bluuebunny/crossref_metadata_2025 huggingface dataset:
Interventions and Strategies to Improve Sexual and Reproductive Health Outcomes among Adolescents Living in Low- and Middle-Income Countries: A Systematic Review and Meta-Analysis
Authors: Meherali Salima , Rehmani Mehnaz , Ali Sonam , Lassi Zohra S.
Published: September 2021

Adolescent access to quality sexual and reproductive health and rights has been a major issue in most low- to middle-income countries (LMICs). This systematic review aims to identify the relevant community and school-based interventions that can be implemented in LMICs to promote adolescents’ sexual and reproductive health and rights. We identified 54 studies, and our review findings suggested that educational interventions, financial incentives, and comprehensive post-abortion family planning services were effective in increasing their knowledge and use of Adolescent Sexual and Reproductive Health and Rights (ASRHR) services, such as contraception, which led to a decrease in unwanted pregnancies. However, we found inconclusive and limited evidence on the effectiveness of interventions for improved violence prevention and adolescent behavior towards safe sexual practices. More rigorous studies with long-term follow-ups are needed to assess the effectiveness of such interventions.
Prediction of Aircraft Noise Impact with Application to Hong Kong International Airport
Authors: Wu Chunhui , Redonnet Stephane
Published: September 2021

As part of a collective research effort towards greener aviation, the present study focuses on the noise impact of aircraft operations around major airports. To this end, an aircraft noise prediction platform is developed, which relies on state-of-the-art functionalities as well as more specific, innovative features. Originally built upon the Aircraft Noise and Performance (ANP) database and its Noise–Power–Distance (NPD) table, the method is further refined to alleviate most of their inherent limitations (e.g., standardized and simplified aircraft noise scenarios). The resulting aircraft noise prediction platform is validated against benchmark cases of increasing complexity, being then applied to real-life situations involving actual aircraft operations around Hong Kong International Airport (HKIA). Specific comparative analyses are conducted, which allow highlighting the variability of the noise impact by aircraft, depending on their type (A330, B777) and/or operational conditions (power settings, meteorological conditions, routes, banks, etc.). The study delivers insightful outcomes, whether phenomenological (aircraft noise impact) or methodological (aircraft noise prediction). As a by-product, it illustrates how noise prediction methods/platforms such as the present one may help in guiding the further expansion of airport operations and/or infrastructures (as is currently the case with HKIA).
Induced Agricultural Production Organizations under the Transition of Rural Land Market: Evidence from China
Authors: Fang Da , Guo Yan
Published: September 2021

This paper focuses on the impact of a new rural land reform, the Separation of Three Rights Reform, on changes in China’s agricultural production organizations. We illustrate the impact of market and nonmarket mechanisms on allocating agricultural production factors under the new rural land market transition through a land system and factor allocation model. Based on the expansion paths of different types of factors in the model, we classify the development of Chinese agricultural production entities into “extensional expansion,” “labor-intensive expansion,” “land-intensive expansion,” and “exit of agricultural production.” These agricultural production paths correspond to agricultural enterprises, family farms, agricultural cooperatives, and small farmers’ exit. Further, empirical and economic geography analysis results show that the interaction of market and nonmarket mechanisms is the main drive that induces the current diversified organizations in rural China. Thus, this paper provides a comprehensive explanation of changing patterns of an agricultural production organization under the transition of the rural land market.
Exploring the Governance and Fairness in the Milk Value Chain: A Case Study in Vietnam
Authors: Hoang Viet , Nguyen An , Hubbard Carmen , Nguyen Khanh-Duy
Published: September 2021

Governance and fairness in the food value chain have gained considerable attention from both policymakers and scholars, especially in developing countries. This study analysed the milk value chain, exploring its governance and fairness, and assessed the regulatory interventions across the milk value chain in Vietnam using a qualitative framework and the global value chain governance model. The results show that Vietnam’s milk production and dairy market have developed notably since the reforms. The value chain is structured according to three governance models, i.e., relational, captive, and hierarchy models. Vietnam’s milk value chain has progressed through three building phases, expanding in breadth, and undergoing in-depth development, and the governance models have adjusted accordingly. However, Vietnamese dairy farms have been exposed to a low level of fairness across the supply chain. Although dairy farmers in the relational model may benefit from more power and fairness in the short term, farmers in the captive model may gain benefits and potential fairness in the long term. Vietnam has diverse regulatory interventions to enhance farmers’ fairness and welfare, and the results are notable. However, not all farmers have benefitted from these policies, and measures regarding fairness and welfare should be diverse, gradual, and inclusive.
Preliminary Study of Slaughter Value and Meat Characteristics of 18 Months Ostrich Reared in Hungary
Authors: Brassó Lili Dóra , Szabó Vanessza , Komlósi István , Pusztahelyi Tünde , Várszegi Zsófia
Published: September 2021

