bluuebunny/crossref_metadata_2025 huggingface dataset:
Dynamic Changes in Flavor and Microbiota in Traditionally Fermented Bamboo Shoots (Chimonobambusa szechuanensis (Rendle) Keng f.)
Authors: Long Zhijian , Zhao Shilin , Xu Xiaofeng , Du Wanning , Chen Qiyang , Hu Shanglian
Published: August 2023

Dissecting flavor formation and microbial succession during traditional fermentation help to promote standardized and large-scale production in the sour shoot industry. The principal objective of the present research is to elucidate the interplay between the physicochemical attributes, flavor, and microbial compositions of sour bamboo shoots in the process of fermentation. The findings obtained from the principal component analysis (PCA) indicated notable fluctuations in both the physicochemical parameters and flavor components throughout the 28 day fermentation process. At least 13 volatile compounds (OAV > 1) have been detected as characteristic aroma compounds in sour bamboo shoots. Among these, 2,4-dimethyl Benzaldehyde exhibits the highest OAV (129.73~668.84) and is likely the primary contributor to the sour odor of the bamboo shoots. The analysis of the microbial community in sour bamboo shoots revealed that the most abundant phyla were Firmicutes and Proteobacteria, while the most prevalent genera were Enterococcus, Lactococcus, and Serratia. The results of the correlation analysis revealed that Firmicutes exhibited a positive correlation with various chemical compounds, including 3,6-nonylidene-1-ol, 2,4-dimethyl benzaldehyde, silanediol, dimethyl-, nonanal, and 2,2,4-trimethyl-1,3-pentylenediol diisobutyrate. Similarly, Lactococcus was found to be positively correlated with several chemical compounds, such as dimethyl-silanediol, 1-heptanol, 3,6-nonylidene-1-ol, nonanal, 2,2,4-trimethyl-1,3-pentanediol diisobutyrate, dibutyl phthalate, and TA. This study provides a theoretical basis for the standardization of traditional natural fermented sour bamboo production technology, which will help to further improve the flavor and quality of sour bamboo.
Lipid Process Markers of Durum Wheat Debranning Fractions
Authors: Marzocchi Silvia , Messia Maria Cristina , Marconi Emanuele , Caboni Maria Fiorenza , Pasini Federica
Published: August 2023

At present, whole grains are usually obtained by adding bran and middlings to refined flours, and this recombination leads to certain variations in the ratio of endosperm, bran and germ, resulting in flours with very different compositional characteristics and rheological properties. Therefore, this study focuses on the identification of specific lipid markers in different debranning fractions of Italian and Canadian durum wheat blends. The by-products obtained from five different debranning levels (3, 6, 9, 12 and 15%) had a high content of monounsaturated fatty acids and a higher concentration of tocopherols and sterols than the corresponding debranned grains. The Italian and Canadian durum wheat samples did not show significant differences in the content of these bioactive lipid compounds. In particular, palmitic acid, oleic acid, tocopherol isomers and total sterols could be useful biomarkers for evaluating the grain-to-tissue ratio in recombined flours.
Effects of Dietary Chlorogenic Acid Supplementation on Growth Performance, Meat Quality, and Muscle Flavor Substances in Finishing Pigs
Authors: Xie Kunhong , Sun Yaxin , Deng Lili , Yu Bing , Luo Yuheng , Huang Zhiqing , Mao Xiangbing , Yu Jie , Zheng Ping , Yan Hui , Li Yan , Li Hua , He Jun
Published: August 2023

With the prohibition of antibiotics in feed, certain phytocompounds have been widely studied as feed additives. Chlorogenic acid (CGA), a natural polyphenol found in plants, possesses anti-inflammatory, antioxidant, and metabolic regulatory features. The objective of this study was to investigate the effects of dietary chlorogenic acid supplementation on growth performance and carcass traits, as well as meat quality, nutrient value and flavor substances of Duroc × Landrace × Yorkshire (DLY) pigs. Forty healthy DLY pigs (initial body weight (BW): 26.69 ± 0.37) were allotted to four treatment groups and were fed with the control diet, which was supplemented with 25 mg kg−1, 50 mg kg−1, and 100 mg kg−1 CGA, respectively. The trial lasted 100 days. The results suggested that dietary CGA supplementation had no effect (p < 0.05) on the average daily gain (ADG) and feed conversion ratio (FC). Herein, it was found that 50 mg kg−1 CGA-containing diet not only increased the dressing percentage and perirenal fat, but also reduced the rate of muscular pH decline (p < 0.05). In the longissimus thoracis (LT) muscle, the myofiber-type-related genes such as the MyHC IIa and MyHC IIX mRNA levels were increased by 100 mg kg−1 CGA. The results also indicated that the 100 mg kg−1 CGA-containing diet increased the content of crude fat, glycogen, total amino acids, and flavor amino acids, but decreased the inosine and hypoxanthine concentration in LT (p < 0.05). Meanwhile, the lipogenic gene ACC1 mRNA level was elevated by 50 mg kg−1 CGA. Instead, 100 mg kg−1 CGA downregulated the expression level of NT5C2, an enzyme responsible for inosine-5′-monophosphate (IMP) degradation. Additionally, 100 mg kg−1 CGA decreased the malondialdehyde (MDA) content, but increased the glutathione peroxidase (GSH-Px) content as well as antioxidant gene (HO-1, NQO-1, NRF2) mRNA levels in LT muscle. These findings showed that dietary CGA could partly improve carcass traits and muscle flavor without negatively affecting growth performance, and the underlying mechanism may be due to the antioxidant properties induced by CGA.
Diagnostic Approach and Pathophysiological Mechanisms of Anemia in Chronic Liver Disease—An Overview
Authors: Marginean Cristina Maria , Pirscoveanu Denisa , Popescu Mihaela , Docea Anca Oana , Radu Antonia , Popescu Alin Iulian Silviu , Vasile Corina Maria , Mitrut Radu , Marginean Iulia Cristina , Iacob George Alexandru , Firu Dan Mihai , Mitrut Paul
Published: August 2023

