bluuebunny/crossref_metadata_2025 huggingface dataset:
Structural organization and sequence diversity of the complete nucleotide sequence encoding the Plasmodium malariae merozoite surface protein-1
Authors: Putaporntip Chaturong , Kuamsab Napaporn , Rojrung Rattanaporn , Seethamchai Sunee , Jongwutiwes Somchai
Published: September 2022

The merozoite surface protein-1 (MSP1) is a prime candidate for an asexual blood stage vaccine against malaria. However, polymorphism in this antigen could compromise the vaccine’s efficacy. Although the extent of sequence variation inMSP1has been analyzed from variousPlasmodiumspecies, little is known about structural organization and diversity of this locus inPlasmodium malariae(PmMSP1). Herein, we have shown thatPmMSP1contained five conserved and four variable blocks based on analysis of the complete coding sequences. Variable blocks were characterized by short insertion and deletion variants (block II), polymorphic nonrepeat sequences (block IV), complex repeat structure with size variation (block VI) and degenerate octapeptide repeats (block VIII). Like other malarialMSP1s, evidences of intragenic recombination have been found inPmMSP1. The rate of nonsynonymous nucleotide substitutions significantly exceeded that of synonymous nucleotide substitutions in block IV, suggesting positive selection in this region. Codon-based analysis of deviation from neutrality has identified a codon under purifying selection located in close proximity to the homologous region of the 38 kDa/42 kDa cleavage site ofP. falciparum MSP1. A number of predicted linear B-cell epitopes were identified across both conserved and variable blocks of the protein. However, polymorphism in repeat-containing blocks resulted in alteration of the predicted linear B-cell epitope scores across variants. Although a number of predicted HLA-class II-binding peptides were identified in PmMSP1, all variants of block IV seemed not to be recognized by common HLA-class II alleles among Thai population, suggesting that diversity in this positive selection region could probably affect host immune recognition. The data on structural diversity inPmMSP1could be useful for further studies such as vaccine development and strain characterization of this neglected malaria parasite.
Characteristics of urine spraying and scraping the ground with hind paws as scent-marking of captive cheetahs (Acinonyx jubatus)
Authors: Kinoshita Kodzue , Suzuki Misa , Sasaki Yuuta , Yonezawa Aya , Kamitani Hisayoshi , Okuda Ryuta , Ishikawa Tatsuya , Tsukui Kenta , Kohshima Shiro
Published: September 2022

Olfactory communication is common in felids. We observed two scent-markings, urine spraying and scraping the ground with hind paws during excretion, of 25 captive cheetahs. We analyzed the association of sniffing with the timing of urine spraying and scraping, and differences in these behaviors based on sex, age, and captive environment to understand the olfactory communication among cheetahs. Both scent-markings were strongly associated with sniffing, especially scraping, and the presence or absence of scent was thought to be a trigger. Both behaviors were observed only in adults; scraping was observed only in males. To our knowledge, this study was first to confirm the discharge of secretions from the anal glands during scraping. The frequencies of both behaviors were significantly higher in males kept in shared enclosures containing other individuals than in males kept in monopolized enclosures, while there was no difference in the frequencies among females. Female cheetahs are solitary and have non-exclusive home range, whereas male cheetahs are either solitary or live in coalition groups and there are territorial and non-territorial males. Our results could be attributed to the differences in sociality between the sexes and effect of the living environment.
Prediction of visual function from automatically quantified optical coherence tomography biomarkers in patients with geographic atrophy using machine learning
Authors: Balaskas Konstantinos , Glinton S. , Keenan T. D. L. , Faes L. , Liefers B. , Zhang G. , Pontikos N. , Struyven R. , Wagner S. K. , McKeown A. , Patel P. J. , Keane P. A. , Fu D. J.
Published: September 2022

Geographic atrophy (GA) is a vision-threatening manifestation of age-related macular degeneration (AMD), one of the leading causes of blindness globally. Objective, rapid, reliable, and scalable quantification of GA from optical coherence tomography (OCT) retinal scans is necessary for disease monitoring, prognostic research, and clinical endpoints for therapy development. Such automatically quantified biomarkers on OCT are likely to further elucidate structure–function correlation in GA and thus the pathophysiological mechanisms of disease development and progression. In this work, we aimed to predict visual function with machine-learning applied to automatically acquired quantitative imaging biomarkers in GA. A post-hoc analysis of data from a clinical trial and routine clinical care was conducted. A deep-learning automated segmentation model was applied on OCT scans from 476 eyes (325 patients) with GA. A separate machine learning prediction model (Random Forest) used the resultant quantitative OCT (qOCT) biomarkers to predict cross-sectional visual acuity under standard (VA) and low luminance (LLVA). The primary outcome was regression coefficient (r2) and mean absolute error (MAE) for cross-sectional VA and LLVA in Early Treatment Diabetic Retinopathy Study (ETDRS) letters. OCT parameters were predictive of VA (r2 0.40 MAE 11.7 ETDRS letters) and LLVA (r2 0.25 MAE 12.1). Normalised random forest feature importance, as a measure of the predictive value of the three constituent features of GA; retinal pigment epithelium (RPE)-loss, photoreceptor degeneration (PDR), hypertransmission and their locations, was reported both on voxel-level heatmaps and ETDRS-grid subfields. The foveal region (46.5%) and RPE-loss (31.1%) had greatest predictive importance for VA. For LLVA, however, non-foveal regions (74.5%) and PDR (38.9%) were most important. In conclusion, automated qOCT biomarkers demonstrate predictive significance for VA and LLVA in GA. LLVA is itself predictive of GA progression, implying that the predictive qOCT biomarkers provided by our model are also prognostic.
The interaction between LC8 and LCA5 reveals a novel oligomerization function of LC8 in the ciliary-centrosome system
Authors: Szaniszló Tamás , Fülöp Máté , Pajkos Mátyás , Erdős Gábor , Kovács Réka Ágnes , Vadászi Henrietta , Kardos József , Dosztányi Zsuzsanna
Published: September 2022

