bluuebunny/crossref_metadata_2025 huggingface dataset:
Degenerative Changes in Aging Human Pelvic Lymph Nodes—A Reason to Rethink Staging and Therapy of Regional Malignancies?
Authors: Gödde Daniel , Degener Stephan , Walles Christine , Keller Rosalie , Graf Katharina , Tosch Marco , Krege Susanne , Musch Michael , Kvasnicka Hans Michael , Ackermann Maximilian , Störkel Stephan , von Rundstedt Friedrich-Carl
Published: September 2023

Lymph node metastases are common in pelvic urological tumors, and the age-related remodeling process of the pelvic lymph nodes influences metastatic behavior. The aim of this work is to characterize age-related degenerative changes in the pelvic lymph nodes with respect to their occurrence and extent. A total of 5173 pelvic lymph nodes of 390 patients aged 44 to 79 years (median 68 years, IQR 62–71 years) were histologically examined for degenerative structural changes. Lymph node size, lipomatous atrophy, capsular fibrosis, framework fibrosis, and calcifications were recorded semi-quantitatively and evaluated by age group. Significantly more lymph nodes <10 mm were found in older patients (p = 0.001). The incidence of framework fibrosis, capsular fibrosis, and calcifications increased significantly with increasing patient age (p < 0.001). In lipomatous atrophy, an increase in mild to moderate lipomatous atrophy was observed with increasing age (p < 0.001). In this, the largest study to date on this topic, age-related degenerative changes in pelvic lymph nodes were proven. Due to the consecutive decrease in hte filtration function of pelvic lymph nodes with increasing age, staging and therapy of metastatic pelvic urologic carcinomas should be reconsidered.
Tips for Preparing and Practicing Thermal Ablation Therapy of Hepatocellular Carcinoma
Authors: Minami Yasunori , Aoki Tomoko , Hagiwara Satoru , Kudo Masatoshi
Published: September 2023

Thermal ablation therapy, including radiofrequency ablation (RFA) and microwave ablation (MWA), is considered the optimal locoregional treatment for unresectable early-stage hepatocellular carcinomas (HCCs). Percutaneous image-guided ablation is a minimally invasive treatment that is being increasingly performed because it achieves good clinical outcomes with a lower risk of complications. However, the physics and principles of RFA and MWA markedly differ. Although percutaneous thermal ablation under image guidance may be challenging in HCC cases with limited access or a risk of thermal injury, a number of ablative techniques, each of which may be advantageous and disadvantageous for individual cases, are available. Furthermore, even when a HCC is eligible for ablation based on tumor selection and technical factors, additional patient factors may have an impact on whether it is the appropriate treatment choice. Therefore, a basic understanding of the advantages and limitations of each ablation device and imaging guidance technique, respectively, is important. We herein provide an overview of the basic principles of tissue heating in thermal ablation, clinical and laboratory parameters for ablation therapy, preprocedural management, imaging assessments of responses, and early adverse events. We also discuss associated challenges and how they may be overcome using optimized imaging techniques.
Selective Activation of M1 Muscarinic Receptors Attenuates Human Colon Cancer Cell Proliferation
Authors: Sundel Margaret H. , Sampaio Moura Natalia , Cheng Kunrong , Chatain Oscar , Hu Shien , Drachenberg Cinthia B. , Xie Guofeng , Raufman Jean-Pierre
Published: September 2023

M3 muscarinic receptor (M3R) activation stimulates colon cancer cell proliferation, migration, and invasion; M3R expression is augmented in colon cancer and ablating M3R expression in mice attenuates colon neoplasia. Several lines of investigation suggest that in contrast to these pro-neoplastic effects of M3R, M1R plays an opposite role, protecting colon epithelial cells against neoplastic transformation. To pursue these intriguing findings, we examined the relative expression of M1R versus M3R in progressive stages of colon neoplasia and the effect of treating colon cancer cells with selective M1R agonists. We detected divergent expression of M1R and M3R in progressive colon neoplasia, from aberrant crypt foci to adenomas, primary colon cancers, and colon cancer metastases. Treating three human colon cancer cell lines with two selective M1R agonists, we found that in contrast to the effects of M3R activation, selective activation of M1R reversibly inhibited cell proliferation. Moreover, these effects were diminished by pre-incubating cells with a selective M1R inhibitor. Mechanistic insights were gained using selective chemical inhibitors of post-muscarinic receptor signaling molecules and immunoblotting to demonstrate M1R-dependent changes in the activation (phosphorylation) of key downstream kinases, EGFR, ERK1/2, and p38 MAPK. We did not detect a role for drug toxicity, cellular senescence, or apoptosis in mediating M1R agonist-induced attenuated cell proliferation. Lastly, adding M1R-selective agonists to colon cancer cells augmented the anti-proliferative effects of conventional chemotherapeutic agents. Collectively, these results suggest that selective M1R agonism for advanced colon cancer, alone or in combination with conventional chemotherapy, is a therapeutic strategy worth exploring.
High-Content and High-Throughput Clonogenic Survival Assay Using Fluorescence Barcoding
Authors: Qian Haibin , Baglamis Selami , Redeker Fumei , Raaijman Julia , Hoebe Ron A. , Sheraton Vivek M. , Vermeulen Louis , Krawczyk Przemek M.
Published: September 2023

