bluuebunny/crossref_metadata_2025 huggingface dataset:
Generosity's double‐edged sword: Unmasking the impact of raised social assistance rates in Norway
Authors: Lorentzen Thomas , Dahl Espen
Published: March 2024

As long as welfare arrangements have been in existence, there has been a strong belief that high‐benefit generosity leads to welfare reliance. In this study, we investigate whether an increase in welfare generosity in Norway resulted in higher social assistance (SA) uptake and decreased engagement in paid work. By utilizing high‐quality administrative data and employing a difference‐in‐difference design, we find no overall effects on SA or work activity. However, we do observe a significant reduction in work activity and an increase in SA for specific predefined high‐risk groups, which are believed to be particularly responsive to financial incentives. Thus, we discover evidence of unfavorable effects for child families, non‐Western immigrants, and the combined group of non‐Western immigrant child families. These latter findings are interpreted in light of the particular socioeconomic circumstances of these groups.
Preparation of (Ti0.2Zr0.2Hf0.2Nb0.2Ta0.2)C high‐entropy ceramic nanopowders via liquid‐phase precursor
Authors: Xie Chenyi , Miao Huaming , Wan Fan , Wang Yanfei , Li Duan , Liu Rongjun
Published: March 2024

Using citric acid, ethylene glycol, ZrOCl2·8H2O, HfOCl2·8H2O, TiCl4, NbCl5, and TaCl5 as raw materials, the (Ti0.2Zr0.2Hf0.2Nb0.2Ta0.2)C high‐entropy ceramic precursor was prepared by Pechini coordination polymerization method. The precursor and pyrolysis products were analyzed and characterized by different methods. The results showed that the precursor formed a chelate structure, which greatly improved the stability of the precursor, and was clear and transparent with moderate viscosity (30–50 mPa·s). At a low pyrolysis temperature of 1600°C, single‐phase (Ti0.2Zr0.2Hf0.2Nb0.2Ta0.2)C high‐entropy ceramic nanopowders with a ceramic yield of 50.8% can be obtained. The high‐entropy ceramic powder has high purity, a particle size of about 140–200 nm, and uniform element distribution. It is suitable for introduction into fiber‐reinforced ceramic matrix composite by precursor infiltration and pyrolysis (PIP) process.
Clinical supervision experience of nurses in or transitioning to advanced practice: A systematic review
Authors: Zonneveld Debbie , Conroy Tiffany , Lines Lauren
Published: March 2024

Aims

To systematically explore the clinical supervision (CS) experience for nurses transitioning to advanced practice.

Design

A qualitative systematic review using Joanna Briggs Institute meta‐aggregation following an a priori protocol published on PROSPERO (CRD42023426658).

Data Sources

Qualitative studies obtained from Medline, CINAHL, PsycINFO, Scopus, Emcare and ERIC databases and ProQuest dissertations and theses for peer‐reviewed, published and unpublished studies from inception to July 2023.

Review Methods

Two authors conducted data screening and abstraction. Quality was appraised using the Critical Appraisal Skills Programme tool and reporting followed the Enhancing Transparency in Reporting the Synthesis of Qualitative Research checklist for systematic reviews.

Results

Sixteen studies contributed to five synthesized findings: CS that is beneficial requires structure and commitment, trusting relationships are foundational for learning, lifting burdens and preventing burnout, learning through reflection, critical thinking and feedback and barriers to CS.

Conclusions

This review provides a meaningful exploration of CS to support nurses transitioning to advanced practice. Well‐structured supervision offers a safe space to share work‐related concerns and develop an advanced practitioner identity. Sharing experiences helps alleviate work‐related burdens and reduce professional isolation and burnout.

Implications for the Profession

Peer‐support networks are vital for successful transition to advanced practice.

Impact

This review highlighted the impact of effective supervisory relationships in forming professional identity and possible links with nursing retention.

Public Contribution

No direct patient contributions are included as it forms part of a research degree.
Nonclinical evaluation of chronic cardiac contractility modulation on 3D human engineered cardiac tissues
Authors: Feaster Tromondae K. , Ewoldt Jourdan K. , Avila Anna , Casciola Maura , Narkar Akshay , Chen Christopher S. , Blinova Ksenia
Published: March 2024

Introduction

Cardiac contractility modulation (CCM) is a medical device‐based therapy delivering non‐excitatory electrical stimulations to the heart to enhance cardiac function in heart failure (HF) patients. The lack of human in vitro tools to assess CCM hinders our understanding of CCM mechanisms of action. Here, we introduce a novel chronic (i.e., 2‐day) in vitro CCM assay to evaluate the effects of CCM in a human 3D microphysiological system consisting of engineered cardiac tissues (ECTs).

Methods

Cryopreserved human induced pluripotent stem cell‐derived cardiomyocytes were used to generate 3D ECTs. The ECTs were cultured, incorporating human primary ventricular cardiac fibroblasts and a fibrin‐based gel. Electrical stimulation was applied using two separate pulse generators for the CCM group and control group. Contractile properties and intracellular calcium were measured, and a cardiac gene quantitative PCR screen was conducted.

Results

Chronic CCM increased contraction amplitude and duration, enhanced intracellular calcium transient amplitude, and altered gene expression related to HF (i.e., natriuretic peptide B, NPPB) and excitation‐contraction coupling (i.e., sodium‐calcium exchanger, SLC8).

Conclusion

These data represent the first study of chronic CCM in a 3D ECT model, providing a nonclinical tool to assess the effects of cardiac electrophysiology medical device signals complementing in vivo animal studies. The methodology established a standardized 3D ECT‐based in vitro testbed for chronic CCM, allowing evaluation of physiological and molecular effects on human cardiac tissues.
Perspective on Temporomandibular Joint Disorder: Foramen Tympanicum Defect
Authors: Ozmen Elif Esra , Satir Samed , Icen Veysel
Published: March 2024

Background

Foramen tympanicum (FT) is a defect located anterior‐inferior to the external acoustic meatus. We evaluated its prevalence, location, size, and relationship with temporomandibular joint (TMJ) disorders.