Hematological abnormalities are frequently linked to chronic liver disease of any etiology. About 75% of patients with advanced chronic liver disease experience anemia. The causes of anemia are complex and multifactorial, particularly in cirrhotic patients. Acute and long-term blood loss from the upper gastrointestinal tract, malnutrition, an enlarged spleen brought on by portal hypertension, hemolysis, and coagulation issues are the main causes of anemia. Alcohol, a common cause of chronic liver disease, determines anemia through direct toxicity on the bone marrow, with the suppression of hematopoiesis, through vitamin B6, B12, and folate deficiency due to low intake and malabsorption. In patients with chronic hepatitis C virus infection, antiviral drugs such as pegylated interferon and ribavirin can also cause significant anemia. The use of interferon has been linked to bone marrow toxicity, and hemolytic anemia brought on by ribavirin is a well-known dose-dependent side effect. Within six months of the infection with hepatitis B, hepatitis C, and Epstein–Barr viruses, aplastic anemia associated with hepatitis is seen. This anemia is characterized by pancytopenia brought on by hypocellular bone marrow. Esophageal varices, portal hypertensive gastropathy, and gastric antral vascular ectasia can all cause acute and chronic blood loss. These conditions can progress to iron deficiency anemia, microcytic anemia, and hypochromic anemia. Another common hematologic abnormality in liver cirrhosis is macrocytosis, with multifactorial causes. Vitamin B12 and folate deficiency are frequent in liver cirrhosis, especially of alcoholic etiology, due to increased intestinal permeability, dysbiosis, and malnutrition. Many chronic liver diseases, like viral and autoimmune hepatitis, have a chronic inflammatory substrate. Proinflammatory cytokines, including tumor necrosis factor and interleukin 1, 6, and 10, are the main factors that diminish iron availability in progenitor erythrocytes and subsequent erythropoiesis, leading to the development of chronic inflammatory, normochromic, normocytic anemia.
Comprehensive Optimization of Western Blotting
Authors: Liu Dishiwen , Wu Haoliang , Cui Shengyu , Zhao Qingyan
Published: August 2023

Western blotting is one of the most extensively used techniques in the biomedical field. However, it is criticized by many researchers due to its considerable time consumption, multiple steps, and low method results. Therefore, we modified the steps of gel preparation, electrophoresis, electrotransfer, blocking, and gel cutting. First, we simplified the gel preparation step by premixing various reagents and varying the amounts of catalysts or radical generators, which shortened the entire process to 10 min. Second, we shortened the electrophoresis process to 35 min by modifying the formula of the electrophoresis running buffer. Then, we removed the hazard of methanol vapor by replacing methanol with ethanol in the electrotransfer buffer. Finally, the use of polyvinylpyrrolidone-40 shortened the blocking procedure to 10 min. Our modifications shortened the time, improved the experimental productivity, and minimized the experimental cost without hindering compatibility with most existing equipment. The entire experiment up to primary antibody incubation can be completed within 80 min.
Composite Hydrogels with Embedded Silver Nanoparticles and Ibuprofen as Wound Dressing
Authors: Popescu Irina , Constantin Marieta , Solcan Gheorghe , Ichim Daniela Luminita , Rata Delia Mihaela , Horodincu Loredana , Solcan Carmen
Published: August 2023

The wound healing process is often slowed down as a result of complications from bacterial infections and inflammatory reactions. Therefore, it is necessary to develop dressings with fast antibacterial and anti-inflammatory activity that shorten the wound healing period by promoting cell migration and proliferation. Chitosan (CS)-based hydrogels have been widely studied for their antibacterial and wound healing capabilities. Herein, we developed a composite hydrogel based on CS and PVA embedding silver nanoparticles (AgNPs) with antibacterial properties and ibuprofen (Ib) as an anti-inflammatory agent. The hydrogel prepared by double physical cross-linking, with oxalic acid and by freeze–thawing, loaded with 0.225 wt.% AgNPs and 0.264 wt.% Ib, displayed good mechanical properties (compressive modulus = 132 kPa), a high swelling degree and sustained drug delivery (in simulated skin conditions). Moreover, the hydrogel showed strong antibacterial activity against S. aureus and K. pneumoniae due to the embedded AgNPs. In vivo, this hydrogel accelerated the wound regeneration process through the enhanced expression of TNF alpha IP8, by activating downstream cascades and supporting the healing process of inflammation; Cox2, which enhances the migration and proliferation of cells involved in re-epithelization and angiogenesis; MHCII, which promotes immune cooperation between local cells, eliminating dead tissue and controlling infection; the intense expression of Col I as a major marker in the tissue granulation process; and αSMA, which marks the presence of myofibroblasts involved in wound closure and indicates ongoing re-epithelization. The results reveal the potential healing effect of CS/PVA/AgNPs/Ib hydrogels and suggest their potential use as wound dressings.
Evaluation of Poly(vinyl alcohol)–Xanthan Gum Hydrogels Loaded with Neomycin Sulfate as Systems for Drug Delivery
Authors: Serbezeanu Diana , Iftime Manuela Maria , Ailiesei Gabriela-Liliana , Ipate Alina-Mirela , Bargan Alexandra , Vlad-Bubulac Tǎchiţǎ , Rîmbu Cristina Mihaela
Published: August 2023

