bluuebunny/crossref_metadata_2025 huggingface dataset:
IGF-1 and Glucocorticoid Receptors Are Potential Target Proteins for the NGF-Mimic Effect of β-Cyclocitral from Lavandula angustifolia Mill. in PC12 Cells
Authors: An Chenyue , Gao Lijuan , Xiang Lan , Qi Jianhua
Published: September 2024

In the present study, the PC12 cells as a bioassay system were used to screen the small molecules with nerve growth factor (NGF)- mimic effect from Lavandula angustifolia Mill. The β-Cyclocitral (β-cyc) as an active compound was discovered, and its chemical structure was also determined. Furthermore, we focused on the bioactive and action mechanism of this compound to do an intensive study with specific protein inhibitors and Western blotting analysis. The β-cyc had novel NGF-mimic and NGF-enhancer effects on PC12 cells, while the insulin-like growth factor-1 receptor (IGF-1R)/phosphatidylinositol 3 kinase, (PI3K)/serine/threonine-protein kinase (AKT), and glucocorticoid receptor (GR)/phospholipase C (PLC)/protein kinase C (PKC) signaling pathways were involved in the bioactivity of β-cyc. In addition, the important role of the rat sarcoma (Ras)/protooncogene serine-threonine protein kinase (Raf) signaling pathway was observed, although it was independent of tyrosine kinase (Trk) receptors. Moreover, the non-label target protein discovery techniques, such as the cellular thermal shift assay (CETSA) and drug affinity responsive target stability (DARTS), were utilized to make predictions of its target protein. The stability of IGF-R and GR, proteins for temperature and protease, was dose-dependently increased after treatment of β-cyc compared with control groups, respectively. These findings indicated that β-cyc promoted the neuron differentiation of PC12 cells via targeting IGF-1R and GR and modification of downstream signaling pathways.
Thyroid Malignancy and Cutaneous Lichen Amyloidosis: Key Points Amid RET Pathogenic Variants in Medullary Thyroid Cancer/Multiple Endocrine Neoplasia Type 2 (MEN2)
Authors: Stanescu Laura-Semonia , Ghemigian Adina , Ciobica Mihai-Lucian , Nistor Claudiu , Ciuche Adrian , Radu Andreea-Maria , Sandru Florica , Carsote Mara
Published: September 2024

We aimed to provide an updated narrative review with respect to the RET pathogenic variants and their implications at the clinical and molecular level in the diagnosis of medullary thyroid cancer (MTC)/multiple endocrine neoplasia (MEN) type 2, particularly with respect to the presence of cutaneous lichen amyloidosis (CLA). We searched English-language, in extenso original articles with no timeline nor study design restriction that were published on PubMed. A traditional interplay stands for CLA and MTC in MEN2 (not MEN3) confirmation. While the connection has been reported for more than three decades, there is still a large gap in understanding and addressing it. The majority of patients with MEN2A-CLA have RET pathogenic variants at codon 634; hence, it suggests an involvement of this specific cysteine residue in both disorders (most data agree that one-third of C634-positive subjects have CLA, but the ranges are between 9% and 50%). Females seem more prone to MEN2-CLA than males. Non-C634 germline RET pathogenic variants included (at a low level of statistical evidence) the following: RET V804M mutation in exon 14 for MTC-CLA (CLA at upper back); RET S891A mutation in exon 15 binding OSMR variant G513D (familial MTC and CLA comprising the lower legs to thighs, upper back, shoulders, arms, and forearms); and C611Y (CLA at interscapular region), respectively. Typically, CLA is detected at an early age (from childhood until young adulthood) before the actual MTC identification unless RET screening protocols are already applied. The time frame between CLA diagnosis and the identification of RET pathogenic variants was between 5 and 60 years according to one study. The same RET mutation in one family is not necessarily associated with the same CLA presentation. In MTC/MEN2 subjects, the most affected CLA area was the scapular region of the upper back. Alternatively, another hypothesis highlighted the fact that CLA is secondary to long-term prurit/notalgia paresthetica (NP) in MTC/MEN2. OSMR p. G513D may play a role in modifying the evolutionary processes of CLA in subjects co-harboring RET mutations (further studies are necessary to sustain this aspect). Awareness in CLA-positive patients is essential, including the decision of RET testing in selected cases.
Mechanistic Insights into Substrate Recognition of Human Nucleoside Diphosphate Kinase C Based on Nucleotide-Induced Structural Changes
Authors: Amjadi Rezan , Werten Sebastiaan , Lomada Santosh Kumar , Baldin Clara , Scheffzek Klaus , Dunzendorfer-Matt Theresia , Wieland Thomas
Published: September 2024

