigated the effects of reduced soil disruption in the form of strip-tillage (ST) combined with increased deposition of standing reside from previous crops (0 vs. 50%). The weed seed bank in 0–5, 5–10, and 10–15 cm depths of soil were quantified in a shade-house experiment by measuring weed emergence over 12 months in seedling trays. After 2 years of field study, the year-round count of emerged weeds from the seed bank showed that ST plus 50% mulch had a lower weed abundance and biomass and fewer weed species than that of conventional tillage (CT) without residue. The perennial weeds Ageratum conyzoides L., Alternanthera philoxeroides L., Cynodon dactylon L., Cyperus rotundus L., Jussia decurrence Walt., Leersia hexandra L., Scirpus mucronatus (L.) Palla., and Solanum torvum Sw. were enriched in the smaller-sized ST seed banks in terms of both density and biomass. The CT, on the other hand, was dominated by annual weeds: Cyperus difformis L., Cyanotis axillaris Roem., Echinochloa crus-galli (L.) Beauv., Eleusine indica L., Fimbristylis miliacea (L.) Vahl., and Rotala ramosior L. Overall, ST plus 50% residue had a more diverse seed bank than CT without mulch. The majority of weed seeds were amassed in the 0–5 cm soil depth of the ST, while most of them were accumulated in the 10–15 cm layer of the CT. The wheat-mungbean-winter rice rotation had a more diverse floristic composition with many more weed species than the monsoon rice-mustard-winter rice rotation.
Optimized Supercritical CO2 Extraction Enhances the Recovery of Valuable Lipophilic Antioxidants and Other Constituents from Dual-Purpose Hop (Humulus lupulus L.) Variety Ella
Authors: Nagybákay Nóra Emilia , Syrpas Michail , Vilimaitė Vaiva , Tamkutė Laura , Pukalskas Audrius , Venskutonis Petras Rimantas , Kitrytė Vaida
Published: June 2021

The article presents the optimization of supercritical CO2 extraction (SFE-CO2) parameters using response surface methodology (RSM) with central composite design (CCD) in order to produce single variety hop (cv. Ella) extracts with high yield and strong in vitro antioxidant properties. Optimized SFE-CO2 (37 MPa, 43 °C, 80 min) yielded 26.3 g/100 g pellets of lipophilic fraction. This extract was rich in biologically active α- and β-bitter acids (522.8 and 345.0 mg/g extract, respectively), and exerted 1481 mg TE/g extract in vitro oxygen radical absorbance capacity (ORAC). Up to ~3-fold higher extraction yield, antioxidant recovery (389.8 mg TE/g pellets) and exhaustive bitter acid extraction (228.4 mg/g pellets) were achieved under the significantly shorter time compared to the commercially used one-stage SFE-CO2 at 10–15 MPa and 40 °C. Total carotenoid and chlorophyll content was negligible, amounting to <0.04% of the total extract mass. Fruity, herbal, spicy and woody odor of extracts could be attributed to the major identified volatiles, namely β-pinene, β-myrcene, β-humulene, α-humulene, α-selinene and methyl-4-decenoate. Rich in valuable bioactive constituents and flavor compounds, cv. Ella hop SFE-CO2 extracts could find multipurpose applications in food, pharmaceutical, nutraceutical and cosmetics industries.
Effects of the Quinone Oxidoreductase WrbA on Escherichia coli Biofilm Formation and Oxidative Stress
Authors: Rossi Federico , Cattò Cristina , Mugnai Gianmarco , Villa Federica , Forlani Fabio
Published: June 2021

