bluuebunny/crossref_metadata_2025 huggingface dataset:
Biological Activity of Horehound (Marrubium vulgare L.) Herb Grown in Poland and Its Phytochemical Composition
Authors: Michalak Monika , Stryjecka Małgorzata , Zagórska-Dziok Martyna , Żarnowiec Paulina
Published: June 2024

Marrubium vulgare (Lamiaceae) is a plant which has long been known and used in traditional medicine for various purposes. However, few recent studies have documented its chemical composition and biological properties. The present study investigated the phytochemical composition of horehound, as well as its protective, antioxidant, and antimicrobial potential. GC-MS analysis revealed that the major components of horehound essential oil are E-caryophyllene (35.7%), germacrene D (25.2%), and bicyclogermacrene (10.6%). The biological activity of horehound hydroethanolic herb extract derives from multiple chemical compounds, including polyphenols (55.72 mg/mL), flavonoids (11.01 mg/mL), phenolic acids (4.33 mg/mL), and tannins (4.46 mg/mL). Chromatographic analyses of the extract identified 12 phenolic compounds, of which ferulic acid, catechin, quercetin, protocatechuic acid, rutin, and syringic acid (35.42, 24.69, 20.65, 18.70, 14.46, and 12.69 mg/mL, respectively) were the main constituents. Its DPPH radical scavenging ability was 68.29%, while its antioxidant properties, determined by the FRAP method, were at the level of 1.22 mmol/L. Moreover, M. vulgare extract decreased the level of intracellular reactive oxygen species in the fibroblasts and keratinocytes in vitro, achieving the strongest antioxidant effect at a concentration of 2.5% in the case of both types of skin cells. Extracts from the horehound herb showed significant antimicrobial and anti-biofilm activity, confirming the plant’s potential in therapeutic applications against various microbial pathogens (gram-positive and gram-negative bacteria and fungi). The research results demonstrate the protective effect of horehound extract on the viability of both fibroblasts and keratinocytes in vitro. To sum up, M. vulgare, as a valuable natural material with high preventive and therapeutic effectiveness, is a potential candidate for new applications in the pharmaceutical and cosmetics industries.
Exploring the Role of Ubiquitin-Proteasome System in the Pathogenesis of Parkinson’s Disease
Authors: Zhao Yiting , Lin Man , Zhai Fengguang , Chen Jun , Jin Xiaofeng
Published: June 2024

Parkinson’s disease (PD) is a prevalent neurodegenerative disorder among the elderly population. The pathogenesis of PD encompasses genetic alterations, environmental factors, and age-related neurodegenerative processes. Numerous studies have demonstrated that aberrant functioning of the ubiquitin–proteasome system (UPS) plays a crucial role in the initiation and progression of PD. Notably, E3 ubiquitin ligases serve as pivotal components determining substrate specificity within UPS and are intimately associated with the regulation of various proteins implicated in PD pathology. This review comprehensively summarizes the mechanisms by which E3 ubiquitin ligases and deubiquitinating enzymes modulate PD-associated proteins and signaling pathways, while exploring the intricate relationship between UPS dysfunctions and PD etiology. Furthermore, this article discusses recent research advancements regarding inhibitors targeting PD-related E3 ubiquitin ligases.
Evaluating the Antihyperalgesic Potential of Sildenafil–Metformin Combination and Its Impact on Biochemical Markers in Alloxan-Induced Diabetic Neuropathy in Rats
Authors: Pușcașu Ciprian , Negreș Simona , Zbârcea Cristina Elena , Ungurianu Anca , Ștefănescu Emil , Blebea Nicoleta Mirela , Chiriță Cornel
Published: June 2024

(1) Background: Globally, about 600 million people are afflicted with diabetes, and one of its most prevalent complications is neuropathy, a debilitating condition. At the present time, the exploration of novel therapies for alleviating diabetic-neuropathy-associated pain is genuinely captivating, considering that current therapeutic options are characterized by poor efficacy and significant risk of side effects. In the current research, we evaluated the antihyperalgesic effect the sildenafil (phosphodiesterase-5 inhibitor)–metformin (antihyperglycemic agent) combination and its impact on biochemical markers in alloxan-induced diabetic neuropathy in rats. (2) Methods: This study involved a cohort of 70 diabetic rats and 10 non-diabetic rats. Diabetic neuropathy was induced by a single dose of 130 mg/kg alloxan. The rats were submitted to thermal stimulus test using a hot–cold plate and to tactile stimulus test using von Frey filaments. Moreover, at the end of the experiment, the animals were sacrificed and their brains and livers were collected to investigate the impact of this combination on TNF-α, IL-6, nitrites and thiols levels. (3) Results: The results demonstrated that all sildenafil–metformin combinations decreased the pain sensitivity in the von Frey test, hot plate test and cold plate test. Furthermore, alterations in nitrites and thiols concentrations and pro-inflammatory cytokines (specifically TNF-α and IL-6) were noted following a 15-day regimen of various sildenafil–metformin combinations. (4) Conclusions: The combination of sildenafil and metformin has a synergistic effect on alleviating pain in alloxan-induced diabetic neuropathy rats. Additionally, the combination effectively decreased inflammation, inhibited the rise in NOS activity, and provided protection against glutathione depletion.
Synthesis of Gd-DTPA Carborane-Containing Compound and Its Immobilization on Iron Oxide Nanoparticles for Potential Application in Neutron Capture Therapy
Authors: Korolkov Ilya V. , Zaboronok Alexander , Izbasar Kairat A. , Bekbol Zhangali A. , Lissovskaya Lana I. , Zibert Alexandr V. , Shakirzyanov Rafael I. , Korganbayeva Luiza N. , Yang Haolan , Ishikawa Eiichi , Zdorovets Maxim V.
Published: June 2024