Nucleoside diphosphate kinases (NDPKs) are encoded by nme genes and exist in various isoforms. Based on interactions with other proteins, they are involved in signal transduction, development and pathological processes such as tumorigenesis, metastasis and heart failure. In this study, we report a 1.25 Å resolution structure of human homohexameric NDPK-C bound to ADP and describe the yet unknown complexes formed with GDP, UDP and cAMP, all obtained at a high resolution via X-ray crystallography. Each nucleotide represents a distinct group of mono- or diphosphate purine or pyrimidine bases. We analyzed different NDPK-C nucleotide complexes in the presence and absence of Mg2+ and explain how this ion plays an essential role in NDPKs’ phosphotransferase activity. By analyzing a nucleotide-depleted NDPK-C structure, we detected conformational changes upon substrate binding and identify flexible regions in the substrate binding site. A comparison of NDPK-C with other human isoforms revealed a strong similarity in the overall composition with regard to the 3D structure, but significant differences in the charge and hydrophobicity of the isoforms’ surfaces. This may play a role in isoform-specific NDPK interactions with ligands and/or important complex partners like other NDPK isoforms, as well as monomeric and heterotrimeric G proteins. Considering the recently discovered role of NDPK-C in different pathologies, these high-resolution structures thus might provide a basis for interaction studies with other proteins or small ligands, like activators or inhibitors.
Role of Divalent Cations in Infections in Host–Pathogen Interaction
Authors: D’Elia John A. , Weinrauch Larry A.
Published: September 2024

With increasing numbers of patients worldwide diagnosed with diabetes mellitus, renal disease, and iatrogenic immune deficiencies, an increased understanding of the role of electrolyte interactions in mitigating pathogen virulence is necessary. The levels of divalent cations affect host susceptibility and pathogen survival in persons with relative immune insufficiency. For instance, when host cellular levels of calcium are high compared to magnesium, this relationship contributes to insulin resistance and triples the risk of clinical tuberculosis. The movement of divalent cations within intracellular spaces contributes to the host defense, causing apoptosis or autophagy of the pathogen. The control of divalent cation flow is dependent in part upon the mammalian natural resistance-associated macrophage protein (NRAMP) in the host. Survival of pathogens such as M tuberculosis within the bronchoalveolar macrophage is also dependent upon NRAMP. Pathogens evolve mutations to control the movement of calcium through external and internal channels. The host NRAMP as a metal transporter competes for divalent cations with the pathogen NRAMP in M tuberculosis (whether in latent, dormant, or active phase). This review paper summarizes mechanisms of pathogen offense and patient defense using inflow and efflux through divalent cation channels under the influence of parathyroid hormone vitamin D and calcitonin.
Prenatal Stress and Ethanol Exposure: Microbiota-Induced Immune Dysregulation and Psychiatric Risks
Authors: Camarini Rosana , Marianno Priscila , Hanampa-Maquera Maylin , Oliveira Samuel dos Santos , Câmara Niels Olsen Saraiva
Published: September 2024

Changes in maternal gut microbiota due to stress and/or ethanol exposure can have lasting effects on offspring’s health, particularly regarding immunity, inflammation response, and susceptibility to psychiatric disorders. The literature search for this review was conducted using PubMed and Scopus, employing keywords and phrases related to maternal stress, ethanol exposure, gut microbiota, microbiome, gut–brain axis, diet, dysbiosis, progesterone, placenta, prenatal development, immunity, inflammation, and depression to identify relevant studies in both preclinical and human research. Only a limited number of reviews were included to support the arguments. The search encompassed studies from the 1990s to the present. This review begins by exploring the role of microbiota in modulating host health and disease. It then examines how disturbances in maternal microbiota can affect the offspring’s immune system. The analysis continues by investigating the interplay between stress and dysbiosis, focusing on how prenatal maternal stress influences both maternal and offspring microbiota and its implications for susceptibility to depression. The review also considers the impact of ethanol consumption on gut dysbiosis, with an emphasis on the effects of prenatal ethanol exposure on both maternal and offspring microbiota. Finally, it is suggested that maternal gut microbiota dysbiosis may be significantly exacerbated by the combined effects of stress and ethanol exposure, leading to immune system dysfunction and chronic inflammation, which could increase the risk of depression in the offspring. These interactions underscore the potential for novel mental health interventions that address the gut–brain axis, especially in relation to maternal and offspring health.
Discriminating Benign from Malignant Lung Diseases Using Plasma Glycosaminoglycans and Cell-Free DNA
Authors: Qvick Alvida , Bratulic Sinisa , Carlsson Jessica , Stenmark Bianca , Karlsson Christina , Nielsen Jens , Gatto Francesco , Helenius Gisela
Published: September 2024

We aimed to investigate the use of free glycosaminoglycan profiles (GAGomes) and cfDNA in plasma to differentiate between lung cancer and benign lung disease, in a cohort of 113 patients initially suspected of lung cancer. GAGomes were analyzed in all samples using the MIRAM® Free Glycosaminoglycan Kit with ultra-high-performance liquid chromatography and electrospray ionization triple quadrupole mass spectrometry. In a subset of samples, cfDNA concentration and NGS-data was available. We detected two GAGome features, 0S chondroitin sulfate (CS), and 4S CS, with cancer-specific changes. Based on the observed GAGome changes, we devised a model to predict lung cancer. The model, named the GAGome score, could detect lung cancer with 41.2% sensitivity (95% CI: 9.2–54.2%) at 96.4% specificity (95% CI: 95.2–100.0%, n = 113). When we combined the GAGome score with a cfDNA-based model, the sensitivity increased from 42.6% (95% CI: 31.7–60.6%, cfDNA alone) to 70.5% (95% CI: 57.4–81.5%) at 95% specificity (95% CI: 75.1–100%, n = 74). Notably, the combined GAGome and cfDNA testing improved the sensitivity, compared to cfDNA alone, especially in ASCL stage I (55.6% vs 11.1%). Our findings show that plasma GAGome profiles can enhance cfDNA testing performance, highlighting the applicability of a multiomics approach in lung cancer diagnostics.
Comparison of Mitochondrial and Antineoplastic Effects of Amiodarone and Desethylamiodarone in MDA-MB-231 Cancer Line
Authors: Ramadan Fadi H. J. , Koszegi Balazs , Vantus Viola B. , Fekete Katalin , Kiss Gyongyi N. , Rizsanyi Balint , Bognar Rita , Gallyas Ferenc , Bognar Zita
Published: September 2024

