bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of precursor-derived peracetic acid on post-weaning diarrhea, intestinal microbiota, and predicted microbial functional genes in weaned pigs
Authors: Galgano Salvatore , Conway Leah , Fellows Adrian , Houdijk Jos
Published: January 2024

Post-weaning diarrhea affects piglets in the nursery phase of production, leading to a substantial impact both at the farm and financial levels. The multifactorial etiology of this disease includes housing conditions, pig genetics, microbial composition, and metagenomic assets. Among the common therapeutic approaches, the widely used zinc oxide underwent a European Union ban in 2022 due to its negative environmental impact and correlation to increased antimicrobial resistance. During this study, we have tested two levels of inclusion of the potential antimicrobial alternative peracetic acid, delivered in water via the hydrolysis of the precursors sodium percarbonate and tetraacetylethylenediamine, in comparison to zinc oxide and an untreated control during a 2-week animal study. We assessed the microbial composition and predicted the metagenome, together with performance and physiological parameters, in order to describe the microbial functional role in etiopathology. Both zinc oxide and peracetic acid resulted in amelioration of the diarrheal status by the end of the trial period, with noticeable zinc oxide effects visible from the first week. This was accompanied by improved performance when compared to the first-week figures and a decreased stomach pH in both peracetic acid levels. A significant reduction in both stomach and caecal Proteobacteria was recorded in the zinc oxide group, and a significant reduction of Campylobacter in the stomach was reported for both zinc oxide and one of the peracetic acid concentrations. Among other functional differences, we found that the predicted ortholog for the zonula occludens toxin, a virulence factor present in pathogens like Escherichia coli and Campylobacter jejuni, was less abundant in the stomach of treated pigs compared to the control group. In water, peracetic acid delivered via precursor hydrolysis has the potential to be a valid intervention, an alternative to antimicrobial, to assist the weaning of piglets. Our findings support the view that post-weaning diarrhea is a complex multifactorial disease with an important metagenomic component characterized by the differential abundance of specific predicted orthologs and microbial genera in the stomach and caecum of pigs.
Positional multi-length and mutual-attention network for epileptic seizure classification
Authors: Zhang Guokai , Zhang Aiming , Liu Huan , Luo Jihao , Chen Jianqing
Published: January 2024

The automatic classification of epilepsy electroencephalogram (EEG) signals plays a crucial role in diagnosing neurological diseases. Although promising results have been achieved by deep learning methods in this task, capturing the minute abnormal characteristics, contextual information, and long dependencies of EEG signals remains a challenge. To address this challenge, a positional multi-length and mutual-attention (PMM) network is proposed for the automatic classification of epilepsy EEG signals. The PMM network incorporates a positional feature encoding process that extracts minute abnormal characteristics from the EEG signal and utilizes a multi-length feature learning process with a hierarchy residual dilated LSTM (RDLSTM) to capture long contextual dependencies. Furthermore, a mutual-attention feature reinforcement process is employed to learn the global and relative feature dependencies and enhance the discriminative abilities of the network. To validate the effectiveness PMM network, we conduct extensive experiments on the public dataset and the experimental results demonstrate the superior performance of the PMM network compared to state-of-the-art methods.
Translational medical bioengineering research of traumatic brain injury among Chinese and American pedestrians caused by vehicle collision based on human body finite element modeling
Authors: Yan Lingbo , Liu Chenyu , Zhu Xiaoming , Zhou Dayong , Lv Xiaojiang , Kuang Xuyuan
Published: January 2024

Based on the average human body size in China and the THUMS AM50 finite element model of the human body, the Kriging interpolation algorithm was used to model the Chinese 50th percentile human body, and the biological fidelity of the model was verified. We built three different types of passenger vehicle models, namely, sedan, sports utility vehicle (SUV), and multi-purpose vehicle (MPV), and used mechanical response analysis and finite element simulation to compare and analyze the dynamic differences and head injury differences between the Chinese 50th percentile human body and the THUMS AM50 model during passenger vehicle collisions. The results showed that there are obvious differences between the Chinese mannequin and THUMS in terms of collision time, collision position, invasion speed, and angle. When a sedan collided with the mannequins, the skull damage to the Chinese human body model was more severe, and when a sedan or SUV collided, the brain damage to the Chinese human body was more severe. The abovementioned results suggest that the existing C-NCAP pedestrian protection testing regulations may not provide the best protection for Chinese human bodies, and that the regulations need to be improved by combining collision damage mechanisms and the physical characteristics of Chinese pedestrians. This thorough investigation is positioned to shed light on the fundamental biomechanics and injury mechanisms at play. Furthermore, the amalgamation of clinically rooted translational and engineering research in the realm of traumatic brain injury has the potential to establish a solid foundation for discerning preventive methodologies. Ultimately, this endeavor holds the potential to introduce effective strategies aimed at preventing and safeguarding against traumatic brain injuries.
Case report: Fatal Borna virus encephalitis manifesting with basal brain and brainstem symptoms
Authors: Lourbopoulos Athanasios , Schnurbus Lea , Guenther Ricarda , Steinlein Susanne , Ruf Viktoria , Herms Jochen , Jahn Klaus , Huge Volker
Published: January 2024

Background

Since the first report of fatal Borna virus-1 (BoDV-1) encephalitis in 2018, cases gradually increased. There is a lack of diagnostic algorithm, and there is no effective treatment so far.