In recent years, multidrug-resistant bacteria have developed the ability to resist multiple antibiotics, limiting the available options for effective treatment. Raising awareness and providing education on the appropriate use of antibiotics, as well as improving infection control measures in healthcare facilities, are crucial steps to address the healthcare crisis. Further, innovative approaches must be adopted to develop novel drug delivery systems using polymeric matrices as carriers and support to efficiently combat such multidrug-resistant bacteria and thus promote wound healing. In this context, the current work describes the use of two biocompatible and non-toxic polymers, poly(vinyl alcohol) (PVA) and xanthan gum (XG), to achieve hydrogel networks through cross-linking by oxalic acid following the freezing/thawing procedure. PVA/XG-80/20 hydrogels were loaded with different quantities of neomycin sulfate to create promising low-class topical antibacterial formulations with enhanced antimicrobial effects. The inclusion of neomycin sulfate in the hydrogels is intended to impart them with powerful antimicrobial properties, thereby facilitating the development of exceptionally efficient topical antibacterial formulations. Thus, incorporating higher quantities of neomycin sulfate in the PVA/XG-80/20-2 and PVA/XG-80/20-3 formulations yielded promising cycling characteristics. These formulations exhibited outstanding removal efficiency, exceeding 80% even after five cycles, indicating remarkable and consistent adsorption performance with repeated use. Furthermore, both PVA/XG-80/20-2 and PVA/XG-80/20-3 formulations outperformed the drug-free sample, PVA/XG-80/20, demonstrating a significant enhancement in maximum compressive stress.
Polydopamine-Functionalized Bacterial Cellulose as Hydrogel Scaffolds for Skin Tissue Engineering
Authors: Narayanan Kannan Badri , Bhaskar Rakesh , Sudhakar Kuncham , Nam Dong Hyun , Han Sung Soo
Published: August 2023

Bacterial cellulose (BC) is a natural polysaccharide polymer hydrogel produced sustainably by the strain Gluconacetobacter hansenii under static conditions. Due to their biocompatibility, easy functionalization, and necessary physicochemical and mechanical properties, BC nanocomposites are attracting interest in therapeutic applications. In this study, we functionalized BC hydrogel with polydopamine (PDA) without toxic crosslinkers and used it in skin tissue engineering. The BC nanofibers in the hydrogel had a thickness of 77.8 ± 20.3 nm, and they could be used to produce hydrophilic, adhesive, and cytocompatible composite biomaterials for skin tissue engineering applications using PDA. Characterization techniques, namely Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and Raman spectroscopy, were performed to investigate the formation of polydopamine on the BC nanofibers. The XRD peaks for BC occur at 2θ = 14.65°, 16.69°, and 22.39°, which correspond to the planes of (100), (010), and (110) of cellulose type Iα. Raman spectroscopy confirmed the formation of PDA, as indicated by the presence of bands corresponding to the vibration of aromatic rings and aliphatic C–C and C–O stretching at 1336 and 1567 cm−1, respectively. FTIR confirmed the presence of peaks corresponding to PDA and BC in the BC/PDA hydrogel scaffolds at 3673, 3348, 2900, and 1052 cm−1, indicating the successful interaction of PDA with BC nanofibers, which was further corroborated by the SEM images. The tensile strength, swelling ratio, degradation, and surface wettability characteristics of the composite BC biomaterials were also investigated. The BC/PDA hydrogels with PDA-functionalized BC nanofibers demonstrated excellent tensile strength and water-wetting ability while maintaining the stability of the BC fibers. The enhanced cytocompatibility of the BC/PDA hydrogels was studied using the PrestoBlue assay. Culturing murine NIH/3T3 fibroblasts on BC/PDA hydrogels showed higher metabolic activity and enhanced proliferation. Additionally, it improved cell viability when using BC/PDA hydrogels. Thus, these BC/PDA composite biomaterials can be used as biocompatible natural alternatives to synthetic substitutes for skin tissue engineering and wound-dressing applications.
Identity Awoken in Second-Generation British Poles in the UK—Personal Journeys
Authors: Bifulco Antonia , Smojkis Maureen
Published: August 2023

