bluuebunny/crossref_metadata_2025 huggingface dataset:
Regional Diversification of Potential, Production and Efficiency of Use of Biogas and Biomass in Poland
Authors: Ślusarz Grzegorz , Gołębiewska Barbara , Cierpiał-Wolan Marek , Gołębiewski Jarosław , Twaróg Dariusz , Wójcik Sebastian
Published: February 2021

Energy obtained from renewable sources is an important element of the sustainable development strategy of the European Union and its member states. The aim of this research is, therefore, to assess the potential and use of renewable energy sources and their effectiveness from the regional perspective in Poland. The research covered the years 2012 and 2018. The diversification of production and potential of renewable energy sources was defined on the basis of biogas and biomass. Calculations made using the data envelopment analysis (DEA) method showed that, in 2012, only three voivodeships achieved the highest efficiency in terms of the use of biogas and biomass resources; in 2018, this number increased to four. Comparing the effective units in 2012 and 2018, it can be seen that their efficiency frontier moved upwards by 56% in terms of biogas and 21% in terms of to biomass. Despite a large relative increase in the production of heat from biogas by 99% compared to the production of heat from biomass by 38%, the efficiency frontier for biogas did not change considerably. It was found that the resources of solid biomass are used far more intensively than the resources of biogas. However, in the case of biogas, a significant increase in the utilization of the production potential was observed: from 3.3% in 2012 to 6.4% in 2018, whereas in the same years, the utilization of solid biomass production potential remained at the same level (15.3% in 2012, 15.4% in 2018). It was also observed that, at the level of voivodeships, the utilization of biogas and biomass production potential is negatively correlated with the size of this potential. The combined potential of solid biomass and biogas can cover the demand of each of the studied regions in Poland in terms of thermal energy. The coverage ranges from 104% to 1402%. The results show that when comparing biomass and biogas, the production of both electricity and heat was dominated by solid biomass. Its high share occurred especially in voivodeships characterized by a high share of forest area and a low potential for biogas production (Lubuskie Voivodeship, Zachodniopomorskie Voivodeship).
Adaptive Online State of Charge Estimation of EVs Lithium-Ion Batteries with Deep Recurrent Neural Networks
Authors: Javid Gelareh , Ould Abdeslam Djaffar , Basset Michel
Published: February 2021

The State of Charge (SOC) estimation is a significant issue for safe performance and the lifespan of Lithium-ion (Li-ion) batteries. In this paper, a Robust Adaptive Online Long Short-Term Memory (RoLSTM) method is proposed to extract SOC estimation for Li-ion Batteries in Electric Vehicles (EVs). This real-time, as its name suggests, method is based on a Recurrent Neural Network (RNN) containing Long Short-Term Memory (LSTM) units and using the Robust and Adaptive online gradient learning method (RoAdam) for optimization. In the proposed architecture, one sequential model is defined for each of the three inputs: voltage, current, and temperature of the battery. Therefore, the three networks work in parallel. With this approach, the number of LSTM units are reduced. Using this suggested method, one is not dependent on precise battery models and can avoid complicated mathematical methods. In addition, unlike the traditional recursive neural network where content is re-written at any time, the LSTM network can decide on preserving the current memory through the proposed gateways. In that case, it can easily transfer this information over long paths to receive and maintain long-term dependencies. Using real databases, the experiment results illustrate the better performance of RoLSTM applied to SOC estimation of Li-Ion batteries in comparison with a neural network modeling and unscented Kalman filter method that have been used thus far.
Variable-Order Equivalent Circuit Modeling and State of Charge Estimation of Lithium-Ion Battery Based on Electrochemical Impedance Spectroscopy
Authors: Zhang Ji’ang , Wang Ping , Liu Yushu , Cheng Ze
Published: February 2021

In the battery management system, it is important to accurately and efficiently estimate the state of charge (SOC) of lithium-ion batteries, which generally requires the establishment of a equivalent circuit model of the battery, whose accuracy and rationality play an important role in accurately estimating the state of lithium-ion batteries. The traditional single order equivalent circuit models do not take into account the changes of impedance spectrum under the action of multiple factors, nor do they take into account the balance of practicality and complexity of the model, resulting the low accuracy and poor practicability. In this paper, the theory of electrochemical impedance spectroscopy is used to guide and improve the equivalent circuit model. Based on the analysis of the variation of the high and intermediate frequency range of the impedance spectrum with the state of charge and temperature of the battery, a variable order equivalent model (VOEM) is proposed by Arrhenius equation and Bayesian information criterion (BIC), and the state equation and observation equation of VOEM are improved by autoregressive (AR) equations. Combined with the unscented Kalman filter (UKF), a SOC online estimation method is proposed, named VOEM-AR-UKF. The experimental results show that the proposed method has high accuracy and good adaptability.
How High Is the Recreation Value of Successional Forests Growing Spontaneously on Coal Mine Spoil Heaps?
Authors: Braun Kohlová Markéta , Máca Vojtěch , Melichar Jan , Pavelčík Petr
Published: January 2021

