bluuebunny/crossref_metadata_2025 huggingface dataset:
Regulation of ErbB Receptors by the Ca2+ Sensor Protein Calmodulin in Cancer
Authors: Villalobo Antonio
Published: February 2023

Overexpression and mutations of the epidermal growth factor receptor (EGFR/ErbB1/HER1) and other tyrosine kinase receptors of the ErbB family (ErbB2/HER2, ErbB3/HER3 and ErbB4/HER4) play an essential role in enhancing the proliferation, the migratory capacity and invasiveness of many tumor cells, leading to cancer progression and increased malignancy. To understand these cellular processes in detail is essential to understand at a molecular level the signaling pathways and regulatory mechanisms controlling these receptors. In this regard, calmodulin (CaM) is a Ca2+-sensor protein that directly interacts with and regulates ErbB receptors, as well as some CaM-dependent kinases that also regulate these receptors, particularly EGFR and ErbB2, adding an additional layer of CaM-dependent regulation to this system. In this short review, an update of recent advances in this area is presented, covering the direct action of Ca2+/CaM on the four ErbB family members mostly in tumor cells and the indirect action of Ca2+/CaM on the receptors via CaM-regulated kinases. It is expected that further understanding of the CaM-dependent mechanisms regulating the ErbB receptors in future studies could identify new therapeutic targets in these systems that could help to control or delay cancer progression.
Improved Stability and Hydrolysates of Hyperthermophilic GH57 Type II Pullulanase from the Deep-Sea Archaeon Thermococcus siculi HJ21 by Truncation
Authors: Wu Xudong , Dou Baojie , Wang Boyan , Liu Mingwang , Shao Ruxue , Lu Jing , Lyu Mingsheng , Wang Shujun
Published: February 2023

Pullulanase (EC 3.2.1.41) belongs to the amylase family and is often used alone or in combination with other amylases in the industrial production of starch-based products. This enzyme is often required in industrial production because of its better stability. We here truncated the pullulanase gene from the deep-sea hydrothermal anaerobic archaeon Thermococcus siculi HJ21 and obtained Pul-HJΔ782, which is a member of the α-amylase family GH57. The results revealed that the optimum temperature for Pul-HJΔ782 was 100 °C, and its thermostability at 100 °C improved after truncation. Less than 15% of its enzyme activity was lost after 1 h of incubation at 100 °C, and 57% activity remained after 5 h of treatment. Truncation significantly improved the overall pH tolerance range of Pul-HJΔ782, and its stability in the pH range 4–8 was over 80% relative activity from an average of 60%. The sequence and structural model of Pul-HJΔ782 was analyzed, and its instability index was reduced significantly. Furthermore, the hydrolysates of the truncated and wild-type pullulanase were analyzed, and the enzymatic digestion efficiency of the truncated Pul-HJΔ782 was higher.
Photoelectrochemical Conversion of Sewage Water into H2 Fuel over the CuFeO2/CuO/Cu Composite Electrode
Authors: Hadia N. M. A. , Shaban Mohamed , Ahmed Ashour M. , Mohamed W. S. , Alzaid Meshal , Ezzeldien Mohammed , Hasaneen M. F. , El Malti Wassim , Abdelazeez Ahmed Adel A. , Rabia Mohamed
Published: February 2023

This study describes the synthesis of delafossite, CuFeO2, as a primary photocatalytic material for hydrogen generation. A photoelectrode, CuFeO2/CuO/Cu, was prepared by combusting a Cu foil dipped in FeCl3 in ambient air. This photoelectrode showed excellent optical behavior for the hydrogen generation reaction from sewage water, producing 90 µmol/h of H2. The chemical structure was confirmed through XRD and XPS analyses, and the crystalline rhombohedral shape of CuFeO2 was confirmed using SEM and TEM analyses. With a bandgap of 1.35 ev, the prepared material displayed excellent optical properties. Electrochemical measurements for H2 gas generation were carried out using the CuFeO2/CuO/Cu photoelectrode, comparing the effect of light and dark and monochromatic wavelength light. The electrode exhibited significant enhancement in light compared to dark, with current density (Jph) values of −0.83 and −0.1 mA·cm−2, respectively. The monochromatic light also had a noticeable effect, with the Jph value increasing from −0.45 to −0.79 mA·cm−2 as the wavelength increased from 640 to 390 nm. This system is cheap and durable, making it a promising solution for hydrogen gas fuel generation in the industry.
The Quick Removal of Toxic Dye Molecules by an Efficient Adsorptive BiOI/Bi2MoO6 Heterostructure
Authors: Onyedika Tasie Ebenezer , Xu Mengying , Deng Yichao , Liu Yang , Li Lian , Tremblay Pier-Luc , Zhang Tian
Published: February 2023

