bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of homeotropic alignment strength at the air interface of polymerized liquid crystal layers
Authors: Xue Xiangyu , Berteloot Brecht , Stebryte Migle , Neyts Kristiaan , Beeckman Jeroen
Published: November 2021

Liquid crystal thin films obtained by spincoating and photopolymerization are widely used nowadays for geometric phase optical components. The liquid crystal-air interface during photopolymerization plays a crucial role as it should not disturb the photoalignment induced by the other interface. When photopolymerizing the liquid crystal layer in vacuum, processing parameters need to be optimized carefully to avoid alignment issues at the air interface. In this work we demonstrate that domains are formed due to a tilt angle which is induced at the air interface under certain conditions. A measurement method is developed to quantify the tilt angle at the interface. The method consists of optical analysis of the domains using crossed-polarizer transmission microscopy with an oblique sample holder. By careful analysis of the obtained tilt angles for different parameters, it is assumed that a non-negligible homeotropic anchoring strength at the air interface is responsible for the domain formation. The results are explained with a theoretical model that assumes a finite anchoring strength at both the photoaligned interface and the air interface.
Dynamic divisive normalization circuits explain and predict change detection in monkey area MT
Authors: Ernst Udo A. , Chen Xiao , Bohnenkamp Lisa , Galashan Fingal Orlando , Wegener Detlef
Published: November 2021

Sudden changes in visual scenes often indicate important events for behavior. For their quick and reliable detection, the brain must be capable to process these changes as independently as possible from its current activation state. In motion-selective area MT, neurons respond to instantaneous speed changes with pronounced transients, often far exceeding the expected response as derived from their speed tuning profile. We here show that this complex, non-linear behavior emerges from the combined temporal dynamics of excitation and divisive inhibition, and provide a comprehensive mathematical analysis. A central prediction derived from this investigation is that attention increases the steepness of the transient response irrespective of the activation state prior to a stimulus change, and irrespective of the sign of the change (i.e. irrespective of whether the stimulus is accelerating or decelerating). Extracellular recordings of attention-dependent representation of both speed increments and decrements confirmed this prediction and suggest that improved change detection derives from basic computations in a canonical cortical circuitry.
Targeted chromosomal Escherichia coli:dnaB exterior surface residues regulate DNA helicase behavior to maintain genomic stability and organismal fitness
Authors: Behrmann Megan S. , Perera Himasha M. , Hoang Joy M. , Venkat Trisha A. , Visser Bryan J. , Bates David , Trakselis Michael A.
Published: November 2021

Helicase regulation involves modulation of unwinding speed to maintain coordination of DNA replication fork activities and is vital for replisome progression. Currently, mechanisms for helicase regulation that involve interactions with both DNA strands through a steric exclusion and wrapping (SEW) model and conformational shifts between dilated and constricted states have been examined in vitro. To better understand the mechanism and cellular impact of helicase regulation, we used CRISPR-Cas9 genome editing to study four previously identified SEW-deficient mutants of the bacterial replicative helicase DnaB. We discovered that these four SEW mutations stabilize constricted states, with more fully constricted mutants having a generally greater impact on genomic stress, suggesting a dynamic model for helicase regulation that involves both excluded strand interactions and conformational states. These dnaB mutations result in increased chromosome complexities, less stable genomes, and ultimately less viable and fit strains. Specifically, dnaB:mut strains present with increased mutational frequencies without significantly inducing SOS, consistent with leaving single-strand gaps in the genome during replication that are subsequently filled with lower fidelity. This work explores the genomic impacts of helicase dysregulation in vivo, supporting a combined dynamic regulatory mechanism involving a spectrum of DnaB conformational changes and relates current mechanistic understanding to functional helicase behavior at the replication fork.
Knowledge, attitude and reported practice regarding donning and doffing of personal protective equipment among frontline healthcare workers against COVID-19 in Nepal: A cross-sectional study
Authors: Pandey Sagar , Poudel Sujan , Gaire Ashok , Poudel Ritu , Subedi Prabin , Gurung Jyoti , Sharma Rituraj , Thapa Jeevan
Published: November 2021

Background

Coronavirus Disease 2019 (COVID-19) is a respiratory infection with a high rate of transmission primarily via airborne route and direct contact. Proper use of personal protective equipment (PPE) is a proven and effective way to prevent COVID-19 spread in healthcare settings. This study was done aiming to assess the knowledge, attitude, and reported practice, and identify the associated factors regarding donning and doffing of PPE among frontline healthcare workers in Nepal.

Methods

A cross-sectional study was conducted from 25th April to 30th July 2021 among 205 frontline healthcare workers of Nepal selected randomly from among the contacts of the investigators. A structured self-administered questionnaire prepared in google form was used as a study tool and shared via social media to the participants to obtain information on socio-demographic and workplace characteristics along with their knowledge, attitude, and reported practice regarding donning and doffing of PPE.

Result

A total of 79.5% of participants had satisfactory knowledge while 75.6% had satisfactory practice scores regarding donning and doffing of PPE. Factors such as the profession of the participants (p-value = 0.048), their workplace (p-value = 0.005), provision of PPE at workplace (p-value = 0 .009), and availability of designated space at workplace for methodical donning and doffing of PPE (p-value = 0.010) were significantly associated with satisfactory knowledge score whereas availability of designated space at workplace for donning and doffing of PPE was significantly associated with good practice score (p-value = 0.009).