The study aimed to evaluate the slaughter value and meat characteristics of ten ostrich females reared and slaughtered at the age of 18 months in Hungary. The ratio of selected body parts, the main organs and the lean meat parts were examined. The nutritive composition, the colour, the technological and organoleptic characteristics of five valuable meat parts (outside strip—M. flexor cruris lateralis, oyster—M. iliofemoralis externus, tip—M. femorotibialis medius, outside leg—M. gastrocnemius pars externa, medal—M. ambiens) and the amino acid, fatty acid and mineral composition of outside strip (M. flexor cruris lateralis) were also evaluated. The ratio of body parts and the main organs as the percentage of live weight, and the lean meat part as the percentage of carcass weight showed 16.74 ± 0.01%, 6.16 ± 0.01% and 57.29 ± 0.59%, respectively. The dry matter content of the examined valuable meat parts ranged between 24.89 ± 0.08 and 26.23 ± 0.13%, the protein ratio took on values between 18.40 ± 0.09 and 20.62 ± 0.16%, the fat content showed values between 2.36 ± 0.07 and 4.50 ± 1.09% and the hydroxyproline content ranged between 0.01 ± 0.001 and 0.08 ± 0.001%. The amino acid content of the outside strip showed a range between 0.15 and 3.33%. The ratio of SFA, MUFA and PUFA was 35.10 ± 0.53, 37.37 ± 1.52 and 27.54 ± 1.01. The n-6/n-3 ratio showed 3.91 ± 0.43 and the SFA/UFA ratio was 0.54 ± 0.02. Among the examined minerals, the content of Ca, K, Mg, Na and P was the highest in the meat. In the case of the colour, regarding L* value, we could reveal no significant difference between the examined meat parts. For a* and b* values, the outside leg had the lowest data of all. We could not reveal a significant difference between the pH values of the meat parts. Regarding technological parameters, meat differed only in thawing loss. The significantly lowest thawing loss could be detected in the outside leg (2.72 ± 0.01%) and in the medal (2.32 ± 0.01%). The results of the organoleptic evaluation showed that the outside strip and the tip had the best flavour and tenderness. In comparison with the younger birds (10–14 months of age) in the literature, the 18-month-old ostriches in our study showed similar or slightly lower slaughter weight, skin weight and head ratio, greater liver weight, lighter meat, lower protein and higher fat content, higher essential amino acid and lower non-essential amino acid content and higher SFA content in some cases. However, data on nutrition and population size were not always available. In comparison with other ratites (emu and rhea), ostrich meat has lower dry matter and protein, but higher fat, SFA, MUFA and PUFA content and lower n-6/n-3 ratio.
Erratum: Mulesa et al. Pluralistic Seed System Development: A Path to Seed Security? Agronomy 2021, 11, 372
Authors:   Agronomy Editorial Office
Published: September 2021

The authors would like to make the following correction to the published paper [...]
Morphological and Ecogeographic Study of the Diversity of Cassava (Manihot esculenta Crantz) in Ecuador
Authors: Monteros-Altamirano Álvaro , Tapia Cesar , Paredes Nelly , Alulema Valeria , Tacán Marcelo , Roura Alberto , Lima Luis , Sørensen Marten
Published: September 2021

Cassava (Manihot esculenta Crantz) is a crop of nutritional and economic importance worldwide, cultivated in more than 100 tropical and subtropical countries including Ecuador, where it is traditionally cultivated in the three continental regions: Amazonia, the Coast and in the valleys of the Sierra. The purpose of this study is to characterize 195 accessions from INIAP’s Ecuadorian cassava collection through (1) morphological characterization with qualitative and quantitative descriptors; and (2) ecogeographic characterization to know the climatic, geophysical, and edaphic conditions in which cassava grows and which environments are frequent or marginal for its cultivation. For the morphological characterization, 27 morphological descriptors were used (18 qualitative and nine quantitative), and for the ecogeographic characterization, 55 variables (41 climatic, two geophysical and 12 edaphic). Four morphological groups and three ecogeographic groups were identified. Morphological variability was evidenced mainly in descriptors related to the leaves, stems, and inflorescences. In addition, it was possible to identify accessions that appear capable of growing under extreme conditions of drought and poor soils. These accessions could be used for improvement.
Intensive Production and Management of Marandu Palisadegrass (Urochloa brizantha ‘Marandu’) Accelerates Leaf Turnover but Does Not Change Herbage Mass
Authors: Ongaratto Fernando , Fernandes Marcia Helena Machado da Rocha , Dallantonia Erick Escobar , Lima Lais de Oliveira , Val Guilherme Alves do , Cardoso Abmael da Silva , Rigobello Izabela Larosa , Campos Jorge Augusto Americo , Reis Ricardo Andrade , Ruggieri Ana Claudia , Malheiros Euclides Braga
Published: September 2021

Pasture intensification through higher stocking rates, nitrogen fertilization and intensified grazing management in beef cattle production optimizes pasture use by increasing the forage harvested. We aimed to assess its effects on the morphogenesis and canopy structure of Urochloa brizantha ‘Marandu’ (marandu palisadegrass) pastures. The treatments consisted of marandu palisadegrass pastures managed under continuous stocking and a canopy height of 25 cm, with different levels of intensification: extensive, semi-intensive, and intensive systems N-fertilized with 0 kg, 75 kg, and 150 kg N ha−1 year−1, respectively, as ammonium nitrate (32% of N), with four replicates (paddocks) in a completely randomized design. Phyllochron (9.8 days) and leaf lifespan (34.7 days) were shorter in intensified pastures, whereas herbage mass was similar among treatments. Extensive pastures had a higher proportion of senescent material; thus, more intensive systems showed higher proportions of leaves and stems, although the leaves-to-stem ratio remained similar across production systems. The defoliation interval was lower in intensive (14.4 days) and higher in extensive (18.7 days) treatments. Thus, pasture intensification accelerates leaf appearance, decreases leaf lifespan, shortens the tiller defoliation interval and increased herbage accumulation rate but does not change herbage mass. The extensive system produces excessive forage losses due to dead material.
Ion-Exclusion/Cation-Exchange Chromatography Using Dual-Ion-Exchange Groups for Simultaneous Determination of Inorganic Ionic Nutrients in Fertilizer Solution Samples for the Management of Hydroponic Culture
Authors: Kozaki Daisuke , Sago Yuki , Fujiwara Taku , Mori Masanobu , Kubono Chihiro , Koga Tougo , Mitsui Yuta , Tachibana Tomotaka
Published: September 2021