The Clonogenic Survival Assay (CSA) is a fundamental tool employed to assess cell survival and proliferative potential in cancer research. Despite its importance, CSA faces limitations, primarily its time- and labor-intensive nature and its binary output. To overcome these limitations and enhance CSA’s utility, several approaches have been developed, focusing on increasing the throughput. However, achieving both high-content and high-throughput analyses simultaneously has remained a challenge. In this paper, we introduce LeGO-CSA, an extension of the classical CSA that employs the imaging of cell nuclei barcoded with fluorescent lentiviral gene ontology markers, enabling both high-content and high-throughput analysis. To validate our approach, we contrasted it with results from a classical assay and conducted a proof-of-concept screen of small-molecule inhibitors targeting various pathways relevant to cancer treatment. Notably, our results indicate that the classical CSA may underestimate clonogenicity and unveil intriguing aspects of clonal cell growth. We demonstrate the potential of LeGO-CSA to offer a robust approach for assessing cell survival and proliferation with enhanced precision and throughput, with promising implications for accelerating drug discovery and contributing to a more comprehensive understanding of cellular behavior in cancer.
COVID-19 Infection despite Previous Vaccination in Cancer Patients and Healthcare Workers: Results from a French Prospective Multicenter Cohort (PAPESCO-19)
Authors: Seegers Valérie , Rousseau Guillaume , Zhou Ke , Blanc-Lapierre Audrey , Bigot Frédéric , Mahammedi Hakim , Lambert Aurélien , Moreau-Bachelard Camille , Campone Mario , Conroy Thierry , Penault-Llorca Frédérique , Bellanger Martine M. , Raoul Jean-Luc
Published: September 2023

In a multicenter prospective cohort of cancer patients (CP; n = 840) and healthcare workers (HCWs; n = 935) vaccinated against COVID-19, we noticed the following: i/after vaccination, 4.4% of HCWs and 5.8% of CP were infected; ii/no characteristic was associated with post-vaccine COVID-19 infections among HCWs; iii/CP who developed infections were younger, more frequently women (NS), more frequently had gastrointestinal, gynecological, or breast cancer and a localized cancer stage; iv/CP vaccinated while receiving chemotherapy or targeted therapy had (NS) more breakthrough infections after vaccination than those vaccinated after these treatments; the opposite was noted with radiotherapy, immunotherapy, or hormonotherapy; v/most COVID-19 infections occurred either during the Alpha wave (11/41 HCW, 20/49 CP), early after the first vaccination campaign started, or during the Omicron wave (21/41 HCW, 20/49 CP), more than 3 months after the second dose; vi/risk of infection was not associated with values of antibody titers; vii/the outcome of these COVID-19 infections after vaccination was not severe in all cases. To conclude, around 5% of our CPs or HCWs developed a COVID-19 infection despite previous vaccination. The outcome of these infections was not severe.
A Survey of the Wild Giant Panda Population and Habitat Reflects an Urgent In Situ Conservation Need: A Case of Meigu Dafengding National Nature Reserve
Authors: Zhang Yiqiong , Wei Wei , Qubi Shibu , Chen Minghua , Gong Yihua , Zhou Hong , Zhang Zejun , Yuan Feiyun , Han Han
Published: September 2023

An important tool for conserving species and their habitats and achieving sustainable development is the establishment of national parks. The population ecology of the species to be conserved, including their population size and suitable habitat, needs to be assessed prior to integrating protected areas into national parks. The Liangshan Mountains are currently the southernmost habitat of giant pandas and lie outside the newly established Giant Panda National Park (GPNP). The population is threatened with extinction due to severe isolation and human disturbance. However, there has been a ten-year gap in the census of giant pandas in this critical area. This means that conservation managers are unable to keep up to date with population and habitat dynamics. The Meigu Dafengding National Nature Reserve is the core area of the giant panda population in Liangshan and the link for gene exchange. The focus of this study is to assess the spatial distribution pattern of suitable habitat, habitat fragmentation and the habitat selection characteristics of giant pandas in the Meigu Dafengding Nature Reserve in Liangshan in order to lay the foundation for the future inclusion of Liangshan into the national park. A total of 151 giant panda fecal samples were collected in 2023. Using the distance–bamboo stem fragments method, this indicated that nearly 28 to 29 giant pandas exist within the reserve. Based on MaxEnt and FRAGSTATS, the giant panda population is concentrated in Wahei–Yiziyakou. They tend to choose gentle slopes of 10–30°, at altitudes between 2500 m and 3500 m, with average annual temperatures between 8.5 °C and 10 °C. They also select the areas close to roads and settlements for their frequent activities. Unsuitable habitat is widespread and surrounds low-, medium- and high-suitability habitats with poor habitat connectivity. In situ conservation measures are urgently needed due to the restricted distribution of populations and poor habitat suitability. This lays the foundation for the future inclusion of Liangshan into the national park, providing greater protection for the giant panda and other species in the region, and for other national parks to integrate all habitats into a single management unit. This will address conservation gaps and overlapping management, and promote the conservation of rare or endangered species.
Integrated Geomodel Accuracy Enhancement Based on Embedded MPS Geological Modeling for Thin Interbedded Reservoirs
Authors: Ke Ling , Ruan Fengming , Duan Taizhong , Li Zhiping , Wang Xiangzeng , Zhao Lei
Published: September 2023