Adsorption is a low-energy, economical, and efficient method for pollutant removal from water. Because of their unique structure, large specific surface area (SSA), and non-toxicity, bismuth-based semiconductors, usually researched for the photodegradation of organic molecules, are also excellent for dark adsorption processes. Here, a three-dimensional adsorbent with a heterostructure with a hydrangea-like shape made of Bi2MoO6 (BMO) and BiOI (BOI) was synthesized by a one-pot solvothermal process and investigated for the adsorption of toxic dyes. BOI/BMO with an I-to-Mo ratio of 2.0 adsorbed 98.9% of the model pollutant rhodamine B (RhB) within 5 min with a maximum adsorption capacity of 72.72 mg/g in the dark at room temperature. When compared to pure BMO, the BOI2/BMO heterostructure was 14.1 times more performant because of its flower-like morphology with multiple planes, an SSA that was 1.6-fold larger, increased porosity, the formation of heterojunctions, and a negative surface charge attracting RhB. Further investigation indicated that adsorption by BOI2/BMO fitted the pseudo-second-order kinetic and the Langmuir isotherm models. In addition, the thermodynamic analysis showed that it was a spontaneous exothermic process probably relying on physisorption. Thus, the BOI/BMO adsorbent developed here is promising for the fast removal of toxic dyes from industrial wastewater.
One Stone Two Birds: Utilization of Solar Light for Simultaneous Selective Phenylcarbinol Oxidation and H2 Production over 0D/2D-3D Pt/In2S3 Schottky Junction
Authors: Zhang Huijun , Xiao Peipei , Meng Sugang , Long Baihua , Liu Qing , Zheng Xiuzhen , Zhang Sujuan , Ruan Zhaohui , Chen Shifu
Published: February 2023

Precise regulation and control solar-light-driven charges photoexcited on photocatalysts for separation-transfer and target redox reactions is an attractive and challenging pathway toward sustainability. Herein, 0D/2D-3D Pt/In2S3 Schottky junction was fabricated for simultaneous selective phenylcarbinol conversion into value-added aldehydes and production of clean energy H2 by directly utilizing photoexcited holes and electrons in one reaction system under mild reaction conditions. In contrast to pure water splitting and pure In2S3, the reaction thermodynamics and kinetics of H2 evolution on the Pt/In2S3 were significantly enhanced. The optimized 0.3% Pt/In2S3 exhibited the highest and most stable photocatalytic activity with 22.1 mmol g−1 h−1 of H2 production rate and almost 100% selectivity of benzaldehyde production. Notably, this dual-function photocatalysis also exhibited superiority in contrast to sacrificial-agent H2 evolution reactions such as lactic acid, Na2S, methanol and triethanolamine. The turnover frequency (TOF) could reach up to ~2394 h−1. The Pt clusters anchored at the electron location and strong metal-support interactions (SMSI) between Pt and In2S3 synergistically improved the spatial charge separation and directional transportation (~90.1% of the charge transport efficiency could be achieved over the Pt/In2S3 hybrid), and thus result in significant enhancement of photocatalytic H2 evolution with simultaneous benzaldehyde production.
Proliferating Astrocytes in Primary Culture Do Not Depend upon Mitochondrial Respiratory Complex I Activity or Oxidative Phosphorylation
Authors: Silva Ellen A. , Dalla Costa Ana P. , Ruas Juliana S. , Siqueira-Santos Edilene S. , Francisco Annelise , Castilho Roger F.
Published: February 2023

Understanding the role of astrocytes in the development of the nervous system and neurodegenerative disorders implies a necessary knowledge of the oxidative metabolism of proliferating astrocytes. The electron flux through mitochondrial respiratory complexes and oxidative phosphorylation may impact the growth and viability of these astrocytes. Here, we aimed at assessing to which extent mitochondrial oxidative metabolism is required for astrocyte survival and proliferation. Primary astrocytes from the neonatal mouse cortex were cultured in a physiologically relevant medium with the addition of piericidin A or oligomycin at concentrations that fully inhibit complex I-linked respiration and ATP synthase, respectively. The presence of these mitochondrial inhibitors for up to 6 days in a culture medium elicited only minor effects on astrocyte growth. Moreover, neither the morphology nor the proportion of glial fibrillary acidic protein-positive astrocytes in culture was affected by piericidin A or oligomycin. Metabolic characterization of the astrocytes showed a relevant glycolytic metabolism under basal conditions, despite functional oxidative phosphorylation and large spare respiratory capacity. Our data suggest that astrocytes in primary culture can sustainably proliferate when their energy metabolism relies only on aerobic glycolysis since their growth and survival do not require electron flux through respiratory complex I or oxidative phosphorylation.
Mobile Cone-Beam CT-Assisted Bronchoscopy for Peripheral Lung Lesions
Authors: Salahuddin Moiz , Bashour Sami I. , Khan Asad , Chintalapani Gouthami , Kleinszig Gerhard , Casal Roberto F.
Published: February 2023

Peripheral bronchoscopy with the use of thin/ultrathin bronchoscopes and radial-probe endobronchial ultrasound (RP-EBUS) has been associated with a fair diagnostic yield. Mobile cone-beam CT (m-CBCT) could potentially improve the performance of these readily available technologies. We retrospectively reviewed the records of patients undergoing bronchoscopy for peripheral lung lesions with thin/ultrathin scope, RP-EBUS, and m-CBCT guidance. We studied the performance (diagnostic yield and sensitivity for malignancy) and safety (complications, radiation exposure) of this combined approach. A total of 51 patients were studied. The mean target size was 2.6 cm (SD, 1.3 cm) and the mean distance to the pleura was 1.5 cm (SD, 1.4 cm). The diagnostic yield was 78.4% (95 CI, 67.1–89.7%), and the sensitivity for malignancy was 77.4% (95 CI, 62.7–92.1%). The only complication was one pneumothorax. The median fluoroscopy time was 11.2 min (range, 2.9–42.1) and the median number of CT spins was 1 (range, 1–5). The mean Dose Area Product from the total exposure was 41.92 Gy·cm2 (SD, 11.35 Gy·cm2). Mobile CBCT guidance may increase the performance of thin/ultrathin bronchoscopy for peripheral lung lesions in a safe manner. Further prospective studies are needed to corroborate these findings.
Virtual Reality as the Catalyst for a Novel Partnership Model in Initial Teacher Education: ITE Subject Methods Tutors’ Perspectives on the Island of Ireland
Authors: Cowan Pamela , Farrell Rachel
Published: February 2023