In this study, ion-exclusion/cation-exchange chromatography (IEC/CEC) using dual-ion-exchange groups (carboxy and sulfo groups) for the simultaneous determination of anions (SO42−, Cl−, NO3−, and HPO42−) and cations (Na+, NH4+, K+, Mg2+, and Ca2+) was developed. By using the combination of dual-ion-exchange groups, simultaneous separation of inorganic ions with HPO42− was achieved that was impossible by the conventional IEC/CEC based on the single-ion-exchange group (carboxy group). This method was applied to the monitoring of inorganic ionic nutrients in fertilizer solution samples in hydroponic culture. As a result, a higher peak resolution of inorganic anions and cations with phosphate ion using IEC/CEC with dual-ion-exchange groups was achieved in the absence of matrix effects. In addition, the developed method helps to understand the behavior of ionic nutrients in fertilizer solution during hydroponic cultivation and is potentially useful for the individual fertilization of ionic nutrients.
Behavioural and Physiological Changes in a Herd of Arabian Mares after the Separation of Individuals Differently Ranked within the Dominance Hierarchy
Authors: Stachurska Anna , Wiśniewska Anna , Kędzierski Witold , Różańska-Boczula Monika , Janczarek Iwona
Published: September 2021

Horses in a herd develop and maintain a dominance hierarchy between all individuals. There are many situations in riding facilities and studs in which horses have to be separated out of a group. The aim of the study was to determine the rate of behaviours, level of locomotor activity and cardiac activity variables in a herd of horses during a short social separation of individuals differently ranked in the dominance hierarchy. Twelve adult Arabian mares were involved. A behavioural test had been performed before the main experiment to determine the rank order of the mares in this social herd. Three tests were performed when a dominant, mixed and submissive three-member group of mares was separated for 10 min. The response of the remaining herd was determined by a rate of behaviours, time of locomotor activity and cardiac parameters. The results of the experiment reveal evident changes towards emotional arousal in the social herd elicited by a short separation of some conspecifics. The herd created by humans preserves the sensitivity to a temporary loss of its members. The response of the remaining herd does not depend strictly on the composition of the separated mares regarding their rank in the dominance hierarchy.
Validation of On-Farm Bacteriological Systems for Endometritis Diagnosis in Postpartum Dairy Cows
Authors: Barbeau-Grégoire Nicolas , Boyer Alexandre , Rousseau Marjolaine , Gauthier Marie-Lou , Dubuc Jocelyn
Published: September 2021

The objective of this study was to validate the accuracy of the results of on-farm bacteriological culture media (Tri-plate and Petrifilm) from endometrial samples compared with the ones from the diagnostic laboratory. A cross-sectional observational study was set up within two dairy herd clients of the Université de Montréal. A total of 189 cows in the postpartum period were systematically enrolled to collect two uterine samples from cytobrushes during the same examination. The first cytobrush was used to inoculate the Tri-plate medium directly and then was sent to the reference laboratory for aerobic bacterial culture. The second cytobrush was used to make a microscopic smear for cytological analysis (proportion of polymorphonuclear cells) and subsequently diluted in 1 mL of saline to inoculate the Petrifilm medium. From these data, statistical analyses were computed to optimize the summation of sensitivity and specificity of the two systems compared with the results of the reference laboratory. For the Tri-plate and Petrifilm media, the cutoffs of ˃90 and ˃100 colonies gave the maximum sum of sensitivity and specificity, respectively. In conclusion, Tri-plate media was best at reproducing the results obtained by laboratory analysis using a threshold of >90 colonies.
Detection and Visualization of Heterozygosity-Rich Regions and Runs of Homozygosity in Worldwide Sheep Populations
Authors: Selli Alana , Ventura Ricardo V. , Fonseca Pablo A. S. , Buzanskas Marcos E. , Andrietta Lucas T. , Balieiro Júlio C. C. , Brito Luiz F.
Published: September 2021