Continental delta deposits are characterized by strong heterogeneity in the lateral direction; meanwhile, reservoir development is challenged by rapid changes in rock properties. Thus, it is critical to use proper methods for fine characterization to confirm the distributions of thin interbedded reservoirs. The aim of this study was to propose a novel workflow for integrated research on the 3D geomodeling of thin interbedded reservoirs, using the Triassic T2a1 formation in the Tahe Oilfield B9 area of the Tarim Basin as a case study. The complicated representation of thin interbeds in a 3D geomodel was simulated using a multiscale joint controlling strategy, based on wells (Points), 2D geological cross-sections (Lines), and horizontal wells (Surfaces). The resistivity inversion results from the horizontal wells validated the proof of the plane distribution of the thin interbeds within the drilled area, and this quantitative statistic provided effective parameters and guidance for 3D interbed geomodeling. In this study, comprehensive 3D facies modeling was divided into 3D interbed geomodeling and 3D sedimentary facies modeling. An optimized interbed geomodel was picked out from multiple stochastic simulation realizations, and the drilled horizontal well data were used to constrain the simulation process, so the simulation results were more consistent with the real distribution of the thin interbed morphology. Classical two-point geostatistical methods, the multipoint simulation (MPS) geostatistical method, and the hierarchical mindset were integrated for the microfacies simulation. This procedure demonstrated a good ability to characterize thin interbed reservoirs in continental delta deposits. An MPS training image obtained from a high-resolution satellite photo was used to fix the issue of the relationships between the distributions and configurations of all microfacies within the spatial distribution. A 3D lithofacies interbed model was embedded into the 3D facies model. This comprehensive facies model served as a constraint condition in the property modeling process. A porosity model was simulated using separate stratigraphy and individual microfacies controls, as facies-controlled property modeling has been used as a prior foundation for field development planning in the Tahe Oilfield B9 case. The porosity model was then used as a basis for permeability modeling, and a water saturation model was created using the J function and all of the constraints from the other two property models. Finally, all the results were validated using dynamic production data from the Tahe Oilfield B9 wells, with good matching observed between the geological models. There was only a 0.92% difference in reservoir volume between the reservoir simulation results and the static geological model results using our solution.
Response of Hydraulic and Photosynthetic Characteristics of Caroxylon passerinum (Bunge) Akhani and Roalson to Prolonged Drought and Short-Term Rehydration
Authors: Wang Yunxia , Wang Hongyong , Xie Tingting , Niu Furong , He Cai , Wang Jianbo , Shan Lishan
Published: September 2023

Hydraulic traits are essential functional characteristics of plants related to water absorption, transport, and loss, serving as indicators of a plant’s adaptability to prevailing environmental water conditions. However, the hydraulic traits of shrub, particularly desert plants in arid and semi-arid regions, have been underexplored. In this study, we conducted a pot experiment using Caroxylon passerinum (Bunge) Akhani and Roalson as the subject. Three treatment groups were established: adequate water supply, mild drought, and severe drought. After subjecting the shrub to drought and subsequent rehydration, we measured hydraulic conductivity, net photosynthetic rate, and stomatal conductance. We found that leaf water potential decreased and stomatal conductance and net photosynthesis decreased with increasing drought intensity. We found that leaf water potential and stomatal conductance decreased with increasing drought intensity. Although there was no significant change in hydraulic conductivity in the two drought groups, the values were greater in the drought group than in the control, and greater in the mild drought group than in the severe drought group. Meanwhile, the embolism resistance decreased with increasing drought intensity. After rehydration, hydraulic conductivity did not return to control levels in the severe drought group, as did embolism resistance in the two drought groups, and leaf water potential did not recover significantly. The results showed that drought stress increased the hydraulic conductivity of C. passerinum, and this effect was more pronounced under mild drought stress. After the stress was lifted, C. passerinum continued to maintain a lower leaf water potential to promote water uptake. This result provides a reference for us to study water use of desert shrubs under different drought stresses.
Prospects for the Preservation of the Main Pinus sylvestris L. Ecotypes in Poland in the Context of the Habitat Conditions of Their Occurrence
Authors: Konatowska Monika , Młynarczyk Adam , Rutkowski Paweł
Published: September 2023

This study investigates the prospects for preserving the main Pinus sylvestris L. (Scots pine) ecotypes in Poland, considering the habitat conditions of their occurrence. Scots pine is known for its wide distribution and natural adaptability to various habitats. However, there is an increasing vulnerability of pine forests to damage from biotic factors and a decrease in natural regeneration, particularly in areas under legal protection. Additionally, projected climate change has raised concerns about the future of Pinus sylvestris, placing it in the “losing” group of tree species. The aim of the study was to analyze the habitat conditions of the seven main selected Pinus sylvestris L. ecotypes to assess the sustainability of pine stands in their natural habitat conditions. Out of the seven populations of studied pine ecotypes, only one grows under conditions representing a typical form of pine forest (Leucobryo–Pinetum plant association). Two populations grow under conditions corresponding to potential deciduous forests (Galio sylvatici–Carpinetum and Calamagrostio arundinaceae–Quercetum petraeae). The remaining populations represent potentially mixed oak–pine forests. Such a distribution of plant communities, except for Leucobryo–Pinetum, does not guarantee the continuity of the studied pine stands as a result of their natural regeneration. Therefore, it is necessary to preserve the offspring of the studied populations outside their occurrence sites, but the studied pine stands should be preserved until their natural death in their natural habitats. In the conducted research, the NDVI turned out to be very useful, showing a high correlation with the trophicity of habitat expressed in the diversity of plant communities, as well as with the height and diameter of the studied stands.
Application of an Effective Hierarchical Deep-Learning-Based Object Detection Model Integrated with Image-Processing Techniques for Detecting Speed Limit Signs, Rockfalls, Potholes, and Car Crashes
Authors: Chung Yao-Liang
Published: September 2023