Previously, we have demonstrated that amiodarone (AM), a widely used antiarrhythmic drug, and its major metabolite desethylamiodarone (DEA) both affect several mitochondrial processes in isolated heart and liver mitochondria. Also, we have established DEA’s antitumor properties in various cancer cell lines and in a rodent metastasis model. In the present study, we compared AM’s and DEA’s mitochondrial and antineoplastic effects in a human triple-negative breast cancer (TNBC) cell line. Both compounds reduced viability in monolayer and sphere cultures and the invasive growth of the MDA-MB-231 TNBC line by inducing apoptosis. They lowered mitochondrial trans-membrane potential, increased Ca2+ influx, induced mitochondrial permeability transition, and promoted mitochondrial fragmentation. In accordance with their mitochondrial effects, both substances massively decreased overall, and even to a greater extent, mitochondrial ATP production decreased, as determined using a Seahorse live cell respirometer. In all these effects, DEA was more effective than AM, indicating that DEA may have higher potential in the therapy of TNBC than its parent compound.
Pathophysiology of Arginases in Cancer and Efforts in Their Pharmacological Inhibition
Authors: Marzęta-Assas Patrycja , Jacenik Damian , Zasłona Zbigniew
Published: September 2024

Arginases are key enzymes that hydrolyze L-arginine to urea and L-ornithine in the urea cycle. The two arginase isoforms, arginase 1 (ARG1) and arginase 2 (ARG2), regulate the proliferation of cancer cells, migration, and apoptosis; affect immunosuppression; and promote the synthesis of polyamines, leading to the development of cancer. Arginases also compete with nitric oxide synthase (NOS) for L-arginine, and their participation has also been confirmed in cardiovascular diseases, stroke, and inflammation. Due to the fact that arginases play a crucial role in the development of various types of diseases, finding an appropriate candidate to inhibit the activity of these enzymes would be beneficial for the therapy of many human diseases. In this review, based on numerous experimental, preclinical, and clinical studies, we provide a comprehensive overview of the biological and physiological functions of ARG1 and ARG2, their molecular mechanisms of action, and affected metabolic pathways. We summarize the recent clinical trials’ advances in targeting arginases and describe potential future drugs.
Calcium Role in Gap Junction Channel Gating: Direct Electrostatic or Calmodulin-Mediated?
Authors: Peracchia Camillo
Published: September 2024

The chemical gating of gap junction channels is mediated by cytosolic calcium (Ca2+i) at concentrations ([Ca2+]i) ranging from high nanomolar (nM) to low micromolar (µM) range. Since the proteins of gap junctions, connexins/innexins, lack high-affinity Ca2+-binding sites, most likely gating is mediated by a Ca2+-binding protein, calmodulin (CaM) being the best candidate. Indeed, the role of Ca2+-CaM in gating is well supported by studies that have tested CaM blockers, CaM expression inhibition, testing of CaM mutants, co-localization of CaM and connexins, existence of CaM-binding sites in connexins/innexins, and expression of connexins (Cx) mutants, among others. Based on these data, since 2000, we have published a Ca2+-CaM-cork gating model. Despite convincing evidence for the Ca2+-CaM role in gating, a recent study has proposed an alternative gating model that would involve a direct electrostatic Ca2+-connexin interaction. However, this study, which tested the effect of unphysiologically high [Ca2+]i on the structure of isolated junctions, reported that neither changes in the channel’s pore diameter nor connexin conformational changes are present, in spite of exposure of isolated gap junctions to [Ca2+]i as high at the 20 mM. In conclusion, data generated in the past four decades by multiple experimental approaches have clearly demonstrated the direct role of Ca2+-CaM in gap junction channel gating.
Thermodynamic Factors Controlling Electron Transfer among the Terminal Electron Acceptors of Photosystem I: Insights from Kinetic Modelling
Authors: Santabarbara Stefano , Casazza Anna Paola
Published: September 2024

Photosystem I is a key component of primary energy conversion in oxygenic photosynthesis. Electron transfer reactions in Photosystem I take place across two parallel electron transfer chains that converge after a few electron transfer steps, sharing both the terminal electron acceptors, which are a series of three iron–sulphur (Fe-S) clusters known as FX, FA, and FB, and the terminal donor, P700. The two electron transfer chains show kinetic differences which are, due to their close geometrical symmetry, mainly attributable to the tuning of the physicochemical reactivity of the bound cofactors, exerted by the protein surroundings. The factors controlling the rate of electron transfer between the terminal Fe-S clusters are still not fully understood due to the difficulties of monitoring these events directly. Here we present a discussion concerning the driving forces associated with electron transfer between FX and FA as well as between FA and FB, employing a tunnelling-based description of the reaction rates coupled with the kinetic modelling of forward and recombination reactions. It is concluded that the reorganisation energy for FX− oxidation shall be lower than 1 eV. Moreover, it is suggested that the analysis of mutants with altered FA redox properties can also provide useful information concerning the upstream phylloquinone cofactor energetics.
Critical Care Ultrasound in Shock: A Comprehensive Review of Ultrasound Protocol for Hemodynamic Assessment in the Intensive Care Unit
Authors: Pérez Camilo , Diaz-Caicedo Diana , Almanza Hernández David Fernando , Moreno-Araque Lorena , Yepes Andrés Felipe , Carrizosa Gonzalez Jorge Armando
Published: September 2024

