bluuebunny/crossref_metadata_2025 huggingface dataset:
Oxidative Stress-Induced Misfolding and Inclusion Formation of Nrf2 and Keap1
Authors: Ngo Vy , Karunatilleke Nadun C. , Brickenden Anne , Choy Wing-Yiu , Duennwald Martin L.
Published: January 2022

Cells that experience high levels of oxidative stress respond by inducing antioxidant proteins through activation of the protein transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2). Nrf2 is negatively regulated by the E3 ubiquitin ligase Kelch-like ECH-associated protein 1 (Keap1), which binds to Nrf2 to facilitate its ubiquitination and ensuing proteasomal degradation under basal conditions. Here, we studied protein folding and misfolding in Nrf2 and Keap1 in yeast, mammalian cells, and purified proteins under oxidative stress conditions. Both Nrf2 and Keap1 are susceptible to protein misfolding and inclusion formation upon oxidative stress. We propose that the intrinsically disordered regions within Nrf2 and the high cysteine content of Keap1 contribute to their oxidation and the ensuing misfolding. Our work reveals previously unexplored aspects of Nrf2 and Keap1 regulation and/or dysregulation by oxidation-induced protein misfolding.
Salinity Induced Antioxidant Defense in Roots of Industrial Hemp (IH: Cannabis sativa L.) for Fiber during Seed Germination
Authors: Dixit Naveen
Published: January 2022

Global climate change induced sea level rise, rainfed agriculture, poor quality irrigation water, and seawater intrusion through interconnected ditches and inland waterways cause soil salinity in inland and coastal areas. To reclaim and prevent further soil erosion, salt tolerant crops are required. Industrial Hemp (IH: Cannabis sativa L.) is used for food, fiber, and medicinal purposes throughout the world. In spite of that, little is known about the salt tolerance mechanisms in IH. Seed germination and development of the roots are the primary events in the life cycle of a plant, which directly interact with soil salinity. Therefore, in vitro germination experiments were conducted on the roots of 5-day-old seedlings using four varieties (V1: CFX-2, V2: Joey, V3: Bialobrzeskie, and V4: Henola) of IH for fiber. Five salinity treatments (0, 50, 80, 100, 150, and 200 mM NaCl) were used to screen the IH varieties on the basis of I: seed germination percent (SGP), II: quantitative morphological observations (root length (RL) and root fresh weight (RFW)), III: oxidative stress indices (hydrogen peroxide (H2O2) and lipid peroxidation), and IV: antioxidant defense system comprises of superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPOD), ascorbate peroxidase (APOD), glutathione reductase (GR). The varieties V1 and V3 showed salt tolerance up to 100 mM by maintaining higher SGP, less reduction in RL and RFW. These roots in V1 and V3 showed lower levels of H2O2 and lipid peroxidation by displaying higher activities of SOD, CAT, GPOD, APOD, and GR while a reciprocal trend was observed in V4. However, roots in V2 showed higher activities of antioxidant enzymes with lower levels of H2O2 and lipid peroxidation, but showed declines in RL and RFW at 80 mM NaCl onward. Roots in V4 were the most susceptible to NaCl stress at 50 mM and onward.
Vitamin C Status in People with Types 1 and 2 Diabetes Mellitus and Varying Degrees of Renal Dysfunction: Relationship to Body Weight
Authors: Carr Anitra C. , Spencer Emma , Heenan Helen , Lunt Helen , Vollebregt Monica , Prickett Timothy C. R.
Published: January 2022

Diabetes mellitus is a chronic metabolic disorder and is associated with depleted vitamin C status. The underlying aetiologies and pathogeneses responsible for this association are poorly understood. This retrospective study explored the vitamin C status of 136 adult outpatients with types 1 and 2 diabetes mellitus (T1DM/T2DM), with a focus on indices of renal function and metabolic health, including body weight. In the T1DM group (n = 73), the median plasma vitamin C concentration was 33 (18, 48) µmol/L, with 37% hypovitaminosis C and 12% deficiency. In the T2DM group (n = 63), the median plasma concentration was 15 (7, 29) µmol/L, with 68% hypovitaminosis C and 38% deficiency. Lower vitamin C was associated with macroalbuminuria (p = 0.03), renal dysfunction (p = 0.08), and hypertension (p = 0.0005). Inverse associations were also observed between plasma vitamin C and various other metabolic health parameters (p < 0.05), especially body weight (p < 0.0001), which was higher in those with hypovitaminosis C (<23 µmol/L; p = 0.0001). The association with bodyweight remained, even after multivariable analysis. In summary, body weight was a significant predictor of low vitamin C status in people with diabetes. This suggests that people with both diabetes and a high body weight may have greater than average vitamin C requirements.
Novel Series of Dual NRF2 Inducers and Selective MAO-B Inhibitors for the Treatment of Parkinson’s Disease
Authors: Duarte Pablo , Michalska Patrycja , Crisman Enrique , Cuadrado Antonio , León Rafael
Published: January 2022