We examine the identity of British Poles born in the UK, whose parents arrived as allied servicemen and their families, seeking asylum following WW2. The two authors are from this community, and here examine their British-Polish identity along with other second-generation Poles in the UK. These individuals grew up in distinct communities exposed to the Polish language and culture but with restricted contact with communist Poland. The themes of response to parents’ trauma experience, Polish identity in childhood and in midlife, Polish language, and visiting family in Poland were explored. Many described parents as secretive about the horrors of war but keen to retain and propagate their Polish identity. Some felt they were not fully Polish, but their identity increased with access to modern Poland as adults. The Polish language was important to identity but linked to feeling inadequacy when not fluent. Visiting family in Poland enhanced identity, was valued, and provided information on family history. European identity was adopted by some to cover both their British and Polish identity. Genealogy and family history are popular and linked to community, and British Poles have a distinct contribution and a voice in showing how identity can emerge out of family trauma.
Refining the Global Phylogeny of Mitochondrial N1a, X, and HV2 Haplogroups Based on Rare Mitogenomes from Croatian Isolates
Authors: Havaš Auguštin Dubravka , Šarac Jelena , Reidla Maere , Tamm Erika , Grahovac Blaženka , Kapović Miljenko , Novokmet Natalija , Rudan Pavao , Missoni Saša , Marjanović Damir , Korolija Marina
Published: August 2023

Mitochondrial DNA (mtDNA) has been used for decades as a predominant tool in population genetics and as a valuable addition to forensic genetic research, owing to its unique maternal inheritance pattern that enables the tracing of individuals along the maternal lineage across numerous generations. The dynamic interplay between evolutionary forces, primarily genetic drift, bottlenecks, and the founder effect, can exert significant influence on genetic profiles. Consequently, the Adriatic islands have accumulated a subset of lineages that exhibits remarkable absence or rarity within other European populations. This distinctive genetic composition underscores the islands’ potential as a significant resource in phylogenetic research, with implications reaching beyond regional boundaries to contribute to a global understanding. In the initial attempt to expand the mitochondrial forensic database of the Croatian population with haplotypes from small isolated communities, we sequenced mitogenomes of rare haplogroups from different Croatian island and mainland populations using next-generation sequencing (NGS). In the next step and based on the obtained results, we refined the global phylogeny of haplogroup N1a, HV2, and X by analyzing rare haplotypes, which are absent from the current phylogenetic tree. The trees were based on 16 novel and 52 previously published samples, revealing completely novel branches in the X and HV2 haplogroups and a new European cluster in the ancestral N1a variant, previously believed to be an exclusively African–Asian haplogroup. The research emphasizes the importance of investigating geographically isolated populations and their unique characteristics within a global context.
The Impact of Heavy Smoking on Male Infertility and Its Correlation with the Expression Levels of the PTPRN2 and PGAM5 Genes
Authors: Amor Houda , Alkhaled Yaser , Bibi Riffat , Hammadeh Mohamad Eid , Jankowski Peter Michael
Published: August 2023

Smoking has been linked to male infertility by affecting the sperm epigenome and genome. In this study, we aimed to determine possible changes in the transcript levels of PGAM5 (the phosphoglycerate mutase family member 5), PTPRN2 (protein tyrosine phosphatase, N2-type receptor), and TYRO3 (tyrosine protein kinase receptor) in heavy smokers compared to non-smokers, and to investigate their association with the fundamental sperm parameters. In total, 118 sperm samples (63 heavy-smokers (G1) and 55 non-smokers (G2)) were included in this study. A semen analysis was performed according to the WHO guidelines. After a total RNA extraction, RT-PCR was used to quantify the transcript levels of the studied genes. In G1, a significant decrease in the standard semen parameters in comparison to the non-smokers was shown (p < 0.05). Moreover, PGAM5 and PTPRN2 were differentially expressed (p ≤ 0.03 and p ≤ 0.01, respectively) and downregulated in the spermatozoa of G1 compared to G2. In contrast, no difference was observed for TYRO3 (p ≤ 0.3). In G1, the mRNA expression level of the studied genes was correlated negatively with motility, sperm count, normal form, vitality, and sperm membrane integrity (p < 0.05). Therefore, smoking may affect gene expression and male fertility by altering the DNA methylation patterns in the genes associated with fertility and sperm quality, including PGAM5, PTPRN2, and TYRO3.
LSS rs2254524 Increases the Risk of Hypertension in Children and Adolescents with Obesity
Authors: Umano Giuseppina Rosaria , Cirillo Grazia , Rondinelli Giulia , Sanchez Gianmaria , Marzuillo Pierluigi , Guarino Stefano , Di Sessa Anna , Papparella Alfonso , Miraglia del Giudice Emanuele
Published: August 2023

Childhood obesity and its related comorbidities have become major health issues over the last century. Among these comorbidities, cardiovascular diseases, especially hypertension, are the most significant. Recently, a polymorphism affecting the activity of lanosterol synthase has been associated with an increased risk of hypertension in adolescents. In this study, we aimed to investigate the effect of LSS rs2254524 polymorphism on blood pressure in children and adolescents with obesity. We enrolled 828 obese children aged 6–17 years. Subjects carrying the A allele showed higher rates of systolic and diastolic stage I hypertension and stage II hypertension. Carriers of the A allele showed a 2.4-fold (95% C.I. 1.5–4.7, p = 0.01) higher risk for stage II hypertension and a 1.9-fold higher risk for stage I hypertension (95% C.I. 1.4–2.6, p < 0.0001). The risk was independent of confounding factors. In conclusion, LSS rs2254524 worsens the cardiovascular health of children and adolescents with obesity, increasing their blood pressure.
Tunnelling with Full-Face Shielded Machines: A 3D Numerical Analysis of an Earth Pressure Balance (EPB) Excavation Sequence Using the Finite Element Method (FEM)
Authors: Tyrer Jonathan , Paraskevopoulou Chrysothemis , Shah Ravi , Miller Richard , Kavvadas Michael
Published: August 2023