Case presentation

We report an acute BoDV-1 encephalitis in a 77-year-old female with flu-like onset, rapid progression to word-finding difficulties, personality changes, global disorientation, diffuse cognitive slowness, and gait ataxia and further deterioration with fever, meningism, severe hyponatremia, epileptic seizures, cognitive decline, and focal cortical and cerebellar symptoms/signs. The extensive diagnostic workup (cerebrovascular fluid, serum, and MRI) for (meningo-)encephalitis was negative for known causes. Our empirical common antiviral, antimicrobial, and immunosuppressive treatment efforts failed. The patient fell into coma 5 days after admission, lost all brainstem reflexes on day 18, remained fully dependent on invasive mechanical ventilation thereafter and died on day 42. Brain and spinal cord autopsy confirmed an extensive, diffuse, and severe non-purulent, lymphocytic sclerosing panencephalomyelitis due to BoDV-1, affecting neocortical, subcortical, cerebellar, neurohypophysis, and spinal cord areas. Along with our case, we critically reviewed all reported BoDV-1 encephalitis cases.

Conclusion

The diagnosis of acute BoDV-1 encephalitis is challenging and delayed, while it progresses to fatal. In this study, we list all tried and failed treatments so far for future reference and propose a diagnostic algorithm for prompt suspicion and diagnosis.
Causal relationship between gut microbiota and myasthenia gravis: a two-sample Mendelian randomization study
Authors: Mi Chuanhao , Hou Ajiao , Wang Ziyue , Qi Xianghua , Teng Jing
Published: January 2024

Background

Previous observational studies have provided cumulative data linking gut microbiota to myasthenia gravis (MG). However, the causal link between the two remains unexplored. Hence, the current study was performed to explore the causal link between them.

Methods

Mendelian randomization (MR) analysis was conducted using the summary statistics of 211 gut microbiota taxa and the largest genome-wide association studies (GWAS) for MG currently available. The inverse variance-weighted (IVW), MR-Egger, weighted median, and weighted mode methods were employed to ascertain the causal influence. Sensitivity studies utilizing several methodologies were then used to assess the robustness of the findings. Lastly, to evaluate reverse causality, a reverse MR analysis was performed.

Results

Seven suggestive causal associations between the gastrointestinal microbiota and MG were identified based on the outcomes of the MR analysis. Specifically, phylum Actinobacteria (OR: 0.602, 95% CI: 0.405–0.896, p = 0.012), class Gammaproteobacteria (OR: 0.587, 95% CI: 0.357–0.968, p = 0.037), and families Defluviitaleaceae (OR: 0.695, 95% CI: 0.485–0.996, p = 0.047), Family XIII (OR: 0.614, 95% CI: 0.412–0.916, p = 0.017), and Peptococcaceae (OR: 0.698, 95% CI: 0.505–0.964, p = 0.029) had suggestive protective effects on MG, while order Mollicutes RF9 (OR: 1.424, 95% CI: 1.015–1.998, p = 0.041) and genus Faecalibacterium (OR: 1.763, 95% CI: 1.220–2.547, p = 0.003) were suggestive risk factors for MG. The outcomes indicate that neither heterogeneity nor horizontal pleiotropy had any discernible impact. Nevertheless, this reverse analysis did not reveal any apparent effect of MG on the gut microbiota composition.

Conclusion

The MR investigation has substantiated the suggestive causal connection between gut microbiota and MG, which may provide helpful insights for innovative therapeutic and preventative approaches for MG. Further randomized controlled trials are needed to elucidate the gut microbiota’s precise role and therapeutic potential in the pathogenesis of MG.
Validation of general pain scores from multidomain assessment tools in stroke
Authors: Ali Myzoon , Tibble Holly , Brady Marian C. , Quinn Terence J. , Sunnerhagen Katharina S. , Venketasubramanian Narayanaswamy , Shuaib Ashfaq , Pandyan Anand , Mead Gillian
Published: January 2024

Purpose

We describe how well general pain reported in multidomain assessment tools correlated with pain-specific assessment tools; associations between general pain, activities of daily living and independence after stroke.

Materials and methods

Analyses of individual participant data (IPD) from the Virtual International Stroke Trials Archive (VISTA) described correlation coefficients examining (i) direct comparisons of assessments from pain-specific and multidomain assessment tools that included pain, (ii) indirect comparisons of pain assessments with the Barthel Index (BI) and modified Rankin Scale (mRS), and (iii) whether pain identification could be enhanced by accounting for reported usual activities, self-care, mobility and anxiety/depression; factors associated with pain.

Results

European Quality of Life 3- and 5-Level (EQ-5D-3L and EQ-5D-5L), RAND 36 Item Health Survey 1.0 (SF-36) or the 0–10 Numeric Pain Rating Scale (NPRS) were available from 10/94 studies (IPD = 10,002). The 0–10 NPRS was the only available pain-specific assessment tool and was a reference for comparison with other tools. Pearson correlation coefficients between the 0–10 NPRS and (A) the EQ-5D-3L and (B) EQ5D-5 L were r = 0.572 (n = 436) and r = 0.305 (n = 1,134), respectively. mRS was better aligned with pain by EQ-5D-3L (n = 8,966; r = 0.340) than by SF-36 (n = 623; r = 0.318). BI aligned better with pain by SF-36 (n = 623; r = −0.320). Creating a composite score using the EQ-5D 3 L and 5 L comprising pain, mobility, usual-activities, self-care and anxiety/depression did not improve correlation with the 0–10 NPRS.