Parkinson’s disease (PD) is the second most prevalent neurodegenerative disease. It is characterized by a complex network of physiopathological events where oxidative stress plays a central role among other factors such as neuroinflammation and protein homeostasis. Nuclear factor-erythroid 2 p45-related factor 2 (NRF2) has a multitarget profile itself as it controls a plethora of cellular processes involved in the progression of the disease. In this line, we designed a novel family of 2-(1H-indol-3-yl)ethan-1-amine derivatives as NRF2 inducers with complementary activities. Novel compounds are based on melatonin scaffold and include, among other properties, selective monoamine oxidase B (MAO-B) inhibition activity. Novel multitarget compounds exhibited NRF2 induction activity and MAO-B selective inhibition, combined with anti-inflammatory, antioxidant, and blood–brain barrier permeation properties. Furthermore, they exert neuroprotective properties against oxidative stress toxicity in PD-related in vitro. Hit compound 14 reduced oxidative stress markers and exerted neuroprotection in rat striatal slices exposed to 6-hydroxydopamine or rotenone. In conclusion, we developed a promising family of dual NRF2 inducers and selective MAO-B inhibitors that could serve as a novel therapeutic strategy for PD treatment.
Research on Device Modeling Technique Based on MLP Neural Network for Model Parameter Extraction
Authors: Kang Haixia , Wu Yuping , Chen Lan , Zhang Xuelian
Published: January 2022

The parameter extraction of device models is critically important for circuit simulation. The device models in the existing parameter extraction software are physics-based analytical models, or embedded Simulation program with integrated circuit emphasis (SPICE) functions. The programming implementation of physics-based analytical models is tedious and error prone, while it is time consuming to run the device model evaluation for the device model parameter extraction software by calling the SPICE. We propose a novel modeling technique based on a neural network (NN) for the optimal extraction of device model parameters in this paper, and further integrate the NN model into device model parameter extraction software. The technique does not require developers to understand the device model, which enables faster and less error-prone parameter extraction software developing. Furthermore, the NN model improves the extraction speed compared with the embedded SPICE, which expedites the process of parameter extraction. The technique has been verified on the BSIM-SOI model with a multilayer perceptron (MLP) neural network. The training error of the NN model is 4.14%, and the testing error is 5.38%. Experimental results show that the trained NN model obtains an extraction error of less than 6%, and its extraction speed is thousands of times faster than SPICE in device model parameter extraction.
Economic Energy Allocation of Conventional and Large-Scale PV Power Plants
Authors: El-Hafez Omar Jouma , ElMekkawy Tarek Y. , Kharbeche Mohamed Bin Mokhtar , Massoud Ahmed Mohammed
Published: January 2022

During the past few decades, rapid progress in reducing the cost of photovoltaic (PV) energy has been achieved. At the megawatt (MW) to gigawatt (GW) scale, large PV systems are connected to the electricity grid to provide power during the daytime. Many PVs can be installed on sites with optimal solar radiation and other logistical considerations. However, the electricity produced by the PV power plant has to be transmitted and distributed by the grid, which leads to more power losses. With the widespread commissioning of the large-scale solar PV power plants connected to the grid, it is crucial to have an optimal energy allocation between the conventional and the PV power plants. The electricity cost represents the most significant part of the budget in the power distribution companies, which can reach in many countries billions of dollars. This optimal energy allocation is used to minimize the electricity cost from buyers’ (distribution companies) point of view rather than sellers’ (owners of power plants, i.e., investors) point of view. However, some constraints have to be considered and met, such as water demand, network limitations, and contractual issues such as minimum-take energy. This paper develops a model for the economic energy allocation of conventional and large-scale PV power plants, which considers both the operational aspects and the contractual provisions. The model can be used either in the design or operation phases to minimize the operating cost. Moreover, the proposed model can be used for budgeting tasks. The developed model is entirely generic and can be used for any country or electricity system regardless of the PV energy contribution. Furthermore, the Al-Karsaah power plant located in Qatar is discussed as a case study to validate the claimed contribution.
High-Q TeO2–Si Hybrid Microring Resonators
Authors: Miarabbas Kiani Khadijeh , Bonneville Dawson B. , Knights Andrew P. , Bradley Jonathan D. B.
Published: January 2022

We present the design and experimental measurement of tellurium oxide-clad silicon microring resonators with internal Q factors of up to 1.5 × 106, corresponding to a propagation loss of 0.42 dB/cm at wavelengths around 1550 nm. This compares to a propagation loss of 3.4 dB/cm for unclad waveguides and 0.97 dB/cm for waveguides clad with SiO2. We compared our experimental results with the Payne–Lacey model describing propagation dominated by sidewall scattering. We conclude that the relative increase in the refractive index of TeO2 reduces scattering sufficiently to account for the low propagation loss. These results, in combination with the promising optical properties of TeO2, provide a further step towards realizing compact, monolithic, and low-loss passive, nonlinear, and rare-earth-doped active integrated photonic devices on a silicon photonic platform.
Spatial-Temporal Heterogeneity in the Deformation and Damage of Rock Samples: Experimental Study Using Digital Image Correlation Analysis
Authors: Yan Fayuan , Qi Chengzhi , Shan Renliang
Published: January 2022

In situ observations and laboratory experiments showed that slow deformation waves widely exist in geomedia under loading conditions. Slow deformation waves’ behavior exhibits some similarities in media ranging from the scale as large as the Earth’s crust to the scale as small as the laboratory test samples. However, the mechanism underlying their generation has not been clarified yet. In this research an experimental study was performed on small-scale red sandstone samples subjected to uniaxial compression at the displacement rates of 0.1, 0.5, and 1 mm/min. Slow deformation waves under different loading rates were analyzed by speckle photography for microscopic characterization combined with the digital image correlation (DIC) technique. The Luders deformation bands were predominantly observed in the flow channels formed at the stage of macro-elastic deformation. The spatial-temporal heterogeneity of the rock sample surface was quantified, and the deformation waves’ propagation velocities under different loading rates were obtained. The linear relationship between the propagation velocities of slow deformation waves and the loading rates was determined. The research findings shed some new light on the evolutionary characteristics of the slow deformation waves.
Vapor-Phase Incorporation of Ge in CZTSe Absorbers for Improved Stability of High-Efficiency Kesterite Solar Cells
Authors: Nowak David , Khonsor Talat , Pareek Devendra , Gütay Levent
Published: January 2022