Against the backdrop of rising road traffic accident rates, measures to prevent road traffic accidents have always been a pressing issue in Taiwan. Road traffic accidents are mostly caused by speeding and roadway obstacles, especially in the form of rockfalls, potholes, and car crashes (involving damaged cars and overturned cars). To address this, it was necessary to design a real-time detection system that could detect speed limit signs, rockfalls, potholes, and car crashes, which would alert drivers to make timely decisions in the event of an emergency, thereby preventing secondary car crashes. This system would also be useful for alerting the relevant authorities, enabling a rapid response to the situation. In this study, a hierarchical deep-learning-based object detection model is proposed based on You Only Look Once v7 (YOLOv7) and mask region-based convolutional neural network (Mask R-CNN) algorithms. In the first level, YOLOv7 identifies speed limit signs and rockfalls, potholes, and car crashes. In the second level, Mask R-CNN subdivides the speed limit signs into nine categories (30, 40, 50, 60, 70, 80, 90, 100, and 110 km/h). The images used in this study consisted of screen captures of dashcam footage as well as images obtained from the Tsinghua-Tencent 100K dataset, Google Street View, and Google Images searches. During model training, we employed Gaussian noise and image rotation to simulate poor weather conditions as well as obscured, slanted, or twisted objects. Canny edge detection was used to enhance the contours of the detected objects and accentuate their features. The combined use of these image-processing techniques effectively increased the quantity and variety of images in the training set. During model testing, we evaluated the model’s performance based on its mean average precision (mAP). The experimental results showed that the mAP of our proposed model was 8.6 percentage points higher than that of the YOLOv7 model—a significant improvement in the overall accuracy of the model. In addition, we tested the model using videos showing different scenarios that had not been used in the training process, finding the model to have a rapid response time and a lower overall mean error rate. To summarize, the proposed model is a good candidate for road safety detection.
Terlipressin for the Prevention and Treatment of Renal Decline in Hepatorenal Syndrome: A Drug Profile
Authors: Ayyad Ahlam , Al-Horani Rami A.
Published: September 2023

Hepatorenal syndrome stands as one of several potential triggers of acute kidney injury in individuals grappling with either acute or persistent liver ailments. The nature of the decline in kidney function has led to the identification of two variants of hepatorenal syndrome. In cases where terlipressin therapy is accessible, the initial approach involves administering terlipressin alongside albumin. Terlipressin, a synthetic analog of vasopressin, boasts double the preference for vasopressin V1 receptors compared to V2 receptors. The FDA granted approval to terlipressin in September 2022, demonstrating its intrinsic activity, although a significant portion of its function arises from its transformation into lysine vasopressin. This article provides a comprehensive examination of terlipressin’s various pharmacodynamic and pharmacokinetic facets, as well as its clinical utility, aiming to keep the scientific community well informed about its safe and efficient utilization.
Transcriptome Analysis of Streptococcus mutans Quorum Sensing-Mediated Persisters Reveals an Enrichment in Genes Related to Stress Defense Mechanisms
Authors: Dufour Delphine , Li Haowen , Gong Siew-Ging , Lévesque Céline M.
Published: September 2023

Persisters are a small fraction of growth-arrested phenotypic variants that can survive lethal concentrations of antibiotics but are able to resume growth once antibiotics are stopped. Their formation can be a stochastic process or one triggered by environmental cues. In the human pathogen Streptococcus mutans, the canonical peptide-based quorum-sensing system is an inducible DNA repair system that is pivotal for bacterial survival. Previous work has shown that the CSP-signaling peptide is a stress-signaling alarmone that promotes the formation of stress-induced persisters. In this study, we exposed S. mutans to the CSP pheromone to mimic DNA damage conditions and isolated the antibiotic persisters by treating the cultures with ofloxacin. A transcriptome analysis was then performed to evaluate the differential gene expression between the normal stationary-phase cells and the persisters. RNA sequencing revealed that triggered persistence was associated with the upregulation of genes related to several stress defense mechanisms, notably, multidrug efflux pumps, the arginine deaminase pathway, and the Opu/Opc system. In addition, we showed that inactivation of the VicK kinase of the YycFG essential two-component regulatory system abolished the formation of triggered persisters via the CSP pheromone. These data contribute to the understanding of the triggered persistence phenotype and may suggest new therapeutic strategies for treating persistent streptococcal infections.
Hourly Associations between Heat Index and Heat-Related Emergency Medical Service (EMS) Calls in Austin-Travis County, Texas
Authors: Seong Kijin , Jiao Junfeng , Mandalapu Akhil
Published: September 2023