Cancer is one of the leading causes of global mortality, and its incidence is increasing annually. Neutron capture therapy (NCT) is a unique anticancer modality capable of selectively eliminating tumor cells within normal tissues. The development of accelerator-based, clinically mountable neutron sources has stimulated a worldwide search for new, more effective compounds for NCT. We synthesized magnetic iron oxide nanoparticles (NPs) that concurrently incorporate boron and gadolinium, potentially enhancing the effectiveness of NCT. These magnetic nanoparticles underwent sequential modifications through silane polycondensation and allylamine graft polymerization, enabling the creation of functional amino groups on their surface. Characterization was performed using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), energy dispersive X-ray (EDX), dynamic light scattering (DLS), thermal gravimetric analysis (TGA), and transmission electron microscopy (TEM). ICP-AES measurements indicated that boron (B) content in the NPs reached 3.56 ppm/mg, while gadolinium (Gd) averaged 0.26 ppm/mg. Gadolinium desorption was observed within 4 h, with a peak rate of 61.74%. The biocompatibility of the NPs was confirmed through their relatively low cytotoxicity and sufficient cellular tolerability. Using NPs at non-toxic concentrations, we obtained B accumulation of up to 5.724 × 1010 atoms per cell, sufficient for successful NCT. Although limited by its content in the NP composition, the Gd amount may also contribute to NCT along with its diagnostic properties. Further development of the NPs is ongoing, focusing on increasing the boron and gadolinium content and creating active tumor targeting.
Influence of Mechanical Loading on the Process of Tribochemical Action on Physicochemical and Biopharmaceutical Properties of Substances, Using Lacosamide as an Example: From Micronisation to Mechanical Activation
Authors: Uspenskaya Elena V. , Kuzmina Ekaterina , Quynh Hoang Thi Ngoc , Komkova Maria A. , Kazimova Ilaha V. , Timofeev Aleksey A.
Published: June 2024

Many physical and chemical properties of solids, such as strength, plasticity, dispersibility, solubility and dissolution are determined by defects in the crystal structure. The aim of this work is to study in situ dynamic, dispersion, chemical, biological and surface properties of lacosamide powder after a complete cycle of mechanical loading by laser scattering, electron microscopy, FR-IR and biopharmaceutical approaches. The SLS method demonstrated the spontaneous tendency toward surface-energy reduction due to aggregation during micronisation. DLS analysis showed conformational changes of colloidal particles as supramolecular complexes depending on the loading time on the solid. SEM analysis demonstrated the conglomeration of needle-like lacosamide particles after 60 min of milling time and the transition to a glassy state with isotropy of properties by the end of the tribochemistry cycle. The following dynamic properties of lacosamide were established: elastic and plastic deformation boundaries, region of inhomogeneous deformation and fracture point. The ratio of dissolution-rate constants in water of samples before and after a full cycle of loading was 2.4. The lacosamide sample, which underwent a full cycle of mechanical loading, showed improved kinetics of API release via analysis of dissolution profiles in 0.1 M HCl medium. The observed activation-energy values of the cell-death biosensor process in aqueous solutions of the lacosamide samples before and after the complete tribochemical cycle were 207 kJmol−1 and 145 kJmol−1, respectively. The equilibrium time of dissolution and activation of cell-biosensor death corresponding to 20 min of mechanical loading on a solid was determined. The current study may have important practical significance for the transformation and management of the properties of drug substances in solid form and in solutions and for increasing the strength of drug matrices by pre-strain hardening via structural rearrangements during mechanical loading.
In Vitro Metabolism and Transport Characteristics of Zastaprazan
Authors: Lee Min Seo , Lee Jihoon , Pang Minyoung , Kim John , Cha Hyunju , Cheon Banyoon , Choi Min-Koo , Song Im-Sook , Lee Hye Suk
Published: June 2024

