bluuebunny/crossref_metadata_2025 huggingface dataset:
Electrospun Nanofibers Revisited: An Update on the Emerging Applications in Nanomedicine
Authors: Elsadek Nehal E. , Nagah Abdalrazeq , Ibrahim Tarek M. , Chopra Hitesh , Ghonaim Ghada A. , Emam Sherif E. , Cavalu Simona , Attia Mohamed S.
Published: March 2022

Electrospinning (ES) has become a straightforward and customizable drug delivery technique for fabricating drug-loaded nanofibers (NFs) using various biodegradable and non-biodegradable polymers. One of NF’s pros is to provide a controlled drug release through managing the NF structure by changing the spinneret type and nature of the used polymer. Electrospun NFs are employed as implants in several applications including, cancer therapy, microbial infections, and regenerative medicine. These implants facilitate a unique local delivery of chemotherapy because of their high loading capability, wide surface area, and cost-effectiveness. Multi-drug combination, magnetic, thermal, and gene therapies are promising strategies for improving chemotherapeutic efficiency. In addition, implants are recognized as an effective antimicrobial drug delivery system overriding drawbacks of traditional antibiotic administration routes such as their bioavailability and dosage levels. Recently, a sophisticated strategy has emerged for wound healing by producing biomimetic nanofibrous materials with clinically relevant properties and desirable loading capability with regenerative agents. Electrospun NFs have proposed unique solutions, including pelvic organ prolapse treatment, viable alternatives to surgical operations, and dental tissue regeneration. Conventional ES setups include difficult-assembled mega-sized equipment producing bulky matrices with inadequate stability and storage. Lately, there has become an increasing need for portable ES devices using completely available off-shelf materials to yield highly-efficient NFs for dressing wounds and rapid hemostasis. This review covers recent updates on electrospun NFs in nanomedicine applications. ES of biopolymers and drugs is discussed regarding their current scope and future outlook.
Enhanced Switching Reliability of Sol–Gel-Processed Y2O3 RRAM Devices Based on Y2O3 Surface Roughness-Induced Local Electric Field
Authors: Kim Do-Won , Kim Hyeon-Joong , Lee Won-Yong , Kim Kyoungdu , Lee Sin-Hyung , Bae Jin-Hyuk , Kang In-Man , Kim Kwangeun , Jang Jaewon
Published: March 2022

Sol–gel-processed Y2O3 films were used as active channel layers for resistive random access memory (RRAM) devices. The fabricated ITO/Y2O3/Ag RRAM devices exhibited the properties of conventional bipolar memory devices. A triethylamine stabilizer with a high vapor pressure and low surface tension was added to realize the local electric field area. During drying and high-temperature post-annealing processes, the large convective flow enhanced the surface elevation, and the increased –OH groups accelerated the hydrolysis reaction and aggregation. These phenomena afforded Y2O3 films with an uneven surface morphology and an increased surface roughness. The increased roughness of the Y2O3 films attributable to the triethylamine stabilizer enhanced the local electrical field, improved device reliability, and achieved successful repetition of the switching properties over an extended period.
A Sustainable Technique to Prepare High-Purity Vanadium Pentoxide via Purification with Low Ammonium Consumption
Authors: Lin Guoce , Huang Jing , Zhang Yimin , Hu Pengcheng
Published: March 2022

The general preparation method for V2O5 is ammonium salt vanadium precipitation, which inevitably produces large amounts of ammonia nitrogen wastewater. In this paper, we propose an environmentally friendly method for preparing high-purity V2O5 with low ammonium consumption. The purity of the V2O5 product reaches more than 99% while reducing the level of ammonium consumption. The vanadium precipitation efficiency reaches 99.23% and the V2O5 purity of the product reaches 99.05% under the following conditions: precipitation time of 1.5 h, precipitation temperature of 98 °C, initial precipitation pH of 2, ammonium addition coefficient of 2, purification time of 5 min with purification performed twice, purification temperature of 65 °C. In this study, compared with the use of ammonia spirit for vanadium precipitation and ammonium salt vanadium precipitation, the ammonia consumption levels are reduced by 79.80% and 80.00%, and the purity levels are increased by 0.70% and 1.01%, respectively. The compositions of the precipitated (NaV3O8∙xH2O) and purified ((NH4)2V6O16·1.5H2O) hydrolysis products are characterized via XRD. The TGA results show that NaV3O8∙xH2O contains 1.5 times the amount of crystal water. The FTIR results explain that the two V3O8− layers are combined end-to-end to form a V6O162− layer. The change of the product image indicates that the purification process includes three stages. Firstly, heating and NH4+ attack expand the V3O8− layer. NH4+ diffuses more easily into the V3O8− layer. Secondly, NH4+ destroys the electrostatic interaction between Na+ with the V3O8− layer and replacing Na+. Finally, V3O8− is polymerized into V6O162− to keep the crystal structure stable.
Nanoporous–Crystalline Poly(2,6-dimethyl-1,4-phenylene)oxide Aerogels with Selectively Sulfonated Amorphous Phase for Fast VOC Sorption from Water
Authors: Pellegrino Marina , Fiumarella Adriano , Moretta Alma , Daniel Christophe , Trifuoggi Marco , Borriello Anna , Venditto Vincenzo
Published: March 2022