This paper aims to investigate the following research questions: (1) what are the hourly patterns of heat index and heat-related emergency medical service (EMS) incidents during summertime?; and (2) how do the lagged effects of heat intensity and hourly excess heat (HEH) vary by heat-related symptoms? Using the hourly weather and heat-related EMS call data in Austin-Travis County, Texas, this paper reveals the relationship between heat index patterns on an hourly basis and heat-related health issues and evaluates the immediate health effects of extreme heat events by utilizing a distributed lag non-linear model (DLNM). Delving into the heat index intensity and HEH, our findings suggest that higher heat intensity has immediate, short-term lagged effects on all causes of heat-related EMS incidents, including in cardiovascular, respiratory, neurological, and non-severe cases, while its relative risk (RR) varies by time. HEH also shows a short-term cumulative lagged effect within 5 h in all-cause, cardiovascular, and non-severe symptoms, while there are no statistically significant RRs found for respiratory and neurological cases in the short term. Our findings could be a reference for policymakers when devoting resources, developing extreme heat warning standards, and optimizing local EMS services, providing data-driven evidence for the effective deployment of ambulances.
Influence of Drying Technique on Physicochemical Properties of Synthetic Hydroxyapatite and Its Potential Use as a Drug Carrier
Authors: Niziołek Karina , Słota Dagmara , Sadlik Julia , Łachut Emilia , Florkiewicz Wioletta , Sobczak-Kupiec Agnieszka
Published: September 2023

Naturally occurring hydroxyapatite (HA) is the mineral phase of bone tissue. It is characterized by its bioactivity toward stimulating bone cells to proliferate and thus form new apatite layers. For this reason, it is a material commonly used in implantology for filling defects or covering implants (such as endoprostheses). There are several methods to obtain synthetic HA, and by controlling parameters such as temperature, pressure or the drying process, physicochemical parameters of the final powder can be affected. In the present study, HA was obtained by wet precipitation technique and subjected to two different drying methods, determining whether this parameter significantly affects the properties of the final material obtained. Analyzed Fourier-transform infrared spectroscopy (FT-IR) confirmed the presence of functional groups typical for HA. X-ray diffraction analysis (XRD) demonstrated that the materials are partially amorphous; however, the only phase was identified in HA. Scanning electron microscopy (SEM) was used to evaluate the surface morphology and the density, and average grain diameter was measured. Furthermore, HA powders were subjected to modification with the antibiotic clindamycin to determine the potential for use as a carrier for the active substance. The release rate of the drug was determined by high-performance liquid chromatography (HPLC). The differences in the characteristics of the powders were relatively small; however, they affected the rate of drug release from the material as well as the shape of the grains. The method of drying the powders was shown to affect the shape of the grains, as well as the porosity of the sinters prepared from it. A higher amount of clindamycin released into PBS was observed in material with more pores. The materials have demonstrated the potential to be used as a carrier for the active substance; however, further biological, as well as physicochemical, analysis is required.
Pioneering Enhanced Corrosion Resistance along the Normal Plane of an Ultra-Light Mg-Li Extruded Sheet
Authors: Liang Jiexi , Deng Binbin , Li Chuanqiang , Dong Yong , Wang Naiguang , Zhang Zhengrong , Wang Shidong
Published: September 2023

The microstructure and corrosion anisotropy of the Mg-5Li extruded sheet were investigated in this work. Three distinct samples cut from the normal plane (A), longitudinal plane (B), and cross-sectional plane (C) of the as-extruded sheet were prepared. The microstructure was analyzed using optical microscopy (OM), scanning electron microscopy (SEM), and X-ray diffraction (XRD). The corrosion resistance and behaviors of the three samples in a 0.1 mol/L NaCl solution were evaluated by employing hydrogen evolution, mass loss testing, electrochemical assessments, and corrosion morphology analyses. The results revealed that sample A displayed a distinctive bimodal (0002) basal texture, along with clearly distinguishably larger grain sizes than the other samples. The effect of grain size and crystallographic orientation on the corrosion resistance was highlighted, indicating the pioneering corrosion resistance of sample A and the lowest corrosion resistance of sample C. Furthermore, all three samples exhibited the characteristic filiform corrosion during the initial stages of corrosion, progressing into the formation of corrosion pits, with sample C displaying pronounced susceptibility.
Translucency of Lithium-Based Silicate Glass–Ceramics Blocks for CAD/CAM Procedures: A Narrative Review
Authors: Vichi Alessandro , Zhao Zejiao , Mutahar Mahdi , Paolone Gaetano , Louca Chris
Published: September 2023

Amid chairside CAD/CAM materials, the use of lithium-based silicate glass–ceramics (LSGC) has been steadily increasing. This review aims to report on the translucency of these materials and the variables used to measure it. An electronic search was performed within the PubMed database within the period between 2 June 2011 and 11 September 2022. English-language papers investigating the translucency of IPS e.max CAD, Celtra Duo, Suprinity PC, Initial LiSi Block, Amber Mill, N!ce, and CEREC Tessera LSGC CAD/blocks were included in the search strategy. After an initial retrieval of 160 papers, the application of exclusion criteria, and the screening of abstracts and then of full texts, 33 papers were included in the study. The retrieved materials, with different degrees of translucency (LT, HT), were IPS e.max CAD (n = 33), Suprinity PC (n = 8), and Celtra Duo (n = 1). Concerning the examined colors, the most used was A2 (n = 20), followed by A1 (n = 8) and A3 (n = 2). The translucency parameter (TP) was the most used method (n = 30) with respect to the contrast ratio (CR) (n = 11) to assess translucency. Five papers measured both. Several specimens’ thicknesses (0.5–4 mm) were investigated, with 1 mm (n = 23) being the most frequently analyzed. While a general tendency could be identified, conflicting results among different papers were reported.
Formation of Oriented Nanowires from Mixed Metal Oxides
Authors: Dikovska Anna , Atanasova Genoveva , Nikov Rumen , Avdeev Georgi , Cherkezova-Zheleva Zara , Paneva Daniela , Nedyalkov Nikolay
Published: September 2023