Conclusion

This study demonstrated an overall good knowledge, attitude, and reported practice regarding donning and doffing of PPE among frontline healthcare workers in Nepal. However, the reported shortcomings like poor knowledge regarding the sequence of donning and doffing and corresponding flawed practice behaviors need to be addressed.
Seroprevalence of anti-SARS-CoV-2 IgG at the first epidemic peak in French Guiana, July 2020
Authors: Flamand Claude , Alves Sarmento Christelle , Enfissi Antoine , Bailly Sarah , Beillard Emmanuel , Gaillet Mélanie , Michaud Céline , Servas Véronique , Clement Nathalie , Perilhou Anaïs , Carage Thierry , Musso Didier , Carod Jean-françois , Eustache Stéphanie , Tourbillon Céline , Boizon Elodie , James Samantha , Djossou Félix , Salje Henrik , Cauchemez Simon , Rousset Dominique
Published: November 2021

Background

While Latin America has been heavily affected by the pandemic, only a few seroprevalence studies have been conducted there during the first epidemic wave in the first half of 2020.

Methodology/Principal findings

A cross-sectional survey was performed between 15 July 2020 and 23 July 2020 among individuals who visited 4 medical laboratories or 5 health centers for routine screening or clinical management, with the exception of symptomatic suggestive cases of covid-19.

Samples were screened for the presence of anti-SARS-CoV-2 IgG directed against domain S1 of the SARS-CoV-2 spike protein using the anti-SARS-CoV-2 enzyme-linked immunosorbent assay (ELISA) from Euroimmun.

Conclusions/Significance

The overall seroprevalence was 15.4% [9.3%-24.4%] among 480 participants, ranging from 4.0% to 25.5% across the different municipalities. The seroprevalence did not differ according to gender (p = 0.19) or age (p = 0.51). Among SARS-CoV-2 positive individuals, we found that 24.6% [11.5%-45.2%] reported symptoms consistent with COVID-19. Our findings revealed high levels of infection across the territory but a low number of resulting deaths, which can be explained by French Guiana’s young population structure.
Mind training, stress and behaviour—A randomised experiment
Authors: Alem Yonas , Behrendt Hannah , Belot Michèle , Bíró Anikó
Published: November 2021

In this paper, we evaluate the effects of a psychological training, called Mindfulness-Based Stress Reduction (MBSR) on stress and risk and time preferences. MBSR is a well-known psychological technique, which is believed to improve self-control and reduce stress. We conduct the experiment with 139 participants, half of whom receive the MBSR training, while the other half are asked to watch a documentary series, both over 4 consecutive weeks. Using a range of self-reported and physiological measures (such as cortisol measures), we find evidence that mindfulness training reduces perceived stress, but we only find weak evidence of effects on risk and inter-temporal attitudes.
External validation of EPIC’s Risk of Unplanned Readmission model, the LACE+ index and SQLape as predictors of unplanned hospital readmissions: A monocentric, retrospective, diagnostic cohort study in Switzerland
Authors: Hwang Aljoscha Benjamin , Schuepfer Guido , Pietrini Mario , Boes Stefan
Published: November 2021

Introduction

Readmissions after an acute care hospitalization are relatively common, costly to the health care system, and are associated with significant burden for patients. As one way to reduce costs and simultaneously improve quality of care, hospital readmissions receive increasing interest from policy makers. It is only relatively recently that strategies were developed with the specific aim of reducing unplanned readmissions using prediction models to identify patients at risk. EPIC’s Risk of Unplanned Readmission model promises superior performance. However, it has only been validated for the US setting. Therefore, the main objective of this study is to externally validate the EPIC’s Risk of Unplanned Readmission model and to compare it to the internationally, widely used LACE+ index, and the SQLAPE® tool, a Swiss national quality of care indicator.

Methods

A monocentric, retrospective, diagnostic cohort study was conducted. The study included inpatients, who were discharged between the 1stof January 2018 and the 31stof December 2019 from the Lucerne Cantonal Hospital, a tertiary-care provider in Central Switzerland. The study endpoint was an unplanned 30-day readmission. Models were replicated using the original intercept and beta coefficients as reported. Otherwise, score generator provided by the developers were used. For external validation, discrimination of the scores under investigation were assessed by calculating the area under the receiver operating characteristics curves (AUC). Calibration was assessed with the Hosmer-LemeshowX2goodness-of-fit test This report adheres to the TRIPOD statement for reporting of prediction models.

Results

At least 23,116 records were included. For discrimination, the EPIC´s prediction model, the LACE+ index and the SQLape® had AUCs of 0.692 (95% CI 0.676–0.708), 0.703 (95% CI 0.687–0.719) and 0.705 (95% CI 0.690–0.720). The Hosmer-LemeshowX2tests had values of p<0.001.