This paper describes the preparation and characterization of poly(2,6-dimethyl-1,4-phenylene)oxide (PPO) highly porous monolithic aerogels with a hydrophobic nanoporous–crystalline phase and a hydrophilic sulfonated amorphous phase. The sulfonated aerogels were obtained by the sulfonation of PPO physical gels, followed by the supercritical CO2 extraction of solvents. WAXD and FTIR analysis showed that the nanoporous–crystalline phase was preserved for a degree of sulfonation up to c.a. 35%, allowing a highly volatile organic compound (VOC) sorption capacity. The sulfonated PPO aerogels exhibited a high water sorption capacity, with a water uptake of up to 500 wt%, and faster VOC sorption kinetics from water with respect to unsulfonated aerogels.
Experimental Study of Mechanical Properties of Polypropylene Random Copolymer and Rice-Husk-Based Biocomposite by Using Nanoindentation
Authors: Rabbani Fahad Ali , Yasin Saima , Iqbal Tanveer , Farooq Ujala
Published: March 2022

Nanoindentation is widely used to investigate the surface-mechanical properties of biocomposites. In this study, polypropylene random copolymer (PPRC) and biowaste rice husk (BRH) were used as the main raw materials, and glass-fiber-reinforced polypropylene and talc were also used with BRH to enhance the mechanical characterization of the biocomposites. The interfacial bonding between the polymer and the rice husk was increased by treating them with maleic anhydride and NaOH, respectively. The results obtained from the nanoindentation indicated that the plastic behavior of the biocomposites was prominent when untreated BRH was used and vice versa. The modulus and hardness of the biocomposite improved by 44.8% and 54.8% due to the neat PPRC, respectively. The tribological properties were studied based on the hardness-to-modulus ratio and it was found that BRH- and talc-based biocomposites were better than other samples in terms of low friction and wear rate. The creep measurements showed that untreated rice husk biocomposite exhibited high resistance to load deformation.
Impact of Iterative Deuterium Annealing in Long-Channel MOSFET Performance
Authors: Wang Dong-Hyun , Ku Ja-Yun , Jung Dae-Han , Lee Khwang-Sun , Shin Woo Cheol , Yang Byung-Do , Park Jun-Young
Published: March 2022

In contrast to conventional forming gas annealing (FGA), high-pressure deuterium annealing (HPD) shows a superior passivation of dangling bonds on the Si/SiO2 interface. However, research detailing the process optimization for HPD has been modest. In this context, this paper demonstrates the iterative impact of HPD for the better fabrication of semiconductor devices. Long-channel gate-enclosed FETs are fabricated as a test vehicle. After each cycle of the annealing, device parameters are extracted and compared depending on the number of the HPD. Based on the results, an HPD condition that maximizes on-state current (ION) but minimizes off-state current (IOFF) can be provided.
Accuracy Evaluation of Thermoelastic Stress Analysis with the Use of Experimental and Numerical Methods
Authors: Misiewicz Robert , Moczko Przemysław , Bajcar Adam
Published: March 2022

Thermoelastic Stress Analysis (TSA) is one of the very few methods allowing the determination of a continuous stress distribution on the object’s surface under variable loading conditions. Such results provide a lot of valuable information in the field of technical condition assessment and residual life prediction. In order to improve the accuracy of the TSA, the Lock-In signal processing method is implemented. This research is aimed at verifying the effectiveness of this improvement and determining the TSA stress detection threshold, as it is important information in terms of the applicability of this method in the low-stress conditions encountered in considerations of fatigue of load-carrying structures. A steel sample with a centrally located hole was subjected to cyclic loads to determine the threshold of stress detection and accuracy of TSA. As a result of the research, the relationship between the magnitude of stress excitations and the underestimation of the measured stresses was developed. Based on the conducted investigations, it was concluded that reasonable TSA results can be acquired for excitations that induce a temperature response above 10 mK (0.5 NEDT). The presented field test example proves that in industrial applications reasonable results can be acquired for thermal responses below the NEDT of the IR camera. It was concluded that it is possible to successfully implement TSA in low-stress applications (temperature response below NEDT).
Biocompatibility of a New Calcium Silicate-Based Root Canal Sealer Mediated via the Modulation of Macrophage Polarization in a Rat Model
Authors: Yang Xiaoqian , Tian Jun , Li Mengjie , Chen Weiyang , Liu He , Wang Zhejun , Haapasalo Markus , Shen Ya , Wei Xi
Published: March 2022

(1) Background: The EndoSequence BC Sealer HiFlow (Brasseler, Savannah, GA, USA) has recently been introduced in clinical applications. Thus, the aims of the present study are to determine its biocompatibility in vivo and to examine its ability to drive macrophage polarization in vitro and in vivo. (2) Methods: HiFlow was implanted into rat connective tissue for 7, 30 and 150 days. The microstructures and elemental compositions were determined by scanning electron microscopy-energy-dispersive X-ray spectroscopy (SEM-EDX). Hematoxylin–eosin, immunofluorescence, RT–qPCR and flow cytometry were used to elucidate the effects on inflammatory responses and macrophage polarization. (3) Results: SEM-EDX revealed the formation of surface hydroxyapatite crystal layers. Histological evaluation showed that HiFlow exhibited long-term biocompatibility because it decreased inflammatory responses and reduced the number of macrophages over time; however, tissue necrosis was observed in all the groups. RT–qPCR verified that HiFlow regulated the expression of inflammatory factors to inhibit the inflammatory response. Immunofluorescence analysis performed on in vivo samples revealed that HiFlow promoted M2-like macrophage polarization, and these results were confirmed by flow cytometry in vitro. (4) Conclusion: After 150 days of investigation, HiFlow was considered biologically acceptable, and the formation of apatite crystal layers and the promotion of M2-like macrophage polarization may contribute to its favorable biocompatibility.
Biomechanical Evaluation of Patient-Specific Polymethylmethacrylate Cranial Implants for Virtual Surgical Planning: An In-Vitro Study
Authors: Msallem Bilal , Maintz Michaela , Halbeisen Florian S. , Meyer Simon , Sigron Guido R. , Sharma Neha , Cao Shuaishuai , Thieringer Florian M.
Published: March 2022

