bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy of ibuprofen and indomethacin as prophylaxis of heterotopic ossification: a comparative study
Authors: Schneider Jens , Maffulli Nicola , Eschweiler Jörg , Bell Andreas , Hildebrand Frank , Migliorini Filippo
Published: November 2023

The prophylactic action of non-steroidal anti-inflammatory drugs (NSAIDs) in heterotopic ossification (HO) was first described following analgesic therapy with indomethacin. Following that evidence, several compounds have been successfully used for prophylaxes of HO. Ibuprofen has been also proposed for the prevention of HO following THA. The present study compared the administration of ibuprofen for three weeks versus indomethacin as prophylaxis for HO following primary THA. In all THA procedures, pre- and post-operative protocols were conducted in a highly standardized fashion. The type of HO prophylaxis (indomethacin 100 mg/daily or ibuprofen 100 mg/daily) was chosen according to a chronological criterion: from 2017 to 2019 indomethacin was used, whereas from 2019 to 2022 ibuprofen was administered. In case of allergy or intolerance to NSAIDs, no prophylaxis was performed, and patients were included as a control group. All patients who underwent an anteroposterior radiography of the pelvis at a minimum of 12 months following THA were considered for inclusion. On admission, the age and sex of the patients were recorded. Moreover, the causes of osteoarthritis and the date of surgery were recorded. The grade of HO was assigned by a blinded assessor who was not involved in the clinical management of the patients. The modified Brooker Staging System was used to rate the efficacy of the interventions. Data from 1248 patients were collected. 62% (767 of 1248 patients) were women. The mean age was 67.0 ± 2.9 years. The mean follow-up was 21.1 ± 10.8 months. In the ibuprofen group, 73% of patients evidenced Brooker 0, 17% Brooker I, and 10% Brooker II. In the indomethacin group, 77% of patients evidenced Brooker 0, 16% Brooker I, 6% Brooker II. No patient in the ibuprofen and indomethacin group developed Brooker III or IV. In the control group, 64% of patients evidenced Brooker 0, 21% Brooker I, 3% Brooker II, and 12% Brooker III. No patient in the control group developed Brooker IV HO. Concluding, three weeks of administration of ibuprofen demonstrated similar efficacy to indomethacin in preventing HO following primary THA. The prophylaxis with ibuprofen or indomethacin was more effective in preventing HO compared to a control group who did not receive any pharmacological prophylaxis.
Survival following complete resection of neuroblastoma in novel orthotopic rat xenograft model
Authors: Sever ReidAnn E. , Rosenblum Lauren Taylor , Reyes-Múgica Miguel , Edwards W. Barry , Malek Marcus M. , Kohanbash Gary
Published: November 2023

Neuroblastoma accounts for 15% of pediatric cancer deaths, despite multimodal therapy including surgical resection. Current neuroblastoma rodent models are insufficient for studying the impact of surgery and combination treatments, largely due to the small size of mouse models. Human neuroblastoma SK-N-BE(2) cells were injected into the left adrenal gland of 5–6-week-old RNU homozygous nude rats. Rats were either monitored by MRI until humane endpoint was reached or after 5 weeks underwent operative tumor resection, followed by monitoring for recurrence and survival. Following neuroblastoma cell implantation, the majority of tumors grew to greater than 5000 mm3 within 5.5–6.5 weeks, meeting the humane endpoint. Surgical resection was successfully done in 8 out of 9 rats, extending survival following tumor implantation from a median of 42 days to 78 days (p < 0.005). Pathology was consistent with human neuroblastoma, showing small round blue cell tumors with Homer-Wright rosettes, high mitoses and karyorrhectic index, and strong PHOX2B staining. Thus, we have established a novel orthotopic xenograft rat model of neuroblastoma and demonstrated increased survival of rats after surgical tumor resection. This model can be used for the development of surgical techniques, such as the use of intraoperative molecular imaging or assessment of combination therapies that include surgery.
Sediment core analysis using artificial intelligence
Authors: Di Martino Andrea , Carlini Gianluca , Castellani Gastone , Remondini Daniel , Amorosi Alessandro
Published: November 2023

Subsurface stratigraphic modeling is crucial for a variety of environmental, societal, and economic challenges. However, the need for specific sedimentological skills in sediment core analysis may constitute a limitation. Methods based on Machine Learning and Deep Learning can play a central role in automatizing this time-consuming procedure. In this work, using a robust dataset of high-resolution digital images from continuous sediment cores of Holocene age that reflect a wide spectrum of continental to shallow-marine depositional environments, we outline a novel deep-learning-based approach to perform automatic semantic segmentation directly on core images, leveraging the power of convolutional neural networks. To optimize the interpretation process and maximize scientific value, we use six sedimentary facies associations as target classes in lieu of ineffective classification methods based uniquely on lithology. We propose an automated model that can rapidly characterize sediment cores, allowing immediate guidance for stratigraphic correlation and subsurface reconstructions.
Investigation of antimicrobial susceptibility and genetic diversity among Staphylococcus pseudintermedius isolated from dogs in Rio de Janeiro
Authors: Teixeira Izabel Mello , de Moraes Assumpção Yasmim , Paletta Ana Clara Cabral , Aguiar Louise , Guimarães Luciana , da Silva Isabella Thomaz , Côrtes Marina F. , Botelho Ana Maria Nunes , Jaeger Lauren Hubert , Ferreira Renata Fernandes , de Oliveira Ferreira Eliane , Penna Bruno
Published: November 2023