Zastaprazan (JP-1366), a novel potassium-competitive acid blocker, is a new drug for the treatment of erosive esophagitis. JP-1366 is highly metabolized in human, mouse, and dog hepatocytes but moderately metabolized in rat and monkey hepatocytes when estimated from the metabolic stability of this compound in hepatocyte suspension and when 18 phase I metabolites and 5 phase II metabolites [i.e., N-dearylation (M6), hydroxylation (M1, M19, M21), dihydroxylation (M7, M8, M14, M22), trihydroxylation (M13, M18), hydroxylation and reduction (M20), dihydroxylation and reduction (M9, M16), hydrolysis (M23), hydroxylation and glucuronidation (M11, M15), hydroxylation and sulfation (M17), dihydroxylation and sulfation (M10, M12), N-dearylation and hydroxylation (M3, M4), N-dearylation and dihydroxylation (M5), and N-dearylation and trihydroxylation (M2)] were identified from JP-1366 incubation with the hepatocytes from humans, mice, rats, dogs, and monkeys. Based on the cytochrome P450 (CYP) screening test and immune-inhibition analysis with CYP antibodies, CYP3A4 and CYP3A5 played major roles in the metabolism of JP-1366 to M1, M3, M4, M6, M8, M9, M13, M14, M16, M18, M19, M21, and M22. CYP1A2, 2C8, 2C9, 2C19, and 2D6 played minor roles in the metabolism of JP-1366. UDP-glucuronosyltransferase (UGT) 2B7 and UGT2B17 were responsible for the glucuronidation of M1 to M15. However, JP-1366 and active metabolite M1 were not substrates for drug transporters such as organic cation transporter (OCT) 1/2, organic anion transporter (OAT) 1/3, organic anion transporting polypeptide (OATP)1B1/1B3, multidrug and toxic compound extrusion (MATE)1/2K, P-glycoprotein (P-gp), and breast cancer-resistant protein (BCRP). Only M1 showed substrate specificity for P-gp. The findings indicated that drug-metabolizing enzymes, particularly CYP3A4/3A5, may have a significant role in determining the pharmacokinetics of zastaprazan while drug transporters may only have a small impact on the absorption, distribution, and excretion of this compound.
Liposome Encapsulation Enhances the Antidiabetic Efficacy of Silibinin
Authors: Dinić Svetlana , Arambašić Jovanović Jelena , Uskoković Aleksandra , Jovanović Aleksandra , Grdović Nevena , Rajić Jovana , Đorđević Marija , Sarić Ana , Bugarski Branko , Vidaković Melita , Mihailović Mirjana
Published: June 2024

Silibinin has considerable therapeutic potential for the treatment of diabetes through anti-inflammatory, antioxidant, and immunomodulatory properties. However, the therapeutic application of silibinin is quite limited due to its poor bioavailability. In the present study, an attempt was made to improve the antidiabetic efficacy of silibinin by its encapsulation in liposomal vesicles. The liposomes with a high encapsulation efficiency of silibinin (96%) and a zeta potential of −26.2 ± 0.6 mV were developed and studied using nicotinamide/streptozotocin-induced diabetic rats. Administration of silibinin-loaded liposomes to diabetic rats lowered glucose levels, increased insulin levels, and improved pancreatic islet architecture. The anti-inflammatory effect of silibinin-loaded liposomes was demonstrated by a decrease in serum C-reactive protein (CRP) levels and a reduced deposition of collagen fibers in the islets of diabetic rats. Furthermore, silibinin-loaded liposomes were more efficient in lowering glucose, alanine transaminase, triglyceride, and creatinine levels in diabetic rats than pure silibinin. In addition, silibinin-loaded liposomes had a significantly better effect on beta-cell mass and Glut2 glucose receptor distribution in diabetic islets than pure silibinin. The present results clearly show that liposome encapsulation of silibinin enhances its antidiabetic efficacy, which may contribute to the therapeutic benefit of silibinin in the treatment of diabetes and its complications.
In Vitro and In Vivo Evaluation of Lactoferrin-Modified Liposomal Etomidate with Enhanced Brain-Targeting Effect for General Anesthesia
Authors: Wu Ailing , Shi Houyin , Yang Luhan , Zhang Hao , Nan Xichen , Zhang Dan , Zhang Zhuo , Zhang Chun , Chen Siwei , Fu Xiujuan , Ou Lilan , Wang Lulu , Shi Yanyan , Liu Hao
Published: June 2024

Etomidate is a general anesthetic that has shown good hemodynamic stability without significant cardiovascular or respiratory depression. Despite several kinds of dosage forms having been reported for this drug, formulation types are very limited in clinical practice, and brain-targeted formulations for this central nervous system (CNS) drug have been rarely reported. Moreover, studies on the biocompatibility, toxicity, and anesthetic effects of the etomidate preparations in vivo were inadequate. The present study was to develop lactoferrin-modified liposomal etomidate (Eto-lip-LF) for enhanced drug distribution in the brain and improved anesthetic effects. Eto-lip-LF had good stability for storage and hemocompatibility for intravenous injection. Compared with the non-lactoferrin-containing liposomes, the lactoferrin-modified liposomes had notably enhanced brain-targeting ability in vivo, which was probably realized by the binding of transferrin with the transferrin and lactoferrin receptors highly distributed in the brain. Eto-lip-LF had a therapeutic index of about 25.3, higher than that of many other general anesthetics. Moreover, compared with the commercial etomidate emulsion, Eto-lip-LF could better achieve rapid onset of general anesthesia and rapid recovery from anesthesia, probably due to the enhanced drug delivery to the brain. The above results demonstrated the potential of this lactoferrin-modified liposomal etomidate to become an alternative preparation for clinical general anesthesia.
Lipid Biomimetic Models as Simple Yet Complex Tools to Predict Skin Permeation and Drug–Membrane Biophysical Interactions
Authors: Fernandes Eduarda , Lopes Carla M. , Lúcio Marlene
Published: June 2024