Cranioplasty with freehand-molded polymethylmethacrylate implants is based on decades of experience and is still frequently used in clinical practice. However, data confirming the fracture toughness and standard biomechanical tests are rare. This study aimed to determine the amount of force that could be applied to virtually planned, template-molded, patient-specific implants (n = 10) with an implant thickness of 3 mm, used in the treatment of a temporoparietal skull defect (91.87 cm2), until the implant cracks and finally breaks. Furthermore, the influence of the weight and porosity of the implant on its force resistance was investigated. The primary outcome showed that a high force was required to break the implant (mean and standard deviation 1484.6 ± 167.7 N), and this was very strongly correlated with implant weight (Pearson’s correlation coefficient 0.97; p < 0.001). Secondary outcomes were force application at the implant’s first, second, and third crack. Only a moderate correlation could be found between fracture force and the volume of porosities (Pearson’s correlation coefficient 0.59; p = 0.073). The present study demonstrates that an implant thickness of 3 mm for a temporoparietal skull defect can withstand sufficient force to protect the brain. Greater implant weight and, thus, higher material content increases thickness, resulting in more resistance. Porosities that occur during the described workflow do not seem to reduce resistance. Therefore, precise knowledge of the fracture force of polymethylmethacrylate cranial implants provides insight into brain injury prevention and serves as a reference for the virtual design process.
Three-Dimensional Printing of a Hybrid Bioceramic and Biopolymer Porous Scaffold for Promoting Bone Regeneration Potential
Authors: Hung Kuo-Sheng , Chen May-Show , Lan Wen-Chien , Cho Yung-Chieh , Saito Takashi , Huang Bai-Hung , Tsai Hsin-Yu , Hsieh Chia-Chien , Ou Keng-Liang , Lin Hung-Yang
Published: March 2022

In this study, we proposed a three-dimensional (3D) printed porous (termed as 3DPP) scaffold composed of bioceramic (beta-tricalcium phosphate (β-TCP)) and thermoreversible biopolymer (pluronic F-127 (PF127)) that may provide bone tissue ingrowth and loading support for bone defect treatment. The investigated scaffolds were printed in three different ranges of pore sizes for comparison (3DPP-1: 150–200 μm, 3DPP-2: 250–300 μm, and 3DPP-3: 300–350 μm). The material properties and biocompatibility of the 3DPP scaffolds were characterized using scanning electron microscopy, X-ray diffractometry, contact angle goniometry, compression testing, and cell viability assay. In addition, micro-computed tomography was applied to investigate bone regeneration behavior of the 3DPP scaffolds in the mini-pig model. Analytical results showed that the 3DPP scaffolds exhibited well-defined porosity, excellent microstructural interconnectivity, and acceptable wettability (θ < 90°). Among all groups, the 3DPP-1 possessed a significantly highest compressive force 273 ± 20.8 Kgf (* p < 0.05). In vitro experiment results also revealed good cell viability and cell attachment behavior in all 3DPP scaffolds. Furthermore, the 3DPP-3 scaffold showed a significantly higher percentage of bone formation volume than the 3DPP-1 scaffold at week 8 (* p < 0.05) and week 12 (* p < 0.05). Hence, the 3DPP scaffold composed of β-TCP and F-127 is a promising candidate to promote bone tissue ingrowth into the porous scaffold with decent biocompatibility. This scaffold particularly fabricated with a pore size of around 350 μm (i.e., 3DPP-3 scaffold) can provide proper loading support and promote bone regeneration in bone defects when applied in dental and orthopedic fields.
Simulation Analysis and Process Evaluation of Cooling Hole Forming Precision in Mask Assisted Electrochemical Machining Based on GH4169
Authors: Li Zhaolong , Dai Ye
Published: March 2022

Good heat dissipation performance of aero-engine an effectively improve the service performance and service life of aero-engine. Therefore, this paper studies the machining method of cooling holes of high-temperature existent material GH 4169 for aero-engine innovatively puts forward the mask electrochemical machining method of cooling holes and explores the entrance morphology and taper formation law of the hole structure of high-temperature resistant material GH 4169. The mathematical model of anode dissolution of cooling holes in ECM is established, and the influence of voltage and electrolyte flow rate on cooling holes in ECM is analyzed. Compared with the mask-less electrochemical machining, the inlet radius of cooling holes in mask electrochemical machining is reduced by about 16.0% and the taper is reduced by 52.8% under the same machining parameters, which indicates that the electrochemical machining efficiency of mask is higher and the machining accuracy is better. Experiments show that the diameter of the mask structure improves the accuracy of the inlet profile of the cooling hole in the ECM. The diameter of the mask increases from 2 mm to 2.8 mm, and the inlet radius of the cooling hole increased from 1.257 mm to 1.451 mm When the diameter of the mask is 2.2 mm, the taper of the cooling hole decreased by 53.4%. The improvement effect is best, and the thickness of the mask has little influence on the forming accuracy of the cooling hole.
Decellularized Avian Cartilage, a Promising Alternative for Human Cartilage Tissue Regeneration
Authors: Ayariga Joseph Atia , Huang Hanxiao , Dean Derrick
Published: March 2022