The effects of natural compounds on biofilm formation have been extensively studied, with the goal of identifying biofilm formation antagonists at sub-lethal concentrations. Salicylic and cinnamic acids are some examples of these compounds that interact with the quinone oxidoreductase WrbA, a potential biofilm modulator and an antibiofilm compound biomarker. However, WrbA’s role in biofilm development is still poorly understood. To investigate the key roles of WrbA in biofilm maturation and oxidative stress, Escherichia coli wild-type and ∆wrbA mutant strains were used. Furthermore, we reported the functional validation of WrbA as a molecular target of salicylic and cinnamic acids. The lack of WrbA did not impair planktonic growth, but rather affected the biofilm formation through a mechanism that depends on reactive oxygen species (ROS). The loss of WrbA function resulted in an ROS-sensitive phenotype that showed reductions in biofilm-dwelling cells, biofilm thickness, matrix polysaccharide content, and H2O2 tolerance. Endogenous oxidative events in the mutant strain generated a stressful condition to which the bacterium responded by increasing the catalase activity to compensate for the lack of WrbA. Cinnamic and salicylic acids inhibited the quinone oxidoreductase activity of purified recombinant WrbA. The effects of these antibiofilm molecules on WrbA function was proven for the first time.
Hydroquinone Exposure Worsens Rheumatoid Arthritis through the Activation of the Aryl Hydrocarbon Receptor and Interleukin-17 Pathways
Authors: Heluany Cintia Scucuglia , Donate Paula Barbim , Schneider Ayda Henriques , Fabris André Luis , Gomes Renan Augusto , Villas-Boas Isadora Maria , Tambourgi Denise Vilarinho , Silva Tarcilia Aparecida da , Trossini Gustavo Henrique Goulart , Nalesso Giovanna , Silveira Eduardo Lani Volpe , Cunha Fernando Queiroz , Farsky Sandra Helena Poliselli
Published: June 2021

Rheumatoid arthritis (RA) development is strongly associated with cigarette smoke exposure, which activates the aryl hydrocarbon receptor (AhR) as a trigger for Th17 inflammatory pathways. We previously demonstrated that the exposure to hydroquinone (HQ), one of the major compounds of cigarette tar, aggravates the arthritis symptomatology in rats. However, the mechanisms related to the HQ-related RA still remain elusive. Cell viability, cytokine secretion, and gene expression were measured in RA human fibroblast-like synoviocytes (RAHFLS) treated with HQ and stimulated or not with TNF-α. Antigen-induced arthritis (AIA) was also elicited in wild type (WT), AhR −/− or IL-17R −/− C57BL/6 mice upon daily exposure to nebulized HQ (25ppm) between days 15 to 21. At day 21, mice were challenged with mBSA and inflammatory parameters were assessed. The in vitro HQ treatment up-regulated TNFR1, TNFR2 expression, and increased ROS production. The co-treatment of HQ and TNF-α enhanced the IL-6 and IL-8 secretion. However, the pre-incubation of RAHFLS with an AhR antagonist inhibited the HQ-mediated cell proliferation and gene expression profile. About the in vivo approach, the HQ exposure worsened the AIA symptoms (edema, pain, cytokines secretion and NETs formation) in WT mice. These AIA effects were abolished in HQ-exposed AhR −/− and IL-17R −/− animals though. Our data demonstrated the harmful HQ influence over the onset of arthritis through the activation and proliferation of synoviocytes. The HQ-related RA severity was also associated with the activation of AhR and IL-17 pathways, highlighting how cigarette smoke compounds can contribute to the RA progression.
Highlighting Specific Features to Reduce Chemical and Thermal Risks of Electronic Cigarette Use through a Technical Classification of Devices
Authors: Soulet Sébastien , Duquesne Marie , Pairaud Charly , Toutain Jean
Published: June 2021

Currently, the emission generation protocol of electronic cigarettes has only one standardized vaping regimen that is consistent with mouth-to-lungs inhalation. Recent works show the significant increase in performance of the device with the use of a direct lung vaping regimen (167 mL s−1, consistent with direct lung inhalation). However, requirements are needed for its use in a laboratory. This work aims at identifying mechanical characteristics of a device and providing a classification based on recommended power range, electrical resistance, heating surface, and air resistance of twenty-six tested devices. The electrical resistivity relation allows the estimation of the wire surface using its diameter and its length. The air resistance is obtained by measuring the pressure drop of the tested device with airflow rates ranging from 1–10 L min−1. Through the wide panel of tested devices, results allow separating them in two categories: classical and sub-ohm electronic cigarettes consistent with the two inhalation behaviours. Differences up to 71 mm2 for the wire surface and up to 4.8 Pa 0.5 min L−1 for the air resistance are observed between them. This limit seems to correspond to a required power of 25 W and an electrical resistance of 1.1 Ω.
Texture Identification of Objects Using a Robot Fingertip Module with Multimodal Tactile Sensing Capability
Authors: Bok Bo-Gyu , Jang Jin-Seok , Kim Min-Seok
Published: June 2021