Staphylococcus pseudintermedius is an opportunistic pathogen causing a variety of infections that are difficult to treat, especially because of the development of antimicrobial resistance. It has a clonal distribution around the world. To have a better understanding of the MRSP population, we search the presence of MRSP in colonized or infected dogs. Samples from 99 dogs with infections and 35 from asymptomatic dogs were collected. Isolates were identified by mass spectrometry and Multiplex-PCR. The mecA gene was confirmed by conventional PCR. MRSP strains were analyzed by whole-genome sequencing. 75 S. pseudintermedius were identified, most from infection cases. The species were isolated from 70 out of the 135 dogs. Penicillin and Trimethoprim/Sulfamethoxazole presented higher resistance rates. Forty-seven strains were classified as multi-drug resistant (MDR), and were more isolated from dogs with infection (P < 0.05). Eighteen samples were classified as MRSP, representing 24.0% of the population. Six of 16 MRSP sequenced samples belonged to the world spread clone ST71; others belonged to unknown clones. Most samples carried the SCCmec type IIIA. Twenty-one different genetic resistance determinants were found among MRPS strains. MRSP is circulating among infected and colonized dogs in Rio de Janeiro, Brazil.
Mapping brucellosis risk in Kenya and its implications for control strategies in sub-Saharan Africa
Authors: Akoko James M. , Mwatondo Athman , Muturi Mathew , Wambua Lillian , Abkallo Hussein M. , Nyamota Richard , Bosire Caroline , Oloo Stephen , Limbaso Konongoi S. , Gakuya Francis , Nthiwa Daniel , Bartlow Andrew , Middlebrook Earl , Fair Jeanne , Ogutu Joseph O. , Gachohi John , Njenga Kariuki , Bett Bernard
Published: November 2023

In Sub-Saharan Africa (SSA), effective brucellosis control is limited, in part, by the lack of long-term commitments by governments to control the disease and the absence of reliable national human and livestock population-based data to inform policies. Therefore, we conducted a study to establish the national prevalence and develop a risk map for Brucella spp. in cattle to contribute to plans to eliminate the disease in Kenya by the year 2040. We randomly generated 268 geolocations and distributed them across Kenya, proportionate to the area of each of the five agroecological zones and the associated cattle population. Cattle herds closest to each selected geolocation were identified for sampling. Up to 25 cattle were sampled per geolocation and a semi-structured questionnaire was administered to their owners. We tested 6,593 cattle samples for Brucella immunoglobulin G (IgG) antibodies using an Enzyme-linked immunosorbent assay (ELISA). We assessed potential risk factors and performed spatial analyses and prevalence mapping using approximate Bayesian inference implemented via the integrated nested Laplace approximation (INLA) method. The national Brucella spp. prevalence was 6.8% (95% CI: 6.2–7.4%). Exposure levels varied significantly between agro-ecological zones, with a high of 8.5% in the very arid zone with the lowest agricultural potential relative to a low of 0.0% in the agro-alpine zone with the highest agricultural potential. Additionally, seroprevalence increased with herd size, and the odds of seropositivity were significantly higher for females and adult animals than for males or calves. Similarly, animals with a history of abortion, or with multiple reproductive syndromes had higher seropositivity than those without. At the herd level, the risk of Brucella spp. transmission was higher in larger herds, and herds with a history of reproductive problems such as abortion, giving birth to weak calves, or having swollen testes. Geographic localities with high Brucella seroprevalence occurred in northern, eastern, and southern regions of Kenya all primarily characterized by semi-arid or arid agro-ecological zones dominated by livestock pastoralism interspersed with vast areas with mixed livestock-wildlife systems. The large spatial extent of our survey provides compelling evidence for the widespread geographical distribution of brucellosis risk across Kenya in a manner easily understandable for policymakers. Our findings can provide a basis for risk-stratified pilot studies aiming to investigate the cost-effectiveness and efficacy of singular and combined preventive intervention strategies that seek to inform Kenya’s Brucellosis Control Policy.
Minimally invasive management of vital teeth requiring root canal therapy
Authors: Karatas E. , Hadis M. , Palin W. M. , Milward M. R. , Kuehne S. A. , Camilleri J.
Published: November 2023

The present study aimed to investigate the possible use of a non-instrumentation technique including blue light irradiation for root canal cleaning. Extracted human single rooted teeth were selected. Nine different groups included distilled water, NaOCl, intra-canal heated NaOCl, and NaOCl + EDTA irrigation after either instrumentation or non-instrumentation, and a laser application group following non-instrumentation technique. The chemical assessment of the root canal dentine was evaluated using energy dispersive spectroscopy (EDS) and Fourier transform infrared (FT-IR) spectroscopy. Surface microstructural analyses were performed by using scanning electron microscopy (SEM). The antimicrobial efficacy of different preparation techniques was evaluated using microbial tests. Light application didn’t change the calcium/phosphorus, carbonate/phosphate and amide I/phosphate ratios of the root canal dentin. The root canal dentin preserved its original chemistry and microstructure after light application. The instrumentation decreased the carbonate/phosphate and amide I/phosphate ratios of the root canal dentin regardless of the irrigation solution or technique (p < 0.05). The application of light could not provide antibacterial efficacy to match the NaOCl irrigation. The NaOCl irrigation both in the non-instrumentation and instrumentation groups significantly reduced the number of bacteria (p < 0.05). The use of minimally invasive root canal preparation techniques where the root canal is not instrumented and is disinfected by light followed by obturation with a hydraulic cement sealer reduced the microbial load and preserved the dentin thus may be an attractive treatment option for management of vital teeth needing root canal therapy.
CaV3.1 channels facilitate calcium wave generation and myogenic tone development in mouse mesenteric arteries
Authors: El-Lakany Mohammed A. , Haghbin Nadia , Arora Naman , Hashad Ahmed M. , Mironova Galina Yu. , Sancho Maria , Gros Robert , Welsh Donald G.
Published: November 2023