In this study, we chose 17 worldwide sheep populations of eight breeds, which were intensively selected for different purposes (meat, milk, or wool), or locally-adapted breeds, in order to identify and characterize factors impacting the detection of runs of homozygosity (ROH) and heterozygosity-rich regions (HRRs) in sheep. We also applied a business intelligence (BI) tool to integrate and visualize outputs from complementary analyses. We observed a prevalence of short ROH, and a clear distinction between the ROH profiles across populations. The visualizations showed a fragmentation of medium and long ROH segments. Furthermore, we tested different scenarios for the detection of HRR and evaluated the impact of the detection parameters used. Our findings suggest that HRRs are small and frequent in the sheep genome; however, further studies with higher density SNP chips and different detection methods are suggested for future research. We also defined ROH and HRR islands and identified common regions across the populations, where genes related to a variety of traits were reported, such as body size, muscle development, and brain functions. These results indicate that such regions are associated with many traits, and thus were under selective pressure in sheep breeds raised for different purposes. Interestingly, many candidate genes detected within the HRR islands were associated with brain integrity. We also observed a strong association of high linkage disequilibrium pattern with ROH compared with HRR, despite the fact that many regions in linkage disequilibrium were not located in ROH regions.
The Impact of the Adjunct Heat-Treated Starter Culture and Lb. helveticus LH-B01 on the Proteolysis and ACE Inhibitory Activity in Dutch-Type Cheese Model during Ripening
Authors: Garbowska Monika , Berthold-Pluta Anna , Stasiak-Różańska Lidia , Pluta Antoni
Published: September 2021

Adjunct cultures are used in cheesemaking to improve the sensory characteristics of the ripened cheeses. In addition, it is known that different adjunct cultures are capable of producing enzymes with the specificity to hydrolyze caseins, leading to the release of various bioactive compounds (bioactive peptides, amino acids, etc.). The objective of this study was to evaluate the effect of adjunct heat-treated starter XT–312 and a cheese culture Lb. helveticus LH-B01 on the proteolytic activity and angiotensin converting enzymes inhibitors (ACE) in cheese models during ripening. Seven different cheese models were evaluated for: proteolytic activity using the spectrophotometric method with ortho-phthaldialdehyde (OPA), soluble nitrogen (SN), trichloroacetic acid-soluble nitrogen (TCA-SN) phosphotungstic acid-soluble nitrogen (PTA-SN), total nitrogen (TN), pH, contents of water, fat, as well as for total bacteria count (TBC), count of Lactococcus genus bacteria, count of Lb. helveticus, and number of non-starter lactic acid bacteria (NSLAB). Presence of adjunct bacterial cultures both in the form of a cheese culture LH-B01 and heat-treated XT–312 starter promoted primary and secondary proteolysis, which resulted in acceleration of the ripening process. ACE inhibitory activity and proteolytic activity was the highest throughout of ripening for cheese model with LH-B01 culture. The cheese models with the adjunct heat-treated starter were characterized by lower TBC, NSLAB and lower count of Lactococcus genus bacteria during ripening, compared to control cheeses.
Prevalence and Characteristics of Staphylococcus aureus Associated with Meat and Meat Products in African Countries: A Review
Authors: Thwala Thembeka , Madoroba Evelyn , Basson Albert , Butaye Patrick
Published: September 2021

Antimicrobial resistance has been increasing globally, which negatively affects food safety, veterinary, and human medicine. Ineffective antibiotics may cause treatment failure, which results in prolonged hospitalisation, increased mortality, and consequently, increased health care costs. Staphylococcus aureus causes a diverse range of infections including septicaemia and endocarditis. However, in food, it mainly causes food poisoning by the production of enterotoxins. With the discovery of methicillin-resistant S. aureus strains that have a separate reservoir in livestock animals, which were termed as livestock-associated methicillin-resistant S. aureus (LA-MRSA) in 2005, it became clear that animals may pose another health risk. Though LA-MRSA is mainly transferred by direct contact, food transmission cannot be excluded. While the current strains are not very pathogenic, mitigation is advisable, as they may acquire new virulence genes, becoming more pathogenic, and may transfer their resistance genes. Control of LA-MRSA poses significant problems, and only Norway has an active mitigation strategy. There is limited information about LA-MRSA, MRSA in general, and other S. aureus infections from African countries. In this review, we discuss the prevalence and characteristics of antimicrobial susceptible and resistant S. aureus (with a focus on MRSA) from meat and meat products in African countries and compare it to the situation in the rest of the world.
New Insight on Antibiotic Resistance and Virulence of Escherichia coli from Municipal and Animal Wastewater
Authors: Gregova Gabriela , Kmet Vladimir , Szaboova Tatiana
Published: September 2021

Antibiotic resistance of the indicator microorganism Escherichia coli was investigated in isolates from samples collected during the course of one year from two wastewater treatment plants treating municipal and animal wastes in Slovakia, respectively. The genes of antibiotic resistance and virulence factors in selected resistant E. coli isolates were described. A high percentage of the isolates from municipal and animal wastewater were resistant to ampicillin, streptomycin, tetracycline, ceftiofur, ceftriaxone, and enrofloxacin. In the selected E. coli isolates, we detected the following phenotypes: ESBL (20.4% in animal wastewater; 7.7% in municipal wastewater), multidrug-resistant (17% of animal and 32% of municipal isolates), high resistance to quinolones (25% of animal and 48% of municipal samples), and CTX-M (7.9% of animal and 17.3% of municipal isolates). We confirmed an integro-mediated antibiotic resistance in 13 E. coli strains from municipal and animal wastewater samples, of which the Tn3 gene and virulence genes cvaC, iutA, iss, ibeA, kps, and papC were detected in six isolates. One of the strains of pathogenic E. coli from the animal wastewater contained genes ibeA with papC, iss, kpsII, Int1, Tn3, and Cit. In addition, one blaIMP gene was found in the municipal wastewater sample. This emphasises the importance of using the appropriate treatment methods to reduce the counts of antibiotic-resistant microorganisms in wastewater effluent.
Concomitant Treatment with Voriconazole and Flucloxacillin: A Combination to Avoid
Authors: Van Daele Ruth , Wauters Joost , De Cock Pieter , Buyle Franky , Leys John , Van Brantegem Pieter , Gijsen Matthias , Annaert Pieter , Debaveye Yves , Lagrou Katrien , Peetermans Willy E. , Brüggemann Roger J. , Spriet Isabel
Published: September 2021