This small-scale study explores the attitudes of fifty initial teacher education (ITE) subject methods tutors towards the use of virtual reality (VR) in education and considers whether VR could be a catalyst for reviewing the partnership model within ITE programmes. In addition, this study offers a novel solution to ITE tutors’ challenges when managing their own technological and pedagogical development alongside preparing student teachers for technology-enhanced learning (TEL). Building on previous research on ITE tutors’ use of TEL across the island of Ireland, this paper discusses the synergy between the cognitive apprenticeship model and reverse mentoring that upends the classic co-operating teacher/student teacher hierarchy and positions all partners in ITE programmes as learning leaders at key points in the partnership process. An online survey comprising 51 items was administered to a purposive sample of 50 ITE tutors from four ITE providers, two in Ireland and two in Northern Ireland (NI), who were selected due to their developing interest in the use of virtual reality in teacher education. Data analysis using SPSS combined with thematic analysis of open-ended responses revealed that although the majority of ITE subject methods tutors conveyed open-minded and willing attitudes to embrace VR in the future, they identified a number of systemic issues that need to be addressed first. These include the disconnect between innovative pedagogical practices presented in university-based modules and the stark reality of technological deficits in some classrooms; the pedagogical and resource-based ‘readiness’ of ITE tutors to embed VR in their subject-specific teaching; and the need for curriculum-focused, VR resources for school-based use. As a result of this study, an incremental, cyclical approach to growing the body of knowledge around VR pedagogy is proposed in conjunction with new forms of collaboration between the partners in ITE.
OCR Applied for Identification of Vehicles with Irregular Documentation Using IoT
Authors: Glasenapp Luiz Alfonso , Hoppe Aurélio Faustino , Wisintainer Miguel Alexandre , Sartori Andreza , Stefenon Stefano Frizzo
Published: February 2023

Given the lack of investments in surveillance in remote places, this paper presents a prototype that identifies vehicles in irregular conditions, notifying a group of people, such as a network of neighbors, through a low-cost embedded system based on the Internet of things (IoT). The developed prototype allows the visualization of the location, date and time of the event, and vehicle information such as license plate, make, model, color, city, state, passenger capacity and restrictions. It also offers a responsive interface in two languages: Portuguese and English. The proposed device addresses technical concepts pertinent to image processing such as binarization, analysis of possible characters on the plate, plate border location, perspective transformation, character segmentation, optical character recognition (OCR) and post-processing. The embedded system is based on a Raspberry having support to GPS, solar panels, communication via 3G modem, wi-fi, camera and motion sensors. Tests were performed regarding the vehicle’s positioning and the percentage of assertiveness in image processing, where the vehicles are at different angles, speeds and distances. The prototype can be a viable alternative because the results were satisfactory concerning the recognition of the license plates, mobility and autonomy.
Soy Protein Hydrolysates Affect the Structural and Mechanical Properties of Soy Protein-Wheat Gluten Extrudates Using High Moisture Extrusion
Authors: Ji Yan , Wang Zhaojun , Deng Qian , Chen Jie , He Zhiyong , Zeng Maomao , Qin Fang , Pan Hongyang
Published: February 2023

This study aimed to investigate the effect of hydrolyzed soy protein isolate (HSPI) as a plasticizer in the soy protein mixture-wheat gluten (SP-WG) extrudates on its structural and mechanical properties during high moisture extrusion. Those SP were prepared by mixing soy protein isolate (SPI) and HSPI with different ratios. HSPI primarily consisted of small molecular weight peptides measured with size exclusion chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The elastic modulus of SP-WG blends decreased with increased HSPI contents through the closed cavity rheometer. Adding HSPI at low concentrations (≤30 wt% of SP) enhanced a fibrous appearance and higher mechanical anisotropy while adding more HSPI resulted in a compact and brittle structure and tended to be isotropic. It can be concluded that the partial addition of HSPI as a plasticizer can promote the formation of a fibrous structure with enhanced mechanical anisotropy.
Control of Beany Flavor from Soybean Protein Raw Material in Plant-Based Meat Analog Processing
Authors: Yang Lingyu , Zhang Tianyu , Li He , Chen Tianpeng , Liu Xinqi
Published: February 2023

The development of plant-based meat analogs is currently hindered by the beany flavor generated by raw soybean protein and extrusion processing. Wide concern has led to extensive research on the generation and control of this unwanted flavor, as an understanding of its formation in raw protein and extrusion processing and methods through which to control its retention and release are of great significance for obtaining ideal flavor and maximizing food quality. This study examines the formation of beany flavor during extrusion processing as well as the influence of interaction between soybean protein and beany flavor compounds on the retention and release of the undesirable flavor. This paper discusses ways to maximize control over the formation of beany flavor during the drying and storage of raw materials and methods to reduce beany flavor in products by adjusting extrusion parameters. The degree of interaction between soybean protein and beany compounds was found to be dependent on conditions such as heat treatment and ultrasonic treatment. Finally, future research directions are proposed and prospected. This paper thus provides a reference for the control of beany flavor during the processing, storage, and extrusion of soybean raw materials used in the fast-growing plant-based meat analog industry.
Effectiveness of Dengue Awareness Calendar on Indigenous Population: Impact on Knowledge, Belief and Practice
Authors: Wong Li Ping , Rajandra Arulvani , Abd Jamil Juraina , AbuBakar Sazaly , Lin Yulan , Lee Hai Yen
Published: February 2023