Discussion

The EQ-5D-3L pain domain aligned better than the EQ-5D-5L with the 0–10 NPRS and may inform general pain description where resources and assessment burden hinder use of additional, pain-specific assessments.
Mononeuritis multiplex following immune checkpoint inhibitors in malignant pleural mesothelioma
Authors: Farina Antonio , Escalere Manon , Dion Matthias , Moussy Martin , Pegat Antoine , Villagrán-García Macarena , Devic Perrine , Lamiral Anaïde , Seyve Antoine , Aure Karine , Wang Adrien , Gorza Lucas , Streichenberger Nathalie , Maisonobe Thierry , Honnorat Jerome , Birzu Cristina , Psimaras Dimitri , Weisenburger-Lile David , Joubert Bastien
Published: January 2024

Introduction

Mononeuritis multiplex is frequently related to vasculitic neuropathy and has been reported only sporadically as an adverse event of immune checkpoint inhibitors.

Methods

Case series of three patients with mononeuritis multiplex—all with mesothelioma—identified in the databases of two French clinical networks (French Reference Center for Paraneoplastic Neurological Syndromes, Lyon; OncoNeuroTox, Paris; January 2015–October 2022) set up to collect and investigate n-irAEs on a nationwide level.

Results

Three patients (male; median age 86 years; range 72–88 years) had pleural mesothelioma and received 10, 4, and 6 cycles, respectively, of first-line nivolumab plus ipilimumab combined therapy. In patient 1, the neurological symptoms involved the median nerves, and in the other two patients, there was a more diffuse distribution; the symptoms were severe (common terminology criteria for adverse events, CTCAE grade 3) in all patients. Nerve conduction studies indicated mononeuritis multiplex in all patients. Peripheral nerve biopsy demonstrated necrotizing vasculitis in patients 1 and 3 and marked IgA deposition without inflammatory lesions in patient 2. Immune checkpoint inhibitors were permanently withdrawn, and corticosteroids were administered to all patients, leading to complete symptom regression (CTCAE grade 0, patient 2) or partial improvement (CTCAE grade 2, patients 1 and 3). During steroid tapering, patient 1 experienced symptom recurrence and spreading to other nerve territories (CTCAE grade 3); he improved 3 months after rituximab and cyclophosphamide administration.

Discussion

We report the occurrence of mononeuritis multiplex, a very rare adverse event of immune checkpoint inhibitors, in the three patients with mesothelioma. Clinicians must be aware of this severe, yet treatable adverse event.
The effects of different intermittent fasting regimens in people with type 2 diabetes: a network meta-analysis
Authors: Xiaoyu Wen , Yuxin Xiao , Li Lai
Published: January 2024

Objective

To compare the effects of four intermittent fasting regimens on blood glucose and insulin sensitivity in people with type 2 diabetes.

Methods

Randomized controlled trials of intermittent fasting in the treatment of patients with type 2 diabetes mellitus in PubMed, the Cochrane Library, Embase, ScienceDirect, Web of Science, CNKI, VIP Database, and WANFANG Database were searched for from the library to September 2023. 2 review authors independently screened studies and extracted data. RevMan 5.4 was used for direct comparison of meta-results. Network meta-analysis was performed using Stata16 software.

Results

13 studies with a total of 867 patients were included. The intervention effects of twice-per-week fasting, fasting-mimicking diet, time-restricted eating, and peridic fasting were better than that of conventional diet. The results of the network comparison showed that there was no significant difference in the intervention effect of the intermittent fasting regimens. SUCRA ranking results showed that the twice-per-week fasting was best for comprehensive interventions for improvement.

Conclusion

From the perspective of fasting blood glucose, glycated hemoglobin and insulin resistance, the twice-per-week fasting intervention has a good effect, which can be used as a reference for patients with inter-type 2 diabetes to choose intermittent fasting regimen. However, more clinical trials are needed to verify this at a later stage.
Daratumumab-based immunotherapy vs. lenalidomide, bortezomib and dexamethasone in transplant-ineligible newly diagnosed multiple myeloma: a systemic review
Authors: Tang Wenjiao , Zhang Li , Zheng Yuhuan , Pan Ling , Niu Ting
Published: January 2024

Background

Since no randomized controlled trials have directly compared the efficacy and safety of immunotherapy with daratumumab versus lenalidomide/bortezomib/dexamethasone (RVD) in the frontline treatment of transplant-ineligible newly diagnosed multiple myeloma (TIE-NDMM), this study systematically reviewed the clinical studies regarding immunotherapy with daratumumab and RVD regimen in the treatment of TIE-NDMM to explore the optimization direction of the best first-line therapy.

Methods

The Cochrane Library, PubMed, Embase, and Web of Science databases were searched to collect studies on regimens containing daratumumab or RVD/RVD-lite for TIE-NDMM. Pooled and meta-analysis was then performed to compare the overall response rate (ORR), stringent complete remission (sCR) and CR rate, progression-free survival (PFS), overall survival (OS) and treatment-related discontinuation rate between daratumumab-containing immunotherapy regimen and RVD/RVD-lite regimen by using R 4.3.1 software.