Background: Voriconazole is an antifungal drug used as one of the first-line treatments for invasive aspergillosis. This drug is extensively metabolized, predominantly via cytochrome P450 enzymes. An interaction between flucloxacillin and voriconazole, leading to subtherapeutic voriconazole concentrations, has previously been reported. We aimed to demonstrate that flucloxacillin independently influences voriconazole exposure. Methods: Patients from three Belgian hospitals, treated with a combination of voriconazole and flucloxacillin, were included in this retrospective study. Voriconazole concentrations were collected both in a timeframe with and without flucloxacillin co-treatment. Multivariate analyses were performed to study the independent effect of flucloxacillin treatment on voriconazole exposure and the possible influence of the flucloxacillin dose. Results: Thirty-three patients were included in this study and 145 trough concentrations (51 with, and 94 without concomitant flucloxacillin treatment) were analyzed. The median (IQR) voriconazole trough concentration sampled during flucloxacillin co-treatment was 0.5 (0–1.8) mg/L, whereas samples without flucloxacillin co-treatment had a median (IQR) voriconazole trough concentration of 3.5 (1.7–5.1) mg/L (p = 0.002), while receiving similar voriconazole doses. Subtherapeutic concentrations (<1 mg/L) were observed in 69% and 7% of the samples with flucloxacillin co-treatment versus samples without flucloxacillin co-treatment, respectively. Conclusion: This study shows that flucloxacillin co-treatment independently decreases voriconazole exposure. Caution is needed when these two drugs are administered simultaneously.
Empowering Patients to Self-Manage Common Infections: Qualitative Study Informing the Development of an Evidence-Based Patient Information Leaflet
Authors: Hayes Catherine V. , Mahon Bláthnaid , Sides Eirwen , Allison Rosie , Lecky Donna M. , McNulty Cliodna A. M.
Published: September 2021

Common self-limiting infections can be self-managed by patients, potentially reducing consultations and unnecessary antibiotic use. This qualitative study informed by the Theoretical Domains Framework (TDF) aimed to explore healthcare professionals’ (HCPs) and patients’ needs on provision of self-care and safety-netting advice for common infections. Twenty-seven patients and seven HCPs participated in semi-structured focus groups (FGs) and interviews. An information leaflet was iteratively developed and reviewed by participants in interviews and FGs, and an additional 5 HCPs, and 25 patients (identifying from minority ethnic groups) via online questionnaires. Qualitative data were analysed thematically, double-coded, and mapped to the TDF. Participants required information on symptom duration, safety netting, self-care, and antibiotics. Patients felt confident to self-care and were averse to consulting with HCPs unnecessarily but struggled to assess symptom severity. Patients reported seeking help for children or elderly dependents earlier. HCPs’ concerns included patients’ attitudes and a lack of available monitoring of advice given to patients. Participants believed community pharmacy should be the first place that patients seek advice on common infections. The patient information leaflet on common infections should be used in primary care and community pharmacy to support patients to self-manage symptoms and determine when further help is required.
L-Methionine Protects against Oxidative Stress and Mitochondrial Dysfunction in an In Vitro Model of Parkinson’s Disease
Authors: Catanesi Mariano , Brandolini Laura , d’Angelo Michele , Benedetti Elisabetta , Tupone Maria Grazia , Alfonsetti Margherita , Cabri Enrico , Iaconis Daniela , Fratelli Maddalena , Cimini Annamaria , Castelli Vanessa , Allegretti Marcello
Published: September 2021

Methionine is an aliphatic, sulfur-containing, essential amino acid that has been demonstrated to have crucial roles in metabolism, innate immunity, and activation of endogenous antioxidant enzymes, including methionine sulfoxide reductase A/B and the biosynthesis of glutathione to counteract oxidative stress. Still, methionine restriction avoids altered methionine/transmethylation metabolism, thus reducing DNA damage and possibly avoiding neurodegenerative processes. In this study, we wanted to study the preventive effects of methionine in counteracting 6-hydroxydopamine (6-OHDA)-induced injury. In particular, we analyzed the protective effects of the amino acid L-methionine in an in vitro model of Parkinson’s disease and dissected the underlying mechanisms compared to the known antioxidant taurine to gain insights into the potential of methionine treatment in slowing the progression of the disease by maintaining mitochondrial functionality. In addition, to ascribe the effects of methionine on mitochondria and oxidative stress, methionine sulfoxide was used in place of methionine. The data obtained suggested that an L-methionine-enriched diet could be beneficial during aging to protect neurons from oxidative imbalance and mitochondrial dysfunction, thus preventing the progression of neurodegenerative processes.
Signaling Overlap between the Golgi Stress Response and Cysteine Metabolism in Huntington’s Disease
Authors: Paul Bindu D.
Published: September 2021