Shock is a life-threatening condition that requires prompt recognition and treatment to prevent organ failure. In the intensive care unit, shock is a common presentation, and its management is challenging. Critical care ultrasound has emerged as a reliable and reproducible tool in diagnosing and classifying shock. This comprehensive review proposes an ultrasound-based protocol for the hemodynamic assessment of shock to guide its management in the ICU. The protocol classifies shock as either low or high cardiac index and differentiates obstructive, hypovolemic, cardiogenic, and distributive etiologies. In distributive shock, the protocol proposes a hemodynamic-based approach that considers the presence of dynamic obstruction, fluid responsiveness, fluid tolerance, and ventriculo-arterial coupling. The protocol gives value to quantitative measures based on critical care ultrasound to guide hemodynamic management. Using critical care ultrasound for a comprehensive hemodynamic assessment can help clinicians diagnose the etiology of shock and define the appropriate treatment while monitoring the response. The protocol’s use in the ICU can facilitate prompt recognition, diagnosis, and management of shock, ultimately improving patient outcomes.
Redo Thyroidectomy: Updated Insights
Authors: Suveica Luminita , Sima Oana-Claudia , Ciobica Mihai-Lucian , Nistor Claudiu , Cucu Anca-Pati , Costachescu Mihai , Ciuche Adrian , Nistor Tiberiu Vasile Ioan , Carsote Mara
Published: September 2024

The risk of post-operatory hypothyroidism and hypocalcaemia, along with recurrent laryngeal nerve injury, is lower following a less-than-total thyroidectomy; however, a previously unsuspected carcinoma or a disease progression might be detected after initial surgery, hence indicating re-intervention as mandatory (so-called “redo” surgery) with completion. This decision takes into consideration a multidisciplinary approach, but the surgical technique and the actual approach is entirely based on the skills and availability of the surgical team according to the standard protocols regarding a personalised decision. We aimed to introduce a review of the most recently published data, with respect to redo thyroid surgery. For the basis of the discussion, a novel vignette on point was introduced. This was a narrative review. We searched English-language papers according to the key search terms in different combinations such as “redo” and “thyroid”, alternatively “thyroidectomy” and “thyroid surgery”, across the PubMed database. Inclusion criteria were original articles. The timeframe of publication was between 1 January 2020 and 20 July 2024. Exclusion criteria were non-English papers, reviews, non-human studies, case reports or case series, exclusive data on parathyroid surgery, and cell line experiments. We identified ten studies across the five-year most recent window of PubMed searches that showed a heterogeneous spectrum of complications and applications of different surgeries with respect to redo interventions during thyroid removal (e.g., recurrent laryngeal nerve monitoring during surgery, other types of incision than cervicotomy, the use of parathyroid fluorescence, bleeding risk, etc.). Most studies addressing novel surgical perspectives focused on robotic-assisted re-intervention, and an expansion of this kind of studies is expected. Further studies and multifactorial models of assessment and risk prediction are necessary to decide, assess, and recommend redo interventions and the most adequate surgical techniques.
Coronary Artery Aneurysms: A Clinical Case Report and Literature Review Supporting Therapeutic Choices
Authors: Sannino Michele , Nicolai Matteo , Infusino Fabio , Giulio Luciani , Usai Tommaso Leo , Biscotti Giovanni , Azzarri Alessandro , De Angelis D’Ossat Marina , Calcagno Sergio , Calcagno Simone
Published: September 2024

Coronary artery aneurysms (CAAs) are uncommon but significant cardiovascular abnormalities characterized by an abnormal increase in vascular diameter. CAAs are classified based on their shape as either saccular or fusiform, and their causes can range from atherosclerosis, Kawasaki disease, to congenital and iatrogenic factors. CAAs often present asymptomatically, but when symptoms occur, they can include angina, myocardial infarction, or even sudden cardiac death due to intravascular thrombosis involving the CAA. Diagnosis is typically confirmed through coronary angiography, though CT and other imaging techniques can provide additional details. The management of CAAs is variable depending on their size, location, and the presence of symptoms or complications. Treatment options include medical therapy, percutaneous coronary intervention (PCI), or surgical approaches. In this paper, we describe the case report of a 79-year-old male who presented with palpitations and was diagnosed with a right coronary artery aneurysm, and a review of the literature is delineated, underscoring the importance of individualized treatment strategies for CAAs.
Predictor of Surgical Failure Following Transvaginal Mesh Repair Using Minimally Invasive Prolapse System Device (MIPS)
Authors: Yang Yao-Yu , Loo Zi-Xi , Lin Kun-Ling , Long Cheng-Yu
Published: September 2024