The arterial myogenic response to intraluminal pressure elicits constriction to maintain tissue perfusion. Smooth muscle [Ca2+] is a key determinant of constriction, tied to L-type (CaV1.2) Ca2+ channels. While important, other Ca2+ channels, particularly T-type could contribute to pressure regulation within defined voltage ranges. This study examined the role of one T-type Ca2+ channel (CaV3.1) using C57BL/6 wild type and CaV3.1−/− mice. Patch-clamp electrophysiology, pressure myography, blood pressure and Ca2+ imaging defined the CaV3.1−/− phenotype relative to C57BL/6. CaV3.1−/− mice had absent CaV3.1 expression and whole-cell current, coinciding with lower blood pressure and reduced mesenteric artery myogenic tone, particularly at lower pressures (20–60 mmHg) where membrane potential is hyperpolarized. This reduction coincided with diminished Ca2+ wave generation, asynchronous events of Ca2+ release from the sarcoplasmic reticulum, insensitive to L-type Ca2+ channel blockade (Nifedipine, 0.3 µM). Proximity ligation assay (PLA) confirmed IP3R1/CaV3.1 close physical association. IP3R blockade (2-APB, 50 µM or xestospongin C, 3 µM) in nifedipine-treated C57BL/6 arteries rendered a CaV3.1−/− contractile phenotype. Findings indicate that Ca2+ influx through CaV3.1 contributes to myogenic tone at hyperpolarized voltages through Ca2+-induced Ca2+ release tied to the sarcoplasmic reticulum. This study helps establish CaV3.1 as a potential therapeutic target to control blood pressure.
IGSF6 is a novel biomarker to evaluate immune infiltration in mismatch repair-proficient colorectal cancer
Authors: Rong Yu-ming , Xu Yu-cheng , Chen Xiao-chuan , Zhong Min-er , Tan Ying-xin , Liang Yu-fan , Weng Jing-rong , Liu Jun , Wang Xin-you , Luo Dan-dong , Bie Yi-ran , Chen Xi , Cai Jia-wei , Yu Zhao-liang , Zou Yi-feng
Published: November 2023

Immunotherapy has dramatically changed the landscape of treatment for colorectal cancer (CRC), but currently lack of effective predictive biomarker, especially for tumors with mismatch repair (MMR) proficiency. The response of immunotherapy is associated with the cell–cell interactions in tumor microenvironment, encompassing processes such as cell–cell recognition, binding, and adhesion. However, the function of immunoglobulin superfamily (IGSF) genes in tumor immune microenvironment remains uncharacterized. This study quantified the immune landscape by leveraging a gene expression matrix from publicly accessible databases. The associations between IGSF6 gene expression and immune cell infiltration were assessed. The expression levels of IGSF6, CD8+ T cells, CD4+ T cells and CD68+ macrophage cells in cancer tissues from CRC patients and CRC cell lines were evaluated. IGSF6 was more highly expressed in CRC tumor tissues than adjacent normal tissues. And IGSF6 was significantly correlated with immune cell infiltration in MMR-proficient patients. Remarkably, MMR-proficient patients with high IGSF6 expression showed more sensitive to immunotherapy and chemotherapy than those with low IGSF6 expression. In summary, IGSF6 could be a novel biomarker to evaluate immune infiltration and predict therapeutic effect for MMR-proficient CRC.
Sulphides from garlic essential oil dose-dependently change the distribution of glycerophospholipids and induce N6-tuberculosinyladenosine formation in mycobacterial cells
Authors: Sawicki Rafał , Widelski Jarosław , Truszkiewicz Wiesław , Kawka Sławomir , Kai Guoyin , Sieniawska Elwira
Published: November 2023

The antimicrobial properties of garlic are widely known, and numerous studies confirmed its ability to inhibit the growth of Mycobacterium tuberculosis. In this work, we explored the molecular mechanism of action of sulphides present in garlic essential oil against mycobacteria. The targeted transcriptomics and untargeted LC–MS metabolomics were applied to study dose- and time-dependent metabolic changes in bacterial cells under the influence of stressing agent. Expression profiles of genes coding stress-responsive sigma factors regulatory network and metabolic observations proved that sulphides from garlic essential oil are an efficient and specific agent affecting glycerophospholipids levels and their distribution within the cell envelope. Additionally, sulphides induced the Dimroth rearrangement of 1-Tuberculosinyladenosine to N6-tuberculosinyladenosine in mycobacterial cells as a possible neutralization mechanism protecting the cell from a basic nucleophilic environment. Sulphides affected cell envelope lipids and formation of N6-tuberculosinyladenosine in M. tuberculosis.
Effect of novel botanical synergist on the effectiveness and residue behavior of prothioconazole in wheat field
Authors: Wu Yalin , Yin Yuanjian , Chen Xin , Zhou Yeping , Jiang Shan , Zhang Mingming , Cai Guangcheng , Gao Quan
Published: November 2023