Dynein light chain LC8 is a small dimeric hub protein that recognizes its partners through short linear motifs and is commonly assumed to drive their dimerization. It has more than 100 known binding partners involved in a wide range of cellular processes. Recent large-scale interaction studies suggested that LC8 could also play a role in the ciliary/centrosome system. However, the cellular function of LC8 in this system remains elusive. In this work, we characterized the interaction of LC8 with the centrosomal protein lebercilin (LCA5), which is associated with a specific form of ciliopathy. We showed that LCA5 binds LC8 through two linear motifs. In contrast to the commonly accepted model, LCA5 forms dimers through extensive coiled coil formation in a LC8-independent manner. However, LC8 enhances the oligomerization ability of LCA5 that requires a finely balanced interplay of coiled coil segments and both binding motifs. Based on our results, we propose that LC8 acts as an oligomerization engine that is responsible for the higher order oligomer formation of LCA5. As LCA5 shares several common features with other centrosomal proteins, the presented LC8 driven oligomerization could be widespread among centrosomal proteins, highlighting an important novel cellular function of LC8.
Identification and characterization of the kynurenine pathway in the pond snail Lymnaea stagnalis
Authors: Cristina Benatti , Veronica Rivi , Silvia Alboni , Andrea Grilli , Sara Castellano , Luca Pani , Nicoletta Brunello , M.C. Blom Johanna , Silvio Bicciato , Fabio Tascedda
Published: September 2022

Dysregulation of the kynurenine pathway (KP) is implicated in many human diseases and disorders, from immunological, metabolic, neurodegenerative, and neuropsychiatric conditions to cancer, and represents an appealing target for new therapeutic approaches. In this intricate scenario, invertebrates,like Lymnaea stagnalis(LS), provide a flexible tool to unravel the complexity of the KP. Starting from the availableLSgenome and transcriptome, we identified putative transcripts of all KP enzymes containing an ORF; each predicted protein possessed a high degree of sequence conservation to known orthologues of other invertebrate and vertebrate model organisms. Sequences were confirmed by qualitative PCR and sequencing. At the same time, the qRT-PCR analysis revealed that Lym IDO-like, Lym TDO-like, Lym AFMID-like, Lym KMO-like, Lym AADAT-like, Lym KYAT I/III-like, Lym KYNU-like, Lym HAAO-like, and Lym ACMSD-like showed widespread tissue expression. Then, tryptophan, kynurenine, kynurenic acid, anthranilic acid, 3-hydroxy-kynurenine, xanthurenic acid, picolinic acid, and quinolinic acid were identified in the hemolymph of LS by UHPLC-Q exactive mass spectrometer. Our study provides the most thorough characterization to date of the KP in an invertebrate model, supporting the value of LS for future functional studies of this pathway at the cellular, synaptic, and behavioral levels.
On a two-dimensional model of generalized thermoelasticity with application
Authors: Ahmed Ethar A. A. , El-Dhaba A. R. , Abou-Dina M. S. , Ghaleb A. F.
Published: September 2022

A 2D first order linear system of partial differential equations of plane strain thermoelasticity within the frame of extended thermodynamics is presented and analyzed. The system is composed of the equations of classical thermoelasticity in which displacements are replaced with velocities, complemented with Cattaneo evolution equation for heat flux. For a particular choice of the characteristic quantities and for positive thermal conductivity, it is shown that this system may be cast in a form that is symmetric t-hyperbolic without further recurrence to entropy principle. While hyperbolicity means a finite speed of propagation of heat waves, it is known that symmetric hyperbolic systems have the desirable property of well-posedness of Cauchy problems. A study of the characteristics of this system is carried out, and an energy integral is derived, that can be used to prove uniqueness of solution under some boundary conditions. A numerical application for a finite slab is considered and the numerical results are plotted and discussed. In particular, the wave propagation nature of the solution is put in evidence.
The function of a conidia specific transcription factor CsgA in Aspergillus nidulans
Authors: Cho He-Jin , Park Hee-Soo
Published: September 2022