Background: The Minimally Invasive Prolapse System (MIPS) device, a novel single-incision transvaginal mesh, represents recent advancements in mesh technology, providing lightweight, biocompatible support for pelvic organ prolapse while reducing erosion, allowing for customization and improving surgical outcomes. This study aimed to identify factors associated with pelvic organ prolapse (POP) recurrence after transvaginal mesh (TVM) repair using the Minimally Invasive Prolapse System device. Methods: Two hundred and eighteen women with symptomatic stage II to IV POP underwent TVM. Preoperative and postoperative assessments included urinalyses and pelvic examinations using the POP quantification (POP-Q) staging system. Results: During a follow-up period of 12–46 months, 7 of 218 (3.2%) women experienced POP recurrence. Univariate analysis was conducted to identify predictors of surgical failure, revealing no significant differences in body mass index, POP stage, or preoperative urinary symptoms between the recurrence and success groups (p > 0.05). However, functional urethral length <20 mm based on urodynamics (p = 0.011), ICI-Q scores ≥7 (p = 0.012), and the first 60 surgical cases (p = 0.018) were significant predictors of surgical failure. Multivariate logistic regression confirmed these findings. Conclusions: Functional urethral length <20 mm, ICI-Q scores ≥7, and limited surgical experience were significant predictors of TVM failure using the Minimally Invasive Prolapse System kit. POP recurrence after mesh repair is less likely beyond the learning curve.
A Prospective Randomized Control Trial of Lingual Frenuloplasty with Myofunctional Therapy in Patients with Maxillofacial Deformity in a Polish Cohort
Authors: Lichnowska Anna , Gnatek Adrian , Tyszkiewicz Szymon , Kozakiewicz Marcin , Zaghi Soroush
Published: September 2024

Introduction: There are few publications concerning ankyloglossia in mixed-aged groups utilizing myofunctional therapy and frenuloplasty in patients undergoing orthodontic treatment and maxillofacial surgery. While it is well known that ankyloglossia is mainly diagnosed in babies, research on functional and structural disorders in different age groups is less common. Thus, there is a high need for specific information about the influence and effectiveness of frenuloplasty with myofunctional therapy on the stomatognathic function and final treatment outcome for a wider variety of patients, especially those with maxillofacial deformities. Aim: This paper aims to evaluate the impact of lingual frenuloplasty as an adjunct to myofunctional therapy for the treatment of ankyloglossia in children and adults with maxillofacial deformity. Methods: Prospective randomized control trial with 155 subjects. Methods were based on visual observation and examination of the oral cavity. There were two groups: myofunctional therapy vs. myofunctional therapy and lingual frenuloplasty. Patients were randomized based on order of entry into the study. χ2 test, Kruskal–Wallis, ANOVA, Student’s t-test and others were used for statistical analyses. Results: The presented protocol with myofunctional therapy and surgical procedures proved to be significantly more effective in improving tongue mobility and stomatognathic functions such as swallowing, breathing, and oral resting postures as compared to the reference group who underwent myofunctional therapy only. Conclusions: Lingual frenuloplasty with myofunctional therapy is highly effective in restoring the equilibrium of the orofacial muscles and the skeleton, which is often disturbed and may lead to unstable functional effects among patients considering orthodontic and orthognathic treatments for maxillofacial deformities.
Favorable Outcome after Liver Transplantation in an Infant with Liver Failure Due to Deoxyguanosine Kinase Deficiency
Authors: Grama Alina , Benţa Gabriel , Niculae Alexandru , Mititelu Alexandra , Simu Claudia , Fufezan Otilia , Stephenne Xavier , Reding Raymond , de Magnee Catherine , Tambucci Roberto , Sokal Etienne , Pop Tudor
Published: September 2024

Introduction: Deoxyguanosine Kinase (DGUOK) deficiency is a very rare disorder characterized by liver dysfunction, neurological manifestations, and metabolic disorders secondary to severely reduced mitochondrial DNA content. These patients develop early-onset liver failure, and their liver transplantation (LT) indication remains debatable due to the possibility of neurological involvement. Case Report: We present the case of a 6-month-old female diagnosed with DGUOK deficiency who developed liver failure. At 9 months, she underwent a living-related LT with an initial favorable evolution under immunosuppression therapy with tacrolimus. Four months after LT, she presented two prolonged bacterial and Rotavirus enteritis episodes. She developed classical post-transplant complications (severe renal tubular acidosis type IV, secondary to the high tacrolimus level, and post-transplant lymphoproliferative disease) during these episodes. Her condition deteriorated progressively, with reversible hypotonia and significant weight loss. However, the neurological evaluation did not reveal any signs suggestive of the progression of the underlying disease. A few months later, her clinical features and laboratory parameters improved considerably. Conclusions: This case highlights the unpredictable evolution of children with LT for liver failure due to DGUOK deficiency.
Metaanalysis of Repetitive Transcranial Magnetic Stimulation (rTMS) Efficacy for OCD Treatment: The Impact of Stimulation Parameters, Symptom Subtype and rTMS-Induced Electrical Field
Authors: Dehghani-Arani Fateme , Kazemi Reza , Hallajian Amir-Homayun , Sima Sepehr , Boutimaz Samaneh , Hedayati Sepideh , Koushamoghadam Saba , Safarifard Razieh , Salehinejad Mohammad Ali
Published: September 2024