Background: Dengue is prevalent among the indigenous community due to impoverished living conditions near the forest fringe areas and lack of health awareness. The study aims to determine the effect of a dengue awareness calendar on knowledge, belief, and practices (KBP) among the indigenous population. Method: A cross-sectional study was conducted in nine selected indigenous villages in Selangor, Malaysia. A dengue awareness calendar was distributed to the indigenous communities after pre-intervention. The KBP scores were compared between the pre-and post-intervention. Result: A total of 609 paired responses were obtained. Knowledge, perceived severity, cues to action, self-efficacy, and prevention practices were significantly improved after the intervention (p > 0.00). Participants with primary (Odds Ratio (OR) 2.627; 95% CI 1.338–5.160) and secondary level education (OR 2.263; 95% Cl 1.126–4.550) reported a high increment in practices score. High increments in dengue knowledge scores (OR 2.190; 95% CI 1.521–3.757, p < 0.00) were significantly more likely to report a high increment in practices score. Housewives (OR 0.535; 95% Cl 0.289–0.950), perceived severity (OR 0.349; 95% CI 0.184–0.662), and perceived susceptibility (OR 0.474; 95% CI 0.286–0.785) were significantly less likely to report an increment in prevention practices score. Conclusion: Findings inferred that the dengue awareness calendar significantly improved knowledge and practices. Our findings revealed the effectiveness of the dengue awareness calendar in dengue prevention among indigenous communities.
Clustering of Health and Oral Health-Compromising Behaviours in Army Personnel in Central Peninsular Malaysia
Authors: Azil Ahmad Asyraf , Yusof Zamros Yuzadi Mohd , Marhazlinda Jamaludin
Published: February 2023

Health- and oral health-compromising behaviours (HOHCBs) impact the health readiness of military personnel, resulting in decreased fitness performance, thus affecting combat readiness. This study aimed to identify the clustering patterns and number of HOHCBs in army personnel in Central Peninsular Malaysia. Thus, a cross-sectional study using a multistage sampling technique and a validated 42-item online questionnaire was conducted to assess ten health (medical screening, physical activity, sedentary lifestyle, smoking status, alcohol consumption, substance abuse, aggressive behaviours, sleep, and road safety habits) and five oral health behaviour domains (tooth brushing, fluoridated toothpaste use, flossing, dental visits, and bruxism). Each HOHCB was dichotomised into healthy and health-compromising behaviour and analysed using hierarchical agglomerative cluster analysis (HACA). With the majority being males (92.5%), of other ranks (96.8%), and healthy (83.9%), 2435 army members of a mean age of 30.3 years (SD = 5.9) participated, with a response rate of 100%. HACA identified two clustering patterns: (i) ‘high-risk behaviours’ (30 HOHCBs) and (ii) ‘most common risk behaviours’ (12 HOHCBs) with a mean clustering number of 14.1 (SD = 4.1). In conclusion, army personnel in Central Peninsular Malaysia displayed 2 broad HOHCB clustering patterns, ‘high-risk’ and ‘most common risk’, with an average of 14 HOHCB clusters per person.
Impact of Disability Status on Mortality in Patients with Gastric Cancer: A Nationwide Study Focusing on Regional Disparities
Authors: Lee Woo-Ri , Han Kyu-Tae , Choi Mingee , Park Seojin , Kim Woorim
Published: February 2023

Background: Disparities in mortality according to disability status require investment, as individuals with disabilities form the largest subset of the vulnerable population. This study aimed to investigate the association between mortality and disability status in patients with gastric cancer as well as how regional disparities modify this relationship. Methods: Data were obtained from the National Health Insurance claims database in South Korea for the period of 2006–2019. The outcome measures were all-cause 1-year, 5-year, and overall mortality. The main variable of interest was disability status, categorized into “no disability”, “mild disability”, and “severe disability”. A survival analysis based on the Cox proportional hazards model was conducted to analyze the association between mortality and disability status. Subgroup analysis was conducted according to region. Results: Of the 200,566 study participants, 19,297 (9.6%) had mild disabilities, and 3243 (1.6%) had severe disabilities. Patients with mild disabilities had higher 5- and overall mortality risks, and those with severe disabilities had higher 1-year, 5-year, and overall mortality risks than those without disabilities. These tendencies were generally maintained regardless of the region, but the magnitude of the differences in the mortality rates according to disability status was higher in the group residing in non-capital regions than in the group living in the capital city. Conclusion: Disability status was associated with all-cause mortality in patients with gastric cancer. The degree of the differences in mortality rates among those with “no disability”, “mild disability”, and “severe disability” was augmented in the group residing in non-capital regions.
Smoking Habits and Attitudes toward Smoking in Patients with Severe Mental Illness in Residential Facilities in Insular Greece
Authors: Botsari Ioanna , Marouli Georgia , Arvanitaki Aikaterini , Peritogiannis Vaios
Published: February 2023

Smoking may contribute to increased cardiovascular morbidity and mortality in patients with schizophrenia spectrum disorders. The objective of the present study is to explore the attitudes toward smoking in patients with severe mental illness in residential rehabilitation facilities in insular Greece. The patients (n = 103) were studied with the use of a questionnaire based on a semi-structured interview. Most of the participants (68.3%) were current regular smokers, had been smoking for 29 years and started smoking at an early age. The majority (64.8%) reported having tried to quit smoking in the past, and only half had been advised by a physician to quit. The patients agreed on the rules for smoking and believed that the staff should avoid smoking in the facility. The years of smoking were statistically significantly correlated to the educational level and the treatment with antidepressant medication. A statistical analysis showed that longer stay period in the facilities correlates with current smoking, an effort to quit and increased belief that smoking causes harm to health. Further research on the attitudes of patients in residential facilities toward smoking is needed, which could guide interventions for smoking cessation and should be assumed by all health professionals who are involved in the care of those patients.
Relationship between Vitreous IL-6 Levels, Gender Differences and C-Reactive Protein (CRP) in a Blood Sample of Posterior Uveitis
Authors: Sakai Atsushi , Tagami Mizuki , Katsuyama-Yoshikawa Atsuko , Misawa Norihiko , Haruna Yusuke , Azumi Atsushi , Honda Shigeru
Published: February 2023