Aspergillus spp. mainly reproduce asexually via asexual spores called conidia. In this study, we identified CsgA, a conidia-specific Zn2Cys6 transcription factor containing the GAL4-like zinc-finger domain, and characterized the roles of CsgA in the model organism Aspergillus nidulans. In A. nidulans, the ΔcsgA strain produced abnormal conidiophores and exhibited increased conidial production. The deletion of csgA resulted in impaired production of sexual fruiting bodies (cleistothecia) and lower mutA expression levels. Overexpression of csgA led to decreased conidia production but increased cleistothecia production, suggesting that CsgA is essential for proper asexual and sexual development in A. nidulans. In conidia, the deletion of csgA resulted in increased trehalose content, higher spore viability, and increased tolerance to thermal and oxidative stresses. Transcriptomic analysis revealed that the loss of csgA affects the expression of genes related to conidia germination, DNA repair, and secondary metabolite biosynthesis. Further analysis revealed that the ΔcsgA strain exhibited delayed conidial germination and abnormal germ tube length. Additionally, the production of sterigmatocystin increased in the ΔcsgA conidia compared to that in the controls. Overall, these results suggest that CsgA is crucial for proper fungal development, spore viability, conidial germination, and sterigmatocystin production in A. nidulans.
Short-term paleogeographic reorganizations and climate events shaped diversification of North American freshwater gastropods over deep time
Authors: Neubauer Thomas A. , Harzhauser Mathias , Hartman Joseph H. , Silvestro Daniele , Scotese Christopher R. , Czaja Alexander , Vermeij Geerat J. , Wilke Thomas
Published: September 2022

What controls species diversity and diversification is one of the major questions in evolutionary biology and paleontology. Previous studies have addressed this issue based on various plant and animal groups, geographic regions, and time intervals. However, as most previous research focused on terrestrial or marine ecosystems, our understanding of the controls on diversification of biota (and particularly invertebrates) in freshwater environments in deep time is still limited. Here, we infer diversification rates of North American freshwater gastropods from the Late Triassic to the Pleistocene and explore potential links between shifts in speciation and extinction and major changes in paleogeography, climate, and biotic interactions. We found that variation in the speciation rate is best explained by changes in continental fragmentation, with rate shifts coinciding with major paleogeographic reorganizations in the Mesozoic, in particular the retreat of the Sundance Sea and subsequent development of the Bighorn wetland and the advance of the Western Interior Seaway. Climatic events in the Cenozoic (Middle Eocene Climate Optimum, Miocene Climate Optimum) variably coincide with shifts in speciation and extinction as well, but no significant long-term association could be detected. Similarly, no influence of diversity dependence was found across the entire time frame of ~ 214 Myr. Our results indicate that short-term climatic events and paleogeographic changes are relevant to the diversification of continental freshwater biota, while long-term trends have limited effect.
Bayesian inference of spectrometric data and validation with numerical simulations of plasma sheath diagnostics of a plasma focus discharge
Authors: Avaria Gonzalo , Clausse Alejandro , Davis Sergio , Pavez Cristian , Villalba Nelson , Cuadrado Osvaldo , Moreno Jose , Ruiz H. Marcelo , Soto Leopoldo
Published: September 2022

Plasma Foci are pulsed coaxial discharges with numerous radiation applications and interesting scientific phenomena. Although the physics answered much of the processes involved in these discharges, many related fundamental questions still remains doggedly unresolved. One of the obstacles to deeper knowledge is the scarcity of reliable experimental data. This work presents an elaborate experimental assessment of the electron density in the rundown phase of a 400 J Plasma Focus operating with hydrogen. The rundown of the plasma sheath is basically a hypersonic shock wave between two coaxial electrodes accelerated by the Lorentz force, and it is important to control the pinch formation. The electron density of the passing sheath is measured by means of the Stark broadened hydrogen alpha emission with spatial and temporal resolution. The experimental data is post-processed using Bayesian posterior probability assessment. The results are conflated with the numerical model CShock to construe an educated explanation of the sheath behavior during the rundown. In particular, it is possible to reckon the formation of a toroidal instability reported in previous experiments, and to estimate the plasma sheath temperature (4–20 eV) and velocity (62.5 km/s) at this stage.
Impact of nutrition and physical activity on outcomes of hospital-acquired pneumonia
Authors: Jang Jin Ho , Kim Taehwa , Yeo Hye Ju , Cho Woo Hyun , Min Kyung Hoon , Oh Jee Youn , Hong Sang-Bum , Baek Ae-Rin , Lee Hyun-Kyung , Kim Changhwan , Chang Youjin , Park Hye Kyeong , Lee Heung Bum , Bae Soohyun , Moon Jae Young , Yoo Kwang Ha , Gil Hyun-Il , Shin Beomsu , Jeon Kyeongman ,   the Korean HAP/VAP Study Group, Cho Woo Hyun , Min Kyung Hoon , Oh Jee Youn , Hong Sang-Bum , Baek Ae-Rin , Lee Hyun-Kyung , Kim Changhwan , Chang Youjin , Park Hye Kyeong , Lee Heung Bum , Bae Soohyun , Moon Jae Young , Yoo Kwang Ha , Gil Hyun-Il , Shin Beomsu , Jeon Kyeongman
Published: September 2022