Articular cartilage defects, and subsequent degeneration, are prevalent and account for the poor quality of life of most elderly persons; they are also one of the main predisposing factors to osteoarthritis. Articular cartilage is an avascular tissue and, thus, has limited capacity for healing and self-repair. Damage to the articular cartilage by trauma or pathological causes is irreversible. Many approaches to repair cartilage have been attempted with some potential; however, there is no consensus on any ideal therapy. Tissue engineering holds promise as an approach to regenerate damaged cartilage. Since cell adhesion is a critical step in tissue engineering, providing a 3D microenvironment that recapitulates the cartilage tissue is vital to inducing cartilage regeneration. Decellularized materials have emerged as promising scaffolds for tissue engineering, since this procedure produces scaffolds from native tissues that possess structural and chemical natures that are mimetic of the extracellular matrix (ECM) of the native tissue. In this work, we present, for the first time, a study of decellularized scaffolds, produced from avian articular cartilage (extracted from Gallus Gallus domesticus), reseeded with human chondrocytes, and we demonstrate for the first time that human chondrocytes survived, proliferated and interacted with the scaffolds. Morphological studies of the decellularized scaffolds revealed an interconnected, porous architecture, ideal for cell growth. Mechanical characterization showed that the decellularized scaffolds registered stiffness comparable to the native cartilage tissues. Cell growth inhibition and immunocytochemical analyses showed that the decellularized scaffolds are suitable for cartilage regeneration.
Promiscuous Lipase-Catalyzed Markovnikov Addition of H-Phosphites to Vinyl Esters for the Synthesis of Cytotoxic α-Acyloxy Phosphonate Derivatives
Authors: Kowalczyk Paweł , Koszelewski Dominik , Gawdzik Barbara , Samsonowicz-Górski Jan , Kramkowski Karol , Wypych Aleksandra , Lizut Rafał , Ostaszewski Ryszard
Published: March 2022

An enzymatic route for phosphorous-carbon- bond formation is developed by discovering new promiscuous activity of lipase. This biocatalytic transformation of phosphorous-carbon- bond addition leads to biologically and pharmacologically relevant α-acyloxy phosphonates with methyl group in α-position. A series of target compounds were synthesized with yields ranging from 54% to 83% by enzymatic reaction with Candida cylindracea (CcL) lipase via Markovnikov addition of H-phosphites to vinyl esters. We carefully analyzed the best conditions for the given reaction such as the type of enzyme, temperature, and type of solvent. The developed protocol is applicable to a range of H-phosphites and vinyl esters significantly simplifying the preparation of synthetically challenging α-pivaloyloxy phosphonates. Further, the obtained compounds were validated as new potential antimicrobial drugs with characteristic E. coli bacterial strains and DNA modification recognized by the Fpg protein, N-methyl purine glycosylases as new substrates. The impact of the methyl group located in the α-position of the studied α-acyloxy phosphonates on the antimicrobial activity was demonstrated. The pivotal role of this group on inhibitory activity against selected pathogenic E. coli strains was revealed. The observed results are especially important in the case of the increasing resistance of bacteria to various drugs and antibiotics.
Bioactive Bacterial Nanocellulose Membranes Enriched with Eucalyptus globulus Labill. Leaves Aqueous Extract for Anti-Aging Skin Care Applications
Authors: Almeida Tânia , Moreira Patrícia , Sousa Fábio J. , Pereira Cláudia , Silvestre Armando J. D. , Vilela Carla , Freire Carmen S. R.
Published: March 2022

Bacterial nanocellulose (BNC) membranes, with remarkable physical and mechanical properties, emerged as a versatile biopolymeric carrier of bioactive compounds for skin care applications. In this study, BNC membranes were loaded with glycerol (as plasticizer and humectant agent) and different doses (1–3 μg cm−2) of an aqueous extract obtained from the hydro-distillation of Eucalyptus globulus Labill. leaves (HDE), for application as sheet facial masks. All membranes are resistant and highly malleable at dry and wet states, with similar or even better mechanical properties than those of a commercial BNC mask. Moreover, the HDE was found to confer a dose-dependent antioxidant activity to pure BNC. Additionally, upon 3 months of storage at 22–25 °C and 52% relative humidity (RH) or at 40 °C and 75% RH, it was confirmed that the antioxidant activity and the macroscopic aspect of the membrane with 2 μg cm−2 of HDE were maintained. Membranes were also shown to be non-cytotoxic towards HaCaT and NIH/3T3 cells, and the membrane with 2 μg cm−2 of HDE caused a significant reduction in the senescence-associated β-galactosidase activity in NIH/3T3 cells. These findings suggest the suitability and potential of the obtained membranes as bioactive facial masks for anti-aging applications.
Intermediate-Task Transfer Learning with BERT for Sarcasm Detection
Authors: Savini Edoardo , Caragea Cornelia
Published: March 2022

Sarcasm detection plays an important role in natural language processing as it can impact the performance of many applications, including sentiment analysis, opinion mining, and stance detection. Despite substantial progress on sarcasm detection, the research results are scattered across datasets and studies. In this paper, we survey the current state-of-the-art and present strong baselines for sarcasm detection based on BERT pre-trained language models. We further improve our BERT models by fine-tuning them on related intermediate tasks before fine-tuning them on our target task. Specifically, relying on the correlation between sarcasm and (implied negative) sentiment and emotions, we explore a transfer learning framework that uses sentiment classification and emotion detection as individual intermediate tasks to infuse knowledge into the target task of sarcasm detection. Experimental results on three datasets that have different characteristics show that the BERT-based models outperform many previous models.
Good Practices in Sponge Natural Product Studies: Revising Vouchers with Isomalabaricane Triterpenes
Authors: Cárdenas Paco , Gamage Jayani , Hettiarachchi Chamari M. , Gunasekera Sunithi
Published: March 2022