Urban tunnelling can be highly challenging, especially in areas where limited ground settlements and environmental disturbance is required. Mechanised tunnelling is usually preferred in such ground environments, specifically Slurry or EPBM (Earth Pressure Balance Machine), depending on the ground properties. Being able to predict the anticipated tunnel behaviour at the preliminary stages of the project can be very beneficial in optimising not only the design, but also control the construction activities and completion times. In practice, the short-term excavation response and support performance focus primarily on design, since most site characterisation inputs are focused on material properties gained from short-term testing. Although the analysis of tunnelling is a three-dimensional (3D) problem, conventional approaches and design methods employed during the design and construction of underground openings are often based on the ground’s static response in two dimensions (2D). In this paper, an initial 2D model is generated in PLAXIS2D and RS2 (Rocscience) to test advanced constitutive models and compare transverse settlement profiles; subsequently, a complete 3D FEM numerical model in RS3 (Rocscience) was used to simulate an Earth Pressure Balance (EPB) excavation sequence. The 3D numerical model simulates the relevant EPB components such as face pressure, TBM shield, backfilling of the tail void (time-dependent hardening of the grout) and gradual segmental lining erections in the longitudinal direction. The presented numerical approach can be used by tunnel designers and engineers to predict the soil response in EPBM tunnelling.
Atherogenic Activation of Human Vascular Smooth Muscle Cells by Monosodium Urate Crystals
Authors: Liu-Bryan Ru , Guo Tracy , Lee Jennifer , Terkeltaub Robert
Published: August 2023

Gout is strongly associated with atherosclerosis and other cardiovascular comorbidities. Furthermore, sites of extra-articular monosodium urate (MSU) crystal deposits in gout can include heart valves and atherosclerotic artery plaques, but with unclear effects therein. Hence, we seminally explored cultured vascular smooth muscle cell (VSMC) responsiveness to MSU crystals. To limit confounding effects, we cultured human aortic VSMCs under serum-free conditions to assess MSU crystal effects on VSMC differentiation and function, differentially expressed genes (DEGs) via RNA sequencing, and selected atherogenic changes in cytokines and the lipidome. MSU crystals induced p38 phosphorylation, IL-6, and VSMC vacuolization with dysregulated autophagy. MSU-crystal-induced DEGs included decreased late-stage autophagosome maturation mediator GABARAPL1, decreased physiologic VSMC differentiation regulators (LMOD1 and SYNPO2), increased ATF4, CHOP, and the intrinsic apoptosis signaling pathway in response to ER stress, and neointimal atherogenic nuclear receptors (NR4A1 and NR4A3). MSU crystals alone increased the levels of cholesterol biosynthetic intermediates 14-demethyl-lanosterol (14-DML), desmosterol, and zymosterol. Adding MSU crystals increased oxidized LDL’s capacity to increase intracellular 27-OH cholesterol, and MSU crystals and oxidized LDL synergistically induced a marked release of arachidonate. In conclusion, MSU crystals deposited in arterial media and neointima have the potential to dysregulate VSMC differentiation and proteostasis, and to induce further atherogenic effects, which include enhanced VSMC loading of oxidized cholesterol intermediates and release of IL-6 and arachidonic acid (AA).
On Displacement and the Humanities—An Introduction
Authors: Isayev Elena , Jewell Evan , Gizaw Gerawork Teferra , Schenck Marcia C.
Published: August 2023

When we conceived of the volume, Displacement and the Humanities: Manifestos from the Ancient to the Present, six years ago, important and urgent studies on the subject of migration had increased substantially over the past decade in response to what has been termed the ‘migration crisis’ [...]
Power and Subjectification at the Edge of Social Media Interfaces in the Aftermath of the Jallikattu Protest
Authors: Prince Deepak
Published: August 2023

In January 2017, millions of people occupied various public places across the southern Indian state of Tamil Nadu, protesting the Supreme Court’s ban on Jallikattu, a bull-wrangling contest considered central to Tamil identity. Social media was thought to have triggered this ‘leaderless’ protest. Seven days in, a police crackdown splintered the protest’s seemingly unified front. Academic commentators have argued that social media present radical possibilities, ‘short-circuiting’ older forms of broadcast media, which had already been colonized by the state. Taking as discursive sites two videos, one of them posted by a popular Facebook group and another by a YouTube channel centred around Dalit issues, I argue that an a priori claim of new media having a lesser or greater potential to resist colonization is largely untenable. The possibility of such resistance is contingent on the micropolitics of contestation within concrete, localized sites. I analyse narratives of loss and rage on two different social media spaces, elicited from a fishing community near one of the protest sites, after their homes were attacked and their local market had been burnt down by the police. By focusing on tactics of interviewing, I demonstrate that, in the span of a week, the same technological platform credited with sparking the protests that brought the Tamils together as one, now constitutes the limits of the formation of radical subjectivity, as Tamil society finds itself fractured once again.
Protocol for Community-Based Exercise Training after Discharge from Hospital-Based Stroke Rehabilitation: A Multicenter, Randomized, Parallel-Group, Double-Blind Controlled Pilot and Feasibility Trial
Authors: Kang Dongheon , Park Jiyoung , Eun Seon-Deok
Published: August 2023