Background: Repetitive transcranial magnetic stimulation (rTMS) has recently demonstrated significant potential in treating obsessive-compulsive disorder (OCD). However, its effectiveness depends on various parameters, including stimulation parameters, OCD subtypes and electrical fields (EFs) induced by rTMS in targeted brain regions that are less studied. Methods: Using the PRISMA approach, we examined 27 randomized control trials (RCTs) conducted from 1985 to 2024 using rTMS for the treatment of OCD and conducted several meta-analyses to investigate the role of rTMS parameters, including the EFs induced by each rTMS protocol, and OCD subtypes on treatment efficacy. Results: A significant, medium effect size was found, favoring active rTMS (gPPC = 0.59, p < 0.0001), which was larger for the obsession subscale. Both supplementary motor area (SMA) rTMS (gPPC = 0.82, p = 0.048) and bilateral dorsolateral prefrontal cortex (DLPFC) rTMS (gPPC = 1.14, p = 0.04) demonstrated large effect sizes, while the right DLPFC showed a significant moderate effect size for reducing OCD severity (gPPC = 0.63, p = 0.012). These protocols induced the largest EFs in dorsal cognitive, ventral cognitive and sensorimotor circuits. rTMS protocols targeting DLPFC produced the strongest electrical fields in cognitive circuits, while pre-supplementary motor area (pre-SMA) and orbitofrontal cortex (OFC) rTMS protocols induced larger fields in regions linked to emotional and affective processing in addition to cognitive circuits. The pre-SMA rTMS modulated more circuits involved in OCD pathophysiology—sensorimotor, cognitive, affective, and frontolimbic—with larger electrical fields than the other protocols. Conclusions: While rTMS shows moderate overall clinical efficacy, protocols targeting ventral and dorsal cognitive and sensorimotor circuits demonstrate the highest potential. The pre-SMA rTMS appears to induce electrical fields in more circuits relevant to OCD pathophysiology.
Rehabilitation to Improve Outcomes after Cervical Spine Surgery: Narrative Review
Authors: Sakaguchi Tomoyoshi , Heyder Ahmed , Tanaka Masato , Uotani Koji , Omori Toshinori , Kodama Yuya , Takamatsu Kazuhiko , Yasuda Yosuke , Sugyo Atsushi , Takeda Masanori , Nakagawa Masami
Published: September 2024

Purpose: The increasing elderly patient population is contributing to the rising worldwide load of cervical spinal disorders, which is expected to result in a global increase in the number of surgical procedures in the foreseeable future. Cervical rehabilitation plays a crucial role in optimal recovery after cervical spine surgeries. Nevertheless, there is no agreement in the existing research regarding the most suitable postsurgical rehabilitation program. Consequently, this review assesses the ideal rehabilitation approach for adult patients following cervical spine operations. Materials and Methods: This review covers activities of daily living and encompasses diverse treatment methods, including physiotherapy, specialized tools, and guidance for everyday activities. The review is organized under three headings: (1) historical perspectives, (2) patient-reported functional outcomes, and (3) general and disease-specific rehabilitation. Results: Rehabilitation programs are determined on the basis of patient-reported outcomes, performance tests, and disease prognosis. CSM requires strengthening of the neck and shoulder muscles that have been surgically invaded. In contrast, the CCI requires mobility according to the severity of the spinal cord injury and functional prognosis. The goal of rehabilitation for CCTs, as for CCIs, is to achieve ambulation, but the prognosis and impact of cancer treatment must be considered. Conclusions: Rehabilitation of the cervical spine after surgery is essential for improving physical function and the ability to perform daily activities and enhancing overall quality of life. The rehabilitation process should encompass general as well as disease-specific exercises. While current rehabilitation protocols heavily focus on strengthening muscles, they often neglect the crucial aspect of spinal balance. Therefore, giving equal attention to muscle reinforcement and the enhancement of spinal balance following surgery on the cervical spine is vital.
Influence of Damping Plate Size on Pitch Motion Response of Floating Offshore Wind Turbine
Authors: Xu Jie , Wang Chuansheng , Li Jiawen , Jiang Yichen , Tang Guangyin , Yang Yi
Published: September 2024

For floating offshore wind turbines, a significant pitch and roll motion response of the platform can affect the acceleration and power generation of the nacelle. The damping plate is considered a type of attachment that can be used to reduce rotational motion, but research on its anti-rotational effect is limited. The objective of this work is to analyze the impact of installing damping plates and varying their sizes on the pitch motion response of semi-submersible platforms, while also proposing optimization strategies for damping plate design. Firstly, a comparison between numerical simulations and experimental measurements validates the accuracy of the CFD calculations. Subsequently, different sizes of damping plates are proposed for the platforms, followed by simulations under various conditions. Finally, comprehensive data analysis is conducted. The findings suggest that installing damping plates enhances both the platform’s added moment of inertia and damping coefficients while simultaneously amplifying its motion response in regular waves. Furthermore, increasing the size of damping plates leads to an increase in both the added moment of inertia and motion response for the platform, whereas the damping coefficient exhibits an initial increasing trend followed by a subsequent decrease. Ultimately, it is found that increasing the distance between damping plates and the free surface significantly reduces wave-induced loads on the platform.
DA-YOLOv7: A Deep Learning-Driven High-Performance Underwater Sonar Image Target Recognition Model
Authors: Chen Zhe , Xie Guohao , Deng Xiaofang , Peng Jie , Qiu Hongbing
Published: September 2024