The barrier function of the skin is primarily determined by its outermost layer, the Stratum Corneum (SC). The SC consists of corneocytes embedded in a lipid matrix composed mainly of ceramides, cholesterol, and free fatty acids in equimolar proportions and is organised in a complex lamellar structure with different periodicities and lateral packings. This matrix provides a diffusion pathway across the SC for bioactive compounds that are administered to the skin. In this regard, and as the skin administration route has grown in popularity, there has been an increase in the use of lipid mixtures that closely resemble the SC lipid matrix, either for a deeper biophysical understanding or for pharmaceutical and cosmetic purposes. This review focuses on a systematic analysis of the main outcomes of using lipid mixtures as SC lipid matrix models for pharmaceutical and cosmetic purposes. Thus, a methodical evaluation of the main outcomes based on the SC structure is performed, as well as the main recent developments in finding suitable new in vitro tools for permeation testing based on lipid models.
Transcriptome Analysis of the Regulatory Mechanisms of Holly (Ilex dabieshanensis) under Salt Stress Conditions
Authors: Chen Hong , Li Huihui , Chong Xinran , Zhou Ting , Lu Xiaoqing , Wang Xiaolong , Zheng Bingsong
Published: June 2024

The holly Ilex dabieshanensis K. Yao & M. B. Deng, a tree endemic to the Dabieshan Mountains region in China, is a commonly used landscaping plant. Like other crops, its growth is affected by salt stress. The molecular mechanism underlying salt tolerance in holly is still unclear. In this study, we used NaCl treatment and RNA sequencing (RNA-seq) at different times to identify the salt stress response genes of holly. A total of 4775 differentially expressed genes (DEGs) were identified. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of the DEGs obtained at different salt treatment times (3, 6, 9, 12, and 24 h), as compared to control (ck, 0 h), showed that plant hormone signal transduction and carotenoid biosynthesis were highly enriched. The mechanism by which holly responds to salt stress involves many plant hormones, among which the accumulation of abscisic acid (ABA) and its signal transduction may play an important role. In addition, ion homeostasis, osmotic metabolism, accumulation of antioxidant enzymes and nonenzymatic antioxidant compounds, and transcription factors jointly regulate the physiological balance in holly, providing important guarantees for its growth and development under conditions of salt stress. These results lay the foundation for studying the molecular mechanisms of salt tolerance in holly and for the selection of salt-tolerant varieties.
Phosphorus/Bromine Synergism Improved the Flame Retardancy of Polyethylene Terephthalate Foams
Authors: Du Jia , Zheng Jiaxin , Xin Chunling , He Yadong
Published: June 2024

Polyethylene terephthalate (PET) foams have the characteristics of being lightweight and high strength, as well as offering good heat resistance, minimal water absorption, etc., and they have been widely used in the wind power field. In addition, they are being promisingly applied in automotive, rail, marine, construction, and other related fields. Therefore, the flame retardancy(FR) of PET foams is an issue that requires investigation. The addition of flame retardants would affect the chain extension reaction, viscoelasticity, and foamability of PET. In this study, zinc diethyl hypophosphite (ZDP) and decabromodiphenylethane (DBDPE) were used to form a synergistic FR system, in which ZDP is an acid source and DBDPE is a gas source, and both of them synergistically produced an expanded carbon layer to improve the flame retardancy of PET foams. The ratio of ZDP and DBDPE is crucial for the carbon yield and the expansion and thermal stability of the char layers. At the ZDP/DBDPE ratios of 9/3 and 7/5, the thickness of the char layers is about 3–4 mm, the limiting oxygen index (LOI) values of FR modified PET are 32.7% and 33.6%, respectively, and the vertical combustion tests both reached the V-0 level. As for the extruded phosphorous/bromine synergism FR PET foams, ZDP/DBDPE ratios of 3:1 and 2:1 were applied. As a result, the vertical combustion grade of foamed specimens could still reach V-0 grade, and the LOI values are all over 27%, reaching the refractory grade.
Theoretical Analysis of Light-Actuated Self-Sliding Mass on a Circular Track Facilitated by a Liquid Crystal Elastomer Fiber
Authors: Wei Lu , Hu Junjie , Wang Jiale , Wu Haiyang , Li Kai
Published: June 2024

Self-vibrating systems obtaining energy from their surroundings to sustain motion can offer great potential in micro-robots, biomedicine, radar systems, and amusement equipment owing to their adaptability, efficiency, and sustainability. However, there is a growing need for simpler, faster-responding, and easier-to-control systems. In the study, we theoretically present an advanced light-actuated liquid crystal elastomer (LCE) fiber–mass system which can initiate self-sliding motion along a rigid circular track under constant light exposure. Based on an LCE dynamic model and the theorem of angular momentum, the equations for dynamic control of the system are deduced to investigate the dynamic behavior of self-sliding. Numerical analyses show that the theoretical LCE fiber–mass system operates in two distinct states: a static state and a self-sliding state. The impact of various dimensionless variables on the self-sliding amplitude and frequency is further investigated, specifically considering variables like light intensity, initial tangential velocity, the angle of the non-illuminated zone, and the inherent properties of the LCE material. For every increment of π/180 in the amplitude, the elastic coefficient increases by 0.25% and the angle of the non-illuminated zone by 1.63%, while the light intensity contributes to a 20.88% increase. Our findings reveal that, under constant light exposure, the mass element exhibits a robust self-sliding response, indicating its potential for use in energy harvesting and other applications that require sustained periodic motion. Additionally, this system can be extended to other non-circular curved tracks, highlighting its adaptability and versatility.
Effect of Aminating Lignin Loading with Arbuscular Mycorrhizal Fungi on Soil Aggregate Structure Improvement
Authors: Hu Chenghui , Xu Tingting , Wang Shumei , Bian Huiyang , Dai Hongqi
Published: June 2024