Fusarium head blight (FHB) is a critical fungal disease causes serious grain yield losses and mycotoxin contaminations. Currently, utilization of chemical fungicides is the main control method which has led to serious resistance. Development of novel synergist is an important strategy to reduce the usage of chemical fungicides and postpone the development of resistance, while natural components are interesting resources. In this study, the synergistic effect of Taxodium 'zhongshansha' essential oil (TZEO) was determined and the best synergistic ratio (SR) of 3.96 in laboratory which was observed when the weight ratio of TZEO and prothioconazole was 1 : 1 with the corresponding EC50 (half maximal effective concentration) value of Fusarium graminearum was 0.280 mg L−1. Subsequently, an increase of 6.31% on the control effect to FHB index in field test was observed when compared to the treatment with prothioconazole alone, though there was no significant difference between these treatments. Furthermore, we established an effective method to detect the mycotoxin contaminations in wheat grain with the limits of quantifications (LOQs) value of 5 µg kg−1 (DON, ZEN, 3-DON, and 15-DON) and 1 µg kg−1 (OTA) and the contents were less to the maximum residue limit (MRL) values. It was also shown that the application of 20% TZEO EW led to a 20% reduction in the use of prothioconazole, which was calculated based on the control effect values of 86.41% and 90.20% between the treatments of 30% prothioconazole OD (225 g a.i ha−1, recommend dosage) and 30% prothioconazole OD (180 g a.i ha−1) + 20% TZEO EW (225 mL ha−1), significantly. The initial residue of prothioconazole and prothioconazole-desthio was increased in the treatment with TZEO, which may play an important role in the synergistic effect on FHB. Moreover, none of the treatments posed a prothioconazole residue risk in the wheat grain and the environment. In addition, the essential oil has no any negative influence on wheat growth, which was revealed by a study of the chlorophyll content. These results provide an important botanical synergist for use with prothioconazole to control Fusarium head blight, and in-depth study to the synergistic mechanism of this oil is necessary in our future research.
Habitat selection of resident and non-resident gray wolves: implications for habitat connectivity
Authors: van den Bosch M. , Kellner K. F. , Gantchoff M. G. , Patterson B. R. , Barber-Meyer S. M. , Beyer D. E. , Erb J. D. , Isaac E. J. , MacFarland D. M. , Moore S. A. , Norton D. C. , Petroelje T. R. , Price Tack J. L. , Roell B. J. , Schrage M. , Belant J. L.
Published: November 2023

Habitat selection studies facilitate assessing and predicting species distributions and habitat connectivity, but habitat selection can vary temporally and among individuals, which is often ignored. We used GPS telemetry data from 96 Gray wolves (Canis lupus) in the western Great Lakes region of the USA to assess differences in habitat selection while wolves exhibited resident (territorial) or non-resident (dispersing or floating) movements and discuss implications for habitat connectivity. We used a step-selection function (SSF) to assess habitat selection by wolves exhibiting resident or non-resident movements, and modeled circuit connectivity throughout the western Great Lakes region. Wolves selected for natural land cover and against areas with high road densities, with no differences in selection among wolves when resident, dispersing, or floating. Similar habitat selection between resident and non-resident wolves may be due to similarity in environmental conditions, when non-resident movements occur largely within established wolf range rather than near the periphery or beyond the species range. Alternatively, non-resident wolves may travel through occupied territories because higher food availability or lower human disturbance outweighs risks posed by conspecifics. Finally, an absence of differences in habitat selection between resident and non-resident wolf movements may be due to other unknown reasons. We recommend considering context-dependency when evaluating differences in movements and habitat use between resident and non-resident individuals. Our results also provide independent validation of a previous species distribution model and connectivity analysis suggesting most potential wolf habitat in the western Great Lakes region is occupied, with limited connectivity to unoccupied habitat.
Topological magnetic structure generation using VAE-GAN hybrid model and discriminator-driven latent sampling
Authors: Park S. M. , Yoon H. G. , Lee D. B. , Choi J. W. , Kwon H. Y. , Won C.
Published: November 2023

Recently, deep generative models using machine intelligence are widely utilized to investigate scientific systems by generating scientific data. In this study, we experiment with a hybrid model of a variational autoencoder (VAE) and a generative adversarial network (GAN) to generate a variety of plausible two-dimensional magnetic topological structure data. Due to the topological properties in the system, numerous and diverse metastable magnetic structures exist, and energy and topological barriers separate them. Thus, generating a variety of plausible spin structures avoiding those barrier states is a challenging problem. The VAE-GAN hybrid model can present an effective approach to this problem because it brings the advantages of both VAE’s diversity and GAN’s fidelity. It allows one to perform various applications including searching a desired sample from a variety of valid samples. Additionally, we perform a discriminator-driven latent sampling (DDLS) using our hybrid model to improve the quality of generated samples. We confirm that DDLS generates various plausible data with large coverage, following the topological rules of the target system.
EMT-related gene risk model establishment for prognosis and drug treatment efficiency prediction in hepatocellular carcinoma
Authors: Gao Xiaqing , Yang Chunting , Li Hailong , Shao Lihua , Wang Meng , Su Rong
Published: November 2023

This study was designed to evaluate the prognosis and pharmacological therapy sensitivity of epithelial mesenchymal transition-related genes (EMTRGs) that obtained from the EMTome database in hepatocellular carcinoma (HCC) using bioinformatical method. The expression status of EMTRGs were also investigated using the clinical information of HCC patients supported by TCGA database and the ICGC database to establish the TCGA cohort as the training set and the ICGC cohort as the validation set. Analyze the EMTRGs between HCC tissue and liver tissue in the TCGA cohort in the order of univariate COX regression, LASSO regression, and multivariate COX regression, and construct a risk model for EMTRGs. In addition, enrichment pathways, gene mutation status, immune infiltration, and response to drugs were also analyzed in the high-risk and low-risk groups of the TCGA cohort, and the protein expression status of EMTRGs was verified. The results showed a total of 286 differentially expressed EMTRGs in the TCGA cohort, and EZH2, S100A9, TNFRSF11B, SPINK5, and CCL21 were used for modeling. The TCGA cohort was found to have a worse outcome in the high-risk group of HCC patients, and the ICGC cohort confirmed this finding. In addition, EMTRGs risk score was shown to be an independent prognostic factor in both cohorts by univariate and multivariate COX regression. The results of GSEA analysis showed that most of the enriched pathways in the high-risk group were associated with tumor, and the pathways enriched in the low-risk group were mainly associated with metabolism. Patients in various risk groups had varying immunological conditions, and the high-risk group might benefit more from targeted treatments. To sum up, the EMTRGs risk model was developed to forecast the prognosis for HCC patients, and the model might be useful in assisting in the choice of treatment drugs for HCC patients.
Radiological artificial intelligence - predicting personalized immunotherapy outcomes in lung cancer
Authors: Roisman Laila C. , Kian Waleed , Anoze Alaa , Fuchs Vered , Spector Maria , Steiner Roee , Kassel Levi , Rechnitzer Gilad , Fried Iris , Peled Nir , Bogot Naama R.
Published: November 2023