Species misidentification in the field of natural products is an acknowledged problem. These errors are especially widespread in sponge studies, albeit rarely assessed and documented. As a case study, we aim to revisit reports of isomalabaricane triterpenes, isolated from four demosponge genera: Jaspis, Geodia, Stelletta and Rhabdastrella. From a total of 44 articles (1981–2022), 27 unique vouchers were listed, 21 of which were accessed and re-examined here: 11 (52.4%) of these were misidentified. Overall, 65.9% of the studies published an incorrect species name: previously identified Jaspis and Stelletta species were all in fact Rhabdastrella globostellata. We conclude that isomalabaricane triterpenes were isolated from only two Rhabdastrella species and possibly one Geodia species. In addition to shedding a new light on the distribution of isomalabaricane triterpenes, this study is an opportunity to highlight the crucial importance of vouchers in natural product studies. Doing so, we discuss the impact of species misidentification and poor accessibility of vouchers in the field of sponge natural products. We advocate for stricter voucher guidelines in natural product journals and propose a common protocol of good practice, in the hope of reducing misidentifications in sponge studies, ensure reproducibility of studies, and facilitate follow-up work on the original material.
Ecological Risk Evaluation and Removal of Emerging Pollutants in Urban Wastewater by a Hollow Fiber Forward Osmosis Membrane
Authors: Salamanca Mónica , López-Serna Rebeca , Palacio Laura , Hernandez Antonio , Prádanos Pedro , Peña Mar
Published: March 2022

Forward osmosis (FO) is a promising technology for the treatment of urban wastewater. FO can produce high-quality effluents and preconcentrate urban wastewater for subsequent anaerobic treatment. This membrane technology makes it possible to eliminate the pollutants present in urban wastewater, which can cause adverse effects in the ecosystem even at low concentrations. In this study, a 0.6 m2 hollow fiber aquaporin forward osmosis membrane was used for the treatment of urban wastewater from the Valladolid wastewater treatment plant (WWTP). A total of 51 Contaminants of Emerging Concern (CECs) were investigated, of which 18 were found in the target urban wastewater. They were quantified, and their ecotoxicological risk impact was evaluated. Different salts with different concentrations were tested as draw solutions to evaluate the membrane performances when working with pretreated urban wastewater. NaCl was found to be the most appropriate salt since it leads to higher permeate fluxes and lower reverse saline fluxes. The membrane can eliminate or significantly reduce the pollutants present in the studied urban wastewater, producing water without ecotoxicological risk or essentially free of pollutants. In all cases, good recovery was achieved, which increased with molecular weight, although chemical and electrostatic interactions also played a role.
Layered Antibiofouling Composite Membrane for Quenching Bacterial Signaling
Authors: Lee Kibaek , Shah Syed Salman Ali , Park Hyeona , Lee Chung-Hak , Choo Kwang-Ho
Published: March 2022

Bacterial quorum quenching (QQ) media with various structures (e.g., bead, cylinder, hollow cylinder, and sheet), which impart biofouling mitigation in membrane bioreactors (MBRs), have been reported. However, there has been a continuous demand for membranes with QQ capability. Thus, herein, we report a novel double-layered membrane comprising an outer layer containing a QQ bacterium (BH4 strain) on the polysulfone hollow fiber membrane. The double-layered composite membrane significantly inhibits biofilm formation (i.e., the biofilm density decreases by ~58%), biopolymer accumulation (e.g., polysaccharide), and signal molecule concentration (which decreases by ~38%) on the membrane surface. The transmembrane pressure buildup to 50 kPa of the BH4-embedded membrane (17.8 h ± 1.1) is delayed by more than thrice (p < 0.05) of the control with no BH4 in the membrane’s outer layer (5.5 h ± 0.8). This finding provides new insight into fabricating antibiofouling membranes with a self-regulating property against biofilm growth.
Structure, Morphology, and Permeability of Cellulose Films
Authors: Makarov Igor S. , Golova Lyudmila K. , Bondarenko Galina N. , Anokhina Tatyana S. , Dmitrieva Evgenia S. , Levin Ivan S. , Makhatova Valentina E. , Galimova Nazym Zh. , Shambilova Gulbarshin K.
Published: March 2022

The work is focused on the study of the influence of the cellulose type and processing parameters on the structure, morphology, and permeability of cellulose films. The free volume of the cellulose films was evaluated by the sorption of n-decane, which is a non-solvent for cellulose. The structural features of the membranes and their morphology were studied using X-ray diffraction, IR spectroscopy, SEM, and AFM methods. The characteristic features of the porous structure and properties of cellulose films regenerated from cellulose solutions in the N-methylmorpholine-N-oxide (NMMO) and cellophane films were compared. Generally, cellulose films obtained from solutions in NMMO have a higher permeability and a lower rejection (as measured using Orange II dye) as compared to cellophane films. It was also found that the cellulose films have a higher ultimate strength and modulus, whereas the cellophane films are characterized by higher elongation at break.
Insight into the Structure, Functions, and Dynamics of the Leptospira Outer Membrane Proteins with the Pathogenicity
Authors: Hsu Shen-Hsing , Yang Chih-Wei
Published: March 2022