Lignin is an important component of plant fiber raw materials, and is a three-dimensional network structure aromatic polymer with abundant resources and a complex structure in nature. Lignin is generally used as industrial waste, and its potential value has not been fully utilized. Modern agriculture extensively uses chemical fertilizers, leading to the gradual degradation of soil fertility and structure, which seriously affects crop growth, nutrient transport, and root respiration function. Based on soil bulk density, porosity, aggregates, and their stability indicators, this study analyzed the effects of aminated industrial lignin and its loading with arbuscular mycorrhizal fungi on soil structure improvement and plant growth. It was hoped that resource-rich lignin could play a beneficial role in improving soil structure and promoting crop growth. The phenolic hydroxyl group of lignin was epoxidized and further aminated to load with arbuscular mycorrhizal fungi. The results indicated that amine-modified lignin could effectively load with arbuscular mycorrhizal fungi. The application of arbuscular mycorrhizal fungi-supported aminated lignin to soil aggregate structure improvement greatly reduced the bulk density of soil, and increased the porosity of soil and the content of large granular soil. Compared with unmodified soil, soil bulk density decreased by 73.08%, the porosity of soil increased by 70.43%, and the content of large granular soil increased by 56.38%. Using the improved soil for corn cultivation efficiently increased the biomass of corn. The plant height was increased by 72.16%, the root–shoot ratio was increased by 156.25%, and other indexes were also improved to varying degrees. The experimental method provides an important basis for the effective utilization of lignin materials in agriculture in the future.
Secular Religiosity: Heretical Imperative, Jewish Imponderables
Authors: Mendes-Flohr Paul Robert
Published: June 2024

The article develops a concept of “secular religiosity” to characterize “post-traditional” Jewish affiliation as an individual and private matter, which the sociologist Peter Berger casts as a “heretical imperative” to make autonomous, individual choices. The waning of the heteronomous authority of rabbinic Judaism, yielded theological and hermeneutic strategies to address the “secular religiosity” of individuals who sought to affirm distinctive Jewish spiritual and devotional practices. The article concludes by adumbrating two contrasting paradigmatic strategies exemplified by Franz Rosenzweig and Martin Buber respectively.
Barth’s “Alternative” Follower: Stanley Hauerwas and the Traditions of 20th-Century North American Theology and Ethics
Authors: Cui Renzhong , Han Siyi
Published: June 2024

This paper explores Stanley Hauerwas’s unique perspective on the traditions of 20th-century North American theology and ethics, particularly his similarity to Karl Barth in viewing theology and ethics as inseparable. Although deeply influenced by Barth, Hauerwas defends this view in the American context in a manner distinct from Barth‘s theological approach. Additionally, Hauerwas critiques Barth’s ecclesiology, which leads him to attempt to transcend some limitations of Barthian theology by developing a theological ethics “system” that emphasizes the practicality of the church.
Adversarial Attacks on Intrusion Detection Systems in In-Vehicle Networks of Connected and Autonomous Vehicles
Authors: Aloraini Fatimah , Javed Amir , Rana Omer
Published: June 2024

Rapid advancements in connected and autonomous vehicles (CAVs) are fueled by breakthroughs in machine learning, yet they encounter significant risks from adversarial attacks. This study explores the vulnerabilities of machine learning-based intrusion detection systems (IDSs) within in-vehicle networks (IVNs) to adversarial attacks, shifting focus from the common research on manipulating CAV perception models. Considering the relatively simple nature of IVN data, we assess the susceptibility of IVN-based IDSs to manipulation—a crucial examination, as adversarial attacks typically exploit complexity. We propose an adversarial attack method using a substitute IDS trained with data from the onboard diagnostic port. In conducting these attacks under black-box conditions while adhering to realistic IVN traffic constraints, our method seeks to deceive the IDS into misclassifying both normal-to-malicious and malicious-to-normal cases. Evaluations on two IDS models—a baseline IDS and a state-of-the-art model, MTH-IDS—demonstrated substantial vulnerability, decreasing the F1 scores from 95% to 38% and from 97% to 79%, respectively. Notably, inducing false alarms proved particularly effective as an adversarial strategy, undermining user trust in the defense mechanism. Despite the simplicity of IVN-based IDSs, our findings reveal critical vulnerabilities that could threaten vehicle safety and necessitate careful consideration in the development of IVN-based IDSs and in formulating responses to the IDSs’ alarms.
Limited Memory-Based Random-Weighted Kalman Filter
Authors: Gao Zhaohui , Zong Hua , Zhong Yongmin , Gao Guangle
Published: June 2024

The Kalman filter is an important technique for system state estimation. It requires the exact knowledge of system noise statistics to achieve optimal state estimation. However, in practice, this knowledge is often unknown or inaccurate due to uncertainties and disturbances involved in the dynamic environment, leading to degraded or even divergent filtering solutions. To address this issue, this paper presents a new method by combining the random weighting concept with the limited memory technique to accurately estimate system noise statistics. To avoid the influence of excessive historical information on state estimation, random weighting theories are established based on the limited memory technique to estimate both process noise and measurement noise statistics within a limited memory. Subsequently, the estimated system noise statistics are fed back into the Kalman filtering process for system state estimation. The proposed method improves the Kalman filtering accuracy by adaptively adjusting the weights of system noise statistics within a limited memory to suppress the interference of system noise on system state estimation. Simulations and experiments as well as comparison analysis were conducted, demonstrating that the proposed method can overcome the disadvantage of the traditional limited memory filter, leading to im-proved accuracy for system state estimation.
Anisotropic Sensing Performance in a High-Sensitivity Surface Plasmon Resonance Sensor Based on Few-Layer Black Phosphorus
Authors: Zhu Qifeng , Shen Yanting , Chen Zhuo , Chen Binghuang , Dai Enwen , Pan Weiqing
Published: June 2024