Frailty is an important risk factor for adverse health-related outcomes. It is classified into several phenotypes according to nutritional state and physical activity. In this context, we investigated whether frailty phenotypes were related to clinical outcome of hospital-acquired pneumonia (HAP). During the study period, a total of 526 patients were screened for HAP and 480 of whom were analyzed. The patients were divided into four groups according to physical inactivity and malnutrition: nutritional frailty (Geriatric Nutritional Risk Index [GNRI] < 82 and Clinical Frailty Scale [CFS] ≥ 4), malnutrition (GNRI < 82 and CFS < 4), physical frailty (GNRI ≥ 82 and CFS ≥ 4), and normal (GNRI ≥ 82 and CFS < 4). Among the phenotypes, physical frailty without malnutrition was the most common (39.4%), followed by nutritional frailty (30.2%), normal (20.6%), and malnutrition (9.8%). There was a significant difference in hospital survival and home discharge among the four phenotypes (p = 0.009), and the nutritional frailty group had the poorest in-hospital survival and home discharge (64.8% and 34.6%, respectively). In conclusion, there were differences in clinical outcomes according to the four phenotypes of HAP. Assessment of frailty phenotypes during hospitalization may improve outcomes through adequate nutrition and rehabilitation treatment of patients with HAP.
Production of cold-active pectinases by three novel Cladosporium species isolated from Egypt and application of the most active enzyme
Authors: Moharram Ahmad Mohamed , Zohri Abdel-Naser Ahmed , Hesham Abd El-Latif , Abdel-Raheam Hossam E. F. , Al-Ameen Maher Mohamed , Al-Bedak Osama Abdel-Hafeez
Published: September 2022

Cladosporium parasphaerospermum,Cladosporium chlamydosporigenum, andCladosporium compactisporumhave all been discovered and characterized as newCladosporiumspecies. The three new species seemed to generate cold-active pectinases with high activity at pH 6.0 and 10 °C, pH 6.0 and 15 °C, and pH 5.0 and 15 °C, respectively, with the most active beingC. parasphaerospermumpectinase. In submerged fermentation (SmF),C. parasphaerospermumproduced the most cold-active pectinase with the highest activity and specific activity (28.84 U/mL and 3797 U/mg) after 8 days.C. parasphaerospermumcold-active pectinase was isolated using DEAE-Cellulose anion exchange resin and a Sephadex G 100 gel filtration column. The enzyme was purified 214.4-fold and 406.4-fold greater than the fermentation medium using DEAE-cellulose and Sephadex G 100, respectively. At pH 7.0 and 10 °C, pure pectinase had the highest activity (6684 U/mg), with Kmand Vmaxdetermined to be 26.625 mg/mL and 312.5 U/min, respectively. At 5 mM/mL, EDTA, MgCl2, and SDS inhibited the activity of pure pectinase by 99.21, 96.03, and 94.45%, respectively. The addition of 10 U/mL pure pectinase enhanced the yield of apple, orange, apricot, and peach juice by 17, 20, 13, and 24%, respectively, and improved the clarity and colour of orange juice by 194 and 339%, respectively. We can now add cold-active pectinase production to the long list ofCladosporiumspecies that have been identified. We also report three new species that can be used in biotechnological solutions as active microbial pectinase producers. Although further research is needed, these distinct species might be used to decompose difficult and resistant pectinacious wastes as well as clear fruit juices.
Differences in spinal posture and mobility between children/adolescents with obesity and age-matched normal-weight individuals
Authors: Bayartai M. E. , Schaer C. E. , Luomajoki Hannu , Tringali G. , De Micheli R. , Sartorio A.
Published: September 2022

The aim of this study was to cross-sectionally explore the association of obesity with spinal posture and mobility, commonly associated with musculoskeletal problems, by comparing the spinal parameters between 90 obese and 109 normal-weight children and adolescents. A non-invasive electromechanical device, the Idiag M360 (Idiag, Fehraltorf, Switzerland), was used to measure the spinal parameters. An age-and-sex-adjusted two-way analysis of variance (ANOVA) was used to determine postural and mobility differences between the two groups. Children and adolescents with obesity had significantly greater thoracic kyphosis [difference between groups (Δ) = 13.00, 95% CI 10.10–15.80, p < 0.0001] and thoracic extension (Δ = 6.50, 95% CI 2.90–11.60, p = 0.005), as well as smaller mobility in thoracic flexion (Δ = 5.00, 95% CI 1.20–8.80, p = 0.01), thoracic lateral flexion (Δ = 17.70, 95% CI 11.60–23.80, p < 0.0001), lumbar flexion (Δ = 12.10, 95% CI 8.70–15.50, p < 0.0001), lumbar extension (Δ = 7.10, 95% CI 3.10–12.20, p = 0.003) and lumbar lateral flexion (Δ = 9.10, 95% CI 5.50–12.80, p < 0.0001) compared to the normal-weight children and adolescents. These findings provide important information about the characteristics of the spine in children and adolescents with obesity and unique insights into obesity-related mechanical challenges that the spine has to withstand and strategies designed to improve spinal mobility in this young population.
Walking paths during collaborative carriages do not follow the simple rules observed in the locomotion of single walking subjects
Authors: Maroger Isabelle , Silva Manon , Pillet Hélène , Turpin Nicolas , Stasse Olivier , Watier Bruno
Published: September 2022