Research highlights: Recreation value increases with the age of replanted as well as successional forests. Successional forests are not systematically less valuable for recreation than replanted forests. Succession may be used as a viable and low-cost reclamation practice of spoil heaps. Background and objectives: Afforestation has been a popular practice in post-mining land reclamation in the Czech Republic. To expand the current evidence on the recreation values of reclaimed forests, we conducted a valuation study for most typical reclaimed forests, as well as for successional forests spontaneously growing on surface coal mine deposit heaps. Using two distinct measurement methods, we also explore whether the estimated recreation value of forests is robust. Materials and Methods: An online survey was conducted in 2016 on a sample of residents living in the vicinity of coal mine deposit heaps, residents of the adjacent region of Karlovy Vary, and a control population from the central Bohemian region. Participants evaluated visual representations of forest stands typical for reclamation and succession, along with commercial spruce forest as a reference type. In the direct measurement, we measured the attractiveness of a respective forest for a walk using a 5-point scale. In the indirect measurement, a hypothetical choice between two forests for a walk was elicited in a discrete choice experiment. Results: Both direct and indirect measurements provide similar results. All reclaimed forests have a lower recreation value than the reference spruce forest. Successional forests are not systematically less valuable for recreation than replanted forests and the recreation value of both types increases with their age. The age, gender, and education of the participants did not affect the recreation value of a forest. Conclusions: We demonstrate that succession may be used as a viable and low-cost reclamation practice of spoil heaps emerging as a by-product of open-cast coal mining. With recreation as only one of many forest uses, our findings need to be interpreted vis-à-vis the objectives and expected results for individual sites and their habitat conditions.
The Populations and Habitat Preferences of Three Elm Species in Conditions Prevailing on Plains of Poland
Authors: Napierała-Filipiak Anna , Filipiak Maciej , Jaworek-Jakubska Justyna
Published: January 2021

From among the 35–40 elm species existing around the world, three are native to Europe: the wych elm (Ulmus glabra Huds.), the European white elm, (Ulmus laevis Pall.), and the field elm (Ulmus minor Mill.). The populations of these trees have been greatly reduced as a result of a decrease in the total area of riparian forests. Furthermore, for nearly 100 years, they have been destroyed by Dutch elm disease (DED). The main research problems of this study are: what are the habitat preferences of elms growing in a given area; and whether the occurrence of DED depends on the species of elm and the habitat in which it occurs. The results presented here are based on field studies and observations have been supplemented with data from forest inventories. All of the examined elms are definitely more abundant in habitats that are fertile or very fertile and moist, with a neutral or slightly alkaline soil pH. The preference for moist sites is the most evident in the case of U. laevis and the least evident in the case of U. glabra. A slight shift in habitat preferences of the field elm, compared to the white elm, towards less humid conditions was observed. The predominant species of elm in the studied area is currently U. laevis, which was rarely cultivated in forests in the past. In the examined area, the field elm population is clearly on the decline mainly due to the long-term presence of Dutch elm disease. U. glabra is the rarest species in the examined area and most often found on slopes. The current proportions of individual species should be maintained. This paper discusses factors, including ones not connected with DED, that may be responsible for the current state of populations of particular species of elm in forests of Central Europe.
Ablation of SMUG1 Reduces Cell Viability and Increases UVC-Mediated Apoptosis in Hepatocarcinoma HepG2 Cells
Authors: An Mi-Jin , Shin Geun-Seup , Lee Hyun-Min , Kim Jung-Woong
Published: January 2021

Uracil is an unavoidable aberrant base in DNA sequences, the repair of which takes place by a highly efficient base excision repair mechanism. The removal of uracil from the genome requires multiple biochemical steps with conformational changes of DNA that inhibit DNA replication and interfere with transcription. However, the relevance of uracil in DNA for cellular physiology and transcriptional regulation is not fully understood. We investigated the functional roles of SMUG1 using knock-down (KD) and knock-out (KO) models. The proliferation ratio of SMUG1 KD and KO cells was decreased compared to WT control cells, and the cell cycle was arrested in the G2/M phases before the transition to mitosis. The apoptotic cell death was increased in KD and KO cell lines through the increase of BAX and active caspase 3 expression. Phospho-gamma-H2AX expression, which reflected accumulated DNA damage, was also increased in KO cells. Moreover, the apoptotic cells by DNA damage accumulation were markedly increased in SMUG1 KD and KO cells after ultraviolet C irradiation. Transcriptomic analysis using RNA-seq revealed that SMUG1 was involved in gene sets expression including cell cycle transition and chromatin silencing. Together, the results implicate SMUG1 as a critical factor in cell cycle and transcriptional regulation.
Release Mechanism, Secondary Pollutants and Denitrification Performance Comparison of Six Kinds of Agricultural Wastes as Solid Carbon Sources for Nitrate Removal
Authors: Ling Yu , Yan Guokai , Wang Haiyan , Dong Weiyang , Wang Huan , Chang Yang , Chang Ming , Li Congyu
Published: January 2021