Modern robots fall behind humans in terms of the ability to discriminate between textures of objects. This is due to the fact that robots lack the ability to detect the various tactile modalities that are required to discriminate between textures of objects. Hence, our research team developed a robot fingertip module that can discriminate textures of objects via direct contact. This robot fingertip module is based on a tactile sensor with multimodal (3-axis force and temperature) sensing capabilities. The multimodal tactile sensor was able to detect forces in the vertical (Z-axis) direction as small as 0.5 gf and showed low hysteresis error and repeatability error of less than 3% and 2% in the vertical force measurement range of 0–100 gf, respectively. Furthermore, the sensor was able to detect forces in the horizontal (X- and Y-axes) direction as small as 20 mN and could detect 3-axis forces with an average cross-talk error of less than 3%. In addition, the sensor demonstrated its multimodal sensing capability by exhibiting a near-linear output over a temperature range of 23–35 °C. The module was mounted on a motorized stage and was able to discriminate 16 texture samples based on four tactile modalities (hardness, friction coefficient, roughness, and thermal conductivity).
Multimodal Summarization of User-Generated Videos
Authors: Psallidas Theodoros , Koromilas Panagiotis , Giannakopoulos Theodoros , Spyrou Evaggelos
Published: June 2021

The exponential growth of user-generated content has increased the need for efficient video summarization schemes. However, most approaches underestimate the power of aural features, while they are designed to work mainly on commercial/professional videos. In this work, we present an approach that uses both aural and visual features in order to create video summaries from user-generated videos. Our approach produces dynamic video summaries, that is, comprising the most “important” parts of the original video, which are arranged so as to preserve their temporal order. We use supervised knowledge from both the aforementioned modalities and train a binary classifier, which learns to recognize the important parts of videos. Moreover, we present a novel user-generated dataset which contains videos from several categories. Every 1 s part of each video from our dataset has been annotated by more than three annotators as being important or not. We evaluate our approach using several classification strategies based on audio, video and fused features. Our experimental results illustrate the potential of our approach.
Experimental Study of See-Saw Mode Nano-Vibration on Orifice-Type Restrictors
Authors: Shen Xiaoyan , Yu Jing , Yin Jianlong , Li Dongsheng
Published: June 2021

Slide stability is key to the aerostatic guide in ultra-precise machines; thus, it has garnered plenty of attention. Macro-scale studies are commonplace, but micro- and nano-vibration issues require more attention. Microscope vibration is mainly caused by tiny changes in the fluid parameters of lubricating gas film, which is complex and has no verdict. In this case, slide-gas interaction should be considered. In this study, the widely used orifice-type restrictor was investigated for its nano-vibration performance. A Comsol finite-element-method fluid–structure interaction model was used to simulate and analyze an orifice-type restrictor, and orifice-restrictor vibration characteristics at the nanometer scale were inspected using a high-performance laser vibrometer. The results demonstrate that see-saw mode vibrations occur in the restrictors, growing stronger with increased air-supply pressure. The see-saw vibration’s axis is speculatively determined based on orifice and restrictor structures, and the vibration type is related to the number of orifices. The results also show that the vibration is random with natural frequencies at the kilohertz level. The newly provided research results are beneficial for better understanding the nano-vibrations of orifice-type restrictors.
Impact of Herbicide Treatments on the Construction Materials in the Roman Wall of Lugo, Spain (UNESCO World Heritage Site)
Authors: Prieto Beatriz , Sanmartín Patricia , Cancelo-González Javier , Torres Lucía , Silva Benita
Published: June 2021