Some works have already studied human trajectories during spontaneous locomotion. However, this topic has not been thoroughly studied in the context of human-human interactions, especially during collaborative carriage tasks. Thus, this manuscript aims to provide a broad analysis of the kinematics of two subjects carrying a table. In the present study, 20 pairs of subjects moved a table to 9 different goal positions distant of 2.7–5.4 m. This was performed with only one or both subjects knowing the target location. The analysis of the collected data demonstrated that there is no shared strategy implemented by all the pairs to move the table around. We observed a great variability in the pairs’ behaviours. Even the same pair can implement various strategies to move a table to the same goal position. Moreover, a model of the trajectories adopted by collaborating pairs was proposed and optimized with an inverse optimal control scheme. Even if it produced consistent results, due to the great variability which origins were not elucidated, it was not possible to accurately simulate the average trajectories nor the individual ones. Thus, the approach that was shown to be efficient to simulate single walking subjects failed to model the behaviour of collaborating pairs.
Association of socioeconomic deprivation with life expectancy and all-cause mortality in Spain, 2011–2013
Authors: Redondo-Sánchez Daniel , Sánchez María-José , Fernández-Navarro Pablo , Rachet Bernard , Luque-Fernandez Miguel Angel
Published: September 2022

Life tables summarise a population’s mortality experience during a time period. Sex- and age-specific life tables are needed to compute various cancer survival measures. However, mortality rates vary according to socioeconomic status. We present sex- and age-specific life tables based on socioeconomic status at the census tract level in Spain during 2011–2013 that will allow estimating cancer relative survival estimates and life expectancy measures by socioeconomic status. Population and mortality data were obtained from the Spanish Statistical Office. Socioeconomic level was measured using the Spanish Deprivation Index by census tract. We produced sex- and age-specific life expectancies at birth by quintiles of deprivation, and life tables by census tract and province. Life expectancy at birth was higher among women than among men. Women and men in the most deprived census tracts in Spain lived 3.2 and 3.8 years less than their counterparts in the least deprived areas. A higher life expectancy in the northern regions of Spain was discovered. Life expectancy was higher in provincial capitals than in rural areas. We found a significant life expectancy gap and geographical variation by sex and socioeconomic status in Spain. The gap was more pronounced among men than among women. Understanding the association between life expectancy and socioeconomic status could help in developing appropriate public health programs. Furthermore, the life tables we produced are needed to estimate cancer specific survival measures by socioeconomic status. Therefore, they are important for cancer control in Spain.
Continued weakening of the equatorial Pacific upwelling annual cycle in CMIP5 future projections
Authors: Wang Li-Chiao , Dao Thi Lan , Yu Jia-Yuh
Published: September 2022

This study explores the dynamics of the equatorial Pacific upwelling annual cycle under global warming using the Coupled Model Intercomparison Project Phase 5 (CMIP5) simulations. Through a linear-weighted theory developed recently, the theoretical upwelling annual cycles under global warming helped reasonably characterize the patterns of the original upwelling annual cycles simulated in CMIP5 models; however, an apparent weakening in magnitude as compared to that during the present stage was observed. To verify the above, we divided 90-year outputs in the CMIP5 future projections into three 30-year windows and set side by side. The long-term evolution of the upwelling annual cycle reconfirmed an overall weakening tendency in the entire equatorial Pacific. Moreover, the weakening of the Ekman upwelling could most likely be attributed to the meridional surface wind stress divergence, while the gradually smoothing inclination in the overall equatorial thermocline depth was responsible for the weakening of the wave upwelling. The weakening of the wave upwelling in the east and the Ekman upwelling in the west jointly contributed to the gradual weakening of the equatorial Pacific upwelling annual cycle. The above projected changes are robust among the 19 chosen CMIP5 models. Equatorial upwelling largely influences the sea surface temperature, associated atmosphere–ocean interactions, and convection and precipitation in tropical areas; hence, a continuous weakening of the upwelling annual cycle over the equatorial Pacific Ocean could likely affect the major climate phenomena variability with strong seasonal-locking characteristics by modifying the background strength at their peak phases in the future. The theoretical results can provide us the equatorial upwelling annual cycle patterns based on the Ekman and wave dynamics, which would be a strong tool for our investigations on the climate variability under global warming.
The influence of sexual activity on athletic performance: a systematic review and meta-analyses
Authors: Zavorsky Gerald S. , Brooks Rebecca A.
Published: September 2022

Several anecdotal reports suggest that sex before competition can affect performance. Our objective was to perform a systematic review and meta-analysis to determine whether athletic performance or some physical fitness measure is affected by prior sexual activity. Web of Science (all databases) and Google Scholar were used to identify studies from which adult healthy subjects were included. As all studies were crossover trials, an inverse variance statistical method with random effects was used to minimize the uncertainty of the pooled effect estimate. Bias was assessed via the revised Cochrane Risk of Bias tool (RoB 2) with a "per protocol" analysis. Nine crossover studies (133 subjects, 99% male) were used in this meta-analysis. All those studies did not examine athletic performance per se, but all studies assessed one or more physical fitness parameters. The RoB 2 suggested that overall, there were some concerns with bias. As there was moderate heterogeneity amongst the different outcomes (Tau2 = 0.02, Chi-square = 17.2, df = 8, p = 0.03, I2 = 54%), a random-effects model was used. The results neither favored abstinence nor sexual activity before a physical fitness test [standardized mean difference = 0.03 (− 0.10 to 0.16), Z = 0.47, p = 0.64, where a negative standardized mean difference favors abstinence, and a positive standardized mean difference favors sexual activity]. The results demonstrate that sexual activity within 30 min to 24 h before exercise does not appear to affect aerobic fitness, musculoskeletal endurance, or strength/power.
Poor glycemic control and associated factors among pediatric diabetes mellitus patients in northwest Ethiopia, 2020: facility-based cross sectional retrospective study design
Authors: Kidie Atitegeb Abera , Ayal Birtukan Gizachew , Ayele Tiruneh , Fentie Elsa Awoke , Lakew Ayenew Molla
Published: September 2022