Conclusion

In summary, the EPIC´s model showed less favorable performance than its comparators. It may be assumed with caution that the EPIC´s model complexity has hampered its wide generalizability—model updating is warranted.
Enhanced serodiagnostic potential of a fusion molecule consisting of Rv1793, Rv2628 and a truncated Rv2608 of Mycobacterium tuberculosis
Authors: Sulman Sadaf , Shahid Saher , Khaliq Aasia , Ambreen Atiqa , Khan Imran H. , Cooper Andrea M. , Akhtar Muhammad Waheed
Published: November 2021

Serodiagnosis of tuberculosis (TB) can be rapid, reliable and cost-effective if the issue of variable antibody responses of TB patients against differentMycobacterium tuberculosis(Mtb) antigens can be overcome by developing fusion proteins containing epitopes from multiple antigens ofMtb. In this study,Mtbantigens Rv1793, Rv2628, Rv2608 and a truncated variant produced by removing non-epitopic region from N-terminal of Rv2608 (tnRv2608), and the fusion protein Rv1793-Rv2628-tnRv2608 (TriFu64), were expressed inE.coliand purified. Plasma samples from TB patients characterized by sex, age and sputum/culture positivity, were used to compare the sensitivity of the single antigens with the fusion protein. Sensitivity of Rv1793, Rv2628 and Rv2608, was 27.8%, 39% and 36.3%, respectively. Truncation of Rv2608 increased sensitivity by approximately 35% in confirmed TB cases. Sensitivity of the fusion construct, TriFu64 increased to 66% with a specificity of 100%. Importantly, tnRv2608 was better able to detect sputum and culture negative patients, and this carried through to the fusion protein. We demonstrate that fusion ofMtbproteins ensures broad sensitivity across disease types, sex and age groups in a Pakistani population.
Unravelling adolescent girls’ aspirations in Nepal: Status and associations with individual-, household-, and community-level characteristics
Authors: Madjdian Dónya S. , Cunningham Kenda , Bras Hilde , Koelen Maria , Vaandrager Lenneke , Adhikari Ramesh P. , Talsma Elise F.
Published: November 2021

Background

Adolescents’ aspirations have potential to influence their present and future well-being. Limited knowledge exists on adolescent girls’ aspirations and their determinants, particularly in low-income contexts.

Methods and findings

Using cross-sectional data, collected in 2018 in Nepal, within theSuaahara IIAdolescent Panel Survey, (n = 840), adolescent girls’ aspirations in several domains—education, occupation, marriage, fertility, health, and nutrition–were described. Regression models were estimated to explore associations between individual, household and community characteristics and these aspirations for all adolescents and separately for younger (10–14 years) and older (15–19 years) girls. Age, school attendance, and self-efficacy, as well as household wealth, caste/ethnicity, size, and agro-ecological zone of residence were significantly associated with aspirations, although effect sizes and significance varied by aspiration domain and age group.

Conclusions

Findings underscore the curtailing effect of poverty on aspirations and the dynamic nature of aspirations. Initiatives to foster girls’ aspirations must address both individual and contextual factors.
Clinical and molecular epidemiology of influenza viruses from Romanian patients hospitalized during the 2019/20 season
Authors: Miron Victor Daniel , Bănică Leontina , Săndulescu Oana , Paraschiv Simona , Surleac Marius , Florea Dragoș , Vlaicu Ovidiu , Milu Petre , Streinu-Cercel Anca , Bilașco Anuta , Oțelea Dan , Pițigoi Daniela , Streinu-Cercel Adrian , Drăgănescu Anca Cristina
Published: November 2021

Two main mechanisms contribute to the continuous evolution of influenza viruses: accumulation of mutations in the hemagglutinin and neuraminidase genes (antigenic drift) and genetic re-assortments (antigenic shift). Epidemiological surveillance is important in identifying new genetic variants of influenza viruses with potentially increased pathogenicity and transmissibility. In order to characterize the 2019/20 influenza epidemic in Romania, 1042 respiratory samples were collected from consecutive patients hospitalized with acute respiratory infections in the National Institute for Infectious Diseases “Prof. Dr. Matei Balș”, Bucharest Romania and tested for influenza A virus, influenza B virus and respiratory syncytial virus (RSV) by real-time PCR. Out of them, 516 cases were positive for influenza, with relatively equal distribution of influenza A and B. Two patients had influenza A and B co-infection and 8 patients had influenza-RSV co-infection. The most severe cases, requiring supplemental oxygen administration or intensive care, and the most deaths were reported in patients aged 65 years and over. Subtyping showed the predominance of A(H3N2) compared to A(H1N1)pdm09 pdm09 (60.4% and 39.6% of all subtyped influenza A isolates, respectively), and the circulation of Victoria B lineage only. Influenza B started to circulate first (week 47/2019), with influenza A appearing slightly later (week 50/2019), followed by continued co-circulation of A and B viruses throughout the season. Sixty-eight samples, selected to cover the entire influenza season and all circulating viral types, were analysed by next generation sequencing (NGS). All A(H1N1)pdm09 sequences identified during this season in Romania were clustered in the 6b1.A clade (sub-clades: 6b1.A.183P -5a and 6b1.A.187A). For most A(H1N1)pdm09 sequences, the dominant epitope was Sb (pepitope = 0.25), reducing the vaccine efficacy by approximately 60%. According to phylogenetic analysis, influenza A(H3N2) strains circulating in this season belonged predominantly to clade 3C.3A, with only few sequences in clade 3C.2A1b. These 3C.2A1b sequences, two of which belonged to vaccinated patients, harbored mutations in antigenic sites leading to potential reduction of vaccine efficacy. Phylogenetic analysis of influenza B, lineage Victoria, sequences showed that the circulating strains belonged to clade V1A3. As compared to the other viral types, fewer mutations were observed in B/Victoria strains, with limited impact on vaccine efficiency based on estimations.
Predictors of school students’ leisure-time physical activity: An extended trans-contextual model using Bayesian path analysis
Authors: Polet Juho , Schneider Jekaterina , Hassandra Mary , Lintunen Taru , Laukkanen Arto , Hankonen Nelli , Hirvensalo Mirja , Tammelin Tuija H. , Hamilton Kyra , Hagger Martin S.
Published: November 2021