Combined laboratory and field research examining the possible alterations caused by herbicide treatments applied to the construction materials (schist and some granite, bound with mortar) in the Roman wall of Lugo (NW Spain), declared a World Heritage site by UNESCO in 2000, was performed in three separate studies in the past 20 years. In the summers of 1998 and 1999, the herbicides glyphosate, sulphosate and glufosinate–ammonium, as well as physical treatments (infrared and burning) were separately applied to different areas of the wall. In the spring of 2016, the oxyfluorfen herbicide Goal Supreme® was applied to test areas. In the winter of 2018, three essential oils, Origanum vulgare L., Thymus zygis Loefl. ex L., and Thymus vulgaris L., were each applied to test areas. Mineralogical modifications in the materials (determined by X-ray diffraction analysis), as well as visible physical changes, such as colour changes, and the appearance of saline residues were evaluated after the treatments. In the 1998/9 trial, glyphosate and both physical treatments triggered changes in the vermiculite clay minerals in the schists, and the physical treatments also caused changes in the kaolinite. None of the treatments caused highly perceptible colour changes. The oxyfluorfen herbicide did not cause any mineralogical alterations in the construction materials, but it did generate an increase in chloride, nitrate and sulphate contents of the granite and a slight darkening of this material. In the most recent study, the only deleterious effect observed was a perceptible increase in lightness and reduction in the yellow component after the application of Thymus zygis Loefl. ex L. essential oil to granite.
A Preliminary Study on the Use of Xylit as Filter Material for Domestic Wastewater Treatment
Authors: Spychała Marcin , Nawrot Tadeusz , Matz Radosław
Published: June 2021

The aim of the study was to verify two morphological forms (“angel hair” and “scraps”) of xylit as a trickling filter material. The study was carried out on two types of polluted media: septic tank effluent (STE) and seminatural greywater (GW). The basic wastewater quality indicators, namely, chemical oxygen demand (COD), biochemical oxygen demand (BOD5), total suspended solids (TSS), ammonium nitrogen (NNH4), and total phosphorus (Ptot) were used as the indicators of treatment efficiency. Filtering columns filled with the investigated material acted as conventional trickling filters at a hydraulic load of 376–472 cm3/d during the preliminary stage, 198–245 cm3/d during stage I, and 184–223 cm3/d during stage II. The removal efficiency of the two morphological forms of xylit did not differ significantly. The average efficiencies of treatment were as follows: for COD, over 70, 80, and 85% for preliminary stage, stage I and stage II, respectively; for BOD5, 77–79% (preliminary stage); for TSS, 42% and 70% during the preliminary stage, and 88, 91, and 65% during stage I; for NNH4, 97–99% for stage I and 36–49% for stage II; for Ptot, 51–54% for stage I and 52–56% for stage II. The study demonstrated that xylit was a material highly effective in wastewater quality indicators removal, even during the initial period of its use.
Calibration of Finn Model and UBCSAND Model for Simplified Liquefaction Analysis Procedures
Authors: Chou Jui-Ching , Yang Hsueh-Tusng , Lin Der-Guey
Published: June 2021

Soil-liquefaction-related hazards can damage structures or lead to an extensive loss of life and property. Therefore, the stability and safety of structures against soil liquefaction are essential for evaluation in earthquake design. In practice, the simplified liquefaction analysis procedure associated with numerical simulation analysis is the most used approach for evaluating the behavior of structures or the effectiveness of mitigation plans. First, the occurrence of soil liquefaction is evaluated using the simplified procedure. If soil liquefaction occurs, the resulting structural damage or the following mitigation plan is evaluated using the numerical simulation analysis. Rational and comparable evaluation results between the simplified liquefaction analysis procedure and the numerical simulation analysis are achieved by ensuring that the liquefaction constitutive model used in the numerical simulation has a consistent liquefaction resistance with the simplified liquefaction analysis procedure. In this study, two frequently used liquefaction constitutive models (Finn model and UBCSAND model) were calibrated by fitting the liquefaction triggering curves of most used simplified liquefaction analysis procedures (NCEER, HBF, JRA96, and T-Y procedures) in Taiwan via FLAC program. In addition, the responses of two calibrated models were compared and discussed to provide guidelines for selecting an appropriate liquefaction constitutive model in future projects.
3D In Situ Stress Estimation by Inverse Analysis of Tectonic Strains
Authors: Guo Mingwei , Yin Shunde , Li Chunguang , Wang Shuilin
Published: June 2021