Methods

Cone beam computed tomography was performed for 200 patients who presented to the Karamanoğlu Mehmetbey University Ahmet Keleşoğlu Faculty of Dentistry Hospital. The location and size of the FT in the axial and sagittal planes were evaluated. Descriptive statistics were used to compare the study parameters among age and sex groups. Patients with FT were reevaluated by two maxillofacial surgeons at the study centre.

Results

In total, 200 images from 400 joints were examined. Unilateral and bilateral FT (19 [9.5%] and 8 [4%], respectively) was detected in 35 (17.5%) images from 27 (13.5%) patients. Examinations were performed for TMJ disorders in 24 patients. Participants with bilateral defects had the highest rates of presence of sounds and ear pain on the left and right sides (p < .05).

Conclusion

Foramen tympanicum can lead to TMJ disorders and spread of tumours or infections from the external auditory canal to the infratemporal fossa. The increased prevalence of such disorders in patients with bilateral FT suggests an association between them.
Evaluation of taurine and carnitine concentrations in whole blood, plasma, skeletal muscle and cardiac muscle in dogs
Authors: McCauley Sydney R. , Clark Stephanie D. , Leach Stacey B. , Quest Bradley W. , Streeter Renee M.
Published: March 2024

Little is known about how plasma and whole blood taurine and plasma carnitine correlate to concentrations in skeletal and cardiac muscle and the effects of diet in dogs. The purpose of this study was to evaluate the correlation among plasma, skeletal and cardiac muscle carnitine and taurine and whole blood taurine and determine the effect of diet. The study protocol was approved by the Pet Food Solutions Institutional Animal Care and Use Committee. Thirty‐three mixed‐breed hounds and 32 beagles were evaluated at Day 0 then removed from their baseline diet and randomized to a test diet: high animal protein, grain‐inclusive (HA‐GI), low animal protein, grain‐free (LA‐GF), low animal protein, grain‐inclusive (LA‐GI), or high animal protein, grain‐free (HA‐GF). Blood was drawn every 30 days and endomyocardial (mixed breeds only) and skeletal muscle biopsies were collected at Days 0 and 180. The correlations between plasma and whole blood taurine, or plasma carnitine and skeletal and cardiac muscle concentrations were weak (p < 0.01–0.05). Mixed‐breed hounds had increased (p = 0.029) whole blood taurine compared to beagles. Plasma taurine was lower with diet HA‐GF, (p = 0.009) however, all diets had increased taurine from Day 0 and were, on average within the laboratory reference range. Dogs fed the HA‐GI diet had increased cardiac muscle carnitine esters (p = 0.014). Increased carnitine esters were also appreciated in cardiac muscle in all diets from Day 0 to 180 (p = 0.0001). On Day 180 mixed‐breed hounds had increased skeletal total carnitine (p < 0.001) compared to all time points and breeds. This study observed no correlation between plasma, whole blood, skeletal and cardiac muscle taurine concentrations but noted some effects between time, breed and diet.
Retrospective evaluation of leflunomide as an adjunctive therapy in dogs with non‐associative immune‐mediated thrombocytopenia: 20 cases (2008‐2021)
Authors: Spear D. J. , Crouse Z. J. , Kearns S. A.
Published: March 2024

Objective

To describe leflunomide as an adjunctive therapy in the treatment of non‐associative immune‐mediated thrombocytopenia.

Materials and Methods

A retrospective study of dogs with a diagnosis of non‐associative immune‐mediated thrombocytopenia treated with leflunomide March 2008 to September 2021 was conducted. Data collected included signalment, clinical signs, physical examination findings and diagnostic testing performed. Medications administered, duration of hospital stay, time to platelet concentration >150×109/L and adverse events during leflunomide therapy were recorded. Relapses within a year of diagnosis were reported.

Results

A total of 20 client‐owned dogs met inclusion criteria. Nineteen of 20 dogs (95%) achieved a platelet concentration >150×109/L with leflunomide and prednisone combination therapy and four dogs (21.1%) relapsed during treatment or shortly after treatment. Adverse effects included diarrhoea (n=5), mild lymphopenia (n=9) and mild intermittent anaemia (n=1). A single dog developed hepatotoxicity presumed to be secondary to leflunomide therapy that resolved after drug discontinuation. One dog was treated for aspiration pneumonia during treatment. Two dogs were euthanased while receiving leflunomide.

Clinical Significance

Length of hospitalisation, time to platelet recovery, treatment response and relapse rate were comparable with alternative treatment protocols. Most adverse effects did not require leflunomide dose adjustment; however, two dogs died while undergoing leflunomide treatment and there is compelling evidence that one of these dogs experienced fatal infection secondary to immune‐suppression. Hepatotoxicity remains a known complication of leflunomide treatment and serial biochemistry testing is recommended.
Standards of proficiency for registered nurses—To what end? A critical analysis of contemporary mental health nursing within the United Kingdom context
Authors: Bifarin Oladayo , Collier‐Sewell Freya , Smith Grahame , Moriarty Jo , Shephard Han , Andrews Lauren , Pearson Sam , Kasperska Mari
Published: March 2024

Against the backdrop of cultural and political ideals, this article highlights both the significance of mental health nursing in meeting population needs and the regulatory barriers that may be impeding its ability to adequately do so. Specifically, we consider how ambiguous notions of ‘proficiency’ in nurse education—prescribed by the regulator—impact the development of future mental health nurses and their mental health nursing identity. A key tension in mental health practice is the ethical‐legal challenges posed by sanctioned powers to restrict patients' freedom at the same time as the desire (and obligation) to promote patients' self‐determined recovery. The genericism of the UK's Future Nurse Standards do little to prepare mental health nurses to navigate the tensions that ensue. This has consequences for nurses and patients alike, as both risk experiencing the distress and dissonance that attends giving or receiving poor care. We argue that more needs to be done to enable mental health nurses to define and articulate the nuances of the profession as part of becoming critical, thoughtful and confident practitioners. Educators can contribute to this mission by aligning curriculum, pedagogy and assessment to create meaningful opportunities for mental health nursing students to engage with the complexities of mental health nursing practice. Without this, the credibility of the profession will continue to be questioned; its future uncertain.
Long‐term shift towards shady and nutrient‐rich habitats in Central European temperate forests
Authors: Vild Ondřej , Chudomelová Markéta , Macek Martin , Kopecký Martin , Prach Jindřich , Petřík Petr , Halas Petr , Juříček Michal , Smyčková Marie , Šebesta Jan , Vojík Martin , Hédl Radim
Published: March 2024

Biodiversity world‐wide has been under increasing anthropogenic pressure in the past century. The long‐term response of biotic communities has been tackled primarily by focusing on species richness, community composition and functionality. Equally important are shifts between entire communities and habitat types, which remain an unexplored level of biodiversity change.