Leptospirosis is a widespread zoonosis that frequently occurs in tropical and subtropical countries. Leptospira enters the host through wounds or mucous membranes and spreads to the whole body through the blood, causing systemic infection. Kidneys are the preferential site where Leptospira accumulates, especially in the renal interstitium and renal tubule epithelial cells. Clinical symptoms in humans include high fever, jaundice, renal failure, and severe multiple-organ failure (Weil’s syndrome). Surface-exposed antigens are located at the outermost layer of Leptospira and these potential virulence factors are likely involved in primary host-pathogen interactions, adhesion, and/or invasion. Using the knockout/knockdown techniques to the evaluation of pathogenicity in the virulence factor are the most direct and effective methods and many virulence factors are evaluated including lipopolysaccharides (LPS), Leptospira lipoprotein 32 (LipL32), Leptospira ompA domain protein 22 (Loa22), LipL41, LipL71, Leptospira immunoglobulin-like repeat A (LigA), LigB, and LipL21. In this review, we will discuss the structure, functions, and dynamics of these virulence factors and the roles of these virulence factors in Leptospira pathogenicity. In addition, a protein family with special Leucine-rich repeat (LRR) will also be discussed for their vital role in Leptospira pathogenicity. Finally, these surface-exposed antigens are discussed in the application of the diagnosis target for leptospirosis and compared with the serum microscope agglutination test (MAT), the gold standard for leptospirosis.
Taurine: A Maternally Derived Nutrient Linking Mother and Offspring
Authors: Tochitani Shiro
Published: March 2022

Mammals can obtain taurine from food and synthesize it from sulfur-containing amino acids. Mammalian fetuses and infants have little ability to synthesize taurine. Therefore, they are dependent on taurine given from mothers either via the placenta or via breast milk. Many lines of evidence demonstrate that maternally derived taurine is essential for offspring development, shaping various traits in adults. Various environmental factors, including maternal obesity, preeclampsia, and undernutrition, can affect the efficacy of taurine transfer via either the placenta or breast milk. Thus, maternally derived taurine during the perinatal period can influence the offspring’s development and even determine health and disease later in life. In this review, I will discuss the biological function of taurine during development and the regulatory mechanisms of taurine transport from mother to offspring. I also refer to the possible environmental factors affecting taurine functions in mother-offspring bonding during perinatal periods. The possible functions of taurine as a determinant of gut microbiota and in the context of the Developmental Origins of Health and Disease (DOHaD) hypothesis will also be discussed.
Long Stroke Design of Piezoelectric Walking Actuator for Wafer Probe Station
Authors: Yang Cheng , Wang Yin , Fan Wei
Published: March 2022

In order to develop a high-resolution piezoelectric walking actuator with a long stroke for the wafer probe station, this work presents a design of a piezoelectric walking actuator with two auxiliary clamping feet elastically attached to major clamping feet. Its construction was introduced and its operating principle was analyzed. Structure design details were discussed and a prototype was proposed. The prototype was fabricated and tested. The experimental results show that the proposed actuator can operate stably along a 20 mm guider. The proposed design is suitable for precision motion control applications.
Considering Specific Ion Effects on Froth Stability in Sulfidic Cu-Ni-PGM Ore Flotation
Authors: Manono Malibongwe , Corin Kirsten
Published: March 2022

The mining and mineral processing of Cu-Ni-PGM sulfide ores in South Africa occurs in semi-arid regions. The scarcity of water resources in these regions has become one of the biggest challenges faced by mineral concentrators. As a result, concentrators are forced to find ways through which they can manage and control their water usage. The recycling and re-use of process water in mineral concentration plants has therefore become a common practice. This practice is beneficial in that it reduces reliance on municipal water and harnesses compliance to stringent environmental regulations on freshwater usage. This approach also offers a better response to the Sustainable Development Goals (SDGs) for the mining industry, as water and its preservation form part of the SDGs. This practice could, however, be somewhat concerning to a process operator because recirculated water often has higher concentrations of ions compared to fresh or potable water. This is because an unintended change in the process water quality may affect critical aspects of flotation such as the stability of the froth. This issue has led to the need for both the mining industry and researchers in the field to find the ions in process water that have the greatest impact on froth stability. Thus, the authors of this study investigated the effects of various ions common in the process water of a typical Cu-Ni-PGM ore on froth stability using a 3 L bench scale flotation cell. Solids and water recoveries were used as proxies for froth stability. These were further complemented by bubble size, water recoveries, foam height, and dynamic foam stability from two-phase flotation systems. A two-phase foam study resulted in observations that supported findings from a three-phase study. Generally, single salt solutions containing Ca2+ and Mg2+ ions resulted in higher water recoveries both in the two-phase foam and three-phase froth studies, increases in foam heights and dynamic foam stability, and a decrease in bubble size compared to the solutions that contained Na+. SO42− also resulted in increased foam stability compared to Cl− and NO3−. These results showed that the divalent inorganic electrolytes—Ca2+, Mg2+, and SO42−—were more froth- and foam-stabilizing than the monovalent inorganic electrolytes—Cl−, NO3−, and Na+. This finding was in agreement with previous research. The findings of this study are deemed crucial in the development of a process water management protocol in sulfidic Cu-Ni-PGM ore concentrators. However, more comparative three-phase froth stability tests are needed as subjects of future investigative work to further ascertain specific ion effects on froth stability in sulfide ores.
Model of B9N9 Response under External Electric Field: Geometry, Electronic Properties, Reaction Activity
Authors: Wu Xupu , Ren Dasen
Published: March 2022