Diabetes mellitus is a global public health problem. Glycemic control is a major public health problem. Diabetes results from elevated levels of glycaemia such as increased glucose and glycated hemoglobin, and controlling glycaemia is an integral component of the management of diabetes. Glycemic control in children is particularly difficult to achieve. Identifying determinants of poor glycemic control is important for early modification of diabetic related end organ damages. This study was aimed to assess the status of glycemic control and associated factors among pediatric diabetes mellitus patients in northwest Ethiopia. Facility-based cross sectional retrospective cohort study design was used and this study was conducted from September, 2015 to February, 2018. Simple random sampling was used to select 389 samples. Data were collected using an extraction checklist. Data were entered into Epi-data − 4.6, and analyzed using Stata-16. Finally, multivariable binary logistic regression was done. Poor glycemic control was more common among pediatric patients 39.3% (95% CI 34.6, 44.3). Treatment discontinuation (AOR 2.42, 95% CI 1.25, 4.69), age (AOR 1.15, 95% CI 1.03, 1.28) and treatment dose (AOR 0.96, 95 CI 0.92, 0.99) were significantly associated with poor glycemic control. Prevalence of poor glycemic control was high. Patient’s age, history of treatment discontinuation and dose of treatment were the significant contributing factors to poor glycemic control. These need to be addressed to attain the objective of adequate glycemic control.
Bryozoan–cnidarian mutualism triggered a new strategy for greater resource exploitation as early as the Late Silurian
Authors: Zapalski Mikołaj K. , Vinn Olev , Toom Ursula , Ernst Andrej , Wilson Mark A.
Published: September 2022

Bryozoans were common benthic invertebrates in the Silurian seas. The large biodiversity among Silurian benthic organisms prompted diversified interactions, and as a result bryozoans hosted many other organisms as symbionts. Here we analyse the cystoporate bryozoanFistulipora przhidolensisand unidentified trepostomes intergrown with auloporid tabulate corals and putative hydrozoans. The material comes from the uppermost Přídolí Series (Late Silurian) of the Sõrve Peninsula, Saaremaa, Estonia. Our analysis shows that the interaction was beneficial for both organisms—cnidarians benefited from feeding currents created by the host bryozoan, while the latter benefited from the protection from predators by cnidae, it can thus be classified as mutualism. Such associations are common in modern seas. The analysed organisms are typically encrusting when the symbiosis is absent, when intergrown they display erect, branching morphologies, raised over the substratum, thus exploiting a higher suspension-feeding tier. While similar associations were known from the Devonian, we demonstrate that this novel ecological strategy for greater resource exploitation started as early as the latest Silurian.
Reevaluation of antibody-dependent enhancement of infection in anti-SARS-CoV-2 therapeutic antibodies and mRNA-vaccine antisera using FcR- and ACE2-positive cells
Authors: Shimizu Jun , Sasaki Tadahiro , Koketsu Ritsuko , Morita Ryo , Yoshimura Yuka , Murakami Ami , Saito Yua , Kusunoki Toshie , Samune Yoshihiro , Nakayama Emi E. , Miyazaki Kazuo , Shioda Tatsuo
Published: September 2022

Many therapeutic antibodies (Abs) and mRNA vaccines, both targeting SARS-CoV-2 spike protein (S-protein), have been developed and approved in order to combat the ongoing COVID-19 pandemic. In consideration of these developments, a common concern has been the potential for Ab-dependent enhancement (ADE) of infection caused by inoculated or induced Abs. Although the preventive and therapeutic effects of these Abs are obvious, little attention has been paid to the influence of the remaining and dwindling anti-S-protein Abs in vivo. Here, we demonstrate that certain monoclonal Abs (mAbs) approved as therapeutic neutralizing anti-S-protein mAbs for human usage have the potential to cause ADE in a narrow range of Ab concentrations. Although sera collected from mRNA-vaccinated individuals exhibited neutralizing activity, some sera gradually exhibited dominance of ADE activity in a time-dependent manner. None of the sera examined exhibited neutralizing activity against infection with the Omicron strain. Rather, some ADE of Omicron infection was observed in some sera. These results suggest the possible emergence of adverse effects caused by these Abs in addition to the therapeutic or preventive effect.
Potential protective effects of chrysin against immunotoxicity induced by diazinon
Authors: Zeinali Majid , Shafaei Azam , Rafatpanah Houshang , Mosavat Arman , Tayebi-Meybodi Naser , Hosseinzadeh Hossein , Rezaee Seyed Abdolrahim
Published: September 2022