The present study aimed to examine effects of motivational and social cognition constructs on children’s leisure-time physical activity participation alongside constructs representing implicit processes using an extended trans-contextual model. The study adopted a correlational prospective design. Secondary-school students (N= 502) completed self-report measures of perceived autonomy support from physical education (PE) teachers, autonomous motivation in PE and leisure-time contexts, and social cognition constructs (attitudes, subjective norms, perceived behavioral control), intentions, trait self-control, habits, and past behavior in a leisure-time physical activity context. Five weeks later, students (N= 298) self-reported their leisure-time physical activity participation. Bayesian path analyses supported two key premises of the model: perceived autonomy support was related to autonomous motivation in PE, and autonomous motivation in PE was related to autonomous motivation in leisure time. Indirect effects indicated that both forms of autonomous motivation were related to social cognition constructs and intentions. However, intention was not related to leisure-time physical activity participation, so model variables reflecting motivational processes did not account for substantive variance in physical activity participation. Self-control, attitudes, and past behavior were direct predictors of intentions and leisure-time physical activity participation. There were indirect effects of autonomous motivation in leisure time on intentions and physical activity participation mediated by self-control. Specifying informative priors for key model relations using Bayesian analysis yielded greater precision for some model effects. Findings raise some questions on the predictive validity of constructs from the original trans-contextual model in the current sample, but highlight the value of extending the model to incorporate additional constructs representing non-conscious processes.
Efficacy and safety of manual acupuncture for the treatment of upper limb motor dysfunction after stroke: Protocol for a systematic review and meta-analysis
Authors: Cao Di , Zhang Xiaolin , Liu Mingjun , Yang Qiguang , Gu Shuhong , Gao Tianjiao , Cong Lin , Ma Dehui , Lin Hongju , Chen Shaotao
Published: November 2021

Introduction

The incidence of stroke sequelae among patients is as high as 70%–80%. Flexor spasm is the most common stroke sequela, presenting a heavy burden to the patients and their families. This study will evaluate the results of randomized controlled trials to determine the efficacy and safety of hand manipulation acupuncture for the treatment of upper limb motor dysfunction after stroke.

Methods

Eight databases, including China National Knowledge Infrastructure, Chinese Scientific Journal Database, Cochrane Central Register of Controlled Trials, Embase, MEDLINE, PubMed, Wanfang Database, and Web of Science, will be searched using English and Chinese search strategies. In addition, manual retrieval of research papers, conference papers, ongoing experiments, and internal reports, among others, will supplement electronic retrieval. All eligible studies published on or before January 15, 2021 will be selected. To enhance the effectiveness of the study, only clinical randomized controlled trials related to the use of manual acupuncture for the treatment of upper limb motor dysfunction after stroke will be included.

Analysis

The Fugl-Meyer upper extremity assessment will be the primary outcome measure, whereas the Wolf Motor Function Test, Modified Ashworth Scale, arm movement survey test table, and upper extremity freehand muscle strength assessment scores will be the secondary outcomes. Side effects and adverse events will be included as safety evaluations. To ensure the quality of the systematic evaluation, study selection, data extraction, and quality assessment will be independently performed by two authors, and a third author will resolve any disagreement.

Ethics and dissemination

This systematic review will evaluate the efficacy and safety of manual acupuncture for the treatment of upper limb motor dysfunction after stroke. Since all included data will be obtained from published articles, it does not require ethical approval and will be published in a peer-reviewed journal.

INPLASY registration number: INPLASY202110071.
A social norms approach intervention to address misperceptions of anti-vaccine conspiracy beliefs amongst UK parents
Authors: Cookson Darel , Jolley Daniel , Dempsey Robert C. , Povey Rachel
Published: November 2021

Anti-vaccine conspiracy beliefs among parents can reduce vaccination intentions. Parents’ beliefs in anti-vaccine conspiracy theories are also related to their perceptions of other parents’ conspiracy beliefs. Further, research has shown that parents hold misperceptions of anti-vaccine conspiracy belief norms: UK parents over-estimate the anti-vaccine conspiracy beliefs of other parents. The present study tested the effectiveness of a Social Norms Approach intervention, which corrects misperceptions using normative feedback, to reduce UK parents’ anti-vaccine conspiracy beliefs and increase vaccination intentions. At baseline, 202 UK parents of young children reported their personal belief in anti-vaccine conspiracy theories, future intentions to vaccinate, and their perceptions of other UK parents’ beliefs and intentions. Participants were then randomly assigned to a normative feedback condition (n = 89) or an assessment-only control condition (n = 113). The normative feedback compared participants’ personal anti-vaccine conspiracy beliefs and perceptions of other UK parents’ beliefs with actual normative belief levels. Parents receiving the normative feedback showed significantly reduced personal belief in anti-vaccine conspiracy beliefs at immediate post-test. As hypothesised, changes in normative perceptions of anti-vaccine conspiracy beliefs mediated the effect of the intervention. The intervention, did not directly increase vaccination intentions, however mediation analysis showed that the normative feedback increased perceptions of other parents’ vaccination intentions, which in turn increased personal vaccination intentions. No significant effects remained after a six-week follow-up. The current research demonstrates the potential utility of Social Norms Approach interventions for correcting misperceptions and reducing anti-vaccine conspiracy beliefs among UK parents. Further research could explore utilising a top-up intervention to maintain the efficacy.
A novel probabilistic generator for large-scale gene association networks
Authors: Grimes Tyler , Datta Somnath
Published: November 2021