Huntington’s disease (HD) is caused by expansion of polyglutamine repeats in the protein huntingtin, which affects the corpus striatum of the brain. The polyglutamine repeats in mutant huntingtin cause its aggregation and elicit toxicity by affecting several cellular processes, which include dysregulated organellar stress responses. The Golgi apparatus not only plays key roles in the transport, processing, and targeting of proteins, but also functions as a sensor of stress, signaling through the Golgi stress response. Unlike the endoplasmic reticulum (ER) stress response, the Golgi stress response is relatively unexplored. This review focuses on the molecular mechanisms underlying the Golgi stress response and its intersection with cysteine metabolism in HD.
Optimised Extraction of Archaeological Features from Full 3-D GPR Data
Authors: Forte Emanuele , Mocnik Arianna , Basso Patrizia , Casagrande Giulia , Martinucci Davide , Pillon Simone , Possamai Marco , Zambrini Roberta
Published: September 2021

The use of non-invasive methodologies is becoming essential for archaeological research, and ground penetrating radar is one of the most important techniques to obtain high resolution information. In this paper we present the analysis of a full 3-D GPR dataset integrated with a high-resolution photogrammetric survey acquired in a Roman archaeological site located in Aquileia (Northeast Italy) within the partially excavated area known as “Fondo Pasqualis”. We evaluated the importance of dense and accurate data collection and of processing of the GPR signal for characterization of the archaeological features. We further discuss the parametrization and the applicability of GPR attributes, in particular amplitude-based and coherence attributes, to better identify and characterise the archaeological buried targets. Furthermore, autopicking procedures for isosurfaces mapping were critically evaluated with the objective of detecting complex structures. The final interpretation of all the GPR features, with the support of digital terrain modelling and orthophotos from unmanned aerial vehicles, guided the archaeologists to open and excavate newly selected areas, which revealed interesting structures and contributed to the understanding of the historical events that characterized the Aquileia city.
Neuroimaging of Pediatric Cerebellum in Inherited Neurodegenerative Diseases
Authors: Chiapparini Luisa , Moscatelli Marco
Published: September 2021

In the study of cerebellar degenerative diseases, morphologic imaging (computed tomography, CT and magnetic resonance imaging, MRI) is the most common examination. From the clinical and genetic point of view, cerebellar degenerative diseases include heterogeneous conditions in which MRI may show isolated cerebellar atrophy or cerebellar atrophy associated with other cerebellar or supratentorial abnormalities. Neuroradiological progression is often observed. In congenital disorders of glycosylation (CDG), for example, MRI may be normal, may demonstrate mild cerebellar atrophy or, in the advanced stages of the disease, marked atrophy of the cerebellar hemispheres and vermis associated with the abnormal signal intensity of the cerebellar cortex and white matter and brainstem hypotrophy. In spinal cerebellar ataxias (SCAs), very rare in the pediatric population, MRI may demonstrate isolated cerebellar atrophy or cerebellar and brainstem atrophy. MRI shows characteristic findings in other diseases, strongly suggesting a distinct disorder, such as neuroaxonal dystrophy, ARSACS, ataxia-telangiectasia, or precise mitochondrial diseases. An example of neurodegenerative disorder with prenatal onset is pontocerebellar hypoplasia (PCH). PCH represents a group of neurodegenerative disorders characterized by microcephaly, early cerebellar hypoplasia, and variable atrophy of the cerebellum and ventral pons, genetically divided into several subtypes. Cerebellar hypoplasia visible on MRI is often the first sign that suggests the clinical diagnosis. In most cases, the PCH subtype may demonstrate a characteristic pattern distinguishable at MRI. Selective involvement of the cerebellum, sometimes accompanied by brainstem or supratentorial abnormalities in different combinations, may help restrict the differential diagnosis and may address the specific molecular screening.
Nonlinear Effect and MAI Impact on SAC-OCDMA System Based on 2D Multi-Diagonal Code and Laser Array
Authors: Mrabet Hichem , Bahloul Faouzi , Karar Abdullah , Cherifi Abdelhamid , Belghith Aymen
Published: September 2021

A new architecture for Spectral Amplitude Coding Optical Code Division Multiple Access (SAC-OCDMA) system based on two Dimensional Multi Diagonal (2D-MD) codes named 2D-MD SAC-OCDMA and utilizing a laser optical source is proposed for Long-Reach Passive Optical Network (LR-PON). In this work, a computer simulator tool is used, for the first time, as a SAC-OCDMA simulation set-up utilizing the unique combination of a coherent laser array and 2D-MD codes. In addition, the system performance is addressed numerically by taking into account Multiple Access Interference (MAI), optical coherent source noise, first, second and third order fiber dispersion, nonlinear effects and photo-detector noise. Simulation results indicate that for a single user (i.e., without considering MAI), the system can operate at a maximum bit rate of 55 Gb/s over 250 km of Single Mode Fiber (SMF), with a Bit Error Rate (BER) below 10−9 (Q-limit = 15.5 dB), when only first order fiber dispersion is considered. However, including the effects of second and third order fiber dispersion as frequency domain parameters, results in a reduction of the maximum bit rate to 40 Gb/s, while maintaining a Q-factor above the Q-limit under the same transmission distance. Furthermore, we demonstrate that the proposed architecture extends the SMF transmission reach up to 600 km and 480 km, when considering linear and nonlinear effects, respectively. Finally, we show that our proposed 2D-MD SAC-OCDMA system outperforms existing solutions presented in the literature for LR-PON configuration, in terms of both aggregate bit rate and transmission reach.
High Vacuum Packaging of MEMS Devices Containing Heterogeneous Discrete Components
Authors: Guo Ping , Meng Hongling , Dan Lin , Xu Hao , Zhao Jianye
Published: September 2021