We have resurveyed > 2000 vegetation plots in temperate forests in central Europe to capture changes over an average of five decades. The plots were assigned to eight broad forest habitat types using an algorithmic classification system. We analysed transitions between the habitat types and interpreted the trend in terms of changes in environmental conditions.

We identified a directional shift along the combined gradients of canopy openness and soil nutrients. Nutrient‐poor open‐canopy forest habitats have declined strongly in favour of fertile closed‐canopy habitats. However, the shift was not uniform across the whole gradients.

We conclude that the shifts in habitat types represent a century‐long successional trend with significant consequences for forest biodiversity. Open forest habitats should be urgently targeted for plant diversity restoration through the implementation of active management. The approach presented here can be applied to other habitat types and at different spatio‐temporal scales.
Survival does not differ by annual center transplant volume—A Pediatric Heart Transplant Society Registry study
Authors: Ybarra A. Marion , Kamsheh Alicia M. , O'Connor Matthew J. , Hollander Seth A. , Bano Maria , Ploutz Michelle , Vaughn Gabrielle , Lambert Andrea , Wallendorf Michael , Kirklin James , Canter Charles E.
Published: March 2024

Background

There are conflicting data regarding the relationship between center volume and outcomes in pediatric heart transplantation. Previous studies have not fully accounted for differences in case mix, particularly in high‐risk congenital heart disease (CHD) groups. We aimed to evaluate the relationship between center volume and outcomes using the Pediatric Heart Transplant Society (PHTS) Registry and explore how case mix may affect outcomes.

Methods

A retrospective cohort study of all pediatric patients in the PHTS Registry who received a heart transplant from 2009 to 2018 was performed. Centers were divided into 5 groups based on average yearly transplant volume. The primary outcome was time to death or graft loss and outcomes were compared using Kaplan–Meier analysis.

Results

There were 4583 cases among 55 centers included. There was no difference in time to death or graft loss by center volume in the entire cohort (p = .75), in patients with CHD (p = .79) or in patients with cardiomyopathy (p = .23). There was also no difference in time to death or graft loss by center size in patients undergoing transplant after Norwood, Glenn or Fontan (log rank p = .17, p = .31, and p = .10 respectively). There was a statistically significant difference in outcomes by center size in the positive crossmatch group (p < .0001), though no discernible pattern related to high or low center volume.

Conclusions

Outcomes are similar among transplant centers of all sizes, including for high‐risk patient groups with CHD. Future work is needed to understand how patient‐specific risk factors may vary among centers of various sizes and whether this influences patient outcomes.
Liver transplant for primary biliary tract neuroendocrine tumor in a nine‐year‐old girl
Authors: Rai Anjali , Sproule Lauren , Larman Tatianna , Oshima Kiyoko , Rhee Daniel , Ng Kenneth , King Elizabeth , Mogul Douglas , Lemberg Kathryn
Published: March 2024

Background

Neuroendocrine tumors (NETs) are rare epithelial neoplasms that arise most commonly from the gastrointestinal tract. In pediatrics, the most common site of origin is in the appendix, with the liver being the most common site of metastasis. Neuroendocrine tumors arising from the biliary tract are extremely rare.

Methods

We describe a case of a nine‐year‐old girl who presented with obstructive cholestasis and was found to have multiple liver masses identified on biopsy as well‐differentiated neuroendocrine tumor with an unknown primary tumor site.

Result

The patient underwent extensive investigation to identify a primary tumor site, including endoscopy, endoscopic ultrasound, and capsule endoscopy. The patient ultimately underwent definitive management with liver transplant, and on explant was discovered to have multiple well‐differentiated neuroendocrine tumors, WHO Grade 1, with extensive infiltration into the submucosa of bile duct, consistent with primary biliary tract neuroendocrine tumor.

Conclusion

Identifying the site of the primary tumor in NETs found within the liver can be challenging. To determine if an extrahepatic primary tumor exists, workup should include endoscopy, EUS, and capsule endoscopy. Children with well‐differentiated hepatic NETs, with no identifiable primary tumor, and an unresectable tumor, are considered favorable candidates for liver transplantation.
Evaluation of a commercial proAKAP4 kit for the assessment of fresh and frozen–thawed feline spermatozoa
Authors: Prochowska Sylwia , Eberhardt Maria , Niżański Wojciech
Published: March 2024

ProAKAP4 is gaining increasing attention as a potential marker of semen quality in many species, but while there is a commercial kit for assessing proAKAP4 in the domestic cat, there are no publications about its use. The aim of this study was to evaluate the commercial proAKAP4 kit – Cat 4MID® Kit (SPQI – 4BioDx, Lille, France) for the assessment of feline semen. Semen was collected from 54 male cats by urethral catheterization. After a basic semen evaluation (subjective motility, CASA, viability, morphology), proAKAP4 levels in each sample were assessed using the Cat 4MID® Kit according to the manufacturer's protocol or with some modifications related to incubation time, sample storage conditions and number of spermatozoa used. Finally, the Spearman correlation of proAKAP4 concentration and sperm motility parameters was calculated. The most reliable results (acceptable intraassay coefficient of variation) were obtained with an optimized protocol of overnight incubation and isolation of proAKAP4 protein from 1 × 106 spermatozoa stored at −80°C. For fresh semen, there were no significant correlations between proAKAP4 concentration and sperm motility parameters, despite a strong correlation between motility parameters and sperm viability and morphology. A predominant effect of other sperm parameters and highly variable performance of lysis buffer question the usefulness of Cat 4MID® Kit for the assessment of feline semen. For frozen–thawed semen, there was a moderate, negative correlation between proAKAP4 concentration and two CASA parameters, VAP and VSL. As there were no correlations between proAKAP4 concentration in fresh semen and motility parameters in cryopreserved samples, proAKAP4 cannot be used as freezability marker in cats. More studies are needed to establish potential correlation with long‐lasting motility.
Impact of surgical lung biopsy on lung function and survival in patients with idiopathic pulmonary fibrosis in a multi‐centre registry cohort
Authors: Marcoux Veronica , Lok Stacey D. , Mondal Prosanta , Assayag Deborah , Fisher Jolene H. , Shapera Shane , Morisset Julie , Manganas Hélène , Fell Charlene D. , Hambly Nathan , Cox P. Gerard , Kolb Martin , Gershon Andrea S. , To Teresa , Sadatsafavi Mohsen , Khalil Nasreen , Wong Alyson W. , Wilcox Pearce G. , Ryerson Christopher J. , Vu Thao , Johannson Kerri A.
Published: March 2024