Acute intoxication with diazinon (DZN) as a pesticide causes mortality and morbidity annually. This study shows the impact of sub-acute toxicity of DZN 20 mg/kg and the protective activities of chrysin (CH) as a flavone under the flavonoids family (12.5, 25 and 50 mg/kg) were assessed on BALB/c mouse immune system. The changes in morphological and functional properties of the immune system on thymus, spleen and liver histopathology, sub-populations of T lymphocytes, cytokines levels, transcription factors, complement function, phagocytosis, specific and total antibody productions were considered. The histopathological effects of DZN on the spleen and thymus were not significant, but the liver was damaged remarkably. In the presence of CH, the toxic effect of DZN is suppressed. DZN significantly decreased the number of whole blood TCD4+, TCD8+ and NK cells and suppressed the phagocytosis, delayed-type hypersensitivity (DTH) responses to sheep red blood cell (SRBC). Furthermore, it suppressed specific anti-SRBC-Ab, total IgG and IgM production, T-bet expression, and IFN-γ production. In contrast, DZN did not significantly affect complement function and the number of NK cells, TCD4+ and TCD8+ splenocytes. However, it potentiated the expression of GATA-3, ROR-γt and FOXP3 gene expression and consequently produced IL-4, IL-10, IL-17 and TGF-β in whole blood. CH not only significantly increased the variables mentioned above at 12.5, 25 and 50 mg/kg but also could overcome the toxic effects of DZN on whole blood lymphocyte sub-populations and specific and total Ab production in 25 and 50 mg/kg concentrations, phagocytosis and DTH responses in 50 mg/kg, and modulation of the transcription factors and cytokine production, mainly in 25 and 50 mg/kg. In conclusion, DZN in sub-acute doses could remarkably deteriorate immune responses. However, CH can overcome the toxic effects of DZN on the immune components and functions of the immune system.
Smartphone camera oximetry in an induced hypoxemia study
Authors: Hoffman Jason S. , Viswanath Varun K. , Tian Caiwei , Ding Xinyi , Thompson Matthew J. , Larson Eric C. , Patel Shwetak N. , Wang Edward J.
Published: September 2022

Hypoxemia, a medical condition that occurs when the blood is not carrying enough oxygen to adequately supply the tissues, is a leading indicator for dangerous complications of respiratory diseases like asthma, COPD, and COVID-19. While purpose-built pulse oximeters can provide accurate blood-oxygen saturation (SpO2) readings that allow for diagnosis of hypoxemia, enabling this capability in unmodified smartphone cameras via a software update could give more people access to important information about their health. Towards this goal, we performed the first clinical development validation on a smartphone camera-based SpO2 sensing system using a varied fraction of inspired oxygen (FiO2) protocol, creating a clinically relevant validation dataset for solely smartphone-based contact PPG methods on a wider range of SpO2 values (70–100%) than prior studies (85–100%). We built a deep learning model using this data to demonstrate an overall MAE = 5.00% SpO2 while identifying positive cases of low SpO2 < 90% with 81% sensitivity and 79% specificity. We also provide the data in open-source format, so that others may build on this work.
Hidden paths to endless forms most wonderful: ecology latently shapes evolution of multicellular development in predatory bacteria
Authors: La Fortezza Marco , Rendueles Olaya , Keller Heike , Velicer Gregory J.
Published: September 2022

Ecological causes of developmental evolution, for example from predation, remain much investigated, but the potential importance of latent phenotypes in eco-evo-devo has received little attention. Using the predatory bacteriumMyxococcus xanthus, which undergoes aggregative fruiting body development upon starvation, we tested whether adaptation to distinct growth environments that do not induce development latently alters developmental phenotypes under starvation conditions that do induce development. In an evolution experiment named MyxoEE-3, growingM. xanthuspopulations swarmed across agar surfaces while adapting to conditions varying at factors such as surface stiffness or prey identity. Such ecological variation during growth was found to greatly impact the latent evolution of development, including fruiting body morphology, the degree of morphological trait correlation, reaction norms, degrees of developmental plasticity and stochastic diversification. For example, some prey environments promoted retention of developmental proficiency whereas others led to its systematic loss. Our results have implications for understanding evolutionary interactions among predation, development and motility in myxobacterial life cycles, and, more broadly, how ecology can profoundly shape the evolution of developmental systems latently rather than by direct selection on developmental features.
Artificial intelligence-driven design of fuel mixtures
Authors: Kuzhagaliyeva Nursulu , Horváth Samuel , Williams John , Nicolle Andre , Sarathy S. Mani
Published: September 2022

High-performance fuel design is imperative to achieve cleaner burning and high-efficiency engine systems. We introduce a data-driven artificial intelligence (AI) framework to design liquid fuels exhibiting tailor-made properties for combustion engine applications to improve efficiency and lower carbon emissions. The fuel design approach is a constrained optimization task integrating two parts: (i) a deep learning (DL) model to predict the properties of pure components and mixtures and (ii) search algorithms to efficiently navigate in the chemical space. Our approach presents the mixture-hidden vector as a linear combination of each single component’s vectors in each blend and incorporates it into the network architecture (the mixing operator (MO)). We demonstrate that the DL model exhibits similar accuracy as competing computational techniques in predicting the properties for pure components, while the search tool can generate multiple candidate fuel mixtures. The integrated framework was evaluated to showcase the design of high-octane and low-sooting tendency fuel that is subject to gasoline specification constraints. This AI fuel design methodology enables rapidly developing fuel formulations to optimize engine efficiency and lower emissions.
Projected topological branes
Authors: Panigrahi Archisman , Juričić Vladimir , Roy Bitan
Published: September 2022