In this study, we present a physical method for the fabrication of oriented nanowires composed of mixed metal oxides. Pulsed laser deposition carried out in the air under atmospheric pressure was used for the production of samples. Two sets of experiments were performed by applying nanosecond and picosecond laser ablation, respectively. The depositions were performed using the laser ablation of mixed targets from iron oxide and zinc oxide as the initial materials in different ratios. The experiments were carried out in a magnetic field, which allowed us to control the morphology of nanostructures. The structure, microstructure, morphology, and composition of the structures obtained were studied in relation to the sample composition and laser ablation regime applied. The morphological analysis revealed that the structure of the samples consisted mainly of nanowire-like features reaching tens of micrometers in length. These nanowires were composed of nanoparticles and oriented predominantly in parallel to magnetic field lines. Nanoparticles produced using ps ablation were, on average, smaller than those obtained by ns ablation of the same target. Using ablation with ps laser pulses, we were able to produce new composite materials or materials containing unstable phases.
Effect of Composition Adjustment on the Thermoelectric Properties of Mg3Bi2-Based Thermoelectric Materials
Authors: Yang Jianbao , Wang Zhibin , Zhao Hong , Luo Xinyu , Han Wenyuan , Wang Hao , Meng Linghao , She Xinqi , Quan Anlong , Peng Yixin , Cai Guoji , Liu Yi , Tang Yong , Feng Bo
Published: September 2023

Thermoelectric materials are widely used in refrigeration chips, thermal power generation, catalysis and other fields. Mg3Bi2-based thermoelectric material is one of the most promising thermoelectric materials. Herein, the Mg3Bi2-based samples were prepared by high temperature synthesis, and the influence of Mg/Sb content on the electrical transport properties and semi-conductivity/semi-metallicity of the materials has been studied. The results indicate that the efficiency of introducing electrons from excess Mg prepared by high temperature synthesis is lower than that introduced by ball milling, due to the high vapor pressure of Mg. The doping of Sb/Te at the Bi site would make it easier for the material to change from p-type conduction to n-type conduction. With the increase in Mg content, the semi-conductivity of the material becomes weaker, the semi-metallicity becomes stronger, and the corresponding conductivity increases. With the increase in Sb content, the samples exhibit the opposite changes. The highest power factor of ~1.98 mWm−1K−2 is obtained from the Mg3.55Bi1.27Sb0.7Te0.03 sample.
A Swiss-Roll-Type Methanol Mini-Steam Reformer for Hydrogen Generation with High Efficiency and Long-Term Durability
Authors: Tseng Fan-Gang , Chiu Wei-Cheng , Huang Po-Jung
Published: September 2023

This paper proposes a Swiss-roll-type mini-reformer employing a copper–zinc catalyst for high-efficient SRM process. Although the commercially available copper–zinc catalysts commonly used in cylindrical-type reformers provide decent conversion rates in the short term, their long-term durability still requires improvement, mainly due to temperature variations in the reformer, catalyst loading, and thermal sintering issues. This Swiss-roll-shaped mini-reformer is designed to improve thermal energy preservation/temperature uniformity by using dual spiral channels to improve the long-term durability while maintaining methanol-reforming efficiency. It was fabricated on a copper plate that was 80 mm wide, 80 mm long, and 4 mm high with spiral channels that were 2 mm deep, 4 mm wide, and 350 mm long. To optimize the design and reformer operation, the catalyst porosity, gas hourly speed velocity (GHSV), operation temperature, and fuel feeding rate are investigated. Swiss-roll-type reformers may require higher driving pressures but can provide better thermal energy preservation and temperature uniformity, posing a higher conversion rate for the same amount of catalyst when compared with other geometries. By carefully adjusting the catalyst bed porosity, locations, and catalyst loading amount as well as other conditions, an optimized gas hourly space velocity (GHSV) can be obtained (14,580 mL/g·h) and lead to not only a high conversion rate (96%) and low carbon monoxide generation rate (0.98%) but also a better long-term durability (decay from 96% to 88.12% after 60 h operation time) for SRM processes. The decay rate, 0.13%/h, after 60 h of operation, is five-folds lower than that (0.67%/h, 0.134%/h) of a commercial cylindrical-type fixed-bed reactor with a commercial catalyst.
Exploring Copper Oxide and Copper Sulfide for Non-Enzymatic Glucose Sensors: Current Progress and Future Directions
Authors: Miya Nonkululeko , Machogo-Phao Lerato F. Eugeni , Ntsendwana Bulelwa
Published: September 2023