Background and Objective

Establishing an accurate and timely diagnosis of idiopathic pulmonary fibrosis (IPF) is essential for appropriate management and prognostication. In some cases, surgical lung biopsy (SLB) is performed but carries non‐negligible risk. The objective of this retrospective study was to determine if SLB is associated with accelerated lung function decline in patients with IPF using the Canadian Registry for Pulmonary Fibrosis.

Methods

Linear mixed models and Cox proportional hazards regression models were used to compare decline in forced vital capacity (FVC)%, diffusion capacity of the lung (DLCO%) and risk of death or lung transplantation between SLB and non‐SLB patients. Adjustments were made for baseline age, sex, smoking history, antifibrotic use, and lung function. A similar analysis compared lung function changes 12 months pre‐ and post‐SLB.

Results

A total of 81 SLB patients and 468 non‐SLB patients were included. In the SLB group, the post‐biopsy annual FVC% decline was 2.0% (±0.8) in unadjusted, and 2.1% (±0.8) in adjusted models. There was no difference in FVC% decline, DLCO% decline, or time to death or lung transplantation between the two groups, in adjusted or unadjusted models (all p‐values >0.07). In the pre‐post SLB group, no differences were identified in FVC% decline in unadjusted or adjusted models (p = 0.07 for both).

Conclusion

No association between SLB and lung function decline or risk of death or lung transplantation was identified in this multi‐centre study of patients with IPF.

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Detection of the HLA‐B*40:01:35 allele in a Taiwanese individual
Authors: Yang Kuo‐Liang , Lin Py‐Yu
Published: March 2024

Nucleotide substitutions in codons −1 and 84 of HLA‐B*40:01:01 result in a novel allele, HLA‐B*40:01:35.
The Prospect of the GCC Monetary Union and its Expansion to Other MENA Countries
Authors: Kamara Mohamed Sheriff Hamid
Published: March 2024

This study investigated the possibility of expanding the GCC monetary union to other MENA countries using cluster analysis for the period 2000-2018. It also examined whether the economic characteristics of the countries provide evidence for the readiness of countries for the proposed union. The findings show that there was heterogeneity in the GCC and MENA countries suggesting that the countries were not yet economically ready for the launch of the monetary union. We found evidence in support of the expansion of the GCC monetary union to other MENA countries with two categories of countries, the core and the periphery. We also revealed key variables, including inflation differential, debt service requirement, and current account balance, which clearly demarcated the GCC and other MENA countries. These findings underscore the need for policy-makers in MENA countries to strengthen intraregional trade creating policy, policy coordination, and harmonisation for a successful and sustainable monetary union.
SoK: Analyzing Privacy and Security of Healthcare Data from the User Perspective
Authors: Tazi Faiza , Nandakumar Archana , Dykstra Josiah , Rajivan Prashanth , Das Sanchari
Published: March 2024

Interactions in healthcare, by necessity, involve sharing sensitive information to achieve high-quality patient outcomes. Therefore, sensitive data must be carefully protected. This article explores existing privacy and security research conducted in the context of healthcare organizations. We conducted a systematic literature review of
N
=1,553 articles that examine the security and privacy of healthcare data and focus on 80 articles addressing human factors. Key findings show that much of the healthcare security and privacy research is focused on technology (44.11%, 712 articles), with a lack of emphasis on the human element (4.96%, 80 articles). In the subset of user studies, we find that patients and the general public express concerns about privacy and security with technologies like electronic health records (EHRs). Furthermore, our analysis shows that healthcare professionals often have low awareness of risks related to data security. Additionally, our analysis revealed that most research focuses narrowly on large hospitals, neglecting private practices and the unique challenges they face. We conclude by identifying research gaps and providing potential solutions to enable robust data security for sensitive patient data.
Nascent shifts in renal cellular metabolism, structure, and function due to chronic empagliflozin in prediabetic mice
Authors: Shepard Blythe D. , Chau Jennifer , Kurtz Ryan , Rosenberg Avi Z. , Sarder Pinaki , Border Samuel P. , Ginley Brandon , Rodriguez Olga , Albanese Chris , Knoer Grace , Greene Aarenee , De Souza Aline M. A. , Ranjit Suman , Levi Moshe , Ecelbarger Carolyn M.
Published: March 2024

Renal protection afforded by sodium glucose transporter, type 2 inhibitors (SGLT2i), e.g., empagliflozin (EMPA) involves complex intertwined mechanisms. Using a novel mouse model of obesity with insulin resistance, the TallyHo/Jng (TH) mouse on a high-milk-fat diet (HMFD), we found subtle changes in metabolism including altered regulation of sodium transporters that line the renal tubule. New potential epigenetic determinants of metabolic changes relating to FOXO and cancer signaling pathways were elucidated from an altered urine exosomal microRNA signature.
Extracellular glucose and dysfunctional insulin receptor signaling independently upregulate arterial smooth muscle TMEM16A expression
Authors: Raghavan Somasundaram , Brishti Masuma Akter , Bernardelli Angelica , Mata-Daboin Alejandro , Jaggar Jonathan H. , Leo M. Dennis
Published: March 2024