Affected by the complex underwater environment and the limitations of low-resolution sonar image data and small sample sizes, traditional image recognition algorithms have difficulties achieving accurate sonar image recognition. The research builds on YOLOv7 and devises an innovative fast recognition model designed explicitly for sonar images, namely the Dual Attention Mechanism YOLOv7 model (DA-YOLOv7), to tackle such challenges. New modules such as the Omni-Directional Convolution Channel Prior Convolutional Attention Efficient Layer Aggregation Network (OA-ELAN), Spatial Pyramid Pooling Channel Shuffling and Pixel-level Convolution Bilat-eral-branch Transformer (SPPCSPCBiFormer), and Ghost-Shuffle Convolution Enhanced Layer Aggregation Network-High performance (G-ELAN-H) are central to its design, which reduce the computational burden and enhance the accuracy in detecting small targets and capturing local features and crucial information. The study adopts transfer learning to deal with the lack of sonar image samples. By pre-training the large-scale Underwater Acoustic Target Detection Dataset (UATD dataset), DA-YOLOV7 obtains initial weights, fine-tuned on the smaller Smaller Common Sonar Target Detection Dataset (SCTD dataset), thereby reducing the risk of overfitting which is commonly encountered in small datasets. The experimental results on the UATD, the Underwater Optical Target Detection Intelligent Algorithm Competition 2021 Dataset (URPC), and SCTD datasets show that DA-YOLOV7 exhibits outstanding performance, with mAP@0.5 scores reaching 89.4%, 89.9%, and 99.15%, respectively. In addition, the model maintains real-time speed while having superior accuracy and recall rates compared to existing mainstream target recognition models. These findings establish the superiority of DA-YOLOV7 in sonar image analysis tasks.
A Deep Learning Strategy for the Retrieval of Sea Wave Spectra from Marine Radar Data
Authors: Ludeno Giovanni , Esposito Giuseppe , Lugni Claudio , Soldovieri Francesco , Gennarelli Gianluca
Published: September 2024

In the context of sea state monitoring, reconstructing the wave field and estimating the sea state parameters from radar data is a challenging problem. To reach this goal, this paper proposes a fully data-driven, deep learning approach based on a convolutional neural network. The network takes as input the radar image spectrum and outputs the sea wave directional spectrum. After a 2D fast Fourier transform, the wave elevation field is reconstructed, and accordingly, the sea state parameters are estimated. The reconstruction strategy, herein presented, is tested using numerical data generated from a synthetic sea wave simulator, considering the spectral proprieties of the Joint North Sea Wave Observation Project model. A performance analysis of the proposed deep-learning estimation strategy is carried out, along with a comparison to the classical modulation transfer function approach. The results demonstrate that the proposed approach is effective in reconstructing the directional wave spectrum across different sea states.
Structural Design and Horizontal Wave Force Estimation of a Wall-Climbing Robot for the Underwater Cleaning of Jackets
Authors: Jiao Shilong , Zhang Xiaojun , Sun Lingyu , Shi Yusheng , Zhang Minglu
Published: September 2024

Currently, divers face significant safety risks when cleaning marine organisms from the steel structures of offshore underwater platform jackets. Consequently, utilizing robots instead of divers to carry out underwater biofouling removal operations will be an important development direction for the underwater maintenance of offshore platforms in the future. In this study, a wall-climbing robot was designed to clean marine organisms from the underwater surface of a platform jacket leg. The overall structure of the underwater cleaning wall-climbing robot is introduced, including the cleaning actuator and the variable curvature-adapted connecting rod mechanism. The corresponding relationship between the variable curvature-adapted connecting rod mechanism and the jacket leg is analyzed in detail. The variable curvature-adapted connecting rod mechanism was optimized using a genetic algorithm to ensure that the underwater cleaning wall-climbing robot can adapt to a minimum diameter of 1 m for the jacket leg. By drawing on Airy wave theory and random wave theory, the Airy wave parameters for waves were analyzed under different sea conditions, considering practical application scenarios. By using Fluent software 2022, a 2D numerical wave tank was constructed to simulate waves under various sea conditions, and the wave surface shapes for different sea states were determined. By building on the Morison equation, a method for calculating the horizontal wave forces on the underwater cleaning wall-climbing robot using the equivalent area and equivalent volume is proposed. By using the two aforementioned methods, the horizontal wave forces on the underwater cleaning wall-climbing robot under specific sea states were determined. The horizontal wave forces of the underwater cleaning wall-climbing robot under different sea conditions were analyzed and simulated in a 3D numerical wave tank. By comparing the theoretical analysis results with the numerical simulation results, where the maximum difference at the extreme points is approximately 11%, the feasibility of the proposed horizontal wave force estimation method was verified.
Sustainable Agritourism for Farm Profitability: Comprehensive Evaluation of Visitors’ Intrinsic Motivation, Environmental Behavior, and Satisfaction
Authors: Baby Jibin , Kim Dae-Young
Published: September 2024