This study retrospectively determined the relationship between vitreous IL-6 levels and clinical and laboratory data collected from uveitis patients. We examined an unknown cause of posterior uveitis, collecting vitreous fluid to investigate vitreous IL-6 levels. The samples were analyzed in consideration of clinical and laboratory factors, such as the male/female ratio. The present study included 82 eyes from 77 patients with a mean age of 66.20 ± 15.41 years. The IL-6 concentrations of the vitreous specimens were 6255.0 ± 14,108.3 pg/mL in males and 277.6 ± 746.3 pg/mL in females, which was found to be a statistically significant difference (p = 0.048) (n = 82). There was also a statistically significant correlation between vitreous IL-6 concentrations, serum C-reactive protein (CRP) value and white blood cell counts (WBCs) (n = 82). In multivariate analysis, vitreous IL-6 levels were significantly correlated with gender and CRP in all cases (p = 0.048 and p < 0.01, respectively) and were also significantly correlated with CRP in non-infectious uveitis (p < 0.01). In infectious uveitis, there were no significant differences between IL-6 level and several variables. Vitreous IL-6 concentrations were higher in males than in females in all cases. In non-infectious uveitis, vitreous IL-6 levels were correlated with serum CRP. These results might suggest that intraocular IL-6 levels depend on gender differences in posterior uveitis, and intraocular IL-6 levels in non-infectious uveitis may reflect systemic inflammations, including increased serum CRP.
Influence of Left Ventricular Diastolic Dysfunction on the Diagnostic Performance of Coronary Computed Tomography Angiography-Derived Fractional Flow Reserve
Authors: Xie Zhixin , Wu Tianlong , Mu Jing , Zhang Ping , Wang Xuan , Liang Tao , Weng Yihan , Luo Jianfang , Yu Huimin
Published: February 2023

Objectives: Our study aimed to demonstrate the influence of left ventricular (LV) diastolic dysfunction on the diagnostic performance of coronary computed tomography angiography-derived fractional flow reserve (CT-FFR). Methods: One hundred vessels from 90 patients were retrospectively analyzed. All patients underwent echocardiography, coronary computed tomography angiography (CCTA), CT-FFR, invasive coronary angiography (ICA), and fractional flow reserve (FFR). The study population was divided into normal and dysfunction groups according to the LV diastolic function, and the diagnostic performance in both groups was assessed. Results: There was a good correlation between CT-FFR and FFR (R = 0.768 p < 0.001) on a per-vessel basis. The sensitivity, specificity, and accuracy were 82.3%, 81.8%, and 82%, respectively. The sensitivity, specificity, and accuracy were 84.6%, 88.5%, and 87.2% in the normal group and 81%, 77.5%, and 78.7% in the dysfunction group, respectively. CT-FFR showed no statistically significant difference in the AUC in the normal group vs. the dysfunction group (AUC: 0.920 [95% CI 0.787–0.983] vs. 0.871 [95% CI 0.761–0.943], Z = 0.772 p = 0.440). However, there was still a good correlation between CT-FFR and FFR in the normal group (R = 0.767, p < 0.001) and dysfunction group (R = 0.767 p < 0.001). Conclusions: LV diastolic dysfunction had no effect on the diagnostic accuracy of CT-FFR. CT-FFR has good diagnostic performance in both LV diastolic dysfunction and the normal group and can be used as an effective tool for finding lesion-specific ischemia while screening for arterial disease in patients.
Relationship between Cholesterol-Related Lipids and Severe Acute Pancreatitis: From Bench to Bedside
Authors: Zhou Xiaoying , Jin Shengchun , Pan Jingyi , Lin Qingyi , Yang Shaopeng , Lu Yajing , Qiu Minhao , Ambe Peter C. , Basharat Zarrin , Zimmer Vincent , Wang Wei , Hong Wandong
Published: February 2023

It is well known that hypercholesterolemia in the body has pro-inflammatory effects through the formation of inflammasomes and augmentation of TLR (Toll-like receptor) signaling, which gives rise to cardiovascular disease and neurodegenerative diseases. However, the interaction between cholesterol-related lipids and acute pancreatitis (AP) has not yet been summarized before. This hinders the consensus on the existence and clinical importance of cholesterol-associated AP. This review focuses on the possible interaction between AP and cholesterol-related lipids, which include total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and apolipoprotein (Apo) A1, from the bench to the bedside. With a higher serum level of total cholesterol, LDL-C is associated with the severity of AP, while the persistent inflammation of AP is allied with a decrease in serum levels of cholesterol-related lipids. Therefore, an interaction between cholesterol-related lipids and AP is postulated. Cholesterol-related lipids should be recommended as risk factors and early predictors for measuring the severity of AP. Cholesterol-lowering drugs may play a role in the treatment and prevention of AP with hypercholesterolemia.
Concomitant Use of Elexacaftor/Tezacaftor/Ivacaftor and Etanercept in a Cystic Fibrosis Patient with Juvenile Idiopathic Arthritis
Authors: Orsini Sara Immacolata , Marrani Edoardo , Pagnini Ilaria , Taccetti Giovanni , Terlizzi Vito , Simonini Gabriele
Published: February 2023