The calcium-activated chloride channel, TMEM16A, is upregulated in the diabetic vasculature to cause increased vasoconstriction. In this paper, we have uncovered that the TMEM16A gene expression is controlled by a Snail-mediated repressor complex that uncouples with both insulin-dependent and -independent pathways to allow for upregulated arterial protein expression thereby causing vasoconstriction. The paper highlights the effect of short- and long-term glucose-induced dysfunction of an ion channel expression as a causative factor in diabetic vascular disease.
Massive Osteolysis and Pseudotumor Formation following Maestro Total Wrist Arthroplasty
Authors: Sagerfors Marcus , Reiser Daniel
Published: March 2024

Metallosis is a known complication of arthroplasty and has been reported for the hip, knee, and shoulder joints. Metallosis pseudotumors have been linked to an increased risk of implant failure. We report a case of pseudotumor with massive bone loss following total wrist arthroplasty (TWA) using the Maestro implant. Revision to arthrodesis is possible, but issues with bone loss have to be addressed. We recommend caution in offering TWA to young patients with high functional demands.
Orthopantomography Detection of Atheroma Plaques and Its Relationship with Periodontal Disease and Missing Teeth
Authors: Quevedo García Rodrigo , Arnaiz Díez Sara , Pérez Pevida Esteban , Del Río Solá María Lourdes
Published: March 2024

Background. The aim of this study is to determine the atheromatous plaques’ prevalence in orthopantomography and their relationship with periodontal disease and missing teeth. Material and Methods. Orthopantomographs of 1,254 patients over 18 years of age from Clínica Arlanza in Lerma, Burgos, were examined between 2017 and 2021. A Planmeca ProOne® orthopantomograph (68 kV, 7 mA, and 10 sg) was used. Statistical analysis was carried out using SPSS Statistics® version 25. The results of the categorical variables were described as frequencies (%). Contingency tables were made with the qualitative variables, and the chi-square test was applied to study the relationship among them. The measure of statistical power used was the relative risk (RR), which was described with its respective 95% confidence interval (CI). Student’s t-test was applied to study the relationship between the qualitative variable “presence or absence of atheroma plaque” and the quantitative variable “number of teeth.” Results. A 6.2% prevalence of atheroma plaques was obtained from 1,079 selected X-rays. The risk in patients with periodontal disease increased as periodontal disease worsened. The risk in patients with periodontal disease increased as periodontal disease worsened as follows: healthy patients vs. periodontal patients with less than 30% bone loss in radiography: RR 0.434, 95% CI 0.181–1.041, p=0.053 healthy patients vs. patients with between 30%–60% bone loss: RR 0.177, 95% CI 0.075–0.418, p<0.05 healthy patients vs. patients with more than 60% bone loss: RR 0.121, 95% CI 0.041–0.355, p<0.05. Patients with calcifications on their orthopantomograms had a lower mean teeth number (20.9 teeth) compared to patients without calcifications (24 teeth), which was statistically significant, t (1077) = −3.125, p<0.05. Conclusions. Orthopantomography can be considered a screening method to detect patients at increased cardiovascular risk who are referred for individualized study. It is important to continue research to know the real significance of these findings. Dentists should be aware of the importance of our work in our patients’ systemic health.
Supplementary File 1 from Adipose Triglyceride Lipase Is a Therapeutic Target in Advanced Prostate Cancer That Promotes Metabolic Plasticity
Authors: Awad Dominik , Cao Pham Hong Anh , Pulliam Thomas L. , Spradlin Meredith , Subramani Elavarasan , Tellman Tristen V. , Ribeiro Caroline F. , Muzzioli Riccardo , Jewell Brittany E. , Pakula Hubert , Ackroyd Jeffrey J. , Murray Mollianne M. , Han Jenny J. , Leng Mei , Jain Antrix , Piyarathna Badrajee , Liu Jingjing , Song Xingzhi , Zhang Jianhua , Klekers Albert R. , Drake Justin M. , Ittmann Michael M. , Coarfa Cristian , Piwnica-Worms David , Farach-Carson Mary C. , Loda Massimo , Eberlin Livia S. , Frigo Daniel E.
Published: March 2024

Supplementary Dataset of Phosphoproteomics Analyses of Patient Samples
Table S5 from The Epigenetic Evolution of Glioma Is Determined by the IDH1 Mutation Status and Treatment Regimen
Authors: Malta Tathiane M. , Sabedot Thais S. , Morosini Natalia S. , Datta Indrani , Garofano Luciano , Vallentgoed Wies , Varn Frederick S. , Aldape Kenneth , D'Angelo Fulvio , Bakas Spyridon , Barnholtz-Sloan Jill S. , Gan Hui K. , Hasanain Mohammad , Hau Ann-Christin , Johnson Kevin C. , Cazacu Simona , deCarvalho Ana C. , Khasraw Mustafa , Kocakavuk Emre , Kouwenhoven Mathilde C.M. , Migliozzi Simona , Niclou Simone P. , Niers Johanna M. , Ormond D. Ryan , Paek Sun Ha , Reifenberger Guido , Sillevis Smitt Peter A. , Smits Marion , Stead Lucy F. , van den Bent Martin J. , Van Meir Erwin G. , Walenkamp Annemiek , Weiss Tobias , Weller Michael , Westerman Bart A. , Ylstra Bauke , Wesseling Pieter , Lasorella Anna , French Pim J. , Poisson Laila M. , Woehrer Adelheid , Lowman Allison K , deCarvalho Ana C , Castro Ana Valeria , Transou Andrea , Brodbelt Andrew R , Hau Ann-Christin , Lasorella Anna , Golebiewska Anna , Walenkamp Annemiek , Molinaro Annette M , Iavarone Antonio , Ismail Azzam , Westerman Bart A , Ylstra Bauke , Bock Christoph , Ormond D. Ryan , Brat Daniel J , Kocakavuk Emre , Van Meir Erwin G , Barthel Floris P , Varn Frederick S , D'Angelo Fulvio , Finocchiaro Gaetano , Rao Ganesh , Zadeh Gelareh , Reifenberger Guido , ngNg Ho Keu , Kim Hoon , Noushmehr Houtan , Miletic Hrvoje , Gan Hui K , Datta Indrani , Rock Jack , Snyder James M , Huse Jason T , Connelly Jennifer M , Barnholtz-Sloan Jill S , Niers Johanna M , deGroot John F , Akdemir Kadir C , Kannan Kasthuri S , Ligon Keith L , Aldape Kenneth , Bulsara Ketan R , Johnson Kevin C , Alfaro Kristin D , Poisson Laila M , Garofano Luciano , Stead Lucy F , Nasrallah MacLean P , Smits Marion , van den Bent Martin J , Kouwenhoven Mathilde CM , Weller Michael , Hasanain Mohammad , Khasraw Mustafa , Gould Peter V , Smitt Peter A Sillevis , LaViolette Peter S , Tatman Philip D , Wesseling Pieter , French Pim J , Beroukhim Rameen , Verhaak Roel G.W. , Migliozzi Simona , Niclou Simone P , Bakas Spyridon , Kalkanis Steven , Paek Sun Ha , Short Susan C , Ghazaleh Tabatabai , Malta Tathiane M , Sabedot Thais S , Weiss Tobias , Walbert Tobias , Baid Ujjwal , Vallentgoed Wies , Yung W. K. Alfred , Verhaak Roel G.W. , Iavarone Antonio , Noushmehr Houtan
Published: March 2024