Few-layer black phosphorus (FLBP) is a highly promising material for high sensitivity label-free surface plasmon resonance (SPR) sensors due to its exceptional electrical, optical, and mechanical properties. FLBP exhibits inherent anisotropy with different refractive indices along its two main crystal orientations, the zigzag and armchair axes. However, this anisotropic property is often overlooked in FLBP-based sensors. In this study, we conducted a comprehensive investigation of the SPR reflectivity and phase in a BK7-Ag-FLBP structure to understand the influence of the stacking sequence and the number of FLBP layers on the sensing performance. Clear resonant angle shifts caused by different stacking sequences of FLBP could be observed both theoretically and experimentally. In the theoretical study, the highest reflective and phase sensitivities were achieved with a 12-layer black phosphorus (BP) structure. The reflectivity sensitivity reached 287.9°/refractive index units (RIU) with the zz stacking 12-layer BP film exhibiting a sensitivity 76°/RIU higher than the ac stacking structure. Similarly, the phase sensitivity reached 1162°/RIU with the zz stacking 12-layer BP structure showing a sensitivity 276.9°/RIU higher than the ac stacking structure. The electric field distribution of the 12-layer BP structure with four different stacking sequences has also been analyzed. In the experiment study, the well-known Attenuated Total Reflection (ATR) θ−2θ SPR setup is utilized to detect the reflectivity and phase of BK7-Ag-FLBP structures. The FLBP samples with the same thickness but different stacking sequences show significant resonant angle shift (0.275°) and maximum phase difference variation (34.6°). The FLBP sample thickness and crystal orientations have been demonstrated using the angular-resolved polarized Raman spectroscopy (ARPRS). These theoretical and experimental results provide strong evidence that the stacking sequences of FLBP have a significant impact on the sensing performance of SPR sensors. By harnessing the anisotropic properties of materials like FLBP, novel structures of anisotropic-2D material-based SPR sensors could open up exciting possibilities for innovative applications.
A Simple Method to Manufacture a Force Sensor Array Based on a Single-Material 3D-Printed Piezoresistive Foam and Metal Coating
Authors: Humbert Claude , Barriol Mathis , Varsavas Sakine Deniz , Nicolay Pascal , Brandstötter Mathias
Published: June 2024

Nowadays, 3D printing is becoming an increasingly common option for the manufacturing of sensors, primarily due to its capacity to produce intricate geometric shapes. However, a significant challenge persists in integrating multiple materials during printing, for various reasons. In this study, we propose a straightforward approach that combines 3D printing with metal coating to create an array of resistive force sensors from a single material. The core concept involves printing a sensing element using a conductive material and subsequently separating it into distinct parts using metal-coated lines connected to the electrical ground. This post-printing separation process involves manual intervention utilizing a stencil and metallic spray. The primary obstacle lies in establishing a sufficient contact surface between the sprayed metal and the structure, to ensure effective isolation among different zones. To address this challenge, we suggest employing a lattice structure to augment the contact surface area. Through experimental validation, we demonstrate the feasibility of fabricating two sensing elements from a single-material 3D-printed structure, with a maximum electrical isolation ratio between the sensors of above 30. These findings hold promise for the development of a new generation of low-tech 3D-printed force/displacement sensor arrays.
A Recursive Prediction-Based Feature Enhancement for Small Object Detection
Authors: Xiao Xiang , Xue Xiaorong , Zhao Zhiyuan , Fan Yisheng
Published: June 2024

Transformer-based methodologies in object detection have recently piqued considerable interest and have produced impressive results. DETR, an end-to-end object detection framework, ingeniously integrates the Transformer architecture, traditionally used in NLP, into computer vision for sequence-to-sequence prediction. Its enhanced variant, DINO, featuring improved denoising anchor boxes, has showcased remarkable performance on the COCO val2017 dataset. However, it often encounters challenges when applied to scenarios involving small object detection. Thus, we propose an innovative method for feature enhancement tailored to recursive prediction tasks, with a particular emphasis on augmenting small object detection performance. It primarily involves three enhancements: refining the backbone to favor feature maps that are more sensitive to small targets, incrementally augmenting the number of queries for small objects, and advancing the loss function for better performance. Specifically, The study incorporated the Switchable Atrous Convolution (SAC) mechanism, which features adaptable dilated convolutions, to increment the receptive field and thus elevate the innate feature extraction capabilities of the primary network concerning diminutive objects. Subsequently, a Recursive Small Object Prediction (RSP) module was designed to enhance the feature extraction of the prediction head for more precise network operations. Finally, the loss function was augmented with the Normalized Wasserstein Distance (NWD) metric, tailoring the loss function to suit small object detection better. The efficacy of the proposed model is empirically confirmed via testing on the VISDRONE2019 dataset. The comprehensive array of experiments indicates that our proposed model outperforms the extant DINO model in terms of average precision (AP) small object detection.
Optimizing Occupant Comfort in a Room Using the Predictive Control Model as a Thermal Control Strategy
Authors: Boicu Mihaela-Gabriela , Stamatescu Grigore , Făgărăşan Ioana , Vasluianu Mihaela , Neculoiu Giorgian , Dobrea Marius-Alexandru
Published: June 2024