The determination of a 3D engineering-scale in situ stress field is essential in underground rock mechanics and engineering. The inverse analysis method is a useful technique to determine the in situ stress around the zone of interest. This paper presents a new approach with tectonic strains based on traditional stress-based or displacement-based inverse analysis. In this approach, there are only six tectonic strain variables at the boundary to be optimized, which does not need to select the stress or displacement boundary conditions as the traditional inverse analysis. Therefore, the proposed approach has a better clarity. The proposed approach is applied to the determination of the engineering-scale in situ stress of the underground powerhouses of the Three Gorges Project, and the results are compared with those obtained by traditional approaches. The comparison further shows that the proposed method has better accuracy than traditional methods.
Adaptive Cruise Control for Cut-In Scenarios Based on Model Predictive Control Algorithm
Authors: Chen Chongpu , Guo Jianhua , Guo Chong , Chen Chaoyi , Zhang Yao , Wang Jiawei
Published: June 2021

In a cut-in scenario, traditional adaptive cruise control usually cannot effectively identify the cut-in vehicle and respond to it in advance. This paper proposes an adaptive cruise control (ACC) strategy based on the MPC algorithm for cut-in scenarios. A finite state machine (FSM) is designed to manage vehicle control in different cut-in scenarios. For a cut-in scenario, a method to identify and quantify the possibility of cut-in of a vehicle is proposed. At the same time, a safety distance model of the cut-in vehicle is established as the basis for the state transition of the finite state machine. Taking the quantified cut-in possibility of a vehicle as a reference, the model predictive control (MPC) algorithm, which considers the constraints of driving safety and comfort, is used to realize coordinated control of the host vehicle and the cut-in vehicle. Simulink–Carsim simulation studies show that the ACC strategy for a cut-in scenario can effectively identify a cut-in vehicle and quantify the possibility of cut-in of the vehicle. Faced with a cut-in vehicle, the host vehicle using the ACC strategy can brake several seconds early and switch the following target to the cut-in vehicle. Meanwhile, the acceleration and jerk of the host vehicle changes within a reasonable range, which ensures driving safety and comfort.
Performance Changes and Recovery Time in U20 and Older Handball Players after a High-Intensity Sprint Exercise
Authors: Klatt Stefanie , Kossmann Melanie , Hottenrott Laura , Ferrauti Alexander , Fasold Frowin
Published: June 2021

This study used a single bout of repeated high-intensity sprint exercise as a variable to compare the performance and recovery time of handball players within a typical age range for team sport athletes. Two test groups (U20 players, n = 12, mean age = 18 years; senior players, n = 17, mean age = 27 years) were observed during and after their performance of a high-intensity interval exercise consisting of four sets of 6 × 40 m all-out change-of-direction sprints. U20 players outperformed senior players in all sprint sets. The groups’ physiological responses and perceived exertion and stress levels were measured immediately before and after the exercise and also after 24, 48, and 72 h. Repeated measures ANOVAs revealed no interaction effects between age and measurement time points on jump height, muscle soreness, and perceived stress levels after the high-intensity interval exercise. However, the U20 athletes showed marginally, but not statistically significant lower creatine kinase (CK) values than the seniors 72 h after the exercise. The vagal heart rate variability (HRV) parameter rMSSD indicated a faster recovery for the U20 athletes compared to the senior players 24 h after the sprint intervals. Overall, the results demonstrate that repeated sprint intervals do not differently affect the physical performance ability (i.e., jump height) of U20 and senior players. Single parameters related to the players’ ability to recover, such as CK and HRV values, show some variations as a function of age. Based on this, coaches may want to consider a longer recovery period after a high-intensity exercise for senior players compared to younger ones.
The Effects of Plasma-Activated Water on Heavy Metals Accumulation in Water Spinach
Authors: Hou Chih-Yao , Kong Ting-Khai , Lin Chia-Min , Chen Hsiu-Ling
Published: June 2021