We report an approach to incorporate Ge into Cu2ZnSnSe4 using GeSe vapor during the selenization step of alloyed metallic precursors. The vapor incorporation slowly begins at T ≈ 480 °C and peaks at 530 °C, resulting in a Ge-based composition shift inside the previously formed kesterite layer. We initially observe the formation of a Ge-rich surface layer that merges into a homogeneous distribution of the incorporated element during the further dwelling stage of the annealing. This approach is very versatile and could be used in many similar fabrication processes for incorporating Ge into CZTSe-absorber layers. Because the vapor-based composition shift in the layer happens after the formation of the absorber film towards the end of the fabrication process, most process parameters and the precursor structure may not need any significant re-optimization. The careful integration of this step could help to reduce Sn-related deep defects and accompanying VOC losses. The best CZTGSe-power-conversion efficiency obtained in this series is 10.4 % (with EG = 1.22 eV, FF = 54%, JSC = 36 mA/cm2, VOC = 540 mV, VOCdef,SQ = 417 mV). These results demonstrate the potential of this approach for Ge incorporation into kesterite absorbers.
Use of Lactobacillus plantarum in Preventing Clinical Cases of American and European Foulbrood in Central Italy
Authors: Pietropaoli Marco , Carpana Emanuele , Milito Marcella , Palazzetti Massimo , Guarducci Marcella , Croppi Sofia , Formato Giovanni
Published: January 2022

American and European Foulbrood (AFB and EFB) are considered the most contagious infectious diseases affecting honeybees worldwide. New sustainable strategies need to be implemented for their prevention and control, and probiotics may represent one solution to investigate. In our study, we evaluated the efficacy of one strain of Lactobacillus plantarum (L. plantarum) isolated from northern Italy, orally administered to the bees for AFB and EFB prevention. From March to September 2014, a total of 979 honeybee colonies (9.6% of Viterbo province—Central Italy) were taken under observation from 22 apiaries. Overall prevalence of AFB was 5.3% in treated colonies and 5.1% in the untreated ones. On the contrary, EFB prevalence was lower in the treated colonies (2.5%) compared to the untreated ones (4.5%). L. plantarum showed a significant effect in reducing insurgence of cases of EFB up to 35 days after the end of the treatment (p-value: 0.034). Thanks to this study we could investigate the preventive efficacy of L. plantarum in controlling AFB and EFB, and obtain official data on their clinical prevalence in Central Italy.
Oligomannose-Type Glycan Processing in the Endoplasmic Reticulum and Its Importance in Misfolding Diseases
Authors: Kuribara Taiki , Totani Kiichiro
Published: January 2022

Glycoprotein folding plays a critical role in sorting glycoprotein secretion and degradation in the endoplasmic reticulum (ER). Furthermore, relationships between glycoprotein folding and several diseases, such as type 2 diabetes and various neurodegenerative disorders, are indicated. Patients’ cells with type 2 diabetes, and various neurodegenerative disorders induce ER stress, against which the cells utilize the unfolded protein response for protection. However, in some cases, chronic and/or massive ER stress causes critical damage to cells, leading to the onset of ER stress-related diseases, which are categorized into misfolding diseases. Accumulation of misfolded proteins may be a cause of ER stress, in this respect, perturbation of oligomannose-type glycan processing in the ER may occur. A great number of studies indicate the relationships between ER stress and misfolding diseases, while little evidence has been reported on the connection between oligomannose-type glycan processing and misfolding diseases. In this review, we summarize alteration of oligomannose-type glycan processing in several ER stress-related diseases, especially misfolding diseases and show the possibility of these alteration of oligomannose-type glycan processing as indicators of diseases.
Effects of ω-3 PUFA-Rich Oil Supplementation on Cardiovascular Morphology and Aortic Vascular Reactivity of Adult Male Rats Submitted to an Hypercholesterolemic Diet
Authors: Mendes Furtado Mariely , Lima Rocha Joana Érica , da Silva Mendes Ana Victória , Mello Neto Renato Sampaio , Brito Ana Karolinne da Silva , Sena de Almeida José Otávio Carvalho , Rodrigues Queiroz Emerson Iuri , de Sousa França José Vinícius , Cunha Sales Ana Lina de Carvalho , Gomes Vasconcelos Andreanne , Felix Cabral Wanessa , de Oliveira Lopes Luana , Souza do Carmo Iolanda , Souza Kückelhaus Selma Aparecida , de Souza de Almeida Leite José Roberto , Nunes Adriana Maria Viana , Rizzo Marcia dos Santos , Citó Antônia Maria das Graças Lopes , Fortes Lustosa Ana Karina Marques , Lucarini Massimo , Durazzo Alessandra , Martins Maria do Carmo de Carvalho e , Arcanjo Daniel Dias Rufino
Published: January 2022