Millions of people worldwide are affected by diabetes, a chronic disease that continuously grows due to abnormal glucose concentration levels present in the blood. Monitoring blood glucose concentrations is therefore an essential diabetes indicator to aid in the management of the disease. Enzymatic electrochemical glucose sensors presently account for the bulk of glucose sensors on the market. However, their disadvantages are that they are expensive and dependent on environmental conditions, hence affecting their performance and sensitivity. To meet the increasing demand, non-enzymatic glucose sensors based on chemically modified electrodes for the direct electrocatalytic oxidation of glucose are a good alternative to the costly enzymatic-based sensors currently on the market, and the research thereof continues to grow. Nanotechnology-based biosensors have been explored for their electronic and mechanical properties, resulting in enhanced biological signaling through the direct oxidation of glucose. Copper oxide and copper sulfide exhibit attractive attributes for sensor applications, due to their non-toxic nature, abundance, and unique properties. Thus, in this review, copper oxide and copper sulfide-based materials are evaluated based on their chemical structure, morphology, and fast electron mobility as suitable electrode materials for non-enzymatic glucose sensors. The review highlights the present challenges of non-enzymatic glucose sensors that have limited their deployment into the market.
An Inspection Technique Using Fit Clearance Based on Microscopic Vision in Precision Assembly
Authors: Li Yawei , Luo Yi , Wang Xiaodong
Published: September 2023

Inspection is a crucial process to ensure product quality. In the precision assembly of an optic-mechanical device, a part with micro multi-section arcs needs to be inspected and assembled into another part. Actually, because of machining errors, including dimensional and geometric shapes, can lead to complex deformation modes for parts with micro multi-section arcs, posing challenges to their inspection. Furthermore, inconsistencies in feature images in microscopic vision may complicate the extraction of the Region of Interest (ROI). To address these issues, this paper proposes an ROI extraction method based on the CAD model for rough positioning of feature points and connected region detection for refinement. Subsequently, based on feature points, the CAD model is used again to obtain the ROI. For inspection purposes, this paper proposes a method suitable for micro multi-section arcs based on assembly fit requirements. Experimental testing was performed on parts with eight-section arcs and mirrors to verify the effectiveness of the proposed method. This method provides a suitable solution for the inspection of micro multi-section arcs in precision assembly with the potential to improve the accuracy of the inspection results.
Effect of Microwave Annealing on the Sensing Characteristics of HfO2 Thin Film for High Sensitive pH-EGFET Sensor
Authors: Cui Siwei , Yang Hui , Zhang Yifei , Su Xing , Wu Dongping
Published: September 2023

Recently, certain challenges have persisted in PH sensor applications, especially when employing hafnium oxide (HfO2) thin films as sensing layers, where issues related to sensitivity, hysteresis, and long-term stability hamper performance. Microwave annealing (MWA) technology, as a promising solution for addressing these challenges, has gained significant attraction due to its unique advantages. In this article, the effects of microwave annealing (MWA) treatment on the sensing behaviors of Extended-Gate Field-Effect Transistors (EGFETs) utilizing HfO2 as a sensing film have been investigated for the first time. Various power levels of MWA treatment (1750 W/2100 W/2450 W) were selected to explore the optimal processing conditions. A thorough physical analysis was conducted to characterize the surface of the MWA-treated HfO2 sensing thin film using techniques such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). Our findings reveal that MWA treatment effectively increased the surface sites (Ns) in the HfO2 sensing thin film, consequently leading to an increase in the pH sensitivity of EGFETs to 59.6 mV/pH, as well as a reduction in hysteresis and an enhancement in long-term stability. These results suggest that MWA offers a straightforward, energy-efficient method to enhance overall HfO2 sensing film performance in EGFETs, offering insights for HfO2 applications and broader microelectronics challenges.
A Novel Mechanomyography (MMG) Sensor Based on Piezo-Resistance Principle and with a Pyramidic Microarray
Authors: Fang Qize , Cao Shuchen , Qin Haotian , Yin Ruixue , Zhang Wenjun , Zhang Hongbo
Published: September 2023

Flexible piezoresistive sensors built by printing nanoparticles onto soft substrates are crucial for continuous health monitoring and wearable devices. In this study, a mechanomyography (MMG) sensor was developed using a flexible piezoresistive MMG signal sensor based on a pyramidal polydimethylsiloxane (PDMS) microarray sprayed with carbon nanotubes (CNTs). The experiment was conducted, and the results show that the sensitivity of the sensor can reach 0.4 kPa−1 in the measurement range of 0~1.5 kPa, and the correlation reached 96%. This has further implications for the possibility that muscle activation can be converted into mechanical movement. The integrity of the sensor in terms of its MMG signal acquisition was tested based on five subjects who were performing arm bending and arm extending movements. The results of this test were promising.
Editorial for the Special Issue on Piezoelectric Transducers: Materials, Devices and Applications, Volume III
Authors: Sanchez-Rojas Jose
Published: September 2023

This is the third volume of a Special Issue focused on piezoelectric transducers, covering a wide range of topics, including the design, fabrication, characterization, packaging and system integration or final applications of mili/micro/nano-electro-mechanical system-based transducers featuring piezoelectric materials and devices [...]
A Rapid and Quantitative Method for Determining Seed Viability Using 2,3,5-Triphenyl Tetrazolium Chloride (TTC): With the Example of Wheat Seed
Authors: Wang Shuonan , Wu Mengmeng , Zhong Sunyaxin , Sun Jing , Mao Xinyue , Qiu Nianwei , Zhou Feng
Published: September 2023