Agricultural wastes used as denitrification carbon sources have some drawbacks such as excessive organic carbon release and unclear release characteristics of nitrogen, phosphorus, and chromatic substances, which can cause adverse effects on the effluent quality during the denitrification process. The composition and surface characteristics, carbon release mechanisms, and secondary pollutant release properties of six kinds of agricultural wastes, i.e., rice straw (RS), wheat straw (WS), corn stalk (CS), corncob (CC), soybean stalk (SS), and soybean hull (SH) were studied and analyzed in this research. The denitrification performance of these agricultural wastes was also investigated extensively by batch experiments. The results showed that the carbon release basically followed the second-order reaction kinetic equation and Ritger–Peppas equation in the 120 h reaction, and it was mainly controlled by the diffusion process. The kinetic equation fitting results and bioavailability test suggested that the potential risk of excessive effluent COD of CC was the lowest due to the appropriate amount and degradability of its released carbon. The NH4+-N, TN, and TP in the leachate of RS were higher than those of the other five agriculture wastes, and the chroma in the leachate of WS and CS was heavier than that of the others. CC released the lowest pollutants, which resulted in slight fluctuations of effluent quality in the start-up period (1–11 d), and it had the best nitrogen removal capacity in the denitrification experiment. The average NO3−-N removal of CC was 5.12 mg for each batch in the stable period (11–27 d), which was higher than that of others, and less NO2−-N, NH4+-N, and COD were accumulated in the CC effluent during the whole denitrification process.
Effects of General Physical Activity Promoting Interventions on Functional Outcomes in Patients Hospitalized over 48 Hours: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Seeger Joost P. H. , Koenders Niek , Staal J. Bart , Hoogeboom Thomas J.
Published: January 2021

Low physical activity of patients is a global problem and associated with loss of strength and independent mobility. This study analyzes the effect of general physical activity promoting interventions on functional and hospital outcomes in patients hospitalized over 48 h. Five electronic databases were searched for randomized controlled trials. For outcomes reported in two studies or more, a meta-analysis was performed to test between-group differences (intervention versus control) using a random-effects model. The Grading of Recommendations Assessment, Development, and Evaluation (GRADE) approach was used to rate the certainty of evidence for each outcome. Out of 23,302 identified studies, we included four studies (in total n = 368 participants). We found with moderate certainty of evidence 0 reported falls in the intervention (n = 126) versus five reported falls in the control (n = 122), a non-statistically significant difference between intervention and control groups (p = 0.06). In addition, we found with (very) low certainty of evidence no statistically significant differences between groups on activities of daily living (ADL-activity) and time spent standing and walking. Overall, we found no conclusive evidence on the effect of general physical activity promoting interventions on functional outcomes. More research is needed to understand and improve the effect of general physical activity promoting interventions for patients during the hospital stay.
Adenomyosis and Infertility—Review of Medical and Surgical Approaches
Authors: Szubert Maria , Koziróg Edward , Olszak Olga , Krygier-Kurz Klaudia , Kazmierczak Jakub , Wilczynski Jacek
Published: January 2021

The aim of this review is to clarify the relative association between adenomyosis and infertility and the possible treatment for an infertile patient. Although adenomyosis is detected more often in women of late reproductive age, its influence on pregnancy rates is important, especially considering the tendency to delay pregnancy among women in developed countries. In this article, we present a critical analysis of the literature data concerning the impact of adenomyosis on fertility. The possible effects of treatment on the pregnancy rate will also be discussed. We conducted a literature search; publications from Pubmed, Embase and Cochrane databases published from 1982 to 2019 were retrieved using terms ’adenomyosis and infertility’ and ’adenomyosis and pregnancy outcomes’, extensively studied in the aspects of diagnosis, pathogenesis of infertility and possible treatment methods. Molecular studies have given deep insight into the pathogenesis of adenomyosis in the recent few years, but there is a huge discrepancy between in vitro studies and praxis. Oral contraceptive pills, anti-prostaglandins, oral or parenteral progestins, danazol and gonadotrophin-releasing hormone (GnRH) analogues have all been used to control menstrual pain and menorrhagia in women with adenomyosis, but they temporarily suppress the menstrual cycle. Additionally, endometrial ablation and hysterectomy used to alleviate pain caused by adenomyosis exclude pregnancy planning. The development of imaging techniques—ultrasound and MRI—enables the diagnosis of adenomyosis with very high accuracy nowadays, but the methods of treatment mentioned above have not given satisfactory results in women planning pregnancy. For these patients, the high-intensity-focused ultrasound method (HIFU) and combined treatment before assisted reproductive techniques can prove beneficial in adenomyosis patients.
Swimming World Championships: Association between Success at the Junior and Senior Level for British Swimmers
Authors: Yustres Inmaculada , del Cerro Jesús Santos , Psycharakis Stelios , González-Mohíno Fernando , González-Ravé José María
Published: January 2021

The present study examines the association between the success at junior and senior level for British swimmers in World Championships (WCs). It also explores the relationships between swimming performance and the swimmers’ gender, swim stroke, distance, status (finalist, semifinalist and heats) and swimming category. Data were collected for swimmers participating in junior and/or senior World Championships (29,000 entries: 5585 swimmers) from 2006–2017. The final filtered database included only swimmers from the United Kingdom (836 entries: 141 swimmers). A descriptive analysis was made to characterize the swimmers who reached elite status in the senior category. A lineal regression model was run by gender to predict the influence of category, swim stroke, and distance in the results reached in the senior category. The results showed that the ratio of conversion from junior to senior was quite low. Females who participated in both junior and senior WCs were likely to reach top positions in the senior category. Overall, few British swimmers participated in a junior category before the senior level, but female swimmers participating in both junior and senior WCs were likely to reach top positions in the senior category.
Locations of Physical Activity: Where Are Children, Adolescents, and Adults Physically Active? A Systematic Review
Authors: Kelso Anne , Reimers Anne K , Abu-Omar Karim , Wunsch Kathrin , Niessner Claudia , Wäsche Hagen , Demetriou Yolanda
Published: January 2021