Personalized medicine has revolutionized approaches to treatment in the field of lung cancer by enabling therapies to be specific to each patient. However, physicians encounter an immense number of challenges in providing the optimal treatment regimen for the individual given the sheer complexity of clinical aspects such as tumor molecular profile, tumor microenvironment, expected adverse events, acquired or inherent resistance mechanisms, the development of brain metastases, the limited availability of biomarkers and the choice of combination therapy. The integration of innovative next-generation technologies such as deep learning—a subset of machine learning—and radiomics has the potential to transform the field by supporting clinical decision making in cancer treatment and the delivery of precision therapies while integrating numerous clinical considerations. In this review, we present a brief explanation of the available technologies, the benefits of using these technologies in predicting immunotherapy response in lung cancer, and the expected future challenges in the context of precision medicine.
Genetic evidence for differential functions of figla and nobox in zebrafish ovarian differentiation and folliculogenesis
Authors: Wu Kun , Zhai Yue , Qin Mingming , Zhao Cheng , Ai Nana , He Jianguo , Ge Wei
Published: November 2023

FIGLA and NOBOX are important oocyte-specific transcription factors. Both figla-/- and nobox-/- mutants showed all-male phenotype in zebrafish due to increased dominance of the male-promoting pathway. The early diversion towards males in these mutants has precluded analysis of their roles in folliculogenesis. In this study, we attenuated the male-promoting pathway by deleting dmrt1, a key male-promoting gene, in figla-/- and nobox-/- fish, which allows a sufficient display of defects in folliculogenesis. Germ cells in figla-/-;dmrt1-/- double mutant remained in cysts without forming follicles. In contrast, follicles could form well but exhibited deficient growth in nobox-/-;dmrt1-/- double mutants. Follicles in nobox-/-;dmrt1-/- ovary could progress to previtellogenic (PV) stage but failed to enter vitellogenic growth. Such arrest at PV stage suggested a possible deficiency in estrogen signaling. This was supported by lines of evidence in nobox-/-;dmrt1-/-, including reduced expression of ovarian aromatase (cyp19a1a) and level of serum estradiol (E2), regressed genital papilla (female secondary sex characteristics), and more importantly the resumption of vitellogenic growth by E2 treatment. Expression analysis suggested Nobox might regulate cyp19a1a by controlling Gdf9 and/or Bmp15. Our discoveries indicate that Figla is essential for ovarian differentiation and follicle formation whereas Nobox is important for driving subsequent follicle development.
A common pathway for detergent-assisted oligomerization of Aβ42
Authors: Muhammedkutty Fidha Nazreen Kunnath , Prasad Ramesh , Gao Yuan , Sudarshan Tarunya Rao , Robang Alicia S. , Watzlawik Jens O. , Rosenberry Terrone L. , Paravastu Anant K. , Zhou Huan-Xiang
Published: November 2023

Amyloid beta (Aβ) aggregation is a slow process without seeding or assisted nucleation. Sodium dodecyl sulfate (SDS) micelles stabilize Aβ42 small oligomers (in the dimer to tetramer range); subsequent SDS removal leads to a 150-kD Aβ42 oligomer. Dodecylphosphorylcholine (DPC) micelles also stabilize an Aβ42 tetramer. Here we investigate the detergent-assisted oligomerization pathway by solid-state NMR spectroscopy and molecular dynamics simulations. SDS- and DPC-induced oligomers have the same structure, implying a common oligomerization pathway. An antiparallel β-sheet formed by the C-terminal region, the only stable structure in SDS and DPC micelles, is directly incorporated into the 150-kD oligomer. Three Gly residues (at positions 33, 37, and 38) create holes that are filled by the SDS and DPC hydrocarbon tails, thereby turning a potentially destabilizing feature into a stabilizing factor. These observations have implications for endogenous Aβ aggregation at cellular interfaces.
Taxonomic identification and temperature stress tolerance mechanisms of Aequorivita marisscotiae sp. nov
Authors: Liu Wenqi , Cong Bailin , Lin Jing , Liu Shenghao , Deng Aifang , Zhao Linlin
Published: November 2023

The deep sea harbours microorganisms with unique life characteristics and activities due to adaptation to particular environmental conditions, but the limited sample collection and pure culture techniques available constrain the study of deep-sea microorganisms. In this study, strain Ant34-E75 was isolated from Antarctic deep-sea sediment samples and showed the highest 16 S rRNA gene sequence similarity (97.18%) with the strainAequorivita viscosa8-1bT. Strain Ant34-E75 is psychrotrophic and can effectively increase the cold tolerance ofChlamydomonas reinhardtii(a model organism). Subsequent transcriptome analysis revealed multiple mechanisms involved in the Ant34-E75 response to temperature stress, and weighted gene co-expression network analysis (WGCNA) showed that the peptidoglycan synthesis pathway was the key component. Overall, this study provides insights into the characteristics of a deep-sea microorganism and elucidates mechanisms of temperature adaptation at the molecular level.
Breakdown of conventional winding number calculation in one-dimensional lattices with interactions beyond nearest neighbors
Authors: Rajabpoor Alisepahi Amir , Sarkar Siddhartha , Sun Kai , Ma Jihong
Published: November 2023