Unstable farm income and the desire to diversify revenue sources have increased the significance of agritourism as an alternative economic opportunity for farmers and ranchers. Agritourism integrates the top economic drivers—agriculture and tourism—and has been identified as a highly effective complementary business for farmers to generate additional income and mitigate the financial uncertainties associated with traditional farming enterprises. Visitors’ satisfaction is critical for operating a successful agritourism business, as it influences destination choice, consumption of products and services, and the decision to return. This study examined the relationship between agritourism visitors’ intrinsic motivation, environmental behavior, satisfaction, and intentions to revisit and recommend. With a total of 615 survey responses, the study reveals a significant relationship between agritourism visitors’ intrinsic motivation, environmental behavior, and satisfaction related to destination, risk, and food attributes. Furthermore, visitors’ overall satisfaction with these three attributes significantly influences their intentions to revisit and recommend the destination. The findings of this study will enable agritourism operators and policymakers to formulate appropriate policies for the sustainable development of this sector. Future promotional and educational tools could be developed based on these findings.
Exploring the Impact of Visual and Aural Elements in Urban Parks on Human Behavior and Emotional Responses
Authors: Jin Tongfei , Lu Jiayi , Shao Yuhan
Published: September 2024

As cities progress into high-quality developments, the demand for urban parks that enhance residents’ well-being and sustainability is increasing. Traditional visual-centric design methods no longer suffice. Given that vision and hearing are the primary sensory pathways through which people perceive their environment, exploring their relationship with landscape experiences offers a novel perspective for optimizing the audiovisual perception quality of urban parks. This study explores the relationship between visual and auditory elements and landscape experiences to optimize urban parks’ sensory quality. Using visual perception, soundscape perception, sound source perception, and behavioral vitality, this study evaluates the audiovisual perception quality of a representative wetland park in Chengdu’s ring ecological zone. By quantifying relationships between audiovisual characteristics, behavioral vitality, and emotional feedback, several emotional assessment models were constructed. The results show that lawns, pavements, and sound pressure levels significantly impact vitality. A sound pressure level of 77 dB has been identified as a critical threshold in emotional perception models. Consequently, distinct emotional prediction models can be employed to enhance landscape design across various sound pressure level zones. This research provides scientific evidence and flexible strategies for designing urban open spaces that improve landscape experiences based on multisensory perception.
Spatio-Temporal Heterogeneity of the Urban Heat Effect and Its Socio-Ecological Drivers in Yangzhou City, China
Authors: Wu Tao , Wang Zhaoyi , Xu Qiang
Published: September 2024

Rapid urbanization and land-use changes may affect the intensity of urban heat islands (UHIs). However, research on the eastern Chinese city of Yangzhou is lacking. Using land cover data and the InVest Urban Cooling model, this study evaluated the spatiotemporal heterogeneity of the UHI effect from 1990 to 2020 and its socioecological drivers in Yangzhou City. Landscape pattern indices such as patch area (CA), percentage of landscape (PLAND), number of patches, patch density, and aggregation index were created using Fragstats 4.2 software. Several social indicators, such as gross domestic product (GDP), night-light index, and population density, were considered to explore their correlation with UHI indicators. During the past three decades, rapid urbanization in Yangzhou has intensified the UHI effect, with the cooling capacity (cc park) and heat mitigation index (HMI) decreasing by ~9.6%; however, the mixed air temperature (T air) has increased by 0.14 °C. The main heat island areas are concentrated in southern Yangzhou, including the Hanjiang and Guangling districts, and have expanded over time. T air was positively correlated with GDP, night-light index, and population density. Moreover, for the impervious land use type, cc park and HMI were negatively correlated with CA and PLAND (p < 0.01). This study contributes to a deeper understanding of the dynamics of UHIs and provides valuable insights for policymakers, urban planners, and researchers striving to create sustainable and climate-resilient cities in Yangzhou.
The Prevalence of Arrhythmias, Including Premature Supraventricular and Ventricular Beats and Other Electrocardiographic Patterns, in 24-Hour Holter Monitoring in Patients with Overweight and Obesity
Authors: Dykiert Irena Anna , Kraik Krzysztof , Jurczenko Lidia , Gać Paweł , Poręba Rafał , Poręba Małgorzata
Published: September 2024

Objectives: this study aims to evaluate the prevalence of various arrhythmias and other electrocardiographic patterns within the group of individuals with overweight and obesity. Methods: One hundred eighty-one adults (90 females and 91 males) were qualified for inclusion in the experimental group. All participants had a body mass index (BMI) exceeding 25 kg/m2 (98 patients with obesity and 83 with overweight). The mean BMI in the obesity group was 33.6 kg/m2, and all participants had class 1 obesity. The control group comprised 69 individuals (56 females and 13 males) with normal BMI. The basic measurements were performed, and the participants filled out questionnaires describing their health conditions and lifestyles. Each participant underwent an electrocardiographic (ECG) examination and a 24 h Holter ECG examination. Results: In patients with class 1 obesity compared to the control patients, the average numbers of premature ventricular beats (PVBs) and premature supraventricular beats (SPBs) were statistically significantly higher (p = 0.030 and p = 0.042). There was a positive correlation between body weight and PVB (p = 0.028) and between body weight and SPB (p = 0.028). Moreover, BMI and waist circumference were correlated with SPB (p = 0.043 and p = 0.031). In the backward stepwise multivariate regression model considering 24 h Holter ECG monitoring, concerning SPB as the dependent variable, it was observed that BMI (especially obesity class 1), type 2 diabetes, and thyroid disease exhibited the highest regression coefficients. Conclusions: obesity, even in class 1, might be a factor in a more frequent occurrence of abnormalities in electrocardiographic tests.