Patients with cystic fibrosis often complain of joint manifestations. However, only a few studies have reported the association between cystic fibrosis and juvenile idiopathic arthritis and addressed the therapeutic challenges of these patients. We describe the first paediatric case of a patient affected by cystic fibrosis, Basedow’s disease and juvenile idiopathic arthritis who was contemporarily treated with elexacaftor/tezacaftor/ivacaftor (ELX/TEZ/IVA) and anti-tumor necrosis factor α (anti-TNFα). This report seems to reassure regarding the potential side effects of these associations. Moreover, our experience suggests that anti-TNFα is an effective option in CF patients affected by juvenile idiopathic arthritis, and is even safe for children receiving a triple CFTR modulator.
The Patient’s Physiological Status at the Start Determines the Success of the Inpatient Cardiovascular Rehabilitation Program
Authors: Odrovicsné-Tóth Anna , Thauerer Bettina , Stritzinger Barbara , Kullich Werner , Salzer Andreas , Skoumal Martin , Steinecker-Frohnwieser Bibiane
Published: February 2023

Multidisciplinary inpatient rehabilitation plays an important role in the recovery of patients with cardiovascular diseases (CVDs). Lifestyle changes, achieved by exercise, diet, weight loss and patient education programs, are the first steps to a healthier life. Advanced glycation end products (AGEs) and their receptor (RAGE) are known to be involved in CVDs. Clarification on whether initial AGE levels can influence the rehabilitation outcome is important. Serum samples were collected at the beginning and end of the inpatient rehabilitation stay and analyzed for parameters: lipid metabolism, glucose status, oxidative stress, inflammation and AGE/RAGE-axis. As result, a 5% increase in the soluble isoform RAGE (sRAGE) (T0: 891.82 ± 44.97 pg/mL, T1: 937.17 ± 43.29 pg/mL) accompanied by a 7% decrease in AGEs (T0: 10.93 ± 0.65 µg/mL, T1: 10.21 ± 0.61 µg/mL) was shown. Depending on the initial AGE level, a significant reduction of 12.2% of the AGE activity (quotient AGE/sRAGE) was observed. We found that almost all measured factors improved. Summarizing, CVD-specific multidisciplinary rehabilitation positively influences disease-associated parameters, and thus provides an optimal starting point for subsequent disease-modifying lifestyle changes. Considering our observations, the initial physiological situations of patients at the beginning of their rehabilitation stay seem to play a decisive role regarding the assessment of rehabilitation success.
Effect of Preoperative Administration of Intravenous Ferric Carboxymaltose in Patients with Iron Deficiency Anemia after Off-Pump Coronary Artery Bypass Grafting: A Randomized Controlled Trial
Authors: Kim Hyo-Hyun , Park Eun Hye , Lee Seung Hyun , Yoo Kyung-Jong , Youn Young-Nam
Published: February 2023

Patients scheduled for cardiac surgery often have anemia and iron deficiency. We investigated the effect of the preoperative administration of intravenous ferric carboxymaltose (IVFC) in patients with iron deficiency anemia (IDA) who were due to undergo off-pump coronary artery bypass grafting (OPCAB). Patients who were due to undergo elective OPCAB between February 2019 and March 2022 who had IDA (n = 86) were included in this single center, randomized, parallel-group controlled study. The participants were randomly assigned (1:1) to receive either IVFC or placebo treatment. Postoperative hematologic parameters [hemoglobin (Hb), hematocrit, serum iron concentration, total iron-binding capacity, transferrin saturation, transferrin concentration, and ferritin concentration] and the changes in these parameters during the follow-up period were the primary and secondary outcomes, respectively. The tertiary endpoints were early clinical outcomes, such as the volume of mediastinal drainage and the need for blood transfusions. IVFC treatment significantly reduced the need for red blood cell (RBC) and platelet transfusions. Despite receiving fewer RBC transfusions, patients in the treatment group had higher levels of Hb, hematocrit, and serum iron and ferritin concentrations during weeks 1 and 12 after surgery. No serious adverse events occurred during the study period. Preoperative IVFC treatment in patients with IDA undergoing OPCAB improved the values of the hematologic parameters and iron bioavailability. Therefore, is a useful strategy for stabilizing patients prior to OPCAB.
Assessing the Outcomes of Patients with Severe SARS-CoV-2 Infection after Therapeutic Plasma Exchange by Number of TPE Sessions
Authors: Porosnicu Tamara Mirela , Sandesc Dorel , Jipa Daniel , Gindac Ciprian , Oancea Cristian , Bratosin Felix , Fericean Roxana Manuela , Kodimala Shiva Charana , Pilut Ciprian Nicolae , Nussbaum Laura Alexandra , Sirbu Ioan Ovidiu
Published: February 2023