Toxic heavy metals accumulate in crops from the environment through different routes and may interfere with biochemical reactions in humans, causing serious health consequences. Plasma technology has been assessed for the promotion of seed germination and plant growth in several past studies. Therefore, the aim of the present study was to evaluate whether the growth rate of plants can be increased with the application of non-thermal plasma, as well as to reduce the accumulation of heavy metals in leafy vegetables (water spinach). In this study, several kinds of plasma treatments were applied, such as treatment on the seeds (PTS + NTW), irrigation water (NTS + PAW) or both (PTS + PAW). The results of the study showed that the heavy metals accumulated in water spinach were affected by the heavy metals available in the soil. The bioconcentration factor (BCF) of Cd in water spinach decreased from 0.864 to 0.543 after plasma treatment in seed or irrigating water, while the BCF of Pb was low and did not show any significant changes. Therefore, the results suggest that plasma treatment may suppress Cd absorption, but not for Pb. In this study, plasma treatment did not help to improve the product yield of water spinach planted in Cd-added soil. In the future, fertilizers can be used to supply nutrients that are not provided by plasma-activated water to support the growth of water spinach.
Statistical Approach to Potentially Enhance the Postbiotication of Gluten-Free Sourdough
Authors: Păcularu-Burada Bogdan , Turturică Mihaela , Rocha João Miguel , Bahrim Gabriela-Elena
Published: June 2021

Fermented products are permanently under the attention of scientists and consumers, both due to nutritional importance and health promoting effects. The fermented functional foods contribute to a more balanced diet and increase the immune responses (among many other health effects) with positive implications for quality of life. In this sense, improving the sourdough’s fermentation to boost the biotic (postbiotic and paraprobiotic) properties of the sourdough-based products has positive impacts on the nutritional and functional properties of the final baked products. These enhanced sourdoughs can be obtained in controlled fermentation conditions and used as sourdough bread improvers or novel bioingredients. In this context, our work aimed to optimize, using statistical tools, a gluten-free sourdough based on chickpea, quinoa, and buckwheat fermentation with selected lactic acid bacteria (LAB) to enhance its postbiotic properties. The most important biotechnological parameters were selected by Plackett–Burman Design (PBD) and then Response Surface Methodology (RSM) was applied to evaluate the interactions between the selected factors to maximize the gluten-free sourdough’s properties. As a result, the optimized fermented sourdough had antimicrobial activity with inhibition ratios between 71 and 100% against the Aspergillus niger, Aspergillus flavus, Penicillium spp. molds and against the Bacillus spp endospore-forming Gram-positive rods. The optimized variant showed a total titratable acidity (TTA) of 40.2 mL NaOH 0.1N. Finally, the high-performance liquid chromatography (HPLC) analysis highlighted a heterofermentative profile for the organic acids from the optimized sourdough. Among flavonoids and polyphenols, the level of caffeic and vanillic acids increased after lactic acid fermentation. The comparison between the optimized sourdough and the control evidenced significant differences in the metabolite profiles, thus highlighting its potential postbiotication effect.
Analytic Representation of Maxwell—Boltzmann and Tsallis Thermonuclear Functions with Depleted Tail
Authors: Kumar Dilip , Haubold Hans
Published: June 2021

The closed forms of the non-resonant thermonuclear function in the Maxwell–Boltzmann and Tsallis case with depleted tail are obtained in generalized special functions. The results are written in terms of H-function of two variables. The importance of the results in this paper lies in the fact that the reaction rate probability integrals in Maxwell-Boltzmann and Tsallis cases are not obtained by the conventional method of approximation or by means of a single variable transform technique but by means of a two variable transform method. The behaviour of the depleted non-resonant thermonuclear functions are examined using graphs. The results in the paper are of much interest to astrophysicists and statisticians in their future work in this area.