Table S5: Treatment-related probes and samples (Related to Figure 3)
S5A: List of 69 IDHmut pairs with treatment information (ID of Initial and Recurrent samples and group assignment)
S5B: List of differentially methylated probes associated with treatment in IDHmut gliomas
S5C: List of differentially methylated probes associated with treatment in IDHmut astrocytomas
S5D: List of CpG-gene pairs (epigenetic regulation associated with treatment)
Table S4 from The Epigenetic Evolution of Glioma Is Determined by the IDH1 Mutation Status and Treatment Regimen
Authors: Malta Tathiane M. , Sabedot Thais S. , Morosini Natalia S. , Datta Indrani , Garofano Luciano , Vallentgoed Wies , Varn Frederick S. , Aldape Kenneth , D'Angelo Fulvio , Bakas Spyridon , Barnholtz-Sloan Jill S. , Gan Hui K. , Hasanain Mohammad , Hau Ann-Christin , Johnson Kevin C. , Cazacu Simona , deCarvalho Ana C. , Khasraw Mustafa , Kocakavuk Emre , Kouwenhoven Mathilde C.M. , Migliozzi Simona , Niclou Simone P. , Niers Johanna M. , Ormond D. Ryan , Paek Sun Ha , Reifenberger Guido , Sillevis Smitt Peter A. , Smits Marion , Stead Lucy F. , van den Bent Martin J. , Van Meir Erwin G. , Walenkamp Annemiek , Weiss Tobias , Weller Michael , Westerman Bart A. , Ylstra Bauke , Wesseling Pieter , Lasorella Anna , French Pim J. , Poisson Laila M. , Woehrer Adelheid , Lowman Allison K , deCarvalho Ana C , Castro Ana Valeria , Transou Andrea , Brodbelt Andrew R , Hau Ann-Christin , Lasorella Anna , Golebiewska Anna , Walenkamp Annemiek , Molinaro Annette M , Iavarone Antonio , Ismail Azzam , Westerman Bart A , Ylstra Bauke , Bock Christoph , Ormond D. Ryan , Brat Daniel J , Kocakavuk Emre , Van Meir Erwin G , Barthel Floris P , Varn Frederick S , D'Angelo Fulvio , Finocchiaro Gaetano , Rao Ganesh , Zadeh Gelareh , Reifenberger Guido , ngNg Ho Keu , Kim Hoon , Noushmehr Houtan , Miletic Hrvoje , Gan Hui K , Datta Indrani , Rock Jack , Snyder James M , Huse Jason T , Connelly Jennifer M , Barnholtz-Sloan Jill S , Niers Johanna M , deGroot John F , Akdemir Kadir C , Kannan Kasthuri S , Ligon Keith L , Aldape Kenneth , Bulsara Ketan R , Johnson Kevin C , Alfaro Kristin D , Poisson Laila M , Garofano Luciano , Stead Lucy F , Nasrallah MacLean P , Smits Marion , van den Bent Martin J , Kouwenhoven Mathilde CM , Weller Michael , Hasanain Mohammad , Khasraw Mustafa , Gould Peter V , Smitt Peter A Sillevis , LaViolette Peter S , Tatman Philip D , Wesseling Pieter , French Pim J , Beroukhim Rameen , Verhaak Roel G.W. , Migliozzi Simona , Niclou Simone P , Bakas Spyridon , Kalkanis Steven , Paek Sun Ha , Short Susan C , Ghazaleh Tabatabai , Malta Tathiane M , Sabedot Thais S , Weiss Tobias , Walbert Tobias , Baid Ujjwal , Vallentgoed Wies , Yung W. K. Alfred , Verhaak Roel G.W. , Iavarone Antonio , Noushmehr Houtan
Published: March 2024

Table S4: Master regulators - list of samples and features (Related to Figure 2)
S4A: List of sample profiled for ChIP-seq
S4B: Results of H3K4me3 differential analysis
S4C: Results of H3K27Ac differential analysis
S4D: Results of gene expression differential analysis
S4E: List of IDHmut non-codels samples profiled for both gene expression and DNA methylation
S4F: List of hypomethylated probes between IDHmut non-codel initial and first recurrent pairs
S4G: List of anticorrelated genes
S4H: List of HOXD13 predicted targets
Supplementary Figure3 from Adipocyte Precursor-Derived NRG1 Promotes Resistance to FGFR Inhibition in Urothelial Carcinoma
Authors: Hosni Sana , Kilian Viola , Klümper Niklas , Gabbia Daniela , Sieckmann Katharina , Corvino Dillon , Winkler Anja , Saponaro Miriam , Wörsdörfer Karin , Schmidt Doris , Hahn Oliver , Zanotto Ilaria , Bertlich Marina , Toma Marieta , Bald Tobias , Eckstein Markus , Hölzel Michael , Geyer Matthias , Ritter Manuel , Wachten Dagmar , De Martin Sara , Alajati Abdullah
Published: March 2024