The aim of this systematic review was to examine where physical activity (PA) takes place and how much time children, adolescents and adults spend being physically active within the identified locations. A systematic literature search was carried out in five electronic databases (PubMed, CINAHL, SPORTDiscus, PsycInfo, Scopus). For inclusion, primary studies had to identify locations of PA using device-based or self-report tools, whereas minutes of PA had to be examined using device-based tools only. Thirty-two studies were included, methodological quality and sex/gender sensitivity of the studies were assessed. The narrative data synthesis revealed that the highest average amount of daily moderate-to-vigorous PA was found in home and recreational locations, followed by school and neighborhood locations. In adults, highest average amount of daily moderate-to-vigorous PA was found in neighborhood and home locations followed by workplace and recreational locations. The majority of studies had a low risk of bias in four out of six domains; eight studies reported significant sex/gender differences in location-based PA. The results indicate that different locations are used for PA to a varying degree across the lifespan. Future research on the promotion of PA should focus on location-specific design features that encourage children, adolescents and adults to be physically active.
Sirt1 Activity in the Brain: Simultaneous Effects on Energy Homeostasis and Reproduction
Authors: D’Angelo Stefania , Mele Elena , Di Filippo Federico , Viggiano Andrea , Meccariello Rosaria
Published: January 2021

Diet deeply impacts brain functions like synaptic plasticity and cognitive processes, neuroendocrine functions, reproduction and behaviour, with detrimental or protective effects on neuronal physiology and therefore consequences for health. In this respect, the activity of metabolic sensors within the brain is critical for the maintenance of health status and represents a possible therapeutic target for some diseases. This review summarizes the main activity of Sirtuin1 (Sirt1), a metabolic sensor within the brain with a focus on the link between the central control of energy homeostasis and reproduction. The possible modulation of Sirt1 by natural phytochemical compounds like polyphenols is also discussed.
Pathways among Frailty, Health Literacy, Acculturation, and Social Support of Middle-Aged and Older Korean Immigrants in the USA
Authors: Sagong Hae , Yoon Ju Young
Published: January 2021

Culturally and linguistically different immigrants in the U.S. are considered populations with low health literacy in general, thereby having a high risk of negative health outcomes such as frailty. The purpose of this study is to identify the effects of social support and acculturation on the relationship between health literacy and frailty of Korean immigrants in existing models of health literacy. A total of 244 Korean immigrants aged 50 years and older residing in Southern United States (Alabama and Georgia) were recruited. Path analysis was used to examine the pathways among variables, and the indirect effects of health literacy were analyzed. The results revealed that health literacy and social support directly influenced frailty; social support and acculturation were identified to influence health literacy. Health literacy had a partial mediating effect in the relationship between social support and frailty and a complete mediating effect in the relationship between acculturation and frailty. Therefore, to prevent frailty, it is necessary to consider enhancing immigrants’ health literacy by elevating acculturation and social supports.
Cross-Cultural Adaptation and Validation of the Brazilian Portuguese Version of an Observational Measure for Parent–Child Responsive Caregiving
Authors: Schneider Alessandra , Rodrigues Michelle , Falenchuk Olesya , Munhoz Tiago N. , Barros Aluisio J. D. , Murray Joseph , Domingues Marlos R. , Jenkins Jennifer M.
Published: January 2021

Responsive caregiving is the dimension of parenting most consistently related to later child functioning in both developing and developed countries. There is a growing need for efficient, psychometrically sound and culturally appropriate measurement of this construct. This study describes the cross-cultural validation in Brazil of the Responsive Interactions for Learning (RIFL-P) measure, requiring only eight minutes for assessment and coding. The cross-cultural adaptation used a recognized seven-step procedure. The adapted version was applied to a stratified sample of 153 Brazilian mother–child (18 months) dyads. Videos of mother–child interaction were coded using the RIFL-P and a longer gold standard parenting assessment. Mothers completed a survey on child stimulation (18 months) and child outcomes were measured at 24 months. Internal consistency (α = 0.94), inter-rater reliability (r = 0.83), and intra-rater reliability (r = 0.94) were all satisfactory to high. RIFL-P scores were significantly correlated with another measurement of parenting (r’s ranged from 0.32 to 0.47, p < 0.001), stimulation markers (r = 0.34, p < 0.01), and children’s cognition (r = 0.29, p < 0.001), language (r = 0.28, p < 0.001), and positive behavior (r = 0.17, p < 0.05). The Brazilian Portuguese version is a valid and reliable instrument for a brief assessment of responsive caregiving.
Targeting Gut Microbiota to Treat Hypertension: A Systematic Review
Authors: Palmu Joonatan , Lahti Leo , Niiranen Teemu
Published: January 2021

While hypertension remains the leading modifiable risk factor for cardiovascular morbidity and mortality, the pathogenesis of essential hypertension remains only partially understood. Recently, microbial dysbiosis has been associated with multiple chronic diseases closely related to hypertension. In addition, multiple small-scale animal and human studies have provided promising results for the association between gut microbial dysbiosis and hypertension. Animal models and a small human pilot study, have demonstrated that high salt intake, a risk factor for both hypertension and cardiovascular disease, depletes certain Lactobacillus species while oral treatment of Lactobacilli prevented salt-sensitive hypertension. To date, four large cohort studies have reported modest associations between gut microbiota features and hypertension. In this systematic literature review, we examine the previously reported links between the gut microbiota and hypertension and what is known about the functional mechanisms behind this association.
Sleep Status and the Associated Factors: A Large Cross-Sectional Study in Shaanxi Province, China
Authors: Zhang Yaxuan , Wang Jiwei , Lu Xinyuan , Che Beibei , Yu Jinming
Published: January 2021