Results

Nine prospective clinical trials were included, including 1795 TIE-NDMM or NDMM without intent for immediate ASCT. Among them, 938 patients were treated with daratumumab-based immunotherapy and 857 with RVD/RVD-lite regimens. Meta-analysis results showed that The daratumumab-based regimen showed a significantly higher CR/sCR rate than RVD/RVD-lite for TIE-NDMM (47% vs. 24%, P<0.01). The median PFS of the daratumumab-based and RVD/RVD-lite groups were 52.6 months and 35.1 months respectively (HR 0.77, 95%CI, 0.66-0.90). The median OS of both groups was not reached, and there were no significant differences in OS between the two groups (HR 1.03, 95%CI, 0.86-1.23). The therapy discontinuation rate led by adverse events was significantly higher in the RVD/RVD-lite group than in the daratumumab-based regimen group for the TIE-NDMM (16% vs. 7%, P=0.03).

Conclusion

This meta-analysis suggests that daratumumab-containing immunotherapy is superior to RVD in the depth of treatment efficacy, progression-free survival, and lower treatment-related discontinuation rates. Limited by the lack of head-to-head clinical trials, this conclusion needs to be verified by concurrent cohort studies.
mTOR inhibitor introduce disitamab vedotin (RC48-ADC) rechallenge microtubule-chemotherapy resistance in HER2-low MBC patients with PI3K mutation
Authors: Hu Ye , Chen Fengxi , Sun Siwen , Xv Lingzhi , Wang Xueqing , Wang Meiling , Zhao Shanshan , Zhao Zuowei , Li Man
Published: January 2024

This study aimed to explore the efficacy and potential mechanisms of rechallenge therapy with microtubule-targeting agents (MTAs) in patients with HER2-low metastatic breast cancer (MBC). We performed a systematic review to investigate the rechallenge treatment concept in the field of HER2-low MBC treatment and utilized a series of cases identified in the literature to illustrate the concept. Here we reported two clinical cases of HER2-low MBC patients whose disease progressed after prior treatment with MTAs such as docetaxel and vincristine. When rechallenged with disitamab vedotin ((RC48-antibody-drug conjugate (ADC), a monomethyl auristatin (MMAE) MTA)), both patients achieved a partial response and the final progression-free survival (PFS) was 13.5 and 9 months, respectively. Genomic profiling detected a PIK3CA H1047R mutation in the patients. The patients were treated with everolimus before being rechallenged with RC48, which may lead to a better response. This study further summarizes and analyzes the potential mechanism of the PI3K-AKT signaling pathway in MTA resistance and reveals that the PIK3CA H1047R mutation may be a potential molecular marker for the efficacy prediction of mTOR inhibitors, providing new insights and potential therapeutic strategies for the application of MTAs to MBC patients.
Accuracy of radiomics in the diagnosis and preoperative high-risk assessment of endometrial cancer: a systematic review and meta-analysis
Authors: He Junmei , Liu Yurong , Li Jinzhu , Liu Shuang
Published: January 2024

Background

With the increasing use of radiomics in cancer diagnosis and treatment, it has been applied by some researchers to the preoperative risk assessment of endometrial cancer (EC) patients. However, comprehensive and systematic evidence is needed to assess its clinical value. Therefore, this study aims to investigate the application value of radiomics in the diagnosis and treatment of EC.

Methods

Pubmed, Cochrane, Embase, and Web of Science databases were retrieved up to March 2023. Preoperative risk assessment of EC included high-grade EC, lymph node metastasis, deep myometrial invasion status, and lymphovascular space invasion status. The quality of the included studies was appraised utilizing the RQS scale.

Results

A total of 33 primary studies were included in our systematic review, with an average RQS score of 7 (range: 5–12). ML models based on radiomics for the diagnosis of malignant lesions predominantly employed logistic regression. In the validation set, the pooled c-index of the ML models based on radiomics and clinical features for the preoperative diagnosis of endometrial malignancy, high-grade tumors, lymph node metastasis, lymphovascular space invasion, and deep myometrial invasion was 0.900 (95%CI: 0.871–0.929), 0.901 (95%CI: 0.877–0.926), 0.906 (95%CI: 0.882–0.929), 0.795 (95%CI: 0.693–0.897), and 0.819 (95%CI: 0.705–0.933), respectively.

Conclusions

Radiomics shows excellent accuracy in detecting endometrial malignancies and in identifying preoperative risk. However, the methodological diversity of radiomics results in significant heterogeneity among studies. Therefore, future research should establish guidelines for radiomics studies based on different imaging sources.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=364320 identifier CRD42022364320.
Time to recovery from neonatal sepsis and its determinants among neonates admitted in Woldia comprehensive specialized hospital, Northeast Ethiopia: a retrospective cohort study
Authors: Ambaye Kassawmar , Yimer Ali , Mislu Esuyawkal , Wendimagegn Zeru , Kumsa Henok
Published: January 2024

Background

Neonatal sepsis is the most serious problem in neonates. It is the leading cause of neonatal death in developing countries, particularly in sub-Saharan Africa. The Ethiopian 2016 Demographic Health Survey report revealed that a high number of neonatal deaths are associated with neonatal sepsis. However, limited studies are available on exposure and time to recovery inferences in Ethiopia. Therefore, this study aimed to assess the time to recovery from neonatal sepsis and its determinants among neonates admitted to Woldia Comprehensive Specialized Hospital (WCSH), Northeast Ethiopia.