Supplementary Figure3
Adipose Stromal Cell–Derived Cancer-Associated Fibroblasts Suppress FGFR Inhibitor Efficacy
Authors: Kolonin Mikhail G. , Anastassiou Dimitris
Published: March 2024

Cancer aggressiveness has been linked with obesity, and studies have shown that adipose tissue can enhance cancer progression. In this issue of Cancer Research, Hosni and colleagues discover a paracrine mechanism mediated by adipocyte precursor cells through which urothelial carcinomas become resistant to erdafitinib, a recently approved therapy inhibiting fibroblast growth factor receptors (FGFR). They identified neuregulin 1 (NRG1) secreted by adipocyte precursor cells as an activator of HER3 signaling that enables resistance. The NRG1-mediated FGFR inhibitor resistance was amenable to intervention with pertuzumab, an antibody blocking the NRG1/HER3 axis. To investigate the nature of the resistance-associated NRG1-expressing cells in human patients, the authors analyzed published single-cell RNA sequencing data and observed that such cells appear in a cluster assigned as inflammatory cancer-associated fibroblasts (iCAF). Notably, the gene signature corresponding to these CAFs is highly similar to that shared by adipose stromal cells (ASC) in fat tissue and fibro-adipogenic progenitors (FAP) in skeletal muscle of cancer-free individuals. Because fibroblasts with the ASC/FAP signature are enriched in various carcinomas, it is possible that the paracrine signaling conferred by NRG1 is a pan-cancer mechanism of FGFR inhibitor resistance and tumor aggressiveness.

See related article by Hosni et al., p. 725
A Pre-Leukemic DNA Methylation Signature in Healthy Individuals at Higher Risk for Developing Myeloid Malignancy
Authors: Lao Zhentang , Ding Ling-Wen , Sun Qiao-Yang , Jia Li , Yan Benedict , Ng Alvin Yu-Jin , Capinpin Sharah Mae , Wang Renwei , Ying Li , Chng Wee Joo , Koeffler H. Phillip , Koh Woon-Puay , Yuan Jian-Min , Yang Henry , Goh Yeow Tee , Grigoropoulos Nicholas
Published: March 2024

Purpose:

DNA methylation alterations are widespread in acute myelogenous leukemia (AML) and myelodysplastic syndrome (MDS), some of which appear to have evolved independently of somatic mutations in epigenetic regulators. Although the presence of somatic mutations in peripheral blood can predict the risk of development of AML and MDS, its accuracy remains unsatisfactory.

Experimental Design:

We performed global DNA methylation profiling in a case control study nested within the Singapore Chinese Health Study to evaluate whether DNA methylation alterations were associated with AML/MDS development. Targeted deep sequencing and methylated DNA immunoprecipitation sequencing (MeDIP-seq) were performed on peripheral blood collected a median of 9.9 years before diagnosis of AML or MDS, together with age-matched still-healthy individuals as controls.

Results:

Sixty-six individuals who developed AML or MDS displayed significant DNA methylation changes in the peripheral blood compared with 167 age- and gender-matched controls who did not develop AML/MDS during the follow-up period. Alterations in methylation in the differentially methylation regions were associated with increased odds of developing AML/MDS.

Conclusions:

The epigenetic changes may be acquired independently and before somatic mutations that are relevant for AML/MDS development. The association between methylation changes and the risk of pre-AML/MDS in these individuals was considerably stronger than somatic mutations, suggesting that methylation changes could be used as biomarkers for pre-AML/MDS screening.
The Irreversible FGFR Inhibitor KIN-3248 Overcomes FGFR2 Kinase Domain Mutations
Authors: Balasooriya Eranga R. , Wu Qibiao , Ellis Haley , Zhen Yuanli , Norden Bryanna L. , Corcoran Ryan B. , Mohan Adithi , Martin Eric , Franovic Aleksandra , Tyhonas John , Lardy Matthew , Grandinetti Kathryn B. , Pelham Robert , Soroceanu Liliana , Silveira Vanessa S. , Bardeesy Nabeel
Published: March 2024

Purpose:

FGFR2 and FGFR3 show oncogenic activation in many cancer types, often through chromosomal fusion or extracellular domain mutation. FGFR2 and FGFR3 alterations are most prevalent in intrahepatic cholangiocarcinoma (ICC) and bladder cancers, respectively, and multiple selective reversible and covalent pan-FGFR tyrosine kinase inhibitors (TKI) have been approved in these contexts. However, resistance, often due to acquired secondary mutations in the FGFR2/3 kinase domain, limits efficacy. Resistance is typically polyclonal, involving a spectrum of different mutations that most frequently affect the molecular brake and gatekeeper residues (N550 and V565 in FGFR2).

Experimental Design:

Here, we characterize the activity of the next-generation covalent FGFR inhibitor, KIN-3248, in preclinical models of FGFR2 fusion+ ICC harboring a series of secondary kinase domain mutations, in vitro and in vivo. We also test select FGFR3 alleles in bladder cancer models.

Results:

KIN-3248 exhibits potent selectivity for FGFR1–3 and retains activity against various FGFR2 kinase domain mutations, in addition to being effective against FGFR3 V555M and N540K mutations. Notably, KIN-3248 activity extends to the FGFR2 V565F gatekeeper mutation, which causes profound resistance to currently approved FGFR inhibitors. Combination treatment with EGFR or MEK inhibitors potentiates KIN-3248 efficacy in vivo, including in models harboring FGFR2 kinase domain mutations.