This study aimed at investigating the sleep status and its associated factors in Shaanxi province, China. We conducted a cross-sectional study among 11,399 subjects in Shaanxi Province, China. Data were collected via spot field questionnaire surveys. The contents included demographic characteristics, sleep status, lifestyles, disease history and other associated factors. Logistic regression analysis was used to estimate the effect of associated factors on sleep quality. A total of 11,036 subjects were included in the final analysis. In total, 12.8% of the participants had bad or very bad sleep. In the last month, 8.4% of the participants had difficulty in initiating sleep, 7.6% of the participants had difficulty in maintaining sleep, 8.8% of the participants suffered from awakening earlier and 10.3% of the participants had the problem of feeling sleepy during the day ≥3 times per week. Poorer sleep quality was associated with being female, being unmarried or without cohabiting with a boyfriend/girlfriend, being divorced or widowed, heart diseases, musculoskeletal diseases, concerns about their own health, drinking alcohol, taking hypnotics, and a longer daily screen time. Better sleep quality was associated with medium education level, high family monthly income, good self-reported health status, and having breakfast regularly. In conclusion, more than one in ten people did not sleep well and suffered from different sleep problems in Shaanxi, China. Sleep quality was associated with sex, marital status, educational level, family monthly income, heart disease, musculoskeletal diseases, degree of concerning about their own health, self-reported health status, drinking alcohol, having breakfast, taking hypnotics and daily screen time.
End-of-Life Care Mobile App for Intensive-Care Unit Nurses: A Quasi-Experimental Study
Authors: Yang Jin Hee , Shin Gisoo
Published: January 2021

Intensive-care unit nurses may experience difficulties in end-of-life care because of frustration or lethargy. The purpose of this study was to develop a mobile end-of-life care program for intensive-care unit nurses and evaluate the effects on competence factors such as knowledge, self-efficacy, and compassion. A quasi-experimental design was used. The participants included 44 nurses who had less than three years of experience in the intensive-care unit, divided into the experimental group and control group. After the intervention, the experimental group showed a significant improvement in self-efficacy in end-of-life care and compassion in end-of-life care. Based on the results of this study, the end-of-life care mobile app was an effective educational method for nurses with experience of less than 3 years in an intensive-care unit. To improve the quality of end-of-life care, it is necessary to develop various educational programs considering the greater role of the fourth industrial revolution in the future.
Influences of Recreational Tennis-Playing Exercise Time on Cardiometabolic Health Parameters in Healthy Elderly: The ExAMIN AGE Study
Authors: Chao Hsiao-Han , Liao Yi-Hung , Chou Chun-Chung
Published: January 2021

Background: Aging and chronic degeneration are the primary threats to cardiometabolic health in elderly populations. Regular appropriate exercise would benefit the advanced aging population. Purpose: This study investigates whether the degree of weekly tennis participation exhibits differences in primary cardiometabolic parameters, including arterial stiffness, inflammation, and metabolic biomarkers in elderly tennis players. Methods: One hundred thirty-five long-term participants in elder tennis (>50 years old) were initially screened. Twenty-six eligible and voluntary subjects were divided into high tennis time group (HT) (14 ± 1.3 h/week) and low tennis time group (LT) (4.5 ± 0.7 h/week) by stratification analysis based on the amount of tennis playing activity time. The brachial-ankle pulse wave velocity (baPWV), blood pressure, ankle-brachial index (ABI), blood metabolic biomarkers, and insulin resistance were measured to compare the difference between HT and LT groups. Results: The baPWV was significantly lower in the HT group than that in the LT group (1283.92 ± 37.01 vs. 1403.69 ± 53.71 cm/s, p < 0.05). We also found that the HT insulin-resistant homeostasis model assessment (HOMA-IR) was significantly lower than that of LT (1.41 ± 0.11 vs. 2.27 ± 0.48 μIU/mL, p < 0.05). However, the blood lipid biomarkers (glucose, cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglyceride) were not statistical different between HT and LT groups (p > 0.05). Conclusion: We demonstrated that under the condition of similar daily physical activity level, elderly with a higher time of tennis-playing (HT group) exhibited relatively lower arterial stiffness (lower PWV) and lower insulin resistance compared to those with lower time tennis-playing (LT).
DEM Based Study on Shielded Astronomical Solar Radiation and Possible Sunshine Duration under Terrain Influences on Mars by Using Spectral Methods
Authors: Lin Siwei , Chen Nan
Published: January 2021