The high mortality risk in severe SARS-CoV-2 infections is tightly correlated to the extreme elevation of inflammatory markers. This acute accumulation of inflammatory proteins can be cleared using plasma exchange (TPE), commonly known as plasmapheresis, although the available data on performing TPE in COVID-19 patients is limited regarding the optimal treatment protocol. The purpose for this study was to examine the efficacy and outcomes of TPE based on different treatment methods. A thorough database search was performed to identify patients from the Intensive Care Unit (ICU) of the Clinical Hospital of Infectious Diseases and Pneumology between March 2020 and March 2022 with severe COVID-19 that underwent at least one session of TPE. A total of 65 patients satisfied the inclusion criteria and were eligible for TPE as a last resort therapy. Of these, 41 patients received 1 TPE session, 13 received 2 TPE sessions, and the remaining 11 received more than 2 TPE sessions. It was observed that IL-6, CRP, and ESR decreased significantly after all sessions were performed in all three groups, with the highest decrease of IL-6 in those who received >2 TPE sessions (from 305.5 pg/mL to 156.0 pg/mL). Interestingly, there was a significant increase in leucocyte levels after TPE, but there was no significant difference in MAP changes, SOFA score, APACHE 2 score, or the PaO2/FiO2 ratio. The ROX index was significantly higher among the patients who underwent more than two TPE sessions, with an average of 11.4, compared to 6.5 in group 1 and 7.4 in group 2, which increased significantly after TPE. Nevertheless, the mortality rate was very high (72.3%), and the Kaplan–Meier analysis identified no significant difference in survival according to the number of TPE sessions. TPE can be used as last resort salvage therapy that can be regarded as an alternative treatment method when the standard management of these patients fails. It significantly decreases the inflammatory status measured via IL-6, CRP, and WBC, as well as demonstrating an improvement of the clinical status measured via PaO2/FiO2, and duration of hospitalization. However, the survival rate does not seem to change with the number of TPE sessions. Based on the survival analysis, one session of TPE as last resort treatment in patients with severe COVID-19 proved to have the same effect as repeated TPE sessions of 2 or more.
Belief Inflexibility and Cognitive Biases in Anorexia Nervosa—The Role of the Bias against Disconfirmatory Evidence and Its Clinical and Neuropsychological Correlates
Authors: Tenconi Elena , Meregalli Valentina , Buffa Adriana , Collantoni Enrico , Cavallaro Roberto , Meneguzzo Paolo , Favaro Angela
Published: February 2023

The aim of this study was to explore, in a sample of patients with a diagnosis of AN, the ability to question their first impression and, in particular, the willingness to integrate their prior ideas and thoughts with additional progressive incoming information. A total of 45 healthy women and 103 patients with a diagnosis of AN, consecutively admitted to the Eating Disorder Padova Hospital–University Unit, underwent a broad clinical and neuropsychological assessment. All participants were administered the Bias Against Disconfirmatory Evidence (BADE) task, which specifically investigates belief integration cognitive bias. Acute AN patients showed a significantly greater bias toward disconfirming their previous judgment, in comparison to healthy women (BADE score, respectively, 2.5 ± 2.0 vs. 3.3 ± 1.6; Mann–Whitney test, p = 0.012). A binge-eating/purging subtype of AN individuals, compared to restrictive AN patients and controls, showed greater disconfirmatory bias and also a significant propensity to uncritically accept implausible interpretations (BADE score, respectively, 1.55 ± 1.6 and 2.70 ± 1.97 vs. 3.33 ± 1.63; Kruskal–Wallis test, p = 0.002 and liberal acceptance score, respectively, 1.32 ± 0.93 and 0.92 ± 1.21 vs. 0.98 ± 0.75; Kruskal–Wallis test p = 0.03). Abstract thinking skills and cognitive flexibility, as well as high central coherence, are neuropsychological aspects positively correlated with cognitive bias, in both patients and controls. Research into belief integration bias in AN population could enable us to shed light on hidden dimensional aspects, facilitating a better understanding of the psychopathology of a disorder that is so complex and difficult to treat.
“Neuro-Fiber Mapping”: An Original Concept of Spinal Cord Neural Network Spatial Targeting Using Live Electrostimulation Mapping to (Re-)Explore the Conus Medullaris Anatomy
Authors: Rigoard Philippe , Moens Maarten , Goudman Lisa , Le Tutour Tom , Rochette Michel , Dany Jonathan , Et Talby Mohamed , Roulaud Manuel , Hervochon Rémi , Ounajim Amine , Nivole Kévin , David Romain , Billot Maxime
Published: February 2023

Spinal cord (SC) anatomy is often assimilated to a morphologically encapsulated neural entity, but its functional anatomy remains only partially understood. We hypothesized that it could be possible to re-explore SC neural networks by performing live electrostimulation mapping, based on “super-selective” spinal cord stimulation (SCS), originally designed as a therapeutical tool to address chronic refractory pain. As a starting point, we initiated a systematic SCS lead programming approach using live electrostimulation mapping on a chronic refractory perineal pain patient, previously implanted with multicolumn SCS at the level of the conus medullaris (T12-L1). It appeared possible to (re-)explore the classical anatomy of the conus medullaris using statistical correlations of paresthesia coverage mappings, resulting from 165 different electrical configurations tested. We highlighted that sacral dermatomes were not only located more medially but also deeper than lumbar dermatomes at the level of the conus medullaris, in contrast with classical anatomical descriptions of SC somatotopical organization. As we were finally able to find a morphofunctional description of “Philippe–Gombault’s triangle” in 19th-century historical textbooks of neuroanatomy, remarkably matching these conclusions, the concept of “neuro-fiber mapping” was introduced.
Perioperative Drug Treatment in Pancreatic Surgery—A Systematic Review and Meta-Analysis
Authors: Rompen Ingmar F. , Merz Daniela C. , Alhalabi Karam T. , Klotz Rosa , Kalkum Eva , Pausch Thomas M. , Strothmann Hendrik , Probst Pascal
Published: February 2023