Exercise training participation of patients with stroke in the community after discharge from the hospital has many benefits for physical, social, and psychological rehabilitation and improves their quality of life. However, in the Republic of Korea, studies on stroke survivors who can participate in an exercise training program have not been conducted. This trial aims to investigate the effectiveness of exercise training programs after patients with stroke are discharged from the hospital with a doctor’s note and referred to a community exercise center, as there is a lack of studies on this population. This multicenter, randomized, parallel-group, double-blind controlled pilot and feasibility trial will randomly assign 120 patients with stroke to either 8 weeks of a community-based exercise training program (experimental group) or activities of daily living (control group). The primary outcomes will be muscle strength, cardiorespiratory fitness, body composition, physical performance, and gait. The secondary outcomes will be quality of life and activities of daily living. This study’s results may add new insights into the effectiveness of community-based exercise training programs after patients with stroke are discharged from the hospital with a doctor’s note and referred to a community exercise center. The success of the new exercise training approach could offer valuable information for developing more inclusive protocols for patients with stroke in the future if it proves to be efficacious.
Studying Dynamical Characteristics of Oxygen Saturation Variability Signals Using Haar Wavelet
Authors: Alassafi Madini O. , Khan Ishtiaq Rasool , AlGhamdi Rayed , Aziz Wajid , Alshdadi Abdulrahman A. , Dessouky Mohamed M. , Bahaddad Adel , Altalbe Ali , Albishry Nabeel
Published: August 2023

An aim of the analysis of biomedical signals such as heart rate variability signals, brain signals, oxygen saturation variability (OSV) signals, etc., is for the design and development of tools to extract information about the underlying complexity of physiological systems, to detect physiological states, monitor health conditions over time, or predict pathological conditions. Entropy-based complexity measures are commonly used to quantify the complexity of biomedical signals; however novel complexity measures need to be explored in the context of biomedical signal classification. In this work, we present a novel technique that used Haar wavelets to analyze the complexity of OSV signals of subjects during COVID-19 infection and after recovery. The data used to evaluate the performance of the proposed algorithms comprised recordings of OSV signals from 44 COVID-19 patients during illness and after recovery. The performance of the proposed technique was compared with four, scale-based entropy measures: multiscale entropy (MSE); multiscale permutation entropy (MPE); multiscale fuzzy entropy (MFE); multiscale amplitude-aware permutation entropy (MAMPE). Preliminary results of the pilot study revealed that the proposed algorithm outperformed MSE, MPE, MFE, and MMAPE in terms of better accuracy and time efficiency for separating during and after recovery the OSV signals of COVID-19 subjects. Further studies are needed to evaluate the potential of the proposed algorithm for large datasets and in the context of other biomedical signal classifications.
The Impact of Sexual Violence on Quality of Life and Mental Wellbeing in Transgender and Gender-Diverse Adolescents and Young Adults: A Mixed-Methods Approach
Authors: Burgwal Aisa , Van Wiele Jara , Motmans Joz
Published: August 2023

Transgender (trans) and gender-diverse (GD) adolescents and young adults have remained largely invisible in health research. Previous research shows worse outcomes in health indicators for trans and GD people, compared to cisgender controls. Research on the impact of sexual violence focuses on mainly cisgender female adult victims. This study assessed the impact of sexual violence on the quality of life (QoL) and mental wellbeing (GHQ-12) among trans and GD adolescents and young adults, while taking into account the possible role of gender nonconformity in sexual violence and mental wellbeing. An online, anonymous survey and interviews/focus groups were conducted between October 2021 and May 2022 in Belgium. Multiple analyses of covariance (ANCOVAs) were used to assess the associations between sexual violence, mental wellbeing, and gender nonconformity, while controlling for different background variables (gender identity, sexual orientation, age, economic vulnerability, etc.). The interviews and focus groups were used to validate associations between variables that were hypothesized as important. The quantitative sample consisted of 110 respondents between 15 and 25 years old, with 30 trans respondents (27.3%) and 80 GD respondents (72.7%). A total of 73.6% reported experiences with sexual violence over the past two years (n = 81). The mean QoL score was 5.3/10, and the mean GHQ-12 score was 6.6/12. Sexual violence was not significantly associated with QoL (p = 0.157) and only marginally significantly associated with GHQ-12 (p = 0.05). Changing one’s physical appearance to conform to gender norms, out of fear of getting attacked, discriminated against, or harassed was significantly associated with QoL (p = 0.009) and GHQ-12 (p = 0.041). The association between sexual violence and changing one’s physical appearance to conform to gender norms was analyzed, to assess a possible mediation effect of sexual violence on mental wellbeing. No significant association was found (p = 0.261). However, the interviews suggest that sexual violence is associated with changing one’s physical appearance, but this association is not limited to only trans and GD victims of sexual violence. Non-victims also adjust their appearance, out of fear of future sexual victimization. Together with the high proportion of sexual violence, as well as the lower average QoL and higher average GHQ-12 scores among trans and GD adolescents and young adults, compared to general population statistics, this highlights the need for policy makers to create more inclusive environments.
Socio-Demographic Factors and Public Knowledge of Antibiotic Resistance
Authors: Bhatt Vedika , Ponnampalavanar Sasheela Sri La Sri , Chong Chun Wie , Tang Li Yoong , Krishnasamy Karthikayini , Goh Sheron Sir Loon , Teh Cindy Shuan Ju
Published: August 2023