In this paper, we performed the ωB97XD/def2-TZVP method with a density functional theory study on the boron–nitrogen (BN) analogues of cyclo[18]carbon. The geometric structure, polarization properties, and excitation effect were calculated in the presence of an external electric field (EEF). Furthermore, the dual descriptor and Fukui function matrices were employed to predict the tendency towards the electrophilic or nucleophilic reactions of B9N9 under varying EEF strengths. The results show that the application of an EEF will cause the cyclic structure of B9N9 to be considerably distorted towards an elliptical geometry, the polarization to increase, and the reactivity of B9N9 to enhance with the increase in the EEF strength. This is of great significance for further experimental exploration into the catalytic properties of BN fullerenes.
Computational Screening of Metal-Organic Frameworks for Ethylene Purification from Ethane/Ethylene/Acetylene Mixture
Authors: Zhou Yageng , Zhang Xiang , Zhou Teng , Sundmacher Kai
Published: March 2022

Identification of high-performing sorbent materials is the key step in developing energy-efficient adsorptive separation processes for ethylene production. In this work, a computational screening of metal-organic frameworks (MOFs) for the purification of ethylene from the ternary ethane/ethylene/acetylene mixture under thermodynamic equilibrium conditions is conducted. Modified evaluation metrics are proposed for an efficient description of the performance of MOFs for the ternary mixture separation. Two different separation schemes are proposed and potential MOF adsorbents are identified accordingly. Finally, the relationships between the MOF structural characteristics and its adsorption properties are discussed, which can provide valuable information for optimal MOF design.
Lemon Myrtle (Backhousia citriodora) Extract and Its Active Compound, Casuarinin, Activate Skeletal Muscle Satellite Cells In Vitro and In Vivo
Authors: Yamamoto Ayumi , Honda Shinichi , Ogura Mineko , Kato Masanori , Tanigawa Ryuichi , Fujino Hidemi , Kawamoto Seiji
Published: March 2022

Sarcopenia is an age-related skeletal muscle atrophy. Exercise is effective in improving sarcopenia via two mechanisms: activation of skeletal muscle satellite cells (SCs) and stimulation of muscle protein synthesis. In contrast, most nutritional approaches for improving sarcopenia focus mainly on muscle protein synthesis, and little is known about SC activation. Here, we investigated the effect of lemon myrtle extract (LM) on SC activation both in vitro and in vivo. Primary SCs or myoblast cell lines were treated with LM or its derived compounds, and incorporation of 5-bromo-2′-deoxyuridine, an indicator of cell cycle progression, was detected by immunocytochemistry. We found that LM significantly activated SCs (p < 0.05), but not myoblasts. We also identified casuarinin, an ellagitannin, as the active compound in LM involved in SC activation. The structure–activity relationship analysis showed that rather than the structure of each functional group of casuarinin, its overall structure is crucial for SC activation. Furthermore, SC activation by LM and casuarinin was associated with upregulation of interleukin-6 mRNA expression, which is essential for SC activation and proliferation. Finally, oral administration of LM or casuarinin to rats showed significant activation of SCs in skeletal muscle (p < 0.05), suggesting that LM and casuarinin may serve as novel nutritional interventions for improving sarcopenia through activating SCs.
Factors Associated with Overweight and Obesity in Adults from Rio Branco, Acre in the Western Brazilian Amazon
Authors: Lima Yara de Moura Magalhães , Martins Fernanda Andrade , Ramalho Alanderson Alves
Published: March 2022

This study aimed to assess factors associated with overweight and obesity in adults from Rio Branco, Acre, in the western Brazilian Amazon. This is a cross-sectional, population-based study conducted in Rio Branco, which used data on individuals aged 18 years or older collected by the 2019 National Health Survey. Software R version 4.0.5 was used to estimate the prevalence of overweight and obesity, prevalence ratios, and 95% confidence intervals. Multiple analysis was performed by Poisson’s regression with robust variance and hierarchical selection of variables. This study included 1217 adults. The prevalence of overweight was 58.2% (95%CI: 54.7–61.6) and of obesity, 20.1% (95%CI: 17.2–23.4). The factors associated with overweight were arterial hypertension (AdjPR: 1.45; 95%CI: 1.31–1.61), physical inactivity (AdjPR: 1.19; 95%CI: 1.04–1.36), age group (25–39 years, AdjPR: 1.49; 95%CI: 1.10–2.00; 40–59 years, AdjPR: 1.69; 95%CI: 1.28–2.23; 60 years or older, AdjPR: 1.37; 95%CI: 1.01–1.87); and smoking (AdjPR: 0.62; 95%CI: 0.41–0.93). The factors associated with obesity were arterial hypertension (AdjPR: 1.80; 95%CI: 1.41–2.30) and diabetes mellitus (AdjPR: 1.52; 95%CI: 1.08–2.13). Smoking and female sex remained in the hierarchical model for obesity, even without statistical significance. Despite intervention guidelines for these chronic diseases, there is a need for the public recognition of overweight and obesity and their possible associated factors in the Amazon and other regions with similar socioeconomic and demographic characteristics.
Dietary Intake of Polyphenols or Polyunsaturated Fatty Acids and Its Relationship with Metabolic and Inflammatory State in Patients with Type 2 Diabetes Mellitus
Authors: Kosmalski Marcin , Pękala-Wojciechowska Anna , Sut Agnieszka , Pietras Tadeusz , Luzak Bogusława
Published: March 2022