Motivation

Gene expression data provide an opportunity for reverse-engineering gene-gene associations using network inference methods. However, it is difficult to assess the performance of these methods because the true underlying network is unknown in real data. Current benchmarks address this problem by subsampling a known regulatory network to conduct simulations. But the topology of regulatory networks can vary greatly across organisms or tissues, and reference-based generators—such as GeneNetWeaver—are not designed to capture this heterogeneity. This means, for example, benchmark results from the E. coli regulatory network will not carry over to other organisms or tissues. In contrast, probabilistic generators do not require a reference network, and they have the potential to capture a rich distribution of topologies. This makes probabilistic generators an ideal approach for obtaining a robust benchmarking of network inference methods.

Results

We propose a novel probabilistic network generator that (1) provides an alternative to address the inherent limitation of reference-based generators and (2) is able to create realistic gene association networks, and (3) captures the heterogeneity found across gold-standard networks better than existing generators used in practice. Eight organism-specific and 12 human tissue-specific gold-standard association networks are considered. Several measures of global topology are used to determine the similarity of generated networks to the gold-standards. Along with demonstrating the variability of network structure across organisms and tissues, we show that the commonly used “scale-free” model is insufficient for replicating these structures.

Availability

This generator is implemented in the R package “SeqNet” and is available on CRAN (https://cran.r-project.org/web/packages/SeqNet/index.html).
Breastfeeding and weaning practices among mothers in Ghana: A population-based cross-sectional study
Authors: Appiah Prince Kubi , Amu Hubert , Osei Eric , Konlan Kennedy Diema , Mumuni Iddris Hadiru , Verner Orish Ndudiri , Maalman Raymond Saa-Eru , Kim Eunji , Kim Siwoo , Bukari Mohammed , Jung Hajun , Kofie Philip , Ayanore Martin Amogre , Amenuvegbe Gregory Kofi , Adjuik Martin , Tarkang Elvis Enowbeyang , Alhassan Robert Kaba , Donkor Ernestina Safoa , Zotor Francis Bruno , Kweku Margaret , Amuna Paul , Gyapong John Owusu , Kim So Yoon
Published: November 2021

Background

Children need good nutrition to develop proper immune mechanisms and psychosocial maturity, but malnutrition can affect their ability to realize this. Apart from the national demographic and health survey, which is carried out every 5 years, there have not been enough documented studies on child breastfeeding and weaning practices of caregivers in the Volta Region. We, therefore, examined child breastfeeding and weaning practices of mothers in the Volta Region of Ghana.

Methods

A sub-national survey method was adopted and a semi-structured questionnaire was used to collect data from 396 mothers and their children. Descriptive and inferential statistics comprising frequency, percentage, chi-square, and logistic regression were employed in analysing the data. We defined exclusive breastfeeding as given only breast milk to an infant from a mother or a wet nurse for six months of life except drops or syrups consisting of vitamins, minerals, supplements, or medicines on medical advice, and prolonged breastfeeding as breastfeeding up to 24 months of age.

Results

The prevalence of exclusive breastfeeding (EBF) was 43.7%. Mothers constituting 61.1% started breastfeeding within an hour of giving birth. In addition to breast milk, 5.1% gave fluids to their children on the first day of birth. About 66.4% started complementary feeding at 6 months, 22.0% breastfed for 24 months or beyond, while 40.4% fed their children on-demand. Child’s age (AOR: 0.23, 95% CI:0.12–0.43, p<0.0001), prolonged breastfeeding (AOR: 0.41, 95%CI: 0.12–0.87, p = 0.001), mother’s religion (AOR: 3.92, 95%CI: 1.23–12.61, p = 0.021), feeding practices counselled on (AOR: 1.72, 95%CI: 1.96–3.09, p = 0.023), mother ever heard about EBF (AOR: 0.43, 95%CI: 1.45–2.41,p= 0.039), child being fed from the bottle with a nipple (AOR: 1.53, 95%CI: 1.94–2.48,p= 0.003), and age at which complementary feeding was started (AOR: 17.43, 95%CI: 3.47–87.55,p= 0.008) were statistically associated with EBF.

Conclusion

Breastfeeding education has been ongoing for decades, yet there are still gaps in the breastfeeding practices of mothers. To accelerate progress towards attainment of the sustainable development goal 3 of ensuring healthy lives and promoting well-being for all at all ages by the year 2030, we recommend innovative policies that include extensive public education to improve upon the breastfeeding and weaning practices of mothers.
Effect of obstructive sleep apnea on cerebrovascular compliance and cerebral small vessel disease
Authors: Lee Woo-Jin , Jung Keun-Hwa , Nam Hyun-Woo , Lee Yong-Seok
Published: November 2021