Solar radiation may be shielded by the terrain relief before reaching the Martian surface, especially over some rugged terrains. Yet, to date, no comprehensive studies on the spatial structure of shielded astronomical solar radiation (SASR) and the possible sunshine duration (PSD) on Mars have been conducted by previous researchers. Previous studies generally ignored the influences of the terrain on the SASR and PSD, which resulted in a corresponding unexplored field on SASR. The purpose of this paper is to study the Martian spatial-temporal structure of SASR and the PSD under terrain influences. In this paper, the theory of Earth’s SASR, the previous Martian SASR model and the theory of planetary science were combined to propose the SASR model that can be applied to Mars. Then, with the spectrum method theory of geography, we defined two new concepts of spectrums to explore the spatial-temporal distribution of SASR and PSD in different Martian landforms. We found SASR and PSD on Mars were significantly influenced by terrain relief and latitude and showed sufficient regularity, which can be concluded as a gradual attenuation with terrain relief and a regularity of latitude anisotropy. The latitude anisotropy feature is a manifestation of the terrain shielding effect. With the latitude varying, SASR and PSD at different temporal scale generally showed different features with those of Earth, which may be attributed to the imbalanced seasons caused by Martian moving orbits and velocity. Compared to PSD, SASR showed more regular variation under terrain relief and was more influenced by the terrain relief which revealed that SASR is more sensitive to terrain relief than PSD. Additionally, the critical area is a quantitative index to reflect the stable spatial structure of SASR and PSD in different landforms and may be viewed as the minimum test region of sample areas. The corresponding result of the experiments herein indicated that either spectrum can effectively depict the spatial-temporal distribution of SASR and PSD on Mars under terrain relief and deepen the understanding of the variation of SASR and PSD influences by terrain. The critical area of either spectrum can be employed to explore and determine the stable spatial structure of SASR and PSD in different landforms. The proposed Martian SASR model and the new spectral method theory shed new light on revealing the spatial-temporal structure of SASR and PSD under terrain influences on Mars.
Extracellular Vesicles Do Not Mediate the Anti-Inflammatory Actions of Mouse-Derived Adipose Tissue Mesenchymal Stem Cells Secretome
Authors: Carceller María Carmen , Guillén María Isabel , Gil María Luisa , Alcaraz María José
Published: January 2021

Adipose tissue represents an abundant source of mesenchymal stem cells (MSC) for therapeutic purposes. Previous studies have demonstrated the anti-inflammatory potential of adipose tissue-derived MSC (ASC). Extracellular vesicles (EV) present in the conditioned medium (CM) have been shown to mediate the cytoprotective effects of human ASC secretome. Nevertheless, the role of EV in the anti-inflammatory effects of mouse-derived ASC is not known. The current study has investigated the influence of mouse-derived ASC CM and its fractions on the response of mouse-derived peritoneal macrophages against lipopolysaccharide (LPS). CM and its soluble fraction reduced the release of pro-inflammatory cytokines, adenosine triphosphate and nitric oxide in stimulated cells. They also enhanced the migration of neutrophils or monocytes, in the absence or presence of LPS, respectively, which is likely related to the presence of chemokines, and reduced the phagocytic response. The anti-inflammatory effect of CM may be dependent on the regulation of toll-like receptor 4 expression and nuclear factor-κB activation. Our results demonstrate the anti-inflammatory effects of mouse-derived ASC secretome in mouse-derived peritoneal macrophages stimulated with LPS and show that they are not mediated by EV.
Impairment of Spike-Timing-Dependent Plasticity at Schaffer Collateral-CA1 Synapses in Adult APP/PS1 Mice Depends on Proximity of Aβ Plaques
Authors: Garad Machhindra , Edelmann Elke , Leßmann Volkmar
Published: January 2021

Alzheimer’s disease (AD) is a multifaceted neurodegenerative disorder characterized by progressive and irreversible cognitive decline, with no disease-modifying therapy until today. Spike timing-dependent plasticity (STDP) is a Hebbian form of synaptic plasticity, and a strong candidate to underlie learning and memory at the single neuron level. Although several studies reported impaired long-term potentiation (LTP) in the hippocampus in AD mouse models, the impact of amyloid-β (Aβ) pathology on STDP in the hippocampus is not known. Using whole cell patch clamp recordings in CA1 pyramidal neurons of acute transversal hippocampal slices, we investigated timing-dependent (t-) LTP induced by STDP paradigms at Schaffer collateral (SC)-CA1 synapses in slices of 6-month-old adult APP/PS1 AD model mice. Our results show that t-LTP can be induced even in fully developed adult mice with different and even low repeat STDP paradigms. Further, adult APP/PS1 mice displayed intact t-LTP induced by 1 presynaptic EPSP paired with 4 postsynaptic APs (6× 1:4) or 1 presynaptic EPSP paired with 1 postsynaptic AP (100× 1:1) STDP paradigms when the position of Aβ plaques relative to recorded CA1 neurons in the slice were not considered. However, when Aβ plaques were live stained with the fluorescent dye methoxy-X04, we observed that in CA1 neurons with their somata <200 µm away from the border of the nearest Aβ plaque, t-LTP induced by 6× 1:4 stimulation was significantly impaired, while t-LTP was unaltered in CA1 neurons >200 µm away from plaques. Treatment of APP/PS1 mice with the anti-inflammatory drug fingolimod that we previously showed to alleviate synaptic deficits in this AD mouse model did not rescue the impaired t-LTP. Our data reveal that overexpression of APP and PS1 mutations in AD model mice disrupts t-LTP in an Aβ plaque distance-dependent manner, but cannot be improved by fingolimod (FTY720) that has been shown to rescue conventional LTP in CA1 of APP/PS1 mice.
Hyperglycemic Condition Causes Pro-Inflammatory and Permeability Alterations Associated with Monocyte Recruitment and Deregulated NFκB/PPARγ Pathways on Cerebral Endothelial Cells: Evidence for Polyphenols Uptake and Protective Effect
Authors: Taïlé Janice , Patché Jessica , Veeren Bryan , Gonthier Marie-Paule
Published: January 2021