Atherosclerosis is a cardiovascular disease associated with abnormalities of vascular functions. The consumption of mono- and polyunsaturated fatty acids can be considered a strategy to reduce clinical events related to atherosclerosis. In the present study, we investigated the effects of supplementation with 310 mg of ω-3 PUFAs (2:1 eicosapentaenoic/docosahexaenoic acids) for 56 days on rats with hypercholesterolemia induced by a diet containing cholesterol (0.1%), cholic acid (0.5%), and egg yolk. Serum biochemical parameters were determined by the enzymatic colorimetric method. Assessment of vascular effects was performed by analysis of histological sections of the heart and aortic arch stained with hematoxylin and eosin and vascular reactivity of the aorta artery. We observed that treatment with ω-3 PUFAs did not promote alterations in lipid profile. On the other hand, we documented a favorable reduction in liver biomarkers, as well as contributions to the preservation of heart and aortic arch morphologies. Interestingly, the vascular reactivity of rat thoracic aortic preparations was improved after treatment with ω-3 PUFAs, with a decrease in hyperreactivity to phenylephrine and increased vasorelaxation promoted by acetylcholine. Our findings suggest that the supplementation of hypercholesterolemic rats with ω-3 PUFAs promoted improvement in liver and vascular endothelial function as well as preserving heart and aortic tissue, reinforcing the early health benefits of ω-3 PUFAs in the development of atherosclerotic plaque and further related events.
Genetic Diversity, Admixture and Analysis of Homozygous-by-Descent (HBD) Segments of Russian Wild Boar
Authors: Kostyunina Olga , Traspov Aleksei , Economov Alexander , Seryodkin Ivan , Senchik Aleksandr , Bakoev Neckruz , Prytkov Yuri , Bardukov Nikolay , Domsky Igor , Karpushkina Tatiana
Published: January 2022

The wild boar is the wild ancestor of the domestic pig and one of the most common species of ungulates. At the beginning of the 20th century, the wild boar was practically exterminated in the European part of Russia. In the period 1935–1988, 7705 boars were caught in various regions of the European part of Russia, the Far East, Ukraine, Belarus, Kyrgyzstan, Kazakhstan, Latvia, Lithuania, Estonia, Tajikistan and resettled in the territory of Russia. Asian and European wild boars dwell the territory of Russia. The aim of our research was to study the genetic diversity and structure of wild boar populations in different regions of Russia using genome-wide genotyping. We have determined the genetic distances, population structure, parameters of genetic diversity and significantly expanded our understanding of the genetic state of the Russian wild boar. For the first time, we calculated autozygosity of the wild boar of the European and Asian subspecies using Homozygous-by-Descent (HBD) Segments analysis, which is important in terms of population recovery. We also found evidence of hybridization between Russian wild boar and domestic pigs. A group of European wild boars showed introgression of the Asian boar into population. The mean level of the inbreeding coefficient in European wild boar was higher than in Asian wild boar, and combined groups of the European boar had higher inbreeding coefficient than Russian wild boars. These results obtained can be used in population management.
Coastal Mesozooplankton Assemblages during Spring Bloom in the Eastern Barents Sea
Authors: Dvoretsky Vladimir G. , Dvoretsky Alexander G.
Published: January 2022

Mesozooplankton play an important role in Arctic shelf ecosystems as a trophic link and a key food source for many larval fish species. The distribution of mesozooplankton in the eastern Barents Sea was studied along a 500 nautical mile-long transect in May 2016 during the spring bloom. Mesozooplankton were sampled using a Juday net hauled from the surface to the bottom at 12 stations. We found good correspondence between the distribution of water masses and mesozooplankton assemblages. Mesozooplankton abundance (mean 73·103 individuals m−2) in Novaya Zemlya coastal water was dominated by Copepoda ova and nauplii, Thyssanoessa spp. nauplii and Oithona similis. Parasagitta elegans and Calanus finmarchicus comprised most of the total mesozooplankton biomass (mean 0.9 g dry mass m−2) in that water mass. A second assemblage (mean abundance 171·103 individuals m−2) was associated with the colder Barents Sea water, with Oithona similis, Copepoda nauplii, Fritillaria borealis and Cirripedia nauplii being the most numerous. In that water mass, C. finmarchicus, Metridia longa, Cirripedia nauplii and Calanus glacialis contributed most to the total biomass (mean 3 g dry mass m−2). The dominance of young stages of Copepoda and a high proportion of meroplankton were typical of spring mesozooplankton assemblages. The spatial distribution of mesozooplankton abundance and biomass was strongly correlated with latitude, longitude and chlorophyll a concentration, which together explained 10% of the total variance in mesozooplankton density. The present investigation is a baseline study for the assessment of the spring mesozooplankton assemblage in the eastern Barents Sea, and for an evaluation of the possible impact of future environmental changes on the Arctic shelf marine ecosystem.
Predicting Innovative Work Behaviour in an Interactive Mechanism
Authors: Afrin Samina , Raihan Tarik , Uddin Ahmed Ishmum , Uddin Md. Aftab
Published: January 2022

The purpose of this paper is to investigate the impacts of employees’ creative self-efficacy (CSE) and creative self-identity (CSI) on their innovative work behaviour (IWB), with the indirect effects of creative process engagement (CPE) and creative climate (CC). Following the deductive reasoning approach, the study was conducted on IT-based firms in Bangladesh. A total of 348 surveys were collected using a multi-item questionnaire. The collected data were then analyzed using structural equation modeling (SEM). The study reveals a significant relationship between CSE and IWB, and CSI and IWB. It further explores the significant mediating effects of CPE and the moderating effects of CC on CSE and IWB, and CSI and IB, relationships. Based on the premise of interactionist perspectives on creativity, this study contributes to the literature proposing a distinctive model comprising five variables to investigate employees’ IWB from a multi-level perspective. This integrated model, using predictors from multiple levels, supports the theoretical assumption that IB results from employees’ CSE, CSI, and, finally, CPE. Distinct from the other literature, the study also portrays the moderating and mediating impact of CC and CPE simultaneously.
Out-of-Plane Tensile Properties of Cross Laminated Timber (CLT)
Authors: Brandner Reinhard , Jantscher Lukas
Published: January 2022