Reduced cerebrovascular compliance is the major mechanism of cerebral small vessel disease (SVD). Obstructive sleep apnea (OSA) also promotes SVD development, but the underlying mechanism was not elucidated. We investigated the association among OSA, cerebrovascular compliance, and SVD parameters. This study retrospectively included individuals ≥ 50 years of age, underwent overnight polysomnographic (PSG) for the evaluation of OSA, and performed MRI and transcranial Doppler (TCD) within 12 months of interval without a neurological event between the evaluations. TCD parameters for the cerebrovascular compliance included middle cerebral artery pulsatility index (MCA PI) and mean MCA resistance index ratio (MRIR). SVD parameters included white matter hyperintensity (WMH) volume, number of lacunes, enlarged perivascular space (ePVS) score, and the presence of microbleeds or lacunes. Ninety-seven individuals (60.8% male, mean age 70.0±10.5 years) were included. MRIR was associated with higher respiratory distress index (B = 0.003; 95% confidence interval [CI] 0.001–0.005; P = 0.021), while MCA PI was not associated with any of the PSG markers for OSA severity. Apnea-hypopnea index was associated with the log-transformed total WMH volume (B = 0.008; 95% confidence interval [CI] 0.001–0.016; P = 0.020), subcortical WMH volume (B = 0.015; 95% CI 0.007–0.022; P<0.001), total ePVS score (B = 0.024; 95% CI 0.003–0.045; P = 0.026), and centrum semiovale ePVS score (B = 0.026; 95% CI 0.004–0.048; P = 0.019), and oxygen-desaturation index with periventricular WMH volume, independently from age, MCA PI, and MRIR. This study concluded that OSA is associated with reduced cerebrovascular compliance and also with SVD independently from cerebrovascular compliance. Underlying pathomechanistic link might be region specific.
COVID-19 vaccine hesitancy and refusal and associated factors in an adult population in Saskatchewan, Canada: Evidence from predictive modelling
Authors: Muhajarine Nazeem , Adeyinka Daniel A. , McCutcheon Jessica , Green Kathryn L. , Fahlman Miles , Kallio Natalie
Published: November 2021

Background

A high population level of vaccination is required to control the COVID-19 pandemic, but not all Canadians are convinced of the value and safety of vaccination. Understanding more about these individuals can aid in developing strategies to increase their acceptance of a COVID-19 vaccine. The objectives of this study were to describe COVID-19 vaccine acceptance, hesitancy and refusal rates and associated factors in Saskatchewan, Canada.

Methods

This is a cross-sequential study that consisted of pooled responses from weighted samples of 9,252 Saskatchewan adults (≥18 years) across nine rounds of data collection between May 4, 2020 and April 3, 2021. The outcome variable was vaccine intention: vaccine acceptance, hesitancy, and refusal. The independent variables were layered into socio-demographic factors, risk of exposure to coronavirus, mitigating behaviours, and perceptions of COVID-19. Data were analyzed using multinomial logistic regression and a classification and regression tree.

Results

Seventy-six percent of the respondents indicated that they had been or were willing to be vaccinated, 13% had not yet decided, and the remaining 11% said they would not be vaccinated. Factors that increased the likelihood of vaccine refusal and hesitancy were lower education level, financial instability, Indigenous status, and not being concerned about spreading the coronavirus. Perceiving COVID-19 to be more of a threat to one’s community and believing that one had a higher risk of illness or death from COVID-19 decreased the likelihood of both vaccine refusal and hesitancy. Women and newcomers to Canada were more likely to be unsure about getting vaccinated. Respondents who did not plan to be vaccinated were less likely to wear face masks and practice physical distancing.

Conclusion

While many Canadians have voluntarily and eagerly become vaccinated already, reaching sufficient coverage of the population is likely to require targeted efforts to convince those who are resistant or unsure. Identifying and overcoming any barriers to vaccination that exist within the socio-demographic groups we found were least likely to be vaccinated is a crucial component.
Development of an expected possession value model to analyse team attacking performances in rugby league
Authors: Sawczuk Thomas , Palczewska Anna , Jones Ben
Published: November 2021

This study aimed to evaluate team attacking performances in rugby league via expected possession value (EPV) models. Location data from 59,233 plays in 180 Super League matches across the 2019 Super League season were used. Six EPV models were generated using arbitrary zone sizes (EPV-308 and EPV-77) or aggregated according to the total zone value generated during a match (EPV-37, EPV-19, EPV-13 and EPV-9). Attacking sets were considered as Markov Chains, allowing the value of each zone visited to be estimated based on the outcome of the possession. The Kullback-Leibler Divergence was used to evaluate the reproducibility of the value generated from each zone (the reward distribution) by teams between matches. Decreasing the number of zones improved the reproducibility of reward distributions between matches but reduced the variation in zone values. After six previous matches, the subsequent match’s zones had been visited on 95% or more occasions for EPV-19 (95±4%), EPV-13 (100±0%) and EPV-9 (100±0%). The KL Divergence values were infinity (EPV-308), 0.52±0.05 (EPV-77), 0.37±0.03 (EPV-37), 0.20±0.02 (EPV-19), 0.13±0.02 (EPV-13) and 0.10±0.02 (EPV-9). This study supports the use of EPV-19 and EPV-13, but not EPV-9 (too little variation in zone values), to evaluate team attacking performance in rugby league.
Investigating the inflammation marker neutrophil-to-lymphocyte ratio in Danish blood donors with restless legs syndrome
Authors: Dowsett Joseph , Didriksen Maria , Larsen Margit Hørup , Dinh Khoa Manh , Kaspersen Kathrine Agergård , Mikkelsen Susan , Thørner Lise Wegner , Sørensen Erik , Erikstrup Christian , Pedersen Ole Birger , Eugen-Olsen Jesper , Banasik Karina , Ostrowski Sisse Rye
Published: November 2021

Background

Restless Legs Syndrome (RLS) is a neurological sensorimotor disorder that occurs in the evening and night, thereby impacting quality of sleep in sufferers. The pathophysiology of RLS is poorly understood but inflammation has been proposed as possibly being involved. The neutrophil-to-lymphocyte ratio (NLR) can be used as an inflammation marker but results from small studies have been inconclusive in determining whether NLR is associated with RLS. We aimed to assess whether an association between NLR and RLS exists in a large cohort of healthy individuals.