Topological insulators hold promises to realize exotic quantum phenomena in electronic, photonic, and phononic systems. Conventionally, topological indices, such as winding numbers, have been used to predict the number of topologically protected domain-wall states (TPDWSs) in topological insulators, a signature of the topological phenomenon called bulk-edge correspondence. Here, we demonstrate theoretically and experimentally that the number of TPDWSs in a mechanical Su-Schrieffer-Heeger (SSH) model can be higher than the winding number depending on the strengths of beyond-nearest-neighbor interactions, revealing the breakdown of the winding number prediction. Alternatively, we resort to the Berry connection to accurately characterize the number and spatial features of TPDWSs in SSH systems, further confirmed by the Jackiw-Rebbi theory proving that the multiple TPDWSs correspond to the bulk Dirac cones. Our findings deepen the understanding of complex network dynamics and offer a generalized paradigm for precise TPDWS prediction in potential applications involving localized vibrations, such as drug delivery and quantum computing.
Magnetic mesoporous Fe3O4@nSiO2@mSiO2 nanoparticles for high-throughput mass spectrometry detection of hydrolyzed products of organophosphorus nerve agents
Authors: Qu Gang , Zhao Yuxin , Zhang Qiaoli , Wu Jina , Li Xiaosen , Yang Yang , Liu Shilei
Published: November 2023

A QuEChERS method based on a novel magnetic nanomesoporous material was developed for the efficient extraction of methyl phosphonates in the environment and the analysis of 70 environmental samples could be completed within 20 min.
Drug delivery nanoparticles for preventing implant bacterial infections based on the bacteria and immunity mechanisms
Authors: Chen Chen , Xiao Qi , Xiao Leyi , Feng Mengge , Liu Fangzhe , Yao Ke , Cui Yu , Zhang Tiange , Zhang Yufeng
Published: November 2023

Diagram of ICG+RSG effects: ICG+RSG was irradiated by 808 nm laser to release ROS and elevate the temperature for bacterial killing. ICG+RSG promoted M2 polarization and enhance macrophage phagocytosis to improve the anti-inflammatory responses.
Application of molecularly imprinted polymers (MIPs) as environmental separation tools
Authors: Gkika Despina A. , Tolkou Athanasia K. , Lambropoulou Dimitra A. , Bikiaris Dimitrios N. , Kokkinos Petros , Kalavrouziotis Ioannis K. , Kyzas George Z.
Published: November 2023

This review is structured to outline the applications of MIPs as environmental separation tools and assess the role of functional groups as a parameter influencing MIP performance.
A review on zirconium-based metal–organic frameworks: synthetic approaches and biomedical applications
Authors: Chattopadhyay Krishna , Mandal Manas , Maiti Dilip Kumar
Published: November 2023

Zr-MOFs, known for stability, low cytotoxicity and high drug-loading capacity; have been thoroughly investigated for biomedical uses. This article reviews the various synthetic methods and recent biomedical applications of important Zr-based MOFs.
Development of immunoliposomes containing cytotoxic gold payloads against HER2-positive breast cancers
Authors: Ahad Afruja , Aftab Fatima , Michel Alexa , Lewis Jason S. , Contel Maria
Published: November 2023

HER2-targeted immunoliposomes with gold payloads exhibit greater accumulation than non-targeted liposomes and free gold compounds and localize in the mitochondria and endoplasmic reticulum leading to cell death at lower nanomolar drug concentrations.
Naphthyl bearing 1,3,4-thiadiazoleacetamides targeting the parasitic folate pathway as anti-infectious agents: in silico, synthesis, and biological approach
Authors: Pal Kavita , Lala Sahil , Agarwal Priyanka , Patel Tarosh S. , Legac Jenny , Rahman Md. Ataur , Ahmedi Saiema , Shahid Nida , Singh Sneha , Kumari Kajal , Madhav Hari , Sen Abhik , Manzoor Nikhat , Dixit Bharat C. , Van Zyl Robyn , Rosenthal Philip J. , Hoda Nasimul
Published: November 2023

Naphthyl bearing 1,3,4-thiadiazoleacetamides targeting the parasitic folate pathway as antimalarials and antileishmanial agents.
High-performance sono-piezoelectric nanocomposites enhanced by interfacial coupling effects for implantable nanogenerators and actuators
Authors: Chen Yingxin , Shi Jingchao , Yang Guowei , Zhu Ning , Zhang Lei , Yang Dexin , Yao Ni , Zhang Wentao , Li Yongshuang , Guo Qiyun , Wang Yuxiang , Wang Yan , Yang Tao , Liu Xiaolian , Zhang Jian
Published: November 2023

High-performance implantable and wireless P(VDF-CTFE)/BT@Carbon piezoelectric nanogenerators are developed for harvesting ultrasound for biological device power supply.
Microelectromechanical system for in situ quantitative testing of tension–compression asymmetry in nanostructures
Authors: Huang Yuheng , Yin Kuibo , Li Binghui , Zheng Anqi , Wu Bozhi , Sun Litao , Nie Meng
Published: November 2023

A novel on-chip microelectromechanical system (MEMS) is used for in situ quantitative testing of asymmetric mechanical properties of nanostructures, with potential applications in nanotechnology research.
Evidence for an L3 phase in ternary deep eutectics: composition-induced L3-to-Lα transition of AOT
Authors: Hammond Oliver S. , Elstone Naomi S. , Doutch James , Li Peixun , Edler Karen J.
Published: November 2023

Small-angle neutron scattering (SANS) shows that the ternary DES choline chloride–urea–water has no nanostructure. However, adding the di-chain surfactant AOT promotes L3 or Lα phases, controllable via water content.
An ultra-broadband frequency-agile terahertz perfect absorber with perturbed MoS2 plasmon modes
Authors: Zhong Yujie , Huang Yi , Zhong Shuncong , Shi Tingting , Sun Fuwei , Lin Tingling , Zeng Qiuming , Yao Ligang , Chen Xuefeng
Published: November 2023