Current colorimetric methods for quantitative determination of seed viability (SV) with 2,3,5-triphenyl tetrazolium chloride (TTC) have been plagued by issues of being cumbersome and time-consuming during the experimental process, slow in extraction and staining, and exhibiting inconsistent results. In this work, we introduced a new approach that combines TTC-staining with high-temperature extraction using dimethyl sulfoxide (DMSO). The optimization of the germination stage, TTC-staining method, and 1,3,5-triphenylformazan (TTF) extraction method were meticulously carried out as follows: When the majority of wheat seeds had grown the radicle, and the length of radicles was approximately equal to the seed length (24 h-germination), 2 g germinating seeds were placed into a beaker (20 mL) containing 5 mL 10 g·L−1 TTC solution. The seeds were stained with TTC in the dark at 25 °C for 1 h. Following the staining, 1 mL 1 mol·L−1 H2SO4 was added to stop the reaction for 5 min. The H2SO4 solution was then removed, and the seeds were gently rinsed with deionized water. Subsequently, the TTF produced in the seeds was extracted directly with 5 mL DMSO solution at 55 °C for 1 h. The absorbance of the extract was measured at 483 nm, and the index of SV was calculated according to a predetermined TTC calibration curve and expressed by mg TTC·g−1 (seed)·h−1. The new method has been demonstrated to be rapid, stable, and highly sensitive, as evidenced by the accurate measurement of seed viability with different aging degrees.
Determination of Phytochemical Contents in Extracts from Different Growth Stages of Oroxylum indicum Fruits Using HPLC-DAD and QAMS Methods
Authors: Rojsanga Piyanuch , Schwaiger Stefan , Stuppner Hermann , Sithisarn Pongtip
Published: September 2023

Flavones are major compounds found in several parts of Oroxylum indicum (O. indicum). The quantification of multiple components by one marker (QAMS) method and the high-performance liquid chromatography (HPLC) method were developed for the quantitative analysis of extracts from the young fruits, green mature fruits, dry pod coats and seeds of O. indicum. Oroxin A, oroxin B and chrysin-7-O-glucuronide were identified in the O. indicum extracts. Oroxylin A and 5-hydroxymethylfurfural were isolated and structurally identified from the pod coat and young fruit extracts, respectively. From the HPLC analysis of the seven major flavones in the extracts, baicalin was the major compound in all extracts investigated (0.4–11% w/w of the extract). All flavone contents were low in the young fruit extract (<1% w/w of the extract). The green mature fruit and dry pod coat extracts showed similar constituent compositions. They contained small amounts of baicalin and oroxylin A, which were found only in these two extracts. Oroxylin A could be used as a marker to indicate the maturity of O. indicum fruits, while 5-hydroxymethylfurfural could be used as a marker for the young fruits. Baicalin was found to be a suitable single marker to calculate the contents of all flavones in the O. indicum extracts.
In Vitro Affinity Maturation of Nanobodies against Mpox Virus A29 Protein Based on Computer-Aided Design
Authors: Yu Haiyang , Mao Guanchao , Pei Zhipeng , Cen Jinfeng , Meng Wenqi , Wang Yunqin , Zhang Shanshan , Li Songling , Xu Qingqiang , Sun Mingxue , Xiao Kai
Published: September 2023

Mpox virus (MPXV), the most pathogenic zoonotic orthopoxvirus, caused worldwide concern during the SARS-CoV-2 epidemic. Growing evidence suggests that the MPXV surface protein A29 could be a specific diagnostic marker for immunological detection. In this study, a fully synthetic phage display library was screened, revealing two nanobodies (A1 and H8) that specifically recognize A29. Subsequently, an in vitro affinity maturation strategy based on computer-aided design was proposed by building and docking the A29 and A1 three-dimensional structures. Ligand-receptor binding and molecular dynamics simulations were performed to predict binding modes and key residues. Three mutant antibodies were predicted using the platform, increasing the affinity by approximately 10-fold compared with the parental form. These results will facilitate the application of computers in antibody optimization and reduce the cost of antibody development; moreover, the predicted antibodies provide a reference for establishing an immunological response against MPXV.
NiMoO4 Nanosheets Embedded in Microflake-Assembled CuCo2O4 Island-like Structure on Ni Foam for High-Performance Asymmetrical Solid-State Supercapacitors
Authors: Li Gaofeng , Chen Lingling , Li Longfei
Published: September 2023

Micro/nano-heterostructure with subtle structural design is an effective strategy to reduce the self-aggregation of 2D structure and maintain a large specific surface area to achieve high-performance supercapacitors. Herein, we report a rationally designed micro/nano-heterostructure of complex ternary transition metal oxides (TMOs) by a two-step hydrothermal method. Microflake-assembled island-like CuCo2O4 frameworks and secondary inserted units of NiMoO4 nanosheets endow CuCo2O4/NiMoO4 composites with desired micro/nanostructure features. Three-dimensional architectures constructed from CuCo2O4 microflakes offer a robust skeleton to endure structural change during cycling and provide efficient and rapid pathways for ion and electron transport. Two-dimensional NiMoO4 nanosheets possess numerous active sites and multi-access ion paths. Benefiting from above-mentioned advantages, the CuCo2O4/NiMoO4 heterostructures exhibit superior pseudocapacitive performance with a high specific capacitance of 2350 F/g at 1 A/g as well as an excellent cycling stability of 91.5% over 5000 cycles. A solid-state asymmetric supercapacitor based on the CuCo2O4/NiMoO4 electrode as a positive electrode and activated carbon as a negative electrode achieves a high energy density of 51.7 Wh/kg at a power density of 853.7 W/kg. These results indicate that the hybrid micro/nanostructured TMOs will be promising for high-performance supercapacitors.