Nature harbors crystals of dimensionality (d) only up to three. Here we introduce the notion of projected topological branes (PTBs): Lower-dimensional branes embedded in higher-dimensional parent topological crystals, constructed via a geometric cut-and-project procedure on the Hilbert space of the parent lattice Hamiltonian. When such a brane is inclined at a rational or an irrational slope, either a new lattice periodicity or a quasicrystal emerges. The latter gives birth to topoquasicrystals within the landscape of PTBs. As such PTBs are shown to inherit the hallmarks, such as the bulk-boundary and bulk-dislocation correspondences, and topological invariant, of the parent topological crystals. We exemplify these outcomes by focusing on two-dimensional parent Chern insulators, leaving its signatures on projected one-dimensional (1D) topological branes in terms of localized endpoint modes, dislocation modes and the local Chern number. Finally, by stacking 1D projected Chern insulators, we showcase the imprints of three-dimensional Weyl semimetals in d = 2, namely the Fermi arc surface states and bulk chiral zeroth Landau level, responsible for the chiral anomaly. Altogether, the proposed PTBs open a realistic avenue to harness higher-dimensional (d > 3) topological phases in laboratory.
Global food systems transitions have enabled affordable diets but had less favourable outcomes for nutrition, environmental health, inclusion and equity
Authors: Ambikapathi Ramya , Schneider Kate R. , Davis Benjamin , Herrero Mario , Winters Paul , Fanzo Jessica C.
Published: September 2022

Over the past 50 years, food systems worldwide have shifted from predominantly rural to industrialized and consolidated systems, with impacts on diets, nutrition and health, livelihoods, and environmental sustainability. We explore the potential for sustainable and equitable food system transformation (ideal state of change) by comparing countries at different stages of food system transition (changes) using food system typologies. Historically, incomes have risen faster than food prices as countries have industrialized, enabling a simultaneous increase in the supply and affordability of many nutritious foods. These shifts are illustrated across five food system typologies, from rural and traditional to industrial and consolidated. Evolving rural economies, urbanization and changes in food value chains have accompanied these transitions, leading to changes in land distribution, a smaller share of agri-food system workers in the economy and changes in diets. We show that the affordability of a recommended diet has improved over time, but food systems of all types are falling short of delivering optimal nutrition and health outcomes, environmental sustainability, and inclusion and equity for all. Six ‘outlier’ case studies (Tajikistan, Egypt, Albania, Ecuador, Bolivia and the United States of America) illustrate broad trends, trade-offs and deviations. With the integrated view afforded by typologies, we consider how sustainable transitions can be achieved going forward.
Declining severe fire activity on managed lands in Equatorial Asia
Authors: Sloan Sean , Locatelli Bruno , Andela Niels , Cattau Megan E. , Gaveau David , Tacconi Luca
Published: September 2022

Fire activity is declining globally due to intensifying land management, but trends remain uncertain for the humid tropics, particularly Equatorial Asia. Here, we report that rates of fire events deemed severe (≥75th severity percentile of 2002-2019) and very severe (≥90th percentile) for Indonesia declined 19-27% and 23-34% over 2002-2019, respectively, controlling for precipitation, where fire-event severity is given by total fire radiative power and duration. The severity of seasonal fire activity – a measure of extremeness – declined 16% in Sumatra and moderately elsewhere. Declines concentrated over mosaic croplands and nearby forest, accounting for one-fifth and one-quarter of fire activity, respectively, with each class contracting 11% amongst severe fire events. Declines were limited over mosaic lands with relatively limited cropping, despite accounting for a similar extent and one-fifth share of fire activity. Declines had an uncertain association with agricultural development but seemingly reflect related political and economic forces for economic and environmental security.
Tailored pathways toward revived farmland biodiversity can inspire agroecological action and policy to transform agriculture
Authors: Sietz Diana , Klimek Sebastian , Dauber Jens
Published: September 2022

Advances in agrochemistry in the 19th century, along with increased specialisation and intensification of food production, transformed agriculture triggering a farmland biodiversity crisis. Present economic incentives reinforce this crisis to an unprecedented scale. As the loss of farmland biodiversity undermines the basis of agroecosystems’ productivity and, hence, the sustainability of food systems, another transformation is urgently needed. Here, we advocate a concept of future pathways tailored to the characteristics of agricultural land systems and relate these to targeted farming approaches using agroecological principles. The concept depicts a transformative vision to effectively re-establish farmland biodiversity, a cornerstone of sustainable agriculture. It has the potential to support a systematic refinement of existing biodiversity and agricultural policies to enhance their impact and benefit for people and nature.
Non-conservative nature of boron in Arctic marginal ice zones
Authors: Vlahos Penny , Lee Kitack , Lee Chang-Ho , Barrett Lauren , Juranek Lauren
Published: September 2022

The Arctic Ocean is experiencing a net loss of sea ice. Ice-free Septembers are predicted by 2050 with intensified seasonal melt and freshening. Accurate carbon dioxide uptake estimates rely on meticulous assessments of carbonate parameters including total alkalinity. The third largest contributor to oceanic alkalinity is boron (as borate ions). Boron has been shown to be conservative in open ocean systems, and the boron to salinity ratio (boron/salinity) is therefore used to account for boron alkalinity in lieu of in situ boron measurements. Here we report this ratio in the marginal ice zone of the Bering and Chukchi seas during late spring of 2021. We find considerable variation in born/salinity values in ice cores and brine, representing either excesses or deficits of boron relative to salinity. This variability should be considered when accounting for borate contributions to total alkalinity (up to 10 µmol kg−1) in low salinity melt regions.