Conclusions:

Thus, KIN-3248 is a novel FGFR1–4 inhibitor whose distinct activity profile against FGFR kinase domain mutations highlights its potential for the treatment of ICC and other FGFR-driven cancers.
Table S3 from ERBB4-Mediated Signaling Is a Mediator of Resistance to PI3K and BTK Inhibitors in B-cell Lymphoid Neoplasms
Authors: Arribas Alberto J. , Napoli Sara , Cascione Luciano , Barnabei Laura , Sartori Giulio , Cannas Eleonora , Gaudio Eugenio , Tarantelli Chiara , Mensah Afua A. , Spriano Filippo , Zucchetto Antonella , Rossi Francesca M. , Rinaldi Andrea , Castro de Moura Manuel , Jovic Sandra , Bordone Pittau Roberta , Stathis Anastasios , Stussi Georg , Gattei Valter , Brown Jennifer R. , Esteller Manel , Zucca Emanuele , Rossi Davide , Bertoni Francesco
Published: March 2024

Panel of proteins tested in the immunoblotting or immunofluorescence experiments.
Supplementary Table 7 from ERBB4-Mediated Signaling Is a Mediator of Resistance to PI3K and BTK Inhibitors in B-cell Lymphoid Neoplasms
Authors: Arribas Alberto J. , Napoli Sara , Cascione Luciano , Barnabei Laura , Sartori Giulio , Cannas Eleonora , Gaudio Eugenio , Tarantelli Chiara , Mensah Afua A. , Spriano Filippo , Zucchetto Antonella , Rossi Francesca M. , Rinaldi Andrea , Castro de Moura Manuel , Jovic Sandra , Bordone Pittau Roberta , Stathis Anastasios , Stussi Georg , Gattei Valter , Brown Jennifer R. , Esteller Manel , Zucca Emanuele , Rossi Davide , Bertoni Francesco
Published: March 2024

Clinical information on the series of serum samples from CLL clinical patients exposed to idelalisib or to ibrutinib. Samples were longitudinally acquired at different time points. Patients were paired according to similar clinical features.
Supplementary Table 6 from ERBB4-Mediated Signaling Is a Mediator of Resistance to PI3K and BTK Inhibitors in B-cell Lymphoid Neoplasms
Authors: Arribas Alberto J. , Napoli Sara , Cascione Luciano , Barnabei Laura , Sartori Giulio , Cannas Eleonora , Gaudio Eugenio , Tarantelli Chiara , Mensah Afua A. , Spriano Filippo , Zucchetto Antonella , Rossi Francesca M. , Rinaldi Andrea , Castro de Moura Manuel , Jovic Sandra , Bordone Pittau Roberta , Stathis Anastasios , Stussi Georg , Gattei Valter , Brown Jennifer R. , Esteller Manel , Zucca Emanuele , Rossi Davide , Bertoni Francesco
Published: March 2024

Multi-omics analyses. Output tables of the moderated t-tests comparing transcriptome, microRNA and methylation profiles of resistant and parental. Output results of Gene Set Enrichment Analysis comparing GEP data of resistant and parental.
Supplementary Table 5 from ERBB4-Mediated Signaling Is a Mediator of Resistance to PI3K and BTK Inhibitors in B-cell Lymphoid Neoplasms
Authors: Arribas Alberto J. , Napoli Sara , Cascione Luciano , Barnabei Laura , Sartori Giulio , Cannas Eleonora , Gaudio Eugenio , Tarantelli Chiara , Mensah Afua A. , Spriano Filippo , Zucchetto Antonella , Rossi Francesca M. , Rinaldi Andrea , Castro de Moura Manuel , Jovic Sandra , Bordone Pittau Roberta , Stathis Anastasios , Stussi Georg , Gattei Valter , Brown Jennifer R. , Esteller Manel , Zucca Emanuele , Rossi Davide , Bertoni Francesco
Published: March 2024

Whole exome sequencing data, including single nucleotide variants and copy number variants.
Figure S7 from ERBB4-Mediated Signaling Is a Mediator of Resistance to PI3K and BTK Inhibitors in B-cell Lymphoid Neoplasms
Authors: Arribas Alberto J. , Napoli Sara , Cascione Luciano , Barnabei Laura , Sartori Giulio , Cannas Eleonora , Gaudio Eugenio , Tarantelli Chiara , Mensah Afua A. , Spriano Filippo , Zucchetto Antonella , Rossi Francesca M. , Rinaldi Andrea , Castro de Moura Manuel , Jovic Sandra , Bordone Pittau Roberta , Stathis Anastasios , Stussi Georg , Gattei Valter , Brown Jennifer R. , Esteller Manel , Zucca Emanuele , Rossi Davide , Bertoni Francesco
Published: March 2024

(A) Immunoblotting was performed to measure protein levels in parental and resistant clones for pAKT/AKT and pERK/ERK in three independent experiments. GAPDH was used as a loading control. (B) Protein quantification was done normalizing first to GAPDH and then to total protein. Barplot represents the quantification in each of the independent experiments, parental in grey, resistant in red. Median and standard error of the mean for parental and resistant levels in the three experiments. Moderated t-test was performed to determine statistically significance (p<0.05).
Figure S6 from ERBB4-Mediated Signaling Is a Mediator of Resistance to PI3K and BTK Inhibitors in B-cell Lymphoid Neoplasms
Authors: Arribas Alberto J. , Napoli Sara , Cascione Luciano , Barnabei Laura , Sartori Giulio , Cannas Eleonora , Gaudio Eugenio , Tarantelli Chiara , Mensah Afua A. , Spriano Filippo , Zucchetto Antonella , Rossi Francesca M. , Rinaldi Andrea , Castro de Moura Manuel , Jovic Sandra , Bordone Pittau Roberta , Stathis Anastasios , Stussi Georg , Gattei Valter , Brown Jennifer R. , Esteller Manel , Zucca Emanuele , Rossi Davide , Bertoni Francesco
Published: March 2024

Expression levels of surface ERBB4 and pERBB4 was measured by FACS (A) and immunoblotting (B) in Karpas1718 parental and resistant. (A) Two independent experiments were performed: exp1 (left panel) and exp2 (right panel). Density plots show the median MFI values of two replicates from each experiment: negative control (dotted black), parental (grey), resistant (red). Results are presented from Welch Two Sample test performed on MFI values of the two experiments. (B) Immunobloting for total (grey) and phospho (red) ERBB4 in parental (left) and resistant (right) Karpas1718 cells. Representative image of two independent experiments. Data on the barplot represents the mean of two experiments, errors bars represent standard deviation of the mean. * for p<0.05 from a t-test.