Methods

A retrospective cohort study was conducted, including 351 neonates, using systematic random sampling at WCSH from 7 to 30 March 2023. The data were entered into Epi data version 4.6 and exported to STATA 14 for analysis. Cox regression was used to identify the determinants of time to recovery from neonatal sepsis, and a variable with a p-value of less than 0.05, was used to declare significant association at a 95% confidence interval.

Result

Among 351 neonates with sepsis, 276 (78.63%) recovered, and the median time to recovery was 6 days. Induced labor (AHR = 0.54, 95% CI: 0.369, 0.78) and resuscitation at birth (AHR = 0.7, 95% CI: 0.51, 0.974) were significantly associated with the recovery time of neonatal sepsis.

Conclusions and recommendation

The time to recovery from neonatal sepsis is comparable to previous studies' results. The 25th and 75th percentiles were 4 and 8 days, respectively. Health professionals working in the NICU need to pay special attention to neonates born from mothers who had induced labor and those who were resuscitated at birth.
Strengths and opportunities to clinical trial enrollment among BIPOC, rural dwelling patients in the northwest United States: a retrospective study
Authors: Nelson Jamie M. , Johnson Elizabeth , Kiesow Becky , McCrory Bernadette , Ma Jiahui
Published: January 2024

Introduction: Clinical trials investigating the safety and efficacy of experimental drugs and devices are the cornerstone of medicinal advancement. Enrolling sufficient participants in these trials is vital to ensure adequate statistical power and generalizability. Clinical trial participation is particularly low among certain populations, including medically underserved communities (i.e., rural areas) and Black, Indigenous, and People of Color (BIPOC).

Methods: A retrospective study design was used to understand patient outcomes and access/barriers to clinical trial participation in the rural northwest United States. A quantitatively focused retrospective chart review was conducted for adult participants enrolled in at least one clinical trial in a single northwest health system between 1999 and 2022. Descriptive and inferential statistical analyses were performed to assess trial outcomes at a significance level 0.05.

Results: The retrospective chart review yielded 833 clinical trial records with 753 individual enrolled participants. The all-cause relative frequency of death at last known follow-up amongst clinical trial participants was 8.90% (n = 67). Based on logistic regression, the death was significantly associated with the participants’ age at initial trial screening (β = 0.09, p-value <0.001), those that resided in non-metro areas (β = −0.86, p-value = 0.045), and those that lived in Northeastern Montana (β = 1.27, p-value = 0.025). Additionally, death at last known follow-up was significantly associated with enrollment in 2021–2022 (β = −1.52, p-value <0.001), enrolled in more than one study (β = 0.84, p-value = 0.023), in internationally sponsored trials (β = −2.08, p-value <0.001), in Phase I (β = 5.34, p-value <0.001), in Phase II trials (β = 1.37, p-value = 0.013), diabetes as a primary trial target (β = −2.04, p-value = 0.003).

Conclusion: As decentralized trial design and remote or virtual elements of traditional trials become normative, representation of rural and frontier populations is imperative to support the generalizability of trial data encouraged by the FDA.
A novel dilated contextual attention module for breast cancer mitosis cell detection
Authors: Li Zhiqiang , Li Xiangkui , Wu Weixuan , Lyu He , Tang Xuezhi , Zhou Chenchen , Xu Fanxin , Luo Bin , Jiang Yulian , Liu Xingwen , Xiang Wei
Published: January 2024

Background and object: Mitotic count (MC) is a critical histological parameter for accurately assessing the degree of invasiveness in breast cancer, holding significant clinical value for cancer treatment and prognosis. However, accurately identifying mitotic cells poses a challenge due to their morphological and size diversity.

Objective: We propose a novel end-to-end deep-learning method for identifying mitotic cells in breast cancer pathological images, with the aim of enhancing the performance of recognizing mitotic cells.

Methods: We introduced the Dilated Cascading Network (DilCasNet) composed of detection and classification stages. To enhance the model’s ability to capture distant feature dependencies in mitotic cells, we devised a novel Dilated Contextual Attention Module (DiCoA) that utilizes sparse global attention during the detection. For reclassifying mitotic cell areas localized in the detection stage, we integrate the EfficientNet-B7 and VGG16 pre-trained models (InPreMo) in the classification step.

Results: Based on the canine mammary carcinoma (CMC) mitosis dataset, DilCasNet demonstrates superior overall performance compared to the benchmark model. The specific metrics of the model’s performance are as follows: F1 score of 82.9%, Precision of 82.6%, and Recall of 83.2%. With the incorporation of the DiCoA attention module, the model exhibited an improvement of over 3.5% in the F1 during the detection stage.