(1) Background: Antibiotic resistance is a serious health issue, and raising public awareness of it is crucial to combating it. This study aimed to assess the socio-demographic factors associated with knowledge of antibiotics and antibiotic resistance in Malaysia. (2) Methods: A cross-sectional study was carried out between April 2022 and March 2023. Malaysian adults aged ≥18 years old and able to understand English or Malay were recruited. During data collection, the WHO questionnaire “Antibiotic Resistance, Multi-Country Public Awareness Survey” was used. Data were collected across 14 states in Malaysia. (3) Results: A total of 517 participants completed the questionnaire. Most participants were females (67.9%), aged 30–49 (46%), and from central Malaysia (69.8%). Most participants (98.5%) reported taking antibiotics. A misconception presented was that sore throats, fevers, colds, and flu can be treated with antibiotics. A total of 58.8% of participants had high knowledge of antibiotic usage (scores 12–15), while 64% had high knowledge of antibiotic resistance (scores 9–14). Findings indicate that increasing age, income, and education were associated with higher knowledge. (4) Conclusions: This study highlights the knowledge deficiency of antibiotic resistance among Malaysians. Educational programs should engage a younger and lower socio-economic population to increase awareness.
Participants Characteristics of a Park-Based Physical Activity Intervention in an Urban Context—A Cross-Sectional Study in Bologna, Italy
Authors: Bianconi Alessandro , Masini Alice , Sanmarchi Francesco , Toselli Stefania , Grigoletto Alessia , Marini Sofia , Barone Giuseppe , Pinelli Erika , Zinno Raffaele , Mauro Mario , Pilone Pietro Loro , Arduini Sonia , Vitiello Mauro , Vicentini Bruno , Boldrini Giorgia , Musti Muriel Assunta , Pandolfi Paolo , Liberti Maurizio , Astorino Gerardo , Latessa Pasqualino Maietta , Bragonzoni Laura , Dallolio Laura
Published: August 2023

Physical inactivity, a leading risk factor for chronic diseases and premature death, is prevalent worldwide. This study focuses on participant profiles and factors influencing continued participation and physical activity (PA) levels in the “Moving Parks” PA intervention in Bologna, Italy. This intervention offers free group activities in city parks during the summer. A questionnaire was administered to participants in 2022, gathering data on socio-demographic information, chronic diseases, participation in previous iterations, and PA levels. Out of 596 respondents, 85% were women, and the average age was 50. About 56% held a university degree, and 73% exercised regularly in the winter. Continuous participation was linked to older age, higher education, and chronic conditions. Prior participation predicted higher winter PA levels. Notably, the majority of participants were educated, physically active women. While the project continually engages individuals with higher education and chronic conditions, it needs further tailoring to reach less represented populations.
Development and Integration of Patient-Reported Measures into E-Health System: Pilot Feasibility Study
Authors: Vaitkevičienė Goda Elizabeta , Ažukaitis Karolis , Jankauskienė Augustina , Petrėnė Justė , Puronaitė Roma , Trinkūnas Justas , Jankauskienė Danguolė
Published: August 2023

Patient-centered care is recognized as a key element in recent healthcare management strategies. However, the integrated collection of patient feedback capturing the entire journey of patients with complex medical conditions remains understudied. Herein, we aimed to describe the development of an instrument prototype for the collection of PROMs and PREMs that would encompass a whole patient journey at a single time point. We further describe the process of its integration into a hospital’s information system (HIS) and the results of a pilot feasibility study in adult patients with kidney and hematological diseases. We developed an instrument consisting of original PREM and generic EQ-5D-5L questionnaires. E-questionnaires were handled with REDCap software (version 12.5.14) and integrated into the HIS. Patients refusing to use e-questionnaires (48%) were offered paper administration and were older (64 vs. 50 years). The overall response rate for e-questionnaires was 57.1% with a median completion time of 2.0 and 3.7 min for PROM and PREM, respectively. Psychological and social services and primary care setting (diagnosis establishment and involvement in continuous care) were identified as most problematic. The majority of PREM dimensions encompassing different levels of care significantly correlated with PROM responses. Our data indicate the feasibility and potential relevance of the proposed approach, although wider-scale studies in diverse settings are needed.
Narrowing the Definition of Social Inclusion in Sport for People with Disabilities through a Scoping Review
Authors: Pečnikar Oblak Viktorija , Campos Maria João , Lemos Susana , Rocha Micaela , Ljubotina Predrag , Poteko Kaja , Kárpáti Orsolya , Farkas Judit , Perényi Szilvia , Kustura Urška , Massart Alain , Doupona Mojca
Published: August 2023

The concept and practice of social inclusion in sport are still undefined, causing confusion both in the field of sport policy and practice. According to the United Nations (UN), a conceptual and analytical work on what constitutes inclusion is needed. Therefore, this study aims to define social inclusion in sport for people with disabilities by reviewing the existing literature. Using a scoping review framework, articles related to a possible definition of social inclusion in sport or to the elements of this definition were reviewed. For the eighteen (18) articles selected, the focus was on 152 statements, which were grouped into 6 main categories, namely: policy (29), fundamental conditions (28), key elements (30), soft skills (20), field gaps (31), and best practices (14). Ten keywords were extracted from each of the six categories using the free online program cortical.io. All 60 keywords were then compared with each other. After deleting the duplicates, 24 keywords remained, which were classified into five major categories: (1) key people, (2) key environments, (3) key ways to use, (4) key benefits, and (5) key barriers, in order to create a descriptive definition of social inclusion in sport for people with disabilities that can contribute to the goals of the UN 2030 Agenda. In addition to the definition, relevant issues were also raised for in-depth discussion and further research.
DS-CNN: Deep Convolutional Neural Networks for Facial Emotion Detection in Children with Down Syndrome during Dolphin-Assisted Therapy
Authors: Moreno Escobar Jesús Jaime , Morales Matamoros Oswaldo , Aguilar del Villar Erika Yolanda , Quintana Espinosa Hugo , Chanona Hernández Liliana
Published: August 2023