Hyperglycemia alters the function of cerebral endothelial cells from the blood-brain barrier, increasing the risk of cerebrovascular complications during diabetes. This study evaluated the protective effect of polyphenols on inflammatory and permeability markers on bEnd3 cerebral endothelial cells exposed to high glucose concentration. Results show that hyperglycemic condition increased nuclear factor kappa B (NFκB) activity, deregulated the expression of interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), interleukin-10 (IL-10) and endothelial-leukocyte adhesion molecule (E-selectin) genes, raised MCP-1 secretion and elevated monocyte adhesion and transendothelial migration. High glucose decreased occludin, claudin-5, zona occludens-1 (ZO-1) and zona occludens-2 (ZO-2) tight junctions production and altered the endothelial permeability. Characterized polyphenolic extracts from the French medicinal plants Antirhea borbonica, Ayapana triplinervis, Dodonaea viscosa and Terminalia bentzoe, and their major polyphenols quercetin, caffeic, chlorogenic and gallic acids limited the pro-inflammatory and permeability alterations caused by high glucose. Peroxisome proliferator-activated receptor gamma (PPARγ) agonist also attenuated these damages while PPARγ antagonist aggravated them, suggesting PPARγ protective action. Interestingly, polyphenols improved PPARγ gene expression lowered by high glucose. Moreover, polyphenols were detected at the intracellular level or membrane-bound to cells, with evidence for breast cancer resistance protein (BCRP) efflux transporter role. Altogether, these findings emphasize the ability of polyphenols to protect cerebral endothelial cells in hyperglycemic condition and their relevance for pharmacological strategies aiming to limit cerebrovascular disorders in diabetes.
Equilibrative Nucleoside Transporters Mediate the Import of Nicotinamide Riboside and Nicotinic Acid Riboside into Human Cells
Authors: Kropotov Andrey , Kulikova Veronika , Nerinovski Kirill , Yakimov Alexander , Svetlova Maria , Solovjeva Ljudmila , Sudnitsyna Julia , Migaud Marie E. , Khodorkovskiy Mikhail , Ziegler Mathias , Nikiforov Andrey
Published: January 2021

Nicotinamide riboside (NR), a new form of vitamin B3, is an effective precursor of nicotinamide adenine dinucleotide (NAD+) in human and animal cells. The introduction of NR into the body effectively increases the level of intracellular NAD+ and thereby restores physiological functions that are weakened or lost in experimental models of aging and various pathologies. Despite the active use of NR in applied biomedicine, the mechanism of its transport into mammalian cells is currently not understood. In this study, we used overexpression of proteins in HEK293 cells, and metabolite detection by NMR, to show that extracellular NR can be imported into cells by members of the equilibrative nucleoside transporter (ENT) family ENT1, ENT2, and ENT4. After being imported into cells, NR is readily metabolized resulting in Nam generation. Moreover, the same ENT-dependent mechanism can be used to import the deamidated form of NR, nicotinic acid riboside (NAR). However, NAR uptake into HEK293 cells required the stimulation of its active utilization in the cytosol such as phosphorylation by NR kinase. On the other hand, we did not detect any NR uptake mediated by the concentrative nucleoside transporters (CNT) CNT1, CNT2, or CNT3, while overexpression of CNT3, but not CNT1 or CNT2, moderately stimulated NAR utilization by HEK293 cells.
Modeling and Molecular Dynamics of the 3D Structure of the HPV16 E7 Protein and Its Variants
Authors: Bello-Rios Ciresthel , Montaño Sarita , Garibay-Cerdenares Olga Lilia , Araujo-Arcos Lilian Esmeralda , Leyva-Vázquez Marco Antonio , Illades-Aguiar Berenice
Published: January 2021

The oncogenic potential of high-risk human papillomavirus (HPV) is predicated on the production of the E6 and E7 oncoproteins, which are responsible for disrupting the control of the cell cycle. Epidemiological studies have proposed that the presence of the N29S and H51N variants of the HPV16 E7 protein is significantly associated with cervical cancer. It has been suggested that changes in the amino acid sequence of E7 variants may affect the oncoprotein 3D structure; however, this remains uncertain. An analysis of the structural differences of the HPV16 E7 protein and its variants (N29S and H51N) was performed through homology modeling and structural refinement by molecular dynamics simulation. We propose, for the first time, a 3D structure of the E7 reference protein and two of Its variants (N29S and H51N), and conclude that the mutations induced by the variants in N29S and H51N have a significant influence on the 3D structure of the E7 protein of HPV16, which could be related to the oncogenic capacity of this protein.
Current Achievements and Applications of Transcriptomics in Personalized Cancer Medicine
Authors: Supplitt Stanislaw , Karpinski Pawel , Sasiadek Maria , Laczmanska Izabela
Published: February 2021