A systematic investigation is still lacking for tension out-of-plane in cross laminated timber (CLT), as a planar timber construction product. The objectives of the present study are the determination of the tensile properties of CLT made of Norway spruce, the identification of essential product-specific influencing parameters and a comparative analysis with glulam. For this purpose, seven test series were defined, which allowed the determination of the tensile properties on board segments and thereof produced glulam and CLT specimens by varying the number of layers, layer orientation and number of elements within a layer. The orthogonal laminated structure of CLT led to between 50% and 70% higher tensile properties out-of-plane, which is explained by the different stress distribution compared to glulam; the regulation of 30% higher properties than for glulam is suggested. In addition, the lognormal distribution turned out to be a more representative distribution model for characterizing the tensile strength out-of-plane than the Weibull distribution. This was also confirmed with regard to the investigated serial and parallel system effects, in which a clearly more homogeneous behavior was found in CLT compared to glulam, which in turn can be attributed again to the different stress distributions.
Analyzing the Environment Characteristics of Heat Exposure Spaces from the Humanistic Perspective and Spatial Improvement Approaches in Central Beijing, China
Authors: Yang Xin , Gao Wenwen , Zhang Qi , Li Sha , Fu Fan , Li Nana
Published: January 2022

Global warming, high temperatures, and heatwave weather are some of the factors affecting human settlement environment health. In high-temperature weather, human production and life are seriously threatened, as long-term exposure to high temperatures causes a variety of diseases, and children and elderly, who have poor tolerance, require strengthened protection. From a human perspective, this study calculated the thermal duration distribution of high temperatures based on maximum temperature data in a central urban area of Beijing combined with the results of the sixth population census of Beijing, investigated the population distribution of individuals under 15 years old and over 65 years old, and analyzed the spatial distribution of a thermal exposure space in a central urban area of Beijing with the help of the ArcGIS platform. Based on 130 district districts, streets with high-risk heat exposure spaces in the central urban area of Beijing were reddened to determine the distribution of high-risk grades. Using the semantic segmentation method and a street view map, the high-risk thermal exposure space environment from the humanistic perspective was restored, and the typical characteristics were summarized and analyzed. Finally, the environmental characteristics of the high-risk thermal exposure space were analyzed from the humanistic perspective, and an improvement strategy for thermal exposure spaces was proposed based on the perspective of emotional relief.
Clinical Value of Surveillance 18F-fluorodeoxyglucose PET/CT for Detecting Unsuspected Recurrence or Second Primary Cancer in Non-Small Cell Lung Cancer after Curative Therapy
Authors: Lim Chae Hong , Park Soo Bin , Kim Hong Kwan , Choi Yong Soo , Kim Jhingook , Ahn Yong Chan , Ahn Myung-ju , Choi Joon Young
Published: January 2022

We evaluated the diagnostic value of 18F-fluorodeoxyglucose (FDG) positron emission tomography (PET)/CT surveillance for detecting clinically unsuspected recurrence or second primary cancer (SPC) in patients with non-small cell lung cancer (NSCLC) after curative therapy. A total of 4478 surveillance FDG PET/CT scans from 2864 NSCLC patients without suspicion of recurrence after curative therapy were reviewed retrospectively. In 274 of 2864 (9.6%) patients, recurrent NSCLC or SPC was found by surveillance PET/CT during clinical follow-up. Surveillance PET/CT scans showed sensitivity of 98.9% (274/277), specificity of 98.1% (4122/4201), accuracy of 98.2% (4396/4478), positive predictive value (PPV) of 77.6% (274/353), and negative predictive value of 99.9% (4122/4125). The specificity and accuracy in the curative surgery group were significantly higher than those in the curative radiotherapy group. PPV was significantly improved in subgroups of patients with advanced stage prior to curative therapy, PET/CT scans performed within 3 years after curative-intent therapy, and curative surgery. FDG PET/CT surveillance showed good diagnostic efficacy for detecting clinically unexpected recurrence or SPC in NSCLC patients after curative therapy. It can be more useful when performed soon after therapy in curative surgery recipients and those with an advanced disease stage considering its diagnostic efficacy and yield.
Study on Catalytic Water Oxidation Properties of Polynuclear Manganese Containing Polyoxometalates
Authors: Wu Yue , Pei Jianye , Yu Xiaoxia , Bi Lihua
Published: January 2022

Splitting of water to produce hydrogen and oxygen is a green and effective method to produce clean energy. Finding an efficient water decomposition catalyst is the key step to realize water decomposition. In this work, by choosing from the literature, six polynuclear manganese (Mn) containing polyoxometalates (Mn-POMs) with different Mn-O clusters and oxidation states of Mn, [MnIIMnIIISiW10O37(OH)(H2O)]6− (Mn2-POM), [MnII3MnIII(H2O)2(PW9O34)2]9− (Mn4-POM), [MnII4MnIII2Ge3W24O94(H2O)2]18− (Mn6-POM-1), [MnIII2MnII4(μ3-O)2(H2O)4(B-β-SiW8O31)(B-β-SiW9O34)(γ-SiW10O36)]18− (Mn6-POM-4), [{MnIII3MnIV4O4(OH)2(OH2)}2(W6O22)(H2W8O32)2(H4W13O46)2]26− (Mn14-POM), [MnII19 (OH)12(SiW10O37)6]34− (Mn19-POM) were prepared. First, the catalytic performance towards the water oxidation of six Mn-POMs was investigated in solution for the first time. Second, six Mn-POMs were fabricated on the surface of ITO electrode using layer-by-layer self-assembly (LBL) to form the composite films, which were characterized by UV-vis spectroscopy and cyclic voltammetry, and then the catalytic water oxidation performance of the composite films was studied and compared with that in solution via a series of controlled experiments, the results indicate that the Mn-POMs with three-dimensional structures, which contain variable valence Mn-O cluster similar to the structure of photocatalytic active center (PSII) exhibit better catalytic performance.
Acrylonitrile and Pullulan Based Nanofiber Mats as Easily Accessible Scaffolds for 3D Skin Cell Models Containing Primary Cells
Authors: Rimann Markus , Jüngel Astrid , Mousavi Sara , Moeschlin Nicole , Calcagni Maurizio , Wuertz-Kozak Karin , Brunner Florian , Dudli Stefan , Distler Oliver , Adlhart Christian
Published: January 2022