Thermal comfort strategies represent a very important aspect when it comes to achieving thermal comfort conditions. At the same time, recently, there has been a growing interest in user-centered building control concepts. Thus, this work focuses on developing a thermal control strategy that combines the restrictions related to achieving thermal comfort, expressed in terms of environmental parameters and specific factors of personal perception, with the objective of reducing energy consumption. This case study aims at implementing this strategy in a laboratory room located within the Technical University of Civil Engineering Bucharest. The strategy proposed by the authors is based on implementing a combination of a Model Predictive Control (MPC) model and a fuzzy system, which presents constraints related to the room occupancy level. Relevant observations regarding the parameterization of fuzzy systems are also highlighted.
II–VI Semiconductor-Based Conductometric Gas Sensors: Is There a Future for These Sensors?
Authors: Korotcenkov Ghenadii
Published: June 2024

A review of the state of research in the development of conductometric gas sensors based on II–VI semiconductors is given. It was shown that II–VI compounds indeed have properties that are necessary for the development of highly efficient gas sensors. In this case, to achieve the required parameters, all approaches developed for metal oxides can be used. At the same time, during a detailed review, it was concluded that sensors based on II–VI compounds have no prospects for appearing on the gas sensor market. The main obstacle is the instability of the surface state, which leads to poor reproducibility of parameters and drift of sensor characteristics during operation.
Deformation Monitoring Based on SBAS-InSAR and Leveling Measurement: A Case Study of the Jing-Mi Diversion Canal in China
Authors: Luo Pengjun , Jin Xinxin , Nie Ding , Liu Youzhi , Wei Yilun
Published: June 2024

The Jing-Mi Diversion Canal is a large-scale water diversion project in Beijing. Routine monitoring is crucial for the reliability and stability of urban water supply. Compared with traditional monitoring methods, interferometric synthetic aperture radar (InSAR) has the advantages of large scale and high accuracy. Based on the small baseline subset InSAR, 187 ascending and 102 descending SAR images obtained from Sentinel-1 were used to detect the deformation along the diversion canal from 2017 to 2023. The results show that there was a sinking trend along the diversion canal. The subsidence was serious in the first half of the canal, and continued to sink from 2019 to 2020. The subsidence was alleviated in 2023. Combined with leveling measurements, the InSAR deformation monitoring results of important pumping station buildings were verified. The measurement accuracy of InSAR can reach the millimeter level. We extracted the groundwater level time series and subsidence for risky canal segments. Through pixel-by-pixel comparison, it was found that fluctuations in groundwater level would have some impact on surface deformation. Severe local subsidence or uplift deformation occasionally occurred. To ensure the safety of water diversion, the monitoring and maintenance of relevant pump station buildings in risky areas should be increased in the future.
Exploring Perception of Warning Labels: Insights from Color, Signal Words, and Symbol Evaluation
Authors: Gopang Miskeen Ali , Ali Tauha Hussain , Shaikh Shakeel Ahmed
Published: June 2024

Protecting people from the risks associated with products is a critical concern in today’s economy. Pakistan, being the world’s fifth most populous country, lacks the framework of warning labels and therefore faces a significant gap in product warning labels. Pakistan is a representative of a number of countries that export a variety of products to Pakistan; however, warning labels on these goods are typically in English, which might mislead people of Pakistan in perceiving the hazard level. It is therefore imperative to conduct research into the non-textual and cross-cultural understanding of labels from the perspective of Pakistan. This study examined the applicability of ANSI Z535.4 in the context of Pakistan. A total of 66 (34 male and 32 female) undergraduate students with a mean age of 20.5 participated in this study. A meticulous experiment was designed using a nine-point rating scale with anchors on both sides, where one represented ‘not at all hazardous’ and nine represented ‘extremely hazardous’. Participants rated each component of warning labels, i.e., color, symbol, signal words, and their complex configurations. The results showed alignment with the ANSI Z535.4 standards for some components (i.e., colors, symbols, and signal words) and complex configurations, whereas no significant difference was found in perceived hazard levels between green (M = 3.167), blue (M = 3.591, and yellow (M = 3.652) colors, with a p-value greater than 0.05. Participants did not differentiate significantly between signal words, i.e., caution (M = 5.182) and warning (M = 5.879). Participants also did not differentiate significantly between complex configurations, i.e., safety alert–caution–yellow (M = 5.076) and safety alert–warning–orange (M = 5.197), with p-values greater than 0.05. These results state that discrepancies in the perception of warning labels exist. This study is the first of its kind conducted in the context of Pakistan, which will help policy makers to consider the findings before implementing a policy. In fact, differences in perception could result in failure to take appropriate precautions. Nonetheless, these nuances can be overcome with proper awareness through training for the people.
The Relationship between Adult Attachment Style and Perceived Knowledge of Partner Sex History among University Students
Authors: Martins Mendonca Victoria , Lasenby-Lessard Jennifer , Davies Adam
Published: June 2024