Conclusion: The DilCasNet achieved a favorable detection performance of mitotic cells in breast cancer and provides a solution for detecting mitotic cells in pathological images of other cancers.
Translating purpose and mindset into positive impact through shared vision, compassion, and energy—a comparative study of seven organizations
Authors: Leah Joseph S.
Published: January 2024

How do organizations that explicitly state the intention to be “positive impact companies” differ from traditional entrepreneurial companies? How does the quality of relationships in those companies affect the ability to deliver positive impact? This study explores the conditions under which business leaders turn their organizations toward generating prosperity for all stakeholders, achieving positive societal outcomes, improving human wellbeing, and delivering great business results. Seven case study companies are examined based on interviews with three executives from each firm, including the CEO. All seven companies are privately owned small/medium sized businesses from a cross section of industries and diverse geographic bases, ranging from Michigan to the Pacific Northwest, to Singapore, Egypt, and Florida. Four of the companies are considered “positive impact companies” (PICs) based on their organizational affiliations, while the other three are considered traditional entrepreneurial companies (TECs). The overall findings suggest that the core of positive impact leadership resides in a shift in the mindset of leaders toward one of connectedness and purpose, and that these factors influence the quality of relationships in organizations in a positive way whether the company is considered a PIC or a TEC. The results also suggest a close relationship between several core indicators of a fundamental shift in understanding about the role of business in society: shared values of human wellbeing, a common shared vision, an emphasis on collaboration and caring in organizations, and a long-term perspective toward the creation of shared economic prosperity.
Prototyping the implementation of a suicide prevention protocol in primary care settings using PDSA cycles: a mixed method study
Authors: Minian Nadia , Gayapersad Allison , Coroiu Adina , Dragonetti Rosa , Zawertailo Laurie , Zaheer Juveria , O’Neill Braden , Lange Shannon , Thomson Nicole , Crawford Allison , Kennedy Sidney H. , Selby Peter
Published: January 2024

Introduction

In Canada, approximately 4,500 individuals die by suicide annually. Approximately 45% of suicide decedents had contact with their primary care provider within the month prior to their death. Current versus never smokers have an 81% increased risk of death by suicide. Those who smoke have additional risks for suicide such as depression, chronic pain, alcohol, and other substance use. They are more likely to experience adverse social determinants of health. Taken together, this suggests that smoking cessation programs in primary care could be facilitators of suicide prevention, but this has not been studied.

Study objectives

The objectives of the study are to understand barriers/facilitators to implementing a suicide prevention protocol within a smoking cessation program (STOP program), which is deployed by an academic mental health and addiction treatment hospital in primary care clinics and to develop and test implementation strategies to facilitate the uptake of suicide screening and assessment in primary care clinics across Ontario.

Methods

The study employed a three-phase sequential mixed-method design. Phase 1: Conducted interviews guided by the Consolidated Framework for Implementation Research exploring barriers to implementing a suicide prevention protocol. Phase 2: Performed consensus discussions to map barriers to implementation strategies using the Expert Recommendations for Implementing Change tool and rank barriers by relevance. Phase 3: Evaluated the feasibility and acceptability of implementation strategies using Plan Do Study Act cycles.

Results

Eleven healthcare providers and four research assistants identified lack of training and the need of better educational materials as implementation barriers. Participants endorsed and tested the top three ranked implementation strategies, namely, a webinar, adding a preamble before depression survey questions, and an infographic. After participating in the webinar and reviewing the educational materials, all participants endorsed the three strategies as acceptable/very acceptable and feasible/very feasible.

Conclusion

Although there are barriers to implementing a suicide prevention protocol within primary care, it is possible to overcome them with strategies deemed both acceptable and feasible. These results offer promising practice solutions to implement a suicide prevention protocol in smoking cessation programs delivered in primary care settings. Future efforts should track implementation of these strategies and measure outcomes, including provider confidence, self-efficacy, and knowledge, and patient outcomes.
Can adverse childhood experiences predict chronic health conditions? Development of trauma-informed, explainable machine learning models
Authors: Afzal Hanin B. , Jahangir Tasfia , Mei Yiyang , Madden Annabelle , Sarker Abeed , Kim Sangmi
Published: January 2024

Introduction

Decades of research have established the association between adverse childhood experiences (ACEs) and adult onset of chronic diseases, influenced by health behaviors and social determinants of health (SDoH). Machine Learning (ML) is a powerful tool for computing these complex associations and accurately predicting chronic health conditions.

Methods

Using the 2021 Behavioral Risk Factor Surveillance Survey, we developed several ML models—random forest, logistic regression, support vector machine, Naïve Bayes, and K-Nearest Neighbor—over data from a sample of 52,268 respondents. We predicted 13 chronic health conditions based on ACE history, health behaviors, SDoH, and demographics. We further assessed each variable’s importance in outcome prediction for model interpretability. We evaluated model performance via the Area Under the Curve (AUC) score.

Results

With the inclusion of data on ACEs, our models outperformed or demonstrated similar accuracies to existing models in the literature that used SDoH to predict health outcomes. The most accurate models predicted diabetes, pulmonary diseases, and heart attacks. The random forest model was the most effective for diabetes (AUC = 0.784) and heart attacks (AUC = 0.732), and the logistic regression model most accurately predicted pulmonary diseases (AUC = 0.753). The strongest predictors across models were age, ever monitored blood sugar or blood pressure, count of the monitoring behaviors for blood sugar or blood pressure, BMI, time of last cholesterol check, employment status, income, count of vaccines received, health insurance status, and total ACEs. A cumulative measure of ACEs was a stronger predictor than individual ACEs.