(1) Background: Three-dimensional (3D) collagen I-based skin models are commonly used in drug development and substance testing but have major drawbacks such as batch-to-batch variations and ethical concerns. Recently, synthetic nanofibrous scaffolds created by electrospinning have received increasing interest as potential alternatives due to their morphological similarities to native collagen fibrils in size and orientation. The overall objective of this proof-of-concept study was to demonstrate the suitability of two synthetic polymers in creating electrospun scaffolds for 3D skin cell models. (2) Methods: Electrospun nanofiber mats were produced with (i) poly(acrylonitrile-co-methyl acrylate) (P(AN-MA)) and (ii) a blend of pullulan (Pul), poly(vinyl alcohol) (PVA) and poly(acrylic acid) (PAA) (Pul/PVA/PAA) and characterized by scanning electron microscopy (SEM) and diffuse reflectance infrared Fourier transform (DRIFT) spectra. Primary skin fibroblasts and keratinocytes were seeded onto the nanofiber mats and analyzed for phenotypic characteristics (phalloidin staining), viability (Presto Blue HS assay), proliferation (Ki-67 staining), distribution (H/E staining), responsiveness to biological stimuli (qPCR), and formation of skin-like structures (H/E staining). (3) Results: P(AN-MA) mats were more loosely packed than the Pul/PVA/PAA mats, concomitant with larger fiber diameter (340 nm ± 120 nm vs. 250 nm ± 120 nm, p < 0.0001). After sterilization and exposure to cell culture media for 28 days, P(AN-MA) mats showed significant adsorption of fetal calf serum (FCS) from the media into the fibers (DRIFT spectra) and increased fiber diameter (590 nm ± 290 nm, p < 0.0001). Skin fibroblasts were viable over time on both nanofiber mats, but suitable cell infiltration only occurred in the P(AN-MA) nanofiber mats. On P(AN-MA) mats, fibroblasts showed their characteristic spindle-like shape, produced a dermis-like structure, and responded well to TGFβ stimulation, with a significant increase in the mRNA expression of PAI1, COL1A1, and αSMA (all p < 0.05). Primary keratinocytes seeded on top of the dermis equivalent proliferated and formed a stratified epidermis-like structure. (4) Conclusion: P(AN-MA) and Pul/PVA/PAA are both biocompatible materials suitable for nanofiber mat production. P(AN-MA) mats hold greater potential as future 3D skin models due to enhanced cell compatibility (i.e., adsorption of FCS proteins), cell infiltration (i.e., increased pore size due to swelling behavior), and cell phenotype preservation. Thus, our proof-of-concept study shows an easy and robust process of producing electrospun scaffolds for 3D skin cell models made of P(AN-MA) nanofibers without the need for bioactive molecule attachments.
Spatial Proteomic Analysis of Isogenic Metastatic Colorectal Cancer Cells Reveals Key Dysregulated Proteins Associated with Lymph Node, Liver, and Lung Metastasis
Authors: Solís-Fernández Guillermo , Montero-Calle Ana , Martínez-Useros Javier , López-Janeiro Álvaro , de los Ríos Vivian , Sanz Rodrigo , Dziakova Jana , Milagrosa Elena , Fernández-Aceñero María Jesús , Peláez-García Alberto , Casal José Ignacio , Hofkens Johan , Rocha Susana , Barderas Rodrigo
Published: January 2022

Metastasis is the primary cause of colorectal cancer (CRC) death. The liver and lung, besides adjacent lymph nodes, are the most common sites of metastasis. Here, we aimed to study the lymph nodes, liver, and lung CRC metastasis by quantitative spatial proteomics analysis using CRC cell-based models that recapitulate these metastases. The isogenic KM12 cell system composed of the non-metastatic KM12C cells, liver metastatic KM12SM cells, and liver and lung metastatic KM12L4a cells, and the isogenic non-metastatic SW480 and lymph nodes metastatic SW620 cells, were used. Cells were fractionated to study by proteomics five subcellular fractions corresponding to cytoplasm, membrane, nucleus, chromatin-bound proteins, and cytoskeletal proteins, and the secretome. Trypsin digested extracts were labeled with TMT 11-plex and fractionated prior to proteomics analysis on a Q Exactive. We provide data on protein abundance and localization of 4710 proteins in their different subcellular fractions, depicting dysregulation of proteins in abundance and/or localization in the most common sites of CRC metastasis. After bioinformatics, alterations in abundance and localization for selected proteins from diverse subcellular localizations were validated via WB, IF, IHC, and ELISA using CRC cells, patient tissues, and plasma samples. Results supported the relevance of the proteomics results in an actual CRC scenario. It was particularly relevant that the measurement of GLG1 in plasma showed diagnostic ability of advanced stages of the disease, and that the mislocalization of MUC5AC and BAIAP2 in the nucleus and membrane, respectively, was significantly associated with poor prognosis of CRC patients. Our results demonstrate that the analysis of cell extracts dilutes protein alterations in abundance in specific localizations that might only be observed studying specific subcellular fractions, as here observed for BAIAP2, GLG1, PHYHIPL, TNFRSF10A, or CDKN2AIP, which are interesting proteins that should be further analyzed in CRC metastasis.
No Difference in the Incidence of Complications in Pediatric Patients with Moderate Anemia 30 Days after Pediatric Hip Surgery with and without Blood Transfusion
Authors: Chutarattanakul Phasuth , Kaewpornsawan Kamolporn , Wongcharoenwatana Jidapa , Musikachart Piyanuch , Eamsobhana Perajit
Published: January 2022