The prism-coupled absorber with electrically-driven MoS2 excites perturbed plasmon modes to induce susceptible dielectric response and unique dual phase singularities, which achieve active multidomain manipulations in an ultra-broadband range.
Dual-wavelength responsive CuS@COF nanosheets for high-performance photothermal/photodynamic combination treatments
Authors: An Qian , Su Shengze , Hu Wei , Wang Yanying , Liang Tao , Li Xianghong , Li Chunya
Published: November 2023

CuS@COF nanosheets were employed for high-performance photothermal/photodynamic combination treatments as being alternately illuminated with 505 nm and 1064 nm laser.
K2S2O8-mediated direct C–H heteroarylation/hydroxylation of indolin-2-ones with quinoxalin-2(1H)-ones
Authors: Qin Hui , Wei Guoliang , Lou Yutao , Zheng Xiaowei , Bao Meihua , Zhang Yiwen , Huang Ping
Published: November 2023

A series of 3-heteraryl-3-hydroxyindoles are obtained via an oxidative cross-coupling reaction between quinoxalin-2(1H)-ones and indolin-2-ones in low-to-good yields.
Poly(hexamethylene biguanide) hydrochloride (PHMB)-based materials: synthesis, modification, properties, determination, and application
Authors: Wang Wen-Yi , Hu Hua-Wen , Chiou Jia-Chi , Yung Ka-Fu , Kan Chi-Wai
Published: November 2023

Conceptual diagram of the current review covering the synthesis, modification, properties, determination, and applications of PHMB-based materials.
Covalent shaping of polyoxometalate molecular films onto ITO electrodes for charge trapping induced resistive switching
Authors: Salles Raphaël , Poh Wei Church , Laurans Maxime , Volatron Florence , Miche Antoine , Alves Sandra , Carino Christian , Tortech Ludovic , Izzet Guillaume , Lee Pooi See , Proust Anna
Published: November 2023

A few nanometers thick film of polyoxometalates has been covalently assembled onto ITO for resistive switching.
Molybdate ion leaching and re-adsorption facilitate structural and electronic modulation of nickel–iron catalysts for seawater electrolysis
Authors: Zhou Jieshu , Liu Lihua , Ren Hangxing , Li Liming , Song Wei , Li Ning , Shi Xuxia , Kou Chendong , Sun Yuetao , Han Mei , Wang Haibin , Han Jingrui , Liu Kangning , Momo Christopher Dorma , Liu Yingying , Feng Donghui , Zhu Weijun , Chen Songhua , Jiang Hui , Liu Yongchang , Liang Hongyan
Published: November 2023

Continuous leaching of MoO42− accelerates the collapse of the host structure and generation of NiFeOOH. The re-adsorbed MoO42− modify the electronic environment of the Ni and Fe sites and modulates their d-band centers, boosting the OER kinetics.
Towards separator safety of lithium-ion batteries: a review
Authors: Tong Boli , Li Xifei
Published: November 2023

The recent advance of high-safety separators with high mechanical strength, high thermal stability and good lithium dendritic resistance is the main focus in this review. The future challenges and perspectives of separators are provided for building high safety rechargeable lithium batteries.
The transient covering of iridium species with ultrathin carbon shells via Joule-heating for robust acidic water oxidation
Authors: Guan Zeyu , Li Jiankun , Li Shiyi , Wang Keyu , Lei Linfeng , Wang Yixing , Zhuang Linzhou , Xu Zhi
Published: November 2023

The rapid high heat input and precise energy control are crucial for transient carbon encapsulation, which could be realized by the Joule-heating treatment. Iridium species with carbon encapsulation can stably operate in oxygen evolution catalysis.
Transition-metal catalyzed reactions of diazo compounds and N,N-dialkylnitrosoamines
Authors: Chen Yuan , Peng Rui-jun , Zhang Xue-jing , Yan Ming
Published: November 2023

Transition-metal catalyzed reactions of diazo compounds and N,N-dialkylnitrosoamines had been developed, leading to the efficient preparation of isoquinoline-1,3,4-trione derivatives or nitroso ylide products in moderate to excellent yields.
N-Aminophthalimide-mediated aerobic deborohydroxylation of boronic acid in air
Authors: Chen Tao , Zhang Wenzheng , Yan Zhenxing , Xu Ze-Feng , Li Chuan-Ying
Published: November 2023

Using readily available N-aminophthalimide as a mediator to activate molecular oxygen, an efficient and convenient metal-free aerobic deborohydroxylation of boronic acids into phenols and alcohols in air is reported.
Electrochemical synthesis of CN-substituted imidazo[1,5-a]pyridines via a cascade process using NH4SCN as both an electrolyte and a non-trivial cyanating agent
Authors: Grishin Sergei S. , Mulina Olga M. , Vil’ Vera A. , Terent'ev Alexander O.
Published: November 2023

An electrochemical cascade of oxidation/cyclization of pyridine-2-carboxaldehydes with benzylamines involving in situ cyanation with NH4SCN to afford 1-cyano-imidazo[1,5-a]pyridines was developed.
Interception of RCONCl2: late-stage hydrolysis and esterification of primary amides
Authors: Wan Xiao , Lin Jiaxin , Bai Junjie , Li Jingjing , Wang Kaifeng , Cao Zhiyu , Li Minggang , Bao Xiaoguang , Wan Xiaobing
Published: November 2023

Through the interception of RCONCl2, a versatile method for hydrolysis and esterification of primary amides has been well developed.
Green structure orienting and reducing agents of wheat peel extract induced abundant surface oxygen vacancies and transformed the nanoflake morphology of NiO into a plate-like shape with enhanced non-enzymatic urea sensing application
Authors: Parveen Mehnaz , Tahira Aneela , Mahar Ihsan Ali , Bhatti Muhammad Ali , Dawi Elmuez , Nafady Ayman , Alshammari Riyadh H. , Vigolo Brigitte , Qi Kezhen , Ibupoto Zafar Hussain
Published: November 2023