Research has extensively studied adult attachment theory, a fundamental psychological framework, particularly regarding its implications for adult attachment and sexual dynamics. The previous literature has established relationships between adult attachment and various sexual behaviours as well as communication patterns. Specifically, individuals with secure attachment styles typically engage in open communication and cautious sexual behaviours, whereas individuals with anxious or avoidant attachment styles exhibit less sexual communication and engage in riskier sexual activities. That said, limited research has explored the relationship between adult attachment style and perceived knowledge regarding partner sex history. This study aimed to fill this gap by investigating how adult attachment style relates to perceived knowledge about partner sexual experiences. A sample of 237 undergraduate students completed surveys assessing attachment style and their perceived awareness of their partner’s sexual past. Attachment style was evaluated using the Revised Adult Attachment Scale (RAAS) and perceived knowledge was measured using the Partner Sexual History Scale (PSHS). The results indicated a significant correlation between adult attachment style and perceived knowledge of a partner’s sexual history. Specifically, individuals with secure and avoidant attachment styles demonstrated greater perceived awareness of their partner’s sexual experiences compared to those with an anxious attachment style. This research contributes to our comprehension of the complex interplay among adult attachment, sexual knowledge, sexual communication, and relationship dynamics in young adult populations.
Ciliated Protist Communities in Soil: Contrasting Patterns in Natural Sites and Arable Lands across Italy
Authors: Bharti Daizy , Kumar Santosh , Basuri Charan Kumar , La Terza Antonietta
Published: June 2024

This study represents the first investigation of soil ciliate diversity and community structure in the Marche region, Italy, encompassing both natural sites and agro-ecosystems. The main aims were (i) to assess the ability of ciliates to discriminate between different types of land uses, i.e., arable lands and possible farming management practices [organic (ORG) vs. conventional (CON)], and forest (FOR) sites; and (ii) to investigate the relationships among ciliate communities and abiotic parameters at the studied sites. Soil samples were collected twice from 10 sites (5 forest (FOR) (natural soils) and 5 arable lands under different agricultural management systems (3 ORG (minimum tillage) and 2 CON (sod seeding)). Ciliate communities were studied using qualitative (non-flooded Petri dish) and quantitative methods (ciliate counts from permanent slides). Soil chemical–physical (texture, CEC, N, OM, C/N) parameters were also measured. Qualitative ciliate analysis allowed us to identify a total of 59 species representing 33 genera, 20 families, 13 orders, and 7 classes. ORG sites were the richest in species followed by CON and FOR. Multivariate analysis showed statistically significant differences between natural sites (FOR) and agricultural sites, and between ORG and CON management farming systems. CCA analysis revealed a positive correlation between the ciliate species and silt, clay, and pH in ORG sites, and sand, organic carbon, organic matter, total nitrogen, C/N ratio, and CEC (cation exchange capacity) in FOR sites, suggesting the significance of these parameters in shaping the ciliate communities. Altogether, these results showed the bioindicative potential of ciliate communities in discriminating between natural sites (FOR) and arable lands, and their capacity to discriminate, at least preliminarily, between different soil management systems (ORG vs. CON). Furthermore, this study highlights the high diversity of soil ciliates and their response to habitat variability.
Current Trends in Sustainable Sewage Sludge Management—A Case Study for Poznań County, Poland
Authors: Jakubus Monika
Published: June 2024

The formation of sewage sludge (SS) is directly related to the number of people served by municipal wastewater treatment plants (WWTPs), while it is also impacted by increasingly upgraded sewage treatment technologies used in such installations. The presence of SS in our environment raises a number of concerns regarding its proper and sustainable management. In practice, the choice of a specific SS disposal method is determined by a number of factors and, as this research has shown, it may vary significantly both on the national and regional scale. This study analyzed this phenomenon in relation to Poznań County, located in the Wielkopolskie province, Poland. As part of this study, the SS chemical composition was assessed based on data obtained directly from local municipal WWTPs over the course of several years (the last 7 years). The currently used SS management methods were analyzed in terms of SS chemical composition. Moreover, in view of the importance of public involvement in decision-making processes related to sustainable management of SS, an original survey was developed to assess local residents’ knowledge concerning SS management in Poznań County. Based on the obtained data, it was found that the generated SS, constituting a form of fertilizer, was primarily used for agricultural and reclamation purposes (over 41% of total SS mass in the case of Poznań County). It is facilitated by the chemical composition of this waste, characterized by high contents of organic matter (380–730.3 g·kg−1), nitrogen (13.3–78 g·kg−1), and calcium (9.5–350 g·kg−1), while the amounts of heavy metals were within the permissible ranges. A survey of the local community revealed that although respondents recognized and could accurately identify SS as a waste, their knowledge concerning harmfulness of this waste and its management was fragmentary. Regardless of the age of the respondents, 48 to 66% of them had no opinion concerning problems related to SS in their area. The youngest people (aged 18–25) showed the lowest level of knowledge on SS and its management. People with elementary education were the least knowledgeable about sewage sludge management. Regardless of the above, a majority of respondents (28–56% for different age groups and 7–18% for various educational backgrounds) were convinced of the validity of agricultural SS use. To sum up, research on sewage sludge is an indispensable element of activities aimed at sustainable development, combining aspects of environmental protection, circular economy, and social awareness and acceptance. Additionally, the study results indicated the need for social education to increase environmental awareness and co-responsibility for SS management.