This study investigated the association between postoperative blood transfusion and the incidence of postoperative complications 30 days after pediatric hip surgery as well as factors significantly associated with 30-day postoperative complications. Patients were divided into two groups: those with postoperative complications and those with no complications. Postoperative hematocrit (Hct) was categorized as <25%, 25–30%, and >30%. Comparison was made between all postoperative complications at the 30-day follow-up that were influenced by anemia in patients who received transfusion and those who did not. A multivariate logistic regression model was used to identify factors independently associated with postoperative complications. The overall 30-day postoperative complication rate for all patients was 17% (24/138). No significant difference between the transfusion and the non-transfusion patients was found. Preoperative hematocrit (Hct) was significantly lower in the complications group (p = 0.030), and both length of stay and 30-day readmission were significantly higher in patients with complications (p = 0.011 and p < 0.001, respectively). Multivariate analysis revealed female gender (OR: 3.50, 95% CI: 1.18–10.36; p = 0.026) and length of hospital stay (OR: 1.23, 95% CI: 1.08–1.41; p = 0.004) to be factors independently associated with 30-day postoperative complications. However, no statistically significant difference in the incidence of complications at 30 days following pediatric hip dysplasia surgery was found between patients who received blood transfusion to maintain a Hct level ≥25% and those not receiving transfusion.
Developing Biliary Atresia-like Model by Treating Human Liver Organoids with Polyinosinic:Polycytidylic Acid (Poly (I:C))
Authors: Chung Patrick Ho-Yu , Babu Rosana Ottakandathil , Wu Zhongluan , Wong Kenneth Kak-Yuen , Tam Paul Kwong-Hang , Lui Vincent Chi-Hang
Published: January 2022

Background: We explored the feasibility of creating BA-like organoids by treating human liver organoids with Polyinosinic:Polycytidylic acid (Poly I:C). Methods: Organoids were developed from the liver parenchyma collected during Kasai portoenterostomy (BA) and surgery for other liver disorders (non-BA). The non-BA organoids were co-cultured with poly I:C (40 µg/mL). The organoid morphology from both samples was compared on day 17. RNA-sequencing was performed to examine the transcriptomic differences. Results: Non-BA liver organoids developed into well-expanded spherical organoids with a single-cell layer of epithelial cells and a single vacuole inside. After poly I:C treatment, the majority of these organoids developed into an aberrant morphology with a high index of similarity to BA organoids which are multi-vacuoled and/or unexpanded. RNA-sequencing analysis revealed that 19 inflammatory genes were commonly expressed in both groups. Conditional cluster analysis revealed several genes (SOCS6, SOCS6.1, ARAF, CAMK2G, GNA1C, ITGA2, PRKACA, PTEN) that are involved in immune-mediated signaling pathway had a distinct pattern of expression in the poly I:C treated organoids. This resembled the expression pattern in BA organoids (p < 0.05). Conclusions: Poly I:C treated human liver organoids exhibit morphology and genetic signature highly compatible to organoids developed from BA liver samples. They are potential research materials to study immune-mediated inflammation in BA.
A Fault Feature Extraction Method Based on LMD and Wavelet Packet Denoising
Authors: Yang Jingzong , Zhou Chengjiang
Published: January 2022

Aiming at the problem of fault feature extraction of a diaphragm pump check valve, a fault feature extraction method based on local mean decomposition (LMD) and wavelet packet transform is proposed. Firstly, the collected vibration signal was decomposed by LMD. After several amplitude modulation (AM) and frequency modulation (FM) components were obtained, the effective components were selected according to the Kullback-Leible (K-L) divergence of all component signals for reconstruction. Then, wavelet packet transform was used to denoise the reconstructed signal. Finally, the characteristics of the fault signal were extracted by Hilbert envelope spectrum analysis. Through experimental analysis, the results show that compared with other traditional methods, the proposed method can effectively overcome the phenomenon of mode aliasing and extract the fault characteristics of a check valve more effectively. Experiments show that this method is feasible in the fault diagnosis of check valve.
Deposition of CdZnTe Films with CSS Method on Different Substrates for Nuclear Radiation Detector Applications
Authors: Yu Bin , Xu Chenggang , Xie Mingxing , Cao Meng , Zhang Jijun , Jiang Yucheng , Wang Linjun
Published: January 2022

CdZnTe (CZT) films were grown by closed space sublimation (CSS) method on (111)-oriented CZT wafers, non-oriented CZT wafers and FTO substrates. The compositional and morphological properties of CZT films on different substrates were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM), which indicated that CZT films grown on (111)-oriented CZT wafers had low dislocation density and high Zn composition. X-ray diffraction (XRD) measurements confirmed that CZT films grown on (111)-oriented CZT wafers had the best crystal quality. The I-V and DC photoconductivity measurements indicated that CZT films on (111)-oriented CZT wafer had good carrier transport performance. The energy spectra of CZT films grown on (111)-oriented CZT wafer presented that it had a good response to the nuclear radiation under 241Am.
Semi-Automated Quantification of Retinal and Choroidal Biomarkers in Retinal Vascular Diseases: Agreement of Spectral-Domain Optical Coherence Tomography with and without Enhanced Depth Imaging Mode
Authors: Kessler Lucy J. , Bagautdinov Dmitrii , Łabuz Grzegorz , Auffarth Gerd U. , Khoramnia Ramin
Published: January 2022