Researchers are increasingly focusing on using biomass waste for green synthesis of nanostructured materials since green reducing, capping, stabilizing and orientation agents play a significant role in final application.
Quantitative detection of microRNA-21 in vivo using in situ assembled photoacoustic and SERS nanoprobes
Authors: Zheng Liting , Li Qingqing , Wu Ying , Su Lichao , Du Wei , Song Jibin , Chen Lanlan , Yang Huanghao
Published: November 2023

A miR-21-activated self-assembled AuNNP@AuNP core–satellite was developed to quantify the absolute concentration of miR-21 via enhanced photoacoustic and ratiometric SERS signals in vivo for the first time.
Heat-transfer enhancement and optimization design of a roadway with typical angles using field synergy theory
Authors: Qu Min , Zhang Yongliang , Zhang Xilong , Li Mingjie , Liu Yunfei , Hu Zhen
Published: November 2023

In underground excavation operations, an excavation path with a turning radius of 3.5 meters should be selected which has the best comprehensive performance.
Powerful drying and doping strategies for enhancing the thermoelectric performance of tellurium nanostructures prepared via green hydrothermal synthesis
Authors: Kim In Ho , Jeong Yong Jin
Published: November 2023

An effective freeze-drying and solution-based In doping approach was developed to enhance the thermoelectric properties of Te nanostructures via green hydrothermal synthesis.
Effect of chloride salts and microwaves on polyethylene terephthalate (PET) hydrolysis by iron chloride/acetic acid Lewis/Brønsted acidic deep eutectic solvent
Authors: Rollo Marco , Perini Massimo A. G. , Sanzone Alessandro , Polastri Lorenzo , Tiecco Matteo , Torregrosa-Chinillach Alejandro , Martinelli Elisa , Ciancaleoni Gianluca
Published: November 2023

Iron(iii)-based deep eutectic solvents were demonstrated to be active solvents for the hydrolysis of poly(ethylene terephthalate), especially under microwave irradiation.
An improved Hummers method derived graphene oxide wrapped ZIF-8 polyhedron derived porous heterostructure for symmetric supercapacitor performance
Authors: Patil Rahul , Kumar Nitish , Matsagar Babasahab , Wu Kevin C. W. , Salunkhe Rahul R. , Dutta Saikat
Published: November 2023

By exposing more interfaces, utility of heterostructured electrodes in capacitance retention has been further expanded to devices with a large-scale 3D network. IGO@ZIF-8-NC was described as a symmetric supercapacitor device which paves the way forward for sustainable capacitive devices.
Fast Na-diffusive tin alloy for all-solid-state Na-based batteries
Authors: Tanibata Naoto , Matsunoshita Koki , Takeuchi Hirokazu , Akatsuka Suzuno , Koga Misato , Takeda Hayami , Nakayama Masanobu
Published: November 2023

A metastable-phase Na–Sn binary alloy exhibiting exceptionally high Na diffusivity at room temperature (D25 = 5.4 × 10−7 cm2 s−1) was applied to a next-generation all-solid-state sodium battery, achieving ultralow energy loss properties.
New insights into the synthesis of Sillén–Aurivillius oxyhalides: molten salts induce interlayer halogen competing reaction
Authors: Zhang Yunxiang , Zhou Chenliang , Xu Shishi , Abdelsalam Hazem , Mu Zhichao , Chen Wei , Chen Zhili , Cheng Xiangyu , Khalafallah Diab , Zhang Qinfang
Published: November 2023

The molten-salt synthesis method offers a special liquid reaction environment that expedites the diffusion of reactive elements and provides the competing reaction of the migrated halogen atoms during the synthesis of Sillén–Aurivillius oxyhalides.
Characterization and quantification of microplastics and organic pollutants in mussels by microwave-assisted sample preparation and analytical pyrolysis
Authors: Biale Greta , La Nasa Jacopo , Fiorentini Lorenzo , Ceccarini Alessio , Carnaroglio Diego , Mattonai Marco , Modugno Francesca
Published: November 2023

We developed a method to isolate and quantify various classes of both non-polymeric contaminants and polymers from mussel flour using microwave-assisted extraction/digestion and analytical pyrolysis-GC-MS.
An expansion planning model for strategic visioning of active distribution network in the presence of local electricity market
Authors: Solat Sajjad , Aminifar Farrokh , Safdarian Amir , Shayanfar Heidarali
Published: November 2023

Emergence of different stakeholders with contrary objectives in active distribution network (ADN) requires for a fair network expansion planning that considers all of the contrary objectives. This paper provides an expansion planning model for ADN strategic visioning from electric distribution stakeholders' organization (EDSO) perspective, which takes into account the objectives of all stakeholders. The stakeholders are network owner, active customers (ACs), consumers, and environmental regulatory body (ERB). The proposed model is multistage bi‐level, where the upper‐level minimizes the present value of the total costs imposed to all ADN stakeholders and the lower‐level minimizes the total generation costs within the distribution‐level electricity market. The Karush–Kuhn–Tucker optimality conditions and the linearization techniques are employed through which the model is converted to mixed integer linear programming which can be tackled effectively. The model provides a visionary expansion plan that policymaker must legislate to realize it. Effectiveness of the model in providing a visionary expansion plan considering EDSO's perspective in making cost effective planning decisions per social welfare goals is verified through several case studies on 33‐bus standard test system. According to the simulation results, transition from DSO to EDSO in the presence of low‐capacity ACs improves social welfare by 5%. But this improvement is up to 70% in the presence of high‐capacity ACs.