Introduction: Pancreatic resections for malignant or benign diseases are associated with major morbidity and changes in physiology. To reduce perioperative complications and enhance recovery, many types of perioperative medical management have been introduced. The aim of this study was to provide an evidence-based overview on the best perioperative drug treatment. Methods: The electronic bibliographic databases Medline, Embase, CENTRAL, and Web of Science were systematically searched for randomized controlled trials (RCT) evaluating perioperative drug treatments in pancreatic surgery. The investigated drugs were somatostatin analogues, steroids, pancreatic enzyme replacement therapy (PERT), prokinetic therapy, antidiabetic drugs, and proton pump inhibitors (PPI). Targeted outcomes in each drug category were meta-analyzed. Results: A total of 49 RCT were included. The analysis of somatostatin analogues showed a significantly lower incidence of postoperative pancreatic fistula (POPF) in the somatostatin group compared to the control group (OR 0.58, 95% CI: 0.45 to 0.74). The comparison of glucocorticoids versus placebo showed significantly less POPF in the glucocorticoid group (OR 0.22, 95% CI: 0.07 to 0.77). There was no significant difference in DGE when erythromycin was compared to placebo (OR 0.33, 95% CI: 0.08 to 1.30). The other investigated drug regimens could only be analyzed qualitatively. Conclusion: This systematic review provides a comprehensive overview on perioperative drug treatment in pancreatic surgery. Some often-prescribed perioperative drug treatments lack high quality evidence and further research is needed.
Fisheries Biology and Basic Life-Cycle Characteristics of the Invasive Blue Crab Callinectes sapidus Rathbun in the Estuarine Area of the Evros River (Northeast Aegean Sea, Eastern Mediterranean)
Authors: Kevrekidis Kosmas , Kevrekidis Theodoros , Mogias Athanasios , Boubonari Theodora , Kantaridou Foteini , Kaisari Nikoletta , Malea Paraskevi , Dounas Costas , Thessalou-Legaki Maria
Published: February 2023

The catch per unit effort (CPUE), population structure, sex ratio, and reproductive aspects of the invasive blue crab Callinectes sapidus Rathbun were studied in Monolimni Lagoon and the adjacent coastal waters in the estuarine area of the Evros River (Northeast Aegean Sea, Eastern Mediterranean). The CPUE varied both seasonally and spatially; higher values were recorded in summer and fall in the lagoonal stations characterized by shallow depth (<0.5 m) and a dense meadow of the angiosperm Ruppia maritima, whereas significantly lower CPUE was recorded in the adjacent coastal area. The bottom temperature was positively correlated with CPUE. Μodal progression analysis estimated a three-modal size–frequency distribution for both sexes corresponding to the 0+, 1+, and 2+ age classes. Females attained a significantly larger size (carapace width (CW)) than males. Crab size decreased from the marine area to the lagoonal stations. The sex ratio was estimated at 2.45:1 (♂/♀). Males dominated in all months, except for October, when an equal sex ratio was observed. The mean size at maturity (L50) of females was estimated at 124 mm CW. Females with mature ovaries were found from February to October. Ovigerous females were observed from May to October in the coastal area, where a spawning peak occurred in September. In the Evros River estuary, the American blue crab exhibits a life cycle that seems to conform to the general complex life-cycle pattern of the species along the mid-Atlantic coast.
Underwater Optical Image Restoration Method for Natural/Artificial Light
Authors: Zhang Tianchi , Li Qian , Li Yusong , Liu Xing
Published: February 2023

This paper investigates the underwater optical image restoration method under the background of underwater target detection based on optical vision in AUVs. The light source used for AUV detection is different when the AUV operates in different depths. The natural light source is used in shallow water and the artificial light source is used in deep water. This paper investigates underwater optical image restoration in these two light conditions. Aiming at the problem of image blurring in underwater optical images, the traditional underwater image restoration method based on scattering model can obtain satisfactory image restoration performance in natural light conditions. However, it cannot obtain the same image restoration result in artificial light conditions. To solve this problem, this paper presents an improved underwater optical image restoration method based on the scattering model. The scattering model and power spectrum are used to solve the initial parameters of the filter, and the parameters are optimized based on an evaluation index. The index of image definition is introduced to evaluate the restoration performance and to achieve the satisfactory image restoration result in both natural light and artificial light conditions. The effectiveness of the presented method is verified by experiments.
Syrian SMEs in Times of COVID-19 Pandemic: Challenges, Adaptation, and Policy Measures
Authors: Akkad Bana Abdulmajid , Mouselli Sulaiman
Published: February 2023

SMEs constitute the backbone of the Syrian economy and have suffered manifold challenges due to the continuous Syrian war. COVID-19 added further pressures on Syrian SMEs and forced them to take certain adaptation strategies to survive. This paper aims to investigate the main challenges that face Syrian SMEs during the pandemic and illustrate how they respond to adversities that emerged from governmental intervention to control the spread of the virus. It also discusses the measures initiated by the government to support SMEs during the pandemic. Through interviewing persons from the Syrian SMEs’ ecosystem, we find that high interest rates on SMEs’ loans decline on demand as well as high inflation represent the main challenges. SMEs respond to these challenges by marketing products online, stock procurement, and strengthening connections with stakeholders. We recommend the Syrian authorities reduce lending rates and increase loan sizes available to SMEs to help them overcome the pandemic adversities. Innovative sources of funding, such as venture capital and equity partnerships, could reduce the funding costs of SMEs. Moreover, SMEs will immensely benefit from training in digital tools to enhance their expansion and survival opportunities. Furthermore, bazaars should be organized during the year to give SMEs the opportunity to gain continuous access to markets. In addition, incubation services should be revised, particularly to SMEs with great potential to grow, to create the suitable environment for them to scale and flourish.