Vacuum packaging of Micro-electro-mechanical system (MEMS) devices is a hot topic for its advantages of improving performance and reducing power consumption. In this paper, the physics package of a chip scale atomic clock (CSAC), as a typical kind of MEMS device, is performed by vacuum packaging based on a systematic method proposed by us. The whole process, including low outgassing and thermal stable materials selection, prebaking for desorption, getter firing for absorption and solder reflow for vacuum sealing is introduced thoroughly. The thermogravimetric analysis or thermal gravimetric analysis (TGA) is used to analyze the thermal stability and desorption of materials. The leak rate of physics packages is measured to be less than 4 × 10−10 Pa·m3/s by helium leak detection. The residual gas pressure and composition in physics packages are analyzed after vacuum packaging. The results show a high vacuum ~0.1 Pa in the physics package. The frequency stability is improved from 4.68 × 10−11 to 1.07 × 10−11 @40,000 s. The presented method for high vacuum packaging is also applicable to other MEMS devices.
Collagen-Based Bioactive Bromelain Hydrolysate from Salt-Cured Cod Skin
Authors: Coscueta Ezequiel R. , Brassesco María Emilia , Pintado Manuela
Published: September 2021

Considerable amounts of fish processing by-products are discarded each year. About 30% of this material may be skin and bone. Fish skin has more than 80% of its total protein content as collagen. Furthermore, in recent years, there has been a growing demand for collagen-based peptides due to their beneficial health effects. So, the objective of the present study was to optimise the obtaining bioactive hydrolysates from salt-cured cod skin using the protease Bromelain at 0.5% (w/w) concentration. This study developed a sustainable process that consumes less time and energy and uses an alternative source as raw material. In addition, bromelain allows hydrolysates with important antioxidant (ORAC, 514 μmol Trolox Equivalent/g protein) and antihypertensive activities (inhibition of ACE, IC50 of 166 μg protein/mL) as well as excellent biocompatibility with dermal and subcutaneous cells.
Sustained Loading Bond Response and Post-Sustained Loading Behaviour of NSM CFRP-Concrete Elements under Different Service Temperatures
Authors: Gómez Javier , Barris Cristina , Baena Marta , Perera Ricardo , Torres Lluís
Published: September 2021

Nowadays, one of the foremost procedures for strengthening concrete structures is the Near-Surface Mounted (NSM) technique. This paper presents an experimental study on the effect sustained loading and different service temperatures (steady and cyclic) have on NSM Carbon Fibre-Reinforced Polymer (CFRP)-concrete bonded joints and their post-sustained loading load-slip behaviour. Four experimental campaigns using eight NSM CFRP-concrete specimens were performed by employing two different service load levels (15% and 30% of the ultimate load) and combining two groove thicknesses (7.5 and 10 mm) and two bonded lengths (150 and 225 mm). Two steady state temperatures (20 and 40 °C) and two cyclic service temperatures (ranging between 20 and 40 °C) were programmed. The slip obtained was proportional to the sustained load level. Furthermore, higher slips were registered for specimens under higher mean temperatures in the cycle. After 1000 h of sustained load testing, the specimens were tested under monotonic loading until failure (post-sustained loading tests). In general, the ratio between the post-sustained loading ultimate load and the instantaneous ultimate load was close to the unity, although some differences were perceived in series S2 (steady 37.7 °C) with a mean increase of 6.3%, and series S3-B (cyclic temperature ranging between 24.6 and 39.2 °C) with a mean reduction of 9%.
Property Analysis of Photo-Polymerization-Type 3D-Printed Structures Based on Multi-Composite Materials
Authors: Hwang So-Ree , Park Min-Soo
Published: September 2021

Additive manufacturing, commonly called 3D printing, has been studied extensively because it can be used to fabricate complex structures; however, polymer-based 3D printing has limitations in terms of implementing certain functionalities, so it is limited in the production of conceptual prototypes. As such, polymer-based composites and multi-material 3D printing are being studied as alternatives. In this study, a DLP 3D printer capable of printing multiple composite materials was fabricated using a movable separator and structures with various properties were fabricated by selectively printing two composite materials. After the specimen was fabricated based on the ASTM, the basic mechanical properties of the structure were compared through a 3-point bending test and a ball rebound test. Through this, it was shown that structures with various mechanical properties can be fabricated using the proposed movable-separator-based DLP process. In addition, it was shown that this process can be used to fabricate anisotropic structures, whose properties vary depending on the direction of the force applied to the structure. By fabricating multi-joint grippers with varying levels of flexibility, it was shown that the proposed process can be applied in the fabrication of soft robots as well.
Design and Experimental Analyses of Hybrid Piston Rods Used in Hydraulic Cylinders under Axial Load
Authors: S P Praveen Kumar , Lee Seok-Soon
Published: September 2021