Background: We compared with and without enhanced depth imaging mode (EDI) in semi-automated quantification of retinal and choroidal biomarkers in optical coherence tomography (OCT) in patients with diabetic retinopathy (DR) or retinal vein occlusion (RVO) complicated by macular edema. We chose to study three OCT biomarkers: the numbers of hyperreflective foci (HF), the ellipsoid zone reflectivity ratio (EZR) and the choroidal vascularity index (CVI), all known to be correlated with visual acuity changes or treatment outcomes. Methods: In a single examination, one eye of each patient (n = 60; diabetic retinopathy: n = 27, retinal vein occlusion: n = 33) underwent macular 870 nm spectral domain-OCT (SD-OCT) B-scans without and with EDI mode. Semi-automated quantification of HF, EZR and CVI was applied according to preexisting published protocols. Paired Student’s t-test or Wilcoxon rank-sum test was used to test for differences in subgroups. Intraclass correlation coefficient (ICC) and Bland–Altman plots were applied to describe the agreement between quantification in EDI and conventional OCT mode. The effect of macular edema on semi-automated quantification was evaluated. Results: For the entire cohort, quantification of all three biomarkers was not significantly different in SD-OCT scans with and without EDI mode (p > 0.05). ICC was 0.78, 0.90 and 0.80 for HF, EZR and CVI. The presence of macular edema led to significant differences in the quantification of hyperreflective foci (without EDI: 80.00 ± 33.70, with EDI: 92.08 ± 38.11; mean difference: 12.09, p = 0.03), but not in the quantification of EZR and CVI (p > 0.05). Conclusion: Quantification of EZR and CVI was comparable whether or not EDI mode was used. In conclusion, both retinal and choroidal biomarkers can be quantified from one single 870 nm SD-OCT EDI image.
The Use of Intact Fish Skin as a Novel Treatment Method for Deep Dermal Burns Following Enzymatic Debridement: A Retrospective Case-Control Study
Authors: Wallner Christoph , Holtermann Jana , Drysch Marius , Schmidt Sonja , Reinkemeier Felix , Wagner Johannes Maximilian , Dadras Mehran , Sogorski Alexander , Houschyar Khosrow Siamak , Becerikli Mustafa , Lehnhardt Marcus , Behr Björn
Published: January 2022

Background: The optimal therapy for deep burn wounds is based on the early debridement of necrotic tissue followed by wound coverage to avoid a systemic inflammatory response and optimize scar-free healing. The outcomes are affected by available resources and underlying patient factors, which represent challenges in burn care and suboptimal outcomes. In this study, we aimed to determine optimal burn-wound management using enzymatic debridement (NexoBrid™, MediWound Germany GmbH, Rüsselsheim, Germany) and intact fish skin (Kerecis® Omega3 Wound, Isafjordur, Iceland). Methods: In this retrospective case series, 12 patients with superficial or deep dermal burn wounds were treated with enzymatic debridement followed by fish skin, Suprathel® (PolyMedics Innovations GmbH, Denkendorf, Germany), or a split-thickness skin graft (STSG). Patients’ outcomes regarding healing and scar quality were collected objectively and subjectively for 12 months after the burn injury. Results: Wounds treated with fish skin demonstrated accelerated wound healing, a significantly higher water-storage capacity, and better pain relief. Furthermore, improved functional and cosmetic outcomes, such as elasticity, skin thickness, and pigmentation, were demonstrated. The pain and itch expressed as POSAS scores (Patient and Observer Scar Assessment Scale) for fish skin decreased compared to those for wounds managed with an STSG or Suprathel. Importantly, fish skin-treated wounds had significantly improved sebum production and skin elasticity than those treated with Suprathel but showed no significant superiority compared to STSG-treated wounds. Conclusions: Enzymatic debridement in combination with intact fish skin grafts resulted in the faster healing of burn wounds and better functional and aesthetic outcomes than split-thickness skin grafts and Suprathel treatment.
Radiation Qualification by Means of the System-Level Testing: Opportunities and Limitations
Authors: Rajkowski Tomasz , Saigné Frédéric , Wang Pierre-Xiao
Published: January 2022

System-level radiation testing of electronics is evaluated, based on test examples of the System-in-Package (SiP) module irradiations. Total ionizing dose and single event effects tests are analyzed to better understand the opportunities and limitations of the system-level approach in the context of the radiation qualification of electronics. Impact on the SiP product development is discussed.
A Computationally Efficient Model Predictive Current Control of Synchronous Reluctance Motors Based on Hysteresis Comparators
Authors: Benjamim Wagner , Jlassi Imed , Cardoso Antonio J. Marques
Published: January 2022

Model predictive current control (MPCC) has recently become a powerful advanced control technology in industrial drives. However, current prediction in MPCC requires a high number of voltage vectors (VVs) synthesizable by the converter, thus being computationally demanding. Accordingly, in this paper, a computationally efficient MPCC of synchronous reluctance motors (SynRMs) that reduces the number of VVs used for prediction is proposed. By making the most of the simplicity of hysteresis current control (HCC) and integrating it with the MPCC scheme, only four out of eight predictions are needed to determine the best VV, dramatically reducing algorithm computations. The experimental results show that the execution time can be shortened by 20% while maintaining the highest control efficiency.