Methods

Neutrophils and lymphocytes were measured in blood samples of 13,055 individuals from the Danish Blood Donor Study, all of whom completed the validated Cambridge-Hopkins RLS-questionnaire for RLS assessment.

Results

In the sample, 661 individuals were determined as current RLS cases (5.1%). A higher proportion of individuals with RLS were females (62.5% vs 47.5%; P<0.001) and RLS cases were older than controls (P<0.001), but no differences in body mass index (BMI), smoking or alcohol consumption were found between the two groups. An increased NLR was observed in RLS cases compared to controls (median NLR: 1.80 vs 1.72; P = 0.033). In an unadjusted logistic regression model, increased NLR was associated with RLS (OR = 1.10 per NLR unit increase [95%CI:1.01–1.20]; P = 0.032); however, the association was not significant in multivariate models adjusting for sex and age (P = 0.094) or sex, age, alcohol consumption, smoking status and BMI (P = 0.107).

Conclusion

We found no association between RLS and NLR among Danish blood donors after adjusting for sex, age, alcohol consumption, smoking status and BMI. Further studies are needed to determine whether inflammation is a risk factor for RLS.
Predictors of slow clinical response and extended treatment in patients with extra-pulmonary tuberculosis in Pakistan, A hospital-based prospective study
Authors: Ambreen Atiqa , Tahseen Sabira , Wali Ahmad , Jamil Muhammad , Naqvi Syed Zeeshan Haider , Safdar Nauman , Mustafa Tehmina
Published: November 2021

The optimal duration of treatment in different forms of extrapulmonary tuberculosis (EPTB) is not clearly defined. This study aimed to identify predictors of slow clinical response and extended anti-TB treatment in EPTB patients. Socio-demographic, clinical, and microbiological characteristics of EPTB patients registered for anti-TB treatment at a tertiary care hospital, were analysed for identification of predictors of extended treatment. A total of 251 patients (137 lymphadenitis, and 114 pleuritis) were included in the analysis. Treatment was extended to more than 6 months in 58/251 (23%) patients. In the multivariate regression analysis, culture-positive EPTB (p = 0.007) [OR (95% CI) = 3.81 (1.43, 10.11)], history of diabetes (p = 0.014) [OR (95% CI) = 25.18 (1.94, 325.83)], smokeless tobacco use (p = 0.002) [OR (95% CI) = 17.69 (2.80, 111.72)], and slow regression of local signs and symptoms after 2 months of treatment (p < 0.001) [OR (95% CI) = 17.09 [(5.79, 50.39)] were seen to be significantly associated with treatment extension. Identification of predictors of extended treatment can help clinical decisions regarding optimal duration of treatment. Further studies are needed to identify subgroups of EPTB patients who can benefit from a shorter or longer treatment regimen.
Early-stage macular telangiectasia type 2 vascular abnormalities are associated with interdigitation zone disruption
Authors: Ong Janice X. , Zandi Roya , Fawzi Amani A.
Published: November 2021

Purpose

To investigate the relationship between disruption in different photoreceptor layers and deep capillary plexus (DCP) telangiectasias in eyes with macular telangiectasia type 2 (MacTel).

Methods

35 eyes (21 patients) with MacTel imaged with optical coherence tomography angiography (OCTA) were included. Circumscribed areas of DCP telangiectasia were traced from OCTA slabs and the corresponding spectral-domain OCT (SD-OCT) slabs were used to visualize the photoreceptor layer interdigitation zone (IZ) and ellipsoid zone (EZ). IZ attenuation, IZ loss, and EZ loss were graded by reviewing en face SD-OCT slabs for hypo-reflective areas and confirming their status on cross-sectional views. Total area of photoreceptor disruption and overlap with DCP telangiectasia were evaluated with respect to OCT-based MacTel stage. Longitudinal changes were evaluated in a subset of patients with follow-up imaging.

Results

Overlap of DCP telangiectasia with IZ attenuation significantly decreased with MacTel severity, while overlap with IZ and EZ loss significantly increased. Overlap with IZ loss peaked in moderate MacTel (Stages 3–5). Longitudinal imaging showed that new EZ loss at 6 months was largely predicted by baseline IZ loss.

Conclusions

Worsening MacTel severity is characterized by greater overlap between DCP telangiectasia and zones of increasing severity of photoreceptor disruption, with EZ loss enlarging over time within areas of preexisting IZ disruption. We suggest that IZ disruption may indicate early photoreceptor dysfunction that eventually progresses to EZ loss, with IZ loss being a more reliable metric than IZ attenuation. Additional studies will be necessary to further explore long-term photoreceptor changes and evaluate their relationship with visual function in MacTel.
Consumer empowerment and self-assessment of empowerment
Authors: Nam Su-Jung
Published: November 2021