Background: The aim of the study was to evaluate the relationship between polyphenol or polyunsaturated fatty acids (PUFAs) consumption and the selected metabolic and inflammatory markers in type 2 diabetes (T2DM) patients. Methods: The study enrolled 129 diabetics (49 men, mean age 64.1 ± 9.8 years) with different amounts of polyphenol and PUFAs consumption. Results: A significant effect of polyphenol or PUFAs omega-3 consumption on fasting glucose concentration (FG) or glycated haemoglobin fraction (HbA1c) was reported. A negative association was observed between FG and total polyphenol, flavonoid, flavan-3-ol and stilbene intake. In the group with high flavonoid intake, the FG was significantly lower compared to the group characterised by low flavonoid intake. Polyphenols, except stilbenes, did not modulate HbA1c. Additionally, higher consumption of PUFAs omega-3 significantly decreased HbA1c, and the intake of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids negatively and significantly correlated with FG and HbA1c. Further analysis confirmed a significant association between EPA + DHA intake and HbA1c, with significant interactions with age and gender or with body mass index and waist-to-hip ratio. The dietary intake of polyphenols or PUFAs was independent of familial diabetes or diabetic diet application. Conclusions: Our study indicates a positive effect of high consumption of flavonoids, omega-3 PUFAs and stilbenes on the markers of carbohydrate metabolism balance and the absence of such an effect on other cardiometabolic markers and inflammatory conditions.
Food Addiction in Eating Disorders: A Cluster Analysis Approach and Treatment Outcome
Authors: Munguía Lucero , Gaspar-Pérez Anahí , Jiménez-Murcia Susana , Granero Roser , Sánchez Isabel , Vintró-Alcaraz Cristina , Diéguez Carlos , Gearhardt Ashley N. , Fernández-Aranda Fernando
Published: March 2022

Background: A first approach of a phenotypic characterization of food addiction (FA) found three clusters (dysfunctional, moderate and functional). Based on this previous classification, the aim of the present study is to explore treatment responses in the sample diagnosed with Eating Disorder(ED) of different FA profiles. Methods: The sample was composed of 157 ED patients with FA positive, 90 with bulimia nervosa (BN), 36 with binge eating disorder (BED), and 31 with other specified feeding or eating disorders (OSFED). Different clinical variables and outcome indicators were evaluated. Results: The clinical profile of the clusters present similar characteristics with the prior study, having the dysfunctional cluster the highest ED symptom levels, the worse psychopathology global state, and dysfunctional personality traits, while the functional one the lowest ED severity level, best psychological state, and more functional personality traits. The dysfunctional cluster was the one with lowest rates of full remission, the moderate one the higher rates of dropouts, and the functional one the highest of full remission. Conclusions: The results concerning treatment outcome were concordant with the severity of the FA clusters, being that the dysfunctional and moderate ones had worst treatment responses than the functional one.
Body Composition of Infants Born with Intrauterine Growth Restriction: A Systematic Review and Meta-Analysis
Authors: Manapurath Rukman , Gadapani Barsha , Pereira-da-Silva Luís
Published: March 2022

Intrauterine growth restriction (IUGR) may predispose metabolic diseases in later life. Changes in fat-free mass (FFM) and fat mass (FM) may explain this metabolic risk. This review studied the effect of IUGR on body composition in early infancy. Five databases and included studies from all countries published from 2000 until August 2021 were searched. Participants were IUGR or small-for-gestational age (SGA) infants, and the primary outcomes were FFM and FM. Eighteen studies met the inclusion criteria, of which seven were included in the meta-analysis of primary outcomes. Overall, intrauterine growth-restricted and SGA infants were lighter and shorter than normal intrauterine growth and appropriate-for-gestational age infants, respectively, from birth to the latest follow up. They had lower FFM [mean difference −429.19 (p = 0.02)] and FM [mean difference −282.9 (p < 0.001)]. The issue of whether lower FFM and FM as reasons for future metabolic risk in IUGR infants is intriguing which could be explored in further research with longer follow-up. This review, the first of its kind can be useful for developing nutrition targeted interventions for IUGR infants in future.
Protein Quality Changes of Vegan Day Menus with Different Plant Protein Source Compositions
Authors: Rojas Conzuelo Zaray , Bez Natalie S. , Theobald Steffen , Kopf-Bolanz Katrin A.
Published: March 2022

To underline the importance of protein quality in plant-based diets, we estimated the protein quality of different exclusively plant-protein-based day menus that are based on the “planetary health diet” developed by the EAT-Lancet Commission. PDCAAS and DIAAS were used to estimate the protein quality (PQ) and fulfilling of the amino acid recommendation for adults in vegan daily menus based on the planetary health diet: 2 days with only low-quality (LQ) protein sources and 2 days with low + high-quality (HQ) protein sources. The protein quality of Day 1LQ (DIAAS 76, PDCAAS 88) was increased by the addition of high-quality protein sources (HQPS): Day 1HQ (DIAAS 94, PDCAAS 98). Day 2LQ had a low PQ (DIAAS 71, PDCAAS 74), but when HQPS were used (Day 2HQ), the PQ increased (DIAAS 83, PDCAAS 88). Scenarios (day 1HQ, day 1LQ, and day 2 HQ) were classified as of good PQ. However, day 1LQ had a low protein quality. Consuming HQPS in a vegan diet can help to fulfil the recommendation of essential amino acids. This work served to understand and apply methods to estimate protein quality that can be applied to optimize protein mixtures to fulfil amino acid requirements in the future.