Over the last decades, transcriptome profiling emerged as one of the most powerful approaches in oncology, providing prognostic and predictive utility for cancer management. The development of novel technologies, such as revolutionary next-generation sequencing, enables the identification of cancer biomarkers, gene signatures, and their aberrant expression affecting oncogenesis, as well as the discovery of molecular targets for anticancer therapies. Transcriptomics contribute to a change in the holistic understanding of cancer, from histopathological and organic to molecular classifications, opening a more personalized perspective for tumor diagnostics and therapy. The further advancement on transcriptome profiling may allow standardization and cost reduction of its analysis, which will be the next step for transcriptomics to become a canon of contemporary cancer medicine.
p-Coumaric Acid Enhances Hypothalamic Leptin Signaling and Glucose Homeostasis in Mice via Differential Effects on AMPK Activation
Authors: Nguyen Linh V. , Nguyen Khoa D. A. , Ma Chi-Thanh , Nguyen Quoc-Thai , Nguyen Huong T. H. , Yang Dong-Joo , Tran Trung Le , Kim Ki Woo , Doan Khanh V.
Published: February 2021

AMP-activated protein kinase (AMPK) plays a crucial role in the regulation of energy homeostasis in both peripheral metabolic organs and the central nervous system. Recent studies indicated that p-Coumaric acid (CA), a hydroxycinnamic phenolic acid, potentially activated the peripheral AMPK pathway to exert beneficial effects on glucose metabolism in vitro. However, CA’s actions on central AMPK activity and whole-body glucose homeostasis have not yet been investigated. Here, we reported that CA exhibited different effects on peripheral and central AMPK activation both in vitro and in vivo. Specifically, while CA treatment promoted hepatic AMPK activation, it showed an inhibitory effect on hypothalamic AMPK activity possibly by activating the S6 kinase. Furthermore, CA treatment enhanced hypothalamic leptin sensitivity, resulting in increased proopiomelanocortin (POMC) expression, decreased agouti-related peptide (AgRP) expression, and reduced daily food intake. Overall, CA treatment improved blood glucose control, glucose tolerance, and insulin sensitivity. Together, these results suggested that CA treatment enhanced hypothalamic leptin signaling and whole-body glucose homeostasis, possibly via its differential effects on AMPK activation.
Adipokines and Metabolic Regulators in Human and Experimental Pulmonary Arterial Hypertension
Authors: Papathanasiou Aimilia , Spyropoulos Fotios , Michael Zoe , Joung Kyoung , Briana Despina , Malamitsi-Puchner Ariadne , Mantzoros Christos , Christou Helen
Published: February 2021

Pulmonary hypertension (PH) is associated with meta-inflammation related to obesity but the role of adipose tissue in PH pathogenesis is unknown. We hypothesized that adipose tissue-derived metabolic regulators are altered in human and experimental PH. We measured circulating levels of fatty acid binding protein 4 (FABP-4), fibroblast growth factor -21 (FGF-21), adiponectin, and the mRNA levels of FABP-4, FGF-21, and peroxisome proliferator-activated receptor γ (PPARγ) in lung tissue of patients with idiopathic PH and healthy controls. We also evaluated lung and adipose tissue expression of these mediators in the three most commonly used experimental rodent models of pulmonary hypertension. Circulating levels of FABP-4, FGF-21, and adiponectin were significantly elevated in PH patients compared to controls and the mRNA levels of these regulators and PPARγ were also significantly increased in human PH lungs and in the lungs of rats with experimental PH compared to controls. These findings were coupled with increased levels of adipose tissue mRNA of genes related to glucose uptake, glycolysis, tricarboxylic acid cycle, and fatty acid oxidation in experimental PH. Our results support that metabolic alterations in human PH are recapitulated in rodent models of the disease and suggest that adipose tissue may contribute to PH pathogenesis.
Graphene-Doped Poly (Methyl-Methacrylate) (Pmma) Implants: A Micro-CT and Histomorphometrical Study in Rabbits
Authors: Scarano Antonio , Orsini Tiziana , Di Carlo Fabio , Valbonetti Luca , Lorusso Felice
Published: February 2021

Background—the graphene-doping procedure represents a useful procedure to improve the mechanical, physical and biological response of several Polymethyl methacrylate (PMMA)-derived polymers and biomaterials for dental applications. The aim of this study was to evaluate osseointegration of Graphene doped Poly(methyl methacrylate) (GD-PMMA) compared with PMMA as potential materials for dental implant devices. Methods—eighteen adult New Zealand white male rabbits with a mean weight of approx. 3000 g were used in this research. A total of eighteen implants of 3.5 mm diameter and 11 mm length in GD-PMMA and eighteen implants in PMMA were used. The implants were placed into the articular femoral knee joint. The animals were sacrificed after 15, 30 and 60 days and the specimens were evaluated by µCT and histomorphometry. Results—microscopically, all 36 implants, 18 in PMMA and 18 in DG-PMMA were well-integrated into the bone. The implants were in contact with cortical bone along the upper threads, while the lower threads were in contact with either newly formed bone or with marrow spaces. The histomorphometry and µCT evaluation showed that the GP-PMMA and PMMA implants were well osseointegrated and the bone was in direct contact with large portions of the implant surfaces, including the space in the medullary canal. Conclusions—in conclusion, the results suggest that GD-PMMA titanium surfaces enhance osseointegration in rabbit femurs. This encourages further research to obtain GD-PMMA with a greater radiopacity. Also, further in vitro and vivo animal studies are necessary to evaluate a potential clinical usage for dental implant applications.