This study examined the influence of consumer empowerment and its self-assessment on consumers’ information search behavior and consumer life satisfaction; it also examined whether the results were consistent with the Dunning−Kruger effect. A total of 977 consumers who participated in a national consumer survey were divided into four groups, based on their level of empowerment and self-assessment. The Dunning−Kruger effect was observed in the consumer empowerment results, with 35.9% of respondents showing imbalanced empowerment and self-assessment levels. A general linear model was used to examine the survey results, which indicated that the main effect of empowerment had no significant effect on information searching or consumer life satisfaction. However, there was a significant main effect of self-assessment on both dependent variables. In addition, the interaction of empowerment and self-assessment had a significant effect only on information search behavior. Consequently, it can be concluded that self-assessed empowerment, rather than actual consumer empowerment, affects information search and consumer life satisfaction.
Suitable nitrogen application mode and lateral spacing for drip-irrigated winter wheat in North China Plain
Authors: Zain Muhammad , Si Zhuanyun , Chen Jinsai , Mehmood Faisal , Rahman Shafeeq Ur , Shah Adnan Noor , Li Sen , Gao Yang , Duan Aiwang
Published: November 2021

To propose an appropriate nitrogen application mode and suitable drip irrigation lateral spacing, a field experiment was conducted during 2017–2018 and 2018–2019 growing seasons to quantify the different drip irrigation lateral spacings and nitrogen fertigation strategies effects on winter wheat growth, yield, and water use efficiency (WUE) in the North China Plain (NCP). The experiment consisted of three drip irrigation lateral spacing (LS) (40, 60, and 80 cm, referred to as D40, D60, and D80 respectively) and three percentage splits of nitrogen application modes (NAM) (basal and top dressing application ratio as 50:50 (N50:50), 25:75 (N25:75), and 0:100 (N0-100) respectively). The experimental findings depicted that yield and its components, and WUE were markedly affected by LS and NAM. Fertigation of winter wheat at N25:75 NAM notably (P<0.05) increased the grain yield by 4.88%, 1.83% and 8.03%, 4.61%, and WUE by 3.10%, 3.18% and 5.37%, 7.82%, compared with those at NAM N50:50 and N0:100 in 2017–2018 and 2018–2019 growing seasons, respectively. LS D40 appeared very fruitful in terms of soil moisture and nitrogen distribution, WUE, grain yield, and yield components than that of other LS levels. The maximum grain yield (8.73 and 9.40 t ha-1) and WUE (1.70 and 1.95 kg m-3) were obtained under D40N25:75 during both growing seasons, which mainly due to that all main yield components in D40N25:75 treatment, such as spikes per unit area, 1000-grain weight, and grains per spike were significantly higher as compared to other treatments. The outcomes of this research may provide a scientific basis of lateral spacing and nitrogen fertigation management for the production of drip-irrigated winter wheat in NCP.
Biotinylation as a tool to enhance the uptake of small molecules in Gram-negative bacteria
Authors: Pandeya Ankit , Yang Ling , Alegun Olaniyi , Karunasena Chamikara , Risko Chad , Li Zhenyu , Wei Yinan
Published: November 2021

Antibiotic resistance is a major public health concern. The shrinking selection of effective antibiotics and lack of new development is making the situation worse. Gram-negative bacteria more specifically pose serious threat because of their double layered cell envelope and effective efflux systems, which is a challenge for drugs to penetrate. One promising approach to breach this barrier is the “Trojan horse strategy”. In this technique, an antibiotic molecule is conjugated with a nutrient molecule that helps the antibiotic to enter the cell through dedicated transporters for the nutrient. Here, we explored the approach using biotin conjugation with a florescent molecule Atto565 to determine if biotinylation enhances accumulation. Biotin is an essential vitamin for bacteria and is obtained through either synthesis or uptake from the environment. We found that biotinylation enhanced accumulation of Atto565 in E. coli. However, the enhancement did not seem to be due to uptake through biotin transporters since the presence of free biotin had no observable impact on accumulation. Accumulated compound was mostly in the periplasm, as determined by cell fractionation studies. This was further confirmed through the observation that expression of streptavidin in the periplasm specifically enhanced the accumulation of biotinylated Atto565. This enhancement was not observed when streptavidin was expressed in the cytoplasm indicating no significant distribution of the compound inside the cytoplasm. Using gene knockout strains, plasmid complementation and mutagenesis studies we demonstrated that biotinylation made the compound a better passenger through OmpC, an outer membrane porin. Density functional theory (DFT)-based evaluation of the three-dimensional geometries showed that biotinylation did not directly stabilize the conformation of the compound to make it favorable for the entry through a pore. Further studies including molecular dynamics simulations are necessary to determine the possible mechanisms of enhanced accumulation of the biotinylated Atto565.
Concubare sine mea volumtate: denuncias y procesos por violación en el noroeste ibérico (siglos VIII-XII)
Authors: Lorenzo-Rodríguez Abel
Published: November 2021

En el siguiente trabajo se analizará un tipo de violencia concreta, la sexual, que afecta a mujeres. A través de una significativa muestra documental entre los siglos X y XII del noroeste ibérico se mostrarán las principales actitudes sociales ante la violación, tanto por parte de los agresores, las víctimas y los encargados de regular el crimen. Para ello, primeramente, se recurrirá a un estudio léxico para definir la realidad de la violación y las formas en la que la podemos cotejar a través de los documentos. También crónicas, hagiografías y sermones contemporáneos serán utilizados para ofrecer una perspectiva del crimen sexual en los discursos de poder. La muestra comprende desde documentos en los que la mujer denuncia en primera persona hasta referencias a reconocimientos de culpa de los agresores. El objetivo es saber cómo la mujer puede resistir al abuso, denunciar los hechos y las consecuencias para los agresores