In Mexico, according to data from the General Directorate of Health Information (2018), there is an annual incidence of 689 newborns with Trisomy 21, well-known as Down Syndrome. Worldwide, this incidence is estimated between 1 in every 1000 newborns, approximately. That is why this work focuses on the detection and analysis of facial emotions in children with Down Syndrome in order to predict their emotions throughout a dolphin-assisted therapy. In this work, two databases are used: Exploratory Data Analysis, with a total of 20,214 images, and the Down’s Syndrome Dataset database, with 1445 images for training, validation, and testing of the neural network models. The construction of two architectures based on a Deep Convolutional Neural Network manages an efficiency of 79%, when these architectures are tested with a large reference image database. Then, the architecture that achieves better results is trained, validated, and tested in a small-image database with the facial emotions of children with Down Syndrome, obtaining an efficiency of 72%. However, this increases by 9% when the brain activity of the child is included in the training, resulting in an average precision of 81%. Using electroencephalogram (EEG) signals in a Convolutional Neural Network (CNN) along with the Down’s Syndrome Dataset (DSDS) has promising advantages in the field of brain–computer interfaces. EEG provides direct access to the electrical activity of the brain, allowing for real-time monitoring and analysis of cognitive states. Integrating EEG signals into a CNN architecture can enhance learning and decision-making capabilities. It is important to note that this work has the primary objective of addressing a doubly vulnerable population, as these children also have a disability.
Virtual Reality Simulation for Advanced Infection Control Education in Neonatal Intensive Care Units: Focusing on the Prevention of Central Line-Associated Bloodstream Infections and Ventilator-Associated Infections
Authors: Ryu Jimin , Yu Mi
Published: August 2023

This study examined the effects of a virtual reality simulation for advanced infection control education in neonatal intensive care units (VR_AICENICU) on nurses’ infection control knowledge and performance confidence. We examined nurses’ presence, empathy, and program satisfaction using a non-equivalent control group pre-post design. Nurses were divided into an experimental group (n = 20) experiencing VR simulation and routine NICU practice and a control group (n = 20) with routine NICU practice. The VR_AICENICU program comprised three scenarios: high-risk medication with lipid solution, dressing and management for peripheral inserted central line, and aspiration prevention and skincare management during ventilator use for premature infants. Data were collected between February and July 2022. The experimental group showed significantly greater improvements in infection control knowledge and performance confidence compared to the pre-test. The average scores of presence, empathy, and program satisfaction of the experimental group were 4.39 ± 0.36, 4.33 ± 0.75, and 4.90 ± 0.31, respectively. The VR_AICENICU program has implications for the education needs of nurses working in NICUs and enhances their knowledge and performance of infection control. Future studies should apply the VR_AICENICU program to different severity grades of NICU patients and develop additional VR programs.
Identification of the Groundwater Quality and Potential Noncarcinogenic Health Risk Assessment of Nitrate in the Groundwater of El Milia Plain, Kebir Rhumel Basin, Algeria
Authors: Brella Djouhaina , Belkhiri Lazhar , Tiri Ammar , Salhi Hichem , Lakouas Fatma Elhadj , Nouibet Razki , Amrane Adeltif , Merdoud Ryma , Mouni Lotfi
Published: August 2023

In this study, we analyzed the quality and the potential noncarcinogenic health risk of nitrate in groundwater in the El Milia plain, Kebir Rhumel Basin, Algeria. Moran’s I and the ordinary kriging (OK) interpolation technique were used to examine the spatial distribution pattern of the hydrochemical parameters in the groundwater. It was found that the hydrochemical parameters Ca, Cl, and HCO3 showed strong spatial autocorrelation in the El Milia plain, indicating a spatial dependence and clustering of these parameters in the groundwater. The groundwater quality was evaluated using the entropy water quality index (EWQI). The results showed that approximately 86% of the total groundwater samples in the study area fall within the moderate groundwater quality category. The spatial map of the EWQI values indicated an increasing trend from the south-west to the northeast, following the direction of groundwater flow. The highest EWQI values were observed near El Milia city in the center of the plain. This spatial pattern suggests variations in groundwater quality across the study area, with potentially higher risks near the city center. The potential noncarcinogenic health risks associated with nitrate contamination in groundwater for adults and children through the drinking water pathway were assessed using the hazard quotient (HQ). The results revealed that approximately 5.7% of the total groundwater samples exceeded the HQ limit for adults, indicating potential health risks. Moreover, a higher percentage, 14.28%, of the total groundwater samples exceeded the HQ limit for children, highlighting their increased vulnerability to noncarcinogenic health hazards associated with nitrate contamination in the study area. Taking timely action and ensuring strict compliance with regulations in groundwater management are crucial for protecting public health, preserving the environment, addressing water scarcity, and achieving sustainable development goals.