Discussion

Our models can provide an interpretable, trauma-informed framework to identify and intervene with at-risk individuals early to prevent chronic health conditions and address their inequalities in the U.S.
The Silicon Cycle in the Ocean
Authors: Cassarino Lucie , Pickering Rebecca A. , Zhang Zhouling , Liguori Bianca
Published: January 2024

The element silicon is everywhere! In fact, silicon is the second most abundant element in Earth’s crust. Silicon in rocks and minerals breaks down and is transported from rivers and streams into the world’s oceans. Many marine organisms need silicon as it is a crucial nutrient to build their skeletons. Silicon eventually reaches the seafloor, but its journey into the abyss is not straightforward due to biological, physical, and chemical processes. All these processes transport and transform silicon, creating a cycle that we call the marine silicon cycle. The silicon cycle is directly connected to the carbon cycle, making silicon a key player in the regulation of Earth’s climate. In this article, we discuss why we need to understand the marine silicon cycle, explain the steps that happen in the ocean, and demonstrate how the marine silicon cycle affects humans.
All Aboard! Behind the Scenes of a Scientific Research Cruise
Authors: Light Tricia , Norris Emmet , Zhai Dongran , Varner Ruth , Lanpher Kaycie B. , Capone Dante , Erazo Natalia G. , Norris Richard
Published: January 2024

From our climate to the air we breathe, the ocean influences the world around us. Scientists are always looking for new ways to explore and study the ocean. One way we do this is by going on specially designed ships that allow us to study the deep sea, far from land. On our latest expedition aboard the Research Vessel Sally Ride, we went out 300 miles into the North Pacific Ocean for a week. We used some of the most important ocean science tools to catch tiny marine animals, collect water from some of the deepest depths, uncover mysteries of oceans past, and study how desert dust feeds marine animals today.
Easy Or Hard? Basic Questions in Computational Complexity Theory
Authors: Segev Noa , Wigderson Avi
Published: January 2024

Many things in our lives are designed to solve problems. Whether it is an app on our cellphones, the construction of a new building, or the development of a new drug, solving problems is a big motivator. Did you know that there is a fascinating type of mathematics behind many of the complex problems we face in our daily lives? This mathematics is called computational complexity theory, and it is a field of computer science. This is an active, constantly developing field that is attracting many talented young people—like you! In this article, we will describe computational complexity theory and the kinds of problems it is designed to help with. We hope that by the time you finish reading this article, you will be convinced that computational complexity theory is one of the most exciting fields of science.
Oriental melon roots metabolites changing response to the pathogen of Fusarium oxysporum f. sp. melonis mediated by Trichoderma harzianum
Authors: Li Chunxia , Nie Jiahui , Wu Xingbiao , Zhang Yuting , Li Xiang , Wu Xia , Yin Kuide , Jin Yazhong
Published: January 2024

Introduction

Trichoderma spp. is a recognized bio-control agent that promotes plant growth and enhances resistance against soil-borne diseases, especially Fusarium wilt. It is frequently suggested that there is a relationship between resistance to melon wilt and changes in soil microbiome structures in the rhizosphere with plant metabolites. However, the exact mechanism remains unclear.

Method

This study aims to investigate the effects of Trichoderma application on the metabolic pathway of oriental melon roots in response to Fusarium oxysporum f. sp. melonis in a pot experiment. The experiment consisted of three treatments, namely water-treated (CK), FOM-inoculated (KW), and Trichoderma-applied (MM) treatments, that lasted for 25 days. Ultra-performance liquid chromatography-electron spray ionization-mass spectrometry (UPLC-ESI-MS) was used to analyze the compounds in melon roots.

Results

The results show that Trichoderma harzianum application resulted in a reduction in the severity of oriental melon Fusarium wilt. A total of 416 distinct metabolites, categorized into four groups, were detected among the 886 metabolites analyzed. Additionally, seven differential metabolites were identified as key compounds being accumulated after inoculation with Fusarium oxysporum f. sp. melonis (FOM) and Trichoderma. The mechanism by which Trichoderma enhanced melon's resistance to Fusarium wilt was primarily associated with glycolysis/gluconeogenesis, phenylpropanoid biosynthesis, flavone and flavonol biosynthesis, and the biosynthesis of cofactors pathway. In comparison with the treatments of CK and MM, the KW treatment increased the metabolites of flavone and flavonol biosynthesis, suggesting that oriental melon defended against pathogen infection by increasing flavonol biosynthesis in the KW treatment, whereas the application of Trichoderma harzianum decreased pathogen infection while also increasing the biosynthesis of glycolysis/gluconeogenesis and biosynthesis of cofactors pathway, which were related to growth. This study also aims to enhance our understanding of how melon responds to FOM infection and the mechanisms by which Trichoderma harzianum treatment improves melon resistance at the metabolic level.
Mental Health Consultations in Immigration Detention: What Can We Learn From Clinical Records?
Authors: Gonçalves Leonel C. , Heller Patrick , Bachmann Anne-Claire B. , Barbolini Jonathan , Fuhrer Clara , Gétaz Laurent , Luke Eric , Wolff Hans , Baggio Stéphanie
Published: January 2024

Objectives: Knowledge on mental health consultations in immigration detention and characteristics of people receiving consultations is scarce. Based on a sample of 230 adult men in immigration detention in Switzerland, we aimed to: (1) Quantify the proportion of persons receiving mental health consultations during detention; and (2) Identify socio-demographic and clinical characteristics associated with mental health consultations.

Methods: Retrospective observational study with a cross-sectional design. Prevalence estimates, logistic regressions, and contingency tables were used to analyse the data.

Results: A total of 30% of the sample received mental health consultations during detention. Time spent in immigration detention, mental health problems during detention, use of psychotropic medication, and self-harm were associated with mental health consultations. Although mental health consultations are provided to people with more severe mental health problems, 41% of persons with assessed mental health needs during the initial screening and 26% of those who self-harmed during detention did not receive mental health consultations.