Composite hydraulic cylinders are used to reduce the weight of construction equipment such as aerial work platforms or excavators. The weight is compensated by manufacturing hydraulic cylinders from Carbon Fiber Reinsforced Plastic (CFRP), but this is expensive. Therefore, this study investigated a hybrid hydraulic cylinder, which is a combination of CFRP and steel, considering both performance and cost. The conventional hydraulic cylinder rods are made of steel, which can prevent failure due to buckling under push load (Push) or failure under alternating push–pull load (Push–Pull) or pull only. In this paper, we discuss how the failure threshold for these two mechanisms can be increased by making the piston rod from a hybrid material. In order to develop this lightweight hybrid piston rod for hydraulic cylinders that meets the buckling strength requirements of the original steel rod, CFRP is used as a substitute, which has significant buckling strength against compressive loading and, most importantly, is lighter than steel. The substitution is done either by replacing steel completely with CFRP or by reducing the volume of steel and sheathing it with CFRP. Numerical and experimental studies are carried out to understand the strength and behavior of piston rods when they are replaced by different combinations of composite materials for the given load. For this study, two different piston rod designs with various design parameters were considered, and their respective behavior under loading was discussed. The effect of compressive loads on CFRP wrapped steel parts and buckling strength as a function of fiber orientation, stacking angle and number of CFRP layers was investigated using experiments. The study demonstrated the usefulness of steel-CFRP composites to reduce weight and their influence on buckling load in hydraulic cylinders.
Resective Surgery versus Debridement in Stage 2 Medication-Related Osteonecrosis of the Jaw
Authors: D’Amato Salvatore , Troiano Antonio , Lo Giudice Giorgio , De Cicco Davide , Rusciano Mario , Tartaro Gianpaolo , Colella Giuseppe
Published: September 2021

The aim of this study is to analyze surgical and functional outcomes in order to verify the applicability of surgical treatment guidelines as foreseen by MASCC/ISOO/ASCO 2019. Patients affected by stage 2 MRONJ refractory to conservative management were grouped if underwent surgical resection (Group A) or debridement (Group B). Health-related quality of life was evaluated by using the European Organization for Research and Treatment of Cancer questionnaires, QLQ-C30 and H&N35. Statistical analysis was performed using Wilcoxon/Mann–Whitney test, Kaplan–Meier test, Cox regression model and Cox multivariate regression. Group A showed higher complete healing cases vs. group B. Recurrence rate difference in group A vs. group B was statistically significant. Debridement is an unfavorable prognostic factor when compared to surgical resection (p = 0.0032, HR 4.9). Quality of life mean values showed a marked improvement in Group A and a slight improvement in Group B. Debridement has 4.9 times more risk to develop recurrence when compared to surgical resection. A more satisfactory quality of life was shown in patients subject to resective surgery with an improving trend from baseline. Debridement patients showed more variability of results and an overall negative trend at the end of the 6-month follow-up.
Review of Materials and Fabrication Methods for Flexible Nano and Micro-Scale Physical and Chemical Property Sensors
Authors: Nyabadza Anesu , Vázquez Mercedes , Coyle Shirley , Fitzpatrick Brian , Brabazon Dermot
Published: September 2021

The use of flexible sensors has tripled over the last decade due to the increased demand in various fields including health monitoring, food packaging, electronic skins and soft robotics. Flexible sensors have the ability to be bent and stretched during use and can still maintain their electrical and mechanical properties. This gives them an advantage over rigid sensors that lose their sensitivity when subject to bending. Advancements in 3D printing have enabled the development of tailored flexible sensors. Various additive manufacturing methods are being used to develop these sensors including inkjet printing, aerosol jet printing, fused deposition modelling, direct ink writing, selective laser melting and others. Hydrogels have gained much attention in the literature due to their self-healing and shape transforming. Self-healing enables the sensor to recover from damages such as cracks and cuts incurred during use, and this enables the sensor to have a longer operating life and stability. Various polymers are used as substrates on which the sensing material is placed. Polymers including polydimethylsiloxane, Poly(N-isopropylacrylamide) and polyvinyl acetate are extensively used in flexible sensors. The most widely used nanomaterials in flexible sensors are carbon and silver due to their excellent electrical properties. This review gives an overview of various types of flexible sensors (including temperature, pressure and chemical sensors), paying particular attention to the application areas and the corresponding characteristics/properties of interest required for such. Current advances/trends in the field including 3D printing, novel nanomaterials and responsive polymers, and self-healable sensors and wearables will also be discussed in more detail.
An Alternative Conceptual Model for the Spent Nuclear Fuel–Water Interaction in Deep Geologic Disposal Conditions
Authors: Pastina Barbara , LaVerne Jay A.
Published: September 2021

For the long-term safety assessment of direct disposal of spent nuclear fuel in deep geologic repositories, knowledge on the radionuclide release rate from the UO2 matrix is essential. This work provides a conceptual model to explain the results of leaching experiments involving used nuclear fuel or simulant materials in confirmed reducing conditions. Key elements of this model are: direct effect of radiation from radiolytic species (including defects and excited states) in the solid and in the first water layers in contact with its surface; and excess H2 may be produced due to processes occurring at the surface of the spent fuel and in confined water volumes, which may also play a role in keeping the spent fuel surface in a reduced state. The implication is that the fractional radionuclide release rate used in most long-term safety assessments (10−7 year−1) is over estimated because it assumes that there is net UO2 oxidation caused by radiolysis, in contrast with the alternative conceptual model presented here. Furthermore, conventional water radiolysis models and radiation chemical yields published in the literature are not directly applicable to a heterogeneous system such as the spent fuel–water interface. Suggestions are provided for future work to develop more reliable models for the long-term safety assessment of spent nuclear fuel disposal.