Conclusion: Mental health resources and screening procedures could be improved to ensure that mental health consultations are matched to clinical need in immigration detention settings.
Rechallenge of brentuximab vedotin was effective for refractory mycosis fungoides: a case report
Authors: Kawahara Hikaru , Okada Etsuko , Sawada Yu
Published: January 2024

Mycosis fungoides (MF) is a type of primary cutaneous T-cell lymphoma. The anti-cluster of differentiation (CD) 30 antibody agent, brentuximab vedotin (BV), has recently been developed for specific targets against CD30-expressed tumor cells with high efficacy against various lymphomas. Herein, we present a case of marginally CD30-expressed MF successfully treated with BV rechallenge.
Skład parlamentu w Polsce na tle europejskim – przyczynek do dyskusji
Authors: Woźnicki Marek
Published: January 2024

This article will analyse the Polish constitutional regulations on the numer of parliament in comparison with the relevant provisions of the basic laws of the other 26 EU member states, and Great Britain. In particular, a comparison will be made between the Polish proposals to reduce the number of parliamentarians and the regulations adopted in the Italian Constitutional Law of 2019, together with their justification. The analysis of these issues allows the conclusion, that the number of parliamentarians in Poland is relatively high compared to the so-called large European countries. In the conclusions will be presented the proposals for changes to Polish constitutional provisions regarding the number of members of the Sejm.
Teoretyczne rozważania nad nacjonalizmem i jego kondycją w Ukrainie
Authors: Hajduk Jurij
Published: January 2024

Referring to a broad theoretical reflection on the phenomenon of nationalism, the author aims to identify/name the elements that shape contemporary Ukrainian nationalism. By means of an analysis of the program records of national groups, as well as the content of their postulates, together with a list of the level of social support for them, the text attempts to present the condition of contemporary Ukrainian nationalism, which is distinguished not only by its extreme attitudes, but is also a social and political movement for the benefit of the nation. In its radical form, nationalism has never enjoyed much support in Ukrainian society. After Russia’s aggression against Ukraine, it became more of a political nation-building movement that contributed to the emergence of the phenomenon of patriotism, which is not synonymous with ethnonationalism, but refers to its symbols.
О новых полномочиях Конституционного Суда Республики Беларусь
Authors: Пастухов Михаил Иванович
Published: January 2024

The purpose of the article is to analyze the powers of the Constitutional Court of the Republic of Belarus, which were enshrined in the new edition of the Constitution of the Republic of Belarus. In preparing the article, systemic, structural-logical and comparative legal methods were used. As a result, a detailed assessment of the new powers of the Constitutional Court of the Republic of Belarus is given, taking into account the existing practice and experience of the constitutional justice bodies of foreign countries, including the Republic of Poland. The author makes a number of proposals for improving the legislation of the Republic of Belarus.
Citizens in the “Realm of Anti-Politics”
Authors: Tobiasz Marcin
Published: January 2024

The term anti-politics refers to a whole catalog of phenomena such as the decline in citizens’ interest in politics and participation in formal political institutions the process of depoliticization and technocratization of democracy and the rise inpopularity of right-wing populist parties and nationalism. The purpose of this article isto explore what anti-politics really is. This requires not only going beyond an analysis of current politicsbut also a pure descriptivism that simply registers changes in the attitudes of participants in public life. It is necessary to look at this complex phenomenon not only in the long termbut in different interpretive contexts
The Role of Local Government Units in Building Civil Society – the Constructing Civil Society Educational Project (Some Remarks)
Authors: Rachwał Marcin
Published: January 2024

Civil society is an indispensable component of a functional democratic state. The development of civil society is dependent on a number of factors, including the work of the education system. Education is the crucial space for shaping civic attitudes and acquiring competences necessary for a society that is aware of its rights and active in the public sphere. A significant majority of educational institutions is administratively supervised by local government units. The purpose of this paper is to indicate how local authorities can play a role in the shaping of civil society by performing tasks (both obligatory and additional, non-compulsory ones) related to education.
Following the Writer’s Path to the Dynamically Coalescing Reactive Chains Design Pattern
Authors: Oliveira Marum João Paulo , Cunningham H. Conrad , Jones J. Adam , Liu Yi
Published: January 2024

Two recent studies addressed the problem of reducing transitional turbulence in applications developed in C# on .NET. The first study investigated this problem in desktop and Web GUI applications and the second in virtual and augmented reality applications using the Unity3D game engine. The studies used similar solution approaches, but both were somewhat embedded in the details of their applications and implementation platforms. This paper examines these two families of applications and seeks to extract the common aspects of their problem definitions and solution approaches and codify the problem-solution pair as a new software design pattern. To do so, the paper adopts Wellhausen and Fiesser’s writer’s path methodology and follows it systematically to discover and write the pattern, recording the reasoning at each step. To evaluate the pattern, the paper applies it to an arbitrary C#/.NET GUI application. The resulting design pattern is named Dynamically Coalescing Reactive Chains (DCRC). It enables the approach to transitional turbulence reduction to be reused across a range of related applications, languages, and user interface technologies. The detailed example of the writer’s path can assist future pattern writers in navigating through the complications and subtleties of the pattern-writing process.